LOCUS       DASPJI010000001         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_800952, whole genome shotgun sequence.
ACCESSION   DASPJI010000001 DASPJI010000000
VERSION     DASPJI010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800952"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VEL74_00005"
     CDS             <1..217
                     /locus_tag="VEL74_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60934.1"
                     /translation="TTASTGSAKSATAGTSKADKKAGTSSAKKSESKPAKTSAKSGKK
                     AEASGKTAAKKASSSGAGKGAAKKKSS"
     gene            complement(321..1433)
                     /locus_tag="VEL74_00010"
     CDS             complement(321..1433)
                     /locus_tag="VEL74_00010"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HYG60935.1"
                     /translation="MNSSDPLSFLRDAVAQVDRAMLELLRHRLELAAEIGRIKAEAGL
                     PVVVRDVETSVLSRARQHAEACGVSEEVMEAVFQAIIRGSVERQYRVGIARRELHGER
                     VLIVGGAGGMGTWFRAFLELEGHNVEVADPALATLPATPGRFGGLSLVEDLDRYAAIL
                     VAVPLDRTGEVVQQVVERSPRGLVLEVASIQTPVKEALLLARQRGVRALALHPMFGPG
                     KSLYEPLTFVLATLGDPQEEKHWLGELLSHPYTRLVTVPFEHHDRLMGWLLGLAHLTN
                     MLFGTALIRSGVDPSELHACASTTFTRQAATALKVLSEDPDLYIDIQHLNPHRHEVYN
                     AAREALDQLEPLVERRDREGFRKILAAARRALTGDA"
     gene            1893..>2799
                     /locus_tag="VEL74_00015"
     CDS             1893..>2799
                     /locus_tag="VEL74_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HYG60936.1"
                     /translation="MPKHSTPTPATAPASGKQFFGHPLGLSTLFFTEFWERFSYYGMR
                     ALLILFMTASAEAGGLGFSTEKAGAIYGLYTAFVYLLALPGGWIADQLIGQRRAVFWG
                     GVIIAAGHFSMAIESMTTFYLGLVLIVIGTGLLKPNVSTMVGELYPEGGARRDAGFSI
                     FYMGINLGAFVAPLICGPLGQRINWHWGFAAAGFGMVIGLIQYAIMGRFLGEAGLHPN
                     AAGDPAARAKGFRILWIAGLVTVVAGLLIATGTLRISAQMLANAAGVIIVSLALIYLI
                     YQLVAGGLDATEKKRMIVIFVLFIFS"
BASE COUNT          462 a          954 c          935 g          448 t
ORIGIN      
        1 cacgaccgcc agcaccggca gcgccaagtc cgccacggcc ggcacctcga aggccgacaa
       61 gaaggccggg acgagctccg ccaagaagtc cgagagcaag ccggcaaaga cgtctgccaa
      121 aagtggcaag aaggccgagg cgtccggcaa gaccgcggcc aagaaggctt cgagctccgg
      181 ggcaggaaag ggcgccgcga agaagaagag ctcctgacct cgtccctgct cacctctctc
      241 gagcgaggcc ctcgctcatc gcgatgcggt gagcagggcc tcgcggtcgt ttcagacccc
      301 gcaagaaaac tgatcaatct ctaggcgtcc ccggtcagcg cccgtcgcgc cgcggccagg
      361 atcttgcgaa agccctcccg gtcccgccgc tcgaccagcg gctccaactg atcgagagcc
      421 tcgcgggcgg cgttgtagac ctcgtggcgg tgcgggttca ggtgctggat gtcgatgtag
      481 aggtccgggt cctcggagag caccttcaag gcggtggccg cctggcgggt gaacgtcgtc
      541 gaggcgcagg cgtgcagctc cgacggatcg actcccgagc ggatgagggc ggtgccgaac
      601 agcatgttgg tcaggtgggc gagacccagg agccatccca tcaggcggtc gtggtgctcg
      661 aacggcaccg tcaccaggcg ggtgtagggg tgtgacagca actcgccgag ccagtgcttc
      721 tcctcctggg ggtcgccgag agtcgcgagg acaaaggtga ggggctcgta gagcgacttg
      781 ccggggccga acatcgggtg cagggcgaga gcgcggacgc cgcgctggcg ggcgagcaac
      841 agcgcctcct tgaccggtgt ctgaatggag gcgacctcca gtaccagccc acggggagac
      901 cgctccacca cctgctggac cacctcgccc gtgcggtcca gcggcaccgc caccaggatg
      961 gcggcgtagc ggtcgagatc ctccaccagg ctcaggccgc cgaaacgccc gggcgtcgcg
     1021 ggcagcgttg ccagggcggg gtcggctacc tcgacgttgt gaccctccag ctccaggaac
     1081 gcgcggaacc aggtgcccat accgccggcg cctccgacga tcagcacccg ttcaccgtgc
     1141 agttcccgcc gtgcgatccc cacccggtac tggcgttcca cggagccgcg gatgatcgcc
     1201 tggaacaccg cttccatcac ctcttcggag acgccgcagg cctccgcgtg ctggcgggcg
     1261 cggctcaaca cgctcgtctc gacgtcgcgc accaccaccg ggagcccggc ctcggccttg
     1321 atgcggccga tctcggcggc gagctccaac cggtgacgca acaactccag catggcgcgg
     1381 tccacctggg ccacggcatc gcgaaggaac gaaagaggat ctgatgaatt catagaacag
     1441 gtctcccgca gtgggtacgt tctatggagg atggtgcgcc ggaggagggg aggggagccg
     1501 gcttgcgccg gccccccctt gagatgccgc gcggcgtacg gctgccgcgg gtcggatggg
     1561 cctcggtcaa ctgccagacg cttttcatat agagcaagct cgatgccata gccctggcgc
     1621 gccgtgcctc ctcggggaag ggaccgggag agggcccggc gaccgctccc ggtggactgt
     1681 ttcgtgaaca ctcccgtcct aacgtgtgga caggggacga gaggagccgg gtgtgtccgc
     1741 cgcctggcac ctgccttgct gctctccgcc ccgatgacac tgtgctagtc tcccgcgcgc
     1801 ggaagcccga gacgtgcagg gtgccaatgg acgaggcggc gatgggggcg ggccccctgg
     1861 ctgatctcgg caacgaccat caagagacac tgatgcccaa acattcgacc cctactccgg
     1921 ccaccgcccc tgcctcgggg aaacagttct tcggccaccc actcggtctc tccaccttgt
     1981 tctttaccga attctgggag cgcttcagct actacggcat gcgggcgctg ctcatcctct
     2041 tcatgacggc gtcggccgag gcgggagggc tgggcttctc gaccgagaag gcgggcgcca
     2101 tctacggcct ctacacggcc ttcgtctatc tcctcgcgct gccgggcggc tggatcgccg
     2161 accagctcat cggccagcgg cgcgccgtgt tctggggcgg agtcatcatc gcggccggtc
     2221 acttcagcat ggccatcgag agcatgacga cgttctatct cgggctcgtg ctgatcgtca
     2281 tcggcaccgg cctgttgaag cccaatgtca gcacgatggt gggcgagctc tatcccgaag
     2341 gcggggcccg ccgcgacgcc ggcttctcga tcttctacat ggggatcaac ctcggagcct
     2401 tcgtggcccc cctgatctgc ggcccgctgg ggcagcggat caactggcac tggggcttcg
     2461 ccgccgccgg cttcggtatg gtgatcggcc tgatccagta cgccatcatg gggcgcttcc
     2521 tcggcgaagc gggccttcat ccgaacgccg cgggcgatcc cgcggcacgg gcgaagggct
     2581 tccggatcct ctggatcgcc ggcctcgtca ccgtcgtggc aggcctcctg atcgccaccg
     2641 gcaccctccg gatctcggcg cagatgctgg ccaacgccgc gggcgtcatc atcgtctcgc
     2701 tggcgctgat ctatctgatc tatcagctcg tggccggcgg cctggacgcc accgagaaga
     2761 agcggatgat cgtcatcttc gtcctcttca tcttctcgg
//
LOCUS       DASPJI010000002         7372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_400716, whole genome shotgun sequence.
ACCESSION   DASPJI010000002 DASPJI010000000
VERSION     DASPJI010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400716"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VEL74_00020"
     CDS             complement(<1..747)
                     /locus_tag="VEL74_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60937.1"
                     /translation="MGDGHPGPPAEEWSSVERLLQESGLDLQTATRPTWWPLQPEGET
                     DEPELEPQAGGLYDEVFDRVFQRVASQESVVARERARGRDLFEELVRHPPARQLLLIR
                     NSTRYRSPMLCEHLIEECQAEGFKDVFRALSLARLAVAVADLLTPADCVGEESLASLK
                     ARTWAQLGNACRINADHTGAEQAFEQARAIGQTGGIELLAKARILDLEASLRRDQRRF
                     QEAFQLLDEVISIYHQLGQRNLLGRSLKQKS"
     gene            complement(740..1276)
                     /locus_tag="VEL74_00025"
     CDS             complement(740..1276)
                     /locus_tag="VEL74_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG60938.1"
                     /translation="MSALDHPHVETSLEEFLQRVRPRLKTLFARYRIPPQDTEDILQQ
                     ALLALVYQRQNIRDAESWLIGTLRNKCLLYWRDHRRKLYDAVDAAVLEIVAEPLAPAQ
                     EDADLRRDLADAIERLPERCRSLLSLRYRHGYEPPELAERLGYSPASISKITTRCLAA
                     LSRHLVSAGLGKRKTNHG"
     gene            complement(1379..1672)
                     /locus_tag="VEL74_00030"
     CDS             complement(1379..1672)
                     /locus_tag="VEL74_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60939.1"
                     /translation="MSQRNSRHVAAALTLAVFLSVLPFSELDAAPRVRSPRGQDLWQR
                     VEQQVSQLLGGVGRVWQKVGARIDDNGFRFRLGGAEVPDDGLKPAEGPTPPER"
     gene            complement(1827..2312)
                     /locus_tag="VEL74_00035"
     CDS             complement(1827..2312)
                     /locus_tag="VEL74_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG60940.1"
                     /translation="MKRVEPRLKRILFRYKIPAQDAEDLLQETFLIMVSKSGSIRNPE
                     AWLLATLSNRCVIYWRRHRSRLWDLVDTTILELLAESEAPTQELSDLRSDLDALLAQL
                     PDRCRSVLRLRYGLGCSTAEAAERMGYCTSSIRKVTRRCLAALTEQLLGAGAQPGSLA
                     D"
     gene            complement(2484..3836)
                     /locus_tag="VEL74_00040"
     CDS             complement(2484..3836)
                     /locus_tag="VEL74_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60941.1"
                     /translation="MARHLDPTNLEELRRGRLSRGKSRDLVRHLLAGCEACLTLAARF
                     LPAGPGAAGADSAPEARYSEVFDKVRQELAERQAALSVEQGEAPGILRELLQHPLDRQ
                     RLLIANSPRFRTWALCELLLEAAREEGFQDPAKALELARLGALTAEHLDASLYGDGRA
                     RDLAARAWATVGNAERIRSDFQAAEKSFQRAERLLKQGTGDPLEKAGVLLLKASLLGN
                     QQRFPEAFRVLDRVVSIARKYGDLHLCGKAMVTRGFLCGVAHDLPEEEASRWLSDGLA
                     LVDPAAEPRLVVAARHNLILHLAECGRNAEALQLLEQTRPLYAFMGDKMNLLRLRWVE
                     GRIAVAQGQFARAEPMLQEIRRELVEREIGYDAALVSLDLARIYARQGRSAEMRRLAE
                     EMLPIFQSRQIQREALTALVIFQKAAEMESVTLGLVQELSEYLDRTRKHSGSRFSESV
                     "
     gene            complement(3829..4374)
                     /locus_tag="VEL74_00045"
     CDS             complement(3829..4374)
                     /locus_tag="VEL74_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYG60942.1"
                     /translation="MATLDHPELRESLNTLLKNSRPKLERVLRNFEIPPEDAEDILQD
                     AQLTLLYKWDKIRSPESWLIGTVKKKCIMYWRKRRGSLCEAVDTAILDLLALPQPPDQ
                     EKKDLTCDLNRVLGRLSSRCRSLLKLRYGLGYGPTEVAEQMGYRTSSIRKVTNRCLAA
                     LTRQLLAVGFDRELQGEPQDG"
     gene            complement(4493..4804)
                     /locus_tag="VEL74_00050"
     CDS             complement(4493..4804)
                     /locus_tag="VEL74_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60943.1"
                     /translation="MRSMRRPLVVLALCLVLAFVPVASHAAAHAAPLALPNVPAFGLG
                     AVLGDAWSAVQSFMSKILVSGGGDDDGTTSTTQGTPPTGSGTPPAGGTGDNGPGIDPN
                     G"
     gene            complement(4994..6193)
                     /gene="metK"
                     /locus_tag="VEL74_00055"
     CDS             complement(4994..6193)
                     /gene="metK"
                     /locus_tag="VEL74_00055"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HYG60944.1"
                     /translation="MTQREAHLFTSESVTEGHPDKIADQISDAILDDVLRQDPTGRVA
                     CETTVTTGMVFLAGEISTKAYVDFASIARQVVSDIGYTRAEYGIDGETCAVLSAIDPQ
                     SPDIAQGVDTGGAGDQGLMFGFACRETEHLMPMPITLAHDLTRRLAAVRRSGELPWLR
                     PDGKSQVTIEYDGIKPARVHTVVVSTQHDPEVSQEDIVEGVIEKVIRPVIAPELLDDR
                     TVFHINPTGRFVIGGPKGDCGLTGRKIIVDTYGGYGRHGGGAFSGKDPTKVDRSACYM
                     ARHIAKNLVAAGIAERVEVQLAYAIGVADPVSVRADTFGTGTISDRHIERLIRDQFPL
                     SPGGIIEYLDLRRPIYRKTAAYGHFGRGEEDGFTWESVRRAPELRAAAGLSAAGVSGV
                     PSMAASA"
     gene            complement(6390..6983)
                     /locus_tag="VEL74_00060"
     CDS             complement(6390..6983)
                     /locus_tag="VEL74_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF84 family protein"
                     /protein_id="HYG60945.1"
                     /translation="MSTDLKNFWQRLQRGVEVAVAGHTPDKLLGVRDGLLRFFHDGLD
                     RIVSVAVVPQTVEEAPVGLPFSDEEVLRLARQRVVELEKSLADTYHFYIATEGGIHSL
                     EVDGKVCYFVRNWTVVRGLLGEAWGSSGSIQLPDRLVAGLDSDQVPFAVPGTRKSGGM
                     ISSLTGGLETRRRAIAVSTLHCISTLFYGVLESRPVR"
     gene            6982..>7372
                     /locus_tag="VEL74_00065"
     CDS             6982..>7372
                     /locus_tag="VEL74_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60946.1"
                     /translation="MIVSRQNRRLKDIRRLRRSKGAQALLEGPHLVAEALAGGLALET
                     ALATPDFLASSEGAGLARGLHGRGVEILEVEAGLLAELTDADSPRGILAVASLPRRGV
                     DALPVRDGAAYLYLEGLQDPGNLGALAR"
BASE COUNT         1180 a         2447 c         2584 g         1161 t
ORIGIN      
        1 cgatttctgc ttgaggctcc gtcccagcag attgcgctgc ccgagctggt gatagatgga
       61 gatcacctcg tccaggagct gaaaggcctc ctggaagcgg cgctggtcgc gccggagtga
      121 ggcttcgagg tccagaatgc gggccttggc gaggagctcg atcccgcctg tctggccgat
      181 ggcgcgagcc tgctcgaagg cctgctcggc tccggtgtga tccgcgttga tacggcaggc
      241 attcccgagc tgtgcccagg tcctggcctt caggctggcg agcgactctt caccgacaca
      301 gtccgccggg gtgagcaggt cggcgaccgc cacggccagc cgagccagcg acagagcccg
      361 gaacacatcc ttgaaaccct cggcctggca ctcctcgatc aggtgctcgc agagcatcgg
      421 gctccggtag cgcgtgctgt tcctgatcag gaggagctga cgggcgggcg ggtgccgcac
      481 gagctcttcg aagagatcgc gcccccgggc ccgctcccgg gccaccacgg actcctggga
      541 agccaccctc tggaagacac gatcgaagac ctcgtcatag agaccgccgg cctgtggctc
      601 cagctccggt tcgtcggtct ctccctcggg ctgcagcggc caccaggtag gcctggtcgc
      661 cgtctgaagg tcgagaccgc tctcctggag gaggcgctct acgctgctcc actcttcggc
      721 aggcggtcca gggtgcccgt cacccatggt tcgtcttcct tttcccgagt cccgcggaga
      781 ccaagtgccg gctcaaggcc gccaggcacc gggtggtgat cttgctgatg ctcgcggggc
      841 tgtagccaag gcgctccgcc aactccggcg gctcgtaccc atgccggtag cgcagggaga
      901 ggagcgagcg gcaacgctcc ggcagccgct cgatggcgtc cgccagatcc cgccgcaggt
      961 ccgcgtcctc ctgggccggc gccaaaggct cggcgacgat ctccagaacc gccgcatcca
     1021 ccgcgtcgta gagcttgcgc cggtggtccc gccagtacag caggcatttg ttccgcaacg
     1081 tgccgatcag ccacgactcg gcatcccgga tgttctggcg ctggtacacc agagccagca
     1141 gcgcctgctg caggatgtcc tccgtgtcct ggggagggat ccggtagcgc gcgaagagcg
     1201 tcttgagacg gggacgcacc ctttgcaaaa actcttcgag agaggtctcg acgtgcggat
     1261 ggtccagtgc actcatcgac aggaggaggt tttgaatgag ttgatacact accaccctcc
     1321 tggattcttg tccagccgcg cgctcctttt tcgtgccgga tgccccggcg gccctcccct
     1381 acctttccgg gggcgtgggg ccttcagccg gcttcagacc gtcgtccgga acctcggcgc
     1441 ctcccagacg gaagcggaat ccgttgtcat cgatgcgggc cccgaccttc tgccagacac
     1501 gccccacccc acccaagagt tgcgagacct gctgctcgac ccgttgccag agatcctggc
     1561 cgcgaggaga gcgtacccgg ggggcagcgt cgagctccga gaaggggagc acggagagga
     1621 agaccgccag ggtgagagcg gcggcgacgt gacgactgtt gcgctgagac atcgtatctc
     1681 ctttctcaag acctccggag ctccagaggc ttgcgtccaa caatagagtt tccgcacacg
     1741 ggaaagcgag gacaggaaga cggagccaac ctgccacgca ctgacagagt gcgcgaaact
     1801 gctcggaaag ggaaaggatg ccgcgattag tcggccaggg agccgggctg ggcgcctgcc
     1861 ccgagcagct gctcggtcag cgcggcgagg cacctgcggg tcactttgcg gatgctggag
     1921 gtgcaatagc ccatccgctc tgcggcctcc gccgtgctgc agcccagtcc atagcgcagg
     1981 cggaggaccg accggcaccg gtccgggagc tgcgccagca gcgcgtccag atcggatcgg
     2041 aggtcggaga gctcctgggt cggcgcctcg gattcggcga gcagctcgag gatcgtcgtg
     2101 tcgacgaggt cccacagccg gctcctgtgc ctgcgccagt agatgacgca acggttggag
     2161 agggtggcga gcagccaggc ctccggattg cggatgctcc ccgacttcga caccatgatg
     2221 aggaaggtct cctgcaggag gtcctcggcg tcttgggccg gtatcttgta gcggaagagg
     2281 atccgcttga gcctcggctc aaccctcttc aagacctcct cgagggagag gggctccagg
     2341 caagggggag attccatcgc agtcgcttta aatgtggagg gtaacctacc aagtctcccg
     2401 gtgtttgtcc agcttcagga gcggcagcga gggctcgctc ccccactcgg gcggcggcgg
     2461 aaggcggacc ctctcgatcg ggctcagacg gactcgctga agcggctacc ggaatgcttg
     2521 cgggtccggt ccaggtactc gctcagctcc tggaccagac cgagcgtgac gctctccatc
     2581 tcggccgcct tctggaagat gaccagcgcg gtgagagcct cgcgctggat ctgacgcgac
     2641 tggaagatgg gcagcatctc ctcggcgagg cggcgcatct ccgcgctgcg cccctggcgg
     2701 gcatagatgc gggccaggtc gaggctgacc agggcggcgt catagccgat ctcgcgctcc
     2761 acgagctccc tgcggatctc ctggagcatc ggctcggcgc gggcgaactg cccctgcgcc
     2821 acggcgatcc tgccctccac ccaacggagg cgcaggaggt tcatcttgtc gcccatgaag
     2881 gcgtagagag gccgtgtctg ctccagcagc tggagcgcct cggcattccg cccgcattcg
     2941 gcgaggtgaa ggatgaggtt gtgccgggcc gcgaccacga gcctgggctc ggcggcggga
     3001 tcgaccagag cgagcccgtc ggagagccag cggctcgcct cctcctcggg caggtcgtga
     3061 gcgacgccgc agaggaagcc ccgggtgacc atcgccttgc cgcacaggtg caggtccccg
     3121 tacttgcgag cgatcgacac gacccggtcg agcacgcgga acgcctcggg gaagcgctgc
     3181 tggttgccga ggagcgaggc cttgaggagg agcacccccg ccttctccag gggatcgccg
     3241 gtcccctgct tgaggaggcg ctcggcccgc tggaagctct tctccgcggc ctggaagtcg
     3301 gagcggatgc gctcggcatt ccccacggtc gcccaggccc gggcggcgag gtcccgggcg
     3361 cggccgtcgc catagaggct ggcgtcgagg tgctcggccg tcagggcgcc caaccgagcc
     3421 agctccagag ccttcgcggg atcctggaaa ccctcttccc gcgccgcctc caggagcagc
     3481 tcgcacagcg cccaggtgcg gaaacgggga ctgttggcga tcagcagccg ctggcggtcg
     3541 agggggtgct gcaagagctc ccggaggatc cccggcgctt cgccctgctc caccgacagc
     3601 gcggcctgcc gctctgccag ctcctgccgc accttgtcga agacttcgga gtacctcgct
     3661 tccggcgcgg aatccgcccc ggcggctccc ggaccggcag gcaggaagcg cgcggccagc
     3721 gtcaggcagg cctcacagcc cgcgagcaga tgccggacga ggtcccggct cttgcctcgg
     3781 gacaggcggc cccgccgcag ctcctccaga ttggtgggat ccaggtgtct agccatcttg
     3841 aggctcccct tgcaactccc tgtcgaaacc gaccgccagc agctgccggg tcaacgcggc
     3901 caggcagcgg ttggtgactt tccggatgct ggaggtcctg tatcccatct gctccgccac
     3961 ttcggtcggc ccgtagccga ggccgtagcg gagcttgaga agggaccggc agcgggagga
     4021 gagacggccg agaacacggt tcagatcgca ggtcaggtcc ttcttctcct ggtcgggagg
     4081 ctgcgggagc gccagcaggt ccaggatggc ggtgtccacc gcctcacaga ggctcccccg
     4141 gcgcttgcgc cagtacatga tgcacttctt cttgaccgtg ccgatcagcc agctctcggg
     4201 gctgcggatc ttgtcccact tgtagagaag agtgagttgg gcatcctgca ggatgtcctc
     4261 cgcgtcttcc ggcgggatct cgaaattgcg aagaacccgc tccagcttcg gacgggagtt
     4321 cttcaggaga gtattgagcg attcccgaag ttcaggatga tccagcgttg ccatagacgc
     4381 gatcggtacc ttgacgtccc gctaggctaa caccccggtc gccccctgtc cagatggaac
     4441 aggactccgc cgtatgaagg ccgggaggga ggggccggga gcccgccggg cactagccgt
     4501 tggggtcgat cccggggccg ttgtcgccgg tcccgccggc gggcggcgtg ccgctgccgg
     4561 tcggcggcgt gccctgggtc gtggaggtgg tcccgtcgtc gtcaccgccg ccggagacaa
     4621 ggatcttgct catgaagctc tggacggcgg accaggcgtc cccgaggacc gcgccgagtc
     4681 cgaaggccgg gacattcggc agggccagcg gggcggcgtg cgcggcggcg tgcgaggcga
     4741 cggggacgaa ggcgagcacg aggcacagag cgaggacgac gagaggacgg cgcattgaac
     4801 gcatgtttcc ctcctgcgaa ggttcttcaa ccgcttgtcg tgaggctgct aaaaaggata
     4861 tgtggcctgg ccggatagct ccgggacaca cttccttttc gcagcgcggc agagttggaa
     4921 caggaggaga tcttaaaaca aaaacggcgg gcgcaagcac ccgccgtcga aattcgctgg
     4981 aatctctcgc ggatcaggcc gaggcggcca tggacggcac acccgagacg ccggcggcgg
     5041 agaggccggc cgcggcgcgc agctccggag cccggcgcac ggactcccag gtgaacccgt
     5101 cctcctcgcc gcgcccgaag tgcccgtagg cggccgtctt gcggtagatg ggacggcgca
     5161 ggtcgaggta ctcgatgatg ccgcccggcg acagggggaa ctggtcgcgg atcaggcgct
     5221 cgatgtggcg gtcgctgatg gtgccggtgc cgaaggtgtc ggcccggacg gagaccgggt
     5281 cggccacgcc gatggcgtag gcgagttgga cctcgacccg ctcggcgatg ccggccgcca
     5341 ccaggttctt ggcgatgtgg cgcgccatgt agcaggcgga gcggtccacc ttcgtcggat
     5401 ccttgcccga gaaggcgccg ccgccgtgcc ggccgtagcc gccgtaggtg tcgacgatga
     5461 tcttgcggcc ggtgaggccg cagtccccct tcgggccgcc gatgacgaac cggccggtgg
     5521 ggttgatgtg gaacacggtg cggtcgtcca gcagctcggg cgcgatcacc ggcctgatga
     5581 ccttctcgat caccccctcg acgatgtcct cctgcgacac ttcggggtcg tgctgggtcg
     5641 agacgaccac ggtgtgcacc cgggccggct tgatgccgtc gtactcgatg gtgacctggc
     5701 tcttgccgtc cggccgcagc caggggagct cccccgagcg ccgcaccgcc gcaaggcggc
     5761 gggtcaggtc gtgggccagg gtgatgggca tcggcatcag gtgctcggtc tcgcggcagg
     5821 cgaagccgaa catcaggccc tggtcgccgg cgccgccggt gtcgaccccc tgggcgatgt
     5881 ccggcgactg cggatcgatg gcggacagca ccgcgcaggt ctcgccgtcg atcccatact
     5941 ccgcccgggt gtagccgatg tcggagacca cctggcgggc gatgctggcg aagtcgacgt
     6001 aggccttggt gctgatctca ccggcgagga acaccatgcc ggtggtcacc gtggtctcgc
     6061 aggcgacccg gccggtgggg tcctggcgca ggacgtcgtc caggatggcg tccgagatct
     6121 gatcggcgat cttgtcgggg tgcccctccg tcaccgactc ggaggtgaac aggtgagctt
     6181 ctcgttgggt catcgtcaca cctcaagcat ttgtcggacg aaccagggct cgtttgtgcc
     6241 gggctcgaag ccgaggagag aaaaggaaga cacacagcca ccgtgtcggc cggggctgca
     6301 tgctaccaaa gtggtcaaaa atgggtcaac cgggacgccg cccccgcggc cgcggtccgg
     6361 ctggcggccg ggccgggcgc ggggccctgc tagcggaccg gccggctctc gagcacgccg
     6421 tagaagagag tcgagataca gtgcagggtc gagaccgcga tcgcccgccg ccgcgtctcg
     6481 aggccgccgg tgagcgagct gatcatgccg ccgctcttgc gggtgcccgg tacggcgaac
     6541 gggacctgat cgctgtcgag cccggcgacg agccggtccg ggagctggat ggagccgctc
     6601 gagccccagg cctctcccag gaggccgcgc accacggtcc agttgcgcac gaagtagcag
     6661 accttgccgt ccacttcgag cgagtggatg ccaccctcgg tggcgatgta gaagtggtag
     6721 gtgtccgcca gggacttctc cagctcgacg acgcgttgcc gcgccagccg gagaacctcc
     6781 tcgtcggaga acgggaggcc cacgggggcc tcctcgaccg tctgggggac cacggcgacc
     6841 gagacgatcc ggtcgaggcc gtcgtggaag aagcggagga ggccgtcacg caccccgagg
     6901 agcttgtccg gagtgtggcc cgccaccgcc acctcgacgc cccgttggag cctttgccag
     6961 aagttcttaa gatccgtgct catgatcgtc agtcgtcaaa atcgaaggct gaaggatatt
     7021 cggcgcctcc ggcgaagtaa aggagcgcag gcgctgctgg agggccccca cctggtcgcc
     7081 gaggctctcg ccggcgggct ggcgctggaa acggcgctgg cgaccccgga cttcctggcc
     7141 tcctccgaag gggccgggct ggcccgcggc ctccacggcc ggggggtaga gatcctggag
     7201 gtggaggccg ggctgctcgc cgaactgacc gacgccgact ccccccgcgg catcctggcg
     7261 gtggcctccc tcccccgccg gggcgtcgac gcgctgccgg tgcgcgacgg cgccgcctac
     7321 ctctacctcg aggggctcca ggacccgggg aacctcggcg ccctggcccg gg
//
LOCUS       DASPJI010000003         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_332, whole genome shotgun sequence.
ACCESSION   DASPJI010000003 DASPJI010000000
VERSION     DASPJI010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2199
                     /gene="cphA"
                     /locus_tag="VEL74_00070"
     CDS             <1..2199
                     /gene="cphA"
                     /locus_tag="VEL74_00070"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HYG60947.1"
                     /translation="EAISEAMLWVALVQLGHGKYQRRIQATTTSKTQSIASEIASDKR
                     LTRQILDQLGLPVPRQEVVYDAGEAVEEAERIGYPVVLKPLDGNQGRGVSINLKDAEQ
                     VRAAFGVARDISRGVIVESFQPGNDYRILVVDGRVIAVAQRVPGHVVGDGRHTIAELV
                     ETVNRDPRRGVGHEKTLTRLEIDHQAHRLLAQAGLTLESVLPEGEVFYLRSTGNLSTG
                     GTSIDKTDAIHYDNRIMAERGVKAIGLDVAGVDFVSPDISRSYKEVGGAIVEINAAPG
                     FRMHTAPTEGKARDVAGPVIDMLFPNGTPCRIPIAAITGTNGKTTTTRMVGHILKLAG
                     HTVGMTTTDGVYIDGVLTVRGDMTGPWSSQLVLRDPTIDTAVLETARGGILRSGLGWR
                     RCSVGAVLNVAADHLGLAGIDSVDDLAKVKQVVVEVAQDYCVLNADDPRVARMAEQSP
                     AEPIYVTMDRTNELVRKHVLKGGKAVALEEGLSGAMLALYQGEEQIPLVLARQIPATV
                     EGRARHNVQNAMFAAAIAVGMGVDLEDIRQGLRTFSTDFFQAPGRLNFYNEYPFRVLL
                     DYAHNAHGMEAVARMVRELAVHGRRIGVIGAPGDRRDEDIRATAAAAAPAFDLMLLRE
                     DDDLRGRQPGEVADLLREGLVGAGFPAERIIPKVHEESEAVESAMEMARAGDLVVIFG
                     DRVTRDWELIVGFGKKVGIRRRDEPAVLMEAPEEPVLAGMGGEDRELRAG"
     gene            complement(2258..>3011)
                     /locus_tag="VEL74_00075"
     CDS             complement(2258..>3011)
                     /locus_tag="VEL74_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60948.1"
                     /translation="NGGGNTGGALKLDVRPDVWNLNWENAAGNLQAFVRGRDAGKIDL
                     DSIELSGSEDEDKSISPRSTRFAGGQVVATFSKSDAIGLFEDAKPGQRRTVTLTFEIE
                     GEDEAQELTDQVRIVGKPDGGGGEGEGEGEDELHLDIQPGSWNTNWERSLGQVHVFLR
                     GSDARDIDLRSIVLVGTDEEAEPVEAVDVRRVGKQVVARFSKREAYASLDDPRPGEKH
                     EVVIRFAMKAAEGEEPTEKELKEKVLIVGPAR"
BASE COUNT          455 a         1052 c         1072 g          432 t
ORIGIN      
        1 gaggcgatct cggaggcgat gctctgggtc gcgctggtgc agctcggtca cggcaagtac
       61 cagcggcgca tccaggccac caccaccagc aagacccaga gcatcgcctc cgagatcgcc
      121 tcggacaagc gcctcacccg ccagatcctc gaccagctcg gcctgccggt gccgcgccag
      181 gaggtggtct acgacgccgg cgaggcggtg gaggaggccg aacggatcgg ttatccggtg
      241 gtgctcaagc cgctggacgg caatcagggg cgcggcgtgt cgatcaacct caaggacgcc
      301 gagcaggtgc gggccgcgtt cggcgtcgcg cgggacatct cgcgcggcgt gatcgtcgag
      361 tcgttccagc cgggcaacga ctaccggatc ctggtggtgg acggccgggt catcgccgtg
      421 gcccagcggg tgcccggaca cgtggtgggg gacggccggc acaccatcgc cgagctggtc
      481 gagaccgtca accgcgaccc gcggcgcggc gtcggccacg agaagacgct gacccgcctc
      541 gagatcgacc atcaggcgca ccggctgctg gcgcaggccg gcctgacgct ggaatcggtg
      601 ctcccggagg gggaggtctt ctacctgcgc tcgaccggca acctgtcgac cggcggcacc
      661 tcgatcgaca agaccgacgc catccactac gacaaccgga tcatggccga gcgtggcgtc
      721 aaggcgatcg gcctcgacgt cgccggcgtc gacttcgtct cgccggacat cagccgcagc
      781 tataaagagg tgggcggcgc catcgtcgag atcaacgccg cccccggctt ccggatgcac
      841 accgcgccga ccgagggcaa ggcgcgggac gtggccggcc cggtcatcga catgctcttc
      901 cccaatggga ccccctgccg catccccatc gcggcgatca ccggcaccaa cggcaagacc
      961 acgaccaccc gcatggtggg gcacatcctc aagctcgccg gccacaccgt cggcatgacg
     1021 accaccgacg gcgtctacat tgacggggtt ctcaccgtgc gcggcgacat gaccggcccg
     1081 tggtcgagcc agctcgtgct gcgcgatccc actatcgaca cggcggtcct cgagaccgcc
     1141 cgcggcggca tcctgcggtc ggggctgggg tggaggaggt gcagcgtcgg cgcggtgctg
     1201 aacgtggccg ccgaccacct ggggctcgcc ggcatcgaca gcgtcgacga cctcgcgaag
     1261 gtcaaacagg tggtggtcga ggtggcgcag gactactgcg tgctgaacgc cgacgacccg
     1321 cgggtggcgc gcatggccga gcagagcccg gccgagccga tctacgtcac catggaccgc
     1381 accaacgagc tggtgcgcaa gcacgtgctc aaggggggca aggcggtggc gctggaggag
     1441 gggctctcgg gggcgatgct cgcgctctac cagggggagg agcagatccc gctggtgctg
     1501 gcccggcaga tcccggccac ggtcgagggg cgggcgcgcc acaacgtgca gaacgccatg
     1561 ttcgccgccg ccatcgcggt gggcatgggg gtggacctgg aggatatccg ccaggggctc
     1621 cgcaccttca gcaccgactt cttccaggcg cccggccggc tcaacttcta caacgagtac
     1681 ccgttccgcg tgctcctcga ctatgcccac aacgcccacg gcatggaagc ggtggcgcgc
     1741 atggtgcggg agctggcggt gcacgggcgc cgcatcggcg tcatcggcgc gccgggcgac
     1801 cgccgcgacg aggacatccg cgccacggcg gccgccgccg ctcccgcttt cgacctcatg
     1861 ctcctgcgcg aggacgacga cctgcggggg cgccagccgg gggaggtggc ggacctcctg
     1921 cgcgaggggc tggtgggcgc gggctttccg gcggagcgga tcatccccaa ggtccacgag
     1981 gagtcggagg cggtggagag cgccatggag atggcccgcg ccggcgacct cgtggtcatc
     2041 ttcggcgacc gggtcacccg cgactgggag ctgatcgtgg gcttcggcaa gaaggtcggg
     2101 atcaggcggc gggacgagcc cgcggtcctc atggaggcgc cggaggagcc tgtgctcgcc
     2161 ggcatgggcg gcgaggaccg ggagctgcgg gcgggctgaa gcgaagagag ggtccccccc
     2221 ggaccctctc ctcgtcaaac cactgatgcg cggctgatca gcgggcaggg cccacgatca
     2281 gcaccttttc cttcagctcc ttctccgtgg gctcctcgcc ctcggcagcc ttcatcgcga
     2341 agcggatgac gacctcgtgc ttctcgccag gccgtgggtc gtccaggctg gcgtaggcct
     2401 cccgcttgga gaagcgggcc acgacctgct tgccgacgcg gcgcacgtcc acggcctcga
     2461 ccggctcggc ctcctcgtcg gtacctacca ggacgatgga gcgcaggtcg atgtcccgcg
     2521 cgtccgagcc gcgcaggaag acgtggacct ggcccaggct ccgctcccag ttggtgttcc
     2581 aggagccggg ctggatgtcg aggtgcagct cgtcctcgcc ctcgccttcc ccctcgcccc
     2641 cgccgccgtc cggcttgccg acgatgcgga cctgatcggt cagctcctgg gcctcgtcct
     2701 cgccttcgat ttcgaaggtc agcgtgacgg tgcggcgctg gccgggcttg gcgtcctcga
     2761 acaggccgat ggcgtcggac ttgctgaacg tcgccaccac ctgaccgccg gcgaagcggg
     2821 tggagcgggg cgagatcgac ttgtcctcat cctcgctgcc cgacagctcg atgctgtcga
     2881 ggtcgatctt gccggcgtcc cggccgcgca cgaacgcctg gaggttgccc gcggcgttct
     2941 cccagttcag gttccagaca tcggggcgga cgtccagctt gagcgcgccg ccggtgttgc
     3001 ccccaccatt g
//
LOCUS       DASPJI010000004         5015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_629642, whole genome shotgun sequence.
ACCESSION   DASPJI010000004 DASPJI010000000
VERSION     DASPJI010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629642"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VEL74_00080"
     CDS             <1..570
                     /locus_tag="VEL74_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60949.1"
                     /translation="DDFETAIRWLRKGLGLIDSTRERYLELAARHSLMLYLHECGHHQ
                     EAWFLLKASRSEFLAHGGQLLSLRLLWLEGKIQHALGFLTEAEADLLAARSGFIEQGI
                     GFSAAAVSLDLATLYAGQQRAEEMRRLAEEMLLVFHSRDLHREAIGALIVFQQAVRME
                     NVDADLLTQIRSYLRRARKDHKLRFEYSA"
     gene            616..1860
                     /locus_tag="VEL74_00085"
     CDS             616..1860
                     /locus_tag="VEL74_00085"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYG60950.1"
                     /translation="MQERPDRPRLLVLGCGFGGYSLLTRLRYNRWQTTLLTPRNYFLF
                     TPLLPSAVSGSVEFRSILEPARRRLHKVQVVEGAAESVDWEAREVICTAAVGGERFSL
                     PYDYLVIAVGATVADYGVPGVQEHALKLYSVEDAQAIRRGILEQFARAEIPGLSVEQI
                     QQRLTFAVCGGGPTGVEAAAEIHDLIHEELTGSYPELAPHASVVLVEALPRILTSFDE
                     ALGDYTQVHFQREGIQVRTSSMVAAIRADGVELKDGSFLPCGLVLWAAGNAPVPFIRS
                     LGLPVTQRGGRLIVDTSLRVPGRPHVYAVGDCAAVGEPPLPATAQVAQQQGKYLAKAL
                     EWERLGEPVEPFTFQASGMLAYIGAGQALADLPQVKWSGRGAWLFWRSVYLTKLVSPA
                     NKVKVLFDWIKARLFGRDLSRF"
     gene            complement(1864..4266)
                     /gene="lptF"
                     /locus_tag="VEL74_00090"
     CDS             complement(1864..4266)
                     /gene="lptF"
                     /locus_tag="VEL74_00090"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HYG60951.1"
                     /translation="MRVRLSRYILVEILGPLGLGFLVYTFILLIRFLFTSAEMIIRRG
                     LPVPVVGKLLFLSLPNIVVLTLPMSLLFGILIAVGRLSSDSELVAMRSSGISLLTLYR
                     PILLLSAVFTALNTYLMVYALPWGNHALQQLFLDIATKTVAQQVQPRVFYEEWEGKVV
                     YVFEVPKGTNRWRGVFLAQSLPSTDKNEITVADWGEIKVDEAGERVVLLLHNAIRHQV
                     DLNAPDRYEIIRHRRLEIILEDQFTTEQKAKISVSKGIRELTLAELQDLRSDPSASEE
                     QQNLARVEIHKKFSIPMACMVFGLFALPLGFNNRRGGKASGFALSIGVIALYYILLSN
                     GEEAARFGRIPPWAAMWSPNVLLAIGGLFLLMRRNRDKSLLLSRIDRWIREDVWSGLL
                     RLDRLRRKTKEQWRQERRRREEEEDRRGRPVVRPGVVLRFQRPRILFPNLMDRYVVRL
                     FSLVFCLVLFSGLSLFVIFDLSELIDEIMKNKIGRGVVFDYYKYSALQMFYDIAPIVV
                     LVTTLMTFSLLARTNEITACKALGTSLYRLSLPAVAASLLITLFCGYIASEVLPASNE
                     KVAQLKDRIRGRETARTYRRADRQWLFGQGRYIYNYMHYDPRAQTLQRLQVFDFDGAH
                     RLSRRLYAQDARYVGDGAWMFSNGWARAFQGVEVSDFEQLEGPRVVRYPETPDYFDSE
                     IRPPEQMRYVELKKYIEDLARSGQPVPELRVQLYNKIAFPVVSLIMALVALPFAFRLG
                     RQGALYGIGLSILLGMVFLGVFAFFRTLGEAGALPPAVAVWSPGAIFGILAVYLFLGV
                     KT"
     gene            4285..4719
                     /locus_tag="VEL74_00095"
     CDS             4285..4719
                     /locus_tag="VEL74_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HYG60952.1"
                     /translation="MTLPGKVRLERRFRFSASHLYRRPDWSEEENRARFGKCANLPGH
                     GHNYRLYVTVSGEIDPHTGFVVDLGALDACVTRQVIERLDHQHLNAALPEFASGGQIP
                     SSENLIGWIHGQLVRTLPEGARLVKLRLEEDEDLAAEWSSEA"
BASE COUNT          875 a         1627 c         1718 g          795 t
ORIGIN      
        1 gacgatttcg agacggcgat ccgatggttg cgcaagggct tggggttgat cgattccacc
       61 cgggagcgct atctggagct cgcggcgcgg cacagcctga tgctctacct ccacgagtgc
      121 ggacaccacc aggaggcctg gttcctcctg aaagcttcgc ggtccgagtt cctggcgcat
      181 ggtggacagc tgctgagcct gcggctcctc tggctggagg gcaagatcca gcacgcactg
      241 ggcttcctga ccgaggccga agccgacctg ctcgcggccc gtagcggctt catcgagcag
      301 gggatcggct tcagcgccgc cgcggtgtcg ctcgacctcg cgacgctcta cgccggccag
      361 cagcgggccg aggagatgcg ccgcctcgcg gaggagatgc tcctcgtctt ccactcccgg
      421 gacctccacc gggaggcgat cggggcgctg atcgtgttcc agcaggccgt ccggatggag
      481 aacgtggacg ccgacctcct cactcagatc cgctcctact tgaggcgcgc ccgaaaggac
      541 cataagctgc gcttcgagta cagtgcctga gggggcgtgc cgctccctcg gatttgacgg
      601 gagggggagc gccctgtgca agaacgacct gatcgtccaa ggcttctggt cctcggctgt
      661 ggcttcggcg gctattcact gctcacacgg ctacgctaca accgctggca gaccactctc
      721 ctgacccccc gcaactattt cctgttcacg cccctgctcc cctcggcggt cagcgggagc
      781 gtcgagttcc gcagcatcct cgagccggcc cggcggcggc tccacaaggt ccaggtggtg
      841 gagggcgccg cggagtccgt cgattgggag gcgcgggagg tcatctgcac ggcggcggtc
      901 ggtggagaga ggttctccct gccgtatgac tacctggtca tcgccgtcgg ggcgaccgtc
      961 gccgactacg gagtgcccgg ggtccaggag catgccctga agctgtacag cgtcgaggac
     1021 gcccaggcga tccggcgggg aattctggag cagttcgcgc gggccgagat cccggggctg
     1081 agcgtggagc agatccagca gcgcctgacc ttcgcggtct gcgggggggg gcccaccggg
     1141 gtcgaagccg cggccgagat ccacgacctc atccatgagg agctcaccgg cagctacccg
     1201 gagctcgctc cccatgcctc ggtggtcctg gtggaggcgc tcccgcgcat tctcaccagc
     1261 ttcgacgaag cgctgggcga ttacacccag gtccacttcc agcgcgaggg tatccaggtc
     1321 cgcacctcgt ccatggtcgc cgcgatccgc gccgacgggg tggagctcaa ggacggctcc
     1381 tttctcccgt gtggcctggt cctgtgggcg gccgggaacg ctccggtgcc gttcattcgg
     1441 tctctcggcc tgcccgtcac gcaacgtggc ggccggctga tcgtcgacac ctctctccgg
     1501 gtgcccggcc ggccgcacgt ctacgcggtc ggggattgcg ccgcggtggg ggagccgccg
     1561 ctgccggcca ccgcgcaggt ggcgcagcag cagggcaagt atctggccaa ggcgctggag
     1621 tgggaacggc tgggagagcc ggtcgagccg ttcaccttcc aggcctccgg tatgctcgcc
     1681 tacatcggag ccggccaggc gctggccgac ctgccgcagg tcaaatggag cgggcgcggc
     1741 gcctggctct tctggcggtc ggtctacctg accaaactgg tcagccccgc caacaaggtg
     1801 aaggtcctct tcgactggat caaggcgagg ctcttcggcc gggacctcag ccgcttctga
     1861 gcctcacgtc ttcaccccca ggaagagata gacggccagg atcccgaaga tcgcccccgg
     1921 gctccagacc gccaccgcgg gcgggagcgc ccccgcttcg cccagcgtcc tgaaaaaggc
     1981 gaatacgccc aggaagacca tcccgaggag gatggagagg ccgatgccgt agagcgcgcc
     2041 ctgccggccc agccggaacg cgaaggggag ggcgacgagc gccatgatca gcgagaccac
     2101 ggggaaggcg atcttgttgt agagctgcac ccgtagctcc gggaccggtt ggccgctcct
     2161 cgccagatcc tcgatgtact tcttgagctc cacatagcgc atctgctccg gaggccggat
     2221 ctccgagtcg aagtagtccg gggtctcggg atagcgcacg accctgggcc cttccaactg
     2281 ctcgaagtcg ctcacctcga caccctggaa ggcgcgcgcc cagccgttcg agaacatcca
     2341 cgcgccgtcg ccgacgtagc gggcgtcctg ggcatagagc cgccgtgaca gccggtgcgc
     2401 cccgtcgaaa tcgaacacct gcagacgctg gagggtctgt gcccgggggt cgtaatgcat
     2461 gtagttgtag atgtagcggc cctggccgaa cagccactgg cggtcggccc tgcgataggt
     2521 acgggcggtc tcgcggccgc ggatgcggtc cttgagctga gcgaccttct cgttggacgc
     2581 cggcaggacc tccgaggcga tgtagccaca gaacagggtg atcagcagcg acgccgccac
     2641 ggcgggcagg gagagccggt agaggctggt gccgagcgcc ttgcaagccg tgatctcatt
     2701 ggtgcgcgcc aggaggctga aggtcatcag ggtggtgacc aggacgacga tgggcgcgat
     2761 gtcatagaac atctgcaggg ccgagtactt gtagtagtcg aagacgaccc ctcgcccgat
     2821 cttgttcttc atgatctcgt cgatgagctc ggagaggtcg aagatcacga agagcgacag
     2881 ccccgagaag agcaccaggc agaacaccag ggagaacagg cggaccacat accggtccat
     2941 caggttgggg aagaggatcc tgggcctctg gaaccggagg acgactccgg gacgcaccac
     3001 cggcctgccg cgccggtcct cctcctcctc gcgccggcgg cgctcttgac gccattgctc
     3061 cttggtcttc ctcctcaggc ggtcgaggcg gagaagccca ctccagacgt cttcccggat
     3121 ccagcggtcg atgcgcgaga ggaggaggct cttgtcccga ttccgtctca tgaggaggaa
     3181 cagcccgccg atcgccagca gcacgttggg agaccacatg gccgcccagg gggggatccg
     3241 gccgaaccgc gccgcctcct cgccgttgct gaggaggatg tagtagaggg cgatgactcc
     3301 gatcgaaagg gcaaatcccg aggccttgcc gccgcggcgg ttgttgaatc cgaggggcag
     3361 ggcgaagagg ccgaacacca tgcaggccat ggggatcgag aacttcttgt ggatctccac
     3421 ccgcgccagg ttctgctgct cctcggaggc ggatggatcg gagcgcaggt cctggagctc
     3481 ggcgagggtc agctcccgga tccccttgga caccgagatc ttcgccttct gctcggtggt
     3541 gaactgatcc tcgaggatga tctcgaggcg ccggtgccgg atgatctcgt agcggtcggg
     3601 ggcgttcagg tccacctgat gccggatagc gttgtggagc agcaggacga cccgttcacc
     3661 cgcctcgtcc actttgatct ccccccagtc ggcgacggtg atctcgttct tgtcggtgga
     3721 ggggagagac tgggcgagga agacgccccg ccagcggttc gtccccttcg ggacctcgaa
     3781 gacgtagacg accttgcctt cccactcctc atagaacacc cgcggctgga cctgctgtgc
     3841 cacggtcttg gtcgcgatgt ccagaaagag ctgctgcagg gcgtggttac cccagggcag
     3901 cgcgtagacc atgaggtacg tgttgagggc cgtgaacacg gccgagagca gcaggatcgg
     3961 ccggtacagg gtgagcaggc tgatcccgga ggacctcatg gccacgagct cgctgtccga
     4021 ggagagccgc ccgacggcga tgaggatgcc gaacagcagg gacatcggca gcgtcaggac
     4081 cacgatgttc ggcagcgaca ggaacagcag cttgccgacc accggcaccg gcaggccgcg
     4141 ccggatgatc atctcggccg acgtaaacag gaaccggatg aggaggatga aggtatagac
     4201 taggaagccc agcccgagcg ggcccaggat ctcgacgagg atatagcggc tcagtctcac
     4261 acgcatcgga cgcattatat ggagatgacc ctgccaggga aggtccggct cgaacgccgg
     4321 ttccgtttca gcgccagcca cctctaccgg cgccccgact ggagcgagga ggagaaccgg
     4381 gcccgcttcg ggaagtgcgc caatctgccg ggccacggcc acaactaccg gctctatgtg
     4441 acggtgagcg gcgagatcga cccgcacacc ggcttcgtcg tggacctcgg ggcgctcgac
     4501 gcctgcgtca cccggcaggt catcgagcgc ctggaccacc agcacctcaa cgccgcgctg
     4561 cccgagttcg cgagcggcgg gcagatccca tccagcgaga acctgatcgg ctggatccat
     4621 ggccagctcg tgcgcaccct tccggaaggg gcccggctgg tgaagctccg gctcgaagag
     4681 gacgaggatc tggcggcgga gtggagctcg gaagcctgag atccaggccg ggtcgcagcc
     4741 ggtcgccgca ggatggttaa cataatgcgc cttatgcgac tgacgacagc cggaagtttc
     4801 cggcggctcc tcggaggccc ggcagcccgc cggaagcttc cgagggcttg ccgaagagtc
     4861 cgtcgatgcg tcgaagacat cgatgagcca cgggagatta cggcggctgg cgggaagctc
     4921 cgacgggcca ccgaagactt ctccaaggcg ccgcaacctg tgacggtgcg ccgaaggctt
     4981 cggtagcctg ccggaagctc cgaggacgcg cccga
//
LOCUS       DASPJI010000005        15257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_229289, whole genome shotgun sequence.
ACCESSION   DASPJI010000005 DASPJI010000000
VERSION     DASPJI010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15257)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15257
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229289"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="VEL74_00100"
     CDS             <1..1261
                     /locus_tag="VEL74_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60953.1"
                     /translation="TPTPDGSGLFYLSLEPDGLDLFRLDLAAPPSAIAAADLPALPAA
                     ELAPAIPPPPPAPPAPFSLAEVPAGRPYGLGRQEISAHLGGSSTSSGSAWELGARGGD
                     LVGRLGYLVFGSLSNGGWPEGGAVAAAWRGWPVTVGLHAFVAKERPSEQADEWPRSPD
                     SLELVDARVLDVDRQGIELSAGWDRLWSGWSQNRLELGGRLLAGDVDPAAGPALSQEI
                     GALSAGLSGRTSRGQWRLLHGAVLQYEEGRTGGDGWSRYGGGLRLGLGYDDTALRVTW
                     RRDGSRDVVRGFDLYQLGGAESSLLPESVLSGRITVPALPVGTFLGEEHEGQRAELTL
                     GFLPGPLFYERHRLWSGAGDSERPDEWLSLAGLEWQWSLDPIPIGRIPALDVRAGVAR
                     ILDVGVEGAGEPLEGDTRWWLTAVWRP"
     gene            1274..2803
                     /locus_tag="VEL74_00105"
     CDS             1274..2803
                     /locus_tag="VEL74_00105"
                     /inference="COORDINATES: protein motif:HMM:NF014610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HYG60954.1"
                     /translation="MADRLFIIRLAGDFYTKSRGTRLQFQRRLASNVRDALRASGIPH
                     RLERTWSRFYLETEDAGASEVLTRVFGVQSVSEVERRPWSTLDDVVRAGVEVFAGKVQ
                     GRSFAVRASRRSKNGQREVFGFDSQAVEIALGRALLDAGAGRVDLGAPEVTAYVELEP
                     GGAHLFEDKARGHGGLPIGVEGRALALVSGGFDSAVASWILLKRGVQLDYLFCNLGGT
                     AHRLGVLKVLEVLATRWSYGSRPRLHEVDFQPLAAEIQAGTNPRNWQILLKRQMVRAG
                     EIIGRRTGRLALITGDAIGQVSSQTLQNLAVVSQAASALPILRPLLGFNKEEILALAR
                     QIGIYELSAAVGEYCALVPRKPATRAPLKETLAEESVLDLGRLDELIAARQTYELRRL
                     APELLAPPELEIGHVPEGATVLDLRPKSAWQAWHYPDSLRLDFPQALQTFGSFDRERT
                     YVLVCEVGLKSAHLAEEMRKGGFRAYHFAGGAGELMELEARKRRLSGLERALLAPAVR
                     D"
     gene            complement(3274..3504)
                     /locus_tag="VEL74_00110"
     CDS             complement(3274..3504)
                     /locus_tag="VEL74_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60955.1"
                     /translation="MVISSVTRFHSQVTTPETRCASSSRALLSRSATSASLRSVMSTA
                     VVKAVVSPAPSITAEVSSHQRSRPSRVRNLTS"
     gene            complement(3511..3780)
                     /locus_tag="VEL74_00115"
     CDS             complement(3511..3780)
                     /locus_tag="VEL74_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60956.1"
                     /translation="MRSLTSWATPISRTGAPVSSRRARPRSASQTSRPSGATTRSSTL
                     KSWPLRRQSWSARTRSGRSSSQTAAISSLPVAQTASGAQPRTSAA"
     gene            4077..5954
                     /locus_tag="VEL74_00120"
     CDS             4077..5954
                     /locus_tag="VEL74_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYG60957.1"
                     /translation="MRSAGIESMVAVPLFSSGGRPLGHIGVMDVKPLPPGLPALSILK
                     IFAARVAAEVERTLAEEALAQEKERAQVTLASIGDGVIRTDAAGRIDYLNPVAERLTG
                     WTAEEAFQQPVMEVFRVVEEGTGKPLPNPVERCLREGRVIELPGASVLTPRHGGELAI
                     RDSVAPIRDRQGRITGSVLVFKDVTQLRGLEREMLFLARHDPLTGLLNRREIEKRLQA
                     CLDTARDEGRQHAFFYFDLDEFKVVNDICGHVAGDEMLKQVAALLQSRLRTADSLARL
                     GGDEFGLLLEDTTLEKARSVGGELQQALRGFRFAWQERIFETGASIGLVPLTRGCGDL
                     AQVLSAADAACYVAKEAGRNRLHEYQTDDTALAERYGEMQWIHRIHQAFAEHRFCLYH
                     QSIEPLGPPADGALDEPPFFEIFIRLREEGGRIVTPGSFIPAAERYHLIPSIDRWVVH
                     EALSALAARAVRGQRGDTTTRFAINISGQSLGDESFLDHVLAEIEATGAAPGRVCFEI
                     TETAAVGNLAQAMRFIAALKALGCCFVLDDFGSGLSSFAYLKNLPVDFLKIDGAFVRD
                     MIGNSVQRALVESIHQIGRVMGIRTIAESVEDRETLDALRRIGVDYAQGYVLSIPSPL
                     D"
     gene            6021..8138
                     /locus_tag="VEL74_00125"
     CDS             6021..8138
                     /locus_tag="VEL74_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYG60958.1"
                     /translation="MALLSTASILPLLSGSETGSADPGDDSQSLLHADDGLQLSAGQM
                     PPILWTTDRDLRFGLGVAPGLRNRRSSGSLVDYIQGCDPDSVPTRAHRLALEGEPVSF
                     DQEWNGRHFQIRVEPLRDAAGQITGCMGVAVDITERKHSEEALYREKERAQVTLASIG
                     DGVIRTDAAGLIDYLNPVAERLTGWSMEDALGRPVTEVFQVVDEVTRQPVADPVQRCL
                     TEGRAVESPGYTVLLSAGGKEYSVRDSSAPIYDRSGAAMGAVLVFKDVTQIRGMEREM
                     IYLASHDPLTGLINRREFEIRLKRAIRNARAERRQHVLLYLDLDEFKVVNDTCGHLVG
                     DEMLRQITALLRSRVRRSDVLARLGGDEFGVLLEDCPLDHARQIAEEMRRTVREFRFC
                     WKDQIFEVGVSIGLVPINHDSGDLASVMSAADAACYVAKDGGRNRVHEYELDDTAVAE
                     RYGEMQWIHRIHAAFDDRRFRLFYQLIQPLAAENSSRELLCEVFVRMLDRNGKIIEPS
                     AFIAAAERYHLMSNLDRWVIKTAFKALAEAQRREVSRPVLFAINLSGQSLSDDSFLAF
                     VVEELENSSVDPRRICFEITETAAISKLDGAMHFIGALKAKGCRFILDDFGSGLSSFA
                     YLRDLAVDFLKIDGEFVQNMLEDRVKRAMVESINQIGHVMGLQTIAEWVESRQTLEAL
                     RDLGVDYAQGYWLCHPQPLVHDV"
     gene            complement(8145..10004)
                     /locus_tag="VEL74_00130"
     CDS             complement(8145..10004)
                     /locus_tag="VEL74_00130"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HYG60959.1"
                     /translation="MDAFAEQALDLLARASLQGAVLIGLVWAVCRLLPRLPASLRCAL
                     WWLACLQLLVGLVWTEPVALPLLPARETMTAAPAAPVISSSVPVWETVSAPVAPAAPA
                     APSPAAGFPWGWAALGVWLTGLGLHLALALAQLRETRRVVRGAEPVAVPWMRQAFAEM
                     VRLLGLRRSPDLRSSTAIATPQALGLLRPLVLLPRDAMERLSPGEVTMTLCHEALHVR
                     RGDLWLGWVPALAERLFFFHPLAALAAREYALAREAACDAEVLRLLGAAPQDYGRLLV
                     RLGVSPRQAGLAAAGASPSFLNLKRRLQMLQNSSERSRRSGAWWIVPVVLLAGVVPYR
                     IVAAEAEGPRAAKPVAATKAAPAPKAAPAVAARPAVAPKAAPKAVPAVAARPAMAPKA
                     APAAQRGNRTGPPPAPPAPPAPPAPPAPPAPADGKGHRHVYRNGDGGDDYFLRLGDSV
                     TMNGSTSDREMEEALERDGDVLWFRQGGKAYVIRDAAFLRRAGDLFERQKELGQKQAE
                     LGRKQAELGRKQAELGRQQAALGREQGALARQEARRDYDPEETERKRRELGEKQGALG
                     GQQGELGARQGELGEQQGELGRQQAEEGEKAERALRVLMAEAVRNGTAQEVRK"
     gene            complement(10006..10392)
                     /locus_tag="VEL74_00135"
     CDS             complement(10006..10392)
                     /locus_tag="VEL74_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HYG60960.1"
                     /translation="MTSKSIGDQELALLRYVAEQGPVSVGEAVEGFGTARGLARSTVL
                     TMMERLRQKGHLEREPVDGVYRYASTASPGELMRGAVRSFVEKTLAGSVSPFVNYLAE
                     TGDVSAEELAELEDLVNRLKSRKKES"
     gene            complement(10877..11167)
                     /locus_tag="VEL74_00140"
     CDS             complement(10877..11167)
                     /locus_tag="VEL74_00140"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYG60961.1"
                     /translation="MRLVLRRAAKLDLVEAYRWYEEQRPGLGESFRAEVKAVLATLQA
                     HPQLFPRVGPRIRRATTGQFPYGIFYLIDGETIRVLAILHRARDPEIWRSRR"
     gene            complement(11164..11391)
                     /locus_tag="VEL74_00145"
     CDS             complement(11164..11391)
                     /locus_tag="VEL74_00145"
                     /inference="COORDINATES: protein motif:HMM:NF021253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HYG60962.1"
                     /translation="MTRADLQKLLDLPAEERREIAQVLWESVEPKDEARFVSIPDWQR
                     RILDERLADLERNPHDEQTWEEVEAELWPDR"
     gene            complement(11414..11917)
                     /locus_tag="VEL74_00150"
     CDS             complement(11414..11917)
                     /locus_tag="VEL74_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015859633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HYG60963.1"
                     /translation="MELIVRDGEREERVRVRRDGDVYEVTLGDRTWRVEAATVGKSVH
                     SLRLDGAHHEVAVRPQADGVYWVSTPQGTSPVTVADPLTHLAAQAAGGKGGRRQQKVT
                     AYMPGRVVAVLVEEGQEVQAGQGVVVLEAMKMENEIRAEHEGTIGHVFVTPGQAVEGG
                     DPLFELV"
     gene            complement(11908..13476)
                     /locus_tag="VEL74_00155"
     CDS             complement(11908..13476)
                     /locus_tag="VEL74_00155"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HYG60964.1"
                     /translation="MPATSPDVPKKRIRKVLIANRGEIAVRILRACRELGIRSAVIYS
                     EPDRLGLPVLLADEAYPIGPAPSRESYLRAEAIVELAVRIGADAIHPGYGFLSEREAF
                     SRLCRDAGVTFIGPSPEAIAAMGSKVESRRLMMAAGVPVVPGGKDPLPDLESAAAAAE
                     HIGYPVMLKAAAGGGGKGMRMIPSAAELPAAYRAARSEAGASFGDDSVYLEKYIVNPR
                     HVEIQVMGDLHGKVVSLGERECSLQRRHQKVVEEAPSPVVTPELRARMGAAAVRAAQA
                     VGYSNAGTCEFLLSADGEFYFLEMNTRLQVEHPVTELVTGIDLVVTQIRVAEGEPLGP
                     EFDALQSHGPRGHAIEVRLYAEDPYQRFAPSPGRIELLRWPDGPGVRVDAGVYEGSEV
                     SIYYDPMLAKLIVWAADRKLALARLGRALAELRVEGIRTTVPLYRALLADPDFVSGNM
                     DIGMLDRKLAAGELQPPPGDEPADLPLIAAAVAHFERVGRSLADPAASGGQSRRSRWG
                     LAARREALREGAWS"
     gene            complement(13490..>15257)
                     /locus_tag="VEL74_00160"
     CDS             complement(13490..>15257)
                     /locus_tag="VEL74_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60965.1"
                     /translation="GWRRLALLAGCFTAGTLLALPQLVEFLRILPLSFRGQKGYEPQV
                     ATLASWDPYQVAEWFLPMIFGRLDMLSLASFWGKKFFTGVPPYYVTLYPGLLTLALVL
                     ASGRPRRRAAWWAWGAVVFGLFFSLGRFNPAAEWVFTLRNGALRYPVKLWLPLAVGAA
                     LLCGLCFQRLLGPADDPLTRRARRRFGLALLGLFLVLGGFWAFLTFQPGTAEELASRI
                     IPKAFQTEQPFVRNERLRWAGLCLFSFAVLAGLAAGARLLRRRPALGGAVILAVHAVS
                     QVLLLRPAYPTDPIQPYLIPPPALDYVPADVRVVNPDLNSLFGKHTLRDGKFPERKGN
                     WLERRAFYELYSFTGPQWGRRYDLNVSPEGLDTYLARMAQGAVRGSNDTQRIRMLAAW
                     GVGRLLMNHPLTPDTPGVRELAAIPSFGFHLYIYEVLDRAPEVFLAHRAVFAPHMNAA
                     YRTLSGAMGGVSSFNARRDVVLPGNGKPEALGGGTVRIVKDEPEELEVEADVGPGGSV
                     LVVQRSALLQKATVDGRPAEVLNANLQRAGIRVPAGRHRVRLWIDRRPFELSLGGTAL
                     GLLLLPVLFWWGRRPAGRDPLL"
BASE COUNT         2168 a         5604 c         5379 g         2106 t
ORIGIN      
        1 cacccccacg cccgacggct ccggcctctt ttatctgagc ctggagcccg acggcctcga
       61 cctgttccgg ctcgacctcg cggccccccc ttcggccatc gcggcggcgg acctcccggc
      121 gctgccggcc gcggagctgg cgccggccat cccgccgcct ccgccggcgc cgcccgcacc
      181 cttcagcctc gccgaggtcc ccgccggccg cccgtacggg ctgggacggc aggagatctc
      241 ggcccacctg ggcggtagct ccacctcgtc cggcagcgcc tgggagctgg gggcgcgcgg
      301 cggcgacctg gtggggcgcc tcggctacct ggtcttcggc tccctgtcga acggcggctg
      361 gccggagggc ggtgcggtgg ccgcggcctg gcgcggctgg ccggtgaccg tcggcctgca
      421 cgccttcgtc gccaaggagc gcccgtcgga gcaggcggac gaatggccgc gctcgcccga
      481 ctccttggag ctcgtcgacg cgcgggtcct tgacgtggac cgccagggga tcgagctgtc
      541 ggccggctgg gaccggctgt ggagcggctg gagccagaac cggctcgagc tcggcggccg
      601 gctgctggcg ggcgacgtcg atccggccgc cggccccgcg ctctcgcagg agatcggcgc
      661 gctctcggcc ggcctctccg gccgcacctc ccgcggccag tggcgcctcc tgcacggcgc
      721 cgtcctccag tacgaggagg ggcgcaccgg cggcgacggc tggtcccgct acggcggcgg
      781 cctgcgcctc ggcctcggct acgacgacac ggcgctgcgc gtcacctggc ggcgcgacgg
      841 ctcgcgcgac gtggtccgcg gcttcgacct ctaccagctc ggcggcgcgg agagcagcct
      901 gctgccggag tcggtgctga gcggccggat cacggtgccg gccctgccgg tgggcacctt
      961 cctcggtgag gagcacgagg ggcagcgggc cgagctgacc ctgggcttcc tccccgggcc
     1021 gctcttctac gagcgccacc gcctctggtc cggcgccggc gacagcgagc ggcccgacga
     1081 gtggctgtcg ctcgccggcc tcgaatggca gtggtccctc gatcccatcc ccatcgggcg
     1141 gatcccggcg ctggacgtgc gcgccggcgt cgcccgcatc ctcgatgtcg gcgtcgaggg
     1201 tgccggcgag ccgctggaag gcgacacccg ctggtggctc accgcggtct ggcgtcccta
     1261 ggagagagat ggcgtggcgg accgtctgtt catcatccgg ctggccggcg acttctacac
     1321 caagtcgcgg ggcacccgcc tgcagttcca gcgccggctg gcctccaacg tccgcgacgc
     1381 cctcagggcc agcggcatcc cccaccggct ggagcgcacc tggagccgct tctacctgga
     1441 gaccgaggac gccggcgcgt ccgaggtcct gacccgcgtc ttcggcgtgc agtcggtctc
     1501 cgaagtggag cgccgcccgt ggagcaccct ggacgacgtg gtgcgggccg gcgtcgaggt
     1561 gttcgccggc aaagtacagg gccgcagctt cgccgtccgc gccagccgcc ggtcgaagaa
     1621 tgggcaacgg gaggtcttcg gcttcgactc ccaggcggtg gagatcgccc tcggccgcgc
     1681 cctgctggac gccggcgccg gccgcgtcga cctcggcgcg ccggaggtca ccgcctacgt
     1741 cgagctggag cccggcggcg cccacctgtt cgaggacaag gctcgcggcc acggcggcct
     1801 gcccatcggc gtcgaggggc gtgccctggc gctggtctcc ggcggcttcg actcggcggt
     1861 ggcgagctgg atcctgctca agcgcggcgt ccagctcgac tacctcttct gcaacctcgg
     1921 cgggacggcg caccggctgg gggtgctcaa ggtgctcgag gtcctggcca cgcgctggag
     1981 ctacggctcc cggccgcgcc tgcacgaggt cgacttccag ccgctggccg ccgagatcca
     2041 ggccgggacc aacccgcgca actggcagat cctgttgaaa cggcagatgg tgcgcgccgg
     2101 cgagatcatc ggccgccgca ccgggcggct ggcgctgatc accggcgatg ccatcggcca
     2161 ggtctcctcg cagacgctgc agaacctggc ggtggtctcc caggccgcct cggccctgcc
     2221 gatcctccgc ccgctgctcg gcttcaacaa ggaggagatc ctcgccctgg cgcgccagat
     2281 cggcatctac gagctgtcgg cggcggtcgg cgagtactgc gccctggtgc cacggaagcc
     2341 ggcgacccgg gcgccgctca aggagacgct ggccgaggag tcagtcctcg acctcggccg
     2401 gctcgacgag ctcatcgccg cacgccagac ctacgagctg cggcggctcg ctccggagct
     2461 cctggcgccg ccggagctgg agatcggcca cgtaccggaa ggcgccacgg tgctcgacct
     2521 gcggccgaag agcgcctggc aggcctggca ctacccggac tccctgcgcc tcgacttccc
     2581 gcaggcgctc cagaccttcg gctccttcga ccgggagcgc acctacgtgc tggtctgcga
     2641 ggtgggcctc aagagcgccc acttggccga ggagatgcgc aagggcggct tccgcgccta
     2701 ccacttcgcg ggcggcgccg gcgagctgat ggagctggag gcccggaagc gccgcctgag
     2761 cggcctggag cgggccctgc tcgcgcccgc ggtgcgcgat tagacccgcc gctccggcgc
     2821 catcccccgc cccgccggag ccgctcccgc gcactccgga gataagcccg gagatcggcc
     2881 cggagggcaa gaagatgccg cttcggaaga gtgcaagaat ttgcttgcat ctcaatgcgc
     2941 caactcttat tctcgatgcc cttcaattcg cggcatggca gagagcaaaa acccggtgac
     3001 acgcaccgcc cggagagccg cccggcctga ccggcaacca ccgcccacca ccgagcagtg
     3061 gttccgcacg ctggccgaga cgacctacac cgccatcttc gcctatcggg cggaccggct
     3121 gatctacatc aacccggcgg cggaggcgct gaccggctac ccggcggcgg aggtcctcgg
     3181 caagcccccc tgggagctcg cccatcccga ccaccaggcg atggtgcgcg aacggacgat
     3241 ggcgcggctg cgcggcgagc cggcgccggt ccactacgaa gtcaagttcc tcacccgcga
     3301 cggccgcgag cgctggtgcg agctgacctc ggcggtgatg gagggcgccg gcgagacgac
     3361 ggccttgacc accgccgtgg acatcaccga gcgcaagctc gccgaggtgg cgctgcgcga
     3421 gagcagcgcc cggctcgagc tggcgcagcg cgtctccggc gtcgtcacct gggagtggaa
     3481 cctggtgacc gacgagatga ccatctcgcc ctacgccgcc gaggtcctcg gctgcgcgcc
     3541 ggacgcggtc tgggcgaccg gcagggagct gatcgccgcg gtctgcgaag aggaccggcc
     3601 ggacctggtg cgggcgctcc agctctgcct gcgcaggggc caggacttca gcgtcgagct
     3661 gcgcgtcgtg gcgcccgacg gccggctggt ctggctggcg gagcgcgggc gcgccctgcg
     3721 cgacgagacc ggcgcgccgg tacggctgat cggcgtcgcc caggacgtca gcgagcgcaa
     3781 gcgggccgag gagcggctgc gctcgatcgt cgaggggacc tcctccagcg tcggcgccga
     3841 cttcctgcgc ggcctggtgc gccacctggc cgaggcgctg ggcgtgcgct acaccttcgt
     3901 ctccgaggtg gcggacggcg cgctcaaccg cgcccgcttc atcgccgtct gggcgggcga
     3961 gagccacggc gagccggtgg agttggggct cgaggggacg ccgctcgaga acgtgctcgc
     4021 cggggatctc tgctactacc ccgcgggcat cctcgacctt ccctcacgac ccctggatgc
     4081 gctcggcggg catcgagagc atggtcgcgg tgccgctctt ctcctcgggc ggccgccccc
     4141 tcggccacat cggcgtcatg gacgtcaagc cgctgccccc cggtctgccg gccctctcca
     4201 tcctcaagat cttcgccgcc cgcgtcgccg ccgaggtgga gcggacgctc gccgaggagg
     4261 cgctggcgca ggagaaggag cgggcccagg tgaccctcgc ctcgatcggc gacggcgtca
     4321 tccgtaccga cgcggccggc cgcatcgact acctcaaccc ggtggccgag cggctcaccg
     4381 gctggacggc cgaggaggcc ttccagcagc cggtgatgga ggtcttccgg gtcgtcgagg
     4441 aggggaccgg caagccgctg cccaacccgg tggagcgctg cctgcgcgag gggcgcgtca
     4501 tcgagctgcc cggcgcgtcg gtcctcaccc cgcgccacgg cggcgagctc gccatccgcg
     4561 actcggtggc gccgatccgc gaccgccagg gccggatcac cggcagcgtg ctggtgttca
     4621 aggacgtgac ccagctccgc ggcctggagc gcgagatgct gttcctggcg cggcacgacc
     4681 cgctcaccgg cctgctcaac cggcgcgaga tcgagaagcg cctccaggcc tgcctcgaca
     4741 ccgcccgcga cgagggacgg cagcacgcct tcttctactt cgacctcgac gagttcaagg
     4801 tggtcaacga catctgcggc cacgtggcgg gcgacgagat gctcaagcag gtggcggcgc
     4861 tcctccagtc ccggctgcgg acggccgact ccctggcgcg gctgggcggc gacgagttcg
     4921 gcctgctgct cgaggacacg acgctggaga aagcgcggag cgtcggcggc gagctccagc
     4981 aggccctccg cggcttccgg ttcgcctggc aggagcggat cttcgagacc ggcgccagca
     5041 tcggcctggt ccccctcacc cgcggctgcg gcgacctcgc ccaggtgctg tcggcggccg
     5101 acgccgcctg ctacgtcgcc aaggaggcgg gccgcaaccg cctccacgag taccagaccg
     5161 acgacaccgc cctcgccgag cgctacggcg agatgcagtg gatccaccgc atccaccagg
     5221 ccttcgccga gcaccgcttc tgcctctatc accagagcat cgagccgctc ggcccccctg
     5281 cggacggcgc cctggacgag ccgccgttct tcgagatctt catccgcctg cgcgaggagg
     5341 ggggccggat cgtcacgccg ggctccttca tcccggcggc cgagcgctac cacctgatcc
     5401 cgtcgatcga ccgctgggtg gtgcacgagg cgctgtcggc cctcgccgcc cgcgctgtcc
     5461 ggggccagcg gggggacacc accacccgct tcgccatcaa catttcgggg cagtcgctgg
     5521 gcgacgagag cttcctcgac cacgtgctgg cggagatcga ggcgaccggc gccgcccccg
     5581 gccgcgtctg cttcgagatc accgagaccg ccgcggtcgg caacctggcg caggcgatgc
     5641 gcttcatcgc cgcgctcaag gcgctgggct gctgcttcgt gctcgacgac ttcggcagcg
     5701 gcctctcctc cttcgcctat ctgaagaacc tgccggtcga cttcctgaag atcgacggcg
     5761 ccttcgtgcg cgacatgatc ggcaactcgg tgcagcgggc gctcgtcgag tcgatccacc
     5821 agatcggccg ggtgatgggc atccgcacca tcgccgagtc ggtggaggac cgggagaccc
     5881 tggacgccct gcgccggatc ggcgtcgact acgcccaggg ctacgtcctc tccatccctt
     5941 cgccgctcga ctaggcggcg ggcctgacag ctcgcccggc gcggttcttg ctactgtgcc
     6001 agccgggagc tacgtctacc atggcgctac tttctacagc cagcatcctt cctctcctct
     6061 cggggagcga gaccggttcc gcggaccccg gcgacgactc gcagtccctg ctccacgccg
     6121 acgacggcct gcagctctcg gccggccaga tgccgccgat cctgtggacc accgaccgcg
     6181 acctccgttt cggcctcggc gtcgcccccg gcctgcgcaa ccggcgctcc tcgggcagcc
     6241 tggtggacta catccagggc tgcgacccgg actccgtgcc gacgcgcgcc caccggctgg
     6301 ccctcgaagg cgagccggtg agcttcgatc aggagtggaa cggccggcac ttccagatcc
     6361 gcgtcgagcc gctgcgcgac gccgccggcc agatcaccgg ctgcatgggc gtggcggtgg
     6421 acatcaccga gcgcaagcac tccgaggagg ccctgtaccg ggagaaggag cgcgcgcagg
     6481 tgaccctcgc ctcgatcggc gacggcgtca tccgcaccga cgccgcgggg ctcatcgact
     6541 acctcaaccc ggtggccgag cggctcaccg gctggagcat ggaagacgcc ctcgggcggc
     6601 cggtgaccga ggtcttccag gtggtggacg aggtgacgcg ccagccggtc gccgaccccg
     6661 tgcagcgctg cctgaccgag gggcgggccg tggagtcgcc cggctacacc gtgctgctga
     6721 gcgccggcgg caaggagtac tcggtgcgcg actcgtcggc gccgatctac gaccggagcg
     6781 gcgccgccat gggggccgtc ctggtgttca aggacgtgac ccagatccgc ggcatggagc
     6841 gcgagatgat ctatctcgcg agccacgacc cgctcaccgg cctgatcaac cgccgcgagt
     6901 tcgagatccg cctgaagcgc gccatccgca acgcccgcgc cgagcggcgc cagcacgtgc
     6961 tgctctacct ggacctcgac gagttcaagg tggtcaacga cacctgcggc catctggtgg
     7021 gcgatgagat gctgcgccag atcacggcgc tgctgcgctc gcgggtgcgc cgcagcgacg
     7081 tcctcgcccg cctgggcggc gacgagttcg gcgtcctcct cgaggactgc ccgctcgacc
     7141 acgcacgcca gatcgccgag gagatgcggc gcaccgtgcg cgagttccgg ttctgctgga
     7201 aggaccagat cttcgaggtc ggggtgagca tcggcctggt gccgatcaac cacgacagcg
     7261 gcgacctggc gagcgtcatg agcgccgccg acgccgcctg ctacgtcgcc aaggacggcg
     7321 gccgcaaccg cgtgcacgag tacgagctgg acgacaccgc ggtggccgag cgctacggcg
     7381 agatgcagtg gatccaccgc atccacgccg ccttcgacga ccggcggttc cgtctcttct
     7441 accagctcat ccagccgctg gcggcggaga actcgagccg ggagctgctg tgcgaggtct
     7501 tcgtccgcat gctcgaccgc aacggcaaga tcatcgagcc gtcggccttc atcgccgcgg
     7561 ccgagcgcta ccacctgatg tccaacctcg accgctgggt gatcaagacc gccttcaagg
     7621 cgctggccga ggcgcagcgg cgcgaggtct cgcgtccggt cctcttcgcc atcaacctgt
     7681 cgggccagtc gctgtcggac gacagcttcc tcgccttcgt cgtcgaggag ctggagaaca
     7741 gcagcgtcga cccgcgccgg atctgcttcg agatcaccga gaccgcggcg atcagcaagc
     7801 tcgacggcgc catgcacttc atcggcgcgc tcaaggccaa ggggtgccgc ttcatcctgg
     7861 acgacttcgg cagcggcctg tcgtcattcg cctacctgcg cgacttggcg gtggacttcc
     7921 tgaagatcga cggcgagttc gtgcagaaca tgctggagga ccgggtgaag cgggcgatgg
     7981 tcgagtcgat caaccagatc ggccacgtca tggggctgca gacgatcgcc gagtgggtgg
     8041 agagccggca gacgctcgag gcgttgaggg atctgggggt ggattacgcg cagggctact
     8101 ggctctgcca cccccagccc ctcgtgcacg acgtctagat ccgcttactt ccggacctcc
     8161 tgggccgtcc cgttgcggac ggcctcggcc atgagaacgc ggagggcccg ctcggccttc
     8221 tccccctcct cggcctgctg gcggcccagc tcgccctgct gctcgccgag ctccccctgg
     8281 cgcgcgccga gctcgccctg ctgcccgccg agggctccct gcttctcccc cagctcccgc
     8341 cgcttccgct cggtctcctc gggatcgtag tcgcgccgcg cctcctgccg tgccaacgcc
     8401 ccctgctcgc ggccgagggc agcctgctgg cggccgagct ccgcctgctt ccggcccagc
     8461 tcggcctgct tccttccgag ctctgcctgt ttctgcccca gctccttctg gcgctcgaac
     8521 aggtcgccgg cgcgccggag gaaggcggcg tcgcggatca cgtaggcctt gccgccctga
     8581 cggaaccaga gcacgtcgcc gtcccgctcc agcgcctcct ccatctcacg gtcggaggtg
     8641 gagccgttca tcgtcacgct gtcccccagc cgcaggaagt agtcgtcgcc gccgtcgccg
     8701 ttgcggtaga cgtggcggtg tcccttgccg tcggcggggg ccggcggagc gggtggggcg
     8761 ggcggcgccg gaggggccgg aggcgcgggg ggcggacccg tgcggttgcc tcgctgggcc
     8821 gccggggccg ccttgggagc catcgcgggg cgggccgcca cggcggggac cgctttcggc
     8881 gccgccttcg gagccaccgc cgggcgggca gccacggccg gagccgcctt cggggccggg
     8941 gcggctttcg tcgccgccac gggcttcgcg gcgcgcgggc cctccgcctc ggccgccact
     9001 atgcggtacg gcaccacgcc ggccagcagg actacgggga cgatccacca ggcgccggag
     9061 cggcgcgatc tctcggagct gttctgcagc atctgtaatc tccttttcag gttgaggaac
     9121 gagggggagg cgcccgcggc ggccagtccg gcctggcgcg ggctgacccc gaggcgcacg
     9181 agcagacgcc catagtcctg gggcgccgct ccgagcaggc gcagcacctc ggcatcgcag
     9241 gccgcctcgc gggccagggc gtactcccgg gcggcgaggg ccgccagggg gtggaagaag
     9301 aacagccgct ccgccagcgc cggcacccag ccgagccaga ggtcgccccg gcgcacgtgc
     9361 agggcttcgt ggcagagggt catggtgacc tcccccgggg agaggcgctc catggcgtcg
     9421 cggggcagca gcaccagcgg ccggagcagc cccagcgcct ggggggtcgc gatggccgtc
     9481 gaactgcgga gatccggcga gcggcgcagc cccagcagcc gcaccatctc cgcgaaggcc
     9541 tgccgcatcc aggggaccgc caccggctcc gccccccgga cgacgcgccg cgtctcgcgg
     9601 agctgcgcca gggcgagggc gagatggagc ccgagccccg tcagccagac acccagggcg
     9661 gcccatcccc aggggaagcc ggcggccggg gagggggccg cgggagccgc cggcgcgacc
     9721 ggcgcgctca ccgtctccca caccggcact gaagaagaaa tcaccggcgc ggcgggcgcg
     9781 gccgtcatcg tctccctagc gggcaggagc ggcagggcca cgggttcggt ccagacgagg
     9841 ccgaccagga gctggaggca ggccagccac cagagggcgc agcgtagcga ggcgggcagc
     9901 cgcggcagca gccggcagac ggcccagacc aggccgatga ggaccgcccc ctggagggag
     9961 gcgcgggcga ggagatccag agcctgctca gcgaaggcgt ccatctcagc tctccttctt
    10021 cctggacttg aggcggttga ccagatcctc cagctccgcc agctcctcgg ccgagacgtc
    10081 cccggtctcg gccaggtagt tgacgaacgg cgacaccgac cccgccaggg tcttctccac
    10141 gaacgaccgt accgcgccgc gcatcagctc gccgggcgac gcggtggacg cgtagcgata
    10201 gacgccgtcc accggctcgc gctccagatg ccccttctgg cgcaggcgct ccatcatggt
    10261 gaggacggtg gaccgcgcca ggccccgcgc cgtcccgaac ccctccaccg cctcccccac
    10321 cgacaccggc ccctgctcgg ccacgtaccg cagcagcgcc agctcctgat ctccgatgga
    10381 cttgctcgtc atgtctcccc tcgttgacta catctgtcgt catggtactc atgactacag
    10441 gtgtcgtcaa gaccctgata cgtggggagg gagagaaagg ttgcggggga agccgcccga
    10501 ctcgggctta gctcggccca gccaagccca ggtcgtcttc catgagcttg aatcgtctca
    10561 gatcagctcg gctcggggcg gataagctta cccgcgccag ataacgctat ctggaacgaa
    10621 acaagcttat cgagaagggg atagcgctag cggacccgac tcgagcttac ccaggaagag
    10681 ataacgctag ccagcccggg cgaagcttat ccggcgcgac ctgagctcag atgaagccgg
    10741 atagtcttgg cgggggcaag ctggcgttag acggtccaac tcgagcgtac ccagccgacc
    10801 tgagcttagc gggagcgatc taagctcagc ggagccgacc tgagcctgtg tgggggcaac
    10861 ttcgcgcctg gcctcttcat cggcggcttc tccagatctc agggtcgcgg gcgcggtgca
    10921 ggatggcgag gacccggatg gtttcgccgt cgatcagata gaagatccca taagggaact
    10981 gccctgtcgt ggccctacgg atacgggggc cgactcgggg aaaaagctgg gggtgtgctt
    11041 ggagcgtcgc gaggactgcc ttgacttcgg cgcggaagga ctcgcccaaa cctggtcgct
    11101 gctcttcata ccagcgatag gcctcgacca ggtcgagctt cgccgcccgg cggagcacca
    11161 gcctcatcgg tcaggccaga gctcggcttc tacttcttcc caggtctgct cgtcgtgggg
    11221 attccgctcc agatcggcga gtcgttcatc caagatccgc ctctgccaat ccgggatgga
    11281 gacgaaccgg gcctcgtcct tgggctccac gctttcccac aggacctggg cgatctcgcg
    11341 gcgctcctcg gcgggaaggt cgagaagctt ctggaggtcg gctcgtgtca tggagctagt
    11401 atagcttggc gtcctacacc agctcgaaca gcgggtcgcc gccttcgacg gcttggccgg
    11461 gggtgacgaa gacgtggccg atggtgcctt cgtgctcggc gcggatctcg ttctccatct
    11521 tcatcgcttc gagcacgacg acgccctggc cggcctggac ctcctggccc tcctcgacca
    11581 ggacggcgac cacccggccc ggcatgtagg cggtcacctt ctgctggcgc cgcccaccct
    11641 tgccccccgc cgcctgggcg gccaggtggg tgagcgggtc ggcgaccgtg accggactgg
    11701 taccctgggg ggtgctcacc cagtagacgc cgtcggcctg gggccgcacc gccacctcgt
    11761 ggtgggcgcc gtcgaggcgc aggctgtgga cgctcttccc cacggtggcc gcctccaccc
    11821 gccaggtgcg gtcgcccagg gtgacctcgt agacgtcgcc gtcgcggcgg acgcggacgc
    11881 gctcctcgcg ctctccatcg cggacgatca gctccatgcg ccctcccgca gcgcctcgcg
    11941 gcgggcggcg agcccccagc gcgagcgccg gctctggccg ccggaggccg ccggatccgc
    12001 caggctccgg ccgacgcgct cgaagtgagc gacggcggcg gcgatgagcg gcaggtccgc
    12061 cggctcgtcc ccgggcggcg gctgcagctc gccggcggcc agcttgcggt ccagcatgcc
    12121 gatgtccatg ttgccgctga cgaaatcggg gtcggccaac agcgcccggt agagggggac
    12181 ggtggtgcgg atgccttcga cccgcagctc ggcgagggcg cggccgaggc gcgccagcgc
    12241 cagcttccgg tccgccgccc agacgatcag cttggccagc atcgggtcgt agtagatgga
    12301 gacctccgac ccctcgtaga cgccggcgtc cacccgcacg ccggggccgt cgggccagcg
    12361 cagcagctcg atgcggccgg gcgacggcgc gaagcgctgg taggggtcct cggcgtagag
    12421 gcggacctcg atggcatggc cgcgcggccc gtgcgactgg agggcgtcga actccggccc
    12481 caacggctcc ccctcggcga cgcggatctg ggtgaccacc aggtcgatgc cggtcaccag
    12541 ctcggtcacc ggatgctcca cctgcaggcg ggtgttcatc tccaggaagt agaactcgcc
    12601 gtccgccgac agcaggaact cgcaggtgcc ggcgttgctg tagcccaccg cctgggcggc
    12661 cctgaccgcc gccgcgccca tgcgggcccg cagctccggc gtcaccaccg gcgacggcgc
    12721 ctcctccacc accttctggt gccggcgctg gagggagcac tcgcgctcgc cgagcgacac
    12781 caccttgccg tggaggtcgc ccatcacctg gatctcgacg tggcgcgggt tgacgatgta
    12841 cttctcgaga tagaccgagt cgtcgccgaa gctcgcccct gcctccgagc gggcggcgcg
    12901 gtaggcggcg ggcagctcgg ccgccgaggg gatcatgcgc atccccttgc cgccgccgcc
    12961 ggccgcggcc ttgagcatga ccggatagcc gatgtgctcc gccgccgccg ccgcggactc
    13021 caggtcggga agcgggtcct tgcccccggg gaccaccggc acgccggccg ccatcatcag
    13081 ccgccggctc tccaccttgg agcccatggc ggcgatcgcc tcgggcgatg ggccgatgaa
    13141 cgtcaccccg gcgtcgcggc agagccggga gaacgcctcg cgctcggaga ggaagccgta
    13201 gccggggtgg atggcgtcgg cgccgatccg caccgccagc tcgacgatcg cctcggcccg
    13261 caggtagctc tcccgcgacg gcgccgggcc gatcgggtag gcctcgtcgg cgagcagcac
    13321 gggcagcccc aggcggtcgg gctcggagta gatgacggcg ctacggatcc cgagctcgcg
    13381 gcaggcgcgc aggatccgca ccgcgatctc cccacggttg gcgatcagga ccttccggat
    13441 gcgcttcttc ggcacgtctg gcgatgtcgc gggcataggg ccgcctatct tatagcagcg
    13501 ggtctcttcc ggcagggcgc cggccccacc agaagaggac gggcaggagc agcagcccca
    13561 gcgcggtccc gccgagggag agctcgaagg ggcggcggtc gatccacaac cgcacccggt
    13621 gacggcccgc cggcacccgg atcccggcgc gctggaggtt ggcgttgagc acctcggccg
    13681 ggcggccgtc caccgtcgcc ttctggagca gggcggagcg ctggaccacc agcaccgagc
    13741 cgccgggccc cacgtccgcc tccacctcca gctcctccgg ctcgtccttc acgatccgga
    13801 cggtgccgcc gcccagcgcc tcgggcttgc cgttccccgg cagcactacg tccctgcgag
    13861 cgttgaagct gctcacccct cccatggcac cgctcagggt cctgtaggcg gcgttcatgt
    13921 gcggggcgaa gacggcgcgg tgggcgagga agacctcggg cgcccggtcc aggacctcat
    13981 agatgtagag gtggaagccg aagctcggga tcgccgcgag ctcccgcacc cccggcgtgt
    14041 ccggagtcag agggtgattc atgaggaggc gccccacccc ccaggcggcc agcatccgga
    14101 tgcgctgcgt gtcgttggac ccccggaccg ccccctgggc catgcgggcg aggtaggtgt
    14161 ccagcccctc gggggagacg ttgaggtcgt agcgccgccc ccactgcggc ccggtgaacg
    14221 agtacagctc gtagaaggcc cggcgctcca gccaattgcc cttgcgctca gggaacttgc
    14281 cgtcgcgcag ggtgtgcttg ccgaacaggc tgttcaggtc cgggttgacc acccgcacgt
    14341 ccgccggcac gtagtccagc gccggcggcg ggatcaggta gggctggatc gggtcggtcg
    14401 ggtaggccgg ccggagcagc aggacctggg acaccgcatg gacggcgagg atgaccgcgc
    14461 cgcccagcgc cggccggcgg cgcagcaggc gcgccccggc cgccagcccg gcgagcaccg
    14521 cgaacgagaa caggcagagc ccggcccagc gcagccgctc gttgcggacg aacggctgct
    14581 ccgtctggaa cgccttgggg atgatccgcg acgccagctc ctccgccgtg ccgggctgga
    14641 aggtgaggaa ggcccagaag ccgccgagca ccaggaacag cccgaggagc gccaggccga
    14701 accgccgccg ggcgcgccgc gtgagcgggt cgtccgccgg cccgagcagc cgttggaaac
    14761 acaggccgca cagcagcgcc gcccccaccg ccagggggag ccagagcttg accgggtagc
    14821 gcagcgcgcc gttgcgcaag gtgaagaccc actccgccgc cgggttgaac cgtccgaggg
    14881 agaagaagag gccgaagacg accgcgcccc aggcccacca ggcggcgcgg cggcgcggcc
    14941 ggcccgacgc cagcaccagc gccagggtca ggaggcccgg gtagagggtg acgtagtagg
    15001 gggggacgcc ggtgaagaac ttcttgcccc agaagctcgc gaggctcagc atgtccagcc
    15061 ggccgaagat catggggagg aaccactcgg cgacctggta gggatcccaa ctcgccagcg
    15121 tcgccacctg cggttcgtac cccttctggc cccggaacga caacggcagg atgcgcagga
    15181 actcgacgag ctggggcagc gccagcagcg tccccgccgt gaagcacccc gccaggaggg
    15241 ccaagcgccg ccacccc
//
LOCUS       DASPJI010000006         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_458333, whole genome shotgun sequence.
ACCESSION   DASPJI010000006 DASPJI010000000
VERSION     DASPJI010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458333"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2923
                     /locus_tag="VEL74_00165"
     CDS             <1..2923
                     /locus_tag="VEL74_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HYG60966.1"
                     /translation="GAGAVYAGNAAVGQGSITSQPAVGGTGDVVWNPGTLAAGATSIL
                     TYQVDISPTSAGQRILATSTPASGNGTRARFVDETGNTTQARATYLAGPICELGVTEG
                     LLTEAVVASFRARQEGNAVVLEWRTASEAGTAGFYVYGRDILEGADTEPFQLHENLLP
                     GLLHAPQGGTYRFVDESASPRVPREYVLIEVEADGRLRPHGPFRPAVEWGNEWDKSAL
                     EPGLAYDRVAHPAKAAPVSKRAPARGTATAGSPRALRVAVRESGLYRISSADMAGLFE
                     ISRQDMEKKVAEGKLSLTRGGQPVAWYPFFDGSAAAGPRAAKGAQGIYFYGQALDSLY
                     SLDNVYRLDLGGSGLLMQVAAVSGPGAAPPAASFAEVGRSERDLLPATAVAPDAESDY
                     WFWDFLQAGDATYGRKTYRLDAPGLTSGTTASLTVRLFGATTTGVTGEHRVSVSVNGT
                     PVGETSWQGIAPRSATFAVDPALLLETGNQVEATALLDAGVPYSIFYIDSFELGYPRA
                     HRAAGDVLAFTGSGAAASVSGLSAAPVLLDVSVPARPRWLTGATVEPDAASGAGFRAG
                     FAAAAAGARYLATGPGGVKTPDLRAWRDEGLAASTNGADYLVITTAGLGPAAQRLANY
                     RAAQGLESLVVDVEAVYDEFNAGLPSPHALRAFLAHARRSWRKAPRYVVLAGAGSIDY
                     RDLLGYGGGLVPPLLVRSSGGLFVSDNAIADVAGGAGGDGLPEMAIGRLPVRTPAELN
                     AYIDKLGAAEAAGVAGPEWRSRVLMLSDAPDRGADFAADNEKVAGRLPDAYSVDRIDL
                     GAEPLEAARGRLFSGLARGAALVNYLGHGGLDRLTGAGLLASADIPAMAAGPRLPVLT
                     AMTCTVNRFGVPGVSSLGEVLVRQPGRGAAAVWSPSGLSIHGEAVLLAERFYQTATDP
                     SSAATATRLGDLVLRSLGDLKTLGGSRSMLDVYNLLGDPALLVQQPVPAPPTGAGGGG
                     E"
     gene            2935..3102
                     /locus_tag="VEL74_00170"
     CDS             2935..3102
                     /locus_tag="VEL74_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60967.1"
                     /translation="MGPTTFGQEQNGTRRPYEPPRILYREPLEAMAAVCAPTPPAKGN
                     PGFCPQGPISS"
     gene            complement(3109..>3554)
                     /locus_tag="VEL74_00175"
     CDS             complement(3109..>3554)
                     /locus_tag="VEL74_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HYG60968.1"
                     /translation="KARAERGGGGSVARGGDDGTMPAPVHKHCGAGSAGIAVDPFGNV
                     YPCVQWRRPVGNLHERPIGEIWTGSPALREVRELTAEAKTRVDAYGPAGAFLNFCPGA
                     AATRTGDPLRIYPDAVRRMELMEEVLGQRTGASEDNGPLLPILRD"
BASE COUNT          449 a         1367 c         1276 g          462 t
ORIGIN      
        1 cggcgccggc gcggtctacg ccggcaacgc cgcggtcggc caggggagca tcacctccca
       61 gccggcggtg ggcggcaccg gcgacgtggt ctggaacccc ggcaccctgg cggccggcgc
      121 cacgtccatc ctcacctatc aggtggacat ctcccccacc tccgccggcc agcgcatcct
      181 ggcgacctcc actcccgcgt cgggcaacgg cacgcgggcg cggttcgtcg acgagacggg
      241 caacaccacg caggcccggg ccacctacct ggccgggccg atctgcgagc tgggggtgac
      301 cgaagggctg ctgaccgagg ccgtggtggc ctcgttccgg gcacgccagg agggcaacgc
      361 ggtggtcctg gagtggcgga ccgcgtcgga ggcggggacc gccggcttct acgtctatgg
      421 ccgcgacatc ctggaagggg ctgacaccga gcccttccag ctgcacgaga acctgcttcc
      481 cggactgctc cacgcgccgc aggggggcac ctatcgcttc gtcgacgaga gcgcctcgcc
      541 gcgggtgccg cgcgagtacg tgctgatcga ggtcgaggcg gacggccgcc tgcggcccca
      601 cggccccttc cggccagccg tcgagtgggg caacgagtgg gacaagtcgg cgctggagcc
      661 cggtctggcc tacgaccgcg tggctcatcc ggccaaagcc gctccggtct cgaagcgggc
      721 gcccgcccgc ggcaccgcga cggccggctc gcccagggcg ctgcgggtcg cggtgcgcga
      781 gagcggcctc taccggatct ccagcgccga catggccggc ctcttcgaga tctcccggca
      841 ggacatggag aagaaggtgg cggagggcaa gctctccctc acccgcggcg gccagccggt
      901 cgcctggtac cccttcttcg atgggtccgc ggccgcgggg cctcgcgccg ccaagggagc
      961 gcaggggatc tacttctacg gccaggccct cgacagcctc tactcgctgg acaacgtcta
     1021 ccggctcgac ctcggcggca gcggcctcct catgcaagtg gcggcggtgt ccggcccggg
     1081 cgccgcgccg ccggcggcga gcttcgccga ggtcggccgc agcgagcggg acctgctgcc
     1141 ggcgaccgcc gtggcccccg acgccgagtc cgactactgg ttctgggact tcctccaggc
     1201 gggcgacgcc acctacggcc gcaagaccta cagactggac gcgccggggc tgacgagcgg
     1261 cacgacagcc tctctcaccg tgcggctgtt cggcgccacc accaccggcg tgaccggcga
     1321 gcaccgggtg agcgtgtcgg tcaacggcac cccggtgggc gagacctcct ggcaggggat
     1381 cgcgccgcgg tcggccacct tcgccgtgga ccccgcgctg ctgctcgaga ccggcaacca
     1441 ggtggaggcg accgccctgc tggacgccgg ggtcccctac agcatcttct atatcgactc
     1501 cttcgagctc ggctaccccc gggcccaccg cgccgcgggc gacgtcctgg ccttcaccgg
     1561 cagcggcgcg gcggcctccg tgtccggcct gtcggcggcg ccggtgctcc tcgacgtcag
     1621 cgtcccggcc cggccgcgct ggctcaccgg cgcgacggtc gagcccgacg cggcctccgg
     1681 ggccggcttc cgggccggct tcgcagccgc cgccgcgggc gcccgctacc tggcgacggg
     1741 cccgggcggc gtcaagacgc cggacctgcg ggcctggcgc gacgaggggc tcgccgcctc
     1801 caccaacggc gccgactacc tggtgatcac caccgccggc ctggggccgg cggcgcagcg
     1861 gctggccaac tatcgcgcgg cccaggggct ggagagcctg gtggtggacg tcgaggcggt
     1921 ctacgacgaa ttcaacgccg gcctgccgag cccgcacgcg ctgcgcgcct tcctcgccca
     1981 cgcgcggcgg agctggcgca aggccccccg ctacgtggtg ctggcgggcg ccggcagcat
     2041 cgactatcgc gacctgctgg gctacggcgg cgggctggtg ccgccgctcc tggtgcgcag
     2101 ctcgggcggc ctgttcgtct cggacaacgc catcgccgat gtcgcgggcg gtgccggcgg
     2161 cgatggcctg ccggagatgg cgatcgggcg cctgccggta cgcaccccgg cggagctcaa
     2221 cgcctacatc gacaagctcg gcgccgccga ggccgccggc gtcgccggcc cggagtggag
     2281 aagccgtgtc ctgatgctct cggacgcccc cgaccgcggc gccgacttcg ccgccgacaa
     2341 cgagaaggtg gcggggcgcc tcccggatgc ctactcggtg gaccggatcg atctcggggc
     2401 cgagccgctc gaggcggcgc gcggccgtct gttctccggc ctcgcccgag gggcggcgct
     2461 ggtcaattac ctggggcacg gcggcctcga ccggctcacc ggcgccggcc tcctggcgag
     2521 cgccgacatc ccggccatgg ccgcgggccc caggctgccg gtcctcaccg ccatgacctg
     2581 cacggtgaac cgcttcgggg tgccgggcgt ctcctccctg ggcgaggtgc tggtgcggca
     2641 gcccgggcgg ggagcggcgg cggtctggag cccctcgggc ctctccatcc acggcgaggc
     2701 ggtgctgctg gccgagcgct tctaccagac ggccaccgac ccctcatcag cggcaacggc
     2761 gacgcggctg ggcgacctcg tcctgcgctc cctgggggac ctcaagacgc tgggtggcag
     2821 ccgctcgatg ctcgatgtct acaatctgct gggggatccg gcacttcttg tgcagcagcc
     2881 ggtaccggcg cctcccaccg gcgcaggcgg cggcggggag tgatgaaagg agccatggga
     2941 cccacgacct ttgggcagga gcagaacggc acccggcggc cctatgagcc gccgcgcatc
     3001 ctctaccgtg agcccctgga ggccatggcg gcggtctgcg cgcccacccc tcccgccaag
     3061 gggaaccccg gcttctgccc gcaggggccg atctcgtcgt aggaaggctc agtcccgcag
     3121 gatggggaga agcggcccgt tatcctccga cgcccccgtc cgctgcccca gcacctcttc
     3181 catcagctcc atccgccgca ccgcgtccgg gtagatccgc aacggatcgc cggtgcgggt
     3241 ggccgccgcg ccgggacaga aattgaggaa ggcgccggcg ggtccgtagg cgtccacccg
     3301 cgtcttggcc tcggccgtga gctcccgcac ctcccgcagg gccggcgagc cggtccagat
     3361 ctccccgatc ggccgctcgt gcaggttccc gaccggccgc cgccactgga cgcaggggta
     3421 gacattgccg aaagggtcga cggcaatccc cgccgagccg gcgccgcagt gcttgtgaac
     3481 cggggccggc atggtgccgt cgtcgccccc ccgggccaca ctccccccgc cccccctctc
     3541 cgcccgcgcc ttca
//
LOCUS       DASPJI010000007         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_229352, whole genome shotgun sequence.
ACCESSION   DASPJI010000007 DASPJI010000000
VERSION     DASPJI010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229352"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..827
                     /locus_tag="VEL74_00180"
     CDS             168..827
                     /locus_tag="VEL74_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60969.1"
                     /translation="MKCRGFFGSRFGNQMARAGCVLAAALLWDGPAEAAPAIAFEPAA
                     VNVSGMTPQGRAFVFGVAWAADGRVKTSRHTTFLSDGDGNGEARLDLGTERPDTSVWL
                     AVDFEDGSWTVATTGGAVREARFPWNAVPYARDRLALHSEKAYIAIVRPLIGAWLLRV
                     NDGGESDGDGDNNGQIRAGLASFSAFGGSPPPPAKLERGDIVLVVDPERLLFSRHALA
                     R"
     gene            complement(850..1350)
                     /locus_tag="VEL74_00185"
     CDS             complement(850..1350)
                     /locus_tag="VEL74_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG60970.1"
                     /translation="MDSKIFGGVIKTWRLRADLSQKDLAEKSGLSESTVGAIERGERR
                     LSDENFVKICIGLGMTVDEIFDSGSHIHLDVLHQIERRLRGEKDSEAPRREAAGQPSL
                     EQISELLDSWTDQMKELLLSIFKYVRASGPEARFRIPPAASRDLPAGKSQRARVGRSR
                     QRGPRK"
     gene            1657..>2229
                     /locus_tag="VEL74_00190"
     CDS             1657..>2229
                     /locus_tag="VEL74_00190"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYG60971.1"
                     /translation="MKALIFEGIRQVRCATVPDPAPLGPGEVVVRVGAAAICGSDLHV
                     YRGAEAGLDAGTVLGHEMAGEVVAAGPEVRRFRPGDRVVSPFTTSCGRCFYCRTGLTA
                     RCVEGQLFGWVQEGRGLHGVQAEYARVPLADSTLVAVPGGVPLEAALFAGDVLATGWY
                     AAESAGVGPGAVAAVVGCGPVGLMAALAARE"
BASE COUNT          307 a          748 c          820 g          354 t
ORIGIN      
        1 ggggacacgg attctacctc gcccgtctct gcccgtggac gcggctgtcc tgaaaggggg
       61 gctccgtgtg cctcccgccg ccgcggccgg gcgctccacg gcaggctcga tttttgcaaa
      121 aatacggctc agccggacat ctgtttctcc aacgaggagg tgccaaattg aagtgccgag
      181 gattcttcgg aagccgcttc gggaatcaaa tggcgcgggc tggctgtgtc ctcgccgccg
      241 cgctgctgtg ggacgggccg gcggaagccg cgcccgccat cgccttcgag ccggccgcgg
      301 tgaatgtctc gggcatgacc ccccagggcc gggcgttcgt cttcggcgtc gcctgggctg
      361 cagacggccg ggtcaagacc tcgcggcaca ccaccttcct gagcgacggg gacggcaacg
      421 gcgaggcgcg tctcgatctc ggcacggaga ggcccgacac ctcggtctgg ctcgccgtcg
      481 atttcgagga cggctcgtgg acggtagcca ccaccggcgg tgcggtgcgc gaggcgcgct
      541 tcccttggaa cgccgtcccc tacgcccgcg accggttggc cctccactcg gagaaggcgt
      601 acatcgcgat cgtccgcccc ctgatcggcg cctggctcct gcgggtgaac gacggcggcg
      661 agagcgacgg cgacggcgac aacaacggtc agatccgggc cgggctggcc agcttcagcg
      721 ccttcggcgg ctccccgccg ccgccggcga agctcgagcg cggcgacatc gtcctggtgg
      781 tggacccgga acggctgctc ttcagccgcc acgccctggc tcggtgaccg cggctgcgcg
      841 ggtgacggat cacttccgcg ggccgcgctg gcgggagcgg cccacccgcg cgcgctgcga
      901 cttgcccgcg ggcaggtccc gcgaggccgc gggagggatc cggaaccgcg cctccggccc
      961 cgaggcccgg acgtatttga agatggagag caggagctcc ttcatctgat ccgtccagga
     1021 gtcgaggagc tcgctgatct gctccaggga cggctggccc gccgcctccc ggcggggcgc
     1081 ctcggaatcc ttctccccgc gcagccgccg ctcgatctgg tgcaggacgt cgagatggat
     1141 gtgggagccc gagtcgaaga tctcgtccac ggtcatcccc aggccgatgc agatcttcac
     1201 gaagttctcg tcgctcagcc gccgctcgcc acgctcgatg gccccgacgg tgctctcgct
     1261 gagccccgac ttctcggcaa gatccttctg gctgaggtcg gcccgtaacc tccaggtctt
     1321 gatgacccct ccgaagattt tggaatccat ggccgcggat cgtactgacc gctctgcgca
     1381 tggtcaaaag ccggaaaagg tgactgaaaa ttacgaatcg cgagcaaatc ccccgtgttc
     1441 cgtgaagttg tccgctctgt gcggcggaag agacgccttg acctcaccgg ggagcggagt
     1501 tggcccaccg ttgccacgaa agggcgacat gtttctgtga ggggtgcaaa ggtccagccg
     1561 aagaaagctg gtggggcggt gtccggcgag attagtgccg ggaatgggtg cggcagggcg
     1621 tctggcgtaa gcttctttcg ggaggaaggc gaatccgtga aagccctgat cttcgaagga
     1681 attcggcagg tccgctgcgc caccgtgccg gatccggcgc cgctcggccc gggagaggtg
     1741 gtggtgcggg taggggcggc ggcgatctgc ggctcggacc tccacgtcta ccggggagcg
     1801 gaggccgggc tcgacgcggg gaccgtgctc ggccacgaga tggcggggga ggtggtggcg
     1861 gcggggcccg aggtccgccg cttccggccc ggcgaccggg tggtgagccc cttcaccacg
     1921 agctgcggcc gctgcttcta ttgccggacc gggctcaccg cgcgctgcgt ggaggggcag
     1981 ctcttcggct gggtccagga ggggcggggc ctccatggcg tccaggccga gtacgcccgc
     2041 gtgcccctgg cggactcgac gctggtggcc gtccccggcg gggtgccgct ggaggcggcg
     2101 ctcttcgcgg gcgatgtcct cgccaccggc tggtacgccg ccgagtcggc cggggtgggg
     2161 ccgggagccg tggccgcggt ggtcggctgc ggccccgtcg gcctgatggc cgcgctggcc
     2221 gcccgcgag
//
LOCUS       DASPJI010000008         3616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_430, whole genome shotgun sequence.
ACCESSION   DASPJI010000008 DASPJI010000000
VERSION     DASPJI010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1050
                     /locus_tag="VEL74_00195"
     CDS             91..1050
                     /locus_tag="VEL74_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HYG60972.1"
                     /translation="MSPAAALTPAAPFPRRLAAYLSERFPVLGHGILILAYYSSNQFL
                     ARTLTEPGRPMHYDWTTVLGAVTLFCFFFHLRVFDEHKDYAEDLVHYPQRVLQSGLIT
                     LRHLKILGGAAIALEVTLSAVRGPAALAGWALAFGFSLLMLKEFFARDWLKRHFLVYA
                     TSHMLLMPLLSLMVFSFATGRWPWEAPGWFWVYAWVGFFVTFNWEVSRKIRAPEDEIE
                     GVETYTRIFGTYGAAWIVLLIRVIDTALVALVGWHLGLAPWFYLALIALFAVCLVGFY
                     QYRAHTNRKTAKRMEVYAGMYIIAFDLILALAIAAKHGVELVG"
     gene            1047..1373
                     /locus_tag="VEL74_00200"
     CDS             1047..1373
                     /locus_tag="VEL74_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60973.1"
                     /translation="MISESDAGRRGLLILFFVILALPFTLLAVGWAARLLSTAVSQGP
                     DPGLECLYGSLIGLFILVPPGALGLGFDFLLAKTPGERQIIYVVSAALLGAAAVMIGT
                     LFVVYS"
     gene            1381..>3616
                     /locus_tag="VEL74_00205"
     CDS             1381..>3616
                     /locus_tag="VEL74_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HYG60974.1"
                     /translation="MTNLLPLVAADPQAAAALPTTLGGKAANLARLEAQGLPVPPWYA
                     VSTHAFERALATAALRERIAARLETAGSDDSLRRASEEIRGWIAALDLPSDLIREIEQ
                     AHTARIPGDSFVAVRSSAAGEDAAGESFAGLHDSFLFIRGRERVLDAVRRVWASGYNE
                     RALAYRRAKGIPLDSIAVGVVVQQMVDARASGVLFTANPNTGDVHEIVISSLWGAGEG
                     LVSAGFDADTFVIDKETREIAPEVVSKRERLLLDAEAGGGLVRTEVPADLQDRPSLSN
                     DHILELARVGLAVERQLRRPQDIEFAVDAAGRLYLLQARPVTTVEELGPAAGNPLIWD
                     NSNIIESYSGVTSPMTFSFIRRAYSIVYHCFAEVMGIDERAVRANREVFENMLGLFRG
                     QVYYNILNWYRLVRLFPGFEYNRAFMESMMGVRESARLAENEEKKERGAWQRYTVELP
                     ALVRLVGRSVWNFVRIRRIVGDFQIYFRERYERWSALDFRAMAPHELAALYRKMEDEL
                     LWQWKAPIINDFFVMVFYGTLKKLCVSWCGDESGSLQNDLLAGEGGIESTEPTRMLLG
                     LAARARQEPELQKVLLERTPEELAREIPTDPRFPDFAAQIARYLDLYGFRCVNELKLE
                     ELSLRDRPAFLYQVLRNYLGGDPAALDVSAMEEREQRIRKEAEARAFAAIRSKGGLFP
                     RASIFRRVLGNARLGVKNRENLRFARTRIYGLLRELLRAMGGHFAAAGLLDREEDVFY
                     LTLDE"
BASE COUNT          522 a         1240 c         1178 g          676 t
ORIGIN      
        1 ccggacgtcc gaagcgctgc cggtcgatcc gcgccacaac agcaagatcg actacccgcg
       61 tctgcggagc gatctgatgg cgaggcgccg gtgagcccgg ccgccgccct caccccggcc
      121 gcgccgttcc cacggcggtt ggccgcctat ctttccgagc gctttccggt gctgggccac
      181 ggcatcctga tcctcgccta ctactcttcg aatcagtttc tggcgcggac gctcaccgag
      241 ccgggccggc ccatgcacta cgactggacg acggtcctgg gcgcggtgac cctcttctgc
      301 ttcttcttcc atctccgggt cttcgacgag cacaaggact acgcggagga tctcgtccac
      361 tatccgcaac gggtgctcca gagcggattg atcaccttgc ggcatctgaa gatcctgggc
      421 ggcgcggcca ttgccctgga agtgaccctg tcggcggtcc ggggacccgc ggcgctggcg
      481 ggctgggcgc tggccttcgg cttctcgctc ctgatgttga aagagttttt cgcccgcgac
      541 tggctgaagc gtcactttct cgtctacgcc acgagccaca tgctgctgat gccgctcctg
      601 tcgctgatgg tcttcagctt cgcgaccggc cgctggccct gggaggcgcc aggctggttc
      661 tgggtctacg cctgggtggg cttcttcgtc accttcaact gggaggtgtc gcgcaagatc
      721 cgcgcgccgg aggacgagat cgagggcgtg gagacctaca cccgcatctt cggcacctac
      781 ggcgcggcct ggatcgtcct cctgatccgc gtgatcgaca ccgccctggt ggccttggtc
      841 ggctggcacc tgggcctcgc cccctggttt tacctcgccc tgatcgccct cttcgccgtc
      901 tgcctggtgg gtttctatca atatcgcgcc cataccaacc gcaagacggc gaagcggatg
      961 gaggtctacg cggggatgta catcatcgcc ttcgatctga tcctggcgtt ggcgatcgcg
     1021 gcgaagcacg gagtggagct ggtcggatga tctccgagtc ggatgccggg cggcgagggc
     1081 tgctcatcct tttcttcgtc atcctggcgt tgccctttac gcttctcgca gtcggctggg
     1141 ccgccaggtt gctctccacc gctgtttccc agggacctga tccaggcctc gagtgcctct
     1201 atggcagtct cataggtttg tttatcttgg taccgccagg agctctcggg cttggcttcg
     1261 actttttgct ggccaagacg ccgggtgaga ggcagatcat ctacgtcgtc tcggccgcgc
     1321 ttctaggagc cgcggcagtg atgattggca cactcttcgt ggtctattcc tgagaacggt
     1381 atgaccaacc tcctccctct cgtcgccgcc gacccccaag ccgccgctgc cctccccacc
     1441 accctgggag gcaaggcggc caacctggcg cgcctcgaag cccaggggct gccggttccc
     1501 ccctggtacg cggtttccac ccacgccttc gagcgtgccc tggccacggc ggccctgcgc
     1561 gagcgcatcg ccgcgcggct ggagacggcg ggctcggacg attccctccg ccgcgcttcg
     1621 gaggagatcc gcgggtggat cgctgccctc gatctcccct cggacctcat ccgggagatc
     1681 gagcaggccc acacggcccg catcccgggt gattccttcg tcgccgtccg ctcgtcggcg
     1741 gcgggggagg acgcggcggg ggagtcgttc gcggggctcc atgacagctt tctcttcatc
     1801 cgcggccggg agcgggtgct ggacgccgtc cgccgggtgt gggcgtccgg ctacaacgag
     1861 cgcgctctgg cctatcgccg ggccaagggc atccccctcg attcgatcgc cgtcggcgtg
     1921 gtcgtgcagc agatggtgga cgcgcgggcg agcggtgttc tgttcaccgc caatcccaat
     1981 acgggggatg tccacgaaat cgtgatcagc tcgctctggg gcgccggcga ggggctggtg
     2041 agcgccgggt tcgacgcgga cacgttcgtg atcgataagg agacgcggga gatcgccccc
     2101 gaggtggtgt ccaagcgcga gcggcttctc ctcgatgccg aggcgggcgg cggcctcgtg
     2161 cggaccgagg tgccggcgga cctccaggac cggccgagcc tgtcgaacga tcacattctc
     2221 gagctcgccc gcgtcggcct cgccgtggag cgccagctcc gccgtcccca ggacatcgag
     2281 ttcgcggtgg acgcggccgg ccggctctac ctcctccagg cgcggccggt gacgacggtg
     2341 gaggagctgg gcccggcggc gggcaatccg ctgatctggg acaactccaa catcatcgag
     2401 agctactcgg gcgtgacctc gccgatgacg ttcagcttca tccggcgcgc ctactccatc
     2461 gtctatcact gcttcgccga ggtcatgggc atcgacgagc gggcggtgcg cgccaaccgc
     2521 gaggtcttcg agaacatgct cggcctcttc cgcgggcagg tctactacaa cattctcaac
     2581 tggtaccgcc tggtccggct cttccccggc ttcgagtaca accgcgcctt catggaatcg
     2641 atgatgggcg tgcgggagtc ggcgcgcctc gcggaaaatg aagagaagaa ggagaggggc
     2701 gcctggcaac gctacacggt ggagctgccc gcgctggtcc gcctcgtcgg ccgctcggtc
     2761 tggaacttcg tccgcatccg ccgcatcgtc ggcgacttcc agatctattt ccgggagcgc
     2821 tacgagcgct ggtccgccct ggatttccgg gccatggccc cgcacgagct ggcggctctc
     2881 taccggaaaa tggaggacga gctcctctgg caatggaaag cgccgatcat caacgatttt
     2941 ttcgtgatgg tcttctacgg gacgctcaaa aagctctgcg tctcctggtg cggcgacgag
     3001 tccggctccc tgcagaacga cctcctcgcc ggcgaagggg gtatcgagag caccgagccc
     3061 acccgcatgc tcctcggtct cgcggcccgg gcgcggcagg agccggagct tcaaaaagtg
     3121 ctcctggagc gcacccccga ggagctggcg cgggagatcc ccaccgatcc gcggttcccg
     3181 gacttcgccg cccagatcgc ccgctatctg gacctctacg gcttccgttg cgtcaacgag
     3241 ctcaagctcg aggaactgtc gctgcgggac cgccccgcct tcctctacca ggtgctccgc
     3301 aactatctgg gaggagatcc cgcggcgctg gacgtctcgg cgatggagga gcgggagcag
     3361 cgtatccgca aggaggccga agcccgcgcg ttcgctgcca tccggagcaa agggggcctc
     3421 ttcccgcggg cttccatctt caggcgggtg ctgggcaatg cccgtctcgg agtgaagaac
     3481 cgcgagaatc tccgcttcgc ccgcacccgc atctacggcc tgctgcgcga gctgctccgc
     3541 gccatgggcg gccacttcgc cgccgccggc ctgctggacc gggaggagga cgtcttctat
     3601 ctgactctcg acgagg
//
LOCUS       DASPJI010000009         7702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_572481, whole genome shotgun sequence.
ACCESSION   DASPJI010000009 DASPJI010000000
VERSION     DASPJI010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7702)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7702
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572481"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VEL74_00210"
     CDS             complement(<1..582)
                     /locus_tag="VEL74_00210"
                     /inference="COORDINATES: protein motif:HMM:NF028457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HYG60975.1"
                     /translation="MRRAAIVCLAVFLCLPVSGVAGEGEKTFQDLTQGAVLHEGVFDV
                     YRKGDHLYVAIPPDRLGRDMVLVPRLERGIGAAGLFGGLMFDRQAASIVAFERHGDRV
                     FLVKRAHRFTAPAGSAEAAALAISIGESVLQSATVAATRPKDSAVVIDVHDWMVSDLS
                     NIDKLLRTALKPGRATLDRSRSYVSEVKSFPRNL"
     gene            798..1271
                     /locus_tag="VEL74_00215"
     CDS             798..1271
                     /locus_tag="VEL74_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HYG60976.1"
                     /translation="MDPDRIDYPRIVQDALRDAVRRVLEQAAEHGVPGEHQLYIAFRT
                     QHPQAKVPPFLRDQYPEEVTIVLQHQYWDLEVTAEAFSVTLNFNAVRQRLTVPFAAMT
                     AFFDPAAEFALRFEPNPGEAAATEAPAAAEPAPEAPRPAPAKPGEVIRFDPSRRK"
     gene            1491..2636
                     /locus_tag="VEL74_00220"
     CDS             1491..2636
                     /locus_tag="VEL74_00220"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HYG60977.1"
                     /translation="MRFTQKLFPVLLCGTVLLAAGCGRDAEQEEARVVGEEASQSRPA
                     DEDKGYVIEEPGLTSEMATGTEIAAEGEPEALEAQEESQEAAELRRETVVDIDQGTRP
                     SPPAAREQSRQTQAAELEARERRLEQRQAELEARERELSRRERPQRPAARPERPAPAE
                     ETEVPVEIEETAEVGETSRPVEVEPEVEADPEERAVRELPRTAAASATVPTGTLMEVE
                     VLERLASDTTRAGETFRARIVSDLRSDDGRVAVPAGSEIAGVVDEVVPLRKVGGRAKM
                     ALRFTDLVLPSGTTVPIRASLVQEGRSETKKDAATIGGATAGGAILGRILSKRGDRSR
                     ATVIGAIIGAAAGTAIASRSPGEEVVIAEGTVVDLSLDQDVEIRPRR"
     gene            2793..4778
                     /locus_tag="VEL74_00225"
     CDS             2793..4778
                     /locus_tag="VEL74_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG60978.1"
                     /translation="MTDLSAPSSRRGARPGLFGGQVSSKQRGPARDPSEAAPSETDPQ
                     KQDKKKPNFRAFLGDARELIRARRGRLILGLGLMLISRLASLVLPGTSKYLLDEVIGK
                     QQRQLLVPLILVAGTATLLQAVTSFALSQVLGKAAQRSITEMRRRVQRHVGRLSVRYF
                     EQTKSGALLSRVMNDAEGLRNLVGTGLVEIVGGLVTAVLAVGILFFLSAKLTLIAIVV
                     LSLFGFVLKFAFKNLRPLFRERSRINADLAGRLTESFNGVRVVKAYGAERREALVFTK
                     GAHRLFRNVARTITGFSAIGAASSLLLGAVSVSIMLVGTQEILAERMTVGDFVAFTLY
                     MGMLVGPVVQIVNIGSQITEAFAGLERIREIRAETPEDEDEASRRPMGRIDGRVELRD
                     VWFEYQADAPVLKGISFTASPGTSTALVGPSGSGKSTLIGLVAAFHRPTAGAILVDGQ
                     DLATVKLRDYRSQLGVVFQDNFLFDGTVFENIAFARPHASEEEVVRAARIARCDDFVQ
                     KLPQGYETVVGERGVKLSGGERQRVAIARAILADPRILILDEATSSLDSESEAAIQEG
                     LAELMKGRTTFVIAHRLSTVRRADTIMVLEGGEIVERGRHEELLALGGRYHALYTRQY
                     NLESNLFRNPGEKVEEEEEAKPAVVADASVGRLPLLG"
     repeat_region   5024..5746
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5097..5133
                     /rpt_unit_seq="gtcgcaatcccaccaaaccgctctggtcaattggaag"
     gene            complement(6296..6652)
                     /locus_tag="VEL74_00230"
     CDS             complement(6296..6652)
                     /locus_tag="VEL74_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60979.1"
                     /translation="MNTRPDGTPTPPSDPASRPDIDLTEETEETRRKLYDAAVATHFT
                     GTPEERDKAKADPDAWQPPAVTPDQAKLAVHHLFGRWFVVWRPMEEGNLPPDRRWTVL
                     RVVQDPDRGTLDYHEV"
     gene            complement(6995..>7702)
                     /locus_tag="VEL74_00235"
     CDS             complement(6995..>7702)
                     /locus_tag="VEL74_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60980.1"
                     /translation="RPPRASSSSCRPRSTISPPSSTMIVSARPPQPPAAERLEQARLL
                     VAEGEDDRARQILATFTAADEAALPPSACRLLDGLRETLLLAARDRLPDDLAAGLDGN
                     LARLRSAVDTGIAQPELVAALSPEAQADFGRARRAMELYIQAEGAARNGEHPAVLEHL
                     AALLALSPELTDPEELRKKSADALEAEAVAEQRSRGPRPAENVAEPRCFVADPHNSAT
                     NAHRSATNERSSATIEG"
BASE COUNT         1233 a         2663 c         2648 g         1158 t
ORIGIN      
        1 caggttgcgc gggaacgact tgacctccga gacgtagctc cggctccggt cgagggtcgc
       61 ccggccgggc ttcaaggcgg tgcgcagcag cttgtcgatg ttcgacaggt ccgagaccat
      121 ccagtcgtgg acgtcgatca ccacggcgct gtctttcggg cgggtggcgg ccaccgtcgc
      181 cgactggagc accgactcgc cgatcgagat ggcgagggcg gcggcctcgg cggagccggc
      241 cggcgcggtg aagcggtggg cgcgcttgac caggaagacc cggtcgccgt ggcgctcgaa
      301 ggcgacgatg ctcgccgcct ggcggtcgaa catcaggccg ccgaacaggc cggcggcgcc
      361 gatgccccgc tccagccgcg gcaccagcac catgtcgcgc cccagccggt cgggggggat
      421 cgccacatag aggtggtccc ccttccggta gacgtcgaac acaccctcgt gcaggacggc
      481 gccctgagtg aggtcctgga acgtcttctc gccctcaccg gccacgccag agaccggaag
      541 acagaggaag acagcgaggc aaacgatcgc ggcacgtctc atcaccaaag gcctccatga
      601 ggtctgccga gagtcccctc ctggacgtcc agccagtcac cgaagagtgc cgggcgccgg
      661 gggggaggga tacgtcctga ttgaaaagac gcccgattgt actccgagtg cgcaggattt
      721 caacgagtat ttgccctctc gcttgacaaa cccgtgcggc agcttccaag ctcgccctgg
      781 aaggaggcat cggagacttg gacccggatc gtatcgacta tccacggatc gtgcaggacg
      841 ccctgaggga cgccgtccgc cgcgtgctgg agcaggcggc cgagcacggc gtgccggggg
      901 agcaccagct ctacatcgcc ttccgcaccc agcaccccca ggcgaaggtt ccccccttcc
      961 tccgcgacca gtacccggag gaggtgacca tcgtcctcca gcaccagtac tgggacctcg
     1021 aggtcaccgc cgaagccttc tcggtgaccc tcaacttcaa cgccgtgcgg caacggctca
     1081 ccgtgccgtt cgccgccatg accgccttct tcgacccggc cgccgagttc gccttacgct
     1141 tcgagccgaa ccccggagag gccgccgcga cagaggctcc ggctgccgcg gagccggcgc
     1201 cggaggcgcc ccgccccgcc ccggccaagc cgggcgaggt gatccgcttc gacccctccc
     1261 gccgcaaata gcgccgcgag gtagcgccga aaaggcgagc gtcctgccgc ggcggcaccc
     1321 gcgtccccga aaccggtcgc cgcggtgcgg caggcggacc ggcccccggc gggccggtcc
     1381 gctgcaaacc attgttcccc agcgatttgg cgtctgtccg cacgggctcc ggggcggtgg
     1441 cattcccctt gctctttccc tggaatggca gggattgtga ggtaacgacg atgcgattca
     1501 cccagaaatt attccccgtc ctcctctgtg ggactgtgct gctggccgcc ggatgtggcc
     1561 gcgacgcgga gcaggaggag gcccgggttg tcggagagga ggccagccag tcccggcccg
     1621 ccgacgagga caagggctat gtgatcgagg aacccgggct gacctccgag atggcgaccg
     1681 ggaccgagat cgccgccgag ggcgagccgg aggccctcga agctcaggaa gagtcgcagg
     1741 aagccgccga gctgcggcgc gagacggtcg tcgacatcga ccaagggacg cgtccgtcgc
     1801 cgccggcggc gcgggagcag agccgccaga cccaggccgc ggagctcgaa gcccgcgagc
     1861 gccggctcga gcagcgccag gccgagctcg aagcccgcga gcgcgagctg agccggcgcg
     1921 agcggcccca gcggccggcg gctcgccctg aacgccccgc gccggccgag gagaccgagg
     1981 tgccggtgga gatcgaggag accgccgagg tcggcgagac ctcgcggccg gtggaggtcg
     2041 agcccgaggt cgaggcggac cccgaggagc gggcggtgcg ggagctcccg cggacggcgg
     2101 cggccagcgc caccgtgcct accggcacgc tgatggaggt cgaggtcctc gagcggctcg
     2161 ccagcgacac cacccgggcc ggcgagacct tccgcgcccg catcgtctcc gacctgcgct
     2221 ccgacgacgg ccgcgtggcc gtgcccgccg gctccgagat cgccggcgtg gtcgacgagg
     2281 tggtgcccct gcgcaaggtg ggcggccggg cgaagatggc cctgcgcttc accgacctgg
     2341 tgctcccctc cggcaccacg gtgccgatcc gcgcctccct cgtgcaggag gggcgcagcg
     2401 agaccaagaa ggacgccgcc accatcggcg gcgccacggc gggcggcgcc atcctcggcc
     2461 gcatcctcag caagcggggg gaccgctccc gcgccacggt gatcggcgcc atcatcggcg
     2521 ccgccgccgg cacggccatc gcctcccgtt cccccggcga ggaggtggtc atcgccgagg
     2581 ggaccgtggt cgacctgagc ctggatcagg acgtcgagat ccggccccgg cgctagcacc
     2641 ggggcttcca ctatagaggg gcgggcggtc ccatcccgcc cgcccctccc ttctcttccc
     2701 tcgactcata gagcgccggg aatagccagg atggcacggc ggggttatgc tcgtcattcg
     2761 cggtttcctt ctggaccgcc ccgggtgacc ccatgaccga tctctccgct ccctcctccc
     2821 gccggggtgc ccgtccgggc ctgttcggcg ggcaggtctc ctccaagcag cgcggcccgg
     2881 cccgcgatcc ttccgaagcg gcaccttccg agacggatcc gcagaagcag gacaagaaga
     2941 agccgaactt ccgggccttc ctgggcgacg cccgcgagct catccgggcg cgccgggggc
     3001 ggctcatcct cgggctcggc ctgatgctga tcagccggct cgccagcctg gtgctcccgg
     3061 gcacgagcaa gtacctgctc gacgaggtca tcggcaagca gcagcggcag ctcctcgtgc
     3121 cgctgatcct ggtggcgggg acggccaccc tgctccaggc ggtgacctcc ttcgccctgt
     3181 cgcaggtgct gggcaaggcg gcccagcggt cgatcaccga gatgcgccgc cgcgtgcagc
     3241 gccacgtcgg ccgcctgtcg gtccgctact tcgagcagac caagtcgggg gcgctgctgt
     3301 cgcgcgtcat gaacgacgcc gaggggttgc gcaacctggt ggggaccggc ctggtggaga
     3361 tcgtcggcgg cctggtcacc gcggtcctcg ccgtcggcat cctcttcttc ctgagcgcca
     3421 agctgacgct gatcgccatc gtcgtcctgt cgctgttcgg cttcgtcctg aagttcgcct
     3481 tcaagaacct gcggccgctg ttccgcgagc gctcgcggat caacgccgac ctcgccggcc
     3541 ggctcaccga gtcgttcaac ggcgtgcggg tggtcaaggc ctatggcgcg gagcggcggg
     3601 aggcgctggt cttcaccaag ggggcgcacc gcctgttccg taacgtcgcg cgcacgatca
     3661 ccggcttctc ggcgatcggc gccgcgtcgt cgctgctgct cggcgcggtc agcgtgtcga
     3721 tcatgctggt cggcacgcag gagatcctcg ccgagcggat gacggtgggc gacttcgtgg
     3781 ccttcaccct ctacatgggg atgctggtgg ggccggtggt gcagatcgtc aacatcggca
     3841 gccagatcac cgaggccttc gccggcctgg agcgcatccg cgagatccgc gctgagacgc
     3901 cggaggacga ggacgaggcg tcgcgccggc ccatggggcg gatcgacggc cgggtggagc
     3961 tgcgggacgt ctggttcgag taccaggcgg acgcgccggt gctcaagggg atctccttca
     4021 ccgcgtcgcc gggcacctcg acggcgctgg tcggcccgtc gggctccggg aagagcacgc
     4081 tgatcggcct ggtcgcggcc ttccaccggc cgaccgccgg cgccatcctg gtggacggcc
     4141 aggacctcgc gacggtgaag ctgcgcgact accggtcgca gctcggggtg gtcttccagg
     4201 acaacttcct gttcgacggc acggtgttcg agaacatcgc cttcgcccgg ccgcacgcct
     4261 ccgaggagga ggtcgtgcgg gcggcgcgca tcgcccgctg cgacgacttc gtgcagaagc
     4321 tgccgcaggg atacgagacg gtggtggggg agcgcggcgt caagctctcg ggcggcgagc
     4381 gccagcgggt ggcgatcgcc agggcgatcc tggccgatcc gcgcatcctg atcctggacg
     4441 aggccacctc gtcgctcgac agcgagagcg aagcggcgat ccaggagggc ctcgcggagc
     4501 tgatgaaggg gcggaccacc ttcgtcatcg cccaccggct gtccacggtc cggcgggcgg
     4561 acacgatcat ggtgctggag gggggcgaga tcgtcgagcg cggccggcac gaggagctgc
     4621 tcgccctggg cggccggtat catgccctct acacccggca atacaacctg gagtcgaacc
     4681 tcttccgcaa cccgggcgag aaggtcgagg aggaggaaga ggccaagccc gcggtcgtcg
     4741 ccgacgcctc ggtcggccgg ttaccgctgc tggggtaggc gccctcaccc cccggcccct
     4801 ctcccgcctc cctcccaacc tccccgggag agggggaagc ggacaaaatc cttcctagat
     4861 gttgggggta actcccggcc acaaggggga ggtggtgcct ccccccctgg gcgggtgggc
     4921 ggcagggagt gggggaggtc aggagggggg ttttgcaacg ccgcgctcca cccccacctc
     4981 gtgcctacgc ccctcggacg cctgaatcgg gcgaggctct tccaacgcaa tcccaccaaa
     5041 ccgctctggt caattggaag gttgtactcg ggacagtgcc agtgggccac tccgtcgtcg
     5101 caatcccacc aaaccgctct ggtcaattgg aagggcgctc atcacgatcg ccatgcgtca
     5161 acacaatgat ccaggtcgca atcccaccaa accgctctgg tcaattggaa gcaccccgca
     5221 ttcggatgtc ccatcagatg atgacggccg acgtcgcaat cccaccaaac cgctctggtc
     5281 aattggaaga gatggagagg atccgacaag agttcctcct gtaccaaaga gtcgcaatcc
     5341 caccaaaccg ctctggtcaa ttggaagccg attttcactt ccacgacctc cggggcatct
     5401 gggcggtgtc gcaatcccac aaaaccgctc tggtcaattg gaagcatact gtccctcaac
     5461 tacgggacga acttcataga aagtcgcaat cccaccaaac cgctctggtc aattggaagc
     5521 cgatcattta catcccatac tcggatgccc gctatccgtc gcaatcccac caaaccgctc
     5581 tggtcaattg gaagaccgtc cttgaagcga agctgcggat ggacatcaag aagtcgcaat
     5641 cccaccaaac cgctctggtc aattggaaga atccttggaa gcgtgaagag ttggcgctct
     5701 tcccactagg tcgcaatccc accaaaccgc tctggtcaat tggaagggcc gcctttgcaa
     5761 ccccttccaa cgtcgcgact taacccccct cccgccgtta cccctgtttt cgggcctcgg
     5821 aaccgcctcc gatccaaggt cgagacacac cgctcccgcc tccaacccct ttctatcaaa
     5881 catttccgtc tccgccgtta cccctgtgcc aaaatggcga aatggggcag gcatgattct
     5941 tgtcgggtat ggcctttgct tgaaaatccc acagtactga tctgggcaat tgggttgtca
     6001 aagagccgcc gcgaggcgtc gaggctcgcg ctcctggagg ttatgacacg gttccgggcc
     6061 gcaggggtga gaggggacgg ggaaaagaag aagaaaaaga agaaaacccc cctcctggcc
     6121 tcccccgctc cctaccgccc gccctcccag ggggggagga accacctccc cttgggaccg
     6181 cagggtagtg ccagaggcga ggtgaggagg aaccacctcc ctgggaacca caaggtagcg
     6241 gcgcggggag gcgccgccgg gaggacaggc cccccggcgg cggggtgggt caacgtcaga
     6301 cttcgtggta gtccagggtg ccccggtcag ggtcctggac gacgcggagg acggtccagc
     6361 gacggtccgg ggggaggttc ccctcctcca tgggacgcca gacgacgaac cagcgtccga
     6421 agaggtggtg gaccgccagc ttcgcctggt ccggcgtcac ggccggcggc tgccaggcgt
     6481 cggggtccgc cttggccttg tcccgctcct cgggcgtgcc ggtgaagtgg gtggccaccg
     6541 cggcgtcgta cagcttgcgg cgggtctcct ccgtctcctc ggtcaggtcg atgtcgggac
     6601 gggacgcagg atcggacggg ggcgtgggtg taccatcagg acgggtgttc atggagttta
     6661 gagctccttg tgagcaccta ggctccgggt gaaggggcct aatccttcac tcggggccgc
     6721 tcggtgctga ttgccgcggg agacattcca cgcggtccag catgggacaa caacgacgag
     6781 caggattgta gcgcaaagtt ccgggcggat gccagcctct cgacgcatcc gcccggtcct
     6841 tatctccccc acgccctccc cccggtctcc tcacgcgcga tgtgttctcc ctcggcgcgc
     6901 acggcgacgg cgacgagccg aacgtcgcag agctgctttc aaacgtcgca gagtcgctcc
     6961 ggatcatcgc gtggcggcgt tcgttcgtcg cagatcaacc ttcaatcgtc gcagatgaac
     7021 gttcgttcgt cgcagatctg tgcgcattcg tcgcagagtt gtgcggatct gcgacgaagc
     7081 agcgtggctc tgcgacgttc tccgcgggtc gaggcccgcg ggaacgctgc tctgcgacgg
     7141 cctcggcctc cagggcgtcg gcggacttct tgcgcagctc ctcgggatcg gtcagctccg
     7201 gcgacagcgc gaggagggcg gccaggtgct cgagcaccgc cgggtgctcg ccgttgcggg
     7261 cggccccctc ggcctggatg tagagctcca tggcgcggcg ggcgcggccg aagtcggcct
     7321 gggcctcggg agacagggcg gccaccagct ccggctgcgc gatgccggtg tccaccgccg
     7381 agcgcagccg cgccaggttt ccgtccaggc cggcggccag gtcgtcgggg aggcggtcgc
     7441 gggcggcgag gagcagcgtc tcccgcaggc cgtcgagcag acggcaggcg gacggcggca
     7501 gcgccgcctc gtcggcggcc gtgaaggtgg ccaggatctg ccgcgcgcgg tcgtcctccc
     7561 cctcggccac cagcaggcgc gcctgttcca ggcgctcggc ggccggcggc tgcggcgggc
     7621 gggcggacac gatcatggtg ctggaggggg gcgagatcgt cgagcgcggc cggcacgagg
     7681 agctgctcgc cctgggcggc cg
//
LOCUS       DASPJI010000010         8894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_401510, whole genome shotgun sequence.
ACCESSION   DASPJI010000010 DASPJI010000000
VERSION     DASPJI010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8894)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8894
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401510"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..2514)
                     /locus_tag="VEL74_00240"
     CDS             complement(79..2514)
                     /locus_tag="VEL74_00240"
                     /inference="COORDINATES: protein motif:HMM:NF017850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HYG60981.1"
                     /translation="MDSALQLAPAERLFAELVLTALAAERRLRPYEGELASDPMLLRS
                     FGTFGKWQEIPHPSRFVRERSEDGDRVGELYTRMVETDANIAALWEKRVKAVLALPRT
                     IRAADSSPEAEEIALFVHTALSLIPLRVVPISNTLEAVLKGVAFNEILWERATRGPLA
                     GAWLPVDVIDRPMWRFGWSATDKSLHVVTNPGRIAPIPAPPMKFQVLSYGTRDNPWGR
                     ALLDRLYWVWYLKRHSSKYWALFVERFAQPLVKGTYPYKPDQDLLNKANEDRLLAILN
                     TIRTGSSIALPEGLDVAFLEASRGGDASYFSFVSWLERAQALLLLGEVDTSGLARGAG
                     SFAKALISNEVRLETVQHDAHLMGSFESDTLIRWMVLLNFGPDAPIPKSIYDATDAAD
                     RRQRMQGIAKALNDGVSVPLGYYRMTMRVPAPLDREPVVDHQPIREDVAPEFRAPQPL
                     AFLSSPLSQEDEAHGALVEARDHQFELVAAHFAPALTGHFASQLDRLLVLLESETSSP
                     LDRLVAGETPATVIDAIATAQLHAAGLALLHAREDLGALRLTAPADWQSARTPSAAIA
                     FWAAHLGLSQDFFLTLSDSARRIASASAGLAEGPLLIDLHEVLERAPATGLDRTTAHR
                     MLRETYEAHGLLPASPHHADLVLSNTVQQSAHAVRYLQTVGNPAASRLIPYLVWWTVG
                     DDRVRERPYHNHAVMHGRVFAIDHPIWKTWWPPAGHNCRCGIGTLTVAEARRRGLVGS
                     EPTGPWPIAPGTGGLALPDPGFRGAPDLATEAERLATQSRRIHRDAQVQGGDLLAAIT
                     RLFTALALLPEVP"
     gene            complement(2518..3621)
                     /locus_tag="VEL74_00245"
     CDS             complement(2518..3621)
                     /locus_tag="VEL74_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60982.1"
                     /translation="MKRTQFVRVVLAVLAVLGLFGVLPPAEAQIPRPNKAPYNTSQLR
                     QYVTSAAFPWTITVCKGAPYGGDYSDLSEALDAIPKRFPTRGPMQRVLVLVHPCFVSS
                     PTSFSYEETTLSVPSWTTLQGVPTGASGTVDAQSARVFLKLTGTNTTCAGCPSALLKL
                     DTGTSLINFFVLSGTPPTGPIRLVEASGFVTLTNVVLQLTGTETHPVDLLYVTGGGVL
                     AHNFGLTRNTFSSLSRGLVTAGSGSATINGGRIFVPSGVPVENLGTGTIRLTGVHIEP
                     STNVDLKRSSTGTIETFSTDYATEAGGVIDRPIRTDALTLDNACRILTGTGTPEGVVA
                     APVCSLFLRQDGTAATTLYVKTSGTGNTGWTAK"
     gene            complement(3624..5567)
                     /locus_tag="VEL74_00250"
     CDS             complement(3624..5567)
                     /locus_tag="VEL74_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60983.1"
                     /translation="MHSVELLHPALPDLIDALLRRNRWSWQALERGDLKLGDQVLDVD
                     RLQWWLVNADPVLWVETNLVNKPEDGGGLWQLFPYQRPSLRFRGHVVHQDGAEVGKSR
                     EIVGLLLWGCLGAERGSVLVGSALDGDLDEIWEEVEWQLSANPFLANSLRARTTKPYR
                     RLTWRNGLRVLFRPAGHDGRAYRGIHVRGWLLHDEAAKVVNPRSWSEFWRAAKPGCEI
                     RIYSVPTGDRQCTFQRIADNAIPADSIVPADTPELVLAQLLRSTSETVPESTRALARD
                     LGGRVWVRFHWPKTIMPHPFWSEERRQEFVGFYGGPDEPGYVHNVLGLPGDPEYSLFP
                     ERLLKPALTWIPDYLSVSFRWDSRAGQVDGLSRRLNPSYPWGDEGELQPPAGDDLDEA
                     DGGTDIPQPYLTVLQEQADVSGWDRWDAEQRRQVVTDLTRFFVRHLPGDLTAGVDVGS
                     ATVTEILFERRSPLSDALVLRIHLTGWNWYAQRDFILALDAILKPAGGWGFDATGVGR
                     VLVDLLAPEFGDRLSGYVFNRSTPAIDPATGEALLDPASGREVLISYKELGTQLIERR
                     LHGRGLELPFDPEVRHLLANHTYSQSGDTRRFSKLNDHLVDALRVAELRKLTATWSVS
                     TPPLVYATPTANPLDSLDILRRM"
     gene            complement(5560..6156)
                     /locus_tag="VEL74_00255"
     CDS             complement(5560..6156)
                     /locus_tag="VEL74_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60984.1"
                     /translation="MSSKERAQRRAAAVKTGEHAATALGQALPPCKPAVCPNGGTGTC
                     DVKRAVESRGGGLSACLVSLGHHDVMQAYLAAIQSGDLAGLAELAATSMAGQLALAHG
                     ELSTLMHEGLVVNVPIVGRNADGDLEVVAERPVENPRAANTFQLLRQLGHTAADQAIT
                     PRSSGERERDQGLGLAGRAAWVSQMRKGLAGEGDDSDA"
     gene            6218..7348
                     /locus_tag="VEL74_00260"
     CDS             6218..7348
                     /locus_tag="VEL74_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HYG60985.1"
                     /translation="MTALVFREQVDSMSTRAQIGEAQETGAKRKKQRELGGQEERQNR
                     KDEAYVLNGWTSFLVSVRGRTEGTAGRYRRLVERLLADVGKPIAQLGREDIERHLRRL
                     HVAGRGESVRQGVVVAVRSLGEWCLAHGIVEVNPGAGLAGPSGFRREVRPLTVEEVSR
                     LIWGDRPGYLPRGHRKLRDRALLAVIYIAGLRASEPGPIRLSDVQWDETAGVFRLLLV
                     KSKAARREKWVHLDRQVSRVLGAYLAVRPAGSPWLFVSERGKPLTRAGVYQIFQRRTK
                     EEGIEARGRRMSPHILRHSIATHLLARGWDIRTVQIHMRHASIKTTEVYLHADEGRVQ
                     RMMLRWSPLGPRRERRVELGRGLSELLGELGVGRERVPGDEV"
     gene            complement(7345..7512)
                     /locus_tag="VEL74_00265"
     CDS             complement(7345..7512)
                     /locus_tag="VEL74_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60986.1"
                     /translation="MQKEHFAALAAGRPWQAGMVLVRGYWSFWSAVVAQLPISLARRV
                     YEVWKATKTGS"
     gene            complement(7731..8057)
                     /locus_tag="VEL74_00270"
     CDS             complement(7731..8057)
                     /locus_tag="VEL74_00270"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HYG60987.1"
                     /translation="MNFSRKIKDMDIPRLSSKEAIILQLLISNGEMYGLEMVGAAPDK
                     IKRGTVYVTLGRMAEKGYVESRQVPGDEGTGGLPRRLFQATGYGARVLHAWELASSTL
                     AVGAIP"
BASE COUNT         1497 a         2455 c         3490 g         1452 t
ORIGIN      
        1 aagccagctc aaggaggtga tcggcggcgg gggtccagag gacggagccg ctgatgatgc
       61 gggggttcgg cgcgggcttc atggcacctc cggcaggagc gcgagcgcgg tgaagagccg
      121 ggtgatggcg gcgaggaggt ctccaccttg gacctgggcg tcgcgatgga tgcggcggga
      181 ctgcgtcgcc aggcgctcgg cttcggtggc gaggtccggc gcgccgcgga agccgggatc
      241 cgggagggcg aggcctccgg tgccgggtgc gatgggccag ggaccggtgg gctcggagcc
      301 gacaagccca cggcgccgag cttcggcgac ggtgagggtg ccgatgccgc accggcagtt
      361 gtgaccggcg ggcggccacc aggtcttcca gattgggtga tcgatcgcga agacccggcc
      421 gtgcatcacg gcgtggttgt ggtacggcct ctcgcggacg cggtcgtcgc cgaccgtcca
      481 ccagacgagg taggggatca gccggctggc tgcggggttg cctacggtct gtaggtacct
      541 gaccgcgtga gcggactgct ggacggtgtt ggagaggacc aaatcggcgt gatgtgggct
      601 ggccggtagg aggccatggg cttcgtaggt ctcgcggagc atcctgtgag ccgtcgtccg
      661 gtcaaggccg gtggccggag cgcgctccag gacctcgtga agatcgatga gcagcggtcc
      721 ctcggccagt ccggcggagg cagaggcgat gcggcgggcc gagtcggaaa gggtcaggaa
      781 gaagtcttgc gagaggccca ggtgggcggc ccagaaggcg atagcggcag agggcgtccg
      841 tgcgctctgc cagtccgcgg gggcggtcag gcgcagagct cccagatctt cccgcgcatg
      901 gagcagggcg agtccggcgg cgtggagctg cgcggtcgct atcgcatcga tgaccgtcgc
      961 gggagtctcg ccggcgacga gcctgtcgag ggggctggac gtctcgctct caaggaggac
     1021 caggagacgg tcgagctgag aggcgaagtg gcctgtgagg gctggagcga agtgggcggc
     1081 caccagctcg aactggtggt ctcgggcctc gaccagggct ccgtgggcct cgtcttcctg
     1141 agacagagga gaggacagga acgccagagg ctggggagcc cggaattcag gggcgacgtc
     1201 ttcccgtatg ggctggtgat ctacgacggg ctcacggtcc aggggcgcgg ggacgcgcat
     1261 ggtcatgcgg tagtagccca gggggacgga gactccgtcg ttcagggctt tggcgatgcc
     1321 ttgcatgcgc tggcggcggt cggcggcatc ggtggcgtcg tagatggatt tcgggatggg
     1381 agcgtcggga ccgaagttca gcaggaccat ccaacggatg agcgtgtcgg actcgaagga
     1441 gcccatcagg tgggcgtcgt gttggacggt ctcgaggcgc acctcgttgg agatcagcgc
     1501 cttggcgaag gagccggcac ctctcgcaag accgctggtg tcgacctcgc cgaggagcag
     1561 cagggcttgc gcccgctcca gccaggagac gaacgagaag tagctcgcgt cgccacctcg
     1621 ggaagcttcg aggaaggcaa cgtcgaggcc ctccggcagg gcgatggacg aaccggtccg
     1681 aatggtgttg aggatcgcaa gcaggcggtc ctcgttcgcc ttgttgagga ggtcctggtc
     1741 cggcttgtag ggataggtcc ccttcacgag gggctgcgcg aagcgctcaa cgaagagcgc
     1801 ccagtatttc gaggagtgcc gcttcaggta ccagacccaa tagagccgat ccagcagagc
     1861 ccggccccac ggattgtctc gggtgccgta ggacagaacc tggaacttca tgggaggcgc
     1921 cgggatcggt gcaatgcgtc ccgggttggt gacgacatga agggacttgt cagtcgccga
     1981 ccagccgaac cgccacatag ggcgatcgat cacatcgacg ggcagccagg cgccggccag
     2041 ggggccgcgg gtggcgcgtt cccaaaggat ctcgttgaag gccacgccct tgaggaccgc
     2101 ttccagggtg ttggagatcg gcacgacgcg gagcgggatc agggagaggg cggtgtgaac
     2161 gaagagggcg atctcttcgg cctcgggcga cgagtcggcc gcgcggatgg tccgcggcaa
     2221 cgcgagcacc gctttcactc gcttttccca gagggctgcg atgttcgcgt cggtctcgac
     2281 catccgggtg tacagctccc cgacgcggtc tccgtcttcg gagcgctcgc ggacgaagcg
     2341 ggaggggtgg gggatctcct gccacttccc gaacgtgccg aacgagcgga ggagcattgg
     2401 gtcgctcgcg agctcgccct cgtaggggcg gagccggcgc tcagccgcga gagcggtgag
     2461 caccagctcg gcgaagagcc gctcggcggg ggcgagctgg agggcggaat ccatgggcta
     2521 cttcgccgtc cagccggtgt tgccggtgcc gctggtcttc acgtagaggg tggtcgccgc
     2581 ggtgccgtcc tgccgcagga agagcgagca gacgggagcg gcgacgactc cttcgggggt
     2641 accggttccg gtcaggattc ggcaggcgtt gtccagggtg agcgcgtcgg tgcggatggg
     2701 gcggtcgatg acgccgccgg cttccgtggc gtagtcggtg gaaaaggttt cgatcgtgcc
     2761 ggtggagctg cgcttcaggt cgacgttggt ggacggctcg atgtggactc cggtgagccg
     2821 gatggtccct gtgcccaggt tctccacggg aacccccgaa gggacgaaga tccggccgcc
     2881 gttgatggtg gcggagccgc tgccggccgt gacgaggcct cgggagagcg aggagaaggt
     2941 gttgcgggtg aggccgaagt tgtgggccag gacgccgccg cctgtgacat agagcaggtc
     3001 gaccggatga gtctcggtgc cggtgagctg gaggacgacg ttggtgaggg tcacgaagcc
     3061 ggaggcttcg accaagcgaa tggggccggt gggaggggtg cccgatagga caaagaagtt
     3121 gatcagagag gtgcccgtgt cgagcttcag cagggccgac gggcagcccg cgcacgtggt
     3181 gttggtcccg gttagcttga ggaagacgcg ggcggactgc gcatcgacgg tgccgcttgc
     3241 gccggtcgga acgccctgga gggtggtcca gctcgggacg gacagcgtgg tctcttcgta
     3301 gctgaagctc gtgggcgatg agacgaagca ggggtgaacg aggacgagga cgcgctgcat
     3361 ggggccgcgc gttgggaagc gctttgggat ggcgtccagg gcttcggaga ggtcggagta
     3421 gtcgccaccg taaggagcgc ctttgcagac ggtgatcgtc caggggaagg cggcgctcgt
     3481 gacgtactgt ctgagctgcg aggtgttgta gggcgctttg ttgggccgcg gaatctgggc
     3541 ctcggcgggc gggaggacgc cgaagagacc aaggacggcg aggacagcaa ggacgacgcg
     3601 gacgaattgg gttcgtttca tgtttacatc ctcctcagga tgtccaggga atcgagtggg
     3661 ttggctgtgg gggttgcgta tacgaggggc ggtgtggaca ctgaccaggt ggcggtcagc
     3721 ttgcgtagct cggcgacgcg aagggcatcg acgaggtgat cgttgagctt ggaaaagcgg
     3781 cgggtgtcgc cggattgcga ataggtgtga ttggctagga ggtgccggac ttcggggtcg
     3841 aagggaagct cgaggccgcg gccgtgaagg cggcgctcga tcagttgggt acccagctcc
     3901 ttgtaggaga tgagcacttc gcggcccgag gccgggtcca gtagggcttc gcccgtcgcg
     3961 gggtcgatgg ccggcgtgga gcggttgaag acgtagccgg agaggcggtc gccgaactcg
     4021 ggcgccagga ggtccaccag cactcggccg acgccggtgg cgtcgaagcc ccagccgccg
     4081 gcgggcttga ggatcgcgtc aagggcgagg atgaaatcgc gctgcgcgta ccagttccag
     4141 ccggtcaggt ggatgcgaag gacgagcgcg tcggacagag ggctgcggcg ctcgaagagg
     4201 atttcggtga cggtggcgga gccgacgtcc acgccggcgg tgaggtcgcc ggggaggtgg
     4261 cggacgaaga agcgggtcag gtccgtcacg acttgccggc gctgctcggc gtcccagcgg
     4321 tcccaacccg agacgtccgc ctgctcctgg aggacggtga ggtagggctg cgggatgtcg
     4381 gtgccgccgt cggcctcgtc gaggtcatcg ccggcgggcg gctgcagctc gccctcatca
     4441 ccccaggggt aggaaggatt cagccggcgg gagagaccgt cgacctggcc ggcgcggctg
     4501 tcccagcgga acgagacgga gaggtagtcg gggatccagg tgagcgccgg cttgaggagg
     4561 cgctctggga agagggagta ctcgggatct ccgggcaagc cgagaacgtt gtggacgtaa
     4621 cccggctcgt cgggaccgcc gtagaagccg acgaattcct ggcggcgctc ttcgctccag
     4681 aacgggtgcg gcatgatggt cttgggccag tggaagcgga cccagacccg gccgcccaga
     4741 tcgcgggcga gagcgcgggt gctctcggga acggtctctg aggtggagcg gaggagctgg
     4801 gcgaggacca gctcgggggt gtcagcgggg acgatcgagt ccgcggggat ggcgttgtcg
     4861 gcgatccgct ggaaggtgca ctggcgatca ccggtgggca cggagtagat ccggatctcg
     4921 cagcccggct tggcggcgcg ccagaactcg ctccaactcc tggggttcac gaccttggcg
     4981 gcttcgtcgt gcaggagcca gccgcggacg tggatgccgc ggtaggcgcg gccgtcgtgg
     5041 ccggcgggcc ggaagaggac gcgcaggccg ttgcgccagg tcagacgccg atagggcttg
     5101 gtggtgcggg cgcgcaggga gttggcgagg aaaggattgg cgctgagctg ccactcgacc
     5161 tcctcccaga tctcgtcgag gtcgccatcc agggcggagc cgacgagcac gctgccgcgc
     5221 tcggcaccca ggcaacccca gagcagcagg ccgacgatct cgcggctctt gccgacttcg
     5281 gcgccgtcct ggtggacgac gtgcccacgg aagcggaggg agggccgctg gtaggggaag
     5341 agttgccaga ggccgccgcc gtcctcgggt ttgttgacca ggttggtttc gacccagagg
     5401 acggggtccg cgttgacgag ccaccactgg aggcggtcga cgtcgaggac ctggtcgccg
     5461 agcttgaggt cgccgcgttc gagggcctgc caggaccagc ggttgcgccg gaggagggcg
     5521 tcgatcaggt ccgggagagc ggggtggagc agctcgacgc tatgcatcgg agtcgtcccc
     5581 ctccccagcg aggcctttcc gcatctgact cacccaagcg gcccggccgg caaggccgag
     5641 accctggtct ctctcgcgct cgccgctcga gcggggcgtg atggcttggt cggcggccgt
     5701 gtggccgagc tggcggagaa gctggaaggt gtttgccgcg cgggggttct cgacagggcg
     5761 ctcggccacg acttcgaggt cgccgtcggc gttgcggccc acgatgggga cgttgacgac
     5821 gaggccctcg tgcatgagcg tggagagctc gccatgggcg agcgcgagct ggccggccat
     5881 ggaggtggcg gcgagctcgg ccaggccagc gaggtcgccg gactggatgg cggccaggta
     5941 ggcctgcatg acgtcgtggt gaccgaggga caccaggcag gcggagaggc cgccgccgcg
     6001 tgactccacg gctcgcttga cgtcgcatgt gccggtgccg ccgttcgggc agacggcggg
     6061 tttgcagggc ggcagtgcct ggcccaacgc ggtggcggcg tgctcgccgg tcttcaccgc
     6121 ggcggcgcgg cgctgcgcac gctctttcga ggacaagggc ttgcccttgg gccgaccgcc
     6181 ctttcctttg ggttcctgct gtgaccgatt cggcatcttg acagcgctcg tgttccgcga
     6241 gcaggttgat tccatgagca caagagccca aatcggagaa gcccaggaaa cgggggctaa
     6301 acgtaagaag cagagagagt taggaggcca ggaggaaagg caaaatcgta aggatgaagc
     6361 ttacgttctc aatggatgga cctctttcct ggtgagcgtc cgagggcgga cggaggggac
     6421 ggcggggaga tatcggcggc tggtcgagcg gctgctcgcg gacgtcggga agcctattgc
     6481 gcaactcggc cgagaagaca tcgagaggca cctgcgccgg ctgcatgtgg ccgggcgggg
     6541 ggagtcggtg cggcaagggg tggtggtggc ggttcggtcg ctcggggagt ggtgcctggc
     6601 tcatgggatc gtggaggtga acccgggggc ggggttggcg gggccttcag gcttccgaag
     6661 ggaggtacgg ccgctgacgg tggaggaggt gtcgcggctc atctggggag accgcccggg
     6721 ctacctgccg cgcgggcatc gaaagctgcg ggaccgggca ctgctcgcgg tgatctacat
     6781 cgcgggcctc cgtgcctccg aaccggggcc gatccgcttg agcgacgtgc agtgggacga
     6841 gacggcggga gtctttcggc ttcttttggt gaagagcaag gcggcgcgcc gggagaagtg
     6901 ggtccacctg gaccgtcagg tgtcccgggt gctcggtgcc tacctagccg tgcggccggc
     6961 gggctcacca tggttgtttg tctcggagcg gggaaagccg ctgacgcggg cgggggtgta
     7021 tcagatcttc cagcggcgga ccaaagagga ggggatcgag gcgcgagggc gaaggatgtc
     7081 gccgcacatc ctgcggcact caatagccac gcacctgctc gcgcgaggat gggacatccg
     7141 gacggtccag atccacatgc ggcatgcttc gatcaagacg acggaggtgt acctgcatgc
     7201 ggatgagggg agggtgcagc ggatgatgct ccgatggagc ccgctcggac cacggaggga
     7261 gcggcgggtg gagttggggc gagggctgtc ggagctgctt ggggagctgg gggtgggtag
     7321 ggagagggtt cccggagatg aggtttagct tccggtcttg gtagccttcc agacctcgta
     7381 gacgcggcgg gcgagggaga tcggcagttg ggcgacaacg gctgaccaga acgaccagta
     7441 accccgaacc agcaccatgc cggcctgcca ggggcggccg gcggcgaggg cggcgaagtg
     7501 ctccttctgc atgtcgctga gagtgggctc cagaactcgg gtgaaggttc tctttgagaa
     7561 gcagagctca gcgcaacgtc ggaggcccca accgggagct cgggatacgg gaggacggag
     7621 gagtcgcacc atccggagga tcacgcgaaa aggagctgcc agcagttcaa gaaggactcc
     7681 caccagaagc accacaagca gtccaaggag gtacatcggg cccgaaggat tcatgggata
     7741 gctccaacgg cgagggtgct gctcgcgagt tcccaggcgt gaagcacacg agcaccgtag
     7801 ccagtggcct gaaagaggcg ccgaggcagg ccgcctgtgc cttcgtcgcc cggcacctgg
     7861 cgcgattcga cgtagccctt ctcggccata cggcctagag tgacgtagac ggtgccgcgc
     7921 ttgatcttgt cgggagcggc gccgaccatc tccaagccgt acatctcgcc gttagagata
     7981 aggagttgga ggatgatggc ttccttagac gagagtcggg ggatatccat atctttaatc
     8041 tttcttgaaa agttcacttg gagcttctgg cctgccagag ggctgcgatg cgacctagga
     8101 cggagaagcc gagttgactg acggcagccg cggcgagcgc catacaccct tgtaggaaga
     8161 tccggcgggc cttcaacggg tgcccttcgg cgagggcggc ggagtgttct tgacaaaggt
     8221 ccgcgagggt tggttcgagg acttgggcgt aaagcagagg agccaaggcc tggagacggc
     8281 ggcggaggcg ttccagaagg ggcgctcgcc tgttggtggt ctgcgacaaa gctggcgcag
     8341 gagaccctgg actaggcatc ggggtggatg ttttcggagc gtcgggggac aggtggggca
     8401 actcctggtg tctcttgtcg cgtattttct ccaggagcct tttgtttagg taggaagtat
     8461 gaaagcacac ggcgtttctg ctgatctgta actccctact aatgtcatcc acacttttgc
     8521 cctgaaggta gagcctaaac acctgccgtc tccggggagt aaggatcgca tgaatgtcat
     8581 tcaaggcatt aatgaaatct ggactaatct ccagcgcggg cccggctttc tgtgggagcg
     8641 gctccatatc actatgctga cagagtgtag gactgctgtc aagtaggagt ttagtcaaca
     8701 aaagtcctgt tctgctgact gccgtggccg gcttggtgcc ttggcgtaag tgtctgacgc
     8761 ggctgtagtt gacggcgctg atgcgtggcg gaacccctcg cgccggcggc gccggtgcgg
     8821 aaaacgaaac cagtttcgtt ttcggcgggc gcaaaggccc gtcagcgctg cgttccgccg
     8881 gatcggcggg gcgt
//
LOCUS       DASPJI010000011         9485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_572672, whole genome shotgun sequence.
ACCESSION   DASPJI010000011 DASPJI010000000
VERSION     DASPJI010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9485)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572672"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1590
                     /locus_tag="VEL74_00275"
     CDS             <1..1590
                     /locus_tag="VEL74_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYG60988.1"
                     /translation="FFASRREHHELAVDLLMRLAGSDPGGGHEAAAFLASERAHARGL
                     LDLMAEGRIGAGRELPRALREREVEIDARLSQVQNLLIRELSEERPDPTKVGKLRREL
                     RQAEEQRQDLEARIRREHRRYAQARYGEPLPLSRIQPLLDAETALLEYVIGQEASYLF
                     AVTREGMTTHRLPPAGEIARLVRAARAGLEAPDSPRRLRSYAAAARGLHDRLIAPARR
                     TLAGKRHLLVAADRSLHLLAFETLLSADAAGRPAHELPYLAREYAVTYVPSATVWAEL
                     MESRRQRPPPDPSAKRLLAVADPLNLSKAPAPPGQQGAAPPAGVTRTGRGLLDADRWS
                     WSRLEGSGREVQRIAGLYPRGDVALYIGAEATEENLKGSRWLSDAHRLHFATHGIISE
                     TQPELSALQLTRAEGSREDGLLQAYEIFDLRLGADLVVLSACETALGKQVTGEGLMGL
                     TRAFLHAGAASVVVSLWPVADDSATPDLMVSFYRRLDRSRDAARRKAEALQAAKLDLI
                     REKQYAHPYYWAPFILVGEPE"
     gene            1679..2161
                     /locus_tag="VEL74_00280"
     CDS             1679..2161
                     /locus_tag="VEL74_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60989.1"
                     /translation="MHNGQIHVYVEPTDGNHMVPSHVGLDSASIATGALSVGMGAVDA
                     AGETLAAVAVPVLSSPALAGLGLETLAVVVRSDFFHYGFLFFGLVSLVLNATNLFLGF
                     CLKGKNRSLSAEVAKTYEAKTHGTPDHGEGHSHEHEHGHEHEPAPAQPVKAPFTPRAS
                     "
     gene            2243..3004
                     /locus_tag="VEL74_00285"
     CDS             2243..3004
                     /locus_tag="VEL74_00285"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HYG60990.1"
                     /translation="MKKIWIGTAALVLAALPLMAHEEGGMVSADVVPVLSVQGSGQTR
                     VEPDEAVVRLGVTAQAPTARAAQDQVNKTAAAILAAVRALGVAAEQVQTSELSLGPVY
                     SSGGPQERNEPRITGYQANNVVSVTLAELNRVGPVIDAGLGAGANRLEGVMFGLRNDA
                     PARAAALANAVTEARGKAEALARAAGVRLVEIVEIVEGGVSVFTPTMARGGRVAMEAM
                     DMAMATPVSSGQVSVDASVTLRYRIAPQAGAGAPR"
     gene            3013..3444
                     /locus_tag="VEL74_00290"
     CDS             3013..3444
                     /locus_tag="VEL74_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYG60991.1"
                     /translation="MNAEEVVRLLGLAPHPEGGFYRETFRDGSPGTGRGASTAIYFLL
                     REGEVSAWHRIDAAEVWHWYAGAPLELTLSPGRGHVTVRLGPDLAAGERPQAVVPAGE
                     WQSARTLGAWALVGCTVAPAFEFSTFEMAPPGWAPEPEVGA"
     gene            3444..3950
                     /locus_tag="VEL74_00295"
     CDS             3444..3950
                     /locus_tag="VEL74_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG60992.1"
                     /translation="MRWRWLLAALALAACEPREVPGAAGEMPAESPEAVRQARERREI
                     RQKSGCVAPWPSGAREAVESVQKAAAAGNAAALRPFMASELTWSFGGDADAGQAIKHW
                     RQNPEVLREMAAILARGCVVQETPEGASERIVCPPEYESQSGYLGYRAGFERRGEVWK
                     MTFFVQGD"
     gene            complement(3960..4814)
                     /locus_tag="VEL74_00300"
     CDS             complement(3960..4814)
                     /locus_tag="VEL74_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYG60993.1"
                     /translation="MATSLATDQTAKGNSSSGEHLSEETADFYRRTMRKLEQAGVRFL
                     VGGAYAYARYTGIVRHTKDFDIFVHHRDFDQALDVLAKAGYQVERTFPHWLGKAYEGD
                     AFVDLIFSSGNGIAEVDDLWFEKAVDEEVLGVPAKLCPAEEIIWSKSFIMERERFDGG
                     DISHLIRSCAESLDWDRLLQRFAEHWRVLLTHLLLFGYIYPGEAHRIPARVMEDLLGR
                     ALGEVRDPSLLQGVEGLCRGTVLSRSQYLKDVEEWDYRDAREKPVGEMSAKDIEVWTE
                     AAREEGVA"
     gene            4924..5736
                     /locus_tag="VEL74_00305"
     CDS             4924..5736
                     /locus_tag="VEL74_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYG60994.1"
                     /translation="MSRRDGTVRIGALADLHYTRTPEQPLRELLSQAGRESDVLLICG
                     DLTDHGLPEEAQGLAREIHGAVSIPVIAVLGNHDFEGGQQEEVKTILRDSGIRVLDGD
                     ATEVEGVGFAGVKGFAGGFGKRALGPWGEDIIKLFVREAVDEALKLEAALARLRKRPR
                     IAVLHYSPIQETVQGEPLEIYPFLGCSRLEEPLTRYPVDAVFHGHAHRGCHEGKTASG
                     IPVFNVSIQLLRQTYPDRPAFMTFEVNVDEEADPSQQRQPGEDRRVQERRKE"
     gene            5741..6046
                     /locus_tag="VEL74_00310"
     CDS             5741..6046
                     /locus_tag="VEL74_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HYG60995.1"
                     /translation="MPTAWSDKDERQYEHIKESSEERGVPEKRAKEIAARTVNKNRRE
                     EGRTPNKTTQGTGNPNKGLEARKVDELRNIASDLEIPGRSKMNKADLVKAIRGKRSH"
     gene            6101..7003
                     /locus_tag="VEL74_00315"
     CDS             6101..7003
                     /locus_tag="VEL74_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYG60996.1"
                     /translation="MESSEGFVFIRPGLNLYYQSVGQGPEAVVIPNANWLGHALQPLA
                     EGRRLIFYDPRSRGRSSTVTDPRQLGIEEDVNDLEAVRRFFGLERMALLGSSYHAAIT
                     ALYAMEYPEKVARLLLVCPITPRRPGEWAQETPTADMLVYPPGIPRLEEMRRDAVDRE
                     DPVAFCRAWFTHFLLPAQMGEPSAILRFPVEDVCSFPNEWPSHTMSLYFEHIIPKMED
                     WDFRPRLAGLETPLLVIQGTDDLVPLEASRDWAAYAPNAMFLPLAGAGHHPYLERPRE
                     FFSAADRFFKGEWPQGAQAVQHLA"
     gene            complement(6954..8774)
                     /locus_tag="VEL74_00320"
     CDS             complement(6954..8774)
                     /locus_tag="VEL74_00320"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYG60997.1"
                     /translation="MEGTVVRAEPGAGDVGDALRPRLHALAAAGNSVALLLLILALAL
                     GPRLSGDRAGFQPPPSPPSGPARAQRVLLVVIDGLRADHAFDATLMPQLAGLAHRGST
                     GTALVESLIPSTIAGIRTLAEGEVPPPASFLQDFGASPAPSGGIFAAARAAGLRTFAA
                     GPRLWADLYGSWLDGSLAVRTVSGEDHRVLAAGAAALAGGRYGLVVVHLSEPDDAAHL
                     HGARSPEHRQALTRADAALGRLLSLAGAGTAVVVTSDHGVTGSGGHAGPEPAVVRTPL
                     VVAGPGLPRGRFGAVRQSGVAALVLAPLGLPAPPVQPAERGTGWPVPLLALLAAGACL
                     LLWRAVAQGEIHRRAPFLLNATLWLSLAAAALGLHRLALAASLASLGWVAMAVRSGRR
                     GSLPWAAFAAGGLFGLLRLGEAWASATSGDLSAGGPAVLILAVLAGLGLGALLGRRLL
                     PRAPLLCGMLCGIAPAVLARGLGETASLSTLDVRLAFALADGPLGLPGAVAAAALRQA
                     LPALAVVFGLAAAFARSPQAGAFAAGLAAVLAGQAVAAALVLAGSAGYPAAGSLSLGL
                     LVRLVGETNALFLGAALAAAVPALVRRGAGRPAPPAATPP"
     gene            complement(8717..>9485)
                     /locus_tag="VEL74_00325"
     CDS             complement(8717..>9485)
                     /locus_tag="VEL74_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG60998.1"
                     /translation="ADADVRLTPGGLARGTAFLKASGAGLVSGFPRQETETLLERMLI
                     PLMHFVLLGFLPMARMRRSTHPAYGAGCGQLFFARRDAYERAGGHSAIAASLHDGIAL
                     PRAFRRAGFATDLFDATDAASCRMYTNAGEVWRGLAKNAVEGVASPGKILPVTALLLT
                     GQVLPPLLLLPALIKGDAVALGASLAGTLAVYLPRLAAARRFRQPLDGALLHPVSILL
                     FLAIQWTALTRHLLGRPAAWKGRSYGPSPAPETLETR"
BASE COUNT         1407 a         3335 c         3550 g         1193 t
ORIGIN      
        1 ttcttcgcct cccggcgcga gcatcacgag ctcgcggtcg acctgctcat gcgcctcgcc
       61 ggcagcgacc ccggcggcgg ccacgaggcc gcggccttcc tggccagcga gcgggcccac
      121 gcccgcggcc tcctcgacct catggccgag gggcggatcg gcgccggccg cgagctgccg
      181 cgggcgctgc gcgagcgcga ggtggagatc gacgcccggc tctcccaggt gcagaacctg
      241 ctgatccggg agctctcgga ggagcgcccc gacccgacga aggtcggcaa gctgcgccgg
      301 gagctgcggc aggccgagga gcagcggcag gacctggagg cccgcatccg gcgcgagcac
      361 cggcgctatg cccaggcgcg ctacggcgag cccctcccgt tgagccggat ccagccgctc
      421 ctggatgcgg agaccgccct gctggagtac gtcatcgggc aggaagcctc ctacctcttc
      481 gccgtcaccc gcgaggggat gaccacccac cggctgcctc ccgccgggga gatcgcgcgc
      541 ctggtccggg cggcccgcgc cggcctcgag gccccggact ccccacggcg gctgcgcagc
      601 tacgccgcgg cggcccgcgg gctccatgac cggctcatcg ccccggcccg ccggaccctc
      661 gccggcaagc ggcacctgct ggtcgccgcc gaccgcagcc tccacctgct ggcgttcgag
      721 accctgctga gcgccgatgc ggccggccgg ccggcgcacg agcttcctta tctagcgcgc
      781 gagtacgcgg tgacgtacgt gccctcggcc acggtctggg ccgagctgat ggagtcgcgg
      841 cggcagcggc ccccgccgga cccgtccgcc aagcggctcc tggccgtcgc cgaccccctg
      901 aacctctcca aggcgccggc gccccccggc cagcagggtg cggcgccgcc ggctggcgtc
      961 acccgcaccg ggcgcggcct cctcgacgcg gaccgctgga gctggagccg tctcgagggc
     1021 tccggccggg aggtccagcg gatcgccggg ctctatccgc gcggcgacgt ggccctctac
     1081 atcggcgccg aggccacgga ggagaacctc aaggggagcc gttggctctc ggacgcgcac
     1141 cggctccact tcgctaccca cgggatcatc agcgagaccc agcccgagct gtcggccctc
     1201 cagctcaccc gtgccgaggg atcgcgcgag gacggcctcc tccaggcgta cgagatcttc
     1261 gacctccggc tcggcgccga cctggtcgtg ctgtccgcct gcgagacggc gctcggcaag
     1321 caggtcaccg gcgaggggct gatgggcctc acccgggcct tcctccatgc cggcgccgcc
     1381 agtgtggtgg tgagcctctg gccggtggcc gacgactcgg ccacgcccga tctgatggtc
     1441 tcgttctatc gccggctgga ccggtcgcgg gacgccgccc ggcgcaaggc cgaggcgctc
     1501 caggccgcca agctggatct catacgagaa aagcaatacg ctcatcccta ttactgggca
     1561 cctttcatcc tggtgggcga gccagagtag acgcaggcgc gtaacatttg tggttccccg
     1621 ggcgcctgtc cccgctggaa ccgtccgtcc ggtgaatgtt ggagaaaagg agagagccat
     1681 gcacaacggt cagattcatg tctacgtcga gccgaccgac gggaaccaca tggtgcccag
     1741 ccacgtgggg ctggattcgg cgagcatcgc caccggcgcg ctgtcggtcg gcatgggagc
     1801 ggtcgacgcg gccggcgaga ccctcgccgc cgtcgcggtt ccggtcctga gctctcccgc
     1861 gctggcgggc ctcgggctgg agaccctggc cgtggtggtg aggtccgatt tcttccacta
     1921 cgggttcctc ttcttcggcc tggtgagcct ggtgctcaac gccaccaatc tcttcctcgg
     1981 cttctgtttg aaggggaaga accgaagcct ctcggccgaa gtggcgaaga cctatgaggc
     2041 caagacgcat ggcacgcccg atcacggcga ggggcacagc cacgagcacg agcatggcca
     2101 cgagcacgag ccggcgcctg cccagccggt caaggcgccg ttcacgccgc gggcgtcgta
     2161 gccgcgccga gacgtcccgg tccctcccgt gcttccgggc gacccgcata tactgcgggg
     2221 cggacgagaa agggaggaac cgatgaagaa gatctggata ggaaccgccg ccctggtttt
     2281 ggcggcgttg cctctgatgg cgcacgagga gggggggatg gtgtccgctg acgtggtgcc
     2341 ggtgttgagc gtccaggggt ccggtcagac gcgggtggag ccggacgagg cggtggtgcg
     2401 gctgggggtg accgcccagg cgccgaccgc ccgggcggcg caggaccagg tcaataagac
     2461 cgccgccgcg atcctggcgg cggtgcgcgc gctgggcgtc gccgcggagc aggtccagac
     2521 ctccgagctg tcgctgggcc cggtctactc ctccggcggt ccgcaggagc gcaacgagcc
     2581 gcggatcacc ggctatcagg cgaacaacgt ggtgtcggtg acgctggcgg agctgaaccg
     2641 cgtcgggccg gtgatcgacg ccgggctggg cgccggcgcc aaccgcctcg aaggggtgat
     2701 gttcgggctg cgcaacgacg cgccggcccg cgccgcggcc ctggccaacg ccgtcaccga
     2761 ggcccgcggc aaggccgaag cgctggcccg cgccgccggg gtccgcctgg tggagatcgt
     2821 cgagatcgtc gagggcggcg tctcggtctt cacccccacc atggcccgcg gcggccgcgt
     2881 cgccatggag gcgatggaca tggcgatggc gacgccggtc tcctccggcc aggtgtcggt
     2941 ggatgccagc gtcaccctgc gctaccggat cgccccccag gcgggcgccg gagcgccgcg
     3001 gtgacagccg ggatgaacgc cgaggaggtg gtccgcctcc tcggcctcgc cccccatccc
     3061 gaagggggct tctaccgcga gacgttccgg gatggctcgc cagggacggg ccgcggggcg
     3121 tccaccgcca tctacttcct gttgcgcgag ggggaggtct cggcctggca tcgcatcgac
     3181 gccgccgagg tctggcactg gtacgccggc gcgcccctgg agctgacgct ctcgccgggc
     3241 cgcgggcacg tcaccgtgcg gctcggcccg gatctcgcgg ccggcgagcg cccccaggcg
     3301 gtggtgccgg cgggggagtg gcagtccgcg cgcacgctcg gtgcctgggc gctggtgggc
     3361 tgcacggtcg cccccgcttt cgagttctcc acgttcgaga tggcgccgcc cggctgggct
     3421 ccggagccgg aggtgggtgc ctgatgaggt ggcgctggct tctcgccgcg ctggcgctcg
     3481 ccgcctgcga gccccgggag gtccccgggg cggccggtga gatgcctgcc gagtctccgg
     3541 aggcggtgcg gcaggcgcgg gagcgccggg agatccggca gaaatccggc tgcgtcgctc
     3601 cgtggccttc aggagcccgg gaggccgtcg agagtgtgca gaaggcggcc gccgccggca
     3661 acgccgcggc cctgcgcccc ttcatggcca gcgagctcac gtggagcttc ggcggcgatg
     3721 ccgacgcggg ccaggcgatc aagcactggc ggcagaaccc cgaggtgctc cgggagatgg
     3781 ccgcgatcct ggccaggggt tgtgtcgtgc aggagacgcc cgagggggct tccgagagga
     3841 tcgtctgccc cccggagtac gagtcccaat ccggctacct gggctaccgg gcgggcttcg
     3901 agcgccgggg ggaggtctgg aagatgacct tcttcgtcca gggcgactga cccacccccc
     3961 tacgctacgc cctcctcccg ggccgcttcg gtccagacct cgatgtcctt ggcgctcatc
     4021 tcgccgaccg gcttctcccg ggcgtcccgg tagtcccact cctccacgtc cttcaggtac
     4081 tgggagcgcg acaggacggt gccgcggcag agcccctcga cgccctggag gagcgagggg
     4141 tcgcggacct cgcccagggc gcggccgagc aggtcctcca tcacccgggc ggggatgcgg
     4201 tgcgcctccc cgggatagat gtagccgaag agcaggaggt gggtcaggag cacccgccag
     4261 tgctcggcga agcgctggag caggcggtcc cagtccaggc tctcggcgca ggagcggatc
     4321 aggtggctga tgtcgccgcc gtcgaagcgc tcgcgctcca tgatgaagga cttggaccag
     4381 atgatctcct cggccgggca gagcttcgcc ggcacgccga gcacctcctc gtccaccgcc
     4441 ttctcgaacc agaggtcgtc cacctcggcg atgccgttgc ccgaactgaa gatgaggtcg
     4501 acgaaggcgt ccccctcata ggccttgccg agccagtggg ggaacgtgcg ttccacctga
     4561 tagcccgctt tggcgaggac gtccagcgcc tggtcgaagt cgcggtggtg gacgaagatg
     4621 tcgaagtcct tggtatggcg gacgatcccg gtgtagcggg cataggcgta ggcgcccccc
     4681 accaggaacc gtacccccgc ctgctccagc ttgcgcatgg tgcgacggta gaagtcggcg
     4741 gtctcttcgc tgagatgctc tcccgagctg ctgtttccct tggctgtctg atcggtcgcg
     4801 aggctcgtgg ccatcggcgt ctcctccagt ggccatcctc tcgtgagcaa aagacgtacc
     4861 atgccgttct ggcacacctc ttgccaagat ctccggtgga cagaatggaa gggagagaga
     4921 gccttgtcac gaagagacgg aacggtacgg atcggcgccc tggccgacct ccactacacc
     4981 cggacgccgg agcagcccct gcgcgagctg ctctcccagg cgggccgcga atcggacgtc
     5041 ctcctgatct gcggcgacct gaccgaccac ggattgccgg aggaggcgca gggcttggcg
     5101 cgggagattc acggggcggt gtcgatcccg gtgatcgcgg tgctcggcaa ccacgacttc
     5161 gaggggggac agcaggagga ggtcaagacc atcctgcgcg acagcggcat ccgggtgctg
     5221 gacggcgacg ccaccgaggt ggagggggtg ggcttcgccg gcgtcaaggg gttcgccggc
     5281 ggcttcggca agcgcgccct ggggccctgg ggcgaggaca tcatcaagct gttcgtccgc
     5341 gaggcggtcg acgaggcgct gaagctggag gcggcgctcg cacgcctccg caagcggccg
     5401 cggatcgcgg tgctgcacta ctcgccgatc caggagaccg tccaggggga gccgctggag
     5461 atctatccgt tcctcggctg cagccggctg gaagagccgc tgacccgcta cccggtggac
     5521 gcggtcttcc atggccacgc ccaccgcggc tgtcacgaag gcaagaccgc ctcggggatc
     5581 ccggtcttca acgtcagcat ccagcttctg cgccagacct atcccgaccg gcccgcgttc
     5641 atgaccttcg aggtgaatgt ggacgaggag gccgacccat ctcagcaacg gcagcccggc
     5701 gaggaccgcc gggtccagga acgacgaaag gagtgacacc atgccgaccg cctggagcga
     5761 caaggacgag cgccagtacg agcacatcaa ggagagctcc gaggagcgtg gcgtgccgga
     5821 gaagcgcgcc aaggagatcg ccgcccgcac ggtcaacaag aaccggcgcg aggaggggcg
     5881 cacccccaac aagaccaccc aggggacggg caaccccaac aaggggctcg aggcgcgcaa
     5941 ggtcgacgag ctgcgcaaca tcgccagcga cctcgagatc ccggggcgga gcaagatgaa
     6001 caaggcggat ctggtgaagg cgatccgcgg caagcggtcc cactgaggcg gcccggacgc
     6061 tgtagtagac ttgccgggcg cgccgcgggg acagcgtccg atggagagca gcgaagggtt
     6121 cgttttcatc cggccgggcc tcaacctgta ctaccagtcg gtggggcagg ggccggaggc
     6181 ggtcgtcatc cccaacgcca actggctggg gcacgcgctc cagccgctgg ccgaggggcg
     6241 gcggctgatc ttctacgacc cgcgcagccg cgggcgctcc agcacggtga ccgacccgcg
     6301 ccagctcggc attgaggagg acgtcaacga cctcgaggcg gtgcggcgct tcttcggcct
     6361 ggagcgcatg gccctcctcg gctcctccta tcacgcggcg atcaccgccc tctacgccat
     6421 ggagtacccg gagaaggtgg cgcgcctgct cctggtctgc ccgatcacgc cgcgccgccc
     6481 gggcgagtgg gcgcaggaga cgccgaccgc cgacatgctg gtctacccgc ccggcatccc
     6541 gcggctggag gagatgcggc gcgacgcggt cgaccgcgag gacccggtgg ccttctgccg
     6601 cgcctggttc acccacttcc tgctgccggc ccagatgggg gagccgtccg ccatcctccg
     6661 cttcccggtg gaggacgtct gctccttccc caacgagtgg ccgagccaca ccatgagcct
     6721 ctacttcgag cacatcatcc cgaagatgga ggactgggac ttcaggccgc gcttggcggg
     6781 tctcgagacc ccgctcctgg tgatccaggg gacggacgac ctggtgcctc tcgaggcctc
     6841 ccgcgactgg gccgcctacg ctccgaacgc catgttcctt cccctcgccg gggcggggca
     6901 ccacccctat ctggagcgtc cccgcgagtt cttcagcgcc gccgaccgct tcttcaaggg
     6961 ggagtggccg cagggggcgc aggccgtcca gcacctcgcc tgacgagagc cggcacggcc
     7021 gcggcgaggg cggcccccag gaacagcgcg ttcgtctctc ccaccagccg gacgaggagc
     7081 cccaacgaca gggaaccggc ggccgggtat ccggccgagc cggcgaggac cagggccgcg
     7141 gccaccgcct ggcccgccag gaccgcggcc agcccggcgg cgaaggcgcc ggcctggggg
     7201 gagcgggcga aggctgcagc gagcccgaag accacagcga gcgccggcag ggcttggcgg
     7261 agcgccgccg ccgccaccgc tccgggcagg cccagcgggc cgtccgcgag ggcgaacgcg
     7321 agccggacgt ccagcgtcga gaggctcgcc gtctctccca ggccgcgggc gaggaccgcg
     7381 ggcgcgatgc cgcagagcat gccgcagagg agaggggcac gggggaggag ccgcctcccc
     7441 aggagcgcgc ccagccccag gcccgcgagg acggccagga tcaggacggc cgggccgccg
     7501 gcggacagat ctccggacgt ggccgaagcc caggcttctc cgaggcggag cagcccgaaa
     7561 aggccgccgg cggcgaaggc cgcccagggg agagatcccc ggcgcccgga ccggactgcc
     7621 atcgccaccc agcccaggct cgcgagggag gcggcgaggg ctagccggtg gagccccagc
     7681 gccgccgcgg cgagtgacag ccacagggtc gcgttgagga ggaagggggc gcgcctatgg
     7741 atctcgccct gggcgaccgc acgccagagg aggaggcagg ccccggcggc caggagggcg
     7801 aggagcggca ccggccatcc ggtgccccgc tcagccggct ggacgggagg cgccgggagg
     7861 cccagggggg cgaggacgag cgcggcgacg ccgctctggc ggacggcgcc gaaccggccg
     7921 cgcggcagcc cgggcccggc cacgaccagc ggtgtccgca cgaccgcggg ctccgggccg
     7981 gcgtgcccgc cgctgccggt gaccccgtga tcggaggtga ccaccaccgc ggtgccggca
     8041 ccggccaggg agaggaggcg gccgagcgcc gcgtcggcgc gcgtcagggc ctgccggtgc
     8101 tccggcgagc gggcgccatg gaggtgggcc gcgtcgtccg gctcgctgag gtggacgacg
     8161 accaggccgt accggccccc ggcgagggct gcggcgccgg cggcgagcac ccggtggtcc
     8221 tcgccggaga ccgtgcggac cgccagggaa ccgtcgagcc aggagccgta gagatcggcc
     8281 cagaggcgcg gcccggcggc gaaggtccgg agccccgcgg cccgggcggc ggcgaagatc
     8341 ccgccggatg gcgccggaga ggcgccgaag tcctgaagga aggaggccgg aggcgggacc
     8401 tccccttcgg ccagcgtccg gatgcccgcg atggtcgagg ggatcagcga ctcgaccagg
     8461 gcggtcccgg tgctgccgcg atgggccagc ccggccaact ggggcatcag ggttgcgtcg
     8521 aaggcgtggt cggcgcggag cccgtcgatc accacgagca ggacccgctg agcccgggcc
     8581 gggccggagg ggggagaagg agggggctgg aagccggccc ggtcgccgga aagccgcggg
     8641 cccagggcga gcgccaggat cagcaggagg agggcgacgg agttgccggc ggcggcaagg
     8701 gcgtgcagcc ggggtctcag cgcgtctcca acgtctccgg cgccgggctc ggcccgtacg
     8761 accgtccctt ccacgcggca gggcggccca ggaggtggcg ggtgagagcg gtccattgga
     8821 tggcgaggaa gagcaggatg ctcaccgggt ggagcagcgc gccgtccagc ggctgccgga
     8881 accggcgcgc cgcggccaga cggggcaggt agacggccag cgttcccgcc agagaggccc
     8941 cgagcgccac cgcgtcaccc ttgatcaaag ccggcaggag cagcagcggc ggcagcactt
     9001 gaccggtgag gagcagggcc gtcaccggca ggatcttgcc cggcgaggcc accccctcga
     9061 cggcgttctt ggccaggccc cgccagacct cccccgcgtt cgtgtacatg cggcacgagg
     9121 cggcgtccgt ggcatcgaag agatcggtgg cgaagccggc gcggcggaag gcgcggggga
     9181 gggcgatgcc atcgtggagc gaggcggcga tggcgctgtg gccgccggcg cgctcatagg
     9241 cgtcgcgccg ggcgaagaag agctgaccgc agccggcgcc gtaggccggg tgggtgctgc
     9301 gccgcatgcg ggccatgggg aggaagccca ggagcacgaa gtgcatcagg gggatcagca
     9361 tccgttcgag gagcgtctcc gtctcctggc gcgggaagcc gctcaccagc ccggcgccgc
     9421 tcgccttcag gaaggcggtg ccgcgggcga ggccgcccgg ggtgaggcgc acgtcggcgt
     9481 ccgcg
//
LOCUS       DASPJI010000012        11569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_515630, whole genome shotgun sequence.
ACCESSION   DASPJI010000012 DASPJI010000000
VERSION     DASPJI010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11569)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11569
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515630"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VEL74_00330"
     CDS             <1..445
                     /locus_tag="VEL74_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HYG60999.1"
                     /translation="WLARLREYLEGTLRKTKPLLLLGDFNIAPDERDVHDPDLWRGKI
                     HFHPSEHRALGRIMGLGLHDLFRKHHEEGGFYSWWDYRALAFPKNNGLRIDLMLGNEP
                     LLERCTSCVMHRDERKGETPSDHIPVVAEISEAKPPARKKAAGKA"
     gene            complement(442..1092)
                     /locus_tag="VEL74_00335"
     CDS             complement(442..1092)
                     /locus_tag="VEL74_00335"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYG61000.1"
                     /translation="MSHSPHSLRLFLIRHGETVSNREMRYLGSRDEPLAESGLLQAGR
                     LAEALAPLPVRAVYASPLRRAADTASRVAAARDLPLLLEPRLREQCFGEWEGLTRTEI
                     LAGQEREELLRWEADLSLPPPGGESLAAVQERVLELAEDLGRRHAGDWIALVSHVGPI
                     KALLCAALGVPLAAARRMFLDPGTLCVVDWGERPVVRLFNSHGHLGWESARWMSAG"
     gene            complement(1150..1395)
                     /locus_tag="VEL74_00340"
     CDS             complement(1150..1395)
                     /locus_tag="VEL74_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61001.1"
                     /translation="MNERSSSEWSEPVMAIGCIAAVLWGIGVSLRAFFLGPLAIDWTM
                     WAVQLVAGIIVCLLAARMGLWAADRYARRSRVASVQP"
     gene            complement(1498..2475)
                     /locus_tag="VEL74_00345"
     CDS             complement(1498..2475)
                     /locus_tag="VEL74_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61002.1"
                     /translation="MGLLSGTVSVTRFNAAVPAEGPDFERAQFWAIEPGSEVRERMGF
                     VPFEIEAPWEVAKGRYVFRVRIDKVRPDPTAVKERLRELIKAEMDATGKTFVGSKTRK
                     KLKELAEAESCMQQAPRTKIIECCLDNNLLYVASTAKTYLGTVLTLLRQTGVEADFKA
                     PWLDEAPEVDEASDVVLPKEPGQSVLGCRFVRALLDEPDVMVEPETGSVRLATRETKV
                     SLTGAILHDLHRYLEEGAEILSAKLHVGPVQMRFDALSYRLNGVKLEPVKNEHWTIDL
                     DQRLEQIAAVFEALDTKYAALKGRLGEEYVAPGSMPPDAQPVEAAAAGA"
     gene            complement(2495..3829)
                     /locus_tag="VEL74_00350"
     CDS             complement(2495..3829)
                     /locus_tag="VEL74_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61003.1"
                     /translation="MRARIASALILAVLVAVCPRPGTSAPARPAPAPPPAWQLLQKSE
                     RSVVHVADAARLSPAGLRAGSPLYAPFWDALEEMDRALAEVRSSLGVRNQRFFSALRG
                     GSRSLAQLRVVWSRTGIHEPAVTRALESLGSSYRPLRTHYGPEQARAEQGGALTPQER
                     DRFREIQRMQVRLIAMLQPLRTQAAGRGDREMTEHVDRLLERANDIASADLTLDEYLE
                     ASLSEDELMGEWDGNGPYVEPSYRSAWHAADAAVEELFVAEEASFVFALDLGGPGAKG
                     AGGDLAGVGEPAAPATEQPLGGQPATDQPTVTVYGGAEGEELFDDPLFGDAAEEADPV
                     EVIEEPEITEAGAFEGVEEEASPAEQVISEGAEEAENETAAEEVVEEGKPEEAAPISE
                     EDIPAEDLPVQTGVEDSKATPAAPPAKVPESNAEGERKPPLGVATLAAILPL"
     gene            complement(4045..4647)
                     /locus_tag="VEL74_00355"
     CDS             complement(4045..4647)
                     /locus_tag="VEL74_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYG61004.1"
                     /translation="MNTPDHDTYREWLHLEADGELSPAQHTRLEAHLATCADCRRQLQ
                     EYRALDGLIARGSLPVREDFKSGVMASLPVVGWEARHPRTWRFPAAVFALFAGLAAAL
                     VSFGSEGPAPSGLSALFAVAGLLRAAVQAGAGLLAASWKGLGMAFGDVMSSPLSLGTA
                     AFLVLCLNLLLISLIRRRKPAASEAAVPANRKGGNGKNAG"
     gene            complement(4662..5246)
                     /locus_tag="VEL74_00360"
     CDS             complement(4662..5246)
                     /locus_tag="VEL74_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG61005.1"
                     /translation="MNRGAEQPADEDLVAAILAGDAERFGDLVDRYQGRLVNYLFRLL
                     RNADDAHDLAQEVLVKVYQALDRYDPQYRFSTWLFRVAQNAAIDQIRRRRLKLVSLRQ
                     EVDEGEERDWDLPSPDRGPYGEMRNRERGDAIQQAIDALPWEYRELILLRHFGELPYE
                     EIARLKQMPLGTVKNKLFRGRQMLKDKLQDFLSD"
     gene            complement(5332..5967)
                     /locus_tag="VEL74_00365"
     CDS             complement(5332..5967)
                     /locus_tag="VEL74_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HYG61006.1"
                     /translation="MNAIAPGKIWLAAVLCVATACSADTRQTTSATAATESPAAAEAP
                     APAAPTPVTPVGPVTREQVESAVPDWVQAQAESQIDAAAAQGLAAVEPGAEVTIFLGT
                     WCGDSRRELARLWRALDETGGTVPFDIRYIAVDEAKAQPADRIAESAIRFVPTLIVSR
                     GGREVGRIVEHSPNGVEVDLLALLTGKAQGVVTASPNAAENPAEGAASPSH"
     gene            complement(5982..8042)
                     /locus_tag="VEL74_00370"
     CDS             complement(5982..8042)
                     /locus_tag="VEL74_00370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYG61007.1"
                     /translation="MRAARRLVLSLSLVLLLGACATAGSGGPQGPSDLPSIEVRDLDQ
                     RALLLLLVDRQIYEPVAVQEALRGGPELREELAAALGRIPDRQGMRVLAGLLLDDAPA
                     VRRAAAFGLGELEDPDAIPILLPALRDADREVGVLAAEALGKLGAPVTEVVEVLLPLS
                     EEERWARLLPHLFRFKEEATVTLARRGLERPEPELHARAAYALARDPRPEAAPDLRRL
                     VADPDPQVRAWAARGLGIVGGAEDLAALRPLLEDAQPGPVILALRAARALITRETASA
                     PVPEDWRPRLAGLLADPRPGVRVTAAEAAGAWPMADGGGVLGEALAAKAAGEDGRERG
                     LALVSLAAAGHPRAGELAAAAAGSADETVRARAAEAAVLLKREELTGRLAADPSPLVR
                     TAALDARLAAVPEGEGAGLARQALADADASVRASALGWLAEHPVLPLADLDRAAAAAL
                     RDPVDDPAVAAVEALAARAKANDTQPGEKGGLVALLQKMAGHPSAVIRKAAGEGLTEL
                     GSPVPPPPPTAATRSAETSRSLDGYREIVQRTRRPREVEVRTEKGAFTIRLACPDAPL
                     TCLNFLQLAGQGFYDGLRFHRVVPDFVIQGGDPRGDGSGGPGYAIRDEINRLRYRRGA
                     VGMALSGPDTGGSQLFVTLSPQPHLDGGYTLFGYVTAGEEVLDRILQGDRIESVRELG
                     DAAR"
     gene            8086..8703
                     /locus_tag="VEL74_00375"
     CDS             8086..8703
                     /locus_tag="VEL74_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYG61008.1"
                     /translation="MDAASLRFADVTRRFGRLRVLRGVSADVGSGEALLVTGHNGSGK
                     STLLRCLAGLLAPDAGTIEYHEGGRALEVAERRRRVGLVAPDLAFYGELTVAENLEFF
                     ARLRRVDVRRGLDLLGRLGLPSDQLVGALSSGMRQRLRWAFALLHRPRLLLLDEPFQN
                     LDAAGESATRALLAEHLNRAGGLAVIANPFDLELDRVAGRLHLGG"
     gene            8672..9358
                     /locus_tag="VEL74_00380"
     CDS             8672..9358
                     /locus_tag="VEL74_00380"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HYG61009.1"
                     /translation="MWRDASTWAAEAAAVFAKDWRCEFRTRYALNTLALFAFTTLVVV
                     SVSLGPLGVSAAKGTPVLPTLLWLILLFAAAAGLPRSFVHEEETQTATALRLAATPSA
                     LFCGKLLYCLTLVAALEVLITPVYLAMMSLEVHSPWLLVGTLAAGGYGLAAGSTLIAA
                     MIAQARGKGTLFAVLAFPVLLPLLLLAVELTRHAVSGEPAGMALTQILLYDGTVTVAA
                     LMLFPVVWNP"
     gene            9360..10031
                     /gene="ccsA"
                     /locus_tag="VEL74_00385"
     CDS             9360..10031
                     /gene="ccsA"
                     /locus_tag="VEL74_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HYG61010.1"
                     /translation="MKTILAWLLWLWMCLVLYGAFLYARPAMGFAAGQSSRIVFFHVP
                     MAWVSMIAFLAAAVWSARYLLGGRRAEHDRAAAVAIQLGLLFGVLATVTGAMWARIEW
                     GAFWNWDPRQTSITLSLLFYAAYLALRSAVEDAETRGRLSAVYALLGVVITPFLYFIV
                     PRITFSLHPEPLVNADGKIDMDSEMRQVLFASFLGFTALFFWMHNLQCRLQALADRKS
                     AHFYE"
     gene            10048..10164
                     /locus_tag="VEL74_00390"
     CDS             10048..10164
                     /locus_tag="VEL74_00390"
                     /inference="COORDINATES: protein motif:HMM:NF040113.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HYG61011.1"
                     /translation="MGNGGIEWVVAVNLVIWTGLFIYLLRLGKKLRKLEKEP"
     gene            10161..10556
                     /locus_tag="VEL74_00395"
     CDS             10161..10556
                     /locus_tag="VEL74_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HYG61012.1"
                     /translation="MKRNRVYLFGGFLLLAFAGFSFSAFKETLTPYVSYEEARRGGQV
                     QVAGALVKGSSSYETAKDAFYFTIHDPEDANETIRVRYHGLKPANFEEAISIVAIGSY
                     QAGANEFTADKLLVKCPSKYQGAEVKEYT"
     gene            10560..>11569
                     /gene="ccsA"
                     /locus_tag="VEL74_00400"
     CDS             10560..>11569
                     /gene="ccsA"
                     /locus_tag="VEL74_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HYG61013.1"
                     /translation="MDYVSQLYLPGALTLWCATLFALSSLWGYALVLRGDAGSLTFAR
                     RAYGFFTLSIVAASALLGLLLLMRDFRIEYVYQYSGLDLPAHYQFAAFWAGQKGSFLI
                     WLFWGALIGLLVRRTTREDEPAVMGIYTLTLLGLLFILVRENPFVMLRETPVDGQGLN
                     PLLQDDWMTIHPPIMFVGYAATAIPFSFAMASLWRRDYTGGWARRAFPWALGGFLVLG
                     AAILMGGYWAYKTLGWGGYWGWDPVENASLIPWILGTTLIHGLYMERSKGRYRRVNYI
                     LAALTFLSVLYGTFLTRSGVLADFSVHSFVDLGISGWLIAVMATFIGMSIYLLASRLR
                     EVP"
BASE COUNT         1562 a         4241 c         4083 g         1683 t
ORIGIN      
        1 gtggctcgcc cggctgcgcg agtacctgga ggggaccctg cgcaagacca agcccctgct
       61 gctcctcggc gacttcaaca tcgcgcccga cgagcgcgac gtgcacgacc ccgacctctg
      121 gcgcggcaag atccacttcc atcccagcga gcaccgggcc ctcgggcgca tcatgggcct
      181 ggggctgcac gacctcttcc gcaagcatca cgaggagggg ggcttctact cctggtggga
      241 ttaccgcgcg ctggccttcc cgaagaacaa cggcctgcgg atcgacctca tgctgggcaa
      301 cgagccgctg ctcgagcgct gcacctcctg cgtcatgcac cgcgacgagc gcaaggggga
      361 gaccccctcg gaccacatcc cggtggtggc ggagatcagc gaggccaaac ccccggcgcg
      421 gaagaaggcg gccgggaagg cctagccggc gctcatccag cgcgccgact cccagccgag
      481 gtggccgtgg gagttgaaca ggcggacgac cgggcgctcc ccccagtcga cgacgcagag
      541 ggtgccggga tcgaggaaca tgcggcgggc ggcggccagc ggcacgccga gggcggcgca
      601 gagcagggct ttgatcgggc cgacgtggct caccagggcg atccagtcgc cggcgtgccg
      661 ccggcccagg tcctcggcga gctccagcac ccgctcctgg acggcggcga gcgactcgcc
      721 gcccggcggc ggcaggctga ggtccgcctc ccagcgcagc agctcctccc gttcctggcc
      781 ggcgagaatc tcggtccggg tcagcccctc ccactcgccg aagcactgct cgcgcagccg
      841 cggctcgagg agcaggggga ggtcccgcgc cgcggcaacg cggctcgcgg tgtcggcggc
      901 ccgccgcagg gggctggcgt agacggcgcg gaccggcagg ggggccagcg cctcggccag
      961 acgcccggcc tggaggaggc ccgactcggc gagcggctcg tcccggctgc ccaggtagcg
     1021 catctcccgg ttggagacgg tctcgccgtg gcggatcagg aacaggcgca gggaatgggg
     1081 ggagtggctc atggtcgcgg cgacgcgcca gagcctatcg cgaatcggtg agcaagggag
     1141 cgcccgggct cagggctgga cgctcgccac tcgggacctg cgcgcgtagc ggtccgccgc
     1201 ccacaggccc atgcgggcgg cgagcaggca gacgatgatg ccggcgacga gttggacggc
     1261 ccacatggtc cagtcgatgg cgagcggccc gaggaagaag gcgcgcaggc tgacgccgat
     1321 cccccagagc accgcggcga tacagccgat cgccatgacc ggctcggacc actcggagct
     1381 gcttctctcg ttcactggca taccctcccg gagacctgtc gtcttgtctt ggggtgcgtg
     1441 gggaaggtcc cacgccaccg tctccgggaa gacaggacga gcaaggggcg tgccgggcta
     1501 ggctccggcc gccgcggcct cgaccggctg cgcgtccggg ggcatggagc ccggcgccac
     1561 gtactcctcg cccaggcgcc ccttgagggc ggcgtacttg gtgtccaggg cctcgaagac
     1621 ggcggcgatc tgctccaggc gctggtcgag gtcgatggtc cagtgctcgt tcttcaccgg
     1681 ctcgagcttg acgccgttca gccggtagga gagggcgtcg aagcgcatct gcaccgggcc
     1741 gacgtggagc ttggccgaga ggatctcggc cccctcctcc aggtagcggt ggagatcgtg
     1801 gaggatggcg ccggtgaggc tgaccttggt ctcgcgggtg gcgagtcgga cgctcccggt
     1861 ttcgggctcg accatgacat cgggctcgtc gaggagggcg cgcacgaagc ggcagccgag
     1921 gaccgactgc cccggctcct tggggagcac cacgtcgctc gcctcgtcga cctccggggc
     1981 ctcatccagc cagggggcct tgaagtcggc ctccacgccg gtctggcgca gcagggtgag
     2041 cacggtcccc aggtaggtct tggcggtgct cgccacatag aggaggttgt tgtccaggca
     2101 gcactcgatg atcttggtgc ggggcgcctg ctgcatgcag gactcggcct cggccagctc
     2161 cttgagcttc ttgcgcgtct tggagccgac gaaggtcttg ccggtggcgt ccatctcggc
     2221 cttgatcagc tcccgcagcc gctccttgac cgcggtcggg tcgggccgga ccttgtcgat
     2281 ccgcacccgg aagacgtagc ggcccttggc cacctcccag ggggcctcga tctcgaaggg
     2341 gacgaacccc atccgctcgc gcacctcgga cccgggctcg atggcccaga actgcgcgcg
     2401 ctcgaaatcc ggcccctcgg cgggcaccgc tgcattgaaa cgggtgacgc tgaccgtccc
     2461 gctcagaaga cccatgtcgt acctcctgga agattcaaag gggaagaata gccgccagag
     2521 tcgccacgcc caagggcggc ttgcgctccc cctcggcgtt gctctcgggg accttggccg
     2581 gcggggcggc cggagtggcc ttgctgtcct cgacaccggt ctggaccggg aggtcctccg
     2641 cggggatgtc ctcctccgag atcggggcgg cctcctccgg cttcccttcc tccacgacct
     2701 cctcggcggc agtctcgttc tcggcctcct cggccccctc ggagataacc tgctcggccg
     2761 gcgaggcctc ctcctccacc ccttcgaacg cccccgcctc ggtgatctcc ggctcctcga
     2821 tgacctcgac cggatcggcc tcctcggcgg cgtcgccgaa cagcgggtcg tcgaacagct
     2881 cctcgccctc ggccccgccg tagaccgtga ccgttggctg gtccgtggcc ggttgcccgc
     2941 cgagcggctg ctccgtggca ggagcggcag gctcccccac gccggcgagg tccccgccgg
     3001 cccccttcgc ccctggcccc cccaggtcga gcgcgaagac gaagctcgcc tcctcggcga
     3061 cgaacagctc ttcgacggcc gcgtccgccg cgtgccaggc gctccggtag gagggctcga
     3121 cataagggcc gttgccgtcc cactccccca tcagctcatc ctcggagagg gaggcctcga
     3181 ggtactcgtc gagcgtgagg tcggcgctgg cgatgtcgtt ggcccgctcc agcaggcggt
     3241 cgacatgctc cgtcatctcc cggtctccgc ggccggccgc ctgcgtgcgg agcggctgga
     3301 gcatggcgat cagccgcacc tgcatgcgct ggatctcgcg gaagcggtcc cgctcctggg
     3361 gagtgagcgc tcccccctgc tcggcccgcg cctgctccgg cccgtagtgg gtgcggagcg
     3421 gccggtagga ggagccgagg gactccagcg cccgcgtcac cgccggctcg tggatgccgg
     3481 tgcgcgacca gaccacccgg agctgggcca gcgaccggct gccgccgcgg agggcgctga
     3541 agaaccgctg gttccggacg ccgagggagc tccgcacctc ggcgagggcg cggtccatct
     3601 cctccagggc gtcccagaag ggggcataca gggggctgcc cgcccgcagc ccggcgggtg
     3661 acaggcgcgc cgcgtccgcc acgtggacca cggaccgctc cgacttctgg aggagctgcc
     3721 aggcgggggg aggcgccggg gccgggcggg cgggcgcgga cgtgccggga cgggggcaca
     3781 ccgccaccag gacggcgagg atcagggcag aggcgatacg ggctctcatc gaaggactcc
     3841 gtcggcacgt cgggggtgca acaggcttct acttatatat acggacgagg acggcggagc
     3901 gtcaacagaa gcaccgatgc tgcggactac aaaacgagaa cggtaccggc ggcggaaacg
     3961 ccgataccgt cctcaacccc agaaaaggag aaagaccatg cgcggtatag tacagagtcg
     4021 ggaccgcgca gggaagtccg caactcagcc ggcgttcttg ccgttcccac ctttccggtt
     4081 ggcggggacg gcggcttccg aggcggcggg cttgcgccgg cggatgagcg agatcaggag
     4141 caggttgagg cagaggacca ggaaggcggc ggtcccgagg ctcagcggcg aggacatgac
     4201 gtcgccgaag gccatgccca gccccttcca ggaggcggcg agcaggccgg cgccggcctg
     4261 gaccgcggca cggagcagcc ccgccacggc gaagagggcc gacaggcccg acggcgccgg
     4321 accctcggag ccgaagctca ccagcgccgc ggccagaccg gcgaacagcg cgaagaccgc
     4381 cgccgggaag cgccaggtgc gcggatggcg cgcctcccag cccaccaccg gcagggacgc
     4441 catcacgccg gacttgaagt cctcccgcac cggcaggctg ccgcgggcga tcagaccgtc
     4501 gagggcccgg tactcctgga gctgccgccg gcagtccgca caggtggcga gatgggcctc
     4561 gagacgggtg tgctgagcgg gcgagagctc gccgtcggcc tccaggtgca gccattcgcg
     4621 gtaggtatcg tggtccggcg tgttcatcgt gtccctcgcc ctcagtcgct gaggaagtcc
     4681 tggagcttgt ccttgagcat ctgcctgccg cggaagagct tgttcttgac cgtcccgagc
     4741 ggcatctgct tgagccgtgc gatctcctcg tagggcagct cgccgaaatg gcgcagcagg
     4801 atcagctccc ggtactccca gggcaacgcg tcgatcgcct gctggatggc atccccacgc
     4861 tcccggttgc gcatctcgcc atagggcccg cggtccggag acggcaggtc ccagtcccgc
     4921 tcctccccct cgtccacctc ctggcgcagc gacaccagct tcagccgccg gcggcggatc
     4981 tggtcgatcg ccgcgttctg ggccacccgg aacagccagg tcgagaacct gtactgcggg
     5041 tcgtagcggt ccagcgcctg gtagaccttg accaggacct cctgcgccag gtcgtgggcg
     5101 tcgtccgcgt tgcgaagcag ccggaacagg taattgacca ggcggccctg gtagcggtcg
     5161 acgagatcgc cgaagcgctc cgcgtcaccc gcgaggatcg ccgcaaccag atcctcgtcg
     5221 gccggctgct ccgctccccg gttcatgggt tagtacgcaa ggcgccggcc aaggttgcag
     5281 cggctcgcga agaggagggc ggcagcccgg cgagcatgcg aggaccccgg ctcagtggga
     5341 gggagaggcc gcgccttcgg ccgggttctc ggccgcgttg gggctggcgg tgaccacgcc
     5401 ctgggccttg ccggtgagga gcgccaggag atcgacctcg acgccgttgg gagagtgctc
     5461 gacgatccgc ccgacctcgc ggccgccgcg gctcacgatc agggtcggca cgaagcggat
     5521 ggcgctctcg gcgatccggt ccgccggctg cgccttcgcc tcgtccaccg cgatgtagcg
     5581 gatgtcgaag ggcacggtgc ccccggtctc gtcgagcgcc cgccagagac gggccagctc
     5641 gcgccggctg tcgccgcacc aggtccccag gaagatggtg acctcggcgc cgggctccac
     5701 cgccgccagc ccctgggccg ccgcggcgtc gatctgcgac tcggcctgcg cctgcaccca
     5761 gtcgggaacc gcggactcca cctgctcccg ggtcaccggg ccgaccggag tcaccggagt
     5821 cggcgcggcc ggagcgggag cctcggcggc ggccggagat tcagtggctg ccgtcgcgga
     5881 cgtcgtctgc cgggtgtcgg cgctgcaggc ggtggccacg caaaggacgg cggccagcca
     5941 gatctttcca ggggcgatgg cgttcatgac cgcggagtct atcaccgcgc cgcgtccccc
     6001 agctcgcgca cgctctcgat gcggtccccc tggaggatcc ggtccagcac ctcctcgccc
     6061 gcggtcacgt acccgaacag ggtgtagccg ccgtccaggt ggggctgcgg cgacagggtg
     6121 acgaagagct ggctcccgcc ggtatccggg ccggacaggg ccatgcccac cgccccccgc
     6181 cggtagcgca ggcggttgat ctcgtcgcgg atggcgtagc ccgggccgcc gctgccgtcc
     6241 ccccgcgggt cgccgccctg gatcacgaag tccggcacca cgcggtggaa gcgcaggccg
     6301 tcgtagaagc cctggccggc gagctgcagg aagttgaggc aggtgagcgg cgcgtccggg
     6361 caggcgagcc ggatcgtgaa ggcccccttc tccgtccgca cctcgacctc gcggggccgg
     6421 cgggtgcgct gcacgatctc gcggtagccg tccaggctgc gggaggtctc ggcggaccgc
     6481 gtcgcggcgg tcggcggggg cggcggcacc gggctcccga gctcggtcag cccctccccg
     6541 gccgccttcc ggatgaccgc ggaggggtgc cccgccatct tctgaaggag ggccacgagg
     6601 ccccccttct ccccaggctg ggtgtcgttg gccttggccc tggccgccag cgcctcgacg
     6661 gcggcgacgg ccggatcgtc caccgggtcg cgcagggcgg cggccgcggc ccggtcgagg
     6721 tcggccaggg ggagcaccgg atgctcggcg agccagccca gggccgaggc ccggaccgac
     6781 gcgtccgcgt cggccagcgc ctgccgcgcc aggccggcgc cctccccctc ggggaccgcc
     6841 gcgagacggg cgtcgagcgc cgccgtccgc accagggggg agggatcggc ggcgaggcgg
     6901 ccggtcagct cctccctctt caggagcacc gccgcctccg ccgcgcgcgc ccgcacggtc
     6961 tcgtcggcag agccggccgc ggccgccgcc agctcgcccg cccgcgggtg cccggcggcc
     7021 gccagggaga ccagcgccaa gccgcgctcg cggccgtcct ctcccgccgc cttcgccgcc
     7081 agcgcctcgc ccagcacgcc gccgccgtcc gccatcggcc aggcgcccgc ggcctcggcg
     7141 gccgtgacgc gcacgccggg acgggggtcg gccagcagcc cggcgaggcg ggggcgccag
     7201 tcctccggca ccggcgcgct ggccgtctcg cgggtgatca gcgcgcgggc ggcgcgcagg
     7261 gcgaggatga ccgggccggg ctgggcgtcc tcgaggagcg gccggagcgc cgccagatcc
     7321 tcggcgccgc cgacgatccc caggccgcgg gcggcccagg cgcgcacctg cgggtcggga
     7381 tcggccacca ggcggcgcag gtcgggggcc gcctcgggcc gcgggtcgcg cgccagggca
     7441 taggcggcgc gggcgtggag ctccggctcg ggccgctcca ggccgcgccg ggcgagggtg
     7501 accgtcgcct cctccttgaa ccggaacagg tgcggcagca gccgcgccca gcgctcctct
     7561 tcagataaag gaaggagaac ctccaccacc tcggtcaccg gggcccccag cttgccgagc
     7621 gcctcggccg ccagcacccc cacctcgcgg tcggcgtcgc gcagcgccgg caggaggatc
     7681 gggatcgcgt ccggatcctc cagctccccc agcccgaagg ccgcggcccg gcggaccgcc
     7741 ggcgcgtcgt cgaggagcag cccggccagg accctcatcc cctggcggtc ggggatccgc
     7801 ccgagggcgg ccgccagctc ctcccgcagc tcgggcccgc cccgcagcgc ctcctgcacc
     7861 gccaccggct cgtagatctg ccggtccacc agcaggagga gcagggcccg ctggtcgagg
     7921 tcccgcacct cgatgctggg caggtcggag ggtccctggg gcccgccgct cccggcagtg
     7981 gcgcaggccc ccagcaggag gacgagggac agggagagga cgagtcggcg ggcggcgcgc
     8041 ataggccacc gatgctatca ggggcatcga agatatattg cagccgtgga cgccgcctcc
     8101 ctccgcttcg ccgacgtgac ccgccgtttc ggacggctca gggtcctgcg cggcgtctcg
     8161 gccgatgtcg ggagcggcga ggcgttgctg gtgaccggcc acaacggctc gggcaagagc
     8221 accctgctgc gctgcctcgc gggtctcctg gcgccggatg ccggcaccat cgagtaccac
     8281 gaggggggca gggccctgga ggtggccgag cggcgccggc gggtgggcct tgtcgctccc
     8341 gacctcgcct tctacggcga gctcaccgtg gccgagaacc tggagttctt cgcccgcctg
     8401 cgccgcgtcg acgtccgccg gggcctcgat ctgctcggcc gcctcggcct gccgtccgac
     8461 cagctggtgg gggcgctctc gtccggcatg cggcagcgcc tgcggtgggc gttcgccctc
     8521 ctccaccggc cgcggctgct gctcctcgac gagccgttcc agaacctcga cgccgccggc
     8581 gagtcggcga cgcgggcgct cctcgccgag cacctgaaca gagccggcgg cctcgccgtc
     8641 atcgccaacc ccttcgacct ggagctcgac cgtgtggcgg gacgcctcca cctgggcggc
     8701 tgaggcggcg gccgtgttcg ccaaggactg gcggtgcgag ttccgcaccc gctatgccct
     8761 gaacacgctc gccctcttcg ccttcaccac cctggtggtg gtgagcgtca gcctcggccc
     8821 gctcggcgtg tcggcggcca aggggacgcc ggtgctgccg accctcctct ggctgatcct
     8881 gctcttcgcc gccgccgccg gcctcccccg ctccttcgtg cacgaggagg agacgcagac
     8941 cgccaccgcc ctgcgcctgg cggccacgcc gtcggccctg ttctgcggca agctgctgta
     9001 ctgcctcacc ctggtggccg cgctggaggt gctgatcacc ccggtctacc tggcgatgat
     9061 gagcctcgag gtccactcgc catggctgct ggtgggcacc ctcgccgccg gcggctacgg
     9121 cctcgcggcc ggcagcacgc tgatcgcggc gatgatcgcc caggcccggg gcaaggggac
     9181 gctgttcgcg gtcctcgcct tcccggtgct cctccccctc ctgctgctgg cggtggagct
     9241 gacccgccac gccgtctccg gcgagccggc gggcatggcc ctgacccaga tccttctata
     9301 tgatgggacg gtcaccgtag cggcgctcat gctcttcccc gtggtatgga atccctgaga
     9361 tgaaaacgat cctcgcctgg ctgctctggc tctggatgtg cctggtcctc tatggcgcct
     9421 tcctctacgc gcgcccggcc atgggcttcg ccgccggcca gtcgagccgc atcgtcttct
     9481 tccacgtgcc gatggcctgg gtgtcgatga tcgccttcct ggccgcggcg gtgtggagcg
     9541 cccgctacct cctcggcggc cggcgggcgg agcacgaccg cgctgcggcg gtggcgatcc
     9601 agctcggcct gctcttcggc gtcctcgcca ccgtcaccgg cgccatgtgg gcgcggatcg
     9661 agtggggcgc cttctggaac tgggacccgc gccagacctc catcaccctg tcgctgctct
     9721 tctacgccgc ctacctggcg ctgcgctcgg cggtcgagga cgcggagacg cgcggccggc
     9781 tgtcggcggt ctatgccctg ctcggcgtgg tgatcacccc cttcctctac ttcatcgtgc
     9841 cgcggatcac cttctccctc cacccggagc cgctggtcaa cgccgacggc aagatcgaca
     9901 tggactccga gatgcggcag gtcctcttcg ccagcttcct ggggttcacc gcgctgttct
     9961 tctggatgca caatctccaa tgccggctcc aggcgctcgc cgaccggaaa tccgcgcact
    10021 tctatgagta gttggaagga gtaggctatg ggcaacggcg ggatcgagtg ggtcgtggcc
    10081 gtcaacctgg tcatctggac cggactgttc atctacctgc tccgcctcgg caagaagctg
    10141 aggaagctgg agaaagagcc atgaaacgga atcgtgtcta cctgttcggc ggcttcctgc
    10201 tgctcgcctt cgccggcttc tccttctcgg ccttcaagga gacgctgacg ccctacgtct
    10261 cctatgagga ggcgcggcgc ggcggccagg tgcaggtggc cggcgccctg gtcaagggga
    10321 gttcctccta cgagaccgcc aaggacgcct tctacttcac catccacgac ccggaggacg
    10381 ccaacgagac catccgcgtc cgctatcacg gtctgaagcc ggccaacttc gaggaggcga
    10441 tcagcatcgt ggcgatcggc agctaccagg cgggcgccaa cgagttcacg gccgacaagc
    10501 tgctggtcaa gtgcccgagt aaataccagg gcgccgaagt caaggagtac acctaggccg
    10561 tggactacgt ctcacagctc tacctgccgg gcgccctcac cctctggtgc gcgaccctgt
    10621 tcgccctgtc gagcctctgg ggctacgcgc tggtgctgcg cggcgacgcc gggtcgctca
    10681 ccttcgcccg ccgcgcctac ggcttcttca ccctgtccat cgtcgccgcc tcggcgctcc
    10741 tcgggctgct gctcttgatg cgcgacttcc ggatcgagta cgtctaccag tactcgggcc
    10801 tcgacctgcc ggcgcactac cagttcgccg ccttctgggc cggccagaag gggagcttcc
    10861 tcatctggct cttctggggc gcgctgatcg gcctgctggt ccgccgcacg acgcgcgaag
    10921 acgagcccgc ggtcatgggg atctacaccc tgaccctgct gggcctcctc ttcatcctgg
    10981 tgcgcgagaa ccccttcgtc atgctgcgcg agacgccggt ggacggccag ggcctgaacc
    11041 cgctgctcca ggacgactgg atgacgatcc acccgcccat catgttcgtg ggctacgcgg
    11101 cgaccgccat ccccttctcc ttcgccatgg cctcgctctg gcgccgcgac tacaccggcg
    11161 gctgggcgcg gcgcgccttc ccgtgggcgc tgggcggctt cctggtgctc ggcgccgcca
    11221 tcctgatggg gggctactgg gcctacaaga cgctcggctg gggcggctac tggggctggg
    11281 acccggtgga gaacgcgtcg ctgatcccct ggatcctcgg caccaccctg atccacggcc
    11341 tctacatgga gcgcagcaag gggcgttacc gccgggtgaa ctacatcctg gcggcgctca
    11401 ccttcctgtc ggtcctctac ggcaccttcc tcacccgctc gggcgtgctc gccgacttct
    11461 cggtgcacag cttcgtcgac ctcgggatct ccggctggct gatcgcggtc atggccacct
    11521 tcatcggcat gtcgatctat ctcctggcga gccggctccg cgaggtgcc
//
LOCUS       DASPJI010000013         8657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_630168, whole genome shotgun sequence.
ACCESSION   DASPJI010000013 DASPJI010000000
VERSION     DASPJI010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8657)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8657
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630168"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..115
                     /gene="rrf"
                     /locus_tag="VEL74_00405"
     rRNA            <1..115
                     /gene="rrf"
                     /locus_tag="VEL74_00405"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(174..1541)
                     /locus_tag="VEL74_00410"
     CDS             complement(174..1541)
                     /locus_tag="VEL74_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HYG61014.1"
                     /translation="MRRLPFLLTTVLIVAALALTGRTAQAFCGFYVAKADTKLFNQAS
                     QVVLVRDGDRAVLTMANDFKGDPKEFAVVIPVPTLIQRGQIHVANQALLDHLDAYSAP
                     RLVEYFDENPCEQRVFAQAAPAPAAPTAGAAVDARERAHSLGVTVEAEYTVGEYDIVI
                     LSAKESQGVETWLVETGYRIPQGTSRILGSYLKQGMKFFVAKVNLEEQSKLGFSYLRP
                     IQVAYESPKFMLPIRLGMVNAAGPQELFVFALTRKGRIETTNYRTVRLPSDQEVPLFV
                     KNEFGDFYKAMFSQQVRKQDMRAVFLEYAWDMAWCDPCAADPLSREELRELGVFWLAA
                     DRRRPDLPPPGGGAQDVFLTRLHLRYDGEHFPEDLVFQETADRQNFQGRYILRHPWKG
                     NDRCEEATSYRRGLAKRYESEAQTLASLTGWQVDKIRGKMNLKAGAPAAPPEEEPWWK
                     AIWKN"
     gene            complement(1538..2395)
                     /locus_tag="VEL74_00415"
     CDS             complement(1538..2395)
                     /locus_tag="VEL74_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HYG61015.1"
                     /translation="MRHLVALSRGVRDPRHYQIAVLGSLLLYGITRLDLEVRPVYAAA
                     MLVTALSVQYACGRLRKLPAFDPRSALISGLSLCLLLRTNSLTLAVAAAVVTIGSKFV
                     LRVRGKHVFNPTNFGIVALMLVTGKIWVSPGQWGSAAFFSFLVACLGGLVVNRAARSD
                     VTYAFLAFYLAVLFGRALWLGQPASIPLHQLGSGAFLIFTFFMISDPKTTPDSRAGRI
                     LFALLVALGAGFVHFVLYRPNGLILSLAFLSPLVPLIDRLLPGERYAWRRGDITGPEL
                     PLVPERRFA"
     gene            complement(2477..3184)
                     /locus_tag="VEL74_00420"
     CDS             complement(2477..3184)
                     /locus_tag="VEL74_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HYG61016.1"
                     /translation="MLHAFLPACCLACGQPLPARGARLGLCGGCRARLRRLPVARCAA
                     CACPLETANPPAGWLCSPCRVHTPAFDRLLAPWTYDPPLDQVVQALKFARLDYLGRHL
                     AQALVEELGTALVAFDRVVPVPLHWRRQLARGYNQAERIAQPMAGLLGIPCGSDLRRR
                     RATPPQTSLGRDSRRENLRGVFFVPRPARVRGRRVLLVDDVATTGATLDAAARALRAA
                     GAAAVTAVVAARTPIQG"
     gene            3343..3894
                     /locus_tag="VEL74_00425"
     CDS             3343..3894
                     /locus_tag="VEL74_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61017.1"
                     /translation="MNRDHRLLAAFFLVSALLLSPAISGGQELYTYTVDLLGGIGGSP
                     DVEQGDGFDNTGLQVNLSMVTEPGTHVGVRVGKLGLDSDEFFGSFEEAELSYLTLAGE
                     YRFPQTYYESGIYIGLGGYRLEGTRFGEDDDDTAPGLSLGVTGEFKFNRWLGLLIELS
                     GHYVDFDEAQLFGMAHGGLSVHF"
     gene            complement(3907..4494)
                     /locus_tag="VEL74_00430"
     CDS             complement(3907..4494)
                     /locus_tag="VEL74_00430"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYG61018.1"
                     /translation="MHFPAAKAAEPPSPPEAVARREPGLGRQANLVVAVLAVLALAVL
                     FWPRRDDRFDTAGLVDASGQAATLEPLLTPVTLVHFWATWCAPCMDEIPALRRLQDDM
                     EKNDDFRVVMIAVSDSTDKVQAFLGSGAGSVLYDPDWKVANSYGTDKLPETYLVVNGH
                     VVEKFVGQTDWDAPALRQKLAARLSGGDRTGPTGS"
     gene            4706..>8657
                     /locus_tag="VEL74_00435"
     CDS             4706..>8657
                     /locus_tag="VEL74_00435"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG61019.1"
                     /translation="MLELAPDLSHLDRLLGDVLLEGEIARTSRAVVYRVHTGPLGGAG
                     NRPLALKVALEASDADDLARFLHEVRLLSEVRHPNVVEVHDFGVLPGDFPFLTMELAG
                     GGLPEKLRRGGWDDLFDVAIQAAAGLAHIHRQGVVHMDIKPQNLGLLESEGGRQPVVK
                     ILDFGLAQAVRGPLDRRIRGTLAYTAPEILLQDSYDHRADLYSLGMTLLELSTGVLPS
                     AGDELAAIRFHLEGELPDPRVLRPDMPPALARILARLLRRDPGERYASAGRLLADLGT
                     ASGRRIDGATLSLSEGTVLASRLVGREESLGWLRSALAAAAEGRGGAVVVEGAEGVGK
                     SRLLREFRLLAAVTGARVARGRGIAERGQPLGSFLEALGSQGVAIEVQGEMPDAEADR
                     RERYRLYQEVALRIAACAEPGPPLVLLLDDVHLDGRESEELLIYLAEELKSRRVLVVA
                     SRRIPEAPRELGSVQTLRLAALDPAETAALVDACLGTSGLSANFYGWIFEQTGGLPGE
                     TQLLLRHLVDDRVLLYRDGEWKPSLAALSRWVSAAGGAEEQDRQRLAALPAAEREVLG
                     AAAVIDAPFSLSLVAALLEAEPQAVYERLSNLVSQGSLERLQEAEGSLYALSQRRFQR
                     LFYTELDGERRIALHRRLAGLLQERLERGEGDLVTAIADHFWRGGERARSLPYLLRAA
                     ETVLGIYGYSQAALFYGRAAEAAAEAGDRELEIRALSSQAEALAAAGSYPRASRVYQD
                     LLERPELRSLRARDRVLLARLQLRKGRLHTRLGEHEAALESCEEGLRQLTGIAEPELE
                     IELLHGKALALRDLSDWTAAFSAARSALAQAGREGLQRPRAVLLNALGMFYWARGDWR
                     RAGRLVRRALWVAEQLGDEALGLKLRNNLGMVLWKTGDHERAIGYYRSNLAASERTRD
                     LWAQLFSLNNLGILECGRGNWKAAREPFSRSLEITRRLGARENEVRVRLNLGEVEEVL
                     GNWPRAQRHYERALRLLEDSPEHPERFAALAQLASLERKRGRSAEAERRAREAVAGAE
                     GLGDRDLLAQGSLQLGLIEKDRESHDKAEAHLRRAMAEAETAETPQTAARAYLSLADL
                     ALRQGEVAAAEGHAREARTRIEALGDRLTEAKLLTVEARLASARDDVEQAERLFASGI
                     RLLEELEAPYEHARSLYEWGLRTWNVDTALRRLRRALVGFERLGAETECRRTSGALER
                     IREHQRLGGGRGASNVLYEVLKVINSTLDLQKVLNRTMDLVLERLGAERGMIVFSDPL
                     TRDLEVAVARNLGRDDEAEGQKLSESVVRRVIDSREPVLAVDALSDGRFVASDSI"
BASE COUNT         1299 a         2886 c         3189 g         1283 t
ORIGIN      
        1 cggtggctct agcgaggggg acacacccgt tcccattccg aacacggcag ttaagcccct
       61 cagcgccgat ggtactgcat gcgtaagtgt gtgggagagt aggtcgttgc cgggaagctg
      121 attttctaga cggccctcga gaatcctcga gggccgtcgc cttatctacc cggtcaattc
      181 ttccagatcg ctttccacca gggctcctcc tccgggggcg ccgccggtgc accggccttc
      241 agattcatct tgccgcggat cttgtcgacc tgccagccgg tcagggaggc cagcgtctgg
      301 gcctcgctct cgtagcgctt cgcgagcccg cggcgatagc tcgtggcctc ctcacagcgg
      361 tcgttgccct tccaggggtg gcgcaggatg tatcgtccct ggaagttctg ccggtcggcc
      421 gtctcctgga agaccaggtc ctccgggaag tgctcgccgt cgtagcgcag gtggaggcgg
      481 gtgaggaaga cgtcctgggc cccgcctccc ggcggcggca ggtccgggcg gcgccgatcc
      541 gcggccagcc agaagacccc cagctctcgc agctcctcgc gggacagcgg gtcggccgcg
      601 cagggatcgc accaggccat gtcccaggcg tactcgagga agacggcgcg catgtcctgc
      661 ttccggactt gctgcgagaa catcgccttg tagaagtcgc cgaactcgtt tttgacgaag
      721 agcggcacct cctggtccga gggcaggcgg acggtgcggt agttggtggt ctcgatccgc
      781 cccttgcggg tcagcgcgaa gacgaagagc tcctgcgggc ccgcggcgtt gaccatcccc
      841 aggcggatgg ggagcatgaa cttgggcgac tcgtaggcga cctggatcgg ccgcaggtag
      901 ctgaagccga gcttcgactg ctcttcgagg ttcaccttgg cgacgaagaa cttcatcccc
      961 tgcttgaggt agctgccgag gatccgggag gtcccctgcg ggatgcggta gccggtctcc
     1021 acgagccagg tctctacccc ctgactctcc ttggcggaga ggatgacgat gtcgtactcc
     1081 ccgacggtgt actcggcctc gacggtcacc cccaggctgt gggcgcgctc ccgggcgtcc
     1141 acggccgctc cggcggtcgg cgcggcgggg gccggtgccg cctgggcgaa gactctctgc
     1201 tcgcaagggt tctcgtcgaa gtactccacg agccgcggtg ccgagtaggc gtcgaggtga
     1261 tcgaggagcg cctggttggc gacatggatc tgtccccgct ggatcagggt ggggaccggg
     1321 atcaccaccg cgaactcctt gggatctccc ttgaaatcgt tggccatggt caggacggcg
     1381 cggtcgccat cccgcaccag caccacctgc gaagcctgat tgaagagctt ggtatccgcc
     1441 ttggccacgt agaagccaca gaaggcctgg gccgtacggc cggtgagcgc cagggccgcc
     1501 actatcagga ccgtggtcag gaggaagggt agccgtctca tgcgaatctc ctttccggga
     1561 cgagcggcag ctcgggcccg gtgatgtcgc ctcgccgcca ggcatagcgc tcccccggca
     1621 ggagccggtc gatcagcggc accagcgggg agaggaaggc gagggagagg atcaggccgt
     1681 tgggccggta gagcacgaag tggacgaagc ccgcgccgag ggcgaccagc agagcgaaaa
     1741 ggatgcggcc ggcccgggaa tcgggcgtgg tcttggggtc cgagatcatg aagaaggtga
     1801 agatcaggaa ggcgccgctc ccgagttggt ggagcgggat gctcgcgggc tggcccagcc
     1861 agagcgcccg gccgaagagc accgccaggt agaaggcgag gaaggcatag gtgacgtcgc
     1921 tccgcgccgc ccggttgacg accaggccgc ccaggcaggc caccaggaag gagaagaagg
     1981 cggcgctccc ccactggcct ggcgagaccc agatctttcc ggtgacgagc atcaaggcca
     2041 cgatcccgaa gttggtcggg ttgaagacgt gcttgccgcg gacgcggagg acgaacttgc
     2101 taccgatggt gaccacggcg gcggccaccg ccagcgtgag ggagttggtg cggagcagga
     2161 ggcacagcga gagtcccgag atgagggcgc tccgcggatc gaaggccggc agcttccgaa
     2221 gccggccgca ggcgtactgg accgacaggg cggtcaccag catcgccgcc gcgtacaccg
     2281 gccggacctc gaggtcgagc cgggtgatgc cgtagaggag aagcgatccg agcaccgcga
     2341 tctggtagtg gcgggggtcc cggactccac gcgagagggc aaccaagtgg cgcacgtcat
     2401 cgacctcctg gggcacctcg gtcttggagg gcctctcctt tcagacacgc cgatgcacgg
     2461 aacggcaacc ggccgctcaa ccctggatgg gggtgcgggc ggcgacgacg gcggtgacgg
     2521 cggcggcacc cgcggcccgc agggcgcggg cggcggcatc cagggtggca ccggtggtgg
     2581 cgacgtcgtc gactaggagc accctgcgtc cgcggacccg ggcgggccga ggcacgaaga
     2641 agacgccgcg aaggttctcg cgcctggagt cccggccgag ggaggtctgg ggcggcgtcg
     2701 cccgccggcg gcggaggtcg gagccgcagg ggatccccag caggccggcc atgggctggg
     2761 cgatccgctc cgcctggttg tacccgcgcg ccagttgccg ccgccagtga agagggacgg
     2821 ggaccacgcg atcgaaggcc acgagagccg tcccgagctc ctccacgagc gcctgcgcca
     2881 agtggcggcc caggtagtcc aggcgggcga acttgagcgc ctgcaccacc tggtcgagcg
     2941 gaggatcgta ggtccatggg gcgagcagcc ggtcgaacgc cggcgtgtgg acgcggcagg
     3001 gagagcagag ccatcccgcg ggagggttgg cggtctccag ggggcaggcg caggccgcgc
     3061 acctcgccac cggcagccgg cggagccgcg cgcggcagcc gccgcagaga cccagcctcg
     3121 ccccccgggc cgggagcggc tggccgcagg ccaggcagca ggcgggcagg aacgcatgga
     3181 gcaagcggtc gagccaacga cgctgctccg gatcgtcggg tgtcggcgtg acgccggagg
     3241 ggggacgtac gtataaagag tagtgggtca cggcacgtag tggggcgcga ggttatactc
     3301 cctggcggct cgcgagggtg ccggcctcaa aggaggaaaa acttgaaccg cgatcatcgc
     3361 cttctggctg cgttctttct ggtctccgct ctgctcctct cgcccgcgat ctcgggtggc
     3421 caggagctct acacctacac tgtcgacctg ctcggcggca ttggcggctc gcccgacgtg
     3481 gagcagggcg acggcttcga caacaccggc ctgcaggtca acctgagcat ggtcaccgag
     3541 cccggcactc acgtcggggt tcgcgtcggc aagctcggtc tcgacagcga cgaattcttc
     3601 gggtcgttcg aggaggccga gctctcctac ctcactctcg ccggcgagta ccggttcccg
     3661 cagacctact atgagtccgg aatctacatc gggctgggag ggtatcgcct cgagggtacc
     3721 cgcttcgggg aggacgacga cgacaccgcg ccggggctct ccctgggcgt caccggcgag
     3781 ttcaagttca accgctggct gggactgctg atcgaactgt ccggtcacta cgtcgacttc
     3841 gacgaggccc agctcttcgg tatggcgcac ggggggctca gcgtccactt ctaggccggt
     3901 gaggcttcac gagcccgtcg gccccgtcct gtcgccgccc gacagccggg ccgccagctt
     3961 ctggcgcagg gcgggggcat cccagtcggt ctgcccgacg aacttctcga cgacgtggcc
     4021 gttgacgacc aggtaggtct cggggagctt gtcggtgccg tagctgttgg ccaccttcca
     4081 gtcggggtcg taaagcaccg acccggcgcc ggaccccagg aaagcttgca ccttgtcggt
     4141 cgaatcggag accgcgatca tgacgacgcg gaagtcgtcg ttcttctcca tgtcatcctg
     4201 cagccggcgc aaggcaggga tttcgtccat gcagggggcg caccaggtgg cccagaagtg
     4261 gaccagcgtc accggcgtga ggagcggctc cagcgtcgcc gcctggccgc tggcgtcgac
     4321 cagccccgcg gtgtcgaagc ggtcgtcccg gcgaggccag aagaggaccg cgagcgcgag
     4381 gaccgccagc accgccacta cgaggttggc ctggcgtcct agccccggct cgcgccgcgc
     4441 cacggcttca ggcggtgacg gcggctcggc ggctttggca gcagggaaat gcatgagaaa
     4501 actccggtgt tttgaacatc gggtcaactt ttgacccgaa cgacctgtct ctctagaata
     4561 ctctccggcc ccgattgtac agcgaaggga gagaagggga tgcgtggtag acggagcgac
     4621 tgctctgacc ggcaaggctc ccctttgacg tcctggaagc actctcccat gtagcagcgc
     4681 tcgtgcctcg gccgacgcga ccgccatgct cgaactggct ccagacctct ctcatctcga
     4741 ccggctgctc ggcgacgtgc tgctcgaggg agaaatcgcc cgcacctcgc gggccgtggt
     4801 ctatcgtgtc cacaccggtc ccctgggtgg cgcgggcaac cggcccttgg cgctcaaggt
     4861 ggcgctcgag gcgagtgatg ccgacgacct ggcgcggttc ctccacgagg tccggctcct
     4921 ctcggaggtc cgccacccca acgtcgtcga ggtccacgac ttcggagtgc tgccggggga
     4981 cttccccttt ctgacgatgg agctggccgg gggtggcctg ccggaaaagt tgcggcgggg
     5041 gggctgggac gatctcttcg acgtggcgat ccaggcggcg gcgggcctgg cccacatcca
     5101 ccggcagggt gtcgtgcaca tggacatcaa gccgcagaac ctgggccttc tcgagagtga
     5161 aggggggcgg cagccggtcg tcaagatcct cgacttcggt ctggctcagg cggtgcgtgg
     5221 ccctctcgac cgccggatcc ggggcacgct ggcctacacg gctcccgaga tcctgctgca
     5281 ggacagctac gaccaccgtg ccgacctcta ctccctcggc atgacgctgc tcgagctgtc
     5341 gacgggtgtc ctcccctcgg cgggggacga gctggcggcg atccgcttcc atctggaagg
     5401 ggagcttccg gacccccggg tgctgcggcc cgacatgccg ccggccctgg cgcgcatcct
     5461 cgcccgcctg ttgcggcgcg accccggcga gcgttacgcc tcggcggggc ggcttctcgc
     5521 ggacctcggg accgcctccg gtcggcgcat cgacggcgcg accctgtccc tgtcggaggg
     5581 cacggtgctc gccagccgcc tcgtgggtag ggaggagagc ctcggctggc tgcggagcgc
     5641 gctggcggcg gcggcggagg gcaggggcgg cgccgtggtg gtcgaaggcg cggaaggggt
     5701 cggcaagagc cggctgttgc gggagttcag gctgctcgcc gcggtcaccg gggcgcgggt
     5761 ggcgaggggc cggggcatcg ccgagcgcgg ccagcccctg gggtcgttcc tcgaggcgct
     5821 gggctcgcag ggagtcgcga tcgaggtcca gggggagatg cccgacgcgg aggcggaccg
     5881 gcgcgagcgc tatcggctct accaggaggt cgcgctccgc atcgccgcct gcgcggagcc
     5941 ggggccgcct ctcgtcctcc tgctcgacga cgtccatctg gacggccgcg agagcgagga
     6001 gctgctgatc tacctggccg aggagctcaa gtcccgccgc gtgctggtgg tggcgagccg
     6061 gcggattccc gaagcgccgc gggagctggg ctcggtccag acgctcaggc tggcggcgct
     6121 ggatcctgcc gagaccgcgg ccctggtcga cgcctgcctg ggaacctccg gcctctccgc
     6181 gaacttctac ggctggatct tcgagcagac cggcggcctc cccggcgaga cccagctcct
     6241 gctccgccat ctggtggacg accgggtgct gctctatcgc gacggggagt ggaagccgtc
     6301 cctggccgcc ctctcccgct gggtgagcgc ggccggcggc gccgaggagc aggaccgcca
     6361 gcgtctcgcc gcccttcccg cggccgagcg ggaggtgctg ggcgccgcgg ccgtgatcga
     6421 cgcccccttc tcgctgtccc tcgtggcggc gcttctggag gccgagccgc aggcggtcta
     6481 cgagcggctc tccaatctgg tctcccaggg ttccctcgag cggctgcagg aggccgaagg
     6541 ctcgctctac gcgctcagcc agcgccgctt ccagcggctc ttctatacgg agctcgacgg
     6601 agagcggcgg atcgcgctcc accgccggct ggcgggtctg ctgcaggagc gtctggaacg
     6661 aggcgagggc gatctcgtta ccgccatcgc cgatcacttc tggcgcggcg gcgagcgggc
     6721 gcggagcctt ccttacctgt tgcgggcggc ggagacggtc ctcggcatct atggctactc
     6781 gcaggccgct ctcttctatg gacgggccgc cgaggccgcg gccgaagccg gcgaccggga
     6841 gctggagatc cgtgcactct cctcccaggc ggaggcgttg gccgccgcag gatcgtatcc
     6901 acgggcgtcg cgggtctacc aggacctgct ggagcggccg gagctgcgct cgctacgcgc
     6961 ccgcgaccgc gtcctgctcg cccgcctcca attgcgcaag gggcgtctcc acacccgcct
     7021 gggggagcac gaggcggcgc tggagagctg tgaggagggt ctgcgccaac tcaccgggat
     7081 cgcggagccg gagctcgaga tcgagctcct tcacggcaag gccctggccc tgcgcgacct
     7141 ctccgactgg acggcggcgt tctccgcggc ccgttcggcg ctggcgcagg ccggccggga
     7201 ggggctccag cggccgcgcg ccgtcctgtt gaatgccctg ggcatgttct actgggcccg
     7261 gggcgactgg cgccgcgcgg ggcgcctggt ccgccgggct ctgtgggtgg ccgagcagct
     7321 gggggacgag gcgctcggcc tcaagctccg caacaacctc ggcatggtgc tgtggaagac
     7381 cggcgaccac gagcgggcga tcgggtacta ccggagcaac ctcgcggcga gcgagcgcac
     7441 ccgggacctc tgggcccagc tcttctcgct caacaacctc ggcatcctgg agtgcggccg
     7501 tggcaactgg aaggccgcgc gcgagccgtt ctcccgcagt ctggagatca cgcgccggct
     7561 cggcgcccgc gagaacgagg tgcgggtgcg cctcaatctg ggggaggtcg aggaggtgct
     7621 cggcaactgg ccccgggcgc agcggcacta cgagagggcg ctacgcctgc tggaggactc
     7681 gcccgagcac ccggagcgtt tcgcggccct ggcccagctc gcctccctgg agcgcaagcg
     7741 ggggcggagc gcggaagcgg agcgccgggc gcgcgaggcg gtcgccgggg ccgaggggct
     7801 cggggaccgg gacctgctcg cgcagggctc cctgcagctc gggcttatcg agaaggatcg
     7861 cgagtcccac gacaaggcgg aggctcacct gcgccgcgcc atggcggagg cggagactgc
     7921 cgagaccccc cagaccgccg cgcgggccta tctgtcgctg gccgatctgg ctctgcgcca
     7981 gggagaggtc gcggccgccg agggccatgc gcgcgaagca cggacccgga tcgaggccct
     8041 gggggaccgc ctcaccgagg ccaagctcct cacggtcgag gcgcggctgg cgagcgcccg
     8101 ggatgacgtg gagcaggccg agcggctgtt cgcgtcgggc atacggctcc tcgaggagct
     8161 ggaggcaccg tacgagcacg cccggagcct gtacgaatgg ggcctccgga cctggaacgt
     8221 cgatacggcg ctccgccggc tccggcgggc gctggtgggc ttcgagcgtc tgggggccga
     8281 gaccgagtgc cggcggacca gtggcgccct cgagagaatc cgggagcacc agcggctcgg
     8341 cggcggccgg ggggcgagca acgtcctcta cgaagtcctc aaggtcatca actccaccct
     8401 ggacctccag aaggtgctca accgcaccat ggacctcgtg ctggagaggc tgggcgcgga
     8461 gcgcggcatg atcgtcttct ccgatcccct gacccgggat ctcgaggtgg ccgtggcgcg
     8521 caacctggga cgggacgacg aggcggaggg tcagaagctc tcggagagcg tggtgcgccg
     8581 ggtcatcgac agccgggaac cggtgctcgc cgtcgacgcg ctctccgatg ggcgcttcgt
     8641 cgcatcggac tccatcg
//
LOCUS       DASPJI010000014         2557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_287342, whole genome shotgun sequence.
ACCESSION   DASPJI010000014 DASPJI010000000
VERSION     DASPJI010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287342"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VEL74_00440"
     CDS             complement(<1..406)
                     /locus_tag="VEL74_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002735555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyalin"
                     /protein_id="HYG61020.1"
                     /translation="MGRSLFGWRPLAALALGLVVSTAAAAQPAYRVEDLNTTASGGTD
                     PWPTTGQFVELGGSVLFNVSDGVHGTEIWKTDGTAAGTSLVKDICPGACSAGSYLSMV
                     PLGPHVFFSADDGAHGAELWKTDGTAAGTVMVK"
     gene            558..2063
                     /locus_tag="VEL74_00445"
     CDS             558..2063
                     /locus_tag="VEL74_00445"
                     /inference="COORDINATES: protein motif:HMM:NF027900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HYG61021.1"
                     /translation="MTLSLPRLVLLAVVLSLAPFAGAHAVARFGTAEILLRADRTYNG
                     SSGTPNPFTDVALTAQVTSPTGRTFTVDGFFDGDGASGSTGNVFKLRVPADEPGTWSW
                     RTASNNAGLNAKSGTFSCTGTITGRFAGGPIVENPANPRTFMHQYGEPVYLIGKFLDV
                     AAPTPIQYTHTMLSENLTEANRQAMLDRHVGMGLNKMAVYLANKGDYGGTVPTTPWVG
                     TAASNDKMRFDLARWRTYEAWVIKMRDAGLVAQLWFFADDSGFGDLPDADRKRLIRYA
                     MARLSGYVNTMFTVMLEWQEGWTATEVDTTANYLHQWNPWAGPLSLLGMPGDFSFPNA
                     AWADYMQIQPGNGVGYAQVHTSSLHNRGLAAKPLIVEEFATGAESMGNRQKAWAAFMA
                     GPAGSGTGASLQHLARFAALVDFERMSPADGLVTSGAAYGLAESGRAYVFYLWNGGTV
                     TVNLSGATGTFIAEWYDPRTGVFRAAPAASGGGPRTYTAPASGDWTLYLHR"
     gene            complement(2064..>2557)
                     /locus_tag="VEL74_00450"
     CDS             complement(2064..>2557)
                     /locus_tag="VEL74_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61022.1"
                     /translation="AAGPPVEAAREETFFDSVEVRVVNVEVFVTGRDGKRITGLTRDD
                     FEGRELVPLTVKLPLSKLVLLPQGAFHEGRVTIQLAVRDPKGSHSGINEVQVPVRIPN
                     EQLLTALSQHAGYRTKVSLRPSSHVIAVSVRDELGNAESTVLTPYEPPQGAEVLAANR
                     AGR"
BASE COUNT          406 a          896 c          865 g          390 t
ORIGIN      
        1 ccttgaccat gaccgtgccg gcggcggtgc cgtccgtctt ccacagctcg gctccgtggg
       61 caccgtcgtc ggccgagaaa aagacgtggg ggccgagagg caccatcgag aggtacgagc
      121 cggccgagca ggcgcccggg cagatgtcct tgaccaggga ggtgcccgcg gcggtgccgt
      181 ccgtcttcca gatctcggtg ccgtgcacgc cgtccgagac attgaaaagg acggagcctc
      241 ccagctccac gaactggccg gtcgtcggcc acgggtcggt gccgccggag gcggtcgtgt
      301 tgaggtcctc gacgcggtag gcgggctggg ccgccgcggc agtggaaacg accagaccta
      361 gagcgagcgc ggcgagcggc cgccagccga agagactacg ccccatcgtc agacctccct
      421 taaaattctg gcgtctatga taactcttcg cgctggacat caacacgggg catggatctt
      481 gccctcccct ctatcgagct cccggcgcgt ctccgatacg gagccgcgcc aggtctgtaa
      541 gctcgaggag tcgcagtatg accctgtccc tgccccgcct ggttctgctc gcagtggtcc
      601 tctccctggc tcccttcgcc ggcgctcacg cggtggcccg gttcggcacg gccgagatct
      661 tgctgcgggc cgaccgtacc tacaacggct cgtccggcac ccccaacccc ttcaccgatg
      721 tggccctcac cgcccaggtg acctcgccca ccggccggac cttcaccgtc gacggcttct
      781 tcgacggcga cggcgccagc ggctcgaccg gaaacgtttt caagctccgg gtccccgccg
      841 acgagccggg cacgtggagc tggcggacgg ccagcaacaa cgccggcctg aacgccaaga
      901 gcggcacgtt cagttgcacg ggaacgatca ccggccgttt tgccggcggc ccgatcgtgg
      961 aaaaccctgc aaatccccgg acgttcatgc atcaatacgg cgagccggtc tatctgatcg
     1021 gaaagttcct cgacgtcgcc gcgcccacgc cgatccagta cacgcacacg atgctctccg
     1081 aaaaccttac ggaggcgaac cgccaggcga tgctcgaccg ccacgtgggg atggggctca
     1141 acaagatggc ggtctacctc gccaacaagg gggactacgg cggcaccgtg cccacgaccc
     1201 cctgggtggg cacggccgcg agcaacgaca agatgcgctt cgatctcgcg cgctggcgga
     1261 cctacgaggc ctgggtgatc aagatgcggg acgcgggcct ggtggcccag ctctggttct
     1321 tcgccgacga ttccgggttc ggcgatctgc cggacgccga ccgcaagcgg ctgatccgct
     1381 acgccatggc gcggctgtcg ggctacgtga acacgatgtt cacggtgatg ctggagtggc
     1441 aggaggggtg gacggccacc gaggtcgaca ccaccgccaa ctacctccac cagtggaacc
     1501 cgtgggccgg tccgttgagc ctcctcggca tgccgggcga cttctcgttc cccaacgccg
     1561 cctgggccga ttacatgcag atccagccgg gcaacggcgt ggggtacgcg caggtccaca
     1621 cctcgtccct ccacaaccgg gggctcgcgg ccaagccgct gatcgtcgag gagttcgcca
     1681 cgggagcgga gagcatgggc aaccggcaga aggcctgggc ggccttcatg gccggcccgg
     1741 ccggctcggg caccggcgcg tcgctccagc acctagcccg cttcgccgcg ctggtcgatt
     1801 tcgagcggat gagccccgcc gacggtctgg tgacctccgg cgcggcgtac ggtctggcgg
     1861 agagcggccg cgcctacgtg ttctatctct ggaacggcgg cacggtgacg gtcaatctgt
     1921 caggcgcgac cggaaccttc atcgcggagt ggtacgaccc gcggaccggc gtgttccggg
     1981 ccgcccccgc ggcgagcggc ggcggcccga ggacctatac ggcaccggcg agtggagact
     2041 ggacgctcta tctccaccgc tgatcagcgg ccggcgcggt tggcggccag cacctccgct
     2101 ccctgcggcg gctcgtaggg agtgagcacc gtcgactcgg cgttgccgag ctcgtcgcgc
     2161 acgctcaccg cgatgacgtg cgacgacggc cgcagcgaca ctttggtgcg gtagccggcg
     2221 tgctggctga gggcggtcag caactgctca ttggggatgc gcaccggcac ctgcacctcg
     2281 ttgatcccgg agtggctccc cttggggtcg cgcaccgcga gctggatggt gacgcgcccc
     2341 tcgtggaagg cgccctgcgg cagcaggacg agcttggaca gcggcagctt cacggtcagc
     2401 gggacgagct cccgtccctc gaaatcatcc cgggtcagcc cggtgatccg cttgccgtcg
     2461 cggccggtga cgaagacttc gacgttgacg acgcggacct ccacggagtc gaagaaggtc
     2521 tcctcccggg cggcctccac cggcgggccg gcggcac
//
LOCUS       DASPJI010000015         4032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_802153, whole genome shotgun sequence.
ACCESSION   DASPJI010000015 DASPJI010000000
VERSION     DASPJI010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802153"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VEL74_00455"
     CDS             <1..362
                     /locus_tag="VEL74_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61023.1"
                     /translation="LAPAAPVTFRQAFDLLNTYHSRRDLVVLGVYGGPGLSVAGEVMP
                     RLPASVRSLWSAAASGSAVALRSTIAQQITQTLDVPIDGMVQIDLEVRRREPVLAGEG
                     NDTGAAVAVGTGGTEGR"
     gene            359..2605
                     /locus_tag="VEL74_00460"
     CDS             359..2605
                     /locus_tag="VEL74_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HYG61024.1"
                     /translation="MIRRAKIRLGAAALLGCVGLLAAGSPARGTDVRIFQTQSQVGFL
                     AGTLEGVSVDSLGRMELAPRVERVASLAEPFVLSAARLGAGNTGDSGWVIGTGNAGKV
                     LRVDARGKVTELFAAAEPEVFAVWADPDGTVFAGTSPGGKVYRIPAGGTAAKAEPFFD
                     SGETYVWSLRRAADGRLLVATGTQGKLFAVDAKGKGEVLYDSDDTHIRALELLPDGDL
                     LIGTAGEGLILRRSKTGEVRTLYDADEPEVVALAAGPDGICYAGVVSSEASLVDLSKT
                     GTAGTAAQSAQPQGQATVTVTAGDSTTTATTTTGTRREGATGPRSQVLSILPAGVVES
                     LWSFPDETVYSLLWSGDRLWIATGLEGKVYSYDAAQLLLEKDVDERQVVALLPGEPGP
                     AFATTNAAAFYRVTSGTERTGTYTSAALDAGQIARFGTFRWEGEAPAGSSVRLSFRSG
                     MSAEPDRTWSGWTDIKEGKEVALDGLPRGRYVQWRAQLQSGAGGSPRLYGTELSFRQE
                     NMSPKIQVLAALQPGQILVPANFNPSNQVYEPAHPNREGIFTTLEASSADEDRNRTKP
                     LWKQGFQTLQWTASDPNGDQLVYQLWFRPEGREVDEGWLQVAKDVEDAYVSFDATALP
                     DGVYRFKLAASDSPANEPESALTTERVSDPVVIDHTPPALGEVRREGVRLRVVVRDTA
                     SPIREAVYSVDAAEWKPARAADGLLDGRTETLVIEGGDPDEKAGLVLLRVTDAAHNVM
                     TFDLTGKR"
     gene            2602..3108
                     /gene="coaD"
                     /locus_tag="VEL74_00465"
     CDS             2602..3108
                     /gene="coaD"
                     /locus_tag="VEL74_00465"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HYG61025.1"
                     /translation="MSQRIAVYPGSFDPIHNGHLDIIERCRPLFDEVVVAVLQNEGKK
                     PLFSVEERVELIREMVAGSPQVRVESFSGLLVDFMDRIGARAVVRGLRAVSDFEYEFQ
                     MALMNRRLNKQVETLFMMPREDYSYLSSRLVKEVFALGGDLTGLVPEPVLARLRQHLR
                     PVQPAGPA"
     gene            3105..>4032
                     /gene="add"
                     /locus_tag="VEL74_00470"
     CDS             3105..>4032
                     /gene="add"
                     /locus_tag="VEL74_00470"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HYG61026.1"
                     /translation="MSIERFVLRMPKAELHVHLEGAMRPAVLLELARRNGIELPAKDE
                     AGLKRWFRFRDFDHFVQVYLACSRALRRPEDFHFLALDFLAEQAYQRVVYTEAHFTIG
                     THLANGSLGAEVLSALEEAIREGERRYGVMLRLIPDIVRNVPQQADGTLEWALAGRGR
                     GVVALGLSGSEARFANEPFRRHFEEARRVGLHRVAHAGEHAGPESIRSVLEVCGAERI
                     GHGVRAVEDSALVQELRAARIPLEVSPTSNVCLGVVANLESHPFDRLYRAGLRLSVNT
                     DDPSFFNTNLSREYLNLHRAFGYGPGQLAGFAL"
BASE COUNT          568 a         1393 c         1473 g          598 t
ORIGIN      
        1 cgctggcgcc ggcggcgccg gtgacgttcc gtcaagcgtt cgacctgctg aacacgtatc
       61 actcccggcg cgacctggtg gtcctgggcg tctatggcgg cccgggcctg tcggtggcgg
      121 gggaggtcat gccgcgcctg ccggcgtcgg tgcgctcgtt gtggagcgcc gccgcgtccg
      181 ggagcgcggt cgcgttgcgc tccacgatcg cgcagcagat cactcagacg ctggacgtcc
      241 ccatcgacgg gatggtgcag atcgatctcg aggtgcgccg ccgcgagccg gtgctcgcgg
      301 gcgaaggcaa tgacaccgga gcggcagtgg ccgttggaac cgggggcacg gaggggagat
      361 gatcagacgg gcgaagatcc gcctcggggc ggccgctctt ttagggtgtg tgggattgct
      421 cgcggcggga tcgcccgcgc gcggcaccga tgtcaggatt ttccagaccc agagccaggt
      481 cggcttcctc gccggcactc tggaaggggt gagcgtcgac tcgctgggcc gtatggagct
      541 ggcgccgcgg gtcgagcgcg tggcctctct cgccgagccg ttcgtcctct cggccgcccg
      601 tctcggtgcc ggcaataccg gcgacagtgg ctgggtgatc ggcacgggca acgccggcaa
      661 ggtgctgcgg gtcgacgccc ggggcaaggt gacggagctc ttcgccgccg ccgagcccga
      721 ggtcttcgcc gtctgggccg acccggacgg caccgtcttc gccggcacgt cgcccggcgg
      781 caaggtctac cgcatccccg cgggtgggac cgcggcgaag gccgagccct tcttcgactc
      841 cggcgagacc tatgtctggt ccctgcgccg ggcggcggac ggccgcctgc tcgtggccac
      901 cggcacccag ggcaagctct tcgccgtcga cgcgaagggg aagggtgagg tcctctacga
      961 cagcgatgac acccacatcc gcgccctcga gcttctgccg gacggcgacc tgctcatcgg
     1021 caccgcgggc gaggggctca tcctgcgccg gagcaagaca ggcgaggtcc gcaccctcta
     1081 cgacgccgac gagcccgagg tggtggcgct ggccgccggg cccgacggca tctgctacgc
     1141 tggcgtggtc tcctcggagg cgagcctggt ggacctctcc aagaccggta cggccggcac
     1201 cgcggcccag tcggcccagc cgcaggggca ggccaccgtc acggtgacgg cgggggatag
     1261 caccaccacg gccaccacga ccacgggcac ccgccgggag ggggccaccg gcccgcgcag
     1321 ccaggtgctg tccatcctgc ccgccggtgt ggtggagagc ctgtggagct tccccgacga
     1381 gacggtctac agcctcctct ggagcggtga ccggctgtgg atcgccaccg gcctcgaagg
     1441 caaggtgtac agctacgacg cggcgcagct cctgctggag aaggacgtgg acgagcgcca
     1501 ggtggtggcg ctgctccctg gcgagccggg ccccgccttc gccaccacca acgccgccgc
     1561 cttctatagg gtgacctcgg ggaccgagcg gacaggcacc tacaccagcg ccgccctgga
     1621 cgccggccag atcgcccgct tcggcacctt ccggtgggaa ggggaggcgc cggccggctc
     1681 gtcggtccgc ctgtcgttcc gcagcggcat gagcgccgag ccggaccgca cctggtccgg
     1741 ctggaccgac atcaaggagg ggaaagaggt ggcgctggac ggcctgccgc gcggccgcta
     1801 cgtgcagtgg cgcgcccagc tccagtccgg cgcgggcggc tcgccgcggc tctacggcac
     1861 ggagctctcc tttcgtcagg agaacatgag tccgaagatc caggtcctcg cggctctcca
     1921 gcccgggcag atcctggtgc cggccaactt caatccttcg aaccaggtct acgagccggc
     1981 ccatcctaat cgcgagggga tcttcaccac tctggaggcc tcctcggcgg acgaggaccg
     2041 caaccgcacc aagccgctct ggaagcaggg cttccagacg ctgcagtgga ccgcctccga
     2101 ccccaacggc gatcagctcg tctaccagct ctggttccgc cccgagggcc gggaggttga
     2161 cgaggggtgg ctccaggtgg cgaaggacgt cgaggacgcc tacgtcagct tcgacgccac
     2221 ggcgctgccc gacggcgtct accgtttcaa gctggcggcg tcggactcgc cggccaacga
     2281 gccggagtcg gcgctgacta cggagcgggt gagcgatccg gtggtgatcg accacacgcc
     2341 gccggctctg ggcgaggtgc ggcgggaggg ggtgcgcctg cgcgtggtgg tccgcgacac
     2401 ggcgagcccg atccgcgagg cggtctacag cgtcgacgcc gccgagtgga aacccgcccg
     2461 ggcggccgac ggcctgctcg acggtcgcac ggagacgctg gttatcgagg ggggggaccc
     2521 cgatgagaag gccgggctgg tgctgttgcg ggtcaccgac gcagcgcaca acgtgatgac
     2581 cttcgatctc acggggaagc gatgagccag cggatcgccg tctatccggg gtcgttcgac
     2641 cccatccaca acggtcacct cgacatcatc gagcgttgcc gtccgctgtt cgacgaggtg
     2701 gtggtcgccg tgttgcagaa cgagggcaag aagcccctct tctcggtcga ggagcgggtg
     2761 gagctgatcc gcgagatggt ggctggcagc ccgcaggtgc gggtggagag cttctcgggc
     2821 ctgctggtgg acttcatgga ccggatcggc gcccgggcgg tggtgcgggg gctgcgggcg
     2881 gtctcggact tcgagtacga gttccagatg gcgctcatga accggcggct caacaagcag
     2941 gtggagacgc tgttcatgat gccgcgcgag gactacagct acctgtcgag ccggctggtc
     3001 aaggaggtct tcgccctggg cggcgacctg accggcctgg tgccggagcc ggtgctcgcc
     3061 cgcctccgcc agcacctgcg tcctgtgcaa ccggccggcc ccgcatgagc atcgagcgtt
     3121 tcgtccttcg catgccgaag gccgagctcc acgtgcacct cgagggggcg atgcgtcccg
     3181 cggtgctcct ggagctggcg cggcgcaacg gcatcgagct gccggccaaa gacgaggccg
     3241 gcctcaagcg ctggttccgc ttccgcgact tcgaccactt cgtccaggtc tacctcgcct
     3301 gctcgcgggc gctgcgccgc ccggaggact tccacttcct cgccctcgac ttcctggccg
     3361 agcaggccta tcagcgggtg gtctacaccg aggcccactt caccatcggc acgcacctcg
     3421 cgaacggcag cctcggcgcc gaggtgctgt ccgcgctgga ggaggcgatc cgcgaggggg
     3481 agcggcgcta cggtgtgatg ctgcggctga ttccggacat cgtgcgcaac gtgccgcaac
     3541 aggcggacgg gacgctggag tgggcgctcg ccggccgcgg ccggggcgtg gtcgcgctgg
     3601 gtctttcggg cagcgaggcg cgcttcgcca acgagccgtt ccgccgccac ttcgaggagg
     3661 cgcggcgggt ggggctccac cgggtggccc acgccggcga gcacgcgggg ccggagtcga
     3721 tccgctcggt gctggaggtc tgcggcgccg agcgcatcgg ccatggggtg agggcggtgg
     3781 aggactcggc gctggtccag gagctgcggg cggcgcgcat cccgctggag gtctcgccca
     3841 cctccaacgt ctgcctcggc gtggtggcga acctcgagag tcaccccttc gaccgcctct
     3901 accgcgccgg tctgcgcctg tcggtgaaca cggacgatcc gtccttcttc aacaccaacc
     3961 tctcccgcga gtacctgaac ctgcaccgcg ccttcggcta cggccccggg cagctcgccg
     4021 gcttcgcgct cg
//
LOCUS       DASPJI010000016         2744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_802168, whole genome shotgun sequence.
ACCESSION   DASPJI010000016 DASPJI010000000
VERSION     DASPJI010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802168"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VEL74_00475"
     CDS             <1..898
                     /locus_tag="VEL74_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HYG61027.1"
                     /translation="KTVLERKAPPTFGVLIEIQDKDHLAVHHDVAEVVDRFLRDVPPR
                     PEIRTRNADGSVEIQKAEAPYRPPQPRSAPGRGRKGGRGGWSESRGPGRGERSESWEG
                     RGDRTNGRGPAGGANGPGPGAAHPPHAAPAGDGEPEPAAARPPKGRTARIYPYAVSKS
                     KLERAIRKLRVPAIMVEDLDDADLVVTLKAQERRQPRRLRDAAAGGVPVYIMKSNTIV
                     QAENFLRAVFDVGDVAADDEEALREVEDGIDEVLDNGEPVELPPRNNHLRRLQHQVVE
                     RYGLTSESKGDEPYRRVVIYPG"
     gene            963..2159
                     /locus_tag="VEL74_00480"
     CDS             963..2159
                     /locus_tag="VEL74_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018947738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG61028.1"
                     /translation="MFARTLEARFRALVGTFPAVFLTGPRQSGKTTLARTCFPDFHYV
                     SLEDLQNRQEVTEDPRGFLRRLEGRRGVILDEVQRTPELFSYLQGFLDEEKSGPMVLT
                     GSQHFLLSERISQSLAGRVAILELLPLSTAELAGRPALLPEELAEPADPRENKPPLRL
                     EEILFRGLFPRIHDRQLDASAWLVGYLRTYIERDVRLLTNVGNLEAFSRFLSLCAGRS
                     GQLLNSSALAVEAGITQPTARHWISVLQASYVILLLKPHHENFNKRLVKTPKLYFLDA
                     GLLCHLLGLRRPEDVHVHPLRGAIFETFIIGELYKTFLHHGQPAPLYFWRDAAGHEVD
                     ALLDLGTRRIPVEIKAGQTLAGDVFRGLDYYLKLAGGDTGVLIYGGDESYARKNHIVR
                     SWWHCG"
BASE COUNT          436 a          949 c          938 g          421 t
ORIGIN      
        1 gaagacggtg ctggaacgca aggcgccccc caccttcggc gtcctcatcg agatccagga
       61 caaggaccac ctcgccgtcc accacgacgt cgccgaggtg gtggatcgct tcctgcgcga
      121 cgtgccgccg cggccggaga tccggacccg caacgccgac ggctcggtgg agatccagaa
      181 ggccgaggcg ccgtaccgtc cgccccagcc gcggagcgcg ccgggcaggg gccgcaaggg
      241 cggccgaggc ggctggagcg agagccgggg gcccggccgg ggcgagagaa gcgagagctg
      301 ggagggacgg ggggaccgga ccaacggccg gggaccggcc ggcggggcga acggccccgg
      361 gccgggcgcc gcgcaccccc cgcatgccgc gcctgccgga gacggcgagc cggaaccggc
      421 cgccgcccgg ccgcccaagg ggcggacggc gcgcatctat ccgtacgcgg tctcgaagag
      481 caagctggag cgggccatcc gcaagctccg cgtgccggcg atcatggtcg aggacctcga
      541 cgacgccgac ctggtcgtca cgttgaaggc ccaggagcgg cgccagcccc gccgcctgcg
      601 cgacgccgcg gcgggcgggg tcccggtcta catcatgaag agcaacacca tcgtgcaggc
      661 ggagaacttc ctgcgcgccg tcttcgacgt gggcgacgtc gccgccgacg acgaggaggc
      721 actgcgcgag gtcgaggacg ggatcgacga ggtcctcgac aacggcgagc cggtcgagct
      781 gccgccgcgc aacaaccacc tccgccgcct ccagcaccag gtcgtggagc gctacggcct
      841 cacctccgag agcaagggcg atgagcccta ccgccgggtg gtcatctacc cggggtaggc
      901 cgtgcagatt caaaatccga ttttgaattg acacggcctc aagggtctgg taagcctggc
      961 ccatgttcgc tcgcaccttg gaggcccgct tccgcgccct cgtcggaacg ttcccggccg
     1021 tcttcctcac cggcccgcgg caatccggca agacgacgct ggcccggacc tgcttccccg
     1081 acttccacta cgtctccctg gaggatctcc agaaccggca ggaggtcacg gaagacccgc
     1141 ggggctttct ccgccggctg gaaggcagga ggggtgtgat cctcgacgag gtgcagcgca
     1201 cgccggagct cttctcctac ctccagggct tcctcgacga ggagaagagc ggaccgatgg
     1261 tgctcacggg ctcgcagcac ttcctgctct cggagcggat cagccagtcc ctggccggcc
     1321 gggtcgccat cctggagctt ctgccgctct cgacggcgga gctggccggg cggccggcgc
     1381 tcctgcccga ggagctggcc gagccggcgg acccgcggga aaacaagccg ccgctccgtc
     1441 tcgaggagat cctcttccgt ggtctcttcc cgcgcatcca cgaccggcag ctcgatgcga
     1501 gcgcctggct ggtcggttat ctgcgcacct acatcgagcg cgacgtccgt ctgctcacca
     1561 acgtcggcaa cctggaagcg ttcagccgct tcctgtctct ctgcgccggc cggtcggggc
     1621 agctcctgaa cagctcggcg ctggcggtgg aggccggtat cacccagcct acggcaaggc
     1681 actggatctc cgtgctccag gcgagctacg tcatcctcct cttgaagccg catcacgaga
     1741 acttcaacaa acggctggtc aagactccca agctgtactt cctggacgcc gggctcctct
     1801 gccacctcct gggactccgc cggccggagg atgtccatgt gcaccccttg cgcggcgcca
     1861 tcttcgagac gttcatcatc ggcgagctgt acaagacctt ccttcatcac gggcagccgg
     1921 ctcccctcta cttctggcgc gacgcggcag ggcacgaggt ggatgccctg ctcgacctcg
     1981 ggacgcggcg catcccggtg gagatcaagg cgggccagac gctcgccgga gacgtgttcc
     2041 gggggctcga ctattacttg aagctggccg gtggcgacac gggcgtcctg atctacggcg
     2101 gcgacgagag ctacgcgcgg aagaatcaca tcgtgcggag ctggtggcac tgcggatgag
     2161 gccgctgggt gcgcacgcag gctcctttct tgtgcgaaat gacggtcacc ctgtgcggaa
     2221 cggtcaacgt tccgtgcggg atgccggcct ctctgtgcgg gatcgcttcc acccggccct
     2281 ggcggacttc gtcttgtgcg gaacgatggc cgtcttgtgc gagatggtag ttgtctcgtg
     2341 cgagatggtc actaccttgt gcggattcat cgtcgcctga gccaagagga cgaccttctt
     2401 gtgcggagtg gtggccttgc ttgtgcgcgc aggtcgcctc tctgtgcgga gctgtctctt
     2461 ttttgtgcgg actttgggtc ccatcgcgct ggaacaggac cgtgacctac tcctgaaagg
     2521 caggaccagg tcacggcgtc tacttcagag aagaggcggg ccccgcgcgg cggggcccgc
     2581 cttcggggga gcgtcagacc tcgagaaagg cgacgcccgc ggggttcgac gggtccgcgt
     2641 cctcaacgat ccgcaccacc gaccagagcc gccagggcgc ccgcgggtcc ggctcctcct
     2701 ccagagacag ccaggaggca taccaccggc cgcggtcgta ccag
//
LOCUS       DASPJI010000017         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_230703, whole genome shotgun sequence.
ACCESSION   DASPJI010000017 DASPJI010000000
VERSION     DASPJI010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230703"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VEL74_00485"
     CDS             <1..610
                     /locus_tag="VEL74_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYG61029.1"
                     /translation="LTRSIRDLGLDHLERLPGTRREAERIADLDPGSFVALDFFAGRE
                     ILSHPAVRRARLLHLATHSLMNPRNPALSGIVLSLVDEQGRPQQGFLRADEIAELGLS
                     ADLVVLSACKTALGPEVRGEGLLSLARAFFEGGASRVVVSLWAVEDNASADLMTRFYD
                     GLLRQGLPAAAALRQAQLATRDAAEKNGRSEWAAFELQGDWR"
     gene            682..933
                     /locus_tag="VEL74_00490"
     CDS             682..933
                     /locus_tag="VEL74_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61030.1"
                     /translation="MGDNDSGTGTGTGTGTQKAGMSQAQPLPSTPEAHMGGSDNDSGT
                     GTGTGTGGSKGGFPKAEPLPGQAQSDGGSAAGSEETQAK"
     gene            930..2366
                     /locus_tag="VEL74_00495"
     CDS             930..2366
                     /locus_tag="VEL74_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61031.1"
                     /translation="MKTHGGMGGGLEDGARLVFNGVLNRGDGSGYLLAPRTPQELLAE
                     IGGGEPPSPPTVPRPLPSYIDANDLAKAGWGVIWAPGLDPRVKEALEPLLALRQREAG
                     PLYREVEARPGETFFQLLKRLGASVAPVDPKRLPYYLLVVGEPAEVPFELQYQLDVAH
                     AVGRLCFATPEEYGRYALGVVAVEENGVRRPRRLGIFAVQNQDDWATLSCREHLATPL
                     AESLRREVQDWEVDSCLGSEATKARLRQHLGGEATPAMLLTCSHGLMYQVGDPLQPTD
                     QGGLICADWPGPERWKERLPTEQYLAARDIADTDDLSGLISFQFACFSAGTPERDSFS
                     RARGSAVARLAARDMMAPLAQRLLGKPGGSALAVVGHVDQVFEASYLWPGVGRQLETF
                     EACLRGLMAGERLGLALEPFGHRHATIATFLTELLEQWRRQPETRPVGVSDALGEAFL
                     WLAHNDARGYLLLGDPAVRPAVGRDTLF"
BASE COUNT          380 a          890 c          901 g          394 t
ORIGIN      
        1 cctcacccga tcgatccgcg atctgggcct cgaccacctg gagcgcctgc caggcacccg
       61 ccgcgaggcc gagaggatcg ccgatctcga tcctggaagc ttcgtcgccc tggacttctt
      121 cgccgggagg gagatcctga gccaccccgc cgtgcgccgc gcccggctcc tgcatctggc
      181 cacccacagt ctgatgaacc cgcggaaccc ggcgctgtcc ggcatcgtgc tgtcgctggt
      241 agacgagcag gggcggcctc agcagggatt cctacgagca gacgagattg cggagctcgg
      301 cctgtcggcc gacctcgtgg tgctgagcgc ctgcaagacc gccttggggc ccgaagtccg
      361 gggcgagggg ctcctgagcc tcgcccgcgc cttcttcgag ggcggcgcca gccgggtcgt
      421 ggtcagtctc tgggctgtgg aggacaacgc ctcggccgac ctgatgaccc gcttctacga
      481 cgggctcctg cgtcagggac tgccggcggc ggcggccctg cgccaggccc agctcgccac
      541 gcgcgatgcc gcggagaaga acggtcgctc cgagtgggcc gcgttcgagc tgcaaggcga
      601 ctggcgctga gctggaaaat tgtcgcaccg tccgtctagt ggggtgtgct tgaatgcacg
      661 ccaaccacca aggaggttca gatgggcgac aacgacagcg gcaccggcac gggcacgggc
      721 accggcaccc agaaagccgg aatgagccag gctcagccac ttccatctac gcctgaggct
      781 cacatgggcg gtagcgacaa cgacagtggc accggcactg gaaccggcac gggtggttcg
      841 aagggtggct tccccaaggc tgagccactt cctgggcagg cccagagtga cggcggctcg
      901 gcggcgggat ccgaggagac ccaggcgaaa tgaagaccca cggtgggatg ggcggggggc
      961 tggaggacgg cgcgcggctg gtcttcaacg gcgttctcaa ccggggcgac ggcagcggct
     1021 acctgctggc gccccgcacg ccgcaggagc tgctggccga gatcggcggc ggtgagcccc
     1081 cttcccctcc caccgtaccc aggccattac cctcctatat cgatgccaac gacctggcca
     1141 aggcaggctg gggagtgatc tgggctccgg gcttggaccc gcgggtcaag gaggctctgg
     1201 agcccctgct ggcgctgcgc caacgggagg cgggccccct gtaccgcgag gtggaggccc
     1261 ggcccgggga gaccttcttc cagctcctca agcgtctcgg cgcctccgtg gcacccgtcg
     1321 accccaagcg gctgccctac tacctgctgg tggtagggga gccggcggag gtccctttcg
     1381 agctccagta ccagctcgac gtcgcgcacg cggtgggccg gctctgcttc gccacgcccg
     1441 aggagtacgg ccgctatgcg ctaggggtgg tggcggtgga ggagaacggg gtccgccgcc
     1501 cacggcgcct gggcatcttc gccgtccaga accaggacga ttgggccacc ctctcgtgcc
     1561 gggagcacct ggcgacgccg ctggccgaga gcctccggcg cgaggtccag gactgggagg
     1621 tcgactcctg tctcggcagt gaggccacca aggcgcggct acgccagcac cttggcggtg
     1681 aagccacgcc ggccatgctg ctgacctgca gccacggttt gatgtaccag gtcggcgatc
     1741 ccctccagcc cacggatcag gggggcttga tctgcgccga ctggccgggg ccggaacgct
     1801 ggaaggagcg cctgccaacg gagcagtacc tggcggccag ggacatcgcc gacaccgacg
     1861 atctgtcggg cctgatctcc ttccagttcg cctgcttcag cgccggtacg ccggagcgcg
     1921 actccttctc tcgtgcacgg ggcagcgccg tggcaaggct ggcggcgcgc gacatgatgg
     1981 cccccctggc ccagcggctc ctgggcaagc ccggcggctc cgccctggcg gtcgtgggcc
     2041 atgtcgatca ggtcttcgag gcctcgtacc tgtggcccgg agtgggccgg cagctcgaga
     2101 ctttcgaggc ctgcctcagg ggattgatgg cgggcgaacg cctgggcctc gccctggagc
     2161 cgttcggcca tcgccatgcc accatcgcca ctttcctcac cgagctgctg gagcaatggc
     2221 gccggcaacc ggagacccgc cccgtggggg tctccgatgc tctcggcgag gccttcctgt
     2281 ggcttgccca caacgatgcg cggggctacc tgctgctcgg cgaccccgcc gtccgtccag
     2341 ccgtcgggcg ggacaccctc ttctagcgcc atggcctccc gaggcgacat tcacgcgttg
     2401 gtcgccggag tggatctcta tctgcccggt ccggtccccg aggaggtcta tccgtcgctg
     2461 cggggagccg cggccgacgc cagcgaggtg gctctctacc tgacccggga gctggaggtg
     2521 ccggagggca atgtacgtct tctgacctcc atcgatgatg gcagt
//
LOCUS       DASPJI010000018         4144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_57955, whole genome shotgun sequence.
ACCESSION   DASPJI010000018 DASPJI010000000
VERSION     DASPJI010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57955"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VEL74_00500"
     CDS             complement(<1..276)
                     /locus_tag="VEL74_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG61032.1"
                     /translation="MIQIDLKGKRALVMGVSNARSLGWAIAERLRDAGAELAFSYQGE
                     RLKGDLEKLTAEMPGTRLYSCDVTREDELRAMFEDLRSTWGSLDYVVH"
     gene            312..1280
                     /locus_tag="VEL74_00505"
     CDS             312..1280
                     /locus_tag="VEL74_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61033.1"
                     /translation="MSTTRTLGILLVALAAGACTPPSPGPATGGDGSTESAQAGPGPA
                     RIGAPRTDAPETRAGADLRGTITQTSAVEPGGPGAPALGRVLVEGEVEPDTRYDRGWI
                     TVTEETRIVRREGMSDRPPSFLDLTVGLRVEVTFEGAVAKSLPLQATAREIVILGAAP
                     APGPGEEDGKTAGGEAADFKGTAGIIDKPRPDLPPATLRDVRTARQAGFDRVVFEFAG
                     GALPGYHLEYIDKPVRQCGSGEPVPLAGDAWLQVRLVPAYAHTDAGQATVQERERHLD
                     YPVLRELESTCDFEAHVEWVLGVASPNRYRVLELSDPTRLVVDVRH"
     gene            complement(1284..>4144)
                     /locus_tag="VEL74_00510"
     CDS             complement(1284..>4144)
                     /locus_tag="VEL74_00510"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYG61034.1"
                     /translation="PTPAAPAGEGVDGVVLVFLDGEGLGEGIAARLRAAGRSVATVQP
                     GGGFADLGGGAYAVRPGEAADYDALLAALPSEGREVGAILHLWSFGAPQPAPGAAPAG
                     SAADRAFYSPLFLAQALERRRIDERRLDLWLVSSHLHDVSGGDSVVPERALLLGPGKV
                     IPLELPGVVCHSLDLEAGGRLERTVDRLWAEVAAGSPEPVIAWRGRQRWLQSFEPVRL
                     EEGGPAAPRLRPGGVYVITGGLGGVGHAIARWLARSAGARLVLIGRTALPPREEWDGL
                     LARGGDGPLFRKLRRLRDLEEAGAEVLAVQADVTDPVGMVQVRALALERWGAVHGVIH
                     AAGIPGGGVIARKDRIAADVVLAPKVQGTLTLAATFPPEELDLFVLCSSLNSVIGAYG
                     QVDYCAANAFLDAFAGSRAGSAGLTVSIAWDAWTEAGMAVETAAPGARRLMDGLPVGA
                     AVDHPLLDRRVPAGAGEHAFVTRLDAARHWVLDEHRIQGTPVLPGTAYLELARAAFEQ
                     STGSAAAEIRQAAFVTPLAVPDGESRDVYTLLRRQGDAWSFRVASRLEAGGETRWQEH
                     ALGEIGPPAGSGAPVPSLRELLAELPVEPAAVDLGSSEVMSFGPRWRSLHRTAAADGR
                     FLAELRFPGEFAAELEALGLHPAVLDRATGFSQWLGPAADGVYMPFSYAGLRIERPLP
                     AELFSFIRTKQGSGEELHFEVVLFDPAGEVLVRIDDFAFRRVAARPNVPSRLEMVESA
                     PPAPVPVAAGEGLTSADGVEAFRRILSGGDLPQVMVSVRDLTARLAEAAALTGERILA
                     ELDRAGAAAPAGPRHGRPDLATAYVAPRTEAESWLAEIWGDLLGIDAVGIHDDFFELG
                     GDSVLSLRIVARARERGLALTPGRLFERPTIAGLAADLGAVAAEEPPAAQTAAPAAPA
                     AETPRPPAEGSVYDPARFPDADVSQRDLEALLAQLGGL"
BASE COUNT          565 a         1582 c         1528 g          469 t
ORIGIN      
        1 gtggacgacg tagtccaggc tcccccaggt gctccggaga tcctcgaaca tcgcccgcag
       61 ctcgtcctcc cgggtgacgt cacaggagta gagccgcgtt cccggcatct ccgccgtgag
      121 cttctccagg tcccccttga gccgctcccc ctgatagctg aaggccagct ccgccccggc
      181 gtcccggagc ctctcggcga tggcccatcc cagactgcgc gcgttggaga cccccatcac
      241 cagggcccgc tttcccttca gatcgatctg gatcatggcc gggtatctta tacgacggag
      301 gtggaccgag catgagcacg acccgaaccc ttggaatcct gctggtggca ctggccgccg
      361 gcgcctgcac acccccgtcc cccggtcccg ccaccggcgg cgacgggtcc acggagagcg
      421 cccaggccgg cccgggcccg gcccggatcg gcgccccccg gacggacgcc cccgagacga
      481 gagccggcgc cgatctgcgg ggcacgatca cgcagacctc ggccgtggag cccggcggcc
      541 ccggcgctcc ggctctcggc cgcgtgctcg tcgaggggga ggtcgagccc gacacccgct
      601 atgaccgggg ctggatcacg gtgaccgaag agacccgcat cgtgcgaagg gaggggatga
      661 gcgaccgccc cccctccttc ctggacctga cggtggggct ccgggtggag gtcaccttcg
      721 agggggcggt ggccaagtcc ctccccctcc aggccaccgc gcgggaaatc gtgatcctcg
      781 gcgccgcgcc ggcgcccggt ccgggagagg aggacggcaa gacggcaggc ggcgaggcgg
      841 ccgacttcaa ggggacggcc gggatcatcg acaagcctcg ccccgacctc ccgcccgcca
      901 ccctgcgcga cgtccgtacc gcccggcagg cgggcttcga ccgggtcgtc ttcgaattcg
      961 cgggaggggc gctgcccggc tatcacctgg agtacatcga caagcccgtg cgccagtgcg
     1021 gctcggggga gccggtcccc ctggccggcg acgcctggct gcaggtccgg ctcgtcccgg
     1081 cctatgccca taccgacgcc ggacaggcga cggtccagga acgggagcgc cacctggact
     1141 accccgtcct gcgggagctg gagtccacct gcgacttcga ggcgcacgtc gagtgggtgc
     1201 tgggcgtcgc ctcccccaac cgctaccggg tcctggagct ctcggacccc acccggctgg
     1261 tagtggacgt ccggcactga cctctagaga ccgccgagtt gcgcgaggag cgcctccaga
     1321 tcccgctgac tgacatccgc gtccgggaag cgcgcgggat cgtagacgct cccctcggca
     1381 ggcggccgcg gagtttcagc tgccggggcc gccggcgcgg cggtctgggc cgctggcggc
     1441 tcctcggccg cgacggctcc caggtcggcg gccaggccgg cgatggtcgg gcgctcgaag
     1501 agccggccgg gggtgagggc cagcccccgc tcccgcgccc gggcgacgat ccgcaggctg
     1561 agcaccgagt cgcctcccag ctcgaagaag tcgtcgtgga tgcccacggc gtcgatcccc
     1621 agcaggtcgc cccagatctc cgccagccag ctctcggcct cggttcgtgg ggccacgtag
     1681 gcggtggcga ggtcgggccg gccgtgccgc ggcccggccg gcgcggccgc gccggcgcgg
     1741 tccagctcgg ccaggatgcg ctcacccgtc aaggccgcgg cttcggcaag gcgcgccgtg
     1801 aggtcccgca ccgagaccat cacctggggg aggtcccccc cactcagaat gcggcggaag
     1861 gcttccactc cgtcggccga ggtgagcccc tcgccagcgg ccacgggaac cggagccggg
     1921 ggcgcgcttt cgaccatctc cagccgggac gggacgttgg gacgcgcggc gacccgccgg
     1981 aaggcgaagt cgtcgatgcg caccaggacc tcgcccgcgg gatcgaagag caccacctcg
     2041 aaatgcagct cctcgccgct gccttgcttg gtgcggatga agctgaagag ctcggccggc
     2101 agcggccgtt cgatccgcag gccggcgtac gagaacggca tgtagacgcc gtcggccgcg
     2161 gggccgagcc actgggagaa gccggtggcg cggtcgagga ccgccgggtg gagccccagc
     2221 gcttcgagct cggccgcgaa ctcgcccggg aaccgcagct ccgcgaggaa gcggccgtcc
     2281 gccgccgcgg tccggtgcag gctgcgccag cgcgggccga aggacatgac ctccgagctc
     2341 ccgaggtcga cggccgccgg ctcgaccggc agctcggcga gcagctcccg cagggacgga
     2401 acgggcgcgc cagagcccgc cggcggaccg atctcgccca gcgcgtgctc ctgccaccgc
     2461 gtctcgccgc cggcctccag ccggctggcg acgcggaagc tccaggcgtc gccctggcgg
     2521 cgcagcagcg tgtagacgtc gcggctctcg ccgtcgggca cggcgagcgg ggtgacgaag
     2581 gcggcctggc ggatctcggc cgcggcgctg ccggtgctct gctcgaaggc ggcgcgcgcc
     2641 agctccagat aggcggtgcc cggcagcacc ggcgtcccct ggatgcggtg ctcgtccagc
     2701 acccagtggc gcgccgcgtc gagacgggtg acgaaggcgt gctcgccggc gccggccggg
     2761 acgcgccggt cgagcagcgg gtggtccacc gcggcgccga ccggcaggcc gtccatcagg
     2821 cgccgcgccc ccggcgccgc cgtctccacc gccatgccgg cctcggtcca ggcgtcccag
     2881 gcgatggaaa cggtgagccc ggcgctgccg gcgcgggagc cggcgaaggc gtcgaggaaa
     2941 gcgttggcgg cacagtagtc cacctgcccg taggcgccga tcaccgagtt gagcgacgag
     3001 cagagcacga aaaggtccag ctcctcggga gggaaagtgg cggcgagggt cagggtgccc
     3061 tggaccttgg gggcgagcac aacgtcggcc gcgatccggt ccttgcgcgc gatcacgccg
     3121 ccgccgggga tgccggcggc gtggatcacg ccgtgcaccg cgccccaccg ctcgagcgcc
     3181 agggcgcgga cctggaccat ccccacggga tcggtgacgt cggcctggac cgccaggacc
     3241 tcggccccgg cctcctccag gtcccgcagg cgccggagct tgcggaagag cgggccgtcg
     3301 ccgccgcggg cgagcaggcc gtcccactcc tcgcgcgggg gcagcgcggt gcggccgatc
     3361 aggaccagcc tggcgccggc gctccgcgcc agccagcgcg cgatggcgtg gccgacgccg
     3421 ccgagcccgc cggtgatcac atagacaccg ccggggcgca gccgcggcgc cgccgggccg
     3481 ccctcctcca ggcgtacggg ctcgaagctc tgcagccagc gctgccggcc gcgccaggcg
     3541 atcaccggct ccggcgagcc ggccgccacc tcggcccaga ggcggtcgac ggtccgctcg
     3601 agccggccgc ccgcctccag atcgaggctg tggcagacga ccccggggag ctcgagcggg
     3661 atcaccttgc ccggcccgag cagcagcgcc cgctcgggga cgacggaatc gccgcccgag
     3721 acgtcgtgca ggtggctgga caccagccag aggtcgagcc gccgctcgtc gattcgccgg
     3781 cgctccagcg cctgcgccag gaagagcggg ctgtagaagg cgcggtcggc ggccgagccg
     3841 gccggcgcgg caccgggagc cggctgcgga gcgccgaagc tccacaggtg gaggatggcc
     3901 ccgacctccc tcccctcgga cggcaaggcg gcgagcaggg cgtcgtaatc cgccgcctct
     3961 cccgggcgca cggcgtaggc accgccgccg aggtcggcga agccgccgcc cggctggacg
     4021 gtggccacgc tccgcccggc ggcgcgcagc cgcgcggcga tcccttcgcc cagcccctcc
     4081 ccatccagga agacgaggac gacgccgtcc accccctcgc cggccggcgc cgccggcgtg
     4141 ggcg
//
LOCUS       DASPJI010000019         3022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_287886, whole genome shotgun sequence.
ACCESSION   DASPJI010000019 DASPJI010000000
VERSION     DASPJI010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287886"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VEL74_00515"
     CDS             complement(<1..385)
                     /locus_tag="VEL74_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HYG61035.1"
                     /translation="MKDRELKTPKKLIMGARRAAAAPAEELVREELPPSSSGAGLPLI
                     LRPVEPEFDGLSWVESQRGRLEELLQRHGALLFQGFGLSEPEDLQGFVRAICGDLLEY
                     RERSSPRHEVGDRIYTSTDYPAEERI"
     gene            complement(809..>3022)
                     /locus_tag="VEL74_00520"
     CDS             complement(809..>3022)
                     /locus_tag="VEL74_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61036.1"
                     /translation="ERDLSRSPLFQVLLNLQAFPHRSYDLPGGLSMTALPLQELPSRF
                     DLTLYGELVEGEGITLDLVYNADLFDRATMRQLLGQLHHLLERAVAEPELPVSGISLI
                     LPESAAVLPDPRLPLAGAAQGWHGAVHERFLEQARRDPGRLAVVDAGSAWTYGDLAQA
                     STALAVWLRAEGIECGDVVAIHAHRSAPLVQALLGVLMAGAAFLVLDPAHPAARRRSA
                     VLQARPRAWIEIEAAEPLPAELAAMADAVPRLQLDLSGEIYGVDEAELALPAVSPGDL
                     AYVAFTSGSTGEPKGILGTHGPLSHFCAWHARQFGLTGEDRFSVLSGLSHDPLLRDLF
                     APLGLGATLCMPDVDDLGSPARLAGWMARQRITVCHLTPAREQILAEGGVDLPRLRLA
                     FFGGDVLTERDVARLRDMAPAAACVNFYGTTETPQAVSWFDASGAGDWPGRRVPIGRG
                     IDDVQLLVLNEADDQAAPGELGEVCVRTPHLSLGYLGDGRLTAERFGIHPWTDGPEDR
                     LYRTGDLGRYRADGAVVLAGRRDGQVQVRGFRVEPAEVETALALHPAVREAAVLLREV
                     SGADGKGRELLTAWLVGEEGASRPAAGELREILRQRLPDPMVPEAFVWLERLPLTPNG
                     KLDRRVLPAPLPTAIEAVRADEPPADPVEEWVAELWQELLGLERVGRHDNFFDLGGHS
                     IVATRVVSRLEQDLGVEIPLRSLFETPTVAGLANRIVAVGMTAVSEAAEAEPVES"
BASE COUNT          486 a         1066 c         1085 g          385 t
ORIGIN      
        1 agatccgctc ctccgccggg tagtcggtgg aggtgtagat gcggtcgccc acctcgtggc
       61 gcggcgagga gcgctcccgg tactccagca agtcgccgca gatggcgcgg acgaaaccct
      121 ggaggtcctc cggctcgctt aggccgaagc cctggaaaag gagggctcca tgccgctgca
      181 ggagctcctc gagacggccg cgctgggact ccacccagga gaggccgtcg aactccggct
      241 ctaccggacg caggatcagg ggcagccccg cgccgctgct ggagggcggc aactcctcac
      301 ggaccagctc ttcggcgggg gccgcggcgg cccggcgggc tcccatgatc agcttcttgg
      361 gagttttcag ctctcggtct ttcatgtcgg ctccaggacg tgctgctcgc gggatcaaga
      421 cgcgggatcg aaaaggcggc ggcccaacag ctccggatac gcatggtagc agagggggcc
      481 agtacgccgt cattcccagg gaaatgaggt ccatccctcc cggggcaacc attgccccgg
      541 gagaaatggc ggtcattcct catggggcag tcattgcccc atcaggaacg gcctccattc
      601 ctggtggggc agcgactgcc ccgacaggaa tgatcgccat tcctgatggg gcagcgatcg
      661 ccccaacagg aatgatggcc attcctcgtg gggcaacgat ggccccggca ggaaggatga
      721 ccattcctcc aggggcagcg gctgccccat caggaatgat ccccgcccgc cgctgccgcg
      781 gcgactcctg acccgaagcg atgcgatgtc acgactcgac cggctccgcc tccgctgcct
      841 ccgacaccgc ggtcatcccg acggcgacga tccggttcgc cagcccggcg acggtggggg
      901 tctcgaacag ggagcgcagg gggatctcca cccccagatc ctgctccagg cgcgacacca
      961 cgcgggtcgc gacgatggag tggccgccga gatcgaagaa gttgtcgtgc cggcccactc
     1021 tctcgagccc cagcagctcc tgccagagct cggccaccca ctcctccacc ggatcggctg
     1081 gaggctcatc ggcccggacg gcctcgatgg ccgtggggag cggcgcgggg agcacccggc
     1141 ggtccagctt gccgttgggg gtgagcggga gccgctccag ccagacgaac gcctccggca
     1201 ccatggggtc cggcagccgc tggcgcagga tctcccgcag ctcgccggcc gcgggccggg
     1261 aggcgccctc ttcccccacc agccaggcgg tgagcaactc gcgtcctttt ccatcggcgc
     1321 cggagacctc gcgcagcagc accgcggcct cgcgcacggc gggatggagg gcgagggccg
     1381 tctccacctc ggccggctcg acgcggaacc cgcgcacctg cacctggccg tcgcggcggc
     1441 ccgccagcac cacggcgcca tccgcccggt agcggcccag gtcgccggtg cggtagaggc
     1501 ggtcctcggg gccgtccgtc cacgggtgga tcccgaagcg ctccgcggtg agcctcccgt
     1561 cgccgagata gccgagcgag aggtggggag tgcggacaca gacctctccc agctcccccg
     1621 gcgcggcctg gtcgtccgcc tcgttgagca ccaggagctg gacgtcgtcg atcccccggc
     1681 cgatgggcac gcggcgcccc ggccagtctc cggcgccgga ggcatcgaac caggagacgg
     1741 cctggggcgt ctcggtcgtg ccgtagaagt tgacgcaggc ggcagccggg gccatgtcgc
     1801 gcaagcgggc cacgtcccgc tcggtcagga catcgccacc gaagaacgcg agccgcagcc
     1861 ggggcagatc cactccgccc tctgccagga tctgttcgcg ggccggggtg agatggcaga
     1921 cggtgatccg ttggcgcgcc atccagccgg ccaggcgggc gggtgagccc aggtcgtcga
     1981 catccggcat gcagagagtg gccccgaggc cgaggggggc gaagaggtcc cgaagcaaag
     2041 ggtcgtgaga aaggcctgac aagacgctga agcggtcctc cccggtcagg ccgaactgcc
     2101 gggcgtgcca ggcgcagaag tgggagaggg ggccgtgggt ccccaggatc cccttgggct
     2161 caccggtgga gccggaggtg aaggcgacgt aggcgaggtc gccgggactc acggccggga
     2221 gggccagctc cgcctcgtcg acaccataga tctctccgga aaggtcgagt tgcagccggg
     2281 ggacggcgtc cgccatcgcg gcgagctcag ccggcaacgg ctccgcggcc tcgatctcga
     2341 tccaggcccg tggccgcgcc tggagcaccg cggaccgccg ccgcgccgcg gggtgggccg
     2401 ggtcgaggac cagaaacgcc gcgccggcca tcagcacgcc aaggagcgcc tgcaccaggg
     2461 gggcgctccg atgggcatgg atcgccacca cgtcgccgca ctcaatcccc tccgcccgca
     2521 gccagacggc gagggccgtg gaggcctgcg ccagatcgcc atacgtccac gcgctgccgg
     2581 cgtccaccac cgccaggcgt cccgggtcgc gccgggcctg ctccaggaag cgctcgtgca
     2641 ctgccccgtg ccacccctgc gcggcgcctg cgagggggag gcggggatcg ggaaggaccg
     2701 ccgcgctctc gggaaggatc agagagatcc ccgacaccgg cagctcgggc tccgccaccg
     2761 cccgctccag gagatggtga agctgcccca ggagctggcg catggtggca cgatcgaaga
     2821 ggtcggcgtt gtagaccagg tccagggtga tcccctcgcc ctccaccagc tctccataga
     2881 gcgtcagatc gaagcgggac ggcagctcct gcagcggcag agccgtcatc gacagcccgc
     2941 ccggcaggtc gtaggagcgg tgggggaagg cctgcaggtt gagcagcacc tggaacaggg
     3001 gggaacggga aaggtcgcgc tc
//
LOCUS       DASPJI010000020         4572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_288104, whole genome shotgun sequence.
ACCESSION   DASPJI010000020 DASPJI010000000
VERSION     DASPJI010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288104"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VEL74_00525"
     CDS             <1..577
                     /locus_tag="VEL74_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020562103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61037.1"
                     /translation="RDTGESALVSRSFQSPAEAGSSPAHRWVTGALSADGGTVAFTST
                     ATNLVDGDRNCLADAFLYDNGRIGHFYTLPPCRFLDTRLPQDGPALASGQTALLHLYN
                     TCGIPETAKAVAVNVTAVGATGQGHVAFYRGDAVAAPGTSTLSFRPGLTRASFAILPL
                     AANADGSLKVVPSVTGGGTVHVLIDVAGYFE"
     gene            complement(585..1181)
                     /locus_tag="VEL74_00530"
     CDS             complement(585..1181)
                     /locus_tag="VEL74_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG61038.1"
                     /translation="MEPFEIPTLETERLRLRPFRPDDIDDYAALYANPEVVRYLGAGT
                     EPWDRGRSWRHMAFLVGHWRLKGAGIWVIEQKETGTFVGTGGFAEPDGWPGFELAGCL
                     APHCWRKGFAFEAGRAALDYAFDVLDKDRVISLVHPENRASIRVVERLGQSLQGRIDH
                     FGREMLVFGIDREIHARRQDRVEVLPEPREGREMPGPL"
     gene            1322..1549
                     /locus_tag="VEL74_00535"
     CDS             1322..1549
                     /locus_tag="VEL74_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61039.1"
                     /translation="MLRLVLLVLCISSSLPTLIQLKGGGGWDPNGLKSGTPPVILPGE
                     GGGGNDPDGLTAQPPPSTTGGEIGGGWDPNG"
     gene            1676..3001
                     /locus_tag="VEL74_00540"
     CDS             1676..3001
                     /locus_tag="VEL74_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61040.1"
                     /translation="MSHLGREVLRRVLAGEGTAPEADRAEEHLVSCETCRAQAATLLD
                     ELRVARPELRGEDSLRLVFDLIDRERRRGVEDLAALAEWAELRRISSRRSQRDRVRMT
                     KACHTIAFFNLLLRELKEAAAWEEAEFVASLAVLSCERMSQRQQVSEPSKNDLLAEVW
                     TAVANSRRRAAEWGKAHQALTFAERYLKSGTGDPRLEASLLSIGASTLADEGHVREAV
                     EALDRCGQIYQGLTEWPLLARTLIQKANILAGTEPVQGLSALDHALPFIPAGDPRLLL
                     FAEMLRVECLIEVRKPVEALQVFRRSSHLLDETPQIRPRIRGRFTSARLLDALGFQQH
                     AERLFDEVVARDIEHELYKDALLDLLYLYGRHVKTGEFEKAAGVCTRALTDASLAEIT
                     HDQIRDLWTQLLNAAQVHAISQDVLKDLRRYLNVHWKQPAAQPPVVRLR"
     gene            complement(3091..>4572)
                     /locus_tag="VEL74_00545"
     CDS             complement(3091..>4572)
                     /locus_tag="VEL74_00545"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HYG61041.1"
                     /translation="ILLSAPETQKAKLPAEKELLGTFQQVTARQINPWVDRVRQGPLV
                     AEAPKPGRIVSESRVEELNVTEWRLSNGVRVIMKPTDFQNDEILLTSYSPGGSSLVPD
                     SKYLSAAFATAVIRDAGLGRFDRITLEKALAGKAAGVLPYIGTFEEGIRGVAAARDLE
                     TMFQLVYLNVTAPRKDPKLFLSLLGGLEEQLRTRVSSPEAVFADELRKAMAQGNPRQE
                     PLSLERLQEIDFETAYQVYRDRFADVGDFTFVLVGNFTPESIKPLVLTWLGSLPTKGR
                     RESWRDPGDSTTFDSVVKVDVAKGLEPKSQVRMLFSGPATFSRENRHVMESLARVLQI
                     RLREVLREDMGAVYGVQAEGGLSSRPRGEYTFHIAFGCAPEKVDSLVQAVFAEIESIQ
                     KNGVPDGYLQKVREQERREREVAERENRFWLGALEAYASEGWDLRGILRYGELVEMVT
                     SERLQQAAQAYFHRDRYLLGVLRPEAPVPAQTAPAAVSTTTGR"
BASE COUNT          686 a         1604 c         1537 g          745 t
ORIGIN      
        1 ccgcgacacg ggggagagcg cgctggtctc ccgttcgttc cagtcgccgg ccgaggccgg
       61 gagctccccc gcgcaccgct gggtgaccgg agcgctgagc gccgacggcg gcaccgtggc
      121 gttcacctcc accgccacca acctggtgga cggcgaccgg aactgcctgg ccgacgcctt
      181 cctctatgac aatggccgca tcgggcactt ctacaccctc cccccctgtc ggttcctgga
      241 cacccggctg ccgcaggacg gtccggcgct ggcctcgggc cagacggcgc tgctccacct
      301 ctacaacacc tgcggcatcc cggagacggc gaaggcggtg gcggtcaacg tcacggctgt
      361 cggcgccacg ggccagggcc acgtcgcctt ctaccgcggc gacgccgtcg ccgcgccggg
      421 gacgagcacc ctcagcttcc gtcccggcct gacccgcgcc agcttcgcca tcctccccct
      481 ggccgccaac gccgacgggt ctctcaaggt ggtgccgtcg gtcaccggcg gcggcacggt
      541 gcacgtactg atcgacgtcg ccggctactt cgaataggcc tggatcagag agggcccggc
      601 atctcccggc cctctcgcgg ctctggcagg acttctactc ggtcctggcg gcgggcgtgg
      661 atctcccggt cgatgccaaa gacgagcatc tcccggccga agtggtcgat gcggccttgc
      721 agactctgtc caagccgttc gacgacgcgg atggaggcgc ggttttccgg gtggacgagg
      781 ctgatgaccc ggtctttatc cagaacatcg aaggcgtagt ccagggcggc ccttccggct
      841 tcgaaggcaa accctttcct ccagcaatgg ggcgccaggc atccggccag ctcgaagccc
      901 ggccagccat ccggctcggc gaagccaccg gtccccacga acgtgccggt ctccttctgt
      961 tcgatcaccc agatgccggc ccccttgagc cgccagtgcc ccaccaggaa agccatgtgg
     1021 cgccaggagc ggccccggtc ccacggctcc gtccccgcgc ccaggtagcg caccacttcg
     1081 gggttcgcgt acagcgccgc gtagtcgtcg atgtcgtccg gccggaaggg ccgcaggcgc
     1141 aggcgctccg tctccagggt gggaatttcg aacggttcca tgcttcgatt cctttctccg
     1201 ccgtgatcgt tcgtggagac gaagcttgtc cttcggaccg gaggggcgga cactccggcc
     1261 gtggggacgc ctccacgggc aatggcgccc gtgcggtcac ctgtccccgg agggagaagc
     1321 tatgcttcgc ctcgttcttc tcgtcctctg tatttcctcg tcactcccaa cgctgatcca
     1381 gctcaaaggt ggcggcggct gggaccccaa cggattgaag tccggtacgc cgccggtcat
     1441 cctgccgggc gagggcggcg gcggcaatga ccctgatggg ctgacggccc agccgccgcc
     1501 ttccaccact gggggagaga tcggcggggg ttgggacccc aacggctgat cccgccggcg
     1561 cgaggggcgt gctcggcgcc cctcgctttt ctctcctacg cgaactgtac gtagccttgc
     1621 actttgctgg gagctaaagt accctgagct ggaacctatt tgaggaggat cttgaatgtc
     1681 ccacctggga cgggaggtgt tgcggcgagt tctggccggc gaaggaaccg ccccggaggc
     1741 ggaccgggcc gaggagcacc tcgtctcctg tgaaacgtgc cgggcccagg ccgcaaccct
     1801 gctggacgag cttcgcgtgg cgaggcccga gttgcgaggg gaggactctc tgcggctcgt
     1861 cttcgatttg atcgaccggg aacggcggcg tggggtcgag gatctggcgg cgctcgccga
     1921 gtgggccgag ctgcgccgca tctctagccg caggagccag agggaccggg taaggatgac
     1981 gaaggcgtgc cacaccatcg ccttcttcaa cctcctgctc cgtgagctca aggaggccgc
     2041 tgcctgggag gaagccgagt tcgtggcctc cctcgcggtg ctgagctgcg aacgcatgag
     2101 ccagcggcag caggtctccg aaccctcgaa gaacgacctg ctggcggagg tctggacagc
     2161 ggtcgccaac tcccggcggc gggcggccga atggggtaag gcgcatcagg ccctgacgtt
     2221 cgcggaacgg tacctcaaga gcggcacggg agacccccgt cttgaagcct cgctcctctc
     2281 gatcggcgcg tcgaccctgg ccgatgaggg gcatgtccgc gaggccgtgg aggccctgga
     2341 ccgctgtggt caaatctacc agggcctcac cgaatggccc ctcctcgccc gtacgctgat
     2401 ccagaaggcc aacatcctgg ccggtaccga gcctgtccaa ggcttgtcgg ctctcgacca
     2461 cgcccttccc ttcatccccg ctggagatcc acgtctcctc ctgtttgccg agatgctcag
     2521 agtagagtgc ctcatcgagg tccgtaagcc cgtggaagct ctccaggtct tccggcggtc
     2581 gtcacatttg ctggacgaga cgccccagat ccggccgcgc atccgtggac ggtttacgag
     2641 cgcccgtctc ctcgatgccc ttggcttcca gcagcacgcg gagcggctgt ttgacgaggt
     2701 cgtggccagg gacatcgagc acgagctcta caaggacgct ctcctggacc tcctctatct
     2761 ctatggacgt catgtgaaaa ccggcgaatt cgagaaggcg gcgggcgtgt gtacgagagc
     2821 cctgaccgac gccagcctcg ccgagatcac gcacgatcag atccgggacc tctggaccca
     2881 gctcctgaac gccgcccagg tccacgccat cagccaggac gtgctcaagg acctccgccg
     2941 gtacctgaac gtccactgga aacagcccgc cgcccagccg ccggtggtga ggctgcggta
     3001 gaggcggcga cagcgccgaa gacctccacg gccacgccga tgaagggccc ggcgcagcgc
     3061 cgggcccttc ctgttttgct ggatggatgt ctagcggccg gtggtggtgg agaccgccgc
     3121 cggcgccgtc tgcgccggga ccggcgcctc gggccgcagg acgccgagca ggtagcggtc
     3181 gcggtggaag taggcctggg cggcctgctg cagccgctcc gacgtcacca tctccaccag
     3241 ctcgccgtag cgcaggatgc cgcgcaggtc ccacccctcg ctggcgtagg cctccagggc
     3301 ccccagccag aagcggttct cccgctcggc gacctcgcgc tcccggcgct cctgctcgcg
     3361 caccttctgc aggtagccgt ccgggacgcc gttcttctgg atcgactcga tctcggcgaa
     3421 caccgcctgc accagggagt ccaccttctc cggcgcacag ccgaaggcga tgtggaaggt
     3481 gtactcgccg cgcggacggc tcgacagccc gccctcggcc tggacgccgt agaccgcgcc
     3541 catgtcctcg cgcagcacct cgcgcagccg gatctggagc acccgcgcca gcgactccat
     3601 gacgtgccgg ttctcgcggc tgaacgtcgc cgggccggaa aacagcatgc gcacctggct
     3661 cttcggctcg agccccttgg ccacgtccac cttcaccacg ctgtcgaacg tcgtgctgtc
     3721 gcccgggtcg cgccagctct cgcgccgccc cttggtgggg aggctgccga gccaggtgag
     3781 gaccagcggc ttgatgctct cgggggtgaa gttgcccacc aggacgaagg tgaagtcgcc
     3841 gacgtcggcg aagcggtcgc ggtagacctg gtaggcggtc tcgaagtcga tctcctgcag
     3901 ccgctcgagg ctgagcggct cctggcgcgg gttgccctgc gccatggctt tccgcagctc
     3961 gtccgcgaag accgcctcgg ggctggagac gcgggtgcgg agctgctcct cgaggccgcc
     4021 gagcagcgac aggaacagct tcgggtcctt gcgcggcgcc gtgacgttga ggtagacgag
     4081 ctggaacatg gtctccaggt cgcgggccgc ggcgacgccg cggatcccct cctcgaacgt
     4141 gccgatgtag ggcagcacgc ccgcggcctt gccggccagc gccttctcca gcgtgatgcg
     4201 atcgaagcgg ccgaggccgg cgtcgcggat cacggcggtg gcgaaggcgg ccgacaggta
     4261 cttgctgtcg ggcaccaggg agctgccgcc cgggctgtag gaggtgagga ggatctcgtc
     4321 gttctggaag tcggtgggct tcatgatcac ccgcacgccg ttcgacaggc gccactcggt
     4381 gacgttcagc tcctcgacgc ggctctcgga gacgatgcgg cccggcttcg gcgcctcggc
     4441 caccaacggc ccctggcgca cgcggtccac ccaggggttg atctggcgcg ccgtcacctg
     4501 ctggaaggtg ccgagcagct ccttctcggc gggcagcttg gctttctggg tctcgggggc
     4561 ggagagcagg at
//
LOCUS       DASPJI010000021         3186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_459980, whole genome shotgun sequence.
ACCESSION   DASPJI010000021 DASPJI010000000
VERSION     DASPJI010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459980"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="VEL74_00550"
     CDS             <1..1329
                     /locus_tag="VEL74_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYG61042.1"
                     /translation="PNSVLAPEVETFPEAFRRQGFRTAGFTEGGYMSGRFGFRRGFEQ
                     FAARARNRNRPLHQTFRRGVRFLEGLKPDERFLLFLHTYSVHTPYDAPEEYRKPFWPG
                     EPPAGAIPATGPAFGQHNASGGTLPEPVVGWLTALYDAGISQTDEVLRGFFADLERLG
                     LADDVTVIVTSDHGEELQDHGRLNHEQLYRETMRVPLLVIHPDQRTAVRHGGVVQLVD
                     LAPTLYELARVQPKGKPTGTSLAGLLGSPAAPRPGSAWMEGFGARAVYRGEQRRLDSL
                     LLFDQPEGTWVSRSVAVDTPGGRELSFQARSYQEPRRLTVRQGDAVLAEVALTPEWTP
                     VRLPLPNPGRLRLEADGCAVAAPEREHDKWDVRCQAFQVRGLRLSHVEVYDVSQDPGQ
                     RRDLSRERTRETRMLLRDLLAFNPKPVAEATTQELDPELEKNLRALGYLQ"
     gene            complement(1333..2709)
                     /locus_tag="VEL74_00555"
     CDS             complement(1333..2709)
                     /locus_tag="VEL74_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61043.1"
                     /translation="MTDHQAALRVRAFFVLGLLISIWMVARSQVGGDQLNLLARGWIL
                     ADEGQWISYGNPMSTGGKAPGGITSLLVGLPLMVWRDHRAPTVVVLLFHILAYWLLDR
                     TLRPILSPRERVFLSVLYWLNPWRLYFSAFLWNPNYLFLLSAIHLWSCLTQRDRARFW
                     PSFLHGLALLLALQIHASFLLLAAATGLLWLRGWFRVHWPGMILGAVLAALPLVPWVL
                     DLREDPAIITEADKGFLGRGLIYLFPLLRGLLYWLRYSSLAVSEKVVNFDFGDFMDPE
                     TGASLGKAVGSVAEVLLSLTLILPLLANRWLWKRFRRRIRRRFPEGGSDRAWLQGYAA
                     WSFVGAVAVFSLSPTTIMMWQALILLHAAILPLILWAGILVRTPRAPRVLRIERVYIA
                     ATMTLLALIPLGSTHYRCVGHDGGRDSYGLPLRSDHPMLHELNIQSTCPLPLNRPDGW
                     WPDVLPER"
     gene            complement(2706..>3186)
                     /locus_tag="VEL74_00560"
     CDS             complement(2706..>3186)
                     /locus_tag="VEL74_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HYG61044.1"
                     /translation="GAADPIRYYEFELSPQGVLFDATIHNPTSHRADMLTDPTWDCPN
                     ILWAVGRGGLGRVRPAQATGSVGGWEGGAHTKEPSTDGPAQDRGANASYQDWWAALAI
                     PWAAIAPVSTPLPPIWRANFYRIERPRDGVPEFSCWSPTLTDPADFHKPARFGALEL"
BASE COUNT          507 a         1082 c         1190 g          407 t
ORIGIN      
        1 ccgaactcgg tgctggcgcc ggaggtcgag acgttcccgg aggcgttccg gcgccagggg
       61 ttccgcaccg ccgggttcac cgagggggga tacatgagcg gccgcttcgg cttccggcgc
      121 ggcttcgagc agttcgccgc ccgcgcccgc aaccgcaatc ggcccctcca ccagaccttc
      181 cggcgcggcg tgcggttcct cgaagggctg aagccggacg agcgcttcct cctcttcctt
      241 catacctact ccgtccacac cccctacgac gcgccggagg agtaccggaa gccgttctgg
      301 cccggcgagc cgccggcggg ggcgatcccg gcgaccggcc cggccttcgg ccagcacaac
      361 gcgagcggcg ggacgctccc cgagcccgtg gtcggctggc tcacggcgct gtacgacgcc
      421 ggcatcagcc agaccgacga ggtgctgcgc ggcttcttcg ccgacctgga gcggctcggt
      481 ctcgcggacg acgtgacggt catcgtgacc tcggaccacg gcgaggagct ccaggaccac
      541 ggccggctca accacgagca gctctaccgc gagacgatgc gcgtgccgct gctggtgatc
      601 catccggacc agcggacggc ggtgcggcac ggcggcgtgg tgcaactcgt cgacctcgcc
      661 ccgacgctct acgagctggc gcgggtccaa ccaaagggga agccgacggg gacgagcctc
      721 gccggcctcc tcggcagccc ggcggcgccg cgcccgggga gcgcctggat ggagggcttc
      781 ggcgcgcggg ccgtctatcg cggcgagcag cgccggctcg acagcctgct cctcttcgac
      841 cagcccgaag ggacgtgggt ctcccgcagc gtggccgtgg acacccccgg cggccgggag
      901 ctgtcgttcc aggcccgctc ctaccaggag ccgcgccggc tgacggtgcg gcagggggac
      961 gcggtgctcg ccgaggtggc gctcaccccc gagtggacgc cggtccgcct ccccttgccg
     1021 aatcccggcc gcctgcgcct ggaggccgac ggctgcgcgg tggctgcccc ggagcgcgag
     1081 cacgacaagt gggacgtccg ctgccaggcc ttccaggtgc gcgggctccg cctctcccac
     1141 gtcgaggtct acgacgtctc ccaggacccg gggcagcgcc gggacctctc ccgcgagcgg
     1201 acccgggaga cgcggatgct cctgcgcgac ctgctggcgt tcaatcccaa accggtcgcc
     1261 gaggccacga cgcaggagct cgaccccgag ctggagaaga acctgcgggc gctcgggtat
     1321 ctgcagtagg cgtcagcgtt ccggcagcac gtccggccac cagccgtccg gccggttgag
     1381 cggcaggggg caggtggact ggatgttcag ctcgtgcagc atggggtggt cggagcgcag
     1441 ggggaggccg tagctgtccc ggccgccatc gtgcccgacg cagcgatagt gggtgctgcc
     1501 cagcgggatg agcgccagca gggtcatcgt cgccgcgatg tagaccctct cgatccgcag
     1561 cacccgcggc gcgcggggcg tccggaccag gatgcctgcc cagaggatca aggggaggat
     1621 ggcggcgtgc agcaggatca gcgcctgcca catcatgatg gtggtcggag agagcgagaa
     1681 gaccgccacc gctcccacga agctccaggc cgcgtaccct tgcagccagg cacggtccga
     1741 gcccccctcc gggaagcgac gccggatgcg gcggcggaaa cgcttccaga gccagcggtt
     1801 ggccagcagg gggaggatca gggtgagcga caggagcacc tcggccacgc tccccaccgc
     1861 cttgccgagg ctcgcgcccg tctccgggtc catgaagtcc ccgaagtcga agttcacgac
     1921 cttctccgag acggcgaggg atgagtagcg cagccagtag aggaggccgc ggagcagagg
     1981 gaagagatag atcaggccgc ggcccaggaa gcccttgtcc gcctcggtga tgatggcggg
     2041 gtcctcccgc aggtccagca cccaggggac caggggcagc gcggccagca ccgcgcctag
     2101 gatcatcccc ggccagtgca cccggaacca gccgcgcagc cagaggaggc cggtggccgc
     2161 cgccagcaat agaaaggacg cgtggatctg gagcgccagc agcagggcga ggccgtgcag
     2221 gaacgacggc cagaagcgcg cccggtcccg ctgggtcagg cagctccaca ggtggatggc
     2281 gctcaggaga aagagatagt tcgggttcca gaggaacgcc gagaagtaga gccgccaggg
     2341 gttcagccag tagaggaccg agaggaagac ccgctcgcgt ggcgacagga tggggcggag
     2401 cgtccggtcc agcagccagt aggccaggat gtggaagagg aggaccacca ccgtgggcgc
     2461 acggtggtcc ctccagacca tcagggggag gccgacgagg aggctggtga tcccccccgg
     2521 cgccttgccg ccggtggaca tcgggttgcc gtaggagatc cactggccct cgtcggccag
     2581 gatccagccg cgggccagca ggttgagctg gtcgcccccc acctggctgc gcgccaccat
     2641 ccagatggag atcagcaggc cgaggacgaa gaaggcccgg acgcggaggg cagcctggtg
     2701 gtcggtcaaa gctcgagggc tccgaagcgg gcgggcttgt ggaagtcggc cgggtcggtg
     2761 agcgtcggcg accagcagga aaactccggg acgccgtcgc gggggcgctc gatgcggtag
     2821 aagttggccc tccagatagg ggggagaggg gtggaaacag gtgcgatggc ggcccagggg
     2881 atggccaggg cggcccacca gtcttggtag gaggcgttgg ccccccggtc ttgggcgggt
     2941 ccgtccgtgc tcggctcctt cgtgtgcgct ccgccctccc accctcccac gctgccagtc
     3001 gcctgcgctg gacggacccg cccaagcccc cccctcccga cggcccagag gatgttggga
     3061 cagtcccagg tggggtcggt gagcatgtcg gcgcggtggg aggtcgggtt gtggatggtg
     3121 gcgtcgaaaa ggacgccttg ggggctgagc tcgaattcgt agtagcggat cgggtccgcg
     3181 gcgccg
//
LOCUS       DASPJI010000022         9757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_344610, whole genome shotgun sequence.
ACCESSION   DASPJI010000022 DASPJI010000000
VERSION     DASPJI010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9757)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344610"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2782
                     /locus_tag="VEL74_00565"
     CDS             <1..2782
                     /locus_tag="VEL74_00565"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HYG61045.1"
                     /translation="GVYRSTDAGETWQRVGLEASERVARIAVHPKDGNVVWVCATGAL
                     WSSHPERGVYKTADGGKSWKRVLGVDADTGCSDLAVDPQDPRILYAGMWQFRRTPWSF
                     DSGGAGSGLYKSTDGGETWRELEKGLPAGEKGRIAVAVAPSRPSVVYTLVEAEKTALY
                     RSDDVGESWAEVNSSFTVSGRPFYFALVVVDPTDFDRVYKPGFTLGVSSDGGRSFGSP
                     FSSFENSVHSDHHALWIDPRNPQHLLLGTDGGLYISYDRGRRWRMVRGLPISQFYRVS
                     YDMAEPYNVYGGLQDNGSWRGPSRSVAGIQNRDWENIGMGDGFYAFADPADPEYAYVE
                     YQGGMISRLRLSTGERKEIRALPREGEEGLRFNWNTPIHLSPNENGTIYLGSQYLLRS
                     RDRGDSWERISPDLTTNDPRKQQQATSGGLTIDNSTAENHTTIYAIAESPKNGKVIWA
                     GTDDGNLQVTRDGGATWANVAANVPGVPRGTWVSGVEASPHDEATAFATFDVHATGDM
                     KTYVYRTTDFGRTWTALMPAAIPEAAGTGETLDGYAHVIRQDVVNPDLLFLGTETGLF
                     VSIDGGARWARFTGNLPRVAVRDLAIHPRDSDLIIATHGRGIYILDDITPLRALTREV
                     LDAEVALLPSRPSALAIPASIQDFAGDDEFVGANPPEAAAITYYLKKRHLFGDLKLEV
                     YDAQGAKIATLPGGKRRGINRVAWPMRLPAPKLPPANSLVANQFTFVGPRVPAGPYMV
                     KLVKGDQTLTGEVRLVPDPRSTHPEADRTLQQRTAMELYRRLEDLTYVVDAAVAARDG
                     ARQRAAALPAGDRLRRRLEALAGELETFRNGLVASGEGGMLSGEEQLREKLGDLYGAV
                     NGYEGRPTQSQIDYARVLDGELERAAARFEAIRASALSADGEIGRELARRKLEPIRVP
                     SREEWQKEREKG"
     gene            complement(2876..3085)
                     /gene="rpsU"
                     /locus_tag="VEL74_00570"
     CDS             complement(2876..3085)
                     /gene="rpsU"
                     /locus_tag="VEL74_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019952611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HYG61046.1"
                     /translation="MPVVHVREEESFENALRRFKRKVEKSGILTELRKRQHFEKPSVK
                     RKRKTIQAKKKIARKLMEERRQGLA"
     gene            complement(3181..4095)
                     /locus_tag="VEL74_00575"
     CDS             complement(3181..4095)
                     /locus_tag="VEL74_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61047.1"
                     /translation="MRPGFSPQRLGRRLGKTGLAALLALTLSPFGATGCGPDEEAKPA
                     PTALAADAEWRWLQDSRRSLEEKRAQLAQAEAAARSAAPQPATQPNAQAGPQAPQDDV
                     ARRRQEVDALSTELRRRLVDFINADPPVEGRPMTPRQQEALRMKSDEEILVAQDFIQR
                     GGDYRRAIEIYQAALSVDPGNPRLRQELEKARGMRYMSRERFSQVKEGMTQDEVRTLL
                     GQPNLHNVREYTERKVSAWFYPKGPEGAAAAVWFERSGSGPARVYERDFDAVPPAPAA
                     APAGRSPARPAPPARRPPASPGLTSDTE"
     gene            4367..6139
                     /locus_tag="VEL74_00580"
     CDS             4367..6139
                     /locus_tag="VEL74_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61048.1"
                     /translation="MEQLNREIGDTVQRAVSDLRREISARLRESHDEILRRLEDVSPP
                     LPGAYLAHDHFAPSLAEAEATAEARGEARGEARAGTAARRGAFGELRDSLAAIDRARS
                     QAEILAALLEQTGRFASRAALLLLRGSEVRGWGGHGFGDADAALRGLALDGTPGGPWE
                     RLAGGAGTQRLSAADCAALCSRIDSPLPTGGVLIPMLLRDRVAAILYADSLEGAGLQP
                     DALQVLTYTAALAIETLPFRERASTATLEPAPPAADEDEGTAAAAPPPVSEPAAAMPA
                     WAPEPEPAPEPEPASSFVGEPEAPAHYAEYAEIEAEPEIEAVTGTEPEAYQEAAAPWA
                     PAEPSYEAPAEDALVYAPAPAYEPEPEGDVTAAEEPAAAWGGGYAATAVAEPEPEVMV
                     EAEPEQSYTAELPRTDFAAQPAFAAAAPVAEPVAAPGPAASETVLLPRAGLRDSGVLE
                     PAREPAPVTPIRPVPPPPPRETDEAAAGGTSEVRPPTDVQGPGWAFATTRVPVSPSDE
                     TLHEEARRLARLLVSEIKLYNEEQVEEGRRNRDLYERLKEDIDRSRQMYEERVDSRIL
                     RSTDYFYQELVRILAAGDSRTLGI"
     gene            6246..8486
                     /locus_tag="VEL74_00585"
     CDS             6246..8486
                     /locus_tag="VEL74_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HYG61049.1"
                     /translation="MRSSRIRRSRRIPFSTVSLVLLLPLLLGSSPRTPLKPSVFPPVP
                     VPSFSDRVTLQYEQAASALRRNDCAGAAKALTPVMASKGSEAGFARLLSGLYAHSCEQ
                     VPLAEERLFGAADPDGVLEDWRLYLLSDAAMARGHVLLAQASLAKLLGDYPASPLRPR
                     ALLKAATLAWERGDSQRAQELIEQKRREDLQGEDAVALEKLAWEIANRLGDPRLQRDA
                     ARQLLIQAPTVASSVQAAEVFRRPDGQLEWSSFLTAQQIERRAETLLGMKLASNALGV
                     LDAVRPAERDAAWYLLKARALTAMNRGQEALALLSSVTGADPAQTAALYWQRAQAAEN
                     VGTAQRGRENLPAAERQRYRWMAQQNLEKVVEAGGDKDLSVKALRSLYADAQQDGSFE
                     RGLELLRRLRAVDPADTTGAANLWNLGWREYNQRNYTGAVGYWTELFALYPREHNGRR
                     GRYWTGRAFEALGETERANQIYAEVAGADTTDFYRKNALARLRRQPVTAGGAARERDV
                     EPWLADPVLSRARLLTDVGLDELAMSEAALVAGKAEPRAMRALEALVLGRKGDRRKSV
                     QVIRDAYPALGGAFQAGLPVQARQLYYPLDYQEQVRAWAGTNHLPAYLVFGIIRQESA
                     FDTNAQSWAGARGLMQLMPATARELAGKFGLPYSHEKLSEPAYNLRLGTSYFRQVLSM
                     FDGNVELALAGYNGGPYRMKRLWAESNGELDRFLESLTIEESKTYVKRILVLSDSYRQ
                     LYPHVG"
     gene            8500..>9757
                     /locus_tag="VEL74_00590"
     CDS             8500..>9757
                     /locus_tag="VEL74_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYG61050.1"
                     /translation="MPIATVCRRLATTVLPLLLLACGGGAPSPGTPAAGDSTGAGGPI
                     VLITFSSLRADVVGGLGGLPGLTPNLDALIRQADWSGRAIATTSRSEPALASLWTGLR
                     PWQHQVLHGGATLSPDLLTLAEALAAAGYSTAGFPGGPWYSQAEGFAQGFETFDPADR
                     GRGAGARLRGFGAGEARGRQFVWVHIPEPQAPYVLRRRLLPRVERLGGLPRELPMRIR
                     PLQLEPYFDPGVPLPEGKRRRFWAMYLLNVAWADEKLGRLIEALRGSGQWDRTLLAVA
                     AVHGEEFGERGQIGHGGNLGRRLLEVPLVVKLPKEDGGRRIAVPKGERVAAARLWATL
                     VEAAGGPAGHPTVPAAAPSLFRPAPWPALSELYLTNGANVFSLVEGDWQLLWESRFAP
                     AEPGYYRARYAALTGRQPDELFGRLRS"
BASE COUNT         1348 a         3548 c         3547 g         1314 t
ORIGIN      
        1 cggcgtctac cgcagcaccg acgccggcga gacctggcag agggtggggc tcgaagcgtc
       61 ggagcgcgtc gcccgcatcg ccgtccaccc gaaggacggc aacgtcgtct gggtctgcgc
      121 caccggcgcc ctctggagct cccacccgga gcggggggtc tacaaaaccg ccgacggcgg
      181 caagagctgg aagcgggtgc tgggcgtgga cgccgacacc ggctgctccg acctggcggt
      241 cgatccgcag gacccgcgca tcctctacgc cggcatgtgg cagttccggc gtaccccctg
      301 gtcgttcgat tccggcggcg ccggcagcgg cctctacaag tcgaccgacg gcggcgagac
      361 ctggcgcgag ctcgagaagg ggctgccggc gggggagaag gggcggatcg ccgtcgccgt
      421 ggcgccgtcg cggccgagcg tggtctatac cctggtggag gcggagaaga ccgccctcta
      481 ccgctcggac gacgtggggg agagctgggc cgaggtcaac tcctccttca ccgtcagcgg
      541 gcggccgttc tacttcgccc tggtggtcgt cgacccgacg gatttcgacc gcgtctacaa
      601 gccgggcttc accctgggcg tcagcagcga cggcggccgc tccttcggct cgccgttctc
      661 cagcttcgag aacagcgtcc actccgacca ccacgccttg tggatcgatc cccggaaccc
      721 ccagcacctg ctgctgggca ccgacggcgg cctctatatc tcctacgacc gcgggcgccg
      781 ctggcggatg gtgcgggggc tgccgatctc gcagttctac cgggtgagct acgacatggc
      841 cgagccgtac aacgtctacg gcggcctgca ggacaacggg tcgtggcgcg ggccgtcgcg
      901 gagcgtcgcc ggcatccaga accgggactg ggagaacatc ggcatgggcg acggcttcta
      961 tgccttcgcc gacccggcgg accccgagta cgcgtatgtc gagtaccagg ggggcatgat
     1021 ctcgcggctg cggctgtcga ccggcgagcg caaggagatc cgtgccctgc ccagggaggg
     1081 cgaggagggc ctgcggttca actggaacac gccgatccac ctgagcccca acgagaacgg
     1141 gacgatctac ctgggctcgc agtacctgct gcgctcgcgt gaccgcgggg actcctggga
     1201 gcggatctcc ccggacctca cgaccaacga ccccaggaag cagcagcagg cgacctccgg
     1261 cggcctgacg atcgacaact ccacggcgga gaaccacacg acgatctatg ccatcgccga
     1321 gtcgccgaag aacggcaagg tgatctgggc cgggaccgac gacggcaacc tgcaggtgac
     1381 ccgcgacggc ggcgccacct gggccaacgt ggcggcgaac gtgccggggg tgccgcgcgg
     1441 cacctgggtg tcgggggtgg aggcgagccc gcacgacgag gccaccgcct tcgccacctt
     1501 cgacgtccac gccaccggcg acatgaagac ctacgtctac cggaccaccg acttcggccg
     1561 cacctggacg gcgctgatgc cggctgccat cccggaggcc gctggcacgg gcgagacgct
     1621 cgacggctac gcgcacgtga tccgccagga cgtcgtcaac ccggacctgc tcttcctcgg
     1681 caccgagacc gggctcttcg tctccatcga cggcggcgcc cgctgggccc ggttcaccgg
     1741 caacctgccg cgggtggcgg tgcgggacct ggcgatccat ccgcgggact cggacctgat
     1801 catcgccacc cacggccgcg gcatctacat cctggacgac atcacgccgc tgcgggcgct
     1861 cacccgcgag gtgctggacg ccgaggtggc gctgctgccg tcgcggccgt cggccctggc
     1921 gatcccggcc tccatccagg acttcgcggg ggacgacgag ttcgtcggcg ccaacccgcc
     1981 ggaagccgcg gccatcacct attacctgaa gaagcggcac ctgttcggcg acctcaagct
     2041 cgaggtctac gacgcccagg gggcgaagat cgcgacgctg cccggcggca agcggcgggg
     2101 gatcaaccgc gtcgcctggc cgatgcggct gccggcgccc aagctgccgc cggccaacag
     2161 cctggtggcc aaccagttca ccttcgtcgg gccgcgggtg ccggccggcc cctacatggt
     2221 gaagctggtc aagggggacc agacgctcac cggcgaggtg cggctggtcc cggatccccg
     2281 ctcgacccac cccgaggccg accggacgct ccagcagcgg acggcgatgg agctctaccg
     2341 gcggctcgag gatctcacct atgtggtaga cgcggccgtg gcggcccggg acggcgcccg
     2401 gcaacgggcg gcggcgctgc ccgcggggga ccggctgcgc cggcggctgg aggcactggc
     2461 cggcgagcta gagaccttcc gcaacgggct ggtggcctcg ggagaggggg ggatgctctc
     2521 cggcgaggag cagctccggg agaagctcgg cgacctctac ggcgcggtca acggttacga
     2581 ggggaggccg acgcagtcgc agatcgacta cgcgcgggtg ctggatgggg agttggagcg
     2641 ggcggccgcg cggttcgagg cgatccgcgc cagcgccctg tcggcggacg gcgagatcgg
     2701 ccgggagctg gcgcgccgca agctcgagcc catccgggtg ccgagccgcg aggagtggca
     2761 gaaggagcgg gagaagggct gagcctgaag acgaaaaggg aggagccttt gggctcctcc
     2821 cttccgtcgc gaagattctc ggttcgagtc gcggtggccg atatcacgcc ggatatcagg
     2881 ccagaccctg gcgccgctct tccatcagct tgcgggcgat cttcttcttg gcctggatcg
     2941 tcttgcgctt ccgcttgacc gaaggcttct cgaaatgctg ccgcttacgc agctccgtca
     3001 ggatgccgga cttctcgacc ttccgcttga agcggcgcag ggcgttctcg aagctctcct
     3061 cttcgcgcac gtgcacaacg ggcatcagca actcacctct cttctcatct gcggattacg
     3121 acccctaccc cttatgcctc ccaggggtga atggagtcgc acagttcctt gtaattttgc
     3181 ttattccgtg tcggaagtca agccgggcga ggcggggggc cggcgcgccg gcggtgccgg
     3241 acgagccggc gaccggccag ccggggcggc ggcgggagcg ggggggacgg cgtcgaagtc
     3301 gcgctcgtag acccgggcgg ggccggagcc ggagcgctcg aaccagacgg ccgccgcggc
     3361 gccttcgggg cctttggggt agaaccaggc ggagaccttg cgctccgtgt actcgcggac
     3421 gttgtgcagg ttgggctgcc cgaggagggt gcgcacctcg tcctgagtca tcccctcctt
     3481 gacctgggag aagcgctccc ggctcatgta gcgcatcccc cgggccttct ccagctcctg
     3541 gcgcaggcgg gggttgccgg ggtcgacgga gagcgccgcc tggtagatct cgatggcgcg
     3601 ccggtagtcg ccgccgcgct ggatgaagtc ctgggcgacc aggatctcct cgtcgctctt
     3661 catgcgcagg gcctcctgct ggcgcggcgt catcggacgc ccctcgaccg gcgggtcggc
     3721 gttgatgaag tcgacgaggc ggcggcggag ctcggtggag agcgcgtcga cctcctggcg
     3781 ccgccgagcg acgtcgtcct gcggggcctg gggacccgcc tgggcgttgg gctgggtggc
     3841 cggctggggc gcggcgctgc gggcggccgc ctcggcctgc gcgagctggg cgcgcttctc
     3901 ttccaggctg cggcggctgt cctggagcca gcgccactcg gcgtcggcgg cgagggcggt
     3961 gggggcgggc ttcgcctctt cgtccggccc gcagccggtg gctccgaagg gggagagggt
     4021 gagggccagc agggccgcga gccccgtctt tcctagccgt ctcccgagcc gctgcggact
     4081 gaaacctggg cgcatccgta cctccgatcg ctggccgcgg cgacggtagc acgggggccg
     4141 gcggtccgcc aagcgcccca cgtgcgccca aagcctttgt ccgcaaagga ttccgctgaa
     4201 atagagcctt gacatggcgc gtggccgcgg gaataatcga agttccttca tatgcgagca
     4261 cccgccttga ggattggctc gaggaactga ctcgaggaac cgaagaggac caggacagac
     4321 ggcgaggcag gccggcggag agcagaggat gtccctttcc gagagagtgg aacagctgaa
     4381 ccgggagatc ggagacaccg tccagcgggc ggtctccgac ctgcggcggg agatctccgc
     4441 gcggctgcgc gagagccatg acgagatcct gcggcggctc gaggacgtga gcccgccgct
     4501 gccgggggcc tacctggcgc acgatcactt cgcgccgtct ctggccgagg ccgaggcgac
     4561 cgccgaagcc cgtggcgagg cccgcgggga agcgcgcgcc ggcacggcgg cccgccgcgg
     4621 cgccttcggc gagctgcgcg acagcctggc cgccatcgac cgcgcccgct cccaggcgga
     4681 gatcctcgcg gcgctgctgg agcagacggg ccgcttcgct tcccgcgcgg cgctgctgct
     4741 gttgcgcggc agcgaggtcc gcggctgggg cggccacggc ttcggcgacg ccgacgccgc
     4801 gttgcgcggc ctggccctgg acggcacgcc gggcggtccc tgggagcgct tggccggcgg
     4861 cgccggcacc cagcgcctct cggccgccga ctgcgccgcg ctgtgcagcc gcatcgacag
     4921 cccgctgccc accggcggcg tgctgatccc catgctgctg cgcgaccgcg tcgccgccat
     4981 cctctatgcc gacagcctgg agggggccgg cctgcagccc gacgcgctcc aggtcctgac
     5041 ctatacggcg gcgctcgcca tcgagaccct gccgttccgc gagcgcgcct ccaccgcgac
     5101 gctcgagccc gcgcctccgg cggcggacga ggacgagggg acggccgcgg cggcgccccc
     5161 gccggtcagc gagcccgcgg cggccatgcc cgcctgggct ccggagcccg agcccgctcc
     5221 ggagccggag cccgcgtctt ccttcgtcgg cgagccggaa gcgccggcgc actacgccga
     5281 gtacgcggag atcgaggccg agcccgagat cgaagccgtg accggcaccg agcccgaggc
     5341 gtaccaggag gccgcggccc cctgggcacc cgccgagccg tcgtacgagg ccccggccga
     5401 ggacgccctc gtctatgccc cggcccccgc ctacgagccc gagcccgaag gcgacgtgac
     5461 ggcggctgag gagcccgccg cggcctgggg cggcggctac gcggccactg cggtcgcgga
     5521 gcccgagccc gaggtgatgg tggaggcgga gcccgaacag tcctacacgg ccgagctgcc
     5581 gcgcaccgac ttcgcggccc agccggcgtt cgcggccgcg gctccggtgg ctgagccggt
     5641 ggcggccccc gggccggcgg cctcggagac cgtcctgctg ccgcgggccg gcctgcgcga
     5701 ttcgggcgtc ctcgagcccg cccgcgagcc ggcgccggtg acgccgatcc gtcccgtccc
     5761 cccgccgccg ccgcgcgaga ccgacgaggc cgccgccggc ggcacctccg aggtgcggcc
     5821 tcccaccgac gtccagggcc cgggctgggc ctttgccacc acccgcgtgc cggtctctcc
     5881 gagcgacgag actctgcacg aggaggcgcg ccgcctggcc cgtcttctgg tgagcgagat
     5941 caagctctac aatgaagagc aggtcgaaga agggcgtcgc aaccgggacc tgtacgagcg
     6001 gctcaaggag gacatcgacc gctcgcgcca gatgtacgag gagcgtgtcg actcgcgcat
     6061 cctgcggtcc acggactact tctaccagga gctggtccgg atcctggccg ccggggattc
     6121 ccggacgttg ggcatttgag atgccgctgg ggatctaatg ccgccgggaa tgggaacctg
     6181 aagacgccac tgttgtaacc ctccgggcgg cgcagttgcc gcccggacaa cgaggtcgaa
     6241 gcgacgtgag aagctcaaga atccgccggt cccgccgtat ccccttctcc accgtcagcc
     6301 tcgtgctgct cctgccgttg ctgctcggct cgtcgccgcg cacgccgctc aagccgagcg
     6361 tcttcccgcc ggtgccggtg ccgagcttct cggaccgcgt caccctccag tacgagcagg
     6421 ccgcctccgc gctgcggcgc aacgactgcg ccggcgcggc caaggccctg acgccggtga
     6481 tggcctccaa ggggtccgag gccggcttcg cccggctcct ctcgggcctc tatgcccact
     6541 cctgcgagca ggtgccgctg gccgaggagc ggcttttcgg cgccgccgat ccggacggcg
     6601 tgctggagga ctggcggctc tacctgttga gcgacgcggc catggcgcgc ggccacgtgc
     6661 tgctggcgca ggcctccctc gccaagctgc tcggcgacta cccggcctcc cccctgcgcc
     6721 cgcgggcgct gctcaaggcc gccaccctgg cctgggagcg cggcgattcg cagcgggccc
     6781 aggagctgat cgaacagaag cggcgcgagg acctccaggg ggaggacgcg gtcgcgctcg
     6841 agaagctcgc ctgggagatc gccaaccgcc tgggtgaccc ccggctgcag cgcgacgccg
     6901 cccgccagct cctgatccag gcgccgacgg tcgcctcgtc cgtgcaggcg gccgaggtct
     6961 tccggcgtcc cgacggccag ctcgagtggt cctccttcct gaccgcgcag cagatcgagc
     7021 ggcgggccga gaccctgctg ggcatgaagc tggcgtccaa cgccctcgga gtgctggacg
     7081 ccgtccgccc ggccgaacgc gacgccgcgt ggtacctgct caaggcgcgg gcgctcaccg
     7141 cgatgaaccg cgggcaggag gcgctggcgc tgctctcctc ggtcaccggc gccgacccgg
     7201 cgcagacggc ggccctctac tggcaacggg cccaggcggc ggagaacgtg ggcacggccc
     7261 agcgcggccg ggagaacctg ccggccgccg agcgccagcg ctaccgctgg atggcgcagc
     7321 agaacctcga gaaggtggtg gaggccggcg gcgacaagga cctgtcggtc aaggccctgc
     7381 gctccctcta cgccgacgcc cagcaggacg gcagcttcga gcgcggcctc gagctcctgc
     7441 gccggctgcg cgcggtcgat cccgccgaca ccaccggcgc ggccaacctc tggaaccttg
     7501 gctggcgcga gtacaaccag cgcaactaca ccggcgcggt cggctactgg accgagctgt
     7561 tcgcccttta cccgcgcgag cacaacggcc gccgcggccg ctactggacc ggccgcgcct
     7621 tcgaggcgct gggcgagacc gagcgggcga accagatcta tgccgaggtg gcgggcgccg
     7681 acaccaccga cttttaccgc aagaacgccc tcgcgcggct gcgccgccag ccggttacgg
     7741 cggggggggc ggccagggag cgcgacgtcg agccctggct cgccgacccg gtgctgtcgc
     7801 gggcgcggct gctgaccgac gtcgggctcg acgagctggc gatgtcggag gccgcgctgg
     7861 tcgccggcaa ggcggagccg cgggcgatgc gggccctgga ggccctggtg ctgggccgca
     7921 agggggaccg ccgcaagagc gtccaggtga tccgcgacgc ctacccggcc ctgggcggcg
     7981 ccttccaggc gggcctgccg gtgcaggccc ggcagctcta ctatccgctc gactaccagg
     8041 agcaggtccg cgcctgggcg gggaccaacc acctccccgc ctacctggtg ttcggcatca
     8101 tccgccagga gagcgccttc gacaccaacg cccagagctg ggcgggggcg cgcggcctga
     8161 tgcagctcat gccggcgacg gcgcgtgagc tggccggcaa gttcggtctg ccctactcgc
     8221 acgagaagct gtccgaaccg gcctacaacc tgcgtctggg gaccagctac ttccggcagg
     8281 tgctctcgat gttcgacggc aacgtcgagc tcgccctcgc cgggtacaac ggcggcccgt
     8341 accgcatgaa gcggttgtgg gccgagtcca acggcgagct cgaccgcttc ctcgagagcc
     8401 tgacgatcga agagtccaag acctacgtca agcggatcct ggtgctctcc gacagctacc
     8461 ggcagctcta cccgcacgtc ggctgaggcg gcggcccctt tgcccatcgc caccgtctgc
     8521 cggaggctcg cgaccaccgt cctgccgctc ctcctcctcg cctgtggagg aggagccccc
     8581 tcgccaggga ccccagccgc cggcgactcc acgggtgccg gcggccccat cgtcctcatc
     8641 accttcagct ccctgcgcgc cgacgtggtg ggcggcctcg gcggtctgcc cgggctgacg
     8701 cccaacctcg acgccctgat ccgccaggcc gattggtccg gccgcgccat cgctacgacc
     8761 agccggagcg agccggccct cgcctccctc tggaccggcc tgcgcccctg gcagcaccag
     8821 gtgttgcacg gcggcgccac cctctcgccc gacctgctca ccctggcgga ggccctcgcc
     8881 gcggccggct actcgaccgc ggggttcccc ggcggcccct ggtactcgca ggccgagggc
     8941 ttcgcccagg ggttcgagac cttcgacccg gcggaccgcg ggcggggagc gggagcgcgc
     9001 ctccgggggt tcggagccgg agaggcgcgc ggccggcagt tcgtctgggt ccacatcccg
     9061 gagccgcagg cgccgtacgt gctccggcgc cggctgctgc cgcgcgtgga gcgcctgggg
     9121 gggctgccac gggagctgcc gatgcgcatc cggccgctcc agctcgagcc ctacttcgac
     9181 cccggcgtcc cactgcccga gggcaagcgc cggcgcttct gggccatgta cctcctcaac
     9241 gtcgcctggg ccgacgagaa gctgggccgc ctgatcgagg cgctgcgggg gagcggccag
     9301 tgggaccgga cgctgctcgc ggtggccgcg gtccacggcg aggagttcgg cgagcggggg
     9361 cagatcggcc acggcggcaa cctggggcgc cgtctcctgg aggtgccgct ggtcgtcaag
     9421 ctgcccaagg aggacggcgg gcgtcgcatc gcggtgccca agggggagcg ggtggcggcg
     9481 gcccgcctgt gggcgacgct ggtcgaggcc gccggcggcc cggctggtca tccgacggtg
     9541 ccggccgccg cgccgagcct gttccggccc gccccctggc ccgccctgtc ggagctctac
     9601 ctgaccaacg gcgccaacgt cttctctctg gtggaggggg actggcagct cctctgggag
     9661 tcgcgcttcg cgccggccga gcccggctac tatcgtgccc ggtacgcggc gctgaccggg
     9721 aggcagccgg acgagctctt cgggcgcctc cggtccg
//
LOCUS       DASPJI010000023        10965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_516356, whole genome shotgun sequence.
ACCESSION   DASPJI010000023 DASPJI010000000
VERSION     DASPJI010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10965)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10965
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516356"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VEL74_00595"
     CDS             complement(<1..524)
                     /locus_tag="VEL74_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYG61051.1"
                     /translation="MALLSDDGLLARLVAFDSVSRNSNLPIADFLCDYLDGAGVRVER
                     QLSPAGDKANVIARVGPAREDGAGLVLSGHMDVVPADEAGWESDPFRLTDRGDRWVAR
                     GSADMKGFLAVATNLAAGVVAEADRLGAPLALVFTYDEEVGTLGARHLLESWSGGVSS
                     GLPRSAIIGEPTSL"
     gene            complement(562..1908)
                     /locus_tag="VEL74_00600"
     CDS             complement(562..1908)
                     /locus_tag="VEL74_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61052.1"
                     /translation="MSEWPDHPRRVSFQELVGGKASREEARTAVRHLLAGCPVCIRLA
                     EEARSGVSRTPARTYDEVFDRIERRLTDEVRKREGEKATARQLYAELLGHEAVEALLQ
                     VQSTRRYASLALCELLLKKSQSLRKDDTDRSQTAAELAQAVAEQLDFGLYGSPVVQDL
                     QASTWAYVAQAGQTQADLRAAESSYRMAERLLEPMAGGPPTPPAAELLTLQASLASYT
                     GRFEDAARLLNRAASFYRQTGDLHRFGRTLIKKGAVLGNAGELESATRLLRRGIDLID
                     GAHEPRLMVCPVHNLIWFLHESGREDQAAACLDGARRLYQRAGDRRDLGRLRWLEGKL
                     ARRLEDAERPLREAREGLAREGLSYEAALASMDLAMRYAAEQRGNEMRRYADGMLPLF
                     RSGDMYHETVQALLSFQKQDAELGTEGFLDEMGAYIRRIIAEKNPPGLLPAAAAPS"
     gene            complement(1972..5304)
                     /locus_tag="VEL74_00605"
     CDS             complement(1972..5304)
                     /locus_tag="VEL74_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61053.1"
                     /translation="MFPVLLHPDLYRRLRQSAPGVRARVRKALLRLREGHWGGGTRVK
                     RLRGMRRPVYEARSDSGDRLLFTVVRSSLPDNPDQLVCHLQVWDLVEHDDVDRAARRN
                     RSPEAEFLELDAVEEFEIHEPPPHPEASFDELPTEPGGEPDAAEPLLQFLLPPDSFAP
                     HAEEGIEGGIRWYRLDSALLAGEDEFQRLLDEGGEEIELKLTREQYEVLAAPGPVLLA
                     GSAGSGKTSIAVHRLVQASLDPEAPRCLYLSYSAPLVEHAKRLYRDVVLARGANPKQN
                     PPDFFTFGDLYLSLIPRDFKEHQARPMGEPLFREWFRKAGRSLDPALVWEELRSILKG
                     ACLSATQPMLDEETYFDLGRKRAPLFVDERPEIYRIAQRYQAWLAEEGRSDRIDLCRR
                     ALTEQRRRAKTYPVVVCDEVQDLTELEVAFVLSLSSRPDLAGVLLAGDAQQIVNPSGF
                     RWAEVRRLAGKQARAKTAPPVLRLRRNLRSVRPVVELANTLLLLRRETFGRSEEDEPE
                     EAAIAGPVPFEVAAGEQEVLDALEGFGPRCAVLTLDEEESRRLRRRLGTTRVFHVREA
                     KGLEFDSVVLWKLLAPDRDLVERFVRLPRGESRLEREPRFARLLRHLYVAVTRARRHL
                     AIYEGGTPHPFWSQERFRASLERGAVESLPRLFRATASPEVWEEEGDYFLERGHLRQA
                     AECYRRAGAAEKEAEALARVAEAAEDWATALAHWTKLGLAERQAPILERLGRYAEALV
                     LYRNAGLEREARLCELRLLERDKAWKEAAAGWEELGFFQEASRCWAQAGEERRALAAG
                     ARAAEAAGDWARAAAAWLELGDHAAAARCFAAAGDPVSAALALAHLHEKAGEWRRAAA
                     AYRRAGRATAALRCRAQFHEESGRLDRAGRAREHLGESERAVDLYARAGRWLDVARLE
                     GVGPLDRQRTLARVRELAESGGWDEAGELARTRLQALQPHLPEVPWFVAAAGERFAWQ
                     EHGLLGDLEIRCQALRAESQEAWSRATRLWRQVGEGDRAITARTRRIERIEDPLRRAR
                     AWVAAGDVERALSTLEQPGASPAAQLMAAAWKEERTERWSDAAALWKALGRPRDESRC
                     LQKATERKDRKARQPAASGPRD"
     gene            5811..7982
                     /locus_tag="VEL74_00610"
     CDS             5811..7982
                     /locus_tag="VEL74_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HYG61054.1"
                     /translation="MGERSAAWSRRIRAWVRSLDFALARRFGLATREDRVFYALIGLM
                     GVIAGLLGAAVHALIDILQALLWRSPGPVVEAAREVPRWLVVAAPATGGVLVGLIAWA
                     FRRQVGGGGMAALIEAVALSGGKVPPKPVLANALAAVATVGSGGSLGREGPMIRLGAM
                     ISSWLASRLGLPPHRLKILIGCGAAAGLAAAYNIPVGGALFAMEVILGNFALEIFGPI
                     VASSVISTLISRALSGNVPLYAAPGYALVSGWEILHYMALGVLCGGLSVVFILGVRWG
                     RRLFGSLPLPGPFKPVLGMTLLGLMALWVPHVLGGGHEAINLALQGELRLPESLALPH
                     QFTIAMLLGLAAAKLLATALTAGSGGAGGLFTPSLFVGALVGGAYGYWAHSLWPEIAS
                     PYGAYAAVGMAAILAGTSHAPLSAILILFEFTGNYDLILPLMVASILSSVVARKLRRY
                     SIYTETLRGKGVELPWRMEEAVLAGLKAEDLVRADPEVLRPGDSYRQVVDRFLGSRRQ
                     RLFVVDPAGELQGAISLHDIKGALEHPESLPAVVAHDLMLPVGQVVREGERLHRATEY
                     FAKSDFERLPVVDAAGRFLGVLAKRDVLAVYAQEVLGRPALLATYVSSNDSQTSRQYV
                     ELPPDFSLRMVPVPPELVGKTLAEASLPQTLGVRILDVDRAGGEAILPGAETVLLAGD
                     ILTLLGPTVTLEELMAGRLPVVPAAPAAAAAPAPGAGAGVE"
     gene            7988..8221
                     /locus_tag="VEL74_00615"
     CDS             7988..8221
                     /locus_tag="VEL74_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61055.1"
                     /translation="MAEEERPSARDRRRLADLAVPPGLCATCEHLRLVASKRSVFVRC
                     GLAETDPAFPRYPPLPVRACSGWRPATVSGERA"
     gene            complement(8280..8888)
                     /locus_tag="VEL74_00620"
     CDS             complement(8280..8888)
                     /locus_tag="VEL74_00620"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HYG61056.1"
                     /translation="MKRTVFVSLLLILCLTAGSLSAYVLLSPRRTWDGVPTLVVDNRG
                     NSSITDGDLGRTRTRNAINSAWNGAGSGTRVNAVVGSISGFALGDGRPYLNFTDPTGA
                     CGGSCLAATFTGYYVRRSNGTYRITDADIVTNRSYAWASLGESCSSEMYTEAVMVHEV
                     GHMLGLGHSSTSGATMYPSVSYCNNGPASLATDDINAINALY"
     gene            complement(8913..9470)
                     /locus_tag="VEL74_00625"
     CDS             complement(8913..9470)
                     /locus_tag="VEL74_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61057.1"
                     /translation="MRRLMFMALLAVGLGIVPAGASTFLALDQGELVQGSAAVIQGKV
                     IQINSFWDPTGSVVMTEALVEVHDKIVGNVPSVVAVQTFGGMVGDFYVEAHGFPKFKV
                     NEKVILFLEPEKDGAMRVAGYQQGHFRIRQDKAGREIAVPTLDLGAHLLTKDGRPGPR
                     QQAMPVDSLKDQIRTTAARAGLLTQ"
     gene            10005..10472
                     /locus_tag="VEL74_00630"
     CDS             10005..10472
                     /locus_tag="VEL74_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61058.1"
                     /translation="MNTELERPRWRSTLNYSVSPRLQVGIEYNPDAKEVGPLLTWFLL
                     TEEERRPALFLGTSSDRIGSPEGKQSYYLTASKGHPEWPVSAYVSLNYSEWDGGFNVP
                     FGANVELGRGFSLQPMYDGQRSHLLLNYDRERYGVSLLWVWLEEAGLSFNMRF"
     gene            10474..>10965
                     /locus_tag="VEL74_00635"
     CDS             10474..>10965
                     /locus_tag="VEL74_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HYG61059.1"
                     /translation="MRQRAATFLCLLLTLLILPSVIGYAADAPKSAVCPVCRVHEGES
                     EPEAVKATSVHKGETHGFCSAECKKKFDEYPEAYLPPVLPRPVPAFTVKTLDGKEVSF
                     QSLAAGKPVLVDFWATWCAPCVTAMPELQRLHQRHAAKGFSVVGISIDEEHDKAKSFA
                     RKKK"
BASE COUNT         1545 a         3873 c         3905 g         1642 t
ORIGIN      
        1 ctcagcgacg tcggctcgcc gatgatcgcg ctccgcggca acccgctgga taccccgccg
       61 gaccagctct cgagcaggtg ccgggcgccg agcgtcccca cctcctcatc ataagtgaag
      121 accagcgcca ggggagcgcc gagtctgtcc gcctccgcca ccaccccggc cgcgaggttg
      181 gtggcgaccg cgaggaaccc cttcatgtcg gcggagcccc gcgccaccca ccggtcgcca
      241 cggtcggtca gccggaaggg gtcgctctcc cagcccgcct cgtcggcggg gacgacgtcc
      301 atgtggccgg agagcacgag ccccgcgccg tcctccctgg cggggccgac gcgggcgatg
      361 acgttggcct tgtcgccggc aggggatagc tggcgctcga cacgcacgcc ggcgccgtcc
      421 aggtaatcgc agaggaagtc ggcgatcggc aggttgctgt tgcggctgac ggagtcgaag
      481 gcgaccagcc gggcgagcag gccgtcgtcg gagaggagcg ccatggcgcg gattatagtc
      541 gggcgccctc tccggggtgg gctacgacgg cgccgccgca gccgggagca ggccgggcgg
      601 gttcttctcg gcaatgatgc ggcggatgta ggcccccatc tcgtcgagga agccctcggt
      661 ccctagctcg gcgtcctgct tctggaagga gaggagcgcc tgcaccgtct cgtggtacat
      721 gtcgccggac cggaacaggg ggagcatgcc gtcggcgtag cggcgcatct cgttcccccg
      781 ctgctcggcg gcatagcgca tggcgaggtc catggacgcc agggcggcct cgtagctcag
      841 gccttcgcgc gccagcccct cgcgcgcttc gcgcagcggc cgctcggcgt cctcgaggcg
      901 gcgtgcgagc ttgccctcca gccagcgcag ccgaccgagg tcgcggcggt cgcccgcgcg
      961 ctggtagagg cggcgcgcgc catcgaggca ggcggcggct tggtcctcgc gcccgctctc
     1021 gtgcaggaac cagatcaggt tgtggacggg gcagaccatc aggcgcggct cgtgcgcccc
     1081 atcgatcaga tcgatccccc gccgcaggag gcgggtggcg ctctcgagct cgccggcgtt
     1141 gccgagcacg gcgcccttct tgatcagggt gcggccaaag cggtgcaggt ctccggtctg
     1201 gcgataaaag gaggccgccc ggttgagcag ccgggccgcg tcttcgaagc ggccggtata
     1261 gctggcgagc gaggcctgta gcgtcagcaa ctcggcggct ggcggggtcg gcgggccgcc
     1321 ggccatcggc tcgaggagcc gctccgccat ccggtaggag ctctccgccg cccgcaggtc
     1381 ggcctgcgtc tgtccggcct gggccacata ggcccaggtg gaggcctgca gatcctgcac
     1441 gaccggcgat ccgtacaggc cgaagtcgag ctgctccgcc acggcctggg ccagttccgc
     1501 cgccgtctgg gaccggtcgg tatcgtcctt ccgcagcgac tggctcttct tgagcagcag
     1561 ctcgcagagc gccaggctgg catagcggcg ggtgctctgc acctggagca gcgcctccac
     1621 cgcctcgtgc cccagcagct cggcatagag ctgccgcgcc gtcgccttct ccccctcgcg
     1681 cttgcgcacc tcgtcggtca agcgccgctc gatccggtcg aagacctcgt cgtaggtgcg
     1741 ggccggcgtc cgggagacgc cgctccgcgc ctcctccgcc agccggatgc agaccgggca
     1801 gccggccagc agatggcgca cggccgtccg cgcctcctcg cgcgaagcct tgccgccgac
     1861 caactcctgg aagctcacgc gccgcgggtg gtccggccat tcagacatgt cttgaggctc
     1921 ccccctctgt cactccgcta aattatctga agttctgaag aattcaaaga gtcaatcccg
     1981 gggtccggaa gcggcgggct gccgggcctt ccggtccttg cgctcggtgg ccttctgcag
     2041 gcaccggctc tcgtcccgtg gacggcccag agccttccag agggcggcgg cgtcactcca
     2101 ccgctcggtg cgttcctcct tccaggcggc ggccatgagc tgggctgcgg gcgaagcgcc
     2161 gggctgctcg agagtgctca gggcgcgctc gacgtcgccg gcggccaccc aggcccgggc
     2221 gcggcggagc gggtcctcga tccgctcgat ccgccgggtc ctggcggtga tggcccggtc
     2281 cccctcgccc acctgacgcc agagccgggt ggcgcggctc cacgcctcct ggctctcggc
     2341 gcgcagcgcc tggcagcgga tctcgagatc ccccagcagg ccgtgctcct gccaggcgaa
     2401 gcgctcgccg gcggcggcaa cgaaccaggg cacctcgggc agatgcggct ggagggcctg
     2461 cagccgggtg cgggcgagct cgccggcttc gtcccagcca ccgctctcgg ccagctcgcg
     2521 gacccgggcc agcgtgcgct gccggtccag ggggccgacc ccttcgagcc gggcgacgtc
     2581 cagccatcgc ccggcgcggg catagaggtc caccgcccgc tcgctctccc ccaggtgctc
     2641 gcgggcgcgg ccggcgcggt ccaggcgtcc ggactcctcg tggaactggg cgcggcagcg
     2701 gagggcggcg gtggcgcgac cggcgcggcg ataggcggcg gcggcgcgcc tccactcacc
     2761 ggccttctcg tggaggtggg ccagggcgag ggccgcgctc accggatcgc ccgcggcggc
     2821 gaagcagcgg gccgctgccg cgtggtcccc gagctccagc caggcagcgg cggcgcgggc
     2881 ccaatcgccc gccgcctcgg cagcgcgggc gccggcggcg agggcgcgcc gctcctcgcc
     2941 ggcctgggcc cagcagcgcg acgcctcctg gaagaagccg agctcctccc agccggccgc
     3001 ggcctccttc caggccttgt cgcgctccag gaggcgcagc tcgcagaggc gcgcctcgcg
     3061 ctccaggccg gcgttgcggt agaggacgag agcctcggca tagcggccga gccgctcgag
     3121 gatgggggcc tgccgctcgg cgaggcccag cttggtccaa tgcgcgaggg ctgttgccca
     3181 gtcctccgcc gcctcggcga cgcgggccag ggcctccgcc tccttctccg ccgcgccggc
     3241 gcggcggtag cactcggccg cctggcgcag gtgcccgcgc tccaggaagt agtccccctc
     3301 ctcttcccac acctcgggcg aggcggtggc ccggaacagg cgcggcaggc tctccaccgc
     3361 gccgcgctcg aggctggcgc ggaagcgctc ctgactccag aacgggtgcg gagtgccccc
     3421 ctcgtagatg gcgaggtgcc ggcgggcgcg ggtcaccgcc acgtagaggt ggcgcagcag
     3481 gcgggcgaag cggggctccc gctccagccg cgactcgccg cgcggcaggc gcacgaaacg
     3541 ctccaccagg tcgcggtccg gcgccagcag cttccagagg acgacggagt cgaactccag
     3601 ccccttggcc tcgcgcacgt ggaagacgcg ggtggtgccg agccggcgcc ggagccgccg
     3661 cgactcctcc tcgtcgagcg tcagcaccgc gcagcgggga ccaaagcctt ccagggcgtc
     3721 gaggacctcc tgctcgccgg cggccacctc gaacggcacc ggcccggcga tggcggcctc
     3781 ctcgggctcg tcctcctcgc tacggccgaa ggtctcccgg cgcagcagga gcagagtgtt
     3841 cgccagctcc accaccggcc ggacgctgcg caggttgcgg cgcagccgca gcaccggcgg
     3901 cgcggtcttg gcgcgcgcct gcttgccggc taggcgccgc acctcggccc aacggaagcc
     3961 gctcgggttg acgatctgct gcgcgtcgcc ggcgaggagc acaccggcca ggtcgggccg
     4021 cgacgacagg gagagcacga aggcgacctc gagctcggtc agatcctgca cctcgtcgca
     4081 gaccaccacc ggataggtct tcgcccggcg gcgctgctcg gtgagcgccc ggcggcagag
     4141 gtcgatgcga tccgagcgcc cctcctcggc cagccaggcc tggtagcgct gggcgatgcg
     4201 gtagatctcg ggccgctcgt cgacgaacaa cggcgcgcgc ttgcggccca gatcgaagta
     4261 ggtctcctcg tcgagcatcg gctgggtggc ggagagacag gcccccttga gaatgctgcg
     4321 cagctcctcc cagaccagcg ccggatccag cgagcggcca gccttgcgga accactcgcg
     4381 gaacagcggc tcacccatcg gacgtgcctg gtgctccttg aagtcgcggg ggatgagcga
     4441 caggtagagg tcgccgaagg taaagaagtc cggcgggttc tgcttcgggt tggcgccgcg
     4501 ggcgaggacc acgtcgcggt agaggcgctt ggcgtgctcc accagcggcg cgctgtacga
     4561 gaggtagagg cagcgcggcg cctccgggtc gagggacgcc tgcaccagcc ggtgcacggc
     4621 gatgctggtc ttgccgctgc cggcgctgcc ggcgaggagg accgggccgg gggcggccag
     4681 cacctcgtac tgctcccggg tcagcttgag ctcgatctcc tcgcccccct cgtcgagcag
     4741 gcgctggaac tcgtcctcgc cggcgaggag cgccgagtcg agccggtacc agcggatgcc
     4801 cccctcgatc ccctcctcgg catgcggggc gaagctgtcc ggtggcagga ggaactggag
     4861 aagcggctcg gcggcgtccg gctctccgcc gggctctgtg ggcagctcgt cgaagctcgc
     4921 ctccggatgg ggcggcggct cgtggatctc gaactcctcg accgcgtcga gctccaggaa
     4981 ctcggcctcg ggcgagcggt tgcggcgcgc cgcgcggtcg acgtcgtcgt gctccaccag
     5041 gtcccagacc tgcagatggc agacgagctg atccgggttg tccggcaggg acgagcgcac
     5101 caccgtgaac agcagccggt cgccggagtc ggagcgcgcc tcgtagaccg gccgccgcat
     5161 gccgcgcagc cgcttgaccc gcgtgccgcc accccagtgc ccttcccgca gacgcagcag
     5221 cgctttccgg acccgcgcgc gtacaccggg ggcgctctgg cgtagccggc gatacaagtc
     5281 gggatgcagg agcaccggaa acatcgtgcg cagctccatt caacggtatg cctgtcaggg
     5341 aaaggcatga ccaggaatga gcaggattgt aagcccgggg agggttttcg tccaagcgaa
     5401 acgcctgtga gcctccggaa aggcggaacg agcgtccgga agaaggaggc tggaaaggcg
     5461 aaggggaccg gaaagctccg gtcccctcga aaacttgaca cgcacgaccg gccgggccgt
     5521 ggtgacggaa ggggggtacc gcggggcgtg acttccgccg tgaggccccc atgaaccggc
     5581 agcggacgcg aaccggcggg gcctcgggag gcttcggtcg cctcgggcga ccacgacgag
     5641 gtagttatac accaatgagc atctgtatgg aatagcaaaa tccaactgcg tctgtggaag
     5701 ggagccacca ccccggggcc aaccccctcc tcctccatct atatagatat agatgggggc
     5761 gatccacctc tcccgggcgt cctgccccca cacctgatac attccggccc atgggagagc
     5821 gctccgcggc gtggtctcgc cgcatccggg cgtgggtccg ttccctcgac ttcgccctcg
     5881 cccgccgttt cggcctggcg acgcgcgagg accgcgtctt ctacgcgttg atcgggctca
     5941 tgggggtgat cgccggcctc ttgggcgcgg cggtccacgc attgatcgac atcctccagg
     6001 cgctgctctg gcgctcgccg gggccggtgg tggaggcggc gcgagaggtg ccgcggtggc
     6061 tggtcgtggc cgcgccagcg accggcggcg tcctggtggg gctcatcgcc tgggccttcc
     6121 gccggcaggt ggggggcggc ggcatggcgg cgctcatcga ggcggtggcg ctgtcggggg
     6181 gcaaggtgcc gccgaagccg gtgctcgcca acgcgctggc ggccgtcgcc acggtcggca
     6241 gcggcggctc gctggggcgc gagggcccga tgatccggct gggcgccatg atctcctcct
     6301 ggctggcgtc gcggctgggg ctgccgccgc accggctcaa gatcctcatc ggctgcggcg
     6361 ccgcggcggg cttggcggcg gcctacaaca tcccggtggg gggcgcgctg ttcgccatgg
     6421 aggtcatcct cggcaacttc gcgctggaga tcttcggccc catcgtcgcc tcctcggtca
     6481 tctcgaccct gatctcacgg gcgctctccg gcaacgtgcc gctctatgcc gctcccggtt
     6541 atgccctggt cagcggctgg gagatcctcc attacatggc gctgggcgtg ctgtgcggag
     6601 gcctttcggt tgtgttcatc ctgggggtgc ggtgggggcg ccgcctcttc ggctcgcttc
     6661 ccctgcccgg gcccttcaag ccggtcctcg ggatgacgct cctcgggttg atggcgctct
     6721 gggtgccgca cgtgctgggt ggcgggcacg aggccatcaa cttggcgctc cagggggagt
     6781 tgcggttgcc ggagagtctg gctctgccgc accagttcac catcgcgatg ctcctcggcc
     6841 tcgccgccgc caagctcctc gccaccgccc tgaccgcggg cagcggcggc gcgggggggc
     6901 tgttcacccc ctccctcttc gtcggcgcgc tggtcggcgg cgcctacggc tactgggccc
     6961 actccctctg gccggagatc gcctcgccct atggcgccta cgcggcggtg ggcatggcgg
     7021 cgatcctcgc gggaacgagc cacgcgccgc tctccgccat cctcatcctc ttcgagttca
     7081 ccggcaacta cgacctgatt cttccgctga tggtggcgtc gattctgtcg agcgtggtgg
     7141 cgcgcaagct gcggcgctac tccatctata ccgagacgct ccgcggcaag ggggtcgagc
     7201 tcccctggcg catggaggag gcggtgctcg ccgggctcaa ggcggaggac ctggtgcgcg
     7261 cagacccgga ggtgctgcgg ccgggcgaca gctaccgcca ggtggtggac cgcttcctcg
     7321 gctcccggcg ccagcgcctc ttcgtcgtcg atcctgccgg cgagctccaa ggggcgatct
     7381 cgctgcacga catcaagggg gcgctggagc acccggagag cctgcccgcg gtcgtggccc
     7441 acgacctcat gctgccggtg gggcaggtgg tccgggaggg cgagcggctg caccgcgcca
     7501 ccgagtactt cgccaaaagc gacttcgagc ggctgccggt ggtggacgcc gccgggcgct
     7561 tccttggcgt cctcgccaag cgggacgtgc tcgcggtcta cgcgcaggag gtgctgggcc
     7621 ggccggcgct gctcgccacc tatgtctcga gcaacgattc gcagaccagc cgccagtacg
     7681 tggagctccc ccccgacttc tccttgcgca tggtgccggt gccgccggag ctggtgggta
     7741 agaccctggc cgaggcgagc ctcccccaga ccctgggcgt ccgcatcctc gacgtcgacc
     7801 gtgctggcgg cgaggcgatc ctccccggcg ccgagaccgt gctcctggcc ggggacatcc
     7861 tgactctgct cggccccacc gtcacgctgg aggaactgat ggccggccgg ctccccgtcg
     7921 taccggccgc ccctgccgcc gccgcggcgc cagcgccagg ggccggcgcc ggagtggagt
     7981 agagaggatg gcggaggaag agaggccctc ggcgagggac cgccggcggt tggccgatct
     8041 cgccgtgccg ccggggctct gcgccacctg cgagcacctg cgcctggtcg cctcgaagag
     8101 gagcgtcttc gtccgctgcg gcctggctga gaccgatccc gcgttcccgc gctacccacc
     8161 tttgccggtg cgggcctgct ccggctggcg gccggcgact gtttccgggg agagggcctg
     8221 aaaaggccgg gggcccctga gggcccccgg gtactaggga gattgccgga cgatccggct
     8281 tagtacaggg cgttgatcgc gttgatgtcg tcggtcgcga gcgaggccgg accgttgttg
     8341 cagtagctga ccgacgggta catcgtggca ccgctggtgc tgctgtggcc gaggcccagc
     8401 atgtggccga cctcgtgcac catgacggct tcggtgtaca tctcgctgga gcaggactca
     8461 cccagcgagg cccaggcata ggaccggttg gtcacgatgt cggcgtcggt gatgcgatag
     8521 gtgccgttgc tccgccggac gtagtagccg gtgaaggtgg cggccaggca ggagccgccg
     8581 caggcgcccg tggggtccgt gaagttgagg tacggccggc cgtcgcccag ggcgaagccg
     8641 gagatcgagc ccaccaccgc attgacgcgg gtgccggagc cggcgccgtt ccaggcggag
     8701 ttgatggcgt tgcgggtgcg ggtgcggccg aggtcgccgt cggtgatgct ggagttgccg
     8761 cggttgtcga cgacgagagt gggcacgccg tcccaggtcc ggcgcgggct gagcaggacg
     8821 tacgcggaaa ggctacccgc agtcaggcag aggatgagga gcaggctgac aaaaaccgtt
     8881 ctcttcatga tccaggttcc attccttaga agttactggg tcaggagccc ggcgcgcgcc
     8941 gccgtggtcc ggatctggtc cttgagggag tcgacgggca tcgcctgctg gcgcgggccc
     9001 ggacggccgt ccttggtcag gagatgagcg cccaggtcga gcgtcgggac cgcgatctcg
     9061 cggccggcct tgtcctgacg gatccggaaa tgaccctgct gataccccgc gacccgcatc
     9121 gcaccatcct tctcgggctc gaggaagaga atcaccttct cattgacttt gaacttgggg
     9181 aagccgtggg cctcgacata gaagtcgccc accatgccgc cgaaggtctg caccgccacg
     9241 accgagggga cgttgcccac gatcttgtcg tgcacctcga ccagagcctc ggtcatcacg
     9301 accgagccgg tgggatccca gaacgaattg atctggatga ccttgccctg gatgaccgct
     9361 gccgagccct ggacgagctc gccctggtcc agcgccagga aggtcgacgc tccagccggg
     9421 acgatcccga gacctaccgc caagagagcc atgaacatca gacgcctcat gcaacctcct
     9481 tgtcctacca aaggacgcga agcaaagcca ccgactatcg gcagctttag aaacgatgtt
     9541 aagacgccaa cgcttaggca ccgtcgtgga taaacccccg gtgccagtct cagagccggt
     9601 cgccgcttac cgagccggcg gccaactaaa acttcggatc tctcggcccc tcccgccacc
     9661 ccactgggtc ctacgaaaag ccttctactt gaggtggtgc acgagaccgg gaccgcgtga
     9721 caagtttttt cagaaaaatt cacccccgcg acaccgcggc gccccagcgc gccagcggat
     9781 accccgaacg gtgtatcctc ccgggcatga ggtgccgcct gccgctcctg gctctcctgc
     9841 tcctggtccc cctggccgtc cccgcccagc cacccgagga gccggagacc cctgcccctc
     9901 cgcgccgccc ctcctccgcg ccgacgtgaa gcggctgacc cggcgagggc gcgccgctcc
     9961 gggggagcgg cctggattcc agatggacct tctcggggcg cttcgtgaac acggagctgg
    10021 agcgcccgcg ctggcgctcc accctcaact acagcgtcag cccccggctg caggtgggaa
    10081 tcgagtacaa cccggacgcc aaggaggtcg ggccgctcct cacctggttc ctgctgacgg
    10141 aagaagagcg ccgccccgcg ttgttcctgg ggacgagctc ggaccggatc ggctcgccgg
    10201 aggggaagca gtcgtactac ctgaccgcct ccaaggggca tccggagtgg ccggtctcgg
    10261 cctatgtgag cctgaattac tcggagtggg acggaggctt caacgtcccg ttcggggcga
    10321 acgtggaact gggccggggg ttctcgttgc agccgatgta cgacgggcag cggtcgcacc
    10381 tgctgctcaa ctacgaccgt gaacgctatg gggtgtccct cctctgggtc tggctggaag
    10441 aggcgggcct ctccttcaat atgaggttct gagatgagac agcgagccgc gacctttctg
    10501 tgcctgctcc tgaccctgct gatcctgccg tccgtcattg gctatgccgc cgatgcgccg
    10561 aagagcgccg tctgccccgt ctgccgggtg cacgaggggg agtccgaacc ggaggcggtc
    10621 aaggcgacca gcgtccacaa gggcgagacc cacggcttct gctcggcgga gtgcaagaag
    10681 aagttcgacg agtacccgga ggcctacctg ccgccggtgc tgccgcgccc cgtgccggcg
    10741 ttcacggtca agaccctcga cggcaaggag gtctccttcc aatcgctggc cgcgggcaag
    10801 ccggtcctgg tcgacttctg ggccacctgg tgcgcgccct gcgtcaccgc gatgccggag
    10861 ctgcagcggc tgcaccagcg gcacgccgcc aagggcttct cggtcgtcgg catctcgatc
    10921 gacgaggagc acgacaaggc gaagtcgttc gcccgcaaga agaag
//
LOCUS       DASPJI010000024         4410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_231330, whole genome shotgun sequence.
ACCESSION   DASPJI010000024 DASPJI010000000
VERSION     DASPJI010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231330"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3159)
                     /locus_tag="VEL74_00640"
     CDS             complement(<1..3159)
                     /locus_tag="VEL74_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG61060.1"
                     /translation="MSRPPVFVLSPPRSGSTLLRVMLAGHPRLFAPPELELLSFETMG
                     DRKAAFTGRDAFWLEGLVRAVMEVRGLDAGEAGEIVAGFERDDRSTLEMYRALQEWLG
                     DRLLVDKTPSYALDSEVLERAERELGEPFYIHLARHPCGMIRSFIEAKLDQIFFRQEH
                     DFSRRELAELIWVVSHQNITDFLAGVPAERQMLIRFEDLVRDPEGVLGRLCARLGIGF
                     EPAMARPYENRERRMTDGIHAESRMLGDVKFHQHSGVDPAVAESWRGEEREDLLGDLT
                     RQVAELLGYRRGYRRESAATALAAIEPLASTAGGPQPLSFAQERLWFLDQLEPGSPAY
                     NIPAALRLSGRLGLPALAASLEEIVRRHASLRTRFAAVEGRPVQIAEPAAGVPFPLVD
                     LGGLPTDVRDGEALRLAREEALRPFDLARGPVLRAALLRMSGEEHAALFTLHHIVSDG
                     WSVGVLIREVGALYEAFSAGRSSPLPELPIQYADFALWQRSHLAGEALETQIAYWRER
                     LMGAPPVLELPTDRSRATAPGGRGGTRAALLAVRLEIPPGKTPFMVLLAAWMVLLHRI
                     SGQSGLVVGTPVAGRHREEVEELIGFFVNTLPLRVDLSGDPTFPELMERVRSVVLGAF
                     AHQDLPFEKLVEELAPERRRARTPLFQVLFALQNARGGGLSLPGLTLVPFEVPGETAK
                     FELTLSLEEVEGGLAANLEYDRDLWDAATIDRMLGHLGSLLAAAPGARLSDLFLLRAT
                     ERQQLLLEWNDTTTAYPRDRSIAELFAAQAAACPDSVAVAWDERSLTYGELARRARLL
                     AGCLRRLGVAPEVPVGLFAERSAEMVVGLVGILEAGGAYVPLDPSYPGERLALLLDDA
                     RVPVLLAEPRLLGRLPVHGAAVLPLEEGADAYREGAEALPRGSAGPDHLAYVIYTSGS
                     TGRPKGVAVPQRAVARLVLDTDYVRLGPGDRVLQASNSSFDAATFEVWGALLCGATLV
                     GVPRELLLSPARLAGLLREWEGGTLFLTTALFQQLSREEPGIFAPLRDLLFGGEAVDA
                     AAVRALVTNGRPPARLLHVYGPT"
     gene            complement(3156..>4410)
                     /locus_tag="VEL74_00645"
     CDS             complement(3156..>4410)
                     /locus_tag="VEL74_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HYG61061.1"
                     /translation="EGVTVLNQTPSAFGVLAQAEETAEETAPLRLVIFGGEAVEPAAL
                     SPWCRRHGEERPSLVNMYGITETTVHVTWRRLGGTDLREGSGSPIGAAIPDLRLYVLD
                     GHGQPVPVGAPGEIHVGGAGLARGYLGRPDLTAVRFLPDGWSGGVGERLYRTGDLARY
                     RPDGDLEFLGRIDHQIKIRGFRIEPGEIEAALLRHPRVRQAVVLAGDGPGGPGDQRRL
                     VAYVVPRPVEEGTGVLALAPLRSYLTSWLPDYMLPAALVVLESLPLTANGKVDRRSLA
                     ATRPAEEEVGGPAYEEPVTPLEHYLAGLWRQALGRERVGRHDDFFALGGNSISGAILI
                     NRLQRELGEIVHVVTIFDAPTVAGLSDYLIAQHREAVARVWGRESLGEKPVPALAAMS
                     EARVDVDKVERMRQLIRPLSSRRRA"
BASE COUNT          719 a         1620 c         1521 g          550 t
ORIGIN      
        1 ggtgggtccg tagacgtgga gcagccgagc cggaggccgg ccgttggtga cgagggcgcg
       61 aacggcggcc gcgtccaccg cctcgccgcc gaacaggagg tcgcggagcg gggcgaagat
      121 ccccggctcc tcgcgcgaga gctgctggaa gagggctgtc gtcaggaaga gggtgccccc
      181 ctcccactcg cggagaaggc cggccagccg ggccggagac agcagcaact cccgcggcac
      241 ccccaccagg gtggcgccgc acaggagggc accccagact tcgaacgtgg ccgcgtcgaa
      301 cgacgaattc gacgcctgga gcacccggtc ccccggcccg aggcggacgt agtcggtatc
      361 cagcaccaga cgcgccaccg cccgctgcgg caccgccacc cctttgggcc tgccggtcga
      421 gccggaggtg tagatgacgt aagccaagtg atccggcccg gccgagccac gcgggagcgc
      481 ctcggcccct tcacggtatg cgtccgcccc ctcctccaga gggagcaccg ccgcgccgtg
      541 cacgggcagg cggcccagaa ggcgcggctc ggcgaggagc acagggacgc gcgcgtcgtc
      601 cagcagcagg gccagccgtt cgccgggata ggaggggtcc agggggacgt acgcgccgcc
      661 ggcctccagg atgcccacta gccccaccac catctcggcg gagcgctcgg cgaagaggcc
      721 caccggaacc tcgggggcga ctcccagccg gcgcaggcat ccggcgagca ggcgggcccg
      781 gcgggcgagc tcgccataag tcagcgaccg ctcgtcccag gccaccgcga cgctgtcagg
      841 gcacgccgcg gcctgcgcgg cgaatagctc ggcgatcgac ctgtcccggg ggtaggccgt
      901 cgtcgtgtcg ttccactcca gaaggagctg ctggcgctcc gtcgcccgga gcaggaaaag
      961 atccgaaagg cgcgcccccg gggcggccgc cagcaggcta cccaggtggc ccagcatgcg
     1021 gtcgatggtg gcggcatccc acaggtcgcg gtcgtactcg agattcgcgg cgagcccacc
     1081 ctccacttcc tccagcgaca aggtgagctc gaacttcgcc gtctctcccg gaacctcgaa
     1141 gggaaccagg gtgaggcccg gcaaggagag gccgccgccg cgggcattct ggagggcgaa
     1201 gaggacctgg aacagaggcg tgcgtgcccg ccggcgctcc ggcgccagct cctccaccaa
     1261 cttctcgaac ggcaggtcct ggtgcgcgaa ggcgcccagg accacggagc ggacgcgctc
     1321 catcagctcg gggaacgtcg ggtcgcccga caggtccacc cgcaggggta gggtatttac
     1381 gaagaagccg atcagctctt cgacctcttc acggtgccgg ccggccacgg gcgttcccac
     1441 caccaggccg gactggccgc tgatgcggtg gagcagcacc atccaggccg ccaggaggac
     1501 catgaagggt gtcttcccgg gcgggatttc cagacgcacc gccaggagcg ctgcgcgtgt
     1561 gcctccccgg ccccccggtg ccgtagcgcg ggaccggtcg gtgggcaact cgagcaccgg
     1621 cggcgcgccc atcagccgct cgcgccagta ggcgatctgg gtctccagcg cctcccccgc
     1681 gagatgcgac cgctgccaga gagcgaagtc ggcgtattgg atcggcaact cggggagcgg
     1741 cgacgaacgg ccggcggaga acgcctcgta gagcgccccc acctcccgga tcagaacccc
     1801 caccgaccag ccatcgctca cgatgtggtg cagggtgaag agggcggcgt gctcctcccc
     1861 cgacatacgg agcagcgcgg cgcgcagcac cggtccgcgg gcgagatcga aaggacggag
     1921 cgcctcctcg cgcgccaggc gcagagcctc gccgtcccga acgtccgtcg gaaggccgcc
     1981 gaggtccacc agagggaacg gcactcctgc agccggctcg gcgatctgta ccggccggcc
     2041 ttccacggcc gcgaagcggg tgcggagcga ggcgtgccgg cggacgatct cctccaggct
     2101 ggcagccagc gccgggaggc ccagccggcc gctcagccgg agcgccgcgg ggatgttgta
     2161 ggccggagag ccgggctcga gctggtcgag gaaccagagc cgctcctggg cgaaggagag
     2221 gggttgcggg cctccggcag tagaggcgag aggctcgatg gccgcgagcg ccgtcgccgc
     2281 gctctcgcgc ctgtagcccc gcctgtagcc cagcaactcg gcaacctgac gagtgaggtc
     2341 ccccaacagg tcctcgcgct cctcgccgcg ccagctctcc gccaccgccg gatcgactcc
     2401 tgagtgctgg tggaacttga cgtccccgag catgcgggac tcggcatgga tgccgtctgt
     2461 catcctccgc tcgcggttct cgtagggacg ggccatggcg ggctcgaagc cgatccccag
     2521 gcgggcgcag agacggccga ggacgccttc cggatcgcgc accaggtcct cgaagcggat
     2581 gagcatctgc cgctccgccg gtaccccggc caggaagtcg gtgatgttct gatggctcac
     2641 cacccagatc agctccgcca gctcacggcg ggagaagtcg tgctcctggc ggaagaagat
     2701 ctggtcgagc ttggcctcga tgaaggagcg gatcatgccg caggggtggc gggccaggtg
     2761 gatgtagaag ggctcgccga gctcccgctc cgcccgctcc agaacctcgg aatccagcgc
     2821 gtaggagggt gtcttgtcca ccaggagcct gtctcccagc cactcctgga gagcacggta
     2881 catctccaac gtggagcggt cgtcgcgctc gaatccggcg acgatctcgc cggcctcccc
     2941 ggcgtccagc ccccggacct ccatgaccgc ccgcaccagc ccctccagcc agaaggcgtc
     3001 acggccggtg aaggcggcct tgcggtcgcc catggtctcg aacgacagga gctccagctc
     3061 gggcggcgcg aacagccgcg gatgcccggc gagcatcacc cgcagcagcg tcgaccccga
     3121 gcgcgggggc gagaggacga agacgggtgg acggctcacg cgcgcctccg agacgagagg
     3181 ggccggatca actgcctcat tctctcgacc ttatccacgt cgacccgcgc ctctgacatg
     3241 gcggccaagg cgggcacggg cttctctccc agagactccc ttccccagac ccgggctacg
     3301 gcctcccgat gctgcgcgat caggtagtcc gacagaccgg cgaccgtggg cgcgtcgaag
     3361 atcgtcacca cgtggactat ctcccccagc tcacgctgca ggcggttgat caggatcgcg
     3421 ccgctgatcg aattgccgcc gagagcgaag aagtcgtcgt gccggccgac gcgctccctg
     3481 cccagagcct gccgccagag gccggccagg tagtgctcca ggggtgtgac cggctcctcg
     3541 taagccgggc caccgacttc ctcctcggcc gggcgggtcg cggccagcga acgccggtcc
     3601 accttgccgt tggccgtgag gggtagcgac tcgagcacca ccagggcggc ggggagcatg
     3661 tagtcgggca gccacgaggt cagatagctg cgaagagggg ccaaggccag aacgccggtc
     3721 ccctcctcca ccggcctggg gacgacgtag gccacgagcc ggcgctgatc gccggggcct
     3781 cccggaccgt cccccgccaa caccacggcc tggcgcaccc gggggtgccg cagcagcgcc
     3841 gcctcgatct cccccggctc gatgcggaag ccacggatct tgatctgatg atcgatccgc
     3901 ccgaggaact caaggtcgcc atccggccgg tagcgcgcca gatcgcccgt ccggtagagc
     3961 cgctcgccaa ccccgccgct ccacccatct ggaaggaagc ggaccgcggt caggtcgggc
     4021 cggcccagat agccccgcgc gagcccggca ccgccgacat ggatctcccc cggtgctcct
     4081 accggcaccg gctgcccatg cccatccagc acatagagcc gcagatccgg gatcgccgcg
     4141 ccgatcgggc tgccgctccc ctcccgcaga tcggtcccac cgaggcgccg ccacgtcacg
     4201 tgaaccgtcg tctcggtgat cccatacatg ttcacgaggg atggccgctc ctctccatgg
     4261 cgccggcacc agggcgacag ggccgcgggc tccaccgctt cgccgccgaa gatcaccagc
     4321 cgcaacggcg ccgtctcctc ggccgtctcc tcggcctgcg ccagcactcc gaacgcggaa
     4381 ggggtctggt tgagcaccgt caccccctcg
//
LOCUS       DASPJI010000025        22225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_288332, whole genome shotgun sequence.
ACCESSION   DASPJI010000025 DASPJI010000000
VERSION     DASPJI010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 22225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22225)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22225
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288332"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4938)
                     /locus_tag="VEL74_00650"
     CDS             complement(<1..4938)
                     /locus_tag="VEL74_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61062.1"
                     /translation="MSTRLIKSSAPRSGARSNLVEVLRAHALERPGRRAYTFLADGAA
                     GADGAESWTWAELDARARAIGAALARERGQGERALLLFPPGLDFIAAFFGCLYAGVVA
                     VPCYPPRPRRDHPRLRAIARDARPRFVLTTPELAARREGIAEPTPELAAASWLVPAAL
                     EPRLGEEWDEQLMEETKPETPAFLQYTSGSTALPKGVIVTHGNILHNEEMIRAAFGQS
                     EESVVVGWLPLYHDMGLIGNVLQPLCTGSSAVLMPPMAFLQRPARWLEAISRYRATTS
                     GGPNFAYDLCVRKVTPEDRAALDLSSWSVAFNGAEPVRAATLERFAETFATCGFRREA
                     FYPCYGLAEATLFVSGGQRGVLPARGYFDPTALEQGSAVPALPEESWNARALVGCGRA
                     WDGQRIVVADPETGAAVPPGRVGEVWVAGPSVAGGYWDRPEETARTFGAVLAGSPAGG
                     DRFLRTGDLGFFWDGELFVTGRLKDLIILRGRNLYPQDVELAAEASHPGLEPGGGAAF
                     SVEVDGEERLVVVHEIARRFRPEPETVATVAAAVRRVVAEELEAQIHEVVLIRTGTLP
                     RTSSGKVQRHACRAGYLAGDLEVVGRDAAAAVDGEDLQEGALPDREAFLRAELARAAG
                     VDPAAVDPGRPPVELGLDSLAAVELSHRIETALGVSIPLTRWLEAPSLAAVLEGLNPS
                     PPVPLSHPLPPTGRGENQQDVFPLSHGQRGLWFLERWAPAASAYNIPVPLRLRGDLDA
                     AALFRAVEKMAVRHPALRTTFEEQGGEPVQRVHGRLEPETFFYKDAARWSEAELGEWL
                     AREAWRPFDLGRGPLLRVALFVRGEQEHVLLLTIHHLIADFWSLAVLARELEALYRRE
                     IEGGEARLAAVSRTYADHVARERERLASGAEALWAYWGERLGGDLPALDLPGDRPRPA
                     LPTWRGGSAVLPLGVELSDGLAALARRRGATLFAALLAGFQTLLHRLTGQEDLIVGAP
                     SAGRTSADLAGVVGYFIDTLPLRADLGGDSAFGDLLGRTAGVVSGALANPVPLTLLAE
                     RLHPERDPGRSPLFQTMLVLQRAHRPADRDWSAFALGEEGARSALADLAAESVRLAER
                     RVPFDLTLMVAEEAGSHGLVASLQYALDLFDPAMAARIAGHFRTLLAAAVADPSLPLS
                     ELPLLSVAERRQVLVEWAEGPAIEPAPDRLLHELFLDQAARTPQTVALIAGEDRWTYA
                     DLAARSEAVADRLRALGVGPEDRVGICARRSPELVAGLLGILQAGGAYVPLDPAHPRP
                     RLARILADARPAAVLVGEGLEEILPEHGAQRLALSSDVGEGLAPSREGASPSPTQRLI
                     ASDTLAYLIYTSGSTGEPKGVAIRHSSAVALVRWAWEVFPEADLHRVLASTSIGFDLS
                     VFELFVPLSRGGAVVLAENVLALPELPAADEVTLVNTVPSALSTLLRTSGLPASVRTV
                     NLAGEPLTRELAARLHELPQVERVFNLYGPSEDTTYSTFSLVERGEIPTIGRPLAGTR
                     ARVLDRKLQPVPPGVAGELCLGGAGLARGYLGRPELTAERWMPDAFAGTPGERLYRTG
                     DLARFLPDGRLEFLGRIDHQVKVRGFRVEPGEIEAALVTHPGVGEAVVVARGEGGERR
                     LVAFVIRQDERVDAPALSAFLQESLPAHLVPAAFSF"
     gene            5116..8595
                     /locus_tag="VEL74_00655"
     CDS             5116..8595
                     /locus_tag="VEL74_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYG61063.1"
                     /translation="MKRDRLSEYLTSTAMDSRTESRLLRMAISRGFLQWEDLDAVADR
                     LPDRQDGRDSTQPGARWVHALLDAGRLDPRVLADLSAELERGRDDLTPELTARPVSLG
                     TSPRQLELRTTELPLTDSSSPHLSSRTSRIDPATLPVELRFLSSWERYRVERFLGAGG
                     MGTVYRAFDPFLDRWVALKFLHWNETSQVGRFINEARSQARVDHPNVCRVYEVGEVEE
                     RPYIAMQYIEGRSLSDVRDELPAEAKVRLVRDVARAVHAAHRNGLIHRDLKPGNILVG
                     TDDSGALHPFVVDFGLARDQDEDGLTRSGMLSGTPAYLSPEQAQGLPLDRRTDVYSLG
                     VVLYETLGGRPPFLAANAAGILVRLVQEEAEPLRKLQPQVPPDLETVVMKCLEKDPAQ
                     RYDSARALAEDLDRYLEGEPVHARPASWAYRLGKKARKNKALTAVAAAATIALLVLGG
                     VALRAEWQARERAALAQGFGQRVKDLEYRIRIEAFLAPHDIRAHKQQVRREAEAIRAE
                     MARLGPLAAGPGHYALGKGYLALHEYETALDHLERAWQAGQRTPEVASALGRTLQVLY
                     QKALWDAAREQNVDARRAAREESERTFSRPALSYLREIPAEETSRQPYLAALRAFFEE
                     RYPEALAKAREAYRRDVAFYEAAQLEADSLMAQGNQEGSAGRYEEALRFYDQAGETFR
                     ALLANVRSDAALYAADCNRRILRLDARMAVGGLSEAEVLDAAAACDRALQVDPELGEA
                     FTSKARLYQRWGQHQLQSGEDPTPALSDARAMAERAIALNPQDAQAYSHMAVAMRILA
                     AWEQSHGRAPGSHLEQAVQAAAKAVELQPQRATFRNNLATIYLKLAEVQMGSGLDAGA
                     AIRSGIANADQATRLDTRFGLAYINLGSAWKTMAEYQISRGDDPTESTSRGVEAFEYA
                     IRLNPNSAPAYNNLGNAHLTLGDYQMARGAAARPSLERAIASYRKAVEVRPDYEVAIF
                     NLAWTQRLLALDQLQRGEDPAPSLAAARETLGRIATDADVLLERARCDVIAARWAMRQ
                     GRESGPLLRQASSTLEQARDLNPQQADIYMEGALAARYQAEQDLKKGGRPAAAVRDGL
                     TRIEKALEINAQEARYLAEQGVLQTLAARLETDPARRRQEESRAAATLGKALEANPLL
                     RREYGPWLEGGRI"
     gene            complement(8702..9340)
                     /locus_tag="VEL74_00660"
     CDS             complement(8702..9340)
                     /locus_tag="VEL74_00660"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG61064.1"
                     /translation="MSRFGPDPLSFFEAVYRGPAPWDIGGPQPAMAGLLAEYPPEDPG
                     LDVGCGSGDLAIHLAKSGLDTLGIDFIEEAITQARAKMAALPADVADRLAFRVADALQ
                     PSLLGQTFGAVVDSGFYHLFDPEQCERFVDELARVLRPGGRCYLHEFAVTFPVPNVPR
                     QVTEAELRESFAPERGWKVLTIRTGEFLSTVAPVPAILACMERVGPEERTRL"
     gene            complement(9383..9832)
                     /locus_tag="VEL74_00665"
     CDS             complement(9383..9832)
                     /locus_tag="VEL74_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HYG61065.1"
                     /translation="MSRENNPNAAVSTERLLSANPRQVFAAFEQPDRLAQWWGPSGFT
                     NTFEQFDFKPGGRWVFAMHGPNGASNPNESVFREIQPDTRIVLEHVVQPWFRLTVTLT
                     SRGDKTHLAWVQEFESPEVAAKMRPLSATANEQNLDRLESLLATGSV"
     gene            complement(9912..10355)
                     /locus_tag="VEL74_00670"
     CDS             complement(9912..10355)
                     /locus_tag="VEL74_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61066.1"
                     /translation="MTSLLRNVLAVLAGIVIGGIVNSALITLSPSLIPPPAGVDVANA
                     ESLSQAMHLFEPRHFVMPFLAHAVGTLAGALAAYLIAASYKARIAYAMGVVFLCGGVA
                     ASFMIPAPAWFIALDLLAAYLPMAWLAIQIGARMKQGTAARRSEA"
     gene            complement(11171..11524)
                     /locus_tag="VEL74_00675"
     CDS             complement(11171..11524)
                     /locus_tag="VEL74_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016870561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HYG61067.1"
                     /translation="MPHQTQEMERCIENCQECHGVCLQNVAHCLEKGGPHAEPSHIRL
                     LLDCAEICETSANFMIRGSELHTETCRACSEVCQRCADDCRRLGSDPEMQQCAEACQR
                     CAESCAQMAGAFAHA"
     gene            complement(11673..12005)
                     /locus_tag="VEL74_00680"
     CDS             complement(11673..12005)
                     /locus_tag="VEL74_00680"
                     /inference="COORDINATES: protein motif:HMM:NF044575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61068.1"
                     /translation="MAAHAAAREVGDPAATAAARAAGLAAAVAYTHPLPTLDQAKHIL
                     GPAVYAARAREIAADGDTGVGDEEIRWAVEHASPEVREVVGRWPARGPGRSRLEALYS
                     MLDAGLRA"
     gene            complement(12056..12235)
                     /locus_tag="VEL74_00685"
                     /pseudo
     CDS             complement(12056..12235)
                     /locus_tag="VEL74_00685"
                     /inference="COORDINATES: protein motif:HMM:NF044575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12833..13099)
                     /locus_tag="VEL74_00690"
     CDS             complement(12833..13099)
                     /locus_tag="VEL74_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61069.1"
                     /translation="MEDTKPKSFVFVLMPFDESFDDVYEAGIKPACKDAGAYCERVDE
                     QIFVESILARVYNQIAKADVIIAEMTGRNPNVFYEVGYAHALNK"
     gene            complement(13124..14023)
                     /locus_tag="VEL74_00695"
     CDS             complement(13124..14023)
                     /locus_tag="VEL74_00695"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HYG61070.1"
                     /translation="MKVFLSHSSRDKKFVVRLYEALSLEGVETFLDDRDIRVGDDIRL
                     SIERGISEATHLAYIISSHSLKSRWVQEELSIAKFRAMGEEKLRILPILIEDLTLPPG
                     ISHLKYANFLEWNHSERFFQAFNELLQALGIKKISVQAGGLSFSIANLRLLLVAEGTV
                     RGLSAWIEGFLDGFYEGSRAVRFIDRLQSVVCNEIERQDLERQLSDLANVLLPMEGDL
                     MESIKNDIELISCRIRKMKWAPKGDVFSPEENDGAASRIAWDLKHDLRRLAGSLKELI
                     REAAMLRSKEAVPVHSSSLGEPQ"
     gene            14502..15014
                     /locus_tag="VEL74_00700"
     CDS             14502..15014
                     /locus_tag="VEL74_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61071.1"
                     /translation="MRSFTHSTSAPHPNVFTTEFLARLGERDEPPTAGEADVAGPWRV
                     EAIPGHGFGLFRLGETLARGFAPVAIYPSRWLALVMAAILPGTGRDPLLRIEKTPNAD
                     GVYPLVLPSRDGAEQVGELQLFDEAAVDAMNVVIHLLQAPESLAFVLEAAGAVALERC
                     GAILDERTSE"
     gene            complement(15250..16158)
                     /locus_tag="VEL74_00705"
     CDS             complement(15250..16158)
                     /locus_tag="VEL74_00705"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYG61072.1"
                     /translation="MSRRMKSLLPVAVLALALAGLTPACGHAQSDDERALRRLKEVDW
                     PKAYKEQDVALLGRILADEFQMVDADGNKSTKAQELDWVRNNKPGYDSLTFEITRLDI
                     FENGTAIVGGTGTIRDEGSVTEYQSSNVLVKRDGTWRAVASHVSGVKPKQPEASAVQA
                     DSSTERDVAILREKIRKSGEAFNNVDPDAIIAPYARDVVLSYPGVPDMGYETLAKVYA
                     GLRDRKNVVEKTSPTIEEILVSGDLGIIRVMWDTTTTEVATGRQSSRQMKDLQVWRRE
                     RDGTWMFIRGMHFRIPPPAVPEKPAS"
     gene            16291..17754
                     /locus_tag="VEL74_00710"
     CDS             16291..17754
                     /locus_tag="VEL74_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HYG61073.1"
                     /translation="MTFVSELEPTPLWKHFDAILATPRPSKKEEAMRRYVLDLARRKG
                     FNVREDATGNLVVEKPASPGREGAPVVVLQGHLDMVTEKNAGVAHDFERDPIVPRRED
                     GWVKATDTTLGADNGIGVAAILAILEADDLVHGPLELLFTVDEETGLTGVVDLDANAI
                     ALRGRRLLNLDSEEEGAVTIGCAGGALTKMILQAETATVPVGSEAIEVRLAGLKGGHS
                     GMEIHLQRGNAVQLLARALHAASLDGPVHLASFQGGNKHNALAREAMARVVLPAGGRA
                     AFTAAMEREAAAIRDEFRAVEPDLRLEVSGGAAPEKVWSEAFGRKLLLLLTSLPHGVL
                     AMSQDIPGLVETSVNLAAVSEDNGEVHVLLSVRSSVASALLASRYRLRAFAEMAGARV
                     EENPGYPAWKPNVGSPLLGVFREVHKRVQGGDPELKAIHAGLECGVLGDKFPGMDMIS
                     FGPVIEGAHSPDERVHVESVGRFYRLLTELLEELARG"
     gene            17857..18222
                     /locus_tag="VEL74_00715"
     CDS             17857..18222
                     /locus_tag="VEL74_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61074.1"
                     /translation="MSKTTLYKSIFCAALLLALNIAPALALPAAGKPAPRQQQTAEHE
                     QNAQHEMSDAAKIAFTQAWSARKAAEAGKPANSSRKPVTQKAKPVSCEESWFFNWTKC
                     CSEWGCCTWYGDGNGPVCI"
     gene            complement(18300..19172)
                     /locus_tag="VEL74_00720"
     CDS             complement(18300..19172)
                     /locus_tag="VEL74_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYG61075.1"
                     /translation="MLVLAMLLVTAGSASAGAMEVGETMSANPSKPLESGHAPVNGIQ
                     MYYEVHGRRDGVPLVLLHGGGSTIDVTFSKVLPVFAGSRRVIAVEEQGHGRTTDRDAP
                     VTFEASADDVAALLRHLKVDKADLFGFSNGASIALQVAIRHPQLVRKQVFASAMTRKD
                     GAQPQLWEFMKQADFSNMPQPLKDAFLRVNPDAEKLKTMHDKDAARMRNFKDVPDELV
                     RSVRAPTLILVGDQDVVKPEHAVELTRLIPAARLLILPGGHGDYLGEAVMTQKETHYP
                     ELTARLIEEFLDSP"
     gene            complement(19199..19627)
                     /locus_tag="VEL74_00725"
     CDS             complement(19199..19627)
                     /locus_tag="VEL74_00725"
                     /inference="COORDINATES: protein motif:HMM:NF025471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4279 domain-containing protein"
                     /protein_id="HYG61076.1"
                     /translation="MDAEESTEDYEYYFAFSASLRVLDAPERHEEIERMTGLRPSHVH
                     LRGDLANEKSGRRWTRDVWGLKSPLSKETSMTEHLFWLWEQVKPHQAYFESLIAAGVR
                     VDVFCGYRSNCGYAGFDLNPEAFEIVRALRLPFEISVIVT"
     gene            complement(19633..20226)
                     /locus_tag="VEL74_00730"
     CDS             complement(19633..20226)
                     /locus_tag="VEL74_00730"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HYG61077.1"
                     /translation="MTLSPFPSESHMRWRRLDVPGREDARVERSPDGWCLTGQLDVEE
                     EGVPARLRYCIECDPEWRTRSALIEGEMGGRPVRFALAADGEGHWTRDGQALPGLTGA
                     LDVDLGFTPATNTLPIRRLSLVVGETAPVRSAWLRFPELRLEPLEQMYTREAERVYRY
                     KAQVDGDLFTARLDTDAYGRVLCYEGLWEAELAVSEE"
     gene            complement(20290..21288)
                     /locus_tag="VEL74_00735"
     CDS             complement(20290..21288)
                     /locus_tag="VEL74_00735"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYG61078.1"
                     /translation="MPGVSAILPVFNGRRYLRDAVRSVAAQTLPPCELIVVDDGSTDG
                     SLAELEGLPPAPFPIRVLRQENAGQSAARNLAARQAEGEYLAFLDQDDQWHPQHLEQL
                     IPPLEDDPAVGWVYCDFDEMDFDGHLVTRAFLRAAGMRHPKRTIFECVAGDLMVLPSA
                     SIVRRAAFEQAGGFDEELSGYEDDDLFVRFFRLGWEHVFVDRPLLRFRIHSTGSSASR
                     RFIDSRMRYAAKLAAMLPDDTRMFRYYVPDAIAPRFFFTSLDDYVRACSARDWEGARR
                     ALDAVNHFGRLRRRGLRLRWKLLWTQHPRLFRLLVALNDRLPYRARFVRNPTVTLR"
     gene            complement(21288..>22225)
                     /locus_tag="VEL74_00740"
     CDS             complement(21288..>22225)
                     /locus_tag="VEL74_00740"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG61079.1"
                     /translation="YEHRVRALRVESAGRSARVEAFTDRPDLLNHYPEHEHVRHGGFA
                     VYLACPPGHPVVLTLETDGGAASFPLRLPEGPLPPWPRSDDFDLGESPLLRRLAELVN
                     ARGGRDSRLLQIGARTPSGTEPIPRRHLLQGRVIGLDIHPGLKVDVVGDAHRLSTFLR
                     AGSVDAVLSASVLEHLQAPWLLAAEINRVLKPGGLVYHQAPGAWPAHAQPNDFWRISA
                     EGLRVLFGPENGFEILDARDSGPAAIIPGPEWREGFLDMPTVPAYATSEILARKVAEV
                     QPGAVAWPLATGESETRSRQYPVAGLRPVSPGGSP"
BASE COUNT         3776 a         7913 c         7233 g         3303 t
ORIGIN      
        1 gaaggagaag gccgcgggga ccagatgggc cggcagcgac tcctggagga aggccgacag
       61 cgccggcgcg tccacccgct cgtcctggcg gatcacgaac gccaccagcc gcctctctcc
      121 cccttcccca cgggccacca ccaccgcctc gccgacgccg ggatgggtca ccagggcggc
      181 ctcgatctcg ccgggttcga cgcggaagcc ccgcaccttg acctggtgat cgatgcgccc
      241 gaggaactcg agccggccgt ccggcaggaa gcgggccagg tcgccggtgc ggtagagccg
      301 ctcgcccgga gtaccggcga aagcgtcggg catccagcgc tcggcggtca gctccggccg
      361 ccccaggtag ccccgggcga ggcccgcgcc gcccaggcac agctctcccg cgacgccggg
      421 aggcaccggc tgcagcttgc ggtccaggac ccgcgcccgc gtccccgcca gggggcggcc
      481 gatggtgggg atctcacccc tctccacgag ggagaaggtc gagtaggtgg tgtcctccga
      541 cggtccatag aggttgaaga cccgctccac ctggggcagc tcgtggaggc gcgcggccag
      601 ctcgcgcgtc agcggttcgc cggccaggtt gacggtgcgg accgaggccg gcagccccga
      661 ggtgcggagc agcgtcgaca gcgccgaggg gacggtgttg accagcgtca cctcgtccgc
      721 ggcgggcagc tccggcaggg ccaggacgtt ctcggccagg accaccgcgc cgccccggga
      781 gagggggacg aacagctcga agaccgagag gtcgaaaccg atggaggtgg aggcgagcac
      841 ccggtgcagg tccgcctctg ggaagacctc ccaagcccac cgcaccagag ccaccgcgct
      901 cgagtggcgg atcgccaccc ccttgggctc accggtggag ccggaggtgt agatgaggta
      961 ggcgagggtg tcgctggcga tcagcctctg tgtaggggag gggcttgccc cctcccggga
     1021 gggggcaagc ccctccccta catcggagct cagggccaac cgttgggccc catgctccgg
     1081 caaaatctct tccagcccct cccccaccag caccgccgcc ggccgggcgt cggccaggat
     1141 gcgggccagg cgcggccggg ggtgggccgg gtcgagaggc acgtaggcgc cgcccgcctg
     1201 gaggatgccc aggaggcccg ccaccagctc gggtgaccgg cgggcacaga tgcccacccg
     1261 gtcctcgggg cccactccca gcgcgcgcag ccggtccgcc accgcctccg accgggccgc
     1321 cagatccgcg taggtccagc gatcctcgcc cgcgatgagc gccaccgtct gcggtgtacg
     1381 cgcggcctgg tccaggaaca gctcgtggag gaggcgatcc ggagcgggct cgatcgcggg
     1441 cccctccgcc cactcgacca gcacctgccg gcgctccgcc acggagagca gcggcagctc
     1501 ggagagcggc agggacggat cggcgaccgc cgcggccagc agcgtccgga agtgaccagc
     1561 gatccgagcc gccatggccg gatcgaagag gtccagggcg tactggagcg aggccaccag
     1621 gccgtggctc ccggcctcct ccgccaccat cagggtgagg tcgaacggta cccgccgctc
     1681 ggcgagccgc accgactcgg ccgccagatc cgccagcgcg gaccgcgccc cctcctcgcc
     1741 cagcgcgaag gcgctccagt cccggtccgc cggccggtgt gcccgctgca gcacgagcat
     1801 cgtctggaag agcggcgacc ggccggggtc gcgctccgga tgcagccgct ccgccagcag
     1861 cgtcagcggc acaggattcg ccagcgctcc cgacaccacc ccggcggtcc gtccgaggag
     1921 atccccgaag gccgaatccc ccccgaggtc ggcgcgcaag ggcagggtgt cgatgaagta
     1981 gcccaccacc cccgccaggt ccgccgacgt ccggccggcc gatggcgcgc cgacgatcag
     2041 atcctcctgg ccggtcaggc gatggagcag ggtctggaag ccggcgagga gagcggcgaa
     2101 cagcgtcgcg ccgcgacggc gggccagcgc cgccagcccg tccgacagct ccacgcccag
     2161 cggcagcacg gccgagccgc cgcgccaggt gggcagcgcc ggccgcggcc ggtcccccgg
     2221 caggtcgagc gccggcagat cgccgcccag ccgctcaccc cagtacgccc agagtgcctc
     2281 ggctccggaa gccagccgct cccgctcgcg ggccacgtga tcggcgtacg tccgggagac
     2341 ggccgcgagc ctggcctccc ctccctcgat ctcccgcctg tagagcgcct ccagctcccg
     2401 cgccagcacg gcgagggacc agaagtcggc gatcaggtga tggatcgtga ggagcaggac
     2461 gtgctcctgc tccccccgga cgaagagagc cacgcgcagc agcggcccgc gcccgaggtc
     2521 gaacggccgc cacgcctccc gggccagcca ctcccccagc tccgcctcgc tccaccgcgc
     2581 cgcatcctta tagaagaagg tctccggctc cagcctccca tggacccgct gcaccggctc
     2641 gcctccctgc tcttcgaacg tagtccgcag agccggatgg cgaaccgcca tcttctcgac
     2701 ggcccggaag agagccgccg cgtccagatc cccccgcagc cgcagcggca ccgggatgtt
     2761 gtacgccgaa gccgctggag cccaccgctc caggaaccac agcccccgtt gcccatggga
     2821 cagggggaag acatcttgct gattttcccc tctcccggtc ggggggaggg gatgggagag
     2881 ggggacaggg ggtgaggggt tcaatccctc gagcaccgcc gccagcgacg gcgcctccag
     2941 ccaccgagtc agcgggatcg acacccccaa cgccgtttcg atccggtggc tcaactcgac
     3001 cgcggccagc gagtcgagcc cgagctccac cggcggtcgg ccggggtcca ccgccgccgg
     3061 gtccaccccg gccgcccgcg ccagctcggc gcgcaggaac gcctcccggt cgggcagcgc
     3121 cccttcctgg agatcctcgc cgtccaccgc cgccgccgcg tcccgcccga ccacctccag
     3181 atccccagcg agatacccgg cgcggcaggc gtgccgctgg accttgccgc tggacgtccg
     3241 gggcagcgtg ccggtgcgga tcagcaccac ctcatggatc tgcgcctcca gctcctccgc
     3301 caccacccgc cggaccgcgg cggccaccgt ggcgacggtc tccggctccg gccggaaccg
     3361 ccgggcgatc tcgtggacga ccaccagccg ctcctcgccg tcgacctcca ccgagaacgc
     3421 cgctccacct ccgggctcca gccccggatg gctcgcctcc gccgccagct ccacgtcctg
     3481 gggatagagg ttgcggccgc gcaggatgat caggtccttg agccgcccgg tgacgaacag
     3541 ctcgccgtcc cagaagaagc cgaggtcgcc ggtgcgcagg aaccggtcgc ctccagccgg
     3601 gctgccggcc aggacggcgc cgaacgtccg cgccgtctcc tcggggcggt cccaatagcc
     3661 gccggccacg ctcggaccgg ccacccagac ctcacccacc cggcccggcg gcaccgccgc
     3721 gcccgtctcc gggtcggcga ccacgatccg ctggccgtcc caggcccggc cgcagcccac
     3781 cagcgcccgg gcgttccagc tctcctccgg cagcgcggga accgcgctcc cctgctccag
     3841 ggccgtgggg tcgaaatacc ccctcgccgg caacacgccc cgctgcccgc cggagacgaa
     3901 cagcgtcgcc tcggccaggc cgtagcaggg atagaaggcc tcgcgccgga agccgcacgt
     3961 ggcgaaggtc tcggcgaagc gctccagcgt ggcggcgcgc accggctcgg cgccgttgaa
     4021 ggccacggac caggacgaga ggtcgagcgc cgcccggtcc tcgggcgtca ccttgcgcac
     4081 gcagaggtca taggcgaagt tgggcccgcc gctggtcgtc gcgcggtagc ggctgatggc
     4141 ctcgagccac cgggccggcc gctgcaggaa cgccatcggc ggcatgagga ccgcggacga
     4201 tccggtgcag agcggctgca ggacgttccc gatcagcccc atgtcgtggt acagggggag
     4261 ccaccccacc accaccgact cctccgactg gccgaaggcg gcgcggatca tctcctcgtt
     4321 gtggaggatg ttgccgtggg tgacgatcac ccccttgggc agcgcggtgg agcccgaggt
     4381 gtactggagg aaggccggcg tctccggctt cgtctcctcc atgagctgct cgtcccactc
     4441 ctcgccgagc cgcggctcca gcgccgccgg gaccagccag gacgccgccg ccagctcggg
     4501 ggtcggttcg gcgatcccct cgcgccgcgc cgccagctcc ggcgtggtca gcacgaagcg
     4561 cggccgggcg tcccgggcga tggcgcgcag ccgcgggtgg tcccgccgcg gacggggtgg
     4621 atagcagggg accgccacga cgccggcata gagacagccg aagaaggcgg cgatgaagtc
     4681 gagccccggc gggaacagga gcagcgcccg ctcaccctgg ccgcgctccc gggcgagcgc
     4741 cgcgccgatc gcccgggcgc gggcgtccag ctcggcccag gtccaggact ccgccccgtc
     4801 cgcgccggcg gcaccgtcgg ccaggaacgt gtaggcccgc cggcccggcc gctcgagagc
     4861 gtgggcgcgc aggacctcca ccagattcga gcgcgcgccc gaacgcggag ccgatgactt
     4921 gatcagccgc gtgctcacgc gcggctcccc tgatacatct cgtccatccg aacctctccg
     4981 gaagcttgtc tgcggtgtga cctctggggt gtactgcaga ggcggggggc gaagactacc
     5041 atgtccgttc cggctccccc ctcagtttca aggtgccgca ggtcacccca agcggccctt
     5101 ccacgtcttc cgtccatgaa acgagataga ctgtccgagt acctcacctc gaccgccatg
     5161 gactcacgca ccgagagccg tctcctgcgg atggccatct ccaggggatt cctccagtgg
     5221 gaagacctgg acgcggtcgc cgaccgcctg cccgaccgcc aggacgggcg ggacagcacc
     5281 cagccgggcg cgcgctgggt ccacgctctg ctcgacgccg gccgcctcga cccccgggtg
     5341 ctggcggacc tctccgccga actcgagcgg ggacgcgacg acctgacccc ggagctcacc
     5401 gcccgcccgg tctccctcgg aacctccccg cggcagctcg agctccggac caccgagctc
     5461 cccctcaccg acagctcgtc gccgcatctg tcctcccgga cctcccgcat cgacccggcg
     5521 acgctgccgg tcgagctccg cttcctctcc tcctgggagc gctaccgcgt cgagcgcttc
     5581 ctcggcgccg gcggcatggg cacggtctac cgcgccttcg accccttcct cgaccgctgg
     5641 gtcgcgttga agttcctgca ctggaacgaa acctcccagg tgggccgctt catcaacgag
     5701 gcgcgctccc aggcgcgggt ggaccacccc aacgtctgcc gcgtctacga ggtcggcgag
     5761 gtcgaggagc gcccctacat cgccatgcag tacatcgagg ggcggagcct ctcggacgtc
     5821 cgcgacgagc tgccggccga ggccaaggtg cggctggtgc gagacgtcgc ccgcgccgtc
     5881 cacgccgccc accggaacgg tctgatccac cgcgacctca aaccaggcaa catcctggtg
     5941 ggaacggatg attccggcgc cctccacccc ttcgtcgtgg acttcggcct cgcccgcgac
     6001 caggacgagg acggcctcac ccgcagcggc atgctgagcg gcaccccggc ctacctctcc
     6061 cccgagcagg cccaggggct gccgctcgac cggcgcaccg acgtctacag cctcggcgtg
     6121 gtgctctacg aaactctggg gggacgcccc ccgttcctcg ccgccaacgc cgccggcatc
     6181 ctggtccgcc tcgtccagga ggaggcggag ccgttgcgca agctccagcc ccaggtgccc
     6241 ccggacctcg agacggtggt catgaagtgc ctggagaagg acccggcgca acgctacgac
     6301 tcggcgcggg ccctggccga ggatctcgac cgctatctgg agggcgagcc ggtgcacgcc
     6361 cgtcccgcga gctgggccta ccggctgggc aagaaggcgc gcaagaacaa ggccctgacc
     6421 gccgtcgccg ccgccgccac gatcgccctg ctcgtcttgg gaggcgtggc cctgcgcgcc
     6481 gaatggcagg cgcgggagcg ggccgccctc gcccagggct tcggccagcg ggtcaaagac
     6541 ctcgagtacc gcatccgcat cgaggccttc ctggcgccgc acgacatccg cgcccacaag
     6601 cagcaggtcc gccgcgaggc cgaggcgatc cgcgccgaga tggcgcgcct ggggccgctc
     6661 gccgccggac ccggccacta cgcgctgggc aagggctacc tggcgctcca cgagtacgag
     6721 acggccctcg atcatctgga gcgggcctgg caggcggggc agcgcactcc ggaggtggct
     6781 tcggccctcg gccgcaccct ccaggtcctc taccagaagg ccctctggga cgccgcccgc
     6841 gagcagaacg tggacgcccg ccgggccgcg cgcgaggaga gcgagcggac gttcagccgc
     6901 cccgccctct cctacctgcg ggagatcccc gcagaggaga cgagccggca gccctacctc
     6961 gccgcgctcc gcgccttctt cgaggagcgc taccccgagg ctctggccaa ggcgcgcgag
     7021 gcctaccggc gggacgtcgc gttctacgag gcggcccagc tcgaggccga cagcctgatg
     7081 gcccagggaa accaggaggg ctccgctggc cggtacgaag aggcgctccg cttctatgac
     7141 caggccggcg agaccttccg cgccctgctc gccaacgtgc gcagcgacgc cgccctctac
     7201 gccgcggact gcaaccgccg catcctccgg ctcgacgccc ggatggcggt cggcggcctg
     7261 tccgaggccg aggtgctgga tgccgcggcg gcctgcgacc gggccctcca ggtggacccc
     7321 gagctcggcg aggcgttcac cagcaaggcc cgcctctatc agcgctgggg gcagcaccag
     7381 ctccagagcg gcgaggaccc gacgccggcg ctgtcggacg cccgcgccat ggcggagcgg
     7441 gccatcgccc tgaaccccca ggatgcccag gcctactccc acatggcggt ggcgatgcgc
     7501 atcctcgccg cctgggagca gagccatggc cgcgcccccg gctcccacct ggaacaggcc
     7561 gtccaggccg cggccaaggc ggtggagctg cagccccagc gggccacctt ccgcaacaac
     7621 ctcgcgacga tctatctgaa gctcgccgag gtccagatgg ggagcggcct cgacgccggg
     7681 gcggccatcc gcagcggcat cgccaacgcc gatcaggcga cgcggctgga cacccgtttc
     7741 ggcctcgcct acatcaatct ggggagcgcc tggaagacca tggcggagta ccagatctcc
     7801 cgcggcgacg atccgacgga atcgacctcc cgcggcgtcg aggccttcga gtacgccatc
     7861 cgtctcaacc ccaactcggc gccggcctat aacaatctgg gcaacgccca tctcaccctg
     7921 ggcgactatc agatggcgcg cggcgcggcg gcgcgcccct ccctggagcg cgccatcgcg
     7981 agctatcgca aagcggtcga ggtccggccg gactacgaag tcgcgatctt caacctcgcc
     8041 tggacccaga ggctcctcgc cctggaccag ctccagcgcg gcgaggaccc cgccccgagc
     8101 ctcgccgccg cccgcgagac cctcggccgc atcgccaccg acgccgacgt cctgctggag
     8161 cgcgcccgct gtgacgtcat cgccgcccgc tgggccatgc gccagggccg cgaatccggc
     8221 cccctcctgc gccaggcctc ctccactctg gagcaggcgc gggacctcaa cccgcagcaa
     8281 gccgacatct atatggaagg cgccctggcc gcccgctacc aggccgagca ggatctaaaa
     8341 aaaggcggcc gccccgccgc cgccgtgcgc gacggcctca cccgcatcga gaaagccctg
     8401 gagatcaacg cccaggaagc gcgctacctc gccgagcagg gagtcctcca gaccctcgcc
     8461 gcccgcctcg agaccgaccc cgcgaggcgc aggcaggaag agagccgcgc cgccgccacc
     8521 ctgggcaagg ccctcgaggc caatcccctg ctgcgaaggg agtatgggcc gtggctagag
     8581 ggtgggcgta tttgaaatca tccttctggc atgtcaatcg caccctggcg aggcgtcagg
     8641 cgaactggct actgtggcac gaggtggggc ggcgtggggc gggagctgga ggccggtctt
     8701 ctcaaagtct tgtcctttct tccggcccca cacgctccat gcaagcgagt atcgccggga
     8761 cgggggcgac ggtgctcagg aactctcccg ttcggatcgt cagcaccttc cagcctcgct
     8821 ccggcgcgaa gctctcccgc aactccgcct cggtcacctg ccggggaacg ttcggaacgg
     8881 ggaacgtcac ggcaaactcg tgcagataac accgcccgcc tggacgaagc acccgcgcca
     8941 gctcatccac gaaccgttcg cactgctcag gatcgaacag gtggtagaac ccggagtcga
     9001 cgacggctcc gaaggtctgc cccagaagag agggttgaag cgcgtcggcc actcggaagg
     9061 cgaggcggtc agcgacatca gcaggaaggg cggccatctt cgcacgcgcc tgggtgatag
     9121 cttcctcgat gaaatcaatg ccgagcgtat cgagcccgct tttggcgaga tgaatggcaa
     9181 gatctcccga gccacagccg acatccagcc cggggtcttc cggggggtac tccgcgagca
     9241 ggccggccat cgcaggctgg ggcccgccga tgtcccacgg cgccggtcct cggtacaccg
     9301 cttcgaaaaa ggagagggga tcaggtccga atcggctcat aagtgacgta tcgcctcgac
     9361 tctccctgac cagcctgcct cctcagacac ttcccgtcgc gagtagcgac tcgagccggt
     9421 cgaggttctg ctcgttggcg gtcgcgctga gggggcgcat cttcgcggcg acctcggggc
     9481 tctcgaactc ctggacccag gccaggtgtg tcttgtcgcc gcgagacgtc agtgtcaccg
     9541 tcaacctgaa ccacggctgt acgacgtgct cgagcacgat cctggtatct ggctgaatct
     9601 cccggaagac actctcgttg gggttgctgg caccattcgg gccgtgcatc gcaaagaccc
     9661 accggccgcc gggcttgaaa tcgaactgct cgaacgtgtt cgtgaagccg ctcggccccc
     9721 accattgagc gaggcggtcc ggttgctcga acgcggcgaa tacctgtcgc gggttggctg
     9781 acaataaccg ctcggtgctg acggcggcgt tcgggttgtt ctctcgggac atcggcagct
     9841 ccttcgtggc ccaaacgttt tgaggcgagc cgccgctgga ggccggcgga agcggccggc
     9901 ccgcgcgaat gtcaggcctc gcttctccgt gcggcagtcc cttgcttcat gcgagcaccg
     9961 atctgaattg ccaaccaggc cattggcagg taggccgcca gcaggtccag cgcgatgaac
    10021 caggctgggg cgggaatcat gaagctcgcc gccacaccac cgcaaaggaa gacgacgccc
    10081 atcgcgtaag cgatccgcgc cttgtagctc gccgcgatca ggtacgcagc gagcgcgcca
    10141 gcgagggtac cgactgcgtg cgccaggaac ggcatcacga agtgccgcgg ctcgaagagg
    10201 tgcatggctt ggctgaggct ctcggcgttg gccacatcca cgccggccgg cggaggaatg
    10261 agcgacgggc tgagagtgat gagcgccgag ttgacgattc cgccgatgac gattcccgcg
    10321 aggacggcaa gcacatttcg cagcaggctg gtcacgggcg attctcctga gaggcctaac
    10381 ctaggattcg atgctcggca gagtctagcc tcggcttgct ggcagcgtca aaccgataga
    10441 gccggcgcgc cggaggcacg agaaaggcgc tccgcctgct ggcacctacc ccaggaggcg
    10501 gaggactgct ttccgcctcc ctagtcggga ggccccctac gattcagttg tcaaagagca
    10561 aacggcaaag gctgccttcg cctccatctt accaatagta ccatcgcgtc gcccctgtag
    10621 tctactgagc ggagcgtgca tcgcgttctt cctctcgatc tcagatgcta gcggcggatc
    10681 cgtcagctct ttgacaacca aagggcaccc ccccgagcgc ggggccgggg cggggcgcct
    10741 tacgctcgcg gggcatggca ggaccgcaat gccgcgcgga ggagcgactc ggctccacgc
    10801 cgcaccggcc cgggctcctc caccgccggc tatccggtcc gcggcgacga tccagggatc
    10861 actacggatc acaaaccagg gtgaaacgac cgccggggaa gccggggaga tcattctccc
    10921 ctcaggggga gtgatccggg gagcgcgggg aatcactcta cccgggctcc ggaacacaaa
    10981 ccgggctccc ggaggggtcc gggaacacgg gggaatgatc cacgcgagtg gagggatcat
    11041 tccccgagca ggaagagtga ctcgggcccg ggggagaatg gtgcacccgc gagggattgt
    11101 gatcccggaa ggcccggttg tgttccagga gtgctccgtt gtgacccggg aacactccct
    11161 tgtgaccctc tcaggcgtgg gcgaacgcgc cggccatctg cgcgcaggac tccgcgcagc
    11221 gctgacaggc ctcggcgcac tgctgcatct ccgggtcgct gcccagccgc cggcagtcgt
    11281 cggcgcagcg ctggcagacc tcggagcagg cgcggcaggt ctcggtgtgc agctcggagc
    11341 ctcggatcat gaagttggcg ctggtctcac agatctcggc acagtcgagc agcagccgga
    11401 tgtgagacgg ctcggcatgc gggccgccct tctccaggca atgcgcgaca ttctgcaggc
    11461 agaccccgtg acactcctgg cagttctcga tacagcgctc catctcctgg gtttgatgtg
    11521 gcatggatgc acctccttgg ggttggactg cttcaccaca gtcggagtgg ggacctgcgg
    11581 aggcgggctc gcgcggcggg cccgtttccg tcgaatctca gatcggaaag gcgtgcagag
    11641 agcttgccag cggcgcggtc cgtcgccccc agtcaggcgc gaagacccgc atccagcatg
    11701 gaatagagcg cctccagccg actacggccg gggccgcgag caggccaccg ccccacgact
    11761 tcgcgaacct ccggcgaggc atgctcgacc gcccaccgga tctcctcgtc gccgacgccg
    11821 gtatcgccgt ccgccgcgat ctctcgggca cgcgccgcgt acacggcagg tcctaaaata
    11881 tgcttcgcct ggtcgagggt cggcaacggg tgggtataag cgaccgccgc cgccaggccc
    11941 gcagcgcgag cggcggccgt ggcagccgga tcgccaacct cgcgtgcggc cgcgtgagcc
    12001 gccaagccag cgagcgtagc ttcgccgttc gcctctcccc acgcgcgaag atccggatgc
    12061 cggcgatggc ctcgcgtgga cgtgtgtcgg caggagcctt cacctcaaac agtggcagta
    12121 cccgctcggc acaatcggca gcccagaatc caacaagccg gaggtcgtcc tggctcaaca
    12181 tcattctacg tagcccgcga tccgtggcgt ccggtgcagc gctcaggcct ggcatctacc
    12241 ccatctttgc aggtaaattt ccatcaacag gagtagccga caaaatgaat gggtagtcct
    12301 ctgttccgac ctccttgaat aaacgcaaga tgagctcaag cggctcatcg gggaaggtgg
    12361 acacctcaaa cagtaggtcc aggacgacac tttcccagcc gccggggaaa attgacgaaa
    12421 gtcttggcaa attgcaaacc gacactccat taggctgcat gacacaggat cttgcacccc
    12481 tggctccaat aactgcggaa ggcaccacga cggccaagtc aaacgtttca ccatcgacca
    12541 tcctagtcgc agggttatgc atgccaatcg tcagcctcaa ttcggagggc ctccctcgcc
    12601 aaaccatgac tggggcagtg actagtggtc tcccagccag tggtactcca tttgcaaaga
    12661 aatcgaaacc ccaatccacc ttagcaagag actgcttcgg attctcaatg caccaacgca
    12721 ctctagcctc caaccgcgac ttcagcacag caatcttgcc accataaact atatgagggt
    12781 agtgctttaa gtcgaagggg atgtcatcag aactctgcgt aagcagaatg acctacttat
    12841 taagcgcatg agcataacca acctcataga agacattggg gtttcgcccc gtcatctctg
    12901 caatgatgac atccgccttt gcaatttgat tgtagaccct cgccaagata ctctcaacaa
    12961 atatctgctc gtcgactctc tcgcagtatg cgcctgcatc cttacaggcc ggtttgattc
    13021 cagcctcgta tacatcatca aagctctcat caaaaggcat caacacgaaa acaaacgatt
    13081 taggcttagt atcttccatg agctattccg cttgcctatt gagctactgc ggctcgccca
    13141 acgaacttga gtggactggc accgcctcct tgctcctcag catcgcagct tccctaatca
    13201 actccttcag ggagccagca aggcgcctaa ggtcgtgctt gagatcccat gcaattctag
    13261 atgcggctcc atcgttctcc tccggactaa agacatctcc tttcggggcc cacttcatct
    13321 tccttatgcg gcaagagatc aactcaatgt cattctttat gctctccatt aagtccccct
    13381 ccatcggcag cagcacgtta gctagatcag agagctgtcg ctccagatct tgcctttcaa
    13441 tctcgttgca gacgacagac tgaagtcggt ctatgaatct tacagctcta gaaccctcgt
    13501 aaaagccgtc taaaaagccc tcaatccaag cactcagacc tcgcacagta ccctcagcaa
    13561 cgagcaatag gcgaagattg gcaattgaaa aactaaggcc acccgcttgg acggagatct
    13621 tcttaatccc gagcgcctgc aggagctcat tgaaagcctg gaaaaagcgt tcactatgat
    13681 tccattccag gaaattggca tacttcagat gtgatatacc tggcggcagc gtcaaatctt
    13741 cgattagaat cggcaggatt cttagcttct cctcccccat agctcggaat ttggcaatac
    13801 taagctcctc ttgcacccac ctagatttga gagaatgtga ggatatgata taagcgagat
    13861 gtgtcgcttc tgatataccg cgctcaatgg acaacctgat atcatctcct actcggatgt
    13921 ctcgatcgtc aagaaacgtc tccaccccct caaggctcaa agcctcataa agacgtacaa
    13981 caaacttctt atccctactg gaatgcgaca ggaaaacttt catggcagcc ccaaagtgac
    14041 ggatgttccc ccggcttcca tacggccata tagatggcct ataaccgcat ggaggatcca
    14101 agttacttta agcaatcctc ctcacataga aagcacttac ggaagtctag ccccgcgaag
    14161 cgctggtgtc aaaaggatag agagctgacg atacggaggt gttcgggtac ccggctagcg
    14221 gaggaggagt ggctgcggcg cccccccgcg aggctcaggc acgaaagagg ctttccgccc
    14281 gcttgacacc cgctcccact ccgcctacaa tacagacact ggaacacccc gagagaaaac
    14341 cacgatcccc ttagccgttc caaagatcgt gggctccgga gagttccggc agttggctgt
    14401 aggcgcgcaa gcgtgcggcg gcaatgatgc cgaagcggcc cggaggtgag ctggtacctc
    14461 gacccccgag cctgaccgca cataccccga taggagggac tatgcgcagc ttcacccata
    14521 gtacatccgc cccccacccc aacgtcttca ccaccgaatt cctcgcccgc ctcggcgaac
    14581 gggacgagcc gcccacggcc ggcgaggccg atgtcgccgg gccgtggcgg gtcgaagcca
    14641 tcccgggaca tggcttcggc ctgttccggc tgggcgagac cctggcccgc ggcttcgccc
    14701 cggtggcaat ctacccctcc cgctggctgg ccctcgtcat ggcggccatc ctccccggca
    14761 ccggccggga cccgctgctc cgcatcgaga agacgcccaa cgccgacggg gtctatcccc
    14821 tcgtcctccc cagccgggac ggcgcggagc aggtcggcga gctccagctc ttcgacgagg
    14881 cggcggtcga cgccatgaat gtggtgatcc accttctcca ggcgccggag tccctggcgt
    14941 tcgtactgga ggcggccggc gccgtcgccc tcgagcgctg cggcgccatc ctcgacgagc
    15001 ggacgtccga gtagccctgc cctgaggcgc ccccaggctg ggggcgcctc gccctacctc
    15061 ctgctccacc ccatcatcaa cccagtcatc caacgagcca ttatggtagg atgttgctcg
    15121 cgaggagtgg ccctgttgca ccgtgaaggt cgattcctcc ggctctttga caactgacgt
    15181 acacgtccag agcgcggccg ccgtgaggcg gagcggcggg ccgcgctcct cagacgtggt
    15241 gtgccctccc tacgacgccg gcttctccgg caccgccggt ggagggatcc ggaagtgcat
    15301 gccgcggatg aacatccagg tgccgtccct ctcccggcgc cagacctgga ggtccttcat
    15361 ctgccgggac gactggcggc ccgtggcgac ctccgtcgtg gtcgtgtccc acatcacccg
    15421 gatgatcccc aggtcgccgg agaccaggat ctcctcgatg gtcggcgagg tcttctccac
    15481 gacgttcttg cggtcacgca gcccggcgta gaccttggcg agcgtctcgt agcccatgtc
    15541 cggcacgccg ggatagctga ggacgacatc gcgggcatac ggggcgatga tcgcgtcggg
    15601 atccacgttg ttgaaggctt cgcccgactt gcggatcttc tcgcgcagga tggcaacgtc
    15661 ccgctccgtc gacgaatcgg cctgtacggc gctcgcctcc ggctgcttcg gcttgacgcc
    15721 cgagacgtgc gaggccacgg ccctccacgt tccatcgcgc ttcaccagca cgttgctgga
    15781 ctggtactcg gtgacggagc cctcgtcgcg gatggtgccg gtgccgccca cgatggcggt
    15841 gccgttctcg aagatgtcca gccgggtaat ctcgaaggtg agcgagtcat aacccggctt
    15901 gttgttgcgg acccagtcga gctcctgggc cttggtgctc ttgttgccgt ccgcgtcgac
    15961 catctggaac tcgtcggcga ggatgcggcc gagcagcgcg acgtcctgct ccttgtaggc
    16021 cttcggccag tccacctcct tcagccgccg cagggccctc tcgtcgtcgg attgcgcgtg
    16081 gccgcaggcc ggggtcagcc ccgccagggc gagcgcgagg acggcaaccg gcagcaggct
    16141 cttcatccgt cgtgacatgg aaacctcctc tggatggctg ctcttatagc agccccggcg
    16201 ccgggggcgc tcacggccat tcgggatttt tcacattctt tacagcggct ccgttgtatc
    16261 cttcgcccca tcgaccatgg aggccgcctc atgacctttg tctcggaact ggaaccgaca
    16321 ccgctctgga agcacttcga cgccatcctc gccacgcctc gcccctcgaa gaaggaagag
    16381 gcgatgcggc gctacgtcct cgacctggcc cggcgcaaag gcttcaacgt ccgcgaggac
    16441 gccaccggca acctggtggt cgagaaaccg gcctctcccg gccgggaggg ggcgccggtc
    16501 gtcgtgctcc aggggcacct cgacatggtg acggagaaga acgccggggt cgcccacgac
    16561 ttcgagcgcg accccatcgt cccccggcgg gaggacggct gggtcaaggc cacggacacg
    16621 acgctcgggg cggacaacgg catcggcgtc gccgccatcc tcgccatcct ggaggcggac
    16681 gacctggtcc acggcccgct ggagctgctg ttcacggtgg acgaggagac cggcctcacc
    16741 ggggtggtcg acctcgacgc aaacgccatc gcgctgcgcg ggcggcggct gctcaacctg
    16801 gactccgagg aggagggggc ggtgaccatc ggctgcgccg gcggcgcgct caccaagatg
    16861 atcctccagg ccgagaccgc gacggtcccc gtaggctccg aagcgatcga ggtgcggctc
    16921 gccgggctca aggggggcca ctcgggcatg gagatccacc tccagcgcgg caacgccgtc
    16981 cagctcctgg cccgcgccct tcacgccgct tccctggacg gcccggtcca cctcgcttcg
    17041 tttcaggggg gcaacaagca caacgctctg gcccgcgagg ccatggcccg ggtggtgctg
    17101 cccgccggcg ggcgcgccgc cttcaccgcg gccatggagc gggaggccgc cgccatccgc
    17161 gacgaattcc gtgccgtcga gccggacctc cggctcgagg tctccggcgg cgccgccccc
    17221 gagaaggtct ggagcgaggc tttcggacgg aagctcctgc tcctcctcac ctccctgcct
    17281 cacggcgtgc tggcgatgag ccaggacatc ccgggactgg tcgagacgag cgtcaacctc
    17341 gccgccgtct ccgaagacaa cggtgaggtg cacgtcctgc tgagcgtccg cagctccgtc
    17401 gcctccgccc tgctcgccag ccgctaccgg ctgcgcgcct tcgccgagat ggccggagcg
    17461 cgcgtcgagg agaacccggg ctatcccgcc tggaagccga atgtgggctc tcccctgctc
    17521 ggagtgttcc gcgaggtcca caagcgggtg caggggggtg accccgagct gaaggcgatc
    17581 cacgccggcc tcgagtgcgg tgtcctgggt gacaagttcc ccggcatgga catgatctcc
    17641 ttcggcccgg tgatcgaagg cgcccactcc ccggacgagc gggtgcacgt cgagtcggtg
    17701 ggacgcttct atcggctgct caccgagttg ctcgaagagc tggcgcgcgg ctgaggggaa
    17761 cggcggccgg gaatcgtttc agagcgcagt gaccaccgag gctgatcctc gaagggtttt
    17821 gaaaataaaa ctgactctga aacggagacc tgaaaaatgt ccaagacgac tctctataag
    17881 tcgattttct gtgccgccct gctcctcgcc ctcaacatcg cgcccgcgct ggctctgccc
    17941 gcggccggca agccggctcc ccggcagcag cagaccgccg agcacgagca gaacgcccag
    18001 cacgagatgt ccgacgctgc caagatcgcg ttcacccagg cgtggtcggc ccgcaaggcc
    18061 gcagaggccg ggaagccggc caactcgtcc cgtaagccgg tgacccagaa ggcgaagccg
    18121 gtttcctgcg aggagagctg gttcttcaac tggaccaagt gctgctccga gtggggttgc
    18181 tgcacctggt acggcgacgg caacggcccg gtctgcatct gagcgctcgc cgcccagagc
    18241 accgcaaggc ggagggcctg ccggccctcc gcctccaact cactcggcac gcgtcaatct
    18301 caaggggagt cgaggaactc ctcgatcagc cgcgccgtca gctcgggata gtgagtttcc
    18361 ttctgcgtca ttacggcttc gccgagatag tcgccgtgcc cgccgggcag gatcagcagg
    18421 cgcgcggccg gaatcaggcg ggtcagctcg acggcatgct cgggcttcac cacgtcctga
    18481 tctcccacaa ggatcagagt cggcgcgcgg accgagcgga ccagctcgtc cggtacgtcc
    18541 ttgaaattcc gcatcctggc cgcgtccttg tcgtgcatgg tcttgagctt ctccgcgtcc
    18601 gggttcaccc gcaggaaagc gtccttgagc ggctgcggca tgttcgagaa gtccgcctgc
    18661 ttcatgaact cccagagctg cggctgagca ccatctttcc tggtcatcgc tgacgcgaag
    18721 acctgctttc gtaccagttg cggatgccgg atggcgacct gaagagcgat gctggcgccg
    18781 ttgctgaacc cgaagagatc cgccttgtcg actttcaggt gacgaaggag ggccgcgacg
    18841 tcatccgcgg aggcctcgaa ggtgacgggc gcgtcgcggt ccgtcgtgcg gccatgcccc
    18901 tgctcctcca ccgcgatgac cctgcggctg ccggcgaaga ccgggagcac cttgctgaac
    18961 gtgacatcga tcgtggaccc gccgccgtga agcaggacga gcggaacgcc gtcacggcgg
    19021 ccatgcacct cgtaatacat ctggatgccg ttgaccggcg catggccgct ctcgagcggc
    19081 ttcgatgggt tggctgacat cgtctctccc acttccatgg cgccagcgga tgcgctgccg
    19141 gcggtcacca gcagcatcgc cagcacgagc acgaacgtac acctcatgga tgcattgctc
    19201 acgtgacgat gaccgagatt tcaaaaggca acctcagcgc ccgtacgatc tcgaatgctt
    19261 ccggattcag gtcgaaccct gcgtacccgc agttggagcg gtacccgcag aagacgtcga
    19321 ctcgaacgcc ggcggcgatc aacgattcga agtaggcttg atgtggcttc acctgttccc
    19381 acagccagaa caggtgctcg gtcatcgagg tctctttcga aagaggcgac ttgagccccc
    19441 atacgtccct ggtccaacgg cgccctgact tctcgttggc taggtcgcct cgaagatgaa
    19501 catgcgaagg ccggagcccg gtcatccgct cgatctcttc gtggcgttcg ggagcatcca
    19561 gcactctgag gctggcagag aaggcaaagt agtactcgta gtcttctgtt gactcttcgg
    19621 catccatgct ctctactcct ccgacaccgc gagctcggct tcccacagcc cttcgtaaca
    19681 gagcacgcgc ccgtaggcgt ccgtgtcgag ccgcgccgtg aagagatcgc catcgacctg
    19741 tgccttataa cgatagaccc gctccgcctc acgcgtgtac atctgctcca gcggctcgag
    19801 ccgcagctcg ggaaaacgga gccacgcgct ccgcaccggc gcggtctcgc cgaccacgag
    19861 cgaaaggcgc cggatgggca gcgtgttggt ggccggcgtg aacccgaggt cgacgtcgag
    19921 cgcgccggtg agcccgggga gcgcctggcc gtcgcgcgtc cagtggccct cgccatcggc
    19981 ggccagagcg aaccggacag ggcggccccc catctcgccc tcgatcaagg cggagcgggt
    20041 gcgccactcg ggatcgcatt cgatgcagta gcggagccgg gcgggaacgc cttcctcttc
    20101 gacgtccagt tgacccgtga ggcaccaacc atcgggcgac cgctcgacgc gagcgtcctc
    20161 gcggccaggc acatccaggc gccgccagcg catgtgcgat tcggatggaa acgggctcag
    20221 cgtcatcttg gcctctcggc gtgcggcgtc aagaatatat cccgcgccac cctttccggg
    20281 gggcgggcct cagcgcaggg tgactgtcgg gttgcggacg aaacgggcgc ggtagggcag
    20341 gcggtcgttc agggcgacga gaagacggaa gaggcgcggg tgctgggtcc agagcagctt
    20401 ccagcgcagg cgcaggccgc ggcggcgcag gcggccgaag tggttgacgg cgtccagggc
    20461 gcggcgcgcc ccttcccagt cgcgcgccga gcaggcccgg acgtagtcgt ccagcgaggt
    20521 gaagaagaag cgcggcgcga tcgcgtccgg gacgtagtag cggaacatgc gcgtgtcgtc
    20581 gggcagcatc gccgcgagct tcgccgcgta cctcatgcgg ctgtcgatga agcggcgcga
    20641 cgccgaggag ccggtcgaat ggatccggaa acgcagcagc ggacggtcca cgaagacgtg
    20701 ctcccagccg agccggaaaa agcgcacgaa caggtcgtcg tcctcatagc cggagagctc
    20761 ctcgtcgaag ccacccgcct gctcgaacgc ggcacggcgg acgatgctcg ccgaagggag
    20821 caccatgaga tcaccggcca cgcattcgaa aatcgtccgc ttgggatgcc tcatccccgc
    20881 cgcgcgcagg aacgcacggg tcaccaggtg gccgtcgaag tccatctcgt cgaagtcgca
    20941 gtagacccaa cccacggccg ggtcgtcctc gagcggcggg atcaattgct cgagatgctg
    21001 gggatgccac tggtcgtcct ggtcgaggaa ggccaggtac tccccctccg cctggcgcgc
    21061 cgcgaggttg cgcgccgccg actgcccggc gttctcctgc cgcagcacgc ggatcgggaa
    21121 gggagctggc ggcaagccct ccagctcggc gagcgagccg tcggtcgagc cgtcgtccac
    21181 cacgatcagc tcgcagggag gcagagtctg cgcggccacg ctgcggacgg cgtcgcgcag
    21241 gtagcggcga ccgttgaaga ccggcaggat ggcggagacg cccggcatca gggggagcct
    21301 ccggggctga ccggacggag gcccgcgacc ggatactgcc gcgagcgcgt ctcgctctcc
    21361 cccgtcgcca gcggccaggc cacggcgccg ggttggacct cggccacctt gcgcgccagg
    21421 atctcggacg tcgcgtaggc cgggacggtg ggcatgtcga ggaagccctc gcgccattcc
    21481 gggccgggga tgatcgccgc cggcccggag tcgcgggcgt ccaggatctc gaagccgttc
    21541 tccggcccga acagcacgcg cagaccctcg gccgagatgc gccagaagtc gttgggctgc
    21601 gcgtgggccg gccaggctcc cggtgcctgg tgatagacca gcccgccggg cttgagcacg
    21661 cggttgatct cggccgccag cagccagggc gcctggaggt gttcgaggac cgaggcggag
    21721 agcaccgcgt ccaccgagcc cgcgcggagg aacgtgctca agcggtgagc gtcgcccacc
    21781 acgtcgactt tgaggccggg gtggatgtcg agcccgatca cgcgcccctg cagcagatgg
    21841 cggcgcggga tcggctccgt gccgctcggc gtgcgtgccc cgatctggag cagccggctg
    21901 tcacgcccgc cgcgggcgtt gacgagctcg gccaggcgcc gcagcagcgg cgactccccg
    21961 aggtcgaagt cgtcggaccg cggccagggc gggagcggac cttcggggag acggagtggg
    22021 aagctcgcgg cgccgccgtc ggtctccagg gtcaggacca caggatggcc cggcgggcag
    22081 gcgaggtaca cggcgaagcc gccatggcgg acgtgctcgt gctcgggata gtgattgagc
    22141 aggtcggggc ggtcggtaaa ggcctccacc cgcgcggacc ggccggccga ttcgacccgc
    22201 agggcgcgca cccggtgctc gtagg
//
LOCUS       DASPJI010000026         9820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_460236, whole genome shotgun sequence.
ACCESSION   DASPJI010000026 DASPJI010000000
VERSION     DASPJI010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9820)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460236"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="VEL74_00745"
     CDS             <1..1360
                     /locus_tag="VEL74_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61080.1"
                     /translation="AFSGFVFLGGPGLTYRIGLTSLFINLSIGFTAALLGWTVAKRLR
                     LLTEVREVYTIPDAVLVRYGSRAASGAAAVAILVGTVGYLGTQLLALGRLLEPVLGLR
                     ELAGEQSLLVGVGIGLAVLLFYSVAGGMVAGVYTDLFQGTLMILAACAVFYYAMSAGG
                     GLETMAASIAGSERFGRQFLEPFGRTPVLTAVGFWFVFGIGVLGQPHMLHKFFMLRDP
                     RHLRWLPVTLGGSQILCLLIWFGIGLAVPALVAQGKLPPLEQPDDAAPVFLLLFAPEV
                     VTGLAVAGILAAIMSTADSFMNIGAAVLVRDLPRALGRRVGDEMLWGRVATLGVSLAA
                     AGLAFAYGDLIALLGTFSFGTLGAALAPALAVGLNWKRVTAGAATASIVTGTVLNLTL
                     EFLGRQTFFPSLPKPPLPPGVLPTAVSLAASFTILLAVSWWTGRRSTPSLPEDVATVM
                     EI"
     gene            complement(1364..2905)
                     /locus_tag="VEL74_00750"
     CDS             complement(1364..2905)
                     /locus_tag="VEL74_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HYG61081.1"
                     /translation="MTVDTESSITQILDSLGLEAENPGAFDGTWLRGSGERVESINPA
                     TGEPIAAVRMGTEQDYEKVSAATVEAFREWQTWPAPRRGEVVRQLGEELRRHKEELGV
                     LVSLETGKVLSEGRGEVQEMIDMADLAVGMSRQLYGLSMHSERPKHRMYEQWHPLGPV
                     GIITAFNFPVAVWAWNALVAAVCGDSMIWKPSHLTPLTAVATTRIAERVLRANSAPPI
                     FNLILGGRTGVGERLVADRRFPLISATGSGRMGREVGKVVADRLGRTLLELGGNNGIL
                     VMDDADLDLALRAVLFAAVGTAGQRCTTTRRLFLQRGIAAEMKRRLVQAYGSISIGDP
                     LETGTLMGPLIDQNAVDNMMRALEQVKAEGGKVLYGGKRIDQPGFFVEPTLVEAKGGM
                     PITCEETFAPILYVFEFDELDEAIRLHNDVPQGLSSAIFTLNMRSAERFLASTGSDCG
                     IANVNIGTSGAEIGGAFGGEKETGGGREAGSDSWKAYMRRQTCTINWSTELPLAQGVE
                     FKVGA"
     gene            2996..4972
                     /locus_tag="VEL74_00755"
     CDS             2996..4972
                     /locus_tag="VEL74_00755"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HYG61082.1"
                     /translation="MRSARFVPPLLLALFGGWCGTFGWGATFLGWATAIAAVLGTLLA
                     VGAPWRDSLSLGTAGRWLPPALWVTMAASAWASPVPRAGLTGVVLLPAFLWLPAAVEH
                     CWSAEDDRRQGLRAVAAVVTGMSLWALVDLALLGASRETWRAIMPLGHHNLLATWMVI
                     LLPLAGLSARERGLWRALAFTACLLGVAVVLATRSLAGSLALTAEVCLILGGMLHRLS
                     GSTGRRLRLAGALLALLMLVVALVQVPRVKEILKGNDLSVRARTVYYAAAWEGFLARP
                     VLGWGPGSAAWTAASFLDPAPGVRPWGEAVGELHSLPLQVAYESGATGLLLALAVAGL
                     FGWRRLARRGEARDPMLVAAGLLGLLGAAVASLGSGALAVTALPLAAAVAAGAALAGG
                     EVNARGSTLPSRIYAVAAGMVLLPAALALSHYDRALAHGTAGRQGEARWELTQAVRLD
                     PSFPLYRMRLALLQEGAAAARLALQAAQDSHAVPVLWLIAGLDGYRAGEPSAREALSR
                     ACNLDPLGGFAPFYLMLSAPESPQAPAYGAHALLAEPRLAAAVFLERQEELFQRTLHE
                     AQAWPGLSPSWREALPVNSPAAAERTGERAWLGLGLDTTPSLALSLHTFRRRPWPTWW
                     EVVEVHRLPAERLQLLPPAFSDIGQAAPRSCSPL"
     gene            complement(5320..6663)
                     /gene="lat"
                     /locus_tag="VEL74_00760"
     CDS             complement(5320..6663)
                     /gene="lat"
                     /locus_tag="VEL74_00760"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HYG61083.1"
                     /translation="MSTTTVETSPRILPEEIHPRLGRHMLVDGYDLVLDLGRSRGAFL
                     YDSLRGGEILDLFTCVSSCALGYNHPKLDTPEFRERILPAAINKPSNPDVYTEQMAQF
                     VEAFARTLPAPLASHLFFIDGGALAVENALKTAFDWKVRKNLAAGRGEKGSQVIHFRQ
                     AFHGRSGYTLSLTNPADIRKTQYFPKFPWPRITNPKLSFPTTPEVLEQVIAAERQAIA
                     EIEQALVENRDDIAALIIEPIQGEGGDNHFRGEFLRELRRLADEHDFLLIFDEIQTGF
                     GMTGRWWSCEHFDVLPDIIAFGKKTQVCGIAVSNRIDDVESVFKVPSRINSTWGGNLV
                     DMVRCTRFIEIIEEDGLLANAERVGRRLLDGLRRLEEVFPGKVTNSRGRGLFVAFDLP
                     DQALVNKVLDTLLAQGTMALPSGVHSIRFRPALVLQPEEADEALRRVEKALLSVF"
     gene            complement(6775..7971)
                     /locus_tag="VEL74_00765"
     CDS             complement(6775..7971)
                     /locus_tag="VEL74_00765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HYG61084.1"
                     /translation="MSDPLLRTDGPPEGLPDVEECHCLDHVQLERFLERRRANPTFPL
                     EISLAESLTEALRRANSFVPSAAGSILLDNPQEKQEDRRRNTLTFIAAFGEKADGLVG
                     KTIPADHGIAGRVYLTGEGYVTPNAPGDRFFYRGMDEQTRYQTHSLIAIPIRIEQEVC
                     GVLELINRRHSSGYSEQDRNLLEIFAGYISISIQNVLDGRQAQEIAKRDNLTGLFNDR
                     YLHIATSDSIRRCKTEGLDLAVLFLDLDFFKRVNDTHGHLAGSQVLREVGHLLRQKTT
                     AVGGLAARYGGDEFVVLVPGFDMGDAIDLAEEIRASVVSTTFSAQPGEIQPEPLYLKG
                     LTCSIGVATMRLHLDNDVSVDVCKSSLLRLADAAMYVAKETGRNRTATAGQPVPRRTS
                     PVPSAR"
     gene            complement(8111..8836)
                     /locus_tag="VEL74_00770"
     CDS             complement(8111..8836)
                     /locus_tag="VEL74_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG61085.1"
                     /translation="MSQWPASLAAAALLALLLLGAVPLPVHGAAADTETALVEGNRRF
                     RDGDYEGAFETYAAGYGSGSSNPVLAYNLGVAAHRLERLPEAILWYRRAQLSLGADPW
                     LQDNLELARSALGTPERPRPPGARWVERRSVFLLAGTVLAWSLLLLALRSPSSRQRRR
                     WPVALAAALSLLFFAAGTLLGRLGPQAAVLLADCPGVADGGLPSGTEVWVRGDDSGWR
                     VLGEPEAVCPVEAVALVEDPAVH"
     gene            complement(8833..>9820)
                     /locus_tag="VEL74_00775"
     CDS             complement(8833..>9820)
                     /locus_tag="VEL74_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HYG61086.1"
                     /translation="RFFGPPLSRSEQLLLRTDPKAVEVRPLPPAPPGFGGAVGDLSLA
                     AQLEPREVRLGEAATLTLTLAGEGNLQAVQPPPVAPAPGVEVLPPQQHGEEKMQGTEI
                     HGSRTWSFVVIPGRTGRHELQVPPVSFFDPETGQYRTASVAPVELTTLPRAPQPGSGE
                     PSLHGIRSASFAGSAGGAAGGLVTRWRDLLPWLFSLPWALALLVVLARRRTMPAATAG
                     EEAADPSAEARLQQRLGEARTEARPRQAAAQIEEAWREFLAARWDIPCATHSARWSAM
                     LEERGADPEAARELVQLVDDLHYLRYAPQLSSTDTLRGEALDRCRRLVRRLR"
BASE COUNT         1357 a         3300 c         3548 g         1615 t
ORIGIN      
        1 ggcgttcagc ggcttcgtct tcctcggcgg cccggggctg acctaccgga tcggcctcac
       61 ctccctcttc atcaacctgt ccatcggctt caccgcggcc ctcctgggct ggaccgtagc
      121 caagcggctg cgcctgctca ccgaggtgcg ggaggtttac accatccctg acgccgtcct
      181 cgtccgctat ggcagccggg cggcttcggg cgcggcggcg gtggcgatcc tggtggggac
      241 cgtgggctat ctcgggacgc agctcctggc actggggagg ctgctggagc cggtgctggg
      301 gctgcgcgag ctggcggggg agcagagcct cctggtgggg gtggggatcg gccttgctgt
      361 ccttctcttc tacagcgtgg ccggaggcat ggtggccggc gtctacaccg acctgttcca
      421 ggggacgctg atgatcctcg ccgcctgcgc cgtcttttac tacgcgatga gcgcgggagg
      481 ggggctcgaa accatggcgg cgagcatcgc gggctccgag cgcttcggac ggcagttcct
      541 ggagcccttc ggccgcacgc cggtcctcac tgcggtcggc ttctggttcg tcttcggcat
      601 cggtgtcctc ggccagccgc acatgctcca taagttcttc atgctgcgtg acccgcggca
      661 cctccgctgg ctgccggtca ccctcggcgg ctcccagatc ctctgcctgc tgatctggtt
      721 cggcatcggc ctggcggttc cggcgctggt cgcccagggg aagctgccgc cgctggagca
      781 gccggacgat gccgcgccgg tcttcctcct ccttttcgcg ccggaggtgg tgacagggct
      841 tgcggtggct ggcatcctcg cggccatcat gtccacggcc gactccttca tgaacatcgg
      901 cgcggccgtc ctggtacgcg acctgccgcg ggctctcgga cgccgggtcg gtgacgagat
      961 gctgtggggc cgggtggcga cgctgggcgt gagcctcgcc gcggccgggt tggccttcgc
     1021 ctacggtgac ctcatcgccc tgctggggac cttctctttc ggcacgctcg gagccgcgct
     1081 tgcccccgca ctcgccgtgg ggctcaactg gaagcgggtc accgccggcg cggccacggc
     1141 ctccattgtc actgggacgg tgctcaatct gacgctggag tttctggggc gccagacctt
     1201 tttcccctcc ctccccaagc cgccgctgcc tccgggggtc ttgccgacgg cggtgtcgct
     1261 ggcggcctcg ttcacgatcc tgctggcggt gagctggtgg acggggcgcc ggagcacccc
     1321 gtccctgccg gaagatgtgg cgacggtgat ggagatctga gaatcaggcg ccgaccttga
     1381 actcgacgcc ctgcgccaac gggagctcgg tgctccaatt gatggtgcag gtctggcggc
     1441 gcatgtaggc cttccaggag tcggagccgg cctcacggcc gccgccggtc tccttctcgc
     1501 cgccgaacgc gccaccgatc tcggcgcccg aggtaccgat gttgacgttg gcgatgccgc
     1561 agtcgctgcc agtggacgcc aggaagcgct cggcgctccg catgttcagg gtgaagatcg
     1621 ccgacgacag tccctgcggc acgtcgttgt gcagacggat ggcctcgtcc aattcgtcga
     1681 actcgaagac gtagaggatc ggtgcgaagg tctcctcgca ggtgatcggc atgccgccct
     1741 tggcctcgac cagggtgggc tccacgaaga agcccggctg atcgatccgc ttgccgccgt
     1801 acaggacctt gcccccctcg gccttcacct gctccagggc ccgcatcatg ttgtccaccg
     1861 cgttctgatc gatgaggggg cccatgagcg tcccggtctc caggggatcg ccgatagaga
     1921 tggagccgta ggcctgcacc aagcgccgtt tcatctcagc ggcgatgccg cgctggagga
     1981 agaggcgccg cgtcgtggtg cagcgctggc cggcggtgcc gacggcggcg aagagcacgg
     2041 cgcgcagggc gaggtccagg tcggcgtcgt ccatcaccag gatgccattg ttgcccccca
     2101 gctcgaggag cgtccggccg aggcggtcgg cgaccacctt gcccacctcg cggcccatgc
     2161 ggcccgagcc ggtggcggag atcagcggga accggcggtc ggccaccagc cgctcgccga
     2221 cgccggtgcg gccgccgagg atcaggttga agatcggcgg cgcggagttg gcgcgcagca
     2281 cccgctcggc gatccgggtg gtcgcgactg cggtcagggg ggtcaggtgg gagggcttcc
     2341 agatcatgga gtcgccgcag accgccgcta ccagggcatt ccaggcccag accgccacgg
     2401 ggaagttgaa agccgtgatg atgcccaccg gccccagcgg gtgccactgc tcgtacatgc
     2461 ggtgcttcgg gcgctccgag tgcatggaga ggccgtagag ctggcgcgac atgccgaccg
     2521 ccaggtcggc catgtcgatc atctcctgca cctctccgcg gccttccgag aggaccttgc
     2581 cggtctcgag gctcaccagg acgcccagct cctccttgtg gcgccgcagc tcctcgccga
     2641 gctggcggac gacctcgccg cggcgcgggg ccggccaggt ctgccactcc cggaaggcct
     2701 ccacggtggc ggcggacacc ttctcatagt cctgctcggt gcccatgcgg accgccgcga
     2761 tgggctcgcc ggtggcggga ttgatggact cgaccctctc ccccgatccg cggagccagg
     2821 tgccgtcgaa ggcgccgggg ttctccgcct ccaggccgag ggaatcgagg atctgggtga
     2881 tgctgctctc tgtatcgacg gtcatcggat gtctcctgga acgtacgggt gcgggtactg
     2941 cgggaggacc caaggatatc gcaatcggcc cccttacaat ccgtggatgc gtatagtgcg
     3001 ctcggcccgg ttcgtgccac ccctcctcct cgccctcttc ggcggctggt gcggcacctt
     3061 cggctggggg gcgaccttcc tgggctgggc aaccgccatc gccgccgtcc tgggcacgct
     3121 gctcgccgtg ggggctccct ggcgcgattc cctgagcctg gggaccgccg gccgctggct
     3181 gccgccggcg ctctgggtga cgatggcggc gagcgcctgg gcctcgcccg tgccccgggc
     3241 cgggctgacc ggggtcgtat tgctccccgc cttcctctgg ctgccggccg cggtggagca
     3301 ctgctggagc gccgaggacg accggcggca gggactgcgg gcggtggctg ccgtggtgac
     3361 gggcatgtcc ctgtgggccc tggtggacct tgccctcctg ggagcctccc gggagacgtg
     3421 gcgggcgatc atgccgctgg ggcaccacaa tctgctggcg acctggatgg taattctact
     3481 ccctctggcg ggcctctccg cccgcgagag gggactctgg cgcgctctgg ctttcacggc
     3541 ttgcctgctg ggagtggcgg tggtcctcgc gacccgctcc ctggccggct ccctagcgct
     3601 gacggccgag gtctgcctga ttctgggagg aatgctccac cgtctctccg gttccacggg
     3661 ccggcgcctg cggctcgcag gggcgctctt ggccctcctg atgctcgtgg tggccctggt
     3721 ccaggtccca cgggtgaagg agatcctcaa ggggaacgat ctgtcggtgc gggcgcggac
     3781 cgtctactac gcggcggcct gggaggggtt cctcgcccgg cccgtcctcg gctgggggcc
     3841 cggttccgcc gcctggaccg ccgcctcctt cctcgatccg gctccgggag tgaggccctg
     3901 gggagaggcc gtgggggagc ttcactccct tcccttacag gtcgcctatg aatccggggc
     3961 cacggggctc ctgctggcgc tggcggtggc gggcctcttc ggctggcgcc ggctggctcg
     4021 aagaggggag gcccgcgatc cgatgctcgt cgccgccggc ctgctggggc tcctcggagc
     4081 cgccgtggca tccctgggta gcggcgccct ggccgtgacg gcgctcccct tggccgccgc
     4141 cgtcgcggcc ggagctgctt tggcgggagg agaggtgaat gccaggggga gcacgctccc
     4201 ctcccgtatc tacgcggtgg cggccgggat ggtcctcctt cctgcggccc tggccctgag
     4261 ccactacgac cgggcgctcg ctcatggcac cgccggtcgt cagggggagg ctcggtggga
     4321 gctgacccag gcggtgcggc tcgatccctc cttccctctc taccgcatgc gccttgcgct
     4381 gctccaggaa ggggccgccg ccgcccggct cgccctccag gccgcccaag acagccacgc
     4441 cgtccccgtt ctctggctga tcgccggact ggacggatac cgcgcgggag agccctcggc
     4501 ccgggaggcg ctgagccggg cgtgtaatct cgatccgctg ggaggcttcg cccccttcta
     4561 cctcatgctc tccgctcccg agtccccgca ggctccggcg tacggagccc acgcgctgct
     4621 ggccgagccc cggctggcgg ccgcggtgtt cttggagagg caggaggaac tctttcaacg
     4681 caccctccat gaggcccaag cctggcccgg cctgtcgccc tcctggcgcg aggcgctgcc
     4741 ggtcaattct cccgcggcgg ccgagcgcac aggggagcgg gcctggctag gccttggcct
     4801 cgacacgact ccatccctgg cactctccct ccataccttc aggcgccgtc cctggcccac
     4861 ctggtgggag gtcgtggaag tccaccgcct cccggccgag cgcctgcagc tcctgccccc
     4921 tgcattcagt gacatcgggc aggcagcccc ccgctcctgc tcacctcttt gaccgtcgtc
     4981 ggaccacccg atgcacaccg cctgagcacc ctgccgatct ccacagccgc tgtggaaaac
     5041 cctgcgggaa aaccccctcc ggaggcagga aacacgctgt gggatagtca tttggtcctc
     5101 tttgaacaga tttctgtgca atcgagataa gtagtttgaa atcagtgact tgaaaaatct
     5161 ttctgcgcct tgggcgaggc tctttgccaa aaggaaacgc cctgttgcag gttttccaca
     5221 gtttgcgctt cttcctgtgg agagcgcaag tggaacggct cccgcgggag gcttcgtgcc
     5281 tccccggaag ggtgtctcga gagagtgggc tggagcggtt tagaagaccg agagcagagc
     5341 cttttcgacc cgccggagag cctcgtccgc ctcctcgggc tggagcacga gggcgggacg
     5401 gaagcggatc gagtggacgc cggaaggcag ggccatggtg ccttgggcga ggagcgtgtc
     5461 gagaaccttg ttgacgagag cctggtccgg caggtcgaag gcgacgaaca ggccgcgtcc
     5521 gcggctattg gtgaccttgc cggggaagac ctcttccagg cgccgcagac cgtcgaggag
     5581 acgccggccg acccgctcgg cattggcgag gaggccgtcc tcctcgatga tttcgataaa
     5641 gcgggtgcag cgcaccatgt ccaccaggtt acccccccag gtggagttga tgcggctggg
     5701 gaccttgaac accgactcga catcgtcgat gcggttcgag acggcgatgc cgcagacctg
     5761 ggtcttcttg ccgaaggcaa tgatatcggg cagcacgtcg aaatgctcgc agctccacca
     5821 acggccggtc atgccgaagc cggtctggat ctcgtcgaag atcagcagga agtcgtgctc
     5881 gtcggccaga cgccgcaact cgcgcaggaa ctcgccccgg aagtggttgt cgcccccctc
     5941 gccctggatg ggctcgatga tcagggcggc gatgtcgtcc cggttttcca cgagtgcctg
     6001 ctcgatctca gcgatcgcct gccgctcggc cgcgatcacc tgttcgagca cctccggagt
     6061 cgtggggaag gagagcttgg gattggtgat gcgcggccac gggaacttgg ggaagtactg
     6121 ggtcttccgg atgtcagcgg ggttggtcag cgacaaggtg tagccgctgc gtccgtggaa
     6181 cgcctgccgg aagtggatga cctggctccc cttctccccc cggccggcgg cgaggttctt
     6241 cctcaccttc cagtcgaagg cggtcttgag ggcgttctcg accgccaggg ctccgccgtc
     6301 gatgaagaac agatggctcg ccaggggcgc cggcagcgtg cgggcgaacg cctcgacgaa
     6361 ctgcgccatc tgctcggtgt agacgtccgg attggacggc ttgttgatcg ccgcggggag
     6421 gatgcgctcc cggaactcgg gggtatcgag cttcggatgg ttgtagccca gggcacagct
     6481 cgagacgcag gtgaagagat cgaggatctc gccgccgcgc aggctgtcat agagaaaggc
     6541 gccgcggctc cgcccgagat cgagcaccag gtcgtagccg tccaccagca tgtgtcgtcc
     6601 gagacgtggg tggatctctt cgggcaggat gcgggggctg gtctcgaccg tggtggtgct
     6661 cattgacgac tctctcctga tggaacctgc gggacggatg gcgtcctgac tgggcgaggt
     6721 tgcaacgtct cgcgggagcg actcagggat cgtagcaccc ccctcgcgag ggggtcaacg
     6781 agccgagggg acgggagagg tgcgccgggg gaccggctgg ccggcggtgg ccgtacggtt
     6841 gcgcccggtc tccttggcca cgtacatggc ggcatccgcc agacgcagca gcgagctctt
     6901 gcagacgtcc accgagacgt cgttgtcgag gtggagcctc atcgtcgcca cgccgatgga
     6961 gcaggtcagc cccttgaggt acagcggctc cggctggatc tcccccggct gggcgctgaa
     7021 ggtggtgctc accacggacg cccggatctc ctcggcgagg tcgatcgcgt cccccatgtc
     7081 gaagcctggg accagcacca cgaactcgtc gccgccgtag cgggcggcca ggccgccgac
     7141 ggccgtcgtc ttctgacgca gcaggtgccc gacctcgcgc agcacctggc tgcccgccag
     7201 gtggccgtgg gtgtcattga cccgcttgaa gaagtcgagg tcgaggaaca gtacggcaag
     7261 gtccagcccc tcggtcttgc agcggcggat ggagtcggag gtggcgatgt gcaggtagcg
     7321 gtcgttgaac aggccagtca ggttgtcgcg cttggcgatc tcctgggcct gccggccgtc
     7381 gagcacgttc tggatggaga tcgagatgta gccggcgaag atctccagca ggttgcggtc
     7441 ctgctcgctg tagccggagc tgtggcgccg gttgatgagc tccaggacgc cgcagacctc
     7501 ctgctcgatg cggatcggga tggcgatcag cgagtgggtc tggtagcgtg tctgctcgtc
     7561 catcccgcgg tagaagaagc ggtcgccggg ggcgttgggg gtgacgtacc cctcgccggt
     7621 caggtagacc cggcccgcga tcccgtggtc tgcggggatt gtcttgccca ccaagccgtc
     7681 cgccttctca ccgaaggcgg cgatgaaggt cagggtattg cgccgcctgt cctcctgctt
     7741 ctcctgcgga ttgtccagga ggatcgagcc ggccgccgac gggacgaagc tgttggcccg
     7801 gcgcaaggct tcggtcaggc tctcggcgag gctgatctcc agcgggaagg tcgggttggc
     7861 ccggcgccgc tccaggaaac gctcgagctg cacgtggtcc aggcagtggc actcctccac
     7921 gtcggggagc ccctcgggag ggccgtcggt ccgcaggagg ggatcgctca aggcgttcct
     7981 ccagagactg tgcaaatgct cgtcgacgtg cgcggttcca tgcaggacga tcccagaagc
     8041 tccgtattga cggtcggcaa agggaaagat accagaagtt ccaccaaggc gaaaagcact
     8101 tcagcgattt tcagtggacc gcggggtctt ccacaagggc gaccgcctcc accgggcaga
     8161 ccgcctcagg ctctccgagc acccgccagc cactgtcgtc gccccggacc cacacctcgg
     8221 tgccggaggg gaggccgccg tccgcaactc cggggcagtc agcaagaagg acggccgcct
     8281 gggggccgag cctgcccagg agcgtgccgg cggcgaagaa gagcagcgac aaggcggccg
     8341 ccagggcgac cggccaccgg cggcgttgtc gggacgaggg ggagcggagg gcgaggagga
     8401 ggagggacca agcgaggaca gtgccggcga ggagaaagac ggagcgtctc tcgacccagc
     8461 gggcgccggg ggggcgcggc ctctccggtg tgccgagggc gctgcgggcg agctccaggt
     8521 tgtcctgcag ccaggggtca gcgccgagtg agagctgggc acgccggtac cagaggatcg
     8581 cctcgggcag ccgctccagc cggtgggcgg cgacgcccag gttgtaggcg aggaccgggt
     8641 tgctggagcc ggagccgtag ccggcggcat aggtctcgaa cgccccttcg tagtcgccgt
     8701 cgcggaaccg ccggttaccc tcgacgaggg cggtctcagt gtcggcggcc gcgccgtgga
     8761 ccggcagggg gacggcgccc aggagcagga gggcgagcag ggccgccgcg gcgagcgagg
     8821 cgggccactg gctcatcgca ggcggcggac gaggcggcgg cagcggtcca gggcctcgcc
     8881 gcgcagggta tcggtggagc tgagctgcgg cgcgtagcgc aggtagtgga ggtcgtcgac
     8941 gagctggacc agctcccggg cggcctcggg gtcggccccg cgctcctcca gcatcgcgct
     9001 ccagcgcgcc gagtgggtgg cgcacgggat gtcccagcgg gcggcgagga actccctcca
     9061 tgcctcctcg atctgggcgg cggcctgccg cgggcgggcc tcggtccggg cctcgccgag
     9121 ccgctgctgc aagcgcgctt cggcggaggg atcggcggcc tcctcgccgg ctgtcgcggc
     9181 cggcatcgtc cgcctccggg ccaggaccac cagcagggcg agagcccagg ggaggctgaa
     9241 gagccagggg aggagatccc gccaccgggt gaccaggcct ccggccgcgc ccccggcgct
     9301 gccggcaaac gacgcgctac ggatgccgtg gagcgatggc tcgccggagc ccggctgcgg
     9361 cgcccgcggc agggttgtga gctccaccgg cgccaccgaa gcggtgcggt actggccggt
     9421 ctccggatca aagaaggaga ccggcggcac ctgcaactcg tgcctgccgg tgcggccggg
     9481 gattacgacg aagctccagg tgcggctgcc gtggatctcc gtcccctgca tcttctcctc
     9541 cccgtgctgc tgcggcggga gcacctcgac cccgggggcg ggcgcgaccg gcggcggctg
     9601 cacggcctgg aggttgccct cgccggccag ggtcaacgtg agggtcgcgg cctcgcccag
     9661 gcggacctcc cgcggctcga gctgggccgc cagggaaagg tcgccgacgg cgccgccgaa
     9721 gccgggcggc gcggggggca gcggccggac ctccaccgct ttcgggtcgg tgcggagcag
     9781 gagctgctcc gaacgggaga gcggtgggcc gaagaaccgc
//
LOCUS       DASPJI010000027         8314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_58392, whole genome shotgun sequence.
ACCESSION   DASPJI010000027 DASPJI010000000
VERSION     DASPJI010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8314)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58392"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="VEL74_00780"
     CDS             complement(<1..1306)
                     /locus_tag="VEL74_00780"
                     /inference="COORDINATES: protein motif:HMM:NF026288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG61087.1"
                     /translation="MTHDRVAVALLILTLGTLAAVGWGVSRPGPKRTLRSRPSAAAEP
                     RPASLAAPAPPVPLALKVLTNGGRQRHHPPPGETRRYTLPLSKDQYLHLVVEQFGVDI
                     AATIRDPSGQRLLRVDSPTDDKGPEDLFLATGSAGPYLLEVASPEGAGGGRYEIRIEA
                     LRVATDEDRDRAAAAAAFSMARLIQREETGSPEAAAAAYRDAARLWRKVGDEEREAWA
                     LYRLGKMYSDDSGRCCEATAALERAVQLFGEVSRPKQQALSLYYLGEARACRGDLDSA
                     AGTYEQALTSWEQQGNRFEQATTLNSLAIVRRYQGRTHTAIDLFSRAVEIWHELREWG
                     DLATTHANLGVLYVSLGESRLALEQYRRALAFLERQPDPLQKAVILSKQGDALLGIGR
                     LEEALERYEEALALRRRWREIRGEGITLNGIGRVNLAMNRPRE"
     gene            complement(1406..2194)
                     /locus_tag="VEL74_00785"
     CDS             complement(1406..2194)
                     /locus_tag="VEL74_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYG61088.1"
                     /translation="MSSAEPAERLRAHFKRERRAIDQHPTPERIVAYHECRLVPDEAE
                     EIRAHLVACPDCTAELLDLAALLEGEDDATSEISPAELDAAWQRQRARRLPDRPAAPP
                     ERPWHRPAPPRRAWATAAVLGLAAALLAVVALDQRRTIALLQQPQANPPLVNLEPVDS
                     VRQGLREAPELRLPAQGERIWMILNPSAELAASTYGIEVVAPDGAVVIRLEDIPSSEA
                     GNFRLEIPRGALEAGSYRVLLEESTSGERRIVEEFALRVRPAAP"
     gene            complement(2288..2902)
                     /locus_tag="VEL74_00790"
     CDS             complement(2288..2902)
                     /locus_tag="VEL74_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYG61089.1"
                     /translation="MAPGREDDLDLQRAVTELQAGIRVELNSRRIFDSFYPWIHRFFR
                     HRGYAPQDCEDLAQETLFQVFDRIDSYRHEGPFKSWVFAVAANLHRRKYRWRHREKRN
                     ATEVSITPATEEDPVAPEPVAPGDSPAREAYERERREALAQALKRLPPQMRQVLTLRI
                     DQDLKYREIAEVLHISIETVKAHLFQARQRLRAELGEEYGEWVE"
     gene            3170..4402
                     /locus_tag="VEL74_00795"
     CDS             3170..4402
                     /locus_tag="VEL74_00795"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HYG61090.1"
                     /translation="MADLAAASGFGLFQHGGRAIGQVGALTARAPDPSALTYNPAAIT
                     QLEGLQAMAGLDFNNAEDAYESPTGRFAADHIIQFPPLAYLTWKPKDSSFALGIGLDA
                     PFWYVEDWEPALFPGRFISRRFMLRVYELHPVAAWEMGGGWSIGGGVRYLFGSMEQDD
                     SIISQFFIPGTGNVPVELERNADADVDDLTYDLALHYTQPAWGWGVVYRGAANLKGNG
                     DLDFQPRNVPNIPGLQTQIDQRFGSGSSRQSFEIPRELRGGIWVAPYPELRLELDASW
                     QSWSSLEATSITYSNPIGTLSEEIPRDWDDTISLRLGVEGEITEAFMLFGGVAYEPSP
                     ATGENAAPDFPRSDAMVYAAGFSYGFPQISFDVGYSFHEHNSRSVEGQEPLTNPTRSG
                     RYTSSDQVWGFGVRWRFQ"
     gene            complement(4473..6491)
                     /locus_tag="VEL74_00800"
     CDS             complement(4473..6491)
                     /locus_tag="VEL74_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HYG61091.1"
                     /translation="MKPPEIIRLTPGKRVLFLTKDPDLIRRQLRGELDLRMEDLRIED
                     LLDDINTDAMTPAWVCFSHRPEDIARDAYAGLIVDGKRLFERDALINGNFEVIVSGYR
                     KGVGSSRETAVQAEKWSGIRLAIAASFAPIHARNNISQGVLMGDHDQLRRLQTGEGIP
                     LEEFCEGWDPISRLVIQHGGLFPFSKAVAAGEAGVPRPDTGPRPMTMAEKILADHMLG
                     VDDAHRFVKPGDAVVVQVDGGYTHEFTTAQVHYFLEQEYGPDYEARNPDKFAVFEDHL
                     IYADEVPKMRAFSDKIETLRQLQREFQRHTGVRDFSARDGISPGICHEVAREHLIDPG
                     DFIQATDSHTCMGGVNNALAWGVGATEYADLVHSGFTQVEVPESIRFELTGKLQPGVT
                     AKDLMLYILLEYARPQLTLNRVMEFTGPGVATLSMDERATLTNMATECAARTAIVIAD
                     DETFRWLAEWRPGVDVEELRARAVAPDPDAHYDGGVHSIDLSALRPMVAHPGNPDQGI
                     PSDPTNGAWIDEIGDVPIDIAYGGSCTAGKIDDLIFYHQVVKEAVDAGLQVADGVKFL
                     IQCGSVAVEDFVRRNGLADTFERAGVTLIHPGCGACIGCGPGVSDEPDQVSVSAINRN
                     YKGRSGPGQLWLASPLTVAASAFTGRITAYTPGMFREIAAHQPELAPV"
     gene            complement(6636..7430)
                     /locus_tag="VEL74_00805"
     CDS             complement(6636..7430)
                     /locus_tag="VEL74_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61092.1"
                     /translation="MLFPSERSPEEARDMIRRLLARVPERASEGAGEEPVPAAAAQVE
                     PPAGAYDRAFTAAFERVDRHALDLSREHAAAAALWAELLGHDAGRRRMRVRNDRRYHS
                     WAFCDRLLEESRKAGRSDPRRALEIAELALEAAHHLDTEHYGQERLADLLGMSWCAVG
                     EARRLQGDREGARGALDCAREALARGTGDPLARAALAGHDASLAMDLHEYAEAAGHLD
                     RAIHLHRRYGDPDFAGDRLVQEALRRAGVRRAAPASRASRHGRRSG"
     gene            complement(7670..>8314)
                     /locus_tag="VEL74_00810"
     CDS             complement(7670..>8314)
                     /locus_tag="VEL74_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYG61093.1"
                     /translation="RIEPGEIEASLMAHPAVAGALVMAREDVAGDRRLVAYVVLLPQE
                     SPALPAVDTAALRAWLGERLPHYMIPARFLFLDELPLNPNGKVDRRALPAPDLSRPDI
                     ESDYVAPRTPLERQVAAAWAEVLGVDRVGIHDSFWELGGHSLLATKALSRLYDSLGVD
                     LPLQTLFEAPTLAGFTACLAHSVIADSGEEAEQFLEELAAMSEDEVRDLLGEDW"
BASE COUNT         1233 a         2897 c         2904 g         1280 t
ORIGIN      
        1 cctcgcgcgg ccggttcatc gccagattga cgcggccgat gccgttgaga gtgataccct
       61 cgccacggat ctcgcgccac cgccgccgaa gagcgagcgc ctcctcgtag cgttccagag
      121 cctcctccag ccttccgatg ccgagcaggg cgtcaccctg cttggagagg atgaccgcct
      181 tctgcaaggg gtccggctgc cgctcgagaa aggcgagggc tcggcggtac tgctccaggg
      241 ccagacggct ttctcccaga gagacgtaga ggactccgag attcgcgtgg gtcgtggcga
      301 ggtcacccca ctccctcagc tcatgccaga tctccaccgc gcgggagaag aggtcgatgg
      361 ccgtgtgagt ccgcccctgg taccggcgaa cgatggccag gctgttcaag gtcgtcgcct
      421 gctcgaagcg gttgccctgc tgctcccagg atgtcagagc ctgctcgtac gtgccagcgg
      481 cgctgtcgag gtctccgcga caggccctgg cctcccccag gtagtacagc gagagcgcct
      541 gctgcttcgg cctgctcacc tcgccgaaga gctgaacggc ccgctccaac gccgcggtcg
      601 cctcacagca gcggccggaa tcgtcactgt acatcttgcc cagccgatag agcgcccagg
      661 cctcccgctc ctcgtccccc accttgcgcc agagcctggc cgcatccctg taggctgcag
      721 ctgccgcttc cggggagccg gtctcctcgc gctggatcag gcgggccatg gaaaaagctg
      781 ccgcggcggc ggcgcgatcc cggtcctcat cggtagccac ccgcagggcc tcgatccgga
      841 tctcgtaacg tcccccgccg gctccttcgg gagaggcgac ctccaggagg taggggccgg
      901 cggagcccgt agccaggaac agatcctcgg gtcccttgtc gtccgttggg ctgtcgacgc
      961 gcaacaggcg ctgccccgac gggtcccgga tcgtagcagc gatgtcgacg ccgaattgct
     1021 cgacgaccag gtggaggtat tggtccttgg aaagcgggag ggtgtagcgc cgggtctcgc
     1081 caggcggcgg atgatgtcgc tgccgcccac cgttcgtcag aaccttcagc gccaggggca
     1141 cgggcggggc gggagccgcg agagaggcgg gacgcggctc ggcagcggcg gacggacggc
     1201 tgcggagagt ccgctttgga cccggccggc tcacgcccca ccccacggcc gcgagtgtcc
     1261 ccagagtcag gatgaggagc gcgaccgcga cgcggtcgtg agtcacccct ctacgtctca
     1321 gggatcgccc gccccgcaag cttcctcctc gagatccata ttgttgccac gaagttactt
     1381 catcgtagca gacgatgggg aatcgtcaag gagctgccgg gcgcacccgt agcgcgaact
     1441 cctccacgat ccggcgctcg ccggacgtgc tctcctcgag caggacgcgg tagctccccg
     1501 cctccagcgc tccgcgaggg atctcgagac ggaagttgcc ggcctcgctg ctcgggatgt
     1561 cctccaggcg gatcaccacg gctccatccg gagcgaccac ctcgatgccg tacgtcgaag
     1621 ccgccagctc cgccgatgga ttgaggatca tccagatccg ctccccctgg gccgggagcc
     1681 gaagctcggg cgcctcgcgg agcccttgcc gcaccgagtc gaccggctcg aggttgacca
     1741 atggaggatt ggcctgtggc tgctggagca atgcgatggt ccgccgctgg tccagggcca
     1801 ccacggcgag cagcgccgcg gccagcccca ggacggcggc cgtcgcccag gcccggcgcg
     1861 gcggagccgg ccggtgccac gggcgctccg gcggagccgc cggccggtcc gggaggcgcc
     1921 gtgcccgctg ccgctgccag gccgcatcga gctcggcagg ggagatctcg gaggtcgcgt
     1981 cgtcttctcc ttcgaggagg gcagccagat cgaggagctc ggcggtgcaa tccgggcagg
     2041 ccaccaggtg cgcgcggatc tcctccgcct cgtccggcac caggcggcac tcgtgatagg
     2101 ccacgatgcg ctcgggagtc gggtgctggt cgatggcgcg tctctccctc ttgaaatggg
     2161 cccggagtcg ttcggcgggc tcagcgctgg acatctgcgt cctccatggc ttcgcccgtc
     2221 agccacagcg agcccgggcc aagcctctgc ctagggagaa gccgccaccg cggcctggtt
     2281 tagccattca ctccacccac tcgccatact cttcgcccag ctccgctcgc aggcgttgcc
     2341 gcgcctggaa gaggtgcgcc ttgaccgtct cgatggagat atggagcacc tctgcaatct
     2401 cacggtactt caggtcctga tcaatgcgca aggtgagcac ttgccgcatc tgcggcggca
     2461 gccgtttgag ggcctgtgcc agggcctccc ggcgctctct ctcgtaggct tcccgggcgg
     2521 gcgagtctcc gggcgccacc ggctccggcg cgaccgggtc ctcctcggtc gccggggtga
     2581 tggagacctc cgtcgcgttg cgcttctccc gatggcgcca gcggtacttc ctgcggtgga
     2641 ggttggccgc cacggcgaag acccaggact tgaaggggcc ctcgtggcga tacgaatcga
     2701 tccggtcgaa gacctggaac agggtctcct gcgccagatc ctcgcagtcc tggggagcgt
     2761 agccgcggtg tctgaagaag cgatggatcc acggatagaa gctgtcgaag atgcgacggc
     2821 tgttgagctc gacgcggatg cccgcctgga gctctgtcac cgcacgctgg aggtcaaggt
     2881 cgtcttcccg cccgggggcc atggctctct acccgctcga ggtctgtcca agtggatcag
     2941 cgcatcctat cgcaccgcgg cacgagagat gcaaaagggg tccgaccggg ccgcggcact
     3001 tccatctgcc gttggcgctc gctatcctca cggtggccga aagccgcatg ccggagatcc
     3061 tggccgtgga gtgaggcggc cggacgccac taaacacgtt ccagggaggg tttgaatgcg
     3121 acttcgccga cgtcacgtcg ttccgctgtg cctcgtcttg gggcttctga tggctgacct
     3181 cgccgccgcc tccggcttcg gcctcttcca gcacgggggg cgggccatcg gccaggtcgg
     3241 cgcgctgacc gcccgcgcgc ccgatccgtc ggcgctcacc tacaacccgg cggcgatcac
     3301 ccagctcgaa gggctgcagg ccatggccgg cctcgacttc aacaacgccg aggacgccta
     3361 cgagagcccc accgggcgct tcgcggccga tcacatcatc cagttcccgc ccctcgccta
     3421 tctcacctgg aaacccaagg acagctcgtt cgccctggga atcgggctcg acgcgccgtt
     3481 ctggtatgtg gaggactggg agccggcgct gtttcccggc cgcttcatct cgcgccgctt
     3541 catgctgcgg gtctatgagc tccacccggt ggccgcctgg gagatgggcg gcggctggag
     3601 catcggcggc ggcgtccgct atctcttcgg ctcgatggag caggacgaca gcatcatctc
     3661 gcagttcttc atccccggca ccggcaacgt gccggtcgag ctcgagcgca acgccgacgc
     3721 cgacgtggac gacctcacct acgacctggc cctgcactac acgcagccgg catgggggtg
     3781 gggcgtggtc taccgcggcg ccgccaacct caaggggaac ggcgacctcg acttccagcc
     3841 ccgtaacgtg ccgaacatcc cggggctcca gacgcagatc gatcagcggt tcggttccgg
     3901 atcgtcccgc cagtcgttcg agatcccccg cgagctgcgc ggcggcatct gggtggcccc
     3961 ctaccccgag ctgcggctgg agctggacgc ctcgtggcag agctggtcga gcctggaggc
     4021 cacctccatc acctactcca accccatcgg cacactgtcc gaggagatcc cccgcgattg
     4081 ggacgacacc atcagcctgc ggctcggcgt cgagggggag atcaccgagg ccttcatgct
     4141 cttcggcggc gtcgcctacg agccctcgcc ggccaccggc gagaacgccg cgccggactt
     4201 cccgcgtagc gacgccatgg tctacgccgc cggcttcagc tacggcttcc cccagatctc
     4261 cttcgatgtc ggctactcgt tccacgagca caacagccgc agcgtcgagg gccaggagcc
     4321 gctgacgaac cccacccgtt ccggccgtta cacgtccagc gatcaggtgt ggggcttcgg
     4381 ggtgaggtgg aggttccagt agggagctag agaaaaggta gagaaaaaaa gggccggagg
     4441 cgaacctccg gccctttcgg tatccaggct ctctagaccg gcgccagctc gggctggtgg
     4501 gcggcgatct cccggaacat gccgggcgtg taggcggtga tgcggccggt gaaggcggag
     4561 gcggcgaccg tcagcggcga ggcgagccag agctggcccg gaccggagcg ccccttgtag
     4621 ttgcggttga tcgccgagac ggagacctgg tccggctcgt ccgagacgcc ggggccgcag
     4681 ccgatgcagg cgccgcagcc ggggtggatc agcgtcacgc cggcgcgctc gaaggtgtcg
     4741 gcgaggccgt tgcggcgcac gaagtcctcc accgccaccg agccgcactg gatcaggaac
     4801 ttcaccccgt ccgccacctg gaggccggcg tccaccgcct ccttcaccac ctggtgatag
     4861 aagatcaggt cgtcgatctt ccccgccgtg cagctaccgc cgtaggcgat gtcgatgggc
     4921 acgtcgccga tctcgtcgat ccaggcgccg ttggtggggt ccgacgggat cccctggtcg
     4981 gggttgcccg ggtgggcgac catcggccgc agcgccgaca ggtcgatcga gtggacgccg
     5041 ccgtcgtagt gggcgtcggg gtccggcgcc acagcgcgcg cccgcagttc ctcgacgtcc
     5101 accccgggcc gccactcggc gagccagcgg aaggtctcgt cgtcggcgat gacgatggcg
     5161 gtccgcgccg cgcactcggt cgccatgttg gtcagcgtcg cccgctcgtc catcgacagg
     5221 gtggcgacgc cgggaccggt gaactccatc acccggttga gggtgagctg cggccgggcg
     5281 tactcgagga ggatgtagag catcaggtcc ttggccgtca ccccgggctg gagcttgccg
     5341 gtgagctcga agcggatcga ctcggggacc tccacctggg tgaagcccga gtgcaccagg
     5401 tcggcgtact ccgtcgcccc cacgccccag gccagggcgt tgttcacccc gcccatgcag
     5461 gtgtggctgt ccgtcgcctg gatgaagtcg cccggatcga tcaggtgctc gcgcgccacc
     5521 tcgtggcaga tgcccggcga gatgccgtcc cgcgccgaga agtcgcgcac gccggtgtgg
     5581 cgctggaact cgcgctggag ctggcgcagg gtctcgatct tgtccgagaa ggcgcgcatc
     5641 ttcggcacct cgtcggcata gatcaggtgg tcctcgaaga ccgcgaactt gtccgggttg
     5701 cgcgcctcgt agtccggacc gtactcctgc tccaggaagt agtggacctg ggcggtggtg
     5761 aactcgtggg tgtagccgcc gtccacctgc accaccacgg cgtcgcccgg cttgacgaag
     5821 cggtgggcgt cgtccactcc cagcatgtgg tcggcgagga tcttctccgc catggtcatc
     5881 gggcgcggcc cggtgtcggg acggggcacc cccgcctcgc cggcggccac ggccttggag
     5941 aaggggaaga ggccgccgtg ctggatcacc agccggctga tcgggtccca cccctcgcag
     6001 aactcctcca gggggatgcc ctcgcctgtc tgcaggcgcc ggagctggtc gtggtcgccc
     6061 atgagcacgc cctggctgat gttgttgcgg gcgtggatcg gggcgaagga ggcggcgatg
     6121 gcgaggcgga tgccggacca cttctccgcc tgcaccgcgg tctcgcggct gctccccacc
     6181 cccttgcggt agcccgagac gatcacctcg aagttgccgt tgatcagggc gtcgcgctcg
     6241 aacaggcgct tgccgtcgac gatcaggccg gcataggcgt cgcgggcgat gtcctccggc
     6301 cggtggctga agcagaccca ggccggggtc atggcgtcgg tgttgatgtc gtccaggaga
     6361 tcctcgatcc tcaggtcttc catccggagg tccagctctc cacggagctg gcggcggatc
     6421 aggtccgggt ccttggtgag gaagaggacc cgcttgccag gggtcagcct gatgatctcg
     6481 ggcggtttca tcatccactc ctgtagggca gttcagagga aaggccaccc cggatacgga
     6541 ggggtgacgg ctccatgaca ttctaacctc gcctccaccg ccgcggcttc ccggcggcgg
     6601 agggagattg gagaagggct acggaaaagg gggggtcaac ccgagcggcg gccgtgccgg
     6661 gaggcgcggg acgccggggc ggcgcggcgg acaccggccc ggcgcagcgc ctcctgcacc
     6721 agccggtcgc cggcgaagtc ggggtcgccg tagcggcggt ggaggtggat cgcccggtcc
     6781 aggtgcccgg cggcctccgc gtactcgtgg aggtccatgg ccagggaggc gtcgtggccg
     6841 gcgagggccg cccgggccaa gggatcgccg gtgccgcggg cgagggcctc gcgggcgcag
     6901 tccagcgccc cccgcgcccc ctcccggtct ccctgcaggc gccgcgcctc gccgaccgcg
     6961 caccaggaca tgcccaggag gtccgccagc cgctcctggc cgtagtgctc ggtgtccagg
     7021 tgatgcgccg cctccagggc gagctcggcg atctccagcg cccgccgcgg gtcgctccgg
     7081 ccggccttgc ggctctcctc gagcagacgg tcgcagaacg cccaggagtg gtagcgccgg
     7141 tcgttgcgga cgcgcatccg ccgccgcccg gcatcgtggc ccaggagctc ggcccagagc
     7201 gccgcggcgg cggcatgctc gcgggagagg tccagcgcat gccggtccac ccgctcgaag
     7261 gccgccgtga aggcgcggtc gtaggcaccg gccggaggct ccacctgcgc agcagcggcg
     7321 ggcaccggct cctcccccgc gccctccgag gccctctccg gaacgcgcgc gagcaggcgg
     7381 cggatcatgt cgcgggcctc ttcgggcgag cgctcagacg gaaagagcaa tctgtctatc
     7441 ttcgatgaag ctgcgattgg agccatctca cacctccgga ccagcggtcc cccgggctcg
     7501 gcgcgaccac tgacacactg ccgctgaaaa aagaattgga aacaccggaa cggtgacggc
     7561 cggcgtcggt cttttttcac agcacgacgc gtgccacgga gggggcggcg cggtgcgccg
     7621 ctacccctct attctaagga gtgcaggaaa gggcggttcg cgggaccgat caccagtctt
     7681 cgcccagcag atcgcggacc tcgtcttcgg acatggccgc cagctcctcg aggaactgct
     7741 cggcctcctc gccgctgtcg gcgatgacgc tgtgggccag gcaggcggtg aagccggcca
     7801 gggtcggcgc ctcgaacagg gtctggagcg gcaggtccac ccccagggag tcgtacagcc
     7861 gggagagggc cttggtcgcc aggagcgaat ggccgcccag ctcccagaag ctgtcgtgga
     7921 tgcccacccg gtcgaccccc aggacctcgg cccaggccgc ggcgacctgg cgctccaggg
     7981 gggtgcgcgg cgcgacgtag tccgactcga tgtcggggcg ggacaggtcg ggggcgggca
     8041 gggcacgccg gtccaccttg ccgttcgggt tgagggggag ctcgtcgagg aagaggaagc
     8101 gggcggggat catgtagtgc ggcagccgct cgccgagcca ggcgcggagc gccgcggtgt
     8161 ccaccgccgg cagcgccggg gactcctgcg ggaggaggac gacgtaggcc accagccgcc
     8221 ggtcgccggc cacgtcctcg cgcgccatca ccagggcgcc ggccaccgcg gggtgggcca
     8281 tgagtgaagc ctcgatctcc cccggctcga tgcg
//
LOCUS       DASPJI010000028         4916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_288617, whole genome shotgun sequence.
ACCESSION   DASPJI010000028 DASPJI010000000
VERSION     DASPJI010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288617"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1353
                     /locus_tag="VEL74_00815"
     CDS             256..1353
                     /locus_tag="VEL74_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61094.1"
                     /translation="MHPALRAVVVPVLAGLLAGLAVPGSAWGVASFRLDSPPDCEEMS
                     LDLAALPGGGLVAVWAAGATGKPESSPRIFARLFDAHHAPRGSELQVNGETEALWPAV
                     AVGASGDILIVWVEAAPQRLVARRYDATGVAAGPEVVLSTAVSLGGAAVTATPTGFAV
                     VSAPGPEVRLWRLDFAGNPVGESEVVATMAGIPEAPFPVAQSPAVAYSPDGLVIAWAE
                     DHLAALTVFAVLRTTDNTHFLLSPQIHPAGHPQPAQPAVAVDGTGKILVAWNETPGRG
                     EAGSVRAQVFGRGGTFQGEAVYVDTQVGSELEVSAPEAAGLADGFIVAWESEDSLFPG
                     SVRHDVWLEGRAVPLTRDSGYFWSSARTTSR"
     gene            1350..1526
                     /locus_tag="VEL74_00820"
     CDS             1350..1526
                     /locus_tag="VEL74_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61095.1"
                     /translation="MMVKVLDGRAVNGRYWVFYASLSDVAYTLTVTDRVTGQVKVYDN
                     PAGRLASVADTEAF"
     gene            1549..3561
                     /locus_tag="VEL74_00825"
     CDS             1549..3561
                     /locus_tag="VEL74_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61096.1"
                     /translation="MNQPRLPILLATLVVCLLVGLDPLWGQHIRLDSRPDCQESSLDL
                     ANLPAGRLFAVWQQGAEASPGSPPRVYARIFDVNGAALAPEFPLTGENAVGPVVAASL
                     EGELLVVWGDAALGRLMARRFFANGTAAGPEIVVSSIFPSGGWAVTRTPNGYAVVAIQ
                     GSEVRLWRLGNTGSATGPTEVVASITGIPEDPTPIVRDPAVAYGPAGLVVAWAEEHFG
                     GASIGAVVRTATASLPLPLSAELQPADDPQPIEPAVAVDGQGRILLAWNESPLQEGTL
                     RAQIFGPDLVAAGPPFQVEDPGFPSLAFSAPDAAGLSDGFVVAWQFVPAYSGDAPHIT
                     LMRRIHGNGLVLGRATYLGGNSSTSRPVLIVDANQRATIGWPAGVRPPAGPAVELCFS
                     NGLYAVSADTAPRLLLLQDDRFEVSIEWEDFPRSAGQGRGVQLTGDSGYFWFFGENNI
                     EVVVKVLDGRAVNGHFWVFYASLTDVAYHLKVRDRATGHEKTYAKVGGRLASRADTLA
                     FPAGAPPAASAPLFAPPAMPATSETLATLETWAASEKSHTPPAEAVGPCAPASVPGPR
                     RVGLCLGERFEVEMDWQAVGTHGAGQGVQLTADSGYLWFFGPNNIEVMVKVLDGRAVN
                     GHFWVFLGGLSDVDYTIAVRDTVTGAVRTYVNPVGRLVSLADTAAF"
     gene            3573..4463
                     /locus_tag="VEL74_00830"
     CDS             3573..4463
                     /locus_tag="VEL74_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61097.1"
                     /translation="MRSSLVAKAVALLGLLALPALPARGQVWRLDTPPACQERAPEVL
                     AVGGGAFAAVWQGGAEPAAVGEPRIFVRLFDPHGVPRGPEFQVSGDYDAVLLDAAVNA
                     EGRFLVLWHDLLRGQLRSRAFSATGQPLGAEKVVFFDFVPPGVALTATNDGGFALVTA
                     AGLSVDLWRFDSLGQLHAGPPTEVESWASVAPEPPTFDGRFEARIEWTDHAGNTGEGR
                     AISLSNDSGYFWFFGPENVEVMVKVLDGRAVNGRFWIFYEALTDVEYTLLVRDTETDE
                     VKTYHNPAGQLRSHADTAAF"
     gene            4479..>4916
                     /locus_tag="VEL74_00835"
     CDS             4479..>4916
                     /locus_tag="VEL74_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61098.1"
                     /translation="MRFIYKVLVTACLLTIAAGHTSAGAQTFRLDTGAACQERRPDVA
                     ALPDGGFVAVWQQGEETDAVSRKRIYARLIKNDGTLPDPEFLVSAVDRGHSPQVAANG
                     EGRFLVVWLDEVNRVRYRPFGPTGASLGPDRPLPTNPFLTGFAL"
BASE COUNT          624 a         1794 c         1744 g          754 t
ORIGIN      
        1 cagtccgtgg gggtccgtga tcgtccgtgt tggtccgtgc tcgtccgtgt tggcgatccg
       61 cctcccccct ctcccgggga gggggcgggg aggcgggaga gggggccggg gggtgagggc
      121 tcctctatgt caccagagcg aaatggaagg cgcgctacgc gcactaggag agccagggtg
      181 aggactgttc tttgctacgc gatatgttat aggatcatat tcaagtagtt cccccttctc
      241 tccaaggagg catcgatgca cccagctctg cgcgctgtcg tcgtgccggt cctcgccggt
      301 ctgctggcag gccttgccgt gccgggcagc gcctggggcg tggcctcctt ccggctcgac
      361 tcgccacccg actgcgagga gatgtctctc gacctggcgg ccctgcccgg cggcgggctc
      421 gtcgcggtat gggcggcggg ggccaccggc aaacccgaga gctcgccgcg gatcttcgcc
      481 cggctgttcg acgcccacca cgctccccgc ggttccgagc tccaggtcaa cggcgaaacc
      541 gaggcactgt ggccggcggt ggccgtcggg gcctcggggg acatcctgat cgtctgggta
      601 gaggccgcgc cgcaacggct cgtggcgcgc cggtacgacg ccacgggcgt ggccgccgga
      661 ccggaggtcg tcctgtcgac ggccgtgtcg ctgggcggcg cggcggtgac cgcgacgccc
      721 accggcttcg ccgtggtgtc ggctccgggg cccgaggtgc gcctgtggcg cctcgacttc
      781 gcggggaacc ccgtgggaga gagcgaggtc gtcgccacca tggccggcat cccggaggcc
      841 ccgttccccg tggcgcagag cccggcggtg gcctacagcc ccgacggcct ggtgatcgcc
      901 tgggccgagg accacctcgc cgcgctcacc gtcttcgcgg tgctccggac caccgacaac
      961 acgcattttc tgctctcccc ccagatccac cccgcgggcc atccgcagcc ggcgcagccc
     1021 gcggtggcgg tggatgggac ggggaagatc ctggtggcct ggaacgagac gccggggcgg
     1081 ggggaggccg ggagcgtgcg ggcccaggtc ttcggccggg gaggcacctt ccagggcgag
     1141 gccgtctacg tcgataccca ggtcggcagc gagctcgaag tctcggcgcc cgaggccgcc
     1201 ggcctggccg acggcttcat cgtcgcctgg gagtccgagg attccctgtt ccccggctcc
     1261 gtccggcatg acgtctggct ggaggggcgg gcggtccccc tcacccgcga ctccggctac
     1321 ttctggtctt cggcgaggac aacgtcgagg tgatggtcaa ggtgctggac ggccgggcgg
     1381 tcaacggccg ctactgggtt ttctacgcgt cgctgtcgga cgtcgcctat acgctcaccg
     1441 tgacggaccg ggtcaccggc caggtgaagg tctacgacaa cccggccggc cggctcgcca
     1501 gcgtggcgga caccgaggcc ttctgacctg cgtgatgaga ggagagagat gaaccagccg
     1561 cgcctgccga tcctgctcgc gaccctcgta gtctgcctgc tcgtgggcct cgatcccctc
     1621 tggggccagc acatccggct cgactcccgt cccgactgcc aggagagctc cctcgatctg
     1681 gcgaacctgc ccgcgggccg gctcttcgcc gtctggcagc agggcgccga ggcctccccc
     1741 ggcagcccgc cgcgggtcta cgcccggatc ttcgacgtca acggcgccgc tctcgccccc
     1801 gaatttccgc tcaccggcga gaacgcggtc ggaccggtgg tggccgccag cctcgagggt
     1861 gagctcctgg tcgtctgggg ggacgccgcc ctggggcggc tcatggcgcg gcgcttcttc
     1921 gccaacggca ccgccgccgg accggagatc gtcgtgtcgt ccatcttccc gagcggcggc
     1981 tgggcggtga cccggacgcc gaacggctat gccgtggtgg cgatccaggg gagcgaggtg
     2041 cggctgtggc ggctcggcaa caccggctcg gccaccgggc cgaccgaggt cgtcgccagc
     2101 atcaccggca tccccgagga cccgaccccg atcgtgcgcg atcccgccgt cgcctacggc
     2161 cccgccggcc tggtggtggc ctgggccgag gagcacttcg gtggggcgtc catcggcgcg
     2221 gtggtgcgga cggccaccgc cagcctgccc ctgccgctct ccgccgagct ccagcctgcc
     2281 gacgacccgc agccgatcga gcccgcggtg gcggtggacg ggcaggggcg gatcctgttg
     2341 gcctggaacg agtcgccgct ccaggagggg acgctgcgcg cccagatctt cggccccgac
     2401 ctcgtcgcgg ccggcccccc cttccaggtg gaggacccgg gcttccccag cctcgccttc
     2461 tccgcgccgg acgccgccgg actgtccgac ggcttcgtgg tcgcctggca gttcgtcccc
     2521 gcgtactccg gcgacgcccc ccatatcacc ctgatgcgcc gcatccacgg caacggcctc
     2581 gtgctgggga gggccaccta cctgggtggc aattccagca cctcacgccc ggtgctcatc
     2641 gtcgacgcga accagcgcgc caccatcggc tggccggccg gggtgcggcc gccggccggg
     2701 ccggcggtgg agctctgctt cagcaacggc ctctacgcgg tgtcggccga caccgctccc
     2761 aggctgctcc tcctccagga cgaccgcttc gaggtgagca tcgagtggga agacttcccc
     2821 aggtccgccg gccaggggcg gggggtccag ctcaccggcg actccggcta cttctggttc
     2881 ttcggggaga acaacatcga ggtggtggtc aaggtgctgg acggccgggc ggtcaacggc
     2941 cacttctggg tcttctacgc gtcgctgacg gacgttgcct accacctcaa ggtgcgggac
     3001 cgggcgaccg gccacgagaa gacctacgcg aaggtgggcg gccggctcgc cagccgcgcc
     3061 gacaccctgg ccttccccgc cggggccccg ccggccgcct ccgcccccct cttcgcgccg
     3121 ccggcgatgc cggccacgtc ggagacgttg gcgacgttgg agacttgggc cgcctcggag
     3181 aagtcgcaca ccccgccggc cgaggcggtg gggccctgtg ctccggcgtc ggtgccgggg
     3241 cctcgccggg tgggcctctg cctcggtgag cgcttcgagg tcgagatgga ctggcaggcg
     3301 gtcggcaccc acggcgccgg gcagggggtg caactgaccg ccgacagcgg ctatctctgg
     3361 ttcttcgggc cgaacaacat cgaggtgatg gtcaaggtgc tggacggccg cgcggtcaac
     3421 ggccatttct gggtgttcct cggcgggctc tccgacgtcg actacaccat cgccgtacgc
     3481 gacaccgtga ccggcgcggt gcggacctac gtgaaccccg tcggccggct cgtcagcctg
     3541 gcggatacgg cagccttttg aagaggacac ccgtgcgctc cagcctcgtc gccaaggccg
     3601 tcgccctcct cggcctgctc gcgctgccgg ccctgccggc gcgcgggcag gtctggcgcc
     3661 tcgacacccc gccggcctgc caggagcgcg cgccggaggt gctggcggtc ggcggcggcg
     3721 ccttcgccgc cgtctggcag gggggagccg agccggccgc cgtgggggag ccgcggatct
     3781 tcgtccgcct cttcgatccc cacggcgtac cgcgcggccc cgaattccag gtgagcggcg
     3841 actacgacgc ggtcctcctg gacgccgcgg tcaacgccga gggccggttc ctggtgctct
     3901 ggcacgatct cctccggggg cagctccgga gccgggcctt cagcgccacc ggccagccgc
     3961 tcggcgccga gaaagtggtc tttttcgact tcgtgccgcc cggcgtggcg ctgaccgcca
     4021 cgaacgacgg cggcttcgcc ttggtgaccg cggccggcct ctccgtcgac ctgtggcgct
     4081 tcgactccct ggggcagctc cacgcgggcc ctcccacgga ggtggagtcc tgggcctccg
     4141 tcgctccgga gcctcccacg ttcgacggcc ggttcgaggc acgcatcgag tggaccgacc
     4201 acgccgggaa caccggggag gggcgggcga tctccctctc caacgactcc ggctacttct
     4261 ggtttttcgg ccccgagaac gtcgaggtga tggtcaaggt gctggacggc cgggcggtca
     4321 acggccgctt ctggatcttc tacgaggcgc tcaccgacgt cgagtacacg ctcctggtgc
     4381 gcgacaccga gaccgatgag gtaaagacgt accacaaccc ggccggccag cttcgcagcc
     4441 acgccgacac cgcggcattt tgagacagga ggatcttcat gcgcttcatc tacaaggtcc
     4501 tggtcaccgc ctgcctgctc acgatcgccg ccggccacac gtccgccggg gcgcagacct
     4561 tccggctcga caccggagcc gcctgtcagg agcggcggcc cgacgtcgcc gcgctgccgg
     4621 acggcgggtt cgtcgctgtc tggcagcagg gcgaagagac cgacgccgtc agccggaaac
     4681 ggatctacgc ccgcctcatc aagaacgacg ggacgctgcc cgatcccgag ttcctggtca
     4741 gcgccgtcga ccgcggccac tcgccgcagg tggcggccaa tggcgagggc cggttcctgg
     4801 tggtctggct ggacgaggtg aatcgggtcc gctaccggcc cttcggcccg acgggggcct
     4861 ctctcgggcc tgacaggccg ctccccacca accccttctt gaccggcttc gcgctg
//
LOCUS       DASPJI010000029        10396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_1643, whole genome shotgun sequence.
ACCESSION   DASPJI010000029 DASPJI010000000
VERSION     DASPJI010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10396)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10396
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1643"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VEL74_00840"
     CDS             complement(<1..725)
                     /locus_tag="VEL74_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61099.1"
                     /translation="MSPAAVARSAVRVLHPALDPLASAAPLVRLSDARLSDQQRIAVI
                     LQAAGLLSLLDRAGWQPPAEWPASPPVGVGPGGRLVVAGVAPGRSGRPTQELLRDLIT
                     QLFRGEEGETLPGRGAARRAARVLQDAWRQSLAPVPADEAVSQVLEAAPFLWEPAFAA
                     ARRALVGELWTPRDGGEDRPRLWVAGPPGWRARLLAGCGDLATLQSLIAGDEARALWQ
                     REEAGDPRDLASSGRWRAAVAAW"
     gene            complement(722..2071)
                     /gene="aroA"
                     /locus_tag="VEL74_00845"
     CDS             complement(722..2071)
                     /gene="aroA"
                     /locus_tag="VEL74_00845"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HYG61100.1"
                     /translation="MTASPSTSPDLRMIPEGRRAMGRVRVPPSKSLTHRYLNLALLAG
                     APMVLERPLDAEDTRLFLAALGRCGFQVERIGDEVRLTPGAGPAEAGPDGVKIFCGNA
                     GTMLRFLVASLTAVPGRWRLDGVPRLRERPVGPLVDALRRLGAGIEYLDRPGFVPLRI
                     QGGTLGSGTTTLAAGESSQYLSALLMAALRAPGEVVIEVESLTSQPYVDVTLAAVAEL
                     GGRIEPAVDLGHRAWRVLPSQPSSPLGAGRVRVEGDASAACYPAAAAALTGGVAVLEG
                     LSPASRQGDRRFLDLLAEMGAEVVAASGDLEVRGGSGGLRGIEADLSGMPDQVPTLAA
                     LAPFARGVTRIFNVAHLRIKESDRLAAMAGELRRLGAEVEEGPASLRIPGVWADHEPP
                     TDRVVVETHHDHRIAMSLALVGLRRPGVVVSSPGVVAKSYPEFWRDFEALLEAAPVP"
     gene            complement(2043..2810)
                     /locus_tag="VEL74_00850"
     CDS             complement(2043..2810)
                     /locus_tag="VEL74_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYG61101.1"
                     /translation="MSFRVTMLGSGTSTGVPVIGCTCKVCTSSEPRNRRWRTGLKVEL
                     ETGVVLVDAPTDLREQALRFGIPRVDAILFTHSHADHIFGLDDVRIFNFRQRAAIPCY
                     GSEETLRALRRTFAYVFEATQEGGGKPQLELLEVREPFRVLGQEVVPVPVWHGSMEVY
                     GYRIGRFAYVTDCNHIPEESFALLQGVEVLILDALRYRSHSTHFSVQEAVEAAARIGA
                     ARTVLTHLAHEIDYSAPDFPLPSGIEFGYDGLTFDIA"
     gene            2963..4327
                     /locus_tag="VEL74_00855"
     CDS             2963..4327
                     /locus_tag="VEL74_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HYG61102.1"
                     /translation="MNGAPFLLAASVPGGGLDIPPGWGLFLGFLLVVLNGFFVAAEFA
                     LVKVRPTQIDPYVQLGQRRAKVARHMIRHLDAYLSATQLGITLASLALGWIGEPAFAW
                     IVEPLVVPFVGEDSPLVHSLGLTVSFLVITILHIVLGELAPKSLAIRKSEATTLWVSL
                     PLFVFYKLTYPAIWLLNHTANALLKVVGIAPVSEGEMMHDEEELRLLLASSHASPLSL
                     QKRELLDNIFELSHRVARQIMLPRQDVVYLSTTRTLTENMRLARRSGHTRFPLCQGDL
                     DHVVGVIHIKDLFRRERPITSLEEVAREIAFVPETLTLDRLLKRMRTERFHLAAVIDE
                     YGGVSGVVTLEDVIEEIVGQIQDEFDVENPELLAQGDGVYEVSGGMLIEDLEAELEIE
                     LSDRDEDTIGGLVLSELGRNPAVGDRVELGPVIIEVLEVQFNRVQTVRVTVTSPATVP
                     PEEE"
     gene            complement(4290..5606)
                     /locus_tag="VEL74_00860"
     CDS             complement(4290..5606)
                     /locus_tag="VEL74_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61103.1"
                     /translation="MAPKPLLVLLCAGLLSLGAAPAPSPSPGLPVGAEPLPAALERRV
                     DELVRQTESIRGLRLARPVLRGVLEEEAVRRTLAEKIGQELPPEDLRSFELALEAFGL
                     IPGNLDLATYLPKLLSEEVAGFYDPEGKYLVLVRRAGQGADETDDSVLVHELAHAIQD
                     QHFRLDVYTDFDPLSDEGTARSAVVEGDAMWVMLRSIAGSDAAFDRLAGALSKDPEVL
                     VSQAAIGPGALAGAPPFLRESLLFSYLQGLTFCLRVKELGGRELLDRAFTTDPPRSSE
                     QILHPEKWAVKELRDDPVPVPVPDVGDALPGWTRAAEGQLGEMGIAVLLRGSAGERST
                     AEGAAAGWGGDRFAVYTRGEAPGRRLLIWSTEWDSEAEAAEFRSVAAARLGAGWEVRS
                     PAASPSRRVLVIRGDLDEAERGRVRSALDAPVAGATPPPAAPSQGR"
     gene            5713..6156
                     /locus_tag="VEL74_00865"
     CDS             5713..6156
                     /locus_tag="VEL74_00865"
                     /inference="COORDINATES: protein motif:HMM:NF021082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2383 domain-containing protein"
                     /protein_id="HYG61104.1"
                     /translation="MATMTDTVTTTLNSLLRGEMSAIETYRQAIEKLDNEVGAQDLHW
                     LRKEHRDAADALWHHVEQHGAKPSEDSGPWGAFAKAVEGTAKLFGNAAALKALKEGEE
                     HGLKDYEDALGGHELPADCQALLRGLLERQRAHLPVIDRLLEQHK"
     gene            6408..8261
                     /locus_tag="VEL74_00870"
     CDS             6408..8261
                     /locus_tag="VEL74_00870"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HYG61105.1"
                     /translation="MKRTLLLLSALVAGALFPGRLEGAATMPEPISYTLRFPAPQTHY
                     VEAEAWLPTGGRPAVDLAMAVWTPGSYMVREFSRQVEGFEARSEAGDPLPWDKTSKNH
                     WRVETRGLSRIAVRYRVYSREMSVRTNFVDAGFALLNGAATFLTLAEELGEDGQRAHE
                     VHLVLPPQWKVAVSALPELSAEGQERRYRAPDFDTLADSPLYAGNARVHRFEVGGVPH
                     LLVNEGEEEGGLWDGGAAARDAERIARQQAAFWGEVPYPRYVFFNLLTETGGGLEHRD
                     SSVLMASRWGLRTRDGRLDWLGLVSHELFHAWNGKRLRPVELGPFDYEREVYTRGLWF
                     VEGMTSYYDDLLVHRAGLATRKELLARLSRMIETLQTSPGRLVQPLDDSSFDAWIKFY
                     RRDENFTNTGTNYYTKGALLGFLLDARIRRATSGRRSLDDVLRLAYRRWSGERGFRPE
                     ELRALFDEVGGTGLGAWLDQALTTAAELDYGDALGWYGLRFAAEKKDENGGKLPAAWL
                     GAETEVKAGRLTVTEVKRGTPAHEAGLNVDDEILAIGEYRVPPEEWAKRLESYRPGEP
                     ASLLIARRERLLRLPVTFGEKPKPGWKLEVDPGATPEQKARLDAWLKGSAS"
     gene            complement(8265..9023)
                     /locus_tag="VEL74_00875"
     CDS             complement(8265..9023)
                     /locus_tag="VEL74_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HYG61106.1"
                     /translation="MTVPLTPFPERQAASGLALPAGEIHVWKVDLNPPAHRVEQLGRL
                     LAPDEWERANRFRFDRHRRQYAVGRGALRMLLGAYTGLPPQGVRFSYGPRGKPFLAPE
                     QAGDLQFNLSNSDEMALVGFVRGLEIGVDLEFMKPMSDLEQISERFFSVPERRVLRNL
                     PEEQKPEGFFNCWTRKEAYLKAVGEGLAVPLDSFDVTLAPGEPARMLAVEGDAERATR
                     WFYHHLRPAQDYIGAIALEDPEGREWRVSTWGFV"
     gene            complement(9031..9765)
                     /locus_tag="VEL74_00880"
     CDS             complement(9031..9765)
                     /locus_tag="VEL74_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017744596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase II family protein"
                     /protein_id="HYG61107.1"
                     /translation="MNPRARLRMFCFPYAGGGASAYRGWAASLPGEVEVCPVQLPGRE
                     SRLRDQPFTRAEVLIPAIADALAPYMDMPFVFFGHSMGGMISFELTRELRRRGKALPL
                     HIFVSGRRAPQVPAREEPIHDLPEPEFIEKLRELNGTPEEVLQHAELMKLLVPILRAD
                     FAVNETYTYTEEEPFDIGLSAFGGLGDVEVTKEDLELWRTQTRARFRLRMLPGDHFFL
                     NSGRDLLTEAIARDLGEVRGTGTLAW"
     gene            complement(9884..10129)
                     /locus_tag="VEL74_00885"
     CDS             complement(9884..10129)
                     /locus_tag="VEL74_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HYG61108.1"
                     /translation="MSWEDREDNTIYKVVINHEEQYSIWPAHRENPLGWNDVGKSGPK
                     QECLDYIKEVWTDMRPLSLRKKMEEMERNKQANQETN"
BASE COUNT         1609 a         3784 c         3548 g         1455 t
ORIGIN      
        1 tcccaggcgg ccacggcggc tctccagcgg ccagacgacg cgaggtcccg gggatctccg
       61 gcctcctccc gctgccatag ggcccgcgcc tcgtctcccg cgatgaggct ctggagagtt
      121 gccagatcac cgcagccggc gagcagacgg gctcgccaac ccggcgggcc cgccacccag
      181 agccgcgggc gatcctcacc tccatcgcga ggggtccaca gctcccccac cagcgcccgg
      241 cgcgcagccg cgaaggcggg ctcccagagg aagggggccg cctccagcac ctgggagact
      301 gcctcatccg cgggtaccgg cgccaatgac tgcctccacg cgtcctggag gacccgtgcc
      361 gcgcgccggg cagccccccg gcccggcaga gtctccccct cttcaccccg gaagagttgg
      421 gtgatgaggt cacgcaacag ctcctgggtt ggacggccgg agcgtcccgg cgcgacaccg
      481 gcgacgacca gccggccgcc gggtcccacc cctaccggcg gcgacgccgg ccattccgcc
      541 ggcggctgcc agccggcgcg gtccagcagg gagaggagcc ccgccgcctg cagaatgacg
      601 gcgatccgct gctggtcgct gagccgggcg tcactgagcc gaaccagagg tgcggcggaa
      661 gcaagtgggt cgagggcagg gtggaggacg cggaccgcac tacgggcgac cgcggccggg
      721 ctcatgggac cggagcggcc tccagcagcg cctcgaagtc gcgccagaac tcgggatagg
      781 acttggcgac cacgccgggg gaggagacca cgacacccgg ccggcggagc ccgaccaggg
      841 ccaggctcat ggcgatgcgg tgatcgtgat gggtctctac caccacgcgg tcagtggggg
      901 gctcgtggtc ggcccagacc ccggggatgc gcaggctggc gggcccctcc tccacctcgg
      961 cccccagccg ccgcagctcg cccgccatgg ccgcgaggcg gtcgctctcc ttgatccgta
     1021 gatgggcgac gttgaagatc cgggtgacac cgcgggcgaa gggggccagg gcggccaggg
     1081 tgggtacctg gtcgggcatg ccggagaggt cggcctcgat cccccgaagg cctccggatc
     1141 cgccgcgcac ctccaggtcg ccgctggcgg cgaccacctc ggctcccatt tcggccagca
     1201 ggtcgaggaa gcggcggtcc ccctgtcgcg aagccgggga gagcccttcg agcaccgcta
     1261 cgccaccggt gagtgccgcc gcggccgccg ggtagcaggc tgcggaggcg tccccctcga
     1321 cccgcacacg tccggctccc aggggcgacg acggctgcga gggcagaacc cgccaggcgc
     1381 ggtggccaag gtcgacggcc ggctcgatcc ggccgccgag ctcggccacc gccgcaagcg
     1441 tcacgtcgac gtagggttgc gaggtgagag actccacctc tatcactacc tcgccggggg
     1501 cgcgcagggc agccatgagg agggccgaga ggtactggct cgactccccc gccgccagcg
     1561 tcgtggtacc ggagcccagg gtcccacctt ggatccgcag agggacgaag ccaggccggt
     1621 ccaggtactc gatcccggcc cccaggcggc gcagggcgtc gaccagagga cctaccgggc
     1681 gttcccgcag gcgcggtacg ccgtccagac gccagcggcc cgggacagcc gtcagggagg
     1741 ccaccaggaa acgcagcatg gtgccggcgt tgccacagaa gatcttgacg ccgtccgggc
     1801 ccgcctcggc cggacccgcg cccggcgtca ggcgcacctc gtcgccgatc ctctccacct
     1861 gaaagccgca gcggcccagg gcggcgagga agaggcgcgt gtcctcggcg tccagggggc
     1921 gctccagcac catcggcgcg ccggccagca gggcgaggtt gaggtagcga tgggtgaggc
     1981 tcttcgacgg cggcacccgc acgcgcccca tggcccggcg cccctcgggg atcatccgga
     2041 ggtcaggcga tgtcgaaggt gaggccgtca tagccgaact ctatccccga tggcagcgga
     2101 aagtccgggg cggagtagtc gatctcgtgc gccaggtggg tgaggaccgt gcgggccgca
     2161 ccgatgcgcg ccgccgcctc caccgcctcc tggaccgaga agtgggtcga atggctgcgg
     2221 tagcgcagcg cgtcgagaat cagcacctcc accccctgga gcaaggcgaa gctctcctcg
     2281 gggatgtggt tgcagtcggt gacgtaggcg aaccgcccga tacggtagcc gtagacctcc
     2341 atcgagccgt gccagaccgg caccggcacc acctcctgcc cgagcacccg gaacggctcc
     2401 cgcacctcca gcaactcgag ctgcggcttg cccccccctt cctgggtggc ctcgaagaca
     2461 taggcgaacg tccggcgaag ggcccgcagc gtctcctccg agccgtagca gggaatggcc
     2521 gccctctgcc ggaagttgaa gatgcgcacg tcgtcgagcc cgaagatgtg gtcggcgtgc
     2581 gagtgggtga agaggatggc gtccacccgc gggatgccga agcgcagcgc ctgctcccgc
     2641 aggtccgtgg gagcgtccac caggacgacg ccggtctcca gctcgacctt gaggccggtg
     2701 cgccaccgcc ggttgcgcgg ctcgctggag gtgcagacct tgcaggtgca gccgatgacc
     2761 ggtacgccgg tggaggtccc gctgcccagc atggtgacgc ggaaggacat gacggccagg
     2821 aagtgtagcc catgggaagg cccccggacc gttgtgctaa gatggtagac gcattgaatc
     2881 aagaccctcc cagtccatac gccacccgtc tgttccccgg ccaagaccaa caccctccgg
     2941 cagttgaggg ggaggcaccg ctttgaacgg cgcgccgttc ctgctcgccg cgtcggtgcc
     3001 gggaggaggg ctcgacatcc cgccgggctg ggggctgttc ctcggcttcc tgctggtggt
     3061 gctcaacggc ttcttcgtcg ccgccgagtt cgccctggtc aaggtccgcc cgacccagat
     3121 cgacccctat gtgcagttgg ggcagcggcg ggccaaggtg gcgcggcaca tgatccgcca
     3181 cctcgacgcc tacctgtccg cgacccagct cgggatcacc ctggcgagcc tcgccctcgg
     3241 ctggatcggc gagccggcct tcgcctggat cgtcgagccg ctggtggtgc cgttcgtcgg
     3301 tgaggactcg ccgctggtgc actccctggg gctgacggtg tcgttcctgg tgatcaccat
     3361 cctccatatc gtgctcggcg agctcgcccc gaaatcgctg gcgatccgca agtcggaggc
     3421 caccaccctc tgggtgtcgc tgccgctctt cgtcttctat aagctcacct acccggcgat
     3481 ctggctgctc aaccacaccg ccaacgccct gctcaaggtc gtcggcatcg ccccggtgag
     3541 tgaaggggag atgatgcacg atgaagagga gctcaggctg ctcctcgcct ccagccacgc
     3601 cagccccctg tcgctccaga agcgggagct gctggacaac atcttcgagc tgtcgcaccg
     3661 ggtggcccgg cagatcatgc tgccgcgcca ggacgtggtc tatctctcga ccacccgcac
     3721 cctgaccgag aacatgcgcc tcgcccgccg cagcggccac acccgcttcc cgctctgcca
     3781 gggggacctg gaccacgtcg tgggggtgat ccacatcaag gacctcttcc ggcgcgagcg
     3841 acccatcacc tccctggagg aggtggcgcg ggagatcgcc ttcgtgcccg agaccctcac
     3901 gctcgaccgc ctcctgaagc gcatgcgcac cgagcgcttc cacctcgccg cggtaatcga
     3961 cgagtacggc ggcgtctccg gcgtcgtcac cctggaggac gtcatcgagg agatcgtcgg
     4021 ccagatccag gacgagttcg acgtcgagaa ccccgagctg ctcgcccagg gagacggcgt
     4081 ctacgaggtc tccggcggca tgctgatcga ggatctggaa gcggagctgg agatcgagct
     4141 gagcgaccgc gacgaggaca cgatcggcgg tctcgtcctg tcggagctcg gccgcaaccc
     4201 ggcggtgggc gaccgggtcg agctgggacc ggtgatcatc gaggtgctgg aggtccagtt
     4261 caaccgtgtc cagaccgtcc gcgtgaccgt cacctcccct gcgacggtgc cgccggagga
     4321 ggagtagcgc cggccaccgg cgcgtcgagc gcgctccgta cccgtcccct ctccgcctcg
     4381 tccaagtccc cccggatcac caatacacgt ctggaaggtg aagccgccgg gctccggacc
     4441 tcccagcccg cgccgagccg cgccgcggcg acgctccgga actccgcggc ttcggcttcg
     4501 ctgtcccact cggtgctcca gatcagcagg cggcgtccgg gagcctctcc ccgggtgtag
     4561 acggcgaagc ggtcgccgcc ccagccggcc gcggcgcctt cggccgtgct cctctctcca
     4621 gcagagccgc gcagcaggac cgcgatcccc atctccccga gctgcccctc ggcggcccgg
     4681 gtccagccgg gcagggcatc gccaacatcg ggcacgggga ccggcaccgg gtcgtcccgg
     4741 agctctttca ccgcccattt ctcggggtgc aggatctgct ccgaggagcg cggcggatcg
     4801 gtggtgaagg cccggtcgag gagctcccgg ccccccagct ccttcacccg cagacagaag
     4861 gtgaggccct ggagatagct gaatagcaag ctctcgcgca gaaacggggg agcccccgcc
     4921 agggcgcccg gcccgatcgc ggcctgggag acgagcacct ccgggtcctt cgacagcgcg
     4981 cccgccaggc ggtcgaacgc cgcgtcgctc ccggcgatgg agcggagcat gacccacatg
     5041 gcatccccct ccaccaccgc cgaccgcgcc gtgccctcgt cggagagcgg atcgaagtcg
     5101 gtgtagacgt ccagccggaa atgctggtcc tggatcgcgt gcgccagctc atggacgagc
     5161 accgaatcgt ccgtctcgtc agcgccttgc ccggctctcc ggacgaggac caggtacttc
     5221 ccctccgggt cgtagaagcc cgccacctcc tcgctcagca gcttggggag gtaggtggcg
     5281 aggtccaggt tgccggggat gaggccgaaa gcctccagcg ccagctcgaa ggagcggaga
     5341 tcctccggcg gcagctcctg gccgatcttc tccgccagcg tccgccgcac cgcctcctcc
     5401 tccagcacgc cgcggagcac gggccgcgcg agccgcagcc cgcggatcga ctcggtctgc
     5461 cgcaccagct cgtccacccg gcgctccaga gcggccggca ggggctccgc gcccaccggg
     5521 agcccgggag agggcgacgg ggccggggcc gcccccaggg agagcaggcc ggcgcaaagc
     5581 aggaccagca ggggcttcgg cgccatggcg gccatcttaa cagcccgccc aaggcacgtc
     5641 ccttgcactt ttccaggtca cggtccgccg tggaccctgg agccgaaaac caacgcgagt
     5701 ggaggattgc cgatggcgac catgaccgat accgtgacga cgaccctcaa cagcctgctc
     5761 cgtggcgaga tgtccgccat cgagacctac cgtcaggcga tcgagaagct ggacaacgaa
     5821 gtgggcgccc aggacctgca ctggctccgg aaagagcatc gcgatgcggc cgacgccctc
     5881 tggcaccacg tcgagcagca cggtgccaag ccctccgagg actccggccc ctggggcgcc
     5941 ttcgccaagg cggtggaagg gacggccaag ctcttcggca acgccgcggc gctcaaggcc
     6001 ctcaaggaag gggaggagca cggcctcaag gactacgagg acgccctggg cggccacgag
     6061 ctcccggccg actgccaggc gctgctccgc ggcctgctcg agcggcagcg ggcacatctg
     6121 ccggtgatcg accggctgct cgagcagcac aagtagggcc tgccttccgg gaggggggcc
     6181 agcccccctc cgccgtccct tgatcctcgc gccctgtggg gcctgccgcc ggccctgtag
     6241 ggcaccgtca ccgccccctg gggccaaccc ctcgtgaaca aggctttcca gcgggagcgc
     6301 cggccacggt tgcctgcatc gctcatgcac gggcagtata tgtctccacc caggctgcag
     6361 ggagaaggat cgccttgacg tggcaacaca cttgaggaag taaactgatg aaacgaactc
     6421 tactcttgtt gtctgccctg gtggcgggag cgctcttccc tggccgcctg gagggagccg
     6481 ccacgatgcc cgagccgatc tcctacaccc tgcgcttccc cgccccccag acccactacg
     6541 tggaggccga ggcctggctc cccaccggcg gccgccccgc cgtcgacctg gccatggcgg
     6601 tctggacccc gggatcgtac atggtgcggg agttctcccg tcaggtggag ggcttcgagg
     6661 cgcggagcga ggcgggcgat cccctcccct gggacaagac gagcaagaac cactggcggg
     6721 tcgagacccg cggcctctcc cggatcgccg tccgctaccg ggtctactcg cgcgagatgt
     6781 cggtgcggac caacttcgtc gacgccggct tcgccctgct caacggcgcc gccaccttcc
     6841 tcaccctcgc cgaagagctg ggggaggacg gccagcgcgc ccatgaggtc cacctggtgc
     6901 tcccgccgca gtggaaggtc gcggtcagcg cgctgccgga gctgtcggcc gaaggccagg
     6961 agcgccgcta ccgggccccg gacttcgaca ctctggccga ctcccccctc tacgccggca
     7021 acgccagagt ccaccgcttc gaggtcggcg gcgtcccaca cctgctcgtc aacgaggggg
     7081 aggaggaggg gggcctctgg gacggcggcg ccgccgcccg ggacgccgag cgcatcgcgc
     7141 gccagcaggc ggcgttctgg ggggaggtcc cctacccgcg ctacgtcttc ttcaacctgc
     7201 tcaccgagac cggcggcggg ctggagcacc gggactcctc ggtgctcatg gccagccgct
     7261 ggggcctgcg cacccgcgac ggccggctcg actggctcgg cctggtgagc cacgagctgt
     7321 tccacgcctg gaacggcaag cgcctgcggc cggtggagct cggccccttc gactacgagc
     7381 gcgaggtcta cacccgcggc ctctggttcg tcgaggggat gacctcctac tatgacgacc
     7441 tgctggtcca ccgcgccggc ctggccaccc gcaaggagct cctcgcccgc ctgtcgcgca
     7501 tgatcgagac gctacagacc tccccgggac gcctggtgca gccgctggac gactcctcgt
     7561 tcgacgcctg gatcaagttc tatcgccggg acgaaaactt caccaacacg ggcaccaact
     7621 actacaccaa gggagcgttg ctcggcttcc tcctggacgc ccgcatccgc cgcgccacct
     7681 ccggccgccg cagcctggac gacgtcctgc gcctcgccta tcgccgctgg tccggcgagc
     7741 gcggcttccg tcccgaggag ctgcgcgccc tgttcgacga ggtggggggc accgggctgg
     7801 gggcctggct cgaccaggcg ctcaccacgg cggccgagct cgactacggc gacgccctgg
     7861 gctggtacgg cctgcgcttc gccgccgaga agaaggacga gaacggcggc aagctccccg
     7921 ccgcctggct gggggcggag accgaggtca aggccgggcg gctgaccgtc accgaggtga
     7981 agcgcggcac ccccgcccac gaggccggcc tcaacgtcga cgacgagatc ctcgccatcg
     8041 gcgaataccg ggtgccgccg gaggagtggg cgaagcgcct ggagtcgtac cgtcccggcg
     8101 agccggccag cctcctgatc gcccggcgcg agcgcctgct ccgcctcccc gtcaccttcg
     8161 gggagaagcc gaagccgggc tggaagctgg aggtcgaccc cggagccacc ccggagcaaa
     8221 aggcacggct ggacgcctgg ttgaagggga gcgcgagctg aaccttacac gaacccccag
     8281 gtgctcaccc gccactccct cccctcgggg tcttcgaggg cgatggcgcc gatgtagtcc
     8341 tgggcgggac ggagatggtg atagaaccag cgggtcgccc gctcggcgtc cccttcgacg
     8401 gcgagcatcc gcgccggctc acccggagcc agcgtgacgt cgaaggagtc gagcggcacg
     8461 gcgagcccct cccccaccgc cttgaggtag gcctccttcc gcgtccagca gttgaagaac
     8521 ccctccggct tctgctcctc cggcaggttc cgcaacaccc tgcgctcggg gaccgagaag
     8581 aagcgctcgg agatctgctc caggtcgctc atcggcttca tgaactccag atcgacgccg
     8641 atctcgagcc cgcggacgaa ccccaccaag gccatttcgt cggagttgga gaggttgaac
     8701 tggaggtcgc cggcctgctc gggggcgagg aacggcttgc cgcgcggccc ataggagaag
     8761 cggaccccct gcggcggcag ccccgtgtac gcccccagca gcatccgcag ggcgccccgg
     8821 ccgaccgcgt actggcggcg gtggcggtcg aagcggaagc ggttggcccg ctcccactcg
     8881 tcgggcgcga gcagccgccc gagctgctcg acccggtggg ccggcgggtt caggtccacc
     8941 ttccagacgt ggatctcccc cgcggggagc gccagcccgg aagcggcctg gcgctccggg
     9001 aacggggtca acgggacggt caccaggctc ttaccaggcc agggtacccg tgccgcgcac
     9061 ctcgccgaga tcccgggcga tggcctcggt gaggagatcc ctgccgctgt tgaggaagaa
     9121 gtggtctccg ggcagcatgc gcaaccggaa ccgcgcccgg gtctgcgtcc gccacagctc
     9181 cagatcctcc ttggtgacct cgacgtcgcc gaggccgccg aaggcggaga ggccgatgtc
     9241 gaagggctct tcctcggtgt aggtgtaggt ctcgttgacc gcgaagtccg cccgcaggat
     9301 gggcaccagc agcttcatca gctcggcatg ctggagcacc tcctcgggag tgccgttgag
     9361 ctcccgaagc ttctcgatga actcgggctc cggcaggtcg tggatcggct cctcgcgcgc
     9421 cggcacctgc ggcgcgcggc ggccggagac gaagatatgc aggggcagcg ccttgccccg
     9481 gcggcgcagc tcgcgggtga gctcgaagct gatcatgccg cccatgctgt gcccgaagaa
     9541 cacgaacggc atgtccatgt agggtgccag cgcgtcggcg atggccggga tcagaacctc
     9601 ggcccgggtg aagggctgat cccgcagccg gctctcccgt cccgggagct gcaccgggca
     9661 tacctcgacc tcgccgggca gggaggccgc ccagccccga taggccgagg cgccgccgcc
     9721 ggcataggga aaacagaaca tgcgcaggcg cgcacgcggg ttcacctctc ggtaggcgag
     9781 ccaccgatcg gcagaagcac tgctcggttt cacggtcgga tcgactccag gcggcgccgc
     9841 ggacccggga cggaagcccg ggaccaccac ggcaggcgcg agatcagttg gtctcctggt
     9901 tcgcctgctt gttgcgctcc atctcctcca tcttcttgcg aagcgagagc gggcgcatgt
     9961 cggtccagac ctccttgatg tagtccaggc actcctgctt cgggccgctc ttgccgacgt
    10021 cgttccagcc cagcgggttc tcccggtgcg ccggccagat ggagtactgc tcctcgtggt
    10081 tgatcacaac cttgtagatc gtattgtctt cgcggtcttc ccagctcatg acatctctcc
    10141 ttcagcgttc gatgaattcg gttcgccagc ctgcccgggg tttgagcctg cggtccacaa
    10201 aacgggcgag ctgtcctctc ccttaagtgc cgccaccgcc cgatgcgtga cacatcctcc
    10261 cggtttcagc tcggcgggag cctcagagct taccagctcc gccgcgtctg cggctagccc
    10321 ggccggaccc gatcccgtgc ccggccgagc gtcgcgatca gggcgtcgac gtcctcgggg
    10381 ccggtgcggt ggttgg
//
LOCUS       DASPJI010000030         3814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_573647, whole genome shotgun sequence.
ACCESSION   DASPJI010000030 DASPJI010000000
VERSION     DASPJI010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573647"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="VEL74_00890"
     CDS             <1..1282
                     /locus_tag="VEL74_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61109.1"
                     /translation="LGGPYAFFLGVVLGLIGLLAAQGDWSRRLRHLLLALLVVIPLGG
                     FFYLRNMALGVGPVAAKCEGVEDIRAQREAENQPAGPAFPRLFSVARQLGPMLRDWTL
                     VDAFFGVTPPPMREMGTGPVAPLLLLGGLLFPLALSRERRRAAWAVHSQIVLQLAFWL
                     TVPGAPPGHVFANIRYLIPALGFALAGGIAALERRQAGDRWIQGLALAVAAQGLLQLH
                     AEMPRGVRIVAALLLLATGALAFWPGLRAGLARRSRLLAVAGAAALVVGAPFLARFRT
                     VDRGRAFAQELTVHSTLASDHARAWEWLDANGGDGTVTVVGSPRTRFVYPAMGPRLER
                     RTAYVNINERDLRNAGDYPRCDPRVDPSFDAWLRHLAAQEVRWLHTSRRPNAPFPGEA
                     GWAESRPDLFALRYKDDTNRIYEFLPVARAAGSE"
     gene            1307..1543
                     /locus_tag="VEL74_00895"
     CDS             1307..1543
                     /locus_tag="VEL74_00895"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HYG61110.1"
                     /translation="MKRTNLLLDEQLLDEAIRLSGEKSSSQAVSRALEDFVRRHRARG
                     ILELAGSGLWEGDLSEMRDDLPARTSTRSRRDPG"
     gene            1530..1925
                     /locus_tag="VEL74_00900"
     CDS             1530..1925
                     /locus_tag="VEL74_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYG61111.1"
                     /translation="MILVDTSVWIEVFRKPSRIQLGAEIDLDEVVTCLPVIQEVLQGF
                     REDAAFRLARRSMFALPILESPLHQEVFEEAAQLYRAARRKGLTVRSAADCLIAACAL
                     RNGLTVLHHDRDFPKIARISPLQERDVLS"
     gene            2000..2881
                     /locus_tag="VEL74_00905"
     CDS             2000..2881
                     /locus_tag="VEL74_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYG61112.1"
                     /translation="MLKDLPAEDREERGRALPESSEFAALLAESSADFIGLCDMDFIP
                     VFVNQAGLRLVGLDSLEEAKHAEVRDFFFPEDQAFIVEEFLPRVLREGQGEVEVRFRN
                     FKSGAALWMIYRVHVLLQDGQAAGLATISRNITERRRAEEALRLSRYRLDMVVDSIQL
                     GLWYCDLPSRDLVWNSTHRQHLGLPPEVAPHADRFFEPLHPDDLESTREAVERSITEA
                     GDFDAEYRTIARDGCVRWIRRAEGHPVTLIALTGWGQEEDKRRSREAGFDHHLVKPVS
                     FDLLTQILAGQGAARLA"
     gene            complement(2896..3078)
                     /locus_tag="VEL74_00910"
     CDS             complement(2896..3078)
                     /locus_tag="VEL74_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61113.1"
                     /translation="MKKNPKKLRLNRDTLAVLAGAVSAPANGFQVAIDTSCGEVCSCP
                     ADTCNGDVLANFDALR"
BASE COUNT          539 a         1359 c         1297 g          619 t
ORIGIN      
        1 cctgggcggg ccctatgcct tcttcctggg cgtggtgctc ggcctcatcg gactgctggc
       61 ggcgcagggg gactggagcc ggcgcctgcg ccatcttctc ctggcgctgc tggtggtgat
      121 ccccctcggc ggcttcttct acctgcgcaa catggctctc ggggtgggcc cggtggccgc
      181 caagtgcgag ggggttgagg acatccgggc gcagcgggag gcggagaacc agcccgccgg
      241 cccggcgttc ccgcgcctct tctcggtcgc ccggcagctc ggccccatgc tgcgggactg
      301 gaccctggtg gacgccttct tcggggtgac gccgccgccg atgcgcgaga tggggaccgg
      361 cccggtggcg ccgctcctcc tcctcggcgg gctcctcttc cccctggcgc tgagccggga
      421 gcggcggcgg gcggcgtggg ccgtccacag ccagatcgtg ctgcagctcg ccttctggct
      481 caccgtgccg ggagcgccgc ccggccacgt cttcgccaac atccgctacc tcatccccgc
      541 gctcggcttc gccctggcgg gagggatcgc cgcgctggag cggaggcagg cgggggaccg
      601 ctggatccag gggctcgccc tggcggtggc cgcccagggc ctgctccagc tccatgccga
      661 gatgccgcgg ggcgtgcgga tcgtcgccgc gctgctgctg ctcgccaccg gcgccctcgc
      721 cttctggccc ggcctgcggg ccggtttggc gcggcgctcg cgcctcctgg ccgtcgccgg
      781 cgccgcggcg ctggtggtgg gtgccccgtt cctcgcccgc ttccgaaccg tcgatcgcgg
      841 ccgcgccttc gcccaggagc tcaccgtcca ttcgacgctc gcctccgacc acgcccgcgc
      901 ctgggagtgg ctggacgcga acggcggcga cggcaccgtg acggtggtgg gctcgccgcg
      961 cacccgcttc gtctacccgg ccatgggccc ccgcctggag cgccggacgg cctacgtcaa
     1021 catcaacgag agggacctga ggaatgccgg cgactacccg cgctgcgatc cgcgggtcga
     1081 tccctccttc gacgcctggc tccggcacct cgccgcccag gaggttcgct ggctccacac
     1141 cagccgccgc cccaacgccc ccttccccgg agaagccggc tgggccgagt cccgccccga
     1201 cctcttcgcc ctgcgctaca aggacgacac gaaccggatc tacgagttcc tgccggtggc
     1261 gcgggcggca gggagcgagt gaggaagctt gtggtacctt gaggccatga agcgcaccaa
     1321 cctcctcctg gacgagcaac tcctcgacga ggcgatccgc ctcagcggtg agaagtcgtc
     1381 ttcgcaggcg gtgtcccggg ctctggagga cttcgtgcgg cggcaccggg ccagagggat
     1441 cctcgagctc gccggctcgg gattgtggga aggcgacctt tcggagatga gggacgacct
     1501 gccggcccgt acctccaccc ggagccggcg tgatcctggt tgacacctcc gtctggatcg
     1561 aggtcttccg caaaccgagc cgtattcagc tcggggcaga gatcgacctg gacgaggtcg
     1621 tcacctgcct gcccgtcatc caggaagtcc tgcaaggatt tcgggaagac gccgcgttcc
     1681 gcttggcccg gagatcgatg ttcgcgctcc ccatcttgga gtctcccctc caccaggaag
     1741 tcttcgagga ggcggctcag ctctatcgcg ccgcccgccg aaaagggttg acggtgcgct
     1801 cggcggccga ttgcctgatc gcggcctgcg ctctccggaa cgggctgacc gtgcttcacc
     1861 acgaccgcga cttccccaag atcgcccgca tctcccccct ccaggaacgc gacgtcctct
     1921 cctgaagcgg ttgggtagcc cactttcctc caccaccagt acaatgcccc ttccgggccc
     1981 gcaaggccgg gtgagtggca tgttgaagga tctccccgca gaggatcgcg aagagagggg
     2041 cagggcgctt ccggagagct cggagttcgc cgccctcctt gccgagagca gcgccgactt
     2101 catcggcctg tgcgacatgg acttcatccc cgtcttcgtc aatcaggcgg ggcttcgcct
     2161 ggtcggcctc gacagcctcg aggaggcgaa gcacgccgag gtccgggact tcttcttccc
     2221 cgaggaccag gccttcatcg tcgaagagtt cttaccccgg gtgctgcgcg aggggcaggg
     2281 cgaggtcgag gtccgcttcc gcaacttcaa gagcggggcc gccctgtgga tgatctaccg
     2341 ggtgcacgtc ctgctgcagg acggccaggc ggccggcctc gccaccatca gccgcaacat
     2401 caccgagcgc cggcgcgccg aggaggccct gcggctcagc cgataccggc tcgacatggt
     2461 ggtggactcc atccagctcg gcctctggta ttgcgacctc ccctcccgcg acctggtgtg
     2521 gaactccacc caccggcagc acctcgggct gccgccggag gtcgcgccgc acgccgaccg
     2581 gttcttcgag cccctccatc cggacgacct ggagagcacc cgggaggccg tcgagcgctc
     2641 catcaccgag gccggcgact tcgatgccga gtaccgcacc atcgcccggg acggctgcgt
     2701 gcgctggatc cggcgagcgg agggccatcc ggtgacgctg atcgccctga ccggctgggg
     2761 ccaggaggaa gacaagcgcc gctcccggga ggccggcttc gaccatcacc tggtgaagcc
     2821 ggtgagcttc gacctcctga cccagatcct cgccgggcag ggagcggcgc ggttggcctg
     2881 agctcgtcgg cgcccctacc gcagggcgtc gaagttggcc aagacgtccc cgttgcaggt
     2941 gtcggccggg caactgcaga cctccccgca actggtgtcg atcgccacct ggaacccgtt
     3001 cgcgggagcg gagaccgctc cggcaaggac ggccagggtg tcgcggttga ggcgcagctt
     3061 cttgggattc ttcttcatgg ctcctcttct cctccacttg gaggcacgaa gtcgttcgga
     3121 cctccggatt tcatggtgct gagatggtag caccggactc cggcgtccca cgcggcgcct
     3181 gtcgttcctc agtgctgagg aacgcagccg gaggaccgaa gcggaggctc tttccttcgt
     3241 tccggccggg atggaaacgt tccggaccgg cgtccatcgc tcccgagcgt tccgggaagg
     3301 ctcccgggcg ccggggaatg atccggaggc ttcgggatca ccccgaacct tctgggatca
     3361 cggcgaagtc cctcgggacg attccggggc gcccggaatc gagccggagc gttcggacgg
     3421 ctcccgaagg gcttggagcg gctccgcagc ctccgcccag atccggaacg gcggggggtc
     3481 ctcatccgtc gtggagcaga ggctacaaaa gacacccgca tgatgtcaat ggaatcatcc
     3541 acatggtgtt gtgatactct tggcatccga gggattgcgg accaggctga tgcctgggca
     3601 gagatccctc cgctctttga caactgacgt acacgtccgg atgcggcgcc tggcgggggg
     3661 cgccgcgctc cggcgtgcgt tcgatccccg gtcgccccaa aagaagaggg cgccccgtgg
     3721 gacgcccgag gaaaggcccc aagggagggc cgcttcgctg tacttgtcta cgcacggggc
     3781 ggccggaggg tttcacaagg acgccctcga agac
//
LOCUS       DASPJI010000031         6022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_1793, whole genome shotgun sequence.
ACCESSION   DASPJI010000031 DASPJI010000000
VERSION     DASPJI010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6022)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VEL74_00915"
     CDS             <1..438
                     /locus_tag="VEL74_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase"
                     /protein_id="HYG61114.1"
                     /translation="TIPWGDVSTAWHTTGIPNIRVYTGVPPRQIRRMRFWSFVLPLAA
                     RPAIKRFLQRQVDRRAPGPSEQTRETARVHLWGEVRNAAGRTLTSTLETPEAYALTAV
                     SAVECAVRAAAGRVPPGAWTPARAFGAGFVAELPGVVAGELKG"
     gene            519..2159
                     /locus_tag="VEL74_00920"
     CDS             519..2159
                     /locus_tag="VEL74_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016862541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HYG61115.1"
                     /translation="MIDLWYKDAIIYSLDVDAFQDSNGDGIGDFRGLSERLDYLTSLG
                     VTCLWLLPFYPSPNRDNGYDVMDYYGVDPRLGTLGDFVEFMRRARERGIRVLIDLVVN
                     HTSDQHPWFQSARKDPASPYRDFYVWSETLPKNWNQGMVFPGYQDSIWAWDEEAGAYY
                     LHRFYAHQPDLNISNPLVREEICRIMGFWLELGVSGFRLDAAPFLIEMKGTDSAHPAD
                     NEDQYAYLREFRDFLSWRQGDAILLAEANISRDKVNQYFGDDARLHMLFAFLVNQQLF
                     LSLAKQETEPLVRGLRMLPVPPLTGQWANFLRNHDELDLGRLSEAERQKVFQEFGPDP
                     SMQLYHRGLRRRLPPMLGGDRHRVELAYSLMFALPGTPVVRYGEEIGMGEDLSLAERE
                     SVRTAMQWSDEEKGGFTSFTCPGRPVISGGEYGYEKVNVAAQQRDPKSLLHWMVRLIS
                     ARKQCPEVGWGSWEILEAGDPAVFALRYDWQDGTVITVHNLADREAAVRLHIDGGEPL
                     FELIGDRPYELIASDEEIALRPYGYRWFRLGGTRHFLL"
     gene            complement(2168..2464)
                     /locus_tag="VEL74_00925"
     CDS             complement(2168..2464)
                     /locus_tag="VEL74_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61116.1"
                     /translation="MFEIAALFLVLAVLGVAFVACALIAGLFEVVFKVALLPVMLAVG
                     LVKVVVVAVLALVGFVLMVTVGPVLVAIAIGLMVPVLVVGAVLWAGFGVLAAVF"
     gene            complement(2530..2922)
                     /locus_tag="VEL74_00930"
     CDS             complement(2530..2922)
                     /locus_tag="VEL74_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="involved in start site selection during the
                     initiation of translation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HYG61117.1"
                     /translation="MKSKTGKTVYSTESAKAPVREACRRCGTDPCRCEPRRSLPPSAQ
                     PVRVRRESGGRGGKLVTMAGPLVLVREEAAALLATWKKLLGTGGALTAVVARNGSPAF
                     ELEVQGDHADRILAELVKAGYPAKRSGG"
     gene            complement(3286..3525)
                     /locus_tag="VEL74_00935"
     CDS             complement(3286..3525)
                     /locus_tag="VEL74_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61118.1"
                     /translation="MSARKVTVEINEDLLSAVQRVHQIHYAPSPLDSPISPRYATCAV
                     NKRPHDVAVLLDAVGPACRERAGFFLHERLAVHRG"
     gene            3658..4293
                     /locus_tag="VEL74_00940"
     CDS             3658..4293
                     /locus_tag="VEL74_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HYG61119.1"
                     /translation="MARIEFTGNHEDLSTDKGYQFKFYCEKCGNGYMSSFKSSKLGMA
                     SSALSAAGSLFGGLFGQAANSAYEIQRAIGGPAHDAALKEAVEEIRPLFTQCTRCGDW
                     VCEPVCFNKKAMLCETCAPDLDEEIAAAQAEAAREQAFEKARSVDWMKGKNIGHVAGA
                     VCRSCGAKTGSAKFCPECGTPTTQKKSCMGCSHEVEGSPKFCPECGHKFAA"
     gene            complement(4355..4774)
                     /locus_tag="VEL74_00945"
     CDS             complement(4355..4774)
                     /locus_tag="VEL74_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HYG61120.1"
                     /translation="MSGLDRELLAEKAAVIERHLARVAEKLPDDPEDFLPATDASDAV
                     ILHLWQATQTTIDLAVSACIHFRLGTPGGYGTAFRRLADGGFLDSALADRLVRAAGFR
                     NVVAHAYDTLDMARVFRTAGEGPADLRAFLHALAERV"
     gene            complement(4771..5172)
                     /locus_tag="VEL74_00950"
     CDS             complement(4771..5172)
                     /locus_tag="VEL74_00950"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYG61121.1"
                     /translation="MKTSGTDQVLADVAALAREVPGLELLVLFGSRARGDSHPGSDWD
                     FGYLAGPSFDPDGLLARLVLLLDTDDVDLADLARANGLLRFRVAAEGRPLFESEPGRF
                     DDFSFWAISFWCDMGPIIREAYEGILEDLKA"
     gene            5237..>6022
                     /locus_tag="VEL74_00955"
     CDS             5237..>6022
                     /locus_tag="VEL74_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HYG61122.1"
                     /translation="MEDFLAARTQMAVSLMFHILFAIAGMAMPVLMVVSEWRWLRTRD
                     ELYLELTRRWAKGTAILFAVGAVSGTVLSFELGLLWPTFMEHAGPLIGMPFSLEGFAF
                     FTEAIFLGIYLYGWKRVPPRAHLLAGVVVAVSGGLSAVFVVTVNAWMNTPTGFKLVDG
                     RFVDIDPVAAMLNPAAFPQALHMLLAAYAAVGFAVAGIHAAVLLRHPGDRFHRAALAI
                     ALLVGGPAALLQPLSGDILAKMVADTQPAKLAAMEGHFETQQGA"
BASE COUNT          957 a         2129 c         1976 g          960 t
ORIGIN      
        1 accatcccct ggggggacgt ctcgaccgcc tggcacacga cggggatccc caacatccgg
       61 gtctacaccg gcgtgccgcc gcgccagatc cgccgcatgc gcttctggtc cttcgtcctc
      121 cccctggcgg cccggccggc catcaagcgc ttcctgcagc gccaggtgga ccgccgagcg
      181 ccgggccctt cggagcagac gcgggagacg gcgcgcgtcc atctctgggg cgaggtgagg
      241 aacgccgccg gacggacgct gacctccacc ctcgagaccc ccgaggccta cgcgctgacc
      301 gccgtctcgg ccgtggagtg cgccgtccgc gccgccgccg gccgcgtccc cccgggcgcc
      361 tggacccccg cccgcgcctt cggcgccggc ttcgtcgccg agctccccgg cgtggtggcc
      421 ggcgagctca agggataggc cttccgcggc tctcccccgc acctggcccg catcttgcct
      481 cgcaatcccc tggagcccaa gcccggggag ggagcgcgat gatcgacctc tggtacaagg
      541 acgccatcat ctactcgctg gacgtcgacg ccttccagga ctcgaacggc gacggcatcg
      601 gcgatttccg ggggctgtcc gagcggctcg actacctgac gagcctgggg gtgacctgcc
      661 tctggctcct ccccttctac ccctccccca accgcgacaa cggctacgac gtcatggatt
      721 actacggcgt cgacccgcgc ctggggaccc tgggcgactt cgtggagttc atgcgccggg
      781 cgcgggagcg gggcatccgg gtgctcatcg acctggtggt caatcacacc tccgaccagc
      841 acccctggtt ccagtccgcg cgcaaggacc cggcctcccc ctaccgcgac ttctacgtct
      901 ggtcggagac cctgccgaag aactggaacc aggggatggt cttccccggc taccaggact
      961 ccatctgggc ctgggacgag gaggccggcg cctactatct ccaccgcttc tatgcccacc
     1021 agcccgacct caacatctcc aaccccctgg tgcgcgagga gatctgccgc atcatgggct
     1081 tctggctgga gctgggggtg tcggggttcc ggctggacgc ggcgccgttc ctcatcgaga
     1141 tgaaggggac ggacagcgcc catccggcgg acaacgagga ccagtacgcc tacctgaggg
     1201 agttccggga cttcctctcc tggcgccagg gggacgccat cctcctggcc gaggccaaca
     1261 tctcgcgcga caaggtcaac cagtacttcg gcgacgacgc ccgcctccac atgctcttcg
     1321 ccttcctggt gaaccagcag ctcttcctgt cgctggcgaa gcaggagacg gagccgctcg
     1381 tccgcgggct gaggatgctg ccggtgccgc cgctcaccgg ccagtgggcg aacttcctgc
     1441 gcaaccacga cgagctcgac ctcgggcggc tgtccgaggc ggagcgccag aaggtcttcc
     1501 aggagttcgg gcccgacccc tccatgcagc tctaccaccg cgggctgcgc cggcggctcc
     1561 ccccgatgct cggcggcgac cgccaccgcg tcgagctggc ctacagtctg atgttcgcgc
     1621 tgccgggcac gccggtggtg cgctacggcg aggagatcgg catgggcgag gacctgtcgc
     1681 tcgccgagcg cgagagcgtg cgcacggcga tgcagtggtc ggacgaggaa aaaggcgggt
     1741 tcacctcgtt cacctgtccg gggcggccgg tgatctccgg gggagagtac ggctatgaga
     1801 aggtcaacgt cgccgcccag cagcgcgacc ccaagtcact cctccactgg atggtgcggc
     1861 tgatcagcgc ccgcaagcag tgccccgagg tcggctgggg gagctgggag atcctggaag
     1921 ccggcgatcc ggccgtcttc gctctccgct atgactggca ggacggcacg gtgatcacgg
     1981 tgcacaacct ggcggaccgg gaggcggccg tccggctgca catcgacggg ggcgagcccc
     2041 tcttcgagct catcggcgac cgcccctacg agctgatcgc cagcgacgag gagatcgccc
     2101 tccgtcccta cggctaccgc tggttccggc tgggaggcac gcggcacttc ctgctctgac
     2161 tacacaccta gaagaccgcg gccagtaccc cgaaccccgc ccacagcacg gccccgacca
     2221 ccagcacggg caccatcagc ccgatcgcga tcgccaccag caccggcccg acggtgacca
     2281 tcagcacgaa gccgacgagc gccaggaccg ccacgaccac gaccttgacc agccccaccg
     2341 ccagcatcac cggcaggagg gcgaccttga acaccacctc gaagagaccc gcgatcagag
     2401 cgcacgccac gaacgcgact cccaggactg cgaggaccag gaacagcgcg gcgatttcga
     2461 acatggcttg actacctccg cagctctcta cgggaggtag cgcgagaggg tttcagccgt
     2521 ggcggacgcc tatccgcccg agcgtttggc gggatagccg gccttgacca gctcggcgag
     2581 gatgcggtcg gcgtgatcgc cctggacctc caactcgaag gcgggcgagc cgttccgggc
     2641 gacgacggcg gtgagcgcgc cgccggtgcc gaggagcttc ttccacgtgg ccagcagcgc
     2701 cgccgcctcc tcgcgcacca ggaccagcgg ccccgccatc gtcaccagct tgccgccgcg
     2761 cccaccgctc tcccggcgga cccgcaccgg ctgggcggag ggaggaaggc tccgccgcgg
     2821 ctcgcagcgg cagggatcgg tgccgcaccg ccggcaagcc tcccgtaccg gggctttggc
     2881 agattcggtg gagtagacgg tttttccggt cttgctcttc atccgttgaa gagtactcca
     2941 gcttctccag ccaggatggc gaaggaaacg cgaggtcgat ccatactctt gaattccatg
     3001 aggtctcccg agcagggtcg attcatactt ctgaattcct tcgtcttacc ctcgctgggt
     3061 cgattcatac tcttgaattc cctccgctcc ccctcgcagg gtggacccat acttttggat
     3121 tccgttcgct cacccttgcc gggttgatcc atacttttga attccgtggc catactgggc
     3181 cttcccgtgg cttccccctt cgcttcctcc ggttttcctc cgaagggaac ggcgcaggac
     3241 atggagtcct ccgccggaag ggagagaagg aagagctcgc cgccgtcagc cgcggtgaac
     3301 cgcgagccgc tcgtggagga agaacccggc ccgctcgcgg caagccggcc caacggcatc
     3361 gaggaggacc gcgacgtcat gcggccgctt gtttacggcg caggtggcgt agcgcggcga
     3421 tatgggggag tcaagggggg atggtgcata atgaatctga tggacccgtt ggacggcgga
     3481 tagcaggtct tcgttgatct cgacagtgac tttccgggca ctcatggcgg cgactcgacc
     3541 tcctcgcggt gacgccctcc ggcggtgccc tgtggtacca ttccgcaggt tcttaccctt
     3601 tacagactct ggaatcaaac gtcccgatcg cctctgcgat caagaagggg tttctccatg
     3661 gctcgtatcg agttcaccgg gaatcacgag gatctctcga ccgacaaggg ttatcagttc
     3721 aagttctact gcgagaagtg tggcaacggc tacatgtcca gcttcaagtc gtccaagctc
     3781 gggatggcca gctcggcgct ctcggcggcg gggagcctgt tcggcggtct gttcgggcag
     3841 gccgccaaca gcgcctacga gatccagcgc gccatcggag ggccggccca cgacgccgcg
     3901 ctcaaggagg cggtcgagga gatccggccg ctgttcaccc agtgcacccg ttgcggcgac
     3961 tgggtctgcg agccggtctg cttcaacaag aaggccatgc tctgcgagac ctgcgcgccc
     4021 gacctcgacg aggagatcgc cgccgcccag gccgaggcgg cccgcgagca ggcgttcgag
     4081 aaggcgcgct ccgtcgactg gatgaaaggc aagaacatcg gccacgtcgc cggcgccgtc
     4141 tgccggagct gcggcgccaa gaccggctcc gccaagttct gtccggagtg cggcacgccc
     4201 acgacgcaga agaagtcctg catgggctgc agccacgagg tcgagggaag ccccaagttc
     4261 tgtccggagt gcggacacaa gttcgccgcc taggccctcc cccatcccga agcgccgtcc
     4321 cctcgccggg gcggcgcttt tgttttgggc ggcttcagac ccgttcggcg agagcgtgga
     4381 ggaacgcccg caggtcagcc ggcccctcgc cggcggtccg gaagaccctg gccatatcca
     4441 gcgtgtcgta ggcgtgggcg accacgttgc ggaagcctgc cgcgcgcacg agccggtcgg
     4501 ccagcgcaga atcgaggaag ccgccatccg cgaggcgtct gaatgccgtg ccgtatccgc
     4561 ctggagtgcc caagcggaaa tgaatgcagg ccgagacggc cagatcgata gtcgtctggg
     4621 tcgcctgcca gaggtggagg atcacggcgt ccgaggcgtc cgtggccggc aggaagtcct
     4681 ccggatcgtc cggcagcttc tccgccaccc gggccagatg acgctcgatg acggccgcct
     4741 tctccgccaa cagctcccga tccagcccgc tcacgccttc agatcctcca aaatcccctc
     4801 ataggcctca cggatgatgg gtcccatgtc gcaccagaaa gagatcgccc aaaaggagaa
     4861 gtcgtcgaag cggcccggtt ccgactcgaa gagcgggcgg ccttcggcgg cgacccggaa
     4921 acggaggagg ccgttggcgc gggcgaggtc ggcgaggtcc acgtcgtcgg tgtccaggag
     4981 gaggacgagc cgggccagca ggccgtccgg gtcgaaactc gggccagcca gatagccgaa
     5041 gtcccagtcc gaccccgggt ggctgtcgcc cctcgcccgg gagccgaaga ggacgagcag
     5101 ctccaggccg gggacctccc gtgccagagc ggcgacgtcc gcgaggacct ggtctgtgcc
     5161 cgacgttttc acgacgggga tagcctatcc cgaatcctcc accggggcgg cgcttttggc
     5221 ttatgctggc cgggcgatgg aagacttcct ggccgcccgc acccagatgg cggtctccct
     5281 gatgttccac atcctgttcg ccatcgccgg catggccatg ccggtcctga tggtggtctc
     5341 ggagtggcgg tggctgcgga cccgcgacga gctctacctg gagctgaccc gtcgctgggc
     5401 caaggggacc gccatcctgt tcgccgtcgg cgcggtgtcg ggtaccgtgc tctccttcga
     5461 gttggggctc ctctggccca cgttcatgga gcacgccggc ccgctcatcg gcatgccctt
     5521 ctccctcgaa ggcttcgcct tcttcaccga ggccatcttc ctcggcatct atctctatgg
     5581 ctggaagcgg gtgccccctc gggcacacct gctggccggc gtggtggtgg cggtcagcgg
     5641 cgggctctcc gcggtcttcg tggtcaccgt caacgcctgg atgaacaccc ccaccgggtt
     5701 caagctggta gacggccggt tcgtcgacat cgatccggtg gcggcgatgc tcaacccggc
     5761 ggcgttcccg caggcgctcc acatgctgct ggccgcctat gccgcggtgg ggttcgcggt
     5821 ggccggcatc cacgccgccg tgctgctgcg ccaccccggc gaccgcttcc accgcgccgc
     5881 cctcgccatc gccctgctgg tcggcggacc ggcggcgctg ctccagccgc tctcggggga
     5941 catcctggcg aagatggtgg ccgacactca gccggccaag ctggcggcca tggaggggca
     6001 cttcgagact cagcaggggg cg
//
LOCUS       DASPJI010000032         3640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_516846, whole genome shotgun sequence.
ACCESSION   DASPJI010000032 DASPJI010000000
VERSION     DASPJI010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516846"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VEL74_00960"
     CDS             complement(<1..302)
                     /locus_tag="VEL74_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HYG61123.1"
                     /translation="MNPDVIEAIPRLVERGILPAERAPLLLRAARRELVSVRLELRLL
                     LYLGVLLVTGGVGLLIKENLDRIGPVVIASALGLAAAAALAWVWRTAPPFSWERVPS"
     gene            complement(339..1523)
                     /locus_tag="VEL74_00965"
     CDS             complement(339..1523)
                     /locus_tag="VEL74_00965"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HYG61124.1"
                     /translation="MKERKGRVLDLVSRIEGGFDRLKNRVAERFDRDDPLQILPYRGF
                     GTAERLIFQGRILQDEPVGTSSKGDTVWKNLGNMWKRFESDELPGVKVQARLGSWSWE
                     TVTDDEGYFRFDVRLPSPLTELAEPGRLWYEVALEMRDPRSGLPVRGTAPVLVPAPGA
                     ELGVISDIDDTVVETGVTNRLAMARTVFLHNAHTRLPFQGVAAFYKALHQGRRAREGR
                     GANPVFFVSGSPWNLYDLLAEFMDLHEIPRGPLMLRDFGFDRDKLLHTETREHKLACI
                     RPILELYPALSFVLIGDSGEQDPEIYREVVREFPGRVRAIYIRNVATRPARENEVLAI
                     AREVREEGVPMLLVKDTEAASVHALAEGWIRPEKLPEVRQEKLKDQAAPSPAEVALST
                     EA"
     gene            complement(1579..2385)
                     /locus_tag="VEL74_00970"
     CDS             complement(1579..2385)
                     /locus_tag="VEL74_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HYG61125.1"
                     /translation="MFLAAAVQLTSTSDEQANWESARALIERAAGHGASFVATPENTN
                     YLGPHEEKVRRAEPLDGSTVRRFAELARGLGIHLLLGSFNEKSDDAHRCYNTSVFFGP
                     DGSILGTYRKIHLFDVDVPGGVRFSESATCKPGEGTAVVDTPLGRFGLSICYDLRFAE
                     LYRQLADQGADILMIPSAFTLATGKDHWEPLIRARAIENQCWVIAPAQHGKHDDQGLS
                     HTWGHAMIVDPWGLPAATASDGPGIALAEIDLGRAAKVRQAIPVRQHRRL"
     gene            2489..>3640
                     /locus_tag="VEL74_00975"
     CDS             2489..>3640
                     /locus_tag="VEL74_00975"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ceramide glucosyltransferase"
                     /protein_id="HYG61126.1"
                     /translation="MTHTLLHTVALLAGVSLLLTLLATGAALRVLAHGRGRSGGDPQR
                     PLPSISILKPLKGVDEELYENLAALARQDYPVFELVLGAEDPGDPALAVAERLRRDFP
                     GVAITLVYGAGPLGYNPKVLNLASIARHARHEHLLISDSNVRPGPGYLRALASEMTEE
                     TGLVSSVLAGTGEASLGALFENLHLSSFVAASVCGAHLAGHPCVVGKSMLFRRAGLEA
                     LGGWGAVRDVLAEDYVLGQRFARAGWRVALSPHVLPVIHQRRPVAAFLERHLRWAQMR
                     RRLSPAFFGEPLLNPMPWLLGLLALSVTAGEAAWAGAALAGTGAKAAADARLAWRLRG
                     RALPVRSLAWIPAKDLLIAGVWLAGAFRSTICWRGHRLRVGPGSLLSPVE"
BASE COUNT          478 a         1293 c         1328 g          541 t
ORIGIN      
        1 gacggcaccc gctcccagga gaacggcggc gcggtgcgcc agacccaggc gagcgccgcc
       61 gcggcggcca gccccagcgc cgaggcgatg accaccgggc cgatgcggtc caggttctcc
      121 ttgatgagca ggcccacgcc gccggtgacc aggaggacac ccagatacag gaggagtcgc
      181 agctccaaac ggaccgacac cagctcccgg cgggcggccc gcaagaggag cggcgcccgc
      241 tcggcgggca ggatgccgcg ctccaccagc cgggggatgg cttcgatgac gtcggggttc
      301 acgatgggct ccgcggccca tcttatcgcg gatgcggtct aggcttcggt gctcagggcc
      361 acttcggcgg ggctgggcgc ggcctggtcc ttgagcttct cctggcgcac ctcggggagc
      421 ttctcgggcc ggatccagcc ctccgccagg gcgtggaccg aggccgcctc ggtgtccttg
      481 accagcagca tcggcacccc ctcctcccgc acctcgcggg cgatggccag tacctcgttt
      541 tcccgtgccg ggcgggtcgc cacgttgcgg atgtagatcg cccgcacgcg gccggggaac
      601 tcccgcacca cctcgcgata gatctccggg tcctgctcgc cgctgtcgcc gatcaggacg
      661 aaggagagcg cgggatagag ctcgaggatc gggcggatgc acgccagctt gtgctcgcgg
      721 gtctcggtgt gcaggagctt gtcgcggtcg aacccgaagt cgcgcagcat cagcgggccg
      781 cgggggatct cgtgcaggtc catgaactcg gccagcaggt cgtacaggtt ccacgggctg
      841 cccgagacga agaagaccgg gttggcgccg cgcccctccc gcgcccggcg cccctggtgg
      901 agcgccttgt agaaggcggc gaccccctgg aaggggaggc gggtgtgggc gttgtgcagg
      961 aagaccgtgc gcgccatcgc cagccggttg gtgacgccgg tctccaccac cgtgtcgtcg
     1021 atgtcgctga tgacgccgag ctcggcgccg ggcgccggca ccaggaccgg cgccgtcccg
     1081 cgcaccggca ggccggagcg cgggtcgcgc atctcgagcg ccacctcgta ccagagcctc
     1141 cccggctcgg ccagctcggt cagcggcgag ggcaggcgga cgtcgaaccg gaaatagccc
     1201 tcgtcgtcgg tcaccgtctc ccagctccag ctccccaggc gggcctgcac cttgacgccc
     1261 ggcagctcgt cgctttcgaa gcgcttccac atgttgccca ggttcttcca gaccgtgtcc
     1321 cccttgctgg aggtgccgac gggctcgtcc tggaggatcc ggccctggaa gatcagccgc
     1381 tccgccgtgc cgaagccccg gtagggcagg atctgcaacg ggtcgtcgcg gtcgaaacgc
     1441 tccgccaccc ggttcttgag ccggtcgaac cctccctcga tccgggagac gagatccagg
     1501 accctgcctt tgcgctcttt catagccgat cgcgcatatg caagaagcca gccagaaaag
     1561 gggaccggtc cgcggcggtt agaggcgccg gtgctggcgg accgggatgg cctggcgcac
     1621 cttcgcggcg cggccgagat cgatttctgc cagagcgatg ccagggccgt cggaggcggt
     1681 ggcggccggc agcccccagg ggtcgacgat catggcgtgc ccccaggtgt ggctgagccc
     1741 ctggtcgtcg tgcttgccgt gctgcgccgg cgcgatgacc cagcactggt tctcgatggc
     1801 gcgggcgcgg atcagcggct cccagtggtc cttgccggtg gccagggtga acgccgaagg
     1861 gatcatcagg atgtcggccc cctggtcggc gagttgccgg tagagctcgg cgaagcgcag
     1921 gtcgtagcag atggagaggc cgaagcgccc gagcggggtg tcgaccacgg ccgtcccctc
     1981 gccgggcttg caggtggccg actcggagaa gcggacgccg cccggaacgt cgacgtcgaa
     2041 caggtggatc ttgcggtagg tgccgaggat ggacccgtcg ggaccgaaga agacgctggt
     2101 gttgtagcag cggtgggcgt cgtcgctctt ctcgttgaag gagcccagga gcaggtggat
     2161 gccgaggccg cgggcgagct cggcgaagcg gcgcaccgtg gagccgtcga ggggctcggc
     2221 gcggcgcacc ttctcctcgt ggggtcccag gtagttggtg ttctcgggcg tggcgacgaa
     2281 cgaggcgcca tggccggcag cgcgctcgat cagggcgcgg gcgctctccc agttggcctg
     2341 ttcgtccgag gtggacgtga gctggacggc ggcggcgagg aacatgggcg ctttctagca
     2401 catcctgacc ctcctgtgac cccggcgcca cgcctccgtc acgccccggg tccatcctgg
     2461 cagcggaagg aaaggcagac cacccgagat gacccacacc cttctccaca ccgtcgcgct
     2521 gctggccggg gtgtccctgc tcctgaccct cctcgccacc ggtgcggcgc tgcgggtgct
     2581 ggcccacgga cggggccggt ccggagggga tccccagcgg ccgttgccgt cgatttcgat
     2641 cctgaagccg ctcaaggggg tggacgagga gctgtacgag aacctcgccg ccctcgcccg
     2701 ccaggactac ccggttttcg agctggtact cggcgccgag gaccccggcg accccgccct
     2761 cgccgtggcg gagcggctgc gccgcgactt ccccggcgtc gccatcaccc tcgtctacgg
     2821 cgccggtccc ctgggctaca accccaaggt gctgaacctg gcctccatcg cccggcacgc
     2881 ccgccacgag cacctgctga tctcggactc caacgtccgc cccgggccgg gctacctgcg
     2941 cgcgctcgcc tcggagatga cggaggagac cgggctggtc tccagcgtcc tcgccggcac
     3001 cggcgaggcg agcctgggag cactgttcga gaacctgcac ctgagctcgt tcgtcgccgc
     3061 ctcggtctgc ggcgcccacc tggcgggaca cccgtgcgtg gtgggcaagt cgatgctctt
     3121 ccggcgcgcc ggcctcgagg ccctgggggg ctggggcgcg gtgcgcgacg tcctggccga
     3181 ggactacgtg ctggggcagc ggttcgcccg cgccggctgg cgggtggcgc tctcgccgca
     3241 cgtcctgccg gtgatccacc agcggcggcc ggtcgcggcc ttcctggagc gccacctgcg
     3301 ctgggcccag atgcggcggc gcctctcccc cgccttcttc ggcgagccgc tgctgaaccc
     3361 gatgccctgg ctgctcggcc ttctcgctct ctccgtgacc gccggcgagg cggcgtgggc
     3421 cggagcggcc ctggccggaa ccggcgccaa ggcggcggcg gacgcccggc tcgcctggcg
     3481 gctgcgcggc cgggcgctgc cggtccgctc cctcgcgtgg attccggcca aggacctgct
     3541 gatcgccggc gtctggctgg cgggcgcgtt ccggtcgacc atctgctggc gcggccaccg
     3601 gctgcgggtg gggccgggga gcctgctctc gccggtggag
//
LOCUS       DASPJI010000033         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_573838, whole genome shotgun sequence.
ACCESSION   DASPJI010000033 DASPJI010000000
VERSION     DASPJI010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573838"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VEL74_00980"
     CDS             <1..698
                     /locus_tag="VEL74_00980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HYG61127.1"
                     /translation="RFLDRGTLAKVGARWPEGGKPRALRQAWTVPLGTLALLGSWLAA
                     TAVVAEVRLGGLSDVFEKGTSWWPGPLGSTAALVLYLMAFMVQGGLEEWVVRGYIYHA
                     LKERWRWWAAALSSSILFSALHASNPNITWVALVNIVLAGFILSELVERSGSLWSAVV
                     AHGVWNFVVACVLSLPVSGIRIFRLLDLGITGPPLLTGGEFGPEGSLILTFLGLVLAG
                     LLWPRRPGVDTPG"
     gene            complement(1034..2023)
                     /locus_tag="VEL74_00985"
     CDS             complement(1034..2023)
                     /locus_tag="VEL74_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009673232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HYG61128.1"
                     /translation="MLAVLPSEDHRPIPRVGEVPDPVPGPGEILVEVEAAGVNHAELL
                     QVRGLYPPPPGESDVPGLECAGRVAGDPRTEVPGTWQPGARVMALLAGGGHATRVAVP
                     AGQLMPLPGNLSYVEGGAIPEAALTAWTNLVAEGGLQPGETVLVTGASGGVGSFAVQL
                     ARELGARVLAAGRNRDRLEMLRELGIDEICLEGASLPQQVREATGGRGADLVLDLVSG
                     PHFPEHLAALREKGRLVLVGAVAGAKSEVELSSLLRRRLRVTGSVLRPRSREEKSRLV
                     SDFISFALPRLKDGRLRPVIDRVVPFDRAPEAYKALAQGGVFGKVVLDMSGSR"
     gene            complement(2043..>2344)
                     /locus_tag="VEL74_00990"
     CDS             complement(2043..>2344)
                     /locus_tag="VEL74_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HYG61129.1"
                     /translation="RAQAMREAGLDEADKAAELADLFCRNARRKVARLFRALWANDDA
                     RKYKVAMKVLDGQHSWLEAGILDLGARAEAMRPDNVFGGKAEPEPMPRVRPVGTN"
BASE COUNT          300 a          815 c          887 g          342 t
ORIGIN      
        1 tccgcttcct ggaccgcggc accctggcga aggtgggggc ccgctggccg gaggggggaa
       61 agccgcgggc gcttcggcag gcgtggacgg tgccgctggg gactctcgcg ctcctgggaa
      121 gctggctcgc ggccacggcc gtggtggccg aggtgcggct gggagggttg agcgacgttt
      181 tcgagaaagg gacctcctgg tggcccggtc ccctgggcag caccgccgcc ctcgtcctct
      241 acctgatggc cttcatggtc cagggggggc tcgaggagtg ggtggtgcgc ggctacatct
      301 accatgccct caaggagcgc tggcgctggt gggcggcggc cctctcctcc tccatcctct
      361 tctcggcgct tcacgcctcc aaccccaaca tcacgtgggt cgcgctggtc aacatcgtcc
      421 tcgcggggtt catcctgtcc gagttggtgg agcggagcgg cagcctctgg tcggcggtgg
      481 tggcgcacgg ggtgtggaac ttcgtggtgg cctgcgtgct gtcgctgccg gtgagcggca
      541 tccgtatatt ccgcctgctg gacctcggca tcaccggccc gccgctgctg accggcggcg
      601 agttcggccc ggaggggagt ctgatcctga cttttctggg gctcgtgctg gccggtctgc
      661 tctggccgcg gcgccccggg gttgacaccc caggctgaaa cgagacgatt cgctccgcgg
      721 ggcagacggg ttgacccgaa ggcaagggct cttgccccaa ggggtgaacg gctcgacccc
      781 agggaaaggg tgcttgcccc acggggcgaa cggcttgacg gtaagggggc ttgacccatg
      841 gggcaaacag cttgacccga gggtaaggag gcttgaccca cggggcagac ggctcgaccc
      901 gaggggcgcg agtgaaaaac ccccctcctg acctccccca ctccctacca cccacccgcc
      961 caggggggga ggcaccaccg ccccctgttc cggcggtccg tgctagtccg tgtcagtccg
     1021 tgttgttgtt cgcctagcgg ctgccgctca tgtcgagcac gaccttgccg aacactcccc
     1081 cctgcgccag ggccttgtag gcctcggggg cgcggtcgaa ggggacgacg cggtcgatga
     1141 ccgggcgcag ccggccgtcc ttgaggcgcg gcagggcgaa ggagatgaag tcggacacca
     1201 ggcgggactt ctcctcccgg gagcgggggc gcagcacgga gccggtgacg cgcaggcggc
     1261 ggcggagcag ggaggagagc tcgacctcgg acttggcgcc ggccaccgcg cccaccagca
     1321 ccagcctccc cttctcgcgc agcgccgcga ggtgctccgg aaagtgaggg ccactcacca
     1381 ggtcgagcac caggtcggcc ccccggccgc cggtcgcttc ccggacctgc tgcggcaggc
     1441 tggccccctc caggcagatc tcgtcgatgc ccagctcgcg cagcatctcg agacggtcgc
     1501 ggttgcggcc ggcggcgagc acgcgggcac cgagctcgcg ggcgagttgg acggcgaagc
     1561 tgccgacgcc gccggaggcg ccggtcacca gcacggtctc gccgggctgg aggcccccct
     1621 cggccaccag gttggtccag gccgtgagcg ccgcctcggg gatcgcgccg ccttctacat
     1681 aggagagatt gccggggagc ggcatgagct ggcccgcggg gacggcgacg cgggtggcgt
     1741 ggccgcctcc ggccaggagg gccatgacgc gggcgccggg ctgccaggtg ccggggacct
     1801 cggtgcgcgg atcgccggcg acgcgcccgg cgcactcgag gccggggacg tcgctctctc
     1861 caggcggcgg ggggtagagc ccgcgcacct ggagcagctc ggcgtgattg acgccggccg
     1921 cctcgacctc gacgaggatc tcgccggggc cggggaccgg gtcggggact tccccgaccc
     1981 gggggatcgg ccggtggtct tcggagggga ggacggcgag cacgggtgcc cgatccgccg
     2041 tcctagttgg tgccgaccgg gcggacgcgc ggcatcggtt ccggctccgc cttgccgccg
     2101 aagacgttgt ccggccgcat cgcctcggcg cgggcgccga ggtccaggat gcccgcctcg
     2161 agccaggagt gctggccgtc gaggaccttc atggccacct tgtacttgcg cgcgtcgtcg
     2221 ttggcccaga gcgcccggaa caggcgcgcc accttgcggc gggcgttgcg gcagaacagg
     2281 tcggccagct ccgcggcctt gtccgcctcg tccaggccgg cctcgcgcat cgcctgcgcc
     2341 cgcg
//
LOCUS       DASPJI010000034         2722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_232084, whole genome shotgun sequence.
ACCESSION   DASPJI010000034 DASPJI010000000
VERSION     DASPJI010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232084"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VEL74_00995"
     CDS             complement(<1..447)
                     /locus_tag="VEL74_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYG61130.1"
                     /translation="MRDERAGSGRRRVVVSGLGAVSGLGWGAGPLWEGLRAGRTGIRP
                     FERFDHSGHRTHLAAEVPPRPPGLAVAVLERRFPVWRRLSLADRYALFATAEALAQAG
                     LEGLPGDSAETGVFFGSSTGGMFESEHFFADFLRDRRKARLSALVAQ"
     gene            complement(484..744)
                     /locus_tag="VEL74_01000"
     CDS             complement(484..744)
                     /locus_tag="VEL74_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYG61131.1"
                     /translation="MSNAVATPHRIKRLIVESLHLEGVKPEMIEDEAPLFGEGLGLDS
                     VDALELVVALEREFGIKIKSQELGREVFSSVSSLSEFIEART"
     gene            1039..2472
                     /locus_tag="VEL74_01005"
     CDS             1039..2472
                     /locus_tag="VEL74_01005"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HYG61132.1"
                     /translation="MTRDPIARAFDRLVQRDPAAPLVLTPERRATVGDVEALAQAAGS
                     VTAAAGLAPGTLIGLVAPNGPGMLASLVALARAGIAVLLLDGQTPEAEGMRIAHALGA
                     AALLRCHTGWPAGPADWSLAPAASEPRWLPDTAVVKLTSGSTGAPRGIATSAEALLAD
                     DEALTATMGIGPDDRLLATVPFSHSYGLSSLVIPCLVRGTVLVMPEESSLFDPFFAGR
                     QAGATVFPSVPAYLDALLRLSEPTPPPPGLRLVLTAGAPLRPETSLRFRETFGLPVHV
                     FYGASECGGICYDREGGAAERGTVGAPVEGVRVTLAPEEHDEAEGGHPGEGIVTVESA
                     AVARGYLPDPEPRLQDGRFVAGDRAVWRDGELALLGRRDDQVNIKGKKVNPREVEGVL
                     AQLQGVDEVAVLGVPAPRHPQQGELLRAVVACRPGRLTAEDVLGWCRSRLAPHKVPRS
                     IILVPELPRTSRGKLDRPALRALGGGG"
     gene            2469..>2722
                     /locus_tag="VEL74_01010"
     CDS             2469..>2722
                     /locus_tag="VEL74_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYG61133.1"
                     /translation="MKPRWRPTPLMAASIGIHAAAAVSLAVAPRHWPWIAGGLFANHV
                     VLAAASLTPRSRLLGTNLSRLPEAAARRGEVALTFDDGPDP"
BASE COUNT          345 a         1028 c          989 g          360 t
ORIGIN      
        1 ctgcgccacc agggcggaca gccgggcctt gcggcggtct ctgaggaaat cggcgaagaa
       61 gtgctcgctc tcgaacatgc cgccggtgct gctgccgaag aagacgccgg tctcggccga
      121 gtcccccggc agaccctcca accccgcctg ggcgagggcc tcggcggtgg cgaagagggc
      181 gtagcggtcg gcgaggctga ggcggcgcca gacggggaac cgccgctcca gaaccgcgac
      241 ggccagaccg gggggccgcg gtgggacttc ggcggcgagg tgggtgcggt ggccggagtg
      301 gtcgaagcgc tcgaaggggc ggatgccggt gcggccggcg cgcagcccct cccacagcgg
      361 cccggcgccc cagccgagac cgctcacggc tccgaggccg ctcaccacga cacggcgccg
      421 gcccgaaccg gctctctcgt ctctcatgtg gggacccgcg aggcgacccg ccaagcgcgg
      481 ggcctacgtg cgggcctcga tgaactcgga caggctggag accgaggaga agacctcgcg
      541 tcccagctcc tggctcttga tcttgatgcc gaactccctt tccagggcga cgaccagctc
      601 gagagcgtcg accgaatcga gccccaggcc ctcgccgaac agcggggcct cgtcctcgat
      661 catttcgggc ttgaccccct cgaggtggag gctctcgacg atcagacgct tgatcctgtg
      721 aggagtcgcg acggcgtttg acatagttga aacctcagtg ctccgactca cctgacctcc
      781 ggccggagca agccccttca gccggctccg cagcccccga cactccctcg ccccccgcta
      841 cggcccggcc gtgcggcagc ttcgaggctg gccatgcacc gggtgcgcca atgctagcgt
      901 gcgcgcgggg gccctggcaa tgggccacgg gcgcagtatc attcatcgcc gcccgcgctg
      961 tcctcgaaag gcgggacggg cggcaccggc acccgatctc ttgaacagcg gcgctccccc
     1021 ggccatttca gaaacggtat gacccgagac cccatcgccc gagcgttcga ccggctggtc
     1081 cagcgcgatc cggcggcgcc gctggtgctg acgccggagc gccgggccac ggttggggac
     1141 gtggaggccc tggcccaggc ggccgggtcg gtgaccgccg ccgccgggct ggcgccgggg
     1201 acgctgatcg gcttggtggc ccccaacggc ccggggatgc tcgcctcgct cgtcgccctg
     1261 gcccgggccg ggatcgccgt cctgctgctg gacggccaga ccccggaggc ggaggggatg
     1321 cggatcgccc acgcgctcgg agccgcggcc ctgttgaggt gccacaccgg ctggccggcc
     1381 ggcccggccg actggtccct ggctccggcc gccagcgagc cccgctggct gccggacacc
     1441 gcggtggtga agctgacctc cggctccacg ggggcgccac gggggatcgc cacctcggcc
     1501 gaggccctgc tcgccgacga cgaggcgctg accgccacca tgggaatcgg gccggatgac
     1561 cgcctgctcg ccacggtccc cttctcccat tcttatggac tgtcgagcct ggtgatcccc
     1621 tgcctggtcc gcggcacggt gctggtgatg cccgaggaga gcagcctgtt cgaccccttc
     1681 ttcgccggcc ggcaggcggg ggccacggtc ttcccctcgg tgccggccta cctggacgcc
     1741 ctgctgcggc tgtcggagcc gactcctccc ccaccgggcc tgcggctggt gctgaccgcc
     1801 ggcgcgccgc tgcggcccga gacctccctg cgcttccggg agaccttcgg cctgccggtg
     1861 cacgtcttct atggcgccag cgagtgcggc ggcatctgct acgaccgcga ggggggcgcc
     1921 gccgagcggg ggacggtggg cgcgcccgtc gaaggtgtgc gggtgacgct ggcccccgaa
     1981 gagcatgacg aggccgaagg cggccacccc ggcgagggga tcgtgaccgt cgagtccgcc
     2041 gccgtcgccc gcggctacct ccccgatccc gagccccggc tccaggacgg ccgcttcgtc
     2101 gccggcgacc gggcggtctg gcgggacggc gagctggccc tcctcggccg gcgggacgac
     2161 caggtcaaca tcaaggggaa gaaggtgaac ccgcgcgagg tcgaaggggt gctggcgcag
     2221 ctccaggggg tggacgaggt ggcggtgctc ggggtgccgg cgcctcgcca tccgcagcag
     2281 ggcgagctgc tgcgcgccgt cgtggcctgc cggccggggc gcctgaccgc cgaagacgtg
     2341 ctcggctggt gccgctcccg tctcgccccc cacaaggtgc cgcgcagcat catcctggtc
     2401 ccggagctcc cccgcacctc ccggggcaag ctggaccggc cggcgttgcg cgccctggga
     2461 ggagggggat gaagccccgc tggcgtccca ccccgctcat ggccgcatcc atcggcatcc
     2521 acgccgccgc cgcggtgtcc ctcgccgtgg cgccgcggca ctggccctgg atcgccggcg
     2581 gcctgttcgc caaccacgtg gtcctcgccg cggcgagcct gacgccccgc tcgcggctcc
     2641 tgggcaccaa cctctcccgc ctgcccgagg ccgctgcgcg ccggggggag gtggcgctga
     2701 ccttcgacga cggccccgac cc
//
LOCUS       DASPJI010000035         6310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_573973, whole genome shotgun sequence.
ACCESSION   DASPJI010000035 DASPJI010000000
VERSION     DASPJI010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573973"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..>6310
                     /locus_tag="VEL74_01015"
     CDS             101..>6310
                     /locus_tag="VEL74_01015"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61134.1"
                     /translation="MEKTVSQGFRLSPRQQHVWRAQRGESSPGLVVQTRVDVSGPLDR
                     RRLARAVEWTVERHEILRTTFPLLPSLSIPVQSVEDAAAVDWSEHSLAGRSGAEREAA
                     LDRLAAEMRESPFDLTRGPLLRVTLAGLEGEPGRELHALLLTQPALCADGASLDLMAE
                     EIIAAYRGEPAGDDALQYADLAEILQDWEASPADDPGAAFWRAQDLSALGSALGRGEP
                     DTGRPFRPETVSRSLDRDRLEEAAARLGAPVSPLLLAAWQGTLCLSLGEEEIVVGTLF
                     EGRTLEELESALGPFARYLPVHGLSTADSTLEELSRTLGATVNEMARRQDLFSSESLA
                     QRLAGHGLPGWPFGFDFRRPRELPAGGLPGFTIRGGYLGLDRFDARLSVLDDGRSLRA
                     GLVHDPVRLGRGEAERLLERFTRVLDGLLAGYPLGEIDVLTESERRQVLDLNRIESPV
                     PADLCAHHLFEEQVRRVPGATALRHLGETWTYAELNARANRLAHHLRASGLGPGSRAG
                     IFLERSPELVAAVLGVLKAGAAYVPLDPGSPAGRRAAMAEDAGLSVLITREWLDGHRE
                     EISLRSEEDPPRVAGPSDLAYILYTSGSTGRPKGVMVPHRGLVNYLLWAREAYAAGEG
                     AGAPVHSAIGFDLTVTSLLVPLAAGTAVLLLEEGGSPVEALAGALRGTAGFSLVKLTP
                     AHLDLLGRELRPEEHAGRTRALVIGGEALHVDRVAPWRQHAPDTRLINEYGPTEATVG
                     CCVYEIGPEDAAAGPVPIGRPIANTRLYVVGPRLRPVPCGAPGELWIGGDGLARGYLN
                     RPDLTAERFVPDPFADEGRAGGRLYRTGDLVRLGMEEQLEFLGRVDDQIKIRGFRIEP
                     GEIEAALTAHDGVREAAVLAAEEPAGVPILVACVAGKAGAGALRRHLLERLPEPMVPS
                     RFAFLDTLPLTPNGKVDRQALARTWRERATSREESVADEAPRTWTEELLAGMFADLLG
                     TPSVGRNESFFALGGHSLLATQAISRVREAFGVEISLRGFFEVPTVASLAAKVEALRA
                     RGETVDLPPLLPRKSIPRDTPPPLSFAQQRLWFIAQLDPASPLYNIPVALRVEGALRP
                     EVLALCLAEIARRHEALRTVFATRDGEPVQVVRPAADPGLGMVDLAGLPEHRREAAAL
                     ALAAGEAARPFDLARGPLFRALLLRLGVEDHVAVLTLHHIVSDGWSMGVLVREVGALY
                     ATFAEGRPLSRAPLPELPVQYADFAVWQRSWLRGEALERQIAFWRRQLAGLPPLLELP
                     TDRPRPAALSYRGATRPVRLPAALIRRLQALGRREGATLFMVLLAGFQALLARHSGQQ
                     DLAVGTPVAGRNRIETEGLIGFFVNTLVLRGDLTGDPPLRELVGRARDAALAAWAHQD
                     VPFEKLIEELSPERSLAHTPLFQTVLALHNAPVGSLEVQDLRLRPLSGAGTTAKFDLL
                     FSFEEWDGGLIGTVEHSTDLFDAATIERLFLQYERLLAAAVEEPDLAVPDLPLLPDSE
                     RHQILAEWNDTGAGRGESTLIHELFEDWAARTPGAIAVVCGGESLTYGDLAERAHHLS
                     WHLIGLGVGPGSLVGIHLRRSLDMIPALLAVLEAGAAYVPLESGHPPARLQWIVGALD
                     ISCVLTESAQRGSLPELPHVICLDRVELERERPVAPPRRGAADDLAYIIFTSGSTGTP
                     KGVMVRHRPVVNLFRWAYRTLEFSPADRVLFITSLAFDLSVFDVFGLLGAGGSIRVAT
                     EEEIRDPQLLLRALAEEPITFWDSAPAALEQTVPFLAGMDPRNRPALRLVFLSGDWIP
                     VTLPDRVRERFPEARVISLGGATEATVWSNVFPIGKVDPAWTSIPYGRPIGNARYHVL
                     DARLSPCPVGVPGDLYIGGDCLADGYAREPELTAGKFLPDPWSATPGARLYRTGDRAR
                     YRPNGNLEFLGRLDHQVKIRGFRIELGEVEAALATLPGVRDAVVVVREDTPGDRRLVA
                     YVTGDAAAADELRLSLQERLPEPMVPAAFVSLAALPLTPNGKVDRKALPAPEWQRAEE
                     SHLAPRTPAEEIVAGIWAEVLGLDRVGATDHFFELGGHSLLATQVISRLGEAFG"
BASE COUNT          753 a         2364 c         2282 g          911 t
ORIGIN      
        1 gagaagcgga gggtctcgct ggcccgggcg cgccgccgcg tcctggtgga ccgggaggcc
       61 tcttgaccgg gacgccgagc cgcggacaac ggagatcctc atggagaaga ctgtcagcca
      121 gggatttcgc ctctcgcccc ggcagcagca cgtctggcgg gcgcagcgcg gcgagagctc
      181 tcccgggctc gtggtccaga cccgcgtcga cgtctccggc cccctcgacc gccggcgcct
      241 ggcacgggcc gtggaatgga cggtggagcg tcacgagatc ctgcgcacca ccttcccgct
      301 gcttccctcc ctgagcattc cggtccagtc ggtggaggac gccgccgcag tcgactggag
      361 cgagcacagc ctggccggcc gtagcggcgc ggagcgcgag gccgccctcg accggctcgc
      421 tgccgagatg cgggagagcc cgttcgacct cactcggggt cccctgctgc gggtcacgct
      481 ggccgggctg gagggcgagc ccggccggga gctccacgcc ctgctcctga cccagcccgc
      541 gctctgcgcc gatggcgcct ccctcgacct catggccgag gagatcatcg cggcctaccg
      601 cggcgagccc gccggggacg acgcgctcca gtacgccgac ctcgccgaga tcctccaaga
      661 ctgggaggcc tctccggccg acgatcccgg cgccgccttc tggcgcgcgc aggatctgtc
      721 ggcccttggg agcgccctgg gccggggcga gccggacacc ggccggccct tccgtccgga
      781 gaccgtgagc cgaagcctgg accgggaccg gttggaggag gccgcggcgc ggctcggcgc
      841 cccggtctct cctctcctcc ttgccgcctg gcaggggacg ctgtgcctct ccctgggcga
      901 ggaggagatc gtcgtcggca cgctgttcga gggcaggacc ctcgaggagc tggagagcgc
      961 cctcgggccg ttcgcgcgct acctcccggt ccacgggctc tccacggcgg attcgaccct
     1021 ggaggagctg agccggaccc tgggcgccac cgtgaacgag atggcgcggc gccaggacct
     1081 cttctcgtcc gagtcgctgg cgcagcggct cgccgggcac ggccttccgg gttggccttt
     1141 cggattcgat tttcggcgcc cgcgggagct gccggccggg gggctcccgg ggttcacgat
     1201 ccgcggcggt tacctggggc tcgaccggtt cgacgcgcgc ctgtcggtcc tcgacgacgg
     1261 ccggtccctg cgcgccggcc tggtccacga cccggtccgt ctcgggcgcg gcgaggccga
     1321 gcgcctgctg gagcggttca cccgcgtgct ggacgggctg ctcgccggct accccctggg
     1381 ggagatcgac gtcctcacgg agtccgagcg gcggcaggtg ctcgacctca accgcatcga
     1441 gagcccggtc cccgcggacc tctgcgccca tcatctcttc gaggagcagg tccgccgcgt
     1501 gccgggggcc acggccctgc gccacttggg cgagacctgg acctacgccg agctgaacgc
     1561 ccgtgccaac cggctggccc accacctgcg cgccagcgga ctgggcccgg gctcccgcgc
     1621 cggtatcttc ctggagaggt cgcccgagct ggtcgcggcc gtgctcggcg tcctcaaggc
     1681 gggcgccgcc tacgtgccgc tcgatccggg atcgccggcc ggacgccggg ccgccatggc
     1741 cgaggacgcc gggctctccg tgctgatcac ccgcgagtgg ctcgacggcc accgcgagga
     1801 gatctccctc cggagcgagg aggacccgcc gcgcgtcgcc ggcccgtccg acctggcgta
     1861 catcctctat acctccggct ccaccggccg gcccaaaggg gtgatggtcc cgcaccgcgg
     1921 cctggtcaac tatctgctct gggcgcgtga ggcctacgcg gccggcgagg gggcgggggc
     1981 gccggtccac tcggccatcg gtttcgatct caccgtcacc agcctcctcg tgccgctcgc
     2041 cgcgggaacc gccgtcctgc tcctggagga ggggggcagc ccggtggagg ccctcgccgg
     2101 cgcgctacgc ggcacggccg gcttcagcct ggtcaagctc accccggcgc acctcgacct
     2161 gctgggccgc gagctgcgtc ccgaggagca cgcggggcgc acccgggcct tggtcatcgg
     2221 cggcgaggcg ctccacgttg accgcgtcgc gccctggcgg cagcacgctc cggacacgcg
     2281 gctgatcaac gagtacggtc cgacggaggc gacggtcggc tgctgcgtct acgagatcgg
     2341 gccggaggac gccgcggccg gccccgtgcc catcggccgc cccatcgcca acacccgcct
     2401 ctacgtggtc gggccccgtc tgcggcccgt gccctgcggc gcgccgggcg agctgtggat
     2461 cggcggcgac gggctcgcgc gcggctacct gaaccgcccc gacctgaccg ccgagcgctt
     2521 cgtccccgac ccgttcgccg acgaggggag ggcgggcggg cgcctctacc ggacgggaga
     2581 cctggtccgg ctgggaatgg aggagcagct cgagttcctc ggccgcgtcg acgaccagat
     2641 caagatccgc ggcttccgta tcgagccggg cgagatcgaa gccgcgctca ccgcccatga
     2701 cggcgtgcgg gaggcggcgg tgctggcggc cgaggagccg gccggcgtcc ccatcctggt
     2761 ggcctgcgtg gccgggaagg ccggtgccgg cgcgctacgc cgtcacctcc tggagcggct
     2821 gccggagccc atggtcccgt cccgcttcgc cttcctggac accctgccgc tgacgcccaa
     2881 cggcaaggtg gaccgccagg cgctggcccg cacctggcgc gagcgggcca cctcccggga
     2941 ggagtcggtc gcggacgaag cgccgcggac ctggaccgaa gagctcctgg ccgggatgtt
     3001 cgccgacctg ctgggaacgc catccgtcgg ccgcaacgag agcttcttcg ccctcggcgg
     3061 ccactccctg ctcgccaccc aggcgatctc ccgggtacgg gaggcgttcg gcgtggagat
     3121 ctccctgcgc ggcttcttcg aggtgcccac cgtggcatcc ctggccgcga aggtggaggc
     3181 gctccgcgcg cgcggcgaga cggtggacct gccacctctt ctaccccgaa agtcgatacc
     3241 gcgggacacg ccgccccctc tgtcgttcgc ccagcagcgc ctctggttca tcgcccagct
     3301 cgatccggcg agcccgctct acaacatccc ggtggctctg cgggtcgaag gggcgttgcg
     3361 gcccgaggtg ctggcgctct gcctcgcgga gatcgcgcgc cggcacgagg cgctgcgcac
     3421 ggtgttcgcc acgcgggacg gcgagccggt ccaggtggtc cggccggcgg cggaccccgg
     3481 gctgggtatg gtcgatctgg cgggtctgcc ggagcaccgc cgggaggcgg cggccctcgc
     3541 ccttgccgca ggggaggccg cccggccgtt cgacctggcg cgcggcccgc tgttccgggc
     3601 tctgctgctg cggctggggg tggaggacca cgtcgccgtc ctcaccctgc atcacatcgt
     3661 cagcgacggc tggtccatgg gagtcctcgt ccgcgaggtc ggcgccctgt acgccacctt
     3721 cgccgagggc cggccgctgt cccgggcgcc gttgcccgag ctgccggtgc agtacgcgga
     3781 cttcgcggtc tggcagcgct cctggctgcg cggcgaggcc ctcgagcgcc agatcgcctt
     3841 ctggcggcgg cagctcgcgg gcctgccccc actcctcgag ctccccaccg accgtccccg
     3901 gccggcggcg ctgagctacc gcggcgccac gcggcccgtg cggctgccgg cggcgctcat
     3961 ccggcggctg caggccctcg gccggcgcga gggggcgacc ctgttcatgg tgctgctggc
     4021 cggattccag gccctgctcg cgcgccacag cggacagcaa gacctcgccg tgggcacgcc
     4081 ggtcgccggc cgcaaccgca tcgagaccga aggcctgatc gggttcttcg tcaacacgct
     4141 ggtgctgcgc ggcgacctta ccggcgatcc gccgctgcgc gagctggtcg gccgggcacg
     4201 cgacgccgcg ctggccgcct gggcgcacca ggacgtgccg ttcgagaagc tgatcgagga
     4261 gctgtcgccg gagaggagcc tcgcccacac ccccctgttc cagacggtgc tcgcgctgca
     4321 taacgccccg gtcggcagcc tggaggtcca ggacctgcgc ctgcggccgt tgagcggcgc
     4381 cgggaccacg gcgaagttcg atctcctgtt cagtttcgag gagtgggacg gcgggctgat
     4441 cggcaccgtg gagcactcca ccgacctgtt cgacgccgcg accatcgagc gcctgttcct
     4501 ccagtacgag cggctgctcg cggcggcggt ggaggagccg gacctcgcgg tgccggacct
     4561 gccgctgctg ccggactccg agcgtcatca gatcctggcg gagtggaacg acaccggggc
     4621 cggccggggt gaatccaccc tgatccacga gctgttcgaa gactgggcgg cgcggacccc
     4681 cggcgccatc gccgtggtct gcggcggcga gagcctcacc tacggcgacc tggccgagcg
     4741 agcacaccat ctgtcctggc acctgatcgg cctcggcgtc ggcccgggct cgctggtggg
     4801 catccacctg cgccgctcgc tggacatgat tccggccctt ctcgccgtgc tcgaggcggg
     4861 cgccgcctac gtgccgctgg agagcggcca tccgccggcc cgcctgcaat ggatcgtcgg
     4921 cgcgctggac atctcctgcg tgctcaccga gtcggcgcag cggggctccc tgccggagct
     4981 gccgcacgtg atctgtctgg atcgcgtcga gctggagcgg gagcggccgg tggcgccgcc
     5041 gcgccggggg gctgcggacg acctggccta catcatcttc acctcaggct ccacgggcac
     5101 acccaagggg gtgatggtac gccaccgccc ggtggtcaat ctgtttcgct gggcttaccg
     5161 caccttggaa ttctccccgg ctgatcgggt gctcttcatt acctccctcg ccttcgacct
     5221 gtcggtcttc gacgtcttcg gcctgctggg ggcgggcggc tcgatccgcg tcgccacaga
     5281 ggaggagatc cgcgatcccc agctcctgct gcgcgccctg gccgaggagc cgatcacctt
     5341 ctgggactcg gcgccggctg cgttggagca gacggtgccg ttcctcgccg ggatggaccc
     5401 gcggaaccgc ccggccctgc gcttggtctt cctgagcggc gactggatcc cggtgaccct
     5461 gccggaccgc gtccgcgagc gcttccccga agcccgggtg atctccctgg gcggtgccac
     5521 cgaggccacg gtctggtcca acgtcttccc catcggcaag gtcgatccgg cctggacgag
     5581 catcccgtac ggccggccca tcggcaacgc gcgctaccac gtgctcgacg cgcggctctc
     5641 cccctgcccg gtgggtgtgc cgggagacct ctacatcggc ggggactgcc tggccgacgg
     5701 ctatgcccgg gagccggagc tgaccgccgg caagttcctc cccgacccgt ggagcgccac
     5761 gcccggcgcc cggctctatc gcacgggcga ccgcgcccgc taccgtccga acggcaacct
     5821 ggagttcctg gggcggctgg accaccaggt caagatccgc ggcttccgca tcgagctggg
     5881 ggaggtcgag gcggcgctcg ccacgctccc gggagtccgc gacgcggtcg tggtggtgcg
     5941 ggaggacacc cccggcgacc ggcggcttgt cgcctatgtg accggcgacg ccgccgcggc
     6001 ggacgagctg cgcctctcgc tgcaggagcg gctgccggag cccatggtcc cggccgcctt
     6061 cgtgtctctg gcggcgctcc ccctgacgcc gaacggcaag gtcgaccgga aggcgctgcc
     6121 cgctccggag tggcagcgcg ccgaggagag ccatctcgcg ccgcgcaccc cggcggagga
     6181 gatcgtggcg gggatctggg ctgaggtcct gggactcgac cgggtgggcg cgaccgatca
     6241 cttcttcgag ctgggtggcc actcgctcct ggcgacgcag gtgatctcgc gcctgggaga
     6301 ggcgttcggg
//
LOCUS       DASPJI010000036         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_803344, whole genome shotgun sequence.
ACCESSION   DASPJI010000036 DASPJI010000000
VERSION     DASPJI010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803344"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VEL74_01020"
     CDS             complement(<1..795)
                     /locus_tag="VEL74_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61135.1"
                     /translation="MNDERNRNSSTAGTGTLEAEHEHRDANRDPLSGESGAHPVGTGV
                     GAATGGTVGAVVGGAVGGPVGAMIGAAVGGLAGGLAGKGVAESVNPSEEDAFWRDNYS
                     NRNYAQGGRSYDEYRPAYQYGWESRARYADRSWNDVEHDLEHGWNQTKGTSNLGWSDA
                     RHATRDAWDRVDDRFRANRSGEGTASVSGSYGAASMSSGTENRGGTGTGDFTGAAGNL
                     SGNTGGMSGGGSGMSGGSSTGNLSSGTGTTGFASTTGANTGTANVGT"
     gene            936..1301
                     /locus_tag="VEL74_01025"
     CDS             936..1301
                     /locus_tag="VEL74_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61136.1"
                     /translation="MRGSPAWRTFWVPLLAIALLFAGLDLHPEGEPHGPLALTGSETY
                     FPEAAHPTLPDHVEAAHAAERPHCTACLQRLGSLGVQFEAAVSLAPPLPRVAMLPAGD
                     PSLLRRSLRPSGARAPPLS"
     gene            1379..>2782
                     /locus_tag="VEL74_01030"
     CDS             1379..>2782
                     /locus_tag="VEL74_01030"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYG61137.1"
                     /translation="MRDRMSWRALLWPLAVVLIIFLRAAPAAAQSTAAQPAPPAPVAP
                     RAAGAIAGTVTTLEGEPVTDAEVQLVELRRRAEVGADGSFRFDALPPGPYLLEATSPR
                     LGTAVQRVVVPSGGTAQAPLTLDLTVHQDEILVTASPDARSRNEVAQPTSVLSGEELQ
                     RQLQPTLGETLNEQPGVTSTSFGMGASRPVIRGLGGDRIRVLESGLGSGDASTTSPDH
                     AVSVDPLSAERIEVLRGPATLLYGSSAVGGVVNVIDNRIPDALPEEAVAGTVEVRGGS
                     VADERGGAAALQGGFGRFAWSASFHTRETDDYDIPGFAESEALRRAEEEEGEEHEQAT
                     GRLPNSATDSEGGSLGASWIGDGGFLGVSVTGFDSLYGIPGGHEHHHEEGEEHEEEEE
                     GEEPGVRVDLTQRRYDLRGGVTRPFAFFRGANVRFGVSDYEHQELEGEEVGTLFTSDS
                     REGRVELLQRQVGPLTGS"
BASE COUNT          363 a         1052 c          978 g          389 t
ORIGIN      
        1 ggtcccgacg ttggcggtgc cggtgttggc gccggtggtc gaggcgaagc cggtcgtgcc
       61 ggtgccgctg gacaggttgc ccgtggacga tccgccggac atcccgctgc cgccgccgga
      121 catcccgccg gtgttgcccg acaggttgcc ggccgcgccg gtgaaatcac ccgtgccggt
      181 gccgccgcgg ttctcggtgc ccgagctcat cgaggcggcg ccgtagctgc cggagacgga
      241 cgcggtgccc tcgccggagc ggttggcgcg gaagcgatcg tcgacgcggt cccaggcgtc
      301 gcgggtggcg tgccgggcgt cgctccagcc caggttcgag gtgcccttgg tctggttcca
      361 gccgtgctcg aggtcgtgct cgacgtcatt ccagctgcgg tccgcgtaac gagcgcggga
      421 ctcccagccg tactgataag ccggccggta ctcgtcatag gaacggccgc cctgggcata
      481 gttccggttg gagtagttgt cgcgccagaa ggcgtcctct tcactggggt tgaccgactc
      541 ggccacgccc ttgccggcga ggccgccggc caggccgccg acggcggcgc cgatcatggc
      601 gccgaccggg ccgcccaccg cgccgccgac cacggcaccc acggtgccgc cggtggccgc
      661 gcccacgccg gtgcccacgg gatgggcgcc ggactcacca ctcaggggat cacggttggc
      721 gtcgcgatgc tcgtgctcgg cctccagagt gcccgtacca gccgtggaag aattccggtt
      781 tctttcgtcg ttcataggtg ttcctcctgg aagtactacg ttgctcgtca gtagggtccg
      841 gcgccctccc ggataagacg ggattgcaaa gtcggggcca agcggcgtgc cgcttctggt
      901 accatccacc ggtcatggtg gcgaggggct catcgttgcg cggatctccg gcctggcgga
      961 ccttctgggt cccgctgctc gcgatcgcgc tgctgttcgc cggcctggat ctccatccgg
     1021 agggggagcc ccacggtccg ctcgctctga ccggcagcga gacctacttc cccgaggccg
     1081 cccacccgac gctgcccgac cacgtcgagg cggcgcacgc cgccgaacgg ccccactgca
     1141 cggcctgcct ccagcgtctc ggcagcctcg gcgtccagtt cgaggccgcc gtctccctgg
     1201 cgccgccgct gccgcgggtc gccatgctgc cggcgggcga cccctcgctg ctgcggcgct
     1261 ccctgcgtcc cagcggcgcc cgcgctccac ccctctcctg atcgttccac gtccagtctt
     1321 cacgacctga cctgaacgaa cgacaccggc gcgaggcgcc ggatcaggag attttggtat
     1381 gcgagaccgc atgtcctggc gggcgctgct ctggccgctc gccgttgtgc tcatcatctt
     1441 cctcagggcc gcgccggccg ccgcccagtc caccgccgcc cagccagcgc cgccggcgcc
     1501 agtggctccg cgggcggccg gcgccatcgc cggcaccgtc acgaccctgg aaggggagcc
     1561 cgtcaccgac gcggaggtcc agctcgtcga gctccgccgc cgcgccgagg tgggggccga
     1621 cggatcgttc cgcttcgacg ccctgccgcc cggcccctat ctgctggaag ccaccagccc
     1681 gcgcctcggc accgcggtcc agcgggtcgt ggtgccctcc ggcggcaccg cgcaggcccc
     1741 cctcacactc gacctcacgg tgcaccagga cgagatcctg gtcaccgcca gccccgacgc
     1801 ccgcagccgc aacgaggtgg cccagcccac ctccgtgctg tcgggcgagg agctgcagcg
     1861 gcagctccag ccgactctgg gggagacgct caacgagcag ccgggcgtca cctccacctc
     1921 gttcggcatg ggcgcgagcc ggccggtgat ccgcggcctc ggcggcgacc gcatccgcgt
     1981 cctcgagtcg ggcctgggca gcggcgacgc ctccaccacc agccccgacc acgcggtgag
     2041 cgtcgatccg ctctccgccg agcgcatcga ggtgctgcgc ggacccgcca ccctgctcta
     2101 cggcagcagc gccgtgggcg gcgtggtcaa cgtcatcgac aaccgcatcc ccgacgcgct
     2161 gccggaggag gccgtcgccg gcaccgtcga ggtgcgcggc ggcagcgtcg ccgacgagcg
     2221 cggcggcgcc gccgcgctcc aggggggctt cggccggttc gcctggagcg ccagcttcca
     2281 cacgcgcgag accgatgact acgacatccc cggcttcgcc gagtcggagg ccctgcgccg
     2341 cgccgaggaa gaggaagggg aggagcacga gcaggccacc ggccggctgc cgaacagcgc
     2401 caccgacagc gaagggggga gcctcggcgc ctcgtggatc ggcgacggcg gcttcctcgg
     2461 cgtctcggtg accggcttcg acagcctcta cggcatcccg ggcggacacg agcaccacca
     2521 cgaggagggg gaggagcacg aggaagaaga ggagggggag gagccgggcg tacgggtcga
     2581 cctgacccag cgccgctacg acctgcgcgg cggcgtcacc cgccccttcg ccttcttccg
     2641 gggtgccaac gtccgtttcg gcgtctccga ctacgagcac caggagctcg agggggagga
     2701 ggtcggcacc ctcttcacca gcgacagccg cgagggccgg gtggagctgc tccagcggca
     2761 ggtgggcccc ctgaccggct cg
//
LOCUS       DASPJI010000037         5957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_116801, whole genome shotgun sequence.
ACCESSION   DASPJI010000037 DASPJI010000000
VERSION     DASPJI010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5957)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5957
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116801"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..489
                     /locus_tag="VEL74_01035"
     CDS             190..489
                     /locus_tag="VEL74_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61138.1"
                     /translation="MRSDYQVFKLWFMAVLPLAKDAIHVYVGFGCLLVALLLLRLKLS
                     SFWCLVPGLVVSLIMEALDLRDGYAWGESLKDLINTNAIPFILVALARWRVFNIY"
     gene            515..1648
                     /locus_tag="VEL74_01040"
     CDS             515..1648
                     /locus_tag="VEL74_01040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG61139.1"
                     /translation="MEKVDHWQSLWGQTYADKPTEGDPTLVLSGWNSSYTGKPIRPDE
                     MKDWVERTVERILAPSPRRVLELGCGMGLLLFRVAPKVGAYWGVDFSAEALAHVGEHA
                     ARLGLANVELLQQGADDFTRLRQAEPFDTLVINSVLQYFPDMDYLLRVLESAQEILVP
                     GGRIFLGDIRSLPLLSAFHASVQMLQADGTTPLAELRERVEKLTREERELALAPSFFA
                     ELADLRRRFPRITRVEIQLKRGRYRNELTRFRYDAVLWLDGSGAVPDPESWLDWRGEG
                     LTLPDLKRRLAQEGPALLGVRGIPNARILPETRAVELIQAGASEAGPLHTVDDLLAAM
                     RGVRGGIEPEDLWALSKTLPYDIEVGWGEDHPDGAFQAVFRRR"
     gene            complement(2177..3802)
                     /locus_tag="VEL74_01045"
     CDS             complement(2177..3802)
                     /locus_tag="VEL74_01045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG61140.1"
                     /translation="MAEPREERILILAPTGRDAALACQILGQQGLSAHACASEQELFR
                     EIDAGAGLVILAEEALLPWTVNGLREALKKQEPWSDLPLVVFSRGGDSLNVLETLGPL
                     SNATILERPVRVSSLASAVQAALRARRRQYEVRDLLRRLADADRRKDEFLAMLGHELR
                     NPLSAIRNALWVLDEVGSREEPAVRQREVIARQATHLTRMVDDLLDVSRVTLGKIILK
                     HQAVDLEEVTVRCLQELGMASLAETQGLEVEVRTEPAVVQGDPVRLEQVVCNLFQNAI
                     KYTPRGGKLEITVGPDGGEATVRVRDTGVGIATEMLPRIFEPFTQVESSRQRSEGGLG
                     LGLGLVRSLVEMHGGRVEAASDGPGRGSEFTVRLPLHQPVRIARPIPFPVPAQAPEEA
                     AQAPVFELRNDHRAHGGKLHVLVIEDNPDGRESMRDLLEIWGHRVDLAENGVTGLEKA
                     LATQPDVALVDIGLPGMDGNEVARRIRSVYDGNQMALIAMTGYGQPEDRRRALQAGFD
                     SYLVKPVDPAELARLLTEVGRAPLGETLAEQVM"
     gene            complement(3795..5291)
                     /locus_tag="VEL74_01050"
     CDS             complement(3795..5291)
                     /locus_tag="VEL74_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HYG61141.1"
                     /translation="MSREASHLLDRAATGIAGMDEILGGGFPRNRMYLVEGDPGAGKT
                     TLALQFLLEGARRGEAGVYVTLSETEEELRDVARSHGWTLDGITICDLQTSEESLQAD
                     SQYTLFHPSEIELSETTRSVLEVVEKVNPTRVVFDSLSEMRLLARDSLRYRRQVLSLK
                     QYFTQRNCTVLLLDYDASTSGDFQLQSLTHGVIALEQLAPEYGGQRRRLRIKKVRGVR
                     FHDGFHDFRIETGGLVIYPRLVAAGHQRSFQRETLASGLPELDQLLGGGVDRGTSLML
                     LGPSGVGKTVITSQYVTAAAARGEPSAIFLFDESPDTWRIRAEGLGLDVGQHLESGTI
                     SLRQIDPAEMAPGEFAQAVRQAVDTGCRIVVIDSLNGYRHAMPEERFLTLHLHELLSH
                     LNQQGVVTFMVLAQHGLVGSDLESAIELTYLSDNIIVVRYFEAFGSVRQAISVVKKRT
                     GNHERSVRELRLGPGIQVGNQLREFQGILSGQNLIFNDREQLLGDGHG"
     gene            complement(5453..>5957)
                     /locus_tag="VEL74_01055"
     CDS             complement(5453..>5957)
                     /locus_tag="VEL74_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HYG61142.1"
                     /translation="RFQVHDRPVTFTDRVYGPLTERVDETVGDFVLKRRDGLWAYQLA
                     VVVDDLAMGIDDVVRGADLLSSTARQIQLIEALGGEPPAYAHVPLVVNARGEKLSKRD
                     AGLTLRSLRSAGLFPAAVTGYLAWSLGLLDRPEPCRPADLLPVFSWQRIGREDWVLPE
                     DPAPGIR"
BASE COUNT          852 a         2123 c         2039 g          943 t
ORIGIN      
        1 atagcggtgc tcgaggtcga cgcgcgcctt ctcgagatct cgctcggcct ggttcagcca
       61 gtcgcggctc cgctccatgg ccggattcta tccccgccgg atacggatcg accagggctc
      121 gtgttaccca cagtgtcctc ttcagccgtt ggcctcgcac cctgtctctg cgataccctg
      181 taggcaagga tgcgatccga ctatcaagtc ttcaagctct ggttcatggc cgtcctgccg
      241 ctggccaagg acgcgatcca tgtctatgtg ggcttcggct gcctgctggt cgccctgctc
      301 ctgctgcgcc tcaagctgtc ctcgttctgg tgcctggtgc ccggcctggt ggtgtcgctg
      361 atcatggagg ccctcgacct gcgcgacggc tacgcctggg gggagagcct caaggacctc
      421 atcaacacca acgccatccc gttcatcctg gtcgccctgg cccgctggcg ggtgttcaac
      481 atctattgat ggcctgactc tctcgggagc ccgtatggag aaggtggacc actggcagag
      541 cctctggggg cagacgtacg ccgacaagcc tacggagggg gatcccaccc tggtcctctc
      601 gggctggaac agcagctaca ccggcaagcc gatccgcccc gacgagatga aggactgggt
      661 ggagcgcacg gtcgagcgca tcctcgcccc ctccccccgc cgcgtcctgg agctcggctg
      721 cggcatgggc ctcctcctct tccgggtcgc ccccaaggtg ggcgcctact ggggcgtcga
      781 cttctcggcc gaggccctcg cccacgtcgg cgagcacgcc gcccgcctcg gcctcgccaa
      841 cgtcgagctc ctccagcagg gggccgacga cttcacccgc ctgcgccagg cggagccgtt
      901 cgacacgctg gtgatcaact cggtcctcca gtacttcccg gacatggact acctgctgcg
      961 cgtcctcgag agcgcccagg agatcctcgt gcccgggggc cggatcttcc tgggggacat
     1021 ccgcagcctg ccgctgctgt cggccttcca cgcctcggtc cagatgctcc aggcggacgg
     1081 cacgacgccg ctcgccgagc tgcgggagcg ggtggagaag ctgacccgcg aggaacggga
     1141 gctggcgctc gcccccagct tcttcgccga gctggctgat ctccggcggc gcttcccgcg
     1201 catcacccgg gtggagatcc agctcaagcg cggccgctac cgcaacgagc tgacccgctt
     1261 ccggtacgac gccgtcctct ggctggacgg ctccggggcc gtgcccgatc ccgagagctg
     1321 gctcgactgg cgcggcgagg ggctgacgct gccggacctc aagcgccgcc tcgcccagga
     1381 gggccccgcc ctcctcggcg tgcgcggcat ccccaatgcc cgcatcctcc ccgagacccg
     1441 cgccgtcgag ctgatccagg cgggcgcctc ggaggccggg ccgctgcaca cggtggacga
     1501 cctgctggcc gccatgcgcg gggtgcgcgg cgggatcgag cccgaggacc tgtgggcgct
     1561 gagcaagacg ctgccgtacg acatcgaggt cggctggggg gaagaccacc ccgacggcgc
     1621 cttccaggcc gtcttccggc ggcgctaggc cttcacccca cggctgcctc tcccggtccg
     1681 cccattcgta gatgaaacag cgccttcccc gccgtcgcgg gggaggcgct tttctcttgg
     1741 gggagttttc ggcggcggtt cggaagcttc cggcggcttg gcgaagagta cgacggcgcg
     1801 tcagaggttc caatggccca gcggagactc cggcagcctg tcgaagacac cggcgaagcg
     1861 ccggaagctt cggccacccg gcgcagactc cggcaacgcg ccgggagctt cgacagctcg
     1921 ctggaagctt ccgttggccc gtcagagttt gcggcggccc gccgggacct ctgacggcgc
     1981 gccggaagct tcggcagcgc accggaagct tcggccgtcc gtcggaagct ctgacagcgc
     2041 gtcggaaact ctgataccgc gtcggaagct tgcgacggct cgtcggagac tccgacagcc
     2101 tgtcggatcg tatgacggcg tctgtacgcc tacgccgctt cgcggctgga gatccctgag
     2161 gcctgaggcc ggctgctcac atcacctgtt cggcgagggt ctccccgagc ggggcgcggc
     2221 ccacctcggt gagcaggcgc gccagctcgg cggggtccac cggcttgacc agatacgagt
     2281 cgaagccggc ctggagggcg cggcggcggt cctccggctg gccgtagccg gtcatggcga
     2341 tgagcgccat ctggttgccg tcgtagaccg agcggatgcg gcgcgccacc tcgttgccgt
     2401 ccatcccggg cagcccgatg tcgaccagcg ccacgtcggg ctgggtggcg agcgccttct
     2461 ccaggccggt cacgccgttc tcggccaggt ccacccggtg cccccagatc tccagcaagt
     2521 cgcgcatcga ctcgcggccg tccgggttgt cctcgatcac caggacgtgc agcttgccac
     2581 cgtgcgcgcg gtggtcgttg cggagctcga agacgggggc ctgcgcggcc tcctctggag
     2641 cctgggccgg taccgggaag gggatcgggc gggcgatccg gacgggctgg tggaggggca
     2701 gccgcacggt gaactcgctc ccccggcccg gcccgtcgct cgcggcctcc acccgtccgc
     2761 cgtgcatctc gacgaggctg cggaccaggc cgaggccgag cccgagcccg ccctcggagc
     2821 gctgccggga gctctccact tgggtgaacg gctcgaagat gcgcggcagc atctccgtgg
     2881 cgataccgac gcccgtgtcc cgcacccgca ccgtggcctc gccaccgtcc ggtccgaccg
     2941 tgatctcgag cttgccgccg cggggcgtgt acttgatcgc gttctgaaag aggttgcaga
     3001 ccacctgctc gaggcgcacg ggatccccct ggacgaccgc cggctccgtc cgcacttcca
     3061 cctccagtcc ctgtgtctcc gccagggagg ccatgcccag ctcctgcagg cagcggacgg
     3121 tcacctcctc gaggtcgacc gcctggtgct tgaggatgat cttgcccagg gtcacccggg
     3181 agacgtccag caggtcgtcc accatgcggg tgaggtgagt ggcctggcgg gcgatgacct
     3241 cccgctgccg cacggccggc tcctcgcgcg agccgacctc gtccagcacc cagagggcat
     3301 tgcggatcgc cgacaggggg ttgcgcagct cgtggccgag catcgccagg aactcgtcct
     3361 tgcggcggtc ggcgtcggcg agccggcgca gcaggtcgcg cacctcgtac tggcggcggc
     3421 gggcgcgcaa cgccgcctgc acggcgctcg ccagcgagct cacccgcacc gggcgctcga
     3481 ggatggtggc gttgctgagc ggccccagcg tctcgagcac gttcaggctg tcgccgccgc
     3541 ggctgaagac caccagcggc aggtccgacc agggctcctg cttcttaagc gcctcccgca
     3601 gcccgttgac ggtccacggc aacagggcct cctcggccag gatcaccagc cccgcccccg
     3661 catcgatctc ccggaagagc tcctgttcgc tcgcgcaggc gtgggcggac agcccctgct
     3721 gcccgaggat ctggcaggcc agggcggcgt cgcgcccggt gggggccagg atgaggatcc
     3781 gttcttctcg cggctcagcc atgtccatcc ccgaggagtt gctcgcggtc gttgaagatc
     3841 aggttttgtc cgctcaggat gccctggaac tcccgcaact ggttcccgac ctggatgccg
     3901 ggccccaggc gcagctcgcg gacgctccgc tcgtggttgc cggtccgctt cttgaccacc
     3961 gagatcgcct ggcgcacgct gccgaaagcc tcgaagtagc ggacgacgat gatgttgtcc
     4021 gacagatagg tcagctcgat ggccgactcc aggtccgacc ccaccaggcc gtgctgggcg
     4081 aggaccatga aggtgacgac gccctgctgg ttgaggtgcg acaggagctc gtgcaggtgg
     4141 agggtcagga agcgctcctc cggcatcgcg tggcgatagc cgttcaggct gtcgatcacc
     4201 acgatccggc agccggtgtc caccgcctgc cgcacggcct gggcgaactc gccgggcgcc
     4261 atctccgccg ggtcgatctg gcgcagggag atcgtgccgc tttccaggtg ctgaccgacg
     4321 tccaggccga gaccttcggc gcggatccgc caggtgtccg gcgactcgtc gaagaggaag
     4381 atcgccgacg gctcgccgcg cgccgccgcg gccgtgacgt actgcgaggt gatcacggtc
     4441 ttgcctacgc ccgaaggccc cagcagcatg aggctggtac cccggtccac gccgcctccc
     4501 aggagctggt cgagctccgg caggccgctc gccagggtct cgcgctggaa cgagcgctgg
     4561 tgcccggccg cgacgagccg cggatagatg acgagcccgc cggtctcgat cctgaaatcg
     4621 tggaagccgt cgtggaagcg gacgccgcgt accttcttga tccgcagccg gcggcgctgg
     4681 ccgccgtact cgggggccag ctgctccagc gcgatcaccc cgtgcgtcag gctctggagc
     4741 tggaagtcgc ccgacgtcga cgcgtcgtag tcgagcagca ggacggtgca gttgcgttgc
     4801 gtgaagtact gcttcaggga cagcacctgg cgccggtagc gcagggagtc gcgggcgagc
     4861 aggcgcatct ccgacagcga gtcgaacacc acccgcgtcg ggttgacctt ctccaccacc
     4921 tcgagcaccg agcgcgtggt ctccgacagc tcgatctccg acgggtggaa cagggtgtat
     4981 tgggagtcgg cctgcaggct ctcctccgag gtctggaggt cgcagatggt gatgccgtcg
     5041 agcgtccagc cgtgagagcg ggcgacgtcg cgcagctcct cttcggtctc cgacagcgtg
     5101 acgtagaccc cggcctcgcc gcgccgcgcc ccctcgagga ggaattggag tgcgagggtg
     5161 gtcttgcccg cgcccgggtc cccctcgacc aggtacatgc ggttgcgggg gaatcctccc
     5221 ccgaggatct cgtccatgcc cgcgatgccg gtcgctgcac ggtcgagaag gtgagatgcc
     5281 tctcggctca tgatggtcta tgtctcctca gttcgaggag aggcggccag gcagggaacc
     5341 catgggtcgc atcttctata ggccgccgct ccaagagtct gcgctccgat ctggcgtcgc
     5401 aggccgcggc ggatcgaggg tgcgttgtgc aaggtgaatg ccacaggaaa tcttatcgaa
     5461 tcccgggcgc cggatcctcg ggcagcaccc agtcctcccg gcctattctc tgccaggaaa
     5521 agaccgggag caggtcggcg ggccggcagg gctcgggacg gtcgagcagc cccagcgacc
     5581 aggcgaggta gccggtcacg gctgcgggga acaggcccgc cgaccgcaac gagcgcaggg
     5641 tcagccccgc gtcgcgcttc gacagcttct ctccccgggc gttgaccacc aggggcacgt
     5701 gggcgtacgc cggcggctcg cccccgagcg cctcgatgag ctggatctgc cgcgccgtgg
     5761 aggagagcag gtccgccccc cgcaccacgt cgtcgatccc catcgccaag tcgtccacca
     5821 ccaccgcgag ctggtacgcc cagagtccgt cgcggcgctt cagcacgaag tcgccgacgg
     5881 tctcgtccac ccgctcggtc agcgggccgt agacccggtc cgtgaaggtg accggccggt
     5941 cgtgcacctg gaagcgg
//
LOCUS       DASPJI010000038         2720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_574223, whole genome shotgun sequence.
ACCESSION   DASPJI010000038 DASPJI010000000
VERSION     DASPJI010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574223"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..469
                     /locus_tag="VEL74_01060"
     CDS             191..469
                     /locus_tag="VEL74_01060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG61143.1"
                     /translation="MELEVRHDEGARKYYALIDGRESVLEYSEAREGTLNFFHTYVPP
                     ELRGRGIADEIVRHALEDAERRGMKVIPSCWFVRVYIQRHSRYQPLVA"
     gene            484..765
                     /locus_tag="VEL74_01065"
     CDS             484..765
                     /locus_tag="VEL74_01065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG61144.1"
                     /translation="MDFEVQHEEGNKYYAVIDGSEAAVGYFKLDDETLDFQHTYVPEK
                     LRGQGVAEKLVRHALDDARRRRLRVLPSCPYVKRFVERHPEYQQGLARA"
     gene            complement(762..1772)
                     /locus_tag="VEL74_01070"
     CDS             complement(762..1772)
                     /locus_tag="VEL74_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61145.1"
                     /translation="MTPMPRLRILLVLPLLLLGALAVPETASAAEKLALRTNDAEGVP
                     GGVVAVVVRTYASRAIGQGQILFRSGGLRTKAIEASKRRGRGGEGEGEGGGSGPSGPS
                     PFVALESFVVFSARQDARFQGSFDTRRATLSFNSPSRTINSADGPLAIFYFRLADWVQ
                     PGQVYEIGIDPGTTLVDDKGRRVQVEPRNGELEIVADTGAQELEVEPTGVVKPGRMAI
                     IGLETKMSEPLGSGRIGFRYDKSIARKPPFVRVDPRMGNVRFNADVSTPGLVLITFDS
                     TDGSFNRFPGAIFEVRIPTGKWVRRGTRAPLTLDPSLTFLVDREGDVLDLELENGTLI
                     FR"
     gene            complement(1778..2623)
                     /locus_tag="VEL74_01075"
     CDS             complement(1778..2623)
                     /locus_tag="VEL74_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYG61146.1"
                     /translation="MKDGTPQGLPLGEALRAAHSPEHGCPPPEAFLSWEAGDLSDEER
                     RRLDEHVAGCPACAAERDLARLFEPPSEDSEEMAARREDMDFILARLEKGRPASPRAV
                     REAKVVPFPAAAPARSPEMPMASALPAARRRSRFLPAMGLAAAAMLALVFGLLFQMRS
                     APPLPEPGAGGGGGVMRGADLAVVAPVGEIAAVPSELRWEEIPAAHSYRWRLLSVDGS
                     VVWEETASGSPVRLPQALVRSLHPAVAYTWTVEALDTQGDRIATSTPARFRVRPAGEN
                     GSTSR"
BASE COUNT          439 a          935 c          965 g          381 t
ORIGIN      
        1 agaacgatca gaaaactcgc aggacgtctc ataccttctc tctttctaca ggccttgaag
       61 ggttgatttt catgactgca cggccagtag aacgaagctc cgcagccatc cccctcacgg
      121 gttggcacgc tggttgaagc ccgttacgct ggagcgcccg cgacggggct acccgcccgg
      181 aggaggaacc atggagctgg aagtgcggca tgacgaaggc gccagaaagt attatgcgct
      241 aatcgacggc agggagtcgg tgctcgagta ctccgaggcg cgcgagggga ccctcaattt
      301 cttccacacc tatgtgccgc cggagctgcg gggccgcggc atcgccgacg agatcgtccg
      361 ccatgccctc gaggacgcgg agaggcgcgg gatgaaggtc atcccgtcct gctggttcgt
      421 ccgcgtctac atccagagac attcgcgtta ccagccgctg gtcgcctaga gagaggaagc
      481 ctgatggatt tcgaagtcca gcacgaggaa gggaacaagt actacgccgt gatcgacggc
      541 tccgaggccg ccgtggggta cttcaagctc gacgacgaga cgctcgactt ccagcacacc
      601 tacgtgccgg agaagctgcg cggccagggg gtggccgaga agctggtccg ccacgccctc
      661 gacgatgccc ggcggcggcg tcttcgggtg ctcccgagct gcccctacgt caagcggttc
      721 gtggagcggc acccggagta ccagcagggc ctggcccggg cctagcggaa aatcagggtt
      781 ccgttctcca gctccagatc caggacgtcc ccctcgcggt ccaccaggaa ggtgagcgag
      841 ggatccaggg tcagaggcgc ccgggtcccc cgccgcaccc acttgcccgt agggatccgc
      901 acctcgaaga tcgccccggg gaagcggttg aacgagccgt ccgtcgagtc gaaggtgatg
      961 aggacgaggc ccggcgtcga gacgtcggcg ttgaagcgca cgttgcccat ccgcgggtcg
     1021 acgcgcacga aggggggctt gcgggcgatc gacttgtcgt accggaagcc gatccggccg
     1081 ctgccgagag gctccgacat cttggtctcc aggccgatga tggccatgcg gcccggcttc
     1141 acgacgccgg tgggctccac ctccagctcc tgggcgccgg tgtcggccac gatctcgagc
     1201 tcgccattgc gcggctccac ctgcacgcgc cgccccttgt cgtcgaccag ggtggtgccg
     1261 ggatcgatcc cgatctcgta aacctgcccg ggctgcaccc agtcggccag ccggaaatag
     1321 aagatggcca gcggcccgtc ggcggagttg atagtgcgcg acggcgagtt gaagctgagg
     1381 gttgccctcc gggtgtcgaa gctcccctgg aagcgggcat cctgccgggc gctgaagacc
     1441 acgaagctct ccagcgcgac gaaggggctc gggccgctcg gaccggagcc gcccccctcg
     1501 ccttcaccct cgccaccccg tccccgccgc ttggacgcct cgatcgcctt ggtgcggagg
     1561 ccgccgctcc ggaacaggat ctgcccctgg ccgatggccc gcgacgcgta ggtgcggacc
     1621 accacggcga cgacgccgcc gggaaccccc tcggcgtcgt tggtgcgcag cgcgagcttt
     1681 tcggcggccg aggcggtctc gggaacggcc agggcaccga gcaggaggag cgggagaacg
     1741 agaaggatcc ggagacgcgg catgggtgtc atgggtctta cctcgaagtc gagccgttct
     1801 ctccggccgg ccgcacacgg aagcgggccg gcgtggaggt ggcgatccgg tcgccttggg
     1861 tgtccagggc ctccaccgtc caggtgtagg ccaccgcggg atggagactg cggaccagcg
     1921 cctgcggcag acggaccggc gaaccggagg ccgtctcttc ccatacgacc gagccgtcga
     1981 cagagagcag gcgccaacga taactgtggg ccgcgggaat ttcttcccac cgcagctcgg
     2041 acgggacggc ggcgatctcg ccgacggggg cgacgacggc caggtccgcg ccgcgcatca
     2101 cgccgcctcc gccgcccgcg ccgggctcgg ggagcggcgg cgccgaccgc atctgaaaga
     2161 ggaggccgaa gaccagggcc agcatggccg cggcggccag gcccatggcc ggcaggaaac
     2221 gggaccggcg gcgcgccgcc gggagggcgg acgccatggg catctcggga gaccgggcgg
     2281 gcgcggccgc cgggaagggc accaccttcg cctcgcgcac ggccctggga gatgcgggac
     2341 gccccttctc caggcgggcg aggatgaaat ccatgtcctc gcgccgggcc gccatctcct
     2401 cgctgtcctc cgacggcggc tcgaagaggc gggcgaggtc gcgctcggcg gcgcaggccg
     2461 ggcaacccgc cacgtgctcg tcgagacggc ggcgctcctc gtccgacagg tcgccggcct
     2521 cccagctcag aaaggcctcg gggggtgggc agccatgctc gggcgagtgg gccgcccgca
     2581 gcgcctcacc gagcgggagc ccctggggtg tgccgtcctt catggctttc ctccgccggc
     2641 cgccgccggc tgtgcggacg ctgccagccg gcgccgcagc ttgcgcaagc cgctgaccac
     2701 gtaggccttc gctcccgatt
//
LOCUS       DASPJI010000039         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_2223, whole genome shotgun sequence.
ACCESSION   DASPJI010000039 DASPJI010000000
VERSION     DASPJI010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2223"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..601)
                     /locus_tag="VEL74_01080"
     CDS             complement(110..601)
                     /locus_tag="VEL74_01080"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HYG61147.1"
                     /translation="MVVIIMGVSGSGKTTVGRLLAAELGWSFHEGDSLHPRANLEKMA
                     AGLPLDDGDRLPWLHVLRELIDRCLASGEGAVLTCSALRRSYREILGTGRPGVALVHL
                     QGSFEQIDRRLAARQGHFMPRSLLASQLAALEAPAEGEALSIPVDREPAEIVREIRVR
                     LRL"
     gene            complement(611..1672)
                     /locus_tag="VEL74_01085"
     CDS             complement(611..1672)
                     /locus_tag="VEL74_01085"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HYG61148.1"
                     /translation="MITETTRDCYAALTRLVQDGGKAGRWLVLTHDNPDPDALASALI
                     LTRVLRGAFKQKVTMAYGGIIGRAENREMVRSLRLELSHVRNLNFKSYQRFALVDTQP
                     RTGNNQLPARITPDIVIDHHPVRQNTQAGPFFDVRPPYGATATILAEYLLASGAKPTQ
                     AVATALIYAIRSETRDFAREYTGPDKAVYDHFFPQANHRLLARIQSPRLPLTYFSNLH
                     EALEKLLEADTLIVSHLGNVEQSDIVPEIADLLLRMEGKTWSLCTGIFSDRLYLSIRT
                     TNTRADAGAVMHRLVGRRGKGGGHGMMAGGWVDLSKAGDEERRKLQRQLGERLARELK
                     KNPDKMTPISLGAQRKPGP"
     gene            1758..>2117
                     /locus_tag="VEL74_01090"
     CDS             1758..>2117
                     /locus_tag="VEL74_01090"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HYG61149.1"
                     /translation="MIDEVFRIGSFAISPFGLMLVFAFLASYFQLRWGMQRLNIGDEE
                     DASALIFAAGVGGIVGAKVYYAILTQSWRVLFDRSGLVWYGGFILGAIAVLLVMRWRR
                     IPAWPLVDASAPALALGY"
BASE COUNT          262 a          677 c          823 g          355 t
ORIGIN      
        1 cggggtatgg cggccgtacg cccggggatg atctccgtct ccgcgggtat ggcggctggt
       61 tcgaggggaa aaacgcccgc gctgtccgcg atgccgccgg ggcaccctct cagagccgga
      121 ggcggacgcg gatctcccgt acgatctcgg cgggctcgcg gtccacgggg atcgacaggg
      181 cctccccctc ggcgggcgct tccagggccg cgagctggct cgccaggagc gagcggggca
      241 tgaagtggcc ctgccgcgcg gcgagccggc ggtcgatctg ctcgaagctg ccctggaggt
      301 ggaccagcgc caccccgggg cggccggtgc cgaggatttc gcggtaggag cgccggaggg
      361 cggagcaggt gaggaccgcc ccctctcccg acgcgaggca gcggtcgatc agctcgcgca
      421 ggacgtggag ccaggggagg cggtcgccgt cgtccagggg gaggccggca gccatcttct
      481 cgaggttggc acgggggtga aggctgtccc cctcgtggaa gctccacccc agctcggcgg
      541 cgaggaggcg ccccacggtc gtcttgccgc tgccggagac gcccatgatg atgaccacca
      601 tgagaggagg tcagggtccc ggcttgcgct gggcgcccag ggagatcggg gtcatcttgt
      661 cggggttctt cttgagctcg cgcgccagcc gctcgccgag ctggcgctgg agcttgcggc
      721 gctcctcgtc gccggccttg ctgaggtcga cccagccgcc ggccatcatg ccgtgcccgc
      781 cccccttgcc gcggcggccg acgaggcggt gcatgacggc gccggcgtcg gcccgggtgt
      841 tggtggtgcg gatcgacagg tagaggcggt cgctgaagat gccggtgcag agcgaccagg
      901 tcttgccctc catgcgcagc agcaggtcgg cgatctcggg cacgatgtcc gactgctcca
      961 cgttgccgag atggctgacg atcagggtgt cggcctcgag gagcttctcc agcgcctcgt
     1021 ggaggttgct gaagtaggtg agcggcaggc gggggctctg gatgcgggcg aggaggcggt
     1081 ggttcgcctg cgggaagaag tggtcgtaga ccgccttgtc cgggccggtg tactcgcggg
     1141 cgaagtcccg cgtctccgag cggatggcgt agatcagggc ggtggcgacg gcctgggtgg
     1201 gcttggcgcc gctcgccagg aggtactcgg ccaggatggt ggcggtggcg ccgtagggcg
     1261 gccggacgtc gaagaagggt ccggcctggg tgttctgccg cactgggtgg tggtcgatga
     1321 cgatgtcggg ggtgatccgc gcggggagct ggttgttgcc ggtgcggggc tgcgtgtcga
     1381 ccagggcgaa gcgctggtag ctcttgaagt tgaggttgcg gacgtggctg agctcgaggc
     1441 gcaggctgcg caccatctcg cggttctcgg cgcggccgat gatgccgccg taggccatgg
     1501 tgaccttctg cttgaaggcg ccgcgcagca cccgggtgag gatcagggcg gaggccaggg
     1561 cgtccgggtc cgggttgtcg tgggtgagga ccagccagcg gccggccttg ccgccgtcct
     1621 ggacgagccg ggtaagggcc gcgtagcagt ccctggtggt ctccgtgatc atgggagggc
     1681 cgattctact gcacgtgcta ccattccccg gcccgcccgg cctcctgtcc ggtagcctgc
     1741 ccgggggcaa cccctttatg atcgacgagg tcttccgcat cggctcattc gccatcagcc
     1801 ccttcgggct gatgctggtc ttcgccttcc tggcgagcta tttccagctc cgctggggca
     1861 tgcagcgcct gaacatcggc gacgaggagg acgcgagcgc cctcatcttc gccgccggcg
     1921 tcggcggcat cgtcggcgcc aaggtctact acgccatcct cacccagagc tggcgggtgc
     1981 tcttcgaccg ctccggcctg gtctggtacg gcggcttcat cctcggcgcc atcgcggtgc
     2041 tgctggtgat gcgctggcgc cggatcccgg cctggccgct ggtggacgcg tcggcgccgg
     2101 ccctcgccct cggctac
//
LOCUS       DASPJI010000040         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_517318, whole genome shotgun sequence.
ACCESSION   DASPJI010000040 DASPJI010000000
VERSION     DASPJI010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517318"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..633
                     /locus_tag="VEL74_01095"
     CDS             148..633
                     /locus_tag="VEL74_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYG61150.1"
                     /translation="MSEDRSLRNHLLYLLRGGGAHAHFEQVIADLPAELRGVRPQGAV
                     HSAWEVLEHLRITQWDILEFSRNAGHVSPDWPEGYWPKEQAPPDGRAWDCTLEAFRQD
                     LKAMQELVDDAGTDLYARIPWGDGQTILREALLVADHNAYHLGELVLLRRLLGAWPPA
                     E"
     gene            692..943
                     /locus_tag="VEL74_01100"
     CDS             692..943
                     /locus_tag="VEL74_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HYG61151.1"
                     /translation="MQNHHYKFDRITFDPDQCSGKACIRGMRMPVASILAYLGSGMTQ
                     EQILEEWPELEGEDLHQALAYAASVLEERVVPLVESARP"
     gene            940..1317
                     /locus_tag="VEL74_01105"
     CDS             940..1317
                     /locus_tag="VEL74_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HYG61152.1"
                     /translation="MNFLLDMNLPRQLGRLLAGRGHGWRHVADIGFYKAMDKEILAEA
                     KIRGEVILTHDLDYGTLLAFSGESEPSVAIFRQSDILPSALFESMMRNWHECGPALEA
                     GAIVIFEDTAIRIRPLPIGRGLS"
     gene            complement(1382..2308)
                     /locus_tag="VEL74_01110"
     CDS             complement(1382..2308)
                     /locus_tag="VEL74_01110"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYG61153.1"
                     /translation="MGMSEFYGQTDDSESIATIHRALELGVTFLDTAEMYGQGRNEEL
                     VGRALAGRRAGVVLATKFGVVRNKETGAIVGVDGRPEVVRPSCEGSLRRLGTDHIDLY
                     YLHRVDPEVPIEETVGAMKELVDEGKVRFLGLSEASEETIRRAHEVYPITALQTEYSL
                     WSREPEDEIFPVLRELGIGFVPYSPLGRGFLTGQIQSFDDLAPDDYRRTAPRFQGENF
                     AKNLELVHAIQDMARRKGCTPAQLALAWVLAQDPDFAPIPGTKRRDRLEENVGSLSVD
                     LSEEELRQIDEIAPKGVAAGERYSRPVIQSTK"
BASE COUNT          351 a          794 c          778 g          387 t
ORIGIN      
        1 ccccggccgg gccggcggcg gcgaggagct gggcgggctc aatggcgtcc ggctgtacct
       61 ccagcgcgtg gccctccagg ggagtcgtac cctggtggac gacctcgccg ggacaacgcc
      121 cgctccggcc gccgcggggg acgctcggtg agcgaggacc gctctcttcg caaccacctc
      181 ctctatctgt tgcggggagg cggtgcccat gcgcatttcg agcaggtgat cgccgacctc
      241 ccggcggagc tgcgcggcgt ccgccctcag ggcgccgttc actccgcctg ggaggtcctg
      301 gagcacctgc ggatcaccca gtgggacatc ctggagttca gccgcaacgc cggtcacgtc
      361 tccccggact ggccggaggg ctactggccg aaggagcagg ctccgcccga cggccgggcc
      421 tgggactgta ccctcgaagc cttccggcag gacctcaagg ccatgcagga gctggtggac
      481 gacgccggaa ccgacctcta cgcccgcatc ccgtgggggg acggccagac catcctccgc
      541 gaagccctcc tggtggccga ccacaacgcc taccatctgg gagagctggt gctgctgcgg
      601 cgcctgctgg gggcttggcc tccggcggag taggggaggg gggacgctgc gaggcccgct
      661 gtcgttgtat cctctggcga gaggctaggc catgcagaac catcactaca agttcgaccg
      721 catcaccttc gatccggatc agtgctccgg gaaggcgtgc atccgaggga tgaggatgcc
      781 ggtcgcctcg atcctggcct atttaggctc cggcatgacc caggagcaga tcctggaaga
      841 gtggccggag ctcgaaggtg aggatctcca ccaggcgctg gcttatgcgg cctccgtcct
      901 cgaagagcgg gtcgtgcctc tcgtcgagtc cgcacggcct tgaacttcct cctggacatg
      961 aatctccctc ggcagctcgg ccgccttttg gccgggcgtg ggcacggctg gaggcatgtc
     1021 gccgacatcg gcttctacaa agcgatggac aaggagatcc tggccgaagc caagattcgt
     1081 ggcgaggtca tcctcactca cgacctggac tacggaaccc tgctggcttt ctcaggagag
     1141 tcggagccat ccgtggcgat cttccggcag agcgacattc ttccgtctgc tctcttcgag
     1201 tcgatgatga ggaactggca cgaatgtggg ccggctctgg aagcgggggc catcgtcatc
     1261 tttgaggaca cggcgatccg aatccgtcct ctccccatcg gccgtgggct gagctgatcg
     1321 ggctcgtata tcaaagggta gccgggcgcc ttcgctcagg cgcccggcca gccctccccc
     1381 ctcacttcgt gctctggatc accggccggg agtagcgctc gccggcggcc acgcccttcg
     1441 gggcgatctc gtcgatctgc cgcagctcct cttccgaaag atcgaccgac agcgagccga
     1501 cgttctcctc caggcggtcg cggcgcttgg tgccggggat gggggcgaag tcggggtcct
     1561 gagccagcac ccaggcgagg gcgagctggg ccggggtgca tcccttccgg cgcgccatgt
     1621 cctggatggc atggacgagc tccaggttct tggcgaagtt ctcgccctgg aagcgggggg
     1681 ccgtccgccg gtaatcgtcc ggcgccaggt cgtcgaacga ctggatctga cccgtgagga
     1741 agccccggcc cagcgggctg tagggcacga agccgatgcc cagctcccgg agcacgggga
     1801 agatctcgtc ctcgggctcc cggctccaca gcgagtactc ggtctggagc gccgtgatgg
     1861 ggtagacctc gtgggcccgg cggatcgtct cctccgatgc ctccgagagt ccgaggaagc
     1921 ggaccttccc ctcgtccacc agctccttca tggccccgac cgtctcctcg atggggacct
     1981 ccgggtccac acgatgcagg tagtagaggt cgatgtggtc ggtgccgagc cggcgcagcg
     2041 acccttcgca ggacggacgc accacctccg gccgcccgtc gaccccgacg atggcacccg
     2101 tctccttatt ccgtacgacg ccgaacttgg tggcgagcac caccccggcg cgccgccccg
     2161 caagggcgcg gccgaccagc tcctcgttgc ggccctggcc gtacatctcg gccgtgtcga
     2221 ggaacgtcac cccgagctcg agcgcccggt ggatcgtggc gatcgactcg gagtcgtccg
     2281 tctggccgta gaactccgac atccccatgc
//
LOCUS       DASPJI010000041         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_461544, whole genome shotgun sequence.
ACCESSION   DASPJI010000041 DASPJI010000000
VERSION     DASPJI010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461544"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2540
                     /locus_tag="VEL74_01115"
     CDS             <1..>2540
                     /locus_tag="VEL74_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61154.1"
                     /translation="RSLSHSPLFQVLFVLLHQGPEAKVSPKGLVTGPFPAERGTSKFD
                     LMLALYAAGEAFTGTIEYATDLFDRPTIARLAEHLRTLASAVSAAPDGLLSELPLLGE
                     AERHQLLAEWNDTAQPLPDLSLHHLFERQAERTPDAAAASFADHWLTYAELARRAARL
                     ARHLRRLGIGPESRVGLGVDRSFEMVTGVLGVLTAGAAYVPLDPALPRERRAYMLENA
                     GVEVLLTRSGLLDEMSQDGVRSLCLDGPALEGPVVPPAAHPPDPGCLAYVIYTSGSTG
                     RPKGVGLPRLALSNLIDWHLRHFLGGVRTLQFASLSFDVSFLEMFACWGSGGTLLLVS
                     EELRRDVPALARFLVEAEAEKAIFPVVILQRLAEEYAGRGFRPLPPLAEITTMGEQLQ
                     TTRVMGELLRRLGGCAFHNHYGPSESHVVTAFALDPGPASWATHPPIGLAIANTRVHL
                     LDERLAPVPVGVPGELAIGGVCLARGYAGRPDLTAEKFVPDPFAGLGHEPGARLYRTG
                     DKVRRLPDGNLDFLGRFDHQVKVRGFRVEPEEIESVLGGCPGVRQTVVLARRMGAGDT
                     RLVAYIVPEPGAGSPGIWRTFLRERLPDYMVPSHFVVLEALPLNANGKVDRGVLPAPA
                     EETARPELAAVYAAPRTPIEETLAAIWSDVLGIERVGVEDGFFELGGQSLLATQAMSR
                     VRQAFGVEVPLRALFEGPTVAELARAVEAARAGLAMAGPEAPPLVRMPASARAGELPL
                     SFAQQRLWFLDLLVPYNPFYNMFGAVRLSGPVDVPALREACREIARRHEVLRTTFSSV
                     AGRPVQVIAAEPPPGFPLIDLGGLTEAARRKELARLALAEGRRPFDLTR"
BASE COUNT          297 a          938 c          909 g          396 t
ORIGIN      
        1 cgcagcctca gccactcgcc gctcttccag gtgctgttcg tcctcttgca tcaaggcccg
       61 gaggccaagg tctcaccgaa ggggctcgtg accggcccct tcccggccga gcgggggacc
      121 tccaaattcg acctcatgct cgcgctctac gcggctggcg aggcgttcac cggcacgatc
      181 gagtacgcca cggacctctt cgaccggccg accatcgcac ggctcgcgga gcacctgcgg
      241 accttggcgt cggccgtctc ggccgcgccg gacgggctgc tctccgagct gcccctgctc
      301 ggcgaggccg agcggcacca gctcctggcc gagtggaacg acaccgcgca gccgttgccg
      361 gacctgtcgc tccaccacct cttcgaacgg caggcggagc ggacgccgga tgccgcggcg
      421 gcgagcttcg ccgaccactg gctgacctat gccgaactgg cgcggcgggc ggcacgtctg
      481 gcccgccacc tgcgccgcct ggggatcggc cccgagtccc gagtggggct gggcgtggac
      541 cgctcgttcg agatggtgac gggagtcctc ggagtcctca cggccggggc cgcctacgtg
      601 cctctggacc ccgccctccc ccgggagagg cgcgcctaca tgctggagaa cgccggcgtc
      661 gaggtcctgc tgacgcgctc ggggttgctc gatgagatga gccaggacgg ggtgcggagc
      721 ctttgcctcg acggtcccgc cctggagggg ccggtggtcc cgccggcggc tcaccctccg
      781 gaccccggct gcctcgccta cgtcatctat acctcgggct ccacgggcag acccaagggg
      841 gtcgggctgc cgcgcctcgc gctctcgaac ctgatcgact ggcacctccg tcacttcctg
      901 ggcggcgtcc gcaccctgca gttcgcttcg ctgagcttcg acgtgagctt cctcgagatg
      961 ttcgcctgct ggggctcggg tgggaccctc ctcctcgttt ccgaggagct gcggcgggac
     1021 gtgccggccc tggcgcgctt tctcgtcgag gcggaggccg agaaggcgat cttcccggtc
     1081 gtgatcctcc agcgcctggc cgaggagtac gccgggcgcg gcttccgtcc gctgccgccg
     1141 ctcgccgaga tcaccaccat gggcgagcag ctccagacca cccgggtgat gggggagctg
     1201 ctgcggcgcc tcggcggctg tgccttccac aaccactacg gcccgtccga gagccacgtc
     1261 gtcaccgcct tcgccctcga tcccggcccg gcgagctggg cgacccaccc gccgatcggc
     1321 ctcgcgatcg ccaacacgcg cgtccatctg ctcgacgagc ggctcgcgcc cgtaccggtg
     1381 ggcgtccccg gcgagctggc gatcggcggc gtctgcctgg cgcgcggcta cgccggccgt
     1441 cccgacctga cggcggagaa gttcgtgccg gacccattcg ccggcttggg gcacgagccc
     1501 ggagcgcgtc tctaccggac cggcgacaag gtccgccggc tccctgacgg aaacctggac
     1561 ttcctcggcc gtttcgacca ccaggtgaag gtgcgcggct tccgcgtcga gccggaggag
     1621 atcgagtcgg tgctcggcgg ctgcccgggc gtgcggcaga cggtggtgct ggcgcggcgg
     1681 atgggtgccg gggacacgcg cctggtcgcc tacatcgtgc cggagcccgg tgccggttcg
     1741 ccgggtatct ggcggacctt cctgcgcgag aggctgccgg actacatggt cccctcgcat
     1801 ttcgtggtgc tggaggcctt gccgctcaac gccaacggca aggtggaccg cggggtgctc
     1861 cccgcgccgg cggaggagac ggcgcggccg gagctcgcgg cggtctacgc cgcgccacgc
     1921 acgcccatcg aggagaccct ggcggcgatc tggagcgacg tgctggggat cgagcgcgtc
     1981 ggggtcgagg acggcttctt cgagctcggc ggtcagtcgc tcctcgccac ccaggcgatg
     2041 tcgcgggtgc gccaggcgtt cggtgtcgag gtgcccctgc gcgcgctctt cgaggggccg
     2101 accgtcgccg agctggcgcg cgccgtcgag gcggcgcgcg cgggcctggc aatggccggt
     2161 ccggaggcgc cccccctggt gaggatgccg gcctcggcgc gggccggcga gctgccgctc
     2221 tcttttgcgc agcagcgcct ctggttcctc gacctattgg tgccgtacaa ccccttctac
     2281 aacatgttcg gcgcggtgcg gctctccggt ccggtggatg tgccggccct gcgcgaggcc
     2341 tgccgcgaga tcgcccgccg tcacgaggtg ctgcggacca ccttctcctc ggtggccggc
     2401 cggccggtcc aggtcattgc cgccgagccg cctccgggct ttccactgat cgacctcggc
     2461 gggctcacgg aagcggcgcg gcggaaggaa ctggcccgcc tcgccctcgc cgaggggcgg
     2521 cggccgttcg atctgacccg
//
LOCUS       DASPJI010000042        18978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_60176, whole genome shotgun sequence.
ACCESSION   DASPJI010000042 DASPJI010000000
VERSION     DASPJI010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18978)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18978
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60176"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2452)
                     /locus_tag="VEL74_01120"
     CDS             complement(<1..2452)
                     /locus_tag="VEL74_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HYG61155.1"
                     /translation="MRRAALPALSLLLFLAVPALAQTPSAPPPGQPPSPEAFLGHRVG
                     EDRRLASYPRVLEYLRVLDAASDRISIESAGRSTLDNEMAVVVLTSEANQRNLGRYRE
                     IARRLAHPEGLSTEEARSLAAEGKTIALVTCTIHSTEVGSTQMAMEFAHDVATTTDRR
                     KLAWLDDVILLLMPSINPDGQVMVIDWYNEHLGTPYEGGAMPWLYHTYVGHDNNRDFY
                     MLTQKESQVVNDVLYHRWFPQIFLDEHQMGSTGPRMFVPPQADPLAPEVSSLIFRQAD
                     LLGTVMSMRLEEAGKLGVGHDMIFDSYWPGGTRNTAWWKNITGLLTEVASARIATPIL
                     IEPGELRGGVKGLPEYTRRSNFPSPWPGGWWRLRDIVEYELVATWAFVESGSRYRDSL
                     LGNVYKMASEATERGRTEPPYAFIVPEDQHDPVAAALLVDLMLRHGVRVQRTEAPLTV
                     AVTSPGGGGRSRAVYPAGSYVIPAAQPYRQFLLTMLRPQRYPEVIPYAGGPVIPPYDV
                     TSWSLPISMGIEVAEAVEPVVNLADITAPVWPGGEVSRSAGGYLIPRGADSAVTLLNR
                     LLADGRKVFWLRQPIEGVAGSAPGDLYLPGPQLAPEELGRLSRELRVPVVPVPRAPSG
                     AAWRVEPVRVGLYKPWVASMDEGWTRFLLERYRFPHENLNNAQMKDGSYRGKVDVVLL
                     PSVDPDIIERGEPGSEEARRFQSPLPPPYAGGLGPDGGRKLKEWVEGGGTLVALDASA
                     GYAIKLLGLPVRNVLERVSEESFHCPGSMLRVRFDPEHPLSHGLQAEEAAYFADSPVF
                     QTSPPDSRIERRVVATYPN"
     gene            2656..3339
                     /locus_tag="VEL74_01125"
     CDS             2656..3339
                     /locus_tag="VEL74_01125"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HYG61156.1"
                     /translation="MNTFQKILLTGALSALLAAAPVAAQAEKRNEEFRYRWQLKNFVG
                     AVAGLFLPNQGEGSLTFTRNNGHLTSELLITSKQSKQGEFFRYGSEIDTRTLQPIRAW
                     SAYQWRGETKSKSDPIDREGVLDVASGIYSIRQDPPAKPRRMEIWSEGKIYPVVVIPL
                     GVEKRKLPSGKKVEARHYSIRGVDLKDRRKWKGKLDLWLANDEAATPVEILISRNLAD
                     VRLELMSAP"
     gene            3429..4070
                     /locus_tag="VEL74_01130"
     CDS             3429..4070
                     /locus_tag="VEL74_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HYG61157.1"
                     /translation="MSRCKIVWMIPLAALLLALPACRREQTETPDSAVLENTAAPEGT
                     VPPLGAPVDPTGPTARATLQGAPEDTDFTGTVTFTQQGDGLIVIADFAGVDTPGEHGF
                     HVHETGDCSHDAAGKHFTSAGGHFNPNNVEHACPPNEPRHAGDLGNVQIGTNGAGRSE
                     QTTNLLTLSGPNSVVGKAIILHTGADDCQTQPTGNAGDRLACGVVTMEGSPAQ"
     gene            4067..5287
                     /locus_tag="VEL74_01135"
     CDS             4067..5287
                     /locus_tag="VEL74_01135"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYG61158.1"
                     /translation="MSRRLRPAVVLAAVLALPGISGGAGPVRPAAGWKPLDPPAVAGS
                     LSPNLSLAGGEVTLTWLEPLVDPKGRQTHRLRVSRLSGGRWSAPATAAAGPDFFANWA
                     DFPGLIQSPDGALLVFWLARTTGGGDYDYAVHLARSTDGGRTWKPAGVLHDDGIPAEH
                     GFVAWVPEGKSVRAFWLDGRETKSGGAMTLRTATVTDKAGASERLDARICDCCQTDAA
                     VAADGPVVVYRDRSDDEIRDISIIRRTAAGWSRPARVHADEWKIPGCPVNGPAVAAAG
                     RKVAVAWFTGAPPGAQVQVAFSEDGGARFGAPVRIDAGKPLGRVDVALAPDGSAVVSW
                     LELLEGGRAAVRLRRVRNGKAGEPVEIARTGSARASGFPRLLLAGDRLHLSWVEDGDA
                     ARLHAGSLPLASIP"
     gene            5504..6985
                     /locus_tag="VEL74_01140"
     CDS             5504..6985
                     /locus_tag="VEL74_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HYG61159.1"
                     /translation="MVLALSGPALASSHREAPAITELPKVDNTDIYMFNSYEAGRTGF
                     VTLIANFQPFQDPFGGPNYFTMDPDARYRFHIDNTGDGVEDITFQFRVTQLLRDLQVP
                     VGGKNVSVPLINIGPITGSPQNRSPNLNVLESYTLSVIRGRVDAGNRNVEFARNMDAG
                     TSRFDKPVDNIGQKSIPNYAAYSRNFIWNFTVPGCGTSQGRVFVGQRKEPFYINVGEI
                     FDLVNIEDPIGDRDTEPNALDDKNITTFALEVPANCLRGSGPVIGGWATAALPRNRGL
                     NSNPEFKDPYNETGDFVQVSRLGMPLVNEVVIGIKDKNRFNASPPRMDAQFLDYVTNP
                     TLPELLEILFGAAGVRAPNNFPRTDLVAAFLTGLPGLNQFGSPAEMMRLNTNIPAVPA
                     AQQNNLGALGGDNAGFPNGRRPGDDVVDIELRVAMGVLCHAFPGAFCNPADAPSGNLP
                     FTDGTLQDASQFDGFFPYLRAPLPGSPNGPNGVDENDGDGDGD"
     gene            7060..7872
                     /locus_tag="VEL74_01145"
     CDS             7060..7872
                     /locus_tag="VEL74_01145"
                     /inference="COORDINATES: protein motif:HMM:NF022135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HYG61160.1"
                     /translation="MISRLCVLLLVTALWAAPLLAHDFWIEPASFHPAPGERVAVRLR
                     VGEGFQGEPVPRMSGRIERFVTVGAPGETPLQGVEGMDPAGWATFQSPGLYQVIYDSH
                     HARVELDGPAFEKYLAEEGLESVIAQRAAKGQSAARAREVYSRSAKSLLAVGDGAAGS
                     GWDRAAGLELELVPEANPYKLTPLAPDTELPVRLLFRGQPLSGALIVAFPKSDPTARV
                     SARSDAQGRVRLRLGQPGVWLVKAVHMIPAAAGADADWESQWASLTFHLPTK"
     gene            8000..8530
                     /locus_tag="VEL74_01150"
     CDS             8000..8530
                     /locus_tag="VEL74_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG61161.1"
                     /translation="MSRMAAGDGDAFARLFDRHSPVALGLLHRILGGRAEAEEVLQEV
                     FLQAWTQGSRYDGERATPRGWILMLARSRALDRLRRRDAARRRDEEAGDEQARLGISS
                     PVGTERLEARERRDRVHSALGQLSPEQRRCIELAFFDGLTHTQIAERLEAPLGTVKSR
                     ILLGMNKLRQALRSAS"
     gene            8527..9345
                     /locus_tag="VEL74_01155"
     CDS             8527..9345
                     /locus_tag="VEL74_01155"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HYG61162.1"
                     /translation="MSAGHTPRFEEMLPAYALGALEGDELHELEAHLATGCDECRRQL
                     RMWEADLEEIAASVSPMTPSAETRQRVLEATAPATQPAPARLEPRRTRSAGRRGRWGW
                     LAAAAALALVVWSQWNVARLRQETADLAQERDRLARQVTSLQSERDLARAEAQRMAQA
                     LGFITSPGVRSIQLAGLEAAPGAAGHTFVDPRRRQAVFYASGLPALAPDQTYQLWFIT
                     DRPVSAGTFSVDGSGRGNLQVDGVADGVRTWAVTVEPSGGAPQPTGAMVLAGQA"
     gene            9389..10423
                     /gene="pta"
                     /locus_tag="VEL74_01160"
     CDS             9389..10423
                     /gene="pta"
                     /locus_tag="VEL74_01160"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006806879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HYG61163.1"
                     /translation="MGEGELLLTSSSPTDQPLDPLARLHARARGTGASVFLAEGEDPR
                     VVAAAERAAAEDVCRPSLVGPRSVVEQAAAEAGVELTVPVLDPALDPDLAHHAAVLTA
                     RLAPRFTPEEAAVFVRDPLYYAALQVAAGRADGAVMGARATTADTLRAALRVIGPRPG
                     LKVVSSCFLMVMPDGRGLIYSDCGVVPDPGPDELADIAESAAASCRFLLGEEPRVALL
                     SFSTRGSAKHPHVDKVRSALERLRARGVSFAVDGELQADAALVPDVARRKAPDSPVAG
                     QANVLVFPDLDAGNIAYKLTERLAGARAVGPLLQGLARPIHDLSRGCNVQDIVDAMAV
                     AAVQAQEKPA"
     gene            10420..11721
                     /gene="purD"
                     /locus_tag="VEL74_01165"
     CDS             10420..11721
                     /gene="purD"
                     /locus_tag="VEL74_01165"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HYG61164.1"
                     /translation="MRVLVVGGGGREHALCWKLAQSPLLTRLWCAPGNPGIAQVAATV
                     DIAPEDINRLVDFAVAQRVDLTVVGPEVPLTLGLADALAARGLAVFGPTRAAAELEGS
                     KVFAKRFLERHGIPSAPFAVVADAAAAREAMGRFGWPVVLKADGLAAGKGVLIAQDEA
                     EYEDAVRALFEERRFGASADQVVIEGFLHGEEVSFMALSDGERLLPLATSKDYKRIGD
                     GDTGPNTGGMGCHSPSGLVTAGAAERIAETILRPVIAGLAAEGRPFTGILYAGLMLTE
                     DGPRVLEFNARFGDPEAQVLLLRLEDDLLPVLAAGAAGRFEVPELRFRSEAAACVVLA
                     SPGYPGKPRQGEPIRGLGRAGSPPGVEIFHAGTALVDAESDTELVSAGGRVLSVCALG
                     ADLAAALERAYAAVTTIDWPGKVFRRDIGARVLARPWKGGT"
     gene            11918..12580
                     /gene="ricT"
                     /locus_tag="VEL74_01170"
                     /pseudo
     CDS             11918..12580
                     /gene="ricT"
                     /locus_tag="VEL74_01170"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
     gene            12683..12997
                     /locus_tag="VEL74_01175"
     CDS             12683..12997
                     /locus_tag="VEL74_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61165.1"
                     /translation="MAELKKELPARSVRRKQSPPEQTNAEEFYYLKQMAAKTPMVVIL
                     TDGEELRGWIEWYDKGALKLNRTSGPNLLIQKHVIRYMFKEEELKQRGRRRVSNDESS
                     ED"
     gene            12994..14055
                     /locus_tag="VEL74_01180"
     CDS             12994..14055
                     /locus_tag="VEL74_01180"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HYG61166.1"
                     /translation="MTRTVPRLALTQGDPAGIGPEILLKLLSEKETGPVHEDARGDTP
                     GWQPLLVAERAALEALRPSLPHAPWDRLRYLDAPPGPEEALFGDGEIPVLDPVATRRA
                     ITLGDSGPADAFGAMAALDAGIALVRSGAADALVTAPVSKSSIARQHLPGFKGHTDYL
                     AEACGLERYGRDYLMAFLAPELQVALLTVHVPMREALDAVEPVTVTEALECLHRHAGG
                     KIALAGLNPHAGEGGLLGEEEGRLLVPTVAAARERGIDVHGPESADSLFARARRGEFD
                     WVLALYHDQGLIAVKTAAFGLATNWTLGLPFLRTSVDHGTAFPLAGKGIADAGPMEAV
                     IETTLELISGTLPRKRTTE"
     gene            complement(14449..14742)
                     /locus_tag="VEL74_01185"
     CDS             complement(14449..14742)
                     /locus_tag="VEL74_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prevent-host-death protein"
                     /protein_id="HYG61167.1"
                     /translation="MRAVARRALEDQLDEFLHLVQEGETILVTEHDRVIAELVPPRES
                     GDLPAMNPALLDGVRKGWITPPALAKEPAPPLPPPVAPLEDILRELDEDRADR"
     gene            complement(14842..15387)
                     /locus_tag="VEL74_01190"
     CDS             complement(14842..15387)
                     /locus_tag="VEL74_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61168.1"
                     /translation="MRSFDERSTSSIHPNSFTTQFLGRMAERDEPPTAGEADVAGPWR
                     VEPVAWNGGSGYGLFRIGESLARGFAPVGLYPSRWLALVAAAVLPGTGRDPLIRLEKQ
                     PDADGVYPLVLLPGADGGEVVGHLQLFDEALIDAMNVAIHLLQAPESLAWLLEAAGSV
                     TLERCGAILDERVAAGVEGEG"
     gene            15817..16194
                     /locus_tag="VEL74_01195"
     CDS             15817..16194
                     /locus_tag="VEL74_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61169.1"
                     /translation="MATRRETTKTGILGTWRELLPPLMANAAELPQLEIPRGKLERIL
                     GRAQEVVHLQGLLAANRQELTREWDLLQAEGERVAALLRRGIKEHYGPRAEKLAEFGL
                     QPFRGRKAKKVTEAEEPDTPTLS"
     gene            complement(16979..17401)
                     /locus_tag="VEL74_01200"
     CDS             complement(16979..17401)
                     /locus_tag="VEL74_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61170.1"
                     /translation="MRPSRVVFAALFTFAAWAASASASTYANFSGNCIRFDSSSPAYC
                     VFDGNRTYDNGHTSDTTATTCGNASVSAVYWDFGDGTGTWDDLYVGHTYSDPAAIDND
                     AIITMTLFCSDNTWDEKVRYLLFVLIGPGDMYCNEGWN"
     gene            17754..18197
                     /locus_tag="VEL74_01205"
     CDS             17754..18197
                     /locus_tag="VEL74_01205"
                     /inference="COORDINATES: protein motif:HMM:NF023695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HYG61171.1"
                     /translation="MPAEEPGALNIEGLAAKDGKLLIGFRGPVRGGKALVVTLENPQE
                     VLNQGAPASFATPIELDLDGLGIRSLEHWPAEARRSCTATSLSPVQGLRVEPKAAYEA
                     MSTPRTDGAAGGRRLASSILPASQEVATAYLLMWTCWCSFGLLGG"
     gene            complement(18146..18559)
                     /locus_tag="VEL74_01210"
     CDS             complement(18146..18559)
                     /locus_tag="VEL74_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61172.1"
                     /translation="MGTVFALENLTQEDLSLDYVMAYDGKSWQFVVQKPDASRINNDI
                     NPGFYLISLSSFTIPKGGSISVDTIFNTWLLYKAGLASDNAFLLFSIKGSNLHVGLNG
                     EYLRNTGVYGSNPGAVKWSYPPSNPNEHQHVHINR"
     gene            complement(18755..>18978)
                     /locus_tag="VEL74_01215"
     CDS             complement(18755..>18978)
                     /locus_tag="VEL74_01215"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017726499.1"
                     /note="PEP carboxykinase; PEP carboxylase; PEPCK;
                     catalyzes the phosphorylation and decarboxylation of
                     oxaloacetate to form phosphoenolpyruvate using ATP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HYG61173.1"
                     /translation="ETWTDPIFGLNVPVHVEGVPDNVLRPRETWQNPADYDAKASQLA
                     DMFADNFEKYEAGVSEEIKAAGPRKAVAR"
BASE COUNT         2946 a         6792 c         6416 g         2824 t
ORIGIN      
        1 cgttcgggta ggtggcgacc acccggcgct cgatgcggct gtccggcgga ctggtctgga
       61 agaccggcga gtcggcgaag taggccgcct cctccgcctg gaggccgtgg ctcagcgggt
      121 gctccgggtc gaaccggacg cgcagcatgg agccggggca gtggaagctc tcctcgctca
      181 cccgctcgag gacgttgcgc accggcaggc cgaggagctt gatggcgtac cccgccgagg
      241 cgtccagggc gaccagggtg ccgccccctt ccacccactc cttgagcttg cggccgccgt
      301 ccggcccgag ccccccggcg taggggggcg gcagcgggga ctggaagcgc cgcgcctcct
      361 ccgagccggg ctcgccgcgc tcgatgatgt ccgggtcgac cgagggcagg agcaccacgt
      421 ccaccttgcc gcgataggag ccgtccttca tctgggcgtt gttcaggttc tcgtgcggga
      481 accggtagcg ctccaggagg aaccgggtcc acccctcgtc catgctcgcc acccacggct
      541 tgtagaggcc gacgcgcacc ggctcgaccc gccacgccgc gcccgacggg gcccgcggca
      601 ccggcaccac cggcacccgg agctcccgcg acaggcgccc cagctcctcg ggggcgagct
      661 ggggaccggg caggtagaga tcgcccgggg cgctgccggc caccccctcg atcggctggc
      721 gcagccagaa gaccttgcgg ccgtcggcga gcagccggtt caggagggtg acggcggagt
      781 cggcgccgcg ggggatcagg tagccgccgg cgctccggga cacctcgccg cccggccaga
      841 ccggcgccgt gatgtccgcg agattcacca ccggctccac cgcctcggcc acctcgatcc
      901 ccatggagat cggcagcgac caggaggtga catcgtacgg cgggatcacc ggcccgccgg
      961 cgtaggggat cacctcgggg tagcgctgcg ggcggagcat ggtgagcagg aactggcggt
     1021 agggctgcgc ggccgggatc acgtaggagc ccgcggggta gactgcgcgg ctccgccccc
     1081 cgccccccgg gctcgtgacc gcgacggtga gcggcgcctc ggtccgctgc acccgcacgc
     1141 cgtgccgcag catcaggtcc acgaggaggg ccgcggccac cgggtcgtgc tggtcctccg
     1201 gcacgatgaa cgcgtacggc ggctcggtcc gcccgcgctc cgtggcctcg ctcgccatct
     1261 tgtagacatt gccgagcagg ctgtcgcggt agcgcgaccc cgactcgacg aaggcccagg
     1321 tggcgaccag ctcgtactcg acgatgtcgc gcagccgcca ccagcccccc ggccaggggg
     1381 aagggaagtt ggagcggcgg gtgtactcgg gcagcccctt gacgccgccg cgcagctcgc
     1441 cgggctcgat gaggatgggg gtggcgatgc gggcgctcgc cacctcggtg agcaggccgg
     1501 tgatgttctt ccaccacgcg gtgttgcggg tgccgcccgg ccagtagctg tcgaagatca
     1561 tgtcgtgccc gacgccgagc ttgcccgcct cctccagccg catgctcatc accgtgccca
     1621 ggaggtcggc ctgccggaag atcagcgagc tcacctcggg ggcgagcggg tcggcctgcg
     1681 gcggcacgaa catgcgcggg ccggtggagc ccatctggtg ctcgtccagg aagatctgcg
     1741 ggaaccagcg gtgatagagg acgtcgttga cgacctggct ctccttctgg gtgagcatgt
     1801 agaagtcgcg gttgttgtcg tgcccgacgt aggtgtggta gagccagggc atggcccccc
     1861 cctcgtacgg ggtgccgagg tgctcgttgt accagtcgat gaccatcacc tggccgtccg
     1921 ggttgatcga cggcatgagg agcaggatga cgtcgtcgag ccaggcgagc ttccgccggt
     1981 cggtggtggt ggccacgtcg tgggcgaatt ccatcgccat ctgggtcgat cccacctcgg
     2041 tggagtggat cgtgcaggtg accagggcga tggtcttgcc ctcggcggca agggaccggg
     2101 cctcctcggt cgacagcccc tccggatggg cgaggcgccg cgcgatctcg cggtagcggc
     2161 cgaggttgcg ctggttggcc tccgaggtga gcaccaccac ggccatctcg ttgtccaggg
     2221 tggagcgccc ggcgctctcg atgctgatcc ggtcggacgc ggcgtcgagg acgcgcaggt
     2281 actccagcac cctgggatag gaagccaggc gccggtcctc cccgacccgg tggccgagga
     2341 acgcctccgg cgacgggggc tggcccggcg gcggggcaga gggggtctga gccagcgccg
     2401 gaacggcgag gaacaggaga agggagaggg ccggcagggc ggcacggcgc atggtgtcct
     2461 ccttgtagga acggggcccg cggggaccga ccgcgagtct acccgagaaa gtatgaaaat
     2521 ccgggcatgg cagcgccctt gctattgtta tagagggggc acccccggcg gcggcctcca
     2581 tgggtggagc gatcctgctc caggctcctg acaccctgac gggctccccg ggataaactc
     2641 cgagagagaa gcgcgatgaa cacctttcag aagatcctgc tgaccggcgc cctgtccgcc
     2701 ctgctcgccg ccgccccggt ggcggcgcag gccgagaagc gcaacgagga gttccgctac
     2761 cgctggcagc tcaagaactt cgtcggcgcg gtggccgggc tcttcctgcc caaccagggc
     2821 gaggggtccc tcaccttcac ccggaacaac ggccatctga cgagcgagct gctgatcacc
     2881 tccaagcaga gcaagcaggg ggagttcttc cggtacggct cggagatcga cacgcggacg
     2941 ctgcagccga tccgagcctg gagcgcgtat cagtggcgtg gcgagaccaa gtcgaagagc
     3001 gaccccatcg accgcgaagg ggtgctcgat gtcgcctccg gcatctactc gatccgccag
     3061 gacccgccgg ccaagccgcg gcgcatggaa atctggtccg agggcaagat ctacccggtg
     3121 gtggtgatcc ccctgggggt cgagaagcgc aagctgccca gcggcaagaa ggtggaggcc
     3181 cgccactact cgatccgcgg cgtcgacctg aaggaccgcc gcaagtggaa gggcaagctg
     3241 gacctctggc tcgccaacga cgaggcggcc acgccggtgg agatcctgat ctcccgcaac
     3301 ctggcggacg tccgcctgga gctgatgtcc gcaccctgag ccctcgggcc ctcgccgaca
     3361 gccggagcca gccggccgcg gcccgggaga accccgcgat cttttcgacg aaagaggagg
     3421 agagtgtcat gagccgttgc aagatcgtct ggatgattcc gctggcggcg ctgctgctgg
     3481 ccctgcccgc ctgccgccgc gagcagaccg aaacccccga ttccgccgtt ctggagaaca
     3541 ccgccgcccc cgaggggacg gtgccgccgc tgggagcccc cgtggacccc accggaccca
     3601 ccgcccgggc gactctgcag ggagctcccg aggacaccga cttcaccggc accgtgacct
     3661 tcacccagca gggtgacggc ctgatcgtga tcgccgactt cgccggcgtg gacaccccgg
     3721 gtgagcacgg cttccacgtc cacgagaccg gcgactgctc ccacgacgcg gccggcaagc
     3781 acttcacctc cgccggcggc cacttcaacc ctaacaacgt ggagcacgcc tgcccgccca
     3841 acgagccgcg ccacgccggc gacctgggca acgtccagat cggcaccaac ggcgccggcc
     3901 ggtcggagca gacgacgaac ctgctcaccc tctccggccc caactcggtg gtcggcaagg
     3961 ccatcatcct ccacaccggc gccgatgatt gccagacgca gcccaccggc aacgccggcg
     4021 accgcctcgc ctgcggcgtg gtgacgatgg aaggcagccc ggcccagtga gccggcggct
     4081 ccggcccgcg gtggtcctcg cggcggtcct ggcccttccc ggaatctcgg gcggggccgg
     4141 tcccgtccgg cccgccgccg gctggaagcc tctcgacccg cccgcggtgg cgggctccct
     4201 gtcccccaat ctctccctgg ccgggggcga agtgacgctc acctggctgg agccgctggt
     4261 ggacccgaag ggccgccaga cccaccgcct gcgggtctcc cgtctttcag gaggtcgctg
     4321 gtcggctccg gccaccgccg ccgccggccc cgacttcttc gccaactggg ccgacttccc
     4381 cggcctgatc cagtcgcccg acggggccct gctggtcttc tggctcgcca ggaccaccgg
     4441 cggcggcgac tacgactacg ccgtccatct cgcgcgctcc accgacggcg gccgtacctg
     4501 gaagccggcc ggcgtgctgc acgacgacgg catccccgcc gagcacggct tcgtcgcctg
     4561 ggtgcccgag gggaagagcg tgcgcgcctt ctggctcgac ggccgcgaga ccaagagcgg
     4621 cggcgccatg accctgcgca ccgccacggt caccgacaag gccggcgcca gcgagcggct
     4681 cgacgcccgc atctgcgact gctgccagac cgacgccgcc gtcgctgccg acggcccggt
     4741 ggtggtctat cgcgaccgct ccgacgacga gatccgcgac atctcgatca tccgccgcac
     4801 cgccgcgggc tggagccggc cggcgcgcgt ccacgccgac gagtggaaga tccccggctg
     4861 cccggtcaac ggcccggccg tggccgccgc cggccgcaag gttgccgtcg cctggttcac
     4921 cggcgcgccc cccggtgccc aggtccaggt ggccttctcg gaagacggcg gcgcccgctt
     4981 cggcgctccg gtgcggatcg acgccggcaa gccgctcggc cgggtcgacg tcgccctggc
     5041 gcccgacggc agcgccgtgg tgagctggct ggagctcctg gaggggggcc gcgccgccgt
     5101 ccgcctccgc cgggtacgca atgggaaggc cggagagccg gtggagatcg cccgcaccgg
     5161 ctccgcccgc gccagcggct tcccgcgcct gctcctcgcc ggcgaccgcc tccatctgtc
     5221 ctgggtggag gatggcgacg cggcgcgcct ccacgccggc agcctgccgc tggcgtcgat
     5281 cccgtaaatc tttcttttcg actcatccaa cccgtcaggg tctccgtatc cccttcagag
     5341 acgaggccgg ggtccatcat ccccggcgcc ggcgacggcg gtccggttcc acaacagcag
     5401 tcgcaacggc cgtcccggaa gctttcaccc cctcgatccg aggagaaaca aggaggagat
     5461 gatgaggaga agtgtgcgtg tcgcgggtct tctgactctg gccatggtgc tcgccctgtc
     5521 gggtccggcc ctggcttcca gccaccggga ggcgccggcg atcaccgagc tgcccaaggt
     5581 ggacaacacc gacatctaca tgttcaacag ctacgaggcg gggcgtaccg gattcgtgac
     5641 gctgatcgcc aacttccagc cgttccagga tcccttcggc gggcccaact acttcaccat
     5701 ggatccggac gcccggtacc gcttccacat cgacaacacc ggtgacgggg tcgaggacat
     5761 caccttccag ttccgcgtca cccagctcct gcgcgacctc caggtaccgg tgggcggcaa
     5821 gaacgtctcg gtgccgttga tcaacatcgg gccgatcacc ggctctcccc agaaccgctc
     5881 gcccaacctg aacgtcctcg agagctacac gctctccgta atccgcggcc gcgtcgacgc
     5941 cggcaaccgc aatgtggagt tcgcccgcaa catggacgcc ggcacctccc gcttcgacaa
     6001 gccggtggac aacatcggcc agaagtccat ccccaactac gccgcctatt ccaggaactt
     6061 catctggaac ttcacggttc cgggctgcgg cacctcacag ggccgggtct tcgtcggcca
     6121 acgcaaggag ccgttctata tcaacgtcgg cgagatcttc gacctggtca acatcgagga
     6181 cccgatcggc gaccgtgaca ccgagcccaa cgctctcgat gacaagaaca tcaccacctt
     6241 cgccctcgag gtgccggcca actgcctgcg cggcagcggc ccggtgatcg gcggctgggc
     6301 caccgccgcc ctgccgcgca accgcggact caacagcaat cccgagttca aggatcccta
     6361 caacgagacc ggcgatttcg tacaggtctc gcggctcggc atgccgctgg tcaacgaagt
     6421 cgtaatcggc atcaaggaca agaaccgctt caatgccagc ccgccgcgca tggacgcgca
     6481 gttcctcgac tacgtcacca acccgacgct gcccgagctg ctggaaatcc tgttcggcgc
     6541 cgccggcgtg cgggccccga acaacttccc gcgcaccgat ctggtcgccg ccttcctgac
     6601 cggcctcccg ggcctcaatc agttcggctc gcccgccgag atgatgcgcc tgaacaccaa
     6661 catcccggcc gtcccggccg cgcagcagaa caacctcggc gcgctgggcg gcgacaacgc
     6721 cggcttcccc aacggccggc gccccggtga cgacgtggtg gacatcgagc tgcgggtggc
     6781 gatgggcgtc ctttgccacg ccttcccggg cgccttctgc aacccggccg acgccccgtc
     6841 gggcaacctg ccgttcaccg acggcacgct ccaggatgcc agccagttcg acggcttctt
     6901 cccctatctc cgcgcgccgc tgcccggctc gccgaacggc cccaacggcg tcgacgagaa
     6961 cgacggcgac ggcgacggcg actaagccga ggccacgctc cggggcggcc gaatgcatca
     7021 ggcatggccg ccccggtccc ttcgatccgg gagaatccca tgatctctag actttgtgtc
     7081 ctcctgctgg tcacggccct atgggcggcg cccctcctcg cccacgactt ctggatcgag
     7141 cccgccagct tccaccccgc tccgggcgag cgcgtcgccg tgcggctgcg ggtcggcgag
     7201 gggttccagg gggagcccgt gccgcggatg agcggccgga tcgagcgttt cgtgacggtc
     7261 ggtgccccag gggagacgcc tctccaggga gtggaaggaa tggacccggc gggctgggcg
     7321 accttccaga gccccggcct ctatcaggtg atctacgaca gccaccacgc ccgcgtcgag
     7381 ctcgacggcc cggccttcga aaagtacctc gccgaggagg ggctggaatc ggtcatcgcc
     7441 cagcgcgccg ccaaggggca gagcgccgcc cgcgcccgtg aggtgtactc gcgctccgcc
     7501 aagtcgctgc tcgcggtggg ggacggcgcc gccggctccg gctgggaccg ggcggccggc
     7561 ttggagctgg agctggtgcc ggaggccaac ccctacaaat tgacgcccct ggcacctgac
     7621 accgagctgc cggtccgcct cctcttccgc ggccagccgc tgagcggggc cttgatcgtc
     7681 gcgttcccca aatccgaccc cacggcccgg gtgtcggcgc gtagcgacgc ccaggggagg
     7741 gtccggctgc gtcttggcca acccggggtc tggctggtca aggcggtcca tatgatcccc
     7801 gcggcggcgg gggctgatgc cgactgggag agccagtggg catccctgac gttccacctc
     7861 cccacgaaat aaggcatcct tcgccggcag cgcgggcacg cagattgaat agactcgggc
     7921 tgcccggggc gcctgaagtc ttttcgcacc ccctgccaac tcctagatgg acccctggga
     7981 agccgaagat cgagatctca tgagccggat ggccgccgga gacggcgatg ccttcgcgcg
     8041 ccttttcgac cggcactcgc cggtcgccct ggggctgctc caccgcatcc tcggtggccg
     8101 ggcggaggcc gaagaggtcc tgcaggaggt cttcctccag gcctggacgc aggggagccg
     8161 ctacgacggc gagcgcgcca ccccgcgcgg ctggatcctg atgctcgccc gctcccgcgc
     8221 cctcgaccgg ctgcgccgcc gcgacgccgc ccgccgccgt gacgaggagg ccggcgacga
     8281 gcaggcgcgt ctcggaatct ccagcccggt gggcacggag cggctggagg cccgcgagcg
     8341 gcgcgatcgg gtccattcgg cgctggggca gctctcgccc gagcagcgcc gctgcatcga
     8401 gctcgccttc ttcgacggtc tcacccacac ccagatcgcc gagcggctgg aggctcccct
     8461 cggcacggtc aagtcccgta tcctgctcgg aatgaacaag ctccggcagg ccctgaggtc
     8521 cgcgtcatga gcgccggcca cacgccccgc ttcgaggaga tgctccccgc ctacgccctg
     8581 ggcgcgctgg agggggacga gctccacgag ctggaggccc acctcgccac cggctgcgac
     8641 gagtgccggc ggcagctccg gatgtgggaa gccgacctgg aggagatcgc ggcctcggtc
     8701 tctcccatga cgccctcggc cgagacgcgg cagcgcgtgc tcgaggctac agcgccggca
     8761 acccagccgg cgcccgcgcg cctcgagccc cgccgtaccc ggtccgctgg ccgccgaggc
     8821 cgctggggct ggctcgccgc ggccgccgcc ctcgccctgg tggtgtggag ccagtggaac
     8881 gtcgcgcggc tgcggcagga gacggcggac ctcgcccagg aacgcgaccg cctggcgcgg
     8941 caggtgacct ccctccagag cgagcgcgac ctcgcccgcg ccgaagcgca gcgcatggcc
     9001 caggcgctcg gcttcatcac ctctcccggc gtgcgctcca tccagctcgc cggcctcgaa
     9061 gccgcgcccg gcgccgccgg ccacaccttc gtcgatccgc ggcggcgcca ggccgtcttc
     9121 tatgcctccg gcctgccggc gctcgccccg gaccagacct accagctctg gttcatcacc
     9181 gaccggccgg tctccgccgg caccttctcc gtcgacggct ccggccgcgg caacctgcag
     9241 gtggacggcg tcgccgacgg ggtgcgcact tgggcggtca ccgtcgagcc cagcggcggc
     9301 gcgccgcagc ccaccggcgc catggtcctg gcgggccagg cctgacctct ctgccttttg
     9361 ttatagaatc tccggtccaa gatccaccgt gggcgagggt gagcttcttc tgacttcttc
     9421 atctccaacc gaccagcctc tcgatcccct ggcgcgcctg cacgcccggg cccgcggcac
     9481 gggcgcttcg gttttcctcg ccgagggcga ggacccgcgc gtcgtggccg ccgccgagcg
     9541 cgccgccgcc gaggacgtct gccggccgag cctcgtcggc ccgcggagcg tcgtggagca
     9601 ggccgccgcc gaggcgggcg tcgagctgac cgtgccggtc ctcgacccgg cgctcgatcc
     9661 cgacctggcg catcacgccg ccgtgctcac ggcccgcctc gcgccgcgct tcacccccga
     9721 ggaggccgcc gtcttcgtcc gcgacccgct ctactacgcg gcgctacagg tggccgccgg
     9781 ccgcgccgac ggcgccgtca tgggggcgcg cgccaccacc gccgacacgc tgcgcgcggc
     9841 gttgcgggtg atcggcccgc ggccgggcct caaggtggtc tcctcctgct tcctcatggt
     9901 gatgcccgac ggccgcggcc tgatctacag cgactgcggc gtcgtgcccg atcccggccc
     9961 cgacgagctg gcggacatcg ccgagtcggc ggcggcgagc tgccgcttcc tgctcggcga
    10021 agagccccgg gtggccctcc tctccttctc cacccgcggc agcgccaagc atccgcatgt
    10081 ggacaaggtg cggtcggcgc tggagcggct gcgcgcccgc ggcgtctcct tcgccgtcga
    10141 cggcgagctc caggccgacg ccgctctggt gccggacgtg gcccgccgca aggcgccgga
    10201 cagcccggtg gccgggcagg ccaacgtcct cgtctttcct gacctcgacg ccggcaacat
    10261 cgcctacaag ctcaccgagc ggctggccgg cgcgcgcgcc gtgggccccc tcctccaggg
    10321 gctcgccagg ccgatccatg acctgtcgcg cggctgcaac gtccaggaca tcgtcgacgc
    10381 catggccgtc gccgccgtcc aggcacagga gaaaccggca tgagggtgct ggtcgtcggc
    10441 ggcggcggac gcgagcacgc cctctgctgg aagctcgccc agagccccct cctcacccgc
    10501 ctctggtgcg cgccgggcaa tccggggatc gcccaggtcg ccgcgaccgt ggacatcgcc
    10561 cccgaggaca tcaaccgcct ggtggacttc gcggtggcgc agcgcgtcga cctgacggtg
    10621 gtcggccccg aggtcccgct caccctgggg ctggcggacg ccctcgccgc ccgcggcctc
    10681 gccgtcttcg gcccgacccg ggcggcggcc gagctcgagg ggagcaaggt cttcgccaag
    10741 cgcttcctgg agcggcacgg catcccctcg gcgccgttcg ccgtggtggc ggacgccgcc
    10801 gcggcgcggg aggccatggg gcgcttcggc tggccggtgg tgctcaaggc cgacggcctc
    10861 gccgcgggca agggggtgct catcgcccag gacgaggcgg agtacgagga cgccgtccgc
    10921 gccctgttcg aggagcgccg cttcggcgcc agcgccgacc aggtggtgat cgaaggcttc
    10981 ctccacggcg aggaggtctc cttcatggcc ctctccgacg gcgagcgcct cctccccctg
    11041 gccacctcca aggactacaa gcggatcggc gacggcgaca ccggcccgaa caccggcggc
    11101 atgggctgcc acagcccctc cggcctggtc acggccggcg ccgccgagcg gatcgccgag
    11161 accatcctgc ggccggtgat cgccggcctc gcggccgagg ggcggccgtt cacgggaatt
    11221 ctgtacgccg gtttgatgct cacagaggat gggcctcgcg tcctcgagtt caacgcccgc
    11281 ttcggcgacc ccgaggcgca ggtcctgctc ctccggctcg aggacgacct gctgccggtg
    11341 ctcgccgcgg gcgcggccgg ccggttcgag gtcccggagc tgcgcttccg ttccgaagcg
    11401 gcggcctgcg tcgtgctggc gagccccggc tatccgggca agccgcggca gggggagccg
    11461 atccggggac tgggccgggc cggatcgccg ccgggggtcg agatcttcca cgccggcacg
    11521 gcgctggtgg atgccgaatc ggacaccgag ttggtgagcg cgggcgggcg ggtgctctcg
    11581 gtctgcgccc tgggcgccga cctggcggcg gcgctcgagc gggcctacgc cgccgtgacg
    11641 acgatcgact ggccgggcaa ggtcttccgc cgcgacatcg gcgcgcgggt gctggcccgg
    11701 ccttggaagg gtgggacgtg agcgggcgcc ggcgagcggt tcgccggcgc tgaaggggaa
    11761 ctgctgtaac atggcttacg ccgttccgta cccaaatttc cgccggtccc tgatgggccg
    11821 gtgttctctc gcgcggctgg tacctatcgt gcataggact ctggtcgctg tcgtatagga
    11881 agtcaccatg ggatgcagtg gctgtgcttt caagggcatg tcgacagccg tcgaagactc
    11941 cttcgtcggg gtgcggttcc gggaggagac gaatctgact ctctgctcta ccaggggcag
    12001 gatctacgcg cgaggtgagg cggtggtggt ggacctggag tccggcccgg ctttcgggcg
    12061 ggtcgagcaa tctccgatgc cggtgttcaa gccgtgccag aaatcgagcg cccgtccgat
    12121 cctgcgggcc gccgacgagg cggacacggc ggcgttcgac cgccagcggt cgagcgagat
    12181 ggccggcaag ctcttcgcgc aggagcgggc ccgcgccctg agcctcgaca tgaagatctc
    12241 cctggtggac ttctcgttca gcgggaagaa agccactttt tactttacct ctgacgggcg
    12301 ggtggacttc cggcaactgg tgcgggacct ctcctcgcgc ttctcggtgc gggtgaagat
    12361 ggtccaggtc ggggcgcgcg atgaggcggg cctcctgggc ggcatcggca tctgcggccg
    12421 caccctctgc tgctcgacct ggctcaagga cttccggccc atctccatcc agatggccaa
    12481 gcggcagaac ctgagcctca atccgtcgaa gatctccggc cagtgcgggc gtctgctctg
    12541 ctgcctggcc tatgaagacg atcagtacaa gaaaggcaac ccggacaagc cgggccccgg
    12601 ccccatcgcc ggcggctcgg aggccacgta ttagaggcgc ctggaatcca caatggccaa
    12661 tcgcaaactc attcgccccg acatggctga gctcaagaag gagctccctg cacgtagcgt
    12721 tcgccgcaag cagtcgccgc cggagcagac caacgccgag gagttctact acctcaagca
    12781 gatggcggcc aagacgccga tggtggtcat cctgacggac ggcgaggagc tccggggctg
    12841 gattgagtgg tacgacaagg gggcgctgaa gctcaaccgc acctcgggac cgaacctgct
    12901 catccagaag cacgtgatcc gctacatgtt caaagaggaa gagctcaagc agcgggggcg
    12961 ccgccgcgtc tccaatgacg agtcctccga agattgaccc ggaccgtgcc ccgtctcgcc
    13021 ctcacccagg gtgacccggc cggcatcggt ccggagatcc tcctcaagct cctgtcggag
    13081 aaggagacag ggccggtcca cgaggacgcc cggggggaca ctccgggctg gcagccgctg
    13141 ctcgtcgccg agcgcgccgc cctggaggcg ctccgcccct ccctccccca cgctccctgg
    13201 gaccgcctgc gctacctcga cgctcccccc ggacccgagg aagccctctt cggagacggc
    13261 gagatcccgg tcctcgaccc tgtcgccacg cgccgcgcca tcaccctggg cgactcgggc
    13321 cccgccgacg ccttcggcgc catggcggcg ctggacgccg gcatcgccct ggtccgctcc
    13381 ggagccgccg acgccctggt gaccgcgccg gtcagcaagt cctccatcgc tcgccaacac
    13441 ctccccggat tcaaggggca caccgactac ctggccgagg cctgcggcct ggagcgctac
    13501 ggccgggact acctgatggc cttcctggcg cccgagctcc aggtggcgct cctcaccgtg
    13561 cacgtcccga tgcgcgaggc gctggacgcg gtcgagccgg tgacggtcac cgaggccctg
    13621 gagtgcctgc accgccacgc cggaggcaag atcgccctgg cggggctgaa cccgcacgcc
    13681 ggcgaggggg ggctcctcgg cgaggaggag gggcgtctcc tggtgccgac cgtggcggcg
    13741 gcgcgggagc gggggatcga cgtccacggc cccgagagcg ccgattcgct cttcgcccgc
    13801 gcccgccgcg gcgagttcga ctgggtcctg gcgctctacc acgatcaggg cctgatcgcc
    13861 gtgaagaccg ccgccttcgg cctcgccacc aactggaccc tcggcctccc cttcctccgc
    13921 acctccgtcg accacggcac cgccttcccc ctcgccggca aaggcatcgc cgacgccggc
    13981 cccatggaag ccgtcatcga gacgacgctg gagctgatct cggggacgct gccgcggaag
    14041 cgaacgaccg agtagcctta ccttttcaga gggtggcgat tggaaagccg agttgccggg
    14101 ctgtctcggt cagccctgag tcatagctcg caatgagaat cgatgcgccc tgagcccgca
    14161 ggtagacagc cgtggccaga tgcagcgcgt caagagtcct gacggagtgg gggaagggtt
    14221 cgagagcccg cgcgaggacc ggcggcacca tggcgacgag agcggtccgt ccgagaatcc
    14281 actgggcagt cgcggaatgc gattgcgtga cgccccgggc gtgaatccgt gtccagagct
    14341 catactcgag taaccggctg gacgtaagct cctcgtccca gagcgaagct ggcggacgcc
    14401 gattctccgc gaaaaggtga gcgagagcca cagaggtgtc gagatagatc aacgatcggc
    14461 ccggtcttcg tccaactcgc gcaggatgtc ttccagaggc gccactggcg gaggaagcgg
    14521 gggcgcaggc tccttggcca gcgctggagg cgtgatccat cccttgcgaa cgccatcgag
    14581 cagtgccggg ttcatcgccg ggaggtcacc gctttcccgg ggaggcacca actcggcgat
    14641 cacgcggtca tgctcggtga cgaggatggt ctctccctcc tgtacaaggt gcagaaactc
    14701 gtctaactgg tcttcgaggg cccttcgcgc caccgctcgc atgtctcgac cgtagccttc
    14761 cggaagaacg gtgtcaagcg ggaaccgcca ccagcgggtt gcacccgccg gtggctcatc
    14821 ccatctaaga ggtaggccct cctacccctc cccctccaca cctgcggcca cccgctcgtc
    14881 gaggatcgcc ccgcaccgtt ccagcgtgac cgaccctgcc gcctccagca gccaggcgag
    14941 cgactccggc gcctggagca ggtggatcgc cacgttcatg gcgtcgatga gagcctcgtc
    15001 gaagagctgg agatggccca cgacctctcc gccgtccgca ccgggcagga ggaccagcgg
    15061 atagacaccg tccgcgtccg gctgcttttc gaggcggatc agcggatcgc gccccgtgcc
    15121 ggggagcacg gcggcggcga cgagggcgag ccagcgggac gggtacaggc ccacgggcgc
    15181 gaagccccgg gccaggctct ccccgatccg gaacaggccg taacccgagc ccccgttcca
    15241 ggccaccggc tcgacccgcc acggcccggc gacatcggcc tcgcccgccg tgggaggctc
    15301 gtcccgctcg gccatgcggc cgaggaactg ggtggtgaag gaattcgggt ggatagacga
    15361 cgtgctacgc tcgtcaaagc tacgcataag tcctcccttg gactgtgtgt ggtcaggtcc
    15421 ggaagggagg tggtgctcca cttccgggcc gtcagccatc attggccgac caccctgcgt
    15481 actgcctatg cccgaatcct agaacgggga agcggcagaa atcaaccccc tttttgcggt
    15541 cttcttccgg taccctggac tccagggcac gtccaggcac ggcggccccc gctcaggcgc
    15601 cgcgcccgaa tcgcgtacat cagttgtcaa agagcctgag gagcctggcg tcaaactacc
    15661 ccgctccctc gaaccagaag cataccacag gagcccgcat gatgattttg ttaacaagtt
    15721 aacggcctcc tttgtagcat ctcctccgcg ccgctgccat ggaggcagcg gcagtgggcc
    15781 tgcggaaggg ctcgatacca gaaaaggaga aagaccatgg cgacgagaag ggaaacgacg
    15841 aagaccggaa tcctggggac ctggcgggag ctgctcccgc cgctgatggc caacgcggcc
    15901 gagctcccgc aactggagat cccccgcggc aagctggaga ggatcctggg acgggcgcag
    15961 gaagtcgtcc atctgcaggg ccttctggcc gcgaaccggc aggagctgac gagggagtgg
    16021 gacctcctgc aggcggaggg cgagcgagtg gcggcgctac tgcggcgagg gatcaaggag
    16081 cattacggcc cgcgcgccga gaagctggcg gagttcggcc tgcagccctt ccgcggccgg
    16141 aaggccaaga aggtcaccga ggccgaagag cccgacaccc ccaccctttc ctgatccctc
    16201 ccgtcatgct cgattcggat tccattcttt cgggggcgtg caccgcacgc cccctttttt
    16261 tgggctcaac cgcccggaac cctccgttcc ggagccgcgg cgtagcactt cgccgcggtc
    16321 tcggaacact ccggaagggt tccgtagtac ttccccaccc cgacgaagta gtacgacgcg
    16381 acgccgaacc actacggcgc caatcccgaa cacttcaaag cggctccgta ctactacgct
    16441 gccgtggcga actacttcgg agccgccctc gaacgcttca agatgacttc gtagtgcttc
    16501 ggcgccgcag tgaactgcta cgccgcagca gccgactact tcaaagtgat tccgtagtgc
    16561 ttcggagccc cagcgagcca ctacggcgcg gcagccaacg acttcggagc cacgccggaa
    16621 cactgcaaat tgatttcgta ctacttcggg acggcggcga agcagttcgc cgccgcgccg
    16681 aagtactacg gcgccgctcc tgaacgccgt aggaagagcc ggcgccccag gacctcccgg
    16741 tcccggagca gcggctctcg ctcattgaca actgaatcgt gacggggcgc cgccgtgtcc
    16801 aaggccgcgc ccctgaggat tcctccgtcc gacaggagga tggccggtag gcggaggtcc
    16861 gatccctccg cctcggggct accgctggga ggagagtaac cgctcggcgc cgctccggaa
    16921 cgcttcccgg cgcaccgaac gcaccgcccc cgtgcttcgg ctcgggctgc aatccaggct
    16981 agttccagcc ctcattgcag tacatgtcgc cagggccgat aaggacgaag agcaggtatc
    17041 taaccttttc gtcccaggtg ttatccgagc agaacaaggt catggtgata atggcgtcgt
    17101 tgtcgatcgc cgcggggtcc gagtaggtat ggcctacgta caggtcatcc caggttccag
    17161 tcccgtcccc gaagtcccag tagaccgcac tgactgaggc gttaccacag gtagtcgcag
    17221 tggtgtcgct ggtgtggccg ttgtcgtagg tccgattccc gtcgaagaca cagtaggctg
    17281 ggctggagct gtcgaaccgg atgcagttac cggagaagtt ggcgtacgtg gaggcactgg
    17341 ccgaggctgc ccaagcagca aaggtgaaga gggccgcgaa cacgactctt gaaggtctca
    17401 tctcttttct cctggagatt ggacgttgac ctcgaccccg tcaactgcaa caaggaacgg
    17461 ggactgcata ggtaccagac cgttagacct gggctgagtg caggtcgcca gccggcgact
    17521 tccgtcaagc tggccctctt catcggctgc cagttgctcg gcgtcgccct gatcctcgcg
    17581 tcgttcctga ggaagtgact ccctagcata ttgcgtaatg aagaattgga tgtaataacg
    17641 cggaacggca ggcccgaaga accggtctgt cagtggaccc ggatcggttc cctcggcgtc
    17701 gccgcgagcc caggggatgg aaccggaggc atcgtccaaa gcccgaggaa tcgttgccgg
    17761 ccgaggagcc gggcgcgctc aacatcgagg ggctggcggc gaaggacggc aagctcctga
    17821 tcggcttccg cggtccagtc cgcggcggga aggccctggt cgtgacgctc gagaacccgc
    17881 aggaggtcct caaccagggg gcgccagcct ccttcgccac gcccatcgag ctcgacctgg
    17941 acggcctcgg catccgcagc ctcgagcact ggcctgctga agcgagaagg tcctgcaccg
    18001 cgacatccct ctcgccggtg cagggcctca gggttgaacc gaaagcggcc tacgaggcca
    18061 tgtcgacgcc gcggacggat ggggctgcgg gagggcggag gctcgcgtcc tccatcctcc
    18121 ccgccagcca ggaggtggcg acggcctacc tgttgatgtg gacgtgctgg tgctcgttcg
    18181 ggttgctcgg cggatagctc cacttgaccg cgcctgggtt cgatccgtag acgccggtat
    18241 tgcggaggta ttcgccattc aagcccacgt gcagattgct ccccttgatg ctgaacagga
    18301 ggaacgcatt gtcggaggcg aggccggcct tgtagaggag ccaggtgttg aagatggtgt
    18361 ccaccgagat cgagcccccc ttggggatgg tgaacgacga gagactgatc aggtaaaagc
    18421 cgggattgat gtcgttgttg atccggctcg cgtccggctt ctggacgacg aactgccagc
    18481 tcttgccgtc gtaagccatg acatagtcga gcgagagatc ctcctgcgtc aggttttcga
    18541 gcgcgaaaac agtacccatg atcgccagtc ctttcaactc gcatcccgtc atcatgacga
    18601 gggcgttgtg actgggtact gtcgcgagac cgccgggtat ctcacgccct tttcacgggc
    18661 cgcgccccgg gacgggctga gcgcaagcga aaaggccccg cgccgcgaga tctcctctcg
    18721 cggggcgggg cctccgggtt gattcaaaag cggcttaccg ggccacggcc ttgcgcgggc
    18781 cggcagcctt gatctcttcg ctcacacccg cctcgtactt ctcgaagttg tcggcgaaca
    18841 tgtcggcgag ctggctggcc ttggcgtcgt agtccgccgg gttctgccag gtctcgcgcg
    18901 gacgcaggac gttgtccggc accccctcga cgtgcaccgg gacgttcagg ccgaagatcg
    18961 ggtcggtcca ggtctcga
//
LOCUS       DASPJI010000043         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_174353, whole genome shotgun sequence.
ACCESSION   DASPJI010000043 DASPJI010000000
VERSION     DASPJI010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174353"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VEL74_01220"
     CDS             <1..1176
                     /locus_tag="VEL74_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HYG61174.1"
                     /translation="LRERLRRSLPAYMVPAAWVVLPELPLNPNGKIDRLALPAPEAAA
                     SPFAVPPRDDLERRLAAIWEEVLGTGPVGVTDDFFDLGGHSLLAVRLMARVEAELGRA
                     LPLSALFQSGTVAGLAAGLREEEPAGSPLVLLQPRGSRPPLFLVHPAGGEALCYSGLV
                     RRLGTDQPVYGFEDTAEAARSVSALAAAYVAEVLAVQPEGPYRLAGWSFGGRVAFEMA
                     RQLTSHGRAVAFLGMIDTGQAVPDEEVDLSETGLLREILRGASREILDGLPPGLDDPL
                     GEVLERLRAAGQIPEGFDPATTRRLWQVFRRHVEMARRERPLPWTGRLVYYAAEEGLA
                     ADPTQGWAALAASVEVRRVPGDHVSMIYDEANLEVLARHLRAGLDASGSGLANPATR"
     gene            1226..2311
                     /locus_tag="VEL74_01225"
     CDS             1226..2311
                     /locus_tag="VEL74_01225"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HYG61175.1"
                     /translation="MAHVGVIFGGRSVEHQVSIVSARTVVRGLREAGHLVTPLGIAQD
                     GCWIDPAAAEAVLASGRRTTIEPLGQPVAPTLTHLISSGVEVLFPIVHGTWGEDGTLQ
                     GLCEMLDLPYVGPGVTASALAMDKLLFKRQMEAAGVPVVEYEAVTRRELEADPEAVLK
                     RLDRLPLPLFAKPSVGGSSVGVKKVTSRECQETSVRFALQFDDTVLVERGVAGRELEC
                     AVLGYGRLEASCIGEIVPGNDFYDYADKYLQDTAQLIAPADVPEAVAERLRELAVRAF
                     AAIGGAGLARVDFLVEGEDGIYVNEVNTLPGFTSISMYPRLWELSGVPLPRLVDRLVE
                     IARERHRDRHRLDAGIKQFLAQLERGE"
     gene            complement(2318..>2539)
                     /locus_tag="VEL74_01230"
     CDS             complement(2318..>2539)
                     /locus_tag="VEL74_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HYG61176.1"
                     /translation="FDMHALTAAHRTLPLGSRARVTNLENGETVEVQINDRGPHAQRR
                     LIDLSYGAAQELAMVNDGSALVEVVPVAP"
BASE COUNT          327 a          910 c          932 g          370 t
ORIGIN      
        1 ctgcgggagc ggctgcgccg gagcctcccc gcctacatgg tgccggccgc gtgggtcgtc
       61 ctgccggagc tgccgctcaa ccccaacggc aagatcgacc gcctcgccct ccccgcaccg
      121 gaagcggcgg cgtctccctt cgccgtgccg ccgcgcgacg acctggagcg gcggctggcg
      181 gcgatatggg aagaggtgct ggggaccggg ccggtcggcg tcaccgacga cttcttcgac
      241 ctcggcggcc actccctgct cgccgtgcgg ctgatggccc gcgtcgaggc cgagctgggg
      301 cgggcgctgc cgctctcggc gctgttccag agcggcaccg tggcgggcct cgccgccggg
      361 ctgcgcgagg aagagcccgc cggctcgccg ctggtcctgc tgcagccgcg gggctcgcgg
      421 ccgcccctct tcctggtcca cccggccggc ggcgaggcgc tctgttactc tgggctggtc
      481 cggcgcctcg ggacggacca gccggtctac ggcttcgagg acaccgccga ggcagcgcgg
      541 agcgtgtccg ctctggccgc agcctacgtc gctgaggtac tggcagtgca gcccgaaggg
      601 ccgtaccggc tggctgggtg gtccttcggc ggccgggtcg ccttcgagat ggcccgccag
      661 ctcacctccc acgggcgggc ggtcgccttc ctcggcatga tcgacaccgg acaggcggtg
      721 ccggacgagg aggtggacct cagcgagaca gggctgctgc gggagatcct gcggggcgcc
      781 tcgcgggaga tcctggacgg cctgccgccg ggcctcgacg atccgctggg agaggtgctc
      841 gaacggctgc gcgccgccgg ccagatcccg gagggcttcg atcccgcgac cacacggcgg
      901 ctctggcagg tcttcaggag gcacgtggag atggcgcggc gggagcgtcc gctgccctgg
      961 acgggccggc tggtgtacta cgccgccgag gaggggctcg ccgcggaccc gacccagggc
     1021 tgggcggccc tggccgcctc cgtcgaggtc cgccgcgtgc cgggggacca cgtctcgatg
     1081 atctacgacg aggcgaacct cgaagtcctg gcccggcatc tgcgggccgg gctcgacgcg
     1141 agtggatcgg gacttgcaaa tccggccacc cggtgatttg ctccctcatg gggagggaat
     1201 ggtcctgatc caataggaga tccttatggc gcacgtcggc gtcatcttcg gtgggcggag
     1261 cgtggagcat caggtctcga tcgtgtcggc gcgcacggtg gtgcgtgggc tgcgcgaggc
     1321 ggggcacctg gtcacgccgc tgggcatcgc gcaggacggc tgctggatcg accccgcggc
     1381 ggcggaggcg gtcctcgcga gcggccgccg gacgaccatc gagcccttgg gccagccggt
     1441 cgccccgacc ctcacccacc tgatctcctc gggcgtcgag gtgctgttcc ccatcgtcca
     1501 cggcacctgg ggcgaggacg gcaccctcca gggcctctgc gagatgctgg acctccccta
     1561 cgtcggcccc ggcgtgaccg ccagcgccct ggccatggac aagctgctct tcaagcgcca
     1621 gatggaagcc gccggcgtgc cggtggtgga gtacgaggcc gtcacccgcc gggagctcga
     1681 ggccgacccc gaggccgtcc tgaagcgcct cgaccgcctg ccgctccccc tgttcgccaa
     1741 gccgagcgtc ggcggctcca gcgtcggcgt caagaaggtg accagccgcg agtgccagga
     1801 gacttcggtg cgcttcgccc tgcagttcga cgacacggtg ctcgtcgagc gcggggtcgc
     1861 cgggcgcgag ctggagtgcg ccgtcctcgg ctacgggcgg ctcgaggcct cctgcatcgg
     1921 cgagatcgtc cccggcaacg acttctacga ctacgccgac aagtacctgc aggacaccgc
     1981 ccaactgatc gccccggccg acgtccccga ggccgtggcc gagcggctgc gcgagctggc
     2041 ggtgcgtgcc ttcgccgcca tcggcggcgc cggcctcgcc cgcgtagact tcctggtgga
     2101 gggggaggac gggatctacg tcaacgaagt caacaccctg ccgggcttca ccagcatctc
     2161 catgtacccc cgcctctggg agctctcggg cgtccccctg ccgcgcctcg ttgaccggct
     2221 ggtcgagatc gcccgcgagc gccaccgcga ccggcaccgc ctggacgccg gcatcaagca
     2281 gttcctcgcc cagctggagc gcggcgagta gaggtcttca aggggcgacg ggcactacct
     2341 cgaccaaggc cgagccgtcg ttgaccatgg ccagctcctg ggcggcgccg taggagagat
     2401 cgatgagccg gcgctgggcg tgcggcccgc ggtcgttgat ctgcacctcc actgtctcgc
     2461 cgttctcgag gttggtaacg cgcgcgcggc tgccgagcgg caaagtgcgg tgggcggcgg
     2521 tcagggcatg catgtcgaa
//
LOCUS       DASPJI010000044         5465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_803940, whole genome shotgun sequence.
ACCESSION   DASPJI010000044 DASPJI010000000
VERSION     DASPJI010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803940"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VEL74_01235"
     CDS             complement(<1..842)
                     /locus_tag="VEL74_01235"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HYG61177.1"
                     /translation="MDQSFSRDSSSPFADRSSDQREDYWRELYEARTYGYESSYEQLR
                     PAYLLGWRSRDSYGGAGWEDIERELAREWEIAWLSGGLPWEEVRWAVRDAWARAGAVS
                     GRWGGYYQPAYSYGRHDRGWNRSYERGSGDSQESYGGYGQGGDYGQQGYGSQQGYGQQ
                     GGASVGGAGEGYGGYRRSSRSLREELGERLTRHGQVDASRIEVKVDNGEVTLEGSVLS
                     RRQKRLAEEVAESVSGVRDIHNRLRIQAVSEEREKSAPPARSAPLAEESFSVGAPPPD
                     PPSKP"
     gene            complement(924..1883)
                     /locus_tag="VEL74_01240"
     CDS             complement(924..1883)
                     /locus_tag="VEL74_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006194567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HYG61178.1"
                     /translation="MRAAVIDGYGGVEKLEVREVPDPGAPGPGQVRVRVRASSLNPID
                     WKLRRGSYRLVLPAKFPKILGSDAAGEVEAVGPEVTRFEPGDAVFGQTAIRQAGAHAE
                     AVLMQESALAHKPEALTFEEAAAVPLAALTALQALRDKGELDSGERVLINGAAGGVGH
                     FAVQIARAIGARVTAVASGRNRDFLRQLGAERTIDYQTEDFAADEETYEVVFDAVGTS
                     SWQECELLLAEKGVYVSTQAGARQILDVAGTTVRGLFGEGRRAASVSVRPDAADLEVL
                     ARWIDQGRLRPHVERVFSLDEIRGAHEASESGHVRGKIVVRVG"
     gene            complement(1924..2397)
                     /locus_tag="VEL74_01245"
     CDS             complement(1924..2397)
                     /locus_tag="VEL74_01245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG61179.1"
                     /translation="MKIRRASIADAEELTQVAHESKSHWGYPPRWIELWRDDLTITPE
                     VIAGNELYLAEEDGTVLGFFGLIAGEGGGPVWTLEHFWVLPAAMGKGVGRTLFERARE
                     LAAGAGAAVLEIDADPNAAPFYERMGARRVGEVRSEIEGQPRVRPLLHLDLHPAA"
     gene            2505..3284
                     /locus_tag="VEL74_01250"
     CDS             2505..3284
                     /locus_tag="VEL74_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61180.1"
                     /translation="MMSKRVCLALALALICTVPGWAAREAFRAAPEIPYIDPAEVGLR
                     VERLGARVEGDLESPLGRLSFSATAQMDGRVTLEYELPEGRGTLVLFVDREAQTAEVF
                     YPEGVEIGPADRAAFRTLALALGSSLRDDSPEADLLLRAARLWGDHPLQPVRLTRIAA
                     PFAKSWTNLCGITSRSLAHDGSGHGLISETLAVGPRASNCRARCGAGCDALVGTSAWT
                     VDCGEHDRCEQHHSCCASCQDEFNSASDDYLFAPNCRLSGW"
     gene            complement(3403..3804)
                     /locus_tag="VEL74_01255"
     CDS             complement(3403..3804)
                     /locus_tag="VEL74_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61181.1"
                     /translation="MGLEQARQERPWPFRVILGVAHPKREAWVIAGFEPRNTREEARL
                     EEVRRELAFDPRVQSETLSAKAPGVPKNAKRVLDALVGGDSDREAACWTECGLDVLAE
                     RGRLSGLAGYLDEIRRELVPLFLGRGRKPPS"
     gene            complement(4017..4214)
                     /locus_tag="VEL74_01260"
                     /pseudo
     CDS             complement(4017..4214)
                     /locus_tag="VEL74_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611749.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            4562..5110
                     /locus_tag="VEL74_01265"
     CDS             4562..5110
                     /locus_tag="VEL74_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61182.1"
                     /translation="MRSSFEPNTPSVRPLPPNAFTADFLSRLAERDEPPTAGDADVAG
                     PWRIEAIPGYGFGLFRMGESLARGFTPTAVFPTRWLALLAAAILPGTGKDPLLLLSKQ
                     PDADGAYPLALAGGSGGDGGDGDGPAGWFRLFDEKAVDAINILIHLLQSPEALSYVLE
                     AAGSVTLERCGAILDERAASPE"
BASE COUNT          815 a         2019 c         1793 g          838 t
ORIGIN      
        1 ggcttggaag ggggatcggg cggcggggcg ccgacgctga aggactcctc ggccagcggg
       61 gcggaccgcg caggcggagc ggacttctcc cgctcctcag acacggcctg gatccgcaga
      121 cggttgtgga tgtcccgcac gccggagacc gattcggcga cctcctccgc cagccgtttc
      181 tgccgacggc tgaggacgct cccctcgagg gtgacctcgc cgttgtcgac cttgacctcg
      241 atccgcgagg cgtcgacttg tccgtggcgg gtcagccgct cgccgagctc ttcccggagg
      301 ctcctgctgg aacgtcgata gccaccatag ccttcaccgg caccaccgac gctggcgccg
      361 ccctgctgac cgtagccttg ttggctgccg tagccctgct ggccgtagtc gcccccctgg
      421 ccatagccgc catagctctc ctgggaatcg cccgagccgc gctcatagct tcgattccag
      481 ccacggtcgt ggcggccata gctatacgcg ggctggtagt agccgcccca gcggccggag
      541 acagcgccgg cgcgggccca ggcgtcgcgg acagcccacc ggacctcctc ccagggcaag
      601 ccgccgctga gccaggcgat ctcccactcg cgcgccagct ctctctcgat gtcttcccag
      661 cctgccccgc cgtaggagtc ccggctcctc cagccgagga gatacgcggg ccggagctgc
      721 tcgtagctgc tctcgtaccc gtaggtgcgg gcctcgtaca gctcgcgcca gtagtcctcg
      781 cgttgatcag agctccggtc ggcaaaaggc gacgaggaat cgcggctgaa gctttggtcc
      841 atggtccggt cctcctccag ttccaggcta tgcccgaccg gcggaggccg tcaatgaaat
      901 cgcggaatgc gtatgaacgg cctctagccg acgcggacca cgatcttgcc gcgcacgtgc
      961 ccggactcgc tcgcctcgtg cgcgccgcgg atctcgtcca gggagaagac ccgctcgacg
     1021 tgggggcgga ggcgcccctg gtcgatccag cgggcgagga cctcgaggtc ggcggcgtcc
     1081 gggcgcaccg agacggaggc cgcccggcgc ccctccccga acaggccgcg caccgtggtg
     1141 ccggcgacgt cgaggatctg gcgcgccccc gcctgggtgg agacgtagac ccccttctcg
     1201 gcgaggagga gctcgcactc ctgccaggag ctggtgccca cggcgtcgaa gaccacctcg
     1261 taggtctcct cgtccgccgc gaagtcctcc gtctggtagt cgatcgtccg ctcggctccg
     1321 agctgccgca ggaagtcgcg gttccggccg ctggccaccg ccgtcacccg ggcgccgatg
     1381 gccctggcga tctgcaccgc gaagtgcccg acgccgccgg ccgcaccgtt gatcagcacg
     1441 cgctcgccgg aatccagctc ccccttgtcg cgcagggcct ggagggcggt gagggccgcc
     1501 agggggacgg ccgcggcctc ctcgaaggtg agcgcctcgg gcttgtgggc gagggccgac
     1561 tcctgcatca gcacggcctc ggcatgggct ccagcctggc ggatggcggt ttggccgaag
     1621 accgcgtcgc cgggctcgaa gcgggtgacc tcggggccga ccgcctccac ctctccggcg
     1681 gcatcggacc ccagaatctt ggggaacttc gccggcagga cgagccggta cgagccccgc
     1741 cggagcttcc agtcgatcgg gttcaggctg gaggcacgca cccggacccg cacctggccc
     1801 ggacccggag cgccggggtc cggaacctcc cgcacctcca gcttctccac cccgccatac
     1861 ccgtcgatca ccgccgctcg catggttccg gccctccgtc tgtcgagagc cgtccactct
     1921 atctcaggcg gccggatgga ggtccaggtg gagcaacggc cggacccgcg gctgaccctc
     1981 gatctccgag cggacctcgc ccacccggcg ggcgcccatc cgctcgtaga aaggcgcggc
     2041 gttgggatcg gcgtcgatct cgaggaccgc ggcgccggcc ccggccgcca gctcccgggc
     2101 gcgctcgaac agcgtccgcc ccactccctt gcccatcgcc gccggcagga cccagaaatg
     2161 ctccagcgtc cagaccggac cgcctccctc cccggcgatc aacccgaaga agcccaggac
     2221 cgtgccgtcc tcttcggcca ggtagagctc gttgccggcg atgacctccg gcgtgatcgt
     2281 caggtcgtcg cgccacagct cgatccagcg cggcgggtac ccccaatggc tcttcgactc
     2341 gtgggcgacc tgggtcagct cctcggcgtc ggcgatcgat gcgcggcgga tcttcatcgg
     2401 ccctcccgga tctcggaatc attatgacaa cgatccccgt ctccccatag gctcgcccgg
     2461 caggccggtc cgcacccctt cctgatagag aacaaggagg attgatgatg tcgaagcgtg
     2521 tgtgtctggc gttggccctg gctttgatct gcaccgtccc cggatgggcg gcgcgggaag
     2581 cgtttcgcgc ggctccggag attccctaca tcgatcccgc cgaagtgggg ctgcgggtgg
     2641 agcggctcgg cgcccgggtc gagggggacc tcgagtcacc cctggggcgt ctgtccttca
     2701 gcgccaccgc gcagatggac ggccgggtca ccctcgagta cgagctgccg gagggccgcg
     2761 gcactctcgt cctgttcgtg gaccgcgagg cgcagaccgc cgaggtgttc tatcccgaag
     2821 gcgtcgagat cggtcccgcg gaccgcgccg ccttccgtac cctcgccctc gccctgggca
     2881 gctcgctccg cgacgactcg ccggaagccg acctcctgct gcgggcggcg cggctctggg
     2941 gggaccaccc gctccagcct gtccgcctga cccgcatcgc ggcgcccttc gcgaagagct
     3001 ggacgaacct ctgcggcatc acctcgcgca gcctggccca cgacggcagc ggtcacggcc
     3061 tgatcagcga gaccctggcc gtcggccccc gggcctccaa ctgccgagcc cgctgcggcg
     3121 ccggctgcga cgccctggtc ggcacgagcg cctggaccgt cgactgcggc gagcacgacc
     3181 gctgcgagca gcaccatagc tgctgcgcct cctgccagga cgagttcaac tcggcgtccg
     3241 acgactacct cttcgctccg aactgccggc tttcgggctg gtaagggtcg atccggacgg
     3301 ctcggaagca ccccgggccg tccggacgcc actccgaatt tgagccgaag gggaggtggt
     3361 gcctcccccc ctgggcggtg gcgcgggagg gagcggggga ggtcaggagg ggggttttcg
     3421 acctcgcccc aggaacaacg gcaccaactc gcggcggatc tcgtcgagat aaccggccag
     3481 accgctgagc cgcccccgct ccgctaggac atcgagccca cactccgtcc agcacgccgc
     3541 ctcccggtcg gagtccccac caacaagagc gtcaagaacc cgcttcgcgt tcttcgggac
     3601 acctggagcc ttcgccgaaa gggtttcgga ttgcacgcgc gggtcgaagg caagctcccg
     3661 gcgcacctct tcgagacgtg cctcctcccg ggtgttccga ggctcgaagc cggcgatgac
     3721 ccaggcctcg cgcttggggt gggcgacgcc gaggatgacc cggaacggcc aggggcgttc
     3781 ctgccgggcc tgttccaatc ccacgcggcg ttcctcaaag ccatccgtat cccggatcag
     3841 gaggaccgcg tcgggtggat gggcctcggt cgccaggagg aggtggacct gccccggttc
     3901 ggtggacagt ttaggacttg ggttcttcgt tcttctcttc ggggggcctg gggggcagag
     3961 ccccccagca ccacagtccc acggaaacgc gtagcgcgcc gcgacttagc gttcatctac
     4021 gccgcctcct ggagcttttc gaactcggcg gggcttagat agaccagggc cgaatggcgc
     4081 cggtgaaggt tgtaccagcc ctcgaggaac tcgaagaccg ccatccgtgc ctcgaccgga
     4141 ttgcggaaac gacgacggtc gagcagctcg cactccaacg ttgcaaagaa gctctcgcac
     4201 atcgcgttgt caaagcagtg aaccccaggc tcctttatgc ctgcgccgct tcgcggctac
     4261 tgaggtggct cgcggatatc atacgacttc ccccggctcc cctccacgcg taaatccgcc
     4321 gatccgatag caggcgcaac gaagatgaaa gaaccgccct ctcccctatt gacttcttcc
     4381 ggagcccgga tctaggctct cgtcattcgg ccccaccctg agacgacccg cgagcccgcg
     4441 ggttttcgtc gaggggtgcc gttcgaacca gggtcggccg gcaatgatgc cggctagcgg
     4501 cctggagggg aggtggctct ccccgcccag gcctgaccac acacagtcca tcggaggact
     4561 tatgcgtagc tcattcgagc ctaacacgcc gtccgttcgc ccccttcccc ccaacgcctt
     4621 caccgccgac ttcctgagcc gtctggcgga acgggatgag ccgcccaccg ccggtgatgc
     4681 cgacgtcgcc gggccgtggc ggatcgaagc catcccggga tacggcttcg gcctgttccg
     4741 gatgggcgag agcctcgccc gcggcttcac ccccacggcc gtcttcccca cccgctggct
     4801 cgccctcctc gcggcggcca tcctcccggg cacgggcaag gacccgctcc tcctcctctc
     4861 caagcagccg gacgccgacg gcgcctatcc tctggctctc gccggcggca gcggcgggga
     4921 cggaggcgac ggcgacggac cggcaggatg gttccggctc ttcgacgaga aggcggtcga
     4981 cgccatcaac atcctcatcc acctgctgca gtctccggaa gcgctgtcgt acgtcctgga
     5041 ggcggccggc tccgtgacgt tggagcggtg tggcgcgatc ctcgacgagc gcgccgcttc
     5101 gccggagtag cccaccccta cggacaccgt ttgacccggg atcgacacgg tttgacccga
     5161 gatcgacacg gttcgcccct cggggcaagc cgctcgaccc acggggcaag ccgttcgacc
     5221 ctcggggcga accgctcgcc ccgagatcaa cacctctcgc cccaagggtc agaccgctcg
     5281 acccttgggg cgagacgctt gacccgaggg gcagacggct tgacccacgg ggcgagtcac
     5341 ttgacccaga gggcgagtca cttgacccag ggggcgagac gcttgacccg cggggcagac
     5401 ctctccggtc tcggggcgga tcgctcgccc cgcggggcaa accgcgccaa aaggaaacgc
     5461 caccg
//
LOCUS       DASPJI010000045         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_174355, whole genome shotgun sequence.
ACCESSION   DASPJI010000045 DASPJI010000000
VERSION     DASPJI010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174355"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VEL74_01270"
     CDS             <1..833
                     /locus_tag="VEL74_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61183.1"
                     /translation="GTRRANYTKSRGEGKGWNLKPAHEWVTSEVEAIVPEETWNAANA
                     LIAEKRQGKTRTARRPVHLFAGLTFCECGGKMYVPSNTPKYVCQSCRNKVPQVDLERV
                     FQEQLKQFSLSPEEVASALEEADEALAGKGELLELLRREAARLKEEADQVYRLYLAGE
                     LTPRGFGERNTPIEERRAQLEEEIPRLQGEIDFLKIQHLSQEEVASQAEDLHARWDDL
                     SPDDRRGIVQAIVERVTIGKDEIEISLAYLPRTPPPPSPPLLKMVATDARIPRGSSLP
                     "
     gene            complement(781..1857)
                     /locus_tag="VEL74_01275"
     CDS             complement(781..1857)
                     /locus_tag="VEL74_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HYG61184.1"
                     /translation="MLAQATAATPWGIEARPVQVEVDVNNGLPQVQIVGLADAAVRES
                     RERVRAAIKNCGFDLPPKSVIINLAPADLKKEGNHLDLAIALALLAAHEQVPEESLSG
                     RLLCGELGLDGTIRSVRGGLAIADLAHRLGTREVLLPAANAGEASALGSVPVVPIRSL
                     AEAIHHLLGVEPLPPVAPSALSAQAESAIPDLSEVRGQEMAKRALEVAAAGGHNLLFI
                     GPPGSGKTMLARRLPGLLPPLTLAESIAVTKIHSLVAEEPPAGLLWRRPFRSPHTGTS
                     SAGMVGGGSNPRPGEASLSHNGVLFLDELPEFRRDTLEALRQPLEEGLVSVVRARARL
                     QFPARFALMAAMNPWGFVHQLPPS"
     gene            complement(1985..2410)
                     /locus_tag="VEL74_01280"
     CDS             complement(1985..2410)
                     /locus_tag="VEL74_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYG61185.1"
                     /translation="MILSESKAGVAAPPRRSTLSIVLWVIQVLLALLFLWAGGMKLVL
                     PLEKLAGPVALPGLFVRFIGAVEVLGGLGLILPGLLRIRPILTAWAAAGLAIIMIGAV
                     GITLASGSVGMAAIPLVTGLLAAFVAYGRWRLAPLLSRS"
     gene            2581..>4027
                     /locus_tag="VEL74_01285"
     CDS             2581..>4027
                     /locus_tag="VEL74_01285"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HYG61186.1"
                     /translation="MRRPLAPDDLLYRDPSSQAIALPDGRLLVRHARGLSLLHGVDAG
                     DLRRALGGEGGLRVADVVADLEETYGKDGALRLLSSLIGELVHVAEPVKPAVDAAGSG
                     EVPPPLPWLAGGTVAVVGTGPAARRFAERLERAGSLSVPLLEPGAIRDGERLRGVALA
                     VCIPEEATYRDLFTLQRACLAAGVPSLFLTADPDGLRAGPLTVPGVSPCLACAQAAAF
                     RFLRLEPSTLLAALEQFRTGEAGSGFPRAEAALADETRRVLAGGEPSLLTEVLWLTPG
                     GEARRLPVERVPGCPVCGGEEAPSVEDPLAARASLVVVETQDRAVSRAFPADPEGLVT
                     SVGILGGGTAGYLAAMALRRKVPHVEVTLIESSDVPVIGVGEATTPLMPQFLHVDLGL
                     DVHQLFREVQPTFKLGIRFLWGEPGTGDFTYPFGPVHLLEPAVYGGDVRDCSLQSLLM
                     MAGAMPIYKEGAAWTSRMGTEVAYHLDNERFV"
BASE COUNT          623 a         1420 c         1400 g          584 t
ORIGIN      
        1 agggcacccg ccgcgcgaac tacacgaaga gccggggcga gggcaagggc tggaacttga
       61 agcccgccca tgagtgggtc acctctgagg tggaggcgat cgtccccgag gagacgtgga
      121 acgcggcgaa tgccctcatt gccgagaagc gccaagggaa gactcgtact gcccgccggc
      181 cggttcatct cttcgccggt ctcaccttct gtgagtgcgg cggcaagatg tacgtgccct
      241 cgaacacccc gaagtacgtc tgccagtcct gccgaaacaa ggtgccccag gtggacttgg
      301 agcgggtctt ccaggaacag ctcaagcaat tctccctctc gcctgaggag gtcgcctccg
      361 cgcttgaaga ggcggatgag gcgctggccg ggaagggcga actcctcgaa ctcttgcgcc
      421 gggaggcggc gcgtctcaag gaggaggccg atcaagtcta ccgcctctac ctcgccgggg
      481 agctcacccc ccgagggttc ggagagagga acacgccgat cgaggagcgg cgggctcagc
      541 tcgaagaaga gatcccccgc ctccaaggcg agatcgactt cctgaagatc cagcacctat
      601 cccaggagga ggtcgcctcc caagcggaag acctccatgc ccgctgggac gatctctcgc
      661 ctgatgaccg gagagggatc gtgcaagcca tcgtggagcg ggtgaccata ggcaaggacg
      721 aaatcgagat ctccctggcc tacctgccgc gcacaccccc gcccccatca ccccctcttc
      781 ttaagatggt ggcaactgat gcacgaatcc ccaggggttc atcgctgcca tgagggcgaa
      841 gcgggccggg aactgcagcc gggcgcgcgc ccgcacgacg ctcaccaacc cctcttccag
      901 cggctggcgc aacgcctcca gggtgtcgcg ccggaactcc ggcagctcgt ccaggaaaag
      961 cacgccgtta tgggagaggc tcgcctcacc gggccggggg ttgctgccgc ctcccaccat
     1021 gccggcggag ctggtgccgg tgtgcgggct gcggaacggg cggcgccaga gaaggccggc
     1081 cggcggctcc tccgccacca gggagtggat cttggtgacg gcgatcgact cggccagcgt
     1141 cagcggcggc agcaatcctg gcaggcgccg ggcgagcatc gtcttgcccg agcccggcgg
     1201 gccgatgaag aggagattgt ggccgcccgc cgccgccacc tccagcgccc gcttcgccat
     1261 ctcctggccg cggacctccg acagatccgg aatcgcgctc tcggcctggg cggagagcgc
     1321 ggacggcgcc accggcggca gcggctccac cccgagcagg tggtggatgg cctcggcgag
     1381 gctgcggatg gggaccaccg gcaccgaacc cagggcggac gcctcgccgg cattggccgc
     1441 cggcagcagg acctcccggg ttcccagacg gtgcgcgagg tcggcgatcg ccaggccgcc
     1501 gcgcaccgag cggatggtac cgtcgaggcc cagctcgccg cagaggagcc gtccggacag
     1561 ggactcctcc ggcacctgct catgggccgc cagcagagcc agggcgatgg cgaggtcgag
     1621 gtggttcccc tcctttttca ggtcggccgg ggccaggttg atgatgaccg acttgggcgg
     1681 caggtcgaaa ccgcagttct tgatcgccgc gcggaccctc tcgcggctct cgcggactgc
     1741 ggcatcggcc agaccgacga tctgcacctg cggcagaccg ttgttgacat cgacttcgac
     1801 ttggacaggg cgggcttcga ttccccaggg ggtagctgcg gtcgcttggg ctaacatgcc
     1861 cctaaaagac ggaaaacgcc ggaaaaatct tgcacttttt ttcggcggtc ctgccggaag
     1921 acggtaaacc tctgagtgac agaggtttac ctcttcaagc ggcaccagtc cgtgcccctc
     1981 ccggctacga gcggctgaga agcggcgcga gccgccagcg gccgtaggcg acgaacgcgg
     2041 ccaggagccc ggtaaccaga gggatcgcgg ccatcccgac gctgccgctg gcgagcgtga
     2101 tgcccaccgc gccgatcatg atgatggcga ggccggcggc ggcccaggcc gtcaggatcg
     2161 gccggatgcg caggagcccg gggaggatca ggccgaggcc gccgagcacc tcgacggcgc
     2221 cgatgaagcg cacgaacagg cccggcaggg ccaccggccc ggccagcttt tccaagggaa
     2281 ggaccagctt catccctccg gcccacagaa acagcagggc gagcagcact tgaatcaccc
     2341 acagcacgat gctcagggtc gatctcctcg ggggtgctgc gacgccggcc ttcgattcgg
     2401 acaagatcat caccgttcct cctgggttga gtgtttcggg gagccaaggt gtctcgggca
     2461 cccctgactc tacctcttgt cgaaccggga gcggcgaaat cgacagggtg gtcctcctgg
     2521 cttgcgctcg cccgcgattc gcggcactct tcccgaccgg aacgagaccg agagacgacc
     2581 atgcgccgcc cccttgctcc cgacgacctc ctctaccgcg acccatccag ccaggccatc
     2641 gccctgcccg acggccggct gctggtccgt catgcccgcg gcctgagcct gctgcacggc
     2701 gtcgacgcgg gagacctgcg gcgtgcgctc gggggggagg gggggctgcg ggtggcggac
     2761 gtggtcgccg atctggagga aacctatgga aaggacggtg ccctccgtct gctctccagc
     2821 ctgatcggcg agctggtgca cgtcgcggag ccggtgaagc cggcggtgga cgcggcgggc
     2881 tccggcgagg taccgccgcc gctgccctgg ctcgccggcg gaacggtggc cgtggtcggg
     2941 accggcccgg cggcccgccg cttcgccgag cggctcgagc gtgccggctc cctttcagtg
     3001 ccgctgctgg agcccggcgc tatccgcgac ggcgagcgcc tgcgcggggt ggcgctggcg
     3061 gtctgcatcc cggaagaggc gacgtaccgg gacctcttca ccctccagcg ggcgtgcctg
     3121 gccgcggggg tgccgagcct gttcctgacc gcggacccgg atggcctgcg cgccggcccc
     3181 ctcaccgtac cgggcgtcag cccctgcctc gcctgcgccc aggccgccgc cttccggttc
     3241 ctgcggctgg agccctccac cctgctcgcc gccctcgaac agttccggac gggagaagcc
     3301 ggctccgggt tcccccgagc tgaggcggcg ctggcggacg agacccgccg ggtgctggcc
     3361 ggcggcgagc cctccctcct gaccgaagtc ctctggctca ctccgggcgg cgaggcgcgg
     3421 cgcctgccgg tggagcgggt gcccggctgc ccggtctgcg gcggcgagga ggcgccgtcc
     3481 gtcgaagacc cgctggccgc gcgagcttcc ctggtggtcg tggagacgca ggaccgcgcc
     3541 gtctcccggg cgttccccgc ggaccccgaa ggcctcgtga cctccgtcgg catcctgggc
     3601 ggcggcaccg cgggatatct cgcggcgatg gccctccgcc gcaaggtgcc ccacgtcgag
     3661 gtgaccctca tcgaatcgag cgacgtgccg gtcatcggcg tcggggaggc gacgacgccg
     3721 ctcatgcccc agttcctcca cgtggacctc gggctcgacg tccaccagct cttccgcgag
     3781 gtgcagccca ccttcaagct gggcatccgc ttcctctggg gcgagcccgg gaccggcgac
     3841 ttcacctatc ccttcggccc ggtccatctg ctggagcccg cggtctacgg cggcgacgtc
     3901 cgcgactgct cgctccagtc gctgctgatg atggcgggcg ccatgcctat ctataaggaa
     3961 ggggcggctt ggacgtcgcg gatgggcacc gaggtcgcct accacctgga caacgagcgc
     4021 ttcgtcg
//
LOCUS       DASPJI010000046         4095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_60346, whole genome shotgun sequence.
ACCESSION   DASPJI010000046 DASPJI010000000
VERSION     DASPJI010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60346"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..2877)
                     /locus_tag="VEL74_01290"
     CDS             complement(106..2877)
                     /locus_tag="VEL74_01290"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYG61187.1"
                     /translation="MPLGSVPDGGAPPLPAPSPLGRGTPIGRYLILDVVGTGGMGVVH
                     AGYDPELDRKVALKLLRPDRDAAGRGEAHRLRLLREAQAIARLSHPNVIQVYDVGTFE
                     DRVFIAMELVQGRSLRQWLREAPRSWREVTGLFSLAGWGLAAAHEAGLVHRDFKPDNV
                     LLGDDGRVRVADFGLAREAREAPEVIGEAGGEEDRPPPDGEAVGKGSGNLALPLTEWG
                     VVVGTPAYMAPELFRGERADARSDQFSFCTALWEALYSERPFDGGDARELAEAYARGA
                     APEPPARTEVPAWLRQVLQRGLSLDPAARYPSMDELLRELDRDPVAARRRWSRAAAVA
                     AVSCLAVAGVAQWGWSRAQLCRGGEDSLARVWNDEARGAGRAAFRATGLPFAEGAWTG
                     AERLLDRYARDWTRLHRESCEATRRRGEQSEELLDRRMACLDQRLGELRALSALFARA
                     DAGVVEKAERATNRLVDPAVCSDVEALTARVPLPREPERRARVEELQARLAEAKVLSD
                     AGKEREALARLRPLVAAARSTRHRPLEAEVLYLAGYVEEKLGESRAAEATVFEALLAA
                     HAGGHREAMADAAAQLSWVIGYGQARAVEGQRWARLAQAMLEGLEGSEALRARVANRH
                     GGWLLSAGRYEESAALFRQADRWAGQGLGPDNLVSAAALGNLAIALEQQGRWQEALEV
                     NLRALAMRRRLLEPDHPDLARSYNGLGNVHSKLGQKEKALEMYRAALRIFEASYGPGH
                     LQVAGLHGNIGSTLRESGRFEEAMPHYRRCLASLEKIEPEGPNVSWALSNLGMLLFDL
                     ERYEEALPVFQRSVALDEKLLGPSHPDLAYDLDGLGHVLHELGRPAEAVPLQERALAL
                     RVAANVSPILVADSRFGLARALWDVGSRDRALRLARQARATYAAQGEPWQQSLAAADA
                     WLRERERGR"
     gene            complement(3024..3971)
                     /gene="xerD"
                     /locus_tag="VEL74_01295"
     CDS             complement(3024..3971)
                     /gene="xerD"
                     /locus_tag="VEL74_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HYG61188.1"
                     /translation="MCRVAGPVLTTAADPPRRAPVPAWKRVLQRYLESLAVERGLSQN
                     SIDAYRRDLTRLGEALARKAGDLLTAGPQDLSAHLRDLTRQGLSPRSNARALSAIRGF
                     YEHLVEIKERADDPAVNLLPPKLWRSLPKVLSEAEVESLLAAPDVTTPLGLRDRAMLE
                     LLYATGLRVSELVGLQIPQLRLDVGFLVAFGKGSKERIVPVGEQAEQWVLRYLREVRP
                     ELAAGRHQTVFVNYVGDAMSRVGFWKILRGYAVELGIRELSPHVLRHSFATHLLEHGA
                     DLRAVQTMLGHADISTTQIYTHIHQQRLKSLYDRFHPRS"
BASE COUNT          557 a         1546 c         1463 g          529 t
ORIGIN      
        1 agccgaccac gtgccggccc gggccgagcg ggatacgccg cacggcggtc ccctcgcggg
       61 agaggacctt gaggatccag gcgccggcgg tgccgggcgc cccgctcacc ggcccctctc
      121 ccgctcgcgc agccaggcgt cggccgcggc cagggactgt tgccacggct ccccctgcgc
      181 cgcgtaggtg gcacgcgcct gacgggccag gcgcagggct ctatcccggc tgcccacatc
      241 ccacagggcg cgggcgaggc cgaaccggga atccgccacc aggatggggg agacgttggc
      301 cgccacgcgg agagccaggg cccgctcttg caggggcacg gcttcggccg ggcgtccgag
      361 ctcgtgcagc acatggccca gcccgtcgag gtcataggcg agatcgggat gcgacggccc
      421 taggagcttc tcgtccagag ccacggagcg ctggaagacg ggcaacgcct cctcatagcg
      481 ctccaggtcg aacaggagca taccgagatt ggagagagcc caggagacgt tggggccctc
      541 cggctcgatc ttctcgaggg aggccaggca tcgccggtag tggggcatgg cctcctcgaa
      601 gcgcccgctc tcgcggagcg tgctgccgat attgccgtgc agcccggcca cctgcaggtg
      661 gccggggccg tagctcgcct cgaagatccg cagggccgcg cgatacatct ccagcgcctt
      721 ctccttctgt cccagcttgg agtggacgtt gcccaacccg ttgtaggagc gggccaggtc
      781 gggatgatcc ggctccagga gccggcgccg catcgccagg gcgcgcaggt tgacctccag
      841 cgcctcctgc cagcggccct gctgttccag cgcgatcgcc aggttgccca gagccgcggc
      901 ggagaccagg ttgtcgggcc ccaggccctg gccggcccag cggtccgcct gccggaacag
      961 ggcggccgac tcttcgtacc tcccggcgct caggagccag cccccatggc ggttcgcgac
     1021 gcgggcccgc agcgcctcgc tcccctcgag cccttcgagc atcgcctgcg ccagccgtgc
     1081 ccagcgctgc ccttcgaccg cccgggcctg cccgtagccg atcacccagg agagctgcgc
     1141 cgcggcgtcc gccatcgcct cgcggtggcc gccggcgtgg gcggcgagca acgcctcgaa
     1201 gacggtcgct tcggccgcgc ggctctcccc cagcttctcc tccacatagc cggccaggta
     1261 gagcacctcg gcttcgaggg gacggtgtct cgtgctccgg gccgcggcga cgaggggacg
     1321 gaggcgcgcc agggcctccc tttccttgcc ggcgtccgaa agcaccttgg cctcggccag
     1381 ccgcgcctgc agctcttcga cccgggcgcg ccgctccggc tcccgcggca gggggacgcg
     1441 ggccgtgagc gcctcgacat ccgagcagac cgccggatcg acgagccggt tggtggccct
     1501 ctcggccttc tccactaccc cggcgtcggc gcgggcgaac agcgcggaga gggcccgcag
     1561 ctcccccagc cgctggtcca gacaggccat ccgccggtcc agcagctcct ccgactgctc
     1621 cccacgccgg cgggtggcct cgcaggactc gcggtggagg cgggtccagt cccgggcgta
     1681 gcggtccagc agccgctcgg cgccggtcca tgccccctcg gcgaagggga ggcccgtggc
     1741 ccggaacgcg gcgcgccccg ccccgcgggc ctcgtcattc cagacccggg cgaggctgtc
     1801 ctcgcctcca cggcagagct gggcccggct ccacccccac tgtgccacgc cggccaccgc
     1861 cagacaggag acggcggcga ccgcggcggc cctcgaccag cggcggcgcg ccgccaccgg
     1921 atcccggtcc agctcccgca gcagctcgtc catggagggg taccgggccg ccggatcgag
     1981 gctcagcccg cgctgcagca cctgccgcag ccaggcgggc acctccgtcc tcgccggcgg
     2041 ctcgggcgcc gccccgcggg cgtacgcctc cgccagctcg cgggcgtccc caccgtcgaa
     2101 ggggcgctcg ctgtaaaggg cctcccagag cgccgtacag aagctgaact ggtcgctgcg
     2161 ggcgtcggcc cgctcgccgc ggaacagctc cggcgccatg taggcggggg tgccgacgac
     2221 gactccccac tcggtgagcg gcagcgccag gttccccgag cccttgccca ccgcctctcc
     2281 atccggcggc ggccggtcct cctctccccc tgcctcgccg atgacctccg gcgcctcccg
     2341 cgcctcccgc gccagcccga agtcggccac ccgcacccgg ccgtcatccc ccagcaggac
     2401 gttgtcgggc ttgaagtccc ggtgcaccag gccggcctcg tgcgccgcgg ccagccccca
     2461 gccggccagg gagaagagcc cggtcacctc gcgccaggac cgcggcgcct cccgcagcca
     2521 ctggcggagc gaccgcccct ggacgagctc catggcgatg aacacccggt cttcgaaggt
     2581 ccccacgtcg tagacctgga tgacgttggg gtgggagagc cgggcgatgg cctgcgcctc
     2641 gcgcagcagc cggaggcggt gggcctcccc gcggccggcg gcgtcccggt ccggccgcag
     2701 cagcttcaac gccaccttgc ggtcgagctc gggatcgtag ccggcatgga cgacccccat
     2761 gccgccggtg cccacgacgt cgaggatgag ataacggccg atcggcgtgc cgcgccccag
     2821 aggggacggc gccgggagag gcggcgcgcc gccgtccggc acactaccca gaggcacggt
     2881 ctctgtttcc ggcgtcgtcg ctcccgaaag cgctcccgaa ccgggctccc ggccctcggt
     2941 cccctggccc atggcgacct ccgactcgtc aggtttcaag cgagaaacgc ttgtataccc
     3001 cggatctcct cagaggggaa gtgctacgag cgggggtgaa agcggtcgta cagactcttg
     3061 aggcgctgct ggtggatatg ggtgtagatc tgagtggtgg agatgtcggc gtggccgagc
     3121 atggtctgga ccgcccgcag gtccgccccg tgctccagaa gatgggtggc gaagctgtgc
     3181 cgcagcacat gcggcgagag ctcccggatc cccagctcta ccgcgtagcc acgcaggatc
     3241 ttccagaagc ccacccggct catggcgtcg ccgacgtagt tgacgaacac cgtctggtgc
     3301 cgccccgccg ccagctcggg gcggacctcc cggaggtagc ggagcaccca ctgctccgcc
     3361 tgctccccca ccggcacgat gcgctccttg ctccccttgc cgaaggcgac gaggaagccg
     3421 acatccaggc ggagctgcgg gatctgcaac cccacgagct cgctgacccg caggccggtg
     3481 gcatagagga gctccagcat ggcccggtcg cgcagcccca ggggggtggt cacgtccggc
     3541 gccgcgagga gcgactccac ctcggcctcg gagaggacct tgggcaggct gcgccagagc
     3601 ttgggaggga ggaggttgac ggccgggtcg tcggcccgct ccttgatctc caccaggtgc
     3661 tcgtagaagc cgcggatggc ggagagggcg cgggcgttgg agcggggcga gaggccctgg
     3721 cgcgtcaggt cgcggaggtg ggcggagagg tcctgggggc cggcggtgag gaggtccccc
     3781 gccttgcggg ccagggcctc gccgagccgc gtcaggtcgc gccggtaggc gtcgatgctg
     3841 ttctgcgaca gcccgcgctc gaccgccagg ctctccaaat aacgctggag cacccgcttc
     3901 cacgcgggca ccggcgcgcg gcggggggga tcggcggcgg tggtcaggac gggccccgcg
     3961 acacggcaca cggctcaggg cagggcgcgg ggccgcggct cctggggcgt cacgccgcgg
     4021 gaccggcaga tctccaggat ctcggtctct tccaggatgg ccagagcgga cttggcgcgg
     4081 ccgaagatct cctgg
//
LOCUS       DASPJI010000047         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_804045, whole genome shotgun sequence.
ACCESSION   DASPJI010000047 DASPJI010000000
VERSION     DASPJI010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804045"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VEL74_01300"
     CDS             <1..624
                     /locus_tag="VEL74_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61189.1"
                     /translation="EGKPLRLLHWSHMLAVKPEARDLHLGTRLKLYQRELLLPLRVEA
                     VQWTYDPLESRNAHLNLTRLGAEVEEYVEEMYADEMGSELARGIGTDRFVVEWRIAGE
                     RAGEALAGRPPAVPEHVAGAPVVNPAGAAVTDPGEELPATPGVRIEIPENIQALKTEN
                     PEWAWAWRISTRHAFEHYLDRGYRVVGFWREEGRCFYGLTAPGAPAA"
     gene            641..1765
                     /gene="menC"
                     /locus_tag="VEL74_01305"
     CDS             641..1765
                     /gene="menC"
                     /locus_tag="VEL74_01305"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HYG61190.1"
                     /translation="MLRIERITLLEVHLTLKEPFQISSGTQTHKRVLLAHLLSSDGAE
                     GWGECAAGSVPNYSYETVDTAWLALREWVAPRVLGRTFNGPEEIQPALDVGFHEHRMA
                     KAAVEMAGWDLAARAEGVSLAKKLGGSRDRIPVGISIGIQASPDVLVTKARAALERGY
                     RKVKIKIKPGSDFEFLSAARQALGPDAPLMADANNAYTLADVESLKRLDDLNLMMIEQ
                     PLAHDDLLRHVELQKQLRTPICLDESITSVDRAEDMIALGSGRIINIKPGRVGGFRQS
                     LAIHDLCARHGIPVWCGGMLEAGVGRAHNVALASLPNFTLPGDLSPSEHYWQKDIVIP
                     EWTMDREGWIQVPVDRPGIGVEIDRERIEAEAVRREELTA"
     gene            complement(2132..>2781)
                     /locus_tag="VEL74_01310"
     CDS             complement(2132..>2781)
                     /locus_tag="VEL74_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61191.1"
                     /translation="GWGAVGSGGRQKGGSILLAFAWLLTVLGFGLFWNNSDDQFYFQL
                     AVAFGALAARIPRGPILALSCVGLLWNLVDVGHRHVLYPRQERIALLEKEIRGACLVV
                     YPGFDEASMLLALSEAGRETARLPITNLATTLPPEEGTRALADRVRGCVETGGRVVLL
                     DLYDTPPDRNPWKFLRRLGYERSDLHAVLAGLPVDKASRRVGPFRVREAKSNTTS"
BASE COUNT          479 a          952 c          951 g          399 t
ORIGIN      
        1 gagggcaagc cgctgcgcct cctgcactgg tcgcatatgc tggcggtcaa gcccgaggcg
       61 cgggacctgc acctcggcac ccggctcaag ctctaccagc gcgagctgct cctccccctg
      121 agggtcgaag cggtgcagtg gacctacgac ccgctggaat cgcggaacgc gcacctcaac
      181 ctcacccggt tgggcgcgga ggtggaggag tacgtcgagg agatgtacgc cgacgagatg
      241 gggagcgagc tggcccgcgg catcggcacc gaccgcttcg tggtcgagtg gcggatcgcc
      301 ggcgagcggg cgggcgaggc gctggcgggc cggccgcccg ccgttcccga gcacgtcgcc
      361 ggcgcgccgg tggtcaatcc cgcgggagcc gcggtgactg atcccgggga ggagctcccc
      421 gccaccccgg gcgtgcggat cgagatcccg gagaacatcc aggcactcaa gacggagaac
      481 ccggagtggg cgtgggcctg gaggatctcc acgcgtcatg cattcgaaca ctacctggac
      541 cgcggctacc gcgtcgtggg cttctggagg gaggaggggc gctgcttcta cggcctcacc
      601 gctccgggag cgccggcggc ctgagccgga gggattgaac atgctgcgga tcgagcggat
      661 caccctcctg gaggtgcacc tcaccctcaa ggagcccttc cagatctctt ccggcacgca
      721 gacgcacaag cgcgtgctgc tcgcccacct gctctcctcg gacggcgccg aggggtgggg
      781 cgagtgcgcc gccgggagcg tccccaacta cagctacgag acggtggata ccgcctggct
      841 cgccctgcgc gagtgggtgg cgccgcgggt cctcggccgg acgttcaacg gtcccgagga
      901 gatccagccg gcgctcgacg tcggctttca cgagcaccgg atggccaagg cggcggtgga
      961 gatggccggc tgggacctcg cggcgcgggc cgagggggtg tcgctggcga agaagctcgg
     1021 cggctcgcgc gaccgcatcc ccgtcggcat ctccatcggc atccaggcga gccccgacgt
     1081 gctggtgacc aaggcccggg ccgccctgga gcgcggctac cgcaaggtga agatcaagat
     1141 caagccggga tccgacttcg agttcctgag cgcggctcgg caggcgctgg ggcccgacgc
     1201 gcccctgatg gcggacgcca acaacgccta cacgctggcc gacgtcgagt ccctcaagcg
     1261 gctcgacgac ctgaacctga tgatgatcga gcagccgctc gcccacgacg acctgctccg
     1321 tcacgtcgag ctgcagaagc agctccgtac ccccatctgc ctggacgagt cgatcaccag
     1381 cgtcgaccgg gccgaggaca tgatcgccct gggctccggc cgcatcatca acatcaagcc
     1441 gggccgcgtc ggcggcttcc ggcagtcgct ggcgatccac gacctctgcg cgaggcacgg
     1501 catcccggtc tggtgcggcg gcatgctgga ggccggggta gggcgggccc ataacgtcgc
     1561 cctggcgtcg ctgcccaact tcaccctgcc cggcgacctc tcgcccagcg agcactactg
     1621 gcagaaggac atcgtcatcc cggaatggac catggaccgc gagggctgga tccaggtgcc
     1681 ggtggaccgt cccggcatcg gggtggagat cgaccgcgag cgcatcgaag ccgaggccgt
     1741 gcgccgcgag gagctcacgg cttgaggtag gcaggggctg gggctcctcg ggagccccct
     1801 catggagtaa ccgtcagaag caccggcctg aacagtaatt gcaccttttt ccgacagggc
     1861 aatcctcgtc tgacgtgcag accggagcga tggtgcaggc ataagccggc ttgctcatga
     1921 gggctccgga cgcgagcagg acggccacgg cacaaagggc aagtctcttc atcgttctct
     1981 ccttactgga acagctcgcc aagccgcaag ctgtttcctt aatagcagaa gcataggtta
     2041 tttgcaatag aagaaatcga tcggaccgtt ccaacgccgc atcgtagcga tccaatgcgg
     2101 catcgaaccc ttccaacgcc gcgttgaacc cttacgatgt cgtattcgac ttggcctccc
     2161 ggaccctgaa cggccccacc cgccgcgagg ccttgtccac cggaagcccg gccaacaccg
     2221 cgtgcaggtc ggaccgctcg tagcccagcc ggcgcaggaa cttccagggg ttgcggtccg
     2281 gcggcgtgtc gtagaggtcc agcaggacga cccggccgcc ggtctcgacg cacccccgga
     2341 cccgatccgc cagggctctc gtcccttcct caggagggag cgtcgtcgcg aggttggtga
     2401 tgggaaggcg ggcagtctcg cggccggcct ccgagagggc cagcagcatc gacgcctcgt
     2461 cgaacccggg atagaccacc agacaggcgc cccggatctc cttctcgagt agggcgatcc
     2521 gctcctgccg gggatagagg acgtggcggt gtcccacgtc caccaggttc cagaggagcc
     2581 ccacgcagct cagtgccagg atcgggccgc gcggtatccg ggccgccagg gctccgaagg
     2641 ccacggcgag ttggaagtag aactggtcgt cggagttgtt ccagaagagc ccgaagccga
     2701 gcacggtgag gagccaggcg aaggcgagga ggatagaacc ccccttctga cgtcccccgc
     2761 tccctaccgc cccccaccca g
//
LOCUS       DASPJI010000048         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_804115, whole genome shotgun sequence.
ACCESSION   DASPJI010000048 DASPJI010000000
VERSION     DASPJI010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804115"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2150
                     /locus_tag="VEL74_01315"
     CDS             <1..>2150
                     /locus_tag="VEL74_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYG61192.1"
                     /translation="ALRFGIHTGPAVVWNRPGQADRLQLGSTLDLALGIQSAAPVNGI
                     AVSAMSRRLLARHFATRPLPPARLPNVDGPAEIYEAGEPLEGWQHEGAPVAPLVNREA
                     EIQILLDRFRLAGSGAGGQAVLIAGEPGIGKSRLIRALSEQLAAEAPIWLTAQGSTLT
                     QNTPLAPIAQLLTRALSGADDSTEGSDEERLRRLEEVLDEHDLPRPESTVLLGELLAL
                     PVADRYPPLALSPEARRKRTLAAILVFLGGLAERRPVVAVIEDLHWIDPSTLELLDLL
                     LGELPMLSLLLVATFRTEFSATWRPTAVTQLSLSGLNESQTKQLVAQVAEGKGLPAEV
                     HREIVVRTDGNPLFIEELTKAALEAEAPLHEPASVPLTLGASLLARLDRLGDAKEVAQ
                     LAAVLGRTFSLELLEALSWIKGASLSSALARLVQAEILYRQRRPRGVGYIFKHALIQD
                     VAYMSLLASDRQQLHRRLVGLFHEEFPEVAEAEPELMAHHCERGGLVVESVDYLLGAG
                     VRAAQRSAYLEAMSHLNRALDLLYSLPLAATPATAAELLERELSVRSMLIVVLEAVKG
                     WGAPEVAANAERCAVLCREVGDHASLIPALSSLWAHHLLRGDRQPTVDLADRIARLAE
                     TPAQRYMGYATRAYTAYYSGRYTEALDLSEEAVKLYEPGILSELAIYGDDSILMPHLM
                     KSWALWMLGELENSIFQQEAVHAIVETLSSPFAVG"
BASE COUNT          311 a          753 c          735 g          351 t
ORIGIN      
        1 gccctgcgct tcggaattca caccggtccc gcggtcgtgt ggaaccgtcc gggccaggcg
       61 gatcgtctgc aactggggag caccctcgat ctcgccctgg gaatccagag cgcggcgccg
      121 gtcaacggca ttgccgtgag cgcgatgagc cggcggctgc tcgctcgcca tttcgccacc
      181 cgtcccctgc cgccggcccg gcttccgaac gtggacggcc ccgccgaaat ctacgaggcc
      241 ggcgagccgc tggaggggtg gcagcatgaa ggagcccccg tggctcccct ggtcaaccgc
      301 gaggccgaga tccagatttt gctcgaccgc ttccggctgg ccggctcggg cgcggggggc
      361 caagcggttc tgatcgccgg cgagcccggg atcggcaagt cgcgcctgat ccgtgccctg
      421 tccgagcaac tcgccgccga ggcaccgatc tggctgaccg cccagggctc aacgttgacc
      481 cagaacacgc cgctggctcc catcgcccag ctcctgacgc gggcgctctc gggagccgac
      541 gacagcacgg agggctcgga cgaggagagg ttgcgccggc tcgaagaggt gctcgacgag
      601 cacgaccttc cccggccgga atccactgtc ctcctgggcg agctgctcgc ccttccggtg
      661 gcagaccgct acccgccgct cgccctgagc ccggaggcgc ggcgcaagcg gaccctcgcc
      721 gccatcctgg tgttcctcgg cggtctggcc gagcgccgtc ccgtggtggc ggtgatcgag
      781 gatctgcact ggatcgatcc ctcgacactc gagctgctcg acctgctgct gggcgagctc
      841 cccatgctct ctctgctgct cgtggccacg ttccggaccg agttctcggc gacctggcgt
      901 ccgaccgcgg tgacgcagct cagcctgagc ggcctgaatg agtctcagac gaaacagttg
      961 gtggcgcagg tggcggaggg gaaggggttg cccgccgagg tgcaccggga gatcgtggta
     1021 cggacggacg gcaacccgct gttcatcgag gagctgacca aggcggcgct ggaggcggag
     1081 gcgccgctgc acgagccggc cagcgttccg ttgacccttg gagcctcgct cctcgcccgc
     1141 ctcgatcgtc tcggcgacgc caaggaggtg gcgcagctcg cggcggtgct cggccgcacc
     1201 ttctcgctgg agctgctgga ggccctctcc tggatcaagg gggcctccct gtccagcgcg
     1261 ctcgcccggc tcgtccaggc cgagatcctc taccgccaga ggcggccgcg cggggtgggt
     1321 tacatcttca agcacgctct gatccaggac gtcgcctaca tgtcgctcct ggcgagcgac
     1381 cgccagcagc tccaccgccg tctcgtcggc ctgtttcacg aggagtttcc agaggtggcg
     1441 gaggccgagc ccgagctgat ggcgcatcac tgcgagcgcg gcggcctggt ggtggagtcg
     1501 gtcgactacc tgctaggagc cggcgtgcgg gccgcacagc gttcggccta tctggaggcg
     1561 atgagccatc tgaaccgggc tctggacctg ctgtacagtc tgcccctggc cgcgacgccc
     1621 gctacagcag ccgagctgct ggagcgggag ctctcggtgc ggtccatgct catcgtcgtc
     1681 ctcgaagcgg tcaaaggttg gggagccccc gaggtcgccg ccaatgcgga gcggtgcgcg
     1741 gtcctctgcc gtgaagtcgg agatcacgcg agcctgatcc ccgcgctcag ctcgctctgg
     1801 gcgcatcacc ttctgcgcgg ggaccgccaa cccactgtcg atctcgcgga caggatcgcc
     1861 cgtctggcgg agacccccgc ccagcgctat atgggctacg cgacccgggc ctacaccgcc
     1921 tactacagcg gccggtacac ggaagcgctg gatctgtccg aagaggcggt gaagctgtac
     1981 gagcccggca tcctttccga gctggcgatc tacggggacg attccatcct catgccgcac
     2041 ctcatgaagt cgtgggcgct gtggatgctc ggcgagctgg agaactcaat cttccagcag
     2101 gaggcggtcc atgcgatcgt cgagaccttg tcctcgccct tcgccgtcgg
//
LOCUS       DASPJI010000049         2750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_174592, whole genome shotgun sequence.
ACCESSION   DASPJI010000049 DASPJI010000000
VERSION     DASPJI010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174592"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VEL74_01320"
     CDS             complement(<1..1146)
                     /locus_tag="VEL74_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HYG61193.1"
                     /translation="MPETMPELWELSAEETARRVSAREVSAVEVTRAALERTDRVEPL
                     IAAYLEVFASEALARAAEVDRRLGAGEEPGPLAGVPVALKDNLSLAGHRLTCASKILC
                     GYVAPYTATAVERLLAAGAVPIGRANMDEFAMGSSCENSAFQTTRNPWDLTTVPGGSS
                     GGAAAAVAAGSVPLGFGSDTGGSVRQPAALCGVVGVKPTYGRVSRYGLVAFASSTDQI
                     GPMARTVRDAALATRVIAGADPNDSTCAERPVDDYLAGIEDGIEGLRVGVIKEVEHAG
                     LPAGVTASWRETLGRLEAAGARIVEVSVPSLDAAVAIYYVIATCEASANLARFDGVRY
                     GRRAAGAKSLLDVYAGSRSEGFGPEVKRRIMLGTFALSSGYYEAYYGR"
     gene            complement(1139..1438)
                     /gene="gatC"
                     /locus_tag="VEL74_01325"
     CDS             complement(1139..1438)
                     /gene="gatC"
                     /locus_tag="VEL74_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HYG61194.1"
                     /translation="MALTPEHVKKIATLARLRFSPDEEAKLTGQLGGIVDYIDQLQRY
                     DLPEVGEPAGGGPAAPEADDSARPCLPREQLLANAPAAMDGFLLVPEVKGGGEDA"
     gene            1505..>2750
                     /locus_tag="VEL74_01330"
     CDS             1505..>2750
                     /locus_tag="VEL74_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61195.1"
                     /translation="MSLRAPRRAFQILARQPAVAALLDVCGPTECHLVGGVLRDRALG
                     LPVHDIDVVVAGRGREICERLAATLPARLVLLGGKEFAAFRLVGGDFVLDLWDREAMP
                     LHDDLARRDFTVNAFAYDPRAGQPGDAGEVIDPFGGIGDLHRRVLRATTAQSFTGDPL
                     RVLRLPRLLLQLPGFSTEPDTVALARQSAPRLADVAAERIRDELWNLFGYSEAHRGLT
                     LLMELDLYPGLWLGRPGEPGPPGTALAELEALPGCLRELREQEPSLAAAVNLRSARFA
                     ALFGSLPHPGDALRAFRDAGSLTRQAAADAGLLLDSGPLPDDEVARRRFLYKVGHLWP
                     AAVCYQGARAAAHGQIANWRRSLPPLLDLLRREGETLLNPPRLLTGEEVQALLGIQEG
                     PAVGRALAAVRQAQVDGAVRTRE"
BASE COUNT          338 a         1048 c          987 g          377 t
ORIGIN      
        1 gcggccgtaa taagcctcat agtagccgga cgagagggcg aacgtcccca gcatgatgcg
       61 gcgcttgacc tcggggccga acccctcgct gcggctgccg gcgtagacgt cgagcaggct
      121 cttcgccccc gccgcgcggc gcccatagcg gacgccgtcg aagcgcgcca ggttggcgct
      181 cgcctcgcag gtggcgatca cgtagtagat ggcgacggcg gcgtcgaggc tgggcaccga
      241 cacctcgacg atccgcgccc ccgccgcctc cagccgcccc agggtctcgc gccagctcgc
      301 ggtgacgccc gccggcagcc cggcgtgctc gacctccttg atcacgccga cgcgcagccc
      361 ctcgatgccg tcctcgatgc cggcgaggta gtcgtccacc ggccgctcgg cgcaggtgga
      421 gtcgttcggg tcggcgccgg cgatgacgcg ggtggcgagc gccgcatcgc gcacggtgcg
      481 ggccatgggt ccgatctggt cggtcgagga ggcgaaggcg accaggccgt agcgcgacac
      541 ccggccgtag gtgggcttga ccccgaccac gccgcacagc gccgccggct ggcgcaccga
      601 gccgccggtg tcggagccga agccgagcgg cacgctgccg gcggccaccg ccgcggcggc
      661 gccgccgctc gagcctcccg gcacggtggt caggtcccag gggttgcggg tggtctggaa
      721 cgccgagttc tcgcacgacg agcccatggc gaactcgtcc atgttggccc gcccgatcgg
      781 gacggccccc gcggcgagca ggcgctccac ggccgtggcg gtgtacgggg ccacgtagcc
      841 gcacaggatc ttcgacgcgc aggtcagccg gtggccggcg aggctcaggt tgtccttgag
      901 cgccacgggc acgccggcga gcggccccgg ctcctcgccg gcgccgagcc gccggtcgac
      961 ctcggccgcc cgcgccaggg cctcggaggc gaagacctcc agataggcgg cgatcagcgg
     1021 ctcgacccgg tcggtgcgtt ccagcgccgc gcgggtgacc tcgacggccg agacctcgcg
     1081 ggcgctgacg cgccgggcgg tctcctcggc ggacagctcc cagagctcgg gcattgtctc
     1141 aggcatcttc gccgcccccc ttcacctcgg gcacgaggag gaagccgtcc atggcggcgg
     1201 gggcgttggc gaggagctgc tcccggggga ggcaggggcg ggcgctgtcg tccgcttcgg
     1261 gggccgcggg cccgccgccg gcgggctccc ccacctccgg caagtcgtac ctctggagct
     1321 ggtcgatgta gtcgacgatc ccgccaagct ggccggtgag cttggcctcc tcgtccggag
     1381 agaaacggag ccgggccagg gtggcgattt tctttacatg ttcgggggtt agtgccatgg
     1441 ccttcaagta ggagtttagc cttggcgcca tgtcaaggca atcatttcct tagaatgggc
     1501 aggcatgagc ctccgcgcac cccgccgcgc cttccagatc ctcgcccgcc agcccgcggt
     1561 cgccgcgctc ctggacgtct gcggcccgac cgagtgccac ctggtcggag gggtgctccg
     1621 cgaccgcgcc ctgggcctgc cggtgcacga catcgacgtg gtggtggcgg gccgcggacg
     1681 ggagatctgc gagcgtctgg cggccacgct ccccgcccgg ctggtgctcc tcgggggcaa
     1741 ggagttcgcc gccttccgtc tggtcggggg ggatttcgtc ctcgacctct gggaccggga
     1801 ggcgatgccg ctccacgacg acctggcgcg ccgggatttt accgtcaacg ccttcgccta
     1861 cgatccccgc gccggccagc cgggagacgc cggcgaggtg atcgacccct tcggcggcat
     1921 cggcgacctc caccgccggg tgctgcgggc cacaaccgcc cagagcttca ccggcgatcc
     1981 gctgcgggtg ctgcgtctcc cccgcctgct cctacaactg cccggcttct ccaccgagcc
     2041 ggacacggtc gccctggcgc gccagtcggc cccgcgcctg gcggacgtcg ccgccgagcg
     2101 gatccgggac gagctctgga acctcttcgg atactccgag gcccaccgcg gcctcacgct
     2161 gctgatggag ctggacctct atcccggcct ctggctcggc cgccccggcg agcccggacc
     2221 gccggggacc gccctggcgg agctggaagc cctgccgggc tgcctgcggg agctccggga
     2281 gcaggagcca tcactcgccg ccgccgtcaa cctccgaagc gcccgcttcg ccgccctctt
     2341 cggcagcctg ccgcatcccg gggacgccct gcgcgccttc cgggacgccg gctctctcac
     2401 ccgccaggcc gccgcggacg ccgggctcct cctggactcg gggccgctgc ccgacgacga
     2461 agtggcaagg cgccgcttcc tctacaaggt ggggcatctc tggcccgccg ccgtctgcta
     2521 ccagggggcc cgcgccgccg cccacgggca gatcgcgaat tggcgccgga gcctccctcc
     2581 cctcctcgac ctccttcgcc gggaggggga gaccctcctc aacccgccgc gcctcctcac
     2641 gggagaggag gtgcaggcgc tcctggggat tcaagagggc cccgccgtcg gccgcgccct
     2701 cgccgccgtc cggcaggccc aggtggacgg ggcggtccgg acgcgggagg
//
LOCUS       DASPJI010000050         4737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_291274, whole genome shotgun sequence.
ACCESSION   DASPJI010000050 DASPJI010000000
VERSION     DASPJI010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291274"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4737)
                     /locus_tag="VEL74_01335"
     CDS             complement(<1..>4737)
                     /locus_tag="VEL74_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61196.1"
                     /translation="YLPLDPYYPRERLAYMLADGAPAALLVQDRFRDRLPELGAVPGL
                     SLEDELAGVAAEPGDPPPAAPAFPDQAAYVLYTSGSTGRPKGVVVSHGGLAGMHLAWK
                     PVWPAAPGPLVQLQAASFSFDVFGEEVLRALGSGGSLLLCPQELLGDPERLVGLMRRE
                     GVTIADLVPAVARGVARHLETTGERLDSLSLLVIGADVFQVEERERFVPLCGPGTRLV
                     GAYGVTEASVDSTWFERTAVPLPAGTPVPLGRPFEGTRVHLVDPGFRPVPLGAAGELT
                     LGGSGLARGYLGRPDLTAERFVPDPFGRSGERLYRTGDRARWLPDGNLQFLGRIDQQV
                     KIRGVRLETGEIESALAACPGVRAAAVVATGAVASRTLTAFLVLEPDTAPSPPALRAH
                     LRERLPEAMVPAAFVALDALPLTPNGKVDRRALAQLAPAAAVHASSPADTLPGERTPF
                     EDVLAAIWCEVLGVDRVGPEDDFFDLGGHSLLATQVISRVRTSLGVELPVRALFETPR
                     LAALARAVETALRSREGSRPPLRPAPRDGGPLPLSFAQERLWFIEQLQPGTPTYNVVI
                     AAVLDGRLDAGALARGLATIVERHEALRTRFPISGGADGSPVQVIDPPPAWVLPQVDL
                     SGLPAGSREAAAQALLDGESRRPFDLAAGPLLRAVLLRLSPEEHVALIAAHHIVVDGW
                     SMQVLTRELTALYAAEVVGEPSPLAPLPIQYADFAHWQRQWLSGEALASQLAFWRERL
                     AGAPAVLDLPLDRPRPALQSFRGARQELTLDSAVAAALRGLARRTGSTLFMTLLAAFD
                     VLLQRWSGATDLVVGTPSANRDREEIEGLIGFFVNTLALRADLAGDPAFPELLGRVRE
                     ATLAAYTHQDLPFEKIVAELKPERDLAYTPVFQVMFLLQAMNAGTLGDVELPGLTMRF
                     PEVETETTKFDLIVSFDESPAGLGGEWRYNVDLFDGTTMRRMTRHFAVLLAGIAADPE
                     RPLPELPWITEAERHQTVTEWNGEWNDPSSASTEACFPDLFRERMEAWPDAVAVAWQD
                     VHLSYADLDRRSEAAASLLVERGVSPDSVVGLLARRGSELPVAILAIQKAGGAWMPLE
                     PAQPRARLEKMIEGSGAGLLLTGEGIEPPAVVPALPLADLLAAPPAGGRLPSRLQSGN
                     IAYLLYTSGSTGTPKGVMVTHRGLREHMLSKAAYLGLTAGDVVAQTAAPTFDISVWQL
                     LSVLLAGGRVQMLATDEAREPARLLAAAEREGITVLQLVPSLLRAVCEVAEESPDRPG
                     LAALRWLIPTGEALPPDLGRRWLALYPGIPMVDAYGPTECTDEVSHQILREALRPERT
                     VTPIGRPSRGIRLHVVDRQLRPVPIGVTGELCVAGVAVGRGYRYDPARTAEAFVPDPF
                     GREAGEPGSRLYRTGDLARLLPDGALEYLGRRDRQVKIRGFRIETGEVEAALRAHPEV
                     REAAVLARPVPGSTDLRLVAYVVPGGGGDAWTSGLRTFLADRLPDSMIPAAFVALDSL
                     PLSANGKLDVRALPEPEQVTERAAAEPPGTPLEREIAALWQEVLGTDRFGVRDNFFEL
                     GGDSIKGAILMHRLQRRLNVVLSVVT"
BASE COUNT          671 a         1684 c         1839 g          543 t
ORIGIN      
        1 gggtgaccac ggagagcacc acgttgaggc ggcgctgcag ccggtgcatc aggatggcgc
       61 ccttgatgga gtcgcccccc agctcgaaga agttgtcgcg gacgccgaag cggtccgtgc
      121 ccagcacctc ctgccagagc gcggcgatct cccgttcgag cggggtgccc ggcggctcgg
      181 cggcggccct ctccgtcacc tgctccggct ccggcagggc gcggacgtcg agcttgccgt
      241 tggccgacag gggcagagag tcgagcgcca cgaaggcggc cgggatcatg gagtccggca
      301 gccggtcggc caggaaggta cgcaggccgg acgtccaggc gtctcccccg cctccgggca
      361 ccacgtaggc gacgagccgc aggtcggtgc ttcccggaac cgggcgcgcc agcacggcgg
      421 cctcccgtac ctccgggtgg gcgcgcagcg ccgcctcgac ctcgccggtc tcgatccgga
      481 agccgcggat cttcacctgg cggtcgcgac gcccgaggta ctccagcgcg ccgtcgggca
      541 gcagacgggc gaggtcgccg gtgcggtaca ggcgcgagcc cggctccccc gcctcccggc
      601 cgaacggatc gggcacgaag gcctcggctg tgcgcgccgg gtcgtaccgg tagccccgcc
      661 ccaccgccac gccggcgacg cacagctcgc ccgtgacgcc gatgggcacg ggccggagct
      721 ggcgatccac cacgtgcagc cggatgccgc gcgacggccg gccgatgggc gtgaccgtcc
      781 gctccggtcg cagggcctcg cgcaggatct ggtgggagac ctcgtccgtg cactcggtgg
      841 ggccgtaggc gtcgaccatg gggatgcccg ggtatagggc gagccaccgc cggcccaggt
      901 cggggggcag cgcctcgccc gtggggatca gccagcgaag cgccgcgagg cccgggcggt
      961 ccggcgactc ctcggccacc tcgcagaccg cgcggagcag ggacggcacg agttggagga
     1021 cggtgatccc ctcgcgctcg gccgccgcca ggagacgcgc cggctctcgt gcctcgtccg
     1081 tggcgagcat ctggacccga ccgccggcga gcaggaccga caggagctgc cagaccgaga
     1141 tgtcgaacgt cggcgcggcg gtctgggcga ccacgtcgcc cgcggtcaac ccgagatagg
     1201 cagccttgga cagcatgtgc tcgcgcaggc cgcggtgggt caccatcacc cccttcggcg
     1261 tgccggtgga gcccgacgta tagagaaggt aggcgatgtt cccggactgg agccgggacg
     1321 gcaggcgccc tccggccggt ggcgcggcga ggaggtccgc caggggcagc gccgggacaa
     1381 ccgcgggggg ctcgatcccc tcccccgtca ggagcaatcc ggcgccgctc ccttcgatca
     1441 tcttctccag gcgcgcgcga ggctgggccg gctcgagggg catccaggcg ccccccgcct
     1501 tctggatggc caggatcgct accggcagct cggaaccgcg gcgggcgagc aggccgacga
     1561 cggagtccgg gctgacgccg cgctccacca gcagcgaggc cgcggcctcg gagcggcggt
     1621 cgagatcggc atagctcagg tggacgtcct gccaggcgac cgccaccgcg tccggccagg
     1681 cctccatcct ctctctgaag agatccggga agcaggcctc cgtggaggcg ctcgacgggt
     1741 cgttccactc gccattccat tcggtgaccg tctgatggcg ctcggcctcg gtgatccagg
     1801 gcagctccgg caggggccgc tccggatcgg cggcgatccc cgcgaggagg acggcgaagt
     1861 ggcgggtcat gcgccgcatc gtggtgccgt cgaacaggtc gacgttgtag cgccactcgc
     1921 cgcccagccc cgcgggggac tcgtcgaagc tcacgatgag gtcgaacttc gtggtctccg
     1981 tctccacctc ggggaagcgc atggtcaggc cgggcagctc gacgtcgccg agagttccgg
     2041 cgttcatcgc ctggagcagg aacatcacct ggaagaccgg cgtgtaggcg aggtcgcgct
     2101 cgggcttgag ctcggcgacg atcttttcga acggcaggtc ctgatgagtg taggcggcga
     2161 gggtcgcctc ccgcaccctc ccgaggagct ccgggaaggc cggatcgccc gccaggtccg
     2221 cccgcagcgc cagggtgttg acgaagaagc cgatcagccc ttcgatctcc tcgcggtcgc
     2281 ggttggccga gggggtgccg acgacgaggt cggtcgcgcc gctccagcgc tggagcagca
     2341 cgtcgaaggc ggcgagcagg gtcatgaaga gagtggagcc ggtgcgcctg gcgagccccc
     2401 gcagcgccgc ggcgacggcg ctgtccaggg tgagctcctg gcgggcgccg cggaacgact
     2461 gcagggccgg acgcggccgg tcgagcggca ggtcgagcac cgccggggcg ccggcgaggc
     2521 gctcgcgcca gaaggcgagc tgggaggcga gggcctcgcc gctcagccac tggcgctgcc
     2581 agtgcgcgaa gtcggcgtac tggatcggca acggtgccag cggcgagggc tcccctacca
     2641 cctccgccgc gtagagggcc gtcagctcgc gggtcagcac ctgcatggac cagccgtcca
     2701 ccacgatgtg atgggcggcg atcagggcga cgtgctcttc agggctcagc cgcagcagca
     2761 cggcgcgcag cagcgggccg gccgccaggt cgaacggtcg ccgcgactct ccgtcgagca
     2821 gcgcctgggc ggcggcctcg cgggatccgg ccggcagccc ggagaggtcg acctgaggca
     2881 gcacccaggc cggcggcgga tcgatcacct ggacgggcga gccgtcggcg ccgccggaga
     2941 tcgggaagcg cgtgcgcagc gcctcgtggc gctccacgat cgtggcgagg ccgcgggcga
     3001 gcgccccggc atcgagccgg ccgtccagca cggcggcgat caccacgttg tacgtcggtg
     3061 tgccgggctg gagctgctcg atgaaccaga ggcgctcctg ggcgaaggag agcggcagcg
     3121 ggccgccatc ccgcggcgcc ggccggagcg gcggccggga tccctcgcgc gagcgcagcg
     3181 ccgtctcgac cgcgcgggcc agggcggcga gcctgggcgt ctcgaacagc gcccgcaccg
     3241 gcagctccac gcccagggag gtgcggacgc gcgagatcac ctgcgtcgcg aggagcgagt
     3301 ggccgccgag gtcgaagaag tcgtcctcgg gaccgacgcg gtccacgccc agcacctcgc
     3361 accagatggc ggcgagcacg tcttcgaacg gcgtgcgctc tccggggagg gtgtcggccg
     3421 ggctcgaggc gtggacggcc gccgccggcg cgagctgggc cagcgcccgg cggtccacct
     3481 tgccgttcgg ggtgagcggc agggcgtcga gcgcgacgaa ggcggcgggc accatggctt
     3541 cggggagccg ctcgcggagg tgggcgcgga gggccggcgg ggacggtgcc gtgtccggct
     3601 ccagcaccag gaaggcggtc agcgtccgcg aagcgacggc accggtcgcc acgaccgcgg
     3661 cggcgcgcac gccggggcag gcggcgaggg cggactcgat ctcgccggtc tccagccgca
     3721 cgccgcggat cttcacctgc tggtcgatgc ggccgaggaa ctggaggttg ccgtcgggca
     3781 gccagcgcgc ccggtcgccg gtccggtaga ggcgctcgcc cgagcgtccg aacgggtcgg
     3841 ggacgaacct ctcggcggtg aggtcgggac ggccgagata gccgcgggcg aggcccgagc
     3901 cgccgagggt gagctcgccg gccgcgccca gcggcaccgg ccggaacccg gggtcgacga
     3961 ggtggacccg cgtgccctcg aacgggcgcc cgagcggcac cggcgtgccc gccggcagcg
     4021 gcacggcggt gcgctcgaac caggtggagt cgacgctcgc ctcggtgacg ccataggcgc
     4081 cgacgagccg ggtgcccggt ccgcacagcg ggacgaagcg ctccctctcc tccacctgga
     4141 agacgtcggc gccgatgacc agaaggctca gggagtcgag gcgctcccct gtcgtctcca
     4201 gatggcgggc gacgccgcgg gccacggcgg ggacgaggtc ggcgatggtc accccctcgc
     4261 gccgcatgag accgacgagc cgctcgggat cgcccagcag ctcctgcggg cagaggagga
     4321 ggctgccgcc gctgccgagc gcccgcagca cctcctcgcc gaagacgtcg aacgagaagc
     4381 tcgccgcctg gagctggacg agcggcccgg gagccgccgg ccagacgggc ttccaggcca
     4441 ggtgcatgcc ggccaggccg ccgtggctca ccacgacgcc cttgggccgc ccggtcgagc
     4501 ccgaggtgta gagcacgtag gcggcctgat cggggaacgc cggcgcggcc ggcgggggat
     4561 cgcctggctc ggccgcgacg ccggcaagct cgtcctccag gctcagcccc ggaaccgcgc
     4621 cgagctccgg caggcggtca cggaaccggt cctggaccag cagcgccgcc ggagcgccgt
     4681 ccgcgagcat gtaggccagg cgctcgcgcg ggtaataagg atcgagcggc aggtagg
//
LOCUS       DASPJI010000051         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_234115, whole genome shotgun sequence.
ACCESSION   DASPJI010000051 DASPJI010000000
VERSION     DASPJI010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234115"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /locus_tag="VEL74_01340"
     CDS             <1..1493
                     /locus_tag="VEL74_01340"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYG61197.1"
                     /translation="RQAPRHLDRLRTEGDTLFRALFQGEVRSRFDESLGAVQAFPGRG
                     LRLRLRFDLRDPGLAALYDLPWELLYRRETGDFLSLRPATPVIRHLDAARPNRSEAWP
                     ATWRILVVASSAGTRRLNLRREIHNLRQALRGQSHVEAVFLQPATVEAFYQALRREPF
                     HVLHFMGHGVLDAAAEEGSLVFEDEAGGAQRLRGEVLATMVSPFRDLRLVVLNACHGA
                     DAAGEGRAFTGVATALGMSGIPAVLAMRSRISDRAAVAFSKAFYSDLVAGATVEQATT
                     EGRRAVYLLEESGAEWATPVLFQRGDQPQSYPGLTEGERHVSPGAPVRAPEAPPAQGS
                     GLSEGPGCLRLSGFILLNLSVSITYLFFHTLTALGIIINSKPYRDSWLALQQEMDAYA
                     NPIEPWDLTVLLGFSCGYILLFVLSVKMGLRKGLTSGIYAAILGTFAVTLLNTFWQMM
                     FFKESLVQTPWLISGFSTTVQMMFFTWLLDLALKKARSSFRRRSGD"
     gene            1587..>2381
                     /locus_tag="VEL74_01345"
     CDS             1587..>2381
                     /locus_tag="VEL74_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYG61198.1"
                     /translation="MSSSIPRESISHPAVPTVLWRLLLLCACLQLLGCATTTATTNRD
                     RVVVLLGASSGFGKGVALELAEQGANVVVAARRTELLEELARECEQRGGRALAVTTDA
                     SDEGEVERLAEAAVAQFGRIDVWINLAGIGAFGRFEEIPLADHHRLLDVNVKGVINGS
                     YFAMRRFRQQGAGTLINISSVAGRVGFPYYPSYSASKHAVTGLGVALNQELRLNGDRN
                     IHVSTISPYASNTPWFEHAANYLGHSPSMVLMDPPGKIVDAIVKATL"
BASE COUNT          360 a          866 c          753 g          402 t
ORIGIN      
        1 ccaggcaggc gccccggcac ctcgaccgcc tacggactga gggggatacc ctcttccgcg
       61 cgctcttcca gggggaggtt cgaagccgtt tcgacgagag cctcggagcg gtccaggcgt
      121 ttccgggccg cggcctgcgg ctccgtctcc gcttcgatct cagggatcct ggcctcgccg
      181 ccctctatga cctgccctgg gagctcctct accggcgcga gaccggcgac ttcctgagcc
      241 tccggcccgc gacgccggtc atccgccacc tcgacgcggc gcgtcccaac cggtccgaag
      301 catggccggc gacctggcgc atcctggtcg tcgccagctc cgccggaacg cgccgcctga
      361 atctccggcg cgagatccat aacctgcgtc aggccctgcg gggccagagc cacgtcgagg
      421 cggtcttctt gcagccggcg acggtagagg ccttctatca ggccctacgc cgggaaccgt
      481 tccatgtcct gcacttcatg gggcacggcg tgctggatgc ggctgcggag gaaggatcgc
      541 tggtcttcga agacgaagcc ggcggagccc aaaggctccg cggcgaggtc ctggcgacga
      601 tggtgagccc gttccgggac ctgcggctcg ttgtcctcaa cgcctgccac ggcgccgacg
      661 cggccggcga gggccgggcc ttcaccggcg tcgccaccgc cctcgggatg agcggcatcc
      721 ccgccgtact ggcgatgcgc tcccggatca gcgaccgggc cgccgtggct ttcagcaagg
      781 ctttctacag cgacctggta gccggtgcca cggtggaaca ggcgaccacc gaaggacggc
      841 gcgccgtcta tctcctcgag gagtccggag cggagtgggc gacccccgtc ctcttccagc
      901 ggggagacca gccgcaatcg taccccggcc tcacagaggg cgaacggcac gtctcccctg
      961 gcgctccggt ccgggccccc gaggcacctc ccgcccaggg ctccggcctc tccgaaggcc
     1021 ccggctgcct gcggctctcg ggctttatcc tgctcaacct gtcggtttcg atcacctatc
     1081 ttttcttcca tacgctcacc gcgctaggga tcatcatcaa ctccaagccc taccgcgata
     1141 gctggctggc gctccagcag gagatggacg cctacgcgaa cccaatcgag ccgtgggatc
     1201 tcacggtcct gctcggcttt tcatgtggct atatcctgct cttcgtcctg tccgtcaaaa
     1261 tggggctccg gaagggcctg accagcggga tctacgctgc cattctgggc acgttcgccg
     1321 tcacgctgct aaacaccttc tggcagatga tgttcttcaa ggagtcgctc gtccagacgc
     1381 cctggctgat cagcggcttc agcacgacgg tccagatgat gttcttcaca tggctgctgg
     1441 accttgcgct caagaaggcg agatccagct tccggcgccg gtccggcgac tgaatcgtcc
     1501 ggcggcgccg gggcacagcc cttgcttcaa aatcccttcc acatctcagg accggccggc
     1561 cctgcgatcc cttcctggag gtctccgtga gctcatccat tcccagagag agcatttccc
     1621 accctgcagt ccccacggtt ctctggcggc tcctgctgct ctgcgcctgt ctccagctcc
     1681 tcggctgcgc gacgaccacc gcgacgacga accgcgaccg ggtcgtcgtc ctcctcggcg
     1741 cctccagcgg cttcgggaaa ggggtcgcgc tcgagctcgc ggagcagggg gcgaacgtcg
     1801 tggtcgcggc gcggcggacc gagctcctcg aagagctggc gcgcgagtgc gagcagcgag
     1861 gcggccgggc gctcgcggtc accacggacg ccagcgacga gggcgaggtc gagcggctcg
     1921 ccgaggccgc ggtcgcccaa ttcggccgca tcgacgtctg gatcaacctg gcgggcatcg
     1981 gcgccttcgg gcgcttcgaa gagatcccgc tcgccgatca ccaccgcctc ctcgacgtca
     2041 acgtcaaggg ggtgatcaac ggcagctact tcgcgatgcg ccggttccgg cagcaggggg
     2101 ccggcacgct catcaacatc tcctccgtcg ccgggagggt cggcttcccc tattacccga
     2161 gctactccgc gtccaagcac gcggtgaccg gcctcggcgt cgccttgaac caggagctgc
     2221 ggctgaacgg cgaccggaac atccacgtct ccacgatctc gccgtacgcg tcgaacaccc
     2281 cgtggttcga gcacgcggcg aactatctcg gccacagccc gtcgatggtc ctgatggatc
     2341 cccccgggaa gatcgtcgac gcgatcgtca aggcgacgct g
//
LOCUS       DASPJI010000052         6899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_174934, whole genome shotgun sequence.
ACCESSION   DASPJI010000052 DASPJI010000000
VERSION     DASPJI010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6899)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174934"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VEL74_01350"
     CDS             <1..578
                     /locus_tag="VEL74_01350"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HYG61199.1"
                     /translation="LVSVSVIGLTIIGLHNSAYGPIGDARLPAPQGTLMAVIIKGVLS
                     QDLPWGFIFVGAALAVVVQLCGVSGLAWAVGAYLPISTTFPIFIGGVIRWLSDRIRKQ
                     PAAEESELSSGMLFSTGLVAGGAISGLLVAVLISMEAGGALARIRDAAASVGAGLNLG
                     IFGAGGEWSALFMFAILCVILLRRSLRKLEI"
     gene            complement(622..1803)
                     /locus_tag="VEL74_01355"
     CDS             complement(622..1803)
                     /locus_tag="VEL74_01355"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HYG61200.1"
                     /translation="MRTAGARKSSVKKSPPEKRADEVVAGALPAEALEGDFPAIDLPV
                     HPPFPPMEAKSVDAIPSAAPGDGGWLYEPKWDGFRCLAFRKGEQVLLQSKSCQPLGRY
                     FPELVEALRALPVKTFVLDSEIVISREGKLSFDDLLQRIHPAESRIRKLARETPASLF
                     AFDLLVDARGTSLVDLPLADRRERLEKLFGKLGEPESVVLSPATDERELAEQWIRDLG
                     AGGLDGVIAKRSGEPYLSGERTGMVKIKRHRTADCVVGGFRWAHKGEKKIGSILLGLY
                     DGQGNLDHVGFSSSFTDTERRELEEILVPLIEPPGFTGKAPGGPSRWSQGRSTEWEPL
                     RPALVCEIRYDHFSGGRFRHGTKFLRWRPDKEPRACTYQQLERGKGDWKKGLKSLGIE
                     V"
     gene            complement(1867..2250)
                     /gene="apaG"
                     /locus_tag="VEL74_01360"
     CDS             complement(1867..2250)
                     /gene="apaG"
                     /locus_tag="VEL74_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HYG61201.1"
                     /translation="MSDTTTRGIRIQVHSFYDEDRSAPHESYYFFAYHVRITNEGSVT
                     AQLMSREWIITDGNGEVQRVQGPGVVGEQPVLAPGESYEYTSFCPLATSVGSMEGSFF
                     MVPVGGEGFKAVIAPFVLAVPHAVN"
     gene            2388..3179
                     /locus_tag="VEL74_01365"
     CDS             2388..3179
                     /locus_tag="VEL74_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HYG61202.1"
                     /translation="MEWISNPESWIALATLTALEIVLGIDNVIFISILAGKLPQEQQA
                     KARRVGLMLAMGIRLLLLASLAWMARLTSPLFTVWNQEISGRDLILLLGGLFLLGKAT
                     HEIHDKLEGEEGHASAKVVASFTAVIVQILLLDIVFSLDSVITAIGMANELAVMMIAV
                     VIAVLVMLLSAGKISDFVGRHPTVKMLALSFLLLIGMSLIAEGFDQHIPKGYIYFAMG
                     FSVFVEMLNLRAKKVTGEAPVQLHQRYAPEAPKAPKAPAKGRGRK"
     gene            complement(3235..4923)
                     /locus_tag="VEL74_01370"
     CDS             complement(3235..4923)
                     /locus_tag="VEL74_01370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HYG61203.1"
                     /translation="MAESIQTTLPNLRRTLRRTFGFRSLREGQEEVIHSVMEGRDTLA
                     IMPTGAGKSLCYQLPGKELPGATVVVSPLLSLMKDQVDKLQELGIDAAEVNSTLTARE
                     EGEVLEEIRQEQRDFVFVTPERVTQPEFLETLRKNRIDVFVIDEAHCISQWGHDFRPS
                     YLHLREVIEALGNPPVLALTATATEQVIDDIRRMLGREEMEVIATGVYRDNLHLEVIP
                     VGNDEIKRTHLARLLGEMEGTGIVYCSTVRDCEEVTDHLTSLGFDVARYHGRLGTRER
                     RSTQDRFMSGQLKAIIATNAFGMGIDKPDIRFVIHYNLPGSLESYYQEAGRAGRDGER
                     ARCVLLYLAEDRNTQIFFLNGRYPKREHFDAVYQALERLKADRREVSAGDVQERAGGV
                     AKAKVRVVLATMKDLELVEETEPGHFKLLRGGLSGEELAAMGKRYEQRAEGDRDRLRR
                     MVLYCQTALCRWKAIVDYFGETAEWERCANCDGCDRPFTEPVVPRAEPPVRVPSRVEV
                     LPLPPLLGGGKPGDLQQGDVLTLAMYGRAEVRAVQGDHLVLEFSDGETREFRLG"
     gene            complement(5480..6571)
                     /locus_tag="VEL74_01375"
     CDS             complement(5480..6571)
                     /locus_tag="VEL74_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYG61204.1"
                     /translation="MTTPFGKHRGLLVRQEDPFNGGPDLPLLRRSFLTPNDLFFVRNH
                     GAVPEVDASSYRLVVGGRVQRPLRFSLEELRRLPRMTVDATLQCAGNRRLEMAAVAPI
                     PGELGWGAEAISTAKWGGVALRDLLAAVAPEPGAGHVAFTGLDETERHGHRFCFGGSI
                     PLEKAMSSEVLLAYEMNGAPLPPVHGAPLRAVVPGYIGARSVKWLSAITVQDEPSGNY
                     FQAKAYRLFGPQVSAQDVDWESGLMLGELPVNSVICAPAEGETVPAGEVVVQGYAMAG
                     GGRSVARVDLSLDGGATWRTAGLGGEQGRWAWRLWEARLELPAGEHEIVCRAWDSAAQ
                     TQPEHPAQVWNFKGYANNAWGRVRITCTG"
     gene            complement(6579..>6899)
                     /locus_tag="VEL74_01380"
     CDS             complement(6579..>6899)
                     /locus_tag="VEL74_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase"
                     /protein_id="HYG61205.1"
                     /translation="ALHDHAGIWCVEGVVQGEMEVSRYELLEEQEDGVCRFAARGRAL
                     AVAGSAGALIPPFEHHVLANLQPDRTSLTLHVYGGEMDHCTIFEPLAGGLYQKRLRPL
                     TYDD"
BASE COUNT         1013 a         2415 c         2377 g         1094 t
ORIGIN      
        1 tcctcgtctc ggtgtcggtg atcggcctga ccatcatcgg cctgcacaac tcggcctacg
       61 gcccgatcgg cgacgcccgc ctgcccgccc cgcagggcac gctgatggcg gtcatcatca
      121 agggcgtgct gtcgcaggac ctcccctggg gcttcatctt cgtcggcgcg gccctggcgg
      181 tggtggtgca gctctgcggc gtctccggcc tggcctgggc ggtcggcgcc tacctgccga
      241 tctcgaccac cttcccgatc ttcatcggcg gcgtgatccg ctggctctcc gaccgcatcc
      301 gcaagcagcc cgcggccgag gagtcggagc tgtcgtccgg catgctcttc tccaccggct
      361 tggtggcggg aggcgccatc tcgggcctgc tcgtcgcggt gctgatctcc atggaggcgg
      421 ggggagcgct ggcgcggata cgggacgccg ccgcttcggt gggcgccggc ctcaatctgg
      481 ggatcttcgg cgcgggaggg gagtggagcg ccctcttcat gttcgccatc ctctgcgtga
      541 tcctgctccg ccgctcgctg cgcaagctcg agatctgagc cggtcttccc gggggggctc
      601 ccttcggagc cctcccgccg tctacacctc gatccccagc gacttcagac ccttcttcca
      661 gtcgcccttt ccgcgctcga gctgctggta ggtgcaggcc cgcggctcct tgtccgggcg
      721 ccagcgcagg aacttggtgc cgtgccggaa gcggccgccc gagaagtggt cgtagcggat
      781 ctcgcagacc agcgccggcc gcagcggctc ccactcggtg gaccgcccct ggctccaacg
      841 gctgggaccg cccggcgcct tgccggtgaa accggggggc tcgatcagcg gcacgaggat
      901 ctcctcgagc tcgcggcgct cggtgtcggt gaagctggag gagaagccga cgtggtcgag
      961 gttcccctgg ccgtcataga ggccgagcag gatcgacccg atcttcttct cccccttgtg
     1021 ggcccagcgg aagccgccga ccacgcagtc cgcggtgcga tggcgcttga tcttgaccat
     1081 gccggtccgc tcgcccgaga ggtacggctc gccggaccgc ttggcgatca ccccgtcgag
     1141 gcccccggcc ccgaggtcgc ggatccactg ctcggccagc tcccgctcgt ccgtggcggg
     1201 cgagagcacc accgattcgg gctccccgag cttgccgaag agcttctcca gacgctcgcg
     1261 gcggtccgcc agcggcaagt ccaccagcga cgtcccccgc gcgtccacca gcagatcgaa
     1321 ggcgaagagg ctcgccggcg tctcccgcgc cagcttgcgg atgcggctct ccgccgggtg
     1381 gatgcgctgc aagaggtcgt cgaacgagag cttcccctcg cgcgagatga cgatctcgct
     1441 gtcgaggacg aaggtcttga ccggcagggc gcggagcgcc tcgaccagct ccgggaagta
     1501 gcggcccagg ggctggcagc tcttggactg gagcaacact tgttccccct tgcggaaggc
     1561 gaggcagcgg aagccgtccc acttcggctc gtagagccaa ccgccgtcac ctggagcggc
     1621 actcgggatg gcatcgacgg acttcgcctc catcgggggg aacggggggt ggacgggcag
     1681 gtcgatggcg gggaagtccc cctccagggc ctcggctggg agggccccgg ccacgacctc
     1741 gtcggcccgt ttctccggcg gcgacttctt gacgctgctc ttcctggctc cggcggttct
     1801 cataccggaa gcggggtgca agtggcttgc cggggcaacc ccccgggccc ggacggccag
     1861 gggccgttag ttgacggcgt gggggacggc caggacgaac ggcgcgatga ccgccttgaa
     1921 gccctcgcct cccaccggca ccatgaagaa ggagccctcc atggagccga ccgaggtggc
     1981 gagcggacag aagctggtgt actcgtagga ctcccccggg gcgagcaccg gctgctcgcc
     2041 gacgacgccc ggcccctgca ccctctgcac ctcgccgttg ccgtcggtga tgatccactc
     2101 gcggctcatg agctgcgcgg tcaccgatcc ttcgttggtg atccgtacgt gataggcaaa
     2161 gaagtagtag ctttcgtgcg gggcgctgcg gtcctcgtca tagaagctgt ggacctggat
     2221 gcggatcccc cgcgtggtcg tatcgctcat agcggcggca ttatgccacg cgttatgccg
     2281 tacgcctggc gcggcgactc tccggccgct gtggcataga atccgcacat ccgcggccag
     2341 acccggctga cgggaagcac cgcaggcaag aaaaaggggg agctgcgatg gaatggatta
     2401 gcaatccgga gagctggatc gcgttggcga cgctgacggc gctcgagatc gtgctgggca
     2461 tcgacaacgt catcttcatc tcgatcctgg cgggcaagct gccgcaggaa cagcaggcga
     2521 aggcgcgcag ggtcggcctg atgctcgcca tgggcatccg cctcctgctt ctcgcctccc
     2581 tggcctggat ggcgcggctg accagcccgc tgttcaccgt ctggaaccag gagatctcgg
     2641 ggcgtgacct gatcctgctg ctcggcggcc tgttcctgct cggcaaggcg acgcacgaga
     2701 tccacgacaa gctggaaggc gaggaggggc acgccagcgc caaggtcgtg gcgtcgttca
     2761 ccgcggtgat cgtgcagatc ctgctgctgg acatcgtctt ctcgctcgac tcggtcatca
     2821 ccgccatcgg catggcgaac gagctcgccg tcatgatgat cgcggtggtc atcgcggtgt
     2881 tggtgatgct gctgtcggca gggaagatca gcgacttcgt cggccgtcat cccacggtca
     2941 agatgctcgc cctctcgttc ctgctgctga tcggcatgtc gctgatcgcg gaagggttcg
     3001 accagcacat ccccaagggc tacatctact tcgccatggg cttctcggtc ttcgtcgaga
     3061 tgctcaacct gcgggccaag aaggtcacgg gggaggcgcc ggtgcagctg caccagcgct
     3121 acgccccgga ggcgccgaag gcccccaagg cgccggcgaa ggggcgggga aggaagtagg
     3181 aagagtggag agggggggcg cgccgcgccc cccttcggca aaccctgccg cgcctcaccc
     3241 cagccggaac tcgcgggtct cgccgtcgga gaactcgagg acgaggtgat ccccctggac
     3301 ggcgcggacc tcggcccggc catacatcgc cagcgtcagg acgtccccct gctgcaggtc
     3361 gcccggcttg ccccctccca ggagcggcgg caggggcagc acctccaccc gggatggcac
     3421 ccggaccggc ggctcggcgc gcggcaccac gggctcggtg aacggccggt cacagccgtc
     3481 gcagttggcg cagcgctccc actccgccgt ctcgccgaag tagtcgacga tcgccttcca
     3541 ccggcagagc gcggtctgac agtagagcac catgcgccgg agccggtcgc ggtccccctc
     3601 ggcacgctgc tcgtagcgct tgcccatcgc cgccagctcc tcgccggaga ggccgccgcg
     3661 cagcagcttg aagtggccgg gctcggtctc ctccaccagc tctaggtcct tcatcgtggc
     3721 gaggacgacc cgcaccttcg ccttggccac cccgcccgcc cgctcctgga cgtcccccgc
     3781 cgagacctcg cgccggtcgg ccttgaggcg ctccagggcc tggtagacgg cgtcgaagtg
     3841 ctcgcgcttc gggtagcggc cgttcaggaa gaagatctgg gtgttgcggt cctcggccag
     3901 gtagagcagc acgcagcggg cccgctcgcc gtcgcgcccc gcccgcccgg cctcctggta
     3961 gtacgactcc agcgagccgg ggaggttgta gtggatgacg aagcggatgt ccggcttgtc
     4021 gatccccatg ccgaaggcgt tggtggcgat gatggccttg agctggccgg acatgaagcg
     4081 gtcctgggtg gaccggcgct cgcgcgtccc cagccgcccg tggtagcgcg ccacgtcaaa
     4141 gccgaggctg gtgaggtggt cggtcacctc ctcgcagtcg cgcaccgtgg agcaatagac
     4201 gatgcccgtc ccctccatct cgccgagcag gcgggcgagg tgcgtgcgct tgatctcgtc
     4261 gttgcccacc gggatcacct cgaggtgcag gttgtcccgg tagaccccgg tggcgatgac
     4321 ctccatctcc tcgcggccga gcatgcgccg gatgtcgtcg atcacctgct ccgtggcggt
     4381 ggcggtcagg gcgaggaccg gcgggttgcc gagcgcctcg atcacctccc gcagatgcag
     4441 gtaggagggc cggaagtcgt gaccccactg tgagatgcag tgtgcctcgt cgatcacgaa
     4501 gacgtcgatg cggttcttcc gcagggtctc caggaactcc ggctgggtca cccgctcggg
     4561 cgtcacgaag acgaagtccc gctgctcctg gcggatctcc tccaggacct ccccctcctc
     4621 ccgcgccgtc agcgtactgt tgacctcggc cgcgtcgatc cccagctcct ggagcttgtc
     4681 gacctggtcc ttcatcaggg agaggagcgg cgacaccacc accgtggcgc cggggagctc
     4741 cttgcccggg agctgatagc agagggactt ccccgccccc gtcggcatga tcgccagggt
     4801 gtcgcgtccc tccatcacgg agtggatcac ctcttcctgg ccttcacgca gggatctgaa
     4861 gccgaacgta cgccgcaggg tacgccggag attgggtaga gtggtctgaa tgctttccgc
     4921 catggcaacc ttccgcgatt ccccttcagc gatccaaaag tggagcccgt gggcctgagc
     4981 cgacaggttg aagcaacgaa cgtgcctcct ccttcgccga tgaaaaagga ccgcgggctg
     5041 aacgccgagg ccctctcatc cgtataagag attgtgcggc gggcgttgac gaatcgcgcc
     5101 gcggcgatta gactctttcg aacaaatagt caaaggggta ttggcagttc ccgcagtgcc
     5161 gccccggagg cccgcccgga tgggtcaggc ctccctcggc ggaagcgccg gcatcggtga
     5221 caaccggggg gacggcgggg ctatcggttc ccctgaacga gacggaatgg acgaggttct
     5281 tatggtgact ttctttgcaa ctctgatggt cggggcagtg gctggcttca tgctcgagaa
     5341 gcagtacgga caggggatga cccgctggta ccgcatgcag aagatgcgcg cgcgggtcga
     5401 gcggcggctg ggcgagagcc aggattaggc cgttcggcgc tgaccgccgg cgcgccgggg
     5461 gcgcctggcg cggtgcgggt catccggtgc aggtgatccg cacccggccc caggcgttgt
     5521 tcgcgtaccc cttgaagttc cacacctgcg cggggtgctc gggttgggtc tgggcggccg
     5581 aatcccaggc gcggcagacg atctcgtgct cgcccgccgg cagctcgagc cgcgcctccc
     5641 agagccgcca ggcccagcgg ccctgctccc cccccagccc ggcggtgcgc cacgtggcac
     5701 ccccgtcgag agagaggtcc acccgcgcga ccgaccggcc gccgcccgcc atcgcgtagc
     5761 cctgcaccac cacctccccc gccggcaccg tctcaccctc cgccggagca cagatcaccg
     5821 agttgacggg gagctccccc agcatcaggc cgctctccca gtccacgtcc tgcgccgaca
     5881 cctgcggccc gaacagccgg taggccttcg cctggaagta gttgccggac ggctcgtcct
     5941 ggacggtgat ggccgacagc cacttgacgc tgcgggcgcc gatgtagccc ggcaccacgg
     6001 cccgcaacgg cgcgccatgg accggcggca gcggcgcgcc gttcatctcg taggcgagca
     6061 gcacctcgga cgacatcgcc ttctccagcg ggatcgagcc gccgaagcag aagcggtggc
     6121 cgtgccgctc ggtctcgtcg aggccggtga aggcgacatg cccggccccc ggctccggcg
     6181 cgaccgcggc cagcaggtcg cgcagggcga cgccccccca cttcgcggtg ctgatcgcct
     6241 ccgcccccca ccccagctcc cccgggatcg gcgcgaccgc cgccatctcg agccggcggt
     6301 tgccggcgca ctggagcgtg gcgtccaccg tcatcctggg gagccggcgc agctcttcga
     6361 gcgagaagcg cagcggccgc tgcacccggc cgccgaccac cagccggtag gacgaggcgt
     6421 cgacctccgg caccgcgccg tggttgcgga cgaagaacag gtcgttgggg gtgaggaagg
     6481 agcgccgcag gagcggcagg tccggaccgc cgttgaaggg gtcctcctgc cgcaccagga
     6541 gcccccggtg cttgccgaac ggggtggtca tgagggcctc agtcgtcgta ggtcaggggg
     6601 cggaggcgct tctggtagag gccgccggcc aggggctcga agatggtgca gtggtccatc
     6661 tcgccgccgt agacgtgcag ggtgagagag gtgcggtcgg gctggaggtt ggcgaggacg
     6721 tggtgctcga agggggggat cagcgcgccc gcgctcccgg ccacggccag ggcccggccg
     6781 cgcgcggcga agcggcagac gccgtcctcc tgctcctcca gcagctcgta gcggctgacc
     6841 tccatctccc cctggaccac cccctcgacg caccagatgc ccgcgtggtc gtggagcgc
//
LOCUS       DASPJI010000053         4722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_61942, whole genome shotgun sequence.
ACCESSION   DASPJI010000053 DASPJI010000000
VERSION     DASPJI010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61942"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4722)
                     /locus_tag="VEL74_01385"
     CDS             complement(<1..>4722)
                     /locus_tag="VEL74_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61206.1"
                     /translation="ASLEAWAHPDVPFETLVADLAPGRDLRSTPLVQTLFTLQAEPLR
                     LRLPGLSAAVEELDTGTAKFDLTLSLVEEMGGLAGTLEYDAGLFEAATIARLAGHLRV
                     LLAAAVAGPESRIGDLPLLTREERHQLAEWSPPPPPGLPAVCPHELFERWAAEKPDAV
                     AVVSGGESLTYGELDRRAGGIARRLRRVGVGPEVLVGLCVEPGLDLVTGMIAVFKAGG
                     AYVPLDPGYPRQRLAQIAEGTGLGVVLAQRHAAAALPEGGFRTIFLEESGTEEGARPA
                     SGVTSANLAYLIHTSGSTGRPKGVAIAHGSVVHLLGIARWRGLGEGDVWALVHSSSFD
                     ISVWETWGCLGLGGRLVVVPRAVIQAPGELARVLREEEVTVFTQTPAALRHLAEAGEL
                     AAGRTPALRLLISGGEAFPQELTAELLAWGPEVWDLYGPTETTVWAAGGRVEGGDGTA
                     SLGGALPGYELHVLDRNGHLLPLGVAGELHAGGPGLTRGYFRDPAATAEKLVPHVGGR
                     PGERLYRTGDLARRLPDGRLELLGRIDHQVKVRGFRIEPGEIEVALLAHPGVARVAVV
                     APVEPGGGRRLVAFFVPRAGAKVTGAELRTALRESLPEHMVPAALVELPELPLSPSGK
                     VDRKALLERVPVDGSDGEVVSAPPRTPVEELVAGLWSSVLGIERIGRSDNFFALGGHS
                     LLATRVLSRLHGAFGVDLPLRAVFEEPTVAGLATRVQAVLGGGRGGAPLAPPLVPMPR
                     PARPAGGLPLSFAQQRLWFLQQLAPESAFYNMPAALRLTGPLDEPALERSFDEIVGRH
                     EALRTRFPLRGDRPEQVIDPPERRPLPVVDLLGLPAAVKEDEAARLSLLEARHAFDLA
                     CGPLLRTVLLRLEPESHLLLVDMHHVVSDGWSIAVLYQDFAALYGAFAAGRPSPLPPL
                     PVQYADFTLWQREWLQGEVLQAELDHWRRRLADPPPPDLPADRPRPAAQSFRGGTLRF
                     AIPRQLTEELRRLARDRGVTLFMALLAGFKALVQRYTGREDVALGSPIANRDRAEIER
                     LVGFFVNTLVLRTDLSGEPGFRELAARVREVCLDAYAHQALPFERLVEELEPERDLAR
                     QPLFGTMFQLLEMPPESVAVGPLTLERLEVETGTAAFDLALDLVDGPEGILVKAVHSA
                     DLFDTVTIRRLLEHYISLLAAVAGGDERPVVEIEILSPAERHQLLAEWNEQPAAPSEG
                     KLLHEGFAAQVERAPDVVAVALDEQSLTYGELARRARRLAHRLRAVGIGPEVPVAVSL
                     ERSPEMVTSLLGVLESGGVYLPLDPALPRERQAFLLEDSGADIVIATAGTARRLPPSG
                     ARHILIDKEEDGADGAAPPLPAIDPASLAYVIYTSGSTGRPKGVGVSHAAASAHCRAV
                     ARFYGLGPEDCVLQFASASFDVSFEEILPTLGSGARIRLRPAQGWSPAELLQRLADFG
                     LTVVGLPTAFWHQLAEEAERGTPPQAGHRIRLMVAGGEAMPAEALRRWHAGPLAGIPL
                     LNAYGPTEAVVTSTAADLSSPEAPATGRVPIGRPLAGRTVQVMDRRGAPVPMGVAGEL
                     WIGGPLLARGYLGRPELTAER"
BASE COUNT          697 a         1802 c         1694 g          529 t
ORIGIN      
        1 aaccgctccg ccgtcagctc cgggcggccg aggtaccccc gggcgaggag cggcccgccg
       61 atccacagct ccccggcgac gcccatgggt accggcgctc cccggcggtc catcacctgg
      121 accgtccggc cggccagcgg gcgcccgatg gggaccctgc cggtcgcggg ggcctcgggc
      181 gaggagagat ccgccgccgt cgaggtgacc acggcctcgg tgggaccata ggcgttgagc
      241 agggggatac cggccagcgg tccggcgtgc cagcggcgga gcgcctcggc cggcatggcc
      301 tctccgccgg cgaccatcag ccggatacgg tgccccgcct gaggcggcgt accccgctcc
      361 gcctcctccg cgagctggtg ccagaaggcg gtggggagcc ccaccaccgt gaggccgaag
      421 tcggcgagac gctggagcag ctccgcaggg ctccacccct gtgcggggcg caggcgaatc
      481 cgcgcaccgc tccccaaggt cggcaggatc tcctcgaagg acacgtcgaa gctcgccgac
      541 gcgaactgca ggacgcagtc ctccgggccc aatccataga agcgggccac cgcccggcag
      601 tgggccgagg ccgctgcatg ggagacgccg acccccttgg gccgccctgt cgagcccgag
      661 gtgtagatga cgtaggccaa gctcgcggga tcgatcgcgg ggagcggcgg ggcggcgccg
      721 tccgcgccgt cctcttcctt atctataagg atgtgccggg cgccggaggg gggcaggcgc
      781 cgggcggtgc ccgccgtggc gatcacgatg tcggcgcccg agtcctccag gaggaaggcc
      841 tggcgctccc ggggcagggc cggatccagg ggcaggtaga cgccacccga ctccagcaca
      901 cccagcagcg acgtcaccat ctccggcgag cgctcgagag agaccgccac cggcacctcg
      961 ggcccgatgc ccaccgcccg caggcggtgg gcgaggcggc gggcgcggcg ggcgagctcg
     1021 ccataggtca ggctctgctc gtccagcgcg acagcgacca catccggagc ccgctccacc
     1081 tgggccgcga agccctcgtg gagcagcttg ccctcggacg gagccgccgg ctgctcgttc
     1141 cactccgcca ggagctggtg ccgctcggcc ggcgagagga tctcgatctc cacaacggga
     1201 cgctcgtcgc cgccggccac ggccgcgagc agggagatgt agtgctccag gaggcggcgg
     1261 atggtgaccg tatcgaacag gtccgcgctg tggaccgcct tgaccaggat cccctccggg
     1321 ccatccacca ggtcgagggc gaggtcgaac gccgcggtcc cggtctccac ctccaggcgc
     1381 tccagggtga gcgggccgac cgcgacgctc tcgggaggca tctccagaag ctggaacatg
     1441 gtgccgaaga ggggctggcg ggcgaggtcc cgctccggct ccagctcctc caccaggcgc
     1501 tcgaacggca acgcctggtg cgcgtaggca tccaagcaga cctcccgcac ccgcgccgcc
     1561 agctcccgga agcccggctc ccccgacaga tcggtccgca gcaccaaggt gttgacgaag
     1621 aagcccacca gccgctcgat ctcggcgcgg tcgcggttgg cgatcggcga gcccagggcg
     1681 acgtcctccc ggccggtgta gcgctggacc agggccttga agccggcgag gagagccatg
     1741 aaaagggtaa cgccacggtc ccgggcgagg cgccgcagct cctccgtcag ctgccgcggg
     1801 atggcaaagc ggagcgtccc gccgcggaag ctctgggcgg cgggtcgcgg cctgtccgcc
     1861 ggcaggtccg gcggcggcgg atcggcgagg cgccggcgcc agtgatccag ctccgcttgc
     1921 agcacctctc cctggagcca ctcgcgctgc cagagggtga agtcggcgta ctggaccggc
     1981 agcggcggca ggggggaggg cctgcctgcc gcgaacgctc catagagagc ggcgaagtcc
     2041 tggtagagca cggcgatgga ccagccgtcc gacaccacgt ggtgcatgtc gaccagcagc
     2101 aggtgactct ccggctccag acgcagcagc accgtccgca ggagcgggcc gcatgccagg
     2161 tcgaaggcgt gccgggcttc cagaagcgag agccgggccg cctcgtcctc cttcaccgcc
     2221 gcgggcaggc ccagcaggtc cacgaccggc agcggccgcc tctccggagg gtcgatcacc
     2281 tgctccggcc ggtccccacg cagcgggaag cgggtacgga gcgcctcgtg ccggccgacg
     2341 atctcgtcga agctccgctc cagcgccggc tcgtccagag gccccgtgag ccggagcgcc
     2401 gccggcatgt tgtagaaggc gctttccggc gcgagctgct gcaggaacca gagccgctgc
     2461 tgggcgaagg agagcggcag cccccccgcc ggccgcgccg gccgcggcat gggcaccagc
     2521 ggcggcgcga gcggagcacc gccccggccg ccacccagca ccgcctgtac ccgcgtggcg
     2581 agccctgcca ccgtgggctc ctcgaacacg gcgcgcagcg gcagatccac gccgaacgcc
     2641 ccgtgcaagc gagagagcac gcgggtggcg agaagcgagt ggccgccgag agcgaagaaa
     2701 ttgtcgctgc ggccgatccg ctcgatcccc agcaccgagg accagagacc cgccaccagc
     2761 tcctccaccg gcgtcctcgg cggggcggag accacctcgc cgtcgctgcc atccaccggc
     2821 accctctcca acagggcctt ccggtccacc ttgccgctgg gcgacagcgg cagctcgggc
     2881 agctccacca aggcggcggg caccatgtgc tccgggaggc tctcccgcag ggcggtccgc
     2941 agctctgccc cggtcacctt agcgccggcg cgcgggacga agaaggccac cagccgccgg
     3001 ccgcctcccg gctccaccgg cgccaccacc gccacccgcg ccactcccgg atgggcgagg
     3061 agcgccacct cgatctcccc gggctcgatg cggaacccgc gcaccttcac ctgatgatcg
     3121 atgcgcccga gcagctccag ccggccgtcc ggcaggcgcc gcgccaggtc gccggtgcgg
     3181 tacaggcgct cccccggacg gccacccacg tgcggcacga gcttctccgc cgtggccgcc
     3241 gggtcccgga agtagccgcg cgtgaggccg gggccgccgg cgtgcaactc tccggcgacg
     3301 cccaggggaa gcaggtggcc gttgcggtcg agcacgtgca gctcgtagcc gggcagggct
     3361 ccgccgagag aggcggtgcc gtccccgcct tcgacccggc cgcccgccgc ccagaccgtg
     3421 gtctcggtgg gtccgtagag gtcccatacc tccggtcccc aggcgagcag ctccgccgtc
     3481 agctcctgcg ggaaggcctc gccgccgctg atcagcaggc gcagcgccgg ggtccggccc
     3541 gccgccaact ccccggcctc ggcgaggtgg cgcagggccg cgggggtttg cgtgaagacc
     3601 gtcacctcct cctcccgcag cacccgggcg agctcgccgg gagcctggat cacggcgcgc
     3661 ggcacgacga ccagccggcc gcccaggccc aggcagcccc aggtctccca gaccgaaatg
     3721 tcgaacgagg aggagtggac cagggcccag acgtccccct cgcccaggcc ccgccagcgg
     3781 gcgatcccca acaggtggac gacgctgccg tgggcgatcg ccaccccctt gggccggccg
     3841 gtggagcccg aggtgtgaat cagataggcc aggttggcgg aggtcacccc ggaggcaggc
     3901 cgggcgccct cctccgtacc gctctcctcc agaaagatcg tccggaagcc cccctcgggg
     3961 agagccgcgg cggcgtgccg ctgggccagc accaccccga gccccgtccc ttcggcgatc
     4021 tgcgccaggc gctgccgggg gtagcccgga tcgaggggga cgtaggcgcc gccggccttg
     4081 aagacggcga tcatcccggt caccaggtcg agtcccggct ccacgcacag gccgacgagc
     4141 acctcgggcc cgacccccac acggcgcagg cggcgcgcga tccccccggc ccggcggtcg
     4201 agctcgccgt aggtcaggct ctcgccaccg gagacgacgg ccaccgcgtc gggcttctcc
     4261 gccgcccagc gctcgaacag ctcgtgcggg cagaccgccg gcaggcccgg tggtggagga
     4321 gggctccact cggcgagctg gtgccgctcc tcccgggtga gcaggggcag atcgccgatc
     4381 cggctctccg gccccgccac cgccgccgcc agcagcaccc gcagatggcc ggcgaggcgg
     4441 gcgatggtgg ccgcctcgaa gagaccggcg tcgtactcca gagtccccgc gagcccgccc
     4501 atctcttcca ccagagacag tgtcaggtcg aacttcgccg tcccggtgtc cagctcctcc
     4561 accgccgccg agaggccggg cagccgcagc cggagcggct ccgcctggag ggtgaagagg
     4621 gtctggacga gcggggtgct ccgcaggtcc cggcctggcg cgagatcggc caccagcgtc
     4681 tcgaagggga cgtccggatg cgcccaggct tccagcgagg cg
//
LOCUS       DASPJI010000054         4030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_463487, whole genome shotgun sequence.
ACCESSION   DASPJI010000054 DASPJI010000000
VERSION     DASPJI010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463487"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /gene="ybgF"
                     /locus_tag="VEL74_01390"
     CDS             <1..695
                     /gene="ybgF"
                     /locus_tag="VEL74_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HYG61207.1"
                     /translation="EEVSSLEASVGRQMGQLLKTEADMQVKLQEVSTQIDELQARLED
                     TNDRLAQLSQQIAATNQELRTFRNTQPAPVTDPGMGGGTSPIGGQPPQTLPGGAGGGS
                     ADPKALYDAAYNDYLKGNYDLSVRQFEEYLANFPGTELADNATYWIGESYYRQKRFRQ
                     AIDQFDEVLRQYPRSDKAAGAVLKKGYAHIELGERTQGVTQLRGVIRQHPNSDEAKLA
                     RQRLRELGVDAG"
     gene            804..1079
                     /gene="rpsT"
                     /locus_tag="VEL74_01395"
     CDS             804..1079
                     /gene="rpsT"
                     /locus_tag="VEL74_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HYG61208.1"
                     /translation="MANTKSAEKRNRQNLKVRERNRGQRSRLRTAIKRLRTAVTGGDA
                     AQAEAMLRETFGVIDKTAQKGVIHKNAASRYKSRLAQRVAALGQKAS"
     gene            1187..1627
                     /locus_tag="VEL74_01400"
     CDS             1187..1627
                     /locus_tag="VEL74_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61209.1"
                     /translation="MERLSLKALSALLLLILLLPVVACDSDDGNEGPTAPGPPLFIAG
                     LWNGTFTPDGGGTAAVALELEQSGRLVTGLVAVGGLTWAIEGSVDSLGRFEWDTEGAT
                     CGSFDATMLVDRSRTTMAGSAQLDRFACLEGRRQRGDLVLQKVR"
     gene            complement(1624..2145)
                     /locus_tag="VEL74_01405"
     CDS             complement(1624..2145)
                     /locus_tag="VEL74_01405"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HYG61210.1"
                     /translation="MKPCAAVSSILLLAACLSGCGYALVGRASNIPEDIREVYIHPFE
                     NRTQRSQVEQEVTRSIAEEMVNRQRFSVVGGVEESDAELIGAVTAFGVTPVTFDETGR
                     ATEYEISITAQVSFKRKGSDELIWSNDRYVFRESYPIDPDSPDYFDRENEAIREAAER
                     FAESMVADLLEGF"
     gene            complement(2162..2662)
                     /gene="nusB"
                     /locus_tag="VEL74_01410"
     CDS             complement(2162..2662)
                     /gene="nusB"
                     /locus_tag="VEL74_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HYG61211.1"
                     /translation="MAREMAVQMLYQSDLGGSPLPQIFNTFDLTEYLTRDENGNEAQP
                     GDKKPDLKGDRAEYAKRRKRVEEAFHFAQTLVDGAMQHREEVDALIRSQADNWRLERM
                     PAVDRNILRLAIYEMLYEKETPKLVVVDEAIELAKKFGSEQSGRFVNGLLDGLLKQHT
                     FPGSLT"
     gene            complement(2839..3357)
                     /gene="ribH"
                     /locus_tag="VEL74_01415"
     CDS             complement(2839..3357)
                     /gene="ribH"
                     /locus_tag="VEL74_01415"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HYG61212.1"
                     /translation="MNDIRETQGSLQGTGRRFGIVASRFNSRIVDLLVAGAVDCFRRH
                     GVEPGDIHVVRVPGAWEIPAALEELAALGNVDGLVALGVVIRGETPHFDYICGECSGG
                     VARVADRHRLPVGFGVLTCDTSEQAQERAGGKAGNKGWEAALAVLEMADLFARLRASA
                     PASASAFASKSG"
     gene            complement(3370..>4030)
                     /locus_tag="VEL74_01420"
     CDS             complement(3370..>4030)
                     /locus_tag="VEL74_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61213.1"
                     /translation="GALFYLPLIAPLTLYPSFARRVRERDYFGAWSRGMAWALLLSLG
                     VILLVFWLPDAARHGIFNGEPYRVEMFGWIGTGAGPENDWRAFLPTHLLHLAVFLILT
                     WLSAGYLGLTLGAALVGYMSYFVGTYAMASGHPVLGSMAAWVPWSVVRVLAFVLFGCL
                     FARPLLVRRLWPFEREEYRLMGLATAGIVIDILMKAFFAPSYGRFLRQMAGGALAFLF
                     P"
BASE COUNT          691 a         1373 c         1392 g          574 t
ORIGIN      
        1 aggaagaggt gtcgagcctg gaggcctcgg tcggccggca gatgggccag ttgctcaaga
       61 ccgaggccga catgcaggtc aagctgcagg aggtgtcgac gcagatcgac gagctgcagg
      121 cccggctcga ggacaccaac gaccggctcg cgcagctctc gcagcagatc gccgccacca
      181 accaagagct gcggacgttc cgcaacaccc agccggcccc ggtcaccgac cccggcatgg
      241 gcggcggcac ctctcccatc ggcggccagc cgccgcagac cctcccggga ggggcgggcg
      301 gcgggagcgc cgaccccaag gccctgtacg acgcggccta caacgactac ctgaagggca
      361 actacgacct gtccgtccgc cagttcgagg agtacctggc caacttcccc ggcaccgagc
      421 tggcggacaa cgccacctac tggatcggcg agagctacta ccggcagaag cgcttccggc
      481 aggcgatcga ccagttcgac gaggtgctgc ggcagtaccc gcggagcgac aaggccgcgg
      541 gcgcggtgct caagaagggc tatgcccaca tcgagctggg cgagcgcacc cagggggtga
      601 cgcagctccg cggcgtcatc cgccagcacc ccaacagcga cgaggcgaag ctggcgcggc
      661 agcggctgcg cgagctcggg gttgacgcgg gataagcagg ggtgctataa atccctgtcg
      721 cgtcgcactc cggtgcgacg ttcctgtgtc agggcatccc ccacacatac gatcggaaga
      781 ctgagaccat accgggacga ataatggcca atacgaagtc tgcagagaag cggaaccgtc
      841 agaacctcaa ggtgcgggag cgcaaccgcg gtcagcgctc gcgcctgcgc accgccatca
      901 agcgcctccg caccgcggtg acgggcggtg acgcggccca ggcggaggcg atgctgcgcg
      961 agaccttcgg cgtcatcgac aagacggcgc agaagggcgt gatccacaag aacgccgcgt
     1021 cgcgctacaa gtctcgcctt gcccagcggg ttgccgctct cggccagaag gcgagctaag
     1081 ctcccctcgc atcggctctc acgcctcggg cggcccctcc gggccgccct ttcggcgctt
     1141 cagccggctc ttcagcggcg gcacggccca tgcaaaagcc tcgggcatgg aacgcttgtc
     1201 gttgaaagcg ctatccgccc tcctgctcct catcctgctc ctgccggtcg tcgcctgtga
     1261 ctccgacgac ggcaacgaag gccccacggc gccgggtccc ccgctcttca tcgccggcct
     1321 ctggaacggc accttcacgc ccgacggggg cggcaccgcc gcggtcgccc tggagctcga
     1381 gcagtcgggc cgcctggtca cggggctggt ggccgtaggc ggcctcacct gggctatcga
     1441 agggagcgtc gactcgctcg gccgcttcga atgggatacg gagggggcga cctgcggctc
     1501 gttcgacgcc acgatgctgg tggaccggtc gcggaccacc atggccggca gcgcgcagct
     1561 cgaccgcttc gcctgcctcg aagggcggcg ccagcgcggc gacctggtgc tgcagaaggt
     1621 gcgctaaaag ccttcgagga ggtccgccac catggactcg gcgaagcgct cggcggcctc
     1681 gcggatggcc tcgttctcgc ggtcgaagta gtccgggctg tcggggtcga tgggatagct
     1741 ctcgcggaac acgtagcggt cgttgctcca gatcagctcg tcgctcccct tgcgtttgaa
     1801 cgacacctgg gcggtgatgg agatctcgta ctcggtggcg cggccggtct cgtcgaaggt
     1861 gaccggggtg acgccgaagg cggtgaccgc accgatcagc tcggcgtcgg actcctcgac
     1921 tccgcctacc accgagaacc gctggcggtt caccatctcc tcggcgatcg accgcgtcac
     1981 ctcctgctcc acctgggagc gctgggtgcg gttctcgaac gggtggatgt agacctcccg
     2041 gatatcctcg gggatgttgc tcgcccgacc taccagggcg tagccgcagc cggacaggca
     2101 ggcggccagc aggaggatgg aagagacggc ggcgcagggc ttcatgagga cctccggagg
     2161 tctaggtcag gcttccaggg aatgtgtgct gcttgagcag gccgtccagc aggccgttga
     2221 cgaaccggcc cgactgctcg gacccgaact tcttcgccag ctcgatggcc tcgtcgacca
     2281 ccacgagctt gggcgtttcc ttctcgtaca gcatctcgta gatcgccagg cgcaggatgt
     2341 tgcggtcgac ggcgggcatc cgctccagcc gccagttgtc ggcctggctg cggatcaggg
     2401 cgtcgacctc ttcgcggtgc tgcatggcgc cgtcgacgag ggtctgggcg aagtggaacg
     2461 cctcctccac ccgcttccgg cgtttggcgt actcggcgcg gtcgcccttg aggtcgggct
     2521 tcttgtcccc tggctgcgct tcgttgccgt tctcgtcccg ggtgaggtac tcggtcaggt
     2581 cgaaggtgtt gaagatctgc ggaagggggc tgcctcccag atcgctctgg tagagcatct
     2641 ggaccgccat ctcccgtgcc atgcgccgct ttcctgccgt catgcactcg cctcaaagat
     2701 ccgggcccgc cacagcgggc agtccctcca aaatgacatg gaaaactaca gactccatcg
     2761 acaacaaggc cgctcccaga ttgccgggaa cggccgcgtc acgcgccggc acatgatagc
     2821 cgggcggcgc cggttctgct agccgctttt cgaggcaaaa gccgaggctg aagccggggc
     2881 cgaggcacgc agacgggcga agaggtcggc catctcgagc acggcgagcg ccgcctccca
     2941 ccccttgttg ccggccttgc cgccggcgcg ctcctgcgcc tgctcggagg tgtcgcaggt
     3001 gagcacgccg aagcccaccg gcagccggtg gcggtcggcc acgcgggcca cgccgccgct
     3061 gcactcgccg cagatgtagt cgaagtgggg cgtctcgccc cggatcacca cgcccagcgc
     3121 caccaggcca tcgacgttgc cgagcgccgc cagctcctcc agcgccgccg ggatctccca
     3181 ggcgccgggt acccgcacga cgtggatgtc tccgggctcg acgccgtggc gtcggaagca
     3241 gtccacggcc ccggcaacca gcaggtcgac gatccgggag ttgaaccggg aggcaacgat
     3301 gccgaagcgc ctgcccgtgc cctggagaga gccctgcgtt tcacggatgt cgttcatggg
     3361 atcggtccct caagggaaaa gaaaggcgag agcgccgccc gccatctggc gcaggaagcg
     3421 gccataggag ggggcgaaga acgccttcat caggatgtcg atgacgatgc cggctgtggc
     3481 gagccccatg agccggtatt cctcgcgctc gaagggccag aggcggcgga cgagcagcgg
     3541 ccgggcgaag aggcagccga agaggacgaa ggcgaggacg cggaccaccg accaggggac
     3601 ccaggcggcc atcgagccga ggacggggtg accgctcgcc atcgcgtacg tgccgacgaa
     3661 gtagctcatg taccccacca gggcggcccc cagggtgagg ccgaggtagc cggcgctcag
     3721 ccaggtgagg atcaggaaga ccgccaggtg cagcaggtgg gtgggcagga acgcccgcca
     3781 gtcgttctcc ggcccggcgc cggtgccgat ccagccgaac atctccaccc ggtagggctc
     3841 gccgttgaag atgccgtggc gggcggcgtc gggcagccag aagaccagga ggatgactcc
     3901 caggctcagc agcaaggccc aggccatgcc ccggctccag gcgccgaagt aatcccgctc
     3961 gcgcacccgc cgggcgaagg agggatagag ggtgagcggc gcgatcagcg gcagatagaa
     4021 gagggcgccg
//
LOCUS       DASPJI010000055         2929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_575467, whole genome shotgun sequence.
ACCESSION   DASPJI010000055 DASPJI010000000
VERSION     DASPJI010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575467"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1761)
                     /locus_tag="VEL74_01425"
     CDS             complement(<1..1761)
                     /locus_tag="VEL74_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG61214.1"
                     /translation="MNTLPSDLRQAARSLFRRPGFLAVAVLTLALGIGANSALFSVVH
                     AVLMRPLPYQDAERLVWITHEVPVFGTETVVGADFLDWREGSRTLSGIAGYDDSASVT
                     LTGRERAERLHGARVSPNFLALLGARPAQGRDFLPEEERLNAGNTAILSHRLWERLFG
                     ARTTIDETPLLTLDGNGFTVVGVLPPDFRFPGNPEIDVLVPLALDSAVERGRTRMSLL
                     SAVGRLAPGASLEQARAELDAIHRRGEESFRPSPSSAPPPGGPSNVSPRGSTRQFSMA
                     PGSLGLPEMILKVVPLREHLVGDARPSLLMLLGAVGFVLLIACANVANLLLARATARQ
                     REIAVRAALGAGRFRIARHLLLESALLGLMGGACGLALAALGVRLLVTAVPAELAGGA
                     FRQIPIGIDGTVLLFTLGLSLATGLLFGLAPAMAASRPDLQAPLKQGGRAGGGSNAAR
                     RLLVAAEVALAAVLLIGAGLLFRSFLRVGTVDPGFRPEGVLTAALELDTGRYAAPASQ
                     AGFAQTLAERVRGLPGVEAAGFGDTVPLAGFNMLVGGPRAEGLPEQEDPLQVAMVVAT
                     PGYFEAIGIPMRRGRAFTEED"
     gene            complement(1767..2882)
                     /locus_tag="VEL74_01430"
     CDS             complement(1767..2882)
                     /locus_tag="VEL74_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HYG61215.1"
                     /translation="MPPWRPRYSLTPGIARGLMEIEAARVLVEHVSLPPTVQAELRRR
                     ARVRSTHYSTRIEGNRLTLAEAERVIEGRRVQLHGRERDVGEVRHYWNAHLRVEEWAE
                     RGAPLTKELVRRLHALVESGRARPTPYRTGQNSIRDSETGAIVYLPPEAKDVPELMAS
                     FIDWERQAREENLPVPIIASLAHYQFVSIHPYYDGNGRTARLLATFLLHRGGYGLKGL
                     FSLEERHARDLAAYYSALRVHPHHNYYEGPNKADLTQWLEYAVDTLAAVFVEAKEEAL
                     RHAAAGIPEIPEALRQLDARARVVLGLFSRQERVTAPDVASALGLSDRMARVLLKEWV
                     DQGWIRVADPSRRARSYDLSAIYRQFIGSTSAMPSED"
BASE COUNT          455 a         1016 c         1073 g          385 t
ORIGIN      
        1 gtcttcctcg gtgaaggcgc gcccccgccg catggggatg cctatcgctt caaaatagcc
       61 gggcgtggcc accaccatcg cgacctgcag cgggtcctcc tgctcgggca gcccctccgc
      121 ccgggggccg ccgaccagca tgttgaagcc ggcgagcggc acggtgtcgc cgaagccggc
      181 ggcctcgacc cccggcaggc cgcggacgcg ctcggccagc gtctgggcga agccggcctg
      241 cgaagcgggc gcggcgtagc ggccggtgtc gagctccagc gccgcggtga gcaccccctc
      301 cggccggaac cccgggtcca cggtcccgac gcgcaggaag ctacggaaga gcagccccgc
      361 cccgatcagc agcacggcgg ccagcgccac ctcggccgcc accagcagcc ggcgggcggc
      421 gttcgagcct ccgccggccc gtcccccctg cttgagggga gcctggaggt ccggccgcga
      481 cgccgccatc gccggggcga ggccgaagag gaggccggtg gcgagggaga ggccgagggt
      541 gaacagcagc acggtgccgt cgatcccgat ggggatctgc cggaaggcgc cgccggcgag
      601 ttcggccggg acggcggtca ccagcagccg gactcccagc gccgcgaggg cgagcccgca
      661 ggcgccgccc atcagcccga ggagcgcgct ctcgaggagg agatggcggg cgatccggaa
      721 gcgtccggcc ccgagagccg cccgcaccgc gatctcccgt tgccgtgccg tcgcccgggc
      781 caggaggagg ttggcgacgt tggcgcaggc gatcaggagg acgaacccca cggcgcccag
      841 gagcatcagg agggagggcc gggcgtcccc caccaggtgc tcgcgcaggg ggacgacctt
      901 caggatcatc tccggcaggc cgaggctgcc cggcgccatg ctgaactgcc gcgtggagcc
      961 gcggggcgag acgttcgacg gcccgccggg cggaggtgca gaggagggcg agggccggaa
     1021 cgactcctcc ccccgccggt gaatggcgtc gagctcggcc cgcgcctgct ccaggctggc
     1081 gccgggggcg agacggccga cggcgctgag cagcgacatc cgcgtccggc cgcgctccac
     1141 cgccgagtcc agggccaggg ggacgagcac gtcgatctcc gggttgccgg ggaaccggaa
     1201 atccgggggg aggacgccga cgacggtgaa gccgttgccg tccagggtga ggagaggcgt
     1261 ctcgtcgatg gtcgtccgtg cgccgaacag ccgttcccac agtcggtgac tgaggatggc
     1321 cgtgttgccg gcgttgagac gctcctcctc cgggaggaaa tcgcgcccct gcgccggccg
     1381 ggcgccgagg agcgccagga agttggggga gacgcgggcg ccgtggagcc gctccgcccg
     1441 ctcgcggccg gtcagggtga cgctggccga atcgtcgtac cctgcgatcc cggacagcgt
     1501 ccggctcccc tcgcgccagt ccaggaaatc ggccccgacc accgtctcgg tcccgaacac
     1561 cggcacctcg tgggtgatcc acacgagccg ctcggcatcc tgataaggaa gaggccgcat
     1621 cagcaccgca tgcacgacgc tgaacagcgc cgagttggcg ccgatcccca gcgccagcgt
     1681 gagaaccgcc accgccagga accccgggcg ccggaacagc gaccgcgccg cctggcggag
     1741 atcggagggg agggtgttca tgggagtcag tcctcgctcg gcattgccga tgtactgccg
     1801 ataaattgcc gataaattgc cgataagtcg taggacctcg cccggcgcga gggatcggcg
     1861 acccggatcc agccctggtc aacccattcc ttgaggagca cgcgggccat tcggtccgag
     1921 agaccgagcg cactggcgac atcgggggcg gtgacgcgtt cctggcgaga gaagagtccc
     1981 agcaccacgc gggctcgggc atcgagctgg cggagggcct cggggatctc cggaatcccc
     2041 gctgccgcgt gacgcagcgc ctcctccttg gcctcgacga acactgccgc cagggtgtcg
     2101 acggcatact ccagccactg agtgagatcg gccttattgg gcccctcgta gtagttgtgg
     2161 tggggatgga ccctgagcgc ggagtagtag gcggcgagat cgcgcgcgtg ccgctcctcc
     2221 agagagaaga gccccttgag cccatagcct ccccggtgga gaaggaaggt ggcgagcagc
     2281 ctggcggttc ggccgttgcc gtcgtagtaa gggtggatgc tgacgaactg atagtgggcg
     2341 agcgaggcga tgatggggac cggcaagttc tcttcgcggg cctgccgctc ccagtcgatg
     2401 aagctggcca tcagctccgg gacgtccttc gcctccggag gcagatagac gatcgccccg
     2461 gtctcggagt cacggatcga gttctgcccg gtcctgtagg gagtcggccg tgcccgcccg
     2521 ctctcgacga gggcatggag acgccggacg agctccttgg tcagaggagc gccgcgctcg
     2581 gcccactcct cgactctgag gtgggcattc cagtagtggc ggacctcgcc gacgtcccgc
     2641 tcccgtccgt ggagctggac tcgtcttccc tcgatgaccc gctcggcctc ggccagcgta
     2701 aggcggttcc cttcgatgcg cgtcgagtag tgggtcgagc ggacccgggc acgccgacgt
     2761 agctccgcct ggaccgtggg agggagcgag acgtgctcga cgagcacccg agcggcctct
     2821 atctccatga gcccgcgggc gatgcccgga gtcaggctgt agcgtggtcg ccagggaggc
     2881 atggccccct attgtagatg cggcccgccc cttccccctc aatccaccc
//
LOCUS       DASPJI010000056         3226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_518609, whole genome shotgun sequence.
ACCESSION   DASPJI010000056 DASPJI010000000
VERSION     DASPJI010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518609"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1626
                     /locus_tag="VEL74_01435"
     CDS             <1..1626
                     /locus_tag="VEL74_01435"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HYG61216.1"
                     /translation="SRRHAQIDVLPDGGVVIADAGSKNGTFVAGRRVRQAAVCGFGVV
                     SFGPVQALLQPADPTWDQVLLEPPGEPGGREPARRGSAAPLPSTRGLHPLERLAESLG
                     EVLPPLVEGLMRPEEAADELVHRWVAVLPIDRADILRAGAAGESIVAAASGSGRGQEA
                     VSFQVEGPEGWTLRLRAAADAELRPLRPLFRLVLDLLASGSGLRLRSGRRGDRTDRPD
                     RPDRPHEPPPGLGKEMTQLYRRAGKVARGDVAVLILGESGAGKEVLARWIHAGSRRAA
                     GPFLAINCAALPRELLEAELFGIERGVATGVEARPGILERATGGTVFLDEIGDMAAET
                     QAKVLRVLESTSLYRVGGRNPVAIDVRFLSATNRDLESMVEEGSFRRDLYHRLAAMEV
                     KLPPLRERREEIPALAARFFHRELERNGVASPGINRAALGALVQYPWPGNVRELQNEI
                     ARAALLLEPGEPLDIRHLSRRVRGAVAGGVPPVQALTLDETVARAEREAFRVALAAAG
                     GDAARAMELLGVSRTTYYRKLKELGLGESGGSG"
     gene            1739..2464
                     /locus_tag="VEL74_01440"
     CDS             1739..2464
                     /locus_tag="VEL74_01440"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HYG61217.1"
                     /translation="MTHTKRPALSLFAALLLSLCATGAAHAELVVLTDGNVMKVSAYE
                     VQAEQVSVTLGRGGRLTFPITRVERIVDDEIVPQPEPVPEVTAEAEAVASLIPLEFDE
                     SQPVPEGRYGTLIYETARKHSINPQIVAAMIRQESAGNPRALSHKGARGLMQLMPATA
                     HRFGVRKEKLYEPEHNLEAGVRYLTWLIDHFQGDLAKVLAAYNAGEGAVARHGGIPPY
                     KETRNYVKRIFGNLGLSVAGLPL"
     gene            complement(2722..>3226)
                     /locus_tag="VEL74_01445"
     CDS             complement(2722..>3226)
                     /locus_tag="VEL74_01445"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HYG61218.1"
                     /translation="MLKAALGVLVSETRDPEEILRRLDDVVRTNGERRFFVTATLALV
                     DLAAGRLQLINAGHPPTYLIRDGRTTEIMLPGSPLGALGKTYGRAAVDLRPGDYLVWL
                     SDGLVEAPNETGEPFGYESVVSALSGTHPARPEELRDRLVTAVARHAGGQPPSDDRTM
                     LVLGYRG"
BASE COUNT          412 a         1186 c         1206 g          422 t
ORIGIN      
        1 tcccgccgcc atgcccagat cgacgtcctg ccggacggcg gcgtggtgat cgccgacgcc
       61 ggctccaaga acggcacctt cgtggccggc cggcgggtgc ggcaggcggc ggtctgcggc
      121 ttcggcgtgg tctccttcgg cccggtccag gcgctgctgc agccggcgga ccccacctgg
      181 gaccaggtgc tcctcgagcc ccccggagag cctggaggcc gggagcctgc ccggcgcggc
      241 tccgcggcgc cgttgccgtc cacccgcggc ctccacccgc tcgagcgcct ggccgagtcc
      301 ctgggcgagg tgctgccgcc gctggtcgag gggctgatgc ggcccgagga ggcggcggac
      361 gagctggttc accgctgggt cgccgtgctg cccatcgacc gcgccgacat cctccgtgcc
      421 ggcgcggcgg gtgaatccat cgtcgccgcc gcctccggct cgggacgcgg gcaggaggcg
      481 gtctccttcc aggtggaggg gccggaggga tggacgctgc gcctgcgcgc cgccgccgac
      541 gccgagctcc ggccgctccg gccgctgttc cggctggtcc tggatctgct cgcgtccggc
      601 tccggcctgc gcctgcgcag cgggcgacgg ggagatcgga ccgatcggcc ggatcggccg
      661 gatcggcccc acgagccgcc gccgggtctc gggaaggaga tgacccagct ctaccgccgg
      721 gccggcaagg tggcccgggg ggacgtggcg gtcctgatcc tgggggagtc cggggccggc
      781 aaggaggtgc tggcgcgctg gatccacgcc gggtcgcgcc gcgccgccgg ccccttcctg
      841 gcgatcaact gcgccgccct gcccagggag ctgctggagg ccgagctgtt cggcatcgag
      901 cgcggcgtgg cgaccggcgt cgaggcccgg cccggcatcc tggagagggc gaccggcggc
      961 accgtcttcc tcgacgagat cggcgacatg gccgccgaga cccaggccaa ggtgctgcgg
     1021 gtgctggaga gcaccagcct ctaccgcgtc ggcggccgca acccggtggc gatcgacgtc
     1081 cgcttcctct cggccaccaa ccgggacctc gagtccatgg tggaggaggg gagcttccgg
     1141 cgcgacctct accaccggct ggcggcgatg gaggtgaagc tgccccccct gcgcgagcgc
     1201 cgggaggaga tccccgcgct cgccgcccgc ttcttccacc gcgagttgga gaggaacggg
     1261 gtcgccagcc ccggcatcaa ccgcgccgcc ctgggcgcgc tcgtccagta cccctggccg
     1321 ggcaacgtcc gcgagctgca gaacgagatc gcccgcgcgg cgctgctgct ggagcccggc
     1381 gagcccctgg acatccgcca cctctcccgg cgggtgcgcg gcgctgtggc ggggggcgtg
     1441 cccccggtcc aggccctgac cctggacgag accgtcgccc gcgccgagcg cgaggccttc
     1501 cgcgtcgccc tggccgccgc cggaggggac gccgcccgcg ccatggagct gctgggggtc
     1561 tcccgcacca cctactaccg caagctcaag gagctcggcc tcggggagtc cgggggctcc
     1621 ggctgaaata cccgggctcc cggagaggtt gaaggagtgg acatcaaggc tggaattgga
     1681 cgcccgagcg ggctcatccg tacaatatag gtagagaacc acggagtcga agctcgacgt
     1741 gacgcacaca aaacgccctg ccctgtccct cttcgccgct ctcctcctga gcctctgtgc
     1801 caccggcgcg gcccatgccg agctggtggt cctcaccgac ggcaacgtga tgaaggtctc
     1861 ggcctacgag gtccaggccg agcaggtgag cgtgaccctc ggccgcggcg gccgcctgac
     1921 cttccccatc acgcgggtgg agcggatcgt ggacgacgag atcgtgccgc agccggagcc
     1981 cgtgccggag gtcaccgccg aggcggaggc ggtggcctcg ctcatccccc tggagttcga
     2041 cgagtcgcag ccggtgcctg aggggcgcta cggcacgctg atttacgaga cggcgcgcaa
     2101 gcactcgatc aacccgcaga tcgtcgcggc gatgatccgc caggaatcgg cggggaaccc
     2161 gcgggcgctg tcgcacaagg gggcgcgggg gctgatgcag ctcatgcctg ccaccgccca
     2221 ccgcttcggg gtgcgcaagg agaagctcta tgagcccgag cacaacctgg aggcgggcgt
     2281 ccgctacctc acttggctga tcgaccactt ccagggcgat ctcgccaagg tcctcgccgc
     2341 ctacaacgcc ggcgagggcg ccgtcgcccg ccacggcggc atccccccgt acaaagagac
     2401 ccgcaactac gtcaagcgca tcttcggcaa cctcggcctt tcggtcgccg gcctgccgct
     2461 gtaggcgatc cgccccgcgg agcccgcgcc cggctcctcg gggcgcatag ccggcgccgt
     2521 gcggcgcgtg gccggcttgg tgcggcgcgt gagcggccgc gcgcggcgcg tcgcaggccg
     2581 cgcggagcct gtcgcgggct ccgcggggcg cgtgatgggc aaggtggcgc ccttcagagg
     2641 cgccgcgatg cgcgtgacgc gcttcgcggt gcccgccgcg ggtatcgcgg cgcgcgcgac
     2701 cggcgccgcg ccggccatga gtcagccgcg gtagccgagc accagcatcg tccggtcgtc
     2761 gctcggcggc tgaccgccgg cgtggcgggc gacggccgtg acgagccggt cccgcagctc
     2821 ctccggccgg gcggggtggg tgcccgagag cgcgctcacc acgctctcgt agccgaaggg
     2881 ctcgccggtc tcgttggggg cctcgaccag gccgtcggag agccagacca ggtagtcccc
     2941 gggccggagg tcgacggcgg cgcggccgta ggtcttcccc agggcgccga gcggggagcc
     3001 ggggagcatg atctcggtgg tccgcccgtc gcggatcaga taggtcggcg gatggccggc
     3061 gttgatgagc tgcagccggc cggccgccag gtccacgagg gccagggtgg cggtgacgaa
     3121 gaagcgccgc tcgccgttgg tccgcaccac gtcgtccagc cgccggagga tctcctccgg
     3181 gtcccgggtc tcgctcacta gcacccccag ggccgccttg agcatc
//
LOCUS       DASPJI010000057         9859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_575491, whole genome shotgun sequence.
ACCESSION   DASPJI010000057 DASPJI010000000
VERSION     DASPJI010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9859)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575491"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="VEL74_01450"
     CDS             <1..1469
                     /locus_tag="VEL74_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYG61219.1"
                     /translation="STIPREMKTATLLSLLLLPLLLLGCGPDEEGAGTAAPAASRPTQ
                     ADNAPRHVFFITVDTLRADHMSAYGYGRETSPRLDRLGASGVVFDQAIAQWPKTGSSF
                     AAVFTGRYPQTTGLTHKAALRIPAEYLTLPELFKENGFTTLAVNSNAVLALDFGWNTG
                     FDEYLQTWGYGDFPRDPRKFRQFAHAGKVNELALPLLEKHRKAERLFVWLHYSDPHAP
                     YMLPDGVENPFLNDSLFKGDETVPRKVTRGQPLDGRTDLKYYVAQYDANILVTDVAIQ
                     AVLDRIRQLGLLDDSLIVFTADHGESLGEHDSWFEHGPLPYNTTAHVPLFVVGRGLPA
                     GRRVGSPVELVDLYPTLRDLFFPGRKVEGLEGNSLVPLLAPGEPGDAARAGFCCAFSE
                     AGRRPNYFQSVQDGGWKLIYNEHRRRDDPARPGGFELYDLKADPLETKNLATAKAEEV
                     RRLRKQLFAWRKSPRSDASEDGDDAETRKALEALGYIN"
     gene            complement(1466..2638)
                     /locus_tag="VEL74_01455"
     CDS             complement(1466..2638)
                     /locus_tag="VEL74_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HYG61220.1"
                     /translation="MSCSRTLAIVGLAAALALPAAAQTTRPTRYIAFGDSITEGAGDD
                     PSRAPLTGYPPRLEALLVNAGINADVINQGLGGEATPEALSRLTGALAGTQDDDVLLL
                     MEGTNDISRGVPLEATRYNLNEMARRAEARGLTVIHATPLPRRPDARVDSNNILTLIL
                     AQNVRDMAGRRGRDLVDIFEIFITRPNRFPALYYTTTGDGVGHPNAAGYDLIAQSFFE
                     VITDVDRVSPVTGIMSPLPGAERVPAATSIQMDVWDFGAGIDVANTQMLVNGTPVAAT
                     VSGDQNRVQLAYQPPELLRGVVRVGLRSRDLALPTQNTVDREIARFVINGTKFLKGDL
                     NEDGRVDGADLVRLAVLFGARRGDNRFLPSVDLNRDDAIDGQDLAILAADFGKTSF"
     gene            complement(2635..3690)
                     /locus_tag="VEL74_01460"
     CDS             complement(2635..3690)
                     /locus_tag="VEL74_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61221.1"
                     /translation="MRELAPRTPFGALCLFVLSAIPCLAQTDSLQATRAGETWVLSTS
                     GPSARIETPAGQSLSFGLPAASEISSFVALDHGWALAGSVQDPGDGGRRRLFLMTGGD
                     TGAPRALAEPAGQSGPERRFPVLLADGGKLAGLAWLEGNDVGSFTVRAAAWTGRRFRA
                     PEAVSQPGPGSQMGLSGAVLGDGSWLLAWSAFDGVDNEIVWSRRLGGQLDGHWLPARP
                     VSTDNAVPDITPAVVATRGGALLAWSRYEEEGYRLRLVRFEGGEWREERAGGPSGSLY
                     PSWTAGADRPQLLYFTALPRGWVAAELDGSGKTVREAAVVAPYAERPAIVGSGENVEM
                     RWPARRKEAAAAWRVTQ"
     gene            3766..5265
                     /locus_tag="VEL74_01465"
     CDS             3766..5265
                     /locus_tag="VEL74_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYG61222.1"
                     /translation="MDWNSIRGWSLALAAGVGVLAIGASACSPKAEEQAVPAHRGPIV
                     LITIDALRADSLGALGGPPKLTPAMDSLAREATWAGRAISPSSWTVPSMASLFTGLQP
                     WSNQSWHGDRAVLADEFVTLPEALKAAGFRTSAFRSNHWLQQQYGYAQGFDTFRYLKE
                     GKRAEEHLAKLQGDQEFVWIHILPPHAPYVRREHLLAHLPEVPEDLPRRIRPLDLEPY
                     YDPEVPLPDDEERVFRAMYHLNVAFADEMLGRMLASLRRSGQWDKTLLVVTSDHGEEF
                     DEQGQIAHGGNLGRQLIEVPLLVKLPTGFERPLAVQPGQHPATVRLWSTLVEAAGGTP
                     RPGTAPSLFREAETGVLSELYMGNGVNRYSWVDGGRQLVWESRFAPDEPEYYRARYQG
                     IGGKPEPPLQEPMDAIFGRLEDAFVQTPPLSGRRGTKPQLTLWSWSGSGSERLDDPQL
                     TATLARQLRKAWIAANGEETLPGRGTGEKPELSEQELEELKALGYVAGN"
     gene            5354..5491
                     /locus_tag="VEL74_01470"
     CDS             5354..5491
                     /locus_tag="VEL74_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61223.1"
                     /translation="MDWQSVTVSQVCPHWDWASHWLHVQTVTSMEVCVEQVRSGSIAQ
                     E"
     gene            complement(5708..5914)
                     /locus_tag="VEL74_01475"
     CDS             complement(5708..5914)
                     /locus_tag="VEL74_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61224.1"
                     /translation="MNLPKLARIVALCLIVSATAIASFAAPPPPKPCTCEYCSTGSLG
                     KSCVWNGATIGCGEFLSFTTCVAG"
     gene            complement(5994..6191)
                     /locus_tag="VEL74_01480"
     CDS             complement(5994..6191)
                     /locus_tag="VEL74_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61225.1"
                     /translation="MNLKKFARIAALGLIVSATAMTSFAAGPKQCSCSYCPTVDPTTL
                     CKDGGVQTCGSWLAVTLCPAG"
     gene            6330..6827
                     /locus_tag="VEL74_01485"
     CDS             6330..6827
                     /locus_tag="VEL74_01485"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HYG61226.1"
                     /translation="MVQELQDRDVEAYVQLRRQALLDSPLAFAAAPGDDFVSSPDRVR
                     QQLRTGAAWVIFGAFRPDLVGAVGLFRGRYPKASHKAHLWGMYVEPGHRGQGIGAELL
                     GAVIRHARELPGVTWVHLGVTSAASEARRLYERAGFQVWGTEPDALRHEGRTVDEHHM
                     ALRLD"
     gene            6837..7295
                     /locus_tag="VEL74_01490"
     CDS             6837..7295
                     /locus_tag="VEL74_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61227.1"
                     /translation="MTTRSRLPVFPSVPSEPPGNAAVEIHDSVLDRLERRGSTLLATL
                     SPAYIHRSPGRPGVDPGTGWTGTAVFRFLGGEADGTAQSLPCELSEGELRHSGGVFEN
                     VIPVPLSLTGAVELHLRDFQGGQVVLVGQGLEVSLAGPGTFIEEFPGQAG"
     gene            complement(7305..9278)
                     /locus_tag="VEL74_01495"
     CDS             complement(7305..9278)
                     /locus_tag="VEL74_01495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYG61228.1"
                     /translation="MEDVRPDPPRAPGVPIAEPTQAARTGRLIGRLLLVLLVLSFGLR
                     VWDASKGLHAGRYFDERFPFRNVTSILRGEIRPRHTYYLSLSYLPQAAVMAASQGLHR
                     LTGAEALSIFSRKAADGYSPTAYMIGRGCNVVFGVLSLWVTFLIGRRIQSPWLGLLAA
                     AILAAFHRHVLSSAEFKPDILVLLLTAVTCYWAIAAAYRPSLGRFLRAGAGVGLAVSA
                     KYTGIAACFPVVGAALAAAAGMGGRRDRRSLGWLLLAGAASVVTFVALNPFLGLVLKY
                     IPRLTTGYAQRGAAEKSTHWTVFLRQVDFLVVHHGVVTSLFLLAGLLLLLWRLFRPAA
                     RDTAEWRLGWVVVLSQLLGYSLFHSAATTLFRGQNYLPVAPFSSLVAAWGMVEAWRLL
                     TRRAPALAGRRAAVAVWSLAGALLVGQQFAVVYLRVVPSNWEVARQILHERLPEIELR
                     HVAHEQVEGTLRLGGRSRALTTEVARLDQLAPALLDVTDAEVFPESRLDSPGGEFYRR
                     RLERLPEDRVLRVASRPFRSRGETVVILLHPWERDAVLGPVRFQRPEPGANHVMAALP
                     PGMAAGDVVSLILWVPRRAPGLDHLQLAPGDRRQALDLTARRKQRHRLGTPRFVLAEG
                     ETAVKVASPPRVRVHAYELEILRWHPEPQPARP"
     gene            9351..>9859
                     /locus_tag="VEL74_01500"
     CDS             9351..>9859
                     /locus_tag="VEL74_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYG61229.1"
                     /translation="MTSIVEPLRILVSPALYLPHRTGLTPDVQQLAERLAARGHRVTV
                     VCARHDPSTPLGESWENGVRVVRLWPFPLAISRGMVMPGYPWRVLKLMREHELVSVHT
                     PMLETALLARLTGRRIVCTHHGDLVLPGGLGNRLITSAMFGLYTSMAKDAAAMVCHTL
                     DYARHSSYL"
BASE COUNT         1539 a         3456 c         3506 g         1358 t
ORIGIN      
        1 gtagtaccat cccgcgcgaa atgaagaccg ccacgctgct ctccctgctc ctcctgcctc
       61 tgctcctgct cggctgcggc ccggatgagg agggggcggg gaccgcggcg ccggcggcga
      121 gccgtccgac ccaggcggac aatgccccgc ggcacgtttt cttcatcacc gtggacaccc
      181 tgcgggctga ccacatgagc gcctacggct atggccgcga gacctcgccg cggctcgacc
      241 ggctgggggc gtccggggtg gtgttcgacc aggcgatcgc ccaatggccg aagaccggct
      301 cgtccttcgc cgccgtcttc accggccggt atccgcagac caccggcctc acccacaagg
      361 cggcgctgcg catccccgcg gagtacctga cgctgccgga gctgttcaag gagaatggct
      421 tcaccacgct ggcggtcaac tccaacgcgg tgctcgccct ggacttcggc tggaacaccg
      481 gcttcgacga gtacctacag acctggggct acggcgactt cccccgggac ccgcggaagt
      541 tccggcagtt cgcccacgcg ggaaaggtca acgagttggc gctgcccctg ctggagaagc
      601 accggaaggc cgagcggctg ttcgtctggc tccactactc ggacccccac gcgccctaca
      661 tgctgcccga cggggtggaa aacccgttcc taaacgactc cctgttcaag ggtgacgaga
      721 cggtgccgcg caaggtgacc cggggccagc ccctggacgg ccgcaccgac ctcaagtact
      781 acgtggccca gtacgacgcc aacatcctgg tgaccgacgt cgccatccag gcggtgctgg
      841 accgcatccg ccagctcggg ctgttggacg actccctgat cgtcttcacc gccgaccacg
      901 gggagagcct gggggagcac gactcctggt tcgagcacgg gccgctgccc tacaacacca
      961 cggcccacgt gccgctcttc gtcgtcgggc gcgggctgcc ggccggccgc cgggtgggaa
     1021 gtccggtgga gctcgtcgac ctctatccga cgctgcggga cctgtttttc cccggccgga
     1081 aggtggaggg gctcgagggc aacagcctgg tgccgctcct ggcgccgggc gagccgggtg
     1141 acgcggcgcg ggccggcttc tgctgcgcgt tctccgaggc ggggcggcgt cccaactact
     1201 tccagagcgt gcaggacggg ggctggaagc tgatctacaa cgagcaccgc cggcgggacg
     1261 atccggcgcg gccgggcggc ttcgagctgt acgacctgaa ggccgatccg ctggagacga
     1321 agaacctggc caccgcgaag gccgaggagg tccgccgcct ccgcaagcag ctcttcgctt
     1381 ggcgcaagag cccccgctcc gacgcctccg aggacggcga cgacgcggag acccgcaagg
     1441 ccctcgaggc cctgggctac atcaactaga agctggtctt gccgaagtcg gccgcgagga
     1501 tcgccagatc ctggccgtcg atggcgtcgt cccggttgag gtcgaccgac ggcaggaacc
     1561 ggttgtcccc ccgccgggcg ccgaagagca ccgccaggcg taccaggtca gcaccgtcca
     1621 cccggccgtc ctcgttcagg tcacccttga ggaacttcgt gccgttgatg acgaagcggg
     1681 cgatctcgcg gtccaccgtg ttctgagtcg gcagcgccag gtctcgggag cgcagtccca
     1741 cccgcacgac gccgcgcagc agctccggcg gctgatatgc gagctggacc cggttctggt
     1801 cgccggaaac ggtggcagcc accggcgtgc cgttgaccag catctgggtg ttggcgacgt
     1861 cgatccccgc gccgaagtcc cagacgtcca tctggatgga cgtggcggcg ggcacccgct
     1921 ccgccccggg taggggggac atgatgccgg tcaccggcga gacccggtct acgtcggtga
     1981 tcacctcgaa aaaggactga gcgatcaggt cgtagcccgc cgcgttgggg tggcccacgc
     2041 catcgcccgt ggtggtgtag tagagggccg ggaagcggtt gggccgggtg atgaagatct
     2101 cgaagatgtc caccaggtca cggccgcggc ggcccgccat gtcgcggacg ttctgcgcca
     2161 ggatcagcgt caggatgttg ttggagtcga ccctggcgtc gggacggcgg gggagcggcg
     2221 tggcatggat caccgtcagg ccccgcgcct cggcgcggcg cgccatctcg ttgaggttgt
     2281 agcgcgtggc ctccaggggg acgccgcggg agatgtcgtt ggtcccctcc atgagcagga
     2341 gcacgtcgtc gtcctgggtg ccggcgagcg cgcccgtcag ccggctcagg gcctccggcg
     2401 tggcctcgcc gcccagcccc tggttgatca cgtcggcgtt gatgccggcg ttgaccagca
     2461 gggcctcgag ccgcggcggg tagccggtca ggggcgcccg ggaaggatcg tcgccggccc
     2521 cctcggtgat gctgtcgccg aacgcgatgt agcgcgtcgg ccgggtggtc tgggccgcgg
     2581 cgggcagggc cagagccgca gccagaccga cgatggcaag agtccgcgag cagctcactg
     2641 cgtcaccctc caggccgccg cagcctcttt ccgccgggcc ggccagcgca tctcgacgtt
     2701 ctcacccgag cccacgatcg cggggcgctc tgcgtaggga gcgacgacgg ccgcctcccg
     2761 caccgtcttg ccggagccgt cgagctctgc cgccacccag ccgcggggga gggcggtgaa
     2821 gtagaggagc tggggccggt ccgcgccggc ggtccaggac gggtagaggg agcccgaggg
     2881 gccgccagcc cgctcctccc gccattcgcc cccctcgaac cggacgaggc gcaggcgata
     2941 gccctcctcc tcgtagcggc tccaggcgag gagcgcaccg ccccgggtcg ccaccacggc
     3001 cggggtgatg tccggcacgg cgttgtccgt ggagaccggc cgcgccggca accagtgtcc
     3061 atccaactgg ccacccagcc ggcggctcca gacgatctca ttgtccacgc cgtcgaaggc
     3121 gctccaggcg aggagccacg acccgtcgcc gaggacggcg ccgctgagcc ccatctggct
     3181 gccgggaccg ggctgggaga ccgcctccgg cgcccggaag cgccggccgg tccaggccgc
     3241 ggcacggacg gtgaacgagc cgacgtcgtt cccctccagc caagcgaggc cggcgagctt
     3301 gccgccatcc gccagcagca cggggaagcg ccgctcgggg ccgctctgtc cggcaggctc
     3361 ggccagcgcg cggggagcgc ccgtgtcgcc acccgtcatc aggaagaggc ggcggcgacc
     3421 gccgtcgccg ggatcctgga cgctgccggc cagtgcccag ccgtgatcca gggccacgaa
     3481 cgaggagatc tcggaagccg cgggcaggcc gaagctcagg ctctgacccg cgggggtctc
     3541 gatccgggcg gacggcccgg aggtagacag cacccaggtc tcgccggccc gtgtggcctg
     3601 gagcgagtcc gtctgggcaa ggcaagggat ggcgctgagc acaaagaggc acagcgcgcc
     3661 gaacggcgtg cggggggcca gctctctcac gtgctgtatc ctaccgcaaa cccggggcag
     3721 gccggccaag gcccgccggg ccgcggagcc tatgtcttga atcgaatgga ttggaactct
     3781 atcaggggat ggagcctggc gctggccgcc ggcgtcgggg tgctggcgat cggcgcctcg
     3841 gcctgctccc cgaaagccga ggagcaggcg gtgccggccc atcgcggtcc catcgtcctc
     3901 atcaccatcg acgcgttgcg ggccgattcc ctcggagccc tgggcgggcc gccgaagctc
     3961 acgccggcca tggactccct ggcccgcgag gcgacctggg cggggcgggc gatctcgccg
     4021 tcgagctgga cggtgccgtc catggcctct ctgttcaccg gcctccagcc gtggagcaac
     4081 cagagctggc acggcgaccg cgcggtgctc gccgacgaat tcgtcaccct gccggaggcg
     4141 ctgaaggcgg ccggcttccg cacctcggcc ttccgctcga accactggct gcagcagcaa
     4201 tacggctatg cccagggctt cgacaccttc cgttatctga aggagggcaa gcgggcggag
     4261 gagcacctgg cgaagctcca gggggaccag gagttcgtct ggattcacat cctccccccc
     4321 cacgccccgt acgtgcggcg cgagcatctc ctcgcccacc tgccggaggt gccggaggac
     4381 ctgccgcgcc ggatccgccc tctcgacctg gagccgtact acgaccccga ggtgccgctg
     4441 ccggacgacg aggagagggt cttccgggcc atgtaccacc tcaacgtcgc cttcgccgac
     4501 gagatgctcg ggcggatgct cgcctccctg cgccgcagcg gccagtggga caagaccctg
     4561 ctggtggtga cctccgacca cggcgaggag ttcgacgagc aggggcagat cgcccacggc
     4621 ggcaacctcg ggcgccaact catcgaggta ccgctgctgg tgaagctgcc caccggcttc
     4681 gagcgcccac tcgccgttca gccggggcag cacccggcca cggtccgtct ctggtcgacg
     4741 ctggtggagg cggccggagg gacgccgcgc cccggcacgg cgccgagcct cttccgggag
     4801 gccgagaccg gcgtgctctc cgagctgtac atgggcaacg gcgtcaaccg ctactcgtgg
     4861 gtggacggcg gccgccagct cgtctgggag agccgcttcg cgccggacga gcccgagtac
     4921 taccgggccc gctaccaggg gatcggcggc aagcccgagc cgccgctgca ggaacccatg
     4981 gacgccatct tcggccgcct ggaagatgcc ttcgtccaga caccgccgct ctccggccgt
     5041 cgggggacga agccccagct cacgctatgg agctggtcag gaagtggtag cgagcggctg
     5101 gacgacccgc agctcaccgc gacgctcgcg cgccagctcc gcaaagcctg gatcgccgcc
     5161 aacggcgagg agaccctccc cggccgcggc accggcgaaa agcccgagct ctccgagcag
     5221 gagctggagg agctcaaggc gctgggatac gtggcgggga actgagcggc gccagcggac
     5281 gagccgctag gccccgcccc ctgggcagag gcagattccc atcctggtgc acaggccgcc
     5341 gaaacaatcg gcgttggatt ggcagtccgt caccgtctcg caggtctgcc cgcactggga
     5401 ttgggcgagc cattggctgc atgtccagac ggtgacctcc atggaggtct gcgtcgagca
     5461 ggtacgatcg ggctccatcg cgcaagagtg acagtcgcac tctcccggat agttggcgaa
     5521 ggacgtgacg gctgtggcga cgacgatgag acagaggctt gcgatgcggg cgactttgag
     5581 caggttcatc tttcctcctt cggctctggt atccatgcct tcatagatta gctttggagc
     5641 agacgaaggg acaagcggag agccgccccc aaggggcggc tctcgtggct tcagggctct
     5701 ctgtgtctca acccgcgacg caggtcgtga acgagaggaa ctcgccgcaa ccgatggtgg
     5761 cgccattcca gacgcagctc ttgccgaggc tgcccgtgga gcaatactcg caggtgcacg
     5821 gcttgggcgg cggcggagcg gcgaacgagg cgatggcggt ggcggacacg atcaggcaga
     5881 gggcgacgat gcgggccaat ttgggaagat tcatgaagaa ctccttcaag agagacagtc
     5941 gagccgcccc ggtccggggc ggctcgactt ttatctacgt ggttcgggtt ggcttaaccg
     6001 gcggggcaca gcgtgacagc cagccacgag ccgcaggtct ggacgccacc gtccttgcac
     6061 agggtcgtgg gatcgaccgt cgggcagtag ctgcagctgc actgcttcgg gcccgcggcg
     6121 aacgaggtca tggcggtggc cgacacgatg aggccgaggg cggcgatacg ggcaaacttc
     6181 ttgaggttca tcagtgctcc ttcacagagt tgaagagagc cgccccagat gagcggctcg
     6241 gctagttgag gcgcatagct taatgcaaat aagcctcgac gcataggtcg gctccggtgg
     6301 accgcgggtc cgagttagga ttgcggccca tggtccaaga gctccaagat cgcgacgtcg
     6361 aggcctacgt ccagctcagg cggcaggccc tgctggactc gcccctcgcc ttcgcggccg
     6421 cgccgggaga cgacttcgtc tcttccccgg acagagtgcg tcaacagctc cggaccgggg
     6481 cggcctgggt catcttcggc gccttccggc cggacctggt gggcgcggtc gggctgttcc
     6541 gcggccggta ccccaaggcc tcccacaagg cccatctgtg ggggatgtac gtagagcccg
     6601 gccatcgcgg ccaggggatc ggggccgagc tcctcggcgc ggtgatccgc cacgcgcggg
     6661 agctgcccgg agtgacgtgg gtgcacctcg gcgtgacctc cgcggcgtcc gaggcccggc
     6721 ggctctacga acgcgccggg ttccaggtgt gggggaccga gccggacgcc ctgcgccacg
     6781 aggggcggac ggtcgacgag caccacatgg ccctgcggct cgattaggag gacaggatga
     6841 ccacccgctc ccggcttccc gtgttcccga gcgtgcccag cgagccccct ggcaacgccg
     6901 ccgtcgagat ccacgactcg gtgctcgacc gcctggagcg ccggggctcg accctgctgg
     6961 cgacgctgtc gccggcctac atccaccgct cgccgggccg gccgggagtc gacccgggga
     7021 ccggctggac cggcacggcc gtcttccgtt tcctcggcgg cgaggcggac ggcaccgcgc
     7081 agtccctccc ctgcgagctg agcgaaggcg agctccggca ctccggcggc gtcttcgaga
     7141 acgtcatccc cgtccccctc tccctgaccg gagccgtgga gctccacctg cgggattttc
     7201 agggcggaca ggtggtcctg gtggggcagg ggctggaggt ctcgctggcg ggcccgggga
     7261 cgttcatcga ggagtttccg gggcaggccg gttagggcgg agggtcaagg acgggccggc
     7321 tgcggctcgg ggtgccagcg gaggatttcg agctcgtagg catggacgcg gacgcgcggc
     7381 ggggacgcca ccttcaccgc cgtctccccc tcggccagca cgaaacgcgg cgtccccagg
     7441 cggtgccgct gcttgcgccg ggccgtcaaa tccaaggcct gccggcgatc cccgggagcg
     7501 agctgcaggt ggtccaggcc cggggcacgc cggggaaccc agaggatcag cgagaccacg
     7561 tcgcccgccg ccatccccgg agggagcgcc gccatcacgt ggttcgcccc gggctccggc
     7621 cgttggaagc gcaccggccc caggaccgcg tcccgttccc aggggtggag caggatcacc
     7681 accgtctcgc cgcggctgcg gaacggccgg ctcgccaccc ggaggacccg gtcctcaggg
     7741 agccgttcca gccggcggcg atagaactcg ccacccgggc tgtccaggcg gctctccggg
     7801 aagacctcgg cgtcggtgac atcgaggagc gccggagcga gctggtcgag gcgagccact
     7861 tcggtggtca gcgcgcggct cctgccgccc agccgcagcg ttccctccac ctgttcatga
     7921 gccacgtgcc gcaactcgat ctcgggcagc cgctcgtgca ggatctgccg cgccacctcc
     7981 cagttgctgg ggaccacccg aagatagacg acggcgaact gctggccgac gaggagcgcg
     8041 ccggccagcg accagaccgc cacggcggcg cgccggcccg ccagcgccgg cgcacggcgg
     8101 gtcagcagcc gccacgcctc caccatgccc cacgcggcga ccagcgacga gaagggggcg
     8161 accggcaggt agttctggcc gcggaagagc gtggtcgccg ccgagtggaa gagcgagtag
     8221 cccaggagct gactcagcac caccacccag ccgagccgcc actccgccgt gtcccgggcg
     8281 gcgggccgga agagacgcca gagcaggagc aggaggccgg ccagcaggaa gagggaggtc
     8341 accaccccgt gatggaccac gaggaaatcg acctggcgga ggaagacggt ccagtgagtg
     8401 ctcttctccg ccgccccgcg ctgcgcgtaa cctgtcgtca gccgcgggat gtatttgagg
     8461 accaggccga ggaacgggtt gagggccacg aaggtgacga cggaggcggc gccggcgagg
     8521 agcagccagc ccagagagcg ccggtcgcgc cggcctccca tcccggccgc ggccgccagc
     8581 gcggcgccga cgactggaaa acaggcggcg atgccggtgt acttggccga caccgccagc
     8641 cccacgccgg cccccgcccg cagaaagcgc cccaacgacg gccggtaggc ggcggcgatg
     8701 gcccagtagc aggtgacggc ggtgagcagg agcaccagga tatccggctt gaactccgcc
     8761 gagctcagca cgtgccggtg gaacgccgcc aggatcgccg ccgccaacag gcccagccag
     8821 ggggattgga tccgccgtcc gatgaggaag gtcacccaca gggagaggac gccgaacacc
     8881 acgttgcagc cgcggccgat catgtacgcc gtgggcgagt agccatccgc ggccttccgc
     8941 gagaagatcg acagcgcttc ggcgccggtg agccggtgca gcccctggga ggccgccatc
     9001 accgcggcct gcggcagata ggagaggctc aggtaatagg tgtgccgcgg ccggatctcc
     9061 ccccggagga tgctggtcac gttgcggaac gggaagcgct cgtcgaagta gcggccggcg
     9121 tgcagcccct tgctcgcgtc ccagacccgc agcccgaagc tcaacacgag gaggaccagc
     9181 agcaggcggc cgatcaggcg gcccgtccga gccgcctgtg tcggctccgc aatgggcacg
     9241 ccgggcgcgc ggggaggatc gggacggacg tcttccatcg agtgctatgc ctgttgtcgc
     9301 ggatgaaggg ggagatacct agccgctatt cggcgcggga taatagcacg gtgacctcca
     9361 tcgtcgagcc gctgcgcatc ctggtctccc ctgctctata cctgccgcac cggacgggtc
     9421 tgacccccga cgtgcagcag ctcgcggagc gcctggcggc ccgggggcac cgggtgaccg
     9481 tggtctgcgc ccgccacgac ccctcgacgc cgctcgggga gtcgtgggag aacggcgtcc
     9541 gggtggtccg cctctggccg ttccccctgg ccatcagccg cggcatggtg atgcctggct
     9601 acccgtggcg ggtgttgaag ctgatgcggg agcacgaact ggtgagcgtc cacacgccga
     9661 tgctggagac tgccctcctc gcccgcctca ccggccggcg catcgtctgc acccaccacg
     9721 gcgacctggt cctgcccggc gggctgggca accggctcat cacctcggcg atgttcggcc
     9781 tctacacgtc gatggcgaag gacgccgcgg ccatggtctg ccacaccctg gactacgccc
     9841 ggcactcctc ctacctgag
//
LOCUS       DASPJI010000058         3886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_62076, whole genome shotgun sequence.
ACCESSION   DASPJI010000058 DASPJI010000000
VERSION     DASPJI010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62076"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="VEL74_01505"
     CDS             <1..1064
                     /locus_tag="VEL74_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYG61230.1"
                     /translation="GFDPANVMLMEVPLPVSRYPESAHRLDFFDRLFGSLRELPGVSS
                     AAVAGDLPLDDNPYIPEIKIEGREPRDPGEFQAAFNVVSDDYFRTLGIPLLQGRSFVA
                     TDRGEETLPVAIVNRSMAHRFWPGENPVGRRFEVPSHMPGQLLTVVGVVGDVRQQGLE
                     TESGPSFYLPFSQMPAGGQVFVRASFISGTLLRDVRALVRRLDPEQPVVKMQTLEQVQ
                     DEWIAPSRLTAALVGLFAILASVIAALGIGSVVAFAVAERTHEIGIRAALGASPRQLL
                     GLVVQRTLPLLVVGLILGGLGSLWLTRLMSGLLFGVEPGDPVNLAAVALAFAVVFVIA
                     TLLPARRAARIDPLLALKE"
     gene            complement(1104..>3886)
                     /locus_tag="VEL74_01510"
     CDS             complement(1104..>3886)
                     /locus_tag="VEL74_01510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61231.1"
                     /translation="RTTAKFDLTLGLREWNSQLIGAVEYVTELFDAATVDRLAGHFER
                     LLAAAVAAPDTAVYELALLSPAEREQILVEWNETGPVSEPRACLHELFAAQARRTPEA
                     VALVVGERELRYRELREAADRLAAHLRDAGVGPETVVGLCLERSTEMVVAVLAILAAG
                     GAYLPLDPRQPRQRLAGMLAASGAVALVSDERLAAELAWSGSLILVDRDSEESRDGSD
                     PVRTADPENLAYVLYTSGSTGAPKGVAVTHRSAVELVRWAGGVFGPEELSGVLAATSL
                     GFDLSVFELFVPLCYGGTVILAQDVLELPALPARERVSLVNTVPSALSELLHAGSLGE
                     SIRTVNLAGEALPRSLADRLYATGTVERVWNLYGPSEDTTYSTFSLVCRDGSGAPGIG
                     RPITATRAYILAPGLAPVPVGAAGELCLGGAGLTRGYLCRPDLTAERFVPDPFDGSGG
                     RLYRTGDFVRWLPDGTLEFLGRLDHQVKVRGFRIELGEIEAALATLAGVREAVVVARE
                     DAPGDRRLVAYVTGDAAKLAADELRQSLRERLPDYMVPTAFVILPAFPLTSNGKVDRR
                     ALARLEPERQAASWVPPRDLTELRIARLFEELLAAGPVGAGDDFFELGGHSLLAVRLL
                     ARLRRELGRDLPLSALFRGSTVERLAAAVRSGEVEGSPLVALRTGGALPPLFLVHPVG
                     GNVLCYAELARRLDPGRPVSGLQSMAAGNGHAPTLESMAISYLSEIRKVQPHGPYSLG
                     GWSMGGAVAFEMARQLSLAGETVAPLVLIDAFAPRAGGDSQPLAAADLLALFGRDLAG
                     LTGRSLGLPDTLPDESAESHLRRVHERGLAAGLFPPDLGADDVVRLFAVFRANLKAMS
                     GYRGGSYGGPLLLLRAGEGVSGAAADLGWGRLATGPLEIVDLPGDHYSILRSPAVDEL
                     SSRITAVLGAQS"
BASE COUNT          618 a         1380 c         1350 g          538 t
ORIGIN      
        1 cgggcttcga tccggctaac gtgatgctca tggaagtgcc gctgccggtc agccggtacc
       61 ccgagagcgc gcacaggctg gacttcttcg atcgcctctt cggctcgctg cgcgagctgc
      121 cgggggtgag ctcggcagcc gttgccggcg atttgccgct cgacgacaac ccttacatcc
      181 cggagatcaa gatcgaaggt cgtgagccgc gcgatccggg agagttccag gcggcgttca
      241 acgtcgtcag cgacgactac ttccgcaccc tcggcatccc cttgctccag gggcggagct
      301 tcgtcgccac cgatcgcggc gaagagacgc tgccggtcgc gatcgtgaac cggtccatgg
      361 cgcaccgctt ctggccaggg gagaacccgg taggccggcg gttcgaagta ccatcccata
      421 tgcccggcca attattgacg gtggtcggag tggtcgggga cgtgaggcaa cagggcctcg
      481 aaaccgagag cggcccctcc ttctatctgc cgttcagcca aatgcccgcc ggtgggcagg
      541 tattcgtgcg cgcctccttc atctccggca ccctgctgcg cgacgtgcgc gctctggtcc
      601 ggagactcga cccggagcag ccggtggtaa agatgcagac tctggagcag gtgcaggatg
      661 aatggatcgc cccgtcccgc ctcaccgcgg ccctggtcgg gctcttcgcc atcctcgcct
      721 ctgtcatcgc cgctctcggt atcggcagcg tagtggcctt cgcggtcgcc gagcgcactc
      781 atgagatcgg catccgcgcg gcactgggag cctcgccccg tcagctcctc gggctggtcg
      841 tgcagcggac gctgccgctg ctcgtggtgg ggctgatcct aggcggcctg ggctccctct
      901 ggctgacccg cctgatgagc ggtctgctgt tcggcgtcga gcccggcgat ccggtcaacc
      961 tggcggccgt tgccctcgcc ttcgccgtgg tgttcgtaat cgccactctg ctgccggcac
     1021 ggcgggcggc gcggatcgac ccgctcctgg cgctcaagga gtgacggttc cggaccggct
     1081 cacccccttg cgcaggcgac gcgtcagctc tgtgcaccga ggacagccgt gatccggctc
     1141 gacagctcgt cgacagccgg agagcggagg atcgaatagt gatctcccgg cagatcgacg
     1201 atctccagtg ggcccgtggc cagacgcccc cagcccaggt ccgccgcggc ccctgacact
     1261 ccttcaccgg cgcgaaggag caacagcggt ccgccatagg atccaccgcg atacccgctc
     1321 atcgccttga ggttggcgcg gaacacggca aagagccgga cgacgtcgtc cgcgccgagg
     1381 tcggggggga agaggccggc cgcgagcccg cgctcgtgga cgcgccggag gtgagattcg
     1441 gcgctctcgt cgggcagcgt gtcgggcagg ccgagggagc gcccggtgag accggccaga
     1501 tcgcggccga agagggcgag caggtcggcc gctgccaggg gctgcgagtc tcctccggca
     1561 cgcggagcga aggcatcgat gaggaccagc ggcgccactg tctcgccggc aagggagagc
     1621 tggcgggcca tttcgaaggc gacggctccg cccatggacc agccgcctag tgagtaaggg
     1681 ccgtgcggct ggactttccg gatctccgac agataggaga tcgccatgga ctcgagggtc
     1741 ggcgcgtgac cgttgccggc cgccatcgac tggagcccag agaccggacg gccggggtcc
     1801 agccggcggg cgagctcggc gtagcagagc acgttgcccc ccaccggatg gacgaggaag
     1861 agcggaggca gcgcgccgcc agtccggaga gcgacgagcg gggagccttc cacctctccg
     1921 gagcgcaccg ccgccgccag ccgttccacc gtggagccgc ggaacagggc cgagaggggc
     1981 aggtcacggc ccagctcgcg gcgcaggcgc gccagcaggc ggacggcaag cagggaatgg
     2041 cctccgagct cgaagaaatc gtcgccggcg cccaccggac cggcggccag cagctcctcg
     2101 aagaggcggg cgatccgcag ctccgtgagg tcgcgcggcg ggacccacga cgcggcctgt
     2161 cgctcgggct ccagccgcgc caacgcccgg cgatccacct tgccgttaga ggtgagtggg
     2221 aaggccggca ggatcacgaa ggcggtgggc accatgtagt ccggcaggcg ttcccgcaat
     2281 gactggcgca actcgtcggc cgccaacttg gcggcgtcgc cggtcacata ggcgaccagg
     2341 cgccggtcgc cgggcgcgtc ctcgcgcgcc actacgaccg cctcgcggac tccggcgagt
     2401 gtagccagag ccgcttcgat ctcccctagc tcgatacgga aacctcgcac cttgacctga
     2461 tggtccaaac ggccgaggaa ctccagagtg ccgtcaggca gccagcgcac gaagtcgccc
     2521 gtgcggtaca acctgccgcc cgagccgtcg aacggatcgg gaacgaaacg ctccgcggtc
     2581 agatcgggcc ggcacagata gccgcgcgtc agccccgcgc cgcccaggca cagctcgccc
     2641 gccgcaccca ccggcaccgg cgccagaccc ggcgccagga tgtaggcccg tgtggccgtg
     2701 atcggccggc cgataccggg agccccggag ccgtctcggc agaccagcga gaacgtcgag
     2761 taagttgtgt cctcggaggg gccgtagagg ttccacaccc gctcgacggt gccggtagcg
     2821 taaagccggt cggccaggga gcgcggcagc gcctcgccgg ccagattcac cgtgcggatg
     2881 gattcgccca gactccctgc gtgtagtagt tccgacaggg ccgacgggac agtgttgacc
     2941 aggctcaccc gctcccgcgc cggcagcgcc ggcagctcca ggacgtcctg cgccaggatc
     3001 accgtgccgc catagcacag cgggacgaac agctcgaaca ccgacaggtc gaagcccaat
     3061 gacgtggcgg cgagcacgcc ggacagctcc tccgggccga agaccccgcc ggcccagcgc
     3121 accagctcga ccgcgctgcg gtgggtgacg gccactccct tcggggcgcc ggtcgagccc
     3181 gaggtgtaga gcacgtaggc caggttctcc ggatcggcgg tgcggaccgg gtcgctcccg
     3241 tcccggctct cttccgaatc gcgatcgacc aggatcagcg atccgctcca ggccaactcg
     3301 gctgccagtc tctcgtcgct gaccagggcc acggcccccg aggctgcgag catgccggcc
     3361 agacgctgac gaggctgccg cggatcgagc ggcagatagg cgcctccggc cgcgaggatg
     3421 gccagcacgg cgacgaccat ctccgtcgag cgctcgaggc agaggccgac caccgtctcc
     3481 ggcccgacgc cggcgtcgcg caggtgagcg gccagccggt cggccgcttc gcggagctcg
     3541 cggtagcgga gctctcgctc gccgacgacg agggccacag cctccggagt ccggcgcgcc
     3601 tgcgccgcga acaactcgtg caggcaggcg cggggctccg aaaccggccc cgtctcgttc
     3661 cattcgacca ggatctgctc gcgttcggcc gggctcaaca gcgccagctc gtagactgcc
     3721 gtgtccggcg ccgccacggc cgccgcgagc agccgctcga agtgcccggc caagcggtcg
     3781 accgtcgcgg cgtcaaacag ctcggtgacg tactcgaccg caccgatcaa ctggctgttc
     3841 cactctcgga ggccgagtgt gagatcgaac ttcgccgtcg tccgct
//
LOCUS       DASPJI010000059         2986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_292113, whole genome shotgun sequence.
ACCESSION   DASPJI010000059 DASPJI010000000
VERSION     DASPJI010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292113"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2986
                     /locus_tag="VEL74_01515"
     CDS             <1..>2986
                     /locus_tag="VEL74_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61232.1"
                     /translation="RRHETLRTTFGESQGKPVQSIAPPPSLWELPLADLTALPVAARL
                     PEARRLAEEEAGRPFDLERGPVLRTTLLRLGAAHHALLLDIHHIASDGWSMGVMTREI
                     APLYAAAADRRPSPLPQLAVQYADFAVWQRRWLSGDVLERQLAYWRDKLAGLPVLELP
                     TDWPRPAEPTFRGVTFSHPLGAALTREARSRARQQDATLFMVLLAAFQALLERYTGQP
                     DFAVGAPIANRNRAEVEPLIGFFVNSLVLRGDLTGDPPFSGLVERTRKTALEAFSYQD
                     LPFERLVEELRPERRLTHNPLFQVAFAVQNAPVDRIDLQGLSLAPLESSSPSSRFDLE
                     VTFWEVEEELLAQITYSTDLLDEPTVRRLVGHLQSLLLGVVENPARHLSRLPVFQEEE
                     AHQILREWNDTERPLPVATVVDLFREQAARRPGAIALSSAEGDLSYADLDARSDRLAR
                     RLAGAGVRADEPVGILAERSHALVVGMLGILKAGGAYLPLDSNYPAERLAWMLADSGA
                     RVLLGQPEKLAALSETVRPQVMVELTADPALPALPADTRGPASGGILPGNLAYVMYTS
                     GSTGRPKAVGITHGNVVRLVREGGYARLGPDEVHLQFAPISFDASAFEIWAPLANGGR
                     LVMAPPGRLSLDEIGRTVESQGVTVLLLTTGLFNQMVDHRLDLLRGVRQFLTGGEALS
                     PVHGERALRGLPGVTLINVYGPTEVTVFISTHSMNRPEEVGTPVSIGRPVGNTWAVVV
                     DRELRPVPLGVIGELCAGGSGVARGYVGRPDLTAERFVPDPVGGFGERLYRTGDLARL
                     RADGLLEFLGRRDGQVKLRGFRVELGEIEAALARHPEVHEAAVTIREDWPGDRRLVAY
                     VVPRSPGGEAPVGLREALAAILPEHMIPSAFVALPALPLTSNGKVDRAALLASAAPEG
                     QAAPPVAYAPPETPLEVRIAALYAEVLGLERVGRHDGFFDLGGHSLLATQVMSRLRDS
                     LGVDLPLRRMFESSSVAGLARVV"
BASE COUNT          359 a         1052 c         1126 g          449 t
ORIGIN      
        1 gcggcgccac gagaccctgc gcaccacctt tggcgagagc cagggcaagc cggtgcagtc
       61 catcgcgccg cctccttcct tgtgggagct gccgctcgcc gacctcaccg ccctgccggt
      121 ggcggcgcgc ctgccggagg cgcggcggct cgccgaggag gaggccgggc ggcccttcga
      181 cctcgagcgc ggcccggtgc tgcgtaccac gctcctgcgg ttgggcgcgg ctcatcacgc
      241 gctgctgctc gacatccatc acatcgcctc cgacggctgg tccatggggg tgatgacccg
      301 cgagatcgcg ccgctctacg ccgcggcggc ggatcgccgg ccgtctccct tgccccagct
      361 ggcggtgcag tacgccgact tcgcggtctg gcagcggcga tggctgtcgg gcgacgtgct
      421 ggagcggcag ctcgcctact ggcgcgacaa gctggcgggc ctgccggtgc tggagctccc
      481 caccgactgg ccccggccgg cggagccgac cttccgcggc gtcaccttca gccaccccct
      541 gggagccgcg ctgacgcggg aggcgcgcag ccgggcgcgc cagcaggacg ccaccctgtt
      601 catggtcctc ctcgccgcct tccaggccct gctggagcgc tacaccggcc agccggactt
      661 cgccgtgggg gcgcccatcg ccaaccgcaa ccgcgccgag gtggagccgc tgatcggctt
      721 cttcgtcaac agcctggtgc tgcgcggcga cctcacgggc gatccgccat tctctgggct
      781 ggtggagcgt acccgcaaga cggcgctcga ggccttctcc taccaggatc tccccttcga
      841 gcggctggtg gaggagctgc gccccgagcg gcgcctgacg cacaacccgc tgttccaggt
      901 cgccttcgcg gtgcagaacg cgccggtgga ccgcatcgac ctccagggac tgtcgctggc
      961 gccgctcgag agtagctccc cgtccagccg cttcgacctg gaggtgacgt tctgggaggt
     1021 ggaggaggag ctgcttgccc agatcaccta cagcaccgac ctgctggacg agccgacggt
     1081 gcggcgcctg gtgggacatc tgcagtcgct cctgctcggc gtggtggaga acccggcccg
     1141 ccacctctcc aggctgccgg tctttcagga ggaggaggcg caccagatcc tccgcgaatg
     1201 gaacgatacg gaacggccgc tgccggtggc gaccgtggtg gacctcttcc gcgagcaggc
     1261 cgcccgccgg ccgggggcca tcgccctctc gtcggccgaa ggggacctct cctacgccga
     1321 cctggacgcg cgttcggacc ggctggcccg gcggctggct ggcgcgggcg tgcgcgccga
     1381 cgagccggtg gggatcctcg ccgagcgctc gcacgcgctg gtggtgggga tgctgggcat
     1441 cctcaaggcc ggcggcgcct atctgcccct ggactccaac tatccggccg agcggctcgc
     1501 ctggatgctg gcggactccg gcgcccgcgt cctgttgggc caaccggaga agctggccgc
     1561 gctgtccgag acggtgcggc cgcaggtgat ggtggagctg acggcggacc cggcgctccc
     1621 ggcgctcccg gcggacaccc gcggccctgc ctccggcggc atcctgcccg gcaatctcgc
     1681 atacgtcatg tacacctcgg ggtccacggg acggcccaag gcggtgggca tcacacacgg
     1741 caacgtcgtg cggctggtgc gcgagggcgg ctatgcccgg ctcgggccgg acgaggtcca
     1801 cctgcagttc gcccccatct cgttcgacgc ctcggcgttc gagatctggg cgccgctggc
     1861 caacggcggg cgtctggtca tggccccacc gggccgactg tcgctcgacg agatcggccg
     1921 gacggtcgag agccaggggg tgaccgtgct gctgctgacc acgggcctgt tcaaccagat
     1981 ggtggaccac cggctggacc tcctgcgggg ggtgcggcag ttcctgaccg gtggcgaggc
     2041 gctgtcgccg gtgcacggcg agcgcgcctt gagaggcctg cccggggtga cgctgatcaa
     2101 cgtctacggg ccgaccgagg tgacggtgtt catctccacc cactcgatga accggccgga
     2161 ggaggtgggg acgccggtct ccatcggccg gccggtgggc aacacctggg cggtggtggt
     2221 ggaccgggag ctgcggccgg tgcccttggg cgtcatcggc gagctctgcg ccggcggctc
     2281 cggggtggcg cgaggctatg tgggccggcc ggacctgacg gcggagcggt tcgtgccgga
     2341 tccggtcgga ggcttcggcg agcggctgta ccggacgggt gacctggcgc gcctgcgcgc
     2401 cgacggcctg ctcgagttcc tgggccggcg tgacgggcag gtgaagctcc ggggcttccg
     2461 tgtggagctg ggggagatcg aggccgcgtt ggcccggcat ccggaggtcc atgaggcggc
     2521 ggtgacgatc cgcgaggact ggccggggga ccgccggctg gtagcctacg tggtcccccg
     2581 gagcccgggc ggagaggcgc cggtcgggtt gcgggaggcc ctggccgcga tcctgccgga
     2641 gcacatgatc ccgtcggcgt tcgtggcgct gccggcgctg ccgctgacct ccaacggcaa
     2701 ggtggaccgg gcggcgctac tggcatcggc ggcgccggaa ggacaggccg cgccgccggt
     2761 tgcctacgcg ccgccggaga cgccgctgga ggtccggatc gccgcgctct acgccgaggt
     2821 cctgggattg gagcgggtgg ggcggcacga cggcttcttc gatctgggcg ggcattcgct
     2881 gctcgccact caagtcatgt cgcgcctgcg cgactcgctc ggcgtcgatc tgccgctacg
     2941 ccggatgttc gagtcctcct ccgtggccgg cttggcgcgg gtggtg
//
LOCUS       DASPJI010000060         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_632797, whole genome shotgun sequence.
ACCESSION   DASPJI010000060 DASPJI010000000
VERSION     DASPJI010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632797"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="VEL74_01520"
     CDS             <1..1557
                     /locus_tag="VEL74_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HYG61233.1"
                     /translation="VVTSESLLDGAEAPSPPEAASDPDHLAYVIYTSGSTGRPKGVQV
                     THANVVRLMTATDPWFGFGADDVWTLFHSYAFDFSVWELWGALLHGGTLVIVPHGVSR
                     SPEAFYALVRQEKVTVLNQTPSAFHQLLWAEEVTLTREGRAAAPNLSLRYVIFGGEAL
                     DPAGLAPWFERHGEQPTVLVNMYGITETTVHVTWCHLGRDSVSAAGSGIGRPIQDLEI
                     YLLDAARRLVSAGMAGEIHVGGPGLAYGYLDRPMLTAERFVPHPFDPREPGARLYRTG
                     DLARFQEDGQLQYLGRIDHQVKVRGFRIELGEIQAVLLAHAAVRDAVVVARSEDGRAA
                     ELVAYLVLRSDSPAAPDGEAAALEAVREHLKARLPEYMVPARLVALPALPLTAHGKVD
                     RRALPELTGARLAREESFVPPATPLQTEIAAVWAEVLRLERVGIADNFFELGGHSLTA
                     TRVIVRLNEILQMDVPVRTIFLRPTIRELAESLESARSVTVSEAALDQKMEALRERIR
                     QIKASQEGAV"
     gene            1557..>2898
                     /locus_tag="VEL74_01525"
     CDS             1557..>2898
                     /locus_tag="VEL74_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG61234.1"
                     /translation="MTPALEAELRDLEAQLLLLEGLKASGKDGPASPIGRSPDEGPRP
                     LSFAQERLWLVDRMVPGSAVYNVPGAVGLAGELDEAVLERCLNEIVRRHATLRTTFET
                     VDGQAVQVEAPPRGLSLPRIDLRALPDRQREREVRRLAAAEAGRPFDLTRGPLLRTLL
                     LELAADDHVLLFTLHHIVSDGWSMGILVRELGALYGAFRRGLPSPLAELPVQYSDFAV
                     WQRRWLSGEVLEAELAFWRGRLAGLPARLELPTDRPHPAVQTFRGGSLSLTLDRDLAA
                     DLHALGLEEGATLFMVLLAALQTLLARYAGQEDFGLGTPMAGRSRPEVEGLVGFFVNT
                     LVLRADLTGDPTCRELLARVRDVTLEAYDHQHLPFEKLVEELAPERSLAYAPLFQVTF
                     ALQNAPVEPIELPGLAVRPLEAESGTAKLDLVLALGEGAAGLAGTVQYNRDLFDA"
BASE COUNT          387 a         1003 c         1060 g          448 t
ORIGIN      
        1 gtggtgacgt ccgagtccct gctcgacggc gccgaggccc catcccctcc ggaagccgcg
       61 agcgatccgg accacctggc gtacgtcatc tacacctccg gatcgaccgg ccggcccaag
      121 ggcgtgcagg tgacgcacgc caacgtcgtc cggctgatga cggcgacgga tccgtggttc
      181 ggcttcggcg cggacgacgt gtggacgctc ttccactcct acgccttcga cttctcggtc
      241 tgggagctct ggggggcctt gctgcacgga gggacgttgg tcatcgttcc gcacggggtg
      301 agccgctccc cggaggcctt ctacgcgctc gtccggcagg agaaggtcac ggtgttgaac
      361 cagacccctt cggccttcca ccagctcctc tgggccgagg aggtgaccct cacccgggaa
      421 gggcgggcgg ccgctcccaa cctctctctg cgctatgtga tcttcggcgg cgaggcgctg
      481 gatcctgccg ggctggcgcc ctggttcgag cgccatggcg agcagccgac ggtgctggtc
      541 aacatgtacg ggatcacgga gaccacggtc catgtcacct ggtgccacct gggccgcgat
      601 tccgtctcgg ccgcggggag cgggatcggg cggccgatcc aggacctcga gatctatctg
      661 ctggacgccg ccaggaggct ggtgtctgcc ggcatggcgg gcgagatcca cgttggaggc
      721 ccaggcctgg cctatggcta tctcgaccgc cccatgctga ccgcggagcg cttcgtcccg
      781 catccgttcg atcccagaga gcccggggca cgcctctatc ggacgggaga cctcgcccgg
      841 ttccaggagg acggacagct ccaatacctc gggcggatcg accatcaggt caaggtccga
      901 ggtttccgca tcgagctcgg cgagatccag gcggtgctgc tcgcgcatgc cgcggtccgc
      961 gatgcggtgg tcgtggcccg gtccgaggac gggagggcgg cggagctcgt ggcctacctg
     1021 gtcctccgct ccgacagccc cgccgcgcca gacggcgagg cggcggcgct cgaggcggtg
     1081 cgcgagcacc tcaaggcgcg gttgcccgag tacatggtgc cggcccggct cgtggcgctg
     1141 cccgccctgc cgctcaccgc gcacggcaag gtcgaccgcc gggcgctgcc cgagctgact
     1201 ggcgcacggc tggcccggga ggagagcttc gtgccccccg cgacgccgct ccagacggag
     1261 atcgccgccg tctgggccga ggtcctgcgg ctcgaacggg tggggatcgc cgacaacttc
     1321 ttcgagctgg gcgggcactc gttgaccgcc acgcgggtca tcgtccgtct caacgagatc
     1381 ctgcagatgg atgtgccggt acgcaccatc ttcctgcggc cgacgatccg ggagctcgcc
     1441 gagagcctcg agagcgccag gagcgtgacg gtgtccgagg cggcgctgga tcagaagatg
     1501 gaagcgctgc gcgagcggat ccggcagatc aaggcgagcc aggagggcgc ggtctgatga
     1561 cgccggcgct ggaagccgag ctccgggacc tggaggcgca gctccttctg ctggaggggc
     1621 tcaaggcctc cggcaaggac gggccggcgt ctcccatcgg acgctcgccg gacgaagggc
     1681 cgcggcctct ctccttcgcc caggagaggc tgtggctggt cgatcggatg gtgcccggca
     1741 gcgcggtcta caacgtcccg ggagccgtcg gcctcgcggg agagctcgac gaggcggtcc
     1801 tggagcgctg cctgaacgag atcgtccggc gtcacgccac gttgcggacc acgttcgaga
     1861 cggtggacgg ccaggccgtc caggtcgagg cgcccccccg gggcctgtca ctgccgcgga
     1921 tcgacctgcg agccctgccg gaccgccagc gggagcggga ggtccgccgt ctggctgccg
     1981 cggaggcggg ccggcccttc gatctgacgc gcggtccgct cctgcgcacc ctcctgttgg
     2041 agctcgcggc cgacgatcac gtgctgctct tcaccctgca ccacatcgtc agcgacgggt
     2101 ggtcgatggg aatcctggtg cgcgagctgg gagcgctcta tggcgccttc cgccgcggcc
     2161 tgccctcccc gctggccgag ctgccggtgc agtactccga tttcgcggtc tggcaacggc
     2221 ggtggctctc gggggaggtg ctggaggcgg agctcgcctt ctggcgcggg cggctggccg
     2281 gcctgccggc gcggctcgag ctgccgaccg accggcccca tccggccgtg cagacgttcc
     2341 gcggcggcag cctctccctg acgctggacc gggacctggc ggcggacctc cacgccctcg
     2401 gcctggagga gggggcgact ctcttcatgg tgctgctggc ggccttgcag acgctgctcg
     2461 cgcgctatgc agggcaggag gacttcgggc tggggacgcc gatggcgggc cgcagccggc
     2521 cggaggtcga ggggctggtg gggttcttcg tcaataccct ggtcctgcgc gccgatctga
     2581 ccggcgaccc gacctgccgc gagctgctcg cccgggtgcg ggatgtgacc ctggaagcct
     2641 acgaccacca gcacctgccg ttcgagaagc tggtggaaga gttggcgccg gagcgcagcc
     2701 tcgcgtacgc gccgctgttc caggtgacgt tcgccctcca gaacgccccg gtggagccga
     2761 tcgagcttcc ggggctcgcc gtgcgtcctc tggaggcgga gagcgggacg gccaagctcg
     2821 acctcgtcct agccctggga gagggcgccg cgggtcttgc cgggaccgtg caatacaacc
     2881 gcgacctgtt cgatgccg
//
LOCUS       DASPJI010000061         9626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_804847, whole genome shotgun sequence.
ACCESSION   DASPJI010000061 DASPJI010000000
VERSION     DASPJI010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804847"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VEL74_01530"
     CDS             <1..255
                     /locus_tag="VEL74_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61235.1"
                     /translation="RAVFVPPNDTDALEQAINGLIDDPWRRQELAAAARERALEYSPE
                     RMTEEYLAAYGEVLAAAGRLFPEEPAYPAYSEPLRQRMAV"
     gene            279..1427
                     /locus_tag="VEL74_01535"
     CDS             279..1427
                     /locus_tag="VEL74_01535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG61236.1"
                     /translation="MRIVFFVHSLLSDWNHGNAHFLRGVASELISRGHDVRVYEPRDA
                     WSLQNLLADHGEAAVDGFRAAYPGLDSVRYDLRHLDLDRALDDAGLVIVHEWNDHDLV
                     RRLGEHRRHGGRYRLLFHDTHHRSVTAPQDMEAYDLSHYDGVLAFGQVIRDLYLERGW
                     AERAWTWHEAADTRVFRPLDGEPPPGNDGDLVWIGNWGDDERTAELHEFLMEPVRELG
                     LRARVHGVRYPEAGRQALAAAGIDYAGWLANHEVPRAFARHRVTVHVPRRPYVQALPG
                     IPTIRPFEAMACGIPLVSAPWHDAEGLFRPGRDFLVARDGREMKRHLRELLNDPAMAR
                     ELAEAGRRAVLDRHTCAHRVDELLTIDRQLRGAPAGVRLGIPMGVGLL"
     gene            1424..2560
                     /locus_tag="VEL74_01540"
     CDS             1424..2560
                     /locus_tag="VEL74_01540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG61237.1"
                     /translation="MRSGLTISFYGSSLVSAYWNGAATYYRGILRALAERGHRVTFFE
                     PDAFERQAHRDIPDPDWARVVVYPATEDGVVRALEQGRGADLVVKASGVGVFDQLLEE
                     AVLDLRRPDGLAVFWDVDAPATLDRLETDPSDPFRPLVPRYDLVLTYGGGDPVVEAYR
                     RFGARDCVPVYNALDPSTHMPVPPDPRFAADLGFLGNRLPDREARVEQFFLEPAARLP
                     GRSFLLGGNGWGDKPMPENVRYLGHVYTRDHNAFNCTPLAVLNISRESMARYGFSPAT
                     RVFEAAGAGACLITDAWVGIEMFLEPDLEVLVARDGEEVAARLEELTPERAGRIGRAA
                     LRRILSEHTYAHRAAQVEALLDAELAAQPVTAGVSKGAGLEAFS"
     gene            2557..3660
                     /locus_tag="VEL74_01545"
     CDS             2557..3660
                     /locus_tag="VEL74_01545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG61238.1"
                     /translation="MNHQPLDIVILGLSITSSWGNGHATTYRGLVRELSRRGHRVLFL
                     ERDVPWYAENRDLPEPPYGRTVLYSSLEELKDRFAREVRSADFVMVGSYVPEGVAVGE
                     WVTRTASGATAFYDIDTPVTLAKLSRGDHEYLTPELIPCYGAYFSFTGGPILRRLERH
                     FGSPMARPLYCSVDPSLYRPEEAAPRWDLGYMGTYSDDRQPTLDRLLLEPARRLSQRH
                     RSGQGRFVVAGPQYPAEIRWPKNVKRIEHLPPRKHSRFYNEQRFTLNVTRADMIQAGF
                     SPSVRLFEAAACGTPIISDSWPGLETFFAIGEEILTARTAGDVLHYLQIPEEERRALG
                     QRARARVLAEHTAAHRAEELEGYALSLLARKAA"
     gene            3691..4467
                     /locus_tag="VEL74_01550"
     CDS             3691..4467
                     /locus_tag="VEL74_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017912651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04290 family methyltransferase"
                     /protein_id="HYG61239.1"
                     /translation="MSATTTLADEIAALGPWFHNLHLPDGTQTAPGHFLGDFPSFKWR
                     DLQAHLPEDLSGWTALDIGCNAGFYSFELARRGARVTGIDHDPHYLRQAHWAARQFGL
                     EDRVRFRHMQVYDLGRSGESYDLVLFMGVLYHLRYPLLGLDAVARCVRRLMVFQTLTM
                     PGEEVYDAPADLAIDEREPLLAPGWPKMAFLEHRLANDPTNWWAPNHACVEALLRSAG
                     MEVTGRPGHEIYLCRPSQPREEQTWIGREMRIALGIEPEG"
     gene            4867..5361
                     /locus_tag="VEL74_01555"
     CDS             4867..5361
                     /locus_tag="VEL74_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HYG61240.1"
                     /translation="MKLESLKDLYLEQLKDLYSAETQLLEALPKMAEGAAASDLRKAF
                     FDHLRQTQEQVTRLEQIFKDLGESPKGHTCEGMKGLLKEGNQMLSMKGEPEAKDAGLI
                     AAAQRVEHYEIAGYGTVRTYAELLGRDEHARLLERTLQEEEETDELLTQLAESHINEE
                     ALAG"
     gene            complement(5418..6599)
                     /locus_tag="VEL74_01560"
     CDS             complement(5418..6599)
                     /locus_tag="VEL74_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015118239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HYG61241.1"
                     /translation="MAVLEQLRDEIQEQAGADSGLTVAVYVDPVESAKAAGLRYVTDD
                     RPGIRRRKAGKSFTYIAPDGKTVRDPQELQRIKSLAIPPAWTDVWICPVRNGHLQATG
                     RDARGRKQYRYHPEWRSVRDETKFGRMIAFGDALPKIRERLEADLSLRGLPREKVLAT
                     VVKLLETTLIRIGNKEYVRQNNSFGLTTLRDGHVDIEGSKLRFEFRGKSGQEHSVEVQ
                     DRRLARVVQRCRDLPGQTLFQYLDEQGERQKISSEDVNAYLREITGEDFTAKDFRTWG
                     GTVLALAALLEIGGCESQKEANKAVVETVKRVASELGNRPATCRKFYIHPVVIDSFVE
                     GKLVEALAEVVEDSTDTEAQGGLRGLESKTLGLLRQHTTTAAPEPEKKKRRRKSGLRA
                     V"
     gene            6853..8451
                     /locus_tag="VEL74_01565"
     CDS             6853..8451
                     /locus_tag="VEL74_01565"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HYG61242.1"
                     /translation="MSIVSMSVPEPRERMRGDRFLIVSDRLPIVLGRGEEGWRAAPAA
                     GALISALSPVLRERRGVWIGWPGVTAEKMAGLRTVLTGAIQEGGYPLRPVLLSENEER
                     NCHAGFSGEVIWPLFHDCPLECNFDPSYWRAYQRVNRKFARAAAKVLSVSGAAAVSGS
                     GGPGTGFLWVHNHLLMNVGAELRRLGLRSRTAFFLHLPFPGPDMFVKLPWRERILQGL
                     LAYDQVGLQTPRDLVNFLDCVRLLTPGAELSPCADGLWSVRGRTEAGDFELRAGAFPI
                     GVDARDFAARAARPDVQERAAALSASLRDRKMILGVDRLDRSKGIPEKLRAFAEALER
                     QPELHDRVVLVQVVVPSREDLPRHTALRAEIERLVGEINGRFGRLGWTPIHYAHRALE
                     PDELLAHYRAADVMLITPLKEGMNLLAKEYCAADLDERGALVLSEFAGAASQLADGAL
                     LVNPHDARATARTLVRALRMTAAERADRMRRLRERVRREDVSWWAGAFLDAAYADAGE
                     IADIAGSVGDSPRRETSAGLRSFS"
     gene            8569..>9626
                     /locus_tag="VEL74_01570"
     CDS             8569..>9626
                     /locus_tag="VEL74_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HYG61243.1"
                     /translation="MDAPAHDSEARQHGAPVESLEEALRPGVLRIGNDGSARSGDPRA
                     LALLGGTEGSSLESLWGDIRPRLEEAGLGWQEGGPGHAALDLTSPTSPAGARLHFDLY
                     PASGGDGVLLVSDAQVAEALEADLRLSSQMRALSQISPAVAHDLRAPINAMVFNIEVL
                     KETLAAAGGLEPAMRDRQLRYVNVLKEELNRLHKSLEVFIAHTSPRGDRVEVLDLREL
                     LEELGALLVGPARKQQVQVKTELGGERAALEVNRYLLRQALLHVALAVLGPVPRQGTL
                     VIRLECQEEAVRVRIEGVGGEPAAAGSPASSGQAAQEPGFGLLFSPGGTIGPLHVARA
                     ILAAQAGGELRPAGSGAAP"
BASE COUNT         1332 a         3595 c         3279 g         1420 t
ORIGIN      
        1 cgcgcggtgt tcgtgccgcc caacgacacc gacgccctgg agcaggcgat caacggcttg
       61 atcgacgatc cctggcggcg gcaggagctg gccgcggcgg cgcgggagcg ggccctggag
      121 tacagcccgg agcgcatgac cgaggagtac ctcgccgcct acggtgaggt cctggcggcg
      181 gcaggcaggc tgttccccga ggagccggcc tacccggcgt acagcgagcc gctccgccag
      241 cgcatggccg tctgaagacg accgttcgga tcgaacgtat gcggatcgtc ttcttcgtcc
      301 attcgctgct gtcggactgg aaccacggca acgcacattt cctgcgcggc gtcgccagcg
      361 agctgatctc ccgcggccac gacgtgcgcg tctacgagcc gcgggacgcc tggagcctgc
      421 agaacctcct cgccgaccac ggcgaggcgg cggtcgacgg cttccgcgcc gcctaccccg
      481 gcctcgacag cgtccgctac gacctgcggc acctcgacct cgaccgcgcg ctggacgacg
      541 ccggcctggt gatcgtccac gagtggaacg accacgacct ggtgcggcgc ctcggcgagc
      601 accggcgcca cggcggccgc taccgcctcc tcttccacga cacccaccat cggtcggtga
      661 ccgcgcccca ggacatggag gcctacgacc tctcccacta cgacggcgtg ctcgccttcg
      721 ggcaggtgat ccgcgacctc tatctagagc gcgggtgggc cgagcgcgcc tggacctggc
      781 acgaggcggc cgacacccgg gttttccgtc ccctcgacgg cgagccgccc cccggaaacg
      841 acggggacct ggtctggatc ggcaactggg gggacgacga gcgaacggcg gagctgcacg
      901 agttcctcat ggagccggtg cgcgagctcg gcctcagggc ccgggtgcac ggcgtgcgct
      961 atcccgaggc cgggcgccag gcgctggccg cggcgggcat cgactacgcc ggctggctcg
     1021 ccaaccacga ggtgccccgc gccttcgccc gccaccgggt caccgtccac gtgccgcgcc
     1081 gcccctacgt gcaggcgctg cccgggatcc cgaccatccg cccgttcgag gcgatggcgt
     1141 gcggcatccc gttggtgtcg gccccctggc acgacgccga agggctgttc cgtcccgggc
     1201 gcgacttcct ggtcgcccgc gacggccggg agatgaagcg gcacctgcgc gagctgctga
     1261 acgacccggc gatggcgcgc gagctggcgg aggccggccg ccgcgccgtc ctcgaccgtc
     1321 acacctgcgc gcaccgagtc gacgagctgc tgaccatcga ccgccagctc cgcggcgccc
     1381 cggccggggt ccgcctgggc atccccatgg gagtcgggct gttatgagat ccggactgac
     1441 catctccttc tacggctcga gcctggtctc ggcctactgg aacggcgccg ccacctacta
     1501 ccgcggcatc ctgcgggcgc tggccgagcg cggccaccgc gtcaccttct tcgagcccga
     1561 cgccttcgag cgccaggcgc accgcgacat ccccgacccg gactgggcgc gggtggtggt
     1621 ctaccccgcc accgaggacg gcgtggtgcg ggcgctcgag caggggcgcg gcgccgacct
     1681 ggtggtcaag gcgagcggtg tcggggtctt cgaccagctc ctggaggagg cggtgctcga
     1741 cctgcgtcgc ccggacggcc tcgccgtctt ctgggacgtc gacgcgccgg cgaccctgga
     1801 ccgcctggag accgatccgt ccgacccgtt ccggccgctg gtaccgcgct acgacctggt
     1861 gctgacctac ggcggcggcg atccggtggt cgaggcctac cggcgcttcg gcgcccgcga
     1921 ctgcgtgccg gtctacaacg ccctcgaccc gtcgacccac atgccggtgc cgccggaccc
     1981 ccggttcgcg gccgacctcg gcttcctggg caaccggctg ccggaccgcg aggcgcgggt
     2041 ggagcagttc ttcctggagc cggcggcgcg gctccccggg cggagcttcc tgctcggcgg
     2101 caacggctgg ggcgacaagc cgatgccgga gaacgtgcgc tacctcgggc acgtctatac
     2161 gcgggatcac aacgctttca actgcacgcc gctcgcggtg ctcaacatca gccgcgagag
     2221 catggcgcgc tacggcttct cgccggccac ccgggtgttc gaggcggccg gggcgggggc
     2281 ctgtctgatc accgacgcgt gggtggggat cgagatgttc ctggagccgg acctggaggt
     2341 gctggtggcg cgggacgggg aggaggtggc ggcacggctt gaggagctga cgcccgagcg
     2401 ggcgggccgg atcggccggg cggccctgcg gcggatcctc tccgagcaca cctatgccca
     2461 tcgtgcggcc caggtcgagg ctctcctcga cgccgagctc gccgcgcaac ccgtgaccgc
     2521 cggcgtatcg aaaggcgccg gcctggaggc cttctcttga accaccagcc gctcgacatc
     2581 gtcatcctgg gcctctccat cacctcgtcc tggggcaacg gccacgccac cacctaccgc
     2641 ggcctggtgc gggagctctc ccgccgcggc caccgggtgc tgttcctgga gcgcgacgtc
     2701 ccctggtacg cggagaaccg ggacctcccg gagcccccct acggccgcac cgtcctctac
     2761 tccagcctcg aggagctcaa ggaccgcttc gcgcgcgagg tgcggagcgc cgatttcgtg
     2821 atggtcggct cctacgtccc cgagggggtg gcggtgggcg agtgggtgac ccgcaccgcc
     2881 tccggggcga cggccttcta tgacatcgac acgccggtca ccctcgccaa gctgtcgcgc
     2941 ggcgaccacg agtacctgac gccggagctg atcccctgct acggcgccta cttctcgttc
     3001 accggcggcc cgatcctgcg ccgcctggag cgccacttcg gctcccctat ggcgcgcccg
     3061 ctctactgct cggtcgaccc gtcgctctac cggccggagg aggccgcgcc gcgctgggac
     3121 ctgggctaca tgggcactta cagcgacgac cgccagccga ccctcgaccg gctgctgctc
     3181 gagccggccc gccgtctgag ccagcggcac cgttcgggcc aggggcgttt cgtggtcgcc
     3241 gggccgcagt acccggccga gatccgctgg ccgaagaacg tcaagcggat cgagcacctg
     3301 ccgccgcgca agcactcgcg cttctacaac gagcagcgct tcacgctgaa cgtcacccgc
     3361 gccgacatga tccaggccgg cttctccccc agcgtgcggc tgttcgaggc cgcggcctgc
     3421 ggcacgccga tcatcagcga ctcctggccg gggctcgaga ccttcttcgc catcggcgag
     3481 gagatcctga ccgcccgcac ggccggcgac gtcctccact atctgcagat ccccgaggag
     3541 gagcggcggg ccctgggaca gcgcgcccgc gcccgcgtcc tcgccgagca caccgccgcc
     3601 caccgtgccg aggagctgga ggggtacgcc ctgagtctcc tggcgcgcaa ggccgcctga
     3661 gcccctgagc catagcgacc gtccaactct atgagcgcga ccaccacgct ggccgacgaa
     3721 atcgcagccc tgggcccctg gttccacaac ctccacctgc cggacggcac ccagaccgct
     3781 cccggccact tcctggggga cttccccagc ttcaagtggc gggacctcca ggcgcacctg
     3841 ccggaggacc tgagcggctg gaccgccctg gacatcggct gcaacgcggg cttctactcc
     3901 ttcgagctcg cccgccgcgg cgcccgcgtc accggcatcg accacgaccc gcactacctg
     3961 cggcaggccc actgggccgc ccggcaattc ggcctcgagg accgggtgcg gttccgccac
     4021 atgcaggtct acgacctcgg ccgcagcggc gagtcctacg acctggtgct gttcatgggg
     4081 gtgctctacc acctgcgcta ccccctgctc gggctcgacg ccgtcgcccg ttgcgtgcgc
     4141 cggctgatgg tcttccagac gctcaccatg cccggcgagg aggtctacga tgcccccgcc
     4201 gacctcgcga tcgacgagcg ggagccgctg ctcgcccccg gctggccgaa gatggccttc
     4261 ctcgagcacc gcctggccaa cgaccccacc aactggtggg cgccgaacca cgcctgtgtc
     4321 gaggcgctgc tccgctctgc cggcatggag gtcaccggcc gccccggtca cgagatctac
     4381 ctctgccgcc cgagccagcc gcgcgaggag cagacctgga tcggccgcga gatgcgcatc
     4441 gcgctcggca tcgagcccga aggctaggcc ctcacctccg tctcccgttc ccgcgccgtc
     4501 ctcctcccgg ggcagccgtt gccccgcgag agatggccgt cctacccgac gaaacgatcg
     4561 tcctacccgg ggtagggccg tcatcccctg ggggatggcc gccgtacccc gggtaggatc
     4621 gccgtacccg gagcgatggc ctccctgcct ggaggataga cgccatccct ccgggtagcc
     4681 tcgccctacc ctgggtaaga cggccatacc cggaggtagg acggtcatct tcccggggga
     4741 tggccgtcgc cgagcggcat caatcttgcg atggctctcc tcgaacacgc tcctccgcgg
     4801 ggccgccgtt gcgacggcgg ccttcgtgga cttttcaacc cgtcaactca tcaaggagga
     4861 gttgccatga agctcgaatc cctgaaggac ctctacctcg aacagctcaa ggacctctat
     4921 agcgccgaga cgcagctgct ggaggccctg cccaagatgg ccgaaggcgc ggccgcctcg
     4981 gacctgcgca aagccttctt cgatcacctc cgccagactc aggagcaggt cacgcgcctg
     5041 gagcagatct tcaaggatct gggggagagc cccaagggtc acacctgcga gggcatgaag
     5101 ggcctcctca aggaggggaa ccagatgctc agcatgaagg gtgagcccga ggccaaggac
     5161 gccggcctga tcgccgccgc ccagcgggtg gagcactacg agatcgccgg ctacggcacc
     5221 gtccgcacct atgccgagct gctcggccgt gacgagcacg cccggctgct cgagcgcacc
     5281 ctccaggagg aggaggagac cgacgagctg ctcacccagc tcgccgagag ccacatcaac
     5341 gaagaggcgc tggccggctg aagcgaagaa cggcgccgct ctggacggcg cggcgccgtt
     5401 ccatcctctc ccacccccta caccgcccgc agcccgctct tccttcgccg cttcttcttc
     5461 tccggctctg gcgccgccgt cgtcgtgtgt tggcgcagca ggcccagcgt cttcgactcc
     5521 agcccgcgca ggccgccctg ggcttcggta tccgtcgagt cctcgacgac ttcggccagg
     5581 gcctccacca gcttgccctc cacgaagctg tcgatgacca ccgggtggat gtagaacttg
     5641 cggcaggtgg cggggcggtt gccgagctcc gaggccaccc gcttgacggt ctcgaccacc
     5701 gccttgttgg cctccttctg gctctcgcag ccgccgatct ccagcagcgc cgcgagggcg
     5761 agcaccgtgc cgccccaggt ccggaagtcc ttggccgtga agtcctcgcc ggtgatctcg
     5821 cgcaggtagg cgttgacgtc ctcggagctg atcttctggc gctccccctg ctcgtccagg
     5881 tactggaaga gcgtctggcc cgggaggtcc cggcacctct ggaccacccg ggcgagccgg
     5941 cggtcctgca cctcgaccga gtgctcctgg ccgctcttgc cgcggaactc gaaacggagc
     6001 ttcgaccctt cgatgtccac gtgcccgtcg cgcaaggtcg tgaggccgaa agagttgttc
     6061 tggcggacgt actccttgtt gccgatgcgg atcagcgtcg tctcgaggag cttgaccacc
     6121 gtcgccagca ccttctcccg cggcaggccg cgcagcgaca ggtcggcctc caaccgctcg
     6181 cggatcttcg gcagcgcgtc gccgaaggcg atcatgcggc cgaacttggt ctcgtcccgc
     6241 accgagcgcc actccgggtg gtaccggtac tgcttgcggc cgcgggcgtc gcggccggtc
     6301 gcctgcaggt ggccgttgcg gaccgggcag atccagacgt cggtccacgc cggcgggatg
     6361 gcgagcgact tgatgcgctg aagctcctgg gggtcgcgca cggtcttgcc gtcgggcgcg
     6421 atgtaggtga acgacttgcc ggccttgcgc cggcggatcc ccgggcggtc gtcggtgacg
     6481 tagcggaggc ccgccgcctt ggcgctctcc accggatcga cgtagacggc gacggtcaga
     6541 ccgctgtcgg ccccggcctg ttcctggatc tcgtctcgga gctgctcgag gactgccatg
     6601 ttccctcgtt tgtaaagaat gcatccatgc aactgtcgca aggagccgat ccgcgggctc
     6661 acccgcgccc tgccctccac gcaatgcagc gaacatgccc gaagctcccg ccgctcaagg
     6721 gtttgcaccg ggttcacacg gcggccacgg cggcggcatc cgtatggcat acaacctgca
     6781 gctagaaaga gtttccgcgg gcaggcgtct tggcccgagg tgtctgacat ccggtctttc
     6841 agcgaggggt ttatgagcat tgtctccatg tccgtgccgg agccgcggga acgcatgcgc
     6901 ggcgaccgtt tcctgatcgt ctccgaccgt ctgcccatcg tcctgggccg gggcgaggag
     6961 gggtggcgcg cggctccggc cgcgggggcg ctgatctcgg cgttgtcgcc ggtgctgcgc
     7021 gagcgccgcg gcgtctggat cggctggccc ggcgtgacgg ccgagaagat ggccgggctg
     7081 cgcacggtgc tgaccggcgc catccaggag ggcggctacc ccctccgccc cgtgctgctg
     7141 agcgagaatg aggagaggaa ctgccacgcc ggcttctcgg gcgaggtgat ctggccgctg
     7201 ttccacgatt gcccgctgga gtgcaacttc gacccctcct actggcgcgc ctaccagcgg
     7261 gtgaaccgca agttcgcgcg ggcggcggcc aaggtgctct cggtctccgg ggccgccgcc
     7321 gtctccggca gtggaggccc tggcaccggt ttcctttggg tccacaacca cctgctgatg
     7381 aacgtcggcg ccgagctgcg ccgcctgggc ctccggtccc gcaccgcctt cttcctccac
     7441 ctccccttcc ccggcccgga catgttcgtg aagctcccct ggcgggagcg catcctccag
     7501 gggctcctgg cctatgacca ggtcggcctc cagacccccc gcgacctggt gaacttcctg
     7561 gactgcgtgc ggctgctcac ccccggcgcc gagctctccc cctgcgccga cggcctctgg
     7621 agcgtccgcg ggcggacgga ggcgggggac ttcgagctgc gcgcgggcgc cttccccatc
     7681 ggcgtcgacg cccgggactt cgcggcacgg gcggcccggc cggacgtgca ggagcgcgcc
     7741 gccgccctct ccgcctcgct gcgcgaccgc aagatgatcc tcggcgtcga ccggctggac
     7801 cgctccaagg ggatccccga aaagctccgc gccttcgccg aggctctgga gcgccagccc
     7861 gagctgcacg accgggtggt gctggtccag gtcgtggtgc cgagccggga ggacctgccg
     7921 cgccacaccg ccctgcgcgc cgagatcgag cgtctggtcg gcgagatcaa cggccgcttc
     7981 ggccgcctcg gctggacgcc gatccactac gcccaccgcg cgctggagcc ggacgagctg
     8041 ctggcccact accgggccgc cgacgtgatg ctgatcacgc cgctcaagga ggggatgaac
     8101 ctgctggcca aggagtactg cgccgccgac ctcgacgagc ggggcgcgct ggtgctctcc
     8161 gagttcgccg gcgccgcctc ccaactcgcc gacggcgccc tcctggtcaa tccccacgac
     8221 gcccgcgcca ccgcccgcac cctggtacgg gccttgcgga tgacggcggc ggagcgggcg
     8281 gaccgcatgc gccggttgcg cgagcgagtc cgccgcgagg atgtctcctg gtgggctggc
     8341 gcgttcctgg acgccgccta cgccgatgcc ggcgagatcg ccgatatcgc cggctccgtc
     8401 ggcgactcgc cccgccggga gacgtcggcc ggattgcgga gcttctcctg accctccccg
     8461 cccggcgctc ttgcaccctc gagggcaagc ttctataaat agccctctgc cgccggtccc
     8521 ttccgggcac cggcacgacc catgccccaa gccggaggaa gagccgacat ggacgcgcca
     8581 gcacacgaca gcgaggccag acagcacggc gcgccggtcg agagcctcga ggaggctctg
     8641 cgacccggag tgctgaggat cgggaacgac gggagcgccc gctccggaga tccgcgggcc
     8701 ctggccctgc tcggaggcac cgaaggctcc tctctcgaga gcttgtgggg cgacatccgc
     8761 ccgcgcctgg aggaggccgg cctgggctgg caggagggcg gcccggggca cgccgcgctc
     8821 gacctgacat cgccaacgtc gccggccggc gcgcgcctgc acttcgacct gtatccggcc
     8881 agcggcggcg acggcgtgct gctggtcagc gacgcccagg tcgccgaggc gctcgaggcg
     8941 gacctccgcc tctcctcgca gatgcgggct ctgtcgcaga tctcgcccgc cgtggcgcac
     9001 gacctgcggg cgccgatcaa cgccatggtg ttcaacatcg aggtcctcaa ggagaccctc
     9061 gccgcggccg ggggactgga gccggcgatg cgggaccgcc agctccgcta cgtcaacgtc
     9121 ctcaaggaag agctgaaccg cctgcacaag tccctcgagg tgttcatcgc ccacacctcg
     9181 ccgcgcggcg atcgcgtcga ggtcctcgac ctgcgcgagc tgctggaaga gctgggggcc
     9241 ctcctcgtgg gcccggcgcg caagcagcag gtccaggtca agaccgagct gggtggggag
     9301 cgtgccgccc tggaggtgaa ccgctacctg ctgcggcagg cgctgctcca cgtggcgctg
     9361 gcggttctcg ggccggtgcc gcggcagggg acgctggtga tccgcctgga gtgccaggag
     9421 gaggcggtgc gggtccggat cgagggcgtc ggcggagagc ccgcagcggc gggatcgccg
     9481 gcttcgagcg gccaggcggc tcaggagccc gggtttgggc tactcttctc ccccggcggc
     9541 accattggcc cgctgcacgt cgcccgcgcc atcctcgccg cgcaagccgg cggcgagctg
     9601 cgccccgccg gttccggggc ggcgcc
//
LOCUS       DASPJI010000062        25786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_235303, whole genome shotgun sequence.
ACCESSION   DASPJI010000062 DASPJI010000000
VERSION     DASPJI010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 25786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25786)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25786
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235303"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..685
                     /locus_tag="VEL74_01575"
     CDS             182..685
                     /locus_tag="VEL74_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61244.1"
                     /translation="MTHAAAAARAFERTLEELERQPDSPDLGDPAALGRRAALLAVAD
                     AVWGKALGPLYDVEQVKALLEVSSRQAVSDLAKRGRLLALEASGGRKLYPAFQFGPDG
                     RPYPEIVRVLEAFAGAVESFYTIASWFVTPQDLLDGETGAAWMRSRRDPERLLEAARR
                     AAAPLAR"
     gene            767..1264
                     /locus_tag="VEL74_01580"
     CDS             767..1264
                     /locus_tag="VEL74_01580"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HYG61245.1"
                     /translation="MHRVANGPWWFSTDGSGRFDLPRGSDRGTCYLADDPLGCFLEVF
                     RFTWLVPEEEVQRRRLARLELPEVLLADCTASRSRRFGITAEIHSTTDYGLTQRWAAA
                     FAAAGFDGVRYLLRHDPGQMLSGVALFGPAGAPDWEVPPGEPLGSDLLLEAERRFGIR
                     VLPTP"
     gene            1355..1531
                     /locus_tag="VEL74_01585"
     CDS             1355..1531
                     /locus_tag="VEL74_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61246.1"
                     /translation="MKKTKKLMLNRETLRTMDVAGGDKPGTHPELPNTMVTSCDYTYS
                     CREWSCTCPVQLIY"
     gene            1664..4921
                     /locus_tag="VEL74_01590"
     CDS             1664..4921
                     /locus_tag="VEL74_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYG61247.1"
                     /translation="MKSLASALSPFFSAKLRRRGDEYFHLRLVRLLSCTGDQVEAVVS
                     GPEDYRVVLRREEKTVRASCTCPFASDGTGVCKHIWATVLAADQEGGLRGPLGGLPNR
                     VVYLSPDRDEEEGEVDLSGVPSAARPQRASPPPRLPAWREILAPIAGASPLPAARQTE
                     ILYVIDLPGSLERQRLCLEILTLQRKQDGSPGALHPLRMNRAQIAQHHEPLDRAIFQL
                     LLGAETSNPYLIRYQEVPQHPSVPNEIAPEIARRLCLTGRCRLRAQDRQIGEQPLAWY
                     DGPPWELWLVVRDEGDGGCEVVPELRQGDTRQDARGLPLLDQAGLLLTGGGVVPVDTG
                     GAAAWLPLLRRAGSLRVPAAEREDFLEVLLAAPVLPHLELPDSMRVEEVSVAPQPRLR
                     LSPPGHRSSPAGWPTATVSYLYEELEVPAGPGRRGIYQKGQRRFLLRDWEAEEQALTR
                     LASLGFRTGMPDPRGEPALRIAPSRIPQAVRALLAEGWSVEAQGKLYRTAERFDVRVT
                     SGIDWFELHGDVELGGETVGLPRLLAALRRGDGFVPLSDGSLGLLPEEWLKRWEPLAA
                     LGTAEGDHVRFEMPQALLLDAWLAAEPETSFDETFTRARERLRSFSGIVPAAAPEGFR
                     GTLRPYQQLGLAWLHFLRDFGLGGCLADDMGLGKTVQVLALLESRRQRRAAEGLPPAL
                     VVVPRSLVFNWQVEAAQFTPALRVLDYSGSGRSRDLESFAGHDVVLITYGTMRRDIGI
                     LKEAQFDYVILDEAQAIKNAASQTAKAARLLRGHHRLALTGTPIENHLGELWSLLEFL
                     NPGFFGSAFHAKPAELREAGTAARETLARALRPLILRRTKDQVAPELPPRTEQTLICD
                     LPNKQRKLYDELRDYYRAVLDQRIAAQGLGRSKLLVLEALLRLRQAACHPALLDRGHA
                     DDPCAKLDLLLPQLEEVLDEGHQALVFSQFTSFLALLRRRLDAAGLPYLYLDGKTRKR
                     QELVDAFQSRTGTRLFLISLKAGGLGLNLTAADYVYLLDPWWNPAVEAQAVDRAHRIG
                     QTRSVFAYRLVARDTVEEKILELQAGKRALAEAIIQADDSLIANLGREDLELLLS"
     gene            complement(4907..5587)
                     /locus_tag="VEL74_01595"
     CDS             complement(4907..5587)
                     /locus_tag="VEL74_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYG61248.1"
                     /translation="MSLPALLPAAAPTALSDEEVVARVLAGDTSLFEILMRRYNQRLF
                     RVARGIVTDDAEAEDVTQDAYVRAFRDLASFRGEARFSTWLTRIACHEALARARKRRR
                     LVSIGGDEPPEPPTETPGPEKELANRELHAILREAVEILPDPLRSVFCLREIEGLSTE
                     ETADALGLTVENVRVRLHRAKRSLRERLDQRIGDEVRRLYLFDGARCDRMVENVFARI
                     DRPASREQ"
     gene            complement(5609..6040)
                     /locus_tag="VEL74_01600"
     CDS             complement(5609..6040)
                     /locus_tag="VEL74_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HYG61249.1"
                     /translation="MTTTHSNPSLESLYRPLWLTYGLVPLLAGLDKFFNLLTDWPQYL
                     SPVMAGLLPVSPETFMYAVGIIEMAVGVMILTRWTRLGAWIAAAWLVLIALNLVTMGL
                     LDIAVRDLAMAVGAYTLARLAELRQEEPAAARRASLRESRA"
     gene            6119..7174
                     /locus_tag="VEL74_01605"
     CDS             6119..7174
                     /locus_tag="VEL74_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019677055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HYG61250.1"
                     /translation="MDISRRQALAGAAALAAAGALPHPLPALRSDGRPRTVRPRRLQP
                     GDTVGLVDPASAVWEPMNVEIVTESLAALGFKTKRGAHLLARRGYFAGTDEQRAADLN
                     AMFADPEVRAIQCVRGGWGSARLLSLLDWNTIARSPKILLGYSDITALLLALHAKTGL
                     VTFHGPVGLSQWNPFSVGYIKRVLQEAQAVTFENLKEIDEDDLTVVENRVQTLRPGTA
                     RGRLLGGNLTVLTSLVGSDYLPDWDGCILFLEDVEEAPYRIDRMLTQLRLAGILRKAR
                     AVIWGNCTGCRPGEGYGSLTVTDVLQDHLLPLGVPVWSGAMIGHIDRQFTLPVGLEVE
                     VDATAGTIRMLEPAVTG"
     gene            7338..7550
                     /locus_tag="VEL74_01610"
     CDS             7338..7550
                     /locus_tag="VEL74_01610"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HYG61251.1"
                     /translation="MLASSPGRRRDIHVERDHQTAKYWLEPVRLARSKGFKSQELNSI
                     RKIVEENQFILRKEWNDYFESRDSGL"
     gene            7516..7815
                     /locus_tag="VEL74_01615"
     CDS             7516..7815
                     /locus_tag="VEL74_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012266687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HYG61252.1"
                     /translation="MTTLRAEIQVSSAERVSVTEEMLTVDLNDGRVISVPLSWYPRLV
                     QGTPEERAHWRLIGHGEGIHWPDLDEDISVENLVCGHPSGESQSSLQRWLENRAR"
     gene            complement(7827..9146)
                     /locus_tag="VEL74_01620"
     CDS             complement(7827..9146)
                     /locus_tag="VEL74_01620"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018841906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HYG61253.1"
                     /translation="MRQKSWLLGLMMTWPVFTGGPAAAQAPAWQPPDAALVTRARQIL
                     DKVPLIDGHNDLPWEYRQRVRNHLDKIDLRGDTSKLEKPMHTDIPRLRRGGVGGQFWS
                     VYIPADISGPAAVKTVMEQIDVVHRLAERYPEVFEVATTADDVVRIHKAGKIASLIGM
                     EGGHSIDNSLGTLRQLYNAGARYMTITHSKNNDWADSATDGPKHGGLTRFGEEVVREM
                     NRLGMLVDLSHVSPDSMKDAMAVSEAPVIFSHSSARAVTDHPRDVPDDVLQLLKEDGG
                     VVMVTFVPSFVSEDVRQWGADEDAAEARYKALFPGDPDRAKAELEAWKKANPAPRATI
                     PQIADHIEHVRKVAGIDHVGIGSDFDGITAVPLGLEGVDRFPVLLAELLRRSWTEQDI
                     QKLAGLNVLRVMREAEKTAARLQKERPASDALIEELDGEGGEAKPAG"
     gene            9219..9545
                     /locus_tag="VEL74_01625"
     CDS             9219..9545
                     /locus_tag="VEL74_01625"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYG61254.1"
                     /translation="MDHPTPFRPEAHALYDSGKMGKATLYQSERLLVGLNAFEPGQEH
                     KLHAHAGMDKVYHVLAGQGLFLLEGREEPMEPGTMLIAPEGVPHGIRNSGAERLLVLA
                     ILAPSP"
     gene            9641..10927
                     /locus_tag="VEL74_01630"
     CDS             9641..10927
                     /locus_tag="VEL74_01630"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HYG61255.1"
                     /translation="MANIAFVLDHEEGHLLPTFKLAARLASRGHAVRYLGLADAEDFV
                     RRQGFEFSPILEDVFPLGSTKTLRERTRGQDPEEETGPIHGVLGAGEISDEYLISLIH
                     GRDLGDAVEDTRPDLFLVNSHFVLHALVLSYRFKIPVVLLTPWLRPFPKTGYTQMIET
                     SLLQLRTAGLELFSLVQSADPTARRFQDIAARFLAMRELIMCPAELELPRDGQAPPEH
                     EVYHVEASVDLARRDDRELPWERLDPNKRLLYCSVGSQSQLVGREKIARFLGAVVEAA
                     TGLAGWQLVLATGGMVGLRDLPPLPPDAIATGWVPQLPVLERASAMITHCGLGTVKEC
                     IFHGVPMLAVPMMRDQPDNAKRIVHHRLGVACDFETVTAGGIAALIRQLDEDPAIRQS
                     VARMQRRFHEIEDSGVGVQRVEEVLNGKARPAAGHG"
     gene            11092..12459
                     /locus_tag="VEL74_01635"
     CDS             11092..12459
                     /locus_tag="VEL74_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61256.1"
                     /translation="MLSVWMILSMLGAGLAWAAPGTSTPSTSRPAGNPLETLSLALAG
                     ALEDRDFRDLLRQSLAASPSGSVSLRGLLAKEVAQGSTVAQRLARGLSRTHDGSPGDR
                     GGAAVKARALHRVLGLLEDMPHVDLTVAPGLAAWDAAQVPLTTYTRDGEVTTVEAFEP
                     DGSRVTLDTRRPLPVPVIVLEHHDTSDAVEIVSRAAPDAMDLDHSGVASATAALYGQC
                     WDSTTYKSYPLITGAWIWDIHEGCCNGPEGYAYFSVAGSEKTGNLPASLGGQSTWGTN
                     VWASNFQGPKKIEKTRLQWEWIADGWMEGSNMVYPAAYCQQSATSTSGFSQVAYTIKE
                     DDDGPFSSDDYVGKVQIDHRHCKGEVFDLGLASGWSLEHMTAGIDDVEYTRYKLYCST
                     QTNCSASVECPNGGFVSCSGSGTCGQGSCVAGEDWVQCGGYFESCWSNPEPCPGGQIV
                     CEQPL"
     gene            12630..13268
                     /locus_tag="VEL74_01640"
     CDS             12630..13268
                     /locus_tag="VEL74_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61257.1"
                     /translation="MAKPFHFLAGAASDGHGFLPAVTCLEPVGTNGADDNVIHWLLYQ
                     PSQQFPDQQFAVAVAETELSGAFTNPLVMASDRALATYLQTRGFVEVEVPDDPEVKCI
                     DTKALAESAGEEQEKACADPALTNPVVQALILQTIDSQILGNDPPEARETLQRLVGQG
                     FTPDQAKNLIGAAIATEIFELYEHKASFNRERYVENLKKLPFLPERGEQTEG"
     gene            13506..13916
                     /gene="ndhC"
                     /locus_tag="VEL74_01645"
     CDS             13506..13916
                     /gene="ndhC"
                     /locus_tag="VEL74_01645"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HYG61258.1"
                     /translation="MPAQYYPVLLTLGIAILMGVVILGLSQGLAWMMGNQRGGRVKLT
                     PIESGMPLLDRSHKRLSIAFFLVAIDFIVFDLEAAFLYPWALIVREGGWELFAAVMVF
                     IFLILVGYAYVWLKGGLELRPQRERQTGSARIQP"
     gene            13913..14431
                     /gene="nuoE"
                     /locus_tag="VEL74_01650"
     CDS             13913..14431
                     /gene="nuoE"
                     /locus_tag="VEL74_01650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HYG61259.1"
                     /translation="MSVSEPATGAAPVSGHAGLPPEAKAELDEIRRRYPTNEAALLPA
                     LHIAQKVWGGWLPDEAIQAVARELDLAPAKVYGVVTFYDLYHQKPVGRHRIRVCTNLS
                     CQLRGSEEIMETLHQELGVGEDEVTPDGRVSYVHFECLGSCDTAPMMMVDETYQENLT
                     PDRVRQILKGLK"
     gene            14432..15718
                     /gene="nuoF"
                     /locus_tag="VEL74_01655"
     CDS             14432..15718
                     /gene="nuoF"
                     /locus_tag="VEL74_01655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HYG61260.1"
                     /translation="MPAFEPVLTRNVGKPEALTLKGYRQLGGYEALAKALKMEPDAII
                     EEVKKSGLRGRGGAGFSAGLKWSFIPKGKDKPKYLVCNADESEPGTFKDRLLMEYDPH
                     QLIEGCAICCYAVHATTCYIYIRGEYTKAAAILERALKEAYEGGILGDSVMGSGFKLN
                     MWVHRGAGAYICGEETGMLESLEGKRGQPRVKPPFPAVVGAFGSPTVINNVETLCNVP
                     HIIERGADWFKAIGTDERNTGPKLYAISGHVERPGTYEYPIGVTFRELLEGCGGMYKG
                     GNLKAFIPGGASAPILPAEHIDIKMDFDTVAKAGSMLGSAAVMVMDDKTCMVGSVARL
                     AKFFNHESCGQCTPCREGTNWMELVLKRLEHGEGSEADKTLLLSVCNHIGGNSLCALG
                     DAAIGPVKSLVERYPDEIDQHVREKRCPFPRRRYFS"
     gene            15734..17416
                     /locus_tag="VEL74_01660"
     CDS             15734..17416
                     /locus_tag="VEL74_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HYG61261.1"
                     /translation="MPKVTINDRTVEVEAGTTLVDAALKVGVQIPHYCYHPRLSVVGQ
                     CRMCLVKIEGMPKLQAGCSTTVVKDGMVVRTDVPEVEEAQKGMMEFFLINHPLDCPIC
                     DQAGECGLQDYSFKHGVAYSRFRYEDKRTYPGRERIPLGANVILNMNRCIQCTRCVRF
                     TQEIAKTGELGFFNRGARTEIGTFPGKELSNPLATCVVDICPVGALTSTRFRFAERVW
                     YLDKRPSVCTGCEVGCNITMEHRRGDIKRYKPRYNPDVNDYWMCDYGRATFERYKQIP
                     RMQAVRVKASAAGVAGNGNYIAAGWKEALDAVHRQLTMADGPVAFLGSPYLTTEEAWL
                     FSQLADAVGSPHRSVPVDIGPERTIPNLKGGITGREAAPNRRGAELAGLTPGTGGDLD
                     ADELILGDGAARCSVLVVCDSDFSATAYAPETVEKLRRAKTLIVFGWADSPLAKVADI
                     ALPLAHHAEKDGTFVNVEWRLQKVERAFPAPGQVRPGVDVLAELLSRFDAHWAKVTIP
                     AAFDRMAAALPAMAGLNWESLPATGAPLNVPQARPATSATSAEAANQEAGIG"
     gene            17413..18438
                     /gene="nuoH"
                     /locus_tag="VEL74_01665"
     CDS             17413..18438
                     /gene="nuoH"
                     /locus_tag="VEL74_01665"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HYG61262.1"
                     /translation="MSYVPSDLIAVVAKLAVMLGFILTVVPVMVWVERRGSALIQDRP
                     GPNRLGPLGLIQPLADALKFMFKEDVTPSAADKPLYLAAPALALLPALSTICVIPFGP
                     DLVVNGETHRLVIADVPVGVLLFLALSSLGVYSLIVAGYASNNKFSLLGGIRASAQLI
                     SYELALTVAVLAVLMPVGSFHLADVVSYQREHIWNVVPQFVGFVVFLIASFAETNRLP
                     FDLPEAESELVAGFHTEYSAMRFASFFMAEYAAMTALSALGVTLYFGGWSLPGVDLDG
                     WLGALVGLITLVVKVAICMAVFVWIRWTFPRFRYDQLMRLGWKVLLPLALLNLIVIAA
                     LTVAGWL"
     gene            18459..19076
                     /locus_tag="VEL74_01670"
     CDS             18459..19076
                     /locus_tag="VEL74_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HYG61263.1"
                     /translation="METLVFAVFAFLAVASAIAVVAHRNPVYSTMSLVVTLFSVAVLF
                     VMLGAPFLGVIQVLVYAGAIVVLFLFVIMLLNIQAEEMPQGGQMAQRIASILGAVVFA
                     GMLGLVFWRTMQPNSPPLTEELVSLRNLSRELFFEYLLAFEMIGLLLLVAVIGATVVA
                     RRPQQADTTEDWAASGDAPRVDGARSEPHINPETGDRVAARAGRS"
     gene            19080..19382
                     /gene="nuoK"
                     /locus_tag="VEL74_01675"
     CDS             19080..19382
                     /gene="nuoK"
                     /locus_tag="VEL74_01675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HYG61264.1"
                     /translation="MVPVSWYLLLSALLFAIGTAGALTRRNGISVFLSIELMLNAANL
                     SLVAFARQFDEHTGQVIVFFVMAVAAAEAAVGLALFIAVYRQRRTIDINRINLMRW"
     gene            19396..21333
                     /gene="nuoL"
                     /locus_tag="VEL74_01680"
     CDS             19396..21333
                     /gene="nuoL"
                     /locus_tag="VEL74_01680"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HYG61265.1"
                     /translation="MLELLWLIPLLPLVGAALNGLLARAPKPVTTAIGLGAPLASLVI
                     ALGCLWEFVTRLHPAGQAFRQVLYPWTAGDLNIDVAFRLDELSAVMLFVVTFVGFLIH
                     VYSVGYMSHEHGYRRYFCYLNLFMAAMLMLVLGDNFLMMFVGWEGVGLCSYLLIGFYY
                     DQAFPPFAGKKAFIVNRVGDFAFLVGMFALIERFGTLSYDGIFPVVEADPGLVTGPYV
                     LGLTFAGFVTLCFFIGAMGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGVYMVV
                     RSNVLFQLAPGVSALVAAVGAATALFAATIGLAQTDIKKVLAYSTVSQLGYMFMAAGL
                     GAYTAAVFHLGTHAFFKALLFLGSGSVIHAMSGEQDMRSMGGLKKYLPVTFWTFVIGT
                     VAIAGIPPFAGFFSKDEILHAAAADGKWLLWAVGLLTAGITAFYMFRLVFMTFFGKFR
                     GTHEQQHHLHESPPSMTIPLIVLAIGSVLAGFVNVPELLGGNEALHHFLDPVYAQPRG
                     AEAAHHEMSHGVEWALVALAVAVALAGIFLAWTIYGKKGLEGGRTWAQRFPAAHRLLA
                     NKYYVDELYDRTIVRPLAALSRFFWKVVDTFVIDGAVNAGAFLTELTGDLGRFTTTGN
                     VRNYALYFFLGVVLLFWWIAL"
     gene            21335..22945
                     /locus_tag="VEL74_01685"
     CDS             21335..22945
                     /locus_tag="VEL74_01685"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HYG61266.1"
                     /translation="MDTFLELLVGNTLTRLIFFPTLACLPLLFFPKGEGGYRAVKIYT
                     LAVSLIELALGLLYVATHMTWGGGFPLVRDGGESLDWIRSYGIRYDMAIDGISMPLVV
                     LTVILVPIVLLGSWKGIERHWRSFSASLLLLTTGMLGALLAHDLFLFYIFWEVMLVPM
                     YLLIGVWGGERRIYAAVKFFLYTMAGSLLMLIAILWMAWTYQSLNGGVWSFALADLLR
                     LELPASTQIWLFAAFALTFAIKVPMFPFHTWLPDAHVEAPTGGSVILAGVLLKLGTYG
                     FLRFAMPLFPRATEAAQPLLVALALIGIIYGALVAWVQVDMKKLVAYSSIAHLGFVML
                     GLMAIDMVAWQGALLQMVNHGLSTGALFLLVGMLYDRRHTKQFSEFGGLAKVMPLFAF
                     FLVFSMLGSVGLPALNGFVGEFLILAGSYRTLGWPAVVATFGVVLAAIYLLKMVQLTI
                     FGPITKEENRGLKDLSGREIAALVPLCVLMLWIGVAPQAFLEPSREALGRVLAEYRQS
                     LERPEVTQAAIRPAVAVQPTEQIAEAGR"
     gene            22942..24402
                     /locus_tag="VEL74_01690"
     CDS             22942..24402
                     /locus_tag="VEL74_01690"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HYG61267.1"
                     /translation="MSRGDLMSLLPEMILTGGGVLVLMLEALVPRIRRSFTGLSLLVT
                     AAAAWGAFVAWSWKPSPEPWLTWNGLVEMTGVTLALSLVVLVATALSLLASQGYLRRE
                     RILSGEYHALLLWCATGLLLMLRGTELLTIFVALELFSLCLYCLAAFHRQVNVASEAA
                     LKYFLMGAFVSAFILYGIALVYGATGSTRMAEIGQALEAVGRMPLTASLGLLLLVAGF
                     GFKLALVPFHAWSPDTYQGAPTPFAAFLSVAPKVASALVLFRVLEAAAQGGAAATSGR
                     WSGLVAILAVLSMLVGNFLALVQRDIKRMLAYSGIAHMGYLMLAMVVLDDASLPPVVV
                     YLAAYVLMNAGAFAIVTMLYSRAGEQHLISDLSGWGYRYPLLGACLTICMLSLAGIPP
                     TAGFLGKYLVFLNAVGDGRVGLAIVGVLSSLVGVFYYLRVIYVLYMKAESRQPEGLLI
                     DGWGRAAAVLSALGVLVLGIWPTGILQWLWEATGAR"
     gene            24399..25025
                     /locus_tag="VEL74_01695"
     CDS             24399..25025
                     /locus_tag="VEL74_01695"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HYG61268.1"
                     /translation="MSELEEVSPLTLNRLSFYLRCLRHLQELGIKRISSQEMARRYHL
                     SATQIRKDLAQFGEFGIRGVGYEVDELADHLNSLLGLDRKHALLIVGMGNLGSALACY
                     LGFNYGAFHVVAAVDNDPTKVGRRFGELAVRHASEMAEVVRESGAEIGVLTVPPDAAQ
                     ENYDALADAGIRGVLNFAPVRVKRRAGVPLKNVDLRINLEELAFFLRG"
     gene            25123..>25786
                     /locus_tag="VEL74_01700"
     CDS             25123..>25786
                     /locus_tag="VEL74_01700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYG61269.1"
                     /translation="MGHVSNDGLGHSFAFAEGSWRINPNHLLFEPLGAWWQGLWTRID
                     PDRAPVDALKLLSGLSGALAAGLFRWGVAGRLAESRWAANHATAWLAFSSAFLRLWIS
                     DEIHMIQMPFVVAAAVMALRYLERPALSRALATGAAVGLAGLGFISNLLLGPALALGL
                     AWWHLRRRDARSAVTAIAGIGVGSAVVAGSAFLAVWASSPAPVGFVDWLTRYGGGAPG
                     ERV"
BASE COUNT         3690 a         9241 c         8603 g         4252 t
ORIGIN      
        1 gacccaggtt ccggagaggt gacgtgccgc ccctgccggg gctggggggg ttggagcgac
       61 cggttcctgg ggctcacgcc gccaggctaa gttctttcgc cgcttcgcgg ctgacacgac
      121 cccagagctt ccctctcttg acgctcttga cttgacagtc ttgacgctct aggctgtccc
      181 catgacgcac gccgccgctg ccgcccgcgc tttcgagagg accctggaag agctggagcg
      241 ccagccggat agcccggacc tgggcgatcc cgcggctctc ggccggcgcg ccgccctgct
      301 cgccgtggcg gacgcggtct ggggcaaggc gcttggtccg ctctacgatg tcgagcaggt
      361 gaaggcgctc ctcgaagtga gcagccggca ggcggtgagc gatctggcca agcgcggccg
      421 gctcctggcg ctggaggcgt ccggcggccg caagctctac cccgccttcc agttcggacc
      481 ggacggccgc ccttatcccg agatcgtccg ggtgctcgag gccttcgccg gcgcggtcga
      541 gagcttctat accatcgcct cctggtttgt cacgccccag gatctcctcg acggagagac
      601 cggcgcggcc tggatgcgat cgcgtcgcga ccctgagcgc ctcctcgaag cggcccgccg
      661 cgccgctgcc cccctcgccc ggtaagccct ggacaccctg ccctcccctc cggccgacct
      721 gagcggtttc cccggcctgc acctgggccg cgcccggctc taccgcatcc accgggtggc
      781 aaacggacca tggtggttct ccaccgacgg ctccggccgg ttcgacctcc cccgcggcag
      841 cgaccggggt acctgctacc tggcggacga cccgttgggc tgcttcctgg aggtgttccg
      901 gttcacctgg ctcgtcccgg aggaggaggt ccagaggcgg aggctcgcgc gcctcgagct
      961 tcccgaggtc ctcctcgccg actgcacggc gagccgcagc cggcgcttcg ggatcaccgc
     1021 cgagatccac tcgaccacgg actacgggct gacccaacgc tgggccgccg cgttcgccgc
     1081 ggccggcttc gacggggtcc gctatctgct gcgccacgac ccggggcaga tgctatccgg
     1141 ggtcgccctg ttcggccccg ccggcgcgcc ggattgggag gtcccgcccg gtgagcccct
     1201 cggctccgac ctcctcctgg aagccgagcg ccgcttcggc atccgggtgc tgcccacgcc
     1261 gtgaaggtac gccggcgacg ccgggcgtcc tccgccatcc tggtacactg cggccgcgcc
     1321 ggatggcgta tccacccaga gaggagtgca atcgatgaag aagacgaaaa agctgatgct
     1381 caaccgtgag accctgcgga ccatggacgt cgcggggggt gacaagccgg gcacgcaccc
     1441 cgagctgccg aacacgatgg tcacctcctg cgactacacc tactcgtgcc gtgaatggtc
     1501 ctgcacctgc ccggttcaat tgatctatta ggtactttgc cctccggccc cggccggccg
     1561 ccatctcggc gaccgcgcgg cgccggacgc cgaacgccgc atcgtcgccg gttccagcca
     1621 ccggctgtct cctcttcgga gacagcctgg tacgatggag gagatgaagt ctcttgcttc
     1681 ggccctctct ccgttcttct ccgccaagct ccgcaggcga ggagacgagt acttccacct
     1741 ccgcctcgtc cggctcctct cctgcaccgg cgaccaggtg gaggccgtgg tgtccgggcc
     1801 ggaggactac cgggtcgtgc tgcggcgcga agagaagacc gtccgggcga gctgcacctg
     1861 ccccttcgcc agcgatggga ccggggtctg caagcacatc tgggccacgg tcctggccgc
     1921 tgatcaggaa ggcgggctcc gcggccccct cggcggcctg ccgaaccgcg tcgtgtacct
     1981 gagccccgac agggacgaag aggaggggga ggtggacctg tccggcgtcc cctcggcggc
     2041 gaggccgcag agggcttccc cgccgccccg ccttcccgcc tggcgggaga tcctggcccc
     2101 cattgccggc gcgagccccc ttccggcggc ccgccagacg gagatccttt atgtgatcga
     2161 cctgccggga agcctggaac ggcagcggct ctgtctggag atcctgaccc tccagcgcaa
     2221 gcaggacggc tcgcccggag ccctccaccc gctccggatg aaccgcgctc agatcgccca
     2281 gcaccacgag ccgctcgacc gcgcgatctt ccaactgctg ctcggagcgg agacgtcgaa
     2341 cccgtacctg atccgctacc aggaggtccc ccagcacccc tccgtcccta acgagatcgc
     2401 gccggagatc gcccggcgtc tctgtctcac cggccgttgc cgtcttcgtg cccaggatcg
     2461 gcagatcggc gagcagccgc tggcctggta cgacggtccg ccctgggagc tgtggctggt
     2521 ggtccgcgac gagggagacg gcggttgcga ggtcgtcccg gagctgcgcc agggcgatac
     2581 gcggcaggac gcccgtgggc tccctctcct ggatcaggcg ggcctgctgc tcaccggcgg
     2641 cggggtggtc ccggtggaca ccggcggcgc cgcggcgtgg ctgcccttgc tgcggcgcgc
     2701 cggctccttg cgggtgcccg ccgccgagcg cgaggacttc ctggaggtcc tgctggccgc
     2761 gcccgtcctc ccccacctcg agctgccgga ctcgatgcgg gtcgaggagg tgtccgtggc
     2821 tccacagccg cgcctccgcc tgtcgccgcc cgggcatcgc tcatccccgg ccggctggcc
     2881 gacggcgacg gtctcctatc tctatgaaga gctcgaagtg cccgcggggc ccgggcgcag
     2941 ggggatctat cagaaaggac aacgccggtt cctcctccgc gactgggagg cggaggagca
     3001 ggccctcacc cgtttggcgt cattggggtt ccgcaccggg atgcccgatc cacgaggcga
     3061 gccggcgttg cggatcgccc ccagccggat cccgcaggcg gtgcgcgcgc tgctcgccga
     3121 ggggtggtcg gtggaggcgc agggcaagct ctaccggacc gcggagcgct tcgacgtccg
     3181 cgtcacctcc ggcatcgact ggttcgagct ccacggcgac gtcgagctcg gaggcgagac
     3241 cgtcggcctg ccgcgcctcc tcgccgccct gcgccgcggc gacggcttcg tgcccttgag
     3301 cgacggcagc ctcggcctgc tccccgaaga gtggctgaag cgctgggagc ccctggcggc
     3361 gctgggcacg gccgaagggg atcacgtgcg gttcgagatg ccgcaggcgc tcctcctcga
     3421 cgcctggctg gctgccgagc ccgagacgag cttcgacgag accttcacgc gggctcgcga
     3481 gcgcctgcgg agcttctccg gcatcgttcc cgccgccgcg ccggaggggt tccggggcac
     3541 cctccgcccg tatcagcagc tcggcctcgc gtggctccac ttcctgcgcg acttcggcct
     3601 gggcggctgc ctcgccgacg acatgggcct gggcaagacg gtgcaggtgc tggccctgct
     3661 cgagtcccgg cggcagcggc gcgccgccga gggcctgccg cccgcgctgg ttgtggtccc
     3721 ccgttccctg gtgttcaact ggcaggtgga ggccgctcag ttcactcccg cgctgcgggt
     3781 gctcgactat tccggcagcg gccgctcgcg ggacctcgag tcgttcgccg gccacgacgt
     3841 cgtcctcatc acctacggca ccatgcgccg ggacatcggc atcctcaagg aggcgcagtt
     3901 cgactacgtg atcctggacg aggcgcaggc gatcaagaac gccgccagcc agacggccaa
     3961 ggcggcccgg ctgctgcgcg ggcaccatcg cctggccctc accggcaccc cgatcgagaa
     4021 ccatctgggc gagctctgga gcctgctcga gttcctcaac cccggcttct tcggctcggc
     4081 gttccacgcc aagccggccg agctgcgcga ggcggggacc gcggcgcgcg agaccctggc
     4141 gcgcgcgttg cgcccgctga tcctgcgccg caccaaggac caggtggcgc cggagctccc
     4201 tccgcgcacc gagcagacgc tcatctgcga ccttcccaac aagcaacgca agctctatga
     4261 cgagctgcgc gactactacc gcgcggtcct cgaccagcgc atcgcggcgc aggggctggg
     4321 acgctccaag ctcctggtgc tcgaggccct gttgcggctg cgccaggccg cctgtcatcc
     4381 cgcgctgctg gaccgcggcc acgcggacga tccctgtgcc aagctcgacc tgctgctgcc
     4441 gcagctcgag gaggttctcg acgagggaca ccaggccctg gtcttctccc agttcacctc
     4501 cttcctcgcc ctgctgcgcc gccggctcga cgccgccggc ctcccctatc tctatctgga
     4561 tggcaagacc cgcaagcgcc aggagctcgt ggacgccttt cagtcccgca ccgggacccg
     4621 gcttttcctg atcagcctga aggccggcgg gctcggcctc aatctcacgg cggcggacta
     4681 cgtctacctc ctcgatccct ggtggaaccc ggcggtcgaa gcccaagccg tggaccgggc
     4741 acaccgcatc ggccagacgc ggagcgtctt cgcgtaccgg ctggtggccc gtgacaccgt
     4801 ggaggagaag atcctggagc tccaggccgg caagcgcgcc ctcgccgagg ccatcatcca
     4861 ggccgatgac agcctgatcg ccaacctcgg ccgggaggac ctggagctat tgctctcgtg
     4921 aagcggggcg atcgatccgg gcgaagacgt tctcgaccat ccggtcgcag cgggcgccgt
     4981 cgaagaggta gagccggcgc acctcatcgc cgatccgctg gtcgagcctc tcgcgcaggc
     5041 tgcgctttgc ccggtgcagg cgcaccctga cgttctccac cgtgaggccc agcgcgtcgg
     5101 cggtctcctc cgtcgagagg ccctcgatct cgcgcagaca gaagacggac cgcaagggat
     5161 ccgggaggat ctcgaccgct tcccgcagga tcgcatgcaa ctcgcggttc gccagctcct
     5221 tctccggccc gggagtctcc gtgggcggct ccggaggctc atccccgccg atcgaaacca
     5281 gccgccgccg tttccgcgcc cgggccagcg cctcgtggca ggcgatgcgg gtcaaccagg
     5341 tggagaaccg ggcttcgccg cggaaggagg cgagatcacg gaaggcccgc acgtaggcgt
     5401 cctgcgtgac gtcctcggcc tccgcgtcgt ccgtgacgat gccacgggcg acgcggaaca
     5461 gccgctggtt gtagcgccgc atcaggatct cgaaaagcga ggtgtccccc gccagcacgc
     5521 gcgccacgac ctcctcgtcg gagagggccg tgggcgcggc ggcgggtagg agtgctggca
     5581 ggctcatcat ctcggctcca ggtcgattct acgcccggct ctcccgcagg ctggccctgc
     5641 gcgccgccgc gggctcctcc tgccgcagct cggccagccg ggcgagggta tacgcgccga
     5701 ccgccatcgc gaggtcccgg acggcgatgt cgagcaggcc catggtgacg aggttcagcg
     5761 cgatgaggac cagccaggcg gcggcgatcc aggcgccgag ccgggtccag cgcgtgagga
     5821 tcatcacgcc gaccgccatc tcgatgatcc ccaccgcgta catgaacgtc tcgggagaca
     5881 cggggagcag gcccgccatc acggggctca gatactgcgg ccagtcggtc agcaggttga
     5941 agaacttgtc gagccccgcc agcagcggca ccaagccata ggtcagccac aggggccggt
     6001 agagtgattc gaggctcggg ttggaatgag tcgtggtcat cggatcgtcc tcctttactt
     6061 atagagagtc acgaggccgg agaacgttac aaggggacat gcggtagcat ccgccgccat
     6121 ggacatctcc cgtcgccagg ccctggccgg cgccgccgcg ctcgcggccg ccggcgccct
     6181 cccccacccg ctcccggcgc tgaggtcgga cggcagaccc cggaccgtcc ggccgcgccg
     6241 cctccagccc ggcgataccg tgggcctcgt ggacccggcc agcgcggtct gggagccgat
     6301 gaacgtcgag atcgtgaccg agtccctcgc cgcgctcggc ttcaagacga agcgcggggc
     6361 ccacctgctg gcgcggcggg gctacttcgc cggtaccgac gagcagcgcg ccgccgacct
     6421 caacgccatg ttcgcggacc cggaggtgcg ggcgatccag tgcgtccgcg gcggctgggg
     6481 ctcggcccgc ctgctctccc tgctcgactg gaacacgatc gcgcgcagtc ccaagatcct
     6541 cctcggctac agcgacatca ccgcgctcct cctcgccctc cacgcgaaga ccggcctggt
     6601 gaccttccac gggccggtgg gcctctcgca gtggaacccg ttcagcgtcg gttacatcaa
     6661 gcgggtgctc caggaggcgc aagcggtcac gttcgaaaat ctgaaggaga tcgacgagga
     6721 cgacctgacg gtggtggaaa accgggtgca gacgctgcgg cccgggaccg cgcgcggccg
     6781 gctgctcggc ggcaatctca ccgtgctgac gagcctcgtc ggctccgact acctgccgga
     6841 ctgggatggc tgcatcctct tcctggagga tgtagaggaa gcgccctacc ggatcgaccg
     6901 catgctcacc caactccggc tcgccggcat cctgcggaag gcgcgggccg tgatctgggg
     6961 gaactgcacc ggctgccggc cgggagaggg atacggctcg ctgacggtca ccgacgtgct
     7021 ccaggaccac ctcctgcccc tgggcgtccc cgtctggtca ggagccatga tcggtcacat
     7081 cgaccggcag ttcaccttac cggtagggct cgaggtcgag gtggatgcca cggccggcac
     7141 catccgcatg ctggagcccg cggtcaccgg ctgaatctcc gtccccgcgg agacagggct
     7201 catcccgccc ggggccgccg ccgccgatcg agagaaggcc gccatccccg cggagatgat
     7261 cttcataccc cgtgaaacgt tcgtcctacc cggggtacgg acttcatctc ccgggggata
     7321 gccttcgttt tgtggggatg ctggcgagct cccccgggag gagacgtgat attcatgtgg
     7381 agcgcgatca tcaaacggcc aagtactggc tggagccagt cagactagcc cggagcaaag
     7441 ggttcaagag ccaggagctc aatagcattc ggaagatcgt cgaggaaaat cagttcatcc
     7501 tgcggaagga atggaatgac tactttgaga gccgagattc aggtctctag cgcagagcgc
     7561 gtcagcgtca ccgaagagat gttaacggtc gacctgaacg acggaagggt catctccgtg
     7621 cccctgtcct ggtatccgcg cctggtgcag gggactccag aagaacgcgc ccattggaga
     7681 ctcattggcc atggagaggg cattcactgg cccgacctcg atgaggatat cagcgtcgag
     7741 aacctcgtct gcggacaccc ttccggtgag agtcaaagct ctctccagcg gtggcttgag
     7801 aatcgggcca gatagaagtt acgagcctac cccgccggct tcgcctctcc gccctctccg
     7861 tccagctcct cgatgagcgc atcggaggcc gggcgctcct tctggaggcg cgcggcagtc
     7921 ttctcggctt cccgcatgac ccgcaggacg ttcagcccgg cgagcttttg gatgtcctgc
     7981 tcggtccagc tgcggcgcag gagctccgcg aggaggaccg ggaagcggtc caccccctcg
     8041 agccccaggg ggacggcggt gatgccgtcg aagtccgagc cgatcccgac gtggtcgatg
     8101 ccggccacct tgcggacgtg ctcgatgtgg tcggcgatct gggggatggt ggcccggggg
     8161 gcggggttgg ccttcttcca ggcctccagc tcggccttgg cccggtccgg gtccccgggg
     8221 aagagcgcct tgtagcgggc ctcggcggcg tcctcgtcgg ccccccactg ccgcacatcc
     8281 tcggagacga atgaggggac gaaggtcacc atcaccacgc cgccgtcttc cttcaggagc
     8341 tggagcacgt cgtccggcac gtcccgcggg tggtcggtga cggcgcgcgc cgacgaatgc
     8401 gagaagatga ccggcgcctc ggacacggcc atggcgtcct tcatcgagtc gggtgagacg
     8461 tgcgagaggt ccaccagcat gccgaggcgg ttcatctccc gcaccacctc ctcgccgaag
     8521 cgggtgaggc cgccgtgctt cggcccgtcc gtggccgagt cggcccagtc gttgttcttg
     8581 gagtgggtga tggtcatgta gcgggcgccg gcgttgtaga gctggcgcag cgtcccgagg
     8641 gagttgtcga tggagtgccc cccctccatg ccgatcaggg aggcgatctt gcctgccttg
     8701 tggatgcgca cgacgtcgtc cgccgtggtg gcgacctcga agacctcggg atagcgctcc
     8761 gccaggcggt gaaccacgtc gatctgctcc atcacggtct tcaccgccgc cggcccggag
     8821 atgtccgccg ggatgtagac cgaccagaac tgaccgccga cgccgccgcg gcgcaggcgc
     8881 gggatgtcgg tgtgcatggg cttctcgagc ttggaggtgt ccccccggag gtcgatcttg
     8941 tccaggtggt tccggacccg ctggcggtac tcccagggga ggtcgttgtg gccgtcgatc
     9001 aacggcacct tgtcgaggat ctggcgcgcc cgggtgacga gggcggcatc ggggggctgc
     9061 caggccggcg cctgcgccgc agcgggaccg cccgtgaaga cgggccaggt catcatcaat
     9121 ccaagaagcc agctcttctg ccgcatcgtg tctcctcgtt caggtccggc cggagattgt
     9181 agctccgctg cgctagagta cgcgccggag acatccccat ggaccaccct accccattcc
     9241 gccccgaagc ccacgcgctc tacgactccg gcaagatggg caaggccacc ctctatcagt
     9301 cggagcggct gctggtgggc ctgaacgcct tcgagcccgg gcaggagcac aagctgcacg
     9361 cccatgccgg catggacaag gtctaccacg tgctggccgg acagggcctt ttcctgctgg
     9421 agggccggga agagcccatg gagcccggca ccatgctcat cgcgcccgaa ggcgtgcccc
     9481 acggcatccg caacagcggc gccgaacgcc tcctggtgct cgccatcctg gcaccgtcgc
     9541 cctgatcctg ccgcctctcc tcgctgtccc gccccatccc cccttcgtac ctggataagg
     9601 taggccgcgg aggaacggac ctggcggcca aggaggcaac ttggcgaaca tcgccttcgt
     9661 cctcgatcac gaggaggggc atctgctccc tacgttcaag ctggcggcgc ggctcgcgtc
     9721 ccggggccat gccgtgcgct atctgggcct cgcggacgcc gaggacttcg tccggcgcca
     9781 gggtttcgag ttcagcccca tcctggagga cgtctttccc ctgggatcca ccaagacgct
     9841 gcgggagagg acccggggcc aggaccccga ggaggagacc ggcccgatcc acggcgtcct
     9901 cggggccggc gagatctcgg acgagtacct gatctccctc atccacggca gggacctggg
     9961 agacgccgtc gaggacaccc gcccggacct cttcctggtc aacagccact tcgtgctgca
    10021 cgccctggtc ctctcctacc ggttcaagat ccccgtcgtc ctcctcaccc cctggctgcg
    10081 gccgttcccc aagaccggct acacccagat gatcgagacc tccctcctgc agctccggac
    10141 ggccggcctg gagctgttca gcctcgtcca gagcgccgac cccaccgccc gccgcttcca
    10201 ggacatcgcc gcccgcttcc tcgccatgcg ggagctgatc atgtgcccgg ccgagctcga
    10261 gctgccgcgc gacggtcagg cgccgcccga gcacgaggtc taccacgtcg aggcctcggt
    10321 cgacctggcg cgccgcgacg accgggagct cccctgggag cggctcgacc ccaataagcg
    10381 actcctctac tgctccgtcg gcagccagag ccaactggtg ggccgggaga agatcgcccg
    10441 cttcctgggc gcggtggtcg aggcggccac gggcctggcc ggctggcagc tcgtcctcgc
    10501 caccggcggg atggtggggc tccgggacct ccctcccctg ccgccggacg ccatcgcgac
    10561 cggttgggtg ccgcagctcc cggtcctcga gcgggcctcg gcgatgatca cgcactgcgg
    10621 cctcggcacc gtcaaggagt gcatcttcca cggcgtcccc atgctcgccg tcccgatgat
    10681 gcgcgaccag ccggacaacg cgaagcggat cgtccatcac cgtctcgggg tggcctgcga
    10741 cttcgagacc gtcaccgcag gagggatcgc cgccctgatc cggcagctcg acgaggaccc
    10801 cgccatccgc cagagcgtcg cccgcatgca gcgccgcttc cacgagatcg aggactccgg
    10861 cgtcggcgtc cagcgcgtgg aggaggtcct gaacggcaag gcgaggccgg ccgcaggcca
    10921 cgggtaagcg ctctcgcctc gcccgtcctc gcgcttggac cggaccgcca tccggaaggg
    10981 acgctccatc gcgggcgccg ccctggatca ggagagcacg tcccttgcat aagccgtccg
    11041 cagacccgct tctctccaga aagggagtgt ctgatgagac gagcttctgc tttgctgtct
    11101 gtctggatga tcctgtcgat gctcggcgcc ggcctcgcct gggcggctcc cggcacctcc
    11161 accccctcca cgtcccgccc ggccgggaac ccgctcgaga cgctgagcct cgccctggcc
    11221 ggcgcgctcg aagaccgcga tttccgcgat ctcctgcggc agagcctcgc cgcgagcccc
    11281 tcgggcagcg tgtcgctgcg cggcctgctc gccaaggagg ttgcccaggg atcgaccgtc
    11341 gcgcagaggc tggcgcgcgg cctgtcccgc acccatgacg gttctccggg tgaccgcggc
    11401 ggcgcggccg tcaaggcccg cgccctccat cgggtcctcg gcctcctcga ggacatgccc
    11461 cacgtcgacc tgaccgtggc tcccggcctc gcggcttggg acgccgccca ggtcccgctc
    11521 accacctata cccgggacgg ggaggtgacg accgtcgagg cgttcgagcc cgacggctcc
    11581 cgggtcaccc tggacacgcg ccggccgctc ccggtcccgg tgatcgtgct cgagcaccac
    11641 gacaccagcg acgccgtcga gatcgtgagc cgcgcggcgc cggacgccat ggacctcgat
    11701 cacagcgggg tggccagcgc cacggccgcg ctctatgggc agtgctggga ctcgacgacc
    11761 tacaagtcct acccgctgat caccggcgcc tggatctggg atatccacga agggtgctgc
    11821 aacgggcccg aggggtacgc ctatttctcg gtggccggct ccgagaagac cggaaacctc
    11881 ccggccagcc tgggcgggca gagcacctgg ggaaccaacg tctgggcgag caacttccag
    11941 ggcccgaaaa agatcgagaa gacccggctg caatgggagt ggatcgccga cgggtggatg
    12001 gaggggtcga acatggtcta ccccgccgcc tattgccagc agtcggcgac cagcacgtcg
    12061 ggcttctccc aggtggccta caccatcaag gaggacgatg acgggccgtt ctcgtccgac
    12121 gactatgtcg gcaaggtcca gatcgatcac cgccactgca agggcgaggt cttcgacctc
    12181 gggctcgcca gcggctggtc cctcgagcac atgacggccg gcatcgacga cgtggagtac
    12241 acccgctaca agctctactg ctcgacccag acgaactgct cggcgtccgt ggagtgcccg
    12301 aacggcggct ttgtctcctg ctcgggcagc ggcacctgcg gccaggggag ctgcgtggcc
    12361 ggggaggact gggtgcagtg cggcggctac ttcgagagct gctggtccaa tccggagccc
    12421 tgcccgggcg gacagatcgt ctgcgagcaa ccgctgtagg acactccagg gggagaggcc
    12481 ggctacgttc cagaaaaagg acgctccacc cggccttctc cccgcctctt tcctcccgcc
    12541 ccccgccttc agaggtaggc acggacggtg cataagcgcg tcagtgggcg gcgccgccca
    12601 ggcgcagcga gagctaggag gtacttgaca tggcgaagcc gttccacttt ctggcaggag
    12661 ccgcgagcga tggccacggt ttccttcccg cagtcacctg tctcgagccg gtagggacga
    12721 acggggctga cgacaatgtc attcactggt tgctgtatca accgagccag cagttcccgg
    12781 atcagcagtt cgccgtcgcc gtcgccgaga ccgagctctc gggcgccttc accaatccgc
    12841 tcgtcatggc ctccgaccga gccctcgcca cgtatctgca gacacgcggc ttcgtcgagg
    12901 tcgaggttcc cgacgacccg gaggtgaagt gcatcgacac caaggcgctg gcggagagtg
    12961 ccggcgagga gcaggagaag gcctgcgccg acccggcgct gaccaacccc gtggtccagg
    13021 cgctgatcct ccagaccatc gacagccaga tcctcggcaa cgatcccccg gaggcgcggg
    13081 agaccctgca gcgcctggtc ggccagggct tcaccccgga ccaggccaag aacctcatcg
    13141 gcgccgccat cgccaccgag atcttcgagc tgtacgagca caaggcttcg ttcaaccgcg
    13201 aacggtacgt ggagaacctg aagaagctgc ccttcctgcc cgagcgggga gagcagaccg
    13261 aaggctgact ctctgggggc cccgtccggg gcccccagag cttcccatcc cccatcagca
    13321 gtagccgccg cccccctccc ccgcttgtgt acgggcggag cttgtgacat ccttcacgag
    13381 cccggaattc ggagacggaa ccgggacgtt gccgcactgg gaggcacagg ccgtgccggg
    13441 gctggagtcg ccggccacgg cgggagcgtg cttccggaaa ctcagacgat tctggaggaa
    13501 acccgttgcc ggcgcagtac taccctgtcc tgctgacgct cggaatcgcc atcctgatgg
    13561 gcgtggtgat cctcggcctg tcccaggggc tcgcgtggat gatgggcaac cagcgtgggg
    13621 gccgggtgaa gctcaccccc atcgagtccg gcatgcccct gctggaccgc tcccacaagc
    13681 ggctgtccat cgcgttcttc ctggtggcca tcgacttcat cgtcttcgac ctcgaagccg
    13741 cgttcctcta cccctgggcg ctgatcgtgc gcgagggggg atgggagctg ttcgcggcgg
    13801 tgatggtctt catcttcctg atcctggtcg gctacgcgta cgtctggctc aagggcggcc
    13861 tcgagctgcg gccgcagcgc gaacgccaga ccgggagcgc gagaatccag ccatgagcgt
    13921 gagcgaacca gcgactggcg ccgcccccgt ctccggtcat gccggcctgc ctcccgaggc
    13981 caaggccgag ctcgacgaga tccgtcggcg gtacccgacc aacgaggccg ccctgctgcc
    14041 ggccctccac atcgcccaga aggtctgggg cggctggctg cccgacgagg ccatccaggc
    14101 ggtggcccgc gagctcgacc tcgcccccgc caaggtctac ggcgtcgtca ccttctacga
    14161 cctgtaccac cagaagccgg tgggccgcca ccggatccgg gtctgcacca acctgtcgtg
    14221 ccagctccgg ggctccgagg agatcatgga gaccctccac caggagctcg gcgtcggaga
    14281 ggacgaggtc acgccggacg gccgtgtctc ctacgtccac ttcgagtgcc tgggctcctg
    14341 cgacaccgcc ccgatgatga tggtcgacga gacctaccag gagaacctca cccctgaccg
    14401 ggtgcggcag atcctgaaag gattgaagta gatgcctgct tttgaaccgg tcctcacgcg
    14461 gaacgtcggc aagcccgagg cgctgaccct caagggctac cgccagctcg gcggctacga
    14521 ggcgctcgcc aaggccctca agatggagcc cgacgcgatc atcgaggagg tcaagaagtc
    14581 gggcctgcgc ggccgtggcg gcgcgggctt ctccgccggc ctcaagtgga gcttcatccc
    14641 caaggggaag gacaagccca agtacctcgt ctgcaacgcc gacgagtccg agcccggcac
    14701 cttcaaggac cgcctgctga tggagtacga cccgcaccag ctcatcgagg ggtgcgccat
    14761 ctgctgctac gcggtccacg cgaccacctg ctacatctac atccgcggcg agtacaccaa
    14821 ggccgccgcg atcctcgagc gggccttgaa ggaggcctac gagggcggga tcctgggcga
    14881 cagcgtcatg ggctcgggct tcaagctgaa catgtgggtg caccgcggcg cgggcgccta
    14941 catctgcggc gaggagaccg gcatgctcga gtcgctggag ggcaagcgcg gccagcctcg
    15001 ggtcaagccc cccttccccg ccgtcgtcgg cgcgttcggc tcgccgaccg tcatcaacaa
    15061 cgtcgagacg ctctgcaacg tcccgcacat catcgagcgc ggcgccgact ggttcaaggc
    15121 gatcggcacc gacgagcgga acaccggccc caagctctac gccatctccg gccacgtgga
    15181 gcggcccggc acctacgagt acccgatcgg ggtcacgttc cgcgagctgc tcgaggggtg
    15241 cggcggcatg tacaaggggg gcaacctcaa ggccttcatc ccgggcggcg cctcggcgcc
    15301 gatcctgccg gccgagcaca tcgacatcaa gatggatttc gacaccgtcg ccaaggccgg
    15361 ctccatgctc ggctcggcgg cggtgatggt catggacgac aagacctgca tggtggggtc
    15421 ggtcgcccgg ctcgccaagt tcttcaacca cgaatcgtgc ggccagtgca cgccgtgccg
    15481 tgaggggacg aactggatgg agctggtgtt gaagcgcctg gagcacggcg aggggagcga
    15541 ggcggacaag acgctgctcc tgtcggtgtg caaccacatc ggcggcaact ccctgtgcgc
    15601 cctcggcgac gcggccatcg gcccggtgaa gtcgctggtg gagcgctacc ccgacgagat
    15661 cgaccagcac gtgcgcgaga agcgctgccc gttcccgcgg cgccgctact tctcttaacg
    15721 aacggagccc gaagtgccca aagtcacgat caacgaccgc accgtcgagg tggaagccgg
    15781 cacgaccctg gtcgacgccg cgctcaaggt cggtgtccag atcccccact actgttacca
    15841 cccgcgcctc agcgtcgtgg gccagtgccg gatgtgcctg gtcaagatcg aggggatgcc
    15901 caagctccag gccggctgct ccacgacggt ggtcaaggac ggcatggtgg tgcggaccga
    15961 cgtgcccgag gtcgaagagg cgcagaaggg gatgatggag ttcttcctca tcaaccaccc
    16021 tctggactgc ccgatctgcg accaggcggg cgagtgcggc ctccaggact actcgttcaa
    16081 gcacggggtg gcctactcgc gcttccgcta cgaggacaag cgcacctacc ccggccgcga
    16141 gcgcatcccc ctgggcgcca acgtcatcct caacatgaac cggtgcatcc agtgcacccg
    16201 ttgcgtgcgc ttcacccagg agatcgccaa gaccggcgag ctgggcttct tcaaccgcgg
    16261 cgcccgcacc gagatcggca ccttccccgg caaggagctg agcaacccgc tcgccacctg
    16321 cgtggtggac atctgtccgg tcggcgcgct cacctccacc cgcttccgct ttgccgagcg
    16381 cgtctggtac ctggacaagc gcccgtcggt ctgcaccggc tgcgaggtgg gctgcaacat
    16441 caccatggag caccgccggg gtgacatcaa gcgctacaag ccgcgctaca accccgacgt
    16501 caacgactac tggatgtgcg actacggccg cgccaccttc gagcgctaca agcagatccc
    16561 gcgcatgcag gcggtgcggg tcaaggcttc ggccgccggc gtggccggca acggcaacta
    16621 catcgccgcc ggctggaagg aggcgctgga cgcggtgcac cggcagctca ccatggctga
    16681 cggcccggtg gcgttcctcg gctcccccta cctcaccacc gaggaggcgt ggctgttctc
    16741 gcagctcgcc gacgccgtgg gctcgcccca ccgctcggtg ccggtggaca tcgggccgga
    16801 gcgcaccatc cccaacctca aggggggcat caccggccgc gaggcggcgc ccaaccggcg
    16861 cggcgccgag ctggcgggcc tcaccccggg gaccggcggc gacctcgacg cggacgagct
    16921 gatcctcggc gacggcgccg cgcgctgctc ggtgctggtg gtctgcgaca gcgacttctc
    16981 ggccacggcc tacgcgccgg agaccgtcga gaagctccgc cgcgccaaga ccctgatcgt
    17041 cttcggctgg gccgactcgc cgctcgccaa ggtggccgac atcgccctgc cgctcgccca
    17101 ccacgcggag aaggacggca ccttcgtcaa cgtcgagtgg cggctgcaga aggtcgagcg
    17161 cgcgttcccg gcccccggcc aggtccgccc cggcgtggac gtcctggcgg agctcctctc
    17221 ccgcttcgac gcgcactggg ccaaggtcac catcccggcc gccttcgacc gcatggcggc
    17281 ggcgctgccg gcgatggcgg gcctcaactg ggagagcctg ccggcgaccg gcgctcccct
    17341 gaacgtgccc caggcccggc cggcgacatc ggcgaccagc gccgaggcgg ccaaccagga
    17401 ggctggcatc ggatgagcta cgtgccttcg gacctcatcg ccgtcgtcgc caagctggcg
    17461 gtcatgctcg gcttcatcct caccgtcgtg ccggtgatgg tgtgggtcga gcgccgcggc
    17521 agcgccctga tccaggaccg gcccggcccc aaccggctcg gtcccctggg gctgatccag
    17581 ccgctggccg acgccctcaa gttcatgttc aaggaggacg tcacgccgtc ggcggccgac
    17641 aagccgctct atctggcggc gcccgccctc gcgctgctgc cggcgctctc caccatctgc
    17701 gtcatcccct tcgggcccga cctggtggtg aacggcgaga cgcaccgtct ggtcatcgcc
    17761 gacgtgccgg tgggcgtgct gctgttcctg gcgctgtcga gcctcggcgt ctactcgctg
    17821 atcgtggccg gctacgcgtc gaacaacaag ttctcgctgc tgggcgggat ccgcgcctcg
    17881 gcgcagctca tcagctacga gctggcgctg acggtcgcgg tgctggcggt cctcatgccg
    17941 gtgggctcgt tccacctggc ggacgtggtg agctaccagc gcgagcacat ctggaacgtg
    18001 gtgccgcagt tcgtcggctt cgtggtcttc ctgatcgcct cgttcgccga gaccaaccgg
    18061 ctccccttcg acctgcccga agccgagtcg gagctggtgg ccggcttcca caccgagtac
    18121 agcgccatgc gcttcgccag cttcttcatg gcggagtacg cggcgatgac cgcgctgtcg
    18181 gccctggggg tgacgctcta cttcggcggc tggagcctgc cgggggtcga cctcgacggc
    18241 tggctcggcg cgctggtggg cctgatcacc ctggtagtca aggtggccat ctgcatggcc
    18301 gtcttcgtct ggatccgctg gaccttcccg cgcttccgct acgaccagct catgcggctc
    18361 ggctggaagg tcctgctgcc cctggcgctg ctcaacctga tcgtcatcgc cgccttgacc
    18421 gtcgccggct ggctctagag agagggagct ctgatctgat ggagacgctc gtcttcgccg
    18481 tgttcgcctt cctcgcggtc gcttcggcga tcgccgtggt ggcgcaccgc aacccggtct
    18541 actccaccat gagcctggtg gtcacgctct tttcggtggc ggtgctgttc gtgatgctcg
    18601 gggcgccgtt cctgggggtc atccaggtgc tggtctacgc cggcgccatc gtcgtcctct
    18661 tcctgttcgt catcatgctg ctcaacatcc aggccgagga gatgccgcag gggggccaga
    18721 tggcccagcg catcgcctcg atcctgggcg cggtggtctt cgccggcatg ctcggcctgg
    18781 tcttctggcg caccatgcag ccgaactcgc cgccgctcac cgaggagctg gtctcgctgc
    18841 gcaacctgtc gcgggagctc ttcttcgaat acctgctggc gttcgagatg atcggcctgc
    18901 tgctcctggt ggcggtcatc ggcgccactg tggtggcgcg ccggccgcag caggccgaca
    18961 ccaccgaaga ctgggccgcc tcgggcgacg cgccgcgcgt cgacggggcg cggtccgagc
    19021 cccacatcaa cccggagacg ggtgatcgcg tggcggcacg cgcggggagg tcctgatcca
    19081 tggttcccgt ttcctggtat ctgctgctct cggccctgct gttcgccatc ggcaccgccg
    19141 gggccctcac ccgccgcaac ggcatcagcg tcttcctgtc gatcgagctg atgctcaacg
    19201 cggccaacct gtcgctggtg gccttcgccc ggcaattcga cgagcacacc ggacaggtga
    19261 tcgtgttctt cgtcatggcc gtggccgccg ccgaagccgc cgtggggctg gcgctgttca
    19321 tcgccgtcta tcgccagcgg cgcaccatcg acatcaaccg catcaacctg atgcgctggt
    19381 aagggctctc tgacgatgct cgaactgctc tggctcatcc cgctcctgcc gctcgtcggc
    19441 gcggccctga acggcctgct cgcccgggcg cccaagcccg tcaccaccgc catcggcctc
    19501 ggcgcgccgc tggcctcgct ggtgatcgcc ctgggctgcc tgtgggagtt cgtcacccgg
    19561 ctccaccccg ccggccaggc cttccggcag gtgctctacc cctggaccgc gggcgacctc
    19621 aacatcgacg tcgccttccg gctggacgag ctctcggcgg tgatgctgtt cgtggtcacc
    19681 ttcgtgggct tcctgatcca cgtctactcc gtgggctaca tgtcccacga gcacgggtac
    19741 cgccgctatt tctgctacct gaacctgttc atggcggcga tgctgatgct ggtcctgggc
    19801 gacaacttcc tgatgatgtt cgtcggctgg gagggcgtgg gcctctgctc ctacctgctg
    19861 atcggcttct actacgacca ggccttcccg ccgttcgccg gcaagaaggc gttcatcgtc
    19921 aaccgggtgg gcgacttcgc cttcctggtc ggcatgttcg ccctcatcga gcgcttcggc
    19981 acgctgtcat acgacggcat cttcccggtg gtagaggccg acccgggcct ggtgaccggc
    20041 ccctacgtgc tggggctcac cttcgccggc ttcgtcaccc tctgcttctt catcggcgcc
    20101 atgggcaaga gcgcgcagat cccgctctac gtctggctgc cggacgccat ggccggcccg
    20161 acgccggtct cggcgctgat ccacgccgcc accatggtga ccgcgggcgt ctacatggtg
    20221 gtgcggtcga acgtcctgtt ccagctcgcg ccgggggtct cggcgctggt ggccgcggtg
    20281 ggcgcggcga cggccctgtt cgccgccacc atcggcctgg cgcagacgga catcaagaag
    20341 gtgctcgcct actccaccgt gtcgcagctc ggctacatgt tcatggccgc cgggctcggc
    20401 gcctacacgg cggcggtctt ccacctgggt acgcacgcgt tctttaaggc gctgctcttc
    20461 ctcggctccg gctcggtgat ccacgcgatg agcggcgagc aggacatgcg gtcgatgggc
    20521 gggctcaaga agtacctgcc cgtcaccttc tggacgttcg tgatcggcac cgtcgcgatc
    20581 gccggcatcc cgcccttcgc cggcttcttc agcaaggacg agatcctgca cgccgccgcg
    20641 gccgacggca agtggctgct ctgggccgtc gggctgctga ccgccggcat caccgcgttc
    20701 tacatgttcc ggctggtctt catgaccttc ttcggcaagt tccgcggcac gcacgagcag
    20761 cagcaccacc tgcacgagtc gccgccgtcg atgacgatcc ccctgatcgt gctggcgatc
    20821 ggctcggtcc tcgccggctt cgtcaacgtc cccgagctcc tggggggcaa cgaagcgctg
    20881 caccacttcc tggacccggt ctacgcgcag ccgcgggggg ccgaagccgc ccatcacgag
    20941 atgagccacg gggtcgagtg ggcgctcgtc gcgctggcgg tggcggtggc cctggcgggc
    21001 atcttcctcg cctggaccat ctacggcaag aaggggctgg aggggggccg cacctgggcg
    21061 cagcgcttcc ccgccgcgca ccggctgctg gccaacaagt actacgtcga cgagttgtac
    21121 gaccggacca tcgtgcggcc gctcgccgcg ctgtcccggt tcttctggaa ggtggtggac
    21181 accttcgtca tcgacggcgc ggtcaacgcc ggcgccttcc tgacggagct gaccggcgac
    21241 ctcggtcgct tcaccaccac cggcaacgtg cgcaactacg cgctgtactt cttcctgggc
    21301 gtcgtcctgc tcttctggtg gatcgctctc taatatggac acctttctcg aactgctcgt
    21361 cggcaacacg ctgacccggc tgatcttctt cccgacgctg gcctgcctgc ccctgctctt
    21421 cttcccgaag ggcgagggcg ggtaccgcgc ggtgaagatc tacacgctgg ccgtctcgtt
    21481 gatcgagctg gccctgggtc tgctctacgt cgccacccac atgacgtggg gcggcggctt
    21541 ccccctggtg cgggacggcg gtgagtccct ggactggatc cgctcctacg gcatccggta
    21601 cgacatggcg atcgacggca tctcgatgcc gctggtcgtc ctgaccgtga tcctggtgcc
    21661 catcgtcctc ctcggctcgt ggaaggggat cgagcggcac tggcggtcgt tctcggcctc
    21721 gctgctcctc ctgaccaccg gcatgctggg ggcgctcctc gcccatgacc tcttcctctt
    21781 ctacatcttc tgggaggtca tgctggtgcc gatgtacctg ctcatcggcg tctggggcgg
    21841 cgagcggcgg atctacgcgg cggtcaagtt cttcctctac accatggcgg ggagcctgct
    21901 gatgctcatc gccatcctgt ggatggcctg gacctatcag agcctgaacg gcggcgtctg
    21961 gagcttcgcg ctggcggacc tgctgcggct cgagctgccg gcgtcgacgc agatctggct
    22021 gttcgcggcg ttcgccctga ccttcgcgat caaggtgccg atgttcccgt tccacacctg
    22081 gctgccggac gcccacgtcg aggcgccgac gggcggctcg gtgatcctgg cgggcgtgct
    22141 gctcaagctc ggcacctacg gcttcctgcg cttcgccatg ccgctcttcc cgcgggcgac
    22201 cgaggcggcg cagccgctgc tggtcgccct ggcgctgatc ggcatcatct acggcgcgct
    22261 ggtggcctgg gtgcaggtgg acatgaagaa gctggtcgcc tactcctcca tcgcccacct
    22321 cggcttcgtc atgctcggcc tgatggcgat cgacatggtg gcctggcagg gggcgctgct
    22381 gcagatggtg aaccacggcc tgtcgaccgg cgcgctcttc ctcctggtcg gcatgctgta
    22441 cgaccgccgg cacaccaagc agttctccga gttcggcggc ctcgccaagg tgatgccgct
    22501 cttcgcgttc ttcctggtgt tctcgatgct cggctcggtg ggcctgccgg cgctcaacgg
    22561 cttcgtcggc gaattcctga tcctggcggg cagctaccgg accctcggct ggccagcggt
    22621 ggtcgccacc ttcggcgtgg tgctggcggc gatctacctc ctcaagatgg tgcagctcac
    22681 catcttcggt ccgatcacca aggaggagaa ccggggcctc aaggacctct ccggacgcga
    22741 gatcgcggcc ctggtccccc tctgcgtcct gatgctgtgg atcggcgtgg caccccaggc
    22801 cttcctggag cccagccgtg aggccctcgg ccgggtgctc gccgagtacc ggcagagcct
    22861 ggagcggccc gaggtgacgc aggcggcgat ccggccggcg gtggccgtcc aacccacgga
    22921 gcagattgcg gaggcaggtc gatgagtcgc ggtgatctga tgagcctgct gccggagatg
    22981 atcctcaccg gcggcggcgt gctggtcctg atgctggaag cgctggtgcc gcgcatccgg
    23041 cgctccttca ccggcctgtc gctgctggtg accgcggcgg cggcctgggg ggccttcgtc
    23101 gcctggagct ggaagccctc accggaaccc tggctgacct ggaacggcct ggtggagatg
    23161 accggcgtga cgctcgccct ctccctggtg gtgctggtgg ccacggcgct cagccttctg
    23221 gcctcgcagg gctacctgcg ccgggagcgc atcctctcgg gcgagtacca cgccctgctc
    23281 ctctggtgcg cgaccgggct cctgctgatg ctgcggggca ccgagctgct caccatcttc
    23341 gtggcgctgg agctcttctc gctctgcctc tactgcctgg cggcgttcca tcgccaggtg
    23401 aacgtcgcgt cggaagcggc gctcaagtac ttcctgatgg gcgccttcgt cagcgccttc
    23461 atcctctacg gcatcgcgct ggtctacggc gcgacgggca gcacgcggat ggcggagatc
    23521 ggccaggctc tcgaggccgt cggccgcatg ccgctcaccg ccagcctcgg cctgttgctg
    23581 ctggtggcgg gcttcggctt caagctggcc ctggtgccgt tccacgcctg gtcgccggac
    23641 acctaccagg gggcgccgac gccgttcgcc gccttcctct cggtggcgcc caaggtggcg
    23701 agcgcgctgg tcctgttccg cgtgctcgag gccgcggcgc agggtggcgc ggccgccacg
    23761 tccggccggt ggtcggggct ggtggcgatc ctcgccgtgc tctccatgct ggtggggaac
    23821 ttcctggccc tggtgcagcg tgacatcaag cgcatgctcg cctactccgg tatcgcccac
    23881 atgggctacc tgatgctggc gatggtggtg ctcgacgacg cgtcgctgcc tccagtcgtg
    23941 gtctacctgg cggcctacgt gctgatgaac gccggcgcct tcgccatcgt caccatgctc
    24001 tacagccgtg ccggcgagca gcacctgatc tcggacctct cgggatgggg ctaccgctac
    24061 ccgctgctcg gcgcctgcct gaccatctgc atgctgtcgc tggcgggcat cccgccgacc
    24121 gcgggattcc tcggcaagta cctggtgttc ctgaacgccg tgggtgacgg ccgggtgggg
    24181 ctggcgatcg tgggcgtgct gagcagcctg gtgggcgtct tctactacct gcgggtgatc
    24241 tacgtcctct acatgaaggc ggagagccgc cagccggagg gcctgctcat cgacggctgg
    24301 ggccgcgccg cggccgtgct ctcggctctc ggcgtgctgg tgctggggat ctggccgacc
    24361 ggtatcctcc agtggttatg ggaggcgacc ggggcccggt gagtgagctg gaggaggtct
    24421 ctccactcac tctcaaccgg ctctccttct acctccgctg cctccggcac ctacaggagc
    24481 tggggatcaa gcgcatctcc tcgcaggaga tggcgcgccg gtaccatctc tcggcgacgc
    24541 agatccgcaa ggatctggcg cagttcggcg agttcgggat ccgcggcgtc ggctacgagg
    24601 tcgacgagct ggcggaccac ctgaactcgc tgctgggcct cgaccgcaag cacgcgctcc
    24661 tcatcgtcgg catgggcaac ctggggagcg cgctcgcctg ctacctgggc ttcaactacg
    24721 gtgccttcca cgtggtggcg gcggtggaca acgacccgac caaggtgggc cgccggttcg
    24781 gcgagctcgc cgtccgccac gcgtcggaga tggccgaggt ggtgcgcgag tccggagcgg
    24841 agatcggcgt gctcaccgtc ccgcccgacg ccgcgcagga gaactacgac gccctggccg
    24901 acgccggcat ccggggggtc ctcaacttcg ccccggtccg cgtcaagcgc cgcgccgggg
    24961 tcccgctgaa gaacgtcgac ctgcgcatca acctcgagga gctagccttc ttcctccgcg
    25021 ggtgaggcca cggacacccc cgcctcccct ccccgctcgg acgaccgcct cgacgccctc
    25081 ctctggttcg tcgtcccggc gctgatcctc ttcctgctgc ccgtggggca cgtctccaac
    25141 gacggcctcg ggcacagctt cgcctttgcc gaggggagct ggcggatcaa ccccaaccac
    25201 ctcctcttcg agccgctggg cgcctggtgg caggggctct ggacgcggat cgacccggac
    25261 cgggcgccgg tggacgcgct caagctcctc tcgggactct ccggagcgct ggcggcgggg
    25321 ctgttccggt ggggtgtggc cggccggctg gcagagtcac gttgggccgc gaaccatgcc
    25381 accgcctggc tggccttctc ctccgccttc ctgcggctgt ggatctcgga cgagatccac
    25441 atgatccaga tgcccttcgt ggtggccgcc gcggtgatgg ctctccggta tctggagcgg
    25501 ccggcgctat ccagggcgct ggcgacgggc gccgccgtgg gcctcgccgg gctcggcttc
    25561 atctccaacc tgctgctggg accggcgctc gctctcggcc tcgcctggtg gcacctgcgc
    25621 cggcgggacg cccggtccgc cgtgacagcc atcgcgggga tcggcgtcgg ctcggcggtg
    25681 gtggccggct cggccttcct ggcagtctgg gcctcctccc ccgctcccgt gggcttcgtg
    25741 gattggctca cccggtacgg cggcggggcc ccgggggagc gggtcg
//
LOCUS       DASPJI010000063         5664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_292774, whole genome shotgun sequence.
ACCESSION   DASPJI010000063 DASPJI010000000
VERSION     DASPJI010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292774"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VEL74_01705"
     CDS             complement(<1..365)
                     /locus_tag="VEL74_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61270.1"
                     /translation="MERMEPDRRPTPTMESRPPFWAKALATLTGNLFLVLGSALLGTI
                     SLLVSWIPPRGSWVFGVSRLWARLLLTASWVRVEARYDPALDPGASYVFLANHQSMFD
                     IPALFVTVPGQIRMMAKKSL"
     gene            442..849
                     /locus_tag="VEL74_01710"
     CDS             442..849
                     /locus_tag="VEL74_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61271.1"
                     /translation="MTATPRFARRFLFLRLLVGSLVLGAFYDLAYAVVMVAAPQVPAR
                     AFNLPLPPPEGTFYLWILAVVLAMLAALYLLAARDPRRYSGIIAVAMGGRILGGLVLL
                     AAALRGLPGLYPMAVADLVFGLAHAAFWLPIRS"
     gene            846..1598
                     /locus_tag="VEL74_01715"
     CDS             846..1598
                     /locus_tag="VEL74_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG61272.1"
                     /translation="MKTRSFIAEAGRLAFLTAQAFAALLKPPYEIRSWVRQMEQIGVR
                     SLGVASITTIFTGMVLALQTALSLPSLGVKYYIGSVVSKSLVRELGPVLTALIVGGRI
                     GSGMTAEIGTMKVTEQIDALRSMAADPVKKLVVPKLAATLIMLPALTLIGDALGILGG
                     LIVATFTLDLPPGLYINDVFDSLTFDDVGSGVAKSFFFAYFIAIVGCYNGLNTTGGAD
                     GVGRATTNTVVVASILVLVSDFFLTKLFYLLA"
     gene            1621..2379
                     /locus_tag="VEL74_01720"
     CDS             1621..2379
                     /locus_tag="VEL74_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG61273.1"
                     /translation="MPEPLFQIRGLVKSYDGRTVLDGVDLDIHRGECLVILGRSGSGK
                     SVTLRQLNGLEKPDAGSVAFDGINVSELDERELFPVRRRIAMLFQSGALFDSMNVRDN
                     IAFPLREHTDLGEAEIAGRVRERLAMVHLKEVEEKMPSELSGGMRKRAALARSLALDP
                     EVVLFDEPTTGLDPTTSATIGKLIDSIQGTLGVTAVVVTHDIPLARRVGDRLAYLSNG
                     KFTFVGPWAEADRADNREFADFLAGREEEDEDAA"
     gene            2369..3787
                     /locus_tag="VEL74_01725"
     CDS             2369..3787
                     /locus_tag="VEL74_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HYG61274.1"
                     /translation="MPPKSSEFKVGLVLLVALAVAAAGIFLIGEKNNLFSRKNEYFIE
                     FDSVSGLKPGSPVQLDGVEVGTVSRVVLPEDPTRANIQVWIKIDNQYAERVRGPQGPG
                     TGRRPAANAPVWTVARIKTLGLLGDKFIEITSGSPEYPPIPEDGRISAADPTNVDALI
                     ASGEDVMDNVVEISASLSTILGRMERGEGLLGELTSDSPSGQRLKESLIGTSETLERI
                     AAKVETGDGPLPRLLNDRAMAEQLAGSLDRFEALLITAQTGEGLVPGLLNDPGMRLQT
                     QETLATLNQVARDLRTFTADLETSDALVPRLVNDEEYGREITGELRQIVDRLSTVSER
                     LVDGEGTVPKLINDPQIYEAVNDVIVGVNESRLLRWLIRNRQKAGIKKRYEDTREAME
                     QAGQTPPPLPEGSEAEDTEPAPPGPLGAVAEKLVEPPAEPAPEATDAPDATPTPTPPA
                     AGTPPAADTPPPPPAAAPPHGS"
     gene            3797..4765
                     /locus_tag="VEL74_01730"
     CDS             3797..4765
                     /locus_tag="VEL74_01730"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HYG61275.1"
                     /translation="MASHILVTGGAGFIGSHLTRRLLARGDRVTVLDDFNDFYDPARK
                     RDNVAPLLGRPDYRLAEGDIRDAALVDRLFAEGRFDAVVHLAARAGVRPSLQEPVLYE
                     DVNCIGTLRLLEAARRHGPKIFLFGSSSSVYGINEKVPFSEADEVNQPISPYATTKRA
                     GELLCYNYHHLYGFRTACLRFFTVYGPAQRPEMAIHKFTDLLANGKTVPMYGDGESRR
                     DYTYVDDIVDGLVATLDLAPGFEILNLGGAQTTSLKDLVHWIAAELAVEPRIDYLPAQ
                     PGDVPITYADVSKAERLLGYSPKVPIREGLRRFVAWYRERHAAVSR"
     gene            4827..>5664
                     /locus_tag="VEL74_01735"
     CDS             4827..>5664
                     /locus_tag="VEL74_01735"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013452108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HYG61276.1"
                     /translation="MNICVVGSGYVGLVTGACLADFGMNVVGVDKDASKVEALSRGKV
                     PIYEPGLETLVQKNMEEGRLRFSTDLGPAIEEAQAVFIAVGTPPKSDGSADLTFIREV
                     ATSIAQHLNGYKIIVTKSTVPIGTGKMIEALVREGAGGKHRFAVVSNPEFLREGSAIH
                     DFLNPDRIVIGADDQAAAVRVAALYLGIAAPLIVTDPPSAETIKYAANAFLATKISFI
                     NAVAAVCEAVGADIKDVALGMGYDSRIGHEFLRPGPGWGGSCFVGGETLFVRRLGGAP
                     RLM"
BASE COUNT          878 a         2071 c         1849 g          866 t
ORIGIN      
        1 aggctcttct tggccatcat ccggatctgc cccggcaccg tgacgaacag cgccgggatg
       61 tcgaacatgc tctggtggtt ggcgaggaag acgtagctcg cccccgggtc gagcgccgga
      121 tcgtagcgcg cctccacccg cacccagctc gcggtgagca gcaggcgcgc ccagaggcgg
      181 ctgacgccga acacccagct cccccgtggc gggatccagc tcaccagcag cgagatcgtc
      241 cccaggaggg cgctgcccag caccaggaac aggttgccgg tgagcgtggc gagggccttg
      301 gcccagaagg gggggcggga ttccatggtc ggggttggac gccggtccgg ctccattctc
      361 tccaccgcca ccgttcctgg caaggcccat gcaaggcccc gggcaagacc agatccggct
      421 ccttgataga atccccccga tatgacggca acgccccgct tcgcccgccg cttcctcttc
      481 ctccggctgc tcgtcggcag cctggtgctg ggcgccttct acgacctcgc ctacgcggtg
      541 gtgatggtgg ccgcgccaca ggtgccggcc agggcgttca acctgccgct gccgccgccg
      601 gaggggacgt tctacctctg gatcctcgcc gtcgtcctgg cgatgctcgc cgccctctac
      661 ctgttggccg cccgcgaccc gcggcgctac agcggcatca tcgcggtggc catggggggg
      721 cggatcctcg gcggcctggt gctgctcgcc gccgccctgc gcggcctccc cgggctctac
      781 cccatggcgg tggcggacct cgtcttcggc cttgcccacg ccgcgttctg gctgcccatc
      841 cgctcgtgaa gacccgcagc ttcatcgccg aggccgggcg gctcgcgttc ctcaccgccc
      901 aggcgttcgc cgccctgctc aagcccccct atgagatccg ctcgtgggtg cggcagatgg
      961 agcagatcgg cgtccgctcc ctgggcgtgg cgagcatcac caccatcttc accggcatgg
     1021 tgctcgccct gcagaccgcc ctctccctgc cgtcgctggg ggtcaagtac tacatcggct
     1081 cggtggtgtc gaagtcgctg gtgcgggagc tcgggcccgt cctcaccgcc ctgatcgtcg
     1141 gcggccgcat cggctccggc atgaccgccg agatcggcac catgaaggtg accgagcaga
     1201 tcgacgccct gcgctccatg gccgccgacc cggtcaagaa gctggtggtg cccaagctcg
     1261 ccgccaccct gatcatgctg cccgccctca ccctgatcgg cgacgcgctc ggcatcctcg
     1321 gcggcctgat cgtcgccacc ttcaccctcg acctgccgcc gggcctctat atcaacgacg
     1381 tcttcgactc gctgaccttc gacgacgtcg gcagcggtgt cgccaagtcc tttttcttcg
     1441 cgtactttat cgccatcgtc gggtgctaca atggcctcaa caccacgggt ggcgccgacg
     1501 gcgtgggccg ggcgacgacc aatacggtgg tggtcgcctc gatcctcgtt ctggtctccg
     1561 acttcttcct caccaaactg ttctacctgc tggcctgaat gagaacgaga aggagaaccc
     1621 ttgcctgaac cgctcttcca gatccgcggg ctggtcaagt cgtacgacgg caggaccgtg
     1681 ctcgacggcg tcgatttgga catccaccgt ggcgagtgcc tggtcatcct gggccggtcg
     1741 gggagcggca agagcgtgac gctgcggcag ttgaacggcc tcgagaagcc cgacgccggc
     1801 agcgtcgcct tcgacggcat caacgtctcc gagctcgacg agcgcgagct cttccccgtc
     1861 cgccgccgca tcgccatgct gttccagagc ggcgccctgt tcgactcgat gaacgtgcgc
     1921 gacaacatcg ccttcccgct gcgcgagcac accgacctcg gcgaggccga gatcgccggc
     1981 cgggtgcgcg agcggctcgc catggtccat ctgaaggagg tcgaggagaa gatgccttcg
     2041 gagctgtcgg gcggcatgcg caagcgcgcc gccctcgccc gttcgctcgc cctcgacccc
     2101 gaggtggtgc tgttcgacga gccgaccacc gggctcgacc ccaccacctc ggcgaccatc
     2161 ggcaagctga tcgattccat ccaggggacc ctgggggtca ccgcggtggt ggtcacccac
     2221 gacatccccc tggcccgccg ggtgggcgac cgcctggcct acctctccaa cgggaagttc
     2281 accttcgtcg gcccctgggc cgaggcggac cgcgcggaca accgggagtt cgccgacttc
     2341 ctggccggcc gcgaagagga ggacgaggat gccgcctaag agcagcgagt tcaaggtcgg
     2401 cctggtgctc ctcgtcgccc tggcggtggc cgccgccggc atcttcctga tcggcgagaa
     2461 gaacaacctc ttctcgcgca agaacgagta cttcatcgag ttcgactcgg tgagcggctt
     2521 gaagcccggc agcccggtgc agctcgacgg cgtcgaggtg gggacggtgt cgcgcgtcgt
     2581 gctgcccgag gacccgacgc gggcgaacat ccaggtctgg atcaagatcg acaaccagta
     2641 cgccgagcgc gtccgcgggc cgcagggccc gggcaccggc cgccgtcccg ccgccaacgc
     2701 cccggtgtgg acggtggcgc gcatcaagac cctcggtctc ctgggcgaca agttcatcga
     2761 gatcacctcc ggcagcccgg aatacccgcc gatccccgaa gacggccgga tctccgccgc
     2821 cgaccccacc aacgtcgacg ccctcatcgc ctcgggcgag gacgtcatgg acaacgtggt
     2881 ggagatctcc gcctcgctct cgaccatcct cggccgcatg gagcgcggcg aggggctgct
     2941 cggcgagctg acctcggact ccccctccgg ccagcggctt aaggagtcgc tgatcggcac
     3001 ctccgagacg ctggagcgga tcgccgccaa ggtcgagacg ggtgacggcc ccctgccgcg
     3061 cctgctcaac gaccgcgcca tggccgagca gctcgccggc tcgctcgacc gtttcgaggc
     3121 gctgctgatc accgcccaga ccggcgaggg gctggtcccc ggcctgctca acgatcccgg
     3181 gatgcgcctg cagacgcagg agaccctggc gacgctcaac caggtggcgc gggatttgcg
     3241 tacattcaca gccgatctgg agacgagcga tgccctggtt ccccgtctgg tcaacgacga
     3301 ggaatacggc cgcgagatca ccggcgagct gcgccagatc gtcgaccggc tgagcaccgt
     3361 ctccgagcgg ctggtcgacg gcgaggggac ggtcccgaag ctgatcaacg acccgcagat
     3421 ctacgaagcg gtcaacgacg tcatcgtcgg cgtcaacgag tcccggctgc tgcgctggtt
     3481 gatccgcaac cggcagaagg cggggatcaa gaagaggtac gaggacacgc gcgaggccat
     3541 ggagcaggcc gggcagaccc cgccgccgct ccccgaaggc agcgaagccg aagacaccga
     3601 accggcaccg cccggccctc tgggagctgt ggcggagaag ctggtcgagc cgccggccga
     3661 gcccgcgccc gaagcgaccg atgcacccga tgccacaccg acgccgacac cccccgcggc
     3721 cgggacaccc cccgccgccg acacgccgcc gccgcctccg gcggcggcgc ctccgcacgg
     3781 gagctgatct ccgagcatgg cttctcacat cctggtgacc ggcggcgcgg gcttcatcgg
     3841 ctcgcatctg acgcggcgcc tgctcgcccg gggcgaccgg gtgacggtgc tcgacgattt
     3901 caacgatttc tacgacccgg cgcgcaagcg cgacaacgtc gccccgctgc tcggccgccc
     3961 ggactaccgg ctggccgagg gggacatccg cgacgccgcc ctggtcgacc ggctgttcgc
     4021 cgagggccgc ttcgacgccg tggtccacct ggcggcgagg gccggcgtgc ggccgagcct
     4081 gcaggagccg gtcctctacg aggacgtcaa ctgcatcggc accctgcgcc tcctggaggc
     4141 ggcgcggcgg cacgggccga agatcttcct gttcggctcc tcctcctcgg tctacggcat
     4201 caacgaaaag gtgccgttct ccgaagcgga cgaggtcaac cagccgatca gcccgtacgc
     4261 caccaccaag cgggcgggcg agctgctctg ctacaactac catcacctgt acggcttccg
     4321 gaccgcctgc ctgcgcttct tcaccgtgta cggaccggcg cagcggcccg agatggcgat
     4381 ccacaagttc accgacctgc tggccaacgg caagacggtg ccgatgtacg gcgacggcga
     4441 gagccgccgc gactacactt atgtggacga tatcgtcgac gggctggtgg cgaccctgga
     4501 cctggcgccg gggttcgaga tcctcaacct gggcggggcg cagacgacct cgctcaagga
     4561 cctggtgcac tggatcgccg cggagcttgc cgtcgagccc cgcatcgact atcttccggc
     4621 gcagcccggg gacgtcccca tcacgtacgc cgacgtctcg aaagccgagc ggctcctggg
     4681 atactcgccc aaggtcccga tccgcgaagg cctgcgccgt ttcgtcgcct ggtatcgcga
     4741 gcgtcacgcg gcggtcagcc gatagcggca gccgcgcgga gccacgctga aacttccaac
     4801 gtcccaattg cgagagctca cgagccatga acatctgcgt cgtcggtagc ggttacgtcg
     4861 gtctggtgac gggtgcctgt ctggcggact tcggtatgaa cgtcgtcggc gtggacaagg
     4921 acgcctccaa ggtggaagcc ctctcccgcg gcaaggtgcc catctacgag ccgggtctcg
     4981 agaccctggt gcagaagaac atggaggagg ggcggctgcg cttctccacc gacctggggc
     5041 cggcgatcga ggaggcccag gcggtcttca tcgccgtcgg cacgccgccc aagtcggacg
     5101 gctcggccga cctcaccttc atccgcgagg tggccacctc catcgcccag cacctgaacg
     5161 gctacaagat catcgtcacc aagagcacgg taccgatcgg caccggcaag atgatcgagg
     5221 ccctggtgcg cgagggggcg ggcggcaagc accgcttcgc cgtggtctcc aaccccgagt
     5281 tcctgcgcga gggctcggcg atccacgact tcctcaaccc cgaccgcatc gtgatcggcg
     5341 ccgacgacca ggcggccgcc gtgcgcgtcg ccgcgctcta cctcggcatc gctgcgccgc
     5401 tgatcgtcac cgacccgccc tcggcggaga ccatcaagta cgccgccaac gcgttcctcg
     5461 ccacgaagat ctcgttcatc aacgccgtcg ccgccgtctg cgaggcggtc ggcgccgaca
     5521 tcaaggacgt cgccctcggc atgggctacg acagccgcat cggccacgag ttcctccgtc
     5581 ccggcccggg ttggggcggg agctgcttcg tcggcgggga gaccctgttc gtccgccgtc
     5641 tcggcggtgc accgcggctg atgc
//
LOCUS       DASPJI010000064         3509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_633146, whole genome shotgun sequence.
ACCESSION   DASPJI010000064 DASPJI010000000
VERSION     DASPJI010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633146"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1146)
                     /locus_tag="VEL74_01740"
     CDS             complement(115..1146)
                     /locus_tag="VEL74_01740"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HYG61277.1"
                     /translation="MDNGTPSGLLSRSPEEGARLLALAYLDQAAAARLRLEDPSDTEA
                     LHDFRVGLRRLRSCLRSYRRYLEGSIPGKLAKRLRRLASSTGPGRDTEVQIEWLRGRS
                     RHLSSQHRTGLAWLLARLEERMREAYEDLGDRIPQEFPELEEKLRHRLSIYRAEIHLG
                     ALSHRVTFGEATAEILRDQIAELTGHLARVDDAEDQEEAHEARISAKRLRYLIEPLVA
                     DLPAAAPLVKRFKALQDLLGDLHDAHVLEIELGEAVEEAAAERARKLLEITLSDAPDE
                     TLLRAERRRSREAGLLTLARLNRARRNRLFETLDAEWLEGRAGPFLEEVEALGRVMGR
                     DEESGGVCA"
     gene            complement(1159..1662)
                     /locus_tag="VEL74_01745"
     CDS             complement(1159..1662)
                     /locus_tag="VEL74_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009626558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HYG61278.1"
                     /translation="MRLLIIRHAIAEEREDFALTGKDDSQRPLTDEGRRKMKQGAKGL
                     RELVPEIDLLATSPLTRAAQTAAIIDSVYDGLDEVEIHELSPEATPEEFLRWLRQQKG
                     GTLAVVGHEPSLSLLLSWLLTGTEKRLFSFRKGGACLLEFSGEVGPGMATLLWALAPA
                     QLQRMAD"
     gene            complement(1853..3418)
                     /locus_tag="VEL74_01750"
     CDS             complement(1853..3418)
                     /locus_tag="VEL74_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor I family protein"
                     /protein_id="HYG61279.1"
                     /translation="MKIGHSGLAGLLALTAWTSVEAAAPGSIVLTPIGTFRTASAEIG
                     SYDPDSRLLFTVGGAANQVNVLRLDRPWQIRPALTIDLAPYGGGVNSVATRDGIIAVA
                     VEAVNRQAPGKVVFFDGAGRALSSVTVGAVPDMLTFTPDGSYLLVANEGEPLDYCAAG
                     LANDPEGSVSVIDLRGGVGLLTQAAVRTAGFAAWNAGAPAGVRIFGPNASVAQDLEPE
                     YIAVSPDSRTAWVTLQENNALAVVDIASATVTSLAALGTQDHSQPRNALDASDRDDAV
                     RIVPRPVQGLYMPDTVAAYRAGGQDYLVLANEGDARDYECFGEETRVGDLDLDAAAFP
                     AAATLQADEELGRLRTTTATGDMDDDGDNDAIFTFGSRSFSIRTAAGALVWDSGSDFE
                     LITAAALPGEFNSTNDENGSFDSRSDDKGPEPEGLTVGNFRGREYAFISLERVGGIMV
                     YDVTDPRAPFFVQYVNGRDFGGDAEAGTAGDLAPEGVLYIPREQSPINRALLVVTHEV
                     SGTVTVYSIKPRS"
BASE COUNT          484 a         1173 c         1321 g          531 t
ORIGIN      
        1 ggagggattc ccactgcgtt ttaaggatcg ccaaaggttc catggcacga gattatagga
       61 cctcgtgcca tgaagacgct aagttagcgc ctatggggcc ggggggtgag gactctatgc
      121 gcagacgccc ccgctctcct catccctccc catcacccgc cccagcgcct cgacctcttc
      181 caggaacggc ccggcgcgcc cttcgagcca ttcggcgtcc agggtctcga acaggcggtt
      241 gcggcgggcg cggttcaggc gggccagggt cagcagcccg gcctcgcggg agcgccggcg
      301 ctcggcgcgg aggagggttt catccggcgc gtcggagagg gtgatctcca ggagcttgcg
      361 cgcccgctcg gcggcggcct cctcgaccgc ctcgccgagc tcgatctcga gcacgtgggc
      421 gtcgtgcaga tccccgagga ggtcctggag cgccttgaag cgcttgacca gaggggcagc
      481 ggcggggagg tccgccacca gcggctcgat gaggtagcgc aggcgcttgg cgctgatccg
      541 cgcctcgtgc gcctcctcct ggtcctcggc gtcgtcgacg cgggctaggt gaccggtcag
      601 ctcggcgatc tggtcgcgca ggatctccgc cgtcgcctcg ccgaaggtca cccgatggct
      661 cagcgcgccg aggtggatct ccgcccggta gatggagagc cggtggcgta gcttctcctc
      721 gagctcgggg aactcctggg ggatgcggtc gccgagatcc tcgtaggcct cgcgcatcct
      781 ctcttccagg cgcgccagca gccaggcgag gcccgtgcgg tgctgggagg agaggtgccg
      841 cgagcggccg cgcagccatt cgatctggac ctcggtgtcg cgacccgggc cggtggagct
      901 ggcgaggcgc cgcagccgct tggccagctt gccggggatg ctcccctcca ggtagcggcg
      961 ataggagcgg aggcagctcc gcaggcgccg gaggccgacc cggaaatcgt gcagggcctc
     1021 ggtgtccgag gggtcctcca gccggaggcg ggccgccgcc gcctggtcga gataggcgag
     1081 ggcgaggagg cgggccccct cctcggggga gcgggagagg aggccggagg gggtgccgtt
     1141 gtccatgggg agcgcgtcct agtccgccat cctctggagc tgcgcgggcg ccagcgccca
     1201 gagcagcgtc gccatgccgg gcccgacctc gccggaaaac tccagcaggc aggccccccc
     1261 cttgcggaag gagaagagcc gtttctccgt gccggtgagc agccacgaga ggagcaggct
     1321 gaggctcggc tcgtggccca ccacggccag ggtcccgccc ttctgctgcc gcagccagcg
     1381 caggaactcc tcgggggtgg cctcggggga gagctcatgg atctccacct cgtcgaggcc
     1441 gtcgtagacc gaatcgatga tcgcggcggt ctgcgcggcc cgggtcagcg ggctggtggc
     1501 caggaggtcg atctccggca ccagctcgcg cagccccttg gcgccctgct tcatcttcct
     1561 gcggccctcg tcggtgagcg gccgctggct gtcgtccttg ccggtgagcg cgaagtcctc
     1621 gcgctcctcg gcgatggcgt ggcggatgat cagaagtcgc atgcagtcct ccccgtctct
     1681 ccatctctat agatataccg cttcgcgcga ttccaccaag aggcttcccg ggagccacaa
     1741 gcaaaaaaag ggccgggtga gacggacggc agagccgcac cgcctccccc ggcgagacga
     1801 caggcgcgga ggttcaggtc tcgcccgccg tctgaagcgt gtcgccgcga gatcagctcc
     1861 tcggcttgat cgagtagacg gtcaccgtgc cgctcacctc gtgggtcacc accagcaggg
     1921 cccggttgat cgggctctgc tcgcgcggga tgtagagcac cccttcgggc gccaggtcgc
     1981 cggcggtccc ggcctcggcg tcgccgccga agtcgcggcc gttgacgtac tggacgaaga
     2041 agggggcccg cgggtcggtg acgtcgtaga ccatgatgcc gcccacccgc tccaggctga
     2101 tgaaggcgta ctcccggccc cggaagttgc cgacggtcaa cccttcgggc tcgggtccct
     2161 tgtcgtcgct gcggctgtcg aagctgccgt tctcgtcgtt ggtggagttg aactccccgg
     2221 ggagggcggc ggcggtgatc agctcgaagt cgctgccgct gtcccagacc agggcgcccg
     2281 ccgccgtgcg gatggagaag gagcgtgagc cgaaggtgaa gatggcgtcg ttgtcaccgt
     2341 catcgtccat gtcgccggta gcggtggtgg tgcggagccg gcccagctcc tcgtcggcct
     2401 ggagggtggc ggcggcgggg aaggcggcgg cgtcgaggtc gaggtctccc acccgggtct
     2461 cctcgccgaa gcactcgtag tcgcgggcgt ccccctcgtt cgccaggacc aggtagtcct
     2521 gcccgccggc ccggtaggcc gccacggtgt cgggcatgta gagcccctgg acgggccgcg
     2581 gcacgatgcg gaccgcgtcg tcgcggtcgc tggcgtcgag cgcgttgcgc ggctgcgagt
     2641 ggtcctgggt gcccagggcg gcgagcgagg tgacggtggc ggaggcgatg tcgaccaccg
     2701 ccagggcgtt gttctcctgc agcgtcaccc aggccgtgcg ggagtcgggg gagaccgcga
     2761 tgtactcggg ctccaggtcc tgcgccaccg aggcgttggg gccgaagatc cggacaccgg
     2821 cgggggcgcc cgcgttccag gcggcgaagc cggcggtgcg caccgcggcc tgggtgagca
     2881 ggccgacgcc gccgcgcagg tcgatgacgc tcaccgagcc ctcgggatcg ttggcgagcc
     2941 cggcggcgca gtagtccagg ggctccccct cgttggccac cagcaggtag gagccgtcgg
     3001 gggtgaaggt cagcatgtcg ggcacggcgc cgacggtgac cgacgacagc gcgcggccgg
     3061 cgccgtcgaa gaagaccacc ttgcccggcg cctggcggtt cacggcctcc acggctacgg
     3121 cgatgatgcc gtcgcgggtg gcgacgctgt tgacgccgcc gccgtagggg gcgaggtcga
     3181 tggtcagggc gggcctgatc tgccagggtc ggtcgagccg cagcacgttg acctggttgg
     3241 cggcgccgcc gacggtgaac aggagccgcg agtcggggtc gtaggacccg atctccgccg
     3301 aagcggtccg gaaggtgccg atgggggtga gcacgatgct ccccggggcc gccgcttcca
     3361 cggaggtcca tgccgtcagg gccaggaggc ccgcgagccc gctgtgtccg atcttcatcc
     3421 gttcataccc tcctcattgg gcgttgtcag agcagaaccg gctggcgccg gatggaggca
     3481 acgctatgag gacgggtcgg aggatgccg
//
LOCUS       DASPJI010000065         5445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_62798, whole genome shotgun sequence.
ACCESSION   DASPJI010000065 DASPJI010000000
VERSION     DASPJI010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62798"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>5445
                     /locus_tag="VEL74_01755"
     CDS             <1..>5445
                     /locus_tag="VEL74_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61280.1"
                     /translation="LERGPLFRPYLLRLSAEDHVVLASMHHIVSDGWSLSILVRELVA
                     LYEAFRAGQASPLPELPVQYADYAVWQRRWLAGDVLEAELAWWRQRLAGLPALLELPT
                     DRPRPPVQTYAGDGRPVLIGRETSEALAGLALASGATLFMALLAVFQGLLGRYAGQTG
                     LAVGSPMAGRGRSELEPLIGFFVNTLVLPAELAGRPGFRELLERTRRRTLEAYAHQEV
                     PFERLVEELAPERSLAYPPLFQVMFVLQNTPREDLRLPGLRLTSMGWGETRTAKFDLT
                     LSLTETGEGLAGTLEYNTDLFDGTTIDRMAVHLERLIAAALAEPETPLAALSFLSAPE
                     SHQLTAEWNDTAISTPAGLPVHLLFESQAARTPDAPAVSFAGEVLTYRELDLRADTLS
                     SRLRELGAGPESRVALFLERSADLLTGILAVWKAGASWVPLDPSQPDTRLALVLEDAF
                     RNQPAPLLIIHHSLRHRALPLSNAQTLFLDTTGRGADGGAGRPAQATGSVGAGEGGAH
                     TKEPSTAAPPRHPQGGDLAYVLYTSGTSGRPKGVMVEHGSLAHFVRSILLTFDFHPGD
                     RVPCVAPFTFDAFLLDVLPPLLTGGTAVIFDTRPPLDLPGLLDELESATHFDCVPALM
                     RQIVDGLRRRGASPTRLRWALMGGDAVPTDLLQAVRDVFPASRPTAVYGPTEATVVAS
                     FHFLGSSRWEDGRTLLGRPLPDALLELRNQEGHPVPIGVAGEVWLGGPGAARGYQNLP
                     DLTAAAWTVIDGRRWYRTGDLARRLPDGTVEFLGRVDQQVKVRGFRVEPGEIEAVLAG
                     VPGVGDVAVLALQDPAAPGEPRLVACVVPRSPEAGDLVGSLVEQARRRLPEYMVPADW
                     LVLPSFPVTPHGKLDRRALLALASAAGRTVAETPYEPPRTSTEELLADLWSQLLGRDG
                     TGRRDHFFDLGGHSLLAARLASRISDLFGMDLPLRLVFEAPRLEELARRIDATRAEEA
                     GPPAPPVVPVPRDRRLPLSFAQERLWFLDQLDPGQAVYNMPLAVRLAGGADPAVLAAS
                     LGELCRRHEALRTSFPSVEGAPYQAIAPAAPFVLPVIDLRGLPPDRAVREALSRLGEV
                     AARPFDLRRGPLLRTLAILESGGDLLLSLDLHHTVADGWSMGLLQGELVTLYGAVSRG
                     VPSPLPELPVQYADYAVWQRSWLSGEVLERQVAYWRRQLAGAPALLELPTDRPRPPVQ
                     SFRGGSLPLVLSPELTRSLQTVGRRRGATLFMTLLAAFQSLLHRASGQEVVPVGTPVA
                     NRGRTEVEGLIGLFVNTLVLATDLTGDPPFGELLARVRETSLEGQAHQELPFEKLVEV
                     LAPERSLGHAPLFQVMLTFQNLPAPAAPTPLAGEGALPSLALQPVETSLGLAKFDLSL
                     TLEEMEGGLAGSLSYAADLFDRTTAVRLAQQLEALLAAVAARPETRPSELLLLSPAQR
                     QQLLHEWSGAVRTERGAVSGRCLHQSVEAQVERTPDAVAVRFGADSLTYQELDGQANR
                     LARLLRRAGVGAEVPVAVFMDRSLEMVVALLGVLKAGGFYVPLDPAYPPERLAFMLED
                     CQARVLLSQDWLMERLPDTGVSRQIYLDPTFESLEDEDATRPEPLTVPAHLAYLIYTS
                     GSTGRPKGVAITHASAAALVDWAVDAFGEEELAGTLASTSICFDLSVFELFVPLSRGG
                     CVLVVETVLDLLENGGKIGGQPITLINTVPSAAAELVRSGLPASVRTVNLAGEPIPPL
                     LVGQIHAQAGVERLLNLYGPSEDTTYSTTEETAADAIVPPAIGRPIEGTRAYVLDGTL
                     HPVSPGLPGELYLTGAGLARGYFRRPDLTAE"
BASE COUNT          644 a         2091 c         1871 g          839 t
ORIGIN      
        1 ctcgagcgcg ggccgctgtt ccggccgtac ctgctgcggc tgtcggcgga ggaccacgtg
       61 gtcctggcct ccatgcacca catcgtcagc gacggctggt cgctgtcgat cctggtgcgc
      121 gagctggtgg ccctctacga agcgttccgt gccggccagg cctcgccgtt gccggagctg
      181 ccggtgcagt acgccgacta cgccgtctgg caacgccgct ggctcgctgg cgacgtcctg
      241 gaggcggagc tggcctggtg gcggcagcgg ctcgcgggcc tgccggcgct gctggagctg
      301 cccaccgacc ggccgcggcc gccggtgcag acctatgccg gcgacggccg gccggtgctc
      361 atcggacgcg agacgagcga ggccctggcc gggctggccc tcgcctccgg ggcgacgctc
      421 ttcatggcgc tgctcgccgt gttccagggc ctcctcggcc gctacgccgg gcagaccggg
      481 ctggcggtgg gctcacccat ggcggggcgt ggccgctcgg agctcgagcc cctcatcggc
      541 ttcttcgtca acaccctggt gctgccggcc gagctggcgg gccgtcccgg cttccgcgag
      601 ctgctggagc ggacccgccg gcggacgctc gaggcctacg cccaccagga ggtccccttc
      661 gagcgcctgg tggaggagct ggcgccggag cggagcctgg cctatccgcc gctcttccag
      721 gtcatgttcg tgctccagaa cacaccccgg gaggacctcc gcctgcccgg cctgcgtctg
      781 acctccatgg gctggggcga gacacggacc gccaagttcg acctgaccct ctcgctcacc
      841 gagaccggcg agggcctcgc cggcacgctc gaatacaaca ccgacctctt cgatggcacc
      901 accatcgacc gcatggccgt ccatctggag aggctgatcg cggccgcgct ggccgagccg
      961 gagacgccgc tggcggccct ctccttcctt tccgcgccgg agagccacca gctcaccgcc
     1021 gaatggaacg acacggcgat ctccaccccc gccggcctcc cggtccacct tcttttcgaa
     1081 tcccaggcgg cgcgcacgcc ggacgctccc gccgtctcct tcgccggcga ggtcctcacc
     1141 taccgggagc tggacctccg ggccgatact ctctccagcc gcctccgcga gctgggagcc
     1201 gggccggagt cccgggtcgc cctcttcctg gagcgctccg cggacctgct gaccggcatc
     1261 ctcgccgtct ggaaggccgg cgcatcctgg gtacctctgg acccatccca gcccgacacc
     1321 cgtctcgccc tggtcctcga agacgcattc cggaaccagc cggcgccgct cctcatcatc
     1381 catcactctc tccgccaccg ggccctccct ctttccaacg cccagaccct tttccttgac
     1441 acgaccgggc ggggggcgga tggcggggcg gggcggccag cgcaggcgac tggcagcgtg
     1501 ggggccgggg agggcggagc gcacacgaag gagccgagca cggccgcccc accccgtcat
     1561 ccgcaggggg gcgacctcgc ctacgtcctc tacacctccg gcaccagcgg ccgtcccaag
     1621 ggggtgatgg tggagcacgg cagcctcgcc cacttcgtgc gctccatcct cctgaccttc
     1681 gacttccatc caggcgaccg cgtcccctgc gtcgccccct tcaccttcga cgccttcctc
     1741 ctggacgtgc tcccgccgct gctgaccggt ggcacggcgg tgatcttcga cacccgtccg
     1801 cccctggacc tcccgggtct cctggacgag ctcgagtccg cgacccactt cgactgcgtg
     1861 cccgccctga tgcgccagat cgtcgatggc ctgcgccgcc gcggcgcctc cccgacccgg
     1921 ctgcgctggg ccctcatggg aggggacgcg gtcccgaccg atctcctcca ggcggtgcgc
     1981 gacgtcttcc ccgcgtcgcg gcccaccgcc gtctatgggc cgaccgaggc caccgtcgtc
     2041 gcctccttcc acttcctggg aagctcccgc tgggaagacg gccggaccct tctggggcgc
     2101 cccctgccgg acgcgctcct cgagctacgg aaccaggagg gccatcccgt acccatcgga
     2161 gtggcgggag aggtctggct ggggggcccc ggagccgctc gcggctacca gaacctcccc
     2221 gacctgaccg ccgcggcctg gacggtcatc gacggccggc gctggtaccg caccggggac
     2281 ctcgcccgcc ggctcccgga cggcacggtg gagttcctgg gccgtgtcga ccagcaggtc
     2341 aaggtgcgag gcttccgggt ggagccggga gagatcgagg ccgtgctggc cggggtaccc
     2401 ggcgtggggg acgtggcggt gctggcgctc caggatccgg cggcgccggg agagccccgc
     2461 ctcgtagcct gtgtcgtgcc gcggagcccg gaggccggcg atctcgtggg ctccctggtg
     2521 gagcaggccc gccgccgcct ccccgagtac atggtgccgg cggactggct ggtcctgccg
     2581 tcgttccccg tcacccccca cgggaagctc gaccggcgcg cgctgctcgc gctggcgtcg
     2641 gccgccggcc ggaccgtggc cgagaccccc tacgagccgc ctcgcacgtc caccgaagag
     2701 ctgctggcgg atctctggag ccagctcctc ggccgtgacg ggaccgggcg gcgcgaccac
     2761 ttcttcgatc tcggcgggca ctccctgctg gcggcgcgcc tggcctcccg gatcagcgac
     2821 ctcttcggca tggacctgcc gctccgcctg gtgttcgagg ctccgcggct ggaggagctg
     2881 gcgcggcgga tcgacgccac gcgcgccgag gaggccggcc cgccggcgcc gccagtcgtc
     2941 ccggtgcccc gcgaccgccg cctcccgctc tcgttcgccc aggagcgcct ctggtttctg
     3001 gatcagctcg atccagggca ggcggtgtac aacatgccgc tggccgtgcg gctggcgggc
     3061 ggagcggatc cggcggtcct ggccgccagt ctcggagagc tgtgccgccg tcacgaggcg
     3121 ctgcgcacct ccttcccctc cgtcgagggg gcgccctacc aggccatcgc gcccgccgcc
     3181 cccttcgtcc tgccggtgat cgacctgcgc ggcctgccgc cggaccgtgc cgtgcgggaa
     3241 gccctctcac gcctgggcga ggtggccgcg cggccgttcg atctgcggcg ggggccgctg
     3301 ctcaggacgc tggcgatcct ggagtccggc ggcgatctgc tgctgtcgct ggaccttcac
     3361 cataccgtgg ctgacggctg gtccatgggg ctcctccagg gcgagttggt cactctctac
     3421 ggcgccgtct cccggggcgt gccgtcgccg ctgcccgagc tgccggtcca gtatgccgac
     3481 tacgcggtct ggcagcggag ctggctgtcc ggcgaggtgc tggagagaca ggttgcctac
     3541 tggcggcggc agctcgccgg cgcgcccgcg ctcctcgagc tgcccaccga ccgcccgcgg
     3601 ccgccggtgc agagcttccg cggcggctcg ctgccgctgg tcctctcccc cgagctcacg
     3661 cgctccctcc agaccgttgg ccggcggcgc ggcgccaccc tcttcatgac tctcctggcc
     3721 gcgttccagt ccctcctcca ccgggcgtcc gggcaggagg tggtgccggt cggcacgccg
     3781 gtggcgaacc gcggccggac ggaggtcgag ggcctcatcg gtctcttcgt caacactctg
     3841 gtgctggcca cggatctgac cggcgacccg ccattcggcg aactgctggc ccgggtgcgc
     3901 gagacctccc tcgaaggcca ggcgcaccag gagctgccgt tcgagaagct ggtggaggtc
     3961 ttggcgccgg agcggagcct cggccacgcg ccgctgttcc aggtgatgct caccttccag
     4021 aacctcccgg cacccgctgc tccgacccct ctggccggcg agggagcgct cccgagcctc
     4081 gccctgcagc cggtggagac ctctctcggc ttggccaagt tcgacctgag cctcacgttg
     4141 gaggagatgg agggggggct cgccggctcc ctctcctacg ccgccgacct gttcgaccgg
     4201 acgacggccg tccgcttggc gcagcagctc gaggcgctcc tcgccgccgt cgccgcccgg
     4261 ccggagacca ggccctcgga gctgctcctc ctctccccgg cgcagcgcca gcagctcctg
     4321 cacgagtgga gcggtgccgt acgcaccgag cgcggggcgg tgtccggccg ctgtctgcat
     4381 cagtccgtcg aagcgcaggt ggagaggacg ccggacgccg tggccgtccg cttcggcgcc
     4441 gacagcctca cctatcagga gctggatggg caggcgaacc ggctggcccg cctgttgcgc
     4501 cgcgccggtg tgggcgccga agtaccggtg gccgtgttca tggaccggtc gctggagatg
     4561 gtcgtagccc tcctcggcgt gctcaaggcc ggcgggttct acgttccgct ggatcccgcc
     4621 tatccacctg agcgcctcgc cttcatgctg gaggactgcc aggcccgggt gctgctctcc
     4681 caggactggc tgatggagcg cctgccggac acgggagtgt cgcggcagat ctatctggac
     4741 cccaccttcg agtctctgga ggacgaggac gcgacgcggc cggagccgct gaccgttccg
     4801 gcccacctcg cctacctcat ctatacctcg ggctccacgg gccgtcccaa aggggtggcg
     4861 atcacccacg ccagcgccgc cgctctggtg gactgggcgg tggatgcctt cggcgaggag
     4921 gagctggccg gcaccctggc ctccacctcg atctgcttcg acctgtcggt gttcgagctc
     4981 ttcgtcccct tgtcgcgcgg cggctgcgtg cttgtcgtgg agaccgtgct ggatctgctg
     5041 gagaacggcg gcaagatagg agggcagccc atcaccctga tcaacaccgt gccctcggcc
     5101 gcggccgagc tggtccggtc cgggctgccg gcttcggtgc gcaccgtcaa cctggccggc
     5161 gagcccatcc cgccgctgct ggtggggcag atccatgccc aggctggcgt cgagaggctg
     5221 ctcaatctct atggaccgag cgaggacacg acctactcga cgacagagga gaccgcggcg
     5281 gacgccattg tcccgccagc gatcggccgg cccatcgagg gtacccgcgc gtacgtgctg
     5341 gacggcaccc tccacccggt ctcgcccggc ctgccggggg agctctacct gacgggcgcc
     5401 ggcctcgccc gcggctactt ccgccggccc gacctgacgg ccgag
//
LOCUS       DASPJI010000066        10529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_292844, whole genome shotgun sequence.
ACCESSION   DASPJI010000066 DASPJI010000000
VERSION     DASPJI010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10529)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10529
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292844"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="VEL74_01760"
     CDS             <1..1127
                     /locus_tag="VEL74_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61281.1"
                     /translation="GPLDNAYKEHLSEGRTSLFDTHPADRERITKSLGGPPQGVFACA
                     LPASVVFSDFASLERRVSRDLFQAFLGRELPDDAFVPAEKAVAAFAESQQDSAAAERY
                     LPGGVSLLHPLPLPGVLPAPPVDPLPALTGAVGSMEVGRKDAVAILERYSEIYNQRSQ
                     ANRAGSLLAYGVPIDAASFGLSGPKGGERNAVREAQSATTAQLEELARRLEPLQAAAA
                     HRLTLALSLLPGVAGRLEEGAAWQEQVPRLLDAAARLREVLPLLLDLQDTRALLAMVG
                     SLEDREGQLAGTLTELTRSGQTRLREMLASLEGAADPFPEEPIPLARLAAPDGVPAAE
                     PAPVYHAIGGTSALFDLYGRILGRLCRIAEGVEAAVAPRG"
     gene            1194..2729
                     /locus_tag="VEL74_01765"
     CDS             1194..2729
                     /locus_tag="VEL74_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015068270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HYG61282.1"
                     /translation="MKIPSLILKQMYTLGSLENAEDGVRFSLKNRLSNATLTRLTEVR
                     IDGQAVPLERLTLEMDGDRVLGSAIGPTKAVAFPLAKVMKVHAQTDQLPKGQHEISIA
                     FETTPFGSLSFKVKDAIAERVKRTTVPHDKDNDYTPEIIKARQKFVEEYTGIKLQHID
                     KFSFDPEVTKGNIENFTGVAQVPIGFAGPLEVNGEHAQGEFLVPLATAEGTLVASYNR
                     GMKVLNLSGGVTCTVVGDCMQRAPVFVFDNARDGRDFVKWVDANIDKIREEAEGTSSI
                     AKLLYIDRYLANKLVFLRFNFSTGDAAGQNMVGRATFAACSWILENHANIRHFYLESN
                     FATDKKASQINIMRTRGKRVTAEATIPRDVLIQHMRVDPETLAYHANIANVGAFLSGA
                     NNNGCHSPNGITAMFIATGQDVANVSESSAGVVYTEVTQAGDLYISITIPSLIVATYG
                     GGTGLATQRECLELLGCYGKGKVHKLAEIVAGVVLAGEISLAAAISSLDWVSSHEQYG
                     RNR"
     gene            2762..3058
                     /locus_tag="VEL74_01770"
     CDS             2762..3058
                     /locus_tag="VEL74_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61283.1"
                     /translation="MTKIEPRTGFRVFTLAGLLLTVLLLANSGLSPMTADPGPVCAAY
                     PSPYSCDTTYSGSPCDEWRDVGSSCGTYPDGTCKYCRVAAGTTPAGIMAVCVCS"
     gene            3235..4884
                     /locus_tag="VEL74_01775"
     CDS             3235..4884
                     /locus_tag="VEL74_01775"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HYG61284.1"
                     /translation="MPGFELLPDTPPHGLVRRFFTIHRHLFGLLFGSLVAHVREGQTS
                     RFRGRRGFRLLFSLERLLASLVRPFLDKSIVSHSVPVQLRRRLEILGPTFIKLGQVLA
                     LRRDLLPESITEELKNLLDRLPVVPFPRYLELIVEDQKRPIAEMYSWIDPQPIGSASI
                     AQTHRATTVEGDSVILKVVKPGIRETLTRDARLLKWLGSFLQIFLERYQPKRVMREFV
                     DYTLREVDLRREADNAETFAANFKDMPDVVFPAIYRQYSGRSVLCMSFLKGFKPSAPE
                     AVQLAPEDRDFLVDQGAASIIRMLFRDGFFHADLHPGNLLILSGPKVGFIDLGMVGRF
                     SEDLRRSLLYYYYTLVMGDPEGASRYLAAVAAPGPGADPVGFRREVTEVSQRWHQNAN
                     FKGFSLAQLVMQSVNLGAVYRMYFPVEMVLMVKALVTFEGVGQILKPGLDVAAVSQKH
                     VQTIFIDQFSPMTLAKQGLRNAPEVIQALAKAPMLITEGLRLLEQTTRRAPENPFAGL
                     RGTIFGGFCMVAGAVLAGAGGPWPVWGTLFAVGLIAALHRR"
     gene            4978..7785
                     /locus_tag="VEL74_01780"
     CDS             4978..7785
                     /locus_tag="VEL74_01780"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYG61285.1"
                     /translation="MTERPGRPSTRAMALFPFLLWCLSAFLAAQPGEPPRHAAPVNLG
                     FEQGDPGQTPPGWKVPEAAKAAGYTAAIDAGQAASGARSALLSGPAVDPRSFGNLMQS
                     VDAAPYRGQRVRYRAAVRIQSETGGWANLWLRIDRPGGALGFLDNMQERPITSAEWAS
                     YEIVADVAPDAESLNLGMMYRGGGRGWIDSVSLEVIGESGEGNEPPRALTGRGLENLI
                     AFTRLLGYVRFFHPSDQAGAADWNRVALAGVQAAEPAAGAAALARTLEDLFRPLAPTV
                     RVFPTGQTPPLPAELSRPAAGAARVVAWEHAGVDLSEDPMQRRYESHRADGRPRPGEF
                     GTISQHFDAAALRGKKVRFRAAVRAEVQGENQAQLWLRVDRPNGEMGFFDNMRDRPIR
                     QGEWNVYEITGDVAPDAQGIVAGVMLDQEGRVWIDEASLEAVGEEGRNLLQNGGFETD
                     APGAPPAGWSLPGRGDRSRYSFRVTEESPWRGRRSAVIAYTREEPSGDPGKPFVADLG
                     GGVSAMVPLALYADDRRTLPHSAADVTVKLAGKPEGFLPSGNDRTTRLAAVALAWNVF
                     QHFYPYFEEVQTDWPAVLRQSLHAAATDADEIAFGDTLRRLVAALQDGHGNVNGPSRP
                     RSHVPPLTWAWIEDRLVVTYVPGADSSGIRPGDVVVAIDGKPVRQVLEAAEALISSPT
                     PQWRRYIGLREIARGSEGEEVRLDIQPREGQPFTATLRRSATLRGPGSIEEPRPEKIA
                     EIRPGIFYLDVERANDGDFRAARDRLATAKGVIFDVRGYPSVTTHATLAHLTGEEMTS
                     AQWHVPLVTRPDREGMGFAVSNWAVKPAQPRFQGKVAFLTDGRAISYAETYMGIVEHY
                     KLGEIVGGPTAGTNGNINPFRLPGGYTINWTGMKVLKHDGSRHHGVGIQPTVPVSRTI
                     RGVAEGRDEVLEKAIEIVSRP"
     gene            complement(7811..8068)
                     /locus_tag="VEL74_01785"
     CDS             complement(7811..8068)
                     /locus_tag="VEL74_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61286.1"
                     /translation="MRMPFSPPLEHRRAAPRITSRALLVLGALLVIGGPVPLVLDIAF
                     RPEERNHLGLGLLVWLCWLAGGALLLAGGAGSVIARWRSGP"
     gene            8316..10430
                     /locus_tag="VEL74_01790"
     CDS             8316..10430
                     /locus_tag="VEL74_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG61287.1"
                     /translation="MKVPFLNIMDGDEGSSRLAGPEAEGRSGMGSAAPASREPTYFAD
                     PSHLAAEVTTPLPEASHPGLEGVLTAMADAFVILDRRWRFVFVNDRAAQIAGLTREEL
                     LGRVCWEVFPQSVGTVFHQELLRAASQQSVVRFEGFHSRLDGWLENRVYPFADGVAML
                     TSDITARKRAEEALRFLAEAEALLAGSLDYAETFRNLARLAVPRIADWCAVDLLDDPQ
                     TRRITVERADARLEALDAALGFVPAAVIARGETCWLPEVTDDALRGAGAGEEDVARLR
                     EAGLRSLVSVPLRPRSRTVGALTFAVARAGRCYGPDDLELAQGLAQVASLSLDNCRLF
                     QRVVEEDRRKDEFLAMMAHELRNPLAPIVNAVEMLRLRGEDPDLRRRATEMIARQARH
                     MARLLDDLLDVSRITYGKIGLRKERVDLVEVARRAFETSRPLFEARQHRVSLALPPEP
                     VWLDADPARLEQVLSNLLNNAAKFTPAEGRIAMEVAAGPDEAVVWVRDDGPGIPPELR
                     SRIFEPFVQEDRSLDRPSSGLGIGLTLVRTLVALHGGTAEVQSEGRGRGSEFTVRLPR
                     LPAEEEPEPGPVAPVPVGTPTLPRILLVEDNPDAAMALTDLLRLWGHEVAVAADGATA
                     LEIAGSFRPDVLLLDIGLPGMDGYELAHRLRLQEGLAAARFIALTGYGQETDRRRAAE
                     AGFDHHLTKPADPKVLRELLVG"
BASE COUNT         1566 a         3818 c         3653 g         1492 t
ORIGIN      
        1 ccgggccgct cgacaacgcc tacaaggagc acctcagcga ggggcggacg agcctgttcg
       61 acacccaccc ggccgaccgc gagcggatca ccaagagcct cggcgggccg ccccagggcg
      121 tgttcgcctg cgccctgccg gcgtcggtcg tgttcagcga cttcgcctcg ctcgagcgga
      181 gggtgtcgcg cgacctcttc caggccttcc tcggccgcga gctgccggac gacgccttcg
      241 tgccggcgga gaaggcggtg gccgcgttcg cggagagcca gcaggactcc gcagcggccg
      301 agcgctatct cccgggaggg gtgagcctgc tccatccgct ccccctgccg ggcgtcctgc
      361 cggcgccccc ggtcgacccg ctgccggcgc tgaccggggc ggtcgggtcc atggaggtcg
      421 ggcgcaagga tgccgtggcg atcctcgaac gctacagcga gatctacaac cagcggtccc
      481 aggccaaccg ggccggaagc ctcctcgcct acggggtgcc gatcgacgcg gcgtcgttcg
      541 gcctgagtgg tcccaagggt ggcgagcgga acgccgtgcg cgaggcccag agcgcgacga
      601 cggcgcagct cgaggagctg gcccgccgcc tcgagccctt gcaggccgcc gccgcgcacc
      661 gcctgacgct cgccctctcg ctcctccccg gcgtggccgg acgcctggag gaaggggcgg
      721 cctggcagga gcaggtgccg cggctgctgg acgccgcggc ccgtctgcgc gaggtcctgc
      781 cgctcctcct ggacctccag gacacgcggg cgctcctcgc catggtgggc agcctcgaag
      841 accgcgaagg ccagctcgcg gggacgctga cggagctcac gcggtcgggg cagacgcgcc
      901 tgcgggagat gctggcctct ctggaggggg ccgccgatcc gttcccggag gagcccatcc
      961 ccctcgcgcg cctcgccgcg cccgacggtg tcccggccgc cgagcccgcc ccggtctacc
     1021 acgccatcgg cggcaccagc gcgctgttcg acctgtacgg gcggatcctg ggacggttgt
     1081 gccggatcgc ggagggggtg gaggcggcgg tggctccccg cgggtgaggc cgcccccgtc
     1141 cgcctccctt ggggtagact tcccggcgca agaaacttcg cgaggtcatc ccgatgaaga
     1201 tcccgtccct gatcctgaag cagatgtaca ccctgggcag cctggagaac gccgaggacg
     1261 gcgtgcggtt cagcctcaag aaccggctct ccaacgccac cttgacccgg ctcaccgagg
     1321 tgcggatcga cggccaggcg gtccccctcg agcgcctgac cctcgagatg gacggtgatc
     1381 gggtcctcgg ctcggcgatc ggaccgacca aggccgtggc cttcccgctc gccaaggtga
     1441 tgaaggtcca cgcccagacg gatcagctcc ccaagggtca gcacgagatc tccatcgcct
     1501 tcgagaccac gccgttcggc tccctctcct tcaaggtcaa ggacgccatc gccgagcggg
     1561 tgaagcgcac cacggtcccg cacgacaagg acaacgacta cacgccggag atcatcaagg
     1621 cgcgccagaa gttcgtcgag gagtacaccg gcatcaagct gcagcacata gacaagttct
     1681 ccttcgaccc cgaggtcacc aaggggaaca tcgagaactt caccggcgtc gcccaggtgc
     1741 ccatcggctt cgccggcccg ctcgaggtca acggcgagca cgcccagggg gagttcctgg
     1801 tgccgctcgc caccgccgag gggaccctgg tcgcctccta caaccgcggc atgaaggtgc
     1861 tcaacctctc gggcggcgtc acctgcacgg tggtgggcga ctgcatgcag cgggcgccgg
     1921 tgttcgtctt cgacaacgcc cgcgacggcc gcgacttcgt gaagtgggtg gacgccaaca
     1981 tcgacaagat ccgcgaggag gcggagggca cctccagtat tgccaagctg ctctatatcg
     2041 accgctacct ggcgaacaag ctggtcttcc tgcgcttcaa cttctcgacc ggcgacgccg
     2101 cgggccagaa catggtgggc cgggcgacgt tcgcggcctg cagctggatc ctggagaacc
     2161 acgccaacat ccggcacttc tacctggagt ccaacttcgc caccgacaag aaggcgtcgc
     2221 agatcaacat catgcgcacc cggggcaagc gggtgacggc cgaggcgacg atcccgcgcg
     2281 acgtgctcat ccagcacatg cgggtggatc ccgagacgct ggcctatcac gccaacatcg
     2341 ccaacgtcgg cgccttcctc tccggggcga acaacaacgg ctgccactcg ccgaacggca
     2401 tcacggccat gttcatcgcc accgggcagg acgtggccaa cgtctccgag tcgtcggcgg
     2461 gggtggtcta caccgaggtg acgcaggcgg gcgacctcta catctcgatc accatcccgt
     2521 cgctgatcgt cgccacctac ggcggcggca cgggcctggc gacgcagcgc gagtgcctgg
     2581 agctgctggg ctgctatggc aaggggaagg tccacaagct ggccgagatc gtggccggag
     2641 tggtgctggc cggtgagatc tcgctcgcgg cggccatctc gtcgctcgac tgggtgtcga
     2701 gccacgagca gtacgggcgg aaccggtagg ccctcacctc tatcgacgaa aggagctgtc
     2761 gatgaccaag attgaacctc gtacgggctt tcgggtgttc accctcgccg gactgctgct
     2821 caccgtcctc ctgctcgcca acagcggcct gtcgccgatg accgccgatc ccggtccggt
     2881 ctgcgccgcg tacccttcgc cctacagctg cgacaccacg tacagtgggt caccctgcga
     2941 cgaatggagg gacgtcgggt cgagctgcgg cacctacccg gacggcacgt gcaaatactg
     3001 ccgggtggcg gcgggcacca cgccggccgg catcatggcc gtctgcgtct gttcctgagc
     3061 gtcccggcac tggatccggt ggcccgaggc gccggtcatg ccccccaagc tctcgccgag
     3121 gtgtccagaa agacgcgcga gagatacact ggagccatgc cggcgcaact tccagcggtg
     3181 gattcgaatt cggtagctcc tcccgcgccg gccgctgttg aaagcgccag cgggatgccc
     3241 ggcttcgagc tgctgccgga taccccgccc cacgggctcg tccggcgctt cttcaccatc
     3301 caccggcacc tcttcggcct gctcttcggg tctctcgtcg cccacgtgcg ggaggggcag
     3361 acgtcgcgct tccggggccg gcgcgggttc cgcctcctct tcagcctgga gcggctgctc
     3421 gcctccctgg tgcggccgtt cctggacaag tcgatcgtct cccactccgt gccggtgcag
     3481 ctccggcggc ggctggagat cctgggcccg acgttcatca agctgggcca agtgctcgcc
     3541 ctgcgccgcg acctgctgcc cgagtcgatc accgaggagc tcaagaacct gctggaccgg
     3601 ctgccggtgg tccccttccc ccgctacctc gagctgatcg tcgaggacca gaagcggccg
     3661 atcgccgaga tgtactcgtg gatcgacccg cagccgatcg gctcggcgtc gatcgcccag
     3721 acccaccgcg ccaccacggt cgagggggac tcggtcatcc tcaaggtggt caagccgggg
     3781 atccgtgaga cgctgacccg cgatgcgcgc ctgctcaagt ggctcggctc gttcctccag
     3841 atcttcctcg agcgctatca gcccaagcgg gtgatgcggg agttcgtcga ctacaccctg
     3901 cgcgaggtcg acctgcggcg cgaggccgac aacgccgaga ccttcgcggc caacttcaag
     3961 gacatgccgg acgtggtctt ccccgccatc taccggcagt acagcggccg cagcgtcctc
     4021 tgcatgtcgt tcctcaaggg cttcaagccg agcgcgcccg aggcggtcca gctcgcgccc
     4081 gaggaccggg acttcctggt ggaccagggg gcggcgagca tcatccgcat gctcttccgg
     4141 gacggcttct tccatgccga cctccacccg gggaacctgc tgatcctgtc cggccccaag
     4201 gtggggttca tcgacctggg gatggtgggg cgtttcagcg aggacctgcg gcgctcactc
     4261 ctctactact actacacgct ggtcatgggc gaccccgagg gggcgtcgcg gtacctcgcc
     4321 gcggtggccg cgcccgggcc cggagccgac ccggtagggt tccgccgcga ggtcaccgag
     4381 gtctcccagc gctggcacca gaacgccaac ttcaaggggt tctcgctggc ccaactcgtc
     4441 atgcagtcgg tgaacctggg cgccgtctac cggatgtact tcccggtcga gatggtgctc
     4501 atggtcaagg cgctggtcac cttcgagggg gtgggccaga tcctgaagcc ggggctggac
     4561 gtggccgcgg tgtcgcagaa gcacgtgcag accatcttca tcgaccagtt cagccccatg
     4621 acgctcgcca agcaggggct gcgcaacgcg cccgaggtga tccaggcgct ggccaaggcg
     4681 ccgatgctga tcaccgaagg cctgcgcctg ctggagcaga ccacccgccg ggcgccggag
     4741 aaccccttcg ccggcctgcg cgggacgatc ttcggcggct tctgcatggt ggccggcgcg
     4801 gtgctcgccg gcgccggcgg cccctggccg gtctggggca ccctgttcgc cgtcggcctc
     4861 atcgcggccc tccaccgtcg ctgagccgcc gctgagccgg cccgcagcac cctcgcgaaa
     4921 acaatccggc ctcctgctcc gtagaatgga gcagacgacg ttcgaggagc cttgccgatg
     4981 actgagagac cgggtcgtcc gagcacgcgc gccatggcgc tgttcccgtt cctgctctgg
     5041 tgtctgtccg ccttcctggc cgcccagccg ggcgagccgc cgcggcacgc ggcgccggtg
     5101 aacctcggct tcgagcaggg cgatccgggc cagacgcccc ccggctggaa agtccccgag
     5161 gcggcgaagg cggccgggta cacggcggcg atcgatgccg ggcaggccgc gagcggcgcg
     5221 cgctccgccc tcctctccgg cccggcggtt gatccccgca gcttcggcaa cctgatgcag
     5281 tccgtcgacg ccgcccccta ccgcggccag cgggtccgtt accgcgccgc ggtccggatc
     5341 cagagcgaga cgggcggctg ggccaacctc tggctgcgga tcgaccgccc cggcggcgcc
     5401 ctgggcttcc tcgacaacat gcaggagcgg cccatcacct ccgcggagtg ggcgagctac
     5461 gagatcgtcg ccgacgtcgc tccggacgcc gagagcctca acctcgggat gatgtaccgg
     5521 ggcggcggcc ggggctggat cgactccgtc tccctggagg tgatcggcga gtccggcgag
     5581 ggcaacgagc cgccccgggc gctgaccggc cggggcctcg aaaacctgat cgccttcacc
     5641 cgcctcctcg gctatgtgcg cttcttccac ccgagcgacc aggccggcgc cgcggactgg
     5701 aaccgcgtcg ccctcgccgg cgtccaggcc gccgagcccg ccgccggcgc cgcggccctg
     5761 gcccgcacgc tcgaagacct cttccgtccc ctggcgccga cggtgcgggt cttccccacc
     5821 ggccagaccc cgcccctgcc ggccgagctg tcccggccgg cggccggcgc cgcccgggtg
     5881 gtcgcctggg agcacgccgg cgtggatctc agcgaggacc cgatgcagcg ccgttacgag
     5941 agccaccggg cggacggacg gccgcggccg ggggagttcg gcaccatctc gcagcacttc
     6001 gacgccgctg cgctacgcgg caagaaggtg cgcttccggg ccgcggtgcg cgccgaggtc
     6061 cagggggaga accaagccca gctctggctg cgcgtcgacc ggccgaacgg cgagatgggg
     6121 ttcttcgaca acatgaggga ccgcccgatc cgccaggggg agtggaatgt ctacgagatc
     6181 accggtgacg tggcccccga cgcgcagggg atcgtcgccg gggtgatgct cgaccaggag
     6241 gggcgcgtct ggatcgacga ggccagcctg gaggccgtcg gcgaggaggg ccgcaacctg
     6301 ctccagaacg ggggcttcga aacggacgcg ccgggcgctc cgcccgccgg gtggtcgctt
     6361 ccgggaaggg gcgacaggtc gcgctactcc ttccgggtga ccgaggagag cccctggcgc
     6421 gggcggcgca gcgccgtcat cgcctacaca cgggaggagc cgtccggcga tcccggcaag
     6481 ccgttcgtcg ccgacctcgg cggcggcgtc tcggcaatgg tgccgctggc gctgtacgcg
     6541 gacgaccgcc gcaccctgcc ccactcggcg gcggacgtca ccgtcaagct cgccggcaag
     6601 ccggagggct tcctcccctc gggcaacgac cggacgacac gcctggcggc ggtggccctg
     6661 gcctggaatg tcttccagca cttctatccg tatttcgagg aggtccagac cgactggccc
     6721 gcggtgctgc ggcagtccct ccacgccgcc gccaccgacg ccgacgagat cgccttcggc
     6781 gacaccctgc ggcgcctggt ggccgccctc caggacggcc acggcaacgt caacgggccg
     6841 tcccgccccc gctcgcacgt gccgcctctc acctgggctt ggatcgagga ccgtctcgtg
     6901 gtgacctacg tcccgggggc ggacagctcg gggatccggc cgggtgacgt ggtcgtggcc
     6961 atcgacggca agccggtccg gcaggtgctg gaggcggccg aggcgctgat ctccagcccg
     7021 acgccgcaat ggcgccgcta catcggcctg cgggagatcg cccgcggctc cgagggggag
     7081 gaggtccggc tggacatcca gccgcgggag gggcagccgt tcacggccac gctgcggcgg
     7141 agcgccaccc tgcgtggacc gggcagcatc gaggagccgc ggccggagaa gatcgcggag
     7201 atccgtcccg gcatcttcta cctcgacgtg gaacgggcca acgacggcga cttccgggcg
     7261 gcccgcgacc ggctggccac ggccaagggg gtcatcttcg acgtccgcgg ctacccctcc
     7321 gtgaccactc acgccacgct cgcccacctc accggcgagg agatgacctc ggcacagtgg
     7381 cacgtgcccc tggtgacccg ccccgaccgg gaggggatgg ggttcgctgt ctcgaactgg
     7441 gcggtcaagc cggcccagcc gcgcttccag ggaaaggtgg ccttcctcac cgacggccgg
     7501 gccatcagct acgccgagac ctacatgggg atcgtcgagc actacaagct cggcgagatc
     7561 gtgggcggcc ccaccgccgg caccaacggc aacatcaacc cgttccggct ccccggcggg
     7621 tacacgatca actggacggg catgaaggtg ctcaagcacg acggctcgcg ccaccacggc
     7681 gtggggatcc agcccacggt gccggtctcc cgcacgatcc gcggcgtggc cgagggaagg
     7741 gacgaagtgc tggagaaggc gatcgagatc gtcagccggc cctgaccgag ggcaagggag
     7801 ggccggccgc tcaggggccg ctgcgccagc gggcgatgac cgagccggcg ccgcccgcca
     7861 gcagcagcgc tcccccggcg agccagcaga gccagaccag gagtcccagg ccgaggtgat
     7921 tcctctcttc cgggcggaag gcgatgtcca ggacgagagg aaccgggcct ccgatcacca
     7981 ggagagcgcc gagcacgagc agggcccggc tcgtgatccg gggcgccgcc cgccggtgtt
     8041 ccaggggtgg agaaaaaggc atgcgcatgg gtatcccgcc tcgcgtcgct ctcaatctac
     8101 tcccggctcc cacgggtgtc aacgcgaggg gactggtgac gtttgtccat gaaatgggcc
     8161 ccggcgccaa ctcactatta gatgaagagt ggattgcgag cgatgctcgc ggcgtccagc
     8221 caggaggctc tgacctctgg ccatgagttc tttagaactt tctattgtag gatgtagcca
     8281 tcgacgccgc cgtatcctcg ggagagggga gcggcgtgaa agttcctttt ctcaacatca
     8341 tggacggcga cgaagggtca agccggctgg caggcccgga ggccgagggg aggtccggga
     8401 tgggaagcgc cgcgcccgcc agccgagagc cgacctactt cgccgaccct tcccacctcg
     8461 cggcggaggt caccaccccg ctgccggaag cttcacaccc cggcctcgag ggcgtcctta
     8521 cggcgatggc cgacgccttc gtaatcctcg accgccggtg gcggttcgtc ttcgtcaacg
     8581 accgggcggc ccagatcgcc ggcctgacgc gggaggagct gctgggccgg gtgtgctggg
     8641 aggtcttccc gcagtcggtg ggcactgtct tccaccagga gctgctccgc gccgccagcc
     8701 agcagagcgt ggtgcgcttc gagggcttcc actcgcggct cgacggctgg ctcgagaacc
     8761 gggtctaccc cttcgccgac ggggtggcga tgctgacgtc ggacatcacc gcccgcaagc
     8821 gcgccgagga ggcgctacgc ttcctcgccg aggccgaggc gctcctcgcc ggctcgctgg
     8881 actacgccga gaccttccgc aacctggccc ggctcgccgt cccccgcatc gccgactggt
     8941 gcgccgtcga cctgctggac gatccgcaga cgcgccggat cacggtggag cgcgccgacg
     9001 cccgcctcga agcactggac gccgccctcg ggttcgtgcc cgccgccgtg atcgcccgcg
     9061 gtgagacctg ctggctgccc gaggtcaccg acgacgccct gcgcggggcc ggcgccggcg
     9121 aggaggacgt ggcgcggctg cgcgaagccg ggctgcggtc gctggtgagc gtgccgctac
     9181 ggccgcggag ccggacggtg ggagccctga cgttcgccgt cgcccgggcc ggccggtgct
     9241 acggcccgga cgacctggag ctggcccagg gcctcgccca ggtggcttcg ctctctctgg
     9301 acaactgtcg tctcttccag cgggtcgtcg aggaggatcg ccgcaaggac gagttcctgg
     9361 ccatgatggc ccacgagctg cgcaatcccc tggcccccat cgtcaacgcc gtggagatgc
     9421 tccgcctgcg gggcgaggat cccgacctgc gccggcgggc aacggagatg atcgcgcgcc
     9481 aggcccgtca catggcgcgg ctgctcgacg acctcctgga cgtgtcgcgg atcacctacg
     9541 gcaagatcgg gctccgtaag gaaagggtcg acctggtgga agtggcgcgc cgggcgttcg
     9601 agaccagccg gccgctcttc gaggcgcgcc aacaccgggt gagcctggcg ctgccgccgg
     9661 agccggtctg gctcgacgcc gacccggcgc gcctggagca agtgctctcg aacctgctca
     9721 acaacgcggc caagttcacc cccgccgaag ggaggatcgc catggaggtc gcggccggcc
     9781 cggacgaggc ggtggtgtgg gtgcgggacg atgggccggg cattcctccg gagctccgca
     9841 gccggatctt cgagcccttc gtgcaggagg accgctccct ggaccgcccg tcgagcggcc
     9901 tcggcatcgg actcacgctc gtgcggaccc tggtggccct gcacggcggc accgccgagg
     9961 tacagagcga aggccggggc cggggcagcg agttcaccgt ccgcctcccc cgcctgccgg
    10021 cggaggagga gccggagccc ggaccggtcg caccggtccc tgtggggaca ccgacgctgc
    10081 cgcgcatcct cctggtggag gacaatccgg acgccgccat ggcgctcacc gacctcctcc
    10141 gtctctgggg ccacgaggtg gccgtagcgg cggacggcgc cacagcgctg gagatcgccg
    10201 gcagcttccg gccggacgtc ctgctgctgg acatcggcct cccggggatg gacggctacg
    10261 agctggcgca ccggctgcgg ctccaggagg ggctcgccgc cgcccgcttc atcgccctca
    10321 ccggctacgg ccaggagacc gaccgccgcc gcgccgccga ggcaggcttc gaccaccacc
    10381 tgaccaagcc cgccgacccc aaggtgctgc gggagctgct ggtggggtga gagggacgcc
    10441 cctcaaccct ggaccggccc gccgatctcg ccgagccggt cccgcacgcg ccgggtgagg
    10501 gggtggtcct cccccatcac ctgctggcg
//
LOCUS       DASPJI010000067         3212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_464261, whole genome shotgun sequence.
ACCESSION   DASPJI010000067 DASPJI010000000
VERSION     DASPJI010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464261"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..461)
                     /locus_tag="VEL74_01795"
     CDS             complement(117..461)
                     /locus_tag="VEL74_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61288.1"
                     /translation="MPSFSDRTPSRVDLSRPTESTRRVLYELVVRVYFHPTPEEVEKA
                     RQDPGDHWVPDYGPDEARLHVFHVYGRWFAVWEALEEKEAGLPEWRRWSVVRIVEDRD
                     PSSATGVTFLEV"
     gene            972..1991
                     /locus_tag="VEL74_01800"
     CDS             972..1991
                     /locus_tag="VEL74_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016335711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYG61289.1"
                     /translation="MEWSQKRILVTGGASFISSHLIDLLVEKGARQIRVVDDLTSGKL
                     ANIQEHIDAGRVEFRQEDLLGPGVPQRAVEGVDVVFHLAAIHGGRGYVDLHQAACAQN
                     LAMDGMLIKAAHEAGVEKFVFASSGCVYPNFLQTDVTKELYLTEEMVGPPYDADNMYG
                     WAKLMTEMTLQSYYKDFGFKGASCRFFTVYGERGVENHAVIAMIARAFVRQNPFEVWG
                     DGTQIRNWTYVGDIVRGMVMAAEKIDDGTAVNLGTEERTRVIDAVREVLRYTGQEAEI
                     KFLPHMPTGPLNRVASNRLARELMGWEPQVKFMDGLHRTIDWYFGSRQRETVSGYLEE
                     MLTER"
     gene            2002..3120
                     /locus_tag="VEL74_01805"
     CDS             2002..3120
                     /locus_tag="VEL74_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61290.1"
                     /translation="MRKLVYLCDWLPPDFGAVGQYSLLFARQRAAAGEEVTLGGLSST
                     AGSVEEESAGAGRLRIVRLHAPTYDRADFKTRALWTARTNLALVRRLRREIRQADEVL
                     FTGSPPFLIHLLVPLNLLFRKRLTYRITDFHPECLMAEIGEMGKVPAPLRLFHRLTVA
                     LRRRVHRFEVLGEDQRQRLLEIGIPDERIVLKRDPSPVEIPEGTPPLERPAELAGRCL
                     LLYSGNFGVAHDHETFVAGYRLHHRQGSGRVGLWLNATGAKADLVEKALREEGLPVHR
                     GRPVPLDLLPRLLVTPDAHLITLRDEFTGYVLPSKVHACLQSGRSILYIGPAASDVHL
                     LGAREMPPGLYRRVGTGDAEGAARALEEIADGCATSPG"
BASE COUNT          506 a         1125 c         1074 g          507 t
ORIGIN      
        1 gctgccccat gaggaatgat ccccgccgcc gcttccgcgg cgacggggat ctcttccttg
       61 tcgtcagggg gagagggggc gggccccgag aggcgggacc cgccgtccgg gcggcgtcag
      121 acctcgagaa aggtgacgcc cgtggcgctc gacgggtccc ggtcctcgac gatccgcacc
      181 accgaccagc gccgccactc cggcagcccg gcctccttct cctccagcgc ctcccagacc
      241 gcgaaccagc ggccgtagac atggaagacg tggaggcgtg cctcgtcggg accgtagtcc
      301 ggcacccagt ggtcgccggg gtcctgacgg gccttctcca cttcttcagg cgtgggatgg
      361 aagtagacgc ggaccaccag ctcgtagaga acgcggcggg tggattcggt gggacgggac
      421 agatcgacgc gcgaaggcgt acgatcactg aagctgggca tgaggttaag ctcctgtccg
      481 gctaggcctc gggaggcgag ctcgtaactc cctcccttgg ccgtctgtag ggctccaggt
      541 gaggcacgcg ctcacctcgg agacacaacg gctgtgctgg gaagcctcgg cttccttact
      601 cttcagactc tctccccggc cacctccttt ccgcattgat tacggctgca ccgagcctat
      661 accgggtttt ggagagaggt caagaggaag gtggaagaat cttgcggggg agttttgggt
      721 tgcgggggcg gaggaaaaac ccccctcctt gacctccccc gctcgctacc gcccaccctc
      781 ccagggaggg aggcaccacc gccccctatt ccgggctgtc cgtgatggtc cgtgctcgtc
      841 cgtgccagtc cgtgtgtccg tgctccctgc gcctgtacac gcgccggctc gtcgtttaga
      901 cttagcgctc ctctgaagag cacatgtcgc acgaaggctc ctcttggaag ccgtgcagga
      961 gagtatcgag tatggagtgg tcgcagaaaa ggatcctggt caccggcgga gcctcgttca
     1021 tcagctccca ccttattgat ctgctggtcg agaagggggc gcgccagatc cgcgtcgtcg
     1081 acgacctgac gagcggcaag ctcgccaata ttcaagagca catcgacgcc gggcgggtcg
     1141 agttccggca ggaggacctc ctgggccccg gcgtgccgca gcgggcggtc gagggggtcg
     1201 acgtcgtctt ccacctcgcc gccatccacg gtggccgcgg ctacgtcgac ctgcaccagg
     1261 cggcctgcgc gcagaacctg gcgatggacg gcatgctgat caaggccgcc cacgaggcgg
     1321 gcgtcgagaa gttcgtcttc gcctcctcgg gctgcgtcta ccccaacttc ctgcagaccg
     1381 acgtgaccaa ggagctctac ctcaccgagg agatggtcgg gccgccctac gacgccgaca
     1441 acatgtacgg ctgggcgaag ctgatgaccg agatgaccct ccagtcctac tacaaggact
     1501 tcggcttcaa gggggcctcg tgccgcttct tcacggtcta cggcgagcgc ggggtggaga
     1561 accacgccgt catcgccatg atcgcccgcg ccttcgtgcg ccagaacccg ttcgaggtct
     1621 ggggcgacgg gacccagatc cgcaactgga cctacgtcgg cgacatcgtc cgcggcatgg
     1681 tgatggcggc cgagaagatc gatgacggca cggcggtcaa cctggggacc gaggagcgga
     1741 ctcgggtcat cgacgcggtg cgcgaggtgc tgcgctacac cggccaggag gcggagatca
     1801 agttcctgcc ccacatgccc accgggccgc tcaaccgggt ggcgagcaac cgcctcgccc
     1861 gcgagctgat gggctgggag ccgcaggtca agttcatgga cggcctgcac cgcaccatcg
     1921 actggtactt cgggagccgc cagcgcgaga cggtcagcgg ctacctggag gagatgctga
     1981 cggaacggta gccgtcccgc cgtgcgcaag ctggtctatc tctgcgactg gctccccccg
     2041 gacttcgggg cggtcggtca atacagcctc ctcttcgccc gccagcgcgc cgccgccggc
     2101 gaggaggtga cgctgggcgg cctctcctcc accgccggct cggtggagga ggagagcgcc
     2161 ggcgccggcc ggctgcgcat cgtccgcctg cacgccccca cctacgaccg ggccgacttc
     2221 aagacgcggg cgctgtggac ggcgcgcacc aacctcgccc tggtgcggcg gctgcggcgc
     2281 gagatccggc aggcggacga ggtgctgttc accggcagcc cgccgttcct gatccacctg
     2341 ctggtgccgc tcaacctgct gttccgcaag cggctcacct accgcatcac cgacttccat
     2401 cccgagtgcc tgatggctga gatcggcgag atggggaagg tgccggcgcc gctgcggctg
     2461 ttccaccggc tgacggtggc gctgcgccgc cgggtccacc gcttcgaggt gctgggggag
     2521 gaccagcggc agcggctgct ggagatcggc atcccggacg agcgcatcgt cctgaagcgc
     2581 gacccctcgc cggtcgagat cccggaaggc accccgccac tcgagcggcc ggcggagctg
     2641 gcgggccgct gcctgctcct ctactcgggc aacttcggcg tcgcccacga ccacgagacc
     2701 ttcgtcgccg gctatcgcct gcaccaccgg caaggctccg gacgggtcgg gctctggctc
     2761 aatgccaccg gcgccaaggc ggacctcgtg gagaaggcgc tacgcgagga ggggctcccc
     2821 gtccaccgcg gccggccggt gccgctggac ctcctgccac gcctgctggt cacccccgac
     2881 gcccacctga tcaccctgcg cgacgagttc accggctacg tcctaccgtc caaggtccac
     2941 gcctgcctcc agtcgggccg gagcattctt tacatcggcc cggccgcgtc ggacgtccac
     3001 ctgctcggcg cgcgcgagat gccccccggc ctctaccggc gcgtcggcac cggggacgcc
     3061 gagggggcgg cccgggctct cgaagagatc gccgacggtt gcgcaacctc tccaggttga
     3121 atccccgccc gggtttagag taagatccac ccgcaaatcc cgtcagttgt tgaaagttcg
     3181 gcctccccat ccgttcggga gagccagggg ag
//
LOCUS       DASPJI010000068         5225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_118493, whole genome shotgun sequence.
ACCESSION   DASPJI010000068 DASPJI010000000
VERSION     DASPJI010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118493"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="VEL74_01810"
     CDS             complement(<1..131)
                     /locus_tag="VEL74_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HYG61291.1"
                     /translation="MIDAIGVFDSGVGGLTVAAALRRRLPHESILYLGDTARLPYGS"
     gene            complement(128..811)
                     /locus_tag="VEL74_01815"
     CDS             complement(128..811)
                     /locus_tag="VEL74_01815"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HYG61292.1"
                     /translation="MSRRAAGAILGLALVVLTVGVTWWLLSDRGSRPWRRGPQEEAAQ
                     PREKVSFELYFPAGPSGNAMLRGERRELEVTESPRDRVRKIVEALLAGPRQPGLARPF
                     PEQVQLGSVQLVPGASEETGNIAYVDLRWAEQAEPPAGGSTEEIQRIYSVVDSVALNV
                     PEVQGVVLLWNGTQRATFSGHLDTSLPLPPDRTLVAPAGAAAPAQPAPSPQAAPTPTP
                     ATPAGQPAQ"
     gene            complement(808..2421)
                     /locus_tag="VEL74_01820"
     CDS             complement(808..2421)
                     /locus_tag="VEL74_01820"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HYG61293.1"
                     /translation="MKARRSRVPLLALCLSLPVLVAAQIAQTPPAPPVPQAGGPAPAS
                     PGRPTVVLGGRDLQVPVTIRPAGPVVALAPLAQALGGELSPDESGESSTLRIGETDIV
                     LGVGSAIVTVGDQIVSLSQPVTRGEAGLEVPIDFLSKTWGDLSGYSFDWRPQEGRLAI
                     GTRSAREIPVALDVVHLQAMTTVVLQFPEAPRYDLVQQPGVIEVRMLEDRLAPPADLP
                     QVKDPLVQGVSITPQGVRLQLTPGAAVESYVLENPFRLVFDVHQPSTVTVPGGPPVTE
                     IRDEGGIRTIVIDPGHGGTETGAIGPSGVAEKELTLILARDLEARLEQRLPVRVILTR
                     NEDANLHWDTRAAIANQNRADLFISVHLNSSLGSGARGAETYFLSTQASDARAARAAE
                     AENQGDATPPPGAEGLDAETQQDLQMILWDLAQTRHLTESQRFAGMVQAELNQTLQIR
                     DRGVKQAPFRVLVGADMPAVLVELGFLSNPEEETKLQDPAYRSQLIDALVQAIARYKQ
                     TVDAAEAASAPAPPGTAPRATPGATPGTR"
     gene            2532..2924
                     /locus_tag="VEL74_01825"
     CDS             2532..2924
                     /locus_tag="VEL74_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYG61294.1"
                     /translation="MEIRQFRVVVRSRYFERALKFYGDSLSLPRLHAWDREDGRGAVF
                     QAGTAVVEVLGAPADEDPRMRNEEFDPQGPTGKMSLVILVPSAQQAYDEIFFRDKNIP
                     GGLRKDYDGALVFETHDPDGIRVLFKEG"
     gene            complement(2937..4568)
                     /locus_tag="VEL74_01830"
     CDS             complement(2937..4568)
                     /locus_tag="VEL74_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYG61295.1"
                     /translation="MPLRLVATTGDSSFRVPPGRALSVGRTADCDLPVRDPTVSRQHA
                     EVECVGNRLHIRDGGSTNGTFVDGRRVHDAVALPGARIAFGKVTFELREEPALDSDVQ
                     LPVEDPLDATILRQVQVVGHANIAAHLGGAPAARGTPADRQAERLALMLDIARELSGP
                     TGLSEIDRLLEKVVDILFQVMSVDRVAILMAEPGSGEGGAPGGQPEGDLGELVPRVWR
                     SRVDGAFGGSWRVPKAVARKTVTERVAMLLENFPAADGQSEGATLAIPNIRSALCAPL
                     LGQQGRVLGLVYLDNLAADHTFTDEDLGFLTAFAGMAAVSIENSQLLQRVQREAVVLS
                     NFQRYFAPDLARQIAAQTGEIGLGGSRRTVTVLFCDIRGFTSLSEQMAADEIAKLLTE
                     YFTEMVDVVFEHGGILDKFMGDALMALWGAPVERDGDADRAVRAAVAMQQAVQQLNFE
                     WVGQGRRTISVGIGLSQGEVFAGNIGSDRRLEYTVIGDAVNTAARLCAEAGPGDILIA
                     EPLHASLTTPPRVRALPPLPLKGKAQAVPVYRVEW"
     gene            4732..4807
                     /locus_tag="VEL74_01835"
     tRNA            4732..4807
                     /locus_tag="VEL74_01835"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4766..4768,aa:Glu,seq:ctc)
     gene            4852..>5225
                     /locus_tag="VEL74_01840"
     CDS             4852..>5225
                     /locus_tag="VEL74_01840"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HYG61296.1"
                     /translation="MAPEAPPPKLIADAMLGALARWLRALDLDVAYDPALDDPELVAR
                     SAAEGRTILTRDRRLVERRLARNHLLIRSEKVEEQVRQVLGELAIHPEPGRLLGRCLR
                     CNLPLEPLPAERARARVPPWVAR"
BASE COUNT          677 a         1848 c         1951 g          749 t
ORIGIN      
        1 ttgctgccgt agggcaggcg cgccgtgtcc cccagataga ggatggactc gtggggcagc
       61 ctgcggcgca gggccgccgc caccgtgagg ccgcccacgc ccgaatcgaa gacaccgatg
      121 gcgtcgatca ctgagcgggc tgtccggccg gcgttgccgg ggtcggcgtc ggcgcggcct
      181 ggggagacgg ggccggctga gcgggcgccg ccgccccggc cggcgctacg agcgtccggt
      241 cgggcggcag ggggaggctg gtgtcgaggt ggccggagaa ggtggcgcgc tgggtgccgt
      301 tccagagcag cacgaccccc tgcacctcgg ggacgttgag cgccaccgag tcgacgacgc
      361 tgtagatccg ctggatctcc tcggtcgagc cgccggcggg gggctccgcc tgttcggccc
      421 agcgcaggtc gacataggcg atgttgcccg tctcttcgga agcgccggga acgagctgga
      481 cgctgccgag ctgcacctgc tccgggaacg ggcgggcgag gccgggctgg cgcggcccgg
      541 cgagcagcgc ctcgacgatc ttgcggacgc ggtccctcgg ggactcggtg acctcgagct
      601 cgcgccgctc gccgcgcagc atggcgttgc cggatgggcc ggccgggaag tagagctcga
      661 aggacacctt ctcccgcggc tgggcggcct cctcctgcgg cccgcggcgc caggggcggc
      721 tccccctgtc gctcagcagc caccaggtca cgccgaccgt gaggacgacc agcgccagcc
      781 cgaggatcgc tcccgccgcc cgccggctca tcgcgtcccc ggcgtggcgc ccggcgtcgc
      841 tctgggagcc gtccccggag gagcgggggc cgacgccgcc tccgcggcgt ccaccgtctg
      901 cttgtagcgg gcgatcgcct gcaccagcgc gtcgatgagc tgcgaccggt aggcgggatc
      961 ctggagcttg gtctcctctt cggggttgct gaggaagccc agctcgacca ggacggcggg
     1021 catgtccgcc cccaccagga cgcggaaggg ggcctgcttg accccgcggt cccggatctg
     1081 gagggtctgg ttgagctccg cctggaccat gccggcgaag cgctggctct cggtcaggtg
     1141 gcgggtctgg gcgaggtccc agaggatcat ctggagatcc tgctgcgtct ccgcgtccag
     1201 cccctcggcg ccgggcggcg gagtcgcgtc gccctggttc tcggcctcgg cggccctggc
     1261 ggcccgggcg tcggacgcct gggtgctcag gaagtaggtc tcggcgccgc gcgccccgga
     1321 gcccagagag gagttcaggt gcacggagat gaagaggtcg gcgcggttct ggttggcgat
     1381 ggcggcgcgg gtgtcccagt ggaggttggc gtcctcgttg cgggtgagga tcacgcgcac
     1441 gggcagccgc tgctccaggc gcgcctcgag gtcgcgggcg aggatcagcg tcagctcctt
     1501 ctcggcgacg ccgctggggc cgatcgcccc ggtctcggtg ccgccgtggc cggggtcgat
     1561 gacgatggtg cggatgcccc cctcgtcccg gatctcggtg accggcggcc ctcccgggac
     1621 cgtcaccgtc gacggctggt ggacgtcgaa caccagccgg aacgggttct ccagcacgta
     1681 gctctccacc gcggcgcccg gggtgagctg cagccgcacc ccctgcggcg tgatgctcac
     1741 cccctggacc agcgggtcct tcacctgcgg cagatccgcc ggcggcgcca ggcggtcctc
     1801 cagcatgcgg acctcgatca cccccggctg ctgcaccagg tcgtagcgcg gcgcctcggg
     1861 gaactgcagc accaccgtgg tcatcgcctg gaggtgcacg acgtcgagag cgaccgggat
     1921 ctcccgcgcg ctgcgggtgc cgatggccag gcgcccctcc tgcggccgcc agtcgaagct
     1981 gtagccggag aggtcgcccc aggtcttgct caggaagtcg atcggcacct cgaggccggc
     2041 ttcaccgcgg gtgaccggct gcgacagcga gacgatctgg tcgccgacgg tgacgatggc
     2101 gctgccgacg ccgaggacga tgtcggtctc gccgatgcgc agggtcgagc tctcgcccga
     2161 ctcgtccggc gacagctcgc cccccagcgc ctgggccagc ggggccagcg ccaccaccgg
     2221 gccggcgggt cggatggtga ccggcacctg gaggtcgcgg ccgccgagca ccacggtggg
     2281 gcggccgggg gacgcggggg ccgggcctcc tgcctggggg accggcgggg ccggaggggt
     2341 ctgcgcgatc tgggcggcga cgaggacggg cagcgacagg cagagcgcga gcagggggac
     2401 gcgagaacgg cgtgctttca cgcgggagag gataccaccc ggggggcgtc cctttgaaag
     2461 tggctcgccg agtgtggtaa tgctctgctt tcccggggcc gtcccggcgt ctatccaagg
     2521 agaggaaagc gatggagatc cggcagtttc gggtggtggt ccgctcgcgt tacttcgagc
     2581 gggcgttgaa gttctacggc gattccctgt ccctgccgcg gctccacgcc tgggaccgcg
     2641 aggacggccg cggcgccgtc ttccaggccg ggactgccgt cgtcgaggtg ctcggcgcgc
     2701 cggcggacga ggatccgcgg atgcgcaacg aggagttcga tccccagggg ccgaccggca
     2761 agatgagcct ggtgatcctc gtcccctcgg cgcagcaggc ctacgacgag atcttcttcc
     2821 gggacaagaa catccccggc ggcctgcgca aggactacga cggcgccctg gtcttcgaga
     2881 cccacgaccc ggacggcatc cgggtgctgt tcaaggaggg gtgatcccgg cgccgctcac
     2941 cactcgaccc ggtagaccgg caccgcctgc gccttgccct tgagcggcag cggcggcagc
     3001 gcccggaccc gcggcggggt agtgagggag gcgtgcagcg gctcggcgat caggatgtcc
     3061 cccgggcccg cctcggcgca gaggcgggcc gcggtgttca ccgcgtcgcc gatcaccgtg
     3121 tactccaggc gccggtcgct gccgatgttg ccggcgaaga cctccccctg gctgaggccg
     3181 atcccgacgg agatggtccg ccgcccctgg cctacccatt cgaagttgag ctgctgcacc
     3241 gcctgctgca tggcgaccgc cgcgcgcacc gcgcggtcgg cgtcgccgtc gcgctcgacc
     3301 ggcgcccccc agagggccat gagggcgtcc cccatgaact tgtcgaggat gcccccgtgc
     3361 tcgaagacga cgtccaccat ctcggtgaag tactcggtga ggagcttggc gatctcgtcc
     3421 gccgccatct gctccgagag tgaggtgaac ccccggatgt cgcagaacag caccgtcacc
     3481 gtgcgccgcg agccgccgag gccgatctcg ccggtctgcg ccgcgatctg gcgcgcgagg
     3541 tcgggggcga agtagcgctg gaagttggag aggacgaccg cctcgcgctg cacccgctgc
     3601 aggagctggc tgttctcgat ggataccgcc gccatgccgg cgaaggcggt gaggaagccg
     3661 aggtcctcgt cggtgaaggt gtggtccgcg gccaggttgt cgagatagac gaggccgagc
     3721 accctcccct gctggccgag cagtggcgcg cacagggcgc tgcggatgtt ggggatcgcc
     3781 agcgtcgccc cctccgactg gccgtcggcg gcggggaagt tctcgagcag catggcgacg
     3841 cgctcggtca ccgtcttgcg ggccaccgcc ttgggcaccc gccagctgcc gccgaacgcc
     3901 ccgtcgacgc ggctgcgcca gacccgcggc acgagctcgc ccaggtcgcc ctcgggctgt
     3961 ccgccggggg cgccgccctc cccgctgccg ggctcggcca tgaggatcgc cacgcggtcg
     4021 acgctcatca cctggaagag gatgtcgacc accttctcga gcagccggtc gatctcgctg
     4081 agccccgtcg ggccgctgag ctcgcgggcg atgtcgagca tcagggcgag ccgctcggcc
     4141 tggcggtcgg ccggggtgcc acgggccgcg ggcgcgccgc cgaggtgggc cgcgatgttg
     4201 gcgtgcccca ccacctgcac ctggcggagg atggtggcgt cgagcgggtc ctccaccggg
     4261 agctgcacgt cggagtcgag ggcgggctcc tcgcgcagct cgaacgtcac cttgccgaag
     4321 gcgatccgcg cccccggcag cgccaccgcg tcgtgcaccc gccggccgtc gacgaacgtg
     4381 ccgttggtgg agccgccgtc gcggatgtgc agccggttgc cgacgcactc gacctcggcg
     4441 tgctggcgcg acaccgtggg gtcgcgcacc ggcaggtcgc agtccgcggt ccggccgacg
     4501 ctcagcgccc gccctggggg gacgcggaac gaggagtctc cggtggtggc gacgagtctc
     4561 agcggcacgg tgttccctcg gtggcccgaa agcgggcgtc tccttgacgt ctacgctact
     4621 acagggacgt gacgacctgg ataacgtgaa gggcccgagc gcgggaatgt cctggggggt
     4681 tgaatcgttt tttggggctg tggtaccctt cgcgggtccg ttggacggca tggcgcattc
     4741 gtctaggggt ccaggacgcc ggcctctcac gccggtaaca cgggttcgaa tcccgtatgc
     4801 gctaccaaac ctgacacact ccccagcccg gcgccgccgg gcttttgaat tttggccccg
     4861 gaagcacctc cccccaagct catcgccgac gccatgctgg gcgccctcgc ccgctggctg
     4921 cgcgccctgg atctcgacgt cgcctacgac ccggcgctcg acgaccccga gctggtggcc
     4981 cgctccgcgg ccgaagggcg gaccatcctc acccgtgacc gccgcctcgt cgagcggcga
     5041 ctcgcccgca accacctgct gatccgctcc gagaaggtgg aggaacaggt gcggcaggtc
     5101 ctcggcgagt tggcgatcca tcccgagccc ggccgtctcc tcggccgctg cctgcgctgc
     5161 aatctgccgc tggagccgct gccggcggag cgggcgcggg cccgggtccc cccgtgggtg
     5221 gcgcg
//
LOCUS       DASPJI010000069         4628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_805376, whole genome shotgun sequence.
ACCESSION   DASPJI010000069 DASPJI010000000
VERSION     DASPJI010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805376"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1477)
                     /locus_tag="VEL74_01845"
     CDS             complement(98..1477)
                     /locus_tag="VEL74_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG61297.1"
                     /translation="MASSVRETLERHLEEAGERGAPKPAMPFERRVLLWALITGLPGV
                     SIALLLLWWDDHGDTLRWTVSVIVLILWWAFAATLRDKVVRPLQTLSNLLNALREGDF
                     SLRARQTQEMDALGEVMHEVNAIGEVLREQRLGAVEATALLHRVMEEVDVAVFAFDDQ
                     RRLQLANRAAERLLDEPAERLLGRPADELDLTELLDVDMPGTVARAFPSGPGRWDIRR
                     STFRESGLPHQLLVISDVSQTLREEERQAWKRLIRVLGHELNNSMAPIHSMAATLGRL
                     LDREPRPNDWQDDMRRGLEIIGDRSEALIRFMAAYARLARLPPPRLAPVELSALIRRV
                     AALENRLPVFVENGPRVEVQGDADQLAQLLINLVRNGVDAALETGGLVGVGWRVAGSH
                     VEVHVQDEGPGLSNTENLFVPFYTTKPGGTGIGLALSRQIAEAHGGTLTLENRAPDPG
                     CEAKLRLPL"
     gene            complement(1467..2837)
                     /locus_tag="VEL74_01850"
     CDS             complement(1467..2837)
                     /locus_tag="VEL74_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYG61298.1"
                     /translation="MNSPSPGPRVLIADDQREVREALRLLLKGEGFATEEAASPAGIL
                     QAVTTRDFDVVLMDLNYTRDTTSGREGLDLLARLRALDDALPVVVMTAWGSVGLAVEV
                     MRQGARDFIEKPWDNDRLLSILHTQVSLGRALRRGERLEAENELLRANGHFTLIAQSP
                     EMRPVIELVERVGPSEANVLVTGENGTGKGVVARALHAASPRAGVPMVTVNTGGLSEA
                     VFESELFGHVRGAFTDAKTDRVGRFELADRGTIFLDEIANISLSQQAKLLRVLETGEF
                     ERLGSSKTRRADVRVISATNADLREEVAAGRFRQDLLFRLNTVEIHVPPLRARRADIF
                     PLAEYFLEQHARRYRKEVRGFEPAAAEALLAHPWPGNVRELDHAVERGVLMARGERIR
                     AQDLGLLQGAQSGGAPIEDLPLEEVERVLIQKALARYGGNVSQAAQALGLSRSALYRR
                     LEKHGL"
     gene            complement(2905..3651)
                     /locus_tag="VEL74_01855"
     CDS             complement(2905..3651)
                     /locus_tag="VEL74_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG61299.1"
                     /translation="MNASNDALIHLDGVTKVFLTDEVETHALSGIHLEIRNGEYISIA
                     GPSGCGKTTLLSILGLLDTPSDGKYWLNGKQVADLSFSERARIRNREIGFIFQAFNLI
                     GDLTVYENVELPLTYRGMPSKERKERVQEALERVGMSHRMKHYPSQLSGGQQQRVAVA
                     RAVVGKPSILLADEPTGNLDSRNSEAVMELLAELHKDGATICMVTHDPRFARYAGRTI
                     HLFDGRVVEEEQGAQREAEERELEQRGFVV"
     gene            complement(3743..>4628)
                     /locus_tag="VEL74_01860"
     CDS             complement(3743..>4628)
                     /locus_tag="VEL74_01860"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HYG61300.1"
                     /translation="AEADYQNLRAQLSSSLLSQQAQVSAAESQAEEAKLQSEADTVLA
                     KDGLIPELDLKLSKLRATQTSKQASIERERFIQGRSANQAQLAAQAARVEQARAGYEV
                     QRRRVEALRVRAGISGVLQELPVQVGQRVTPGTNLARVARPEHLKAELRIPEVQAKDV
                     IRGLRAFVDTRNGVVPGHVERVAPSVIEGSVTVDVALDGPLPQGARPDLSVDGVIQIE
                     KLENVLFVGRPAYGQPESKIDMFKLNADETEAERVQVTLGRGSVNHIEIRGGLQAGDK
                     VILSDVSQWDEQNKLRVK"
BASE COUNT          626 a         1634 c         1643 g          725 t
ORIGIN      
        1 ggcttgccag caaggacttc ggaatccgag tggatatggt catgccagtc catgatgagg
       61 ctaggctacc atcgtttcag ggtatcttgc cgccccgtca caacggcagc cgcagcttcg
      121 cctcgcagcc cgggtccggc gcccggttct ccagggtgag ggtgccgccg tgggcctcgg
      181 cgatctgccg cgagagggcg aggccgatgc ccgtccctcc cggcttggtg gtgtagaagg
      241 ggacgaagag gttctcggtg ttcgacaggc ccggcccctc gtcctgcacg tggacctcga
      301 cgtgcgagcc ggcgacccgc cagcccactc cgaccaggcc gccggtctcc agggcggcgt
      361 cgacgccgtt gcgcaccagg ttgatgagga gctgcgcgag ctggtccgcg tccccctgga
      421 cctcgaccct ggggccgttc tcgacgaaca ccggcagccg gttctccagc gcggccacgc
      481 ggcggatcag ggcggacagc tccaccggcg ccaggcgcgg cggggggagg cgggcgagcc
      541 gcgcgtaggc ggccatgaag cggatcagcg cctccgaacg gtcgccgatg atctccaggc
      601 cgcgccgcat gtcgtcctgc cagtcgttgg ggcgcggctc gcggtcgagc agccggccca
      661 gcgtcgccgc catcgaatgg atgggcgcca tcgagttgtt caactcgtga cccagcaccc
      721 ggatcagccg cttccaggcc tggcgctcct cttcgcgcag ggtctggctg acgtccgaga
      781 tcaccaggag ctggtggggg aggccgctct cgcggaaggt gctgcgccgg atgtcccagc
      841 ggccggggcc ggacgggaag gcgcgggcca cggtgccggg catgtccacg tccagcagct
      901 cggtgaggtc gagctcgtcc gccggccgcc cgagcagccg ctcggccggc tcgtccagga
      961 gcctctcggc ggcgcggttg gcgagctgca gccgccgctg gtcgtcgaag gcgaacaccg
     1021 ccacgtccac ctcttccatg acgcggtgga ggagcgcggt ggcctcgacc gcccccagcc
     1081 gctgctcgcg cagcacctcg ccgatggcgt tgacctcgtg catcacctcg cccagggcgt
     1141 ccatctcctg ggtctggcgg gcgcgcagcg agaagtcccc ctcgcgcagg gcgttgagca
     1201 ggttcgacag cgtctggagc gggcggacca ccttgtcgcg cagggtggcg gcgaaggccc
     1261 accagaggat cagcacgatc accgacaccg tccagcgcag ggtatcgccg tggtcgtccc
     1321 accagaggag cagcagggcg atcgagaccc ccggcaggcc ggtgatcagg gcccacagca
     1381 gcacccgccg ctcgaacggc atcgccggct tcggggcgcc gcgctccccg gcctcctcca
     1441 gatgccgctc cagcgtctcc cggacgctag aggccatgct tctcgaggcg ccggtagagg
     1501 gcgctgcggg agagtccgag ggcctgggcg gcctggctga cgtttcctcc atagcgggcc
     1561 aacgctttct ggatcagcac ccgttccact tcttcgagcg gcaggtcctc gatcggcgcg
     1621 ccgccgctct gcgctccctg gagcagcccc aggtcctgcg cccggatccg ctccccccgc
     1681 gccatcagca cgccgcgctc caccgcatgg tcgagctcgc ggacgttgcc cggccagggg
     1741 tgggcgagca gcgcctccgc cgccgccggc tcgaaccccc gcacctcttt acgataacgg
     1801 cgggcgtgct gttccaggaa atactcggcc agcgggaaga tgtcggcgcg gcgggcgcgc
     1861 agcggcggca cgtggatctc caccgtgttc agccggaaca gcaggtcctg ccggaagcgg
     1921 ccggcggcca cctcctcgcg caggtcggcg ttggtcgccg agatcacccg cacgtcggcg
     1981 cgccgggtct tcgacgaccc cagccgctcg aactcgccgg tctccagcac ccgcagcagc
     2041 ttcgcctgct gggagaggga gatgttggcg atctcgtcga ggaagatggt cccccggtcc
     2101 gccagctcga agcgccccac ccggtcggtc ttggcgtcgg tgaaggcgcc gcggacgtgg
     2161 ccgaagagct cgctctcgaa gaccgcctcc gacaggccgc cggtgttcac cgtcaccatc
     2221 gggaccccgg cgcgcggcga cgcggcgtgg agggcgcgcg ccaccacccc cttgccggtg
     2281 ccgttctcgc cggtgaccag gacgttggcc tccgacgggc cgacccgctc gacgagctcg
     2341 atcaccggcc gcatctcggg cgactgggcg atcagggtga agtggccgtt ggcgcgcagc
     2401 agctcgttct cggcctcgag ccgctcgccg cgccgcagcg cccggccgag gctcacctgg
     2461 gtgtgcagga tggagagcag ccggtcgttg tcccacggct tctcgatgaa gtcgcgcgcc
     2521 ccctggcgca tcacctcgac cgccaggccg acgctccccc aggcggtcat caccaccacc
     2581 ggcagcgcgt cgtcgagcgc gcgcaggcgg gcgaggaggt cgagcccctc ccgccccgag
     2641 gtggtgtcgc gagtgtagtt caggtccatc agcaccacgt cgaagtcgcg ggtggtgacc
     2701 gcctggagga tcccggccgg cgaggcggcc tcctcggtgg cgaagccttc ccccttgagc
     2761 agcaggcgca gcgcctcgcg cacctcgcgc tggtcgtcgg cgatgaggac ccgggggccg
     2821 ggagaggggc tgttcatggg gaagaaagca aaagctgccg cccggctggg cggcagctcc
     2881 atggtacgtc agaacggggc ggggttatac gacgaatccc cgctgctcca gctcgcgctc
     2941 ctcggcctcg cgctgggcgc cctgctcctc ctcgacgacg cggccgtcga acaggtggat
     3001 ggtgcggccg gcgtagcggg cgaagcgcgg gtcgtgggtg accatgcaga tggtcgcgcc
     3061 gtccttgtgc agctcggcga gcagctccat gaccgcctcg ctgttgcgcg agtcgaggtt
     3121 accggtcggc tcgtcggcga gcaggatgga gggcttgccg accaccgcgc gggcgacggc
     3181 gacgcgctgc tgctgaccgc cggagagctg cgacgggtag tgcttcatgc ggtgcgacat
     3241 gccgacccgc tcgagcgcct cctgcacccg ctccttgcgc tccttggagg gcatgccgcg
     3301 gtaggtcagg gggagctcga cgttctcgta gaccgtcagg tcgccgatca ggttgaacgc
     3361 ctggaagatg aagccgatct cacggttgcg gatgcgcgcc cgctccgaga aggagaggtc
     3421 ggccacctgc ttgccgttca gccagtactt gccgtccgat ggcgtgtcga gcaggccgag
     3481 gatcgacagc agggtggtct tgccacagcc ggacgggccg gcgatggaga tgtactcgcc
     3541 gttccggatc tccaggtgga tccccgagag ggcgtgggtc tcgacttcgt cggtcaggaa
     3601 gaccttggtg actccgtcga ggtggatcaa ggcgtcgttg gaagcgttca tcgggtttct
     3661 ccccccttag tttcttcagc cgtcgctctc agcgttttcg ctctcagcaa tacggcggcg
     3721 atgtcgtcga ggtttctcgg gctcacttca cacgtagctt gttctgctcg tcccactgcg
     3781 agacgtccga caggatgacc ttgtcgcccg cctggagccc gccgcggatc tcgatgtggt
     3841 tgaccgagcc gcggcccagc gtcacctgca cgcgctccgc ctcggtctcg tccgcgttga
     3901 gcttgaacat gtcgatcttg ctctcgggct ggccgtacgc cggccggccg acgaagagga
     3961 cgttctccag cttttcgatc tggatgacgc cgtcgaccga caggtcggga cgggcgccct
     4021 ggggcagcgg gccatccagc gccacgtcca ccgtgaccga cccctctatg accgacggcg
     4081 cgacacgttc cacgtggccc ggcacgacgc cgttgcgggt gtcgacgaac gcccgcaggc
     4141 cgcggatgac gtccttggcc tgcacctccg ggatgcgcag ctcggccttg aggtgctcgg
     4201 ggcgggccac ccgggcgagg ttggtgcccg gcgtcacccg ctggccgacc tgcaccggca
     4261 gctcctggag cacgccggag atgccggcgc gcacgcggag cgcttcgacg cggcggcgct
     4321 gcacctcgta gccggcgcgc gcctgctcga cgcgggcggc ttgggcggcg agctgggcct
     4381 ggttggcgct gcgcccctgg atgaagcgct cgcgctcgat gctggcctgc ttgctggtct
     4441 gcgtcgcgcg cagcttcgac agcttgaggt cgagctcggg gatcaggccg tccttggcca
     4501 gcaccgtgtc ggcctccgac tggagcttgg cctcctcggc ctgcgactcc gccgccgaca
     4561 cctgggcctg ctggctcagc agcgagctcg agagctgggc acggaggttc tggtagtcgg
     4621 cctcggcg
//
LOCUS       DASPJI010000070         3865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_236103, whole genome shotgun sequence.
ACCESSION   DASPJI010000070 DASPJI010000000
VERSION     DASPJI010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236103"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="VEL74_01865"
     CDS             complement(<1..1539)
                     /locus_tag="VEL74_01865"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HYG61301.1"
                     /translation="MKYRSIPLTALGLLAALPFLAAANDQPPKAPSKVEPPASQVVMP
                     TSMPTLSVDQVKRGQRGYGLSVFAGTEPERFEVEVIWVMRNVSPNVSYILARLEGKGL
                     DKIGVAAGMSGSPVFIDGKLVGAVSFSWPFSQETIGGITPIEDMRRLSGLGPLPATPL
                     PPVPLADIMARKIPADLLERELARLQPRLAGGGLPGIVWATQGFGDQSLAMMRRALQT
                     VSAAGEARPEDVPRDLEMGGAVAAVLVDGDFGLAGFGTVTDRYEDQVLAFGHPFMGLG
                     PVQVPMATAEVVTIFSSQLASFKISNIGHTIGAFEQDRQAGIQGRIGATAPMTPMVLR
                     VAGGLGEKPREYKVRVAEIPLLTPSLIGSTVVAGLESASYTAGSQSLDMTARFKLARY
                     GDLLVRQSFDGASAGSEAAAYVLAVAGFLTQNDFEKVDLEAVEVDIQQSPEPRAATLV
                     GAHAERTVVRPGERIGLNLDLMAYRGERFRRSLNIDLPQDLPAGRYTLLVGDGSSADA
                     ARMTL"
     gene            complement(1536..2180)
                     /locus_tag="VEL74_01870"
     CDS             complement(1536..2180)
                     /locus_tag="VEL74_01870"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HYG61302.1"
                     /translation="MTPAPLWHWKEPVAPLAALLARGGVLAIPTESSYGLAAHPASAA
                     GVQTIYKVKARDVGKPLPVVVAEMGQLTGLGIPPDLPILERLSACWPGPLSVVLPLRW
                     PLPAAAGGTGLAVRIPGHAELRRLLAALGHGLTATSANLSGGEPILDPAEAAALLAGE
                     DAVVVDGGILPGGPPSTLVAIEETGPVVLRTGSFPVERLRELLGSTSVHGRRTS"
     gene            complement(2177..>3865)
                     /gene="recN"
                     /locus_tag="VEL74_01875"
     CDS             complement(2177..>3865)
                     /gene="recN"
                     /locus_tag="VEL74_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HYG61303.1"
                     /translation="ETGAGKSIVVDSLALLAGARASAEMIRSDAESLTVSGVFEPGGS
                     AWRTILEEAGLEAGEEMGGEVLVRREIARSGRNRVFVNDQPTTLRLLADLAPYLLRIH
                     GQRDELGLIEPDLQRAMLDRSGGLEAAPLLGRVAAAFDQYIQLAERLERLSGDDRVRR
                     ERIDLLRFHAAEIDAAKIRAGEDVELRSEREVLRHAEAITRSLGTCFSLLSEEEGSAL
                     ERIGKSQSLLADVEEWEPQAAGWGTELESVRIQLAELAAALSHRLDGLEADPARLDAV
                     EDRLAVLERICKKHGGDTAAVLARRQEMEAELAELEGDAENRDELTGRVAAALADYRQ
                     AALALSAARAAWGKALVERIEGELKDLGLARARLAVALERRRRAGSALVLSGDGGDEP
                     VDFGREGVDQVVFLFAPNPGEEPRPLARIASGGELSRIHLALQLAAHGETEEARPTLV
                     FDEVDTGIGGAEAAALGKKLQRLAHGGQILAVTHLPQVASHADLHFRVSKHVDGGRTR
                     VRVETLEEGARVEEVARMLAGQEVTQLSLSHAQELITGAAREREKGRARRRAAR"
BASE COUNT          542 a         1429 c         1373 g          521 t
ORIGIN      
        1 cagcgtcatc cgcgccgcgt cggcgctcga gccgtccccc accagcagcg tatagcggcc
       61 ggcgggcagg tcctgcggca ggtcgatgtt cagcgaccgg cggaagcgct cgccgcggta
      121 cgccatcagg tcgaggttca ggccgatgcg ctcgccgggc ctcaccacgg tgcgctccgc
      181 gtgcgccccc accagcgtcg cggcgcgcgg ctcgggcgac tgctggatgt cgacctccac
      241 cgcctcgaga tccaccttct cgaagtcatt ctgggtcagg aacccggcca ccgccagtac
      301 ataggcggcg gcctcgctgc cggcgctcgc gccgtcgaag ctctgccgta ccaggaggtc
      361 accgtagcgc gccagcttga agcgggctgt catgtccagg ctctgtgaac cagccgtgta
      421 gctcgccgac tcgagccccg ccaccaccgt gctgccgatg agcgacgggg tgagcagcgg
      481 gatctcggcg acgcgcacct tgtactcccg cggcttctcg cccaggccgc cggcgacgcg
      541 cagcaccatg ggggtcatcg gcgcggtggc gccgatccgc ccctggatgc cggcctggcg
      601 gtcctgctcg aaggcgccga tggtgtggcc gatgttggag atcttgaacg aggcgagctg
      661 gctggagaag atcgtcacca cctcggcggt cgccatcggc acctggaccg gcccgagccc
      721 catgaagggg tgaccgaagg cgagcacctg gtcctcgtag cggtcggtga cggtgccgaa
      781 gcccgccagc ccgaaatcgc cgtccaccag caccgcggcc accgcgcctc ccatctcgag
      841 gtcccgcggc acgtcctctg gccgcgcctc gccggccgcg ctcacggtct ggagagcgcg
      901 gcgcatcatc gccagcgact ggtcgccgaa gccctgcgtc gcccagacga tccccggcag
      961 gccgccgccc gcgaggcgcg gctggagcct ggccagctcg cgctccagca ggtcggcggg
     1021 gatcttgcgc gccatgatgt ccgccagcgg caccggcggc agcggggtgg cgggcaacgg
     1081 tcccaggccg gagagccggc gcatgtcctc gatcggggta atgccgccga tagtctcctg
     1141 cgagaacggc cagctgaacg acacggcgcc caccagcttg ccgtcgatga acaccgggct
     1201 gccgctcatg ccggcggcga cgccgatctt gtccaggccc ttgccctcga ggcgggcgag
     1261 gatgtagctg acgttggggc tgacgttgcg catcacccag atcacctcga cctcgaagcg
     1321 ctcgggctcg gtgccggcga agaccgacag gccatagccg cgctgccccc gcttcacctg
     1381 gtcgacggag agggtgggca tcgaggtggg catcaccacc tgggaggcgg gaggctcgac
     1441 cttggacgga gccttggggg gctggtcatt ggcggccgca aggaaaggca gggcggcgag
     1501 caggcccaga gccgtgagag ggatcgaccg atacttcatg acgtacgtct cccgtggacc
     1561 gacgtggaac cgagcagctc gcgcagccgc tcgacgggga agctccctgt ccgtagaacc
     1621 accggcccgg tctcctctat tgccaccaaa gtcgaaggtg ggccgccggg caggatcccg
     1681 ccgtccacca ccaccgcgtc ctccccggcc agcagcgccg ccgcctcggc ggggtccagg
     1741 atgggctcgc cgccgctcaa gttggcgctc gtggcggtga ggccgtgccc aagggcggcc
     1801 aggagccgcc gcaactcggc atgtccggga atccgcaccg ccaacccggt cccgcccgcc
     1861 gcggccggca gcggccaccg caggggaagc acgacggaaa gcggccccgg ccagcaggcg
     1921 gagaggcgct ccagtatagg gagatcgggg ggaatcccca agccggtgag ctgccccatc
     1981 tccgccacca cgacagggag gggcttgccc acgtcccgcg ccttcacctt atagatggtc
     2041 tggacccccg cggcggacgc ggggtgggcg gcgaggccgt agctcgattc ggtgggaatg
     2101 gccaggacac cgccccgggc gagcagggcc gcgagcggcg ccaccggctc cttccaatgc
     2161 cagagagggg ccggggtcat cgcgccgccc gccgccgggc gcgccccttt tcccgctcgc
     2221 gagccgcgcc ggtgatcagc tcctgggcgt gggagaggga gagctgggtg acctcctggc
     2281 cggccagcat gcgggcgacc tcctccaccc gggccccctc ctccagcgtc tcgacccgca
     2341 cgcgggtgcg cccaccgtcc acgtgtttgc tcaccctgaa gtggaggtcg gcgtgcgagg
     2401 cgacctgggg caggtgggtc accgccagga tctggccacc atgggccaag cgctggagct
     2461 tcttgcccag ggccgccgct tcggcgccgc cgatgccggt gtccacctcg tcgaagacca
     2521 gcgtcggccg cgcctcctcg gtctcgccgt gcgccgcgag ctggagggcg aggtggatgc
     2581 gcgacagctc gccgcccgag gcgatacggg cgagcggccg cggctcctcg ccgggattgg
     2641 gggcgaacag gaacaccacc tggtcgaccc cctcgcggcc gaagtccacc ggctcgtcgc
     2701 ctccgtctcc cgagaggacg agcgcgctcc ccgcgcgcct ccggcgttcg agggcgacgg
     2761 cgaggcgggc gcgggcgaga cccaggtcct tgagctcccc ttcgatacgc tcgaccagcg
     2821 ccttgcccca agcggcgcgg gccgcggaga gggccagggc ggcctgcctg tagtcggcca
     2881 gggccgccgc cacgcgcccg gtgagctcgt cccggttctc cgcgtcccct tccagctcgg
     2941 cgagctcggc ctccatctcc tggcgccggg ctagcaccgc ggcggtgtcc ccgccgtgct
     3001 tcttgcagat ccgctcgagg acggcgaggc ggtcctccac cgcgtcgagg cgggccgggt
     3061 cggcttccag gccgtcgagc cggtgggaga gggcggcggc gagctcggcg agctggatgc
     3121 gcaccgactc cagctccgtc ccccagccgg cggcctgcgg ctcccactcc tcgacgtcgg
     3181 ccagcaggct ctggctcttg ccgatcctct ccagggccga gccctcctcc tcggagagga
     3241 gggagaagca agtgcccagg gagcgggtga tcgcttcggc gtggcgcagc acctcgcgct
     3301 ccgagcgcag ctccacgtct tcgccggcac ggatcttggc ggcgtcgatt tcggcggcgt
     3361 ggaagcgcag cagatcgatg cgctcgcgcc gcacccggtc gtcgccggag aggcgctcga
     3421 gccgttcggc aagctgaatg tactggtcga aagccgcggc gacccggccg aggagaggcg
     3481 ccgcctcgag accgccgctg cggtcaagca tggcacgctg gaggtcgggc tcgatgagcc
     3541 ccagctcgtc gcgctggcca tggatgcgca ggaggtaagg ggcgagatcc gccagcaacc
     3601 gcagtgtcgt cggctggtcg ttgacgaaga cccggttgcg gccgctcctc gcgatctcgc
     3661 ggcgcaccag gacctcgccg cccatctcct cccccgcttc gaggcccgcc tcttcgagga
     3721 tcgtccgcca ggccgagccg cccggctcga acacgccgct caccgtcagc gactcggcat
     3781 cgctgcggat catctcggcc gaggcccggg ccccggcaag gagcgccagg gagtcgacga
     3841 cgatggactt gcccgccccc gtctc
//
LOCUS       DASPJI010000071        12810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_633479, whole genome shotgun sequence.
ACCESSION   DASPJI010000071 DASPJI010000000
VERSION     DASPJI010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12810)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12810
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633479"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VEL74_01880"
     CDS             <1..260
                     /locus_tag="VEL74_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61304.1"
                     /translation="LEFEGVKDLVRMWMTSSSEDDLGFMERMDGTQLVSSVIQALPFC
                     AAERQALMEAPSLEDIEDLLLTLVEGVGERFRQDDPLPVAH"
     gene            312..3611
                     /locus_tag="VEL74_01885"
     CDS             312..3611
                     /locus_tag="VEL74_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 lanthipeptide synthetase LanM family
                     protein"
                     /protein_id="HYG61305.1"
                     /translation="MQTPFPEAWRAFTLAERAAALRRLPRPLPPLGERERTLAEKRLA
                     RWRSQAPLDRDDVFASRLAAEGLSAEDFLWLLGTPPEALASHLPPAPWLDRLSEALVG
                     EDCEPLPWPEEALRSPTAGFLQAAEPFVRRAREHLRRGLAELAPLPFDPNQTVDRLLL
                     ADLPFTLLPMLSRTLVLELHIAGLEERLEGETPEERLGAFLRELETPEARLALFGEYP
                     VLARLLVEALDRWADAGLELLRRMAADWDAIRRAFSPDRDPGLLEHLDTRAGDPHRGG
                     RTVVIARFTSGLRIVYKPKPLSVDRHFQELLAWLNANGVKPRLQTLAVLDRGTYGWVE
                     HAAARSIASPEELRRFYRRQGGYLALLFALEAVDFHYQNLIAAGEHPILVDLESLFHA
                     RLEGPQAGAGGAEHQAARSLSYSVLRTGLLPRRRWYAAGDAEDAGAALDVSGLSAPDE
                     PLAPRPVPIPVAAGGDLRIIRKKLPLDRGGHRPAGDAGATLLAHGDELMAGFRATYRL
                     VAQHRGELTAAGGPLLPFADDEIRIILRPTRVYDAPLAESFHPELLRDALLRDRLFDR
                     LWLEAVEAPHLQRVVPAEIRALWRGDIPLFTTCPASRDVRAGEGEVQEGLLQESGLDL
                     VRARLASLGDSDLEHQLYFVRAALASLAPAANTVERGQQRLTGSDGIPGRDELIDAAA
                     AIGHRLEALAIRGAAGETGDGGATWVGLHAALDGTLFLDPLPAPLHHGLGGIAVFLAH
                     LGAATGEPRFQRLAREAWSTAEEQIRQDRAGLRSIGGFLGWGSLIVTLLHLGALWGEE
                     ERLAEVEGMVGEIAERLDEDQDLDVLGGTAGALIGLLRLPSDGRARELAVRCGDRLLG
                     LARPMSGQGLRGLGWWGRGSEALPLTGFSHGAAGIGWALLELATVLGEVEGERFRAAA
                     LGAFEYERSTFQPRLDNWPDLRYYDLEGRPVEGKELPEFFPAWCHGAAGIALARAAAL
                     RHLDLPELRNDLQTAVRLVLARGFGYNQSLCHGDLGNLDVLLVASRLPGVSGGGDLGR
                     QAGRIAGSILAGAREHGWRCGISYGAETPGLMAGLAGIGYGLLRLAEPDSIPSLLLLE
                     TPATAEALHAP"
     gene            3681..3875
                     /locus_tag="VEL74_01890"
     CDS             3681..3875
                     /locus_tag="VEL74_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015147952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mersacidin/lichenicidin family type 2
                     lantibiotic"
                     /protein_id="HYG61306.1"
                     /translation="MKKRDIIRAWRDEEFRLSLSEEQRAQLPANPAGNVELADEDLRS
                     ASGGWPSLRPVLCPSLATCG"
     gene            3980..4177
                     /locus_tag="VEL74_01895"
     CDS             3980..4177
                     /locus_tag="VEL74_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006100521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mersacidin/lichenicidin family type 2
                     lantibiotic"
                     /protein_id="HYG61307.1"
                     /translation="MKKRDIIRAWRDEEFRLSLSEEQRAQLPVNPAGSVELADEDLLS
                     VSGGWSSCRRPCFCPSETTCY"
     gene            complement(4227..5534)
                     /locus_tag="VEL74_01900"
     CDS             complement(4227..5534)
                     /locus_tag="VEL74_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HYG61308.1"
                     /translation="MKTMTDPNDPIRDPELDRLVRELNSSEALPEEAVLDGVPPTLEG
                     VLPKASERLLDIPAQAQVMEGEPLDRLLAEMVRQRASDLLLVAGLPPIFRIDGRLLRA
                     DSDVLEGDEISSMLQPHLGARARRDMEERGSADLSLRLAPGREVRTDEGPRSGGASPS
                     SSWRFRVNLHRQGGQLAAAMRALPTDIPSLAGLNLPASFAELVRPNRGLVLVCGPTGS
                     GKSSTLAALVAELNRTRSCHIITIEDPVEYEHRSAKAVVEHVEIGRDARSFPEALRAS
                     LRQDPDVILVGEMRDLETVATALTAAETGHLVLSTLHTNDAAQAVHRIVDVFPPAQQG
                     QIRHQLALSLHAIVVQQLVPRCDGVGRVPAVELMLATYAVRHHIRSDNLQKLYNEITL
                     GKRLGMISMEQSLADLVHMGRIDQDEARVRASHPDEFETLVRI"
     gene            5644..6126
                     /locus_tag="VEL74_01905"
     CDS             5644..6126
                     /locus_tag="VEL74_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HYG61309.1"
                     /translation="MPIEVGDKIPNVKLQTMTSNGPRAISMDEICRGQKVVLFAVPGA
                     FTSTCSNDHLPGYVKWVDDIKAKGVDVVACVAVNDVFVMNAWANARGVDDKILMLSDG
                     NGDFAKAMGRELDSRAFGMGVRSQRYAAVIDDGVVKALHQDAPGEVKVSGAEAILAEL
                     "
     gene            6229..6981
                     /locus_tag="VEL74_01910"
     CDS             6229..6981
                     /locus_tag="VEL74_01910"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HYG61310.1"
                     /translation="MIPDRRRALAVLNVFIALALASPREAAATWSIVAVDPETRQVGS
                     AGASCTPFVAGIVAVAPGKGVIVAQAMSNAEARLHGVGMLAKGASPAQVIAAVANKEF
                     DSDWQEQQYGVAALGFEDASAAFTGAGTYDVKGDLQGYGVSVQGNILTNKEVIERALA
                     AFLAPPAKPATSRSLAERLLSALEAGAEAGGDKRCGKQTAMSSYVVVAKPDDDPVHPS
                     TLVIVREDEGGANPVRVLRERYERARQKRQGH"
     gene            7022..7228
                     /locus_tag="VEL74_01915"
     CDS             7022..7228
                     /locus_tag="VEL74_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61311.1"
                     /translation="MHLHLRVDENAFLTAARRIAEEQSVFTWPRTTPGATPSVRVVEF
                     TAGDATLGLTAEEVAGIVEQLVAG"
     gene            complement(7859..9775)
                     /locus_tag="VEL74_01920"
     CDS             complement(7859..9775)
                     /locus_tag="VEL74_01920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYG61312.1"
                     /translation="METLHPDHQSQSARTGPLFSWLLAVLLLLSFLLRLWLACQGLHT
                     GHYGDERFAFRNVTGLLQGEIRPRHAFYPSLSYLPQSAVLAASWGLHELTGIEALSIF
                     SEKAVDGYSPTAYLLCRLCNVLFGVLALWMVFLIGRRIHSPAAGLLAAAVLAALHRHV
                     LSSTQFKPDILVLFLTTVTFYWTLAAAFRPALGRYARVGAGIGLAVSAKYTGIASCLP
                     ITAAALCNGLGPRRDRCQLGWLVAAGLISIATFVVLNPFLGVVLHYIPLQASSYAARV
                     ENEPSGHWVVFIRQIEFLIVHHGWLTALFVLAGTVGLLWRYRRPVPGDGGERRVGWLL
                     VLSLFLGYSALHTAGMTLFRSQNYLPVAPFTSLIAAWAMVEAWRALVHRVPALSRRPA
                     AATAVVAAGLVLLADQAAVVYKWVIPNTWAEARHVLTARLAPIGLRHVVYEKGTNILR
                     LGGKNRALITAAGRLGELGPAFLERADAEVFPDSRLHGPDADFYRRRLERLPPDQVVR
                     VSSRPFHSRGETLVLLLHPWRQDGRFELPLGRPPQVRRRQTSVLPPGIAPGDVLSAVL
                     WAPREAQRLEGLRLDPGGELVRTFEAGSRARKVRLMTRRFALTGGETRVRLVSPPGRY
                     PEGYRLELYRWRPR"
     gene            9990..10421
                     /locus_tag="VEL74_01925"
     CDS             9990..10421
                     /locus_tag="VEL74_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61313.1"
                     /translation="MLRLTRVIAFALLSTQVYSYDALNRLASVSQPWTGAGSTEAVTT
                     YGYDLQAHLTSVTDAEGNITTYTYSDRDLLTQQTSPVSGVTTHQYDDHGELIAETDAR
                     GITVEREVDILGRVVLVDYPDEALDTTYSYDDPAVPFSKAD"
     gene            10521..10964
                     /locus_tag="VEL74_01930"
     CDS             10521..10964
                     /locus_tag="VEL74_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61314.1"
                     /translation="MNHKTAEHIISVSMSCINRLDRSVEDAIDAAPEEHASSYRSLAG
                     EVMGYLFTEVLMPIYAAHPDLEPEELREARVSSQPTSMPPEIAAKLMNVCTSVSQSLR
                     DLCEEVALSPRETDFELESALQEPLKALRHTQEFLRRACPDLEPG"
     gene            complement(11001..11444)
                     /locus_tag="VEL74_01935"
     CDS             complement(11001..11444)
                     /locus_tag="VEL74_01935"
                     /inference="COORDINATES: protein motif:HMM:NF017482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HYG61315.1"
                     /translation="MARKGPPPPPDPENGLWQGRSVARRFVSPDGMTVLVGRTAEDND
                     VLTFKLGAPRDFWMHIAGDSGSHVVVRNPEGLERLPKETERFAASLAAGYSKARNGGR
                     VAVHVCTCADVRKPRGLPAGKVVLERFKNLQVAPARGEGGDGASV"
     gene            complement(11444..11791)
                     /locus_tag="VEL74_01940"
     CDS             complement(11444..11791)
                     /locus_tag="VEL74_01940"
                     /inference="COORDINATES: protein motif:HMM:NF017840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HYG61316.1"
                     /translation="MQVMHFYRDEAGDPRANSPEPQELLARFLESDIQGSEDYARDIL
                     GAIERVEAGKEPSWQETGNAHTLILDSAGATIEAEYEEDGEAPPCRVSLDDLRAALIG
                     WLAFLERTSREDR"
     gene            complement(11806..12297)
                     /locus_tag="VEL74_01945"
     CDS             complement(11806..12297)
                     /locus_tag="VEL74_01945"
                     /inference="COORDINATES: protein motif:HMM:NF025792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HYG61317.1"
                     /translation="MRRPQRSSALVILVCAVLAALVGLGALQAAARWTATKPRINAAH
                     VFEGEINRKGRPVGFHSRPGGRDPRGARVSQVVSRPNRQGVYEARVEIRDPESGRWLG
                     KRSTMFPDSLSREEVVRAILNAYRQRTTGKAERFRGPSGRGFTIEGYLLRGDINTAYP
                     IYR"
     gene            complement(12323..12691)
                     /locus_tag="VEL74_01950"
     CDS             complement(12323..12691)
                     /locus_tag="VEL74_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61318.1"
                     /translation="MTRATRYLLTTDAKGQLVKVEQMGEAGDLSEVDLRWFVSQLAPP
                     PAAAQQDVHIHLHVHGPGGHDGGHGPGPRVVIPGPGSPWTPLIGPGGPGAPGAPGAQL
                     SGPGGPGGPGAPGGPGTTGE"
BASE COUNT         1989 a         4470 c         4445 g         1906 t
ORIGIN      
        1 gactggagtt cgagggggtc aaggacctcg tccgcatgtg gatgacgagc agctccgagg
       61 acgacctcgg cttcatggag cgcatggacg gcacccagct cgtctcctcg gtcatccagg
      121 ccctcccctt ctgcgccgcg gaacggcagg cgctgatgga ggcccccagc ctggaggaca
      181 tcgaagacct actcctcacc ctcgtggaag gggtgggcga gcgcttccgc caggacgacc
      241 cgctcccggt cgcccactga ccggcggcgc ccgggcttcg cccagtccgc ctctgctatc
      301 ttcgtcgcgc catgcagacg ccttttccag aagcctggag ggccttcacc ctggccgagc
      361 gcgccgcggc gctccgccgc cttccccgtc ccctgccccc tctcggcgag agggagcgga
      421 cgctggcgga gaagcggctc gcgcgctggc gctcccaggc cccgctcgac cgcgatgatg
      481 tcttcgccag ccgactcgcc gccgaggggc tgtccgcgga ggacttcctc tggctcctgg
      541 gcactccgcc cgaggccctc gcgtcccatc tccctcccgc tccgtggctg gacaggctgt
      601 ctgaggccct cgttggagag gactgcgagc cgctgccgtg gccggaggag gcgctccgct
      661 cgcctacggc cgggttcctc caggccgccg agccgttcgt ccgccgggcg cgggagcacc
      721 tgcggcgggg gctcgcggag ctggcccccc tgcccttcga tccgaatcag accgtggacc
      781 ggctgctgct ggccgacctc cctttcaccc tgctgccgat gctcagccgt accctcgtgc
      841 tggagctcca catcgccgga ctggaggagc ggctggaggg ggagacgccc gaggagcgcc
      901 tcggcgcctt cctgcgtgag ctggagacgc cggaggcacg gctcgcgttg ttcggcgagt
      961 acccggtcct ggcacggctg ctggtggaag ctctggaccg ctgggccgac gcggggctcg
     1021 agctgctccg ccgcatggcc gcggattggg acgccatccg ccgcgccttc tcgccagacc
     1081 gcgacccggg cctgctggag cacctggaca cccgcgccgg cgacccccac cgcggcggcc
     1141 ggacggtggt catcgcccgc ttcacctcgg gcctgcgcat cgtctacaag cccaagcccc
     1201 tgagcgtgga ccgccacttc caggagctcc tcgcctggct caatgccaac ggcgtcaagc
     1261 cgcggctgca gacgctcgcc gtcctcgacc gcggcaccta cggctgggtg gagcacgccg
     1321 cagcccgctc catcgcctcg cccgaggagc tccgccgctt ctaccgccgg cagggcggct
     1381 acctggcgct gctcttcgcc ctggaggccg tcgacttcca ctatcagaac ctgatcgcgg
     1441 cgggcgagca cccgatcctc gtagacctcg agtcgctctt ccatgcccgc ctcgagggcc
     1501 cccaggcagg ggccggcggc gccgagcacc aggcggcccg cagcctctcc tactcggtgc
     1561 tccgcaccgg cctgctcccc cggcggcgct ggtacgcggc cggcgacgcc gaggatgccg
     1621 gggcggcgct cgacgtcagc ggcctctcgg cgcccgacga accgctggcg ccgcggcccg
     1681 tgccgatccc cgtcgcggcc gggggcgacc tgcgcatcat ccgcaagaag ctgcccctgg
     1741 accgcggcgg tcaccgtccg gcgggcgatg ccggggctac cctgctggcc cacggcgacg
     1801 agctgatggc gggcttccgt gccacctacc gcctggtcgc ccaacaccgc ggcgagctca
     1861 ccgcggccgg cggccccctc ctccccttcg cggacgacga gatccggatc atcctccgcc
     1921 cgacccgggt ctacgacgcg ccgctcgccg agagcttcca tcccgagctg ctgcgcgacg
     1981 ccctgctccg cgaccgcctg ttcgaccggc tgtggctcga ggccgtggag gccccccacc
     2041 tccagcgcgt cgtgccggcg gagatccgcg ccctgtggcg gggagacatc ccgctgttca
     2101 ccacctgccc cgcctcgcgg gacgtccgcg ccggcgaagg agaggtccag gaagggctgc
     2161 tccaggagtc cggcctcgac ctggtgcgcg cccgtctcgc gagcctcggc gatagcgacc
     2221 tcgagcacca gctctacttc gtccgcgcgg ccctggcgag cctcgcgccg gcggcgaaca
     2281 cggtggaacg cggccagcag cggctcaccg gcagcgacgg gatccccggc cgggacgagc
     2341 tgatcgatgc cgccgcggcg atcgggcacc ggctggaggc gctggcgatc cgcggcgccg
     2401 ccggagagac gggtgacggc ggcgccacct gggtcgggct ccacgccgct ctggacggca
     2461 cgctgttcct cgatcctctc cccgcgccgc tgcatcacgg gctcgggggc attgccgtct
     2521 tcctcgccca tctcggcgcc gccaccggcg agccgcgctt ccagcgcctg gcgcgcgagg
     2581 cctggagcac cgccgaggag cagatccggc aggaccgcgc cggcctccgt tcgatcggcg
     2641 gcttcctggg ctggggctcg ctgatcgtca ccctgctcca cctgggcgcc ctctggggcg
     2701 aggaggagcg cctggccgag gtcgagggca tggtgggcga gatcgcggaa cgccttgacg
     2761 aggaccagga cctcgacgtc ctcggcggca cggcgggggc attgataggg ctgctgcggc
     2821 tgccgtccga cggccgggcg cgggagctgg cggtccgttg cggcgaccgc ctgctcggcc
     2881 tcgcccggcc gatgagcggt caggggctgc gcggcctcgg ctggtggggc cgcggctccg
     2941 aagcgctccc cctcaccggc ttctcgcatg gcgccgccgg gatcggctgg gcgctcctgg
     3001 agctcgccac ggtgctcggg gaggtggagg gcgagcggtt ccgcgccgcc gccctgggtg
     3061 ccttcgagta cgagcgctcc accttccagc cgcgcctcga caactggccc gacctccgct
     3121 attacgacct cgaaggccgg ccggtggaag gcaaggagct gcccgagttc ttccccgctt
     3181 ggtgccacgg cgccgccggc atcgcgctcg cccgcgcggc ggccctgcgc cacctcgacc
     3241 tgccagagct gcggaacgac ctccagaccg ccgttcgcct ggtgctggcg cggggttttg
     3301 gttacaacca gagtctctgc catggcgacc tcggcaatct ggacgtgctg ctcgtggcga
     3361 gccgcctgcc cggtgtttcc ggcggtggcg acctgggtcg gcaggcgggt cgcatcgctg
     3421 gctcgatcct cgccggcgcg cgcgagcacg gctggcgctg cggcatctcc tacggcgccg
     3481 aaacgccggg cctgatggcg ggcctcgcgg gcatcggcta cggcctgctc cgcctcgccg
     3541 aaccggattc catcccctcc ctcctcctgc tcgagacccc ggctacggcg gaggcgttgc
     3601 acgcaccata aaacgatgag cgggcaaccc ggcgagcggg tgcgtccgtc aacggcaaca
     3661 ccgacccagg ggggtgggac atgaagaaga gagacattat tcgcgcctgg agagacgaag
     3721 agttccggct gagcctcagc gaggagcagc gtgcccagtt gccggccaac ccggcaggca
     3781 acgtcgagct ggcggacgag gatctgcgct ccgccagcgg cggctggccc agccttcgcc
     3841 ccgtcctttg ccccagcctc gcgacctgcg ggtaaagacg caggctcgat ccgacgtgtt
     3901 gcgcgcacca tgaactacga gcgggccgtc cggcgtgcgg gcgcgtccgt tcaccgcact
     3961 ttgacaaggg aggtgggata tgaaaaagag agacatcatc cgcgcctgga gagacgaaga
     4021 gttccggctg agcctgagcg aggagcagcg cgcccagttg ccggtcaacc cggcgggtag
     4081 cgtcgagctg gcggacgagg acctgctctc ggtcagcggc ggctggtcga gctgccgccg
     4141 cccctgcttc tgccccagcg agaccacctg ctactagagc cggcctccgc ggagggggga
     4201 gcgctccccc ctccgacctc cacccgtcaa atccgcacca acgtctcgaa ctcgtcagga
     4261 tggctggcgc gcacccgcgc ctcgtcctgg tcgatcctcc ccatatggac caggtcggcg
     4321 agcgactgct ccatggagat catccccagg cgcttgccca gcgtgatctc gttgtagagc
     4381 ttctgcaggt tgtcgctgcg gatgtggtgg cgcaccgcgt aggtggcgag catcagctcc
     4441 acggcgggga cgcggccgac gccgtcgcag cgcggcacga gctgctggac gacgatggcg
     4501 tgcagcgaca gggcgagctg atgccggatc tgcccctgct gggccggggg gaagacgtcg
     4561 acgatccggt gcaccgcctg ggcggcgtcg ttggtgtgca gggtggagag caccaggtgg
     4621 ccggtttcgg ccgcggtgag cgcggtcgcg accgtctcca ggtcgcgcat ctcccccacc
     4681 aggatcacgt ccgggtcctg gcgcagggag gcccgcagcg cttcggggaa ggagcgcgcg
     4741 tcccggccga tctccacgtg ctcgaccacg gccttggcgg agcggtgctc gtactccacc
     4801 gggtcctcga tggtgatgat gtggcaggag cgggtgcggt tgagctccgc caccagcgcc
     4861 gccagggtcg acgacttgcc cgagcccgtg gggccgcaga ccaggaccag gccgcggttg
     4921 gggcggacca gctcggcgaa gctcgcgggc aggttgagcc ccgcgaggct cgggatgtcc
     4981 gtcggcagtg cgcgcatcgc cgcggcgagc tgccccccct ggcgatggag gttgacgcgg
     5041 aagcgccacg aggaggacgg cgaggcgccg ccggagcgcg gcccctcgtc ggtgcgcacc
     5101 tcgcggccgg gcgccaggcg gagcgagagg tcggcggacc cgcgctcctc catgtcccgg
     5161 cgggcgcggg cgcccaggtg cggctggagc atggaggaga tctcgtcgcc ctccaggacg
     5221 tccgagtccg cgcggaggag gcgcccgtcg atccggaaga tggggggcag cccggccacc
     5281 agcagcaggt cgctcgcccg ctggcggacc atctcggcga gaagccggtc caggggctcc
     5341 ccttccatga cctgggcctg ggcggggatg tcgagcaggc gctccgacgc tttcgggagg
     5401 acgccttcca gcgtcggcgg gacgccgtcc agcaccgcct cctcgggcag cgcctcgcta
     5461 ctgttcagct cgcggaccag acggtcgagc tcggggtcac ggatggggtc gttcgggtcg
     5521 gtcatcgtct tcatcggcct ctcctaagca tacggatcag aggccctccg cgttgagaag
     5581 gtatacgatc tcaggtcgcc ggcgccctcc gcgagggtcc ggccaaccag agaggggaga
     5641 cctatgccga tcgaggtcgg agacaagata ccgaacgtga agctgcagac gatgacgtcc
     5701 aacgggcccc gggcgatctc gatggacgag atctgtcgcg gccagaaggt ggtgctgttc
     5761 gccgttcccg gcgccttcac ctccacctgc tccaacgacc atctgcccgg ctatgtgaag
     5821 tgggtggacg acatcaaggc caagggggtg gacgtggtcg cctgcgtggc ggtcaacgac
     5881 gtcttcgtga tgaacgcctg ggcgaacgcc cggggcgtcg acgacaagat cctcatgctc
     5941 tccgacggca acggcgactt cgccaaggcc atgggccgcg agctcgactc ccgcgccttc
     6001 ggcatggggg tccgctccca gcgctacgcc gccgtcatcg acgacggcgt ggtcaaggcg
     6061 ctgcaccagg acgctccggg cgaggtgaag gtgagcggcg ccgaggcgat cctcgccgag
     6121 ctgtaagccg agctgccgag ctgtaagccg agctgtaaag ccgagctgtg aaaagagacg
     6181 ggcgcccggc tacactcagt cgactgttcg atcgcgagga ggttgacctt gatccctgac
     6241 cgtcgccggg cgctcgccgt cctgaacgta ttcatcgccc ttgccctggc ctcccccagg
     6301 gaggccgcgg ctacctggtc catcgtcgcc gtcgatcccg agacgcgcca ggtcggcagc
     6361 gccggcgcgt cgtgcacccc gttcgtggcg gggatcgtcg cggtggctcc cggcaagggg
     6421 gtcatcgtgg cccaggccat gtcgaacgcc gaggcccggc ttcacggcgt gggcatgctg
     6481 gcgaaggggg cgagccccgc ccaggtgatc gccgccgtgg cgaacaagga gttcgactcc
     6541 gactggcagg aacagcagta cggcgtcgcg gcgctcggct tcgaggacgc ctccgccgcc
     6601 ttcaccggcg ccggcaccta cgacgtgaag ggtgacctcc agggctacgg cgtctccgtc
     6661 cagggcaaca tcctgaccaa caaggaagtg atcgagcgcg cgctggccgc cttcctggcg
     6721 ccgccggcca agccggccac ctcccggtcg ctggcggagc ggttgctgtc ggccctcgaa
     6781 gcgggcgcgg aggccggggg cgacaagcgc tgcggcaagc agaccgccat gtcgtcctac
     6841 gtcgtggtgg cgaagccgga cgacgacccc gtgcacccct cgacgctcgt catcgtccgg
     6901 gaggacgagg gaggagccaa cccggtgcgc gtgctgaggg agcggtacga gcgcgcgagg
     6961 cagaagcggc aagggcactg agcagttccc ccactcggcc gttgcgtggt ggacgccgat
     7021 gatgcacctt cacctccggg tcgacgagaa cgcattcctg acggcggcgc gccggatcgc
     7081 ggaggagcag agcgtcttca cctggccgcg aaccacccct ggcgccacgc cttcggttcg
     7141 cgtcgtcgaa ttcacggccg gcgacgcgac tctcgggctc acggccgagg aggtggcggg
     7201 aatcgtggag caactcgtgg ccggatgatc ggcgagagga gggagaagct cccgctccga
     7261 gcttctcccc aggatcatca agcgagaatg cagtttccgc agcactgtcc tttgggggct
     7321 cgacagatgc tgccatccgg gcaggtagtg ccgacgaggc acatacccag actggaacaa
     7381 tcgatgcatg cgccgctgtc agcgggcatt cccaggaaga tggcgcagag tacgacggca
     7441 agggcgaaca gtctcttcat ggcttcctcc tttcagggcc actccatgcg gccctacaag
     7501 tgaaggtgca aaatatcgaa agatccgtgc cgctaacgtt agatcgtctt ccccaagctt
     7561 gactcgcctc agataacgct atctcgtctc agccaagctt agcctggccc agacacgctt
     7621 gagttgagcc cgccaagcgt atctggatct ggatagcctc atctcgcctc catcaagcct
     7681 ctctcgtcct ggataagctc agggagtccc gcagagcgct atctcgcccc ggataagctt
     7741 ggcgggaacc agctaagctc aactcggccc agataacgcc atctcatccc ggataagctt
     7801 agcgggctcc agataagctt atgtcgagcc gacctgagct cagttgagcc ggctcagctt
     7861 atctcgggcg ccagcgatag agctcgagcc gatagccctc cggatagcgg ccaggcggcg
     7921 agacgaggcg tacccgggtc tcgccaccgg tcagagcgaa cctccgtgtc atcagacgga
     7981 ccttgcgtgc ccgcgaaccc gcctcgaagg tacggacgag ctcccctccc ggatcgaggc
     8041 ggaggccttc gagccgctgg gcttcccggg gcgcccagag cacggccgac aggacgtctc
     8101 cgggggcgat gcccggcggc aggacggagg tctgcctgcg cctgacctgg gggggccgtc
     8161 cgagcggcag ctcgaaacgc ccgtcctggc gccacggatg aagaagaagg accagcgtct
     8221 cgccgcggct gtggaaaggc cggctcgaaa cgcgcacgac ctggtccggg gggaggcgct
     8281 cgaggcgccg gcgatagaag tcggcgtccg ggccgtggag ccggctgtcg gggaagacct
     8341 ccgcgtcggc ccgttcgagg aaggccgggc cgagctcgcc gagccggccg gccgccgtga
     8401 tcaacgcccg gttcttgccg ccgagacgga ggatgttcgt ccccttctcg tagaccacgt
     8461 gacgcagccc gatcggcgcg aggcgcgcgg tgaggacgtg gcgcgcctcg gcccaggtgt
     8521 tcgggatcac ccatttatag accaccgcgg cctggtccgc gaggaggacc aggccggcgg
     8581 ccaccacggc cgtggcggcg gcgggccgcc gggacagcgc gggtactcgg tggacgagtg
     8641 ccctccaggc ctcaaccatg gcccaggcgg cgatgagcga ggtgaacggc gccaccggca
     8701 ggtagttctg gctgcggaag agggtcatcc ccgccgtgtg gagggccgag tagccgagga
     8761 agagcgagag cacgaggagc caaccgaccc gtcgctcgcc gccgtctccc ggcacgggcc
     8821 gccggtagcg ccagaggagg ccgaccgtcc cggcgagcac gaagagggcc gtcagccagc
     8881 catggtgaac gatcaggaat tcgatctgcc ggatgaaaac cacccaatga ccgctcggct
     8941 cattctcgac gcgggccgcg tagctgctcg cctgcagcgg gatgtaatgt aagaccacgc
     9001 ccagaaaggg attcaagacg acgaaggtgg cgatcgagat gagtccggcg gccaccagcc
     9061 agccgagctg gcaccggtcg cgccgcggcc ccaggccgtt gcagagcgcc gccgcggtga
     9121 tcggcaggca ggaagcgatg ccggtgtact tggccgagac cgcgagcccg atgcctgccc
     9181 ctacccgcgc gtagcggccg agcgccggac ggaaggccgc ggcgagcgtc cagtagaacg
     9241 tgaccgtcgt gaggaagagc acgaggatgt ccggcttgaa ctgggtcgag gagaggacgt
     9301 ggcggtggag ggcggcgagc acggccgcgg ccagcagccc tgccgcgggc gaatggatcc
     9361 gccggccgat gaggaagacc atccacagcg cgaggacgcc gaagaggacg ttgcacagcc
     9421 ggcagaggag ataggccgta ggtgagtagc cgtccacggc cttctccgag aagatcgaca
     9481 gggcctcgat accggtcagc tcgtggagcc cccaactcgc ggcgagcacc gccgactgtg
     9541 gcaggtaaga gaggctcgga tagaaagcgt ggcgcggccg gatctccccc tgcaagagcc
     9601 cggtgacatt tcggaaggcg aagcgctcgt cgccgtagtg tccggtgtgg aggccctggc
     9661 aggccagcca gaggcggagg agaaagctca acagaaggag gacggcgagc agccaggaga
     9721 agagggggcc cgtgcgggcg ctctggctct ggtggtcggg atggagcgtt tccatcaatc
     9781 cacggggggt ccggttgtcg tgccgggact ggccgccctg ttgcaagtcg cagttgatga
     9841 tagcatgcgg cagaaagcaa gtcctctcag gatggaaggc gtggtttgca gagtgaaacg
     9901 cgacagccct ctcctccgta tacctctata gagagctccc atcccgagcc gtaggggaaa
     9961 ggacgggcca ctccatgagg agctgaccca tgctgcggct gacgcgcgtc atcgccttcg
    10021 cccttctctc gacccaggtc tactcctatg acgcgctgaa ccggctggcc tcggtgagcc
    10081 aaccgtggac cggagccggg agcaccgaag ccgtgaccac ctacggctat gacctccagg
    10141 cccatctcac ctcggtgacg gatgccgaag gcaatatcac tacgtacacc tacagcgacc
    10201 gtgacctgct gacccagcag acctcgcccg tctccggcgt gactactcat cagtacgatg
    10261 atcacggcga gctgatcgcg gagacggacg cgcggggtat caccgtcgaa cgagaggtcg
    10321 acatcctggg ccgagtggtc ctggtggact accccgacga ggcgctggac acgacctatt
    10381 cctatgacga ccccgcagtc cccttctcca aggccgactg acggcgatca cgcgcacggg
    10441 ctcgaccggg gaacgccctt cttcaaatgc gtcacccgtt gcctggaatc tttcgggtgc
    10501 ggaaattatt gaagtgaact atgaaccaca agacggccga acacatcatc tctgtatcga
    10561 tgagctgcat caaccggctc gataggtcgg tcgaggacgc catcgacgcc gcgccggagg
    10621 agcacgcctc ttcgtatcgg agcttggcag gggaggtgat gggatacctc ttcaccgagg
    10681 tgctaatgcc catctatgcg gctcaccccg atctcgagcc ggaggaactg agggaggcga
    10741 gagtgagctc acagcctaca tccatgccgc ccgagatcgc cgcaaagctg atgaatgttt
    10801 gtacctctgt gagccagtcg ctgagagacc tgtgcgaaga agtggctttg tcgcctcggg
    10861 agaccgactt tgaactcgaa tcagccctac aagagcccct taaggcactt cggcacactc
    10921 aagagttctt gcgtcgggcg tgcccagatc tcgagccagg atgaggtgtc cctgtcattg
    10981 aggtgtcaag ccgtcgaacc tcagacggac gcgccgtcgc ccccctcccc ccgcgccggc
    11041 gccacttgga ggttcttgaa gcgctccagc accacctttc ccgccggcag cccgcgcggt
    11101 ttgcggacgt cggcgcaggt gcagacgtgc acggcgaccc ggccgccgtt ccttgctttg
    11161 gagtagccgg cggcgaggga ggcggcgaag cgctcggtct cctttggcag gcgctcgagc
    11221 ccttcggggt tgcggactac aacgtgagag ccggagtccc cggcgatgtg catccagaaa
    11281 tcccgcggcg ctcccagctt gaaggtcagg acgtcgttgt cctcggcggt acggccgacg
    11341 aggaccgtca tgccgtccgg cgagacgaag cggcgcgcca cggagcgccc ctgccagagg
    11401 ccgttctcgg ggtccggagg gggcggcggg cccttcctcg ccatcaacgg tcctcccgcg
    11461 aggtgcgctc gaggaacgcc agccagccga tgagggcggc acgcagatcg tccagggaga
    11521 cccggcaagg cggcgcttcc ccgtcctcct cgtactcggc ctcgatggtg gcgcccgcgg
    11581 aatccaggat cagcgtgtgg gcgttgcccg tctcctgcca agacggctcc ttcccagcct
    11641 ctaccctttc aatggcgccg aggatgtccc gggcgtagtc ctcgctcccc tggatatcgc
    11701 tctccaggaa gcgcgccagc agctcttggg gctccgggct gttcgcgcgg gggtcgccgg
    11761 cctcgtcccg atagaagtgc atgacctgca tggtggtctc cctccctatc tatagatcgg
    11821 atacgcggtg ttgatgtcac cgcgcaggag atagccttcg atggtgaagc cgcgcccgga
    11881 tgggccgcgg aacctctcgg ccttcccggt cgtgcgctgg cgataggcgt tcaggatggc
    11941 gcggaccacc tcctcccggc tcaaggagtc ggggaacatg gtggaccgct tcccgagcca
    12001 gcggccgctc tctgggtcac ggatctcgac ccgcgcctcg tagaccccct gccggttggg
    12061 tcgcgagacg acctgagaca cccgggcgcc gcgcgggtcg cgaccgccgg gccgggagtg
    12121 gaagcccacc ggcctcccct tccggttgat ctccccctcg aacacgtggg ccgcgttgat
    12181 ccggggcttc gtcgcggtcc agcgcgcggc ggcctgcagg gcgccgagcc ccaccagggc
    12241 cgcgagcacc gcgcagacga ggatcaccaa ggcagaggat ctctgcggtc gtctcatcga
    12301 gatctcccgg gcgcgagccc gcttattctc cggtcgtccc cggacctccc ggggcacccg
    12361 gtcccccagg ccctcccggg ccgctgagct gggcgccagg ggcaccagga gctcccgggc
    12421 caccgggacc gatcagcggc gtccagggtg agcccggtcc ggggatcacc acccgcgggc
    12481 ccgggccatg gcctccgtca tgccctcccg ggccatggac atggaggtgg atgtggacgt
    12541 cctgctgcgc ggcggccggc ggcggcgcga gctgggagac gaaccagcgg aggtccacct
    12601 ccgagaggtc gcccgcctcc cccatctgct cgaccttcac gagctggccc ttggcgtccg
    12661 tggtcaacaa gtatctcgta gctctcgtca tgaccaaaac ctcctgtcgc ggtctaatgc
    12721 ctctggaggc cgccggcccg gaggtccggc ggcagcaagg ccagggtgac gatcttctct
    12781 ccactcgtga ggacggcgtc gagcgcctcg
//
LOCUS       DASPJI010000072         5464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_293539, whole genome shotgun sequence.
ACCESSION   DASPJI010000072 DASPJI010000000
VERSION     DASPJI010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293539"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1510)
                     /locus_tag="VEL74_01955"
     CDS             complement(44..1510)
                     /locus_tag="VEL74_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYG61319.1"
                     /translation="MKTRTVETSGPLERYPLEPGAEEMRRLVQEAMDRIVPHILSLPE
                     QPAADVEGAVDLARSLAEPLPLEGRPFPELLDLLFERAIPKSFNAASPGYLAYIPGGG
                     LFHTAVADLIADAVNRYTGVFAAAPALSQLEANVVAWFAEIFGYPRESRGILTSGGSL
                     ATLTALVTARRERLPENFLNGTLYVSDQTHHAVEKSALLAGFPPRNVREIPSDEAFRI
                     RLDALEQRIEEDRRQGFLPFLVVGNAGTTNTGAVDPLDELAAVALRQGLWLHVDAAYG
                     GFFLLTEEGRRLMRGIERSDSVVLDPHKGLFLPYGTGALLVRDGEALRRAHSVPAEYL
                     PPMQEDSDLVDFNEISPELSKPYRGLRVWLPLKLLGIGPFAEALEEKMELARWAAEEL
                     RALPGIEILAEPQVTVVAFRWNPGGIEAGRLDDLNRLNRRFLDRINARRRVMLTPTML
                     GGLFALRICVVSFRTHRDRVEAALEDIRAALVEMEGER"
     gene            1640..2239
                     /locus_tag="VEL74_01960"
     CDS             1640..2239
                     /locus_tag="VEL74_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HYG61320.1"
                     /translation="MSERLFEGDVLFLQRLLKSAGFYKSTLDGDWGPKTDAAVEAFDA
                     RFREIRDNIGEFDSRSEGNLHGLHPKAQEAARKFLRQVQDAGMTVRILSGTRTYAEQN
                     SLFRIGRFGDSRAKVTNARGGQSNHNFGIAWDIGVFIGGKYMTTSEPYRDAAEAGLFD
                     GLEWGGNWISFKDLPHYQLPTGLSISQLRGKFENGEPYA"
     gene            2244..2864
                     /locus_tag="VEL74_01965"
     CDS             2244..2864
                     /locus_tag="VEL74_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61321.1"
                     /translation="MKETALRLLLAAVLAVTAACSDRESGSTAARPEPPAAGRPSVPG
                     DAPSIEPNLEAITKAADVRCAEVRGRLDSYRKVERDLTGLSGLSAEGGELTAWLAGDR
                     PELIAAKAYGETGRAEYQLCYGEDGDLLVFRSEESRYDQPMGQVVETRVERFFFHDGK
                     LTRWTVGEQPPVAQTLTAAAGARASDLLELSRIFVEAARSPGAPPS"
     gene            2944..3702
                     /locus_tag="VEL74_01970"
     CDS             2944..3702
                     /locus_tag="VEL74_01970"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HYG61322.1"
                     /translation="MKGRVVAFLSAVLLSTSSAVAQPPSDRPQFALGIAAISSPEPYE
                     GADADILVVPAVSFSYKRFYFRGIVAGYRLWESGELSADLIARARARFGGYDEDDSPA
                     LEGMESRRKSADGGIELQWERERLLGVRLTPTTDLLDRSGGQQVTLDVYSPWRFGPVR
                     VEPGVGVEWQSEDLVNYYYGVRPEEARPGRPAFEPGSAVNLTAGVFVFTPVSRRLLLQ
                     TFLRYERLDSDIEESPIVDEGGALTAFAALSYAF"
     gene            3710..4645
                     /locus_tag="VEL74_01975"
     CDS             3710..4645
                     /locus_tag="VEL74_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYG61323.1"
                     /translation="MRRPGALPYLVLAAGVLITSTASILIRFAQAEGVPSLSIAAGRL
                     AIAALLLTPVAWSRAGADLRRVSRSDLLLAAGSGVFLAIHFATWISSLAYTSVASSVA
                     LVSTNPLWAGLASLVILRERLRGGTLAGILLTLGGTALILVSDSGAEAAARHPAPLLG
                     NLLALIGALAVTGYFLIGRALRQRLSLLAYIWLVYGTAALTLVMTAAATGRPLLGFSP
                     LAWLCIVGLALGPQLLGHSSFNWALRFLSATFVAVATLGEPVGSALLALAFFGESFAP
                     LQLAGFVLLLAGIYAAARSEQPDAGSPEEARPAGG"
     gene            complement(4669..>5464)
                     /locus_tag="VEL74_01980"
     CDS             complement(4669..>5464)
                     /locus_tag="VEL74_01980"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYG61324.1"
                     /translation="DEPCYQGPAPAPLVNPSGIKWIGDGTPIERLAFMSEPYQDRPET
                     RGRFNFSGDVFTSLVERGLFASPKKGQLLFHLVGDAAIDTLLDEMEELAPAAHWRHRR
                     TRIEHGDLMAPADVQRVKDLGVVVVQNPTHLVLPDIFQRYSPAVTAHAQPLRSLVETG
                     VPLAFGTDGIGGSISPFLDLLLATLHLDRPSEALTLEQALIVYTAGSAYAEHEEDKKG
                     KLVPGQLADLAVLSLDIFANPGALPATRSELTMVDGRIVWDTGVVH"
BASE COUNT          774 a         1980 c         1862 g          848 t
ORIGIN      
        1 ggctcccctg aacgggaggc gccagggcgg cgcctcccgt ccctcacctc tccccctcca
       61 tctccaccag cgccgcccgg atatcttcca gggccgcctc gacgcggtcc cggtgcgtcc
      121 ggaacgaaac cacgcagatc cgcagtgcga agaggccgcc cagcatcgtc ggagtgagca
      181 tcacccggcg ccgggcgttg atccggtcga ggaaccggcg gttgagccga ttgaggtcat
      241 cgagccgccc ggcctcgatc ccgcccgggt tccagcggaa cgccaccacc gtgacctgcg
      301 gctcggccag gatctcgatg cccgggaggg cccgcagctc ctccgccgcc cagcgggcga
      361 gctccatctt ctcctccagg gcctcggcga acgggccgat ccccaggagc ttgagcggca
      421 gccagacccg cagcccgcgg tagggcttgg acagctccgg cgagatctcg ttgaagtcga
      481 ccaggtccga gtcctcctgc atcggcggca ggtactcggc cgggacgctg tgggcgcgcc
      541 gcagcgcctc gccgtcccgc accagcaggg cccccgtgcc gtaggggagg aagagcccct
      601 tgtgcgggtc gagcaccacc gagtcggagc gctcgatccc ccgcatcaat cggcgcccct
      661 cctccgtcag caggaagaag ccgccatagg ccgcgtccac gtgcagccag aggccctgcc
      721 ggagggcgac ggcggcgagc tcgtccagcg ggtcgaccgc gccggtgttg gtggtgccgg
      781 cgttgccgac caccaggaag gggaggaatc cctggcgccg gtcctcctcg atccgctgct
      841 ccagggcgtc caggcggatc cggaacgcct cgtccgacgg gatctcgcgg acgttccgcg
      901 gcgggaaacc cgccaggagc gcgctcttct ccaccgcgtg gtgggtctgg tccgagacgt
      961 agagggtccc gttcagaaaa ttctcgggca gccgctcgcg ccgcgcggtc accagcgcgg
     1021 tcagggtggc gagcgacccg ccgctggtca ggatcccgcg ggactcgcgc ggatagccga
     1081 agatctcggc gaaccaggcg acgacattgg cctcgagctg ggagagggcg ggcgccgcgg
     1141 cgaacacgcc ggtgtagcgg ttgaccgcgt cggcgatgag gtcggccacg gccgtgtgga
     1201 agagcccgcc acccgggatg taggcgaggt acccggggct ggcggcgttg aacgacttgg
     1261 ggatggcgcg ctcgaacagc aggtcgagga gctcggggaa cggcctcccc tcgaggggga
     1321 ggggctcggc tagggaccgc gcgaggtcca ccgccccctc gacgtccgcc gcgggctgct
     1381 ccggcagcga tagaatgtgc gggacgatcc ggtccatcgc ctcctggacg aggcgccgca
     1441 tctcctcggc ccccggctcc aggggatatc gttccagtgg accgctcgtt tctacggttc
     1501 gggtcttcaa gctcgtctcc cttcgggggg agagggtatc cgaaagggtg gagcagccgt
     1561 aacgtcgccc cgagttccag cgcctcttgc tgagaagctc cagacccggg gcttcgcatc
     1621 tcatcgatcg gaggcccgta tgagtgaaag gcttttcgag ggcgatgtcc tctttctgca
     1681 acggctgttg aaatcggcag gtttttacaa gagcactctg gatggcgatt gggggcccaa
     1741 gacggacgcg gcggtggagg cgttcgacgc gcgcttccgt gagatccgcg ataatatagg
     1801 tgagttcgac tcgcgcagcg agggcaacct ccacggcctc caccccaagg cccaggaggc
     1861 ggcccggaag ttcctgcgcc aggtccagga cgccggcatg accgtccgca tcctctccgg
     1921 cacccgcacc tatgccgagc agaactccct cttccgcatc ggccgcttcg gcgactcgcg
     1981 ggccaaggtg accaacgcgc gcggcggcca gagcaaccac aacttcggca tcgcctggga
     2041 catcggcgtc ttcatcggcg gcaaatacat gaccacctct gagccctacc gcgacgccgc
     2101 cgaagccggc ctgttcgacg gcctcgaatg ggggggaaac tggatcagct tcaaggacct
     2161 cccccactac cagctcccca ccggcctttc catcagccag ttgcggggga agttcgagaa
     2221 cggggagccc tatgcctgag ggtttgaaag agaccgctct ccggctgctg ctggccgccg
     2281 tcctggccgt gacggctgcc tgctccgacc gggagagcgg ctccaccgcg gcccggcccg
     2341 agcctccagc ggcggggcgc cccagcgtgc cgggcgacgc gcccagcatc gagccgaacc
     2401 tcgaggcgat caccaaggcc gccgacgtgc gctgtgccga ggtgcgcggc cggctcgact
     2461 cctatcgcaa ggtggagcgc gacctgaccg ggctctccgg cctctccgcc gaggggggcg
     2521 agctcaccgc ctggctggcc ggcgaccgtc cggagctcat cgcggccaag gcctatggcg
     2581 agaccggaag ggccgagtac cagctctgct acggtgagga cggcgatctg ctggtgttcc
     2641 ggagcgagga gagccgctac gaccaaccca tgggccaggt ggtggagacg cgggtggagc
     2701 gcttcttctt ccacgacggc aagctgaccc gctggaccgt gggggagcag ccgccggtgg
     2761 cccagaccct caccgccgcg gcgggagcga gggcgagcga tctcctggag ctgtcccgga
     2821 tcttcgtcga ggccgcccgc tcccccggcg cgccgccctc ctgatccctg cccctgttcg
     2881 ccggtccttc cctggcagcc cgcatccatg ggaggatgac ggtcccatgg gagggagggt
     2941 ccgttgaaag ggagagtcgt cgcgtttctg tccgccgtcc tgctgtccac gtcctccgcc
     3001 gtcgcccagc caccttcgga caggccccag ttcgcgctgg gcatcgcggc catcagctcc
     3061 cccgaaccgt acgaaggcgc cgacgccgat atcctcgtgg tgccggcggt ctcattcagc
     3121 tacaagcgtt tctatttccg tggcatcgtc gcgggttacc ggctgtggga gtcgggagag
     3181 ctcagcgccg acctcatcgc ccgcgcccgc gcccgcttcg gcggctatga tgaggacgac
     3241 agccccgctc tcgaaggcat ggagagccgc cgcaagagcg ccgacggggg catcgagctg
     3301 cagtgggagc gggagcgcct cctcggcgtt cgcctgactc cgaccaccga tctcctcgac
     3361 cgcagcggcg gccagcaggt gacgctggac gtctactccc cctggcgctt cggcccggtc
     3421 cgcgtcgagc ccggcgtcgg cgtcgagtgg cagagcgagg acctggtgaa ttattactac
     3481 ggcgtccgcc cggaggaggc acggcccggc cgtccggcgt tcgagccggg ctcggcggtc
     3541 aacctcaccg ccggggtctt cgtcttcacc ccggtcagca ggcgcctgct cctccagacc
     3601 tttctccgct acgagcggct cgactcggac atcgaggaga gccccatcgt cgacgaggga
     3661 ggcgcgttga ccgctttcgc cgccctgagc tacgcgttct gaggaaccga tgcgacgccc
     3721 cggcgccctt ccctacctgg tgctcgcggc gggcgtcctc attacctcga cggcgtcgat
     3781 cctgatccgc ttcgcccagg cggagggggt accctcgctg tccatcgccg ccgggcgcct
     3841 ggccatcgcg gccctcctcc tcacccccgt tgcctggagc cgggccgggg cagacctgcg
     3901 gcgcgtcagc cggagcgatc tgctgctggc cgccggctcc ggcgtcttcc tggccatcca
     3961 cttcgccacc tggatcagct cgctggccta cacatcggtg gcgagcagcg tcgccctggt
     4021 ctcgaccaat ccactctggg ccggcctcgc ctccctggtg atcctccgcg agcgcctgcg
     4081 cggcggcaca ctggccggca tcctcctcac cctggggggg acggcgctga tcctcgtctc
     4141 cgacagcggc gccgaggccg cggcgcgcca ccccgcgccg ctcctgggca acctcctcgc
     4201 cctgatcggg gcgctcgcgg tgaccggcta cttcctgatc ggccgcgcgc tgcggcagcg
     4261 cctctccctg ctcgcctaca tctggctggt ctatggcacg gcggcgctga cgctggtaat
     4321 gactgccgcg gcgaccggcc ggccgctcct cggcttctcg cctctcgcct ggctctgcat
     4381 cgtggggctg gcgctggggc ctcagctcct cggccattcc tccttcaatt gggccctgcg
     4441 cttcctctcg gcgaccttcg tcgccgtggc gaccctcggc gaaccggtgg gctcggccct
     4501 tctggccctc gccttcttcg gcgagagctt cgccccgctc cagctcgccg gcttcgtgct
     4561 cctccttgcc gggatctacg cggcggcgcg gagcgagcag ccggacgcgg gatctcctga
     4621 agaagcgcgg cccgccggcg gatgaccggc gggccgcctc gcttcagctc agtgcaccac
     4681 gcccgtatcc cacacgatcc ggccgtccac catggtgagc tcgctacggg tggcggggag
     4741 cgcgccgggg ttggcgaaga tgtccaggga cagcaccgcg aggtccgcga gctggccggg
     4801 gacgagcttc ccctttttgt cctcctcgtg ctcggcgtag gccgagcccg ccgtgtagac
     4861 gatcagcgcc tgctccagcg tcagcgcctc cgacgggcgg tccagatgca acgtcgccag
     4921 caggaggtcg aggaaggggc tgatcgagcc gccgatcccg tcggtgccga aggcgagcgg
     4981 caccccggtc tcgaccagcg accgtagcgg ctgcgcgtgc gccgtcaccg ccggcgaata
     5041 gcgctggaag atgtcgggca gcaccagatg ggtggggttc tgcaccacca ccacgccgag
     5101 gtccttcacc cgctggacgt ccgccggagc catcagatcg ccgtgctcga tgcgggtgcg
     5161 gcggtgccgc cagtgcgcgg ccggcgccag ctcctccatc tcgtcgagca acgtatcgat
     5221 ggccgcgtcc cccaccagat ggaacaggag ctggcccttc ttgggcgatg cgaacaggcc
     5281 gcgctccacc aggctggtga acacgtcgcc ggagaagttg aagcgcccgc gggtttccgg
     5341 acggtcctga tagggctccg acatgaaggc gagccgctcg atgggcgtac cgtcgccgat
     5401 ccacttgata cccgatgggt tcaccagcgg cgccggcgcg ggtccctggt agcagggctc
     5461 gtcg
//
LOCUS       DASPJI010000073         3397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_464868, whole genome shotgun sequence.
ACCESSION   DASPJI010000073 DASPJI010000000
VERSION     DASPJI010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464868"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VEL74_01985"
     CDS             complement(<1..782)
                     /locus_tag="VEL74_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61325.1"
                     /translation="MRTTLDAFLICMALLLAAIASTTAATAQEVKFPLADYEALRARA
                     GMAPEPSPPPAAPFALESADFDIRVGEAVSARVVQRLSLTLFADGWQQVPLGEAGSFI
                     AADLGGLEGRVAATGESWSLHVRGKGRHEVRLESVVPLTRDETATRPVWRLRLRSPAA
                     AVVRGRIAAAGMTGTPEPIEPIEEVGLGGAGLARRGPDGAWSFVARPGTLVDIQLAGR
                     RTLPERARLPLRFETTSATAAAVSRTRLTVRSWIEARVAQGRL"
     gene            1087..2943
                     /locus_tag="VEL74_01990"
     CDS             1087..2943
                     /locus_tag="VEL74_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HYG61326.1"
                     /translation="MSSRPLLLSCESISKQFGIRPLFQNLSFGLFEGDRVGLVGPNGS
                     GKSTLLKILAGLEEPDTGTRSLRKGVRVGYVPQDPVFPAAMPAEEVLIQAMAGEHLEE
                     YEKSARAAMLLGRMGFRDPRQAAGSLSGGWQKRLAVARELVRTPDILLMDEPTNHLDL
                     EGILDLEEILASEASAYIVISHDRYFLENVARRMLELDRVYPEGLLQVDGTYSDLLER
                     RDEVRRNQAEYQESLANRARHEMEWLRRGPKARTTKAKARIQEAHRLIDELDEVKGRS
                     GESTASIDFAASGRKTKRLLLARGLAKSFADRQVLSGIDLLIRPGTRLGVLGPNGSGK
                     TTLLSILAGTLDPDAGEIERAQFLKTVLFEQSRDTLDRSVSLRRALAPEGDSVIYQDR
                     AVHVAGWAKRFLFRPEQLDTPVSRLSGGEQARILIARLMLQPADLLILDEPTNDLDIP
                     TLEVLEESLLEFPGGLVLVTHDRYLLDRVCTAILALDPDEDGRGGARLFADTGQWEEH
                     RRSLAAKPGAAPAAPKPVPKSANTEPAPAKTSSKPAAKRLTYREQKEWERMEEDIVAA
                     EEALARCQEAVEDPAVAADAAALNERCVALEAARAEVERLYARWAELEAKLA"
     gene            2949..>3397
                     /locus_tag="VEL74_01995"
     CDS             2949..>3397
                     /locus_tag="VEL74_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HYG61327.1"
                     /translation="MPHPPRPPRGRYAPSPTGMIHVGNARTALAAWLSARSRGGAFVW
                     RLEDLDGPRVVPGLAAAQQEDLAWLGLDWDEGPDRGGPHAPYAQSERSAVYEAALDRL
                     AAAGRLFPCRRSRKDLQSLASAPHGPEGLPPYPAAFRPQGLEAGWLAG"
BASE COUNT          484 a         1284 c         1202 g          427 t
ORIGIN      
        1 aggcgcccct gggccacccg cgcctcgatc cagctccgca ccgtgaggcg cgtccgcgac
       61 acggccgccg cggtggccga ggtggtctcg aagcggaggg ggaggcgcgc cctctcgggc
      121 agtgtccggc ggccggcgag ctggatgtcg acgagggtgc cgggacgggc gacgaaggac
      181 caggcgccgt ccgggccgcg ccgcgccagg ccggcgccgc cgagcccgac ctcttcgatc
      241 ggctcgatcg gctcgggtgt cccggtcatc ccggcggccg cgatccgccc ccgcaccacg
      301 gcggccgcgg ggctccgcag gcgcagccgc cagaccggcc gcgtcgccgt ctcgtcgcgc
      361 gtcaggggga ccaccgactc gagccgcacc tcgtggcggc ccttgccgcg cacgtgcagg
      421 gaccagctct cgccggtggc ggccacccgc ccctcgagcc cgcccaggtc ggcggcgatg
      481 aacgagcccg cctcgccgag cggcacctgc tgccagccgt cggcgaagag ggtgagggag
      541 agccgctgca ccacgcgggc gctgaccgcc tctcccacgc ggatgtcgaa gtccgcgctc
      601 tccagggcga agggggcggc cgggggcggc gagggctccg gcgccatgcc ggcgcgagcc
      661 cgcaacgcct catagtcggc gagtgggaac ttgacctcct gcgccgtggc cgccgtggtg
      721 gaggcgatag cggcgaggag gagcgccata cagatcagaa aagcgtcgag cgtcgttctc
      781 acggaacgga ctcctttcaa ggaacgctct ccgcttcaca agacaagact ccaacccacc
      841 gatcacgttc ccggatcaaa agtcatcggc gaaagccgga gcgtgcgatg gtcttcgatc
      901 gccagtgcaa aggccgtacc gattgtaaaa tcctgtaaaa acaggctttc ggcgccggga
      961 cggacggcgc gggtccaaga aaccggacgg ccggttcatt tccttggaca ccggatgcca
     1021 cgccaccgtc tataatcccc ctcctcccgg ccgggcctta cccgccggcc actgccttca
     1081 gacgccatga gctcccgtcc cctcctcctg agctgcgagt ccatctcgaa gcagttcggc
     1141 atccggccgc tgttccagaa cctctcgttc ggcctgttcg agggggaccg ggtggggctg
     1201 gtggggccca acggctcggg caagtcgacg ctgctcaaga tcctcgccgg cctcgaggag
     1261 ccggacaccg gcacccggtc gctgcgcaag ggggtgcgcg tcggctacgt gccgcaggac
     1321 ccggtcttcc cggcggcgat gccggccgag gaggtgctca tccaggcgat ggccggcgag
     1381 cacctggagg agtacgagaa gagcgcccgc gccgccatgc tgctggggcg gatggggttc
     1441 cgcgaccccc gccaggcggc gggcagcctc tcgggcggct ggcagaagcg cctcgccgtc
     1501 gcccgcgagc tggtgcggac gcccgacatc ctcctcatgg acgagccgac gaaccacctg
     1561 gacctcgagg gcatcctcga cctcgaggag atcctcgcct ccgaggcgtc ggcctacatc
     1621 gtcatcagcc acgaccgcta cttcctggag aacgtggcgc ggcggatgct cgagctcgac
     1681 cgcgtctacc ccgaaggtct cctgcaggtg gacggcacct actcggacct cctcgagcgg
     1741 cgggacgagg tgcgccgcaa ccaggcggag taccaggagt cgctcgccaa ccgcgcccgc
     1801 cacgagatgg agtggctgcg ccgcggcccc aaggcgcgga ccaccaaggc caaggcgcgc
     1861 atccaggagg cgcaccggct gatcgacgag ctcgacgagg tcaaggggcg gtccggggag
     1921 tcgaccgcca gcatcgactt cgccgcctcg ggccgcaaga cgaagcggct gctcctcgcc
     1981 cgcggcctcg ccaagtcgtt cgccgatcgc caggtgctct ccggcatcga cctgctgatc
     2041 cggccgggga cccggctcgg ggtgctgggc cccaacggca gcggcaagac gacgctgctg
     2101 tcgatcctcg ccggcacgct cgaccccgac gccggagaga tcgagcgggc gcagttcctc
     2161 aagaccgtcc tcttcgagca gagccgcgac acgctggacc ggtcggtcag cctgcgccgg
     2221 gccctggctc ccgaaggcga ctcggtgatc taccaggacc gcgcggtcca cgtcgccggc
     2281 tgggccaagc gcttcctgtt ccggccggag cagctcgaca cgccggtgtc gcgcctctcc
     2341 ggcggcgagc aggcgcgcat cctcatcgcc cggctgatgc tccagcccgc cgacctgctg
     2401 atcctcgacg agccgaccaa cgacctggac atcccgacgc tcgaggtgct ggaggagagc
     2461 ctgctggagt tccccggcgg cctcgtgctg gtgacccacg atcgctatct gctggaccgg
     2521 gtctgcaccg ccatcctggc gctcgatccc gacgaggacg gccgcggcgg cgcgcggctc
     2581 ttcgccgata ccggccagtg ggaggagcac cggcggtcgt tggctgccaa gccgggcgcc
     2641 gctccggccg cgccgaagcc ggtcccgaag tcggccaaca ccgagccggc cccggcgaag
     2701 acgtcctcca agccggccgc caagcgcctc acctaccgcg agcagaagga gtgggagcgg
     2761 atggaggagg acatcgtggc cgccgaggag gccctggccc gctgccagga ggcggtcgag
     2821 gaccccgccg tggccgccga cgccgccgcc ctcaacgagc gctgcgtcgc cctcgaggcc
     2881 gcccgcgccg aggtggagcg cctctacgcg cggtgggccg agctggaagc caagctggcg
     2941 taggccggat gccccaccct ccccgcccgc cgcgtggccg ctacgccccg tcgcccacgg
     3001 ggatgatcca tgtgggcaac gcccgcacgg cgctggcggc gtggctgtcg gcgcgcagcc
     3061 gggggggcgc gttcgtctgg cggctcgagg acctggacgg gccgcgggtg gtccccggcc
     3121 tggccgcggc gcagcaggag gacctcgcct ggctcggcct cgactgggac gaggggccgg
     3181 accgcggcgg cccgcacgcc ccctatgccc agtcggagcg ctcggcggtc tacgaggcgg
     3241 ccctcgaccg cctcgccgcg gccggccgcc tcttcccctg ccggcgctcg cgcaaggacc
     3301 tccagtccct ggcgtcggcg ccgcacggcc ccgaggggct ccccccctac cccgccgcct
     3361 tccgtcccca gggcctcgag gccggctggc tcgccgg
//
LOCUS       DASPJI010000074         3050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_519514, whole genome shotgun sequence.
ACCESSION   DASPJI010000074 DASPJI010000000
VERSION     DASPJI010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519514"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VEL74_02000"
     CDS             <1..456
                     /locus_tag="VEL74_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYG61328.1"
                     /translation="GTGGTISGTAKYLKEQNPAVQVIGVDTVGSIYKYYFEHRELPPE
                     DQIHQYLIDGIGEDFMPSCVWWDYIDGIVTVDDKSAYDATRELVRKEAIFVGSSAGAA
                     ASGARQVAESLPADNLVVTLFPDSGERYLSKINRHWMKEKGLIQSEEEF"
     gene            456..758
                     /locus_tag="VEL74_02005"
     CDS             456..758
                     /locus_tag="VEL74_02005"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018917055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HYG61329.1"
                     /translation="MYIAMNRFRVNAGFEEGFERMWQERDSYLAEVPGFRSFHLLRGT
                     TTEGITTYLSHSTWESREAFVAWTESDAFRKAHGDARSPQGTLAGHPLFEGYEVVL"
     gene            complement(755..1297)
                     /locus_tag="VEL74_02010"
     CDS             complement(755..1297)
                     /locus_tag="VEL74_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HYG61330.1"
                     /translation="MTQTSTAVAPLADGSNPRAEKLRSRMLSPFLMRGFFLAKLPLAL
                     VAGLRVRELTAERCIATVPYGWRTTNPFRSTYFAAQSMAAELSTGALAMLAVELSPQP
                     VAMLIVGLEATFEKKATALTAFTCEDGPRIFAAVEETLRTGEPATVRAETVGRMPDGT
                     VVARFAFTWSFKRRSAKGSA"
     gene            complement(1862..>3050)
                     /locus_tag="VEL74_02015"
     CDS             complement(1862..>3050)
                     /locus_tag="VEL74_02015"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HYG61331.1"
                     /translation="TVFLGGPPALVLSAIAPLPENVPELMLASLIAGERLPLVTGVGP
                     HPLIANAEIALIGKVPPRERRPEGPFGDHYGYYSLEHDYPVFHVERIARRRDAIYPAT
                     VVGKPRQEDYFIGDLLQELLSPLFPLVMPGVEALWSYGETGYHSLAGAVVKERYRREA
                     MASAFRILGEGQLSLTKFLLVADRPVDLKDFRATLVHLLERTNPETDLYVFSNLSMDT
                     LDYTGPKVNEGSKGIWLGLGDPVRDLPRGFTAPEIPQGLTDVQVFCPGCLVLGGPSYA
                     DDPGAAARFVAHPAFAGWPLLVLTDEPRRAAASPINFLWTTFTRFEPAADIHAAAQEV
                     VRHHLAYRAPILIDARMKPWFPKELFPDEETAATVTRRWKEYFPGGKVEMGDSATGHL
                     DRV"
BASE COUNT          514 a         1000 c         1079 g          457 t
ORIGIN      
        1 ggcaccggcg gcacgatcag cggcacggcc aagtacttga aggagcagaa ccccgcggtc
       61 caggtcatcg gcgtcgacac ggtcgggagc atctacaagt actacttcga gcaccgggag
      121 ctgccgccgg aggaccagat ccaccagtac ctgatcgacg gcatcggcga ggacttcatg
      181 ccgagctgcg tctggtggga ctacatcgac ggcatcgtca ccgtggatga caaaagcgcc
      241 tatgacgcga cccgggagct ggtgcgcaag gaggcgatct tcgtcggctc ttccgctggc
      301 gccgccgcct ccggcgcccg ccaggtcgcc gagtcgctgc ccgcggacaa cctggtggtg
      361 accctcttcc ccgactccgg cgagcgctat ctttcgaaga tcaaccgcca ctggatgaag
      421 gagaaggggc tcatccagag cgaagaggag ttctgatgta catcgccatg aaccgcttcc
      481 gggtgaacgc cggcttcgag gagggcttcg agcggatgtg gcaggagcgg gacagctacc
      541 tcgcggaggt gcccggcttc cgttccttcc atctcctccg gggcacgacc acggagggga
      601 tcaccaccta tctctcccac tccacctggg aatcccgcga agccttcgtc gcctggaccg
      661 agtcggacgc cttccgcaag gcccacggcg acgcccgctc cccccagggg acgctggccg
      721 ggcatccgct gttcgagggg tatgaggtgg tgctctaggc ggagcccttg gcgctccgcc
      781 gcttgaacga ccaggtgaag gcgaagcggg cgacgacggt gccgtccggc atgcggccca
      841 cggtctcggc gcggacggtg gcgggctcgc cggtgcgcag ggtctcctcc acggcggcga
      901 agatgcgggg gccgtcctcg caggtgaagg cggtcagggc cgtggccttc ttctcgaagg
      961 tggcctccag gccgacgatg agcatggcca cgggctgggg ggagagctcc acggccagca
     1021 tggccagcgc gccggtggac agctccgccg ccatgctctg cgcggcgaag taggtggagc
     1081 ggaacgggtt cgttgtccgc cagccgtagg ggacggtggc gatgcaccgc tcggctgtca
     1141 gctcgcggac ccgcagcccg gccaccaagg ccaaggggag cttcgccagg aagaaacccc
     1201 gcatgaggaa cgggctgagc atccgcgaac ggagcttctc ggccctgggg ttggagccgt
     1261 cggccagggg ggccacggcg gtcgaggtct gggtcatggg cggtgtcctc gtatagagac
     1321 tagcgcattt cggaggtccg gctcgggcgg agcgttctac ccgcgagtcc tcaccccccg
     1381 accccctctc ccatctcccg cccatccatg ccctgggaga gggggagaaa aacaagagcc
     1441 gaggctgttc tccccctctc ccggcggggg gcggttggga gatgggagag ggggtcgggg
     1501 ggtgaggact ccacgcgcag acctcacagc cctcgcacca cctccaagat cctatccaag
     1561 accgcctccg gtctctcgaa cacatcctcg ttccggaagc gcaggatggt gcagcctaga
     1621 gacctcaagt agaaatccct gttctcgtcg tgcgctctct ggcgaggatc ggtgtggact
     1681 ccaccgtcaa gctcgaccac cagcttcagc gcatgacagt agaagtcagc aacgtagatg
     1741 ctgaggggag actggcgccg gaacttgagg tcagcgagcc tgcgatcccg caataatttc
     1801 cacagggcct gctccgcgaa ggtgagtggg ctcctcagct cgcgagcccg tgcgctccct
     1861 ctcacactct gtccagatgc cccgtcgccg agtcccccat ttccaccttg cctccgggga
     1921 agtactcctt ccagcggcgg gtgacggtgg cggcggtctc ctcgtcgggg aagagctcct
     1981 tggggaacca gggcttcatg cgggcgtcga tcaggatggg cgcccggtag gcgaggtggt
     2041 ggcgcaccac ctcctgcgcg gcggcgtgga tgtccgccgc gggctcgaag cgggtgaagg
     2101 tggtccagag gaagttgatc gggctcgccg cggcgcggcg gggctcgtcc gtcagcacca
     2161 ggagcggcca gccggcgaag gcggggtggg ctacgaagcg ggcggcggcg ccgggatcgt
     2221 cggcgtagga ggggccgccc agcaccagac agcccggaca gaagacttgg acgtccgtca
     2281 gcccctgcgg gatctcgggc gcggtgaagc cccgcggcag gtcccgcacc gggtccccca
     2341 gacccagcca gatccccttc gagccctcgt tcaccttcgg cccggtgtag tccagggtgt
     2401 ccatcgagag gttcgagaag acgtagaggt ccgtctcggg attcgtgcgc tccaggagat
     2461 ggacgagggt cgcccggaag tccttgaggt ccacgggccg gtcggccacc agcaggaact
     2521 tggtcagcga gagctgcccc tcgcccagga tacggaacgc cgacgccatc gcctcccgcc
     2581 ggtagcgctc cttgaccact gctccggcca gcgagtggta accggtctcg ccgtaggacc
     2641 agagcgcctc gaccccgggc atcaccagcg ggaagagggg ggagagcagc tcctggagga
     2701 ggtcgccgat gaagtagtcc tcctgccgcg gcttgcctac cacggtcgcc ggatagatgg
     2761 cgtcgcggcg gcgggcgatc cgctccacgt gaaacaccgg gtagtcgtgc tccagcgagt
     2821 agtagccgta gtggtcaccg aacggaccct cgggacggcg ctcgcgggga ggaaccttgc
     2881 caatcagggc gatctcggca ttggcgatca gcgggtgggg acccacgccg gtgaccagcg
     2941 gcagccgctc gccggcgatg agggacgcca gcatcagctc ggggacgttc tcggggagcg
     3001 gcgcaatcgc cgacagcacc agggccggcg ggccgccgag gaagacggtg
//
LOCUS       DASPJI010000075         4228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_236743, whole genome shotgun sequence.
ACCESSION   DASPJI010000075 DASPJI010000000
VERSION     DASPJI010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236743"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VEL74_02020"
     CDS             <1..755
                     /locus_tag="VEL74_02020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYG61332.1"
                     /translation="LQGSRVFVTDLGGGAWTPGFQIDQSHWIAAHLPISGYAARHLHG
                     GNRRHHVIYGGVDLDRYPVRPRPEHDGSLVFLGRILPHKGIHFLIQGLPPGTTLHVIG
                     PIGDATYLERLRDLAQGKGVRFHHGLGDTEVVGHLQRAMALVHPTPVDGSGSAGVHEL
                     LGLAPLEAMACGCPVVASDAASLPEVVADEESGLIVPPNDPGAITAALARLAGDADLW
                     RRLSAGARRRVEERFTWDLTAARCLEAYACAS"
     gene            743..1933
                     /locus_tag="VEL74_02025"
     CDS             743..1933
                     /locus_tag="VEL74_02025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG61333.1"
                     /translation="MRVLMVIHTRWNRELGGPRAQIELGEELRRLGDTVEKLSYEDVF
                     PEERPSRLPAPLSTLAGYVRSNRSFAARARAWVRTHGGRFDVIDANQTDLPFPKRDLG
                     FSGLLVARSVGLIPAYEEFERMAARRWPEPFSARRAVHRALTWPGHHRRLRDVERSFR
                     HADLINVSNSDELENVAGAMGWGPKAVMFPFGLSDADHRAFREERLPAAERLAACTAA
                     FIGTWNARKGARDWPLIVERVRQRVPGARFLFLGTGIGREQVLRDFPAETRGALEVVP
                     SYDNARLPGLLARVTAGAFPGYLEGFGFTVLEKLAAGLPVVAYDAPGPREMLRHQAIR
                     TTVPVGDTEAFAARLAEVLTLTPERYGEISADSSRVAALFSWPEIARRTRETYLDRLE
                     RLRS"
     gene            1936..2604
                     /locus_tag="VEL74_02030"
     CDS             1936..2604
                     /locus_tag="VEL74_02030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG61334.1"
                     /translation="MDTADLYRHKEQGYFTVARHDLLEMLPRQAAGGLRLLELGAGSG
                     ATLRAAKAMGLASYTVGIDIVEPAAASDGGPAVDLFLCGNVETMELDLPRDFDAVICA
                     DVLEHLVDPWRVVARLAGHLRSGGVFLSSIPNTRNHRLLSKIVLHGDFGYEDAGLFDR
                     SHLRFFCRRNIREMFEGAGLVVETMETNMGAYGLRHKALDLLTLGRLHDFFVFQYRTR
                     ARKP"
     gene            2601..3542
                     /locus_tag="VEL74_02035"
     CDS             2601..3542
                     /locus_tag="VEL74_02035"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYG61335.1"
                     /translation="MTARLVSVALATWNGARYLRQQLDSVYAQTWPNLEVVVTDDAST
                     DGTAEILQEYAERRGLRFEVNPERHGLIRNFERAISLCRGDCIALCDQDDLWKPEKIE
                     RLMAGLGDATLVYCNTEEQMTPEGEVRPEPSFAPIQSFARAYGSGRPTRHLLAENWVV
                     SHGVLFRRELVAHALPIPPHQPYHDGWLALVASTLGGIRYLDERLQVYRQHPESLTWI
                     PPERRGRRSGRFRATWRERCRKETERLQDASTLPTLTASDRAFLAELLTYHRSGLSRG
                     LHWRSAVSGWRIAPYLATLHEKPGRWKLPVRALLGGL"
     gene            3532..>4228
                     /locus_tag="VEL74_02040"
     CDS             3532..>4228
                     /locus_tag="VEL74_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HYG61336.1"
                     /translation="MASSLRTMARPLARGGRRLTGGALEALVGALSALSAVSPRVESP
                     GEPRSILVLRNNDLGDLLIVTPLFEALRRRFPEARIAAAVGHWNVGALRGNPHLSEVL
                     VTDAPWFNKFTRQGTRAALRYLATSPQIRRLRGFDVGIDVLGSAWGSLLLLRAAIPYR
                     LGVRGYAGGHTGVQRWVEFDPWEHVGRRALRFAELLGATDLPPCRPQIFLDAAERAAG
                     EQAWGAGPRVAIAP"
BASE COUNT          560 a         1550 c         1488 g          630 t
ORIGIN      
        1 gcctccaggg gagccgggtc ttcgtcaccg acctgggggg cggcgcctgg acacccggct
       61 tccagatcga ccagtcgcac tggatcgcgg cccacctccc gatctccggc tacgcggcgc
      121 ggcacctcca cggtggcaac cggcgacatc acgtcatcta cggcggcgtg gacctggatc
      181 gatatcccgt gcggccccgg ccggagcacg acgggtccct ggtcttcctc gggcgcatcc
      241 tcccccacaa ggggatccac ttcctgatcc aggggctgcc gccgggaacc accctgcacg
      301 tcatcggccc tattggagac gccacctatc tggagcgcct gcgggatctg gcccagggga
      361 agggcgtccg gtttcatcac ggcctcggcg acaccgaggt ggtgggtcac ctccagcggg
      421 ccatggccct ggtccacccg acgccagtgg atggctcggg gtcggccggg gtccacgagt
      481 tactgggcct cgctccgctg gaggccatgg cctgcggctg cccggtggtg gcgagcgacg
      541 ccgcctccct acccgaggtg gtggccgacg aggagagcgg gctcatcgtc ccgcccaacg
      601 atcccggggc gatcaccgcg gccctcgcgc ggctggcggg cgacgcagac ctctggcggc
      661 ggctctcggc aggcgcccgg cgccgggtcg aggagcggtt cacctgggac ctgacggcgg
      721 cgcgctgcct ggaggcgtac gcatgcgcgt cctgatggtc atccacaccc ggtggaaccg
      781 ggagctgggc gggccgcggg cgcagatcga gctgggagag gagctgcggc gcctcggcga
      841 cacggtggag aagctcagct atgaggacgt ctttcccgag gagagaccct cccggctgcc
      901 ggcgccgctc tcgacgctcg ccggatacgt gcgcagcaac cggagcttcg ctgcccgggc
      961 ccgcgcctgg gtgcggaccc acggcgggcg cttcgacgtc atcgacgcca atcagaccga
     1021 cctgccgttc cccaagcggg acctgggctt ctccggcctg ctggtcgccc gctcggtggg
     1081 gctgatcccc gcctacgagg agttcgagag gatggcggcg cggcgctggc ccgagccgtt
     1141 ctcggcgcgc cgggcggtcc accgcgccct cacctggccc gggcaccacc ggcggctgcg
     1201 cgacgtggag cggagcttcc gtcacgccga cctcatcaac gtctcgaaca gcgacgagct
     1261 ggagaacgtg gccggcgcga tgggctgggg tcccaaggcc gtgatgttcc ccttcggcct
     1321 gtccgacgcc gaccatcgcg ccttccggga ggagcggctg ccggccgctg agcgcctggc
     1381 cgcctgcacc gcggcgttca tcggcacctg gaacgctcgc aagggagcga gggactggcc
     1441 gctcatcgtc gagcgggtgc ggcagcgggt ccccggcgcc cgcttcctct tcctgggcac
     1501 cggcatcggc cgcgaacagg tcctgcgcga tttcccggcg gagacccgtg gcgccctgga
     1561 ggtcgtcccc tcctatgaca acgcccgcct gccgggtctc ctggcgcggg tcaccgccgg
     1621 cgccttcccc ggctatctcg aggggttcgg cttcacggtc ctcgagaagc tggcggccgg
     1681 gctcccggtg gtggcctacg acgcgccggg tccgcgggag atgctccggc accaggccat
     1741 ccggaccaca gtaccggtgg gcgataccga agccttcgcc gcccgactcg ccgaggtcct
     1801 gaccctgaca cctgagcgct acggcgagat ctccgccgac agcagccggg tcgccgccct
     1861 cttctcctgg ccggagatcg cgcgccgcac tcgggagacg tatctcgaca gactggagcg
     1921 cctccggagc tgaccatgga taccgcggac ctctatcgcc acaaggagca gggctacttc
     1981 acggtcgccc ggcacgatct cctggagatg ctcccgaggc aggccgccgg cggcctgcgc
     2041 ctcctggagc tgggggcggg gtccggcgcg acgttgcggg cggccaaggc gatgggcctc
     2101 gcctcctata cagtagggat cgacatcgtg gagcccgcgg ccgcctccga cggcggaccg
     2161 gcggtcgacc tctttctgtg cggcaacgtc gagacgatgg agctggacct gccgcgggac
     2221 ttcgacgcgg tgatctgcgc cgacgtgctg gagcacctgg tggatccgtg gcgggtggtc
     2281 gcccgtctcg ccggccatct gcgctccggc ggcgtcttcc tgagcagcat ccccaacacc
     2341 cgcaaccacc ggctgctgag caagatcgtc ctccacgggg acttcggcta cgaggacgcg
     2401 gggctcttcg accggtcgca cctccgtttc ttctgtcgcc gcaacatccg ggagatgttc
     2461 gagggggcgg ggctggtggt cgagacgatg gagaccaaca tgggggccta tggcctgcgc
     2521 cacaaggctc tggacctcct gaccctcggc cggctgcacg acttcttcgt cttccagtac
     2581 cggacccggg cccgcaagcc gtgaccgctc gcctcgtctc cgtcgcgctg gccacctgga
     2641 acggcgcccg ctacctgcgg cagcagctcg acagcgtcta tgcccagact tggccgaatc
     2701 tcgaggtggt ggtgaccgat gacgcctcga ccgacggcac cgccgaaatc ctccaggagt
     2761 acgcggagcg ccgggggctg cgcttcgagg tcaacccgga gcggcatggg ctgatccgga
     2821 acttcgaacg cgccatctcc ctctgccggg gggactgcat cgccctctgc gaccaggacg
     2881 acctctggaa gccggagaag atcgagcggc tcatggccgg gttgggcgac gcgacgctgg
     2941 tctactgcaa caccgaggag cagatgactc ccgaaggaga ggtacggccg gagccctcct
     3001 tcgccccgat ccagagcttc gcccgggcct atggcagcgg ccggcccacc cgccacctgc
     3061 tggccgagaa ctgggtggtg agccacggcg tcctgttccg ccgggagctg gtcgcgcacg
     3121 ccctgccgat cccgccgcac cagccctacc acgacggctg gctcgccctg gtggcctcga
     3181 cgctcggcgg catccgttat ctggacgagc gccttcaggt ctatcgccag caccccgaga
     3241 gcctgacctg gatcccgccg gaacggcggg ggcggcggag cggccggttc cgcgccacct
     3301 ggagggagcg ctgccggaag gagacggagc ggctccaaga tgcctccact ctcccgacgc
     3361 tcaccgcctc cgaccgcgcc ttcctggccg agctgctgac ctaccatcgc tcgggcctga
     3421 gccgcggcct ccactggcgc tcggccgtga gcggctggcg gatcgcgccc tacctcgcca
     3481 ccctgcacga gaagccgggg cgatggaagc ttcccgtgag ggccttgctc ggtggcctct
     3541 agcctccgga ccatggcgcg ccctctggcg cggggcggcc ggcggctgac cggcggagcg
     3601 ctggaggcgc tcgtcggcgc cctgtctgcg ttgtccgcgg tctctccccg agtggagtcc
     3661 cccggcgagc cgcgctccat cctggtgctg cgcaacaacg acctggggga cctgctgatc
     3721 gtcacccctc tgttcgaggc gctccgccgc cgcttcccgg aggcccgcat cgccgccgcc
     3781 gtagggcact ggaacgtcgg ggcgctccgg ggcaatcccc acctctccga ggtcctggtg
     3841 acggacgccc cctggttcaa caagttcacc cgtcagggga cccgcgccgc cctgcgctac
     3901 ctcgctacct cgccccagat ccggcggctg cgtggtttcg acgtcgggat cgacgtgctg
     3961 ggaagcgcct gggggtcgct gctcctgctg cgcgcggcca tcccataccg gctcggggtt
     4021 cggggctacg cgggcggcca cacgggcgtg cagcgctggg tggagttcga cccctgggag
     4081 cacgtgggga ggcgggccct ccgcttcgcc gagctgctcg gggccacgga cctccccccc
     4141 tgccggccgc agatctttct ggacgcggcg gagagagcgg cgggcgagca ggcgtgggga
     4201 gcgggcccgc gggtggcgat cgccccgg
//
LOCUS       DASPJI010000076         6385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_237234, whole genome shotgun sequence.
ACCESSION   DASPJI010000076 DASPJI010000000
VERSION     DASPJI010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237234"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VEL74_02045"
     CDS             complement(<1..351)
                     /locus_tag="VEL74_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61337.1"
                     /translation="MNKRLWLGLLLSLAAFQILGAAIAFVRDDAAGSTAFADLGFPPS
                     FYWIPLLACAAAGCWLLLAGKDDQRAVELGVFFVLLATPFANRWLLVLGQAVPAIWSW
                     PVLVLRNLQADAFFP"
     gene            complement(395..739)
                     /locus_tag="VEL74_02050"
     CDS             complement(395..739)
                     /locus_tag="VEL74_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61338.1"
                     /translation="MATDSTHQRMEQVMSAISEGTAGAAMSQRARDWLLVRYTRWMGK
                     RRIRDDERTPEEAWSEYESAFLTKFREIGSKAAELSGGSEIDQSAVRTAALTVEASTP
                     SPCPFCQPPDVP"
     gene            complement(953..1558)
                     /locus_tag="VEL74_02055"
     CDS             complement(953..1558)
                     /locus_tag="VEL74_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HYG61339.1"
                     /translation="MRNRRTLFAAALLLAGSVVASAPALAVATVGEAAPGFQLKDLSG
                     KTHSLADYKGKVVVLEWLNPNCPVSDRHAREKTMTELAKKHGEVVWLGINSTNKEHRD
                     FLQAAEHQAWAKKNGIQYTILYDETGKAGKAYDAKTTPHMFIVDKQGRLAYNGAIDDD
                     PSGRKAKAERANYVGGGITAQKAGKSIDPATTKPYGCSVKY"
     gene            complement(1551..2921)
                     /locus_tag="VEL74_02060"
     CDS             complement(1551..2921)
                     /locus_tag="VEL74_02060"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HYG61340.1"
                     /translation="MTPFRPAFLALAAATLLGLTGGASVAWGAASDWAVNEQSRVRLI
                     TPDKVASPSGELRFGLHFTLAPGWHVYWKNSGDAGFPPVVEIQEPKGLGEPEILWPAP
                     HRFELPGDLVAFGYEDEVVYPVRAALPSGGATGDRLDLSVEVDYLVCEVDCIPYRYTL
                     KLAQPLGAQPEPDPETGALFQRWVDSVPAPVERLPGVSTDGALVAGTAPALEVRVRGA
                     SGDPAGVGLFLESQDTFNTGKPEARTDGDGLVIRVPLQLLDAGKPLPQTATFAWTVTG
                     LKSADGRPVSFEARREVRQQSAPQTPEAQPAGGWSWLREPRVAALLVVVLALLALNLW
                     GLLAVPLPPSLLGARGLVHGVLGFVATGLAVWLLSTLSRQVSSAGLAAVELALLGMAL
                     FAWLWHRSAGKSGLKFVLALGLALCAATVPWLADRNRLSPTPGAADAVETASSSRTTY
                     GGTRDA"
     gene            2987..4393
                     /locus_tag="VEL74_02065"
     CDS             2987..4393
                     /locus_tag="VEL74_02065"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HYG61341.1"
                     /translation="MYVYFTNLGCKLNQAEIESLAREFLAAGHSVAGSLEEADLHVVN
                     SCTVTHVAARGSRKAAGRGRRAGLPLRTVLTGCWVTAEPEEAARLAGVDLVVANPEKD
                     RLLEHVHRAFPELALPEVPVPYVPLPFGNTRALLKVEDGCNMRCAFCIIPSTRGRQRS
                     RPPAELVEELKGLVRAGYREIVVTGVQISAYRSGADCLHELTRALLAALPSEEDGGVR
                     LRLTSIAPWDLPRDRERLLGLWTDRRLCRHLHLSLQSGSATVLRRMRRPYTAEGYLRL
                     LERVRAAIPGVAITTDVIAGFPGETAAEHAESLATVEAAGFAKVHVFPFSARPGTEAA
                     DMPGQIGPEEKKARVGQLLEAAARAEAAFRRAHLGQRATVLWEKPRDGMGHGLTDNYL
                     RVLCRDGAALWNRWSEVELVGLAEEGMVGEIPGGPSAPDSIRTLRTPSEVSGKLPEPS
                     GLHPIESDIVRSARTPAE"
     gene            complement(4548..5864)
                     /locus_tag="VEL74_02070"
     CDS             complement(4548..5864)
                     /locus_tag="VEL74_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61342.1"
                     /translation="MSEPQVPLVAPLVASVEMGYGHLRAAHALASALGTEVVHADQDP
                     MAPPDEQRLWRASRRFYEITSRTSQLPVVGVPLRSLLETLTDIPHLYPHRDLSAPTFQ
                     VRSLDRLVRKGLGRGLAEHLKRTGQPLLTTFFAPAIAADHHAGGERGKAFCVVTDSDI
                     NRAWVPLRPERTGIVYLTPSRRALKRLRAYGVARDQIEFTGFPLPPGLLGGPDLPVLR
                     RNLAARLVRLDRKRVFRGQLRNEIHHFLGDLPADQEGRPPLLTFSVGGAGAQAELGRR
                     LLVSLAAMIRERRVRLCLVAGVRSEVAWRFHRWIDEAGLGAHLRDGVDVFLAGALEEY
                     FPRFNALLAETDILWTKPSEMTFFAALGLPLILSWPVGVHERYNRRWAIENGSGLKQR
                     HPDYAGYWIREWLAEGTLAAAAWSGFMRMPKFGVYQIVDRVMGRAG"
     gene            complement(5857..>6385)
                     /locus_tag="VEL74_02075"
     CDS             complement(5857..>6385)
                     /locus_tag="VEL74_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HYG61343.1"
                     /translation="AILLSVRRWESALMTRLMRTLTHLGDTASWMVTGLVLAAAGHPR
                     WCALLATGAGLAVVVSQVLKRLCCRARPSCGLGGFAALVENPDAFSFPSGHTAAAFGI
                     AMALAGEGTALGALVLVLATGIALSRIYLGAHYPLDVAAGVIVGSLSGLAARLLIGSL
                     IDEPLAGYAILALGV"
BASE COUNT          955 a         2194 c         2344 g          892 t
ORIGIN      
        1 agggaagaag gcgtccgcct gcaggttgcg cagcaccagc acgggccagc tccagatcgc
       61 cggcaccgct tggccgagga cgagcagcca gcggttggcg aaaggggtgg cgaggagcac
      121 gaagaacact ccgagctcga cggcgcgctg atcgtccttg cctgcgagga gcagccagca
      181 ccccgccgcg gcgcaggcca aaaggggtat ccaatagaag ctggggggga aaccgagatc
      241 agcaaacgcc gtcgaaccgg ccgcgtcgtc ccgcacaaat gcgatcgccg cccccaggat
      301 ctggaaggcg gcgagggaca ggagaaggcc gagccagagc cgcttgttca aatcaccccc
      361 acctgctcgg gtcgacgttg aagcgaagtc ttctctacgg cacgtcgggc ggctggcaga
      421 acgggcaagg ggagggggtc gacgcctcta ccgtgagagc cgccgtcctg accgcgctct
      481 ggtcgatctc gctgccgccg ctcaactccg ccgccttgct gccgatctcc ctgaacttgg
      541 tgaggaaggc cgactcgtac tcactccagg cctcctccgg cgtgcgctcg tcgtcgcgga
      601 tcctgcgctt gcccatccac cgggtgtagc gcacgagcag ccagtcgcgg gcccgctgcg
      661 acatcgcggc acccgccgtg ccctccgaga tcgccgacat cacctgctcc atccgctggt
      721 gcgtgctatc ggtcgccatg tgtgctctct cccttcgtgt agagatgcct tcttggctgg
      781 attcatttca cccgacggcc cgggcgcggg ccatgagtga acgcatcact tttcagcttt
      841 cgttcactat agtcctcggc gtctctttct gtcaaggaac acagggcatg caaaaaggcc
      901 cgggcggagc gcccgggcct ctttttccaa cgaatgatcg attttcgatt tattaatatt
      961 tgacgctgca gccgtacggc ttggtggtcg ccggatcgat gctcttgccg gccttctggg
     1021 cggtgatgcc gccgccgacg tagttggccc gctcggcctt ggccttgcgg ccgctcggat
     1081 cgtcgtcgat ggcgccgttg taggcgaggc ggccctgctt gtcgacgatg aacatgtgcg
     1141 gcgtggtctt ggcgtcgtac gccttgccgg ccttgccggt ctcgtcgtag aggatggtgt
     1201 actggatgcc gttcttcttc gcccaggcct ggtgctcggc ggcctgcagg aagtcgcggt
     1261 gctccttgtt cgtgctgttg atgccgagcc agaccacctc gccgtgcttc ttggccagct
     1321 cggtcatcgt cttctcgcgc gcgtggcggt cggagacggg gcagttgggg ttgagccact
     1381 cgagcaccac gaccttgccc ttgtagtcgg cgagggagtg ggtcttgccg gagaggtcct
     1441 tgagctggaa gccgggggcg gcctcgccga cggtggcgac ggccagggcc ggggcgctag
     1501 ccacgacgga gcccgccagg agcagggcgg cggcgaacag tgtccttcga ttacgcatca
     1561 cgtgtacctc cataagttgt cctcgatgag gaagcagttt cgacggcgtc cgcggccccg
     1621 ggggtgggcg acagccggtt ccggtccgcc agccacggta cggtggcggc gcacagggcg
     1681 aggcccaggg cgaggacgaa ttttagacca gacttgccgg cgctccgatg ccagagccag
     1741 gcgaacagag ccatgcccag cagcgccagc tcgaccgcgg ccaggccggc cgagctcacc
     1801 tgccggctca gggtcgacag cagccagacc gcgaggccgg tcgcgacgaa ccccaggacg
     1861 ccgtggacga gcccccgcgc gcccagcagg gaggggggca gcggcaccgc cagcagcccc
     1921 cagagattga gggcgaggag ggccaggacg acgaccagca gcgccgcgac ccggggctcc
     1981 cgcagccagg accatccgcc ggcgggctgc gcctccggcg tctgcggggc gctctgctgc
     2041 cggacctcgc ggcgcgcctc gaagctcacc ggccgtccgt ccgcggactt gagaccggtc
     2101 acggtccagg cgaacgtggc agtctgcggc agcggcttgc cggcgtcgag gagctggagc
     2161 ggcacccgga tcaccaggcc gtcgccgtcc gtccgcgcct cgggcttgcc ggtgttgaac
     2221 gtgtcctggc tctccaggaa gaggccgacg ccggccgggt cgccgctcgc gccgcgcacc
     2281 cgcacctcca gggcgggcgc cgttccggca acgagggcgc cgtcggtgga gacgccgggc
     2341 agccgctcga ccggcgcggg cacgctgtcc acccagcgct ggaagagcgc cccggtctcc
     2401 gggtcgggct ccggctgggc tcccagcggc tgggcgagct tcaaggtgta gcggtacggg
     2461 atgcagtcga cctcgcagac caagtaatcg acctcgacgc tcagatcgag gcggtcgccg
     2521 gtggcacctc cggagggaag cgcggcacgg acgggataga ccacctcgtc ctcgtagccg
     2581 aaggcgacga ggtcgccggg cagctcgaag cggtggggcg cgggccagag gatctcgggt
     2641 tcgccgagcc ccttcggttc ctggatctcc accaccgggg ggaagccggc gtcgccgctg
     2701 ttcttccagt agacgtgcca gcccggcgcg agggtgaagt gcaggccgaa ccgcagctcg
     2761 ccgctcgggg aggccacctt gtcgggggtg atcaggcgca cccgggactg ctcgttgacc
     2821 gcccagtcgc tcgcggcacc ccaggctacg gacgctcccc ccgtcagccc caggagggtg
     2881 gcggcggcaa gggcaaggaa tgcgggtctg aatggggtca taggccggtc tgctcgggta
     2941 ttctgcggaa cgacgacgga gaggccggaa ttcccggccc gaaaccatgt acgtttattt
     3001 tacaaatctg ggctgcaagc tgaaccaggc cgagatcgag agcctcgccc gggagttcct
     3061 ggcggcgggc cactcggtgg cggggtcgct cgaggaggcg gacctccacg tggtcaactc
     3121 ctgcacggtg acccacgtgg cggcccgcgg ctcccgcaag gcggcggggc ggggacggcg
     3181 cgccggactc cccctgcgca cggtcctcac cggctgctgg gtgaccgccg agccggagga
     3241 ggcggcgcgc ctcgccggcg tcgacctggt ggtggccaac ccggagaagg accggctcct
     3301 cgaacacgtc caccgggcgt tccccgagct ggccctgccg gaggtgccgg tcccgtacgt
     3361 ccccctgccg ttcggcaaca cccgggcgct gctcaaggtg gaggacggct gcaacatgcg
     3421 ctgcgccttc tgcatcatcc cctcgacccg ggggcgccag cgcagccggc cgcccgccga
     3481 gctggtggag gagctgaagg ggctggtccg cgcggggtac cgggagatcg tggtgaccgg
     3541 ggtccagatc tcggcctacc ggtcgggagc ggactgtctc cacgagctga cgcgggcgct
     3601 gctcgccgct ctgccttctg aagaggatgg cggcgtgcgc ctgcgcctca cctccatcgc
     3661 cccctgggat ctcccgcgag atcgtgagag gctgctcggc ctgtggaccg accgccgtct
     3721 ctgccgtcac ctccatctct ccctccagag cggctcggcc accgtgctcc gccgcatgcg
     3781 ccgcccctac acggcggagg gctacctgcg gctgctggag cgggtgcgag ccgccatccc
     3841 gggcgtggcc atcaccaccg acgtcatcgc cggcttcccc ggggagaccg cggccgagca
     3901 cgcggagagc ctcgccaccg tcgaggccgc cggcttcgcc aaggtccacg tcttcccctt
     3961 ctcggcccgt ccgggcaccg aggccgcgga catgccgggc cagatcggcc ccgaggagaa
     4021 gaaggcccgc gtcgggcagc tcctggaagc ggccgcccgt gccgaggccg ccttccggcg
     4081 cgcccacctc ggccagaggg ccaccgtcct ctgggagaag ccgcgcgacg gcatgggcca
     4141 cggcctgacc gacaactacc tgcgggtgct ctgccgcgac ggggccgccc tctggaaccg
     4201 gtggagcgag gtggagctgg tcgggctggc ggaggagggg atggtggggg agatcccagg
     4261 cggtccgagc gccccggatt ccatccggac gctccggacg ccatccgaag tgtccgggaa
     4321 gcttcccgag ccgtccggac tccatccgat tgaatcggac atcgtccgga gcgcccggac
     4381 tccagccgag tgatgtggac gcgctccggc gcgctcggca ggcttccgga ccgtctggaa
     4441 ggcttccgag aggcgcggag gccatccgga gcgtccggga agctcccggg agcttccgga
     4501 cggcatccag gcgccgtgga tgcgctccgg agcgtccgag caggtcccta cccagcccgc
     4561 cccatcaccc ggtcgacgat ctggtagacc ccgaacttgg gcatgcgcat gaagccggac
     4621 caggcggcgg cggcgagggt gccttcggcg agccactcgc ggatccagta gccggcgtag
     4681 tcggggtggc gctgcttgag gccgctgccg ttctcgatcg cccagcgccg gttgtagcgc
     4741 tcgtggaccc ccaccggcca ggagaggatc agggggagcc ccagcgcggc gaagaaggtc
     4801 atctcgctcg gcttggtcca gaggatgtcg gtctccgcca gcagcgcgtt gaagcgcggg
     4861 aagtactcct cgagcgctcc ggccaggaag acgtcgacgc cgtcgcgcag gtgagcgccg
     4921 agccccgcct cgtcgatcca gcggtggaag cgccaggcca cttcggaccg caccccggcc
     4981 accaggcaga ggcggacgcg gcgctcgcgg atcatcgcgg ccaggctcac gaggaggcgc
     5041 cgccccagct cggcctgcgc gccggcgcca cccaccgaga aggtgaggag cggcggcctc
     5101 ccctcctggt ccgccggcag atcgcccagg aaatgatgga tctcgttgcg gagctggccc
     5161 cggaagaccc gtttgcggtc gagccggacc aggcgggcgg ccaggttgcg gcgcagcacc
     5221 ggcaggtccg gtccccccag gaggccgggg ggcaggggga agccggtgaa ctcgatctgg
     5281 tcgcgggcga cgccgtaggc gcgcagccgc ttgagcgccc ggcggctcgg cgtcaggtag
     5341 acgatgcccg tccgctccgg gcgcaggggg acccaggcgc ggttgatgtc cgagtcggtc
     5401 accacgcaga aggccttgcc gcgctctccg ccggcgtgat ggtcggcggc gatggcgggg
     5461 gcgaagaagg tggtcagcag cggctgcccc gtcctcttca gatgctcggc gagcccccgg
     5521 cccagcccct tgcggaccag ccggtccagg gagcgcacct ggaaggtggg ggcggagagg
     5581 tcccggtgcg ggtagaggtg ggggatgtcg gtcagtgttt cgagcagcga gcgcaacggc
     5641 acgccgacca ccggtagctg cgaggtgcgg gaggtgatct cgtagaagcg ccgggaggcg
     5701 cgccagagcc gctgctcgtc gggcggcgcc atggggtcct ggtcggcgtg gaccacctcc
     5761 gtgcccagcg ccgaggccag ggcgtgggcg gcccgcagat ggccgtagcc catctccacc
     5821 gacgccacga gaggcgcgac gaggggaacc tgcggctcag acaccgaggg cgaggatagc
     5881 gtagccggcg agcggctcat cgatcaggct gccgatcagg aggcgcgccg cgaggcccga
     5941 caggctgccg acgatcacgc ccgcggcgac gtcgagcggg tagtgggccc cgaggtagat
     6001 gcgggagagg gcgatgccag tggcgaggac gagcaccagc gcaccgaggg cggtcccctc
     6061 gccggccagg gccatggcga tgccgaaggc ggcggcggtg tgccccgagg ggaaggagaa
     6121 ggcgtccggg ttttcgacca gcgccgcgaa gccgccgagg ccgcagctcg gccgggcgcg
     6181 gcagcagagg cgcttcaaca cctgcgagac gacgaccgcc aggcccgccc cggtggccag
     6241 cagggcgcac cagcgcgggt ggccggcggc ggccagcacc aggccggtca ccatccagct
     6301 cgcggtgtct cccaggtggg tgagggtgcg catcaggcgg gtcatcagcg ccgactccca
     6361 gcgccgcacc gagaggagga tcgcc
//
LOCUS       DASPJI010000077         6901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_294036, whole genome shotgun sequence.
ACCESSION   DASPJI010000077 DASPJI010000000
VERSION     DASPJI010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294036"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..2125
                     /locus_tag="VEL74_02080"
     CDS             98..2125
                     /locus_tag="VEL74_02080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009020063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYG61344.1"
                     /translation="MKSLVLALCLAMASPMAGQPRLLTPDDVYHLKTVREPQVSPDGR
                     WVAYTVERLDRDEDTLDRDVWMVPYAGGEPVRLTASPKSERSPRFSPDGRYLAFLSSR
                     EGEKSQVWLLDRRGGDAVRLTDYKADVAELAWSPDGKRLALVVSDVDPDEEQDEGEDG
                     EEKEDRPKPIVIRRLQFKRDGEGYLREIRSHLYVFDVTSKTSTQVTSGPYDQSEPVWS
                     PDGQWIAFTSNRSTPDPDANENTDVFVVAAKAGATPRPVTTSPGGDSQPAFSPDGRLL
                     AYVAGGDPGDLWYGASHVAVVPVAGGPSKPLTQSLDRNVQSPLFSPDGRHVLFLVEDG
                     GNQHVARVPVAGGAVERVVAGEREVEAFDVGPKGELAVLESSPHQPAEISAAETAAVG
                     GAPRRLTRVNDAFLAGIRLGSVRRYKAKSADGTPVDAFLTLPPDAPQGRRLPTVLRIH
                     GGPTAQYSTGFKLEWQILAARGYAVIGSNPRGSTGYGRDFSRAIWADWGNKDFQDVMA
                     AVDEAVAMGVADPDRLGVGGWSYGGILTNYVIVQTDRFKAAISGASEVNYLSNYGTDH
                     YQYAWETELGLPWRNTELWIRLSPWFRVEKAVTPTLFVCSGDDVNVPCLNSEQFYQAM
                     RRLGRDTELVIYPGQTHAVRRPSFQKDRFERYIAWYDKYLKPEGAKAAAGR"
     gene            2232..2384
                     /locus_tag="VEL74_02085"
     CDS             2232..2384
                     /locus_tag="VEL74_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61345.1"
                     /translation="MDDLIAILIAAAFFVVFYLFVKAKERRERDPGISLFGGEPTDPP
                     PPEERQ"
     gene            complement(2378..3910)
                     /gene="proS"
                     /locus_tag="VEL74_02090"
     CDS             complement(2378..3910)
                     /gene="proS"
                     /locus_tag="VEL74_02090"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HYG61346.1"
                     /translation="MAKEETTAITPTRAEDYAEWYQQIIRAADLAESSPVRGCMVIKP
                     WGYTLWENIQREMDAMFKATGHRNAYFPLFIPKSFLEKEAEHVQGFAKECAVVTHYRL
                     IQDEKGELVVDPEARLEEPLIVRPTSETIIGEMFAKWVQSYRDLPLLINQWCNVVRWE
                     LRTRMFLRTAEFLWQEGHTAHETAEEAMEETRKMLDVYRDFSEDYLAVPVIRGRKTES
                     ERFPGADETFTIEAMMQDRKALQAGTSHFLGQNFAKASGIKFQSQAGTEEYAWTTSWG
                     TSTRMIGGVIMTHSDDNGFVVPPRVAPAHVVVLPILRDDSRQKVLDYVAEIAAELRRT
                     FYGNRPVEVEVDSRDIRGGQKKWDWVKKGIPIRLEIGPRDVDSGVVSFARRDQAVNQT
                     QAVPRAELAATVARVLEEIQAGLLAKAKEFRAQHMRPIGDWNEFTGFFTAKNADKPEI
                     HGGFAVSPWCGSADCETKVKEQLKVTIRCIPLDEVPDGGFDRCVVCGGEKKETAIFAK
                     NY"
     gene            4061..>6901
                     /locus_tag="VEL74_02095"
     CDS             4061..>6901
                     /locus_tag="VEL74_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HYG61347.1"
                     /translation="MVFYADLHVHSRYSRATSRDADLEHLALWARRKGITVVGTGDFT
                     HPAWLEEIRTKLVPAEPGLFRLRPEIEREVERELPPSCRGPVRFLLEVEISTIYKKGD
                     RTRKVHHLLYASNLETVARMRERLGAIGNLNADGRPILGLDSRHLLEITLESGPDAYL
                     IPAHVWTPWFAVLGSKSGFDSVEECYGDLAPHIFAVETGLSSDPPMNWRVSRLDRFTL
                     ISNSDAHSPPMLGREACAFATDLDYFAIRRALETGEGYRGTVEFFPEEGKYHLDGHRA
                     CNVRLEPAATRSCQGLCPACGKPLTVGVMHRVEELADRAGPIDGNTGRPRTDPFRSLV
                     PLPEILAEIRGVGAKSRSVEQAFSQVVARLGPELDILERLPLEEVARSGDPLLPEAIE
                     RLRAGKVRRESGYDGEYGVIRLFDPGEIRGRSVVAPLFGPHPSSGGPHPPTPSPASPP
                     PSPGEGEEGFCSRQNVGEQASVGGALLDGLDPEQRAAAEALDGPLLILAGPGTGKTRT
                     LTHRLAHLISERGVDPGRCLAVTFSRRAAAEMEERLAALVPAAAGHLVVATFHGLGLR
                     LLREFGLERDVRVALPAEREEIVRELFDISPSRAGRLLEEMGEVRRRQGIDPAAEVEC
                     PPELAGRIVLYEEALERRSLLDFDDLLLRPLRSLRERPDLAAACRERFGWISIDEYQD
                     IDPLQYSLVRQLAPPTGNVCAIGDPDQSIYGFRGADVGFFLRFREDFPGARLVQLTRN
                     YRSTHRVVEAACQAIRPGSLVPGRRLVAVRDGQGEPLVVQRAASERAEAELVVHTLER
                     LLGGTSSFSFDSGRVGYGDAPALSFGDVAILYRTDTQAAPLAEALGRAGIPFQKRSHR
                     PLSEQPGVRELLSAMEALREGSGPGGVEALLDRAAGSDKGAVADLLRPLAIRCGEDLD
                     GFLAEVALGGEVDTWDPRAERVSLLTLHAAKGL"
BASE COUNT          984 a         2426 c         2413 g         1078 t
ORIGIN      
        1 taccaccacc tcatggagca ctgggactgg cgggacaccg gcatcccacg cgccgccctt
       61 gcggatatca tgcgctccaa accggatgga gcccgtcatg aagagcctcg ttctcgccct
      121 gtgcctggcc atggcgtccc ccatggcggg gcagccacgg ctgctgacgc ccgacgacgt
      181 ctatcacctg aaaaccgtaa gagagcccca ggtcagcccc gacgggcggt gggtggccta
      241 caccgtggag cggctcgacc gggatgagga caccctcgac cgcgacgtct ggatggtgcc
      301 gtatgccggc ggcgagccgg tgcgcctcac ggcgagcccc aagagcgagc gcagcccccg
      361 cttctcgccg gacggccgct atctcgcctt cctctcctcc cgcgaggggg agaagagcca
      421 ggtctggctc ctcgaccgcc ggggcggcga cgccgtccgc ctgaccgact acaaggcgga
      481 cgtcgccgag ctcgcctggt cgcccgacgg caagcgcctg gccctggtgg tctccgacgt
      541 cgacccggac gaggagcagg acgaggggga ggacggggag gagaaggagg acaggcccaa
      601 gccgatcgtc atccggcgcc tccaattcaa gcgcgacggc gagggctacc tgcgggagat
      661 ccgcagccac ctctacgttt tcgacgtcac gtccaagacc tcgacccagg tcacctcggg
      721 tccctatgac cagagcgagc cggtctggtc gccggacggg cagtggatcg ccttcaccag
      781 caaccggtcc acgccggacc cggacgccaa cgagaacacc gacgttttcg tggtcgccgc
      841 caaggccggc gcgacgccgc ggccggtgac cacctccccc ggcggcgatt cccaacccgc
      901 cttcagcccc gacggccggc ttctcgccta cgtggccggc ggcgaccctg gcgatctctg
      961 gtacggcgcg agccacgtgg ccgtggtgcc ggtggccggg ggaccttcca agcccctgac
     1021 gcagtcgctg gaccgcaacg tccagtcccc gctcttctcc ccggacggcc ggcacgtcct
     1081 cttcctggtg gaggatggcg gcaaccagca cgtggcccgg gtgccggtgg cgggcggcgc
     1141 ggtggagcgg gtggtggccg gagagcgaga ggtggaggcc ttcgacgtcg ggccgaaggg
     1201 ggagctcgcg gtgctcgagt cgtcgcccca ccagccggcc gagatctcgg ctgccgaaac
     1261 ggctgcggtc ggcggagcgc cgcgtcgcct cacccgggtc aacgacgcct ttctcgccgg
     1321 catccgactg ggctcggtgc ggcgctacaa ggcgaagagc gccgacggca ccccagtcga
     1381 cgccttcctc actctgccac cggacgcgcc gcagggccgg cgcctgccga cggtgctgcg
     1441 catccatggc gggccgacgg cgcagtacag caccggcttc aagctggagt ggcagatcct
     1501 ggccgcccgc ggctatgccg tgatcggctc gaacccccgc ggctcgaccg gctacggccg
     1561 cgacttctcc cgcgccatct gggcggactg ggggaacaag gatttccagg acgtcatggc
     1621 ggcggtggac gaagcggtcg ccatgggcgt cgccgatccg gaccggctag gggtgggggg
     1681 ctggagctac ggcggcatcc tcaccaacta cgtgatcgtg cagactgacc gcttcaaggc
     1741 ggcgatctcg ggggcgagcg aggtcaacta cctctcgaac tacggcaccg accactacca
     1801 gtacgcctgg gagaccgagc tcgggctgcc gtggaggaac accgagctct ggatccgcct
     1861 ctccccctgg ttccgggtgg agaaagcggt gacgcccacc ctcttcgtct gtagcgggga
     1921 cgacgtcaac gtcccctgcc tcaactccga gcagttctac caggccatgc gccgcctggg
     1981 ccgcgacacc gagctggtca tctaccccgg ccagacccac gcggtccgcc gccccagctt
     2041 ccagaaggac cgcttcgagc gctacatcgc ctggtacgac aagtacctga agcccgaggg
     2101 ggcgaaggcg gcggccggga gatagcccat gaacagcctg gtcctgctgg tcattcgacg
     2161 agcgggccgt cgcgggcgat ccgccggagt ccgggaaggg gaactgagcg gcggcggttt
     2221 acaatcccgt catggatgat ctgatcgcca tactcatcgc cgccgccttc ttcgtcgtct
     2281 tctatctgtt cgtcaaagcc aaggagcggc gggagaggga cccgggcatc agcctcttcg
     2341 gcggggagcc gaccgacccc ccgcctcctg aagagcgtca atagttcttg gcgaagatcg
     2401 ccgtctcctt cttctccccg ccacagacca cgcagcggtc gaagccgccg tccggcacct
     2461 cgtcgagagg gatgcagcgg atggtgactt tgagctgctc cttcaccttc gtctcgcagt
     2521 cggcgctgcc gcaccagggg gagacggcga agccgccgtg gatctcgggt ttgtcggcgt
     2581 tcttcgcggt gaagaagccg gtgaactcgt tccagtcgcc gatcggccgc atgtgctgcg
     2641 cccggaactc cttcgccttc gccagcagcc ccgcctggat ctcctccagc acccgcgcca
     2701 ccgtcgccgc cagctcggcc cggggcaccg cctgcgtctg gttgaccgcc tggtcgcgcc
     2761 gcgcgaagga gacgacgccg ctgtcgacgt cgcgggggcc gatctcgagg cggatgggga
     2821 tccccttctt gacccagtcc cacttcttct ggccgccgcg gatgtcgcgg ctgtccacct
     2881 ccacctccac cggccggttg ccgtagaagg tgcggcgcag ctcggcggcg atctcggcga
     2941 cgtagtccag caccttctgc cgggagtcgt cgcgcaggat cggcaacacg accacgtgcg
     3001 cgggcgccac ccgcggcggc accacgaagc cgttgtcgtc gctgtgggtc atgatgacgc
     3061 cgccgatcat gcgggtggac gtcccccagg aggtggtcca ggcgtactcc tcggtgccgg
     3121 cctgcgactg gaacttgatg ccgctcgcct tggcgaagtt ctggcccagg aagtgggagg
     3181 tcccggcctg cagcgccttg cggtcctgca tcatcgcctc gatggtgaag gtctcgtcgg
     3241 cgccggggaa gcgctcgctc tcggtcttgc ggccgcggat caccggcacg gcgaggtagt
     3301 cctctgagaa gtcgcggtag acgtcgagca tcttccgggt ctcctccatc gcctcctcgg
     3361 cggtctcatg ggcggtgtgc ccctcctgcc agaggaactc ggcggtgcgc aggaacatcc
     3421 gggtgcgcag ctcccaacgc accacgttgc accactggtt gatcagcagc ggcaggtcgc
     3481 ggtagctctg cacccacttg gcgaacatct cgccgatgat cgtctcggag gtgggccgga
     3541 cgatgagcgg ctcctcgagc ctggcctccg gatcgaccac cagctcgccc ttctcgtcct
     3601 ggatgagccg gtagtgggtc accaccgcgc actccttggc gaagccctgg acgtgctcgg
     3661 cctccttctc caggaaggac ttggggatga acagcgggaa gtaggcgttg cggtgccccg
     3721 tcgccttgaa catcgcgtcc atctcgcgct ggatgttctc ccacagggtg tagccccagg
     3781 gcttgatgac catgcagccg cggaccggcg agctctcggc caggtcggcg gcgcggatga
     3841 tctgctggta ccactcggcg tagtcctcgg cgcgagtggg ggtgatcgca gtggtttctt
     3901 cttttgccat gttcggggtt ccggcctgac ggaggtccgg gccgcgcatc atatcccaga
     3961 ggagagtggg ggcaaagttc tctccaggca aaccgcgcgc ggcgtctgga cagaatcgaa
     4021 atgacggagt atcgtcatcg cgatctctct ggacgcttca gtggtcttct acgccgatct
     4081 ccacgtccac tcccggtact cccgggccac gagccgcgac gcggacctcg agcacctggc
     4141 gctgtgggcg cggcggaagg ggatcaccgt cgtggggacc ggcgacttca cccatccggc
     4201 gtggttggag gagatccgga cgaagctggt ccctgccgag cccgggctgt tccggctccg
     4261 ccccgagatc gagcgcgagg tggagcggga gctgccgccg tcctgccgcg ggccggtccg
     4321 cttcctcctc gaggtggaga tctccaccat ctacaagaag ggggaccgca cccggaaggt
     4381 ccatcatctg ctctatgcct ccaacctgga gacggtcgcc cgcatgcggg agaggctcgg
     4441 cgccatcggc aacctcaacg ccgacggccg ccccatcctg ggcctcgact cccggcacct
     4501 gctggagatc accctggaga gcgggccgga cgcctacctg atcccggcgc acgtctggac
     4561 cccctggttc gccgtcctgg ggtcgaagtc cggcttcgac tcggtggagg agtgctacgg
     4621 ggatctcgcg ccgcacatct tcgccgtgga gacggggctc tcctccgatc cgccgatgaa
     4681 ctggcgggtc tcccgtctcg accgcttcac cctgatcagc aactcggacg cccactcgcc
     4741 ccccatgctg gggcgcgagg cgtgcgcctt cgccacggac ctggactact tcgccatccg
     4801 ccgggcgctg gagaccggcg aggggtaccg cgggacggtc gagttcttcc cggaggaggg
     4861 caagtaccac ctggacggcc accgcgcctg caacgtgcgg ctggagccgg ccgcgacgcg
     4921 gagctgccag gggctctgtc cggcgtgcgg caagccgttg accgtcgggg tgatgcaccg
     4981 ggtggaggag ctggcggacc gggccgggcc aatcgatgga aacaccggcc ggccgcggac
     5041 ggacccgttc cgctccctgg tgccgttgcc ggagatcctg gcggagatcc gcggcgtggg
     5101 cgccaagtcg cgctcggtgg agcaggcctt ctcgcaggtg gtggcccggc tcggcccgga
     5161 gctcgacatc ctggagcggc tcccgctcga agaggtggcc cgaagcgggg acccgctgct
     5221 gccggaggcg atcgagcgac tgcgggcggg caaggtccgc cgcgagtccg ggtacgacgg
     5281 cgagtacggg gtgatccggt tgttcgatcc cggtgagatc agggggcggt cggtggtggc
     5341 gccgctgttc ggccctcacc cgtcgtccgg aggccctcac cccccgaccc cctctcccgc
     5401 ctccccgccc ccctccccgg gagaggggga ggagggcttc tgctcaagac agaatgtagg
     5461 ggagcaggca tccgtgggcg gtgccctcct cgacggtctc gaccccgagc agcgggccgc
     5521 ggcggaggcc ctggacgggc ctctcctcat cctcgcgggg ccgggtacgg ggaagacccg
     5581 gacgctcacc caccgtctgg cccatctgat ctccgaacgc ggcgtcgatc ccgggcggtg
     5641 cctggccgtc acgttcagcc gccgggccgc cgcggagatg gaggagcgcc tggcggctct
     5701 cgtgcccgcc gcggccgggc acctcgtcgt cgccacgttc catggcctgg ggctccggct
     5761 gctgcgcgaa ttcggccttg agcgggacgt ccgtgtcgcc ctgcccgccg agcgggagga
     5821 gatcgtccgc gagctgttcg acatctctcc ttccagggcc ggcaggctcc tggaggagat
     5881 gggggaggtc cggcgccgcc aggggatcga tcccgccgcg gaggtggagt gcccgccgga
     5941 gctggccggc cggatcgtcc tctacgagga ggccctggag cgccggagcc tgctggactt
     6001 cgacgacctg ctgctccggc cgctgagatc cctccgggag cggcccgacc tggcggcggc
     6061 ctgccgggag cgcttcggct ggatctcgat cgacgagtac caggacatcg acccgctcca
     6121 gtattccctc gtccgccagc tcgcgcctcc caccggcaac gtctgcgcca tcggcgaccc
     6181 ggaccagtcc atctatggct tccggggtgc ggacgtcggt ttcttcctcc gcttccggga
     6241 ggacttcccc ggcgcccgcc tggtccaact cacgcggaac tatcgctcga cccaccgggt
     6301 ggtggaggcg gcctgccagg cgatccgtcc ggggagcctg gtgccggggc ggcggctggt
     6361 ggcggtccgg gatgggcagg gggagcctct ggtcgtgcag cgggcggcga gcgagagggc
     6421 cgaggccgag ctggtggtcc acactctgga aaggctcctg ggcggcacct cctccttctc
     6481 cttcgacagc ggccgggtgg gctacggcga cgcgcccgcc ctgtcattcg gcgacgtggc
     6541 catcctctac cggaccgata cccaggcggc gcccctggcc gaagccctgg gccgcgccgg
     6601 catccccttc cagaagcgct cccaccggcc gctgtccgag cagccggggg tgcgcgagct
     6661 gctttcggcc atggaagcgc tccgggaggg ctcggggccg ggcggggtgg aggccctcct
     6721 cgatcgggcg gcaggttcgg ataagggagc cgtggccgac ctgctccggc cgctggcgat
     6781 ccgctgcggg gaggatctgg acggtttcct ggccgaagtg gcgctgggtg gagaggtgga
     6841 cacctgggac ccgcgggcgg agcgggtctc gctgctcacc ctccacgcgg ccaagggtct
     6901 g
//
LOCUS       DASPJI010000078         4479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_294075, whole genome shotgun sequence.
ACCESSION   DASPJI010000078 DASPJI010000000
VERSION     DASPJI010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294075"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VEL74_02100"
     CDS             complement(<1..514)
                     /locus_tag="VEL74_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61348.1"
                     /translation="MASENLTGFRLSPQQEHLWRVWQGGGGTYQAECRVRLTGAVDTP
                     ALSRALEDVVLRHEVLRTVLRPVPGLALPLQVVLDAAAEPLAPFDGDGRSPLRARLVP
                     DAPGSTDGLRLLLDLPALCADSISLENLVREIALAYAARRGTPVERDEPIQYVDVSEI
                     FHDFLSEEDPT"
     gene            complement(504..>4479)
                     /locus_tag="VEL74_02105"
     CDS             complement(504..>4479)
                     /locus_tag="VEL74_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61349.1"
                     /translation="SAALTRLARAQGGTLFMVLLAGFAALLSRWTGQEDIAVGSTIAN
                     RNRAEIEGLIGFFVNSLVLRTDCAADPDFVQLLDRVRKLSLAAFAHQDLPFEKLVEEF
                     AVERDPSRTPLFQVLFALQNASFTALSIPGLTFSPIETQGTTAKLDLTLSVAEAPEGL
                     YASWEYNRDLFDTATVARMAGHLEVLLGGVLAAPDRRLSELPLLSTGELSQLLREWND
                     TEVPCPQLPLVHELFAAHAQRRPGAAALTWSGGCLTYGELDRRAGRLARHLRSLGVGP
                     EVLVALCTERTSPERVVGIVAVLKAGGAYVSLDPAYPQERLAFLLADSRAPVVLTQER
                     LAGLLPASPAAVIHLDGDPGAFGPEAEDALPDSGVAPDNLAYVVYTSGSTGRPKGVEI
                     PHSGLMNLVRWHQTLYRVGPEDRGTQVASPAFDASIWELWPYLAAGASLHIPDDETRL
                     SSSGMLRWWTQEGITLAYLMTPLAEGVLEEEIPPSLDLVVRALIIGGDRLRRGPRPEA
                     GFRLMNHYGPAEYTVTSTVVEVPPEGERRGIPTIGRPIDNTRIHVLDRDFNPVPIGVL
                     GELFVAGIGLARGYLGRPDLTAEKFVPDPFAGPEKPGARIYRTGDLVRYLPDGDIDFQ
                     GRLDHQVKLRGLRIELGEIEARLAQHPQVREATVLVREDRPGDRRLVAYWVPADEPPA
                     DDELRDFLRERLPAYMVPAAFIVLPALPLTPNGKVDRKALPAPERQSTEETWRAPRTQ
                     VEEILAGIWAELLGLERVGTADSFFELGGHSLLATQVMSRLRSAFDVEIPLRDVFEAP
                     ILSDFAARIETLRRTGAVPSAPPLVPAPRGGPLPLSFAQRRLWFIDQLEPGSPLYNIP
                     VALRVEGPLHPGVLELCLGEIVRRHEALRTTFALIGDEPAQVIAPSRSVALPVIDLQA
                     VPDPARTAELERLAREEGEIPFNLARGPLLRVTLLPLEPGASALLLTLHHVISDGWSM
                     RVLIRDLVELYRTAGRPSPLPELPVQVADHAVWQRAWLRGDVLAAQLADWRRRLTGAP
                     TVLELPTDRPRPAVTSQRGRRVYRLLPGALAERLRELGRHEGATLFMTLLAAFDVLLA
                     QRSGRRDLLVGTPIAGRSHPEVEELIGCFVNLLPLRAEMSGDPSFRELLQQVRATTLE
                     ASLHQDLPFEALVDELGLERGLAHHPLVQVAFTFDEPRLETLEVPGMAVHPLRSEGGE
                     TRAQFDLTLNAWLREEGLGTSLTYSTDLFRPASAERLLDDFEVVLRQVAEQPALRLSE
                     LIDELTRVDRERRTASQEEMGSVRAEKFKRLRRRGVPAPSGEPTLAGTHAASGARQEA
                     EHGE"
BASE COUNT          698 a         1556 c         1616 g          609 t
ORIGIN      
        1 cggtcggatc ttcctcgctc aggaagtcgt ggaagatctc ggagacgtcc acgtactgga
       61 tcggctcgtc gcgctcgacc ggggtgccgc ggcgggcggc gtaggccagc gcgatctcac
      121 gcaccaggtt ttcgaggctg atcgagtcgg cgcagagggc agggaggtcg agaagcaggc
      181 ggaggccgtc cgtggagcca ggggcgtccg ggacgagccg cgcgcgcagt ggtgaccggc
      241 cgtcgccgtc gaagggcgcg agcggttccg ccgccgcgtc gagcacgacc tggagcggca
      301 gggcgagacc cgggaccggg cgcaggaccg tgcggagcac ctcgtgtcgc agcacgacgt
      361 cctcgagggc gcgggatagg gccggagtgt ctaccgcgcc ggtgaggcgg accctgcact
      421 ccgcctgata ggtgccgccg ccgccctgcc agacccgcca caggtgttcc tgctgagggg
      481 agagccggaa gccggtcagg ttctcactcg ccatgctcgg cctcctgccg tgctccagag
      541 gcggcgtggg taccggcgag agtcggctcc ccggacggcg ccgggacgcc ccgccggcgc
      601 agccgcttga acttctcggc gcgcacgctg cccatctcct cctggctggc ggtccgccgc
      661 tcccggtcca ctcgggtgag ctcgtcgatg agctcgctca gccgcagggc cggctgctcg
      721 gcgacctggc gcaacaccac ctcgaagtcg tcgagtagcc gctcggcgct ggccggcctg
      781 aacaggtcgg tgctgtaggt gagggaggtc cccagccctt cctcccgcag ccaggcgttc
      841 agcgtcagat cgaactgcgc ccgggtctcg cccccttcgg atcgcagcgg gtggacggcc
      901 atcccgggta cctccagggt ctccaggcgg ggctcgtcga aggtgaaggc gacctggacg
      961 agcgggtggt gcgccaggcc gcgctccagc ccgagctcgt caaccagggc ctcgaagggg
     1021 agatcctgat gcagggacgc ctcgagagtg gtggcgcgaa cctgctgcag cagctcgcgg
     1081 aagctcgggt caccggacat ctcggcgcgc agggggagca ggttcacaaa gcacccgatg
     1141 agctcctcga cctccggatg actgcgcccg gcgatggggg tgcccaccag caggtcgcgc
     1201 cggccggagc gttgcgcgag caggacatcg aaggcggcga ggagagtcat gaacagggtc
     1261 gccccttcat gccggccgag ctcgcgcagc cgctccgcca acgcgccggg gaggagacgg
     1321 tacacgcgcc gtccccgctg gctggtgacc gccggtcgcg gccgatcggt cggcagctcg
     1381 aggaccgtcg gggcgccggt gaggcggcgc cgccagtcgg cgagctgcgc ggcgagaacg
     1441 tcgccgcgca gccaggcgcg ctgccagacc gcatggtccg ccacctgcac cggcagctcc
     1501 gggagcggcg acggacgccc ggcggtccgg tagagctcca ccaggtcgcg gatcagcacc
     1561 ctcatcgacc agccgtcgct gatgacgtgg tgcagggtga gcagcagggc gctggctccc
     1621 ggctccaggg gaaggagggt gacgcgcagg agcggcccgc gtgcgaggtt gaaggggatc
     1681 tctccttcct cgcgcgccag acgctcgagc tccgcggtgc gcgcggggtc cggcaccgcc
     1741 tgcagatcga tcaccggcaa ggcgacggac ctcgacggcg cgatcacctg cgccggctcg
     1801 tccccgatga gggcgaaggt ggtgcgcaat gcctcgtgac ggcgcacgat ctcgccgagg
     1861 cacagctcca gcaccccggg gtgcagcggc ccttcgacac gtagagccac cggtatgttg
     1921 tatagcgggc tgcccggctc gagctggtcg atgaaccaga ggcgccgctg agcgaacgag
     1981 agagggaggg gcccgcccct gggggccggg accaggggcg gagccgacgg gaccgcgccg
     2041 gtgcgtcgta aggtctcgat ccgtgccgcg aagtccgaca agatcggggc ctcgaagaca
     2101 tcgcggagcg gtatctcgac gtcgaaggca ctccgcagac gcgacatcac ctgtgtcgcc
     2161 agcagcgaat gaccacccaa ctcgaagaac gagtcggccg tcccgacccg ctcgagaccc
     2221 aggagctcgg cccagatccc cgcgaggatc tcttcgacct gcgtgcgcgg cgcccgccag
     2281 gtctcctcgg tgctctgcct ttccggcgcc ggcagggcct tccggtccac cttgccgttc
     2341 ggggtgagcg gcagtgccgg gaggacgatg aacgccgccg gcaccatata ggcggggagt
     2401 cgctcgcgaa ggaagtcacg gagctcatcg tccgccggtg gctcgtccgc cggtacccaa
     2461 taagccacca gccggcggtc accgggacgg tcctcccgca ccagcacggt cgcctcccgc
     2521 acctgcgggt gctgggccag cctcgcctcg atctcgccca gctcgatccg cagaccgcgc
     2581 agcttcacct ggtggtccag acgtccctgg aagtcgatgt cgccgtccgg cagatagcgc
     2641 accagatcgc ccgtccggta gatccgggcc ccgggctttt cgggaccggc gaacgggtcc
     2701 ggcacgaact tctccgccgt caggtccggc ctccccaaat agcctcgcgc caagccgatg
     2761 ccagcgacga agagctcgcc caggacgccg atggggactg ggttgaagtc ccggtccagg
     2821 acatggatgc gcgtgttgtc gatggggcgg ccgatggtgg ggatcccccg gcgttcccct
     2881 tccggcggca cctccaccac cgtcgaggtg accgtgtatt cggccgggcc atagtggttc
     2941 atcagccgga agccggcctc cggccgcggg ccgcggcgca ggcggtcgcc gccgatgatc
     3001 agcgcccgca ccaccagatc cagcgacggc gggatctcct cttcgagcac tccttcggcc
     3061 aggggggtca tcagataggc cagggtgatc ccctcctgcg tccaccacct cagcatcccc
     3121 gaagacgaga ggcgggtctc gtcgtccggg atgtgcaggc tcgctcccgc cgccagatag
     3181 ggccagagct cccagattga ggcgtcgaac gccgggctcg ccacctgggt gccacggtct
     3241 tccgggccga cgcggtacag cgtctggtgc cagcgcacca ggttcatcag cccactgtga
     3301 gggatctcga cccccttggg ccggcccgtc gagcctgacg tgtagacaac gtaagccagg
     3361 ttgtcggggg cgacgccgct gtccggcagt gcgtcttcgg cctcggggcc aaacgccccg
     3421 ggatcgccgt cgaggtggat cacggcggcc ggcgaggccg gcaacagccc ggcgagccgc
     3481 tcctgggtca gcaccaccgg cgcccgcgag tcagcgagca ggaaggccag tctctcctgg
     3541 gggtaggccg gatcgagcga gacgtaggcg cctccagcct tcagcacggc gacgatcccc
     3601 accacccgct caggggaggt ccgctcggtg cacagggcca ccaggacctc gggcccgacg
     3661 ccgagcgacc gcagatgccg ggccagccgg ccggcccgcc ggtcgagctc gccgtaggtc
     3721 agacacccac ccgaccaagt cagggcggcg gccccggggc gccgttgggc gtgggcagcg
     3781 aacagctcgt ggaccagagg gagctgcggg caggggactt ccgtgtcatt ccactcccgg
     3841 agcagttgcg acagctcgcc ggtactcaac agcggcaact cggacaggcg gcggtcgggg
     3901 gccgccagca cgccgccgag caggacctcc aggtgcccgg ccatgcgcgc caccgtcgcg
     3961 gtatcgaaga ggtcccggtt gtactcccag gaggcgtaga ggccctccgg tgcctcggcg
     4021 actgacaggg tcaggtcgag cttcgccgtc gtcccttgcg tttcgattgg cgagaacgtc
     4081 aacccgggga tcgagagcgc cgtgaagctc gcgttctgga gcgcgaacag cacctggaac
     4141 agaggcgtac ggctagggtc gcgctccacg gcgaactctt cgaccagctt ctcgaagggc
     4201 aggtcctgat gcgcgaaggc cgcgagcgag agcttccgga ccctgtcgag gagctgcacg
     4261 aaatccggat ccgccgcgca gtcagtgcgc agcaccaggg agttgacgaa gaatccgatc
     4321 aggccttcga tctcggcccg gttgcggttg gcgatggtcg accccacggc gatgtcctcc
     4381 tggcccgtcc agcgggagag gagggccgcg aagcctgcca ggaggaccat gaagagggtg
     4441 ccgccctgag ccctggcgag cctggtgaga gcggcgctc
//
LOCUS       DASPJI010000079         3551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_465666, whole genome shotgun sequence.
ACCESSION   DASPJI010000079 DASPJI010000000
VERSION     DASPJI010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465666"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VEL74_02110"
     CDS             complement(<1..687)
                     /locus_tag="VEL74_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61350.1"
                     /translation="MRRLSSALLASVSVVAFTLLLAGPLAAYTVVLKDGKTIIAKEKY
                     RIENGKAIIILPSGERTAMDAREIDVRRTEEANRNNYGTAIVLEQSTKPSAPPPPPPP
                     RQKSLSDLIANRDAAPRELPEARREGSAARAPGTSGSAGGGAARTRAGFVDLSTVSRR
                     PYPHLEVASELQQFFRGQGLEEVGIYSGTQGDRPLVEVTTNSEASVFRALAIGSNALL
                     HIRDRHPQRVE"
     gene            790..1872
                     /gene="carA"
                     /locus_tag="VEL74_02115"
     CDS             790..1872
                     /gene="carA"
                     /locus_tag="VEL74_02115"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HYG61351.1"
                     /translation="MPQPALLLLEDGSVFEGEAVAPGTRFGEVVFNTSMTGYQEVLTD
                     PSYRGQIVVMTQPHIGNYGTTAEVAESRRPWVEGFIARQFTVRPSGSGDESLVTYLQQ
                     HGVPALQGIDTRALVRRLRERGALRGVLTSERSDVDALAAELADFPTMTGRALVDEVT
                     CAAPYEIAAVGEERRHLAVYDFGVKANILRELAERGTRLTVLPARTPASECLALPVDG
                     VVLSNGPGDPEPLTGIIESVRELVAAEIPVLGICLGHQLLGLAMGGQTFKLKFGHHGG
                     NQPVRDLATGKVAITSQNHGFAVRPDSLPAGCAVTQLNLNDSTVEGFAVTGKPVFSVQ
                     YHPEAAPGPHDASELFDRFLAVAARR"
     gene            1869..3125
                     /locus_tag="VEL74_02120"
     CDS             1869..3125
                     /locus_tag="VEL74_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYG61352.1"
                     /translation="MSSPAGSGSPSPAGPLAGPLTWVIAWRFLRGQRSRLLDGTARAA
                     LLATALGVTAMVIAMALMTGYREDLQGKLIRGNAAVIAYPMRADQGMLTPGKRALLEK
                     IPGVEAIGIVAYGQGALSSGARPDGVEVILRGVDPGGGQLAASAEQLRSIDGIPGTVL
                     GADLAERLKVQQGEILRLVALGFEEGRPRFLYQSLRVNGTFSAGFAEFDRSWVLLDRG
                     MVERLMGGETAVDLVEFTVADPAEAPRIAEEATRVLEPDFVVTDWQQLNRELFTALKL
                     QQVALFLVLGLIVLVSTFNVASTLVVLVRERMRDIGLLGALGLPPGRVRLLFLLYGGL
                     LGGLGTLLGVGLGWGICWVLTTFELIRFDSEVAAIYFIDSVPFRVEPLDILAIVGFAL
                     AITLLACLVPAWRAARVDPSAALRYE"
     gene            complement(3144..>3551)
                     /locus_tag="VEL74_02125"
     CDS             complement(3144..>3551)
                     /locus_tag="VEL74_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYG61353.1"
                     /translation="SREVRFPAGASLFQEGDAGAEMYVILEGRVMISKFIPGAGEEAL
                     AILDRGDFFGEMSLIDGEPRSADARAYGGPLTVLALDQATVREVLAMDPHAALEFLQL
                     LCRLVANRLREIDEKVIGWRIMSGERNDESASA"
BASE COUNT          468 a         1290 c         1245 g          548 t
ORIGIN      
        1 ctccacccgc tgcggatgcc ggtcacggat gtggagcagg gcattggagc cgatggcgag
       61 ggcccggaag accgaggcct cggagttcgt ggtgacctcc accagaggac ggtccccctg
      121 ggtgccgctg tagatcccca cctcctcgag gccctggccg cgaaagaact gctgcagctc
      181 ggaggcgacc tctaggtgcg gatagggcct gcggctcacc gtcgacaggt cgacgaagcc
      241 ggcgcgggtc cgggcggcgc ctccgccggc gctgccggag gtccctggcg ctcgggcagc
      301 ggagccctcg cgccgcgcct cgggcagctc gcggggcgcc gcgtcgcggt tggcgatcag
      361 gtccgaaagc gatttctgcc gaggcggcgg cggcggcggc ggggcggacg gtttcgtgct
      421 ctgctcgagg acgatcgccg tgccgtagtt gttgcgattg gcctcctcgg tgcggcggac
      481 gtcgatctcc cgggcgtcca tggccgtccg ctcgccactg ggaaggatga tgatcgcctt
      541 gccgttctcg atccggtact tctccttggc gatgatcgtc ttgccgtcct tgagcacgac
      601 ggtgtacgcc gcgagcgggc cggcgaggag cagggtgaac gcgacgacgg agacactggc
      661 gagcagggca gagctgagac gacgcatgat tcctccgggg tacagaagcg gcagtcttga
      721 acgccccggt atgataccct gccgcggtcg ccgccggaac tgcggcgccc cactgacttg
      781 cgagacccca tgccccagcc cgccctgctc ttgctcgagg acggcagcgt cttcgaaggg
      841 gaagccgtcg cccccggcac ccgcttcggc gaggtggtct tcaacacctc catgaccggc
      901 taccaggagg tgctcaccga cccttcgtac cgcggccaga tcgtggtcat gactcagccc
      961 cacatcggca actacggcac caccgccgaa gtggccgagt cgcggcgccc ctgggtcgag
     1021 ggcttcatcg cccgccagtt caccgtccgc ccctccggca gcggcgacga gagcctggtc
     1081 acctacctgc agcagcacgg cgtgccggcc ctccagggga tcgacacccg cgccctggtg
     1141 cggcgcctgc gcgagcgggg ggccctgcgc ggcgtcctga cctccgagcg ctccgacgtc
     1201 gacgccctgg ccgccgagct cgccgacttc ccgaccatga ccggccgcgc cctggtggac
     1261 gaggtgacct gcgccgcccc ctacgagatc gcggcggtgg gggaggagcg gcgccacctg
     1321 gcggtgtacg acttcggggt caaggctaac atcctgcgcg agctggccga gagggggacg
     1381 cgcctgaccg tgctccccgc ccgcaccccg gcttccgaat gcctggcgct gccggtcgat
     1441 ggcgtggtgc tctccaacgg ccccggcgac ccggagcccc tgaccggcat catcgagtcg
     1501 gttcgcgagc tggtggcggc ggagatcccg gtgctcggca tctgcctggg gcaccagctc
     1561 ctcgggctcg ccatgggcgg ccagaccttc aagctcaagt tcggccacca cggcggcaac
     1621 cagcccgtgc gcgacctggc caccggcaag gtcgccatca ccagccagaa ccacggcttc
     1681 gccgtccggc ccgactctct gccggcgggc tgcgccgtca cccagctcaa cctgaacgac
     1741 agcacggtgg aggggttcgc cgtcaccggc aagccggtct tctccgtcca ataccatccc
     1801 gaggcggcgc ccgggccgca cgacgcctcc gagctcttcg accgcttcct cgcggtcgcg
     1861 gcccgccggt gagcagcccg gcgggctccg gctcaccttc tcccgccggc ccgctggccg
     1921 ggcctctcac ctgggtcatc gcctggcgct tcctgcgggg acagcgcagc cgcctcctcg
     1981 acggtaccgc gcgggcggcc ctcctcgcca ccgcgctggg cgtcaccgcc atggtgatcg
     2041 ccatggcgct gatgaccggc taccgtgagg acctccaggg caagctcatc cgcggcaacg
     2101 ccgccgtcat cgcctatccc atgcgggcgg accaggggat gctgacgccc ggcaagcgcg
     2161 ccctgctcga aaagatcccc ggcgtcgagg cgatcgggat cgtcgcctat gggcagggag
     2221 cgctgtcgag tggcgcccgt cccgatggcg tcgaggtgat cctgcgcggc gtcgaccccg
     2281 gcggcgggca gctcgcggcg agcgccgagc agctccggtc catcgatggc atcccgggca
     2341 ccgtcctggg ggcagacctg gccgagcggc tgaaggtcca gcagggtgag atcctccgcc
     2401 tggtcgccct tggcttcgag gagggacggc cgcgtttcct ctaccagagc ctgcgcgtca
     2461 acggcacctt ctcggccggc ttcgccgagt tcgaccgcag ctgggtgctg ctggaccgcg
     2521 ggatggtgga gcggctcatg gggggggaga ccgccgtcga cctggtagag ttcacggtcg
     2581 ccgacccggc ggaggcgccg cgcatcgccg aggaggccac ccgcgtcctg gagcccgatt
     2641 tcgtcgtcac cgactggcag cagctcaacc gggagctgtt cacggccttg aagctccagc
     2701 aggtggcgct cttcctggtg ctgggcctga tcgtcctggt ctccaccttc aacgtcgcgt
     2761 ccaccctggt ggtgctggta cgggagcgga tgcgcgacat cggcttgctc ggcgccctgg
     2821 gcctgccgcc gggccgcgtt cggctcctct tcctgctcta cgggggcctg ctgggcggcc
     2881 tgggcacgct gctgggggtg ggcctgggct gggggatctg ctgggtgctc accaccttcg
     2941 agctgatccg cttcgattcc gaggtggcgg cgatctactt catcgactcg gtccccttcc
     3001 gggtcgagcc cctggacatc ctggcgatcg tcggcttcgc cctcgccatc accctgctgg
     3061 cctgtctcgt gcccgcctgg cgcgccgccc gggtcgaccc gtcggcggcg ctgcggtacg
     3121 aatgaggggg ctactgggag gggctaggcc gaggcgctct cgtcgttgcg ctcgccggac
     3181 atgatgcgcc agccgatgac cttctcgtcg atctcccgca ggcggttggc caccaggcgg
     3241 cagaggagct gaaggaactc gagggcggcg tgggggtcca tggccaggac ctcccggacc
     3301 gtggcctggt cgagggccag caccgtcagc ggcccgccgt aggcacgggc gtcggccgag
     3361 cgcggctcgc cgtcgatcag cgacatctcg ccgaagaagt cgccgcggtc gaggatcgcc
     3421 agggcctcct cgccggcgcc ggggatgaac ttgctgatca tcacccgccc ttccaggatg
     3481 acgtacatct ccgcccccgc gtccccctcc tggaacagcg aggcgccggc cgggaaacgc
     3541 acctcgcgcg a
//
LOCUS       DASPJI010000080         3524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_4540, whole genome shotgun sequence.
ACCESSION   DASPJI010000080 DASPJI010000000
VERSION     DASPJI010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4540"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VEL74_02130"
     CDS             <1..945
                     /locus_tag="VEL74_02130"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HYG61354.1"
                     /translation="GRLSPAGTYPTGGRGNGTEPDALRSQGSLVLSGNLLFAVNAGSN
                     EISVFSIGKKNLALVEKVSSGGVKPTTLAVMDDLLYVVNAGSGQITGFRVASDGQLPP
                     LANSTRSLTGGPAADPSKISFTPDGGALLVTEKGSSANLIDVFRVGNDGLTDGPFPTP
                     SVGTGPFGFEFDRRGYLIVSEAFGGAPGAGAASSYELAADSTPSVISASVGTGQTATC
                     WVVTTGPNVYVTNTMSGTISRYRIDRDGDLSLVQAVAGNTGGAPIDMALSREGNYLYA
                     VVDVTGTIAAFRVEDDGELTPVRVRVDLPPFAQGIMAW"
     gene            complement(948..1136)
                     /locus_tag="VEL74_02135"
     CDS             complement(948..1136)
                     /locus_tag="VEL74_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61355.1"
                     /translation="MNDNVFGSNTFLTMANRPGFGLWAILLCWWILVPWALVLHWKRR
                     RQRPAPEPESPPRTAPPA"
     gene            complement(1179..3335)
                     /gene="secA2"
                     /locus_tag="VEL74_02140"
     CDS             complement(1179..3335)
                     /gene="secA2"
                     /locus_tag="VEL74_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HYG61356.1"
                     /translation="MTSSELAAIASREAALAGTTDRELRDRAGDLRRQALAGASLDTL
                     LPDCFAVVRETARRVLGERPFDVQMLAGIALHQGKLVEMQTGEGKTLAAVAPVALNAL
                     TGRGVHVLTFNDYLARRDGEWMGPVYERLGLTVSSIQDGLSTAERQRAYGCEVTYLTV
                     KQAGFDLLRDGLCLEPGDQVHRPFHLALVDEADSILIDEARIPLVIAGSTGETGIDLD
                     RLAGIARRLVRGTDYDDDAQNVFLTERGAHRAEELLGCGNLFAGDHVRLLAELRNALH
                     AEALLRRDVDYIVRGGRIELVDELTGRVAENRHWPGGLQAAIEAKEGLHRQPEGKIFG
                     SITIQHFLRRYPRLCGMTATASSSAAELAEVYGLDIATVPPNRPCVRVDHPDVIHVSK
                     EAKRRALVAEIASTHAAGRPVLVGTVSVEESEDLAADLHKAGIACRVLNARDDAEEAA
                     IVAEAGALGAVTISTNMAGRGTDIRLGGADEKQREAVVALGGLYVIGTNRHESRRIDD
                     QLRGRAGRQGDPGSSRFIVSLEDSLLQRSGIERLVPRRVLEGERAAGETGRTIDHPAI
                     RREVERVQRIVEGRHGDLRQRLLGYWQVLEHQRRQLQEWRQPVLEGRLEESLLARRSP
                     DRWSKLRETHGEELLAAIEQRITLVTIDRCWSDHLAEMQAVRDEIHLVQLGGQDPFAE
                     FCRAAGAAFETLPERIDETIVEFFERVEITADGVDW"
     gene            complement(3346..>3524)
                     /locus_tag="VEL74_02145"
     CDS             complement(3346..>3524)
                     /locus_tag="VEL74_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012180593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HYG61357.1"
                     /translation="GVDLRGYYVWSLLDNFEWSLGYSKRFGIVHVDYATQRRTPKASA
                     RYYSEVIRNHGLVP"
BASE COUNT          542 a         1208 c         1207 g          567 t
ORIGIN      
        1 ggaaggctct cgccggccgg cacctacccg acgggcgggc gcggcaatgg cacggagccg
       61 gacgcgctgc ggtcccaggg cagtctggtg ctcagcggca acctgctgtt cgcggtcaat
      121 gccggcagca acgagatctc cgtcttctcg atcggcaaga agaacctggc cctggtggaa
      181 aaggtctcct ccggcggtgt aaagcctacg accctggccg tcatggatga cctcctctac
      241 gtcgtcaacg ccggcagcgg acagatcact ggcttccggg tcgcctcgga cgggcaactg
      301 ccccccctcg ccaactccac cagatccctg accggcggac cggctgccga cccttcgaag
      361 atcagcttca ctcccgacgg cggagcgctg ctcgtcacgg agaaaggctc gagcgcgaac
      421 ctcattgacg tcttccgggt cgggaatgac ggtctcactg acggaccctt cccgacgcct
      481 tccgtcggga cgggaccctt cggcttcgaa ttcgaccggc gcggctatct gatcgtttct
      541 gaagccttcg gcggcgcccc cggtgccggt gccgcttcgt cgtacgagct cgccgccgac
      601 agcaccccta gcgtgatcag cgcttcggtc ggcaccgggc agacggcgac ctgctgggtg
      661 gtgacgaccg gtcccaacgt gtacgtgacg aacaccatga gcggcacgat ttcgcggtac
      721 cgcatcgaca gagatgggga cctcagcctg gtgcaagccg tcgcggggaa caccggcggc
      781 gctccgatcg acatggctct ctccagagag ggtaattacc tctacgccgt cgtcgacgtg
      841 acgggtacga tcgccgcctt ccgcgtggag gatgacgggg agctgacgcc cgtccgggta
      901 cgcgtcgact tgccgccctt cgcgcagggc atcatggcct ggtaggtcta ggccggaggc
      961 gcggtccgcg gcgggctctc gggctccggc gcgggccgct gccgccggcg tttccagtgc
     1021 aggaccagcg cccaggggac caggatccac cagcagagga ggatggccca caggccgaag
     1081 ccgggacggt tggccatggt gaggaaggtg ttgctgccga aaacgttgtc gttcaccaga
     1141 taggtccagg tggaggcggg gccccgcagg ccttcacgct accaatcgac cccgtccgcc
     1201 gtgatctcca cccgctcgaa gaactcgacg atcgtctcgt cgatgcgctc cggaagggtt
     1261 tcgaacgccg cccccgccgc gcgacagaat tcagcgaacg ggtcttggcc gccgagctgg
     1321 acgaggtgga tctcgtcgcg cacggcctgc atctcggcca ggtgatcgct ccagcaacga
     1381 tcgatggtga ccagcgtgat ccgttgctcg atcgccgcga gcagctcctc gccatgggtt
     1441 tcccggagct ttgaccagcg gtccgggctg cgccgggcga gcaggctctc ctccaggcgg
     1501 ccctccagca ccggctgccg ccactcctgc aattgacggc gctggtgctc gagtacctgc
     1561 caataaccga gcagccgctg gcgcagatcg ccgtgccggc cttcgacgat gcgctgtacg
     1621 cgctccacct cgcgccggat cgcaggatga tcgatcgtcc tgccagtctc gcccgccgct
     1681 ctctctcctt cgagcactct ccgcggcacc agtcgttcga tgccggagcg ttgcagcagg
     1741 ctgtcctcca ggctgacgat gaagcgggac gagccgggat cgccctggcg gccggcacgg
     1801 ccgcgcaatt gatcatcgat gcgccggctc tcgtggcggt tggtgccgat cacgtacagc
     1861 ccgccgaggg ccacgaccgc ttcgcgctgc ttctcgtccg cgccgccgag gcggatgtcg
     1921 gtgccgcggc cggccatatt ggtggagatc gtcaccgcgc ccagggctcc ggcctcggcc
     1981 acgatcgcgg cctcctcggc gtcgtccctg gcattgagca cccggcaggc gatcccggcc
     2041 ttgtgaagat cggcggcgag gtcctccgat tcctcgacgc tgaccgtccc aaccagcacc
     2101 ggccggcccg cggcatgggt gcttgcgatt tccgcaacca gggcgcgccg ctttgcctcc
     2161 ttcgagacat ggatcacgtc gggatgatcg acgcggacgc acggtcggtt cggcggcacc
     2221 gtcgcgatgt cgaggccgta gacctcggcg agctcggcgg cggaggagct ggcggtggcc
     2281 gtcatgccac agaggcgcgg gtatcggcgc aggaagtgct ggatggtgat cgaaccgaaa
     2341 atctttcctt cgggttgccg gtgcagcccc tccttcgcct cgatcgccgc ctgcaggcca
     2401 ccgggccagt ggcggttctc ggcgacccgg ccggtcaatt cgtccaccag ctcgatgcgg
     2461 ccgccgcgga cgatgtagtc cacgtcacgc cgcagcaggg cctcggcgtg cagggcgttg
     2521 cgcagctccg ccagaagccg cacgtggtca ccggcgaaaa ggttgccaca acccagcagc
     2581 tcttcggcgc ggtgcgctcc gcgctcggtg aggaagacgt tctgcgcgtc gtcgtcgtag
     2641 tcggtcccgc gcacgagccg gcgggcgatc ccggccagac gatcgagatc gatccccgtc
     2701 tcgccggtgc tgccggcgat caccaggggg atgcgcgcct cgtcgatcag gatcgaatcg
     2761 gcctcgtcca ccaacgcgag atgaaacggc cggtgcacct gatcgccggg ctccaggcag
     2821 aggccgtcgc gcaggaggtc gaagccggcc tgcttgaccg tcagataggt gacttcgcag
     2881 ccgtaggccc gctgccgctc cgcggtggag agcccgtcct ggatcgagga gacggtgagg
     2941 ccgagccgct cgtagacggg gcccatccac tccccgtcgc ggcgggccag atagtcgttg
     3001 aaggtgagga cgtgcacgcc gcggccggtg agggcgttga gcgccacagg ggcgacggcg
     3061 gcgagtgtct tgccctcgcc ggtttgcatc tccaccagct tcccctggtg cagggcgatg
     3121 ccggcgagca tctggacatc gaaggggcgc tcgcccaata cccggcgggc ggtctcgcgc
     3181 acgacggcga agcagtccgg gagcagggta tcgagggagg cgccggcgag ggcttggcgg
     3241 cgcaggtccc ccgcacggtc gcgcagctcc cggtccgtgg tcccggcgag agccgcttcc
     3301 cgggaagcga tggctgcgag ctcggagctc gtcaaggatc gggcctcatg gaaccagacc
     3361 gtgattccgg atgacctccg agtagtagcg ggcgctggcc ttcggggtgc ggcgctgggt
     3421 ggcgtagtcc acgtggacga tgccgaagcg cttcgagtag ccgaggctcc attcgaaatt
     3481 gtccagcaag gaccagacgt agtagccgcg caggtccacc ccct
//
LOCUS       DASPJI010000081         6486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_294309, whole genome shotgun sequence.
ACCESSION   DASPJI010000081 DASPJI010000000
VERSION     DASPJI010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294309"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VEL74_02150"
     CDS             <1..522
                     /locus_tag="VEL74_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HYG61358.1"
                     /translation="PVYAESEEDTLEGVVGMLLKAAGATLTVAESCTGGLLGERLTRV
                     PGSSDYFLGGAIVYTNRLKTELAGVPPELLQEHGAVSEPVARAMAEGVCRALGSDYGI
                     AVTGVAGPGGGSEAKPVGTVHIAVAGPEGTSHRRVRFPGDRQRVRQHSSQLALDMLRR
                     KLLGLDPQGMGWR"
     gene            519..1103
                     /gene="thpR"
                     /locus_tag="VEL74_02155"
     CDS             519..1103
                     /gene="thpR"
                     /locus_tag="VEL74_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HYG61359.1"
                     /translation="MRLFVAVEVPEAVRREVARRVASVRDRLPQARWVDPEMIHLTMV
                     FLGEVPEDRVPALAEALRQAFAPYPPLPMRLSGAGTFPPGRPARVAWLGLDAPDDLTS
                     LQGDAVRAAVAAVEHEPEDRPYRAHVTLARCQSPWRRDAVDKFIAAFPGETGQPWVAD
                     RGVLMESKLSPKGARYRVVEAFPLEGPSLVTGDE"
     gene            1100..1732
                     /gene="plsY"
                     /locus_tag="VEL74_02160"
     CDS             1100..1732
                     /gene="plsY"
                     /locus_tag="VEL74_02160"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HYG61360.1"
                     /translation="MSTGTALAIIAAAYLVGSIPWSYLIVRLRQGMDIRAVGSGNVGA
                     TNVLRTAGKGAGIVALLLDAGKGVAVVLVTRALDAPAYVVCVSAVAVVLGHVFPVFLR
                     FRGGKGVATAAGALGALAPVVLALSLLGFVVIVLWKRYVSLGSILAAALFPVFAWIGS
                     RLGWVRHEGAWVVLSSAAIAAVVLARHASNLRRLLKGTERRLGEPRSIRQ"
     gene            1757..2785
                     /locus_tag="VEL74_02165"
     CDS             1757..2785
                     /locus_tag="VEL74_02165"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HYG61361.1"
                     /translation="MKQIAVLGAGSFGTALAVHLASMGANAGGNRVRLWARNPELARQ
                     MAEERANPEYLPDVEIPAGVEPTAEMEPLAECDIVLVVVPSHGFRGVLRQFLRAHPAD
                     RPAVIVSATKGIETDTLARMSQVSFEEGTAAGREVRFATLSGPTFAAELARNSPSAAV
                     VASEDAALATEIRERLSTRLLRLYSSSDVVGVELGGTVKNVVAIAAGTVTGLGFGQNT
                     LAALITRGLHEITRLGIAYGGQPRTFAGLAALGDLVLTCTGGLSRNRRTGLELAAGKS
                     LDEITGETHMVAEGIRNSLAVSRLAAQKGVEMPITQQMVAVLYEGKDPRLAVEQLMTR
                     ELKAETEL"
     gene            2789..4342
                     /gene="guaA"
                     /locus_tag="VEL74_02170"
     CDS             2789..4342
                     /gene="guaA"
                     /locus_tag="VEL74_02170"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HYG61362.1"
                     /translation="MNMTDPGHQTVLILDFGSQFTQLIARRVRENRVYCEVHPFNLAL
                     EEIRRRRPVGVILSGGPQSVYEEGSPQGDPGLFELGIPILGLCYGMQWIAHALGGAVE
                     GAGRREYGRAEISVLEPGRLFDGLGTCETVWMSHGDHITEPPPGFRTLARTGSVPVVA
                     FENTERGIYGVQFHPEVSHTVSGGEMLRNFLYGACGARGDWRMASYLEEAVRTIRERV
                     GEGRVICALSGGVDSSVVAALLARAVGERSTAIFVDNGVLRKGEAVQVVHSLRDGLGI
                     PIRAVDARQRFLGELAGIEDPEKKRKAIGRVFIEVFRDEAKALQGVEFLAQGTLYPDV
                     IESTSVKGPSAVIKTHHNVGGLPAELGFELVEPLRFLFKDEVRQLGRELGLPEELIGR
                     HPFPGPGLAVRILGEVTAERVALLQEADAIFIQELRESGWYGKVSQAFAVLLPVKSVG
                     VMGDYRTYESVVAIRAVETHDFMTADWSPLPYELLGRVANRIVNEIQGINRVVYDVTS
                     KPPATIEWE"
     gene            4345..5289
                     /gene="arcC"
                     /locus_tag="VEL74_02175"
     CDS             4345..5289
                     /gene="arcC"
                     /locus_tag="VEL74_02175"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015479380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HYG61363.1"
                     /translation="MRILVALGGNALLRRGEEMTAENQRKNVRTAAEALAPLAEGNQL
                     VISHGNGPQVGLLALQGAAYEKVAPYPLDVLGAQTEGMIGYMIEQELGNILPFEQPFA
                     TILSMVEVDPEDPAFQNPTKPIGPLYPEEEARRLEAEKGWAMAPDGDKFRRVVASPKP
                     RRIFEIRPVKWLLEHGCIVICAGGGGIPTMYAPGRKLRGAEAVVDKDLASALLARELE
                     ADFFIMATDADAVYLDWGKPEQRAIRRASPQALAELDFASGSMGPKVEAAVEFAERTG
                     KTAAIGALSDLVRMTRGEAGTLVTQEAGELEVYVPGVG"
     gene            complement(5268..6056)
                     /locus_tag="VEL74_02180"
     CDS             complement(5268..6056)
                     /locus_tag="VEL74_02180"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HYG61364.1"
                     /translation="MLQSFVITLREGIEAFLIIAISLAYLKKTGRSSLIPAIHWGTGA
                     AVLLSIGAGVLLKRAANQALWEGILAVVAAVLVASLTVHMWKVARRMKRDIETRLERS
                     AERPGAGAFLGVFLFTLLMITREGMETALLMNALLFQVKSTQLILGAIGGILAAALLA
                     VLWSRYGHRINLARFFQVTAAFLLVFTVQLLLYGFHELTEANLFPNSEALHWATEPYG
                     PDGRYGQYLTYLMVLLPMGWLAVSMIFGKDRPVGTTPPQPTPGT"
BASE COUNT          929 a         2219 c         2402 g          936 t
ORIGIN      
        1 ccggtctatg ccgagagcga ggaggatacg ctcgagggcg tggtgggcat gctgctgaag
       61 gccgcggggg cgaccctcac ggtggccgag tcgtgcaccg gcggcctcct gggcgagcgc
      121 ctcacccgag tgccggggag cagcgactac ttcctgggcg gcgccatcgt ctacaccaac
      181 cgtctgaaga ccgagctggc cggggtgccg ccggagctcc tgcaggagca cggcgccgtc
      241 tccgagccgg tggcccgggc gatggccgag ggggtctgcc gtgccctcgg cagcgactac
      301 ggcatcgcgg tcaccggcgt cgcgggtccg ggcggcggca gcgaggccaa gccggtaggc
      361 accgtgcaca tcgcggtcgc cggccccgag gggacgtccc atcgccgcgt ccgctttccc
      421 ggcgaccggc agcgggtgcg ccagcactcc agccagctcg ccctggacat gctccgccgc
      481 aagctgctgg ggctcgatcc ccaggggatg gggtggcggt gaggctgttc gtcgccgtgg
      541 aggtgcccga ggcggtgcgg cgggaggtgg cgcgccgcgt ggcctcggtg cgcgaccggc
      601 tcccgcaggc gcgctgggtg gatcccgaga tgatccacct caccatggtc ttcctcggcg
      661 aggtgcccga ggaccgcgtc cccgcgctgg ccgaggcgct ccggcaggcc ttcgccccct
      721 atccgccgct cccgatgcgg ctctccggcg ccggcacctt cccgcctggc cggcccgccc
      781 gggtggcctg gctgggcctc gacgcgccgg acgacctgac ctctctccaa ggggacgcgg
      841 tccgggccgc ggtggcggcc gtggagcacg agccggagga ccggccgtac cgcgcccatg
      901 tgacgctggc gcggtgccag tcgccctggc gacgggacgc cgtcgacaag ttcatcgcgg
      961 ccttcccggg ggagaccggg cagccgtggg tggcggaccg gggcgtcctc atggagagca
     1021 agctgtcgcc caagggggcg cgctaccgcg tggtcgaagc cttccccttg gaggggccgt
     1081 ccttggtgac gggggacgag tgagcaccgg caccgcgctg gcgatcatcg ccgccgccta
     1141 cctggtgggc tcgatcccct ggagctatct gatcgtccgc ctgcggcagg ggatggacat
     1201 ccgggcggtg gggagcggca acgtaggggc gaccaacgtc ctgcgcaccg ccggcaaggg
     1261 ggcggggatc gtggccctgc tgctcgacgc cggcaagggg gtggcggtgg tcctcgtcac
     1321 ccgcgccctg gacgcgccgg cgtacgtcgt ctgcgtctcc gccgtcgcgg tggtgctcgg
     1381 ccacgtcttc ccggtgttcc tgcggttccg cggcggcaag ggggtggcga cggcggccgg
     1441 ggcgctgggc gcgctcgccc cggtggtgct cgccctgtcg ctgctcggat tcgtcgtcat
     1501 cgtgctctgg aaacgctacg tctcgctggg ctccatcctg gccgccgcgc tcttcccggt
     1561 cttcgcctgg atcggcagcc ggctcgggtg ggtgcggcac gaaggggcgt gggtggtgct
     1621 gtcgtccgcg gcgatcgcgg cggtggtgct cgcccgccac gcctccaacc tgcggcggct
     1681 cctcaaggga accgagcgcc ggctggggga gccccggagc atccggcaat gagcaggccg
     1741 tgagggaggg aagtcgatga agcagatcgc cgttctcggc gcgggctcct tcgggaccgc
     1801 cctcgccgtc catctcgcct ccatgggggc caacgcgggc ggcaaccgcg tccgcctgtg
     1861 ggcccgcaat ccggagctcg cccgccagat ggccgaggag cgggccaatc ccgagtacct
     1921 gccggacgtg gagatccccg ccggcgtcga gcccaccgcg gagatggagc cgctcgccga
     1981 gtgcgacatc gtcctggtgg tcgtcccctc gcacggcttc cgcggggtgc tgcgccagtt
     2041 ccttcgcgcg catccggcgg accggccggc ggtcatcgtc tcggcgacca aggggatcga
     2101 gaccgacacc ctggcgcgca tgagccaggt ctccttcgaa gagggcacgg ccgccggccg
     2161 cgaggtgcgc ttcgccaccc tctcggggcc gacgttcgcc gccgagctgg cgcgcaacag
     2221 cccgagcgcc gccgtcgtcg cctccgagga cgccgcgctc gccaccgaga tccgggagcg
     2281 cctgtcgacc aggctgctgc ggctctactc gtcgtccgac gtggtcggcg tggagctggg
     2341 cggcacggtc aagaacgtgg tcgcgatcgc cgccggcacc gtgaccggcc tgggcttcgg
     2401 ccagaacacc ctggccgcgc tgatcacccg cggcctgcac gagatcaccc gcctggggat
     2461 cgcctacggc ggccagccgc gcaccttcgc cggcctcgcc gccctggggg atctggtgct
     2521 cacctgcacc ggcggcctgt cgcgcaaccg gcgcaccggc ctggagctgg cggcgggcaa
     2581 gagcctggac gagatcaccg gcgagaccca catggtggcg gagggcatcc gcaactcgct
     2641 ggcggtgtcg cgcctcgccg cccagaaagg cgtcgagatg cccatcaccc agcagatggt
     2701 cgccgtcctc tatgaaggga aagacccacg cctggccgtc gagcagctca tgacgcgcga
     2761 gctcaaggcc gagacggagc tctgatcgat gaatatgacg gatcccggtc accagaccgt
     2821 tctgattctg gacttcggca gccagttcac ccagctcatc gcccgccgcg tgcgcgagaa
     2881 ccgggtctac tgcgaggtcc atcccttcaa cctcgctctg gaggagatcc ggcggcggcg
     2941 accggtcggc gtcatcctct cgggcggccc gcagagcgtc tacgaggagg gctcgccgca
     3001 aggggatccc ggcctcttcg agctggggat ccccatcctg ggcctctgct acggcatgca
     3061 gtggatcgcc cacgcgctcg gcggcgccgt cgagggggcg gggcggcgcg agtacggccg
     3121 cgccgagatc tcggtgctcg agcccggccg cctgttcgac gggctgggca cgtgcgagac
     3181 cgtgtggatg agccacggcg atcacatcac cgagccgccc cccggcttcc ggaccctggc
     3241 gcgcacgggg agcgtgccgg tggtggcgtt cgagaatacg gagcggggga tctacggcgt
     3301 ccagttccac cccgaggtct cgcacaccgt ctccggcggc gagatgctgc gcaacttcct
     3361 gtacggcgcc tgcggcgcgc ggggggactg gcgcatggcc tcctatctgg aggaggcggt
     3421 gcgcaccatc cgtgagcggg tgggggaggg gcgggtgatc tgcgcgctct ccggcggcgt
     3481 cgactcgtcg gtggtggcgg cgctcctggc gcgggcggtg ggggagcggt cgacggcgat
     3541 cttcgtggac aacggcgtgc tgcggaaggg ggaggccgtc caggtggtcc acagcctgcg
     3601 cgacgggctg ggcatcccca tccgggcggt ggacgcgcgg cagcgcttcc tcggcgagct
     3661 cgccggcatc gaggacccgg agaagaagag gaaggccatc ggccgggtgt tcatcgaggt
     3721 cttccgggac gaggcgaagg ccctccaggg ggtcgagttc ctggcccagg ggacgctcta
     3781 tcccgatgtc atcgagtcga cctcggtgaa ggggccctcg gcggtgatca agacccacca
     3841 caacgtcggc ggcctgccgg cggagctggg cttcgagctg gtggagccgt tgcgcttcct
     3901 gttcaaggac gaggtgcgcc agctcggccg cgagctcggc ctgcccgagg agctgatcgg
     3961 ccgccatccg ttccccgggc cgggcctcgc ggtgcgcatc ctgggcgagg tgacggccga
     4021 gcgggtcgcc ctgctccagg aggcggacgc catcttcatt caggagctgc gggagagcgg
     4081 ctggtacggc aaggtgagcc aggcgttcgc ggtgctcctg ccggtcaaga gcgtcggcgt
     4141 gatgggcgac taccggacct acgagagcgt ggtcgccatc cgcgccgtcg agacccacga
     4201 cttcatgacc gccgactgga gcccgctgcc gtacgagctg ctcggccggg tcgccaaccg
     4261 catcgtcaac gagatccagg ggatcaaccg ggtcgtctac gacgtcacca gcaagccccc
     4321 ggcgaccatc gagtgggagt aggaatgcgc atcctggtag ctctgggcgg caacgccttg
     4381 ctgcggcgcg gcgaggagat gaccgccgag aatcaacgga agaacgtgcg gaccgcggcc
     4441 gaggcgctgg cgcccctcgc cgaggggaac caactcgtca tctcgcatgg caacgggccg
     4501 caggtggggc tcctggcctt gcagggcgcc gcgtacgaga aggtggcacc ctacccgctc
     4561 gacgtcctgg gcgcccagac cgaggggatg atcgggtaca tgatcgagca ggagctgggc
     4621 aacatcctgc cgttcgagca gccgttcgcg accatcctct ccatggtcga ggtcgatccc
     4681 gaggacccgg cgttccagaa tccgaccaag ccgatcggtc ccctctaccc ggaggaggag
     4741 gcgcggcgcc tggaggcgga gaaggggtgg gccatggccc ccgacggcga caagttccgg
     4801 cgggtggtgg cctcgcccaa gcccaggcgg atcttcgaga tccggcccgt gaagtggctg
     4861 ctggagcacg gctgcatcgt catctgcgcc ggcggcggcg gcatccccac catgtacgcc
     4921 ccgggacgca agctgcgcgg cgccgaggcg gtcgtggaca aggatctggc gagcgccctc
     4981 ctcgcccgcg agctggaggc cgacttcttc atcatggcga cggacgccga cgcggtctat
     5041 ctggattggg gcaagccgga gcagcgggcc atccggcgcg cctccccgca ggccctcgcc
     5101 gagctcgact tcgcctccgg ctccatgggc cccaaggtcg aggcggccgt cgagttcgcc
     5161 gagaggaccg gcaagaccgc cgccatcggc gccctctccg acctcgtccg catgacgcgc
     5221 ggcgaggccg gcaccctggt cacccaggag gccggcgagc tggaggtcta cgtgcccggg
     5281 gtcggctgag ggggcgtggt gccgaccggg cggtccttgc cgaagatcat cgagacggcg
     5341 agccagccca tggggaggag caccatcagg taggtgaggt actggccgta gcggccgtcc
     5401 ggcccgtacg gctccgtcgc ccaatgcagc gcctcgctgt tggggaagag gttggcctcg
     5461 gtcagctcgt ggaaaccgta gagcaggagc tggacggtga agaccagcag gaaggcggcc
     5521 gtcacctgga agaagcgcgc caggttgatg cggtggccgt agcgcgacca gagcacggcg
     5581 agcagggcgg cggcgaggat cccgccgatg gcgccgagga tgagctgggt cgacttcacc
     5641 tggaagagca gggcgttcat cagcagcgcc gtctccatgc cctcccgggt gatcatcagc
     5701 aaggtgaaga ggaagacgcc gaggaaggcg ccggcacccg gccgctcggc cgagcgctcg
     5761 agccgggtct cgatgtcccg cttcatccgc cgcgccacct tccacatgtg caccgtgagc
     5821 gaggccacga ggaccgcggc gacgaccgcc aggatcccct cccagagcgc ctggttcgcc
     5881 gctctcttga gcagcacgcc cgctccgatg ctgagcagca ccgccgcgcc cgtcccccag
     5941 tggatggccg gaatcagact ggagcggccc gtcttcttca gataggcgag gctgatggcg
     6001 atgatcagga acgcctcgat cccctcgcgc aacgtgatga caaaggactg cagcatggtc
     6061 gatgactccg tgcctgttgg ttctgttgag attcagtctc aagttcgaga tgtaagcgta
     6121 gcctacctgt aattagcccg tcaaccgggc ggggagggcg tcgaaccggt gcgggaggcg
     6181 cttcggcggc agtcgagcga gggctttgag gagggaacgc cgaagatccc cgggagcgca
     6241 tccccatccc tgtcgagacg gaggtcatcc ttcccggggc ggccgttgcc gcacgaggcg
     6301 aagtgaccat ctccccggta ggaccggcgt acccggaatt cggccgccgt accccggcaa
     6361 acgttcgtcc tacccgaggt acggtcgtca tcccgaggga aatggcgctg atctcgagag
     6421 ggatggtctc cgtaccccgg gtagggaggc catccccgcg gggatgaggg ccgtaccccg
     6481 ggtagg
//
LOCUS       DASPJI010000082         4655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_806434, whole genome shotgun sequence.
ACCESSION   DASPJI010000082 DASPJI010000000
VERSION     DASPJI010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806434"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VEL74_02185"
     CDS             <1..661
                     /locus_tag="VEL74_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61365.1"
                     /translation="GAAPLRVESELITAAGGVIDVFQEVAPGQTVAVVNQPADRFQTV
                     LVTFQDPLERWDSVHVELERAPGEARRVLALSAVEPSGRWSAERPAGAPRTFRYHTRQ
                     VGRDGALRDDGWKEAAGSLLLVGDPDVQVGSVQVILLGPPDLMAVQLRLVSLAPPPGV
                     EGAVETLIEPGQPPFQARLPFRRGAERRYRVEGQVFLADGERQIDPRDETAEVLLLQV
                     G"
     gene            complement(733..1440)
                     /locus_tag="VEL74_02190"
     CDS             complement(733..1440)
                     /locus_tag="VEL74_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61366.1"
                     /translation="MLDPQARFTLPGLPGVLIFPDDHLAGGFYALPEVPRVALDGGGA
                     PQISLMLYGRKGAGGAFEATGGILSLTTTLELTPEEDEAIRSEMARRLAAQDPPVPGR
                     PQKLSPEWIDTEVTVHLIPGFDLKGKPSMTGGNLCSFQHKLTADQAKELQKAWNDGLK
                     DASATYRATVRAAPRAGSSFEIRSAACAMQDEKGSNSSASLHVQSAVTQAAPLPLTLE
                     GPLRAEGLQERLSTVGL"
     gene            complement(1461..4418)
                     /locus_tag="VEL74_02195"
     CDS             complement(1461..4418)
                     /locus_tag="VEL74_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61367.1"
                     /translation="MPEPTASTPPAVASPISDLLFPITFALDGEIRFISEMRTARFAF
                     FDVPVFTQEEADKLTLPSAEIQTLVDKNGGRWRPLSTWLQDPAQAVVRCRRSGLTVKL
                     EAADRAISLLPFAPGEGSEHGQPSLSATLHLGDGLPDEPVPVVRLDRELGQLQLTIPF
                     EDSDAGRDRYARTVEALSAAASEARIELDHSHVLSMKSEAPLTFETAILILDPGEAEM
                     TAKSHWESLGGVLTSGADACSWAPGRLDVFVRGTDETLWHRGYEGGWFDWGSLGGRIT
                     SDPGAVSWAPGRIDVFARGGDNALWHIGYEGGWSHWESLGGGLSSGPDVCSWAPGRLD
                     VFVRGMDDALWHKWYDNGWSEWESLGGGLSSDPSAVSWSSGRIDVFVRGMDNALWHKG
                     FDNGWSGWESLGGGLASGPDVCSWAPGRLDVFALGLDNALWHRWYDNGWSEWERLGGH
                     LTSDPGTVSWGPGRIDVFARGTDNALWHRWYDGSWHAVPAEGVVQQVTADNVLGVPLG
                     RRRDTQPEAFPDLPAPGEGRWKEVPARTAGARPLFYRSSDQGDVFFHLPASYRLGLRA
                     TGDGGDGGPLPPMRPVLYRAPAGAEERDRVQVTLTAVPTIDAADREHLRAHIQTRVLG
                     GLVPFVRLAPASGLTAAFTAPFTAAGGVLPPGITYRGEAVAVDREINFQFDMPARLYP
                     LFCELLRQGLRGRVDLTGDELQRSVDVSLDFRSVVADGLGVRALPMADGAVPLTVENL
                     LDRPVRLGAVRAILMDQSREGNADLVFQAEGRDLLQQARRLEPRGTDGATLELRVPLV
                     NGATWDRIAVAVGTAEVDGGSPADWLDRVHRDSSLQPMDFAIEVSASIPAAARERIEG
                     VQVQLLRPGSAEVRAERMLRPGDPPWNLRVSLSLADLAASTGGELPRFVLESFTAYHD
                     LGPGLPQRTLVDLNAGTAVVRALAETAASRYTLVRGTERETGRTRAQVEEALPRMRAA
                     GEVWQLFVEAPQT"
BASE COUNT          668 a         1642 c         1685 g          660 t
ORIGIN      
        1 cggtgccgct cctctccgtg tggagtcgga gctgatcacg gcggccggtg gagtcatcga
       61 cgtcttccag gaggtggcgc ccggtcagac ggtggcggtc gtcaaccagc ccgccgaccg
      121 cttccagacg gtgctggtga ccttccagga cccgctggag cgctgggatt ccgtgcacgt
      181 agagctggag cgggctcctg gcgaggcgcg gcgagtgctg gcgctctccg ccgtcgagcc
      241 ctccggccgc tggtcggcgg agcgcccggc cggagcgccg cgcaccttcc ggtatcacac
      301 ccgccaggtg ggccgcgacg gcgcgcttcg tgatgacggc tggaaggagg cggccggctc
      361 gctgctgctg gtgggcgatc cggacgttca ggtggggagc gtgcaggtga tccttctcgg
      421 gccgccggac ctcatggccg tccagctccg gctcgtctcc ctcgcccctc cgccgggtgt
      481 cgaaggcgcc gtcgaaacgc tgatcgagcc cggccagccc cccttccagg cccgcctccc
      541 cttccgccgg ggcgccgagc ggcgctatcg agtcgagggg caggtcttcc tggccgacgg
      601 cgagcggcag atcgatcccc gcgacgagac cgcggaggtt ctgctgctgc aagtgggtta
      661 ggggcgaggg gggcacggcc ggttcttctc cgctccatac aggcgagatc tctcggcttt
      721 cgttaccaga cctcagagcc cgaccgtgct cagccgctcc tggaggcctt cggcgcgcag
      781 agggccctcc agagtgagcg gcagcggcgc ggcctgggtg acggcggact ggacgtgcag
      841 cgaggcggag ctgttggaac ctttttcgtc ctgcatggcg caggcggccg agcgtatctc
      901 gaagctcgat ccggcgcggg gcgcggcgcg gaccgtggcg cgataggtgg ccgacgcatc
      961 cttcagtccg tcgttccatg ccttctggag ctccttcgcc tgatcggcgg tcagcttgtg
     1021 ctggaacgag cagagattgc cgccggtcat cgacggcttg cccttcagat cgaagccggg
     1081 gatcagatgg accgtgacct cggtgtcgat ccactccggg ctcagcttct gcggcctgcc
     1141 gggcaccggc gggtcctgcg ccgccagacg gcgggccatt tcgctccgga tcgcctcgtc
     1201 ctcctccggc gtgagctcca gcgtggtggt gagtgaaagg atcccgccgg tggcctcgaa
     1261 cgcgccgccc gcccccttgc gtccatagag catcaggctg atctgtggcg cgccgccgcc
     1321 atccagggcg acgcgcggga cctcgggcaa ggcatagaag cccccggcga gatggtcgtc
     1381 gggaaaaatc aggacgcccg gcaatccggg cagggtgaag cgggcttggg ggtccagcat
     1441 ggcggcctcc tgaaaaaacc ttacgtttgt ggcgcctcga cgaagagctg ccagacctcg
     1501 cccgcggcgc gcatccgggg cagcgcctcc tccacctggg cacgggtccg gccggtctcg
     1561 cgctcggtgc cgcgcaccag ggtgtagcgg ctcgccgcgg tctcggcgag ggcgcgcacc
     1621 acggccgtgc cggcgttcag gtccaccagc gtgcgctgcg gcagacccgg ccccaggtcg
     1681 tgataagcgg tgaaggactc gaggacgaag cggggcagct cgccgccggt ggaagccgca
     1741 aggtcggcca ggctcaggct gacgcgcaga ttccaggggg gatcgccggg ccgcagcatc
     1801 cgctcggccc gcacctcggc gctcccgggg cgcaggagct ggacctgcac gccctcgatc
     1861 cgctcacggg ccgcggccgg aatgctcgcc gagacctcga tggcgaagtc catgggctgg
     1921 agcgacgagt cgcggtggac acggtcgagc cagtccgcgg gtgagccgcc gtccacctcg
     1981 gcggtgccga ccgccacggc gatccggtcc caggtggccc cgttgacgag ggggacgcgc
     2041 agctccagcg tggcgccgtc cgtgccgcgg ggctccaggc gcctcgcctg ctgaagcagg
     2101 tcgcgcccct cggcctggaa caccaggtcg gcgttccctt cacggctctg gtccatgagg
     2161 atcgcgcgga ccgcccccag ccggaccggg cggtccaaca ggttctccac cgtcagcggc
     2221 acggcgccat cggccatggg gagggcgcga acgccgaggc cgtcggccac cacgctacgg
     2281 aaatcgaggg agacgtccac cgagcgctgc aactcgtcgc cggtcaggtc cacgcggccg
     2341 cggaggccct gacgcagcag ctcacagaaa aggggataga ggcgggccgg catgtcgaac
     2401 tggaagttga tctcccggtc gacggccacg gcctcgccgc ggtaggtgat ccccggtggc
     2461 agcacgccgc ccgccgcggt gaacggcgcc gtgaaggccg cggtcagtcc cgacgccggc
     2521 gccaggcgga cgaacggcac gaggccgccg aggacccgcg tctggatgtg ggcccgcagg
     2581 tgctcccggt ccgccgcgtc gatcgtgggg acggcggtga gcgtcacctg gacacggtcg
     2641 cgctcctccg cgccagccgg ggcgcggtag aggaccggcc gcatgggcgg cagcgggccg
     2701 ccgtcgccgc cgtcgccggt ggcgcggagg ccgagccggt aggaggcggg gagatgaaag
     2761 aagacatccc cctggtcgga gctccggtag aacaggggcc gcgctccggc cgtccgcgcc
     2821 ggcacctcct tccaccgccc ctcccccggc gccggcaggt ccggaaaggc ctcgggctgg
     2881 gtgtcgcggc gccggcccag cggaaccccg agcacattgt cggcggtgac ctgctggacg
     2941 acgccctccg cgggtacggc gtgccaggag ccgtcgtacc accggtgcca gagcgcgttg
     3001 tccgtgccgc gggcgaagac gtcgatccgt ccggggcccc aggagacggt gccgggatcg
     3061 gaggtgagat ggccgccgag ccgctcccac tcggaccagc cgttgtcata ccagcggtgc
     3121 cagagcgcgt tgtccagtcc gagggcgaag acgtccagcc gccccggagc ccacgagcag
     3181 acgtcggggc ccgaggccag cccgccgccg agcgactccc agccggacca gccgttgtcg
     3241 aaccccttgt gccagagcgc gttgtccata ccgcggacga agacgtcgat gcgcccggac
     3301 gaccaggaga cggcgctggg gtcggagctc aggccgccgc cgagcgattc ccactcgctc
     3361 cagccgttgt cgtaccactt gtgccagagc gcgtcgtcca tgccgcggac gaagacgtcc
     3421 agccggccgg gcgcccacga gcagacgtcc ggtcccgagc tcagcccgcc gcccagcgac
     3481 tcccagtggc tccagcctcc ctcgtacccg atgtgccaga gggcgttgtc cccgccgcgg
     3541 gcgaagacat cgatgcgccc cggggcccag gagacggccc ctgggtcgga ggtgatccgc
     3601 ccgccgagcg atccccagtc gaaccatccg ccctcgtacc cgcgatgcca gagggtttcg
     3661 tccgtaccgc ggacgaagac gtccagccgg cccggagccc aggagcaggc atcggcgccg
     3721 ctggtgagga cgccgccgag cgactcccag tgcgacttgg cagtcatctc cgcctcaccg
     3781 gggtccagga tgaggatggc ggtttcgaac gtgagcggcg cttcgctctt catcgacagc
     3841 acatgcgagt ggtccagctc gatccgtgcc tcggacgcgg cggcgctcag ggcctcgacc
     3901 gtccgggcgt agcggtcacg gccagcgtcg ctgtcctcga aaggaatggt gagctggagc
     3961 tgccccagct cgcggtcgag ccgcaccacg gggaccggct cgtcgggaag gccgtcgccg
     4021 agatgcaacg tggcgctgag gctgggctgc ccatgctcgc tgccttcccc gggggcgaag
     4081 gggaggagcg agatggcgcg gtcggcggct tcgagcttga ccgtgaggcc gctcctgcgg
     4141 caccgaacca ccgcctgggc cggatcctgg agccaggtcg agagcgggcg ccagcgtccg
     4201 ccgttcttgt cgacgagcgt ctggatctcg gccgacggga gggtcagctt gtccgcctct
     4261 tcctgggtga acaccggtac gtcgaagaac gcgaaccgcg ccgtccgcat ctcgctgatg
     4321 aaccggatct cgccgtccag tgcgaaggtg atcgggaaca gcaggtcgga aatcggcgag
     4381 gcgacggccg ggggtgtgct cgcggtgggc tcgggcatgg tccgctcctg gaaggcagga
     4441 gccggcgaac ggcgccggct ccgcctctct caggcgcgga aagatgggcg agcgttactg
     4501 ggcaggtcta cctcgaacgc tctgacccgt ctcgaccctc ttcagacctg cctcgacccc
     4561 agggtcaagc cgttcgaccc tgttcaaacc tgcctcgacc ctcgggtcaa gccgttcgac
     4621 cctctttaaa cctgcctcga ccctggggtc aagcc
//
LOCUS       DASPJI010000083         2581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_407894, whole genome shotgun sequence.
ACCESSION   DASPJI010000083 DASPJI010000000
VERSION     DASPJI010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407894"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2535)
                     /locus_tag="VEL74_02200"
     CDS             complement(<1..2535)
                     /locus_tag="VEL74_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG61368.1"
                     /translation="MDSLRRAFFVGDALTRRDVERLRALAPRVACVNLYGSTETQRAV
                     AYHEVRHEVRDGEAPEVYPLGRGMPDVQLLVFNRSGDLAGIGEAGEVGVRSPHLAGGY
                     LGDPGLTAERFVTNPFTGRAGDRIYRTGDLGRYRPDGEVEPLGRVDHQVKIRGFRVEL
                     GEIEAVLGRHPAIREAVVVARGESGDRRLIAYAVPAAAAPDARELRGCLRARLPEYMV
                     PAAFVFLDALPLTPNRKVDRKALPEPGRDEPGHAGTEEAAAPRTPVEELLAAIWSEVL
                     ETGRIGPEDDFFALGGHSLRVTQVLARVREAYGVELPVRSVFESPTLAGLAGRIEEVL
                     RAGSARQALPLVPVPRHKDLPLSFAQERLWFLDRLAPGSAAYNMPAALRLRGVLDPAV
                     LERTLHEIVRRHEVLRTTFPEADGEPRQRVHPFRPFPLPVLDLRALPEEVRDAEAARQ
                     ARAEAVRPFDLARGPLLHAALLRMEERDWMALFTTHHIVSDGWSVGVLVREIAALSAG
                     ASLPGLPVQYADFAVWQRGWLQGEALAAQVAFWREALAGAPAVLDLPLDRPRVPGEGR
                     AGLRTAALPPELAGALRDLGRRHGATLFMILLAAFQSLLHRLTGEDDLVVGTPIANRT
                     HPGLEGLIGFFVNTLALRSRLEGADPGFIELLGRTRAAALAAYAHQDLPFERLVEELR
                     VERSLRHAPVFQVMLVLQNAPKAELELPGLTLAPVPPALGAAQFDLSLSFMEAGSEVR
                     AALEYDARLFDATTAERLLDRLGVLLRTIAVHPERRLSDLPVMAEAERHQVTIEWTEP
                     DPSAPVTFPLHRRFEEQAAQDPGALAIGGDGRHLTYRELDRGANRLA"
BASE COUNT          357 a          930 c          982 g          312 t
ORIGIN      
        1 cgcgagacgg ttcgcccccc ggtccagctc gcggtaggtg aggtgcctgc cgtcgccgcc
       61 gatcgccagc gcgcccgggt cctgagcggc ctgctcctcg aagcgccggt gcaatgggaa
      121 ggtcaccgga gccgacgggt ccggctccgt ccattcaatt gttacctggt gccgctccgc
      181 ctcggccatc accggcaggt cggacagccg gcgctccgga tggacggcga tcgtgcgcag
      241 gaggacgccg agccggtcca gcaggcgctc cgcggtggtc gcgtcgaaga ggcgggcgtc
      301 gtactccagc gccgcgcgga cttcactccc ggcttccatg aaggagaggc tgaggtcgaa
      361 ctgggcggcg ccgagggcgg gcgggacggg cgcgagggtg agcccgggca gctccagctc
      421 cgccttgggc gcgttctgga ggacgagcat cacctggaag accggcgcgt ggcggaggct
      481 gcgctcgacc cgtagctcct ccaccagcct ttcgaacggc aggtcctggt gggcgtaggc
      541 cgcgagcgcc gcggcgcggg tgcggccgag cagctcgatg aagcccgggt cggccccctc
      601 gagccggctg cgcagggcca gggtgttgac gaagaagccg atcagccctt cgagcccggg
      661 gtgggtgcgg ttggcgatcg gcgtgccgac caccaggtcg tcctcgccgg tgagccggtg
      721 gagcagggac tggaacgccg ccaggaggat catgaacagg gtggcgccgt ggcgcctccc
      781 caggtcccgc agggcgccgg ccagctccgg cggcagcgcc gccgtgcgca ggccggctct
      841 gccctccccc ggcacacggg gccggtcgag cggcaggtcg aggaccgccg gagcgccggc
      901 cagcgcctcg cgccagaagg cgacctgggc cgccagcgcc tcaccctgga gccagccgcg
      961 ctgccagacc gcgaagtcgg cgtactggac cggcagacca gggagggatg ctcccgcgga
     1021 cagggccgcg atctcgcgca ccaggacgcc caccgaccag ccgtcgctca cgatatgatg
     1081 ggtggtgaac agcgccatcc agtcgcgctc ctccatccgg agcagcgcgg cgtgcagcag
     1141 cggtccgcgg gcgaggtcga agggccgcac cgcctcggcc cgcgcctgcc gggccgcctc
     1201 ggcgtcgcgg acctcctcgg ggagagcgcg gaggtcgagg accggaaggg ggaacggccg
     1261 gaacgggtgg acccgctggc gcggctcgcc gtccgcctcc gggaaggtgg tgcgcaggac
     1321 ctcgtgccgg cggacgatct cgtgcagcgt ccgctccagg acggcggggt cgagcacgcc
     1381 ccggagccgc agggcggcgg gcatgttgta ggccgcgctc cccggcgcga ggcggtcgag
     1441 gaaccagagg cgttcctggg cgaacgatag cggcaggtcc ttgtgtctag gaaccggcac
     1501 cagcggcagc gcctggcgcg cggacccggc acgcagaacc tcttcgatgc gccccgccag
     1561 ccccgccagt gtcgggctct cgaaaacgct gcgcaccggc agctcgacgc cgtacgcctc
     1621 gcggacgcgg gccaggacct gggtaacccg gagcgagtga ccgccgagcg cgaagaagtc
     1681 gtcctccggg ccgatccggc cggtctccag gacctcgctc catatcgccg cgagcagctc
     1741 ttcgaccggc gtccgcgggg ccgcagcctc ttcggtcccg gcgtggccgg gctcgtcccg
     1801 tccgggctcg ggcagagcct tgcggtccac cttgcggttc ggggtgagcg gcaacgcgtc
     1861 caggaagacg aaagcggccg gaaccatgta ctcggggagg cgggcgcgca ggcagccccg
     1921 cagctcgcgg gcgtccggcg ccgccgcggc aggcacggca taggctatga gacggcggtc
     1981 gccgctctcg ccgcgcgcca cgaccacggc ctcacggatc gcgggatggc ggccgaggac
     2041 ggcctcgatc tccccgagct cgacgcggaa gccgcgaatc ttgacctgat ggtcgacgcg
     2101 gcccagcggc tcgacctcgc cgtccggccg gtagcggccg aggtcgcctg tacggtagat
     2161 ccggtcgccg gcgcggccgg tgaacgggtt ggtcacgaac cgctcggcgg tcaggccggg
     2221 atcgccgaga tagccgccgg cgagatgggg gctgcgcacg ccgacctcgc cggcttcgcc
     2281 gatgcccgcg aggtcgccgg accggttgaa gacgagaagc tgcacgtccg gcatgccacg
     2341 gccgagcgga tagacctcag gcgcctcgcc gtccctcacc tcgtgtctca cctcgtggta
     2401 ggcgacggcg cgctgggtct cggtcgagcc gtagaggttg acgcaggcga cccgcggcgc
     2461 cagggcgcgc agccgctcga cgtcccggcg ggtcagcgcg tcgccgacga agaaggcgcg
     2521 gcgcagggaa tccaccgtgg gcccctcccc ccggccccct ccccgctcgt tcctcgcgga
     2581 g
//
LOCUS       DASPJI010000084         6554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_806619, whole genome shotgun sequence.
ACCESSION   DASPJI010000084 DASPJI010000000
VERSION     DASPJI010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806619"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VEL74_02205"
     CDS             <1..1209
                     /locus_tag="VEL74_02205"
                     /inference="COORDINATES: protein motif:HMM:NF040048.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HYG61369.1"
                     /translation="GAYLAGSHHWDVLVDNAERPGGFDFTAYLDRLESWGHNLIRLWT
                     HEAWTHEVRPLPYPRTGPGLARDGRLRYDLSRFDPEYFGRLRDRAEAAGRRGFRVIVM
                     LWNGWSLHDKGEGNPWHHHPMNRANNVNGIDGDPDACGDGADVHTLRVPAITRLQEAY
                     LEKVVDTVGDLDHVLWEISNESPGDSVAWQYHMIRYLRAYEAARGRSHPIGMTATFPG
                     GTNRDLLSSPADWISPSRKGGWMTSPPPADPTGKVVLLDTDHLWGIGGDRRWVWKSFL
                     RGHHILYMDPLDADPVREGARRAMGHARRFAERMDFAALVPEPRLASTGYCLAKRVPG
                     EEEVLVYQPRSGWARSFSVDLTGIAGELTAEWFDPEAGATTRAERVTAGARRRLRAPF
                     PGDSVLYLRR"
     gene            complement(1163..2320)
                     /locus_tag="VEL74_02210"
     CDS             complement(1163..2320)
                     /locus_tag="VEL74_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61370.1"
                     /translation="MKLPSLRALVPRLILLGVSAVLSLLVAEVAVRMVRPQAVMVVSQ
                     GLYVPDPPRRYRLQPGFQGTVTNRAEFDTRVAINRAGLRGPEVGPRQPGTLRVLALGD
                     SFTFGVGAQQEETYPARLEMVLRERGVRAEVLNAGAPGFGVPDAVAWYEAHGVPLQPD
                     VAVLAVFMANDLQDASPGAFKVVAEDGYLVVPGERGGLKRWLYYHSHLYVLLKNAFSG
                     PLRKALGLGEAHAERELRAEFELYAEKPSEMIKGGAAATDRAVAEWVRAASARGDRVM
                     AVLVPSLLQVDDEAWRQSLARFGLDSSRLDQDRPTLLFRDIFQRHGVPVLDLTRPFRQ
                     AMGVGQRVYYPIDQHLTPEGYELMARLVADFLVKNRLTGAGTAPSPPGTAP"
     gene            complement(2335..2556)
                     /locus_tag="VEL74_02215"
     CDS             complement(2335..2556)
                     /locus_tag="VEL74_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HYG61371.1"
                     /translation="MKCHVCGSDMVPVATDLPFKLNDTTIVIVKSLPVLQCRNCSEYA
                     LEDAVMQRVDEILQRVDSQAELEIVRFAA"
     gene            complement(2974..4053)
                     /locus_tag="VEL74_02220"
     CDS             complement(2974..4053)
                     /locus_tag="VEL74_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HYG61372.1"
                     /translation="MEERHPSTPPPSVRGRGAADNPTNRFETISVELEEPGPETVPTV
                     FLRDASRSVLSRNDSPDVGFSVSLNPYRGCEHGCIYCYARPTHEYLGFSPGLDFESRI
                     LVKEDAPELLRKELMSPRYKPEAIAMSGVTDCYQPAERRLRITRRCLEVLAEFRNPVA
                     IITKNALVRRDTDVLAELAAHDAAMVGFSITSLDPEITRRMEPRTSHPRERLEALAEV
                     SSRGVPCAVLVSPVVPGLTDHEMPGILAAAKEAGATVAHFIPLRLPGPVAGLFEEWLE
                     RQFPDRKEKVLNRVRGMRGGRLNDPRFGNRMRGEGIFADQLRALFHTTCRKVGLDTGG
                     PELSTAAFRRPAAPRADGQLSLFDG"
     gene            4149..5537
                     /locus_tag="VEL74_02225"
     CDS             4149..5537
                     /locus_tag="VEL74_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61373.1"
                     /translation="MALSEEVRKTHRDLSAIDLEFRELAHEDPRFLDRSHFQPLTREN
                     ELLAYRLQPWLTFLGRDKLQELQRVSLGMYRLLQTLPMRIFEGDLNRLQEFYGFPSPF
                     ITEIVMSPPNGIDTIISRGDLIDTADGFKCIEFNFSPHLGGWESPILAGLHLSVPATA
                     EFLSSRGIPVAYTDTVQKLFRLVIRDVMEKGISRDGEINVGFLIPTTDPVESTGPKTR
                     YFDGELQRALAEVGGGLSGRVVAGNYASLSTRDNLLFSGGLRLHAAIDLTIGGNTQPA
                     VFRSFKSSNLSLFNGPVGPVLGTKRNLALLSEHAGTSAFSDEEREFIRRYIPWTRLVV
                     PGSVEFEGRSAPLDELLVARRERMVLKEGGSAGGKGVFLGKFTPEPEWREAIATAMQS
                     RGQWVVQEHFESLPYLYQSGDHGASVHDVIWGPFVFGGEYAGVILRMQPRAAGGAVNL
                     SLAATEGVVFEV"
     gene            5669..6004
                     /locus_tag="VEL74_02230"
     CDS             5669..6004
                     /locus_tag="VEL74_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HYG61374.1"
                     /translation="MKREKKADLLQGTLDMLILKAVSLGPLHGYGIIQRIHQMSGEML
                     QVEQGSLYPALYRIEQKGWVTSQWEVNETGRRAKFYSITRAGASQLEAEEASWDRLVL
                     AIAKVRQAT"
     gene            6017..>6554
                     /locus_tag="VEL74_02235"
     CDS             6017..>6554
                     /locus_tag="VEL74_02235"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HYG61375.1"
                     /translation="MRWLNVFSARVRALSDREAVLHDIDEEMRLHLEMATEANVQRGM
                     APAEARRAALSSFGHLDNLRDTAYGVRGGGIAETLWQDIRYGVRVLAKQKGYTAVAVL
                     TLALGIGANTSIFSVVHELLVRPLPYADPERIVTVWELTPEGREHNVASAANFLSWSG
                     QSTSFESMAAFSDQRVNLT"
BASE COUNT         1089 a         2281 c         2165 g         1019 t
ORIGIN      
        1 ggagcctacc tcgccggctc ccaccactgg gacgtcctgg tggacaacgc cgagcggccg
       61 ggaggtttcg acttcacggc ttatctcgac cggctggagt cctggggaca caacctcatc
      121 cggctgtgga cccacgaggc ctggacgcac gaggtccggc ccctccccta tccgcgcacc
      181 ggtcccgggc tcgcccgcga cgggcgcctc cgctacgacc tctctcgttt cgatcccgag
      241 tacttcggcc gtctccgcga ccgggccgag gctgcgggcc gccgcggctt ccgggtcatc
      301 gtgatgctgt ggaacggctg gtcgctccat gacaaggggg agggcaaccc ctggcatcac
      361 catccgatga accgggcgaa caacgtcaac ggcatcgacg gcgatcccga cgcatgcggt
      421 gacggggccg acgtccacac cctgcgcgtg ccggccatca cccggctcca ggaggcctat
      481 ctcgagaagg tggtggacac ggtcggggac ctggaccacg tcctctggga gatctccaac
      541 gagagccccg gcgattcggt cgcctggcaa taccacatga tccggtacct ccgggcctac
      601 gaggccgccc ggggcaggag ccatcccatc ggcatgaccg ccaccttccc gggcggcacc
      661 aaccgggacc tcctctccag ccccgccgac tggatctctc ccagccgcaa agggggctgg
      721 atgacgtcgc cgccgccggc tgaccccacc ggcaaggtgg tgctcctgga caccgatcac
      781 ctctggggga tcggcggcga ccgccgctgg gtctggaaga gcttcctgcg cggacaccac
      841 atcctctaca tggacccgct ggacgcggat ccggtgcggg agggggcgcg ccgcgccatg
      901 gggcacgccc ggcgcttcgc cgagcgcatg gacttcgcag ccctggtgcc ggagccccgc
      961 ctcgcctcca ccggctattg tctggcgaag cgcgtcccgg gagaggagga ggtcctggtc
     1021 taccagccgc gaagcggatg ggcgcgctcg ttctctgtag acctcaccgg catcgcgggg
     1081 gagctgaccg cggagtggtt cgaccccgag gcgggagcga cgacgcgggc ggagcgggtg
     1141 acggctgggg cgaggcggcg gctcagggcg ccgttcccgg gggactcggt gctgtacctg
     1201 cgccggtgag gcggttcttc accaggaagt ccgccaccag ccgcgccatc agctcgtacc
     1261 cctcgggagt gaggtgctgg tcgatagggt aatagacccg ttgtcccacg cccatcgcct
     1321 gccggaatgg ccgggtgaga tcgaggacgg gcacgccgtg gcgctgaaag atgtcccgga
     1381 acagcagggt gggtctgtcc tgatcgaggc gggacgagtc gaggccgaag cgcgccaggc
     1441 tctgcctcca ggcctcatcg tctacctgga ggagggacgg cacgagcacc gccatcaccc
     1501 ggtccccccg cgcggaggcg gcgcgtaccc actcggcgac agcccggtcg gtggcagccg
     1561 cccccccttt gatcatctcc gaaggcttct cggcatacag ctcgaactcc gcccggagct
     1621 cgcgctcagc gtgggcctcc ccgagaccca gcgccttgcg gagcggtccc gagaaggcgt
     1681 tcttgagcag cacgtacaga tgggagtggt agtagagcca ccgtttcaga ccgccccgct
     1741 cccctggcac caccagatag ccgtcctccg ccaccacctt gaacgcgccg ggggaggcgt
     1801 cctggagatc gttggccatg aacacggcga ggacggcgac atcgggctgc agggggacgc
     1861 catgcgcctc gtaccaggcg acggcgtcgg gaacgccgaa cccgggagcg cccgcgttga
     1921 gcacctcggc ccgaacgccg cgctcccgca gcaccatctc cagccgcgcc ggataggtct
     1981 cctcctgctg ggcccccact ccgaacgtga acgagtcccc cagagcgagg acgcgcaacg
     2041 tgcccggctg tcgcggcccg acctccggcc cgcgaagccc ggcacggttg atggcgacgc
     2101 gggtatcgaa ctccgcccgg ttggtgacgg tgccctggaa cccgggctgg agccggtacc
     2161 ggcgcggcgg gtcgggaaca tagagcccct gcgacaccac catcacagct tgaggccgaa
     2221 ccatccgcac cgccacctcg gccaccagca ggctgagcac ggcgctcacg ccgagcagga
     2281 tgagacgggg gacgagggcg cgaagggaag ggagcttcat ggacgaggga ggactcaagc
     2341 ggcgaagcgg acgatctcga gctccgcctg ggagtcgacc ctctgcagga tctcatcgac
     2401 ccgctgcatc accgcgtctt cgagcgcgta ctcactacaa ttcctgcact ggaggacggg
     2461 caggctcttc acgatcacaa tcgtggtgtc gttcagcttg aagggcagat ccgtcgccac
     2521 agggaccatg tccgagccgc acacgtgaca cttcatggcg ttcctctcct ggtcttccaa
     2581 tccgtctccc attcatcagg gctcggccgg tatgctgtca cgatccggac gttgtctcct
     2641 ggacatccac tccgaacagg acatgcaagg gtacaccgtg agctcgagtg cagaccaggt
     2701 agctcgggaa gtacttgtcc tcaggatagc tctcgataac ctcgtaggcc gaggtggact
     2761 cgatgacctc cgcgcgagga atcgaccgcc ctttcaagcg catgttgacg tgatacgtcc
     2821 aaaggacctt ccgctggctg acacatcgcc gaatgaaacc tagaggatct tctggaatcc
     2881 tgcctgtccc ggccatcaat gatgtccgat ctcctttccg cacaccccat ccgacttcca
     2941 gtatctcgga ctccaactta ccgggaaact tcctcagccg tcaaacaggc tgagctgacc
     3001 atccgcccgg ggagcggccg gccgccggaa ggccgccgtg gacagctccg gccccccggt
     3061 gtccagcccg accttgcggc aggtggtgtg gaagagggcg cggagctggt cggcgaagat
     3121 cccttccccc cgcatccggt tgccgaagcg cggatcgttg agccggccgc cgcgcatgcc
     3181 gcggacgcgg ttcagcacct tctccttgcg gtccgggaac tggcgctcca gccactcctc
     3241 gaacaggccg gcgaccgggc cgggcaggcg cagggggatg aagtgcgcca ccgtcgcccc
     3301 ggcctccttc gccgccgcga ggatgccggg catctcgtga tccgtcaggc cgggcaccac
     3361 cggcgacacc aggacggcgc agggcacgcc ccgggaggag acctcggcca gcgcctccag
     3421 ccgctcccgc gggtgagacg tccgcggctc catccgccgg gtgatctccg ggtcgaggct
     3481 ggtgatggag aaccccacca tcgccgcgtc gtgggcggcg agctccgcca ggacgtcggt
     3541 gtcccggcgc accagggcgt tcttggtgat gatcgccacc gggttccgga actcggccaa
     3601 cacctccagg caccgccgcg tgatccggag ccgccgctcc gccggctgat agcagtcggt
     3661 gacgccgctc atggcgatgg cctccggctt gtaccggggg gacatcagct ccttgcgcag
     3721 gagctccgga gcgtcctcct tcaccaggat gcggctctcg aagtcgagcc cgggagagaa
     3781 ccccaggtac tcgtgggtgg gccgggcata acagtagatg cacccgtgct cgcacccgcg
     3841 ataggggttg agggagaccg agaaccccac gtccgggctg tcgttccgcg acagcacgct
     3901 acgcgaggcg tcgcgcagaa acactgtcgg gaccgtctcc ggccccggct cctccagctc
     3961 gaccgagatc gtctcgaacc ggttggtggg attgtccgcc gctccgcgcc cccggacgct
     4021 gggcgggggg gtggaagggt gccgctcctc catgcgccca gattacgcca gtttgaaccg
     4081 gttgtcgacc cctgacgacc ctctcgggac ggtgataggc tctccagcca tctcatggag
     4141 gggtccggat ggctctatct gaggaggtgc ggaagacgca ccgcgatctg tcggcaatcg
     4201 atctcgagtt ccgcgagctg gcccacgagg acccgagatt cctcgaccgc tcacacttcc
     4261 aacctctgac ccgggagaat gagctcctcg cctatcgcct gcagccctgg ctgaccttcc
     4321 tcggccgcga caagctccag gagctccagc gcgtcagcct cggcatgtac cggcttctgc
     4381 agaccctgcc gatgcggatc ttcgaagggg acttgaaccg gctccaggag ttctacggct
     4441 tcccctcccc attcatcacc gagatcgtca tgagcccccc gaacggtatc gacaccatca
     4501 tttcgcgggg cgacctgatc gatacggctg acggcttcaa gtgcatcgag ttcaacttca
     4561 gccctcacct gggcggctgg gagagcccca tcctggccgg cctccacctg tcggtccccg
     4621 ccacggccga gttcctctcc agccgcggga tcccggtcgc gtacaccgac acggtgcaga
     4681 agctcttccg gctggtcatc cgggatgtaa tggagaaggg gatcagccgg gacggcgaga
     4741 tcaacgtcgg tttcctcatc ccgacgacgg acccggtcga gtcgaccgga ccgaagaccc
     4801 gatacttcga cggggagctc caacgggccc tggccgaggt gggcggcggt ctgtccggca
     4861 gggtggtggc cggcaactac gcgagcctgt ccacccggga caacctcctg ttctcggggg
     4921 gtctgcggct tcacgcggcg atcgacctca ccatcggcgg caacacccag cccgcggtct
     4981 tccgcagctt caaatcctcc aacctctccc tgttcaacgg cccggtcgga ccggtcctcg
     5041 ggaccaagcg gaacctggcg ctgctctccg agcatgccgg caccagcgcc ttcagcgacg
     5101 aggagcggga gttcatccgc cgctacatcc cctggacccg cctcgtggtt ccgggctccg
     5161 tggagttcga ggggagatcg gcccccttgg acgagctgct ggtggcccgg cgcgagcgta
     5221 tggtcctcaa ggagggcggc tcggccggcg gcaagggggt cttcctcgga aagttcacgc
     5281 cggagccgga gtggcgggag gccatcgcca ccgcgatgca gagccgcggc caatgggtcg
     5341 tccaggagca tttcgagtcg ctgccctacc tctatcagag cggcgatcac ggagcctcgg
     5401 tgcacgacgt catctggggg cccttcgtgt tcggcggcga gtacgccggg gtgatcctga
     5461 ggatgcagcc ccgtgccgcc gggggagccg tcaacctgag cctcgccgcg accgaagggg
     5521 tggtcttcga ggtctgagga cgcccttctt ccaccgagcg cgccgtggcc gttccccgcc
     5581 cagcacacct tgacatccta ggcatccctt cctaccatgc tatggcatcc taggcatcgc
     5641 gccagggcca ccatcggagg taggacaggt gaagagagag aagaaggcgg acctgctgca
     5701 gggtacgctc gacatgttga tcctcaaggc cgtttccctc ggcccgctgc acggctacgg
     5761 catcatccag cgcatccacc agatgtccgg cgagatgttg caggtggaac agggctcact
     5821 gtatccagcg ctctatcgca tcgagcagaa gggctgggtc acctctcaat gggaggtcaa
     5881 cgagaccggc cgccgtgcca agttctactc gatcaccaga gccggtgcga gccagctcga
     5941 ggccgaagag gcgagctggg accgcctcgt cctggccatc gccaaagtcc ggcaggccac
     6001 gtaggaggtg ccgacgatgc gatggctcaa tgtattttcg gcccgcgtgc gcgcgctgtc
     6061 cgaccgcgag gcggtcctgc acgacatcga cgaggagatg cgcctccatc tcgagatggc
     6121 gaccgaggcg aacgtccaga gggggatggc gccggccgag gcgcgccgcg cggcgctcag
     6181 cagcttcggc catctggaca acctccgcga cacggcgtac ggggtgcggg gaggcgggat
     6241 cgccgagacc ctctggcagg acatccggta cggcgtgcgg gtgctcgcca aacagaaagg
     6301 ttataccgcg gtcgcggtgc tcaccctggc gctggggatc ggcgccaaca cctcgatctt
     6361 ctcggtcgtc cacgagctgc tcgtgcgccc gctcccctac gcggacccgg agcggatcgt
     6421 cacggtgtgg gagctcacgc cggagggccg cgagcacaac gtggcctcgg ccgccaactt
     6481 cctgagctgg agcgggcaga gcacgagctt cgagagcatg gcggcgttct cggaccagcg
     6541 ggtgaacctg accg
//
LOCUS       DASPJI010000085         7996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_749118, whole genome shotgun sequence.
ACCESSION   DASPJI010000085 DASPJI010000000
VERSION     DASPJI010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7996)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749118"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VEL74_02240"
     CDS             <1..191
                     /locus_tag="VEL74_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61376.1"
                     /translation="ETVARMRDDFVSILSRAAAQPHLPLSALTEPLAERARQEQILRQ
                     RQRKETYIGKLKKLSRSA"
     gene            208..7437
                     /locus_tag="VEL74_02245"
     CDS             208..7437
                     /locus_tag="VEL74_02245"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61377.1"
                     /translation="MLEEMTETVTPAAETLQGFELSPQQRHLWTLLHDGAGASGLADS
                     PYRARAIVAIEGDVESARLREALDRVVRRHEILRMGFHRLPGMTTPLQVPREAGFSWR
                     ALDGGGCERERIAALWEQPAGAAETPLSASWAPLSAERSLLLLNLPALCCDARGLDLL
                     VDEVSRAYAGQYEAPAEEVGYVDLAAWQNELLLSPDTEKERLHWLLQAPAATESPRLP
                     FEGRARGGGFSPRTLSLLADDGLGMRVETAAAACGVPASIVLLACWKILLWRLTGGGR
                     IVVGVAHDGRNYEELATCPGLFVKHLPIVSELEPGFRFSELVLRLHAASETAAEMQQF
                     FSWDLLEGVGGHRFPAFCFELTGDPEPTEPVAPRFSVMHRRVLAERFVVKLACRQGGG
                     AGAGLVLELQYDASRLDATAAGRMAAQLEEILVRALSTPGDPISELPALAVHERHDLL
                     YERNDTRIELGAGFGLHELVARQARETPGEVAAELEGKSLTYSELVERAWRLSRRLRI
                     MGVGPDVPVAVCLDRSLDMLVALLGVLGAGGAYVPLDPAYPRERLELMIEDSGAPLLL
                     THQALAVSLPEQAARVVCLDAADGEVQDEVEDGRVPVAVSPQNLAYVIYTSGSTGRPK
                     GVMISHGAINNRLLWMCRRFGLSGGERVLQKTSFSFDASLWELFMPLLRGSTVVLARP
                     GGQQDSAYLAKAVAGERITILQLVPSMLRIFLEEPGLSGCRSLRRVFCGGEVLPAELR
                     DRLLARLDAQLVNLYGPTEVAIDATSWVCPPGEAVVPVGTPLDNVEVYLLDGGLEPVP
                     SGAVGEIYVGGAGLARCYLGQPERTAERFVPSPFGQPGSRLYRTGDLGRHLADRGIEF
                     LGRADHQVKVRGFRIELGEIEAALGAHPAVRQAVVTAREDSPGDQRLVAYVVTADPGE
                     AASAELRRWLAERLPEHMIPAAFVRIDRLPLLPNGKLDRGALPAAGTERSEGEYAPPR
                     TPTEEMLAAIWGELLGVERAGARDSFFELGGHSLLATQLVSRLRQSMGVEVGLRAIFE
                     TPVLAALAWRIDQLRRGDYLPEAPPLVPVPRDGALPLSFAQQRLWLLDRLDPWSAAYN
                     LPLSLWLSGWLRVPLLEHSLSEIVRRHESLRTVFVEIDGEPVQKVLPHRPCSLPVVDL
                     EALPEERRQTVALSLAWAEAQRPFDLSRGPLLRACLVRLEEREHLALATMHHIVSDAW
                     STGILIRELSTLYQSLADRTAPALPELPVQYADFAVWQRGWLQGEVLKEQLAYWRGQL
                     GGDLPVLQLPTDRPRPPVQTVRGAVLTARLPLALTEDLERLSRAEGTTLFMTLLAGFQ
                     ALLGRYSGQRDVIVGSPIANRTRLEVESLIGFFVNTLALRGDLGEEGTFRDLLSRTRE
                     AVLGASAHQDLPFEKLVEELQPRRDLSRSPLFDVLFVLQNAPAERLALSGLMLRPLGV
                     DSGRSPFDWTVSMTRTDQGLATSFEYNRDLFDRGTVTRALVHLESLLEAWAADPGAGL
                     WETPLLSPAEEHQVRVEWNRLGESFPEVSLPDLVAAQVQSRPDASAAVFENQVWSYAG
                     LDRQAERVARRLTAAGVGAGDLVGIFAERSLEMLAALLGVLRLGAAYVPLDPGQPRER
                     LELMLEEAGLVAVLALGDVTASLPGTAPRVIQWNGDGEERNARREGVGQRSDGDAPAY
                     VIYTSGSTGRPKGVQVTHRALVNFLWSMKEEPGLRAEDVWLSVTPLSFDIAGLELYLP
                     LIVGGRVVLASRETAMDGRRLLALLRDSEATVLQATPASWRLLLAADWEGTPGLTALC
                     GGEALPRRLAADLCERVGALWNVYGPTETTIWSTLARVEPEEGPISIGRPLANTQVHL
                     LDTDWRPVPVGAAGELCIGGTGLAVGYLRRPDLTAERFIPDPLGPVPGARLYRTGDLA
                     RFWSDGRLEYLGRQDHQIKLRGFRIELEEIEAVLGRHPLLRQVVVRPRQDATGEPRLV
                     AWIVAAEGIAAPSASDLRVYLHDKLPEPMVPAAFVALDALPLTPNGKVDTKALPEPDA
                     KRLLGAAPRDLLELKLLEIWKGLFSQAEIGITDDFFELGGHSLLGVRLMALIRRRFER
                     ELPLATLFKHPTVGGLAGVLRRQASDAPWSPLVTIQPSGSGAPFFCVHPIGGQVLCYA
                     GLARHLGTGRPFYGIQAPPLEQDAGFLHTTVEGMAAAYIDVLRSVQETGPYLIGGASF
                     GGLVAYEMAQQLVRQGEEVALLALLDTIRPVHNRRITESDYAAELAGLVREYASERGR
                     DDLSFSAQDLLPLDREGQLQLILETLRAFDLLPAEIDLDWLRRFVDGHLSRLEARLSY
                     EPAPYPGRITLFKARDQDSRYLEELSIDARADVLEPTLGWSALCAGRVDVLQVPGYHA
                     NIFLEPHVRVLARELGRTIDETLGAGASVTAPASTLAGSVLDKES"
     gene            7441..>7996
                     /locus_tag="VEL74_02250"
     CDS             7441..>7996
                     /locus_tag="VEL74_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG61378.1"
                     /translation="MSDLLQDIRYGLRAIRKSPGFAAAVLLTLMIGIGANSAIFSVID
                     AVLLRPLPYSDPQRLVVAWTQFLQVGISENYFSIAEFQDLMKQNRSFERVAAYRSTVN
                     LNLTGVAEPERLPSAMVTSDFFPILDVDAQAGRTFLQEDADSDRDVVVLSHGLWQRRF
                     GSDPGVVGRTMSLDGKANVIVGVMP"
BASE COUNT         1109 a         2716 c         2816 g         1355 t
ORIGIN      
        1 ccgagacggt ggcgcggatg cgggacgact tcgtcagcat tctgtcccgg gccgctgctc
       61 agccgcatct cccgctgagc gcactgaccg agcctctcgc cgagagggcg cgccaggagc
      121 agatcctgcg ccagaggcag cgcaaggaga cctacattgg caagctgaag aagctcagcc
      181 gtagcgcttg aacgcaggac cgaaccgatg ctcgaagaga tgacggaaac cgtgacgccg
      241 gcggccgaga ccctgcaagg gttcgagctg tcccctcaac agaggcacct ctggaccctc
      301 ctccacgacg gagcgggtgc cagcggcctt gctgacagcc cctatcgcgc ccgggccatc
      361 gtggcgatcg aaggggacgt ggagtccgcg cggctccgcg aggccctgga ccgggtggtg
      421 cggcgccacg agatcctgcg gatgggcttc caccgcctgc ccgggatgac cacgccgctg
      481 caggtgcccc gggaggcggg cttctcctgg agagcgctcg acggcggcgg ctgcgagcgc
      541 gagcggatcg cggcgctgtg ggagcagccc gcgggcgcgg ccgagacgcc cctgtcggca
      601 tcgtgggcgc cgctctccgc ggagcggtcg ctgctgctcc tcaacctccc agccctctgc
      661 tgcgacgcac gggggctgga cctcctcgtg gacgaggtaa gccgcgccta tgcggggcag
      721 tacgaagcac cggcggagga agtgggttat gtcgacctcg ccgcctggca gaacgagctg
      781 ctgttgtccc ccgatacgga aaaggagagg ctccactggc tccttcaggc accggccgcc
      841 actgagtccc cccgcctgcc cttcgagggg cgagcccgcg gcggcgggtt ctctcctcgc
      901 acactgtcgc tgctggccga cgacggcctg gggatgagag tcgagacggc cgccgccgct
      961 tgcggcgttc cggccagcat cgtcctgctg gcttgctgga agatcctgct ctggcgtctg
     1021 accggcggcg gcaggatcgt ggtaggggtg gctcacgacg gccggaacta cgaggagctc
     1081 gccacctgcc caggcctttt cgtcaagcat ctccccatcg tctcggagct ggagcctggg
     1141 tttcgcttca gcgaactggt gcttcgcctc cacgctgcgt cggagactgc cgcggagatg
     1201 cagcagttct tcagctggga tcttttggag ggcgtcggcg gccaccggtt cccggccttc
     1261 tgtttcgagc tcaccggaga tccggagcca acggagcccg tcgcgccacg cttttcggtg
     1321 atgcaccgcc gcgtactggc cgaacgattc gtcgtcaagc tcgcctgccg ccaaggcggg
     1381 ggagcagggg cgggtctggt gctggagctc cagtacgatg cctcccgcct ggacgcgacg
     1441 gcggccgggc gtatggcggc gcagctcgag gagatcctcg tgcgtgccct gagcacgccc
     1501 ggggacccca tcagcgagct gccggccctc gctgtccacg agcggcacga tcttctatac
     1561 gagcgcaacg acacccggat cgagctgggc gccgggttcg gtctccacga gctggtggcg
     1621 cgccaagccc gggagacgcc gggggaggtg gccgctgagc tcgaaggcaa gtccctcact
     1681 tacagcgagc tcgtcgagcg tgcctggcgc ctgtcccgcc gcctgcgcat catgggggtc
     1741 gggcccgatg tgccggtggc ggtctgcctc gaccggtcgc tcgacatgct ggtggcgctc
     1801 ctcggcgtcc tcggcgccgg cggtgcctac gtgcccctcg atcccgccta cccgcgcgag
     1861 cggctcgagc tgatgatcga ggacagcggc gcgcccctgc tcttgaccca ccaggcgctg
     1921 gccgtcagcc ttccggagca ggcggcgaga gtcgtctgtc tcgacgccgc ggacggcgag
     1981 gtccaggacg aggtggagga tggccgcgtg ccggtagcgg tctcgccgca gaacctcgcg
     2041 tacgtgatct acacctcggg ttccaccggg cggcccaagg gcgtcatgat ctcccacggc
     2101 gcgatcaaca accgtctgct ctggatgtgc cggcggttcg gcctgagtgg cggcgaacgg
     2161 gtgctgcaga agacctcgtt cagcttcgac gcctccctgt gggagctgtt catgccgctg
     2221 ctccgcggct cgaccgttgt gctggcgcgg cccggagggc agcaggacag cgcctaccta
     2281 gccaaggcgg tggccgggga gcggatcacc atcctgcaac tggtgccctc catgctgcgc
     2341 atcttccttg aggagcccgg gctctccggc tgccgctcgc tccggcgggt cttctgcggc
     2401 ggcgaggtgc tgccggcgga gctccgggac cggctcctcg cccgcctcga cgcgcagctg
     2461 gtgaacctct acggtcctac ggaggtggcg atcgatgcca cgtcctgggt ctgccctcca
     2521 ggcgaggcgg tggtgcccgt cggtactccc ctcgacaatg tcgaggtcta tctgctggac
     2581 ggtggcctag agccggtgcc gtcgggcgcg gtgggggaga tctacgtcgg tggcgccggt
     2641 ctggcccgct gctacctggg acagcccgag cgcaccgccg agcgcttcgt cccctcccca
     2701 ttcggccagc caggatcgcg cctctaccgt accggcgacc ttggaaggca tctggcagac
     2761 cgcggcatcg agttcctggg ccgggcggat caccaagtca aggtgcgggg ctttcgaatc
     2821 gagctgggcg agatcgaggc cgccctggga gcgcatcccg ccgtgcggca ggccgtggtg
     2881 accgcgcgcg aggacagccc gggagaccag cgcctggtcg cctacgtcgt gacggcggat
     2941 cccggcgagg cggcttccgc ggagctgcgg cgctggctcg cggagcggtt gcccgagcac
     3001 atgatccccg ccgcctttgt caggatcgac agactacccc tgctgcccaa cggcaagctg
     3061 gaccgcggcg ccctgccggc tgccggcacc gagagatcgg aaggcgagta cgcgccgccg
     3121 cgcacgccga ccgaggagat gctcgccgca atctggggcg agttgctggg agtggagcgc
     3181 gccggggcgc gggacagctt cttcgagctg gggggccact ccctgctggc gacacaactg
     3241 gtgtcgcgcc tgcgccagag catgggcgtg gaagtggggt tgcgggcgat cttcgaaacg
     3301 ccggtgctgg cggcgctggc ctggcgcatc gaccagctcc gccgcggcga ttacctgccg
     3361 gaggccccgc ccctggtgcc agtcccgcgg gatggcgccc tgccgctgtc cttcgcccag
     3421 cagcgcctct ggctgctcga ccggctcgat ccgtggagcg ccgcctacaa ccttcccctg
     3481 agcctgtggc tgagcggctg gctgcgagtc ccgctattgg agcacagcct gtccgagatc
     3541 gtccggcgcc acgagtcgtt gcgcacggtg tttgtcgaga tcgacggtga gccggtgcag
     3601 aaggtcttgc cgcaccgtcc ctgtagcttg ccggtggtgg atttggaggc cttaccggag
     3661 gagcggcgcc agaccgtggc gctctccctg gcgtgggcgg aggcccagcg ccccttcgac
     3721 ctctcccgcg ggccgctgct gcgcgcctgc ctggtgcgtc tcgaggagcg cgagcacctg
     3781 gccttggcca ccatgcatca catcgtgtcc gacgcttggt ccacgggcat cctgatccgt
     3841 gagctgtcga ccctctatca gagcttggcg gatcgaacgg caccggcact gccggagctg
     3901 ccggtgcagt acgccgactt cgccgtctgg cagcggggct ggttgcaggg cgaggtcttg
     3961 aaggagcaac tcgcctactg gcgtggtcag ctcggcggcg acttgccggt gctgcagttg
     4021 ccgacggaca ggccgcgccc gccggtgcag acggtacgcg gcgcggtgtt gacggcgagg
     4081 ttgccgctgg cgctgactga ggatctcgag cggctgagcc gtgccgaggg gacgacactc
     4141 ttcatgaccc tcctcgccgg cttccaggcg ctcctcggtc gctatagcgg tcaacgggac
     4201 gtgatcgtgg gctcgcccat cgccaaccgt acgcgcctcg aagtcgagag cctgatcggg
     4261 ttcttcgtca acaccctggc gttgcgggga gacctgggcg aagaggggac cttccgcgat
     4321 ctcctgtcgc ggacgcggga ggccgtcctg ggggcgtccg cccatcagga cctgccgttc
     4381 gagaagctgg tggaggagct gcagccgcgc cgcgacctaa gccgctcgcc cctgttcgac
     4441 gtgctcttcg tcctgcagaa cgcgccggcc gagcggctgg ccctgtcggg actgatgctg
     4501 cgcccgctgg gggtggacag cggccgctcg ccgtttgact ggaccgtgtc gatgacgcgg
     4561 acggaccagg gactcgccac ctcctttgag tacaaccggg atctctttga ccgcggcaca
     4621 gtaacccggg cgctggtgca tctcgagagc cttctggagg catgggccgc tgaccccggt
     4681 gccggcctct gggagacccc gctcctgtcg ccggccgaag agcaccaggt ccgcgttgag
     4741 tggaaccgtc tgggagagtc cttcccggag gtaagcttgc cggatctcgt cgctgcgcag
     4801 gtccagagcc ggcccgatgc cagcgcggcg gtcttcgaga accaggtctg gagctacgcc
     4861 ggtctcgacc gtcaggctga acgggtggcc cgccgcctca cggccgcagg ggtcggggcg
     4921 ggggacttgg tggggatctt cgctgagcgc tccctcgaga tgctggcggc gctactgggt
     4981 gtgctgcgtc tgggtgcggc ctacgtgccc ctagacccgg ggcagccgcg ggagcgcttg
     5041 gagctcatgc tcgaggaggc cgggctcgtg gcggtgctgg cgcttggaga cgttaccgca
     5101 tccctgccgg gaacggcgcc gcgggtgatc cagtggaacg gtgacgggga ggagcggaac
     5161 gcgcgacggg agggggtggg gcagaggtcg gatggcgatg cgcctgccta cgtgatctac
     5221 acctccggtt ccaccggccg gcccaagggg gtccaggtga cgcaccgcgc cctggtgaac
     5281 ttcctctggt cgatgaagga ggagccgggc ctccgggcgg aggacgtctg gctctccgtg
     5341 acccccctgt cgttcgacat cgccgggctc gagctctacc ttcccctgat tgtcggtggc
     5401 cgtgtggtcc tggcaagccg ggagacggcc atggacggcc ggcgcctgct cgcattgcta
     5461 cgggattcgg aggccaccgt cctgcaggcg acgccggcca gttggcgtct gctcctcgcg
     5521 gcggactggg aggggactcc gggccttacc gcgctctgtg gcggtgaagc cctgccgcgg
     5581 cgtctggcgg cggacctctg cgagcgcgtc ggcgcgctct ggaacgtgta cggtccgacc
     5641 gagaccacca tctggtcgac gctggcacgg gtcgagccgg aggaggggcc catctccatc
     5701 ggccgtcccc tggccaatac gcaggtccac ctcctggaca cggactggcg tccggtcccc
     5761 gtgggcgccg cgggagagct ctgcatcggc ggaaccggtc tcgccgtggg ctacctgcgg
     5821 cgtcccgacc tgacggcgga gaggttcatt cccgatcctc tcggtccggt gccaggggca
     5881 cgcctctacc gaaccggcga cttggcgcgc ttctggagcg acggccggct ggagtacctc
     5941 ggcaggcagg atcaccagat caagctacgg ggtttccgta tcgagctcga ggagatcgag
     6001 gcggtgctcg gccgccatcc attgctccgg caggtggtag tgcggccgcg ccaggacgcg
     6061 acgggggagc cccggctggt agcctggatc gtggcggcgg agggaatcgc ggcgccttcg
     6121 gcctccgatc ttcgggtcta cctgcacgac aagctgcccg agcccatggt gccggccgcc
     6181 ttcgttgctc tcgacgccct gccgctcact cccaacggca aggtcgatac caaggcgctg
     6241 cccgagcccg acgccaaacg cctccttggc gcggcgcccc gggacctctt ggagctcaag
     6301 ctactggaga tctggaaggg cctcttcagc caggcggaga tcggcatcac cgacgacttc
     6361 ttcgagttgg gaggacattc gctcctgggt gtacggctca tggcgctgat ccgccggcgc
     6421 ttcgagcgcg agctgcccct cgccactctg ttcaagcacc ccacggtggg cgggttggcg
     6481 ggggtgttgc gccgccaggc ttcagatgcc ccctggtcgc ctctcgtgac catccagccc
     6541 tcgggctcgg gcgcgccgtt cttctgtgtc catcccatcg gtgggcaggt gctctgctac
     6601 gccgggctcg cccgccacct cggcaccggc cgccccttct acgggattca ggccccgccc
     6661 ctggagcagg atgcgggatt cctccacacg acggtcgagg gcatggcggc ggcgtacatc
     6721 gatgtcctcc gatcggtgca ggagaccggg ccctatctga tcggtggagc ctccttcggt
     6781 ggcctggtgg cctacgagat ggcgcaacag ctcgtccgcc agggagagga ggtcgccctg
     6841 ctcgcgctgc tcgacaccat ccggcccgtc cacaaccgcc ggatcaccga gtctgactac
     6901 gccgcggagc tggcgggcct ggtccgtgag tacgcgtccg agcgaggccg tgacgatctg
     6961 tccttctcgg cgcaggacct cctgccgctg gaccgtgaag ggcaactcca actgatcctg
     7021 gagacgctcc gcgccttcga cctgctcccg gcggagatcg acctggactg gctgcgccgg
     7081 ttcgtcgatg gccatctgtc ccggctagag gcccgcctct cctatgagcc ggcaccgtat
     7141 ccagggagga tcacactctt caaggcgcgc gaccaggact cccggtatct cgaagagctc
     7201 tccattgatg cgcgcgcgga tgtgttggag cccacactcg gctggtcggc cctgtgcgca
     7261 ggtcgtgtcg acgtcctcca agtccccggg tatcatgcca acatcttcct cgagccgcat
     7321 gtccgggtcc tggcgcggga gcttggacgg actatcgacg aaactctcgg agccggtgcg
     7381 tcggtcacgg cgccggcgtc cacgctcgcc ggaagcgtcc tcgacaagga gtcttgattc
     7441 atgagcgatc tgctgcagga cattcgttac ggactccgcg ccatccggaa gagccccggg
     7501 ttcgcggcgg ccgtcttgct caccttgatg atcggtatcg gtgccaacag cgccatcttc
     7561 agcgtcatcg acgcggtctt gctgcgcccg ctgccctatt cggacccgca gcgcctcgtc
     7621 gtggcctgga cccagttctt gcaggtcggg atctcggaaa actacttttc cattgccgag
     7681 tttcaagacc tgatgaagca gaaccgttcg ttcgagcggg tcgcggcgta tcgcagcacc
     7741 gtgaacctca acctcacggg cgttgccgag cccgagcggc tgccgtcggc catggtcacg
     7801 tcggacttct tcccgatcct tgatgtcgac gcccaggccg gccgcacgtt cctccaggag
     7861 gatgcggaca gtgaccgaga cgtggtggtg ctcagccatg gtttatggca gcggcggttc
     7921 ggttctgatc ccggggtggt cgggcgcacc atgtcgctgg acggcaaggc gaacgttatc
     7981 gtcggagtca tgccgg
//
LOCUS       DASPJI010000086         6247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_576981, whole genome shotgun sequence.
ACCESSION   DASPJI010000086 DASPJI010000000
VERSION     DASPJI010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576981"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..755)
                     /locus_tag="VEL74_02255"
     CDS             complement(258..755)
                     /locus_tag="VEL74_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61379.1"
                     /translation="MRSDDDRNTPRVPNIFLREFLDRLGERLEPPSAAEAAVAGTWRL
                     EEIRATFYAVFRPETPPARGYRPTAVFFDPHHALLIAALLPGTGRDPLYRLHKEPTAD
                     GYPVESGGEVLGHLAVFDDKLIDALHVVECILRSPEALANLLEAAGAVVLERAGAILE
                     GRMGE"
     gene            complement(1035..1250)
                     /locus_tag="VEL74_02260"
     CDS             complement(1035..1250)
                     /locus_tag="VEL74_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HYG61380.1"
                     /translation="MDKDDLIRRALELPLEEQLDVAQALWSQASPPPDGILSDDLQTL
                     LEARLLEARQNPEAGVTWEEMKVRLGW"
     gene            complement(1255..2454)
                     /locus_tag="VEL74_02265"
     CDS             complement(1255..2454)
                     /locus_tag="VEL74_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HYG61381.1"
                     /translation="MPSTPLRLSRRLPWDAPSNRLTRAMAERSASGLPTLDLTETNPT
                     RVGLPYPEEELAELLGRAGRPDYPPHPRGLPEAREALAAALSRPGDPVEPDDLLLTAS
                     TSESYSFLFKLFADPGDEVLTAVPSYPLLDSLAALDGLELRHVHSAPGRRLDVETVDL
                     ALTERTRLLAVVHPGNPAGRFFTPPEQQALATLCAARGLPLISDEVFADYPLADLPPA
                     ALPAASLDAEVLTFSLGGLSKSAGLPSWKLGWIRAGGPRDLRRQALAALELVADSYLS
                     VATPVQRALPELLALVPRLRALILDRLRANLATLRDALAGHPTLHLLEPKGGWSAVLR
                     VALPIADEDLALELLEHAGVLVHPGYFFDFATDDYLVVSLLPEPGVFAEGVGRLGERL
                     ASGAGAQ"
     gene            2555..3346
                     /locus_tag="VEL74_02270"
     CDS             2555..3346
                     /locus_tag="VEL74_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HYG61382.1"
                     /translation="MSTALPDLETVCATRYVTPLREGGSLPAIVEASDSGMYVLKFRG
                     AGQGPLALVAELIAGQVGRALGLKVPELVLMEVDTALGRNEPDPEIRELLRASVGINL
                     GVDYLPGSVTFDTAADRAMADIASETVWFDAFITNVDRTPRNPNLLWWHKVLYLIDHG
                     AALYFHHNWPSREAMAASRFAAIRDHVLLPWASRLEEADQRLRPLLGEDLFARVVAQI
                     PEDWLMPEPGLSADDRRAGYVELLTKRLAAASFLEEAVRAHAQLV"
     gene            3324..3713
                     /locus_tag="VEL74_02275"
     CDS             3324..3713
                     /locus_tag="VEL74_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HYG61383.1"
                     /translation="MPTRSSYDYAVIRVVPRVERGELVNAGVILFCLEKDFLQARVEV
                     DEPRLRALWPGIDVELVRQHLEAIPRICAGSPGAGPIGKLSLRERFHWLVAPRSTIIQ
                     VSPVHAGLCDHPERAIEDLFQKVVLVS"
     gene            3749..4570
                     /locus_tag="VEL74_02280"
     CDS             3749..4570
                     /locus_tag="VEL74_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017742662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HYG61384.1"
                     /translation="MRLTDDQIRTYEEEGWLLLPGCFSAAEVGTLKAQLSAEFAADSE
                     RRVLEKGSTIVRSVYGSHAVNEVFGRLVRIPRLLEPARQLLGGDVYVHQFKINAKAAF
                     GGDVWEWHQDYIFWLREDAIPEPRLVNVVVFFDEVNEFNGPMFMVDGTHKEGVLSSRT
                     LEKPPEGYGDSPAWISNLTADLKYSIGKEIVAEMVGRHSLSSAKGPAGSVLFFHPNLL
                     HGSTPNMSPFDRQIAIVTYNSVENPPGPVEKRRPWFLVSESCEALTPLPDDALAG"
     gene            4652..5749
                     /locus_tag="VEL74_02285"
     CDS             4652..5749
                     /locus_tag="VEL74_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HYG61385.1"
                     /translation="MTSAEDWTIEEDIRRASTLPGRVYSDPHWFALARERVFARSWQL
                     VGDTDSVRVPGQVFPFTLLEGVLDEPLVLTRDGSDTVHCLSNVCTHRGTILCEGAGVE
                     QVFRCRYHGRRFSLDGRFLSMPEFGEVEGFPSERDHLARVPLGRWGKFLFASLDPAFP
                     LAELIAEMDARVGWLPLRDAVLDPARSRDYLVRANWALYCDNYLEGFHIPYVHSGLNA
                     ALDYGEYRTELYSWANLQLGVASGGDDDVFDLPASSPDAGQRIAAYYYWLFPNTMFNV
                     YPWGISVNVVRPLAPDRTRVSFLSYVWDPSRLDRGAGTGLDRVEREDEAVVEGVQRGV
                     RSRLYHRGRYSPTREQGVHHFHRLLERFLRG"
     gene            5742..>6247
                     /locus_tag="VEL74_02290"
     CDS             5742..>6247
                     /locus_tag="VEL74_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HYG61386.1"
                     /translation="MDRRPELIRGLGTWDGALLTIGSVVGTGIFLTTSDMARVLPRPG
                     LILLVWLVGGLLTLAGALTYAELGVMFPRAGGLYHFIKETYGPLWGFLYGWTAFLVIM
                     SGGIAALGVAFGEYLGSFLPWFSTGHILLNVPIGGWSWTVSGGQVAAALAILLLTGIN
                     HLGLKEGAG"
BASE COUNT          928 a         2071 c         2255 g          993 t
ORIGIN      
        1 gcgcctcgat cagattggag aacgacagcc tggagtccct gagctcgggc ctgtggatga
       61 gcgcctccca acgccgcgca ccccccgcca ctggatagga gcggccggcc acccaactct
      121 tgagggtgtt ctccgacatc ctgagatagc gtgccgcctc agggagcgag taacggggca
      181 attcgttcga atccacggct tcattgtaaa acatccggcg gccggctctc gccggccgcc
      241 gggttccccc tacctctcta ctcccccatc cgcccctcca gaatcgcccc cgcccgctcc
      301 aggaccaccg cgcccgctgc ttccagcagg ttcgccagcg cttccggtga cctcaggatg
      361 cactcgacga cgtgcagggc gtcgatgagc ttgtcgtcga agacggcgag gtggccgagg
      421 acctctcctc cggactcgac ggggtagccg tcggcggtgg gttccttgtg gagccggtag
      481 aggggatcgc ggccggtgcc ggggaggagg gcggcgatca gcagggcgtg gtgcgggtcg
      541 aagaagaccg ccgtgggacg gtagccccgg gcgggcggcg tctccggacg gaagacggcg
      601 tagaaggtgg cccggatctc ctctaggcgc caagtcccgg ccaccgccgc ctccgcggcc
      661 gagggcggct ccaggcgctc gccgagacgg tccaggaact cgcgcaggaa aatgttgggc
      721 acacgggggg tgttacgatc gtcatcgctt cgcattggat ctaactcctt tgtgaagtag
      781 gtccgggggg gagttgtcgg ctcgctcccg ggccgcttcc atctgtgttg gccgaagccg
      841 gaacgcccgg atcggcggcg aaacaacaaa cggcacagcg acttctacga cgggtacgga
      901 tcaggctggg tcccttcggg tccggatacc tcctttcctc gggacgcgat ggcttcgcgc
      961 cagagcgcta tggggaatta tatggaagag gacccgacaa agcaagcgaa aggtgaagat
     1021 cggcacgatt gatcctacca accgagccgg accttcatct cctcccaggt cacccccgct
     1081 tcagggttct ggcgggcttc gagcaaccga gcctccagta acgtctggag gtcatcagag
     1141 agtatgccgt cagggggcgg cgaggcctgg gaccagagag cctgggctac atcgagttgc
     1201 tcctcgagcg gcagctccaa tgcgcgtcgg atcagatcat ccttatccat aggtctattg
     1261 tgctcctgct ccgctcgcga ggcgctcccc caatctcccc accccctccg cgaagacccc
     1321 cggctcgggg agcagggaga cgaccaggta gtcgtcggtc gcgaagtcga agaagtagcc
     1381 cggatggacg aggacgccgg catgctccaa gagttccagg gccaggtctt cgtccgcaat
     1441 gggtagagcg acgcggagga cggcggacca gccccctttg ggttcgagga ggtggagggt
     1501 cgggtggccg gctagggcgt ctcggagcgt cgcgaggttg gcgcgcaggc ggtccaggat
     1561 cagggcgcgg aggcggggaa ccagggcgag cagctcgggg agtgcgcgct ggaccggggt
     1621 cgcgaccgag aggtagctgt ctgcgaccag ctccagggcg gcgagggctt ggcggcggag
     1681 gtcgcggggg ccgccggcgc ggatccagcc cagcttccag gaggggaggc cggcgctctt
     1741 cgagaggcct cctaatgaaa aggtaagaac ttcagcgtcc agggatgcgg cggggagggc
     1801 ggccgggggc aggtcggcga gagggtagtc ggcgaagacc tcgtccgaga tcagcggcag
     1861 gcctcgggcg gcgcagaggg tggcgagggc ctgctgctcc ggcggggtga agaagcggcc
     1921 ggccgggttg cccgggtgga cgacggcgag gaggcgcgtg cgctcggtga gggcgaggtc
     1981 caccgtctcg acgtccaggc gccggccagg ggcgctgtgg acgtgccgca gctccagacc
     2041 gtcgagcgcc gccaggctgt cgagcagagg ataggacggc accgccgtca gcacctcgtc
     2101 ccccgggtcg gcgaagagct tgaagagaaa ggagtaggac tcgctggtcg aggcggtcag
     2161 caggaggtcg tccggctcga cggggtcccc gggacgggac agggcggcgg ccagggcctc
     2221 gcgcgcctcg ggcagtcccc tggggtgcgg cggatagtcc ggcctcccgg cgcgcccgag
     2281 cagctccgcc agctcctcct cgggatacgg cagccccacc cgtgtcgggt tcgtctctgt
     2341 cagatcgagg gtggggagcc cggaggccga gcgctccgcc atcgcccggg tcaggcggtt
     2401 ggagggggcg tcccagggga ggcggcggga gaggcggagg ggggtggagg gcatgcgggg
     2461 agtctagcgc cctcaccccc cgaccccctc tcccgcctcc ccacccccct ccccgggaga
     2521 gggggaggcg atctcgctaa ggtaagatcc cgccgtgtcc accgccctgc ccgacctcga
     2581 gaccgtctgc gcgacccgct atgtgacccc gctgcgggag gggggctcgc tgcccgccat
     2641 cgtcgaggcg agcgactcgg ggatgtatgt gctcaagttc cggggggcgg ggcaggggcc
     2701 tctcgcgctg gtggccgagc tgatcgccgg ccaggtcggg cgggcgctcg ggctcaaggt
     2761 gcccgagctg gtgctgatgg aggtggatac ggcgctcgga cgcaacgagc cggacccgga
     2821 gatccgcgag ctgctccgcg ccagcgtcgg gatcaacctg ggggtcgatt acctgccggg
     2881 gtctgtgacg ttcgacacgg cggccgaccg cgccatggcc gacatcgcct cggagacggt
     2941 ctggttcgac gcgttcatca ccaacgtcga ccggacgccg cgcaacccca acctgctctg
     3001 gtggcacaag gtcctctatc tcatcgatca cggcgcggcc ctctacttcc atcacaactg
     3061 gccgagccgg gaggccatgg ccgccagccg gttcgccgcc atccgcgacc atgttctcct
     3121 cccctgggcg agccgcctgg aggaggccga ccagaggctg cgcccgctgc tcggcgagga
     3181 tctgttcgcc cgcgtcgtgg cccagatccc ggaggactgg ctgatgcccg agccggggct
     3241 ctcggcggac gaccggcgcg ccggttatgt ggagctcctg acgaagcggc tcgccgcggc
     3301 gtccttcctg gaggaggcgg tccgtgccca cgcgcagctc gtatgactac gccgtgatcc
     3361 gggtggtgcc gcgggtcgag cgcggggagc tcgtcaacgc cggggtgatc ctcttctgtc
     3421 tggagaagga cttcctccag gcccgggtcg aggtcgacga gccgcgcctg cgcgccctct
     3481 ggcccgggat cgacgtcgag ctggtccgcc agcacctgga ggcgatcccc aggatctgcg
     3541 ccggcagccc gggcgccggc cccatcggca agctctccct gcgcgagcgc ttccattggt
     3601 tggtggcgcc gcgcagcacg atcatccagg tctcccccgt gcacgcgggc ctctgcgacc
     3661 atccggagcg ggcgatcgag gatctgttcc agaaggtggt gctcgtttct taaagtacaa
     3721 gcactaagag gtaagaagga ggacgagcat gcgtttgacc gacgaccaga tcaggaccta
     3781 tgaggaggag ggatggctcc tcctgcccgg atgcttttcg gccgcggagg tcggcacgct
     3841 gaaggcgcag ctctccgccg agttcgccgc ggactcggag cggcgggtgc tcgagaaggg
     3901 gagcactatc gtgcggtcgg tctacggatc gcacgcggtc aacgaggtct tcgggcgcct
     3961 ggtccggatc ccgcgcctgc tcgagccggc ccgccagctc ctcggcggcg acgtctacgt
     4021 ccatcagttc aagatcaacg cgaaagcggc cttcgggggt gatgtctggg agtggcacca
     4081 ggactacatc ttctggttgc gggaggacgc gatcccggag ccgcgcctgg tcaacgtggt
     4141 ggtcttcttc gacgaggtca acgagttcaa cggcccgatg ttcatggtcg acggcacgca
     4201 caaggagggg gtgctgagct cccgcaccct ggagaagccg ccggaaggct acggcgacag
     4261 cccggcctgg atctcgaacc tcacggccga cctcaagtac tcgatcggca aggagatcgt
     4321 ggcggagatg gtgggccggc acagcctctc ctcggcgaag gggcccgccg gctcggtgct
     4381 gttcttccat ccgaacctgc tgcacggctc gactccgaac atgtccccct tcgaccggca
     4441 gatcgcgatc gtcacctaca acagcgtcga gaacccgccg ggaccggtgg agaagcggcg
     4501 cccctggttc ctggtgagcg agagctgcga ggcgctgacc ccgttgcccg acgacgctct
     4561 ggccggctga ggcctcctcg agaagtgggc ctctggcctt tccgtccagg aaaggcgaca
     4621 atcgccggcg atgccgccgg ccggccctcc catgacatcc gccgaagatt ggaccatcga
     4681 agaggacatc cgccgggcct cgaccctgcc ggggagggtc tactccgatc cccactggtt
     4741 cgccctggcc cgggagcggg tcttcgcccg gagctggcag ctcgtcggcg acaccgactc
     4801 cgtccgcgtg ccggggcagg tcttcccctt caccctcttg gaaggggtgc tcgacgagcc
     4861 gctcgtcctc acccgcgacg gctccgacac ggtccactgc ctgtccaacg tctgcaccca
     4921 ccgggggacg atcctgtgcg agggcgccgg ggtggagcag gtgttccggt gccgctacca
     4981 cggccggcgg ttctctctgg acggccgttt cctctcgatg cccgagttcg gcgaggtcga
     5041 gggctttccg tcggagcgcg accatctggc ccgggtcccc ctggggcgct gggggaagtt
     5101 cctgttcgcc tcgctggacc cggcgttccc cctggccgag ctgatcgccg agatggacgc
     5161 ccgggtgggt tggctccccc tgagggacgc ggtgctcgac ccggcgcgct cgcgggacta
     5221 cctggtgcgg gccaactggg cgctctactg cgacaactat ctggaggggt tccatatccc
     5281 gtacgtccac tcggggctca atgccgcgct ggactatggc gagtaccgga cggagctcta
     5341 ctcctgggcc aacctgcagc tcggggtggc gagcggcggc gacgacgacg tcttcgacct
     5401 gccggcctcg tcgccggacg cggggcagcg catcgccgcc tactactact ggctgttccc
     5461 caacaccatg ttcaacgtct atccctgggg gatctcggtg aacgtggtgc gccccctggc
     5521 accggaccgc acgcgggtct ccttcctctc ctatgtctgg gacccgtcac gcctcgaccg
     5581 cggggccggc acggggctcg accgggtgga gcgcgaggac gaggccgtgg tcgagggggt
     5641 gcagcggggc gtccgctcgc gcctctatca ccggggccgg tactccccga cgcgcgagca
     5701 gggggtgcac cacttccacc ggttgctcga acggttcctg cgtggatagg cgcccggagc
     5761 tgatccgggg gctcggcacc tgggacggcg cgctcctcac catcggctcg gtggtgggaa
     5821 cggggatctt cctcaccacg tcggacatgg cgcgggtgct cccccggccg gggctgatcc
     5881 tcctggtctg gctggtgggg ggattgctga cgctggccgg ggcgctgacc tatgccgagc
     5941 tgggggtgat gttcccgcgc gccggcggcc tctaccattt catcaaggag acctacgggc
     6001 cgctctgggg gttcctctac ggctggaccg ccttcctggt catcatgtcc ggggggatcg
     6061 ccgccctcgg ggtggccttc ggcgagtacc tggggagctt cctgccctgg ttctccacgg
     6121 ggcacatcct gctgaacgtg cccatcggcg gctggagctg gacggtgagc ggcggccagg
     6181 tggcggcggc gctggccatc ctgctcctca ccgggatcaa tcacctcggc ttgaaggagg
     6241 gggccgg
//
LOCUS       DASPJI010000087         3369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_177051, whole genome shotgun sequence.
ACCESSION   DASPJI010000087 DASPJI010000000
VERSION     DASPJI010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177051"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VEL74_02295"
     CDS             <1..700
                     /locus_tag="VEL74_02295"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYG61387.1"
                     /translation="AVRRSLYRSGVECDLLEHHDVNLNGVLPVRFDRIWQEARPVRFR
                     GAEALVMSPEDLLVSLCVNACRKRYFRLKSLFDLAESVRRLEGLDWDRLARRAREHGC
                     AAVAWTALVATRETLGCPLPEGALGGLGVPAVRRRLAGGLLSRFLRTSSLVGEPRAGL
                     SRLLPYAALRPMEAWRAFRLTVTHRPPPHHRAEVAERAAFVGGTPEASAGLSEVPAAR
                     QSFRRRAGSSGGAP"
     gene            1012..1446
                     /locus_tag="VEL74_02300"
     CDS             1012..1446
                     /locus_tag="VEL74_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HYG61388.1"
                     /translation="MSSGLPFEAVRDLAREAPVAITVRGLSMAPRLRDGDRVEIAPAR
                     FYRPGDVVAFRNADGHLIVHRLLGYRLRAGRLACVTRGDAAPLPDAPIPFANLLGRVV
                     RVAHIARRTPRQAGLVTPMDRVRSVLGFLALAAAAAGRRLRG"
     gene            1443..2399
                     /locus_tag="VEL74_02305"
     CDS             1443..2399
                     /locus_tag="VEL74_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61389.1"
                     /translation="MSFGEQFLVHPDLFPARRSGETWGERELVIDLPGGPYLFTGLNA
                     GQEAHVRRRFGSFCGLGAGSPPAVETRFFRAAEADFREIDTRGWEYGLDLDFGLAAVR
                     VAGLRLMARLDWRPGLAAAVWTPDAGGEAFAGIFENAFRALVSYRLLESGGVVLHGAA
                     LARRQEGGGAFLFLGRSGAGKSTVSRLGMERGLTVLSDDLNALLPGELPGAAVATVAA
                     ATAVVKLPFTGDVGEPGAPSPPHPLRALFRLEKSAEEAVSPLSRAEVVGCLLACSPFL
                     NTDPYRRETLVSILSALVPRSLPAYALRFSLTGRFWELPELA"
     gene            2422..2703
                     /locus_tag="VEL74_02310"
     CDS             2422..2703
                     /locus_tag="VEL74_02310"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HYG61390.1"
                     /translation="MQPLALRSILRRRADVRFRVIDREAVVVRQSAAEVVVLNEVAAR
                     ILTLADGSTSLESWVDVLAAEYEASRETLERDVAGFAAELVEQGLLEVA"
     gene            2704..>3369
                     /locus_tag="VEL74_02315"
     CDS             2704..>3369
                     /locus_tag="VEL74_02315"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYG61391.1"
                     /translation="MSFGDLLQKTWRDNILFSALIELTYRCNLDCFFCYNDLGLRGEP
                     LRTEQWFRLFDDLREMEVLNLTLSGGEPLAHPDFLRLGARARELGFVVRVKSNGHALR
                     GELARRLRDEVDPFLVEVSLHGATAAVHDRQTRVPGSFDRLLANLREMRELGLRVKVN
                     STLTAWNEGEIEGMLDLSDSLGLPLQIDPEVTPRDDGDAEPLSVSPSREGVARLFALL
                     KARA"
BASE COUNT          362 a         1268 c         1219 g          520 t
ORIGIN      
        1 ggcggtccgc cgctccctct accgcagcgg cgtcgagtgc gacctgctgg agcaccacga
       61 cgtcaacctg aacggcgtgc tgccggtccg cttcgaccgg atctggcagg aggcccggcc
      121 ggtgcgcttc cggggggccg aggccctggt catgtccccc gaggacctgc tggtctccct
      181 ctgcgtcaac gcctgccgca agcgctattt ccggttgaag agcctgttcg acctggcgga
      241 gagcgtccgc cgcctcgagg ggctggactg ggaccggctc gcccgccgcg cccgggagca
      301 cggctgcgcg gcggtggcct ggacggccct cgtcgccacc cgcgagacgc tggggtgtcc
      361 cctgcccgag ggggcgctcg gcggcctcgg cgtgccggcg gtccgccggc ggctggccgg
      421 ggggctgctc tcccgtttcc tccgcacctc ctcgctggtg ggcgagccgc gcgccggcct
      481 ctcccggctc ctcccctacg ccgccctgcg gccgatggag gcgtggcggg cgttccggct
      541 caccgtcacc caccggcccc ctccccacca ccgggccgag gtggccgagc gggccgcctt
      601 cgtcggcggc actccggaag cttccgccgg cctgtcagag gttccggcgg cgcgtcagag
      661 tttccggcgg cgcgccggaa gctccggcgg cgcgccgtag tcttcggcgc cgcgttggaa
      721 gcttccggtc ccctgtcaga ggttccggcg gctcgtcgaa ggttccgatg gcccgccgga
      781 agctctgaga gggcgtccga agcgtcggcg gctcgccgga agcttcggcg gcgcgctgga
      841 agcttacggc ggcgtgtcag agtttccgct ggcctgtcag agattccggc ggcgcgtcag
      901 agcttccgat ggctcgtcag agtttccgat ggcgcgccgt agccttcggc ggcgcgccgg
      961 agcttccggc ggcccgtcgg agcggccgga cgcctgccgt atactccgga gatgtcttcc
     1021 gggctcccct tcgaagccgt gcgggacctc gcccgcgagg cgccggtcgc catcaccgtc
     1081 cgcggtctct ccatggcgcc gcggctgcgg gacggcgacc gggtggagat cgcgccggcg
     1141 cgcttctacc ggccggggga cgtggtggcc ttcaggaacg ccgacggcca cctgatcgtg
     1201 caccggctcc tggggtatcg cctgcgcgcc gggcggctcg cctgcgtcac ccggggggat
     1261 gccgcaccgc tgccggacgc ccccatcccc ttcgcgaacc ttctgggccg cgtcgtccgg
     1321 gtcgcccaca tcgcgcgccg gacgccgcgg caggccgggc tggtcacacc gatggaccgc
     1381 gtccggtccg tcctcggatt cctcgccctc gccgccgccg cggccgggcg ccgcctgcgc
     1441 ggatgagctt cggcgagcag ttcctggtcc atccggacct cttcccggcg cggcggagcg
     1501 gcgagacctg gggggagcgc gagctggtca tcgacctgcc gggcgggccc tatctcttca
     1561 ccggcctgaa cgccggacag gaggcccacg tccggcgccg cttcggctcc ttctgcgggc
     1621 tgggggccgg ctctccgccc gcggtggaga cgcggttctt ccgcgccgcc gaggcggact
     1681 tccgggagat cgacacccgc ggctgggagt acgggctcga cctcgacttc gggctcgccg
     1741 ccgtgcgcgt cgccggcctg cggctgatgg cccgcctcga ctggcggccc ggcctcgccg
     1801 ccgccgtctg gacgccggac gcggggggcg aggccttcgc cggcatcttc gaaaacgcct
     1861 tccgcgccct ggtctcctat cgcctgctgg agagcggcgg cgtggtcctc catggcgccg
     1921 ccctggcccg tcgtcaggag ggagggggag cgttcctctt cctggggcgc tcgggggcgg
     1981 ggaagagcac cgtgtcccgg ctcgggatgg agcgcgggct gacggtgctg tcggacgacc
     2041 tcaacgccct gctccccggc gagctccccg gggccgcggt ggccacggtg gccgcggcca
     2101 ccgcggtcgt gaagctgccg ttcaccggcg acgtcggcga gccgggcgcg ccgtcgcctc
     2161 cgcatccgct gcgcgccctg ttccggctgg agaagagcgc ggaggaggcc gtctcccccc
     2221 tctcccgggc cgaggtggtg ggctgcctcc tcgcctgctc gccgttcctc aacaccgacc
     2281 cctatcgccg cgagacgctg gtgtcgatcc tctccgccct cgtcccgcgg agcctgccgg
     2341 cgtacgccct gcgcttctcc ctgaccggcc gcttctggga gctgccggag ctggcatagc
     2401 cttcgcgcta tactgttggc aatgcagcca cttgccctcc gcagcatcct gcgccgccgg
     2461 gcggacgtgc gcttccgggt catcgaccgc gaggcggtgg tggtgcgcca gagcgccgcc
     2521 gaggtcgtcg tcctcaacga ggtggcggcg cgcatcctga ccctggccga cggctccacc
     2581 tccctggaga gctgggtcga cgtcctggcc gcggagtacg aagcctcgcg cgagacgctg
     2641 gagcgggacg tggccggctt cgccgccgag ctggtggagc aggggctcct cgaggtcgcg
     2701 tagatgagct tcggagacct cctccagaag acctggcgcg acaacatcct cttctcggcc
     2761 ctgatcgagc tgacgtaccg ctgcaacctc gactgcttct tctgttacaa cgacctcggg
     2821 ctgcgcggcg agcccctgcg caccgagcag tggttccgcc tcttcgacga cctccgggag
     2881 atggaggtgc tgaacctcac cctctccgga ggcgagccgc tggcccaccc cgatttcctc
     2941 cggctggggg cgcgcgcccg ggagctcggc ttcgtggtgc gggtcaagtc gaacggccac
     3001 gccctgcgcg gggagctggc gcgccggctg cgcgacgagg tcgacccgtt cctggtggag
     3061 gtcagcctgc acggcgccac cgccgccgtc cacgaccgcc agacccgggt gcccggcagc
     3121 ttcgaccgcc tcctggccaa cctgcgggag atgcgggagc tgggtctgcg ggtgaaggtc
     3181 aacagcaccc tcaccgcctg gaacgagggg gagatcgagg gcatgctcga cctctcggac
     3241 tccctggggc tccccctcca gatcgacccc gaggtgacgc cgcgcgacga cggcgacgcc
     3301 gagccgctgt ccgtctctcc ctcacgcgag ggggtggcgc gcctgttcgc actgctgaag
     3361 gcgcgggcg
//
LOCUS       DASPJI010000088        16168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_577123, whole genome shotgun sequence.
ACCESSION   DASPJI010000088 DASPJI010000000
VERSION     DASPJI010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16168)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16168
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577123"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2162)
                     /locus_tag="VEL74_02320"
     CDS             complement(<1..2162)
                     /locus_tag="VEL74_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYG61392.1"
                     /translation="MIQEATSLQGLPLSPQQRRLWQLCPSPSFHPYRSACVVEIAGDL
                     DPGALQVALEVVARRHEILRTSFHVLPGMPYPLQVIGDALPRLASLSSGAGTPLDQLL
                     AEWQTAAADDADGPEPLRAAVACLGSGRHLLLLGLPALCGDGASLDNLTLEIARAYGR
                     GEMTDPFQYADLAAWNNEILTLEESAEGKSFWRRAAPADGLEAALPFSLRSPGAELPA
                     GFTPRTVALSFPGDLAAHASAVAEQSGAPPEVGLEAVLLAVWQLLLGLVADREELVLG
                     VVRGGRKYEEMVAALGPYARCLPLACAPGEGPFTSLVRRVGASLRELDDWQEYFDWDL
                     AAGGARAAALPFAFELTDVRPPAEAGGLTFTMARRHCWSERFEVMLRVQRYRREVTSL
                     GADLVYDAGLLGHADVERLGRWYETLLRNALAAPESRVGDLEILGGAERQRTLVEWNA
                     TGTDSGPAGTLWERVAERAARDPHGTALEWDGGEMTRAELIDRASRLASHLRRLGIGP
                     EVRVAVAVERSPEMVVSLLGVLAAGGAYVPLDPDYPQERLDRMLEDAGAAVLLTRQAL
                     AGRFPEGRFRVVCLDRLELEAGEWQGPGAAPDDLAYVIYTSGSTGRPKGVMISHRAIL
                     NRLLWMQRRFPLGADDRVLQKTPYSFDASVWEIFVPLIAGARLLLAEPGGQRDNSYLL
                     ETIARRGVTVLQLVPSQLSVFLEQEGIFDGCAGLRRLF"
     gene            complement(2159..5452)
                     /locus_tag="VEL74_02325"
     CDS             complement(2159..5452)
                     /locus_tag="VEL74_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61393.1"
                     /translation="MTEAIHQMFQRTAERRGDHVAIETPGRRVTYRELRERANGIAAT
                     LLAGGAKRGVLVGLLASRTEDLAAGMLGVLQAGCAFMPLDRHSPAVRLESVVAEVRPR
                     WWLVSPDLAGLAGDLRRDVSDAGAIILLGDEGPRSEQAPAVPEPDPESLCYVFFTSGS
                     TGRPKGIAGRLKAIDHFIRWEIETFAIGDDCRVSQLTSPAFDAVLRDLFVPLAAGGTL
                     CAPADREVVLDGARLADWIHGERLTLVHYTPTVFRSLLRQELTADRFPALRQVLLAGE
                     PLLPADVRRWRAVFGDRIELVNLYGPSETTMIKLFHRVRPEDGEARMIPVGQPLPGAR
                     VIVLDEEEAPCPPGQVGEIVLRTPYRSLGYLNQPELTASVFVRNPFSDRPDDLIYRTG
                     DLGRLREDGLLELLGRRDQQVKVRGVRVEIAPIEEALRSHPAVRDAAVVDRTDTQGDK
                     FLCAYLVMGAGPGLSEEPAELDAGRLRDFLAARLPDAMVPSAFVRLESLPRTLTGKLD
                     RRALPDPDRLAARLGAERLAPRTPLEEKLGALFQELLGVPEVGVRESFFALGGHSLLA
                     AQLLARIRAELGVEVPLGRIFEAATVESLAGEVARLQAGHPAVTGIPLLPEADSYPLS
                     FAQQRLWLVDQVETESSFYNIAAAVRLLGRLRVPLLRQALDEVVRRHESLRTVFAADR
                     GRPRQIVRPPAPVALPVADLSGLPEGARLEEARRRAAAVAAGAFDLARGPLLRCLLLR
                     LAADEHVMAYAVHHITADQWSTRLLAQEARAAYLALAGGSPPALPELPIQYRDFAGWQ
                     RERLDDAALAERLAWWRGRLAGAPVFLRLPTDHPRPAVQSFRGAREVWDLPAALAEPL
                     RVLGIAEGGGLFPLFLTAFQILLGTRARQEDFVIASPVGFRDRHETENLIGLFVNTLL
                     LRADLTGDPSLRALLARVRRHLLEAYAQGDVPLDRVIEELAPDRTLSFSPFLQAGLNF
                     VELPPDEEGAAELTARPFGFDDGLSQFELNLILIHSAGSFQACLQYRHDLFARGTVAA
                     MARELEVILEWLALRPDAALSAVQEALAREEESRRSREKARAESAARERFRLRARKEP
                     DAAEAREGA"
     gene            complement(5466..7337)
                     /gene="asnB"
                     /locus_tag="VEL74_02330"
     CDS             complement(5466..7337)
                     /gene="asnB"
                     /locus_tag="VEL74_02330"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HYG61394.1"
                     /translation="MCGIAGCFDLQGRGRVERETLAAMTAALAHRGPDADGFHVEPDV
                     GLGFRRLRIVDLVTGDQPLYNEDGSLALICNGEIFNEPELRRELLGKGHVFRSRSDVE
                     VLLHLYEEHGVGLLDRLNGQFAFALYDARRRRLLLARDHFGVNPLYFTTTDGLFLFAS
                     EVKALLRHPAAPRGIDLIGLDQILSLPGAVSPRSLFVGIESLKSGHYLLAEDGKVTLR
                     EYWDLDYPEGEEGGDSGMTEEQYLEAMAEALRTSVRRRLRADVPVGVFLSGGLDSSLI
                     AALAMEAVPEAAGRSFSITFRDAEIDESHFQRLMARRLGTRHHEILVDEAMIVARLRD
                     MVYHCECPVKETYNTCALALAESVRAAGVTVVLAGEGSDELFGGYPGYRFDAFGRGDR
                     PVDELEEALEEELRERVWGDRRIFYEKDQVAFRDVKKALYAPAVRERFLQVDCMRQPL
                     VDRARLRNRHPLHQRSYLDFKLRLADHLLSEHGDRMVMAHSVEARYPFLDIELVELAR
                     CMPPGLKVKGFVEKYPVKWMAEKVLPRSIVEREKFGFRAPVSSRLLQNGCAWAHDLLS
                     PDLIRRQGVFDAGLVESLRRSYSEPGFRLHPHLDTDLLLVVLTFNLLCEVFALPALA"
     gene            complement(7340..12988)
                     /locus_tag="VEL74_02335"
     CDS             complement(7340..12988)
                     /locus_tag="VEL74_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG61395.1"
                     /translation="MCVPDAMTPHEEHIASVVAEVLGRPQVGREDDLFALAGASLPER
                     RQLAGRLAARLREALGREVPLAALIAHPSVARLAAWIGASQAGAAVPPIERAASRQEV
                     PLSYGQQRLWFLAQLEPGAATYNVPLVLEWGGALDLAALEASLHAIEQRHEILRTKFR
                     QRAGQPYQAVAGSAAAALPVVDLSRLPRARREGVAAWCAQVESQRPFDIENGPVWRFR
                     LLRLDGGLHWLVLTLHHIVCDAWSIDVLLYELGACYRAFAAGGTGPALPTLPIQYGDY
                     ALWQHRLLSREALAAPLTAWKRRLAGHTPVLELAVDRTPPALRTDAGASIAVELPARL
                     LDAAKEQGRRQGATLFMTVLAVFEALLARYTGRRRFLVGTPVANRTRPELKGMIGYFA
                     NTLVLPATVAPEAPFRELLAQAREVAVAALRHQDLPLEMLVQELAAEREQPRLPLLQA
                     ALTVHNEPTAVPAMPGSSLRLLEVGGSGARFELSLVGLETQDGLGLLLEYSTELFSRP
                     AAQRLLGHFAVLFEAAVTSPEQRLSELPLISPGERQQLLREWNDTRAAFPGTTLAHQF
                     FEAAAERTPEAVAAVCAGSELTYAELDAASNRLAHLLRRRGIRRGAPVGVWVERSLDL
                     LTAVLGVLKAGAHYVALDDTWPAGRVEAILASTGAPALVAGPGLLGAVEEMRWRLPAL
                     ADVVCLGIAEPEPPAEALDPESVRELWDLVAERAVDRATAGGFISAFTDQPMSEAEVD
                     EYRYRVLALAGPWLHAGARVLEIGNGSGLLLWEMATRVEHVTGVDPSPLTQERNRERA
                     EREGVANVRLATSFAHELDRVLGDGERFDLILLASTVQFFPGLRYLERVIRQAAGRLA
                     PGGALLIADVLDARRREELRRAVEERRGGAGAARRQELYLDEGLFRDLGATIHHRTEG
                     FANELGFRYDVLLRRDDLVAPEERGRRLWTRWHADRCPAGRLPQVAGLEDIAYVIHTS
                     GSTGEPKGIVVQHRPVANLIDWINRTFEVGPEDRGLFVTSLCFDLSVYDIFGMLAAGG
                     TVHVATREELGDPSHLVRLLRSGGITLWDSAPAALVQLAPLFPVAPDTDSRLRRVLLS
                     GDWIPVTLPDRVRQAFPRARVMALGGATEATVWSNWFPVGEVDPSWPSIPYGRPMANA
                     RYHVLDRGLFPCPVGVPGDLYIGGDCLCAGYRQPELTAGAFLPDPFSDAAGARLYRTG
                     DRARTQADGIIEFLGRLDQQVKVRGYRIELGEIEVTLVRHPGVREAVVLVREDEPGDR
                     RLVAYLVPASEPAPEPAALREALRGSLPEYMLPAAFVTLAELPVTPNGKLDRAALPAP
                     RWSAGTVGDVGAAGTGAPRTPMEETLGAIWASVLGVAAVGREDSFFDLGGHSLLATQM
                     LSKVREACAVEISLRTVFDRPKLAELAAEIEAMRSAGTAPARQPAPGRAGGSGPLPLS
                     FAQQRFWFLQQLNSDSSAFHLSGAVRLSGHLDAAVLAASFGEIVRRHAAVRTRFVAVA
                     GVPCQVVDPPAPFLLPRVDLSGLGGGARERELRRVAQSEAHRPFRLAAEPPLRVLLLR
                     LADGEHVVLFSLHHIAGDGWSLEILTRELGDLYAAGVDGRPSPLPELPVQYADWAVAH
                     QEWLRGPDAAEQLAWWRRQLGGDLPVLALPMQRQRPAAPRFRGATHHLALGEDLSVGL
                     ADLGRSAGATLFMSLLAAFKALLHLVTGGEDLLVGTNVANREWPGTEGLIGLFVNDLA
                     LRTDLAGRPSFRELLGRVRETVLAAHAHQDLPFEAIVADLRPERSAGPLFQVMLVLQT
                     FGLPVRELPGLTLATLAGEPRTANFDLVLTLSQGPAGLAGAFVYDVDLFEAPAIARLA
                     EHFTALLHAVVAEPDRPLHNLSFAHRDAARQMASAFSEDL"
     gene            complement(12967..15573)
                     /locus_tag="VEL74_02340"
     CDS             complement(12967..15573)
                     /locus_tag="VEL74_02340"
                     /inference="COORDINATES: protein motif:HMM:NF016615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HYG61396.1"
                     /translation="MTVALPDLTAYPAPAEGDTLHEHLIALPGGTWTLWRMVVLRGAG
                     FPALQVLRLAAPASADAADSVLRHEETLRVARAGALHAVHDALDGLRRDGLWADKELR
                     KPLMKAMQALTGGKVPKDDPAPSCAPAFETLRTAWAGLEAARAEMARIYPAEVARVSE
                     EIADIAREDRFREAVLWQNRHAVETALDELAARPAAARARTSKRRQHEELAASYLQRY
                     CTKNDTIGFFGPVAFSRLNDEGEAVSVRCGAGLVQQRAVYFETWCIEALAETLGRNPA
                     LRPWLAPRLKSSFHLHDRTLHRPFGKPIALPEAQASLIARCDGRRVAYEVARDLVADP
                     AAPFRTEEEVYRLLEELCKGRVLTWALQVPPDLHPDRRLAELLSRIGPEPLRAEALAT
                     VEAMQRARDRVASAAGDPNALDAALRDMESTFRRLTGTTPRQREGQTYAARGLVYEDC
                     RRDIEVAFGPELTRRMGPPLSLVLRSARWLAAELARGAEEKLRALYAQLRRHTGTDAV
                     DAYAFFTSALPAIFFHPGRKETLAAIERDLYARWSRVLGPLPEGERRVRFAVSELEDR
                     FAAEFGDAGPVWTLIHYFSPDVMISAEGEEAFRRGNLELVLGEVHGGNTLLWSCFISQ
                     HPASEEVTRFLEREAGSATVVLPQALQHGWPRRVSHGLTLPGGHYFQFADDLPSGPEG
                     LPLPAGDLVIEETAGGVRARTRDGRVSFHPIDLFAAYLTNECTALIGGILEPAPHTPR
                     VSFDDMIIARERWSFTAAELDFAEIQDPQDRFLALRRWAGSLGLPRFCFFKVEIERKP
                     CYLDLDSPISGDLFARFVRAAREAGPEVRVSVSEMAPRLDQAWLRDAAENLYTCELRL
                     AASDRECVFLTP"
     gene            complement(15570..>16168)
                     /locus_tag="VEL74_02345"
     CDS             complement(15570..>16168)
                     /locus_tag="VEL74_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYG61397.1"
                     /translation="VRAAAVLAFPTETGELGELRLVAYVAAVGERVPEGSNLRSHLER
                     RLPAYMVPSAFKVRAELPCTSSGKIDRSALAGLGDPFAEREGGAMPETPLDKVLAGMI
                     AEVLGLQGGVGLHDNFFELGGHSMRAVELVARLRDAFGIDVPLFHVFDAPTVKELAAV
                     LMGAPEWGPIVEELAPALLQLIEPPAEPMGASGQEEKR"
BASE COUNT         2407 a         5886 c         5906 g         1969 t
ORIGIN      
        1 cagaagagcc gccggagccc ggcacagccg tcgaagatcc cttcctgctc caggaacacc
       61 gagagctggg acggcacgag ttggagcacc gtcacgccgc ggcgggcgat cgtctccagg
      121 aggtaggagt tgtcgcgctg ccctcccggc tcggccagca acagacgggc gccggcgatc
      181 agagggacga agatctccca gaccgacgca tcgaagctgt atggggtctt ctgcaggacg
      241 cggtcgtccg cccccagggg gaagcgccgc tgcatccaga gcaggcggtt gaggatcgcc
      301 cggtgggaga tcatcacccc cttcggccgc ccggtcgagc cagaggtgta gatcacatag
      361 gccagatcgt cgggggcggc gccgggcccc tgccactccc cggcctccag ctcgaggcgg
      421 tccaggcaga cgacccggaa ccgcccctcc gggaagcggc cggcgagtgc ctggcgggtg
      481 aggaggacgg ccgccccggc gtcctccagc atcctgtcga ggcgctcctg gggataatcg
      541 gggtcgagag gcacgtaggc ccctccggct gccagcactc ccaggaggct cactaccatc
      601 tccggcgagc gctcgaccgc cacggcgacc ctcacttcgg ggccgatgcc cagccggcgc
      661 aggtgggagg ccagccggga ggcacggtcg atgagctcgg cgcgcgtcat ctcaccgccg
      721 tcccactcca gggcggtgcc gtgcgggtcg cgcgccgccc gctcggcaac ccgctcccac
      781 agggttcccg ccggcccgga atcggtgccg gtggcgttcc actccaccag cgtccgctgg
      841 cgctcggcgc cgccgaggat ctccaggtcg ccgacgcggc tctcgggagc cgccagggcg
      901 ttccggagca gcgtctcgta ccagcgcccc agccgctcca cgtcggcgtg acccaggagg
      961 ccggcgtcgt agacgagatc ggcgccgagc gaggtgacct cacggcggta ccgctggacg
     1021 cggagcatca cctcgaaacg ctcgctccag cagtgacgcc gggccatcgt gaaggtcagg
     1081 ccgcccgcct cggccggcgg ccggacatcg gtgagctcga acgcgaaggg gagcgccgcg
     1141 gctcgtgcgc caccggcggc gaggtcccag tcgaagtact cctgccaatc gtcgagctct
     1201 cgtagcgacg cgcccacccg ccgtaccagg gaggtgaagg ggccctcccc gggcgcgcac
     1261 gccagcggca ggcagcgggc atagggaccg agcgccgcca ccatctcctc gtacttgcgg
     1321 ccgccgcgca ccacgccgag gacgagctcc tcccggtccg ccaccagacc cagcaggagc
     1381 tgccagaccg cgagcagcac cgcctccagg cccacctccg gcggcgcccc gctctgctcc
     1441 gccaccgccg acgcgtgcgc cgcgaggtcg ccgggaaaag agagcgccac cgtgcgcggc
     1501 gtgaagcctg ccggcagctc cgctcccggg ctccggagcg agaacggcag cgcggcctcc
     1561 agcccgtcgg ccggagcggc gcggcgccag aagctcttcc cttcggcaga ctcctccagc
     1621 gtcaggatct cgttgttcca ggcggcgagg tcggcgtatt ggaaggggtc cgtcatctcg
     1681 ccgcggccat aggcgcgggc gatctccagc gtcaggttgt ccaggctggc gccgtcgccg
     1741 cagagagcgg gcaggccgag cagcagcagg tggcggccgg aaccgaggca ggcgacggcg
     1801 gcgcgcagcg gctctgggcc gtccgcatcg tcagcggcgg cggtttgcca ttccgcgagg
     1861 agctggtcta gcggcgtgcc ggcccctgat gacaggctcg cgagccgcgg cagggcatcg
     1921 ccgatcacct gcagcggata aggcatcccc ggtaggacgt ggaacgacgt gcgcaggatc
     1981 tcgtggcggc gcgccacgac ttccagcgcc acttggaggg cgcccggatc gaggtcgccg
     2041 gcgatctcta ccacgcaggc gctccggtag gggtggaacg acgggctcgg gcagagttgc
     2101 cagagacggc gctgctgggg cgagaggggc agcccctgca gggaggtggc ttcctggatc
     2161 aagcgccctc cctggcttcg gccgcgtcgg gctccttgcg ggcgcgcaga cggaagcgct
     2221 ccctggccgc gctctccgcc ctggccttct ctcgcgaccg ccggctctcc tcctcgcggg
     2281 cgagggcctc ctgcacggcg gagagcgccg cgtccggccg cagggcgagc cactccagaa
     2341 tcacctcgag ctcgcgcgcc atggcggcga cggtaccgcg cgcgaagaga tcgtggcggt
     2401 actgcaggca ggcctggaac gagccggcgg agtggatcag gatgaggttc agctcgaact
     2461 gcgacagccc gtcatcgaac ccgaaaggcc gggcggtcaa ctccgccgcc ccctcctcgt
     2521 ccggtggcag ctccacgaag ttgagacccg cctgcaggaa ggggctgaag ctcagcgtcc
     2581 ggtcgggagc cagctcttcg atgacccggt cgagaggaac gtcgccctgc gcgtaggctt
     2641 cgaggaggtg acggcgaacc ctggcgagga gggcgcgcag ggacggatcg ccggtgagat
     2701 cggcgcgcag cagcagggtg ttgacgaaca gaccgatcag gttctcggtc tcgtggcggt
     2761 cgcggaatcc cactggcgag gcgatgacga agtcctcctg ccgggcacgg gtgccgagga
     2821 ggatctggaa cgcggtcagg aagaggggga agagcccgcc cccctcggcg atgccgagca
     2881 cgcgcagggg ctcggcgagc gcggccggca ggtcccatac ctcacgggcg ccgcggaagc
     2941 tctgcaccgc cggccgcggg tggtccgtgg gcagccgcag gaagaccggc gcgccggcga
     3001 gccgcccgcg ccaccaggcg agccgttccg ccagcgccgc gtcgtcgagc cgctcgcgct
     3061 gccagccggc gaaatcccga tactggatcg gcagctccgg caacgccggc ggactccccc
     3121 ccgccagggc gagataggca gcgcgcgcct cctgtgccag caggcgggtc gaccactgat
     3181 cggcggtgat gtgatgcacc gcatacgcca tcacgtgctc gtcggcggcc aggcgcagga
     3241 gcaggcagcg cagcaggggg ccgcgcgcca gatcgaaggc gccggcagcc accgccgcgg
     3301 cccgccgccg cgcctcctcc agccgcgcgc cctccggcag gccgctcagg tcggccaccg
     3361 gcagcgccac cggagccggc ggccggacga tctgccgggg gcgcccacgg tcggcggcga
     3421 agacggtgcg cagcgactcg tggcgcctca ccacctcgtc cagggcctgg cgcagcagcg
     3481 gcacgcgcag ccggccgagc aggcgcaccg ccgcggcgat gttgtagaac gagctctccg
     3541 tctccacctg gtcgaccaac cagagccgct gctgggcgaa cgacagcgga tacgagtccg
     3601 cctccggcag cagcggaatc ccggtcacgg cggggtgacc ggcctgcaac cgggccacct
     3661 cgccggcgag cgactcgacg gtcgccgcct cgaagatccg tcccagcggc acctcgacgc
     3721 ccagctccgc ccggatgcgg gcaaggagct gtgccgccag gagcgagtga ccgccgagcg
     3781 cgaagaagct ctcgcggacc cccacctccg gcacgcccag cagctcctgg aagagagcgc
     3841 ccagcttctc ctcgagcggc gtccgcggcg ccagccgctc cgcgccgagc cgcgccgcca
     3901 gccggtccgg gtcgggcagg gcgcggcggt cgagcttccc cgtcagcgtg cgcggcaggg
     3961 actccaggcg cacgaaggcg gacggcacca tggcatcggg cagccgcgcc gccaggaaat
     4021 cccgcagccg gccggcgtcc agctccgccg gctcttccga aaggccggga ccggcgccca
     4081 taacaaggta ggcgcagagg aacttgtcac cctgggtgtc cgtgcggtcg accaccgccg
     4141 cgtcgcggac cgcagggtgg gagcgcagcg cctcctcgat gggcgcgatc tcgacgcgga
     4201 cgccgcgcac cttcacctgc tggtcgcgcc ggccgaggag ctcgaggaga ccgtcctcgc
     4261 gcaggcggcc gaggtcgccg gtgcggtaga tcaggtcgtc gggacggtcg ctgaaagggt
     4321 tgcgcacgaa gacggacgcc gtcagctccg gctggttcag gtagcccagc gaccggtacg
     4381 gtgtgcggag aacgatctcc cccacctgcc cgggagggca gggggcctcc tcctcgtcaa
     4441 gaacgatcac ccgcgccccc ggcagcggct ggcccacggg gatcatccgt gcctccccgt
     4501 cctccggccg cacccggtgg aagagcttga tcatggtggt ttcgctgggc ccgtagaggt
     4561 tgaccagctc gatgcggtcg ccgaagacgg cgcgccatcg ccggacgtcc gccggcagca
     4621 gcggctcgcc ggcgaggagg acctgccgca acgccgggaa gcggtcggcc gtcagctcct
     4681 gccgcaacag cgaacggaag accgtcggtg tgtagtggac cagggtcagc cgctcgccgt
     4741 ggatccagtc cgccagccgc gcgccgtcga ggacgacctc acggtccgcc ggggcgcaca
     4801 gcgtgccgcc ggcggccaga gggacgaaga gatcgcgcag gacggcgtcg aaggccggcg
     4861 aggtgagctg gctgacgcgg cagtcgtcgc caatggcgaa ggtctcgatc tcccagcgga
     4921 tgaagtgatc gatcgccttg agccggccgg cgatcccctt tgggcggccg gtggagcccg
     4981 aggtgaagaa gacgtagcag agcgactccg gatcgggctc cggcacggcg ggggcctgct
     5041 cgctgcgcgg cccctcgtcg ccgagcagga tgatcgcacc ggcgtcgctg acatcgcgcc
     5101 ggagatcgcc ggccaggccg gcgaggtccg ggctcaccag ccaccagcgg ggcctcacct
     5161 cggcgacgac gctctccaga cggacggccg gagagtggcg atcgagaggc atgaaggcgc
     5221 accccgcctg gaggacgccg agcatgccgg cggcgagatc ttccgtgcgg ctcgccagca
     5281 ggccgaccag cacgccccgc ttcgccccac cggccagtag ggtcgcggcg atgccgttcg
     5341 cccgctcccg cagctcgcga taggtgaccc gccgcccggg cgtctcgatg gcgacgtggt
     5401 cgccgcggcg ctcggcggtc cgctggaaca tctggtgaat ggcttcggtc atcacgtctc
     5461 cgggctcagg ccagggccgg cagggcgaag acctcgcaca gcaggttgaa cgtcagcacc
     5521 acgagcagca ggtcggtatc gaggtgcgga tgcaggcgga accccggctc cgaatagctc
     5581 cggcgcagcg attccaccaa gccggcatcg aagaccccct ggcggcggat gaggtcgggc
     5641 gacaggaggt cgtgggccca ggcgcagccg ttctgcagca gccgagagct caccggcgcg
     5701 cggaagccga acttttcccg ctcgacgatc gaacgcggca gaaccttttc ggccatccac
     5761 ttcaccggat acttctcgac gaagcccttg accttcaaac ccggcggcat gcagcgcgcc
     5821 agctccacca gctcgatgtc caggaacggg tagcgcgcct ccaccgagtg cgccatcacc
     5881 atgcggtcgc cgtgctcgga gaggaggtgg tcggccaggc gcagcttgaa gtcgaggtag
     5941 gaacgctggt gcagcgggtg gcggttgcgc agccgcgcgc gatccaccag cggctgccgc
     6001 atgcagtcga cctgcaggaa ccgctcgcgc accgcgggcg cgtagagggc cttcttgacg
     6061 tcgcggaagg cgacctggtc cttctcgtag aagatccgcc ggtcgcccca gacgcgctcg
     6121 cgcagctcct cctccagggc ctcctcgagc tcgtccaccg gccggtcgcc gcggccgaag
     6181 gcgtcgaagc ggtacccggg atagccgccg aacagctcgt ccgatccctc gccggcgagc
     6241 acgacggtga cgccggcggc gcgcaccgat tcggccagcg ccagggcgca ggtgttgtag
     6301 gtctccttca ccgggcactc gcagtggtag accatgtcgc gcaggcgggc gacgatcatc
     6361 gcctcgtcga ccaggatctc gtggtggcgg gtgccgagcc ggcgggccat caggcgctgg
     6421 aagtgggact cgtcgatctc cgcgtcgcgg aaggtgatgg agaacgaacg gccggcggcc
     6481 tcggggaccg cctccatggc cagcgcggcg atgagcgacg aatcgaggcc gccgctgagg
     6541 aagacgccca ccggcacgtc ggcccgcagg cgccgccgca cgctggtgcg cagggcctcg
     6601 gccattgcct cgaggtactg ctcctccgtc atgccggagt cgccgccctc ctcgccctcc
     6661 gggtagtcga ggtcccaata ctcccggagc gtcacttttc catcctcggc cagcaggtag
     6721 tggccgctct tcaggctctc gatccccacg aacagggagc gcgggctcac cgcgccgggc
     6781 agggatagga tctggtcgag cccgatcaga tcgatgccgc gcggcgccgc cggatgccgc
     6841 agcagcgcct tgacctccga ggcgaagagg aacaggccgt cggtggtcgt gaagtacagc
     6901 gggttgacgc cgaaatggtc gcgcgccagg aggagccggc gccggcgtgc gtcgtagagg
     6961 gcgaaggcga actggccgtt cagccggtcc agcaggccca ccccgtgctc ctcgtaaaga
     7021 tggaggagga cctccacgtc gctgcgcgaa cggaagacgt gacctttccc cagcagctcg
     7081 cgccgcagct cgggctcgtt gaagatctcg ccgttgcaga tcagcgccag ggagccgtcc
     7141 tcgttgtaga gcggctggtc gcccgtcacc aggtcgacga tgcgcaggcg gcggaagccc
     7201 agcccgacgt ccggctccac gtggaagccg tccgcgtccg gtccccggtg ggcgagcgcc
     7261 gcggtcatgg cagccagcgt ttcgcgctcg acgcggccac ggccctggag atcgaagcaa
     7321 ccggcgatcc cgcacatcgt cagaggtcct cgctgaaggc gctggccatc tgccgcgcgg
     7381 cgtcccggtg ggcgaacgac aggttgtgga gcggacggtc gggctcggcc accacggcgt
     7441 ggaggagggc ggtgaaatgc tccgccaagc gggcgatggc cggggcctcg aacaggtcga
     7501 cgtcgtagac gaacgccccg gcgaggccag ccggcccctg gctcagggtc aggaccaggt
     7561 cgaagttggc cgtccgcggc tcgcccgcga gcgtcgcaag cgtgagcccc ggcagctccc
     7621 gcaccggcag gccgaaggtc tgcagcacga gcatcacctg gaagagcggg cccgccgaac
     7681 gctcggggcg caagtccgcg acgatggctt cgaacggcag gtcctgatgg gcatgggccg
     7741 ccagcacggt ctcccgcacc cggcccagca gctcgcggaa gctcggccgg cccgccagat
     7801 cggtgcgcag cgcgaggtcg ttgacgaaca ggccgatgag cccctcggta ccgggccatt
     7861 cgcggttggc gacgttggtg ccgacgagca ggtcctcgcc gcccgtgacc agatgcagca
     7921 gggccttgaa cgccgcgagc aggctcatga acagggtcgc tcctgcgctc cggccgaggt
     7981 ctgccaggcc gaccgagagg tcctccccga gcgccaggtg atgcgtggcg ccgcggaaac
     8041 gtggcgccgc ggggcgctgc cgctgcatgg gcagggcgag gaccggcagg tcgccgccaa
     8101 gctgccggcg ccaccaggcc agctgctccg cggcgtccgg accgcgcagc cactcctgat
     8161 gggccacggc ccagtcggcg tactggaccg gtagctcggg gagcggcgaa ggccggccgt
     8221 ccacgccggc ggcgtagagg tcgcccagct cccgggtgag gatctcgagc gaccagccat
     8281 cgccggcgat gtgatgcagc gagaacagga ccacgtgctc gccgtcggcc aggcgcagca
     8341 gcaggacgcg cagcggcggt tccgcggcga ggcggaacgg gcggtgcgcc tcgctctgcg
     8401 cgacgcgccg cagctcgcgc tcccgcgcgc cgccgccgag gcccgagagg tccacgcggg
     8461 gcagcaggaa cggcgccggc ggatcgacca cctggcaggg caccccggcc accgccacga
     8521 agcgggtgcg gaccgcggca tggcgccgca cgatctcgcc gaagctcgcc gccagcacgg
     8581 cggcgtcgag atggccggag aggcggacgg cgccactcag gtggaaggcg gacgaatccg
     8641 agttgagctg ctgcaggaac cagaagcgct gctgggcgaa ggagaggggc agcggcccgc
     8701 tcccgccggc ccgcccgggg gccggctgcc gcgccggcgc cgtgcccgcc gagcgcatgg
     8761 cctcgatctc ggccgccagc tccgccagct tcggccggtc gaaaacggtg cgaagggaga
     8821 tctccacggc gcaggcctcg cgcaccttgg agagcatctg cgtcgccagc agagagtgcc
     8881 cgccgaggtc gaagaagctg tcctcccgcc cgaccgcggc cacgccgagg accgaggccc
     8941 agatcgcccc cagggtctcc tccatgggag tccgcggcgc ccccgtccca gcggctccga
     9001 cgtcgccgac ggtgccggcg ctccaccgcg gcgcgggcag cgcagcgcgg tccagcttgc
     9061 cgttgggcgt caccggcagc tccgccaggg tgacgaacgc cgcggggagc atgtactcgg
     9121 ggagtgatcc tcgcagggcc tcgcgcagcg ccgccggctc cggcgccggc tcgctcgccg
     9181 ggaccaggta ggcgaccagg cgccggtccc ccggctcgtc ctcccgcacc agcaccactg
     9241 cctcgcgtac cccaggatgg cgtaccagcg tcacctcgat ctcgccgagc tcgatgcggt
     9301 agccccgcac cttgacctgc tggtcgaggc gccccaggaa ctcgatgatg ccgtcggcct
     9361 gtgtgcgggc gcggtcaccg gtccggtaga gacgggcccc ggcagcgtcc gagaagggat
     9421 cgggcaggaa ggccccggcg gtcagctccg gctggcggta gccggcacag aggcagtcgc
     9481 cgccgatgta gaggtccccg ggcacgccca ccggacaggg gaagaggccg cggtccagga
     9541 cgtgatagcg cgcgttcgcc atcggccggc cgtaggggat gctcggccag gacggatcga
     9601 cctcccccac cgggaaccag ttggaccaca ccgtggcctc ggtggcgccg cccaaagcca
     9661 tcacccgcgc ccgcgggaag gcttggcgca cgcggtccgg cagggtcacc gggatccaat
     9721 cgcccgacag cagcacccgc cgcagacggc tgtcggtgtc cggcgccacg gggaagaggg
     9781 gcgcgagctg gaccagggcg gcgggcgccg aatcccacaa cgtgatgccg ccgctccgca
     9841 ggaggcgcac caggtggctg gggtcgccga gctcctcccg cgtcgccacg tgcaccgtgc
     9901 cgcccgcggc gagcatgccg aagatgtcgt agaccgagag gtcgaagcag agcgaggtga
     9961 cgaacagccc acggtcctcc gggcccacct cgaaggtgcg attgatccag tcgatcaggt
    10021 tggccaccgg ccggtgctga acgacgatgc ccttgggctc gccggtcgag cccgaggtgt
    10081 ggatgacgta ggcgatgtcc tccagaccgg ccacttgggg caggcggccg gcggggcatc
    10141 ggtcggcatg ccagcgggtc cacaggcgcc ggcccctctc ctccggcgcc accagatcgt
    10201 cccgcctcag gagcacgtcg tagcggaagc ccagctcgtt ggcgaagccc tcggtccggt
    10261 ggtggatggt ggcgcccaag tcgcggaaga gcccctcgtc gaggtacagc tcctggcgcc
    10321 gggcggcgcc ggcgccgccc cggcgctcct cgaccgcccg ccgcagctcc tcgcgccgac
    10381 gggcgtcgag cacgtcagcg atgagcagcg cgccgcccgg cgcgagccgc ccggccgcct
    10441 ggcggatcac ccgctccagg tagcgcaggc cggggaagaa ctggacggtg ctggcgagga
    10501 ggatcaggtc gaaccgctcg ccatcgccga gcacccggtc cagctcgtgg gcgaagctgg
    10561 tggcgagccg cacgttcgcc accccctccc tctccgcccg ctcgcggttg cgctcctggg
    10621 tcagcggtga gggatcgacg ccggtgacgt gctccacgcg ggtggccatc tcccagagca
    10681 ggagaccgga gccgttgccg atctccagca cccgcgcccc ggcgtgcagc cacgggccgg
    10741 ccagggcgag gacccggtag cggtactcgt cgacctcggc ctcgctcatc ggctgatccg
    10801 tgaaggcgct gatgaagccg cccgcggtcg cccggtccac cgcccgttcc gccaccaggt
    10861 cccacagctc gcgcacgctc tccgggtcga gcgcctcggc cggcggctcc ggctcggcga
    10921 tccccaggca gaccacgtcg gcgagcgccg gcagccgcca gcgcatctcc tccaccgcgc
    10981 cgagcagccc ggggccggcg acgagagcgg gcgcgccggt ggaggcgagg atcgcttcga
    11041 cccgcccggc cggccaagtg tcgtccaggg ccacgtagtg cgcgcccgcc ttgagcacgc
    11101 cgaggaccgc ggtgagcagg tccagcgagc gctcgaccca gacccccacc ggcgcgccgc
    11161 gccggatgcc tcggcgccgc agcagatggg ccaggcggtt gctcgccgcg tcgagctcgg
    11221 cgtaggtcag ctcgctcccc gcgcagacgg ccgccaccgc ctcgggtgtc cgctccgcgg
    11281 cggcctcgaa gaactggtgc gcgagggtcg tcccggggaa ggcggcgcgc gtgtcgttcc
    11341 attcgcggag gagctgctgc cgctcgccgg gcgagatgag cggcagctcc gacaggcgct
    11401 gctccgggct ggtcaccgcg gcttcgaaaa ggaccgcgaa gtgccccagc agccgctggg
    11461 cggcggggcg ggagaacagc tccgtgctgt actcgagcag caggccgagg ccgtcctgcg
    11521 tctccagccc caccaggctc agctcgaacc gcgccccgct gccccccacc tccagcaggc
    11581 gcaggctcga gcccggcatg gccggcaccg cggtcggctc gttatgtacc gtgagcgccg
    11641 cctggagcag gggcaggcgg ggctgctcgc gctccgccgc cagctcctgg acgagcatct
    11701 ccagcggcag gtcctgatgg cggagcgcgg cgaccgccac ctcgcgcgct tgcgccagca
    11761 gctcgcggaa gggggcctcg ggagcgaccg tcgcgggcag gaccagggtg ttggcgaagt
    11821 agccgatcat ccccttgagc tccggccgcg tccggttggc gaccggcgtc cccaccagga
    11881 accggcggcg gccggtgtaa cgcgccagga gagcctcgaa gaccgcgagc acggtcatga
    11941 atagcgtggc accctggcgc cggccctgct ccttggccgc gtccagcagc cgcgccggga
    12001 gctcgacggc gatggaggct ccggcgtcgg tccgcagggc cggcggggtg cggtccaccg
    12061 ccagctccag caccggcgta tggcccgcca gccgccgctt ccaggcggtg agcggggcgg
    12121 ccagcgcctc gcggctgaga aggcgatgct gccagagcgc gtagtcgccg tactggatgg
    12181 gcaaggtggg cagcgccggc cccgtgcccc cggcggcgaa ggcgcgatag caggcaccga
    12241 gctcgtacag cagcacgtcg atcgaccagg cgtcacagac gatgtggtgg agggtcagca
    12301 ccagccagtg cagaccgcca tccaggcgca gcagccggaa ccgccacacc ggtccgttct
    12361 cgatgtcgaa ggggcgctgg ctctccactt gcgcgcacca cgccgccacc ccctcgcgcc
    12421 gagcgcgcgg cagccgcgag agatcgacca ccggcagcgc cgcggcggcg ctccctgcca
    12481 ccgcctggta gggctgcccg gcgcgctgcc ggaacttcgt ccgcaggatc tcgtgccgct
    12541 gctcgatggc gtggaggctc gcctccagcg ccgcgaggtc gagagccccc ccccactcca
    12601 gcaccagcgg cacgttgtag gtcgccgcgc cgggctcgag ctgggccagg aaccagaggc
    12661 gctgctggcc gtaggagagc ggcacctcct ggcgcgacgc ggcccgctcg atgggcggca
    12721 ccgccgcgcc ggcctgcgag gcgccgatcc aggccgccag gcgggccacc gacggatggg
    12781 cgatgagcgc cgccagcggc acctcgcgcc ccagcgcctc gcgcagccgc gccgccaggc
    12841 ggccggccag ctgccgccgc tccggcagcg acgcgccggc cagcgcgaag aggtcgtcct
    12901 cgcgcccgac ctgcggccgg ccgagcacct cggcgacgac gctggcgatg tgctcctcgt
    12961 gcggggtcat ggcgtcagga acacacactc ccggtccgag gcggccaggc gcagctcgca
    13021 ggtgtagagg ttctccgccg cgtcgcgcag ccatgcctgg tcgaggcggg gcgccatctc
    13081 ggagaccgag accctcacct ccgggccggc ctcgcgggcg gcgcgcacga aacgggcgaa
    13141 gagatcgccg ctgatggggc tgtcgagatc gaggtagcaa ggtttcctct cgatctccac
    13201 cttgaagaag cagaagcgcg gcaggccgag ggagcccgcc cagcggcgca gtgccaggaa
    13261 acggtcctgc ggatcctgga tctcggcgaa atccagctcc gcggcagtga aggaccagcg
    13321 ctcgcgggcg atgatcatgt cgtcgaagct cacccggggc gtgtggggcg ccggctccag
    13381 gatgccgccg atcagcgccg tgcactcatt ggtgagatag gcggcgaaga gatcgatggg
    13441 gtggaaggag acccggccgt cgcgggtgcg cgcccggact ccgcccgccg tctcctcgat
    13501 gaccagatcc ccggccggca gcggcaagcc ctcggggccg ctcggcagat cgtcggcgaa
    13561 ctggaagtag tggccgccgg ggagggtcag gccgtggctc acccgccgcg gccagccgtg
    13621 ctggagggcc tggggcagca ccacggtggc ggagcccgcc tcccgctcca ggaaccgggt
    13681 gacctcctcc gacgccggat gctgcgagat gaagcaggac cagaggaggg tgttcccgcc
    13741 gtgcacctcg cccagcacca gctcgagatt gccgcggcgg aaggcctcct ccccttcggc
    13801 ggagatcatc acatccgggc tgaagtagtg gatcagagtc cagaccggac cggcgtcccc
    13861 gaactcggcg gcgaagcggt cctccagctc gctgaccgcg aagcggacac gccgctcccc
    13921 ctccggcagc gggcccagca cccgggacca gcgggcgtag aggtccctct cgatggccgc
    13981 cagcgtctcc ttccggccgg ggtggaagaa gatcgccggc agggcgctgg tgaagaacgc
    14041 gtaggcatcg acggcgtcgg tgccggtgtg acggcggagc tgggcatata gcgcgcgcag
    14101 cttctcctcg gcgccacggg ccagctcggc cgcgagccag cgggcgctgc gcaggaccag
    14161 gctgagcggc gggcccatgc ggcgcgtcag ctcggggccg aaggcgacct cgatgtcgcg
    14221 ccggcagtcc tcgtagacca gcccgcgggc cgcgtacgtc tgcccctccc gctgccgggg
    14281 ggtggtgccg gtcagccgcc ggaaggtgct ctccatgtcg cgcagggccg cgtcgagggc
    14341 gttcgggtct cccgccgccg atgccacgcg gtcgcgggcg cgctgcatcg cctcaacggt
    14401 ggccagcgcc tcggcgcgca agggctcggg gccgatgcgg gagaggagct cggccagcct
    14461 gcggtccgga tgcaggtcgg gcggcacctg gagggcccag gtcagcaccc ggcccttgca
    14521 cagctcctcg agcagccggt agacctcctc ttccgtccgg aacggcgcgg cgggatcggc
    14581 gacgaggtcg cgcgccacct cgtaggcgac ccggcggccg tcgcagcggg cgatgaggct
    14641 cgcctgagct tccggcagcg cgatcggctt gccgaacggg cggtggaggg tgcggtcgtg
    14701 caggtggaag ctcgacttga ggcgcggcgc cagccagggg cggagcgccg ggttgcggcc
    14761 cagcgtctcg gccagcgcct cgatgcacca ggtctcgaaa tagaccgccc gctgctgcac
    14821 caggccggcg ccacagcgca ccgacaccgc ctctccctcg tcgttcagcc gggagaaggc
    14881 caccggcccg aagaagccga tggtgtcgtt cttggtgcag taccgttgca gatagctggc
    14941 ggccagctcc tcgtgctgcc gccgcttcga ggtccgcgcc cgggcggcgg ctggccgcgc
    15001 ggccagctcg tcgagggccg tctccacagc gtggcggttc tgccagagca cggcctcgcg
    15061 gaagcggtcc tcgcgcgcga tgtccgcgat ctcctcggag acacgggcga cctcggccgg
    15121 gtagatcctc gccatctccg cccgcgccgc ttcgagcccc gcccaggcgg tgcgcagggt
    15181 ctcgaaggcg ggagcgcagg agggggcggg gtcgtccttc ggcaccttgc cgccggtcag
    15241 cgcctgcatc gccttcatca gcggcttgcg gagctccttg tccgcccaca ggccgtcgcg
    15301 gcgcagcccg tccagcgcgt cgtggaccgc atggagggcg ccggcgcgag ccacccgcag
    15361 cgtctcctcg tggcgtagga cgctatcggc ggcgtcggcg ctggcgggcg cggccagccg
    15421 gagcacctgc agggcgggaa agccggcgcc gcgcagcacc accatgcgcc atagcgtcca
    15481 ggtgccgccg gggagcgcga tcaggtgctc gtgcagggtg tccccttccg ccggcgcggg
    15541 gtaggcggtg agatccggca gggccactgt cacctcttct cctcctggcc cgaggcaccc
    15601 atgggctcag cgggcggctc gatgagctgc agcaaagccg gcgcgagctc ctccacgatc
    15661 gggccccact ccggagctcc catgagcacg gccgccagct ccttcaccgt cggcgcgtcg
    15721 aagacgtgga agagagggac atcgatgccg aaggcatcgc gcaggcgtgc caccagctcg
    15781 acggcgcgca tggagtgccc ccccagctcg aagaagttgt cgtgcagccc caccccgccc
    15841 tggaggccga ggacctcggc gatcatcccg gcgagcacct tgtccagcgg ggtttcgggc
    15901 atcgctccgc cctcgcgctc agcgaacgga tcgcccaggc cggccagggc cgagcggtcg
    15961 atcttgccgc tgctcgtgca cggcagctcc gcgcgtacct tgaacgcgga gggcaccatg
    16021 taggcgggca gccgccgctc gagatgagag cgcaggttgc tcccctcggg aacccgctcg
    16081 ccgacggcgg ccacgtaggc caccagccgt agctccccga gctcgccggt ctcggtaggg
    16141 aacgccagca ccgcagccgc gcgcacct
//
LOCUS       DASPJI010000089         3143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_466532, whole genome shotgun sequence.
ACCESSION   DASPJI010000089 DASPJI010000000
VERSION     DASPJI010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466532"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VEL74_02350"
     CDS             <1..321
                     /locus_tag="VEL74_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HYG61398.1"
                     /translation="AGTGADLAHPAVLVAVVSVLGNLVGNVPAVMMLLPFVDREPVTG
                     YALALASTFAGNAVLVGSIANLIVAEQAEKLGIRFGFREHLRTGLPVTVVSLAVAAAA
                     MALW"
     gene            complement(793..1173)
                     /locus_tag="VEL74_02355"
     CDS             complement(793..1173)
                     /locus_tag="VEL74_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61399.1"
                     /translation="MSRQLEGAGGTPGGLGEFLVGFVMAVAGGYLLTTHVTVVSGHWT
                     LWGYNAFGLSLVPFIAGVALLFFNGKSKLGWLLLIAGVVIILAGIIANLNIYFEPTSL
                     FNTLIMLVLLFGGLGLMARGLRAH"
     gene            complement(1170..1694)
                     /locus_tag="VEL74_02360"
     CDS             complement(1170..1694)
                     /locus_tag="VEL74_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61400.1"
                     /translation="MNPVSQMHVAVIQYFSGEKRESVLFIAAGVLAIIASVVLLWKGS
                     PLRGMAYPLIAIALIQLVVGWTVFARTDGQTRSLHTHLQGDPGGYAAAELARMDKVQR
                     SFQIYKVIEIVLMAAGVAGLFLLRDRQTLYAVSRGLALQAGLMLAFDLAAERRADIYV
                     NAIRTLGRALEENR"
     gene            complement(1707..2306)
                     /locus_tag="VEL74_02365"
     CDS             complement(1707..2306)
                     /locus_tag="VEL74_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYG61401.1"
                     /translation="MPFNAWYRVRYTLYAPLYDRITKFHAERRRSVELLGLRPGERVI
                     IIGCGTGADIPFIPAFAEVLATDLTPAMLAQAKVHARPGVEFRRLDGMALELPDGSFD
                     AAILHMVLEVIPDPVRCLREAARVLRPGGRLAVFDKFLPDGVPISPLRRLGLALLDVV
                     FTSTNRRMGEILERSGAPFEVEHDERARGPYRHLLLRRR"
     gene            complement(2310..>3143)
                     /locus_tag="VEL74_02370"
     CDS             complement(2310..>3143)
                     /locus_tag="VEL74_02370"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HYG61402.1"
                     /translation="SSQALVDFAVEFRGKAAHAAYDPWNGRSALDAVEIFTHALNLMR
                     EHVRPTVRMHYVIAEGGDVPNVIPEKAKIWCWLRDTEHTSVDQLLVRVRDIVKGAALA
                     AGVEGELRVQSGDYEMLVNMAGARLIHSNLTWLGPLAFTEQEHEFARAIQKATNVEPK
                     GLDGAIQPLNENPGPPEGGSTDVADVSWNVPTLHLSVTTAPAGAPWHAWPVVASGGMS
                     IGHKGMTHAAKALAATMVDLFESEEHRRAIRAEFEEKTRGKTYKGYIPDGPPPVPAGV
                     Q"
BASE COUNT          489 a         1067 c         1092 g          495 t
ORIGIN      
        1 gccggcacgg gggcggacct cgcccacccg gccgttctgg tggccgtggt atcggtcctg
       61 gggaacctgg tagggaacgt gccggcagtg atgatgctcc tgcccttcgt ggaccgggag
      121 ccggtgaccg gctatgccct ggctctggcc agcaccttcg cgggcaacgc cgtgctggtg
      181 ggaagcatcg ccaacctgat cgtggccgag caggccgaga agctcggcat ccgcttcggc
      241 ttccgcgagc acctgcggac cggtctgccc gtcaccgtcg tctcgctcgc cgtggccgcc
      301 gcggccatgg cgctgtggta gagccggccg ggcccgctcc cttcactcct ttttcttcga
      361 tccctgggcc tcgcggcgcg gctccgcctc gagccggtgc ttcgatagga agtcccaagc
      421 catctggttg atcttcggcg cgagatcgta gtacccatga ttgtggccgg gtatcgatga
      481 tcgccatggg gatcttgcgc ttcgcggtta tggtcaggta cctggcggcg ggcagcagct
      541 tgccagcgtg gaccgcggtg gcggcgaagt actcggattc catgagggac aggatcagcg
      601 agtggacggc cccggcggag tggccgaaga gatagacccg gcgcgggtcg atgggatgct
      661 tcgccgtcct tggacgcggc ctccgccccc agggggagca gcacgagggc ggcggcccag
      721 aaaatcggca gtttcatcca cggtcctcct ttcgataagg gtgagattca gaccgaaagg
      781 atttcagccg cttcagtggg cccggagccc tctggccatg aggccgagcc cgccgaaaag
      841 gaggaccagc atgatcaggg tgttgaacag gctggtgggc tcgaaataaa tgttgaggtt
      901 cgcaatgatc cccgccagga tgatcaccac gccggcgatc aggagcagcc atcccagctt
      961 ggatttgccg ttgaagaaga gcagggccac gccggcgatg aacggcacca gcgacaggcc
     1021 gaaggcgttg tagccccaga gggtccagtg gccggagacg accgtcacgt gggtggtgag
     1081 caggtacccc ccggccacgg ccatgacgaa acccaccagg aactccccca gtccacccgg
     1141 agtgcctccc gcgccttcga gctgacgact catcggttct cctccaacgc tcggccgagc
     1201 gtccggatcg cgttcacata gatgtcggcc cgccgctcgg cggcgaggtc gaaggcgagc
     1261 atcagccccg cctggagagc aagcccccgc gagacggcat agagcgtctg gcggtcgcgc
     1321 aggaggaaca gcccggcgac gccggccgcc atcagtacga tctcgatcac cttgtaaatc
     1381 tggaagctgc gctgcacctt atccatccgc gccagctcgg cggcggcgta gcccccggga
     1441 tcgccctgga ggtgggtatg gagggagcgg gtctggccgt cggtgcgggc gaagacggtc
     1501 cagcccacca cgagctggat cagcgcgatg gcgatcagcg ggtaggccat gccacgcagg
     1561 ggactcccct tccagagcag cacgaccgag gcgatgatgg cgagcacccc ggccgcgatg
     1621 aacagcacgc tctcccgctt ctcgcccgag aagtactgga tgaccgccac gtgcatctgg
     1681 ctgaccgggt tcatggtggc cggattctat ctccggcgca ggagcagatg gcggtacggc
     1741 ccgcgggcgc gctcgtcgtg ctcgacctcg aacggggcgc cggaccgctc caggatctcc
     1801 cccatccggc ggttggtcga ggtgaagacc acgtccagca gggcgaggcc gaggcggcgc
     1861 aggggagaga tcgggacgcc gtccggcagg aacttgtcga acaccgccag ccgcccgccc
     1921 ggccggagca cccgcgccgc ctcgcgcagg cagcggacgg gatcggggat cacctccagc
     1981 accatgtgga ggatcgccgc gtcgaacgag ccgtccggca gctcgagggc catgccgtcc
     2041 agccgccgga attcgacgcc cggccgcgcg tggaccttcg cctgggcgag catcgcggga
     2101 gtgaggtccg tcgccaacac ctccgcgaac gccgggatga acgggatgtc cgctccggtc
     2161 ccgcagccga tgatgatcac ccgctcaccc ggccgcaggc cgagcagctc tacggagcgc
     2221 cggcgctcgg cgtggaactt ggtgatccga tcgtagagtg gcgcgtacag cgtgtaccgg
     2281 acgcggtacc aggcgttgaa gggcatcgcc tattgaaccc ccgccggcac cggcggaggc
     2341 ccgtccggga tgtagccctt gtaggtcttg ccccgggtct tctcctcgaa ctcggcgcgg
     2401 atggcgcgcc ggtgctcctc gctctcgaag aggtcgacca tggtggcagc gagggccttg
     2461 gcggcgtggg tcatcccctt gtggccgatg gacatgccgc cgctcgccac caccggccag
     2521 gcgtgccagg gggcgcccgc cggcgccgtg gtcacggaga ggtggagcgt cggcacgttc
     2581 cagctcacgt ccgccacgtc ggtcgatccc ccctcgggcg ggccggggtt ctcgttcagc
     2641 ggctggatgg cgccgtccag ccccttgggc tcgacgttcg tcgccttctg gatggcgcgc
     2701 gcgaactcgt gctcctgctc ggtgaacgcg agcggcccga gccaggtcag gttggagtgg
     2761 atcagccgcg cgcccgccat gttcaccagc atctcgtagt cgcccgactg caccctgagc
     2821 tccccctcga cgcccgcggc cagcgccgcc cccttgacga tgtcccgcac ccgcacgagg
     2881 agctggtcga cgctggtgtg ctcggtgtct cgcaaccagc accagatctt cgccttctcc
     2941 gggatgacgt tcgggacgtc cccgccttcg gcgatcacgt agtgcatgcg gacggtcggg
     3001 cgcacgtgct cccgcatcag gttgagggcg tgggtgaaga tctccaccgc gtccagggcg
     3061 ctgcggccgt tccacgggtc gtaggcggcg tgggcggcct tgccccggaa ctcgaccgcg
     3121 aagtccacca gcgcctggct gct
//
LOCUS       DASPJI010000090        18416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_348684, whole genome shotgun sequence.
ACCESSION   DASPJI010000090 DASPJI010000000
VERSION     DASPJI010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18416)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18416
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348684"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2849
                     /locus_tag="VEL74_02375"
     CDS             <1..2849
                     /locus_tag="VEL74_02375"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYG61403.1"
                     /translation="VCTLYEMEETPEGDLFLAMALCEGETLKSRLARGPLDLSEALDL
                     AGQIAAGLAHAHSRSLVHRDVKPSNLMLTPAGPLKRVKIVDFGVAVRPTRPRLTREGS
                     TVGTLSYMSPEQLRGGSVDHRADVWSLGVVLYEMLTGELPFDAPTDEAYAAAVLHGEP
                     RPVHELRPGLPAWLWPPLARMLSRRPDLRPSRIEEAVRDLRAPGNPEALLAGTIPSAV
                     PEVHPRVAELFVGRRDELALLARHLLPEPTEMLPAVVCAIQGMPGLGKSYLADRFALD
                     HADDFPGGHLRLVLDPRTPAAPEALLEELADRLGVPGEEGLEERLRHRLCRPRVLLHL
                     ENVDSPAAEIVAGHLLRRLPGCTAVVTGRLHDLGLGLGWRPVRIAAFDEESALSQLWG
                     ELGWEPDGAERDGHRELVRSLGYLPLAIHLAAGHLRSGRSAAGFLRLLRQRRLAVEPA
                     DRTELSLGASGEARKVLAHSFELSLEIFREELGADAGPLLAGLQALGHAPLTGFGRSL
                     GAGLAGLPEDDFEELAFHAQKLGLLLPVPREERPDGAWRLHPLLAEHLAVGVDAAEVR
                     DRMTRWFLARLPAGEPADQGRRWQEVQRESTALTWWLPRMPAADQQRVQAVATLFAAT
                     NGPFHVWIEFCEAALQGALEPRQRADYLWTLCRVALRQGRLERAFQAAQEKLQADIEV
                     ENEFGAAVAWELIAEVRMRRGELDEAVAILQRQIQVFEKLGETRERAVAASRVAYALT
                     LQERFDEALTVLHDDVVPFFERTGDVRSRAVALGMIADVLEARGNLDTALRIRQTEEL
                     PVYESLGEARSKAITMGKIARALHARGEHEAALKIRQEQERPIYERLGETRELAVCLG
                     RIADIYRSLGRRDEAIRIRRSEQLPLLEQLGSAYELVAGQESLAQLHLERRRPGDREE
                     AVTLLHRALEPAVRLRLPAEPRIRALLAEHGLAL"
     gene            3561..4061
                     /locus_tag="VEL74_02380"
     CDS             3561..4061
                     /locus_tag="VEL74_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61404.1"
                     /translation="MSSGLTVEDVLGRLEKQLAFHQEQEAFHADQETHHRNQREHHAG
                     QAESLARHIHAFREAAQPALDLASVPLPAAPSTPDLEALRDTGRRPPVQQIVMDVIQS
                     LPPGEQFGRSKMMAEIERRHGQHLRQPMDPSRVSTALRRLAAYGWILLTRPGRPHQEA
                     LYVREA"
     gene            4491..5114
                     /locus_tag="VEL74_02385"
     CDS             4491..5114
                     /locus_tag="VEL74_02385"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HYG61405.1"
                     /translation="MPDLLFDGELRRTLEDHLSRFERRSAPAEGRLPASVAVVVLPDE
                     QGRAAVVLTVRAAGLRRHGGQWACPGGRRDPGEAAEAAALRELEEEIGLSLSPKHVLG
                     CLDDCPTRSGFVITPVVVWGTEEAELRPDPREVAEVHLLPVAELTPENVVLQPGTERG
                     RPVMALSLSLGLLFAPTAAILYQFAEVAVHGRETRVDHFEQPRFAWR"
     gene            5164..6213
                     /locus_tag="VEL74_02390"
     CDS             5164..6213
                     /locus_tag="VEL74_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012024692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYG61406.1"
                     /translation="MIPTVIETETRRRDILWILALLLLLPGAVRAQADRGQTYDARLS
                     GYEYPFPVRFHPVRTQQQVLEMAYMDVRPERANGRAVLLLHGKNFSGAYWERTIRALT
                     AGGYRVIVPDQIGFGKSSKPERFQFSFQDLAAHTRGLLEALEVPRVSVVGHSMGGMLA
                     TRFALMYPGATEKLVLVNPIGLEDWKRLVPYRTIDASFEAELEATPESVRNYMRDNYF
                     AGDWKPEYEPLVEVQAGWLRGPDRRRMAWISALTSDMVFTQPVLYEFPDLRQPVLLII
                     GQRDRTAIGKAWAPPEVKPKLGNYPELGRKAQAAIPQARLVAIDNAGHLPQVEAFERY
                     RSALEEFLAAGGGPK"
     gene            6251..6436
                     /locus_tag="VEL74_02395"
     CDS             6251..6436
                     /locus_tag="VEL74_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61407.1"
                     /translation="MGTEKVSLRLEEVHLAEAREVAGTRGLSSYVNRALRQQLQQDRV
                     ALSSPSWTKRTDRSNRT"
     gene            6657..6869
                     /locus_tag="VEL74_02400"
     CDS             6657..6869
                     /locus_tag="VEL74_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61408.1"
                     /translation="MKAVGSVPETLEIHGRIAGRLLGIARLAHTVDALVVAHAVEAGG
                     AQILTGDRDDLERLAAPHPEVLIHPL"
     gene            complement(6900..8417)
                     /locus_tag="VEL74_02405"
     CDS             complement(6900..8417)
                     /locus_tag="VEL74_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61409.1"
                     /translation="MAQLEESSRERLRFAGLVAAACLVLTLPRLLLHELWRDEAWLWL
                     VAIESRSVAELLPPLARSGQGYLFPLLSYLAAQVWTSWRMLQLVHLVLVAAAAFVFVR
                     WAPFGRRERALFALGYFPFYEYAVISRHYVAGALLVWLACAGTRSRRPALALGAALGL
                     LCQTTVYGYILALAIAGGWLLDRWLRRDQVAPIPRGEAAAGLGLGVAGAVAGIIQLIP
                     RAGTSFAPGWLLGWDPAQAGRALTMPWRAFVPLPRPVFHFWNTNVLDTWPALQTVAGL
                     LILGLAVALLWRRKVALAIFCAGAAGLLAFGYAKYLGVLRHHGHWWLLFAAALWMGGL
                     ATREERRSWRSRALLVLLIGHCAAAAYASWMDLRHPFSNGAATAELIRGQGLDRDPLF
                     GYREPPSASVALALGRPLYAPSRKVFTTRPDWGPEQRDLPLPEVRCAARELARREGRD
                     IVLVMTVDLPPWEELEAIGSRLGAIQNSEDYRLYWLRHGRLEATAGAAACGNVDP"
     gene            complement(8469..11117)
                     /locus_tag="VEL74_02410"
     CDS             complement(8469..11117)
                     /locus_tag="VEL74_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG61410.1"
                     /translation="MRALRNAMMRLSGSLRQRTAEPELEEEIQFHIDMQTAKNLRLGM
                     SPDEARRAALVSFGGRERFKEEARDEYRSRPVEDLVQDLRYGVRSALGAPLFSLLAVL
                     TLALGIGANAAVFGVVKSVLLDALPYADADRLVRVYSRLEDGSLARSPLSAGAVTDIA
                     RRQRSFSRLAPFMHWTPDLAYADDRGPQLVKGAMVGRGFFQTLGVRALLGRTLADADA
                     APGAPVVAVLGYDAWQRMFVGDPQVLGRTGSVNGTSFEVVGVLPRGFVGPMGEADLWI
                     PLDLGPALSDPVRARQSHWLGLVGRLAPGATVEGAQREVDTIAAALAREYPDSDAGRL
                     FTSEPLRDSMVGDTRTPLLVLMASAGLVLLITCANLAGALLSRALSRRKEFAVRVALG
                     AGRGRLVRQLLTESAVLALAGGAAGILLAALGLAALRELALTVLPAYADLTLDGGAVL
                     VTSVLALCTGFAFGVVPALSVSRSNPQGMLRDESRGTSETKRSRRLRGVLVAGQIALS
                     VSLLAGAGLLARSLWAMTTAPLGFDPEGVLTVGVSLPPSEYTTPEASGRLYQQLEERL
                     RALPGVTGVGSVSELPAPSMDRNGLTIEGETWPPEAGPPFIITTSVSDGYFQTMGIPL
                     LRGRTFGTSDRPDTPHAIVISEGMARRYWPGGRALGARIRLGNASAPWAEVIGIVGNV
                     RNDPALPDPEPMAYASLRQDPWGSRTVLVRTEGDPLALVRPFQRELAALDPGIPTRDP
                     MTLRAYLADGLAGRRLPVVLMTAFGALALLLASVGVYAMFASMVAAREWEFGLRVTLG
                     SPRRAIAALVLWAGAAWMAAGLAGGAVGVVVVARLLRNLLYGVPPFDPVALGASVAIL
                     LACGTVALLVPVRRATRVDPITAIKV"
     gene            complement(11130..11495)
                     /locus_tag="VEL74_02415"
     CDS             complement(11130..11495)
                     /locus_tag="VEL74_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HYG61411.1"
                     /translation="MTDKNTDKITDKNRGDLLQGTLDMLVLKALQLEPMHGWGITERI
                     EQWSQKVLQVNQGSLYPALYRLQRQGWITSSWKTTENNRRARYYELTAAGRAQLAAEQ
                     ESWERLSEAVNLVLRMSAT"
     gene            complement(11583..18416)
                     /locus_tag="VEL74_02420"
     CDS             complement(11583..18416)
                     /locus_tag="VEL74_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61412.1"
                     /translation="MQAIAAEPPPGFPVIDLGGLTESVRREELARLALAEGRRPFDLA
                     CGPVFRTALLRLSQSEHSLFLNVHHIASDGWSLGVLHRDLAALYGAFHRRQGSPLPAL
                     PVQYADFAVWQRGWLQGEALRARLDYWRGRLADMPEVLELPTDRPRPAIESFRGAGAA
                     FPLAAPLVRRLSLLARRRGATLSMVVLAGFKVLLSRLSGRGDIPVGVAIAGRNRRETE
                     ELIGFFVNTLVLRTRLEDAPGFGDLVARVRETSLGAYAHQDLPFEKLVEELAPDRDLG
                     RNPLVQVTFGFHNFTSREVETGGLVLIPPAEEAVGTGTSKVDLSLHLFEGGGALHGFL
                     EYNLDLFDEATARRFTGYLPALLAAAVEDPERPVGEIPLLGEAETHQLSREWNDTATA
                     LPAEPVHRLFERRAAAVPSAVAVAFMGEELSYRELDRRANRLAHHLIALGVRPGAPVA
                     LRLERSVEIAVAMLGVWKAGGAFVPLDPEAPRPRLEGMLREVFGDATPIVVDREQVAA
                     AMASGRDGRPRVEVGPESAAYLIFTSGTTGRPKAVLVDHGSLANVLSVIQRDLDWDGR
                     DRMPVLAAYSFDIFLFELWNPLLAGGRAELFALRPVPDVRRLTEALPAMTRLHAVPSL
                     MREIVEEIRSQAGRISGLRTVFVGGEAVPPDLLADLLEVFPGAEVRVLYGPTEGTILC
                     ASHPVDGQALARPPLGRPLGNTLLELRDRRGQQVPLGVAGEIWIGGAAVSRGYWNRPE
                     LTRERFVDGWYRTGDLARYRADGVLEYLGRADEQVKVRGFRIEPGEVEEALREHPAVR
                     EAVVVSREDEPGQRRLVAYLTCEPVEAQRLVPRLRSFLEQRLPDYMVPSALVVLDRLP
                     RTAHGKLDRRALPAPERLRAELEETFVAPRTAEEEILAGIWSQVLGLDRVGARDNFFA
                     LGGHSLLATQVMSRIRQLFGVEVELRSLFEVPTVEGLAGRVREVLAGGPAAVPPVERV
                     PRPGPLPLSFGQQRLWFLERLEPGAAYNMPSAFRLLGDLDDAAWERAGGEILRRHEVL
                     RTAYREIAGEPVQVIEPPLPLTLPRVDLSALPAAPRQAEARRLGAEEARRPFDLARAP
                     MLRTLLLRLAPREHLVLFTLHHIAGDGWSMEVLARELAALYEAFATGHPSPLPELPIQ
                     YADFAVWQRRWLEGPVLEAQLAYWKEHLAGAPPSLDLPMDRPRRPLGGARAGSFRGVE
                     LPAEVAQSLERLRGREGSTLFGALLAAFQALLSRYSGQVDISVGTPVAGRRQMETEDL
                     IGFFVNTLVLRTDLEGDPSFRELLGRVRSVTLGALSHQELPFERLVEELQPERSLAHS
                     PLFQAMFALQMLPAKPPRLPRLTFAPAEIDAASARFELELTVMEPAPGGDDPLSRTGL
                     PVGVDYNADLFDDSTIERLARHFALLLEGAVADPERRLSELPLLTDAERRQLVEWNRT
                     AVPLPTVPVRELIRERARQAPENPAVILDGGVMSYGELDGRASRLAAFLRAAGIGPEI
                     PVGVHLERSPALYVALLATFEAGGCYLPLDPDLPPERLQRILASSGTPLVLTEERLAD
                     RLPPLPPQTRRVCLDGEWDRIEATGTGPAAALVDPGSLAYAIYTSGSTGEPKAVGIPH
                     RALTNHDLVTSARFGLGPDDRVLQFAAIGFDLGLEEMLPTWVSGGAVVPRPAGTFPAF
                     EEFDGLLRRYGITVLNMSTGYWHEWVASMVRSRRLPPPSLRLVVIGTEQALPEVVAAW
                     RRHVQGRIRWINAYGATETINSTTVYEDAPGHPEHRVPIGSPLPNVQAHVLDAALEPV
                     PVGVVGEICIGGADLARGYLGRPDWTAASFRPDPWAAEPGGRLYRTGDRGRRLPDGSL
                     EHLGRVDVQVKVRGFRVEPGEVEAVLLRHPAVEECAVVAQGEPGAQRLAAFIVARSGE
                     AGDPLAAELRAFTAAALPAYMVPSTFSRLERLPLTPSAKVDRRQLAAAPTAPEAGLAA
                     SAIPPRDATERRLAEIWRELLGLPTVGIEDSFFDLGGHSMLAVRLMSRIRGDFGRDLP
                     ISALFRLPTIAGLAGALREEAAPPSDSALVEIQPGGGARPLFLVHPSGGNVFSYADLA
                     RKLGGERPVFGLQARGTEAGGDGLDTVEKMAAHYLREVRRAQPAGPYLLGGWSFGGLV
                     ALEMAHQLREAGEEAGLVALIDPSPPNPPGWPARETGEAEMAREFARDLAGLAGPEAD
                     PEQLRRYFEVFQRSVRAHSVHVPRPYPGRVALLATEEGMRLFDPLRLWKPLLSGPLET
                     RVFASDHYSILKSPVVGEVAAWLQELLQD"
BASE COUNT         2857 a         6615 c         6506 g         2438 t
ORIGIN      
        1 acgtctgcac cctctatgag atggaggaga cgccggaggg ggacctgttc ctcgccatgg
       61 cgctctgcga aggcgagacc ctgaagagcc gcctcgcccg cgggccgctg gacctctccg
      121 aggcgctgga cctggccggg cagatcgccg ccggcctcgc ccacgcccat tcccgcagcc
      181 tcgtccatcg cgacgtcaag ccgtccaacc tcatgctcac cccggccggc cccctgaaaa
      241 gggtgaagat cgtggacttc ggggtcgccg tgcggcccac ccggccccgg ctcacccggg
      301 aaggctcgac cgtgggcacc ctctcctaca tgtcccccga gcaactccgc ggcgggtccg
      361 tggatcaccg cgccgatgtc tggtcgctcg gcgtggtcct ctatgagatg ttgaccggcg
      421 agctgccgtt cgatgccccc acggacgagg cctacgccgc cgcggtcctc cacggggagc
      481 cccgtcccgt ccacgaactg cgccccgggc tgccggcgtg gctctggccg ccgctcgcca
      541 ggatgctctc ccgccggccg gacctccgcc cgtcgcggat cgaggaggcg gtgcgggacc
      601 tccgtgctcc cggcaacccg gaggcgctgc tcgccggcac gatcccctcc gccgtcccgg
      661 aggtccatcc gcgggtcgcc gagctgttcg tcgggcgccg cgacgagctc gccctgctgg
      721 cccggcacct cctgccggag cccaccgaga tgctgccggc ggtggtctgc gccatccagg
      781 ggatgcccgg cctgggcaag tcctatctcg cggaccgctt cgccctcgac cacgccgacg
      841 acttcccggg cggtcacctc cggctcgtcc tcgatccgcg aacccccgcg gcgccggagg
      901 ccctgctgga agagctggcc gaccggctgg gggtgccggg cgaggagggc ctggaggagc
      961 ggctgcggca caggctgtgc cggccccgag tcctgctcca tctcgagaac gtcgactctc
     1021 cggcggccga gatcgtcgcc gggcacctcc tccgccgtct gccgggctgc accgccgtgg
     1081 tcaccggcag gcttcacgac ctggggctcg gcctcggctg gcgaccggtg cggatcgcgg
     1141 ccttcgacga ggagagcgct ctttcccagc tctgggggga gctgggctgg gagcccgacg
     1201 gcgccgagcg cgacggtcac cgcgagttgg tgcggagcct ggggtatctg ccgctggcca
     1261 tccatctcgc ggccgggcac ctgcggtcgg ggcgctcggc ggccggcttc ctgcgcctgc
     1321 tgcggcagcg gcgcctggcc gtcgagccgg cggaccggac cgagctgtcc ctgggagcct
     1381 ccggtgaggc gcgcaaggtc ctcgcccaca gcttcgagct gtcgctggag atcttccggg
     1441 aagagctcgg cgccgacgcc ggccctctgc tcgccgggct ccaggccctg gggcacgcgc
     1501 cgctgaccgg cttcggccgc agcctgggtg ccgggctggc cggcctgccg gaggacgact
     1561 tcgaggagct ggctttccac gcgcagaagc tggggctcct cctgccagtc cctcgggagg
     1621 agcggcccga cggcgcctgg cggctccacc ccctcctcgc cgagcacctg gccgtcgggg
     1681 tggacgccgc ggaggtcagg gaccggatga cccggtggtt cctggcccgc ctgccggccg
     1741 gagagccggc ggatcagggg cgccggtggc aggaagtaca gcgggagagc accgccttga
     1801 cctggtggct gccgcggatg cccgccgccg accagcagcg ggtgcaggcc gtcgcaacgc
     1861 tctttgccgc caccaatggc ccttttcatg tatggatcga gttctgcgag gcggccctcc
     1921 agggagcgct cgaaccccgg caacgggctg actatctctg gactctctgc cgcgtcgcgc
     1981 tgcgtcaagg gcgcttggag agagcgttcc aggccgcgca ggagaagctc caggccgata
     2041 tcgaggtgga gaatgagttc ggggccgctg tcgcctggga gctcatcgcc gaggtccgga
     2101 tgcggcgagg agagctcgac gaagccgtgg caatcctcca aaggcagatc caggttttcg
     2161 agaagctcgg ggagacccga gaacgggctg tcgccgccag ccgagtcgcc tatgccttga
     2221 cgcttcagga acgattcgat gaagcgttga cggtcctgca cgatgacgtc gtgccctttt
     2281 tcgagaggac aggggacgtc cgctcgcgtg cggtggccct gggcatgatc gccgatgtcc
     2341 tggaagcccg agggaatcta gacaccgcat tgcgcatccg ccagacggag gagctccccg
     2401 tctatgagag tctgggagag gcccgctcca aggcgatcac catgggcaag atcgcaaggg
     2461 ccttacatgc gcgcggcgag catgaagcag cgttgaagat ccggcaggag caggagcggc
     2521 cgatctatga gcggctgggc gagacccggg agctggcggt ctgcctgggg cggatcgccg
     2581 acatctaccg cagcctgggg cggagggatg aggcgatccg gatccgccgg agcgagcagc
     2641 tcccgctcct cgagcagctc ggctcggcct acgagctggt cgccgggcag gagagcctgg
     2701 cccagctcca cctggaacgc cggcggcccg gcgaccgaga ggaggccgtg accctcctgc
     2761 accgtgccct cgaacccgcc gtccgtctcc gccttcctgc cgaaccccgc atccgggcgc
     2821 tgctggccga gcacggcctc gcactgtaaa gccagcggag caggtcggct cgacttgctg
     2881 agccgcggca tagccttgcg catagacact ggcctatcca gcgaggcccc ccacctcttc
     2941 ctggactcca cctcctcttc tctttgacgc gaaccctgat ccagccgaca ccaattctcc
     3001 ctcataggcg tcggcgacct ccctcttcac gtcgaatgag taggcacccg acgctaaatt
     3061 ttcgatctcc gccgcgaatc ctgctagagt cgactcgatc ctggcaggtg ctggcccgaa
     3121 caccggaagc tgcattcggt tcacttcggc ggccgacacc tgccgactca gggaggacgc
     3181 gaacctggcg gcttccgacg cgaaaccagt cccgtccgac accaagatct acactcttga
     3241 cgtccatcaa acctctttcg cgtcggtcga aacgagagcc gacgcaaagc cttcgagggc
     3301 gactccatcc gccggtacag ccgacgccaa tcgaagagag atcgcgtccg acaggggtgg
     3361 gtgaacgtgg actgaagtgg tggagccact ctgctctccg ggaggagatc ctcctcgagc
     3421 gggagcaggc gtcagagcat cggacgctga tgtcagggat ggcaccttgg acgcgaagag
     3481 ctgttgcttc gacgcgaacc cagaagcatt ggacgtgaat caggcggcgc cgagagcgcc
     3541 ggaacagagg aaggaacggg atgagctcgg gattgacggt cgaggacgtg ctggggcgcc
     3601 tggagaagca gttggccttt catcaggagc aggaggcctt tcacgcggac caggagacac
     3661 atcaccggaa ccagcgggag catcatgccg gccaggccga gagccttgcc cggcacatcc
     3721 atgcgttccg tgaggccgcg cagcctgcgc tcgatctcgc cagcgtgccg ctgccggcgg
     3781 cgccgtccac gccggacctg gaggccttgc gggacaccgg ccggcgcccg ccggtccagc
     3841 agatcgtgat ggacgtgatc caatccctac cacccggcga gcagttcggc cggtccaaga
     3901 tgatggccga gatcgagcgg cggcacggcc aacatctgcg gcagcctatg gaccccagcc
     3961 gcgtttccac cgccctgcgc cgcctggcgg cttacggctg gatcctcctg acgcgccccg
     4021 gccgtccgca tcaggaggca ctgtatgtgc gggaagcttg atgcccggtc cggcccttgc
     4081 agacgcggcc cggaaccttg gcgggaggtg gagcggcccg accgcaccct cccgcagccc
     4141 gcggccgaag tgaatgctcg cctgccgggg gctcaggcac ctgaccttgg gagagcttcg
     4201 atccaggagc tgtcagcttg aaagcgagga gttgcccatg aagcgagccc tacgagctct
     4261 aggtcttttt ggaatcctct cgatggtgac ggccctatcc gacagcccga aggccatggc
     4321 cgtcatcctg tgcgagaaca tccacggcac ctactgtgct ccgggcagcc gcggaggagt
     4381 gtgcttcggt tccagcaatc cggatgtcaa cgatgccggc ttctgcgtat gcgtagccag
     4441 caagtggcag tgtagttggt gaggtaacgg agctgcctcg gaagggcggc atgccggacc
     4501 tgctctttga cggcgagctg cgccgcaccc tggaggatca cctgtcccgc ttcgagcggc
     4561 ggagcgcgcc ggcggagggg cgcctgccgg cttcggtggc cgtggtggtg ctgccggacg
     4621 agcaggggcg ggcagcggtc gtgctcacgg tgcgggccgc cgggctgcgc cgccacggcg
     4681 ggcaatgggc ctgcccgggg ggccggcgcg atcccggaga ggctgccgaa gcggcggcgt
     4741 tgcgggagct tgaggaggag atcggactgt ccctgtcccc gaagcacgtt ctcggctgcc
     4801 tggacgactg cccgacgcgc tccggcttcg tgatcacccc cgtggtggta tgggggaccg
     4861 aggaggccga gctacgtccc gatccccggg aggtggcaga ggtccatcta ctgccggtgg
     4921 cagagctgac gccggagaac gtggtcctcc agcccggcac cgagaggggc cgtccggtca
     4981 tggcgctgtc gctctccctt ggcctcctct tcgcccccac cgccgcgatc ctttatcaat
     5041 tcgccgaagt ggccgtccat ggccgggaga cgcgagtcga tcacttcgag cagccgcggt
     5101 tcgcgtggcg gtaggctccc gggaagccat cttgcaggat cctcccgcga ggagggcctg
     5161 ctcatgatcc cgaccgtcat cgaaaccgaa acccgccgca gggacatcct ctggatactc
     5221 gctctgctgc ttctcctgcc cggagccgtc cgggcgcagg ctgaccgggg ccagacctac
     5281 gacgccaggc tgtccggtta cgagtacccc ttcccggtgc gatttcatcc ggtgaggacg
     5341 cagcagcagg tgctggagat ggcctacatg gacgttcgtc ccgagcgggc caacggcagg
     5401 gccgtgctgc tgctccacgg caagaacttc tccggcgcct actgggagcg gacgatccgg
     5461 gccctgaccg ccggcggcta ccgggtgatc gtccccgacc agatcggctt cggcaagtcg
     5521 tcgaagccgg agcgctttca gttcagcttc caggatctcg ccgcccacac ccgcgggctc
     5581 ctcgaagctc tcgaagtgcc gcgcgtgtcg gtggtgggcc actccatggg cggcatgctg
     5641 gcgacccgct tcgccctgat gtatcccggc gccaccgaga agctggtcct ggtcaacccg
     5701 atcggactcg aggactggaa gcggctcgtc ccctaccgca cgatcgacgc cagcttcgag
     5761 gcggagttgg aggccactcc cgagagcgtg cgcaactaca tgcgggacaa ctacttcgcc
     5821 ggcgactgga aacccgagta cgagccgctg gtcgaggtgc aggccggttg gctgcgcggg
     5881 cccgaccggc gccggatggc ctggatctcc gccctgacct ccgacatggt gttcacccag
     5941 ccggtgctct acgaatttcc ggacctgcgg cagccggtcc tcctgatcat cggccagaga
     6001 gaccgcacgg ctatcggcaa ggcctgggcg cctccggagg tgaagccgaa gttggggaac
     6061 taccccgagc tcggccgcaa ggcccaggcc gccatccccc aggcccggct ggtcgcgatc
     6121 gacaacgccg gtcacctgcc ccaggtcgag gccttcgagc gttaccgaag cgctctggag
     6181 gagttcctgg cggccggagg tggtccgaag taagactgct cctgaatctt acctggagta
     6241 ggatttcggc atgggcaccg agaaggtctc cctcagactg gaagaagtgc acctcgccga
     6301 ggctcgcgag gtggctggca cgcgcggctt gtccagctac gtcaatcggg cgctccgcca
     6361 gcagttgcag caggatcgcg ttgccctttc ctcgccgagc tggacgaaga gaacggaccg
     6421 gtcgaaccgc acgtgatgaa tgaggtccgt caggcatggc cggccccggg gagagatccg
     6481 ccaagcgccg cagcgcctga tcctggatgc cagagcggtc atcgctctcg cccggggaga
     6541 ccagagggcc cgggcgttcc tcgcccgcgc gctggagctg ggagccccca ttgagatccc
     6601 ggtggccgtg gtagccgaaa cggggaggac cgcgagatgc tcccgtcaac agggtcttga
     6661 aagcagtcgg ctcggtaccc gagacgctgg aaatccacgg gaggatcgcc ggccggctcc
     6721 tcggcatcgc cagattggcc cataccgtcg atgccctcgt cgtcgctcat gccgtcgaag
     6781 ccggaggagc ccagatcctc accggggacc gcgacgatct cgaacggctc gccgcacctc
     6841 accccgaggt cctgatccat cctctctgaa ggatctctcc cgcccgcagc cggctcaggc
     6901 tacggatcga cgttcccgca cgccgccgcc ccggccgtgg cttccaggcg gccgtgacgc
     6961 agccaataca gccgatagtc ctcggaattc tggatcgcgc cgagcctaga tccgatggcc
     7021 tccagctcct cccagggcgg gaggtcgacg gtcatgacga ggacaatgtc ccggccctcc
     7081 cgccgcgcca gctcgcgggc ggcgcaccgc acctcgggca gagggaggtc ccgctgctcg
     7141 ggcccccaat ccggacgggt ggtgaagact ttccgcgacg gagcgtagag cggccggccg
     7201 agcgcgagcg ccaccgaagc cgacggcggc tcgcggtatc cgaacaacgg atcccggtcc
     7261 aacccctggc cgcggatcag ctccgcggtc gcggcgccat tcgagaaggg atgccggaga
     7321 tccatccaac tggcgtaggc cgcggcggca cagtggccga tcaggaggac caggagagcc
     7381 cgcgagcgcc aggagcggcg ctcctcccga gtagccagtc cccccatcca gagggccgcg
     7441 gcgaagagca gccaccagtg gccatggtgg cgcaggacgc cgagatactt cgcgtacccg
     7501 aaggcgagca ggccggcggc gccggcacag aagatcgcca aggccacctt ccggcgccag
     7561 agcagggcga cggccaatcc caggatcagc agccccgcca ccgtctgcag agcgggccag
     7621 gtgtcgagga cgttggtgtt ccagaagtgg aagacgggcc ggggcagggg cacgaaggcg
     7681 cgccagggca tcgtcagcgc cctgccggcc tgagccggat cccatccgag aagccagccc
     7741 ggcgcgaaac tggtaccggc tcgggggatg agctggatga tccccgcgac ggcgccggcc
     7801 acccccaggc ccaggccggc ggcggcctcg ccgcgtggga tcggtgccac ctgatcacgg
     7861 cggagccagc ggtcgagcag ccatccgccg gcgatggcga gggccaggat gtagccgtag
     7921 acggtggtct ggcagagcag cccgagcgcc gcgccgagag cgagggccgg ccggcggctg
     7981 cgggtcccgg cgcaggcgag ccagacgagc agcgcgccgg cgacgtagtg gcggctgatc
     8041 accgcgtatt cgtagaacgg gaagtagccc agcgcgaaga gcgcgcgctc gcggcggccg
     8101 aacggcgccc agcggacgaa gacgaaggcc gcggcggcga ccaggacgag gtgcacgagc
     8161 tggagcatcc gccacgacgt ccacacctgc gccgccaggt aggagagcag ggggaagaga
     8221 tacccctgac cgctcctcgc cagaggcggc aacagctcgg cgacggagcg gctctcgatg
     8281 gcgaccagcc agagccaggc ctcgtcccgc cacagctcgt gcaggagcag gcgcggcagg
     8341 gtcaggacga ggcaggccgc ggcgacgagc cccgcgaaac gcagacgctc ccgggacgac
     8401 tcttccagtt gggccatggg ccgcaactat agccgcccgg gagcgcctcc ggcagcggcc
     8461 aaccgccgtc agaccttgat cgccgtgatc gggtcgacgc gcgtcgcccg gcggaccggg
     8521 acgagcagag cgacggtgcc gcaggcgagc aggatggcga cggaggcccc cagcgccacc
     8581 gggtcgaacg gcggcacgcc atagagcagg ttgcgcagaa ggcgcgcgac caccaccaca
     8641 ccgacagccc ctccggccag accggccgcc atccaggcgg cgccggccca cagcaccagc
     8701 gcggcgatgg cgcgccgggg ggagcccagg gtgacgcgga ggccgaactc ccactcccgc
     8761 gccgccacca tgctggcgaa catggcgtag acgcccaccg acgcgagcag cagcgccagc
     8821 gcgccgaacg cggtcatcag taccaccggc agccgccggc ccgcgagccc gtcggccaga
     8881 taggcgcgca acgtcatcgg atcgcgggtc gggatccccg ggtcgagcgc cgccaactcg
     8941 cgctggaagg ggcggaccaa ggccagcgga tctccctccg tacgcaccag gacggtccgg
     9001 ctcccccatg gatcctgccg gagggaggcg taggccatgg gctccgggtc cggcagggcg
     9061 ggatcgttgc gcacgttccc cacgatgccg atgacctcgg cccagggcgc gctcgcgttg
     9121 ccgagccgga tacgcgcgcc cagcgcccgt ccgccgggcc aatagcgccg cgccatgccc
     9181 tcgctgatca cgatggcgtg gggcgtgtcc ggccggtcgg aggtcccgaa ggttcgcccg
     9241 cgcagcagcg gtatgcccat ggtctggaag tagccgtccg agacgctggt ggtgatgatg
     9301 aacggcggcc cggcttccgg cggccaggtc tcgccttcga tcgtgagccc gttccggtcc
     9361 atggacggcg cgggcagctc gctcacgctc cccacgccgg tgaccccggg gagggcacgc
     9421 aggcgttcct cgagctgctg gtagaggcgg ccggacgcct ccggcgtcgt gtactcgctc
     9481 ggcggcaggc tgacgccgac cgtcagcacg ccctccgggt caaagccgag cggcgcggtg
     9541 gtcatcgccc acaggctgcg ggcgagcaga cccgcgcccg cgaggaggct gacggagagc
     9601 gcgatctgcc ccgccacgag cacgccgcgc aggcgccgcg accgcttcgt ctcgctggtg
     9661 ccgcgcgact cgtcgcgcag catgccttgc gggttcgagc ggctgacgga cagcgccggt
     9721 accacgccga aggcgaagcc ggtgcacagc gcgagcaccg aggtgacgag caccgcgccg
     9781 ccgtcgaggg tcaggtccgc gtacgccggc aggaccgtca gcgcgagctc gcgcaacgcg
     9841 gccagcccga gcgccgcgag caggatccct gccgcgccgc cggcgagcgc cagcaccgcg
     9901 ctctcggtga gcagttgccg gacgagccgg ccgcggccgg cgccgagcgc cacgcgcacg
     9961 gcgaactcct tgcgccggga gagggcgcgg gacagcagcg ctccggccag gttcgcgcag
    10021 gtgatgagga gcaccagccc ggcgctcgcc atcagcacga gcagcggagt gcgggtatcg
    10081 cccaccatgg agtcgcggag cggctccgag gtgaagaggc ggccggcgtc ggagtcgggg
    10141 tactcgcgcg ccagcgcggc ggcgatggta tcgacctcgc gctgggcccc ctcgacggtg
    10201 gcgcccgggg ccaggcggcc gaccaggccc agccagtggc tctgccgcgc gcggaccggg
    10261 tcgctcaacg ccgggccgag atcgaggggg atccagaggt cggcctcccc catcggcccg
    10321 acgaagccgc gcggcagcac tccgaccacc tcgaaggagg tcccgttgac gctgccagtc
    10381 cggccgagca cctgcgggtc gccgacgaac atccgctgcc aggcgtcgta gcccaggacc
    10441 gccaccaccg gcgcgcccgg cgcggcgtcg gcgtccgcga gcgtgcgccc gaggagggcc
    10501 cgcacgccga gcgtctggaa gaagccgcgc ccgaccatcg cgcctttcac gagctgcggc
    10561 ccgcggtcgt cggcgtaggc gaggtcgggc gtccagtgca tgaagggggc gaggcgggag
    10621 aacgaccgct ggcgtctggc gatgtccgtc acggcgccgg cgctcagcgg cgagcgcgcg
    10681 agcgatccgt cctcgaggcg cgagtagacg cgcacgagac ggtccgcgtc ggcgtagggc
    10741 agcgcgtcga gcagcaccga cttcaccacg ccgaagaccg cggcgttggc accgatgccc
    10801 agggcgaggg tgagcaccgc caggagcgag aagagcggcg cgcccagtgc cgaccgcacg
    10861 ccgtaccgga ggtcctggac gaggtcctcg acgggacggc tgcggtactc gtcccgcgcc
    10921 tcctccttga agcgctcgcg cccgccgaag ctgacgagcg ccgcgcggcg tgcctcgtcc
    10981 gggctcatcc cgagacggag gttcttcgcc gtctgcatgt cgatgtggaa ctggatctcc
    11041 tcctccagct cgggctccgc cgtccgctgc cggagcgagc cggacagcct catcatcgcg
    11101 ttccgcaaag ctctcatggt gctgctcctc taggtcgcgg acatccgcag gacgaggttc
    11161 acggcctcgg agagccgctc ccagctctcc tgctcggcgg cgagctgggc gcgtccggcc
    11221 gcggtgagct cgtagtagcg cgcccggcgg ttgttctccg tcgtcttcca ggaggaggtg
    11281 atccagccct ggcgctgcag ccggtagaga gccggataga gcgacccctg gttgacctgc
    11341 agcaccttct gggaccactg ctcgatgcgc tcggtgatgc cccagccgtg catgggctcg
    11401 agctggagcg ctttcagcac gagcatgtcg agggtgccct ggagcaggtc gcccctgttc
    11461 ttgtcggtaa tcttgtcagt gttcttgtcc gtcatgagcc gtccattcct ctcgatgttc
    11521 tagaggaatg gtgcggcgct tcctctcgat tgtcaagagg aagggtagga ggaggggaag
    11581 agctagtcct gcaggagctc ctgcagccag gcggccacct cgccgaccac cgggctcttc
    11641 aggatggagt agtggtccga ggcgaagacc cgggtttcca ggggaccgga gagcaggggt
    11701 ttccagagcc ggagagggtc gaagaggcgc attccctcct ccgtcgccag cagggccacc
    11761 cggccgggat agggccgcgg cacgtggacg ctgtgcgccc ggacgctgcg ctggaagacc
    11821 tcgaagtagc ggcggagctg ttcagggtcc gcctcgggac cggcgagccc ggcgaggtcc
    11881 cgggcgaatt cgcgggccat ctcggcctcg cccgtctcgc gcgccggcca gccgggaggg
    11941 ttcggcgggg acggatcgat gagggcgacg agcccggcct cctcccccgc ctcgcggagc
    12001 tgatgagcca tctccagggc caccagcccg ccgaacgacc agcctccgag gaggtacggc
    12061 cccgcgggct gagcccggcg gacctcgcgg aggtagtggg cggccatctt ctcgacagta
    12121 tcgaggccgt cgccgcccgc ctcggtgccg cgagcctgca gcccgaagac cggtctctcc
    12181 ccccccagct tgcgggccag atccgcgtag ctgaagacgt tgccgccgga gggatggacg
    12241 aggaagagcg gacgcgcgcc gccgccgggc tggatctcca ccagagcgga gtcagaaggc
    12301 ggcgccgcct cctcgcgcag ggcaccggcc agccccgcga tggtgggcag gcggaacagc
    12361 gcggagatgg ggaggtcgcg gccgaagtcg ccgcggatcc gtgacatcag acggaccgcc
    12421 agcatggagt gcccgcccag gtcgaagaag ctgtcctcga tccctaccgt cggcaggccg
    12481 aggagctcgc gccagatctc ggccagccgc cgctcggtcg cgtcgcgggg agggatggcg
    12541 gaggcggcga ggccggcctc gggcgcggtg ggagcggcgg cgagctgccg gcggtccacc
    12601 ttggcgctgg gagtcagcgg cagcctctcc aggcgcgaga acgtcgacgg caccatgtag
    12661 gcgggcagcg ccgcggccgt gaaggccctg agctcggccg ccagcggatc cccggcctcc
    12721 ccgctccgcg ccacgatgaa agccgccaga cgctgcgccc ccggctctcc ctgggcgacg
    12781 acggcgcact cctccaccgc tggatgacgc agcagaaccg cctcgacctc ccccggctcc
    12841 acccggaaac cgcgcacctt gacctgtacg tcgacacgcc ccaggtgctc gagggagccg
    12901 tccggcaggc ggcggccccg gtcgccggtg cgatagagcc gacctcccgg ctcggcggcc
    12961 caggggtccg gccggaagct cgccgcggtc cagtccggcc gtcccaggta gccgcgcgcc
    13021 aggtcggcgc cgccgatgca gatctccccc accacgccga ccggcaccgg ctccagcgcg
    13081 gcgtccagca cgtgcgcctg gacgttgggc agaggcgagc ctatggggac ccggtgctcg
    13141 ggatgcccgg gagcgtcctc gtacaccgtg gtcgaattga tggtctccgt ggccccgtag
    13201 gcgttgatcc accggatccg tccctggacg tgccggcgcc acgcggcgac cacctcgggc
    13261 aacgcctgct cggtgccgat gaccaccagg cgcaggctcg gcggcggcag gcgtcgcgag
    13321 cgcaccatgc tcgccaccca ttcgtgccag tagccggtgg acatgttgag cacggtgatc
    13381 ccgtaccgcc gcagcagccc gtcgaactcc tcgaaggccg ggaacgtgcc ggcgggcctc
    13441 ggcaccaccg cgccgccgct cacccaggtg gggagcatct cctccaggcc caggtcgaag
    13501 ccgatagcgg cgaactggag cacccggtcg tccggcccca ggccgaagcg ggcgctggtc
    13561 accaggtcgt ggttcgtcag cgcccggtgc gggatgccca ccgccttggg ctctcccgtg
    13621 gagcccgagg tgtagatggc gtacgccagg ctccctggat cgaccagcgc cgcggcgggc
    13681 cccgtgccgg tggcctcgat ccggtcccac tcgccgtcca gacagacccg acgcgtctgg
    13741 ggaggcagtg gaggcagtcg gtccgccagc cgctcctcgg tgagcaccag cggtgtcccg
    13801 gatgaggcca ggattctctg caggcgctcg ggaggcaggt cggggtcgag ggggagatag
    13861 cagccccccg cttcgaaggt ggcgagcagg gcgacgtaca gggccggcga gcgctccaga
    13921 tggaccccca ccggaatctc ggggccgata cccgccgccc gcaggaaggc ggcgagccgc
    13981 gacgcccggc cgtcgagctc gccgtagctc atgacgccgc cgtcgaggat cacggccgga
    14041 ttctccggcg cctgccgcgc ccgttcccgg atgagctcac ggaccggcac ggtgggtagg
    14101 gggacggcgg tgcggttcca ctccacgagc tgacggcgct cggcatcggt cagcaggggg
    14161 agctccgaca ggcgccgctc ggggtcggcc acggccccct ccaggaggag ggcgaagtgc
    14221 cgcgccaggc gctcgatggt gctgtcgtcg aacaggtcgg cgttgtagtc cacccccacg
    14281 gggagcccgg tccgggacag cgggtcgtct ccgcccgggg ccggctccat caccgtcagc
    14341 tcgagctcga acctggcgga ggcggcgtcg atctccgccg gcgcgaaggt gagccgcggc
    14401 agccgggggg gcttcgcggg cagcatctgg agggcgaaca tggcctggaa gagcggactg
    14461 tgggcgaggc tgcgctcggg ctggagctct tcgaccaggc gctcgaaggg gagctcctgg
    14521 tgactcaagg cccccagcgt caccgaccgc acccggccca gcagctcgcg gaagctggga
    14581 tcgccctcca ggtcggtgcg caggacgagc gtattgacga agaagccaat cagatcctcg
    14641 gtctccatct gccggcggcc ggccaccggc gtccccacgc tgatgtcgac ctggccgctg
    14701 tagcgcgaca ggagcgcctg gaaagcggcc agcagcgctc cgaacagggt gctcccctcc
    14761 cggccgcgca gccgctccag gctctgcgcc acctccgccg gcagctccac gccacggaac
    14821 gagccggccc gcgctccgcc cagcgggcgg cgcggccgat ccatcggcag gtccagcgac
    14881 ggcggcgctc ccgccagatg ctccttccaa taggcgagct gcgcctccag caccggcccc
    14941 tccagccagc gccgctgcca caccgcgaag tcggcgtatt gaatggggag ctccggcaaa
    15001 ggcgaagggt ggcccgtggc gaacgcctcg tacagcgccg ccagctctct ggccagcacc
    15061 tccatggacc agccgtcgcc ggcgatgtgg tggagggtga acagcaccag atgctcgcgg
    15121 ggggccagcc ggagcagcag cgtccgcagc atcggggcgc gggcgagatc gaagggccgc
    15181 cgcgcctctt cggctccgag ccgccgggcc tcggcctggc gcggcgcggc cggcagggcg
    15241 gacaggtcca ctcgcggcag tgtcaatggc agcggcggct cgatcacctg caccggctcg
    15301 cccgcgatct cccggtaggc ggtgcgcagc acctcgtggc ggcgcaggat ctcgccgccg
    15361 gcccgttccc aggccgcatc gtccagatcc cccagcagcc ggaacgccga aggcatgttg
    15421 tacgccgccc ccggctcgag ccgctcgagg aaccatagcc gctgctggcc gaacgacagc
    15481 ggcaacgggc ccgggcgcgg cacgcgctcg accggcggca ccgcagcggg tccgccagcc
    15541 agaacctcgc gcacccggcc ggccagcccc tccaccgtcg gcacctcgaa caggctgcgc
    15601 agctccacct ccacgccgaa gagctggcgg atgcgcgaca tcacctgggt ggccagcaga
    15661 gagtggccgc ccagggcgaa gaagttgtcc cgcgcgccga cccggtcgag ccccagcacc
    15721 tgcgaccaga tccccgccag gatctcctcc tcggcggtgc ggggcgccac gaaggtctct
    15781 tccagctcgg cccgcagccg ctccggtgcc ggcagcgccc gccggtccag cttgccgtga
    15841 gcggtccgcg gcagccggtc cagcaccacc agggccgatg gcaccatgta atcgggcagc
    15901 cgctgctcca ggaacgagcg cagccgcggc accaaccgct gggcctcaac cggctcgcag
    15961 gtcagatagg ccacgagacg ccgctgcccc ggctcgtcct cccggctcac gaccaccgcc
    16021 tcccgcacag ccgggtgctc gcgcaacgcc tcttccacct ctcccggctc gatgcggaac
    16081 ccccgcacct tgacctgctc gtcggcccgg cccaggtact ccaggacacc atcggcccgg
    16141 tagcgcgcca ggtcgcccgt ccggtaccaa ccgtccacga accgctcccg cgtcagctcc
    16201 ggacggttcc agtacccccg gctgaccgcc gcgccgccga tccagatctc cccggccact
    16261 cccagcggca cttgctgccc gcgccgatcc cgcagctcca agagggtgtt gcccagcggt
    16321 cggccgagcg gcggcctcgc gagtgcctgg ccgtccaccg gatggctcgc gcagaggatc
    16381 gtgccttcgg tgggaccata gagcacccgc acctcggcac cggggaacac ctccagcagg
    16441 tccgccagca ggtccggtgg caccgcctcg ccgccgacga atacggtccg cagaccgctg
    16501 atccgcccgg cctggctccg gatctcctcg acgatctccc gcatcaggga cggcaccgcg
    16561 tgcagccgcg tcatcgccgg gagagcctcg gtcagccgcc gcacgtccgg caccggccgc
    16621 agcgcgaaca gctccgcccg gccgccggcc agcagcgggt tccacagctc gaacaggaag
    16681 atgtcgaaag agtacgccgc gagcaccggc atccggtccc ggccatccca gtccaggtca
    16741 cgctggatca ccgacaggac gttggccaga ctgccatgat ccaccagaac ggccttgggg
    16801 cggccggtcg tgcccgaggt gaagatcagg taggcggccg attcgggccc cacctccacc
    16861 ctcgggcggc cgtccctccc cgaggccatg gccgcggcga cctgctcccg gtccaccacg
    16921 atgggggtag cgtcaccgaa cacctctcgg agcatcccct ccaggcgcgg gcgcggcgcc
    16981 tccggatcga gcggcacaaa ggcgcctcca gccttccaca cgcccaacat cgcgaccgcg
    17041 atctccaccg accgctccag gcgaagcgcc accggtgctc ccggacgtac acccagtgcg
    17101 atcaagtgat gggccaggcg gttggcccgg cggtcgagct cgcgataact cagctcctca
    17161 cccatgaagg ccaccgccac tgccgacggc accgccgcgg cgcggcgctc gaacaaccgg
    17221 tgcaccggct cggcgggcag cgccgtcgcg gtgtcgttcc actcgcgcga gagctggtgc
    17281 gtttcggcct cgccgagcag agggatctcg cccacgggcc gctccggatc ttccacggcc
    17341 gccgcgagca gcgccggcag atagccggtg aagcgccgcg ccgtggcttc gtcgaagagg
    17401 tcgaggttgt actcgagaaa gccgtggagc gctccgcccc cctcgaagag gtggagcgac
    17461 aggtcgacct tggaggtgcc ggtgccgacc gcctcctccg ccggcgggat caggacaagc
    17521 cctccggtct cgacctcgcg cgaggtgaag ttgtggaagc cgaaggtcac ctggacgagc
    17581 gggttgcggc cgaggtcccg gtccggcgcc agctcttcga ccagcttctc gaagggaaga
    17641 tcctggtggg cgtaggcccc gagagaggtc tcccgcacgc gggccaccag gtcgccgaag
    17701 cccggcgcat cctcgagccg ggtgcgcagg accagggtgt tgacgaagaa gccgatcagc
    17761 tcctcggtct cgcggcggtt gcggccggcg atggccacgc ccacggggat gtcgccgcgg
    17821 ccggagagcc gggagaggag aaccttgaag cctgcgagga ccaccatgga gagcgtggcg
    17881 ccgcggcggc gggcgagcag cgacagccgg cgtaccagcg gcgccgcgag agggaaggcg
    17941 gcgccggcgc cacggaagga ctcgatggcc ggccgcggcc ggtcggtagg cagctccagc
    18001 acctcgggca tgtctgcgag ccggcccctc cagtagtcca gccgtgcgcg cagggcctca
    18061 ccctggagcc agccgcgctg ccagacggcg aagtcggcgt actggaccgg cagcgccggc
    18121 agtggggacc cttgccgccg gtggaaggcg ccatagagcg cggcaaggtc gcggtggagg
    18181 acgcccagcg accagccgtc cgaggcgatg tggtggacgt tgaggaagag ggagtgctcg
    18241 ctctggctca gccgcagcag agcggtgcgg aagaccggcc cgcaggccag gtcgaacggc
    18301 cgccgcccct cggcgagggc gagacgcgcc agttcctccc gccgcaccga ttccgtgagc
    18361 cccccgagat cgatcaccgg aaaacccgga ggcggctcgg cggcgatggc ctgcac
//
LOCUS       DASPJI010000091         5712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_749486, whole genome shotgun sequence.
ACCESSION   DASPJI010000091 DASPJI010000000
VERSION     DASPJI010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749486"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..392)
                     /locus_tag="VEL74_02425"
     CDS             complement(54..392)
                     /locus_tag="VEL74_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61413.1"
                     /translation="MSMHFFTFLAVPTRDAKEFHEAGGAYVNCWIQEEDRERAQESAT
                     DLIQDYGWTVESLEEAGTVTGDDYGLEDEDRQFYEQALMEGEVLVFNTWPRGDDGGEE
                     GEAEEGETWQ"
     gene            531..1469
                     /locus_tag="VEL74_02430"
     CDS             531..1469
                     /locus_tag="VEL74_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HYG61414.1"
                     /translation="MAEASFERRIGDVRRFNRFYTRQIGVLHEGLVRSPYSLTEVRVL
                     WELAHRDRPTAAGIGKELGLDAGYLSRILRGFQQRGLVASEPSETDARQNLLRLTEEG
                     IDAYAVLDARSRDDVASLLSRLSEAEQRRLTEAMSTIEALLGAPSESKASYLLRTHQP
                     GDMGWVVARHGVLYAQEYGWDETFEALIAEIVAKFIRELDPKRERCWIAELDGENVGC
                     VFLVKHPEMPERVAKLRLLLVEPTARGLGIGRRLVDECIRFGRQAGYRKITLWTNSVL
                     DAARHIYEQTGFALVQEEPHHSFGHDLIGQTWELEL"
     gene            complement(1773..2006)
                     /locus_tag="VEL74_02435"
     CDS             complement(1773..2006)
                     /locus_tag="VEL74_02435"
                     /inference="COORDINATES: protein motif:HMM:NF040441.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PF20097 family protein"
                     /protein_id="HYG61415.1"
                     /translation="MDHHLTCPKCSAAMEKGVIVDYTHGAATQAEWAEGEPKYSIWTG
                     LKLRGHERHRIVTYRCTRCGYLESYAPTDSNKG"
     gene            2055..2246
                     /locus_tag="VEL74_02440"
     CDS             2055..2246
                     /locus_tag="VEL74_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61416.1"
                     /translation="MSPRPLGERPERGDRPAFAAAHRSSVLLSSIVRPIAGREPFYQL
                     GTERWRDGYPLLRNGSPQG"
     gene            2438..3955
                     /locus_tag="VEL74_02445"
     CDS             2438..3955
                     /locus_tag="VEL74_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYG61417.1"
                     /translation="MERDAVVVGAGPNGLAAAIALGQAGWSVTVVEARDTVGGAARSA
                     ELTLPGVLHDVCSAIYPLTVGSPFLSQLPLERHGLRWIEPPLQLAHPLDDGTAAVLAR
                     DFETTGESFGDPADAEAWRELFAPLTESWDDLARDFLGPIKLPHHPFKLARFGLMGLR
                     SAASLAESRFRGFRARALFAGIAGHSFLPLEMIPTASYGLVLAASGHAVGWPQPLGGA
                     QKLSDAMASLVREAGGEIRTGQLVESLDELPSARAYLLNITPAQLLRMGGVRLTPGYR
                     RRLERYRYGPGVFKIDWTLSEPIPWRAEACRRAGTVHLGNTFHEVAAAENAAWYGRVP
                     ARPFVLLGQPTLFDSTRGPAGVHTAWAYCHVPHGSTADMTAAIEAQVERFAPGFRDVV
                     VARHTMNSAQLEAYNPNCIGGDINGGAALLTQLFTRPVISLVPYATPDPRVFLCSAST
                     PPSGGVHGMCGYHAAEVVMRRLGWGSGTAGAPRTTAEDSHDLSSAHPPRRAGGQR"
     gene            3952..5532
                     /locus_tag="VEL74_02450"
     CDS             3952..5532
                     /locus_tag="VEL74_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYG61418.1"
                     /translation="MKVLVTGGTGVVGAAAVRALLADGHAVRLFSRHADKDVERWRGT
                     GDIEAREGSIGSPLDIAGAAEGCDAILHIAGIVEERPPEVTFDRVNVGGTQHLLSEAQ
                     RAGVRRFVYVSSLGAERGDSAYHSSKRRAEDLVLQFPGDWLICRPGNVYGPGDEVISL
                     LLQMVRISPVVPVIDAGDQPFQPLYAEDLGAALAIAVSRKELSRQVLLLAGDEVTTTS
                     ALIDRLARITGREAARLPVPSFFAKLGAQAASMIGLRMPVNEDKLTMLIEENVIRPGE
                     VNALTTILGVKPTPLEEGLRRLAVSQPEQLPSGGTGTGPVMRRRYWANLHGIPGTAKD
                     LFTEFRRRFFDVVPEATVREGGEQVGERRLDPGAVLTLSLPMRGHVQVRVVEVTDTSA
                     TLVTLAGHPLAGSNTFRITGVPTGTPSDAPHGGLRFVVESCDRPSNLVDGLAMTPMGT
                     MLKHWTWNNVVEEAAKMFGARPPIDVQMEEHALEGEAERQAIREMEEKVANLAREQEP
                     ALQEEAKARLEEASVGKI"
BASE COUNT          831 a         1992 c         1996 g          893 t
ORIGIN      
        1 agaggttctc ggcacccggg ttttcacgtg gcccgggtgc ccgcggcctc agcttactgc
       61 caagtctccc cctcctcggc ttcgccctcc tcgccgccgt cgtcgccgcg cggccaggtg
      121 ttgaagacga ggacctcgcc ctccatcagt gcctgctcgt agaactggcg gtcctcgtcc
      181 tccaagccgt agtcgtcacc ggtgacggtc cccgcctcct cgagggactc cacggtccat
      241 ccatagtcct ggatcagatc ggtggcgctc tcctgcgccc tctcccggtc ctcctcttga
      301 atccagcagt tgacgtaggc gccgccggcc tcgtggaact ccttcgcgtc gcgagtcgga
      361 acggctagga aagtgaagaa gtgcatgctc ataacgtaga tccctccatg cccgcacaca
      421 cccgcgggtc tcagtatcct gatgcaaacc cacggcccgc gccaactcca tagttgacgc
      481 tgtcaactag ttggtggaca atggcactca tcagggtgga ggaggttggc atggcggagg
      541 cgagcttcga gcgccgcata ggggacgtgc gccgcttcaa ccggttctac acgcgccaga
      601 tcggggtgct gcacgagggg ctggtgagga gcccgtactc attgaccgaa gtgcgcgtgc
      661 tctgggagct ggcgcaccgc gaccggccca cggcggccgg gatcggcaag gagttggggc
      721 tggacgcggg ctatctgagc cggatcctgc gcggcttcca acagcgcggc ctggtcgcca
      781 gcgagccctc cgagaccgac gcccggcaga acctcctgcg gctgacggag gaggggatag
      841 acgcctacgc ggtcctggac gcgcgctccc gggatgacgt cgcgagcctg ctgagccgtc
      901 tctccgaagc cgagcagcgc cgcctgaccg aggccatgtc caccatcgag gcgctgctcg
      961 gcgccccttc cgagagcaag gcttcctacc tgctgcgcac ccaccagccg ggggacatgg
     1021 gctgggtggt cgcccggcac ggcgtcctct acgcgcagga gtacggctgg gacgagacct
     1081 tcgaggcgct gatcgccgag atcgtggcga agttcatccg cgagctcgac ccgaagcgcg
     1141 agcgctgctg gatcgccgag ctggacggcg agaacgtcgg ctgcgtcttc ctggtcaagc
     1201 atccggagat gccggagagg gtggcgaagc tgcggcttct gctggtcgag cccacggcgc
     1261 gcggcctcgg catcggccgg cgcctcgttg acgagtgcat acggttcggc cggcaggcgg
     1321 gttaccgcaa gatcacgctc tggaccaaca gcgtcctcga cgccgcccgg cacatctacg
     1381 agcagaccgg cttcgcgctg gtgcaggagg agccgcacca cagcttcggc cacgacctga
     1441 tcggtcagac ctgggagctg gagctgtgag cgcggctgct tcctgcggcc ggtcctaatt
     1501 cccgctcgag ccggtcgtgc acgccgcggc cggcgggttg accgccacct tcgggtggtt
     1561 cttctgcatg tattggtagt agtgctcgtt ggcgaaggcg tcccgacgca cgagctgcga
     1621 ggctcaccgg tccggaaggt cgttacgccg gtccgggcgg atggagcaga ccgccggacc
     1681 gacgcttcat ccgttcggcg ggttgtgacg ccggtccgga ggaatggagc aaccgaccgg
     1741 accgacgctc catcggttcg gtgggccggc ctttaccctt tgttgctgtc ggtaggagcg
     1801 taggactcca ggtatccgca gcgggtgcag cggtaggtca cgatgcggtg acgctcgtgg
     1861 ccgcggagct tgaggccggt ccagatcgag tacttcggct ccccctcggc ccactccgcc
     1921 tgcgtggccg ctccgtgggt gtaatcgacg atcacgcctt tttccatcgc ggccgagcac
     1981 ttcgggcaag tgaggtggtg gtccataagc cctcgcgcgg catcttacct ctggatggcc
     2041 agacctcgac ggcgatgagc ccgaggcctc tcggcgaacg gcccgaacgg ggcgaccggc
     2101 cggcgtttgc cgccgcccac cggtcgagcg tccttctcag ctccatcgtc cggcccatcg
     2161 ccggacgcga gccgttctac cagctcggca cggagagatg gcgcgacgga tatcccctgc
     2221 tgcgcaatgg ctctccccag ggctgaatgg agatcttcca tcaagattgg gcgtcgggcc
     2281 tgagtctgct cgaacgcgtc gtccgcgatc cgctggccgt cgcgctcgtc ctcgaagccg
     2341 cgggaccgtc ctgcccaaag ggccctggaa ccgaagttgc acaaccggcc ggggaggtgg
     2401 gatggtgagg aagccggagc acgaaacgag gttccccatg gagcgtgacg cggtcgtcgt
     2461 cggtgccggg cccaatggcc tggcggccgc gatcgccctg ggacaggcgg ggtggtcggt
     2521 caccgtcgtc gaggcgcgcg acaccgtcgg cggggcggcg cggtcggcgg agctgacgct
     2581 gccgggggtc ctccatgacg tctgctcggc catctatccc ttgacggtgg ggtcgccgtt
     2641 cctctcccaa ctgcccctcg agcggcacgg cctccggtgg atcgagcccc ctctgcaact
     2701 ggcgcatccg ctcgacgacg gcacggccgc cgtcctcgcc cgggacttcg agaccacggg
     2761 ggagagcttc ggcgacccgg ccgacgcgga ggcatggcgg gagctgttcg ccccactgac
     2821 cgaatcctgg gacgatctgg cgcgggactt cctgggtccc atcaagctgc cgcatcaccc
     2881 gttcaagctc gcccgcttcg gcctgatggg gcttcgctcg gcggccagcc tggcggagag
     2941 ccggttccgc ggcttccggg cccgggcgct cttcgccggt atcgccgggc attcgttcct
     3001 ccccctggag atgatcccga cggcgtcgta cggcctggtc ctcgccgcgt ccggccacgc
     3061 ggtggggtgg ccccagcccc tcggtggcgc gcagaagctc tccgacgcca tggcgagcct
     3121 ggtgcgcgag gccgggggag agatccgcac cggccagttg gtcgagtcac tggacgagct
     3181 gcccagcgcc cgggcctatc tgctcaacat caccccggcg cagctcctgc gcatgggcgg
     3241 cgtccgtctc accccgggct accggcggcg cctcgagcgc taccggtacg ggccgggggt
     3301 gttcaagatc gactggacac tgtccgagcc gatcccgtgg cgggccgagg cctgccgccg
     3361 tgccggcacc gttcacctcg gcaacacgtt ccacgaggtg gcggcggccg agaacgccgc
     3421 ctggtacggc cgcgtgccgg cccggccgtt cgtcctgctc ggccagccga ccctcttcga
     3481 ctcgacccgc ggaccggccg gcgtccacac cgcgtgggcc tattgccacg tgccgcacgg
     3541 ctccacggcc gacatgaccg cggccatcga ggcccaggtg gagcgcttcg cccccgggtt
     3601 ccgcgacgtc gtcgtggcgc gccacaccat gaactcggcg cagctcgagg cgtacaaccc
     3661 caactgcatc ggcggcgaca tcaacggtgg cgccgcgctc ctcacccagc tcttcacccg
     3721 ccccgtgatc agcctcgtcc cctacgcgac gccggacccg cgggtcttcc tgtgctcggc
     3781 gagcacgccc ccctcgggcg gcgtgcacgg catgtgcggc tatcacgcgg cggaggtggt
     3841 gatgcggcgg ctggggtggg ggagcggaac ggccggcgcg ccgaggacga cagccgagga
     3901 ttcgcacgat ctctcttcag cacacccgcc acggcgggca ggaggacaac gatgaaagta
     3961 ctggtgacgg gtggaaccgg ggtggtgggt gcggcggcgg ttcgtgccct gctggcggat
     4021 ggacacgcgg tgcggctctt ctcgcggcac gccgacaagg acgtggagcg ctggcgcggc
     4081 accggcgaca tcgaggcgcg ggaggggagc atcggctcgc cgctggacat cgccggtgct
     4141 gccgaggggt gcgacgccat cctccacatc gccggcatcg tcgaggagag gccgcccgag
     4201 gtcaccttcg accgggtgaa cgtcggcggc acccagcacc tcctcagcga ggcgcagcgg
     4261 gccggcgtcc gccgcttcgt ctatgtctcg tccctgggcg ccgagcgcgg cgactccgcc
     4321 tatcactcct ccaagcgccg ggccgaggac ctcgtcctcc agttccccgg cgactggctg
     4381 atctgccggc ccggcaacgt ctacggcccc ggcgacgagg tgatctcgct ccttcttcag
     4441 atggtgcgga tctcgccggt cgtaccggtc atcgacgccg gggatcagcc gttccagccc
     4501 ctctatgccg aggacctcgg cgcggcgctg gcgatcgccg tgtcccgcaa ggagctgtcg
     4561 cggcaggtgc tgctcctggc gggcgacgag gtcaccacca ccagcgctct catcgaccgc
     4621 ttggcccgga tcaccggcag ggaggcggcg cggctgccgg tgccctcctt cttcgccaaa
     4681 ttgggagccc aggctgcctc gatgatcggc ctgcgcatgc cggtcaacga ggacaagctc
     4741 accatgctca tcgaggagaa cgtgatccgt cccggcgagg tcaatgcgct gaccaccatc
     4801 ctcggggtca agcccacgcc gctggaggag gggctgcgcc ggctcgccgt cagccagccc
     4861 gagcagctcc cgagcggggg cacgggcacc ggcccggtga tgcggcgccg ctattgggcg
     4921 aacctgcacg ggatccccgg cacggccaag gatctgttca ccgagttccg gcgccgcttc
     4981 ttcgacgtcg tccccgaggc cacggtccgt gagggggggg agcaggtggg ggagaggcgg
     5041 ctcgatcccg gagccgtgct caccttgtcg ctcccgatgc gcggccatgt ccaggtgcgg
     5101 gtggtggagg tcacggacac ctcggcgacg ctggtcaccc tggccggaca cccgctcgcc
     5161 ggctccaaca cgttcaggat caccggcgtc ccgaccggta cgccgagcga cgcgccgcac
     5221 ggcgggctgc gcttcgtggt ggagtcgtgc gaccggccct ccaacctggt ggacggcctg
     5281 gccatgaccc ccatgggcac catgctcaag cactggacct ggaacaacgt ggtcgaggag
     5341 gccgcgaaga tgttcggcgc ccggccgccc atcgacgtcc agatggagga gcatgccctg
     5401 gagggggagg cggagcgtca ggccatccgc gagatggagg agaaggtcgc caaccttgcc
     5461 cgtgagcagg agccggcgct ccaggaggag gccaaggccc ggctcgagga ggcctcggtc
     5521 gggaagatct gaggcgcgag ctgaaaccgg agacgccccg acggctgcgg cgatcctcga
     5581 cgagcgcacg gcgtccgtga aatagctccc ggccctttcg gagccggcgt gacccttttt
     5641 agagctgccg cgaccccagg gtcgaggcag gtttgaggag ggtcgaacgg cttgacccca
     5701 gggtcgggcc ag
//
LOCUS       DASPJI010000092         3591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_5135, whole genome shotgun sequence.
ACCESSION   DASPJI010000092 DASPJI010000000
VERSION     DASPJI010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5135"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1940
                     /locus_tag="VEL74_02455"
     CDS             <1..1940
                     /locus_tag="VEL74_02455"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HYG61419.1"
                     /translation="GVTAGKVRLLAAAPRLLQAEIPILEVAAGKDVEDLELVLRRGSV
                     VAGRVLSPGGEPVTGARVEPAAQAEDDVFLPFFQGTVSDAEGRYRLEGVPDGAVRSFA
                     AEAEGFQRAVREVEVRPGENRLDFRLDAGHEISGRVVDAAGAPIAGALVSTSEPGSPF
                     MGAEGGWGKGVRTGADGSFRLAGVATGVHEVRAGKEGYAPGRLQAVEVAGPVQGLELR
                     LGAAGSVHGRLTGLDLAELAGVEVSAQPLSPGALPGYANGQVDYEGRYRVGGLTAGEW
                     RVEVQAPSGLRASGKVTVPAGGDAVLDLEFKDGFTLTGRVLRGGEPVGEAQVVAMATD
                     SDSHGFSSTGPQGAFRIPGLAPGSYQVRVVNHRGLYHQETVEISGDRDLRIEISGQRV
                     SGRVVDATDQSPIVRARVSLEAEAAPGTSRAASSDDQGAFTLTDVTPGVYRIVARVDG
                     YAPASTPVEVREAGVDGVMLALQPTEGLVLEVRGTLGSPPPQVQVAFLDATGRSILQG
                     SHSTGENGRVRLSSAPPGTWQLLVSASGYATIERPVQVPSPTVAIALPRSSTLTVEAP
                     GLPEHAVVTVTSAGGDGGRRFRSLDWQVEDEWHVHGGKAEIAGLPPGRWRVRVATPDG
                     GQAWQAEVATVAGSPSRVVVR"
     gene            2043..2729
                     /locus_tag="VEL74_02460"
     CDS             2043..2729
                     /locus_tag="VEL74_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61420.1"
                     /translation="MAEDSSQDWERLLAAERHLQSLVPGTVLVGGTAAALHAEHRVSF
                     DGDHVLADLAGRFDEVLADLESVAGWRTNRVRRPVLILGSLEGVLTGIRQLRRVRPLE
                     TEEIAGLQVPTLAEMARIKAWLLATRATTRDYLDVVVLLERLGEVGAVKALQPLDEIY
                     PQASGASVLAEVIERLGAAQPVDAAAIDLATYRGLKPPWNGWNHVADRGRHWARRFAT
                     LLLGSAEEAR"
     gene            2726..3025
                     /locus_tag="VEL74_02465"
     CDS             2726..3025
                     /locus_tag="VEL74_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61421.1"
                     /translation="MSRFDLSTSRALWNREGLNLESDEVLAQILDRGELEAWRELYRL
                     AAAPGDEAARLRRRILHLCRTVPLAFPHLFIAAMGALGEPVEPYPSVPPPADEIA"
     gene            3165..>3591
                     /locus_tag="VEL74_02470"
     CDS             3165..>3591
                     /locus_tag="VEL74_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYG61422.1"
                     /translation="MRRIWLALTLALWGAAAIPAHAAPVPIGGRIVDPDGRPVRGASV
                     ELGPVPDAFGWSELRLHGKILPEPVARAASRDDGRFEIAAPAPGMWQVIVAARGFVPR
                     IYYLLPAFEATELPLLEMTRDVPLRVRVLGPQGKPVDGAR"
BASE COUNT          478 a         1260 c         1340 g          513 t
ORIGIN      
        1 agggggtgac ggcgggcaag gtccggctcc tggccgcggc gccgcgcttg ctgcaggcgg
       61 agatccccat cctcgaggtg gcggccggca aggacgtcga ggatctcgag ctggtcctcc
      121 ggcgcggctc ggtggtggcc ggccgcgtcc tgagcccggg cggcgagccc gtgaccgggg
      181 cgcgggtgga gccggccgct caggcggagg atgacgtctt cctgcccttc ttccagggga
      241 ccgtctccga tgccgagggc cggtaccgtc tggagggcgt gcccgacgga gctgtacggt
      301 ccttcgccgc cgaggccgaa ggcttccagc gcgccgtcag ggaggtggag gtccggcccg
      361 gggagaaccg gctggacttc cggctggacg ccggccacga gatctccggc cgggtggtgg
      421 acgccgccgg agcgcccatc gccggagccc tggtgagcac ctccgagccc gggtctccgt
      481 tcatgggggc tgaaggcggc tggggaaagg gggtccgcac cggcgccgac ggctcgttcc
      541 ggctcgccgg cgtggccacc ggcgtccatg aggtccgcgc cggcaaggag ggctacgcgc
      601 cgggccggct ccaggccgtg gaggtcgccg gaccggtcca gggactggag ctgcggctcg
      661 gcgccgccgg gagcgtccac ggccgcctca ccgggctcga cctcgccgag ctggccggcg
      721 tggaggtctc cgcccagccc ctgagccccg gcgccctccc cggctacgcc aatggacagg
      781 tggattatga aggccggtac cgggtcggtg gcctgacggc aggcgaatgg cgggtggagg
      841 tccaggcccc ctccggcctc cgggcttccg gcaaggtgac cgtgccggcc ggcggcgacg
      901 cggtgctcga cctggagttc aaagacggct tcaccctcac cggccgggtc ctgcgcggcg
      961 gcgagccggt cggcgaggcg caggtggtcg ccatggcgac ggacagcgac agccacgggt
     1021 tctcgtccac cggcccccag ggagccttcc gcatccccgg gctggcgccg ggctcctacc
     1081 aggtgcgggt cgtcaaccac cgcggcctgt atcaccagga aacggtggag atctcggggg
     1141 accgcgacct ccggatcgag atctcggggc agcgggtgag cggccgggtg gtggacgcca
     1201 cggaccagtc gcccatcgtc cgcgcccgcg tctccctgga ggcggaggct gctcccggca
     1261 cctcgagggc cgcgagcagc gacgaccaag gggccttcac cctcaccgac gtcacccccg
     1321 gggtctaccg gatcgtcgcc cgggtggacg gctacgctcc ggcctccacc ccggtcgagg
     1381 tgagggaagc cggcgtagac ggcgtgatgc tggcgctcca gcccacggaa gggctcgtcc
     1441 tggaggtgcg cggcaccctg gggagcccgc cgccgcaggt ccaggtggcc ttcctggatg
     1501 ccacgggccg gagcatcctc caaggcagcc actccaccgg cgagaacggc cgggtccgcc
     1561 tctccagcgc gccgccgggg acctggcagc tcctggtgtc cgccagcggc tacgccacga
     1621 tcgagcggcc cgtccaggtc ccctccccca cggtcgccat cgccctcccc cgctcctcca
     1681 ccctgaccgt ggaggcgccc gggttgcccg agcacgccgt ggtcacggtc accagtgccg
     1741 gcggcgatgg cggccgccgc ttccggtcgc tggactggca ggtggaggac gagtggcacg
     1801 tccacggcgg caaggcggag atcgcgggcc tgccccccgg ccgctggcgg gtgcgcgtcg
     1861 ccacgccgga cggcgggcag gcctggcagg ccgaggtggc gacggtcgcg gggagcccct
     1921 cccgggtggt ggtgcggtga cccgggagag cgcgatacgg cggatctgtc tgctcctggc
     1981 cgtggggctg tccaagaagg gccccctccc cttccgccgg attccgaaga tatcctagaa
     2041 ggatggccga ggactcttct caggattggg agcgcctgct cgccgcggaa cgccacctac
     2101 agtctctggt accgggcacg gtcctggtag gtgggaccgc tgccgctctg catgccgagc
     2161 atcgcgtgag cttcgacgga gaccatgtgc tcgccgatct cgcgggtcga ttcgacgagg
     2221 tgctggccga cctcgagagc gtggcagggt ggcggacgaa ccgggttcgc cggccggtcc
     2281 tcatcctcgg gagtctcgag ggtgtcctga ccggcatccg tcagcttcgc cgagtccgtc
     2341 ccttggagac cgaggagatc gctgggttgc aggtgcccac cctggcggag atggcccgca
     2401 tcaaggcctg gctcctggca acccgcgcga cgacgcgcga ctacctcgac gtggtggtgc
     2461 tcttggagcg gctcggagaa gtgggggctg tgaaggccct tcagcctctg gacgagatct
     2521 acccccaagc gagtggagcc tcggttctgg ccgaggtcat cgaacgtctg ggagctgccc
     2581 agcctgtgga cgccgccgct atcgacctcg cgacctatcg cggcctcaag ccaccctgga
     2641 acggctggaa ccacgtcgcg gatcgcgggc gccactgggc gcggcggttt gcgaccctgt
     2701 tgctgggctc agcagaggaa gcccgatgag ccgcttcgat ctctcgacct cgcgggcgct
     2761 ctggaaccga gaagggctga acctggagag cgacgaggtg ctggcgcaga tcctggaccg
     2821 cggggagctc gaggcttggc gcgagcttta ccgtctggcc gcggcacccg gggacgaggc
     2881 cgcgcggctc cggcgccgta tcctgcacct ctgccggacc gtgccgctcg ccttccctca
     2941 cctcttcatc gccgccatgg gggcgctagg cgagccggtc gaaccctatc cgtccgtgcc
     3001 gccaccggcg gacgaaatcg cctgaagcct caaaggggag gcacgccgcc cataaagagg
     3061 gggcacgctc ccactacacg tgggagaggg ggccagaggc gaggcttata gctcacgctg
     3121 caatgacccc cgcacccttt agaatgggca gaacggaggc ttcgatgcga cggatctggc
     3181 tggctttgac cttggcgctg tggggggcgg cggcgatccc tgcacacgcg gcgcccgtcc
     3241 cgatcggtgg gcggatcgtg gaccccgacg gccggcccgt gcgtggcgcg agcgtcgagt
     3301 tggggccggt gccggacgcg ttcggctgga gcgagctgcg gctccatggc aagatcttgc
     3361 cagagccggt ggcgcgggcg gccagccgcg acgacggccg cttcgagatc gcagcgccgg
     3421 cgcccggcat gtggcaggtg atcgtcgccg cccgcggctt cgtcccccgg atctattacc
     3481 tgctcccggc cttcgaggcc accgagctcc ccctcctcga gatgacccgc gacgtgccgc
     3541 tgcgtgtccg cgtgctcggt cctcaaggta agccggtgga cggggcccga g
//
LOCUS       DASPJI010000093         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_807158, whole genome shotgun sequence.
ACCESSION   DASPJI010000093 DASPJI010000000
VERSION     DASPJI010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807158"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VEL74_02475"
     CDS             <1..190
                     /locus_tag="VEL74_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005996842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61423.1"
                     /translation="QTGLAGGDHLHFTLLLQGLPVSPVEWWDPHWIQDRVARKLGPAM
                     KYQERDLPAAQPAAAAAR"
     gene            complement(193..2502)
                     /locus_tag="VEL74_02480"
     CDS             complement(193..2502)
                     /locus_tag="VEL74_02480"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HYG61424.1"
                     /translation="MPITSRLRRPRTFLAVLLATLGGAGMLSAQTGDPATASAAAKPS
                     RQRLQLRIPRLSSPVQIDGVLEEEAWSQALKMDVAFEIDPGRNVPAPVTTVAYLAYDD
                     ERLYAAFRADDPDPGAIRAHLADRDTTFRDDFVGIFFDTFNDPRRGFEVFVNPLGVQM
                     DLSIDETNSDEREDASWDIIWDSAGRIDERGYVVEFAIPFSSLRFQRSEGEQTWGIGA
                     FRSYPRSLRHQISSVPLDPDDGCILCQIPQVTGFAGVTPGRNIELDPTATARRTDVRE
                     DFPRGGISEGSTESDLGMTARWGITPNVILTGAVNPDFSQVEADAAQFDVNTQFALFF
                     PERRPFFLEGADFFATPFSIIHTRTVADPAWGVKLTGKEGRHGFGVFVTEDEITNILL
                     PGSQSSSSASLDLRTTDAALRYRLDLGASSSVGAAMTHRKGTGYSNDLLSVDGRWRPT
                     ATDVLTFQAMSSRTEYPLGLAGELDLPTGRLEGSALRAAYNHGSRNWTWYARYEDIDP
                     ELRADLGFMPQVGTTLWLGGLERTWWAKERSGWYTSLSAGGDWDQTEAPDGTVLEREA
                     EAWLQLRGPLQSLYMIDHGRRERFWHGRTYDEKFTHLEVEVRPSGALFLTLAATVGDT
                     IDFDHDRAGDLLRLRPSIDLNLGRSLRLELDHDLQKVDVAGGELLELNVSQLRAIYQL
                     NLRTFLRAVLQYTDVAYNTELYTAEEVERDNEHLFSQLLFSYKLNPQTVLFVGYSDNQ
                     IGGELEGHPLELTRSDRTLFLKLGYALVM"
BASE COUNT          402 a          905 c         1096 g          437 t
ORIGIN      
        1 ccagaccgga ctcgccggcg gcgaccacct gcacttcacc ctgctcctcc aggggctgcc
       61 ggtgagcccg gtcgagtggt gggacccgca ctggatccag gaccgggtgg ccaggaagct
      121 cgggccggcg atgaagtacc aggagcggga cctgccggcg gcccagccag ccgccgccgc
      181 ggcgcggtag ccctacatca ccagcgcgta tcccagcttc aggaacagcg tccggtcgga
      241 ccgggtgagc tcgagggggt gcccctcgag ctcgccgccg atctggttgt ccgagtagcc
      301 gacgaagagc accgtctgcg ggttcagctt gtacgagaag aggagctggc tgaacaggtg
      361 ctcgttgtcg cgttccacct cctcggcggt atagagctcg gtgttgtagg cgacgtcggt
      421 gtactggagc acggcgcgca ggaaggtccg caggttgagc tggtagatgg cgcggagctg
      481 gctgacgttc agctccagca gctcgccgcc ggcgacgtcc accttctgga ggtcgtggtc
      541 cagctccagg cgcaggctgc gccccaggtt gaggtcgatg ctcgggcgca gccgcagcag
      601 gtcccccgcc cggtcgtggt cgaagtcgat ggtgtccccg accgtcgcgg cgagggtcag
      661 gaagagggcg ccgctggggc ggacctcgac ctccaggtgg gtgaacttct cgtcgtaggt
      721 gcggccgtgc cagaagcgct cgcggcggcc gtggtcgatc atgtagaggg actggagcgg
      781 gccgcggagc tgcagccagg cctcggcctc gcgctccagc accgtgccgt ccggcgcctc
      841 cgtctggtcc cagtcgccgc cggccgagag gctggtgtac cagccgctac gctccttggc
      901 ccaccaggtg cgctccaggc cgccgagcca gagcgtggtc ccgacctgcg gcatgaaccc
      961 caggtcggcc cgcagctcgg ggtcgatgtc ctcgtagcgc gcgtaccagg tccagttgcg
     1021 cgagccgtgg ttgtaggcgg cgcggagcgc cgagccttcg agccgccccg tgggcaggtc
     1081 gagctccccg gcgaggccga gcgggtactc ggtgcgcgag ctcatggcct ggaaggtgag
     1141 gacgtccgtg gccgtcgggc gccagcgccc gtcgacgctc agcagatcgt tcgagtagcc
     1201 ggtccccttg cggtgggtca tggcggcgcc gacgctcgac gacgcgccga ggtcgagccg
     1261 gtagcgcagg gcggcgtcgg tggtccgcag gtcgagcgag gccgagctcg acgactggct
     1321 gccggggagg aggatattgg tgatctcgtc ctcggtcacg aagacgccga agccgtgccg
     1381 cccctccttg ccggtgagct tcacccccca ggccgggtcg gccacggtgc gggtgtggat
     1441 gatgctgaac ggcgtggcga agaagtcggc cccctccagg aagaacggcc gccgctcggg
     1501 gaagaagagg gcgaactggg tattgacgtc gaattgggcg gcgtcggcct cgacctgcga
     1561 gaagtcgggg ttgaccgcgc cggtcaggat gacgttgggg gtgatccccc agcgcgccgt
     1621 catgccgagg tcggactcgg tgctcccctc gctgatcccg ccgcggggga agtcctcgcg
     1681 cacgtcggtg cgccgggcgg tggccgtggg gtcgagctcg atgttgcggc cgggggtgac
     1741 gccggcgaag ccggtcacct gcgggatctg gcagaggatg cagccgtcgt ccgggtcgag
     1801 cggcacggag gagatctggt gccgcaggct gcgcgggtag gagcggaacg ccccgatccc
     1861 ccaggtctgc tccccctcgg agcgctggaa gcgcagggag ctgaacggga tggcgaattc
     1921 caccacgtag ccgcgctcat cgatgcggcc ggccgaatcc cagatgatgt cccaggaggc
     1981 gtcctcgcgc tcgtcggagt tggtctcgtc gatcgacagg tccatctgca cgcccagcgg
     2041 gttgacgaag acctcgaagc cgcgccgcgg gtcgttgaag gtgtcgaaga agatgccgac
     2101 gaagtcgtcg cggaaggtgg tgtcgcggtc ggcgagatgg gcgcggatgg cgccagggtc
     2161 cgggtcgtcg gcgcggaagg cggcgtagag ccgctcgtcg tcgtaggcga ggtaggcgac
     2221 ggtggtgacc ggggccggga cgttgcgtcc cgggtcgatc tcgaaggcca cgtccatctt
     2281 cagcgcctgg ctccaggcct cctcctcgag cacgccgtcg atctggacag gggaggagag
     2341 gcgcgggatg cggagctgca gccgctgccg ggagggcttc gccgccgcgg aggccgtcgc
     2401 cggatcgccg gtctgggccg atagcatgcc ggcccctccg agcgtggcga ggaggacggc
     2461 gagaaaggtc cgcgggcggc ggaggcggct ggtgatgggc atgcgggtgg agtcctccgg
     2521 tgtgtcgaga tcctgtgcgg acctcactac gcagagggag cctccagagt tgcgtcgccc
     2581 gcggcggatg ggggaggccg aggggcatac ccgctggacc tcgggcgcag ggggtggtgg
     2641 tcccctctac gcgttgacat gtgggcgcga gagggtggtg gtgcctcccc ccctgggcgg
     2701 gtgggcggcg gtagggagcg ggggaggtca ggaggggggc agcttcttct tcttcttctt
     2761 cttcttctct tcgccacaaa gctgagatcg gatccctccg cccgcccaaa aagcaacccc
     2821 cctcctcgat cctcccccac
//
LOCUS       DASPJI010000094         3635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_408260, whole genome shotgun sequence.
ACCESSION   DASPJI010000094 DASPJI010000000
VERSION     DASPJI010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408260"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1578
                     /locus_tag="VEL74_02485"
     CDS             <1..1578
                     /locus_tag="VEL74_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61425.1"
                     /translation="SQGNPPHAASALVLRYALAAEASLTSIVAPEQGFVTVRFFRGEQ
                     LVHEDAGTLVGLDGSGFMRVRHVAKVATTRAEIVARHRWWKVEERESRLPHLLRACFL
                     FQADLDAHAQQEATHAAWSDFWSDLLGAGGAATGGQLLEPAQRYTLEARVRWRHRDPS
                     TGDETAPSASKVTLFPFVTEDAEAALARRLRPRTRDFLAAGDNWDVETVPFDGEEAVY
                     TERPLHLTFRDGRFDAYYARFGRRVVLRIVNERGEDLFDRLEYLATHATELPEYQQTW
                     YRFVTEGCAPEGASVLWQAGFARFTTLLETNHRYEAALVPVEDTAGAPFDPETVDWND
                     HTPLHTFRFRTSRWPNLTAHVAAHEVLDEVASTAGVSDLAALYTALGSPGPERRFDDQ
                     ALDEALAALGLPPRPPAAVPEVVRVWRATEDGHEIFAVLVDGPEPLVRDAASHFFLRD
                     EASGPQPIFMLGSRSGSRLLLFFHSGVGFDATTASALQLEVIHPFVGLNGQPATEQAT
                     LAIPVPARPPFFDEEAP"
     gene            1575..>3635
                     /locus_tag="VEL74_02490"
     CDS             1575..>3635
                     /locus_tag="VEL74_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61426.1"
                     /translation="MIIDPNLLQLVGLGIQGDPYLLDGRHLRWFFGRMLGFPRSGFRL
                     RRRRNARDFEGLVDFIRRQSLTQAALTQPLTLGVRVSKHKGLVFQPAFPNDGPALRVD
                     EQPVWLDFENGYPPVPEAADPAAYVMLVFARRSSSGVVEATGWYDTRGAAVPRYRSIQ
                     SAAAGSGGGKPGTSPWVYDSIVLHGGLLDRVEITGRDAVLVEVRWLPTAHYANAPDWE
                     EIGLFYLPFTGEPAIYPDWSPNDGASIAKQRLDEAPPQARAPWDEPNHPPPPLDPATA
                     AEIWADLMARHLDGAAFDTTHEAMKLFLEGELVQIVPQALVEFEVQLEPESPVEGEEP
                     AQFKVRPFDTLYAGAVDPARARLLGLLTSDFTDPGDAWDYTIDADYPELWIRWMTDPE
                     GAAAEAAALRKKFGSKPFPWQDVELVPRQRVLSMATCIEQGKVPLPAPPPDLKVDLVP
                     RPLGEPVQADAALSWLATDRNLFEAPDEVRIAFAVRRVVEGEDVAMHPLDPDLLEQGR
                     RAPVPHLPTAESVERYAGRCRIMDTTLERYTLHEWRVSGMDLFGRFSPFATVQEKVVD
                     LIAPPAPPAVRAVLEGDGPSWKLVAEWEWPAEAANLAPDFSRFEVHLRQGAVSPADAK
                     LPGTWGRFENVVNATTLPLSVSLAGSVNPPGAGLTASATVTLDGTTRRFRLEASPVTR
                     AFDAK"
BASE COUNT          484 a         1422 c         1194 g          535 t
ORIGIN      
        1 tcgcagggga acccgcccca cgccgccagc gcgctcgtcc tgcgctacgc gctggccgcg
       61 gaggcctccc tcacttcgat cgtggcgccc gagcagggct tcgtcaccgt gcgcttcttc
      121 cgcggtgagc agctcgtgca cgaggatgcc ggcaccctgg tgggcctgga cggatccggc
      181 ttcatgcgcg tccggcacgt cgcgaaggtg gccaccacgc gggccgagat cgtggcccgg
      241 catcggtggt ggaaggtgga ggagcgtgag tcccggttgc cgcacctcct cagagcctgc
      301 ttcctcttcc aggcggatct cgacgcccac gcgcagcagg aggccaccca cgccgcctgg
      361 tctgatttct ggtccgatct cctgggagcc ggcggcgccg ccaccggcgg ccagctcctg
      421 gagccggcgc agcgctacac gctggaggcc agggtgcgct ggcggcaccg cgacccctcc
      481 accggcgacg agactgcgcc gtccgcttcc aaggtgacgc tcttcccgtt cgtcaccgag
      541 gacgccgagg ccgccctcgc gcgccgcctc cggccgcgta cccgcgactt cctggccgcc
      601 ggcgacaact gggacgtgga gaccgtgccc ttcgacggcg aggaggcggt ctataccgag
      661 cgcccgctgc acctcacctt ccgcgacggc cgcttcgacg cctactacgc ccgcttcggc
      721 cgccgcgtgg tgctccgcat cgtcaacgag cgtggggagg accttttcga ccggctggag
      781 tacctggcca cccacgccac cgagctcccc gaatatcagc agacctggta ccggttcgtc
      841 accgaggggt gcgcgccgga aggcgcctcg gtgctctggc aggcgggctt cgcccgcttc
      901 accaccctgc tcgagacgaa ccatcgctac gaggccgcgc tcgtgcccgt cgaagacaca
      961 gcgggggcgc cgttcgatcc cgagacggtg gactggaacg accacacgcc gctccacacc
     1021 ttccgtttcc gcacctcgcg ctggccgaac ctgacggcac acgtcgccgc gcacgaggtc
     1081 ctggatgaag tggcgtcgac cgccggcgtc tccgacctcg cggccctgta taccgcactc
     1141 ggctcgcccg gcccggagcg ccgtttcgac gaccaagcgc tggacgaggc cctggccgct
     1201 ctcggcctgc cgccgcgccc gcccgccgcc gtcccagagg tggtgcgggt gtggcgtgcc
     1261 accgaagacg gccacgagat cttcgcggtc ctggtcgacg gccccgagcc gctggtacgc
     1321 gacgccgcct cgcacttctt cctgcgcgac gaggcgagcg gcccccagcc catcttcatg
     1381 ctcggctcgc gctcggggtc gcgcctgctc ctgttcttcc actcgggcgt gggcttcgac
     1441 gccaccacgg cctcggcgct ccagctcgag gtcatccacc ccttcgtagg tctgaacggc
     1501 cagccggcca ccgagcaggc gacgctcgcc atcccggttc cggcgcgccc gccgttcttc
     1561 gatgaggagg caccatgatc atcgacccca acctcctcca gctcgtaggc ctcggcatcc
     1621 agggcgatcc gtacctgctc gacggccgtc acctgcgctg gttcttcggc cgcatgctgg
     1681 gcttcccgcg ctcgggcttc cggctgcgcc ggcgccgcaa cgcccgcgat ttcgaggggc
     1741 tcgtcgactt catccgtcgc cagtccctca cccaggccgc cctcacccag ccgctcacgc
     1801 tgggggtgcg cgtcagcaag cacaaagggc tggtcttcca gcccgccttc ccgaacgacg
     1861 gcccggccct gcgcgtcgac gagcagccgg tctggctcga cttcgagaac ggctacccgc
     1921 ccgtgcccga ggccgccgat ccggccgcct acgtcatgct cgtcttcgcg cgccgctcgt
     1981 cgtccggggt ggtcgaggcc accggctggt acgacacgcg cggcgccgcc gtgccccgct
     2041 accgctcgat tcagagcgcg gccgccggtt ctggtggagg caagcccggc acgagcccgt
     2101 gggtctacga ctccatcgtg ctgcacggcg gcctgctcga ccgcgtggag atcaccggcc
     2161 gggacgcggt gctcgtggag gtccgctggc tccccacggc ccactatgcc aacgcccccg
     2221 actgggagga gatcggcctc ttctatctcc ccttcaccgg cgagcccgcg atctaccccg
     2281 actggtcgcc gaacgacggc gcgtcgatcg ccaagcagcg cctcgacgag gcgccgccgc
     2341 aggcccgcgc gccgtgggac gagcccaacc atccgccgcc gccgctcgac cccgcgaccg
     2401 cggccgagat ctgggccgac ctcatggcgc gccacctgga cggcgccgcc ttcgacacca
     2461 cccacgaggc gatgaagctg ttcctcgaag gcgagctcgt ccagatcgtg ccgcaggcgc
     2521 tggtcgaatt cgaggtccag ctcgagcccg agtcgccggt cgagggggag gagccggccc
     2581 agttcaaagt gcgcccgttc gacaccctct atgccggcgc cgtcgacccc gccagggccc
     2641 ggctcctcgg tctgctgacc agcgacttca cagatcccgg cgacgcctgg gactacacca
     2701 tcgacgccga ctaccccgag ctctggatcc gctggatgac cgatcccgag ggcgccgccg
     2761 ccgaggccgc cgccctgcgc aagaagttcg gcagcaagcc cttcccgtgg caggacgtcg
     2821 agctcgtccc tcgccaacgg gtcctttcca tggccacctg catcgagcag ggcaaggtgc
     2881 cgctccccgc gccgccgccc gatctgaagg tcgacctcgt gccgcgtcct ctcggagagc
     2941 cggtccaggc cgacgccgcg ctgagctggc tggccacgga ccgcaacctc ttcgaggcgc
     3001 ccgacgaggt gcggatcgcc ttcgccgtgc gccgggtagt ggagggggag gacgtggcga
     3061 tgcacccgct cgatcccgac ctcctcgagc aggggcggcg cgcgccggtg ccgcatctcc
     3121 ccaccgccga gtccgtggag cgctacgcgg gccgctgccg catcatggac acgacgctcg
     3181 agcgctacac cctgcacgaa tggcgggtga gcggcatgga cctgttcggt cggttcagcc
     3241 ccttcgccac cgtgcaggag aaggtggtgg acctcatcgc cccgccggcc ccgccggcgg
     3301 tccgcgccgt cttggaaggc gacggcccga gttggaagct ggtggccgag tgggagtggc
     3361 ccgccgaggc ggccaacctg gcgcccgact tctcgcgttt cgaggtccat ctgcgccagg
     3421 gagcggtgtc gcccgccgac gccaagctcc ccggcacctg gggccgcttc gagaacgtgg
     3481 tcaacgcgac cacgctcccg ctctcggtct cgctggcggg atcggtcaac ccgccgggcg
     3541 ccgggttgac cgccagcgcc acggtcactc tcgacgggac cacccggcgc ttccgtctcg
     3601 aggcctcgcc ggtgacgcgg gccttcgacg ccaag
//
LOCUS       DASPJI010000095        16353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_577521, whole genome shotgun sequence.
ACCESSION   DASPJI010000095 DASPJI010000000
VERSION     DASPJI010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16353)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16353
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577521"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VEL74_02495"
     CDS             <1..898
                     /locus_tag="VEL74_02495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYG61427.1"
                     /translation="YLFIAREPRGLAALAAAREVQRAAGFDEAACVDADEVRRLNPAV
                     SVDGVLGGSFCPADGFLRPLEVLRGYCEGARRLGVRFRFGEACTGFRLENDRIAAVRT
                     PGGEIATRCAVNAAGAWASHLGRLVGVDVPVVPLRRQVALTEPGHGLPDTMPMTIFAD
                     DGFHLRVRDDRVLLLWPDHPNGPDPFDTSVDARWLAEVVRRTRERVPGLSRVAIDEGG
                     CWAGLYEMSPDGHALLGAAPGREGLFLANGSSGHGVMHSPALGHLLAEIILDGAASTI
                     DTAPLRPGRFAEGRPNRAPELL"
     gene            895..1464
                     /locus_tag="VEL74_02500"
     CDS             895..1464
                     /locus_tag="VEL74_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61428.1"
                     /translation="MKSQEIILIRHGRSAHVETGWIDLQGFHRWREAYEAAGILPTEV
                     PPDAVRQMARRAGVIAASVAPRAIESARLLAEGREVITSPLLVERVPVPPSLRLRLPL
                     AGWALAYGIQWLFRALTSRPHAPEAEVERAREAADWLAELARQHGAVAVVTHASFRTL
                     LASTLMARGWKSAGARRRSHNWSAWALRL"
     gene            complement(1461..2492)
                     /locus_tag="VEL74_02505"
     CDS             complement(1461..2492)
                     /locus_tag="VEL74_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HYG61429.1"
                     /translation="MKLLFLGFGDVGRRLAEILADRGSYPGLAGLDVSVVGIITGSRG
                     AAVNPAGLDLWRALGEINRMTGPGRFGEGYPDASRMTSHEALSELDYDVAIEMTPLDV
                     AGQGAAAISHVRAALERGRHVISCNKGPLAWAWRPLRDLARQKGCAFLFESTVMDGVP
                     AFNLAQRCLMGNSLLRIEGILNSTTNFVLSEMERGLYLEEAVAAAQRIGVTETDPADD
                     IDGWDAAVKLSALANVLLDADLPPEAIERESLSSLSPGRVREALERGHRLKVICEAFR
                     ERGRVTGRVRLRELPLTDCFALVAGTGSILRLTTDILGTLVVAEENPDLSTTAYGVIS
                     DLFRLREGC"
     gene            2568..3365
                     /locus_tag="VEL74_02510"
     CDS             2568..3365
                     /locus_tag="VEL74_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61430.1"
                     /translation="MSGDEVFVLVIAIVLGSLLWSHWLWVAASVAPRLSKPRDRRPLV
                     FYPMAGAVLLFGVLKGFASFDVRDSWVYLFFYMAMGAAWVAVGLRFLGLFGLSGRDDV
                     VERGNRAAGQAIGGGILGITLCFAGANIGDGPGWWVVVFSAGLSTVTFFVLWVILEKL
                     THLADTVTIDRDPSAGVRLAGFFTGTGLILGRAVAGDWVSAGATVRDFAVTAWPALVL
                     LALAAFLERRLRPSREQPFAPAASGLPPAAVYVGLAVLWLVRLRWWS"
     gene            3353..4657
                     /locus_tag="VEL74_02515"
     CDS             3353..4657
                     /locus_tag="VEL74_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HYG61431.1"
                     /translation="MVELTSPFRPVRPVEPDAFAALRRRAIFECCKWDPQVEDVATLA
                     PFPLVMRREAWNEIAALSVALAAETAGAEEELARRPELHGSLGLPRAVRRALAGNDSP
                     CRGAARVVRFDFHWTTGGWRISEANTDVPGGFNEASGFTRLMASEYAGTEVTARAAGD
                     PAGALADAVAAAAPAGSAIALVHATAYTDDRQVMTFLGRELAARGRETVLAAPDHLRW
                     SDGLARVESAWFRGEVGAMIRFFPGEWLPNLPRSCGWKHFFRGGRTPVSNPAAALLTQ
                     SKRFPLVWDRLERDLPTWRRLLPETRDPRDVPRRERRAGEGWVLKPALGRVGDGIGLA
                     GVTPGGALTKIRREAWWFPSWWAAQRRFEAVPHRFGEQDVYPCVGVYTVDGRVAGAYG
                     RVARLPLVDHLAQDAAVLVEESQAESPRQGAAKEIHERAAAL"
     gene            4635..5414
                     /locus_tag="VEL74_02520"
     CDS             4635..5414
                     /locus_tag="VEL74_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61432.1"
                     /translation="MNAQQLYDIWAPAGSVWSQWAKPALFAGFGSPAPSAAPVPPDLP
                     PAGAGSGGESPVLAATNDQRTAYVVDLPGPRSVEVGADLARAGYRPVPLFNTAFHPAA
                     VVSVGAILERLAARAGELEARGLPPEAPPAFLLDSRRLNPGLVQTPGQFDNRWATFPQ
                     DFPSASFLLSRGIQRVVLVHDGGEPDSLDDLAHVLLRWQRAGIEILRQDTSAGASPQP
                     FEVRKPLAFRSLLYRALTAAGLRRNSAGGFGAIIPLATSSG"
     gene            5603..6985
                     /locus_tag="VEL74_02525"
     CDS             5603..6985
                     /locus_tag="VEL74_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61433.1"
                     /translation="MASLYQRVAAHAIRLRLRSRGRNRARRTNLLIQEEVLAAGSSIA
                     TPLETIHIPRDTVLVFADDDPEANWAHPCRYLLYDAADGELYQQVPASFPPYLHQPVP
                     NLQLLRSEFPRGVRWRPSRLPWQPPVLLVPGNRYAILFCGVANYRHVNDLEFLYRTLR
                     QTYGYPTKHIRVLEGDGTLGYTPTPGLDPPPGDPWPGDVSAYQMKVDGPGTRAALASA
                     LNALRRRLKPNDSLLLHTVGHGYTSGDESFLVTHAGPPCAAGDLAERLAQLPPFACLT
                     VMMSQSGAGGFCAPLLAASPARRTTFAAACGPLGSSYGYRDFGYFARDWTAAMRGADP
                     YGAPLPIDADTNGDGRVVAKEAFVFANRVRYEEDSPRYEETPGSRGGCSLKPGWRPAW
                     WDDLWKELLLPWWKRLPPEVFYGRLYDELIPAAGNLREIRDAGLEGWVTVHGRRLRQI
                     VTEVMSRPVE"
     gene            complement(6989..8263)
                     /locus_tag="VEL74_02530"
     CDS             complement(6989..8263)
                     /locus_tag="VEL74_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HYG61434.1"
                     /translation="MRLRRRRFRHFRPILRPHVGRRLPRELRARRVGRLAAKLPQGLL
                     DPGFELLLERIDGAPVLGGNQVEVFFRGADAFRSMGEAGLAAQREILLESYIFKDDAT
                     GRRFLEGMVAAVKRGVTVRVLADALGSFETKTAFWQEMERQGMEVRLFNPLFKKLWYQ
                     PFRDHRKILVVDREVAFTGGMNIGEEYGSFARRPAATWRDTHVRVAGPTAWEMAVVFS
                     EGWIEADGAPFDIDPLSAEEAEAPGARILVLDSRPNRGQAESASALAAIAGAARRNLW
                     ITNAYFAPGHRAVRLLGDAVQRGVDVRLLLPGKTDVQVVRRAGHGYFTDLLERGVRIF
                     EYQSAVLHAKSLVADGHVSVVGSTNLDFRSFLFNSECDLVILDPGTAAVMEDAFRRDM
                     EESEEILLEDWRQRPLTRQIGDRMARWLSPVL"
     gene            8320..9696
                     /locus_tag="VEL74_02535"
     CDS             8320..9696
                     /locus_tag="VEL74_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYG61435.1"
                     /translation="MRAIATRPSLILLVLFLTAQALSAQVAQLSDADLTTRAERFVDL
                     LAQGGFEEAAKGFDNTMKAAAPPAKLAEIWKALQAQLGPFQKRTATRVEPLGGYRSAF
                     VTSRFEKDAVNLQVVFDGEGRIAGFFVKPAPAAAGAAPAPGTTPALPAYIKKDSFQEK
                     EVEVGAGEWAVPGTLSIPTGKGPFPAVVLVHGSGPNDRDETLGPNKPFRDLAWGLASK
                     GIAVLRYEKRTRFHGDKLASLPKPTVKEETVDDAVAAAQLLRRTEGIDPRRVFVLGHS
                     LGGMLIPRIGARDGEIAGLVILAGLTRPMEETIVQQLSYIASLDGTVTDEEKKRLDEF
                     QEQAAKVKGLTPESTESLMGIPASYWLDLRGYNPPAAAARLRQPLLILQGERDYQVTM
                     DDFQAWKKGLAEKKNVTFKSYPNLNHLFMEGEGKATPSEYERPGHVAAVVIDDIAEWV
                     GKAGTTAK"
     gene            9734..10330
                     /locus_tag="VEL74_02540"
                     /pseudo
     CDS             9734..10330
                     /locus_tag="VEL74_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
     gene            10481..11500
                     /locus_tag="VEL74_02545"
                     /pseudo
     CDS             10481..11500
                     /locus_tag="VEL74_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005482533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
     gene            complement(11559..13115)
                     /locus_tag="VEL74_02550"
     CDS             complement(11559..13115)
                     /locus_tag="VEL74_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HYG61436.1"
                     /translation="MPSQAAPRQPLLQRSLALIERVGNALPHPGTLFALMALAVVLLS
                     GLFSRLDLMVIHPGTGEEIRPVNLLSIAGLHRILTSMVSNFTGFAPLGTVLVAMLGIG
                     IAEASGVIGTALRLLVMSAPRRLLTVVIVFAGVLSNTASEVGYVLLVPLSAIIFLAAG
                     RHPLAGLAAAFAGVSGGYSANLLLGTIDPLLSGLSEEAAHIVDPAYEVNATANFYFMF
                     VSTFVVTAAGTWVTEKIVAPRLGEYRGGEKPEALNRLTPEEKRGLWFTLAALAAVAVI
                     LLLGTIPAGGFLRDPETGSLLRSPFMTGIVSLIFFIFAIAGLAYGFGARTFKSDADVM
                     KGMGKAMETLGLYLVLVFFAAQFVAFFNWTNLGLILAVEGAALLKASGLGPIPLMLVF
                     ILFTAFINLFMGSASAKWAIMAPVFVPMFMLLGFTPELTQAAYRVGDSTTNFVSPMMT
                     YFALIVAFVARYDPKAGIGTVISTMLPYTVVFLLAWALLLAVWMLFDLPMGPGAGLYL
                     PEPQGAASPE"
     gene            complement(13188..13847)
                     /locus_tag="VEL74_02555"
     CDS             complement(13188..13847)
                     /locus_tag="VEL74_02555"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HYG61437.1"
                     /translation="MKIQGEHTFDAPRELVWQALLDPAVLARTLPGCERLEKVNEDEL
                     RGVLNIQVGPVRGQFQGTLRLSDLVPLESYHMRLEGSGPAGFMNGEGDLRLADAADGS
                     GGTVLRYDLDTQVGGRVAGVGQRVLDSSARSVTKQGLEGLARELQALKAAAVPADVGA
                     PAAAAPPPAPVPTQAEFAARVAGDIARDLVPPARRPWLLGGTLLGLVVIVMLLARACG
                     G"
     gene            complement(13844..16192)
                     /locus_tag="VEL74_02560"
     CDS             complement(13844..16192)
                     /locus_tag="VEL74_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HYG61438.1"
                     /translation="MIGQRLKRNEDPRLLTGRALFVDDLNLPGMAHVAFLRSPHAHAR
                     IRGIDVSAAREMEGVVAVYTAADLGDYWQPGPLLVPPPPVEGMVFHQRTQVPLAKDKV
                     RHLGEPVVLVVAESRYIAEDAVERIFVDYEPLPAVVDLEKALSPNAALLHEDVGSNVA
                     AHVVQKKGDYEAARAQADLVVRRRFSYDRGASAAIENRGVVAQWDARAQRLSIWDTTQ
                     APIPVRNGLAAMLGLSEHQVRLVAPFIGGGFGPKIMMFYPEEVLIPWLALRLERPVKW
                     IEDRAENFVGTTQERGQIHDAEIALTRDGRILGVKDSFLHDTGAYDPYGLTVPLNSQC
                     TLMGPYRVPAYASEFRAVFTSKTIVTPYRGAGRQHGVFVIERLMDIAARELGIDGMEL
                     RRRNFLRPDEFPHDHVIIYQDFAPLYYDSGNYAPVLAQTLERIGYERFLAEEQPRLRA
                     EGKAVGIGVVSYVEGTGIGPFEGARVQVQTSGKVTVVTGVGTQGQGHFTVLAQIAAQQ
                     LGVDTDQVDVVTGDSDQFHWGTGTFASRGAVVAGNATNEAAKAVREKTIGLAAQHLGV
                     AEDRIELAEGKARVKDDHGRHVSLGELAKLANPMRGAVKPGTEPGLEATRYFGPFRGA
                     TASGCHACILEVDPETMQVEIKKYVVVHDCGRVLNPLIVDGQVHGGVAQGIGNAYFEQ
                     LHYDDNGQLLNASFMDFLLPTALDVPRVEVGHEETVSPLNELGTKGAGEAGAIPVGAL
                     FAQAVEDALAIPGFEILEIPLSPNRLWELSEEARHAQIRRAS"
BASE COUNT         2356 a         5835 c         5778 g         2384 t
ORIGIN      
        1 ctacctcttc atcgcgcggg agccgcgggg gctcgcggct ctggccgccg cacgcgaggt
       61 ccagcgcgcc gcgggcttcg acgaggccgc ttgcgtcgat gccgacgagg tccgccggct
      121 taaccccgcg gtttccgtgg acggggtcct cgggggctcg ttctgtccgg ccgacggctt
      181 cctgcgtccg ttggaagtac tgcgcggcta ttgcgagggc gcccggcgcc tgggggtgcg
      241 cttccggttc ggagaggcgt gcacgggctt ccggttggaa aacgacagga tcgccgcggt
      301 acggacgccc ggcggcgaga tcgcgacgcg gtgcgctgtg aacgcagcgg gcgcctgggc
      361 ctcacacctc gggcgcctcg tgggtgtgga cgtgccggtc gtgccgctcc gccggcaggt
      421 ggcgctcacc gagccggggc acgggctccc ggacaccatg ccgatgacca tcttcgccga
      481 tgacggtttc catctgcggg tccgcgacga tcgtgtgctg ctgctctggc cggatcaccc
      541 gaacggcccc gatccgttcg acaccagcgt cgacgcgcga tggctggcgg aggtggtccg
      601 gcggacgcgc gagcgggttc ccgggctctc ccgggtagcc atcgacgagg ggggctgctg
      661 ggccgggctc tacgagatgt ccccggacgg gcacgcgctc ctcggcgccg cgcccgggag
      721 ggaggggctg ttcctcgcca acggctcgtc gggtcacggc gtcatgcatt cccccgccct
      781 cggccacctg ctcgccgaga tcatcctgga cggcgcggcg agcacgatcg acaccgcgcc
      841 gctgcgcccc ggccgcttcg ccgagggaag gccgaaccgg gcgcccgagc tgctgtgaag
      901 agccaggaga tcatcctcat ccgtcacggc cggtcggcgc acgtcgagac cggctggatc
      961 gacctccagg gattccaccg ctggcgcgag gcctacgagg cggcggggat cctgccgacc
     1021 gaggtgccgc cggacgcggt gcggcagatg gcccgccgcg cgggcgtcat cgccgccagc
     1081 gtcgcgccgc gggcgatcga atccgcgcgc ctgctggcgg aggggaggga ggtgatcacc
     1141 tcgccgctgc tcgtcgagcg cgtgcccgtc cccccttcac tgcgcctccg tctgccgctg
     1201 gccgggtggg cgctggcgta cgggatccag tggctcttcc gggccttgac ctcgcggcct
     1261 cacgctccgg aagccgaggt cgagagagcg cgcgaggccg cggactggct ggccgagctg
     1321 gcgcgccagc acggagcggt ggccgtcgtc acccacgcgt cgttccggac gctcctggcg
     1381 agcaccttga tggcccgcgg ctggaagagc gccggggcgc gccggcggtc ccacaactgg
     1441 agcgcctggg cgttgcggct ctaacacccc tcccgcagcc ggaacaggtc cgagatcacc
     1501 ccgtaggctg tggtggagag gtccggattc tcttcggcga cgacgagcgt gccgaggatg
     1561 tccgtggtga ggcggaggat ggagccggtc cccgccacca gggcgaagca gtcggtgagg
     1621 gggagctccc gcagccgcac gcggccggtc acccgtcccc tctcgcggaa ggcctcgcag
     1681 atcaccttga ggcgatggcc gcgctccagg gcctcccgca cgcggccggg ggagagcgac
     1741 gagaggctct cccgctcgat ggcctcgggg ggcaggtcgg cgtccagcag gacgttggcc
     1801 agggccgaga gcttcaccgc cgcgtcccag ccgtcgatgt cgtccgccgg gtcggtctcc
     1861 gtcaccccga tgcgctgcgc cgcggcgacc gcctcctcca ggtagaggcc gcgctccatc
     1921 tcgctgagga cgaagttggt ggtggagttg aggattccct cgatccgcag caggctgttc
     1981 cccatgaggc agcgctgcgc caggttgaac gccggcacgc cgtccatcac cgtgctctcg
     2041 aacaggaagg cgcagccttt ctgccgggcg aggtcccgca acgggcgcca ggcccaggcc
     2101 agggggccct tgttacagct gatgacgtgg cggccccgct ccagcgcggc gcgcacgtgg
     2161 gagatcgccg ccgctccctg gccggcgacg tccagcggcg tcatctcgat ggccacgtca
     2221 tagtccagct cgctcagggc ctcgtggctg gtcatccgcg aggcgtcggg ataaccctcg
     2281 ccgaagcggc cgggaccggt catccggttg atctcgccga gggcccgcca gaggtccagg
     2341 ccggcggggt tgaccgccgc cccccgtgat cccgtgatga tgccgacgac cgacacgtcc
     2401 aggccggcga ggccgggata ggagccgcgg tccgcgagga tctccgcgag acgccggccg
     2461 acgtcaccaa agccgaggaa gaggagcttc atggaggata agtctatccc gcccggaggc
     2521 ctgggctatg attccggtct tcgagttcgt gagggaggag ccgctccatg tcgggagacg
     2581 aagtcttcgt cctggtcatc gccatcgtgc tcgggtcgct cctctggagc cactggctct
     2641 gggtggccgc gtccgtggcg ccgcggctgt cgaagccccg cgaccgccgg ccgctcgtct
     2701 tctacccgat ggccggcgcc gtcctcctgt tcggggtgct caagggcttc gcctccttcg
     2761 acgtccgcga ctcgtgggtc tacctgttct tctacatggc gatgggcgcg gcctgggtgg
     2821 cggtggggct ccggttcctc gggctgttcg gcctgagcgg gcgggacgac gtggtcgagc
     2881 gcggcaaccg ggccgcgggg caggcgatcg gcggcggcat cctggggatc accctctgct
     2941 tcgccggcgc caacatcggc gacggcccgg gctggtgggt ggtggtgttc tccgccggcc
     3001 tctcgaccgt gactttcttc gtcctctggg tgatcctgga gaagctcacc cacctggccg
     3061 acacggtgac catcgaccgt gacccgtcgg cgggggtgag gctggccggt ttcttcaccg
     3121 gcacgggact gatcctgggg cgggcggtgg ccggcgactg ggtatccgcc ggggcgacgg
     3181 tgcgcgactt cgcggtcacc gcctggccgg ccctggtcct gctggcgctg gccgccttcc
     3241 tcgagcggcg gctccgcccc tcccgtgagc aacccttcgc ccccgcggcc tcgggcctgc
     3301 cgccggccgc ggtctacgtc ggcctcgccg tcctctggct ggtacggctc cggtggtgga
     3361 gctgacctcc cccttccggc cggtgcgacc ggtcgagccg gacgccttcg ccgcgctccg
     3421 gcgccgcgcc atcttcgagt gctgcaagtg ggacccgcag gtggaggacg tggccacgct
     3481 ggcgccgttc cccctggtga tgcggcgcga ggcctggaac gagatcgccg ccctctccgt
     3541 cgccctggcc gccgagaccg ccggcgccga ggaggagctg gcacggcggc cggagctcca
     3601 cgggagcctc ggtctgcccc gggcggtccg ccgggccctg gccggcaacg actctccgtg
     3661 ccggggcgcc gcccgggtgg tgcggttcga tttccactgg acgaccggcg gctggcggat
     3721 ctccgaggcc aacaccgacg ttcccggcgg cttcaacgag gcctcgggct tcacccgcct
     3781 catggcctcg gagtacgcag gaaccgaggt gacggcccgc gccgcgggcg acccggccgg
     3841 ggcgctggcg gatgccgtgg ccgccgcggc gccggccgga tcggccatcg cgctggtgca
     3901 cgccacggcc tacaccgacg accggcaggt catgaccttc ctcggccgcg agctcgccgc
     3961 ccgcggccgg gagacggtgc tcgcggctcc cgaccacctg cgatggagcg acggcctggc
     4021 ccgggtggag agcgcctggt tccgtggcga ggtgggcgcc atgatccgct tcttccccgg
     4081 cgagtggctg ccgaacctgc cgcggtcctg tggttggaaa cattttttcc gtggcggccg
     4141 gaccccggtg agcaatcccg cggcggccct gctcacccag agcaagcgct tccccctggt
     4201 gtgggaccgc ctggaacggg acctccccac ctggcggcgg ctgctgccgg agacgcggga
     4261 cccgcgggac gtcccccgcc gggagcggcg agccggcgag gggtgggtgc tcaagccggc
     4321 cctcggcagg gtgggggacg gcatcggcct ggcgggcgtg acgccggggg gtgcgctcac
     4381 gaagatccgc cgcgaagcct ggtggttccc ctcctggtgg gcggcgcagc ggcgcttcga
     4441 ggccgtgccg caccggttcg gagagcagga cgtctacccc tgcgtcgggg tctacaccgt
     4501 cgacggccgg gtggccgggg cctatggccg tgtcgcccgc ctccctctcg tcgatcatct
     4561 cgcccaggac gccgccgtgc tcgtcgaaga gtcccaggcc gaatcgccgc ggcagggcgc
     4621 cgcgaaggag atccatgaac gcgcagcagc tctatgacat ctgggccccg gcgggctccg
     4681 tgtggtccca atgggcgaag ccggccctgt tcgcgggctt cggctcgccg gctccgtccg
     4741 ctgcccccgt gcctcccgac ctgccgccgg cgggagcggg gagcggaggg gagagcccgg
     4801 tgctcgcggc caccaacgac cagcggacgg cgtacgtcgt cgacctcccc ggcccccggt
     4861 cggtggaggt tggggccgac ctcgcccgcg cgggctaccg gccggtgccg ctgttcaaca
     4921 ccgccttcca cccggcggcc gtggtctccg tcggcgcgat cctggagcgg ctcgcggcgc
     4981 gggcggggga gctcgaggcc cggggcctgc cccccgaggc gccgccggcc ttccttctgg
     5041 actcgcggcg gctgaacccg ggcctggtgc agactcccgg ccagttcgac aaccgctggg
     5101 ccacctttcc ccaggacttc ccctcggcga gcttcctcct gtcgcggggc atccagaggg
     5161 tggtcctggt ccacgacggc ggggagccgg acagcctcga cgacctcgcg catgtgctcc
     5221 tccgttggca gcgggccggg atcgagatcc tgcgccagga cacgtcggcc ggcgcgtccc
     5281 cgcagccgtt cgaggtccga aagccgctcg ccttccggag cctcctctac cgggctctca
     5341 cggcggccgg cctgcgccgc aacagcgccg gcggcttcgg cgcgatcatc ccgctcgcca
     5401 ccagcagcgg ctagcttccg agccgccccc cgcttcaccg gaccgtcctg actggaccct
     5461 gactggacgg ccatgggtca tcccggatat ttttcccgca accaggcgcc ggctcttgct
     5521 gctccggagg gacagagacc tccgaagcag cgccttcggg agccggcggg atacccagtt
     5581 gtccgggagg gaggagccga ccatggccag cctgtatcag cgcgtcgcgg cccacgccat
     5641 ccgtctccgt ctccgctcca ggggccgcaa ccgggcgcgc cggaccaacc tcctcatcca
     5701 ggaggaggtg ctcgcggcgg gcagctccat cgcgacaccg ctggagacga tccacatccc
     5761 gcgcgacacg gtgctcgtct tcgccgacga cgacccggag gccaactggg cccacccctg
     5821 ccgctatctt ctgtacgacg ccgcggacgg cgagctgtat cagcaggtgc cggcgagctt
     5881 ccccccctat ctccatcagc cggtgcccaa cctgcagctc ctgcgcagcg agttcccgcg
     5941 cggcgtccgc tggcgtccca gccgcctgcc ctggcagccg cccgtcctgc tggtgcccgg
     6001 caaccgctac gccatcctct tctgtggcgt ggccaactac cggcatgtca acgacctgga
     6061 attcctctac cggaccctgc ggcagaccta cggctatccc acgaagcaca tccgcgtcct
     6121 cgagggcgac ggcaccctgg ggtacacgcc cacccccggc ctcgacccgc cgccggggga
     6181 cccgtggccg ggggacgtct cggcctatca gatgaaggtg gacggaccgg ggacgcgggc
     6241 ggcgctggcg tcggccctga acgcccttcg gcggcggctg aaacccaacg actcgctgct
     6301 gctccatacc gtcggccacg gctacacctc gggcgacgag tcgttcctgg tgacccacgc
     6361 gggtccgccc tgcgcggccg gcgacttggc ggagcggctc gcgcagctcc cgccgttcgc
     6421 ctgtctgacg gtgatgatga gccagagcgg cgcggggggc ttctgcgctc cgctcctggc
     6481 cgccagcccg gcgcgccgga ccacgttcgc cgccgcctgc ggccccctgg gcagctccta
     6541 cggctaccgc gacttcggct acttcgcccg cgactggacc gccgccatgc gcggggcgga
     6601 cccgtacgga gcgcctctgc ccatcgacgc cgacaccaac ggcgacggcc gggtggtggc
     6661 gaaggaggcc ttcgtcttcg ccaaccgtgt gcgctacgag gaggacagcc cgcgctacga
     6721 ggagacgccc gggtcgcggg gcggttgcag cctgaagccg ggatggcgac ccgcctggtg
     6781 ggacgacctg tggaaggagc tgctcctgcc ctggtggaag cggctgcctc cggaggtctt
     6841 ctatgggcgg ctctatgacg agctgatccc ggccgccggc aacctccggg agatccgcga
     6901 cgccggcctc gagggctggg tcacggtcca cggccgccgg ctgcgccaga tcgtcaccga
     6961 ggtcatgtcg cggccggtcg agtaggggtt acagcaccgg cgacagccag cgggccatgc
     7021 ggtcgccgat ctggcgggtg aggggacgct ggcgccagtc ctcgagcagg atctcctcgg
     7081 actcctccat gtcccggcgg aaggcgtcct ccatcaccgc ggcggtcccg gggtccagga
     7141 tcaccaggtc gcactccgag ttgaagagga acgagcggaa atcgaggttg gtggacccca
     7201 ccaccgagac gtggccgtcc gccaccaggc tcttggcgtg gagcaccgcc gactggtatt
     7261 cgaagatgcg gacgccgcgc tccaggagat cggtgaagta gccgtgcccg gcgcggcgga
     7321 ccacctggac gtcggtcttg ccgggcagga ggaggcggac gtccaccccg cgctgaacgg
     7381 cgtcgccgag gaggcggacc gcccggtggc ccggagcgaa gtaggcgttg gtgatccaca
     7441 ggttgcggcg cgccgccccg gcgatggccg ccagggccga agccgactcc gcctggccgc
     7501 ggttgggacg ggagtcgagg accaggatcc tcgcccccgg cgcctcggcc tcctcggcgg
     7561 acagcggatc gatgtcgaac ggagcgccgt cggcctcgat ccacccttcc gagaagacca
     7621 ccgccatctc ccaggccgtg gggccggcca cccggacgtg ggtgtcccgc cacgtggcgg
     7681 ccggccggcg cgcgaaggag ccgtactcct cgccgatgtt catgccgccg gtgaaggcga
     7741 cctcgcggtc cacgaccagg atcttgcggt ggtcccggaa cggctggtac cagagtttct
     7801 tgaacagggg attgaagagg cgcacctcca tcccctggcg ctccatctcc tgccagaacg
     7861 cggtcttcgt ctcgaacgag ccgagggcgt cggcgagcac ccggacggtg acgccgcgct
     7921 tcacggccgc caccatccct tcgaggaacc ggcggccggt cgcgtcatcc ttgaagatgt
     7981 aggactcgag caggatctcc ctctgggcgg cgagccccgc ctcgcccatg gagcggaagg
     8041 cgtcggcgcc gcggaagaag acctcgacct ggttgccacc cagcaccggc gcgccgtcga
     8101 tgcgctcgag cagcagctcg aacccggggt cgagcaggcc ctgcggcagc ttggcggcga
     8161 ggcggccgac ccggcgcgcg cggagctcac gggggagccg ccgtccgacg tgggggcgca
     8221 ggatcggccg gaagtgccgg aagcgtcggc ggcggagtct catggacggg cggagtatgg
     8281 cagagccggg ctgggatagc atgcgggcag gaggatcccg tgagagccat cgccacccga
     8341 ccgtccctga tcctgctggt cctctttcta accgcccagg cgctgtctgc ccaggtggcc
     8401 caactctcgg acgccgacct caccacccgc gccgagcgct tcgtcgatct cctcgcccag
     8461 gggggcttcg aggaggcggc gaaggggttc gacaacacca tgaaggccgc ggcgccgccg
     8521 gcgaagctgg ccgagatctg gaaggcgctc caggcgcagc tcggtccctt ccagaagcgg
     8581 accgccacgc gcgtcgagcc actgggaggc taccggtccg ccttcgtcac cagccgcttc
     8641 gagaaggacg ccgtcaacct ccaggtggtc ttcgacggcg aggggcggat cgccggcttc
     8701 ttcgtcaagc cggcccccgc tgcggccggc gcggctccgg ctccgggaac cacgccggcg
     8761 cttcccgcct acatcaagaa ggactccttc caggagaagg aggtggaggt cggggcgggc
     8821 gagtgggcgg tgcccggaac gctttccata cccaccggaa aagggccgtt ccccgcggtc
     8881 gtgctggtcc acggctccgg gcccaacgac cgggacgaga cgctcggtcc caacaagccg
     8941 ttccgggacc tcgcctgggg gctggcctcc aaggggatcg ccgtcctgcg ctacgagaag
     9001 cgcacccgct tccatggcga caagctggcc tcgctgccca aacccacggt gaaggaggag
     9061 acggtggatg acgcggtggc cgccgcgcag ctcctccggc gtaccgaggg gatcgatccg
     9121 cggcgggtct tcgtcctggg gcacagcctg ggtggcatgc tgatcccgcg gatcggcgcc
     9181 cgggacgggg agatcgccgg cctcgtcatc ctggcgggtc tcacccggcc gatggaggag
     9241 accatcgtgc agcagctctc ttacatcgcc tctctcgacg gcacggtcac ggacgaggag
     9301 aagaagcggc tcgatgagtt ccaggaacag gctgccaagg tcaagggtct caccccggag
     9361 tctaccgaga gcctgatggg catccccgcc tcgtactggc tggacctgcg gggctacaac
     9421 cctcccgcgg ccgcggccag gctgcgccag cccctgctga tcctccaggg cgagcgcgac
     9481 taccaggtga ccatggacga cttccaggcc tggaagaagg ggctggcgga gaagaagaac
     9541 gtgacgttca agtcctatcc gaacctcaat cacctcttca tggagggaga gggcaaggcc
     9601 acgccttcgg agtacgaaag gccgggccac gtcgccgcgg tggtgatcga cgacatcgcg
     9661 gagtgggtcg ggaaagcggg caccaccgcg aagtaggcgc ggcgttggag agtgaggccg
     9721 tatactcgct tccatgagga gatcgggccg ccttctcgga tctctccttc tcaccgcgct
     9781 tgtcgcgtcg gtggtcgcgc cggtccctgt ccgcgccgcg gacctctcct tctcgcgcac
     9841 cgtcatcgct ccggacagcc cggagtgccc gtgcggcaag gcgctggggg atgtcaacgg
     9901 cgacggccgg ccggacgtga tcgtcgccgg caacgccggg ccgctggtct ggtacgccaa
     9961 tcccgattgg gccgggtcag tgatcgccgc cggcgggttc accgcccagg gcggcctcgc
    10021 ggccggcgac ctggaccggg atggcgacct cgacgtcacc gtgggcaccg tctggttcga
    10081 gaaccccggg gggaatccgg cggccgcgcc ttgggaggct caccgcatgg tccccggcgg
    10141 cggcgcgcgg gaggtcgtga tcggcgacct cgatcgcgac ggccggcccg aagtcgtcct
    10201 gcggggagag actgcttcgg tggtcacgct cctcaggcac aacgacccgt cgttctggac
    10261 ccggcgcgac ctcgccgccg gtgccggatc tcgcggcctc gcgctgtccg acatcaacaa
    10321 ggatggcttc cccgacctgg tcgccggcgg gcgctggctg aggaatccag gggggaacgt
    10381 gctctcccgg ccctggtccc gccgcaactt cgcgtcctgg agcccggagg cgtccgtcgc
    10441 cgtgggggat ctgaaccggg ataaccggcc ggacgccgtg atgacgttcg ccgggagggc
    10501 gggaaggatc tcctggttcg agagcccgcc caatccggcg aagccgcgct ggccggagcg
    10561 ggtcatcgac cgccgcgtcg acacggcgcc aggggtgagc ctcgtggaca tcgaccgcga
    10621 cggcgatccc gatgtggtga cgtcggagtc cagggggcag gggcgcctcc tgatctatcg
    10681 caacgaaggg cagggcgcgc gctggacgcg tcaggttgcc ggcacgcccg ccctgcacga
    10741 catccgtgcc gccgatgtcg acggggacgg agacgccgac atcctcggca ccatgccgtt
    10801 cgggagcggg ccggtggagc tgtgggaaaa cgagaccgag gtgcaacccg tggtgaaccg
    10861 gatcctcgtc ttctccaaga ccgccggctt ccgtcactcc agcatcgagc cggggatcgc
    10921 ggccatccgc agcctgggcg ccgcgaacgg cttcgaggtg gacgccaccg aggacgccgg
    10981 ccagttcacc ctcgcgaacc tgaaccgcta taaagccatt gtcttcctgt ccaccaccgg
    11041 cgacgtgctc aacgaggcgc agcagaacgc tttcatgagc tacatccgcg gcggcggcgg
    11101 ctacgtgggc gtccacgcgg cggccgacac cgagtacggc tggtcctggt acggagatct
    11161 gatgggggcc tacttcgcgc ggcatccggc gccggccccg gcccgcatcc gggtcgagca
    11221 ccggaaccac ccctcgacaa gcgcgttgcc ggacccctgg aaccgcaccg acgagtggta
    11281 cgacttccag aggaacccgc gcggccgggt caccgtgctg ctgaccctgg acgagtcgag
    11341 ctattccggc ggccagatgg ggaacgatca ccccatcgcc tggtatcacg aattcgacgg
    11401 cggccgcgcc tggtacaccg ggggcggcca caccgacgag tccttctccg agccgcagtt
    11461 cctgcagcac ctgctgggcg ggatccgcta cgcggcgggg ctgtgagatc tcgttcaggg
    11521 agctcccctg gccctcctgc ccagccgctg tcggccccct actccggcga ggccgctccc
    11581 tgcggttccg gcaggtagag gccggcgccg ggacccatcg gcaggtcgaa cagcatccag
    11641 acggccagga ggagcgccca ggccagcagg aagaccacgg tgtaggggag catcgtcgag
    11701 atgaccgtac cgatgcccgc cttgggatcg taccgtgcca cgaaagcgac gatcagggcg
    11761 aagtaggtca tcatcggcga gacgaagttg gtggtgctgt caccgacgcg gtaggccgcc
    11821 tgggtgagct ccggcgtgaa gccgagcagc atgaacatgg ggacgaagac gggcgccatg
    11881 atggcccact tggcggaggc gctccccatg aacaggttga tgaacgcggt gaacagaatg
    11941 aacaccagca tcagcgggat cgggccgagc cccgacgcct tcaacagcgc ggcgccctcc
    12001 acggccagga tcagcccgag gttggtccag ttgaagaagg ccacgaactg cgcggcaaag
    12061 aagaccagga cgagatagag ccccagcgtc tccatcgcct tgcccatgcc cttcatcacg
    12121 tccgcgtcgc tcttgaacgt ccgggcgccg aaaccatagg ccaggcccgc gatggcgaag
    12181 atgaaaaaga tcaggcttac gatgccggtc atgaacggcg accgcaacag gctcccggtc
    12241 tccgggtcgc gcaggaagcc cccggccggt atggtgccca gcaggaggat gacggccacc
    12301 gccgcgagcg cggccaaggt gaaccagagc ccgcgcttct cctcgggtgt caggcggttc
    12361 agcgcttccg gcttctcgcc gccccggtac tcgcccaggc gcggcgccac gatcttctcc
    12421 gtcacccagg tgccggccgc ggtgactacg aacgtcgaga cgaacatgaa gtagaagttg
    12481 gccgtggcgt tcacctcata ggcggggtcc acgatatgcg ccgcttcctc ggagaggccg
    12541 gacaggagcg gatcgatggt gcccagcagc aggttggcgc tgtagccgcc ggagacgccg
    12601 gcgaaggcgg cggcgagccc cgccagggga tgccggcccg cggcgaggaa gatgatggcc
    12661 gacagcggca ccagcagcac gtagccgacc tcgctggcgg tgttcgacag cacgccggca
    12721 aacacgatca ccacggtcag caggcgccgg ggcgccgaca tcaccagcag gcggagcgcc
    12781 gtgccgatga cgccgctggc ctccgcgatg ccgattccca gcatggccac cagcactgtg
    12841 cccagcggcg cgaagccggt gaagttgctc accatggagg tgaggatccg gtggagcccg
    12901 gcgatgctca acaagttgac cggccggatc tcctcccccg tgccagggtg gatgaccatc
    12961 aggtccagac gggagaacag gcctgacagg aggacaactg ccagggccat cagagcgaag
    13021 agagtgccgg gatgcggaag cgcattgccc acccgctcga tcagggccag actgcgttgc
    13081 agcaacggct gccggggtgc cgcttgactc ggcatgtgat ctttctccct tcccgcccgg
    13141 gggcgattga ggcggcgtag tctaccaaag ccggccgccg gcaagggtca cccgccgcag
    13201 gcgcgggcga gcagcatcac gatcaccacc agcccgagca gcgtgccgcc gagcagccag
    13261 gggcgccgcg ccggcggcac cagatcgcgc gcgatgtcgc cggcgacgcg ggcggcgaat
    13321 tcggcctggg tggggacagg cgccggtggg ggcgccgccg ccgccggagc ccccacgtcc
    13381 gccggcacgg cggcggcctt caaggcctgg agctcgcggg cgagaccctc cagcccctgt
    13441 ttcgtgaccg agcgtgccga gctgtcgagc acgcgctggc cgacgcccgc gacccggccg
    13501 cccacctgag tatcgaggtc gtagcgcagc accgtgccgc cgctcccatc cgcggcatcg
    13561 gcgagacgca gatccccctc accgttcatg aagccggcgg ggccgctccc ttcgagccgc
    13621 atgtgatagc tctcgagcgg caccaggtcc gacagccgca gcgtcccctg gaactggccg
    13681 cggaccgggc cgacctggat gttcagcacg ccccgcagct cgtcttcatt caccttttcc
    13741 aaccgctcgc agcctggaag ggtccgcgcc agcacggcgg ggtccagcag cgcctgccag
    13801 accagctcgc gcggcgcgtc gaaggtgtgc tccccctgga tcttcatgat gccctcctga
    13861 tctgcgcgtg ccgcgcctcc tcgctcagct cccacagccg gttcggcgag agcgggatct
    13921 ccaggatctc gaagccgggg atggccagcg cgtcctcgac cgcctgggcg aagagcgcgc
    13981 ccaccgggat ggctccggct tcgccggccc ccttggtgcc cagctcgttg agcggcgaga
    14041 cggtctcctc gtggccgacc tccacccgcg gcacgtcgag cgcggtggga agaaggaaat
    14101 ccatgaacga agcgttcagg agctggccgt tgtcgtcgta gtggagctgc tcgaagtacg
    14161 cgttgccgat cccctgcgcc acgccgccgt ggacctggcc gtccacgatc agcgggttga
    14221 gcacccggcc gcagtcgtgg acgacgacat acttcttgat ctcgacctgc atcgtctccg
    14281 gatcgacctc caggatgcag gcatggcagc cgctggccgt ggcgccgcgg aaggggccga
    14341 agtagcgcgt cgcctcgaga cccggctcgg tgcccggctt gaccgcgccg cgcatcgggt
    14401 tcgccagctt cgcaagctcg ccgagcgaca catgtcggcc gtgatcgtcc ttcacacgcg
    14461 ccttgccttc cgccagctcg atccggtcct cggcgacgcc gagatgctgg gccgcgagac
    14521 cgatcgtttt ttcgcgcacg gccttcgccg cctcgttggt ggcgttgccg gcgaccactg
    14581 cgccgcggct ggcgaaggtg ccggtccccc aatgaaattg atcactgtcg ccggtgacca
    14641 cgtccacctg atccgtgtcc accccgagct gctgcgccgc gatctgcgcg aggaccgtga
    14701 agtgcccctg gccctgcgtg ccgacgccgg tgacgacggt gaccttgccg ctcgtctgca
    14761 cctgcacccg cgccccttcg aacggcccga tgcctgtccc ctcgacgtag gagacgacgc
    14821 cgatccccac cgccttgccc tcggcccgca ggcgcggctg ctcctcggcc aggaaccgct
    14881 catagccgat ccgctcgagc gtctgggcga gcaccggcgc gtagttgccg ctgtcatagt
    14941 agagcggcgc gaaatcctga tagatgatca cgtggtcgtg cgggaactcg tcggggcgca
    15001 ggaagttgcg gcgccggagc tccatcccgt cgatgccgag ctcgcgggcg gcgatgtcca
    15061 tcaggcgctc gatgacgaag actccgtgct ggcggccggc gccgcggtac ggggtgacga
    15121 tcgtcttgct ggtgaacacc gcccggaact cgctggcata ggccggcacc cggtagggcc
    15181 ccatgagcgt gcactggctg ttcaacggca cggtaaggcc gtagggatcg taggcaccgg
    15241 tgtcgtgcag gaacgagtcc ttgaccccga ggatgcggcc gtcgcgagtg agggcgatct
    15301 cggcgtcgtg gatctggccc cgctcctggg tggtccccac gaagttctcg gcccggtcct
    15361 cgatccattt caccggccgt tcgagccgca gggcgagcca ggggatcagc acctcttcgg
    15421 gatagaacat catgatcttg ggaccgaagc cgccgccgat gaacggcgcc accagccgca
    15481 cttgatgctc ggacaggccg agcatcgccg cgaggccgtt gcgcaccggg atcggcgcct
    15541 gcgtggtgtc ccagatgctc aggcgctggg cccgcgcgtc ccactgcgcc acgacgccgc
    15601 ggttctcgat cgccgccgac gcgccgcggt cgtaggagaa gcgccggcgg accaccaggt
    15661 cggcctgcgc gcgcgccgct tcgtagtccc ccttcttctg gactacgtgc gcggcgacgt
    15721 tcgatcccac gtcctcgtgc agcagcgccg cgttggggga cagcgccttc tcgagatcga
    15781 cgaccgccgg cagcggctcg tagtcgacaa agatccgctc gacggcgtcc tcggcgatat
    15841 agcggctctc ggccaccacc aggacgacgg gctcgccgag gtggcggacc ttgtccttcg
    15901 ccagggggac ctgcgtccgc tggtgaaaaa ccatcccttc aacgggcggc ggcggcacga
    15961 ggaggggccc cggctgccag tagtcgccga ggtccgccgc cgtgtagacc gcgaccaccc
    16021 cctccatctc gcgcgccgcc gagacgtcga tgccacggat gcgcgcgtgg gcgtgcgggc
    16081 tgcgcaggaa cgccacatgc gccatgccgg ggaggttcaa gtcatcgacg aacagcgccc
    16141 ggcccgtgag caaccgcggg tcctcgttgc gcttgaggcg ctgaccgatc acttttcccc
    16201 cgccttgtcc tcgacgaacg ccacgatgcg cgagaacgcc gcctcgcccc ccttgaagcg
    16261 gaacgggaag cagccgatcc agaccttctg gtcgagcacc tcgtcgatct ggccgccgat
    16321 gttctcggcg tggaagacac cgtgcgggaa gag
//
LOCUS       DASPJI010000096         3940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_807204, whole genome shotgun sequence.
ACCESSION   DASPJI010000096 DASPJI010000000
VERSION     DASPJI010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807204"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..498)
                     /locus_tag="VEL74_02565"
     CDS             complement(244..498)
                     /locus_tag="VEL74_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61439.1"
                     /translation="MMQAFRTKAVLFSLACLLTATALFLPHSSEASVRQCLRHLEYCT
                     YSTGGFCRDDCVRGHVCRLDVSGEVVSCTVTTVDCCAGGA"
     gene            complement(572..931)
                     /locus_tag="VEL74_02570"
     CDS             complement(572..931)
                     /locus_tag="VEL74_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61440.1"
                     /translation="MEDRRWSRFVAAVVCILALIALAPQVARAEEGGAPVVATAVMEA
                     ATVEQPEPVKPADLSWLGEATAKVEPPAGALGGALFLGEVPTHGCPFSCGSTNCYSHC
                     ALQGYTGRCINGCCNCF"
     gene            complement(1079..2260)
                     /locus_tag="VEL74_02575"
     CDS             complement(1079..2260)
                     /locus_tag="VEL74_02575"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HYG61441.1"
                     /translation="MLGALANLLVLGLVGLSGAAMAGLVARAVAGSRERQKEWHEVVT
                     RCGLVPMKTDRWTTLLSLQAQGGPVEILAHEEAGGIQLEATVKGAPPDLADVVIRRET
                     LKLPGTREVEIGHDVFDSAFLVQGTPRYLAALLDARTRRMLLEAGAETALSLSGGRLR
                     AQVKWDQLARVLPLVVDLGRRLAEITDVPRSLEENVRRDPHPGVRLHSLLLLLQDYPG
                     YPSTEEALRIAREDDSAQVRLEAARELRDVATLAGMMARDSFELAVAAARALGETRNP
                     AAEPPLLEALARDNPALQAAAAAALGRAGSAGAVPPLLDLAESPSSPRELRRAARQAV
                     AEIQSRLAGATPGQLSLAATDAGQLSLAPAQAGQLSIAPHAVGELTVPPPPGVRTRSG
                     G"
     gene            2404..2733
                     /locus_tag="VEL74_02580"
     CDS             2404..2733
                     /locus_tag="VEL74_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HYG61442.1"
                     /translation="MGKPNDLVQGTLDLLILKTISLEPMHGWGIAKRIQQISQDVLRV
                     QQGSLYPALHRLEQQGWIDSQWGEAENGRKTKVYSLTRAGREQLEKELANWDRLSNAI
                     HLVTRMA"
     gene            2743..>3940
                     /locus_tag="VEL74_02585"
     CDS             2743..>3940
                     /locus_tag="VEL74_02585"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG61443.1"
                     /translation="MRSTDKMQLRLRSLFLRPRVEAELDAELAFHIDQLTAEHVAAGM
                     SPREARHAAMREFGGVTQIQERVRDTWGIRWLEQLLQDLGFALRTFRRSPTLTLVALL
                     SLALGIGANTAIFSMLNAFLLRPLPVHDPAGLVAVGDPTRVHDLSEGTVRTDLFSYPL
                     YRELRERNRVFTGMFASGDAGRLTVGDASAAGAETVRGRLVSGNFFAVLAVDALLGRT
                     FTEADDRAPGAAPYVVLSHRYWERRFAADPRAVGQTLTLNGHPFTIVGVTPPEFFGDV
                     VGGSNDLWIPLSMQPQVHPGRMYLDRWDIGWLLLMGRLQPGASIERARAEMNTLVPRV
                     LAGLSAAEVPAELIPSPDQVRIDVTPGSGGFSRARREMTQPLLMILAIVFLVLVVACA
                     NIANLLL"
BASE COUNT          590 a         1425 c         1357 g          568 t
ORIGIN      
        1 gtgggggagg aaaagggccg tggcggtcag caggcaggcc agcgagaaca gaacggcctt
       61 ggtccggaac gcttgcatca tggcaacctc cttggcccag tgctgggccg tttactgaaa
      121 aggtgcctgg gagcgccggg ccgcgccggt ggcatatagg ctgccggccc gcgctctgag
      181 atccgcgtta gctggtaccc aaggatcgcc gccgtgtgaa gaaagatcct cctgtccccc
      241 ggcctaggcc ccccccgcgc agcagtccac ggtagtgacg gtgcagctaa caacctcacc
      301 gctgacgtct agccggcaga cgtgacctct cacacagtcg tcccggcaga acccgccggt
      361 cgagtaggtg cagtactcca aatgtctcaa gcactggcgg acggaggctt cggaggagtg
      421 ggggaggaaa agggccgtgg cggtcagcag gcaggccagc gagaacagaa cggccttggt
      481 ccggaacgct tgcatcatgg caacctcctg gcccagatgt gggcctttat cgaggggagg
      541 cccgtccggg cctcccctcg tgggtgtgtt tctagaagca gttacagcag ccgttgatgc
      601 agcgcccggt gtaaccctga agggcacagt gggagtagca gttggtgctg ccgcaggaga
      661 aggggcagcc atgcgtgggg acctcaccca ggaacagcgc gccgccaagg gctccggccg
      721 gcggctccac cttcgcggtg gcctcaccca gccaggacag atcggccggc ttcacgggtt
      781 cgggctgctc caccgtggcg gcctccatca ctgctgttgc caccaccggg gcgcccccct
      841 cctcggcccg cgccacctgc ggcgccaggg cgatcagggc caggatgcat acgacggctg
      901 cgacgaaacg gctccagcga cgatcttcca cacacacctc ctcgaaagcc cgaagttggg
      961 ctgtccctag agaaaggtgc atgagtgggc cggggccgcg ccgcggctcc cgggctcgag
     1021 ctgctccagt cggccctggg cgatctcacc cgcaaccctg ccggcgagcc gctgagcatc
     1081 aaccgccgct tcgggtccgg acgcccggcg gcggggggac cgtcagctcc ccgaccgcgt
     1141 gcggagcgat agagagctgc ccggcctggg ccggcgccag ggagagctgg ccggcgtcgg
     1201 tggcggccag cgacagttgg ccgggtgtgg ccccggcgag ccgggactgg atctcggcga
     1261 cggcctggcg cgccgcccga cgcagctcgc gcggggagga ggggctctcg gccagatcga
     1321 ggagaggcgg cacggcgccc gccgagccgg cgcggcccag cgccgccgcc gcggccgcct
     1381 ggagcgccgg gttgtcgcgc gccagcgcct ccaggagcgg cggctcggcg gcgggattgc
     1441 gcgtctcccc cagggcgcgg gccgcggcga cggccagctc gaagctgtcg cgcgccatca
     1501 tcccggccag ggttgcgacg tcgcgcagct cccgggccgc ttcgagacgg acctgcgcgc
     1561 tgtcgtcctc acgggcgatg cgcagcgcct cctccgtact cggatagccg gggtagtcct
     1621 gcagcaggag gagcaggctg tgaagccgca ccccggggtg ggggtcccgg cggacgttct
     1681 cttccaagct ccgcggcacg tccgtgatct cggccagccg gcggccgagg tcgacgacga
     1741 gggggaggac acgggcgagc tgatcccact tgacctgggc acggaggcgc ccgccggaga
     1801 gggacagcgc ggtctcggcg ccggcctcca gcagcatccg gcgcgtccgc gcgtcgagca
     1861 gcgcggccag gtaccgcggc gtcccctgga cgagaaaggc ggagtcgaag acgtcgtggc
     1921 cgatctcgac ctcccgggtc cccggcagct tgagcgtctc gcggcggatc accacgtccg
     1981 cgaggtccgg cggcgctccc ttcaccgtcg cctcgagctg gatccccccc gcctcctcgt
     2041 gcgccaggat ctccaccggc cccccctggg cctggaggct caggagcgtc gtccaccggt
     2101 cggtcttcat cggcacgagg ccgcaacgcg tgaccacctc gtgccactcc ttctggcgtt
     2161 cgcggctgcc cgccaccgcc cgggcgacga gcccggccat ggcggccccc gagagcccca
     2221 cgagccccag cacgagcaag ttggcgagcg cacccagcat tccgtacctc ccggaacccc
     2281 gtcctatgac ctcggacctg cctccattat agggagacgc ggggagccgc gaagaacccg
     2341 ccggcagata ccttgacagt ctacaattct taggagtatt gtcgaccgtc gaggtatgaa
     2401 cctatgggaa aaccgaacga tctcgtccaa ggaaccctgg atctcctgat cctgaaaacg
     2461 atctctctcg aaccgatgca cggctggggc atcgccaagc gcatccagca gatctcccag
     2521 gacgtcctgc gcgtgcagca gggctccctc tacccggcgc tgcaccgtct ggagcagcag
     2581 ggatggatcg actcgcagtg gggggaggcc gaaaacggcc gcaagaccaa ggtctactcg
     2641 ctcacgcggg ccggccggga gcagttggag aaggagctcg ccaactggga ccggctgtcc
     2701 aatgccattc acctcgtgac gcggatggct tgaggaccgg cgatgcgctc gacagacaag
     2761 atgcagctgc gcctgaggtc cctcttcctg cgcccccggg tggaggccga gctcgacgcc
     2821 gagctcgcct tccacatcga ccagttgacc gccgagcacg tcgccgccgg catgagcccg
     2881 cgggaggccc gccatgccgc gatgcgcgag ttcggcggcg tcacccagat ccaggagcgt
     2941 gtccgcgata cctgggggat ccggtggctc gagcagctcc tccaggacct gggcttcgcc
     3001 ctgcggactt tccgccggag ccccaccctg acgctggtgg cgctgctctc cctggcgctg
     3061 ggcatcggcg ccaacaccgc catcttcagt atgctcaacg ccttcctcct ccgcccgctg
     3121 ccggtccacg atccggccgg cctggtggcg gtgggcgacc ccacgcgggt ccacgacctg
     3181 tcggagggga cggtccgcac cgacctcttc tcctatcctc tctatagaga gctccgcgag
     3241 cggaaccggg tgttcaccgg catgttcgcc tcgggcgacg cgggccggct gaccgtcggc
     3301 gacgcctcgg cggccggggc ggagaccgtg cgcggccggc tggtcagcgg caacttcttc
     3361 gcggtgctgg cggtggacgc gctgctcggc cgcaccttca cggaggcgga cgaccgggcc
     3421 cccggcgccg cgccctacgt cgtcctcagc caccgctact gggagcgccg cttcgccgcg
     3481 gacccgcggg cggtcggcca gacgctcacc ctcaacgggc acccgttcac catcgtcggc
     3541 gtcaccccgc cggagttctt cggcgacgtg gtcggcggct ccaacgacct ctggatcccc
     3601 ctgtcgatgc agccccaggt gcaccccggc cggatgtacc tcgaccgctg ggacatcggc
     3661 tggctgctgc tgatggggcg tctccagccg ggcgcgtcga tcgagcgcgc gcgggccgag
     3721 atgaacaccc tggtgccgcg cgtcctcgcc gggctctccg cggccgaggt ccccgccgag
     3781 cttatcccct cccccgacca ggtccgcatc gacgtcaccc cggggagcgg cggcttctcg
     3841 cgcgcccggc gggagatgac gcagcccctg ctcatgatcc tggcgatcgt cttcctggtg
     3901 ctggtggtgg cctgcgccaa catcgccaac ctgctgctcg
//
LOCUS       DASPJI010000097         4805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_5344, whole genome shotgun sequence.
ACCESSION   DASPJI010000097 DASPJI010000000
VERSION     DASPJI010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5344"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VEL74_02590"
     CDS             <1..713
                     /locus_tag="VEL74_02590"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HYG61444.1"
                     /translation="GVWIGRESAARNAEKLAANAAAPAAQEPQEGRSVQEYEFFEQPQ
                     AEGQAPQEEPTAPAPAEGQDTTLLEDMGGEDEDFPAMEDEQEAAPAAVPSPSPTPVQR
                     PTAEERRLARQRERQAQQAAQQPAPARPEDTVRPRPLPEATPASPASRAAAPAAASTG
                     GGRVVIQVFSSPERDQAERIQKRLTSGGQKAFLSPVDVGGRTMYRVRIGPFANREAAQ
                     QVAEQVRKGYKLDTWVTE"
     gene            751..2277
                     /gene="lnt"
                     /locus_tag="VEL74_02595"
     CDS             751..2277
                     /gene="lnt"
                     /locus_tag="VEL74_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HYG61445.1"
                     /translation="MRRFLLWALVAALGGWMWALCFEREPLTVAPWLALAPLFLLLRS
                     PWPGRLAFLHGLVAWMVSLSWISPTLQDFGNIAPPLSVVLTGLLSAYLAAFHTAFAWL
                     GAPLWRRGGALRFVALPALWVALEWLRTHLFGGFPWNLAAYAWVDVPGALPLSAWVGA
                     YGISFLVLLASAGVAACVEAWSPREEGRRRRSWQPLAACLGIPLLLLPLAGRWSVRQT
                     DNDFREIVGLGGPIRLLQPNIPNLVSLDPVKVFENYGKVISHSVAACQPGSLVIWPES
                     AAWPFQYGRDPHFEQDLRMLVERGCTVLFNSSHPAGNSFYNSAFLLSPGDAPVARYDK
                     RHLVPFGEFIPFKGLFSWMDKLARNAGDFLAAEKMTLLPWNGERLGMAICYEVVYPGE
                     VADLTKAGATILVTITNDAWYGDTAAPWQHFRAARFRAAENRRPLLRAAITGVSALVA
                     SDGSVRASIDVFKEGVIRGWVVGRTGLTPYARATWAVPVACTAVALFGLYFGRRRRTS
                     "
     gene            2274..3393
                     /gene="prfB"
                     /locus_tag="VEL74_02600"
                     /pseudo
     CDS             2274..3393
                     /gene="prfB"
                     /locus_tag="VEL74_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_152411680.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            3442..4533
                     /locus_tag="VEL74_02605"
     CDS             3442..4533
                     /locus_tag="VEL74_02605"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HYG61446.1"
                     /translation="MHSREEGRARELVLRYGWNAMAYQILNPGLRYWFSADGEAVIAY
                     VVANGYRVVAGAPVCPPELLADAATAFGEEAHGVGQRVCYFGAQDRLIDLLAERGPAS
                     KVLLGAQPFWDPRHWPAVVAGKASLRAQLARARNKGVTVRFWPTDGTADHPALRRCLE
                     EWLQTRGLPPMHFLVEWDILPHLLDRRLFVAERDRQPIGFLIASPVPLRHGWLIEQII
                     RGRSAPNGTTELLVDAAVRALAAEGAEYVTLGLSPLSPVLDQHPPPPRSLRLLLGWVR
                     LHGRRFYNFEGLDRFKRKFQPDGWEPIYAVTDQRRITARTLYAIAGAFGGTSPILFIG
                     RASLRALWQEARWAAGKARRRLAPSSTRP"
BASE COUNT          699 a         1766 c         1636 g          704 t
ORIGIN      
        1 cgggggtctg gatcggccgt gagagcgccg cccgcaacgc cgagaagctg gcggccaacg
       61 ccgccgcgcc ggcggcgcag gagccgcagg aggggcgctc cgtccaggag tacgagttct
      121 tcgagcagcc ccaggccgag ggccaggcgc cgcaggagga gcccacggcg ccggcgccgg
      181 ccgaggggca ggacaccacc ctgctcgagg acatgggcgg cgaggacgaa gacttcccgg
      241 ccatggaaga cgagcaggag gccgcgcccg cggcggtgcc gtcgccgtcc ccgacgccgg
      301 tgcagcggcc gactgccgag gagcgccgcc tggcccggca gcgggagcgg caggcgcagc
      361 aagccgccca gcagcctgcc ccggcccggc ccgaggacac cgtccgcccg cgcccgctgc
      421 ccgaggccac gccagcgtcg cctgcatcgc gggctgccgc gccggccgcc gcgtctacgg
      481 ggggcggccg ggtggtcatc caggtcttct cctcgcccga gcgggaccag gccgagcgca
      541 tccagaagcg tctgacgagc ggcggccaga aggcgttcct ctctcccgtc gacgtgggcg
      601 gccgcaccat gtaccgggtg cgcatcggcc cgttcgccaa ccgggaagcg gcccagcagg
      661 tcgccgagca ggtccgcaag ggttacaagc tcgacacctg ggtcacggag tgagcggccc
      721 cctcccgggg ccgcctccca gctcctcctt atgagaaggt tccttctctg ggcgctcgtc
      781 gccgcgctcg gcggctggat gtgggcgctg tgtttcgagc gcgagccgct gacggtggcg
      841 ccctggctgg cgctggcgcc gctgttcctg ctactgcgct ccccctggcc ggggcgcctc
      901 gccttcctcc acggcctcgt cgcctggatg gtgtcgctgt cctggatctc gccgacgctg
      961 caggacttcg gcaacatcgc gccgccgctc tcggtggtgc tgaccggcct cctcagcgcc
     1021 tatctcgccg ccttccacac cgccttcgcc tggctcggcg ccccgctgtg gagacgcggc
     1081 ggcgctctgc gtttcgtggc gttgcccgct ctgtgggtgg cgctggagtg gctacggacc
     1141 catctcttcg gcggcttccc ctggaacctc gccgcctatg cctgggtcga cgtgccggga
     1201 gcgctgccgc tctccgcctg ggtcggcgcc tacggcatct cgttcctggt gctcctcgcc
     1261 agcgccgggg tggcggcctg cgtcgaggcc tggagcccgc gggaggaggg gaggcgccgc
     1321 cggagctggc aaccgctcgc cgcctgcctg gggatcccgc tgctgctcct gccgctcgcc
     1381 ggccgctgga gcgtgcgcca gaccgacaac gatttccgcg agatcgtcgg gctgggcggg
     1441 ccgatccgcc tcctccagcc gaacatcccc aacctggtga gccttgatcc ggtcaaggtg
     1501 ttcgagaact acggcaaggt gatctcgcat tcggtggccg cgtgccagcc gggatcgctg
     1561 gtgatctggc cggagagcgc ggcctggccg ttccagtacg gacgcgaccc gcacttcgag
     1621 caggacctgc ggatgctggt ggagcggggc tgcacggtgc tcttcaactc cagccacccg
     1681 gcgggcaact ccttctacaa ctcggcgttc ctgctctcgc cgggggacgc gccggtggcg
     1741 cgctacgaca agcggcacct ggtgccgttc ggcgagttca tccccttcaa gggcctcttc
     1801 tcctggatgg acaagctggc gcgcaacgcc ggcgacttcc tggcggcgga gaagatgacg
     1861 ctcctcccct ggaacggcga gcggctgggc atggcgatct gctacgaggt cgtctatccg
     1921 ggcgaggtgg cggacctgac gaaggccggc gccacgatcc tggtgaccat caccaacgac
     1981 gcctggtacg gcgacaccgc ggccccctgg cagcacttcc gggcggcccg gttccgcgcc
     2041 gccgagaacc gccggccgct gctgcgcgcc gcgatcaccg gcgtctcggc cctggtcgcc
     2101 tcggacggct cggtccgcgc ctcgatcgac gtcttcaagg agggggtcat ccgcggctgg
     2161 gtcgtcggcc gcaccggcct caccccctac gcccgcgcca cctgggccgt gcccgtggcg
     2221 tgcacggcgg tcgccctctt tgggttatat ttcggccgaa ggagaagaac ctcatgatcg
     2281 cttccgatac gcagcagcgc attcagaccc tcgccgagca gctctccaac ctccgggggt
     2341 atctttgagg ggacgggcct cgaaaaggac ctgtccgaca tcgaccggca gatgcaggag
     2401 ccgggcttct gggacaaccc gacggccaac gcctccctcc tccagcagcg ccgcgccctc
     2461 gagcgccgcc tcgagaccct gaaccgcctc cgcgccgacg ccgacgagct cgccacctgg
     2521 aaggagctcc tggccgaagg cgaggcggat gaggacttca gccgcttcat cgagcgcctg
     2581 gaagccgacg tcgagaagct ggagctggag ctcaagctct ccggcccgga cgacgacaag
     2641 aacgccatcg tcaacatcca ccccggcgcc ggcggcaccg agtcccagga ctgggccgaa
     2701 atgctcctgc gcatgtacct gcgctgggcc gaggaacacg acttcgacgt cgagctcatg
     2761 gagcgccagg acggggaaga ggccggcatc aagagcgcca ccttcgcggt gcgcggcgcc
     2821 aacgcctacg gctatctcaa gagcgaggcc ggcgtccacc gcctggtgcg gatcagccca
     2881 tacgacttcc aggcgcgccg ccacacctcc ttcgcctcgg tggacgtcta ccccgaggtc
     2941 gacgacgagg tggtcatcga gatcgccgac aaggatctgc ggatcgacac ctaccgctcc
     3001 tcgggcgccg gcggccagca cgtcaacaag accgactcgg cggtgcggat cacccacctg
     3061 cccaccaaca tcgtggtcgc ctgccagaac gagcgcagcc agatcaagaa ccgggcgatg
     3121 gcgatgaagg tcctgcgctc acggctctac gacctggaga tgcagaagcg tcgggccgag
     3181 caggacgcgc gcgaggggct gaagatggac aactcctggg gcagccagat ccggagctac
     3241 gtcctccacc cctatcgcat ggtcaaggac caccgcaccg gcgccgaggt cggcgactcc
     3301 tcgcgcgtcc tcgacggcgg catcgacccg ttcatcgagg ccttcctcaa gggccagatg
     3361 gcgagcggca ccgacaaccc ggacgcgttc tagtccctca ccctgtcccc ctctcccgcc
     3421 tcccgggaga gggggagacc gatgcatagc cgcgaagaag gccgggcccg ggagctggtg
     3481 ctgcgctatg gctggaacgc catggcctac cagatcctca accccgggct ccgctactgg
     3541 ttctccgcgg acggcgaagc ggtgatcgcc tacgtggtgg ccaacgggta ccgggtggtg
     3601 gccggcgcgc cggtctgtcc tcccgagctc ctggccgacg ccgcgacggc gttcggcgag
     3661 gaggcacacg gggtggggca gagggtctgc tatttcggtg cccaggaccg gctcatcgac
     3721 ctcctggccg agcgcggccc ggcttccaag gtgctcctgg gagcgcagcc gttctgggat
     3781 ccacggcact ggcccgccgt cgtcgccggg aaggcctcgc tccgggccca actggccagg
     3841 gcgcggaaca agggggttac cgtccgcttc tggcctacgg acggaacggc cgatcatccc
     3901 gccctccgcc gctgcctgga agaatggttg cagacgcggg gcctccctcc catgcacttc
     3961 ctggtggagt gggacatcct gccgcacctc ctcgaccgcc gcctgttcgt ggcggagcgt
     4021 gaccggcagc ccatcgggtt cctgatcgcc tcgccggtgc cgctgcggca cggctggctc
     4081 atcgagcaga tcatccgcgg ccgctcggcg cccaacggca ccacggagct gctggtggac
     4141 gcggccgtcc gcgcgctcgc cgccgagggg gcggagtacg tgaccctcgg cctgtccccc
     4201 ctctcgccgg tgctggacca gcatccgccg ccgccccgga gcctccgcct gctgctcggc
     4261 tgggtccgcc tccacggccg gcgcttctac aacttcgagg ggctggaccg cttcaaacgc
     4321 aagttccaac ccgacggctg ggagccgatc tacgccgtca ccgaccagcg gcggatcacg
     4381 gcgcggaccc tgtacgccat cgccggcgcc ttcggcggca cctcgcccat cctcttcata
     4441 gggcgagcct cgctccgggc gctctggcaa gaggcgcgct gggccgccgg gaaggcccgc
     4501 cggcggctgg cgccgtcctc cacgagaccg tgaaacagtc cacgggcgcg gcgccgcgcc
     4561 tcccatccgg cggccgcgcc cgggaaccgg gtccatcgat tgtcaaagaa cgtccgtaac
     4621 cactctgccc tgctgttcaa ccaacgatcc catgagatca cgtgagaccg ggtaggtcaa
     4681 attcggtctt gtgtccggac cgattcctgg atagagtgac ccggagggcg ggcgatggac
     4741 cgatgcccat ctgcacgtgc tctttgaaaa ctgaatcgga cgggggcgcc gccgtgtcca
     4801 aggcc
//
LOCUS       DASPJI010000098         3034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_750014, whole genome shotgun sequence.
ACCESSION   DASPJI010000098 DASPJI010000000
VERSION     DASPJI010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750014"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="VEL74_02610"
     CDS             <1..1132
                     /locus_tag="VEL74_02610"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HYG61447.1"
                     /translation="IGGYGPHILGHRHPAIVAAIQEALGRGTAFGAPTLAEVELAEIL
                     TEALPALEMVRLVNSGTEATMSALRLARAATGRNRFIKFQGCYHGHGDSFLVAAGSGA
                     ATIGVPSSPGVPATVTADTLLAPYNDLEAVEALFQSGDAGDVAAVFVEPVAGNMGLVP
                     PAPGFLAGLRELCDRHGALLVFDEVMTGFRVAWGGAQNIFDIRPDLTTLGKVIGGGLP
                     LAAYGGRRDLMRRIAPAGPVYQAGTLSGNPLAVAAGRAALSILGQDVDCPYEQLEATG
                     ARLQAGLQEAAARHGIPCRVERQGSMIGLFFTDSPVRRLEDVDASDRERFKRVFHRLL
                     ADGVHLPPSPYEALFLSTAHGDAEIDLTLEAFDRALSQEAAR"
     gene            1143..2285
                     /gene="thiO"
                     /locus_tag="VEL74_02615"
     CDS             1143..2285
                     /gene="thiO"
                     /locus_tag="VEL74_02615"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HYG61448.1"
                     /translation="MSRPDVLVLGGGIIGLACARVLVREGLSVEVVERLSAGAEASTA
                     AAGMLAPLSETPVPGPFLDACRASRDLWPSWVAGLQEESGLAIDYDTGGAFHLALGEE
                     EETGLDRLALGAAEIGEPAVEVEPAVLAHRIPGVSPEVRRALHLLGEHRVDNIQACAA
                     LAVACRRLGVILSYGVHIEEVRRQGDGVRVMGHGYTRDAGCLVLAAGAWSGGVGGRGG
                     LPPLPVRPVRGQMMLLAGIDWPWKGTVRGTPFYAVRRGATGLLVGATVEEAGFSAHTT
                     PAGIESLLAFVRRYLPGLAGARLETLWAGLRPGTPDGLPILGPLPEWPVVVATGHYRN
                     GILLAPWTAEQVALLISAGAIGSAGASNEDDPFSPSRFLAEATVRR"
     gene            2348..2602
                     /locus_tag="VEL74_02620"
     CDS             2348..2602
                     /locus_tag="VEL74_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HYG61449.1"
                     /translation="MTHIITEPCVGTKDTACVDVCPVDCIYGKNEDWEILYIHPEECI
                     DCGLCVDACPVEAIFPLEEVPDKWKSWIAKNYEHFGMTPP"
     gene            2859..>3034
                     /locus_tag="VEL74_02625"
     CDS             2859..>3034
                     /locus_tag="VEL74_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HYG61450.1"
                     /translation="MDYKDYYETLGVQKNASQDEIQKAYRKLARKFHPDVSKEPGAEL
                     KFKEIGEAYEVLKDP"
BASE COUNT          414 a         1114 c         1054 g          452 t
ORIGIN      
        1 catcggcggc tacggccccc acatcctcgg gcaccgccac ccggccatcg tggcggcgat
       61 ccaggaggcc ctggggcgcg gcacggcgtt cggcgccccc accctcgccg aggtggagct
      121 ggccgagatc ctgaccgaag ccctgccggc attggagatg gtccgcctgg tcaattcggg
      181 caccgaggcc accatgtcgg ccctgcgcct ggcccgggcc gccaccggcc gcaaccgctt
      241 catcaagttc caggggtgct accacggcca cggcgactct ttcctggtcg ccgccggctc
      301 cggcgccgcc accatcggcg tcccctcctc ccccggcgtg ccggcgacgg tgaccgccga
      361 caccctgctc gcgccgtaca acgacctgga ggcggtggag gccctgttcc agtccggaga
      421 tgctggggac gtggccgccg tgttcgtcga gccggtggcc gggaacatgg ggctggtgcc
      481 gccggccccc ggcttcctcg ccgggctgcg cgagctgtgc gaccgccacg gcgcgctgct
      541 ggtcttcgac gaggtgatga ccggcttccg ggtcgcctgg gggggagccc agaacatctt
      601 cgacatccgg ccggacctca ccaccctggg caaggtgatc ggcggcggcc tgccgctcgc
      661 ggcctatggc ggccggcgcg acctgatgcg ccggatcgcc ccggccgggc cggtctacca
      721 ggccgggacg ctgtcgggca acccgctggc ggtggccgcc gggcgggcgg cgctctccat
      781 cctgggccag gacgtggact gcccctacga gcagctcgag gccaccggcg cccgcctcca
      841 ggccgggctc caggaggcgg ccgcgcggca cggcatcccc tgccgggtgg agcgccaggg
      901 ttccatgatc ggcctcttct tcaccgattc gccagtgcgc cgcctggagg acgtcgacgc
      961 ctccgaccgc gagcgcttca agcgcgtctt ccatcgcctg ctcgccgacg gcgtccacct
     1021 gccgccgtcc ccctacgagg cgctgttcct ctccaccgcc cacggcgacg ccgagatcga
     1081 tctcaccctc gaggccttcg accgcgcgct ctcccaggag gcggcgcggt gaggggtacg
     1141 ccatgagccg gcccgacgtc ctcgtgctgg gcggcgggat catcggcctg gcctgtgccc
     1201 gggtcctggt ccgcgaaggc ctctcggtcg aggtggtgga gcgcctgtcg gccggggccg
     1261 aagcctccac cgccgccgcc ggcatgctgg cgccgctctc cgagacgccg gtccccggcc
     1321 cgttcctcga cgcctgccgc gcctcccgcg acctctggcc ctcctgggtg gccgggctcc
     1381 aggaggagag cggcctcgcc atcgactacg acaccggcgg cgccttccat ctcgcactgg
     1441 gagaagagga ggagacgggc ctggaccggc tcgccttggg cgccgccgag atcggcgagc
     1501 cggccgtgga ggtggagccc gccgtcctcg ctcaccgcat ccccggcgtc tcccccgagg
     1561 tccgccgcgc cctccacctg ctcggcgagc accgggtgga caacatccag gcctgcgcgg
     1621 ccctggccgt cgcctgccgc cggctcggag tgatcctgag ctacggtgtc cacatcgagg
     1681 aggtgcggcg ccagggggac ggcgtccggg tgatgggcca cggctacacc cgcgacgccg
     1741 gctgcctggt cctcgcggcc ggcgcctgga gcggcggcgt tggcggcagg gggggattgc
     1801 ccccgctgcc ggtgcggccg gtgcgcgggc agatgatgct gctggccggc atcgactggc
     1861 cctggaaggg gacggtccgc ggcaccccgt tctacgcggt gcgccggggc gccaccggcc
     1921 tgctggtggg ggcgaccgtg gaggaggccg gcttcagcgc tcatacgacg ccggccggca
     1981 tcgagagcct gctcgccttc gtccggcgct atctgccggg cctcgccggc gcacggctcg
     2041 agaccctgtg ggccggcctg cgcccgggca cgcccgacgg cctgccgatc ctcggcccgc
     2101 tgccggagtg gccggtggtc gtggccaccg gccactaccg caatggcatc cttctcgcac
     2161 cctggaccgc ggagcaggtc gccctgctga tcagcgccgg cgcgatcgga agcgccggcg
     2221 cctccaacga agacgatccg ttctccccgt cgcggttcct cgccgaggcg acggtgcgcc
     2281 gctgaggcgc cccttggata taatccggcc acgtagggag aactccggcc ttaggagggg
     2341 cctggtcatg actcacatca ttacggaacc ctgcgtcggc acgaaagaca ccgcgtgtgt
     2401 cgacgtatgc cccgtggatt gcatctacgg gaagaacgag gactgggaga tcctctacat
     2461 ccaccccgag gagtgcatcg actgcggcct gtgcgtcgac gcctgcccgg tcgaagccat
     2521 cttcccgctg gaagaggtcc ccgacaagtg gaagagctgg atcgccaaga actacgagca
     2581 cttcgggatg acccctccct gacgggcggg ccctcctgat cttgcggaaa gaggcgcggg
     2641 cgaccgcgcc ttttctgctt caggagggtg tacctccccc cctctcccca ccttgcggcc
     2701 ccgcctgtca aatcttttct cccccgggaa tggaaaaacg gccccgatcc gttctatgat
     2761 gtgagtgccg agcactcaat ctttcctgcg tgttcggagg tgctgccgtg agatggtctg
     2821 agcaggacgc tcggagaaca gagggaggag aggttccatt ggattacaag gactactacg
     2881 agactctcgg cgtccagaag aacgcctcgc aggacgagat ccagaaggcg tatcggaagc
     2941 tggcccgcaa gttccacccg gacgtcagca aggagcccgg ggccgagctc aagttcaagg
     3001 agatcggcga ggcgtacgag gtgctgaagg accc
//
LOCUS       DASPJI010000099         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_65828, whole genome shotgun sequence.
ACCESSION   DASPJI010000099 DASPJI010000000
VERSION     DASPJI010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65828"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..515
                     /locus_tag="VEL74_02630"
                     /pseudo
     CDS             228..515
                     /locus_tag="VEL74_02630"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
     gene            complement(588..1475)
                     /locus_tag="VEL74_02635"
     CDS             complement(588..1475)
                     /locus_tag="VEL74_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYG61451.1"
                     /translation="MQSRLDFLRSAQSVGLVLSGGCSRCSFQVGAIEVLDELGIRPSL
                     CIGVSGGVWNAAAVVGGVSHRLRRYWRCFIRMPHMDLRNLARQDHSPWVFNEVHRRTF
                     GRYVGVERLRRPDAPSLWAGVTRLRDRAPVFFRAQDFEDPLLPLLASNYLPPFFTHAP
                     RIDGERYADGGLSDNVPYLKAFEEGCDAVVVVTMKGESESLGVYRNPTDIDHQIPEAF
                     RDRLVLIRPRHYLPVRFTDRRWPVLLQTMELGRLRAREVLLGEVHPATDQRGEAGLGH
                     RLFKLVNRSYRFVGGAKVS"
     gene            1577..2158
                     /locus_tag="VEL74_02640"
     CDS             1577..2158
                     /locus_tag="VEL74_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HYG61452.1"
                     /translation="MRRVCVFCGSSTGVRPVHAEAARVMGAALARRGLGLVYGGGNVG
                     LMGIVADAVLERGGEVIGVIPEALMARELGHQGLTELRVVGSMHERKALMAELADAFV
                     ALPGGFGTFEELCEVITWTQLGLHPKACSVLDVDGYYAPLLALFDHAVAEGFIRPQHR
                     ALVIAESDPERLLDRLAAFQPPAVAKWIDRGET"
     gene            complement(2155..>3300)
                     /locus_tag="VEL74_02645"
     CDS             complement(2155..>3300)
                     /locus_tag="VEL74_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYG61453.1"
                     /translation="LERTLKPPTSERLAEIRVPTLLILGSLDVPGIQKVIGLLEKGVP
                     GAKKVVIQGAGHMVNMEKPEEHDRALLGFLDSLPKGSAGFTNKASAEGYVPADNGIHL
                     HYRKVGSGPETLVIPWVTLRQADDLSRLAEGRTLIFYSPRGRLRSDTVDPSQVSFEND
                     LADLETVRRHFGLEKMALLGWSHYGLMTAVYAARHPERVTRVVQMTPGAPRRDSYREQ
                     MDTAIRERVDGAAWSALQKRRESGELAGKPAEACRAQRQVVTAAYLGNPSMLSRVPLD
                     DCDLTNEQGEPQQRWWGALFASMGAWDVRSDLRGLRVPRLVVAGEKDFIPMAASREWV
                     AGHPEARLLVLPGVGHFPLVEAPDRLLSAVEAFLDGRWPEGAEAISG"
BASE COUNT          504 a         1142 c         1183 g          471 t
ORIGIN      
        1 gtggcgacga cgtggtagac ggggtccgcg gtggccactg tggctgtagc gatgaggccg
       61 aggacccaga gggccgcggc ggcgaaggtc gagagcttgc caggcatggt ctcctccaat
      121 ccgaaatcga gcgagccatc ctagcgcagg ttgcgcggcc tcccggccac gggccagaac
      181 ggggaatgat cgagctgatc tgcacccgca cgtgaggtac ctgccgcaag gcggtcgcct
      241 tgctcgacga gaagggaatc ccctaccact accgggagta cacccgggag cccctgtcgg
      301 agaaagagat ccgcgacgtg ctgcgccggc tgggcctgac cgcccgggcc gtcctgcgca
      361 agaatgacat ggggctgacc ggcgacgagc ccgaggccga gctcatcgcc gccatggccc
      421 gtcagcccac gctcctccag cgccccatcg gcgtgctggg agaccgggcg gtggtgggcc
      481 ggccgccgga gaggctgctg gagctgacgg gttagaggcg aaggcttcca ggtgagggga
      541 accggcccgg cgccgcgctc cccgtggtgg cgcccgttgc ttgagcgcta cgacaccttc
      601 gcgccgccga cgaaccggta ggagcggttg actagcttga agagccggtg gcccaggccg
      661 gcctcgccgc gctgatcggt ggcggggtgc acctcgccca ggagcacctc gcgggcgcgc
      721 aggcggccga gctccatcgt ctggaggagc accggccagc ggcggtcggt gaacctcacc
      781 ggcaggtagt ggcgcggacg gatcagcacg aggcggtcgc ggaaggcctc cgggatctgg
      841 tggtcgatgt ccgtgggatt gcgatagacg cccaggctct cgctctcccc cttcatggtc
      901 accacgacca cggcgtcgca cccctcctcg aaggccttga ggtaggggac gttgtcggag
      961 aggccgccgt cggcgtagcg ctcgccgtcg atccgcggcg cgtgggtgaa gaaggggggc
     1021 aggtagttgg aggcgaggag cggcagcagc gggtcctcga aatcctgcgc cctgaagaag
     1081 accggagcgc ggtcacgcag acgcgtgacg cccgcccaga gggacggcgc gtccggccgt
     1141 cggagccgct ccacccccac gtagcgaccg aaggtccggc ggtgcacctc gttgaaaacc
     1201 cagggcgagt ggtcctgccg ggccaggttc cggaggtcca tgtgcggcat ccggatgaag
     1261 cagcgccagt atcggcgcag ccggtggctc actccgccca ctaccgccgc cgcgttccac
     1321 acgccgccgg agacgccgat gcagagggag gggcggatgc ccagctcgtc caggacctcg
     1381 atcgcgccaa cctggaagga gcagcgggag cagccgccgg aaaggacgag cccgacactt
     1441 tgggcggagc ggaggaaatc gagccgggac tgcatacgac gctgcccgga cgcccgggcg
     1501 gcgcttattg tagccgctcc gcgaacggcg tgcacggcct ctgttagagt ccggcgcgga
     1561 aaagtggagg ttggtcatgc ggcgcgtctg tgtcttctgt ggttcgagca ccggggtccg
     1621 tccggtccat gccgaggccg cccgggtgat gggggcggcc ctggcccggc gcgggctggg
     1681 gctcgtctat ggcggcggca acgtcgggct catggggatc gtggccgacg ccgtgctgga
     1741 gcggggcggc gaggtgatcg gcgtgatccc ggaggcgctg atggcccgcg agctcggcca
     1801 ccaggggctc accgagctgc gggtagtggg ctcgatgcac gaacgcaagg cgctgatggc
     1861 cgagctggcc gacgccttcg tcgctctgcc cggcggcttc ggcaccttcg aggagctgtg
     1921 tgaggtgatc acctggacgc agctcggcct ccaccccaaa gcctgctccg tccttgatgt
     1981 cgacggctac tacgcccccc tgctcgccct gttcgatcac gcggtggccg aagggttcat
     2041 ccgcccccag caccgggcgc tggtcatcgc ggagagcgac cccgagcgcc tgctcgaccg
     2101 gctggcggcg ttccagccgc ccgcggtcgc gaagtggatc gatcggggcg agacttagcc
     2161 cgagatggcc tccgccccct cgggccagcg gccatccaag aaggcttcga cggcggagag
     2221 caggcgatcc ggcgcctcca cgagcgggaa gtggccgacg cccggcagga ccagcaggcg
     2281 cgcctcggga tggcccgcca cccactcccg cgaggcggcc atggggatga agtccttctc
     2341 ccccgccacc accagccgcg gcacccgcag cccgcgcaga tcgctccgca cgtcccaggc
     2401 tcccatcgag gcgaagaggg ctccccacca gcgctgctgc ggctcgccct gctcgttggt
     2461 caggtcgcag tcgtcgaggg ggacgcgcga cagcatcgag gggttcccga gataggcggc
     2521 tgtaaccacc tgcctctggg ctcggcacgc ctcggccggc ttgcccgcga gctcccccga
     2581 ctcccggcgc ttctgcagcg cgctccaggc cgcgccgtcc acccgctcac gaatcgccgt
     2641 atccatctgc tcgcggtagg agtcacgccg cggtgcgccc ggcgtcatct gcaccacgcg
     2701 cgtgacccgc tccggatgcc gcgccgcgta gaccgccgtc atcagcccgt aatgggacca
     2761 gccgaggagg gccatcttct cgagcccgaa gtggcgccgc accgtctcca gatccgccag
     2821 atcgttctcg aaggagacct gggacgggtc cacggtgtca gagcggagcc gcccgcgcgg
     2881 cgaatagaag atcagcgtcc gcccctcggc gaggcgcgag aggtcgtccg cctggcggag
     2941 cgtcacccag gggatcacca gcgtctcggg gccgctcccc acctttctat agtggagatg
     3001 aatcccgttg tctgccggga cgtagccctc cgccgacgcc ttgttcgtga aaccggcgct
     3061 ccccttgggc aaggagtcca ggaaacccag cagtgcgcgg tcgtgctcct ccggcttctc
     3121 catgttgacc atgtggccgg cgccctggat caccaccttc ttcgcaccgg gaacgccctt
     3181 ctccaggagg ccgatcactt tctggatacc cgggacgtcg aggctgccca ggatgaggag
     3241 cgtcggcacc cggatctccg ccaggcgctc ggacgtcggg ggcttgaggg tgcgctcgag
//
LOCUS       DASPJI010000100        10411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_807356, whole genome shotgun sequence.
ACCESSION   DASPJI010000100 DASPJI010000000
VERSION     DASPJI010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10411)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10411
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807356"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VEL74_02650"
     CDS             complement(<1..447)
                     /locus_tag="VEL74_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61454.1"
                     /translation="MSEREQGPRPPRRQRRAAERKRGKAAGKAASGAALTLAALAAGQ
                     TAEAATFSVTNLNDSGAGSLRQAIEDANAAAGADVVAFQAGLTGTITLTTGQLEITDS
                     VDIQGPGAAVISVSGNNASRVFYLYNGSAELDVTISGLTVTGGNDIA"
     gene            523..3426
                     /locus_tag="VEL74_02655"
     CDS             523..3426
                     /locus_tag="VEL74_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03032 family protein"
                     /protein_id="HYG61455.1"
                     /translation="MRLDTELLQLPLLFDADRLAAEVAAIPEEHWRPHPQGHPGNSAL
                     PLVAAGGDPANDATKGPMRPAPHLARCPYLRQVLAAFGSPIGRTRLMRLDGNAEATPH
                     SDTNYYWQQRVRIHVPVITRPEVLFLCGERSVHMAAGEAWIFDTWKTHNVLNPNPTRR
                     IHLVADTVGSAAFWDLAARAAAEPRLVPFDPDHDPELDFETVNQPVVMSPWEQEVLLT
                     SFLQDLPPSPLATELESELAPFRQDWRSLWATHGESPTGWPAYRALLDRLQTRLARYE
                     GRLRLANGSEAVEILRQMVIRPALNPDLSSRSQGAVVPPSQHRELMSAPFDRPIFIVG
                     SPRSGTSLLFETLAQSPDLWTIGGESHAVIEGIGALHPAHRGWDSNRLPAEDASPETV
                     ALLTRSFLTHLRDRDGNAPPSGATALRLLEKTPKNSLRLPFLAAAFPDARFLYLYRDP
                     RETISSMLDAWRSGRFVTYPDLPGWPGPPWSLLLVPGWRELAGRDLAEIVARQWLLAT
                     SRLLDDLEALPPGRWCVASYDRLVAEPQAEIERVCEFIGVRWDRPLAAPLPLSRHTLT
                     SPEPEKWRHNGEELKPVMPLITEVAQRARDLFANPPVIRPTRRPLADAPRTVADPNAF
                     RSVHTGAMPQLLEQIGSSLLVSTYQSGRLIAVRSDGGALNTHFRAFPSPMGIAVRPGH
                     LVFGTQRHVLEYRDQPEVGRKLKPPGKHDACFLPRKSHVTGDIRVHELAFTSDGLCIV
                     NTRFSCLAALDGENSFVPRWRPPFVSALAPEDRCHLNGLAIVDGRPRFVTALGATDSP
                     QGWREGKAAGGVLIDVPSGEIVARGLSMPHSPRWSAGRLWVLESGKGEVGVVDLAAGR
                     VETVARLDGFTRGLAFAGPYAFVGLSQVRESNIFGGLPLSERVREKLCGVWVLDVRSG
                     RTVGFLRFEGLVQEIFDIQILPGIRYPEIAEPDSDLVAGSFTLPQEAMPEVAL"
     gene            complement(4081..4362)
                     /locus_tag="VEL74_02660"
     CDS             complement(4081..4362)
                     /locus_tag="VEL74_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61456.1"
                     /translation="MEGQLRHRYRMKEKGLRIGARLRKGGVELDTMGRPKEEVRRLLD
                     LLPDEASYEDIQYEIYVRQAVDRGIEAADRGELVEQEEIERRMARWLGR"
     gene            complement(5091..5549)
                     /locus_tag="VEL74_02665"
     CDS             complement(5091..5549)
                     /locus_tag="VEL74_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG61457.1"
                     /translation="MRFWDSSALLPLLVREPTSQRLEDLLRLDTEIALWWGTPVECAS
                     ALARVHRSEDLRTSDLRKAQALLSHLHARAYEIQPVEEVRARAQRLLAVHRLRAADAL
                     QLAAALVWCRERPQGFAFVCLDDRLRGAAALEGFTVMPYSEEVHEEDFED"
     gene            complement(5546..5824)
                     /locus_tag="VEL74_02670"
     CDS             complement(5546..5824)
                     /locus_tag="VEL74_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYG61458.1"
                     /translation="MRSAGIAELKASLSETLARVKAGEEVLVTEHGRPIAKIVPLSAQ
                     TATDELVRSGVLRVPERPLDEEFWQLPRPQDSSALVRGALLKERREGR"
     gene            5965..7143
                     /locus_tag="VEL74_02675"
     CDS             5965..7143
                     /locus_tag="VEL74_02675"
                     /inference="COORDINATES: protein motif:HMM:NF021793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG61459.1"
                     /translation="MNTPSLVRRLPAIAVLLTLLGAFPAAAARPASVLDDAAFRADVK
                     VGLDHLYGMDYAAATEVFSRIEARHPGHPVGPFLRAMVPWWTLLLDPEDTRHDAEFLG
                     AMDEVLRRSEARIRRNPNDMDALFFRTGAHAFRARIYAFRKQWMKAGNDGRRALSNLR
                     KLQKKDPENVDLLFGVGLFDYLVDVVPRQHKYLRPVAVLFPKGNRARGLAELERAGKE
                     GRFVQTEAHYALYQVYMTFEKDYRKAMYEVAWLRARHPENSLFKVAEGRVYVRQGRWA
                     EASLIFQDVAERQVAGERGYSGALAQEALYWLARSEMSAGRYAGALQYLDRLDYLAKE
                     RKYDLYFQAAGRLRRGMAYDALGQRTDATRCYRDVLAMEIKGDVRDRAEEFLEKPYAG
                     "
     gene            complement(7384..8295)
                     /locus_tag="VEL74_02680"
     CDS             complement(7384..8295)
                     /locus_tag="VEL74_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HYG61460.1"
                     /translation="MRLCYFIQNHLAPSQVARLVRTLRRTRPDAFILVCHDELAGYAA
                     VEEVRRAVDADVFTAREPARRGDYSLIQPYFDALEWLAARGVAYDWIVYLSGQDYPTQ
                     PLRRLEERLATGGYDGYLRFWDVRDAENPWGRRHQGIRRYFYQYTEAPAWTAPALRLL
                     KALNGVQSWIHVQMTYGPRIGLRRRPPFGPELTVYTGKAWTILSRACAEHVAESGRSQ
                     GELVRWFQRTLVPDEAVVQTLLVNSGRFRLCNDDLRYMDFTGSRDGRPRTLTERDLPV
                     LTGGEYYFARKFERDSRVLDLLDERIG"
     gene            complement(8382..9425)
                     /locus_tag="VEL74_02685"
     CDS             complement(8382..9425)
                     /locus_tag="VEL74_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HYG61461.1"
                     /translation="MTKKLGWISLMAFLALLIPGTVLMAQEGQPERDPLLKMMEEGWK
                     PVAPGVLQRSFEDNKVETFAVGPEGIKWTIKQLRARMAFLQKENQAYPSAETRQAIAS
                     YRKQIAKFQKELRSAKSESLESAIEKTGCSVSYSSYANAAPLTSTAGVTSNSSANFTN
                     TCGYSATVYAEAYARATKNGVTDTFTQTDGPRSGANVSASATATIQGDSNCLSTSTSY
                     ATYSSITLSFSASNSVCPVENPAVTISGTTWESFSSYGCRSKTWTTAVSGGTAPYTYQ
                     WYLDGTPVGTGSSYSRSVCSYDDPGYTLSVTVTDSGSRTGSDTHSVSVSYFEPPPTCW
                     QGGMQVICNDQIQ"
     gene            9749..>10411
                     /locus_tag="VEL74_02690"
     CDS             9749..>10411
                     /locus_tag="VEL74_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1"
                     /protein_id="HYG61462.1"
                     /translation="MKYRSGWLVVTPLCVLLLGASPPTPPPSQRLVHEGIAIDLTVEP
                     LSAERTGGLREGERARVRFRVSDAHTGAPVAGLYPGAWMDRRKDADGSATKNETDKET
                     CRKKVQGFVGGSFLNQPALDLNVYYVLAMNQDATLSVVDPLFGFGSSKLRGMVFLRSP
                     GEDWALAADGRSLFVSMPDSDRVAVAETEAWKVTAELETGPRPRRVVLQPDGQYLWVA
                     YEG"
BASE COUNT         1603 a         3749 c         3400 g         1659 t
ORIGIN      
        1 ggcgatgtca ttgccgccgg tcaccgtcag gcccgagatg gtgacgtcca gctccgccga
       61 gccgttgtag aggtagaaga cgcgcgaggc gttgttgccg ctgacggaga tcaccgccgc
      121 gcccggaccc tggatgtcca ccgagtcggt gatctcgagc tggccggtgg tcagggtgat
      181 ggtgccggtg agccccgcct ggaacgccac gacgtccgcg ccggccgcgg cgttggcgtc
      241 ctcgatggcc tggcgcaggc tgccggcgcc gctgtcgttc agattggtga cgctgaacgt
      301 cgccgcctcg gccgtctgtc cggccgccag cgccgccagc gtcagcgcgg cgcccgaggc
      361 ggccttgccc gccgccttgc cacgcttcct ctccgccgcc cgccgctgcc ggcgtggcgg
      421 ccgtggaccc tgctctctct cgctcatacg ttccccccca ggaaccggaa tggatcaaag
      481 acgcgcttag tataaggtcg cacctttgga tctggaacgc ccatgcgact cgatacagag
      541 cttcttcaac tgcccctgct cttcgacgcg gaccgcctcg ccgcggaggt ggccgcgatc
      601 cccgaggagc actggcgtcc ccatccccag ggacatccgg gcaactcggc cctgccgctg
      661 gtcgccgccg gaggcgaccc ggcgaacgac gccaccaagg ggccgatgcg gccggcgccc
      721 cacctcgccc gctgccccta cctgcgccag gtgctggccg ccttcggctc ccccatcggc
      781 cgcacccgac tcatgcgcct ggacggcaac gccgaggcga cgccgcactc cgacaccaac
      841 tactactggc agcagcgggt gcgcatccac gtgccggtga tcacccggcc ggaggtcctc
      901 ttcctctgcg gcgagcgctc ggtccacatg gcggccggcg aggcctggat cttcgacacc
      961 tggaagaccc acaacgtcct caaccccaac cccacgcggc gcatccacct ggtggcggac
     1021 accgtgggct ccgccgcctt ctgggacctg gccgcccgcg ccgccgccga gccgcgcctc
     1081 gtccccttcg accccgacca cgatccggag ctggacttcg agacggtcaa ccagccggtg
     1141 gtgatgtccc cctgggagca ggaggttctc ctcacctcct tcctccagga cctccccccg
     1201 tctcctctgg ccacggagct cgaatccgag ctcgccccgt tccgccagga ctggcgctcc
     1261 ctctgggcca ctcacggcga gtcgcccacg ggctggcccg cctaccgcgc cctcctcgac
     1321 cgtctccaga cccgcctggc ccgctacgag ggtcgcctgc gcctggccaa cggctcggag
     1381 gcggtggaga tcctccgcca gatggtgatc cggccggcgc tgaatccgga tctcagctcg
     1441 aggtcgcagg gggcagtggt gcctccctcc cagcatcgcg agctgatgtc agcccccttc
     1501 gaccgcccca tcttcatcgt cggctccccc cgctcgggca ccagcttgct gttcgagacc
     1561 ctcgcccagt cgccggacct ctggacgatc ggcggcgaga gccacgcggt gatcgagggc
     1621 atcggcgcgc tccaccccgc ccaccggggc tgggactcca accgcctgcc cgccgaggac
     1681 gcctccccgg agaccgtcgc cctcctcacc cggagcttcc tcacccatct gcgcgaccgg
     1741 gacggcaacg ctccaccgtc aggcgccaca gccctccgcc tcctcgagaa gacgcccaag
     1801 aactcgctgc gcctcccctt cctggcggcg gcgttccccg acgcccgctt cctctatctc
     1861 tatcgggacc cccgcgagac catctccagc atgctggacg cctggcggtc cggccgcttc
     1921 gtcacctatc cggacctccc ggggtggccg ggtcctccct ggtccctgct cctggtgccg
     1981 gggtggcggg agctggcggg ccgcgacctg gcagagatcg tggcccgcca gtggctcctc
     2041 gccacctcgc ggctgctgga cgacctcgaa gccctgccgc ccgggcgctg gtgcgtcgcc
     2101 tcctacgacc gcctcgtggc cgagccgcag gccgagatcg agcgcgtctg cgagttcatc
     2161 ggcgtccgct gggaccggcc gctggcggcg ccgctccccc tgtcgcgcca cacgctcacc
     2221 tcgccggagc cggagaagtg gcgccacaac ggcgaggagc tcaagccggt catgccgctg
     2281 atcaccgagg tggcgcagcg ggcgcgggac ctcttcgcca acccgccggt catccgcccc
     2341 acccggcgcc ctctggccga tgccccgagg accgtcgcgg accccaacgc cttccggagc
     2401 gtccacaccg gcgccatgcc ccagttgctg gagcagatcg gctcgtcgct cctcgtctcc
     2461 acctaccaga gcgggcggct gatcgccgtc cgctccgacg gcggcgccct caacacccat
     2521 ttccgcgcct tccccagccc catggggatc gccgtccgcc ccgggcacct cgtcttcggc
     2581 acccagcgcc acgtcctgga gtaccgcgac cagcccgagg tgggccgcaa gctcaagccg
     2641 ccgggcaagc acgacgcctg cttcctcccc cgcaagagcc acgtcaccgg ggacatccgg
     2701 gtccacgagc tggccttcac cagcgacgga ctgtgcatcg tcaacacccg tttctcctgc
     2761 ctcgcggcgc tggacggcga gaacagcttc gtgccgcgct ggcgtccccc cttcgtctcg
     2821 gccctcgccc cggaggaccg ctgccacctg aacggcctgg cgatcgtcga cggccggccg
     2881 cgcttcgtca cggcgctggg agcgaccgac tccccccagg ggtggcgcga gggcaaggcg
     2941 gccggcggcg tgctgatcga cgtcccgagc ggcgagatcg tcgcccgggg actctccatg
     3001 ccgcactccc cgcgctggtc cgcgggccgg ctctgggtgc tggagtccgg caagggggag
     3061 gtcggcgtgg tggacctcgc ggccgggagg gtcgagaccg tggcccggct cgacggcttc
     3121 acccgcggcc tggccttcgc tggcccctac gcctttgtag gattgtcaca ggtgcgggag
     3181 agcaacattt tcggcggcct ccccctgtcc gagcgggtac gggagaagct ctgcggcgtc
     3241 tgggtgctcg acgtccggag cggccggacg gtgggattcc tccgcttcga ggggctcgtg
     3301 caggagatct tcgacatcca gatcctcccc ggcatccgct acccggagat cgccgagccg
     3361 gactcggacc tggtggccgg ttccttcacc ctccctcagg aggcgatgcc ggaggtggct
     3421 ctgtgaggga tctgagggta gccgcccgag ggacggctac cctcgagctg gagcattact
     3481 gcgcggggca gagcgctacg atgaggaagg cgttacaggt cgtacgctcg ccgtcgagct
     3541 cgcagggtac atggccgggg accctctggc agaaatcgca gccgcaggcg cgagcggcgg
     3601 atgccggcag ggcggcggcg gcgacaatca gagtgagggc caggatccga acaaggctga
     3661 gaagtttcat aggagctcct ttcgtcaggt cagccgttca aatcccagga ttcatgcaaa
     3721 tgcctggccc gaggagccgg ccagagttga tggcgagtag gtctagattg cagccatcct
     3781 gctcgccatc atgtgtccca ctcccggacc attgctgtga gatcccgtgc accatgaatg
     3841 aaagcgatga tctggacctc gcggcctaca cccgatacag catccggtag ctccagacga
     3901 gaagctcccg aatgtccggg tcacccacct ccggcactat acgcccacgg ttcgcgaggt
     3961 gccgaagcga tctcgccgca tcgcgagcct ctcggatcag cgcggcggcg tagcgagggg
     4021 agtcttgggc gatgtattcg gcggcggcct cgagattcga ccaggctggc tcggtccact
     4081 ttacctgcca agccaccttg ccatccgccg ttcgatctct tcctgctcca ccaactctcc
     4141 ccgatccgcg gcctcgatgc cgcggtcaac ggcttgacgg acgtaaatct cgtactggat
     4201 gtcctcatag gacgcctcgt ccgggaggag gtccagcagg cggcgaactt cttcctttgg
     4261 tctacccatc gtgtccaatt ctactccccc tttccgcagg cgagccccaa tcctgagccc
     4321 cttctccttc atcctgtacc gatgcctcag ttgtccttcc aagagaatat accctgaagc
     4381 gagcaaccac tggcacgttc cagggcctct ctcgctccac ccgccaccca ggggggaggc
     4441 accaccgccc cctcgcgtca ggccgtttac ctgacattat ccctgcggcc ccgaacacta
     4501 tccccggtgc ccgggacacc atccctccat ccgggattac taccccgctc tccggatcac
     4561 tatccccgcc tcggggacac gatccccgcg cgcgggttca ttcggggagg cgagaggaac
     4621 attccggacg cttcccggaa cgccgtaccc gcttctgcat cgttcccgat cgggggatga
     4681 cggtccgcga cgcgcggatt gtgcccctcc cccggaggct tgtgacccca cgagactgcc
     4741 caagccccgg gcacgacgcg ccgccggccg cgcccgagac ctggtccatc gattgtcaaa
     4801 gaactccctg gctgaacccc agagttcagc ccacagggcc gaatcgtcat tcatcccgcc
     4861 tgggatgtca acaagccatt gacaccttgg tctactccca tgcagaattg gctgcacgga
     4921 gaactcatcc cgctacccgg cagagtcctc aaggacgttc cttgacaact gaatcgcaac
     4981 aggggcggcg ccttgtccaa gccgcgccct gtgaggtccc gctcgtggga ggcggaggga
     5041 ctggcctccg cctccctgag ccagacagtc ctctctcgaa ggggctcgcc tcagtcctcg
     5101 aagtcctctt cgtggacctc ttccgagtag ggcatgaccg tgaagccctc cagagcggcg
     5161 gcccccctca gacggtcgtc gaggcagacg aaggcgaagc cctgcgggcg ctcacggcac
     5221 cagacgaggg cggcggcgag ctgcagggcg tccgcggcgc gcagccggtg gacggccagc
     5281 agacgctggg cgcgggcgcg cacctcctcg acgggctgga tctcgtaggc ccgggcgtgg
     5341 aggtggctca gaagggcttg ggccttgcgc aggtcggagg tccggagatc ctcgctacgg
     5401 tggacgcgag ccagggcgct ggcacattcc accggtgtcc cccaccagag tgcgatctcc
     5461 gtgtccaggc ggagcagatc ctccaatctt tggctggtgg gctcccggac gaggaggggc
     5521 aggagagccg agctgtccca gaacctcaac ggccttcccg acgctctttg aggagcgccc
     5581 ctcgcaccag ggcggacgag tcctggggcc gcgggagctg ccagaattct tcgtcgagcg
     5641 gccgctccgg aacacgcagg accccagacc gtaccaattc gtcggtggcg gtctgggcgg
     5701 agaggggaac gatcttggcg atgggcctgc cgtgctcggt caccaggacc tcctcaccgg
     5761 ccttgacccg ggccagcgtc tcggaaagag aggctttcag ctcagcgatg ccggcgctac
     5821 gcatgatgga gactacccta tccccgcaga catgaccagt caagtcatac ttgcctgaaa
     5881 tctcccggcc cacggctttg ttttttggag gcaggcggct cccccgggag gaggggccgc
     5941 ctcttctata aggaagccaa ggcagtgaat acgccttctc ttgtccgccg gctgccggcc
     6001 atcgccgtcc tgctgaccct gctcggggcc ttccccgccg ccgcggcgcg cccggcctcg
     6061 gtcctcgacg acgccgcctt ccgggcggac gtcaaggtcg gcctcgacca cctctacggc
     6121 atggattacg ccgccgccac cgaggtcttc tcgcggatcg aggcgcgcca ccccggccat
     6181 cccgtagggc cgttcctgcg cgccatggtc ccctggtgga cgctgctcct cgatccggaa
     6241 gacacccgcc atgatgccga gttcctcggt gccatggacg aggtgctccg ccgctccgaa
     6301 gcgcgtatcc gtcgcaatcc gaacgacatg gacgccctct tcttccggac cggggcccat
     6361 gccttccggg cgcggatcta tgccttccgc aagcagtgga tgaaggcggg caacgacggc
     6421 cgccgggcgc tgtccaacct gcgcaagctg cagaagaagg acccggagaa cgtcgacctc
     6481 ctgttcgggg tcgggctctt cgactacctg gtggacgtcg tccccaggca gcacaagtac
     6541 ctccggccgg tcgcggtgct cttcccgaag ggcaaccggg cccgcgggct cgccgagcta
     6601 gaacgggccg gcaaggaggg gcgcttcgtc cagaccgagg cccactacgc cctctatcag
     6661 gtctacatga ccttcgagaa ggactaccgg aaggctatgt acgaggtggc ctggctacgc
     6721 gcccggcacc cggagaacag cctgttcaag gtggccgagg ggcgggtcta cgtccgccag
     6781 gggcgctggg ccgaggcgag cctgatcttc caggacgtgg ccgagcggca ggtggcgggg
     6841 gagcgcggct acagcggcgc gctggcccag gaggcgctct actggctggc gcgcagcgag
     6901 atgtcggcgg gccgctacgc cggcgccctg cagtacctcg accgtctcga ctacctggcg
     6961 aaggagcgca agtacgacct ctacttccag gccgccggcc ggctgcgccg gggcatggcc
     7021 tacgacgccc tgggccagcg gaccgacgcg acccgctgct accgtgacgt cctcgcgatg
     7081 gagatcaagg gcgacgtccg cgaccgcgcc gaggagttcc tcgagaagcc gtacgcgggc
     7141 tgagcgcggc cgatatcgag cctggcgcgg cctgcgacag gccgcgccaa gccgaccggc
     7201 ggcgccgcgg tgcccacctc aggccccgcg gcgcccacct tcggcatcgc ccggcccgtc
     7261 acgggccgca ccgggccggc cctcggcagg gccaagcccg tcacgcgcct caaggagccc
     7321 acgatcggca gcgccgagcc cgtcacgcgc ctcgcccggc cggagaggcg caacgcggag
     7381 ccttcagccg atccgctcgt ccaacaggtc gaggacccgg gagtcccgct cgaacttcct
     7441 ggcgaagtag tactcgcccc cggtgaggac gggcaggtcg cgctcggtga gggtgcgggg
     7501 ccggccatcc ctgctgcccg tgaagtccat gtagcgcagg tcgtcgttgc acagacggaa
     7561 gcggccgctg ttgacgagca gcgtctggac gacggcttcg tccggcacga gggtgcgctg
     7621 gaaccagcgg accagctcgc cctggctccg gccgctctcg gcgacgtgct cggcgcaggc
     7681 ccggctcagg atcgtccagg ccttgccggt gtagacggtg agctccggac cgaacggcgg
     7741 ccgccggcgc aggccgatcc gcggcccgta ggtcatctgc acgtggatcc atgactgcac
     7801 gccgttgaga gccttgagca gccgcagggc cggcgccgtc cacgccggcg cctcggtgta
     7861 ctggtagaag tagcggcgga tgccctggtg gcggcgaccc caggggttct ccgcgtcccg
     7921 cacgtcccag aagcgcagat agccgtcgta gccgccggtg gcgagccgct cctccaggcg
     7981 ccgcagcggc tgggtgggat agtcctgccc cgacaggtag acgatccagt cgtaggcgac
     8041 cccgcgcgcc gccagccatt ccaacgcgtc gaagtacggc tggatcagcg agtagtcccc
     8101 ccgccgcgcc ggctcccgcg ccgtgaagac gtcggcgtcg acggcccgcc gcacctcctc
     8161 caccgcggca tagccggcga gctcgtcgtg acagacgagg atgaaggcgt ccggccgtgt
     8221 gcgccggagc gtccgcacca gccgtgccac ctgggagggc gcgagatggt tctgaatgaa
     8281 gtaacagagg cgcatgggag agcgggctca gcgaggattg taacgtgcaa gcccgaaagg
     8341 cagcggccgg cggggccgtg tgcggccccg ccggtgggta gctactgaat ctgatcgttg
     8401 cagatgacct gcatgccacc ctgccagcag gtcggcggag gctcgaagta cgagacggac
     8461 acggaatgag tgtccgagcc ggtccggctc ccgctgtcgg tgacggtgac cgaaagcgta
     8521 taaccgggat cgtcgtacga gcagacggag cgcgagtagg agctacccgt tccgacaggg
     8581 gtgccatcga gataccactg gtaggtgtag ggggcggtgc cgccagaaac agcggtggtc
     8641 catgttttcg acctgcaacc gtaggacgag aaggactccc aggtcgtgcc gctgatggtc
     8701 accgcgggat tctcgacggg gcagaccgag ttcgaggcgc tgaagctcag ggtgatgctg
     8761 ctgtaagtgg catacgaagt cgaggtcgac aggcagttgc tgtcgccctg gatggtagcg
     8821 gtggcactgg cgctaacgtt ggccccggaa cgcgggccgt ccgtctgggt gaaggtgtcg
     8881 gtcactccgt tcttcgtagc gcgagcgtag gcctcggcgt agacggtggc tgagtagccg
     8941 caggtgttgg tgaagttggc cgaggaattt gacgtgactc cggccgtaga ggtcaagggt
     9001 gctgcgttgg catacgagct gtacgagacg ctgcaaccgg tcttttcgat cgcgctctct
     9061 agcgactcgg acttggccga ccgcagctct ttctggaact tggcgatctg cttgcggtag
     9121 ctcgcgatcg cctgacgggt ctccgccgac ggataggcct ggttctcctt ctgcaggaag
     9181 gccatccggg cccggagctg cttgatcgtc cacttgattc cctcgggccc taccgcgaac
     9241 gtctcgacct tgttgtcctc gaagctgcgc tgcaggacgc cgggggccac cggcttccac
     9301 ccctcctcca tcatcttgag gagcggatcc ctctcaggct gcccctcttg agccatgagc
     9361 accgtcccgg ggatcagcag tgcgaggaag gccatcagtg agatccaacc aagctttttc
     9421 gtcatgaaca ctccttaacg ctacacgtgg gcgtgtcgcc cagccacggc ggccggacgt
     9481 tctgacagag acgaggccgg gcaaaccctg aggtttcccg gctccgcagt gaaatcacct
     9541 acaccaactt gcgaaggaat ctatcttctg gactggcgcg agtcaacagt tgccaatata
     9601 gacaggattc gatggacagg gcccgccccg ggggccaggg gcatcgacat cgtccgccgc
     9661 ctgtcgccgg agaggccggg cgtctacttc gctctttaca tttgattcgc aactcgaata
     9721 gagtcccgcc ccgcaggaga agtcattgat gaagtatcga agtggctggc tcgttgtcac
     9781 ccccctctgt gtcctcttgc tcggggcctc cccccccacc ccgccgccct cgcagcgcct
     9841 cgtccacgaa gggatcgcca tcgaccttac ggtcgagccg ctcagcgccg agcgtaccgg
     9901 cggcctgcgt gagggcgagc gtgcccgggt gcggttccgc gtctccgatg cccacactgg
     9961 cgcccccgtg gccgggctct accccggcgc ctggatggac cggcggaagg acgccgacgg
    10021 gtccgcgacc aagaacgaga ccgacaagga gacctgccgc aagaaggtgc agggcttcgt
    10081 cggcggctcg ttcctcaacc agccggccct ggacctcaac gtctactacg tgctggcgat
    10141 gaaccaggac gccaccctct cggtggtcga cccgctgttc ggcttcggca gctcgaagct
    10201 gcggggcatg gtcttcctcc ggagccccgg ggaggactgg gccctggcgg cggacggccg
    10261 cagcctcttc gtctccatgc ccgactcgga ccgggtggcc gtggccgaga ccgaggcctg
    10321 gaaggtgacg gccgagctcg aaaccggccc gcggccgcgg cgggtggtcc tccagcccga
    10381 cggccagtac ctctgggtgg cctacgaggg g
//
LOCUS       DASPJI010000101         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_65895, whole genome shotgun sequence.
ACCESSION   DASPJI010000101 DASPJI010000000
VERSION     DASPJI010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65895"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2151)
                     /locus_tag="VEL74_02695"
     CDS             complement(<1..>2151)
                     /locus_tag="VEL74_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61463.1"
                     /translation="DRVGLPELFAAQAGQRPDSLAVLYGDHGLSFAELDRLSDRLARR
                     LGASGVGLESRVGLCLERSPELIVGMLGILKAGGAYVPLDPDYPAERLALLIEDAGVV
                     ALVVTATTERAAGIPAGGLPVLLLDGEEGREGAGGRERKGSVRPVAPDGANLAYVIYT
                     SGSTGRPKGVEVTHRAVSRLVLNTDYVRLGPADRVAQAATASFDAATYEIWGPLLHGG
                     CVVGITKETLLDPGALADALRRYGVTSLFVTTALFHQVARESPTAFATLDSLLFGGEA
                     IDPRRVREVLAGGPPRRLLHVYGPTESTTFATWQKVTEVPEGATTVPIGQPIANTRLF
                     VLDPLHQPVPLGVAGELCLAGDGLARGYTARPALTAANWIPDPFGALFGESGERLYRT
                     GDLVRLRPDGAVEFLGRIDSQVKVRGFRIEPGEIEACLAEHTAVLEAVVVAREDAPGE
                     RRLVAYVVGDAGYAPANDPAERPGYVAQWESLFDDIYRRGANETDPTFNIVGWDSTYT
                     GQPLPREEMVEWLDDTVERIRSLAPRRVLELGCGTGMLLFRLARSAEIYVGTDISRRV
                     LDEVEAHLATWAERPPVELQQRRADELSGFAPGSFDTVILNSVVQYFPSADYLAEVIA
                     GAVALVRPGGSIFIGDLRSLPLLDAFHTSVELERAEADLPLDRLRQRIQVRRLHENEL
                     VVAPEFFAALKQTLPGVGRVEIHPKRGRAHNELTG"
BASE COUNT          328 a          773 c          765 g          285 t
ORIGIN      
        1 cccggtcagc tcgttgtgcg cccgcccccg cttggggtgg atctccacgc gcccaactcc
       61 cggcagggtc tgcttcaggg cggcgaaaaa ctcgggggcg acgaccagct cgttctcgtg
      121 caggcgccgc acctggatcc gctggcgcag gcggtccaac ggcaggtcgg cctccgcccg
      181 ttccagctcc accgaggtgt ggaacgcgtc gagcaggggg aggctgcgta agtcgccgat
      241 gaagatcgag ccaccgggcc gtaccagcgc caccgcgccc gcgatcacct ccgcaaggta
      301 gtcggcgctc gggaagtact gcaccaccga gttgaggatc accgtgtcga aggagccggg
      361 cgcgaagccc gagagctcgt cggcccgccg ctgctgcagc tcgaccggcg ggcgttcggc
      421 ccaggtggcg aggtgggcct cgacctcgtc gagcacgcgg cgggagatgt cggtgcctac
      481 atagatctcg gcggaccgcg cgagccggaa gaggagcatg ccggtgccac agccgagctc
      541 gagcacccgg cgcggcgcga gcgagcggat gcgctccacc gtgtcgtcga gccactcgac
      601 catctcctcg cgcggcagcg gctggcccgt ataggtgctg tcccagccga cgatgttgaa
      661 cgtaggatcg gtctcgttcg ccccgcgccg gtagatgtcg tcgaacagcg actcccattg
      721 ggcgacatac ccgggccgct ccgcggggtc gttggccggc gcatacccgg cgtctcccac
      781 cacgtacgcg accaggcgcc gctccccggg cgcgtcttcg cgcgccacga ccaccgcttc
      841 cagcaccgcc gtgtgctcgg ccaggcaggc ctcgatctcc cccggttcga tgcggaagcc
      901 gcgcaccttg acctgggagt cgatccgccc caggaactcg accgcgccgt ccggccgcag
      961 gcgcaccaga tcgccggtgc ggtagagccg ctcgccgctt tcaccaaaga gggcgccaaa
     1021 gggatcgggg atccaattcg cggcggtgag agcgggccgg gccgtatagc cgcgcgccaa
     1081 gccgtcgccc gccagacaca gctcgccggc gacgcccagg gggaccggct ggtggagcgg
     1141 atcgagcacg aagagccgcg tgttggcgat cggctggccg atggggaccg tggtcgcccc
     1201 ttccggcacc tcggtcacct tctgccaggt agcgaaggtc gtgctctcgg tcgggccata
     1261 gacgtggagc aggcggcgcg gcggtccccc ggccaggacc tcccgcaccc ggcggggatc
     1321 gatcgcttcg ccgccgaaga ggagcgaatc gagcgtcgcg aaggccgtgg ggctctcccg
     1381 cgccacttgg tggaacaggg ccgtggtgac gaaaagggag gtgacgccat agcgccgcag
     1441 ggcgtcggcg agggcccccg gatcgaggag cgtctccttg gtgatcccca cgacacagcc
     1501 gccgtggagc aacggccccc agatctcata ggtggcggcg tcgaacgagg ccgttgcggc
     1561 ctgggccaca cggtcggcgg ggccgagacg cacgtaatcg gtgttcagca ccaggcggga
     1621 gaccgcccgg tgcgtcacct cgaccccctt ggggcggccg gtggagccgg aggtatagat
     1681 gacgtaggcg aggttcgctc cgtccggcgc cacgggccgg acggagcctt tcctttctct
     1741 tccccctgcc ccttccctac cctcctcgcc gtcgagcagg aggaccggca ggccgcctgc
     1801 gggaatcccc gccgccctct ccgtcgtcgc cgtcacgacc agggcgacga cgccggcatc
     1861 ctcgatcagg agggccagcc gctcggccgg gtagtcggga tcgagaggga cgtacgcgcc
     1921 gccggccttg aggatcccca gcatgccgac gatcagctcg ggggagcgct ccaggcagag
     1981 ccccacgcgg ctctcgagcc cgacgcccga cgcgcccaga cggcgggcca gccggtcgga
     2041 gagacggtcc agctcggcga agctcaagcc gtgatcgccg tagagcacgg ccagggagtc
     2101 cggccgctgc cccgcctgtg ccgcgaaaag ctcgggcaga cccacgcggt c
//
LOCUS       DASPJI010000102         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_177541, whole genome shotgun sequence.
ACCESSION   DASPJI010000102 DASPJI010000000
VERSION     DASPJI010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177541"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1474)
                     /locus_tag="VEL74_02700"
     CDS             complement(<1..1474)
                     /locus_tag="VEL74_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61464.1"
                     /translation="MSHRRTRLPLLAALLAIGALALATTATAAVGSWSPLGPDGESIS
                     ALAADPTNADLVYAATPTSVFRSSDGGDSWTFAGSGLGSTSAPGVRALALSGAVVIAG
                     TTWEGLWRSLDEGRTWRAITAGFPGRPHVNALVVDPRRPERVWLAGWEGVFVSEDRGE
                     TWAVRNGNLPPAGAVESLVIDPANGQLYARTTSELFTSSDEGASWSRLSCLGRRCGSV
                     ALDPADPDTLFTISGGLLRSRDGGTVWEKLRAPRGGLFAFLGFHDGRLFAYSRSYARG
                     LAADRLYWSADQGASWVPAAGQPAARSIGVMASSGGTLYLGSAGIASLGGVFRSGDGG
                     EHWEAASSGLSGRWIESIAVDPQRPGVLYAQASDHLLVSEDDGASWRLSLAAPNVLVF
                     GGGDLLVDPNLRGRVWSTTGDQLWTSNDGGRRWLAVRRVGAGIGALAADPRTRGGVWA
                     GGRNGLFHSPTGRALRKVRPVRNENLGVVDIAVAPDDPRVV"
     gene            1829..>2094
                     /locus_tag="VEL74_02705"
     CDS             1829..>2094
                     /locus_tag="VEL74_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYG61465.1"
                     /translation="MGLILSALLSGCGKGEEPRQAEPAANQEAPRATRGYIVISIDTL
                     RADHLGCYGYPRPTSPFLDSLAERATLFEEAYAQYPSTLVSHMS"
BASE COUNT          306 a          737 c          765 g          286 t
ORIGIN      
        1 aaacgacccg cggatcatcc ggcgccacgg cgatgtccac cacaccgagg ttctcgttcc
       61 gcaccggccg caccttcctc aaggcgcggc cggtggggct gtggaacagg ccgttccggc
      121 cgccggccca gacgccgccg cgggtacggg gatcggcggc gagcgctccg atgccggcgc
      181 cgacccggcg caccgccagc cagcggcgcc ctccgtcgtt gctggtccag agctggtcgc
      241 cggtcgtcga ccagacgcgc ccgcggagat tggggtcgac gaggaggtcg ccgccgccga
      301 agaccagcac gttgggggcg gcgaggctca ggcgccagct cgcgccgtcg tcctcgctga
      361 cgaggaggtg gtcggaggcc tgcgcgtaca gcacgcccgg ccgttgcggg tcgacggcga
      421 tggactcgat ccacctgccc gagagacccg agctggcggc ctcccagtgc tcgccgccgt
      481 cgccgctacg gaagacgccg ccgaggctgg cgatgcccgc cgagccgagg tacagggtgc
      541 cgccgctcga cgccatcacc ccgatcgagc gggccgcggg ctgcccggcg gcgggtaccc
      601 aggacgcgcc ctggtcggcg ctccagtaga gccggtctgc ggccaggccg cgggcatacg
      661 agcggctgta ggcgaacagc cgtccgtcgt ggaagccgag gaaggcgaag aggccgcccc
      721 tgggcgcccg cagcttctcc cagaccgtgc cgccgtcgcg gctgcgcagc aggccgccgg
      781 agatggtgaa gagggtgtcg gggtccgccg gatcgagggc gacgctgccg cagcggcggc
      841 cgaggcagct cagccgcgac caactcgccc cctcgtcgct gctggtgaac agctcgctcg
      901 tcgtgcgtgc gtagagctgg ccgttggcgg ggtcgatgac cagcgactcg acggcgccag
      961 cgggcggcag gttgccgttg cgcaccgccc aggtctcgcc gcggtcctcg ctgacgaaca
     1021 ccccctccca acccgccagc cagacgcgct cgggccgccg cgggtcgacg acgagggcat
     1081 tgacgtgcgg ccggcccggg aagccggcgg tgatggcgcg ccaggtgcgg ccctcgtcga
     1141 gcgagcgcca gaggccctcc caggtggtgc cggcaattac caccgcgcca ctgagcgcca
     1201 gggcgcggac gccgggggcg ctggtgctgc cgaggccgct gccggcgaat gtccaactat
     1261 cgccgccgtc gctgctgcgg aacacggagg tcggggtggc agcgtagacg agatcggcgt
     1321 tggtggggtc cgcggcgagc gcggagatgg actcgccgtc ggggccgagc ggagaccagg
     1381 agccgacggc cgccgtcgcc gtcgtggcga gcgccagcgc gccgatggcc agcaaggcgg
     1441 cgagaagggg gagacgggtg cggcggtggc tcataggata ccctccggtc tgcgcgatag
     1501 cccaacttca accatctagt ttagctgaca tgggagtgcc tggtacctcc ctccagcacc
     1561 ttcggcgatg ctcggcgagg tttggccgga gatggtgacc cggaaacccg tcccctcttc
     1621 gtggtccgtc tgggagggca cccgcgggag ctttcgcggg gccgcgcgcc ctccggcgtg
     1681 tcctgctacg accttgatag cccgatcata gaagagatga aagcagaaaa agagcgtcct
     1741 gcggcgcctt gatcgggttt tctttactcg tacgctagga ttcccccgca tgcgtcgaca
     1801 ccgaggaggg ccgtgggccc gagtggccat gggcctgatc ctctccgccc tcctctccgg
     1861 ttgcggcaag ggcgaggagc cacggcaggc tgagcccgcg gccaatcaag aagcgcctcg
     1921 ggccacccgg ggctacatcg tcatctccat cgacacgctg cgagccgatc acctggggtg
     1981 ctacggctat ccccggccga ccagcccgtt cctcgattcg ctggccgagc gcgcgacgct
     2041 gttcgaggag gcctacgcgc agtacccgag cactctggtc tcccatatgt cgat
//
LOCUS       DASPJI010000103        12210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_295843, whole genome shotgun sequence.
ACCESSION   DASPJI010000103 DASPJI010000000
VERSION     DASPJI010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12210)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12210
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295843"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VEL74_02710"
     CDS             complement(<1..453)
                     /locus_tag="VEL74_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HYG61466.1"
                     /translation="MLERLFHVRAAGSTPGRELLGGLTTFLTMAYILAVNPVFLTAAG
                     IPLEGAIVATCLASAFATLLMAFAANYPIALAPGMGMNAFFAYGVCVGGGVPWQTALG
                     LVFWAGILFVLLTVTGARRLLVSAVPPVIHFAGAVGIGLFIAFIGLPHG"
     gene            1649..2416
                     /gene="pyrF"
                     /locus_tag="VEL74_02715"
     CDS             1649..2416
                     /gene="pyrF"
                     /locus_tag="VEL74_02715"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HYG61467.1"
                     /translation="MAEPNSELNADPILLSTKTIPPEERLIFALDVPGADEAKRLVTT
                     LREAVEFYKVGLELFLDAGFELVDWILEQEKKVFLDLKLFDVPETVRRAVRQISRRPV
                     TFTTVHAGSRSILEAAAAEKGEVKILAVTVLTSLDKGDLNELGADASDIDVERLVLSR
                     ARRALEAGCDGVIASGLEAAPLRRELGGELHIVTPGIRPAENRPADDQKRVVTVEQAL
                     LNGADYIVVGRPIRDAADPYQAALDIQGSIEGLFVSG"
     gene            complement(2406..3920)
                     /locus_tag="VEL74_02720"
     CDS             complement(2406..3920)
                     /locus_tag="VEL74_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYG61468.1"
                     /translation="MIPGWTRRAFLKGAALSLPALASPRLGAAQEAATVAPQAAQGLA
                     RGASPKKVIVVGAGLAGLAAAWELVQAGHDVTVLEAQLRPGGRVYTLRSPFADGLYAE
                     AGAIAFSDTRTLRRYLEAFQLASEPFRQTLPAWFHLRGQRFEAPNWPYELTAEEKKLG
                     IGGMLPKYFGPGVTLGDPTAPGFRLAAHESLDRMTVAELLRKQGASDEAIALLGATMT
                     FGYGWSTGSALHRLISDVALFFAGNQKNLVLTGGSDVLPRAFAKALQERILYGAPVVE
                     VVQEPAKVRAVYRQGGQAGAERSLEADYLICTVPSPVLRRVRFTPELPAPKRRIFERL
                     EFTPVTRIYLQTRRRLWEDTKHAGGAFTDLPVQQVTEHPLAPSAVKAQGPRGLLEAHI
                     KGPEARRIGAMPLEAQLALAAESLEKVHPGYREHFEGGASVDWAADPWAGGGYAEWKP
                     GQMTEWLPELVKPEGRVHFGGEHTSVLSRTLEGALESGMRAAREVHEAAAGPSR"
     gene            complement(3917..5623)
                     /locus_tag="VEL74_02725"
     CDS             complement(3917..5623)
                     /locus_tag="VEL74_02725"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYG61469.1"
                     /translation="MVSRIFKSRSIQAGAGASGLLACLWALLLIVAAPVQAGTEAAAK
                     AGIANLRMNAPQSPMPDGRLGRAPETLTADKTGKLLVAAWESMHGTCGAPFGGACTPP
                     KKPGIVGVGYSADGGKTWTDAGAPYIDGSKVMSSGHPWIDRGGADGETFFLSTRARTV
                     EATPRDGTPGGSGQVGLVVYRGRFKDGALTWTDEHVFRPRKQGDLLRSQSLTAAKDGS
                     GRVYLTLTTLHGICGQAGRSAGQIEVFRSADSGKTWEGPVIVGADDTLVTEDPKDPKC
                     GSQGTIQLSSTAALGPKGEVYLIWQFGPAGKVVGNEFVMNPALAVRFAVSTDGGRTFS
                     APRDIATANTMRVNLPVGYSKTSINDYPRIAVAGGSGGGKHRGRIYVTYTSAVGGVGG
                     SGDQQSHTSSQVFLVWSDDQGKTWSRPAPLAPAIHETGVKRIWPTVAVRDDGKVDVVY
                     LESQERNVTADPNDLECKAAIIFNNTRESKVSSMVVIYWVQSADGGASFGAPVRVTSE
                     TSNWCQAKYDIEGTQFANFGDYLGIVTAGNRAFTVWTDSRNGVPDAYFAELPAGAPGA
                     KK"
     gene            5894..>12210
                     /locus_tag="VEL74_02730"
     CDS             5894..>12210
                     /locus_tag="VEL74_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61470.1"
                     /translation="MEQNEISSRLAGLSPAKRALLERRMRGEAQGPKAAGIGRRAGTG
                     PAPLSFSQQRLWFLDQLEPGSPTYNLRVLLRLAGPLHREALARSFQEVVRRHEVLRTA
                     FQVADGVPVQAVLPWLELALPAIDLTSLPEREREGESLRQALALADRSFDLAAPPLVR
                     LALLDLGAGVHHLLVVMHHIVSDGWSTGVLVREVGALYNAFMADAPSPLAELPIQYGD
                     FAVWQRGWLAGGVKEQHLGYWRERLAGVSAGLDLPTDRPRPAVQSFRGATHRFEVGTG
                     LTEALTALARREGMTPFMVLAAGLFSLLSRLSGEADLTLGTPIANRNRPEVEGLIGFF
                     VNTLVLRAEAGRPRDILALLHHVREVTLGAYAHQDLPFEELVAELHPDRDLSRTPLFQ
                     VALAYENAPPLGSALAGVELSPVELPVGSAKFDLAFILRGDGETLAGTLEYATDLYDG
                     ATAARLCDHWLRLLETAVADPALEPAAIALLSPAERHQLLREWNDTRAATEGEALLHQ
                     LFEARVDLQPGAEAAAWQGESLTYQQLEERSNRLANLLQRLGAGPESPVAVWMERSLD
                     MVTALLGASKAGATYLPIDAGWPSDRAEAVLAGTGAAVVLTRQAHLPAVRELQWRLPR
                     LGDAVCLDVDAPAPPPEPLDAAAVRGLFDVVADRAADWVNDWVAAAGFVSSYTGEAFA
                     EADVLEYRDRVLALAEPWLTTGGADATGKRVLEIGAGSGLILWELAPRVGRTGRYVGV
                     DPSPRTQERNRERAAREGHANVELPTGFAHEIADLAETPPESFDLVILASTVQFFPGL
                     SYLEHVLELALSRLAPGGALVIADVMDPRRREDFRRSLAAWAAAHPGSAAASRAGDRT
                     GEGRLYVDEDLFTDLFGGAAAVLHREQGFANELGFRYDVVIRKAGAASKAEPAQRRKR
                     VWTGWHLAAASAARPLPVASPVDIAYVIHTSGSTGAPKGIVVQHRPAVELCRWINSTF
                     HVGPGDRLLFITSPAFDLSVYDVFGVLAAGGAVHVASEEALRDPEELVRLLVEETVTI
                     WDSAPAALQQLAPLLPAGGERPEARLRLVLLSGDWIPVPLPDVVRAAFPGARVISLGG
                     ATEATVWSNWFPIGEVDPRWPSIPYGRPITNARYHVLDRALQPCAIGVPGDLYIGGDC
                     LCVGYARLPEMTAERFVPDPFAAEPGARLYGTGDRARYGRDGNLEFLGRVDQQVKVRG
                     YRIELEEIEAVLARHPGVREAAVVAREDIPGDKRLVGYFVPMAEPGPSAAELRASLQQ
                     VMPDYMVPWTFVALERMPITANGKLDRKALPAPREVAEAGAAYEAPRTGLELAVAGVW
                     QEVLRLPRVGLGDDFFELGGSSLLATQVISRIRQSCGVELPLRALFEARTLAEMAKRV
                     ESLQEGPALPATAAPPLTPAPRTGPLPLSFAQQRLWFLDQLVPGSPLYNIPGALRLTG
                     ALDPALLGGVLAEVARRHEVLRTTFRAEGGEPVQVIAAEPRLPLPVVDLAGLPAARRR
                     EERERRTAAEARLPFDLATGPLLRAALLRLAPGDHVVLITLHHIVADAWSMEVFSREI
                     GALYGAWSREPSRRRLPSPLPELALQYADYAVWQRRWLAGDALEAELEYWRRQLAGAP
                     RVLELPADRPRPAVQSYRGSWRPLALPAGLGAELTGRAQRRGATLFMALLGAFATVLG
                     RYAAREDVVVGSPVAGRDRPEIEGLIGFFVNTLVLRVDLAGDPGFDGLLDRVRAMALD
                     AFAHQSLPFERLVDELAPVRDPSHSPLFQVAFAFQTAPREPLRLRGLTMEPLHDDSGT
                     AKVDLLLSLTEAGGEVSGLWEYSTDLFDEVTIDRWSGHLASVLAAVATEAGGEGRRLS
                     ELPLLAPAERHQLLSEWNDTRDAVDGEATLDRLFVTTAGQWPDRVAVSFEDHSVTYRE
                     LAARAGRLARTLRAMGVEPEVLVGICAEESIERTVGVVGTVLAGGAFLPLDPAHPAER
                     LELMAEDARIRVLLTQEHLRPSLPAADAAVVCLDDYVRHRQEPVHRASFPGSLPGPWG
                     PVPDNLSYVIYTSGSTGRPNGVQVSHRSAVHLLRQAARHTLADEGSRVLQLVSFSFDA
                     SVLETWLAFSTGGALCVIGQETRLSGAALAET"
BASE COUNT         1526 a         4435 c         4512 g         1737 t
ORIGIN      
        1 gccgtgcggc aggccgatga aggcgatgaa caggccgatg ccgacggcgc cggcgaagtg
       61 gatcaccggc ggcacggcgc tcaccagcag acgccgggcg ccggtgacgg tgaggagcac
      121 gaacaggatg ccggcccaga acaccagccc gagcgccgtc tgccagggga cgccgccgcc
      181 gacgcagacg ccgtaggcga agaaggcgtt catccccatc cccggcgcca gggcgatcgg
      241 gtagttggcg gcgaaggcca tgagcagggt ggcgaacgcc gacgccaggc aggtggcgac
      301 gatcgccccc tccaggggga tgccggcggc ggtcaggaag actgggttga ccgccaggat
      361 gtaggccatg gtgaggaagg tggtgagccc cccgaggagc tcccggccgg gcgtcgagcc
      421 cgcggcgcgc acgtggaaca ggcgttcgag catggctggg ggtagtttac ggcagggccg
      481 gcccgcaggc ctcgcccccc gtccgggacg ctcggcaaga ttccgggccg tctggaatcc
      541 atccgattgg attggacgcg atccggttcg accggatgaa gtccggagcg tccgggagct
      601 tcccgagcgc tccggatcgc gtcccggccg cgcggaagct tcccggagcc cccggatggc
      661 atccgcggcg cccggacgga atccgcggcg ttcggatgga gtccacggcg cctggacgcg
      721 atccgaggcg gtgagagcct cgcctgcatc aggaagaggt cggtgcatga gggcggtggt
      781 gcctcccccc ctgggcgggt gggcggtagg gagcggggga ggtcaggagg ggggtttttc
      841 tccgctcccg gaacccgtcg agcctgacgg gcgtcacctt tctggaagtc tgacgccgtc
      901 ggcgagacgt ccgccgcctc gcgcggcccg cctatccata gaaggaagca gcgccttctc
      961 cgccgtcgtg ggggaggcgc ttttctcttg ccggagcttc cgacggcgga ccggaagctc
     1021 cggcggcccg ccgaaggata cggcggcgcg tcggagaccc tgacagctcg ccggaagctc
     1081 tgagagcccg ccggtagctt ccgggagccc gccgaaggct acgatggcgc gccggagacc
     1141 ccggcagcgt gccggaaact ccgacagctc gctggaagct tccaacgacc cgtcagagat
     1201 tgcgacggcg cgtcggaaac tccgacagcc cgtcggaagc ttcggcggcg cgtcgaagac
     1261 tccgatagtg tgccggaagc ttccgatggc gcgttggaaa ctctgacagc gcgtcgaaag
     1321 cttccaacgg cctgccgaag agtacgacgg cgcccgccag ccctttcagg gctccagatg
     1381 tttgtggggg cccctacctg ccgttgaaac ggcaggcttt atgcccgcgc cgcttcgcgg
     1441 ctaaaacccc cctcctgacc tcccccactc cctaccgccc accctcccag ggggggagga
     1501 acccccgccc gaggtaccac caccccctca tcctctgcct cttcttgcgt ccgtctcccc
     1561 ctctcccggg gagggggcag ggggagggga tgagacagcg aggcgggaga gggggccggg
     1621 gggtgaggcc tgtgctagct tctcccccat ggctgaaccg aacagcgaac tgaacgccga
     1681 tccgatcctg ctgtccacca agaccatccc ccctgaagag cggctcatct tcgccctaga
     1741 cgtgcccggg gccgacgagg cgaagcggct ggtgaccacg ctgcgcgagg cggtcgagtt
     1801 ctacaaggtc ggcctggagc tgttcctgga tgccggcttc gagctggtcg actggatcct
     1861 cgagcaggag aagaaggtct tcctcgactt gaagctcttc gacgtgccgg agacggtgcg
     1921 ccgggcggtg cgccagatca gccggcgccc ggtcaccttc accaccgtgc acgccggcag
     1981 ccggagcatc ctggaggccg ccgccgcgga gaagggggag gtcaagatcc tggcggtgac
     2041 ggtgctcacc agcctggaca agggggacct gaacgagctg ggcgccgatg ccagcgacat
     2101 tgacgtggag cggctcgtcc tctcccgcgc gcggcgcgcg ctggaggccg gctgcgacgg
     2161 cgtcatcgcg tctggcctcg aggcggcgcc gctgcgccgc gagctggggg gggagctgca
     2221 catcgtcacg cccggcatcc ggccggcgga gaaccggccc gcggacgacc agaagcgggt
     2281 ggtcaccgtc gagcaggcgc tgctcaacgg cgccgactac atcgtcgtcg gccggccgat
     2341 ccgcgacgcc gccgacccct accaggccgc cctggacatc caggggagca tcgaggggct
     2401 gttcgtcagc ggctaggacc ggcagccgcc tcgtgcacct cgcgggcggc gcgcattccg
     2461 gactccagcg ccccttcgag ggtgcgcgag agcaccgagg tgtgctcgcc gccgaagtgc
     2521 acccgcccct cgggcttgac gagctcgggc agccactcgg tcatctgccc cggcttccac
     2581 tcggcgtagc cgccgcccgc ccaggggtcg gcggcccagt ccaccgaggc gcccccctcg
     2641 aagtgctcgc ggtacccggg gtggaccttc tccaggctct ccgccgcgag ggcgagttgc
     2701 gcctccaggg gcatggcgcc gatgcgccgc gcctcggggc ccttgatgtg cgcctccagc
     2761 agcccgcgcg gcccctgcgc cttgaccgcg gacggggcga gcggatgctc ggtcacctgc
     2821 tgcaccggca ggtcggtgaa ggcgccgccg gcgtgcttcg tgtcctccca gaggcgccgc
     2881 cgcgtctgca ggtagatccg ggtcaccggc gtgaactcca ggcgctcgaa gatccggcgc
     2941 ttcggcgccg gcagctcggg ggtgaagcgc acccggcgca gcaccgggct ggggacggtg
     3001 cagatcaggt agtccgcctc cagcgaccgc tcggcgcccg cctgcccccc ttgccggtag
     3061 accgcccgca ccttggccgg ctcctggacg acctccacca ccggcgcgcc gtagaggatc
     3121 cgctcctgga gcgccttggc gaaagcgcgc ggcaggacgt cggagccgcc ggtcagcacc
     3181 aggttcttct ggttgccggc gaagaacagg gcgacgtccg agatcagccg gtggagcgcc
     3241 gagccggtgg accagccgta gccgaaggtc atggtggcgc cgagcagggc gatggcctcg
     3301 tccgacgccc cctgcttgcg cagcagctcc gcgacggtca tccggtcgag gctctcgtgc
     3361 gccgccagcc ggaagccggg ggcggtcggg tcgccgaggg tgacgccggg gccgaagtac
     3421 ttggggagca tgccgccgat gccgagcttc ttctcctcgg cggtgagctc gtagggccag
     3481 ttgggcgcct cgaagcgctg gccgcgcagg tggaaccagg cgggcagcgt ctgccggaac
     3541 ggctcgctgg cgagctggaa cgcctccagg tagcggcgga gggtgcgggt gtcggagaag
     3601 gcgatggctc cggcctcggc gtagaggccg tcggcgaacg ggctgcgcag ggtgtagacg
     3661 cggccgccgg ggcggagctg ggcttcgagc acggtgacgt cgtggccggc ctggaccagc
     3721 tcccaggcgg cggcgagccc ggccaggccg gcgccgacca cgatcacctt cttcgggctg
     3781 gcgccgcgcg cgagtccctg ggctgcctgg ggagccactg tcgccgcttc ctgcgccgct
     3841 cccaggcggg gggaggcgag ggcgggcagc gacagcgccg cgcccttcag aaaggcgcgg
     3901 cgcgtccagc cgggaatcac ttcttggctc cgggcgcgcc ggccggcagc tccgcgaagt
     3961 aggcgtccgg cacgccgttc cggctgtcgg tccagacggt gaaggcgcga ttgcccgcgg
     4021 tgacgatccc gaggtagtcg ccgaagttcg cgaactgcgt cccctcgatg tcgtacttgg
     4081 cctggcacca gttgctggtc tcggaggtga cgcggaccgg agcgccgaag ctggcgccgc
     4141 cgtcggccga ctgcacccag tagatgacca ccatcgagct gaccttgctc tcgcgcgtgt
     4201 tgttgaagat gatggcggcc ttgcactcga ggtcgttcgg gtcggcggtg acgttccgct
     4261 cctggctctc caggtagacg acgtcgacct tgccgtcgtc gcgcacggcg acggtcggcc
     4321 agatccgctt gacgccggtc tcgtggatcg ccggggcgag cggcgccggc cggctccagg
     4381 tcttgccctg gtcgtcggac cagaccagga agacctgcga cgaggtgtgg ctctgctggt
     4441 cgccgctgcc gccgaccccg ccgaccgcgc tggtgtaggt gacgtagatc cggccgcggt
     4501 gcttgccgcc gccgctgccg ccggccaccg cgatccgcgg gtagtcgttg atcgacgtct
     4561 tgctgtagcc gaccggcagg ttgacgcgca tcgtgttcgc ggtggcgatg tcgcgcggcg
     4621 cgctgaaggt gcggccgccg tcggtcgaga cggcgaagcg gacggccagc gccgggttca
     4681 tcacgaactc gttgccgacg accttgccgg cggggccgaa ctgccagatc aggtagacct
     4741 cgcccttggg gcccagcgcc gccgtcgagg agagctggat cgtcccctgg ctgccgcact
     4801 tggggtcctt ggggtcctcg gtgaccaggg tgtcgtccgc gccgacgatg accggaccct
     4861 cccaggtctt gccgctgtcg gcgctgcgga acacctcgat ctgcccggcc gagcggccgg
     4921 cctggccgca gatcccgtgc agggtggtga gcgtcaggta gacccggccg ctgccgtcct
     4981 tggcggcggt gaggctctgg ctgcgcagca ggtccccctg cttgcgcggc cggaagacgt
     5041 gctcgtcggt ccaggtgagg gcgccgtcct tgaagcggcc gcggtagacc acgagcccga
     5101 cctggccgga gccgccgggg gtgccgtcgc gcggcgtcgc ctcgacggtg cgcgcgcggg
     5161 tggagaggaa gaaggtctcg ccgtcggcgc cgccgcggtc gatccagggg tggccggagc
     5221 tcatcacctt gctgccgtcg atgtacggcg cgccggcgtc ggtccaggtc ttgccgccgt
     5281 cggccgagta gccgacgccg acgatgcccg gctttttggg cggcgtgcag gcgccgccga
     5341 acggcgcgcc gcaggtgccg tgcatcgact cccaggcggc gaccaggagc ttcccggtct
     5401 tgtccgccgt cagggtctcc ggcgcgcggc cgagccgccc gtcgggcatc ggcgactgcg
     5461 gcgcgttcat ccgcaggttc gcgatgcccg ccttggcggc cgcctctgtc ccggcctgga
     5521 cgggagccgc cactatcagg agaagggccc agaggcaggc aaggagtccg gaggccccgg
     5581 ctccggcttg gatgctccgc gacttgaaga ttcttgaaac cattgacttc atctcctgat
     5641 ggcaattgct cgcaattttc ttaatatcgg ccgttcggtg tatttctcgc ggccgagctg
     5701 ggcaacttta cgagaaagcg cggttccgaa gtatactttg gctccagcac tctctgcagc
     5761 tggtggtcgt gccgtcacct tctatgcggg tgagtcggcc ccaatatttc gaattttcga
     5821 agccgaggcg tcggtgccgc ggtggcgccg gtcggccggg agtgtccccg gtatctcagt
     5881 caggtaggcc agcatggaac agaatgagat ctcatcgcgc ctggccgggc tctctcccgc
     5941 caagcgcgcg ctgctcgagc gccggatgcg cggcgaggct cagggtccca aggccgccgg
     6001 catcggccgc cgggccggga cgggccccgc ccccctctcg ttctcgcagc agcgcctctg
     6061 gttcctggat cagctcgagc ccggctcgcc gacctacaac ctgcgggtgc tgctgcggct
     6121 cgcggggccg ctgcaccgcg aggccctcgc ccgcagcttc caggaggtgg tgcgccggca
     6181 cgaggtgctg cgcacggcgt tccaggtggc ggacggggtg ccggtgcagg ccgtgctgcc
     6241 gtggctggag ctggcgctgc ccgcgatcga cctcacctcg ctgccggagc gcgagcggga
     6301 gggggagtcg ctgcgccagg cgctggcgct ggccgaccgc agcttcgacc tcgccgcgcc
     6361 gccgctggtg cggctggcgc tgctcgacct gggcgccggc gtgcatcacc tgctggtggt
     6421 catgcaccac atcgtctcgg acggctggtc caccggcgtg ctggtgcgcg aggtgggggc
     6481 gctctacaac gcgttcatgg ccgacgcgcc gtcgccgttg gcggagctgc ccatccagta
     6541 cggcgacttc gcggtctggc agcgcggctg gctcgccggc ggggtgaagg agcagcacct
     6601 cggctactgg cgcgagcgcc tggccggggt ctcggcgggg ctcgacctgc ccaccgaccg
     6661 gccgcggccg gcggtgcagt ccttccgcgg cgccacgcac cggttcgagg tcggcaccgg
     6721 gctgaccgag gcgttgaccg ccctggcgcg gcgcgagggg atgaccccct tcatggtgct
     6781 cgccgccggg ctcttctccc tgctctcccg gctgagcggc gaggccgacc tcaccctcgg
     6841 cacgccgatc gccaaccgca accgccccga ggtcgagggg ctgatcggct tcttcgtcaa
     6901 cacgctggtg ctgcgcgccg aagccggccg cccgcgcgac atcctcgccc tgctgcacca
     6961 tgtgcgcgag gtcacgctcg gcgcctacgc gcaccaggac ctgccgttcg aggaattggt
     7021 ggcggagctg catccggacc gcgacctgtc gcgcacgccg ctcttccagg tggcgctggc
     7081 gtacgagaac gcgccgccgc tgggctcggc cctcgccggc gtcgagctca gcccggtgga
     7141 gctgccggtg ggcagcgcca agttcgacct cgccttcatc ctgcgcggcg acggcgagac
     7201 cctggccggc acgctcgagt acgccaccga cctctatgac ggcgccacgg cggcccgcct
     7261 gtgcgatcac tggctgcgcc tgctggagac ggccgtcgcc gatccggccc tggagccggc
     7321 ggccatcgcg ctgctgtcgc cggcggagcg ccaccagctg ctgcgcgagt ggaacgacac
     7381 ccgggccgct acggagggcg aggccctcct gcaccagctc ttcgaggcgc gcgtggacct
     7441 ccagcccggc gccgaggccg ccgcctggca gggggagtcg ctgacctacc agcagctcga
     7501 ggagcggtcc aaccggttgg cgaacctcct ccagcgcctg ggcgccggtc cggagtcgcc
     7561 ggtggcggtc tggatggagc gctcgctcga catggtgacc gccctgctgg gggcgtccaa
     7621 ggccggggcc acctacctgc ccatcgacgc cggctggccg tccgaccgcg ccgaggcggt
     7681 gctcgccggc accggcgccg ccgtcgtcct gacccgccag gcccacctgc cggcggtgcg
     7741 ggagctgcag tggcggctgc cgcgcctggg cgatgccgtc tgcctggacg tcgacgcgcc
     7801 ggcgccgccg cccgagccgc tcgacgccgc ggcggtgcgc ggcctctttg acgtggtcgc
     7861 cgaccgggcc gccgactggg tgaacgattg ggtcgcggcg gccggtttcg tcagcagcta
     7921 caccggcgag gccttcgccg aggccgacgt cctcgaatac cgcgaccggg tgctggccct
     7981 ggccgagccc tggctcacga ccggcggcgc ggacgccacg ggcaagcggg tgctcgagat
     8041 cggcgccggc tccggcctga tcctgtggga gctggcgccg cgcgtcggcc gcaccggccg
     8101 ctacgtcggc gtcgacccgt cgccgcgcac gcaggagcgg aaccgcgagc gcgccgcccg
     8161 cgaggggcac gccaacgtcg agctgcccac cggcttcgcg cacgagatcg cggacctggc
     8221 cgagacgccg ccggagtcgt tcgacctggt gatcctcgcc tcgaccgtgc agttcttccc
     8281 cggcctgagc tatctcgagc acgtcctcga gctggcgctg tcgcgcctgg cgccgggcgg
     8341 cgcgctggtg atcgccgacg tcatggaccc gcggcggcgg gaggatttcc ggcgctcgct
     8401 ggcggcctgg gccgccgccc accccggcag cgccgccgcg tcccgtgccg gtgaccgcac
     8461 cggcgagggg cggctgtacg tcgacgagga cctgttcacc gacctgttcg gcggcgcggc
     8521 ggccgtcctc caccgcgagc agggcttcgc caacgagctc ggcttccggt acgacgtggt
     8581 gatccgcaag gccggcgccg cctcgaaggc cgagcccgcg cagcgccgca agcgggtgtg
     8641 gacgggctgg cacctggccg ccgcctccgc ggcgcgcccg ctccccgtgg cgtcgccggt
     8701 ggacatcgcc tacgtcatcc acacctcggg ctcgaccggc gcccccaagg ggatcgtcgt
     8761 ccagcaccgg ccggccgtgg agctctgccg gtggatcaac tccaccttcc acgtggggcc
     8821 gggggaccgg ctgctgttca tcacctcgcc ggccttcgac ctgtcggtct acgacgtctt
     8881 cggcgtgctg gcggccggcg gcgcggtgca cgtcgcctcc gaggaggcgc tgcgcgaccc
     8941 ggaggagctg gtgcgcctgc tcgtcgagga gacggtgacg atctgggact ccgcccccgc
     9001 cgcgctgcag cagctcgcgc cgctgctccc ggccggcggg gagcgcccgg aggcgcgcct
     9061 gcgcctggtg ctgctgtcgg gcgactggat cccggtgccg ctgcccgacg tcgtgcgggc
     9121 ggccttcccc ggcgcgcggg tgatcagcct cggcggcgcc accgaggcga cggtctggtc
     9181 caactggttc ccgatcggcg aggtcgaccc gcgctggccg agcatcccct acggccggcc
     9241 gatcaccaac gcccggtacc acgtgctcga ccgcgcgctc cagccgtgcg ccatcggcgt
     9301 tcccggcgac ctctacatcg gcggcgactg cctgtgcgtc ggctacgccc ggctgccgga
     9361 gatgaccgcc gagcggttcg tccccgaccc cttcgcggcc gagccggggg cgcgcctgta
     9421 cggcacgggc gaccgggccc gctacggccg cgacggcaac ctcgagttcc tcggccgcgt
     9481 cgaccagcag gtcaaggtgc gcggctaccg catcgagctc gaggagatcg aagcggtgct
     9541 cgcccgccac cccggagtgc gcgaggccgc cgtggtggcg cgcgaggaca tccccggcga
     9601 caagcggctg gtcggctact tcgtgcccat ggccgagccc gggccgtccg cggccgagct
     9661 gcgcgcctcc ctccagcagg tgatgccgga ctacatggtg ccctggacct tcgtcgccct
     9721 cgagcgcatg ccgatcaccg ccaacggcaa gctggaccgc aaggcgctgc cggcgccgcg
     9781 ggaggtggcg gaggccggcg cggcgtacga ggcgccgcgc accggcctgg agctcgccgt
     9841 agccggcgtc tggcaggagg tgctgcggct gccgcgggtg gggctgggcg acgacttctt
     9901 cgagctgggg gggagctcgc tcctcgccac gcaggtgatc tcgcgcatcc gccagtcctg
     9961 tggcgtggag ctgccgctgc gcgccctgtt cgaggcgcgc acgctggcgg agatggcgaa
    10021 gcgcgtcgaa tcgttgcagg aaggcccggc attgccggcg acagcggcgc cgccgctgac
    10081 gccggcgccg cgcaccggcc cgctgccgct ctccttcgcg cagcagcggc tctggttcct
    10141 cgaccagctc gtgccgggca gcccgctcta caacatcccc ggcgccctgc ggctgaccgg
    10201 cgcgctggac ccggcgctgc tcggcggcgt cctcgccgag gtggcgcggc gccacgaggt
    10261 gctgcgcacg accttccgcg ccgagggggg cgagccggtg caggtgatcg ccgccgagcc
    10321 ccggctgccg ctgccggtgg tggacctcgc gggcctgccg gccgcgaggc ggcgggagga
    10381 gcgggagcgc cggaccgcgg ccgaggcccg cctcccgttc gacctggcca ccggcccgct
    10441 gctgcgcgcg gcgctgctcc ggctggcccc cggggaccac gtggtgctca tcaccctgca
    10501 ccacatcgtg gcggacgcct ggtccatgga ggtcttctcg cgcgagatcg gggcgctcta
    10561 cggcgcctgg tcccgagagc catcccggag gcggctcccg tcgccgctgc ccgagctggc
    10621 gctgcaatac gccgactacg ccgtctggca gcgccgctgg ctggcgggcg acgccctgga
    10681 ggcggagctg gagtactggc gccggcagct cgccggcgcg ccgcgcgtcc tcgagctgcc
    10741 ggcggaccgg ccgcgcccgg cggtccagag ctaccggggg agctggcggc cgctcgcgct
    10801 gcccgccggc ctgggggcgg agctgaccgg ccgcgcccag cgccggggcg ccaccctctt
    10861 catggccctc ctcggcgcct tcgcgacggt gctcggccgc tacgccgcgc gcgaggacgt
    10921 ggtcgtcggc tcgccggtcg ccggccgcga ccgcccggag atcgaggggc tgatcggctt
    10981 cttcgtcaac accctggtgc tgcgcgtgga cctcgcgggc gaccccggct tcgacggcct
    11041 gctcgaccgg gtccgcgcca tggcgctcga cgccttcgcc caccagagcc tgccgttcga
    11101 gcgcctggtg gacgagctgg cgccggtccg cgatcccagc cactcgccgc tcttccaggt
    11161 ggcgttcgcc ttccagaccg cgccgcgcga gccgctgcgc ctccgcgggc tgacgatgga
    11221 gccgttgcac gacgacagcg gcaccgccaa ggtcgacctg ctgctgtcgc tgaccgaggc
    11281 gggcggggag gtctccggcc tctgggagta cagcaccgac ctcttcgacg aggtcaccat
    11341 cgaccgctgg tcgggccatc tcgcgtccgt gctggcggcg gtggccaccg aggcgggtgg
    11401 cgagggccgc cggctctccg agctgccgct gctcgccccg gccgagcgcc accagctcct
    11461 cagcgagtgg aacgacacgc gcgacgcggt ggacggcgag gcgaccctcg accgtctctt
    11521 cgtcaccacg gccgggcagt ggccggaccg ggtggcggtc agcttcgagg accacagcgt
    11581 cacctaccgg gagctcgccg cccgcgccgg ccgcctggcc cgcacgctgc gggcgatggg
    11641 ggtggagccg gaggtcctgg tggggatctg cgccgaggag tcgatcgagc ggaccgtcgg
    11701 cgtggtcggg accgtgctgg cgggaggagc cttcctgccg ctcgacccgg cgcaccccgc
    11761 ggagcgcctg gagttgatgg ccgaggacgc gcggatccgg gtgctgctga cgcaggagca
    11821 cctgcggccc tcgttgccgg cagccgacgc ggccgtggtc tgcctggacg actacgtccg
    11881 ccaccggcag gagccggtgc accgcgccag ctttccgggc agcctgccgg gaccctgggg
    11941 cccggtgccc gacaacctgt cctacgtcat ctacacctcg ggctcgacgg ggcggccgaa
    12001 cggcgtccag gtctcccacc gctcggcggt gcacctgctc cgccaggcgg cgcgccacac
    12061 cctcgccgac gaggggagcc gcgtgctgca gctcgtctcc ttcagcttcg acgcctcggt
    12121 gctggagacc tggctcgcct tctcgaccgg cggggcgctc tgcgtcatcg gccaggagac
    12181 ccgcctctcg ggtgccgcgc tggccgagac
//
LOCUS       DASPJI010000104         5694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_692221, whole genome shotgun sequence.
ACCESSION   DASPJI010000104 DASPJI010000000
VERSION     DASPJI010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692221"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3114)
                     /locus_tag="VEL74_02735"
     CDS             complement(<1..3114)
                     /locus_tag="VEL74_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HYG61471.1"
                     /translation="MSQQEDAMIPHGIAIVGMAGRFPGARTVTQLWENLRGGVESITF
                     FTDEELLAAGVDPELLRHESYVKAAGALLDTDLFDPSFFGFTPREAEIMDPQHRLLLE
                     CTWEALEDAGYDPERYPGRASVFAGSGAGSYLVFNLLPNPEVMEKVGTLQAILLNDRD
                     FLATRLSYKLNLRGPSVLVQTACSTGLVAVHMASQSLLSGESDLALAGGVSINVPMVD
                     GYLYQEGGISSPDGHCRAFDAQAAGSVSGSGVAMVVLKRLTDAVNDGDHIYAVIRGSA
                     VNNDGSSKVGYTAPSIEGQAEVITEALLMADVPADSLGMVEGHGSGTALGDPIEMSAL
                     IQAFREAGAEGRGFCALGSIKTNVGHLNTAAGTAGLIKTALAVQNGVIPPSLHFEKPN
                     TQVDFVAGPFYVPTEAIPWPEDLTPRRAGVSSFGLGGTNAHVVLEEPPAPSPSGPSRP
                     WQLLPLSARTETALDLATANLAGRLEESLVAGEELDLADVAFTLHVGRRAFSRRRALV
                     CRDREDAVAALRALDSARVISGTYEGSERPVAFLFPGLGDHYPGMARELYGAEPTFAA
                     EVDRCAEALQPHLGVDIRELLFPPGEGEPAGASSGASSGPGRQADLRALLRRGSEGQG
                     SRTLDRTEYAQPVLFVVEYALARLLMEWGIRPQAMIGYSIGEYVAACLAGVLSLEDAL
                     TLVARRAKLIQELPGGGMLAVPLPESDVRGRLPEELSLSATNGPHFTVVGGPEEAVAA
                     FEQRLSGEGVACLRLTTTHAFHSRMMEPAAGALTELARSLSFQEPEVRYLSNVTGTWI
                     TAADLADAGYWGRHMAQPVRFAEGLAELLREPDLILLEVGPGSTLTTLVRQHPGATAD
                     GAARVAISTMRRESERGSDVALLLEAAGRLWAAGAPLGPRGLFAHESRRRVSLPTYPF
                     ERQRCWVDAPRPGERPAGPVLTGGQADAGRAADLADWFYLPGWRRTVPPGRAHGSAVP
                     AEGSWLVFADRSGLGERLAERLRREGNDVVAVTPGPAGSGLAGDEDRYALEPGRREAY
                     CELVTRLR"
     gene            complement(3128..>5694)
                     /locus_tag="VEL74_02740"
     CDS             complement(3128..>5694)
                     /locus_tag="VEL74_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61472.1"
                     /translation="PVAVWMERSLDMLAALLGTLKAGAAYLPIDAGWPAERVETVLAG
                     AGARAILVRRGHLAPVQELQWRLPLLSDLVCLDIAMPEPPPEPLNPGVVRELWDVISD
                     RAVDWVTAGGFVSSYTGQPFAEEEVLEYRDRVIRLADPWLRPDARVLEIGCGSGLILW
                     ELAPRTGRYVGLDPSPRTQERNRERAAAEGVDNVELPTGFAHEIDRFAPGSFDLVILA
                     STAQFFPGPVYLERVLELALSRLAPGGAVVVADLLDPRRCEEFRLSLAAYAAEHPGSA
                     AAAKAVARPGSDGRELYVDEDLFADLEAALPELGRATIHHRRQWLDNELRFRYDVVLE
                     KSAAGGEVTRERRKRLWTGWHVERCPMVRPAASASPIDLAYVIHTSGSTGAPKGIMVQ
                     HRPAVELVGWVNRTFGVGPSDRLLFVTSPAFDLSVYDVFGVLAAGGLVHIASEEMLRD
                     PERLARMLREEPITVWDSAPAALQQLAPLFPEPGSEELRLRLVLLSGDWIPVPLPDLV
                     RASFPRARVISLGGATEATVWSNWYPIGQVDPRWPSIPYGRPIAGARYQVLDAGLEPC
                     PIGVPGDLYIGGRGLCVGYARLPALTAGRFVPDPWSGEVAEPGARLYATGDRARYGRD
                     GNLEFLGRVDQQVKVRGYRIELEEIEAVLTRHPGVREAVVVAREDLPGDPRLVGYVVA
                     SCRPAPAPAELRSALQQTLPEYMVPWTFVEVEALPLTANGKVDRKALPAPQVTAGEGY
                     VAPRNNLERAIATVWREVLGLARVGVHDNFFELGGSSLLVVKLHGRLKQALGNRNLSV
                     MDLFRYSTVDALARYLAAGEARGESSAEQARARTRTRRESLQQLSEARSQRRGRTE"
BASE COUNT          847 a         2116 c         2011 g          720 t
ORIGIN      
        1 gcgcagtcgt gtcaccagct cgcagtaggc ctcccgccgc ccgggctcga gggcatagcg
       61 gtcctcgtcc cccgccagcc cggatccggc cgggcccggg gtcacggcca ccacgtcgtt
      121 gccctcccgg cgcaggcgct cggccagccg ctcgcccagg ccggagcggt cggcgaagac
      181 cagccacgag ccctcggccg gcaccgcgct gccatgggcg cggccgggcg gcacggtgcg
      241 ccgccagccg gggagataga accagtccgc caggtccgcg gcgcggccgg cgtccgcctg
      301 gccccccgtc agcaccgggc cggccgggcg ctcgccgggc ctcggcgcgt cgacccagca
      361 gcgctggcgc tcgaacggat aggtcggcag cgacacccgc cgccggcttt cgtgggcgaa
      421 gagccccctg ggaccgagcg gcgccccggc ggcccagagc cggcccgcgg cctcgagcag
      481 gagcgccacg tccgagccgc gctcgctctc gcgccgcatg gtggagatgg ccacgcgcgc
      541 ggcgccatcc gccgtcgctc ccgggtgctg gcgcaccaga gtggtcagcg tgctgcccgg
      601 ccccacctcc agcaggatca ggtcgggctc gcgaagcagc tcggcgagcc cctcggcgaa
      661 gcgcaccggc tgggccatgt gacgccccca atagccggca tccgccaggt cggcggcggt
      721 gatccaggtg ccggtgacat tggagaggta gcggacctcc ggctcctgga aggagagcga
      781 gcgcgcgagc tccgtgagcg cgccggcggc gggctccatc atgcgcgagt ggaaggcgtg
      841 cgtcgtggtg aggcgcaggc aggcgacccc ctcgccggag agccgctgct cgaaggcggc
      901 gaccgcctcc tcgggaccgc cgacgacggt gaagtgcggc ccgttggtgg cggacagcga
      961 cagctcctcc ggcagcctgc cgcgcacgtc ggactcgggc agcggcacgg cgagcatgcc
     1021 gccgcccggc agctcctgga tcagcttcgc ccgccgcgcc accagggtga gggcgtcctc
     1081 cagcgacagg acgccggcca ggcaggcggc cacgtactcg ccgatgctat agccgatcat
     1141 cgcctgcggc cggatccccc actccatgag caggcgggcc agggcgtact cgacgacgaa
     1201 caggaccggc tgggcgtatt cggtgcggtc gagcgtccgg ctgccctgcc cctcactgcc
     1261 gcggcgcagc agggcgcgca ggtcggcctg ccggcccggc ccggaggacg ctccggaaga
     1321 cgctcccgcc ggctctcctt cgccgggagg aaagaggagc tcgcggatgt ctacccccag
     1381 gtgcggctgc agcgcctcgg cgcaacggtc cacctctgcg gcgaacgtgg gctcggcgcc
     1441 gtacagctcc cgcgccatgc cgggatagtg gtcgcccagg ccggggaaga ggaaggccac
     1501 cggccgctcg ctcccctcgt acgtcccgga gatcacccgc gcggaatcga gggcgcggag
     1561 ggccgccacg gcgtcctcgc ggtcgcggca gaccaacgcg cgccggcggg agaacgccct
     1621 gcgtcctacg tgaagcgtga aggccacgtc ggcgagatcg agctcctccc ccgccaccag
     1681 gctctcctcc agacggccgg ccaggttcgc cgtggcgagg tccagggcgg tctcggtgcg
     1741 ggccgagagc ggcaggagct gccaggggcg cgacgggccg gaaggggagg gcgccggcgg
     1801 ctcctccagc accacatggg cgttggtgcc ccccaggccg aacgagctga cgccggcgcg
     1861 gcgcggcgtc agatcctccg gccacgggat cgcctccgtg gggacgtaga acgggccggc
     1921 gacgaaatcg acctgggtat tgggcttctc gaaatgcagg ctggggggga tcaccccgtt
     1981 ctgcaccgcc agcgccgtct tgatcaggcc ggcggtgccg gcggcggtgt tgagatggcc
     2041 gacgttggtc ttgatcgagc ccagggcgca gaagccccgc ccctcagccc ccgcctcgcg
     2101 gaaggcctgg atgagggcgg acatctcgat gggatcgccc aaggcggtgc cgctgccgtg
     2161 cccctccacc atgccgaggc tgtcagccgg cacgtcggcc atgagcaggg cttcggtgat
     2221 gacctcggcc tgcccctcga tgctcggggc ggtgtagccc accttgctgg aaccgtcgtt
     2281 gttgaccgcc gagccgcgga tcacagcata gatgtgatcg ccgtcgttga cggcgtcggt
     2341 caggcgcttc agcaccacca tcgccacgcc gctaccgctg accgagccgg ccgcctgggc
     2401 gtcgaaggcc cggcagtgac cgtccggcga agagatcccc ccctcctggt agaggtagcc
     2461 atcgaccatc ggcacgttga tcgagacgcc gccggcgagg gcgaggtcgg actctcccga
     2521 cagcaggctc tgggaggcca tgtggaccgc taccaggcct gtggagcagg cggtctggac
     2581 gaggacgctg gggcctcgga ggttcagctt gtacgacagc cgggtggcca ggaagtcgcg
     2641 gtcgttgagg aggatcgcct gcaacgtgcc caccttctcc atcacctcgg gattgggcag
     2701 caggttgaac accaggtagg agccggcccc cgagccggcg aataccgagg cccgcccggg
     2761 gtaacgctcc gggtcgtagc cggcgtcctc caacgcctcc caggtgcact ccaggaggag
     2821 gcgatgctgg gggtccatga tctccgcctc gcgtggcgtg aagccgaaga aggacggatc
     2881 gaacagatcg gtgtcgagga gcgctccggc ggccttgacg tagctctcgt ggcgtagcaa
     2941 ctcgggatct acgccggcgg ccagcagctc ctcgtcggtg aagaaggtga tggactcgac
     3001 gccgccgcgc aggttctccc agagctgcgt caccgtgcgc gctcccggga aacgaccggc
     3061 catgccgacg atggcgatgc cgtgcgggat catggcgtct tcttgctggc tcatcgcgtc
     3121 attcctctca ttcggttcgt ccccgacgct gcgaccgggc ctcactcaat tgctgaagcg
     3181 actcccgtcg cgtccgtgtc cgtgcccgcg cctgctcggc cgagctctcg ccccgcgcct
     3241 cccccgccgc cagatagcgg gccagcgcgt cgaccgtgga gtaccggaag aggtccatga
     3301 ccgacagatt tcggttcccc agcgcctgct tgaggcggcc atggagcttg accaccagca
     3361 acgagctgcc acccagctcg aagaagttgt cgtgcacccc tacccgggcc agccccagca
     3421 cctcccgcca aaccgtggct atggcccgct ccaggttgtt gcgcggcgcc acgtaaccct
     3481 cccccgccgt cacctgggga gccggcagcg ccttgcggtc caccttgccg tttgccgtga
     3541 gagggagcgc ctccacctcc acgaaggtcc acggcaccat gtactccggg agcgtctgct
     3601 gcagcgcgga cctcagctcg gcgggtgcgg gagcgggccg gcaggacgcc acgacgtaac
     3661 ccaccagacg cgggtcgccc ggcaggtctt cccgcgccac caccaccgcc tcgcgcaccc
     3721 cggggtgacg ggtcagcacc gcctcgatct cctcgagctc gatgcggtag ccgcgcacct
     3781 tgacctgctg atcaacccgg cccaggaact ccaggttgcc gtcccgcccg tagcgggcac
     3841 ggtcgccggt ggcgtacagg cgcgcgccgg gctctgccac ctctccagac caggggtccg
     3901 gcacgaagcg cccggcggtc aacgccggca ggcgagcata accgacacag aggccccgcc
     3961 cgccgatata gaggtcgccg gggaccccga tcgggcaggg ctccagcccg gcgtcgagca
     4021 cttgatagcg ggcgccggcg atcggccgac cgtacgggat gctgggccag cgcggatcca
     4081 cctgcccgat gggataccag ttggaccaca ccgtcgcctc ggtggccccg cccaggctga
     4141 tcacgcgggc acgcgggaag gaggcccgca ccagatccgg cagcggcacc gggatccagt
     4201 cgccggacag caggacgagg cgcagccgga gctcctcgct ccccggctcg gggaacaggg
     4261 gggcgagctg ctgcagcgcc gccggggcgg agtcccacac cgtgatgggc tcctcgcgca
     4321 gcatccgcgc caagcgctcc ggatcgcgca gcatctcctc cgaggcgatg tgcaccagcc
     4381 cgccggccgc gagcacgccg aagacgtcgt agaccgacag gtcgaaggcc ggcgaagtga
     4441 cgaacagcag gcggtcggac ggccctacgc cgaaggtccg gttgacccag cccacgagct
     4501 ccaccgccgg ccggtgctgg accatgatcc ccttgggagc accggtggag cccgaagtgt
     4561 ggatgacata ggccaggtcg atgggcgagg cggacgccgc cgggcggacc atcgggcagc
     4621 gctcgacgtg ccagcccgtc cagagccgct tgcgccgctc gcgggtcacc tctcccccgg
     4681 ccgcggactt ctccagcacc acgtcgtacc ggaagcgcag ctcgttgtcg agccactgcc
     4741 ggcggtggtg gatcgtggcc cttcccagct ccggcagggc ggcctccaga tcggcgaaga
     4801 ggtcctcgtc cacatagagc tcccggccat cgctgccggg gcgcgccacc gccttcgccg
     4861 ccgcggcgct gcctggatgc tcggcggcgt acgccgcgag cgacaggcga aactcctcac
     4921 accgccgcgg atcgaggagg tcggccacca ccaccgcccc gcccggcgcc aggcgcgaca
     4981 gcgccagctc cagcacccgc tcaaggtaga ccggcccggg gaagaactgc gccgtgctcg
     5041 ccaggatcac caggtcgaac gaccccggcg cgaagcggtc gatctcgtgc gcaaagccgg
     5101 tgggcagctc gacgttgtcc accccctccg ccgccgcccg ctcgcggttc cgctcctggg
     5161 tgcgcggcga gggatcgagc ccgacgtagc gcccggtccg gggggccagc tcccagagga
     5221 tcaggccgga gccgcagccg atctcgagca cccgcgcgtc cggccgcagc cagggatcgg
     5281 ccagacggat cacgcggtcg cggtattcga ggacctcctc ctccgcgaag ggctgcccgg
     5341 tgtagctgct cacaaagccg ccggcggtca cccagtccac ggcacggtcg gagatgacgt
     5401 cccacagctc ccgcaccacg cccggattca gcggctcggg cggcggctcc ggcatcgcga
     5461 tgtcgaggca gacgaggtcc gacagcagcg gcaggcgcca ttgcagctcc tgcaccggcg
     5521 ccaggtggcc gcggcggacc aggatggcgc gcgctccggc acccgccagc accgtctcga
     5581 cgcgctcggc cggccagccc gcgtcgatcg gcaggtaggc ggctcccgcc ttgagcgttc
     5641 cgaggagggc ggccagcatg tcgagcgagc gctccatcca gaccgccacc ggcg
//
LOCUS       DASPJI010000105         3525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_467573, whole genome shotgun sequence.
ACCESSION   DASPJI010000105 DASPJI010000000
VERSION     DASPJI010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467573"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..923)
                     /locus_tag="VEL74_02745"
     CDS             complement(108..923)
                     /locus_tag="VEL74_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG61473.1"
                     /translation="MTTPRIELRRLTRRLPSGGRELTILDTVDLAITPGEFVAVLGPS
                     GSGKSTLLALMAGLDRPTAGEVLIDGTPIQGMSEDELALVRRRKIGFVFQSFQLLGNL
                     TARENILLPMELQGSPDAASRANELLAAVGLSDRGHHYPTQLSGGEQQRVALARAFAA
                     RPPILLADEPTGNLDGATGRKILEILADLRLQQGTTLVLVTHDPAVAALAGRQIHLRD
                     GRVERDTASEGRGAGAPDRPAQATGSVGGGPGGAHTKEPSTGDPERQRAGALP"
     gene            992..1714
                     /locus_tag="VEL74_02750"
     CDS             992..1714
                     /locus_tag="VEL74_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HYG61474.1"
                     /translation="MRSNVRNLALTLLIPVAWGCGPGASSSPSGEARSAPPPAAPAAT
                     RPAPAGEGRPLVVFLGDSLTAGLGLDEAQAYPALVAQELQEQDRPIRVLNAGVSGDTT
                     AGGLSRLDWLLKQKPDILVVGLGANDALRGQSVAQIEKNLREIVRRSKEAGARVLLLG
                     MQIPPNYGGDYARDYAALYPRIADEMDVALVPFLLEGVGGVPELNLEDGIHPNAEGQE
                     RVAENVAPRLADLLREEKVASE"
     gene            1763..2497
                     /locus_tag="VEL74_02755"
     CDS             1763..2497
                     /locus_tag="VEL74_02755"
                     /inference="COORDINATES: protein motif:HMM:NF017415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HYG61475.1"
                     /translation="MRRELKDTVHRVWLAGLGALATAQQSEREGDTLFRELVKKGEEV
                     EREIDLPYPEIESAGYEVREAAAGLLEDRQLLAAAGVLLERIADQKERMREMIDAMLP
                     PSAVPSPVAVLQARRNAEARAALFAEFGGLTSADVAELAGSKARNKAALANRWKQEGR
                     IFSVPYQGGTYFPGFQFDAQGRPRPVVAEVIAVLGPPVSTEWELALWFMGRHGSVGDR
                     RPVDLLDSEPDAVIQAARYEAEGPVF"
     gene            2547..3143
                     /locus_tag="VEL74_02760"
     CDS             2547..3143
                     /locus_tag="VEL74_02760"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HYG61476.1"
                     /translation="MVWERGKSILRCHDVRFGASEFNPGVGRGRFHPLRDLDGRPVPT
                     LYAADNPEGAFSETIFHEMPVRGPEKILRRLSLVPTVLSTLAATRDLTLAQLHGYGLS
                     RIGVTRSELIEVGSEHYGETAAWARALHACDGRDGRIDGLVWVSRQHDDAYALVLFGD
                     RVARRDLVVVEPPLPLYQGPGYLAVQSAAERAGITIIE"
     gene            complement(3133..>3525)
                     /locus_tag="VEL74_02765"
     CDS             complement(3133..>3525)
                     /locus_tag="VEL74_02765"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HYG61477.1"
                     /translation="VPGSLPALLGAYRLTQKAAGVGFDWPDAASVLAKAEEETAELRQ
                     ALTDEGPGQREAVKEEVGDLLFTLANLARKLEVDPEAALAGTNRKFRQRFEHVEKGLE
                     TRGKTAAEATLEEMDALWEEAKGERPTR"
BASE COUNT          493 a         1190 c         1322 g          520 t
ORIGIN      
        1 ggcgaccacg gcggccacgc ccacggccag gcaggcgacg aagaaggtga ggcggccgcg
       61 tgagccccgg gattcgcgga ggagggtctt gaaatagaag gagagactca cgggagggct
      121 cccgcccgct ggcgctccgg atcgcccgtg ctcggctcct tcgtgtgcgc tccgcccggc
      181 ccccctccca cgctgccagt cgcctgcgcc gggcgatccg gagcgccagc cccccgcccc
      241 tcggatgcgg tatcccgttc gacccggccg tcccggagat ggatctggcg gccggcgagg
      301 gcggcgacgg cggggtcgtg ggtgacgagg acgagggtgg tgccctgctg gagacggagg
      361 tcggcgagga tctccaggat cttgcggccg gtcgcgccgt cgaggttgcc ggtgggctcg
      421 tcggccagga ggatcggcgg gcgggcggcg aaggcgcggg ccagggcgac gcgctgctgc
      481 tcgccgccgg agagctgagt ggggtagtgg tggccgcggt cggagaggcc gacggcggcc
      541 agcagctcgt tcgcccgcga ggcggcgtcg ggggagccct gcagctccat ggggaggagg
      601 atgttctccc gcgccgtgag gttgcccagg agctggaagc tctggaagac gaagccgatc
      661 ttcctgcggc gcaccagggc cagctcgtcc tcggacatcc cctggatcgg cgtgccgtcg
      721 atcagtacct cgcccgcggt ggggcggtcg aggccggcca tcagggcgag gagcgtcgac
      781 ttgccgctcc ccgagggccc cagcacggcc acgaactcgc ccggcgtgat cgccaggtcg
      841 acggtatcca gaatggtcag ctcgcgcccc ccggagggaa ggcggcgggt gagacgtctc
      901 agctcgatgc gcggcgtcgt catcggatct ccacggggtc cacgagccgg gctgggaaca
      961 aataggctcg gtctcggaat tgatagggac catgaggtcc aacgtccgaa atctcgccct
     1021 gactttgctt attcccgtcg cctgggggtg tggccccggc gcgtccagca gcccctccgg
     1081 ggaggcccgg tccgcgcctc cgcccgccgc ccccgcggcc acccggcccg cccctgccgg
     1141 cgagggccgg cctctggtgg tcttcctggg cgacagcctc accgccggat tggggctcga
     1201 cgaggcccag gcctatccgg ccctggtggc tcaggagctc caggagcagg accggcccat
     1261 ccgcgtgctc aacgccgggg tgagcggcga caccaccgcc ggcggcctgt cgcgcctgga
     1321 ctggttgctg aaacagaagc ccgacatcct ggtggtgggg ctcggcgcca acgacgccct
     1381 tcgcgggcag tccgtagcgc agatcgagaa gaacctgcgg gagatcgtcc gccgctcgaa
     1441 ggaggccggc gcccgggtcc tcctcctcgg catgcagatc cccccgaact acggcggcga
     1501 ctatgcccgc gactacgccg ccctctatcc gcggatcgcc gacgagatgg acgtggcgct
     1561 cgtccccttc cttttagaag gggtgggcgg cgtcccggag ctgaacctgg aggacggcat
     1621 ccaccccaac gccgagggac aggagagggt ggcggagaac gtggccccgc gcctcgcgga
     1681 cctgctgcgg gaggagaagg tcgcctcaga gtgatggaag cgcgcgttag gacgtggtat
     1741 catttcgatc atggaggcca ccatgcggcg cgagctgaaa gacacggtcc acagagtctg
     1801 gctggcgggg ttgggagcct tggccacggc ccaacagtcc gagcgggaag gtgataccct
     1861 cttccgggaa ctggtgaaaa agggagaaga ggtggagcgg gagatcgatc tgccgtaccc
     1921 ggagatcgaa agcgcgggct acgaggtgcg cgaggcggcg gccggcctgc tggaggaccg
     1981 gcagctcctg gcggcggccg gggtgctgct ggagcggatc gccgatcaga aagagaggat
     2041 gcgggagatg atcgacgcca tgttgccccc gtcggcggtg ccgagccccg tggccgtgct
     2101 ccaggcgcgg cgcaacgcgg aggcgcgggc ggccctgttc gcggagttcg gcgggctgac
     2161 cagcgccgat gtggcggagc tcgccgggtc gaaggcgcgc aacaaggcgg cgctggcgaa
     2221 ccgctggaag caggaggggc gcatcttctc cgtcccctac caggggggca cctactttcc
     2281 cggcttccag ttcgacgccc aggggcggcc gcggccggtc gtcgcggagg tgatcgccgt
     2341 cctcggaccg ccggtgagca ccgaatggga gctggcgctc tggttcatgg gccgacatgg
     2401 ctcagtaggg gaccgccggc cggtcgacct cctggacagc gagccggatg ccgtcatcca
     2461 ggccgcgcga tacgaggccg aggggcctgt cttctaaggg cccgctcccg gggcctcccg
     2521 atccggtctc gctcgctccg ctcctggtgg tctgggagcg ggggaagtcc atcctgcgct
     2581 gccacgatgt ccgcttcggc gccagcgagt tcaatccggg tgtcgggcgg ggccgcttcc
     2641 atcccctgcg tgacctcgac gggcggcctg tgccgacgct ctacgccgcc gacaacccgg
     2701 aaggcgcctt ctccgagacc atctttcatg aaatgccggt acgggggccg gagaagatcc
     2761 tccgccgcct gtcgctggtg cccaccgtgc tctcgaccct ggcggcgacg cgggatctga
     2821 cgctggcgca gctccatgga tatggtttgt cgaggatcgg cgtcacccgc tccgagctga
     2881 tcgaggtggg cagcgagcat tatggcgaga ccgccgcctg ggcacgggcg ctccacgcct
     2941 gcgacggccg cgacggccgc atcgatgggc tggtctgggt gtcgcggcag cacgacgatg
     3001 cctatgcgct cgtcctcttc ggggaccggg tggcgcggcg ggacctggtg gtggtggagc
     3061 cccctctccc cctctatcag ggaccggggt acctggcggt gcagagcgcc gccgaacggg
     3121 cggggatcac catcatcgag taggccgctc ccctttggcc tcctcccaga gggcgtccat
     3181 ctcctccagg gtggcctcgg cggccgtctt gccgcgggtc tcgaggccct tctcgacgtg
     3241 ctcgaagcgc tgccggaact tgcggttggt gccggccagg gcggcctcgg ggtcgacctc
     3301 cagcttgcgc gccaggttgg ccagggtgaa caggaggtcc cccacctcct ccttgaccgc
     3361 ctcgcgctgc ccggggcctt cgtccgtcag ggcctgccgc agctcggcgg tctcctcctc
     3421 ggccttggcg aggaccgagg cggcgtccgg ccagtcgaag ccgacgccgg ccgccttctg
     3481 agtcagccgg taggcgccga ggagcgcggg gagtgatccg gggac
//
LOCUS       DASPJI010000106        12905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_349273, whole genome shotgun sequence.
ACCESSION   DASPJI010000106 DASPJI010000000
VERSION     DASPJI010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12905)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12905
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349273"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="VEL74_02770"
     CDS             complement(<1..1130)
                     /locus_tag="VEL74_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYG61478.1"
                     /translation="MAEILQSPSRQVLPSACPLDCPDACSLEVQVEDGRVVKVDGTHL
                     NPITQGYICAKVRRLPEHLYGPDRLLYPARRVGRKGEASFERISWDEALDLVAERMRQ
                     ARERWGGESVLPLSYGGSNGLLTQDTTDARLFYRFGASRLARTVCAAPTNRAAAGLYG
                     KMTGVVPQDFVHSRLIVLWGVNPSATGIHHVPILQEAQRRGARLVVIDPRRTPLAKKA
                     DLHLAPFPGADLPVALSVLRWLFEEGRADLEFLAAHATGWEELRRRAAPWTFGRAAEA
                     SGVPAADLELFARLYAGTSPAVVRAGWGFERNRNGGSAVAAALALPAVAGKFGVRGGG
                     YLMSNSGAWSFDRAGAMGAAEPATRLINMNRLGETLAERDDPP"
     gene            complement(1170..2198)
                     /locus_tag="VEL74_02775"
     CDS             complement(1170..2198)
                     /locus_tag="VEL74_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HYG61479.1"
                     /translation="MGKTKITLLPGDGIGPEVTNATVRVLDAAGADLEYERHVAGAEA
                     VQQFGNPLPPEVMESIQRNCIALKGPITTQVGGGFQSVNVQLRQKLELYANLRPSRSL
                     PGIPNRFENVDIVVVRENTEDLYSGLEHTVVPGVVESIKIITEKASLRIARFAFEYAR
                     RHGRKRVTAVHKANIMKLSDGLFLDCFRRVALDYPDIQADDRIVDNMCMQLVMRPEEW
                     DVLLLENLYGDIVSDLCAGLVGGLGVVPGANIGLESAVFEAVHGSAPDIAGKDLANPL
                     ALIRSAILMLWHLHKDEAAHRIRAALRHVVVRQKILTRDLGGDASTTQFTDAVVAAIE
                     SGAGEDLM"
     gene            complement(2313..5171)
                     /locus_tag="VEL74_02780"
     CDS             complement(2313..5171)
                     /locus_tag="VEL74_02780"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG61480.1"
                     /translation="MALQREQVVQTAEKYVSRGKIEPAIREYKKLLAENPNDINTLNR
                     VGDLYAKIHRVEEAIDFFTQIAERYTAEGFFVKAIAIYKKIIKLDPTRLEVYEKLAEL
                     YHKQGLVNEARTQYQVLADYYQKHDNAASAIAIYLKMTELEPENPSYHVKLAEIYQQQ
                     QLIEKAMGEYRVIAELMINHGHAQEAAQVYERALDVDSRSVGFIRDAVRRLEAAGHHA
                     GAARLLSLAVERNPAAREVHLADAGAEAEPQPQSQMMEADSSPELVAEDFQPAPEEDI
                     YDSMSMGGPSLMERLAELTPTEDDDAFGRLDDDLASAMRAAASAVPGSAQEPARPAAG
                     GADSGDEIELDLDEVFVLDFAEDDEEPASLVKPPADMLDDQPRRPAWAQASGTISETP
                     VPAAPPPAAPEPSFEPAFEAAPAPSWQAAEEAAEEVEIELDLDFDNLSFGETPAAPAA
                     QPSAEAVDEAPAAEEALVLEELHLEPMDFSVGLEDFELSEPAPAPAAAAPPAAQPMEL
                     DHDLLERTAAELDPRQLDRDEDLVAEAEVLAKYGLEEKALERLREALRLRPRHLDAYS
                     LMIQIHLDKGRHARVMEVANQMASIAAQVGDGTAWPKVRKRLTEAGYRVEGDHVAAAP
                     RGRKDGDEAVQDLLKGLLDKPARKAPKPAAAAPPPPAEPPPDVAPEPPPPLEAAAAFS
                     PPPQPAPPRVERPGIFNPLEIGELVEAEDMDEPTPFAPGPSVTPAGGDAPPPWLVAMS
                     GASVAPSAPGSALDDSGMSWLDESNGRSQPQESGGLFDEDDDFFDLAGELEQELSQED
                     IFQDDTSLMPAEQSLEEIVEGFKKGVAEHLSPTDYDTHFNLGIAYREMGLLDEAIGEF
                     QLASKSPGHLVSCCSMLGLCFLDKGLPELAIKWYQRGLAAPNVSEEDSLGLLYDLGNV
                     YLSIGDNPTAYRTYVELYGINSNYRDVVAKLEELGRSR"
     gene            complement(5171..5788)
                     /locus_tag="VEL74_02785"
     CDS             complement(5171..5788)
                     /locus_tag="VEL74_02785"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HYG61481.1"
                     /translation="MRLLRGLEELLAQCGYGLIAGVDEVGRGCLAGPVVAAAVIVDPR
                     CLVPGVDDSKCLTAAERERVAAAIRATAVATAVAPVPPDVIDRVNILEATRLAMRKAL
                     SSLRPAPDCAVVDAVRLSGFRFPCLPVVRGDSISYAVACASILAKVERDRMMVDLDRR
                     YPHYGFAAHKGYSVPEHMAALAAYGPCPEHRLTFRQVVPRAEELC"
     gene            complement(5900..6265)
                     /gene="rplS"
                     /locus_tag="VEL74_02790"
     CDS             complement(5900..6265)
                     /gene="rplS"
                     /locus_tag="VEL74_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HYG61482.1"
                     /translation="MNELQQVEQPYVQREVPEFRPGDTVRVHVRVVEGDKERIQVFQG
                     VIIARRGGGTRETFTVRKVSGGIGVERIFPLHSPNVDRIEVIRRGRVRRAKLYYLREL
                     RGKAARIEERRDERPAAGR"
     gene            complement(6367..7140)
                     /gene="trmD"
                     /locus_tag="VEL74_02795"
     CDS             complement(6367..7140)
                     /gene="trmD"
                     /locus_tag="VEL74_02795"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HYG61483.1"
                     /translation="MRVQVLTIFPELFGPFLATSLVGRAIEKGLLEARVHDLRDFTED
                     RHRSVDDEPYGGGGGMVMMAPPWIRAVRAVAGQGGEGSWRVLMSPQGRRLDEAKVRQL
                     AARGDLVLLCGRYEGIDERVLSTVVDEEVSIGDYVLSGGELPAMVLIEAVSRQIPGVV
                     GLAESVERDSFRQGLLDHPHYTRPPVVEGLAVPEVLLSGHHAEIQRWRDREALKATLA
                     KRPDLVERLLERPEALESLTREQRRELMEWNAGRSCAGS"
     gene            complement(7122..7724)
                     /gene="rimM"
                     /locus_tag="VEL74_02800"
     CDS             complement(7122..7724)
                     /gene="rimM"
                     /locus_tag="VEL74_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949997.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HYG61484.1"
                     /translation="MSSRSSRTSEVRSDLPETVAVGRILRPHGVRGEVVVEVLSDVPG
                     RFDPGSSLLTSREREDRQRDGRTAGSLTSPRLIVAASRPHKSGALVVFEGVDDRDRAE
                     ELRGVWLEVPRAEVPAAEPGTYYEYELLGCRCFVGGEELGRVEELREDGGGLLLIVDD
                     GERRIPVPFVRAFLRDVDVAGGRIELDLPEGLLEACASRS"
     gene            complement(7696..7941)
                     /locus_tag="VEL74_02805"
     CDS             complement(7696..7941)
                     /locus_tag="VEL74_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HYG61485.1"
                     /translation="MPELKDDFEELARLLVDQPEEVEVTEIPGRRDSVLELKVAPDDL
                     GKVIGRQGRTARALRTLLDTRADRLGERYELEILEDE"
     gene            complement(7934..8176)
                     /gene="rpsP"
                     /locus_tag="VEL74_02810"
     CDS             complement(7934..8176)
                     /gene="rpsP"
                     /locus_tag="VEL74_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HYG61486.1"
                     /translation="MLKIRLRRMGARNNPFYRVVVSDSRNTPTASAVEEIGHYDPTKN
                     PAQVTIDAERAQYWVSRGAHMSPTVSKLLAQAKKSA"
     gene            complement(8298..9635)
                     /gene="ffh"
                     /locus_tag="VEL74_02815"
     CDS             complement(8298..9635)
                     /gene="ffh"
                     /locus_tag="VEL74_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HYG61487.1"
                     /translation="MFDGLQTKLQDVFRNLKGEGKVTEESLQAALRQIRLALLEADVH
                     LRVVKPFIERVRERALGQEVLQSLTPAQQVVKIVRDELTSLLGEEGTELRLDGRPAVI
                     MLCGLQGSGKTTTAGKIAKRLKKRGKHPLLVAGDLQRAAAVEQLKQVGKGVEVPVVEP
                     EPGESVIDVGARALNVARQRGHDVVIMDTAGRLHVDAALMEEIKRLADRIQPHEVLFV
                     ADSMTGQDAVKSAEQFGQTLPLTGVVLTKLDGDSRGGAALSIRTVTRVPIRFVGMGEK
                     SDDLELFHPERLSSRMIGMGDVLSLIERAEQGIDQEEAKRLAGRIARQEFTLEDLRDQ
                     LRQIRRLGPLGQVLELLPKVGPLRGLEGADVDESGLSRVEAIINSMTPLERQRPDVIN
                     GSRKKRIAMGSGTSVQEINRLLKQHKSMKKMLKGVQGKWLRKALGGGAAMPPQ"
     gene            complement(9970..10950)
                     /locus_tag="VEL74_02820"
     CDS             complement(9970..10950)
                     /locus_tag="VEL74_02820"
                     /inference="COORDINATES: protein motif:HMM:NF016629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HYG61488.1"
                     /translation="MGRHDSGYKQLFAHARVVADLLRRFVREPWVRQLDFATLARVSG
                     DFMSGDLTSRHNDVVWRLLWKRPGAAPTPVYLLLEFQATPERFMAVRLLTYLSLLYED
                     IIRNRALTPSGKLPPVLVMVIYNGNRRWNAARDLESLIEPAPGTLGRSCPRFTYTVLD
                     ERRIAPGELGDASNLAVALIRLEVSHSLEEILAVAAELSAQLPRDREPELRQAFTGWL
                     LRALRRAHPGAVIPDRADLEEIPMLEENLIRWAREDRRRARQEGHKAGERKGRVEGAR
                     NLLLRQLERRFGPLAPEVRQRVGAISSVRKLEAMADQILVVGSLRDLGLE"
     gene            11090..12571
                     /gene="pyk"
                     /locus_tag="VEL74_02825"
     CDS             11090..12571
                     /gene="pyk"
                     /locus_tag="VEL74_02825"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HYG61489.1"
                     /translation="MERRAKIMATLGPASTQEDVLQELIHAGVDLIRLNLSHGTQDDH
                     RKTLHLVRRVAEQEGCHLPVVMDLMGPRYRLGKIPGERRMLEPGEELVLGTQSEGVDL
                     PVDSEILSHLEAGERVLIDSGLIELKVESKEGDRVSARVVNGGPVRTRKGINLPDTSL
                     PFEISEKDRSDIAFAVAEGADYLAASYVGEASHIEALREVVTQAGGRIPIVAKLERAT
                     AMEHLDEITEAADGLMVARGDLGVEVPLHTVPVHQKRIVAAGRRAGKPVIVATQMLES
                     MIEQPRPTRAEATDVANAVLDGADALMLSGETAMGKYPVGAVSTMARIILEAEEYARE
                     CATDERPSIHPLGPVHAQRTFALDPSASDEGSESTLDVPDLVSAAAVYAADQLEVRRI
                     VAFSQSGFTARLVARYRPDAPVVAFTPDERVARQLQLVWGVRPLLADIEVGTLDEVVQ
                     LVQRQLLAARLVEPGERIIILMGHPIRDRPLTNLMRVHRIRAV"
     gene            12603..>12905
                     /locus_tag="VEL74_02830"
     CDS             12603..>12905
                     /locus_tag="VEL74_02830"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYG61490.1"
                     /translation="MLPLHDINPRRSFPAVTVVLIAVNVLLFFYELSLGPHLEAFMLS
                     SAFVPARLFDDGAIYASEIGPEARSALLSMFLHGGWGHLLGNMLFLWVFGDNIEDRL"
BASE COUNT         1797 a         4582 c         4568 g         1958 t
ORIGIN      
        1 ggcgggtcgt cccgttcggc cagcgtctcg ccgaggcggt tcatgttgat cagccgggtg
       61 gccggctcgg ccgcgcccat ggcgccggcg cggtcgaagc tccaggcgcc cgagttgctc
      121 atcaggtagc cgccgccgcg caccccgaac ttgccggcca cggcggggag cgccagggcc
      181 gcggccaccg ccgacccgcc gttgcggttg cgctcgaagc cccagccggc gcgcacgacc
      241 gccggcgagg tgccggcgta gaggcgcgcg aagagctcca ggtcggccgc cggcactccc
      301 gacgcctccg cggcgcgccc gaaggtccag ggggcggcgc gccggcgcag ctcctcccag
      361 ccggtggcgt gggcggcgag gaactcgagg tccgcccgcc cctcctcgaa cagccagcgc
      421 aggaccgaga gcgccaccgg caggtcggcg cccggaaagg gggcgagatg caggtcggcc
      481 ttcttcgcca gcggcgtgcg gcgcgggtcg atcaccacca gccgcgcgcc gcggcgctgc
      541 gcctcctgga ggatgggcac gtggtggatg cccgtggccg aggggttcac cccccagagg
      601 acgatcagcc gcgagtggac gaagtcctgc ggcaccaccc cggtcatctt gccgtagagc
      661 ccggcggcgg cgcggttcgt gggggcggcg cagacggtgc gggcgaggcg cgaggcgccg
      721 aaccggtaga agagccgggc gtcggtggtg tcctgggtga gcaggccgtt cgatccgccg
      781 taggagagcg gcagcaccga ctctcccccc cagcgctcgc gcgcctggcg catccgctcc
      841 gcgaccaggt cgagcgcctc gtcccaggag atccgctcga acgaggcctc ccccttccgg
      901 cccacgcggc gcgccgggta gagcagccgg tccgggccgt agaggtgctc gggcaggcgg
      961 cgcaccttgg cgcagatgta gccctgggtg atcgggttga ggtgcgtgcc gtcgaccttg
     1021 accacgcggc cgtcctccac ctgcacctcc aggctgcagg cgtccggaca gtcgaggggg
     1081 caggcgctgg ggagcacctg gcgggaggga gactggagga tctcggccat gaggggattc
     1141 tatccccccg cggcctcctg gcggcgcctc tacatcaggt cttcgcccgc gccggactcg
     1201 atggccgcca ccacggcgtc ggtgaactgg gtggtcgagg cgtcgccgcc gaggtcgcgg
     1261 gtgaggatct tctggcgcac caccacgtgg cgcagggcgg cgcggatgcg gtgggccgcc
     1321 tcgtctttgt gcaggtgcca gagcatcagg atcgccgagc ggatcagggc gaggggattg
     1381 gcgaggtcct tgccggcgat gtccggcgcg ctgccgtgga ccgcctcgaa caccgcggac
     1441 tcgaggccga tgttggcgcc gggcaccacg ccgaggccgc cgaccaggcc ggcgcagagg
     1501 tccgagacga tgtcgccgta gaggttctcg agcagcagca cgtcccactc ctcgggccgc
     1561 atgacgagtt gcatgcacat gttgtcgaca atcctgtcgt cggcctggat gtccgggtag
     1621 tcgagcgcca cgcggcggaa gcagtcgagg aagaggccgt ccgagagctt catgatgttg
     1681 gccttgtgga cggcggtcac ccgcttgcgg ccgtggcggc gggcgtactc gaaggcgaag
     1741 cgggcgatgc gcagcgacgc cttctcggtg atgatcttga tgctctccac caccccgggg
     1801 acgacggtgt gctcgaggcc gctgtagagg tcctcggtgt tctcccgcac caccacgatg
     1861 tcgacgttct cgaagcggtt ggggatgccc ggcagcgacc gcgaggggcg caggttggcg
     1921 tagagctcga gcttctggcg gagctggacg ttgaccgact ggaagccgcc gccgacctgg
     1981 gtggtgatcg gccccttgag cgcgatgcag ttgcgctgga tggactccat cacctccggc
     2041 gggagggggt tgccgaactg ctggacggcc tcggcgccgg cgacgtggcg ctcgtattcg
     2101 aggtcggcgc cggcggcgtc gagcacccgt acggtggcgt tggtgacctc cggcccgatg
     2161 ccgtcgccgg ggaggagagt gatcttggtc ttgcccatgg aatgcgctcc gtcgtcgtcg
     2221 agcccgccgg tttcctggag aaggtgccgc ggtgcggctc ccggccgggg gcggctaggg
     2281 tatcacgagg ggcgaggcgg tccggaaggg gctcagcggg agcgccccag ctcttcgagc
     2341 ttcgccacca cgtcgcggta gttgctgttg atgccgtaga gctccacgta ggtgcggtag
     2401 gccgtggggt tgtcgccgat cgagaggtag acgttgccca ggtcgtagag caggcccagg
     2461 ctgtcctcct cggagacgtt gggggcggcg aggccgcgct ggtaccactt gatggccagc
     2521 tcggggagac ccttgtccag gaagcagagg ccgagcatcg agcagcagga gacgaggtgg
     2581 cccgggctct tcgaggcgag ctggaactcg ccgatcgcct cgtcgagcag ccccatctcg
     2641 cggtaggcga tgccgaggtt gaagtgggtg tcgtagtcgg tcggggacag gtgctccgcg
     2701 acccccttct tgaacccctc gacgatctcc tccagcgact gctccgccgg catcagggag
     2761 gtgtcatcct ggaagatgtc ctcctgggag agctcctgct ccagctcgcc ggccaggtcg
     2821 aagaagtcgt cgtcctcgtc gaagaggccg ccgctctcct gcggctggct gcggccgttg
     2881 ctctcgtcga gccagctcat gccggagtcg tcgagggcgg agccgggcgc ggacggcgcc
     2941 acggaggccc ccgacatcgc caccagccag gggggcggcg cgtcgccgcc ggccggggtc
     3001 acactcggac ccggagcgaa cggcgtgggc tcgtccatgt cctccgcctc gaccagctcg
     3061 ccgatctcca gcgggttgaa gatccccggc cgctccaccc gcggcggcgc cggctggggc
     3121 ggcgggctga acgccgccgc ggcctccagg ggaggcggcg gctccggggc gacgtccggg
     3181 ggcggctcgg caggaggagg aggcgcggcc gcggcgggct tcggcgcctt gcgggccggc
     3241 ttgtcgagca gccccttgag gaggtcctgg acggcctcgt cgccgtcctt gcggccgcgc
     3301 ggcgcggcgg ccacgtggtc gccttccacc cggtagccgg cctcggtcag gcgcttgcgg
     3361 accttgggcc aggccgtgcc gtcgcccacc tgcgccgcga tcgaggccat ctgattggcc
     3421 acttccatga cgcgggcgtg gcgccccttg tcgagatgga tctggatcat cagggagtag
     3481 gcgtcgagat gccgcggccg cagccgcagc gcctcgcgca ggcgctccag ggccttctcc
     3541 tccaggccgt acttggcgag cacctcggcc tcggccacca ggtcctcgtc ccggtcgagc
     3601 tgccgcgggt cgagctccgc cgcggtgcgc tcgagcaggt cgtgatcgag ctccatgggc
     3661 tgcgcggcgg gaggggcggc ggcggccggc gcgggtgcgg gctccgatag ctcgaagtcc
     3721 tccagcccga cgctgaagtc catcggctcg aggtgcagct cctcgaggac gagggcctcc
     3781 tcggcggccg gcgcctcgtc cacggcctcc gcggacggct gtgccgccgg cgccgccggg
     3841 gtctcgccga aggagaggtt gtcgaagtcc aggtcgagct cgatctccac ctcctcggcg
     3901 gcctcctcgg ccgcctgcca ggaaggggcg ggcgccgcct cgaaagcagg ctcgaaggag
     3961 ggctccggag cggcgggcgg cggggccgcg gggaccggcg tctcggatat cgtccccgag
     4021 gcctgggccc aggcggggcg ccgcggctgg tcgtcgagca tgtccgccgg cggcttgacc
     4081 aggctggcgg gctcttcgtc gtcctcggcg aagtcgagga cgaacacctc gtccaggtcc
     4141 agctcgatct cgtcgccgct atccgcgccg ccggccgcgg gccgcgcggg ctcctgggcg
     4201 gagccgggga cggcggaggc cgccgcccgc atggcgctgg cgaggtcgtc gtcgagccgg
     4261 ccgaaggcgt cgtcgtcttc cgtcggcgtc agctcggcga ggcgctccat gagcgagggc
     4321 ccgcccatgg acatcgagtc gtagatatct tcctcgggcg ccggctggaa gtcctcggcc
     4381 accagctcgg gggagctgtc cgcctccatc atctgggatt ggggctgggg ctccgcctcc
     4441 gcgcccgcgt ccgcgagatg gacctcgcgg gcggccgggt tgcgctcgac ggccagggag
     4501 agcaggcggg ccgcgccggc gtggtgcccc gcggcctcca gccggcgcac ggcgtcgcgg
     4561 atgaagccga cgctgcggct gtcgacgtcc agggcgcgct cgtagacctg cgccgcctcc
     4621 tgggcgtggc cgtggttgat catcagctcg gcgatgacgc ggtactcccc catcgccttc
     4681 tcgatgagct gctgctgctg atagatctcg gccagcttga cgtggtagct cgggttctcc
     4741 ggctcgagct ccgtcatctt caggtagatg gcgatggcgg aggcggcgtt gtcgtgcttc
     4801 tggtagtagt cggccagcac ctggtactgg gtgcgcgcct cgttgaccag cccctgcttg
     4861 tggtagagct ccgccagctt ctcgtagacc tcgagccgcg tcgggtcgag cttgatgatc
     4921 ttcttgtaga tggcgatggc tttgacgaag aagccctcgg ccgtgtagcg ctcggcgatc
     4981 tgggtgaaga agtcgatcgc ctcctcgacg cggtggatct tggcgtagag gtccccgacg
     5041 cggttcagcg tgttgatgtc gttcgggttc tcggcgagca gcttcttgta ctcgcggatc
     5101 gccggctcga tcttgccgcg ggagacgtac ttctcggcgg tctggacgac ctgctcgcgc
     5161 tgcagggcca tcagcaaagc tcctcggcac gaggtacgac ctgccggaag gtcaggcggt
     5221 gctcggggca ggggccgtag gcggcgagcg ccgccatgtg ctcgggcacc gaatacccct
     5281 tgtgggcggc gaagccgtag tgcggatagc ggcggtcgag gtccaccatc atgcggtcgc
     5341 gctccacctt ggcgaggatg gaggcgcagg ccacggcata gctgatggag tcgccgcgca
     5401 ccaccggcag gcaggggaag cggaagccgg agaggcggac ggcgtcgacc acggcgcagt
     5461 ccggcgccgg gcgcagggac gacagggcct tgcgcatggc gagccgggtc gcttccagga
     5521 tgttgacgcg gtcgatgacg tcgggcggga cgggggcgac ggcggtggcc acggcggtgg
     5581 cgcggatcgc cgcggcgaca cgctcgcgct ccgccgcggt caggcacttg ctgtcgtcga
     5641 cccccggcac caggcagcgg ggatcgacga tcacggcggc ggccacgacg gggccggcga
     5701 ggcagccgcg gcccacctcg tcgacgccgg cgatcaggcc gtagccgcac tgggccagca
     5761 gctcttccaa gccacgcagc agccgcaggc ggtacgcctc ggcaaacaga tccgtgagcc
     5821 cccgggcctg gggggttgcc gccctggtca ccgcggctcc tttcctgacg actaccgcac
     5881 ctgccgccgg cgttcgaagc taccggcccg cggccggacg ctcgtcgcgg cgctcttcga
     5941 tgcgcgccgc cttgccccgc agctcgcgga ggtagtagag cttggcgcgg cgtacccggc
     6001 ctcgccgaat gacctcgatc cggtcgacgt tgggcgaatg cagggggaag atgcgctcca
     6061 ccccgatgcc gcccgacacc ttgcggacag tgaaggtctc ccgggtgccg ccgccgcgac
     6121 gggcgatgat caccccctga aagacctgga tcctctcctt gtcaccctcg accacgcgga
     6181 cgtggacgcg gaccgtgtcc cccggacgga actccgggac ctcgcgttgc acatacggtt
     6241 gttcgacctg ctgcagctcg ttcatggctt cacctcgtca aaacccgacg gacgcctagg
     6301 ggacggtccc gtcattattc ctgtttcaga atggccgcgc aagccggcgc gtctccgctc
     6361 cggcattcag ctccccgcgc agctccttcc ggcattccat tccatcagct cccggcgctg
     6421 ctcccgggtc aacgactcca gggcctcggg gcgctccagc aagcgttcca ccagatcggg
     6481 gcgcttggcc agcgtcgcct tcaacgcctc gcggtcccgc cagcgctgga tctccgcgtg
     6541 gtgccccgac agcagcacct ccggcaccgc gagcccctcg accaccggcg gccgcgtgta
     6601 gtgcgggtgg tccaggagcc cctgccggaa gctgtcgcgc tccaccgact cggcgagccc
     6661 gacgacgccg gggatctggc gcgacaccgc ctcgatcagc accatggcgg gcagctcgcc
     6721 gcccgacagc acgtagtcgc cgatcgagac ctcctcgtcc accaccgtcg agagcacccg
     6781 ctcgtcgatc ccttcgtagc ggccgcagag cagcaccagg tcgccccgcg ccgccagctg
     6841 ccgcaccttg gcctcgtcga gccgccgccc ctggggggac atcagcaccc gccaggagcc
     6901 ctcgcccccc tggccagcca ccgcccgcac cgccctgatc cagggcggcg ccatcatgac
     6961 catcccgccg ccgccgccgt acggctcgtc gtcgacgctg cggtggcggt cctccgtgaa
     7021 gtcccgcagg tcgtgcaccc gggcctccag cagccccttc tcgatcgccc tccccaccag
     7081 gctggtggcg aggaagggcc cgaacagctc ggggaagatc gtcaggacct ggacgcgcat
     7141 gcctcgagca gcccttccgg caggtccagc tcgatccgcc cgcccgcgac gtcgacgtcg
     7201 cggaggaacg cgcgcacgaa cggcaccggg atgcggcgct cgccgtcgtc gacgatcagg
     7261 aggaggccgc cgccgtcctc ccgcagctcc tccacacgcc ccagctcctc gccgccgacg
     7321 aagcagcggc agcccaggag ctcgtactcg taataggtcc cgggctcggc ggcgggcacc
     7381 tcggcgcgcg gcacctcgag ccagacgccg cgcagctcct cggcccggtc gcggtcgtcg
     7441 accccctcga agaccaccag cgcccccgac ttgtgggggc ggctcgcggc cacgatgaga
     7501 cgggggctcg tcaggctgcc ggccgtccgg ccgtcgcgct gcctgtcttc gcgttcgcgc
     7561 gacgtcagca gcgagctgcc cgggtcgaag cggccgggga cgtccgagag cacctccacc
     7621 accacctcgc cgcgtacgcc gtggggccgc aggatgcggc cgaccgcgac ggtctcgggc
     7681 aggtcgctcc ggacctcact cgtcctcgag gatctcgagc tcatagcgct cgcccagccg
     7741 gtccgcccgg gtgtccagca gagtgcgcag cgcccgggcg gtccgcccct ggcgtccgat
     7801 caccttgccc aggtcgtcgg gggccacctt gagctccagc acgctgtcgc ggcgcccggg
     7861 gatctcggtc acttcgacct cctcgggctg atcgaccagc aggcgggcca gctcttcgaa
     7921 gtcgtccttg agctcaggca ctcttcttcg cctgggcgag gagcttcgac acggtcggcg
     7981 acatgtgggc gccgcggctc acccagtact gggcgcgctc ggcgtcgatg gtcacctgtg
     8041 ccgggttctt ggtcggatcg tagtggccga tctcctccac cgccgacgcc gtcggggtgt
     8101 tgcgggagtc cgagaccacc acgcggtaga aggggttgtt gcgggcgccc atgcggcgca
     8161 gtcgaatctt gagcattcct gggtctccaa cagggtgttc gcccgcccgg ccacacgtcc
     8221 atccacggcc ggctggcgca aagacggggg attgtatcag ggacggcgcg cttctccaac
     8281 tcgggctgct gcgggtccta ctgcgggggc atggccgcgc cgccgcccag ggccttgcgc
     8341 agccacttgc cctggacccc cttgagcatc ttcttcatgc tcttgtgctg cttgagcagc
     8401 cggttgatct cctgcaccga ggtgccggac cccatggcga tgcgcttctt gcggctgccg
     8461 ttgatgacgt cgggccgctg gcgctcgagc ggggtcatgg agttgatgat cgcctcgacg
     8521 cggctcaggc cgctctcgtc gacgtcggcg ccctcgaggc cgcgcagcgg ccccaccttg
     8581 ggcagcagct ccagcacctg gcccaggggg ccgaggcggc ggatctggcg gagctggtcg
     8641 cgcaggtcct ccagggtgaa ctcctggcgg gcgatgcggc cggcgaggcg cttcgcctcc
     8701 tcctggtcga tcccctgctc cgcccgctcg atcagcgaca ggacgtcgcc catgccgatc
     8761 atgcgggagg acagccgctc gggatggaac agctccaggt cgtcggactt ctcgcccatg
     8821 ccgacgaagc ggatgggcac tcgggtgacg gtgcggatcg agagggcggc gccgccgcgg
     8881 gagtcgccgt cgagcttggt gaggacgacg ccggtcagcg gcagggtctg gccgaactgc
     8941 tcggcgctct tgaccgcgtc ctggccggtc atcgagtcgg cgacgaacag gacctcgtgc
     9001 ggctggatgc ggtcggcgag gcgcttgatc tcctccatca gggcggcgtc gacgtgcagg
     9061 cggccggcgg tgtccatgat caccacgtcg tggccgcgct ggcgggcgac gttgagagcc
     9121 cgcgcgccga cgtcgatcac ggactcgccc ggctcgggct cgacgaccgg cacctcgacc
     9181 cccttgccga cctgcttgag ctgctcgacg gcggcagcgc gctgcaggtc gccggcgacg
     9241 aggagcgggt gcttgccgcg cttcttgagg cgcttggcga tcttgccggc ggtggtggtc
     9301 ttgccggagc cctggaggcc gcagagcatg atcacggccg gccggccgtc gaggcgcagc
     9361 tcggtcccct cctcgccgag gagcgaggtg agctcgtcgc ggacgatctt gaccacctgc
     9421 tgggcgggcg tgaggctctg cagcacctcc tggccgaggg cgcgctcgcg cacccgctcg
     9481 atgaagggct tgaccacccg caggtggacg tccgcttcga ggagcgccag gcggatctgg
     9541 cgcaacgccg cctgcaggct ctcctcggtg accttgccct cgcccttcag attgcggaag
     9601 acgtcctgga gcttcgtctg gagaccgtcg aacatctgga cctcggtgtc tggatgaatc
     9661 gggagccgcg gccgccgcgg cgtcggggtt gaaaatggta ccacgggacc ccggagggcc
     9721 cgtgagccga gaacacgggg aggaaggggg aggattcctg cggatcccat ccatgcgggc
     9781 cggagacgat ccggaacggc gggaagcttc ccggagcgtc cggatgcctt ccaggcgatc
     9841 tggacgccgc ccgaagcgtc cgggacgcct ccccggccgc ccggacgagg tccagagcgt
     9901 ccggacgaaa tccggggagt ctggacgaga tccagggcgt ctggatgggt tcccaggcgg
     9961 agaggattct tactcgaggc cgagatcgcg cagagagccc accaccagga tctgatccgc
    10021 catcgcttcg agcttccgga cggaggagat ggctccgacc cgctgccgga cctccggcgc
    10081 caggggaccg aagcgccgct ccagctgacg cagcaggaga ttccgggcgc cttcgacccg
    10141 cccctttctc tcccctgctt tgtggccttc ctgtctcgcc cttcgccgat cttctctcgc
    10201 ccagcggatt aggttctctt cgagcatcgg aatctcctcc aagtcggctc tgtccgggat
    10261 tacggctccc ggatgggccc ggcgcaacgc cctcagcagc cagcccgtga acgcctgccg
    10321 cagctccggc tcccgatccc gaggcaactg agccgagagc tcggccgcca ccgccaggat
    10381 ctcctccaga gaatggctga cctccagccg gatcagcgcc acggccaggt tcgacgcgtc
    10441 ccccagctct ccgggagcaa tccgcctctc gtccagcacc gtgtaggtga accgcgggca
    10501 cgaacgtccc agcgtccccg gggccggctc gatcaggctc tcgaggtccc gtgccgcgtt
    10561 ccagcgccgg ttcccattat agatgaccat caccagcacc ggcggcaact tgcccgacgg
    10621 tgtcagggcg cggttgcgga tgatgtcctc gtagagcagc gacaagtacg tcaagaggcg
    10681 caccgccatg aagcgctccg gcgtcgcctg gaattcgagc aacaggtaca ccggcgtcgg
    10741 ggcggcgccg gggcgcttcc agagcaggcg ccagaccacg tcgttgtgcc ggctggtcaa
    10801 gtcgccgctc atgaagtcgc cgctgacccg cgccagcgtc gcgaagtcga gctgcctcac
    10861 ccagggctcc cgcacgaacc tgcgcagcag gtcggcgacg acccgcgcgt gagcgaagag
    10921 ctgcttgtag ccactgtcgt gccttcccat cctccctcca ttccggtgtg aaccgcgtcc
    10981 ggaaggagga aagacggaat ccggagaaaa aatcgctcaa ctttaagcat ccctaaagat
    11041 cgagcgggcc cagagccgcc gatccgcacc gcccgctata ctctccccca tggaacgacg
    11101 ggcgaagatc atggccacct tggggccggc gagcactcag gaggatgtcc tccaggagct
    11161 catccatgcc ggcgtagacc tcatccgcct caacctctcc catggcacgc aggacgacca
    11221 ccgcaagacc ctccacctgg tgcggcgcgt cgccgagcag gagggttgcc atctccccgt
    11281 agtcatggac ctcatgggcc cccgctatcg gctgggcaag atcccggggg agcgccggat
    11341 gctcgagccg ggcgaggagc tcgtcctggg tacccagtcc gaaggggtcg acctgccggt
    11401 ggactccgag atcctgagcc acctcgaagc cggcgagcgc gtgctcatcg acagcggcct
    11461 tatcgagctg aaggtggagt ccaaggaagg agaccgggtg tcggcgcggg tggtcaacgg
    11521 cggcccggtc cgcacccgca aggggatcaa cctgcccgat acctccctgc ccttcgagat
    11581 ctcggagaag gaccgctccg acatcgcctt cgccgtcgcc gagggtgccg actacctggc
    11641 cgcgagctat gtcggcgagg cctcccacat cgaggcgctg cgcgaggtcg tcacccaggc
    11701 cggcgggcgc atccccatcg tcgccaagct ggaacgggcg accgccatgg agcacctgga
    11761 cgagatcacc gaggcggccg acggcctgat ggtcgcccgc ggcgacctgg gggtcgaggt
    11821 gccgctccac accgtgccgg tgcaccagaa gcggatcgtc gccgccggcc ggcgggcggg
    11881 caagccggtc atcgtcgcca cccagatgct ggagtcgatg atcgagcagc cgcgccccac
    11941 ccgcgccgag gccaccgacg tcgccaacgc cgtgctcgac ggcgccgacg cgctcatgct
    12001 ctccggcgag accgccatgg gcaagtaccc ggtgggtgcc gtctccacca tggcccgcat
    12061 catcctggaa gccgaggagt acgcccgcga gtgcgccacg gacgagcgcc cctcgatcca
    12121 ccccctgggg ccggtccacg cccagcgcac cttcgccctc gacccctcgg cttccgacga
    12181 gggctccgag tccaccctgg acgtccccga cctggtctcc gccgccgccg tctacgccgc
    12241 cgaccagctc gaggtgcggc gcatcgtcgc cttcagccag agcggcttca ccgcccgcct
    12301 ggtcgcccgc taccggcccg acgctccggt ggtcgccttc acccccgacg aacgggtggc
    12361 gcgccagctc cagctcgtct ggggcgtccg gccgctcctc gccgacatcg aggtcggcac
    12421 gctcgacgaa gtggtacagc tcgtgcagcg ccagctcctc gccgcccggc tggtcgagcc
    12481 cggcgagcgg atcatcattc tcatgggaca ccccatccgc gaccggccgc tgaccaacct
    12541 gatgcgggtc caccgcatcc gggcggtctg agggaagaga aagagagaag agaccaggcc
    12601 ccttgctccc cctgcacgac atcaacccac ggcggagctt cccggcggtg acggtggtgc
    12661 tgatcgccgt caacgtcctg ctgttcttct atgagctctc cctggggccc cacctggagg
    12721 cgttcatgct gtcgtcggcc ttcgtgccgg cccgcctctt cgatgacggc gcgatctatg
    12781 ccagcgagat cggcccggag gcacggagcg ccctgctctc catgttcctg cacggcggct
    12841 gggggcacct gctgggcaac atgctcttcc tctgggtctt cggcgacaac atcgaggacc
    12901 ggctg
//
LOCUS       DASPJI010000107         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_349278, whole genome shotgun sequence.
ACCESSION   DASPJI010000107 DASPJI010000000
VERSION     DASPJI010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349278"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1999
                     /locus_tag="VEL74_02835"
     CDS             <1..1999
                     /locus_tag="VEL74_02835"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYG61491.1"
                     /translation="DRVYTGTLNFMPFLDPPPATAEEIAEVRADVDAAGILYGTLLSR
                     DGKVATVLVGVPPGPPGKVDREGLYRQVLKVASPFATPNDRISVVGAPVAEALLGAHI
                     LEDLRHLLPLAVGVIALAIWIACRRLWGVALAMLKVGAILVVTFGIMGWSGSPVRLTS
                     AMLPVLLTTMSLVDEIHLFFRYQRRLAGGHDGPFPGAVLEAMRQMARPMVLTAITTAI
                     GFFSLLTSPLPAVRSLGIFAGLGIFASLFWALSFTPAALKLLGPARMARPVSSPVAGA
                     WTVRALAPLVRRPLPTLLVLALVTVAGLAGLRLLYVQDSWIDGFAPGSEFRQATERAD
                     AQLLGTHLLLVQLTFTPPKDKPVPAGWVREGPLLDPAALRAIGDFEAYVRGLPRVGGV
                     LGAHSQLTTVAYLWLARREGARSIPDTPERVETLLDRYDMARGEAKRREVLDDGLRRA
                     MVTVFLEHANYRDTARIMEAIRRYERERFRPLGARLDFAGDVAVSQSMIPDIVQTQIG
                     SLLTTMLGAFLAVALFWRSWRAGLWVLAPTSVAILWVLGSMGWLGIPLGVATSMFCAI
                     ALGLGDYAVHFLEEVERAGEAGEAEPVLRGIEVAGPSTVADTLAIGAGFGLLVFSQVP
                     ANARLGGLVAASLAASCVLTLVGMGALLELIRRRRRGQRMA"
     gene            2042..>2285
                     /locus_tag="VEL74_02840"
     CDS             2042..>2285
                     /locus_tag="VEL74_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HYG61492.1"
                     /translation="MGETTEIPNQPPRRRAGSAVLVAAGILLSRVVGLLRDMIVARFF
                     GVGPHADVFRAALRAPNVLQNLLGEGTLSAAFIPIYS"
BASE COUNT          253 a          817 c          844 g          371 t
ORIGIN      
        1 cgaccgggtc tacaccggca ccctgaactt catgccgttc ctcgacccgc cgccggccac
       61 ggcggaggag atcgccgagg tgcgggcgga cgtggacgcc gcgggcatcc tctacggcac
      121 cctgctctcc agggacggca aggtggcgac ggtgctggta ggggtgcccc ctggtccgcc
      181 ggggaaggtg gaccgcgagg ggctctatcg ccaggtgctg aaggtggcga gccccttcgc
      241 cacgccgaac gaccggatct cggtggtggg ggcgccggtg gcggaggcgc tgctgggtgc
      301 ccacatcctg gaggacctgc ggcatctcct ccccctggcc gtaggggtga tcgccctggc
      361 catctggatc gcctgccgcc gcctgtgggg cgtggccctc gccatgctca aggtcggcgc
      421 gatcctggtg gtgaccttcg ggatcatggg ctggagcggc tcgccggtcc ggctcacctc
      481 ggcgatgttg ccggtcctgc tgacgaccat gagcctggtg gacgagatcc acctcttctt
      541 cagatatcag cgccggctgg cggggggcca cgacggcccg ttccccggtg ccgttctgga
      601 ggccatgcgg cagatggccc ggccgatggt gctcaccgcc atcaccacgg ccatcggctt
      661 cttctccctg ctcacctcgc cgctgccggc ggtccgctcg ctcgggatct tcgccggcct
      721 ggggatcttc gcgtccctgt tctgggcgct gagcttcacc ccggcggcgc tcaagctcct
      781 ggggccggcg cggatggcgc ggccggtctc ctcgccggtg gcgggggcgt ggacggtgcg
      841 ggcgctggcg ccgctggtcc gccgccccct gcccaccctg ctggtcctgg ccctggtgac
      901 cgtggcgggg ctcgccgggc tgcggctgct ctacgtgcag gacagttgga tcgacggctt
      961 cgccccgggg agcgagttcc gtcaggccac cgagcgggcg gacgcgcagc tcctgggcac
     1021 ccacctgctc ctggtgcagc tcaccttcac tccgcccaag gacaagccgg tccccgccgg
     1081 ctgggtgcgc gaggggccgc tcctcgaccc ggcggcgctg cgtgccatcg gcgacttcga
     1141 agcctacgtc cgcggcctgc cccgggtggg cggcgtgctg ggggcgcaca gccagctcac
     1201 caccgtcgcc tacctttggc tggcgcggcg ggagggcgcg cgctccatcc ctgacacccc
     1261 ggagcgggtg gagaccctgc tcgaccgcta cgacatggcg cggggggagg ccaagcgccg
     1321 cgaggtcctc gacgacggcc tgcgccgcgc catggtcacg gtcttcctcg agcacgccaa
     1381 ctaccgggac acggcgcgga tcatggaggc gatccggcgc tacgagcgcg agcgcttccg
     1441 gccgctgggg gcgcgcctcg acttcgcggg cgacgtggcg gtctcccaga gcatgatccc
     1501 cgacatcgtc cagacgcaga tcgggtcgct cctcaccacc atgctcggcg ccttcctggc
     1561 cgtggccctg ttctggcggt cgtggcgggc ggggctctgg gtgctcgcgc ccacgtcggt
     1621 ggcgatcctc tgggtgctgg gcagcatggg ctggctcggt atcccgctgg gggtggccac
     1681 ctccatgttc tgcgccatcg ccctgggttt gggggactac gccgtccact tcctggagga
     1741 ggtggagcgg gccggggagg cgggggaggc cgagccggtg ctccgcggca tcgaggtggc
     1801 gggcccgtcg acggtggccg acaccctggc catcggtgcc ggtttcgggt tgctggtctt
     1861 ctcccaggtg ccggccaacg cgcggctcgg cgggctggtg gccgcgtcgc tggcggcgag
     1921 ctgtgttctc accctggtgg ggatgggcgc gctcctcgag ctgatccgcc gccgccgtcg
     1981 cggccagcgc atggcgtaga atcccgtaaa atcacgggat cggcggccgg ctgcgaggag
     2041 catgggagag accacagaga taccaaacca gcctcccaga cggcgcgccg gcagcgccgt
     2101 cctggtcgcc gcgggcatcc tgctgtcccg ggtcgtcggc ctgctgcggg acatgatcgt
     2161 ggcgcggttc ttcggggtgg ggccgcacgc ggacgtcttc cgggccgccc tgcgcgcccc
     2221 caacgtcctc cagaacctgc tcggcgaggg gacgctgtcc gccgccttca tcccgatcta
     2281 ctccc
//
LOCUS       DASPJI010000108         3163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_238498, whole genome shotgun sequence.
ACCESSION   DASPJI010000108 DASPJI010000000
VERSION     DASPJI010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238498"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1035
                     /gene="speB"
                     /locus_tag="VEL74_02845"
     CDS             151..1035
                     /gene="speB"
                     /locus_tag="VEL74_02845"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HYG61493.1"
                     /translation="MPSLPTNFGLLDADASDYETSRVAVLPVPFERTTSYGKGTAAGP
                     AGIIRASQAMELWDEELQGEPYLQGIATLPPFLPEVFDMGEALAELQEEARVHLDRGK
                     FLVTLGGEHSLSSAPVKATRDVYGEIGVVQFDAHADLREEFDGTPYSHASVMKRIVDE
                     GIPTLPVGIRSLSSPEAELIRERGMRVIWGHDLDQAGQLFPRLLEALPEKIYLTFDID
                     FFDPSLVPATGTPEPGGGLWYPTLRLLRRLFQEKTVVAMDVVELAPIGGQPASDFLTS
                     KLIYKCLGYLWEKEGRKS"
     gene            complement(1039..2259)
                     /locus_tag="VEL74_02850"
     CDS             complement(1039..2259)
                     /locus_tag="VEL74_02850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYG61494.1"
                     /translation="MSSSVSGPPSLWLARPSAPEEVDVAVVGAGIAGLSTAWWLARAG
                     RRPVVLEAGGVACRASGRNAGFLITGTAEPYTIVARQMGETAALRYWELSRDNRELLR
                     AELLEPGRVDCDFTPEGSWTAAVSGRPHQEQAMRESAEILAGLGFDLEWREASEVRRA
                     SGCDRLAGAIFQPRDAGLHSVRLCRGIARLIEAAGGEVRTGFRVRALESAGDRVRVIS
                     DAGQVIAERVVLTLNAYAPALLPHLAGEVRPVRGQILATAPAPRDLPGVWYVNDGFEY
                     LRQLPDGTFLLGGCRWCAVDAEVGYLEAPTATVQGALEGFLRDYFPRFAHLPVVHRWA
                     GTMAFTPDGLPRTGEVPGIPGASYFAGFNGHGMSLGFVTGRWLADRAMGKEPAPLFPP
                     AASRTDLHLGPAPV"
     gene            complement(2243..2998)
                     /locus_tag="VEL74_02855"
     CDS             complement(2243..2998)
                     /locus_tag="VEL74_02855"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HYG61495.1"
                     /translation="MIDLTGRRVLVTGGSRGIGAACCRLFARAGATVMIQYLASGERA
                     RALYAELLRTSGRPHLKARCDVSDPEQIEKLFEQVRQEWGGLDCLVNNAGVWVYDPLV
                     KLDRDQLQATLAVNVVGPFLCSREAVPLLQESEDGTIINVSSTAGQRGEAYYSPYAAS
                     KGAMISATKSWSTELAPKIRVNSVAPGWVDTDMTAEALSGERRQQILESIPLHRIASP
                     EDIAGSVVFLASPLARHITGEILNVNGGSVLVG"
     gene            complement(2995..>3163)
                     /locus_tag="VEL74_02860"
     CDS             complement(2995..>3163)
                     /locus_tag="VEL74_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013299367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HYG61496.1"
                     /translation="VNGGAIALGHPLGASGTRILITLLKELKRRGGGRGVASACIGGG
                     QGIAMVVEAAA"
BASE COUNT          474 a         1176 c         1078 g          435 t
ORIGIN      
        1 cgctcgcgga cctcaccgga aagctccaca ggctccatgg ccatcctcct cctcggtctt
       61 cgtcctcgcg ttgacactcc cggacgcatc ctgctacaaa ctccccgctt ccgggaactc
      121 ccccgaccgc tcttcaggag atctctcccg atgccctctc tgcccaccaa cttcggcctg
      181 ctggacgccg atgcgagcga ttacgagacc agccgcgtcg ccgtgctccc cgtccccttc
      241 gagcgtacaa cctcctatgg caaggggacc gcggccggcc cggcgggcat catccgcgcc
      301 tcccaggcca tggagctgtg ggacgaggag ctgcagggcg agccctacct gcaggggatc
      361 gccaccctgc cgccattcct ccccgaggtc ttcgacatgg gcgaggccct ggccgagctc
      421 caggaggagg cccgcgtcca tctcgaccgc ggcaagttcc tggtgacgct gggcggcgag
      481 cacagcctct cgtccgcgcc ggtcaaggcg acgcgcgatg tctatggcga gatcggggtg
      541 gtccagttcg acgcccacgc cgacctgcgg gaggagttcg acgggacccc ctacagccac
      601 gccagcgtca tgaagcggat cgtcgacgag ggcatcccca cgcttccggt gggcatccgc
      661 tcgctctcct cgccggaggc cgagctgatc cgcgagcgcg gcatgcgggt gatctggggc
      721 cacgacctcg accaggcggg gcagctcttc ccccgcctcc tcgaagccct gccggagaag
      781 atctatctca ccttcgacat cgacttcttc gacccctcgc tcgtaccggc caccggcact
      841 cccgagccgg gcggcggcct ctggtacccg acgctccgcc tcctccgccg cctcttccag
      901 gagaagacgg tcgtggccat ggacgtggtc gagctggcgc cgatcggcgg ccagccggcg
      961 agcgacttcc tgacctcgaa gctgatctac aagtgtctgg ggtatctctg ggagaaggaa
     1021 ggccggaaga gctgacccct acacgggagc cgggccgagg tggagatcgg tccggctcgc
     1081 ggcaggtggg aagagcggcg ccggctcctt ccccatcgcc cgatcggcca gccagcggcc
     1141 ggtgacgaaa cccagcgaca tgccgtggcc gttgaagccc gcgaagtagg aggcgccggg
     1201 tatgccgggc acctcccctg tccgcggcag gccgtccggc gtgaaggcca tggtccccgc
     1261 ccagcggtgg acgaccggaa ggtgggcgaa gcgcgggaag tagtcgcgga ggaacccctc
     1321 cagagcgccc tggacggtgg ccgtgggcgc ctccagatat cccacctcgg cgtcgaccgc
     1381 gcaccagcgg cagccgccga ggagaaaggt gccgtccggg agctggcgca ggtattcgaa
     1441 gccgtcgttc acgtaccaga cccccggcag gtcgcgcggc gcgggggcgg tggcgaggat
     1501 ctggccgcgg actggccgga cctcccccgc caggtgcggc aacaaggccg gcgcgtaggc
     1561 gttgagcgtc agcacgaccc tttccgcgat cacctgaccg gcgtcggaga tgacgcggac
     1621 gcgatccccc gccgactcca gcgcccgcac ccggaagccg gtgcgcacct caccgcccgc
     1681 cgcctcgatc aggcgggcga tgccgcggca gaggcgcacc gaatggaggc cggcatcccg
     1741 cggctggaag atggcacccg ccagccggtc gcaaccactg gcccgccgca cctcagaggc
     1801 ctcccgccac tcgaggtcga agcccaggcc ggccaggatc tccgcgctct cccgcatcgc
     1861 ctgctcctga tgcggccggc cggacaccgc ggcggtccag ctcccctccg gcgtgaagtc
     1921 gcagtccacc ctgccgggct ccaggagctc cgcccgcagc aactcgcggt tgtcccgcga
     1981 cagctcccag taacgcaggg ccgccgtctc ccccatctgc cgcgccacga tcgtgtaagg
     2041 ctcggccgtg ccggtgatca ggaagcccgc attccggccc gatgcccggc aggccacgcc
     2101 gccggcttcg agcacgaccg gccgccggcc cgcccgagcg agccaccagg ccgtcgacag
     2161 cccggcgatt cccgcgccga cgacggcgac gtccacctcc tcgggtgccg aggggcgggc
     2221 gagccagagg gatggggggc cgctaaccga cgaggacact gccgccgttc acgttcagga
     2281 tctcgcccgt gatgtggcgg gcgagcgggg aggcgaggaa caccacggag cccgcgatgt
     2341 cctcggggct cgcgatgcgg tggaggggga tcgattccag gatctgctgc cggcgctccc
     2401 ccgacagcgc ctcggcggtc atgtcggtgt ccacccagcc cggggccacg gagttgaccc
     2461 ggatcttcgg cgccagctcg gtggaccacg acttggtggc cgagatcatc gcccccttgc
     2521 tcgcggcata ggggctgtag tacgcctccc cgcgctggcc ggcggtggag ctgacgttga
     2581 tgatcgtacc gtcctcggac tcctggagca gggggaccgc ctcgcgcgag cagaggaacg
     2641 gcccgaccac gttgaccgcc agtgtcgcct ggagctggtc gcggtcgagc ttgaccaatg
     2701 gatcgtagac ccagacgccg gcgttgttga ccaggcagtc gagcccgccc cactcctgcc
     2761 gcacctgctc gaagagcttc tcgatctgct cgggatcgct gacgtcgcaa cgggctttga
     2821 ggtgggggcg gccggaggtg cgcagcagct ccgcgtagag ggcgcgggcc cgctcgccgc
     2881 tcgccaggta ctggatcatc accgtggcgc cggcccgggc gaacagccgg cagcacgcgg
     2941 cgccaatgcc gcggctgcca cccgtcacca ggacccggcg ccccgtgagg tcgatcatgc
     3001 ggcggcctcg accaccatgg cgatgccctg accgccgccg atgcaggccg aggcgacacc
     3061 gcgtccgccg ccccgccgct tgagctcctt gaggagcgtg atcaggatgc gtgtgcccga
     3121 ggcgcccagg gggtggccca gggcgatggc gccgccgttg acg
//
LOCUS       DASPJI010000109        10265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_296172, whole genome shotgun sequence.
ACCESSION   DASPJI010000109 DASPJI010000000
VERSION     DASPJI010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10265)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10265
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296172"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="VEL74_02865"
     CDS             complement(<1..1354)
                     /locus_tag="VEL74_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG61497.1"
                     /translation="MIRHSSAVVLSLLLALPPAALPQEAPRGGDEVLFETVDVSVVNV
                     EVFVTDREGKRVTGLTKDDFELYEDGKPVEVSYFYASEGTPGAAGSAAERPEPERLQL
                     AVYLDVEELPDEARPRIVESIERFVASRAKPGDQVMLASYNGPDTLTVRFVPADRPTF
                     AAALKAVADAPKPAPSNSADQRLLQTKQEMAVQAGPDMVGKDSAAAMAALDRADIAMT
                     AASNQLKFRNLARYSLSALTQFLGGLGSLPGRKALLYVSANATFRPGQTIEQTFDRKY
                     GRNMVLDGPLRKQLNDLEEGASSERILFFAMGARDVLQEVVSGAKADPIVTVAGKIDH
                     AQDLMDAAPDAALAKVVEDLDSYYSLGFTPAKREPGKRHRIEVKMKRPGLRVRHTEEY
                     RELTPNARASNRTVAALLLGSTGRGGAIGGVGVQGAAAREDDNPLGIRLQVGQPEAGK
                     G"
     gene            complement(1351..2943)
                     /locus_tag="VEL74_02870"
     CDS             complement(1351..2943)
                     /locus_tag="VEL74_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG61498.1"
                     /translation="MTRRIRALVLTLLASALPVSAAAPEPGELFADSVDVRLVNIEVF
                     VTGPDGKRVTGLTRDDFEVFEDGRRVEITHFYAAEPAPRASEPLRPAPVPEAERLQLA
                     VYFDTEDLPERAIPRIIHAVEEFVASRARPDLRPDERVIVAHYHGADSLAVREVPVNR
                     PALSAALSEMARVPQGASLSGLVSRFGSRGTSDRAVAEMNTQSEALRRRGQAKSAFDA
                     LDRFVTALGSLPGRKALVYVSGKVSFSPGQSLFTVFENKLGLSVRQGAMRPLVKRLED
                     TVNSDRIAFYGLGARNTRATSISAGAYEPKRLEIPKPGEDIVDIGRDLIDANPAASLA
                     WVGEDLDSYYSLGYTPDRRPDGKRHRVEVRVKRRGLDVRHLESYRDRTPADRAEARTL
                     ASLLLGGGENPLGLIFQFGPAEAAGKEGVMVPLTLRLPLARLALLPGPGGAPEGRLSV
                     VITVRDDQGRMAEPTRLTLPVRPGGETPAGQQMSFTTPLLMRPIPHTVVIGVRDELGN
                     TLSTVTAEYNPATGRPLEDDPK"
     gene            complement(2940..4571)
                     /locus_tag="VEL74_02875"
     CDS             complement(2940..4571)
                     /locus_tag="VEL74_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG61499.1"
                     /translation="MTSRPRALALALLAASLCAHPAPAQAPAPPQPPQGSEVFADSID
                     VNVVNIDVSVTDRKGKWVTGLTRDDFEVFEDGKPVEITYFYAAEAAAEARPEAPVPAV
                     PAPERLQFAVYFDLESLPKQARPKVAAAVEEFLVSRASPQETVLLATYNGPDTLNVHE
                     LPASRPVLAAALQEIVQAGRLAPVSEQEQRLTAMTDNTIDLGGNEELDKAIYESNAAQ
                     AELDSIVASLMKRIQTRATLTALQGFVANFGSRPGRKALVFVSGGITFRPGQTIVQAF
                     ETKLGPSAREGPLSFLVRDVELAASSDRIAFYALGGSKSIYRPSISTRSNTPPPAPQI
                     IDRSREIVDANPAASLAWIGEDLSSYYSLGYTPPERQPGRRHRVEVKVKRRGLHVRHI
                     ERYRERAPVEQARIRTAATLQMGGGENPVGMSVQMGQTGPEERGKVLVPVTLEIPLSK
                     VALLPGEGGAQHGNLSILITARDSKGRIAAASDTELPLRFDGPPPPGVSYSFQLRLRS
                     APHKIVLGVWDEVGNTVSTVTSHYAPEARAGRPSP"
     gene            complement(4568..6175)
                     /locus_tag="VEL74_02880"
     CDS             complement(4568..6175)
                     /locus_tag="VEL74_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG61500.1"
                     /translation="MMSRFRALAPALLMLSLLVPSLAASPQEPAPPQPSHGEEVFADS
                     IDVNVVNIEVAVTDRKGKWVSGLTREDFEVYEDGEPVEITYFYASEKTGAVPAPERLQ
                     LAVYFDLESLPAISRPPLSAAVEEFLVSRAGPQEKVLLAAYDGPDTLDVREVPASRPA
                     LSAALQEILRPGRRFPASVLEARMTAMSGSAVALGGSGPLDLAMAQADAEVTSFDVIV
                     ARLVRRMKQRSALEALEGFVASLGSRPGRKALVFVSSGITFRPGQSVVQAFETKLGDQ
                     AKAGPLSFLVRDVELAASSDRIAFYALGAPGRMARPSISARKNTSSGAPVIDRSREIT
                     DGAPTASLAWIGEDLSSYYSLGYTPATRQPGKRHRVEVKVKQRGLHVRHIDRYRERTP
                     GEHARNRTAAALQMGGGENPLGMSVQMDPAGPEQKGKVLVPVTVDIPLSQVALLPGEE
                     EARHGRLSILITARDSQGRTAAASDTELPLRFDGPVPPGISYTFQLELRSAPHTIVLG
                     VWDEVGNLLSTVTSEYDPATGSGRAPR"
     gene            complement(6192..7817)
                     /locus_tag="VEL74_02885"
     CDS             complement(6192..7817)
                     /locus_tag="VEL74_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG61501.1"
                     /translation="MARRARALALTLLAVPLCIHPLAASAQEPAPPQSPPGEIFADSI
                     DVNAVNLEVVVTGRDGERVTGLTRDDFEVYEDGRPVEVTYFHATAGPARPADEIPAPE
                     RLQLAVYFDLEDLPEKSRPGLAGAVEDFLVSRAGPEEKVLLASYNGADTLNVRELPAS
                     RPALAAALREIVETPRLSPATQQERQFTALSTQMIGSLGGSNALDLAMIEEEKAVARF
                     DDKGREMRAQMRQRSSLAALEGFVASLGGQPGRKALLYVSGSIAFRPGQSLAEAFETK
                     LGRGIAKPDAEGPQPFYFLVQGLQEVAASERIAFYALGTRHSAAPNITSRANTPSAPL
                     VIDRSREITEANPAASLAWIGEDLSSYYALGYTREKGQPGRRHRVEVKVKRPGLHARH
                     IESYVERSPEDRMRSRASAALHQLQRDGGENPLGVSLRMGQAVPEGKKQVIVPLTLEV
                     PLSGITLLPGEGGARHGQLSIFVTARDGKGRTAAVAQTDLPLRLDGPLPPGISYDFQL
                     ALRPGPHEIVLGVWDELGNTLSTLVSRYDPAVQ"
     gene            7959..8795
                     /locus_tag="VEL74_02890"
     CDS             7959..8795
                     /locus_tag="VEL74_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61502.1"
                     /translation="MSRPLAAAVLALLLAAAAGCHAQPTQPARPPFDSEMVALPLGES
                     EVRLAVHTAGQGPSFLVLHDSEDTAAEVGLEAVRARGGRLIEVQSEGRRRVHFEHRGR
                     TWSFDPNRIFTDGGAPWTLREHGGEPPPEVVAEVRRFAEALVGVHGPGSYPVLITLHN
                     NTEGDYSAASYLPGGSRDGHAAALHLPDGADPDDFFFVTDRAIYDALVQEGFPVVLQD
                     PERVEDDGSLSVWAARRGIPYVNVEAQHWHYGQQKRMLEALSRVLDAHDAVRDAAGTL
                     TR"
     gene            8854..9063
                     /locus_tag="VEL74_02895"
     CDS             8854..9063
                     /locus_tag="VEL74_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61503.1"
                     /translation="MKKLALALVLCAGALATGGPLMAHHGGCHPNPRALCPDVYAPVT
                     CSNGVTYSNSCRASAACATGCVGGV"
     gene            9413..9769
                     /locus_tag="VEL74_02900"
     CDS             9413..9769
                     /locus_tag="VEL74_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61504.1"
                     /translation="MPSFSDRTPSRVDLSRPTESTRRHLYELVVHVYFHPTPLEVEQA
                     RQDPGNHWVPDYGPDEARLHVFYAYGRWFAAWEALEERKEAAAESLPAWRRWSVVRIV
                     EDADPSSGTGVTFLEV"
BASE COUNT         1407 a         3507 c         3779 g         1572 t
ORIGIN      
        1 ttcccttccc ggcttcgggc tggccgacct ggagccggat gcccagcggg ttgtcgtcct
       61 cccgcgccgc cgcgccctgc acgccgacgc cgccgatagc gccgccccgt ccggtggagc
      121 cgaggagcag cgccgccacc gtccggttcg aggcgcgggc gttcggggtc agctcccggt
      181 actcctcggt gtggcggacc cggagccccg ggcgcttcat cttgacctcg atgcggtgcc
      241 gcttgcccgg ttcccgcttc gcgggggtga agccgaggga gtagtaggag tcgaggtcct
      301 ccaccacctt ggcgagcgcc gcgtccggcg cggcgtccat caggtcctgg gcgtggtcga
      361 tcttgccggc gacggtcacg atcgggtcgg ccttggcgcc ggagacgacc tcctggagga
      421 catcccgcgc gcccatggcg aagaagagga tgcgctcgga gctggcaccc tcctccaggt
      481 cgttcagctg cttgcggagc ggtccgtcca gcaccatgtt ccggccgtat tttcggtcga
      541 acgtctgctc gatcgtctgg ccgggccgga aggtggcgtt ggcgctgaca tagagcagcg
      601 ccttgcgtcc gggcaggctg cccaggccgc cgaggaactg ggtgagcgcc gagagggagt
      661 agcgggccag gttgcggaac ttgagctggt tcgaagcggc ggtcatggcg atgtccgccc
      721 ggtccagggc ggccatcgcg gccgcgctgt ccttccctac catgtccggg cccgcctgga
      781 ccgccatctc ctgcttggtc tggagcagcc gctgatcggc cgagttggac ggcgcgggct
      841 tgggggcgtc cgccaccgcc ttgagggccg cggcgaaggt cggccggtcg gcgggcacga
      901 agcgcacggt gagggtgtcc ggcccgttgt agctcgccag catcacctgg tcgcccggct
      961 tggcgcggga ggcgacgaag cgctcgatcg actctacgat ccgcggccgg gcctcgtcgg
     1021 gcagctcttc gacgtcgagg tagacggcga gctgtaggcg ctcgggctcg gggcgctcgg
     1081 cagcgctccc ggcggcgccc ggcgtccctt ccgaggcata gaagtaggag acctccaccg
     1141 gcttgccatc ctcgtacagc tcgaaatcgt ccttggtgag ccccgtcacc cgctttcctt
     1201 cgcggtcggt gacgaacacc tcgacgttga cgacgctgac gtcgacggtc tcgaacagga
     1261 cctcgtcgcc gccgcgcggg gcctcctggg ggagggccgc cggcgggagg gcgaggagaa
     1321 gggacaggac taccgcggag ctgtgtcgga tcatttcgga tcatcctcca gggggcggcc
     1381 ggttgccgga ttgtactcgg cggtcacggt ggaaagcgtg ttccccagct cgtcgcgcac
     1441 accgatgacg actgtgtggg ggatcggccg catcagcagc ggggtggtga acgacatctg
     1501 ctgaccggcc ggggtctcgc cgccgggccg gaccggcagg gtgaggcggg tgggctccgc
     1561 catcctcccc tggtcgtcgc gcacggtgat gacgaccgag agccgcccct cgggagcgcc
     1621 gccgggcccc ggcagcaggg ccagccgcgc gagggggagg cgcagggtga gcggcaccat
     1681 caccccttcc ttcccggcag cttcggccgg accgaattgg aagatcaggc ccagggggtt
     1741 ctccccgcca ccgaggagca gggaggccag ggtgcgggcc tcggcccggt ccgccggggt
     1801 gcggtcgcgg taggactcca ggtggcggac gtcgagcccc cggcgcttca cccgcacctc
     1861 gacgcggtgc cgcttgccgt ccggccgccg gtccggcgtg tagccgaggg agtaatagga
     1921 gtcgaggtcc tcgccgaccc aggcgaggga ggccgcgggg ttggcgtcga tcaggtcgcg
     1981 gccgatgtcc acgatgtcct cacccggttt cgggatttcg aggcgcttgg gctcataggc
     2041 cccggcggag atgctggtcg cccgggtgtt ccgggcgccg aggccgtaga aggcgatgcg
     2101 gtccgagttg acggtgtcct ccagccgctt gaccaggggg cgcatcgctc cctgccggac
     2161 gctgagcccc agcttgttct cgaagacggt gaacagggac tgccccgggc tgaaggagac
     2221 cttgccgctg acgtagacca gcgccttgcg gccgggcagg ctgccgagcg ccgtaacgaa
     2281 ccggtcgagg gcgtcgaaag cggacttggc ctggccccgg cgccgcagcg cctccgactg
     2341 cgtgttcatc tcggccacgg ccctgtcgga ggttcctctg gagccgaaac ggctcaccag
     2401 gccggacagg ctggcgccct gcggcacccg cgccatctcg gagagcgccg cggagagcgc
     2461 cggacggttc accggcacct cgcggacggc gagcgagtcg gcgccgtggt agtgggcgac
     2521 gatcacccgt tcgtccggcc ggaggtcggg ccgcgcgcgg ctggcgacga actcctcgac
     2581 ggcatggatg atccgcggga tggccctctc ggggaggtcc tcggtgtcga agtagacggc
     2641 gagttggaga cgctccgctt ccggcaccgg cgccggccga agcggctcgg aggcacgggg
     2701 cgccggctcc gccgcgtaga agtgggtgat ctccacccgc cggccgtcct cgaagacctc
     2761 gaagtcgtcg cgggtcaggc cggtcacccg cttgccgtcg gggccggtga cgaagacctc
     2821 gatgttgacc aggcggacgt cgacgctgtc ggcgaagagc tcccccggct ccggcgccgc
     2881 cgcggagacc gggagggccg acgccaggag cgtcaggacc agggcacgga ttcgtctcgt
     2941 catggcgaag gcctcccggc tcgggcctcc ggggcgtagt gggaggtgac ggtggacacc
     3001 gtgttcccca cctcgtccca gacgccgagg acgatcttgt gcggagcgga gcggagccgc
     3061 agttggaaac tgtaggagac gccgggcggt ggcggcccgt cgaaccggag cggcagctcg
     3121 gtgtcggagg cggccgcgat cctccccttg ctgtcgcggg cggtgatgag gatcgagagg
     3181 ttcccgtgct gcgctccccc ttcgccgggg agcagcgcga ccttggagag ggggatctcc
     3241 agggtgacgg ggacgagcac cttgcctctc tcctccggcc cggtctggcc catctggacg
     3301 ctcatgccga ccgggttctc ccccccgccc atctggaggg tggcagccgt ccggatgcgc
     3361 gcctgctcca cgggagcccg ctcgcggtac ctctcgatgt gccggacgtg caggccgcgc
     3421 cgcttcacct tcacctccac ccggtgccgc cggcccggct gccgctccgg cggggtatac
     3481 ccgagcgagt agtaggagga gaggtcctcg ccgatccagg ccagcgaggc ggcggggttg
     3541 gcgtcgacga tctcgcggga gcggtcgatg atctggggtg ccggcggcgg tgtgttgctc
     3601 cgggtggaga tgctcggccg gtagatgctc ttggagccgc ccaaggcata gaaggcgatg
     3661 cggtcggagc tcgccgccag ctccacgtcc cggaccagga acgacagcgg tccctccctg
     3721 gcgctcggtc cgagcttcgt ctcgaacgcc tggacgatgg tctggcccgg ccggaaggtg
     3781 atgccgccgc tgacgaagac cagcgccttg cgtcccggcc ggctgccgaa gttggcgacg
     3841 aacccctgaa gggccgtcag cgtggcccgc gtctggatcc gtttcatcag gctggcaacg
     3901 atcgagtcga gctcggcctg ggccgcgttc gactcgtaga tggccttgtc gagctcttcg
     3961 ttgccgccca ggtcgatcgt gttgtcggtc atcgcggtca gccgttgctc ctgctcgctc
     4021 accggggcga gccgcccggc ctgcacgatc tcctggagag ccgcggcgag gacggggcgg
     4081 ctcgccggca gctcgtggac gttcagggtg tccgggccgt tgtaggtggc cagcagcacc
     4141 gtctcctgcg ggctggcgcg ggagacgagg aactcctcga cggccgcggc caccttcggc
     4201 cgcgcctgct tggggagact ttcgaggtcg aagtagacgg cgaactggag ccgctccggc
     4261 gccggcactg ccggcaccgg ggcctccggc cgcgcctccg ccgccgcctc cgcggcatag
     4321 aagtaggtga tctccaccgg cttgccgtcc tcgaagacct cgaagtcgtc gcgggtgagg
     4381 ccggtgaccc acttgccctt ccggtcggtg acggagacgt cgatgttgac gacgttgacg
     4441 tcgatgctgt cggcgaagac ctcgctgccc tggggtggct ggggcggcgc gggcgcctgg
     4501 gccggcgccg ggtgggcaca gagggaagct gccaggagcg ccagagccag ggcgcggggc
     4561 cggctcgtca tcgcggagcc ctcccggacc cggtcgccgg gtcgtactcg gaggtgacgg
     4621 tggagaggag gttgccgacc tcgtcccaga cgccgaggac gatggtgtgc ggggcggagc
     4681 ggagctccag ttggaaagtg taggagatgc cgggggggac gggtccgtcg aacctcagcg
     4741 gcagctcggt gtcggaggcg gccgccgtcc tcccctggct gtcgcgggcg gtgatgagga
     4801 tggagaggcg cccgtgccgc gcctcctctt cgccggggag gagcgcgacc tgggagaggg
     4861 ggatgtcgac ggtgacgggg acgagcacct tgcctttctg ctccggcccg gccgggtcca
     4921 tctggacgct catgccgagc gggttctccc ctccgcccat ctgcagggcg gcggccgtcc
     4981 ggttgcgcgc gtgctccccg ggagtccgct cgcggtacct gtcgatgtgc cggacgtgca
     5041 ggccgcgctg cttcaccttc acctccaccc ggtgccgctt gcccggctgc cgcgtcgccg
     5101 gggtgtagcc cagcgagtag taggaggaga ggtcctcgcc gatccaggcc agcgaggcgg
     5161 tgggggcgcc atcggtgatc tcgcgggagc ggtcgatgac cggggcgccc gacgacgtgt
     5221 tcttccgcgc ggagatgctc ggccgggcca tcctcccggg ggcgcccagg gcatagaagg
     5281 cgatgcggtc ggagctcgcc gcgagctcca cgtcccggac caggaacgac agcggtccag
     5341 ccttggcctg gtctccgagc ttcgtctcga aggcctggac gacggactgg cccggccgga
     5401 aggtgatgcc gctgctgacg aagaccagcg ccttgcgtcc gggccggctg ccgaggctgg
     5461 cgacgaaccc ttccagggcc tccagcgcgg accgctgttt catccgcctg accaggcgtg
     5521 ccacgatgac atcgaaagag gtcacttccg cgtccgcttg ggccatggcg aggtcgagcg
     5581 gcccgctgcc gcccagggcg accgcgctgc cgctcatcgc cgtcatccgt gcctccagca
     5641 cgctcgccgg gaagcgccgc cccggccgca ggatctcctg gagggccgcg gagagggcgg
     5701 ggcggctcgc cggcacttcg cggacgtcca gggtgtccgg gccatcgtag gcggccagga
     5761 gcaccttctc ctgcgggccg gcgcgggaga cgaggaattc ctcgacggcg gcggagagcg
     5821 gcgggcggga gatggcgggg aggctttcga ggtcgaagta gacggcgagc tggagccgct
     5881 ccggcgccgg caccgccccc gtcttctccg aggcatagaa ataggtgatc tccaccggct
     5941 caccatcctc gtagacttcg aagtcctcgc gggtcaggcc gctgacccac ttgcccttcc
     6001 ggtcggtgac ggcgacctcg atgttgacga cgttgacgtc gatgctgtcg gcgaagacct
     6061 cctcgccatg ggatggctgg ggtggagcgg gctcctgcgg ggaggccgcc agagacggaa
     6121 cgagaagaga cagcatcaga agcgccggag ccagggcgcg gaatcggctc atcatcgcga
     6181 aagcctccag atcattggac tgccggatcg taccgggaaa ccagggtgga gagggtgttg
     6241 ccgagctcgt cccagacgcc gaggacgatc tcgtgcgggc cggggcggag cgccagttgg
     6301 aagtcgtagg agatgccggg ggggaggggg ccgtccagcc gcagcggcag gtcggtctgg
     6361 gcgacggccg ccgtcctccc cttgccgtcg cgggcggtga cgaagatcga gagctgcccg
     6421 tgccgcgctc ccccctcgcc cgggaggagg gtgatcccgg agagggggac ctccagtgtg
     6481 agggggacga tcacctgctt cttcccctcc gggacggcct ggcccatccg caggctcacg
     6541 ccgagcgggt tctccccccc gtcccgctgg agctgatgga gggcggcgga ggcccggctg
     6601 cgcatccggt cctcggggga ccgctcgacg tagctctcga tatgccgggc gtggaggccc
     6661 ggccgcttca ccttcacctc cacccggtgc cgccggcccg gctgcccctt ttcccgggtg
     6721 tagccgagcg cgtagtagga ggagaggtcc tcgccgatcc aggccagcga ggcggcgggg
     6781 ttggcttcgg tgatctcgcg ggagcggtcg atcaccaggg gagccgaagg ggtgttggcg
     6841 cggctggtga tgttcggcgc cgcggagtgc cgggtgccca gggcatagaa ggcgatccgc
     6901 tcggaggccg ccacctcttg gagcccctgg accaggaagt agaagggctg gggaccctcg
     6961 gcgtccggtt tggcgatgcc cctcccgagc ttcgtctcga acgcctcggc gagcgactgc
     7021 cccggacgga aggcgatgct gccgctgacg tagagcagcg ccttgcgtcc cggctggccg
     7081 ccgaggctgg cgacgaagcc ttcgagggcg gccagcgaag acctctgccg catctgggcc
     7141 ctcatctccc tgcccttgtc gtcgaacctg gccaccgcct tttcctcttc gatcatggcg
     7201 aggtcgaggg cgttgctgcc gcccaggctg ccgatcatct gggtgctgag agcggtgaac
     7261 tgtctctcct gctgggtcgc cggagagagc cgcggcgtct ccacgatctc ccggagcgcc
     7321 gcggccaggg cagggcggct cgccggcagc tcgcggacgt tcagggtgtc cgctccattg
     7381 taggaggcca gcagcacctt ctcctccggg ccggcccggg agacgaggaa gtcctcgacg
     7441 gccccggcga gccccggccg ggacttctcg gggagatcct cgaggtcgaa gtagacggcg
     7501 agctgcagcc gctccggcgc cggtatctca tccgccggcc gcgccgggcc cgccgtggca
     7561 tggaagtagg tgacctccac cggccggccg tcctcgtaga cctcgaagtc gtcgcgggtg
     7621 aggccggtca cccgctcgcc atcgcggccg gtgacgacca cctcgaggtt gacggcgttg
     7681 acgtcgatgc tgtcggcgaa gatctcgccc gggggtgact gggggggcgc cggctcctgc
     7741 gcggaggccg ccagagggtg gatacagagg gggactgcca ggagtgtcag agccagggcg
     7801 cgggcacggc gagccattga acctcctcgg ctctcctgtg accaccaatc actcctactc
     7861 ccaaaggagc cgagggatcg gagccctcgg accgaaagct acaatgcgca aatcctagca
     7921 tggtccgggc gggcacgcgc tctgcaaaaa tattcggcat gtcacgaccg ctcgccgccg
     7981 ccgtcctcgc cctgctgctc gccgccgccg cgggttgcca cgcccagccg acgcagcctg
     8041 cccggccccc gttcgactcg gagatggtcg cgctccccct gggggagtcc gaggtgaggc
     8101 tggcggtgca cacggcgggg caggggccgt cgttcctggt cctccacgac tcggaggaca
     8161 cggcggccga ggtggggctg gaggcggtcc gcgcccgcgg cggccggctg atcgaggtgc
     8221 agtcggaggg acggcgccgc gtccacttcg agcaccgggg gcggacgtgg tccttcgatc
     8281 cgaaccgcat cttcaccgat ggcggggctc cgtggaccct gcgtgagcac ggcggcgagc
     8341 cgccgccgga ggtggtcgcc gaggtccgcc gcttcgccga ggcgctcgtg ggggtgcacg
     8401 gccccggctc gtatccggtc ctgatcaccc tgcacaacaa cacggagggg gactactccg
     8461 ccgccagcta cctgccgggg gggagccgcg acgggcacgc cgccgccctc cacctgcccg
     8521 acggcgccga ccccgacgac ttcttcttcg tgaccgaccg cgcgatctac gacgcgctgg
     8581 tgcaggaggg ctttcccgtc gtgctgcagg accccgagcg ggtggaggac gacggctcgc
     8641 tgtcggtctg ggcggcgcgg cggggcatcc cctacgtcaa cgtggaggcg cagcactggc
     8701 actacgggca gcagaagcgg atgctggagg ccctctccag ggtcctcgat gcccacgacg
     8761 cggtccggga cgccgcgggc acccttacca gatgagacac caagtgcccg ggcctgcctg
     8821 tcgtgggtcc cggggaatga aggagatcag aagatgaaga aactcgccct tgccctggtc
     8881 ctttgcgccg gcgccctcgc caccggcggt cccctgatgg cgcaccacgg cggctgccat
     8941 cccaatccgc gggcgctctg ccccgacgtc tacgcgccgg tgacctgcag caacggggtc
     9001 acctactcga actcgtgccg ggccagcgcg gcctgcgcca ccggctgcgt cggcggcgtg
     9061 taggccctca ccccccggcc cccctctccc gcctcccctt ccccctcccc gggagagggg
     9121 gaggcggggg tttttcacac cgctccgcgc tccacaactt tcccgcaaga gtccctcatt
     9181 tccctcttga cgcgcccccg gtcgcggtat agtctcgggc ggtcgtaagc cgtacaaccc
     9241 gaagggaggt ggcaggggag agaacgaagc cgcagctgta cagcacagcc gtacaggacc
     9301 gctgtgcctc caaggtgagc gcgtgtctca tccgaggctc cagggtccaa gcggccaagg
     9361 gagggagtta gcgctcgcct cccgaggcct agccggacag gagatagacc tcatgcccag
     9421 cttcagtgat cgtacgccct cgcgcgtcga tctgtcgcgt cccaccgaat ccacccgccg
     9481 tcatctctac gagctggtgg tccacgtcta cttccatccc actcctctcg aggtagagca
     9541 ggcccgtcag gaccccggca accattgggt gccggactac ggtcccgacg aggcgcgtct
     9601 gcacgtcttc tacgcctacg gccggtggtt cgcggcctgg gaggcgctgg aggagaggaa
     9661 ggaggcggcg gccgagtcgc tccccgcgtg gcggcgctgg tcggtggtgc ggatcgtcga
     9721 ggacgcggac ccgtcgagcg ggacgggcgt cacctttctg gaagtctgac gccacccgga
     9781 cggcgggcct cgcgtcgcgg ggcccgcccc tcgttcccca gcaatcgagc cgagtgcccc
     9841 cgtccccgtg cccgcggagg cggggatcat tcctcatggg gcagccgctg cccgggtagg
     9901 aatggtcatc attcctgtcg gggcaatcgt tgccccacga ggaatgatgt gcgttcctgt
     9961 cggggcgatc gctgccccat gaggaatgga ggtcattcct gtcggggcag ccgctgcccc
    10021 atgaggaatg gagatcattc ctgtcggggc aacgcttgcc ccatgaggaa tggagggcat
    10081 tcctgccggg gcaacggctg ccccatgaga gacggccatt tcagccgcga agcggcgcag
    10141 caaaaccccc ctcctggcct cccccgctcc ctgccgcccg accccccagg gggggaggca
    10201 ccaccgcccc ctcgtgaggc agtccgtggg ggtccgtgat cgtccgtgtt ggtccgtgct
    10261 cgtcc
//
LOCUS       DASPJI010000110        18677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_408734, whole genome shotgun sequence.
ACCESSION   DASPJI010000110 DASPJI010000000
VERSION     DASPJI010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18677)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18677
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408734"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VEL74_02905"
     CDS             complement(<1..208)
                     /locus_tag="VEL74_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61505.1"
                     /translation="MRSLSHAAIRRPVQTLAAVLLAVLAAAPGLARLELRTDGLALAP
                     ARDPAVVYDRQVREEFGVRDSVAVV"
     gene            complement(285..986)
                     /locus_tag="VEL74_02910"
     CDS             complement(285..986)
                     /locus_tag="VEL74_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61506.1"
                     /translation="MKTWKTRSILAVIAVAACLAVPAQAAVSGPEAPVSNSFIAPDIG
                     GTAALPAIGSVYVSPADIHAWLRAGHELTAEMRASHGLFGNIITGPGGILQGEIETFD
                     SDLIFDITGVGPLKGWSTQVVIPTKVETHTGPRKLGEPVQSFETNMMRVEGQIKGHKD
                     FEYLEVVGGTANGLESPGQTTLYRQKDGNWLVESSFNVKFQLRYKGARGSKLEGLEGS
                     SEGVITMTAVPAPRG"
     gene            complement(1229..2317)
                     /locus_tag="VEL74_02915"
     CDS             complement(1229..2317)
                     /locus_tag="VEL74_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HYG61507.1"
                     /translation="MTRPQGEDLDVRLGGAPHPSGQTRARLLAVIATVLVLAFLKWSQ
                     VVTMPLAFAVFVIAVMWPLQERLQRHLPRWASLVLTVLVLLLVVALFVGALYWSGSLV
                     AERAPLYEPKLRALYGQLEGWARSHGISLGSQGQGGGSGQVAGDLAKRAGGAAAGSVS
                     LILLILALAILGLTEVSSFKRKTEHGFRHPERGPRIIESSRAIAARFQRYLVARTIAA
                     ILQGITVGLFALVIGLDFAFVWGVLAFLLNYIPSIGSVVAVIPPSLFALVQFDGLGRP
                     LAVLAGMAILQIVMGNYLDPLIEGKYLSISPVVVLISIVFWGWIWGVGGALLGVPLTV
                     GIMITSDQFPQTRWIARLLAEAPNRDEG"
     gene            2432..3223
                     /locus_tag="VEL74_02920"
     CDS             2432..3223
                     /locus_tag="VEL74_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYG61508.1"
                     /translation="MSIRKVLPAIVLCLLGGGCYRPQPTSVPLRTLPVASALPEGGER
                     CLVVLLPGRGDKPEDFVTNDFAVTLRQAGSTCEVLAVDAHLGYYYEGIIDTRLREDVI
                     VPALARGIEEVWLVGISLGGLGSILYTRDHPGEVAGVVLLAPFLGPDEVVNEVEEAGG
                     LSRWTPTEPLAERDLRRLWLWLKRYDAAAPRQPPIYLAYGQGDRFARKNALMAEALPE
                     DHVFVLPGGHTWRTWRRLWSAVLDAGVVPGKTAGRLRRATTGSSR"
     gene            complement(3204..5714)
                     /locus_tag="VEL74_02925"
     CDS             complement(3204..5714)
                     /locus_tag="VEL74_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HYG61509.1"
                     /translation="MRSLSFPCRCLSVLTLSLPALLGAHPAAAAPLMAYDDQLRNGFS
                     DWSWATRSMAQTAVVRTGTAAISFEPDGWAGLFFHRDLGIDTGAYEAVELWVHGSAPG
                     GQNVRIALTSGGDPVGNDAPLAGFVTGGAIPAGQWARALVPFASLGVTSGSFDGFWLQ
                     DGTGGDQPAIYVDDVQLIERSTPPPPPAAITVSMDLDADRRPISPLIYGVNFGSDAQA
                     ADLQWPVRRWGGNAVTRYSWQHDISNKASDWFYYNIESDTPNPATLPHGSTADLFIDA
                     TRNTGGEPIITVPLIGWTPIDRTRRWGFSVGKYGAQQKTECTETGNVFWCNPDAGNGV
                     YPNGTPITANDPHDTSREIGTGFVTDWMAHIASRTGAAGDGGVRFFALDNEPMLWNST
                     HRDVHPAPVTYDEMWQRTRDYAAAMKAEDPAIQILGPSDWGWCAYFFSAADGCSAGPD
                     AAAHGNVPFLPWYLQQVRAYELAHGVRLVDYLDIHYYPQGTNIALSSDESATTAARRL
                     RSLKGLYDPSYIDESWIGQPVRLIPRMKEWVASYLPGAKLAITEYNWGNDGGVTSALA
                     QAEALAIFGREGVDLATRWVAPAADTPVEDAFRLYLDYDGAGGKVEGDSVRAVSSNVD
                     AVGAYAVRGAGTRVYLLLFNKDTASHQTDIRVAGGLSGAVTLYRFGAAGRLAAAGTAT
                     LAGGALGLVLPSRSATLAVVELTEPPAGAGFYTLAPCRVLDTRQAAGAFGGPVLSAGA
                     GRQFPFAGRCGIPAGARAVSVNVTVVSPTQGGFVRAYPGGVTAPSTSLINFSAGQIRA
                     NNAILPLAADGNGTLVFQAGFTLPGTVHLLVDVNGYFQ"
     gene            5901..6968
                     /gene="ada"
                     /locus_tag="VEL74_02930"
     CDS             5901..6968
                     /gene="ada"
                     /locus_tag="VEL74_02930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HYG61510.1"
                     /translation="METNDTAWRAVLERDAAADGRFVYAVRSTGVYCRPSCPSRRPRR
                     EGVSFFSTPDEAERSGFRACRRCRPRASGPVGPARAVERACAWIEEHLDEPVTLEALA
                     SEVGLSPWHLQRTFKRLTGVSPSEYARARRLERLKTRLQEGDSVTDATYEAGFGSGSR
                     VYERSDAHLGMTPATYRKGGRGMRIRYSVASSPLGRLLVGMTGRGVCAVTLGDDEAAL
                     EASLRREYPQAEIERADPGDGELGERIAAVVRHLEGAEPLLTLPLDVQATAFQWRVWK
                     ALQEIPYGETRSYGEVAAALGQPRAARAVARACASNRVALVIPCHRVVPAEGGTGGYR
                     WGEERKRRLLERESRRSGETV"
     gene            6965..7873
                     /locus_tag="VEL74_02935"
     CDS             6965..7873
                     /locus_tag="VEL74_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HYG61511.1"
                     /translation="MSRSVPLTLELPPGFDLEWVFGFLAARVVPSLESLEDGVYRRSL
                     RLEDGPLVLTAERLHHPGAGALRVRSDPPRPRPRVREIVTRMLDLDADLAAFHALAAA
                     DPILRDLVPRCPGLRLPQYLDSFEGTMRAILGQQVSLAAASTMTDRLVRRFGEVLPAS
                     EGGAGLFTFPRPEVLAAAGPASLAAIGLTRAKSSALHNLAVAVADGTIDWEHLRHAPA
                     AEAEAALCALPGIGPWTASYVRMRTLGDRDAFPAADLGVLKAMERLGPGGPGGKRPAP
                     REVLARAESWRPWRAYATLHLWTSLS"
     gene            7987..8532
                     /locus_tag="VEL74_02940"
     CDS             7987..8532
                     /locus_tag="VEL74_02940"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HYG61512.1"
                     /translation="MKRFGLALLVVVLVATATPLFAQGNPGGTAIARLRGFEEVPAIS
                     TTGGGRFNATVNAQGTMVNWELNYFNLEAPVTQAHIHFAQRGVNGGVVVFLCSNLDNA
                     PVGVPGCPPSPGFLSGTFDAADVVAGAAAQGITPGELHSVLRAMRAGMTYVNVHTTLF
                     PGGEIRGQLLFNQGQGQGQGE"
     gene            complement(8581..9648)
                     /locus_tag="VEL74_02945"
     CDS             complement(8581..9648)
                     /locus_tag="VEL74_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYG61513.1"
                     /translation="MKLVSALLTLTLLAPYPAVSRQHAANRPPAAASRPAPRQEGPAR
                     PAPAAPGYKRAPGAHQVETSLFEWTDTARGRTVPVKIYSPQGAGPFPVIVFSHGLGGS
                     RDGYEYLGRHWASHGYVVVHVQHPGSDSSVWQGTLHPLKSMRAASSDPANSIARPLDV
                     RFTIDRLEALNREASPYKGRLDLEDLGVAGHSFGAYTALAVAGQMFVRGNREMSLADP
                     RVKAVLAMSAPAPARKWQADKSYSRIKVPVLHMTGTRDDSPIGDTRASERRIPFDRIS
                     AVDQYLLVLNGGDHMVFSGARRPTASSDKDPVFLDLILMSSTAFWDVYLRGDTAARAW
                     LANGGFEKALGAEGKLEKKLK"
     gene            complement(9835..11106)
                     /locus_tag="VEL74_02950"
     CDS             complement(9835..11106)
                     /locus_tag="VEL74_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017302189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HYG61514.1"
                     /translation="MQTAESRTGERVLRLEDEFVRSQFPALTGEWVFLDNAGGSQVLR
                     SAADRIRDFLLTSSVSLGAPYEASRLAGERVAEGALAGAALVNADPGEIVLGPSATQL
                     VVNLARAMTESFLHGDEIIVTNLDHEANISPWLRVATERGVIVKTWKVRPEAFELDLD
                     DLDALLTDRTKLVCIAQVSNILGAISPIGEVVQRAHARGARVFVDGVAYAPHRLVDVR
                     AWDVDFYVLSLYKVFGPRLGLLYGKREHLLELSNINHVYVPKDAIPYKLQPGGFSYEL
                     AAGVPAIVEYLEELGRRALDGETGEPAPRARLDAAFSAIAAAEAHAMAPLLAFLGERR
                     DVRLFGWPTASPAHRVSTVSVAVEGRDMADLARRLAERRIGVRAGDFHSRRLLQDLGV
                     PGGSVLRISLAHYNSPEEIEHLIDQLGELFR"
     gene            11639..12085
                     /locus_tag="VEL74_02955"
     CDS             11639..12085
                     /locus_tag="VEL74_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61515.1"
                     /translation="MNVLRALAGGFVGAGALTLIHESARRKIPNAPRADVLGMRAIDK
                     VMRKGDFLPPPDDRLHRWALAGDLVGNSLYYGLVGIGSPRGAWLRGGLLGLGAGLGAV
                     ALPGPLGLGKGPSGRTNATKAMTIAWYLAGGLVAAAVASWLGRRQG"
     gene            complement(12076..13692)
                     /locus_tag="VEL74_02960"
     CDS             complement(12076..13692)
                     /locus_tag="VEL74_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYG61516.1"
                     /translation="MTPAPDLPAPATERLRESEEQLRLALEASDTGVWTWDLGTDAVT
                     WSPECYRIHGLTEEEFDGTGAGFFQCVHPDDRERVERTVRAASESRGRYDCEFRILRH
                     GGGVLWVANRGRATYGAAGRPLRMVGTITDVTQRKRAEEELQRARSLLQAVIDGSTAL
                     VFAKDLEGRYFLTNQGWLDFVGLSAEQAAGITDEEAFGPEVAARVQANDRRVLETGEQ
                     LAFEESAMVRGRSVTYLSSKFPLTGPDGRVYAICGVSADITELKRTQAALAAREQELQ
                     TLTNALSEQDRRKDEFLATLSHELRNPLAPLRNGLAVLRMGCSQDQAAKVLGMMDRQL
                     GLMVHLVDDLLDLSRVSRGKITLRPERLTVREVVDAAVEACRPAIHAKEHALEVNLPA
                     EPLEVQGDRTRLVQVLSNLLTNAAKYTDAGGRLYLTAAREGGQAVLRVADNGVGIAPE
                     LLPTIWDMFSQVRETLDKAQGGLGIGLSLVRKLVEMHGGTVAAESPGLGRGSTFTVRL
                     QLAEAVHPRTEVPPAALGSDPALRLAPVQP"
     gene            14024..15781
                     /locus_tag="VEL74_02965"
     CDS             14024..15781
                     /locus_tag="VEL74_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018567228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HYG61517.1"
                     /translation="MNIIGISAGYHESSCCLLQEGRLVAAASEERFTRLKHDPGVPLH
                     AFRFCLERGGIGLTDIDAVAYYEIPAKKLARQLWAGRVSPGLPWLDALRAERTIRESL
                     GWEGPILSFEHHHSHAASAFFFSGYEEAAILTVDGVGEWATTTYGRGAGDSLDIFEEV
                     RFPHSLGLLYSALTSYLGFRVNGGEYKVMGLAPYGEPRHVDRLRRLVQPGPRGQYTLD
                     LQYFDFIRGERMYSPALCDLLGAPPRVPESPMEQFHQDVARSLQVLLEEILLEKVRYL
                     AGEVGSPNLCMAGGVALNCVANARILREGPFERLFVQPAAGDAGGSLGAAALAHRQLT
                     GRRPAAEPMRHAYLGPSYEAEEIARLIEAAGIQALDFRGREGDLLQASVDRLAAGRVL
                     GWFQGAMEFGPRALGSRSLLADPRDPQMRERLNRQVKKREAFRPFAPIVREERAAAHL
                     DLDHPSPFMLETCQVRSPLALPAITHVDGSARPQTVDREVAPRLAALLDAFEARTGCP
                     ILLNTSFNVRGEPVVCSPVDALICMAVSGIDTLVLEDWIIDRESLPGHWPELVDSVEK
                     VRGGVQPRASISEMVYTFI"
     gene            15794..17815
                     /locus_tag="VEL74_02970"
     CDS             15794..17815
                     /locus_tag="VEL74_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61518.1"
                     /translation="MSTGGGHEDGGGLPGMWDELAALLTGEQGLKTARLLQAAGLLGR
                     TPETPEPADRASATPPPRQAGGGQGRRICLIGESAAAGFFYAPFFTPALALGQFLAPF
                     GTFEVIDLARSGMSLDEVVETTAEALLEGPDLLVVFAGNNWLGLGPRPDQYADESLDS
                     FQEYADAVESRGTGGLRLLADERMRQRARSAVDRLGRLIAGTGIPLVFVVPAANLADF
                     ENLHPVPWLPGDGVARWYRLQGDVLAALEQGDTAAATSGARAMMELDGGHCATSHRLL
                     AAALLEAGETAEAHRALAAHLDTSCWDERLGRGSSAPAPVREALREGCGEHGFACVDL
                     TSALAELTGRPISDRRLFLDHCHHTAEGIRLAMAAVAAEVLRRFGTPGAPDARKLAAA
                     PVPGLPPVLVALAKLHAALYNAHMNRPVAGRHAPLTQRLLDEALDAAPGVREAILDYM
                     AAMSAPGRAFLTAACAHNGASQFPLQHLAWLPSDLGIDALEPMAMALRRRGVLSTPAA
                     EELARLLLQHHPPGRRVDISAPRYRERDPGATDTYALRQQPPTIFKALWPTSTFYLVA
                     DGERDLLLELTVRLPAMAGSSGVVAVEIDGERVGEVPASATWSRALLPVDRGALNRGL
                     NRVSLTWPLPGAVGDQALATAVERLRGGLPADIYPVFGEVFSLYAGPRT"
     gene            17925..>18677
                     /locus_tag="VEL74_02975"
     CDS             17925..>18677
                     /locus_tag="VEL74_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYG61519.1"
                     /translation="MEFQGTDKYYLDPELGAIVNMALAMEKPLLLTGEAGTGKTQLAF
                     EISRSLGMPIEVLRCKSTIKGEEACYVQDTVLRLNDARFGTAETGRDVNQFFDYVIWG
                     PIGRAFRSPRRVVLLLDEIDKTESDVQDNLLDVLEDSQFTIREVNQIIQADIKPFIVL
                     TSNAKRELSDPFLRRCFCHHIAFPDPAQMREIVKLHFPDIDKGLLDSALGIFYDLRQR
                     GYEKPPATSELLNWLGALQHARVTPDRLNIPFV"
BASE COUNT         2638 a         6483 c         6804 g         2752 t
ORIGIN      
        1 cgaccaccgc caccgagtcg cgcaccccga actcctcgcg aacctgccgg tcgtagacca
       61 cggcggggtc ccgggccggc gccagcgcga ggccgtcggt gcgcagctcg agccgcgcga
      121 ggccgggagc ggcggcgagg actgccagga gaacagctgc tagcgtctgg acgggacgcc
      181 ggatcgcggc atgggacagg gaacgcatgg acgcgggcac cggaaaagag aggggccccg
      241 gaccgccggc aaggcgatcc agggcccgat agccatccgt ggaatcagcc cctcggggcc
      301 gggaccgcgg tcatcgtgat aacgccctcg gagctgccct cgaggccctc gagcttgctg
      361 ccccgggcac ccttgtacct gagctggaac ttgacgttga agctgctctc caccagccag
      421 ttgccgtcct tctggcgata gagggtagtc tggccggggc tctccaagcc attggccgtg
      481 ccgcccacca cctcgaggta ctcgaagtcc ttgtggccct tgatctgacc ctcgacgcgc
      541 atcatgttgg tctcgaacga ctgcacgggc tcaccgagct tgcgcggacc ggtgtgggtc
      601 tcgaccttgg tcgggatcac cacttgggtg ctccaccctt tcagcggacc gacgccggtg
      661 atgtcgaaga tcaggtcgga gtcgaaggtc tcgatctcgc cttgcaggat gccgcccggc
      721 ccggtgatga tgttgccgaa gaggccatgg ctggccctca tctcggccgt gagctcgtgg
      781 ccggccctga gccaggcgtg gatatcggcg gggctgacat acacgctgcc gatcgcgggc
      841 agggcggcgg tgcctccaat atccggagcg atgaaggagt tggagacagg cgcttcggga
      901 ccgctgacgg cggcctgggc cgggaccgcc aggcaggcgg cgacggcgat gacggcgagg
      961 atgctacggg ttttccaggt cttcatgaaa caaaccccca cgtttcggtg tctccccccg
     1021 gaggcacacg agtcgatact ctgaccgcga cacgacgtca cggctcacac tccgcgcagg
     1081 ccgctatcct gtggcaacgg acctgcgcga atccccctcc tcaccgcgcg gtatcgaggg
     1141 cttcaatgtc tgtggtgcat gagcgctgga aaagcgctcc atgtgttgcg aaatcgacag
     1201 gggcagagga ggcgcgccgt tgcggagact agccctcatc ccgattcggc gcctcggcga
     1261 gcaggcgggc gatccagcgg gtctggggga actggtcgct ggtgatcatg atccccacgg
     1321 tgagagggac gccgagcagg gcgccgccta caccccagat ccagccccag aacacgatcg
     1381 agatgaggac gaccaccggt gagatggaga ggtacttccc ctcgatgagc gggtccaggt
     1441 agttgcccat cacaatctgg aggatggcca tgccggcgag gaccgccagc ggtctgccca
     1501 gaccatcgaa ctggaccagg gcgaagagcg acggcgggat gaccgccacc accgagccga
     1561 tgctggggat gtagttgagg aggaacgcga ggacgcccca gacgaacgcg aagtccaggc
     1621 cgatcaccag ggcgaagagg ccgacggtga tcccctggag gatggcggcg atggtgcgcg
     1681 ccaccagata gcgctggaac cgtgcggcga tggcgcggct ggactcgatg atccgggggc
     1741 cgcgctcggg gtgccggaac ccgtgctccg tcttgcgttt gaacgagctc acctcggtca
     1801 ggcccaggat cgcgagcgcc aggatcaaaa ggatgaggct gaccgacccg gcggcggccc
     1861 cgccggcgcg cttggccaga tcgccggcga cttggccgga gccgcccccc tgtccctgcg
     1921 atcccaggga gatcccgtgg gagcgcgccc acccctcgag ctgcccgtac agcgcccgca
     1981 gcttcggctc gtagagtggg gccctctcgg ccaccagcga gccgctccag tagagcgcgc
     2041 ccacgaagag ggcgacgacc agcaggagga cgaggacggt caggacgagg ctcgcccagc
     2101 gcggcaggtg gcgctggagc ctttcctgca gaggccacat caccgcgatg acgaagacgg
     2161 cgaaggccag gggcatggtc accacctggc tccacttgag gaaggccagg accaggacgg
     2221 tagcgatgac cgccagcagg cgcgcccggg tctgcccgga gggatggggc gcgcccccga
     2281 gacggacgtc gaggtcctct ccctgtggtc ttgtcacagc ctgctctctg ggcacagact
     2341 tcctgtccat aacgccggga ggaagactgc aacgctcgtg ccgcggggca ggcgcggagc
     2401 ctgcattcga gggagaggag ggggggattc gatgtcgatc cgaaaagtac tgccggctat
     2461 tgtcctgtgt cttctggggg ggggatgcta ccggccccag cccacctcgg ttccgctacg
     2521 gacgctgccg gtcgcttccg cgcttccaga gggcggcgag cgttgcctgg tagttctcct
     2581 gcccggccgg ggggacaagc ccgaggactt cgtcaccaac gacttcgccg tcaccctgcg
     2641 gcaggcgggc tccacctgcg aggttctggc cgtggatgcc cacctgggct actactacga
     2701 gggcatcatc gacacccggc tgcgggagga cgtcatcgtt ccggccctcg cccgaggcat
     2761 cgaggaggtc tggctggtcg gcatctccct gggcgggctc ggctcgattc tctatacccg
     2821 ggatcatccc ggcgaggtgg caggcgtcgt gctgctcgcg ccgttcctgg gtccggacga
     2881 ggtggtgaac gaggtggagg aggcgggcgg cctgagccgc tggactccca cggagccgct
     2941 ggcggagcgc gacctccggc gcctctggct ctggctcaag agatacgacg ccgccgctcc
     3001 ccgccagccc cccatctatc tcgcctacgg ccagggggac cgcttcgccc gcaagaacgc
     3061 cctgatggcc gaggcgctgc cggaggacca cgtcttcgtc ctgcctggcg ggcacacctg
     3121 gcgcacctgg cggcgcctct ggtccgccgt cctcgacgcc ggcgtcgtgc cggggaagac
     3181 ggcgggccgg cttcggcggg ctactactgg aagtagccgt tgacgtccac cagcaggtgg
     3241 acggtccccg gcagggtgaa gcccgcctgg aagacgagcg tgccgttgcc gtccgccgcc
     3301 aggggcagga tggcgttgtt ggcccggatc tgaccggccg agaagttgat caggctggtg
     3361 gacggcgccg tgacgccccc cggataggcg cggacgaagc cgccctgagt gggggagacc
     3421 acggtgacgt tgaccgaaac cgcccgcgcc cccgccggga tgccgcagcg gccggcgaag
     3481 gggaactggc ggccggcgcc ggcggagagc accgggccgc caaaggctcc ggctgcctga
     3541 cgggtgtcca gcacccggca gggggcgagg gtgtagaagc ccgcgccggc gggcggctcc
     3601 gtcagctcca ccaccgccag ggtcgccgag cgggatggca ggacgagccc gagggcgccg
     3661 ccggcgaggg tggcggtacc ggccgcggcg agccgccccg cggcgccgaa ccggtagagg
     3721 gtcacggctc ccgagaggcc gccggccacc cggatatcgg tctggtggct ggcggtgtcc
     3781 ttgttgaaga ggaggagata gacgcgcgtg ccggcgccgc ggacggcata ggcgccgacg
     3841 gcgtccacat tggagctgac cgcccgcacg ctgtccccct ccaccttccc ccccgcgccg
     3901 tcgtagtcca gatagagcct gaaggcgtcc tccaccggag tgtccgccgc cggggcaacc
     3961 cagcgggtgg cgaggtccac cccttcgcgg ccgaagatgg ccagcgcctc ggcctgggcc
     4021 agggcgctgg tcacgccgcc gtcattgccc cagttgtact cggtgatggc cagcttggcg
     4081 cccgggaggt agctggcgac ccactccttc atccgcggga tcaggcggac cggctggccg
     4141 atccacgact cgtcgatgta ggaaggatcg tagagcccct tgagggagcg cagccgccgc
     4201 gccgccgtcg tggccgactc gtcgctggag agagcgatgt tggtgccctg cgggtagtaa
     4261 tggatgtcca ggtagtccac cagccgcacc ccgtgggcga gctcgtaggc ccgcacctgc
     4321 tggaggtacc aggggaggaa cgggacgttg ccgtgggccg cggcgtcagg gccggccgag
     4381 cagccgtccg ccgccgagaa gaagtaggcg caccaccccc agtccgacgg gccgaggatc
     4441 tggatcgccg gatcctcggc cttcatcgcc gcggcatagt cccgggtccg ctgccacatc
     4501 tcgtcatagg tcaccggcgc cgggtggacg tcccggtggg tgctgttcca gagcatgggc
     4561 tcgttgtcca gggcgaagaa gcggacgccg ccgtctccgg cagcgccggt gcgggaggcg
     4621 atgtgggcca tccagtcggt gacgaagccc gtgccgatct cgcgcgaggt gtcgtgcggg
     4681 tcgttggcgg tgatcggggt gccgttcggg tagacgccgt tgcccgcgtc ggggttgcac
     4741 cagaagacgt tcccggtctc ggtgcactcg gtcttctgtt gcgcgccgta cttgccgacc
     4801 gagaagcccc agcggcgggt gcggtcgatg ggcgtccagc cgatgagcgg cacggtgatg
     4861 atcggctcgc cgccggtgtt gcgggtggcg tcgatgaaca ggtcggcggt ggagccgtgg
     4921 ggcagggtgg ccgggttcgg ggtgtcgctc tcgatgttgt agtagaacca gtcgctcgcc
     4981 ttgttcgaga tgtcgtgctg ccaggagtag cgggtgacgg cgttgccgcc ccagcgccgc
     5041 accggccact ggaggtccgc ggcctgggcg tccgagccga agttgacgcc atagatcagg
     5101 gggctgatcg gccggcggtc ggcgtcgagg tccatgctga cggtgatcgc cgcggggggc
     5161 ggggggggtg tggaccgctc gatgagctgc acgtcatcga catagatcgc cggctggtcc
     5221 cccccggtgc cgtcctgcag ccagaagccg tcgaagctgc cggacgtgac ccccaggctg
     5281 gcgaagggga cgagcgcccg cgcccactgc ccggccggga tggcgccgcc ggtgacgaac
     5341 ccggccagcg gcgcgtcgtt gcccacgggg tcgccgcccg aggtcagggc aatccgcacg
     5401 ttctggccgc ccggggcgct gccgtggacc cacagctcga ccgcctcgta ggcgccggtg
     5461 tcgatcccca ggtcgcgatg gaagaagagc ccggcccagc cgtccggctc gaacgagatc
     5521 gccgccgtcc cggtgcggac cacggccgtc tgggccatgc tccgggtcgc ccagctccag
     5581 tcggagaacc cgttcctgag ttggtcgtcg taggccatga gcggcgccgc ggcggcggga
     5641 tgggctccga gcagcgccgg cagagagagg gtgaggacgg agagacagcg gcagggaaag
     5701 gaaagagatc tcatggtcgc ctcccgaaac ggattcaatg catgcttgtc tatgcgtcga
     5761 actgtagcac gcccacttcg accgcaagat ccgggtggtc ccggtcgagc cccgcgtcct
     5821 actcttccga aggatcgtta cggaagggag gatctatgac atcgtggaca acggctcttc
     5881 ggcaggatca ggatagagaa atggagacga acgacacggc ttggcgggcc gtcctggagc
     5941 gcgacgcggc ggcggatggg cggttcgtct atgccgtccg ctcgaccggc gtctattgcc
     6001 ggccgtcgtg cccgtcgcgc cggccgcggc gggagggggt gagcttcttc tccacccccg
     6061 acgaggcgga gcggtccggc ttccgcgcct gccggcgctg ccgcccacgg gccagcgggc
     6121 cggtggggcc ggcgcgggcg gtcgagcggg cctgcgcctg gatcgaggag cacctcgacg
     6181 agccggtgac tctcgaagcc ctcgcttcgg aagtggggct cagcccctgg cacctccagc
     6241 gcacattcaa gcggctcacc ggggtctccc ccagcgagta cgcccgcgcc cgccgcctcg
     6301 aacggctcaa gacccgcctc caggaggggg acagcgtcac cgacgccacc tacgaggccg
     6361 gcttcggctc cggcagccgc gtctacgagc ggtcggacgc gcacctgggg atgacgcccg
     6421 ccacctatcg caaggggggg cgggggatgc gcatccgcta ctccgtcgcc tcgtcccccc
     6481 tcggccgcct gctcgtgggg atgaccggcc gtggcgtctg cgccgtcacc ctgggcgacg
     6541 acgaggcggc gctcgaagcg tcccttcggc gcgagtaccc gcaggccgag atcgagcggg
     6601 ctgatcccgg ggatggggag ctcggagagc ggatcgccgc cgtggtccgg catctggaag
     6661 gggcggagcc gctccttacc ctgccgctcg acgtccaagc gaccgcgttc cagtggcggg
     6721 tgtggaaggc gctccaggag atcccctatg gcgagacccg gtcctacggc gaggtcgccg
     6781 cggccctcgg ccagccgcgg gcggcccggg ccgtggcgcg cgcctgcgcc agcaaccgcg
     6841 tcgccctggt catcccttgc caccgggtgg tgccggccga gggcggcacc ggcggctacc
     6901 gctgggggga ggagcgcaag cgccggctcc tcgagcggga gagccggcgg agcggggaga
     6961 ccgtgtgagc cggagcgtcc ccctgaccct ggagctgccg cccggcttcg accttgagtg
     7021 ggtcttcggc ttcctggccg cccgggtggt gccgtccctg gagagcctgg aggacggggt
     7081 ctaccggcgg agcctgcgcc tggaggacgg gccgctggtt ctcacggccg agcgcctgca
     7141 ccaccctggc gcgggcgccc tccgggtacg ctcggacccg ccgcgtcccc ggccccgggt
     7201 gcgcgagatc gtcacccgga tgctcgacct ggacgccgac ctcgcggcgt tccacgccct
     7261 ggcggcggcg gacccgatcc tgcgcgacct ggtcccccgc tgtccggggc tccgtctgcc
     7321 acagtacctc gactccttcg aggggacgat gcgggcgatc ctcggccagc aggtgagcct
     7381 cgccgcggcg agcaccatga ccgaccgtct ggtgcgccgc ttcggcgagg tgctgccggc
     7441 ctcggaaggc ggtgccggcc tgttcacctt tccccgcccc gaggtcctgg cggcggcggg
     7501 cccggcgtcc ctcgccgcga tcgggctcac ccgcgccaag tcgtcggcgc tccacaacct
     7561 cgccgtggcg gtggccgacg gcacgatcga ctgggagcat ctgcgccacg ccccggcggc
     7621 ggaggccgag gccgccctct gcgcgctgcc cggcatcggg ccgtggaccg cctcctacgt
     7681 ccgcatgcgc accctcggcg accgcgacgc cttcccggcg gccgacctgg gagtgctcaa
     7741 ggcgatggag cggctgggtc ccgggggccc gggcggcaag cggccggcgc cgcgcgaggt
     7801 cctcgcccgc gccgagtcct ggcggccgtg gcgggcctac gcgaccctcc atctctggac
     7861 ctcactctcc tgagccagtt ctctctataa tgatcccgta gctctgaagt ctggctgtac
     7921 cttctctgat cagggtagga ccgcgtggag gccatgaccg cgcccatcgg gcgcaggagg
     7981 gattccatga agaggttcgg tctggctctg ctcgtcgttg ttctcgtcgc caccgccaca
     8041 ccgctgttcg cccaggggaa tcccgggggg acggccatcg cccggctccg cgggttcgag
     8101 gaggtgccgg cgatctcgac caccggcggc ggccgcttca acgccacggt caacgcgcag
     8161 ggcacgatgg tcaactggga gctgaattac ttcaacctgg aagcgccggt cacccaggcc
     8221 cacatccact tcgctcagcg cggggtcaac ggcggcgtgg tggtcttcct ctgctcgaac
     8281 ctggacaatg ccccggtggg cgtgccgggg tgtccgccct cgccggggtt cctgtccggc
     8341 acgttcgacg cggcggacgt ggtcgccggc gcggcggcgc agggcatcac gcccggtgag
     8401 ctgcattcgg tcctgcgggc gatgcgcgcc ggcatgacct acgtgaacgt ccacaccacg
     8461 ctcttccccg gcggcgagat ccgcgggcag ctcctcttca atcaggggca agggcaggga
     8521 cagggggagt agtcgcgggt ggaccggatg gccggcgggt ggtcccgccg gcctcccccc
     8581 tcacttgagc ttcttctcga gcttcccttc ggccccgagg gccttctcga acccgccgtt
     8641 ggccagccac gcccgggccg cggtatcccc ccgcaggtag acgtcccaga aggcggtgga
     8701 gctcatgagg atgaggtcga ggaagaccgg gtccttgtcg ctggacgccg tcggcctgcg
     8761 cgctcccgag aagaccatgt ggtcgccgcc gttgagcacg aggagatact ggtcgacggc
     8821 cgagatgcgg tcgaagggga tgcggcgctc cgacgcgcgg gtgtcgccga tcgggctgtc
     8881 gtcgcgggtg ccggtcatgt ggagcaccgg caccttgatc cgggagtagg acttgtcggc
     8941 ctgccacttg cgggcgggag ccggcgcgct catggcgagg acggccttga cccggggatc
     9001 ggccagcgac atctcgcggt tcccacggac gaacatctgg cctgccacgg cgagcgccgt
     9061 ataggcgccg aaggagtggc cggcgacgcc caggtcctcg aggtccagcc gccccttgta
     9121 gggggaggcc tcgcggttca gcgcctccag ccggtcgatg gtgaagcgga cgtccagcgg
     9181 gcgggcgatg gagttggccg gatcggagga ggcggcccgc atggacttca acgggtgcag
     9241 ggtgccctgc cagaccgagc tgtcgctccc cgggtgctgg acgtgcacca ccacgtagcc
     9301 gtggctcgcc cagtggcggc ccaggtactc gtagccgtcc cgcgagccgc cgaggccgtg
     9361 cgagaagacg atgaccggga acggcccggc gccctggggg gagtagatct tgaccggtac
     9421 cgtccggccg cgcgccgtgt cggtccactc gaagagggag gtctcgacct gatgggcgcc
     9481 gggggcccgc ttgtagcccg gggcggccgg cgccgggcgg gccggccctt cctgccgggg
     9541 cgcggggcgc gaagccgccg cggggggccg gttggccgcg tgctggcggc tgaccgccgg
     9601 atacggcgcc agcagcgtca gagtcaggag tgccgatacg agcttcaaga cgcggtgccc
     9661 ctcagggagg ggagtatatc gtgcgggccc tcaccccctg accccctctc ccgcctcccc
     9721 tcccccctct ccgggagagg gggaagctgg tttgagcgtt ctgttggggt gtggtggcgc
     9781 ccctctcccg gggaggggga atggagtggc gggagagggg acgggggtga gggcctaccg
     9841 gaacagctcc ccgagctggt cgatgaggtg ctcgatctcc tccgggctgt tgtagtgggc
     9901 gagggagatg cggaggacgc tgccgccggg gactcccagg tcctggagca ggcgacggga
     9961 gtggaaatcg ccggcccgga cgccgatccg gcgctcggcg agccggcggg cgaggtcggc
    10021 catatcgcgc ccctcgaccg cgacgctgac cgtcgagacc cgatgagccg ggctcgcggt
    10081 gggccagccg aagaggcgga cgtcccggcg ctcaccgagg aaggccagga gcggcgccat
    10141 ggcgtgcgcc tcggcggcgg cgatggcgga gaaggcggcg tcgagccgag cgcggggcgc
    10201 cggctctccg gtctcaccgt ccagagcgcg gcggcccagc tcctccaggt actcgacgat
    10261 cgccggcacg ccggccgcca actcgtagct gaatcccccc ggctggagct tgtaggggat
    10321 cgcgtccttc ggcacgtaga cgtggttgat gttcgacagc tccagcaggt gctcccgctt
    10381 gccgtagagg agcccgaggc gggggccgaa caccttgtag aggctcagga catagaaatc
    10441 gacgtcccag gcgcgcacgt ccaccagccg gtgcggggca taggcgacgc cgtccacgaa
    10501 gacgcgggcc ccccgcgcgt gcgccctctg gaccacctct ccgatggggc tgatggcgcc
    10561 gaggatgttc gagacctggg cgatgcagac cagcttcgtg cggtcggtga gcagggcgtc
    10621 gaggtcgtcg aggtccagct cgaaagcctc cgggcggacc ttccaggtct tgacgatgac
    10681 gccgcgctcg gtggccaccc gcagccacgg gctgatgttg gcctcgtggt cgaggttggt
    10741 gacgatgatt tcgtcgccgt gcaggaagct ctcggtcatg gcgcgggcga ggttgaccac
    10801 gagctgggtg gccgacggcc cgagcacgat ctcccccgga tccgcgttga ccagcgccgc
    10861 cccggccagc gccccctccg ccacccgctc gcccgccagg cgcgacgcct cgtagggcgc
    10921 ccccagggag acgctggagg tcagcaggaa gtcgcggatc cggtcggccg cgcttcggag
    10981 cacctgggag cccccagcgt tgtcgaggaa cacccactca cccgtgaggg cggggaactg
    11041 cgagcggacg aattcgtcct ccagccggag gaccctctcc ccggtccggg attcggcggt
    11101 ctgcatgggc atggtctcca tgagcggggt cgaggcgaag tgtagcaggt cgggggaggc
    11161 gcttttctct tggcggagct tgcagcggcg cgccggaagc tccgccggcg gattggaagc
    11221 ttgcaacggc ggatcggaag cttgcaccgg cttggcgccg agcgccgcgg cgcgttggcg
    11281 gctgcgccgg cgcgttggca ggctgcaacg gcctggcgga gagtgcaacg gcctgacgga
    11341 gattgcaatg gcgtgttgcg gctctgcgcc ggcgtcgcgg agcttgcaac ggcccgggga
    11401 gccctgcaac aaggcgttgt gcccttcccg gcggagagga agcttgcaac ggcttggcga
    11461 aggctgcaac gaggcgttgg cggctgcgcc ggcgcgttgc aaggctgcga cggagcgttg
    11521 tcagcttcgc caaggcgttg caagcttgcg gcggcctgtt ggaggctgcg ccgcttcgcg
    11581 gctgacggag gcggggcgcg gagattgcag cgacggccag tgggaggagg aagacttcat
    11641 gaacgtgctg cgagccctgg ccggcggttt tgtgggagcg ggcgctctca ccctcatcca
    11701 tgaatcggcg cgccggaaga tcccgaacgc gccccgcgcc gacgtgctcg gtatgcgggc
    11761 catcgacaag gtcatgcgca agggggactt cctgccgccg ccggacgacc ggctccaccg
    11821 ctgggccctg gcgggcgatc tggtcggcaa ctcgctctac tacggccttg tcgggatagg
    11881 gagcccgcgc ggggcgtggc tccggggcgg tctcctcggt ctcggtgccg gcctcggcgc
    11941 cgtggccctg cccggaccgc tcggcctcgg caagggaccg agcgggcgga ccaacgccac
    12001 gaaggccatg accatcgcct ggtacctggc cggcgggctc gtcgccgcgg ccgtggcgag
    12061 ctggctggga cggcgtcagg gctgaacggg tgccagccgg agggccgggt cggagcccag
    12121 tgctgccggc ggcacctccg tcctcggatg gacagcctcg gcgagctgca gacggacggt
    12181 gaacgtgctc ccgcggccga ggccggggct ctcggccgcc accgtgccgc cgtgcatctc
    12241 caccagcttc ctgaccagcg acaggccgat ccccaggccc ccctgcgcct tgtccagcgt
    12301 ctcccggacc tggctgaaca tgtcccagat ggtggggagc agctcgggag cgatgccgac
    12361 gccgttgtcc gccacccgga ggacggcctg ccctccctcc cgcgccgcgg tgagataaag
    12421 gcggccaccc gcgtcggtgt acttggccgc gttggtcaac aggttcgaga gcacctggac
    12481 cagccgcgtt cggtccccct ggacctccag cggctcggcc gggaggttca cctccagcgc
    12541 gtgctccttc gcgtggatgg ccggccggca cgcctcgacg gccgcgtcca ccacctcccg
    12601 gaccgtcagc cgctccggcc gcagggtgat cttcccccgg ctcacacgcg acaggtcgag
    12661 caggtcgtcg accaggtgga ccatcagccc gagttgccgg tccatcatgc cgagcacctt
    12721 cgcggcctga tcctgagagc agcccatgcg caggacggcc agcccgttgc ggagcggggc
    12781 gagcgggttg cgcagctcat gggagagcgt ggccaggaac tcgtccttgc ggcggtcctg
    12841 ctccgatagg gcgttggtga gcgtctggag ctcctgctcg cgggcggcga gggcggcctg
    12901 agtccgcttg agctcggtga tgtcggccga gactccgcag atcgcgtaca cccggccgtc
    12961 cgggccggtc agcgggaact tgctcgacag ataggtcacg gaccggcccc gaaccatcgc
    13021 ggactcctcg aacgcgagct gctcgccggt ctccagcacg cggcggtcgt tggcctgcac
    13081 cctggccgcg acctcggggc cgaaggcctc ctcgtcggtg atcccggccg cctgctcggc
    13141 cgacagcccg acgaaatcca gccacccctg gttggtgagg aagtatcggc cctccaggtc
    13201 tttggcgaag accagggcgg tggagccgtc gatgaccgcc tggaggaggg accgtgcccg
    13261 ctgcagctcc tcctcggccc gcttgcgctg ggtaacgtcg gtgatggtgc cgaccatccg
    13321 cagcggccgg cccgcggcgc cgtacgtcgc gcggccccgg ttcgccaccc agagcacgcc
    13381 gcctccgtgc cggaggatgc ggaattcgca gtcatagcgg ccgcgggact cggaggctgc
    13441 ccggaccgtc ctctccaccc gctcccggtc gtcggggtgg acgcactgga agaacccggc
    13501 gccggtgccg tcgaactcct cttcggtcag cccgtggatg cggtagcatt cgggcgacca
    13561 ggtgacggcg tccgtgccga ggtcccacgt ccacacaccg gtgtcggacg cctccagcgc
    13621 caagcgcagc tgttcctcgc tctcacggag ccgctccgtc gccggagccg gcaggtctgg
    13681 ggccggcgtc aatcaacgct cctcgcatgc acggcagtag tttagtatca gttggtgatt
    13741 ccgtgctggc aagggtagat gatacgccgg ttggggaggg tcggtggtgg cggcgatggc
    13801 cggcgggagg gccgaggcgg gggcggcgag cgcgagcagc agggcggcgg cgatcgattt
    13861 cgtcattcag ggggctccgg agtcaggggg gcctgatgtt tatacgtttc aggagagggg
    13921 tgcgttgtgc cggctccggc gtcctcgcga tcctggcgtc tcgctctcgc ccctctgccg
    13981 cactagctct tgggatgtgc ttcagcaggg agcggtcagg gacatgaaca tcatcggcat
    14041 ctcggccgga tatcacgaat cgtcatgctg cctgctgcag gaggggcggc tggtggcggc
    14101 ggcgtcggag gagcggttca cgcggctcaa gcacgacccg ggggtgccgc tccatgcctt
    14161 ccggttctgc ctggaacgtg gcggcatcgg cctgaccgac atcgacgcgg tcgcctacta
    14221 cgaaatcccg gcgaagaagc tggcacggca gctctgggcc ggacgtgtca gcccggggct
    14281 gccctggctg gacgccttgc gcgccgagcg gacgatccgg gagagcctgg gatgggaggg
    14341 gcccatcctc tcgttcgagc accaccactc gcatgccgcg agcgccttct tcttctcggg
    14401 atacgaggag gcggccatcc tcacggtgga cggggtgggc gagtgggcca ccaccaccta
    14461 cggccggggc gccggcgact ccctcgacat cttcgaggag gtgcgcttcc cgcactcgct
    14521 cggcctgctc tacagcgccc tgaccagcta tctgggtttc cgggtcaacg gcggcgagta
    14581 caaggtcatg gggctcgcac cctacggcga gccgcgccat gtggaccggc tgcgccggct
    14641 cgtccagccg gggccgcgcg gacagtacac cctcgacctc caatatttcg atttcatccg
    14701 cggcgagcgg atgtactccc cggccctctg cgacctcctg ggggcgccgc cacgggtgcc
    14761 cgagtcgccg atggagcagt tccaccagga cgtcgcgcgc agccttcagg tgctgctcga
    14821 ggagatcctg ctggagaagg tccgctacct ggcgggggag gtcgggtcgc ccaacctctg
    14881 catggcgggg ggagtggcgc tcaactgcgt ggccaacgcc cggatcctgc gggaagggcc
    14941 gttcgagcgc ctgttcgtcc agccggcggc cggcgacgcg gggggctccc tgggcgccgc
    15001 ggcgctcgcc caccgccagc tcaccggccg gcgcccggcg gcggagccga tgcgccacgc
    15061 ctatctgggg ccctcctacg aggcggagga gatcgcccgg ctgatcgagg cggccggcat
    15121 ccaggccctc gatttccgcg ggcgcgaagg ggacctcctg caggcgtcgg tggaccggct
    15181 cgccgccggc cgggtgctgg gctggttcca gggcgcgatg gagttcggcc cgcgggcgct
    15241 ggggtcgcgc agcctcctcg ccgatccgcg ggacccgcag atgcgcgagc gcctcaaccg
    15301 ccaggtcaag aagcgcgaag ccttccggcc gttcgccccc atcgtccgcg aggagcgggc
    15361 cgccgcccat ctcgacctgg accacccgtc gcccttcatg ctggagacct gccaggtgcg
    15421 ctcgcccctc gcgctgccgg cgatcaccca cgtcgacggc tcggcccggc cgcagacggt
    15481 cgaccgcgag gtggcgcccc gcctggcggc gctgctcgac gccttcgaag cacggacggg
    15541 ctgccccatc ctgctcaata cgtcgttcaa cgtccgcggc gagcccgtcg tctgctcgcc
    15601 ggtggacgcc ctgatctgca tggcggtctc ggggatcgat acgctggtcc tggaggactg
    15661 gatcatcgac cgggagagtc tccccggtca ctggcccgag ctggtggact ccgtcgagaa
    15721 ggtccggggc ggagtgcagc ccagggcgtc gatcagcgag atggtctaca ccttcatctg
    15781 agggagagag cggatgagca ccggcggtgg acacgaagac ggcgggggcc tcccggggat
    15841 gtgggacgag ctggcggcgc tgctcaccgg cgagcagggc ctgaagacgg cccgcctcct
    15901 ccaggccgcc ggcctgctgg ggaggacccc cgagacgccg gagccggccg atcgggcgag
    15961 cgccacgccg ccgccccggc aggcgggcgg cggccagggc cggcggatct gcctgatcgg
    16021 cgagtccgct gcggccggct tcttctatgc ccccttcttt acaccggccc tggccctcgg
    16081 tcagttcctc gcccccttcg gcaccttcga ggtgatcgac ctggcgcggt cgggcatgtc
    16141 gctcgacgag gtggtggaga ccacggccga ggccttgctg gaggggccgg acctgctggt
    16201 ggtgttcgcc ggcaacaact ggctcggcct gggaccgcgt ccggaccagt acgccgacga
    16261 gagcctcgac agcttccagg agtacgcgga cgcggtggag agccggggca ccggcggcct
    16321 gcggctgctg gcggacgaac ggatgcggca acgggcgcgc tccgccgtcg accgtctggg
    16381 acggctcatc gccggcaccg gcatccccct ggtgttcgtc gtcccggcgg cgaacctggc
    16441 cgacttcgag aacctccacc cggtgccctg gctccccggc gatggcgtcg cgcgctggta
    16501 ccgtctccag ggcgacgtgc tggcggcgct ggagcagggg gatacggcgg cggccacctc
    16561 tggggcgcgg gcgatgatgg agctcgacgg cggccactgc gccacgtcgc accgtctcct
    16621 cgccgcggcc ctgctggaag cgggagagac cgccgaggcc catcgcgcgc tcgccgccca
    16681 cctggacacc tcctgctggg acgagaggct cggccggggc tcctcggctc cggcgccggt
    16741 gcgggaggca ctgcgcgagg ggtgcggcga acacggcttc gcctgcgtcg atctgacgtc
    16801 cgccctcgcc gagctcaccg gccgtcccat ctccgaccgg cggctgttcc tcgaccactg
    16861 ccatcacacc gccgaaggga tccggctggc catggccgcg gtggccgcgg aggtgctgcg
    16921 ccgcttcggg acgccgggag ctcccgacgc ccggaagctg gcggcggcac cggtgcccgg
    16981 cctgccgccg gtcctcgtcg ctctggcgaa gctccacgcc gccctctaca acgcccacat
    17041 gaaccgtccg gtggccggcc ggcacgcgcc gctgacccag aggctcctgg acgaggcgct
    17101 ggacgccgcc ccgggagttc gggaggccat cctcgactac atggcggcga tgagcgcacc
    17161 gggccgcgcc ttcctgaccg cggcctgcgc ccataacggc gcctcccagt tccccctcca
    17221 gcacctggct tggctccctt cggacctggg catcgacgcc ctggagccca tggccatggc
    17281 cctgcggcgg cggggtgtcc tgtccacgcc ggcggcggag gagctcgccc gcctgctcct
    17341 ccagcaccac ccgcccggcc ggcgggtcga catctcggcg ccgcgctacc gcgagcgcga
    17401 tcccggagcc acggacacct atgccctgcg ccagcagccg cccacgatct tcaaggcgct
    17461 gtggcccacc tcgaccttct atctggtggc cgacggcgag cgggacctcc tcctggagct
    17521 gactgtgcgc ctgcccgcca tggcggggtc ctcgggggtg gtggccgtcg agatcgacgg
    17581 cgagagggtc ggagaggtcc cggcctcggc gacgtggagc cgtgccctgc tgccggtcga
    17641 ccgcggggcc ctgaaccgcg gcctgaaccg ggtgagcctg acctggcccc tgccgggagc
    17701 cgtcggtgac caggccctcg ccaccgccgt cgagcgcctg cgcggggggc tccccgccga
    17761 tatctacccc gtcttcggag aggtgttctc cctctacgcc ggtccccgga cctgaggcct
    17821 ccccccctcg gaggcgtctg ctagactctg ccccctgcgc ttcgcggcat cgcctctcgg
    17881 catcgccccc gcgaggcgct cttcccccgc aggagatccg agagatggag tttcaaggta
    17941 ccgacaagta ctacctcgac cccgagctgg gcgccatcgt caacatggcg ctggcgatgg
    18001 agaagccgct gctcctcacc ggcgaggcgg gcacgggcaa gacccagctc gcgttcgaga
    18061 tcagccgctc cctgggcatg cccatcgagg tcctgcgctg caagtccacg atcaaggggg
    18121 aggaggcctg ctacgtccag gacacggtgc tgcgcctgaa cgacgcccgc ttcggcaccg
    18181 ccgagaccgg ccgcgacgtc aaccagttct tcgactacgt gatctggggg ccgatcggcc
    18241 gcgccttccg cagcccccgg cgcgtcgtcc tcctgctcga cgagatcgac aagaccgagt
    18301 ccgatgtcca ggacaacctg ctcgacgtgc tggaggactc ccagttcacc atccgcgagg
    18361 tgaaccagat catccaggcg gacatcaagc cgttcatcgt cctgacttcg aacgccaagc
    18421 gcgagctgtc cgaccccttc ctgcggcgct gcttctgcca tcacatcgcc ttccccgacc
    18481 cggcgcagat gcgggagatc gtcaagctcc acttcccgga catcgacaag gggctgctcg
    18541 actcggcgct cggcatcttc tacgacctgc gccagcgcgg ctacgagaag ccgccggcga
    18601 ccagcgagct gctcaactgg ctcggcgcgc tccagcacgc ccgggtgacg ccggaccggc
    18661 tgaacatccc gttcgtc
//
LOCUS       DASPJI010000111         3066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_296448, whole genome shotgun sequence.
ACCESSION   DASPJI010000111 DASPJI010000000
VERSION     DASPJI010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296448"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VEL74_02980"
     CDS             complement(<1..387)
                     /locus_tag="VEL74_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HYG61520.1"
                     /translation="MIDAKDREILTILQGNARISNAEIARQVGMAPSAILERIRRMEA
                     SGVIQGYETRVSPEALGYHLLAFVFVATNDFAGELKTAERLSAIPEVQEVHHIAGEDC
                     FLVKIRARDAKGLGRLLREQLGQMRTR"
     gene            complement(534..1607)
                     /gene="tsaD"
                     /locus_tag="VEL74_02985"
     CDS             complement(534..1607)
                     /gene="tsaD"
                     /locus_tag="VEL74_02985"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HYG61521.1"
                     /translation="MSAPPLVVPLVLGIETSCDDTACAVLDGDGRVLASVVSSQLAAH
                     RPYGGVVPEIASREHLANWPLVSREALERAGLPLADIDVVAATRGPGLVGALLVGLSL
                     GKAIAFGLERPFHAVHHLEGHLYSPFLDAAGGPAGPPPARFTGLVLSGGHSSLFAVGE
                     GEDGAVSTLAETRDDAMGEVFDKVGKRLGLPYPQGPKVDELAERGDASRHRFPTPSCG
                     DSLDFSYSGLKSQALQAIERIEAVSGPVDLSRQEPSASILDLVAGFRAATVAQVLDRL
                     ERLHRERPLELLAVSGGVAANRLLRRELPAWAEARGIPLRLVPMAFAGDNAAMIAHAA
                     LLRHRRGLADDPFAAEAESRIPM"
     gene            complement(1701..2054)
                     /locus_tag="VEL74_02990"
     CDS             complement(1701..2054)
                     /locus_tag="VEL74_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012409806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61522.1"
                     /translation="MAEMGKYCKAYLAKQFREYPSWTENAANVRKEKKEVNGKEVEVE
                     RELNDDSILYLQENYVVTDGIFKDENIIFDNVTDEWKDYCHNTLAFEIPVYEPINIPK
                     AEDAGSGDAPAGGGA"
     gene            2331..>3066
                     /locus_tag="VEL74_02995"
     CDS             2331..>3066
                     /locus_tag="VEL74_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HYG61523.1"
                     /translation="MPLRFGINGLGRIGRALLRIAASRPGLEPVAVNDVVPAHVLARL
                     LARDTVHGPFPGGVGNVAAVEGRLVIGGRELPVFQEPDPARVGWGGAGAEVVVESTGR
                     FTRRELAAAHLRDGVRTVVVSANGEGMDATVCLGVNLGGGERDFDPARHAVISNASCT
                     TNCLALVAKVLHDSFGVRRALMSTVHSYTENQRLLDLPHPDPRRSRAAALNMIPTSTT
                     AAHSLGQVLPALAGRVDGYAVRVPTPA"
BASE COUNT          419 a         1047 c         1122 g          478 t
ORIGIN      
        1 ccgcgtccgc atctggccga gctgctcgcg cagcaaccgg cccaggccct tggcgtcccg
       61 ggcgcggatc ttcaccagga agcagtcctc gcccgcgatg tgatggacct cctgcacctc
      121 cgggatggcg gacaggcgct cggcggtctt gagctccccc gcaaagtcgt tggtggcgac
      181 gaagacgaac gccagcaggt ggtatcccag ggcctccggc gagacgcgcg tctcgtagcc
      241 ctggatcacc ccgctcgcct ccatccggcg gatccgctcc aggatcgccg acggcgccat
      301 ccccacctgc cgggcaatct cggcgttgga gatgcgggcg tttccctgaa ggatcgtcag
      361 gatttcacgg tctttggcgt cgatcattct cttcgcgcct cccaggaggg atgatactcc
      421 tcctggatgg ggtgtcaaga aggatcgtcg gtatagttca cagaaagacg ggggatcgtt
      481 ctctccggca tctttcctgg agaacaatcg ttggcgcggg cggccgttcc ggcctacatc
      541 gggatccggc tctcagcctc ggcggcgaag gggtcgtcgg cgaggccgcg gcggtggcgc
      601 aggagggcgg cgtgggcgat catggcggcg ttgtcgccgg cgaaggccat ggggaccagg
      661 cgcaggggga tgccgcgggc ctcggcccag gcggggagct cgcggcgcag cagccggttg
      721 gccgcgacgc cgcctgagac cgccagcagc tccagcggcc gctcccggtg caggcgctcc
      781 aggcggtcga gcacctgggc gacggtggcg gcgcggaagc cggccacgag gtccaggatg
      841 gaggcggagg gctcctggcg cgacaggtcg accggtccgg acacggcctc gatcctctcg
      901 atcgcctgca gggcctggct cttgaggccc gaataggaga agtcgaggct gtcgccgcag
      961 gagggggtgg ggaagcggtg cctcgacgcg tcccccctct cggccagctc gtccaccttg
     1021 gggccctggg ggtaggggag ccccaggcgc ttgcccacct tgtcgaagac ctcgcccatg
     1081 gcgtcgtcgc gggtctcggc gagggtcgag accgcgccgt cctccccctc tccgacggcg
     1141 aagagcgagc tgtggccgcc ggagagcacc aggccggtga agcgggcggg gggcgggccg
     1201 gccgggccgc cggcggcgtc gaggaagggg gagtagaggt gcccttccag gtgatggacg
     1261 gcgtggaacg gccgctccag gccgaaggcg atcgccttgc cgagggagag gccgacgagg
     1321 agcgccccca ccaggccggg gccgcgggtg gccgccacca cgtcgatgtc cgccagcggg
     1381 agaccggcgc gctccagcgc ctcgcggctc accagcggcc agttcgccag gtgctcgcgc
     1441 gaggcgatct ccggcaccac gccgccgtac ggccggtggg cggcgagctg gctcgacacg
     1501 accgaggcga gcacccggcc gtcgccgtcg aggacggcgc aggcggtgtc gtcgcaggag
     1561 gtctcgatgc ccaagacgag gggaacgacg aggggcggcg cgctcacgcg gggagtctag
     1621 cggatgcccc ctgaagaagg aaaaaccctg ccgcggcagg gttttcacct cacgttcgat
     1681 gcgtgcgggg gagaggcggg ttaggcgccg ccgccggccg gagcgtcgcc gctgccggcg
     1741 tcctcggcct tcgggatgtt gatcggctcg tagaccggga tctcgaacgc cagcgtgttg
     1801 tggcagtaat ccttccactc gtcggtgacg ttgtcgaaga tgatgttctc gtccttgaag
     1861 attccgtcgg tgacgacgta gttctcctgg aggtacagaa tggagtcgtc gttgagctcg
     1921 cgctcgacct cgacctcctt gccgttgacc tctttcttct ccttgcgcac gttggccgcg
     1981 ttctcggtcc agctcgggta ctcccggaac tgcttcgcga ggtacgcctt gcagtacttg
     2041 cccatctccg ccattgtcgg tcctcctacc ctgagggtgg tgtttcagat ctcggtttca
     2101 gatctcattt gtgcatcgcg ccggcgcccg gcctgcggcg gccggctcgt gtggaactgt
     2161 agcccagtcc ggtgggaagc cctactgatc ccggggtatt atccgcgacg cggagtccgg
     2221 acggaagctg ggcgcgttcc tctgctacca gtgcgcccaa tcccgaaagc gtgacaccgc
     2281 tgctagactg agcggctccg gtccgctcct cccggagaga gaacctgtcc atgcccctgc
     2341 gtttcggcat caacggcctc ggccgcatcg gccgcgccct gctgcggatc gccgcgtcgc
     2401 gccccggcct cgagccggtg gcggtcaacg acgtggtgcc cgcccacgtc ctcgcccgac
     2461 tgctcgcgcg cgacacggtc cacggcccct tccccggcgg tgtcggcaac gttgccgcgg
     2521 tcgaggggcg gctggtgatc ggcggccgcg agctgccggt gttccaggag ccggacccgg
     2581 cgcgggtggg gtggggcggc gccggggccg aggtggtggt ggagtccacc ggccgcttca
     2641 cccggcggga gctggcggcg gcccacctgc gggacggcgt gcgcacggtc gtggtctcgg
     2701 ccaacggcga ggggatggac gccacggtct gcctcggggt caatcttggg ggtggcgaga
     2761 gggacttcga cccggcccgc cacgcggtca tcagcaacgc ctcctgtacc accaactgcc
     2821 tcgccctggt ggcaaaggtg ttgcacgaca gcttcggggt ccgccgggcg ctgatgagca
     2881 cggtccacag ctacacggag aaccagcgcc tgctcgacct gccgcacccg gacccccggc
     2941 ggtcgcgggc cgcggcgctc aacatgatcc cgacgtccac cacggcggcc cacagcctgg
     3001 gccaggtgct cccggcgctc gccggccgcg tcgacggcta cgcggtgcgg gtgccgacgc
     3061 ccgccg
//
LOCUS       DASPJI010000112        12802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_5895, whole genome shotgun sequence.
ACCESSION   DASPJI010000112 DASPJI010000000
VERSION     DASPJI010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12802)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12802
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5895"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VEL74_03000"
     CDS             <1..347
                     /locus_tag="VEL74_03000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG61524.1"
                     /translation="SYASPMKILEYMAAGRAIVAPGAANVRELLTDGETALLCPGGEN
                     PTTEDLAAGVLALARDPELRARLGAAARRRIFERGYLWEENARRVEELVTGLQAEPSV
                     AVAVSEREARSC"
     gene            341..1171
                     /locus_tag="VEL74_03005"
     CDS             341..1171
                     /locus_tag="VEL74_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
                     /protein_id="HYG61525.1"
                     /translation="MLNAFSVDVEDYFQVGAFERHVPRQDWNRLEPRVERNTRRLLDL
                     CNEHGVKGTFFVLGWVAERWPGLVRDIRDAGHELGTHGQDHRRVTTLTPPQFREDVRR
                     SKGLIEDAAGTEVIGYRAPNYSIVRETMWAMDVLMEEGFRYDSSIFPIRHDRYGIPDF
                     PRFPRPVRGSNGSSLHEVPISTVRLAGMNLPFVGGGYLRQFPMSFIRWGMRRLNRIER
                     RPAVLYIHPWELDPEQPRFRVPFLTGVRHYRNLHLTESRLAELFGAYRFTTIREVLGL
                     "
     gene            1168..2310
                     /locus_tag="VEL74_03010"
     CDS             1168..2310
                     /locus_tag="VEL74_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HYG61526.1"
                     /translation="MSVEPLLLNNPLDNTLDPALSGPAGAATFETVAPRTDVLTVETL
                     RPELEARWDRYVRESPQATFFHQLGWRWLVERVFGHRAHYLLARRGERIAGVLPMFEM
                     KSLLFGHALVSLPFAIGGGIAADDAEATASLLAQAKSLADQRRVDYLELRSENAAAAD
                     LVTKDLYVTFRADLTEGEDALLKRMERKRRQMMSYVSKAGFSHRVAGIEELPLFYRMF
                     CDSMRYHGTPVYPRLFLHEILDRFPAQTNLFFVHEGGRPVAGVLNLLFGDVLMPFYAG
                     ADRSHRPRGVDDYLYLAIMRWGRENGYRVFDFGRSKRGTGAYKFKQRWGMEEVPLAYQ
                     YHLVRARELPNVSPANPKYQAVIRLWQKLPLPVTRLLGPMIVKRVP"
     gene            2313..3281
                     /locus_tag="VEL74_03015"
     CDS             2313..3281
                     /locus_tag="VEL74_03015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYG61527.1"
                     /translation="MSAGPHPDVSIVIPLYNEEESVDALHREVKAAADALERDYEIIL
                     VDDGSRDGTAEKLRAVCARDPRVKVVRFRRNYGQTAAIQAGFDHARGRVVITLDGDLQ
                     NDPADFGLLLAHIDEGYDVVSGWRRDRKDKMITRRLPSMAANWLIGKLTGMRIHDNGC
                     TLKAYRGEVVKRARLYAEMHRFLAPMMTLSGCRFKEVVVNHRARQFGRSKYGISRIWK
                     VFLDLLTIKMLLGFTTRPALWFGLLSLPFLVGTLLALIISVYIHVTLLPGMDYPIIFP
                     SVTILFAVAFVHLILLGVFSELVVKVGDFKESEAVLSALDSGRQAV"
     gene            3278..4345
                     /locus_tag="VEL74_03020"
     CDS             3278..4345
                     /locus_tag="VEL74_03020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG61528.1"
                     /translation="MRRARLHGDPDVSVVVPVVERYGDLRRLHDEFVREIEALGKSAE
                     FIFVLDERLRGALPALRALQQEAPDEVVIVLLGGSFGEAAALTLGLERARGEVVVTLA
                     AYFQVEARGIATAFAALEEGADMVVGRRFPRTDSGFNRLQSRLFHGILRGLTGSRFHD
                     ISCGFKVMRRRVSRELNLYGGLHRFIPVLALNRGFTVREVDIPQHPEDRRTRYHGTGA
                     YLGRLLDILTVFFLTKFTRMPLRFFGLMGAGLFALGFGVSLLAAVQKIFFDHGISDRA
                     VLLLGVLLMVLGVQTLSLGLIGEIIIFTHARNVRDYQVAEVIRNPVPRETAREVARET
                     AREAPRDRPREAPAAGALQAS"
     gene            4364..6343
                     /gene="asnB"
                     /locus_tag="VEL74_03025"
     CDS             4364..6343
                     /gene="asnB"
                     /locus_tag="VEL74_03025"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HYG61529.1"
                     /translation="MCGICGEWNLHGADRQALERMNDALIHRGPDDQGEVVLGETGLA
                     MRRLSIIDIARGHQPIANEDSTVWVVFNGEIYNHRELRRDLTARGHCFRTDSDTEVLV
                     HLYEERGERAVDELRGMFAFALWDTRRRRLLLARDRFGQKPLFYQFDGRRLLFASEIK
                     AILRATGQTPEVDLRSLDEYLTLRFIPSPRTLFTGIRKLAPAHLLILDAEGVHEREAG
                     DVREGGPQPAPKIQVRRYWRLSYLPKRRASEDEIVDEARHRIREAVESHLISDVPVGA
                     FLSGGMDSSLVVAMMSELLGEPVRTFAIGVEDQGFNELPFARVVAGHCRTDHHEEVVS
                     PDMVRLLPRMVYHLDEPSDPIAACMYHSAALAARHVKVVLTGDGGDEIFAGYDRYFGF
                     RWTGLYGALPAPVRRYLLGPALYAMPDKAGYKNLTQKARWVHELSFHEGGRRYAQATA
                     FFRFGQEGKGGIYTPEVVAELAGLDPMESVIRGFDDAEASDDLDRMLSADIATRMPEH
                     SLVLTDRMTMAQSLEGRSPLLDHRLAEFVATLPANMKLRGRTLKYLMRRVAASYLPET
                     ILKRPKQGFMFPLAAWIKGPLLPVLRRLIAEGAVIEAGIFRREALERLLAEHLANRAD
                     HHVRLWLVLNVEVWYRLFVRGEPLEELTGLLREAA"
     gene            6353..7396
                     /locus_tag="VEL74_03030"
     CDS             6353..7396
                     /locus_tag="VEL74_03030"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HYG61530.1"
                     /translation="MDVTQAGEQKHRSRFWPVMRVAVSAALVAWVLHRARWAEVAEAF
                     RAADLWPVLLALALNPIGYWASVSRWRLLLRTQGVDIPFGFLVRSFLVGVFFNNLLPS
                     TVGGDAMRAWESSRAGAGRSTAVAVVLVDRFLGLLTLMLLAGAGVLLSPRLTERVPSL
                     YAWVLAGALGMAFIAWLLFGRSARAESLLAWGLGRFPERWRPKLDGAARALLGFRGRW
                     PVLGKALAYSLVLQVAVVANGWLLAKALHVPIPLPYFFLIVPLAVFVMMIPVSINAIG
                     VRENVWAFFFTAFGVANAAAAGVAVAWLDYSLVLLQALVGGVLWMAGRRARREPVGEP
                     VAERPLEAGGAVR"
     gene            7393..8631
                     /locus_tag="VEL74_03035"
     CDS             7393..8631
                     /locus_tag="VEL74_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYG61531.1"
                     /translation="MRILLLAPHPFFQQRGTPIAERMLLEVLAANGHEIDVLTFAEGE
                     DVQIPGCRFLRIPRLPGTSGIRPGFSWKKLANDAVMLVESLRLIRRHRYNLVHAVEES
                     AFMALVGKWLFRVPYVYDMDSGLAQQMIDKFRPLGRVRPLLEVFERSAARGSAGVLAV
                     CRSLEEQARAWNPGGLVQRIEDVTLLEGNGKVLHLGTDLQIPLGLDGVRPPVVMYVGN
                     LEPYQGIDLLIEAFRQALRRAPRALLVLVGGAAGDIERYRERAERLGITASVHFAGPR
                     PVERLGSYLRQATVLVSPRIHGTNTPMKVYSYLDSGRPLLATRLPTHTQVLTDDVALL
                     VEPEPEAMSVGLVSLLEDEVLRERLADNARELAQREFTPQAFERKLLNFYDAVTEKTG
                     GTWIHGGETARLEAGRQSRR"
     gene            8582..10492
                     /locus_tag="VEL74_03040"
     CDS             8582..10492
                     /locus_tag="VEL74_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HYG61532.1"
                     /translation="MVERPRDSKLDVKAVAETLRRAEGAAGLAGALAEVDLDRLSAHD
                     YSVLSRALQGGLQEGGAPDVKIAYLSNFTVDLLPRYVDVCFAREGLRTASYVGGFDQY
                     VQEVLDEGSGLARFQPDLVFLTLSLRLLRPAPWASFSSLNADERRGLVLEVLDHLESW
                     ASAAVERLPGTVLIANFPAPACPGAGVADLKSEYGEAELFLELNLELVRRFKGKPRVQ
                     VFDLERLASCFGKDRVLDRKMYYLAKMEWSSAFLPAVAGELARHARAFQGRARKCVVL
                     DLDNTLWGGVVGEEGPAGVKIGPGDPEGEAYLDFHHRLKALQARGILLAVCSKNNPAD
                     ALEVFRTRPEMPLKVEDFAALEIGWEPKHQGLVRIAEALNIGVDSLVFVDDNPAEVSL
                     VAQMLPQVRVVQLPPDPAEYAATLDRLTEFEKPAILAEDLRKTEQYRENRAREELRSS
                     SGDLSGYLASLGTEVSVHRARHDDLARVHQLFTKTNQFNLTTRRYSPAEVERFACSPD
                     CELWAARARDRFGNLGMIAVVLLQREGRLVHVDSFLMSCRAMGRGIETAIMNHLKARL
                     QEDRNGYELRGRYLPTAKNKPVETFYEDQGFRLLDKKEDGEKLYSLRREDASPRPCGW
                     IQVVQDELVASR"
     gene            complement(10511..11461)
                     /locus_tag="VEL74_03045"
     CDS             complement(10511..11461)
                     /locus_tag="VEL74_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG61533.1"
                     /translation="MAQGSKQGQGKENKGGGTQVLEEEPQVQNEPQPPYPQQKLEKPG
                     IEAELELQPQWRAPKYKAAGKLEGKVALVTGGDSGIGRSVAYLYAREGADVAIVYLPA
                     EQQDADETKRAIEEEGRRCLTLPGDLTDAKFCREVVERTVRELGRLDILVSNAAWQDR
                     KESIEEITAEEWDRTLRTNIDAYFHLAKAAVPHMQPGSAIIATSSEVGLMGNKSLLDY
                     GATKGAINAFSKSLAQQLVPKGIRVNVVAPGPVWTPLNPADTGMKPENLEKFGSKQPM
                     ERAGQPEELAPAYVFLASDADSSYISGIVLEVMGGLTTGG"
     gene            11981..12490
                     /locus_tag="VEL74_03050"
     CDS             11981..12490
                     /locus_tag="VEL74_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61534.1"
                     /translation="MISTLTVEDVMARLERQVADHREQAGFHGGQEAHHRGQREQHTR
                     QAEAFAQHLQAFRAAAVPVVEDLTVAPSPVPLPALDALDAGARPRVQRLVWEVIQGFA
                     PGEPFGLSKVLDEIGRRYGAILDVPPDPGHVSVALRRLAKDGWILLVRRGRPRHESLY
                     VREGAEGGA"
     gene            complement(12569..>12802)
                     /locus_tag="VEL74_03055"
     CDS             complement(12569..>12802)
                     /locus_tag="VEL74_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYG61535.1"
                     /translation="VQGILEKDGEPLQPGRLLRLVAQDSTIRASALSTPGGSFSFPFL
                     APGQYTLLVDGTAVHSFALGEGSAEDLGRISVP"
BASE COUNT         1794 a         4350 c         4556 g         2102 t
ORIGIN      
        1 cctcgtatgc ctcgccgatg aagatcctgg agtacatggc cgccggccgc gccatcgtcg
       61 cgccgggggc cgccaacgtc cgcgagctgc ttaccgacgg ggagaccgcg ctgctctgcc
      121 cgggcggcga gaaccccacg acggaggacc tggcggccgg cgtcctcgcc ctcgcccgcg
      181 atccggagct ccgggcgcgt ctgggcgcgg cggcacggcg gcgcatcttc gagcgcggct
      241 acctgtggga ggagaacgcc cgccgcgtcg aggagctggt cacgggattg caagcagagc
      301 ccagtgtcgc agtggctgtg agtgaacggg aggctcgatc gtgctgaacg ctttttcagt
      361 cgacgtcgaa gattacttcc aggtcggcgc gttcgaaagg catgttccac ggcaggactg
      421 gaaccggctg gagccgcggg tggaacgcaa cacccggcgg ttgctggacc tttgtaacga
      481 gcatggggtc aagggcactt ttttcgtcct cggctgggtg gcggagcgct ggcccggcct
      541 ggtgcgcgac attcgcgatg ccggccacga gctggggacc cacggtcagg accatcggcg
      601 ggtgacgacc ctcaccccgc cgcagttcag ggaggacgtg cggcgctcca aggggctcat
      661 cgaggacgcc gcgggcaccg aggtcatcgg ctaccgcgcg ccgaactact cgatcgttcg
      721 ggagaccatg tgggcgatgg acgtgctgat ggaggagggc ttccgctacg actccagcat
      781 cttccccatc cgtcacgacc gctacggcat cccggacttc ccgcgcttcc cccggccggt
      841 gcgcggcagc aacggctcat cgctgcacga ggtgccgatc tcgacggtgc gcctcgccgg
      901 aatgaacctg ccgttcgtcg gcggcggcta cctgcgccag ttccccatgt ccttcatccg
      961 ctgggggatg cggcgcctca accggatcga gcggcgcccg gcggtcctct acatccatcc
     1021 ctgggagctc gacccggagc agccgcgttt ccgggtgccg ttcctgaccg gcgtccgtca
     1081 ctatcgcaac ctccatctca ccgagtcgcg gctcgccgag ctgttcggcg cctaccgctt
     1141 caccacgatc cgcgaggtgc tgggactatg agcgtcgagc cgttgctcct gaacaatccg
     1201 ttggacaaca ccctcgaccc ggcgctgagc ggtcccgcgg gtgccgcgac tttcgagacc
     1261 gtggcgccgc ggacggatgt cctgacggtg gagactctga ggcccgagct ggaggcgcgc
     1321 tgggaccgct acgtgcgcga gtcgccgcag gccaccttct tccatcagct cggctggcgc
     1381 tggctggtgg agcgggtctt cggccaccgc gcccactacc tgctggcgcg gcggggcgag
     1441 cgcatcgccg gcgtcctccc catgttcgag atgaagagcc tgctcttcgg ccacgcactg
     1501 gtgtcgctgc cgttcgccat cggcggcggc atcgccgcgg acgacgcgga ggcgaccgcc
     1561 tccctgctcg cgcaggcgaa gagcctggcc gaccagcgcc gcgtcgacta cctggagctg
     1621 cgctccgaga acgccgccgc cgccgacctc gtcacgaagg acctctacgt caccttccgg
     1681 gccgacctga ccgagggcga ggacgcgctg ctcaagcgca tggaacgcaa gcggcgccag
     1741 atgatgagct acgtgtcgaa ggcgggcttc tcgcaccgcg tcgccggcat tgaggagctg
     1801 ccgctcttct accggatgtt ctgcgacagc atgcggtacc acggcacacc ggtctacccg
     1861 cgtctcttcc tgcacgagat cctcgaccgc ttcccggcgc agaccaacct gttcttcgtc
     1921 cacgaggggg gccggccggt ggcgggggtg ctgaacctgc tcttcggcga cgtgctgatg
     1981 ccgttctacg ccggcgcgga ccgttcccac cggccgcgcg gcgtcgacga ctacctctac
     2041 ctggcgatca tgcgctgggg ccgggaaaac ggctaccgcg tcttcgattt cggccgcagc
     2101 aagcgcggca ccggcgccta caagttcaag cagcgttggg gcatggagga ggtgccgctc
     2161 gcctaccagt accacctggt gcgggcgcgg gagctgccca acgtcagccc ggcgaatccc
     2221 aagtaccagg ccgtgatccg cctctggcag aagctgcccc tgccggtgac ccggctcctc
     2281 ggaccgatga tcgtcaaacg ggtaccctag ccatgagcgc cggcccccac ccggacgtct
     2341 cgatcgtcat ccccctctac aacgaggagg agagcgtcga cgccctgcac cgcgaggtca
     2401 aggccgcggc cgacgccctg gagcgggact acgagatcat cctggtggac gacggcagcc
     2461 gcgacggcac ggcggagaag ctgcgcgccg tctgtgcccg cgacccgcgg gtgaaggtgg
     2521 tgcgctttcg ccgcaactat gggcagaccg ccgccatcca ggccggcttc gatcacgccc
     2581 gcggccgcgt cgtcatcacc ctcgacggtg acctgcagaa cgacccggcc gacttcgggc
     2641 tgctcctcgc ccacatcgac gagggctatg acgtggtctc cggctggcgg cgcgaccgca
     2701 aggacaagat gatcacgcgc cggctgccgt cgatggccgc caactggctg atcggcaagc
     2761 tcaccggcat gcgcatccac gacaacggct gcaccctcaa ggcctaccgt ggcgaggtgg
     2821 tgaagcgggc tcgcctctac gccgagatgc accgtttcct ggcgccgatg atgaccctgt
     2881 cgggctgccg cttcaaggag gtggtggtca accaccgggc gcggcagttc ggccgcagca
     2941 agtacggcat ctcgcgcatc tggaaggttt tcctcgatct gctgacgatc aagatgctgc
     3001 tcgggttcac cacccggccg gctctctggt tcggcctctt gagcctgccg ttcctggtgg
     3061 gcacgctgct cgcattgatc atcagcgtgt acatccacgt cacgctgttg ccggggatgg
     3121 attatccgat catctttcca tcggtgacga tcctgttcgc ggtcgcgttc gtccatctga
     3181 tcctgctcgg ggtcttctcg gagctggtgg tcaaggtggg cgacttcaag gagagcgagg
     3241 ccgtcctttc ggccctcgac tcaggaaggc aggcagtatg agaagagccc ggttgcacgg
     3301 cgatccggat gtttccgtcg tggtgccggt ggtggagcgc tacggcgacc tcaggcgttt
     3361 gcacgacgag ttcgtgcggg agatcgaggc gctgggcaag agcgcggagt tcatcttcgt
     3421 gctcgacgag cgcctgcgcg gtgcgttgcc ggcgctacgg gcgctgcagc aggaggcgcc
     3481 cgacgaggtg gtgatcgtgc tgctcggcgg cagcttcggc gaggcggcgg cgctcaccct
     3541 cgggctcgag cgggcgcggg gcgaggtggt ggtgaccctg gccgcctact tccaggtgga
     3601 ggcgcggggc atcgccaccg ccttcgccgc cctcgaggag ggggccgaca tggtggtcgg
     3661 ccgccgcttc ccacgcaccg actcgggctt caaccgcctg cagtcgcggc tcttccatgg
     3721 catcctgcgg gggctcaccg gcagccgctt ccacgacatc agctgcggtt tcaaggtgat
     3781 gcggcggcgg gtgtcgcgcg agctcaacct ctacggcggc ctccaccgct tcatcccggt
     3841 cctggcgctc aaccgcggct tcacggtgcg cgaggtggac atcccgcagc accccgagga
     3901 ccgccgcacc cgctatcacg gcaccggcgc ctacctggga cggctgctgg acatcctgac
     3961 cgtcttcttc cttaccaagt ttacccgcat gccgttgcgc ttcttcggcc tgatgggggc
     4021 cgggctcttc gccctcggct tcggcgtgtc gctgctggcc gcggtgcaga agatcttctt
     4081 cgaccacggc atctccgacc gggcggtgct gctgctgggc gtgctgctca tggtgctggg
     4141 ggtgcagacg ctgtcgctgg gtctgatcgg cgagatcatc atcttcaccc atgcccgcaa
     4201 cgtgcgcgac taccaggtgg ccgaggtgat ccgcaacccc gtccccagag agacggcgag
     4261 agaggtggcc agggagacgg cgcgggaggc gccgcgggac cggccgcgcg aggcgccggc
     4321 ggccggcgcc cttcaggcgt cctgagcgag gaggaagatc cggatgtgcg gcatctgcgg
     4381 tgagtggaac ctccacggcg ccgaccggca ggccctggag cggatgaacg acgcgctgat
     4441 ccaccgcggc cccgacgacc agggggaggt ggtcctcggc gagacggggc tcgccatgcg
     4501 gcgcctctcg atcatcgaca tcgcccgggg ccaccagccc atcgccaacg aggactccac
     4561 cgtctgggtg gtcttcaacg gcgagatcta caaccaccgc gagctgcggc gcgacctcac
     4621 cgcccgcggc cattgcttcc gcaccgacag cgacaccgag gtgctcgtcc acctctacga
     4681 ggagcggggc gagcgcgccg tcgacgagct gcgcgggatg ttcgccttcg ccctctggga
     4741 cacgcggcgg cggcggctgc tcctcgcccg cgaccgcttc gggcagaagc cgctcttcta
     4801 tcagttcgac ggccggcggc tgctcttcgc gtcggagatc aaggcgatcc tccgcgccac
     4861 gggccagacg ccggaggtgg acctgcggtc gctcgacgaa tacctgacgc tgcgcttcat
     4921 ccccagcccg cggacgctgt tcaccggcat ccgcaagctc gctccggccc acctgctgat
     4981 cctcgacgcc gaaggggtgc acgagcggga ggccggagat gttcgggagg gggggccgca
     5041 accggcgccg aagatccagg tccgccgcta ctggcgcctc tcctacctgc ccaagcgccg
     5101 cgcctccgag gacgagatcg tcgacgaggc gcgccaccgc atccgcgagg cggtggagtc
     5161 gcacctcatc tccgacgtgc cggtgggggc gttcctgtcc ggcggcatgg attccagcct
     5221 ggtggtggcg atgatgtccg agctgctcgg cgagccggtg cggaccttcg ccatcggcgt
     5281 cgaagaccag ggcttcaacg agctcccgtt cgcccgcgtc gtggccggcc actgccggac
     5341 cgatcaccac gaggaggtgg tgtcgcccga catggtgcgg ctcctgccgc gcatggtcta
     5401 tcacctggac gagccgtccg atcccatcgc cgcctgcatg tatcactcgg cggcgctggc
     5461 ggcgcgccac gtcaaggtgg tgctcaccgg cgacggcggc gacgagatct tcgccggcta
     5521 cgaccgctac ttcggcttcc gctggaccgg cctctacggt gcgctgcccg cgccggtgcg
     5581 gcgctatctg ctggggccgg cgctctatgc catgccggac aaggcgggct acaagaacct
     5641 gacgcagaag gcgcgctggg tgcacgagct gtcgttccac gaagggggcc ggcgctacgc
     5701 ccaggccacc gccttcttcc gcttcggcca ggaaggcaag gggggcatct acacgccgga
     5761 ggtggtggcg gagctcgcgg ggctggaccc gatggagtcg gtgatccgcg gcttcgacga
     5821 cgccgaggcc tccgacgatc tcgaccgcat gctctccgcc gacatcgcca cccgcatgcc
     5881 cgagcactct ctggtgctca ccgaccgcat gaccatggcg cagagcctcg aagggcgctc
     5941 gccgctgctc gatcaccggc tggcggagtt cgtggcgacc ctgccggcga acatgaagct
     6001 gcgtggccgg accctcaaat atctgatgcg gcgggtggcg gcctcctatt tgccggagac
     6061 gatcctcaag cggccgaagc agggcttcat gttcccgctc gccgcgtgga tcaagggccc
     6121 gctgctgccg gtgctgcgcc ggctcatcgc cgagggggca gtgatcgagg ccggcatctt
     6181 ccgtcgcgag gctctggagc ggcttctggc cgagcacctg gccaaccgcg ccgaccacca
     6241 cgtgcggctc tggctggtgc tgaacgtcga ggtctggtac cgcctgttcg tgcgcggcga
     6301 gcccctggaa gagctgacgg gactgctccg cgaggcggcc tagaggtccc gcatggacgt
     6361 gacgcaggcg ggtgagcaga agcaccgctc ccggttctgg ccggtcatgc gcgtggccgt
     6421 ctccgccgcc ctggtcgcct gggtgctcca ccgcgcccgc tgggccgagg tggcggaggc
     6481 cttccgtgcc gcggacctct ggccggtgct cctggcgctg gcgctcaacc ccatcggcta
     6541 ctgggccagc gtcagccgct ggcgcctgct gctgcgcacc cagggggtgg acatcccgtt
     6601 cggcttcctc gtccgctcct tcctggtggg ggtcttcttc aacaacctgc tgccctcgac
     6661 cgtcggcggc gacgccatgc gcgcctggga gtcctcgcgg gcgggggcgg ggcgttcgac
     6721 ggcggtggcg gtggtgctgg tggaccgctt cctcggcctg ctgacgctga tgctcctggc
     6781 gggcgccgga gtcctgctgt cgccgcggct gacggagcgg gtgccgtccc tctacgcctg
     6841 ggtcctggcg ggcgcgctgg ggatggcctt catcgcctgg ctgctcttcg gcaggtcggc
     6901 gcgggcggag tccctgctgg cgtgggggct ggggcggttc ccggagcgct ggcgcccgaa
     6961 gctcgacggc gcggcgcggg cactcctggg gttccgcggc cggtggccgg tgctgggcaa
     7021 ggcgctggct tactcgctgg tcctccaggt ggcggtggtg gcgaacggct ggctgctcgc
     7081 caaggcgctg cacgtgccga tcccgctgcc gtacttcttt ctcatcgtgc cgctggcggt
     7141 cttcgtgatg atgatcccgg tgtcgatcaa cgccatcggc gtgcgggaga acgtctgggc
     7201 cttcttcttc accgccttcg gcgtcgccaa cgcggcggcg gcgggtgtgg ccgtggcctg
     7261 gctcgactac agcctggtgc tgctccaggc cctggtgggc ggcgtgctct ggatggcggg
     7321 ccggcgggcg cggcgagagc cggtgggaga gccggtggcg gaaagaccgc tcgaagccgg
     7381 aggggccgtc cgatgaggat cctgctcctc gccccgcatc cgttcttcca gcagcgcggc
     7441 acgcccatcg ccgagcgcat gctcctcgag gtgctcgccg ccaacggcca cgagatcgac
     7501 gtgctgacct tcgcggaagg ggaggacgtc cagatccccg gctgccgctt tctccgcatc
     7561 ccgcgcttgc ccggcacctc gggtatccgc ccaggcttct cgtggaagaa gctggccaac
     7621 gacgcggtga tgctggtcga gtcgctgagg ctcatccgcc gccaccgcta caacctggtg
     7681 cacgcggtgg aggagtcggc cttcatggcg ctggtgggca agtggctgtt ccgcgtcccc
     7741 tatgtctacg acatggattc agggctcgcg cagcagatga tcgacaagtt ccggccgctc
     7801 ggccgggtgc gccccctgct cgaggtgttc gagcgctcgg cggcccgggg gagcgccggc
     7861 gtcctggcgg tctgccggtc gctggaggag caggcccggg cctggaaccc cggcgggctg
     7921 gtgcaacgca tcgaagacgt gacgctgctc gaggggaacg gcaaggtgct ccacctgggg
     7981 accgacctcc agatccccct agggctcgat ggcgtgcgcc cgccggtggt catgtacgtg
     8041 ggcaacctgg agccctatca ggggatcgac ctgctgatcg aggcgttccg gcaggcgttg
     8101 cggcgggcgc cgcgggcgct gttggtgctg gtcggcggcg ccgccgggga catcgagcgc
     8161 taccgcgagc gggccgagcg gctgggcatc acggcgagcg tccactttgc cgggccgcgg
     8221 ccggtggagc ggctcggctc ctacctgcgg caggcgacgg tcctggtctc cccccgcatc
     8281 cacgggacca acacgccgat gaaggtctac tcgtacctgg attcgggccg gccgctgctg
     8341 gcgacgcgcc tgcccaccca cacccaggtg ttgaccgacg acgtggcgct gctggtggag
     8401 ccggagcccg aggcgatgag cgtgggcctc gtctcgctcc tcgaggacga ggtcctgcgc
     8461 gagcgtctgg ccgacaacgc ccgcgagctc gcccagcgcg agttcacgcc gcaggcgttc
     8521 gagcgtaagc tgctaaactt ctatgatgcc gtcacagaaa agaccggagg gacctggatc
     8581 catggtggag agaccgcgcg actcgaagct ggacgtcaaa gccgtcgctg agacgctgcg
     8641 cagggcagag ggtgccgccg gcctggccgg ggcgctggcg gaggtggacc tggaccggct
     8701 ctcggcccat gactacagcg tgctctcgcg ggcgctccag ggcgggctcc aggagggcgg
     8761 agcaccggac gtgaagatcg cctacctgtc caacttcacc gtcgacctgc tgccgcgcta
     8821 cgtggacgtc tgcttcgcgc gcgaagggct gcggacggcg agctacgtcg gcggcttcga
     8881 ccagtacgtc caggaggtgc tggacgaggg ctcgggcctg gcgcggttcc agccggacct
     8941 ggtgttcctg accctgtcgc tgcggttgct gcgcccggcg ccgtgggcga gcttctcgtc
     9001 cctgaacgcg gacgagcgcc gcggcctggt gctggaggtg ctggaccacc ttgagtcgtg
     9061 ggcgtcggcg gcggtggagc ggctgcccgg cacggtgctc atcgccaact tcccggcgcc
     9121 tgcctgtccg ggggccgggg tcgcggacct caagtcggag tatggcgaag ccgagctctt
     9181 cctcgagctc aacctggagt tggtgcgccg gttcaagggg aagccacggg tgcaggtctt
     9241 cgatctggag cgcctcgcct cctgcttcgg caaggaccgg gtgctcgacc gcaagatgta
     9301 ctacctggcg aagatggagt ggagctcggc cttcctgccg gcggtggcgg gggagctggc
     9361 gcgccacgcc cgggcgttcc aggggcgggc ccgcaagtgc gtggtgctcg acctcgacaa
     9421 caccctctgg ggcggagtgg tgggggagga ggggccggcg ggggtcaaga tcggcccggg
     9481 cgatcccgag ggggaggcct acctcgattt ccatcaccgc ctcaaggcgc tccaggcgcg
     9541 cggcatcctg ctcgcggtgt gcagcaagaa caacccggcg gacgcgctgg aggtcttccg
     9601 tacccggccc gagatgccgc tcaaggtgga agacttcgcc gccctggaga tcggctggga
     9661 gccgaagcac caggggctcg tccgcatcgc cgaggcgctg aacatcggcg tcgacagcct
     9721 ggtgttcgtg gacgacaacc cggccgaggt cagcctggtg gcgcagatgc tgccgcaggt
     9781 gcgggtggtg cagcttccgc cggacccggc ggagtacgcg gcgacgctcg accggctgac
     9841 cgagttcgag aagccggcga tcctggccga ggacctgcgg aaaaccgagc agtaccggga
     9901 gaaccgggcg cgcgaggagc tgcggtcgtc gtccggtgac ctgtcgggtt atctcgcgag
     9961 cctgggcacc gaggtgtcgg tccaccgcgc ccgccacgac gacctggcgc gggtgcacca
    10021 gctcttcacc aagaccaatc agttcaatct gaccacccgc cgctactcgc cggccgaggt
    10081 ggagcgcttc gcctgctcgc cggactgcga gctatgggcg gcgcgggcgc gggaccgctt
    10141 cggcaacctg gggatgatcg cggtggtgct cctgcagcgg gaggggcggc tggtgcacgt
    10201 cgacagcttc ctgatgagct gccgggcgat ggggcgcggc atcgagaccg cgatcatgaa
    10261 ccacctgaag gcccgcctcc aggaggaccg caacggctac gagctgcgcg gccgctatct
    10321 ccccacggcg aagaacaagc cggtggagac cttctacgaa gaccagggat ttcgtctgct
    10381 ggataagaaa gaggacggcg agaagctcta ctccctgcgc cgggaggacg catcgccccg
    10441 cccctgcggc tggatccagg tcgtccagga cgagctggtg gccagccggt aggaggcggg
    10501 gcggagaggc tcatcccccg gtggtcaacc cgcccatcac ctcgagcacg atgccgctga
    10561 tgtacgagga gtcggcgtcg ctggccagga agacgtaggc cggggcgagc tcctcgggct
    10621 ggccggcgcg ctccatcggc tgcttgctgc cgaacttctc caggttctcg ggcttcatac
    10681 ccgtgtccgc ggggttgagc ggcgtccaca ccgggccggg ggccaccacg ttgacgcgga
    10741 tgcccttggg gacgagctgc tgcgccagcg acttggagaa agcgttgatc gcccccttgg
    10801 tcgcgccgta gtcgagcagc gacttgttgc ccatcaggcc cacctcggag ctggtggcga
    10861 tgatggcgct gcccggctgc atgtgcggga cggcggcctt ggccaggtgg aagtaggcgt
    10921 cgatgttggt gcgcagcgtg cggtcccact cctccgcggt gatctcctcg atgctctcct
    10981 tgcggtcctg ccaggcggcg ttggagacga ggatgtccag ccggccgagc tcgcgcacgg
    11041 tccgctcgac cacctcacgg cagaacttcg cgtcggtgag gtcgccgggg agggtgaggc
    11101 accgccgccc ctcctcctcg atggcccgct tcgtctcgtc cgcgtcctgc tgctccgcgg
    11161 gcagatagac gatcgccacg tccgcccctt cgcgggcata gaggtaggcg acggaccggc
    11221 cgatgccgct gtcgccgccg gtcaccagcg ccaccttgcc ctccagcttg ccggcggcct
    11281 tgtacttcgg ggcgcgccac tgcggctgca gctccagctc cgcctcgatc cccggcttct
    11341 ccagcttctg ctgcggatac ggcggctgcg gctcgttctg gacctgcggc tcctcctcca
    11401 gcacctgcgt gccgccgccc ttgttctctt tgccctgtcc ctgcttcgat ccctgcgcca
    11461 tgatgtgttc ctccttcggt agtaacgggg tcgcgagccg ccgtcccacc cccctgaggt
    11521 gcaagagcag tacccgtctg aacgtcttga cccccttgat tcgtataaag aggtaggcgt
    11581 tccgtatccg gacggtttcg cgacggcaga ggggtgattc gcgacggtga aggcttccgg
    11641 cgtcgcgaat cacccgtcat ccgtcgcgag tgcggagaag gtcgtcgcga aacgcgcgag
    11701 atcggtcgcg agctcgaggg ttgccgtcgc gagtcccgcg gcggcggatt cggcggttgc
    11761 gaatcgcgtc tcgtatctcg cagccgccgt tccgaatgcc gccggtgtcg cgacaaaccg
    11821 tggagccgct gtcgcgagtg aaggtccatt cgcgacggcg gagacgggtt ctgtttcgta
    11881 tctcccgacg gcgtcttcag ctctcgagag ccatgttgcg tatctccctg gactcgcgac
    11941 cgggtaggcg aagggggagc gtatagaaga ggagagaagg atgatttcga cgctgacggt
    12001 agaggatgtg atggcccggc tggagaggca ggtggccgac caccgggagc aggccgggtt
    12061 tcacggcgga caggaggcgc atcaccgcgg ccagcgggag cagcacaccc gccaggccga
    12121 ggcgttcgcg cagcatctcc aggccttccg cgcggccgcg gtgccggtgg tggaggatct
    12181 cacggtggcg ccgtcgccgg tgccccttcc cgcgctcgac gccctggacg ccggtgcgcg
    12241 tccacgggtg cagcggctgg tctgggaggt gatccagggc ttcgcgccgg gtgagccctt
    12301 cggcttatcc aaggtgctcg acgagatcgg gagacgctac ggcgccatcc tcgacgtgcc
    12361 gcctgatccc ggccacgtct ccgtcgccct gcggcggttg gccaaggacg gctggatcct
    12421 tctggtgcgc cgcggccggc cacgccacga gtcgttgtat gtgcgggagg gggcggaggg
    12481 cggcgcctag gagcgttctg ctgttccggc gagactggcc gggtccgacg aaagtggcgc
    12541 cctcgccggt tcctgcgctc gcgctctttt aggggacgga gatccgcccc agatcctcgg
    12601 cgctgccctc gccgagcgcg aaggaatgga ccgccgtgcc gtccacgagc aaggtgtact
    12661 ggccgggggc gaggaagggg aaggagaagc tgccgcccgg ggtggagaga gcggaggcgc
    12721 ggatggtcga gtcctgcgcc accaggcgga gcagccggcc gggctggaga ggctcgccat
    12781 ccttctccag gattccctgg ac
//
LOCUS       DASPJI010000113         9998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_238925, whole genome shotgun sequence.
ACCESSION   DASPJI010000113 DASPJI010000000
VERSION     DASPJI010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9998)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9998
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238925"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3056)
                     /locus_tag="VEL74_03060"
     CDS             complement(<1..3056)
                     /locus_tag="VEL74_03060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61536.1"
                     /translation="MSPETPLSPQQRHIWRLQTDSRAYRSQCTVLLCGDLDAEALRRA
                     VGEAVARHEALRTTFRRSPGVRLPTPLLAAEPPAPLWQVADLSQRPEQERAAALDDLE
                     RQADRRPFDLERGPLVHAVLGRLADGLHALVLGAPALCADGASLDVLAREVAVGYAGE
                     HPAGEPLQYGAAAHWLNETLAAEESRAGRDHWRAQGRPERFALRLPFETRAGAGFAPD
                     RVAVPLEPDSASRAKAFAAGCWAELGDLLLAAWQVLLWRLDGEAPGAGVRFDGRPYEE
                     LWEAVGPYARWLPFPSRVAPGLSFTSLVDALRAEREEGADWQVAYAAEERAAAGIHAC
                     FDLWERPAAITSRGVAFEPARRSAWGERFKVCLAVTRDAAGLAAELWFDSHLLAPEHA
                     RPQAERFARLLAALLEAPERQIAEAPAVSAEEVAQILAWQGAAVSYPVDACLHRLIEA
                     KVERTPGAVALEFEGEELTYRQLDERANRLARRLRALGAGPEARVAVCLERSLELVVS
                     LLAVLKAGAAYVPLDPGYPPERLAYMLEDSAAAVLVTDERLLAALLPTETAGEGRRPA
                     VPTLRVDAEREAIERESAAPLPDAPPAEALAYAIYTSGSTGRPKGAMNSHRAIINRLL
                     WMQDTYGLTAGDRVLQKTPASFDVSVWELFWPLLAGARLVVARPGGHQDPAYLIDVIS
                     RRGITTLHFVPSMLLAFLDSPGVEACTGSLRRVICSGEALTVNLADAFFQRLDDVELH
                     NLYGPTEAAVDVTAWHCDRERSRHTVPIGHPVANTSILLLDADRRPVPPGSPGELYIG
                     GAQVGRGYLGRPDLTAERFVPDPFGAASGGGRLYRTGDLARYEADGAVDFLGRVDHQV
                     KVRGFRIELGEVETALTSHPDVREAAVLAREENPGERRLVAYYVPRERTPSVDDLRRF
                     LGSRLPDFMVPALFVRLAALPLNPNGKLDRVALPAPGSQRPDLEHEYVAPGSPVEQAL
                     AEVWSEVLGIEAIGVHDSFFALGGDSIRSVRVAALAKERGIEMAVE"
     gene            complement(3072..7607)
                     /locus_tag="VEL74_03065"
     CDS             complement(3072..7607)
                     /locus_tag="VEL74_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61537.1"
                     /translation="MSGTLEQVALQGFPLSPQQRRLWSLRGDRDGAPYRASCAVLLEG
                     ALDRRRLRAALERVVEHHESLRTTFRCLPGMTVPLQIVGEERRFAWGEGGAAMAALER
                     PWSLGEEPPLRAVLIPDGPQRHRLILALPALCADARSLANLVEEIATAYGSAGSAEPA
                     GDLMQYADVAAWQNEVLDAEETEAERDFWRQQEVASLLAEPLPFERAAEEGFAPRTAA
                     AGLDLGPGLARAAPEAFLLACWQVLIGRLTGRSELVVCARSEGRCFAELAAVVGPLEK
                     FLPVVLRWEEGERFAQIARRAGRALEQARAHQNAFTWDRLAPEGGLAPSLAFEVESPL
                     PLGGAGGLHWTLQRASAMTERFALKLSCTVEPGGSLRATLHYDASRFLETDVHHIAEW
                     LTVLARQAAAAPESAAGELELLTGRERRRELVEHNSTRMELPRRLLHELVREQAARTP
                     GKVAIRSESGTLTFAELVERTGRLASRLRALGVGPEVPVAVHAERSPEMVVALLAILE
                     AGGAWVPLDPDHPAERLAGILQDVEPAAVLTQERLAGSLPSHAGMVVLLEEALHPLAA
                     SLIPSPPAGEEVDDRLAYVIYTSGSTGRPKGVMVSHRAIVNRLLWMQCELPLAPDDRV
                     LQKTPYGFDASIWEIFCPLLAGAELVLARPGGHQDSAYLAGAVAEQRVTVLQLVPSLL
                     GPFLDEPAAAECRSLRRMFCGGEELTVDLARRFSSLLPDAVLHNLYGPTEAAIDATFH
                     VVSEAPAGLSVPIGRPIANLEVYLLSPRGMPVPAGVAGELCIGGTGLARGYWRRSDQT
                     AERFVPHPFSGETGACLYRTGDLARRRPDGAIEYLGRADRQVKVRGVRIEPREIEAVL
                     TGHPEVRQAVVLPVPESEGNGVRLAAFVMPEVETAGLRRFLGERLPAAMMPAVLVPLR
                     TLPLNPNGKLDTRALADAAREASEAGRQSGYVAPRTPIEQVLAEVWAEILGVERVGLR
                     DDFFGLGGHSLRATQLVSRVRRVFQVELPVRDLFERPTVEALAAHLDRLLAGGGAGAT
                     PPIVPVPRSGSLLLSFAQQRLWFLEQLRPGTPLYNIPDAVRFRGPLDVAALATALTEV
                     VRRHEPLRTAFPTVRGEAVQRIDPPGPLSLPLVDLSALPPGRRGEEAARRTDEEGARP
                     FDLARGPLLRPVLFRLDVENHRAAFTVHHIVSDAWSSGVLVRELTVLYQAARQGEPSP
                     LPELPVQYADFAAWQRRWLEGGALEPQLAWWREHLAGAPPVLDLLADRPRPERPSYRG
                     ATRLVLLPATLTDGLRALGRREGATLFMVVFAAFAVVLRRLAGRDDIVAGTDVANRNR
                     AETEGMIGFFVNQLVLRVDLSEDPTFLAVLARVRAAALGAFTHQDVPFETLVDRLQVE
                     RSAAYAPLFQVKLFLENTPAGAFEMPELALERLDTAVHIAKLDLTLALWERPEGLSGW
                     LSYNTDLFDEPRMVRLVRLLATTAAAAAAWPGARLSEIDGVLDELERRERESQKVELA
                     KSSFQRFQTLVQKKDPK"
     gene            complement(7604..>9998)
                     /locus_tag="VEL74_03070"
     CDS             complement(7604..>9998)
                     /locus_tag="VEL74_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG61538.1"
                     /translation="LYGPTECAIDATFWRCRPDDPSVPIGRPIANDRAYLLDASLRPV
                     PAGEVGELYVAGTGLARGYLDRPDLTAERFLPDPFAVEPGGRLYRTGDLTRHVPPGQA
                     LAGALEYAGRADDQVKVRGVRIELGEVESWLVRHPGVAEAVAGVRDGGAGPEDRRLIA
                     WVVLRAAAPAATDLRAFLAERLPDAMVPSAFVTMPALPRLASGKVDRAALPAPSTCSE
                     ASSAALRTPTEEVLAVLWAEVLGVERLGRQDNVFTLGWHSLLATQLVTRLRRAFGVDV
                     PLRSLFEHPTVAALAERIDAALRGGPGFAAPPMERSPRGTHPLLLSFAQQRLWFLDRL
                     APGNPFYNVPVTLRLTGALDPGALARSFSEVARRHEVLRTTFPEVDEQPVQSVAPPAP
                     VALPLADLLALPETARQVEARRLDAEEALRPFGLARGPLLRATLVRLSAEEHLLLLTM
                     HHIVADGWSSQVLTQEVREVYGALLAGRRPDLPALPLQYADYARWQREWLRGAVLDAH
                     VGYWRRQLAGAPPALELPTDRPRPAVQSFRGATRSRLLSPEVAAAIRELAGYEGVTLF
                     MALLAAAQVLLGWSAGQDDVVVGTDVAGRDRPETEGLIGFFINQVALRTRLGGDPTFR
                     ELLARVRETMLGAYAHQELPFDKLVEALNPERSLGRSPVFQVKVNLLNLPPAALELPG
                     LRIEPQRPVRETAQFDWILNLADSEQGLLAWVEHATDLFDAATIDRMLGNFEALLRAV
                     AGRPELRLAEMAEALEESDAAQRLAAQEGKRQGLQDRLRRSRRTAPVGVPFAEEAVR"
BASE COUNT         1450 a         3650 c         3675 g         1223 t
ORIGIN      
        1 tcctccaccg ccatctcgat cccccgctcc ttcgccaggg ccgccacccg gacactgcgg
       61 atggagtcgc cgcccagggc gaagaagctg tcgtgcacgc cgatggcctc gatccccagt
      121 acctcgctcc agacctcggc cagcgcctgc tccaccggcg agccgggggc cacgtactca
      181 tgctccaggt cggggcgctg ggagccgggt gccggcaggg cgacgcggtc gagcttgccg
      241 ttggggttga gcggcagggc cgccaagcgg acgaacagcg ctggcaccat gaagtccggc
      301 aggcgcgagc cgaggaaccg ccgcaggtcg tcgacgctgg gggtgcgctc gcgggggacg
      361 tagtaggcca ccaggcgccg ctctccgggg ttctcctcgc gggcgagcac cgccgcctcg
      421 cgcacgtccg ggtgggacgt aagcgccgtc tccacctccc ccagctcgat gcggaagccc
      481 cgcaccttga cctggtggtc gacgcggccg aggaagtcca cggctccgtc cgcctcgtag
      541 cgggcgaggt cgccggtgcg gtagagccgg ccgccgccgg aagccgcgcc gaacgggtcg
      601 gggacgaagc gctccgccgt caggtccgga cgacccaggt aaccccgtcc tacctgcgcg
      661 ccgccgatgt acagctcgcc cggggagccg ggcggcaccg gccggcggtc ggcgtcgagc
      721 aggaggatgg aggtgttcgc caccggatgg ccgatgggga cggtgtggcg gctccgctcg
      781 cggtcgcaat gccaggcggt cacgtccacc gccgcctcgg tggggccgta gaggttgtgg
      841 agctccacat cgtcgagacg ctggaagaag gcgtccgcca gattgacggt cagcgcctcg
      901 ccgctgcaga tcacccgccg caggctgccg gtacacgcct cgacgccggg ggagtcgagg
      961 aaggccagca gcatggaagg gacgaagtgg agggtggtga tcccccggcg gctgatgaca
     1021 tcaataagat aggcggggtc ctgatggccg cccggccggg ccaccaccag tcgggccccg
     1081 gccagcagcg gccagaagag ctcccagacc gagacgtcga agctcgccgg cgtcttctgg
     1141 agtacccggt cgccggcggt caggccgtag gtgtcctgca tccagagcag gcggttgatg
     1201 atggcgcggt ggctgttcat cgcccccttg ggccggccgg tggagcccga ggtgtagatc
     1261 gcgtaggcga gggcttcggc gggtggcgcg tcagggaggg gtgcagcgct ctccctctcg
     1321 attgcctcgc gctccgcgtc gacgcggagg gtgggaacgg ccggccgcct cccctccccc
     1381 gccgtctccg ttggcagcaa ggccgccagc aggcgctcgt cggtgactag gacggcggcg
     1441 gcggagtcct ccagcatgta ggccaggcgc tccggcggat agccggggtc cagggggacg
     1501 taggcggcgc cggccttgag gaccgccagc agcgagacca ccagctcgag cgagcgctcc
     1561 aggcaaacgg cgacgcgcgc ctcagggccc gctcccagcg cccgcagccg gcgcgccagg
     1621 cggttggccc gctcgtcgag ctggcgatag gtcagctcct ccccctcgaa ttcgagcgcc
     1681 acggcgcccg gggtgcgctc caccttggcc tcgatcaggc ggtgcaggca ggcgtccacc
     1741 ggatagctca cagccgctcc ctgccaggcg aggatctgcg ccacctcctc ggcactgacc
     1801 gccggtgcct cagcgatctg ccgctccggc gcctccagga gcgcggcgag aaggcgggcg
     1861 aagcgctccg cctgcggccg ggcgtgctcc ggcgccagca ggtgggagtc gaaccacagc
     1921 tccgccgcga ggccggcggc gtcgcgcgtc accgccaggc agactttgaa gcgctctccc
     1981 caggcgctgc ggcgcgccgg ctcgaaggcg acgccgcggg aagtgatcgc cgccggccgc
     2041 tcccagagat cgaagcaggc gtggatgccc gccgccgccc gctcctcggc ggcataggcc
     2101 acctgccagt ctgccccctc ctccctttcg gcgcgtagcg catctaccag ggaagtgaag
     2161 gagaggcccg gcgccacccg ggaggggaac ggcaaccagc gggcgtaagg ccccacggct
     2221 tcccacagct cctcgtaggg ccggccatcg aagcgcacgc cggctcccgg cgcctcgccg
     2281 tccagacgcc agaggagcac ctgccaagcg gcgagcagga ggtcgccgag ctcggcccag
     2341 cagccggcgg caaaggcctt ggcccggctc gcgctgtctg gttccagcgg taccgccacc
     2401 cggtccgggg cgaagccggc gcccgccctc gtctcgaaag ggaggcgcag ggcgaagcgc
     2461 tcgggtcgcc cctgcgcgcg ccagtggtcg cgcccggcgc ggctctcctc ggcggccagc
     2521 gtctcgttga gccagtgggc ggcggcaccg tactgtaacg gctcgccggc cgggtgctcg
     2581 ccggcgtagc cgacggccac ctcccgcgcc agcacgtcca ggctggcgcc gtcggcacag
     2641 agggccggcg cgcccagaac cagggcgtgg agaccgtccg ccagccggcc caggacggcg
     2701 tgcaccagcg gcccccgttc gaggtcgaac ggccggcggt cggcttggcg ctccaggtcg
     2761 tccagcgccg ccgcgcgctc ctgctcggga cgctgcgaca ggtcggccac ctgccagagc
     2821 ggggccggcg gctcggccgc caacagcggc gtgggcaggc ggacgcccgg agaacgccgg
     2881 aaagtggtgc gtagcgcctc atggcgcgcc acagcctctc ccaccgcgcg gcgcagcgcc
     2941 tcggcgtcga gatccccgca cagcaggacg gtgcactggg accggtaggc gcggctgtcc
     3001 gtctgcaggc gccagatgtg acgctgctgg ggcgagaggg gggtctcagg cgacatcggg
     3061 cctccttgct cctacttcgg gtctttcttc tggacgagcg tctggaagcg ctggaagctg
     3121 gacttcgcca actcgacttt ctggctctcc cgctccctgc gctcaagctc gtcgagtacg
     3181 ccgtcgatct cgctcagccg cgctccgggc catgccgcgg cggccgctgc ggtggtggcg
     3241 aggagccgga ccagacggac catccgcggc tcgtcgaaca ggtcggtgtt gtagctcagc
     3301 cagccgctca gcccctccgg ccgctcccaa agggccaggg tgaggtcgag cttggcgatg
     3361 tggaccgccg tgtcgaggcg ctccagcgcc agctccggca tctcgaacgc gcccgcaggg
     3421 gtgttctcca ggaagagctt gacctggaac agcggcgcgt acgcggcgct acgctccacc
     3481 tgcagccggt ccaccagggt ctcgaagggc acgtcctggt gggtgaaggc gccgagggcg
     3541 gcggcgcgga cccgcgccag caccgccagg aaagtcgggt cctccgacag gtcgacgcgc
     3601 agcacgagct ggttgacgaa gaagccgatc atcccttcgg tctcggcgcg gttgcggttg
     3661 gcgacgtcgg tgccggcgac gatgtcgtcc cggccggcga ggcggcgcag gaccacggcg
     3721 aaggcggcaa agaccaccat gaagagggtg gccccctcgc gccgtcccag cgcccgcaga
     3781 ccgtcggtga gcgttgcggg cagcagcacc aagcgggttg cgccgcggta ggaggggcgc
     3841 tccggccgcg gccggtcggc cagcaagtcg aggaccggcg gagcgccggc gaggtgctcc
     3901 cgccaccagg cgagttgggg ctccagcgca cccccttcga gccagcggcg ctgccaggcg
     3961 gcgaagtcgg catactgcac gggcagctcg ggcagggggg acggctcgcc ctgccgcgcc
     4021 gcctgataga gaacggtcag ttcccgtacc agcacgccgc tcgaccaggc atcgctcacg
     4081 atgtggtgca cggtgaacgc ggcgcggtga ttttccacgt ccagccggaa caggacgggg
     4141 cggagcagcg gcccccgcgc caagtcgaac gggcgtgccc cctcctcgtc cgtgcggcgg
     4201 gccgcctcct cgcctcggcg gcccggcggc agcgcggaga ggtccacaag ggggagggag
     4261 agcggtcccg gcgggtcgat gcgctgcacg gcctcgcccc gcactgtcgg gaacgcggtg
     4321 cgcaacggct cgtgccgcct cacgacctcg gtcagcgccg tggcgagggc ggcgacatcc
     4381 agcggtccac ggaagcgcac cgcgtccggg atgttgtaga gcggcgtgcc cggccggagc
     4441 tgctccagga accagagccg ctgctgggcg aaggagagca gcagggaccc ggagcggggc
     4501 accggcacga tcggcggcgt cgccccggcg ccgccccccg ctagcaaccg gtcgaggtga
     4561 gcggcgagag cctcgaccgt cgggcgctcg aacaggtcgc gcaccggcag ctcgacctgg
     4621 aagacccggc gcacccgcga gaccagctgg gtggcgcgca acgagtggcc gccgaggcca
     4681 aagaaatcgt cgcggagccc cacccgctcg actcccagga tctcggccca gacctccgcc
     4741 agcacctgct cgattggggt ccgcggtgcc acgtagccgg actggcgccc ggcctcggag
     4801 gcctcccgcg ccgcgtcggc cagggccctg gtatcgagct tgccgttggg attgaggggc
     4861 agcgtccgca ggggcacgag gaccgccggc atcattgccg ccggcagccg ctcccccaga
     4921 aagcggcgca ggccggctgt ctcgacctct ggcatcacga acgcggccag gcgcactccg
     4981 ttcccctcag actccggcac cggcagcacc accgcctgcc gcacctccgg gtgaccggtg
     5041 agcacggcct cgatctcccg cggctcgatg cgcacgccac gcaccttgac ctggcggtcg
     5101 gcccggccca ggtactcgat ggcgccgtcc ggccgccgcc gcgccaggtc gcccgtgcgg
     5161 tagaggcagg cccccgtctc gccagagaag ggatggggga cgaaccgctc cgccgtctgg
     5221 tccgaccgcc gccagtagcc acgggcgagg cccgtcccac cgatgcacag ctcccccgcc
     5281 acccccgcgg gcaccggcat gccgcggggg ctcagcaggt agacctccag gttggcgatg
     5341 ggccggccga tgggcacgct cagcccggcc ggcgcttcgc tgaccacgtg gaaggtggcg
     5401 tcgatagccg cctccgtcgg tccgtagagg ttgtgcagga cggcgtccgg cagcagcgat
     5461 gagaagcgcc gcgccaagtc gaccgtcagt tcctcgccgc cgcagaacat ccgccgcagc
     5521 gaccggcact ccgccgcggc cggctcgtcg agaaatggcc ccagcaggga gggcacgagt
     5581 tggaggaccg tgacccgctg ctccgccacg gcaccggcca gataggcgct gtcctgatgc
     5641 ccgcccggcc gggcgagcac cagctccgcg ccagccagga gaggacagaa gatctcccag
     5701 atggaggcgt cgaagccgta gggtgtcttc tgcagcaccc ggtcgtctgg cgccagggga
     5761 agctcgcact gcatccacag cagccggttg acgatcgccc ggtgggacac catcaccccc
     5821 ttggggcggc cggtggagcc cgacgtgtag atcacgtacg ccaggcggtc gtctacctcc
     5881 tctcccgctg gtggggaggg gatgagagag gcggccaggg ggtgaagggc ctcctccagc
     5941 aggaccacca tacccgcatg cgacggcaga gatccggcca ggcgctcctg ggtgagaact
     6001 gccgccggct ccacgtcctg gaggatgccc gccaagcgct cggcggggtg gtccgggtcc
     6061 aagggcaccc aggcgccgcc ggcctcgagg atcgccagga gcgccactac catctccggc
     6121 gagcgctcgg cgtggaccgc gaccggcacc tcgggcccca cccccagggc ccgcaaccgg
     6181 ctggcgagcc gcccggtccg ctccaccagc tcggcgaagg tcagggtccc ggactccgaa
     6241 cgaattgcca cctttccggg agtgcgcgcc gcctgctccc gcaccagctc gtggagcagc
     6301 cggcgcggca gctccatccg ggtggagttg tgctccacca gctcgcgccg ccgctccctt
     6361 ccggtcagca gctccaactc gccggcggcc gactccgggg ccgcggcggc ttggcgggcg
     6421 aggacggtga gccattcggc gatgtggtgg acgtccgtct ccaggaagcg ggaggcgtcg
     6481 tagtggaggg tcgcgcgcaa ggagccgccg ggctccacgg tacaggagag cttcaaggcg
     6541 aaccgctcgg tcatggcgct ggcccgctgc agcgtccaat gcagtccgcc ggctcctccg
     6601 agcgggagag gggactccac ctcgaacgcc agggaaggag ccaagccccc ctcgggagcc
     6661 aagcgatccc aggtgaaggc gttctgatgg gcccgggcct gctccagggc gcggccggct
     6721 cggcgggcga tctgcgcgaa gcgctccccc tcctcccagc ggaggaccac cggcaggaac
     6781 ttctccaacg gtcccacgac cgccgccagc tcggcgaagc accgtccctc ggaccgggcg
     6841 cacaccacca gctcgctccg gccggtcagg cgtccgatca ggacctgcca gcaggccagc
     6901 aggaacgcct ccggggccgc cctcgccaga cccgggccca ggtccaggcc ggctgcggcc
     6961 gtgcgaggcg cgaagccctc ctcggcggcg cgttcgaagg gcagcggctc ggcgagcagt
     7021 gatgccacct cctgctgccg ccagaaatcg cgctccgcct ccgtctcctc ggcatccagc
     7081 acctcgttct gccaggccgc cacgtcggcg tactgcatga ggtcgcccgc cggttcggcg
     7141 ctcccggcag agccataggc cgtggcgatc tcctccacca gattggccag cgaccgggcg
     7201 tcagcgcaga gcgcgggcag ggcgaggatc agccggtggc gttgcgggcc gtcggggatc
     7261 agcacggccc gcaacggcgg ctcctccccc aggctccacg gcctctccaa agccgccatc
     7321 gccgcccccc cctctcccca ggcgaacctc cgctcctcgc ccacgatctg gagaggaacg
     7381 gtcatccccg ggaggcagcg gaaggtggtg cggagggatt cgtggtgctc caccacccgc
     7441 tccaaggccg cccgcagccg ccgccggtcc agcgcccctt ccagcaggac ggcgcagctc
     7501 gcgcgatagg gcgcgccgtc ccggtcaccc ctcaggctcc acagccgccg ctgctggggc
     7561 gacaggggga acccctggag cgccacctgc tccagagtgc cgctcaccgc acggcctcct
     7621 cggcgaaggg cactccgacg ggggccgtcc tgcggctcct gcgcagccgg tcctggagcc
     7681 cctggcgctt tccctcctgg gcggccagcc gctgggccgc gtcggactcc tccagcgcct
     7741 cggccatctc ggcgaggcgt agctccggcc ggccggctac agcacgcagc agggcttcga
     7801 agttgccgag catgcggtcg atcgtagcgg cgtcgaagag gtccgtggcg tgctcgaccc
     7861 aggcgaggag gccctgctcg gagtcggcga ggttgaggat ccagtcgaac tgcgccgtct
     7921 ctcgtaccgg ccgctgcggc tcgatgcgga ggccggggag ctccagcgcc gccggcggca
     7981 ggttgagcaa gttgaccttg acctggaaca ccggcgagcg gcccaggctc cgctcgggat
     8041 tgagggcttc caccagcttg tcgaagggga gctcctgatg ggcataggcg cccagcatcg
     8101 tctcccgcac ccgggcgagc agctcgcgga acgtgggatc gccgcccagc cgggtgcgca
     8161 gcgctacctg gttgatgaag aagccgatga gtccctcggt ctccggccgg tcgcgccccg
     8221 ccacgtcggt gcccaccacc acgtcgtcct ggccggcgct ccagcccagg aggacctggg
     8281 ccgcggccag cagggccatg aacagggtca ccccctcgta ccccgcgagc tcgcggatcg
     8341 ctgccgccac ctcgggcgac agcaggcgcg agcgggtggc gcctcggaag ctctgcaccg
     8401 ccggacgcgg ccggtcggtg ggcagctcca gggcgggcgg cgcgccggcg agctgccgcc
     8461 gccagtagcc tacgtgggcg tcgagaacgg cgccgcgcag ccactcccgc tgccagcgag
     8521 catagtcggc gtactgcagg ggcagggctg ggagatccgg ccggcggccc gccagcaggg
     8581 cgccgtagac ctccctcacc tcctgcgtca gcacctggct ggaccagccg tcggccacga
     8641 tgtggtgcat ggtgaggagc agcagatgct cctctgccga caggcgcacc agagtggccc
     8701 gcagcagggg accgcgggcg aggccgaagg ggcgcagagc ctcctcggcg tcgaggcgcc
     8761 gggcctcgac ttgccgtgcc gtctcgggca gggcgaggag gtccgccagg gggagcgcca
     8821 ccggcgcggg cggagccacg gactggaccg gctgttcatc tacctcgggg aaggtggtgc
     8881 gcagcacttc atggcgccgc gccacctcgg agaagctacg ggcgagcgcc cccgggtcca
     8941 atgcgccggt gagccgcagc gtaaccggga cgttgtagaa cgggttgccc ggcgccagcc
     9001 ggtccaggaa ccagaggcgc tgctgggcga aggagaggag caaggggtga gtgccccggg
     9061 gcgaccgctc catcgggggc gccgcgaagc ccgggccgcc gcgtagggcg gcgtcgatcc
     9121 gctccgccag cgcggcgacg gtggggtgct cgaacaggct gcgcagcggc acgtccaccc
     9181 cgaaggcgcg gcgcagccgg gtgacgagtt gggtcgccag cagggagtgc cagcccaggg
     9241 tgaagacgtt gtcctgccgg cccaggcgct cgacgcccag gacctcggcc cagaggacgg
     9301 ccagcacctc ttcggtgggg gtacggaggg ccgcggagct ggcttcggag caggtggatg
     9361 gcgccggcag ggcggcgcgg tccaccttac cgctagcgag gcgcggcagg gccggcatgg
     9421 tgacgaaggc cgacggcacc atagcgtcag gcagccgctc ggccaggaag gctcgcaggt
     9481 ccgtggcggc cggcgcggcg gcccggagta cgacccaagc gatcaggcgc cggtcctccg
     9541 gtcccgcgcc gccatcgcgc accccggcca cggcctcagc cactccggga tgccggacca
     9601 gccagctctc cacctccccc agctcgatgc ggacgccacg gactttgacc tggtcgtcgg
     9661 cgcggccggc gtactccagg gctccggcca gagcctggcc gggggggacg tggcgcgtca
     9721 ggtcgccggt acggtagagg cggccccctg gctcaacggc gaacgggtcg ggcaggaagc
     9781 gctcggcggt gagatccggc cggtccagat agccgcgggc gagcccggtg ccggcgacgt
     9841 agagctcccc cacctctccc gcggggaccg gacggagaga ggcgtccagc agataggcgc
     9901 ggtcgttggc gatgggacgg ccgatgggga cggaggggtc gtccgggcgg caccgccaga
     9961 aggtggcgtc gatggcgcac tcagtcgggc cgtaaagg
//
LOCUS       DASPJI010000114        13778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_808546, whole genome shotgun sequence.
ACCESSION   DASPJI010000114 DASPJI010000000
VERSION     DASPJI010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13778)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13778
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808546"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VEL74_03075"
     CDS             <1..279
                     /locus_tag="VEL74_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61539.1"
                     /translation="RAATVNVPRSPHRQAPVPARRRAYRRPQHELPAEAVDRFAEGRA
                     SREERSAVLRHLLTGCAHCQRRLGAFWSCPAPVDTDAYDAMFDRLVAG"
     gene            426..1436
                     /locus_tag="VEL74_03080"
     CDS             426..1436
                     /locus_tag="VEL74_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYG61540.1"
                     /translation="MATRGPLAPHTCSLHSIRTVLIGCSLDEDSDDVVRAGLAVARAA
                     GARAFLVHAADFEPPLPVLEIAATAPAFYDEQATWRERQMADQVQRLGIADTELLGTR
                     VVVGPPHRVLADAARETGAELMVIGATRGRGVGNKLLGSTADRVLRKALCPVLVVRGE
                     LRMPPHRVLVPVDLSTLSGDAFHCGLHFLAEIAGDSPVEVAAFHAVTFLDLTAYRHRA
                     VPPVPETTEEVLRLVEGEMARFLHDSKVDGEAVESRVVAGDPRVEVLQELERRPADLV
                     IMGTHGRGGFDRFLLGSVSSSLAREAPCSLLLVPPEAALAEALADAVVARTTPDFGAV
                     PS"
     gene            complement(1468..4194)
                     /locus_tag="VEL74_03085"
     CDS             complement(1468..4194)
                     /locus_tag="VEL74_03085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG61541.1"
                     /translation="MLSVRRLVLPLFLALPLLAQDVPPPAADVPAGAVSAPVEVPVEV
                     WAQKGDLRRELTAADFAVTEGGEPRRVVDVRPLTGEPWRFVVYIDRVLSGTRSVRAAT
                     GALAERIRPLVGLGTVELVVAEPEPRVVVEATRHVAVLDEALSRLFLLGEGRDDVRQA
                     RQRFRERLRSLPEGEDAVELTGDSVEDEARMVRRQQERLAAWLAGRERGGPSVLFLVS
                     DGFDVDPRGFYLAELPKAEAPPRLADILAPHVLERDAAETARLTAALGWTVYPLPLGD
                     ESLPDLRRWRPRSSAEVPLGVSVTLPGQRQGAADGKPEPPPLPALLAPGEPLRWIAEA
                     SGGQVLTSRAEVAEAVARLRSRWLVAYETARPESGPSVPVEVAILDPKLASRSRRWEG
                     PVHLEEVAALRAREALAGEEMPGSLQVSAQVRLAPVEGDPAARQGLLEVRLDQGSLPP
                     DSRWRLSLALPAGSGAEVSHRGLSTADLAADPEGWTWRGTVPLTIDAERLAVVVEDLG
                     SGEWGGDVAALREEEEEAVGEAEVLPAPPARPAGAELRGFGVRILDPAPGRRVGPVDV
                     SAEVRVPEGRRVERVEFFWNDELAGTVYGPPFRHRVRVPLDRPEGYVRVSALLDDGST
                     TEDAVPLNGANVGERLDVRLRELYVVVTDRTGKPVRGLPRDRFRVRQDGTVQEIATFE
                     DAGSLPLTVALAIDSSASMFRKLENVQRAAASLLRGGLSTRDSALLVDFDTRPRLVTR
                     PTTDLPSVARSLDLLRPDGSTGLWEAVAFSLEQLGGVPGRKALIVYSDGVGEGEPFSY
                     RTTLRLARRSGVPVYLIVTNAESVHRSGGSLRPYAAKLRDLARASGGSVYFMQAEDDL
                     TGVYHEILAELRSQYTLAFYPKEEAGGWKEVRVEVQGEGLSARTVSGYEGEE"
     gene            complement(4242..4541)
                     /locus_tag="VEL74_03090"
     CDS             complement(4242..4541)
                     /locus_tag="VEL74_03090"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HYG61542.1"
                     /translation="MTSETRASPSLQTSFSSSECFSAPHEPIAAVDRCARAHPGAGTC
                     GLCFPQTERESPVYQHLDTGRRVSVPVHTGDVPKGTLRQILRDADLTVERLLELL"
     gene            complement(4465..4713)
                     /locus_tag="VEL74_03095"
     CDS             complement(4465..4713)
                     /locus_tag="VEL74_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HYG61543.1"
                     /translation="MLDSDGLMDKVEGYTVVYRQAEDVILAEVPAIPGCFSDGQSLEE
                     ARERVRDAILNFLDDLRDAGEPIPPDVLLVERVLLGAA"
     gene            complement(4717..6030)
                     /locus_tag="VEL74_03100"
     CDS             complement(4717..6030)
                     /locus_tag="VEL74_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HYG61544.1"
                     /translation="MKEIHELSPADLCALLDREGIRRFYLVWIPEERRIFASHPLVEP
                     LARLLEKDQRDFDRHEGIFMQVAPETGVLQGAFIHRTCRGQGAGGVRFWRYGTVEEYM
                     RDGLRLSKGMTLKNALAGLWWGGGKGVIARGTGASEDPAARRRVYEEYGDLLTALQGC
                     YVTAEDVGTSVEDMTAVYSRTRFTTCIPPAMGGSGNPSAPTARGVMRGMEAALAFLGR
                     GRLDGKTIAIQGLGNVGEPLIGYLRERGAARVIGSDIDPSRREALLAKFPPKEGWELE
                     VRTVERGDLSILAEPADILAPCATGGVLNPRTIPTIAAPIVCGAANNQLEDPERDDLL
                     LAERSITYLPDFLVNRMGIVNCADEQYGYVEPDPRVEAHLGTQWENSVYNLSIQVLEE
                     ARRTGKTPARIALALAEQRSFEMHPVWGHRGEQIIRSLVRNGWAG"
     gene            6122..6934
                     /locus_tag="VEL74_03105"
     CDS             6122..6934
                     /locus_tag="VEL74_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HYG61545.1"
                     /translation="MKRTCGALCAALLLAAPSSALAWGAVGHRVVGRIAQNHLAPEAA
                     RAIGCLMGTESLARSGTWADEIRSDPAWKRADPWHYINIEDRQTLETAERVAEGDILE
                     AMERFERVLRDPQAERQAKVEALRFLVHFVGDVHQPLHVGRKEDQGGNRVQVRWHGRE
                     TNLHTVWDSSMIDEERLSYTEWVEFIDHPTQAEIRTWQGTTYRDWIQESFAARAQVYD
                     IGDGNLSYKYSYRNLPLVRQRLLQAGVRLAGLLNSIFEQPAQAAQPATPPPA"
     gene            complement(6938..7171)
                     /locus_tag="VEL74_03110"
     CDS             complement(6938..7171)
                     /locus_tag="VEL74_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61546.1"
                     /translation="MKKKQKLVTVHVTTAPAPGEPSIVPVGGETLEALLEQWRVLQIQ
                     PLGQRTGDTYTALLLLEEAPSEPSLGRLGFGHE"
     gene            7280..7630
                     /locus_tag="VEL74_03115"
     CDS             7280..7630
                     /locus_tag="VEL74_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61547.1"
                     /translation="MKKLCLLGLALCMAAMPLAAAKSNTVTLVNKSDWDIHHLFLSSS
                     DDEEWGPDQLGDDVIEAGGSFKLTGIPCGNYDVKLVDEDGDECEVAEVDVCSGGETWR
                     ITNDDLLECEGYGE"
     gene            complement(7680..9155)
                     /gene="creD"
                     /locus_tag="VEL74_03120"
     CDS             complement(7680..9155)
                     /gene="creD"
                     /locus_tag="VEL74_03120"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HYG61548.1"
                     /translation="MKFVLDGSTAPGAPPRRPFRESVIWKLFVTGLLILVLMIPLAMV
                     RSLIGERQARRDEAVRELAATWGTEQTLGGPLLTVPFQVRSKDDKGKETITVTSAHFL
                     PESLRIEGRVVPERRSRGIFEVVLYRADLRVAGTFARPDLSDWRVAPEDVLWQDAFLT
                     VGVPDMRGIRSSVPIRWGNRELQLAPGGAESGVWASGLRVPIPDLAAGQAKDIYAFGF
                     DLGINGSGRLMFLPFGKETTVALTSTWADPSFVGAFLPESRKVNGTGFQSKWSVSYFG
                     RPYPQRWSAEEAQRLAALQVRADETRYTSSGVEYVPATPQSTLADTAFGVELLLPVDV
                     YQKSERSVKYAVLFLLLTFVTFFLYEVFSPFSLHPMQYLLVGSALCLFYLLLLSLAEH
                     APFGFAYLVASVATVLLIGGYSAAILRGKLRAFLMGVILGLLYGYLYILLQAEDYALL
                     LGAIGLFLILALIMYVTRRIDWSAPRTGKDGAAAQPVEVGA"
     gene            9898..12813
                     /locus_tag="VEL74_03125"
     CDS             9898..12813
                     /locus_tag="VEL74_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG61549.1"
                     /translation="MSRSKIFISYSHKDKLWLERLVEHLGVAPDEVALDLWDDRRIAM
                     GDEWLAEITRAIDQAHAAILLVSPSFLVSKFIRGVEVPRLLERRSKDGLRIFPVLIRS
                     CDWQVTPWLSSLQIRPDDAKPLDLLSKARRDFFLAAISREVRRILGQPSPPSRPVQPV
                     PINQPLLMLLHQISAPPADFTGREAELLDLHRLVEQGGVVIVGMGGLGKTTLAEKLAE
                     ELRPEYPDAQIYLDLKGVAPTPLSATEAMAHVVRTFHPEATVPAGEAELRSLYRSVLD
                     GKRALLLMDNAAGREQVEPLLPPRCCLLLVTSRFHFHVPGLVLKDLDELPEKDACDLL
                     LHIAPRLGDHAAEIARLCGRLPIALRLAGGALAERRDLSPAEYVRRLKDAQKRLRLID
                     SHLSLSYDLLSEDLQRRWRALGVFPGTFDAAAAAAVWGVEADPADEVLGILVKSSLVD
                     GEDGRYRLHDLARVFAESRLDEVEGTEARYRHAVYYGRVLNAADDLYLEGGESVLVGL
                     RLYDLEWGNIQAGFAWSAEGAEADEAAAKLCSGYLTAGECCLGLRQPKREQIHWLDSA
                     LAAARRLEDRSAEGRHLGKLGLAYAALGDVRRAIEHYKQHLRIAREIGDRPGEGQALG
                     SLGLAYAAFGDARRAIEHYEQWHQITREIGNRRGEGQALGNLGLAYAALGDACRAIGY
                     YEQVLQITREIGDRRGESNALGNLGLAYAALGDARRAIGYYEQDLQIAREIGDRRGEG
                     QALGNLGNSYAALGDARRAIGYYEQTLQIAREVGDRRSEGQTLGNLGNAYLAFGDAHR
                     AMEHYDQHLQTSREIGDRRGEGQALGNLGLAYAALGDAHRAIEFHEQRLQIARDIGDR
                     RGEGQALGSLGLAYAYLGDAHRAIEHYEQDLQIAREIGDRAGEARASWNLGLWYEAEG
                     DLRLAAELMQCWIEYVREIGHQEAETWAAEVEAIRARIEEEKSRPKKAARKRRGTSK"
     gene            complement(12870..13253)
                     /locus_tag="VEL74_03130"
     CDS             complement(12870..13253)
                     /locus_tag="VEL74_03130"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HYG61550.1"
                     /translation="MSKPDRIVIDDQVASGQPVIRRTRVPVSLVVGKLAGGMSFEELM
                     EEYALERQDILAALSYTARLLQDDRVVAILVEQGRLSSGKAAKLAGKSRVEFLSSLGK
                     SEVFTLESELRDLEAERISREAATE"
     gene            complement(13453..>13778)
                     /locus_tag="VEL74_03135"
     CDS             complement(13453..>13778)
                     /locus_tag="VEL74_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61551.1"
                     /translation="GDAPSGDFLLQQSAQLAAAVYPGTGKSKRYRQGKDHDGRGYPVY
                     YDGELAGHLRVFDEDLIAAMNVADALISAPYDFAHLFDAAGGLALERTGKLSLTRARG
                     EGGSV"
BASE COUNT         2158 a         4887 c         4695 g         2038 t
ORIGIN      
        1 cgagccgcta ccgtgaacgt tccgaggagc ccgcaccgcc aggctcccgt gccggcgcgc
       61 cgccgcgcct atcgccgtcc gcagcacgag ctgccggccg aagcggttga ccgcttcgcc
      121 gaggggaggg ccagccgcga ggagagaagc gcggtgctgc gccatctcct caccggctgc
      181 gcccattgcc agcggcgcct cggcgccttc tggtcctgcc ccgcgccggt ggacaccgac
      241 gcctatgacg cgatgttcga ccgcctcgtg gcaggatgag cctttaatct cgaagcgtcg
      301 aaaagacgct caccccgcca tttcggctgc ccccctggcg gccgatgttc ttcctacccg
      361 ggtggatggg acgcccgccg caaaaatacg gtagattcac ctcagaccga ttgcggagga
      421 atgccatggc cacacgggga cccctcgcgc cgcacacctg ttcgctccac tccatccgga
      481 ccgtgctcat cggctgctcg ctcgacgagg acagcgacga cgtcgtccgc gccggcctcg
      541 ccgtggcccg cgcggccgga gcccgggcct tcctggtcca tgccgccgac ttcgagccgc
      601 cgctccccgt cctggagatc gcggccacgg ctccggcctt ctacgacgag caggccacct
      661 ggcgggagcg ccagatggcc gatcaggtgc agcggctggg catcgccgac accgagctcc
      721 tggggacccg ggtcgtggtg ggccctcctc atcgcgtcct cgccgacgca gcgcgggaga
      781 ccggcgccga gctcatggtg atcggcgcca ctcgcggccg gggcgtgggc aacaagctcc
      841 tgggctcgac ggcggaccgc gtgctgcgca aggccctctg ccccgtgctg gtggtccggg
      901 gggagctgcg aatgccgcct caccgcgtgc tggtcccggt ggacctgtcg acgctctcgg
      961 gcgacgcctt ccactgcggc ctccacttcc ttgccgagat cgcgggcgac agcccggtcg
     1021 aagtagcggc gttccacgcg gtgaccttcc tggatctcac ggcctatcgt caccgcgccg
     1081 tgccgccggt gcccgagacc acggaggagg tgctgcgcct ggtggagggc gagatggctc
     1141 gcttcctcca cgacagcaag gtggacggcg aagcggtcga gagccgggtg gtggccggag
     1201 acccgcgggt ggaggttctg caagagctgg agaggcggcc ggcggacctg gtgatcatgg
     1261 gcacccatgg ccgcggaggc ttcgaccgct tcctgctcgg cagcgtctcg tcgtcgctgg
     1321 cgcgggaggc cccgtgcagc ctcctcctgg tgccccccga ggccgccctg gcggaagccc
     1381 tggccgacgc cgtcgtggcc cgcaccaccc cggatttcgg agccgttcca tcctgaagcg
     1441 gaccggctcc aggctgaaag gggagcccta ctcctccccc tcgtacccgc tgaccgtgcg
     1501 ggcgctcaag ccctcgccct ggacctcgac ccgcacctct ttccagcccc cggcctcttc
     1561 cttcggatag aaggccaggg tgtactggct gcgcaactcg gccaggatct cgtgatagac
     1621 gccggtcagg tcgtcctcgg cctgcatgaa gtagacgctg cctcccgagg ccctggccag
     1681 gtcgcggagc ttggccgcgt aggggcggag cgatccgccg gagcggtgca cggactcggc
     1741 gttggtgacg atcaggtaga ccggaacgcc gctccggcgc gccagccgca gggtcgtccg
     1801 gtaggagaag ggctccccct cccccacgcc gtccgagtag acgatcagcg ccttgcggcc
     1861 cgggactccc ccgagttgct ccaaggagaa ggccacggcc tcccacagcc ccgtgctgcc
     1921 gtccggccgc aggaggtcca gcgagcgggc cacggagggc aggtcggtgg tcgggcgggt
     1981 gaccaggcgc gggcgggtgt cgaaatccac cagcagcgcg ctgtcccggg tcgagagccc
     2041 gccgcgaagc agggacgccg ccgcccgctg cacgttctcc agcttccgga acatgctggc
     2101 cgaggagtcg atggccagcg ccacggtcag cggcaggctg cccgcgtcct cgaaggtggc
     2161 gatctcctgg acggtgccgt cctggcgcac ccggaagcgg tcgcgtggca gcccccgcac
     2221 cggcttgccc gtccggtcgg tgaccaccac gtacagctcc cgcagccgga cgtccagccg
     2281 ctcgccgacg ttggcgccgt tgaggggcac ggcgtcctcg gtcgtcgagc cgtcgtcgag
     2341 gagggcggag acccgcacgt agccctccgg ccggtccagc gggacccgga cgcggtgccg
     2401 gaacggcggc ccatagacgg tgcccgccag ctcgtcgttc cagaagaact cgacccgctc
     2461 gacgcgccgc ccctcgggca cccggacctc ggccgacacg tccaccggac ccacccggcg
     2521 gcccggcgcc gggtccagga tgcgcacgcc gaagccgcgc agctccgcgc cggcggggcg
     2581 cgccggcggc gccgggagca cctcggcctc cccgaccgcc tcctcttcct cttctctcag
     2641 ggcggccacg tccccacccc attcgccgct cccgaggtcc tccaccacta cggccagccg
     2701 ctcggcgtcg atggtcagcg gcaccgtgcc gcgccaggtc cagccttcgg gatcggcggc
     2761 gaggtccgcc gtggacagcc cgcggtggga gacctcggcg ccgctgccgg ccggaagcgc
     2821 gaggctcagg cgccagcggc tgtcgggggg cagggagccc tggtcgagcc ggacctcgag
     2881 gagcccctgc cgcgcggcgg ggtccccctc cacgggcgcg aggcggacct gcgcgctcac
     2941 ctggaggctc cccggcatct cctcgccggc cagggcctcg cgggcgcgga gcgccgccac
     3001 ctcttccaga tggacggggc cttcccaccg ccgcgaccgg ctggccagct tcggatcgag
     3061 gatcgccacc tccacgggga cgctggggcc agactcgggc cgcgccgtct cgtaggcgac
     3121 cagccagcgg ctgcgcaggc gggcgacggc ctcggccacc tcggcgcggc tggtcagcac
     3181 ctgtccgccg ctggcctcgg cgatccagcg cagaggctcc ccgggagcga ggagcgcggg
     3241 cagcggcggc ggctccggct tcccgtccgc cgccccttgc cgctggccgg gcagggtcac
     3301 cgacaccccg agcggcacct cggccgagct cctgggccgc cagcgccgga ggtccgggag
     3361 gctctcgtcc ccgaggggca gcggatagac cgtccagccc agggcggccg tcaggcgcgc
     3421 ggtctccgcg gcgtcccgct ccaggacgtg gggggcgagg atatccgcca gccggggcgg
     3481 cgcctcggcc ttcgggagct cggcgaggta gaagccccgg ggatcgacat cgaagccgtc
     3541 gctcaccagg aagaggaccg agggcccgcc gcgctcccgg ccggcgagcc aggcagccag
     3601 ccgctcctgc tggcgccgca ccatgcgcgc ctcgtcctcc accgagtcgc cggtgagctc
     3661 gacggcgtcc tcgccttcgg ggagactgcg tagccgttca cggaagcgct gccgcgcctg
     3721 gcgcacgtcg tcccgcccct cccccaggag gaagagcctc gacagcgcct cgtcgagcac
     3781 cgcgacgtgg cgggtcgcct cgaccaccac ccgcggctcg ggctcggcga cgaccagctc
     3841 gaccgtgccc agccccacca gcgggcggat ccgctccgcc agggcgccgg tggcggcgcg
     3901 caccgagcgt gtgcccgaga gcacccggtc gatgtagacg acgaaccgcc acggctcgcc
     3961 ggtgagcggc cggacgtcga cgacccggcg cggctcgccc ccctcggtga cggcgaagtc
     4021 ggcggcggtc agctcgcgcc ggagatcccc cttctgggcc cagacctcga ccgggacctc
     4081 gacgggggcg gatacggcac cggccgggac atccgccgcc ggagggggga cgtcctgagc
     4141 cagcaggggg agagcgagga acaacggcag aacgagacgg cgtacggaaa gcacggcccc
     4201 attctaaggg gggagagggg taccgggaga gatccgagtg attacaggag ctcgagaaga
     4261 cgctcgaccg tcaggtcagc atcgcgcagg atctggcgaa gggtcccctt cggcacgtct
     4321 ccggtatgga ccggcacgga gacccgccgc cctgtgtcca gatgctgata gaccggtgac
     4381 tccctctctg tctgcggaaa gcaaagcccg cacgttccag cgcccgggtg agctctcgca
     4441 caccgatcga cggcagcgat gggctcatgc ggcgccgaga agcactcgct cgacgagaag
     4501 gacgtctgga gggatgggct cgcccgcgtc tctgaggtca tccaggaagt tcaggatggc
     4561 atcccggacc cgctcgcgag cctcttccag gctctggcca tccgagaagc atccagggat
     4621 ggcgggcacc tcggcgagga tcacatcctc tgcctggcga tagacgacgg tatacccttc
     4681 gaccttatcc ataagcccat cagagtctag caccccctac cccgcccagc cgttgcgcac
     4741 cagcgagcgg atgatctgct ctccgcggtg cccccagacg gggtgcatct cgaaggagcg
     4801 ctgctcggcc agcgccaggg cgatgcgggc cggggtcttg cccgtgcgcc gcgcctcctc
     4861 cagcacctgg atcgacaggt tgtagacgct gttctcccac tgggtgccca ggtgcgcctc
     4921 gacccgcggg tcgggctcca cgtagccgta ctgctcgtcg gcgcagttga cgatccccat
     4981 gcggttcacc agaaagtcgg gcagataggt gatgctccgc tcggcgagga gcaggtcgtc
     5041 ccgctcgggg tcctccaact ggttgttggc ggcgccgcag acgatggggg cggcgatcgt
     5101 ggggatggtc cgcgggttca gcaccccgcc ggtggcgcag ggggcgagga tgtcggccgg
     5161 ctcggcgagg atcgagaggt ccccccgctc gacggtccgc acctccagct cccacccctc
     5221 cttgggaggg aacttcgcca gcagcgcctc gcggcgggag ggatcgatat ccgacccgat
     5281 gacccgcgcc gccccgcgct cccgcaggta gccgatgagc ggctcgccga cgttccccag
     5341 gccctggatg gcgatcgtct tgccgtccag ccgcccccgg cccaggaacg ccagggccgc
     5401 ctccatcccc cgcatcaccc cgcgcgccgt gggagccgaa gggttgccgc tgccgcccat
     5461 cgccggcggg atgcaggtag tgaagcgggt gcgcgaatag accgccgtca tgtcctccac
     5521 cgaggtgccc acgtcctcgg cggtgacgta gcagccctgg agcgcggtga ggaggtcgcc
     5581 atactcctcg tagacccgcc gccgcgccgc ggggtcctcg gaggcgccgg tgccgcgggc
     5641 gatcaccccc ttgccgccgc cccaccagag gccggcgagg gcgttcttga gcgtcatgcc
     5701 cttggacagc cgcaagccgt cgcgcatgta ctcctccacc gtgccgtagc gccagaagcg
     5761 gacgccgcca gccccctgcc cgcggcaggt gcggtggatg aaggccccct gcaacacccc
     5821 ggtctccggg gccacctgca tgaagatccc ctcgtggcgg tcgaagtcgc gctggtcctt
     5881 ctccaggagg cgcgccagcg gctcgaccag cgggtgcgaa gcgaagatcc gcctctcctc
     5941 ggggatccag accagataga agcggcggat gccctcgcgg tcgagaagag cgcagaggtc
     6001 ggccggggac agctcatgga tttccttcat ctcatcgatc tcctggggcg cgccgcatcc
     6061 taccaccagc cacctggcgt ggcgctgcta agatcggccc ggtcatgaaa ggagcccgag
     6121 cgtgaaacgg acctgtggag ccctctgcgc ggcgctgctg ctggcggccc cctcctccgc
     6181 cctggcctgg ggggccgtcg gccaccgggt cgtggggcgg atcgcccaga accacctggc
     6241 gccggaggcg gcgcgggcca tcgggtgcct gatgggaacc gagagcctcg cccggtccgg
     6301 cacctgggcg gacgagatcc gctccgaccc ggcctggaag cgggccgatc cctggcatta
     6361 catcaacatc gaggatcgcc agaccctgga gaccgccgag cgggtcgccg agggggacat
     6421 cctggaggcc atggagcgct tcgaaagggt gctgcgcgac ccccaggccg aacgccaggc
     6481 caaggtggag gccctgcgct tcctcgtcca cttcgtcggc gacgtccacc agccgctgca
     6541 cgtcgggcgc aaggaggacc aggggggcaa cagggtccag gtgcggtggc acgggaggga
     6601 gaccaacctg cacaccgtct gggactcctc gatgatcgac gaggaaaggc tcagctacac
     6661 cgagtgggtg gagttcatcg accacccgac gcaggcggag atccggacct ggcagggcac
     6721 cacctatcgc gactggatcc aggagtcctt cgccgcccgg gcccaggtct acgacatcgg
     6781 cgacggcaat ctgagctaca agtactccta ccggaacctc cccctggtcc ggcagcggct
     6841 gctccaggcc ggggtgcgcc tggccgggct gctgaactcg atcttcgagc agcccgccca
     6901 ggctgcccag cctgcgacac cgccgcccgc gtagacccta ctcgtgtccg aagccgagcc
     6961 gaccgaggct cggctccgaa ggcgcctcct cgaggagcag gagggcggta taggtatccc
     7021 ccgtcctctg ccccagcggc tggatctgca ggacccgcca ctgctccagc agagcctcca
     7081 gggtttcgcc gccgacgggc acgatggacg gctcgccggg ggccggcgcg gtcgtgacgt
     7141 ggacggttac gagcttctgc tttttcttca acgtgctctc ctctttctcc cgtgctcggg
     7201 gcggcgccgc gaagtataga ccggcgccag accgtgctag gatctccgcc gcatcctctt
     7261 tccttcaagg agattttcga tgaagaagct gtgccttctg gggctcgctc tgtgcatggc
     7321 cgccatgccg ctggccgccg cgaagtcgaa caccgtgacc ctggtcaaca agtccgactg
     7381 ggacatccac cacctcttcc tctcctcctc cgacgacgag gagtggggtc ccgatcagct
     7441 cggcgacgat gtcatcgagg cgggcggctc gttcaagctg accggcatcc cctgcggcaa
     7501 ctacgacgtg aagctggtgg acgaggacgg cgacgagtgc gaggtggccg aggtggacgt
     7561 ctgcagcggc ggcgagacct ggcggatcac caacgacgat ctcctcgagt gcgaggggta
     7621 cggcgagtag agcccggcgg ggccggcggc gaccgccggc ccctctccca cacccttcct
     7681 caggccccca cctccaccgg ctgcgctgcc gctccgtcct tccccgtccg tggcgcggac
     7741 cagtcgatcc gccgcgtcac gtacatgatc agggcgagga tcaggaagag cccgatggcg
     7801 cccaggagca gcgcgtagtc ctcggcctgg agcaggatgt agagatagcc gtagaggagg
     7861 cccaggatca cccccatgag gaacgcccgc agcttcccgc gcaggatggc cgccgagtag
     7921 ccgccgatca gcagcaccgt ggccaccgag gccaccaggt aggcgaagcc gaacggcgcg
     7981 tgctccgcga gcgacaggag cagcaggtag aagaggcaga gcgccgagcc caccagcagg
     8041 tactgcatgg ggtgcagcga gaacgggctg aacacctcgt agaggaagaa ggtcacgaag
     8101 gtcagcagca ggaagagcac cgcgtacttc accgaccgct ccgacttctg gtagacgtcg
     8161 accggcagca gcagctcgac gccgaaggcg gtatcggcca gggtgctttg cggcgtggcg
     8221 gggacgtact cgactccgga ggaggtgtat cgggtctcgt cggcacgcac ttgcagcgcc
     8281 gccagccgct gcgcctcctc ggcgctccag cgctgcgggt agggacggcc gaagtaggag
     8341 acgctccact tcgactggaa cccggtcccg ttcaccttcc gggactcagg gaggaaggcc
     8401 cctacgaagc tggggtcggc ccaggtcgaa gtcaacgcga cggtggtctc cttgccgaac
     8461 ggcaggaaca tcagccggcc gctgccgttg atccccaggt cgaagccgaa ggcatagatg
     8521 tccttcgcct ggccggcggc gaggtccggg atcggcaccc gcagcccgga ggcccagacg
     8581 cccgactcgg cgccgcccgg cgcgagctgc agctcccggt tcccccaacg gatcggcacg
     8641 ctgctgcgga tgccccgcat gtctggcacg cccacggtga ggaaggcgtc ctgccagagg
     8701 acgtcctcgg gcgccacgcg ccagtcgctc aggtccggcc gcgcgaaggt gccggccacg
     8761 cgcagatcgg cccggtagag gaccacctcg aagatccccc ggctccgccg ctcgggcacc
     8821 accctgccct cgatccgcag cgactcgggt aggaagtggg ccgacgtcac cgtgatcgtc
     8881 tccttcccct tgtcatcctt ggaccgcacc tggaacggca cggtgaggag cggaccgccc
     8941 agggtctgct ccgtccccca ggtcgccgcc agctcgcgga cggcctcgtc gcggcgcgcc
     9001 tggcgctcgc cgatcagcga ccgcaccatg gccagcggga tcatcagcac caggatcaac
     9061 agtccggtca cgaacagctt ccagatcacc gactcccgga acggccgccg cgggggcgcg
     9121 cccggcgccg tggacccgtc gaggacgaat ttcatgactc ttccctccgt ctatgtctct
     9181 gtccagggag ccggaggcgt tccggtcccg gagaccattg gacgaagccg gcggggcgcg
     9241 gtgatggagg gattcaggca ggccgcgggc agattgtggc ggaaccatgg cgccctcacc
     9301 cccggatccc gcgctacctc gtgccgcagg gggaggtggt gcctcccccc ctgggcggac
     9361 gggcgggagg gagcggggga ggtctaggag gggggttttt gcagcgcccc gagggagatc
     9421 ctgtatcctt gctggcgcgc gccacgcctc ctacgtctcc accgccggag aatggctggc
     9481 gcggagggtc ctccccaaaa tgggaagccc gtctccccca aaagggaaga gcctctcccc
     9541 attgcgggag ggcaggctcc ccgaaaggga gagacccttc accgatccgg tacggccttc
     9601 tccctgaacg ggtatggccc tctcccgtat ccggtacatc cccttcccct ttcaggtaca
     9661 ccccttcccc aaaagggtag acccctctcc ctcaaaggta cgagcctcat cccagccttc
     9721 atcgtcgttg cctggcgtcc tccagccggc gcctgacagt ccatcacgtc aggctggcta
     9781 tccgcggcgc tcatttctct aggaaacacg gaggagtcgg gctggagaag gagcgaggcc
     9841 aggcggatga cggctccccc tccgcgtttg tccccccagc cgctagaatc ttcgaagatg
     9901 tcccggtcca agatcttcat cagctacagc cacaaggaca agctatggct ggagcggctc
     9961 gtcgagcacc tcggagtcgc tcccgacgag gtcgctctcg acctctggga cgaccggcgc
    10021 atcgccatgg gggacgagtg gctcgcggag atcacgcgag cgatcgacca agcccacgca
    10081 gcgatcctgc tggtctctcc ttccttcctg gtctccaagt tcattcgcgg cgtcgaagtg
    10141 ccccggctgc tggagcggcg gagcaaggat gggcttcgaa tcttccctgt cttgatccgc
    10201 tcgtgcgact ggcaggtcac cccctggctg tcctctttgc agatccggcc ggacgacgcc
    10261 aagcctctcg accttctcag caaggcacgg cgagatttct tcctggccgc gatcagccgg
    10321 gaggtccggc gcatcctcgg gcagccctcc cccccgtccc ggcccgttca gcccgttccg
    10381 atcaaccagc ctctcctcat gctcctccac cagatctccg ccccgcccgc cgacttcacc
    10441 ggccgcgagg cggagctgtt ggatctccac cggttggtcg agcagggtgg cgtcgtcatc
    10501 gtggggatgg gtgggctcgg caagaccacc ctcgccgaaa agctcgccga ggagttgaga
    10561 cccgagtacc cggacgctca gatctacctc gacctgaagg gcgtcgcccc aactcctctc
    10621 tccgctacgg aggccatggc ccacgtggtg cggacgttcc atccggaggc gacggtcccg
    10681 gctggtgagg cggagctgcg gtccctctat cgttcagtgc ttgatggcaa gcgggcgctt
    10741 ctcctgatgg acaacgccgc cggccgggag caggtcgagc cgctcctccc tccccggtgc
    10801 tgcctgctcc tcgtgacctc gcgcttccac ttccacgtcc ccggcctcgt gctcaaggat
    10861 ctggacgagt tgcccgagaa agacgcctgc gacctgctgc tgcacatcgc gcctcgcctc
    10921 ggagaccacg ctgccgagat cgctcggctc tgcggccggt tgcccattgc cctccgcctt
    10981 gccggtggtg ccttggccga gcggcgggac ctctcccccg ccgagtatgt ccgcaggctg
    11041 aaagacgctc agaaacgtct gaggcttatc gactcccacc tctctctgag ctacgacctg
    11101 ctgagcgagg atctccaaag gcgctggaga gcgctcgggg tcttcccggg aaccttcgat
    11161 gccgctgctg cggctgcggt ctggggagtg gaggccgatc ctgccgacga ggtgctcggg
    11221 atcctggtaa agagcagcct tgtagatggc gaggacggcc ggtaccggct ccacgatctg
    11281 gcccgggtct ttgcggagag ccggctggat gaggtggagg ggacggaggc tcggtacaga
    11341 catgcggtgt attacgggag agtccttaat gccgcggatg acctgtactt agaaggtggt
    11401 gagtctgttc tggtgggcct gcggctttac gacctggagt gggggaacat tcaggccgga
    11461 tttgcctggt cagcggaagg ggccgaggcg gatgaggcgg cagccaagct ctgtagtgga
    11521 tatctgactg ccggggagtg ctgcctgggg ctgcgccagc cgaagagaga gcaaatccat
    11581 tggctggaca gcgctcttgc cgctgctcgc cgcctcgaag accgctccgc ggaagggagg
    11641 cacctcggaa agctcggcct cgcctatgcg gcccttggcg atgtgcgtcg tgccatcgag
    11701 cactacaaac aacatctccg gatcgcccgc gagattggcg accggcctgg cgagggtcaa
    11761 gccctcggca gcctgggcct cgcctacgcg gcctttggtg atgcgcgccg cgccatcgag
    11821 cactacgagc agtggcacca gattacccgc gagattggca accgacgcgg cgagggccaa
    11881 gccctcggaa acctgggcct cgcctacgca gcccttggtg atgcgtgtcg cgccatcggg
    11941 tactacgagc aggttctcca gatcacccgt gagattggcg accggcgtgg cgagagcaac
    12001 gccctcggaa acctaggcct cgcctacgcg gcccttggcg atgcgcgtcg cgccatcggg
    12061 tactacgagc aggatctcca gatcgcccgc gagatcggcg accggcgcgg tgagggccaa
    12121 gcccttggaa acctgggcaa ctcctacgcg gccctcggcg atgcgcgtcg cgccatcggg
    12181 tactacgagc agactctcca gatcgcccgt gaagtcggcg atcggcgcag cgagggccaa
    12241 acccttggaa atctgggcaa tgcctacctg gcctttggcg atgcgcatcg cgcaatggag
    12301 cattatgacc agcatctcca gacctcccgc gagatcggcg accggcgtgg cgagggccaa
    12361 gccctcggca acctgggcct tgcctacgcg gcccttggcg acgcacatcg cgccattgaa
    12421 ttccacgagc agcgcctcca gatcgcccgt gatattggcg accggcgcgg tgagggacaa
    12481 gccctcggaa gcctaggcct cgcctatgcg taccttggcg atgcgcatcg cgccatcgag
    12541 cactacgagc aggatctcca gatcgcccgc gagatcggcg accgggcggg cgaagcgcgg
    12601 gccagttgga atctgggcct ttggtatgag gcagagggtg atctgaggct agccgcagag
    12661 ttgatgcagt gttggattga atacgtccgt gagatcggtc accaggaggc cgagacgtgg
    12721 gcagccgagg tggaagcgat ccgcgcccgg atcgaggaag aaaagagccg cccgaagaag
    12781 gcggctagaa agaggcgggg gacctccaag tagccgcgga gcggcgacag aatttagcct
    12841 ggcgcgtgag cgggctcacg cccggggctt tattctgtcg ccgcttcgcg gctgatccgc
    12901 tcggcttcca gatcgcgcag ctcggattcg agggtgaaga cctcgctctt tccaaggctc
    12961 gataggaact ccaccctgga tttccccgca agcttggcgg cctttccaga cgaaagccgc
    13021 ccctgctcca cgaggatcgc tacgacgcgg tcgtcctgaa ggaggcgggc ggtataggag
    13081 agagcggcca ggatgtcctg acgctcgagg gcgtactcct ccatgagctc ctcgaaggac
    13141 atccccccgg ccagtttgcc cacgacaaga gacaccggga cccgagtcct ccggatcacg
    13201 ggttggccag aggccacttg gtcgtcaata acgatccggt ccggcttgct catcggcgtg
    13261 atcgtacacc gggggcgggt gccgcgcggc gcggctgaaa aaaagaagaa ccccctcctg
    13321 acctcccccg ctccctccca cgcacccgcc caggggggag gaagcccgcc cccttgcagc
    13381 tagaaggtga gagcgccggg gcggccaaag ccgccccggc gaaggaagtc cttccttatc
    13441 tatatagggc gatcagaccg agcccccctc ccccctcgcg cgggtgaggg agagcttgcc
    13501 ggtgcgctcc agcgccaggc ctccggcggc gtcgaagagg tgggcgaagt cgtagggggc
    13561 gctgatcagg gcgtcggcga cgttcatcgc ggcgatcagg tcctcgtcga agacgcggag
    13621 gtgtccggcc agctcgccgt cgtagtagac gggatagccc cggccgtcgt ggtccttgcc
    13681 ctgacgatag cgcttgctct tgcccgtgcc ggggtagacg gcggcggcga gctgcgcgga
    13741 ctgctggagg aggaagtccc cggacggagc gtcacctc
//
LOCUS       DASPJI010000115        10032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_121126, whole genome shotgun sequence.
ACCESSION   DASPJI010000115 DASPJI010000000
VERSION     DASPJI010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10032)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10032
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121126"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..845)
                     /locus_tag="VEL74_03140"
                     /pseudo
     CDS             complement(1..845)
                     /locus_tag="VEL74_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874636.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            1035..1880
                     /locus_tag="VEL74_03145"
     CDS             1035..1880
                     /locus_tag="VEL74_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HYG61552.1"
                     /translation="MRINSCAAVLGILGLVFFGGNAEAATRTENFAKSYPLGAGGEVQ
                     LRNVNGGVTLEAWDRNEVRVEAEKKARGRDDAAIRKALQEIRIEVSRTAGGLRIETRL
                     PSRKNGFLDWLTGDDVSTSVEYRIRVPRNSPLGIQSVNGGLKLTGTRGRAHLTTTNGG
                     IDVAQVTGNLRLETINGGINVASSAGAVNASSTNGGIEVRLADVPDGSDLSFETINGG
                     ITLQLPGDVRATVDAATTNGQVSSDFQVSGDTKSRRRVAGEINGGGGTLRLRTSNGSI
                     HIVEG"
     gene            2093..2980
                     /locus_tag="VEL74_03150"
     CDS             2093..2980
                     /locus_tag="VEL74_03150"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HYG61553.1"
                     /translation="MTMNRQQWTLVSGLGMGAGLMYLLDPQAGGRRRALVRDKAVSAY
                     SSGSDVLRKTSRDLGNRSKGLAAGVRSKLKKTEEDADDLVLRDRVRSKMGRWVSKAGA
                     IDVTVQDCVVTLRGHVPEDEVDRLLKKVRKVKGVEDVVCELAIGDSEDASSLLSEGEV
                     QGSTRNWSKAARLLAGTAGGALAVAALTQRSKMAPALGATLGTVGLGLLAGGVGGSNV
                     NPKRLLKRRGHSQDQGQSLTDGESGQSFGDMSGDGLESKALADSGGDGSAVRHHDSHE
                     HGKSESKSDRFDYEPVHTH"
     gene            complement(3107..3277)
                     /locus_tag="VEL74_03155"
     CDS             complement(3107..3277)
                     /locus_tag="VEL74_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61554.1"
                     /translation="MKKKTKKLVLAKETVRKLGEGLLVKAAGGDTVSYCAAGCATGSC
                     RICLPDSINDIC"
     gene            3517..5079
                     /locus_tag="VEL74_03160"
     CDS             3517..5079
                     /locus_tag="VEL74_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYG61555.1"
                     /translation="MRRVGVFGWGIVAPKSPNIEAFAGNLERADSWLEPFNGFGPDNF
                     LVGRPDFSFEGYHDWIAARFPPSRFRQLQEKMDYPTLYTVGSFIQSLEQNPGLEEELR
                     RLGTAAHVYVGTGLGNVATIAEGALTLDRAQRRWDRFWAERNPAMREHLAAIAEAAPT
                     DPAVPPRPETAPPEDRDDAERAWWHYWAARSAELRQYLAELAEIEGLSVQGDVEHGKM
                     SVMKEKQRRTAQLQKRWQTPTPPWQSVSANLVWNISNTPAAQVSMLGHITGLAFAPVA
                     ACSTFGVCLKLGMDAIARGEAKAVVVGATDPPPHPMVVGAFFAARVISADADVSKPLT
                     GLRGTHVAGGAVVWIVGDYEYMTAKGFKPLGMEPMAVGVSSDADHIITPSAEGPTAAI
                     HQAFELSGIRPDEIASWDLHATATPGDYKEVSTMRGVVPASVLVTARKGTFGHGMAAG
                     GGWELTAQYLGYERGQLYPTPLHLEEVNRQIAGVHDLYVFDRPCDAPKGPAGKLSMGI
                     GGINACVISRPW"
     gene            5125..6126
                     /locus_tag="VEL74_03165"
     CDS             5125..6126
                     /locus_tag="VEL74_03165"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYG61556.1"
                     /translation="MKVLVTGGTGFLGRRIVSELAPRHSLRLLVRSTASRERFPAGVE
                     FATGDVTDRASLERAAAGCDAVIHAAALVKILAPPADFDRINVAGLDNVLHAAEQAGT
                     VEKVVYVSSFMALGPTEAGPGGMLDEKADLRDRSWINDYERTKAHSDRQARKAIADGA
                     PVVIVYPGVIYGPGEMTEGNIVVRHVLDLVHRRLPALLGSPARRWNYVYVDDVARGVA
                     LALEKGDPGSRYVLGGENIAQGDFYALVERLTGVKVPSLRMPDGIAKAAGAAQKTWAR
                     LRGTTPQLTPDLVEVYRHDWAFSSARAEAELGYRWRPLAEGLATTLTWLKETGQWKP"
     gene            6114..7502
                     /locus_tag="VEL74_03170"
     CDS             6114..7502
                     /locus_tag="VEL74_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HYG61557.1"
                     /translation="METVSAPPAPSSGVRRGLSGGELLRKVVHMSMGLFALSLRWLGP
                     FWGAVLALTALAFNLFLFPRLGGRRLWRQHEVSRGSSIGIVLYPLAVLILILVFYRRL
                     EVAAAAWGILAFGDGMASVVGMQFGRRKLPWNPGKSWLGSIAYVFFGGMAAAALLLWT
                     APGQYSPAFALTCGLVTALLAAGLESLPQGLDDNIGVPLLSGLFLLGLLLTQGRWDDF
                     WAQEGLATRLLLAAAVNAALAGLAYAAKTVDRSGLVVGFLVGFSIFAFAGWQGFLILL
                     TFFIIGSACTKIGYRRKAAAKLAQEKGGRRGARHALANAGVGMACALFLALTPHPVLF
                     GLALAGAFATAASDTASSEIGQLLGRRTFLLTTFRPVPRGTEGAVSVEGTLAGVIASL
                     VIGGLGALLGLYPWLGVAFVAVAAFVGTTFESLVGAALEKRNLLDNEALNFLNTLVGA
                     LVAAAFAPLVVP"
     gene            7499..8521
                     /locus_tag="VEL74_03175"
     CDS             7499..8521
                     /locus_tag="VEL74_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HYG61558.1"
                     /translation="MISQTERVGAPAPSKLSLYTQLARPFTLLPPLLGIVSGAVCAFG
                     SVHNPDPLNRLTLSVILTVLLGSLCASFMNAASNAINQIYDLDIDRLNKPNRPLVTGA
                     LSVREVWGFSWIFYALALIPTWLVVPYPYETFMEKLTAPVRYHETFFIYLVGLLFTFI
                     YSAPELGRTKAKGMLANFTIAIPRGCLLKVAGWAMVASVWATEPWYIGAVFAFFLIGA
                     ASTKDFADIEGDAAGGCKTLPILYGVEKAAWIISPFFVLPWLLLPLGAFLPDPQSPEH
                     PILTGNPWMLTGLGVLLTVWGIYTVYLLLRDPESLTKTENHPSWKHMYLMMMAAQVGF
                     ALAYLF"
     gene            8616..9317
                     /locus_tag="VEL74_03180"
     CDS             8616..9317
                     /locus_tag="VEL74_03180"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HYG61559.1"
                     /translation="MPDDYMTTDEPLKRPLADELSAAVAEDLETFDSAQAERDSDDAF
                     ASGSAGDRELPSTGLLSFYDRLRDRILRTAEKRGGKLSEGTLKALLLVPDVFILLVRL
                     TLDKNVPASTRAMIGGALAYFVLPADLLPEMLLGGVGFMDDLVLATAVLSQAFSGDLE
                     PYARKHWSGPDDLRVVLQDVTTTAQSLLGQNLYGKLRGLMSRRGIQVQEDDRYEEEED
                     GYDSGYGEAPPRPRR"
     gene            9462..9803
                     /locus_tag="VEL74_03185"
     CDS             9462..9803
                     /locus_tag="VEL74_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61560.1"
                     /translation="MKKALLWSVFAWMLCTGLAVAGEPVAPLPQTPQPAIAGESCHGQ
                     PVSPLVADAASTVPAPLDLFTPEPQQAAICPLIGCVDDQYCRRDRDCTAAPGGVCNLF
                     CPTRGCCSYTG"
BASE COUNT         1435 a         3593 c         3439 g         1565 t
ORIGIN      
        1 gccagcttat ccacctcgat gatctcgatg atcacatcga aggtcggcgg ggccttgcgc
       61 tccaggacgg tcttctgcgt gccgcgcttg cgcgcctcct aatcgccgag cgtcacgctc
      121 tggatgccgc cgaccaggtc cgagagcgtc gggttctgca gcaggttgtc gagcgtgttg
      181 ccgtgcgccg tggcgatgag ctggacgccg cgctcggcga tggtgcgcgc cgccgccgtc
      241 tccgcggtgg tgccgatctc gtcgatgacg atcacctgcg gcatgtggtt ctcgaccgcc
      301 tcgatcatca ccgcgtgctg cagctcgggg gcgggcacct gcatgcggcg ggcgcggccg
      361 atcccgggat gcgggatgtc gccgtcgccg gcgatctcgt tcgaggtgtc gaccacgatc
      421 acccgcttgt tgagctcgtc ggcgagcacc cgcgccgcct cgcgcagcag cgtcgtcttg
      481 ccgacgcccg ggcggccgag gagcaggatc gactggccgg cctcgatgac gtcgcgcagg
      541 atgtccaccg tgccgaacac cgcccggccg acccggcagg tgaggccgat gacgtcgccg
      601 acccggttgc ggatcgccga gatgcggtgc agggtgcgct cgatgccggc gcggttgtcc
      661 ttgccgaagc ggccgacccg ctcgacgaca taggagatgt cctcgcggat gaccggttcc
      721 gaggacagct cccgtccgct gtcggggagg cgggcctcgg gcagccgccc gaggtcgagg
      781 accacctcga ccaggctctc cacctcggcc atctcctcga tggcctggcg cagccgcggc
      841 ggcatgaccg tcagcagcag tttcagatcg tccgtgatcg cttcccgggc caccgctcga
      901 atcctcccgc tcgatgagcc tacaacgttc cgcaagggcg gcaggcttcc ggaaagaggg
      961 gggaggcaag atcgatgccg tgaaacccgg cgtcgcggcc ccgcgtaggg gagggggaaa
     1021 ggaggagagc tgtcatgcga atcaattcct gcgccgcggt cctggggatc ctgggcctgg
     1081 tcttcttcgg ggggaacgcg gaggctgcca cacggacgga gaacttcgcc aagagctacc
     1141 cgctgggcgc cggcggcgag gtccagctgc gcaatgtcaa tgggggagtg accctggagg
     1201 cctgggaccg caacgaggtc cgggtggagg ccgagaagaa ggcgcggggc cgcgatgacg
     1261 cggcgatccg caaggcgttg caggagatcc gcatcgaggt gtcgcggacc gccggtggcc
     1321 tgcggatcga gacccggctg ccgtcccgca agaacggttt cctcgactgg ctcaccggcg
     1381 acgacgtcag caccagcgtc gagtaccgga tccgggtgcc gcgcaactcg ccgctgggca
     1441 tccagagcgt caacggcggg ctcaagctca ccggcacccg cggccgtgcc cacctgacga
     1501 ccaccaacgg cggcatcgac gtcgcgcagg tgacgggcaa tctgcggctg gagaccatca
     1561 acggcggcat caacgtcgcc tcctcggccg gcgcggtcaa cgcctccagc accaacggcg
     1621 gcatcgaggt gcggctcgcg gacgttcccg atggcagcga cctgtcgttc gagacgatca
     1681 acggcggcat caccctccag ttgccggggg acgtccgcgc cacggtcgac gcggccacca
     1741 ccaacggcca ggtctccagc gacttccagg tgagcggcga caccaagagc cggcgccgcg
     1801 tcgccggcga gatcaacggc ggcggcggca cgctgcgcct ccgcaccagt aacggcagca
     1861 tccacatcgt cgagggctga ggcgtctccg ggcgccggct cctccggtac ggcccttgca
     1921 ccacctgttc ccgggagtga ggcggcgcgg cgcgatccgc gccgtcttct ttcggcaagt
     1981 ctttgaacag gtcccggcag gcgctcgcag gaatgaacaa cacgacctct ccagcgtttc
     2041 ccttgatcca tcgccgagcg caggccggag agccattccg gaaggaggac ccatgacgat
     2101 gaacagacag caatggaccc tggtgagcgg cctcgggatg ggggccggcc tcatgtatct
     2161 gctcgatccc caggccggcg ggcgccgccg cgccctggtg cgcgacaagg cggtctccgc
     2221 ctatagctcg gggagcgacg tgctgcgcaa gacctcgcgc gacctcggca accgctccaa
     2281 gggactggcg gcgggggtgc gctcgaagct gaagaagacg gaggaggacg cggacgatct
     2341 ggtgctccgc gaccgggtgc gctcgaagat gggccgctgg gtctcgaagg ccggagcgat
     2401 cgacgtgacg gtgcaggatt gcgtggtgac gctgcgcggc catgtgcccg aggacgaggt
     2461 ggaccgcctg ctcaagaagg tgcgcaaggt caagggtgtc gaggacgtcg tctgcgagct
     2521 cgcgatcggc gactccgagg acgcctcctc tctgctgagc gagggggagg tgcaggggag
     2581 cacccgcaac tggtcgaagg ccgcgcgcct gctggccggg acggccggcg gcgccctggc
     2641 ggtggcggcg ctgacgcagc gcagcaagat ggctccggcc ctgggggcca ctctggggac
     2701 ggtgggcctc ggcctgctgg ccggcggcgt cggcggctcc aacgtgaacc ccaagcgcct
     2761 gctcaagcgg cgcggtcata gccaggacca gggccagagc ctgacggacg gtgagtccgg
     2821 gcagtcgttc ggcgatatga gcggcgacgg cctcgagtcc aaggcgctcg ccgacagcgg
     2881 cggcgacggc agcgcggtgc ggcaccacga cagccacgag cacggcaagt ccgagagcaa
     2941 gtccgaccgc ttcgactacg agccggtcca cacccactga ggtcccgcgc ggatcctacg
     3001 aggccccggc cgccgtgccg gggccttcct ctaggtggtg tccttactca gtgtaggtgg
     3061 tggaggcgag gagaggacgc ccccggctct cgccgagggt agggggttag caaatgtcgt
     3121 ttatgctgtc agggagacag atgcggcagg aaccggttgc gcacccggcg gcgcaataag
     3181 atacggtgtc gccgcccgca gctttcacca acagtccctc ccccagcttg cgcaccgtct
     3241 ccttcgccag caccagcttc ttcgtcttct tcttcatcgc cactccttag taggtacgcg
     3301 ccacctggcg cgggagcatt gtacccccgg agaggcgcgt tataccgcac gcgagccggg
     3361 catgaacgga gacttgaacc gggaaatttc tggactccgt gctctaccca ccacgtttcg
     3421 attgcatccg ttggcctggg aatctcgccc tcgatgccta cccgcggggg acgccgtgct
     3481 ctgtgacaca atctgccgcc gaaaggagag aggcgcgtgc gcagagtcgg agtcttcggc
     3541 tgggggatcg tcgctcccaa gtcacccaat atcgaggcgt tcgccggcaa tctcgagcgg
     3601 gcggatagct ggctggagcc gttcaacggc ttcggcccgg acaacttcct ggtcggccgc
     3661 cccgacttct ccttcgaggg ctaccacgac tggatcgcgg cgcgcttccc tcccagccgc
     3721 ttccgtcagc tccaggagaa gatggactac ccgaccctct acacggtggg ctccttcatc
     3781 cagtctctgg agcagaaccc cggcctcgag gaggagctgc ggcggctggg caccgccgcc
     3841 cacgtctacg tcggcaccgg cctgggcaac gtcgccacca tcgccgaagg cgccctcacc
     3901 ctcgaccgcg cgcaacgccg ctgggaccgc ttctgggccg agcgcaaccc ggcgatgcgc
     3961 gagcacctcg ccgccatcgc cgaggcggcg ccgaccgacc ccgccgtgcc gccgcggccc
     4021 gagaccgcgc cccccgagga tcgcgacgac gccgagcggg cctggtggca ctactgggcc
     4081 gcccggtccg ccgagctgcg ccagtacctc gccgagctcg ccgagatcga ggggctctcc
     4141 gtccagggcg atgtcgagca cggcaagatg agcgtgatga aggagaagca gcgccgcacg
     4201 gcgcagctcc agaagcgctg gcagacgccg acgcccccct ggcagtcggt ctccgccaac
     4261 ctggtctgga acatctccaa cacgcccgcc gcccaggtct ccatgctcgg ccacatcacc
     4321 ggcctggcgt tcgcgccggt cgccgcctgc tccaccttcg gcgtctgcct caagctcggc
     4381 atggacgcca tcgcccgcgg cgaggccaag gccgtcgtgg tgggcgccac cgacccgccg
     4441 ccccacccga tggtggtggg ggccttcttc gccgcgcgcg tcatctcggc cgacgccgac
     4501 gtctcgaagc cgctgaccgg cctgcgcggc acccacgtgg ccggcggcgc ggtcgtctgg
     4561 atcgtcggcg actacgagta catgaccgcc aagggcttca agccgctggg gatggagccg
     4621 atggccgtcg gggtgagctc cgacgccgac cacatcatca ccccgtcggc cgaggggccc
     4681 accgcggcga tccaccaggc gttcgagctc tccggcatcc gccccgacga gatcgcctcc
     4741 tgggacctcc acgccaccgc cacccccggc gactacaagg aggtgtcgac gatgcgcggc
     4801 gtggtccccg cctcggtgct ggtcaccgcc cgcaagggga ccttcggcca cggcatggcc
     4861 gccggcggcg gctgggagct gaccgcccag tacctcggct acgagcgggg gcagctctac
     4921 ccgacgccgc tccacctcga ggaggtcaac cgccagatcg ccggcgttca cgacctctac
     4981 gtcttcgacc ggccctgtga cgcgccgaag gggccggccg gcaagctctc gatggggatc
     5041 ggcggcatca acgcctgcgt catctcgcga ccctggtgat cctggtgaga gcccggttcc
     5101 tgctatagtt ctcacctcga agtcatgaag gtcctcgtca ccggtggcac cggcttcctc
     5161 ggtcgccgca tcgtctcgga gctcgcgccg cgccactcgc tgcgcctgct ggtccgctcc
     5221 accgcctccc gcgaacgttt tccggcaggg gtcgagttcg ccacgggtga cgtcaccgac
     5281 cgggcgagcc tggagcgggc ggccgccggc tgcgacgcgg tgatccacgc cgccgccctg
     5341 gtcaagatcc tggcgccgcc ggccgacttc gaccgcatca acgtcgccgg gctcgacaac
     5401 gtcctccacg ccgcggagca ggcggggacg gtcgagaagg tggtctacgt ctcgagcttc
     5461 atggccctgg gccccaccga ggcggggccg ggcggcatgc tcgacgagaa ggcggatttg
     5521 cgcgaccgtt cctggatcaa cgactacgag cgcaccaagg cgcactccga ccgccaggcc
     5581 cggaaagcca tcgccgacgg cgcgccggtg gtcatcgtct accccggcgt gatctacggg
     5641 ccgggggaga tgaccgaggg gaacatcgtg gtgcggcacg tcctcgactt ggtgcaccgc
     5701 cgcctgccgg ccttgctggg gtcgcccgcg cggcgctgga actacgtcta tgtggatgac
     5761 gtcgcccgcg gcgtcgccct ggcgctggag aagggcgacc cggggagccg ctacgtcctg
     5821 ggcggcgaga acatcgccca gggggacttc tacgcgctgg tcgagcggct gaccggcgtc
     5881 aaggtgccgt ccctgcgcat gccggacggg atcgccaagg ccgccggcgc ggcgcagaag
     5941 acctgggcgc ggctgcgcgg caccacgccc cagctcaccc ccgacctggt ggaggtctac
     6001 cggcacgact gggcgttcag ctcggcgcgc gcggaggccg agctgggcta ccgctggcgc
     6061 cccctcgccg aggggctcgc cactaccctc acctggttga aggagacagg ccagtggaaa
     6121 ccgtgagcgc gccgccggcg ccttcttcag gggtccgccg ggggctctcc ggcggcgagc
     6181 tgctgcgcaa ggtcgtccat atgtcgatgg ggctgttcgc cctgtcgctg cgctggctgg
     6241 ggccgttctg gggcgccgtg ctggcgctga ccgccctggc cttcaacctc ttcctgttcc
     6301 cgcgcctcgg cggccggcgc ctctggcgcc agcacgaggt gagccggggc tcgtccatcg
     6361 ggatcgtcct ctacccgctg gcggtgctga tcctgatcct cgtcttctat cgccggctcg
     6421 aggtggcggc ggcggcctgg gggatcctgg ccttcggcga cggcatggcc tcggtggtag
     6481 ggatgcagtt cggccgccgc aagctcccct ggaacccggg caagagctgg ctggggtcca
     6541 tcgcctacgt cttcttcggc ggcatggcgg ccgccgccct gttgctgtgg acggcgccgg
     6601 ggcagtactc gccggccttc gccctgacct gcggcctagt gaccgccctc ctcgccgccg
     6661 ggctcgagtc actgccccag gggctcgacg acaacatcgg cgtgccgctg ctgtcaggcc
     6721 tgttcctgct cggcctgctg ctcacccagg ggcgctggga tgacttctgg gcgcaggagg
     6781 ggctcgccac gcggctcctc ctggccgcgg cggtcaacgc cgcgctcgcc ggactggcct
     6841 acgcggcgaa gaccgtggac cgctccgggt tggtggtcgg cttcctggtc gggttctcca
     6901 tcttcgcctt cgccggctgg caggggttcc tgatcctcct caccttcttc atcatcggca
     6961 gcgcctgcac caagatcggc taccggcgga aggcggcggc caagctggcg caggagaagg
     7021 gggggcggcg cggggcgcgg cacgccctgg ccaacgccgg tgtgggcatg gcctgcgcgc
     7081 tgttcctggc gctgacgccg cacccggtgc tcttcggcct ggccctggcc ggcgccttcg
     7141 ccaccgccgc ctccgacacc gcctccagcg agatcggcca gctcctcggc cggcgcacct
     7201 tcctcctcac caccttccgg ccggtgccgc gggggacgga gggggcggtc tcggtcgagg
     7261 ggaccctcgc cggagtgatc gcctcgctgg tcatcggcgg cctcggcgcg ctgctggggc
     7321 tctatccctg gctgggggtc gccttcgtgg cggtggccgc gttcgtcggc accaccttcg
     7381 agagcctggt gggggcggcg ctcgagaagc gcaacctcct ggacaacgaa gccctgaatt
     7441 tcctcaacac cctggtcggc gccctggtgg ccgccgcctt cgctccgctg gtggttccat
     7501 gatcagccag accgaacgtg tcggggcgcc cgccccgtcg aagctcagcc tctacaccca
     7561 gctcgcgcgg ccgttcaccc tgctgccgcc gctcctgggg atcgtctcgg gcgccgtctg
     7621 cgccttcggc tcggtgcaca accccgatcc cctgaaccgg ctgaccctgt cggtgatcct
     7681 caccgtgttg ctgggctccc tctgcgccag cttcatgaac gcggcgtcca acgccatcaa
     7741 ccagatctac gacctggaca tcgaccggct caacaagccg aaccggccgc tggtgaccgg
     7801 ggcgctgtcg gtgcgcgagg tctgggggtt cagctggatc ttctatgccc tggcgctgat
     7861 cccgacctgg ctggtggtgc cgtacccgta cgagaccttc atggagaagc tcacggcgcc
     7921 ggtccggtac cacgagacct tcttcatcta tctggtgggc ctgctcttca ccttcatcta
     7981 ctcggcgccg gagctggggc ggaccaaggc caaggggatg ctggccaact tcaccatcgc
     8041 cattccgcgg gggtgcctgc tcaaggtcgc gggctgggcc atggtggcga gcgtctgggc
     8101 cacggagccc tggtacatcg gcgcggtctt cgccttcttc ctgatcggcg ccgcctcgac
     8161 caaggacttc gccgatatcg aaggggacgc ggcgggcggc tgcaagacgc tgccgatcct
     8221 ctatggagtg gagaaggcgg cgtggatcat cagccccttc ttcgtcctcc cctggctgct
     8281 gctgccgctg ggcgccttcc tgccggaccc gcagagcccg gagcatccga tcctcaccgg
     8341 caacccctgg atgctgaccg gcctgggcgt gctgctcacc gtctggggca tctacaccgt
     8401 ctacctcctg ctgcgcgacc ccgagtcgct caccaagacg gagaaccacc cctcctggaa
     8461 gcacatgtac ctgatgatga tggccgccca ggtggggttc gcgctggcct acctgttctg
     8521 aggctcgtcc ggcaggctgc gtggcacgta gattgaacgt agactggtag agaggcagcc
     8581 gcctccgctt ttctctttcg atcgagggat ctcgtatgcc tgacgactac atgacgacgg
     8641 acgagccgct gaagcgtccg cttgccgacg agctctcggc cgccgtggcc gaggatctgg
     8701 agacgttcga ctccgcccag gccgagcgcg actccgacga cgccttcgcc agcgggagcg
     8761 ccggcgaccg cgagctgccg agcaccgggc tcctctcctt ctacgaccgc ctgcgcgacc
     8821 ggatcctgag gacggccgag aagcgcggcg gcaagctgag cgaggggacg ctcaaggcgc
     8881 tcctcctggt gccggacgtc ttcatcctgc tggtccgcct gaccctggac aagaacgtgc
     8941 cggcctcgac ccgcgccatg atcggcggcg ccctggccta cttcgtcctg ccggccgacc
     9001 tgctgcccga gatgctcctg ggcggcgtcg gcttcatgga cgacctggtc ctggcgacgg
     9061 ccgtcctgtc gcaggccttc agcggcgacc tggagcccta cgcccgcaag cactggagcg
     9121 gcccggacga cctgcgggtc gttctgcagg acgtgaccac gaccgcccag tccctcctcg
     9181 gccagaacct ctacggcaag ctccgcggcc tgatgtcccg ccgcggcatc caggtgcagg
     9241 aggacgaccg ctacgaagag gaagaggatg gctacgactc gggctacggc gaagccccgc
     9301 cgcgcccccg tcgctgagcg gcgctgagcg cctccacctt ccaggcttcg gccccggggc
     9361 tcccccgggg ccgaagcgct tcagggtccc cattgaccgg cacgggagca cctttcttca
     9421 tggggtggtg catgccccca tctctggaca aggaggatcg tatgaagaag gctctgctct
     9481 ggtccgtgtt cgcctggatg ctctgcaccg gtttggccgt cgccggagag ccggtggcgc
     9541 ctctgccgca gactccacag ccggcgatcg cgggcgagtc ctgccatgga cagccggtat
     9601 cgccgctggt ggcggacgca gcctcgacgg tgcccgcgcc cctcgacctc ttcacccccg
     9661 agccgcagca ggcggccatc tgccccctga tcggctgcgt cgatgaccag tactgccgcc
     9721 gcgaccggga ctgcacggcc gcgccgggcg gcgtctgcaa cctcttctgc ccgacccggg
     9781 gctgctgctc gtacaccggg tagccgctcc tgcccgcccg tcccccggac ctgcggtctc
     9841 gggggacggc accctcccga attactcgcg gagcgggtcc aggcgctcgg gccgcgtgcc
     9901 gtgccggcgg atcagctcgt ccaggatctc gcgcaccggc tcgaagccgc cgtcggggaa
     9961 gttgtcgggg ttgtcccaga actcggccag cgcgccggtg ccccagcgct cgtagaccgc
    10021 caccttgagc at
//
LOCUS       DASPJI010000116         6155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_808593, whole genome shotgun sequence.
ACCESSION   DASPJI010000116 DASPJI010000000
VERSION     DASPJI010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808593"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3389)
                     /locus_tag="VEL74_03190"
     CDS             complement(<1..3389)
                     /locus_tag="VEL74_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG61561.1"
                     /translation="MTWPPSHSAPTLVEILRQRAASQAAKIAYRFLADGEEEVASLTF
                     AELDRRARAVGAYLQESGAAGERALLLCPPGLEFVVSFLGCLYGGVIAVPAYPPRANR
                     PDARLAAIVKDARPRFALTNPALAELAPSFAAQVPGLREVRWVATEELDAEWAERWTP
                     EAEPAAMAHRTAFLQYTSGSTATPKGVMVTHGNLVHNERMIQLAFGQSEDSVIAGWLP
                     LYHDMGLIGNVLQPLFAGASCVLMAPVAFLQQPVRWLRAISRYRATTSGGPNFAYELC
                     ARKIEPAALAGLDLSSWQVAFNGAEPVRAETLRLFSETLAPYGFRSEAFYPCYGLAEA
                     TLFVTGGTPGQQAAVREVDAEALGRNEAVPAALGRPSRMLVACGGAWLGQDVHIVDPE
                     SGETLPEGRVGEIQVGGESVAAGYWERPDATRETFGSGFLRTGDLGFLAGGELYVTGR
                     VKDLVIIRGRNHYPQDIELTAERSHPALRPGGGAAFSVEVDGEERLVIVHEVERIWRR
                     DLHGVIEAVRRAVAEEHEVPVHAVALIKPNTLPKTSSGKVRRRSCREDFLAGRLDPLD
                     AWQAGEARPGEAPAAGVAPRTPTEERLALIWCEVFEVAAAGVEDGFFEMGGDSLRATQ
                     MVSRINEAFGVELPLDSLFSSPTIAGLAALLDGMAAPAVPEAPPLRAVPREGSAPLSF
                     AQRRLWFLHQLDPENPVHNIAAFLRLSGVLDVAALARSLDGIVERHEALRTTFRGDCT
                     EPSQVVAPPSSLLLPVIDLMALPESRRRMEEEGIAAAVARLPFALDQGPFVRAVLTAG
                     GPRDHGLVLSWHHIAADGWSLGVFVRELTALYQGAPLPDLAVQYADFAYWQRQWLRGE
                     ALEARLAFWRQVLHGPPPVLELPTDRPRPSVLSHRGAHHEQLLPERLTWPLTALAREA
                     GATPFMALLSGFAALLHRSTGQQDLVLGTPISGRNRRELEDLIGVFINNLVLRIDLGG
                     AGGDPSFRQLLQRVRETALAAYAHHDLPFETLVDALHTERDLSRTPLFQILFVEQSAP
                     LRRLELPELTLEPSEIDLGTARFDLALAMAPVDAGFLGTWKYSTDLFDAPTPARMAGQ
                     LENLLAAAVADPDLPLSRLAWLSDTERHQVVLGWNDTRMEYR"
     gene            complement(3441..4214)
                     /locus_tag="VEL74_03195"
     CDS             complement(3441..4214)
                     /locus_tag="VEL74_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG61562.1"
                     /translation="MRLTEVKAVVTGAAGGLGGYFALELVRAGARVAAGDTNAAGLRR
                     LVSEADGLAGQLVAGKLDVTDEASVESFYSSSVAEIGGLNVLINSAGIIRDGTLLTRE
                     GDQFTRLPVAQWRKVLDVNLTGPFLMTREASLHMAGSEVQEDRVIINLSSIARAGNPG
                     QANYAASKAGLDAATRTWALELAGLGIRVGGIAPGVTSTTFLEGIADEALARLTGEIP
                     LGRLGQPEDIWRAVRFIIECEFFTGRVLEVDGGSTMGKA"
     gene            4367..>6155
                     /locus_tag="VEL74_03200"
     CDS             4367..>6155
                     /locus_tag="VEL74_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HYG61563.1"
                     /translation="MSQLNQDLRFAFRNLRKQPSLALAAILTLALGIGVNTGIFSIVD
                     SVLLTPPPFREPDRLAIVWGSNPELAKMLAVEDKLPASNALLYDWQKASRSFESLALL
                     QANRMVMTRQGEPQQLGAVSVTGDFFKVLGTSPLLGRTIEPADDNLGSPTVVVLSHNL
                     WQRSFSGDRGVIGKVVTLSNNPFTVIGVLPPRFTFPRGGSEMPAGFGFSPEPDIWVPL
                     ALTPEGRQDRGGRGSVVIGRLKPGVDLATAETEIRTITQRFGETYPQTDAGWSARLQP
                     LTEQMLGNLRPALLVLWAAVGLVLLIACANVASLLLARAASRQKEIALRMAMGAGRRR
                     VVSQLLTESALLSLLGGLLGVALAWYGLRAFEAFVPAGLVGAATFSLSLRALAFTAAL
                     CLLATVLAGLVPAFQMTRPDLAGSLREGTRAGAGTVGSRRTRSGLVVAEVALAVLLLV
                     GAGLLLRSFVRLLNVDPGFRTEQVLAFELGMPPDRVPPAERIPFLDRVVDQVRTVPGV
                     ESVALISDLPMGGNEGFTNFTIEGRPEPTPEEALTQVAALRTVSAGYFEIMEIPLRRG
                     RLLAATDRRETLPVGVIDEALAGTFFPNED"
BASE COUNT          906 a         2181 c         2160 g          908 t
ORIGIN      
        1 cggtactcca ttcgcgtgtc gttccagccg agcaccacct gatggcgctc cgtgtcgctc
       61 agccaggcca gacgcgagag ggggaggtcc gggtccgcga ccgcggcggc gagcaggttc
      121 tccaactgcc cggccatgcg cgccggcgtc ggcgcgtcga agaggtcggt gctgtacttc
      181 caggtgccga ggaagcccgc atcgaccggc gccatggcga gcgccaagtc gaaccgcgcc
      241 gtaccgagat cgatctcgct cggctccagg gtcagctccg gcagctcgag gcggcgtagc
      301 ggcgcgctct gctcgacgaa caggatctgg aacagcggcg tgcgcgacag gtcgcgctcg
      361 gtgtgcagcg cgtcgaccag cgtctcgaac ggcaggtcgt gatgcgcata ggcggcgagc
      421 gccgtctccc ggacgcgttg caggagctgc cggaagctcg ggtcgccccc cgcccccccg
      481 aggtcaatgc gcagcacgag gttgttgatg aagacgccaa tgaggtcctc gagctcgcgc
      541 cggttgcggc cggagatggg cgtgccgagc accaggtcct gctggccggt ggagcggtgc
      601 agcagggcgg cgaagccgct cagcagggcc atgaacggcg tcgccccggc ctctcgggcg
      661 agcgccgtga gcggccaggt gagccgctcg gggaggagct gctcgtggtg cgcgccgcgg
      721 tgcgacagga ccgatggccg cggccggtcg gtgggcagct cgaggaccgg cggcggcccg
      781 tggagcacct ggcgccagaa ggcgaggcgc gcctccagcg cctcgccgcg cagccactgg
      841 cgctgccagt aggcaaagtc ggcgtactgg acggcgaggt ccgggagggg tgctccctga
      901 tagagggcgg tcagctcgcg cacgaagacg ccaagcgacc agccgtcggc cgcgatgtgg
      961 tgccacgaca ggacgaggcc gtggtccctg ggaccgccgg ccgtcagcac ggcccgcacg
     1021 aacgggccct ggtcgagcgc gaagggtagg cgggccaccg cggcggcgat cccctcctcc
     1081 tccatgcggc gccgcgactc gggcagtgcc atgagatcga tcactggtag cagcagggag
     1141 gacggcggcg ccaccacttg ggacggctcg gtgcagtcgc cgcggaaagt cgttctcaaa
     1201 gcctcgtggc gctcgacgat gccgtccagc gagcgcgcga gcgccgccac gtccaagacg
     1261 ccactgaggc gcaggaaagc ggcgatgttg tgcaccgggt tctcggggtc gagctggtgg
     1321 aggaaccaga ggcgccgctg ggcgaaggag agcggcgcgc tcccctcgcg cggcaccgcc
     1381 cgcagaggtg gcgcctcggg gaccgccggc gccgccatgc cgtcgagcag cgcggcgagg
     1441 ccggcgatgg tcggcgacga gaacaggctg tcgagcggca gctcgacgcc gaaggcctcg
     1501 ttgatgcggg agaccatctg cgtcgccctg agcgagtcgc cccccatctc gaagaatcca
     1561 tcctcgaccc cggcggcggc gacctcgaag acctcgcacc agatcagggc cagccgctcc
     1621 tcggtcggag tgcgcggcgc caccccggcg gcgggtgcct cgccggggcg agcctccccc
     1681 gcctgccagg cgtccagggg atcgagccgg ccggccagga agtcctcgcg gcacgagcgc
     1741 cggcgcacct tgccgctcga cgtcttgggg agggtgttcg gcttgatcag ggcgacggcg
     1801 tgcaccggca cctcgtgctc ctccgccacc gcccggcgca cggcctcgat gacgccatgg
     1861 aggtcgcggc gccagatgcg ctccacctcg tggacgatca ccagccgctc ctcgccgtcc
     1921 acctccaccg agaaggcggc gccgcccccc ggccgcagcg ccggatggct ccgctccgcc
     1981 gtcagctcga tgtcctgtgg gtagtggttg cggccgcgga tgatgaccag gtccttgacg
     2041 cggccggtga cgtagagctc gccgccggcg aggaagccga ggtcgcccgt ccgcaggaac
     2101 ccggagccga acgtctcccg tgtcgcgtcg gggcgctccc agtagccggc cgcgacgctc
     2161 tcgccgccga cctggatctc gccgacccgc ccctccggca gggtctcgcc gctctcggga
     2221 tcgacgatgt gcacgtcctg cccgagccag gcgccgccgc aggccaccag catccgggag
     2281 ggacggccga gcgcggcggg gaccgcctcg ttgcgaccca gcgcctcggc atcgacctcg
     2341 cggacggcgg cctgttgccc cggggtgccg ccggtgacga acagcgtcgc ctccgccagg
     2401 ccatagcagg gatagaaggc ttcgctgcgg aacccgtacg gcgctaacgt ctcgctgaag
     2461 agtcgcagcg tctcggcgcg caccggctcc gcgccgttga aggcgacctg ccaggaggag
     2521 aggtcgagtc ccgccagcgc cgccggctcg atcttgcgcg cgcacagctc ataggcgaag
     2581 ttgggaccac cgctggtcgt cgcccggtag cggctgatgg cgcgcagcca gcgcaccggc
     2641 tgctggagga acgccaccgg cgccatcagc acacaggagg cgccggcgaa cagcggttgt
     2701 aaaacgtttc caatcaaccc catgtcgtgg tagagaggga gccagccggc gatcaccgaa
     2761 tcttcggact ggccgaaggc gagctggatc atccgctcgt tgtgcacgag gttgccgtgg
     2821 gtgaccatca cccccttggg cgttgccgtg gagcccgagg tgtactgcag gaaagcggtc
     2881 ctgtgcgcca tcgccgccgg ctccgcctcg ggcgtccagc gctcggccca ttcggcgtcg
     2941 agctcctcgg tcgccaccca gcgcacctcc cgcagccccg gcacctgcgc cgcgaagctg
     3001 ggagcgagct ccgccagcgc cggattggtc aacgcgaacc gcggccgggc gtccttgacg
     3061 atggcggcga gccgcgcgtc cggacggttg gcgcggggcg ggtaggccgg cacggcgatc
     3121 acgcccccat agagacagcc caggaacgag acgacgaact ccaggcccgg cggacagagc
     3181 aggagggccc gctccccggc ggcgccgctc tcctggagat aggcccccac ggcgcgcgcc
     3241 cggcggtcga gctccgcaaa ggtgagcgag gccacctcct cttcgccgtc ggcgaggaac
     3301 cggtaggcga tcttcgccgc ctgactggcc gccctctggc ggaggatctc gacgagcgtc
     3361 ggcgcggaat gactgggtgg ccacgtcatg atgaaagcct ctcttgaatt tctcgggcgg
     3421 tcccgcaacc gatcagttct tcaagccttg cccatcgtcg acccgccgtc gacttcgagg
     3481 accctgccgg tgaagaattc acattcgatg atgaagcgga cggcacgcca gatgtcctcc
     3541 ggctgtccca ggcgaccgag ggggatctcg ccggtcaggc gcgccagggc ctcgtccgcg
     3601 atgccctcga ggaaggtggt gctcgtcacg ccaggagcga tgccgccgac gcggatcccg
     3661 agccccgcga gctcgagagc ccatgtacgg gtggcggcgt ctaggccggc cttggacgcg
     3721 gcgtagttcg cctgcccggg gttgccggcg cgtgcgatgg aagagaggtt gatgatcacc
     3781 cggtcttcct gcacctcgct cccggccatg tggagagagg cttcgcgggt catcaggaaa
     3841 ggcccggtga gattgacgtc cagcaccttg cgccattgcg ccaccgggag gcgcgtgaat
     3901 tgatcacctt cgcgggtgag gagcgtgccg tcgcggatga ttccggcgct gttgatcaat
     3961 acattgaggc cgccgatctc ggcgaccgac gacgagtaga acgattcgac ggacgcctcg
     4021 tccgtgacgt ccagcttgcc ggcgacgagc tgcccggcga ggccgtccgc ctcggacacc
     4081 aggcgccgca ggccggcggc attcgtatcg cccgcggcca ccctggcgcc ggcacggacc
     4141 agctcgaggg cgaagtagcc gccaagcccg ccggcggctc ccgtcaccac cgccttcacc
     4201 tccgtcagcc tcattccacc tctcgctttc ttccggcact tttgaaacag tcaattcctg
     4261 tcacatacga ccctggcaac cggctcgatc cctggtctgt ctaggctgaa aaatgatagc
     4321 atcccggctt gtttttcccg caggccccga gaacatagag ggaatcatga gccagctcaa
     4381 ccaggacctt cgtttcgcct ttcgcaacct tcgcaagcag cccagccttg cgctcgccgc
     4441 gatcctgacc ctggccttag gcattggcgt caataccggt atcttcagca tcgtcgattc
     4501 ggtgctcctc acgccaccac cgttccgaga gcccgaccgg ctcgccatcg tgtggggctc
     4561 gaacccggag ctggcaaaaa tgctggccgt ggaggacaag ctgccggcgt ccaatgctct
     4621 tctctacgat tggcagaagg cgagtcgctc cttcgagagc ctggcgctgc tgcaggccaa
     4681 tcggatggtc atgacccgac agggggagcc ccagcagttg ggagccgtca gcgtcaccgg
     4741 cgacttcttc aaagtccttg ggacctcgcc cctcctcggc cggaccatcg agccggcaga
     4801 cgacaatctg ggcagtccga ccgtggtggt gctctctcac aatctgtggc agcgctcgtt
     4861 cagcggcgac cgcggggtga tcggcaaagt ggtcaccctg agcaacaacc cgttcaccgt
     4921 gatcggcgtg ctgccgcccc gcttcacctt cccgcgcggc ggttccgaga tgccggccgg
     4981 cttcggcttc tccccggagc cggacatctg ggtgccgctg gccctcacgc ccgaggggcg
     5041 gcaggaccgg gggggccgcg gcagcgtggt gatcgggcgc ctcaagccgg gggtcgatct
     5101 ggccaccgcg gagacggaga tcaggaccat cactcagcgc ttcggcgaaa cctaccccca
     5161 gaccgacgcg ggctggagcg cgcggctcca gccgctgacg gagcagatgc tgggcaacct
     5221 ccggccggcg ctgctggtgc tctgggctgc ggtcgggctc gtcctgctga tcgcctgcgc
     5281 caacgtcgcc agcctgctcc tcgcccgggc ggcctcgcgc cagaaggaga tcgccctgcg
     5341 catggcgatg ggagccggcc gccgacgcgt ggtctcccaa ctgctcaccg agagcgccct
     5401 gctgtcgctg ctgggcggct tgctgggggt cgcgctggcg tggtacggcc tgcgcgcctt
     5461 cgaagccttc gtccccgcgg gcctggtggg ggccgcgacc ttctctctca gcctgcgggc
     5521 gctggccttc accgcggccc tctgcctcct ggccacggtg ctggcgggcc tggtcccggc
     5581 gttccagatg acccggccgg acctcgccgg ctcgttgcgc gaaggcaccc gggcgggagc
     5641 cggcacggtg ggcagccgcc gcacgcggag tggcctggtg gtggcggagg tggctctcgc
     5701 ggtgctgctg ctggtcggtg ccggcctgct gctgcgcagc ttcgtccgcc tgctgaacgt
     5761 cgaccccggc ttccgcaccg agcaggttct cgccttcgag ctgggcatgc cgcctgaccg
     5821 cgtgccgccg gccgagcgca tccccttcct cgatcgggtc gtcgatcaag tgcggacggt
     5881 gccgggtgtg gagtccgtcg ctctgatctc cgacctgccc atgggaggca acgagggctt
     5941 caccaacttc accatcgaag ggcggccgga gccgacgccg gaggaggcgc tcacccaggt
     6001 ggcggcgctg cgcaccgtct cggccggcta tttcgagatc atggagatcc ccctgcgccg
     6061 cggccgtctc ctcgccgcca ccgaccgcag ggagacgctg ccggtgggcg tcatcgacga
     6121 ggccctggcc ggcaccttct tccccaacga ggatc
//
LOCUS       DASPJI010000117         8342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_67655, whole genome shotgun sequence.
ACCESSION   DASPJI010000117 DASPJI010000000
VERSION     DASPJI010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67655"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VEL74_03205"
     CDS             <1..434
                     /locus_tag="VEL74_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019870618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61564.1"
                     /translation="IMMGPETAAHHDLIIRFGGPLAWSQHLNSAGMGAGLASVRIHES
                     PELPRLQGKLRENLALFDSLIGTEQSGDDFSIRMVVIGSEAAAADASGKIFERGFYTS
                     PVFFPIVERGKAGLRVMLRADNRPEDIRTFCELVRDAVELD"
     gene            434..1357
                     /locus_tag="VEL74_03210"
     CDS             434..1357
                     /locus_tag="VEL74_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HYG61565.1"
                     /translation="MNGGAVPERLVCRSMLMTCALYPRQYGKAEETGADVGTIDLEDS
                     VPAARRDEARRLALSFFKGRREPCLRTLRTLRINSLRTPDGLRDILALLESGARPEAL
                     LVPKVDSAQDLLILQELLGRALTDTLFLVVIETAAGLCAVEEIAAATPRVRALVFGAA
                     DFSSEMGTSMSWEHMLHARSRILVAAARAGIPAMDAPCFEVQDEMALRVEVRKSQELG
                     FHGKAAIHPCQVAAINEGFTPGPEAIDRARRIVELTERRAGQICVLDGDMIGPPMVLA
                     ARRLLSQVETLEAAGKLEPVNGLELAGRSPL"
     gene            1381..1905
                     /locus_tag="VEL74_03215"
     CDS             1381..1905
                     /locus_tag="VEL74_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HYG61566.1"
                     /translation="MDQIRAYKKVGERRWREARGLYFEDVEVGAIVEHRPGRTVTEAD
                     NIWQSLLAMNLHPLHIDSAYCAQTEWKRPLMSSLVTFSLVAGMSVQSTSAKGLANLGW
                     DKIRLLAPVFAGDTVYAESEYLRKRLSKSRPHQGLVTCATRGVKADGTLFLTCRRTFL
                     LPTREHGLEDGAGY"
     gene            1892..2911
                     /locus_tag="VEL74_03220"
     CDS             1892..2911
                     /locus_tag="VEL74_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HYG61567.1"
                     /translation="MPATSARTHVVICDGFGGPEVLKIVEAPRPVPGPDELLVRVRAA
                     ALNNADLLQRDGSYSPPPGESEILGVEIAGEVEEMGERVSGFQRGQRVFGLVGSGGYA
                     EHCLLDAGMAIPVPDGWSWTEAAAVPEAYFTADTTLFELGGLAAGQSVLIHAAGSGIG
                     TVCIQLARFTGARVLCTAGSDEKLARCRELGADAGFNYKTEDWAAEVLRATGDAGDTG
                     GAGVDVVWDVVGATYLARNLAVLRDGGRLVQVGLLTGWETEIDLLPLVLRRLQIKGSS
                     MRPRSLADKRAITRKFRERWLPLLEERRLRPVIDSVFPLERVAEAHARMEAHLNFGKI
                     LLTID"
     gene            2953..3402
                     /gene="nrfH"
                     /locus_tag="VEL74_03225"
     CDS             2953..3402
                     /gene="nrfH"
                     /locus_tag="VEL74_03225"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HYG61568.1"
                     /translation="MRGSTLIAIILGLLLGLAAGIGGYTFLYAKGASYLTNDPAACAN
                     CHVMQSQYDQWLVSSHRSVAVCNDCHTPPGFIPKYFTKALNGFNHSYAFTTGWFHEPI
                     QINERNRQVTEQACRKCHENIVQAIEGPHRPGQELSCIRCHSTVGHF"
     gene            3427..4950
                     /locus_tag="VEL74_03230"
     CDS             3427..4950
                     /locus_tag="VEL74_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HYG61569.1"
                     /translation="MDNDARSREPRHRRGWGLVLGVALVSALAAAGATALLVNIFEHK
                     QEARNPFYRVVEIDDQTEDPAVWGKNFPMQYDQYKKTVDQVRTRYGGSEALPRTPDEA
                     DPRSIVAQSRLEEDPRLKTMWAGYAFAADFREERGHAYMLEDQTFTQRQVVKQQPGTC
                     MHCHASVYLPYKKLGNGDLMAGFEKMNQMPYQEARKLVTHPVACIDCHDPETMQLRVT
                     RPGFIEGIRTLKAAQGVENFDVNTMATRQEMRAYVCGQCHVEYYFKGPEKRLTYPWHK
                     GLKVDEILAYYDEEGFKDWEHAETGAPALKAQHPEFEMWNQGIHARSGVACADCHMPY
                     TRVGSLKISDHHVRSPVLNINNACQTCHKWPEEELRARIHTIQDRTFAMRNLAMDALV
                     ALINDLKIARQAAAPVEQIARAQDFQRKAQFYLDFIEAENSMGFHADQEAVRILAQSI
                     DFARQGQMALRGVPGLPTVQTVSAPPPAQRTTPEVGGVAPGREGGAAPGAAPGRTGG"
     gene            complement(4987..5928)
                     /locus_tag="VEL74_03235"
     CDS             complement(4987..5928)
                     /locus_tag="VEL74_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG61570.1"
                     /translation="MSRPGPPHPPDEPPPLDRLLFDSPLLRIGAFRAPPSHPRFHDSG
                     PAENDIFVFPRTSVWLQHEGGKRFVVDPNSVTFYNKGQVYRRGKVSEEGDHCEWFAFR
                     PQVLVDAVRAHDPAVVEHPDQPFSFSHGPSDPRSYLVQRLLVRQLMKGELIEPLRVEE
                     TMLHVLARLLGNAAEMYEWRSLPATVAIGGDLVEETKAVLARSFREPLSLQEIAGRIG
                     FSVFHLCRLFRRGTGTTLHGYRNHLRLRTALEEVADPRADLSALGLDLGYSSHSHFTS
                     AFRQAFGTTPSTLRRTASGPRVRELASRLLAEPPPRA"
     gene            6128..6436
                     /locus_tag="VEL74_03240"
     CDS             6128..6436
                     /locus_tag="VEL74_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61571.1"
                     /translation="MIATGLLVLALHGAAAQETGAAPEAGTLQAFLQSLQPPAASLVT
                     PEAAGTPEALSLATPPKGVSCCRAYPSCCSTVATAEACTDLGGEPHSTTQLCALNCCW
                     "
     gene            6521..6703
                     /locus_tag="VEL74_03245"
     CDS             6521..6703
                     /locus_tag="VEL74_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61572.1"
                     /translation="MKKRFEKKLSLNRETLRRLTGGWREVDAGLPTTPIGTCPASDPA
                     ACGTGTGGTSTVECTQ"
     gene            6852..>8342
                     /locus_tag="VEL74_03250"
     CDS             6852..>8342
                     /locus_tag="VEL74_03250"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006140844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HYG61573.1"
                     /translation="MAANPTTAILESPAASTQAGPAPTSSPRIGPEQLASLAARVSLA
                     SPAGETFTVHSPLDGRRIGEIPKATPEDVRAAVKRARVVQDDWAHRTFAERAEVFLRF
                     HDLVIERQEEVLDLIQLESGKARKHAFEEVADSAIVARYYAINGEDHLAPKRRKGALP
                     GLTLAWEHHHPVGVVAIISPWNYPLSLAVTDAVAALLAGNAVVLKPDHQTSFTALWAA
                     ELLEEAGLPAGLFQVVTGEGTVLGTPLIESCDFLTFTGSTATGRKVARQAGERLIGCT
                     LELGGKNAMLVLADADVEAAVSGAVRGSFANAGQLCISFERIYVHTSIYPLFLERLLQ
                     ETRALRLGAGLDYEADMGPLISRTQLEKVTEHVADAVAKGATVHAGGRARPDLGPYFY
                     EPTILTGVTKEMKLFAEETFGPVVAVYRFESVHDAVERANDSPYGLNASVWTRDTELG
                     HRVATRLRVGTVNINEAYAAAWASVDAPMGGFKDSGLGRRHGAEGIL"
BASE COUNT         1304 a         2965 c         2876 g         1197 t
ORIGIN      
        1 tcatcatgat ggggcccgag accgccgcgc accatgacct gatcatccgc ttcggcggcc
       61 ccctggcctg gtcgcagcac ctcaacagcg ccggcatggg cgcggggctc gcctccgtcc
      121 gcatccacga atcccccgag ctgccgcgtc tccagggcaa gctgcgggag aacctcgccc
      181 tgttcgacag cctgatcggc accgaacaga gcggcgacga tttctccatc cgcatggtcg
      241 tgatcggttc cgaggccgcc gcggcggacg cctcgggcaa gatcttcgag cgcggcttct
      301 acacctcccc ggtcttcttc cccatcgtcg agaggggtaa ggccgggctg cgggtgatgc
      361 tgcgggccga taaccgtccg gaggacatcc ggaccttctg cgagctcgtg cgcgacgccg
      421 tcgagctcga ctgatgaacg gaggagccgt gcccgagcgg ctggtctgcc gcagcatgct
      481 gatgacctgt gctctctacc cgcgccagta cggcaaggcg gaggagacgg gcgccgacgt
      541 cggcaccatc gacctcgagg actcggtgcc cgcggcccgc cgcgacgagg cccgccggct
      601 cgctctgtcc ttcttcaagg ggaggcggga gccctgcctg cggacattgc gcaccctgcg
      661 catcaacagc ctgcgcaccc ccgacggcct gcgcgacatc ctggcgctgc tggagagcgg
      721 cgccaggccc gaggccctcc tggtccccaa ggtcgactcg gcccaggacc tcctcatcct
      781 gcaggagctg ctgggccgcg ccctgaccga caccctcttc ctggtggtca tcgagaccgc
      841 agccgggctc tgcgccgtgg aagagatcgc cgccgccacg ccgcgtgtcc gggcgctggt
      901 cttcggcgcc gccgacttct cgtccgagat ggggacctcc atgtcctggg agcacatgct
      961 gcatgcccgc tcccggatcc tggtggccgc cgcccgcgcg ggcatcccgg cgatggacgc
     1021 gccctgcttc gaagtccagg acgagatggc gctccgcgtc gaggtccgga agtcccagga
     1081 gctcggcttc cacggcaagg ccgccatcca cccctgccag gtggcggcga tcaacgaggg
     1141 cttcacgccg gggcccgagg cgatcgaccg cgcccggcgt atcgtcgagc tgactgagcg
     1201 gcgcgccgga cagatctgcg tcctcgatgg tgacatgatc gggccgccga tggtcctcgc
     1261 cgcccgccgc ctgctgtccc aggtggagac gttggaggcc gcgggcaagc tcgagcctgt
     1321 gaacggtctg gagctggcgg gccggtcccc gctctgaggc acctgccccc gaggagatcc
     1381 atggatcaga tccgcgccta caagaaagtc ggggagcgcc gctggcgcga ggcccgaggc
     1441 ctgtatttcg aagatgtcga ggtcggtgcg atcgtcgagc accggccggg ccgcaccgtc
     1501 accgaggccg acaacatctg gcaatcgctg ctcgccatga acctccaccc tctgcacatc
     1561 gacagcgcct actgcgcgca gaccgagtgg aagcggccgc tgatgtcgag cctggtgacg
     1621 ttctccctcg tcgccggcat gagcgtccag agcaccagcg ccaaggggct cgccaacctc
     1681 ggctgggaca agatccgcct cctcgccccg gtgttcgcgg gcgacaccgt ctacgccgag
     1741 agcgagtacc tgcgcaagcg gctctcgaag tcgcgccccc accagggcct ggtcacctgc
     1801 gccacccggg gcgtcaaagc cgacggcacg ctgttcctga cttgccggcg gacctttctg
     1861 ctccccaccc gcgagcacgg cctcgaagac ggtgccggct actagcgcgc ggacccacgt
     1921 cgtgatctgc gacggcttcg gcggccccga ggtcctgaag atcgtggagg ccccgcggcc
     1981 cgtgccggga ccggacgagc tcctggtccg ggtccgggcg gccgccctca acaacgccga
     2041 cctgctgcag cgcgacggct cgtactcgcc gccgccaggg gagtccgaga tcctcggcgt
     2101 cgagatcgcc ggcgaggtgg aggagatggg ggagcgggtc tccggcttcc agcgcggaca
     2161 gcgggtcttc ggcctggtgg gcagcggcgg ctacgccgag cactgcctcc tggacgccgg
     2221 catggcgatc ccggttcccg acggctggtc ctggaccgag gccgcggcgg tccccgaggc
     2281 ctacttcacc gccgacacca ccctgttcga gctcggcggg ctggccgccg gccagtcggt
     2341 gctgatccac gccgccggca gcggcatcgg cacggtctgc atccagctcg cccgcttcac
     2401 cggcgcccgg gtcctctgca ccgccggctc ggacgagaag ctcgcccgct gccgcgagct
     2461 cggcgcggac gccggcttca actacaagac ggaggactgg gccgcggagg tcctgcgggc
     2521 caccggcgat gccggcgata ccggcggcgc tggcgtcgac gtggtctggg acgtcgtcgg
     2581 tgccacctat ctggcccgca acctcgcggt cctcagggac ggcggccggc tcgtccaggt
     2641 agggctcctg accggctggg agacggagat cgacctcctg cccttggtgc tgcggcggct
     2701 ccagatcaag ggctcgtcca tgcgcccccg ttccctcgcc gacaagcggg cgatcacccg
     2761 caagttccgg gagcgctggc tgcccctcct ggaggagagg aggctgcggc cggtgatcga
     2821 ttcggtcttt cccctggagc gggtggccga ggcccacgcc cggatggagg cccacctcaa
     2881 tttcggtaag atcctgctga cgatcgactg aaaccggcaa accgcttgca ggaggtggac
     2941 gcggcgaacg gtatgcgcgg ctcgaccctc atcgccatca tcctgggact gctgctcggc
     3001 ctggcggccg gcatcggcgg ctacaccttc ctctacgcga agggggcctc ctacctcacc
     3061 aacgacccgg cggcctgcgc caactgccac gtgatgcagt cgcagtacga ccagtggctg
     3121 gtgtcgagtc accgctcggt ggcggtgtgt aacgattgcc acacccctcc agggtttatc
     3181 cccaagtact tcaccaaggc tctgaacggc ttcaaccact cctacgcctt caccaccggc
     3241 tggttccacg agccgatcca gatcaacgag cgcaaccggc aggtcaccga gcaggcctgc
     3301 cgcaagtgcc acgagaacat cgtgcaggcc atcgaagggc cccaccggcc cgggcaggag
     3361 ctgtcctgca tccgctgcca ctcgaccgtg gggcacttct gatccgttcc gaggagagct
     3421 gacgagatgg acaacgacgc gagaagcaga gagccgcggc accggcgggg gtgggggctg
     3481 gtcctggggg tggccttggt ctcggctctg gcggccgccg gcgcgaccgc gctcctggtc
     3541 aacatcttcg agcacaagca ggaggcccgg aaccccttct accgggtggt ggagatcgac
     3601 gaccagacgg aggatccggc ggtctggggg aagaacttcc ccatgcagta cgaccagtac
     3661 aagaagacgg tggaccaggt gcgcacccgc tacggcggca gcgaggccct gccgcgcacg
     3721 cccgacgagg ccgacccgcg ctccatcgtg gcccagtccc gtctcgaaga ggacccgcgc
     3781 ctgaagacca tgtgggccgg ctacgccttc gccgccgatt tccgcgagga gcgcggccac
     3841 gcctacatgc tggaggacca gaccttcacc cagcgccagg tggtcaagca gcagcccggc
     3901 acctgcatgc actgccacgc ctcggtctat ctcccctaca agaagctggg caacggcgac
     3961 ctcatggccg gcttcgagaa gatgaaccag atgccctacc aggaggcgcg caagctcgtc
     4021 acccatcctg tggcctgcat cgactgccac gaccctgaga ccatgcaact gcgcgtcacc
     4081 cggccgggct tcatcgaggg gatccgcacc ctcaaggccg cccagggggt ggagaatttc
     4141 gacgtcaaca ccatggccac gcggcaggag atgcgcgcct acgtctgcgg ccagtgccac
     4201 gtcgagtact acttcaaggg gccggaaaag cggctgacct acccctggca caaaggcctc
     4261 aaggtggacg agatcctcgc ctactacgac gaggaggggt tcaaggactg ggagcacgcc
     4321 gagacgggcg cgccggcgct caaggcgcag caccctgagt tcgagatgtg gaaccagggc
     4381 atccacgcgc gctcgggcgt cgcctgcgcc gactgccaca tgccctatac gcgagtgggg
     4441 tcgctcaaga tcagcgacca tcacgtgcgc agcccggtgc tcaacatcaa caacgcctgc
     4501 cagacctgcc acaagtggcc cgaggaggag ctgcgggcga ggatccacac gatccaggac
     4561 cgcaccttcg cgatgcgcaa cctggcgatg gacgcgctcg tggctctgat caacgacctg
     4621 aagatcgccc gccaggccgc cgcgccggtc gagcagatcg cccgcgccca ggatttccag
     4681 cgcaaggccc agttctatct cgacttcatc gaggcggaga actccatggg cttccacgcc
     4741 gaccaggagg cggtgcgcat cctcgcgcag tccatcgact tcgcgcgcca ggggcagatg
     4801 gcgctgcgtg gcgtgccggg cctgccgacg gtgcagaccg tatccgcgcc gccgccggcg
     4861 caacggacga cgcccgaggt gggcggggtc gcgcccggcc gggaaggcgg cgccgcgccg
     4921 ggagcggcgc cgggacggac gggcgggtag gcccgggtac gggcggccag ccttacggga
     4981 gacgcttcac gcccggggag gcggttcggc gaggaggcgg ctggcgagct cgcgcacccg
     5041 ggggcccgac gcggtccggc gcagggtgga cggcgtggtg ccgaaggcct gccggaacgc
     5101 cgaggtgaag tggctgtggc tggagtagcc caggtccagg cccagcgcgg agaggtcggc
     5161 gcggggatcg gccacctcct ccagggcggt gcgcagccgc agatggttgc ggtagccgtg
     5221 cagcgtcgtc ccggtgccgc ggcggaacag ccggcagagg tggaagacgg agaagccgat
     5281 gcggccggcg atctcctgga gcgacagcgg ctcccggaag ctccgcgcca gcacggcctt
     5341 cgtctcctcc accaggtcgc cgccgatcgc cacggtggcc gggaggctcc gccattcata
     5401 catctcggcg gcgttgccga ggagccgggc gaggacgtgg agcatcgtct cctcgacgcg
     5461 caggggctcg atgagctctc ccttcatgag ctgccgcacc aggagccgct ggaccaggta
     5521 gctccgggga tcgctcggcc cgtggctgaa cgagaagggc tggtccggat gctcgacgac
     5581 cgcgggatcg tgggcgcgca cggcgtcgac caggacctgc ggccggaacg cgaaccattc
     5641 gcagtggtcc ccctcctcgc tcaccttgcc ccggcgatag acctggccct tgttgtagaa
     5701 cgtcaccgag ttgggatcga cgacgaatcg tttgccccct tcgtgctgca gccagacgct
     5761 ggtgcgcggg aagacgaaga tgtcgttctc ggccggcccg gagtcgtgga agcgcggatg
     5821 cgacggcggc gcgcggaagg cgccgatgcg gagcagcggg ctgtcgaaga gaagacggtc
     5881 gagcggcggc ggctcgtccg gcgggtgggg cggtcccggt ctgctcatgg tcgtatccct
     5941 gtcggctctc tccattgtat acgtacgccg gcctcccgaa gttctgatca cttttccaaa
     6001 gcaagatctc gatagcgcgg gtgttcgttc ccgcgcagac tggcgatgga tcacggctcc
     6061 gccggggcat cggtgccagg cggagggccg gatcaggagg aataaagtga tgatttggaa
     6121 aaagtgtgtg atcgctaccg gtctgctcgt cctcgccctg cacggcgccg ccgcgcagga
     6181 gacgggcgcc gcgcccgagg ccggcaccct ccaggcgttt ctccagagcc tccagccgcc
     6241 ggccgcctct ctggtcaccc cggaggccgc cgggaccccg gaggcccttt ctctcgccac
     6301 tccccccaag ggggtgtcct gctgccgcgc ctacccgagc tgctgctcca cggtggctac
     6361 cgcggaggcc tgcaccgacc tgggagggga gccccactcc accacccagt tgtgcgccct
     6421 gaactgctgc tggtgacacc cgctccaagc gggcggaggg cttcggccct ccgcccgcac
     6481 cggcgttaag atggggctcc cattccaaag gagagcaccc atgaagaaga gattcgagaa
     6541 gaagctgagc ctgaaccgcg agaccctgcg gcgcctgacc ggcggctgga gggaggtcga
     6601 cgccggcctg cccaccaccc cgatcggcac ctgccccgcg tccgaccccg ccgcctgcgg
     6661 caccggcacc ggcgggacca gcacggtcga gtgcacgcag tgagcagcag cccggccgga
     6721 ggccccctcc ggccggggct ttgccgcacc gcgttaatcc tttttgccgc agtttgacgt
     6781 aaatcccccg ctgtggcact ctacggagtg ggggagcggg gggctcctcc gactcacgga
     6841 ggtctagcgg catggcggcg aatcccacga cagcgatcct ggagagcccg gcggcatcga
     6901 cacaggccgg gccggcgccc acctcgagcc cgcggatcgg cccggagcag ctggcgagcc
     6961 tggcggcgcg agtcagcctg gcgagcccgg ccggggagac gttcaccgtc cattcacctc
     7021 tcgacggccg gcggatcggt gagattccga aggcgacgcc ggaggacgtg cgggcggcgg
     7081 tgaagcgggc gcgggtggtc caggacgact gggcgcaccg cacgttcgcc gagcgcgccg
     7141 aggtcttcct gcgcttccac gacctggtca tcgagcggca ggaggaggtt ctcgacctca
     7201 tccagctcga gtcgggcaag gcgcgcaagc acgccttcga ggaggtggcc gactcggcta
     7261 tcgtcgcccg ctactacgcg atcaacggcg aggaccacct ggcgcccaag cggcgcaagg
     7321 gggcgcttcc cggcctcacc ctcgcctggg agcaccacca tccggtgggc gtcgtcgcca
     7381 tcatctcccc ctggaactat ccgctctccc tggccgtgac cgacgccgtg gccgccctcc
     7441 tggccggcaa cgccgtggtg ctgaagccgg atcaccagac ctcgttcacg gcgctctggg
     7501 ccgccgagct gctggaggag gcggggctgc ccgccgggct cttccaggtg gtgaccggcg
     7561 aggggaccgt gctgggaacg ccgctcatcg agtcctgcga cttcctcacc tttaccggca
     7621 gcaccgccac cggccgcaag gtggcgcggc aggcggggga gcggctcatc ggctgcaccc
     7681 tggagctcgg cggcaagaac gccatgctgg tcctcgccga cgccgacgtc gaggcggcgg
     7741 tctccggcgc ggtgcgcggc agcttcgcca acgccggcca gctctgcatc tccttcgagc
     7801 ggatctacgt ccacacgtcg atctacccgc tcttcctcga gcgcctcctc caggagaccc
     7861 gcgccctgcg tctgggcgcc gggctcgact acgaggcgga catgggaccg ctgatctcgc
     7921 ggacgcagct cgagaaggtg acggagcacg tggcggacgc cgtggcgaag ggggcgacgg
     7981 tccacgccgg cggccgggcg cggccggacc tcggacccta cttttacgag cccaccatcc
     8041 tgaccggcgt gacgaaggag atgaagctct tcgccgagga gaccttcggc ccggtggtgg
     8101 ccgtctaccg tttcgagtcg gtccacgacg cggtcgagcg ggcgaacgac agcccgtacg
     8161 gcctgaacgc cagcgtctgg acccgcgaca ccgagttggg ccaccgcgtg gcgacccgcc
     8221 tgcgggtggg caccgtcaac atcaacgagg cctacgccgc ggcctgggcg tccgtcgacg
     8281 cgccgatggg cggcttcaag gactcgggcc tcggccgccg gcatggcgcc gagggcatcc
     8341 tc
//
LOCUS       DASPJI010000118        15017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_178630, whole genome shotgun sequence.
ACCESSION   DASPJI010000118 DASPJI010000000
VERSION     DASPJI010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15017)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15017
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178630"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VEL74_03255"
     CDS             <1..732
                     /locus_tag="VEL74_03255"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HYG61574.1"
                     /translation="VRTFFQTYYAPNNAVLTLTGDFDPEDALRRIETWFGDIPPGPPV
                     PPVVAELRPLGGERREVREDNVRLPRIYLGFRAPAVGERLWYAADLLAGILTGGKSAL
                     LYRDLVYDRQIAQDVSASVSPYEEVATFLVAATARPGVRVETLEAALLEHLDRLAASP
                     PAPADLERARNRTLMDYFSSLQKIETRADQFGMFTTYLDDPGALARESEIFGELTGQD
                     VQDYTAGWCRESERVVVTFVPRGEA"
     gene            729..2093
                     /locus_tag="VEL74_03260"
     CDS             729..2093
                     /locus_tag="VEL74_03260"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYG61575.1"
                     /translation="MIDRSLPPPPGPLRPFRFPAFQRARLANGLTVFAARQGSVPLVS
                     LELVTQAGGQHSPAGQAGLASLTASLLDEGTARRTALEIAGEVENLGGYLVTGADWDV
                     GYLGVGLLSSHLERGLELLAEVATAATFPEAEIERLRRQRLTEILRRTQDPSAVADER
                     LLREIYHGTVYEGSLLGTEESVRGLGRDAITAFFHRHYGMDGSFVIAVGDLDPGEVTR
                     RVEAVLGGAGGTAPPPPGIRPAPLGGVAVHLVDRPGAAQAELRLGHPCISRTHPDYVP
                     LMVANALLGGKFTSRINLNLRERHGYTYGASSRLVGRQGPGPFLVSSAVATESAGAAA
                     REVLSELRRLREEPVEVAELEETLSYLTGVFPYTLQTIGEVAKRLETLGTYGLPDDYY
                     ESYLERIGAVTREDILEAARRHIHPERIAVVAVGPAEALERQFEGLGPVTVWSPQAEP
                     QLAG"
     gene            complement(2090..4378)
                     /locus_tag="VEL74_03265"
     CDS             complement(2090..4378)
                     /locus_tag="VEL74_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYG61576.1"
                     /translation="MLPLTPTKLGRYEILDEIGKGAMGVVYLARDPLIGRLVALKTFR
                     MGFSVRDQEADQFRARFIREAQSAGILSHPNIVTIHDVVEGAGDGLSFIAMEYVRGTN
                     LKMVMQADPSLPLGRVADIISQVAEALDYAHSQRVIHRDVKPANIIMTEDGRVKITDF
                     GIARLDTSNLTQEGQLLGTPNYMAPEQVQGKEVDHRADIFALGVVLYEMLTRHKPFQG
                     ENLTVVSHRIVYDQFTPLREYARNVPPGVEKIISKALEKDPNRRYQRAREMADELKKL
                     VAESLNGDELNETQSLSSTMVIPSGSLPLGASSTASPSTVGPAPGSVGLVGSAGAAGP
                     VAAPPARKPLWGRLFGGGKGAPAPEPIPVAPPPPLVAVPPAAPDLLSGTGTSGTGTGS
                     GSRPAAPAGPAAVPPPPVMTPANDDGSAASATGSSISFTPPPPLPMPAPPRAVPPAAA
                     GSSRKRSSQLIGAVIAALFLGFIIFWGIARTRDSRQAAETPVVAATPQPEAAIDVAKL
                     FEQGNALMRRGRFSEAASVYGLAEPHARNHPDIPRLRQEALRMEAEFNGLKAQEEAVE
                     GHLAKAKAEWRLRRYPEAIAAASLVLEARPWEREASDIARRARIEVDRQRGTQMAAGG
                     ELTPQPAQSAEGSAAETPAGAETSSSAAPQTGRLQMSLRIDAPAAEGSVRVYLGSTKI
                     WDQPFDVGAGLLKRRRSRDFSKGYEVPAGENKLLVYYSPKDLEVTGTQQPVTVDVPGG
                     GVRRIDIRITQSGRLYAVGEQS"
     gene            4563..6659
                     /gene="fusA"
                     /locus_tag="VEL74_03270"
     CDS             4563..6659
                     /gene="fusA"
                     /locus_tag="VEL74_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HYG61577.1"
                     /translation="MQVDSPEKIRNIAVAGHNDTGKTTLVSALLYAGGVTTRLHRVED
                     GNTLTDFDHEETERGISIGLAPCFVPWQQTKINLIDCPGYGIFFTEAKAGMRAADAVL
                     LCVNSVAGVEVNTEKIWSYAEEIDLPVMIHLTKMDRERADFDRALEGLHKSLGRLVIP
                     VQIPIGREQGFTGVVDLVRQKAYKFTRDGNGKAEAADIPADLAGEVEAHRARLMEAVA
                     EGDEALMERFFEEGTLPQEDLERGLRLAVRNRQVFPLTVSAGSHGIGPSALLDSFVAL
                     LPSPADRKPFPAQTVGGEDLEVGTDPAGPLAALVFKTLSDPFTGKISIFRVVSGTLSQ
                     DSNAYNCRAEENERIGHTMLLQGKQVTNVPRLVAGDIGGLAKLKVTSTGDTLCAKERP
                     VRLGWITVPEAAMAFAVEPKSKGDEEKIGDALHRLMDEDVSLHTGRDPETHEYLLSGT
                     GQLHIEIAVSKLKHRYHVEVVLHPPKVPYRETILRPADGHGRHKKQSGGRGQFADCKI
                     KIEPLPRGEDFSFADEIFGGSIPQNYRPAVEKGIQEARRRGYLAGYPVVDFKVRLLDG
                     QYHDVDSSELAFKIAGSLAFKDAMANAKPTILEPIMKVEVVASDEFTGDIMGDLSHRR
                     GRPQGMDSMNGNQVIKATVPMSEMLNYAPALRSMTQGRATFHMEFSHYEEVPRPVQEK
                     LIAEAAKHRQEEQQHH"
     gene            6752..7309
                     /gene="efp"
                     /locus_tag="VEL74_03275"
     CDS             6752..7309
                     /gene="efp"
                     /locus_tag="VEL74_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HYG61578.1"
                     /translation="MIQATQMRVGMCILFEGNICRIMQVQHVTPGNWRGFVQARMRNI
                     KSGNSFEHRFGSTEKVERAILDTSPMEYLYSDPSGHHFMNQETYEQITMEDETLGDTM
                     LYLLPNTVISVDFYEGQPVGIDLPNTVTLEVVETEPGLKGATASSSYKPAKMETGLTV
                     QVPPFIEVGTKILIDTRENKYLSRA"
     gene            7306..8013
                     /locus_tag="VEL74_03280"
     CDS             7306..8013
                     /locus_tag="VEL74_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HYG61579.1"
                     /translation="MSPEQLDQIVLGLTWFVVFLFSTTLHEAGHAFAAYRLGDSTAYE
                     GGQVSLNPLPHIRREPFGMVLVPLITFATMGWMMGWASAPYDPTWAHRYPRRAALMAL
                     AGPLGNLILVLVAGLALRAGLAMGVFVPAPQDFFTYLVSPAGDSAAAGAIVPLSILFS
                     LNLLLFVFNLLPLPPLDGSAIVPAFMSNTMVARYNAFLHQPMLSLLGLLVAWKVFPFI
                     FQPVLILALRLLYSGLG"
     gene            complement(8017..9780)
                     /locus_tag="VEL74_03285"
     CDS             complement(8017..9780)
                     /locus_tag="VEL74_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020596282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYG61580.1"
                     /translation="MKTRRDPWVGRTAVALTAVIAACCLAVGAQEKQEQNTRKAEAPA
                     AAPQTPPPSPPPPAAWAPQEVPKAPPRAEGEGPYGRLILRGVTLIDGTGAPPLGPVDI
                     VIEKNRIADIVPVGYPGVPIDPQSRPLLGEGGRELDLQGMYVLPGFVDMHGHTGGRAQ
                     GTTAEYVYKLWMGHGITTVREPGSTNGIDWTLDEKAKSARNEITAPRIESYVVFGMDR
                     DTPFKTPDEVRQWVAGLATRGADGIKLFGERPEMLKAAIEEAKKRGLRTAAHHAQLYV
                     AQADVLDTARWGLTTMEHWYGLPEALFGDRSIQDYPSDYNFLDESHRFGEAGRLWEQA
                     APPFSERWNKVMDELIALDFTLDPTLTIYEASRDLMRARRAEWHEEYTLPSLWDFYTP
                     SRKAHGSYWYNWTTGDEVAWRNNYRLWMTFLNEYKNRGGRVTTGSDSGYIYKLYGFDY
                     IREFELLQEAGFHPLEVIRAATLHGAQALGLDQEIGTVEPGKLADLVVVAENPLQDFQ
                     VLYGTGTIRVNAGNEVVRVGGVRYTIKDGIVYDAPKLLADVRRIVAEAKKSSGRGIVQ
                     PGLESRPAGLPGGRPGDGHAH"
     gene            9907..13683
                     /locus_tag="VEL74_03290"
     CDS             9907..13683
                     /locus_tag="VEL74_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG61581.1"
                     /translation="MNQQQSAAALASSFKVTCPGCGQRLRFAVGPEAAGPDAAPRLRV
                     QCASCRATFSVRRPGAEPGAATTDGTMAAPPYVGVPVAPASTGSDRSVGSSGAASTAL
                     LSAAEAAPSLRRASRDAPGFVPGDLVADRYRVVRFIARGGMGEVYEADDLELRERVAL
                     KTVRAEVSRDDLVIERFKREIQLARKVTHPNVCRIFDVSHHREPREPDGAAIIFLTME
                     LLTGETLAQRIRDRGPMTPDEALPAASQIAEALHAAHQAGVIHRDLKPGNVVLVESRS
                     GLRAVVTDFGLARLEAGPEQGLTLTGTGVVGTPAYLAPEQIEGSEISPAVDIYALGIV
                     LYEMLTGTVPFLADTALATAVKRLREAPVSPRVHRPGLDPRWEAAILRCLERDPAARF
                     ADALQVVHALTGPARPMPLQPIPGPAPAAPAVEPSRAQPTTSRRRLQLLILTGLTLLV
                     AGVGYVRYTGWKEAQIAPRPGLLLPAAVTVRPSVAVLGFKDLSGNAETSWLSPALAEM
                     LSTELAAGGDLRVIAGDNVARAKVEMGLADVGQLERPALARLRTLLGSEAVVLGSYLV
                     LDGASGPQVRLDLLLQDAATGETTARLSESGPQSGLFTLVAKTGQRLRQALGAADPGS
                     PENAKPALPSHPEAARLYAEGLSKLRGFDPVGARDLLARAAVLEPGNAMVHSALATAW
                     GSLGYDNKAREEAKTAFDRSAGLPREERLLIQGRYREAVQDWNKAAEIYWQLWSMYPD
                     NLDHGLRLAGTQTAAGRVEEALATVDALRTLPAPFRDDPRIDLAEATAAGARADFQRQ
                     REAAARATRKGKEQEAPLMAAQALLLECRALRNLGQSDKAFAACEEGRALHARLGDRS
                     GVAEALLHAANVRFDRGDLASALRLSEEALVTYREIGNRAAEAGALNNVAVILKSQGD
                     LDRARELYEQVLAITREIGSRGGEAYALNNLAGVLLRRGDLERAGALFEESLGIRREQ
                     GDRSGEAYALDNIGVVLRKRGDLNGARRRHEEALRIRRQTRQKISEVTSLNNLAMVLL
                     DQGELEAARDRLRTSLALGREIGNPSAQAGALFGLGEVLAAEGRLAEARKHHETALEH
                     RHRLGEKGMEAESRLALALVLLQWGEAGAAAGLTTAAAGAFSHQGAIDGEALALSVSA
                     LALNTRGDAAGAGQALDRAAVLVARNQDLRTRLIVQLRAARLGRAPEGQSAETALRQV
                     SEQATRAGFPALRLEADVLLARQTRDAARLTTLEQEATAMGYGLLAREAADGLR"
     gene            complement(13675..14883)
                     /locus_tag="VEL74_03295"
     CDS             complement(13675..14883)
                     /locus_tag="VEL74_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYG61582.1"
                     /translation="MESPPPSFRARLGRNVLALSVVSLLTDVASEMTYPLLPVFLASV
                     LGAGATAVGAIEGAAESTAALLKLASGWWSDRVARRKPLVVAGYTIASVIRPLIGLAQ
                     SAAQVLAIRVTDRVGKGIRGAPRDALIADSVDPSIRGRAFGFHRAADHAGAVVGPLLA
                     FALLRWGGLDLRTLFLLTAIPGALAVAMLIFGVREVPRQAPVRNAIRPADLSVPLGRR
                     FWSFLVILLVFTLGNSTDAFLLLRAADLGVDAALIPILWAMLHVVKSAASTPGGALSD
                     RIGRKPLLVAGWLVYAGVYFALGQATREWHAWALFAVYGVYFGLTEGVEKALVADLVP
                     VERRGAAFGWYNLALGVGALPASLLFGALWDRWGAPAAFAFGAAMALMAAVGISAVAP
                     SRPSPARRSA"
BASE COUNT         2164 a         5512 c         5234 g         2107 t
ORIGIN      
        1 gtacgaactt tcttccagac ctattatgcg cccaataacg ccgttctcac cctcaccggt
       61 gatttcgacc cggaggacgc gctgcggcgg atcgagacct ggttcggcga catccccccg
      121 ggaccgccgg tgccgccggt cgtggccgag ctgcggccgc tgggcggcga gcggcgcgag
      181 gtgcgggagg acaacgtccg cctcccccgg atctatctcg gcttccgcgc ccccgccgtg
      241 ggcgagcgcc tctggtacgc cgccgacctg ctcgccggca tcctcaccgg cggcaagtcg
      301 gcgttgctct accgcgacct ggtctacgac cgccagatcg cccaggacgt gtcggcgtcg
      361 gtgagcccct atgaggaggt ggccaccttc ctggtggccg ccaccgcccg ccccggcgtg
      421 cgcgtcgaga cgctggaggc ggccctgctc gagcacctgg accggctggc cgcctccccc
      481 cccgcgcccg ccgacctcga gcgggcgcgc aaccggaccc tgatggacta tttcagcagc
      541 ttgcagaaga tcgagacccg ggccgaccag ttcgggatgt tcaccaccta tctcgacgac
      601 cccggcgccc tggcccgcga gtccgagatc ttcggcgagc tcaccggcca ggacgtccag
      661 gactacacgg ccggctggtg ccgcgagtcc gagcgggtgg tggtgacctt cgtgcccagg
      721 ggggaggcgt gatcgaccgc tcgctgccgc cgccccccgg cccgctccgt ccgttccgct
      781 tcccggcctt ccagcgggcg cgcctcgcca acggcctcac ggtcttcgcc gcgcgccagg
      841 ggagcgtgcc gctggtcagc ctggagctgg tcacccaggc cggcggccag cacagcccgg
      901 cgggccaggc cggcctcgcc tccctgaccg cctccctgct cgacgagggg acggcgcgcc
      961 gcacggcgct cgagatcgcc ggcgaggtcg agaacctcgg cggctatctg gtcaccggcg
     1021 ccgactggga cgtcggctac ctcggcgtcg gcctcctctc cagccatctg gagcgggggc
     1081 tggagctgct ggccgaggtg gccaccgccg cgaccttccc ggaagcggag atcgagcgcc
     1141 tgcggcggca gcggctgacc gagatcctgc gccgcaccca ggacccgtcg gcggtggccg
     1201 acgagcggct gctgcgggag atctaccacg gcacggtcta cgagggctcc ctgctcggca
     1261 ccgaggagag cgtgcgcggc ctcggccgcg acgccatcac cgccttcttc caccggcact
     1321 acgggatgga cggctcgttc gtcatcgccg tgggcgacct cgatcccggg gaggtcacgc
     1381 ggcgggtcga ggcggtgctg gggggggccg gcggcaccgc gccgccgccg cccgggatcc
     1441 gccccgcgcc gctcggcggg gtcgccgtcc acctggtcga ccgcccgggc gcggcgcagg
     1501 ccgagctgcg gctgggtcac ccctgcatct cgcgcacgca ccccgactac gtccctctga
     1561 tggtggccaa cgccctgctg ggcggcaagt tcaccagccg gatcaacctc aacctgcggg
     1621 agcggcacgg ctacacctac ggcgcctcga gccgcctcgt cggccgccag ggcccgggcc
     1681 ccttcctcgt cagcagcgcg gtggcgacgg agtcggccgg ggcggcggcg cgcgaggtgc
     1741 tgtcggagct gcgccggctg cgcgaggagc cggtcgaggt ggcggagctg gaggagaccc
     1801 tcagctatct caccggggtc ttcccctaca ccctgcagac gatcggcgag gtcgccaagc
     1861 gcctggagac cctgggcacc tacggcctgc cggacgacta ctacgagtcc tacctggagc
     1921 ggatcggcgc ggtgacgcgg gaggacatcc tggaggcggc gcgccggcac atccacccgg
     1981 agaggatcgc ggtggtggcg gtggggccgg ccgaggcgct ggagaggcag ttcgaaggat
     2041 tgggcccggt gaccgtctgg tcgccgcagg ccgagccgca gctcgcgggc taggactgct
     2101 cgccgacggc gtagagcctt cccgactggg tgatccggat gtcgatgcgg cggaccccgc
     2161 cgccgggtac gtcgacggtc accggctgct gggtccccgt cacctccaag tccttggggg
     2221 agtagtagac caggagcttg ttctctcccg cggggacctc gtaccccttg ctgaagtccc
     2281 ggctgcgccg gcgcttcagc agccccgcgc cgacgtcgaa gggctgatcc cagatcttcg
     2341 tggagcccag atagacgcgg acggaccctt cggcggccgg agcgtcgatg cgcaggctca
     2401 tctgcagccg gcccgtctgc ggcgcggccg acgaggacgt ctccgccccc gcgggcgttt
     2461 ccgccgccga gccttccgcg gactgcgccg gctgcggagt cagctctccg ccggccgcca
     2521 tctgggtgcc gcgctgccgg tcgacctcga tgcgcgcccg ccgcgcgatg tcggaggcct
     2581 ccctttccca cgggcgggcc tccagaacga ggctggcggc ggcgatggct tcggggtagc
     2641 ggcgcagacg ccactccgcc ttcgccttcg cgagatggcc ctcgaccgcc tcctcctggg
     2701 ccttcagccc gttgaattcg gcctccatcc tcaacgcctc ctggcgcagc cgggggatgt
     2761 ccggatggtt ccgggcgtgc ggctcggcga gcccgtacac cgaggcggct tcggagaagc
     2821 ggccccggcg catcagggcg ttcccctgct cgaacagctt ggcgacgtcg atggctgcct
     2881 cgggctgggg cgtcgccgcc accaccggcg tctcagccgc ctgccgggag tcgcgcgtcc
     2941 gggcgatccc ccagaagatg atgaacccga ggaacagggc ggcgatgacc gccccgatca
     3001 gctgggagct cctcttcctg gagctcccgg cggcggcagg ggggacggcc cgcggcggcg
     3061 ccggcatggg cagcggaggc ggcggcgtga aggagatcga ggagccggtg gccgacgcgg
     3121 ccgagccgtc gtcgttcgcc ggggtcatga ccgggggcgg cggcacggcc gccgggccgg
     3181 ccggggccgc cggccgggaa cccgatcccg tgccggtgcc ggaggtgccg gtgccggaga
     3241 ggaggtccgg agcggcgggc gggaccgcca ccaggggagg cggcggagcc acgggaatcg
     3301 gctccggggc cggcgcgccc ttgccgccac cgaagaggcg gccccacagc ggcttgcgcg
     3361 ccggcggggc ggccaccggt ccggcggctc cggccgatcc caccaatccc accgatcccg
     3421 gcgcgggccc gaccgtcgac ggcgaagccg tcgaggatgc tcccaggggg agcgagccgg
     3481 acgggatcac catggtggac gacaggctct gcgtctcgtt gagctcgtcg ccgttcaggc
     3541 tctccgcgac cagcttcttg agctcgtcgg ccatctcccg cgcccgctgg tagcgccggt
     3601 tcgggtcctt ctccagcgcc ttggagatga tcttctccac tccgggcggc acgttgcggg
     3661 cgtactcccg cagcggggtg aactggtcgt agacgatgcg gtggctcacc accgtcaggt
     3721 tctcgccctg gaacggcttg tggcgcgtca gcatctcgta cagcaccacc ccgagggcga
     3781 agatgtccgc ccggtggtcc acctctttcc cctgcacctg ctcgggcgcc atgtagttgg
     3841 gcgtgccgag gagctgcccc tcctgggtga ggttcgaggt gtcgaggcgg gcgatgccga
     3901 agtcggtgat cttgaccctg ccgtcctcgg tcatgatgat gttcgccggc ttgacgtcgc
     3961 ggtggatcac ccgctgcgag tgggcgtagt ccagcgcctc ggccacctgg gagatgatgt
     4021 ccgccacgcg gccgaggggc agcgaggggt ccgcctgcat caccatcttg aggttggtcc
     4081 cccgcacgta ctccatggcg atgaaggaca ggccgtcgcc ggccccctcc accacgtcgt
     4141 ggatggtgac gatgttgggg tgcgagagga tgccggcgct ctgcgcctcg cggatgaagc
     4201 gggcgcggaa ctggtccgcc tcctggtcgc gcaccgagaa gcccatgcgg aaggtcttca
     4261 aggcgacgag ccggccgatc agcgggtcgc gggccaggta gaccacaccc atggcgcctt
     4321 tcccgatctc gtcgaggatc tcgtaacggc ctagcttggt cggcgtcagg ggcaacatgg
     4381 ctactcgctt aagtgcatgg cgattctagg gctccggcgc cgccgagtca aaccgccggg
     4441 gaacccggcg gcggaacggg taactcgtgg ggacgtatgg gcttaggggc gccgggccgg
     4501 cgccccctcc tgtggtaaga tgcgacccct tgacttttgc cctctgaagg aggcttgaga
     4561 cgatgcaggt ggacagcccg gagaagatcc gcaatatcgc ggtggccggt cacaacgaca
     4621 ccggcaaaac gactctcgtc agcgcgctcc tctacgccgg tggcgtcacc acccggctcc
     4681 atcgtgtcga ggacggcaat acgctgaccg acttcgacca cgaggagacc gagcgcggca
     4741 tctcgatcgg gctcgcgccc tgcttcgttc cctggcaaca gaccaagatc aacctcatcg
     4801 actgccccgg ctacggcatc ttcttcaccg aggcgaaggc gggcatgcgc gccgccgacg
     4861 ccgtgctgct ctgcgtcaac tccgtggccg gcgtcgaggt caacaccgag aagatctggt
     4921 cgtacgccga ggagatcgac ctgccggtga tgatccacct caccaagatg gaccgcgagc
     4981 gggccgactt cgaccgcgcc ctcgaggggc tccacaagag cctcggccgg ctggtgatcc
     5041 cggtgcagat cccgatcggc cgcgagcagg gcttcaccgg cgtcgtcgac ctggtgcggc
     5101 agaaggccta caagttcacc cgcgacggca acggcaaggc ggaggccgcc gacatcccgg
     5161 ccgacctggc gggcgaggtg gaggcccacc gcgcgcgcct gatggaggcg gtggcggagg
     5221 gggacgaggc gctgatggag cgcttcttcg aggaggggac gctgccccag gaggacctcg
     5281 agcgcggcct gcgcctggcg gtccgcaacc ggcaggtctt ccccctgacc gtctccgccg
     5341 gctcgcacgg catcggcccc tcggcactgc tcgactcctt cgtcgccctg ctcccctccc
     5401 ccgccgaccg gaagcccttc ccggcgcaga ccgtcggcgg cgaggacctg gaggtcggca
     5461 ccgacccggc ggggccgctg gccgccctgg tgttcaagac gctgtcggac ccgttcaccg
     5521 gcaagatctc catcttccgg gtcgtctccg gcaccctctc ccaggattcg aacgcctaca
     5581 actgccgcgc cgaggagaac gagcgcatcg gccacaccat gctgctccaa ggcaagcagg
     5641 tcaccaacgt gccgcgcctg gtggccggcg acatcggcgg cctggccaag ctcaaggtca
     5701 cctcgacggg cgacaccctg tgcgccaagg agcgcccggt gcgcctgggc tggatcacgg
     5761 tgccggaggc cgccatggcc ttcgccgtcg agcccaagtc caagggggac gaggagaaga
     5821 tcggcgacgc cctgcaccgg ctgatggacg aggacgtgtc gctccacacc ggccgcgacc
     5881 ccgagacgca cgagtacctg ctctccggca ccggccagct ccacatcgag atcgccgtct
     5941 ccaagctcaa gcaccgctat cacgtcgagg tggttctgca tccgccgaag gtgccgtacc
     6001 gcgagacgat cctgcggccc gccgacggcc acggccgcca caagaagcag agcggcggcc
     6061 gcggccagtt cgccgactgc aagatcaaga tcgagccgct gccgcgcggc gaggacttct
     6121 ccttcgccga cgagatcttc ggcggctcga tcccgcagaa ctaccggccg gcggtcgaga
     6181 aggggatcca ggaggcgcgg cgccgcggct acctggcggg ctatccggtg gtcgacttca
     6241 aggtgcgcct cctcgacggc cagtaccacg acgtcgactc ctccgagctg gcattcaaga
     6301 tcgccggctc cctggccttc aaggacgcca tggccaacgc caagccgacg atcctcgagc
     6361 cgatcatgaa ggtggaggtg gtcgcctccg acgagttcac cggcgacatc atgggcgacc
     6421 tgtcgcaccg ccgcggccgg ccgcagggca tggactcgat gaacggcaac caggtcatca
     6481 aggccacggt gccgatgtcc gagatgctca actacgcgcc ggctctgcgc tccatgaccc
     6541 agggacgggc gaccttccac atggagttct cccactacga ggaggtcccc cggccggtcc
     6601 aggagaagct gatcgccgag gcggccaagc accggcagga agagcagcag catcactaga
     6661 cccgctacaa tctgaagcct ccggggcggc gggatcccgc cgccccgatc catcagacac
     6721 cacgacaaca caccgtcacg gagatccagc cttgatccaa gcgacccaga tgcgcgtcgg
     6781 catgtgcatc ctcttcgagg gcaacatctg ccggatcatg caagtccagc acgtcacgcc
     6841 ggggaactgg cgcggcttcg tccaggcccg gatgcggaac atcaagtccg gcaacagctt
     6901 cgagcaccgc ttcggctcca ccgagaaggt agagcgggcg atcctcgaca ccagtccgat
     6961 ggagtacctc tactccgacc cgtcgggcca ccacttcatg aaccaggaga cctacgagca
     7021 gatcaccatg gaggacgaga ccctgggtga caccatgctc tacctcctgc ccaacaccgt
     7081 catctcggtg gacttctatg aaggccagcc ggtgggcatc gacctgccga acacggtgac
     7141 gctggaggtg gtggagaccg agcccggcct caagggggcg acggcgagct cctcgtacaa
     7201 gccggccaag atggagaccg gcctgacggt ccaggtgccg cccttcatcg aggtggggac
     7261 caagatcctc atcgacaccc gcgagaacaa gtacctgagc cgcgcgtgag ccccgaacag
     7321 ctcgatcaga tcgtcctggg cctcacctgg ttcgtggtct tcctgttctc gaccacgctc
     7381 cacgaggccg gccacgcctt cgccgcctac cggctgggag actccaccgc ctacgagggc
     7441 ggccaggtga gcctcaaccc gctcccgcac atccgccggg agccgttcgg catggtgctg
     7501 gtccccctca tcaccttcgc caccatgggc tggatgatgg gctgggcgag cgccccctac
     7561 gaccccacct gggcccaccg ctacccgcgg cgggcggcgc tgatggccct cgccggcccg
     7621 ctcggcaacc tgatcctggt gctggtggcc gggctggcgc tgcgcgccgg gctggccatg
     7681 ggggtgttcg tgccggctcc ccaggacttc ttcacctacc tggtgagccc ggcgggggac
     7741 agcgccgcgg caggcgccat cgtgccgctg tcgatcctgt tctcgctcaa cctcctgctc
     7801 ttcgtcttca acctgctgcc gctgccgccc ctcgacggca gcgcgatcgt ccctgccttc
     7861 atgagcaaca cgatggtggc gcgctacaac gccttcctcc accagccgat gctctccctc
     7921 ctgggcctgc tggtggcctg gaaggtcttc cccttcatct tccagccggt cctgatcctg
     7981 gccctgcggc tgctgtactc ggggctcggc tgacgcctag tgcgcgtggc cgtcgcccgg
     8041 ccggccaccg ggcaggccgg ccggccggct ctccaggccc ggctggacga tccccctgcc
     8101 ggacgacttc ttggcctcgg cgacgatccg ccgcacgtcg gccaggagtt tcggcgcgtc
     8161 atagacgatg ccgtctttga tcgtgtagcg gacgccgccc acccgcacca cctcgttgcc
     8221 ggcgttgacc cggatggtgc cggtgccgta gagcacctgg aagtcctgga gcgggttctc
     8281 cgccaccacg acgaggtcgg cgagcttgcc gggctccacg gtgccgatct cctggtcgag
     8341 ccccagcgcc tgcgcgccgt gcagggtggc cgcccggatc acctccaggg gatggaaccc
     8401 ggcctcctgc agcagctcga actcgcggat gtagtcgaag ccgtagagct tgtagatgta
     8461 gccgctgtcg gagccggtgg tcacccggcc gccgcggttc ttgtactcgt tgaggaaggt
     8521 catccagagc cggtagttgt tgcgccaggc cacctcgtcg ccggtggtcc agttgtacca
     8581 gtaggagccg tgggccttgc ggctgggggt gtagaaatcc cagagcgacg gcagggtgta
     8641 ctcctcgtgc cactcggcgc ggcgggcgcg catcaggtcg cggctcgcct cgtagatggt
     8701 gagcgtcggg tcgagggtga agtcgagggc gatgagctcg tccatcacct tgttccagcg
     8761 ctcggagaag gggggagcgg cctgctccca cagccggccg gcctcgccga agcggtgcga
     8821 ctcgtcgagg aagttgtagt cggacggata gtcctggatg ctgcggtcgc cgaacagcgc
     8881 ctcgggcagg ccgtaccagt gctccatggt ggtgaggccc cagcgcgcgg tgtccaggac
     8941 gtcggcctgg gcgacgtaga gctgggcgtg atgggcggcg gtgcgcaggc cccgcttctt
     9001 cgcctcctcg atggcggcct tcaacatctc cggccgctcg ccgaagagct tgatgccgtc
     9061 ggcgccccgg gtggcgaggc ccgccaccca ctggcgcacc tcgtccggag tcttgaacgg
     9121 ggtgtcgcgg tccataccga agacgacgta ggactcgatg cggggcgcgg tgatctcgtt
     9181 gcgggcgctc ttcgccttct cgtccagggt ccagtcgatg ccgttggtgc tgccgggctc
     9241 gcggaccgtg gtgatgccgt gccccatcca gagcttgtag acgtattcgg ccgtggtgcc
     9301 ctgggcgcgg ccgccggtgt ggccgtgcat gtcgacgaag ccgggcagca cgtacatccc
     9361 ctggaggtcc agctcgcgcc ccccctcccc cagcagcggg cggctctggg gatcgatggg
     9421 cacgccggga tagcccaccg gcacgatgtc ggcgatccgg ttcttctcga tcacgatgtc
     9481 gaccggcccc agcggcggag cgcccgtgcc gtcgatcagg gtgaccccgc gcaggatcag
     9541 gcggccgtac ggcccctcgc cctcggcgcg cggcggcgcc ttgggcacct cctggggggc
     9601 ccaggccgcg ggcggcggcg gagagggcgg cggggtctgg ggagcggcgg ccggggcctc
     9661 cgctttccgg gtgttctgct cctgcttctc ctgggctccc accgccaggc agcaggcggc
     9721 gatcacggcg gtcaacgcga ctgcggtacg gccaacccac gggtctcgtc tggtcttcat
     9781 cgatccggct cctcgctcga gtccaagggc gcatcctgac aaaagcggcc cggcgcggca
     9841 aggtggtaag atgaggcaaa ttcagccacg ttacccgtcc aggctccgcc gggaccagac
     9901 attcggatga accagcagca gtctgccgcg gcgctcgcct cgtccttcaa ggtcacctgc
     9961 ccgggatgcg ggcagcggct gcgcttcgcc gtcggcccag aggcggccgg tcccgacgcc
    10021 gcgccccggc tgcgggtgca gtgcgcctcc tgccgcgcca ccttcagcgt gcgccgcccc
    10081 ggggccgagc cgggcgcggc caccactgac ggcaccatgg ccgcgcctcc ctatgtcggc
    10141 gtgcccgtcg cgccggcgtc gaccggctcc gaccgatccg tcggatcttc cggcgccgcc
    10201 tccaccgcac tgctgtccgc ggccgaggcc gcgccgtccc tccgccgggc ctcccgggac
    10261 gctcccggct tcgtgccggg ggacctcgtc gccgaccgtt accgcgtggt gcgcttcatc
    10321 gcccgcggcg gcatggggga ggtctacgag gccgacgacc tggagctccg ggaacgcgtg
    10381 gccctgaaga cggtccgcgc cgaggtctcg cgggacgacc tggtgatcga gcgcttcaag
    10441 cgggagatcc agctcgcgcg caaggtcacc caccccaacg tctgcaggat cttcgatgtc
    10501 tctcaccacc gggagccgag ggagccggac ggcgccgcca tcatcttcct gaccatggag
    10561 ctcctcaccg gcgagaccct ggcgcagcgc atccgcgacc ggggtccgat gacgccggac
    10621 gaggcgctcc cggccgccag ccagatcgcc gaggcgctcc acgccgccca tcaggccgga
    10681 gtgatccacc gcgacctgaa gccgggcaac gtcgtgctgg tggagtcccg gagcgggctg
    10741 cgcgccgtgg tcaccgattt cgggctggcg cgtctggagg ccggtcccga gcaggggctg
    10801 acgctgaccg ggaccggcgt cgtcggcacc cccgcctatc tcgcccccga gcagatcgag
    10861 gggagcgaga tcagcccggc ggtggacatc tacgcgctgg gcatcgtgct ctacgagatg
    10921 ctcaccggca ccgtgccgtt cctcgccgac accgcgctgg cgacggcggt caagcgcctg
    10981 cgcgaggcgc cggtctcgcc gcgggtccac aggccgggcc tcgacccgcg ctgggaggcc
    11041 gccatcctgc gctgcctgga gcgcgacccc gccgcccgct tcgccgacgc cctgcaggta
    11101 gtgcacgcgc tgaccggccc ggcgcgcccc atgccgctcc agcccatacc cgggccggcc
    11161 cctgccgctc ctgccgtcga gccctcacga gcccagccca cgacgagccg aaggcggctc
    11221 cagctcctga tcctgaccgg actgaccctg ctggtggccg gggtgggcta cgtccggtac
    11281 accggctgga aggaggccca gatcgcgccg cggccggggc tcctgctccc cgccgcggtc
    11341 acggtccgcc cgtcggtggc ggtcctcggc ttcaaggacc tgtccggcaa cgccgagacc
    11401 tcctggctct cgccggccct cgcggagatg ctgtccaccg agctcgcggc cggcggcgat
    11461 ctgcgggtga tcgccggcga caacgtggcc cgggccaagg tcgagatggg gctcgcggac
    11521 gtcggccagc tcgagcggcc cgccctcgcc cgcctccgca ccttgctggg gagcgaggcg
    11581 gtggtgctgg gctcttacct ggtgctggac ggcgcctcgg ggccgcaggt ccgcctcgac
    11641 ctcttgctgc aagatgccgc caccggcgag accacggcgc ggctgtcgga gtccggcccc
    11701 cagtccgggc tgttcaccct ggtcgcgaag accggccagc gcctgcgcca agccctcggg
    11761 gcggccgatc ccggctcacc ggagaacgcc aagccggcgc tcccctccca ccccgaggcg
    11821 gcccgcctct atgccgaggg gctcagcaaa ctgcgcggct tcgatcccgt cggtgcccgc
    11881 gacctgctgg cacgtgctgc cgtcctcgag cccggcaacg cgatggtcca ctccgctctc
    11941 gccaccgcct gggggtccct gggctacgac aacaaggccc gcgaggaggc gaagacggcc
    12001 ttcgaccgct ccgccggcct gccgagagaa gagcggctcc tcatccaggg gcgctaccgc
    12061 gaggccgtgc aggactggaa caaggcggcg gagatctact ggcagctctg gtccatgtac
    12121 cccgacaacc tcgaccacgg cctccggctc gccggcaccc agaccgccgc cggccgggtg
    12181 gaagaggcgc tggccacggt cgacgccctc cgcaccctgc ccgcgccgtt ccgcgacgat
    12241 ccccgtatcg acctggccga ggccacggcg gccggcgccc gcgccgactt ccagcggcag
    12301 cgcgaggcgg cggcccgcgc cacccgcaag ggcaaggagc aggaggcgcc gctcatggcc
    12361 gcccaggccc tcctcctgga gtgccgggcg ttgcgcaacc tggggcagtc cgacaaggcc
    12421 ttcgccgcct gcgaggaggg gcgcgccctg cacgccaggc tgggagaccg ctctggcgtg
    12481 gccgaggccc tcctccacgc cgccaacgtc cgcttcgacc gcggcgacct ggcatcggcg
    12541 ctgcggctgt ccgaggaggc cctggtcacc taccgcgaga tcggcaaccg tgccgccgag
    12601 gccggcgccc tcaacaatgt cgcggtgatc ctcaagagcc agggggacct ggaccgcgcg
    12661 cgcgagctgt acgagcaggt tctcgccatc acccgcgaga tcggcagccg cggcggcgaa
    12721 gcctatgctc tcaacaacct ggccggcgtc ctgctgcggc gcggcgacct ggagcgcgcc
    12781 ggcgccctct tcgaggagtc cctcggcatc cgccgcgagc agggagaccg gagcggcgag
    12841 gcctacgccc tggacaacat cggtgtggtt ctgcgcaagc gcggcgacct gaacggggcg
    12901 cgccggcgcc acgaggaggc gctccgcatc cgccgccaga cccgccagaa gatcagcgag
    12961 gtgacctccc tcaacaatct ggccatggtg cttctggacc agggagagct ggaggcggcc
    13021 cgcgaccgcc tcaggacctc cctcgccctg ggccgggaga tcggcaaccc cagcgcccag
    13081 gccggcgccc tcttcggctt gggagaggtg ctcgccgccg agggacgcct ggcggaggcg
    13141 cggaagcatc acgagacggc cctcgagcac cgccaccggc tgggcgagaa gggcatggag
    13201 gccgagagcc gtctggccct cgctctggtg ctcctgcaat ggggcgaggc cggcgccgcg
    13261 gctggactca ccaccgccgc cgccggggca ttctcccacc agggcgcgat cgatggcgaa
    13321 gccctcgccc tctcggtctc ggccctggcc ctgaacaccc ggggcgatgc cgccggggcg
    13381 gggcaggctc tggaccgggc cgccgtcctc gtcgcccgca atcaggacct ccgcacgcgc
    13441 ctgatcgtcc agctccgggc ggcccgtctg ggccgcgcgc ctgaggggca gagcgcggaa
    13501 accgcgctcc gtcaggtctc tgaacaggcc acccgcgccg gcttccctgc tctccgtctg
    13561 gaggcggacg tgctcctcgc ccgtcagacc cgggacgcgg cgcgtctcac caccctcgag
    13621 caggaagcga cagccatggg ctatggtctc ctcgcccgcg aggctgccga cgggttacgc
    13681 tgaccgccgg gcaggcgagg gccgagaggg cgccacggcg gagattccca ccgcggccat
    13741 cagcgccatc gccgcgccga aggcgaaggc cgcgggcgcc ccccagcggt cccacagcgc
    13801 gccgaagagc agcgacgccg gcagggcccc gacacccagg gcgaggttgt accagccgaa
    13861 ggcggccccc cgccgctcca ccggcacgag gtcggccacc agcgccttct ccaccccctc
    13921 ggtgagcccg aagtagaccc cgtagactgc gaagagcgcc cacgcgtgcc actcccgggt
    13981 ggcctgcccg agagcgaagt agacgccggc atagacgagc cagccggcca ccagcagcgg
    14041 cttgcggccg atccggtcgg agagcgcccc gccgggcgtg ctcgccgccg acttgacgac
    14101 gtggagcatg gcccagagga tcgggatcaa cgccgcgtcc acccccaggt cggccgcgcg
    14161 cagcaacagg aaggcgtccg tcgagttgcc cagggtgaag accaggagga tcaccaggaa
    14221 ggaccagaag cgacggccca gaggcaccga cagatcggcg ggccggatgg cgttgcggac
    14281 gggcgcctgt cgtggcacct cacggacgcc gaagatgagc atcgccacgg ccagcgcccc
    14341 ggggatggcg gtgagcagga acagcgtgcg caggtcgagc cccccccagc gcagcagggc
    14401 gaaggcgagg agaggcccca ccaccgctcc ggcgtggtcg gcggcgcggt ggaagccgaa
    14461 ggcccggccg cggatcgaag ggtccaccga gtcggcgatc agcgcgtccc gaggggcacc
    14521 gcggatcccc tttcccaccc ggtccgtcac ccggatggcg agcacctggg ccgccgactg
    14581 ggcgaggccg atcagcggcc ggatcaccga ggcgatggta tagccggcga cgaccagcgg
    14641 cttgcgccgg gcgacgcggt ccgaccacca gccgctggcg agcttgagca gcgccgccgt
    14701 ggactcggcc gccccttcga tcgcccccac cgccgtcgcc cccgctccca gcacgctggc
    14761 caggaacacg gggagcagcg gataggtcat ctcggacgct acgtcggtca gcaggctgac
    14821 gacagagaga gcgaggacat tgcggccgag ccgcgcgcgg aaagaggggg gcggggattc
    14881 caccggcgga ttctacctcc ccaaaaagcg aacggccggc gcgagatgcg ccggccgtca
    14941 gccgttctgc aatggccggg aaacctactc cgcggcctcg gcgtccttct tcttggcgcg
    15001 gcggggcttg gcagctt
//
LOCUS       DASPJI010000119         9938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_752014, whole genome shotgun sequence.
ACCESSION   DASPJI010000119 DASPJI010000000
VERSION     DASPJI010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9938)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9938
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752014"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VEL74_03300"
     CDS             <1..346
                     /locus_tag="VEL74_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61583.1"
                     /translation="QVREAIGRHIALLQKGEVDAQRLARAKSYLRNSFLLSLSTPGSV
                     ARQVAQVIALTGKVEDLDRTYALYEKVTPAEVQRLTRDVFRSDRETVVTLAQAGGNAA
                     AKPAAGSQGGAQ"
     gene            343..1857
                     /locus_tag="VEL74_03305"
     CDS             343..1857
                     /locus_tag="VEL74_03305"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYG61584.1"
                     /translation="MTDRRFAVAPLLAVSLALGAAAASAQAAPAGAIETVTLPSAGSP
                     LVSIQLMFDAGSIHDPKGKEGLASLTAAMVGQGGTRKRSYSELLESLYPMAAAISSGA
                     DREVTVISGTVTRESLADYTALFTEALLQPAFSEADFTRNREQALTYLTNTLRSADDE
                     LLGLEAIQGEIFTGHPYSHPAAGTVEGLKSITLDDVKRFYQEHYTRANLMLGVAGGYP
                     DGYVAGLQKTLAALPAGKPGRKELPAPRKVEGRRFVLIEKQTGSVGVHLGYPLPINRS
                     HPDFYPLMVANSYLGEHRTSHGTLMQKLREQRGLNYGDYSYIEYWPAPPVTTHPGPGV
                     PRRQQYFSVWLRPVVPGDAQFALRAALHEVGRLHDEGMTKEEFELTRDFVVSYSKLWA
                     QSLSTRLGILMDSKYYGMPYWIDEIEARLSKLTVDDVNRAIRKYIHTDDYVAVMVTAN
                     AAALKETLQKDAPSPKAYNTPPAEAITAEDKKLVGLKVAPTSIEIVPVEQMFQK"
     gene            complement(1862..3601)
                     /locus_tag="VEL74_03310"
     CDS             complement(1862..3601)
                     /locus_tag="VEL74_03310"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HYG61585.1"
                     /translation="MKRTRSPRELWASLLPTAGALLVLAVASRFAALDGTGVPSPTLL
                     LVMVFAGTVGACHRPLAGGPATPGGPVTQGLGTAVLPPMLLLGGVFPAVWLGAGTFLI
                     AELSLRLARRLFALQPVERRGLPRALESTGRAALAILAAGVVWSWLRSDRGLPVAWTG
                     AAAAYLGVWIGLELADRKIRRPELPLRLRLILPPFAIDAAGWAVGGALALAGRAAGWR
                     LGGILLAVFALLVLEAVRNWMLLERAQRRVRDLERLRRAGKRMFFAAQEMAAVVERIR
                     VECAKVVPFLWFQFEALAPGSEFKSWWAGPQGVLQEGVPEPDRHPPALPGVHRRAAWQ
                     IIDHHLRVDGKVLARLQLWCDPRRLNAESIELLDHLLPQMSHSMQRCLLDREAREDPL
                     TGLAMRRVLEKQLHEVHARCHEEGGAMAVVLCDLDHFKRINDTWGHPAGDAALVAFAG
                     LLKETRRPADLCCRYGGEEFVLLLDRTTGTEALELAEGLRRRVEELVFEVEGQRVPLT
                     VSAGVASFPGLFVRGAAELILFADEALYEAKRMGRNRCLLDLGQGRYQDVEGNVHETE
                     EAPPVVEPPRIFA"
     gene            complement(3683..4687)
                     /locus_tag="VEL74_03315"
     CDS             complement(3683..4687)
                     /locus_tag="VEL74_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HYG61586.1"
                     /translation="MSPPPFPLPSRRLEIPGFAIGHGSDRAGMTGVTVVLCPQGMMAA
                     AEVRGSATGTRQFDSLVSGIHVGSRAHAVVLAGGSAFGLAAADPVVDWLARRGHGFQT
                     GVAVVPQVPTAILFDLALGDKTARPGTALVEAALESAASAASGEVPVGSVGAGTGATV
                     GKALGPAQGMKGGFGFASLTVPGGVTVAAAVAVNAFGDVRRLDGRLLAGCRAAPGSLE
                     LAGAERVLAALPPDTPHPWEGNTTLAVVMTDAVLTKATALKVCQMAFGGFYRTLAPAL
                     SLFDGDLVVTLASGARPAHVNQVGVLAERAVAEAILTAVLEADGFGLLPAARDLPGAG
                     "
     gene            4773..5240
                     /locus_tag="VEL74_03320"
     CDS             4773..5240
                     /locus_tag="VEL74_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61587.1"
                     /translation="MQDRSSDPSAVVWNWRKPGSDDGDARSREAAAARKRGLLGLAVG
                     LAVAALIYFFWKPVLAAVVAGIALVMGLIALAAPLTLYKKVTRALDLFAHGVGMTVTW
                     VLMTLLYYLLFLPVGAVLRARRRLGITRSFDPALSTYWKTTGERAPESYRKQF"
     gene            5242..7092
                     /locus_tag="VEL74_03325"
     CDS             5242..7092
                     /locus_tag="VEL74_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HYG61588.1"
                     /translation="MKLLGISCYYHDSAACLIDEGRIVAAAQEERFTRKKHDAGFPYN
                     AVRYCLAAGGIGEGGGELGGVAFYDKPLLKFHRLLETYLAVVPKGLRSYVMAVPLWLR
                     EKLWIPPHILEALESCGVGGDGRSPDIFFTEHHESHAASAYYPSPFAEAAVLTLDGVG
                     EWATTTIGVGEGHRLRLIRQIDFPHSLGLLYSAFTHFTGFKVNSGEYKLMGLAPYGEG
                     KYTDLIKRELIDVREDGSFRLNMDYFGYLDGLEMTNERFAALFGGPPRKPESPIGKRE
                     MDLARSIQEVTEEIVLQLARQARELTGKRHLCLAGGVALNCVANGILLRAGIFDDLWI
                     QPAAGDAGGALGAPLAVWHNAMNKPRVTVSGGRDAMRGAFLGPEFPGPRIKEFLDEHG
                     YVYEELADADWAPRIAALLAGENVVGLCHGRMEFGPRALGNRSIIGDPRSPRMQSIMN
                     LKIKYRESFRPFAPSVLVERVSDYFELDRPSPYMLLVADVVEGRRIPKRGDEAGLPLV
                     EWVNRPRSDVPAITHVDYSARIQTVDREDNPRYHALISEFERLTGYGVIVNTSFNVRG
                     EPIICTPEDAYRCFMRTEMDYLVLGPFLLDKKAQPVFEEGRDWRQDYQLD"
     gene            7122..7286
                     /locus_tag="VEL74_03330"
     CDS             7122..7286
                     /locus_tag="VEL74_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HYG61589.1"
                     /translation="MRRLGYIGSLLGEIVQFARQNKAYWIVPFVLILALVVLLVVTSQ
                     ATAPFIYTLF"
     gene            7323..7799
                     /locus_tag="VEL74_03335"
     CDS             7323..7799
                     /locus_tag="VEL74_03335"
                     /inference="COORDINATES: protein motif:HMM:NF021587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing protein"
                     /protein_id="HYG61590.1"
                     /translation="MRRFKPVHGLFLVVLFVGAVLAADYALEGRFGGGGFRRVGPDKN
                     GVVRIDVSDLQPRQVRFYRFLNYGNQEVKFLVGRDAEGQVQVGFDASETHGAAGRGFK
                     HDGDWLVDKKCETTSRLAEVNSGRGGCRPVPLKHQIVGDELVLAENDILLGWRYFR"
     gene            7902..8396
                     /locus_tag="VEL74_03340"
     CDS             7902..8396
                     /locus_tag="VEL74_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HYG61591.1"
                     /translation="MSSADFVLYEEEFKHLEEALRRLRQEANARAVFLIDKNGQQIAS
                     AGEIEQFDTTSLASLTAGNVAATDGLAKLIGEREFSVLFHEGQQDHIHISIVAKRAIL
                     VVIFDDRSSLGLVRLRVKRASVDLEKIFDVMVQKAERGAQGPAHASPFSEITDDDIDA
                     LFGE"
     gene            8514..9098
                     /locus_tag="VEL74_03345"
     CDS             8514..9098
                     /locus_tag="VEL74_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HYG61592.1"
                     /translation="MTFINYASKEINCKIVYYGPGLCGKTTNLQYIYNKTAPESKGKM
                     ISLATEADRTLFFDFLPLDLGSIRGFTTRFHLYTVPGQVFYDASRKLILKGVDGVVFV
                     ADSQEERMEANIESIRNLEANLKEHGFDLSTIPYALQFNKRDLPNVVAVEEMYRTLNY
                     KREPTFEAVATTGVGVFDTLKSVAKQILIELRKR"
     gene            9134..9637
                     /locus_tag="VEL74_03350"
     CDS             9134..9637
                     /locus_tag="VEL74_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG61593.1"
                     /translation="MNTPNAAPASADALDIYTAGIAALGRQDWAEAVRLFETVIQESD
                     TPELTGRARQFLATSRQKLARNQQEDRPTGDDDPYLRALFEKNRGDFRQALELCRQDG
                     REQKDDRFAYLAASIHALEGRPDDAVQTLTRAVELNPKNRIHAFHDADFADLRRNRDL
                     RPLFGLS"
     gene            9651..9938
                     /locus_tag="VEL74_03355"
                     /pseudo
     CDS             9651..9938
                     /locus_tag="VEL74_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017310954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
BASE COUNT         1576 a         3610 c         3379 g         1373 t
ORIGIN      
        1 ccaggtgcgg gaggcgatcg gccgccatat cgccctgctc cagaaggggg aggtggacgc
       61 gcagcggctg gcgcgggcca agtcctacct gcgcaactcc ttcctgctct ccctgtccac
      121 cccgggctcg gtggcgcggc aggtggcgca ggtcatcgcc ctcaccggca aggtcgagga
      181 cctcgaccgg acgtacgcgc tctacgagaa ggtgacgccg gcggaggtcc agcggctcac
      241 ccgcgacgtc ttccgtagcg acagggagac cgtcgtcacc ctcgcccaag ccggcggcaa
      301 cgccgccgcg aagcccgcgg cgggctctca gggaggtgcc cagtgaccga tcgcagattc
      361 gccgtggcgc ctctcctcgc cgtcagcctg gcgctcgggg cggccgccgc ctccgcgcag
      421 gcggccccgg ccggggccat cgagaccgtg acgctcccca gcgccggctc gccgctggtg
      481 tcgatccagc tgatgttcga cgccggctcc atccacgacc cgaagggcaa ggaggggctg
      541 gccagcctga ccgccgccat ggtgggacag ggcggcaccc gcaagcgctc ctacagcgag
      601 ctgctggagt ccctctaccc catggcggcc gccatcagct ccggcgccga ccgcgaggtg
      661 acggtcatct ccggcacggt gacccgggag agcctggccg actacacggc gctgttcacc
      721 gaggcgctcc tccagcccgc cttctcggag gccgacttca cccgcaaccg ggagcaggcc
      781 ctcacctatc tcaccaacac cctgcgcagc gccgacgacg agctcctcgg cctcgaggcg
      841 atccagggcg agatcttcac cggccacccg tacagccacc cggccgccgg cacggtggag
      901 gggctcaaga gcatcacctt ggacgacgtc aagcgcttct atcaggagca ctacacccgg
      961 gccaacctga tgctgggcgt cgccggcggc tatcccgatg gctacgtcgc cggcctgcag
     1021 aagacgctgg cggcgttacc tgccggcaag cccgggcgca aggagctgcc ggcgccgcgc
     1081 aaggtggagg ggcgccgctt cgtgctgatc gagaagcaga cgggctccgt cggcgtccat
     1141 ctcggctacc cgctgcccat caaccggtcg cacccggact tctacccgct gatggtggcc
     1201 aacagctacc tgggcgagca ccggacctcc cacggcacgc tgatgcagaa gctgcgcgag
     1261 cagcgcggcc tcaactacgg cgactactcc tacatcgaat actggcccgc cccgccggtc
     1321 accacccacc ccggaccggg ggtgccgcgg cggcagcagt acttctccgt ctggctgcgg
     1381 ccggtggtgc cgggcgacgc ccagttcgcc ctgcgcgccg ccctgcacga ggtcgggcgc
     1441 ctgcacgatg aggggatgac gaaggaagag ttcgagctga cccgcgactt cgtggtctcc
     1501 tactcgaagc tctgggcgca gagcctgtcg acccggctgg gcatcctcat ggacagcaag
     1561 tattacggca tgccgtactg gatcgacgag atcgaggcgc ggctgtcgaa gctcacggtc
     1621 gacgacgtca accgggccat ccgcaaatac atccacaccg acgactacgt ggcggtgatg
     1681 gtcacggcca acgccgcggc gctcaaggag accctgcaga aggacgcgcc gagcccgaag
     1741 gcctacaaca ccccgccggc cgaggccatc acggccgagg acaagaagct cgtgggcctg
     1801 aaggtggcac cgacctccat cgagatcgtg ccggtggagc agatgttcca gaagtaaaag
     1861 gtcaggcgaa gatccggggg ggctccacca cgggtggggc ctcctcggtc tcgtggacgt
     1921 tcccctccac atcctggtag cgcccctgcc cgaggtcgag caggcagcgg ttgcggccca
     1981 tacgcttggc ctcgtagagg gcctcgtcgg cgaacaggat cagctccgcg gcgccgcgca
     2041 cgaagaggcc ggggaacgag gcgacgccgg cgctcaccgt cagcggcacc cgctgcccct
     2101 ccacctcgaa caccagctcc tccacccggc gccgcagccc ctcggccagt tccagggcct
     2161 ccgtgcccgt ggtccggtcg agcagcagga cgaactcctc gccgccgtag cggcagcaga
     2221 ggtcggcggg ccggcgcgtc tccttgagca ggccggcgaa ggccaccagc gccgcgtccc
     2281 ccgccggatg cccccaggtg tcgttgatcc gcttgaagtg gtcgaggtcg cagagcacca
     2341 ccgccatggc ccccccctcc tcgtggcagc gggcgtgcac ctcgtggagc tgcttctcca
     2401 gcacccggcg catggcgagg ccggtcagcg ggtcctcacg cgcctcgcgg tcgaggaggc
     2461 agcgctgcat ggagtgggac atctgcggca ggaggtggtc cagcaactcg atcgactcgg
     2521 cgttgagccg ccgcgggtcg caccagagct gcagccgggc gaggaccttg ccatcgaccc
     2581 gcaggtggtg gtcgatgatc tgccaggcgg cccggcggtg gacccccggc agggcgggcg
     2641 gatggcggtc cggctccggc acgccttcct ggaggacccc ctgcggcccc gcccaccagc
     2701 tcttgaactc gctgcccggc gccagcgcct cgaactggaa ccagaggaag ggcaccacct
     2761 tggcgcactc gacccggatg cgctccacca ccgcggccat ctcctgggcg gcgaagaaca
     2821 tccgcttgcc ggcgcggcgc agccgctcga ggtcgcgcac ccggcgctgc gcccgctcga
     2881 ggagcatcca gttgcgcacc gcctcgagga cgagcagggc gaagacggcg agcaggatgc
     2941 cgccgagccg ccaccccgcg gcgcggccgg cgagcgccag ggcgcccccc acggcccagc
     3001 cggcggcgtc gatggcgaag ggggggagga tcagccgcag ccggagcggc agctcggggc
     3061 gccggatctt gcggtcggcg agctcgagcc cgatccagac cccgaggtag gccgccgccg
     3121 cgccggtcca ggcgacgggc aggccccggt ccgaccgcag ccagctccag accacccccg
     3181 cggcgaggat cgccagggcg gcgcggccgg tgctctccag cgcccgcggc aacccgcgcc
     3241 gctccaccgg ctggagggcg aacagccggc gcgccagacg cagcgacagc tcggcgatca
     3301 ggaaggtgcc ggcgccgagc cagaccgccg ggaagacccc gcccaggagg agcatcgggg
     3361 ggaggaccgc ggtgcccagc ccctgcgtga ccggcccgcc gggcgtcgcg ggcccgccgg
     3421 ccagcggccg gtggcaggcg cccaccgtgc cggcgaagac catcaccagc agcagggtgg
     3481 gcgagggcac tcctgtgccg tcgagggccg cgaagcggga cgccacggcg agcaccagga
     3541 gggcccccgc cgtgggcagc agcgacgccc agagttcccg aggcgatcga gtgcgcttca
     3601 tcgaattctt tttcctgccg gcgttgggcg catggtacgc gttccgccaa ctcgccgcaa
     3661 atgacggccg caaatcgggg agctagccgg cgccaggcag gtcgcgggcg gcgggcagca
     3721 gcccgaagcc gtcggcctcc aggacggcgg tgaggatcgc ttcggccacc gcgcgctcgg
     3781 ccagaacccc gacctggttg acgtgggccg gccgggcgcc gctcgccagg gtgaccacca
     3841 ggtcgccgtc gaacagggag agcgccggcg ccagcgtccg gtagaagccg ccgaacgcca
     3901 tctggcagac cttgagggcg gtggccttgg tcaggacggc gtcggtcatc accaccgcca
     3961 gcgtggtgtt gccctcccag gggtgggggg tgtccggcgg cagggcggcc agaacccgct
     4021 cggccccggc cagctccagg gagccgggcg cggcgcggca gcccgccagg agccggccgt
     4081 cgagccgccg cacgtcgccg aaggcgttga ccgccaccgc cgccgccacg gtcacgccgc
     4141 cgggcaccgt caggctggcg aagccgaagc cccccttcat cccctgtgcc ggccccagcg
     4201 ccttgcccac cgtggcgccg gtccccgcgc cgacgctccc caccggcacc tcgccgctgg
     4261 ccgccgacgc cgccgactcc agggcggcct ccaccaaggc ggtccccggc cgcgccgtct
     4321 tgtcccccag cgccaggtcg aagaggatgg ccgtggggac ctgggggacc acggcgacgc
     4381 cggtctggaa gccgtggccg cgccgcgcca gccagtcgac caccggatcg gccgccgcga
     4441 ggccgaaggc gctgccgccg gcgagcacca cggcgtgggc gcggctcccc acgtggatgc
     4501 cggagaccag cgagtcgaac tgccgggtgc cggtggccga gccccgcacc tcggccgccg
     4561 ccatcatccc ttgggggcag aggacgacgg tgacgccggt catgccggcc cggtccgagc
     4621 cgtggccgat ggcgaagccg gggatctcca gccgacgcga ggggaggggg aaggggggcg
     4681 ggctcatggt ggagaatcct cgcacggctt gcgcgttagg cgatttgggt ctaggctatc
     4741 ccgctctgta gagctaaccc aggcaagagt gtatgcagga tcgaagcagc gacccctcgg
     4801 ccgtggtctg gaactggcgc aagccggggt ccgacgacgg cgacgcgcgg agccgggagg
     4861 cggccgcggc gcgcaagcgc ggcctcctcg gcctggccgt cggcttggcg gtggcggcgc
     4921 tgatctattt cttctggaag ccggtcctcg ccgccgtcgt cgccggcatc gccctggtca
     4981 tgggcctgat cgcgctcgcc gcccccctca ccctctacaa gaaggtgacg cgggcgctgg
     5041 acctgttcgc ccacggcgtg ggcatgacgg tcacctgggt gctgatgacc ctgctctact
     5101 atctgctctt cctgcccgtg ggggcggtgc tccgcgcccg ccgcaggctc ggcatcaccc
     5161 gttccttcga tccggccctg tccacctact ggaagacgac cggggagcgc gctcccgagt
     5221 cgtaccgcaa gcagttctga cgtgaagctc ctcggcatca gctgttacta ccacgactcg
     5281 gccgcctgcc tcatcgacga gggcaggatc gtggcggccg cgcaggagga gcgcttcacc
     5341 cgcaagaagc acgacgccgg cttcccctac aacgccgtgc gctactgcct ggcggccggt
     5401 gggatcggag aggggggcgg cgagctcggc ggggtggcct tctacgacaa gccgctcctc
     5461 aagttccacc gcctcctcga gacctacctg gccgtggtcc ccaaggggct gcgctcgtac
     5521 gtcatggccg tccccctctg gctgcgggag aagctctgga tcccgccgca catcctggag
     5581 gcgctggaga gctgcggtgt aggaggggac ggccgctcgc ccgacatctt cttcaccgag
     5641 caccacgagt cgcacgcggc gagcgcctac tacccctccc ccttcgcgga ggcggcggtg
     5701 ctcaccctgg acggcgtcgg cgagtgggcc accaccacca tcggcgtcgg cgaggggcac
     5761 cggctgcggc tgatccgcca gatcgacttc ccccactccc tggggctcct ctactcggcc
     5821 ttcacccact tcaccggctt caaggtcaac tccggcgagt acaagctgat gggcctcgcc
     5881 ccctacggcg aggggaagta caccgacctg atcaagcggg agctgatcga cgtccgcgag
     5941 gacggctcct tccgcctgaa catggactac ttcggctacc tcgacggcct ggagatgacc
     6001 aacgagcggt tcgcggccct cttcggcggg ccgccgcgca aacccgagtc gccgatcggc
     6061 aagcgcgaga tggacctcgc ccggtcgatc caggaagtga cggaggagat cgtcctccaa
     6121 ctcgcccgcc aggcgcggga gctcaccggc aagcgccacc tctgcctggc cggcggcgtg
     6181 gcgctcaact gcgtggccaa cggcatcctg ctgcgcgccg gcatcttcga cgacctgtgg
     6241 atccagcccg cggcaggcga cgccggcggc gccctcggcg ccccgctggc ggtctggcac
     6301 aacgccatga acaagccgcg ggtgaccgtc tccggcggcc gggacgccat gcgcggcgcc
     6361 ttcctggggc cggagttccc ggggccgcgc atcaaggagt tcctcgacga gcacggctac
     6421 gtctacgagg agctggccga cgccgactgg gcgccccgca tcgccgccct gctcgccggc
     6481 gagaacgtcg tcggtctctg ccacgggcgg atggagttcg ggccgcgggc gctgggcaac
     6541 cgctcgatca tcggcgaccc gcgctcgccg cgcatgcagt cgatcatgaa cctcaagatc
     6601 aagtaccggg agagcttccg gccgttcgcc cccagcgtgc tggtggagcg ggtgtccgac
     6661 tacttcgagc tcgaccggcc gtcgccgtac atgctgctgg tcgccgacgt ggtcgagggg
     6721 cggcgcatcc cgaagcgcgg ggacgaggcc ggcctgccgc tggtcgaatg ggtcaaccgc
     6781 ccgcgctccg acgtgccggc gatcacccac gtcgactact cggcgcgcat ccagaccgtc
     6841 gaccgggagg acaacccgcg ctaccacgcg ctgatctccg agttcgagcg gctgaccggc
     6901 tacggcgtga tcgtcaacac cagcttcaac gtgcgcggcg agcccatcat ctgcaccccc
     6961 gaggacgcct accgctgctt catgcggacg gagatggact acctggtgct cggaccgttc
     7021 ctgctggaca agaaggccca gcccgtcttc gaagagggcc gggactggcg ccaggactac
     7081 cagctcgact aggctaggca cggattctag gagaagcttt gatgcgcagg ctcggataca
     7141 tcggctccct gctcggcgag atcgtccagt tcgcccgcca gaacaaggcg tactggatcg
     7201 tcccgttcgt gctcatcctg gccctggtgg tcctgctggt ggtgaccagc caggcgacgg
     7261 cgccgttcat ctacaccctg ttctagttgc ccttccccga ggatgggtcc agaatagccg
     7321 cgatgcgccg tttcaagccg gtccatggat tgttcctggt ggtcctgttc gtcggggccg
     7381 tcctggccgc cgactacgcc ctcgaggggc ggttcggcgg cgggggcttc cggcgtgtgg
     7441 gcccggacaa gaacggcgtg gtgcggatcg acgtctccga cctccagccc cggcaggtcc
     7501 ggttctaccg cttcctgaac tacggcaacc aggaggtcaa gttcctcgtc ggccgggacg
     7561 ccgagggcca ggtgcaggtg ggattcgacg ccagcgagac ccacggcgcc gccggccgcg
     7621 gcttcaagca cgacggcgac tggctggtgg acaagaagtg cgagaccacc tcgcgcctgg
     7681 cggaggtcaa ctcgggccgg ggtggatgca ggccggtgcc tctgaagcac cagatcgtcg
     7741 gcgacgagct ggtgctggcg gagaacgaca tcctcttggg ctggcggtac ttccgctgat
     7801 ctcgcagccg ttcggcaacc aggggccaac ttgcgtggaa tcagagcttt gacggccccc
     7861 cctccagtgt tgtatcttcc cgccgtccgg aaaggatagc catgtcgtca gccgacttcg
     7921 tactctacga agaggagttc aagcatctgg aagaggcctt gcgccgtctg cggcaggagg
     7981 ccaacgcccg ggctgtcttc ctgatcgaca agaacggcca gcagatcgcc tcggccggag
     8041 agatcgagca gttcgacacc acctcgctgg cctcgctcac cgcgggcaac gtggcggcca
     8101 ccgacggcct cgccaagctc atcggcgagc gcgagttctc ggtgctgttc cacgaggggc
     8161 agcaggacca catccacatc tccatcgtcg ccaagcgggc gatcctggtg gtcatcttcg
     8221 atgaccgctc ctctctgggt ctggtgcggc tgcgggtcaa gcgcgccagc gtggacctgg
     8281 aaaagatctt cgacgtgatg gtgcagaagg cggagcgggg ggcccagggg cccgcccacg
     8341 ccagcccctt ctccgagatc acggacgacg acatcgacgc gctgttcggc gagtaggccg
     8401 gccggaagca gcggagcagc ggagagcgca atcgcaggtt cggttcccgg agagcctcga
     8461 cgcggtgcaa tagtctgcac cgcccgggca cgggtgacgg agaggcaccc aacatgactt
     8521 tcatcaacta cgccagcaag gagatcaact gcaagatcgt ctactacggg cccggcctgt
     8581 gtggaaagac gacgaacctg cagtacatct ataacaagac cgcgcccgag tcgaagggca
     8641 agatgatctc cctcgcgacc gaggcggacc gcaccctctt cttcgacttc ctccccctcg
     8701 acctggggag catccgcggc ttcaccaccc gcttccacct ctacaccgtc cccggccagg
     8761 tgttctatga cgccagccgc aagctgatct tgaagggtgt ggacggcgtc gtcttcgtgg
     8821 ccgacagcca ggaagagcgg atggaggcca acatcgagtc gatccgcaac ctcgaggcga
     8881 acctcaagga gcacggcttc gatctgtcga ccatccccta cgcgctgcag ttcaacaagc
     8941 gcgacctgcc gaacgtggtg gcggtcgagg agatgtaccg gaccctcaac tacaagcggg
     9001 agcccacctt cgaggccgtg gcgaccaccg gggtcggcgt cttcgacacc ctgaagtcgg
     9061 tggccaagca gatcctgatc gagctgagaa aacgctaagg gccggcgcca cgccgcaagg
     9121 agactgagcc gagatgaaca cgcccaatgc ggcgccggcc tccgccgacg cactcgacat
     9181 ctataccgca gggatcgccg ccctcggccg ccaggactgg gccgaggcgg tccgcctgtt
     9241 cgagaccgtg atccaggaga gcgacacccc cgagctcacc gggcgcgccc gccagttcct
     9301 ggccaccagc cgccagaagc tggcgcggaa tcagcaagag gatcggccca cgggagacga
     9361 cgacccctac ctccgggctc tcttcgagaa gaaccgggga gacttccggc aggccctgga
     9421 gctctgccgg caggacggcc gggaacagaa ggacgaccgc ttcgcctatc tcgccgcctc
     9481 gatccacgcg ctcgaggggc ggccggacga cgcggtccag acgttgaccc gggcggtcga
     9541 gctgaacccc aagaaccgca tccatgcctt tcacgacgcc gacttcgccg acctccgccg
     9601 caaccgggac ctccgccccc tcttcgggct ttcctgaggc ggcgcgcccc ggcagcgagc
     9661 tggtggccgt cggcggcggg accggcctct cggtcctgct gcgcggcctc aagcgtcacg
     9721 tcggcaccga gctcctgcgg cagctcaccg gcgtcgtcac cgtcaccgac gacggcggct
     9781 cgtccggccg cctccgccgc gagttcggtg ttctccctcc gggcgacatc cgtaactgca
     9841 tcgtcgcgct tgcggacgac tcggacgtcc tctcccggct cttccagtac cggttcccca
     9901 acggcgacgg cctgctcggc cactcgttcg gcaacctc
//
LOCUS       DASPJI010000120         9012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_297531, whole genome shotgun sequence.
ACCESSION   DASPJI010000120 DASPJI010000000
VERSION     DASPJI010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297531"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3258)
                     /locus_tag="VEL74_03360"
     CDS             complement(<1..3258)
                     /locus_tag="VEL74_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYG61594.1"
                     /translation="MSNGETNRALPTVEGIAIVGMAGRFPGSADVDALWRNLRDGIEG
                     ISFFSPEEMRAEGVSRETLALPNFVPAGGVLDDIDLFDAELFGFQPREVEVTDPQHRL
                     FLECAFEALERAGYDPFTEPGAIGLFAGCGLSGYLSEVCANPRAMAAVGSYRAVLGND
                     KDFLSTWVSYKLNLRGPSLSVQTACSTSLVAVHLACQSLLNGECDMALAGGASVPARQ
                     RSGYLHQAGGIHSPDGHCRAFDARAQGTVRGSGVAVVVLRRLSDALASGDRIHAVIRG
                     SAVNNDGALKIGYTAPSQEGQARVISEALSLAGLSPDDISYVEAHGTGTALGDPIEIA
                     ALTQVFRAGTRRRGFCGIGSVKTNLGHLDTASGIAGLIKTALALEHRQVPPSLHFETP
                     HPNIDLKDSPFYINAWLTPWEPSTPGGLRRAGVSSFGIGGTNAHAVLEEAPALPPADP
                     SRRLQHLFVLSARNEAALAAATSRLADHLEANPDLAPSDVAYTLQVGRKAFEHRRALV
                     CSNLAEAVATMREGNPEKVWTRHERARFRPVAFLFPGQGAQHPGMARELYDGEPTFRD
                     LVDHCAERLAPELGLDVRHVLYPPDGDDAAGERLEGTGLAQPALFVVEYALARLWMEW
                     GVEPQAMLGHSVGEYVAACLAGVFSLDDALGLVAARGRLMQELPAGAMLAVALPERDV
                     QSLLGESVAVAAVNGEARCVLSGTVDAIERLDRHFVDLGIECRRLHTSHAFHSPMMDP
                     ILEMFLARVREVRLRPPRIPYISNLSGTWITAAEATAPGYWVRHLRETVRFSAGAAEL
                     LAEPDRILLELGPGLGLTNLVKQHPARRAEQPVIAALPHPRQRESDLKTSLKALAQIW
                     TASGRVDWRGFHLYERRRRVLLPTYPFQRKRYWVESEAPPAPVTSPTVAASVAPVFQA
                     LPAGSVLSSPEPAQRAEVRMSPIVETSVPARSRRDDILSILCEIVRELTGSAPGAAQI
                     HTHFLEMGIESLLMIRATQALEDKLGVRISVVQLFEELTTLDLLAGHLEQMVPPDRQL
                     AHLLPAGTSAPAAAAPAAAAPAAAAPAPAVSPAAPVLQVAPAMVSQSYVEHAPALA"
     gene            complement(3255..6476)
                     /locus_tag="VEL74_03365"
     CDS             complement(3255..6476)
                     /locus_tag="VEL74_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61595.1"
                     /translation="MKPEEIDDIYELSPLQQGMLFHSLAAAGSGVYVLQFVCPLTGEL
                     DLDAFRRAWERMTARHSVLRTSFHWQELDKPLQVVQHAVELPLAQLDWQSLTAAEQEP
                     RLAALLHDDRQQGFALTQGPLVRLTLIRLDKDLCNLILTVHHIVLDGWCLGLLLRELL
                     ELYEGFRQGREVRLPPVRPYAEYIDWLQSRDAAETEAYWRGMLAGFTTPTPLSVDHPA
                     AEGEEGVLFGEEGLLVPAALVSDLQSFAARRQLTLSTLVQGAWVRLLAEYSGEEDVLY
                     GSVVAGRPPDFPGIESMIGLFVNTLPVRARLSGSQETAEWLRDFQRLQLDLRRFEHSS
                     LIDVQGWSEVPRGRPLFESLFAFENYPRKSSVAEVQESRGALRLGNVRTYEQTNYPMT
                     VAVGPGRELSLRASFDRARFEAPAVKRMLHHLRALLAGLVGVAERPLAEWSCLTAPEH
                     HQLLREWNDVAATVPPWSPLELLAERTRLQPDAPALVDCDHYLTYGELSRRATALARH
                     LATLGVGPEIRVALCVPRSVELVVAMAGVLEAGGAFVPLDPAYPSDRLGFLVGDSAAA
                     VLLTVPELLPALSGLTARVVCLDPSLLPADGDEGFSREADLDGLAYVIYTSGSTGLPK
                     GVAVRHGGLTNLVLWHERTHGITPSDHLSQVASPAFDAAVWEIWASLASGATLHLADE
                     ATRLSSARLVEWVVERGITSGFLPTPLAEAALAEDWPAVTPLRSLLTGGDRLRCGAPG
                     AGFRLLNHYGPTESTVVGTWCPVPRQEDLAGVPPIGRPISNLRAYLLDRRGWPVPVGV
                     AGEVCLTGAGLARGYLGRPELTAASFVPDPWAGQPGARLYRTGDLGRFLPDGRIEFLG
                     RVDRQVKVRGFRIEPGEIEAMLARHASVREAVVVQGEDGALVGYAVKRPEADVSEPVL
                     RAFLREHLPEHMVPQALLLLDSLPLTSNGKVDWRALVSPVAGGGQALELTAPRNDLER
                     TIAAAWQEVLGLQMVGVEQSFFDLGGHSMLVLQVQKRLEQALKRPLAVTDLFRYSTIA
                     RLAEHLAGGEADESVDTGQQRAEARRASLQRRRRSGAVKEIEL"
     gene            complement(6513..>9012)
                     /locus_tag="VEL74_03370"
     CDS             complement(6513..>9012)
                     /locus_tag="VEL74_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61596.1"
                     /translation="LTLSTLVQGAWARLLGQYSGEEDVLFGSVISGRPPDFPGVESMV
                     GLFVNTLPVRTRLRPEPAVSSWLREFQMLQLELHRYGHSSLLDVQGWSEVPRSRPLFE
                     SLVAFENYPRRAHTAGRQEGTAALEMGGISSFEQTNYPLTLFAVPGAGIALRASYDPQ
                     RFEAMAVRRMLVHLRSFLEALNEVAEAPLAGWSCIGAAERQEILGWSAGPPTAAPASS
                     IGRLLSNQAARTPDAVAVTGDGAQVSYQELNRRARRLARRLRSLGVGPEQRVAICTKR
                     SLDLVTGILGVLEAGGAYVPLDPAYPRERLSFMLEDSGAAVLLTRHELLGSLPPTTAR
                     VVCLDEEHLALPSQDTLPSWGEAEPDHLAYVIYTSGSTGRPKGVAIEHRSVLALLDWA
                     RSSFAPEELAGVVAATSVCFDLSVFELLVPLSLGGRVILVENALHVASDEAATGATLL
                     NSVPSAVTGLLRGGLPPGVQTVNLAGEPLKRSLVDELYRQAGVRRVLNLYGPSEDTTY
                     STIATLEPGSADAPDIGRPVSGTRIYLLDGSLFPVPTGLAGELCIAGAGLARGYLGRP
                     ELTAERFVPDPFGSTGERLYRTGDRARYLLDGRLDFLGRVDLQVKVRGFRIELGEIEA
                     ILASHPGVLEAVVVAHHDAAGEKRLVAFLRLSAEQEPTGTELRDLLRGHLPEPMVPST
                     FVALDDMPLLPNGKVDRRALAARDLEPAALPSSFAAPRNELERQIAAIWCEVLGLPQV
                     SVQDSFFDIGGHSLLVLQVQKRLQETLETPLAITDLFRFPTIALLAEKLSDQGSADSS
                     APATGRERAEARRASMQRRQSALAVEEDDAQSST"
BASE COUNT         1565 a         3134 c         3056 g         1257 t
ORIGIN      
        1 ggccagagcc ggggcgtgct cgacgtagct ctgcgagacc attgccggag ccacctggag
       61 caccggggcg gccggagaga cggccggcgc cggagccgcc gcagccggag ccgccgcagc
      121 cggagcggcc gcggctggag ccgaggtccc ggccgggagc aggtgtgcca gctgacggtc
      181 cggcggcacc atctgttcga ggtggccggc caacaggtcc agagtagtga gctcctcgaa
      241 gagctgcacc accgagatgc ggacgcccaa cttatcttcc agcgcctgag tggcccggat
      301 catcagcaac gactcgatcc ccatctcgag gaaatgggta tggatctggg ccgcgccggg
      361 cgccgagccg gtcagctccc ggacgatctc gcacaggatc gacagaatgt catcgcgccg
      421 ggacctcgcc ggcacgctgg tttctacgat gggggacatc cgcacctccg ctctctgcgc
      481 cggctcggga ctggacaata ccgatcccgc gggcagggct tgaaaaaccg gggccaccga
      541 ggcagccacc gtaggcgacg ttaccggcgc cggaggagcc tccgactcca cccagtaccg
      601 tttacgctgg aagggataag tcggcagcaa cacgcgccgc cgccgctcgt aaaggtgaaa
      661 gccgcgccag tcgacgcgac cgctggcggt ccagatctga gccaacgcct tgagcgaggt
      721 cttcaggtcc gactcgcgct gccgcggatg cggcagcgcc gcgatcaccg gttgctccgc
      781 ccgcctagcc ggatgctgtt tcaccaggtt ggtcagaccc aggccgggcc ccagctccag
      841 gaggatgcgg tccggctctg ccagcagctc cgcggccccg gcggagaaac gcaccgtctc
      901 ccgcaggtgg cgcacccagt agccgggagc cgtggcttcg gcggcggtga tccaggtgcc
      961 ggagagattc gagatatagg ggatgcgggg cggccgcagc cggacctcac gaacacgcgc
     1021 caggaacatc tccaggatcg ggtccatcat cggggagtgg aaagcgtggg aggtgtgcaa
     1081 cctccggcac tcgatgccca ggtcgacgaa atgccgatcc agacgctcga tggcatctac
     1141 ggtgccggag aggacgcaac gcgcctcccc attcaccgca gccaccgcga cgctttcccc
     1201 gagcagagat tgcacgtccc gctccggcag ggcgacagcc agcatggcgc ccgccggcag
     1261 ctcctgcatg aggcggccgc gcgccgctac caatcccagt gcgtcgtcca gggagaacac
     1321 gccggcgagg caggcggcca cgtactcgcc cacagagtgg ccgagcatgg cctgcggctc
     1381 gacaccccac tccatccaca agcgtgccag cgcgtactcg acgacgaaaa gggccggctg
     1441 ggccagcccg gtcccctcca gccgctcccc ggcagcgtca tccccgtccg gtggatagag
     1501 gacatgccgg acgtcgagcc ccagctccgg cgccagacgc tcggcacaat ggtcgaccag
     1561 atcgcggaag gtgggctcgc cgtcatagag ctcgcgtgcc atgcccgggt gctgcgcgcc
     1621 ctggccgggg aacaggaacg ccaccggccg gaagcgcgct ctctcgtgac gggtccagac
     1681 cttctcggga ttcccctccc gcattgtagc gaccgcctcg gcgaggttgg agcagaccag
     1741 cgcgcgccgg tgctcaaagg ccttgcgtcc cacctggagc gtgtatgcga catccgaagg
     1801 cgcgaggtcc gggttagcct ccaggtgatc cgccagccgt gacgtcgccg ccgcgagagc
     1861 ggcctcgttg cgggcggaga ggacgaagag atgctgcagc cgccgggagg gatcggcggg
     1921 cggcagggcc ggcgcctcct ctagcacagc gtgagcgttg gtgccgccga tgccgaagga
     1981 gctgacgccg gcgcgccgca gcccacccgg agtcgaaggc tcccaaggcg tgagccaggc
     2041 gttgatatag aaagggctgt ccttcagatc gatgttggga tgcggcgtct cgaagtgcag
     2101 gcttggcggc acctgacggt gctccagagc cagagccgtc ttgatcaggc cggcgatccc
     2161 ggacgccgtg tccaggtggc cgaggttggt cttgaccgag ccgataccgc agaatccgcg
     2221 gcgccgggta ccggcccgga atacctgagt cagggccgcg atctcgatgg gatcgcccag
     2281 agcggttccc gtcccgtggg cttccacgta gctgatgtcg tcaggcgaaa gcccagcgag
     2341 cgacagcgcc tcggagatga cccgcgcctg cccttcctgg ctgggggccg tgtagccgat
     2401 cttgagcgca ccgtcgttgt tgacggcgga cccgcggatc accgcgtgaa tccggtcgcc
     2461 gctggcgagc gcgtcggaga ggcgccgcag gacgacaacc gccactccgc tgccgcgcac
     2521 cgttccctga gctcgggcgt cgaaagcacg gcagtggccg tccggggaat ggattccacc
     2581 ggcttgatgg aggtagccgg accgctggcg ggcaggcacc gaggcaccgc cggccagcgc
     2641 catgtcacac tcgccattga gcaggctctg gcacgccagg tggaccgcca ccagcgaggt
     2701 cgagcaggcc gtctggacgc tcaggctggg tcccctcaga ttcagcttgt aggaaaccca
     2761 ggtggagagg aaatccttgt cattgccgag gaccgcacgg tagctgccca ccgccgccat
     2821 ggcccgcggg ttggcacaga cctcggacag gtagccgctg aggccgcaac cggcgaacag
     2881 accgatggcg ccgggctcgg tgaagggatc gtagccagcg cgttccagcg cttcgaaggc
     2941 acactccagg aagagccggt gctgaggatc ggtgacctcc acctcgcgtg gctggaagcc
     3001 gaagagctcg gcgtcgaaga gatcgatgtc gtcgagcacc ccaccggcgg gcacaaagtt
     3061 cggcagcgcc agggtctcgc ggctcacccc ttcagccctc atctcctccg gagagaaaaa
     3121 ggagattccc tctataccgt cgcgcaggtt gcgccagaga gcgtcgacat cggccgaacc
     3181 ggggaagcgg cctgccatac cgacgatggc aatcccttcg acggtcggga gcgcgcggtt
     3241 cgtctcgccg ttgctcatag ctcgatctcc ttcacggctc cggaacgtct gcggcgctgg
     3301 agcgaagccc ggcgggcttc ggccctttgc tggccggtgt ccaccgactc gtcggcttcg
     3361 ccgcctgcca gatgctcggc caagcgagcg atcgtggaat agcggaacag atcggtgacg
     3421 gccaggggcc gcttcagcgc ctgctccaat cgcttctgaa cctggagaac cagcatggag
     3481 tggccgccca ggtcgaagaa gctctgctcc acccccacca tctgcagccc cagtacctcc
     3541 tgccaggccg ctgcgatagt gcgctccagg tcgttgcgcg gcgccgtcag ctccaacgcc
     3601 tgcccgccgc ccgccaccgg agagacgagg gcccgccagt ccaccttgcc gttggacgtg
     3661 agcggcaggc tatcgaggag gagcagcgcc tgcgggacca tatgctcggg caggtgctcc
     3721 cgtaggaagg cgcgcaggac cggctcggag acgtcggctt ccggccgttt taccgcatag
     3781 cccaccagcg ctccgtcctc cccttggacc accaccgcct cccgcaccga ggcgtggcgc
     3841 gccagcatcg cctcgatctc tcccggctcg atccggaagc cgcgcacctt cacctgccga
     3901 tccacacgtc ccagaaactc gatccgtcca tcgggcagaa agcgccccag gtcgccggtc
     3961 cggtaaagcc gtgcgcccgg ctgtccggcc cacggatcgg ggacgaacga cgcggcggtc
     4021 agctcgggcc ggcccaggta gccacgcgcg aggcccgcgc ccgtcaggca gacctcgcca
     4081 gccacgccga ccggcaccgg ccagccccgg cggtcgagca gataggctct gaggttggaa
     4141 atgggacgcc cgatgggcgg caccccagcc agatcctcct gacgcggcac cgggcaccag
     4201 gttcccacca ccgtggattc ggttgggccg tagtggttga gcaggcggaa gccggcaccg
     4261 ggcgccccac accgtaaccg atcgccgccg gtgagcagag agcgcagggg tgtgaccgcg
     4321 ggccagtctt cggcgagagc cgcctcggcc agaggtgtcg gcaggaaccc cgaggtgatg
     4381 ccgcgctcca cgacccattc caccagccgc gccgacgaca gccgcgttgc ctcgtcggcc
     4441 agatgcaggg tcgccccgga agccagggag gcccagatct cccacaccgc tgcgtcaaag
     4501 gccggcgagg cgacctggct caggtggtcg gagggggtga tgccgtgcgt cctctcgtgc
     4561 cacaggacga gattcgtcaa cccgccgtgg cggaccgcca ctcccttagg gaggccagtg
     4621 gagccggagg tgtaaatcac ataggccagg ccgtcgaggt cagcctctcg cgagaagccc
     4681 tcgtcgccgt ccgcgggcag caggctaggg tctaggcaga cgaccctcgc ggtaagaccg
     4741 gagagagccg ggagcaactc cggcacggtg agcaggaccg ccgctgccga atcccccacc
     4801 aggaagccaa gccggtcgga ggggtaggcc ggatcgaggg ggacaaatgc gcctccagcc
     4861 tcgagaacgc cggccatggc gaccaccagc tccaccgaac gcggcacgca gagggccacg
     4921 cggatctccg gtcccactcc gagagtagct aggtggcggg cgagggcggt ggcgcgccgg
     4981 ctcagctcgc cataggtgag gtaatggtcg caatcgacca acgccggggc gtccggctgc
     5041 agccgggtcc gctccgccag cagctccagc ggcgaccagg gagggacggt cgccgcgacg
     5101 tcgttccatt cgcggaggag ttggtggtgc tccggcgcgg tgaggcacga ccactccgcc
     5161 agaggccgct cggctacgcc taccagcccg gccagcagag cccgcagatg gtgcagcatg
     5221 cgcttcacgg caggggcctc gaagcgagcc cggtcgaagc tcgcccgcag cgacagctcg
     5281 cggcccgggc cgacagccac ggtcatggga tagttggtct gctcgtaggt gcggacgttg
     5341 cccagccgca atgcgccgcg gctctcctgc acctccgcca cactggactt tcgcggatag
     5401 ttctcgaagg cgaaaaggct ctcaaacagg gggcgcccgc gcggcacctc gctccagcct
     5461 tggacatcga tcagggagct gtgctcgaag cggcgcagat cgagttgcag ccgctggaaa
     5521 tcgcggagcc actccgcggt ctcttgtgac ccgctcaggc gcgcccggac cggcagcgtg
     5581 ttgacgaaga ggccgatcat cgactcgatg ccgggaaaat ccggaggacg tccggcgacc
     5641 accgacccgt agaggacgtc ctcctccccg ctgtactctg cgaggagcct tacccaggcc
     5701 ccttgcacca gggtgctcag agtgagctgc cgcctggcag caaagctctg gagatcgctc
     5761 accaacgccg ccggtaccag caggccctcc tcgccgaaga gcacgccttc ctctccctca
     5821 gcggccggat ggtctacgct cagcggcgtc ggcgtggtga aacccgccag catgccgcgc
     5881 cagtacgcct cggtctccgc ggcgtcccgg ctctgcagcc agtcgatgta ctcggcatac
     5941 ggacgtaccg gcggcaggcg cacctcgcgt ccctggcgga agccctcgta gagctcgaga
     6001 agctcccgaa ggagcagccc cagacaccac ccgtccagca cgatgtggtg gaccgtcagg
     6061 atcaggttgc agagatcctt atccagccgt atgagagtca accgcacgag cggtccctgg
     6121 gtcagagcga agccctgctg gcgatcgtcg tgtagcagcg ccgccagccg cggctcctgc
     6181 tcggccgcag tgaggctttg ccagtcgagc tgcgccagcg gcaactcgac cgcgtgctgc
     6241 accacctgca gcggcttgtc cagctcctgc cagtggaagg aggtgcgcag cacggaatgg
     6301 cgggccgtca tccgctccca ggcgcgccgg aaagcgtcga gatcgagctc gcccgtcagc
     6361 ggacagacga actgcagcac gtacaccccc gacccggctg cggccaggct gtggaagagc
     6421 atgccctgtt gcaggggcga gagctcgtag atgtcgtcga tctcttcagg cttcatgggt
     6481 cgtcttcttc cttccgcggc accagtcctc ccctaggtcg aggactgagc gtcgtcctcc
     6541 tccaccgcca aagcgctctg gcgccgctgc atggacgccc ggcgggcctc ggccctctcc
     6601 cttcccgtcg ccggagccga cgagtcggcg cttccctgat cggagagctt ctcggccaga
     6661 agcgcgattg tggggaagcg gaacaggtcg gtgatggcca gcggcgtttc cagagtctcc
     6721 tgtaatcgct tctgtacctg cagaaccagc aaggagtgcc ccccgatgtc gaagaagctg
     6781 tcctggacgc tcacctgcgg caggcccagg acctcgcacc agatggcggc gatctggcgc
     6841 tcgagctcat tgcggggagc ggcgaaggac gagggcaggg ccgcaggctc gaggtcgcgg
     6901 gctgcaaggg cccgccggtc taccttgccg ttgggcagga gcggcatgtc atccagagcc
     6961 acgaacgtgg acggcaccat aggctccggc aaatggccac gcagcagatc gcgcagctcg
     7021 gttcccgtcg gctcctgctc ggccgacagg cggaggaacg ccacgagccg cttctccccc
     7081 gctgcgtcgt gatgcgccac cacgaccgcc tcgagcacgc ccggatgaga cgccaggatc
     7141 gcctcgatct caccgagctc gatgcggaag cctcgcacct tgacctgcag atcgacacgt
     7201 ccgaggaagt caaggcggcc atctaaaagg tagcgcgccc ggtcaccggt ccggtagagc
     7261 cgttctcccg tcgagccaaa gggatcgggc acgaaacgct ccgccgttag ctccggccgt
     7321 cccaggtaac cgcgtgccag gccggcaccg gcgatgcata gctcccccgc cagacccgtc
     7381 ggcaccggaa agagggagcc gtcgaggaga tagatgcgcg ttcccgagac cggccggccg
     7441 atatcgggag cgtcggcaga gcccggctcc agagtcgcga tggtcgagta ggtggtgtcc
     7501 tcggaaggac cgtagaggtt gagcacccgg cgcaccccgg cctgacggta cagctcgtcc
     7561 accaagctac gcttgagcgg ctcccccgcc aggttgacgg tctgcacgcc ggggggcagc
     7621 ccaccgcgca gcagcccggt gaccgccgag ggcacggagt tgaggagagt cgccccggtg
     7681 gcggcctcgt cggaggccac atggagcgcg ttttccacca ggattacccg cccgccgagg
     7741 ctcagcggca cgagcaactc gaagacggag aggtcgaagc agaccgaggt ggcggcgacg
     7801 acgcccgcca gctcctcggg agcgaagctg ctgcgagccc aatcgagcag cgccagcacg
     7861 ctgcgatgct cgatggccac tcccttgggc cggccggtcg agcccgaggt atagatgacg
     7921 tacgcgagat ggtccggttc ggcctctccc caactcggaa gggtgtcctg cgacgggagg
     7981 gccaggtgct cttcgtcgag acagacgacc cgggcagtgg tcgggggcag gcttccgagc
     8041 agctcatgcc gggtgagcag caccgccgcc ccggagtctt cgagcatgaa agacagacgc
     8101 tcccgcgggt aggcagggtc gagcggcaca taggcaccgc ccgcctcgag cacacctagg
     8161 atgccggtaa ccagatcgag cgaccgcttg gtgcagatag ccactctctg ctcgggtccg
     8221 acgcccaggg agcgcaggcg acgggcgagt cggcgggcac gccgattgag ctcctgatag
     8281 ctcacctgag cgccgtcacc ggtcaccgct accgcgtccg gcgtcctcgc cgcctggttc
     8341 gacagcaggc gtccgatgga cgacgcgggc gcggccgtcg gtggaccggc gctccagccg
     8401 aggatctcct gccgctcggc ggcgccgata caggaccatc cagccagggg ggcctccgcc
     8461 acctcattga gcgcctccag gaaagaccgc aggtggacga gcatccgccg caccgccatc
     8521 gcctcgaacc gttgcgggtc atagctggcc cgtaaagcaa tcccggcacc cgggacggcg
     8581 aaaagtgtca gcggataatt ggtctgctcg aaggagctga tgcctcccat ctcgagcgcc
     8641 gccgttccct cctgccgtcc ggccgtatgg gccctgcgcg ggtagttctc gaaggcgacg
     8701 aggctctcga agagaggcct gctgcgcggc acctcgctcc agccctgaac gtcgagcagc
     8761 gagctatggc cgtagcggtg gagctcgagc tgcaacatct gaaactcgcg cagccagctc
     8821 gacaccgccg gctcggggcg caggcgcgtc ctgaccggca gcgtgttgac gaacaaaccc
     8881 accatggact cgacgcccgg gaagtcggga ggccgccccg agatcaccga cccgaacagg
     8941 acgtcctcct cgccgctgta ctggccgagc agacgggccc aggctccctg gaccagggtg
     9001 ctcagggtaa gg
//
LOCUS       DASPJI010000121        17697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_469570, whole genome shotgun sequence.
ACCESSION   DASPJI010000121 DASPJI010000000
VERSION     DASPJI010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17697)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17697
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469570"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..1690
                     /locus_tag="VEL74_03375"
     CDS             290..1690
                     /locus_tag="VEL74_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61597.1"
                     /translation="MSSPVLTEELREAHRSLSEVDLRFREYASASPQLLSYAEFRVPE
                     SYREMLSFRLQPWPTFIDRHKREEIARVSVAVSDLVKSVPHRVFGGDPVAVSRFYGLA
                     SPEEAEDLLSEPNGIAEIVARGDLIATADGFKCIEFNFATRLGGWESTILSSLHLSSP
                     AIIRFLQQEGIRPAVTPTTRCFFRHILEEVERRGLARGVGVNIAFAFHERLARTPIII
                     QGVARLGAEWDQVCRETGRQGKGRLLAATWQELSPGSSGQGLSCRGLPVHAVVEQTTE
                     PVPEPVYRCFKEGGTCLFNTPVTTILTSKMNVALLSEHEGSGRFSPEEREVIRRHIPW
                     SRRVVRDTVDFRGETAYLPDLLAARKEDMVLKEAQNLGGKGVAVGRFTAADRWADLCR
                     SALENGEWIVQEYLESTSYLFQSGDHGCTPHNVIWGPFVFGSTYAGLILRMQSKAVPG
                     VVNLSQTATEGVVFEV"
     gene            1737..3095
                     /locus_tag="VEL74_03380"
     CDS             1737..3095
                     /locus_tag="VEL74_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYG61598.1"
                     /translation="MRFGIPTLALLAIPTLLLAQAPAPRPAAPGTPETPEVIALRAAR
                     LFDGKGDTAVRNGVVIIEGSTIKAVGSGIPVPAGARVIDLGDATLLPGLIDAHTHITG
                     ESSDNWLQDTVSGLRRDVPESAIRSTEFARRTLMAGFTTVRDVGSGEYVDVGLRNSIR
                     NGIVPGPRILAAVHSLGARGGHCDESGYPYQRFGHESGIEDGIASGPDQFRDSVRFQI
                     KYGADVIKVCATGGVLSLNDEVDTPQVTQEEMNAIVEEAHRLRKKAAAHAHGAEGAKV
                     AIRAGIDSIEHGSFLDDEALRMMKQRGTYLVPTLLAGEYVAGKAAVRQYPPEIAEKAR
                     AAVAARSATFRKALQIGVKIAFGTDSAVSPHGLNAQEFALLVDHGMSPAAALRSATSA
                     AADLLGLAKTIGTLEPGKEADIVAVPGDPLADIRAMEKVRFVMKGGRVFKDESQPGPA
                     GK"
     gene            complement(3071..3505)
                     /locus_tag="VEL74_03385"
     CDS             complement(3071..3505)
                     /locus_tag="VEL74_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HYG61599.1"
                     /translation="MTLDPEKLLNKRQFIRGAVQRLEEIRARGREAFLADPLLQDAAL
                     RNLQVAIEAMIDAANHIIAREGLGVPRRYRDSMDLLVEHGILPAEHKETFRRMTSFRN
                     RIVHLYEDVDPEEVADVLEHRLGDFEIFLTALAKRYFPAGPG"
     gene            complement(3495..3947)
                     /locus_tag="VEL74_03390"
     CDS             complement(3495..3947)
                     /locus_tag="VEL74_03390"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYG61600.1"
                     /translation="MRWREGQPPIDVEAYLPDLRAYLEGLDGLAAAWIHGSYGTPYQT
                     PLSDLDLALVFRPDKVPAFREELGIAADIQQILHEDDASVTVLNRSPVIFQFRVLETG
                     RQLVCNDPVALADFTERVLNEHGDFIVDHEQFLRDYDQAFTERYGRDA"
     gene            complement(3995..6493)
                     /locus_tag="VEL74_03395"
     CDS             complement(3995..6493)
                     /locus_tag="VEL74_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG61601.1"
                     /translation="MSRRWRWRDWPWAAKLSLLLGGLALVPLTAVTLFNEAVARAGLI
                     RDARAQNLQQARNTASILENLLDDLRADLSVAATAPQTVSFFTGWRRHALDREVEGIL
                     RQIRDAQKLDALYLMEPGGTVRLASEPRFRGRSYVSAPYFRDAAAGNTLVDEPRWDPA
                     DREVYLHAATPIRGEAGRIAGVAVARVRLARIDELIRADTDFAGRGEFGVLWNADGIR
                     LSHPTHPSLRFHPLEPLPADVAARLIGESRFGPATAALLARPRPAAGLVERSRWLLYD
                     RGRDPYLELEGPGGVPLHAAVVPLPRTRWLYGIFSPEPAILASVSQQTRRSLVFAAVT
                     GLLAIALALSAAGWATRPLRLVGQAANALARGDMSRRVRLDRDDELGRLAAAFDAMAD
                     ALADKEGELRGHATELERRVEEQTAALRRSEAELRVLFESEQEARRKAEEANRIKDEF
                     LSTVSHELRTPLNAILSWVWLLSNGRLTGDEAGRGLATIERNARAQGQIIDDLLDVSR
                     IITGKLLLQVVPLNLIQIIDAALDSVRPAADAKGIRIESRLDPLAALAAGDPNRLQQV
                     VWNLLSNAVKFTPKGGTVEVSLLAGDAYTAIRVRDTGIGISPHFMQHVFDRFRQADST
                     TTRTHGGLGLGLAIVRHLVELHGGTVEAESDGEGRGAVFTVFLPVAPELRLGAGPEAP
                     ERAAVPADGDGLDLQGLKILLVDDEDDTREVLPALLARFGAEVEAAASTPEALAALPR
                     FQPDVLVSDIAMPGEDGYALIRRVRALDGNLGDLPAIALTAQAGAGDRRQALEAGFQM
                     HLAKPVEPRDLVAAVLSVARRTLRGTPPGTPPPR"
     gene            complement(6490..8070)
                     /locus_tag="VEL74_03400"
     CDS             complement(6490..8070)
                     /locus_tag="VEL74_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYG61602.1"
                     /translation="MNNHLKSLTLAAALLLSFAGCGRETPEPRPARQPERKGGGGELR
                     VILPLEPRVLDPNSMRDEAALVVAPSVFSKLVTLDVDARLLPQLAESWTTSPDGRVYT
                     FRLRDGVRWHDGHPFSADDVRWSLTALGREPSFATEVFQRIAAIETPDPRTVVIRLRE
                     PWSPFLSMLAWYGTYILPRHRQEARGAHGFQPGELPVGTGPFRVESWEPGRSITLAAN
                     RAYFRPGPYLDRVVYLLRSAGEGVDLLLAGQADYFGVRPPVDRLPELARSPDLAILTR
                     PSDSRFYCGFNLRRGPLADRRVREAVNRAIDRKALLEKAFAGYGAPALGFYTPAVAWA
                     YDAQARVPDYDPERARALLDEAGLRPGPDGVRVRWDMVAPDILPIPDMVRVLAAQLRE
                     VGVEIRPVLLPLAEWAPRLLNNHDFDLGIMGGNQGPDPENLNLRFGSRGPTQFMGYSS
                     PELDAAVAEGSRAADLPRRAAAYFRAQRILARDLPIAPLAESVYIAVMRRQVHGTPHA
                     EARGLVPNNDFSLVRLEP"
     gene            8242..9153
                     /locus_tag="VEL74_03405"
     CDS             8242..9153
                     /locus_tag="VEL74_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61603.1"
                     /translation="MKMFLKSIAAFVFLALVIWAIRSDSLEERGNLGAAPLAGAAVPA
                     GDPAWRRAPLWDDGKSEFCAYEATWPRYGHRFEGRALLVLVKEPWAPDLNVKADNPRP
                     GGFEVLKLNHVRDVPTGIYSYHQMASVYSHRESGALVKLAATSAEACGISGAEMVGGR
                     LATRSYFDGQGDRSLPWPAEALPEDGLPAALRDYVTGPVPDHLSVFPSLMASRLPELA
                     PSIYKVERRAAGEVETPAGASPGVEIRLTRGKSWLSYTFEAEAPHRLLRLQSHDGTEY
                     RLAKCDRIAYWQMHNPGDESWLPESTR"
     gene            complement(9517..10752)
                     /gene="rocD"
                     /locus_tag="VEL74_03410"
     CDS             complement(9517..10752)
                     /gene="rocD"
                     /locus_tag="VEL74_03410"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HYG61604.1"
                     /translation="MSTTVLEDTSALTTADYIALEDRYGAHNYHPLDVVVSRAQGVWV
                     WDVEGRKYLDFLAAYSAVNQGHCHPGILQAMLEQASRVTLASRAFRSDQLGPFYKQIC
                     ELGGFDKFLPMNTGAEAVETAIKAARKWGYTVKGIPDGQAEILVFANNFHGRTSTIVG
                     FSTEEQYKSGFGPFAPGFRILPYGDADAVAQAMNPNVAAVLVEPIQGEAGIVMPPDGY
                     MKALREITREHNALLIADEIQSGLGRTGKLFCFQHEGIQPDMVIVGKALSGGFYPISG
                     ILANDPVMGVFKPGDHGSTFGGNPMAAAIGRAALRVLVEEGMVENSAKLGPYFIERLR
                     AIESKHVKEVRGKGLWIGIELHPEAGGARRFCEALKQDGLLCKETHDNVIRVAPPLTI
                     QQSEIDWAVERIADVLTRL"
     gene            10916..12574
                     /locus_tag="VEL74_03415"
     CDS             10916..12574
                     /locus_tag="VEL74_03415"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HYG61605.1"
                     /translation="MVDYSNIPRDSRRVPLETRVQFKFDRFFGFLSEYSSNISPGGMF
                     INTQQPQPPGTVLDVEFSLGEDGFQLIQARGEVIWVRKEDQGPGRPAGMGLRFLELSD
                     GSKELIYRMVDNYVQEGGVPFDLSLVPDEMPAAAKAAFPGAAAAAASLPRTPPANDDE
                     PFDLSADPFQLEDMEPAVQPRQEVLVFPDIDTPAFPEPEADPLPDLDSLAPPPGGWAP
                     QPAPEERPGEMPRELLEPLPEPAPWAAAEPQPEVPAEPSAPLLAREEPEPARWTSAPP
                     PLEPESQSWSGVPRDPEAAPPPVYLPEPPPPREAPTPLVHYAGSARATEPRRSSVPWL
                     IVLLVAVGALTAFVLMNEQIMDWAGLGSGEITAATPGRPLPRPRARATVPAPGGPPAA
                     TVTEGTPAAPVVIEEAPGATQPAAPAPSAPGAQTAAPAPVAAAPAPATGPPATVIQRI
                     TFERLPTGTEIVLWGNGAIRLGSYTRLRIDGNPAREVIKLKGIRQRYPSPRVGVGTPE
                     VLQVRVGHHPGDELHVVLDLTGPRVQAAGFQEDGNRLRVLVQRP"
     gene            12736..13815
                     /locus_tag="VEL74_03420"
     CDS             12736..13815
                     /locus_tag="VEL74_03420"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HYG61606.1"
                     /translation="MTSDVHRDVSRAKRQMLRELVRENVDLKQGRLPRRLRARPSVWH
                     RLPPAARIAIAVVLICGVFGSSWLLWALSAEPVNLTIMAGRPQGSPVAQPAVSTEPRP
                     GPAVDASVFPLAVRRVVIDPGHGGDSNGTRAPLGLVEKELALEISQRLRRLLERDGFQ
                     VLMTRQDDRAVTLDQRAAVANEAGADIFVSIHLNWIENLGARGVETYYLGPTNDPYLT
                     QLAAAENRDSGHSMADVRRLLDRIYAGVRQDNSRKLAEAVQASLFRSLGKVNPALQDR
                     GVKAAPFIVLLDTEMPAILAEVSCLSNDEEARLLTKPLYRQYIAEALAAGIRSYADAT
                     AGTASPAAPPTSTAQAAENPGEKGT"
     gene            13819..14850
                     /locus_tag="VEL74_03425"
     CDS             13819..14850
                     /locus_tag="VEL74_03425"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HYG61607.1"
                     /translation="MSDKQQVLHVGIDLGTSRSSISSSNGQRHVVESYVGWPLDMVAR
                     KVLKKPVLIGHEALENRSMLDLHRPLEQGLIKEGSEKDEAAVRELLWHLLSLVGVEKD
                     SKNGTRVRAVVGVPAEALRVNKQHVRKALEGAVDSLMIVSEPFAVAYGLEALIHSLVI
                     DSGAGTTDLCVMNGRLPTEDDQRTLPQAGDFVDEQLERLVRTRHPEASFSIHMVREWK
                     EEGSFVGTTKTPVMVTVPVKGKPTQIDITQEMRQACEALLPPMVESMLDLIAGVDPEY
                     QERVRNNVVLSGGTGLIRGLGPKLEQELQAVGGGKVRVVKDPIFVGSDGGLAIALDAP
                     ENDWEKLSA"
     gene            complement(14935..15777)
                     /locus_tag="VEL74_03430"
     CDS             complement(14935..15777)
                     /locus_tag="VEL74_03430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HYG61608.1"
                     /translation="MRNPLRLLAPAVLLILLPALSLAQAPAAPAAPAAPATAPIPAPP
                     DVAAPPADAQTTASGLAFKILTPGTGTERPVLGDGVEIHYTGWTADGKMIDSSVARGK
                     PLSLALESASPGWKEALQLMVPGEKRRLWIPESLSSGRGARGPLVFDVELVRLLRVPP
                     VPADVAAPPADAERGKSGLASKVLTPGTGTRKPSGRSRVRVHYTGWTTDGKMFDSSFQ
                     RGTPSTFSLDEVIAGWTQGLQLMVEGETRRLWVPKNLAYRGEPGKPQGMLVFDIELVE
                     IVRQ"
     gene            complement(15820..16383)
                     /locus_tag="VEL74_03435"
     CDS             complement(15820..16383)
                     /locus_tag="VEL74_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61609.1"
                     /translation="MLKIRFNLGAGSFMADYDATVRRDVAAFARRYFAGTITLDTFLR
                     YCGGNDDPLIRALSDALVHEPPREGFLGLRERWWRSRFWRPVERLIAELDKGAEGQVP
                     VERVYPRISLGGLIVLAAVVLWAGLFAARNLEQLLSDMYRSGALSFWDALWRATFAAT
                     MALITAAGLESWIHRLQLYRTRKLSRD"
     gene            16473..17234
                     /locus_tag="VEL74_03440"
     CDS             16473..17234
                     /locus_tag="VEL74_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61610.1"
                     /translation="MNRRELLKSLAGSVASYALFRTVFTRDAFAAAVQPVTSSWLKGL
                     HEMSLDLKTGTITPGQWQARIQELFNRVDAAELIAAIDFGKLTQGFEYPDQGVNTKWV
                     KFPKLAGLPADLAFYSKIFGMKRDRAIIPHGHRNMVSCHYVLQGELRLRHYDKVEEDG
                     THMVIEPTVDQAAPVGSHSSISDERNNIHWLRAATETAFTYDVLIVDLQGKKWEVDNI
                     DPHGAEKIAGGRLRVRKLGLDEALRKYGHEAHHHT"
     gene            complement(17284..17475)
                     /locus_tag="VEL74_03445"
     CDS             complement(17284..17475)
                     /locus_tag="VEL74_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61611.1"
                     /translation="MKLRLMLVLAVVTLTLGLSAPAPADPPITPQQCAEICETVRCAA
                     PTTCGLYVGAKGQVLCGCH"
BASE COUNT         2541 a         6223 c         6460 g         2473 t
ORIGIN      
        1 gtccccgagc tcggccgcca caccgcggcg gtgctggccg aggtgggggc acccgaggcg
       61 ggcctctccg cccggcgccg cgccgccgcg ggcatcggcc cccgcttcag ctggaagcgc
      121 tggctgctgc ggctggtgcc cggcctgccc agacagcggc ccggatagcg gcgcccggtt
      181 gacctcccct ctccctggcg gtacggtttc tcctctttct tcccagggcc gacggccgaa
      241 aggcgcggcc cgccgccggc atgtcaaatc gggggggacc gtggacgaag tgagctcgcc
      301 agtactgacc gaagagctgc gggaggccca tcgcagcctc tccgaggtgg acctcaggtt
      361 ccgggagtac gcctcggcga gcccgcagct cctgagctac gccgaattcc gggtgccgga
      421 gtcgtaccgg gagatgctga gcttccgtct ccagccctgg ccgaccttca tcgaccggca
      481 caagcgggag gagatcgcca gggtctccgt cgccgtgagc gacctcgtca agagcgtgcc
      541 gcaccgcgtc ttcggaggcg accccgtggc cgtcagccgc ttctatgggt tggcttctcc
      601 ggaggaggcg gaggacctgc tgtccgagcc caacggcatc gccgagatcg tggcgcgcgg
      661 cgacctgatc gccaccgcgg acggcttcaa gtgcatcgag ttcaacttcg cgacacggct
      721 cgggggttgg gagtcgacca tcctgtccag cctccacctt tcttcgcccg cgatcatccg
      781 gttcctccag caggagggga tccggcccgc ggtgactccc accacccgct gcttcttccg
      841 tcacatcctc gaggaggtcg agcgccgtgg gctcgcccgc ggggtgggcg tcaacatcgc
      901 cttcgcgttc cacgagcggt tggcgcgcac ccccatcatc atccaggggg tggcgcgcct
      961 cggagccgag tgggaccagg tctgccggga gaccgggcgc cagggcaagg gacggctcct
     1021 ggccgcgacc tggcaggagc tgagccccgg gtcttccggc caggggctgt cctgccgcgg
     1081 cctgccggtc cacgccgtcg tcgagcagac gaccgagccg gtgccggagc cggtctaccg
     1141 gtgcttcaag gaggggggga cctgcctgtt caacacaccg gtgacgacga tcctgaccag
     1201 caagatgaac gtcgccctgc tgtccgagca cgaaggctcc ggccggttca gccccgagga
     1261 gagggaggtc atccgccggc acatcccctg gagccgccgg gtggtccgcg acaccgtcga
     1321 tttccgcggc gagacggcct acctgcccga cctgctggcg gcgcgcaagg aggacatggt
     1381 cctcaaggag gcgcagaacc tcgggggcaa gggggtggcg gtcggccggt tcacggcggc
     1441 ggaccgctgg gcggacctct gccgctcggc gctggagaac ggcgagtgga tcgtccagga
     1501 gtacctcgaa tcgacctcct acctcttcca gagcggcgac cacggctgca cgccgcacaa
     1561 cgtcatctgg gggccgttcg tctttgggag cacctacgcc gggctcatcc tgaggatgca
     1621 gtcgaaggcc gtgccgggag tggtcaacct cagccagacg gccaccgagg gggtggtctt
     1681 cgaggtgtga gcccgctcgt gtaggatgcg ggcctctctc tttggaggtc catgcgatga
     1741 ggttcggcat cccgacactg gcgctgctcg ccatccccac cctgctcctg gcccaggcac
     1801 ccgctccccg gcccgcggcc cctgggaccc ctgaaacccc tgaggtcatc gccctccgcg
     1861 cggcgcgcct cttcgacggc aagggggaca cggccgtccg caacggcgtg gtgatcatcg
     1921 aggggagcac catcaaggcg gtgggctccg gcatcccggt gccggcgggg gcgcgggtga
     1981 tcgacctggg ggacgcgacg ctgctgcccg gcctgatcga cgcccacacc cacatcaccg
     2041 gcgaatcgtc cgacaactgg ctgcaggaca cggtctccgg cctgcgccgc gacgtgcccg
     2101 agagcgccat ccgcagcacc gagttcgccc gccgcaccct gatggccggc ttcaccacgg
     2161 tgcgcgacgt ggggagcggc gagtacgtcg acgtcggcct ccgcaactcg atccgcaacg
     2221 gcatcgttcc cgggccgcgc atcctcgccg ccgtccactc cctgggggcg cgcggcggcc
     2281 actgcgacga gagcggctat ccttaccagc gcttcgggca cgagtcgggc atcgaggacg
     2341 gcatcgccag cggccccgac cagttccggg actccgtgcg cttccagatc aagtacggcg
     2401 ccgacgtgat caaggtctgc gccacgggcg gcgtgctgtc gctgaacgac gaggtggata
     2461 cgccgcaggt cacccaggag gagatgaacg ccatcgtcga ggaggcgcac cgcctgcgca
     2521 agaaggcggc ggcccatgcc cacggcgcgg agggggccaa ggtggccatc cgcgccggca
     2581 tcgactccat cgagcacggc tcgttcctcg acgacgaggc gctgcggatg atgaagcagc
     2641 gcggcaccta cctggtgccg acgctgctcg cgggcgagta cgtggcgggc aaggccgccg
     2701 tgcgccagta tccgccggag atcgcggaga aggcccgggc ggcggtggcg gcgcgctcgg
     2761 ccaccttccg caaggccctg cagatcgggg tgaagatcgc cttcggcacc gactcggcgg
     2821 tgagcccgca cggcctcaac gcccaggagt tcgccctgct ggtcgaccac gggatgagcc
     2881 cggccgccgc cctgcgctcc gccacctccg ccgccgccga cctgctcggc ctggcgaaga
     2941 cgatcggcac cctggagccc ggcaaggagg ccgacatcgt cgccgtcccg ggcgatccgc
     3001 tggccgacat ccgggcgatg gagaaggtac ggttcgtgat gaagggcggg agggtgttca
     3061 aggacgagag tcagcccggc cccgcgggga agtagcgctt cgccagagcc gtcaggaaga
     3121 tctcgaagtc gccgaggcgg tgctcgagga catccgcgac ctcctccgga tcgacgtcct
     3181 catagagatg gacgatccgg ttccggaagg aggtcatcct ccggaaggtc tccttgtgct
     3241 cggccggcag gatgccatgc tcgacgagca ggtccatcga gtcgcggtac cggcggggca
     3301 cgccgagccc ctcgcgggcg atgatgtggt ttgccgcgtc gatcatcgcc tcgatggcca
     3361 cctggagatt tcgtagcgcg gcatcctgca ggagcggatc cgccaggaag gcctccctgc
     3421 cgcgggcccg gatctcttcg agccgctgca cggcgccgcg gatgaactgc cgcttgttga
     3481 ggagcttctc gggatcaagc gtcacggccg tagcgctcgg taaatgcctg gtcgtagtcg
     3541 cgcagaaact gctcgtggtc gacgatgaag tccccgtgct cgttgaggac cctctcggtg
     3601 aagtcggcga gagccacggg gtcgttgcag acgagctgcc ggccggtctc gagaacgcgg
     3661 aactggaaga tcaccggtga gcggttgagg acggtcaccg aggcgtcgtc ctcgtggagg
     3721 atctgctgga tgtcggcggc gatgccgagc tcctcccgga aagccgggac cttgtccgga
     3781 cggaagacaa gggccaggtc gaggtcgctc agcggggtct ggtagggggt gccgtaggag
     3841 ccgtggatcc aggcggccgc cagcccatcg agcccttcga ggtaggcccg gaggtccggc
     3901 aggtaggctt ccacgtcgat cggcggctgc ccctcgcgcc atctcatcgc atgctccctc
     3961 cgcgccagca aggtaccacg cccggccggc gccgtcaccg tggcgggggc gtccctggcg
     4021 gcgtcccccg cagtgtcctc cgcgcgacgg agagcacggc ggcgaccagg tcgcggggct
     4081 cgacgggctt cgcaaggtgc atctggaagc cggcttcgag ggcttggcgg cggtcccccg
     4141 cgccggcctg ggcggtgagg gcgatggcgg ggaggtcgcc caggttgccg tccagggcgc
     4201 gtacccggcg gatcagggca tagccgtcct cgcccggcat ggcgatgtcg gacaccagca
     4261 cgtccggctg gaagcggggg agggcggcca gcgcttcggg ggtggaggcc gccgcctcca
     4321 cttcggcgcc gaaccgcgcg agcagcgccg gcagcacctc gcgggtgtcg tcctcgtcgt
     4381 ccaccagcag gatcttgagc ccctggaggt ccaggccgtc gccgtcggcg ggcaccgcgg
     4441 cccgctccgg cgcctccggc ccggcgccga gccgcagctc gggtgccacg ggcaggaaga
     4501 cggtgaacac cgccccccgg ccctcgccgt cgctctccgc ctccaccgtg ccgccgtgca
     4561 gctccaccag gtggcgcacg atcgccagcc cgaggccgag gccgccgtgg gtccgcgtcg
     4621 tggtgctgtc cgcctggcgg aagcggtcga agacgtgctg catgaagtgg ggcgagatgc
     4681 cgatgccggt gtcgcgcacc cggatggcgg tgtacgcgtc ccccgccagg aggctcacct
     4741 cgaccgtccc ccccttgggg gtgaacttga cggcgttcga cagcaggttc cacaccacct
     4801 gctgcaggcg gttcgggtcg cccgccgcca gcgccgccag cggatcgagc cggctctcga
     4861 tccggatccc cttggcgtcc gccgccggcc gcacggagtc gagcgccgcg tcgatgatct
     4921 ggatgaggtt gagcggcacc acctggagga ggagcttgcc ggtgataatg cgcgagacgt
     4981 ccagcaggtc gtcgatgatc tgcccctggg cgcgggcgtt gcgctcgatg gtggcgaggc
     5041 cgcggcctgc ctcgtcgccg gtcagcctgc cgttcgacag cagccagacc cagctcagga
     5101 tggcgttgag cggcgtgcgc agctcgtggg agacggtgct caggaactcg tccttgatgc
     5161 ggttcgcctc ctcggccttg cggcgcgcct cctgctcgct ctcgaagagc acccgcagct
     5221 cggcctcgga gcggcgcagg gcggcggtct gctcctccac tcgccgctcc agctccgtgg
     5281 catggcccct cagctcgccc tccttgtcgg cgagggcgtc ggccatggcg tcgaaggcgg
     5341 cggccaggcg ccccagctcg tcgtcccggt ccagccgcac gcggcggctc atgtcccccc
     5401 gggcgagggc gttggcggcc tggccgacga ggcgtagcgg gcgggtcgcc cagccggccg
     5461 ccgacagggc gagggcgatg gccagcaggc cggtgaccgc cgcgaacacc aggctgcgcc
     5521 gcgtctgctg gctgaccgag gcgaggatcg ccggctcggg gctgaagatg ccgtagagcc
     5581 agcgcgtcct cggcaacggc accaccgcgg cgtgcagcgg caccccgccg gggccttcga
     5641 gctccaggta ggggtcgcgg ccgcggtcgt agagcagcca gcgggagcgc tccaccagcc
     5701 cggcggccgg ccgcggccgg gccagcaggg ccgcggtggc ggggccgaag cggctctcgc
     5761 cgatcaggcg ggcggcgacg tccgccggca gcggctccag cgggtggaag cgcagggagg
     5821 ggtgcgtcgg atggctgagg cggatgccgt cggcgttcca cagcacgccg aactcgcccc
     5881 ggccggcgaa gtcggtgtcg gcgcggatca gctcgtcgat ccgcgccagc cgcacccgcg
     5941 ccaccgccac cccggcgatc cgccccgcct ccccgcggat gggggtggcg gcgtgcaggt
     6001 agacctcgcg gtcggccggg tcccagcgcg gctcgtcgac cagggtgttg ccggcggcgg
     6061 cgtcccggaa gtagggggcg gagacatagc tgcggccgcg gaagcggggc tcgctggcga
     6121 ggcgcacggt gccgccgggc tccatcagat agagggcgtc gagcttctgg gcgtcgcgga
     6181 tctggcgcag gatgccctcc acctcccggt cgagggcgtg ccggcgccag ccggtgaaga
     6241 acgagaccgt ctgcggggcg gtggcggcca ccgacaggtc ggcgcggagg tcgtccagca
     6301 ggttttccag gatggacgcc gtgttgcgcg cctgctggag gttctgggcc cgggcgtcgc
     6361 ggatcaggcc ggcgcgcgcg accgcctcgt tgaacagcgt gacggcggtg agggggacga
     6421 gggccaggcc gcctaggaga agggagagct tggccgccca gggccagtcg cgccagcgcc
     6481 agcgccggct cacggctcga ggcgcaccag gctgaagtcg ttgttcggga cgagcccgcg
     6541 ggcctcggcg tgcggcgtgc cgtggacctg ccggcgcatg acggcgatgt agaccgactc
     6601 ggcgagcggc gcgatgggga ggtcgcgcgc caggatgcgc tgcgcccgga aataggccgc
     6661 cgcgcgccgc ggcaggtcgg cggcgcgcga cccctcggcc acggcggcgt cgagctccgg
     6721 gctggagtag cccatgaact gggtcgggcc gcgggagccg aagcggaggt tgaggttctc
     6781 cggatcgggg ccctggttgc cccccatgat cccgaggtcg aagtcgtgat tgttgaggag
     6841 gcgcggcgcc cactccgcca gggggaggag cacgggccgg atctcgaccc ccacctcccg
     6901 gagctgcgcg gcgaggacgc ggaccatgtc cgggatcggg aggatgtccg gcgccaccat
     6961 gtcccatcgg acgcgcacgc cgtccggccc ggggcgcagg cccgcctcgt cgagcagggc
     7021 gcgggcgcgt tccggatcgt aatccggcac ccgcgcctgc gcgtcgtagg cccaggccac
     7081 ggccggggtg tagaagccga gcgccggcgc gccatagccg gcgaacgcct tctcgagcag
     7141 ggctttgcgg tcgatggcgc ggttgaccgc ctcgcgtacc cggcggtcgg ccagggggcc
     7201 gcggcgcagg ttgaagccgc agtagaagcg gctgtcgctg ggacgggtga ggatcgcgag
     7261 gtcgggcgac cgcgccagct ccggcagccg gtccaccggc ggccggacgc cgaagtagtc
     7321 cgcctggccg gcgaggagca ggtccacccc ctcgccggcg gagcgcagca ggtagaccac
     7381 gcggtccaga taggggccgg gccggaagta ggcgcggttg gcggccaggg tgatgctccg
     7441 cccgggctcc cagctctcga cgcggaacgg gccggtgccg accggcagct cgccgggctg
     7501 gaacccatgc gccccccgcg cctcctggcg gtggcggggc aggatgtagg tgccgtacca
     7561 ggcgagcatg gagaggaacg gcgaccaggg ctcgcgcagg cggatgacga cggtgcgcgg
     7621 gtcgggggtc tcgatggcgg cgatgcgctg gaagacctcg gtggcgaacg acggctcgcg
     7681 gcccagggcg gtcaggctcc agcgcacgtc gtccgcgctg aaggggtgcc cgtcgtgcca
     7741 gcggacgccg tcgcgcagcc ggaaggtgta gacgcggccg tcgggcgacg tcgtccagct
     7801 ctcggcgagc tgcggcagga gccgggcgtc gacgtccagc gtcaccagct tgctgaagac
     7861 gctgggggcg accaccaggg ccgcctcgtc ccgcatggag ttggggtcga ggacccgcgg
     7921 ctcgagcggc aggatgacgc ggagctcgcc gccccctccc ttgcgctcgg gctggcgcgc
     7981 cggccgcggc tccggcgtct cccggccgca cccggcgaag gacagcagga gcgccgccgc
     8041 cagggtcagg ctcttgagat ggttgttcat ggtcgaagcg gcgccctcgc cgccgtatca
     8101 tagggaaaag ccagcgcctc cgccttcccg cgggacgtcc agaggggcct ccgtgtgacg
     8161 tccggggctc ggcgtccgta taaactctgg ggggctgtcc gctcctctgg agccgcgctc
     8221 cggccaccga cgaggtcttc catgaagatg ttcctgaaat cgatcgccgc cttcgtgttc
     8281 ctcgccctgg tcatctgggc gatccgttcc gactcgctgg aagagagggg gaacctgggg
     8341 gccgcccccc tggccggcgc cgcggtcccc gcgggggacc cagcctggcg ccgcgcgccg
     8401 ctgtgggacg acggcaagtc cgagttctgc gcctacgagg ccacttggcc gcgctacggc
     8461 caccgcttcg aggggcgcgc cctgctggtg ctggtcaagg agccctgggc gccggacctg
     8521 aacgtcaagg ccgacaaccc ccgtcccggc ggcttcgagg tgctcaagct caaccacgtc
     8581 cgcgacgtgc ccaccggcat ctactcgtac caccagatgg cgagcgtcta ctcgcaccgc
     8641 gagtcggggg cgctggtgaa gctcgccgcc accagcgccg aagcctgcgg catctcgggc
     8701 gccgagatgg tgggggggcg gctcgccacc cgctcctact tcgacggcca gggggaccgc
     8761 tcgctcccct ggccggcgga ggcgctgccg gaggacggcc tgcccgcagc tctccgcgat
     8821 tacgtgaccg gcccggtgcc ggaccacctc tcggtgttcc cgtcgctcat ggcctccagg
     8881 ctgccggagc tggcgccctc catctataag gtggagaggc gcgcggcggg tgaggtggag
     8941 acgccggccg gcgcctctcc gggggtggag atccgcctga cccggggcaa gtcctggctc
     9001 tcctacactt tcgaggccga ggcgccgcac cgtctgctcc gcctccagag ccacgacggc
     9061 accgagtacc gtctcgccaa gtgcgaccgg atcgcctact ggcagatgca caacccgggc
     9121 gacgagtcct ggctgccgga gtcgacgcgg taggaccctt caccccccgg cgccctctcg
     9181 cgcctccccg cccctcctcc tgggagaggg ggaggcggtg cctgtcgccg gcatggcgtt
     9241 ccctttcatc ggcacggctc tgcccgccat cggcgtggcg gcgccggtcg ttggcccgcc
     9301 gcggcccgcc gtcggcaccg cgcggccggc cgtcggcgcg gcgcggcctg tcctcggcgc
     9361 gttggtgcct gtcatgggcg cggcgcggcc tgtcgtcggc gcggcgcggc ctgtcatggg
     9421 gaacggtctg ccggggaccg gcacggcatt gctcatggga ggcccgcctc cccctctccc
     9481 ggggagggag agaggggggg ccggaggtga gggctcttac aggcgggtca ggacgtcggc
     9541 gatccgctcg acggcccagt cgatctcgct ctgctggatg gtgagcggcg gcgcgacgcg
     9601 gatcacgttg tcgtgcgtct ccttgcagag gaggccgtcc tgcttgagcg cctcgcagaa
     9661 gcggcgggcg ccgccggcct cggggtgcag ctcgatgccg atccacagcc ccttgccgcg
     9721 cacctccttg acgtgcttcg actcgatggc gcgcaggcgc tcgatgaagt aggggccgag
     9781 cttggcggag ttctcgacca tcccctcctc gaccagcacg cgcagggcgg cgcggccgat
     9841 ggcggcggcc atcgggttgc cgccgaaggt cgagccgtgg tcgcccggct tgaagacgcc
     9901 catcaccggg tcgttcgcca ggatgcccga gatcgggtag aagccgcccg agagcgcctt
     9961 gccgacgatg accatgtcgg gctggatccc ctcgtgctgg aagcagaaca gcttgccggt
    10021 gcggccgagg ccggactgga tctcgtcggc gatcagcagc gcgttgtgct cgcgggtgat
    10081 ctcgcgcagc gccttcatgt agccgtccgg cggcatcacg atgccggcct cgccctggat
    10141 gggctccacc agcacggcgg cgacgttggg gttcatcgcc tgggccacgg cgtcggcgtc
    10201 accgtagggg aggatgcgga agccgggggc gaaggggccg aagccggact tgtactgctc
    10261 ctcggtcgag aagccgacga tggtcgaggt gcggccgtgg aagttgttgg cgaagaccag
    10321 gatctcggcc tgcccgtcgg ggatcccctt gacggtgtag ccccacttgc gggccgcctt
    10381 gatggcggtc tcgaccgcct cggcgccggt gttcatgggc aggaacttgt cgaagccgcc
    10441 gagctcgcag atctgcttgt agaaggggcc gagctggtcg ctgcggaagg cgcgcgaggc
    10501 cagcgtcacg cggctcgcct gctccagcat cgcctgcagg atcccggggt ggcagtgccc
    10561 ctggttgacc gccgagtagg cggcgaggaa gtcgaggtac ttgcggccct cgacgtccca
    10621 cacccagacc ccctgggcgc ggctgaccac cacgtcgagc gggtggtagt tgtgcgcgcc
    10681 gtagcggtcc tcgagcgcga tgtagtcggc ggtcgtcagg gcggacgtgt cttccagcac
    10741 ggtcgtgctc atggttcctc cgatagctgc aagggtagtg acccgtaggt agagagtcgg
    10801 ctgtggccga tcggtgacga tcgtacggcg atcggttcgg aatgtctttt tatcacgaaa
    10861 gaggcggcag gccaagggcc tgctatgatg cggggctcag cgggctcatc acactatggt
    10921 cgattactcc aacatccctc gggactcccg gcgcgtgccg ctggagaccc gcgtgcagtt
    10981 caagttcgat cgcttcttcg ggttcctcag cgagtactcg tccaacatct cgccgggcgg
    11041 catgttcatc aacacccagc agccgcagcc gccgggcacg gtgctggacg tggagttcag
    11101 cctcggcgag gacggcttcc agctcatcca ggcgcgcggc gaggtgatct gggtacgcaa
    11161 ggaggaccag ggccccggcc ggccggccgg catgggcctc cggttcctcg agctgagcga
    11221 cggcagcaag gagctcatct atcggatggt ggacaactac gtccaggagg ggggcgtccc
    11281 cttcgacctg tcgctggtgc cggacgagat gccggccgcc gccaaggccg ccttcccggg
    11341 cgccgcggcc gccgcggcgt ccctgccccg gacgcccccc gccaacgacg acgagccctt
    11401 cgacctgtcg gcggacccgt tccagctcga ggacatggag cctgccgtcc agccgcggca
    11461 ggaggtcctg gtcttccccg acatcgacac ccccgcgttc ccggagccgg aggccgatcc
    11521 cctgcccgac ctcgactccc tggccccccc gcccggcggc tgggctccgc agccggcgcc
    11581 ggaggagagg cccggagaga tgccgcggga gctgctggag ccgctgccgg aacccgcacc
    11641 ctgggcggcg gcggagccgc agcccgaggt gccggccgag ccgtcggccc ctctcctcgc
    11701 gcgcgaggag cccgagccgg cgcgctggac ctcggcgccg ccccccctgg agccggagag
    11761 ccagagctgg tcgggggtcc cgcgggaccc ggaggccgcc ccgccgccgg tctacctgcc
    11821 cgagccgccg ccgccgcgcg aggcgccgac gccgctggtc cactacgccg gcagcgcccg
    11881 ggccaccgag ccgcgccgca gctccgtgcc gtggctgatc gtgctgctgg tggcggtcgg
    11941 tgccctgacc gcgttcgtgc tgatgaacga gcagatcatg gactgggccg gcctcggcag
    12001 cggcgagatc accgcggcga cgcccggccg gccgctcccg cgcccccgtg cccgcgccac
    12061 ggtccccgcc ccgggcggcc cgcccgcggc cacggtgacg gaggggacgc cggccgcccc
    12121 ggtggtcatc gaggaggctc ccggcgccac ccagccggcg gcgcccgcgc cgtccgcgcc
    12181 gggcgcgcag accgccgcac cggcaccggt ggccgccgct cccgctcccg ctaccggtcc
    12241 gccggccacc gtcatccagc ggatcacctt cgagcggctg cccaccggga ccgagatcgt
    12301 cctctggggc aacggcgcca tccgcctggg ctcctacacc cggctgcgca tcgacggcaa
    12361 cccggcgcgc gaggtgatca agctcaaggg catccgccag cgctacccct cgccgcgggt
    12421 cggggtgggg acgcccgagg tcctgcaggt gcgggtcggc caccatccgg gcgacgagct
    12481 gcacgtcgtg ctcgacctca ccggcccgcg cgtccaggcg gcgggcttcc aggaagacgg
    12541 caaccggctg cgggtgctgg tccagcggcc ttagaagcgg ccggacggga tcactcggcg
    12601 ttgtagtaga atgcgcgcct cgacaagacg cgctgaacga gccgtagacc gtcgtgggag
    12661 tatgcaggtg ttggagctcc ccatcctgcc agaggtttcg aacgtccatc ccctttgcgc
    12721 ccggagatcc cgcaggtgac gtctgacgtg catcgcgacg tcagccgggc gaagcggcag
    12781 atgctgcgcg agctggtccg cgagaacgtg gacctcaagc aggggcggtt gccgcgccgc
    12841 ctgcgcgccc ggccgagcgt ctggcaccgg ctgccgccgg cggcccgcat cgccatcgcc
    12901 gtcgtcctga tctgcggcgt gttcggctcc tcttggctgc tgtgggcgct gagcgccgag
    12961 ccggtcaacc tgacgatcat ggcgggccgg ccgcagggat cgccggtggc ccagccggcg
    13021 gtctccacgg agccccgtcc cggcccggcg gtcgacgcct cggtcttccc cctggcggtc
    13081 cggcgggtgg tcatcgaccc cggccacggc ggcgacagca acggcacccg cgcgccgctc
    13141 ggcctggtgg agaaggagct ggcgctggag atctcccagc ggctgcggcg cctgctggag
    13201 cgcgacggct tccaggtgct gatgacccgc caggacgacc gggccgtgac cctcgaccag
    13261 cgcgccgccg tcgccaacga ggcgggggcc gacatcttcg tctcgatcca cctcaactgg
    13321 atcgagaacc tgggggcgcg cggcgtcgaa acctactacc tcggccccac caacgatccc
    13381 tatctcacgc agttggcggc ggcggagaac cgcgactccg gccactcgat ggccgacgtc
    13441 cggcgtctcc ttgaccgcat ctacgccggc gtccggcagg acaactcgcg caagctcgcc
    13501 gaggcggtgc aggcgtcgct cttccgttcc ctgggcaagg tcaacccggc cctccaggac
    13561 cgcggggtca aggcggcgcc gttcatcgtg ctgctcgaca ccgagatgcc ggccatcctc
    13621 gccgaggtct cctgcctctc gaacgacgag gaggcccggc tcctgaccaa acccctctac
    13681 cggcaataca tcgccgaggc cctggcggcg gggatccgct cctacgcgga cgcgacggcg
    13741 gggacggcct ccccggcggc cccgccgact tcgactgccc aggccgctga gaaccctggc
    13801 gagaaaggaa catgagacat gagtgacaag cagcaggtcc tccacgtggg gatcgacctg
    13861 gggacctccc gcagctcgat ttcgtcgtcc aacgggcagc ggcacgtggt ggagagctac
    13921 gtcggctggc cgctcgacat ggtggcccgc aaggtgttga agaagccggt gctcatcggc
    13981 cacgaggcgc tggagaaccg ctccatgctc gacctccacc ggccgctgga gcaggggctg
    14041 atcaaggaag ggtcggagaa ggacgaggcg gcggtgcgcg agctgctctg gcacctgctg
    14101 agcctcgtcg gggtggagaa ggacagcaag aacggcaccc gggtgcgcgc cgtggtgggc
    14161 gtgccggcgg aggcgctgcg ggtcaacaag cagcacgtgc gcaaggcgct cgaaggcgcg
    14221 gtcgacagcc tgatgatcgt ctccgagccg ttcgccgtgg cctacggcct cgaggcgctg
    14281 atccactcgc tggtgatcga ctcgggcgcc ggcaccaccg acctctgcgt gatgaacggc
    14341 cgcctgccca ccgaggacga ccagcggacg ctgccgcagg ccggcgactt cgtcgacgag
    14401 cagctcgagc gcctggtgcg cacccgccac ccggaggcga gcttctccat ccacatggtg
    14461 cgcgagtgga aggaggaggg gagcttcgtg gggaccacca agacgccggt gatggtcacc
    14521 gtgccggtga agggcaagcc gacgcagatc gacatcaccc aggagatgcg ccaggcctgc
    14581 gaggcgctgc tgccgccgat ggtcgagtcg atgctcgacc tgatcgccgg ggtggacccc
    14641 gagtaccagg agcgggtgcg caacaacgtc gtcctctccg gcggcaccgg cctgatccgc
    14701 ggcctgggcc cgaagctgga gcaggagctc caggcggtgg gcggcggcaa ggtccgcgtc
    14761 gtcaaggacc ccatcttcgt cggctccgac ggcggcctcg ccatcgccct cgacgcgccg
    14821 gagaacgact gggagaagct gtcggcgtag gcggcacgga cggcacggac catcacggac
    14881 tgacacggac gatcacggac cggcggggga gggggaggtg gtgcctcccc ctgcctactg
    14941 ccgcacgatc tcgaccagct cgatgtcgaa gaccagcatc ccctggggct ttccgggctc
    15001 gccgcggtag gcgaggttct tggggaccca gaggcgccgc gtctccccct cgaccatgag
    15061 ctgcaacccc tgggtccagc cggcgatcac ctcgtccagc gagaaggtgg acggcgtgcc
    15121 gcgctggaac gacgagtcga acatcttgcc gtcggtcgtc cagcccgtgt agtggacccg
    15181 cacccggctg cggccggacg gcttgcgcgt gccggtcccc ggcgtcagga ccttggaggc
    15241 cagccccgac ttcccccgtt cggcgtcggc cggcggggcg gcgacgtcgg ccggcaccgg
    15301 cggcacccgc aggaggcgga ccagctcgac gtcgaacacc agcggtcccc gggcaccccg
    15361 gcccgaggag agcgactccg ggatccagag gcgccgcttc tcccccggca ccatgagctg
    15421 cagcgcctcc ttccaccccg ggctggcgga ttcgagggcg aggctcaacg gcttgccgcg
    15481 cgccaccgac gagtcgatca tcttgccgtc cgcggtccag cccgtgtagt ggatctcgac
    15541 gccgtccccg aggaccgggc gctccgttcc cgtgcccggg gtgaggatct tgaaggcgag
    15601 gccgcttgcc gtggtctggg cgtcggccgg cggggcggcg acgtccggtg gcgcggggat
    15661 cggcgctgtc gccggggcgg ccggggcggc cggcgcggcc ggcgcctggg ccagggagag
    15721 agcgggcagg aggatgagga gcacggcggg cgcgagcaga cgcaggggat ttctcatgct
    15781 ccccaccgta tcgcgccaac cgccgggaga caacccgttt cagtcgcgcg acagcttccg
    15841 ggtccggtag agctgcagcc ggtggatcca gctctcgagg cccgccgcgg tgatcagggc
    15901 catggtggcg gcgaaggtcg cccgccagag cgcgtcccag aaagagaggg cgccgctccg
    15961 gtacatgtcg ctcaggagct gctccaggtt ccgcgccgcg aagaggccgg cccacagcac
    16021 gacggccgcg aggacgatca atccccccag gctgatccgg ggatagacgc gctccaccgg
    16081 cacctgccct tcggccccct tgtcgagctc ggcgatgagg cgctccaccg gccgccagaa
    16141 ccgggaccgc caccagcgct cccgcaggcc caggaacccc tccctcgggg gctcgtggac
    16201 gagggcgtcc gacagggccc ggatcaacgg gtcgtcgttg cctccgcaat accggaggaa
    16261 ggtgtcgagg gtgatcgtcc cggcgaagta gcgccgcgcg aaggcagcga cgtcccgccg
    16321 caccgtagcg tcgtagtcag ccatgaaact tcccgctccc aggttgaatc gaatcttgag
    16381 catgagtata tctccctgag tggccgtggg gaagagggtt cgggagcccg gggggtacgg
    16441 tatgatttca tcctcttatc gggagcgatg cgatgaaccg gcgggagctg ttgaaatctc
    16501 tggcggggag cgtcgcctcc tacgcgctgt tccggacggt cttcacgcgc gacgccttcg
    16561 ccgccgcggt ccagccggtc acctcctcct ggttgaaggg gctccacgag atgagcctgg
    16621 acctcaagac cgggacgatc acgcccggcc agtggcaggc caggatccag gagctgttca
    16681 accgggtcga cgccgcggag ctgatcgccg cgatcgactt cggcaagctg acccagggct
    16741 tcgagtaccc cgaccagggc gtcaacacca agtgggtgaa gttccccaag ctcgccgggc
    16801 tgccggcgga cctcgccttc tacagcaaga tcttcggcat gaagcgggac cgggcgatca
    16861 tcccgcacgg ccaccgcaac atggtctcct gccactacgt gctccagggc gagctgcggc
    16921 tccggcacta cgacaaggtg gaggaggacg gcacccacat ggtcatcgag ccgacggtcg
    16981 accaggccgc tccggtcggc agccactcgt cgatctcgga cgagaggaac aacatccact
    17041 ggctgcgggc ggccaccgag accgccttca cctatgacgt gctgatcgtc gacctgcaag
    17101 gcaagaagtg ggaggtcgac aacatcgacc cccatggggc ggagaagatc gccggcggcc
    17161 gtctgcgggt ccggaagctc ggcctcgacg aggcgctccg gaagtacggg cacgaggcgc
    17221 accaccacac ctagagacgg gccggtcccc cgccggaacc ggcggaggcc ggcccgggat
    17281 cgctcagtgg cagccgcaga ggacctggcc cttggcgccg acgtagaggc cgcaggtggt
    17341 cggggcggcg cagcggaccg tctcgcagat ctcggcgcac tgctgcggcg tgatgggagg
    17401 atccgccggg gccggcgcgg agagtccgag ggtcagggtg acaacggcca gcacgagcat
    17461 caatcgaagc ttcatggggt actccacaag tggtctcgag gatcagtcct ctagcacttg
    17521 aaagtgcgct ggtcccatcg aagcgagccg ctctcccgct ccgttgactt ggcccgctcc
    17581 caggcttagc ctaatcgcga aaggagagaa gcgatgagaa agcgtgccaa gaagctggct
    17641 ctcagccggg agaccgtgcg ccatctgcaa ggctcccatc tccaccacat ccgcggc
//
LOCUS       DASPJI010000122         6178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_752563, whole genome shotgun sequence.
ACCESSION   DASPJI010000122 DASPJI010000000
VERSION     DASPJI010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6178)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752563"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>6178
                     /locus_tag="VEL74_03450"
     CDS             <1..>6178
                     /locus_tag="VEL74_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61612.1"
                     /translation="TLFMTLLSGVAALLERYTGQDDVVVGSPMAGRTRSELEGLIGFF
                     INTLALRLDLSGDPAFGRLLERVRAACLTAYDHQHVPLEKLVAELQPSRNLARSPLFQ
                     VLVQLHNAPRGPIDLPGLVLSPVTGEEDTAKFDLVLNGVERGEELGLVLGYSAELFER
                     ATIERLADHLATLLAGAVAEPACPLSDLPLLSGPERGQLLVGALPADPCLHQIFAARA
                     AASPGAVALVSDGDRLTYGELESRANQLARFLAARGIGPGVLVGLCLERSFDLVTAIL
                     GVLKAGGAYLPLDPDVPPARVAFMLEDSRVPLLLTQEGLRERLPAGAAGVVCLDGDEA
                     LIAQESPAPWSAPSSPDDPAYVIYTSGSTGRPKGVVVRHANVTRLFEATAPWFGFDGA
                     DVWTLFHSYAFDFSVWEIWGALLYGGRLVIVPYRVSRSPADFHALLAREKVTVLNQTP
                     SAFRQLSWIDQEREPGTLALRLVIFGGEALELQSLAPWLDRYGDARPRLVNMYGITET
                     TVHVTYREISRADLQAGRGSVIGRPIPDLTLHVLDRRGNLLPMGVPGELYVGGAGLAQ
                     GYLARPELTAERFVPDPFGSVPGARLYRSGDLARRLVDGDHEYLGRIDRQVKIRGFRI
                     ELGEIEAALASYPGVREVTVMAREGAASGERRLVAYVGAAAEPAPGALRAYLAARLPD
                     YMQPSAVVTLPSLPLTANGKVDHGALPDPNAVLAGHREPETELERALAGLFRDILKAG
                     RVGIDDDFFALGGSSITGAMLINRLQRELGEIVQVVVIFDHPTVGRLAAYLREQHPEG
                     VTRWLPQETPAVPVAAESAAGRVDEGMVERLRQLVPSFPASSASFSLAARGAKNPPAL
                     FVLAPPRSGTTLLRVMLGGHPRLFAPPELELLGFPTMADRRAAFAGRDSFWLEGLLRA
                     LKEMRGGSPEEVRAWAEEHERRGTPTLDLYALLQEELAGRWLVDKTPSYALDPAVLRR
                     AEEGFTAARYLHLVRHPCGMIRSFEEARLDQIFFRRAHPFTRRQLAELIWVVSHQNIL
                     SFLADVPADRQHTVRFEELLAAPERVLGGVCDFLGLEYHPDMARPYEGGSARMTDGLH
                     AASRMLGDVKFHQHSGVDAEVAERWRDGLREADLGSPARRVAAALGYPPGPRGLDRGA
                     RIERGTWGPGEPLPLSFAQERLWFLDQLHPGTATYNIPVALRLLGRLDVAGLRAALTE
                     VARRHSVLRTTFASADGRPVQVVQAAQPVPLPLVDLAGLPPDEREREALRVAGEASRR
                     PFDLARGPVLRAGLLRLGEAEHVALVGMHHIASDGWSVGVLIRELGALWPALGRSGSP
                     PLPEPAIQYADFALWQRAWLTPERLAANLGDWRERLADLPPLDLLPDRPRPPFASGRG
                     ATLSFPLPPHLGEALAVAGLRTGATLFMAALAGFMALLSRYAGQDDFAVGTPVAGRHH
                     AELEELIGFFVNTLVLRAGLEGSPGFDELVGRVRQTVLWAFGHQDVPFERLVEELQPQ
                     RDLARPPLFQVMFSLQNASSERLALPGLSLHSLQQAVTTAKFELTLSLAETVAGLAGE
                     IEYATDLFDRTTISRLAEHFAALLSAAVAAPSIRLTDLPLLGAAERQQLLLEWNDTQV
                     TRRGAAVRIHELFEEQAARRPQAPAVSGQGTTLSYAELEARANRLARRLRRLGVGPET
                     RVALCVDRSPEMVVALLGILKAGGAYVPLDPAHPAERLALVLDDSAPQALVTEERWLD
                     RLAFDGPRILCLDRDRESIHAEEAAALGAPTGGGAESLAYVIYTSGSTGRPKGVCLPH
                     GAVVNFLFAMVERLGLGADDVVPALTTLTFDIAGLEIYLPLALGGRVEVIGGEEGSDG
                     HRLAARLAAAGVTAMQATPATWRLLLDAGWSGREGFKALCGGEALPRSLAAELLARGA
                     GLWNLYGPTETAIWSATGSVAPAGEAVAVGLGRSIANTRLHVVDRRFEPVPPGVTGEL
                     LIAGAGMARGYWDRPDLTAERFLPDPWSKPGGARLYRTGDLVRHRADGELEFLGRVDH
                     QIKLRGFRIELGEIEVALLRHSQVSQAAVVVVGEGTDRRLVAC"
BASE COUNT          756 a         2172 c         2281 g          969 t
ORIGIN      
        1 gacgctgttc atgactctgc tctccggagt cgcggcgctg ctcgagcgct acaccgggca
       61 ggacgatgtg gtcgtcggct cgccgatggc ggggaggacc cgctccgagc tcgaagggct
      121 catcggcttc ttcatcaaca cgctcgccct gcgtctcgat ctctccggcg accccgcctt
      181 cggccgtctg ctggagcggg tgcgggcggc ctgcctgacc gcatacgacc accagcacgt
      241 gccgctcgag aagctggtcg ccgagctgca gccctcccgt aacctggcgc gctcgcccct
      301 gttccaggtc ctcgtccagc tccacaacgc gccgcggggc cctatcgacc taccggggct
      361 ggtgctgtcg ccggtgactg gcgaggagga caccgccaag ttcgacctgg tcctcaacgg
      421 cgtggaacga ggagaggagc tcggtctggt gctcggctac agcgccgagc tgttcgagcg
      481 ggccaccata gaacggctgg ccgatcatct cgccactctc ctcgccggcg cggtggccga
      541 gccggcctgc ccgctctccg acctgccgct gctgagcggt ccggagcgcg gccagctcct
      601 cgtcggcgca ctgcccgccg atccgtgcct gcaccagatc ttcgcggcgc gcgcggccgc
      661 cagtcccggg gcggtcgctc tggtcagcga cggcgatcgg ctcacctatg gcgagctcga
      721 gtcccgtgcc aaccaactgg cgcggttcct ggccgcccgc ggcatcggcc ccggagtcct
      781 ggtagggctc tgcctggagc gctccttcga cctggtgacc gccatcctcg gcgtgctcaa
      841 ggctggcggc gcctacctgc cgctcgaccc cgacgtgccg cccgcgcgcg tggcattcat
      901 gctggaggac agccgtgtgc ccctgctgct gacccaggag gggctgcgcg agcgcctgcc
      961 ggcgggcgcc gccggcgtgg tctgtctcga tggggacgag gcgctcatcg cacaggagag
     1021 cccggccccc tggtccgcgc cgtcctcgcc cgacgatccc gcctacgtca tctacacctc
     1081 cgggtcgacc ggcaggccca agggtgtggt cgtccgccac gccaacgtga cccggctgtt
     1141 cgaggccacc gcgccatggt tcggcttcga tggcgcggat gtgtggaccc tgttccactc
     1201 ctacgccttc gacttctcgg tctgggagat ctggggggcc ctgctctatg gtgggcggct
     1261 ggtgatcgtc ccctaccggg tcagccgttc gccggcggac ttccacgcgc tgctggcccg
     1321 cgagaaggtg acggtgctca atcagacccc gtcggccttc cgccagcttt cctggatcga
     1381 tcaggagaga gagcctggca ccctcgctct ccggctggtg atcttcggtg gcgaggcgct
     1441 cgagctgcag agcctggcgc cctggctcga tcgctacggg gatgcccgac cgcgcctggt
     1501 caacatgtac ggcatcaccg agaccacggt gcacgttacc taccgcgaga tctcgcgggc
     1561 cgacctgcag gccgggcgcg gcagcgtcat cggacggcct atcccggacc tgacgctgca
     1621 cgtcctagac cggcgaggga atctgctgcc catgggcgtt ccgggcgagc tctacgtcgg
     1681 tggggcaggg ttggcgcagg gatatctggc gcggccggag ctgaccgccg agcgcttcgt
     1741 ccccgatccg ttcggatccg tcccgggcgc ccgcctctat cgctcgggcg acctggcgcg
     1801 ccggctggtg gacggcgacc acgagtacct ggggaggatc gaccggcagg tcaagatccg
     1861 cggtttccgc atagagttgg gggagatcga agcggcgctc gcgtcctatc ccggcgtgcg
     1921 ggaggtgaca gtgatggcgc gggagggcgc ggcgagcggt gagcggcggc tggtcgccta
     1981 tgtcggggcg gcggccgagc cggcccccgg tgcgctacgc gcctacctgg cggcgcggct
     2041 gccggactac atgcagccgt cagccgtggt gacgctgccg tctctgcctc tcaccgccaa
     2101 cggcaaggtc gaccacggcg ccctgcccga tccgaacgcg gtgctcgccg gccaccgcga
     2161 gccggagacg gagctcgagc gggctctcgc cggcctcttc cgcgacatcc tgaaggccgg
     2221 acgcgtcggg atcgacgacg acttcttcgc cctgggaggc agctcgatca ccggcgccat
     2281 gctgatcaat cgcctgcagc gggagctggg ggagatcgtg caggtggtgg tgatcttcga
     2341 tcaccccacc gtcggacggt tggccgccta cctccgcgag cagcaccccg agggggtgac
     2401 ccggtggctc ccccaggaga ccccggccgt gccggtcgcc gccgagagcg ccgccggccg
     2461 ggtggacgag gggatggtgg aacggttgcg gcagctcgtc ccatccttcc ccgcgtcgtc
     2521 ggcttcgttc tccctcgccg cgcggggagc gaagaaccct cccgcgctgt tcgtgctggc
     2581 gcctccccgc tcgggcacca ccctgttgcg ggtgatgctc ggcgggcatc cgcggctgtt
     2641 cgcgcccccc gagctcgagt tgctcgggtt ccccacgatg gcggaccgcc gcgcggcctt
     2701 cgccggccgc gacagcttct ggctcgaagg tctgctgcgg gcgctcaagg agatgcgcgg
     2761 cggttcgccc gaggaggtgc gcgcctgggc cgaggagcac gagcggcggg ggacgccgac
     2821 gctggatctc tacgcgctgc tgcaagagga gctggcgggg cgctggctgg tcgacaagac
     2881 cccgtcctac gccctcgatc ccgcggtgct gcgccgcgcc gaggagggct tcacggcggc
     2941 gcgctacctc cacctggtcc gccacccctg cggcatgatc cgctcgttcg aggaggccag
     3001 gctcgatcag atcttcttcc gccgcgcgca cccgttcacc cgccggcagt tggcggagct
     3061 gatctgggtg gtctcgcacc agaacattct gagcttcctg gccgatgttc cggccgaccg
     3121 ccagcacacg gtgcgcttcg aagagctgct cgccgcgccc gagcgggtcc tggggggggt
     3181 ctgcgatttc ctcggcctgg agtatcaccc ggacatggcc cgcccctacg aggggggatc
     3241 ggcgcggatg accgacggcc tccatgccgc gtcgcggatg ctgggagacg tcaagttcca
     3301 ccagcactcc ggagtggacg cggaagtggc ggagaggtgg cgggacggcc tccgcgaagc
     3361 ggatctcggc tcgccggcgc ggcgggtcgc cgccgcgctc gggtatccgc cgggcccgcg
     3421 cgggctcgac cgaggggcac gcatcgagcg gggcacctgg ggaccgggcg agccgctgcc
     3481 actgtcgttc gcccaggaga ggctctggtt cctcgaccag ctccatcccg gcacggccac
     3541 ctacaacatc cctgtggccc tgcgcctgct gggccgtctc gacgtggccg gcttgcgggc
     3601 cgccttgacg gaagttgcgc gccggcactc ggtcctgcgc accaccttcg cgtccgcgga
     3661 cgggcggccg gtccaggtgg tgcaggcggc gcaacccgtt ccgctgccgc tcgtggacct
     3721 ggccggtttg ccgccggatg aacgggagag ggaggcgctc cgtgtggcgg gggaggcgag
     3781 ccggcggccg ttcgacctcg cccgcggtcc ggtgctgcgg gccgggctgt tgcgcctcgg
     3841 cgaggcagag cacgtggcgc tggtcggcat gcaccacatc gctagcgacg gctggtctgt
     3901 aggcgtgttg atccgcgagc tgggggcgct ctggccggcg ctgggccggt cgggctcgcc
     3961 gccactgccc gagcccgcca ttcaatatgc cgacttcgcg ctctggcaga gagcctggct
     4021 caccccggag agactggcgg cgaacctcgg ggactggaga gaacggctgg cggacctgcc
     4081 gcccctcgat ctgctgccgg accggccgcg cccacccttc gccagcggtc ggggcgcgac
     4141 cctgtcgttc ccgctgccgc cgcatctggg cgaggcgctg gcggtcgccg gcctgcggac
     4201 cggcgcgacg ctcttcatgg ccgctctcgc ggggttcatg gcgctgctga gccgctacgc
     4261 ggggcaggac gacttcgcgg tggggacccc tgtcgcgggg cgccaccatg ccgagctcga
     4321 ggagctgatc gggttcttcg tcaataccct ggtattgcgc gccggcctcg agggctcccc
     4381 cgggttcgac gagctcgtcg gccgggtgcg gcagaccgtc ctgtgggcct tcggccatca
     4441 ggacgtgccc ttcgagcgtc tggtcgagga gctccagccg cagcgggacc tcgcccgtcc
     4501 gcccctgttc caggtgatgt tctccctgca gaacgcctca tccgagaggt tggcgctgcc
     4561 ggggctcagc ctgcactctt tgcagcaggc cgtgacgacg gcgaagttcg agctgaccct
     4621 gtctctcgcc gagaccgtgg cgggcctcgc cggggagatc gaatacgcca ccgatctgtt
     4681 cgatcgcacg acgatctcac ggctggcgga gcacttcgcg gccctgctgt cggcggccgt
     4741 agccgcgccg tcgatccgcc tgacggacct gccgctgctc ggcgcggccg agcgccagca
     4801 gctcctgctg gagtggaacg acacgcaggt gacccggcgg ggagccgccg tgcgcatcca
     4861 cgagctgttc gaggagcagg cggcgcggcg tccccaggct ccggccgtat ccgggcaggg
     4921 gacgacgctc tcctatgccg agctcgaggc gcgcgccaac cggctggcgc gccgcctgcg
     4981 gcgcctcggc gtggggccgg agacacgcgt cgctctttgc gtcgacagat cgccggagat
     5041 ggtggtggcg ctcctcggca tcctcaaggc cggcggagcg tatgtgccgc tcgatccggc
     5101 gcacccggcc gagaggctgg cgctggtgct cgatgacagc gcgccgcagg cgctggtcac
     5161 ggaggagcgc tggctggacc ggctggcgtt cgacggtccg cggatcctct gcctggaccg
     5221 cgaccgcgag tcgatccacg ccgaggaggc cgccgctctg ggagcgccaa ccggcggcgg
     5281 agcggagagc ctggcctacg tcatctacac ctcggggtcg accggccggc cgaagggtgt
     5341 ctgcctgccg cacggcgcgg tggtgaattt cctgttcgcc atggtggagc ggctgggcct
     5401 cggggcggac gacgtggtgc cggcgctgac gacgttgacg ttcgacatcg ccgggctgga
     5461 gatctatctg cccctggcgc tcggcggccg ggtggaggtg atcggtggcg aggagggctc
     5521 cgacggccac cggctggcgg cgcgcctggc cgcggccgga gtcacggcca tgcaggccac
     5581 gcccgcgacc tggcgtctgc tgctcgatgc cggatggagc ggacgggagg gcttcaaggc
     5641 cctctgcggc ggtgaggcgc tgccgcggag cctggcggcg gagctgctgg cacgcggtgc
     5701 cgggctgtgg aacctgtacg gtccgacgga gacggcgatc tggtcggcca cgggatcggt
     5761 ggcgccggcg ggggaggccg tcgccgtggg cctggggcgt tccattgcca atacgcggct
     5821 ccatgtcgtg gaccgccggt tcgagccggt gccgccgggg gtgaccggcg agctgctgat
     5881 tgccggcgcg ggaatggcgc gcggctactg ggaccggccg gatctgactg cggagcggtt
     5941 cctgccagac ccgtggagca agcccggcgg ggcgcggctc taccggaccg gtgacctcgt
     6001 gcgccatcgc gctgacgggg agctggagtt cctcggccgc gtcgaccacc agatcaagct
     6061 gcgcggcttc cgcatcgagc tgggggagat cgaggtcgcg ctgctgcggc actcccaggt
     6121 gagtcaggcc gcggtcgtgg tggtgggcga ggggaccgac cggcggctgg tggcctgt
//
LOCUS       DASPJI010000123         5313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_178821, whole genome shotgun sequence.
ACCESSION   DASPJI010000123 DASPJI010000000
VERSION     DASPJI010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178821"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VEL74_03455"
     CDS             <1..416
                     /locus_tag="VEL74_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61613.1"
                     /translation="DDGCAIKEVLRGVPTRMSLTFPNFGVESSLVQRLRIHWGENPYC
                     HEERSVDFEKIAFSDDGVSSSKRAGNGNSGDGGGGAAKGKGGLLQRFGDQIMNRLEGA
                     ATEMLDKEINKLTGDDDEEEEAEEEDAKPKKKKKE"
     gene            444..2234
                     /locus_tag="VEL74_03460"
     CDS             444..2234
                     /locus_tag="VEL74_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYG61614.1"
                     /translation="MDYQDFVLQLDRAPGGDGFITRVIRSPAGEAEAPFVNPVRPQDL
                     DFLWEGAEDARKAEGIGNTRDAVSMASSTARDQVEAERSLEELGERLFLALFHGPVRS
                     CWARSLEQSVHDPEKGLRLKLQVNLEDRALAPLMELPWEYLFSPEHRGFLGLQRQTPI
                     LRHVRLPLPPGQPPVAQALRVLIVSLQPERMRRLSLARESERIASVLAKLPGVQALPL
                     HNPTVETLRDTLLRQQFHVLHFMGHGGFDTASGQGVLYFAGEGGASVPVGGRLLASHL
                     TGLPSLRLVFLNACDTGRSNASAPFAGVATALLRAGLPAVIAMQQPIRDGAALELSRA
                     VYRRLAAGDPIDAAVTEGRLAIARGHGALLEWGTPVLFLRSDDGRLFEAAAKPSAPPA
                     EPLAPLFPAAPPAPARQGSRRLLSSLLIAAVLAGAAGIGASRWLASPAEESRGPARQQ
                     EAVVEPSPTPEPAPTPEKEEKRLVRGDVVQPTRTVPRDEPPAPRVEKEEAPPAPPPSS
                     PGSYVVSDGGSVSIPVLEVDVGASFHGREGYPFARFWIAPWGGEMLQQPPIMGPKSIA
                     FPAPDGTYHLDVLTLDFEGKRATVRVRFAS"
     gene            2242..2625
                     /locus_tag="VEL74_03465"
     CDS             2242..2625
                     /locus_tag="VEL74_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYG61615.1"
                     /translation="MLPRTMDVTTIPILPCRDLDETLSFYGALGFVLEFEQTEPDVYA
                     ILQLEGAEIHFFGHPGVDPWTSHAGCYVRVSDADAIYARWSQLGLPTKGIPRLDAIAD
                     KPWGMRELAVVDPSGNLIRVGQILE"
     gene            2721..3305
                     /locus_tag="VEL74_03470"
     CDS             2721..3305
                     /locus_tag="VEL74_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61616.1"
                     /translation="MRFQGMDRNGAGRITRDEWRGNDQSFSRHDWNGDGVLSGEEVRP
                     GGRRGGDDDDREPGRFDRLDTNDDGYISRAEWPFDDQLFDRLDQNNDNRVSRGELDVL
                     GREGDWEDRFDLMDENGDDRVSRGEFIGRTGLFDRWTAMTTISSAPRSSGKASRGAAV
                     ASAVRTGPSGASTATATAILPSRSGSAGTMTDPG"
     gene            3497..4549
                     /gene="nadA"
                     /locus_tag="VEL74_03475"
     CDS             3497..4549
                     /gene="nadA"
                     /locus_tag="VEL74_03475"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020041086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HYG61617.1"
                     /translation="MSILEASRHRAALDFTPEVARATAPIRERVRSVIPDVEWPLHAP
                     YIAAINELKQELGAAILVHNYQTPEIFHGVADFTGDSLALAQYGARTDAEVIVLAGVH
                     FMAETAKILSPEKTVLIPDLYAGCSLASSITAEDIRLLRQRYPGVPVVTYVNTSAAVK
                     AESDICCTSANAVAVVESLGVDRVIFLPDEFLGKYVAQQTDVEVILWKGHCEVHERFT
                     GAELRAYREAHGGDIHILAHPECPPDVLAEADFVGSTAAMISHVGERRPKRVVMVTEC
                     SMSDNVAVEFPDVEFVRPCSLCPHMKRISLPGILRSLQRMEHQVEVGPEVAGRARLAL
                     ERMLAVGRKEMKGAAR"
     gene            4546..>5313
                     /locus_tag="VEL74_03480"
     CDS             4546..>5313
                     /locus_tag="VEL74_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016472901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYG61618.1"
                     /translation="MTQASRGFDGVVSSDVLVVGGGVAGLTAALGCVPRRVTVLTKAP
                     LGGGGASVWAQGGVAVAVGRDDSPALHSADTLSAGAGLNDAAVVDLLTGEGPRRVRAL
                     LEMGARFDRDAAGGLALGREAAHSRRRILHAQGDATGAEIVRTLMEAVRRTPEIRVEE
                     GAFALDLVTDPASGEVVGLVAVHADGRRLLHLAPAVVLATGGLGQLYLHTTNPREATG
                     DGIAMAARAGARLVDLEFVQFHPTALAVPPADDSEEGS"
BASE COUNT          799 a         1838 c         1857 g          819 t
ORIGIN      
        1 cggatgatgg ctgcgctatc aaggaggtgc tgagaggtgt gcctacgcgc atgtcactga
       61 catttcccaa cttcggggtg gaatcgtcgc tcgttcaacg actccgtata cattggggtg
      121 agaatccgta ttgccatgaa gaacggtctg tggacttcga aaagatcgcc ttctcagacg
      181 acggcgtctc ttcctcgaag agagcaggca atggcaatag tggcgacggc ggcggaggag
      241 ctgcaaaggg caagggcggc ctgctgcagc ggttcgggga tcaaatcatg aacaggctcg
      301 agggcgccgc cacggagatg ctcgacaagg agatcaacaa gcttaccggc gatgacgatg
      361 aggaagagga ggcggaagaa gaagacgcca agcccaagaa aaagaagaag gagtagccgc
      421 tcggcactcg aggacgaacg gccatggact accaggactt cgtgctgcaa ctcgatcgcg
      481 ctcccggtgg ggacggtttc atcactcgcg tgatccgttc gcccgcgggc gaggccgagg
      541 ccccgttcgt caatccggtc cggccgcagg atctcgattt cctctgggag ggggcggagg
      601 atgcccgcaa ggcggagggg attggcaaca cgagagacgc ggtgtccatg gcctcctcga
      661 cggcgcgaga ccaggtggag gcggagcgct cgctcgaaga gttgggagag cgcctctttc
      721 tggccctgtt ccatgggccg gtgcggagct gctgggcgcg cagcctggag cagtcggtgc
      781 acgaccccga aaaggggctg cggctcaagc tgcaggtgaa cctggaagac cgggccctcg
      841 cccccctgat ggagctgcct tgggagtacc tgttctctcc cgagcatcgt gggtttctgg
      901 ggctccaacg ccaaacaccc atccttcgtc acgtgcggct gcctctacct ccggggcagc
      961 cgccggtggc gcaggccctc cgagtgctca tcgtgtcctt acagccggaa cggatgcgcc
     1021 ggttgagcct ggcccgcgag agcgagcgga tcgcctccgt cctcgcgaag cttcccggag
     1081 tccaggcgct gccgctgcac aaccctacgg tcgagaccct gcgggatacc ctgctgcgcc
     1141 agcagttcca cgtcctccat ttcatgggcc acggaggctt cgacaccgcg agcgggcagg
     1201 gagtcctcta cttcgccggg gagggcggtg cctcggtgcc cgtgggcggg aggctcctgg
     1261 cctcacacct caccggcctg ccatccctcc gcctggtctt cctgaatgcc tgcgatacgg
     1321 ggcgctccaa cgccagcgcg ccgttcgccg gagtggccac ggccctgctg cgggccggcc
     1381 tgccggcggt catcgcgatg cagcagccga tccgcgacgg cgcggccctg gagctcagcc
     1441 gcgccgtgta ccggcgcctc gccgcaggcg acccgatcga cgccgcggtc accgaagggc
     1501 gcctcgccat cgcccggggc catggcgcgc tgctcgagtg gggcacgccc gtccttttcc
     1561 tgcgctcgga cgacggccgg ctgttcgagg ccgccgccaa gcccagcgcg ccgcccgccg
     1621 agccgctcgc tcccttgttc ccggccgcgc cccccgctcc agcgcgccag gggtcccgga
     1681 ggctcctctc ctcgttgctg atcgccgccg tgctggccgg agccgccggc atcggggcgt
     1741 cgcgctggct ggccagcccc gcggaggaga gcagggggcc ggcccggcag caggaggcgg
     1801 tcgtggagcc gagccccacc cctgagccgg ctcccacgcc cgagaaagag gagaagaggc
     1861 tggtcagagg ggacgtcgtc cagccaacgc gaacggttcc ccgggatgag ccgccggcgc
     1921 cccgggtaga gaaggaggag gcgcctcccg cgccgccccc gagctctccc ggctcctacg
     1981 tggtcagcga cggcggctcg gtctccatac ccgtcctgga ggtcgacgtc ggggcttcgt
     2041 tccatggacg ggagggctac ccctttgccc gcttctggat cgcgccctgg ggaggcgaga
     2101 tgctccagca gccgccgatc atggggccga agtcgatcgc gttcccggcg ccggacggca
     2161 cctatcacct cgacgtcctc accctcgact tcgaggggaa gcgggccacc gtgcgggtgc
     2221 gcttcgcctc ctgacccctg tatgctcccc cgcaccatgg acgtcaccac catccccatc
     2281 ctcccctgcc gtgacctcga cgagacgctg tcgttctatg gcgccctcgg gttcgtcctc
     2341 gagttcgagc agaccgagcc cgatgtctac gccatcctgc agctcgaggg ggccgaaatc
     2401 cacttcttcg gccatcccgg cgtcgacccc tggacctccc acgccggctg ctatgtccgg
     2461 gtctccgacg ccgacgcgat ctatgcccga tggtcccaac tgggcctgcc gacgaagggc
     2521 atcccgcggc tcgacgccat cgcggacaag ccgtggggga tgcgcgagct cgctgtcgtc
     2581 gatcccagcg gcaacctgat ccgcgtcggc cagattctgg agtgatcgcg ttggcacctc
     2641 ccttgtgatg aatgcgcaca gacgtatcgg gacatggagg cgcagctctc cgtgtcctct
     2701 ggagcgcaaa cgacggcacc atgcggtttc aaggcatgga ccgcaatggc gccggccgga
     2761 tcacgcgcga cgagtggcgc ggcaacgatc agtcgttcag ccgccatgac tggaacggcg
     2821 acggcgtcct ctccggcgag gaggtccgcc ccggcggccg ccgcggcggc gatgacgacg
     2881 accgcgagcc gggccgcttc gaccgcctcg acaccaacga cgacggctac atttcccgcg
     2941 cggagtggcc gttcgacgac cagctcttcg atcgcctcga ccagaacaac gacaaccggg
     3001 tgagccgggg tgagctcgac gtcctcgggc gtgagggtga ctgggaggac cgcttcgacc
     3061 tgatggacga gaacggcgac gaccgcgtga gccgtggcga gttcattggc cggacgggtc
     3121 ttttcgaccg ctggaccgca atgacgacaa tttcctcagc gccgaggagt tccgggaagg
     3181 cgtcgagggg agcggcggtg gcgtccgcgg tgaggaccgg accttccggc gcctcgaccg
     3241 caacggcgac ggctatctta ccttcgagga gtggcagcgc cgggacgatg actgatccgg
     3301 gctgacttcc cgctgaatcc gggcccccgc tcccgtcccg acgggggtgg ggctttcctc
     3361 tctccatccc ctcgtcacgg ccccgtcacg gggaattgac acccctaatg ctcagttata
     3421 ggataaggaa ggccggcgtg ggccggccct ttcttatggc tactatatgc tcaagatgag
     3481 cataggggga gatcggatga gcatcctgga ggcttcgcgt caccgtgccg cgctggactt
     3541 cactccggag gtggcccgcg ccaccgcccc gatccgggag cgggtgcgtt cggtcatccc
     3601 cgacgtcgag tggccgctgc acgcccccta cattgccgcg atcaacgagc tgaagcagga
     3661 gctcggggcg gccatcctgg tccacaacta tcagacgccg gagatcttcc acggcgtggc
     3721 cgacttcacc ggcgactccc tggccctggc ccagtacggc gccaggacgg acgccgaggt
     3781 catcgtgctg gccggcgtcc actttatggc cgagacggcg aagatcctgt cgccggagaa
     3841 gacggtgctg atcccggacc tctatgccgg ctgctcgctg gcgtcgtcga tcacggccga
     3901 ggacatccgg ctgctgcggc agcgctaccc gggcgtgccg gtggtgacct acgtcaacac
     3961 ctcggccgcg gtgaaggccg agtcggacat ctgctgcacg tcggcgaacg ccgtcgcggt
     4021 ggtggagtcg ctgggcgtcg accgcgtcat cttcctgccc gacgagttcc tgggcaagta
     4081 tgtggcgcag cagaccgacg tcgaggtcat cctctggaaa gggcactgcg aggttcacga
     4141 gcgcttcacc ggcgccgagc tgcgggcgta ccgcgaggcg cacggcggcg acatccacat
     4201 cctggcgcac cccgagtgcc cgccggacgt gctggccgag gccgacttcg tgggctcgac
     4261 ggcggcgatg atctcgcacg tcggcgagcg ccggccgaag cgggtggtca tggtgacgga
     4321 gtgctcgatg agcgacaacg tggccgtgga gttcccggat gtcgagttcg tccgcccctg
     4381 ctcgctctgc ccgcacatga agcggatctc gctgccgggc atcctgcgct ccctccagcg
     4441 gatggagcac caggtggagg tcggccccga ggtggccggc cgggcccgcc tggccctcga
     4501 gcggatgctc gccgtcggcc gcaaggagat gaagggagcg gcgcgatgac ccaggcctcc
     4561 cgtggcttcg acggcgtggt gtcgtccgac gtcctggtgg tgggcggcgg cgtggcggga
     4621 ttgacggcgg cgctcggctg cgtgccccgg cgggtgacgg tcctcaccaa ggcccccctc
     4681 ggcggggggg gcgccagcgt ttgggcccag ggcggcgttg ccgtcgccgt gggccgggac
     4741 gactcgccag ccctgcactc cgccgacacc ctgtcggccg gcgccgggct caacgacgcc
     4801 gcggtcgtgg accttctgac cggcgagggg ccgcggcgcg tccgcgccct gctggagatg
     4861 ggggcccgct tcgaccgcga cgccgcgggc ggcctggccc tcggccgcga ggccgcccac
     4921 agccggcggc gcatccttca cgcccagggc gacgccaccg gcgccgagat cgtgcgcacc
     4981 ctcatggagg cggtgcgccg cacgcccgag atccgggtgg aggagggggc cttcgccctc
     5041 gatctggtca ccgatcccgc gagcggcgag gtggtcgggc tggtggcggt gcacgccgac
     5101 ggccgccggc tgctccatct ggcgcccgcc gtggtgctgg ccaccggcgg cctggggcag
     5161 ctctatctcc acaccaccaa tccccgcgag gccaccggcg acggcatcgc catggcggcg
     5221 cgggccggcg cccgcctggt ggacctggag ttcgtacagt tccatcccac ggcgctggcg
     5281 gttccccccg cggacgattc ggaggagggg agc
//
LOCUS       DASPJI010000124         3333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_579923, whole genome shotgun sequence.
ACCESSION   DASPJI010000124 DASPJI010000000
VERSION     DASPJI010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579923"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VEL74_03485"
     CDS             <1..194
                     /locus_tag="VEL74_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61619.1"
                     /translation="VVGELQLFDEAAVDAMNVAVHLLQSPESLAYLLEAAGAVALERC
                     GAILDERVGDGTTPVQAGE"
     gene            577..1119
                     /locus_tag="VEL74_03490"
     CDS             577..1119
                     /locus_tag="VEL74_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HYG61620.1"
                     /translation="MALTATIYNFDISLSDVDRGVYEALAVRVALHPSETMEYLLSRL
                     FAYCLEYEEGITFSKGGISDGDQPPLWVATPDGRMKTWIEVGMPDPERLNKAGKGAER
                     VVVYTHRDPAQIRRQAEGERIHRAGEVRIFALDKQLLAGLAPLIDRRTKMEISVTGGH
                     LYVNVAGRSLSGEVTEHRLE"
     gene            complement(1154..1747)
                     /locus_tag="VEL74_03495"
     CDS             complement(1154..1747)
                     /locus_tag="VEL74_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HYG61621.1"
                     /translation="MESHDNTQGITQLLAAVRAGDNPASDALFDRVYQDLHRLAHAQL
                     AARGRAGDTLGTTVLAHEAYLRLAQPGSLSAGDRAHFFNLAARVMRHVVVDYARRRDA
                     AKRSGDVVSLDATGAQEPEAESSYALQEELLALDAALQRLEATSPRLARLVELRFFAG
                     LGFAEIGEAQGMTERTAKRDWRKARAFLLAELSGLPA"
     gene            complement(1766..>3333)
                     /locus_tag="VEL74_03500"
     CDS             complement(1766..>3333)
                     /locus_tag="VEL74_03500"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG61622.1"
                     /translation="AEQVKELLVSIFRQADPETGQGPDLTAGEILAAGAKRVETELAR
                     EPRIQAELLDAIAQIQGSLGQFDPAAEAASTALARRRALFGAESVPVAQSLTTLAELA
                     ANRGRLEEAIRLAERAGPAFERHAGPESPDRQRLDRVRVFLLRHQAEPERALALVEEL
                     LEANRRHFGPTSVQTARLLINRAEVLGDLSRFAEAEQAVRQALAILEASPQVTRLEVS
                     YARRGLADLLESLDRHQEAAAEFDAVLALQRAELGPRHRQVAFTRIAQGFLLSEMRRF
                     PEAEATLREAIRILEPLDHFEIGSARCYLGICMMSQERYAEAERQFSEAERFLRTKLE
                     ADHPLVWGSRISQGIAQLRLGRLGEARRTLSEVVDHYARTEPESDGNRSALKHLGEVA
                     WREGRIDEALALHRQARQIEVKIFGTEKHVGVAFSDYLIALDLLARGDPGSLAEARQR
                     IDAAIAVLRHENAGHPTLNRFLLASGRIALAVGDEARARQDLTDAVARLRGFRGPDHP
                     DTREAEALLRRAS"
BASE COUNT          470 a         1171 c         1208 g          484 t
ORIGIN      
        1 aggtcgtcgg cgagctgcag ctcttcgacg aagcggccgt ggacgccatg aacgtggccg
       61 tccaccttct tcagtcaccc gagtcgctgg cgtatcttct ggaggcggcg ggagctgtag
      121 ccctcgaacg ttgcggggcg atcctcgacg agcgggtcgg cgacggtacg accccggtgc
      181 aggccgggga gtagggcttc cgcatcgaga tggagagagt cgccgccgtc aggacccgtc
      241 ctggcggcgg tttgacccgc ggggcaagca acacgaccta ccaatgacac cgctcgacct
      301 gtgatcaaca cctcttgccc ttggggtcga acgtctcgcc cccgggggca gatcgcttgc
      361 cccgtgggtc aagtacgtcg ccccagaggt cagaccgctt gccccgtggg gcaggcacat
      421 caccccagag gtcagaccgc tcgccccgtg ggtcaggcac gtcaccccag gggtcagacc
      481 gctcgcccct cagggcagat ggctcgaccc ttgggtcaaa ccgcttgacc ctggcgagaa
      541 tccccgccga gcagcgccgt agtagactcc cgtcccgtgg ccctgaccgc gaccatctac
      601 aacttcgaca tctccctgtc cgacgtggac cgcggcgtct acgaggcgct ggcggtgcgc
      661 gtggccctcc atccctccga gacgatggag tacctgctga gccggctgtt cgcctactgc
      721 ctggagtacg aggaggggat caccttctcc aaggggggca tctcggacgg ggaccagccg
      781 ccgctctggg tcgcgacccc cgacggccga atgaagacct ggatcgaggt cggcatgcca
      841 gaccccgagc ggttgaacaa ggcggggaag ggggcggagc gggtcgtcgt ctacactcac
      901 cgggacccgg cccagatccg ccgccaagcg gaaggggagc gcatccaccg cgcgggcgag
      961 gtccgcatct tcgctctcga caagcagctc ctcgccggcc tcgcccctct catcgaccgc
     1021 cgcacgaaga tggagatctc cgtcaccggc ggccacctct acgtcaacgt ggccggccgc
     1081 agcctctcgg gcgaggtgac tgagcaccgg ctggagtaga aacgaaaccg cccgacatcc
     1141 ctcaccggga gttctaggca ggaaggccgg acagctccgc caggaggaag gcgcgggcct
     1201 tgcgccagtc gcgcttggcc gtcctctcgg tcatgccctg ggcctcgccg atctcggcga
     1261 agcccagccc ggcgaagaag cggagctcga ccaggcgggc gaggcggggg ctcgtcgctt
     1321 ccaggcgctg gagggcggcg tcgagggcga gcagctcttc ctgcagcgcg tagctcgact
     1381 cggcctctgg ctcctgggcc ccggtcgcgt ccagggagac cacgtcgccc gaacgtttgg
     1441 cggcgtcccg gcgccgggcg tagtccacca ccacgtgccg catgacccgg gcggcgagat
     1501 tgaagaagtg ggcgcggtcg ccggcgctga ggctccccgg ctgggccagg cgcagatagg
     1561 cctcgtgcgc caggacggtg gtccccaggg tgtcgccggc ccggccgcgg gcggcgagct
     1621 gggcgtgggc gaggcgatgg aggtcctgat agacgcggtc gaacagggcg tcggaagcgg
     1681 ggttgtctcc ggcccggacc gccgccagca gttgggtgat cccctgggtg ttgtcgtggg
     1741 attccatgga gaaggttctc tctatctatg aggcgcggcg gaggagggcc tccgcctcgc
     1801 gggtatccgg atggtcgggg ccgcggaagc cgcgcaggcg ggccacggcg tcggtgaggt
     1861 cctgccgcgc ccgcgcctca tccccgacag cgagggcgat gcggccgctg gccagcagga
     1921 accggttgag cgtgggatgg ccggcgtttt cgtggcgcag cacggcgatc gccgcgtcga
     1981 tccgctggcg cgcctcggcc aggctcccgg gatcgccccg cgccagcagg tcgagggcaa
     2041 tgaggtagtc cgaaaaggcc acccccacgt gcttctcggt accgaagatc ttcacctcga
     2101 tctgccgcgc ctgccggtgc agggccagcg cctcgtcgat ccgcccctcc cgccacgcca
     2161 cctcgcccag gtgcttgagg gcgctgcggt tgccgtcgct ctccggctcc gtgcgcgcgt
     2221 agtggtccac gacttcggac agcgtccgcc gggcctcgcc gaggcggccg agccggagct
     2281 gggcgatccc ctggctgatg cgcgagcccc acaccagcgg gtgatcggcc tccagcttgg
     2341 tccgcaggaa gcgctccgcc tcgctgaact gtcgctccgc ctcggcgtag cgctcctggc
     2401 tcatcatgca gatgcccagg tagcaccgcg ccgagccgat ctcgaagtgg tcgagcggct
     2461 cgaggatgcg gatcgcctcg cgcagcgtcg cctcggcctc ggggaagcgc cgcatctcgc
     2521 tgagcaggaa tccctgggcg atccgggtga aggccacctg gcggtgccgc ggccccaact
     2581 ccgcgcgttg cagggcgagg acggcgtcga actctgccgc ggcctcctga tgccggtcga
     2641 ggctctcgag caggtcggcg aggccacggc gggcatacga cacttcgagc cgggtcacct
     2701 ggggggacgc ttcgaggatg gcgagggcct ggcggacggc ctgctcggcc tcggcgaagc
     2761 gcgacaggtc gcccagcact tcggcgcggt tgatgagcag ccgcgcggtc tggacgctcg
     2821 tgggaccgaa gtgccggcgg ttggcctcca gcagctcctc gacgagggcg agggcccgct
     2881 caggctccgc ctggtgccgg agcaggaaga cgcggacacg gtcgaggcgc tgccggtccg
     2941 ggctttccgg gccggcatgc cgctcgaagg cgggccctgc ccgctcggcc agccggatcg
     3001 cctcttccag acggccacgg ttggcggcca gctcggccag ggtggtgagg ctctgggcca
     3061 ccggcaccga ctcggcgccg aacagcgcgc gccgccgggc gagggcggtg gacgccgcct
     3121 ccgccgccgg gtcgaactgg ccgaggctgc cctggatctg ggcgatggcg tcgagcagct
     3181 cggcctggat ccgcggctcc cgggcgagct cggtctccac ccgcttggcg cccgcggcga
     3241 ggatctcgcc ggcggtcagg tccgggccct ggccggtctc cgggtcggcc tgcctgaaga
     3301 tggacaccag cagctccttg acctgctcgg ccc
//
LOCUS       DASPJI010000125         5574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_7519, whole genome shotgun sequence.
ACCESSION   DASPJI010000125 DASPJI010000000
VERSION     DASPJI010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7519"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VEL74_03505"
     CDS             complement(<1..419)
                     /locus_tag="VEL74_03505"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HYG61623.1"
                     /translation="MTVESTFQYRGDLAQTALPEILSTIDRFQVPGVIEASREGVVKR
                     VFLKEGNVVHASSTDRDDSLGSYLERNGVLTAEVVAETMRERQRSNKRYGVLLIERGI
                     LSPGEVYRAIRKQIEAIVWSLFYWQDGGVSFSIGDFR"
     gene            536..1726
                     /gene="glp"
                     /locus_tag="VEL74_03510"
     CDS             536..1726
                     /gene="glp"
                     /locus_tag="VEL74_03510"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HYG61624.1"
                     /translation="MLHPDEAWRRIAEHLTPLPEERLARRAAAGRVLARPLAATVDVP
                     AADVSAMDGYALAGPVPPGEPRPVSGTVVAGDPPGFELRPPSVARIMTGAPVPRGADR
                     VIPVELSDGGREEVVFRGEAAAGEHIRRRGEILRAGDPLSPAGSLLTPGALALLATHG
                     YGEVPVHRAPAVAVLATGDEVVPPDSEPGPGQLRDSHTDFLLAAGASLGLRFTSLGIA
                     PDRVDELRERVRTGLAGDVLLLCGGVSMGEFDLVEGVLADLGCRAVFDAVAIQPGKPL
                     VFATHPGGLVFGLPGNPASVMVAFWLFVRPALRRLLGHDDAWWQGALTGVLAAPLPGS
                     GPRDRFLPAEITTRDGALHVTPAPPQGSHDLAAYARGTGLVRVRAGAQPAPAGAPCEV
                     LRLV"
     gene            complement(1982..2326)
                     /locus_tag="VEL74_03515"
     CDS             complement(1982..2326)
                     /locus_tag="VEL74_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61625.1"
                     /translation="MKRTALFGLAALLLMATLAGAMPAETDENVATLFAPETPECGDA
                     ASAPEPYLAQATDFTCGLCSAPACQNSSPTTYCGITATGVKKYCQATTACTGEGILVR
                     RCLCVVGGDIIP"
     gene            complement(2954..3053)
                     /locus_tag="VEL74_03520"
     tRNA            complement(2954..3053)
                     /locus_tag="VEL74_03520"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3017..3019),aa:Sec,seq:tca)
     gene            3322..3792
                     /locus_tag="VEL74_03525"
     CDS             3322..3792
                     /locus_tag="VEL74_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61626.1"
                     /translation="MGSGGGSQDAGFDFDLPAGWQSEPPANNMRLAQASVPGEGGAGQ
                     LAVFFFGPGGGGGVEDNLQRWIGQMEVDPGTQPERGTLEANGFRITWVDVKGTLLPST
                     MGMGPTTEQPGSRLLGAVVEGEGGPWFFKLTGPDATLSAQREAFFTMLRSVRKA"
     gene            complement(3818..>5574)
                     /locus_tag="VEL74_03530"
     CDS             complement(3818..>5574)
                     /locus_tag="VEL74_03530"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HYG61627.1"
                     /translation="SYLRLHQRLRHRPEVIGGTLFEGLARLAERAGVRHPVRLGCSSR
                     LPVPVALGVRRPQICVPPRALAQLTAEQQEGMLAHELAHVVRRDPFWLALSNAMVSVF
                     FFQPLNWVARRRMREISELLCDEWAVARTGRPLSLARCLAEVAGWSFEPVRPPLPAAG
                     MADRPSHLARRIRRLLEESGSPEKASRRRWIAVGIGVLLLAVAAVAPGVSAAVGAPAA
                     LAAIEAPAPPETPAAEEAPDAGEAPEAPEAWDDEADEDGEDGHEHLSSLHGTLSEIDE
                     QMADLDLDLDSDLEDLADLDIDLDIDLGELEDLEDLEDLDLSEEELERISRNAEKIAA
                     RVQTAMAPRMERLQELGMRMSGLQGEIPAEEMRKLEAEMEALAEQMRPSDEELERLQA
                     EAERIAASVQDREHPSREERDRIAREARELAERYRPSAEEMRRIQELSQQHRELSRKF
                     MEDHREEIEALRREAQQQSQAARDEIRRQLEQDPELKALRERHQEERRRLLEKRREMS
                     RRDRTAPKAAPAPRPSPRPGVAPQARPAPGAIPQAAPPPPAARPSRPSPPAAPAAPPS
                     PAPAALAQPAPEPPAAGR"
BASE COUNT          809 a         1933 c         1969 g          863 t
ORIGIN      
        1 tcccggaagt cgccgatgct gaagctgacc ccgccgtcct gccagtagaa gaggctccag
       61 acgatggcct cgatctgctt gcggatcgcc cggtagacct cccccggcga caggatgccg
      121 cgctcgatca gcagcacgcc gtagcgcttg ttggagcgct ggcgctcgcg catcgtctcc
      181 gccacgacct ccgccgtcag cacgccgttc ctctcgaggt agctgccgag gctgtcgtcg
      241 cggtccgtcg aggaggcatg gacgacgttg ccttccttca ggaagacacg cttgacgacc
      301 ccctcacggc tcgcctcgat cactcccggg acctggaagc ggtcgatggt ggacaggatc
      361 tccggcagcg cggtctgcgc cagatcccca cggtattgga acgtgctctc gacggtcacc
      421 aaggtgggac ctccctctcc tcgatgcaaa cgacaaccag gatcacgagt gaaaagctgc
      481 cgaagtatac cattccgcta atataggggt ccccaggagg aggtccctgc ccttgatgct
      541 ccatcccgac gaggcctggc gccggatcgc cgagcacctc actcccctgc cggaggagcg
      601 cctggcacgg cgtgcggcgg ccggccgggt gctggcgcga ccgctggcgg cgacggtgga
      661 cgtcccggcg gccgacgtgt cggccatgga cggctacgcg ctggccggcc ccgtgccgcc
      721 gggcgagccg cggccggtgt cgggcacggt ggtggcgggc gatccgcccg gcttcgagct
      781 gcggcctccg tccgtggccc gcatcatgac cggcgcgccg gtgccgcggg gtgcggaccg
      841 ggtgatcccg gtggagctgt cggatggcgg ccgggaggag gtggtcttcc ggggggaggc
      901 cgcggcgggc gagcacatcc gccggcgggg cgagatcctc cgcgccggcg accccttgtc
      961 gccggcgggg tcgctcctca cccccggcgc cctggcgctc ctcgccaccc acggctatgg
     1021 agaggtgccg gtccatcgcg cgccggcggt ggccgtcctc gccaccggcg acgaggtggt
     1081 gccgccggac agcgagccgg ggccgggaca gctccgggac tcgcataccg actttctgct
     1141 cgccgccggc gcctcgctag ggctccgctt cacctccctg gggatcgctc ccgaccgggt
     1201 ggacgagctg cgggagaggg tgcggaccgg cctggccggc gatgtcctcc tcctctgcgg
     1261 cggggtctcc atgggggaat tcgatctcgt ggagggggtg ctggcggacc tcggctgccg
     1321 cgcggtcttc gacgccgtgg cgatccagcc cggcaagccg ctggtcttcg cgacccaccc
     1381 gggcggcctg gtcttcggcc tgccgggcaa ccccgcatcg gtgatggtgg cgttctggct
     1441 cttcgtccgt ccggccctgc ggcggctgct ggggcacgac gacgcctggt ggcagggagc
     1501 cctgaccggc gtgctcgccg cgccgctgcc ggggtcgggc ccccgcgatc gcttcctgcc
     1561 ggccgagatc actacccgag atggcgcgct ccacgtcacc cccgcgccgc cccagggctc
     1621 ccacgacctg gccgcctacg cccgcggcac tggcctggtg agggtccggg caggggccca
     1681 gccggcaccg gcgggagcgc cctgcgaggt gctgcggctg gtttgaccgg agctcgtcgc
     1741 ctccgcacgc cgcggatctc gtccagacgc cgcggacacg atccagacgc tccggacgcc
     1801 atccagacgc ttcgggagct tcccggacgc tccggttcgc atccagtaca accggatgga
     1861 gtccgagagg ctcgggaagc atccgagcgg cttggatggc gtcccattca aacggatgga
     1921 gtccggaagg cccgggaagc ttcccggacg ctccgaaagc cgaacacccg ctgggatcac
     1981 ctcaggggat gatgtctccg ccaactacac agaggcagcg gcggaccaga atcccctctc
     2041 ctgtgcaggc ggtggtcgcc tggcagtact tcttcactcc ggttgctgtg attccgcagt
     2101 aagttgtcgg acttgaattc tggcaggccg gtgcgctgca aaggccgcag gtgaaatcgg
     2161 tggcctgggc aagataaggc tccggagcgc ttgcggcgtc gccgcactca ggtgtttccg
     2221 gggcgaagag ggtggcgacg ttctcgtcgg tctctgccgg catggcgccg gcgagagttg
     2281 ccatcagcag cagcgcagcg agtccaaaca gggcagtccg tttcatcgag gtctcctcat
     2341 agcttgttga cgaaggatcg gcgctacgct gcaccgaacc cttctcgata tgactggaga
     2401 cgtgcggaca tttcagtgca ccctgtagag tgcccggggg aattcgaagc aatcccaggc
     2461 ctacacttac ggaatgaggc ccgccgtcca gttcgtaact cagcagatgg catggacggc
     2521 gggcaaactt gagaagctta gcgaggattt ccggtaatag gagtcccggt tcctatccaa
     2581 ggagggacct cacagtagca aacatcgccg atatactcgt aattcgagca accgtagtag
     2641 cgacagatcc caatgcatgc gtcttcgctc aggacattgg cgtactccgt gtactggcag
     2701 gccgccaacg ccggagtagg tttcattgct agtgctacca ggaatgctcc aagaatcacg
     2761 agtttcaatc taagcgtcat cgcattaccc cctagtacta ccgttacgcc ggtgctcgcc
     2821 tggtctcgcc ggctgagggg agggaggata ctacaggcca agctacaaag gggaacggcc
     2881 ttccaggggt aaggtagtct gaacctgagg ggggaactgg cgtcgagaag ctgtcgtaaa
     2941 tccttgaacc gaatggcgga agcgcatggg aatcgaaccc acccccgacg ccgcaaccgg
     3001 cgccggacaa cggttttgaa gaccgcagga ggcaccagcc cccgagcgct tccgggaggg
     3061 attctagcgg atgtgggtga tactctcccg ctgccacggg ctttccggcc tcgtggagac
     3121 cttcatcaag agagagatag acccatgagt cgcaatccct ggatcgtatc ccttctgttg
     3181 ttgagcctct gtgcgtgcgg cggtgagcag ggagctcctc ccgccggaga ggctggccct
     3241 gatgcagcgg cgggtgccgc atccactccg ccgaggccgg ccgcatcgcc catcggcacc
     3301 ctctcctcca gcgaccgcac ggtgggctcc ggtggcgggt cgcaggacgc cggcttcgac
     3361 ttcgacctgc ccgccggctg gcagtccgag ccgcccgcca acaacatgcg gttggcccag
     3421 gcgtccgtcc ccggagaggg tggcgccggc cagctcgcgg tgttcttctt tggcccgggc
     3481 gggggcggcg gggtggagga caacctgcag cgctggatcg gacagatgga ggtggacccc
     3541 ggcactcagc cggagcgcgg caccctcgag gcgaacggct tccgtatcac ctgggtggat
     3601 gtgaaaggga ccttgctgcc gagcacgatg gggatggggc cgaccacgga gcagcccgga
     3661 tcgcgtctgc tgggagcggt ggtggagggg gagggggggc cctggttctt caagctgacc
     3721 ggccccgatg ccaccctgtc cgcccagcgc gaggccttct tcacgatgct gcggagcgtg
     3781 cgcaaggcct agggcccggc gcacgcccgt ctctcgttca gcggcctgcg gccggcggct
     3841 cgggggcagg ctgcgccaga gccgccggag cgggggaggg cggggcggcc ggagccgccg
     3901 gcggtgacgg ccgggagggc ctcgccgccg gcggcggcgg cgcggcctgc gggatcgccc
     3961 cgggagccgg tcgggcctgg ggagccacgc cgggccgggg cgatggcctc ggcgccggcg
     4021 cggccttggg ggctgtccgg tcgcgccggc tcatctcgcg gcgcttctcc aggagccgcc
     4081 gcctctcttc ctgatggcgc tcgcggaggg ccttgagctc ggggtcctgc tcgagctggc
     4141 ggcggatctc gtcacgggcg gcctgcgact gctgctgggc ctcccgccgg agcgcctcga
     4201 tctcctcccg gtgatcctcc atgaacttgc gggacagctc ccggtgctgc tgggagagct
     4261 cctggatgcg ccgcatctcc tcggcgctgg gccggtatct ctccgccagc tcccgcgcct
     4321 cccgggcgat cctgtcccgc tcttcgcgcg aggggtgctc ccggtcctgg acgctcgcgg
     4381 cgatccgctc ggcctcggcc tgcagccgtt cgagctcctc gtcggacggt cgcatctgct
     4441 ccgccagggc ctccatctcg gcctccagct tcctcatctc ctcggccggg atctcgccct
     4501 gcaggccgga catcctcatg ccgagctcct ggaggcgctc catccgcggt gccatcgcgg
     4561 tctgcacccg ggccgcgatc ttctcggcat tgcgcgagat ccgctccagc tcctcttcgg
     4621 agaggtccag atcctcgaga tcctccaggt cttcgagctc tcccaggtcg atgtcgagat
     4681 cgatatcgag atcggcgaga tcctcgaggt cggagtcgag atcgaggtcg agatccgcca
     4741 tctgctcgtc gatctccgac agcgtcccat gcagggagga gagatgctcg tggccgtcct
     4801 cgccgtcctc gtccgcctcg tcgtcccagg cctcgggagc ctcgggagcc tccccggcat
     4861 ccggggcctc ctcagccgcc ggggtctcgg gcggggcagg cgcctcgatc gcagccaacg
     4921 cggccggggc gccgacggcg gcggagacac cgggagccac ggcggcgacc gcgagcagca
     4981 ggacaccgat gccaaccgcg atccaacgcc gccgggacgc cttctcgggg gacccggact
     5041 cctccagcag gcgccggatg cgccgcgcca ggtgggacgg gcggtccgcc atgccggcgg
     5101 cgggcagcgg cggacggacc ggctcgaagg accagccggc gacctcggcg agacagcggg
     5161 cgagggagag cggccggccg gtccgcgcca ccgcccactc gtcgcacagc agctcggaga
     5221 tctcccgcat ccggcgccgg gccacccagt tgaggggctg gaagaagaag acgctcacca
     5281 tggcgttgct cagggcgagc cagaagggat cgcggcgcac cacgtgggcc agctcgtgcg
     5341 ccagcatccc ctcctgctgc tcggcggtga gctgggcgag ggcccgcggg ggcacgcaga
     5401 tctgagggcg ccgcacgccc agggcgacgg gcaccggcag ccgcgacgag cagcccagcc
     5461 ggaccggatg ccggaccccg gcccgctcgg ccagccgggc gagcccctcg aacagcgtgc
     5521 cgccgatgac ctccgggcgg tgacgcagcc gctggtggag ccgcaggtac gagc
//
LOCUS       DASPJI010000126        12064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_809556, whole genome shotgun sequence.
ACCESSION   DASPJI010000126 DASPJI010000000
VERSION     DASPJI010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12064)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12064
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809556"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1693
                     /locus_tag="VEL74_03535"
     CDS             <1..1693
                     /locus_tag="VEL74_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61628.1"
                     /translation="ILALLILPATAFAAEPAPDPRQILEQVREAYKELSSFREHGTVE
                     VAPAEGSTEPARQRLFELEATPGELTVVVADDQKGGRRDLLWRSGGQFFRYASDGEQL
                     QPVPSLAAGLTGLTSEPEGAAFIVPRLLSEADAAWPGAEPAALEKSERCGAGTCWVIA
                     FAPKDGVVSRLWIDSRTSLIKKSELRGASRTVTVFSEVDEVDGAEVGSRMSWASPGQP
                     DPWKDVTLGPGGDFSEEITVELTTATVRVVDPYGQPVTGLRPEDFLVLVAGREVPVAS
                     VDWVTSKAGPAPADQVAPGASPAPLDESAAAAGPAQPAPAGKLVVFFVQAHMDPSRFR
                     GQIKLRAHTRKLLDTLGAEDRLAVLSFDSHLKLRQDVTRDREAVHRAIDQGMSFGGKP
                     LPRPPAGDPETLAAHLDFERALAAASPEAGLAVAAQALRFLSGEKVIIYLGYGLGKMV
                     ADDVVLRSEYKDALQALGEARASVFVLDITQADFHSLEGSLEQIAYQTGGTYSKTLIF
                     ENLATQTLANAISGHYVLAFDSDTLPRGGGTLRIKLRDGKKGRILVRTPKAQGGR"
     gene            1775..3286
                     /locus_tag="VEL74_03540"
     CDS             1775..3286
                     /locus_tag="VEL74_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61629.1"
                     /translation="MKTTPLLFLLLSLYPLQALPAEPGAGEPTALEILQRVRTTYASL
                     PSFREEGVITSTGGAGRELRFELGAADGKVSLSASGDFDYGEDGMDDEERTALAVPGL
                     LAEAGWPGSRSATLERQEPCGTSNCWVIALTETPEKAEEGGGVLRLWVDTATYYLWRT
                     ELRGPGEFARTLVVEHEPREFAPRAAAGEDLWASAETAVLSKVTLRVTGADGQPVTGL
                     KPQDFRAAVAGQELPVVSVDWVSSQPTGQDGTAPGAGKLVIIFVQAHPQRTRLAGQMQ
                     LRQSTRELLGTLAPEDRVAVVSFDSHLKLRQDFTADREAVHQAIDRGMRFGGETPAPP
                     GNAEPETLAAHLDFGQARAAASPEHALLVTGRALARLPGKKVVLFIGYGLGEHVERDR
                     VAVESQMDEALLLLLQGQTSVFVLDVNQVDRHSMREGLVRLATNTGGTYASTRLSPHL
                     AVRSLAGLVSGHYVLTFDGATIPLEGGTLQVTLRDPKRGKVFIPPLFLAGRPQ"
     gene            3318..4892
                     /locus_tag="VEL74_03545"
     CDS             3318..4892
                     /locus_tag="VEL74_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61630.1"
                     /translation="MAKRGERILAVSSAALTAVFAWMVLSVVPAFAESAQEILQRVRT
                     AYAGLPSFSEAGILTEVTSEGGAEQRHEIRFELAVAGGEVWLTVPLDSEGGKERLGDQ
                     ERAALAVPGLLADATWPGFRQASLEGQEPCGASACWVISLTEETKPGGEILRLWVDTE
                     TSYVWKTELRIPGEAARTLVVEHEPREFGPLTGDDLWATATAQEGQSTLEGFSEEITV
                     GLSTATLWVVDHQGRPVNGLRPEDFRAVLAGREIPVAAVDWVSSRQTGQDGAGAAPAP
                     GKLIVFLVQASPVNVFLSGQMRLRRQIRELLDTLAPEDRVAVLSFDSHLKLWQDFTLD
                     RDAAHRALDRGMLFGGDSPVPPRLAEPGTLAAHFDLEQARAAASVGRALDLTGRALLP
                     LHGEKVVVFLGYGMGESRRDDKEVLAAALASLQQARAPVFVLDIVQGSSHSLVSSLLK
                     LAAHTGGTYAKTYFTPDLATRKLAGLFSGHYVLSFDSGTIQGGGTLQVTLKDSRRGTV
                     LARSVSPVDPDASDGR"
     gene            complement(4910..5884)
                     /locus_tag="VEL74_03550"
     CDS             complement(4910..5884)
                     /locus_tag="VEL74_03550"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015201793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYG61631.1"
                     /translation="MEYTRLGRTGLKVSRVCLGCMSYGDPAWRDWVLSEEASRPFIQR
                     ALELGINFFDTADMYSRGVSEEVVGRALRDFAQRDEVVLATKVFFPLGGGVNGKGLSR
                     KHILASIDASLRRLGTDYVDLYQIHRWDYETPIDETLEALHDVVRAGKARYVGASSMF
                     AWQFAKALYLADLRGWSRFVSMQNHYNLLYREEEREMLPLCREEGIGVIPWSPLARGV
                     LAAGRRGTGGDTTRAKSDDYTQRLYEGADFEIVDRVVSVAEARGLSPARIALAWLLHQ
                     PGVTAPIIGATKMHHLEDAVAAVKIHLDPEERARLEEPYRPRPVLEHQ"
     gene            5998..7539
                     /locus_tag="VEL74_03555"
     CDS             5998..7539
                     /locus_tag="VEL74_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYG61632.1"
                     /translation="MSGETKLEGPDLAQGIPLWVLADGGQILGHTGGEAVLVVRRGEE
                     IFAIGASCTHYGGPLAEGLVVGDTVRCPWHHACFSLRTGEALRAPALNPASCYRVERE
                     TDRIFVREKLEAAPRQAAQAPDPAVILGSGAAGNAAAEELRRQGYEGRILLVGPELHL
                     PYDRPNLSKDYLAGNAPEEWIPLHPWDFYGERRIELIQGARATAIDPAAKTVTLDDGR
                     TLSWGALVLATGADPVRLALPGGDLPHVHYLRTLDGSRRIIDAAKTAGHAVVLGASFI
                     GLEAAAALRARGLAVTVVAPDERPLGKIMGPEIGDFIRALHEEHGVVFHLGTTAQTIG
                     RDEVRLATGETLPADLVLIGVGVRPNVALAEAAGLAVDNGVLVDEYLETSAPGIFAAG
                     DIARWPDPHSGERLRIEHWVLAERQGQAAARNVLGRRERFDAVPFFWSQHYDVAINYV
                     GHASRWDALEVAGSIPGRDACVAFHDRGRIAAVATIYRDKESLEAELAMERDVQARHT
                     PANQG"
     gene            complement(7515..8234)
                     /locus_tag="VEL74_03560"
     CDS             complement(7515..8234)
                     /locus_tag="VEL74_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYG61633.1"
                     /translation="MTERRLSRRDLFRLGLVAVPASLAAACGWDGGAVLGPRLRSFSR
                     VNDWLGEKLLAGERPAPQYPVSERTPDRHFPGYAQTPGYPRLADPAGWALAVGGLVRR
                     PTRLTLEMLQAMPRVTYTVKHHCVEGWTAIGTWTGVPVSAVAALAEPTDAARYLRFDS
                     FDRGYHTGWDLKSAMHPQTILAYAFNDRMLDPRRGAPLRLYSPVKLGYKLTKYLTGMT
                     FTAERAGGFWEDRGYPWFAGV"
     gene            complement(8227..8967)
                     /locus_tag="VEL74_03565"
     CDS             complement(8227..8967)
                     /locus_tag="VEL74_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015345765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HYG61634.1"
                     /translation="MEPAEQDEPVPEGMEKEEPVPAPPERRRLVKRHHWIVRAAHWAN
                     ALLLVGMIASGLQIYHAFRHFGPRGGPYLPNPWDGDLFPGWARLGGWLAGGLNWHFAL
                     AWPFVLTGLAYLIYMAVSGEWRSILFRPRDIPRAWQMQLYYLRLRKEHPPQGKHNALQ
                     KLAYTAIILLGALSVLTGFAIYKPVQLSWLVAAFGGFELARYWHFWAVWIFVAFTLVH
                     VLLVFVVDPASLRSMITGWYRGRFPSHD"
     gene            9039..10022
                     /locus_tag="VEL74_03570"
     CDS             9039..10022
                     /locus_tag="VEL74_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYG61635.1"
                     /translation="MTMRAIVATRLGEPEVLELSAAPRPEPGPGQVLVRVHRVGVNFS
                     DTERRRAVYRQPNLPWIPGGEGAGVVEAVGEGVDAALRGRRVGFFKPPTESGAYAEYA
                     VAPASTLFFLSDRLSFDEGAALPLQGLTAYGLVRIAARIEAGQTVLIHAAGGGLGLLA
                     VQLCRLAGARVLGTASTEAKAEAVRAMGGEPLAYGDDLVERVFAATARRGVDVVLDSV
                     GQATQDASLAMLAPYGHLVFYGDASGPPRPIDPDRLYQLSLKVSAFTLEQSRHPELWE
                     TARRDLLRWVEDGSLRLTIARVLPLAAAAEAHRLIESRQVAGKLLLDVNGE"
     gene            10066..10710
                     /locus_tag="VEL74_03575"
     CDS             10066..10710
                     /locus_tag="VEL74_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HYG61636.1"
                     /translation="MLNDSAFVQLLGLMGVAVFAVSGAISAGRKGFDLLGVVIIAVVT
                     AVGGGTVRDVLLDRHPIFWLAEPAHLVVILAAAGLTVIYVRFRKPPRGSLQIADALGL
                     ALFTISGTQIAEQRDLPGIAAVVLGAVTGSAGGLLRDLLSAEVPLLLRQSDLYATAAI
                     AGSSAYLLLQGIGVERMTAAFVGMATVVALRLAAILWRLRLPVFQVPDEGDEAR"
     gene            10707..11339
                     /locus_tag="VEL74_03580"
     CDS             10707..11339
                     /locus_tag="VEL74_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG61637.1"
                     /translation="MTPGGNPAFPLATPGDEDLLVELMREFYVIEHLPFDERVVRKGL
                     REILGHRAYGLIHLIQVDGHPVGYMVLTFGFSLEFHGRDALIDELYVQEEHRGRGLGR
                     VALELAEEVCRREGIAALHLEVDRVNVRAQEVYRKAGYRDHDRYLLTKWLDGSAQPSA
                     APQDPGTALKSCTSIVKELERRIGHASQPSSPSHQHTTTLIALTTASTSL"
     gene            complement(11820..>12064)
                     /locus_tag="VEL74_03585"
     CDS             complement(11820..>12064)
                     /locus_tag="VEL74_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61638.1"
                     /translation="GGDGGDGGDDDGLGGRVPVGFLQLYDEKAVDALNVVIHLLQAPE
                     SLAFVLEAAGGVTLERCGAILDERVAADGSDGAAAG"
BASE COUNT         1796 a         4304 c         4262 g         1702 t
ORIGIN      
        1 gatcctcgcc ctcctgatcc ttccggcgac cgccttcgcg gcggagcccg cccccgatcc
       61 ccggcagatc ctcgagcagg tgcgggaggc ctacaaagag ctctcctcct tccgcgagca
      121 cggcacggtc gaggtggccc cggccgaagg gagcacggag ccggcgcgcc agcgcctgtt
      181 cgagctggag gcgacgcctg gcgagctcac cgtcgttgtc gccgacgacc agaagggggg
      241 gcggcgcgac ctgctgtggc gtagcggcgg ccagttcttc cggtatgcga gcgacggcga
      301 gcagctccag ccggtccctt cgctcgccgc cggcctcacc ggcctgacca gcgagccgga
      361 gggggccgcc ttcatcgtcc cgcggctgct cagcgaagcg gacgccgcct ggccgggtgc
      421 cgagccggcc gctctcgaga aatccgaacg ctgcggcgcg ggcacctgtt gggtgatcgc
      481 cttcgctccg aaggacggcg tcgtctcccg gctctggatc gactccagga cctcgctgat
      541 caagaagtcc gagctgcgcg gcgcctcccg gacggtgacc gtgttcagcg aggtcgacga
      601 ggtggacggc gccgaggtgg gctcccgcat gtcctgggcc tcccccgggc agccggaccc
      661 ctggaaggac gtgaccctcg gcccgggcgg cgatttcagc gaggagatca ccgtcgagct
      721 gacgacggcc acggtgcgcg tggtggaccc ctacggccag cccgtcaccg gcctgcggcc
      781 cgaggacttc ctcgtcctgg tcgccggccg tgaggtcccc gtggcgtccg tcgactgggt
      841 cacctcgaag gccggcccgg cgccggcgga ccaggtcgcc cccggcgcga gcccggcgcc
      901 cctggacgag agcgccgccg ccgcggggcc ggcgcagccg gcgccggcgg gcaagctcgt
      961 cgtcttcttc gtccaggccc acatggaccc ctcccgcttc cggggacaga tcaagctgcg
     1021 cgcgcacacc cgcaagctcc tggacaccct gggcgccgag gaccggctcg cggtcctctc
     1081 cttcgactcc catctcaagc tgcggcagga cgtcacacgc gaccgggagg cggtccaccg
     1141 ggcgatcgac caggggatga gcttcggtgg caagccgctg ccgcgccccc cggccggcga
     1201 cccggaaacg ctcgcggccc atctcgactt cgagcgggcg ctggcggcgg cctctccgga
     1261 ggcggggctg gccgtcgccg cgcaggccct ccgcttcctg tccggcgaga aggtgatcat
     1321 ctatctcggc tatgggctcg ggaagatggt cgccgatgac gtggtgctga ggagcgagta
     1381 caaagacgcc ctccaggcgc tgggcgaggc gcgcgcctcc gtcttcgtgc tcgacatcac
     1441 ccaggcggac ttccacagcc tggaggggag cctggagcag atcgcctacc agaccggcgg
     1501 cacctactcc aagaccctca tcttcgaaaa cctggccacc cagaccctgg cgaacgcgat
     1561 ctccggccac tacgtgctgg ccttcgacag cgacaccctc cctcgcggcg gcggcaccct
     1621 gcgcatcaag ctccgggacg ggaagaaggg gaggatcctc gtccggactc ccaaggcgca
     1681 gggcgggcgc tgagaggaga gatctcggca aagagtacgg atatactgaa acagaaccgc
     1741 tcgctgaggg cggctcgcga gcatggagag gtttatgaaa acgactccgc tcctgttcct
     1801 ccttctgtct ctctatcccc tgcaggcact gccggcggag cccggcgccg gcgagccgac
     1861 ggccctggag atcctccagc gggtacgaac cacctacgcc agcctcccct ccttccgtga
     1921 ggagggggtg atcacctcca caggcggtgc cggacgcgag ctccgtttcg agctcggcgc
     1981 ggcggacggg aaggtctcgc tctcggcctc cggcgacttc gactacgggg aagacgggat
     2041 ggacgacgag gagcggacgg ccctggccgt tcccgggctc ctcgcggaag ccggctggcc
     2101 cgggtcccgt agcgcgacgc tcgagaggca ggagccctgc ggcacctcga attgctgggt
     2161 catcgccctg acggagacgc cggagaaggc cgaggagggc gggggcgtcc tccgcctgtg
     2221 ggtcgacacc gcgacctact atctctggcg gaccgagctg cgcggaccgg gggaattcgc
     2281 gcgcaccctg gtggtcgagc acgagccgcg ggagttcgct ccgcgggcgg ccgccgggga
     2341 ggacctctgg gcctcggccg agaccgccgt gctctcgaag gtcacgctcc gggtgaccgg
     2401 cgccgacggc cagcccgtca ccggcctcaa gccccaggat ttccgggccg ccgtggccgg
     2461 tcaggagctc ccggtcgtct ccgtggactg ggtctcctcg cagccgaccg gccaggacgg
     2521 gacggcaccg ggagccggaa agctcgtgat catcttcgtg caggcccatc cgcagcgaac
     2581 ccggctcgcc ggccagatgc agttgcgcca gagtacccgc gagctcctcg gcaccctcgc
     2641 ccccgaggac cgggtcgccg tcgtctcctt cgactcccat ctgaagctcc ggcaggactt
     2701 caccgccgac cgggaggcgg tccaccaagc catcgaccgg ggcatgcgct tcggcggcga
     2761 gaccccggcg ccgcccggca acgccgagcc ggagacgctg gcggcccacc tcgacttcgg
     2821 ccaggcccga gcggcggcct ccccggagca tgcgctgctc gtcaccggcc gcgccctggc
     2881 gcggctgccc ggaaagaagg tggtcctctt catcggctat ggcctgggag agcacgtcga
     2941 gcgggaccgg gtggcggtgg agagccagat ggacgaagcc cttctcctcc tgttgcaggg
     3001 acagacttcg gtcttcgtgc tcgacgtcaa ccaggtggac cgtcacagca tgagggaagg
     3061 attggtccgg ctcgccacca acaccggcgg cacgtatgca agcacccgtc tctcgcccca
     3121 cctggcggtc cggagcctcg ccggcctggt ctcgggccac tacgtgctga ccttcgacgg
     3181 cgccaccatc cccctggaag gaggcacgct gcaggtcacg ctgagagacc cgaagcgggg
     3241 gaaggtcttc atcccgccgc tgttcctggc cggccggcct cagtaaggga aacgcacgga
     3301 ctgaaaggag cctccgaatg gccaagagag gtgaacgaat cctcgccgtt tcttcggccg
     3361 cgctgacggc ggtcttcgct tggatggtgc tcagcgtcgt gccggccttt gccgagagcg
     3421 cccaggagat cctccagcgg gtgcggaccg cctatgccgg cctcccctcg ttcagcgagg
     3481 cggggatcct cacggaggtc acctccgagg gaggcgccga gcagagacat gagatccggt
     3541 tcgagctcgc cgtggcggga ggggaggtct ggctcacggt gcccctggac tcggagggcg
     3601 gaaaagaacg gttgggcgac caggagcggg cggcgctggc ggtccccggg ctcctcgcgg
     3661 acgctacctg gccgggcttc cgccaggctt cgctcgaggg gcaggagccc tgcggcgcgt
     3721 cggcctgctg ggtgatctcg ctgacagagg agacgaagcc gggcggagag atcctccggc
     3781 tctgggtcga caccgagacc tcctacgtct ggaaaaccga gctgcggatc ccgggggaag
     3841 cggcgcggac cctggtcgtc gagcacgagc cgcgggagtt cggcccgctg accggggacg
     3901 acctctgggc cacggccacc gctcaggaag ggcagagtac cctcgaaggc ttcagcgagg
     3961 agatcaccgt cggcctgtcg acggccacgc tctgggtggt ggatcaccag ggccggcccg
     4021 tcaacggcct ccgtcccgag gacttccggg ccgtcctggc cggccgggag atcccggtcg
     4081 ccgccgtgga ctgggtctcc tccaggcaga ccggccagga cggggcgggg gcggcaccgg
     4141 cgccgggcaa gctcatcgtc ttcctggtgc aggccagccc cgtgaacgtg tttctctcgg
     4201 ggcagatgcg gctgcgccgg cagatccgcg agctcctcga caccctcgct cccgaagacc
     4261 gggtcgccgt cctctccttc gactcccatc tcaagctctg gcaggacttc accctcgacc
     4321 gggacgcggc ccaccgcgcc ctcgaccggg gcatgctgtt cggcggagac agcccggtgc
     4381 caccgcgcct ggccgagccg ggcacgctgg cggctcattt cgatctggaa caggcgcggg
     4441 cggcggcctc ggtcggacgg gcgttggacc ttaccggccg ggccctcctg ccgctacacg
     4501 gagaaaaagt ggtcgtcttc ctgggttacg ggatgggaga gagccgcagg gacgacaaag
     4561 aggtgctcgc ggcagccctg gcctccctgc agcaggcgcg ggccccggtc ttcgtcctcg
     4621 acatcgtcca ggggagcagt cactccctgg tatcgagcct gctgaagctc gccgcccaca
     4681 ccggcggtac ctatgccaag acctatttca cgccggacct agccacccgg aaactggccg
     4741 gtctgttctc gggccattac gtgctgtcct tcgacagcgg caccatccag ggtggcggca
     4801 ctctgcaggt cactctgaag gactcgagac gggggacggt cctcgcccgg tccgtcagcc
     4861 cggtcgaccc ggacgcctca gacgggcgct gactactggt gctcgagcac tactgatgct
     4921 cgagcaccgg gcgcggccgg tagggctcct cgagccgcgc ccgctcctcg ggatcgaggt
     4981 ggatcttgac cgccgccacc gcgtcctcca ggtgatgcat cttggtggct ccgatgatcg
     5041 gcgcggtgac gccgggctgg tggagcagcc aggcaagagc gatccgtgcc gggctcaggc
     5101 cccgcgcctc ggccacggag acgacgcggt ccacgatctc gaagtcggcc ccctcgtaga
     5161 ggcgctgggt gtagtcgtcg ctcttcgccc gggtggtgtc gccgccggtg ccgcggcggc
     5221 cggcggcgag gacgccccgg gccagcgggc tccaggggat gacgccgatc ccctcctcgc
     5281 ggcagagggg gagcatctcg cgctcctcct cccggtagag gaggttgtag tggttctgca
     5341 tcgagacgaa gcgcgaccag ccccgcaggt cggccaggta cagcgccttg gcgaactgcc
     5401 aggcgaacat gctggaggcg ccgacgtagc gggccttgcc ggcgcgcacc acgtcgtgga
     5461 gcgcctccag ggtctcgtcg atcggcgtct cgtagtccca gcgatggatc tggtagaggt
     5521 cgacgtagtc cgtgccgaga cggcggagcg aggcgtcgat cgacgccagg atgtgcttgc
     5581 gggagagccc cttgccgttg acgccgccgc ccagcgggaa gaagaccttg gtggcgagga
     5641 ccacctcgtc gcgctgggcg aagtcgcgca gcgcccggcc gaccacctcc tcgctcaccc
     5701 cgcgggagta catgtcggcg gtgtcgaaga agttgatccc cagctccagg gcgcgctgga
     5761 tgaacgggcg gctcgcctcc tcggagagca cccagtcgcg ccaggcggga tcgccatagc
     5821 tcatgcagcc gagacagacg cgggagacct tgaggccggt gcggccgaga cgggtgtatt
     5881 ccatggctcc ctatcttggc agatcggcgg ccgctgtaac ccttgggccc tccgtgactc
     5941 tctaggtaga aaggaccccg gcgtggtcac gccgggtctg tcagaggagg aggcgagatg
     6001 agcggagaga cgaagctcga gggacccgat ctggcgcagg gcatccccct gtgggtcctc
     6061 gcggacggcg ggcagatcct tggccacacg ggcggcgagg ccgtcctggt ggtgcggcgg
     6121 ggcgaggaga tcttcgccat cggcgcgagc tgcacccact acggcggccc gctggccgag
     6181 ggactggtgg tgggcgatac ggtgcgctgc ccgtggcacc acgcctgctt cagcctgcgg
     6241 accggcgaag cgctccgcgc tcccgccctg aatcccgcct cctgctaccg ggtggagcgc
     6301 gagacggacc gcatcttcgt ccgcgaaaag ctggaggcgg caccgcggca ggcggcgcag
     6361 gcccccgatc cggcggtcat cctggggagc ggcgcggcgg ggaacgccgc cgccgaggag
     6421 ctccggcggc agggctacga ggggcggatc ctcctcgtcg gccccgagct ccacctcccc
     6481 tacgaccggc ccaacctctc caaggactac ctcgccggca acgcccccga ggaatggatc
     6541 cccctgcacc cctgggactt ctacggcgag cggcggatcg agctgatcca gggcgcgcgg
     6601 gccaccgcca tcgatccggc ggcgaagacc gtcacgctcg acgacggccg gaccctctcc
     6661 tggggcgcgc tggtcctcgc caccggcgcc gatcccgtgc ggctggccct gcccggcggc
     6721 gacctgccgc acgtccacta cctcaggact ctggacggca gccgcaggat catcgacgcg
     6781 gcgaagacgg ctgggcacgc ggtggtcctg ggggccagct tcatcggcct ggaggccgcg
     6841 gcggcgctgc gcgcccgcgg cctcgcggtg accgtcgtcg cgccggacga gcggcccctg
     6901 gggaagatca tggggccgga gatcggcgac ttcatccgcg ccctgcacga ggagcacggc
     6961 gtcgtcttcc atctcggcac cacggcccaa acgatcggcc gggacgaggt gcggctcgcc
     7021 accggcgaga ccctgccggc cgacctggtg ctcatcggcg tgggggtgcg gcccaacgtg
     7081 gcgctggccg aggccgccgg gctcgccgtc gacaacggcg tgctggtgga cgagtacctg
     7141 gagaccagcg cgcccgggat cttcgccgcc ggcgacatcg cccgctggcc cgacccccat
     7201 tcgggggagc gcctgcggat cgagcactgg gtcctagccg agcgccaggg gcaggccgcg
     7261 gcacgcaacg tcctcggccg gcgcgagcgc ttcgacgcgg tccccttctt ctggagccag
     7321 cactacgacg tggcgatcaa ctacgtcggc cacgcctcgc gctgggacgc cctcgaggtc
     7381 gcgggctcca tcccgggccg cgacgcctgc gtggccttcc acgaccgcgg ccggatcgcc
     7441 gccgtggcga cgatctatcg cgacaaggag agcctggagg ccgagctcgc catggaacgg
     7501 gacgtccagg cccgtcacac ccccgcgaac caggggtagc cgcggtcctc ccagaagccc
     7561 cccgcccgct ccgccgtgaa ggtcatgccc gtcaggtact tggtcagctt gtagcccagc
     7621 ttgaccggcg agtagaggcg gagcggcgcg ccgcggcggg ggtccagcat ccggtcgttg
     7681 aacgcgtagg ccaggatggt ctgcgggtgc atggcgctct tcagatccca gccggtgtga
     7741 taaccgcggt cgaaggagtc gaagcgcagg tagcgcgccg cgtccgtggg ctcggccagc
     7801 gccgccaccg ccgagaccgg caccccggtc caggtgccga tggcggtcca cccctcgacg
     7861 cagtggtgct tgaccgtgta ggtgacccgc ggcatcgcct ggagcatttc caacgtcaga
     7921 cgggtcggcc ggcgcaccag gccgcccacc gcgagggccc agccggcggg gtcggcgagg
     7981 cgcggatagc cgggcgtctg ggcatagccg gggaagtggc ggtccggcgt ccgctcgctc
     8041 accggatatt gaggcgccgg gcgctcgccg gcgaggagct tctcccccag ccagtcgttg
     8101 acgcgggaaa aggaccgcaa gcgggggccc agcaccgcgc cgccgtccca gccgcaggcg
     8161 gcggcgaggg aggcggggac ggccaccagg cccagacgga acaggtcgcg acggctgaga
     8221 cggcgttcag tcatggctcg ggaacctccc ccggtaccag ccggtgatca tggaccgcag
     8281 cgaggcgggg tccaccacga aaaccaggag gacgtggacc agggtgaagg cgacgaagat
     8341 ccagaccgcc cagaaatgcc agtagcgggc gagctcgaag ccgccgaagg ccgccaccag
     8401 ccaggagagc tgcaccggct tgtagatggc gaagccggtg aggacggaga gcgcccccag
     8461 caggatgatg gcggtgtagg cgagcttttg cagcgcgttg tgcttgccct gaggcggatg
     8521 ctccttgcgc agccgcaggt agtagagctg catctgccag gcgcgaggga tgtcccgcgg
     8581 ccggaacagg atcgagcgcc actcccccga gacggccatg tagatcaggt aggccaggcc
     8641 ggtgaggacg aacggccagg cgagggcgaa gtgccagttg aggccgccgg caagccagcc
     8701 gcccagccgc gcccaaccgg ggaacaggtc gccgtcccac gggttcggca ggtacgggcc
     8761 gccgcgcgga ccgaagtgcc ggaaggcgtg atagatctgc agaccgctcg cgatcatccc
     8821 caccagcagc agggcgttgg cccagtgcgc ggcccgtacg atccagtggt ggcgcttgac
     8881 cagccgccgg cgctccgggg gcgccggcac cggctcttcc ttctccatcc cctccgggac
     8941 gggctcgtcc tgttcagcgg gttccatgtc ccctccctcg ctatcgccag actatacggc
     9001 cggaggggag gggacggttt gctgataccc tccgccgcat gaccatgcgc gccatcgtcg
     9061 ccacccgcct cggggagccc gaggtgctgg agctgtccgc agccccccgt ccggagccgg
     9121 gcccggggca ggtgctggtc cgggtccacc gggtcggcgt caacttctcg gatacggagc
     9181 gccgccgcgc cgtctaccgg cagccgaacc tcccctggat tcccggcggc gagggggccg
     9241 gcgtcgtgga ggcggtgggc gagggggtcg acgcggcgct ccgcggccgg cgcgtggggt
     9301 tcttcaagcc gccgacggag agcggcgcct acgcggagta cgccgtggcg ccggccagca
     9361 cgctgttctt cctgagtgat cgcctcagct tcgacgaggg ggccgccctg ccgctccagg
     9421 ggctcaccgc gtacggcctg gtgcggatcg ccgcgcggat cgaggcgggc cagacggtgt
     9481 tgatccacgc ggccggcggc gggctcgggc tgctcgccgt ccagctctgc cgcctcgccg
     9541 gcgcgcgggt tctcggcacc gcttcgaccg aggccaaggc agaggccgtc cgcgccatgg
     9601 gcggcgagcc cctggcctac ggcgacgacc tggtggagcg ggtcttcgcc gcgactgctc
     9661 gccgcggcgt cgacgtggtg ctcgactcgg tgggacaggc cacccaggac gcgagcctcg
     9721 cgatgctcgc cccctacggc catctcgtct tctacggcga cgccagcggc ccgccgcgcc
     9781 ccatcgaccc ggaccgcctc taccagctct ccttgaaggt cagcgccttc acgctcgagc
     9841 agagccgcca tcccgagctc tgggagacgg cgcgccggga cctgctgcgc tgggtcgagg
     9901 acggctcgct gcgcctcacc atcgcgcgcg tcctcccctt ggccgcagcg gccgaggccc
     9961 accggctcat cgagtcgcgc caagtggccg gcaagctgct gctcgacgtc aatggagagt
    10021 agcggcggag agtagggccg cccgccggga gaggggagca ccgcgatgct caacgactca
    10081 gccttcgtcc agctcctggg cctcatgggc gtcgccgtct tcgcggtgag cggcgccatc
    10141 tcggccggga ggaagggctt cgacctgctg ggggtggtga tcatcgccgt ggtgacggcg
    10201 gtgggcggcg gcacggtccg cgacgtcctg ctggaccggc accccatctt ctggctggcg
    10261 gagccggcgc acctggtcgt gatcctcgcc gccgccgggc tgacggtgat ctacgtgcgc
    10321 ttccgcaagc cgccgcgggg ctcgctgcag atcgcggacg ccctcgggct cgccctgttc
    10381 accatcagcg gcacccagat cgccgagcag cgggacctgc cggggatcgc cgccgtggtc
    10441 ctgggcgccg tcaccggctc ggcgggcggc ctgctgcgcg acctcctgag cgccgaggtt
    10501 ccgctgctgc tacgccagag cgacctctac gccaccgccg ccatcgccgg ctcgtccgcc
    10561 taccttctcc tccaggggat cggagtggaa cggatgacgg ccgccttcgt ggggatggcg
    10621 accgtcgtcg ccctgcgcct cgccgccatc ctctggcggc tgcggctgcc cgtcttccag
    10681 gtgcccgacg agggggacga ggcccgatga ctccaggagg aaacccggcc tttcccctgg
    10741 ccaccccggg cgacgaggat ctcctcgtcg agctgatgcg ggagttctac gtcatcgagc
    10801 atctcccctt cgacgagcgg gtggtgcgga agggcctgcg ggagatcctg ggccaccgcg
    10861 cctacggcct catccacctg attcaagtgg acggccaccc tgtgggctac atggtgctca
    10921 ccttcggctt cagcctggag ttccacggcc gggacgccct gatcgacgag ctctacgtcc
    10981 aggaggagca ccgcgggaga ggcctgggcc gggtcgccct ggagctggcc gaggaggtct
    11041 gccgccgcga ggggatcgcg gcgctgcatc tcgaggtgga ccgggtcaac gtccgggcgc
    11101 aggaggtcta ccgcaaggcg gggtaccggg accacgaccg ctacctgctg accaagtggc
    11161 tcgacggctc agcacagcca agcgcggcgc cccaggaccc gggcaccgcg ctcaaatcgt
    11221 gcacatcaat tgtcaaagaa ctggagagga ggattggcca tgccagccaa ccctcctctc
    11281 cgagccatca acataccaca acgttgattg cattgactac ggcatcgact tctttgtagc
    11341 cttcgccctg cgactcagga ggaggtcgcc gatccagacg cctctctccg gcctgctgct
    11401 ctttgacaac tgaaccgtga cggggcgccg ccgtgtccaa ggccgcgccc ctgaggactt
    11461 cctccgtccg acaggaggat cgccaagagg cggaggcccg gatgcctccg cctctcgagg
    11521 ctggctccct gccccgcaaa caacggccat acccctcgaa aggaagacca tccccgtggc
    11581 aacgagtcca tccccaggga aacgttcgcc cttcctgcgg gtacggccac cctaccccgg
    11641 gtaggaaggt catccccgcg ggcgcggcca tcaccccgtg ggtacggccg tcccactcgc
    11701 ggggatgacg gccctacctc gggtacggca aacgtttccc tgggtacgga ggcgtaggaa
    11761 cgagaggtgc gccaccggaa ggactgaacc taccgatggc gcctatacgt agaggatgtc
    11821 tatccggcgg ctgccccgtc cgagccgtcc gccgccaccc gctcgtcgag gatggcgccg
    11881 caccgctcga gggtgacccc gcccgccgcc tccagcacga aagccagcga ctccggcgcc
    11941 tggagaagat ggatgacgac gttcagcgca tcgaccgctt tctcgtcgta gagctgaagg
    12001 aagccgaccg gaacacgacc cccgagaccg tcgtcgtcgc cgccatcgcc gccatcgccg
    12061 ccat
//
LOCUS       DASPJI010000127         2705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_350821, whole genome shotgun sequence.
ACCESSION   DASPJI010000127 DASPJI010000000
VERSION     DASPJI010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350821"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2705
                     /locus_tag="VEL74_03590"
     CDS             <1..>2705
                     /locus_tag="VEL74_03590"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HYG61639.1"
                     /translation="AEATWRRGETPLARAEVNVRPEGPGEAPPVLLDVHADLLPASTG
                     RRHVEGTLRAESWETLAEATAQPLKAEVQSADVAATLAQVRSLWPRLVPQLPEGTPAR
                     GTLKADLRLTGPLPSPDATLAADWSPEPGSHLRVTGSGKPATWTGRAEAVAERLPLSI
                     ASPFLPGLAGEVTGQVQISGAPGAYRADAALTAANAAYPPSVQRLESARIDARGTLGG
                     RPLAWNGTVSVDGAGLFAAPNASGTARVESFNLLADGRLVPSPLAWRGKVTLDGQGID
                     AEGIARASRIEAVADGTVGSSPLTWNGTVSLNGQDVDAPGTARAARVALVADGRLRPD
                     PFAWNGTLTLDGQDVEAPETARAEVVRAEVVRAEVTGNLSGPSLTRLSGTASATVEAP
                     RLELTGAETSIEGLQLDATVEGKELRIASLSGSLPEGRAFNASGRATLSPLLAEADLD
                     LSLVRPVDAIPAADLTASLRQGVLTVLAPRVETASGPVRLDATVPLGALRGVPTLAKA
                     IASLPFEPAPGLVSVSLDAPALDTAALLPSLGMEAGEERARGGLTASFTFDPAAPAAG
                     RGEVRLTDLVVETPQGRAAAQGAVTARLADGRLEILPARLAVEGSGISGAGVDLRGAA
                     DLDPAWSPWTDPPASVVRRMSADAGGTLEASLLQPFLQGGTAAGALTFSATASGPLDG
                     LTGSVRASGPEASFFWPTPYATRVEAPEVVIALRQGSWQIEEGRARLNGGAVNLTGGG
                     AIGGDDGGFILGAGLEDVRYRVDYGLSVLVDGQLSLTMPPEGRSLLAGQVVLDRGVLD
                     RDVNLDREVLNTLLAPEDTPGTEASVLDEIDLDVRVSTTEGVRIKNNVGDLRASWDTL
                     RITGTAENPVIRGRIDLDPGGLLYAYGQTVRIDRGSLLFRGDPLN"
BASE COUNT          316 a         1118 c          922 g          349 t
ORIGIN      
        1 gccgaggcga cctggcgccg cggcgagacc cctctcgcgc gggccgaggt gaacgtccgg
       61 ccggagggac cgggcgaggc cccaccggtc ctcctggacg tccatgccga cctcctcccc
      121 gcctcgacgg gacgccggca cgtcgagggc acgctccggg cggagtcctg ggagacgctg
      181 gccgaggcca ccgcccagcc gctgaaggcc gaggttcagt ccgccgacgt cgccgccacc
      241 ctggcccagg ttcgctccct ctggccacgc ctcgtccccc agctccccga ggggacgcct
      301 gcacgcggca cactcaaggc ggatctccgc ctgaccggcc cgctcccctc ccccgacgcg
      361 accctcgccg ccgattggtc gcctgagccg ggttcgcacc tccgggtgac gggctcgggc
      421 aagccggcga cgtggaccgg ccgcgccgag gccgtggccg agcggctgcc gctgtccatc
      481 gcctcgccct tcctgcccgg cctcgccggc gaggtcacgg gacaggtcca gatctccggc
      541 gcgccgggcg cctaccgcgc cgatgcagcc ctcaccgccg ccaacgccgc ctatccaccc
      601 tcggtgcagc gcctggagtc tgcccgcatc gatgcccgcg gcacccttgg tggtcggccc
      661 cttgcctgga acggcacggt ctccgtggac ggcgccggcc tcttcgccgc ccccaacgcg
      721 tccggcaccg cccgggtgga gagcttcaac ctcctcgccg acggccggct cgtcccctcc
      781 cccctcgcgt ggaggggcaa ggtcactctc gacggccagg ggatcgacgc cgaggggatc
      841 gcccgcgcct cccggatcga ggccgtggcc gacggcaccg tgggctcctc ccccctgacc
      901 tggaacggca cggtctccct gaatggccag gacgtcgacg cccccggcac cgcccgcgcg
      961 gctcgagtcg ccctcgtcgc cgacggcagg ctccggcccg atcccttcgc gtggaacggc
     1021 accctcaccc tggacggcca ggatgtcgag gcgccggaga ccgcccgcgc cgaggtcgtg
     1081 cgcgccgagg tcgtgcgcgc cgaggtcacc gggaacctct ccggcccgtc cctcacccgc
     1141 ctctcgggca ccgccagcgc caccgtggag gcgccgcggc tggagctgac cggcgccgaa
     1201 acgtccatcg aggggctcca actcgacgcc accgtcgagg gcaaggagct gcggatcgcc
     1261 tccctcagcg gctcgctgcc ggaaggccgg gccttcaacg cctccggccg cgccaccctc
     1321 tcgccgctcc tcgccgaggc cgacctcgac ctgagcctcg tccgtcccgt ggacgccatc
     1381 cccgcggccg acctcacggc ttccctgcgg cagggggtcc tcacggtcct ggccccccgc
     1441 gtggagacgg cctccggtcc cgtccggctg gacgccactg tccccctcgg tgccctgcgc
     1501 ggcgtgccga ctctcgccaa ggccatcgcc tccctcccct tcgagcccgc gcccggcctg
     1561 gtctcggtga gcctcgacgc cccggccctc gacacggcgg ccctcctccc ctccctcggc
     1621 atggaagccg gcgaggagcg ggcccgcggc ggcctcacgg catcgttcac cttcgacccc
     1681 gcggcgcccg cggccggccg gggcgaggtc cggctgaccg atctggtggt cgagacgccg
     1741 cagggacgtg ccgccgccca gggcgcggtg accgcccggc tcgcggatgg ccggctggag
     1801 atcctcccgg cccggctcgc cgtcgaaggc agtgggatct ccggcgccgg cgtcgacctc
     1861 cgcggcgccg ccgacctcga tcccgcctgg agcccgtgga ccgatccgcc ggcgtccgtg
     1921 gtccgccgca tgtccgcgga cgccggcggc acccttgaag cctccctcct ccagccgttc
     1981 cttcaggggg gcacggcggc cggcgcgctc accttctcgg ctacggcttc gggccccctc
     2041 gacggcctca ccggcagcgt gcgggcgagc ggcccggagg cctcgttctt ctggcctacc
     2101 ccgtacgcca cccgggtgga ggcgccggag gtggtgatcg ccctgcgcca ggggagctgg
     2161 cagatcgagg aggggcgggc gcgcctgaac ggcggcgcgg tcaacctcac cggcggcggc
     2221 gctatcggag gggatgatgg cggcttcatc ctgggcgccg gcctggagga cgtgcgctac
     2281 cgggtcgact acggcctctc cgtcctggtg gacggccagc tcagcctcac catgccgccc
     2341 gaggggcgct ccctcctcgc cggccaggtg gtgctcgacc gcggcgtcct cgaccgggac
     2401 gtcaacctgg accgggaggt tctcaacacc ctcctggcgc cggaggacac ccccggcacc
     2461 gaggcgtccg tcctcgacga gatcgacctc gacgtgcggg tgagcaccac cgaaggggtg
     2521 cggatcaaga acaacgtcgg cgacctccgg gcctcgtggg acaccctgcg gatcaccggc
     2581 acggcggaga accccgtcat ccgcggccgc atcgacctcg atccgggcgg tctcctgtac
     2641 gcgtacggcc agaccgtgcg catcgaccgc ggctcgctgc tcttccgggg cgacccgctg
     2701 aacga
//
LOCUS       DASPJI010000128         9063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_298405, whole genome shotgun sequence.
ACCESSION   DASPJI010000128 DASPJI010000000
VERSION     DASPJI010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9063)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9063
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298405"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..7376)
                     /locus_tag="VEL74_03595"
     CDS             complement(<1..7376)
                     /locus_tag="VEL74_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61640.1"
                     /translation="MTPRRSPTGPDIVRRRAAEHPSRPAFTFLDSAGAEAETLTRSEL
                     EARSLAVAAALQESGAVVSRGERVLLLHPPGLGFITSFLGCLYAGAVAVPAYPPGRNR
                     PAARLRSIAADARPRVVLAPSNLKPGAVTGRVAELADALWIATDTLEAALAGAWRAIS
                     PGPNDPAFLQYTSGSTSDPKGVVVTHGSLAHNEEAIRRAFGQSESSVVVGWLPLYHDM
                     GLIGNVLQPLYTGARAVLMAPLSFLQRPALWLEAVSRYRATTSGGPNFAYDLCVRKVS
                     EEEKAGLDLASWEVAFNGAEPVRADTLERFAAAFASCGFRRGALFPCYGLAEATLFVT
                     GGSPAEPPVVRAVEGPGSRERVGCGRAAPDLEVVIADPEAHTPCEPGRVGEIWVAGPS
                     VAAGYWGRPEETARTFQARLADGRGPFLRTGDLGSLAGGELFVTGRIKDLIILRGRNI
                     YPQDVERTAEESHPSLRPGGGAAFSIERGGEERLVLLHEVERSAGADPATRTEALEAV
                     RRAVAEEHEVAVEEVVLLRGGTILKTSSGKIRRSACRAAYLTGELAVVARSGPSPDIE
                     LGSRVVVPPPLGGRAVGSGGGQEGGFSLAEEVARVLRRRVEEIEPEAPLTALGLDSLG
                     AAELRSAVESRMGATVSMTALLEGVSLRELEGLVRAAERGEAPALLGAEPDLPGEHPL
                     SEGQRDLWVLDRLNPKAAAGNLAGAAWLLGGIDGAALGRALILLTERHAALRTTFETR
                     EGEPVRRVHGRLAPDYREGRAGEEELPGLLVAEAYRPFELETGPLLRVRVWTLPDGRV
                     ALLLAVHHLIVDFASAAVLLRDLAALYDQERGGAPARLGHSGPGLTAWIRHREAALAG
                     PREERLRAFWHQRLAGELPVLDLPVDRSRPLSPSWRGRTGSRHVDGLAGALRQVGRGR
                     GATLFGSLLAGFQAVLHRWSGHDEVNEVLVGTPTSVRGGLHGEVGYFVNPVVLRLGLV
                     GEPGFADLAGQAGGAAVEAFEHGDYPIPRLARDLGAQRGAGRPLFQAMLALQPTRHPE
                     ERALAPFALGEDGARVALGGIELESIALDEIRAQVDLTLMAAETERGGLALALQLDAD
                     LFDEATAGRLLGHLTAFLSGAAADPARPVAEIEILSPAEHSQIATWSAGSPLTEPADV
                     CLHDLIARQTLLTPEAVAVESGGERLTYDELLRRSADLAARLRSLGVGPEVRVGVCIE
                     RGPRMVVGLLAVLEAGGAYVPLDPAYPAERLSLLLADSGARLALTSGETDVLLSGVDL
                     PRVRIDDRPETPGEPGVVIRPSPGNLAYLIYTSGSTGRPKAVAVEHRSAVALVRWARR
                     AFSDRELDGVLATTSIGFDLSVFELFVPLCWGGRVVFAGSAGSILDLPDLPPSTGVRL
                     VNTVPSALAEVLRSGGLPPSVETVNLAGEPLRRDLVQALYGAGVRRVVNLYGPSEDTT
                     YSTVAEQEPDSQGEPPIGRPVDGTWARVLDSDLRPVPVGVAGELFLGGAGLARGYLGR
                     PELTAERFVPSPFAEEGAGARLYRTGDRARWRADGELLYLGRLDHQVKIRGFRVEPGE
                     IEAVLASHPEVEQAVVLAQGEEGGRRLVAYVIPAGEGSVPDLRAWLEQRLPGSLVPSV
                     FVTLESFPRTPNGKVDRRALADLAPGPAEGPRTPPRTPLEAAMAALWTEVLEIPEVGV
                     HDRFFDLGGHSLLAVRLLARVRERLGVDLPLTAVFQAPTVAGLARLAAAAPPWRLPPP
                     VPLPRDGSPFPLSFAQERMWLLHRLDPESPAYHVAGEVRLTGSLDVAALAAALDDLVR
                     CHEALRTRFPEGEGGPVQVVEPVSPLALQVIDLSDLPEEVRSAEAERLALAMARRPFD
                     LAAAPPVRALLLRLDGDEHRLLLTLHHAAADEASLDILSRDLGAAYDAVSQGLPSPVA
                     PPPLQMADVAVWQRGRMAGEALAARLAWWEERLAGLPSLELPADRPAPAAPGWQGGTV
                     TRPLPDETAAALAAVAREAGATLFMVLLAGFEGLLARLSGAVDFAVGSPFSTRDRREL
                     EGVVGPLLNSLVLRADVAGDPPFRELVKRVREATVAAHERADLPFELLVERLRPERRA
                     GANPLFDVAFILHRPPVARHSAHIEMTPRALPTGAVKFDLALYALETDEGIELALEHA
                     ADRWDEATAIRLLGHLTTLLARAVTDPERRLSELLPLTPAERQATTVERPSAASGASR
                     TRLTPVEELLAGIWEDLLGVERPGPGDDFFALGGQSLLAARVVSRLRQALGVELPLRA
                     LFEEKTLAGMAARVESTRRAGPPYSGPPLVPVPREGGLPLSFGQERLWFLDRLDPGSP
                     VYNMPGAVRLVGDLEPERLRAALEGIAARHEVLRAAFPAPAGEPVQVARPAAGLPLPR
                     VDLSSLAPEARRAETLRRTTAEALCPFELETGPPLRATLLRLGEREHVLLLTVHHIAF
                     DGWSLGVLLRELAALYAGEGERLPELPVQYADFAVW"
     gene            7595..8872
                     /locus_tag="VEL74_03600"
     CDS             7595..8872
                     /locus_tag="VEL74_03600"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HYG61641.1"
                     /translation="MKLPLRTAIFWIHLALGLTAGLVILVMSVTGVLLMYERQITEWA
                     DRGYRAAPPSPGAERLPVETLLSRVLAARPEATPASVILQADPAAPATVSLGRRSQLF
                     VNPYTGEILGEGSQKVRKFFHAVTDWHRWLGAADDNRDGARAVTGASNLVFLFLVVSG
                     LYLWVPRKWVRTQLRNVTWFRGGLSAKARDFNWHNVFGFWMAIPLFFVVASAVVISYP
                     WAGNLIYRAVGEEPPVRSGPPGGQGAGPGRARAEGARREGGPAGERPSGERRGEEPGF
                     DRSVLQGLDPLWARAERQVADWQTLSLRLPESADAPVTFTINRGHRGRPDLRSQLTLD
                     RARGEVVTWEPFASQSLGRRLRTWTRWIHTGEAGGVLGQTLAGIASAAGAVLVWTGFA
                     LAWRRLVPKRQSKKQTLTAEEADTAAPSQAYEA"
BASE COUNT         1328 a         3451 c         3218 g         1066 t
ORIGIN      
        1 tgccagaccg cgaagtcggc gtactgtacc ggcagctcgg gcagcctctc tccttcccct
       61 gcatagaggg cggccagctc gcgcagcagc acccccaggg accagccgtc gaaggcgatg
      121 tgatggacgg tgagcagcag gacgtgctcg cgctcgccga gccgcagcag ggtggcgcgc
      181 aacggaggcc cggtctccag ctcgaagggg cacagcgcct cggccgtcgt ccggcggagc
      241 gtctcggccc gccgcgcctc cggggcgagg ctcgacaagt ccacccgggg caacggcagg
      301 ccggccgccg gacgcgccac ctgcacgggc tctccggcag gcgccgggaa ggcggcgcgg
      361 aggacctcgt gccgggcggc gatcccttcg agcgcggcgc gcagccgctc cggctccagg
      421 tcccccacca ggcgcaccgc cccgggcatg ttgtagaccg ggctcccggg gtcgaggcgg
      481 tcgagaaacc agaggcgctc ctgcccgaaa gagaggggca gccccccctc ccgtggcacg
      541 ggcaccaggg ggggcccact atacggcgga cccgcgcgcc gggtggactc cacgcgggcg
      601 gccatccccg ccagggtctt ctcctcgaac agggcacgca gcggcagctc gactccgaga
      661 gcctggcgca gccgggagac cacccgggcg gcgagaaggg actgaccgcc cagggcgaag
      721 aagtcgtccc ccggacccgg ccgctccacc cccaggaggt cttcccagat ccccgccagc
      781 agctcctcga caggcgtgag ccgggtgcgc gacgccccgg aggcggcgga cggcctctcc
      841 acggtggtgg cttggcgctc cgccggagtc agcggcagca gctcggagag gcgcctttcg
      901 ggatcagtca cggcgcgggc caggagggtg gtcaggtgtc ccagcaggcg gatggccgtg
      961 gcctcgtccc agcggtccgc cgcgtgctcc agcgccagct cgattccctc gtccgtctcc
     1021 aaggcataga gggcgaggtc gaacttcacc gcgccggtgg ggagagcccg cggcgtcatc
     1081 tctatatggg cggagtggcg cgctacaggc ggccggtgga ggatgaaggc tacgtcgaac
     1141 agtggattgg cgccggcccg ccgctccggc cgcagccgtt ccaccagcag ctcgaacggg
     1201 aggtcggccc gctcgtgcgc cgccaccgtc gcctcccgga cccgcttcac cagttcccgg
     1261 aacggcgggt cccccgcgac gtcggcgcgc agcaccagcg agttgaggag cggcccgacc
     1321 accccctcca gctcccgccg gtcgcgggtg gagaacggcg accccactgc gaagtccacg
     1381 gcaccggaga gccgggcgag gagcccctcg aaacccgcca gcaggaccat gaagagagtg
     1441 gcgcccgcct cccgggcgac ggcggcgagg gcggccgcgg tctcgtctgg caggggcctc
     1501 gtcaccgtgc ccccctgcca gccgggcgcc gccggggccg ggcggtccgc cggcagctct
     1561 agcgacggga gcccggccag ccgctcctcc caccaggcca gccgcgccgc cagagcctcc
     1621 cccgccatcc gcccgcgctg ccagacggcg acgtcggcca tctggagcgg cggcggcgcc
     1681 acgggtgagg gcagcccctg tgagacggcg tcgtaggcgg cccccaggtc ccgggagagg
     1741 atgtccagcg aagcctcgtc cgcggcggca tggtgcaggg tgagaaggag ccggtgctcg
     1801 tcgccgtcca ggcgcaggag gagcgcgcgc acgggcggcg ccgcggccaa gtcgaacggc
     1861 cggcgcgcca tcgcgagggc gaggcgctcc gcctcggcgc tccggacctc ctcagggagg
     1921 tccgagagat cgatcacctg gagtgccaga ggggataccg gctccaccac ctgcaccggc
     1981 ccgccctccc cctcgggaaa gcgggtgcgt aacgcctcgt ggcagcggac cagatcgtcc
     2041 agggccgccg ccagcgccgc gacgtccagt gagcccgtga gccgcacctc gcccgctacg
     2101 tgataggccg gagactccgg gtcgaggcga tggagcagcc acatccgctc ctgggcgaag
     2161 gagaggggga agggggagcc gtcccggggc agcggcaccg gcggaggcag ccgccagggc
     2221 ggggccgccg cggcgagacg ggcgaggcca gccaccgtgg gcgcctggaa gaccgccgtc
     2281 agcggcaggt ctaccccgag acgctcccgc acccgcgcca gcagccgcac ggcgaggagc
     2341 gagtgcccgc ccaggtcgaa gaaccgatcg tggaccccta cctccgggat ctccagcacc
     2401 tcggtccaga gggcggccat cgccgcctcc agcggcgtgc gcggcggcgt gcgggggcct
     2461 tcggccggcc ccggcgcgag gtccgcgaga gcccgccggt ccaccttgcc gttcggcgtc
     2521 cgggggaacg actccagggt gacgaagaca gaggggacca gcgagccggg caaccgctgc
     2581 tccagccagg cgcggagatc cgggacgctc ccctcccccg ccgggatcac ataggccacg
     2641 agccggcgcc cgccctcctc cccctgagcg aggaccaccg cctgctccac ctccgggtgg
     2701 gaggccagaa ccgcctcgat ctcccccggc tcgacacgga agccgcggat cttgacctgg
     2761 tggtccagcc gcccgaggta gagcagctcg ccgtccgccc gccaccgcgc ccggtccccg
     2821 gtccggtaga gccgggcccc cgcgccctcc tccgcgaagg ggctggggac gaaccgctcg
     2881 gccgtcagct ccgggcgccc gaggtagccc cgggccagac cggcgccgcc caggaacagc
     2941 tccccggcaa cccccaccgg caccggccgt aggtcgctat ccagcacccg tgcccaggtg
     3001 ccgtccaccg ggcgcccgat gggtggctcc ccctggctgt ccggctcctg ctcggccacg
     3061 gtcgaatagg tggtgtcctc cgaaggccca tagagattga ccacgcggcg taccccggcg
     3121 ccgtagagcg cttgcaccag gtcccgccgg agcggctccc ccgccaggtt gaccgtctcc
     3181 accgacgggg gcagcccgcc ggagcggagc acctcggcca gggcggaggg gacggtgttg
     3241 accagtcgca ctccggtgga cggcggcagg tcgggcaggt cgaggatgct gccggcgctg
     3301 ccggcgaaca ccacccggcc gccccagcag agggggacga agagctcgaa gaccgacagg
     3361 tcgaagccga tggaggtggt ggccagcacc ccgtccagct cgcggtctga gaaggcgcgg
     3421 cgggcccacc gcaccagcgc caccgcgctc cggtgctcga ccgccaccgc cttgggccgg
     3481 ccggtcgagc ccgacgtata gataaggtag gccaggttcc cgggcgaggg gcggatgacc
     3541 accccgggct cgccgggtgt ctcgggtctg tcgtcgatcc ggacccgcgg caggtccacg
     3601 ccggacagga ggacgtcggt ctcgccgctg gtgagcgcca gacgggcccc ggagtcggcg
     3661 agcagcaggg acagccgctc ggccggatac gccgggtcca gggggacata ggcgccgccc
     3721 gcctccagca ccgcgagcag ccccaccacc atccggggac cgcgctcgat acagaccccc
     3781 acccgtacct ccgggcctac gcccaggctc cgcagacgcg cggccagatc ggcggagcgc
     3841 cgcagcagct cgtcataggt cagccgctcc cctccggact cgacggccac ggcctcgggt
     3901 gtgagcaggg tttggcgggc gatcagatca tgcagacaga cgtccgccgg ctcggtaagg
     3961 ggggagccgg cgctccaggt ggcgatctgg ctatgctcgg ccggcgagag gatctcgatc
     4021 tccgccaccg gccgtgcggg atcggccgcc gccccgctca ggaaggctgt caggtgcccg
     4081 aggaggcgcc cggcggtggc ctcgtcgaag agatcagcat cgagctggag ggcgagggcg
     4141 agtcctcccc tctccgtctc cgcggccatg agggtgaggt ccacctgcgc ccggatctcg
     4201 tcgagggcga tggactccag ctcgatcccg ccgagcgcca cccgcgctcc gtcctctccc
     4261 agcgcgaacg gggccaaggc ccgctcctcg ggatgccggg tgggctggag ggcgagcatg
     4321 gcctggaaga gcggccggcc ggcgccgcgc tgggcgccca gatcacgggc taggcgcggg
     4381 atcgggtagt cgccatgctc gaaggcctcg accgcggcgc cgcccgcctg cccggccaga
     4441 tccgcgaacc ccggctcgcc gacgagcccg aggcgcagca ccacggggtt gacgaagtag
     4501 ccgacctccc cgtggagccc gccccggacc gacgtgggcg tgcccaccag cacctcgttc
     4561 acctcgtcgt gaccgctcca gcggtggagg actgcctgaa agcccgccag gagcgatccg
     4621 aagagggtgg ctccccggcc acgccccacc tgacgcagag cgccggccag accgtccacg
     4681 tggcggctcc ccgtcctccc gcgccagctc ggggaaagtg gccgggagcg gtccaccggc
     4741 aggtcgagga cgggcagctc gccagcgagg cgctggtgcc agaaggcgcg gagccgctcc
     4801 tcccgcggtc cggcgagcgc cgcctcccgg tgccggatcc aggccgtgag ccctgggccg
     4861 gagtgaccga ggcgcgccgg tgcgccgcca cgctcctggt catagagcgc cgccaggtcg
     4921 cgcagcagca ccgccgccga ggcgaagtcc acgatcaggt gatggaccgc cagcagcagc
     4981 gccacccgac catccggcag ggtccagacg cgcacgcgca gcagcggccc ggtttccagc
     5041 tcgaagggcc ggtacgcctc cgccaccagc agcccgggca gctcctcctc tcccgcgcgc
     5101 ccctcccgat agtcgggcgc cagccgccca tggacccgcc gcaccggctc gccctcccgg
     5161 gtctcgaacg tggtgcggag cgccgcgtgc cgctccgtca gcaggatgag ggcccgcccc
     5221 agcgccgcgc cgtcgattcc ccccaggagc caggccgctc ctgcgaggtt gccggccgcc
     5281 gccttggggt tcagccggtc cagcacccaa aggtcccgct gcccctcgga gagagggtgc
     5341 tcccccggaa ggtcgggctc ggcgccgaga agggccggag cctccccccg ttcggccgcc
     5401 cgcaccaatc cctccagctc ccgtaagctg accccctcca ggagagccgt catggaaacc
     5461 gtcgcgccca tccgggactc gacggcgctc cgcagctccg ccgctccgag ggagtccagc
     5521 cccagagcgg tcaggggggc ctccggctcg atctcctcca cccgccgccg caggacccgc
     5581 gccacctcct ccgcgaggga aaaacccccc tcctgacctc ccccgctccc taccgcccgc
     5641 ccacccaggg ggggaggcac caccaccctt gagcccaatt cgatatcagg cgaggggccc
     5701 gaccgcgcca ctaccgccag ctcaccagtc aggtacgctg ctcggcaggc ggagcgacgg
     5761 atcttgccgc tggacgtctt gaggatcgtg ccaccccgta gcagcacgac ctcctccacg
     5821 gcgacctcgt gctcctcggc cacggcgcgg cgcaccgcct ccagggcctc ggtccgggtg
     5881 gccggatcgg cgccggccga gcgctccacc tcgtgcagga ggaccaggcg ctcctcgccg
     5941 ccgcgctcga tggagaacgc cgcaccgccg cccggccgca gggaagggtg gctctcctcg
     6001 gccgtccgct ccacatcctg gggatagatg ttgcggccgc ggaggatgat caggtccttg
     6061 atgcggccgg tgacgaacag ctcaccgcca gcgagcgacc ccaggtcgcc ggtgcggagg
     6121 aaggggcccc gcccatcggc gagccgggct tgaaaggtgc gcgccgtctc ctcgggccgc
     6181 ccccaatacc cggcggcgac gctcggcccc gccacccaga tctcccccac ccggcccggc
     6241 tcgcagggag tgtgagcctc ggggtcggca atcaccacct cgaggtccgg ggccgcccgg
     6301 ccgcacccca cccgctcgcg gctccccggg ccttccacgg cgcggaccac cggcggttcc
     6361 gcgggcgatc ccccggtgac gaacagcgtc gcctcggcca gcccgtagca cgggaagagc
     6421 gccccgcgcc ggaagccgca ggaggcgaac gccgccgcga agcgctccag ggtgtcggcc
     6481 cgcaccggct cggcgccgtt gaacgccacc tcccaactcg ccaggtcgag ccccgccttc
     6541 tcctcctcgc tcaccttgcg gacgcagagg tcataggcga agttcgggcc gccgctggtg
     6601 gtggcgcgat aacgggagac ggcctccagc cagagggccg gccgctgcag gaaggacagc
     6661 ggcgccatca ggaccgcccg cgcgcccgtg tagagcggct gcaggacgtt cccgatgagc
     6721 cccatgtcgt gatagagcgg cagccagccc accaccaccg acgactccga ctggccgaag
     6781 gcgcggcgga tggcctcctc gttgtgcgcg aggctgccgt gggtgaccac cacccccttg
     6841 gggtccgagg tcgagcccga cgtgtactgg aggaacgccg gatcgttcgg accgggcgag
     6901 atcgcgcgcc aggcgccggc cagcgcggcc tccagggtgt cggtggcgat ccatagagcg
     6961 tccgccagct ccgccaccct gccggtgacc gcgcccggct tcaggttgga gggagcgagc
     7021 accacccgcg gccgggcgtc ggccgcgata gaccgcagcc gtgccgccgg ccggttgcgt
     7081 cccggtgggt aggcggggac cgccaccgcc ccggcataca ggcagccgag gaacgaggtg
     7141 atgaacccga gccccggggg gtggagcagc aggacccgct cccccctgga gacaaccgct
     7201 ccggattcct gaagagcggc ggccaccgcc agagagcgcg cctcgagctc ggagcgggtg
     7261 agggtctcgg cctccgcgcc cgccgagtcc aggaacgtga aggccggccg cgaggggtgc
     7321 tccgcggccc tgcggcgcac gatgtcgggt ccggtggggg aacggcgcgg ggtcatcggc
     7381 tcctctctta cagatccttc gggtgccagc gggccataca ggcgagccac tccccgatgg
     7441 aagaccgtcc gcagaagaaa ctcttcggct tattgagatt ccatctcttt ttgtattgac
     7501 aaacaggact ccctcggtcc agtatctcgc cgcgcacatt gagattcatt ctcaagaaga
     7561 gagagtagat gagaacaggg gacgttcgag agccatgaag ctgccgttgc gtactgcgat
     7621 tttctggatc cacctcgccc tgggcctcac ggccgggctc gtcatcctgg tcatgtccgt
     7681 gaccggcgtg ctcctgatgt acgagcggca gatcaccgag tgggccgacc ggggctatcg
     7741 cgccgcgccg ccgtccccag gcgccgagcg gctgccggtc gagaccttgc tctcgcgcgt
     7801 cctcgcggcc cggcccgagg ccaccccggc cagcgtcatc ctgcaggccg accccgcggc
     7861 gcccgcgacg gtgagcctgg gacggcgcag ccagctcttc gtgaacccct ataccggcga
     7921 gatcctcggc gagggctcgc agaaggtccg gaagttcttc cacgcggtca ccgactggca
     7981 ccgctggctc ggcgccgccg acgacaaccg cgacggcgcc cgcgccgtca ccggggcctc
     8041 caacctggtc ttcctgttcc tcgtcgtctc ggggctgtac ctctgggtgc cgcgcaagtg
     8101 ggtccgtacc cagttgcgga acgtcacctg gttccgcggc ggcctgtcgg ccaaggcccg
     8161 cgacttcaac tggcacaacg tcttcggctt ctggatggcg atccccctct tcttcgtcgt
     8221 cgcgagcgcg gtcgtcatct cctatccctg ggcgggcaac ctgatctacc gcgccgtcgg
     8281 cgaggagccg ccggtacgca gcggtccgcc gggaggccag ggggcggggc cgggccgggc
     8341 gcgcgccgaa ggcgcgcgcc gcgaaggcgg accggccggc gagcggcctt cgggcgaacg
     8401 gcgaggcgaa gagccgggct tcgaccgctc ggtcctccag gggctcgatc cgctctgggc
     8461 ccgcgccgag cgccaggtcg ccgactggca gacgctcagc ctgcggctgc ccgagtccgc
     8521 cgacgcgccg gtgaccttca cgatcaaccg cgggcaccgg gggcgtcccg acctgcgctc
     8581 ccagctcacc ctggaccgcg cccgcggcga ggtggtgacc tgggagccgt tcgccagcca
     8641 gagcctcggc cggcggctgc gcacctggac gcgctggatc cacaccggcg aggccggagg
     8701 cgtgctcggc cagaccctcg ccggcatcgc ctcggccgcg ggagccgtcc tggtgtggac
     8761 cggcttcgcg ctcgcctggc gccggctggt accgaagagg cagtccaaga aacagacatt
     8821 gacagcggaa gaggccgata cggcagcccc ctcccaggcc tacgaggcct gagcgagggg
     8881 gcccgcgagg ctccgcgtat cttttttgat tccgaggaga gacacatgca aggcagaggc
     8941 aagaagcacg gaaaggaccg ccggcagggg ccctcgcgcc cgaattggct gttcatgggc
     9001 gcgctggtca cctcgacggc gctcgggggc cgcgccacgg cgcaggccga catccagcgc
     9061 cac
//
LOCUS       DASPJI010000129         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_7902, whole genome shotgun sequence.
ACCESSION   DASPJI010000129 DASPJI010000000
VERSION     DASPJI010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7902"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1964)
                     /locus_tag="VEL74_03605"
     CDS             complement(78..1964)
                     /locus_tag="VEL74_03605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009595026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG61642.1"
                     /translation="MISSASPRPRRPRRIATLIILAWNRWDLTERCLATLLETDLTDA
                     EVLVVDNGSTDETPERLAEFAAQGSIRVLSLPQNLGFVRGNNAGIEAAEPGSDIVLLN
                     NDMEFTQADWLERLRACAAVPEVGVAGCRLVLPDRRLLHAGTYILPDTAWGQQIGSLE
                     KDIGQYPGTREVEGIVFAVAYIRREVIDAIGGLALDFQSYFEDTDYCLRARQAGWKTV
                     VCGDVTLVHNEHGSTQKDPQAMMRLFQGSREIFRKKWTAALEKHYRRELLWQSIMNFP
                     TGYAMSSREIVRGLEQEGVRMAYRYVYGPGTVFPVPEPEASGDYRLDVIRGRPVPQRP
                     PVSVVYGQGDVFERNEGKYKIGYTMLEVDGFPADWVRQANRMDEVWVPSEFNRQGFLR
                     SGLQRPVHVIPLGVDVNYFHPGVQAHPNPMGEMVFLSIFEWGERKEPWLLLKAFNEVF
                     SAKEPVRLLCKIMNRDGDIRLKEEIRRLRLRPSGGKISYLFNLEFPHYQLGSLYRSAD
                     CYVSISRGEGWNMPLMEAMACGLPTIATDWGAHTEFLHEGIGYPLRVRRTVPAVAKCP
                     YYAGYSWADPDPEHLRHLLRQVYEEREEARRRGAAAAAEMAERWTWGHVARRIADRLQ
                     TVGS"
     gene            complement(1957..2247)
                     /locus_tag="VEL74_03610"
     CDS             complement(1957..2247)
                     /locus_tag="VEL74_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61643.1"
                     /translation="MESEEQRTIARLEARVEALEAALERRSRELRLIQRHVCKRDLIL
                     ISRLLAGLPPLPFGAYEPAFWQETTALTPAEVEETLTDLWRSLAPVSRELDD"
     gene            complement(2251..2550)
                     /locus_tag="VEL74_03615"
     CDS             complement(2251..2550)
                     /locus_tag="VEL74_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61644.1"
                     /translation="MLDDARLRGRTEAETFDRELRERELRDREARLTRLAWGAEDQDP
                     LAALTSERTDRSGGSDVVWRLQQDVERLAGFHNAVLRSKAWKLIQLARRPFGRSW"
BASE COUNT          407 a          950 c          991 g          420 t
ORIGIN      
        1 ggccaggatc agcaggagga tcagcagcgg cggcaagccc ggcgacagcg aacggcggcg
       61 cggcgtggcc tgtccggtca tgaccccacg gtctgcaggc ggtccgcgat gcgccgggcg
      121 acatggcccc aggtccatcg ctccgccatc tcggccgccg cggcggcgcc ccggcggcgc
      181 gcctcctccc gctcctcgta gacctggcgc agcaggtgcc gcaggtgctc ggggtccggg
      241 tcggcccagg agtagccggc gtagtagggg cacttggcca ccgccggcac ggtccggcgc
      301 acccgcagcg ggtagccgat cccctcgtgc aggaactcgg tgtgcgcccc ccagtcggtg
      361 gcgatggtgg gcaggccgca cgccatcgcc tccatcagcg gcatgttcca cccctcgccg
      421 cggctgatcg acacgtagca gtccgccgac cggtagagcg agccgagctg gtagtggggg
      481 aactccagat tgaacaggta cgagatcttg ccccccgagg ggcgcaggcg caggcggcgg
      541 atctcctcct tgagccggat gtcgccgtcc cggttcatga tcttgcagag gaggcgcacc
      601 ggctccttgg ccgagaagac ctcgttgaac gccttgagca gcagccaggg ctccttgcgc
      661 tccccccact cgaagatgga gaggaagacc atctcgccca tcgggttggg gtgggcctgg
      721 acccccgggt ggaagtagtt gacgtccacg cccaggggga tcacgtgcac cgggcgctgg
      781 aggccggagc gcaggaagcc ctggcggttg aactcgctgg gcacccagac ctcgtccatg
      841 cggttggcct gccgtaccca gtcggcgggg aagccgtcca cctccagcat ggtgtagccg
      901 atcttgtact tcccctcgtt gcgctcgaag acgtccccct gtccgtagac caccgagacc
      961 ggcggccgct gcggcacggg gcggccccgg atgacgtcca gccggtagtc gccgctcgcc
     1021 tcgggctccg gcaccgggaa gaccgtcccg ggtccgtaga cgtagcggta ggccatccgc
     1081 accccctcct gctcgaggcc gcgcacgatc tcgcggctgc tcatcgcgta gccggtgggg
     1141 aagttcatga tcgactgcca gagcagctcg cggcgatagt gcttctcgag cgccgccgtc
     1201 cacttcttgc ggaagatctc gcggctcccc tggaagaggc gcatcatggc ctgggggtcc
     1261 ttctgggtgg agccgtgctc gttgtggacc agcgtcacgt cgccgcagac cacggtcttc
     1321 cagccggcct gccgcgcccg caggcagtag tcggtgtcct cgaagtagga ctggaagtcg
     1381 agcgccaggc cgccgatggc gtcgatcacc tcgcgccgga tgtaggcgac ggcgaagacg
     1441 atcccctcga cctcgcgggt acccgggtac tggccgatgt ccttctcgag cgagccgatc
     1501 tgctggcccc aggccgtgtc cggcaggatg taggtgccgg catggaggag gcgccggtcc
     1561 ggcagcacca gccggcagcc ggcgacgccg acttcgggca cggcggcaca ggcgcgcagg
     1621 cgctccagcc agtcggcctg ggtgaattcc atgtcgttgt tgagcaggac gatgtccgag
     1681 cccggctcgg cggcctcgat gccggcgttg ttgccgcgga cgaagccgag gttctggggc
     1741 agggagagga cccggatcga tccctgggcg gcgaactcgg cgagccgctc cggcgtctcg
     1801 tcggtggagc cgttgtccac caccaggacc tcggcgtccg tgaggtcggt ctcgaggagc
     1861 gtggcgaggc agcgctcggt caggtcccag cggttccagg ccaggatgat gagggtggct
     1921 atccgccggg ggcgccgggg gcgcgggctc gccgagctaa tcatccagct cccgggaaac
     1981 cggcgccagc gagcgccaga ggtcggtcaa cgtctcctcg acctcggccg gggtgagcgc
     2041 cgtggtctcc tgccagaacg cgggctcgta ggcgccgaag gggaggggcg gcaggccggc
     2101 cagcagccgg gagatgagga tgaggtcccg cttgcagacg tggcgctgga tgaggcgcag
     2161 ctcgcgggag cgccgctcca gggccgcctc cagggcctcc acccgcgctt ccagccgcgc
     2221 gatggtccgc tgctcttcgg attccatgat ctaccaggag cgtccgaagg ggcggcgggc
     2281 gagctggatg agcttccagg ccttggacct gaggaccgcg ttgtggaacc cggccaggcg
     2341 ctccacatcc tgctgcaggc gccagaccac gtcggagccc ccgctccggt cagtccgttc
     2401 ggaggtcagg gcggcgagcg ggtcctggtc ctcggctccc caggcgagac gggtgaggcg
     2461 ggcctcgcgg tcgcgcagct cccgctcccg cagctcgcgg tcgaacgtct cagcctcggt
     2521 ccggccgcgc aggcgggcat cgtcgagcat atcctcgcgc ctctccatgg tcctgttgac
     2581 gggtcaacgg ctgcaagccg gagccgggag cagacgcccc tgaggcccag acttgaggtc
     2641 acatcgcctg gtgactatac ttacgcgaac tcgggaaacg caaccgggcc ccgcgcgtca
     2701 aaccgcttgg tcgatcgtcc ggtcttaccg tccacccctt tccaagaggg aatcgttgag
     2761 ttacgagc
//
LOCUS       DASPJI010000130         3784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_809998, whole genome shotgun sequence.
ACCESSION   DASPJI010000130 DASPJI010000000
VERSION     DASPJI010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809998"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VEL74_03620"
     CDS             complement(<1..850)
                     /locus_tag="VEL74_03620"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HYG61645.1"
                     /translation="MDAEAFVERFLKPHYLFRPGQGLRRLRQALSHDTPGPRLACLPW
                     GLPIHIDTREVIGLSIWQFGLYDLAVSETLWRLLRPGDLAVDVGANIGAMTGLMALRT
                     GPAGEVLAFEPHPEVFAELLANLELFQRRYGFSPVLPCHAALGREPGTGFLHTDGEFA
                     SNRGTAHLGAGEDGLPVPVVTLDEVLAGRTAALMKLDVEGGELQVLAGAADSLRRGLI
                     RNVVYESFAAQSQELADVLRGYGYEIYSLGRRLLGLDVATGEPRIPPYEPPSALATRD
                     PEALLRL"
     gene            complement(852..2615)
                     /locus_tag="VEL74_03625"
     CDS             complement(852..2615)
                     /locus_tag="VEL74_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HYG61646.1"
                     /translation="MSRVLGVSAYHADASAAAVADGRFVAGVEEERFRRIKHWAGFPE
                     QALRFCLDELGGDIGEVSALAVSRQPRAYLFRKALLALSHPRSLRRAASRVRNLAQVS
                     SLEERVAATFGRSPKLHPVEHHLAHVASAFFCSPWEEAMCLTVDGFGDFVSTMMAVGR
                     GNCVEVLDRVCFPHSLGLFYTAITQYLGFHGFGDEYKVMGLAAYGEPGFAAKLRRVVP
                     ERSDGSFRLDLRYFRHLSEGVEMSWEDGEPVQGLVFTPALEELLGSARRPGDELTQRH
                     KDMAASLQQVYEERFFALVRALQRRTGLDRLTLAGGCAMNSLANGRLFDNTDVRDVYI
                     QAAAGDAGTALGAALYVHHAVLGAPRNGFVMEHCYWGPAYGQDEIRRQIAERIPESGG
                     RDGAWGEIRIETFQDEERLAADTAEALVRGEVVGWYQGRSEWGPRALGNRSILADPRR
                     GDMKDILNLKIKRRESFRPFAPSVLEERTGEWFTIHFPDPFMIKVYPIRPEKRPLIPA
                     VTHVDGTGRLQTVSKRTNPRYWMLIRAFEERTGVPMVLNTSFNENEPIVNRPAEALDC
                     FLRTRMDRLVLGDVVLTRKEA"
     gene            complement(2612..3472)
                     /locus_tag="VEL74_03630"
     CDS             complement(2612..3472)
                     /locus_tag="VEL74_03630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYG61647.1"
                     /translation="MKAPVTVLFTTRNEEVNIERSLASVHGFADQVFVIDSESEDRTV
                     EIARRYADEVLNLPYEHGRIIPWIFQWGLDNLPIRNEWILILEADQALTPELKAEMAA
                     LLARPDLRENGFYLRRRQIFRGRPLRFGGYGSKYLMKLFRRGRGELDPVEQDTRVYIQ
                     GPVGKLSAPIEEWNRKEDAILFYLQKHLRYADAFAREELERRRRDLPWKLTPRLFGTP
                     DERVLWLKTRYYRMPLFVRPFLYFLYRYFFLLGILDGKNGFVFHFLQAFWFRLIVDVR
                     LEELMTEETR"
     gene            complement(3477..>3784)
                     /locus_tag="VEL74_03635"
     CDS             complement(3477..>3784)
                     /locus_tag="VEL74_03635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG61648.1"
                     /translation="DVFIHPAREERWGVSVAEALACGLPVIASSRVGAARDLLTAGRN
                     GFVYQAGDDAELARRLDDALRLSPDSVRDENRAILARWDYAATWQGILRAAARLQKR"
BASE COUNT          609 a         1265 c         1373 g          537 t
ORIGIN      
        1 ggagacggag cagggcttcc ggatcgcggg tggccagagc gctcggcggc tcgtacggcg
       61 ggatccgcgg ctcgccggtg gcgacatcga gtcccagcag cctccggccc agagagtaga
      121 tctcgtagcc gtagccgcgc aggacatccg ccagctcctg gctctgggcc gcgaaggact
      181 cgtacaccac gttgcggatc aggccccgcc gcagcgaatc ggcggccccg gccagcacct
      241 gcagctctcc tccctcgacg tccagcttca tcagcgcggc ggtccgcccg gcgaggacct
      301 cgtccagcgt caccaccggc acggggagcc catcctcacc ggctcccagg tgggcggtgc
      361 cgcggttgct cgcgaactca ccgtcggtgt gaaggaagcc ggtccccggc tccctcccga
      421 gcgcggcatg gcaggggagg acgggagaga acccgtaccg tctctggaag agttccagat
      481 tggccaggag ctcggcgaag acctcgggat gcggctcgaa cgccaggacc tcgcccgcgg
      541 ggccggtccg cagggccatg aggccggtca tggcgccgat gttggcgccc acgtcgacgg
      601 cgaggtcgcc aggcctcagg aggcgccaga gggtctcgct cacggccagg tcataaaggc
      661 cgaactgcca gatggagagg ccgatcacct cgcgggtatc gatgtggatc ggcagacccc
      721 aaggcaggca ggccagccgc ggccccgggg tgtcgtggct cagcgcctgc cgcaggcggc
      781 ggagcccctg tcccggacgg aagagatagt ggggcttgag gaaacgctcg acaaaggctt
      841 cggcgtccat ctcacgcctc ctttcgggtg aggaccacgt ccccgaggac gagccggtcc
      901 atgcgggtgc ggaggaagca gtcgagagcc tccgcgggcc ggttgacgat cggctcgttc
      961 tcgttgaacg aggtgttgag gaccatgggc acgccggtcc gttcctcgaa ggcacggatc
     1021 agcatccagt accgtgggtt cgtccgcttg gacacggtct ggagccgccc cgtgccgtcc
     1081 acatgggtga cggcggggat cagcggccgc ttctctggcc ggatcgggta caccttgatc
     1141 atgaaggggt ccggaaaatg aatggtaaac cattctccgg tccgctcctc cagaaccgac
     1201 ggcgcgaagg gacggaacga ctcccggcgc ttgatcttga ggttgaggat gtccttcatg
     1261 tccccccggc gcggatcggc caggatggag cggttgccca gggcccgggg gccccactcg
     1321 gagcgcccct ggtaccagcc caccacctcg ccgcggacga gggcctcggc ggtgtcggcg
     1381 gccaggcgct cctcgtcctg gaaggtctcg atccggatct ccccccaggc gccgtcccgg
     1441 ccacccgact ccgggatgcg ctcggcgatc tgccggcgga tctcgtcctg tccgtaggcc
     1501 ggcccccagt agcagtgctc catgacgaag ccgttgcggg gagcgcccag gacggcgtgg
     1561 tggacgtaca gggcggcgcc gagggcggtc cccgcgtcgc cggccgcggc ctggatgtag
     1621 acgtcgcgca cgtcggtgtt gtcgaacagc cggccgttgg cgagcgagtt catggcgcag
     1681 ccgccggcga gcgtcagccg gtcgagcccg gtacggcgct ggagggcgcg caccagggcg
     1741 aagaagcgct cctcgtagac ctgctggagc gacgccgcca tgtccttgtg gcgctgggtc
     1801 agctcgtcgc cggggcggcg cgccgaaccc agcagctcct cgagcgccgg agtgaagacc
     1861 agcccctgga ccggctcgcc gtcctcccag ctcatctcca ccccctcgga gaggtgccgg
     1921 aaatagcgga ggtccagccg gaagctgccg tcggatcgct caggaaccac ccgccggagc
     1981 ttggcggcga acccgggctc gccgtaggcc gcgaggccca tcaccttgta ctcgtcgccg
     2041 aagccatgga agcccaggta ctgggtgatg gcggtgtaga agaggccgag cgagtgcggg
     2101 aagcagacgc ggtccagcac ctcgacgcag ttgccgcgcc cgaccgccat catggtggag
     2161 acgaagtcgc cgaagccgtc gaccgtcagg cacatcgcct cctcccaggg ggagcagaag
     2221 aaggccgacg ccacgtgggc gaggtggtgc tccaccggat ggagcttcgg cgagcggccg
     2281 aaggtggcgg cgacccgctc ctccagcgac gagacctggg ccaggttgcg cacccgggaa
     2341 gcggcgcggc gcaggctgcg cggatgggag agggcgagga gcgccttgcg gaaaaggtag
     2401 gcgcggggct ggcgcgacac cgccagggcc gagacctctc cgatgtcgcc gccgagctcg
     2461 tccagacaga agcgcagggc ctgctcgggg aagccggccc agtgcttgat gcgccggaag
     2521 cgctcctcct cgactccggc gacgaagcgg ccatcggcca cggccgccgc cgaggcatcg
     2581 gcgtgatagg cgctcacccc cagcacgcgg ctcatcgggt ctcctccgtc atcagctcct
     2641 cgagacgcac gtcgacgatc agccggaacc agaacgcctg caggaaatgg aagacgaagc
     2701 cgttcttgcc gtccaggatc cccagcagga agaagtaccg gtagaggaag taaaggaacg
     2761 gccggacgaa cagcggcatc cggtaatagc gggtcttgag ccagagcacc cgctcgtcgg
     2821 gggtgccgaa caggcgcggc gtcagcttcc aggggaggtc gcgccgccgc cgctccagct
     2881 cctcgcgggc gaaggcgtcg gcatagcgca ggtgcttctg cagatagaag aggatggcgt
     2941 cctccttgcg gttccactcc tcgatgggag cgctcagttt tccgaccggc ccctggatgt
     3001 agacccgggt gtcctgctcg acggggtcga gctcgccgcg cccgcgccgg aagagcttca
     3061 tcaggtactt gctgccgtag ccgccgaagc ggagggggcg tccccggaag atctggcgcc
     3121 ggcggaggta gaagccgttc tcgcgcaggt ccggccgggc gaggagcgcc gccatctccg
     3181 ccttgagctc cggggtgagc gcctggtcgg cctccaggat caggatccac tcgttgcgga
     3241 tcggcaggtt gtccagcccc cactggaaga tccaggggat gatccggcca tgctcgtagg
     3301 ggaggttcag cacctcgtcg gcgtagcggc gggcgatctc caccgtccgg tcctcgctct
     3361 ccgagtcgat gacgaacacc tggtcggcga agccgtggac gctggccagc gatctctcga
     3421 tgttgacctc ttcgttgcgg gtggtgaaga gaacggtcac gggggctttc aagcgttcac
     3481 cttttctgca agcgcgcggc ggcgcgaagg ataccctgcc aggtggccgc gtagtcccac
     3541 cgggcgagga tggcgcggtt ctcgtcccgg actgagtcgg gggagaggcg cagggcgtcg
     3601 tccaggcggc gggcgagctc ggcgtcgtcg ccggcctggt agacgaagcc gttgcggccg
     3661 gcggtcagga ggtcgcgggc cgctccgacc cgggacgagg cgatgaccgg caggccgcag
     3721 gccagggcct cggccaccga gacaccccag cgctcctcgc gcgccgggtg gatgaagacg
     3781 tcgg
//
LOCUS       DASPJI010000131         4985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_470320, whole genome shotgun sequence.
ACCESSION   DASPJI010000131 DASPJI010000000
VERSION     DASPJI010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470320"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2153)
                     /locus_tag="VEL74_03640"
     CDS             complement(<1..2153)
                     /locus_tag="VEL74_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61649.1"
                     /translation="MLYFDTSLELEGLTLFRDYNDPKRFYYMPRAPHLTVENGAPMFQ
                     LLIYRRDITDNPDFREGDRTGGGFLTMTVDLSVPLGVIESVRGELGSRAGGDVELVPV
                     PFEDGSVRVSALGFSAGRARALEEAGGAGSAEPVPGQAEAGPRFVERILGSARPSLYG
                     DNRTVFTIEMSAEGAQLMRASLQDPGATQVAVIYDLEYRGLLPAYEGKITIDFKQSYS
                     YLRSRFALNTLVFKADIDAEMERLRKEGHIKIEEVDYLQSDPAKLAERAQKLQELAKE
                     LATWGFFRPGLTPGKVLADDRGNITVYDATEAAARSEAGFTAPLAAAGTGRGSAGDVS
                     GPRQQGDSANPSTTRVGGQAAPPQGAAQPAGTGATQPAAGGGGAVEAWNRAGRPQAAF
                     LLKSLSQEEQQLITYDLRQIAAAKRSAAPQGSIRMLPGAAQLAGRIKEVDLNDPFFER
                     IVGAVSTTADLAAAGVNSMVVKLRYGVRDDGTAPKDTQEFPLSKAGDKGSYAFFMDRR
                     RSVELEYQVVASYKAGFAIGSTDTQATSPWIRTTTRNLDVDPRIVGAVFPVSLVLGNV
                     DWTSVQSVQSTVLYDHGAVHAERTVVLTQAAPAAVVPVRPPEAGSRRFRVRSVFFYGN
                     VQEVVEVEGEGDSLVVINPPTSRAVPVSLSATDPLGRFRKVTVELSYGGPGQPEQIRL
                     VELPGDGSTQSWTFFRPSDQDEARYRYRVTLFGKDG"
     gene            2288..4285
                     /locus_tag="VEL74_03645"
     CDS             2288..4285
                     /locus_tag="VEL74_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61650.1"
                     /translation="MLTLADRLRIEPFTLFRDVIYKDGARSYTARLYALPDAPRLARD
                     QDGGPAFGFVWYRTPAGEEAPGGGGMVTLTVELGPSSEEKERLAAAVAAALPAERLED
                     IEILSMPFRAGTVSLAFAGEEGAREGPTGDFAARVAGSGPARLAGSQRATFTVELTRD
                     GAALLHQCLERGLDLFHARYDLLYDYRLDDVELRIWCDARGEDRLSGVEVVTTRHLDP
                     EEHQALERIGHELLDAALAGGDTVVDLSFTQAFPVEGRAALDGTIRLGVPLAELANSG
                     QRVRQVDLEGSFFTVLEVPVHCTVDFSEGPIETVRVKVEYDVTGPAGRVHTTGEYLFS
                     RAASGPYLFRTALASPGQRTVRCEAEVFYAGNAEPLRVAYPPAEGTAIVLDLDRLGVL
                     RVEVGLRDVPLERIRAAVVDLEIPPLGLTHRMVLDGETPAGLWQPVTRVSTPPPFRYR
                     ATWLTAGGERIETEWRDSTAGAIALDAPRSLVSKARVQLVGAGDFSGLAQIVVDLEAE
                     VEGRVHRGQLAFSTAGESRTWEVPGAQLGFRYRSRFTLVGTDGAVRSLEWVDQDGPVL
                     VVRDPLRFEVRIVSRLLDLGGSLNLALLALEPEDAEGAGRSTLILRRPDEEAVWSFGL
                     AAPDRHRYRWQLTLIPKQGERTTTPWQEAEAGVLVLRPPAQ"
     gene            4296..>4985
                     /locus_tag="VEL74_03650"
     CDS             4296..>4985
                     /locus_tag="VEL74_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61651.1"
                     /translation="MLTFESPFYEIAGIVIFRDHAVPTLFHYLAGPPRLARDEQGRPH
                     MLLLKYRHALDSMGGADTVAREQLGGGFLLLEVECGLARDPRRDDDVRNEIVQKLAST
                     TSVDSGKISLVPVLYTEGKVNVLALDAQQPAEGTPAGDAEAHKRFVRGIQGTSTPSLL
                     DDQRAIFSISLTPDAVTLIEEAYRSELSPIGVMYELEFAGLRPALAVKAHVEKKKVYE
                     FFKAGLHLGVHS"
BASE COUNT          674 a         1747 c         1797 g          767 t
ORIGIN      
        1 ccgtccttgc cgaacagggt gacgcggtac cggtaccgcg cctcgtcctg gtcgctggga
       61 cggaagaagg tccacgattg ggtggagccg tcgccgggca gctcgaccag ccggatctgc
      121 tcgggctggc cgggcccgcc gtaggacagc tcgacggtga ccttgcggaa gcggcccagg
      181 ggatcggtgg cgctgaggct caccggcacc gcccgtgacg tcggtggatt gatgactacc
      241 agggagtccc cctcgccctc gacctccacc acctcctgga cgttgccgta gaagaagacg
      301 ctgcgcacgc ggaagcggcg gctccccgcc tccggcggcc ggaccggcac caccgccgcc
      361 ggcgccgcct gggtcagcac cacggtgcgc tcggcgtgga cagcgccatg gtcgtagagg
      421 acggtgctct gcacgctctg cacgctggtc cagtcgacgt tgccgaggac caggctcacc
      481 gggaagacgg cgcccacgat ccgcgggtcg acgtccaggt tgcgggtggt cgtccggatc
      541 cagggcgacg tcgcctgggt gtcggtggag ccgatggcga agcccgcctt ataggaggcc
      601 accacctggt actcgagctc gacgctccgc cggcggtcca tgaagaacgc gtagctcccc
      661 ttgtcgccgg ccttggagag ggggaactcc tgggtgtcct tgggagcggt gccgtcgtcg
      721 cgcacgccgt agcggagctt gaccaccatc gagttgactc cggccgcggc caggtcggcg
      781 gtggtggaga cggcgccgac gatgcgctcg aagaacggat cgttgaggtc gacctccttg
      841 atgcgtccgg cgagctgcgc ggcgcccggc agcatgcgga tggagccctg gggcgcggcc
      901 gaccgcttgg ccgcggcgat ctggcggaga tcgtaggtga tgagctgctg ctcctcctgg
      961 ctgagcgact tcaggaggaa agcggcctgc ggccggccgg cgcggttcca ggcctcgacg
     1021 gcccctcccc cgccggcggc aggttgcgtc gcgccagtgc ccgcgggctg agcggcgccc
     1081 tgcggcgggg ccgcctggcc gccgacgcgg gtggtgctgg ggttggccga gtcaccctgc
     1141 tggcgggggc ccgatacgtc gcccgccgag ccgcgcccgg tgccggcggc ggccaggggg
     1201 gcggtgaagc ccgcctcgct gcgcgccgcg gcctcggtgg cgtcgtagac cgtaatgttg
     1261 ccccggtcgt cggcgagcac cttgccgggc gtgaggccgg gccggaagaa gccccaggtg
     1321 gcgagctcct tggccagctc ctggagcttc tgcgcccgct cggccaactt cgccgggtcg
     1381 gactgcaggt agtccacctc ctcgatcttg atgtgcccct ctttgcgcag ccgctccatc
     1441 tcggcgtcga tgtcggcctt gaacaccagg gtgttcaggg cgaagcggct gcgcaggtag
     1501 gagtagctct gcttgaagtc gatggtgatc ttgccttcgt aggcgggcag gaggccgcgg
     1561 tactcgaggt catagatcac ggccacctgg gtggcgccgg ggtcctgcag ggaggcgcgc
     1621 atgagctgcg ccccctcggc gctcatctcg atggtgaaga ccgtccggtt gtcgccgtag
     1681 aggctgggcc gggccgatcc caggatgcgt tcgacgaagc gcgggccggc ctcggcctgt
     1741 cccggcaccg gctcggcgct accggcgccg ccggcctctt cgagcgcccg ggcgcggccg
     1801 gcggagaagc cgagggcgct cacccgcacg ctgccgtcct cgaacggcac cggcaccagc
     1861 tcgacgtcgc cgccggcgcg gctgccgagc tcgccgcgca cgctttcgat gacacccagg
     1921 ggcacactca ggtcgacggt catggtgagg aagccgccgc cggtacggtc cccctcccgg
     1981 aaatcggggt tgtcggtgat gtcccggcga tagatcagga gctggaacat cggcgcgccg
     2041 ttctccaccg tcaggtgggg ggcgcggggc atgtaataga agcgtttcgg atcgttgtag
     2101 tcgcggaaga gggttaggcc ttccagctcg agtgaggtgt cgaagtagag catggccgct
     2161 cctggtcggg attcgcgtcg aagctcgatg gcgtgaggtc aggatgcaac gtttctcccg
     2221 gtccgcgcgc cgaattcgtc gtcggctgtt gaggcgcggg atcgagagga aacgttacgg
     2281 aggattcatg ctgactctgg cggaccggtt gcggatcgag cccttcaccc tgttccggga
     2341 cgtgatctac aaggatggcg cccggagcta tacggcgagg ctctatgccc tgccggacgc
     2401 cccccgcctg gcgcgggacc aggacggcgg cccggcgttc ggcttcgtct ggtaccgcac
     2461 gccggccggc gaagaggcgc cgggaggggg cgggatggtc accctcaccg tggagctggg
     2521 gccatcttct gaagagaagg agcggctcgc cgcggcggtg gcggcggcgc tgccggcgga
     2581 acggctggag gacatcgaga tcctctcgat gccgttccgg gcgggcacgg tctccctcgc
     2641 cttcgccggc gaggaagggg cccgggaggg gccaaccggc gacttcgccg cgcgggtcgc
     2701 cggcagcggc ccggcccggc tcgccggctc ccagcgtgcc acgttcaccg tcgagctgac
     2761 ccgggacggc gccgccctcc tccaccagtg cctcgagcgg gggctcgacc tcttccatgc
     2821 ccgctacgac ctcctctacg actaccggct ggacgacgtg gagctgcgga tctggtgtga
     2881 tgccaggggg gaggaccggc tgtccggtgt cgaggtcgtc accacgcgcc atctggaccc
     2941 ggaggagcac caggcgctcg agaggatcgg ccacgagctg ctcgatgcgg cgctcgccgg
     3001 cggcgacacg gtggtcgacc tctccttcac ccaggccttc ccggttgaag ggcgggccgc
     3061 gctcgacggc acgatccggc tcggtgtccc gctggcggag ctggccaatt ccggacaacg
     3121 ggtgcggcag gtggatctcg aagggagctt cttcacggtg ctggaggtcc ccgtccactg
     3181 cacggtggac ttctccgagg gtccgatcga gaccgtccgg gtaaaggtgg agtacgacgt
     3241 caccgggccg gcgggccggg tccacaccac cggcgagtac ctcttcagcc gcgcggcctc
     3301 agggccctat ctcttccgga ccgccctggc gtcgcccggc cagcggaccg tgcgctgcga
     3361 ggccgaggtc ttctatgccg gtaacgccga gcccctgcgc gtcgcctatc cccctgccga
     3421 gggcaccgcg atcgtgctcg acctcgaccg tctcggagtg ctgcgggtgg aggtcgggct
     3481 gcgtgacgtg cccctggaaa ggatccgcgc cgccgtggtc gatctcgaaa tcccccctct
     3541 ggggctcacc catcgcatgg tcctggacgg cgagacgccc gccggcctct ggcagccggt
     3601 cacccgggtt tccactcccc cgccgttccg gtaccgggcg acctggctga ccgccggcgg
     3661 cgagcgcatc gagaccgaat ggcgcgactc cacggccggc gccatcgccc tcgacgcacc
     3721 ccggtcgctg gtctccaagg cccgggtgca gctcgtcggc gcgggcgact tttccggcct
     3781 ggcgcagatc gtggtggacc tggaggccga ggtggaaggc cgggtccatc gcgggcagct
     3841 cgccttctcc accgccgggg agagccggac ttgggaggta ccgggggccc aactcgggtt
     3901 ccggtaccgg tcccgcttca ccctggtcgg caccgatggc gccgtccgct ccctggagtg
     3961 ggtggaccag gacggtccgg tgctggtggt gcgcgacccg ctccgcttcg aggtgcggat
     4021 cgtctcgcgc ctgctcgacc tcggcggctc cctgaacctg gccctgctcg ccctggagcc
     4081 cgaggacgcg gagggcgccg gacgctccac cctgatcctg cggcgccccg acgaggaggc
     4141 ggtctggagc ttcggcctcg ccgccccgga ccgccaccgc taccgctggc agctcacgtt
     4201 gatccccaaa cagggggagc ggaccaccac gccctggcag gaagccgagg ccggcgtgct
     4261 ggtgctccgt ccacccgcgc agtgaggacg tccccatgct gaccttcgaa tcgcccttct
     4321 atgagatcgc gggcatcgtc atcttccgcg accacgccgt tcccaccctg ttccactacc
     4381 tggccgggcc gccccgtctg gcgcgggacg agcagggccg gccccacatg ctgctcctca
     4441 agtaccggca cgccctggac tccatgggcg gcgccgacac cgtggcccgc gagcagctcg
     4501 gcggcggctt cctcctcctg gaggtggagt gcgggctggc ccgcgacccc cgacgggacg
     4561 acgacgtccg gaacgagatc gtccagaagc tggcgtcgac gacctccgtc gactccggca
     4621 agatctccct ggtgccggtg ctctacaccg agggcaaggt caacgtcctg gccctggacg
     4681 cccagcagcc ggccgagggg acgcccgcgg gggacgccga ggcccacaaa cgcttcgtcc
     4741 gcggcatcca gggcacctcg accccctcgc tgctcgacga ccagcgcgcc atcttctcca
     4801 tctccctgac gccggacgcg gtcaccctga tcgaggaggc ctatcgctcc gagctgtcgc
     4861 ccatcggcgt catgtacgag ctggagttcg cgggcctgcg gccggcgctg gcggtcaaag
     4921 cccacgtgga aaagaagaag gtctacgagt tcttcaaggc gggcctccac ctgggagtgc
     4981 actcg
//
LOCUS       DASPJI010000132         4078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_636434, whole genome shotgun sequence.
ACCESSION   DASPJI010000132 DASPJI010000000
VERSION     DASPJI010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636434"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4078
                     /locus_tag="VEL74_03655"
     CDS             <1..>4078
                     /locus_tag="VEL74_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61652.1"
                     /translation="RAVALARERGLDTELADLFQHQSVAELGLAVRLVRLVEGDTEGT
                     RSEPLSLLSAEDRRKLPPGIEDAYPLAMLQAGMLFHMGLTPEEPQYHNVDSWHVRGHF
                     EVEAFQEAVRRVVARHPLLRTSFAMTGYSEPLQLVHAAAELPLTVEDIRHLAPAAQEA
                     AIDRLILQEKRRPFDLTRAPQLRFHVFLRSAEAFQFTLGENHAIFDGWSLHATLSEIF
                     ELYFALLSGAAPEPLPPLALTYREHVRREREALASLAAEEHWRRLLQGAELVELPSWP
                     PGWCRPGQYRMERLNLPVPPAVTAGLRRLALEVAVPLKSVLLAAHLKVLSLLAGRPDV
                     VSGVVLNGRSEESEGDQVRGLFLNTLPLRLRLPDGSWADLVRAAFQAELELLPHRRYP
                     FAAVQRRWGDRPLFEIIFNYIRFHVVQDLMRSGNVEVLQFKQAEGGHFKLQAHFNQDL
                     AQTSVGLGLEYDSHVVPSALVREIGDLYLRTLESMAGAADGEHGEVSILRTTAGQKLL
                     REWNDTDAELAGARHCLHELIEEQARRTPERVAVAIEGESLTYQQLSRRSNQLARSLR
                     HLGVGPEVRVALCAGRSPEMVVALLGILKAGGAYVPLDPAHPAERLALVLGDSAPEVL
                     VTEERWLERLGNGGRAVLPRVVCLDRDRGRVAAEERAGLEAAPDGGPGSLAYVIYTSG
                     STGRPKGVCLPHGAVVNFLLAMAQRLGLGGNEVIPALTTLTFDIAGLEVYLPLLLGGR
                     VEVVGTEEAADGRQLVARLAAAGMTAMQATPATWRLLLDAGWSEQPGLAALCGGEALP
                     RPLASDLLERGVELWNVYGPTETAIWSAAGVVAPAGEGAAVGLGRPLANTRFYVVGPG
                     LEPVPAGAAGELLIAGAGVARGYWGRPALTAERFVPDAWSPDGGARLYRTGDLVRHRL
                     DGDLEFLGRADHQIKLRGFRIELGEIEAALLEHPGVSLAAVVPVGEGTDRRLVACFVS
                     PAGQAPAEAELRERLRGLLPAYMVPAAFVLLPEMPLTPSGKIDRRVLTQSAMEVQVSG
                     APRRAPRAPIEEIVAGLWAEVLGVPRVGLDDDFFQLGGHSLMATRLFSRLRETLHVEL
                     PLRTLFEAPRLADLAARVQAALAVGGGVLAPPLERVSRDLPLPLSFAQEGLWFIDRLH
                     PGGTAYNIPLTVRLTGQLDAATLSRTLAEIVRRHETLRTVFAVAGDRPVQVILPEPLA
                     ALATVDLAGLPPDQREREAGRLATEQMRWPFDLARGPLLRHELLRLGQESHLLVMGCH
                     HIIADAWSIDVLARELAALYPALHAGRPSPLPELPVQYADFAAWQRGWLQGETLRVLL
                     DHWRQVLAGAPFLELPIDRPRRAGQGFRGGELRATLPAA"
BASE COUNT          489 a         1380 c         1586 g          623 t
ORIGIN      
        1 gcgggccgtg gcgctggccc gggagcgggg gctcgatacg gagctggcgg acctgttcca
       61 gcaccagagc gtcgccgagc tggggttggc ggtccgcctc gtccgtctcg tcgagggcga
      121 cacggagggg actcggagcg agccgctgag cctgctctcg gcggaggacc gccgcaagct
      181 gccgccagga atcgaggacg cctatccgct ggccatgctc caggcgggga tgctgttcca
      241 catgggactg accccggagg agccgcagta ccacaacgtg gacagttggc acgtgcgtgg
      301 gcatttcgag gtggaggcgt tccaggaggc cgtgcgccgg gtggtggcgc gccatccgct
      361 gctgcgcacc tccttcgcca tgaccggcta cagcgagccg ctgcaactgg tgcacgcggc
      421 cgccgagctg ccgctcacgg tggaggacat ccggcatctg gcgcccgcgg cgcaggaggc
      481 cgccatcgat cgcctgatcc tccaggaaaa gcggcggccg ttcgatctga cgcgggcgcc
      541 gcagctccgc ttccatgtct tcctgcgctc ggccgaggcc ttccagttca ccctggggga
      601 gaaccacgcc atcttcgacg gctggagcct gcacgcgacg ctgtcggaga tcttcgagct
      661 gtatttcgcc ttgctgtcgg gagcggcgcc cgagccgctg ccgccgctgg ccctcaccta
      721 ccgcgagcac gtgcggcggg agcgggaggc gctcgcctcg ctggcggcgg aagagcactg
      781 gcggcgcctg ctgcagggcg cggagctggt cgagctgccg tcctggccac cgggatggtg
      841 ccggcccggc cagtaccgga tggagcggct gaacctgccg gtgccccccg cggtgacggc
      901 gggcctcagg cgcctggcgc tggaggtggc ggtgccgctc aagagcgtgc tgctcgctgc
      961 ccacctcaag gtcctgagcc tgctggcggg acggccggac gtggtgagcg gcgtggtctt
     1021 gaacggcagg tcggaggaga gcgaggggga ccaggtgcgc ggcctcttcc tcaacacgct
     1081 gcctctgcgg ctccgtctgc cggacggctc gtgggccgac ctcgtgcgcg ccgccttcca
     1141 ggccgagctg gagctgctgc cccaccgccg ctatcccttc gccgccgtgc agcgccggtg
     1201 gggggaccgt cccctgttcg agatcatctt caactacatc cgcttccacg tggttcagga
     1261 cctgatgcgc tccgggaacg tggaggtcct gcagttcaag caggccgaag gggggcactt
     1321 caagctccag gcccacttca accaggatct ggcgcagacg agcgtcgggc tgggcctgga
     1381 gtacgactcg cacgtggtgc cgtcggcgct ggtgcgggag atcggcgacc tctatctccg
     1441 cacgctggag agcatggccg gcgccgcgga cggtgagcat ggggaggtgt cgatcctgcg
     1501 cacgaccgcc gggcagaagc tgctgcggga gtggaacgac acggacgcgg agctggcggg
     1561 ggcgcggcac tgtctccatg agctgatcga ggagcaggcg cggcggactc cggagcgagt
     1621 ggcggtggcg atcgagggcg agagcctgac ctaccagcag ctttctcggc gttccaacca
     1681 gctcgcgcgc tctctccgcc acctgggggt ggggccggag gtgagggtgg cgctctgtgc
     1741 cggcaggtcg ccggagatgg tggtggcgct cctcggcatc ctcaaggcgg gaggagccta
     1801 tgtgccgctc gatccggcgc acccggcgga gcggctggcg ctggtgctcg gcgacagcgc
     1861 cccggaggtg ctggtcaccg aagagcgctg gctggagcgg ctggggaacg gcggccgcgc
     1921 cgtgttgccg cgcgtggtct gcctggaccg cgaccgcgga cgggtcgccg cggaggagag
     1981 ggccgggctg gaggcggcgc cggacggagg tccggggagc ttggcgtacg tgatctacac
     2041 ctcgggctcc acgggccggc cgaagggcgt ctgcctgccg catggagccg tggtcaattt
     2101 cctcctcgcg atggcgcagc ggctgggtct gggcggaaac gaggtgatcc cggcgctgac
     2161 cacgttgacc ttcgacatcg ccgggctgga ggtctatctg ccgctgctgc tgggcggccg
     2221 ggtggaggtc gtcgggactg aggaggccgc cgatgggcgc cagctcgtgg cgcggctcgc
     2281 ggcagcgggg atgacggcga tgcaggcgac gccggcgacc tggcgtctgc tgctcgacgc
     2341 gggctggagc gaacagccgg gcctcgcggc cctgtgcggc ggcgaggcgc tgccgcggcc
     2401 tttggcgtcg gacctgttgg agcgcggtgt cgagctgtgg aacgtgtacg gccccacgga
     2461 gacggcgatc tggtcggccg ccggggtggt ggcgccggcg ggggagggcg ccgccgtggg
     2521 cctcggccgg ccgctcgcca acacgcggtt ctacgtcgtg gggcccgggc tcgagccggt
     2581 gccggccgga gccgccgggg agctgctgat cgccggcgcc ggggtggcgc gtggctattg
     2641 gggccggccg gcgctgacgg cggagcgctt cgtgcccgac gcctggagtc cggatggcgg
     2701 cgcgcggctc taccggaccg gagacctcgt gcgccatcgc ctggatgggg atctggagtt
     2761 cctcggccgc gccgaccacc agatcaagtt gcggggcttc cgcatcgagc tcggcgagat
     2821 cgaagccgcg ctgctggagc acccgggcgt cagcctggcc gcggtggtgc cggtgggtga
     2881 ggggaccgac cggcggctgg tggcctgctt tgtgagcccc gccgggcagg cgccggccga
     2941 ggccgagctc cgcgagcggc tgcgcgggct gctgccggcc tacatggtgc cggcggcgtt
     3001 cgtgctcctg ccggagatgc cgctgacgcc gagcggcaag atcgaccgcc gggtgctgac
     3061 gcaatccgcc atggaggtcc aggtgtccgg ggcgccgcgg cgtgcgccgc gcgcccccat
     3121 cgaggagatc gtggccgggc tctgggcgga ggtgctcggg gtgccgcggg ttgggctgga
     3181 tgacgacttc ttccagctcg gcggtcactc gttgatggcc acccggctgt tctcccggct
     3241 gcgcgagacc ctccacgtcg agctgccgct gcgcaccctc ttcgaggctc cccggctggc
     3301 cgacctggcg gcgagggtgc aggccgctct ggctgtcggt ggcggcgtgc tcgcgccgcc
     3361 cctggagcgg gtttcccgcg acctgccgtt gccgctctcg tttgctcagg agggcctctg
     3421 gttcatcgac cgcctccatc cgggcgggac ggcctacaac atccccctga cggtgcgcct
     3481 caccggccag ctcgacgcgg ccaccctgag ccgcaccttg gcggagatcg tccggcgtca
     3541 tgagaccctg cgcacggtct tcgcggtggc cggggaccgg ccggtgcagg tgatcctgcc
     3601 cgagccgttg gcggccctcg cgacggtcga cctggccggc ctcccccctg accagcggga
     3661 gcgcgaagcg ggccgcctgg ccaccgagca gatgaggtgg cccttcgatc tggcgcgcgg
     3721 tcccctgctt cggcatgagc tgctccgtct tggccaggaa tcgcacctgc tggtgatggg
     3781 ctgccatcac atcatcgccg acgcctggtc catcgacgtg ctggcccgcg aactcgcggc
     3841 gctctacccg gcgctccacg ccggccggcc cagccctctg cccgagctgc cggtgcagta
     3901 cgcggacttc gcggcctggc agcgcggctg gctgcagggt gagacgttgc gggtgctgct
     3961 tgaccactgg cggcaggtgc tcgccggggc acccttcctt gagctcccca tcgacagacc
     4021 gcggcgtgcg gggcagggat tccgcggcgg cgagttgcgc gcgacgttgc cggccgcc
//
LOCUS       DASPJI010000133         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_753334, whole genome shotgun sequence.
ACCESSION   DASPJI010000133 DASPJI010000000
VERSION     DASPJI010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753334"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VEL74_03660"
     CDS             complement(<1..482)
                     /locus_tag="VEL74_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HYG61653.1"
                     /translation="MAAGYPGTGDLIRRMVEASEDPIDPDLPFFQIADRFVASQGAGD
                     LADLLQRLLGPPRPPAPLHKAIARLAKAGFFQAIVTTNYDNLLERALDDAGVKPIVQH
                     LELNATVVADGHIRLLKLHGSREDWLSVVLSGESYEKFGRQYPFLVSQLDVLLRQHPV
                     L"
     gene            1229..2785
                     /gene="purH"
                     /locus_tag="VEL74_03665"
     CDS             1229..2785
                     /gene="purH"
                     /locus_tag="VEL74_03665"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HYG61654.1"
                     /translation="MMPARRALISVFDKRGLDDLARGLARLGVEIVSTGGTLKALQSL
                     GVPVVGVSDVTGFPEILDGRVKTLHPRIHGGILARRDAGDHAAQLAEHGIDPIDLVVV
                     NLYPFRETLAAGASFDETVEMIDIGGPAMVRAAAKNHHGVAVVVDPADYAEVLAALEA
                     NGRTVPEPLRRRLAAKAFRHTHEYDGAVASWLETQSAPAGEAVALFPPGFSLGLSRVM
                     ELRYGENPHQGGAVYAVDGAGGALGGFRQIQGKELSWNNLLDADAARKMAALFDEPTV
                     VIVKHNNPCGIGRGTDLVEAYQRALATDPVSAFGSIIATNRPVDGALAEALADLFVEV
                     LLAPAFDGGALERFGAKKNLRLLECPAYAPSSREIELRAIAGGFLAQPPDAFPDDPAG
                     WTCQTRRQPSPEERRALELAWKVMRYVKSNAIVVANEHQTVGIGAGQMSRVDSCNLAI
                     QKAQLPVAGTVAASDAFFPFRDGLDVLARAGITAVVQPGGSKRDDEIIAAADEHGMAM
                     LFTGVRHFRH"
     gene            complement(2817..>3250)
                     /locus_tag="VEL74_03670"
     CDS             complement(2817..>3250)
                     /locus_tag="VEL74_03670"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HYG61655.1"
                     /translation="LRGGPRQVVKLSRPPGEEAAGAEALPPPPENWESWRDGARACGL
                     GFSDLERIAKVVRSAGRPAVEVGPGADRPAALGLRQRLAAGGERVVVIPTAKKGKPAC
                     SVWIGPLASPQVAQEMAQRVSAAYGVDARAGYLPDVEKLGE"
BASE COUNT          495 a         1143 c         1178 g          434 t
ORIGIN      
        1 aggacggggt gctggcggag gaggacgtcg agctgggaaa ccaggaaggg gtactgccgg
       61 ccgaacttct cgtaggactc ccccgagagg acgacgctca gccagtcttc ccgggagccg
      121 tggagcttca gcagacggat gtgcccatcc gctaccaccg tggcgttcag ttccaggtgc
      181 tgaacgatcg gcttcacccc ggcgtcgtcg agcgcccgct ccaggaggtt gtcgtagttc
      241 gtggtgacga tcgcctggaa gaaccccgcc ttggcgagcc gggcgatggc cttatggagc
      301 ggcgcgggcg ggcgcggggg tccgaggagc cgctggagga ggtccgcgag gtcgcccgcg
      361 ccctggctgg cgacgaagcg gtccgcgatc tggaagaacg ggagatccgg atcgatggga
      421 tcctcggatg cctcgaccat ccgccggatc agatcgcccg tcccgggata gcccgcggcc
      481 atcgaagccc ccgctccgac ccagaggacg ggacgcttgt ccttcaggaa cctggcgagg
      541 ctgtcccgat ccgccttgag aggcggccgg ggggaggatc cggatggcgg agtggtcacg
      601 tcattaagga tagcagtgga gaccatgggc cggagggagg gttcagaccg ccgaaccgag
      661 gaagcgaacc gcgggatcga gggaacggac ttccggcggg tcgtctcatc gggccggacg
      721 gaggcctcgc cggtccgaac ggatggaacg atgggccggt gggtcggcat gtccggccgg
      781 cagaggaacc gagccaccgg acgggtgaag catcccggcg gcgggatgaa acggccagcc
      841 ggttcgagga accgctgagc cgggcggagg aaacgaaccg ccggacgtag gagacgagcc
      901 gccgccgggg tgttacctcg gtccgaaggg aggacacacc caaggggcgg atgaactgga
      961 ccgccggatg ggtgaactgg ccttcccgaa ggtcgttatc ttggggcgga cgggtgaatg
     1021 gtggggccgg cgggatgaga cgcggactcg gagggatgag atgcggcttc acttgacgtt
     1081 ggagccgcta gagagtctag atgtcgggga gcgggtcggg gagacgggag agggggcccg
     1141 gggggtgagg gccccgctgc tatagttccc ccgccggcgc cctgccgcgc cggttcagcc
     1201 cgatcatgaa cttttcgagg agccgaccat gatgccggca cgccgtgctc tgatatccgt
     1261 gttcgacaag cggggcctgg acgatctcgc ccgcggcctc gcccgcctgg gggtcgagat
     1321 cgtctccacc ggtggcaccc tcaaggccct tcagagcctg ggcgtgcccg tcgtcggcgt
     1381 ctccgacgtc accggcttcc ccgagatcct cgacggccgg gtgaagaccc tccatccgcg
     1441 catccacggc ggcattctcg cccgccgcga cgccggcgac cacgccgccc agctcgcgga
     1501 gcacggcatc gaccccatcg acctggtggt ggtcaacctc tatccgttcc gcgagaccct
     1561 cgccgccggc gcctcgttcg acgagacggt ggagatgatc gacatcggcg gcccggcgat
     1621 ggtccgcgcc gcggccaaga accaccatgg cgtcgccgtg gtggtcgatc ccgccgacta
     1681 cgccgaggtg ctcgccgccc tggaggcgaa cggccgcacc gtccccgagc cgctgcgccg
     1741 gcgcctcgcc gccaaggcgt tccgccacac ccatgagtac gacggcgccg tcgcctcgtg
     1801 gctggagacg cagagcgcgc cggccggcga agcggtcgcc ctcttcccgc cgggcttctc
     1861 gctcggcctg tcgcgggtca tggagctgcg ctacggcgag aacccgcacc agggcggggc
     1921 ggtctacgcg gtggacggcg ccggcggcgc gctcggcggg ttccgccaga tccagggcaa
     1981 ggagctgtcg tggaacaacc tgctcgacgc cgacgcggcg cgcaagatgg cggcgctgtt
     2041 cgacgagccc acggtggtca tcgtcaagca caacaacccg tgcggcatcg gacgcggaac
     2101 ggacctcgtc gaggcctacc agagggcgct cgccaccgac ccggtatccg ccttcggctc
     2161 gatcatcgcc accaaccggc cggtggacgg cgcgctcgcc gaggcgctgg ccgacctctt
     2221 cgtcgaggtg ctcctcgccc ccgccttcga cggcggcgcc ctggagcgct tcggcgccaa
     2281 gaagaacctg cggctcctgg agtgcccggc ctacgcgccg tcctcgcgcg agatcgagct
     2341 gcgcgccatc gccggcggct tcctcgccca gccgccggac gccttcccgg acgaccccgc
     2401 cggctggacc tgccagacgc gccgccagcc gtcgccggag gagcgccgcg ccctggagct
     2461 ggcctggaag gtgatgcgct acgtgaagtc caacgccatc gtcgtcgcca acgagcacca
     2521 gacggtgggg atcggcgccg gccagatgag ccgggtggac tcctgcaacc tcgccatcca
     2581 gaaggcccag ctccccgtcg ccggcaccgt cgccgcctcg gacgccttct tccccttccg
     2641 cgacggcctc gacgtcctgg cccgcgccgg catcactgcc gtcgtccagc cgggcggctc
     2701 caagcgcgac gacgagatca tcgccgctgc cgacgagcac gggatggcga tgctgttcac
     2761 gggggtgcgg cacttcaggc actgagcccc ctcgccggga gagggggaag cggatctcac
     2821 tctcccagct tctcgacgtc cggaaggtag cccgcccggg cgtccacgcc gtaggcggcg
     2881 gagacccgct gggccatctc ctgcgccacc tggggcgatg ccagaggtcc gatccagacc
     2941 gagcaggcag gctttccctt cttggccgta gggatcacga ccacccgctc gcctccggcg
     3001 gcgagacgct ggcgcaggcc gagagccgcc ggacggtccg ccccgggccc gacctcgacc
     3061 gccggacggc cggccgagcg caccaccttg gcgatccgct ccaggtcgga gaagccgagc
     3121 ccgcaggcgc gggcgccgtc gcgccaggac tcccaattct cggggggcgg cggcagggcc
     3181 tcggcgccgg cggcctcctc cccgggcggc cgggagagct tcaccacctg gcgagggccg
     3241 ccgcgaagcg
//
LOCUS       DASPJI010000134         6939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_810074, whole genome shotgun sequence.
ACCESSION   DASPJI010000134 DASPJI010000000
VERSION     DASPJI010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6939)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810074"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /gene="glgP"
                     /locus_tag="VEL74_03675"
     CDS             complement(<1..1156)
                     /gene="glgP"
                     /locus_tag="VEL74_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HYG61656.1"
                     /translation="MTDPSCDATLRKLRELAQNLWWSWQPDIRTLFKTLDPVLWRLVY
                     HNPVAMLQRLSDEEIARRVADLEIQTRIDQAHRRLVEYLRADEGWGLTHAGPLQARPV
                     AYFSAEFGLHQSLPIYSGGLGVLAGDHLKSMSDLGVRVVGVGLLYHQGYVHQLIDENG
                     WQQDLYEPIAAAELPVKPVPALSGENGGGDSDGDSEGGVRQAFVDVELPGRNVRLRIW
                     RVHVGRNDLLLLDARDEANTPEDQELTARLYGGDQETRIQQEILLGVGGFRALGALGI
                     EPSVLHLNEGHSAFALLERARHMVQHQGLSATDAIREVAATSAFTTHTPVEAGHDRFR
                     PDLAARHLEPLAAGLGMPMDEVLDLGSERPGDRGAPFCPTVLALNLSRRAN"
     gene            complement(1333..1587)
                     /locus_tag="VEL74_03680"
     CDS             complement(1333..1587)
                     /locus_tag="VEL74_03680"
                     /inference="COORDINATES: protein motif:HMM:NF021471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HYG61657.1"
                     /translation="MAKATAEKTERKVYHVVPNSSAEKWLVTQENGDYRTEHDTKEEA
                     VQEARKLAQKGPLGQIKVHKKDGNMEYESTYGEDPPESPS"
     gene            1677..3818
                     /locus_tag="VEL74_03685"
     CDS             1677..3818
                     /locus_tag="VEL74_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYG61658.1"
                     /translation="MKAHTPRLTRSSFLAVLLLSLSLVYPAAAPAQEAKGGFTLEQVL
                     SAPFPSDLTAAGTGGQVAWVQNARGVRNLWIAAPPDYRGRQVTSYTRDDGQALSGLAW
                     SADGKTLFYVRGSGANRQGEVPNPAGDPGGAEQAVWRISAEGGEPVRIGVGSQPTPSP
                     KGDGVAFVRRGQIFWAPLASDGAEGGKSAEPVQWLQARGTASALRFSPDGSKLAFTSG
                     RGDHNFIGVYDVAAKAVRWMAPGVDQDGSPAWSPDGTRLAFVRVPAQRRTRLFLSNRS
                     GHPWSILVADPATGQARTVWRARPGDGSVFRVVVADDQLLWGAGDRLVFPSEADGWLH
                     LYSVPVAGGEATLLTPGEFEVEYVTLSPDRRTVLYNSNQGDIDRRHLWRVDVAGGTPA
                     ALTRGDGIEWSPTAVGNSLAYLRSGARRQAHAVIQVGNGEPRELAPGTVPAEFPEAAL
                     VEPEPVVFTSGDGMRIHAQLFKPRDIRQGERRPALLFLHGGSHRQMLLGWHYSGYYNN
                     TYAMNQYLAGRGYLVLAVNFRAGTGYGMHFREALRQGAGGASEFADVLGAALYLRGRP
                     DVDPRRIGLWGGSYGGYLTAMGLARAPELFAAGVDIHGAHDWNDEILNFVPSYEPTPE
                     EERLAFQSSPMAWLDLWRAPVLLIHGDDDRNVPFSESVRLAEALRERGVEVEAVAIPD
                     EIHGFLRYQSWLDVFRAAGDFFDRKLKAKTD"
     gene            3840..4328
                     /locus_tag="VEL74_03690"
     CDS             3840..4328
                     /locus_tag="VEL74_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HYG61659.1"
                     /translation="MEGMAGLLWVLAVILVVVGVAGTVLPALPGAPLVFAGLVVAAWA
                     EGFEKVGWITLTLIGLLTLLTFAVDFLASTLGAKRVGASWMAVVGATLGIVAGFFLGL
                     PGLLLGPFVGAFLGEYLARRDWAQARRAGIGTWIGFLLGTVLKLTLIFAMIGLFAAAY
                     FF"
     gene            complement(4331..5047)
                     /locus_tag="VEL74_03695"
     CDS             complement(4331..5047)
                     /locus_tag="VEL74_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HYG61660.1"
                     /translation="MCGRYTLTSSGEQVATLFDLMEMPDLQARYNMAPTQDAPVVRML
                     EPGGPRVLSYLRWGLVPYWADDPAIGNRMINARSESVAEKPAYRTSFKKKRCLVVTDG
                     FYEWKKMGKAKQPYLIRRRDRQPFAFAGLWSSWKNPADPARPLETYTILTTDANGTIR
                     ELHDRMPVILDRADFGLWLDPGVGETDRLQSLLVPAADDLLETFPVSRSVNSPAHDAA
                     DCIEPLVDHAPEEPRQASLL"
     gene            complement(5057..6367)
                     /locus_tag="VEL74_03700"
     CDS             complement(5057..6367)
                     /locus_tag="VEL74_03700"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HYG61661.1"
                     /translation="MAGVLEADWTWTGERFESGVRVRVGDDGRITTVGPLAEAPDRRL
                     EGQALLPGMVSAHSHAFQRGLRGRGETFPGESGSFWTWRRAMYGLVEDLDPDAFEGLC
                     LRTFREMRRAGITCVGEFHYFHHRRIGDGSGDHAMDERVLRAAAAAGIRIALLQVYYR
                     TGGIGQPLEGGQLHFASPSPAAYWEQMARLAEAMGRIDPLRQSLGASVHSLRAAGPDE
                     LAAIYDEARRRDLPFHIHVEEQRREIEDSLAFYNRHPMAVLLDALGTATDVTAVHCTH
                     TDGEDMERFLAGGGTVCICPLTEGNLGDGIAALPRIHTLAGQAGAVCLGSDSNARISM
                     LEEMRWLEYVQRLATESRGVLRDGDGSVGRVLFRAATETGARALRVDAGRIAPGCWAD
                     FVTLDLEAPTLAGWTPDTLLDALIFGAGEEAIAGTCVAGVWEER"
     gene            complement(6367..>6939)
                     /locus_tag="VEL74_03705"
     CDS             complement(6367..>6939)
                     /locus_tag="VEL74_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYG61662.1"
                     /translation="LGVNAIEPAGRAIAALARLRGELEGEEVPHAELFPEVPFVPLNV
                     GTVHGGSAVNVVPDRCVVEVGVRPLPGVSSEELLARIARVVREDAAVAGFTDLDIEML
                     SDSPPLVLDRDAPIHRHLCSLVGQAGDASVSFATDAGWLQRLGLDCAIFGPGSIEVAH
                     KPNEWIPKDQLQAARGLLEHTVHHFCREAP"
BASE COUNT          929 a         2430 c         2517 g         1063 t
ORIGIN      
        1 cgttggcgcg gcggctcagg ttgagcgcca gcacggtggg gcagaacggc gcgccgcggt
       61 cgcccggccg ctcggagccg aggtcgagga cctcgtccat cggcatgccg aggccggcgg
      121 cgaggggctc caggtggcgc gccgccaggt cgggacggaa gcggtcgtga cccgcctcga
      181 ccggcgtgtg ggtggtgaag gcgctggtcg ccgccacctc gcggatggcg tcggtggcgg
      241 agagcccctg gtgctggacc atgtgccgcg cccgctcgag cagcgcgaag gcggagtgcc
      301 cctcgttcag gtgcaggacc gagggctcga tgcccagggc gcccagggcg cggaagccgc
      361 cgacgccgag caggatctcc tgctggatcc gggtctcctg gtcgccgccg tagaggcgcg
      421 cggtcagctc ctggtcctcg ggggtgttgg cctcgtcgcg ggcatcgagg agcaggaggt
      481 cgttgcgccc gacgtgcacg cgccagatgc ggagccgcac gttgcggccc ggcagctcga
      541 cgtcgacgaa cgcctgccgg acgccgccct cgctatcccc gtcgctgtcg ccgccgccgt
      601 tctcaccgga caaggcgggc acgggcttga ccggcaactc ggcggcggcg atgggctcgt
      661 agaggtcctg ctgccagccg ttctcgtcga tgagctggtg gacgtagccc tggtggtaga
      721 ggaggccgac gcccaccacc cgcaccccga ggtcgctcat gctcttcagg tggtcgccgg
      781 cgagcacccc caggcccccc gagtagatgg gcagcgactg gtggagcccg aactcggcgg
      841 agaagtaggc caccggccgg gcctggagcg ggccggcgtg agtcagcccc cagccttcgt
      901 cggcgcgcag gtactcgacg agccgccggt gggcctggtc gatgcgggtc tggatctcga
      961 ggtcggccac ccggcgggcg atctcctcgt cgctcaggcg ctgcagcatg gcgaccgggt
     1021 tgtggtagac cagccgccag agtacggggt ccagggtctt gaacaaggtg cggatgtcag
     1081 gctgccagga ccaccagagg ttctgggcga gctcgcgcag cttgcggagt gtcgcgtcgc
     1141 aggaaggatc ggtcatcgat tagctccatt gcatacggcc gggcctgccc cttccacctc
     1201 tcccaccctg ggggcccgcg gacgtgccgt cgagcggcaa ctcggtcttg aaccgggggc
     1261 aatatagcgc gaaccctccg gcgggggaaa gcgcgccgga gggcgatagc gggaaacggc
     1321 cgcgaggacc ggctagctcg gggactccgg cgggtcctcg ccgtaggtgg actcgtactc
     1381 catgttgccg tccttcttgt ggaccttgat ctggccgagg ggccccttct gggccagctt
     1441 gcgggcctcc tggacggcct cttccttggt gtcgtgctcg gtccggtagt cgccgttctc
     1501 ctgcgtcacc agccacttct cggccgacga gttggggacg acgtgataga ccttcctctc
     1561 ggtcttctct gctgtggctt tggccatcgg ttcacctcct ctgcccggtt tgccaggagc
     1621 aatcttcgtg ccgggccacg aatgctagac tgcgcccggt catctctgga ggtcgaatga
     1681 aggctcacac gccgcgcctc acccggtcgt ctttccttgc cgtcctgctg ctttccctgt
     1741 ccctggtcta cccggcggca gcccccgcgc aagaggcaaa ggggggcttt accctggagc
     1801 aggtgctctc cgcgccgttc ccctcggacc tgacagccgc cggcaccggc ggccaggtcg
     1861 cctgggtgca gaacgcccgc ggggtgcgca acctctggat cgccgccccc ccggactacc
     1921 gcgggcggca agtcacctcc tatacccggg acgacggcca ggccctctcc ggcctcgcct
     1981 ggagcgccga cggcaagacc ctcttctacg tgcgcggcag cggtgccaac cgccagggcg
     2041 aggtgcccaa ccccgccggc gacccgggcg gcgccgagca ggccgtctgg cgcatctccg
     2101 ccgaaggggg tgagccggtg cgcatcggcg tggggagcca gccgaccccc tcgcccaagg
     2161 gggacggcgt cgccttcgtc cgccgcggcc agatcttctg ggcgcccctc gcctcggacg
     2221 gtgccgaggg cggcaagagc gccgagccgg tgcagtggct gcaggcgcgg ggcacggcct
     2281 cggcgttgcg cttttcccca gacggctcga agctcgcctt caccagcggc cgcggcgacc
     2341 acaacttcat cggcgtctac gacgtggcgg cgaaggccgt gcgctggatg gcccccggcg
     2401 tcgaccagga cggctccccc gcctggtcgc cggacggcac gcgcctcgcc ttcgtccgcg
     2461 tgcccgccca gcgccggacc cgccttttcc tctccaaccg tagcggtcat ccctggtcga
     2521 tcctggtcgc ggaccccgcc accggtcagg cgcggacggt ctggcgggcg cggcccgggg
     2581 acggcagcgt cttccgcgtc gtggtggcgg acgaccagct cctctggggc gccggcgacc
     2641 ggctggtctt cccctccgag gcggacggct ggctccacct ctactccgtg cccgtcgccg
     2701 gaggggaggc gaccctgctg accccggggg agttcgaggt cgagtacgtc acactgtcgc
     2761 cggaccgccg gaccgtcctc tacaactcca accaggggga catcgaccgc cgtcacctct
     2821 ggcgggtgga cgtggcgggc ggcacgccgg ccgccctcac ccggggggac ggcatcgagt
     2881 ggtcgcccac ggctgtcggt aactctctgg cctatctgcg ctccggtgcc cgccgccagg
     2941 cgcacgccgt catccaggtg gggaatggcg agccgcggga gctggcaccg ggtacggtcc
     3001 ccgcggagtt ccccgaggcg gcgctggtgg agcccgagcc ggtggtcttc acctccggcg
     3061 acggcatgcg gatccacgcc cagctcttca agccccgaga tatccggcag ggggagcggc
     3121 ggccggcgct cctcttcctc catggcggct cgcaccgcca gatgctgctc ggctggcact
     3181 acagcggcta ctacaacaat acatacgcca tgaaccagta cctggccggc cggggttacc
     3241 tggtgctggc ggtcaacttc cgggccggca ccggctacgg gatgcatttc cgtgaggccc
     3301 tgcgccaggg ggcgggcggc gccagcgagt tcgccgacgt gctgggggcg gcgctctacc
     3361 tgcgcggccg tcccgacgtc gatccgcggc gcatcggcct ctggggcggc tcctacggcg
     3421 ggtacctcac cgcgatgggc ctcgcccgcg cgccggagct cttcgccgcc ggggtcgaca
     3481 tccacggcgc ccacgactgg aacgacgaga tcctcaactt cgtcccgagc tacgagccga
     3541 cgcccgagga ggagcgcctc gccttccagt cctctcccat ggcctggctg gacctctggc
     3601 gcgcgcccgt cctcctgatc cacggcgacg acgaccgcaa cgtcccgttc agcgagtcgg
     3661 tgcggctcgc cgaggcgttg cgcgagagag gcgtcgaggt ggaagccgtg gccatacccg
     3721 acgagatcca tggtttcctg cgctaccaga gctggctcga cgtgttccgg gccgcggggg
     3781 acttcttcga ccgcaagctg aaggcgaaga cggactagac aaccgaagga ggggtagcga
     3841 tggaaggcat ggcgggtctc ctgtgggtcc tcgcggtcat cctggtggtc gtcggagtgg
     3901 cggggacggt gctgccggca ctgcctgggg cgccgctggt tttcgccggc ctggtggtgg
     3961 cggcctgggc cgagggcttc gagaaggtgg ggtggatcac cctgacgctc atcggcctgc
     4021 tgaccctgct caccttcgcc gtcgacttcc tggcgagcac gctgggggcg aagcgggtcg
     4081 gggcgagctg gatggccgtc gtcggtgcca cgctcggcat cgtggccggc ttctttctgg
     4141 ggctgcccgg cctgctgctc ggccccttcg tcggcgcttt cctgggcgag tacctggcgc
     4201 ggcgcgactg ggctcaggcg cggcgcgctg gcatcggcac ctggatcggc ttcctgctcg
     4261 gcaccgtgct caagctcacc ctgatcttcg ccatgatcgg gctcttcgcc gccgcctatt
     4321 tcttctaggg ctacagcagc gaggcctgcc gcggctcctc gggggcgtgg tcgaccagcg
     4381 gctcgatgca gtccgcggcg tcatgggccg ggctgttgac cgaccggctc accgggaagg
     4441 tctcgagcag gtcgtccgcc gctggcacca gcaaggactg gagccggtcg gtctcgccta
     4501 caccggggtc gagccagaga ccgaaatcgg cgcggtcgag gatcaccggc atgcggtcat
     4561 ggagctcgcg gatcgtgccg ttggcgtcgg tggtgaggat ggtgtaggtc tcaaggggcc
     4621 gggcggggtc agccgggttc ttccacgacg accagaggcc ggcgaaggcg aacggctggc
     4681 ggtcgcgccg gcggatgagg tagggctgct tggccttgcc catcttcttc cactcgtaga
     4741 agccgtcggt caccaccagg caacgcttct tcttgaagct cgtacggtag gcgggcttct
     4801 cggcgacact ctccgagcgg gcgttgatca tccggttgcc gatcgccgga tcgtcggccc
     4861 agtaggggac gagcccccag cgcagatagg agaggacgcg cggcccgccg ggctcgagca
     4921 tccgcaccac cggcgcgtcc tgggtcggcg ccatgttgta gcgagcctgg aggtccggca
     4981 tctccatgag atcgaagagg gtggcgacct gctcacccga agaggtcaag gtatagcgtc
     5041 cgcacatgga gtgattctat cgctcctccc acaccccggc tacgcaggtg ccggcgatgg
     5101 cctcctcgcc ggcgccgaag atcagggcgt cgaggagggt gtcgggggtc cagccggcga
     5161 gggtaggggc ttcgaggtcg agggtgacga agtcggccca gcagccgggg gcgatgcggc
     5221 cggcgtcgac cctcagcgcc cgcgcccccg tctcggtggc ggcccggaag agcacccggc
     5281 cgacgctgcc gtcgccgtcg cgcagcacgc cgcggctctc cgtcgccagc cgctgcacgt
     5341 actcgagcca gcgcatctcc tccagcatcg agatgcgggc gttggagtca gagcccaggc
     5401 agaccgcgcc cgcctggccc gccagggtgt ggatcctggg cagggcggcg atgccgtcgc
     5461 cgagattccc ctcggtgagc gggcagatgc agaccgtgcc gccgccggct aggaagcgtt
     5521 ccatgtcctc gccgtccgtg tgggtacagt ggacggcggt gacgtcggtc gccgtgccca
     5581 gggcgtcgag caggacggcc atcgggtgcc ggttatagaa ggccagggag tcctcgatct
     5641 ccctgcgctg ctcctcgacg tggatatgga aggggaggtc ccgccgccgc gcctcgtcgt
     5701 agatggcggc cagctcgtcg ggaccggcgg cacgcaggct gtgcaccgac gcccccaggc
     5761 tctgtcgcag gggatcgatc cgccccatcg cctccgccag ccgcgccatc tgctcccaat
     5821 aggccgctgg cgacggcgag gcgaagtgga gctgcccccc ctcgagcggc tgtccgatgc
     5881 cgccggtccg gtagtagacc tggaggaggg cgatgcggat gccggccgcc gcggcggcgc
     5941 ggagcacccg ctcgtccatt gcgtggtcgc cgctgccgtc gccgatccgc cggtgatgga
     6001 agtagtggaa ctcgccgacg caggtaatgc ccgcgcgccg catctcgcgg aaggtgcgca
     6061 ggcagagccc ctcgaacgcg tcggggtcga ggtcctcgac cagcccgtac atcgcccggc
     6121 gccaggtcca gaagctgccg ctctcgccgg ggaaggtctc gccccgcccg cgcagcccgc
     6181 gctggaaggc gtgggagtgg gcgctcacca tgccggggag gagggcctgt ccctccaggc
     6241 gccggtccgg agcctctgcc agcgggccca ccgtcgtgat acggccgtcg tcgccgacgc
     6301 gcacccgcac gccggactcg aagcgctcgc cggtccaggt ccagtccgct tcgagaactc
     6361 cggccatcac ggggcctccc tgcagaagtg gtggaccgtg tgctcgagga gaccgcgggc
     6421 cgcctggagc tggtccttgg ggatccactc gttgggcttg tgcgccacct caatcgagcc
     6481 agggccgaag atggcgcagt ccagccccag gcgctgcagc cagccggcgt cggtggcgaa
     6541 ggagacgctt gcgtcgccgg cttggccgac cagcgagcag agatggcggt ggatcggggc
     6601 gtcccggtcg agaaccaggg gcgggctgtc tgacagcatc tcgatgtcca ggtccgtgaa
     6661 gccggcgacc gcggcatcct cccgtaccac acgggcaatg cgggcgagca gctcctccga
     6721 gctcaccccg gggagggggc gcacgccgac ctcgacgacg caacggtcgg ggaccacgtt
     6781 aaccgccgag ccgccgtgca ccgtgcccac gttcagcggg acgaagggga cctcggggaa
     6841 gagctcggca tgcggcacct cctccccttc gagctcgccg cgcaggcggg cgagggcggc
     6901 gatggcccgc ccggccggct cgatggcgtt gacgcccag
//
LOCUS       DASPJI010000135         6797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_636452, whole genome shotgun sequence.
ACCESSION   DASPJI010000135 DASPJI010000000
VERSION     DASPJI010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636452"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..>6797
                     /locus_tag="VEL74_03710"
     CDS             32..>6797
                     /locus_tag="VEL74_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61663.1"
                     /translation="MAPRTPVEEVVAGLWAEFLGVERVGAADSFFDLGGHSLLATRVI
                     SHLQAVLGVEMSLRDLFEAPTLSNFAAHVDAARRAGAVQSAPPLVPVPREGPLPLSFA
                     QQRLWFIDQLKPGTSLYNVPVTLRVEGPLHLSVLSLCLSEIVRRHEALRTVFAAPAGS
                     PVQVIQPAAPFVLPVVDLSGLPESRREAPAILLARTEAGRPFDLRRGPLLRGLLVRLD
                     EQDHITVLTMHHIVSDGWSMGILVREVVALYGAFAEGRPSPLPEPPLQYADFAVWQNT
                     WLSGEVLEGEIDYWRRQLAGLPPLLEFPTDRPRPPVQSYRGAIRPVRLPSELTRQVQA
                     LGRREGATLFMVLLAAFQALLARYSGQQDLAVGSPIAGRNRAEIEGLIGFFVNTSVLR
                     GDLTGEPSFRELLGRARETALAAYLHQDMPFEKLVLELTPDRSLAHSPLFQVMLVLQN
                     APVESLEIKDLRLQPVNGAGTTAKFDFTLNLVEWEGELTGTLEHATDLFDGTTMDHLA
                     EHFERLLAEAVAAPERPVWELPLLGEAERQLTLVEWNDTAVEHGVLPLLPRLLEARVA
                     HGPDGIAVEQGDLSLTYRELEARANRLAHLLIRHGVTPEDRVAICCDRSPEMVVSQLA
                     TWKAGAAWVPLEPSFPSARRATVLDDARPAVLIAGPGAPADLSQKDGNPALRVLDLDA
                     VAAELAGAAGEDPGPPALPLTPDYLSYVIYASGSTGRPKGVLVAHGAIANRLLWMRRA
                     LSLSALGPVGEQDAVLQKTPFVFDAAIWEMALPLLTGARLVLAPPGAHREPAVMAREV
                     LEHGVSLLQLVPSALGPFLDEDLHGSPLRRLFCGGEALPAPLCQRAFERLPGIELRNL
                     YGPTECAIDVTSRPCRPGEEGTIVPLGRPVDNLRIRILDRRLQPVPAGQPGELCAGGA
                     GVARGYLGRSDLTAERFVPEPLGLVPGGRLYRTGDLVRTLPGGAIEFLGRLDDQVKIR
                     GLRIELGEIEAALVGLPEVSQAVATVREDVPGDRCLVAYVVRRPADGEALQASQPESA
                     ASEHVAEWQTLYEETYGRQIQDVDPTFNTEGWNSSYTGEPIPAEEMREWVDATVERIL
                     GLGRRRVLEVGCGTGLLLFRIAPHSELYLGTDFSRGALDFVQRHLEHAERAGRALPQV
                     SLAQRTADDWTAVPQGDIDLVVINSVAQYFPDIDYFVRVLERAVRTLAARPEGGSVFL
                     GDMRSLPLLEALHTSIELFQAPPSMATEELRRRVRRRVANEEELVVDPELFFALARWM
                     PEIRRVEILVKRGRYHNELTRFRYDAVLHVGGAEERRETPAFPPFDWREQDLSLAACE
                     RLLATDGPAALVLTGIPNGRLATESAALDLLAAPGQAGEMVEDLRQAIARRLPLAPQP
                     GAAVDPEEIGAMGERLGYVVELVWAVGDGAREGWFAAVLRRPGVTVSSPPDFLPAHDA
                     ALRPWSAYANDPLRGRVARRLVPELRRALQAELPEYMIPSSILLLDSLPLTSNGKVDR
                     NALPAPEILRSVPGGYVPPRTPTEEVISQIWAELLGIERVGATDHFFELGGHSLLATQ
                     VVSRLRSVYSIEMPVRDLFAAPVLADLAARIEEARRAGAAPSAPPLVPAPRQGPIPLS
                     FAQQRLWFIDQLEPGTSLYNIPVALRAEGPLHLGVLMLCLSEIVRRHEALRTVFAAPE
                     GTPTQVILPAAPFVLSVVDLSGLPESRREALALGLVGEEAGRPFDLTRGPLLRGLLLR
                     LAEDDHITVLTMHHIVSDGWSMRILVREVTALYAAFTEGRPSPLPELPLQYADFAVWQ
                     RSWLHGEALERESSFWRRQLAGLPPLLELPTDRPRPLVQSFRGASRPMRLPAELTRQM
                     DAVGRREGATIFMVLLAGFQLLLSRYSGQQDLAVGTPAAGRNRTEIEGLIGFFVNTLV
                     LRGDLSSGRSGEISFRELLGRVRESALAAHAHQDVPFEKLVQELSPERTLAYTPLFQV
                     MLVLQNVPAEALEIQSLRLRPTSVAGAVARFDLTLALGEHNGELMGGLSYATDLFDAA
                     TIDRLSGHLERLLAAAAATPDLPVSALPFLSPAELHELAQEAGRLGMAPDTDGALGPE
                     RCVHELFAERASRTPEALAVVDEERAWTYAELLRRVRRLALRLRAAGIGPDQPVILCA
                     ERGAGLVTGLLGILEAGGAYLAVEPDLPHARQELLAWDARVAVAVTESRLTGTLPTGL
                     RQVFLDADDLEGAEDSELPVGGVPRAVVTPGNLAYVLYTSGSTGAPKGVMVEHRQLMA
                     YVRGALE"
BASE COUNT          902 a         2438 c         2388 g         1069 t
ORIGIN      
        1 gcccgagaag cagggctccg aggagggttg ggtggcgccc cgcacgcctg tcgaggaggt
       61 cgtcgccggc ctctgggccg agttcctcgg cgtcgagcgg gtgggggccg ccgattcctt
      121 cttcgatcta ggcggacact cgctgctggc gacgcgggtg atctcgcacc tgcaggcggt
      181 cctcggggtc gagatgtcgc tgcgcgacct cttcgaggcc ccgacgctgt cgaacttcgc
      241 ggcccacgtc gacgcggcgc ggcgggcggg agcggtgcag tcggcgccgc ctctggtccc
      301 ggtcccccgg gaagggccgc tccccctctc gttcgcccag cagcgcctct ggttcatcga
      361 ccagctcaag cccggcactt cgctctacaa cgtgccggtg acgctacggg tcgaggggcc
      421 tctgcacctc agcgtgctgt cgctctgctt gagcgagatc gtgcgccgcc acgaggcgtt
      481 gcgcacggtc ttcgccgcgc cggcgggctc gccggtgcag gtgatccagc ccgcggcccc
      541 cttcgtcctg ccggtggtgg atctgtcggg gctgccggag agccggcgcg aggccccggc
      601 catcctcctc gcccgcaccg aggccggccg cccgttcgac ctgcgccgcg ggccgctgct
      661 gcgcggcctg ctagtgcgcc tggacgagca ggaccacatc accgtgctga ccatgcatca
      721 catcgtcagc gacggctggt ccatgggtat cctggtgcgc gaggtcgtgg ccctgtacgg
      781 cgccttcgcc gagggccgac cgtcccccct gccggagccg ccgctgcagt acgcggattt
      841 cgcggtctgg cagaacacct ggctgagcgg cgaggtcctg gaaggcgaga tcgactactg
      901 gcgccgccag ctcgccggcc tgccgccgct cctcgagttc cccaccgacc ggccgcggcc
      961 accggtgcag agctaccgcg gcgccatccg gccggtgcgg ctgcctagcg agctcacccg
     1021 gcaggtgcag gcactgggcc gccgcgaagg cgcgacgctc tttatggtgc tgctcgcggc
     1081 cttccaggcc ctcctcgccc gctacagcgg ccagcaggac ctggccgtgg gctcccccat
     1141 cgccggccgc aaccgggccg agatcgaggg gttgatcggc ttcttcgtca acacctcggt
     1201 gctgcgcggc gacctgaccg gcgagccctc gttccgtgag ctgctcggcc gggcgcgcga
     1261 gacggcgctc gccgcctacc tgcaccagga catgccgttc gagaagctgg tcctggagct
     1321 cacccccgac cggagcctcg cccattcccc cctgttccag gtgatgctcg tgctgcagaa
     1381 cgcccccgtc gagagcttgg agatcaagga tctgcgcctg cagccggtaa acggcgccgg
     1441 gaccacggcg aagttcgact tcacgctcaa tctcgtggag tgggaggggg agctgactgg
     1501 cacgctcgag cacgccaccg atctgttcga cggcacgact atggatcact tggccgagca
     1561 cttcgagcgg ctgctggccg aggccgtggc ggcgccggag cggccggtct gggagctgcc
     1621 gctgctcggc gaggcggagc ggcaactgac gctggtggag tggaacgaca ccgcggtgga
     1681 gcatggcgtg ctgccgctcc tccctcgcct gctggaggcc cgggtcgccc acggcccgga
     1741 tggcattgcg gtagaacagg gggacctctc cctcacctac cgcgagctgg aggcgcgggc
     1801 gaaccggctg gcccaccttc tgatacgcca cggagtcacc cccgaagacc gggtggcgat
     1861 ctgctgcgac cgctcacctg agatggtggt gagccagctc gcgacgtgga aggccggcgc
     1921 ggcgtgggtc cccctggagc ccagcttccc ctcggcgcgg cgggccaccg tgctggacga
     1981 cgcccgcccg gccgtgctga tcgccgggcc gggcgccccc gcggatctgt cccagaagga
     2041 cggcaacccc gcgctccgcg tcctcgatct cgacgcggtg gcagcggagc tggcgggcgc
     2101 cgccggggaa gaccccggac cgccagcgct gcccctcacg ccggactatc tctcctacgt
     2161 catctacgcc tcgggctcga ccggccggcc caagggggtc ctggtggcgc acggcgccat
     2221 cgccaaccgc ctcctctgga tgcggcgcgc actctctctc agcgctctgg gccccgtcgg
     2281 cgagcaggac gcggtgctcc agaagacgcc gttcgttttc gacgcggcga tctgggagat
     2341 ggccctcccc ctgctcaccg gcgcgcggtt ggtgctggct ccgcccggcg cccatcggga
     2401 gcccgccgtc atggcgcggg aggtgctgga gcacggcgtc tccctcctcc aactcgtccc
     2461 gtccgccctc ggcccgttcc tcgacgagga cctgcacggc tcccccctgc gccgcctgtt
     2521 ctgcggcggc gaggccctgc cggcgccgct ctgccagcgg gcgttcgagc ggcttcccgg
     2581 gatcgagctg cgcaacctct acggccccac ggagtgcgcc atcgacgtaa cctcccgccc
     2641 ctgccggccg ggcgaggagg ggaccatcgt gccgctcggc cggccggtgg acaacctgcg
     2701 gatccggatc ctcgaccgca ggctgcagcc cgtgcccgcc ggtcagcccg gcgagctgtg
     2761 cgccggcggc gccggagtgg cgcggggcta cctcggccgg tcggacctca ccgccgaacg
     2821 cttcgtgccc gagccgctcg ggctcgttcc cggcgggagg ctctatcgca ccggcgacct
     2881 ggtccgcacc ctgcccggcg gcgccatcga gttcctcggc cgcctggacg atcaggtcaa
     2941 gatccggggc ctgcgcatcg agctggggga gatcgaagcg gcgctcgtcg gactgcctga
     3001 ggtgagccag gccgtggcca ccgttcggga ggacgtgccc ggcgaccggt gcctggtcgc
     3061 ctacgtggtg cgccgacccg cggacggcga agctctccag gccagccagc cagagtccgc
     3121 cgccagcgag cacgtggcgg agtggcagac gctctacgag gagacctacg gccggcagat
     3181 ccaggacgtc gatcccacct tcaataccga ggggtggaac agcagctaca cgggcgagcc
     3241 gatcccggcc gaggaaatgc gggagtgggt cgacgccacg gtggaacgga tcctcggcct
     3301 tggccgccgg cgcgtcctcg aagtggggtg tggcaccggt ctgctgctct tcaggatcgc
     3361 gccccacagc gagctctacc tgggcacgga cttctcccgc ggcgccctgg acttcgtgca
     3421 gcggcacctg gagcacgccg aacgggcggg cagggcgctg ccgcaggtgt cgctggcgca
     3481 gcgcaccgcc gacgactgga ccgcggtgcc gcagggggac atcgacctcg tcgtcatcaa
     3541 ttcggtcgcc cagtacttcc ccgatatcga ctacttcgtt cgggtcctcg agcgggcggt
     3601 gcggaccctg gcggcccgcc cggaaggggg ctctgtcttc ctcggcgaca tgcgcagcct
     3661 gcccctgctg gaggccctgc acacctccat cgagctcttc caggcgccgc cgtcgatggc
     3721 gacggaggag ctgcggcgcc gggtgcggcg ccgggtcgcc aacgaagagg agctggtggt
     3781 ggatcccgag ctgttcttcg ccctggcccg gtggatgccc gagatccggc gcgtcgagat
     3841 cctggtcaag cggggccggt accacaacga gctcactcgc ttccgctacg acgccgtcct
     3901 gcatgtcggc ggcgcggagg agcggcggga gaccccggcc ttccccccgt tcgactggcg
     3961 cgagcaggac ctctcgctcg ccgcctgcga gcggctgctg gccacggacg gaccggccgc
     4021 cctggtcctg accggcatcc ccaacggccg tctggcgacg gagagcgccg ccctcgacct
     4081 actcgccgcc cccggccaag cgggcgagat ggtggaggac ctccggcagg ccatcgcacg
     4141 gcgtctcccc ctggcgcctc aaccgggggc ggccgtggac ccggaggaga tcggggcaat
     4201 gggcgagcgg ctcggctatg tcgtggagct ggtctgggcc gtgggggacg gtgcccggga
     4261 gggatggttc gccgcggtgc tgcggcggcc cggcgtgacg gtctccagcc cgccggattt
     4321 cctgccggcc cacgacgctg cgctccgtcc gtggagcgcc tatgccaacg acccgctgcg
     4381 cggacgggta gcccgccgcc tggtgccgga gctgcggcgc gccctgcagg cggagctgcc
     4441 ggagtacatg atcccctcct cgatcctgct gctggacagc ctgcccctca cctcgaacgg
     4501 caaggtggac cgcaacgcgc tgcccgcgcc ggagatcctg cgctccgtgc ccggcggcta
     4561 cgtgccgccg cgcacgccga ccgaagaggt gatcagccag atctgggccg agctcctcgg
     4621 tatcgagcgg gtcggggcga ccgatcactt cttcgagctg ggcgggcatt cgctgctcgc
     4681 tacgcaggtg gtctcccgcc tgcgtagcgt ctacagcatc gagatgccgg tacgcgatct
     4741 attcgccgcg ccggtgctgg cggacctcgc ggcccggatc gaagaggcgc ggcgggccgg
     4801 cgcggcgcct tcggctccgc ctctggtccc ggcgcctcgc cagggaccga ttcccctctc
     4861 gttcgcccag cagcgcctct ggtttatcga ccaactcgaa ccgggaacct cgctctacaa
     4921 catcccggtg gcgctgcgtg ccgaagggcc gctgcacctc ggagtgctga tgctctgcct
     4981 gagcgagatc gtacgccgcc acgaggcgtt gcgcacggtc ttcgccgcgc cagaaggtac
     5041 tccgacgcag gtgatcctac cggcggcccc tttcgtcctg tccgtagtgg atctgtcagg
     5101 ccttccggag agccggcgcg aggctctggc cctcggcttg gtcggcgaag aggcaggccg
     5161 cccgttcgac ctgacgcgtg ggccgctgct gcgtggcctg ctgctgcggc tggcggagga
     5221 cgaccatatc accgtgctga ccatgcatca catcgtcagt gacggctggt ccatgaggat
     5281 cctggtccgc gaggtcacgg ctctctatgc ggcgtttacc gagggcaggc cctcgccttt
     5341 gccggagctg ccattgcagt atgccgattt tgcggtctgg caacgctcct ggctgcacgg
     5401 agaagcccta gagagagaga gctccttctg gcgccgccaa ctcgcgggac tgccgccgct
     5461 actggagctc cccaccgacc ggccacggcc cctagtgcag agcttccgcg gcgcttcacg
     5521 gccaatgagg ctgccggccg agctgacccg gcagatggat gccgtgggcc gtcgcgaggg
     5581 cgctacgatc ttcatggtgc tgctagccgg cttccagctc ctcctgtccc gctacagcgg
     5641 gcaacaggat ctcgcggtag gcacaccagc cgccggacgc aaccggacgg aaatcgaagg
     5701 cttgatcggg tttttcgtca atacactggt gctgcgtggc gacctgagca gcggcagatc
     5761 cggagaaatc tcgttccgcg agttgctcgg ccgggttcgc gaatccgctc tggccgccca
     5821 tgcgcaccag gacgtgccgt ttgaaaagtt ggtgcaggag ctctcgccgg agagaaccct
     5881 ggcctacacc cccttgttcc aggtgatgct cgtgttgcag aacgttcccg ctgaagcgct
     5941 agagatccag agcttgcggc tgcggccgac gagcgttgcc ggggcggtag caaggttcga
     6001 tctcactctt gcgcttgggg agcataacgg cgagttgatg ggtgggctct cctacgccac
     6061 cgatctattc gacgctgcga ccatcgaccg cctgagcggt catctcgagc ggctgctcgc
     6121 cgccgcggcg gccacgccgg acctgcccgt ttctgcgctg cctttcttga gcccggccga
     6181 actgcacgag ctcgctcagg aggccggaag gctcgggatg gcgcctgata cggacggcgc
     6241 tctcggaccg gagcgctgtg tccacgagct cttcgctgag cgggcgtcgc gcactcccga
     6301 ggcgctcgcc gtggtcgacg aggagcgcgc gtggacttac gcggagctgc tccgccgggt
     6361 gcggcggttg gctttgcggc tacgcgccgc ggggattggc cccgaccagc cggtaattct
     6421 ctgcgccgag cgcggcgcgg gtctggtaac cggtctcctc ggcatcctgg aggcgggagg
     6481 ggcctatctg gcagtggagc cggacctgcc ccacgcccgg caggaactcc tcgcctggga
     6541 tgctcgtgtg gccgtggccg tgaccgagag ccgactgacc ggcacgttgc ctaccggctt
     6601 gcgacaggtc ttcctggacg cagacgacct ggagggagcg gaggactcgg agctgcccgt
     6661 cggaggagtg ccgcgcgccg tggtcacgcc aggaaacctg gcctacgtgc tctacacctc
     6721 gggctcgacg ggcgcgccga aaggagtcat ggtcgagcac cggcagctca tggcgtatgt
     6781 tcgcggtgcc ctggaac
//
LOCUS       DASPJI010000136         5403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_693933, whole genome shotgun sequence.
ACCESSION   DASPJI010000136 DASPJI010000000
VERSION     DASPJI010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693933"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="VEL74_03715"
     CDS             complement(<1..1569)
                     /locus_tag="VEL74_03715"
                     /inference="COORDINATES: protein motif:HMM:NF027900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HYG61664.1"
                     /translation="MRTEATVSAALAVALLLAGAVSLPAAVSTPAVPRFGTAEVVLRA
                     SQTFDSRQGSPNPFDVTLTATVTAPSGKIYQADGFFDGDGAGGPVGNVFKVRIYAAET
                     GTWSWSTASSRAELAGRTGTFTCAGTLPGPFASGPLAVNPRRPHTFRYQEGRPVYLVG
                     KFLDLAAPPVLQYSHTFFSEQLTEADRQAFLDRHLGMGLNKINLYLANHGDYDGVATT
                     PWVGVKGANDKRRFDLARWHMYERWLRRLRDAGLLAHLWFFADDSGFGDLPDADRQRL
                     VRYGMARLSGEVHTLFTLALEWQEGWTPAEVQSHSNFLQQHNPWDRLVSVHGVPGPFD
                     FPAAPWADYMDVQAGNEVGPSAVYAAGVLNRALAAKPLIQEEFGLGREVAEERRKAWA
                     AFLSGAAGSGTGAFLRPLAQFTNSIQFQRLAPAPTVVLAGGAWALAAPGETYVLYLHD
                     GGTVQVSLLGVSGTFEAEWFDPRRGTFTRGADVQGGGIRSFTAPGSGDWTLLLHRPAP
                     PPAPVPAHFHTLRPC"
     gene            1707..4937
                     /gene="carB"
                     /locus_tag="VEL74_03720"
     CDS             1707..4937
                     /gene="carB"
                     /locus_tag="VEL74_03720"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HYG61665.1"
                     /translation="MPRRNDIRSILVLGSGPIVIGQACEFDYSGTQACRVLRKEGYRV
                     VLVNSNPATIMTDPEFADATYVEPLVPEIVEKIIERERPDALLPTVGGQTGINLAIAL
                     EERGALRRYGVEVLGADIAALRLGEDRLLFKQAMEEVGLKVPRSGYAASLEEAREIAA
                     EIGYPLILRPSFTLGGEGGGTAYNRDELEEVVASALHASPSGQVLIEQSVLGWKEFEL
                     EVMRDLADNAIVVCSVENIDAMGVHTGDSITVAPQQTLSDREYQAMRDDAFKVIRRVG
                     VATGGSNIQFAVDPQTGARVVIEMNPRVSRSSALASKATGFPIAKIAALLAVGYTLDE
                     IANDITGKTFAAFEPTLDYTVVKIPRWAFEKFPSAATTLGTSMKSVGEAMAIGATFRE
                     ALMKGLASLELDGSLASREQKMSDPVLLRRRLAEPNWQRLFAVFAALRQGWTPEEVSE
                     VTHIDPWFLREMAVLVELEAELACWDLRSVPPALLRRAKQEGLTDSRIAALLHGQEAE
                     VRSLLAERGLRRVYKRVDTCAAEFPATTPYLYSTFGQEDESEPTENKKVVILGSGPNR
                     IGQGIEFDYCCVHAAFALSEAGFETVMVNCNPETVSTDYDTSDRLYFEPLTLEHVLAV
                     IERERPAGVIVQLGGQTPLKLARGLAAAGVPIWGTTPDAIDLAEDRKRFGALLAEEGI
                     LQPENGSATSLDEALEVARRIGFPVVVRPSYVLGGRAMAIVYDETTLERYMHEAADVS
                     PGHPVLIDRFLEDAFEVDLDLLADGERAVVAGVLQHIEEAGIHSGDSVAVLPPYKVSR
                     EDQDAMRDIARRLALRLGVVGLMNVQFALKDGRIYVLEVNPRASRTVPFIAKAVGVPL
                     VKIAVRAMAGESLASQGFTEEPAVPGVFVKAPVFPFRRFPGVDPVLGPEMKSTGEVMG
                     ASTAFGNAFAKAWLGAGHKLPLKGTAFLSVHDRDKPGVLPVARRLAELGFELRATSGT
                     AAYLAAHGLEVTPVLKVHEARPHIVDQMINGEIDLVVNTPLGRESFEDDGAIRRAALK
                     YDIPCITTLSGAAAAAEGIAAIADVERDGLAVRPLQERHAEVGVAG"
     gene            4954..>5403
                     /locus_tag="VEL74_03725"
     CDS             4954..>5403
                     /locus_tag="VEL74_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HYG61666.1"
                     /translation="MADTRIEKDSLGEVQVPAGAYYGAQTERARQNFPVSGIRLPRRF
                     VAAVALIKGEAAVVNQELGVIPGEIARAIAEAAVEVADGKLDDQFPLDIFQTGSATST
                     NMNVNEVLSNRAIEILGGVIGSKTPVHPNDHVNNGQSSNDVIPTAIHV"
BASE COUNT          803 a         1798 c         2066 g          736 t
ORIGIN      
        1 gcaggggcgc agcgtgtgga agtgggcggg gaccggggcc ggcggcggag cggggcggtg
       61 caggaggagc gtccagtccc ccgagcccgg cgcggtgaac gaccggatgc cgcccccctg
      121 gacgtccgcg ccgcgggtga aggtgccgcg ccgcgggtcg aaccattcgg cctcgaaggt
      181 gccggagacc ccgaggaggc tcacctgcac cgtgccgccg tcatggagat agaggacgta
      241 ggtctcgccg ggggccgcga gcgcccaggc gccgcccgcg agcacgaccg tgggcgccgg
      301 ggccaggcgc tggaactgga tggagttggt gaactgcgcc agggggcgga ggaaggcgcc
      361 ggtgcccgac ccggcggcgc cggagaggaa ggcggcccag gccttgcgcc gctcctcggc
      421 gacctcgcgg ccgaggccga actcctcctg gatcagcggc ttggcggcga gcgcccggtt
      481 cagcacgccg gcggcgtaga ccgcggaggg cccgacctcg ttgccggcct ggacgtccat
      541 gtagtcggcc cacggggcgg cggggaagtc gaacggcccg ggcacgccgt ggacgctgac
      601 caggcggtcc caggggttgt gctgctgcag gaagttggag tggctctgca cctcggcggg
      661 ggtccacccc tcctgccact cgagcgccag ggtgaacagg gtgtggacct cgcccgacag
      721 gcgcgccatg ccgtagcgga cgagccgctg gcggtcggcg tcgggcaggt cgccgaagcc
      781 ggagtcgtcg gcgaagaacc agaggtgggc gagcaggccg gcgtcgcgca gccgccgcag
      841 ccagcgctcg tacatgtgcc agcgcgcgag gtcgaagcgg cgcttgtcgt tggccccctt
      901 gacccccacc cacggggtgg tggcgacccc gtcgtagtcc ccgtggttgg cgaggtagag
      961 gttgatcttg ttgagcccca tgccgaggtg gcggtcgagg aaggcctggc ggtcggcctc
     1021 ggtgagctgc tccgagaaga aggtgtggga gtactgcagg acgggcggag cggcgaggtc
     1081 gaggaacttc ccgaccaggt agacggggcg cccctcctgg tatctgaagg tgtgggggcg
     1141 gcgcgggttg accgccagcg ggccggaggc gaaggggccg gggagggtgc cggcgcaggt
     1201 gaacgtgccg gtccgaccgg cgagctcggc gcggctgctc gccgtgctcc agctccaggt
     1261 gccggtctcg gcggcataga tgcggacctt gaagacattg cccacgggac cgccggcgcc
     1321 gtcgccgtcg aagaagccgt cggcctggta aatcttgccc gagggggcgg tgaccgtcgc
     1381 ggtcaaggtt acatcgaacg ggttggggga gccctgccgg gagtcgaacg tctggctggc
     1441 gcgcagcacg acctcggcgg tgccgaagcg cggcaccgcc ggtgtggaga cagcggccgg
     1501 gagcgagacg gcgccggcga ggagcagggc gacggccagg gcagcggaca cggttgcttc
     1561 cgttcgcatg ggcacactcc tgagggagag atcggcaggt ccggccgggc gcagacctcc
     1621 ggccgggtgg gcaatggctg tcgcggcatt cctgcaggaa gcaggccatg gcgggccgcc
     1681 cgccgatggg ctacacttcc cggccgatgc ccaggcgtaa cgatatccgc tccattctgg
     1741 tcctcgggtc cgggccgatc gtcatcggcc aggcctgcga gttcgactac tccggcaccc
     1801 aggcgtgccg cgtcctccgc aaggagggct accgggtggt gttggtcaac tccaacccgg
     1861 cgacgatcat gaccgacccg gagttcgccg acgccacgta cgtcgagccg ctggtgccgg
     1921 agatcgtcga gaagatcatc gagcgggagc gtcccgacgc cctgctgccc accgtcggcg
     1981 gccagaccgg catcaacctc gccatcgcgc tcgaggagcg cggggcgctc cggcgctacg
     2041 gcgtggaggt cctgggggcc gacatcgccg ccctgcgcct gggcgaggac cggctgctgt
     2101 tcaagcaggc gatggaggag gtggggctca aggtgccgcg cagcggctac gccgccagcc
     2161 tcgaggaggc gcgcgagatc gccgccgaga tcggctaccc gctgatcctc cggcccagct
     2221 tcaccctcgg cggcgagggg ggcggcaccg cctacaaccg cgacgagctg gaggaggtgg
     2281 tggcgtccgc gctccacgcc agcccgtcgg gccaggtgct gatcgagcag tcggtgctcg
     2341 gctggaagga gttcgagctg gaggtcatgc gcgacctcgc cgacaacgcc atcgtcgtct
     2401 gctcggtgga gaacatcgac gccatgggcg tccacaccgg cgactcgatc accgtggcgc
     2461 cgcagcagac gctgtcggac cgcgagtacc aggcgatgcg cgacgacgcc ttcaaggtga
     2521 tccggcgcgt cggcgtggcg accggcggct ccaacatcca gttcgcggtc gatccgcaga
     2581 ccggcgcgcg ggtggtcatc gagatgaacc cgcgggtgtc gcgcagctcg gccctggcgt
     2641 cgaaggcgac cggcttcccg atcgccaaga tcgccgcgct cctcgccgtc ggctacaccc
     2701 tggacgagat cgccaacgac atcacgggca agaccttcgc cgcgttcgag ccgaccctgg
     2761 actacacggt ggtcaagatc ccgcgctggg cgttcgagaa gttccccagc gccgccacca
     2821 ccctgggcac gtcgatgaag tcggtggggg aggcgatggc gatcggcgcc accttccgcg
     2881 aggcgctgat gaaggggctc gcctccctgg agctggacgg cagcctcgcc agccgcgagc
     2941 agaagatgtc ggacccggtg ctgctgcggc ggcgcctggc ggagcccaac tggcagcggc
     3001 tgttcgcggt cttcgccgcg ctgcgccagg ggtggacccc cgaggaggtg tcggaggtca
     3061 cgcacatcga cccctggttc ctgcgcgaga tggcggtcct cgtcgagctg gaggcggagc
     3121 tcgcctgctg ggacctgcgc tcggtgccgc cggcgctgct gcgccgcgcc aagcaggagg
     3181 ggctgaccga ctcgcggatc gccgcgctcc tccatggcca ggaggcggag gtccggtcgc
     3241 tgctggcgga gcggggcctg cggcgggtct acaagcgggt cgacacctgc gccgccgagt
     3301 tcccggcgac cacgccctac ctctactcga ccttcggcca ggaggacgag agcgagccga
     3361 cggagaacaa gaaggtggtg atcctgggct ccggtccgaa ccggatcggg caggggatcg
     3421 agttcgacta ctgctgcgtg cacgccgcct tcgccctgtc cgaggccggt ttcgagaccg
     3481 tgatggtcaa ctgcaacccg gagacggtgt cgaccgacta cgacacctcg gaccgcctct
     3541 acttcgagcc gctgacgctg gagcacgtgc tggcggtcat cgagcgcgag cgcccggccg
     3601 gggtcatcgt gcagctcggc ggccagacgc cgctcaagct ggcgcggggc ctggcggcgg
     3661 ccggcgtgcc gatctggggg acgacgccgg acgccatcga cctggcggag gaccgcaagc
     3721 gcttcggcgc gctgctcgcc gaggagggga tcctgcagcc ggagaacggc tcggccacgt
     3781 cgctggacga ggcgctggag gtggcgcgcc gcatcggctt cccggtggtg gtgcggccga
     3841 gctacgtcct cggcggccgg gcgatggcca tcgtctacga cgagacgacg ctcgagcgct
     3901 acatgcacga ggcggcggac gtctccccgg gccatccggt gctgatcgac cgcttcctcg
     3961 aggacgcctt cgaggtcgac ctcgacctgc tggccgacgg cgagcgcgcg gtggtggccg
     4021 gcgtcctcca gcacatcgag gaggcgggca tccactccgg cgactcggtg gcggtgctgc
     4081 cgccctacaa ggtgtcgcgg gaggaccagg acgcgatgcg cgacatcgcc cgccggctgg
     4141 cgttgcggct gggggtggtg gggctgatga acgtccagtt cgccctgaag gacggccgca
     4201 tctacgtgct cgaggtcaac ccgcgggcgt cgcggacggt gccgttcatc gccaaggcgg
     4261 tgggggtgcc gctggtcaag atcgcggtgc gggcgatggc gggggagagc ctggcgtcgc
     4321 agggcttcac cgaggagccg gcggtgcccg gggtgttcgt caaggcgccg gtcttcccgt
     4381 tccgccgctt cccgggtgtc gacccggtgc tggggccgga gatgaaatcg acgggcgagg
     4441 tcatgggggc gagcacggcg ttcggcaacg ccttcgccaa ggcctggctc ggggctgggc
     4501 acaagctgcc gctcaagggc acggccttcc tctcggtgca cgaccgggac aagccggggg
     4561 tgctgccggt ggcacgccgc ctggccgagc tgggcttcga gctgcgggcg acctcgggca
     4621 ccgcggcgta cctcgccgcc cacggcctgg aggtgacgcc ggtgctcaag gtgcacgagg
     4681 cgcgccccca catcgtggac cagatgatca acggtgagat cgaccttgtc gtcaacacgc
     4741 cgctgggccg cgagtccttc gaggacgacg gcgccatccg ccgcgccgcc ttgaagtacg
     4801 acatcccctg catcaccacc ctgtcgggag ccgccgccgc cgccgaaggc atcgccgcta
     4861 tcgccgacgt ggaacgcgac ggcctcgccg tgcgccctct tcaggagagg cacgccgagg
     4921 tgggggtggc ggggtaggat tccaggagag agcatggccg atacacggat cgagaaagac
     4981 agcctcggcg aggtgcaggt gccggccggg gcttattacg gcgcccagac ggaacgggcg
     5041 cggcagaact tccccgtctc ggggatccgg ctcccccggc gcttcgtcgc cgcggtggcg
     5101 ctgatcaagg gcgaggccgc ggtggtcaac caggagctgg gggtgatccc cggcgagatc
     5161 gcgcgcgcca tcgccgaggc ggccgtcgag gtggccgacg ggaagctgga tgatcaattc
     5221 ccgctggaca tcttccagac cggctcggcg acgtcgacca acatgaacgt caacgaggtg
     5281 ctgtcgaacc gggcgatcga gatcctcggc ggcgtcatcg gctcgaagac gccggtccac
     5341 ccgaacgatc acgtcaacaa cgggcagtcg agcaacgacg tcatccccac cgccattcac
     5401 gtg
//
LOCUS       DASPJI010000137         6986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_810190, whole genome shotgun sequence.
ACCESSION   DASPJI010000137 DASPJI010000000
VERSION     DASPJI010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810190"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /locus_tag="VEL74_03730"
     CDS             <1..1438
                     /locus_tag="VEL74_03730"
                     /inference="COORDINATES: protein motif:HMM:NF025499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HYG61667.1"
                     /translation="NPPPYTRVLRPCRPGDGIRQISTAEEHWLLARFEGAARDGRIGK
                     MVPASGAASRMFKSLLAYVNGSGGEPEGEVRTLFDNLERFPFYDALAAVMARGGHNLR
                     QAVDGGDYKTVLEYLLTPRGLGYEELPKGLLLFHHYPEGPRTPFEEHLVEAVEQTRSA
                     DGRCRLHFTVSPQHEEEFRRLLAEVGPRYEERYGVRFDVTFSYQKRSTDTLAVDPENR
                     PFRQDDGTLLFRPGGHGALLDNLDALGRNGWDIVLIKNIDNVVPEERRPLISQWKRLL
                     TGQLLAVQDRVWRLLERLEEGSTNALDEAAAFLEKELSRSLPAELAGVSEEVRRRRLI
                     DLLDRPLRVCGMVRNLGEPGGGPFWVESKTGEVSIQIVETSQLDPKSEEQQAQLAAST
                     HFSPTDLVCALRDRHGRPYDLHAYVDPSTVFMSTKSHEGRPLKALERPGLWNGSMAGW
                     SSVFVEVPDATFAPVKTVTDLLRPEHQG"
     gene            1561..2316
                     /locus_tag="VEL74_03735"
     CDS             1561..2316
                     /locus_tag="VEL74_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HYG61668.1"
                     /translation="MSADAKAAAPGGSGSRWKRVAYAGIAVAAVAGLLLAGREAGAAI
                     PRFAAWVQGLGVWGPLVFILGYAVATVAFVPGSLLTLAAGAIFGVVQGTLYTLAGATL
                     GASCAFLVARYVARRAIERRIAGNARFAAIDRAVSGEGFKIVALLRLSPAFPFNLLNY
                     ALGLTRVRFLAYLGASIAMLPGTLLYVYYGKAAGSLAAALGGGATAQRGPEFWITLGI
                     GLVATVLVTTLITRIAGRALKQQIGAPEEPHND"
     gene            2309..3826
                     /locus_tag="VEL74_03740"
     CDS             2309..3826
                     /locus_tag="VEL74_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HYG61669.1"
                     /translation="MIEASPVEPLDEHNRTLIDNVHPPAWVNPEPAGRYHLVVIGAGT
                     AGLVSAAGAAGLGARVALVERHLMGGDCLNVGCVPSKAVLRAARAWHDAREARARFGG
                     PAVEGDGDFGAAMERMRRLRAGISVHDSAKRFAGLGVDVFLGCGRFVSPSEVEVGGKR
                     LRFRRAVIATGGRAAVPPIPGLAEAGYLTNETIFNLTELPRRLVVIGGGPIGCELAQA
                     FARFGSRVTQLVQEGHVLPREDAGAAAVVQEALVRDGVQLELGMKLVEVRRQGGETVV
                     VFENGGERRQTVADQILLSVGRAPNVEGLGLEAAGVRYGKKGVEVDDHLRTSQSHIYA
                     CGDVSSRYPFTHIADAQARIVIQNALFFGRSKASALTVPWCTYTSPEIAHVGLYEKDA
                     REKGMEVDTLTVPLSSVDRAILEGATEGFLRVHLERGKDRILGATLVAEHAGDMIGEL
                     CLAITQGIGLGKIAGVIHPYPTQGEVVKKAADTWRRGKLTPGVKKLFERWFKVFG"
     gene            3858..4019
                     /locus_tag="VEL74_03745"
     CDS             3858..4019
                     /locus_tag="VEL74_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61670.1"
                     /translation="MRSTLLAALCVALFAPLLQAEPVQLADVDPLTLSIPDTEVLLPK
                     AAADIRPAD"
     gene            4287..5624
                     /locus_tag="VEL74_03750"
     CDS             4287..5624
                     /locus_tag="VEL74_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYG61671.1"
                     /translation="MALASLPEVQAVEEVRVFQGDRIEGGALIKATQLRNELGGTGRG
                     VGVAVIDSGIYASHPDFGSRVVAHLDATRTTGDGRIDQAGHGTAVAGIAAGAGGGMAP
                     EANLWAMKALNSAGQGTSDWILTALNAVYAARDDHGGIRVVNMSLSDATLNATDCDVL
                     SPAFSQAIDRLTAAGIAVFTSSGNSGYATGISHPACLSNAISVGALYDADSGPALFSV
                     CRDASTAADQIICYSNSGPPLDLLAPSHCARTTKAGGGYLNCFGGTSASSPYVAGVAA
                     QILSLRPDTTPAALREALMSTGRPVGEPWTGLVRHRIDAVAAYHRLTGGATPDLCVPS
                     ATTLCLAGGRFKVEAAYEAASGAAGQAHLVQLTDDSGYLWFFNSKNVEAVVKVLDACS
                     LGGKYWVFAGGLTDVRTTLNVTDTRTGTVKTYTNPQGAAFLPIQDTAAFATCP"
     gene            complement(5621..>6986)
                     /locus_tag="VEL74_03755"
     CDS             complement(5621..>6986)
                     /locus_tag="VEL74_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014528074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61672.1"
                     /translation="KDEILDGLARRGNVAQFVSFGPDLGQRYAWVRGLPPNQPFPSLA
                     EAVAALLAASPERSVNIRSYAPDDPKSREFLYGQTDAAAILGQLRRLAGEGLYTVVNE
                     TVDIHDGGVSGVAFGAALELAPGDTPRCVEKPGTASFPRDAGLRLLETVYRFRPALPE
                     RPELRVELSLHPLRRGYRHEHTILWETEKPGPAPTAPEISWPNRFSRFLGDKTFGLLV
                     AHTLGLPVPRTQVIPRGLAPFTFGGDTGLAETWIRTCPREQVPGFFTTRRGWVDPFRL
                     FEEEDPSGGEIAAVLCQQAVEARYSGALVARAEGGPVIEGVAGPGDDFMVGRRAPDEL
                     PDDVASAVRRTYERARGALGPVRFEWVYDGARVWILQLHHQRSGAGGPGEERVIVSGD
                     AERFHRLEVAEGIEALRQLIGRIQGSGDGIVLAGRVGVTSHFGDLLRRARIPSRIEEP
                     EGGP"
BASE COUNT          965 a         2580 c         2464 g          977 t
ORIGIN      
        1 caacccgccc ccctacaccc gtgtgctccg cccctgccgc ccgggcgacg gcatccgcca
       61 gatctcgacc gcggaggagc actggctgct cgcccgcttc gaaggggcgg cgcgggacgg
      121 gcggatcggc aagatggtgc ccgcctccgg cgcggcgagc cgcatgttca agtcgctgct
      181 cgcctacgtg aatggctcag gaggagagcc cgagggcgag gtccgcaccc tgttcgacaa
      241 cctggagcgc ttccccttct atgacgccct ggcggcggtc atggcgcgcg gcggccacaa
      301 cctgcggcag gcggtggacg gaggggacta caagacggtc ctcgagtacc tgctgacgcc
      361 ccgcgggctc ggctacgagg agctccccaa ggggctgctc ctcttccatc actaccccga
      421 gggtccgcgc acgccgttcg aggagcacct ggtcgaggcg gtggagcaga cgcggagcgc
      481 cgacggccgc tgccggctcc acttcaccgt ctcgccgcag cacgaggagg agttccgcag
      541 gctgctggcg gaggtcggcc cgcgctacga ggagcgctac ggcgtccgct tcgacgtcac
      601 gttctcctac cagaagcgct cgaccgacac gctggcggtc gatccggaga accgtccgtt
      661 ccggcaggac gacggcaccc tgctcttccg gcccggcggc cacggcgcgc tcctcgacaa
      721 cctggacgcg ctgggacgga acggctggga catcgtcctg atcaagaaca tcgacaacgt
      781 cgtgccggaa gagcgcaggc ccctgatctc gcagtggaag cggctgctga ccggccagct
      841 cctggcggtc caggaccgcg tctggcggct gctggagcgt ctggaggagg ggtcgacgaa
      901 cgccctcgac gaggcggcgg ccttcctgga gaaagagctg tcgcggtcgc tgccggcgga
      961 gctggcgggg gtgtccgaag aggtgcggcg gcggcggctg atcgacctgc tcgaccggcc
     1021 gctccgcgtc tgcggcatgg tgcgcaacct gggggagccc ggcggcggcc ccttctgggt
     1081 ggagtcgaaa accggcgagg tgtcgatcca gatcgtcgag acgtcgcagc tcgaccccaa
     1141 gtcggaggag cagcaggcgc agctcgcggc ctccacccac ttcagcccca ccgacctggt
     1201 ctgcgccctg cgcgaccgtc acggccggcc gtacgacctg cacgcctacg tcgatccgtc
     1261 gacggtcttc atgtccacca agtcgcacga ggggcggccg ctcaaggcgc tggagcgccc
     1321 gggcctgtgg aacggctcga tggcgggctg gagctccgtg ttcgtcgagg tgcccgacgc
     1381 cacgttcgcg ccggtgaaga ccgtcaccga cctgcttcgc cctgagcatc agggctgagc
     1441 ctcgtagccc ggcgactcat cgccgggcta cgaggcagcg ccgcccctcc ttccggtact
     1501 ccgacgcatc cattccggcc gccgtggacg tagagttcac gctgcgagag aggaggagag
     1561 atgagcgccg acgccaaagc cgccgccccc ggaggctccg ggagccgctg gaagcgggtc
     1621 gcctatgccg gaatcgccgt cgccgcggtc gcggggctgc tgctcgccgg ccgcgaggcg
     1681 ggcgccgcga tcccccgctt cgcggcctgg gtgcagggtc tgggcgtgtg gggccccctg
     1741 gtgttcatcc tcggctacgc ggtggcgacg gtggcgttcg tccccgggtc gctgctgacg
     1801 ctcgccgccg gcgcgatctt cggcgtggtc caggggacgc tctatacatt ggccggcgcg
     1861 accctggggg cttcgtgcgc gttcctggtg gcgcgctacg tggcgcggcg ggcgatcgag
     1921 cggcggatcg ccggcaacgc ccgcttcgcc gccatcgacc gggcggtgtc gggcgagggg
     1981 ttcaagatcg tggcgctgtt gcggctctcc ccggccttcc ccttcaacct gctgaactac
     2041 gcgctggggc tcacccgggt ccgctttctc gcctatctcg gggcgtccat cgccatgctc
     2101 ccggggacgc tcctctacgt ctactacggc aaggcggccg gcagtctggc cgccgcgctg
     2161 ggcggcggcg ccacggccca gagggggccg gaattctgga tcaccctggg gatcggcctg
     2221 gtggcgacgg tgctggtgac caccctcatc acccgcatcg cgggcagggc cctgaaacag
     2281 cagatcggcg cgccggagga gccacacaat gattgaggcc tcccctgtcg agccgctgga
     2341 cgagcacaac cggacgctga tcgacaacgt ccatccgccc gcctgggtga accccgagcc
     2401 cgccggccgc taccacctgg tggtgatcgg cgccggcacc gccggcctgg tctcggcggc
     2461 gggggcggcg gggctcggcg cccgggtcgc cctcgtcgag cgccatctca tgggcggcga
     2521 ctgcctgaac gtcggctgcg tgccgtccaa ggcggtcctc cgcgccgccc gcgcctggca
     2581 cgacgcgcgc gaggccaggg cgcgcttcgg cggcccggcg gtggagggcg acggcgactt
     2641 cggcgccgcc atggagagga tgcgccggct gcgcgccggc atcagcgtcc atgacagcgc
     2701 caagcgcttc gccggcctgg gggtggatgt cttcctgggc tgcggccgct tcgtctcgcc
     2761 atccgaggtg gaggtcggag ggaagcgcct gcgcttccgg cgggcggtca tcgccaccgg
     2821 cggccgggcg gcggtgcccc ccatcccggg gctcgccgag gcgggctacc tcaccaacga
     2881 gacgatcttc aacctgacgg agctcccccg ccgcctcgtg gtgatcggcg gcggccccat
     2941 cggctgcgag ctggcgcagg cgttcgcccg cttcggcagc cgggtcaccc aactcgtcca
     3001 ggaggggcac gtgctcccgc gcgaagacgc cggcgccgcg gccgtcgtcc aggaggccct
     3061 ggtccgggac ggcgttcagc tcgagctcgg gatgaagctg gtggaggtgc ggcgccaggg
     3121 gggcgagacc gtcgtggtct tcgagaacgg cggcgagcgc cggcagacgg tagcggacca
     3181 gatcctgctc tccgtgggcc gggcccccaa cgtcgagggg ctggggctgg aggcggccgg
     3241 cgtacgctac ggcaagaagg gcgtcgaggt ggacgaccac ctgcgcacca gccagtccca
     3301 catctatgcc tgcggcgacg tctcctcgcg ctacccgttc acccacatcg ccgacgccca
     3361 ggcgcgcatc gtcatccaga acgccctctt cttcggccgg tccaaggcga gcgccctgac
     3421 cgtcccctgg tgcacctaca ccagccccga gatcgcccac gtcggcctct atgagaagga
     3481 cgcccgcgag aaggggatgg aggtcgacac cctgaccgtc cccctgtcgt cggtggaccg
     3541 cgccatcctg gagggggcca ccgaggggtt cctgcgggtg cacctggaga gggggaagga
     3601 ccgcatcctg ggcgctacgc tggtggccga gcacgccggc gacatgatcg gcgagctctg
     3661 cctggcaatc acccagggga tcggcctggg caagatcgcc ggcgtcatcc acccctaccc
     3721 cacccagggc gaggtggtga agaaggcggc cgacacctgg cgccgcggca agctgacgcc
     3781 gggggtcaag aagctgttcg agcggtggtt caaggtcttc gggtaacatg gatgctccaa
     3841 ccaggaaggg aacatccatg agaagtacgt tgctggccgc tctgtgtgtc gctcttttcg
     3901 cccccctgct ccaggccgag ccggtccaac tcgctgacgt cgatcctctc accttatcga
     3961 ttcccgacac cgaggtcctc ctgccgaagg ccgccgcgga catccgcccg gccgactaga
     4021 agatcacccc cggcctgttc gcccaggcgg ccacccaatc cctcgacaag gccggcgaca
     4081 cgccgctgga cgtcatcgtc ctgctccacg agccgccggc ggcacccgcc gggaccaagg
     4141 ctgccgggtc ctcccgggtc gagcgcacgg ttgcgatcgc cgccgtcgag cagcagctcg
     4201 tacgccgcgc gccggcgggc ttctcggccc ggcgcggcct gtcgcacctc cccatcgtct
     4261 tcggccaggt gcggcgggcg gacctgatgg cgctcgcctc actccccgag gtgcaggccg
     4321 tcgaggaggt gcgcgtcttc cagggcgacc ggatcgaggg cggggccctg atcaaggcca
     4381 cccagctccg caatgagctc ggcggcaccg gccgcggcgt gggcgtggcg gtgatcgact
     4441 ccgggatcta tgccagccat ccggacttcg gcagccgcgt cgtggcgcac ctcgacgcca
     4501 cccgcaccac gggggacggc aggatcgacc aggccgggca cggcaccgcg gtggccggca
     4561 tcgccgccgg cgccggcggc ggcatggcgc ccgaggcgaa cctctgggcg atgaaggcgc
     4621 tgaactcggc gggccagggg acgagcgact ggatcctgac cgcgctcaac gccgtctacg
     4681 cggcgcgcga cgaccacggc ggcatccgag tggtcaacat gtctctcagc gacgccaccc
     4741 tgaacgccac ggactgcgac gtcctgtcgc cggccttttc ccaggcgatc gatcgtctga
     4801 ccgcggccgg catcgccgtc ttcacctcgt cgggcaacag cggctatgcc accggcatct
     4861 ctcatccggc ctgcctctcc aacgccatct cggtcggcgc gctctatgac gccgacagcg
     4921 ggccggcgct cttctcggtc tgccgggacg cgtcgacggc ggcggaccag atcatctgct
     4981 actccaactc gggaccgccg ctcgacctcc tggctccctc gcactgcgcc cgcaccacca
     5041 aggcgggcgg cgggtacctc aactgcttcg gcgggacctc ggcgtcttcc ccttacgtgg
     5101 ccggcgtcgc cgcccagatc ctctccctgc gcccggacac cacgccggcc gccctgcgcg
     5161 aagccctcat gagcaccggg cggccggtcg gcgagccctg gaccgggctc gtccgccacc
     5221 ggatcgacgc cgtcgccgcc taccatcgcc tcaccggcgg cgccacgccc gacctctgcg
     5281 tcccgagcgc caccaccctg tgtctcgcgg gtggacgctt caaggtggag gcggcctatg
     5341 aggccgcctc cggcgccgcg gggcaggccc atctcgtcca gctcaccgac gactcgggct
     5401 atctctggtt cttcaactcc aagaacgtcg aagccgtggt caaggtgctg gacgcctgct
     5461 cactcggagg caagtactgg gtcttcgccg gcggcctgac ggacgtgcgg acgaccttga
     5521 acgtcaccga cacccggacc gggacggtga agacctacac caacccgcag ggcgccgcct
     5581 tcctgccgat ccaggacacg gccgccttcg ccacctgccc ctagggcccg ccctccggct
     5641 cctcgatccg cgacgggatg cgcgcccgcc ggaggaggtc gccgaagtgg ctggtaaccc
     5701 cgacgcggcc ggcgaggacg atgccgtcgc cgctcccctg gatccggccg atgagttggc
     5761 ggagggcttc gatcccctcc gccacctcca gccggtggaa gcgctctgcg tcgccggaca
     5821 cgatgacgcg ctcctccccc gggccccccg ctccggatcg ttgatggtgg agctggagga
     5881 tccagacccg tgcgccgtcg tagacccact cgaagcgcac cggcccgagg gctccccggg
     5941 cccgctcgta cgtccggcgg acggccgagg cgacgtcgtc cggcagctcg tcgggcgcgc
     6001 gcctgcccac catgaagtcg tcgcccgggc cggccacccc ctcgatcacg gggccgccct
     6061 cggcccgcgc caccagcgcc cccgagtagc gcgcctccac cgcctgctgg cagagcaccg
     6121 cggcgatctc cccgccggac gggtcctcct cttcgaacag acggaagggg tcgacccagc
     6181 cgcggcgggt ggtgaagaag cccggcacct gctcccgcgg gcaggtgcgg atccaggtct
     6241 cggcgaggcc cgtgtcgccg ccgaaggtga acggggcgag cccgcggggg atcacctggg
     6301 tacgcggcac ggggagcccc agggtgtggg ccaccagcag gccgaaggtc ttgtcgccga
     6361 ggaagcggct gaagcggttc ggccaggaga tctcgggcgc ggtcggcgcc ggccccggct
     6421 tctcggtctc ccagaggatg gtgtgctcgt gacggtaccc tcgccgcaac gggtgcaggc
     6481 tgagctccac ccgcagctcc gggcgctccg gcagggccgg ccggaaccgg tagacggttt
     6541 ccagcagccg caggccggcg tcgcgcggga acgacgccgt gcccggcttc tcgacgcagc
     6601 gcggcgtgtc cccgggcgcc agctccagcg cggcgccgaa agccaccccg gaaacgccgc
     6661 cgtcgtggat gtccacggtc tcgttgacga cggtgtagag cccctccccc gccagccgcc
     6721 ggagctgccc gaggatggcg gccgcgtccg tctggccgta gaggaactcc cggctcttgg
     6781 ggtcgtccgg cgcgtagctg cggatgttga ccgaccgctc gggcgaggcg gcgagcagcg
     6841 ccgcgacggc ctcggccagc gacgggaagg gctggttggg cgggagaccc cggacccagg
     6901 cgtaccgctg cccgaggtcc ggcccgaagc tcacgaactg ggcgacgttg ccccggcggg
     6961 caagcccgtc gaggatctcg tccttg
//
LOCUS       DASPJI010000138         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_810203, whole genome shotgun sequence.
ACCESSION   DASPJI010000138 DASPJI010000000
VERSION     DASPJI010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810203"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VEL74_03760"
     CDS             complement(<1..420)
                     /locus_tag="VEL74_03760"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HYG61673.1"
                     /translation="MKKALLVLPLLLLAAVTARAAAPPDYSAWQSLLSKYYDPARGMN
                     YKALKANDAAALKQLRGRMSQVDVKALSPKDQLAYWINLYNISVVGIVVDEYPVESIR
                     DISTDPITRLNVFKKPVVAVRGGALSLDDIENVKVREG"
     gene            523..732
                     /locus_tag="VEL74_03765"
     CDS             523..732
                     /locus_tag="VEL74_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61674.1"
                     /translation="MHKKAKKLSLSRETVRTLDQSFLPNIAGADTAFQTNCGTCTNPS
                     YCRRCLPSPTFLYGCETNTCDCPEL"
     gene            complement(776..>2183)
                     /locus_tag="VEL74_03770"
     CDS             complement(776..>2183)
                     /locus_tag="VEL74_03770"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HYG61675.1"
                     /translation="AAAVPEKPNRYQSKKGAQDAHEAIRPTLFDLPPDSVRDFLKPDE
                     WKLYKLIWDRFIASQMLPAVFDVTQADIENGPYVLRATGRVMKSPGFLAVYRETPDED
                     APADAGAGGADGEEAAEGMLPPLTEGQALNLVKLLSEQKFTQPPAQYSEATLVKALEE
                     NGIGRPSTYATILSTLTDRDYVDKAEGRFRPSPLGSLVNGMLQKGFHDILNEGYTAAL
                     EEQLDRIEDGEVAWKQAVADFDEKFSQDLETAGEQMPNVKKEGVPTGETCPNCGSPLL
                     MRFGRYGTFIGCSNYPDCKYTRDMAAPAAKSDAPAAANGEAVTAEGAAAAGATAEEEI
                     PPCEVCGKPMQIRRSRYGTFYGCTGYPDCKNLRKIGPKPQAPKPTGVTCPECGKGEMQ
                     EKSSRRGKTFWSCSRYPDCKAALWKKPVLRPCPDCGASFLLEKVTKRHGTQYICNTEG
                     CGHIEDAAPDETAEAVSA"
BASE COUNT          309 a          737 c          760 g          377 t
ORIGIN      
        1 cccctcccgg accttgacgt tctcgatgtc gtccagcgac agcgcccccc cgcgcaccgc
       61 gaccaccggc ttcttgaaga cgttgagccg cgtgatgggg tcggtcgaga tgtcgcggat
      121 cgactccacc gggtactcgt cgacgacgat gcccaccacc gagatgttgt agaggttgat
      181 ccagtaggcg agctggtcct tgggggacag cgccttgacg tccacctggg acatgcggcc
      241 gcgcaactgc ttcagggcgg ccgcgtcgtt ggccttcagc gccttgtagt tcatccccct
      301 ggccgggtcg tagtacttgg agagcaggct ctgccacgcc gagtaatcgg gcggggcggc
      361 ggcccgggcc gtcacggcag cgagcaggag cagggggagg acgagcaggg ccttcttcat
      421 gagagcgatg gtagcgcagg cggatcacca gacccaccgg cgcgcactct ccaggatagg
      481 gccgccactc ggcgcccacg atccgatcct ggagaccaga gaatgcacaa gaaggcgaag
      541 aagctgtcgc tgagcaggga gaccgtccgc accctcgacc agtcgttcct tcccaacatc
      601 gccggcgccg acacggcgtt ccagacgaac tgcggcacct gcaccaaccc gagctactgc
      661 cggcggtgcc tgccgtcgcc caccttcctg tacgggtgtg aaaccaacac ctgcgactgc
      721 ccggagctgt aaaagaccgc agggtgcgcc gtgagcacgg cgcaccccgc gtgttctacg
      781 ccgacaccgc ttcggccgtc tcgtccggcg cggcgtcctc gatgtggccg cacccctcgg
      841 tgttgcagat gtactgcgtg ccgtggcgct tggtgacctt ctcgaggagg aagctggcgc
      901 cgcagtccgg gcagggacgg aggaccggct tcttccacag ggcggccttg cagtccgggt
      961 agcggctgca ggaccagaag gtcttgccgc ggcgggagct cttctcctgc atctccccct
     1021 tgccgcactc cggacaggtg acgccggtcg gcttcggagc ttgcggcttg gggccgatct
     1081 tgcgcaggtt cttgcagtcc ggatagccgg tgcagccgta gaaggtgccg tagcggctgc
     1141 ggcggatctg catcggcttg ccgcagacct cgcagggggg gatctcctcc tcggccgtgg
     1201 cgcccgccgc cgcggcgccc tcggcggtga cggcctcgcc gttcgcggcg gccggggcgt
     1261 cggatttcgc cgccggggcc gccatatcgc gggtgtactt gcagtccggg tagttgctgc
     1321 agccgatgaa cgtgccgtaa cggccgaagc gcatgaggag aggcgagccg cagttcgggc
     1381 aggtctcgcc ggtgggcacg ccctccttct tgacgttggg catctgttcg cccgccgtct
     1441 cgaggtcctg cgagaacttc tcgtcgaagt cggccaccgc ctgcttccag gcgacctcgc
     1501 cgtcctcgat gcggtcgagc tgctcctcga gcgccgcggt gtacccctcg ttgaggatgt
     1561 cgtggaaccc cttctggagc atgccgttga ccagcgagcc caggggggac ggacggaagc
     1621 gcccctcggc cttgtcgaca tagtcgcggt cggtcagcgt cgacaggatc gtggcatagg
     1681 tcgacggccg gccgatgccg ttctcctcca gcgccttgac cagcgtcgcc tcgctgtact
     1741 gcgccggcgg ctgggtgaac ttctgttcgg agagcagctt gacgaggttc agcgcctgtc
     1801 cctcggtcag cggcggcagc atcccctcgg cggcctcctc gccgtcggcg ccaccggctc
     1861 cggcatcggc gggggcgtcc tcgtccggcg tctcgcggta gaccgccagg aagccggggc
     1921 tcttcatcac ccggccggtg gcgcgcagga cgtaggggcc gttctcgatg tccgcctggg
     1981 tgacgtcgaa gaccgccggc agcatctggg aggcgatgaa gcggtcccag atcagcttgt
     2041 agagcttcca ctcgtcgggc ttcaggaagt cgcgcaccga gtccggcggc aggtcgaaga
     2101 gggtggggcg gatcgcctcg tgggcgtcct gggccccctt cttcgactgg tagcggttcg
     2161 gcttctcggg caccgcggcg gcg
//
LOCUS       DASPJI010000139         8402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_753851, whole genome shotgun sequence.
ACCESSION   DASPJI010000139 DASPJI010000000
VERSION     DASPJI010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8402)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8402
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753851"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VEL74_03775"
     CDS             complement(<1..232)
                     /locus_tag="VEL74_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYG61676.1"
                     /translation="MPDPQRKIFVLDTSVILHDFNAILSFQEHDVAIPITVLEELDQF
                     KKGNSVLNLQAREFIRQLDRLSSHNMLQEWIPL"
     gene            complement(342..1250)
                     /locus_tag="VEL74_03780"
     CDS             complement(342..1250)
                     /locus_tag="VEL74_03780"
                     /inference="COORDINATES: protein motif:HMM:NF025697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HYG61677.1"
                     /translation="MKKIGTLVTSSLVVLLVALAPAPATPQSAPTAPMGYSVQSDSNT
                     DVLYAINLATGAAAPLGPTGFGDVESLAFNPDCSTLYAVDDVKDRLITCSTATGACTA
                     VGALGVDVTDTGLAFTSNGGLFMSTDAPKNPSNFYRLDPATGRATLIGVQGQEVTGLA
                     ANRNGLFGLGGDGRNNLVRIDPRTGRATQVGSLGAVATPDGGIDFDARGVLYGITDGG
                     STPNSPSQIFTVNPVTGAAQVVANVTSGGQTINGFEGLAIDDGICSVLIPKAAAIDAP
                     TASEWALGSLAIALAFAGVFVLRRLF"
     gene            1328..2239
                     /locus_tag="VEL74_03785"
     CDS             1328..2239
                     /locus_tag="VEL74_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYG61678.1"
                     /translation="METREASATAGDSSEGGALASRVRLLAAAGLFSLAGALVKGVSL
                     DGWQVASFRSAVAALVLFALLPEARRRPPGRVVAAASVYSATMITFILATKLTTAASA
                     IFLMAAAPLYVLLLGPWLLRERTTLPDILYMVALAAGLGALFVEMDPASATAPDPRLG
                     NLLAVLSGVCWALTLISLRGLSRQREGGAIDWGPAASVWGNVITFAVCLPLALPVEGG
                     SPSDWLGVAVLGVFQLGLGYFFLVRGLRRVPALEASLLLLLESVLNPVWAWLVHGERP
                     GGWTLAGGSLILLATVARTGWDARRGL"
     gene            complement(2236..3930)
                     /locus_tag="VEL74_03790"
     CDS             complement(2236..3930)
                     /locus_tag="VEL74_03790"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HYG61679.1"
                     /translation="MPILLAALTVLAACRGDEPMPPEVSQILRQTVETQTLPASLKDQ
                     KEQVRAWEEMRRFYAARQFQPAWSDSRGPLPEARELLAAIDQLSAQGIDPRRYRKDEL
                     AALLPEKGWKDLADDPREQRRVADLDAQLTFTFLTIATHTAVGRLQPDTVRTDWYTKP
                     RNLDVDGLLNRALQSQEEGAIQKALRGMTPPGEEYARLSQVLDRYQQIAEKGGWPSIP
                     AGPELKPGDGGPRVAALRARLAAEGDLAANAAAGAAFDEPLAQAVSRFQRRNGLEVTG
                     KVDEATLAALNVPAEDRLRQLVVNMERWRWMPSDLGERYIWVNIPEFRMDLFEGDRPA
                     VSMRVVVGKEQSRTPVFSDKMEYLELNPFWNLPDSIINEEVLPALAKNPDYLASHEME
                     VVQGWEADAPAVSSYDPSQLGKPGSEYRLRQRPGAANPLGKVKFMFPNRFDIYLHDTP
                     ADHLFDRAERDFSHGCIRLERPIELAQYLLKDDPKWTPEAIQAKLASGETTSVPLPRP
                     LAVHLVYFTAWADENGVVHFRDDVYGHDAKLAAALMEEPPVPLDLPAIRGDVRAAR"
     gene            complement(4060..6888)
                     /gene="ligD"
                     /locus_tag="VEL74_03795"
     CDS             complement(4060..6888)
                     /gene="ligD"
                     /locus_tag="VEL74_03795"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HYG61680.1"
                     /translation="MALEEYKRKRDFRKTPEPAGKPVARKGKGKGQEAGGLSFVIQKH
                     AATRLHYDFRLEMEGVLRSWAVPKGPSYDPAEKRLAVHVEDHPMEYGTFEGIIPKGQY
                     GGGTVLLWDQGTWYPEGDPVAAYRKGHLKFRLEGEKLHGGWHLVRLGGRRSGNDDEKE
                     NWLLFKENDETALPGSTDAITRERPESVTTGLSLEEIAANPERVWQSNRAEKNASFKE
                     KLQATVRASRAAKKAPKPEAAPVKVPGARKGAFPAKLEAQLATLVDEVPRGEEWIHEL
                     KFDGYRVLCEVRGGKVRLVTRNGKDWTDRFTPVAKAAGALPVRDALLDGEVVVLEKDG
                     TTSFQALQNALSGQRDDDLVYFAFDLLYLDGHDLRRAELRERKRALADLLAAVPSGPK
                     GRIRYSDHVEGSGEDFYRQACSFSLEGVISKRGDLPYHPGRNKDWLKIKCLKRQELVV
                     AGFTEPEGARVGLGALLLGVYEGDGLVFAGKVGTGFNDRLLRDLRKRLGRLETPDSPF
                     AGPPRDLVRAAARGDVHWVKPELVGEVAFTEWTRDGVLRHPTFQGLREDKSPQEVRRE
                     LPQALPPEAGGEDEPAEPARSARTARPAAKTAAKKPAAKKAPAKKPAAKKAPAKEAAP
                     EKPAPAAPAAPAARSGKGAKETEVAGVRLSNPDKVLFPEQGLTKRDLALYYERIAGWI
                     LPHIKDRPLTLVRCPEGQGKECFYQKHVSEHVPKSVVRVTITGDPQPYGAVSSVQGLI
                     ALVQMGVLELHIWGSHRDDIEKPDYVVFDLDPDEGLDWQRVAEAALLVRQRLTDLGLE
                     SFLKTTGGKGLHVVVPLTRRKGQEWDEVKAFTKAVAEDIVAANPGRYTSMLPKARRQG
                     RIFIDYLRNGRGATSICAFSTRARVGAPVSVPLRWDELLDPELRGNTYNVRTLPERLD
                     SLKEDPWEGFHKVRQSITAAIKKAVGMT"
     gene            complement(6903..7841)
                     /locus_tag="VEL74_03800"
     CDS             complement(6903..7841)
                     /locus_tag="VEL74_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HYG61681.1"
                     /translation="MPRSIWKGSISFGLVNIPVGLYSAESREEISFHLLDKRNMARVR
                     YKRVNEQTGKEVPWEETVRGYEFESGQYVIMTDEDLKRANPESTQTVDIVDFVDLDDI
                     SPTYFDKPYYLAPDKKGRRSYALLRETLKRTRKVGIAKVVIRTKQYLAAVMAQDDVIL
                     LEILRFAHELRDPGELDLPSGNEGVSERELDMAERLVEGMVDSWKPERYKDDYQKDLM
                     ALIKERVKAGQLESPPASDEEEKELRPARGQVVDLMALLKQSVDQRGKGGHAADKDEK
                     PKKAAAKKAPAKAARKPAARRTATKSTSGSRKKKSA"
     gene            7964..>8402
                     /locus_tag="VEL74_03805"
     CDS             7964..>8402
                     /locus_tag="VEL74_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HYG61682.1"
                     /translation="MGSKTRTQRWKVEAGRAEPAPDDLATEEPLEIRLLFRGESRVVA
                     VTMRTPGADAELAAGFLFAEGVVDRPGRIRGFTFPEPRRGVLSLADQAGEHASNVIEV
                     ELDGEGPEPELAPLDRHFFATSACGVCGKAGLESLRLRVPTELP"
BASE COUNT         1078 a         2835 c         3053 g         1436 t
ORIGIN      
        1 ccagcgggat ccactcctgc agcatgttgt ggctcgacag ccggtcgagc tggcggatga
       61 actcccgggc ctggaggttg aggacggagt tgcccttctt gaactggtcc agctcctcca
      121 gcacggtgat cgggatcgcg acgtcgtgct cctggaagct caggatcgcg ttgaagtcgt
      181 ggaggatgac ggaggtgtcg aggacgaaga tcttgcgctg gggatcgggc atggtcggtc
      241 gtacggctcc cggaggaagg ttggctccac gcggactccc tgaggctagc tcaggagcct
      301 ggcgagggca aggcggcccc gttggggagc cggaccgtcc tctagaacag gcggcgcagc
      361 acgaagacgc ccgcgaacgc gagggcgatg gcgagggagc cgagggccca ctcgctggcg
      421 gtgggggcgt cgatcgccgc cgccttgggg atgaggacgc tgcagatgcc gtcgtcgatc
      481 gccagcccct cgaagccgtt gatcgtctgc ccccccgagg tgacgttggc gaccacctgc
      541 gcggcgccgg tcaccgggtt gacggtgaag atctggctgg ggctgttggg ggtggagccg
      601 ccgtcggtga tgccgtagag cactccgcgg gcgtcgaagt cgatcccgcc gtccggggtg
      661 gcgacggcgc ccagggagcc gacctgcgtc gccctgccgg tccgcgggtc gattcgcacc
      721 aggttgttcc tgccgtcgcc gcccaggccg aagaggccgt tgcggttggc ggcgaggccg
      781 gtgacctcct gcccctggac gccgatcagg gtggccctgc ccgtcgccgg gtcgagccgg
      841 tagaagttgg acgggttctt gggcgcgtcc gtcgacatga agaggccgcc gttggaggtg
      901 aaggcgaggc cggtgtcggt gacgtcgacc ccaagggcac ccacggcggt gcaggcgccg
      961 gtcgcggtgc tgcaggtgat cagccggtcc ttgacgtcgt ccaccgcgta gagggtgctg
     1021 cagtccgggt tgaaggccaa ggactcgacg tcgccgaagc cggtgggacc gaggggcgcg
     1081 gcggctccgg tcgccaggtt gatggcatag aggacgtcgg tgttgctgtc cgactggacg
     1141 gagtacccca tcggagcggt cggggcggac tgcggcgtgg ccggcgcggg ggcgagggcc
     1201 acgaggagga cgacgaggct cgaggtcaca agggtgccga tcttcttcaa ctgcgttctc
     1261 ccggcggatg cgcgtgctgg tgctccaagc ctactacaga tccctccgcg ccatagaatg
     1321 cgccgccatg gagacgcgcg aagcttcggc gacggcaggt gacagcagtg aggggggagc
     1381 gctggcctcg cgggtgcggc tgctcgcggc ggccgggctc ttctccctgg ccggggcgct
     1441 ggtcaagggg gtgagcctcg acggctggca ggtggcgagc ttccgctccg cggtggcggc
     1501 gctggtgctc ttcgcgctgc tccccgaggc gcggcgccgg ccgccggggc gggtcgtggc
     1561 cgcggcgtcg gtctactccg cgaccatgat caccttcatc ctcgccacca agctcaccac
     1621 ggcggcgagc gccatcttcc tgatggccgc ggcgccgctc tacgtcctgc tgctggggcc
     1681 atggctgctg cgcgagcgga cgaccctgcc ggacatcctc tatatggtgg cgctggccgc
     1741 gggcctgggg gccctgttcg tcgagatgga tccggcctcg gcgacggcgc cggacccgcg
     1801 gctcggcaac ctgctggcgg tgctgtccgg cgtctgctgg gccttgaccc tcatcagcct
     1861 gcgcgggctg agccggcagc gggagggcgg cgcgatcgac tggggaccgg ccgcgagcgt
     1921 gtggggcaac gtcatcacct tcgcggtctg cctgcccctg gccctgccgg tggagggggg
     1981 gagcccctcc gactggctcg gcgtcgccgt gctgggcgtc ttccagctgg gcctgggcta
     2041 tttcttcctg gtccgcggcc tgcggcgcgt gccggccctg gaggcgtcgc tgttgctcct
     2101 gctggagtcg gtgctcaacc cggtctgggc ctggctggtc cacggcgagc gccccggggg
     2161 ttggacgctc gccggcggca gcctcatcct gctcgccacc gtggcccgga ccgggtggga
     2221 cgcccgccgg ggactctagc gcgccgcccg cacgtctccc cggatcgccg gcaggtccag
     2281 cggcaccggg ggctcctcca tgagcgccgc cgccagcttg gcgtcgtggc cgtagacgtc
     2341 gtcccggaag tgcaccaccc cgttctcgtc cgcccaggcg gtgaagtaga cgaggtggac
     2401 ggcgagcggc cgcggcagcg gcaccgaggt ggtctcaccc gaggcgagct tggcctggat
     2461 ggcctccggc gtccacttag ggtcgtcctt gagcaggtac tgggccagct cgatgggccg
     2521 ctccaggcgg atgcagccgt ggctgaagtc gcgctcggca cggtcgaaca ggtggtcggc
     2581 gggggtgtcg tggaggtaga tgtcgaaccg gttggggaac atgaacttca ccttccccag
     2641 ggggttcgcc gcgcccgggc gctggcggag gcgatactcc gagccgggct tgccgagctg
     2701 cgatgggtcg taggagctca ccgccggggc gtcggcctcc cacccctgaa ccacctccat
     2761 ctcgtggctg gcgaggtagt ccgggttctt ggccagcgcc ggcagcacct cttcattgat
     2821 gatcgagtcc ggcaggttcc agaacgggtt cagctcgagg tactccatct tgtcgctgaa
     2881 caccggcgtg cggctctgct ccttgcccac caccacccgc atgctcaccg ccgggcggtc
     2941 gccctcgaac aggtccatgc ggaactcggg gatgttgacc cagatgtaac gctcccccag
     3001 gtcgctgggc atccagcgcc agcgctccat gttgaccacg agctggcgga gccggtcctc
     3061 cgccggcacg ttcagggcgg cgagggtggc ctcgtccacc ttgccggtga cctccaggcc
     3121 gttgcgccgt tggaagcggg agacggcctg ggccagcggc tcgtcgaagg cggcgcccgc
     3181 ggcggcattg gcggcgagat ccccctcggc ggcgagacgg gcgcggaggg cggcgacccg
     3241 cggaccgccg tcgcccggct tcagctccgg accggcgggg atggagggcc atccaccctt
     3301 ttcggcgatc tgctggtagc ggtccagcac ctggctcagc cgggcgtact cctcacccgg
     3361 tggggtcata ccgcgcagcg ccttctggat ggcgccctcc tcctgggact ggagggcccg
     3421 gttcagcagg ccgtccacgt cgaggttgcg cggcttggtg taccagtcgg tgcggacggt
     3481 gtcgggctgc aggcgcccga cggccgtgtg ggtggcgatg gtgaggaagg tgaaggtgag
     3541 ctgggcgtcc aggtcggcca cccggcgctg ctcccgcggg tcgtccgcga gatccttcca
     3601 tcccttctcg ggcagcaggg cggcgagctc gtccttgcgg tagcggcgcg gatcgatgcc
     3661 ctgcgccgag agctggtcga tggcggcgag aagctcccgt gcctcgggaa gagggccccg
     3721 gctgtcggac caggcgggct ggaactggcg cgcggcatag aagcggcgca tctcctccca
     3781 ggcgcgcacc tgttccttct gatctttgag cgacgccggc agggtctggg tctccaccgt
     3841 ctggcgcagg atctggctga cctcgggcgg catcggctcg tccccccggc aggcggcgag
     3901 gaccgtcagg gcggcgagaa ggatcggcat ggaatatcgg acaagaatag gcttcatcat
     3961 gggctcctcg gacggccgtc cagctcccgg agggggcctc cgccgtccgc tatgtttcaa
     4021 acagctcgga tagggctcgg gatttcgtgc gcggagcggc tatgtcatgc ctacagcctt
     4081 ctttatcgcc gccgtgatgg actggcggac cttgtggaaa ccctcccagg ggtcttcctt
     4141 caggctgtcc aggcgctccg ggagggtccg cacgttgtag gtgttgccgc gcagctccgg
     4201 gtccagcagc tcgtcccagc gcaggggcac cgagaccgga gcgccgacgc gagcgcgggt
     4261 ggagaaggcg cagatggagg tggcgccgcg gccgttcctc aggtaatcga tgaagatccg
     4321 cccctggcgg cgggccttgg ggagcatgga ggtgtagcgt cccgggttgg ccgccacgat
     4381 gtcctcggcc accgccttgg tgaaggcctt cacctcgtcc cactcctggc ccttgcgccg
     4441 ggtcagcggc acgaccacgt gcagcccctt gccgccggtg gtcttgagaa agctctcgag
     4501 gcccaggtcg gtcaggcgct ggcgcaccag gagggcggcc tcggccaccc gctgccagtc
     4561 gagcccctcg tccgggtcga ggtcgaagac cacgtagtcg ggcttctcga tgtcgtcgcg
     4621 gtgcgagccc cagatgtgga gctcgaggac gcccatctgc accaaggcga tgagcccctg
     4681 gacggagctg acggcaccgt acggctgagg gtcgccggtg atggtgacgc ggacgaccga
     4741 cttcggcacg tgctcgctga cgtgcttctg gtagaagcac tccttgccct gcccctcggg
     4801 gcagcgcacc agggtcagcg ggcggtcctt gatatgagga aggatccagc cggcgatccg
     4861 ctcgtagtag agcgccaggt cgcgcttggt gagcccctgc tcggggaaga gcaccttgtc
     4921 cggattggag agccggacgc cggcgacctc ggtctccttg gcccccttgc cgctacgggc
     4981 tgccggagcg gcgggagccg cgggagcggg cttctcgggg gcggcctcct ttgctggcgc
     5041 tttcttcgcc gccggcttct tggctggggc cttcttggcc gcgggcttct tcgccgccgt
     5101 cttggcggcg ggccgcgcgg tccgggcgga gcgggccggc tccgccggct cgtcctcgcc
     5161 gcctgcctcc ggcggcagcg cctggggcag ctcgcgccgc acctcctggg gggatttgtc
     5221 ctcccgcagc ccttggaagg tagggtggcg caggacgccg tcccgggtcc actcggtgaa
     5281 cgcgacctcg cccaccagct ccggcttgac ccagtggacg tcgccgcggg ccgccgcccg
     5341 taccaggtcc cgcggcgggc cggcgaaggg ggagtccggc gtctccagcc tgccaagacg
     5401 cttgcgcagg tcgcggagca ggcggtcgtt gaagccggtc cccaccttgc cggcgaagac
     5461 caggccgtcc ccctcgtaga caccgaggag cagggcgccg agccccaccc gggcgccctc
     5521 gggctcggtg aagccggcga cgacgagctc ctggcgcttc aggcacttga tcttgagcca
     5581 gtccttgttg cggccggggt ggtaggggag gtcgccgcgc ttggagatga ccccctccag
     5641 ggagaagctg caggcctggc gatagaagtc ctcccccgag ccctcgacgt gatcgctgta
     5701 gcggatgcgc cccttgggac cagagggcac cgccgccagg aggtccgcca aggcccgctt
     5761 gcgctcccgc agctccgccc gccgcaggtc gtggccatcc agatagagga ggtcgaaggc
     5821 gaagtagacc aggtcgtcgt cgcgctgccc cgagagggcg ttctggagcg cctgaaagct
     5881 ggtggtgccg tccttctcca ggaccaccac ctcgccgtcg aggagggcgt cgcgcaccgg
     5941 cagggcgccg gcggccttgg cgacgggggt gaagcggtcg gtccagtcct tgccgttacg
     6001 ggtgacgagg cgcaccttgc cgccgcgcac ctcgcagagc acgcggtagc cgtcgaactt
     6061 gagctcgtgg atccattcct cgccccgcgg aacctcgtcc accagcgtgg cgagctgggc
     6121 ctcgagcttg gccgggaagg cccccttgcg ggcgccgggg accttcaccg gcgccgcctc
     6181 aggcttggga gccttcttgg cggcacggct ggcacgaacg gtggcctgga gcttctcctt
     6241 gaaggaggcg ttcttctcgg cccggttgga ttgccagacg cgctccgggt tggcggcaat
     6301 ctcctccagg gaaagcccgg tggtgacgct ctccggccgc tcccgtgtga tggcatcggt
     6361 gctgcccggc agggccgttt cgtcgttctc cttgaagagg agccagttct ccttctcgtc
     6421 gtcgttgccg gaacgccggc cgcctaagcg caccaggtgc cagccgccgt ggagcttttc
     6481 cccctcgagc cggaacttga ggtgcccctt gcggtaggcc gccaccggat cgccctcggg
     6541 gtaccaggtg ccctggtccc agaggagcac cgtgccgccg ccgtattggc ccttggggat
     6601 gatcccctcg aaggtgccgt actccatggg gtggtcttcc acgtggacgg cgaggcgctt
     6661 ctccgcgggg tcgtagctgg ggcccttggg gaccgcccag ctccgcagca ccccttccat
     6721 ctccaaccgg aagtcgtagt gcagccgggt ggccgcgtgc ttctggatga cgaacgacag
     6781 cccgccggcc tcctggccct tgcctttgcc cttgcgggcg accggcttgc cggcgggctc
     6841 cggggtcttc cggaagtcgc gcttgcgctt gtattcctcg agggccacgg ctcagggtct
     6901 cctcaggcgc tcttcttctt ccgcgagcca ctggtgctct tggtggcggt gcgccgcgcc
     6961 gcgggtttgc gggcggcctt ggccggcgcc ttcttggccg cggccttctt cggcttctcg
     7021 tccttgtcgg cggcgtgacc gcccttgccc cgctgatcga cgctctgctt gagcagggcc
     7081 atgaggtcca cgacctggcc acgggccggc cgcagctcct tctcctcctc gtcagaggcc
     7141 ggcggcgatt caagctgccc cgccttgacc cgctccttga tgagggccat cagatccttc
     7201 tgatagtcgt ccttgtacct ctcgggcttc caggagtcga ccatgccctc gaccagccgc
     7261 tcggccatgt ccagctcgcg ctcgctgacc ccctcgttcc ccgagggcag gtccagctcc
     7321 cccgggtcgc gcagctcgtg ggcgaagcgg aggatttcca agaggatgac gtcgtcctgg
     7381 gccatcaccg cggccaggta ctgcttggtg cggatgacca ccttggcgat gcccaccttg
     7441 cgagtacgct tgagcgtctc ccgcaagagg gcatagctcc ggcgcccctt cttgtcgggc
     7501 gcgagatagt agggcttgtc gaagtaggtg gggctgatgt cgtcgaggtc cacgaagtcg
     7561 acgatgtcca ccgtctgggt cgactcgggg ttggcccgct tgaggtcctc gtcggtcatg
     7621 atcacgtact gccccgactc gaactcgtag ccccggacgg tctcctccca ggggacctcc
     7681 ttgcccgtct gctcgttgac ccgcttgtag cggacccgcg ccatgttgcg cttgtccagc
     7741 aggtggaagc tgatctcctc ccgcgattcg gccgagtaca ggccgacggg gatgttgacg
     7801 agtccgaagc tgatacttcc tttccagatc gatcgaggca tggccggtcc tccgctctcc
     7861 gctgggtcgg gtctcgcaaa ttgcatgccg caaactttta tggtccactg ccgctgggaa
     7921 gtccgcctga cggttgcgcg ggcgtttgtg agaagctcaa gtcatgggca gcaagaccag
     7981 gacgcagcgc tggaaggtcg aagcggggcg ggccgagccc gcgccggacg acctcgcgac
     8041 cgaggagccg ctggagatcc ggctgctgtt ccggggcgag agccgcgtcg tggccgtcac
     8101 catgcgtact cctggagccg atgccgaact ggccgccggc ttcctctttg ccgagggggt
     8161 ggtggaccgg cccgggcgga tccgcggctt caccttcccc gagccccgcc gcggcgtcct
     8221 ttccctggcc gaccaggcgg gggagcacgc ctccaacgtc atcgaggtcg agctggacgg
     8281 agagggaccg gagcccgagc tcgcccccct ggaccgccac ttcttcgcca ccagcgcctg
     8341 cggggtctgc ggcaaggccg ggctcgaatc gctgcgcctg cgggtgccta cggagctgcc
     8401 gc
//
LOCUS       DASPJI010000140         5050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_240239, whole genome shotgun sequence.
ACCESSION   DASPJI010000140 DASPJI010000000
VERSION     DASPJI010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240239"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VEL74_03810"
     CDS             complement(<1..364)
                     /locus_tag="VEL74_03810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG61683.1"
                     /translation="MTSNISAVVPTLGRSPLLVPCLEALRREGGADLEIVVVDQGPEP
                     VSLPQTLADRLVRPGGNLGFAGGTNAGIAAARGDLLALVNDDVLVEPGWLAALAGALE
                     ADPRAAAAQGVNLLLDDAH"
     gene            complement(374..2632)
                     /locus_tag="VEL74_03815"
     CDS             complement(374..2632)
                     /locus_tag="VEL74_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61684.1"
                     /translation="MKPWLSGERLRTAGVLAGSAVAAALLSRFLSLPADDPFRGTLAI
                     LPLLLAGFHLVRRQVESLGGRPLAAAGTAGELAALGGLVLLALARSPLGLARAGEAVA
                     AGLLLVLAVRLARQLAAARPLLGERLPRRPAAVFFLLPFLAYLAVLPWSARHRQPDGD
                     EPYYLLITHSLAYDLDADLTNNYAAGDWRHYMDRRIAPQPGDPVGPDGELYSRHNEML
                     PLVLAPAYRAAGKAGALVTMAALTAVLAWLTLRLARHYVPDGSGGPALAAYALVAFTP
                     PLLLYSYQVWVEVPAALLAAAALDRMLSLDGQRRWGWREWVGLGVPILLLPLLKIRFL
                     LVAAPLLALGWWHAGRPKKPMIILGLLLAAVAGGMLVHNLLLYANPLKIHTWEEVDPH
                     RYSPASYLKGGLGLFWDAAFGLFACAPVWLLLLAAVPLLLVRNRRLLLHLAVIALPYL
                     IVVAPRNEWYGGWSPPFRYALLALPLLGIALVPLLAAPLPAWRDRPGARALLAALGAL
                     TLVLTLVWVTVPGWTYNFADGRTYALDSLSGRLGLDLARLFPSSVRPRAATWLWPAVT
                     LVLVPLIWWLPARRSRVTAPQGPVGGGTAAALAGVAALLASVAVLPGAAARLPTRTVE
                     LEDPQVEKSGGHLHPDLWVIERTRYRGGWVLRVGERLEAPVNPGGRRVRLTLHAQFIR
                     NQPVPFTLDVRAGDRLLGSWTPRRERVWEAVELGPFDWPAGQPLTLSAHGPHPPGALN
                     GAVLDCVDLDWM"
     gene            complement(2629..4377)
                     /locus_tag="VEL74_03820"
     CDS             complement(2629..4377)
                     /locus_tag="VEL74_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61685.1"
                     /translation="MITTDLDAPSAPGADNSPAGRGPLLEELLRFLVPLAAFAATASF
                     AARELDPAGGAESAYLAVLAAAVLLAATALAPRPGVEAAAGSVLAAAAVWALPPGPGR
                     GAAVAVVLTATLAVAAARRLGGVLPDLPLPVAVPLSLSVQFLLRGELLFDAEASARTL
                     VALLALPVAGGIATAVLARHYGGHPALLAAGSAVLVGPGWNVLTTLALTSLAAGDLLA
                     RRDLSKTARVAAALVLLAPIAWKPWPGLAAATAGLALAWPVPALALPLGALAVWLLRP
                     GMALGEPRDAFLLVLLLPALLWIDRRQVRRLLVVLFLAASPWLLPERPALAAPLALAA
                     LLLRRDGPAAPLQKVWTGMLLLGTALLASYPWLREAPVLAVLGLLGLTTEPFLSWVPA
                     LAAAALAAFGLWRERRPEVRLLPSFLLAGLVFAVLLIRLPRPGTLLLPPETAVRLDAT
                     YPSRDAALPAPAHPVGSLVVESALANGAGLENGTPVAVFRLSDPAGRSVSWTLRAGEG
                     TGEWAARRPDVERTARLRSPEAWVSWVAGDFFAQRFRSRWDLDRPGRFTRLRVERAPG
                     LPPEVSLAVHQVEVRP"
     gene            4587..>5050
                     /locus_tag="VEL74_03825"
     CDS             4587..>5050
                     /locus_tag="VEL74_03825"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF014458.4"
                     /note="catalyzes a two-step reaction, first charging a
                     serine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HYG61686.1"
                     /translation="MLSRDLLRDDFETVRQTLATRNMDPSLLERWQRLDGERRAALVE
                     TEEIKRQRNEASRAIGKIKQQGGDAAGQMAEVGRMKGRIEELEARLATVEQEVESIET
                     ALPNLPHESVPVGKDESDNRVERVVGEPRAFGFEPKAHWDLGPELGILDFERA"
BASE COUNT          791 a         1830 c         1966 g          463 t
ORIGIN      
        1 ggtgggcgtc gtccagcagc aggttcaccc cctgggcggc ggcggcccgg gggtccgctt
       61 ccagagcgcc ggccagagca gccagccagc cgggctccac caggacgtcg tcgttgacca
      121 gggcgaggag atccccccgg gcggcggcga tgccggcgtt ggtgccgccg gcgaagccga
      181 gattccctcc gggccggacg aggcggtcgg cgagtgtctg gggaagagag accggctcgg
      241 ggccctgatc gacgacgacg atctccaggt ccgctccgcc ctcccgacgc agagcctcga
      301 ggcaggggac gaggagcggg ctccgcccca gggtgggcac gacggcgctg atattggagg
      361 tcacggcgga ggctcacatc cagtccaggt cgacgcagtc caggacggcg ccgttcaagg
      421 cgcccggcgg gtggggacca tgggccgaga gggtgagcgg ctggccggcg ggccagtcga
      481 aggggccgag ctcgacggcc tcccagaccc tctcccggcg cggggtccag gagccgagca
      541 ggcggtcgcc ggcccgtacg tccagggtga agggcacggg ctggttgcgg atgaactgcg
      601 cgtgcagggt cagccgcacc cgccgccctc ccggattgac gggcgcctcc agccgctcgc
      661 cgacgcgcag cacccagccg ccccggtagc gcgtccgctc gatcacccag aggtcggggt
      721 ggagatggcc gccactcttc tccacctggg ggtcctccag ctcgaccgtc cgggtgggca
      781 ggcgcgccgc cgccccgggg aggaccgcca cggaggccag cagcgccgcg acgcccgcca
      841 gcgccgccgc cgtgccgccg cccaccggtc cctgcggtgc cgtcacgcgg gatcgccgcg
      901 ccggcagcca ccagatcaga gggacgagga cgagggtaac ggccggccag agccaggtag
      961 cggcgcgggg ccggaccgag ctgggaaaca gccgcgccag gtcgaggccg agccggccgc
     1021 tcaggctgtc cagcgcgtag gtccggccgt cggcgaagtt gtaggtccac cccgggaccg
     1081 tcacccagac cagggtcaga accagggtga gggcgccgag ggcggcgagc agcgcccgcg
     1141 ccccgggccg gtcgcgccac gccggcagcg gcgccgccag cagtggcacc agcgcgatcc
     1201 ccaggagcgg cagcgccagg agcgcgtacc ggaagggggg agaccacccg ccgtaccact
     1261 cgttgcgcgg cgccacgacg atcagatagg ggagggcgat gaccgccagg tggagcagca
     1321 gcctgcggtt ccgcaccagg aggagcggca ccgcggcgag caggaggagc cagaccggcg
     1381 cgcaggcgaa gaggccgaac gccgcgtccc agaacaggcc gaggcccccc ttcagatagg
     1441 aagcggggct gtagcggtgg gggtcgacct cctcccaggt gtggatcttg agcgggttgg
     1501 catagaggag caggttgtgg accagcatgc cccccgcgac cgccgccagc agcaggccca
     1561 ggatgatcat cggctttttc ggccggcccg cgtgccacca gccgagggcc aggagcggcg
     1621 cggccacgag caggaagcgg atcttgagca gcggcagcag caggatcggc accccgagcc
     1681 ccacccactc cctccagccc cagcgccgct ggccgtccag ggagagcatg cggtccaggg
     1741 ccgccgccgc cagcagcgcc gccggcacct ccacccagac ctggtaggag tacaggagga
     1801 gcggcggggt gaaggcgacc agcgcatagg cggcgagcgc cggccccccg gagccgtccg
     1861 gcacatagtg gcgggcgagg cgcagcgtca gccaggccag gacggcggtg agcgcggcca
     1921 tggtgaccag ggcgccggcc ttgcccgccg cccggtacgc gggcgccagc accaggggga
     1981 gcatctcgtt gtgccgcgag tacagctcgc cgtccggccc caccggatcg cccggctgcg
     2041 gcgcgatgcg gcggtccata tagtggcgcc agtcgccggc cgcgtagttg ttggtgaggt
     2101 cggcgtcgag gtcgtaggcc aggctgtggg tgatcagcag gtagtacggc tcgtcgccgt
     2161 cgggctgccg gtgccgggcc gaccagggga ggacggcgag gtaggccagg aacggcagca
     2221 ggaagaagac ggcggccggc cgccgcggca ggcgctcgcc caggagcggc cgggccgcgg
     2281 cgagctgccg ggcgaggcgg accgccagca ccaggaggag gccggccgcc accgcctcgc
     2341 cggcgcgggc gaggcccaga ggggagcggg cgagggccag gagcaccaga ccgccgagcg
     2401 cggccagctc cccggcggtg ccggcggcgg cgaggggcct ccccccgagg ctctccacct
     2461 gccggcggac caggtggaag ccggcgagga gcagcggcag gatcgccagc gtgccccgga
     2521 atgggtcgtc cgccggcagc gacaggaacc gggagaggag ggcggcggcc acggccgagc
     2581 cggcgagcac cccggccgtc ctcagccgct ccccggagag ccagggcttc atggccggac
     2641 ctccacctgg tggacggcga ggctcacctc cggcggcagc cccggcgccc gctcgacgcg
     2701 caggcgcgtg aaccgcccgg gccggtcgag atcccaccgg ctccggaagc gctgggcgaa
     2761 gaagtccccc gccacccagg agacccaggc ctcgggcgac cgcaggcggg cggtccgctc
     2821 gacgtccggc cgccgcgccg cccactcgcc ggtcccctcc cccgcccgca gagtccagct
     2881 caccgaccgg cccgcgggat cgctcagcct gaagaccgcc accggcgtcc cgttctccag
     2941 gccggcgccg ttggcgagag cggattccac caccaggctc cctaccgggt gggccggcgc
     3001 cggcagggcc gcgtcccggg aggggtaggt ggcgtcgagc cgcaccgccg tctcgggagg
     3061 caggagcagg gttccgggcc gcggcagccg aatcaggagg acggcgaaga ccagcccggc
     3121 cagcaggaag gaaggaagga gacggacctc cggccggcgc tcgcgccaca ggccgaaggc
     3181 ggcgagggcg gcggcggcca aggccgggac ccaggagagg aacggctcgg tcgtcagtcc
     3241 caggagaccc aggacggcga ggacgggcgc ctctctcaac catggatagg acgccagcag
     3301 ggccgtcccc aggaggagca tgccggtcca taccttctga agaggagccg ccgggccgtc
     3361 ccggcgcagc agcagggccg ccaatgccag cggcgccgcc agcgcgggcc gctccggcag
     3421 gagccagggg ctcgcggcga ggaagaggac gacgaggagg cgccggacct gcctgcggtc
     3481 gatccacagc agcgcgggaa gcagcaggac caacaggaag gcgtcccggg gctcgcccag
     3541 ggccatgccg ggccggagga gccagaccgc cagcgcgccg agggggaggg ccagagccgg
     3601 caccggccaa gcgagggcca gcccggcggt ggccgccgcc aaccccggcc acggcttcca
     3661 ggcgatcggg gcgaggagga cgagagcggc cgccaccctg gcggtcttgc tcaggtcgcg
     3721 ccgggccagc aggtcgcccg cggcgagaga cgtcagcgcc agggtggtca gcacgttcca
     3781 acccggaccc acgaggaccg cgctccccgc ggcgagcagc gccgggtgcc cgccgtagtg
     3841 cctggcgagg accgcggtag caatcccccc ggcgaccggc agggcgagca gcgccaccag
     3901 tgtccgcgcc gacgcctcgg cgtcgaacag cagctccccc cgcagcagga actggacgct
     3961 cagggagagg gggaccgcca ccggcagagg gaggtccggg agcaccccgc ccagccgccg
     4021 tgccgcggcc acggccaggg tcgcggtgag gaccacggcc acggccgcgc cacggcccgg
     4081 cccgggcggc agcgcccaga cggccgccgc cgccagcacc gagccggcgg ccgcctccac
     4141 ccccggccgg ggcgccaggg cggtggccgc gagcagcacc gccgccgcca gcaccgccag
     4201 ataggcactc tcggcgccgc cggccgggtc cagctcgcga gctgcgaagc tcgccgtggc
     4261 cgcgaaggcg gccagcggca cgaggaagcg gagcagctcc tcgaggagcg gaccgcgtcc
     4321 ggcaggcgag ttgtcggcgc cgggggcgct aggggcgtcg aggtcggtcg tgatcacgag
     4381 cagcctgtta ctggcgggtg gcggggtctt cgcgtattca agccgaggcg gatgttaccc
     4441 gcttccggcc ggaacgcgcc gattctcccg cggatcgtct ctgtctatat gtacggatga
     4501 aaccccctcc gcgtcaccag gagggcgagg ggatgccgcg gcgcggcggt ggacaccctc
     4561 ccggggctcg ggctagactg cccgccatgc tctcccgaga cctcctgcgc gacgacttcg
     4621 agacggtccg ccagacgctg gcgacccgca acatggaccc ctccctcctg gagcgctggc
     4681 agcgcctgga cggcgagcgc cgtgcggcgc tggtcgaaac cgaggagatc aagcgccagc
     4741 gcaacgaggc gagccgcgcc atcggcaaga tcaagcagca ggggggcgac gccgccgggc
     4801 agatggccga ggtcggccgc atgaaggggc ggatcgagga gctggaggcg cgcctcgcca
     4861 ccgtcgagca ggaggtggag tccatcgaga ccgccctccc caacctgccc cacgagagcg
     4921 tcccggtcgg caaggacgaa tccgacaacc gcgtcgaacg ggtggtgggc gagccccggg
     4981 ccttcggctt cgagcccaag gcgcactggg acctggggcc ggagctcggc atcctcgact
     5041 tcgagcgcgc
//
LOCUS       DASPJI010000141         6486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_410500, whole genome shotgun sequence.
ACCESSION   DASPJI010000141 DASPJI010000000
VERSION     DASPJI010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410500"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="VEL74_03830"
     CDS             complement(<1..1180)
                     /locus_tag="VEL74_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYG61687.1"
                     /translation="MRWPCRLCWLWIALPLGLLGCGAETSAPWRQVDLWATPQSAGTP
                     SVRRTAPLGPEEVRNPALLQTAGPAGRRRVERLSAATVNALEQAAGATAAWRLELGAS
                     PYFAFTPLPAPGRSCPCAYRVAVRDGAGELSELRRIEMEPGAEPGTAAAPATVYVDLA
                     RYAGRQVELVLALEGPQGASALWGSPAVYDRGRPASPPRDRERPNVLILGLDTLRADA
                     LGAYGRVPSVTPAMDRLAGESDVWLEAFSCFNVTNPSFVSLMTGLYGKHHGIYDFDTR
                     LAPSQTTLAEVFSGLGYDTRAILSIDHLADARSGLGQGFGDVVVPRDQFAAEYAVGAA
                     MDWIGRRERPFFLWLHLFDAHTPHTPPQPFASGFRPETLAGLVPPRDWLAFRSPGPRG
                     Y"
     gene            complement(1288..5949)
                     /locus_tag="VEL74_03835"
     CDS             complement(1288..5949)
                     /locus_tag="VEL74_03835"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HYG61688.1"
                     /translation="MPSNRWGDLQPAAILGDRTAFPFHNEPERDHPFWFSVDIENNFM
                     FVATGRGLEVWDLRSNPAAPSLLGYGYAAANFSASGPIYNLFPIWQHTDKDWFLTDLD
                     APVGVDDILAIAGQDQGLSIWNTADKTRPTVFFQDRGTYTEATYTATLGGTQYAFALG
                     DRAAIAGVYLYDMSAARQYNRCGEDLEDFVNHCPGVYRGKLPGPSGGSGIDGVGNFLV
                     VGRASGFDIWNVANPMAPIKVMSSSSGARGVAIWESGNKIYLATVRAVGVAPNSEMRI
                     FDVSCIRSGLCSPTPLAGPISVPGGGTETTRHVTFSMGAGKPFLYLGGEEQAACAVQR
                     EYLFDVSNPANPVEITPKVHPEGYWGWYYELCPNEIPGNGPTGSPNPDGGFNWVAPLR
                     GKFNGDYFYRAAYSILDVHKLAAASPPVADFSWGPAQVYPGVPVAFTDLSTGSPNAWT
                     WTFEGGSPASAGAQNPSGIVFDSVGAKNVHLRVNNPAGVSQPKTKPVNVLSPQPAVAG
                     VTANLTAAKICQPVTFTASATGRPALSYQWTVLDAQGNPLAAPNSTGTGNPFTWNTQA
                     VPPGNYEVRVVVATPVANDGSATFDTYQVSPSVPAVQLAALPALPASFTATNDPFTSG
                     TVSFHVNVPGATEWRWDFDDDGDHNPETNPGTWTSDPIAGPNPTHTYASIGTRQVRVA
                     VRNCLQPVGLSSDPLVVNVTQVTPLKAQFSVSVFGCSSGVCFIDANAPVKFNDSSTGA
                     ELWDYDWDGNGTFEDAGNTKARDTHSYPSQGSYTPKLRVRRGTESDVFTLPETLVVGP
                     PVVPQTPSISVSGPTSGQPNQALSYTATASNCSPASTWSWSATGGTVAGSGSTVSITF
                     SSNGSKTVSVTNSGCAGATGSRSVTISTGGNPPPPPPPPPGLRAAYVFSPAAPVAGQA
                     IAFDATSSGGSPTGYVWDFGDGTAFGSGAQVQHTYAKEGSYKVQLSVTGPGSCPPAPF
                     CENATTKTVVVGNGVPPLVPAFQTNATCISEFGLEVCNAETGKAVQFTDASTGGNITT
                     RTWSFGDGQTGSGSSVSHAFAKEGTFTVTLTIGNGSSTASATRTFKVTGPDTPASQKK
                     VVVLPWIAQTRGALVQSSDLYIYNPASGPIEVSIQFLKRGTPEANPPKVTRTIQPGAT
                     LFTADVLSGLFNRENIAGFITVTAEEGAEPIITSFNTTFQDDGTQFGQTIPGVSLSQS
                     GAKATQPLQHLVGLNDNTDRLAYFGVSNPGDTQATYRLRFFDHLGTQIGQSADMPLSP
                     YGQQQFQIKQIRDQFGISDEDDYRVEVETKVGGQLFPYAANLRLASEDPSFVESVSPS
                     DAKAYLVGVFNTPGIGGSRWQSDVVLSNPSNQVVITEVRFLSVGVQSAPTQPVTITLQ
                     PGETQRIDNVLATKWGLNQTVGVLLFESNSPNGSFPVLLGEVYDNAKPEKRFGQTMTA
                     QVAADVAGNGKSQILVGLRQDSSYRSTVTLYNPSAANTTFDVVYRGLDGKVLGTLANQ
                     ALAPGKARQINPGQHPLPAQGVPDGFTVEIKVKGGSLLATGQVVNNRTNDPAFIRGRV
                     R"
BASE COUNT         1010 a         2143 c         2335 g          998 t
ORIGIN      
        1 ggtacccccg cggcccgggg ctgcggaacg ccagccagtc ccgcggcggc accagcccgg
       61 ccagggtctc cggacggaag ccagaggcga acggctgggg aggggtgtgg ggcgtatggg
      121 cgtcgaacag gtggagccag aggaagaacg ggcgctcgcg ccggccgatc cagtccatgg
      181 ccgcgccgac ggcgtactcg gcggcgaact ggtcccgcgg gacgacgacg tcaccgaacc
      241 cctgtcccag accggagcgg gcgtccgcca ggtggtcgat ggagaggatg gcgcgggtgt
      301 cgtaccccag gccggagaag acctcggcca gagtcgtctg actcggcgcc agccgcgtgt
      361 cgaagtcgta gatgccatgg tgcttgccgt acaggccggt catcagcgag acgaagctgg
      421 gattggtgac gttgaagcag gagaacgcct ccagccagac gtcgctctcc ccggccagcc
      481 ggtccatggc cggcgtgaca ctgggcaccc ggccgtacgc ccccagggca tcggcgcgga
      541 gcgtgtcgag cccgaggatc agcacgttgg gccgctcccg gtcccgcggg ggcgaggccg
      601 gccggccgcg gtcgtagacg gcggggctcc cccagagggc cgacgcgcct tgtggcccct
      661 ccagcgcgag aaccagctcg acctggcggc ccgcgtatct cgcgaggtcg acgtacaccg
      721 tcgccggcgc cgccgcggtc cccggctcgg cccccggctc catctcgatg cgccgcagct
      781 cggagagctc gccggctccg tcccggaccg cgacgcgata cgcgcaaggg caggaccgtc
      841 caggcgccgg aaggggggtg aacgcgaaat agggggaggc gccgagctcg agccgccacg
      901 cggccgtcgc gcccgccgcc tgctccagcg cgttcaccgt ggcggcggag aggcgctcga
      961 cgcgcctccg ccccgccggc cccgccgtct gcagcagggc cggattgcgg acctcctccg
     1021 gccccagcgg cgccgtgcgg cggacggacg gcgtcccggc agactgcgga gttgcccaga
     1081 gatcgacctg ccgccagggg gcggaggtct ccgcaccgca gccgagcagc ccgagcggca
     1141 gcgcgatcca gagccagcag aggcgacagg gccatctcat cccggtgggg aatcctagcg
     1201 gaaagacaaa ggccgcggcc ctcgaaagag ccgcggcccg gactggaaga aggccgccgg
     1261 ctcagccggc ggctcgaggc gactacctca gcggacgcgg ccccggatga acgccgggtc
     1321 gttggtccgg ttgttgacca cctggccggt cgccagcagg ctgccgccct tgaccttgat
     1381 ctcgacggtg aaaccgtcgg gaacaccctg ggccggcagc gggtgctggc cagggttgat
     1441 ctggcgggcc ttgccgggag ccagggcctg gttcgccagg gtgcccagca ccttgccgtc
     1501 gaggcctcgg tagaccacgt cgaacgtcgt gttggccgcc gaagggttgt agagagtgac
     1561 ggtgctgcgg tagctggagt cctgccgcag gccgacgagg atctgcgact tgccgttgcc
     1621 ggccacgtcc gccgccacct gcgccgtcat cgtctggccg aaccgcttct ccggcttggc
     1681 gttgtcatag acctcgccca gcagcaccgg gaaggagccg ttcgggctgt tgctctcgaa
     1741 gaggaggacg cccacggtct ggttgagccc ccacttcgtg gcgagcacgt tgtcgatccg
     1801 ctgcgtctcg ccgggctgca gggtgatggt caccggctgc gtcggagccg actgcacgcc
     1861 gacgctcagg aagcgcacct cggtgatcac gacctggttg ctggggttgg acagcaccac
     1921 gtcgctctgc caacggctgc cgccgatgcc cggagtgttg aagaccccca ccaggtacgc
     1981 cttggcgtcc gacgggctca ccgactcgac gaaggacggg tcctccgaag cgaggcggag
     2041 gttggcggcg taggggaaga gctgaccgcc caccttggtc tcgacctcga cccggtagtc
     2101 gtcctcgtcg gagatcccga actgatcccg gatctgcttg atctggaact gctgctggcc
     2161 gtacggcgac agcggcatgt ccgccgactg gccgatctgc gtgcccaggt gatcgaagaa
     2221 ccgcagacgg taggtcgcct gggtgtcacc cgggttgctg acgccgaagt aggcgaggcg
     2281 gtcggtgttg tcgttcaggc ctaccaggtg ctggaggggc tgggtcgcct tggcgccgga
     2341 ctggctcagc gacacacccg ggatcgtctg gccgaactgc gtgccgtcgt cctggaaggt
     2401 ggtgttgaac gaagtgatga tgggctcggc gccctcctcc gcggtgaccg tgatgaagcc
     2461 ggcgatgttc tcgcggttga acaggccgct cagaacgtcg gcggtgaaca gcgtcgcccc
     2521 cggctggatg gtgcgggtca ccttcggcgg gttggcctcc ggcgtgcccc gcttcaggaa
     2581 ctggatggac acctcgatcg gacccgaggc cgggttgtag atgtagaggt cgctcgactg
     2641 caccagcgcg ccccgggtct gggcgatcca ggggagcacc accaccttct tctgcgaggc
     2701 cggcgtatcg ggacccgtga ccttgaaggt ccgcgtcgcg gacgcggtcg aggagccgtt
     2761 gccgatggtg agggtcacgg tgaaggtgcc ctccttggcg aacgcgtgac tgacggagct
     2821 cccgctgccg gtctggccgt cgccgaagct ccacgtccgg gtggtgatgt tgccgccggt
     2881 gctggcatcg gtgaactgca ccgccttgcc ggtctcggcg ttgcagacct ccaggccgaa
     2941 ctcggagatg caggtggcgt tggtctggaa ggcgggcacc agaggcggca cgccgttgcc
     3001 cacgacgacc gtcttggtgg tggcgttctc gcagaacggc gccggcgggc aggagcccgg
     3061 gccggtgacg ctcaactgaa ccttgtagga gccctccttg gcgtaggtgt gctgcacctg
     3121 ggcgccggag ccgaaggccg tgccgtcgcc gaagtcccag acgtagccgg tcgggctacc
     3181 cccggacgag gtcgcgtcga aggcgatggc ctggcccgcc accggcgccg ccggcgagaa
     3241 gacgtaggcc gcccgcagac ccggaggcgg cggaggcgga ggcggcgggt tgccgccggt
     3301 ggagatcgtc accgaacgag agccggtggc gccggcgcag ccgctgttgg tcaccgacac
     3361 cgtcttcgag ccgttgctgc tgaaggtgat gctgaccgtg ctgccgctgc ccgcgacggt
     3421 gccgccggtc gccgaccagc tccaggtgga agccggcgag cagttggagg ccgtggcggt
     3481 gtacgacagg gcctggttcg gctgcccgga ggtcgggccg ctgaccgaga tgctgggggt
     3541 ctgcggcacc accggcggcc ctacgaccag ggtctccggc agcgtgaaga cgtccgactc
     3601 ggtgccgcgg cgcacccgca gcttcggcgt gtagctgccc tgcgagggat acgagtgggt
     3661 atcccgcgcc ttggtgttgc ccgcgtcctc gaaggtgcca ttgccgtccc agtcgtagtc
     3721 ccagagctcg gcgccggtgg acgagtcatt gaacttgacg ggcgcgttgg cgtcgatgaa
     3781 gcagacgccg ctgctgcagc cgaacacgct gaccgagaac tgggccttga gcggcgtgac
     3841 ctgggtgacg ttcaccacca gcggatcgct ggagaggccc accggctgca ggcagttgcg
     3901 caccgccaca cgcacctggc gggtgccgat ggacgcatag gtgtgggtcg ggttcgggcc
     3961 cgcgatcgga tcgctggtcc aggtccccgg gttggtctcc ggattgtggt cgccgtcgtc
     4021 gtcgaagtcc cagcgccact cggtggctcc cggaacgttg acgtggaacg acaccgtgcc
     4081 cgaggtgaac ggatcgttcg tcgccgtgaa cgaagccggc agggcgggca gggcggcgag
     4141 ctgcaccgcg ggcaccgagg ggctcacctg ataggtgtcg aacgtggcgc tgccgtcgtt
     4201 ggccaccggc gtggccacca cgacgcggac ctcgtagttg cccggcggca ccgcctgggt
     4261 gttccaggtg aacggattgc cggtgccggt gctgttcggc gccgccagcg ggttgccttg
     4321 ggcgtcgagc accgtccact gataggacag ggccggacgg ccggtggcgc tggcggtgaa
     4381 ggtgacgggc tggcagatct tcgccgcggt caggttcgcc gtgacgccgg cgaccgccgg
     4441 ctgcgggctc aggacgttca ccggtttggt cttcggctgg ctgacgcccg cggggttgtt
     4501 gaccctcagg tggacgttct tggcgcccac gctgtcgaag acgatcccgc tgggattctg
     4561 cgcgccggcg ctggccgggc tgccgccctc gaaggtccag gtccaggcgt tcgggctgcc
     4621 ggtcgacaga tccgtgaacg ccaccggcac gccggggtag acctgagcgg gaccccagga
     4681 gaagtcggcg accggcggcg acgccgccgc cagcttgtgg acatcgagga tgctgtaggc
     4741 cgcgcgatag aagtagtcgc cgttgaactt gcccctgagc ggggccaccc agttgaagcc
     4801 accgtcggga ttggggctgc cggtcggacc attgcctgga atctcattgg gacacagctc
     4861 gtagtaccag ccccagtagc cttcggggtg gaccttcggg gtgatctcca ccggattggc
     4921 cggattgctg acgtcgaaca ggtactcgcg ctgcaccgcg caagcggcct gctcctcgcc
     4981 gccgagatag aggaagggct tgcccgcccc catggagaac gtaacgtggc gcgtcgtctc
     5041 ggtgccgcca ccgggaacgc tgatcggacc ggccagaggc gtcggcgagc agaggcccga
     5101 gcggatgcag ctcacgtcga agatccgcat ctcggagttg ggcgcgacgc cgactgcccg
     5161 caccgttgcc agatagatct tgttgcccga ttcccatatg gcaacaccgc gcgcgccgct
     5221 ggaggaggac atcaccttga tcggagccat cggattggcc acgttccaga tgtcgaagcc
     5281 gctggcccgc cccaccacga ggaagttccc aacgccgtcg atcccgctgc cgcccgaagg
     5341 tccgggaagc ttgcctcggt agactccggg acagtgattg acgaagtctt ccaggtcctc
     5401 cccgcaccgg ttgtactggc gggcagcgct catgtcgtag aggtagaccc ccgcgatcgc
     5461 cgccctgtcc cccagggcga aggcgtactg ggtaccgccc agcgtcgcgg tataggttgc
     5521 ctctgtataa gtcccgcggt cctggaagaa gacggtggga cgggtcttgt ccgcggtgtt
     5581 ccagatcgaa agcccctggt cctggcccgc gatcgccaga atgtcgtcca ctcccacggg
     5641 agcatccagg tccgtcagga accagtcttt atccgtgtgc tgccagatcg ggaagaggtt
     5701 ataaatcgga cccgacgcac taaagttcgc agccgcgtag ccgtagccca ggaggctcgg
     5761 cgcggcggga ttgctccgca ggtcccagac ctccagccct ctcccggtag ccacgaacat
     5821 gaagttgttc tcgatgtcga cggagaacca gaagggatga tccctttccg gctcgttatg
     5881 aaaggggaag gcggtgcggt cacccagaat ggcggcgggc tgcagatccc cccaccggtt
     5941 gctcggcacg ggagccgcgg gagcattgct cctgtcgaag gcctctgctg ccggtactgc
     6001 cagcgcgagc gtcaggcacg ccacaagcga aacgctccgc cacgaccgac cctggagaga
     6061 acggatcgtt gagttgagat tcatctcttc tccacctcca tcagagaggc cactgagacc
     6121 ccgattagtc gacgcagcct gcgcaacccg cgctcccccc gtgctggaca ggaccgagtg
     6181 ccggctggct cctccgcgct gctcatcagc caggagacac cgcgcaagag ccgaagccga
     6241 aggaagccgg gcgggtcgcc gaacgtcttt acaacagata tctatgggat cttagcatac
     6301 ccggggaatc ctggcggaaa gacaagggcc gcggccctcg aaagagccgc ggcccggact
     6361 ggaagaaggc cgccggctca gccggcggcc tcgaggcgac tacctcagcg gacgcggccc
     6421 cggatgaacg ccgggtcgtt ggtccggttg ttgaccacct ggccggtcgc cagcaggctg
     6481 ccgccc
//
LOCUS       DASPJI010000142         5288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_122378, whole genome shotgun sequence.
ACCESSION   DASPJI010000142 DASPJI010000000
VERSION     DASPJI010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122378"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VEL74_03840"
     CDS             complement(<1..257)
                     /locus_tag="VEL74_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HYG61689.1"
                     /translation="MTTPTAEAPTAPPVPIMDLGRTRQRIEGELQRRWQRILDTSAFV
                     LGPELKEFERAWAEYLGVAASVGLANGTDALVLAAKALGLAP"
     gene            313..1821
                     /locus_tag="VEL74_03845"
     CDS             313..1821
                     /locus_tag="VEL74_03845"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HYG61690.1"
                     /translation="MLPRLRFYWVVVTCGAVIMALEIVSSRILAPYFGNSVYIWGSII
                     SVFLAALSLGYLWGGRLADRHPTMAALGRMITFAAFGQALLLMAGVPVTQWLAARTGG
                     ASAGPLLAATVLFGPTSVLLATVSPWAVRLAARDLGHLGNTAGRLYALSTLGSLAGTL
                     ACTFLLIPFLELREILGLLTALTAVTALVAFGERLRAEAPTAALAALLVVFAVARGAS
                     VARPAPGLLHERTTPYQTLRVYERDGVRRLESDRMQQSAVEMATGEPAVQYIRHVPFV
                     LLLRPDMDRMLALGMGAGSAAGYLQKRLPGLEMDNVDIDPAVPELARRFLLFRDSPRN
                     RVHVADGRSFVHASDERWDFIFSDTYIGGSVPFHMTTVQFLEEVKRRLEPGGVFGVNL
                     AAGLADPFSQAIYHTVEQRFRTTYVIQVKARANVLVLATDADPLPSDRLLARARELDR
                     RWRFEPTLESLAGSRAPVDLDASGVPVLTDEFAPVDRLIRLGQAARDWDRQQ"
     gene            complement(1775..3454)
                     /locus_tag="VEL74_03850"
     CDS             complement(1775..3454)
                     /locus_tag="VEL74_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61691.1"
                     /translation="MPVPDSPDSRSRAPVWLLLAGALACLGYFAVEAWLLSFDLGFPL
                     DDTWIHLQFARNLAAGQGLSYNPGELVTGSTAPLWTALLALLIPLPGNVVVWTKLLGV
                     VLHIAGIDAVWRLARELGLSRGLAALAAGLTLATSWLVWSALSGMEIPLFVLLSVWGM
                     TLHLRERAASLEGPPRPPLSFALFAAAALARPEGLLLLVLAAVDRCLVFEREGEAGEE
                     RLRWRRPDARAVLIGLALAACALVGPLLFYRWAGGSFLPTTFAAKNGDLRRWLPDLQY
                     IYMVAGILFRPQPWMTLTAGAGVLALVERLGGPPGPRGDRGLLPALWLIGLPLAYSIM
                     SPTGKGMIAGNFGRYYFPLFPVLVVLGVLGLERAARVLGPRVRAGRLGLPLGALLVAL
                     ILWPTIGSLVQGAGRYAQNVANVQDSDVAIARWLAPRLAPEAVLAVNDIGAIKFLLPN
                     QIVDLASIATPEIRREVGRDTAAGMSWSDAMTAAIARRQPDYIVVFPSWVPFLARDPR
                     FPPVRSLAIPNNITMGGDEIVVYATPWTRYPLREAAPTAAGPSPAPPGPGG"
     gene            3504..4940
                     /locus_tag="VEL74_03855"
     CDS             3504..4940
                     /locus_tag="VEL74_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYG61692.1"
                     /translation="MPQPRTWMDRRRRQAARIALYGSLVLVGGVTAGLVKLLDRPLYG
                     TANEEWIERDYLSMPEVKLLQEYVRIDTSVTTGDEAAGARFLAAQLEAAGIPARVELL
                     GERRANVYALLEGDDPRPLVLHNHIDVQPADPSQWFHPPFEAKIDLPWIYGRGVFDMK
                     SVAIAQLMAITDLKKSGVRPKRSVLFLATSGEERGSDFGVLWILREHPELVRGFWAVL
                     TEGGVLEARARDDFKFWGVEYAQKRFFDLLVCSDDRQRLEQVRNDLIQLGHTETDLRL
                     VPEARTLFKTYAMTRDSPELRRLLEEPESVLGDIATFRELPGYIRSMFRNEAVPFPVQ
                     EAPGGGYQMVIKLHLLPHQELTPEIIERLVPSWVTGGLPRVLRPAPVAKGGSPTDHPA
                     FKLVVRTLQEHYPKVPVGPYFLPWTATDSRFFRSAGVPSYGFSPFLIMNTDTLQVDQA
                     NERMALPGYVDGVALYREVVRRLAADTE"
     gene            5103..>5288
                     /locus_tag="VEL74_03860"
     CDS             5103..>5288
                     /locus_tag="VEL74_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYG61693.1"
                     /translation="MSRNRQKGFTLIELLIVVAIIGIIAALLIPNFLDALQKAKQKRT
                     VADIRNTGTAMFSWLTDQ"
BASE COUNT          798 a         1942 c         1824 g          724 t
ORIGIN      
        1 ggggcgaggc ccagcgcctt ggccgcgagg accagggcgt ccgtgccgtt ggccaggccg
       61 acgctggccg ccacccccag gtactcggcc caggcgcgct cgaactcctt gagctcgggg
      121 ccgagcacga aggcggaggt gtcgaggatg cgctgccagc gccgctggag ctcaccctcg
      181 atgcgctgcc gggtgcggcc gaggtccatg atcgggacgg gcggggcggt gggggcttcg
      241 gcggtcggcg tggtcatagg tctcggctcc tccggcgcgg gattgtatcc gcatctggca
      301 tactctcccg ccatgctccc ccgtctgcgg ttctactggg tggtcgtgac ctgcggcgcc
      361 gtcatcatgg cgctggagat cgtgtcgagc cgtatcctcg ccccctactt cggcaacagc
      421 gtctacatct ggggctcgat catcagcgtc ttcctggccg ccctctccct cggctatctc
      481 tggggcggac gcctggcgga ccgccacccc accatggccg cgctggggcg gatgatcacc
      541 ttcgccgcct tcggccaggc gctgctgctg atggccggcg taccggtgac gcagtggctg
      601 gcggcgcgca ccggcggcgc gtcggccggg ccgctcctcg ccgccaccgt gctgttcggc
      661 cccaccagcg tgctgctggc cacggtctcc ccctgggcgg tgcgcctcgc cgcccgcgac
      721 ctcggccatc tcggcaacac cgccggccgg ctctacgccc tgtcgaccct gggcagcctg
      781 gccggcaccc tggcctgcac cttcctcctc atccccttcc tcgagctgcg ggagatcctg
      841 gggctgctga cggcgctgac cgcagtcacc gcgctggtgg ccttcggcga gcggctgcgg
      901 gccgaggcgc cgacggcggc cctggcggcg ctcctggtgg tcttcgccgt cgcccgcggc
      961 gcgtccgtgg cgcggccggc gccgggcctc ctccatgagc gcaccacgcc gtaccagacg
     1021 ctgcgggtgt acgagaggga cggcgtgcgc cggctggaga gcgaccggat gcagcagtcg
     1081 gcggtggaga tggccaccgg cgagccggcc gtccagtaca tccggcacgt gccattcgtc
     1141 ctgctgctgc gcccggacat ggaccgcatg ctggcgctcg gcatgggggc cggcagcgcc
     1201 gccggctatc tgcagaagcg gctgccgggg ctcgagatgg acaacgtgga catcgatccg
     1261 gcggtccccg agctggcccg ccgcttcctc ctcttccggg acagcccacg caaccgcgtc
     1321 cacgtggccg acgggaggag cttcgtccac gccagcgacg agcgctggga cttcatcttc
     1381 agcgacacct acatcggcgg gtcggtgccg ttccacatga cgacagtgca gttcctggag
     1441 gaggtgaagc gccggctgga gcccggcggg gtgttcggcg tcaacctggc ggccggcctg
     1501 gcggacccct tctcgcaggc gatctaccac acggtggagc agcgcttccg gaccacctac
     1561 gtcatccagg tcaaggcacg cgccaacgtc ctggtgctgg ccaccgacgc ggacccgctg
     1621 cccagcgacc ggctcctcgc ccgcgcccgc gagctcgacc ggcgctggcg cttcgagccg
     1681 accctggagt cgctcgccgg ctcccgggcg ccggtggacc tggacgcctc cggcgtcccc
     1741 gtcctcaccg acgagttcgc ccccgtcgac cgcctcatcc gcctgggcca ggcggcgcgg
     1801 gactgggacc ggcagcagta ggcgccgctt cccgcaacgg atagcgggtc cagggggtgg
     1861 cgtagaccac gatctcgtcg ccgcccatgg tgatgttgtt ggggatcgcc aggctgcgga
     1921 ccggcgggaa gcgggggtcg cgggcgagga acggcaccca ggaggggaag accacgatgt
     1981 aatcgggctg gcggcgggcg atggcggcgg tcatcgcgtc gctccagctc atcccggccg
     2041 ccgtgtcccg tcccacctcg cgccggatct ccggcgtggc gatcgacgcc aggtcgacga
     2101 tctggttggg cagcaggaac ttgatggcac cgatgtcgtt gaccgccagc accgcctcgg
     2161 gcgccaggcg cggcgccagc cagcgggcga tggccacgtc gctgtcctgg acgttggcga
     2221 cgttctgcgc gtagcggccc gccccctgga ccagcgagcc gatggtcggc cagaggatca
     2281 gcgccaccag cagggcgccc aggggcaggc cgagcctccc cgcccgcacc cgcggcccca
     2341 gcacccgcgc cgcccgttcc agccccagca ccccgagcac gaccagcacc gggaacaggg
     2401 ggaagtagta gcggccgaag ttgccggcga tcatcccctt gccggtaggg ctcatgatcg
     2461 agtaggcgag ggggagcccg atgagccaga gcgccggcag caggccccgg tctcccctgg
     2521 ggccgggcgg gcctcccagc cgctccacca gcgccaggac ccccgcgccg gcggtcagcg
     2581 tcatccacgg ctggggccgg aagaggatgc cggccaccat atagatgtac tggaggtcgg
     2641 ggagccaccg gcgcaggtcg ccgttcttgg ccgcgaaggt ggtgggcagg aacgatccgc
     2701 ccgcccagcg gtagaagagg aggggcccga ccagcgcaca ggccgccagc gccaggccga
     2761 tgaggaccgc ccgcgcgtcc ggccgccgcc agcgcagccg ctcttccccc gcttcaccct
     2821 cccgttcgaa caccaggcag cggtccaccg ccgccagcac cagcagcagc aatccctccg
     2881 gccgggcgag cgccgcggcg gcgaacagcg cgaaggagag aggcggccgg gggggcccct
     2941 ccagagaggc ggcgcgctcc cgcaggtgca gggtcatccc ccagacggag agcaggacga
     3001 agagcgggat ctccatcccc gacagcgccg accagaccag ccagctcgtg gcgagcgtca
     3061 ggcccgccgc cagggcggcg aggccgcggc tcagccccag ctcgcgggcg agccgccaga
     3121 ccgcgtcgat gccggcgata tggaggacaa cgccgagcag cttcgtccac accacaacgt
     3181 tgccgggcag cgggatgagc agcgccagca gggccgtcca cagcggtgcg gtggagccgg
     3241 tgaccagctc ccccgggttg taggagagcc cctgccccgc cgccaggttg cgcgcgaact
     3301 gcaggtggat ccaggtgtcg tcgagcggga agccgaggtc gaacgacagc agccaggcct
     3361 ccacggcgaa gtagccgagg caggccaggg ctcccgccag gagcagccag acgggggccc
     3421 gggagcggga atcgggggag tccggaacgg gcatcgaggc gcaagcatac acaagaggcc
     3481 tgcgcccgtt tatcatgccc cgcatgcctc agccccggac ctggatggac cggcgccgcc
     3541 gccaggcggc ccgcatcgcg ctctacggca gcctcgtgct ggtgggcggc gtcaccgccg
     3601 gcctggtcaa gctcctcgac cgccccctgt acggcaccgc caacgaggag tggatcgaac
     3661 gcgactacct ctccatgccc gaggtcaagc tcctccagga gtacgtgcgc atcgacacca
     3721 gcgtcaccac cggcgacgag gcggccggcg cccgcttcct ggcggcgcag ctcgaggcgg
     3781 ccggcatccc ggcgcgcgtc gagctgctgg gggagcgccg cgccaacgtc tacgccctgc
     3841 tcgagggaga cgacccccgg ccgctggtgc tccacaacca catcgacgtc cagccggccg
     3901 acccgagcca gtggttccac ccccctttcg aggcgaagat tgacctgccc tggatctacg
     3961 gccgcggcgt cttcgacatg aagagcgtcg ccatcgccca gctcatggcg atcaccgatc
     4021 tcaagaagag cggcgtccgc ccgaagcgct cggtgctgtt cctcgccacc agcggcgagg
     4081 agcgggggag cgacttcggg gtgctctgga tcttgcgcga gcaccccgag ctggtccgcg
     4141 gcttctgggc ggtgctcacc gagggcgggg tgctggaggc gcgcgcccgc gacgacttca
     4201 agttctgggg cgtggagtac gcgcagaagc gcttcttcga cctcctcgtc tgcagcgacg
     4261 atcgccagcg cctcgagcag gtccgcaacg acctgatcca gttgggccac accgagaccg
     4321 acctgcgcct ggtccccgag gcccgcaccc tcttcaagac ctacgcgatg acccgcgaca
     4381 gcccggagct gcgccgcctg ctcgaagagc cggagtcggt gctcggcgac atcgccacct
     4441 tccgcgagct gccgggctac atccggtcga tgttccgcaa cgaggcagtg cccttcccgg
     4501 tgcaggaggc gcccggcggc ggctatcaga tggtcatcaa gctccacctg ctgccgcacc
     4561 aggaactgac ccccgagatc atcgagcggc tcgtgccgtc gtgggtcacc ggcggcctgc
     4621 cgcgggtgct caggccggcg ccggtggcca agggggggag ccccaccgac cacccggcct
     4681 tcaagctggt cgtccgcacg ctccaggagc actatccgaa ggtgcccgtg ggtccctact
     4741 tcctcccctg gaccgccacc gactcccgtt tcttccgatc cgccggcgtg ccgtcctacg
     4801 gcttctcccc cttcctcatc atgaacaccg acaccctcca ggtggaccag gccaacgagc
     4861 ggatggccct gcccggctac gtcgacgggg tggctctcta ccgcgaagtg gtacgccggc
     4921 tggcggctga cactgagtga caggcccgca aggccggggc tgtcaaaaat tgtccacccc
     4981 gaaatgcaaa ccactggaaa caaaggatct tcaacttggc atcagccctg ctttatagac
     5041 tgagcgagac gccccggact ccaccgcggg caggatcatg aactagggga ggagacccaa
     5101 tgttgagcag aaaccgtcag aagggcttca cgctgatcga gctgctgatc gtcgtcgcca
     5161 tcatcggcat catcgcggcg ctgctgatcc cgaacttcct cgacgccctc cagaaggcga
     5221 agcagaagag gaccgtggcc gacatccgca acaccggcac ggcgatgttc tcgtggctga
     5281 ccgaccag
//
LOCUS       DASPJI010000143         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_694225, whole genome shotgun sequence.
ACCESSION   DASPJI010000143 DASPJI010000000
VERSION     DASPJI010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694225"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VEL74_03865"
     CDS             complement(<1..273)
                     /locus_tag="VEL74_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61694.1"
                     /translation="MPKPVPLVRIAEALEMPSEGSQVYFDRETGKVHFLPDEYVSAAN
                     DEDTDLDRYGAWQRELVELARTILRDTQGRYVELPDPSEIDEHRILA"
     gene            complement(439..798)
                     /locus_tag="VEL74_03870"
     CDS             complement(439..798)
                     /locus_tag="VEL74_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61695.1"
                     /translation="MPNRKSILALFLFGALLVPGVRSASAADGISGECRQNETRAADW
                     LGGESDPKLPSGLTPAPDFKVTYTCGTCGEASCSGKTVQSSCTSSSGLPGVCLALFSG
                     SQPARCPYGGYQCACLD"
     gene            complement(977..1276)
                     /locus_tag="VEL74_03875"
     CDS             complement(977..1276)
                     /locus_tag="VEL74_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61696.1"
                     /translation="MNSSRSRALRLLAPLALAAVAVWSGATKEASAQTTCPLHLIKVT
                     YYSEPEKINAVGTCTSYCSYTTPSNCTGTQTQYSTVNYRIACPFCIGSPYPTFGN"
     gene            2176..>3083
                     /locus_tag="VEL74_03880"
     CDS             2176..>3083
                     /locus_tag="VEL74_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYG61697.1"
                     /translation="MPEGGSLVEVTCRTVQSRLLLAPRAYVRETVLGVLARAKRLYPV
                     KLICFVFTSNHYHLLLEVEDAAEMAAFMGYLNSNLARGLGRLADWKEKFWGRRYQHIV
                     VSAEEEAQIARLKYALSHGAKEGLVGSPREWPGAHAVNALLSGDPVEGVWHDRTQEYR
                     YRLRGKEPAPGQFATLETLTLDPLPCWKHLPPEEYRQRIAELVAEIEEDARVAREAKG
                     VEPLGADAVRRQPPHTRPNRTKRSPAPLFHAASKKAYLNLRDAYSRFVAAFRDAAEKL
                     KAGDRTVAFPEGSFPPAAPFVRPAFT"
BASE COUNT          491 a         1005 c         1072 g          515 t
ORIGIN      
        1 ggcgaggatg cggtgctcgt cgatctcgga cgggtcgggc agctccacat agcggccctg
       61 agtatcccgg aggatggtcc gggcgagctc gaccagctcg cgctgccagg cgccgtacct
      121 gtccagatcg gtgtcttcgt cgttcgcggc ggagacgtat tcgtcgggca ggaagtggac
      181 cttccccgtc tcgcggtcga aatagacctg cgacccctcg ctgggcatct cgagggcttc
      241 ggcaatccgg acgaggggga cgggtttcgg cataggggcc gatcccatgc aaagcaagga
      301 ccgcgagggt cggcaaagag ggccgcccgt ccggaggcgg cgggcggacc ctcggacatc
      361 agacagctct ggccgccgcg gtccgccggc gcgaggagca ggccaaccgt gcctgccccc
      421 tgccggtgtt ctcccgtctc agtccaggca cgcgcactga tagccgccgt acgggcacct
      481 cgccggctgg ctgccggaga agagcgccag gcagactcca ggcagaccgg aggagctggt
      541 gcaagagctc tggacggtct tgccggagca cgacgcttcg ccgcaggtgc cacaggtata
      601 tgtgactttg aagtccggcg cgggagtgag gcccgagggg agcttcgggt cgctctcccc
      661 gccgagccag tcggcggccc gggtctcgtt ctgccggcat tcgccggaga tgccgtcagc
      721 ggcggaggcg ctccggacgc ccgggaccag cagggctccg aacaggaaca gggccaggat
      781 gctcttgcgg ttcggcatga cactccctcc tcttggttgt cacggcgcgg ctccacgccc
      841 tgcgccgtac ctacagggag aggtgctccc cggaggctcc gccgagtatc agcggcggcg
      901 ccggaggggg agcggaaggg cgtgaccggc ccccggaacg gcagagcagg tcacggtatc
      961 cgttggctag attcctctag ttcccgaagg tggggtaggg agacccgata cagaaggggc
     1021 acgcgatcct gtaattcacc gtgctgtatt gggtctgggt gccggtgcag ttggatggcg
     1081 tggtgtagga gcaatacgag gtgcaggtgc cgacggcgtt gatcttctcg ggctcggagt
     1141 agtaggtcac ctttatcagg tgcagggggc aggtggtctg ggcgctcgct tccttggtcg
     1201 cgcctgacca gacagcgacg gctgccagtg ccagcggagc caagagacga agggctcggg
     1261 agcggctcga gttcatggtg cctccttgcg cgtacgcgcg aaattcgtat ggggatcgta
     1321 agcggtgtcg gagcccacga caagcgccgc cgggtccccc ctctccccgg acaggcctga
     1381 atctggacca cgatccctcg cggccgcatc gtcttccccg cattttggat cactctcccc
     1441 gggggaggga tcactcctgg cccgccggga tcactctccc cgcgtccgga gcgttcccgc
     1501 ggcccggttc gtgttccggg gcctggggag gatcactctc cccgcttccc gcacccttcc
     1561 cccggccgga aaatgatctc cccgccttcc cgaatgctcg ccccgccggg ttttgtgatc
     1621 ccccgcgcgt cccgaagggc cgtctgaggg gggagagcct tcggcgtggg atcccgagac
     1681 acggcggccc accggcgggc cgctcctcct ggccgtatcg ccgtccaacc acgtcccgcg
     1741 agcgcggcgc gccccgcctc gacgccgcgc tcgggagaca tgtccgtcaa ctgtcaaaga
     1801 accgacaggc tgctcctcct ggattgagtc aaggagtggc ggcacgcgta aattctcggc
     1861 tcggttgact tgggtgtcaa tacctggcgt agaatggagt cccgatggga caggtagatc
     1921 ccgtcggtgg ggttcattga caactgaatc gtttgggggt gccgccgggc tcaaggccgc
     1981 gccccccgga tctcccgcgc cctgaacgtg gaggcggagg agcaggcctc cgcctccgtg
     2041 tcccgccccg aaggggccca gggatagacg ttctctcctc ccggttcaga catagatgct
     2101 cgcggatgcg ctaaaattga gcgatttctc cggcgttttc cgtcttttcc tggcatgccg
     2161 agaaaactcc gtcatgtgcc agaaggcggt tccctcgtcg aggtgacctg ccgcaccgtc
     2221 caaagccgct tgctgctcgc ccctcgtgcc tacgtccggg agaccgtcct cggtgtcctg
     2281 gcgcgggcga aacggctgta ccccgtgaag ttgatctgct ttgtctttac ctccaaccac
     2341 tatcacctct tgctggaagt ggaggacgcg gccgagatgg ccgcgttcat gggttacctg
     2401 aactccaacc tggcgcgggg gctcggccgc ctggccgact ggaaggagaa gttctggggc
     2461 cggcgctacc agcacatcgt ggtcagcgcc gaggaggaag cgcagatcgc tcgcctgaag
     2521 tatgccctct cgcacggggc caaggaggga ttggtgggca gcccccggga atggcccggt
     2581 gctcacgcgg tcaacgccct gttgagcggc gatcccgtgg agggggtctg gcacgaccgt
     2641 acccaagagt accggtaccg tctgcggggc aaggaacccg cgccgggcca gttcgcgacc
     2701 ctcgagacgc tgactctgga tccgctgccg tgctggaagc acctgccgcc agaggagtac
     2761 cgacaaagga tcgccgagct ggtggccgag atagaagagg acgcccgggt ggcccgggag
     2821 gcgaagggtg tcgagcctct gggagcggac gcggtgcgga ggcagccgcc gcacacccgt
     2881 ccgaaccgga cgaagagatc acccgcgccg ctgttccacg cagcgagcaa gaaggcctac
     2941 ctgaatctgc gggacgcgta cagccggttc gtggctgcct tccgggacgc cgccgagaaa
     3001 ctgaaggccg gcgaccggac ggtggcgttc ccggagggga gcttcccccc ggcagcgccc
     3061 ttcgtgcggc cggccttcac gta
//
LOCUS       DASPJI010000144         3560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_636887, whole genome shotgun sequence.
ACCESSION   DASPJI010000144 DASPJI010000000
VERSION     DASPJI010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636887"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VEL74_03885"
     CDS             <1..296
                     /locus_tag="VEL74_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HYG61698.1"
                     /translation="ADLNAAQCGYCTPGILMTAKALLADNPAPTADEIREALSGNLCR
                     CTGYHKIVEAVEWAAARMRGEDGPPPPRVLYGDPLPEPAGSPRLRVLPSLSAD"
     gene            323..2911
                     /locus_tag="VEL74_03890"
     CDS             323..2911
                     /locus_tag="VEL74_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYG61699.1"
                     /translation="MAEPVQKPTPDSEPRPTREPNIIGKPFRRVDGRAKVTGATRYAD
                     DLAFPRMVYLRLVRATIPHALVTRIDFSEAEKVPGVLGFLTGRDFPVPFGILPVSQDE
                     HALCPDKVRFVGDPVAAVAAVTEDAAYEAALKVRVEYEPLPTISSVEEALETPEPRIH
                     DYGDHGNVHKAVSMTFGDVDGGFAAAGEVFEDVFFYEGNTHLPMEQHAAVAVPEDDDR
                     VTLYSSTQTPHYVHRAVSKVLGLPASRVRVIACSNGGGFGGKSDPFNHEIVAAKMALK
                     LGRPVKVTLTREEVFYCHRGRHPVLMQVRTGIRKHDDGRVEITAQHLKTALDGGAYGS
                     YGVASTYYTGALQTVTYKLPAYRFEGVRCFTNKPPCGPKRGHGTPQPRFGFEVHLDKI
                     AVATGTNPADLRLGMLADPGTVTANWLRIGSMGLRRCIEAVVEGSGFRERWGKLPEGR
                     GLGLACGSYLCGAGLPIYWNHMPQSGVQIKLDRSGGVAVFCGESEIGQGSDSVLAAVV
                     AEVLGVSLSDIRLCVADTDLTPVDLGSYSSRVTLMMGNAAREAAERAREMIAKAVSDQ
                     LEVPGARLVFAENRVFDAENPEAGMTFQEAVIATEARFGTLGTTGGYIPPRSPGRYRG
                     AGVGPSPAYSYSAAVVEVEVDPATGLWSPVHVWMAHDIGRSLNPVLVVGQVEGSVYMG
                     LGEVMMEEQSFRRLPRHLSRALVHEFPSMLEYKSPTFHEMPPVTTYLIEEPDPEGPFG
                     AKEVGQGPLLPMMPAVANAIHDAVGVRVDQVPIHPHMIVKALAAKAKGGEPRFGPRAF
                     PDVDFGENLVVPTPEQGGDGTAINDYRENLRSGMRSASGTMMSREEALKRKKVAALTT
                     DLKGAK"
     gene            3006..>3560
                     /locus_tag="VEL74_03895"
     CDS             3006..>3560
                     /locus_tag="VEL74_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HYG61700.1"
                     /translation="MLRLPPFQLVQPRTLPEAAAALSGEGAANGELVRLVAGGTDLWP
                     NMKRRHQKARAVVSLMGIPGLRGVDANGEVRIGATTILEDLVRHEAVRSRYPAFARAV
                     ASISSPPLREMGTIGGNVCVDTRCTYYNQTEEWRRSIGYCMKAEGEICWVATSSPRCL
                     AHTASDSAPMLCALGARVRLTSQEG"
BASE COUNT          530 a         1323 c         1215 g          492 t
ORIGIN      
        1 tcgccgactt gaacgccgcg cagtgcggct actgcacgcc gggcatcctg atgaccgcca
       61 aggcgctgct ggcggacaac cccgcgccga ccgcggacga gatccgcgag gcgctgtccg
      121 gcaacctctg ccgctgcacc ggctaccaca agatcgtcga ggccgtggag tgggccgccg
      181 cccggatgcg cggcgaggac gggccgccgc cgccgcgcgt cctctatggc gatccgctgc
      241 cggagccggc cggctccccc cggctgcgcg tgctcccttc gttatcggcc gactgaatcc
      301 accgactgag gacacgatac cgatggcgga gcctgtccag aaaccgacgc ccgattccga
      361 gccccggccg acccgggagc cgaacatcat cggcaagccc ttccgccgcg tcgacggccg
      421 ggccaaggtc accggcgcca cccgctacgc cgacgacctg gcgttcccgc gcatggtcta
      481 cctgcggctg gtgcgcgcca cgatccccca cgcgctggtg acccggatcg acttctccga
      541 ggcggagaag gtccccggtg tcctcggctt cctcaccggc cgggacttcc cggtcccgtt
      601 cggcatcctg ccggtgtcgc aggacgagca cgccctctgc ccggacaagg tccgcttcgt
      661 cggcgatccc gtggccgccg tcgccgccgt caccgaggac gcggcgtacg aggcggcgct
      721 caaggtgcgg gtggagtacg agccgctgcc caccatctcg agcgtcgagg aggccctgga
      781 gacgcccgag ccccggatcc acgactacgg cgaccacggc aacgtccaca aggcggtctc
      841 catgaccttc ggcgacgtgg acggcggctt cgccgcggcc ggcgaggtgt tcgaggacgt
      901 cttcttctac gagggcaaca cccacctgcc catggagcag cacgccgccg tggcggtgcc
      961 cgaggacgac gaccgggtga ccctctactc gtccacccag acgccgcact acgtccaccg
     1021 cgcggtgtcc aaggtgctcg gcctccccgc ctcccgcgtg cgggtgatcg cctgctccaa
     1081 cggcggcggc ttcggcggca agagtgatcc gttcaaccac gagatcgtgg cggccaagat
     1141 ggcgctcaag ctcggccggc cggtcaaggt caccctcacc cgcgaggagg tcttctactg
     1201 ccaccgcggc cgccaccccg tgctcatgca ggtgcgcacc ggcatccgca agcacgacga
     1261 cggccgggtc gagatcaccg cccagcacct caagaccgcc ctcgacggcg gcgcctacgg
     1321 cagctacggc gtggcctcga cctactacac cggcgccctg cagacggtga cctacaagct
     1381 gcccgcctac cgtttcgagg gggtgcgctg cttcaccaac aagcccccct gcggcccgaa
     1441 gcgcgggcac ggcacgccgc agccgcgctt cggcttcgag gtgcacctgg acaagatcgc
     1501 ggtcgccacg gggaccaacc cggccgacct gcgcctcggc atgctggccg accccggcac
     1561 ggtgacggcg aactggctgc gcatcggctc gatgggcctg cgccgctgca tcgaggcggt
     1621 ggtcgagggg agcggcttcc gtgagcgctg ggggaagctc cccgagggac gcggcctggg
     1681 cctcgcctgc ggctcctacc tctgcggcgc cggcctcccc atctactgga accacatgcc
     1741 gcagtcgggc gtgcagatca agctcgaccg ctccggcggc gtcgccgtct tctgcggcga
     1801 gtccgagatc ggccaggggt cggactcggt cctcgccgcg gtggtcgccg aggtcctcgg
     1861 cgtgtcgctg tcggacatcc ggctctgcgt ggcggacacc gacctcaccc cggtcgacct
     1921 ggggagctac tcctcgcgcg tgaccctgat gatgggcaac gccgcgcgcg aggcggcgga
     1981 gcgggcgcgg gagatgatcg ccaaggcggt ctcggatcag ctcgaggtcc ccggcgcgcg
     2041 gctggtgttc gccgagaacc gggtgttcga cgccgagaac ccggaggccg gcatgacctt
     2101 ccaggaggcg gtgatcgcca ccgaggcacg cttcggcacc ctggggacca ccggcggcta
     2161 catcccgccg cgctcccccg gccgctaccg gggcgccggc gtcggcccct cccccgccta
     2221 ctcctacagc gccgccgtgg tggaggtgga ggtggacccc gccaccggcc tgtggagccc
     2281 ggtccacgtc tggatggccc acgacatcgg ccgcagcctg aacccggtgc tggtggtcgg
     2341 gcaggtcgag gggagcgtct acatggggct gggcgaggtg atgatggagg agcagtcgtt
     2401 ccggcgcctg ccccggcacc tctcccgcgc cctggtgcac gagttcccgt cgatgctcga
     2461 gtacaagagc ccgaccttcc acgagatgcc gccggtgacc acctacctca tcgaggagcc
     2521 ggaccccgag gggccgttcg gcgccaagga ggtgggccag gggccgctcc tgccgatgat
     2581 gccggcggtg gccaacgcca tccacgacgc cgtgggcgtg cgggtggacc aggtccccat
     2641 ccacccccac atgatcgtca aggcgctggc ggcgaaagcg aaggggggcg agcccagatt
     2701 cggtccccga gcctttcctg atgtagactt cggtgaaaac cttgtcgtcc ccactcccga
     2761 gcaggggggc gacggcaccg cgatcaacga ttaccgcgag aacctccgct ccggcatgcg
     2821 ctcggcctcg ggaacgatga tgtcccgcga ggaagcgttg aagcggaaga aggtcgcggc
     2881 cctgacgacg gatttgaaag gagccaagtg atgagcaacg gatcgaggac cgcgctgaac
     2941 ctgaacgaga cccccctcac cccgctgacc tggggcgaga tgccgcctcc gaccgcccgc
     3001 gcctgatgct gcgcctgcca ccgttccagc tcgtccagcc ccggaccttg cccgaagccg
     3061 ccgccgctct ttccggagag ggcgcggcga acggggagct ggtgcgtctg gtcgccggag
     3121 gcaccgacct ctggcccaac atgaagcggc gccatcagaa ggcgcgggcc gtggtgagcc
     3181 tgatggggat ccccggcctg cgcggggtgg acgccaacgg cgaggtccgg atcggcgcca
     3241 ccaccatcct ggaggatctc gtccgccacg aggcggtgcg gtcgcgctac ccggcgttcg
     3301 cccgggccgt ggcctcgatc tcgtcgccgc cgttgcgcga gatggggacg atcggcggca
     3361 acgtctgcgt cgacacccgt tgcacctact acaaccagac cgaggagtgg cggcgctcca
     3421 tcggctactg catgaaggcc gagggcgaga tctgctgggt cgccaccagc tcgccccgct
     3481 gcctcgccca caccgcctcg gactccgcgc cgatgctctg cgccctgggc gcccgggtcc
     3541 gcctcacctc gcaagagggg
//
LOCUS       DASPJI010000145         4781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_810947, whole genome shotgun sequence.
ACCESSION   DASPJI010000145 DASPJI010000000
VERSION     DASPJI010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810947"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4472
                     /locus_tag="VEL74_03900"
     CDS             <1..4472
                     /locus_tag="VEL74_03900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61701.1"
                     /translation="DVAALRWAMDEIVRRHETLRTTFEEIDGLPMQRIASTGALDLPL
                     VDLAALRGEVAEVRRLASGLALRPFDLARGPLFRGLLVRLGEGDHAVLLALHHVISDG
                     WSIGVFLRELSALYTARLAGRPSPLPSLPVQYADFAVWQRGWLQGKVLARQVGYWREA
                     LAGLAPLELPADRPRPATPGSRGGAVAFSLGEEPSRAVRDLGRQQGATLFMVLLAAFQ
                     ALLQRYTGEDDIAVGTPTAGRNRREIEGLIGFFINMLVLRGDLSGDPGFRALVDQTRE
                     TALGAFAHQDVPFEKVVEELQPARDLGRNPLFQVGFQVAHAPAGGVGLPGLELADVPR
                     ADPAAKVDLNLSFGEGKDGIGGLLEYDADLFEATTVLRLGTHLRRLLESALADPERPL
                     STLPLLTPWEEHQLLAEWNDAAAEEAETSFLQRFEGWTRRTPGATALVFEDEQLTYGE
                     LAERADRLAAALQEMGVGPEVLVGLCLEASLEILVAILAVWKAGGAYLPLDPSYPRDR
                     LAFMLEDSGVSLVLTEERWRHSLPEREALQIINLTHIPSPMGGGASPLGRGEVGSGGG
                     QEGGFSSLDALAYVIYTSGSTGRPKGVAVSRRGLGNLARAHADLFGVGPGGRVLQIAS
                     FSFDASVAKLVGALGTGAALVQARRETWQPGPDLARALARAEVSFSVMVPPLLSAVPP
                     AAASELPALSLLATAGEACPAELVERWAPGRRFWNLYGPTEMTVLCVGGEALPTGKPA
                     DRSPDMGRPFPNCRAHVLDRNLQPVPVGVAGELVLQGPGLARGYLGRPELTAERFVPD
                     PWAALWDEPGARLYRTGDLVRRRAGGRLDFLGRVDQQVKIRGFRIELGEIEAVLREHP
                     EVSAAAATVRQGGAGDRRLVAFAVPRGAPEADRLVREAMDHLRRRLPDYMVPSAVTLL
                     DRLPLTPNGKLDRKALDRIAVGECRGAQGFVPPRTPVEQVLAGIWAEVLKIGRVGAGD
                     DFFALSGNSLLAAQVASRVRQTLGVELPLRRLFERATLGELAREIETARGSRSPEDEV
                     PLPPIERQPRDRPLPASLYQEFGWKLQGGPVGALLNMPQALRIQGPLDLGALLAGLRE
                     MVRRHEELRSVFVETGGGLFLEAGAASRVPLPVIDLSALPAERREGLAHRLAAEEAAR
                     PFDLSRAPGFRAQVLRLGERDHSLLFTMHHLISDGASFAVFRRELWTLAGAFSRGLPS
                     PLPEPPLQPGDFAWWQRRQATAAIDAQVEWWARRLAGRPPALGLPGDRPRPAVVGPEA
                     LADYAVFPAELVAGLRALAQASGCTVGMVLTTAVQVLMHRYTGEVDLVIGTVFAGRNR
                     PELTELISLFLNTLPVRSDLSGRPAFRDLLLQTRDAVLEAYAHQDAPFPRLIAELYPG
                     QAPDRQLLFRVILNLLDFEFAGGPPAEEAGPGDLRVEPMERSEVWATYDFALDVEGGE
                     DFLLCHLTAAADLVTPEGLAAIKLDLVALLEQIAAAPELPLDNLLPEPRHRPV"
     gene            4482..>4781
                     /locus_tag="VEL74_03905"
     CDS             4482..>4781
                     /locus_tag="VEL74_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG61702.1"
                     /translation="MSQQVTQGFRLSPQQRRLWSIQEEDGTSPYRSQCVIRIEGALDR
                     SLLEAAVGDLVARHEILRTLFHRLPGRSWPAQVVLDEEWRPRIELLDLSGREGEVR"
BASE COUNT          549 a         1778 c         1742 g          712 t
ORIGIN      
        1 tcgacgtcgc ggccctgcgc tgggccatgg acgagatcgt ccgccgccac gagacgctcc
       61 gcaccacctt cgaggagatc gacggcctcc ccatgcagag gatcgcatcc accggcgctc
      121 tcgacctgcc gctcgtcgac ctggcggccc tcaggggcga ggtcgccgag gtccgccgcc
      181 tcgcctccgg gctggcgctc cgccccttcg acctggcccg cgggccgctc ttccgcgggc
      241 tcctggtccg cctgggggag ggggaccatg ccgtcctcct cgccctccat cacgtcatct
      301 ccgacggctg gtcgatcggc gtcttcctgc gcgagctgag cgccctctac acggcgcggc
      361 tggcgggaag gccctctcct ctgccgtccc tccctgttca atacgccgac ttcgccgtct
      421 ggcagcgcgg ctggctccag gggaaggtgc tggcgcggca ggtcggctac tggcgggagg
      481 ccctggccgg cctcgcccct ctggagctgc cggccgaccg gccccgcccc gccactcccg
      541 gctcccgggg cggcgccgtg gccttctccc tgggagagga gccgagccgg gcggtccggg
      601 acctcggccg ccagcagggg gcgaccctgt tcatggtcct gctcgccgcc ttccaggccc
      661 tcctccagcg ctacacgggg gaggacgaca tcgccgtcgg cacgcccacc gccggccgca
      721 accggagaga gatcgaaggg ctgatcggct tcttcatcaa catgctggtc ctgcgcggcg
      781 atctgtccgg ggatccgggc ttccgggccc tggtggacca gacgcgcgag accgccctcg
      841 gcgccttcgc tcatcaggac gtgccgttcg agaaggtggt ggaggagctg cagcccgcgc
      901 gggacctcgg acgcaacccc ctgttccagg tgggcttcca ggttgcccat gcccctgccg
      961 gcggcgtcgg cctgccgggt ctggagctcg cggacgtccc gcgcgccgat cccgcggcca
     1021 aggtcgacct gaacctctcc ttcggcgaag ggaaggacgg gatcggcggt ctgctcgagt
     1081 acgacgccga cctcttcgag gcgacgacgg tcctgcgtct cggcacgcac ctccggcggc
     1141 tgctggagag tgccctggcc gatccggagc ggcccctgtc gaccctgccg ctcctcaccc
     1201 cgtgggagga gcatcagctc ctcgccgagt ggaacgacgc cgcggcggag gaggcggaga
     1261 cctccttcct ccagcgcttc gaagggtgga cgcggcggac gccgggggcg acggccctgg
     1321 tcttcgagga cgagcagctc acttacggcg agctggccga gagggcggac cgcctggccg
     1381 cggcgctcca ggagatgggc gtcgggccgg aggtcctggt cgggctctgc ctcgaggcgt
     1441 cgctcgagat cctggtagcg atcctcgcgg tctggaaggc cggcggcgcc tacctccccc
     1501 tcgatccctc gtatccgcgg gaccggctgg ccttcatgct ggaggactcg ggcgtctcgc
     1561 tggtgctgac cgaggagcga tggcgccaca gcctgcctga gcgggaggcg ctgcagatca
     1621 tcaatctgac tcacatcccg tccccaatgg gagggggtgc ctcccccctt gggagggggg
     1681 aggtagggag cgggggaggt caggaggggg gtttttcgtc cctcgacgcc ctcgcctacg
     1741 tcatctacac ctcgggttcc accggccgcc ccaagggcgt ggcggtgagc cggcgcggtc
     1801 tcggcaacct ggcgcgggcg cacgccgatc tgttcggtgt ggggcctgga ggccgggtgc
     1861 tccagatcgc ctccttctcc ttcgacgcct cggtcgccaa gctcgtcggc gcgctgggca
     1921 caggggcggc cctggtccag gcgcggcgcg agacgtggca gccggggccg gacctggcgc
     1981 gggccctcgc ccgcgccgag gtgagcttct ccgtgatggt gccgcccctg ctctccgccg
     2041 tgccgccggc agctgcctcg gagctgccgg cgctctcgct cctcgccacc gcgggggagg
     2101 cgtgcccggc ggagctggtg gagcgctggg ccccggggcg ccgcttctgg aacctctacg
     2161 gccccaccga gatgaccgtc ctctgcgtcg gcggcgaagc cctgcccacg ggcaaacccg
     2221 ccgatcgctc tcccgacatg ggccggccgt tcccgaactg ccgcgcccac gtgctggacc
     2281 ggaacctcca gccggtgccg gtgggcgtcg ccggtgagct ggtcctccag gggccggggc
     2341 tcgcccgcgg ctacctcgga cggccggagc tgacggccga acgcttcgtg cccgacccgt
     2401 gggccgccct ctgggacgag ccgggcgcgc gcctctaccg taccggtgat ctcgtccgcc
     2461 ggcgcgccgg cgggcgcctc gacttcctcg gccgcgtcga ccagcaggtg aagatccgcg
     2521 gtttccgcat cgagctgggg gagatcgagg cggtgctgag ggagcacccg gaggtgagcg
     2581 cggccgcggc gacggtccgc caggggggcg ccggcgaccg ccggctggtg gccttcgccg
     2641 tgccgcgcgg tgctccggag gcggaccgcc tcgtccgcga ggccatggac cacctccgcc
     2701 ggcggctgcc cgactacatg gtgccgtccg ccgtcactct tctcgaccgc ctgccgctca
     2761 ctcccaacgg caagctggac cgcaaggcgc tggaccggat cgccgtcggc gagtgccggg
     2821 gagcgcaggg gttcgtgcca ccgcgcacgc ccgtggagca ggtactggcg gggatctggg
     2881 ccgaggtcct gaagatcggg cgggtggggg ccggcgacga cttctttgcc ctctccggca
     2941 actcgctgct ggcggcgcag gtcgcttcgc gggtccggca gacgctgggg gtcgagctgc
     3001 cgttgcgccg gctgttcgag cgcgccaccc tgggcgagct ggcgcgggag atcgagacgg
     3061 cgcgcggcag ccgctcgccg gaggacgagg tgccgttgcc gccgatcgag cggcagccgc
     3121 gcgatcggcc gctgcccgcg agcctctacc aggagttcgg gtggaagctc cagggcgggc
     3181 cggtcggcgc cctcctcaac atgccccagg cgttgcggat ccagggtccc ctcgatctgg
     3241 gtgccctcct cgcgggcctg cgcgagatgg tgcggcgcca cgaggagctg cgcagcgtct
     3301 tcgtcgagac cggcggcggc ctcttcctgg aggcgggcgc cgcgagccgg gtgccgctgc
     3361 cggtgatcga cctctcggcc ctgccggcgg agcggcgcga gggactcgcg caccgcctcg
     3421 ccgccgagga ggcggcccgc ccgttcgacc tctcccgggc cccgggcttc cgcgcccagg
     3481 tcctgcggct cggcgagcgc gaccactccc tgctcttcac catgcaccac ctgatctcgg
     3541 acggcgcctc cttcgcggtc ttccggcgcg agctgtggac gctggccggc gccttctcgc
     3601 gggggttgcc gtcgccgctc cccgagccgc ccctgcagcc gggcgacttc gcctggtggc
     3661 agcgccggca ggcgacggcg gcgatcgacg cgcaggtcga gtggtgggcg cggcgcctgg
     3721 ccggccgtcc tccggctctc ggcctgccgg gcgaccggcc gcgcccggcg gtggtggggc
     3781 cggaggcgct ggccgactac gccgtcttcc cggcggagct ggtggccggc ctccgcgcgc
     3841 tcgcccaggc gagcggctgc accgtgggca tggtgctgac cacggcggtc caggtcctga
     3901 tgcaccgcta caccggggag gtggacctgg tcatcggcac ggtcttcgcg gggaggaacc
     3961 ggccggagct cacggagctg atcagcctct tcctcaacac gctgccggta cgctccgacc
     4021 tctccgggcg gccggccttc cgggacctgc tgctccagac gcgcgacgca gtgctcgagg
     4081 cctacgcgca tcaggacgcc cccttcccgc gcctgatcgc cgagctctac cccggccagg
     4141 cgcccgaccg ccagctcctc ttccgagtga tcctcaacct gctcgacttc gagttcgcgg
     4201 gcggcccccc ggcggaggag gcgggccccg gggaccttcg ggtcgagccg atggagcggt
     4261 ccgaggtctg ggcgacgtac gacttcgcgc tggatgtcga gggcggagag gacttcctgc
     4321 tctgtcacct gaccgcagcc gccgacctcg tcactccgga ggggctcgcc gccatcaagc
     4381 tggacctggt cgccctcctc gaacagatcg cggctgcgcc cgaactcccc ctggataacc
     4441 ttctccccga gccgcgccac cggccggtct agcgaggtcc gatgtctcag caggttacac
     4501 aaggattccg gctctcgccc cagcagcggc gcctctggtc gattcaggag gaggacggaa
     4561 cgagccccta ccgctcccag tgtgtcatcc ggatcgaggg tgcgctggac cggtcgctcc
     4621 tggaggccgc cgtgggcgat ctggtggcgc ggcacgagat cctgcgcacg ctcttccacc
     4681 ggctgcccgg caggagctgg cccgcccagg tggttctgga cgaggagtgg cggccccgta
     4741 tcgagctgct cgacctctcc ggccgcgagg gggaggttcg g
//
LOCUS       DASPJI010000146         4529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_240385, whole genome shotgun sequence.
ACCESSION   DASPJI010000146 DASPJI010000000
VERSION     DASPJI010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240385"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VEL74_03910"
     CDS             complement(<1..219)
                     /locus_tag="VEL74_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61703.1"
                     /translation="MQSCIHSTTPSVHPNVFTTRFLSRLAERDEPPTAGEADVAGPWR
                     VEPVPVEGFGLFRIGESLDRGFAPVAVFP"
     gene            573..1817
                     /locus_tag="VEL74_03915"
     CDS             573..1817
                     /locus_tag="VEL74_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61704.1"
                     /translation="MNNYNEDDPQVLLYPGGSRAKRIVERAAITGLVGLLATALAVVL
                     CFWKAWWYLLFISIGIWAVVPPLWFAYEYFFIYRQFGLPGTFEIFKHGQQVSGAIWAG
                     ILAALIVLASSDFVKVGSSVGPGCKSQRSDHDFTSAAFAWVPVACANRVEEIESLRSS
                     GEKEDVRESWKRFSETVRSARPGSSVYAPKPYPQSRADILEDFEYAFRSTFVEKTPAG
                     ELPEGVADVYRALERGTLDVEILRVENWGLSRCGGGKLKPFSHLLRLRERESGREIGR
                     MTVHDSGLWGQFRPIPEGADGHASLAIPPLADFQDFLSRRFGFLQPVQKVQYVAADGL
                     RQCSLVVPCVAFQAQGKVYLVESGRLLYEVRLADGLVSVTEHRSRARGPRRLAPDLET
                     PLVSVGFAWAQARRVAGQLAER"
     gene            1859..3121
                     /locus_tag="VEL74_03920"
     CDS             1859..3121
                     /locus_tag="VEL74_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYG61705.1"
                     /translation="MRRAAWGLALLALLLGCGGEGGEERAALVISGSAVGAEGEILAR
                     HVRSFREANPGIPVVVQSTPDDANQRHQLYVQWLNARVGTPDVLQLDVVWTPELAAAG
                     WILPLDRFGPRTEDFFPAALAADTWRDTLYALPWFMDVGMLYWRTDLMEAPPASLQEL
                     AAAARRTGGFVWQGARYEGLVTVYMEVLGAFGGSILTADHRVTVGSPEAVAALVWLRD
                     LIRSGATPREVLTWHEEECRFAFQNGRAAFLRNWPYAVPLLRGPDSAVAGRFAVAPMP
                     AAAGAPGGHPTAALGGAQLAINAYSRRADDAYRLIAWLTAPERMLERAQAAGQYPARR
                     SVYRDPRLAEALGVPAADVLRIVESAAPRPATPLYTQLSEILQIHLHRALSGQTEPGP
                     ALQKAAREMEEVIKRTRIRELEPGAGAQ"
     gene            3121..4017
                     /locus_tag="VEL74_03925"
     CDS             3121..4017
                     /locus_tag="VEL74_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014392991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYG61706.1"
                     /translation="MADPRSDTRLAWGLLAPALVVIAGVALFPLAWTFWESLHLHDLR
                     MPWLGRPFVGLANYAEILGDGRFWSSLGHTLFFAAASVTLELALGLVLALAMDRATRF
                     RGAVRAAVLVPWALPTVVAALLWRFLFDPQAGIVDAALVQAGLVDRPVVWFAHPVTAW
                     VPVILADVWKTTPFVALLLLAGLQGIDRSLYEAAAVDGAGAWRRLVSVTLPLLKPALL
                     VALLFRTLDAFRVFDLVYVLTGGGPGTATEPIALYTFTTFFSNLRFGYGSALSVMVFL
                     VTFGLAMIYIHLLGADPEEGRR"
     gene            4014..>4529
                     /locus_tag="VEL74_03930"
     CDS             4014..>4529
                     /locus_tag="VEL74_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HYG61707.1"
                     /translation="MRNGRWGRWGRWGQWLAAAALVLLLALTAFPLWQALVASVTPEA
                     RLFAGPSAWPSQPVADHYRALWDERDFWKPIRSSLVVAGSTTLICVALGALAAYALAR
                     LRFRGRTVVLGFVLAVGMFPQISIVSPLYLVLRELRLIDTYPGLVLPYVTFALPLAVW
                     LLTGYFRQLPPD"
BASE COUNT          591 a         1520 c         1579 g          839 t
ORIGIN      
        1 cgggaacacc gccacgggag cgaacccccg gtccaggctc tcgccgatcc ggaacaggcc
       61 gaagccctcg accggaaccg gctcgacccg ccacggcccg gcgacatcgg cctcgcccgc
      121 cgtgggaggc tcgtcccgct cggcaagacg gctgagaaaa cgcgtagtga agacgttggg
      181 gtgaacggac ggagtagtac tatggatgca gctctgcata aatcctcctt cgtaggattg
      241 tgtggggtca ggcctggaag tgagtgcccg ctcacttctg ggccgcagcc gggccacgcg
      301 cctggctacc cccggtcatc aagagatgtc gtcgggtgct gcgagtctct tcttccgttt
      361 cgcctcgatc ccctccctcc tcccgggaag acgccacgcc gggtgtgtat ggagcgagtc
      421 tatgagggcg gcaacggggt gtcaagaggg cgggcgaaga ttcttcaagg ttgtttcggg
      481 cgggggggag gggacagggc ggacgacctc ccggaggaga gggggccaac cctaatgccg
      541 tcatcctgag ggacgcagag ccatcatctg ctatgaataa ctacaacgag gacgacccac
      601 aagtactgct gtatccaggt gggagtaggg ccaagaggat tgtcgagcgg gccgccatca
      661 ccggccttgt tggtttgttg gccaccgcgc tcgcggtcgt gctctgcttc tggaaagcct
      721 ggtggtacct tttgttcatc tcgattggca tttgggcagt tgtcccgcct ctgtggtttg
      781 cttatgaata tttcttcatt taccggcagt tcggattgcc tggaacattc gagatcttca
      841 agcatggtca gcaagtctcc ggagctattt gggcgggcat ccttgctgcg ctgatcgttc
      901 tggcatcaag cgactttgtg aaagtaggca gctcagttgg gccgggttgt aagtcgcaga
      961 ggtcagatca cgacttcacc agtgcagcct tcgcctgggt tcccgtggcc tgcgcgaacc
     1021 gtgtcgagga gatcgagagc cttcgctcca gcggagagaa ggaggatgtg agggagagtt
     1081 ggaagcggtt ctcggagacc gtccggagcg cgaggccggg atcgtccgtc tatgcaccca
     1141 agccctatcc tcagagccga gccgatatcc tggaagattt cgagtacgcc ttccggagca
     1201 ctttcgttga gaagactccg gccggtgagc ttccggaggg agtggcggac gtctatcgtg
     1261 ccttggagag agggacgctg gatgtcgaga tcctgcgggt cgagaactgg ggcttgagcc
     1321 ggtgcggcgg cggcaagctc aagccttttt ctcatctcct tcgacttcgc gagcgcgaga
     1381 gcggacgcga gatcgggcgg atgaccgttc acgactcagg gctgtggggc cagtttcggc
     1441 cgattccgga aggcgccgac gggcatgcga gtctggccat cccgcctctg gctgatttcc
     1501 aggatttcct gagccgccga ttcggtttcc tgcagcctgt ccaaaaggtc cagtatgtcg
     1561 ccgctgatgg cctgaggcag tgctcgcttg tcgtcccctg tgtcgctttc caggcgcagg
     1621 gcaaggtgta cctcgtggag tccggccgtc tcctctatga ggtaaggctg gcggacgggc
     1681 tggtctcggt cacggagcac cggtcgcgag cccgtggacc gaggaggctt gcccccgatc
     1741 tcgagactcc cctggtcagc gtgggcttcg cctgggctca ggcccggcgg gtggcggggc
     1801 agctcgcgga gaggtagcgg aacccctccg gccccgatcc tgctagcctc ccagggtgat
     1861 gcgtcgcgcc gcctggggtc tcgcgctgct ggccctgctc ctcggttgtg gtggggaagg
     1921 tggggaggag cgcgccgctc ttgtcatctc cggcagcgcg gtcggggccg agggggagat
     1981 cctcgcccgg catgtccggt cgttcaggga ggcgaacccc ggcattccgg tggtcgtcca
     2041 gagcacgccg gacgacgcca atcaacggca ccagctctat gtccagtggc tcaacgcccg
     2101 ggtggggacg ccggacgtcc tgcaactcga cgtggtctgg acgcccgagc tcgccgcggc
     2161 gggttggatc ctccccctgg accgcttcgg cccgcgcacc gaggacttct ttcccgccgc
     2221 cctggccgcc gacacctggc gggacaccct ctatgccctg ccatggttca tggacgtggg
     2281 gatgctctac tggcggaccg acctcatgga ggcccctccc gcttctctcc aggagctggc
     2341 ggcggcggcg cggcggacgg gtgggttcgt ctggcagggg gcccgctacg aggggctggt
     2401 gacggtctat atggaggtgc tgggcgcgtt cggcgggagc atcctgacag cggaccatcg
     2461 ggttacggta ggctcgcccg aggccgtggc cgccctggtc tggctgcggg acctgatccg
     2521 ctccggcgct acgccgcggg aggtgctcac ctggcacgag gaggagtgcc ggttcgcctt
     2581 ccagaacggg cgcgccgcct tcctccgcaa ctggccctat gccgtgccgc tcctgcgcgg
     2641 gccggactcc gccgtggccg gccgcttcgc ggtggcgccc atgccggccg cggccggcgc
     2701 gccgggcggc catcccacgg cggccctggg cggggcccaa ctcgccatca acgcctacag
     2761 ccggcgggcc gacgacgcct atcgcctcat cgcctggctc accgcgccgg agcggatgct
     2821 ggagcgggcc caggcggccg gccagtaccc ggcccggcgc tcggtctacc gggacccgcg
     2881 gctcgccgag gctctgggcg tcccggcggc cgacgtgctg cgcatcgtcg agtcggcggc
     2941 gccgcggccg gcgactcctc tctatacgca gctctcggag atcctccaga tccatctcca
     3001 ccgggcgctc tcgggtcaga ccgagccggg cccggcgctc cagaaggcgg ctcgcgagat
     3061 ggaggaggtc atcaagcgca cccgcatccg cgagttggag cccggtgctg gagcccagta
     3121 atggctgatc cccggtcgga tacccgtctc gcctgggggc ttctcgcccc ggccctcgtg
     3181 gtgatcgccg gcgtggccct cttccccctg gcctggacct tctgggagtc gctccacctc
     3241 cacgatctgc gcatgccctg gctgggccgc ccgttcgtcg gcctggccaa ctacgccgag
     3301 atcctggggg acggccgctt ctggagctcg ctggggcaca ccctcttctt cgccgccgcg
     3361 agcgtcactc tcgagctggc gctgggactc gtgctcgctc tcgccatgga ccgggcgacc
     3421 cgattccgtg gcgcggtgcg ggcggcggtg ctggtgccct gggcgctgcc cacggtggtg
     3481 gcggcgctcc tctggcgctt tctcttcgac ccccaggccg ggattgtgga cgccgcgctg
     3541 gtccaggccg gcctcgtgga ccgcccagtc gtctggttcg cccacccggt gaccgcgtgg
     3601 gtgccggtaa tcctggccga cgtctggaag accaccccct tcgtggccct gctgctcctc
     3661 gccggcctgc agggcatcga ccgctcgctc tacgaggcgg cggcggtgga cggggcaggg
     3721 gcgtggcggc ggctcgtgtc cgtcaccctg ccgctgttga agccggcgct cctcgtggcg
     3781 ctcctcttcc ggaccctgga cgccttccgg gtcttcgacc ttgtctacgt gctgaccggc
     3841 ggcggcccgg gcaccgccac cgagccgatc gccctctata ccttcaccac cttcttctcc
     3901 aacctgcgct tcgggtacgg ctcggcgctc tcggtgatgg tcttcctggt gaccttcggc
     3961 ctggcgatga tctacatcca tctcctgggc gcggacccgg aggaggggag gcgatgagga
     4021 acggccgatg gggccgatgg ggccgatggg gccaatggct ggccgccgcg gcgctggtcc
     4081 ttctcctggc gctgaccgcc ttccccctct ggcaggcgct ggtggcgtcg gtcactcccg
     4141 aggcgcggct cttcgccggc ccctcggcct ggccgtccca gccggtggcg gaccactacc
     4201 gcgcgctgtg ggacgagcgg gacttctgga agccgatccg gagctcgctg gtggtggccg
     4261 gctccaccac cctgatctgc gtcgccctcg gggcgctggc ggcctacgcc ctggcgcggc
     4321 tgcgcttccg gggccggacc gtggtcctgg ggttcgtgct ggcggtgggg atgttcccgc
     4381 agatctccat cgtcagcccc ctctacctgg tgctgcggga gctgcggctg atcgatacct
     4441 acccggggct cgtgctgccg tacgtgacct ttgccctgcc gctggcggtg tggctcctca
     4501 ccggctactt ccggcagctc ccgcccgat
//
LOCUS       DASPJI010000147         5673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_69315, whole genome shotgun sequence.
ACCESSION   DASPJI010000147 DASPJI010000000
VERSION     DASPJI010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69315"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>5673
                     /locus_tag="VEL74_03935"
     CDS             <1..>5673
                     /locus_tag="VEL74_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61708.1"
                     /translation="AVEAPPILPVPRQGSLPLSFAQQRLWFLHRLAPDSAAYNVPLTV
                     GLSGRLDAAALAAALHEIGRRHEVLRTTFAERQGRPFQVIAPEPRLPLPLLDLSGLPA
                     AEREAEVLRRALTEALAPFDLAAGPLVRAALLRLGEEDHALLVTMHHIVTDGWSRGIF
                     LRELTALYAGHRLPELPVQYGDFAVWQRAWLSGGVLETQISYWRQRLAGAPARLALPA
                     DRTRPAVATDRGELRPVALSPELSRSLTALARSEGATPFMVLLAGFAALLGRYSGQDD
                     VVVGTPVAGRNRREVEGLIGFFVNNLALRVELADGLGFVDLLRQVRRTALDAYAHQDI
                     PFERLVEEVVAERGGLDHSPLFQVTLAVQNTPVVGDGVVPGLELRRVTAEPVAAKFEL
                     SLVFALLGEELAGGLEYNTDLFDATTADRLARHFARLLEVAMAAPEMAWPELPLLDRS
                     EAQQVLLEWNDTEAAYPGSGSCLHELVFEQAERTPEAVAVVAGEEGEEHLTHAELRRR
                     AGWLAHRLRRLGVAPEVPVALCAERSLEMVVGLLGILEAGGAYVPLDPDYPAERLAYM
                     VEDSAAALLLAQRHHTDRLPENGTRILFLDELACPEAGAPPAMPDGATPDNLLYVIYT
                     SGSTGRPKGVMVRHRGVVNRLLWAAHAYPVTAADRVLQKAPFSFDFSVWECFGALLSG
                     ARLVLARPGGQRDGAYLARTVVEQGITLLHFVPAMLQAFLEEEGLESCHSLRFIFSGG
                     DALTSETVERFQVRMPAGVLLRNQYGPTEISIDVTEWVCREERTGSTPPIGKPLGNGR
                     VYVIGPNGELVPAGVPGELLLGGVGVARGYRGRPDLTAERFVPSPWGDEAGARLYRTG
                     DRVRQLADGNLEFLGRVDHQVKIRGYRIEPGEIEAVLALHPAVREAAVVVRQDAAGLR
                     SLLACVVPVPLPGTAAVTTEELRAAMRGSLPDYMVPGAFALLEELPLTPSGKVDRKAL
                     EKLAPARDERAAEPAAIRPRTPSEEILAAIFAEVLRHERVGLHDGFFDLGGHSLLATQ
                     VVSRVRQAFGVELPVRELFEAPTVAGLAERIERLRRARSELAVVPVPRVPGEQGGLPL
                     SFAQQRLWFLHRLAPDSAVYNVPMAVRLSGRLDAAALAGALREIGRRHEALRTTFSEL
                     QGHPVQVIDPEPRLTLMTVGLADLPPSEREAEVLRRALAEALAPFDLAAGPLVRATLL
                     RLGAEEHALLVTMHHIVTDAWSMGIFLRELTTLYGAFVQDRPSPLPELPIQYGDFAAW
                     QRIWLSGEVLESQISYWRERLSGVPVRLNLHTDRPRPAVETHRGASLPVVLPLELSRS
                     LTTLARREGATPFMVLLAGFAALLGRYSGQDDVVVGSPIAGRNRREIEGLIGFFVNTL
                     ALRVELADGLGFMELVRQVRQTALDAYAHQDIPFERLVEELVTERDLSHSPLFQAVLT
                     LQNASVITEGGGVPGLELRPVEVAPGTAKFELSLGFTLRGEELAGSLEYNTDLFDTAT
                     VERLARHLARLLEGATASPGVWLAELPLLDESERRQLLQEWGQGLATPATDRPVHRLV
                     AARAAEAPEAVALVSAAGHAVTYGELSRRAAGLAHRLRALGVGPEERVGVLLDRSPEE
                     VTSLLAVLLAGGAYVPLDPTWPRERVGLVIEDAGVRVVLTTSERGDLLPGPSPLLLDR
                     VWDEAGSATSGPPAEVDHLAYVIYTSGSTGRPKGVEITHRSLLRLLAWHQRAFAVTAA
                     DRATRLSGLAFDAAVWELWPYLVSGASVHVPDEESRTSPPALREWLLAQGITIGFVST
                     PVAEALLPISWPAYTPLRVLLTGGDRLHRDPGPLPFTLVNNYGPTESTVVATSGAVQP
                     QGAGRAPDIGQPVDGTRVLLLDRELRLVPPGVAGEICIGGPGLARGYLG"
BASE COUNT          673 a         1881 c         2227 g          892 t
ORIGIN      
        1 ggcggtggag gcgccgccga tcctgccggt gccgcgccag ggtagcctgc cgctctcgtt
       61 cgcccagcag cggctctggt tcctccaccg gttggcgccg gatagcgcgg cctacaacgt
      121 gcctctgacg gtggggctgt cgggccggct ggatgccgcc gcgcttgccg cagcgctcca
      181 cgagatcggc cgccgtcacg aggtgctgcg caccaccttc gccgagcgcc agggccgccc
      241 tttccaggtg atcgcgccgg agccgcggct gccgctgccg ctcctcgatc tctcgggcct
      301 gccggcggcg gagcgcgagg ccgaggtgct gcggcgcgct ctgaccgagg cgctggcgcc
      361 gttcgatctc gcggccgggc cgctggtgcg cgcggcgctc ctgcggctgg gcgaggagga
      421 ccacgcgctg ctggtgacca tgcaccacat cgtcaccgac ggatggtcca ggggaatctt
      481 cctgcgcgag ctgacggcgc tctatgccgg tcaccggttg ccggagctgc cggtgcagta
      541 cggcgacttc gcggtctggc agcgggcctg gctgtcgggc ggtgtgctgg agacgcagat
      601 ctcctattgg cggcagcgcc tggccggagc gcccgcgcgt ctcgccctgc ccgcggaccg
      661 gacgcggccg gcggtggcga ccgaccgggg ggagttgcgg cccgtggccc tgtctcctga
      721 gctgtcgcgg tcactcacgg cgctggcccg gagcgaaggg gcgacgccgt ttatggtcct
      781 gctggccggg ttcgcggctc tcctgggccg ctactccggc caggacgacg tggtggtggg
      841 gacccccgtc gcgggccgca accggcggga ggtcgaaggg ctcatcggct tcttcgtcaa
      901 caacctggcg ttgcgagtgg agctggcgga cgggctcggc ttcgtggacc tgctccggca
      961 ggtgcggcgg acggcgctgg acgcctacgc gcaccaggac atccctttcg agcgcctcgt
     1021 cgaggaggtg gtggcggagc ggggcggcct cgatcactcg ccgctctttc aggtgacgct
     1081 ggcggtgcag aacacacccg ttgtcggcga cggggtggtg ccggggctgg agctgcggcg
     1141 ggtgacggcg gagcccgtcg cagccaagtt cgaactgagc ctggtcttcg ctctgctcgg
     1201 cgaggagttg gcgggcggcc tggaatacaa caccgacctc ttcgacgcca ccacggcgga
     1261 ccggctggcg cggcacttcg cgcggctgct ggaggtggcg atggcggcac cggagatggc
     1321 ctggccggag ctgccgctgc tggaccggtc cgaggcgcag caggtgctgc tggagtggaa
     1381 cgacaccgaa gctgcctatc cgggaagcgg gagctgtctg catgaattgg tcttcgagca
     1441 ggcggagcgg acgcctgagg cggtggcggt ggtggccggc gaggaggggg aggagcacct
     1501 gacccacgcc gagctgcgcc gccgtgcggg gtggctggct catcggctgc ggcgcctcgg
     1561 ggtcgcgccg gaggtgcccg tggcgctctg cgccgagcgc tcgctggaga tggtggtagg
     1621 cctgctgggc atcctggagg cgggcggcgc gtacgtcccc ctggaccccg actatccggc
     1681 cgagcgcctg gcctacatgg tggaggactc ggcggcggcg ctgctgctgg cccagcgcca
     1741 ccacacggac aggctgccgg agaacgggac tcggatcctc ttcctggacg agctggcgtg
     1801 tccggaggcg ggcgcgccgc cggccatgcc cgatggcgcc acgccggaca acctgctgta
     1861 cgtgatctac acctcgggct cgacgggccg ccccaagggg gtgatggtgc gccaccgggg
     1921 cgtggtcaac cgcctgctct gggcggccca cgcctatccg gtgacggcgg cggaccgggt
     1981 gctgcagaag gcgccgttca gcttcgactt ctcggtctgg gagtgcttcg gcgcgctgct
     2041 gtccggggcg cggctggtgc tcgcccgccc gggcggccag cgcgacggcg cctacctggc
     2101 gcggacggtg gtggagcagg ggatcacgct gctgcacttc gtgccggcga tgctgcaggc
     2161 cttcctggaa gaggaggggc tggagagctg ccacagcctg cgcttcatct tctcgggcgg
     2221 cgatgccctg acgtccgaga cggtggagcg gttccaggtc cggatgcctg ccggggtgct
     2281 gctgcgcaac cagtacggcc ccaccgagat ctccatcgac gtcaccgagt gggtctgccg
     2341 tgaggagcgg accggcagca cgccgcccat cggcaagcct ctgggtaatg gccgggttta
     2401 tgtcatcggg cccaatgggg agctggtgcc ggccggggtg cccggagagc tattgctggg
     2461 cggcgtgggg gtggcccgtg gctaccgggg ccggcccgac ctgacggcgg agcggttcgt
     2521 gccctccccc tggggagacg aggcgggggc gcgtctctat cgcaccgggg accgggtgcg
     2581 ccagctggcg gacggcaatc tcgagttcct gggccgcgtc gatcaccagg tcaagatccg
     2641 cggctaccgc atcgagccgg gggagatcga agcggtgctg gcgctgcatc ctgcggtgcg
     2701 cgaggcggcc gtggtggtgc ggcaggacgc ggcggggctg cggtcgctgc tggcctgcgt
     2761 ggtgccggtg ccgctgccgg gcaccgccgc agtgaccacc gaggagctgc gggccgcgat
     2821 gcgcggctcc ctgccggact acatggtgcc cggagcgttc gcgctcctgg aggagctgcc
     2881 gctgacgccg agcggcaagg tggaccgcaa ggctctggag aagttggctc cggcgcgcga
     2941 cgagcgcgcc gcagagccgg cggcgatcag gccgcgcacg ccgtccgagg agatcctggc
     3001 ggcgatcttc gcggaggtcc tgcggcacga gcgggtggga ttgcacgacg gcttctttga
     3061 tctcgggggg cactcgctgc tggcgaccca ggtggtgtcg cgggtacgcc aggcctttgg
     3121 ggtcgagctg ccggtccgcg agctcttcga ggcgccgaca gtggccggct tggccgagcg
     3181 gatcgagcgg ctgcggcgag cccgctccga gctggcggtc gttcccgtgc cgcgggtgcc
     3241 cggagagcag gggggcctgc cgctctcctt cgctcagcag cggctctggt tcctccaccg
     3301 gctggcgccg gacagcgcgg tctacaacgt gccgatggcc gtgcggctgt cgggccggct
     3361 ggacgcagcc gcgcttgccg gcgcgctccg cgagatcggc cgccgccacg aggcgctgcg
     3421 caccaccttc tccgagctcc aggggcaccc ggtgcaggtg atcgacccgg agccacggtt
     3481 gactctgatg accgtcggtc tcgcggacct gccgccgtcg gagcgcgagg ccgaggtcct
     3541 tcggcgagcc ctggccgagg cgctggcgcc attcgatctc gcggccgggc cgctggtgcg
     3601 cgcgacgctt ctgcggctgg gtgctgagga gcacgcgctg ctggtgacca tgcaccatat
     3661 cgtcaccgat gcatggtcca tgggcatctt tctgcgcgag ctgacgacgc tgtacggcgc
     3721 ctttgtccag gatcggcctt cgccgttgcc ggagttgccg atccagtatg gcgatttcgc
     3781 ggcctggcag cggatctggc tgtcgggaga ggtgttggag tcgcagatct cctactggcg
     3841 ggagcggttg tcaggggtgc cggtccggct caacctacac acggaccggc cgcggccggc
     3901 ggtggagacc caccgtgggg cttccctgcc ggtggtgctg cctctggagc tgtcacggtc
     3961 gctcacgacg ctggcccggc gcgagggggc gacgccgttc atggtcttgc tggccgggtt
     4021 cgcggccctc ctgggccgct actccggcca ggacgacgtg gtggtgggtt cgcccatcgc
     4081 gggccgcaac cggcgggaga tcgaggggct gatcggcttc ttcgtcaaca ccctggccct
     4141 gcgggtggag ctggcggacg gcttgggctt catggagctg gtgcgtcagg tgcggcagac
     4201 ggcgctggac gcctacgcgc atcaggacat ccccttcgag cgtctggtcg aggagctggt
     4261 gacggaacgg gacctgagcc actcaccgct gttccaggcg gtgctgacgc tgcagaacgc
     4321 gtccgtgatc acagaaggcg gtggggtgcc ggggctggag ctgcggccgg tggaggtggc
     4381 gcccgggaca gcgaagttcg agctgagcct gggctttact ctgcgcggcg aggagctggc
     4441 agggagcctg gagtacaaca ccgacctgtt cgatacggct acggtggagc gtctcgcccg
     4501 gcacctggcg cgcctgctgg aaggggctac cgccagtccg ggggtctggc tcgcggagct
     4561 gccgttgctg gatgagtccg aacggcggca gctcctccag gagtggggcc aggggctggc
     4621 gacgcccgcg accgaccggc cggtgcaccg gctggtggcg gcgcgagcgg ccgaagcgcc
     4681 ggaggcggtg gcgctggtct cggcggccgg ccatgccgtg acctacggcg agctgagccg
     4741 gcgcgcggcg gggctcgctc accgtctgcg cgccctggga gtggggccgg aggagcgggt
     4801 gggagtcctt ctggaccgct ctccggagga ggtcacgagc ctcctcgcgg tgctcctggc
     4861 cgggggcgcc tatgtgccgc tcgatccgac ctggccgcgg gagcgggtgg ggctggtgat
     4921 cgaagacgcc ggcgtccggg tggtgctgac cacctcggag cggggagacc tcctgccagg
     4981 tccgagcccg ctgctgctgg atcgcgtctg ggacgaggcg ggctcggcga cctccggacc
     5041 tcccgcggag gtcgaccatc tggcctacgt gatctacacc tcgggctcga ccggcagacc
     5101 caaaggagta gagatcactc accggagcct gctgcgtctg ctcgcctggc accagcgggc
     5161 cttcgcggtg acggctgcgg accgggcgac tcggctgtcg ggcctggcct tcgatgccgc
     5221 ggtctgggag ctttggccct atctcgtgtc cggcgccagt gtccacgtac cggacgagga
     5281 gagccgcacc tcccctccgg ctctgcgcga gtggctgctc gcccagggga tcaccatcgg
     5341 cttcgtctcc acgccggtgg ccgaggctct gctgcctatt tcctggcctg cttacacccc
     5401 cctgcgggtg ctgctgaccg gtggcgaccg tctgcaccgc gaccctggcc cgctgccgtt
     5461 cacgctggtc aacaactacg gcccgacgga gagcacggtg gtggcgacct cgggcgcggt
     5521 gcagccgcag ggcgcgggcc gggcaccgga catcggccag ccggtcgacg gcacccgggt
     5581 gctgctgctg gaccgggagc tgcggctggt gccgcccggg gtggccgggg agatctgcat
     5641 cggcggcccg ggcctggccc ggggctacct ggg
//
LOCUS       DASPJI010000148        12434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_811007, whole genome shotgun sequence.
ACCESSION   DASPJI010000148 DASPJI010000000
VERSION     DASPJI010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12434)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12434
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811007"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VEL74_03940"
     CDS             complement(<1..478)
                     /locus_tag="VEL74_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61709.1"
                     /translation="MPEFHQYIGVRYSGRKEPGDRIREIRVFSAVEDHEPYKEGNPSH
                     EAGWWSRQELAEWLLEKLTGGEPVVVGIDHAFSFPQSYMDRHNLKSWGDFLEDFEGHW
                     PTDRFSVRELLPGNPRTGDPDEHRLTNRWTASPKSVFQFDIQDSLAKSTHAGLPWLD"
     gene            765..1535
                     /locus_tag="VEL74_03945"
     CDS             765..1535
                     /locus_tag="VEL74_03945"
                     /inference="COORDINATES: protein motif:HMM:NF044375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiJ family protein"
                     /protein_id="HYG61710.1"
                     /translation="MIEFLLANGNLPFTIALTVMVGIALLEAAGTLLGFGSSAAHPAG
                     ADLDAGGGPHLHLDGHADVHVDHPELHAGHHGALAHADHGDLHHDVHGDADSASAIGK
                     LLAWLGLGKLPAMVWLVVFLTSFGLTGLALQMLIRGFAGGLLPAGAAAPMALFLCLPV
                     VKVGGGLLARILPRDETSAVAAESFVGQVAVVTLGTARRGQPAEAKLRDTYGKTHYVL
                     VEPESSGDELETGSQVLLVSRAGAVFSGVRNPYGTLMD"
     gene            1567..3384
                     /locus_tag="VEL74_03950"
     CDS             1567..3384
                     /locus_tag="VEL74_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009427444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
                     /protein_id="HYG61711.1"
                     /translation="MENLIFIAFVAGMALIALLAIGFILTRLYHRASKEESFVRTGFG
                     GQKVILNGGALILPVLHETIRVNMNTLRLEVRRSNEQALITRDRMRVDVQAEFYVRVK
                     PTEEAIADAAQTLGKRTMDPNALKELVEAKFVDALRAVAAEMAMQELHEQRVNFVQKV
                     QTAVSEDLLKNGLELESVSLTSLDQTNREYFNPNNAFDAEGLTKLTEEIETRRKKRND
                     IEQDTQVAVQSKNLEAERQKLELSKQEEYVRLDQQREIAIRRAAQAAEIAREQAEKNR
                     QAKEAEILAKQQVDLADISAHQALAQQRIETERQVQELDIVKVRTLETAEIEKRKAVE
                     MAEQDRAIAIAEKSKAQSEAQALADQARAVAAKAEEQVVTAREAERAERAKQIELVKA
                     SQEAEREAIGITTGARAEKQAAEDRATAVRTLAHAEADQAKIAAQGEAEAEVVRAEAA
                     AKRYEVEATGKRALNEAANALSPEAIAMQVRLALLEHLPEIIREAVKPIERIEGIKIV
                     QVGGLGLDGAANGDGQVVTGGGGGGSLADQVVNSALRYRAQAPLLDSLLQDVGLSSHE
                     LAGLTQALHAKGNGGAEPAHPQLPQGETVAVHKPKQKTT"
     gene            3432..5699
                     /locus_tag="VEL74_03955"
     CDS             3432..5699
                     /locus_tag="VEL74_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HYG61712.1"
                     /translation="MPRYTLPMSTFLIRAARRRNLIPRISETERQALEAGTVWVDGEL
                     FSGRPDFRRMLREPYPILADEERAFLDGPVERLCALVDDWEVSRRRELPPKVWDLLKE
                     ERFFGLAIPREHGGHGFSPLALSTIFGTLASRSLALSAVVLIPNSVGPGELLLEYGTP
                     EQQAFFLPRLARGEEIPCFALTEPEAGSDAASLRSEGVLFRDSKGDLAIRLSWSKRYI
                     TLAPVATLLGLAFRLRDPENLLGRGEDVGITLALVPTHLPGVRIGDRHDPMGVPFPNG
                     PTEGRDVVIPADRIIGGPEYAGRGWKMLMEALSAGRSISLPAQSVGGAKYVARVAGAY
                     AAVRRQFGVEIGRFEGIEEPLARIGGLTWLMEAARVATCGAVGSGHRPAVISGVVKYQ
                     QTELLRRVVQDGMDVLGGAALSRGPRNLMAAAHMGAPIGITVEGANILTRTLIVFGQG
                     AIRCHPYALAEIRALESGDAAALRKALLGHAGSFLRNLVRSALLGLTRGRLAGSPVAG
                     PTAGSWRRLAWASARFALLTDLALLSLGSRLKLREKLAGRFADALSWMYLGFCALRRF
                     EAEGRREEDLPFVLWATEHALDQIQTAFEGIYRNFEGPLGWLMRGPGAWWCRVNPLGR
                     PPSDRRGARVAAALRSAGALRDRLTAGVFLPADPTQALGRLERAFALVAEAAPVAETI
                     ARASRQGTLPAGRPEELLDWAVAAGVVTAEQAGLVRRAAEARLDAIQVDSFGLEEYLR
                     LERPKAAPAEAVQLS"
     gene            complement(5692..6993)
                     /locus_tag="VEL74_03960"
     CDS             complement(5692..6993)
                     /locus_tag="VEL74_03960"
                     /inference="COORDINATES: protein motif:HMM:NF023252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3344 domain-containing protein"
                     /protein_id="HYG61713.1"
                     /translation="MRVSVVKLCLAVLCMVLLGGTVQATTLSVALDIQGQGLAMTEGG
                     VGLQAIGSGTRNLTVNIPGTVQAALLYWTGRDRPCPMSGGSCIIPSQPYKDQVLRFDG
                     SLITGTIIGTETQPASAGGPINNIGFLADVTSIVQARGTGSQTFPIQDGNTASNLFHL
                     NGAGLVVIYTNPADTAMYRLIVYDGLDFAYGIDPTPGPTRVTVPVTFSHGSSTSARSA
                     TLWVFSGDGTPNRPDRIDISNNPSVVNGLNASDGASWDTDAITVNVPAGVSSTTVQLF
                     SEPLNQNPDSLLWILGALRIPLPVVTPPPPPPVTGDEGCTPGYWKNHTESWAGTGYTP
                     GQTVGSVFSAGGFPSLGSQTLLQALQGGGGPGSLGAAKILLRAAVAALLNAAHSGVDY
                     TRTASAIISDVNAALASNNRDTMLALATRLDNDNNLGCPLN"
     regulatory      complement(7120..7195)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            7351..9837
                     /locus_tag="VEL74_03965"
     CDS             7351..9837
                     /locus_tag="VEL74_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYG61714.1"
                     /translation="MPLFRVLAALLLLPVCVAVPVAAATGSSTDPVYRTPPQALVDIV
                     DAPPTPDVDVDPRREWLLLLERPNLPPISELAERELRLAGLRIRPRTSGPSRTRYFTG
                     LELLRVADGSRRRITGLPTGARIENIRWSPDGSRLAFTHTGQEAIELWVAEVATGQAR
                     RLGDARLNLVARTVPVWLRDGRGLVATLVPADRGAEPSVSQVPTGPVIQESLGRTAPA
                     RTYQDLLKSASDEALFEHYLTSQLARIDLDGRVTPLGAPGLLDRFSTSPDGRYLLTET
                     LHRPFSYLVPASRFPRRIEVWDLATGQRVREIADVPLQDEVSTQFDAVPKGPRQLEWR
                     ADAPATLVWAEALDGGNPSAPAEHRDRIVALAAPFVGDPVILATLPLRYSGVSWGNGD
                     TALVRDRWWKDRRIRTWRIRPDSPTSAPDLLFDRSSEDRYSDPGVPVMVENEAGRHVM
                     LLAGGGKTLYLTGDGASPEGDRPFLDAYDLTTRKARRLFRSEAPFYEDPLLPLPSRDN
                     AVRELLVRRESVMEPPNYFVRDLRTGKLRQLTSFPHPMPQLAGLQKELVRYKRDDGVQ
                     LTATLYLPPGYKPADGPLPLLMWAYPEEFKSADAASQVQDSPYRFDRVAPGSPLVWLA
                     AGYAVLDNPTMPIVGAGDAEPNDTYVEQLVASARAAVDEMVRRGVADPRRIAISGHSY
                     GAFMTANLLAHSDLFAAGIARSGAYNRTLTPFGFQAEERLLWKASETYLRMSPFMYAD
                     KINEPILLIHGQVDNNSGTDPIQSERFYSALKGQGATTRLVMLPGESHGYAARESLLH
                     MLWETHSWLERYVKNRPAEVVSSSASKAPR"
     gene            9873..10031
                     /locus_tag="VEL74_03970"
     CDS             9873..10031
                     /locus_tag="VEL74_03970"
                     /inference="COORDINATES: protein motif:HMM:NF033521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso RiPP family leader peptide-containing
                     protein"
                     /protein_id="HYG61715.1"
                     /translation="MTYEAPEVFELGSAEELTLGSCGGPMDWCGFYIIDIQGCEPPCG
                     VHEEPREE"
     gene            10088..10822
                     /locus_tag="VEL74_03975"
     CDS             10088..10822
                     /locus_tag="VEL74_03975"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HYG61716.1"
                     /translation="MDGRFEAAEHVRSSTGEEGTVLLDLRSGKYFALNAVGGLLWESM
                     AAGASRSVLVQRLAERFPEVPADRLGHDADALLSQLLAKGLLRPRTGPGDEKEAPAPR
                     PVFQPVWKDGSALEAATASPFWVPIAFLALLASDFILQVLGFSRFHGLVRRIPTRPAA
                     ASAARAQRIVRSVDRASAFYFKRAWCLQRSAVTAGLLRLSGLPARLVIGARRVPFYAH
                     AWVELDGRVVNDRPAVRQEYEVLETC"
     gene            10977..>12434
                     /locus_tag="VEL74_03980"
     CDS             10977..>12434
                     /locus_tag="VEL74_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HYG61717.1"
                     /translation="MAHASFHTAPGPPAEQPCRHGAVLVTWDGRLDNRADLAASLGAE
                     RANDVELLALAYERWGPTFPSRIVGDFALALWDGRARLLLLARDPVGTRPLFYAVRPD
                     GGLLWASTLAALRAGGADLGEVDEEWIAGYFSWSIDPATTPWRGVRAVPPGHVLAVGG
                     GPPRLQCFWKAEEVPAVRLGGDAEYEERYRELFLASVQNRLRAAGPVAAELSGGVDSS
                     SIVCAAAHLLRAGSAETPGLLTASHLYERSRTADETPFIEEVEAFIGRTGRHILESEA
                     PILTGLVTDIPEIPTSNHCFRRRYQAIYDFMAENGSRVLLSGLGGDHLLTSQVATPYV
                     LADLLRGGRLGALRTGLAAWRRELGKPYLQLLWEGAVCPLLPRPARMRLARPPIPFPS
                     WLDRGFVRRRDLAARFVALTDAEGYPGPPGKREQAGAVRGALRAAAWLYDWWGRPVEV
                     TFPFLDRPLMEFCLGAPAGQFVRPGETRSLHRRALA"
BASE COUNT         1788 a         4582 c         4178 g         1886 t
ORIGIN      
        1 agtccagcca ggggaggccc gcgtgggtcg acttcgccag gctgtcctgg atgtcgaact
       61 ggaagacgct cttcggcgac gccgtccagc ggttggtcag gcggtgctca tcggggtcgc
      121 cggtccgggg gttacccggc agcagctcgc gtaccgagaa gcggtccgtc ggccagtgcc
      181 cctcgaagtc ctccaggaag tccccccagc tcttcaggtt gtggcggtcc atgtaggact
      241 gcgggaacga gaaggcgtga tcgatgccga ccaccaccgg ctcgcctcca gtcagcttct
      301 cgagcagcca ctcggccagc tcctggcggc tccaccagcc cgcctcgtgg ctcgggttcc
      361 cttccttgta gggctcgtga tcctccaccg ccgagaagac ccggatctcg cggatgcggt
      421 cccccggttc cttgcggccg gagtagcgga caccgatgta ttgatggaac tcaggcatgc
      481 atccctcccg ttctcgcgca cagtctatca atcaagtgcc cgtcatccgg gtggccgagc
      541 ctccctcccc caaagaggcc catgaaacgg aatggggcgg atcgacacct atggatggaa
      601 acgggcccgc cgtctgtttg gggacccgca accggataga tcttggaccc tgacatagaa
      661 tccttcctga cgaagcgacc ctgccgagag acagaccggc cgttctcgga tgtacaccgg
      721 cgagaggtgt cgctcgccgg aaccggcaaa caggaggagc cccgatgatc gagttcctgc
      781 tggcgaatgg caaccttcca ttcaccatcg ccctgaccgt catggtcggc atcgcgctgc
      841 tggaggcggc aggcaccctg ctgggcttcg gctccagcgc cgcccacccg gcgggcgccg
      901 acctggacgc cggcggcggc ccccatctcc acctggacgg ccacgccgac gtgcacgtgg
      961 accatccgga gctccatgca ggccaccatg gcgccctggc ccacgccgac catggcgatc
     1021 tgcatcacga cgtccatggg gacgcggaca gcgcctcggc gatcggcaag ctcctcgcct
     1081 ggctgggcct cggcaagctg ccggccatgg tctggctggt ggtcttcctg acctcgttcg
     1141 gcctcaccgg cctggcgctg cagatgctga tccgcggctt cgccggcggc ctgctgccgg
     1201 ctggggccgc cgcccccatg gccctgttcc tctgcctgcc cgtcgtcaag gtcggcggcg
     1261 ggctgctcgc gcggatcctc cccagagacg agaccagcgc cgtggcggcg gagtcgttcg
     1321 tcggccaggt cgcggtggtc acgctgggca ccgcccggcg cggccagccg gccgaggcca
     1381 agctcagaga tacttatggc aagacccact atgtcctggt agagccggag agctcgggcg
     1441 acgagctcga gaccggctcc caggttctac tcgtcagtcg cgcgggcgcg gtcttctccg
     1501 gcgtccgcaa cccgtacggg accctgatgg actgacccgc tccggcgagg tcagaaggag
     1561 ctctcaatgg aaaacctcat cttcatcgcc ttcgtcgccg ggatggcgct catcgccctg
     1621 ctggcgatcg gcttcatcct gacgcgcctc tatcaccgcg cctcgaagga ggagtccttc
     1681 gtccgcaccg gcttcggtgg ccagaaggtc atcctgaatg gcggtgccct gatcctgccg
     1741 gtcctgcacg agaccatccg ggtcaacatg aacaccctgc gcctggaggt gcggcggtcc
     1801 aacgagcagg cgctgatcac ccgcgaccgg atgcgggtcg acgtccaggc cgagttctac
     1861 gtgcgcgtca agcccaccga ggaggcgatc gccgacgccg cccagacgct cggcaagcgc
     1921 accatggacc cgaacgccct caaggagctg gtcgaggcga agttcgtcga cgccttgcgc
     1981 gccgtggcgg cggagatggc catgcaggag ctgcacgagc agcgggtcaa cttcgtccag
     2041 aaggtacaga ccgccgtctc cgaggacctg ctcaagaacg gcctcgagct ggagtcggtg
     2101 tcgctgactt cgctcgacca gacgaaccgt gagtacttca accccaacaa cgcgttcgac
     2161 gccgagggtc tgaccaagct cacggaagag atcgagacgc gccgcaagaa gcgcaacgac
     2221 atcgagcagg acacccaggt ggccgtgcag tccaagaacc tggaagccga gcgccagaag
     2281 ctcgagctgt ccaagcagga ggagtacgtg cgcctcgacc agcagaggga gatcgccatc
     2341 cggcgcgccg cccaggccgc cgagatcgcc cgcgagcagg ccgagaagaa ccgccaggcc
     2401 aaggaggccg agatcctcgc caagcagcag gtggaccttg cggacatctc cgcccaccag
     2461 gcgctggcgc agcagcggat cgagaccgag cgccaggtcc aggagctgga catcgtcaag
     2521 gtgcggacgc tcgagaccgc cgagatcgag aagcgcaagg ccgtggagat ggccgagcag
     2581 gatcgggcca tcgccatcgc cgagaagtcc aaggcacagt ccgaggccca ggctctggcc
     2641 gaccaggcgc gcgccgtggc cgccaaggcc gaggagcagg tggtgaccgc ccgcgaagcc
     2701 gagcgcgccg agcgggccaa gcagatcgag ctggtcaagg cgagccagga ggcggagcgc
     2761 gaggcgatcg gcatcaccac cggcgcgcgc gccgagaagc aggccgccga ggaccgggcg
     2821 accgccgtcc ggacgctcgc ccacgccgaa gccgaccagg ccaagatcgc ggcccagggc
     2881 gaggccgagg ccgaagtggt gcgtgccgaa gccgcggcca agcgctacga ggtcgaggcc
     2941 acgggtaagc gggccctgaa cgaagccgcc aatgccctga gccccgaggc gatcgcaatg
     3001 caggtccggc tcgccctgct cgagcacctg ccggagatca tccgcgaggc ggtcaagccc
     3061 atcgagcgca tcgaagggat caagatcgtc caggtgggcg gtctgggcct ggacggcgcc
     3121 gcaaatggcg acggccaggt ggttaccggc ggcggtggcg gtggcagcct cgccgaccag
     3181 gtggtcaaca gcgccctgcg ctaccgcgcc caggcgccgc tgctcgactc gctgctccag
     3241 gacgtcggcc tctccagcca cgagctcgcc ggcctgaccc aggccctcca cgccaagggc
     3301 aacggcggcg ccgagccggc gcacccgcag ctcccgcaag gggagacggt ggccgtccac
     3361 aagcccaagc agaagacgac ctgaccggcg tctgacagtc cgcggggcat ccggccgtcc
     3421 tcgaacggcg ggtgccccgc tacactcttc ccatgtccac cttcctgatc cgcgccgccc
     3481 ggcgaaggaa cctcatcccc cggatctcgg agaccgagcg ccaggccctg gaagccggca
     3541 cggtctgggt ggacggcgag ctgttctcgg gccggccgga tttccggcgc atgctgcgcg
     3601 agccctaccc catcctcgcg gacgaggagc gggccttcct cgatgggccg gtcgagcgcc
     3661 tctgcgccct ggtggacgac tgggaggtct cccgccggcg cgagctgccg ccgaaggtgt
     3721 gggacctgct caaggaggag cgcttcttcg gtctggcgat cccacgggag cacggcggcc
     3781 acggcttctc ccccctggcc ctctccacca tcttcggcac cctcgcctcc aggtcgctgg
     3841 cgctgtcggc cgtggtgctc atccccaact cggtggggcc cggcgagctg ttactggaat
     3901 acggcacgcc cgagcagcag gccttcttcc tcccccggct cgcccgtggc gaggagatcc
     3961 cctgcttcgc cctcaccgag cccgaggctg gctccgacgc cgcctctctc cgctccgagg
     4021 gggtcctctt ccgtgacagc aagggcgacc tagccatccg tctctcctgg agcaagcgct
     4081 acatcaccct cgctccggtc gccaccctgc tgggcctcgc cttccggctg cgcgacccgg
     4141 agaacctcct cggccgcggc gaggacgtgg ggatcacctt ggcgctggtg cccacccacc
     4201 tgccgggggt gcgcatcggc gaccgccacg accccatggg cgtccccttc cccaacggcc
     4261 ccacggaggg aagggacgtg gtgatccccg cggaccggat catcggcggg cccgagtacg
     4321 ccggccgcgg ctggaagatg ctcatggaag cgctgtcggc cgggcgctcc atctcgctgc
     4381 cggcgcagtc ggtgggcggg gccaagtacg tcgcccgggt ggcgggcgcc tatgccgcgg
     4441 tccgccgcca gttcggggtg gagatcggcc gtttcgaggg gatcgaggag cccctagccc
     4501 gcatcggggg tctcacctgg ctcatggagg cggcgcgggt cgccacctgc ggcgccgtgg
     4561 ggtcggggca ccggccggcg gtgatctccg gcgtggtcaa gtaccagcag accgagttgc
     4621 tccgccgcgt cgtccaggac ggcatggacg tcctgggagg cgccgccctc tctcgggggc
     4681 ctcgcaacct catggccgcc gcccacatgg gggccccgat cggcatcacc gtcgaggggg
     4741 cgaacatcct cacccggacc ctcatcgtct tcggtcaggg ggcgatccgc tgccatccct
     4801 atgcgctcgc cgagatccgc gccctggagt ccggggacgc ggcggcgctc cgcaaggcgc
     4861 tcctggggca cgcgggttcc ttcctgcgca acctcgtccg ctccgccctg ctgggcctca
     4921 cccgtgggcg cctggccgga tcgccggttg ccggccccac cgcgggaagc tggcgccgcc
     4981 tcgcctgggc ctcggcgcgg ttcgccctgc tgaccgacct cgccctcctg tccctgggga
     5041 gccggctgaa gttgcgcgag aagctcgccg gccgctttgc cgacgccctc tcctggatgt
     5101 acctgggctt ctgcgcgttg cggcgcttcg aggccgaggg acggcgcgag gaggacctgc
     5161 cgttcgtcct ctgggcgacc gagcacgccc tggaccagat ccagaccgcc ttcgagggga
     5221 tctaccgcaa cttcgagggt ccgctgggat ggctgatgcg cggtccgggc gcctggtggt
     5281 gccgggtgaa cccgctgggc cggccgccgt ccgaccgccg gggcgcacgg gtcgccgccg
     5341 ccctgcggag cgccggcgcg ctgcgggacc gcctgaccgc cggggtgttc ctccccgccg
     5401 atcccaccca ggcgctgggg aggctcgagc gggctttcgc gctggtggcc gaggcggcgc
     5461 cggtggccga gacgatcgcc cgcgcctccc gtcaggggac cctccccgcc ggccggccgg
     5521 aagagctgct ggactgggcc gtggccgccg gggtcgtcac cgcggagcag gccggactgg
     5581 tgaggcgggc cgccgaggcc cgcctcgatg ccattcaggt ggatagcttc gggctggagg
     5641 agtacctccg gctggagcgg cccaaagccg ctccagccga agccgtgcag ctcagttgag
     5701 cggacagccg aggttgttgt cgttgtccag cctggtggcg agggccagca tggtgtcgcg
     5761 gttgttgctg gccagggccg cgttgacatc ggagatgatg gccgaggcgg tccgggtgta
     5821 gtccacgccc gagtgcgcgg cgttgaggag cgccgcgacc gccgcccgca gcaggatctt
     5881 cgccgcaccc agcgagcccg ggccgccgcc accctggagg gcctgcagca gggtctggct
     5941 ccccagcgac gggaagccgc cggccgagaa gacgctgcct accgtctggc ccggcgtgta
     6001 gccggtgccg gcccacgact cggtgtgatt cttccagtag ccgggcgtgc agccctcgtc
     6061 accggtgacc ggcggcggag gaggaggcgt caccaccggc agcgggatgc gcagggcgcc
     6121 caggatccag agcagcgagt ccgggttctg gttcaacggc tcggagaaga gctgcaccgt
     6181 ggtcgaggag acgcccgccg gtacgttgac ggtgatggcg tccgtgtccc acgacgcgcc
     6241 gtccgaagca ttcaggccgt tgaccacgct ggggttgttg ctgatgtcga tacggtccgg
     6301 acggttcggg gtgccgtcgc ccgagaacac ccacagcgtc gccgaccggg ccgacgtgga
     6361 cgaaccgtgc gagaaggtca ccggcaccgt cacccgggtg gggccggggg tcggatcgat
     6421 cccataggcg aagtccagac cgtcatagac gatcaggcga tacatggcgg tgtcggccgg
     6481 gttcgtgtag atcaccacca ggcccgcgcc gttcaggtgg aagaggttgc tggccgtgtt
     6541 gccgtcctgg atcgggaagg tctgcgagcc cgtgccccgc gcctgcacga tcgaggtgac
     6601 atccgccagg aagccgatat tgttgatcgg gccgcccgcc gaggccggct gtgtctccgt
     6661 gccaatgatc gtgccggtga tcaggctgcc gtcgaaacgc agcacctgat ccttgtatgg
     6721 ctggctgggg atgatgcagc tcccgccgct catcgggcag ggacggtccc gccccgtcca
     6781 gtagagcagc gctgcctgga ccgttcccgg gatgtttacc gtcaggttcc tcgttccgct
     6841 tccaatcgcc tgcagtccca cgcccccttc ggtcatcgcc agaccttgcc cctggatatc
     6901 cagcgccacg ctcagcgtcg tcgcctggac tgttccgcca agaagcacca tgcacagaac
     6961 ggccagacac agctttacaa cagacactct catccttaca cctccgtgaa gaaaaaacat
     7021 ctgtgggacc tctcgaggga ggggctgccg cgatgcgcgg cgcaaagagc tacgagtgtt
     7081 gccccagatc gagtgcatcg tgcgactctc cccgggcttt tcgggagccg ggcgatctcg
     7141 gatgagatcc ctggctttgc ggccccgcct cgcgacgggt gtgccttttc ggagcctcag
     7201 agcaaagatg gttcagtaga agatcagcct ccgcacagtc caaggaattg tgcagagtcc
     7261 gtaccatcgc tctttggcta ttcggtgatg ttcgatagag ggaattggta caatgagctg
     7321 cccgctttcg aacccttggg aggatcctga atgcccctgt tccgagtgct cgccgctctc
     7381 ctgcttctgc cggtctgcgt cgcggtcccc gtggccgccg ccaccggcag ctcgaccgac
     7441 cccgtctacc gcacgccccc tcaggcgctg gtggatatcg tcgacgcgcc tccgactccg
     7501 gacgtggacg tggatccgag acgggagtgg ctgctgctcc tggagcgccc caacctgcca
     7561 ccgatctcgg agctggccga gcgcgagctg cgcctggccg ggctgcgcat ccggccgcgg
     7621 acgagcggac cgagccgcac ccgctacttc accggcctgg agctgctccg cgtcgcggac
     7681 ggctcccggc gccggatcac cggcctcccc accggcgcca ggatcgaaaa catccgctgg
     7741 tcgccggacg gctcacggct cgccttcacc cacaccgggc aggaagccat cgagctctgg
     7801 gtggcggagg tggccaccgg ccaggcccga cggctggggg atgcccggct caacctcgtg
     7861 gcgcgtaccg tccccgtctg gctgcgtgac ggccgcggcc tcgtggccac cctggtcccg
     7921 gcggaccgcg gggccgagcc ctcggtgtcc caggtgccca cggggccggt gatccaggag
     7981 agcctgggcc gcacggcgcc cgcccgcacc taccaggacc tcctcaagag cgcctccgac
     8041 gaggccctct tcgagcacta tctcacctcc cagctcgccc ggatcgacct cgatggccgg
     8101 gtcacgccgc tcggcgcccc cggtctcctg gaccgcttct cgacctcgcc cgacggccgc
     8161 tacctgctga ccgagaccct gcaccgcccg ttctcctacc tggtgccggc gagccgcttc
     8221 cctcggcgga tcgaggtttg ggacctcgcc acgggccagc gggtgcggga gatcgccgac
     8281 gtgccgctcc aggacgaggt ctcgacccag ttcgacgccg tccccaaggg gccgcgccag
     8341 ctcgagtggc gggccgatgc ccccgcgacc ctggtctggg ccgaggcgct ggacggtggc
     8401 aacccctccg ccccggccga gcaccgcgac cgcatcgtgg ctctggcggc gcctttcgtg
     8461 ggggatcccg tgatcctcgc caccctgccg ctccgctact cgggcgtctc ctggggaaac
     8521 ggtgacaccg ccctggtgcg cgatcggtgg tggaaagacc gccggatccg cacctggcga
     8581 atccggcccg actcgcccac gtccgctccc gatctcctct tcgaccgctc ctccgaggac
     8641 cgctacagcg atcccggcgt tccggtaatg gtcgagaacg aggccggccg ccacgtgatg
     8701 ctgctcgccg gcggcggcaa gacgctctat ctgaccggcg acggcgcctc gcccgagggg
     8761 gaccgcccct tcctcgacgc ctacgacctg accaccagga aggcacggcg actcttccgc
     8821 tcggaggccc cgttctacga ggatccgctc ctgccgttgc cgtcgcggga taacgcggta
     8881 cgcgagctgc tggtccgccg cgaatcggtc atggagccgc cgaactactt cgtccgtgac
     8941 ctgcggaccg gcaagctccg ccagctcacc tccttccccc atccgatgcc gcagctcgcc
     9001 ggcctccaga aggagctcgt ccgctacaag cgggacgacg gcgtccagct cacggcgacc
     9061 ctctacctgc cgcccggcta caagccggcc gacggcccgc tgccgctgct gatgtgggcc
     9121 taccccgagg agttcaagag cgccgacgcg gccagccagg tccaggactc cccctaccgc
     9181 ttcgaccggg tggcgccggg ctcgccgctc gtctggctcg ccgccggcta cgccgtgctc
     9241 gacaacccca ccatgccgat cgtcggcgcc ggcgacgcgg agccgaacga cacctacgtc
     9301 gagcagctcg tggcgagcgc cagggcggcg gtggacgaga tggtgcgccg cggcgtggcg
     9361 gacccgcgcc gcatcgcgat cagcggccac tcctacggcg ccttcatgac cgcgaacctc
     9421 ctcgcccact ccgacctctt cgccgccggc atcgcccgca gcggcgccta caaccgcacc
     9481 ctcaccccct tcggcttcca ggccgaggag cggttgctgt ggaaggcctc cgagacctac
     9541 ctgcggatgt cccccttcat gtacgccgac aagatcaacg agccgatcct cctgatccac
     9601 ggccaggtgg acaacaactc cggtaccgac cccatccaga gcgagcggtt ctacagcgcg
     9661 ctcaaggggc agggcgctac cacccgtctg gtgatgcttc ccggcgagag ccacggctac
     9721 gccgcccgcg agtcgctcct ccacatgctc tgggagactc actcgtggct cgagcggtac
     9781 gtgaaaaacc ggccggcgga ggttgtctcg tcctcagcct cgaaagcacc tcgttgatat
     9841 agggaatgtt ccctaactca aaggagtccc gtatgaccta cgaagcgccc gaggttttcg
     9901 agctgggcag tgccgaagag ctgacccttg gctcttgtgg cggtccgatg gactggtgcg
     9961 gcttctacat catcgatatc cagggctgtg agccgccctg tggcgtccac gaggagccga
    10021 gggaagagta gggttatcga ggctcccggc aaggtccacc gcgccgggag ccctccctcc
    10081 cttcatcatg gatggacgat tcgaagccgc ggagcacgtc cgctccagca ccggcgagga
    10141 ggggaccgtc ctcctcgacc tgcggtcggg caagtacttc gccctcaacg cggtgggcgg
    10201 gctcctctgg gagtcgatgg cggcgggggc ttcccggagc gtcctcgtgc agcggctcgc
    10261 cgagcgtttc cccgaggtcc cggccgaccg gctcgggcac gacgccgacg ccctgctctc
    10321 ccagctcctg gccaaaggtc tgctgcggcc caggaccggc cccggggatg aaaaggaggc
    10381 gcccgcgccc cgccctgttt ttcagcccgt gtggaaggac ggctcggctc tcgaggcagc
    10441 caccgcctcc ccgttctggg tccccatagc cttcctcgcg ctgctggcct cggacttcat
    10501 cctccaggtg ctgggcttct cccgcttcca cggtctggtg cggcgtatcc ccacccgtcc
    10561 cgcggcggct tcggcggcgc gggcgcagcg catcgtccgc agcgtcgacc gggcctctgc
    10621 cttctatttc aagcgtgcct ggtgcctgca gcgctccgcc gtcaccgcgg gcctgctccg
    10681 cctctcgggc ctccccgccc ggctggtcat cggcgcccgg cgggtgccct tctacgccca
    10741 cgcctgggta gagctcgacg gccgcgtggt caacgaccgt cccgccgtgc gccaggagta
    10801 cgaggtcctg gagacctgct gagcgccccg ccggcgatcc caccatgagc gccttcggcg
    10861 gcctctactc tccgggtgcc ccccctccgg acaccgggat cctctcggcc ctcacctgtg
    10921 ccctcgggcg gcgcggcccg gacggcgttt ccgtggcctc catgccggag ggcgcgatgg
    10981 ctcacgcctc tttccacacc gcgccgggtc cacccgcgga gcagccctgc cggcacggag
    11041 cggtcctcgt cacctgggac ggccggctgg acaatcgcgc ggacctcgcg gcgtccctcg
    11101 gcgcggagcg cgcgaacgac gtggagctgc tggctctcgc ctacgagcgc tggggcccga
    11161 ccttcccttc ccggatcgtg ggagacttcg ccctcgccct gtgggatggc cgcgcccggc
    11221 tcctcctcct ggcgcgggac ccggtgggga cacgccccct cttctatgcc gtccggccgg
    11281 acggcgggct tctctgggcc tccacgctgg cggccctccg cgccggcggg gccgacctcg
    11341 gcgaggtcga cgaggagtgg atcgcgggct acttctcgtg gtccatcgat ccggccacga
    11401 cgccctggcg cggcgtgcgg gcggtgccgc ccgggcacgt cctggccgtc ggcggcgggc
    11461 cgccccggct ccagtgcttc tggaaggccg aggaggtccc cgcggtccgc ctcggcggcg
    11521 acgccgaata cgaggagcgc taccgcgagc tgttcctggc ctccgtccaa aaccgtctac
    11581 gcgccgcggg accggtggcg gccgagctct ccggcggcgt cgactcctcc tccatcgtct
    11641 gcgccgcggc ccacctgctg cgcgccggca gcgccgagac cccgggcctg ctcaccgcgt
    11701 cccacctcta tgagcgctcc cggacggccg acgagacccc cttcatcgaa gaggtggagg
    11761 cgttcatcgg ccgcaccggc cggcacatcc tggagagcga ggcgccgatc ctcaccgggc
    11821 tcgtcacgga catcccggag atcccgacct ccaatcactg cttccggcgg cgctatcagg
    11881 ccatctacga cttcatggcg gagaacggct cccgcgtact gctctccggc ctgggcggcg
    11941 accacctgct caccagccag gtagccactc cgtacgtcct ggccgacctg ctccgcggcg
    12001 ggaggctcgg agctctccgc accgggctcg ccgcgtggcg gcgggagctc ggcaagccgt
    12061 accttcagct cctctgggag ggagcggtct gtccgctgct gccccggccg gcccggatgc
    12121 gcctcgcccg ccctccgatc ccctttccct cctggctcga tcgtggcttc gtccgccgcc
    12181 gggacctcgc cgcccgcttc gtcgccctga ccgacgccga ggggtacccc ggccctcccg
    12241 gcaaacggga gcaagccggg gccgtgcgag gggcgctgcg cgccgcggcc tggctgtacg
    12301 actggtgggg gcggccggtg gaggtgactt ttccctttct cgaccgcccg ctgatggaat
    12361 tctgcctcgg cgcccctgcc ggacagttcg tccgccccgg ggagacccgc tcgctgcacc
    12421 gccgggccct cgcc
//
LOCUS       DASPJI010000149         3038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_122664, whole genome shotgun sequence.
ACCESSION   DASPJI010000149 DASPJI010000000
VERSION     DASPJI010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122664"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VEL74_03985"
     CDS             <1..623
                     /locus_tag="VEL74_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61718.1"
                     /translation="GEDLLFGILLSLCFGEARVGHLSWVAAHEPMEVRRFWPGEMQRS
                     ASGIDHSRLVSALLDGFTPDPEADPARNLRALGEHLTSLGSLAPADFDERGRLAVLGR
                     AEAFAEELERRLEAWRDEPEAETPGGALWAEDVRNYLALLRQHAARPDFAVPLDLLYG
                     REPEEARRLAQHLLSDYGRLLTVWPDLVAAARELRAQGVEMAEEVR"
     gene            620..2176
                     /locus_tag="VEL74_03990"
     CDS             620..2176
                     /locus_tag="VEL74_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG61719.1"
                     /translation="MTERTALLGRVDALSPGRRALFELLLLEEAAGGRGTREDTGRAP
                     RATASAPLSFGQERMWLHERLQPGGGAYNTSMALRLLGPLDPGTLRRSLEEVVRRHET
                     LRTTFSEERGSPVQTAGPPFSVALPMADVAALPAEAREAEARRLAGRELLRPFDLWRG
                     PLFRVLLLRLEREEHVLSVSMHHIVSDAWSMGVLVRELSVLYQAFAAGRPSPLPELPM
                     QYAELVRWQRGRLEGEGLERLLNWWRERLAGAPEVVEVPTDLPRPAVRCFRGGLRVRQ
                     LPPALAAAAARLGGEQSATLFIVLLAAFELLLHARTGRSDLVVGVDVANRTRPEAEAI
                     IGLFVNQLVLRVGLQGDPPFLELLERVRETAAGAYAHQDLPFGHLVEELAPERSLSRN
                     PLFQVMFGLYNVPAPAAEEGGLEVRPLELEAGAAVFDLSLYVQESAEGLACRLGYDSD
                     LYTPATAERLLEDYELLLRWALEAPEARVSEILARLEAEKRRRQAEEGGELRKSRAAV
                     LKTARRRTAE"
     gene            2274..>3038
                     /locus_tag="VEL74_03995"
     CDS             2274..>3038
                     /locus_tag="VEL74_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYG61720.1"
                     /translation="MEQQVHRFRNNAKAIAIGNPGWEQAREHNLIDLRVRVAGNDRLE
                     VEGTGFQFVNLSSCSYLGLHDYPAILEGAIEALRSAKTMDVPITRVRIRLNLVDELEA
                     RLSDLFRAPCISSVTASAATTGVLPLIASGHLSGGQPPLMIFDKFCHFSMNLIKPICA
                     DETQVVTAPHNDLNFVEDACKKHPQVAYVADGAYSMGGAAPVRELMELQDRYGLFIFL
                     DDSHSLSVYGERGVGYARSLMGDEMPPRTIIVGSLCK"
BASE COUNT          413 a         1091 c         1049 g          485 t
ORIGIN      
        1 ggggcgagga cctcctcttc ggcatcctcc tgtccctctg cttcggcgaa gcccgggtgg
       61 gccacctctc ctgggtggcg gcgcacgagc ccatggaggt ccggcgcttc tggccgggcg
      121 agatgcagcg tagcgcctcc ggcatcgacc actcgcgcct ggtctcggct ctgctggacg
      181 gcttcacccc cgatcctgaa gccgatccag cccgcaacct ccgcgccctt ggggagcacc
      241 tgacctccct ggggagcctc gcccctgctg acttcgacga gcgggggagg ctggcggtgc
      301 tgggccgggc cgaggcattc gccgaggagc tggagcggcg gctcgaggct tggcgggacg
      361 agcccgaggc ggagaccccc ggcggcgcgt tatgggccga ggacgtgcgc aactacctcg
      421 ccctgctgcg gcaacacgcc gcgcggcccg acttcgcggt tcccctggac ctgctctatg
      481 gccgggagcc cgaggaggca cggcggctcg cccagcatct gctatcggat tacggcaggc
      541 tgctcaccgt ctggcccgac ctggtggcgg cggcgcgaga gctccgcgcc cagggggtcg
      601 agatggcgga ggaggtgcga tgaccgagcg gaccgcgctg ctgggccgcg tcgacgccct
      661 ctcaccaggg cgccgcgccc tgttcgagct gctgcttctc gaggaggccg ccggggggag
      721 ggggacccgg gaggacacgg ggagggcacc ccgcgcgacg gcctccgccc ccctctcgtt
      781 cgggcaggag cggatgtggc tccacgagcg tctccagccg ggagggggcg cctacaacac
      841 ctccatggcc ctccgcctcc tgggccccct ggaccccggg acactgcgcc ggagcctcga
      901 ggaggtggtc cgccgccacg agaccctgcg caccaccttc tccgaagaga gaggctcgcc
      961 ggtccagacc gccggtccgc cgttctccgt ggcgctcccc atggccgacg tcgccgcgct
     1021 gcctgccgag gcacgggagg ccgaggcccg gcggctggcc gggcgggagc tcctccgccc
     1081 cttcgacctg tggcgggggc cgctcttccg cgtcctgctc ctccggctgg agcgcgaaga
     1141 gcacgtcctg tccgtgtcca tgcaccacat cgtctcggac gcctggtcca tgggcgtcct
     1201 ggtgcgggag ctgtcggtcc tttatcaggc gttcgccgcg ggacggccct cgcccctgcc
     1261 ggagctgccc atgcagtacg cggagcttgt ccgctggcag cgcggccggc tcgaaggaga
     1321 ggggctggag cggctcttga attggtggcg cgagcggttg gccggagctc ccgaggtggt
     1381 ggaggtccct accgacttac cccgtccggc cgtccggtgc ttccgcggcg gcctgcgggt
     1441 gcggcagctc ccgccggcgc ttgccgcggc ggcggcgcgg ctgggtggag agcagtcggc
     1501 gaccctgttc atcgtgctgc ttgccgcctt cgagctgctc ctccacgccc gcaccggccg
     1561 ctccgacctg gtggtggggg tcgacgtcgc caaccgcacc cgtccggagg ccgaggcgat
     1621 catcggcctc ttcgtcaacc agctcgtgct gcgcgtcggc ctccaggggg acccgccctt
     1681 cctggagctg ctggagcgcg tgcgggagac cgcggcgggc gcctatgccc accaggacct
     1741 ccccttcggc cacctggtgg aggagctggc gccggagcgc agcctgtccc gcaacccgct
     1801 cttccaggtg atgttcggac tttacaacgt ccccgccccg gcggcggagg agggggggct
     1861 ggaggtccgg cccctggagc tggaggccgg tgccgcggtc ttcgacctga gcctgtacgt
     1921 ccaggagagc gccgaagggc tcgcctgccg gcttggctat gactccgatc tctatacacc
     1981 cgctaccgcc gagcgtctgc tggaagacta cgaattgctg ctgcgctggg ccctcgaggc
     2041 gccggaggcc cgcgtgtccg aaatcctcgc ccgcctggaa gccgagaagc ggcgccggca
     2101 ggcggaggag gggggcgagc tgcgcaagag ccgcgccgcc gttctcaaga ccgcccgcag
     2161 gcggaccgcg gagtagccgt acacgatctc atcgccatct gttccctccc gggtgtctgg
     2221 agacaccttt tattaggatg gctgtccgcc gctcttctac aggagaccac cacatggagc
     2281 agcaggttca tagattccgc aataacgcca aagccatcgc gattggcaat cctggctggg
     2341 agcaggcgcg cgagcacaac ctcatcgacc tgcgggtgcg cgtcgccggc aacgaccgcc
     2401 tcgaggtgga ggggaccggc ttccagttcg tcaatctgtc gtcctgttcc tacctggggc
     2461 tccacgacta tccggccatc ctcgaagggg ccatcgaggc gctgcggagc gcgaagacga
     2521 tggacgtgcc catcaccagg gttcgcatcc ggctcaatct ggtggacgag ctcgaggcaa
     2581 ggctctcgga cctgttccgc gcgccatgca tctccagcgt cacggccagc gccgccacca
     2641 ccggcgtgtt gcccctcatc gcctcgggcc acctctcggg cggccagcct cctctcatga
     2701 tcttcgacaa gttctgtcac ttctcgatga acctgatcaa gcccatctgc gccgacgaga
     2761 cgcaggtggt gaccgctccc cacaacgacc tcaacttcgt cgaggacgcc tgcaagaagc
     2821 acccgcaggt ggcctacgtg gccgacggtg cctattccat gggcggtgcc gcgccggtgc
     2881 gggagctgat ggagctgcaa gaccgctacg gactgttcat cttcctggac gactcccatt
     2941 cgctgtcggt ctacggcgag cgcggcgtcg gctacgcgcg ctccctgatg ggcgacgaga
     3001 tgcctccccg aaccatcatc gtaggctccc tctgcaag
//
LOCUS       DASPJI010000150         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_694462, whole genome shotgun sequence.
ACCESSION   DASPJI010000150 DASPJI010000000
VERSION     DASPJI010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694462"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /gene="ribD"
                     /locus_tag="VEL74_04000"
     CDS             <1..1120
                     /gene="ribD"
                     /locus_tag="VEL74_04000"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009655946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HYG61721.1"
                     /translation="SDEARRLGQALRLAMRGRYGTSPNPMVGALLVRDGQLLGSGWHR
                     QVGGPHAEVEALRDAEKRKRDVRGATLYVTLEPCSHHGRTPPCTDAVIAAGIARVVAC
                     HVDPDPRVAGRGYAQLRAAGIEVEVGILVEEAVRLNLRFLVEKILHRPAVTLKWAMSL
                     DGRIATATGQSQWISSPQGRRWALALREEHDAILVGSGTVLADDPRLDRRLDLAGRPN
                     LRVVMDRRLRTEPGAKLFTVEGPVLLYTETDDARRRRALEKKGAEVIVLPAVEPSAVL
                     EDLYERDVQSVLVEGGGEVLASFTASGCWDQVGVDCAPLLIGGRGAPGPLGGEGFATL
                     DHAARLEHLDFRRLGGDLVFTAYKKGRIGELVKRVSGL"
     gene            1533..1886
                     /locus_tag="VEL74_04005"
     CDS             1533..1886
                     /locus_tag="VEL74_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61722.1"
                     /translation="MHDHHHGTPSGDRTPTPFESLDNTEATRRALYDAVVRVHFQPSP
                     QEIQRAQEDPENYWIPTYTPDQAGLAILWIFGRWFVVWRALEETEGPETLKWTVLRIV
                     PDPEASPVPLQFLEV"
BASE COUNT          372 a          857 c          801 g          349 t
ORIGIN      
        1 gagtgacgag gcgcgccggc tggggcaggc cctccggctc gccatgcggg gccgctacgg
       61 cacctcgccc aaccccatgg tgggcgccct cctggtgcgc gacggccagc tcctgggcag
      121 cggctggcac cggcaggtcg gcggtccgca tgccgaggtc gaggccctcc gcgacgctga
      181 gaaacggaag cgcgacgtcc gcggcgccac cctttacgtt accctggagc cctgctccca
      241 tcacgggcgc accccgccct gcaccgacgc ggtgatcgcc gccggcatcg cccgcgtcgt
      301 cgcctgtcac gtcgaccccg acccgcgggt ggccggccgc ggctacgccc agctccgcgc
      361 cgccggcatc gaggtcgagg tgggaatcct ggtggaggag gcggtgcgcc tgaacctccg
      421 cttcctggtc gagaagatcc tgcaccgccc cgcggttacc ctgaaatggg cgatgagcct
      481 ggacggccgc atcgccacgg ccaccgggca gagccagtgg atctcctccc cgcaaggccg
      541 ccgctgggcg ctcgccctcc gcgaggagca cgacgccatc ctcgtcggca gcggcaccgt
      601 gctcgccgac gacccccgcc tcgaccgccg cctggatctc gccggccgtc ccaacctccg
      661 cgtggtcatg gaccgccgtc tccgcaccga gccgggggcg aagctcttca ccgtcgaggg
      721 ccccgtcctc ctctatactg agacggacga tgcccgccgc cgccgcgccc tggagaagaa
      781 gggggccgag gtgatcgtcc tgccggccgt cgagccctcc gcggtcctcg aagacctcta
      841 tgaaagggac gtgcagagcg ttctggtgga agggggaggg gaggtcctgg cgagcttcac
      901 cgcgagcggc tgctgggatc aggtcggagt cgactgcgcc cccctcctca tcggcggccg
      961 gggagctccc ggcccgctgg gaggcgaagg cttcgcgacg ctcgaccacg ccgcccgcct
     1021 ggagcacctc gacttccggc gcctgggggg cgacctggtc ttcaccgcct acaagaaagg
     1081 gcggatcggg gagctggtga agagggtgtc ggggctgtaa cgcaccagca tcgcgggggc
     1141 ggtggtgcct cccaccctgg gatggtgggc gggagggagt gggggaggtc aggagggggg
     1201 cagcttttcg ccccggctcg gcgcggactc ccccagccct ccaaaacaac cccccacctc
     1261 ggtcctcccc cgctccctcc cgcccaaccg cccagggggg gaggaagcca ccgcccgggc
     1321 gtgacgagct gaagtctctc cagccccctt gacgccccga tttcactgag ataaagctgc
     1381 ccgcagtcgt tccgccgtac cgctgttctg ttctttccag gctgttcccg ggcgaggaat
     1441 acgtcttcgt ccggattgaa cccgcggcga gcggccccaa gtggaggtga tgactccacc
     1501 cagggcctag ccgcacaagg agacttcact ccatgcacga ccatcaccat ggtaccccgt
     1561 ccggggaccg cacccccacg cccttcgagt ccctcgacaa caccgaagct acgcgccgcg
     1621 cgctgtacga cgccgtggtg cgggtgcact tccagcccag cccgcaggag atccagcggg
     1681 cgcaggagga tccggagaac tactggatcc ccacctacac gccggatcag gccgggctcg
     1741 ccatcctctg gatcttcggc cgctggttcg tcgtctggcg agcgctggag gagacggagg
     1801 gacccgagac gctgaagtgg acggtgctgc ggatcgtccc cgaccccgag gcgagccccg
     1861 ttcctctgca gttcctcgaa gtctgacgcc gtacccccgg cggggagccg tcgctcgacg
     1921 ctctccgccg gttcttcacg gccatccgcc gtcattcatc cgactacgac ccgcgggaga
     1981 cgaagaatac aggcaggtac aggcctgtac cggaatgggg aggaggctct cccattttgg
     2041 ggagggggtg tacctaaaag gtgaaggggt agtaccggat gtgggaggga gccgtaccct
     2101 ttacgggaag agcgcctccc ggattaggga ggaggtctcc cttttcaggg aatccccttt
     2161 cccgaaaggg gaggacggca cacccttttg gggatcgcgc cctccacaag gggatggagg
     2221 cctcacccct gccgtccgag ggatcttcca ccccgaaggc ggcttctccc cgaggcagga
     2281 taccagaggc cgcccgccgc catccggcgg gcggactaca gattctgcta ctagcaaccg
     2341 tccatgatca tgacgtccgg gcagcccggg ggaggcggc
//
LOCUS       DASPJI010000151         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_811375, whole genome shotgun sequence.
ACCESSION   DASPJI010000151 DASPJI010000000
VERSION     DASPJI010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811375"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VEL74_04010"
     CDS             complement(<1..1281)
                     /locus_tag="VEL74_04010"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain-containing protein"
                     /protein_id="HYG61723.1"
                     /translation="MDATYSPQSRAERLIATGRVVLAASSLFAVWLDPSEPAKYAGTA
                     YALLAAYVAYAVVIAVQVWRSDTPSNRHRLVTHAFDLCFFSLFIYFTSGTASPFTAYF
                     VFSLVCATLRWQWQGTLWTALVSLAAFLAVGIYFSQVVEDPAFEPYPLVVRGVYLAVV
                     AVLLGYLGAHEERTRRDMTMLATWPAAAPQRVETLVRALLEHAVLAMGSPRVLLVWTE
                     REEPWHYLGVWNQGVFTSSRMSPGTLGQLVAEPLRDAAFLCPDVGRPAPTVLRGPGGA
                     LQRWPGPPLDPELLRRFIVHAVLSLPFRGESAEGRLFFLEKPRMTSDDLVLGEIVAGV
                     VCSRLDHFYLTQRLQETVATEERIRLARDLHDGVLQSLTGVGLRLAAVRGLLEEDPRA
                     AREGMEALQRLIALEQRDLRFFIQELKPSAQGPAG"
     gene            1439..2008
                     /gene="pal"
                     /locus_tag="VEL74_04015"
     CDS             1439..2008
                     /gene="pal"
                     /locus_tag="VEL74_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HYG61724.1"
                     /translation="MRSRLFVCLILSTLLAAPGCRPKKPPATQADLPVETITAAPEAP
                     APAPGPAAPEPGPFDPLAGDLASVNEYLRQQGLLQDVYFDYDQATLKQEVRDQLARSA
                     RFLNDNPRFSVTLEGHCDERGTNEYNLALGERRATTVRDYLVSLGVDGSRLRTMSYGE
                     ERPACGDPDESCWRLNRRAHPVVTGRSGG"
     gene            2306..>2678
                     /locus_tag="VEL74_04020"
     CDS             2306..>2678
                     /locus_tag="VEL74_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HYG61725.1"
                     /translation="MKIATWNVNSIRARLARVLAWTERELPDVLCMQEIKVPEDDFPL
                     APFAGLGYEAAIYGQRTYNGVAILSRLPLTDVERGLPGEGPDDHRRLIAATVAGIRVI
                     DVYVPNGQSPESDKFVYKLEWL"
BASE COUNT          436 a          963 c          926 g          353 t
ORIGIN      
        1 ccccgccggc ccctgagccg acggcttgag ctcctggatg aagaagcgca ggtcgcgctg
       61 ctccaaggcg atcagacgct gcagggcctc catcccctcc cgcgccgccc gcgggtcctc
      121 ctccagcagg ccgcgcaccg ccgcgaggcg caggccgact ccggtcagcg actggaggac
      181 gccgtcgtgc aggtcgcggg cgaggcggat gcgctcctcg gtggccacgg tctcctgcag
      241 ccgctgggtc agatagaagt ggtccaggcg tgagcagacc acgccggcca cgatctcgcc
      301 cagcaccagg tcgtccgacg tcatccgcgg cttctccagg aagaagagcc gcccctccgc
      361 cgactcgccg cggaacggca gcgacagcac ggcgtggacg atgaagcgcc gcagcagctc
      421 cggatcgaga ggcggccccg gccagcgctg gagggcgccg ccggggccgc gcagcaccgt
      481 gggagccgga cggcctacgt cgggacagag gaaggcggcg tcgcgcaacg gctccgccac
      541 gagctggccc agcgtccccg gcgacatccg gctcgaggtg aagaccccct ggttccacac
      601 ccccaggtag tgccagggct cctcgcgctc cgtccacacc agcagcaccc gcggcgatcc
      661 catggccagc accgcatgct ccaggagggc gcggacgagg gtctcgacgc gttgcggcgc
      721 cgccgcgggc caggtggcga gcatcgtcat atcccgccgg gtccgctcct cgtgcgcgcc
      781 gagatagccg aggagcaccg ccacgaccgc caggtagacg ccgcggacta ccagcgggta
      841 gggctcgaac gcgggatcct ccaccacctg gctgaaatag atgcccaccg ccaggaaggc
      901 ggcgagcgag acgagggcgg tccacagcgt cccctgccac tgccagcgca acgtggcgca
      961 caccagggag aagacgaagt aggcggtgaa cgggctcgcc gtgcccgagg tgaagtagat
     1021 gaagagcgag aagaagcaga ggtcgaaggc gtgggtgacc aggcggtggc ggttcgacgg
     1081 ggtgtcggag cgccagacct ggacggcgat gaccaccgcg taggcgacgt aggcggccag
     1141 cagcgcgtag gccgtcccgg cgtacttcgc gggctccgac gggtcgagcc agacggcgaa
     1201 cagcgacgac gccgcgagga ccacgcggcc cgtggcgatc aggcgctcgg cgcggctctg
     1261 cggtgagtag gtggcgtcca tccggtccga tgatagcggc ccggccgggg gctgcgaatc
     1321 tatccaaagg tagaaacgaa ttctatccgc cggcgaattc acaggcccgc ccctccctcc
     1381 catactgccg ttgccgcggg cctgatgccc gccccgctga aagctgacag gagattgcat
     1441 gcgttccagg ctgttcgtct gtctgatcct ctcgaccctg ctggcggccc ccggctgccg
     1501 gccgaagaag ccgcccgcca cccaggcgga cctgccggtc gagaccatca cggccgcgcc
     1561 cgaggcgccc gctccggctc ccgggcctgc ggcaccggag cccgggccgt tcgatcccct
     1621 ggcgggcgac ctggcctcgg tcaacgagta cctgcggcag caggggctgc tgcaggacgt
     1681 ctatttcgac tacgaccagg ccaccctcaa gcaggaggtg cgcgaccagc tcgcgcggag
     1741 cgcccgcttc ctcaacgaca acccccggtt ctccgtcacg ctcgaggggc actgcgacga
     1801 gcggggcacc aacgagtaca acctggccct gggcgagcgc cgcgctacca cggtacggga
     1861 ctacctggtg tcgctgggcg tcgacggcag ccgcctccgc accatgagct atggggagga
     1921 gaggccggcc tgcggcgacc cggacgagag ctgctggcgg ctcaaccggc gcgcccaccc
     1981 cgtggtcacc ggccgttcgg gcggctgagg ggctcccaca ttcagaagag tttgtcggcg
     2041 cccgcttcac tcccggcggg cggtgacggg cggcctctcc gggccgccct tttttctttc
     2101 ccggcttccc tctcgaacca caaaaaagac cccgcgctct ggcgcggggt ggaggaggga
     2161 ggttggagag gagattgtcc tgtctgccgt ctcctactta tttatacgga tgagaccggc
     2221 aaagtgttca ggtcctcccg gcgcgagcca gcgctcgagc ccgcgcttga gcccggaagg
     2281 cggcccctga catgatctcc aggccatgaa gatcgccacc tggaacgtga actcgatccg
     2341 cgcccgcctg gcccgggtcc tcgcctggac cgagcgcgag ctcccggacg tcctctgcat
     2401 gcaggagatc aaggtccccg aggacgactt ccccctggcc cccttcgccg gcctcggcta
     2461 cgaggccgcg atctacggcc agcgcaccta caacggggtg gcgatcctct cccgcctgcc
     2521 gctcacggac gtcgagcgcg gcctgcccgg cgaggggccg gacgatcacc gccggctgat
     2581 cgcggcgacg gtcgccggca tccgggtgat cgacgtctac gtgccgaacg ggcagtcgcc
     2641 ggagagcgac aagttcgtct acaagctgga gtggctcg
//
LOCUS       DASPJI010000152         3965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_240625, whole genome shotgun sequence.
ACCESSION   DASPJI010000152 DASPJI010000000
VERSION     DASPJI010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240625"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VEL74_04025"
     CDS             <1..544
                     /locus_tag="VEL74_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61726.1"
                     /translation="LNEIEQKEKFDRLFLVGSQETMTEIENALPAPLAAKLAGKQNVD
                     FSAGDDQIFDASFDMHFAAEREAESQLWEKIKGEYCRGGLAVTGADEVLKAAAVGRVE
                     KMAVTRDARISGTRCRECENLSNGELSKCPICSSTEVFKVDLVNELVELLALSSADTD
                     FVDPIEGLSQAGDVAALLRY"
     gene            complement(592..1161)
                     /locus_tag="VEL74_04030"
     CDS             complement(592..1161)
                     /locus_tag="VEL74_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61727.1"
                     /translation="MRIQRPWRALFPLVLLLAPLRAGGAEAPAPASPAQAFFQNLAAH
                     CGKSYEGKVLFTTSKDFEGQRLVMHVASCSEKEIRIPFQVGEVRSRTWVLTLEGDRLR
                     LKHDHRHEDGTPEEVTNYGGDSDDTGTALRQTFPTDEFTRKLLPHAGGNAWTLEIDPA
                     AQEFRYSLDRGDEKRFRSSFDLSKPLPAS"
     gene            complement(1158..2117)
                     /locus_tag="VEL74_04035"
     CDS             complement(1158..2117)
                     /locus_tag="VEL74_04035"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HYG61728.1"
                     /translation="MDNLCHTLVGAALGKAGLDRRTPLATATLLIGANLPDVDVLAYL
                     WGPDTALGFRRGWTHGVPALVLWPFVLTGMMLAWDRWVRRRRRPAAPTAIPRELLLLS
                     AVSILTHPFLDWLNNYGMRWLMPFRDVWTYGDTLFIVDVWIWLALALGVVVSRWRGRR
                     GLTRPERPARLALGAVCVYIALMAGSNLAARGVVRESLTAAGTPPGRLMAGPVPVTPL
                     RRQVVADVGEGYAVGTLDWTGRPAFAPRPGLVTKADGHPAVAAAAATREGAIFLHWAR
                     FPYFVVEERPAAWVVRMGDARYTLQPGQGFGGLTVTVPKETTR"
     gene            2342..2800
                     /locus_tag="VEL74_04040"
     CDS             2342..2800
                     /locus_tag="VEL74_04040"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HYG61729.1"
                     /translation="MPSSPSFQPRDPGYVERVRASFGRQTFMRFLGAELTLVEPGYCE
                     VRLPFRGDFTQQHGYFHAGTLTSIADSAGGYAGFTLMGADDSVLAVEFKVNLLTPARG
                     ELAIARARVVKAGRTLTVCQVDVSTVEAGKETLCAIMQQTLIRLADRPDL"
     gene            2820..3338
                     /locus_tag="VEL74_04045"
     CDS             2820..3338
                     /locus_tag="VEL74_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYG61730.1"
                     /translation="MRPITAADVMNPRVLTVRARMTVRELAHFLMENEISGAPVVDED
                     SKLVGVVSVTDITFALAEEDEDGEDGEEAEEGEDGVEDEGDRTASDFFRDAWGESLRR
                     AEIEDLELEDELEVREIMTPEVHTVPEETPIPEVAELMIRNHVHRLLVTREDKVVGIL
                     STSDLLGLLVRE"
     gene            3413..>3965
                     /locus_tag="VEL74_04050"
     CDS             3413..>3965
                     /locus_tag="VEL74_04050"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HYG61731.1"
                     /translation="MSQRQIPTVNLSDYTAGDAAARARLIQTLGDGLREFGFLNVEGH
                     GIDSELIRGTYQLWKRFFALDEPTKRQYSGIEGGARGFTPFGVEHAKDYPLPDLKEFW
                     HVGQELPEAHPYRYEYPTNVWPSELPEIKDPTLRLYTSLERVAENLLRALAEYFDLPR
                     DTFASMMEWGNSILRVIHYPPVPP"
BASE COUNT          644 a         1421 c         1336 g          564 t
ORIGIN      
        1 cctcaacgag atcgagcaga aggagaagtt cgaccgcctg ttcctcgtcg gctcccagga
       61 gaccatgacg gagatcgaga acgccctccc cgcccccctc gccgccaagc tcgccggcaa
      121 gcagaacgtc gacttcagcg ccggcgacga ccagatcttc gacgcctcgt tcgacatgca
      181 cttcgccgcc gagcgcgagg ccgagtccca gctctgggag aagatcaagg gggagtactg
      241 ccgcggcggc ctcgccgtca ccggcgccga cgaggttctc aaggccgccg ccgtggggcg
      301 cgtcgagaag atggccgtca cccgcgacgc ccgcatctcc ggcacccgct gccgcgagtg
      361 cgagaacctg tcgaacggag agctgtcgaa gtgcccgatc tgcagctcca ccgaagtctt
      421 caaggtggac ctggtcaacg agctggtgga gctcctcgcc ctctccagcg ccgacaccga
      481 cttcgtcgac cccattgagg gcttgagcca ggcgggggac gtcgcggcgc tgctgcgcta
      541 ctagacagaa aagtgtaggg gaggggcttg cctctcccct acatcaggcc atcagctcgc
      601 cggcagcggc ttcgacaggt cgaaggacga ccggaaccgc ttctcgtccc cgcggtcgag
      661 ggagtaacgg aactcctggg cggcggggtc gatttccagg gtccaggcgt tgcctccggc
      721 atggggaagg agcttccggg tgaattcgtc ggtcgggaag gtctggcgca gggcggtgcc
      781 ggtgtcgtcg gagtcgccgc cgtagttggt gacctcctcc ggcgtgccgt cctcgtgccg
      841 gtgatcgtgc ttgaggcgca ggcggtcccc ttccagcgtc agcacccagg tgcgggaacg
      901 cacctccccc acctggaagg ggatgcggat ctccttctcg ctgcaggacg ccacgtgcat
      961 caccaggcgc tggccctcga agtccttgga ggtggtgaac aggaccttcc cctcatagct
     1021 cttgccgcag tgggccgcga ggttctggaa gaacgcctgg gccgggctcg ccggagccgg
     1081 cgcctccgct cctccggccc tcaagggggc caggagaaga accaggggga agagggctcg
     1141 ccaggggcgc tggattctca tcgtgtcgtc tccttgggga cggtgacggt caacccgccg
     1201 aagccctgcc ccggctggag agtgtaacgg gcatcgccca tccggaccac ccaggcggcg
     1261 ggcctctctt cgaccacgaa gtagggaaag cgcgcccaat ggaggaagat cgccccctcc
     1321 cgggtggcgg cggccgcggc caccgccggg tggccgtccg ccttggtcac cagacccgga
     1381 cgcggcgcga aggccggacg gccggtccag tccagggtgc ccaccgcgta gccctccccc
     1441 acgtcggcca ccacctgccg gcgcagcggc gtgaccggca ccggccccgc catcagccgc
     1501 cccggcgggg tgcccgcggc tgtcaggctc tcccggacca ctccccgggc ggcgaggttc
     1561 gagccggcca tcagcgcgat gtagacgcag accgcaccca gggccagccg cgccgggcgc
     1621 tcgggacgcg tgagcccccg ccgtccccgc caacgggaca ccaccacccc cagcgccagg
     1681 gcgagccaga tccagacgtc gacgatgaac agcgtgtcgc cgtaggtcca gacgtcacgg
     1741 aacggcatca gccagcgcat gccgtagttg ttcaaccagt cgaggaaggg gtgggtgagg
     1801 atcgagaccg ccgacaggag cagcaactcg cgagggatgg ccgtgggcgc agcgggccgc
     1861 cggcgccggc gcacccagcg gtcccaggcg agcatcatcc cggtgaggac gaagggccag
     1921 aggacgaggg cgggcacgcc gtgggtccag ccgcgccgga agccgagggc ggtgtccggt
     1981 ccccagaggt aggccaggac gtccacgtcc gggaggttgg cgccgatgag cagggtggcg
     2041 gtggcgagag gcgtccgccg gtcgaggccg gccttgccca gggccgcgcc caccagcgtg
     2101 tggcagaggt tgtccatggc gccgagagct taccccgaca gcaagatttt gatagcgcgc
     2161 ccgccagccc taaggtagcg ttgccctcct tcctccccac cgctactcgc cgccgcggcc
     2221 ctctcccggg ccagcggccg aaggcccggt ggatgtccag ggtgggacct gccgggtcgg
     2281 cgggcccacc ccttttttta cccgcccctg tgacaaaatc cggcctggag acccctcgct
     2341 gatgccttcg tcgccttcgt tccagccccg cgatcccggc tatgtcgagc gcgtccgcgc
     2401 cagcttcggc cgccagacgt tcatgcgctt cctgggcgcc gagctcaccc tggtcgagcc
     2461 cggctactgc gaggtccgcc tccccttccg cggcgacttc acgcagcagc acggctactt
     2521 ccacgccggc accctgacct ccatcgccga cagcgccggc ggctacgcgg gcttcaccct
     2581 gatgggcgcc gacgactcgg tgctggcggt ggagttcaag gtcaacctcc tcacccccgc
     2641 ccgcggcgag ctggccatcg cccgcgcccg cgtggtcaag gccggccgca ccctcaccgt
     2701 ctgccaggtg gacgtctcca ccgtcgaggc ggggaaggag acgctctgtg ccatcatgca
     2761 gcagacactg atccggctgg cggaccggcc ggacctgtag agacgaggag accgccccga
     2821 tgaggcccat caccgccgcc gacgtcatga atccccgcgt gctcaccgtg cgggcgcgca
     2881 tgacggtgcg cgagctcgcc cacttcctga tggagaacga gatctcggga gcccccgtgg
     2941 tcgacgagga cagcaagctg gtcggcgtgg tctccgtgac cgacatcacc ttcgcgctgg
     3001 ctgaagagga cgaggacggc gaagacggtg aggaggccga ggagggagag gacggggtgg
     3061 aggacgaggg ggaccgcacg gcctccgact tcttccgcga cgcctggggc gagagcctga
     3121 ggcgggcgga gatcgaggac ctcgagctcg aggacgagct cgaggtgcgg gagatcatga
     3181 cccccgaggt ccacaccgtg ccggaggaga cccccatccc ggaggtcgcc gagctgatga
     3241 tccgcaacca cgtccaccgc ctgctggtca cccgcgagga caaggtggtc ggtatcctca
     3301 gcacctcgga cctcctcggc ctcctggtcc gggaatgacc tgccggagag gcctgctggt
     3361 aatcttttcc cgttcgtcgt cttctcgatt ccactgagcg gagagcccac ccatgagcca
     3421 gagacagatc cccaccgtca atttgagcga ctacaccgcc ggtgacgccg ccgcccgcgc
     3481 ccggctgatc cagaccctgg gcgacggcct gcgggagttc gggttcctca acgtcgaggg
     3541 gcacggcatc gactccgagc tgatccgcgg cacgtaccag ctctggaagc gcttcttcgc
     3601 cctcgacgag ccgaccaaga ggcagtactc gggcatcgag ggcggcgccc gcggcttcac
     3661 ccccttcggc gtcgagcatg ccaaggacta tccgctcccc gacctcaagg agttctggca
     3721 cgtcgggcag gagctgccgg aggcccaccc ctaccggtac gaatacccga ccaacgtctg
     3781 gccttccgag ctccccgaga tcaaggaccc gacgctgcgc ctctacacga gcctggagcg
     3841 ggtggccgag aacctcctgc gcgccctcgc cgagtacttc gacctgccgc gcgacacctt
     3901 cgcctccatg atggagtggg gcaacagcat cctgcgggtg atccactacc cgccggtccc
     3961 ccctg
//
LOCUS       DASPJI010000153         2952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_180475, whole genome shotgun sequence.
ACCESSION   DASPJI010000153 DASPJI010000000
VERSION     DASPJI010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180475"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2197)
                     /locus_tag="VEL74_04055"
     CDS             complement(<1..2197)
                     /locus_tag="VEL74_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYG61732.1"
                     /translation="MELISTHIGADFDGFAAMIAVRKLHPGARLFFPGSREESLRRML
                     EGRRMEVEELRHKDVDPAALTRLILCDIRQRDRIGVLAEWLEANPGIEVWAYDHHPDA
                     ESDLPVRGGCVDPAAGSASTIVVEEIRRRGLGVTSEEADLLLMGIYEDTGSLTYATTG
                     PRDLEAAAWLLGQGGDLAVVRRYVTRPMDPARLDILHRMTQRLEVHRLRGHRVGLVEV
                     ELEEYVEELAPLVSRCLEIFELPLLFALFAEGGTDRVTLIARGDLPGFDLGQTLAELA
                     GGGGHATAAAARLRGATALEVRERLLGHLRRSLPPAARARDLMITPFYLLPTGTVIEE
                     AKRQLNTRRINAAPVIGARPGGKVVGIVSRQVLDAALQHGLGARPVETVMAPELEWVA
                     PDASAEEVGERMLTRHPRFVLVGDPAAGRPLGVVTRMQLLRHLHGQLADFEERVDRRA
                     EQRRERREQVAHRLARLPEALRSRIAQVAEVSREEGVPVYLVGGLVRDLLLERENRDL
                     DLVVEGDGPRFARRLAERLGGRARVHPAFLTAVVVDREGFHIDVATARSEFYRTPAAL
                     PEVQLSALRQDLFRRDFTINTLAIRLGPDETPELIDYFGGRLDLKEKTLRVLHSLSFI
                     DDPTRVLRAVRLELRLGFQISAETLRLVEVAVAEGVFGLLSGARLREELALLLGDPAL
                     APRGLERLAELGVLRALHPRLELGEAVRQRLRETRAALDWYRLAGIAEPP"
     gene            2248..2862
                     /locus_tag="VEL74_04060"
     CDS             2248..2862
                     /locus_tag="VEL74_04060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG61733.1"
                     /translation="MDWSEFWIEHAGERRDAGTFGPTFLPELLRVIRQYAPQARHFLE
                     WGAGLSTLLFARLAAERQGSVVTIDHESTYARSVMARLGSRAPVRSITADLTGPKLSQ
                     ADPELNYSTLPLSLGGSFDFVFIDGRRRVECALTAFILALPGTIVVLHDYRRARYQPV
                     AVLFETLEAGEHFRVLRRRFDLGRCIEEERGLLMRRLANGSGPS"
BASE COUNT          414 a         1116 c         1037 g          385 t
ORIGIN      
        1 ccggcggctc ggcgatcccg gccagccggt accagtccag cgccgcccgc gtctcccgca
       61 gccgctgccg caccgcctcc cccagctcca gccggggatg gagcgcgcgc aggaccccca
      121 gctccgccag ccgctcgagc ccgcgcggcg ccagggccgg atcaccgagg agcagcgcca
      181 gctcctcgcg cagccgggcg cccgacagca ggccgaagac cccctcggcc accgccacct
      241 cgaccagccg cagggtctcc gccgagatct ggaagcccag ccgcagctcc agccgcaccg
      301 cccgcagcac ccgcgtcggg tcgtcgatga aggacaggct gtgcagcacc cgcagcgtct
      361 tctccttgag gtccagacgc ccgccgaagt agtcgatcag ctccggcgtc tcgtccggcc
      421 cgaggcggat tgccagggtg ttgatggtga agtcgcgccg gaagaggtcc tggcgcaagg
      481 ccgagagctg gacctcgggc agcgccgcgg gcgtccggta gaactcgctg cgcgcggtgg
      541 ccacgtcgat gtggaagccc tcgcggtcca ccaccacggc ggtgaggaag gcgggatgca
      601 cccgcgcccg gccgccgagc cgctccgcca accgccgggc gaagcgcgga ccatccccct
      661 ccaccaccag gtcgaggtcg cggttctcgc gctccagcag caggtcgcgc acgaggccgc
      721 ccaccagata gaccggcacc ccctcctcgc gggagacctc cgcgacctgc gcgatgcgcg
      781 atctcagggc ctccggcagg cgcgccaggc gatgggcgac ctgctcccgc cgctcgcgcc
      841 gctgctcggc ccggcggtcg acgcgctcct cgaagtcggc gagctggccg tgcaggtgcc
      901 ggaggagctg catgcgggtg accaccccca ggggacggcc ggccgcggga tcgcccacca
      961 gtacgaaccg ggggtgacgg gtgagcatgc gctcccccac ctcctcggcg gacgcgtcgg
     1021 gggcgaccca ctccagctcc ggcgccatca ccgtctcgac cgggcgcgcg cccagaccgt
     1081 gctggagcgc cgcgtccagc acctgccggc tgacgatgcc caccaccttg cccccgggcc
     1141 gcgccccgat caccggcgcg gcgttgatcc gccgggtatt gagctgccgc ttggcctcct
     1201 cgatcacggt gccggtgggc agcagataga aaggcgtgat catcaggtcg cgggcgcggg
     1261 ccgccggggg gaggctgcgc cgcagatggc cgagcagccg ctcgcggacc tccagtgccg
     1321 tcgcccccct gagccgggcc gccgccgcgg tggcgtggcc gccgccgccg gcgagctcgg
     1381 ccagcgtctg gcccaggtcg aacccgggga ggtcgccgcg ggcgatgagc gtcacccggt
     1441 ccgtgccgcc ttcggcgaac agggcgaaga gcagggggag ctcgaagatc tccaggcagc
     1501 gcgagaccag gggggccagc tcctcgacgt actcctccag ctcgacctcc accaggccga
     1561 cgcgatggcc gcggaggcgg tgcacctcca gccgctgggt catgcgatgg aggatgtcga
     1621 ggcgggcggg gtccatgggg cgggtgacgt agcgccggac cacggcgagg tcgcccccct
     1681 ggcccaggag ccaggccgcg gcctccaggt cgcgcgggcc ggtggtggcg taggtgagag
     1741 accccgtgtc ctcatagatc cccatgagga gcaggtccgc ctcctcgctg gtgacgccga
     1801 gcccccgccg ccggatctcc tccaccacga tcgtcgacgc cgaccccgcc gccggatcga
     1861 cgcacccgcc ccgcaccggg aggtccgatt ccgcgtccgg gtggtggtcg taggcccaga
     1921 cctcgatgcc ggggttcgcc tccagccact cggccaggac gccgatgcgg tcgcgctgcc
     1981 ggatgtcaca gaggatcagg cgggtgaggg cggcggggtc cacatccttg tgccgcagct
     2041 cctccacctc catgcgccgc ccttcgagca tgcggcgcag cgactcctcc cgtgagcccg
     2101 gaaagaagag ccgcgccccg ggatggagct tgcgcaccgc gatcatggcg gcgaacccgt
     2161 cgaagtcggc cccgatatgg gtggagatca gctccatggg tggattgtag cggcgcggga
     2221 gagaccgggc gggataggat cgcccgcgtg gactggtcag agttctggat cgagcacgcc
     2281 ggggagcgcc gggacgcggg caccttcggt ccgacgttcc tccccgagct gctccgggtg
     2341 atccggcaat atgcgccgca ggcacgacac ttcctggagt ggggcgccgg gttgtcgacc
     2401 ctcctgttcg cccgcctggc tgccgagcgc caggggagcg tcgtcaccat cgaccacgag
     2461 tcgacctacg cgcgctcggt gatggcgcgg ctcgggtccc gggcgccggt gcgctcgatc
     2521 accgcggacc tcaccggccc caagctctcg caggcggatc ccgagctcaa ctacagcacc
     2581 ctccccctct ccctcggcgg gagcttcgac ttcgtcttca tcgacgggcg caggcgggtc
     2641 gagtgcgcgc tgaccgcgtt catcctcgcg cttccgggca ccattgtcgt gctccacgac
     2701 taccggcgcg cccgctatca gccggtagcc gtcctcttcg agaccctcga agccggcgag
     2761 cacttccggg tcctgcgacg ccgcttcgac ctcggccgct gtatcgagga ggagaggggg
     2821 ctcctgatgc gccggctcgc gaacggcagc ggcccgtcct gaaactctgt cgattccggc
     2881 cggcctcgtc cgtcttcaag gtcgaggcgg ttgaaacaga tcggccgctg gacctaggat
     2941 ggcggcccgc aa
//
LOCUS       DASPJI010000154         3169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_180676, whole genome shotgun sequence.
ACCESSION   DASPJI010000154 DASPJI010000000
VERSION     DASPJI010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180676"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..921
                     /locus_tag="VEL74_04065"
     CDS             352..921
                     /locus_tag="VEL74_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61734.1"
                     /translation="MKRVVELVLGALLLVGLGKVAPAQEAPEPSSIHIRPSVGPEEQA
                     RRDAEGKRRWERIRREVEGWRRRWDPAFRPLRRTVEEALGSLRITWGPYSRNLGYAVL
                     EEVARLRGQALLPAPDADLDARLRRALAELEQGADFCLRGMPTRAQLRWEQAIRRLEE
                     ALAPFAPGAAPRPEGPRRIKLVANGQRAQ"
     gene            complement(1018..1094)
                     /locus_tag="VEL74_04070"
     tRNA            complement(1018..1094)
                     /locus_tag="VEL74_04070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1058..1060),aa:Arg,seq:ccg)
     gene            complement(1207..1282)
                     /locus_tag="VEL74_04075"
     tRNA            complement(1207..1282)
                     /locus_tag="VEL74_04075"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1247..1249),aa:Arg,seq:tct)
     gene            complement(1332..1405)
                     /locus_tag="VEL74_04080"
     tRNA            complement(1332..1405)
                     /locus_tag="VEL74_04080"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1369..1371),aa:Pro,seq:tgg)
     gene            complement(1507..>3169)
                     /locus_tag="VEL74_04085"
     CDS             complement(1507..>3169)
                     /locus_tag="VEL74_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYG61735.1"
                     /translation="ARLTGGELNTRRADLRWATGLGGDWYMRLVGGYQQSDDFTRSRN
                     QTVEYSRVCVSTSDTNCLRREAVPLAETEAQIGYGGLRFDKHFESGRVLTIEGGTASL
                     EGPTFVTGIGRVQVTDVQRPWTRVNFNTQHWNVLAYYDQRVAEDQVALSSGALLFEDS
                     SNLHGEIQGNTGFAGGRGFLVGGLAYHQQEVDTSNDQGFHTLMAEAKEEDQQAAFGQV
                     EYNLTDKLKAVVAARVDESTLHDTQFSPKASLVYGFTPNHSLRLTYNEAFQVPNYSEF
                     FLRAPAGAITAFAPLEAALAPLLGGRTLGLASIPILARGNSDLEVEEITSYELGYTAI
                     VSRKLFLTLDYYRNEVENFVTDLLPGVNPVFTTPYAPPSFLPAAVQAQILATLRGGLG
                     QNTFPGLTTINGFPALVLSYTNSGVVDTQGIDFTFNYYVNDRWSTDFSYSWFDFEVQE
                     QDQRDQLLPNAPENKVSAGLSYSGGSFDAGIKYRWVDDFPWAAGIFVGEVPSYDLVDL
                     SVGYRFSDNIKVGVDVSNALDEEHFQAFGGDLIERRALGYVTLGW"
BASE COUNT          490 a         1071 c         1112 g          496 t
ORIGIN      
        1 gccccaccct ttccagatac atctccaggt tccgccgccg ccccgccgcc gcccccggcc
       61 ggtcgagatc cgccaccgcc tctcgatagg ggttgatcac acccggggcc tcgccgcgcg
      121 ggccgcggta gaggaggcgc aggacggggg tgaagtcggg ggtctgcatg tctcggccgc
      181 ccgcgcatcc tcccccagcc ggaggccggt ccgcaacccg tatttcatgg accacgaaat
      241 atgcaagatt tttcccgcgg aatccgtctt tcccctcccg aacgaagccc cgcgcccgga
      301 ccgtgcctcc gcacggcccc cggacggggc gatccctgga gggaagaacg catgaagaga
      361 gtcgtcgagc tggtgctggg agcgttgctg ctggtgggac tcgggaaggt ggcgccggcc
      421 caggaggcac ctgagccgtc ctcgatccat atccggccga gtgtcggccc cgaggagcag
      481 gcgcggcgcg acgccgaagg gaagcggcgc tgggagcgga tccgccggga ggtggagggg
      541 tggcgccggc gttgggaccc ggccttccgg cccctccgcc ggaccgtcga agaggcgctg
      601 gggagcctgc ggatcacctg ggggccgtac tcccgcaacc tcggctacgc cgtgctggag
      661 gaggtcgccc gtctgcgcgg ccaggccctc ctccccgccc ccgacgcgga cctcgacgcc
      721 cgcctgcgcc gtgccctggc cgagctggag cagggggccg atttctgtct gcgcggcatg
      781 cccacccgcg cccagctccg ctgggagcag gcgatccgcc gcctggagga ggcgctggcg
      841 cccttcgccc cgggcgccgc gccacggcca gaaggaccgc gccggatcaa gctcgtggcg
      901 aacggccagc gggctcaata gccgcactcg ctcccttccc cggggcggcc gcaccaagcc
      961 ccgggggagg gatctcccct ggcacttctc gtccgagtct cgtcttgaag agggaagtgg
     1021 tgcgccctag aggattcgaa cctctgacct acagctccgg aggctgtcgc tctatccagc
     1081 tgagctaagg gcgcgcaaac gaaatcgttg gatgagtgaa gggggtcgcc ccccgagcac
     1141 cggcgccaca gtccagcgct ctaccctcgg gctccactca ccatgcgcat cgaattgtga
     1201 agaacgtggc ctgcctggag ggattcgaac ccccgacccg cggcttagaa ggccgccgct
     1261 ctatccactg agctacaggc agccgttgct cggctcttcg ccggacgccg ggcagcctgg
     1321 cttcgtcggt atcggggcgc ccagatttga actggggacc ccctgcgccc aaggcaggtg
     1381 cgctaccagg ctgcgctacg ccccgacgaa aacgcgcagg ttgcacagtg tagagaagcg
     1441 cccaggtggt gtcaaccgac cggagcgccc cgcccatctg gcgagacgct ccggggatgt
     1501 ttttccttac cagcccaagg tgacgtagcc cagcgcccgg cgctcgatca ggtcgccgcc
     1561 gaacgcctgg aagtgctcct cgtccagggc gttgctcacg tccacgccga ccttgatgtt
     1621 gtcgctgaac cggtagccga cgctcaggtc caccaggtca taggacggca cctcgccgac
     1681 gaagatgccc gccgcccagg ggaaatcgtc cacccagcga tacttgatgc cggcgtcgaa
     1741 gctgccgccg gagtaggaca gcccggcgct caccttgttc tccggcgcgt tgggcaggag
     1801 ctggtcgcgc tggtcctgct cctggacctc gaagtcgaac caggagtagc tgaagtcggt
     1861 cgaccagcgg tcgttgacgt agtagttgaa ggtgaagtcg atgccctgcg tgtccaccac
     1921 gccggagttg gtgtaggaga gcaccagcgc cgggaagccg ttgatggtgg tgaggccggg
     1981 gaaggtgttc tgcccgaggc cgccgcgcag ggtggcgagg atctgagcct gcaccgccgc
     2041 cggcaggaac gagggcggcg cgtacggggt ggtaaagacc gggttgaccc ccggcagcag
     2101 gtcggtgacg aagttctcca cctcgttccg gtagtagtcc agcgtcagaa agagcttgcg
     2161 cgacacgatg gcggtgtagc ccagctcgta gctggtgatc tcctccacct ccaggtccga
     2221 gttgccgcgc gcgaggatcg ggatgctggc caatcccagg gtgcgaccgc ccaggagggg
     2281 cgccaaggcg gcctcgagag gcgcgaaggc ggtgatcgcg cccgccggag cgcgcaggaa
     2341 gaattcggag tagttgggga cctggaaagc ctcgttgtaa gtcaggcgca gcgagtggtt
     2401 cggggtgaag ccgtagacca gcgacgcctt gggcgagaac tgggtgtcgt gcagcgtgct
     2461 ctcatcgacg cgcgccgcga ccaccgcctt gagcttgtcg gtcaggttgt actcgacctg
     2521 gccgaaggcc gcctgctggt cctcctcctt cgcctcggcc atcagggtgt ggaagccctg
     2581 gtcgttggag gtgtcgacct cctgctggtg gtaggcgagg ccgcccacca ggaagcctct
     2641 gccgccggcg aagccggtgt tgccctggat ctcaccgtgc aggttcgacg agtcctcgaa
     2701 cagcagggcg cccgaggaga gcgcgacctg gtcctcggcg acccgctggt cgtagtaggc
     2761 gaggacgttc cagtgctggg tattgaagtt gacccgtgtc caggggcgct ggacgtcggt
     2821 cacctggacg cggccgatgc cggtgacgaa ggtcggcccc tcgagcgagg cggtgccccc
     2881 ctcgatggtc agcacccggc cgctctcgaa gtgcttgtcg aaacgcaggc cgccgtagcc
     2941 gatctgggcc tcggtctcgg ccaggggcac ggcctcgcgg cgcaggcagt tggtgtcgct
     3001 ggtcgagacg cagaccctgc tgtactccac cgtctggttg cgcgagcggg tgaagtcgtc
     3061 gctctgttga tagccgccca ccagccgcat gtaccagtcg ccgcccaggc ccgtggccca
     3121 gcgcaggtcg gcccggcggg tgttcagctc gccgccggtg aggcgggcc
//
LOCUS       DASPJI010000155         2639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_582103, whole genome shotgun sequence.
ACCESSION   DASPJI010000155 DASPJI010000000
VERSION     DASPJI010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582103"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2639)
                     /locus_tag="VEL74_04090"
     CDS             complement(<1..>2639)
                     /locus_tag="VEL74_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61736.1"
                     /translation="AYVPLDPDYPAERLAYMVEDAAAPLLLAQRHLTDRLPEMPGTRL
                     LFLDELAHPEAGPPPAIPDGAGPDNLLYVIYTSGSTGRPKGVMVRHRGVVNRLLWAAH
                     AYPVTSADRVLQKAPFSFDFSVWECFGTLLSGARLVLARPGGQRDGAYLVRTVVEQGI
                     TLLHFVPAMLQAFLEEEGLEGCHSLRFVFSGGDALAPEAVERFQARMPPRVLLRNQYG
                     PTEISIDVTEWICREERTGRTPPIGRPLGNGRVYVAGPRWELLPVGVPGELLLAGVGV
                     ARGYRGRPDLTAERFVPSPWGDETGARLYRTGDRVRLLADGNLEFLGRVDHQVKIRGY
                     RIEPGEIEAVLALHPAVREAVVVVRQDAAGLRSLLACVVPVPLPGTAAVTAEELRAAV
                     RGSLPDYMVPGAFALLEALPLTPSGKVDRKALERIAPARGEHAPEAEAVAPRTPSEEI
                     LASIFAEVLRHERVGIHDSFFDLGGHSLLATQVVSRVRQAFGVELPIRDLFEAPTVAG
                     LTERIDRLGRIGRLGRAAMAAPPILPVPRQGDLPLSFAQQRLWFLHRLAPDSAVYNVP
                     MAVRLSGRLDAGALAGALHEIGRRHEALRTTFAEREGHPLQVIAPEPRLPLALLDLTD
                     LPAAEREAQVLRRAQNEVLQPFDLEAGPLMRAALLRLGEEEHALLVTLHHIVTDGWSM
                     GIFLRELTALYVGRRLPELPVQYGDFAVWQRAWLSGKVLESQISYWRQRLVGAPARLT
                     LPTDRPRPAVETHRGTSLPVALPPELSRSLTALARREGATPFMVLLAGFAALLGRYSG
                     QDDVVVGTPIAGRNRREIEGLIGFFVNTLALRVELAAGRGFLDLLRQVRRTALDAYAH
                     QDIPFERLVEELVTERGSLEHSP"
BASE COUNT          392 a         1028 c          909 g          310 t
ORIGIN      
        1 gcggcgagtg ctccaggctg cctcgctccg tgaccagctc ctcgaccagg cgctcgaacg
       61 ggatgtcctg gtgcgcgtag gcgtccagcg ccgtccgccg cacctgccgc agcaggtcca
      121 ggaaaccccg gccggccgcc agctccacgc gcagcgccag ggtattgacg aagaagccga
      181 tgagcccctc gatctcccgc cggttgcggc ccgcgatggg ggtccctacc acaacgtcgt
      241 cctggcccga gtagcggccc agcagcgccg cgaacccggc cagcaggacc atgaacggcg
      301 tcgccccctc gcgccgggcc agcgccgtga gcgaccgcga aagctccgga ggcagcgcca
      361 ccggcaggga ggtcccgcgg tgggtctcca ccgccggccg cggccggtcc gtgggcaggg
      421 tgaggcgcgc cggcgccccg acgaggcgct gccgccaata ggagatctgc gactccagca
      481 cctttcccga caaccaggcc cgctgccaga ccgcgaagtc gccgtactgc accggcagct
      541 ccggcaaccg gcggccgaca tagagcgccg tcagctcccg caggaagatc cccatggacc
      601 agccgtcggt gacgatgtgg tgcagggtca ccagcagggc gtgctcctcc tcgcccagcc
      661 gcaggagggc cgcgcgcatc agcggcccgg cttccaggtc gaacggctgc agcacctcgt
      721 tctgggcccg ccgcagcacc tgggcctcgc gctccgccgc cggcagatcg gtgagatcga
      781 ggagggcgag cggcagccgc ggctccgggg cgatcacctg cagggggtgg ccctcgcgct
      841 cggcgaaggt ggtgcgcagc gcctcgtgac ggcggccgat ctcgtggagc gcgccggcga
      901 gcgcgccggc gtccagccgg cccgacagcc gcaccgccat cggtacgttg tagaccgcgc
      961 tgtccggcgc cagccggtgg aggaaccaga gccgctgctg ggcgaaggac agaggcaggt
     1021 ccccctgccg cggcaccggc aggatcggcg gcgccgccat cgccgctcgc ccgagccgcc
     1081 cgatccgccc gagccgatcg atccgctcgg tcagcccggc gaccgtcggc gcctcgaaca
     1141 ggtcacggat ggggagctcc accccgaagg cctgccgcac ccgcgacacc acctgggtcg
     1201 ccagcagcga gtgccctccc agatcgaaga agctgtcgtg gatgccgacg cgctcgtgcc
     1261 gcaggacctc cgcgaagatc gacgccagga tctcctccga cggcgtgcgc ggcgccacgg
     1321 cctccgcctc cggggcatgc tccccacgcg ctggagcgat cctctccagt gccttgcggt
     1381 ccaccttgcc gctcggcgtc agcggcagcg cctccaggag cgcgaacgcc cccggcacca
     1441 tgtagtccgg cagggagcct cggaccgcgg cccgcagctc ttcggcggtg accgcggcgg
     1501 tccccggcag cggcaccggc accacgcagg ccagcagcga ccgcagcccc gccgcgtcct
     1561 gccgcaccac cacgacggcc tcgcgcaccg cagggtgcag cgccagcacc gcttcgatct
     1621 cccctggctc gatgcgatag ccgcggatct tgacctggtg atcgacgcgg cccaggaact
     1681 cgaggttgcc gtctgccagc aggcgcaccc ggtcccccgt gcggtagaga cgcgcccccg
     1741 tctcgtctcc ccagggggag ggcacgaacc gctccgccgt caggtcgggc cggccccggt
     1801 agccccgcgc cacccccacc cccgccagca gcagctctcc cggcacgccg accggcagca
     1861 gctcccatcg cggcccggcg acgtagaccc ggccgttgcc tagaggacgg ccgatgggcg
     1921 gggtcctgcc ggtccgctcc tcacggcaga tccactcggt cacgtcgata gagatctcgg
     1981 tggggccgta ctgattccgc agcagcactc tgggaggcat ccgggcctgg aaccgctcca
     2041 cggcctcggg tgccagggcg tcgccgcccg agaagacgaa gcgcaggctg tggcagccct
     2101 ccagcccctc ttcctccagg aaggcctgca gcatcgcggg cacgaagtgc agcagcgtga
     2161 tcccctgctc gaccaccgtg cggaccaggt aggcgccgtc acgctggccg cccgggcggg
     2221 ccagcaccag ccgcgccccg gacagcagcg tgccgaagca ctcccagacc gagaagtcga
     2281 agctgaacgg cgccttctgc agcacccggt ccgccgacgt caccggatag gcgtgcgccg
     2341 cccagagcag gcggttcacc accccccggt ggcgcaccat cactcctttt ggacggccgg
     2401 tcgagcccga ggtatagatg acgtacagca ggttgtccgg cccggcgcca tcggggatgg
     2461 ccggcggcgg ccccgcctcc ggatgcgcca gctcgtccag gaagaggaga cgggtgcccg
     2521 gcatctccgg cagcctgtcc gtgaggtggc gctgggccag cagcagaggc gccgccgcgt
     2581 cctccaccat gtaggccagg cgctcggccg gatagtcggg gtccaggggg acgtacgcg
//
LOCUS       DASPJI010000156         5337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_180775, whole genome shotgun sequence.
ACCESSION   DASPJI010000156 DASPJI010000000
VERSION     DASPJI010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180775"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VEL74_04095"
     CDS             <1..460
                     /locus_tag="VEL74_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017974720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYG61737.1"
                     /translation="TLGFRPAGEGGNRFRFESGPGGSGTRVDLLASPSAPRGQSAAGT
                     VHHVAFRVADDPAQKAWHELLLERGHRVSPILDRQYFHSIYFREPGGVLFELATDPPG
                     FTWDESVAELGSGLKLPPWLERERFTIEAILPPIRVGAAAEAAGAVGGAA"
     gene            457..1149
                     /locus_tag="VEL74_04100"
     CDS             457..1149
                     /locus_tag="VEL74_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008427292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYG61738.1"
                     /translation="MSGPDVRDIRDIRDVHGGQPVLAAGRPLEEAAGAVIMVHGRGGS
                     AEDILSLAGELERRGLPEGLAYVAPQAAGHTWYPYSFMAPMERNEPGLSSALALLGDL
                     VEHLESEGVPAERIVLLGFSQGACLSLEFAARNARRFGGVVALSGGLIGPPGTPRDYE
                     GDFAATPVFLGCSDIDPHIPKERVDESAEVLGEMGAEVTERIYPGMGHTVNEDELDFV
                     RQLLAGLAAGKG"
     gene            1250..2407
                     /locus_tag="VEL74_04105"
     CDS             1250..2407
                     /locus_tag="VEL74_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG61739.1"
                     /translation="MRTVDESRILSLVRQIYEAVEAPDGWDRLLSALSSSLDAHVGTL
                     DVYDTARKEGNLAASVNLDPRFGLTYAAHFAAKNLWLNHSPHLIRPGRPLTGQMLVPD
                     DVLSRSEFYQDFLRHENLFHLAGARILEKGTLTAHLSLFRPRDREPFGEEELALLDSL
                     LPHLQQAIRLTRRLSEVDALANLSRSVLDRLPIGTLLLDRSGRVAVMNRTAEEIMALK
                     DGLAVTPCGLKAASRKEDEALTCLVRRTTSAAQGKGLWPGGILRVPRPSFRRAFLVEV
                     VPYGAHMVSGDGGCVAVAVFVSDPEAFPAPSHVGCAYGLTVAEEALTVHLAKGETLES
                     AAEALEVSLNTARTHLKHIFAKTGVKRQSDLVRLVLTGITGLRPTSTKPGP"
     gene            complement(2419..3318)
                     /locus_tag="VEL74_04110"
     CDS             complement(2419..3318)
                     /locus_tag="VEL74_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG61740.1"
                     /translation="MICDPPLDRLFLEGLQDLFATKPRRQRPVSAPPAKPADLGRTLL
                     ESHFELIRQRLLALGRRSGLPDHEADEFLSWALSKLVDSEYRILAGWEGRSSFSTYLT
                     VVLVNLMRDYRTHVWGKWRASAAARQRGPEAILLERLLFRDGLSLEEAIRQMQALGVA
                     LSGPDIEKIATGLPERTERRRVDVEELYKVAVDGRVEERVEMAESARTAAKLRDVLLP
                     LLHTLSAESRVLLKLYFRDGLTMAAVSRVLGRPQKELYRFRDRCLDTLRRHLEDAGLG
                     AREVGALIGTLSDLLVDEAGFWD"
     gene            3889..4092
                     /locus_tag="VEL74_04115"
     CDS             3889..4092
                     /locus_tag="VEL74_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I lanthipeptide"
                     /protein_id="HYG61741.1"
                     /translation="MNKKVKKKMSLSRETLRNLEDTRLQAAAGGVTLGGTNPCSECTI
                     CLSCFDTCMQETMPCMSCDNLCR"
     gene            4237..>5337
                     /locus_tag="VEL74_04120"
     CDS             4237..>5337
                     /locus_tag="VEL74_04120"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HYG61742.1"
                     /translation="MSPASRWQPLLPEDLACKAMDLAEEIAVASEELPPVLRRYRPGD
                     RVALTSGWAGRALFLAYLEQARPGRGWGDRAVELLDQAIEELSEVPADPGLYAGFTGV
                     AWVLEHLQGRLIDGSEDPGEEIASAVEGALRASARPGGFDLISGVAGLGVYVLERGAR
                     PGARDCLCQVVDYLAQTADRRDGTAGWRTPPDRVPVDRKELFPDGYYFTGVAHGVAGV
                     IALLAEAQAAGVEARPLLDEAVTWLLRHKLPAGEGPVYPAEVAPAAEPRPTRLAWCHG
                     DPGIAAALLGAARRAGVPEWEGEAVALARHSAGRSAAERNITDASLCHGSAGLMHLFN
                     RLFQATGEPGLADAARFWFDRTLELRRPGQGVA"
BASE COUNT          813 a         1783 c         1905 g          836 t
ORIGIN      
        1 gaccctgggt ttccggccgg cgggggaggg gggcaaccgc ttccgcttcg aatcgggacc
       61 gggcggctcc ggcacccggg tggacctgct ggccagcccc tccgcgccgc gcggccagtc
      121 cgccgcgggc acggtccacc acgtggcctt ccgggtggcg gacgacccgg cgcagaaggc
      181 gtggcacgag ctgctcctgg agcgggggca tcgcgtgagc ccgatcctgg accgccagta
      241 cttccactcc atctacttcc gggagccggg cggggtgctg ttcgagctgg cgaccgatcc
      301 gccgggcttc acctgggacg agagcgtggc cgagctgggg agcggtctga agctgccgcc
      361 ctggctggag cgcgagcgct tcaccatcga ggcgatcctg ccccccatcc gggtgggggc
      421 ggcggccgag gctgctggag cggtgggagg tgcggcgtga gcgggcccga tgtccgcgac
      481 atccgcgaca tccgcgacgt gcacggcggg cagccggtcc tcgcggccgg ccgcccgctg
      541 gaggaggccg ccggcgcggt gatcatggtc cacggacggg gcggctcggc cgaggacatc
      601 ctctccctcg cgggcgagct ggagcgccgc ggcctgcccg aggggctggc ctatgtggcg
      661 ccccaggcgg cgggacacac ctggtacccg tacagcttca tggcccccat ggagcgcaac
      721 gagccggggc tgtcctcggc gctggccctc ctcggggatc tggtggagca tctcgagagc
      781 gagggggtcc cggcggagcg catcgtcctc ctcggcttct cccagggggc ctgcctgagc
      841 ctcgagttcg cggcgcggaa cgcccgccgc ttcggcggcg tggtggcgct ctccggcggt
      901 ctcatcggtc cgccgggcac gccgcgggat tatgaggggg acttcgccgc caccccggtg
      961 ttcctcggct gcagcgacat cgacccgcac atccccaagg agcgggtgga cgagtcggcc
     1021 gaggtgctgg gcgaaatggg ggccgaggtg accgagcgca tctacccggg gatgggccac
     1081 acggtcaacg aggacgagct ggacttcgtc cgccagttgc tggcggggtt ggcggccggg
     1141 aaggggtgag ggccggaaac cggcggcggc gtccgtctta cccgtcaggc atcactctgc
     1201 ggcccgcgcc gtcccggagc gggccgcccg atgccaggcg ggtatggcca tgcgaacggt
     1261 cgatgagagc cggatcctct ccctggtccg acagatctac gaagccgtcg aggctcccga
     1321 cggctgggac cggctcctgt cggccctgtc cagctccctc gatgcccacg tcgggacgtt
     1381 ggatgtctac gatacggcca ggaaagaggg aaacctcgcc gcttcggtca acctcgatcc
     1441 acgtttcggt ctgacctacg cggcccattt cgccgccaag aacctctggc tgaatcacag
     1501 cccccacctc atccggccgg gacgcccgtt gaccggccag atgctggtgc ctgacgacgt
     1561 cctttcccgc tccgagttct accaggattt cctccggcat gagaatctct tccatctcgc
     1621 cggggcccgt atcctggaga aagggactct gacggcccat ctcagcctct tccggccgcg
     1681 ggaccgggag ccgttcggcg aggaggagct ggcgctgctc gacagcctcc tgccgcacct
     1741 ccagcaggcg atccgcctca ctcgccgcct ctccgaggtc gacgcgctcg ccaacctctc
     1801 gaggagcgtt ctggatcggc taccgatcgg aaccctcctg ctcgacaggt cggggcgggt
     1861 tgccgtcatg aacaggacgg cggaggagat catggctctc aaagacggtc tggccgtcac
     1921 tccttgcggc ctcaaggcag cctcgcggaa ggaggacgag gctctcacct gcctcgtgcg
     1981 ccggacgacc tcggcggcgc agggaaaagg attgtggccg ggagggatcc tgagggtgcc
     2041 gcgcccttcg ttccggcgtg ccttcctggt cgaggtcgtc ccttatggtg cgcacatggt
     2101 ctcgggggat gggggatgcg tcgcggtcgc ggtcttcgtc agcgatcccg aagcctttcc
     2161 tgctccttcc cacgtcgggt gcgcctacgg gctcacggtg gccgaggagg cgctgacggt
     2221 ccacctcgcc aagggcgaga ccctggaatc ggcagcggag gcgctggagg tctcgctcaa
     2281 taccgcgcgc acccatctga agcacatctt cgcgaagacc ggcgtcaaac ggcagtcgga
     2341 tctggtgagg ctggtcttga ccggaatcac gggcctgcgt ccgacctcca cgaagccggg
     2401 cccctgagcc gggagcgttc agtcccagaa acccgcctca tcgaccagga ggtccgacag
     2461 ggttccgatc agcgcgccga cctcgcgggc ccccaggccc gcatcctcca ggtgccgccg
     2521 aagcgtgtcg aggcatcggt cacggaaccg gtacagctcc ttctggggcc gtccgagcac
     2581 gcgggagacc gcggccatgg tgagaccgtc gcgaaagtag agcttgagca ggacacggct
     2641 ctcggcggag agcgtgtgga ggagagggag cagcacgtcg cgaagcttgg ccgcggtgcg
     2701 ggccgactcc gccatttcca ccctctcctc cacccgccca tcgacggcaa ccttgtacag
     2761 ttcctctacg tcgactcgcc gtcgctcggt acgctctgga agacccgtgg cgatcttctc
     2821 gatgtccggc cccgagaggg ctaccccaag cgcctgcatc tgccggatcg cctcctcgag
     2881 ggagaggccg tcgcggaaca ggagccgctc gagcaggatc gcctcgggac ctcgctggcg
     2941 ggctgccgcg gaggctctcc acttccccca gacgtgggtg cgatagtccc gcatcaggtt
     3001 gacgaggacc actgtcaggt aggtcgagaa cgagctccgg ccttcccatc ccgccagtat
     3061 ccggtactcg ctgtcgacga gcttggacag ggcccaggag aggaattcgt cggcctcgtg
     3121 gtccggcagg ccgctccgcc ggcccagggc caggagtctc tgccggatca gctcgaagtg
     3181 gctctcgagc agcgttcttc caaggtcggc cggcttggcc gggggggctg ataccggccg
     3241 ctggcgacgc ggcttcgtgg cgaacagatc ctggaggcct tccaggaaca atcgatcgag
     3301 aggaggatca cagatcatct cgcatctcca ggagaggagc gtggctgagt catcgaagcc
     3361 ccgagtggga ttcactccgg ccactctcac tatgaaacgg cggcaaccgc cgagtcgtca
     3421 cccaaatggg tgagaaagaa agagacctcg ccacggctga atgaacaggg ccgcatcgtg
     3481 ccgtcgtagc ggtagggggt agcgagaaaa aagaggccgc cccgaggagc ggccggatta
     3541 cgcggaggag aaccggccgc tgttcgggcg gccggccctg ggaactgttt cgcaagcgtt
     3601 gagagaattt tcagcgcatg atggcaggcg cgccaccgcc catcggatag ccggccttgt
     3661 ctcgaaggat gggatgggcc ctacccggcg gggtgggagg ggcgacgacg aggaggttcc
     3721 atgaagattc ttgcaactgt ttattcgtat gagctaagat tctctcgtcg ttaaccggcg
     3781 atcggccagg gggcacgaag gagagcggga cctctgggat ccttggaaac cctgggacct
     3841 ctcctccagg gtgaaggggg atcagcctat ctacgcaaag gaggagccgt gaacaagaaa
     3901 gtcaagaaga agatgagcct cagccgtgag acgctacgca acctggagga tacccggctg
     3961 caggcggcgg ctggcggcgt caccctgggc ggcaccaatc cgtgctcgga gtgcaccatc
     4021 tgcttgagtt gtttcgatac ctgcatgcaa gagacgatgc cctgcatgag ctgcgacaac
     4081 ctctgccgtt gacgacctgt ccgggagacc gtctggcagg ccgagggtcc gcggctcttc
     4141 cagccggtcc actccgccct gcgtcgttgc ctgtccggcc gcgggagctc tccccggccg
     4201 ggccctttcg tatcgtcata aggagagtct cgaatcatga gccccgcctc gcgttggcag
     4261 cccctgctcc ccgaagatct ggcgtgcaag gccatggacc tggcggagga gatcgccgtg
     4321 gcctccgagg agctgccgcc ggtgctgagg cgctaccggc ccggggaccg cgtagcgctg
     4381 acgtcgggat gggctggcag ggccctgttc ctcgcctatc tggagcaggc gcgcccgggc
     4441 cgcggctggg gggaccgggc ggtcgagctg ctggaccagg cgatcgagga gctgagcgaa
     4501 gtcccggccg atcccgggct ctatgccggc ttcactggag tggcctgggt cctggagcat
     4561 ctccagggcc ggttgatcga cggcagcgag gatcccggcg aagagatcgc gtcggcggtc
     4621 gagggggccc ttcgcgcctc ggccaggccc gggggcttcg atctgatcag tggcgtcgcc
     4681 ggtctgggcg tctatgtcct ggagcgaggg gcgcggcccg gggcgcggga ctgtctgtgc
     4741 caggtggtgg actacctggc acagacggct gacaggcggg acgggaccgc cggatggcgg
     4801 acgccgcccg accgggttcc ggtcgaccgg aaggagctgt tccccgacgg ctattacttc
     4861 accggcgtgg cccatggcgt cgcgggcgtg atcgcgctgc tggcggaggc ccaggcggca
     4921 ggggtggagg cccggcctct cctcgacgaa gcggtcacct ggctgctgcg ccacaagctc
     4981 cccgccggag aaggaccggt ctatccggcg gaggtcgcgc cggccgccga gccgcggccc
     5041 acccgcctcg cctggtgcca cggcgacccg ggcatcgccg ccgccctgct cggtgccgcc
     5101 cgccgggccg gcgtgccgga gtgggaaggt gaggcagtgg ccctggcgcg ccactccgcc
     5161 ggccgcagcg ccgccgagag gaacatcacc gatgcttcgc tctgtcacgg cagcgccggc
     5221 ctgatgcacc tgttcaaccg tctgttccag gccaccgggg agcccgggct cgccgacgcg
     5281 gctcgtttct ggttcgaccg gacgctggag ctgcgccgcc cgggacaggg ggtggcc
//
LOCUS       DASPJI010000157         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_180780, whole genome shotgun sequence.
ACCESSION   DASPJI010000157 DASPJI010000000
VERSION     DASPJI010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180780"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2715)
                     /locus_tag="VEL74_04125"
     CDS             complement(<1..>2715)
                     /locus_tag="VEL74_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG61743.1"
                     /translation="PAVSGVCTVRLDEGAAAIAARPADRPAGGAGPRNLAYTIYTSGS
                     TGRPKGVQVEHRSPLCLLAALEAAVLAPLAGAEPLLASLNAPMIFDASVQQLVQLLAG
                     HALCMIPEEVRADGAALVALLQERRVDLLDCTPSQLRVLVDAGLLDGPGGPRIVLTAG
                     EAVDEPLWRRLARAERTVSFNLYGPTECSVDATVRRIVSGSTRPTIGRPLAGYEVFLL
                     DSGLRPVPPGAPGEICLGGEGLARGYLRRPDLTAERFVPHPFAQRPGERLYRTGDLGR
                     HLPDSGHELEFLGRLDHQVKIRGFRIELGEIEAALLAVAGVREAVVEARQDRAETGPD
                     DRRLVAYVVGDVKADALRQAMRERLPEYMVPAVFVPLAALPLTPNGKVDRKALPAPEW
                     HTGDQEYLAPRTPVEEILAGIWAELLGCERVGAADHFFELGGHSLLATQVMSRLRSTF
                     EIEMPLRTLFEAPVLADLAVRIEVAQRTGTMPPAPPLVPVPRQGPLPLSFAQQRLWFI
                     DQLEPGTSLYNIPVALRIEGPLRLEVLALCLSEIVRRHEALRTVFAAPEGTPIQVIQP
                     ATPFVLPMVDLSGLPESRREPLALSLAGEETSRPFDLACGPLLRGVLLQLDEDDHVAA
                     LTLHHIVSDGWSMGILVREMTALYSAFAEGKPSPLQELPIQYGDFTVWQRHGLQGEAL
                     EAEVSFWRDALRSLPNLELPTDRPRPRVRTSRGGLYEFSLSAEQSAALASLARAHGST
                     LYMVLLGGFAALLSRWTGQDDIVVGSTIASRNRAEIERLIGFFVNTLVLRIDRSGDPD
                     FGQILERVRKFSLSAFAHQDLPFEKLVEELSVERDPSRTPLFQVLFALQNAGFSALSI
                     PGLTLSPVETQTTTAKFDLTLSMTEVSEVLSGFWEYNRDLFDTATVVR"
BASE COUNT          454 a          909 c          975 g          377 t
ORIGIN      
        1 tgcgcactac cgtcgcggtg tcgaacaggt cccggttgta ctcccagaag ccggaaagga
       61 cctcggatac ctcggtcatc gacagggtca ggtcgaactt cgccgtggta gtttgagtct
      121 cgaccggcga gagcgtcagc ccgggtatcg aaagtgccga gaaaccggcg ttctgaagcg
      181 caaacaggac ctggaagaga ggcgtgcggc tgggatcgcg ctcgacggag agttcctcga
      241 ccagcttctc gaaggggaga tcttgatgcg cgaaggccga gagcgaaaat ttccggaccc
      301 tctccaggat ctgcccaaaa tccggatctc ccgagcgatc gatgcgcagc accagggtat
      361 tgacgaagaa gccgatcaga cgttcgatct cggcccggtt acgactggcg atggtcgagc
      421 cgacgacaat gtcgtcttgc cccgtccaac gggagaggag agccgcgaag ccccccagca
      481 ggaccatgta gagagtgctg ccgtgagccc tggcgaggct cgccagagcg gcgctctgct
      541 cggctgacag cgagaactcg taaaggcccc cgcgcgacgt ccggacccga ggtcgtggcc
      601 ggtcggtcgg aagctccagg ttgggaaggc tccgcaaggc gtcccgccag aaggagacct
      661 ccgcttctag ggcctctccc tggagaccgt gacgctgcca gacggtgaag tcgccatact
      721 ggataggcag ctcctgcaag ggggatggtt tgccctcggc gaatgccgag tagagagccg
      781 tcatctcgcg gaccagaatc cccatcgacc agccgtcgct gacgatgtga tgcagggtca
      841 gcgcggcgac atgatcatct tcgtccagct gtagcagcac gccacgcagc agcggaccac
      901 acgccaagtc gaacgggcgg ctggtctctt cgccggctag gctgagggcc aaaggctcgc
      961 gccggctctc cggcagcccc gaaaggtcca ccatgggcag gacgaaaggg gtagccggtt
     1021 ggatcacctg tatcggagta ccctccggcg cggcaaagac cgtgcgcagc gcttcgtggc
     1081 ggcgtacgat ttcgctcagg cagagcgcca gcacctcgag gcgcagcggt ccctcgatgc
     1141 gcagcgccac cgggatgttg tagagcgacg ttcccggctc gagctggtcg atgaaccaga
     1201 ggcgctgttg ggcgaaggaa agggggagag gcccttgccg gggaaccgga actaggggcg
     1261 gggccggtgg catcgtgccg gtccgctgcg ctacctcgat ccggacggcg aggtccgcca
     1321 ataccggtgc ctcgaagaga gtgcgcaacg gcatttcgat ttcgaacgtg ctgcgcaggc
     1381 gcgacatcac ctgcgtcgcc agcagcgagt ggccgcccag ctcgaagaag tggtcggccg
     1441 cccccacccg ctcgcaaccg aggagctcgg cccagatgcc ggcgaggatc tcctcgaccg
     1501 gcgtgcgcgg cgccaggtac tcctggtcgc cggtgtgcca ctccggagcc ggcagggcct
     1561 tacggtccac cttgccgttc ggcgtgaggg gtagcgccgc cagggggacg aagacggccg
     1621 gcaccatgta ctccggcagc cgctcgcgca tggcctggcg cagcgcgtcg gccttgacgt
     1681 cccctacaac ataggcgacc agccgccggt cgtcggggcc ggtctccgcg cggtcctggc
     1741 gcgcctcgac gaccgcctcg cggacgccgg ccaccgccag tagcgccgcc tcgatctcac
     1801 cgagctcgat gcggaagccc cggatcttca cctggtgatc caggcggccg aggaactcca
     1861 actcgtggcc gctgtccggg aggtgccggc cgaggtctcc cgtgcggtag agccgctcgc
     1921 caggtcgctg ggcgaagggg tggggcacga agcgctcggc agtgaggtcg gggcggcgca
     1981 gatagcctct ggccagacct tcgccgccca ggcagatctc gcccggcgcg cccggcggca
     2041 ccggccgcag gccgctgtcg aggaggaaga cctcgtagcc cgccaacggc cggccgatgg
     2101 tggggcgggt ggagcccgac acgatgcggc ggacggtggc gtccactgag cactccgtcg
     2161 ggccgtagag gttgaacgat accgtccgct cggcccgggc gaggcgccgc cagaggggtt
     2221 cgtccaccgc ctcgcccgcg gtgaggacga tgcgcgggcc gcccggtccg tcgagcaggc
     2281 ccgcgtccac caagacgcgg agctgcgacg gcgtgcagtc cagcaggtcc acccgccgtt
     2341 cctgcagcag ggcgaccagg gccgcgccgt ccgccctgac ctcttcgggg atcatgcaca
     2401 gggcgtggcc ggccaggagc tgcacgagct gctgcaccga ggcgtcgaag atcatgggtg
     2461 cgttgaggct ggcgagcagc ggctccgccc cggccagggg cgcgagcacc gccgcctcca
     2521 gcgccgccag aaggcacagc ggcgagcggt gctccacctg cacccccttg ggtcggccgg
     2581 tggagccgga ggtgtagatg gtgtaggcga ggttgcgcgg cccggcgccg ccggcgggcc
     2641 ggtccgccgg ccgtgcggcg atggccgcgg ccccctcgtc gagccgcacg gtgcacacgc
     2701 cggacacggc gggca
//
LOCUS       DASPJI010000158        10603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_352088, whole genome shotgun sequence.
ACCESSION   DASPJI010000158 DASPJI010000000
VERSION     DASPJI010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10603)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10603
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352088"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..9022)
                     /locus_tag="VEL74_04130"
     CDS             complement(<1..9022)
                     /locus_tag="VEL74_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61744.1"
                     /translation="MTVSNPVGGLSREEKALLFERLRERKRSATPAGRIPRRAPGLEP
                     LPLSFAQERLWFLDRLQPGSSFYNMMTALAGHGDLSPAVLEAALGGVVRRHEALRTIF
                     AESAGTPRQVISPPRRLSLPVVDIACLSDAAREAETRRLFDEEAQRPFDLERGPLLRA
                     TLIRRGSTEHVLLLNMHHIVSDGWSKGVLVRDITELYGAALRGEPSPLPELPIQYADF
                     AVWQREWLREEVLERQLAYWRERLAGIPAALDLPADRQRPPVQSFHGGDIPFIVGPEV
                     TGELNVLSRRRESSLFMTLLTAFQVLLARLSGQQDLPVGSPIANRTRTEVAPLIGFFV
                     NTLVLRADLSGDPTFDELLGQVRSRALDAYAHQDLPFERLVEELRPERHLSHNPLFQV
                     MFALQNTPSDSVALPGLALEPMGGDALTAKFDLELVMVEIRDVLASGFLYSTDLFDAA
                     TIRRFAGHFQVLLAALAEDSGRRLSELPLLADAERHQLVEEWNDTRVPGGEARALALF
                     DAQAARTPDAPAVTFQGDVLNYRELARRANALAHRLRASGVGPEVAVGLCLERSVDIP
                     VGILGVWKAGGAYVPIDPVLPQARLSFLLEDSAVPVLVTRERVLPGLPPLGAEVILLD
                     AVESEAAGPPAGEPAPGDLAYMIYTSGTTGQPKAVMVEHGNLASTLGAMRRAFGFGPG
                     DRMPCVAPFSFDIFLFELLGPLLAGGTVVLLPLWPTLEVERLVDELGEATLLHAVPAL
                     MRQVVESARQRSLSVPLRALFAGGDVVPSDLLADLREVFPSARAHVLYGPTETTIICT
                     SWAVPPEGPVRSLLGRPLDNAVVRLCDAVGNLVPTGVAGEIWIGGPGVTRGYWRREEL
                     TAGKFTEVGGERFFRSGDLARRLPDGSLEFLGRIDQQVKVRGFRIELGEIESALARHS
                     EVREAVVTVRDDPSGGGRRLVAYVVPSPRAELAAVPEEAVEHVSQWQALYEETYGRET
                     APADPTFNIEGWNSSYTGQPIPAGEMREWVDHTVERILALAPRRVLEVGCGTGLILFP
                     VAPHTERYRGTDFSRVALEGIRRHLADTGGLPQVELDWRTADDWSGIEPGELDLVVLN
                     SVTQYFPEVEYLVRVLEAAVRVLAPGGKVFVGDVRSLPLLEALHTSVELFQSLPSTAV
                     AELRRRIGRRVVDEEELLLHPAFFQALARHLPRIERVEVLLKRGRHHNELTRFRYDVV
                     LHLAGGGGAPSGPAPLSPPPEDTSLPAVERLLAEAPVPAVELRGLPNARLAKEAAAAG
                     LLARAGQEIDTVEELRRAVAERQDHGIDPEALWSLGTRMGYAVELTVEPGAPFCFGAR
                     LWRDGAAPAWEGPGEAPGADLPWGAYASDPLRGKLARRLVPELRHFLQAELPEYMIPG
                     AFVLLESLPLTAHGKVDRGALPAPELRGGDFVAPRTPLEEVLAEVWADLLGVDRVGIE
                     DNFFELGGHSLLAVQVVSRLRQRLDLELPVRMLFEEPTIAGLAALIEKERQAAGPASA
                     IVPVRRDRMLPLSFEQERMWFLHQLDPGSAAYSISAPLRLRGDLRPALLEETLRAVVR
                     RHEALRTTFPTLGGRPYQSIHAEPRRVLRLIDLRGLPEADREAELRWIVREQALRPFD
                     LVRGPLLRAALVWLAEDEGALLLTLHHIVSDGWSTGILVREVAEVYGGLLRGQEPALP
                     PLPVQYADFAVWQRAWLQGEGLERDLAYWRERLAGEAPVLRLPVDRPRPALPSGHGGS
                     YRFDLPSAAATALESFTRGEAATLFMGGLAIFLALLARYTGQEDLWVGSPVANRDRSE
                     IQGLIGLFVNTLVLRTGVSGEPGFRELVGRCRETALSAYAHQSFPFEKLVEELRPERD
                     PSRTPLFQVMFALQNVPVEALELPGLAVSPLAAGVTASQFDLSLFLWPTAGGLLGSLE
                     YSLDLFDPSTIERMAGHISSLLVGATAEPAVGLWDLPLLSNAEARQLATWSEDASRGP
                     LPDGTLHGLFAAQARRTPEAVALRVGEERLTYAELAAGAAGLASRLRALGVGPEVPVA
                     LWLPRSAGLVTAMLAVLEAGGFYVPLDPGYPADRVAFLLSDSGAQVVVTDRPLVERLP
                     AAETAPRVVFFDEVEDGPVGGEAALAGNLAYLIYTSGSTGRPKAVAVPHGGAVLLGHW
                     AREVFGPAALAGVLASTSTTFDLSVFEIFVPLAWGGTVVLADDALALRGLPAAGEVRL
                     VNTVPSVLTELLRLGGLPASVSTVNLAGEALPRSLADRVYAQPGIERLYNLYGPSEDT
                     TYSTWALVEKQSDRAPAIGRPLTGTRAYVVDGRLGRLPVGVPGELCLAGSGLARGYFG
                     RPELTAERFVPDPFAADPGARMYRTGDLARYREDGALEYLGRLDHQVKVRGFRIELGE
                     VEAALASLPGVEAAVVLVREERPGDRRLAACVAPADLPVAELREQLRRRLPEFMVPTL
                     WSPMESLPRISTGKVDRRALLALTQQGPASRCEGAVPPRTLAEEWLAEIWSEVLRTGA
                     GIGAFDDFFALGGHSLLATQVLARVRDVFGVELPVRVVFEEPTLAGLAARIEAERRTA
                     GPVVSGIVRVPRQGRLPLSFEQEHMWFLHQLDPRSSAYTLATPLRLRGDLRTTLMEET
                     LRTVVRRHESLRTTFPALGGRPHQEIHPAPERVLRQVDLRALPQAVQTAELERRRQDE
                     ILRPFDLAHGPVLRALLIRLADDEGALLLVLHHIVADGWSMGILVREVAEIYASLLQG
                     EAPRLPEPPVQYVDFAVWQRKWLQGEGLRRDLAYWRERLAGVAPVLRLPEDRPRPPMP
                     SGRGASHRIEWPRDLATALEAFARQEGATLFMAGLAAFLGLLTRYTCQEDVWVGSPVA
                     NRDRAEIQDLIGLFVNTLVLRTSTAENPDFTELVRRSRETALGAYAHQSLPFEKLVEE
                     LRPVRDPSRTPLFQVMFVLQNAPVGDLELPGLALEVLSSEEGAVQFDLSCSLRSIDGE
                     LLGSIEYSSDLFDAATIERMGGHLQTLLADLVADPSTRLWEVPLLREAERLQLSERDR
                     VLGLS"
     gene            complement(9019..>10603)
                     /locus_tag="VEL74_04135"
     CDS             complement(9019..>10603)
                     /locus_tag="VEL74_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HYG61745.1"
                     /translation="FLLADSGAPLVLTHTELSPPSPGGDVRVIAIDAEEDGEAPAGGP
                     QGWTELQPENLAYLIYTSGSTGEPKGVMIRHGAAAAFVEWALGAFAERELAGVLASTS
                     LNFDLSVFELFVPLAMGGAVILADDVLHLPALPAAGEVTLVNTVPSALTGMMRLSPLP
                     PSVATVNLAGEPLPASLAEALWQQSGVRRVLNLYGPSEDTTYSTWAAVPGGTPKPLIG
                     KPVGGGRAYLLDRSLRPVPPGVPGEIFLGGAGLARGYLNRPDLTAERFVPDPFAGLTD
                     LNGEPGARLYRTGDLARWRADGELEFLGRTDFQVKVRGFRIEPGEVEAVVSRHDSVRA
                     CAVLVRTDLRGESRLVAYVVPAGGTQEIARDLRRWLRGRLPDHLVPSAFVPLQALPVT
                     AHGKLDRDALPEPAWGGGVEEVYVAPATPLEETMVRICAEVLGLPVERVGVRDNFFVL
                     GGHSLLATQLIARLRDEYQIEVSLEMVFDAADLRELADRIMERELGEAGDMLDELGDL
                     SPEELQELLGRTDAAEEAE"
BASE COUNT         1639 a         3933 c         3737 g         1294 t
ORIGIN      
        1 agctcaggcc cagcacccgg tcccgctccg agagctggag ccgctcggcc tcgcgcagca
       61 agggcacctc ccacagccgg gtcgacggat cggccacgag gtccgcgagc agcgtctgga
      121 gatggcctcc catgcgttcg atggtggcgg cgtcgaacag gtccgagctg tactcgatcg
      181 atcccagcag ctcgccgtcg atcgagcgca gggagcacga caggtcgaac tggaccgccc
      241 cttcttccga ggagaggacc tccagggcga ggcccggcag ctccaggtcc cccaccggcg
      301 cgttctggag gacgaacatg acctggaaca gcggcgtgcg gctcgggtcg cgcacgggcc
      361 gcagctcctc gaccagcttc tcgaagggca ggctctgatg ggcataagcg ccgagcgcgg
      421 tctctcgcga ccgccggacc agctccgtga aatccggatt ttccgcggtg ctcgtgcgca
      481 gcaccagggt gttgacgaag agcccgatga ggtcctggat ctccgcgcgg tcgcggttgg
      541 ccaccggcga gccgacccag acatcctcct ggcaggtgta gcgggtcagc agccccagga
      601 aggcggcgag gcccgccatg aacagggttg cgccctcctg gcgcgcgaag gcttcgagcg
      661 ccgtggcgag gtcccgcggc cattcgatcc ggtggctggc tccgcgtccc gacggcatgg
      721 gcgggcgcgg ccgatcctcc ggcaacctca gcaccggggc cacacccgcc agccgctccc
      781 gccaatacgc gaggtcgcgc cggagcccct ccccctggag ccacttccgc tgccagaccg
      841 cgaagtccac gtactggacc ggcggctccg gcagccgcgg cgcctcgccc tgcagcaggc
      901 tggcgtagat ctccgccacc tcgcgcacca ggatccccat cgaccagccg tccgcgacga
      961 tgtggtgcag gacgaggagc agagcgcctt catcgtccgc cagccggatc agcagcgccc
     1021 gcagcacggg accgtgcgcg aggtcgaacg gccgcaggat ctcgtcctgg cgccgccgct
     1081 ccaactcggc ggtctggacc gcctgtggca gggctcgcaa atcgacctgc cgcagtaccc
     1141 gctccggtgc gggatggatt tcctgatggg gccgtccgcc cagagcgggg aaggtcgtgc
     1201 gcagggattc atgacgccgc accaccgtgc ggagcgtctc ctccatcagg gtagtccgca
     1261 gatctcctct gagccgcagc ggcgtagcca gggtataggc ggacgagcgc ggatcgagct
     1321 gatgcaggaa ccacatgtgc tcctgttcga aggagagcgg cagccgcccc tggcgcggca
     1381 cccgcacgat acctgagacc acggggccgg ccgtccggcg ctccgcctcg atgcgtgccg
     1441 ccaggccggc gagggtgggc tcctcgaaga ctaccctcac cggcagctcg acgccgaaga
     1501 cgtcgcgcac ccgggccagc acctgcgtcg ccagcaacga gtggccgccg agggcgaaga
     1561 agtcatcgaa ggcgccgatt ccggctccgg tcctcagcac ctcgctccag atctccgcca
     1621 gccactcctc cgccagggtg cggggcggca cggccccttc acagcgggaa gctggtcctt
     1681 gctgggtgag ggcgagcagc gcccgccggt ccaccttgcc ggtcgagatc ctgggcagcg
     1741 actccatggg ggaccagagg gtcggcacca tgaactccgg cagccggcgt cgcagttgct
     1801 cccgcagctc ggccaccggc aggtccgcgg gcgccacgca ggcggccaga cggcggtcgc
     1861 cggggcgctc ctcccgcacc agcaccaccg cggcctccac tcccggcagc gaggccagcg
     1921 ccgcctccac ctcgccgagc tcgatgcgga acccccgcac cttgacctgg tggtccagcc
     1981 ggcccaggta ctccagcgct ccgtcctccc ggtagcgcgc gagatcgccg gtccggtaca
     2041 tccgcgctcc cgggtcggcg gcgaacggat cggggacgaa gcgctccgcg gtcagctccg
     2101 gacggccgaa gtagccccgg gcgaggccgc tgcctgccag gcagagctcg ccgggcaccc
     2161 cgaccggcag ccggcccagg cggccgtcca ccacgtaggc ccgtgtcccg gtcagcggcc
     2221 gaccgatggc cggcgcgcgg tccgactgct tctcgaccag cgcccaggtg gagtaggtcg
     2281 tgtcctccga cgggccatat aggttgtaga gccgctcgat cccgggctgg gcgtagactc
     2341 ggtcggccag cgaccgcggc agcgcctcgc ccgccaggtt caccgtcgac accgaggcgg
     2401 gcagcccgcc caggcgcagc agctccgtca ggaccgacgg gactgtgttg accagccgga
     2461 cctcgccggc cgcgggcagg ccacgcaggg cgagagcgtc gtcggccagc accaccgtgc
     2521 cgccccaggc cagggggacg aagatctcga acaccgaaag gtcgaacgtc gtcgaggtcg
     2581 aagccagcac ccccgccagc gccgccgggc cgaacacctc ccgcgcccaa tggcccagca
     2641 gcaccgcgcc gccatgcggc accgcgacgg ccttgggccg gccggtggag cccgaggtgt
     2701 agatcaggta ggcgagattg ccggcgagcg ccgcctcacc tcccaccggt ccatcctcca
     2761 cctcatcgaa gaagaccacc cgcggggccg tctccgcggc gggcagccgc tccaccagag
     2821 ggcgatccgt caccaccact tgggcgccgc tgtcggacag caggaaggcg acgcggtcgg
     2881 cgggatagcc cggatccagg gggacgtaga agccgccggc ctccagcacc gccagcatgg
     2941 ccgtcaccag gccggccgag cgcggcaacc agagggccac cggcacctcc ggccccacgc
     3001 ccagggcgcg gaggcggcta gccagaccgg cggcgcccgc cgccagctcg gcgtacgtca
     3061 gccgctcctc gcccacgcgg agggccaccg cctccggggt gcgccgggcc tgggcggcga
     3121 acaggccgtg cagggtgccg tccgggagcg gcccacggct cgcgtcttcg ctccacgtcg
     3181 cgagctggcg cgcctcggcg ttgctcagca gcggcaggtc ccacaggccg accgccggct
     3241 ccgccgtcgc ccctacgagc agcgaggaga tatgcccggc catccgctcg atggtggaag
     3301 gatcaaacag gtcgaggctg tactccaggg accccagcag cccgccggcc gtcggccaga
     3361 ggaagaggga gagatcgaac tgagacgcgg tgactccggc cgccagcggg ctcacggcca
     3421 gccccggcag ctccagcgcc tccaccggca cgttctggag ggcgaacatc acctggaaca
     3481 gcggcgtgcg gctggggtcg cgctcgggcc gcagctcctc gacgagcttc tcgaagggaa
     3541 agctctggtg ggcgtaggcg ctcagcgcgg tctcccggca gcgccccacc aattcacgga
     3601 agccgggctc acctgagacg ccggtccgca gcaccagggt gttgacgaac agcccgatga
     3661 gcccctggat ctccgagcga tcgcggtttg ccaccggcga gccgacccag agatcctcct
     3721 ggccggtgta gcgcgccagg agggcgagga agatcgccag accgcccata aacaaagtcg
     3781 ccgcctcgcc ccgagtgaag ctttccagtg ccgtggcggc ggccgacggg agatcgaacc
     3841 ggtagctgcc gccatggcca gagggcagtg ccggacgcgg cctgtccacc ggcagccgca
     3901 gcaccggagc ctcgccggcc agccgctccc gccagtacgc caggtcacgc tccagcccct
     3961 cgccctggag ccacgcccgc tgccagacgg cgaagtccgc gtactgcaca ggcaggggcg
     4021 gcagagccgg ctcctggccg cgcagcagac cgccgtagac ctccgccacc tcgcgcacca
     4081 ggatccccgt ggaccagccg tccgagacga tgtgatgcag ggtcaggagc agcgcgccct
     4141 cgtcctcggc cagccagacc agcgccgcgc gcaggagggg gccgcgcacg agatcgaacg
     4201 gccgcagagc ctgctcccgg acgatccacc gcagctcggc ctcgcgatcg gcctccggca
     4261 ggccccgcag gtcgatcagg cgcagcaccc gtcgtggctc ggcgtggatc gactgatagg
     4321 gccggccgcc cagggtcggg aaggtggtcc gcagagcctc gtggcgccgg acgacggcgc
     4381 gtagcgtctc ctccagtaga gccggccgca ggtctccccg cagccgcaag ggggcggaga
     4441 tgctgtaggc ggcggagccg ggatcgagct ggtggaggaa ccacatgcgc tcctgctcga
     4501 acgagagggg cagcatccgg tcgcggcgga ccggcacgat ggccgaggcc ggtcccgccg
     4561 cttggcgctc cttctcgatc agcgccgcca ggccggcgat ggtgggctcc tcgaagagca
     4621 tccgcaccgg tagctccaga tcgaggcgct ggcgcagccg cgaaacgacc tgaacggcca
     4681 ggagagagtg ccctccgagc tcgaagaagt tgtcctcgat ccccacccgg tcgaccccca
     4741 ggaggtcggc ccagacctcc gccagcacct cctccagcgg ggtgcggggc gcgacgaagt
     4801 cgccgccgcg gagctccggg gccggcaggg ccccccggtc caccttgccg tgggcggtga
     4861 gcggcaggct ctccagcagc acgaaggcgc cggggatcat gtactcgggc agctccgcct
     4921 ggaggaagtg gcgcagctcg gggaccagcc ggcgggcgag cttgccgcgc aacggatcgc
     4981 tggcgtacgc gccccagggc aggtccgccc cgggagcctc tcccgggccc tcccaggctg
     5041 gagccgcgcc gtcgcgccag aggcgcgctc cgaagcagaa aggcgcgccg ggctcgacgg
     5101 tcagctcgac ggcatacccc atccgcgttc cgaggctcca cagggcctcg ggatcgatcc
     5161 cgtgatcctg tcgctccgcc accgcccggc gcagctcctc caccgtatcg atctcctgcc
     5221 ccgccctggc gaggagcccg gccgccgcgg cttccttggc caggcgcgcg ttgggcaggc
     5281 cccgcagctc gaccgcgggc acgggagcct ccgccaggag ccgttcgacg gcggggaggg
     5341 acgtgtcctc gggagggggg ctgagcggcg cgggcccgga aggcgcgccg ccgccgccgg
     5401 ccaggtgcag caccacgtcg taccggaagc gggtcagctc gttgtgatgc cgtcccctct
     5461 tgaggaggac ctccacccgt tcgatccgcg gtaggtgccg cgcgagcgcc tggaagaacg
     5521 cggggtggag cagcagctcc tcctcgtcca ccacgcgccg gccgatgcgc cgccgcagct
     5581 cggcgacggc cgtggaaggc aggctctgga acagctccac cgacgtgtgc aacgcctcca
     5641 gcagcggcag gctgcggacg tctcccacga agaccttgcc ccccggcgcg agcacccgca
     5701 ccgcggcttc gaggacccgc accaggtact cgacctcggg gaagtactgc gtcaccgagt
     5761 tcaggaccac gaggtcgagc tcgcccggct cgatcccgct ccagtcgtcg gccgtccgcc
     5821 agtccagctc gacctggggc agcccaccgg tgtccgccag atggcgccgg atgccttcca
     5881 gggccacccg cgagaagtcg gtgccgcggt agcgctccgt gtggggggcg acggggaaca
     5941 ggatcagccc cgtgccgcag cccacctcca gcacccgccg cggcgccagc gcgaggatcc
     6001 gttcgacggt gtggtccacc cactcgcgca tctcccccgc cgggatcggc tgccccgtgt
     6061 agctgctgtt ccacccttcg atgttgaatg tcgggtccgc cggcgcggtc tcgcggccgt
     6121 aggtctcctc gtagagcgcc tgccactgcg agacgtgctc caccgcctcc tcgggcaccg
     6181 ccgccagctc ggcccggggg ctcggcacca cataagccac cagccgccgg ccgccgcccg
     6241 atgggtcgtc ccgcaccgtc accaccgcct cccgcacctc ggagtggcgg gcgagcgcgc
     6301 tctcgatctc gcccagttcg atgcggaagc cgcgcacctt gacctgctgg tcgatgcgcc
     6361 ccaggaactc gaggctgccg tcgggcagcc ggcgggccag atcgccgctg cggaagaagc
     6421 gctcgcctcc cacctccgtg aacttgccgg cggtgagctc ctcgcggcgc cagtagcccc
     6481 gggtgactcc cgggccgccg atccagatct cgccggccac gccggtgggc accaggttgc
     6541 cgacggcgtc gcacagccgc accacggcgt tgtccagggg cctgccgagg agggagcgca
     6601 ccggcccctc cggcggcacc gcccaggagg tgcagatgat ggtcgtctcg gtcggaccgt
     6661 agagcacgtg cgcccgtgcc gacgggaaga cctcgcgcag gtccgccagg aggtccgagg
     6721 ggaccacgtc gccgccggcg aagagggcgc ggagagggac cgagagggac cgctgccgcg
     6781 ccgattcgac cacctgccgc atcagggccg gcacggcgtg gagcagcgtc gcctccccca
     6841 actcgtcaac caggcgctcg acctcgagcg tcggccacag cggcagcagc acgacggtgc
     6901 cccccgcgag cagcggcccg agcagctcga agaggaagat gtcgaaggag aacggcgcca
     6961 cgcacggcat ccggtcgccg ggaccgaagc cgaaggcgcg ccgcatcgcg cccagcgtgc
     7021 tcgccaggtt gccgtgctcc accatcaccg ccttgggctg gccggtggtc ccggaggtgt
     7081 agatcatgta ggccagatcg cccggagccg gctcccccgc cggcggcccg gcggcttcgc
     7141 tctctacagc gtcgaggagg atcacctccg ctccgagggg cggcagcccc gggagcaccc
     7201 tctcccgggt gaccagcacc ggcaccgcgg agtcctccag gaggaagctc aggcgcgcct
     7261 ggggcaggac cggatcgatc ggcacgtagg cgccgcccgc cttccagacc cccaggatgc
     7321 cgacggggat gtcgaccgac ctctccaggc agagccccac cgccacctcc ggacccaccc
     7381 ccgaggcgcg caggcggtgc gccagtgcgt tggcccggcg cgccagctcg cgatagttca
     7441 ggacgtcgcc ctggaaggtc acggccgggg cgtccggcgt ccgcgccgcc tgcgcgtcga
     7501 agagagcgag cgcacgcgcc tctccaccgg gcacccgcgt gtcgttccac tcctccacga
     7561 gctggtgccg ctccgcgtca gccagcagcg gcagctcgga gagccgccgt cccgagtcct
     7621 ccgccagcgc ggcgagaagc acctggaagt gaccggcgaa gcgccggatg gtggcggcgt
     7681 cgaacaggtc tgtgctgtaa aggaaacccg atgccaggac gtcccggatc tcgaccatta
     7741 cgagctccag atcgaacttg gcggtcagcg cgtcgcctcc catgggctca agcgccagcc
     7801 cgggcagagc gacagagtcc gagggcgtgt tctgcagcgc gaacatcacc tggaacagcg
     7861 ggttgtggga gaggtgccgc tcgggccgga gctcctcgac gagacgctcg aacggcaggt
     7921 cctgatgggc gtaagcgtcg agagcgcggc tgcgcacctg cccgaggagc tcgtcgaagg
     7981 tcggatctcc cgaaaggtcg gcgcgcagga ccagagtgtt gacgaaaaag ccgatcaggg
     8041 gggcgacctc ggtccgggtg cggttcgcga tgggtgatcc caccggcagg tcctgttggc
     8101 cgctcagcct ggcgagcaga acctggaagg ctgtgagcag cgtcataaag agggacgact
     8161 cacgccggcg ggagaggacg ttgagctctc cggtgacctc cggtcccacg ataaagggta
     8221 tgtccccgcc atggaagctc tgcaccggcg gacgctgccg gtcggcgggc aggtccaggg
     8281 cggccgggat ccccgccaac cgctcgcgcc agtaagcaag ctggcgctcg agcacctcct
     8341 cgcgcagcca ctcccgctgc cagaccgcga agtcggcgta ctggattggc agctccggca
     8401 ggggcgacgg ctcgccgcga agcgccgcgc cgtacagctc ggtgatgtcc cgcaccagca
     8461 cgcccttgga ccacccgtcg gagacgatgt ggtgcatgtt gagcagcagc acgtgctcgg
     8521 tgctcccccg ccggatcagt gtggcgcgca gcagcggccc acgctccagg tcgaacggcc
     8581 gttgcgcctc ctcgtcgaac agacgtcgcg tctctgcttc gcgggccgcg tcggaaaggc
     8641 aggcgatatc aaccaccggc aggctcaagc gcctgggggg agagatcacc tggcgcggtg
     8701 tgcctgcgct ctcggcgaag atggtacgca gcgcctcgtg gcgccggacc acgccgccca
     8761 acgccgcctc cagcaccgcg ggcgagaggt cgccgtggcc cgccagggca gtcatcatgt
     8821 tgtagaagga gctacccggc tggagccggt cgaggaacca gagacgctcc tgggcgaacg
     8881 acaggggcag gggctccagc cccggcgcgc ggcgggggat ccgccccgcc ggggtagcgg
     8941 acctcttccg ctcccgcaga cgctcgaaga ggagggcctt ctcctcgcgg gagaggccgc
     9001 cgaccgggtt ggagaccgtc actccgcctc ctctgccgcg tccgtccggc cgagcagctc
     9061 ctgcagctcc tcgggcgaga ggtcgcccag ctcgtccagc atgtcgcctg cctcgcccag
     9121 ctcccgttcc atgatgcggt cggccagctc gcggaggtcc gcggcgtcga acaccatctc
     9181 gagcgacacc tcgatctggt actcgtcccg cagccgggcg atgagctggg tggcgagcag
     9241 cgagtgcccg cccaggacga agaagttgtc gcgcacgccg acgcgctcca ccggcaggcc
     9301 gaggacctcg gcacagatcc gcaccatggt ctcctccagc ggcgtcgcgg gcgcgacgta
     9361 cacctcctcc accccgcccc cccaggccgg ctcggggagg gcgtcccggt cgagcttgcc
     9421 gtgggcggtg acgggcagcg cctggagcgg cacgaacgcc gagggcacca ggtgatcggg
     9481 cagccggcca cgcagccagc ggcgcaggtc gcgggcgatc tcctgcgtcc cgccggcggg
     9541 cactacgtag gccaccaggc gggactcgcc gcggagatcg gtccgcacca gcacggcaca
     9601 ggcgcgtacg ctgtcgtggc gggagaccac cgcctccacc tccccgggct cgatgcggaa
     9661 gccccgcacc ttcacctgga aatcggtgcg gccgaggaac tccagctcgc cgtccgcccg
     9721 ccagcgcgcc aagtcgcccg tccggtagag ccgggcaccc ggctccccgt tgagatcggt
     9781 gaggccggcg aagggatcgg gcacgaagcg ctccgccgtc agatcgggcc ggttcagata
     9841 gccgcgtgcg aggccggcac cgccgaggaa gatctccccc gggactccgg gggggaccgg
     9901 ccgcaggctc cggtcgagca gataggcccg cccgccgccc accggcttgc cgatcagggg
     9961 cttgggcgtg ccgccgggga cggcggccca ggtagagtac gtggtgtcct cggacgggcc
    10021 atagaggttg agcacccgcc gcacgcccga ttgttgccag agcgcttcgg cgagggaagc
    10081 cgggagcggc tcaccggcca ggttgaccgt cgccacggag ggcggcagag gggacaggcg
    10141 catcatcccc gtgagggcgg acgggacggt gttgaccagc gtcacctcgc ccgcggcggg
    10201 gagcgccggc agatgcagga cgtcgtcggc caggatcacc gcgccgccca tggcgagggg
    10261 gacgaacagc tcgaacaccg agaggtcgaa gttgagggag gtcgaggcga gcactcccgc
    10321 gagctcccgc tccgcgaagg ctcccagggc ccattcgacg aaggcggcgg cggcaccgtg
    10381 tcggatcatg acccccttgg gctcgccggt ggagcccgag gtgtagatga gataggccag
    10441 gttctccggc tggagctccg tccatccctg gggcccgccc gccggagctt cgccgtcctc
    10501 ctccgcgtcg atggcgatca cccgcacgtc gccgccgggc gacggcgggc tcagctccgt
    10561 atgggtcagg accaggggcg cgcccgagtc cgccagcagg aag
//
LOCUS       DASPJI010000159         4251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_181303, whole genome shotgun sequence.
ACCESSION   DASPJI010000159 DASPJI010000000
VERSION     DASPJI010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181303"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VEL74_04140"
     CDS             complement(<1..755)
                     /locus_tag="VEL74_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HYG61746.1"
                     /translation="MPEAAGELERGGFPAWLAMLLARRGVADGPGAERFLRPSLDQLH
                     DPYLLCGMEEGVDLLLKARERGDCVAVVGDYDVDGVTATALLLAVFGACGLTVRPILP
                     HRLREGYGFQPVHVERAREQGCRVIVTADCGSSSAEAVAAALGSGIDVLVTDHHLPGC
                     DLPAGAVQINPRQPVCSYPYPYLAAAGLAFKLATALAARCGRPLEVAALLRIACLGTI
                     ADLVPLTGENRVIAALGLEALGTARSQGLKALIR"
     gene            complement(802..1755)
                     /locus_tag="VEL74_04145"
     CDS             complement(802..1755)
                     /locus_tag="VEL74_04145"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HYG61747.1"
                     /translation="MQGTDFSFEESLADLRRRVKELEGYPAGSGQERELERLRATLKK
                     STTDAYGHLTRWQKALVARHPERPYTLDYVEHLMSDWVEVHGDRRFADDPAIVSGFAT
                     FRGRSVAVIGHQKGRDTKERIYRNFGQPRPEGYRKALRIMKLAEKFGLPVLTFIDTAG
                     AYPGLDAEERGQAEAIAFNLMEMAVLRVPIVVTITGEGGSGGALALGVGDRVFMLEYA
                     TYSVISPEGCAAILWKDQEKKAEAAEAMRVTAPDLLSLGVVDGIIPEPLGGAHTDPET
                     ACRRVGDTLETSLRELERLPPQELIGRRYEKFRSLGVYDEG"
     gene            complement(1809..>4251)
                     /gene="priA"
                     /locus_tag="VEL74_04150"
     CDS             complement(1809..>4251)
                     /gene="priA"
                     /locus_tag="VEL74_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HYG61748.1"
                     /translation="ALNAPLSAPLYADIALPLPLPDPLTYEVPPTLAAMVRPGVRARV
                     AVGRRRLVGVIVAVHDRTPEGINLRAVQEVLDLEPVVTPDLMELARFTSSYYMAPLGE
                     VFRSMLLSDLPPWGDRRIRLTDSGALALPRNEAEGRVIEVLREEGRMSAGELQARVGL
                     ADLDAVLASLAERGRIVREEPRPSGARYISAVELAPGEVEKHRAAAGRSAPGRAVVDY
                     LDAIGRPATVAEISAAIGSSPAVVRRLVSKGILRQFTQVERRSLERHMFASQEKPAEE
                     ITLRGDQQVALERLTGVIARREFAPLLLQGMTGSGKTEVYLRAAEAALAQGRSVILLV
                     PEIALVPALAREVEQRFGEQLAILHSGLGMGERHQEWERVRRGEARVVLGPRSALFAP
                     VSDLGLLVVDEEQDSSYKQEVTPRYHARDLALVRGRDAGAATLLVSATPSMESRSNAE
                     RGKLEPLRLTARAGHGSLPEGILVDLRQEGLSRRPGEVHFSERLRDEITTALAAGDQV
                     ILLRNRRGYAPLLLCRACGEDMRCEDCGLPRTYHRRARRLVCHYCGSTLAAPQRCPTC
                     SEEALEPIGAGTERVEDEFRELFPGAAVDVLDRDTARRPGGPAALLERFSRGEVQVLI
                     GTQMVSKGHHFPGVALTAVLAADAYLGFPDFRAVERTYNLLTQVAGRAGRGERPGRVV
                     IQTYHPDHYAIQAALHQDDEGFAREELHFRRVFHYPPFTRMVQLLVKDKYRERAQAIM
                     LELSADLAAHPLHRLVRLSGPAPAPLERLRGEWRFQLLVRSAAGRDLHRLLGDVLPRN
                     PPYDLTIDVDPQQLL"
BASE COUNT          621 a         1550 c         1537 g          543 t
ORIGIN      
        1 cggatcagcg ccttcaagcc ctgggaccgc gccgtgccca gcgcttccag gccgagcgcg
       61 gcgatgaccc ggttctcgcc ggtcaagggc accaggtcgg cgatcgtccc caggcaggcg
      121 atccggagca gcgccgcgac ctccagcggc cggccgcagc gcgccgccag agccgtggcg
      181 agcttgaacg cgagcccggc cgcggccaga tacggatagg gataggagca gaccggctgc
      241 cgcggattga tctggaccgc tccggccggc aggtcgcagc cgggcaggtg gtggtcggtg
      301 accagcacgt cgatgccgga gccgagcgcc gccgccaccg cctccgccga ggagctgccg
      361 caatccgcgg tgacgatgac gcggcacccc tgctcgcggg cgcgctcgac gtgcaccggt
      421 tggaagccat agccttcgcg caggcggtgc ggcaggatgg gccgcaccgt caggccgcag
      481 gcgccgaaca ccgccagcag cagggcggtg gcggtgacgc cgtccacgtc gtagtcaccc
      541 accaccgcga cgcagtcgcc gcgctcccgc gccttcagca ggaggtccac cccctcctcc
      601 atgccgcaga gcagataggg atcatgaagc tggtccaggg agggccgcag gaagcgctcg
      661 gctccggggc cgtcggcgac gccgcggcgg gcgaggagca tagcgagcca ggcggggaac
      721 cctccccgct ccagctcgcc ggcggcctcg ggcaccggcg ccgcgaccca gtccgccgcg
      781 gtacccgcgg gagacagcgc tctagccctc gtcgtagacc ccgagggagc ggaacttctc
      841 gtagcgccgc ccgatcagct cctgaggcgg gaggcgctcc agctcccgga gcgacgtctc
      901 cagggtatct ccgacccggc ggcaggccgt ctccggatcg gtgtgggcgc cgcccagcgg
      961 ctcggggatg atgccgtcca ccacgccgag cgacagcagg tcgggagcgg tgacgcgcat
     1021 cgcctcggcg gcctcggcct tcttctcctg gtccttccag aggatggccg cgcaaccctc
     1081 aggcgagatg acggagtagg tggcgtactc cagcatgaag acccggtccc ccacgccgag
     1141 cgccagggcg ccgccgctcc ccccctcgcc ggtgatggtg acaacgatgg gcacccgcag
     1201 aaccgccatc tccatcaggt tgaaggcgat ggcctcggcc tgcccgcgct cctcggcgtc
     1261 gaggccggga taggcccccg cggtgtcgat gaaggtgagc accggcaggc cgaacttctc
     1321 cgccagcttc atgatccgca gcgccttgcg gtagccctcg ggacgcggct ggccgaagtt
     1381 gcggtagatc cgctccttgg tgtcgcgtcc cttctggtgg ccgatcaccg ccacggagcg
     1441 gccgcggaag gtggcgaagc cggagacgat ggcggggtcg tccgcgaagc ggcggtcgcc
     1501 gtggacctcg acccagtcgc tcatcagatg ctcgacgtaa tccagcgtgt aggggcgctc
     1561 cggatgccgc gccaccagtg ccttctgcca gcgggtcaga tggccgtagg cgtcggtcgt
     1621 gctcttcttg agcgtagcgc gcagccgctc cagctcccgc tcttgcccgc tccccgcagg
     1681 gtagccttcg agctctttga cgcggcgccg caagtcggcg agggattctt cgaacgagaa
     1741 atcggtaccc tgcatggcga ccggcaaggt agcaaagccg gctgggcagg tcaacgtccg
     1801 gcctccggtc acaggagctg ctgggggtcg acgtcgatcg tcaggtcgta gggagggttc
     1861 ctgggcagca cgtcaccgag caggcggtgg aggtcgcggc cggcggccga gcggacgagg
     1921 agctggaacc gccactcgcc gcgcaggcgc tccagcgggg ccggcgccgg tcccgacagc
     1981 cgcaccaagc ggtgcagcgg atgggcggcg aggtcggcgg agagctcgag catgatcgcc
     2041 tgcgcccgct cccggtactt gtccttcacc aggagctgca ccatgcgggt gaacggcgga
     2101 tagtggaaca cccgccggaa atggagctcc tcgcgggcga acccctcgtc gtcctggtgc
     2161 agggcggcct ggatggcata gtggtccgga tggtaggtct ggatgaccac ccgtcccggc
     2221 cgctctcccc tccccgcccg gccggcgacc tgggtgagca ggttgtaggt gcgctccacg
     2281 gcccggaaat cggggaagcc gagataggcg tcggcggcga ggaccgcggt cagcgcgacg
     2341 cccgggaagt ggtgcccctt ggacaccatc tgggtgccga tgaggacctg cacctcgccg
     2401 cgggagaagc gctccaggag ggccgccggg ccgccgggcc gccgggcggt gtcgcgatcc
     2461 agcacgtcga ccgccgcgcc ggggaacagc tcgcggaact cgtcctccac gcgctcggtg
     2521 ccggcgccga tcggctccag cgcctcctcg gagcaggtgg ggcagcgctg cggcgccgcc
     2581 agagtcgacc cacagtagtg gcagaccaga cggcgggccc ggcggtgata ggtccgcggc
     2641 agtccgcagt cctcgcaacg catgtcctcg ccgcaggccc ggcagaggag cagcggcgcg
     2701 tacccccggc gattgcgcag caggatgacc tgatcgccgg cggcgagcgc cgtggtgatc
     2761 tcgtcccgca gccgctccga gaagtggacc tcccctgggc gccgcgacag cccctcctgc
     2821 cgcagatcca ccaggatccc ttcgggcagg gagccgtggc cggcgcgggc ggtgaggcgc
     2881 agcggctcca gcttcccccg ctcggcgttg gaccggctct ccatgctcgg ggtggccgac
     2941 accagcaggg tggcggcgcc ggcgtcgcgg ccgcggacca gggcgaggtc gcgggcgtgg
     3001 tagcgggggg tgacctcctg cttgtaggag ctgtcctgct cctcgtccac caccagcagc
     3061 ccgagatcgg agaccggcgc gaacagagcc gagcgcggcc ccagcaccac ccgcgcctcg
     3121 ccgcggcgga cccgctccca ctcctggtgg cgctccccca tgcccagccc cgagtgcagg
     3181 atcgcgagct gctcgccgaa gcgctgctcc acctcccggg cgagggcggg caccagggcg
     3241 atctcgggca ccaggaggat caccgacctc ccctgcgcca gggccgcctc ggctgcccgc
     3301 aggtagacct cggtcttgcc cgaaccggtc atgccctgga gcagcagcgg cgcgaactcc
     3361 cggcgggcga tcacgccggt caggcgttcc agcgccacct gctggtcgcc gcgcagggtg
     3421 atctcctccg ccggcttctc ctgcgaggcg aacatgtggc gctccagcga gcgcctctcc
     3481 acctgggtga actgccgaag gatccctttg ctcaccaggc ggcgcaccac cgccgggctg
     3541 gagccgatag cggccgagat ctcggccacg gtcgccggcc ggccgatggc gtccaggtag
     3601 tcgaccacgg cccgccccgg ggcggagcgg ccggcggcgg cgcggtgctt ctccacctca
     3661 cccggcgcca gctccacggc cgagatgtag cgcgcgccgc tggggcgcgg ctcctcgcgg
     3721 acgatccggc cgcgctcggc gagcgacgcg agcacggcgt cgagatccgc caggccgacc
     3781 cgcgcctgaa gctcccccgc cgacatcctc ccctcctcgc gcagcacctc gatcacccgc
     3841 ccctccgctt cgttccgagg cagggccagg gcgccggaat cggtgaggcg gatgcggcgg
     3901 tccccccagg gtggcaggtc ggacagcagc atcgaccgga acacctcgcc gagcggcgcc
     3961 atgtaataag aggatgtgaa gcgggccagc tccatgaggt ccggggtgac caccggctcg
     4021 aggtccagca cctcctggac ggcacgcagg ttgatcccct cgggagtccg gtcgtgcacc
     4081 gcgacgatga ccccgaccag ccggcgcctc cccacggcca cccgggcgcg cacccccggc
     4141 cggaccatag cggcaagggt ggggggcacc tcgtaggtca gtggatcggg cagcggcagg
     4201 ggcagcgcga tgtctgcgta cagaggggcg gacaacgggg cgttgagagc g
//
LOCUS       DASPJI010000160         5098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_241147, whole genome shotgun sequence.
ACCESSION   DASPJI010000160 DASPJI010000000
VERSION     DASPJI010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241147"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..2217
                     /gene="ligA"
                     /locus_tag="VEL74_04155"
     CDS             166..2217
                     /gene="ligA"
                     /locus_tag="VEL74_04155"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HYG61749.1"
                     /translation="MDREKARERIARLRAEIRHHDFLYYVRDAPEISDAAYDKLYREL
                     VSLEEQFPDLVEPDSPTQRVAGQPLTSFPTVEHAAPMLSLDSDASEASLRRFDERLRK
                     ALPDVEVEYVLEPKLDGASVELVYEDGVLARASTRGDGRRGEGITENVRTIQAVPLRL
                     RTEAPHPVPPFLSLRGEAIMHASVFDKLNERLIAEGKEPFANPRNVAAGALRQLDPQQ
                     TASKPLDLFAYDVLSAGESAQIPGIETQWEVLQALKEWGLRVSSLVRKVKTLEEILEY
                     HQDLEARRDDLGFEIDGIVIKLNDLAARDRVGWTSRHPRWAFAFKFPPRKEITRILSI
                     VPSVGRSGVVTPVALMLPVELGGVTVSRATLHNREEVARKDVREGDRVRIQRAGDVIP
                     QVVERIVEPEREAEREKEPRWQMPATCPSCGTPLIERGPFTICPNGFECPAQLVGRIV
                     HLASRDALDIEGLGDETAKLFVNEGLVRQLPDVFDLRAEQLVHLEGFAEKSSKKLEEA
                     LRKASKVELPRLLHGLGIPEVGVAVARDLSRHFGTFERIRTADAAALQEVAGVGPRMA
                     EAITTFFRDEKNAPILDELLKRLDVQEQSAAPPASVLPLSGKKFVFTGGLAQMSRRDA
                     QNLVESLGAKATGSVSKSTDYVVVGEDAGSKADDARTLGVTTLDEEGFLDLLRSLGAA
                     V"
     gene            2235..2762
                     /gene="msrB"
                     /locus_tag="VEL74_04160"
     CDS             2235..2762
                     /gene="msrB"
                     /locus_tag="VEL74_04160"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HYG61750.1"
                     /translation="MTAKLVTIGIVLAAFLAWHWISPDKPAALEAAERQQKGSKKVDT
                     IEKIVKSDAEWKKEMTSEEFHVMREKGTERAFTGKYWNNHEKGTYLCGACGLELFSSE
                     TKFDSGTGWPSFWQPIDQSHVETETDRAWGMKRVEVHCARCGAHLGHVFEDGPKPTGL
                     RYCMNSVSLDFKKAE"
     gene            2877..3503
                     /locus_tag="VEL74_04165"
     CDS             2877..3503
                     /locus_tag="VEL74_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HYG61751.1"
                     /translation="MSSEPSSIRGIDVSKYQGTIDWSQVATAGLSFVFMKASEGITSV
                     DPELSANWSGARTAGLLRGAYHFYRPGDDPTQQAEHFLATVQLGPGDLPPVLDVETAD
                     GVSADDIVQGVATWLDVVQKATGRTPIIYTSHGFWDSLGSTHLGGYPLWIAEYGVSSP
                     VVPAGWTRWTFWQYSDSGTVAGIQGAVDLDVFQGSLEELRALAEPLPH"
     gene            complement(3863..4933)
                     /locus_tag="VEL74_04170"
     CDS             complement(3863..4933)
                     /locus_tag="VEL74_04170"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYG61752.1"
                     /translation="MLSTPAIRTIESIGPYQILEDLGPAPLGTAYYAVDSRSDREVIL
                     KVIPPSRPGAGQDDTPWEILLKETEALGRIYHPGIPALDEVMEEDGALLVAFAPVEGE
                     TLHELLVGGERPDRAQLIDWGIQLLEIVAEAHRAGILHRHINEEEVVVTPDGRLVLCG
                     FGLTQLVFDPVFAMPPEQLVGEPVTERSDLYGVGLLLRRLAFTGALRGVVGGGRDPLL
                     KVLARATFPEPSTRYASAAEMAEALRDAARAARPAPVAVPACPEPDPAERKAEVAVVG
                     ERPRPISRPSPAPPDTPGEDGSADRWRTLLLLAATVLLMLLVIATGWLLIGPAGDGTE
                     AEEDATIEARPGDPPGRVAHSG"
BASE COUNT          814 a         1743 c         1790 g          751 t
ORIGIN      
        1 gagcggtgcg gggcgatcct cgacgagcgg gtgggggtga cggcggcggg ccaggcagtc
       61 cagtaggtgc agtgcgcaag gagggggcgt cctcgttgac gggggcgccc ccatcctgcg
      121 ctctctggtc ccctcccccc ctcccctgct agagttccgc ctgccatgga ccgcgaaaag
      181 gccagagaga ggatcgcccg gctccgcgcc gagatccgcc atcacgactt cctctactac
      241 gtccgagacg ccccggagat ctccgatgcg gcgtacgaca agctataccg ggagctggtc
      301 tccctcgagg agcagttccc cgacctggtg gagcccgact cccccaccca gcgggtggcg
      361 ggccagccgc tcaccagctt ccccaccgtc gagcacgccg cccccatgct ctccctggac
      421 tccgacgcct ccgaggcctc gctccgccgc ttcgacgagc ggctgcgcaa ggcgctgccg
      481 gacgtcgagg tcgagtacgt gctggagccg aagctggacg gcgcgtcggt ggagctggtc
      541 tatgaggacg gcgtcctcgc ccgcgcctcc acccgcggcg acggccggcg cggcgaaggg
      601 atcaccgaaa acgtccgcac catccaggcc gtgccgctgc ggctgcggac ggaggcaccc
      661 cacccggtgc cgccgttcct ctccctgcgc ggcgaggcca tcatgcacgc ctccgtcttc
      721 gacaagctca acgagcggct gatcgccgag ggcaaggagc cgttcgccaa tccgcgcaac
      781 gtcgccgccg gcgcgctccg ccagctcgac ccccagcaga ccgcgtccaa gccgctcgac
      841 ctcttcgcct atgacgtcct gtccgctggc gagtcggctc agattcccgg gatcgagacg
      901 cagtgggagg tgctgcaggc gctcaaggaa tggggcctgc gggtcagctc cctggtcagg
      961 aaggtgaaga cgctggagga gatcctggag taccaccagg atctcgaggc gcggcgcgac
     1021 gacctcggct tcgagatcga cggcatcgtc atcaagctga acgacctcgc ggcccgcgac
     1081 cgggtgggct ggacctcccg ccatccgcgc tgggcgttcg ccttcaagtt cccgccgcgc
     1141 aaggagatca cccgcatcct ctcgatcgtc ccgtccgtcg gccggtcggg ggtggtgacg
     1201 ccggtcgcgc tgatgctgcc ggtcgagctc ggcggcgtca ccgtctcgcg ggccaccctc
     1261 cacaaccgcg aggaggtggc gcgcaaggac gtccgcgagg gggaccgggt gcgcatccag
     1321 cgcgccggcg acgtcatccc ccaggtggtg gagcggatcg tcgagcccga gcgcgaggcc
     1381 gagcgcgaga aggagccgcg ctggcagatg ccggccacct gcccctcctg cggtacgccg
     1441 ctcatcgagc gcgggccgtt caccatctgc ccgaacggct tcgagtgccc ggcgcagctc
     1501 gtggggagga tcgtccacct cgcctcccgc gacgccctcg acatcgaggg gctgggggac
     1561 gagaccgcca agctcttcgt caacgagggg ctggtccgcc agctcccgga cgtcttcgac
     1621 ctgcgcgccg agcagctcgt gcacctcgag gggttcgccg agaagtcgtc gaagaaactg
     1681 gaggaggcgc tgcgcaaggc ctccaaggtg gagctccccc gtctcctcca cggcctgggc
     1741 atccccgagg tgggggtggc cgtggcgcgc gacctctccc gccacttcgg caccttcgag
     1801 cggatccgca cggcggacgc ggcggcgctc caggaggtgg cgggggtggg gccgcgcatg
     1861 gccgaggcga tcaccacctt cttccgagac gagaagaacg ccccgatcct ggacgagctg
     1921 ttgaagcgtc tcgacgtcca ggagcagtcc gccgcccccc cggcgtccgt actaccgctg
     1981 tcgggcaaga agttcgtctt caccggcggc cttgcccaaa tgtcccgccg cgacgcccag
     2041 aacctggtgg agtccctggg ggccaaggcc acgggatcgg tgtcgaagag caccgactac
     2101 gtggtcgtcg gggaggacgc cggctccaag gccgacgacg cccgcacgct cggggtgacg
     2161 accctggatg aagaagggtt cctggatctg ttacggagtc tgggagcggc ggtgtaagaa
     2221 gaaaggggac tgctttgaca gccaagctcg tcacaatagg gatcgtgctg gcggccttcc
     2281 tggcctggca ctggatttca ccggacaagc ccgcggcgct ggaggcggcg gagcgtcagc
     2341 agaaaggatc gaagaaggtg gacaccatcg agaagatcgt caagagcgac gccgagtgga
     2401 agaaggagat gacgtccgag gagttccacg tgatgcggga gaaggggacg gagcgggcgt
     2461 tcacgggcaa gtactggaac aaccacgaga aggggacgta cctctgcggc gcctgcggcc
     2521 tggagctgtt cagctccgag accaagttcg actcgggcac cggctggccg agcttctggc
     2581 agcccatcga tcagagccac gtggagaccg agaccgaccg cgcctggggc atgaagcggg
     2641 tcgaggtgca ctgcgcccgc tgcggcgccc acctgggcca cgtcttcgag gacggcccga
     2701 agcccacggg gctgcgctac tgcatgaact ccgtctccct cgacttcaag aaggccgagt
     2761 aagcctcgcc gcgtctctgg acggcgggcc gttccgaccg gaacagcgtc cggacggccc
     2821 cgccgttttt cacatctgac atagaatccg cgtcatcctt tccaggaggg tgagccatgt
     2881 cttccgagcc cagctcgatc cgcggcatcg atgtcagcaa atatcaggga actatcgatt
     2941 ggagccaggt cgccacggcc ggcctgtcgt tcgtcttcat gaaggcgagc gaggggatca
     3001 ccagcgtgga cccggagctc tcggcgaact ggagcggcgc tcgcacggcg gggctgctgc
     3061 gcggcgccta ccacttctat cggcccggcg acgatcccac ccaacaggcc gagcacttcc
     3121 tcgccacggt ccaactcggc ccgggggacc tgccgccggt cctcgacgtc gagaccgcgg
     3181 acggcgtgag cgccgacgac atcgtccagg gagtcgccac ctggctcgac gtggtccaga
     3241 aagcgaccgg ccgcacgccg atcatctaca cctcccatgg cttctgggac agcctgggct
     3301 ccacccatct cggcggctac ccgctctgga tcgccgagta tggagtctcg agcccggtcg
     3361 tgccggccgg atggacccgc tggaccttct ggcagtactc ggattccggc acggtcgccg
     3421 gcattcaggg ggcagtggat ctggacgtct tccaggggag cctggaggag ctgcgggcgc
     3481 tggcggagcc cctcccgcac tgatttcccc ggcgaagatt gcccatccct cgtggggcag
     3541 ccatcgcccc cggagaaatg acggtcattc ctcatggggc aggcgttgcc ccatcagaaa
     3601 cggccctcat tcctgatggg gcagcggctg ccccaacagg aacggtctcc attcctcatg
     3661 gggcagcggc tgccccggca ggaacgatct ccatttctgc tggggcagcg attgccccgg
     3721 caggaacgca caccattcct catggggcag cgactgcccc aacaggaacg acgttcattt
     3781 ctaccggggc agtggctgcc ccagcaggaa tgcgccccgt ccgtgcttcc gcggcgatgg
     3841 aggcaacgcg gatcgggggc gctcagccgg agtgggcgac gcgtccgggt ggatctcccg
     3901 gccgcgcctc gatggtggcg tcttcctcgg cctcggtgcc gtcgcctgcc ggcccgatga
     3961 ggagccagcc ggtggcgatg accagcagca tgaggaggac ggtcgcggcc aggagcagga
     4021 gggtgcgcca gcggtcggcg gagccatcct ctccgggggt gtccggcggc gcgggggacg
     4081 ggcgcgagat cggccggggc cgctccccca ccaccgcgac ctcggctttc ctctcggcgg
     4141 ggtccggctc ggggcaggcc ggaacggcga ccggcgccgg acgggcggcg cgggcggcgt
     4201 cacgcagcgc ttcggccatc tcggcggcgc tggcgtagcg cgtggacggc tcggggaagg
     4261 tggcgcgcgc cagcaccttg agcagcgggt cccggccgcc gccgacgacg ccgcggagcg
     4321 cgccggtgaa ggccagccgc cggagcagca ggcccacgcc gtagaggtcg ctacgctcgg
     4381 tgacgggctc gccgacgagt tgctcggggg gcatggcgaa gacgggatcg aacaccagtt
     4441 gggtgaggcc gaagccgcag aggaccaggc ggccgtcggg ggtcaccacc acctcttcct
     4501 cattgatgtg ccggtgcagg atgccggcgc ggtgcgcctc ggccacgatc tccaggagct
     4561 ggatccccca gtcgatgagc tgcgcccggt ccggccgctc cccccccacc agcaactcgt
     4621 gcagggtctc cccctcgacg ggcgcgaagg cgacgagcag cgcgccgtcc tcctccatca
     4681 cctcgtccag ggcgggaatg cccgggtggt aaatacggcc cagcgcctcg gtctccttga
     4741 gcaggatttc ccaaggggtg tcatcctgcc ccgctcccgg ccgcgacggc gggatcacct
     4801 tgaggatgac ctcccggtcg ctgcgcgagt cgacggcgta gtaggccgtg cccagagggg
     4861 caggccccag gtcttcgagg atctggtagg ggccgatact ctcgatggtg cggatagcgg
     4921 gtgttgatag catgggtgtc cggtcctgcc ctctataaga agatggtgca aggtccatgc
     4981 aaggggggga cggcaacgga gaatcggctg ggcggcagct cgcccggcaa cgtgcagccg
     5041 ctgtgtcgaa tgtgctcacg attcctggta gggtcgggat gtgatggaga tgtacgga
//
LOCUS       DASPJI010000161         7656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_9608, whole genome shotgun sequence.
ACCESSION   DASPJI010000161 DASPJI010000000
VERSION     DASPJI010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7656)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9608"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VEL74_04175"
     CDS             complement(<1..861)
                     /locus_tag="VEL74_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYG61753.1"
                     /translation="MLAEALAPAPEPSRPDRRFLDALRTKIEPVPLPFTYKAALAVVA
                     LAMVLLPLLYVTLIGLAAWGVAWYAMNGWALLDGIGSSRLQAASYITPLIVGGTVVLF
                     MIKPLFARRQKTDDEVPIDPAKEPLLFEFVRRTAVAVGAPVPREIRLDHEVNASASLR
                     RGLLSLFSDDLVLTIGVPLAAGMTVEQLAGVLAHEFGHFGQRAGMRLSHIVRSVSYWF
                     ARVVYERDRFDARLEHAVKESGDFRIRLVAWIAAAFVWIGRTILKGLMYVGHVISSSL
                     LRQMEFDADRY"
     gene            complement(945..1697)
                     /locus_tag="VEL74_04180"
     CDS             complement(945..1697)
                     /locus_tag="VEL74_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG61754.1"
                     /translation="MPIDVTPAAPEEVRPLRELYRQEMNCQIIRYSWYGRGWTDPYLL
                     HLNGRVVGYGLVGGIGANPRDTIIELYVLPIHRGAALPLFRRLAETSRARTVETQTND
                     VLLTLMLYDCAESIEVDKVLFEDAFTTQLTVPGAAFRRITEADAERIFVHTSEPGGEW
                     MIEAEGRVAATGGILFHYNVPYGDLFMEVAEPFQRRGYGSYLVQELKRTCYEMGRIPA
                     ARCNASNVASRAALQKAGLLPCARLLTGVLTR"
     gene            2593..3054
                     /locus_tag="VEL74_04185"
     CDS             2593..3054
                     /locus_tag="VEL74_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61755.1"
                     /translation="MEEALTIRGLTVGKLKELLAAVPDAVPIALQLEPGAAPQDLASI
                     HQLALEYNGGPLVILRPTRSEWRVQGIEEHFEITGRGLAVPLSEENWPYVIPFTAVEY
                     EIEGPAGQIRSGWAGVELLLRSGKETPALLMNSLSANEIEQGSILRFRGAA"
     gene            3041..3415
                     /locus_tag="VEL74_04190"
     CDS             3041..3415
                     /locus_tag="VEL74_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61756.1"
                     /translation="MAPRSGFHPMPFDFAEQIGALPAEQRLEFYEILAHELTISIRAF
                     WSDEALSLDEKVERMKWVNEILHRVVAKVRVFRLSLHEWTEADFAQQIQGWVEQCPAI
                     GQEVDWAIRHSYRSVTRPRADR"
     gene            3501..4133
                     /locus_tag="VEL74_04195"
     CDS             3501..4133
                     /locus_tag="VEL74_04195"
                     /inference="COORDINATES: protein motif:HMM:NF018755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HYG61757.1"
                     /translation="MRLSGFLAIGFMTWSLSAMAQQPLRPPQIAPFGDSQWWVMTEAI
                     PTRIGRSEEIITVPKGFVTDLASIPRFFWATFPKTGQYMSAAILHDYLYWDQRCTREQ
                     ADRIFDIEMKSYGVNDTARTLIFSAVSELGASAWQANGAAKAGGELRFLPEPQLAHFL
                     SQPFDATRTWPVIRSQIPRDPTRSGNSGATDPNSQLATVCARAIAANAGQ"
     gene            4234..4410
                     /locus_tag="VEL74_04200"
     CDS             4234..4410
                     /locus_tag="VEL74_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61758.1"
                     /translation="MKKNTDKKNRILGRRVARELSNEQLGPVTGAGYWTYTLLYPSDP
                     GGPRWDSTSGPIWV"
     gene            4461..5579
                     /locus_tag="VEL74_04205"
     CDS             4461..5579
                     /locus_tag="VEL74_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MvdD family ATP-grasp ribosomal peptide
                     maturase"
                     /protein_id="HYG61759.1"
                     /translation="MSRYLITTEPRDVHAVEVALALEARGAEPFLWLGADFTTLLAGS
                     IGIRDGRVGWELRGLDLPSARPPFDAVWMRRPTPPVLPASLHPADRPVAEREVRELVA
                     GLYLLAAPDAFWVNSLASRGRAESKAVQLAEAARLGFTVPPTLLSNDPERIREFLTAF
                     RGRAIYKSFYPAGWQGAETTAVLGTAEIELEELPDDEYLLASPGIFQARVEKAYEIRV
                     TAFGRYIAAAHLDSQALPQSRIDSRAAGPDIPVRPIELPPEVERRCFELMDRLGIVFG
                     CFDFAVTPEGEHVFFEVNPAGQFLWVEEACPEIRLLAPFVEFLLERRADFRWRPDPAA
                     IRHEDLAGPAFRRMEELAARHVEPPQAFIGMEDDEEPA"
     gene            complement(5576..7480)
                     /locus_tag="VEL74_04210"
     CDS             complement(5576..7480)
                     /locus_tag="VEL74_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61760.1"
                     /translation="MRALTAIFQRELMERRLLLGAALLLGLVPLLVPVLPRLGSGTLS
                     ELRGGLALALAATFGGVVALILGGSVIARDLAERRISFYFSRPLPGWTIWAGKLAAGA
                     VLALGGVALILLPVLLMGDSLSLNGNWGLDASQGAEILTVYGLLVLLLAGHASGVMLR
                     SRSPWLILDLVALLVGGWLLLDAPRLLRVVGAYKAANLLLNVILWMIPLALLVAAAVQ
                     VASGRTDLRRSHRLLSLTLWGLLLPLAFGAQGVAQWVTAVTPRHLEEIEAVIPAPAGD
                     WVYMQGEAVHRGDYTPAFLLNASNGEYVQVPPYRATPWNLRMSFSADGRRAAWTHWIP
                     GGPVRIDTVDLTRPEAGMVQSPITFYRYIEDFALSPDGGRLATMSQGRLQVDEFPSGK
                     MLASVRLPWEKVESARLHFGEDVIRLQALRIENAPSRVPTRYRDHTLYIYEYDLRSKE
                     LAKTGELSFNFNSLAWDLANHDDPSRLVLRNRETSSIQIHDARTGELRLALPVPPQSL
                     VHSALLTGDRLALAVQRRGANQLLVLAGDGTPLRRMRFRGPIHLGGQPTPETLVIASG
                     SPERPGVSWLLDLGSGQVKRIGDGLMPLIRVGLQPGSTGARLFAAKGGRLVWIDPLSG
                     QQRVVLPGHG"
BASE COUNT         1325 a         2499 c         2628 g         1204 t
ORIGIN      
        1 gtagcggtcg gcgtcgaact ccatctggcg gaggagcgag gagctgatca cgtgccccac
       61 gtacatcagc cccttgagaa tcgtgcggcc gatccagacg aaggccgccg cgatccaggc
      121 gaccaaccgg atccggaagt ccccgctctc cttgaccgcg tgctccagac gggcgtcgaa
      181 gcggtcgcgc tcgtagacca cccgggcgaa ccagtagctc actgatcgga cgatgtggct
      241 gagccgcatg ccggcgcgct gcccgaagtg gccgaactcg tgggcgagca cgccggcaag
      301 ctgctccacg gtcatcccgg ccgccagggg tacgccgatg gtgagcacca ggtcgtcgga
      361 gaacagcgac aagaggccgc ggcggagcga ggcggacgcg ttgacctcgt ggtcgagccg
      421 gatctcgcgc ggcaccggcg ctcccaccgc gaccgccgtc cgccgcacga attcgaagag
      481 cagcggctcc ttcgcggggt cgatggggac ctcgtcgtcc gtcttctggc ggcgggcgaa
      541 cagcggcttg atcatgaaca gcaccaccgt ccccccgacg atcagcggcg tgatgtacga
      601 cgcggcctgg agccgcgagg acccgatgcc gtccaggagc gcccagccgt tcatggcgta
      661 ccaggcgacc ccccaggcgg ccaggccgat gagcgtcacg tagaggagcg gcaggaggac
      721 catggcgagc gccaccaccg ccagagccgc cttgtaggtg aacggcaggg ggacgggctc
      781 gatcttcgtc cgcaacgcgt cgagaaagcg ccggtcgggc cgggatggct cgggggccgg
      841 ggcgagagct tctgcgagca tgaggctcct ccagggagag gcaggttcct ccggagtgtc
      901 ggcagccggc agaacgtttc accggcggcg cggctggggg agggtcagcg cgtcaggacg
      961 ccggtgagga gccgggcgca ggggagcagc ccggccttct gcagcgccgc ccgggaagcc
     1021 acgttggacg cgttgcaccg cgccgccggg atccttccca tctcgtagca ggtccgcttg
     1081 agctcctgca ccagatagct cccgtagccg cgccgctgga agggctcggc cacctccatg
     1141 aagaggtcgc cgtacgggac gttgtaatgg aacaggattc cgccggtcgc ggcgacccgc
     1201 ccctccgcct cgatcatcca ttcaccgccg ggctccgacg tgtggacgaa gatccgctcg
     1261 gcgtccgctt cggtgatccg acggaacgcg gcccccggca cggtgagctg ggtggtgaac
     1321 gcgtcctcga agaggacctt gtcgacctcg atcgattccg cgcagtcgta gagcatcagg
     1381 gtgaggagga cgtcgttcgt ctgcgtctcg accgtccgcg cccggctggt ttcggcgagc
     1441 cgccggaaca gcggcagggc cgcgccacgg tggatgggca ggacatagag ctcgatgatg
     1501 gtgtcccgcg gattggcccc tatgcctccg acgaggccgt atcccacgac tcggccgttc
     1561 agatggagca gataggggtc ggtccagccg cgcccgtacc aggagtagcg gatgatctgg
     1621 caattcatct cctgccggta cagctcccgc aggggccgga cctcctccgg ggcggcgggc
     1681 gtgacgtcga tcggcatgcg cctgagtcta tcgcgggcaa gacatgtcca aaggcggcgc
     1741 cgccgggccc cggtgccgct aggaacacgt acgtcgtttg tcaaagagcg aaggggaacc
     1801 ggtttcggcc ggattgtccc ctttcagggg atgaagtgta ccacggaatc cattgggatg
     1861 actccgttga cgcagggaag acttcgacgt agtctcgcgt ggaggggcgc gttggttgaa
     1921 ggacaggcgc tcgatgttcc ttgacaactg aatcgtgagg gggccgccgc cgtgtccaag
     1981 gccgctcccc cgaggactcc tccgtccgac aggaggacaa cgggaggcgg aggtccgatc
     2041 cctccgcctc ccgtgccgtt tggattccgg aacgctgggt cactacggga acctcctgga
     2101 acacaaccga acgctcctgg aacgcaacgg cgcctcgcgg gatcgctatc ccctggcgcg
     2161 cgtcattacc tcggcgttcg ggatcactcc cgcgggtggg gagtgttccc ggcgagtgag
     2221 ggaatgatcc cgccgtgctc cggagtgatc ctgccgcctc gatttgtgtt ccggaacgcg
     2281 gggagggtga ttcccccggc ttcccgggaa gtgaccccgc tatgcgatag cgccaacgtc
     2341 ctctacgagc cacgagcagg gtgctgtatc agttcaggca actgccagtg tgcatgccca
     2401 gcagacctcg attgagagac caagtggatg agagggttcc gaagagcgag cctggaagcg
     2461 tgtaacccgg cggcttgagt ggagtgctga cgcagtcgct cagctgcagg gctcgctagc
     2521 cgccaaagga gtatagatgt atgttactcg ccaatgatgg cacctgacat gaacccatga
     2581 gaggctgaga acgtggaaga ggctctgaca atccgaggcc tcacggttgg aaagctcaag
     2641 gagctgctgg cggccgtgcc ggacgcggtg cccattgctc tgcagctcga gcctggcgcg
     2701 gctcctcagg acctggcgag tattcatcag cttgcgctcg aatacaacgg cgggccgctc
     2761 gtgatcctcc ggccaacacg gtctgagtgg cgcgtccaag ggatcgagga acacttcgag
     2821 atcaccggcc gcggtcttgc tgttcctctg tcggaggaga actggccgta cgtgatacct
     2881 ttcacggccg tcgaatacga gatcgaaggg ccggcaggac agatccggtc cggctgggcc
     2941 ggtgtggagt tgcttctccg aagcggaaag gaaacgccgg ccctcctcat gaattcgctg
     3001 agtgccaacg agatcgagca gggatcgatc ctgcggttcc gtggcgccgc gtagcggttt
     3061 tcatcctatg ccattcgatt tcgcggaaca gatcggtgcc ctgcccgccg agcagcgcct
     3121 cgagttctat gagattctcg ctcacgagct caccatcagc atccgggctt tctggtctga
     3181 tgaggccctc tccctggatg agaaggtcga gcggatgaaa tgggtgaacg agatccttca
     3241 tcgggtggtc gccaaagttc gggtcttccg actgagcctg catgaatgga ccgaagcgga
     3301 cttcgcccag cagatccagg gttgggtcga gcagtgcccc gcgatcggcc aagaggtcga
     3361 ctgggccata cggcacagct accggagcgt cacccggccc cgggctgaca ggtgacgccc
     3421 gctttgacag ccctgggggg ccagactaga cttgttccgg tgtcgaggtg tgaggtcagg
     3481 ccttcatggg agagacaacg atgcgcctta gcggattcct cgcgattgga ttcatgacat
     3541 ggtcactcag cgcgatggct caacagccgc tgcgcccgcc acagatcgca ccgttcggag
     3601 attcccaatg gtgggtgatg acggaggcga ttcccacacg catcgggcga tccgaagaga
     3661 tcatcacggt acccaagggc ttcgtgaccg acctggccag catcccgagg ttcttctggg
     3721 caacgttccc gaagaccggc cagtacatgt cggcggcgat cctccatgat tatctgtatt
     3781 gggaccaacg ctgcacccgc gagcaggcgg acaggatctt cgacatcgaa atgaaatcct
     3841 atggggtcaa cgacacggcg cggacgttga tcttttccgc cgtgagcgag ctgggcgcct
     3901 cggcctggca ggcgaacgga gcagccaagg ccgggggcga gctcagattc ctgccggagc
     3961 ctcagctcgc tcatttcctt tcgcaaccct tcgatgcgac caggacgtgg cccgtcattc
     4021 gaagccagat cccccgcgac ccaacgcgat ccggcaacag tggcgctacg gatccgaact
     4081 cccaactcgc cacggtttgc gcacgggcca tcgcggccaa cgcagggcag tgaaggccgg
     4141 ggccgtctct ctgtcccgct cgcccctctt cccgcacctc cagaagtaag gagcgccagt
     4201 tgcgcaggcg cttcgtctca caaggaggtg accgtgaaga agaacactga caagaagaac
     4261 cgcattctgg gccgccgcgt ggcccgtgag ctgtcgaacg agcagctcgg accggtgacc
     4321 ggcgccggct actggactta cacgctgctc tatccgtccg atccgggcgg tccgcgttgg
     4381 gacagcacca gcggtccgat ctgggtttga ggatctctcc ccccggccgg cgttccggcc
     4441 ggggagcctc tcgcgagccg atgtccaggt atctgatcac caccgagccg cgcgacgtgc
     4501 acgcggtcga ggtcgcgctg gcgctcgagg cgcgcggcgc cgagccgttc ctctggctcg
     4561 gcgccgactt cacgactctt ctcgcgggat cgatcgggat tcgcgatgga cgcgtcggct
     4621 gggagcttcg cggcctcgat ctgccgtcgg cccggccgcc gttcgacgcg gtctggatgc
     4681 gccggccgac gccgccggtg ctccccgcga gcctccatcc cgccgaccgc ccggtggccg
     4741 agcgcgaggt gcgcgagctg gtggccggcc tctacctgct ggccgccccc gacgcgttct
     4801 gggtcaactc gctggcgagc cgcggccgcg ccgaatccaa ggcggtgcag ctcgccgagg
     4861 cggcccgcct cggcttcacc gttccgccga cgctgctgtc gaacgatccg gagcgcatcc
     4921 gcgagttcct gacggccttc cggggccggg cgatctacaa atcgttctat cccgccggct
     4981 ggcagggggc ggagaccacg gctgtgctcg gcaccgcgga gatcgagctc gaggagctgc
     5041 cggacgatga gtacctgctc gcgtcacccg gcatcttcca ggcccgggtg gagaaggcct
     5101 acgagatccg cgtgaccgcg ttcggccgct atatcgccgc cgcgcatctc gactcgcagg
     5161 cgcttccgca atcaaggatt gactcgcgcg cagccgggcc ggacatcccc gtccggccca
     5221 tcgagctgcc gcccgaggtc gagcgccgct gcttcgagct gatggaccgc ctcggcatcg
     5281 tcttcggctg cttcgacttc gccgtcaccc cggaaggtga gcacgtcttc ttcgaggtca
     5341 atccggccgg ccagttcctc tgggtggagg aggcctgtcc ggagatccgc ctgctcgccc
     5401 cgttcgtcga gttcctgctg gagcgccgcg ccgatttccg ctggcgcccc gatccggcgg
     5461 ccatccgcca cgaggacctc gccggccccg ccttccggag gatggaggag ctcgccgccc
     5521 gccacgtgga gccgccccag gcgttcatcg gcatggagga cgacgaagag ccggcctagc
     5581 catgccccgg cagcaccacc cgctgctgcc cggagagcgg gtcgatccag acgagccggc
     5641 cccctttcgc agcaaagagc ctggccccgg tgctccccgg ctgcaggcct accctgatga
     5701 gcggcatgag gccgtctcca atgcgcttca cctggcccga gccgagatcg agcagccagg
     5761 agactcccgg tctctcggga gagcccgacg caatcaccag agtttcagga gtgggttggc
     5821 ctcccaggtg gatggggccg cggaaccgca tgcggcggag aggggtgcca tctcccgcca
     5881 gcacgagcaa ttgattggcg ccgcgccgct gaaccgccag tgcgaggcgg tccccggtca
     5941 ggagggcgct atggaccaga ctctgcggag ggacgggtag ggccagcctc agctcacccg
     6001 tgcgggcgtc gtggatctgg atcgaagacg tctcacggtt gcgcagcacc agacggctgg
     6061 ggtcgtcatg gttggcgagg tcccaggcga ggctgttgaa gttgaagctg agctctcccg
     6121 tcttcgccag ctccttggac ctcaggtcgt actcgtagat gtagagggta tggtcccgat
     6181 accgggtcgg gacgcgggag ggtgcattct cgatccgcag ggcctggagc cggatcacgt
     6241 cctcgccgaa gtgaaggcgg gccgactcca ccttctccca tggaaggcgg acggatgcga
     6301 gcattttgcc cgagggaaac tcgtccacct ggagacgccc ctgggacatc gtggccaggc
     6361 gcccgccgtc cggggagagg gcgaagtcct cgatgtacct gtagaaggtg atgggcgact
     6421 ggaccatgcc ggcttcgggg cgagtcaggt ccaccgtgtc gatccgcacc gggccaccgg
     6481 gaatccaatg ggtccaggcc gcccgcctgc cgtcggcgga gaagctcatc cggagattcc
     6541 aaggcgtcgc acgatagggg gggacctgga cgtactcgcc attggaggca ttgagcagga
     6601 aagcgggagt gtagtctccc cggtgaacgg cctccccctg catgtagacc cagtctccgg
     6661 ccggcgcagg gatgacggct tcgatttcct ccaggtgccg cggagtcacg gcggtcaccc
     6721 actgggcgac accctgggca ccgaaggcga gggggagcag gagcccccag agagtgaggg
     6781 agagcagccg gtgggagcgc cggagatcgg tccggccgct cgccacctgc acggcggccg
     6841 cgacgagcag ggccaagggg atcatccaga ggatgacgtt gaggaggaga ttggcggcct
     6901 tgtacgcccc gacgactctg aggagacgcg gggcatcgag gagcagccat cctcctacca
     6961 ggagggcgac gagatccagg atgagccagg gagagcgcga gcgcagcatc accccggacg
     7021 cgtggccggc gaggagcagg accagcaggc catagacggt gaggatctcg gccccttgag
     7081 aggcgtcgag accccagttg ccgttgaggc tcagcgagtc ccccatcagc aggacgggca
     7141 ggaggatcag tgctactccg cccagagcca gcaccgcccc ggcggcgagc ttgccggccc
     7201 agatcgtcca gccggggagc gggcgcgaga agtagaagga gatacgcctc tccgccaggt
     7261 ctcgggcgat caccgagccg cccaggatca gcgcgacgac gccgccgaac gtggcggcga
     7321 gggcgagcgc caggccgccg cgcagctcgg acagggtgcc ggacccgagg cgggggagga
     7381 ccggcacgag cagcggcacc aggcccagga gaagagcggc gcccagcagc aggcgccgct
     7441 ccatgagctc gcgctggaag atcgcggtca gagctctcac ggaggacatc agcggccacc
     7501 tttcgtttcc ccgaccaggg agaggaagat ctcttcgagg gtgagcgacg agacctcgaa
     7561 gcggcgcccg tcgcggcgct ggaaatgatc gaactgtcct tcgtcgtact tcgagaccac
     7621 ggcctcgacc gcccaaccgc gggccgctac tgccac
//
LOCUS       DASPJI010000162        10521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_9618, whole genome shotgun sequence.
ACCESSION   DASPJI010000162 DASPJI010000000
VERSION     DASPJI010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10521)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10521
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9618"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VEL74_04215"
     CDS             complement(<1..545)
                     /locus_tag="VEL74_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61761.1"
                     /translation="MSINSLHPLPQAQLVERAKALEWLLLDVDGVFTDGRLIYGADGE
                     QWKVFDVHDGLGLRFLQRGGVKVGILSGRMNEALEFRAREIDLDTVIMDRSDKGPAFA
                     EFLDSHGVDPRKVAYMGDDLLDLPVMRLCGLSFAPVDAVFEVRQRVDQVLERRGGHGV
                     VRELCELLLRARGQWDELIAPL"
     gene            complement(545..1531)
                     /locus_tag="VEL74_04220"
     CDS             complement(545..1531)
                     /locus_tag="VEL74_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HYG61762.1"
                     /translation="MSVAPVALPRSPRTVAAEVLETEAAAIRGLLPQLDERFDRAVEL
                     LRGCTGRVVCSGMGKSGLIMKKVAATLSSTGTPSLFLHPAEAVHGDLGMIVEGDVVLA
                     ASYSGTTEELLKLVETLKRLGVPLVVMTANAASPLARHADIHLPVAIDREACPLNLAP
                     TASTTATLALGDALAMALLEARGFTREDFARLHPAGQLGKRLLRVDQLMHSGEALPKV
                     TLDASMREAIYEMSKKGLGITAVVDTGGNLAGCISDGDLRRLLAADEGLLRRTAGECM
                     RPGPRTIPGSELASAALNAMEDNRITSLFVCGESGRLEGVVHLHDLWGLELF"
     gene            complement(1528..2382)
                     /gene="kdsA"
                     /locus_tag="VEL74_04225"
     CDS             complement(1528..2382)
                     /gene="kdsA"
                     /locus_tag="VEL74_04225"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HYG61763.1"
                     /translation="MAVDPVVLAPGVVVGGSGGTLPVIAGPCVIESEDRTLEAAHFLA
                     AMAERLRLPLVFKSSFDKANRSSIKSFRGPGLAEGLRVLSLVKAETGLPVLTDVHEPE
                     QCGPAAEVCDVLQIPAFLCRQTDLVVAAASTGRAVNVKKGQFMAPDDMRRVVDKARST
                     GNDRVTVTERGVSFGYHNLVVDMRSFAMLHEDGIAVIYDVTHSLQLPGAAGEESGGLR
                     RYAKPLARAAVAAGADGIFMEVHPDPDQALSDRAVQLDPVRAESLLRSLQELRRVVAG
                     GVMEEVAR"
     gene            complement(2382..4091)
                     /locus_tag="VEL74_04230"
     CDS             complement(2382..4091)
                     /locus_tag="VEL74_04230"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HYG61764.1"
                     /translation="MASKYIFITGGVVSSLGKGVAASSVGAILEARGFSVTLMKFDPY
                     INVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTHTTTSKRHNFTTGKIYETVINK
                     ERRGDYLGKTVQVIPHITDEIKEAMRRVGDDNDVVIVEIGGTVGDIESLPFLEAIRQF
                     RQELGRGNAINIHLTLVPFIAAADELKTKPTQHSVRELREIGIQADVVMCRVDRALPE
                     DMKRKIALFCNVDTRQVIAARDVSTIYEVPLTLCREGLDDIVLELLNLPHYDRDMSRW
                     ESLVARVKNPHNRVRVGIVGKYVEFPDSYKSLNEALIHGGIANDAKVELVYIDAEELE
                     GHNWPSEMSEVDSLLVPIGFGPRGTEGKIRAIRYAREHKVPFFGICLGMQCTVIEHAR
                     NVCGLKNANSSEFDAETEHAVIYKLRDLLGIEELGGTMRLGAYPCRLKEGTLARKIYG
                     TAEISERHRHRYEVNQKYMQTLIDHGLVISGLSPDGKFVEMVELPDHPWFLGCQFHPE
                     YKSKPTEPHPLFVSYIAAALAHQQRRREGDAAKAGGDGQGVITLPGELAEVPVASEMR
                     AEG"
     gene            complement(4115..4879)
                     /gene="kdsB"
                     /locus_tag="VEL74_04235"
     CDS             complement(4115..4879)
                     /gene="kdsB"
                     /locus_tag="VEL74_04235"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HYG61765.1"
                     /translation="MHPSAGPPPNPWMIGSGIVGAIPARYGSSRLPGKPLLPIAGRPM
                     IEHVYERVARARGLDRVVVLTDDERIARAVQAFGGNCEMTPEDCASGTDRIAWAARGW
                     QAEAVVNIQGDEPLIDPEVVSRVAEHLRVHPEDPVVTLAAPLDPAERDNPNAVKVVLS
                     LAGYALYFSRSPIPYERQQGIAQPLKHLGLYGYRGDALQRLAALEPTPLERAESLEQL
                     RALENGIPLRVLLVEAASPGVDTREDLERVERLLGA"
     gene            5012..6091
                     /locus_tag="VEL74_04240"
     CDS             5012..6091
                     /locus_tag="VEL74_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61766.1"
                     /translation="MLLAMALVVAVVLALALANRAAHSFGAGAFLPTGKAQWIWSGRM
                     RHSQEPGAFYAVRDFAIDRAPDRARLLVMGDEEYVLYLNGRRVGSGGYRNGAGIDAYE
                     VGPLLLPGGNRLMAELRSSGGAGGFLLSLGDETGRPIVVTDESWRVLDRDHPGLMRGW
                     LPVGYEGLGPVGEPAFCWGVPPVGRWGSPEPGTARPLFAELAPAEIPGVRRLRPGGQD
                     LPGGARVVYDWGREVTGYLSLELRPGDQLQTALLYTGDAPPRPGIDRPAGAVLLLPGR
                     RQWLDATPRRFRYAMVIGIDEPLAARVHALAPDLTPEKVTRLLPKEGQSGVLGIEPPP
                     LRTPVEDEVWRKLESVPGIAGRKDL"
     gene            complement(6014..7039)
                     /locus_tag="VEL74_04245"
     CDS             complement(6014..7039)
                     /locus_tag="VEL74_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYG61767.1"
                     /translation="MQNQPLVDAQQSSNHRSSLASRFMELVADKLVATEAVFRHHLDA
                     DVPFIHQAGDYLSNGGGKRVRPALLLLSSRLLGHDGDEEVTYAAVVELIHTATLIHDD
                     VIDHAGLRRGQATLNQLWGNNLTVLLGDWIYTKAMQMALSHGQVEVIQRLCGATLKMT
                     EGELLAFQRLGAIDLTTEEYFEIIDRKTAHLFAASCAIPALIPPRRSDAEQVLTRYGR
                     ALGTCFQLVDDLLDFTAREAELGKPVLSDLKEGKLTLPLIILLPRVEAAERRRIEMVL
                     EDRSFSRVAPEEILDLVQSEGTLEEVAEMAVDFAARAKRELEIFPSCDARDALEFAPD
                     FVLHRRS"
     gene            complement(7080..7760)
                     /locus_tag="VEL74_04250"
     CDS             complement(7080..7760)
                     /locus_tag="VEL74_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61768.1"
                     /translation="MAPFRRSGRPQGPARVIPSLAVIALLLLSAAVPALAARYAQGER
                     IQVTGIISDRQGKPLANLQVALEVSRNTFSFRELRRTPKDIRRVSAVTNAQGEYTLVW
                     PWDSYFNQFDLVVGVPVRKSQGERLEELARVDITQRMLAGNPVVATATVENVRFLNNL
                     RAFVASVQSEDERRVYEEMGKPDEVKRVQYPDHVEASWWYFEAGKAYRFRDGRLEQVV
                     PFDPVTGR"
     gene            complement(7775..8272)
                     /locus_tag="VEL74_04255"
     CDS             complement(7775..8272)
                     /locus_tag="VEL74_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HYG61769.1"
                     /translation="MAADNSFDIVSTVDLQEVRNAVVQAEKEISTRYDLKKASCELAL
                     EGEEIVAGAADEFSLTQVLEVLKSKLVRRNVNLKSLRYGKVEPASGGRVRQKITLQQG
                     IPQETAKKLVAEIKARKLKVQAAIQGDTVRVSGKNRDDLQTVITTLKALDLDVPLTFT
                     NYRSL"
     gene            complement(8389..9039)
                     /locus_tag="VEL74_04260"
     CDS             complement(8389..9039)
                     /locus_tag="VEL74_04260"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HYG61770.1"
                     /translation="MRPKTLVPFALALALVAVAAIDVSSAQDAADPWKTLQGVRNSLV
                     EAGATGAAFTQTYVPAGFTSGEQESGRLALNLPDCLRWDYQQPYPKSFLLCGGVAHSW
                     NPEDKTGRRYRVDRKNEPGLDLLLLGVEDLKRRYNATSRPADGGRIQISLSPKQKVDA
                     LADAVLTVDPKSERLVEVSYHDREGNLTRFQINGYRSLTGQAAQFSPPRDIRWDES"
     gene            complement(9133..9504)
                     /locus_tag="VEL74_04265"
     CDS             complement(9133..9504)
                     /locus_tag="VEL74_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61771.1"
                     /translation="MRRTRLSIALALTVLVGGAALAPAEEVEETVPAEAQPDTQASPQ
                     RPLLPEELQQVLTPQAEPKAPVLYFAGRHPCSVSIPCESDSSVSCSTTGMGQSCYYRW
                     DYSPTYPGYVKCGNTVVSCAP"
     gene            complement(9577..10080)
                     /locus_tag="VEL74_04270"
     CDS             complement(9577..10080)
                     /locus_tag="VEL74_04270"
                     /inference="COORDINATES: protein motif:HMM:NF022526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2780 domain-containing protein"
                     /protein_id="HYG61772.1"
                     /translation="MDDIIKTLAGQLGLSTEQAAGGAGAVINMIRDGIDANDFKKLAT
                     MVPEISGWMKAAPTAGDAGAGGGGILGQAGDLLGSLAGGAGGGAAGGVLGSLGGLAGL
                     VGALGKLGIDPETAAKFVPTLLQFLQSKAGADLVAKILANVPALKNLGGAGGGGDLAG
                     MLGGLFR"
     gene            10169..>10521
                     /locus_tag="VEL74_04275"
     CDS             10169..>10521
                     /locus_tag="VEL74_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61773.1"
                     /translation="MSPLDMEIRIHLWSVPETRQERALVAGRVFPELRRRAEAREIGV
                     SYVGHEPTACDILVALVSDDRPPFPPSPSRIDLVYSRGEDPDLLAQRVFEDLWTAVTH
                     ERRREIFQPPAPVPP"
BASE COUNT         1476 a         3620 c         3787 g         1638 t
ORIGIN      
        1 aggggggcga tcagctcgtc ccactggcca cgggcgcgga gcagcagctc gcacagctcc
       61 cgcaccaccc cgtggccgcc ccggcgctcc agcacctggt ccactcgctg ccgcacctcg
      121 aagaccgcgt cgaccggcgc gaaggagagc ccgcagagcc gcatcaccgg caggtccagc
      181 aggtcgtccc ccatgtaggc caccttccgg ggatcgacgc cgtggctgtc caggaactcg
      241 gcgaaggccg gccccttgtc cgagcggtcc atgatgacgg tgtcgaggtc gatctcgcgc
      301 gcgcggaact ccagggcctc gttcatccgc ccggagagga tccctacctt gacccctccg
      361 cgctgcagga agcgcaggcc gaggccgtcg tgcacgtcga agaccttcca ctgctcgccg
      421 tcggcaccgt agatcaggcg cccgtcggtg aagacgccgt cgacgtccag gagcagccat
      481 tcgagggcct tggcccgctc gacgagctgg gcctggggga gaggatggag cgagttgatc
      541 gacatcagaa gagctccaaa ccccagaggt cgtggaggtg gaccacccct tcgagccggc
      601 cggactcgcc gcagacgaag agggaggtga tgcggttgtc ctccatggcg ttcagcgccg
      661 cggaggcgag ctcggagccg gggatggtgc gcggcccggg tcgcatgcac tcgccggccg
      721 tgcgccgcag gagcccctcg tccgccgcca ggagccgccg caggtcgccg tctgagatgc
      781 agccggcgag gttcccgccg gtatccacca cggcggtgat ccccagcccc ttcttggaca
      841 tctcgtagat ggcctcgcgc atggaggcgt ccagggtcac cttggggagc gcttcgccgg
      901 agtgcatgag ctggtccacc cgcagcagcc gttttccgag ctgccccgcc gggtggaggc
      961 gggcgaagtc ctcgcgggtg aagccgcggg cctcgaggag cgccatggcc agggcgtccc
     1021 ccagggcgag cgtcgccgtg gtggaggccg tgggcgccag gttcagcggg caggcctcgc
     1081 ggtcgatcgc caccggcagg tggatatcgg cgtgacgggc caggggcgag gccgcgttgg
     1141 cggtcatcac caccagcggc acgcccaggc gcttgagcgt ctccaccagc ttgagcagct
     1201 cttcggtggt ccccgagtag gaggcggcga ggaccacgtc cccctcgacg atcattccca
     1261 gatcgccgtg gaccgcctcg gccgggtgga ggaagaggga gggggtgccg gtggaggaga
     1321 gcgtggccgc caccttcttc atgatcaggc cgctcttgcc catgcccgag cagaccaccc
     1381 ggccggtgca gccgcgcagc agctcgaccg cccggtcgaa gcgctcgtcg agctgcggca
     1441 gcagcccgcg gatcgccgcc gcctccgtct ccagcacctc cgccgccacg gtgcgcgggg
     1501 agcgggggag ggcgacagga gccacgctca ccgcgccacc tcttccatca ccccgcccgc
     1561 caccacccgc cgcagctcct ggagagagcg cagcaggctc tcggcgcgga ccggatcgag
     1621 ctggacggcg cggtcggaga gcgcctggtc ggggtcgggg tggacctcca tgaagatgcc
     1681 gtcggcgccg gcggccacgg cggcgcgggc cagcggcttg gcatagcgcc gcaggccgcc
     1741 gctctcctcg ccggcggcgc cggggagctg cagcgagtgg gtgacgtcgt agatcaccgc
     1801 gataccgtcc tcgtgaagca tggcgaagct gcgcatgtcc accaccaggt tgtggtagcc
     1861 gaaggagact ccgcgctcgg tgacggtcac ccggtcgttg ccggtgctcc gcgccttgtc
     1921 caccacccgg cgcatgtcgt ccggagccat gaactgcccc ttcttgacgt tgaccgcccg
     1981 gccggtcgac gccgcggcca ccaccaggtc ggtctgccgg cagaggaagg cggggatctg
     2041 gaggacgtcg cagacctcgg ccgccgggcc gcactgctcc ggctcgtgga cgtcggtgag
     2101 caccggcagc ccggtctcgg ccttgaccag ggacagcacg cgcaatccct cggccaggcc
     2161 ggggccgcgg aagctcttga tcgagctgcg gttcgccttg tcgaacgacg acttgaagac
     2221 caggggcagg cggagccgct cggccatggc cgcgaggaag tgggccgcct ccagcgtccg
     2281 gtcctcactc tcgatgacgc aggggccggc gatcaccggg agcgtgccgc cgctcccgcc
     2341 gaccaccacg cccggggcca gcaccacggg atcgaccgcc atcacccctc cgcccgcatc
     2401 tcggaggcga ccggcacctc cgccagctcc ccgggcaggg tgatcacgcc ctggccatca
     2461 ccccccgcct tggccgcgtc cccctcccgg cgccgctgct ggtgtgccag cgccgccgcg
     2521 atataggaga cgaacagcgg atgcggctcg gtcggcttcg acttgtactc gggatggaac
     2581 tggcagccca ggaaccacgg gtggtccggc agctccacca tctcgacgaa cttgccgtcc
     2641 ggcgacaggc ccgagatgac cagcccgtgg tcgatcaggg tctgcatgta cttctggttc
     2701 acctcgtagc ggtggcggtg gcgctcgctg atctcggccg tgccgtagat cttgcgggcg
     2761 agggtcccct ccttgagccg gcaggggtag gcgcccagcc gcatggtgcc gccgagctcc
     2821 tcgatcccca gcaggtcgcg cagcttgtag atgacggcgt gctccgtctc cgcgtcgaac
     2881 tccgaggagt tggcgttctt gaggccgcag acgttcctgg cgtgctcgat gaccgtgcac
     2941 tgcatcccca ggcagatgcc gaagaagggg accttgtgct cgcgggcgta gcggatggcg
     3001 cggatcttcc cctcggtgcc gcgggggccg aagccgatgg gtaccagcag cgagtccacc
     3061 tcggacatct cgctcggcca gttgtgcccc tccaactcct cggcgtcgat gtagaccagc
     3121 tccaccttgg cgtcgttggc gatgccgccg tggatcagcg cctcgttcag gctcttgtag
     3181 gagtcgggga actcgacgta cttgccgacg atccccaccc gcacccggtt gtgcgggttc
     3241 ttgacccgcg ccaccagact ctcccagcgc gacatgtcgc ggtcgtagtg gggtaggttg
     3301 agcagctcga ggacgatgtc gtccagcccc tcgcggcaga gggtgagggg gacctcgtag
     3361 atggtcgaga cgtcccgggc ggcgatgacc tggcgggtgt cgacgttgca gaagagagcg
     3421 atcttcctct tcatgtcctc gggcagggcg cggtcgaccc ggcacatgac gacgtcggcc
     3481 tggatgccga tctcccgcag ctcgcgcacc gagtgctggg tcggcttggt cttcagctcg
     3541 tcggcggcgg cgatgaaggg caccagggtg aggtggatgt tgatcgcgtt gccccggccc
     3601 agctcctgcc ggaactggcg gatggcctcg aggaacggca gcgactcgat gtcgcccacc
     3661 gtgccgccga tctcgacgat caccacgtcg ttgtcgtcgc ccacccggcg catcgcctcc
     3721 ttgatctcgt cggtgatgtg ggggatgacc tggaccgttt tgcccaggta gtcgccccgg
     3781 cgctccttgt tgatcaccgt ctcgtagatc ttgccggtgg tgaagttgtg gcgcttgctg
     3841 gtggtggtgt gggtgaatcg ctcgtagtgc cccaggtcga ggtcggtctc ggcgccgtcg
     3901 tcggtgacga acacctcgcc gtgctggtag ggcgacatgg ttcccggatc gacgttgatg
     3961 taggggtcga acttcatcag cgtgaccgag aagccccggg cctcgaggat cgcccccacg
     4021 gaggaggcgg cgacgccctt gccgagcgag gagacgaccc cgccggtgat gaagatgtac
     4081 ttgctggcca tccgtccgtc tctccttggt ttcgtcacgc gccgagcaga cgctcgactc
     4141 gctccaggtc ttcccgcgtg tccaccccgg gcgaggccgc ctcgaccagg agcacgcgga
     4201 gcggaatgcc gttctccagc gcccggagct gctccagcga ttccgcgcgc tccaggggcg
     4261 tgggctccag ggcggcgagg cgctgcaggg cgtcgccgcg gtagccgtag aggcccagat
     4321 gcttgagcgg ctgggcaatc ccctgctgcc gctcgtacgg aatgggcgag cggctgaagt
     4381 acagcgcgta gccggcgagg gacagcacca ccttcacggc gttggggttg tcccgctccg
     4441 ccgggtccag gggcgccgcc agggtgacca ccgggtcctc cggatggacc cgcaggtgct
     4501 cggcgacccg cgacaccacc tccgggtcga tcagcggctc gtccccctgg atgttgacca
     4561 ccgcctcggc ttgccagccc cgcgccgccc aggcgatgcg gtcggtgccg ctggcgcagt
     4621 cctccggggt catctcgcag ttgccgccga aggcctgcac ggccctggcg atccgctcgt
     4681 cgtccgtgag caccaccacc cggtcgaggc cgcgcgcccg cgccacgcgc tcgtagacgt
     4741 gctcgatcat cgggcgcccc gcgatgggga gcaggggctt gccgggtagg cgcgacgagc
     4801 cgtaacgggc cgggatggcg ccgacgatgc ccgagccgat catccagggg ttcgggggcg
     4861 ggccggcgga ggggtgcacg acctaatcta aagcgtcgag gcggagggtg tcaacggcgg
     4921 cgtgccacga ttctcccctc cccgcccccg gtgttacagt ccccccgtgc tctcccggct
     4981 ccggaaatcg ctctcgcccc acgggcgccg catgctcctg gccatggccc tggtggtggc
     5041 ggtggtgctc gccctcgccc tggcgaaccg ggccgcccac tcgttcggcg ccggcgcctt
     5101 cctgccgacc ggcaaggcgc agtggatctg gtccggacgg atgcgccact cccaggagcc
     5161 cggcgcgttc tatgcggtcc gcgatttcgc gatcgaccgg gcgccggacc gggcgcgcct
     5221 gctggtgatg ggggatgaag agtacgtcct ctatctcaac ggccgccggg tcggctccgg
     5281 cggctaccgc aacggcgcgg ggatcgacgc ctacgaggtc gggccgctgc tcctgccggg
     5341 cggcaaccgg ctgatggcag agctgcggag cagcggcggc gccggcggtt tcctcctcag
     5401 cctcggggac gagacgggcc gtccgatcgt ggtcaccgac gagagctggc gcgtcctgga
     5461 ccgcgaccac ccgggcctga tgcggggttg gctcccggtc ggctatgagg ggctgggacc
     5521 ggtgggggag ccggccttct gctggggcgt gccccccgtc ggccggtggg ggagccccga
     5581 gcccggaacg gcaaggcccc tcttcgccga gctggcgccc gccgagatcc cgggggtgcg
     5641 ccggctccgg ccgggcggcc aggacctccc cggcggcgcg cgggtggtct acgactgggg
     5701 acgcgaagtg accggctacc tctccctcga gctgcggccc ggggaccagc tccagaccgc
     5761 cctgctctac accggcgacg cgccgccgcg gccggggatc gaccggcccg ccggcgccgt
     5821 cctcctgctg ccgggccggc ggcagtggct ggacgccacg ccccgtcgtt tccgctatgc
     5881 catggtgatc gggatcgacg agccgctcgc cgcccgggtc cacgcgctgg ccccggacct
     5941 gacgccggag aaggtgactc ggctgctgcc gaaggagggg cagagcggag tgctggggat
     6001 cgagccgccg ccgctacgaa cgccggtgga ggacgaagtc tggcgcaaac tcgagagcgt
     6061 cccgggcatc gcaggacgga aagatctcta gctcccgctt cgcccgggcg gcgaagtcga
     6121 ccgccatctc cgccacctcc tccagcgtcc cctcggactg caccaggtcc aggatctcct
     6181 ccggcgccac gcgggagaag ctccggtcct ccagcaccat ctcgatccgc cgccgctcgg
     6241 ccgcctcgac ccgcggcagg aggataatca gggggagcgt gagcttgccc tccttgaggt
     6301 ccgacagcac gggcttgccg agctcggcct cgcgcgcggt gaagtccagg aggtcgtcga
     6361 cgagttggaa gcaggtaccg agggcgcggc cgtagcgggt gaggacctgc tcggcgtcgg
     6421 agcgccgcgg cgggatgagc gccgggatcg cgcaggaggc ggcgaacagg tgcgccgtct
     6481 tgcggtcgat gatctcgaag tactcctcgg tggtgaggtc gatcgccccc aggcgctgga
     6541 acgcgaggag ctccccctcg gtcatcttga gcgtggcgcc gcagaggcgc tggatgacct
     6601 ccacctggcc gtgcgagagc gccatctgca tcgccttggt gtagatccag tcgcccagca
     6661 gcaccgtcag gttgttgccc cagagctggt tgagcgtcgc ctggccgcgg cgcaggccgg
     6721 cgtggtcgat gacgtcgtca tggatcaggg tggcggtgtg gatcagctcc accacggcgg
     6781 cgtaggtgac ctcctcgtcg ccgtcgtgcc cgagcagccg ggacgacagg aggaggaggg
     6841 cgggccggac gcgcttgccg ccgccgttcg ataggtagtc ccccgcctgg tggatgaacg
     6901 gcacgtcggc gtccaggtgg tgccggaaca ccgcctccgt ggcgaccagc ttgtccgcca
     6961 ccagctccat gaagcggctg gccagggagg aacggtggtt gctgctctgc tgcgcgtcga
     7021 cgagcggttg gttctgcacc aggtcgggcc tcatgggagg gcggttggcg gccacgccgt
     7081 cagcgccccg tcaccgggtc gaaggggacg acctgttcca gccggccgtc gcggaagcgg
     7141 tacgccttgc ccgcctcgaa gtaccaccag gaggcctcga cgtggtcggg gtactggacc
     7201 cgcttgacct cgtccggctt gcccatctct tcgtagacgc ggcgctcgtc ctcgctctgc
     7261 acgctggcga cgaaggcgcg gaggttgttc aggaagcgga cgttctccac cgtcgccgtg
     7321 gccaccaccg ggttgccggc gagcatccgc tgggtgatgt ccacccgcgc cagctcctcc
     7381 agccgctccc cctggctctt gcgcaccggc acgcccacca ccagatcgaa ctggttgaag
     7441 tagctgtccc agggccagac cagggtgtac tccccctgcg cgttggtgac ggcgctcacc
     7501 cgccggatgt ccttgggggt gcggcgcagc tcgcggaagc tgaacgtgtt gcgcgacacc
     7561 tcgagagcga cctggaggtt cgccagcggc ttcccctgcc ggtcggagat gatgccggtg
     7621 acctggatgc gttccccctg ggcgtagcgg gccgccagcg ccgggacggc ggcggacagc
     7681 agcaggaggg cgatgacggc gagggacggg atcacgcggg ccggcccctg gggccgcccg
     7741 ctccggcgaa acggggccat acgagggatc tctctcacag cgagcgatag ttggtgaacg
     7801 tgagtggtac gtcgaggtcc agggccttca aggtggtgat gaccgtctgc aggtcgtcgc
     7861 ggttcttgcc cgagacccgc acggtgtccc cctggatggc ggcctggacc ttgagcttgc
     7921 gcgccttgat ctcggcgacc agcttcttgg ccgtctcctg ggggatgccc tgctggagcg
     7981 tgatcttctg gcgcacccgg ccgccgctcg ccggctcgac cttgccgtag cgcagcgact
     8041 tgaggttgac gttgcggcgc accagcttgg acttgagcac ctcgagcacc tgggtgaggg
     8101 agaactcgtc ggcggcgccg gcgacgatct cctccccttc gagagcgagc tcgcagctcg
     8161 ccttcttgag gtcgtagcgg gtggagatct ctttctccgc ctgtacgacg gcattgcgga
     8221 cctcttgcag gtccacggtc gacacgatgt cgaaggaatt gtcggcagcc atggtgatct
     8281 cgttcgactc cggcggtcca tcgcagggct gacgcgggag agcctctcgg ctgggcgtca
     8341 ttatatatag aggagggggg gcgggccatc cgcccccgtc cagggcccct acgactcgtc
     8401 ccagcggatg tcccgggggg gcgagaactg ggcggcctgc ccggtgagcg accggtagcc
     8461 gttgatctgg aaccgggtga ggttgccctc ccggtcgtgg taggagacct ccaccagccg
     8521 ctcgctcttg ggatcgacgg tgaggacggc gtcggccagc gcgtccacct tctgcttcgg
     8581 cgagagggag atctggatcc gtcccccgtc ggcggggcgg ctggtcgcgt tgtagcgccg
     8641 cttcaggtcc tcgaccccca ggaggagcag gtcgagcccg ggctcgttct tgcggtccac
     8701 ccgatagcgt cgtccggttt tgtcctccgg gttccaggag tgggcgacgc cgccgcagag
     8761 gagaaaactc ttggggtagg gctgctggta gtcccaccgc aggcagtcgg ggaggttcag
     8821 ggccaagcgg ccggactcct gctcgcccga ggtgaagccg gcgggtacgt aggtctgggt
     8881 gaacgccgcg ccggtggctc ccgcctcgac gaggctgttg cgcaccccct ggagcgtctt
     8941 ccagggatcg gcggcgtcct gtgcggagga cacatcgata gctgcaacgg ccacaagggc
     9001 cagggcgagg gcgaacggaa cgagggtttt cggtctcatg gtcgggtctt cctatcgaag
     9061 tccctgcata caacaggcca ggggagggga tgcttccccg ggcggtggga gtcccgcccg
     9121 gggtggacca ggctaggggg cgcaggagac gaccgtgttg ccgcacttca cgtagccggg
     9181 gtaggtgggg gagtagtccc accggtagta gcagctctgg cccatcccgg tggtcgagca
     9241 ggagaccgag ctgtcggact cgcaggggat cgagacggag caggggtggc gtccggcgaa
     9301 atacaggacg ggggccttgg gctcggcctg cggggtcagc acctgctgca gctcctcggg
     9361 gagcaggggg cgctgcggcg aggcctgggt atccggctga gcctcggcgg gcacggtctc
     9421 ttcgacctcc tcagcggggg ccagcgcggc gcctcctacg agaacggtca gggccagggc
     9481 gatggacaga cgggttctgc gcatgtttct ctcctcctga tcaaagggga gcccgagggg
     9541 gggctccacc gggacgccgg ccgggagggt ccccggtcag cggaacagcc cacccaacat
     9601 gccggcgagg tcgccgccgc ctccagcgcc gccgaggttc ttgagcgcgg gaacgttggc
     9661 gaggatcttg gcaaccaggt cggcgcccgc cttgctctgc agaaactgga gcagggtcgg
     9721 cacgaacttg gccgcggtct ccgggtcgat gcccagcttg ccgagggcgc ctacgagacc
     9781 agccaggccg ccgagggagc cgaggacacc tcccgcggcg ccgccgcccg caccgccggc
     9841 cagggacccg aggaggtcgc cggcctggcc gaggatccca ccgccaccgg ccccggcatc
     9901 gcccgccgtg ggggcggcct tcatccagcc ggagatctcg gggaccatgg tcgcgagctt
     9961 cttgaagtcg ttcgcgtcga tcccgtcgcg gatcatgttg atgaccgcgc ccgccccgcc
    10021 cgccgcctgc tcggtgctca ggccgagttg cccggccagc gtcttgatga tgtcgtccat
    10081 gccgccctcc tctcagaagt tgcctctccg cgcgaagact acgcggaggc ggggaagggc
    10141 gcaagcgtgg ggcggttctg gagatatgat gtctcctctc gacatggaaa tccgcatcca
    10201 cctctggagc gttcccgaaa ctcgtcagga gcgcgccctc gtcgccggcc gggtcttccc
    10261 cgagctgcgg cggcgcgccg aggcgcgtga gatcggcgtg tcgtacgtcg ggcacgagcc
    10321 cacggcgtgc gacatcctgg tggcgctggt gtcagacgac cggccgccgt tccccccctc
    10381 cccctcccgg atcgacctcg tctactcgcg gggtgaggat ccggacctcc tggcccagcg
    10441 ggtcttcgaa gacctctgga cggcggtgac gcacgagagg cggcgcgaga tcttccagcc
    10501 gccggccccc gtgccgccgg a
//
LOCUS       DASPJI010000163         8796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_411219, whole genome shotgun sequence.
ACCESSION   DASPJI010000163 DASPJI010000000
VERSION     DASPJI010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8796)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8796
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411219"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VEL74_04280"
     CDS             <1..447
                     /locus_tag="VEL74_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HYG61774.1"
                     /translation="QAVIVGRSSIVGKPMAGLLLREHCTVTICHSRTRDLAAITREAD
                     ILVAAIGRAAMLGPEHVKEGAVVIDVGITRVHDRDEVERLYPGDPERRRHLEKRGSTL
                     IGDVDFTRVAPIASAITPVPGGVGPLTVAMLIANTLKAAKRRQGLS"
     gene            444..1082
                     /gene="coaE"
                     /locus_tag="VEL74_04285"
     CDS             444..1082
                     /gene="coaE"
                     /locus_tag="VEL74_04285"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HYG61775.1"
                     /translation="MTRDGRPLLVGLTGGLASGKSTVAGWLADAGFRVVDADRLVADL
                     YRPGGAGAEAVRGLFGDGMLDEQGAVVPARVADRIFSDPEARRALEGAVHPLVRERFH
                     EIARESSQGVVVLEATLLVEAGYSPSFDLIVTVEAPAVTRLRRAMERGMSEDAARARL
                     LAQGDGEVRREASHRIIDNCSDLPHLRHQVDDLVIELRRLADQQAEQRSEPR"
     gene            1079..1651
                     /locus_tag="VEL74_04290"
     CDS             1079..1651
                     /locus_tag="VEL74_04290"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HYG61776.1"
                     /translation="MSLPPFVLVTANPGKLVEARRLAGHDVESAAVDLPEIQSLDLEE
                     VLRAKAEAAWSRLGRPLVVEDSGLDLTALNGFPGPLIKWMLTAVGAEGIARAGLSLGD
                     PRATARCMLFYKDAYREVIVEGVTAGTLVLPGRGTRGFGWDPVFLPEGGELTFGELDG
                     AEKDAVSHRGKAWRAMIRRLAGGRRELKQD"
     gene            1946..2344
                     /locus_tag="VEL74_04295"
     CDS             1946..2344
                     /locus_tag="VEL74_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61777.1"
                     /translation="MPRVSSAVTISVWEALIDGVKQFADELPHLQELQAELESTLTEA
                     RRCQTAYLRYHARAMQEARRMREISEKGQRIESRIRAGLKSAHGADSPKLIRYGLRPH
                     KGMRKSAPREVAPAEPEIDEEVGETRDGEG"
     gene            complement(2341..3276)
                     /gene="argF"
                     /locus_tag="VEL74_04300"
     CDS             complement(2341..3276)
                     /gene="argF"
                     /locus_tag="VEL74_04300"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HYG61778.1"
                     /translation="MTQRHLISDHDLSAAEVAGLFKLAADVKANPEKYSGALTRKSLG
                     MIFEKSSTRTRVSFEVGMVQMGGHALFLSSRDIQLGRGEPISDTAQVLSRYVDGIMAR
                     VFAHQTVADLARFGTVPVINGLSDDLHPCQALTDYFTLTEVFGSDLKGRKLAYVGDGN
                     NMAMSLMLTASRVGMDFAVASPKGYEVREPYLGQARRAAEEAGTRLELTDDPRQAVKD
                     ASAVYTDVWASMGQEQEAQKRLKDFQGFIVDEKLMSGARQDAIFLHCLPAHRGEEVAA
                     EVADGPQSRIYDEAENRLHVQKAILLWLMAGVEIA"
     gene            complement(3356..4633)
                     /locus_tag="VEL74_04305"
     CDS             complement(3356..4633)
                     /locus_tag="VEL74_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HYG61779.1"
                     /translation="MPIHVETEIGRLRRVLVHRPGREIDWMVPALMEHLLFDDILYGE
                     EAREEHDAFTGVLRQAGVQVHYAHDLLADVLREETVRRKLLDELEAEYGVPFSVAHRL
                     RDLEAKALATALVEGIRSPAELSDAGRMQFFDLDPVPNYFFQRDPQVVLGNRVVISSM
                     ATAAREREPLLARTMFEHHSELAGLASCYEIDVPPSGAPQHNPRFQYPNLEGGDVLVA
                     SPEIILVGLSERTNRRGIEVLAEYLRLEETSFRHLVMVELPRRRSYMHLDTVFTFIDH
                     GTCLAYMPVIEPGGPESAHVYSVDLRAKDLTFTVRTSLLRALSSLGLELDVVPCGGPT
                     EAIDQQREQWTDGANAFAIAPGLILLYLRNRKTLEELGRRGWRVLSEDEALSGKHDLV
                     GGGRAVVSLQSNELSRARGGPRCMTMPLERDPL"
     gene            4732..6180
                     /gene="purB"
                     /locus_tag="VEL74_04310"
     CDS             4732..6180
                     /gene="purB"
                     /locus_tag="VEL74_04310"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006598613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HYG61780.1"
                     /translation="MPETPQNPLYERYASPEMARIFSASHRFHHWRNLWIVLAECQRE
                     LGLPVSGEQIAHLRAVAGDTDLARVAELERRTRHDVVAHLRHFAEQADRLGAGAGGVL
                     HLGATSAFLTDNTDVLLMREGLELLLARLAAAVTALAGFARRTRDIPALAYTHFQPAQ
                     LTTVGKRACLWLQDLVMDMAEVRHRLATLRCRGVKGTTGTQASFLTLFHGDHGKVREL
                     DRMVARRLGFAGSFPVTGQTYSRKQDSQVLAVLSGLAESCHKLGTDLRLLQGVGELSE
                     PFDAEQVGSSAMAYKRNPVRAERMCGLARRLMTDALNGPLNTSVQWLERSLDDSANRR
                     LVIPDSFLCADAIAGLAAHIAGGLVVREATVAARVARELPFMATETLLMESALRGGDR
                     QHLHERIRQYSLESQGRLERGGDNPLIGMIVDDPDFRLTRSEVEPWLDPVAFTGRSAR
                     QVDELLEEVVEPALAELKAAGLEAAEVAAPRV"
     gene            6379..7068
                     /locus_tag="VEL74_04315"
     CDS             6379..7068
                     /locus_tag="VEL74_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HYG61781.1"
                     /translation="MSTMRQDNSRCSALFNRGLLRLALAALVLVQIAAAGCSANKRPT
                     AAKSLDRPIERGVASWYGPRFHGRLTANGERYDMHEMTAAHPTLPFGTRVQVRNVRNG
                     QSALVRINDRGPFKKNRVIDVSFAAAKAIGLVSSGTALVDLYLAATGGGAAPSLSHYT
                     VQVGAFSDVDRAASLKANLSRIYPETRVHGDGTWSRVQVGSFDDRQQAETLRRELAAL
                     GMPSVVVSAAQ"
     gene            complement(7097..8743)
                     /locus_tag="VEL74_04320"
     CDS             complement(7097..8743)
                     /locus_tag="VEL74_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG61782.1"
                     /translation="MRVLLIDDNPDDRTLARRVLAKAFPGPPALEIQEIKNLEELGRA
                     LEAGGFGAVITDYQLHWSDGLTVLRRCKELYPGIPVLMFTNSGTEELAVEAMKAGLDD
                     YVLKQPQRFVRLPVALRAALDRAAERRAIEEERERFLAREREARAAAEAASAMKDEFL
                     ATLSHELRTPLNAIVGWAALLRSGTLEGEKLGRALEGIERNARSQARLIDDLLDVSAI
                     ISGRMSLEARPLDLVPVIEVALDSVRPAAEAKSVQLQIALDPYAGTVTGDPGRLQQVV
                     WNLLSNAVKFTPPGGSVRVMLARRDGQVRLTVSDTGQGIDPTFLPHAFEPFRQADGSI
                     TRAQGGLGLGLAIVRRLVELHGGTVHGESGGPGRGAAFHVDLPLRATAAERRAPEPLP
                     PAHGVGFECPPSLLDLDVLVVDDEPDARDLIAVMLEKCGARVRVAASVAEAMEAFTQS
                     PPDVLLSDIAMPGQDGYELIRRVRALPAHAGGRVPAAALTAHARAEDRRRTLLAGFNI
                     HVAKPFDPDELLAVVASLGGRVAAGEDGGLLETVPEEEGR"
BASE COUNT         1282 a         3222 c         3070 g         1222 t
ORIGIN      
        1 caggctgtga tcgtgggccg cagctcgatc gtcggcaagc cgatggccgg gctgctgcta
       61 cgcgagcact gcaccgtcac catctgccac tcgcgcaccc gcgacctcgc cgccatcacc
      121 cgcgaggcgg acatcctggt agccgccatc ggccgcgccg cgatgctcgg ccccgagcac
      181 gtgaaggagg gggcggtggt gatcgacgtc gggatcaccc gtgtccacga ccgggacgag
      241 gtggagcgcc tctacccggg cgacccggag cgccggcgcc atctcgagaa gcgcggctca
      301 acgctcatcg gcgacgtgga cttcacccgc gtcgcgccca tcgcttcggc catcaccccg
      361 gtgccgggcg gcgtcggccc gctcaccgtg gcgatgctga tcgccaacac cctgaaagcg
      421 gcgaagcgcc ggcaaggcct ctcgtgaccc gcgacggccg ccccctcctc gtcggactga
      481 ccggtggcct ggccagcggc aagtcgacgg tggcgggctg gctggcggac gccggcttcc
      541 gggtggtcga cgccgaccgc ctggtggccg acctctaccg ccccgggggc gccggggccg
      601 aggcggtgcg cgggctgttc ggtgacggca tgctcgacga gcagggggcc gtggtccccg
      661 cccgggtcgc cgaccggatc ttctcggacc cggaggcgcg gcgggcgctg gagggggcgg
      721 tccacccgct ggtgcgcgag cgctttcacg agatcgcccg cgagtcctcg caaggggtcg
      781 tggtgctcga agccaccctg ctggtggagg ccggctacag cccctcgttc gacctcatcg
      841 tcacggtgga agcgccggcg gtgacgcgcc tgcggcgggc gatggagcgg ggcatgagcg
      901 aggacgccgc ccgcgcccgc ctccttgccc agggagacgg cgaggtccgc cgcgaggcct
      961 cccaccggat catcgacaac tgcagcgacc tcccccacct gcgccaccag gtcgacgacc
     1021 tggtcatcga gctgcggcgg ctggcggacc agcaggccga gcagcgctcc gagccccggt
     1081 gagcctcccc cccttcgttc tggtcaccgc caatcccggc aagctcgtcg aggcccggcg
     1141 cctggcgggc cacgacgtcg agtctgccgc cgtcgacctc cccgagatcc agtcgctcga
     1201 cctggaggag gtgctccgcg ccaaggccga agccgcctgg tcgcgcctcg gacgacctct
     1261 ggtggtggaa gactccgggc tcgacctcac ggcgctcaac ggcttccccg gccccctcat
     1321 caagtggatg ctgaccgccg tgggagccga gggaatcgcc cgcgccggcc tctccctggg
     1381 agacccgcgc gccaccgccc gttgcatgct tttctataag gacgcgtacc gcgaggtgat
     1441 cgtcgaaggg gtcaccgccg gcaccctcgt cctccccggc cgcggcacgc gcggcttcgg
     1501 ctgggacccc gtcttcctcc ccgaaggcgg cgagctgacc ttcggcgagc tcgacggcgc
     1561 ggagaaggac gccgtcagcc acagggggaa ggcgtggcgg gctatgatta ggcggctggc
     1621 gggaggacgc cgcgagctga agcaggactg acagccccat cccctttccg ggaggccccc
     1681 gtaccgaagc gggaggacac tctccctcaa aggggaggat gccccaccca aaagggacca
     1741 cctcctcccg caaagagtac agccccctcc ccgatccggg aggcccatgt acccgaaacg
     1801 gtacggcccc ctccccttcc aggtacaccc cctccccaaa tcgggtacca ccccctcccg
     1861 gaaagggtac gggggcttcc ctcctctcca tggtccacgg tagagtctcc cgcaaagccg
     1921 tctttcccga caggagcgtc cgttcatgcc tcgcgtctct tcagccgtca cgatctcggt
     1981 gtgggaagcc ctcatcgatg gagtgaagca gttcgccgac gagctgcctc acctccagga
     2041 gctccaggcc gagctggaat ccaccctcac cgaggcgcgg cgctgtcaga ccgcgtacct
     2101 gaggtatcac gcgcgcgcca tgcaggaggc gcggcggatg cgggagatct ccgagaaggg
     2161 ccagcgcatc gagagccgga tccgcgccgg cctcaagagc gcccacgggg cggacagccc
     2221 caagctcatc cgctacggct tgcggccaca caaggggatg aggaagtcgg ccccgcggga
     2281 ggtggcgccc gcggagccgg agatcgacga agaggttgga gagacgagag acggcgaggg
     2341 ctaggcgatc tcgacgccgg ccatcagcca gagcaggatc gccttctgga catggaggcg
     2401 gttctccgcc tcgtcgtaga tgcggctctg cggcccgtcg gcgacctcgg cggccacctc
     2461 ctcgccgcgg tgcgccggca ggcagtgcag gaagatggcg tcctgccgcg cgccggacat
     2521 cagcttctcg tcgacgatga agccctggaa gtccttgagc cgcttctgcg cctcctgctc
     2581 ctgccccatc gaggcccaga cgtcggtgta gaccgccgac gcgtccttga ccgcctgccg
     2641 cggatcgtcg gtcagctcca gccgggtgcc ggcctcctcg gccgcccgcc gcgcctggcc
     2701 gagatagggc tctcgcacct cgtagccctt gggcgaggcg accgcgaagt ccatccccac
     2761 ccgcgaggcg gtcagcatca gcgacatggc catgttgttg ccgtcgccca cgtaggctag
     2821 cttgcggccc ttgaggtcgc tgccgaagac ctcggtcagg gtgaagtagt cggtcagcgc
     2881 ctggcagggg tggaggtcgt cggagaggcc gttgatcacc ggcacggtgc cgaagcgagc
     2941 caggtccgcc acggtctgat gggcgaagac acgggccatg atgccgtcga cgtagcgcga
     3001 cagcacctgc gccgtgtccg agatcggctc gccgcggccg agctggatgt cgcggctgga
     3061 caggaaaagg gcgtggccgc ccatctgcac catgcccacc tcgaagctca cccgcgtacg
     3121 ggtggaggac ttctcgaaga tcatgcccag gctcttgcgg gtcagggcgc cggaatattt
     3181 ctcggggttc gccttgacgt cggcggccag cttgaacagc cctgccacct ccgccgccga
     3241 gaggtcgtgg tccgagatca ggtgacgctg ggtcatcggg tctcctttat ggatagtgag
     3301 aaggaaagaa agaggaaggg ttcgggacgg ggcgccggcg tggaacgccg gcggatcaca
     3361 gcgggtcccg ctccaggggc atggtcatgc aacgcggccc cccgcgggcc cgcgacagct
     3421 cgttggactg cagcgacacc acggcgcgcc cgccgcccac caggtcgtgc ttccccgaca
     3481 gcgcctcgtc ctccgacagc acccgccagc cgcgccgccc cagctcctcg agcgtcttgc
     3541 ggttgcgcag gtagaggagg atgaggcccg gggcgatggc gaaggcgttg gcgccgtcgg
     3601 tccactgctc ccgctgctgg tcgatcgcct cggtcgggcc gccgcagggc acgacgtcca
     3661 gctcgaggcc gaggctggag agcgcccgca gcagcgacgt gcggaccgtg aaggtcaggt
     3721 ccttcgcccg caggtccacc gagtagacgt gcgccgactc cggccccccc ggctcgatga
     3781 ccggcatgta ggccaggcag gtgccgtggt cgatgaaggt gaagacggtg tccaggtgca
     3841 tgtacgagcg ccgccggggc agctccacca tgaccaggtg ccggaacgag gtctcttcga
     3901 gccgcaggta ctcggccagc acctcgatcc cccgccggtt ggtgcgctcg gacagcccca
     3961 ccaggatgat ctccgggctc gccaccagga cgtcgccccc ctccaggttc gggtactgga
     4021 agcgggggtt gtgctgcggc gcccccgacg gcgggacgtc gatctcgtaa cagctcgcca
     4081 agccggccag ctcggagtgg tgctcgaaca tggtgcgggc gaggagcggc tcgcgctcgc
     4141 gcgccgccgt cgccatcgac gagatcacca cccggttgcc cagcaccacc tgcgggtcgc
     4201 gctggaagaa gtagttgggc accgggtcga ggtcgaagaa ctgcatccgc ccggcgtcgg
     4261 acagctcggc gggcgagcgg atcccctcga ccagggcggt ggcgagggcc ttcgcctcca
     4321 ggtcgcggag ccggtgggcg acgctgaacg gcacgccgta ctcggcctcc agctcgtcga
     4381 ggagctttct ccgcaccgtc tcctcgcgca gcacgtcggc gagcaggtcg tgggcgtagt
     4441 gcacctggac accggcctgg cgcagcacgc cggtgaaggc gtcgtgctcc tcccgcgcct
     4501 cctcgccata gaggatgtcg tcgaagagca ggtgctccat gagggccggg accatccagt
     4561 cgatctcgcg gccgggacgg tggacgagga cgcgccgcag gcgtccgatc tcggtttcga
     4621 cgtgaatagg catggcgtgg atcgcgaaga ggcacccgga tgggcgcggc ggcggattgt
     4681 accagaccct cttgttatga tccccccatg cccgagacgc cgcccgagac cctgcccgag
     4741 acgccccaga accccctgta cgagaggtac gcctcgccgg agatggcgcg catcttctcc
     4801 gcctcccacc gcttccacca ctggcggaac ctgtggatcg tgctcgccga gtgccagcgg
     4861 gagctggggc ttcccgtctc cggggagcag atcgcccacc tgcgggccgt ggccggcgac
     4921 accgacctgg cgcgggtggc cgagctcgag cggcggacgc gccacgacgt ggtcgcccac
     4981 ctgcgccact tcgccgagca ggcggaccgc ctcggcgccg gcgccggcgg cgtcctccac
     5041 ctgggagcga ccagcgcctt cctcaccgac aacaccgacg tcctcctgat gcgcgagggc
     5101 ctcgagctgc tgctggcgcg cctcgccgcg gcggtgacgg cgctcgccgg cttcgcccgc
     5161 cgcacgcgcg acatcccggc cctcgcctac acccacttcc agccggcgca gctcaccacc
     5221 gtcggcaagc gggcctgcct ctggctgcag gacctggtga tggacatggc cgaggtccgc
     5281 caccgcctcg ccaccctgcg ctgccgcggc gtcaagggga cgacgggcac gcaggcgagc
     5341 ttcctcaccc tgttccacgg cgaccacggc aaggtgcggg agctcgaccg catggtggcg
     5401 cggcggctgg gcttcgccgg gagcttcccc gtcaccggcc agacctactc gcgcaagcag
     5461 gacagccagg tgctcgccgt gctctccggc ctcgccgaga gctgccacaa gctcggcacc
     5521 gacctgcgcc tgctccaggg ggtaggcgag ctctccgagc cgttcgacgc cgagcaggtg
     5581 ggctcgtccg cgatggccta caagcgcaac ccggtgcgcg ccgagcggat gtgcggcctg
     5641 gcgcggcggc tgatgaccga cgccctgaac gggccgctca acacctccgt gcagtggctg
     5701 gagcgcagcc tcgacgactc ggccaaccgc cggctggtga tccccgactc gttcctctgc
     5761 gccgatgcca tcgccgggct cgccgcccac atcgccggcg gcctggtggt gcgcgaggcg
     5821 acggtggcgg cgcgcgtcgc ccgcgagctg ccgttcatgg ccaccgagac cctgctcatg
     5881 gagtcggcgc tgcgcggcgg cgaccgccag cacctgcacg agaggatccg ccagtacagc
     5941 ctcgaatccc aggggcggct ggagcgcggc ggcgacaacc cgctgatcgg catgatcgtg
     6001 gacgacccgg atttccggct cacccgctcc gaggtggagc cctggctcga ccccgtcgcc
     6061 ttcaccggcc gctccgcccg gcaggtggac gagctgctgg aggaggtcgt cgagcccgcg
     6121 ctggccgagc tgaaagccgc cggtctcgag gccgccgagg tggcggcgcc ccgcgtctga
     6181 cctccccaga tatacgtcca catgaatagg cgcgccggcc ggcgcgcctt tccgcgcgca
     6241 gaccgtcggg acacgtcgcg tagcggggaa ctggatgaaa attccaggtg tgctactatc
     6301 ccggcagcgc aagctgtttc agcgctgggc ccagaggctc tcacgaagat ggccaaccac
     6361 caccttacgg agaacaccat gtcgaccatg cgccaagaca actctcgttg tagcgctctg
     6421 ttcaaccggg ggctgcttcg actcgccctc gccgctctcg tcctcgtgca gatcgcggcg
     6481 gccgggtgct cggccaacaa gcggcctacc gccgccaagt cgctggaccg ccccatcgag
     6541 cgcggcgtcg cctcctggta cggccccaga ttccatgggc gcctgaccgc caacggcgag
     6601 cgctatgaca tgcacgagat gacggccgcc cacccgacgc tgcccttcgg cacgcgcgtc
     6661 caggtccgca acgtccgcaa cgggcagtcc gccctggtcc gcatcaacga ccgcgggccg
     6721 ttcaagaaga accgggtgat cgacgtctcc ttcgccgcgg cgaaggcgat cgggctggtc
     6781 tcctcgggca cggccctggt ggacctctac ctggccgcca ccggcggcgg cgccgctccc
     6841 agcctctccc actacacggt ccaggtgggg gccttcagcg acgtcgaccg ggccgcctcc
     6901 ctcaaggcga acctgtcccg gatctatccc gagacccggg tccacggtga cggcacctgg
     6961 agccgcgtcc aggtcggctc cttcgacgac cgccagcagg ccgagaccct gcgccgcgag
     7021 ctggccgccc tgggcatgcc ctcggtggtc gtctccgccg ctcagtaggt gaaacgaagg
     7081 ggagcgcccg ctcccctcac ctcccctcct cttccggcac cgtctccagc aagccgccgt
     7141 cctcgccggc cgccacccgg ccgcccaggc tcgccaccac cgccagcagc tcgtcgggat
     7201 cgaacggctt ggcgacgtgg atgttgaagc cggcgaggag ggtgcgccgg cggtcctcgg
     7261 cccgggcgtg ggcggtcagg gcggcggcgg ggacgcgccc gccggcgtgg gccggcaggg
     7321 cccgcacccg gcggatcagc tcgtagccgt cctgcccggg catggcgatg tcggagagca
     7381 gcacgtccgg gggggactgg gtgaaggcct ccatcgcctc ggccacggag gcggccaccc
     7441 gcacccgggc gccgcacttc tcgagcatca ccgcgatgag gtcgcgggcg tccggctcgt
     7501 cgtccaccac caggacgtcg aggtcgagga ggctcggcgg gcactcgaac ccgaccccat
     7561 gagccggcgg gaggggctcg ggcgcccggc gctccgcggc ggtggcccgc aacggcaggt
     7621 ccacatggaa ggccgccccg cgtcccggcc cgccgctctc gccgtgcacg gtgccgccgt
     7681 gcagctccac cagccgccgc acgatggcca ggcccagccc cagccccccc tgggcgcggg
     7741 tgatcgagcc gtccgcctgg cgaaagggct cgaaggcgtg gggcaggaag gtggggtcga
     7801 tcccctggcc cgtgtcgctc acggtgagcc ggacctggcc gtcccggcgc gccagcatca
     7861 cgcggacgga gccgccgggc ggcgtgaact tgacggcgtt cgagagcagg ttccagacca
     7921 cctgctgcag acgcccggga tcgccggtga cggtgccggc gtagggatcg agggcgatct
     7981 ggagctggac cgacttcgcc tccgccgccg ggcggaccga atcgagggcc acctcgatca
     8041 ccggcaccag gtccagcggc cgggcctcga ggctcatccg gcccgagatg attgccgaga
     8101 cgtccagcag gtcgtcgatc agccgcgcct gcgagcgggc gttccgctcg atgccctcga
     8161 gggcgcgccc gagcttctcc ccctcgagag tgccggagcg cagcagcgcc gcccagccga
     8221 cgatggcgtt gagcggcgtg cgcagctcgt gggagagggt ggccaggaac tcgtccttca
     8281 tggcgctcgc cgcctcggcc gcggcgcggg cctcgcgctc gcgggcgagg aaccgctccc
     8341 gctcctcctc gatggcgcgc cgttcggcgg cccggtcgag cgcggcgcgc agggcgaccg
     8401 gcaggcggac gaagcgctgc ggctgcttga gcacgtagtc gtcgaggccg gccttcatcg
     8461 cctcgaccgc cagctcttcg gtgccgctat tggtgaacat caggaccggg atgccggggt
     8521 agagctcctt gcaccggcgg aggacggtga ggccgtcgct ccagtggagc tggtagtcgg
     8581 tgatgacggc gccgaagccg ccggcctcca gggcgcggcc gagctcctcc aggttcttga
     8641 tctcctggat ctcgagagcg ggcgggccgg ggaaggcctt cgcgaggacg cgccgcgcca
     8701 gggtccggtc gtcggggttg tcgtcgatga ggaggacgcg catgacctct ccgctactcc
     8761 ggcgccctct gcggcagctc gatccagaac cgtgtg
//
LOCUS       DASPJI010000164         5048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_638047, whole genome shotgun sequence.
ACCESSION   DASPJI010000164 DASPJI010000000
VERSION     DASPJI010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638047"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VEL74_04325"
     CDS             complement(<1..452)
                     /locus_tag="VEL74_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HYG61783.1"
                     /translation="MSTSTQAHDRQFDIASIMGGLYGDGIIGFKGAFDRSWVRELRED
                     IEVLFQEALRRPGGAVGRGPKRHYVEIHPERLRGFVDLATHPWVAAVCEAVLGPDYKI
                     VEIGFDIPGPGAAYQPWHRDFPSPPETLIGRRLNSLAFNVTTVDVVEE"
     gene            complement(554..1153)
                     /gene="msrA"
                     /locus_tag="VEL74_04330"
     CDS             complement(554..1153)
                     /gene="msrA"
                     /locus_tag="VEL74_04330"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HYG61784.1"
                     /translation="MKTRMMRISLLLGLLAVAALPASGAKLAKATFAGGCFWCMEPPF
                     DKLPGVVSTTSGYIGGQKKNPTYEQVSAGGTGHAEAVEIVYDPAKVSYEKLLEVFWHN
                     VDPTTPDRQFCDVGDQYRTGIFVHDEAQKRLAETTKARVARQLGKPVVTEIVSAGPFY
                     PAEEYHQDYYEKNPLRYKLYRFNCGRDQRLEQLWGKKGE"
     gene            complement(1176..1979)
                     /locus_tag="VEL74_04335"
     CDS             complement(1176..1979)
                     /locus_tag="VEL74_04335"
                     /inference="COORDINATES: protein motif:HMM:NF024107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYG61785.1"
                     /translation="MKIRWQTIRKVWATTGAILFVVFTLWNLISYQSWGVDEAVLRSD
                     GRVSVEPRPGYTAFLPRAGARPVGLVFYPGALVDPEAYAPLARTVAESGYPVFLLELP
                     LRLAPSLSSEVEVYSRTLSIMKRETAVRRWIVGGHSKGGAIACRFAVRNTRSPYGLLL
                     VGSSHPREIDMSDLPFEVTKIYATRDGLASEEEVRHYARNLPEDTHWVRIEGGNHCQF
                     GWYGFQFGDRPAAISRARQQDLLAAAVIDALRRAEVEPEGEPMSTGGAY"
     gene            complement(1981..2751)
                     /locus_tag="VEL74_04340"
     CDS             complement(1981..2751)
                     /locus_tag="VEL74_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HYG61786.1"
                     /translation="MLFDEAHHNLHTSGGRYKPFAELITEDGYTVTPNTAPFSADTLQ
                     GFDVLVISNARGAGQEVPMEERSRPAFTTAEVEAVGDWVEAGGALLLIADHYPIGSAN
                     RPLGDRLGVSMSNGWTEDPANRDPVLQDLLFSRDNGLLGDHPITRGRSPQERIGRVVS
                     FLGQSLAGPPGSVPLLRLAPSAVDELPPDRKQSVSAAGRSQGLAMTLGKGRVVVMGEA
                     AMLTSQTFDGMVLGFDSPGIDNRQLVLNILHWLSGELD"
     gene            2999..3181
                     /locus_tag="VEL74_04345"
     CDS             2999..3181
                     /locus_tag="VEL74_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61787.1"
                     /translation="MRLGKVLGLLLLLALTATAGVSAGPGGCLHGDCNTDCGECGGRL
                     YFCDSEGYPVCACYNC"
     gene            3292..3639
                     /locus_tag="VEL74_04350"
     CDS             3292..3639
                     /locus_tag="VEL74_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014993503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HYG61788.1"
                     /translation="MDRDRIYKVVFYHQGNVVELYARRVGQGELFGFIEVEELIFGAR
                     SEVVVDPTEEGLRAEFGGARRLFLPMHAILRIEEVEREGTARMRSVPAPRNEGNIVRP
                     FPLPFFPPGDPSK"
     gene            3949..4884
                     /locus_tag="VEL74_04355"
     CDS             3949..4884
                     /locus_tag="VEL74_04355"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61789.1"
                     /translation="MAEKLRIGILTTPEASPDKPWVHQDEIAGDEVVSTLLVAGHDVV
                     RLGSVSDLAWRLDELRERIDLVLNLDPGLEAAVFLDWAGFPATGSPPSVIGLAAHRLA
                     AKLEVERAGVVTPAAVVIPGPHEPGDLGLESLHYPALVKPLMRRQPGEVSAACRVEDA
                     GAARQQARRMVDEGRGPALVETLVHGVEVQVPILATPEPKALGVLAVAIEGQPLDGDA
                     ILGPDVPEYRLVEPPSRVDESRVRKAAVQAFTALGLRDYGRIDLRIDADGTPWFLAAH
                     PVPGLERDGAFGCAAPLGRIAGAIVRAAASRAQRN"
BASE COUNT          778 a         1728 c         1753 g          789 t
ORIGIN      
        1 atctcctcga ccacgtcgac agtcgtgacg ttgaacgcca gcgagttgag gcgccggccg
       61 atcagcgtct ccggcggcga agggaagtca cgatgccacg gctggtaggc ggcgcccggg
      121 cccgggatgt cgaaaccgat ctcgacgatc ttgtagtccg ggccgagcac cgcctcgcag
      181 accgcggcga cccacgggtg ggtggcgagg tcgacgaagc cgcgcagccg ctccgggtgg
      241 atctcgacgt agtggcgctt gggcccgcgc cccaccgcgc cgccggggcg ccggagcgcc
      301 tcctggaaca ggacctcgat gtcctctcgc agctcccgca cccagctccg gtcgaaggcc
      361 cccttgaatc cgatgatgcc gtctccgtag agaccgccca tgatgctcgc gatgtcgaac
      421 tgccgatcgt gcgcctgcgt gctggtgctc atacatcctc gtcgggagtg atctccggtt
      481 acagccgacg agcgtatgac gctgcgagac ggcctccgaa ctaccttttg agtctaacct
      541 gatctgaacc tacctactcc cccttcttcc cccagagctg ctcgaggcgc tggtcgcggc
      601 cgcagttgaa gcggtagagc ttgtagcgga gggggttctt ctcgtaatag tcctggtggt
      661 actcctcggc ggggtagaac ggaccggcgg acacgatctc ggtcaccacc ggcttgccga
      721 gctgccgcgc cacgcgggcc ttggtcgtct cggcgagccg cttctgggcc tcgtcgtgga
      781 cgaagatgcc ggtccggtac tgatcgccca cgtcacagaa ctggcggtcc ggagtggtgg
      841 ggtccacgtt gtgccagaag acctccagca gcttttcgta gctgaccttc gccgggtcat
      901 agacgatctc cacggcctcg gcgtggccgg tgccgccggc ggagacctgc tcataggtgg
      961 ggttcttctt ctggccgccg atgtagcccg aggtggtcga gaccaccccc ggcagcttgt
     1021 cgaagggggg ctccatgcac cagaagcagc cgccggcgaa ggtcgccttg gcgagcttgg
     1081 cgccggaggc cggaagggcg gccaccgcga gcaggcccag aagcagcgat attctcatca
     1141 tcctggtctt catcgtcgtc atctcctgtc cgctcctagt acgcgccgcc ggtggacatc
     1201 ggttcaccct cgggttcaac ctcagctcgg cgcagggcgt cgatcacggc ggcggcgagg
     1261 agatcctgct ggcgggcccg gctgatggcg gcggggcgat ccccgaactg gaagccgtac
     1321 cacccgaact ggcagtggtt gcccccctcg atccgcaccc agtgggtgtc ctccggcagg
     1381 ttcctggcgt agtgtcggac ctcctcctcg ctcgccaggc cgtctcgggt cgcgtagatc
     1441 ttggtgacct cgaagggcag gtccgacatg tcgatctccc gcggatggga gctcccgacc
     1501 aggagaagcc cgtaggggct ccgggtattc cgtaccgcga aacggcacgc gatcgccccg
     1561 cccttggaat ggccgccgac gatccagcgc cggaccgcgg tctcccgctt catgatggag
     1621 agggtacggc tgtagacctc gacctcggag gacagggagg gtgcgaggcg gaggggcagc
     1681 tcgagcagga agaccgggta cccgctctcg gcgacggtcc tggcaagagg cgcgtacgcc
     1741 tccgggtcca ccagggcgcc ggggtagaag accagtccca ccggccgggc gccggcccgg
     1801 ggaaggaagg ccgtataccc ggggcggggc tcgaccgaca cccggccgtc gctgcgcagc
     1861 acggcctcgt ccacccccca ggactggtag gagatcaggt tccagagcgt gaagacgacg
     1921 aagaggatgg cgccggtggt ggcccagacc ttccggatcg tctgccaccg gatcttcatc
     1981 tcagtcgagc tcgccggaca gccagtggag gatgttgagg acgagctgcc ggttgtcgat
     2041 gcccgggctg tcgaagccca ggaccatgcc gtcgaacgtc tggctggtga gcatggcggc
     2101 ctcgcccatc accaccaccc gccccttgcc cagcgtcatc gcgagcccct ggctgcggcc
     2161 tgccgcggag accgactgct tccggtcggg cggcagctcg tccaccgccg agggcgcgag
     2221 ccgcagcagg gggacactgc ccggcggccc ggccagggac tgtcccagga agctcaccac
     2281 gcgcccgatc cgctcctgcg gcgaccggcc gcgggtgatc gggtggtctc ccaggaggcc
     2341 gttgtcgcgc gagaagagga gatcctggag gaccgggtcg cggttggcgg ggtcctcggt
     2401 ccagccgttg ctcatcgaca cgccgaggcg gtcgccgaga ggccggttgg cggagccgat
     2461 gggatagtga tcggcgatca ggaggagcgc gccgcccgcc tccacccagt cccccaccgc
     2521 ctccacctcg gccgtggtga acgccggccg gctccgctcc tccatcggca cctcctggcc
     2581 cgctccgcgg gcgttggaga tcaccagcac gtcgaaccct tgcaaggtgt cggcgctgaa
     2641 cggcgcggtg ttcggcgtga ccgtgtagcc gtcctccgtg atcagctcgg cgaagggctt
     2701 gtagcgcccc ccgctggtat ggaggttgtg gtgggcctcg tcgaagagca cccggcggcc
     2761 tgatacctgc ggggacggac cggcaggacc gtcagatggg gtcgaggccg atgagcaggc
     2821 gaggaggaga acgagaggaa cgaaggcagc gaggcggtgt cggaacgggc gacggctcga
     2881 tcgcatggtc agatctccag agacagggct ccagaatagg acatacgtga ggctcccgtc
     2941 agggttgccc gggcaccttc ttaagagggc aatgcccatt ctgcgaaagg agaagaccat
     3001 gagactcgga aaggtcctcg gactgctgct cctgctggcc ctgacggcca ccgccggggt
     3061 gagcgccggg cccggcggct gcctccacgg cgactgcaac accgactgcg gcgagtgtgg
     3121 agggcggctc tacttctgtg actccgaagg ctatcccgtc tgcgcctgct acaactgcta
     3181 gaccgaaccc ggggaggagc gctcctcccc accctcctct ccctcccacc cgccttccat
     3241 ggtctctgat acgatggcgc ccgaaactat tgcgcagagc gtcggacgtc aatggataga
     3301 gaccgcatct ataaggtcgt cttttaccac caggggaacg tggtggagct gtatgcccgc
     3361 cgcgtaggcc agggggagct gttcggcttc atagaggtcg aggagctgat cttcggcgcc
     3421 cgctcggagg tggtggtcga tcccacggag gaggggctgc gcgccgagtt cggcggcgcc
     3481 aggcggctgt tccttcccat gcacgccatc ctccgcatcg aggaggtgga gcgcgagggc
     3541 accgcccgca tgcgctcggt gccggcccca cggaacgagg gaaacatcgt gcgccccttc
     3601 cctctcccct tcttcccgcc gggcgatccc tccaagtagc agagcgggcc gtctcctcga
     3661 aaaaagagtg ggggctcgcg gctccgatcc atctcgtccg cgagcccacg gagggaagag
     3721 tgatgtacag agtctgagcc cctcttcccg gcgatacaac cgcatggatc ggcagttctt
     3781 cgcggcccgg agcgccaggg gggccggcga cccgcacatt cgtgcaggga ggcgcccgtc
     3841 cgcggcctcg gcagcgtccc tgagccgggg atcggcagaa tcccgtgaag ctgtctcttg
     3901 gattcaccga aaaatgacct cgtcacgcgc ctgcactaca atcaccgcgt ggccgaaaag
     3961 ttgcgcatag ggattctaac gacgcctgag gcatccccgg acaagccctg ggtccatcag
     4021 gacgagatcg ccggtgacga ggtggtctct accctgctgg tcgccggaca cgacgtcgta
     4081 cgcctcggca gcgtctccga cctggcgtgg aggctcgacg agctccggga gcggatcgat
     4141 ctggtgctca acctcgatcc cggcctcgag gcggccgtct tcctggactg ggcagggttc
     4201 ccggcgaccg gctcgccccc ctcggtgatc ggcctcgccg cccaccggct cgccgccaag
     4261 ctcgaggtgg agcgggccgg cgtcgtcacg ccggcagccg tggtgatccc cggtccccac
     4321 gaacccggcg acctcggcct ggagtcgctc cactaccccg ccctggtcaa gccgctgatg
     4381 cggcggcaac ccggcgaggt ctccgccgcc tgccgggtgg aggacgccgg ggccgcccgg
     4441 cagcaggcgc ggaggatggt ggacgagggg cgcgggccgg ctctcgtcga gaccctggtg
     4501 cacggcgtcg aggttcaggt ccccatcctc gccacgccgg agccgaaggc gctgggagtc
     4561 ctggccgtcg ccatcgaggg gcagccgctc gacggcgacg cgatcctcgg tcccgacgtt
     4621 cccgagtatc ggttggtgga gcccccctcc cgggtcgacg agagccgggt acggaaggcg
     4681 gcggtccagg ccttcacggc cctgggcctg cgcgactatg gccggatcga cctgcggatc
     4741 gatgcggacg gcaccccctg gttcctcgcc gcccatccgg tgcccgggct ggagagggac
     4801 ggcgccttcg gctgcgccgc gcctctcggg cgcatcgccg gcgcgatcgt gcgggccgcc
     4861 gcgagccggg ctcagcggaa ctgaacgtcg ccccgcgccc agagcgaccg gtagatccag
     4921 acctggacct tggtggtccg ttccaggacc ctggcctggc gcaggagacc cggctcgggc
     4981 gtcccgcgcg ccaccggccc gatcttctga taggcctgca ggcgcaggcg cgccaggcag
     5041 gcgagggc
//
LOCUS       DASPJI010000165        10987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_241323, whole genome shotgun sequence.
ACCESSION   DASPJI010000165 DASPJI010000000
VERSION     DASPJI010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10987)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10987
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241323"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VEL74_04360"
     CDS             <1..634
                     /locus_tag="VEL74_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HYG61790.1"
                     /translation="VGGDGTLLAASHWVTGAALLGVNSAPQSSVGYLTRTRRASLAGD
                     LAKIAKGTLVPQEVARIEVELEGKRLPPALNDVLLAHESPAATSRYRVHLGRRAEDHR
                     SSGLWVASPAGSTAGIRSAGGTPMPLDVRRLQFRARELYRVRDRGVVLEAGFVEDGHE
                     LAVESAMEAGWLFVDGSRMPVRFPFGARATFRIAEHPLRLFANPDRWAPE"
     gene            complement(674..1405)
                     /locus_tag="VEL74_04365"
     CDS             complement(674..1405)
                     /locus_tag="VEL74_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYG61791.1"
                     /translation="MSDDLDSRLHFRQLLAGQQVAVHNPYAGRMANFMYLLGDSQTRE
                     AFIVDPAWDVRSLLDVAEADGYTVRGALVTHYHPDHVGGDLFGMSVEGLARLMEHAAM
                     PVYVNKHEADGLKKVTGLAESDLRRVDDGDTARVGEIDVRFLHTPGHTPGSQCFLIGD
                     RLVAGDTLFVQGCGRVDLPGGDPDAMYHTLTGKLAKLPPETVLYPGHHYGPTPTSTIG
                     NELEQNYYLRVRSLDDWHRLMNRGR"
     gene            1455..2690
                     /locus_tag="VEL74_04370"
     CDS             1455..2690
                     /locus_tag="VEL74_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61792.1"
                     /translation="MSRRTKPLLPLATLLVLSLTALRAQAPTEVQQIERALSVAPEHA
                     GMWIELAAAHARAGNKAEAAKWLEKAVASGLDFELAGNPAYSALREGPEYKALLTRAE
                     ANRRVVDRSRVAFRLVEKDLVPEGIAHDPKTGAFFVGSLYKRKIVRVGKDGKATDFTA
                     AGQGGLWDVLGMKVTPPPAPSGSAPAPAGGPASRTAGRVSSGSTSTRGSSSASTSCRA
                     SRSPICSTTWPSARGARCSSPTARPAWSTGCGPARPPSTLVAPGTFIYPNGIAISPDA
                     KRLFVADFTKGISMVDVATGQVRPLPHPEKIHIAGVDGLYFQEGSLIAVQNGAGAARI
                     VRFRLNAALDAVEAEEILESRNPLFVIPTTGTLAGGSFFYIANSQLRTLDDQGNLKPD
                     ARLVEPVVLEVPLARAAIP"
     gene            2736..4505
                     /gene="polX"
                     /locus_tag="VEL74_04375"
     CDS             2736..4505
                     /gene="polX"
                     /locus_tag="VEL74_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HYG61793.1"
                     /translation="MENVEIARTLQEIADILEIKNENPYRIRAYRNAARTVSVQMKPL
                     RRVVEEGGRLTDLPGIGKEMANHIREMVETGTLAYRDELTAEVPRSLVDLVRLPSVGP
                     KKAKKLWDELGICSLEELEAAAKTGKIAGVAGFGAKTQERILAGLAEHREHASRYLLA
                     DAEVFVEPLVDYLRAVPGVERLEVAGSYRRRRETVGDIDLLAVASDTAPVMRAFAGYP
                     QRDKVLMDGDTRSTITLGSGLQVDLRAVPPECYGAALVYFTGSKEHNVKLRRRGVERG
                     LRISEYGVFRVGGAEKGDQEGVFHGERIAGATEEEVYASVGLAWIPPELREDRGEIEA
                     AAAGRLPRLLEAGDLRGDLHMHSTWSDGRNSIEEMVEACAARGYEYMVISDHSKALSM
                     VNGLDAYRLKLQWKEIDEVQARHPEIRILKAMEIDILADGSLDLEDEMLAGLDLVLVS
                     LHSGLGMPGDEQTARVLKALEHPEVNVFCHPTARLINRRRPVEMDMDRVLSRAAELGV
                     AVEINCNPHRLDLKDTHARLAKELGCKLVVSTDSHRTRELDLVRYGVEQARRAGLEPG
                     DVLNTLPLKKLLKAVERKSAVRV"
     gene            4564..6147
                     /locus_tag="VEL74_04380"
     CDS             4564..6147
                     /locus_tag="VEL74_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYG61794.1"
                     /translation="MKQPQLSGVSRALALAGLAMALLAGPATSQDLLKSFEERTTVHT
                     LKNGWTFLIVERPVVPVFSFCTLADVGSAQEVPGITGLAHMFEHMAFKGSPNLGTRDA
                     AGEKKALEELEAAYQAYNRERLAARPDSAKVERLLAAFRDKQKAAAAFVVQNEFDDVI
                     TREGGAELNATTDTDKTRYFFSLPSNKTELFAFLESERFLHPVFREFYEERDVVQEER
                     RASVESQAVGRMMEQFTAAAFSAHPYGNDIGGHMSDLQSFTITDAEAFFRTYYAPENL
                     LTVVVGDVRAQELIPLVERYFGRIPARPGPAPLRTVEPPQIVEKTVVLEDPQAQPFYM
                     EGYKKPANTHPDQPVYDAIDDILTGGRTSRLYRALVRDKKLAANVSGYTGFPGTKYPN
                     LITFFVTPAFGVQNEQVQAALRDELERLKREDVSDAELARYKTRAKAQLLRQLRTNVG
                     IAIEFAEYQNEYGDWRELFRSLDRLEKVTKEDIRRVANQVFQKSSRTVGMVVHKPQET
                     PGSPQSSPAGAARTGGGAR"
     gene            6158..7642
                     /locus_tag="VEL74_04385"
     CDS             6158..7642
                     /locus_tag="VEL74_04385"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYG61795.1"
                     /translation="MKLIRNHALAALCVLALLLVLPAGAGADAQVATRVDQLKYPPLR
                     PFEIPQPQRVVLDNGMVVMLLEDRELPLVEVTALVRSGARLDPADKVGLSSLASNVLR
                     IGGTTRMPSDQLDDWLEDRAATIEAGTADSLTRVFMTSLSQDFPEVLRVFGDVLRRPA
                     FAADRLEVARNQAITAVSRQNENPMGIAFREFNKVVYGADSPLAWTPTHATLRAVSRD
                     DIVAWHRRYFHADRVVLGLVGDFRSEEALTLVRQVFGDWPRGPAEAPTETPYRKQPSP
                     GVFFVEKSDVPQSQVVMGHLGVRRDDPDYYALEVANQVLSGSFASRLFSNVRTRKGLA
                     YTVFGGVFSAFDFPATTLLYMSTKKETTGAGIDSLLKEARDVVRDLPPTDEEVAKAKQ
                     GLLNSFVFRSDSPRRVLASLLNYEAYGYPLDWLTRYPKGIEAVTAEQVRQAAARHLRP
                     EEFAILVVGPAEGQDRPLTDFGKVTPVELTAPGAPPARPAPTRK"
     gene            8072..9199
                     /locus_tag="VEL74_04390"
     CDS             8072..9199
                     /locus_tag="VEL74_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018480102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYG61796.1"
                     /translation="MKGRVLITGGAGFIGSHLADELLEHGYRVRALDDLSEQVHGPAA
                     ERPGYLHPDVELVRGDVRDPRVVRAALEGVDAVYHFAAVVGVGQSMYELERYTSVNNV
                     GTAVLLEALIEKPVGRLVVASSMSIYGEGLYRNAQGGLVAGTERDREQLKAHDWELRD
                     ERGEALAPLPTPETKAPTLSSLYALSKYDQERMCLMIGRAYDIPAVALRFFNVYGTRQ
                     ALSNPYTGVLAIFASRLLNGNPPLIFEDGRQMRDFVHVSDVARACRLALEVPEAPSVD
                     GQVLNVGSGRQYTVLEIARSMAEVLGREDIEPEITGKYRVGDIRHCFADISLARRLLG
                     YEPQMPFEQGLIELASWLEGQVAYDRVAEARAELAARGLTV"
     gene            9268..10350
                     /locus_tag="VEL74_04395"
     CDS             9268..10350
                     /locus_tag="VEL74_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYG61797.1"
                     /translation="MAREKVVLVTGGSGFIGTNLADRLASSGRRVRLLDNLSRRGVEE
                     NLRWLKKTHGDRISIEVADVRDPFAVRRAMAGVETVFHFAAQVAVTTSLLDPVHDFEV
                     NARGTLNVLEAARAQEEPPRVLFTSTNKVYGGMEDVALRLEGRRYEPCSPAVRAQGFS
                     EARPLDFHSPYGASKGAADQYVADYARSYGLRTVVFRMSCIYGPHQFGNEDQGWVAHF
                     LIRAMEDQPITLYGDGMQVRDVLYVDDLVRAFLLAERNIEALSGQAFNMGGGPANTVS
                     LLELIDLIHELQGRRPEVFFEDWRTGDQRYYVSDIRKFEAATGWSPLVSARQGLGNLY
                     NWLMESRGLGEPRLVAASGSPESLAL"
     gene            10499..>10987
                     /locus_tag="VEL74_04400"
     CDS             10499..>10987
                     /locus_tag="VEL74_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYG61798.1"
                     /translation="MTESSNPLPRKVLMTADTVGGVWTYALELARGLGERGVEVALAA
                     MGGPLSDLHREKADRIPRLKVFESTFKLEWMEDCWRDVEKAGEWLLRLEDRICPDLVH
                     LNGYAHAALPWKAPKMVVGHSCVLSWWQAVKGEAAPESWDRYRAEVTAGLAAADLVAA
                     PTA"
BASE COUNT         1725 a         3930 c         3736 g         1596 t
ORIGIN      
        1 ggtgggcggc gacggcaccc tcctcgccgc ctcgcattgg gtgaccgggg ccgcgctgct
       61 cggggtcaac tccgcgccgc agtcgagcgt gggctatctg acgcggaccc ggcgggcgag
      121 cctggcggga gacctggcca agatcgccaa ggggacgctg gtgccgcagg aggtcgcccg
      181 catcgaggtg gagctcgagg ggaagcggct gccgccggcg ctgaacgacg ttctcctcgc
      241 gcacgagtcg ccggcggcga ccagccgcta ccgggtgcac ctcggccggc gggcggagga
      301 ccaccgcagc tccggcctgt gggtggcgtc gccagcgggc tcgacggccg gcatccgctc
      361 cgccggcggc actccgatgc ccctcgacgt gcgccggctc cagttccgcg cccgcgagct
      421 ctaccgtgtg cgggaccgcg gcgtggtcct ggaggccggt ttcgtggaag acgggcacga
      481 gctggcggtc gagtcggcca tggaggcggg ctggctcttc gtcgacggct cccggatgcc
      541 ggtccgcttc cccttcggcg cccgcgccac cttccgcatc gccgagcacc ccctccgcct
      601 cttcgcgaac ccggaccgct gggcgccgga gtaggggggg cgccccgccg ggagcgcgga
      661 cggtccgaac ggctcagcgg ccccggttca tcaggcggtg ccagtcgtcc agcgaacgga
      721 cgcggaggta gtagttctgc tccagctcgt tcccgatggt cgaggtgggc gtcgggccgt
      781 agtggtggcc gggatagagc acggtctcgg gaggcagctt ggcgagcttg ccggtcagcg
      841 tgtggtacat ggcgtcgggg tcccctcccg gcaggtcgac ccggccgcag ccctggacga
      901 acagggtgtc ccccgccacc aggcggtcgc cgatcaggaa gcactggctg cccggcgtgt
      961 ggcccggagt gtggaggaaa cgcacgtcga tctcccctac ccgggcagtg tcgccgtcgt
     1021 ctacccggcg caggtcgctc tccgcgagcc ccgtcacctt cttgaggccg tccgcctcat
     1081 gcttgttgac gtagaccggc atcgcggcgt gctccatcag ccgggcgagc ccctcgaccg
     1141 acatgccgaa caggtcgccg cccacgtggt ccggatggta gtgggtgacc agcgccccgc
     1201 gcaccgtgta gccgtcggcc tcggccacgt ccagcaggct gcggacgtcc cacgccgggt
     1261 cgacgatgaa tgcctcgcgg gtctgggagt cccccaggag gtacatgaag ttggccatcc
     1321 ggccggcata ggggttgtgg accgccacct gctggccggc caggagctgc cggaaatgga
     1381 gccgggaatc gaggtcgtcg ctcatgggcc gatcttctcc cagatggtgt agcgttgcca
     1441 gcctggaggt tccgatgagt cgacgcacga agccccttct cccccttgcc accctgctcg
     1501 tcctgtccct gaccgccctg cgggcgcagg cgcccaccga ggtccagcag atcgagcggg
     1561 cgctgtcggt tgccccggag cacgccggca tgtggatcga gctggccgcg gcccacgccc
     1621 gggcgggcaa caaggccgaa gccgcgaaat ggctggagaa ggcggtcgcc agcggcctcg
     1681 acttcgagct cgccggcaac ccggcctact cggcgctgcg cgagggtccg gagtacaagg
     1741 cgctcctcac tcgcgccgag gccaatcggc gggtcgtcga tcgcagccgg gtggcgttcc
     1801 ggctggtaga gaaggacctc gtccccgagg ggatcgccca cgacccgaaa accggcgctt
     1861 tcttcgtcgg cagcctctac aaacgcaaga tcgtccgcgt cggcaaggac ggcaaggcca
     1921 cggacttcac ggccgcaggc cagggcgggc tctgggacgt cctcggcatg aaggtgaccc
     1981 cgccacccgc tccctctggg tctgctccgg cgccggcggg cgggccggcg agcaggacgg
     2041 ctggtcgggt ctcttccggt tcgacgtcga ctcggggaag ctcctcggca agtacgagct
     2101 gccgggcaag ccgcagcccc atctgttcaa cgacctggcc ttcggcacgg ggggcgaggt
     2161 gttcctcacc gacagcaagg ccggcatggt ccaccggctg cggcccggcc aggccgccct
     2221 cgaccctggt ggcgccgggg accttcatct atcccaacgg catcgccatc tcgccggacg
     2281 cgaagcgcct gttcgtcgcc gacttcacca aggggatctc catggtggac gtagccaccg
     2341 gccaggtccg ccccctcccc cacccggaga agatccacat cgccggcgtc gacggcctct
     2401 atttccagga ggggagcctg atcgccgtcc agaacggcgc cggggccgcg cggatcgtcc
     2461 gcttccggct caacgccgcg ctggacgccg tcgaggccga ggagatcctg gagagccgca
     2521 acccgctgtt cgtgatcccg accaccggca ctctcgccgg cggctccttc ttctatatcg
     2581 ccaacagcca gctccgcacc ctggacgacc aggggaacct caagccggac gcccggctgg
     2641 tggagccggt ggtcctcgaa gtccccctgg cgcgcgccgc gataccatga gccctcaggg
     2701 cgtcgccccc cggaggtgac gcgggagagg ggcccatgga gaacgtcgag atcgcgcgca
     2761 cgctccagga gatcgcggac atcctcgaga tcaagaacga gaacccgtac cgcatccgcg
     2821 cctaccgcaa cgccgcccgc accgtctcgg tgcagatgaa gccgctgcgc cgggtggtcg
     2881 aggagggggg ccgcctcacc gacctccctg gcatcggcaa ggagatggcc aaccacatcc
     2941 gggagatggt cgagaccggc accctcgcct accgcgacga gctgaccgcc gaggtgcctc
     3001 gctcgctggt cgacctcgtg cgcctgccga gcgtcggccc gaagaaggcg aagaagctgt
     3061 gggacgagct cggcatctgc tcgctcgagg agctggaggc cgcggccaaa acggggaaga
     3121 tcgccggcgt cgccggcttc ggcgccaaga cccaggagag aatcctcgcc ggccttgccg
     3181 agcaccgcga gcacgccagc cgctacctgc tcgccgacgc cgaggtgttc gtcgagccgc
     3241 tcgtcgacta cctgcgggcg gtccccggcg tcgagcgcct ggaggtggcc ggcagctacc
     3301 ggcggcggcg cgagaccgtg ggggacatcg acctcctagc cgtcgcttcc gatacggcgc
     3361 cggtgatgcg cgccttcgcc ggctacccgc aacgcgacaa ggtgctgatg gacggcgaca
     3421 cccgctccac catcaccctg ggctccggcc tgcaggtcga cctccgcgcc gtgccgccgg
     3481 agtgctacgg cgccgccctc gtctacttca ccggctccaa ggagcacaac gtcaagctcc
     3541 gccgccgcgg cgtcgaacgc gggctgcgca tctccgagta cggcgttttt cgggtcggcg
     3601 gggcggagaa gggagaccag gagggggtct tccacggcga gcggatcgcc ggcgccaccg
     3661 aggaggaggt ctacgccagc gtgggcctcg cctggatccc ccccgagctg cgcgaggacc
     3721 ggggggagat cgaggccgcg gcggccggcc ggctcccccg cctgctcgaa gccggagacc
     3781 tgcgcggcga cctccacatg cactcgacct ggtccgacgg ccgcaacagc atcgaggaga
     3841 tggtggaggc ctgcgccgcc cgcggctacg agtacatggt catctccgac cacagcaagg
     3901 cgctctccat ggtcaacggc ctcgacgcct accgcctcaa gctgcaatgg aaggagatcg
     3961 acgaggtcca ggcacgccac cccgagatcc gcatcctcaa ggcgatggag atcgacatcc
     4021 tggccgacgg ctcgctcgac ctggaggacg agatgctggc cggcctcgac ctcgtcctcg
     4081 tctccctcca ctccggcctc gggatgcccg gcgacgagca gacggcgcgc gtgctcaaag
     4141 ctctggagca cccggaggtc aacgtcttct gtcacccgac ggcgcggctg atcaaccgcc
     4201 ggcggccggt ggagatggac atggaccggg tgctctcccg cgccgccgag ctcggtgtgg
     4261 cggtggagat caactgcaac ccccaccggc tcgacctcaa ggacacccat gcccggctcg
     4321 ccaaggagct gggctgcaag ctggtggtga gcaccgactc ccaccgcacc cgcgagctgg
     4381 acctggtgcg ctacggcgtc gagcaggccc gccgcgccgg cctcgagccc ggggacgtcc
     4441 tcaatacgct gccgttgaag aagctcctca aggcggtgga gcgaaaaagt gctgtccggg
     4501 tctaaacttt ctctgttgct tgtccgtata aatagtaggg atgaaccttc acgcgaggtg
     4561 tctatgaaac aaccccagct tagcggcgtc tcacgggcgc tcgccctggc agggctcgcc
     4621 atggccctgc tggcagggcc cgcaaccagc caggacctcc tcaagtcgtt cgaggagagg
     4681 accacggtcc ataccctgaa gaacgggtgg accttcctca tcgtcgagcg gccggtggtg
     4741 ccggtcttct cgttctgcac cctggccgac gtggggtcgg cgcaggaggt gcccggcatc
     4801 acgggcctcg cccacatgtt cgagcacatg gcgttcaaag gttcgccgaa cctcggcacg
     4861 cgcgacgccg cgggtgagaa gaaggcgctc gaggagctcg aggccgccta ccaggcctac
     4921 aaccgcgagc gcctggccgc gcggccggac agcgcgaagg tcgagcggct cctcgccgcg
     4981 ttccgcgaca agcagaaggc ggcggcggcc ttcgtcgtcc agaacgagtt cgacgacgtc
     5041 atcacccgcg agggcggggc ggagctgaac gccaccaccg acacggacaa gacgcggtac
     5101 ttcttctcgc tcccctcgaa caagacggag ctcttcgcct tcctggagtc ggagcgcttc
     5161 ctccacccgg tcttccgcga gttctacgag gagcgggacg tggtccagga ggagcgccgg
     5221 gcgtccgtcg agagccaggc cgtcggccgg atgatggagc agttcaccgc cgccgccttc
     5281 tccgcccacc cctacggcaa cgacatcggc ggccacatga gcgacctcca gtcgttcacc
     5341 atcaccgacg ccgaggcgtt cttccgcacc tactacgcgc cggagaacct gctcacggtg
     5401 gtggtcggcg acgtgcgggc ccaggagttg atccccctgg tcgagaggta cttcggccgc
     5461 atcccggcgc ggcccggccc ggcgccgctg cgcaccgtcg agccgccgca gatcgtcgag
     5521 aagaccgtgg tcctggaaga cccccaggcc cagcccttct acatggaggg ctacaagaag
     5581 ccggcgaaca cccatcccga ccagccggtc tacgacgcca tcgacgacat tctcaccggc
     5641 ggccgcacgt cgcgcctcta ccgcgccctg gtgcgggaca agaagctcgc ggcgaacgtc
     5701 agcggctaca ccggtttccc cggcaccaag taccccaacc tgattacgtt cttcgtcacg
     5761 cccgccttcg gcgtgcagaa cgagcaggtg caggccgctc tgcgcgacga gctcgagcgt
     5821 ctgaagcggg aggacgtgag cgacgcggag ctcgcccggt acaagacgcg ggccaaggcc
     5881 cagctgttgc gccagttgcg caccaacgtc ggcatcgcca tcgagttcgc ggagtaccag
     5941 aacgaatacg gcgactggcg cgagctgttc cgctccctgg accgcctcga gaaggtgacc
     6001 aaggaggaca tccgccgggt cgccaaccag gttttccaga agtccagccg caccgtcggc
     6061 atggtcgtgc acaaacccca ggagacgccg ggctcccccc agagcagtcc ggccggcgcc
     6121 gcccgaaccg gcggcggagc ccgctaggag gaccgccatg aagctcattc ggaatcacgc
     6181 tctcgccgcc ctctgcgtcc tcgccctcct tctggtcctg ccggccggag ccggcgccga
     6241 cgcccaggtc gccacccgcg tcgaccagct gaagtacccg cccctgcggc ccttcgagat
     6301 cccgcagccg cagcgcgtgg tgctcgacaa cggcatggtg gtcatgctcc tcgaggaccg
     6361 cgagctgccg ctggtcgagg tgacggcgct ggtccgctcc ggcgcccggc tcgaccccgc
     6421 ggacaaggtc ggcctctcca gcctggccag caacgtcctg cgcatcggcg gcacgaccag
     6481 gatgccgagc gaccagctcg acgactggct ggaggaccgg gccgccacca tcgaggcggg
     6541 gacggccgac tccctcaccc gcgtcttcat gacttcgctg agccaggact tccccgaggt
     6601 gctgcgcgtc ttcggcgacg tgctgcgccg gccggccttc gccgccgacc gtctggaagt
     6661 ggcgcgcaac caggcgatca ccgcggtctc ccggcagaac gagaacccca tgggcatcgc
     6721 cttccgggag ttcaacaagg tggtctacgg cgccgactct cctctggctt ggaccccgac
     6781 ccacgccacc ctgcgcgccg tcagccgcga cgacatcgtc gcctggcacc gccggtactt
     6841 ccacgccgac cgggtcgtcc tcggcctggt gggggacttc cgctccgagg aggcgctgac
     6901 cctggtgcgc caggtcttcg gcgactggcc gcgcggcccg gccgaggcgc cgacggagac
     6961 gccgtaccgc aagcagccga gccccggcgt cttcttcgtc gagaagagcg acgtgccgca
     7021 gtcgcaggtg gtcatggggc acctcggcgt gcgccgcgac gaccccgact actatgccct
     7081 cgaggtggcg aaccaggtgc tctcgggctc cttcgcctcg cgcctgttct ccaacgtccg
     7141 tacccgcaag gggctggcct acaccgtctt cggcggggtc ttcagcgctt tcgacttccc
     7201 cgccaccacc ctgctctaca tgagcaccaa aaaggagacc accggcgcgg gcatcgactc
     7261 cctgctcaag gaggcgcgcg acgtcgtccg cgacctcccc cccaccgacg aggaggtcgc
     7321 caaggcgaag caggggctcc tcaactcctt cgtcttccgt tccgactcgc cgcgccgcgt
     7381 cctcgcgtcg ctcctgaact acgaggcgta cggctacccg ctggactggc tgacgcgcta
     7441 cccgaagggc atcgaggcgg tgaccgcgga gcaggtccgc caggcggcgg ctcgccacct
     7501 gcggcccgag gagttcgcca tcctggtggt cggccccgcc gaggggcaag accggccgct
     7561 caccgatttc ggcaaggtga cgccggtcga gctcacggct ccgggagcgc cgccggcgcg
     7621 gccggcaccg acccgcaagt agcgtcgccc cagcatccat accgcgaggc ccggctttcc
     7681 acgagccggg cctctttttc gacgttttcc agctttccct ctgcgggtgt gtagaaatta
     7741 caggcccggc gtatcccctt ccctccagag tggacctttt caagggtact tacctttcgt
     7801 atacctctgg cacgtaagtt gctgttaccg cagtgcagaa cgggcagtat gcacccgcat
     7861 catgggcgca ctttgggttg gcaccagtcc cggcctgcag cagactgcac gcccaggttc
     7921 ataccagccc gaaaagagga aggcggaccg gcagacggag gacgcggctg ccggcggcgg
     7981 gatgagacta cggcgtccac cacccctccg gcccgcctct tctgaagcgg tacagcaaga
     8041 tctacccatc ttggaagagg agactgagcg catgaaagga cgggtcttga tcaccggggg
     8101 cgccggcttc atcggctccc acctggcgga cgagctgctg gagcacggct accgggtgcg
     8161 ggcgttggac gacctctcgg aacaggtgca cgggccggcg gcggagcgtc cgggttatct
     8221 ccacccggat gtcgagctgg tgcgcggcga cgtgcgcgac ccgcgggtgg tgcgtgccgc
     8281 tctggagggc gtcgacgcgg tctatcactt cgccgcggtg gtcggcgtcg gccagagcat
     8341 gtatgagctg gagcgctaca ccagcgtcaa caacgtcggg acggcggtgc tgctcgaggc
     8401 gctgatcgag aaaccggtgg ggcggctggt ggtggcctcc agcatgagca tctacggcga
     8461 ggggctgtac cgcaacgcgc agggcgggct ggtggccggg accgagcgcg accgcgagca
     8521 gctcaaggcg cacgactggg agctgcgcga cgagcgcggc gaggccctgg cgcccctccc
     8581 cactccggag accaaggcgc ccaccctctc ctctctctat gccctgtcca agtacgacca
     8641 ggagcgcatg tgcctgatga tcggccgggc ctatgacatt ccggcggtgg cgctacgctt
     8701 cttcaacgtc tacggcacgc gccaggcgct gtcgaacccc tacaccggag tgctggcgat
     8761 cttcgcctcc cgcctgctga acggcaaccc gccgctgatc ttcgaggacg gccgccagat
     8821 gcgcgacttc gtgcacgtct ccgacgtcgc ccgcgcctgc cggctggccc tggaggtgcc
     8881 cgaggcccca tcggtggatg gccaggtgct caacgtcggc agcggccgcc agtacacggt
     8941 tctggagatc gcccgcagca tggccgaggt gctcggccgc gaggacatcg agccggagat
     9001 caccggcaag taccgggtgg gcgacatccg ccactgcttc gccgacatct cgctggcgcg
     9061 acggctcctg gggtacgagc cgcagatgcc gttcgagcag gggctgatcg agctcgcctc
     9121 ctggctcgag ggacaggtgg cctacgaccg tgtggcggaa gcccgcgccg agctggccgc
     9181 ccgggggttg acggtatgag caagccgagc acgaacacca agaaaatgcc tcccatcggc
     9241 ggtaccggcg gcaacggcag cagcaagatg gcgcgcgaga aggtcgtcct cgtcaccggc
     9301 ggatcgggct tcatcggcac caacctggcc gaccgcctag cctcctccgg ccggcgggtg
     9361 cggctgctcg acaacctgtc ccgccgcggc gtcgaggaga acctgcgctg gctcaagaag
     9421 acccacggcg accgcatctc gatcgaggtg gccgacgtgc gcgacccgtt cgccgtgcgc
     9481 cgcgctatgg ccggcgtcga gacggtcttc cacttcgcgg cccaggtggc ggtgaccacc
     9541 agcctgctcg acccggtaca cgacttcgag gtcaacgccc gcggcaccct caacgtcctc
     9601 gaggcggcac gcgcccagga ggagccgccg cgggtgctct tcacctccac caacaaggtc
     9661 tacggcggca tggaggatgt ggcgctgcgc ctcgaggggc ggcgctacga gccgtgcagc
     9721 ccggcggtgc gggcgcaggg gttctccgag gcgcgccccc tcgacttcca cagcccgtac
     9781 ggcgcctcca agggggccgc cgaccagtac gtggccgact acgcccgcag ctacggcctg
     9841 cgtaccgtgg tcttccgcat gagctgcatc tacgggccac accagttcgg caacgaggac
     9901 caggggtggg tggcccattt cctgatccgc gccatggagg accagccgat caccctctac
     9961 ggcgacggca tgcaggtgcg cgacgtcctc tacgtcgacg acctggtccg cgccttcctg
    10021 ctcgccgaga ggaacatcga ggccctctcg gggcaggcgt tcaacatggg cggcggcccg
    10081 gccaacaccg tctcgctgct ggagctgatc gacctcatcc atgagctcca ggggcggcgg
    10141 ccggaggtct tcttcgagga ctggcgcacc ggcgaccagc ggtactacgt ctccgacatc
    10201 cgcaagttcg aggcggccac gggctggagc ccgcttgtaa gcgcgcggca gggcctaggc
    10261 aacctttaca actggctcat ggagtcccgg ggcctcggcg agccacgtct ggtggcggct
    10321 agcggcagcc cggaaagcct ggcgctctga taaacgcttc gatccattca taaacctctc
    10381 tgcggccggg gagccccggc cgcggagagc ggagcgccgg caagcgggca cggatcttgg
    10441 atgtatgcga ccggaacggc agcttccatc cggtgccgca cacgagaggt gaaccggcat
    10501 gactgagagc agcaatcccc tgccccgcaa ggtcctcatg accgcggata cggtgggagg
    10561 cgtctggacg tacgcgctgg agctggcccg cggcctgggt gagcgggggg tcgaggtggc
    10621 gctcgccgcc atgggcggcc cgctgagcga cctgcaccgc gagaaggccg accgcatccc
    10681 ccgtctcaag gtgttcgaga gcacgttcaa gctggaatgg atggaggatt gctggcgcga
    10741 cgtcgagaag gccggcgagt ggctcctccg gctggaggac cgcatctgcc ccgacctggt
    10801 gcatctgaac ggctatgccc acgcggcgct cccctggaag gcgccgaaga tggtggtcgg
    10861 ccactcgtgc gtgctctcct ggtggcaggc ggtcaagggc gaggcggcgc cggagagctg
    10921 ggaccgctac cgcgccgagg tgaccgccgg gctcgccgcc gccgacctgg tggccgcccc
    10981 caccgcc
//
LOCUS       DASPJI010000166         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_9766, whole genome shotgun sequence.
ACCESSION   DASPJI010000166 DASPJI010000000
VERSION     DASPJI010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9766"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VEL74_04405"
     CDS             <1..279
                     /locus_tag="VEL74_04405"
                     /inference="COORDINATES: protein motif:HMM:NF018425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HYG61799.1"
                     /translation="VWTFTRTFYVLTATDLLIRSGPFRWSIPLAHVRQVLPTRNPLSS
                     PALSLDRLAIRHASGTIMISPEDQRRFLEDLVERTPGLVLDGTRAIRP"
     gene            complement(244..1095)
                     /locus_tag="VEL74_04410"
     CDS             complement(244..1095)
                     /locus_tag="VEL74_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017307842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HYG61800.1"
                     /translation="MLEPPAPEAVERERFEILQQLEDWLEVPLLILGFAWLALLVVEL
                     TYGLTPLLETASTVIWIVFLVDFGAKLLLAPRKLRYLRTNWLTALALLVPALRVLRFA
                     RVLRLTRAVRGVRLFRMLTSLNRGMRALRASLRRRGVAYVTLLTLLVALVGAAGMYAF
                     ESPAEGGRIESYGEALWWTAMLLTTLGSEEWPQTGAGRTLCFALALYAFAVFGYLTAA
                     LASYFVDRDAEAADSAVAGEQALRALHAEVAALRAEIRALGPRPRFPEGPGEEGLRAD
                     GASAVED"
     gene            complement(1126..>2178)
                     /locus_tag="VEL74_04415"
     CDS             complement(1126..>2178)
                     /locus_tag="VEL74_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HYG61801.1"
                     /translation="DRLAMVVEMEVLEDGTVAASDVYRALVHNHAKLVYEQVAAWLDG
                     DGPLPERAAAVPRLSDLLRLQDATARGLRRRRHERGALRLDRPEVTAEVVDGCTVTGF
                     REDRQNRARELIEDFMIAANETTARFLESRGVPSLRRVVRAPGRWPRIVDLARQLGED
                     LPPEPDSPALAAFLDGQREADPAGFPELSLAVTKLMGSGEYAVDRPGEESPGHFGLAV
                     EDYTHATAPNRRYPDLITQRQLKAVLDGGRSVYTEEELEELARHCTLREDEAAKVERQ
                     ITKSAAALLLQPRLGEEFEAVVTGASEKGTWVRLCGFPAEGRLVRGTRGADVGDRFRV
                     RLTEVDVERGYIDFER"
BASE COUNT          313 a          800 c          768 g          297 t
ORIGIN      
        1 gtctggacgt tcacccgcac cttctacgtc ctgaccgcga ccgatctcct catccggtcg
       61 ggccccttcc ggtggtccat cccgttggct catgtccggc aggtgctgcc cacgaggaat
      121 ccgctgtcca gcccggcgct ctccctggac cgtctggcga tccgtcacgc ctccgggacc
      181 atcatgatct caccggagga ccagcggcgc ttcctcgaag acctggtaga gcggacgccg
      241 gggttagtcc tcgacggcac tcgcgccatc cgcccgtaaa ccttcctctc ccggcccctc
      301 cgggaaccgt ggccgggggc cgagcgcccg gatctcggcg cgcagggcgg ccacctcggc
      361 gtggagggcc cgcagcgctt gttctcccgc gaccgccgaa tccgcggcct ccgcgtcgcg
      421 gtcgacgaag taggaggcca gggcggcggt caggtagccg aacaccgcga aggcgtacag
      481 ggcgagggcg aagcagagcg tcctgccggc gccggtctgc ggccactcct cggagcccag
      541 agtggtcaag agcatcgcgg tccaccacag ggcttcgccg tagctctcga tccgtccccc
      601 ctcggccggg ctctcgaagg cgtacatccc cgccgcgccg accagcgcca ccagcagggt
      661 gagcagggtg acgtacgcga ctccgcgccg gcgcaggctc gcccgcagcg cccgcatgcc
      721 ccggttgaga gaggtcagca tgcggaacag gcgcacgccc cgcaccgccc gggtgagacg
      781 gagcacgcgc gcgaacctca gcacccgcag ggccggcacc aggagggcca gggccgtcag
      841 ccagttggtg cggagatacc ggagcttgcg cggcgccagg aggagcttgg cgccgaaatc
      901 gacgaggaag acgatccaga tgacggtgct cgccgtctcg agcagcggcg ttagcccgta
      961 ggtgagctcg acgaccagca gggccagcca ggcgaagccg agaatgagga gggggacctc
     1021 cagccagtcc tcgagctgct gcaggatctc gaaccgctca cgctcgaccg cttccggcgc
     1081 agggggctcc aacatttggg atcacctccg ccgtccggcc gcccctcacc gctcgaagtc
     1141 gatatatccc cgctccacgt ccacctccgt gagccggacg cggaagcggt cgccgacgtc
     1201 ggcgccgcgg gtgccgcgga cgaggcggcc ttcggcgggg aagccgcaga ggcggaccca
     1261 ggtgcccttt tcggaggcgc cggtcaccac ggcttcgaac tcctcgccga gccgcggctg
     1321 gagcagcagg gccgccgccg atttggtgat ctggcgctcg accttggcgg cttcgtcctc
     1381 ccgcaaggta cagtggcggg cgagctcctc caactcctct tcggtgtaga cggagcgccc
     1441 gccgtccagc acggccttga gctgccgctg ggtgatgagg tcgggatagc ggcggttggg
     1501 ggccgtggcg tgggtgtagt cctccaccgc gaggccgaag tggccggggg attcctcgcc
     1561 gggacggtcc acggcgtact cgcccgagcc catcagcttg gtgacggcga gcgacagctc
     1621 ggggaaccct gcggggtcgg cctcccgctg tccgtccagg aaggccgcca gcgcgggcga
     1681 gtcgggctcc ggcggcagat cctcgccgag ctggcgggcg aggtcgacga tacgcggcca
     1741 gcggcccggc gcccgcacga cgcgccgcag cgacggcacc ccccgcgact ccaggaagcg
     1801 ggcggtggtc tcgttggcgg cgatcatgaa gtcttcgatc agctcgcggg cgcggttctg
     1861 gcggtcctcc cggaagccgg tcaccgtgca gccgtcgacg acctcggccg tgacctcggg
     1921 gcggtcgagc cgcagcgcgc cccgttcgtg gcgccgccgc cgcaggccgc gggcggtggc
     1981 gtcctggaga cggaggaggt ccgacagccg cggcaccgcg gccgcccgct ccggcagcgg
     2041 cccgtcgccg tccagccagg cggccacctg ctcgtagacc agcttggcat ggttgtggac
     2101 cagcgcccgg tagacgtccg aggccgccac cgtcccgtcc tccaggacct ccatttccac
     2161 caccatggct agccggtc
//
LOCUS       DASPJI010000167         6760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_583085, whole genome shotgun sequence.
ACCESSION   DASPJI010000167 DASPJI010000000
VERSION     DASPJI010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583085"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VEL74_04420"
     CDS             complement(<1..863)
                     /locus_tag="VEL74_04420"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="catalyzes the 4-phospho-L-aspartate from
                     L-aspartate and the formation of lysine from
                     meso-2,6-diaminoheptanedioate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HYG61802.1"
                     /translation="MSDSPTRWVVLKLSTTGIAHPERWRPLAEVVRRRIQEGLRPLVV
                     LPSLPRMPDLLWSFLGEAADGGGLRELVDDITEQHLELAEGLGFGREETEALLRPDLQ
                     ELWRLAVFAGVHHRPEEEASLSAEIMTVGELLLTRLAAAWLNRLGLPAAWHDARTSLT
                     VPHEEATTRTPRLTVSDPSPDEGLIRRFEALPRVVVTQAGIAGVAGSAGKGGETGTVR
                     FGHGGADFSAACFAAKLRAARCEIWTDGPGLFTADPSLLPSARLLRVLSHEEAQEILT
                     TGGRSPHPRAIP"
     gene            complement(900..1895)
                     /locus_tag="VEL74_04425"
     CDS             complement(900..1895)
                     /locus_tag="VEL74_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HYG61803.1"
                     /translation="MAKIWVVLQLRDGQVSRISWEAVAAGQHLATELGGSAKDVEAVV
                     LGSGSGLAAADVAQADLGAVHVADNPVLERYTPGAWIGALAPAIRAAAPDLVVFPHTY
                     QTVDFMPRLAQELDAGLLPEVTKVEKGDGGLVWTRPILGGKLQSKVRVKRQGTVLVSV
                     QSGAFPADRVARAGGGPEPRPLDADLATVQPDREILGYEEVGGDQVDLTKADVIVAVG
                     RGVGGADKMGPVEELARALGAEIAASRPVIDNGWLPRDRQIGSSGQTVAPRLYLAAGI
                     SGAIQHLVGMKGSSVIVAINKDPGAPIFNVAHYGIVGDLHEVLPALTEAVKAAKG"
     gene            complement(1895..2689)
                     /locus_tag="VEL74_04430"
     CDS             complement(1895..2689)
                     /locus_tag="VEL74_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HYG61804.1"
                     /translation="MRIAVCIKQVPNTEARLRVNREGKWVDEEDLAFVINENDEYALE
                     EGLQLAEKTGGEVVVFTLGPERAKEALRKALAVGAARAVHLSDPAFLGGDAMAAGRAL
                     AAAIKREGFDVVLTGSQSDDARFGGTGSVIAGALGWPHVWLVMGVELEDGNQSVKVTR
                     EMESGVNELSRVKLPAVFEIQAGINHPRYASLKGIMAAKKKEIAAVTPADLGLDAAQV
                     GAAGSWLEVVSVSFPDSGAGAQILEGDAKAAANALVQKLRNEARVL"
     gene            complement(2744..3250)
                     /locus_tag="VEL74_04435"
     CDS             complement(2744..3250)
                     /locus_tag="VEL74_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HYG61805.1"
                     /translation="MSQSLRCHVYYDSTLHPAPPSECGERVDLAAEPAAAARLERLGV
                     SPAVDLPAVLLTEPDGKLRILGLRLNDEETARLATGEAFPASQLTVFSSSWCPDCRRA
                     KRVLEEADATFAEVDVDQDVRAEALILERSGGRRVVPTLQFDDRLWAFNPAPPLLRRL
                     LGGAVVKA"
     gene            complement(3247..3945)
                     /locus_tag="VEL74_04440"
     CDS             complement(3247..3945)
                     /locus_tag="VEL74_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG61806.1"
                     /translation="MRGRVLITGAAGALGRSVVARFIDAGERVLAVDASEAALAELTQ
                     LAPTETLAVRRADLTSTPEVARLFDEAEQASGIFPAAVHLVGGFRWARFAEMSDQDWD
                     YLMKLNLETTFRVFRECARRFERAGGGAVVAVSSPAALLGETGVGGYAGAKAGVLRMV
                     ESLAREMAPFGGRANAILPGTMDTPANRAAMPDADPSRWVTTEAVADVIHFLTTPAAA
                     AINGAAIRVPGPTL"
     gene            complement(4448..4957)
                     /locus_tag="VEL74_04445"
     CDS             complement(4448..4957)
                     /locus_tag="VEL74_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018765971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG61807.1"
                     /translation="MTILETGRLLLRHAHDGDAEFFLELLNDPSFVENIGDRGVRTPE
                     QARRYIQERLVASYERHGFGMYVVERKESPGPIGICGLVRRDTLPDADVGFAFLPRYW
                     SKGYAVESAAAVMTYGREALGLGRIVAVVNPANQGSIRVLEKLGLRLEGMIRMPGEAA
                     DIQLFGPEG"
     gene            complement(4954..5946)
                     /locus_tag="VEL74_04450"
     CDS             complement(4954..5946)
                     /locus_tag="VEL74_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HYG61808.1"
                     /translation="MTILKDDFSENIGPSRPVIILGVDRSGTSVVAQLTHEWGAYGGD
                     PSSLMRSDSANPHGFWEYLPMVRLIHQVFREVGYDFWRPGYEEALRTTAGDPRFRAPA
                     EELIASMAAGGAAWFWKEPWLCLQLPFWQEIWGDASYIITVRNPYDSSVSWQKMTLPS
                     GAESRLRLVALGLLRWQYFMLSVLRHVEGSRRLLFVQYEDLIQDPRAQSARICGFLDR
                     EVGTARVAEGRAERMAEAVDGSLWRNRSPVDFSQIEIASREQKALYELLRSKVADPEA
                     RFDPDSYPMYPGWREYLHNFNEAEGAVRQFLGSRLVRLALALDGWRASFRLRRS"
     gene            complement(5943..6098)
                     /locus_tag="VEL74_04455"
     CDS             complement(5943..6098)
                     /locus_tag="VEL74_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61809.1"
                     /translation="MSPIWKTVLLELLLHNLRHVQHHGGQLNLILCQQTGGDAPRWVA
                     RAKEVLP"
     gene            complement(6095..>6760)
                     /locus_tag="VEL74_04460"
     CDS             complement(6095..>6760)
                     /locus_tag="VEL74_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYG61810.1"
                     /translation="AVKEDYSYRGSFAVKPLAKDVYLFENVTETTGQWSYNVLVVVLG
                     DSVLVAEAPVSSAVSDSVLAKVREIAPGKPVRYLVQSHHHGDHLGGIRSYIAEGATIL
                     AGAGTAGLIEKIARAPFELAPDRLQKEPRAPKIEVVRGSRTIRSGGREVVIHDIGPSP
                     HARDMLFVHLPRERILYQADLVNEGEYPENETTRHFQARLKALGLEVKTLAGLHGKVR
                     TLN"
BASE COUNT          996 a         2404 c         2378 g          982 t
ORIGIN      
        1 gggatggcgc gcgggtgggg gctgcggccg ccggtggtga ggatctcctg cgcctcctcg
       61 tggctcagca cccgcagcag ccgcgccgac ggcagcagcg acgggtcggc ggtgaacagg
      121 ccggggccgt cggtccagat ctcgcaccgc gcggcgcgca gcttggcggc gaagcaggcg
      181 gccgagaagt cggcgccgcc atggccgaac cggaccgtgc cggtctcgcc tcccttcccg
      241 gcgctcccgg ccacccccgc gatcccggcc tgggtcacca ccacccgcgg cagcgcctcg
      301 aaccgccgga tcagcccctc gtccggcgac gggtcgctga cggtgagccg tggggtgcgg
      361 gtggtggcct cctcgtgcgg gacggtgagg gaggtgcggg cgtcgtgcca agcggccggg
      421 agccccagtc ggttgagcca ggcggcggcg aggcgggtga ggagcagctc ccccaccgtc
      481 atgatctcgg cgctgagcga ggcctcctcc tccggccggt ggtggacccc ggcgaacacg
      541 gcgaggcgcc acagctcctg gaggtccggc cggagcagcg cctcggtctc ctcgcggccg
      601 aagcccagcc cctcggccag ctcgaggtgc tgctcggtga tgtcgtcgac cagctcgcgc
      661 agccctcccc cgtccgcggc ctcgccgagg aagctccaga gcaggtccgg catgcggggg
      721 agggacggca ggacgaccag cggacggagc ccctcctgga tgcgccggcg gacgacctcg
      781 gcgaggggcc gccagcgctc gggatgggcg atgccggtgg tgctcagctt gagcaccacc
      841 cagcgcgtgg gagagtcgct caccgtctct cgccgtctct tgccgaggga gggagccggc
      901 taccccttgg ccgccttgac ggcttcggtc agcgccggca gcacctcgtg gaggtcgccg
      961 acgatgccat agtgggcgac gttgaagatg ggcgctccgg ggtccttgtt gatcgccacg
     1021 atcaccgagg agcccttcat ccccaccaga tgctggatgg cgcccgagat gcccgcggcg
     1081 agatagagcc ggggggcgac cgtctggccc gaggagccga tctggcggtc gcgcggcagc
     1141 cagccgttgt cgatcaccgg ccggctggcg gcgatctccg cccccagcgc cctggccagc
     1201 tcctcgaccg gccccatctt gtcggcgccg cccacaccgc ggccgacggc gacgatcacg
     1261 tccgccttgg tgaggtccac ctgatcgccg cccacctcct cgtagccgag gatctcgcgg
     1321 tccggctgca ccgtggcgag gtcggcgtcg agcggccggg gctcgggacc gccgccggcg
     1381 cgggcgaccc ggtcggccgg gaaggcgccc gactggacgg agaccagcac cgtcccctgg
     1441 cgcttgaccc gcaccttgga ctgcagcttg ccgccgagga tggggcgggt ccagacgagg
     1501 ccgccgtccc ccttctccac cttcgtaacc tcggggagga ggccggcgtc cagctcctgc
     1561 gccagccggg gcatgaagtc gaccgtctga taagtgtggg ggaagaccac caggtcgggc
     1621 gccgcggcgc ggatcgccgg cgccagcgcg ccgatccagg cgcccggcgt gtagcgctcg
     1681 agcaccgggt tgtcggccac gtgcacggcc ccgaggtcgg cctgggccac gtcggcggcg
     1741 gcgaggccgg aaccggagcc gagcaccacg gcttccacgt ccttcgcgga gccgcccagc
     1801 tcggtggcga ggtgctggcc ggcggcgacc gcctcccagg agatgcgcga cacctggcca
     1861 tcgcgcagtt gcaggaccac ccagatcttc gccatcagag caccctcgct tcgttccgga
     1921 gtttctggac cagcgcgttg gcggcggcct tggcgtcccc ctcgaggatc tgggcgccgg
     1981 cgccgctgtc ggggaacgac accgagacca cctcgagcca ggagcccgcg gcgcccacct
     2041 gcgcggcgtc gagccccagg tcggcggggg tgacggcggc gatctccttc ttcttggccg
     2101 ccatgatccc cttgagcgag gcgtagcgcg ggtggttgat gcccgcctgg atctcgaaca
     2161 cggcggggag cttcacccgc gagagttcgt tgacaccgct ctccatctcg cgggtcacct
     2221 tgacgctctg gttgccgtcc tccagctcga cgcccatgac cagccagacg tgcggccagc
     2281 cgagggcgcc ggcgatcacc gagccggtgc cgccaaagcg ggcgtcgtcc gactgcgagc
     2341 cggtgagcac cacgtcgaag ccctcgcgct tgattgcggc ggcgagggcg cggccggcgg
     2401 ccatggcgtc gccgcccagg aaggccgggt ccgacaggtg gacggcgcgc gccgcgccga
     2461 cggccagggc cttgcgcagc gcctccttgg cgcgctccgg cccaagggtg aacaccacca
     2521 cctctccgcc ggtcttctcg gcgagctgga ggccctcctc cagcgcgtac tcgtcgttct
     2581 cgttgatgac gaaagcgagg tcctcctcat cgacccactt gccttcccgg ttcacccgga
     2641 gccgggcctc ggtgttgggg acctgcttga tgcagaccgc tatcctcatg aaggtctttc
     2701 ctcttctcgt atggattgga cggagtgcct cacgccggcc gtctcaggcc ttcaccaccg
     2761 cgccgcccag caggcggcgc agcagcggcg gcgcggggtt gaaggcccac agccggtcgt
     2821 cgaactggag cgtggggacg acccggcggc cgccgctgcg ctccaggatc agggcctcgg
     2881 cgcggacgtc ctggtcgacg tcgacctccg cgaaggtggc gtcggcctcc tcgagcaccc
     2941 gcttggcgcg ccggcagtcc gggcaccagg acgagctgaa gacggtgagc tgcgacgccg
     3001 ggaaggcctc gccggtggcg agccgcgccg tctcctcgtc gttaaggcgc agccccagga
     3061 tgcggagctt gccgtccggc tcggtgagca gcaccgccgg cagatcgacc gcgggcgaga
     3121 cgcccaggcg ctcgagcctc gccgccgccg ccggctcggc ggcgaggtcg acgcgctcgc
     3181 cgcactcgga cggcggcgcg gggtgaaggg tggagtcgta gtacacgtga caacggagcg
     3241 attggctcat agggttggtc ccggaacccg gatggcggcg ccgttgatgg ccgcggccgc
     3301 gggcgtagtg agaaagtgaa tgacgtccgc gacggcctcg gtggtgaccc agcgggaggg
     3361 atcggcatcg ggcatggccg cgcggttggc cggggtgtcc atcgtgcccg gcaggatggc
     3421 gttggcgcgt cccccgaagg gagccatctc gcgcgccagg gactccacca tgcggaggac
     3481 gccggccttg gcgccggcat agccgccgac gccggtctca ccgagcaggg cggcgggcga
     3541 cgagaccgcc accacggctc ccccgccggc gcgctcgaag cggcgggcac actcacggaa
     3601 cacgcggaag gtggtctcga ggttgagctt catcaggtag tcccagtcct ggtcggacat
     3661 ctcggcgaag cgcgcccagc ggaagccgcc caccaggtgg acggcggcgg ggaagatccc
     3721 cgacgcctgc tcggcctcgt cgaacagccg cgccacctcc ggagtggacg tcaggtcggc
     3781 ccgccggaca gcgagcgtct cggtgggcgc gagctgggta agctccgcca gggccgcctc
     3841 ggacgcgtcg acggcgagca cacgctcgcc cgcgtcgatg aaccgcgcca cgacgctccg
     3901 cccgagggcg cccgcggcgc ccgtgatgag gactctccct cgcatggttt tcgttcccgg
     3961 actaaaacag acaggcagcg cccgccactg ctatgagtgc cgcggcgggc gacacatgag
     4021 actaccacgg gtggcaagag gggcggtcag acggcgtagc cggccagccg ggccgcgggc
     4081 ggactggctt gcggtatcct ggtcaccccg gctggcaaag gcctctcggg gtttacgaga
     4141 cgccggagaa cggccctgac cggcgatgac ccgcgatccc caaaagggga tgcccctctc
     4201 ccttcaaggg gaagccctcc tcccctttcc gggagagagc cttcccgaaa gggaggggcc
     4261 tctccctcaa atgggaggac gccctccccg aaacggtttg gcgccttccc ttatccggta
     4321 cacccccttc acctttcggg tacatcccct ccccaacagg ggagccccct ctccctcaaa
     4381 ggtacgagcc tcgtcccggc ttttcctttc gttgccgcac ccccgcaagc ggctggctga
     4441 ggggccgtca accctccggc ccgaagagct ggatatccgc ggcctctccc ggcatccgga
     4501 tcatgccctc gagcctcagg ccgagcttct ccagcacccg gatggagcct tgattggccg
     4561 gattgaccac ggccacgatc cgcccgagcc cgagcgcctc ccgtccgtag gtcatcaccg
     4621 cggcggccga ctcgacggcg taccccttgg accagtacct cggcaggaag gcgaagccga
     4681 catcggcgtc cggcaacgtg tcccgccgca ccagcccgca gatcccgatc ggcccggggg
     4741 actccttccg ttccaccacg tacatgccga agccgtggcg ctcgtagctg gcgacgagcc
     4801 gctcctggat atagcgccgg gcctgctccg gcgtcctcac cccccggtcg ccgatgttct
     4861 cgacgaagga ggggtcgttc aacagctcga ggaagaactc cgcgtcgccg tcgtgcgcgt
     4921 ggcggaggag caagcggccg gtctcgagga tggtcatgac cggcgaaggc gaaaggaggc
     4981 gcgccacccg tccagggcga gagcgaggcg cacgagacgt gagccgagga actgccggac
     5041 ggcgccctcg gcttcgttga agttgtggag gtactccctc cagccggggt acatgggata
     5101 ggagtcggga tcgaacctcg cctccgggtc ggctactttg ctccgcagca gctcgtaaag
     5161 cgccttctgc tcgcgcgagg cgatctcgat ctgggagaaa tcgaccgggc tccggttccg
     5221 ccagaggctg ccgtcgacag cctccgccat acgctcggcc cggccttcgg caacgcgcgc
     5281 ggtccccacc tcccggtcga ggaacccgca gatccgcgcg ctctgggcgc gaggatcctg
     5341 gatgaggtcc tcgtactgga cgaagagcag gcgtcgcgag ccttcgacgt ggcggaggac
     5401 ggagagcatg aagtactgcc agcgcagcag gcccagggcg acgaggcgga gccggctctc
     5461 ggcgccggac ggcagcgtca tcttctgcca cgacacggaa gagtcatagg ggttccgcac
     5521 ggtgatgatg tagctggcgt cgccccagat ctcttgccag aacggcagtt gcaggcagag
     5581 ccacggctcc ttccagaacc aggccgctcc ccccgcggcc atggaggcga tgagctcctc
     5641 cgcgggcgcg cggaaacgcg ggtccccggc cgtggtccgc aaagcctcct cgtagccggg
     5701 ccgccagaag tcgtagccga cctctctgaa aacctggtgg atcagccgca ccatggggag
     5761 gtactcccag aacccgtgcg ggttggccga atcgcttcgc atcagggaag atggatcgcc
     5821 gccgtaggca ccccactcgt gggtgagctg cgcgacgacc gaagtgccgc tacggtccac
     5881 cccgaggatg atgacgggac ggctggggcc gatgttctcg ctgaaatcgt ccttcaggat
     5941 cgtcatggaa gaacctcttt tgcacgggcc acccagcgtg gggcatcgcc accggtctgc
     6001 tggcacagga tcaggttgag ctgcccgccg tggtgctgga catgacgcag gttatgcagg
     6061 agcagctcca ggaggacggt tttccagatc gggctcaatt cagcgtccgc acctttccat
     6121 gtaacccggc gagggtcttg acctccaggc cgagggcttt cagccgggcc tggaagtgcc
     6181 gggtggtctc gttctcggga tactccccct cgttcaccag gtccgcctga tacaggatcc
     6241 gctcccgggg gagatggacg aagagcatgt ccctggcgtg cgggctcggg ccgatgtcat
     6301 ggatcacgac ctcccggccg ccggagcgga tcgtgcggct cccccgcacg acttcgatct
     6361 tcggcgcccg cggctccttc tggaggcggt ccggcgccag ctcgaacggt gccctggcga
     6421 tcttctcgat caggcccgcc gtgccggcgc ccgccaggat ggtggccccc tcggcgatgt
     6481 aggaccggat cccgcccagg tgatcgccgt gatgatggct ctggaccagg tagcggaccg
     6541 gcttcccggg ggcgatctcg cggaccttcg ccagcacgct gtccgacacc gcgctgctga
     6601 cgggcgcctc ggccaccagg acggagtcgc cgaggaccac caccagcacg ttgtaggacc
     6661 actgccccgt cgtctcggtg acgttctcga agagatagac gtccttggcc agcggcttga
     6721 ccgcgaacga gccgcggtag gagtagtcct ccttgaccgc
//
LOCUS       DASPJI010000168         4762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_523995, whole genome shotgun sequence.
ACCESSION   DASPJI010000168 DASPJI010000000
VERSION     DASPJI010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523995"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VEL74_04465"
     CDS             complement(<1..821)
                     /locus_tag="VEL74_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG61811.1"
                     /translation="MKRQGDRPPRRPSLSVSLLLATILILPPAAPSQTTAPPAGPAAA
                     ESFSDTFDVNVVNVEVFVTDQHGQRVSGLTRDDFQILEDGKPVEITNFYAADVTVAAP
                     GPDQESQGPAAPVPMPEEQRLQLGVFVDTSNLSERPRKRLQQELTTFLSTQLSPEDRV
                     LLATYGKEGFKALPLRAGDPKAMKESVAEVFEGSPALSQSLMQLRSLIRQIERARSPF
                     SGNAGDAAEGQLDAARIADALDSYSKQLMTGAKGSVEALDRFLSGLAGLPGRKAVL"
     gene            complement(955..1137)
                     /locus_tag="VEL74_04470"
     CDS             complement(955..1137)
                     /locus_tag="VEL74_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61812.1"
                     /translation="MRKKLIYLTLAMAAFAAAQAGFVATVSAAPPGCDTYCCPNDPGR
                     CVTCCPGKPCPRLYCP"
     gene            1270..1605
                     /locus_tag="VEL74_04475"
     CDS             1270..1605
                     /locus_tag="VEL74_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006528893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYG61813.1"
                     /translation="MGEGDKPLVWLHGEVKTPPLSPRSRLQAGFLLRRLQQGETLSMP
                     DSRPMPSIGPSCHELRIDEANATWRIFYRIDPDAIVILDVIKKKARATPKAVIDICKR
                     RLSEYDRLV"
     gene            1615..1980
                     /locus_tag="VEL74_04480"
     CDS             1615..1980
                     /locus_tag="VEL74_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG61814.1"
                     /translation="MEKEKKARLEARGWKVGSTEDFLGLTPEESAYVDLRLKLSDAVR
                     ELRKEKRLTQVQLAERLHSSQSRIAKIEAADETVSLDLLIRSLLALGASSKDLAKVIA
                     GHRGDRYGANLLTSFSTPR"
     gene            2192..2734
                     /locus_tag="VEL74_04485"
     CDS             2192..2734
                     /locus_tag="VEL74_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61815.1"
                     /translation="MRSPLQGSTPGAPDRPSSNAFSESFLQSDRERNEPAEIDDPEGE
                     GPWLVLPDSSGGWPGWEEVHGLWPLGERPERGDPAAFVSGGRSLALCAAVARPVAGRD
                     PLYVLGQDRWRGGYPLLRQGRQEAWMGTFFPEWALITSALVSLGQRPEAVAVYLEALG
                     PSCRRRVGAILHERLSTEKS"
     gene            complement(2805..3887)
                     /locus_tag="VEL74_04490"
     CDS             complement(2805..3887)
                     /locus_tag="VEL74_04490"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HYG61816.1"
                     /translation="MKRLILCCDGTWNRADQEHEEVPCPSNVVKMAYRVAKRDGDTLQ
                     VTYYGQGVGTGNAVDRFSGGAFGRGLEENIHDAYRFLIANYEPGDEIYIFGFSRGAYT
                     ARSLGGMIRKCGILQRASVGEYHNALDLYRSAAVKADDPPALAFRERHSLCGKDPVAI
                     HLIGVWDTVGALGIPLRGLRWVTRKDYQFHDTELSQSVKFAYHALAIDEHRGPFEPTL
                     WMAKAKPGQTLEQVWFCGAHSDVGGGYPERGLSDLALDWMIAKAKGAGLAFDASAMAA
                     LPLHPDHREKVHDSKKGVYNLTRGLDRPIGLTSPGTGDPPGTSAKNDPTQSLHPSVLK
                     RWDEDSSYRPKSLREYFQRIGDPRGR"
     gene            4036..4758
                     /locus_tag="VEL74_04495"
     CDS             4036..4758
                     /locus_tag="VEL74_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013937286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYG61817.1"
                     /translation="MGRIFETRKHTMFARWDRMAKQFARIGKEITIAVKAGGPDPASN
                     PMLRRVVQNARSVNMPKDKVESAIKRAAGKEAESYEEIVYEGYAPHGIAVMIETATNN
                     PTRTVSNLRTHFNKGGGNLATAGAVTFMFKRMGVFRLNPAGLDAEELELELIDHGLEE
                     IGETTGEKGEPQLLLRCAFADFGTLQKALEDRGITPLSAESEYVAVTPAELSEDQATD
                     VLKLIDRLEQDDDVQKVFHNLA"
BASE COUNT          795 a         1517 c         1660 g          790 t
ORIGIN      
        1 agcaccgcct tgcggccggg caggccggcg agtcccgaga ggaaccggtc gagggcctcg
       61 accgatccct tcgcgccggt catcaactgc ttgctgtagg aatccagggc gtcggcgatc
      121 cgcgccgcgt cgagctgccc ctccgccgcg tcccccgcgt tccccgagaa gggggacctc
      181 gcccgctcga tctggcggat caggctgcgg agctgcatga ggctctgcga tagggcgggg
      241 gagccctcga agacctcggc cacggattcc ttcatggcct tcggatcgcc ggcgcggaga
      301 ggcagggcct tgaagccctc cttcccatag gtcgccagca ggacgcggtc ctcgggagag
      361 agctgggtgg agaggaaggt cgtcagctcc tgctgcagcc gcttgcgcgg cctctccgac
      421 aggttggagg tgtcgacgaa cacgccgagc tgcagccgct gctcttccgg catcggcacc
      481 ggcgcggcgg ggccctggct ctcctggtcc ggcccggggg cggcgaccgt gacgtccgcg
      541 gcatagaagt tggtgatctc gaccggcttg ccgtcctcca ggatctggaa gtcgtcgcgg
      601 gtcaggccgg agacgcgttg gccgtgctgg tcggtgacga agacctcgac gttgacgacg
      661 ttgacgtcga aggtgtcgga gaaggactcc gcggctgccg ggccagcggg aggagctgtt
      721 gtctgggacg gtgcggcagg aggaaggatc aggatcgtgg cgagaagaag agagacggac
      781 agggatgggc ggcgaggtgg acggtcgcct tggcgcttca taccggatct cctttaagcc
      841 aacatagatg gtctacattg tagacgtccc atgatgccgg aaatcctcac atctggctgg
      901 agtgtcccgg aagccgggga cctcagggtc cccggctcat ccggcgttcg agacctacgg
      961 gcagtagagg cgggggcagg gcttgcccgg gcagcaggtg acgcaccgtc cggggtcgtt
     1021 cgggcagcag taagtgtcgc atccgggcgg cgcggccgag accgtagcga cgaagccggc
     1081 ctgagccgcc gcgaacgccg ccatggccag tgtcaggtag atgagcttct tgcgcatgtc
     1141 agatcctcca gagagatggt ctataccctt gcaggtgccc cggggcttct ggggcgagcc
     1201 tgatgccccg ccgtcgagcc attcgtctac ggcttgacgg catgtcaaga ttgacatact
     1261 ctggtgagaa tgggagaggg ggacaagccg cttgtctggc tacatggtga ggtcaagact
     1321 ccgcccctgt cacctcggtc gcgcctgcaa gctggcttcc tcctgcggcg gctccagcag
     1381 ggcgagacgc tctcaatgcc ggattctcgc ccgatgcctt caataggtcc cagctgccat
     1441 gagctgcgta tcgacgaagc gaatgctacg tggcgcatct tctaccggat tgacccggat
     1501 gccattgtga tcctcgacgt tatcaagaaa aaggcacggg ctactccgaa ggccgtgatc
     1561 gatatctgta agcgccggct gtctgaatac gaccggctgg tctaggaggt ccagatggag
     1621 aaggagaaga aggcgcggct ggaggcgagg ggatggaagg ttggatcaac ggaggatttc
     1681 ctcgggctga cacccgaaga gtcggcctat gtggatctcc ggctcaagct cagtgacgct
     1741 gtgcgggagc ttcgtaagga gaagcgcctg acccaagttc aactcgctga gcgtcttcat
     1801 tcaagccaga gccggatcgc gaagatcgag gctgcggacg agactgtctc cctcgacctc
     1861 ctcattcgct ccctcctcgc tcttggggcc agcagcaagg acctggctaa ggtgattgcc
     1921 ggccatcgtg gggaccgtta tggagcgaac ctcttgacga gcttctcgac cccacgctag
     1981 agtacgagtg acgtaagcag agcgaagtca tcccggcagg tagcgaggag gtggtcgcgg
     2041 aggcagggaa gaaaacgagg taccccgcag caggttgaag cgaggcggcc ggcgcgtggc
     2101 cgaccgccaa gagtgacaat ctggttagcg gctcgaggtg agcggccagg ctacaccccg
     2161 agccttgacc acacagggag aggtaaactc catgcgtagc ccacttcagg gtagcacccc
     2221 tggtgcgccc gaccgtccgt cgtccaatgc tttctccgaa tccttccttc agtccgaccg
     2281 cgagcggaac gagcccgccg agatcgatga tcccgagggc gaaggcccct ggctcgtgct
     2341 cccggactcc tcgggcgggt ggcctggttg ggaggaggtc cacgggcttt ggcctctcgg
     2401 tgagcggccc gagcggggcg atccggcggc gttcgtctcc ggcgggcgct cccttgcgct
     2461 ctgcgccgcc gtcgcccggc ccgtcgccgg ccgcgatccc ctctacgttc tcggccagga
     2521 ccggtggcgc ggcgggtatc cgctgctccg gcaaggtagg caggaggcgt ggatggggac
     2581 cttcttcccg gaatgggcgc tgatcaccag cgctctcgtc agcctggggc agcggcccga
     2641 ggccgtcgcc gtctacctgg aggctctcgg gccttcctgc cggcggcggg tcggagccat
     2701 cctccacgag cgtctctcga ccgaaaagag ctgaaggcgt cgtatctaga taggagagag
     2761 gcggagggaa atccctccgc ctctcgcggc cgcttcccgt cggcctacct cccccgcggg
     2821 tcgccgatgc gctggaagta ctcgcggagc gacttcggcc ggtagctgga gtcctcgtcc
     2881 cagcgcttga ggacgctcgg gtggagcgat tgggtggggt cgttcttcgc ggaggtaccg
     2941 ggaggatcgc ccgtcccggg ggatgtcagg ccgatcgggc ggtcgagccc acgggtgagg
     3001 ttgtagacgc cctttttgga gtcgtggacc ttctcgcggt ggtcgggatg cagcggcagg
     3061 gccgccatgg cggaggcgtc gaaggcgagg cccgcgccct tggccttggc gatcatccag
     3121 tccagcgcca ggtccgagag gccccgctcg gggtagccgc cgccgacgtc gctgtgcgcg
     3181 ccacagaacc agacctgctc cagggtctgc cccggcttgg ccttcgccat ccagagcgtc
     3241 ggctcgaacg gcccccgatg ctcgtcgatg gccagggcgt gataggcgaa cttcacgctc
     3301 tggctcagct ccgtgtcgtg gaactggtag tccttgcggg tgacccagcg gaggccgcgc
     3361 agggggatcc ccagcgcccc aacggtgtcc cagacgccga tgaggtggat cgcgacgggg
     3421 tccttgccgc agagagagtg cctctcccgg aaggccaggg cgggcgggtc gtcggccttg
     3481 acggccgcgc tccggtagag gtccagggcg ttgtggtact cccctacgga tgcgcgctgg
     3541 aggatgccgc acttgcggat catccctccg aggctgcggg ccgtgtaggc accccggctg
     3601 aagccgaaga tgtagatctc gtcccccggc tcgtagttgg cgatcaggaa acggtaggcg
     3661 tcgtggatgt tctcctcgag cccgcggccg aaggcgccgc ccgagaagcg gtccaccgcg
     3721 ttgccggtgc cgaccccctg gccgtagtag gtcacctgga gggtgtcgcc gtcccgcttc
     3781 gccacccggt aggccatctt gaccacgttg gaggggcagg ggacctcctc gtgctcctga
     3841 tccgcccggt tccacgtccc gtcgcagcag aggatgagcc gcttcatcga tgcctccctt
     3901 caggtggcga ggttcttgaa aacctgcctg aatgctagcc gatcgcggtg tccggaggcc
     3961 agcggaggcc agcggaggcc agccgccggg cggggggctt tactcggcgg cgatgctcgg
     4021 gctagattca ccgccatggg acgcattttc gagaccagaa aacacacgat gttcgcccgc
     4081 tgggaccgga tggcgaagca gttcgcccgg atcggcaagg agatcaccat cgccgtcaag
     4141 gcgggtggcc ccgatccagc gagcaacccg atgctccgcc gggtcgtcca gaacgcgcgc
     4201 tcggtcaaca tgccgaagga caaggtggag tcggcgatca agcgggcggc gggcaaggag
     4261 gcggagagct acgaggagat cgtctacgag ggctacgctc cccacggcat cgccgtcatg
     4321 atcgagaccg cgacgaacaa cccgacccgc accgtctcca acctgcggac ccacttcaac
     4381 aaggggggcg gcaacctcgc caccgcgggc gccgtgacct tcatgttcaa gcgcatgggc
     4441 gtcttccggc tcaacccggc cggtctcgac gccgaggagc tcgagctcga gctcatcgac
     4501 cacggcctcg aggagatcgg cgagaccacg ggcgagaagg gcgagccgca gctcctgctc
     4561 cgttgcgcct tcgccgactt cggcaccctg cagaaggcgc tcgaggaccg cggcatcacc
     4621 ccgctctccg ccgagtcgga gtacgtcgcc gtgactcccg cggagctctc cgaggaccag
     4681 gccacggacg tcctgaagct gatcgaccgg ctcgagcagg acgacgacgt ccagaaggtc
     4741 ttccacaacc tcgcctgatc ga
//
LOCUS       DASPJI010000169         5156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_695508, whole genome shotgun sequence.
ACCESSION   DASPJI010000169 DASPJI010000000
VERSION     DASPJI010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695508"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VEL74_04500"
     CDS             complement(<1..217)
                     /locus_tag="VEL74_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG61818.1"
                     /translation="MQFSTSPRASGPGSRRILVVEDDPDLRALIERLLCDAGFDVSAA
                     ASGEQALDVIGQRGFPHLALVDIVLPGI"
     gene            402..731
                     /locus_tag="VEL74_04505"
     CDS             402..731
                     /locus_tag="VEL74_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HYG61819.1"
                     /translation="MSFDPEYQGFRTQFTDRLPARLEEIEAAWRQVREGGWSRESLAL
                     FHRLAHSLAGACAMFDLAAMAATARELEHLLKRLAVEPGEVSERRIESLLDRLRQAVP
                     PKHLALL"
     gene            846..1934
                     /locus_tag="VEL74_04510"
     CDS             846..1934
                     /locus_tag="VEL74_04510"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HYG61820.1"
                     /translation="MLAAPMLNRGRHSLFADSSTDYSTRAIDLVRRSAPIDMLGLLTL
                     DWNRLYQWQRQPASFGDGEIQKLTGTGTRVFHPAVDPNHPDPHGTTLRWISGWNRLLA
                     AHPERLVRIGSADDLERVREGSRLGLLIGFQNSEHFRDSADVALFHGLGQRVSQLTYN
                     VRNRLGSGCNQDGPLTPYGAGIVAAMNEVGMAVDISHCSDRTSFDTVAASRKPVLITH
                     SNCRALVPHKRCKPDSVIRAVAARGGVMGITVIPAFAGKRQPVTLDDVLDHFDYAARL
                     VGPEHVGLGSDCDTDAIDPKTGRIRPRYVVHGLRHVRRTFDIAEGLLRRGYAEKDVEL
                     ILGGSFRRALAEIWGPAPAAPTPVRAAG"
     gene            complement(1941..3713)
                     /locus_tag="VEL74_04515"
     CDS             complement(1941..3713)
                     /locus_tag="VEL74_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYG61821.1"
                     /translation="MAERIMDVLERTAGTHGARPAMKIKEGGTWRTTTWKEYRDQAHL
                     AARGFMKLGLQPGQGVAIMSFNRPEWFLADVGAVAAGGYPAGIYTTLAAEQCQYIADH
                     CEAAVVVVENRQYLDLFLSIRGQLPKLKALVLMTGDASGSGSDIYTWKQLLEMGAQVP
                     EADLRARIDAQKPQDLCTLIYTSGTTGPPKAVALSHHNVVWTAQRVVETYEVNADDQF
                     LSYLPLSHIAEQVVSFHGPMATGGCSWFAESLEKLGENLREVRPQFFFAVPRVWEKMQ
                     AAIQAAGAQNSGLKKKIAAWARGIGAETGRAEQEGRSRPLFYPLARKLVFSKVREKLG
                     LDRARICATSTAPISVETLQFFLSLDIPIMEVYGMSECTGPATFSTPKRYTPGKVGFP
                     LPGTEIKTAEDGEVLMRGPHVFLGYYKNEAATRETVDEQGWLHSGDIGELGGDGLLRI
                     TDRKKELLITSGGKNIGPQILESKLKQIRAVSQAVVVGDRRNYLAALLTLDPANAPAE
                     AAAAGSQARTLEEIAACPTFRAYLEKQVEQVNAGLARYETIKRFHVLPQELSIEGGEL
                     TPTMKLKRRVIYDKYAREIEGLYA"
     gene            complement(3770..4990)
                     /locus_tag="VEL74_04520"
     CDS             complement(3770..4990)
                     /locus_tag="VEL74_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013918852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MupA/Atu3671 family FMN-dependent
                     luciferase-like monooxygenase"
                     /protein_id="HYG61822.1"
                     /translation="MADDLSKRLAGLTPEQRELLLRRLRKEGLGVPGEAPVPAAPAPS
                     GHPLFTLDPGPEPEPSDRPVDFSLFFFSDGGAKSPAESYDLLLKSARFADRHGFAAVW
                     TPERHFQHFGGFYPNPSILGAAFAAVTERLGLRAGSVVLPLHDPLRIAEEWAVVDRLS
                     NGRAGVSFAPGWHPTDFALRPETYPDRRERMFQGIEEVRRLWRGEGRLEPRPVQPELP
                     VWVTCSTNPATWARAGEIGAHVLTIVQPLPTLAERIARYRAARAERGFDPEAGRVTVM
                     VHAFVGDDEEAVREKVRAPMTSYLRTYFDQYGFLAGGAGIPGTKGVRESDVDTLLSIA
                     FEAYFSSLSLLGRPDKGARLVNRLRAIGADEVACLVDFGVDAPSILEALPALARLVAV
                     SGEGRRHQAGQGGH"
BASE COUNT          725 a         1819 c         1837 g          775 t
ORIGIN      
        1 cgatcccggg caggacgatg tcgaccagcg ccagatgggg gaacccgcgc tggccgatga
       61 cgtccagggc ctgctctcca gaggcggcgg ccgagacgtc gaagccggca tcgcagagca
      121 ggcgctcgat gagcgcacgt aggtccggat cgtcctcgac gaccaggatt cggcgcgagc
      181 cgggcccgct ggctctgggc gacgtggaaa actgcacgtt ggtttccctg ggtctcgtct
      241 ccgcccccga gtgtatcccg gttcgtgagc cccgtgccaa gtccccccgc ggagaccagc
      301 cgtaatctcg ttcgtaacgc ttttcccgta tcatcctagc ggggggattg aacgatggcg
      361 agaccgatgg ggagaccggt cgcgagagat tggtgtgtgt catgagcttc gaccctgagt
      421 accaggggtt ccgtacccag ttcaccgacc ggctccctgc ccgcctggag gagatcgagg
      481 ccgcctggcg gcaggtgcgg gagggcggct ggagccgcga gagcctggcg ctcttccacc
      541 gcctggccca ctcgctggcc ggcgcctgcg ccatgttcga cctggcggcc atggcggcga
      601 ccgcccgcga gctggagcac ctgctcaaga ggttggccgt cgagcccggc gaggtctccg
      661 agcggcgcat cgagtcgctc ctggaccgcc tgcggcaggc ggtccccccg aaacatctgg
      721 ccctcctcta gatccatcta gcgcggcacc cggcgaggct gtacaattcg tccaactttc
      781 taggacggat gtagcagttg aatcgccggg actttctcag gacgggtcta ttcgcaggcg
      841 ccggtgtgct cgcggctccg atgctcaatc gggggcgcca cagcctcttc gccgattcct
      901 ccaccgacta ctccacccgc gccatcgacc tggtccggcg ctcggccccg atcgacatgc
      961 tggggctgct gacgctcgac tggaaccgcc tctaccagtg gcagcgccaa ccggcgagct
     1021 tcggcgacgg cgagatccag aagctcacgg gcacaggcac gcgggtcttc cacccggcgg
     1081 tcgacccgaa ccatccggac ccgcacggca cgacgctgcg ttggatctcc ggctggaacc
     1141 gcctcctcgc ggcccacccg gagcggctgg tccgcatcgg ctccgccgac gacctggagc
     1201 gggtccgcga ggggagccgc ctcggcctgc tgatcggctt ccagaactcc gagcatttcc
     1261 gcgactccgc cgacgtggcg ctcttccacg gcctcggcca gcgggtgtcg cagctcacct
     1321 acaacgtccg caaccggcta ggcagcggct gcaaccagga cggccccctc accccgtacg
     1381 gcgccggcat cgtggcggcg atgaacgagg tggggatggc ggtggacatc tcccactgca
     1441 gcgaccgcac cagcttcgac accgtggcgg cgtcgcgcaa gccggtgctc atcacccact
     1501 ccaactgccg cgccctggtg ccgcacaagc gctgcaagcc ggactcggtg atccgcgccg
     1561 tcgccgcccg cggcggcgtc atgggcatca ccgtgatccc ggccttcgcc ggcaagcgcc
     1621 agccggtcac cctggacgac gtgctcgacc acttcgacta cgccgcgcgg ctggtgggcc
     1681 ccgagcacgt cgggctgggg agcgactgcg acaccgacgc catcgacccg aagaccggcc
     1741 gcatccgccc gcgctacgtg gtgcacggcc tgcggcacgt ccggcggacc ttcgacatcg
     1801 cggagggatt gctgcggcgc ggctacgccg agaaggacgt cgagctgatc ctcggcggca
     1861 gcttccggcg cgccctggcg gagatctggg gaccggcgcc agccgcccca acccccgtca
     1921 gagcggccgg ctaggtcccg tcaggcgtac agcccttcga tctcgcgggc gtacttgtcg
     1981 tagatcaccc gccgcttgag cttcatcgtg ggggtcagct cgcccccctc gatgctcagc
     2041 tcctgcggca gcacgtggaa gcgcttgatc gtctcgtagc gcgccaggcc ggcgttgacc
     2101 tgctccacct gcttctccag gtaggcccgg aaggtcggac aggcggcgat ctcctccagc
     2161 gtgcgggcct ggctgccggc ggcggcggct tcggccggcg cgttcgccgg gtcgagggtg
     2221 agcagcgccg ccaggtagtt gcggcggtcc cccaccacca cggcctggga gacggcgcgg
     2281 atctgcttga gcttgctctc caggatctgg gggccgatgt tcttgccgcc cgaggtgatg
     2341 agcagctcct tcttgcggtc ggtgatgcgc agcaggccgt cgccgccgag ctcgccgatg
     2401 tcgccggagt gcagccaccc ctgctcgtcc accgtctccc gggtggccgc ctcgttcttg
     2461 tagtagccca ggaagacgtg cgggccgcgc atcagcacct cgccgtcctc ggcggtcttg
     2521 atctcggtgc cgggcagggg gaagcccacc ttgccggggg tgtagcgctt cggcgtcgag
     2581 aaggtggcgg ggccggtgca ctcgctcatc ccgtagacct ccatgatcgg gatgtcgagg
     2641 ctgaggaaga actgcagggt ctcgacggag atcggcgccg tcgaggtggc gcagatgcgc
     2701 gcccggtcga gccccagctt ctcgcgcacc ttggagaaga cgagcttcct ggccagcgga
     2761 tagaacagcg gcctggaccg cccctcctgc tcggcgcgcc cggtctcggc gccgatgccg
     2821 cgcgcccagg cggcgatctt cttcttgagg ccgctgttct gcgcccccgc cgcctggatg
     2881 gcggcctgca tcttctccca cacccgcggc accgcgaaga agaactgcgg ccggacctcc
     2941 cgcaggttct cccccagctt ctccaggctc tcggcgaacc aggagcagcc gccggtcgcc
     3001 atcgggccgt ggaaggagac cacctgctcg gcgatgtggc tgagcggcag gtagctgagg
     3061 aactgatcgt cggcgttgac ctcgtaggtc tccaccaccc gctgcgccgt ccagacgacg
     3121 ttgtggtggc tgagcgcgac cgccttgggc gggccggtgg tccccgaggt gtagatcagc
     3181 gtgcacaggt cctgtggctt ctgcgcgtcg atgcgggcgc ggaggtccgc ctccggcacc
     3241 tgggccccca tctccaggag ctgcttccag gtgtagatat cgctaccgga cccggaagcg
     3301 tcgccggtca tcagcaccag ggccttgagc ttcgggagct ggccgcggat ggagaggaag
     3361 aggtcgaggt actggcggtt ctcgaccacc acgaccgccg cctcgcagtg gtcggcgatg
     3421 tactggcact gctcggcggc gagggtggtg tagatccccg ccgggtaccc gccggcggcg
     3481 accgcgccga cgtcggcgag gaaccactcc ggccggttga agctcatgat ggccacgccc
     3541 tggccgggct ggaggccgag cttcatgaag ccccgggcgg cgagatgagc ctggtcccgg
     3601 tactccttcc aggtggtggt ccgccaggtg ccaccttcct tgatcttcat cgccgggcgg
     3661 gcgccgtggg tgccggcggt ccgctccagg acgtccatga tcctctcggc catcgctcct
     3721 cctccggttc agagtgacgg cccggtgtag catccgggcc gcgcggctgt caatgcccgc
     3781 cctggcctgc ctggtggcgg cgcccctccc cggacaccgc caccaggcgg gccagggcgg
     3841 gcagggcttc caggatcgac ggcgcgtcga cgccgaaatc gacgaggcag gccacctcgt
     3901 cggcgccgat cgcccgcagg cggttgacca ggcgggcccc cttgtcgggc cggccgagga
     3961 gggagaggga ggagaagtag gcctcgaagg cgatcgacag gagggtgtcc acgtccgact
     4021 cgcgcactcc cttggtaccg ggaatgccgg cgccgccggc caggaagccg tactggtcga
     4081 agtaggtccg caggtacgag gtcatcgggg cgcgcacctt ctcccgcacg gcctcctcgt
     4141 cgtcgccgac gaaggcgtgc accatgacgg tcacccgtcc ggcctccggg tcgaagccgc
     4201 gctccgcccg ggcggcccgg tagcgggcga tcctctccgc cagggtcggc aggggctgga
     4261 cgatggtgag cacgtgggcg ccgatctcgc ccgcccgggc ccaggtggcg gggttggtcg
     4321 agcaggtgac ccagaccggc agctcgggtt ggacgggccg gggctccagc cggccctctc
     4381 ccctccagag ccgccggacc tcctcgatgc cctggaacat ccgttcccgg cggtcggggt
     4441 aggtctccgg ccgcagggcg aagtcggtcg ggtgccagcc gggggcgaag gagacgccgg
     4501 cgcggccgtt ggagagccgg tcgacgaccg cccactcctc cgcgatccgc agcgggtcgt
     4561 ggagcggcag gaccacgctg ccggcgcgaa gaccgagccg ctcggtgacg gcggcgaagg
     4621 cggcgccgag gatcgacgga ttgggataaa agccgccgaa gtgctggaag tggcgctccg
     4681 gggtccagac ggccgcgaag ccgtggcggt cggcgaagcg ggcgctcttt aggaggaggt
     4741 cgtagctctc ggccgggctc ttggcgccgc cgtcggagaa gaagaagagg ctgaagtcga
     4801 ccggccggtc cgagggctcc ggctccggcc cggggtccag ggtgaagagc gggtggccgg
     4861 agggggccgg cgcggccggg accggagcct cgccggggac gcccagcccc tccttccgga
     4921 ggcggcggag cagcagctcg cgctgctccg gggtcagccc ggccaggcgc ttcgagaggt
     4981 catccgccat ggtggaggct ttccagggct tcgagagccg ccatcgcgtc cgcgagggga
     5041 acccggcagg cgacctcgtg ggcgccgatc ttcgcgcagg gctccagctc ggcggacgtg
     5101 aacgcgggga ccgtcaccgt cacgatcccc tgcccgccgg ccgcccgata ggcggc
//
LOCUS       DASPJI010000170         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_411494, whole genome shotgun sequence.
ACCESSION   DASPJI010000170 DASPJI010000000
VERSION     DASPJI010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411494"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VEL74_04525"
     CDS             complement(<1..624)
                     /locus_tag="VEL74_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYG61823.1"
                     /translation="MMLKRWLALCWLVLSVILPSAGWSEAVPTEGFAPVEGGRLFYQV
                     MGEGEPLVLIHGGQLDHRMWDGQFERYAQEYRVIRYDVRGYGKSPASISTPYSASDDL
                     RDLLDHFGVGKAHIAGLSLGGRIASDFAVTYPDRVASLVLIGPGLTGFRDEDEKEGAT
                     FWDFVEAVRDGGPEALADLWLKDPYLAPAATNTALAARLRQLAVENAH"
     gene            complement(631..1071)
                     /locus_tag="VEL74_04530"
     CDS             complement(631..1071)
                     /locus_tag="VEL74_04530"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYG61824.1"
                     /translation="MDQRRRRALLPALLSLALPLRGSADEKPVPVGQVEAEIRALEIA
                     RRDAIQAGDRAALERIYAPDFRGVTSGGLQVGQQQAVGGLLRATGKAAAMDDIEVRVF
                     GSAAVATGRLRLADSGGTLRYIHVYAYREGMWRLVAGQSTDSRE"
     gene            1420..>2202
                     /locus_tag="VEL74_04535"
     CDS             1420..>2202
                     /locus_tag="VEL74_04535"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HYG61825.1"
                     /translation="MSCSQRLRAPGRRARRSLAPVVAVCSLLLPPALSAQSTAPDPAP
                     PDVTVKDEITVTATRSPRPVRETPGAVSVIGEEEIRRLLMTNAADLVKFEPGVYVDSD
                     ATRLGLGGFVIRGMGGNRVLTRIDGVATGERFEFGPLAIPQSTLDVDALKSVEIVRSA
                     GSALYGSDAMGGVVSLMTKEPADFLLGGPSALQGKVGYDGRNRELSESGAVALGADRW
                     QGSLFATRRDGEETRNQGSRDTRDATRTTPNPQDAESLGAIAK"
BASE COUNT          352 a          805 c          720 g          325 t
ORIGIN      
        1 gtgggcgttc tcgactgcga gctgacgcag ccgcgcggcc agggccgtgt tcgtcgcggc
       61 gggagccagg tagggatcct tcagccagag gtcggccagg gcttcggggc cgccgtcccg
      121 caccgcctcc acgaaatccc agaacgtcgc gccctccttc tcgtcctcgt cccggaagcc
      181 ggtgaggccc gggccgatca gcaccagcga ggcaacgcgg tccgggtagg tcaccgcgaa
      241 gtcgctggcg atgcggccgc ccaaagaaag cccggcaatg tgggccttgc ccactccgaa
      301 gtggtcgagc agatcccgca ggtcgtcaga ggcggagtag ggggtggaga tcgacgctgg
      361 cgacttgccg tagccgcgca cgtcgtagcg gatcacccgg tactcctggg cgtagcgctc
      421 gaactgacca tcccacatgc ggtggtcgag ttgaccgccg tggatcagga ccagcggctc
      481 gccctccccc atcacctggt agaacagcct ccccccctcc accggcgcga agccctcggt
      541 gggcacggct tcagaccagc ccgcgctggg caggatgacg gagaggacca gccagcagag
      601 tgccaaccat ctcttcagca tcatcaggcc tcactccctg gagtcggtcg attgtcccgc
      661 cacgagccgc cacatgcctt ctcgataggc atagacgtgg atgtagcgca gagtgccccc
      721 gctgtccgcc agacgtagcc ggccggtggc aacggcggcg ctgccgaaga cccggacctc
      781 gatgtcatcc atcgccgcgg ccttgccggt cgcccgcagc agccctccca ccgcctgctg
      841 ctgcccgacc tggaggcccc ccgaggtcac accgcggaaa tccggggcgt agatccgttc
      901 gagggcggcg cggtctccgg cctggatggc gtccctgcgg gcgatctcca gcgcgcggat
      961 ctccgcctcg acctgcccca ccggcaccgg tttctcgtcg gcgctacccc gcaagggcag
     1021 ggccagggag agcagggccg gaaggagcgc ccggcggcgc cgctgatcca tggagggatt
     1081 ccctgagagc acgttttgca tgacggaatt ctcgggccgc ggcggcatcg ccacaactac
     1141 acggaccggt agtctttacg agattgggca gccagcacta tacacccctg cacgaacgtg
     1201 cagggtgtct tgacaatccg taccgccgcg gtccagaata ggctcgaact attgagacga
     1261 tatctcaata gagagtttgg cgggtcctgc gggtgtaccg tgggcctgaa aagcgaagtg
     1321 gcggctcggt cgagacttgg ggaagaccgc cgaaccgccg cttctggtgc agatccgccg
     1381 cgggcgatag ccctcggctt ctctacggag gcagtataca tgagctgctc ccagcgcctt
     1441 cgggcgcccg ggcggcgtgc gcgccgctct ctcgctcccg tcgtagccgt ctgctccctt
     1501 ctcctccccc ctgccctctc ggcccagtcc acggctcccg atcccgcgcc gcccgatgtc
     1561 acggtgaagg acgagatcac ggtcaccgcg acgcgcagcc cgcgcccggt ccgtgagacc
     1621 cccggcgccg tctcggtcat cggcgaggag gagatccgcc ggctgttgat gaccaacgcc
     1681 gccgacctgg tgaagttcga gcccggcgtc tacgtcgaca gcgacgccac ccgcctcggc
     1741 ctcggcgggt tcgtcatccg cggcatgggc ggcaaccgcg tgctgacccg gatcgacggc
     1801 gtggcgaccg gcgagcgctt cgaattcggc cccctcgcca tcccgcagtc caccctggac
     1861 gtcgacgctc tcaagtcggt ggagatcgtg cgcagcgccg gctccgccct ctacggcagc
     1921 gacgccatgg gcggcgtcgt ctccctgatg accaaggaac ccgcggactt cctgctcggc
     1981 ggcccctccg cgcttcaggg caaagtcggc tacgacggcc gcaaccggga gctgtcggag
     2041 agcggcgccg tcgctctggg agccgaccgc tggcagggat cgctcttcgc cacccggcgg
     2101 gacggcgagg agacccgcaa ccaggggagc cgcgacaccc gggacgccac ccgcaccacc
     2161 cccaacccgc aggacgccga atccctcggc gccatcgcca ag
//
LOCUS       DASPJI010000171         4022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_124258, whole genome shotgun sequence.
ACCESSION   DASPJI010000171 DASPJI010000000
VERSION     DASPJI010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124258"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2234
                     /locus_tag="VEL74_04540"
     CDS             <1..2234
                     /locus_tag="VEL74_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYG61826.1"
                     /translation="LRKLLLVGQRISLVLGVALFAWQLGLIVQAIQKALATEQTLAAL
                     APPKPSWGFYSLATLATIAAIGVLLWRARSSEGAERRRVGMFVGALAFGVAPLFSQIA
                     LGTIFESYGRFIERPDVLPVISGIVFAFFLTLPFTTAYSVLVDHVLGVKLIARRALHY
                     ALARYTALGLATVPLAALAIYLVQNRDQSLAQLFSGSKALLLISAAIVGGAALRYRAM
                     ILDGIDRRFFREQYDARKILTSLVERIRAVNEVAGLASLVSREVDLALHLEGISLLSL
                     DAKGVLVDPRNRARRLDGSSPLALMVSNASDPLEVDLENPRSPLGRLPETERHWLVDN
                     GFKLLVPILARDGSLLGLIALGEKKSGLPFLKEDKQLLHAIASNSAWVLEMARSHSQP
                     PRPPWREEEDAHPALPDPSDMAKECPSCGTLHAPYTVFCSKCSHRLEPSHVPFVLPGK
                     FRFECRIGTGGMGIVYRGFDLALGRAVAVKTLRRVSPEDAMRLRREARTAAAVSHLHL
                     ASVYGMETWQGTPILILELLEGGTLGQRIEAQGRLSPLETVELGIAMAAALAHLHASD
                     ILHRDVKPSNIGYARDGSPKLMDFGIARVMFDLRRDTDTTSIDDLEDNSSILPPTSIW
                     HQSTSSVTFSRQLVGTLSYLSPEALNGRAADVSFDLWGLAIVLFECLLGRKVFSGGDV
                     RQTMSRIRSGRVPDFSQVLPEYDEMLGDFFRAALHKNPARRPATALELKQQLEFLRLR
                     LAA"
     gene            complement(2255..2971)
                     /locus_tag="VEL74_04545"
     CDS             complement(2255..2971)
                     /locus_tag="VEL74_04545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HYG61827.1"
                     /translation="MKHGKGTILHPDQERYLERLLPPRDPVLREMEEQAAREDIPISD
                     PEVGRLLMVLARAAVTAGGTAGGAGGGGRILEVGTAIGYGALCLARGAPEARVVSIDT
                     DAERLATARAYLERAGVAGRVELVQGAALDVLPDLQGPFDLAYVDAVKPEYRRYLDLI
                     LPKLRVGGMVVLDNLLWKGQVAAPPEEGAEDEQATALRAFNTYLMIHPQLTAVVLPYG
                     DGVGLATKTKPLITEMGGPF"
     gene            complement(2968..3933)
                     /locus_tag="VEL74_04550"
     CDS             complement(2968..3933)
                     /locus_tag="VEL74_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61828.1"
                     /translation="MMKKTLPPLALAALVLCASACREEATPVGSLQLQPSQIRLGPGR
                     VQPVRFTWTPTASLEEDAGPPMVFVHLLDGEGKVVRTFDHPFPQRWSEGTPVSYDIEL
                     YQSALAPPLPAGSYRLTAGLYGRGKHRWALEGLGEEIDRAEYVAAQVEVPAQQDGPQF
                     NFSATWLPVEPGGDRQLVARRWLIGRGGIRMVNVRNPGTLWTVVRIPPAEGPDEKLVL
                     KPGQTIPSVLIRGTCGGVEMSVSGPGPHEIEVPIQDVSPEGVCRVVLIPNFHYETPNV
                     RDKRTISLENLAWMPARGGQPATPGAAPRATPASPTAPAPGAPQG"
BASE COUNT          553 a         1500 c         1369 g          600 t
ORIGIN      
        1 gcctgcggaa actcctcttg gtggggcagc ggatctcgct ggtcctcggc gtcgctctct
       61 tcgcctggca actgggcctg atcgttcagg cgatccagaa ggcgctcgcg acagagcaga
      121 cgctagcggc cctggctccc ccaaagccga gttggggctt ctacagcctg gccactctcg
      181 ccaccatcgc cgccatcgga gtcctgctct ggcgggcacg ctcctccgaa ggggcggagc
      241 ggcgccgggt cgggatgttc gtcggcgccc tggcgttcgg ggtcgcgccc ctgttctcgc
      301 agatcgctct agggacgatc ttcgagtcct acgggcgctt catcgaacgg cccgacgtgc
      361 tgccggtgat cagcgggatc gtcttcgcct tcttcctcac cctgccgttc accaccgcct
      421 actcggtgct cgtcgatcac gtgctgggcg tcaagctgat cgcccgccgg gcgctccact
      481 acgccctggc ccgctacacc gcgctcggcc tcgccaccgt gccgctggcc gccctggcca
      541 tctacctggt ccaaaaccgc gatcagtcgc tcgcccagct cttctccggc tccaaggcgc
      601 tgctcctcat ctccgccgcc atcgtcggcg gggcggcgct gcgctaccgg gcgatgatcc
      661 tcgacggcat cgaccggcgg ttcttccggg agcagtacga cgcccgcaag atcctgacct
      721 cgctggtcga gcgcatccgc gccgtcaacg aggtcgccgg cctcgccagc ctcgtctccc
      781 gcgaggtcga cctcgccctc catctcgaag gcatctccct gctctccctc gacgccaagg
      841 gggtgctcgt cgacccgcgc aaccgggcgc ggcgcctcga cggctcatcc cccctggcgc
      901 tgatggtctc gaacgccagc gaccccctgg aggtggacct cgaaaacccc cgctcccccc
      961 tgggtcggct gccggagacg gagcgccact ggctggtgga caacggcttc aagctgctgg
     1021 tgccgatcct cgcccgcgac ggttcgctgc tcggcctgat cgcgctgggc gagaagaaga
     1081 gcggcctccc cttcctcaag gaggacaagc agctcctcca cgccatcgcc agcaactccg
     1141 cctgggtgct ggagatggcg cggagccact cgcagccgcc gcggcccccc tggcgcgagg
     1201 aggaggacgc ccatccggca ctcccggacc ccagcgacat ggccaaggag tgcccgtcct
     1261 gcggcacgct gcacgcgccc tacacggtgt tctgcagcaa atgcagccac cggctggagc
     1321 cctcccatgt ccccttcgtg ctgccgggca aattccgttt cgagtgccgc atcggcaccg
     1381 gcggcatggg gatcgtctac cgcgggttcg acctggcgct gggccgggcc gtggcggtca
     1441 agacgctgcg ccgtgtctcc cccgaggacg ccatgcggct gcggcgcgag gcacggacgg
     1501 cggcggccgt ctcccacctc cacctcgcct cggtctacgg catggagacc tggcagggga
     1561 cgccgatcct gatcctggag ctgctcgaag gggggaccct ggggcagcgc atcgaggcgc
     1621 aggggcggct ctcccccctg gagacggtgg agctgggcat cgccatggcc gcggccctcg
     1681 cccacctgca cgcctccgac atcctccacc gcgacgtcaa gccgagcaac atcggctatg
     1741 cccgggacgg cagccccaag ctgatggact tcggcatcgc gcgggtcatg ttcgacctac
     1801 gccgggacac cgacaccacc tcgatcgacg acctcgagga caactcctcg atcctgccgc
     1861 cgacctccat ctggcaccag agcacctcct cggtgacctt ctcgcgccag ctcgtcggca
     1921 ccctctccta cctctccccg gaggcgctga acggccgggc ggcggacgtc tccttcgacc
     1981 tctgggggct cgccatcgta ctcttcgaat gcctgctggg aagaaaggtc ttctccggcg
     2041 gcgacgtgcg gcagaccatg agccgcatcc gctccgggcg ggtgccggac ttctcccagg
     2101 tcctccccga gtacgacgag atgctggggg atttcttccg cgccgccctg cacaagaacc
     2161 cggcgcgccg ccccgccacc gcgctggagc tcaagcagca gctagaattc ctgcggctcc
     2221 gtctggccgc ctaggtggcc gcccaggctg ccgcctagaa cggcccgccc atctcggtga
     2281 tcagcggctt ggtcttggtg gcgagaccga cgccgtcgcc gtaggggagc accaccgccg
     2341 tgagctgggg gtggatcatc agataggtgt tgaaggcgcg tagcgccgtg gcctgctcgt
     2401 cctcggcgcc ttcctcgggg ggcgccgcca cctgcccctt ccagagcagg ttgtccagga
     2461 ccaccatccc tcccacccgc agcttcggca ggatcaggtc caggtaccgc cggtactccg
     2521 gcttgaccgc gtccacatag gccaggtcga aggggccctg gaggtccggc agcacgtcca
     2581 gggccgcccc ctggaccagc tcgacccggc cggccacgcc ggcccgctcc aggtaggcgc
     2641 gggcggtggc gaggcgctcg gcgtcggtgt cgatggagac cacccgggcc tccggcgcgc
     2701 cgcgggccag gcagagggcg ccgtagccga tggcggtgcc gacctcgagg atccgcccgc
     2761 cgccgccggc accgccggca gtacctccag cggtaacggc cgcccgggcg aggaccatca
     2821 gcaggcgccc gacctcgggg tcggagatgg ggatgtcctc ccgcgccgcc tgctcctcca
     2881 tctcgcggag gaccgggtcg cgcggcggga ggagccgctc gaggtagcgc tcctggtccg
     2941 ggtggaggat cgtccccttt ccgtgcttca tccctggggt gcgcccggcg cgggggccgt
     3001 gggggacgcc ggagtggccc gcggagcagc ccccggggtg gccggctggc cgccccgcgc
     3061 cggcatccag gcgaggttct cgagcgagat cgtccgcttg tcgcgcacgt tcggcgtctc
     3121 gtagtggaag ttggggatca gcacgacccg gcagacgccc tccggcgaca cgtcctggat
     3181 cggcacctcg atctcgtgcg gccccggccc ggagacgctc atctcgacgc cgccgcaggt
     3241 gccgcggatc agcaccgacg ggatggtctg ccccggcttg agcaccagct tctcgtccgg
     3301 cccctcggcg ggggggatgc gcaccaccgt ccacagcgtt ccggggttgc gcacgttcac
     3361 catccggatg ccgccgcggc cgatgagcca gcggcgggcc acgagctggc ggtcgccgcc
     3421 gggctcgacg gggagccagg tggcggagaa gttgaactgc ggcccgtcct gctgcgccgg
     3481 cacctcgacc tgcgccgcca cgtactcggc gcggtcgatc tcctcgccca gcccttcgag
     3541 cgcccagcgg tgcttgcccc ggccatagag gccggcggtc agccggtagg agccggcggg
     3601 gagcggcggc gccagcgccg actgatagag ctcgatgtcg tagctcaccg gcgtcccctc
     3661 gctccagcgc tgcgggaagg ggtggtcgaa ggtacgcacc accttgcctt cgccgtccag
     3721 caggtggacg aagaccatgg gggggccggc atcctcctcc agggaggcgg tgggggtcca
     3781 ggtgaagcgc accggctgca cccggcccgg gccgagccgg atctgggagg gctggagctg
     3841 aaggctcccg acgggcgtgg cctcctcgcg gcaggcggag gcgcacagga cgagagcggc
     3901 gagcgcgagc ggcggcagag tctttttcat catgagagag gacacgctag cgagggtgga
     3961 gcgagctgtc aagccggaca cggccccacg gaccgacacg gaccctcacg gaccgcggga
     4021 gc
//
LOCUS       DASPJI010000172         4009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_241595, whole genome shotgun sequence.
ACCESSION   DASPJI010000172 DASPJI010000000
VERSION     DASPJI010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241595"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="VEL74_04555"
     CDS             <1..1252
                     /locus_tag="VEL74_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HYG61829.1"
                     /translation="PATALRYPADQGSLSRAVLRCVGVGRCRREEGGTMCPSYRVTGE
                     EKHSTRGRARLLYEMLAGEVVTDGFRSEEVREALDLCLACKGCKSDCPAGVDMASYKA
                     EFLHHYYKGRPRPVQAYAVGLVAWWARAGSLAPGLANALLRTRIAKRLGGIAPERRLP
                     SLARETFRAAFGRRNTALEGGEGEPVLLWPDTFTDFFHPEIGMAAADVLEAAGYRVTL
                     PRRRLCCGRPLYDSGMLGLARRWLGRILETLAPEIDAGVPLVVLEPSCAAVFRDELLQ
                     LFPEDPRARRLSALSRTLAELLADSPFAPSRLEERAVVHGHCHHKAVMGMAADRTLLA
                     RLGLDFEILDSGCCGMAGGFGFEKDHYEVSIACGERVLLPAVRATTPDTLVIADGFSC
                     REQIAQATGRRALHLAQVARMAVG"
     gene            1293..2366
                     /locus_tag="VEL74_04560"
     CDS             1293..2366
                     /locus_tag="VEL74_04560"
                     /inference="COORDINATES: protein motif:HMM:NF016629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HYG61830.1"
                     /translation="MSDFFSGIRLSPFGRSSPWNGGEMGAHDGVYKLLFAHARVVMDL
                     LRGFVREPWVRQLDFATLERVSGEFMSVDLRNRHNDVVWRLLWRRPDSVPTPVYLLLE
                     FQSRPDRFMAVRLLTYLALLYEDIIRHRALTPSGKLPPVLVMVVYNGNRRWNAAREIE
                     SLIEPAPGMLGRSCPRFTYAVLDERRLAFGELGDASNLAVALIRLEVSSSREEILTVA
                     AEVAAQLPRGQEPELRQAFTSWLLRALRRAHPGAAIPEISELEEIPMLEENLSRWVRE
                     DRRKARKEGREEGRKRGLKEGRKEGQVEGARKLLLRQLERRFGPLAPEVRQRVEAISS
                     ARQLEAMADEILVAASLRDLGLE"
     gene            complement(2397..3275)
                     /locus_tag="VEL74_04565"
     CDS             complement(2397..3275)
                     /locus_tag="VEL74_04565"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HYG61831.1"
                     /translation="MSSGTAGIAEMEQSAEAPEEARGVVDTDAMQAKSAPAASGAEPA
                     PAANQASKPAVTRKLVRTVDLNLEVKSTEEAAAAAQALANRVGGYVGNVEAQRREGVL
                     YYQITLRVPVEQLEATLSEIKKMAVSVNHESQTVHDVTDQYVDLDARIRTLQATEREL
                     QALLAESRQRGSKVEGIMAVYRELTGIRSQVEQIQAQLKSLEQLAALSTISLTLNPVE
                     GAKPVAPDVWNPGGTLRMSLRTLVILLRAAGDFAIFAVVVLLPIGLAVWGVVWLLKKL
                     RGARGPVAPPAVGGGQ"
     gene            3425..>4009
                     /locus_tag="VEL74_04570"
     CDS             3425..>4009
                     /locus_tag="VEL74_04570"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HYG61832.1"
                     /translation="MTEKTTHRIRAHRGDARERLDLALVRHLEEIPGLSRTRIQEWIA
                     DGMVRVNGAPARRASERLAAGDEVEIDLPPPPPRPEMAAQEIPLSILYKDEHLVAVDK
                     PPGLVVHPTYGHHDGTLLNALLWHLRQDGKEPGTLGPVSRLDKWTSGVLVVARGGEAH
                     AKLARALRSTISEKEYLAVVYGRTGVDRGRIDLWV"
BASE COUNT          580 a         1376 c         1397 g          656 t
ORIGIN      
        1 gcccgccacc gccctgcgct acccggcaga ccaggggagc ctctcccgcg ccgtgctgcg
       61 ctgcgtgggg gtcggccgct gccggcgcga ggaggggggg accatgtgcc cgagctaccg
      121 ggtgaccggc gaggagaagc actcgacgcg ggggcgcgcc cggctcctgt acgagatgct
      181 ggcgggcgag gtggtgacgg acggcttccg gagcgaggag gtgcgcgagg cgctggacct
      241 ctgtctcgcc tgcaaggggt gcaagagcga ctgcccggcc ggggtggaca tggcttccta
      301 caaggccgag tttctccacc attactacaa gggccgcccg cggccggtcc aggcctatgc
      361 cgtgggcctg gtcgcctggt gggcgcgggc aggctccctg gcgccggggc tcgccaacgc
      421 cttactccgg acgcggatcg cgaagaggct tggaggcatc gccccggagc gccgccttcc
      481 ctccttggcc cgcgagacct tccgggccgc cttcggccgc cggaacacgg cattggaagg
      541 gggggaaggc gagcccgtcc tcctctggcc cgacaccttc accgacttct tccatcctga
      601 gatcgggatg gccgcggccg acgtgctgga ggccgcgggc taccgggtca ccctcccccg
      661 ccgccgtctc tgctgcggcc ggccgctcta cgactccggc atgctgggcc tggcacgccg
      721 ctggctgggc cggatcctgg agaccctggc acccgagatc gacgccggcg tgcccctggt
      781 ggtcctggag ccgagctgcg ccgcggtctt ccgggacgag ttgctccagc tctttcccga
      841 ggatccccgg gcgcggcgcc tgtccgccct ctcccggacc ctggccgagc ttctcgcgga
      901 ctcacccttc gcgccgtcga ggctcgagga gagggcggtc gtccacggcc attgccatca
      961 caaggcggtc atggggatgg cggccgaccg gaccctgctc gcccggctcg gcctggattt
     1021 cgagatcctc gactccggct gctgcggcat ggccggcggc ttcggcttcg agaaggacca
     1081 ctacgaagtg tcgatcgcct gcggcgaacg ggtgctcctc cccgccgtcc gcgccacgac
     1141 cccggacacc ctggtgatcg ccgacggctt cagttgccgc gagcagatcg cccaggccac
     1201 cggtcggcga gcgcttcacc tggcccaggt ggcgcgcatg gcggtgggat gaggtggtcc
     1261 ggccccgatc aggtatttag acacgcttaa aattgagcga ttttttctcc ggaatacgtc
     1321 tttccccgtt cggacgcagt tcgccttgga acggaggaga gatgggagca cacgatggcg
     1381 tgtacaagct gctttttgcc catgcgcgcg tggtcatgga cctgctgcgc gggttcgtgc
     1441 gggagccctg ggtgaggcag ctcgacttcg cgactttgga gcgggtcagc ggtgagttca
     1501 tgagcgtcga cctgcggaac cgccacaacg acgtggtctg gcgcctgctc tggcggcgac
     1561 ctgattccgt gccgactccg gtgtacctgc tgctcgagtt ccagtcgcgg cctgaccgct
     1621 tcatggcggt gcgcctgttg acctacctgg cccttctcta tgaggacatc atccgtcatc
     1681 gtgccctcac gccgtcgggc aagttgccgc cggtgctggt catggttgtc tataatggga
     1741 accggcgctg gaacgcggca cgggagatcg agagtctgat cgagccggct ccgggaatgc
     1801 tgggacgttc gtgcccccgc tttacctatg cagtcctgga cgagaggcgg ctcgcttttg
     1861 gagagctggg agatgcctcg aacctggccg tggcgctgat ccgcttggag gtcagcagct
     1921 ccagggagga gatcctgaca gtggcggccg aggtcgcggc gcagctaccc cgcggccagg
     1981 agccggagct acggcaggcg ttcaccagtt ggttactgcg cgcactgcgc cgggcgcatc
     2041 ccggagccgc gattccggag atttctgagc tggaggagat tccgatgctc gaagagaacc
     2101 tgagccgttg ggtgcgtgag gatcgacgga aggccagaaa agaaggccga gaagagggtc
     2161 gcaagagagg tctcaaagaa ggccgaaaag aaggccaggt cgagggtgcc cggaagctcc
     2221 tgctgcgcca gctggagcgg cgcttcggac ctttggctcc ggaggtccgc cagcgggtcg
     2281 aggccatctc gtcggcccgc cagctcgagg cgatggccga cgagatcctg gtggcagcct
     2341 ccctgcgcga cctgggtctc gagtagaccg cacacgagcc caggtcgccg tggggattac
     2401 tgtcctcctc cgacggccgg cggcgcgacc ggcccgcggg ccccccgcag cttcttcaac
     2461 agccagacca ccccccacac cgcgagtccg atcggcagca gcaccacgac cgcgaagatc
     2521 gcgaagtcgc cggcggcgcg caggaggatg accagagtcc gcaggctcat ccgcagcgtc
     2581 cccccggggt tccagacgtc gggggccacc ggcttcgcgc cctccaccgg gttcagggtg
     2641 aggctgatgg tggagagcgc ggcgagctgt tccaggctct tgagctgcgc ctggatctgc
     2701 tcgacctggg agcggatgcc ggtcagctcc cggtagaccg ccatgatgcc ctcgaccttg
     2761 ctcccccgct gccgggactc ggcgaggagc gcctggagct cccgctccgt ggcctgcagg
     2821 gtgcggatgc gggcgtcgag gtccacgtac tggtcggtga cgtcatgcac cgtctggctc
     2881 tcgtggttga cgctgacagc catcttcttg atctcggaca gggtggcttc gagttgctcc
     2941 accggcacac gcaaggtgat ctggtagtag aggaccccct cccgccgctg ggcttcgaca
     3001 ttgcccacgt agccgccgac ccggttggcc agcgcctggg ccgccgccgc ggcttcctcc
     3061 gtggacttca cctccaggtt gaggtccacg gtgcggacca gcttgcgcgt caccgcgggc
     3121 ttggaggcct ggttggccgc cggtgccggc tcggcgcccg aggctgccgg cgcggacttc
     3181 gcctgcatgg cgtccgtgtc gacgacgccc cgggcctcct ccggcgcctc cgcgctctgc
     3241 tccatctcgg cgatccccgc cgtaccggac gacatctcgc cttcttcgag tctgctgcac
     3301 gcggacagac ccagtccggc cagcaggagg aggaccatcc atccatgtct cgtcacatgt
     3361 cacctcgttc gggagtcggc tctatcatga cacgcccccg gctatggtga gatagacacc
     3421 cgagatgacc gagaagacaa cccatcgtat ccgcgcccac cgcggggacg cccgggagcg
     3481 gctggacctg gcgctcgtcc ggcacctgga ggagatcccg gggctgtcgc gcacccggat
     3541 ccaggaatgg atcgcggacg ggatggtgcg ggtcaacggg gcgcctgcgc gccgggcgtc
     3601 ggagcgcctc gccgccggcg acgaggtgga gatcgacctg cctcccccgc cgccgcggcc
     3661 ggagatggcg gcgcaagaga tccccctctc catcctttat aaggatgagc acctagtcgc
     3721 cgtggacaag ccgcccgggc tggtcgtcca tcccacctac ggccaccacg acggcacgct
     3781 cctgaacgcc ctgctctggc atctgcggca ggatgggaaa gagcccggaa ccctggggcc
     3841 ggtcagccgt ctggacaagt ggacctccgg cgtcctcgtc gtcgcccggg gtggcgaggc
     3901 gcacgccaag ctcgcccggg ccctccggtc caccatctcc gagaaggagt acctggcggt
     3961 ggtctacggc cggaccggcg tggaccgcgg gcgcatcgac ctgtgggtg
//
LOCUS       DASPJI010000173         9495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_124342, whole genome shotgun sequence.
ACCESSION   DASPJI010000173 DASPJI010000000
VERSION     DASPJI010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9495)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9495
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124342"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VEL74_04575"
     CDS             <1..596
                     /locus_tag="VEL74_04575"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HYG61833.1"
                     /translation="LMLKRALDVALASLLLLLALPAVGLVAALIKLTSGGTVLFRQTR
                     CGLNGRFFTLYKFRTMVEDAEERRNALLHLNEMNGPVFKLRSDPRVTWFGRFLRRFSL
                     DELPQLWNVLRGDMSLVGPRPPIPEEVARYQRWQRRRLAMKPGLTCLWQISGRNNVDF
                     DRWMQLDLEYIDSWSPMLDFKILLKTIPAVLSGRGAS"
     gene            856..3045
                     /locus_tag="VEL74_04580"
     CDS             856..3045
                     /locus_tag="VEL74_04580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG61834.1"
                     /translation="MNVSVVVIAYNEERKIAACLGALAAQDYRAGEVEIVLVDGGSTD
                     RTAEVARAAVPSVKVVPNPGGSISGNRNRGWRAASHPLVAFVDADCVAPSHWLRTLLA
                     GMAEAEGRVAAVGGANVPPAGESPFYDALAVMLDTYAGSRGSVQGRVYEDTREVDHLP
                     GLNVLYRRAALEAVAGWDERFGFIGEDEDLSHRLRGQGFRLLYVPGAAVVHRQRGDFR
                     SWSRNMFLYGKGRVWLIRRHPRAFRLSFLVPPLLPLLLWAYLPAVLLLAAWYGLRAGR
                     PGVIPRLALLFATTHIAYGLGEWAGVFTPGDSPKARESARRGRRRKVGLIVLKNAGNK
                     GDEAIFVSVCRRWLLRDREEGAPLPYPLYALGFGPSGFDVRLVPEDLAGVERLAADLC
                     RADPGARRLGGQALGQGVTLLWVLVTFRAVLFCGGQWIHDLNPAFHAAISGLLAFGRL
                     TGTRMGVFCVGSGPLRAGWSRLAVRLAFGRRALLVVRDPVSADLYRKAGLPQVRLAVD
                     PALELPTSQPPDLPEGSTVGLSPCAWFRFENVYRRDPAVIAEMVTVLVRLVDGLRRRG
                     LRVLLVPTMNPEDREICRSLLDALPEDRFLRPLPPVSLLDTGALTPSQIQGAIAGLGV
                     LISMRLHPVIFAFNVGTPFVALNYAEKVAEFCRRAGLEDRLVQLDGDWGGESLRRLDA
                     SDSEALRRRMAGEHARLAAELEPAYQALWAWLARPGAAPRPVSSSAC"
     gene            3095..4744
                     /locus_tag="VEL74_04585"
     CDS             3095..4744
                     /locus_tag="VEL74_04585"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HYG61835.1"
                     /translation="MKIFFLNPPYRDQRFSRSQRSPGVIKSGTMYYPYWLAHAAALCE
                     SRGHEIFLLDAPADNRGRQDVIDSIRDFGPGLIVSETSTPSFHYDLETLGVLRDAFPA
                     AHICLVGTHATAEWQKALERCPALDTVGIGEYDFIILDLADALQTPGADLRKIPALGF
                     RDQDGKPGRGPARLPIEDMDSLPWIAPVYKRFLTPENYLFTIASHPMVMLIGGRGCTA
                     RCFFCVYPQVMHGHVYRTRSPEHLVGEMKWIQDNMPEVKEIVFEDDTFTSDKPRAREI
                     ARLVKEQGVRLPWFANIRTNVDHDTLAALKESGLRECATGFESGDDLVLINMRKGQNV
                     SMQRKFMESARELGILVHGCFMVGFPGETRETMQKTLDLAIELDPDSAQFYPVMPYPG
                     TGAFQWAKDHGYLATHDYNQWLTDDGGHRCVMNLPGLTPTQIEEFCETAFRKFHFRPA
                     YIARKLKQMVTDPKEGKRSINAGLNFVRYLFTNEREKERPFDAPEIPVTDEWNAKIKT
                     PYGRMERVETVVRKGFDGRPIDDPALRDAAVLQVEQESQRL"
     gene            4758..5681
                     /locus_tag="VEL74_04590"
     CDS             4758..5681
                     /locus_tag="VEL74_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYG61836.1"
                     /translation="MAFPLNADTFRCPACRGTLRREADRLACAGCGASYEVRDEIPVF
                     FEAGDEADLQEMAELVARLEAAPASDLAGVAPHFRLPTRRHDLGRRTAEEKSFRGFFR
                     RFPDLSRLRILDVSCGVGREAQILLERGASEVHLLDISYPAVRYARHAFGRFYPDRRL
                     AFSVSDAGRLPYADGTFDLVLVYGSGHHYPDLDGFLSEARRVAGRVVLLAEPARMGAA
                     QRLLDAMGWNTEYGGLDTHRFDEAELRRRLAAHGLRCETERLFQYFPKALARMGDVRP
                     FVAAWFGLLGVLDVCVPRDLRHSLNVYADRG"
     gene            5665..6726
                     /locus_tag="VEL74_04595"
     CDS             5665..6726
                     /locus_tag="VEL74_04595"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HYG61837.1"
                     /translation="MPTGAEAPRAGVPGTAGEYDAVQSRRRLLGWLLYGFGLSLLVAL
                     IVRTGPWRMLEALLESRWQPVALAILALAIGQTLRGIKWAYLIGQVQPGVDRPLVVGC
                     YFTNILVSYWTPARSGEALAPLLLASRCGTPKRLGLAVILVDRMADVAFLAAALVGSA
                     LYLGSHAGSAEGSGTLRTSLYAAAAFAAVLGAAGVAVLRATRPGLAARQALEGDLPPE
                     DGLFGRLTGLFWKLRAAIAQVVRPRRLGVLWILTMAAWLFDFANLYWMVNAVTPIRFV
                     ESSVAQSVAAGASLASFVPGGIGVATASYVVVADLLGADWQRVAAAGVLAVVLNQVTR
                     FALALAAGAHWGRAAKRLR"
     gene            6830..7123
                     /locus_tag="VEL74_04600"
     CDS             6830..7123
                     /locus_tag="VEL74_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61838.1"
                     /translation="MSPKICRNVVALAALAAALSVSPLQAAGPQLREARPETPWQAVA
                     SFWSAATHQVVSWWKHSQQPSPQKPSCQNGAISCSNPKPPGEVGSAIDPDGKP"
     gene            7294..8607
                     /locus_tag="VEL74_04605"
     CDS             7294..8607
                     /locus_tag="VEL74_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG61839.1"
                     /translation="MEDHPTPESLQELVRGGLPAERTRAVVAHLVQGCGRCLAVVVSR
                     YQALVGLPRVEHPLTPEQDAGYDAAIDRAFAAVRAFVARARPAAAGGEPAAMASPDSL
                     RLDTLLAQSSAARYQSPERMIELAEEATRVAASLTSPGPGLHTPRQIADLQCRAWAEL
                     GNAYRVADDLGGAEDALGRAAELFTRGTGDESLRARLFDLLASFYGARRRFEMARTAL
                     EVALGIYRRQGDGQLAGRALLKLGTYTGYAGRPEEAIRLTREGLARLDEERDPGLVFV
                     AVHNLARWLTDCGQFREARKLLWQNRARHEHAGGDLNLLKIRWLEGEINAGLGELERA
                     ENAFRAVHEGFAEAGLRYTAAVAALDLATVWLRQGRDAEARHLALEAAQVFLVLRIHR
                     EALGAVLILQKALEVSQASAALLESVARFLRRAEDDPTARFEPKA"
     gene            complement(8608..>9495)
                     /locus_tag="VEL74_04610"
     CDS             complement(8608..>9495)
                     /locus_tag="VEL74_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYG61840.1"
                     /translation="GSDEYARASLPLLHDAGLPAAELDLAEAARRFPQVRFDGIRSVF
                     VEKEAGFLAARRGCRVVAEALAAEGGEYRQLAVRPGALEGGRMGRLELSDGSHLEADA
                     YVFACGPWLGRVFPDAVGGKIRPTRQEVFYFGTPAGDPSYSEGRFPVWFEFGELVHYG
                     IPGNDHRGFKWADDTRREETDPTSMDRTPTPERLEAARRHLSHRFPGLAGAPVVESRV
                     CQYENSPDGHLFIDRHPEARNAWIVGGGSGHGYKFGPALGEHVAGVVLGETEPLARFA
                     LGREAMPPPVTLPAGVTAL"
BASE COUNT         1214 a         3589 c         3313 g         1379 t
ORIGIN      
        1 agctgatgct caagcgggcg ctcgacgtgg cgctcgcctc gctgctgctg ctgctcgccc
       61 tgccggcggt ggggctggtg gcggcgctga tcaagctgac ctcgggcggc accgtgctct
      121 tccggcagac ccgctgcggc ctgaacggcc gcttcttcac cctctacaag ttccgcacga
      181 tggtggagga cgccgaagag cggcgcaacg ccctgctgca cctcaacgag atgaacggcc
      241 cggtgttcaa gctgcgcagc gacccgcggg tgacctggtt cggccgcttc ctgcgccgct
      301 tcagcctcga cgagctgccg cagctctgga acgtgctgcg cggcgacatg agcctggtgg
      361 ggccgcgccc gcccatcccg gaggaggtgg cgcgctatca gcgctggcag cgccggcgcc
      421 tcgccatgaa gccgggcctc acctgcctct ggcagatcag cggccggaac aacgtcgact
      481 tcgaccgctg gatgcagctc gacctcgagt acatcgactc ctggtcgccg atgctcgact
      541 tcaagatcct gctcaagacc atccccgccg tgctgagcgg ccgcggcgct tcgtaggcct
      601 cccgcaggcg agaacgcgcg tccggacgcc gtggatgccg tccaggcgct ccggacgctt
      661 cccggacgcc gcggacgcct cccgattcat ccggatggat tccggacgcc ccgggaagca
      721 tccggacgcc tcggatcgcg tcccgttcaa cgggatggat tccgcaaggc ccgggacgct
      781 tccccgacgc tccggacggc gtccgggccg tctggacggt gtccgggcgc tccggtcggg
      841 taaactcagg gccccgtgaa cgtcagcgtc gtcgtcatcg cctacaacga ggagaggaag
      901 atcgccgcct gcctcggcgc cctcgccgcc caggattacc gggccggcga ggtggagatc
      961 gtgctggtgg acggcggctc gaccgaccgc accgccgagg tggcgcgtgc cgcggtgccg
     1021 tcggtgaagg tggtccccaa ccccggcggc tcgatcagcg gcaaccgcaa ccgcgggtgg
     1081 cgagcggcgt cccatccgct cgtcgcgttc gtggacgccg actgcgtggc gccctctcac
     1141 tggctccgga ctctgctcgc ggggatggcg gaggcggagg gcagggtggc ggcggtgggc
     1201 ggggccaacg tgccgccggc cggcgagtcg ccgttctatg acgcgctggc ggtgatgctc
     1261 gacacctacg ccggctcgcg cggctcggtg caggggcggg tctacgagga tacccgggag
     1321 gtggatcacc tgccggggct gaacgtcctc taccggcgcg cggccctgga ggcggtcgcc
     1381 ggctgggacg agcgcttcgg cttcatcggc gaggacgagg acctctccca ccggctgcgc
     1441 ggccaggggt tccggctcct ctatgtgccg ggggccgcgg tggtccatcg ccagcgcggg
     1501 gatttccgga gctggtcccg caacatgttc ctctatggga agggacgggt ctggctgatc
     1561 cggcgccacc cgcgggcgtt ccggctgtcg ttcctggtgc cgccgctgct gccgctgctc
     1621 ctctgggcct acctgccggc ggtgctgctc ctcgcggcct ggtacggcct gcgggcgggg
     1681 aggcccggcg tcatcccgcg cctggccctg ctcttcgcca ccacccacat cgcctacggg
     1741 ctgggggagt gggccggggt cttcaccccg ggcgactcac ccaaggcgcg ggagagcgcc
     1801 cgccgggggc gccggaggaa ggtggggttg atcgtcctca agaacgccgg caacaagggg
     1861 gacgaggcga tcttcgtcag cgtctgccgg cgctggctgc tgcgcgatcg ggaggagggg
     1921 gcgccgctgc cgtaccccct ctacgcgctg ggcttcgggc ccagcggctt cgacgtgcgc
     1981 ctggtgccgg aggacctcgc cggcgtcgag cggctggcgg cggacctctg ccgggccgat
     2041 cccggggcgc gccgtctggg gggacaggcg ctgggacagg gggtgacgct cctctgggtg
     2101 ctcgtcacct tccgcgccgt cctcttctgc ggcgggcagt ggatccacga cctgaacccg
     2161 gccttccacg ccgccatctc ggggctgctg gccttcggcc ggcttaccgg gacgcgcatg
     2221 ggggtcttct gtgtcggctc ggggccgctc cgcgccgggt ggtcccgcct cgccgtccgg
     2281 ctcgccttcg gccggcgggc cctgctggtg gtgcgggacc cggtctcggc cgacctctac
     2341 aggaaggcag ggctccccca ggtccgcctg gccgtggacc cggcgctgga gctgccgacc
     2401 agccagccgc ctgatctgcc ggaggggagc accgtcggcc tctccccctg cgcctggttc
     2461 cgcttcgaga acgtctaccg gcgcgacccg gcggtcatcg cggagatggt gacggtgctg
     2521 gtgcgcctgg tggacggcct ccgccgccgg gggctccgcg tcctcctggt ccccaccatg
     2581 aacccggagg accgggagat ctgccggagc ctgctggacg ccctgccgga ggaccgcttc
     2641 ctgcgaccac tgccgcccgt ctccctgctg gacaccggcg ccctcacgcc ctcgcagatc
     2701 cagggcgcca tcgccgggct cggcgtgctg atcagcatgc gcctccaccc ggtgatcttc
     2761 gccttcaacg tggggacccc cttcgtcgcg ctcaactacg ccgagaaggt ggccgaattc
     2821 tgccgccggg ccgggctgga ggaccgcctc gtccagctcg acggcgactg ggggggcgag
     2881 tcgctccgcc ggctggacgc ctccgactcg gaggcgctcc gccgccgcat ggccggggag
     2941 cacgcccggc tggcggccga gctggagccc gcctaccagg cgctctgggc gtggctcgcc
     3001 cgcccgggag ccgccccccg gcccgtctcg tcgagcgcgt gctaagattc cccgcccagc
     3061 gtacgctcgc gtccgcccgg aggccgtcta tcccatgaag atcttcttcc tcaatccccc
     3121 gtaccgggat cagcgcttct cgcgctccca gcggagcccg ggggtgatca agtcgggcac
     3181 catgtactac ccgtactggc tcgcccatgc cgccgccctc tgcgagtcca gggggcacga
     3241 gatcttcctg ctcgacgccc cggccgacaa ccgggggcgc caggacgtca tcgacagcat
     3301 ccgcgacttc ggcccggggc tcatcgtctc cgagaccagc accccgagct tccactacga
     3361 cctcgagacc ctcggggtcc tgcgcgacgc cttccccgcg gcccacatct gcctggtggg
     3421 gacccatgcc accgccgagt ggcagaaggc gctcgagcgg tgcccggccc tcgacacggt
     3481 ggggatcggc gagtacgact tcatcatcct cgacttggcc gacgccctgc agacgccggg
     3541 cgcggacctg cgcaagatcc cggctctcgg cttccgcgac caggacggca agcccgggcg
     3601 cggcccggcg cggctgccga tcgaggacat ggactcgctc ccctggatcg cgccggtcta
     3661 caagcgcttc ctcaccccgg agaactacct gttcaccatc gccagccatc cgatggtgat
     3721 gctgatcggc ggccgggggt gcacggcgcg ctgcttcttc tgcgtctacc cgcaggtgat
     3781 gcacgggcac gtctaccgca cccgctcgcc cgagcacctg gtgggcgaga tgaagtggat
     3841 ccaggacaac atgccggagg tgaaggagat cgtcttcgag gacgacacct tcacctccga
     3901 caagccgcgg gcgcgcgaga tcgcccgcct ggtcaaggag cagggggtgc ggctcccctg
     3961 gttcgccaac atccgcacca acgtcgacca cgacaccctg gcggctctca aggagagcgg
     4021 cctccgggag tgcgccaccg gcttcgagtc gggggacgac ctggtgctga tcaacatgcg
     4081 caagggccag aacgtctcca tgcaacgcaa gttcatggag agcgcccgcg agctcggcat
     4141 cctggtccac ggctgcttca tggtcggctt ccccggcgag acccgggaga cgatgcagaa
     4201 gaccctcgac ctcgccatcg agctcgaccc ggacagcgcc cagttctacc cggtgatgcc
     4261 ctacccgggc accggcgcct tccagtgggc caaggaccac ggctatctcg ccacccacga
     4321 ctacaaccag tggctcaccg acgacggcgg ccaccgttgc gtcatgaacc tgccgggtct
     4381 caccccgacg cagatcgagg agttctgcga gaccgccttc cgcaagttcc acttccggcc
     4441 ggcctacatc gcccgcaagc tcaagcagat ggtcaccgac cccaaggagg ggaagcgctc
     4501 catcaacgcc gggctgaact tcgtgcgcta cctgttcacc aacgagcgcg agaaggagcg
     4561 gccgttcgac gcgccggaga tcccggtgac cgacgagtgg aacgccaaga tcaagacccc
     4621 ctacggccgc atggagcggg tcgagaccgt cgtccgcaag ggcttcgacg gccgcccgat
     4681 cgacgatccg gcgctgcggg acgcggcggt cctccaggtc gagcaggaat cccagcggct
     4741 gtgagcggct tcgagcgatg gccttcccgc tgaacgccga caccttccgc tgcccggcct
     4801 gccgcggcac cctgaggcgt gaggcggacc gcctggcctg cgccggctgt ggcgcctcct
     4861 acgaggtgcg cgacgagatc ccggtcttct tcgaggccgg ggacgaggcg gacctgcagg
     4921 agatggcgga gctggtggcg cggctcgaag cggcccccgc ttccgacctc gccggcgtcg
     4981 ccccccactt ccggctcccc acccggcgcc acgacctggg gcggcggacc gccgaggaga
     5041 agtcgttccg cggcttcttc aggcgtttcc ccgacctctc gcgcctgcgc atcctggacg
     5101 tctcctgcgg cgtcggccgc gaggcccaga tcctgctgga gcgcggcgcc tccgaggtcc
     5161 acctgctgga catctcctac ccggccgtgc gctacgcccg ccacgccttc ggccgcttct
     5221 accccgaccg gcggctcgcc ttctcggtga gcgacgccgg ccgcctgccc tatgccgacg
     5281 gcaccttcga cctggtgctg gtctacggct ccggccatca ctacccggac ctcgacggct
     5341 tcctctccga ggcgcggcgc gtggccggcc gggtggtcct gctggccgag cccgcccgca
     5401 tgggggcggc gcagcggctg ctcgacgcca tgggctggaa caccgagtac ggcgggctcg
     5461 acacccaccg cttcgacgag gcggagctgc gccggcgcct ggccgcccac ggcctgcgct
     5521 gcgagaccga gaggctcttc cagtacttcc ccaaggccct ggcgcggatg ggcgacgtcc
     5581 gcccgttcgt cgccgcctgg ttcggcctcc tcggcgtcct ggacgtttgc gtcccccgcg
     5641 acctgcggca ttcgctcaac gtctatgccg accggggctg aggcgccgcg ggccggcgtt
     5701 ccgggcacgg ccggcgagta cgacgccgtc cagagccggc ggcgcctcct cggctggctg
     5761 ctctacggct tcggcctgag cctgctggtc gccctcatcg tccgcaccgg cccgtggcgg
     5821 atgctcgagg cgctcctgga gagccgctgg cagccggtcg ccctcgccat cctcgccctc
     5881 gccatcggcc agaccctccg cgggatcaag tgggcgtacc tcatcggtca ggtgcagccc
     5941 ggagtcgacc gcccgctggt ggtgggctgc tacttcacca acattctggt gagctattgg
     6001 acgccggcgc gttccgggga ggccctggcg cccctcctcc tggcctcccg ctgcggcacc
     6061 cccaagcggc tggggctggc cgtcatcctg gtggaccgga tggccgacgt ggccttcctc
     6121 gccgccgccc tggtgggcag cgccctgtat ctggggtccc acgccggcag cgcggaggga
     6181 tccggcaccc tgcggacgag cctctacgcc gcggccgcct tcgccgcggt gctcggggcc
     6241 gcgggggtcg cggtgctccg ggccacccgc cccggcctgg ccgcccggca ggcgctggag
     6301 ggggacctgc ctccggagga cggcctcttc ggccgcctca ccggcctctt ctggaagctc
     6361 cgcgccgcca tcgcccaggt cgtgcggccg cgccgcctgg gggtgctgtg gatcctgacc
     6421 atggccgcct ggctcttcga cttcgccaac ctctactgga tggtcaacgc cgtcacgccc
     6481 atccgcttcg tggagagctc cgtggcccag agcgtcgccg ccggagcctc cctggcctcc
     6541 ttcgtccccg gcgggatcgg ggtggccacc gccagctatg tggtggtggc cgacctcctg
     6601 ggcgccgact ggcagcgggt ggcggcggcc ggcgtcctcg ccgtggtgct caaccaggtc
     6661 acccgcttcg ccctggcgct ggcggccggc gcccactggg gccgggccgc caagcggctg
     6721 cggtgaagcg acagttgtag tctttcatct cctgccaaac tgaaggtccc ggtcccgcgg
     6781 gctccggggt catgacggcc cggtccgtgc cgcgggcagg aggttcgaga tgtctcccaa
     6841 gatctgccgc aacgtcgtcg ccctggccgc tctcgcggcc gcgctctccg tctctccgct
     6901 tcaggccgcg ggcccgcagc tccgggaggc ccggccggaa actccctggc aggcggtcgc
     6961 cagcttctgg agcgccgcca cgcaccaggt ggtgagctgg tggaagcact cccagcagcc
     7021 ctcgccccag aagccgtcct gccagaacgg cgccatcagc tgctccaacc ccaagccccc
     7081 gggcgaggtc ggatcggcca tcgaccccga cggcaaaccc tgacctcccg gatgtctgcg
     7141 gccggggagc ggctccggcc gccccggccc ccgtccgggt ggtccgattc ttgccatcga
     7201 ggcacaccgg ccctcgccat gaaaagccgc ggggccggcg tgcggttggg cgccgcttgt
     7261 gacagaatca cattgaacgc gaggagagag agaatggaag accaccccac ccccgagtct
     7321 ctgcaggagc tcgtccgggg agggctcccg gcggagcgga cccgcgccgt ggtggcccac
     7381 ctggtccagg ggtgcggccg ctgcctcgcc gtggtggtct cccggtacca ggcgctggtg
     7441 ggcctgcccc gggtggagca cccgctcacc ccggagcagg acgccggcta cgacgccgcc
     7501 atcgaccgcg ccttcgcggc ggtgcgcgcc ttcgtcgccc gtgcccggcc ggcggcggcc
     7561 ggcggggagc ccgccgccat ggcctccccg gacagcctcc ggctcgacac cctcctcgcg
     7621 cagtccagcg ccgcgcgcta ccagagcccc gaacggatga tcgagctcgc cgaggaggcc
     7681 acgcgggtgg cggcctcgct gacctctccg ggcccgggcc tccacacgcc gcgccagatc
     7741 gccgacctcc agtgccgcgc ctgggccgag ctgggcaacg cctaccgtgt ggccgacgac
     7801 ctgggtggcg ccgaggacgc cctgggccgc gccgccgagc tgttcacccg cggcacgggg
     7861 gacgagagcc tgcgggcccg tctcttcgac cttctcgcct ctttctacgg tgcgcgccgg
     7921 cggttcgaga tggcgcgcac ggcgctggag gtggcgctgg gcatctatcg ccgccaggga
     7981 gatggccagc tcgccggccg cgcgctgctc aagctgggga cctataccgg ctacgccggc
     8041 cggccggagg aggcgatccg cctcacccgc gaggggctgg cccggctgga cgaggagcgc
     8101 gacccgggcc tcgtcttcgt cgccgtgcac aacctggccc gctggctgac cgactgcggc
     8161 cagttccgcg aggcgcgcaa gctcctctgg cagaaccggg cgcgccacga gcacgccggc
     8221 ggcgatctga acctgttgaa gatccgctgg ctcgaagggg agatcaacgc cggcctgggc
     8281 gagctggagc gggccgagaa cgcgttccgc gcggtgcacg agggcttcgc cgaggcgggc
     8341 ctgcgctaca ccgcggcggt cgccgccctc gacctcgcca cggtctggct gcgccagggc
     8401 cgggacgcgg aggcgcggca cctcgccctc gaggccgccc aggtcttcct cgtcctgcgc
     8461 atccaccgcg aggcgctggg agcggtgctg atcctccaga aagcgctgga agtgagccag
     8521 gcgagcgccg cgctcctcga gagcgtcgcc cgtttcctgc gccgcgccga ggacgacccc
     8581 accgcccgct tcgagccgaa ggcctgatta cagcgccgtc acgccggccg gcagggtgac
     8641 cggcggcggc atcgcctccc ggccgagggc gaatcgcgcc agcggctccg tctcccccag
     8701 caccacgccg gcgacgtgct cgccgagcgc cgggccgaac ttgtagccgt gcccggagcc
     8761 cccgcccacg atccaggcgt tccgcgcctc cgggtggcgg tcgatgaaga ggtggccgtc
     8821 cgggctgttc tcgtactggc agacccggga ctccacgacc ggcgccccgg cgaggcccgg
     8881 gaagcggtgg gagaggtgcc gccgcgccgc ctccagccgc tccggcgtcg gcgtccggtc
     8941 catggaggtg gggtcggtct cctcgcggcg cgtgtcgtcg gcccacttga agccccggtg
     9001 gtcgttgccc gggatgccgt agtgcaccag ctcgccgaac tcgaaccaga ccgggaaccg
     9061 cccttcgctg tacgagggat ccccggccgg cgtgccgaag tagaagacct cctgccgcgt
     9121 cggccggatc ttgccgccca cggcgtcggg gaagacgcgc ccaagccagg ggccgcaggc
     9181 gaagacatag gcatccgctt cgagatggga gccgtcggac aactccagcc gccccatccg
     9241 gcccccctcc aacgctccgg ggcgcacggc gagctgccgg tactctcccc cctcggcggc
     9301 cagcgcttcg gccaccaccc ggcacccccg gcgggccgcc aggaacccgg cctccttctc
     9361 gacgaagacc gagcggatgc cgtcgaagcg gacctggggg aagcgccgcg ccgcctcggc
     9421 gaggtccagc tccgccgccg gcagccccgc gtcgtggagc agggggaggg aggcgcgggc
     9481 atactcgtcg ctccc
//
LOCUS       DASPJI010000174         3577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_755376, whole genome shotgun sequence.
ACCESSION   DASPJI010000174 DASPJI010000000
VERSION     DASPJI010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755376"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1068)
                     /locus_tag="VEL74_04615"
     CDS             complement(64..1068)
                     /locus_tag="VEL74_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008554571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HYG61841.1"
                     /translation="MRPLALALCYDLKSDYLAAGFSPEAVMEFDDEETIAGLEDGLRQ
                     LGDRVGSVDRVGRGQALARRLAAGESWDLVFNLAEGVRGRSREAQVPALCELFDQPYT
                     FSDPLTCAVTLDKPLAKRVVRDYGLPTPAFAVVERPEDASAVTLDPPLFLKPAAEGSS
                     KGVTERSRIATRDALEETCAELIRLFRQPVLVEAYLPGREMTVGIVGNGAEARVVGVM
                     EVAASPGYEFDAYTARNKAEWEERMTYRLVADEGLAGRARQVALAAYHALGCLDAARV
                     DLRCDRSGEPNFLEINPLPGLDPVKSDLPILARLAGTSYPELLGHIVGAAAKRWGLWT
                     "
     gene            complement(1065..2231)
                     /locus_tag="VEL74_04620"
     CDS             complement(1065..2231)
                     /locus_tag="VEL74_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017741614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HYG61842.1"
                     /translation="MEQQAFASLICPETARREEICAIFGESYDPASWSDWRWQMRHRL
                     TRLDQFDKLLRLTEVERRGLLLASEKFSVAVTPHFAELIDPHDPACPIRLQVVPRDAE
                     LIVSAGDMADPCGEDSASPVEGLVHRYPDRVLFLVLDTCAAYCRYCTRSRLVSQGELE
                     PLGRRVEAVLSYLEQHTEVRDVLLSGGDPLLMSDATLDKLLGRLRAIPHIEFLRIGTR
                     IPSFLPQRITPELVQVLRRHRVWLSLHFCHPRELTPEVAEACDRLADGGVPLGCQTVL
                     LKGVNDDAETLRDLFHGLLKLRVRPYYLYQADMVLGTGHLRTSVARGLELMSQLRGHT
                     TGYAVPSYVIDGPGGGGKIPLQPETVVGHEDGVWTLRNWEGRTFTYVDPAGAEG"
     gene            2875..3312
                     /locus_tag="VEL74_04625"
     CDS             2875..3312
                     /locus_tag="VEL74_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HYG61843.1"
                     /translation="MSDHQPTIFDKILSGDIPSYKVYEDEHVLAFLDIGPLSDGHTLV
                     IPKERKTQLHQLSDEAAAAIGRVLPRLCRAVMRATGATAYNILQNNGSEAHQVVMHVH
                     FHIIPKFGNEGLGILWPAHKLADSTARDLLERMRKALDSETGR"
     gene            3309..>3577
                     /locus_tag="VEL74_04630"
     CDS             3309..>3577
                     /locus_tag="VEL74_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYG61844.1"
                     /translation="MRAAEIRGGWGLDNLVLAERPDPVAGPGQVLVRVGAVSLNYRDL
                     LTVQGVYNPKQPLPLVPCSDGAGVVEAVGPGVRRVKPGDRVCSVFA"
BASE COUNT          559 a         1276 c         1240 g          502 t
ORIGIN      
        1 tggacggatc atccttccgc gtggcctggt tgtgggcgac ggccacccgg gcgatccggt
       61 tcgtcaggtc cacagccccc accgcttggc cgcggcgccg acgatgtggc cgagcagctc
      121 cggatacgag gtcccggcga gacgcgccag gatcggcagg tccgacttga ccggatcgag
      181 gccgggcagc gggttgatct ccaggaagtt cggctcgccg gaccgatcgc agcgcaggtc
      241 gacgcgggcc gcgtccaggc agcccagggc atggtaggcg gcgagggcca cctgccgcgc
      301 ccggccggcg agcccctcgt ccgccaccag ccggtaggtc atccgctcct cccactccgc
      361 cttgttgcgg gcggtgtagg cgtcgaactc gtagccaggc gaggccgcga cctccatcac
      421 ccccaccacc cgggcctcgg caccgttgcc gacgatcccc accgtcatct cgcgtcccgg
      481 caggtaggcc tcgacgagca ccggctgccg gaacaggcgg atcagctcgg cgcacgtctc
      541 ctccagcgcg tcgcgggtgg cgatgcggga tctctccgtc acccccttgg agctcccctc
      601 ggccgccggc ttcaggaaga gcggcgggtc gagggtcacc gccgaggcgt cctccggccg
      661 ctccaccacg gcgaaggccg gggtgggcag cccgtaatcg cgcacgaccc gcttggcgag
      721 cggcttgtcc agcgtcaccg cacaggtgag cgggtcggag aaggtgtacg gctgatcgaa
      781 cagctcgcag agcgccggca cctgcgcctc gcgcgaccgc ccccgcaccc cctcggcgag
      841 gttgaacacc aggtcccagc tctcgcctgc cgcgagccgc cgggcgaggg cctgccccct
      901 gcccaccctg tccacgctcc ccacccggtc gccgagctgg cggaggccgt cctccagccc
      961 ggcgatcgtc tcctcgtcgt cgaactccat gaccgcctcc ggagagaacc ccgcggccag
     1021 gtagtccgac ttgaggtcgt agcagagggc cagcgccagc ggcctcatcc ctccgccccc
     1081 gccgggtcga cgtaggtgaa ggtcctcccc tcccagttgc ggagggtcca gaccccgtcc
     1141 tcgtggccga cgaccgtctc cggctggagc gggatcttgc cgccgccgcc agggccgtcg
     1201 atcacatagc tgggcaccgc gtagccggtg gtgtggccgc ggagctggga catcagctcc
     1261 agccccctgg cgacggacgt gcgcaggtgg ccggtgccga gcaccatgtc ggcctgatag
     1321 agatagtagg ggcgcacgcg cagcttcagc agaccatgga agaggtcgcg cagcgtctcc
     1381 gcgtcgtcgt tgaccccctt gagcagcacc gtctggcagc cgaggggcac gccaccgtcg
     1441 gccagccggt cgcaggcctc ggccacctcg ggcgtcagct cgcgcggatg acagaagtgg
     1501 agggacagcc agacccggtg ccggcgcagc acctgcacca gctccggcgt gatgcgctgc
     1561 ggcaggaagc ttgggatgcg cgtgccgatg cgcagaaact cgatgtgggg gatcgcccgc
     1621 agccgtccca gcagcttgtc gagcgtcgcg tccgacatga gcagcggatc gccgcccgac
     1681 agcaggacgt cccgcacctc ggtgtgctgc tccagatagg agagcacggc ctcgacccgc
     1741 cgtcccagcg gctcgagctc gccttggctc accagccggc tgcgggtgca gtaccggcaa
     1801 taggcggcgc aggtgtcgag caccaggaag agcacccggt ccggatagcg gtggaccaac
     1861 ccctcgaccg ggctcgccga gtcctcgccg cacggatcgg ccatgtcccc cgcgctcacg
     1921 atcagctccg cgtcccgcgg cacgacctgc agccggatgg ggcaggccgg gtcgtgtggg
     1981 tcgatgagct ccgcgaagtg cggggtcacc gcgacggaga acttctcgct cgccagcagg
     2041 aggccgcggc gttccacctc ggtcagccgg agcagcttgt cgaactggtc gaggcgggtg
     2101 aggcggtggc gcatctgcca gcgccagtcg ctccacgatg ccggatcgta gctctcgccg
     2161 aagatcgcgc agatctcctc ccggcgggcg gtctcgggac agatcagcga ggcgaacgcc
     2221 tgctgttcca tttctgcaat cctccgtaaa ggggagtcag ggttgaagaa cgcaaggtgg
     2281 tactggtatg ccgccgcacc ccggcaccca tcgaccggtc ggaaagcccc gaggggctgc
     2341 gcgcgcatgg ctcgctagtt cgctagccgg gatcggtgcg tggcggagaa ggggccgcca
     2401 aagcctgcac cggagggggg gttgtggaga cgagaaacga cgccgctgcc cggtcgcatg
     2461 cgccggacag ccgcagtgac ctcgatgccg ggagtactcg gaggttggtc aggggtgttc
     2521 acgtccactc caagataacg gagttcgaca gcatgtcaag aggtaacccg ggcttttccc
     2581 aggctgtcgt agcccaggcc cggaaccgcc ttgcgatgga ccctgcagga ggggcagccg
     2641 tcggggcgtg gagccttgtc gcgcgaggcg ggccggaccc gggaaacggc ctgaatccgg
     2701 gggctgcgga gagggaacgg gcgctggata gggttgacca cgtctcgcat ggccgacggt
     2761 gctgcaattc tgaaaccaac tttgcagcag ggcaggatcg ccgcgtgatg ccggattggc
     2821 aggtcatccg gcgggccgcc aaacccgccg cgtccctgac tacaatggcg ggccatgagc
     2881 gaccatcagc ccaccatctt cgacaagatc ctgtccggcg acatcccaag ctacaaggtg
     2941 tacgaggacg agcacgtcct cgccttcctg gacatcggcc cgctgtccga cggccacacc
     3001 ctggtgatcc ccaaggaacg caagacgcag ctccaccagc tctcggacga agcggccgcc
     3061 gccatcggcc gggtgctgcc gcgcctctgc cgcgcggtca tgcgggcgac cggcgccacc
     3121 gcctacaaca tcctccagaa caacggctcc gaggcgcacc aggtggtgat gcatgtccat
     3181 ttccacatca tccccaagtt cggcaacgag gggctcggca tcctctggcc cgcccacaag
     3241 ctcgccgact ccaccgcccg cgacctgctg gagaggatgc gcaaggcgct cgacagcgag
     3301 accggccggt gagggcggcc gagatccgcg gcggctgggg cctcgacaat ctcgtgctcg
     3361 ccgagcgccc ggacccggtg gccggccccg gccaggtgct ggtccgggtg ggcgccgtgt
     3421 cgctgaacta ccgggacctg ctgaccgtcc agggggtgta caacccgaag cagccgctcc
     3481 ccctcgtccc ctgctcggac ggcgccggcg tggtcgaggc ggtcggcccg ggcgtccggc
     3541 gggtgaagcc cggcgaccgg gtgtgcagcg tgttcgc
//
LOCUS       DASPJI010000175         4861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_583247, whole genome shotgun sequence.
ACCESSION   DASPJI010000175 DASPJI010000000
VERSION     DASPJI010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583247"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VEL74_04635"
     CDS             complement(<1..580)
                     /locus_tag="VEL74_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61845.1"
                     /translation="MNEPKRRFFSGDTLQQALVQAANHFNIDPQEIAYRSIEKKHGFL
                     KVRRKVMIEVDPDSPRRQTPAPAAPPPPRPALQAAPPPRPAAVPMSDPSDPIERSEIS
                     APPPVAPPVTPPARPERSESRPPRESRGPRGDRGPRGDRGPRSDRGPRGDRGPRSDRP
                     ARGGERRGEGRGDRGGDRPRGDRGEGRFRPSGE"
     gene            complement(618..2297)
                     /gene="yidC"
                     /locus_tag="VEL74_04640"
     CDS             complement(618..2297)
                     /gene="yidC"
                     /locus_tag="VEL74_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HYG61846.1"
                     /translation="MFLAVALSLAVLVVWNFLFPPKPPQRAPARQEAVRESPAPAPLE
                     TGAPAPATPGALATPGASGGGAARPLVQAAAEERVTLEAPGLRAVFTNRGAQLVSLEH
                     TTSSGQTLDLVRKRQQPPYPYGLVGSGLQPDPLNGALFAAEPSGDGRSVLFRYSGPLG
                     AAEKRFGFDEQGLLQVEARVPGRRDWGLLLGPGVRNPTEEELESQFEMRGAVIRSGDE
                     VTVLDAKGAEEGTALNGEGLRWVGVEDTFFLAAAIPLEGLGSVSLQPVLVRTPAGGAG
                     GAAFAPLPPKDQLTDEQEDLPREYAVVLRPEGDRLSLLSYWGAKEQDRLAALPYGLEE
                     AIQWGTLGILARPLLAGLRWIYENVVANYGWAIILMTLLIKILTLPLTHSSMVSMKKM
                     QALNPKMQAIRERYRPKLRDKQGKPNMEMQRKMNEEVMALYRSEGVNPAGGCLPLLIQ
                     MPILFAFYRMLTVAAELRGAPWLGWIQDLSIHDPYYVLPIVMGATQFLQVKLGPQAPD
                     PMQRRLFLFMPVFMTFFFLGFPSGLVLYWLTNNVLTIVQQSVYNHLRKREQ"
     gene            complement(2358..2564)
                     /gene="yidD"
                     /locus_tag="VEL74_04645"
     CDS             complement(2358..2564)
                     /gene="yidD"
                     /locus_tag="VEL74_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HYG61847.1"
                     /translation="MKRFAIFLLDLYKRRISPLLPRACRFTPTCSEYARLAILQDGLL
                     RGSARAAGRLLRCHPLHPGGVDLP"
     gene            complement(2561..2983)
                     /gene="rnpA"
                     /locus_tag="VEL74_04650"
     CDS             complement(2561..2983)
                     /gene="rnpA"
                     /locus_tag="VEL74_04650"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HYG61848.1"
                     /translation="MTEPRRSPGAPRGERLLPEERVRRRADYLRCYRTGRRRHGSLVT
                     LHFVPNQLGHPRIGITASRKVGKAVVRQRLKRRIREIYRRWQERGQLPALDLVVHLKP
                     EAGRADSAAFQAELLRLLGSVLPRRDRSRDDARGETPQ"
     gene            complement(2983..3117)
                     /gene="rpmH"
                     /locus_tag="VEL74_04655"
     CDS             complement(2983..3117)
                     /gene="rpmH"
                     /locus_tag="VEL74_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011918258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HYG61849.1"
                     /translation="MKRTFQPNNRRRKRTHGFLVRMRTRHGRAVLARRRRKGRKRLSV
                     "
     gene            complement(3269..3358)
                     /locus_tag="VEL74_04660"
     tRNA            complement(3269..3358)
                     /locus_tag="VEL74_04660"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3322..3324),aa:Ser,seq:cga)
     gene            complement(3413..3502)
                     /locus_tag="VEL74_04665"
     tRNA            complement(3413..3502)
                     /locus_tag="VEL74_04665"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3466..3468),aa:Ser,seq:gga)
     gene            complement(3516..4010)
                     /gene="tadA"
                     /locus_tag="VEL74_04670"
     CDS             complement(3516..4010)
                     /gene="tadA"
                     /locus_tag="VEL74_04670"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012772108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HYG61850.1"
                     /translation="MSERPEDRIWMMEALAEARRAADIGEVPIGCVVVAGSEVVGRGH
                     NRRETDRDPLAHAEVLAIRQAAARVGGWRLTGCTLYVTLEPCAMCAGALVNSRIDRLV
                     YGAPDPKAGYCGTLGNLVQDPRLNHRLAVTAGVLEAESAALLRGFFATLRTPRGRSPG
                     GKVL"
     gene            4239..4336
                     /gene="ffs"
                     /locus_tag="VEL74_04675"
     ncRNA           4239..4336
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VEL74_04675"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4362..>4861
                     /gene="dnaX"
                     /locus_tag="VEL74_04680"
     CDS             4362..>4861
                     /gene="dnaX"
                     /locus_tag="VEL74_04680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HYG61851.1"
                     /translation="MSYQVLARKWRPQSFSSLVGQEPIVTALRNALKEGRIAQAYLFS
                     GIRGVGKTTAARVLAKALNCEKGPAADPCNECLPCVEITRGGDLDVIEVDAATYSKVE
                     QVRDLTESLRYGPARDRYKVVVLDEVHRLSRQAFDALLKIVEEPPPHLVFVFATTEID
                     AVPATIP"
BASE COUNT          726 a         1749 c         1671 g          715 t
ORIGIN      
        1 cttcgccgga aggacggaag cgcccctcgc cacgatcgcc acggggacgg tctccgccgc
       61 gatcgccacg cccctcgccc ctgcgctcgc cgccacgggc gggccgatca ctacgcgggc
      121 cgcggtcgcc acgggggcca cggtcgctcc gcggaccacg gtcgccgcgc ggcccgcggt
      181 caccccgggg gccccggctc tcgcggggag gccggctctc gctccgctcg ggccttgccg
      241 gcggagttac cggcggggct accggcggcg gtgccgagat ctccgaccgc tcgatcggat
      301 ccgacggatc gctcatcggc accgccgccg gccggggggg cggcgcggcc tgcaacgcgg
      361 gccgtggcgg cggcggtgcc gccggggccg gcgtctggcg gcgcggcgaa tccgggtcca
      421 cctcgatcat caccttgcgc cgcaccttga ggaagccgtg cttcttctcg atgctgcggt
      481 aggcgatctc ctgcggatcg atgttgaagt ggttggcggc ctgcaccagc gcctgttgca
      541 gggtgtcgcc ggaaaagaaa cgtctcttgg gttcgttcat ggcttttggt gggcaccggt
      601 gcgtccgcgc tccggcgtca ctgctcccgc ttccgcagat ggttgtaaac gctctgttgc
      661 acgatcgtca agacgttgtt cgtcagccag tacaggacga gaccgctggg gaagcccagg
      721 aagaagaagg tcatgaagac cggcatgaac aggaacaggc ggcgctgcat cggatcgggc
      781 gcctgcgggc ccagcttcac ctggaggaac tgcgttgctc ccatcacgat cggcagcacg
      841 tagtacgggt cgtggatcga caggtcctgg atccacccca gccagggcgc gccgcgcagc
      901 tccgccgcga cggtcagcat ccggtagaag gcgaacagga tcggcatctg gatgagcagc
      961 ggcaggcacc cgcccgcggg gttgaccccc tccgaacggt agagcgccat gacctcctcg
     1021 ttcatcttgc gctgcatctc catgttcggc ttgccctgct tgtcccgcag cttcggccgg
     1081 tacctctcgc ggatcgcctg catcttcggg ttcagcgcct gcatcttctt catcgagacc
     1141 atgctgctgt gggtcagcgg cagggtcagg atcttgatca gcagggtcat caggatgatg
     1201 gcccagccgt agttcgccac cacgttctcg tagatccagc gcaggccggc cagcagcggc
     1261 cgggcgagga tgccgagcgt gccccactgg atcgcctcct cgagcccgta ggggagcgcc
     1321 gccaggcggt cctgctcctt ggcgccccag tacgacagca gcgacagccg gtcaccttcc
     1381 gggcgcagca ctacggcgta ctcgcgcggc aggtcctcct gctcgtcggt gagctggtct
     1441 ttcggcggca gaggcgcgaa cgccgcgccg ccagcgccgc cggcgggtgt acggaccagc
     1501 accggctgga gcgacacgct cccgagcccc tcgagcggga tggccgcggc caggaagaag
     1561 gtgtcctcga cgccgaccca ccgcagcccc tcgccgttga gagccgtgcc ctcctccgcc
     1621 cccttggcgt cgagcacggt cacctcgtcg cccgagcgga tcacggcgcc gcgcatctcg
     1681 aactggctct ccagctcctc ctcggtcggg ttgcggacgc cggggcccag gagcaggccc
     1741 cagtcgcgcc ggccgggcac ccgggcctcg acctggagca gcccctgctc gtcgaagccg
     1801 aagcgcttct cggcggcgcc cagcgggccg ctgtaccgga agaggacgga ccggccgtcg
     1861 cccgacggct cggcggcgaa gagggcgccg ttcagcgggt cgggctgcag gccgctgccg
     1921 accaggccgt aggggtaggg gggctgctgc cgcttccgca ccaggtccag ggtctggccg
     1981 ctggaggtgg tgtgctcgag ggagacgagc tgcgcgccgc ggttggtgaa caccgcgcgc
     2041 agcccgggcg cttcgagcgt cacccgctcc tcggccgccg cctggaccag cggccgggcc
     2101 gccccgccgc cggaagcgcc gggcgtggcc aacgcccccg gcgtggccgg agccggcgcg
     2161 ccggtctcca ggggggccgg cgccggggat tcccggaccg cctcctgccg ggccggagcc
     2221 cgctgcggcg gcttgggagg gaagaggaag ttccagacga ccaagaccgc cagcgaaagc
     2281 gcgacggcca gaaacatgcg gcgagtgtcc actgagcttc gatcctctcg atgatgttcg
     2341 tctcggcggg cccaccctta gggcaggtcg actccgccgg ggtggagcgg gtggcagcgc
     2401 aagagccggc cggcggcgcg ggcggagccg cggaggaggc catcctggag gatggccagc
     2461 cgggcgtact ccgagcaggt cggggtgaaa cggcaggcgc gggggagcag cggcgagatc
     2521 cgccgcttgt agaggtccag caggaagatg gcgaagcgct tcattgcgga gtctcccccc
     2581 gcgcatcgtc ccgcgaacgg tcacgccgcg gcaggacgct gccgaggagc cgcagcagct
     2641 cggcctggaa cgcggcggag tccgcccgtc ccgcctccgg cttcagatgg accacgaggt
     2701 ccagcgccgg gagctggccg cgctcctgcc accgccggta gatctcccgg atccgccgct
     2761 tgagacgttg gcggaccacc gccttgccga ccttccggct ggcggtgatc ccgatgcggg
     2821 gatgcccgag ctggttcggg acgaagtgga gggtcaccag agagccgtgg cgccggcgcc
     2881 cggtccggta gcacctgagg tagtccgccc gccgccgcac ccgctcttcg ggaagcaggc
     2941 gctcgcctcg gggcgcgccc ggcgagcgcc ggggttccgt catcagacgc tcaggcgctt
     3001 gcgacccttc cgacgacgcc gcgccaggac ggcgcgcccg tggcgggtgc gcatccgcac
     3061 caggaagccg tgggtccgct tgcggcgacg gttgttgggc tgaaaagtcc gtttcacgat
     3121 gaccgccttc ttcgaacgat gaacgataaa gagccgcgcg ggctgcccga tcggcacacc
     3181 cctgccgcga cacgctcgac caaaacactg aagctagacc acttgggacc cccacgtcaa
     3241 gccttgatgc cgcctgacaa ggggagaatg gcggagaggg tgggattcga acccacggac
     3301 cggttgcccg gccaacggtt ttcgagaccg ccccattcga ccgctctggc acctctccgg
     3361 gatctcgcct ttcgcggccc gccttcctac agcaggcagg ccgcatgaac gttggcggag
     3421 agagtgggat tcgaacccac ggtagagttt cccctacaca cgctttccaa gcgtgctcct
     3481 taagccactc ggacatctct ccggatctct gacttttaaa gaaccttgcc gccgggagac
     3541 cgtcccctcg gcgttcgcag cgtggcgaag aagccgcgca gcagcgccgc gctctccgcc
     3601 tccaggaccc ccgccgtcac cgccagccgg tggttgagcc gcgggtcctg gaccaggttg
     3661 cccagcgtgc cgcagtaccc ggccttggga tcgggcgccc cgtagaccag ccggtcgatc
     3721 cggctgttca ccagggcgcc ggcgcacatg gcgcacggct ccagggtgac gtagagggtg
     3781 cagccggtga gccgccaacc tccgacccgc gccgccgcct ggcggatcgc caggacctcg
     3841 gcgtgagcca gggggtcgcg gtcggtctcg cggcggttat ggccacgccc gaccacctcg
     3901 ctgccggcca ccaccacgca gccgatggga acctctccga tgtcggccgc ccgccgggct
     3961 tcggcgaggg cttccatcat ccagatccgg tcttcgggcc tctctgacac gatcctcgcc
     4021 cggcccgcct cctggcagac cgcgtatagt aaccgaaggc atccgctccg gcaaccaccc
     4081 cgccatccgc ttccattcct ctatctttgg gaatagattg gatagaagga aggttgtacc
     4141 acgaggcagg gccggagggg ccgaaacgcc cccgggcgcg gcgcctttac ttccctcacc
     4201 ccacgacgta ccatgccctc ggcagtggcc cgcgaagggt tcgggtcctg tgcatcggcc
     4261 gttaccgaac ctcgtcaggc ccggaaggga gcagcgataa ggtatccggt cgagtgccgc
     4321 agacaaaccg gaacccttcg taggccactg ccatcaccgt catgagctat caggtcctcg
     4381 cccgcaagtg gcgtccccag agcttctcga gcctggtcgg tcaggagccg atcgtgaccg
     4441 cgttgcgcaa cgccctcaag gaggggcgga ttgcgcaggc ctacctgttc tcgggcatcc
     4501 gcggcgtcgg caagacgacc gccgcccgcg tcctcgccaa ggcgctgaac tgcgagaagg
     4561 gccccgccgc cgacccctgc aacgagtgcc tcccctgcgt cgagatcacc cgcggcggcg
     4621 acctcgacgt catcgaggtg gacgccgcca cctattccaa ggtggagcag gtgcgcgacc
     4681 tgaccgagag cctgcgctac gggccggcgc gcgaccgcta caaggtggtg gtgctcgacg
     4741 aggtgcaccg cctgtcgcgc caggccttcg acgccctgct caagatcgtc gaggagccgc
     4801 cgccgcacct ggtcttcgtc ttcgccacca ccgagatcga cgccgtcccg gcgaccatcc
     4861 c
//
LOCUS       DASPJI010000176         5310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_638642, whole genome shotgun sequence.
ACCESSION   DASPJI010000176 DASPJI010000000
VERSION     DASPJI010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638642"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VEL74_04685"
     CDS             complement(<1..1016)
                     /locus_tag="VEL74_04685"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HYG61852.1"
                     /translation="MESENARRRLELETRAESGGRESLRGFLVPGLTILFHPEAERIG
                     ERVALPGLLSGREEPLSRKEPAFAHSGPGLPRPLGDLYLSRNPVLLGRGSEPGGVRVD
                     CSGTRTSVQANGVPVDGVRELTAAEVERGAVLLLAQRIVLLLSPLDPLPAPAVSDLGL
                     VGESAPMVRLRQEIRRVADLEVPVLLRGETGTGKELVARALHEAGPRRGRPYLAVNMA
                     AVPATLAAAELFGASRGAFTGADQKRLGFFARAHGGILFLDEIGETPAEVQPLLLRAL
                     ESGEIQPVGGSEPQRVDVRLIAATDADLESAVAAGRFRAPLLHRLAGYDIRIPPLRER
                     RDDFG"
     gene            1123..1434
                     /locus_tag="VEL74_04690"
     CDS             1123..1434
                     /locus_tag="VEL74_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HYG61853.1"
                     /translation="MSNELLFGLYIFVLAGFLGHHVISKVPPLLHTPLMAGTNAISGI
                     SLVGSLVAAGAEYGTLATILGFVAVTCATINTVGGFLITDRMLRMFKKRGPGGKPGGK
                     P"
     gene            1431..2822
                     /locus_tag="VEL74_04695"
     CDS             1431..2822
                     /locus_tag="VEL74_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HYG61854.1"
                     /translation="MIESLIPVSYLIASVCFILTLQLLSSPKHARLGNLLGSFGMLVA
                     VLGTLLHFEIVEYRWIAAGLILGSLIGSSMAIWIPMTKMPERIALSHCFGGLAAALVG
                     LVEYMHRIHLPGGIPRFVMAALALEVFLGFLTFTGSLMAFGKLQGLITGAPVTYKGQN
                     ATNIGLFVTALVLLVLVVANPGWGVLFYAMGVIGLAVGVLAVLPIGGADMPVVISLLN
                     SYAGLAAAATGFALGNYVLIIAGALDGTSGFLLSLKMSQAMNRSFRNVLFGAFGTGDS
                     TGVAAAGPGGAVAVNQASVEDASEVLGAAQAVIVVPGYGMAVSQAQHALRELAVLLEG
                     KGATVKYAVHPVAGRMPGHMNVLLAEANVPYDQIFDLDDINDEFARTDVVLVVGANDV
                     VNPAAKNNPGSPIYGMPVFNVDQARAVMVLKRSMNPGFAGIENELFVQPNTMMVLGDA
                     KATLRELSGALKH"
     gene            2841..3563
                     /locus_tag="VEL74_04700"
     CDS             2841..3563
                     /locus_tag="VEL74_04700"
                     /inference="COORDINATES: protein motif:HMM:NF042672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6624 domain-containing protein"
                     /protein_id="HYG61855.1"
                     /translation="MRLADRPIPWTLLLLLAVFPLVPPAAAQEPAPVTPAAPQVAPAE
                     TPVAAPWLRDELLALREEDQAARMEWIKNRADTALQEKVKAIDERTTKRVIEIIDRHG
                     WPGKALVGLDASAAAWILIQHSTPEVIDRYLPLMKEAAEKGDLSKALVALTVDRQLVY
                     HGKPQIYGSQFKAENGQWVPETLEDPANVDQRRAEVGLGPLEEYARNLRQAYGPPPPS
                     ETPKEAPKETPEPEDGSKPPHG"
     gene            3556..4329
                     /locus_tag="VEL74_04705"
     CDS             3556..4329
                     /locus_tag="VEL74_04705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG61856.1"
                     /translation="MAEPSASPAQTRIFDFREDIAYVHDAGAADFARKAAPALLEILR
                     GNGIPRGRVVDLGCGTGVWARELLQAGYRVTGVDTSRDRLLQARQTAPEAQLKRAASL
                     HDAELPPCNAVTALNEALNYVAPDFTVFPKLEDLFLRVSRALRPGGVFVFDLLVQIAG
                     RPLAFRTWETGDDWAVLVEAGENRQRRVMTRDITTFRLENDLYRRSHEVRHLAVQGRV
                     DVEWRLQKAGFAVKLLDRYGDFLLPTRRVGFQARKVREV"
     gene            4339..5196
                     /locus_tag="VEL74_04710"
     CDS             4339..5196
                     /locus_tag="VEL74_04710"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HYG61857.1"
                     /translation="MNGAELPPVLTADLFLPLNEELVALLRGLTPEDWSRPTISRGGW
                     SVREVAAHMLDTQLRRLSFQRDALPPLAPDEPIDGYAGLLAFLNGLNATWITAARRLS
                     PRVVTDLLEVSGRWQSDLFLSLDPFAPALFPVAWAGEAESRVWMDMARDLTEYWHHQQ
                     QIRLAAGAPLLLDARFTRPVLETFLRSVPRAYEGLDAAEGTRVRIVLEAPVDGTYSLV
                     RIGGPGGHWRLEPGSPSPWQASVELDPETAWRVFTRGHGREEALRRARIDGDPALGVR
                     LLDSVGMMV"
BASE COUNT          735 a         1932 c         1894 g          749 t
ORIGIN      
        1 ccgaagtcgt cgcgccgctc gcgcagcggc gggatgcgga tgtcgtagcc ggcgaggcgg
       61 tgcagcaggg gggcgcggaa gcggccggcg gcgacggcgg actccaggtc ggcgtcggtg
      121 gcggcgatga gccggacgtc cacccgttgc ggctcgctcc cccccaccgg ctggatctcc
      181 ccgctctcca gggcgcgcag cagcagcggc tgcacctcgg ccggcgtctc gccgatctcg
      241 tcgaggaaca ggatgccgcc gtgggcgcgg gcgaagaagc ccagccgctt ctggtcggcg
      301 ccggtgaagg cgccgcggga ggcgccgaac agctcggcgg cggcgagggt cgcggggacg
      361 gccgccatgt tgaccgccag gtaggggcgg ccgcggcgcg gccccgcctc gtggagggcg
      421 cgggccacca attccttgcc ggtgccggtc tcgccgcgca gcagcaccgg cacctccagg
      481 tcggccacgc ggcggatctc ctgccgcagg cggaccatgg gggcgctctc ccccaccaga
      541 ccgaggtcgg agacggcggg ggcgggcagg gggtcgagcg gggagaggag cagcacgatg
      601 cgctgcgcca ggagcaggac ggcgccgcgc tccacctcgg cggcggtcag ctcgcggacg
      661 ccgtcgaccg gcacgccgtt cgcctggacc gacgtgcggg tgccggagca gtccacccgc
      721 accccgcccg gctcgctccc ccggccgagg aggacggggt tgcggctgag gtagaggtcg
      781 cccagggggc ggggcagccc cggcccggag tgggcgaagg ccggctcctt gcgggagagg
      841 ggctcctcgc gcccggagag gaggccgggc agggccaccc gctcgccgat ccgctcggcc
      901 tcgggatgaa agaggatggt gagccccggc acgaggaagc cgcgcaggga ctcgcggccg
      961 ccgctctccg cacgcgtctc cagctcgagc cggcgccggg cgttctcgct ttccatgggc
     1021 acggaggcag tttagtcagc gcttgcggtc ccggcaaggg ccggtgtccg cgtgctagga
     1081 tacgcgccac ttgcatccga ctggcgagaa agcaggcacg tcatgtccaa cgagctgctc
     1141 ttcggtctct acatcttcgt gctggcgggg ttcctggggc accacgtcat ctccaaggtc
     1201 ccaccgctgc tccacacccc gctgatggcg gggacgaacg ccatctcggg catctccctg
     1261 gtggggtccc tggtggcggc gggggccgag tacggcaccc tggcgacgat cctcggcttc
     1321 gtggcggtca cctgcgccac catcaacacg gtgggcggct tcctgatcac cgaccgcatg
     1381 ctccgcatgt tcaagaagcg cggtcccgga ggcaagcccg gcggcaagcc atgatcgagt
     1441 ccctgatccc ggtctcctat ctgatcgcgt cggtctgttt catcctgacg ctccagctcc
     1501 tgtcgtcgcc caagcacgcg cggctgggca acctgctcgg ctcgttcggc atgctggtgg
     1561 ccgtcctcgg caccctgctg cacttcgaga tcgtcgagta ccggtggatc gccgcggggc
     1621 tgatcctggg gtccctgatc ggctcgtcga tggcgatctg gatcccgatg accaagatgc
     1681 cggagcggat cgcgctctcg cactgcttcg gcggcctggc ggcggcgctg gtcggcctgg
     1741 tcgagtacat gcaccgcatc cacctgccgg ggggcatccc gcgcttcgtc atggcggcgc
     1801 tggcgctcga ggtgttcctc ggcttcctca ccttcaccgg cagcctcatg gcgttcggca
     1861 agctgcaggg gctcatcacc ggcgcgccgg tgacctacaa gggacagaac gcgaccaaca
     1921 tcggcctctt cgtgaccgcc ctggtgctgc tggtgctggt ggtcgccaat cccgggtggg
     1981 gcgtcctctt ctatgccatg ggggtgatcg gcctggcggt cggggtgctg gcggtgctgc
     2041 cgatcggcgg cgccgacatg ccggtggtca tctcgctgct gaactcctat gccggcctcg
     2101 ccgccgcggc gaccggcttc gccctgggca actacgtgct gatcatcgcc ggcgccctgg
     2161 acggcacctc cggcttcctg ctctccctca agatgagcca ggcgatgaac cgctccttcc
     2221 ggaacgtcct gttcggcgcc ttcggcaccg gcgacagcac cggcgtggcg gccgcagggc
     2281 cgggcggggc ggtggcggtc aaccaggcgt cggtggagga cgcgtcggag gtgctgggag
     2341 cggcgcaggc ggtgatcgtg gtgcccggct acggcatggc ggtctcccag gcccagcacg
     2401 cgctccgcga gctcgccgtc ctcctcgaag gcaagggcgc cacggtgaag tacgcagtcc
     2461 atccggtggc cggccgcatg cccgggcaca tgaacgtgct gctcgccgag gccaacgtcc
     2521 cctacgacca gatcttcgac ctcgacgaca tcaatgacga gttcgcccgc accgacgtcg
     2581 tgctggtggt gggcgccaac gacgtggtca acccggcggc caagaacaac cccggcagcc
     2641 cgatctacgg catgccggtg ttcaacgtcg accaggcgcg ggcggtcatg gtgctgaagc
     2701 gcagcatgaa ccccggcttc gccggcatcg agaacgagct gttcgtccag cccaacacca
     2761 tgatggtgct gggggacgcc aaggccaccc tgcgcgagct gagcggcgcc ctcaagcact
     2821 gagcgccgga gaggagagag atgcgtctcg ccgaccgccc catcccctgg acgctgctgc
     2881 tcctcctcgc cgtcttcccc ctggtccccc ccgccgccgc ccaggagccc gctcccgtca
     2941 ccccggccgc gccacaggtg gcgcccgcgg agaccccggt cgccgccccg tggctccgcg
     3001 acgagctgct ggccctgcgc gaggaggacc aggcggcgcg catggagtgg atcaagaacc
     3061 gggccgacac cgctctccag gagaaggtga aggccatcga cgagcgcacc acgaagcggg
     3121 tcatcgagat catcgaccgg cacggctggc ccggcaaggc gctggtcggc ctggacgcca
     3181 gcgccgccgc gtggatcctg atccagcact cgacgccgga ggtcatcgac aggtacctcc
     3241 ccctgatgaa ggaggcggcg gagaagggcg acctgagcaa ggccctggtg gcgctgaccg
     3301 tcgaccggca gctcgtctat cacggcaagc cgcagatcta cggctcgcag ttcaaggccg
     3361 agaacggcca gtgggtgccc gagaccctcg aggacccggc gaacgtcgac cagcgccgcg
     3421 ccgaagtcgg gctcggcccc ctggaggagt acgcccgcaa cctccgccag gcctacgggc
     3481 cgccgccgcc cagcgagacg ccgaaggaag cgcccaagga gacgcccgag ccggaggatg
     3541 ggagcaaacc gccccatggc tgagccctcg gcctccccgg cccagaccag gatcttcgac
     3601 ttccgggaag acatcgccta cgtccacgat gccggcgccg ccgacttcgc ccggaaggcg
     3661 gccccggcgc tgctggagat cctgcgcggc aacggcatcc cccgcgggcg ggtcgtggac
     3721 ctcggctgcg gcaccggcgt ctgggcgcgc gagctgctgc aggccggcta ccgggtgacc
     3781 ggcgtcgaca cctccaggga ccggctgctg caggcgcggc agacggcacc ggaggcccag
     3841 ctcaagcggg ccgcgtcgct gcacgacgcc gagctccccc cctgcaacgc ggtgaccgcc
     3901 ctcaacgagg cgctcaacta cgtcgccccg gacttcacgg tcttccccaa gctcgaggac
     3961 ctcttcctgc gcgtctcgcg ggcgctgcgc ccgggtgggg tcttcgtctt cgacctcctc
     4021 gtccagattg ccggccggcc cctcgccttc cgcacctggg agacgggcga cgactgggcg
     4081 gtgctggtgg aggccggcga gaaccggcag cgccgggtga tgacgcgcga catcaccacc
     4141 ttccggctgg aaaacgacct ctatcgccgc tcccacgagg tccggcacct cgcggtccag
     4201 gggcgggtcg acgtggagtg gcggctgcag aaggcgggct tcgcggtcaa gctgctggac
     4261 cgctacggcg atttcctcct ccccacccgg cgggtgggct tccaggcccg caaggtccgc
     4321 gaggtctaga agcgtcccat gaacggcgcc gagctgccgc cggtgctgac ggcggacctc
     4381 ttcctccccc tcaacgaaga gctggtggcg ctgctgcgcg gcctcacgcc ggaggactgg
     4441 tcgcggccga cgatcagccg ggggggctgg agcgtccggg aggtcgccgc ccacatgctg
     4501 gacacccagc tccggcggct gtcgttccag cgcgacgccc tgcctcccct ggctccggac
     4561 gagcccatcg acggctatgc cggcctcctc gcctttctca acggcctgaa cgccacctgg
     4621 atcacggcgg cgcggcggct cagcccgcgg gtggtcaccg acctcctcga ggtgagcggc
     4681 cgctggcagt cggacctctt cctctccctg gacccgttcg cgccggcgct cttccccgtc
     4741 gcctgggccg gcgaggccga gtcccgggtc tggatggaca tggcccgcga cctgaccgag
     4801 tactggcacc accagcagca gatccgcctg gcggccggcg cgccgctcct gctcgacgcc
     4861 cgcttcaccc ggccggtcct cgagaccttc ctgcggtcgg tgccccgggc ctacgaaggg
     4921 ctggacgcgg ctgaggggac gcgggtgcgg atcgtcctgg aagcgcccgt ggacggcacc
     4981 tattctctgg tgaggatcgg cggacccggc ggacattggc ggctggagcc cgggagcccg
     5041 tccccctggc aggcgagcgt cgagctcgac ccggagaccg cttggcgggt cttcacccgc
     5101 ggccacggcc gggaggaggc gctccggcgc gcgcggatcg acggcgatcc cgccctgggg
     5161 gtgcggctgc tcgacagtgt aggaatgatg gtctgacctg agggaggaag agtccatgga
     5221 cagcctgaac cggcccccgt ccccactatc gcgccgcgcc tttgcccggc tgctcggcac
     5281 cggcgccgcc gcggcggcgt tgcggcccgc
//
LOCUS       DASPJI010000177         5715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_70656, whole genome shotgun sequence.
ACCESSION   DASPJI010000177 DASPJI010000000
VERSION     DASPJI010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70656"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VEL74_04715"
     CDS             <1..877
                     /locus_tag="VEL74_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYG61858.1"
                     /translation="GGTLFLDEIGEIPLEVQPLLLRALETGEIQRVGSDETLKADARI
                     VAATDADLEQAIGAGRFRAPLLHRLGVYEIRLPPLRERRDDVGRLLVQFLGEELHELG
                     EASRLETPKGGAPWLPASLVARLAACPWPGNIRQLRNVARQLAVDHGRSAQAQTGPQL
                     ASLLGAPEPAPRAPQPSPPPAAAGAPEPEPGRSRPERRFRSPREVTEDELVDALRTNH
                     WEPGAAARQLGISRPAVYLLIDASRRVRKASALEREELEEAFRRCGGRFQEMSDLLQV
                     SEEALKRRLKQLDIR"
     gene            1060..1632
                     /locus_tag="VEL74_04720"
     CDS             1060..1632
                     /locus_tag="VEL74_04720"
                     /inference="COORDINATES: protein motif:HMM:NF025710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HYG61859.1"
                     /translation="MNMRSLCSVSVLALALPLSAGSAWAAEATSSASARLIQPVTIIN
                     GPALNFGTLIKGNLTGPVGVTVDPQIGGFPTIVSSDPAAVTPLRGHTDANFQVTGEPD
                     EVVVISPLSSILLKKEGGGTSAREMTVSDVLIDVWEPGVPGTDLLSGNSFTMPAGAVA
                     YLEVGGKLTVEPEDELGQYTGSFVVAVNYL"
     gene            1687..2517
                     /locus_tag="VEL74_04725"
     CDS             1687..2517
                     /locus_tag="VEL74_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61860.1"
                     /translation="MSAMKTFLIASVAIFSAAVLAMPAHAGQVAIFPKRVVFSGGLRT
                     AEVTLANQGKRPVTYRIELIEQRMTPAGMLETVTAPSGPRSPIDLVRYSPRQISVAPG
                     APQTVRLLLRTPADLPPGEYRVHLLCRALPDESELADVAALAENEGLAVKLIPIPAVS
                     IPILIRHGDGLRASVRLSGLAFDPEARSLSFRLEREGGLSVYGDLTATLLPADGGPEQ
                     VIAQARGLAVYTELGALEQRLALAAGLPKGRLRLRFTSRPQDDGGGPAVSAEAETEIP
                     "
     gene            2542..5163
                     /locus_tag="VEL74_04730"
     CDS             2542..5163
                     /locus_tag="VEL74_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYG61861.1"
                     /translation="MVPRVLAVATLLLLAATGAAAQETGPEDGLLVLSVELEGRLLAG
                     DLVAFDHPEGILLPLGRLAELLELPLTVDPEGGRAQGWIAREERGASLDLERREWALG
                     NRTGRFEPALAQILEGDLYLGTPLLSSWLAIDFAVDRGALSLSVSPRERLPLQERWER
                     EESRLGLRAGGPEEGQPRFILEPYPYELAGWPTIDGSVRFALGTGEQGRTAELRPSLL
                     ARGDLLAMTAGLLVSGDEARLSLERALGGTAWRIGDVGVRPDPLVSRGHAGRGLVIDH
                     DPDPNPVASSGRTTLAGDALPGWEVELYRDAILLDFQRIGEEGRYEFRDVPLQPGLNR
                     FRLVSYGPRGERREEEERILSSADLPPAGATGWRLGIVQPGTGLLRDAAMVPSVRPDE
                     ARPGGLLGSFELERGVSRLLSWNLGLSSLALNEGRHDYLGLGLRTALPGGSGQLRFTR
                     DLAGGWAGQLQARGTFGPLQVEADQVELRGFRADGEENRTLRSRTRIWLSTQAPPLPG
                     RLFGGAGLPLSLLATRRVTAEGESLSLQAASSLSLFVGSSVVGNRWQVALADGSLSAA
                     GSLLWSGRLRGAGLRGNLSYQLAPRPVLSDLTLQAETSLGRNLAGRLGLSQGLGESRT
                     SLSAGLDWKRDGFLLGATLGVTSRGRVELGGALSFSLGRDPQAKRFRMRGAAGASQGA
                     VSARVFLDADHDGRFGPGDTPLPGVRFESGSAMPAATTGKDGIAWIQVAPNHPVDLSI
                     AEKSLADPAWVPARRGVSFVPRPGAARAVDFPLVVTGEIEGTVHLRRGDERSEASNVR
                     VQLCDAAGEVVQEVRSQFDGFYLFERVAPGRYTIRVEPGQLERLRLTARIEAPAIELR
                     SGELHRGAGIELLPNPL"
     gene            5220..>5715
                     /locus_tag="VEL74_04735"
     CDS             5220..>5715
                     /locus_tag="VEL74_04735"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYG61862.1"
                     /translation="MENIALGRRGPAAEQAEKVGPYRLEHRLGAGGMGAVWRARDERL
                     KRAVALKRILPTANEDPRLRERFRREAEAVARLNHPAIIHIYDIVETEDSDWIVMELV
                     EGWTLHELLRHEGPLDPQRVLRLGRDVAEGLAEAHSQGFIHRDLKAPNVMTTPSGRAK
                     ILDFG"
BASE COUNT          754 a         2095 c         2002 g          864 t
ORIGIN      
        1 cggcggcacg ctcttcctcg acgagatcgg cgagatccct ctcgaggtcc agcccctgct
       61 cctgcgggct ctcgagaccg gcgagatcca gcgcgtgggg agcgacgaga ccctgaaggc
      121 cgacgcgcgg atcgtcgcgg ccacggacgc cgacctcgag caggcgatcg gagcgggccg
      181 cttccgcgcg cctctcctgc accgcctcgg ggtctacgag atccggctcc cacctctgcg
      241 ggagcgccgt gacgatgtcg gccgcctgct ggtccaattc ctcggcgagg agctccacga
      301 gctcggcgag gcgtctcgcc tggagacgcc gaaaggaggg gccccgtggc tgccggcctc
      361 cctggtggcc cggctcgccg cctgcccgtg gcccggcaac atccgccaac tgcgcaacgt
      421 ggcccgccag ctcgcggtcg atcatggccg atccgcccag gcgcagaccg gcccgcagtt
      481 ggcgtcgctc ctcggcgctc ccgagcccgc cccccgggct cctcaaccgt ccccgcctcc
      541 ggctgcggcc ggcgcgcccg agccggagcc gggccgctcc cggcccgagc ggcgcttccg
      601 cagcccgcgg gaggtcaccg aggacgagct ggtggacgcc ctccggacca accactggga
      661 gcccggcgcg gccgcgcgcc agctcgggat ctcccggccc gcggtctacc tcctgatcga
      721 cgcgagccgg cgggtccgca aggcctccgc gctcgaacgc gaggagctcg aggaggcttt
      781 ccggcgctgc ggaggacgct tccaggagat gtccgacctc ctccaggtct cggaggaggc
      841 gctcaagcgc cggctcaagc agttggacat ccgctgactt cgtttctcac gtcagacgag
      901 tccgcgcccc ttctctgaca actctgacaa gccgccggcc cccgcctgcc cggggagcgt
      961 ccctgttgcg atcgttggca cgcgagttgc gaatctctcc tgtatccaat caaccacccg
     1021 gtcgtgcgaa acgatcggaa aatcatggag agatgaatca tgaacatgcg ttcgctgtgc
     1081 tcggtctccg tcctcgcgct ggcccttccg ctttccgccg gctcggcgtg ggccgccgag
     1141 gccacctcgt ccgcgtccgc gcggttgatc cagccggtca ccatcatcaa cggtcctgcg
     1201 ctcaacttcg gcaccctgat caagggaaat ctgaccggtc cggtgggcgt caccgtggac
     1261 ccgcagatcg gtgggtttcc gaccatcgtc tcgagcgacc cggccgcggt gacgccgctc
     1321 agggggcaca ccgacgccaa cttccaggtc accggcgagc ctgacgaggt ggtcgtgatc
     1381 tcgcccctgt cttcgatcct cctgaagaag gaggggggcg ggacctcggc ccgcgagatg
     1441 acggtcagcg atgtcctgat cgacgtctgg gagcccggcg tcccgggcac cgacctgctc
     1501 agcggcaaca gcttcaccat gccggcgggc gcggtggctt acctggaggt gggtggcaag
     1561 ctgacggtcg agcccgagga cgagcttggc cagtacaccg gctccttcgt ggtcgcggtg
     1621 aactacctct gatctctccg gcgccgggcg gcccgccgcc cggcgcctcc ccctcaggag
     1681 actcccatgt ccgcgatgaa aacctttctg atcgcgtcgg tggcgatctt ctcggcggct
     1741 gtcctcgcca tgccggccca cgccggccaa gttgccatct tccccaagcg cgtcgtcttc
     1801 agcggcgggc tgcgcacggc ggaggtgacc ctggccaacc aggggaagag gccggtgacc
     1861 tatcggatcg agctgatcga gcagcggatg acgcccgccg gcatgctcga gaccgtcacc
     1921 gcgccttcgg gtccccgctc gccgatcgac ctcgtgcgct attccccgcg ccagatctcg
     1981 gtggcgccgg gcgcgccgca gacggtccgg ctcctgctgc gcacgccggc ggacctcccg
     2041 cctggcgagt accgcgtcca cctgctctgc cgcgccctgc cggacgagag cgagctggcg
     2101 gacgtcgcgg ccctggccga gaacgagggg ctggcggtca agctgatccc gatccccgcg
     2161 gtctcgatcc ccatcctcat ccgccacggc gacggtctcc gcgcttccgt ccgcctgtcc
     2221 ggcctcgcct tcgaccctga ggcccggagc ctgtcgttcc gcctcgagcg cgaggggggc
     2281 ctctcggtct acggcgacct caccgcgact ctgctgcccg ctgacggcgg accggaacag
     2341 gtgatcgccc aggcccgggg gctggccgtg tacacggagc tcggagccct ggagcagcgg
     2401 ctggcgctcg ccgccggcct cccgaaaggc cggctgcggc tccgtttcac ttcgcgcccc
     2461 caggacgacg ggggaggacc ggccgtctcc gcggaagccg agaccgagat cccctgaccc
     2521 acggtccccc ggagcggcgc gatggtcccc cgtgtgctgg ccgtggccac gctcctcctc
     2581 ctggccgcca ccggggccgc cgcccaggag accgggcccg aggacggcct tctcgtcctg
     2641 agcgtcgagc tggaggggcg gctcctcgct ggcgacctcg tcgccttcga ccaccccgaa
     2701 gggatcctgt tgcctctcgg ccgactggcg gagctcctcg agctgccgct taccgtcgat
     2761 cccgagggcg gcagggccca gggatggatc gcccgggaag agcgcggcgc gtcgctcgac
     2821 ctcgaacggc gggaatgggc gctcggcaac cggaccggcc ggttcgagcc ggcgctcgct
     2881 cagatcctgg agggcgacct ctacctcggc acgccccttc tctcctcctg gctcgccatc
     2941 gacttcgccg tggaccgggg agcgctgtcg ctctcggtct cgccccggga gcggctcccg
     3001 ctccaggagc gctgggagcg ggaggagagc cggctcggcc tccgggccgg gggcccggaa
     3061 gaggggcagc cgcggttcat cctggaaccc tacccctacg agctggcggg ttggcccacg
     3121 atcgacggca gcgtgaggtt cgccctgggt accggcgagc agggccgcac ggccgagctc
     3181 cgtccgtcgc tcctcgcgcg cggcgacctg ctggcgatga cggcggggct cctggtctcc
     3241 ggcgacgagg cccggctcag cctggagcgc gcgctcggcg gcaccgcttg gcggatcggg
     3301 gacgtggggg tgcgccccga tccgctcgtc tcccgggggc acgcggggcg gggactcgtg
     3361 atcgatcacg atccagaccc gaatccagtc gcgagctccg ggcgcacgac cctggccggc
     3421 gatgcgctgc cgggatggga agtggagctg taccgcgatg ccatcctcct cgatttccag
     3481 cggatcggcg aggagggccg ctacgagttc cgcgacgtcc ctctgcagcc cgggttgaac
     3541 cggttccggc tggtgtccta cggcccgcgc ggtgaacggc gcgaggagga ggagaggatc
     3601 ctctcctcgg ctgacctgcc ccccgccggc gccacgggct ggcgcctggg catcgttcag
     3661 cccggtaccg ggctcctgcg ggatgcggcg atggtgccct ccgtgcggcc ggacgaggcc
     3721 cgtcccggag gcctcctggg gtcgttcgag ctggagcgcg gcgtttcgcg cctcctctcc
     3781 tggaacctgg ggctctcgag cctggcgctg aacgaggggc gccacgacta tctcggactc
     3841 ggcctccgca ccgcgctgcc cggcggctcg ggccaactcc gcttcacgcg cgacctggcc
     3901 ggcggctggg cgggccagct ccaggcccgg gggaccttcg ggcctctcca ggtcgaggcg
     3961 gaccaggtgg agctgcgggg ctttcgcgcc gacggagagg aaaaccgcac cttgcggagc
     4021 cgcacccgga tctggctctc cacccaggcg ccgcctctgc ccgggcggct cttcggcgga
     4081 gcggggctcc cgctctcgct gctcgccacc cggagggtga cggcggaggg ggagagcctc
     4141 tccctgcagg cagcctcttc gctctccctg ttcgtggggt cctcggtcgt cggcaaccgg
     4201 tggcaggtgg cgctcgccga cggcagtctg tcggcagcgg gcagcctgct gtggagcggc
     4261 cgtctccgcg gggccggtct gcgcggaaac ctgagctatc agctcgctcc ccggccggtc
     4321 ctctccgact tgacgctcca ggccgagacc tccctggggc ggaacctggc cgggcgcctc
     4381 ggcctgagcc agggcctcgg ggaatccagg acatcgctgt cggccggcct cgattggaag
     4441 cgggacggct tcctcctcgg cgcgaccctc ggggtgacga gccgtggccg ggtcgagctg
     4501 ggcggcgcgc tctccttcag cctcgggcgg gatcctcagg caaagcgctt ccgcatgcgg
     4561 ggggcggccg gagcctcgca gggcgcggtc tccgcgcggg tctttctcga cgccgaccac
     4621 gacggccggt tcggccccgg ggacactcct ctgcccggag tgcggttcga atccggcagc
     4681 gccatgcccg ccgccacgac cgggaaggat ggcatcgcct ggatccaggt cgccccgaac
     4741 catcccgtgg atctttcgat cgccgagaag agcctggccg acccggcctg ggtcccggcg
     4801 cggcgcggcg tctccttcgt cccccgtccc ggcgcggccc gggccgtgga cttcccccta
     4861 gtggtgaccg gcgagatcga gggcaccgtc cacctgcgcc gcggggacga gcggagcgaa
     4921 gcctccaatg tccgcgtgca gctctgcgac gccgccgggg aggtggtgca ggaggtcagg
     4981 agccaattcg acggcttcta tcttttcgag cgcgtggcgc cgggcaggta cacgatccgg
     5041 gtcgagcccg ggcaactcga acggctgcgg ctcaccgccc ggatcgaagc gcctgccatt
     5101 gagttgcgga gcggcgagct ccaccgaggc gccgggatcg agctgctgcc gaatccgctg
     5161 tgaagagtcc gagctcccgt tgacgcgcat atgccgggtt gcgctagaat cgtcgcaacg
     5221 tggaaaacat tgcattgggg aggaggggtc ctgccgcgga gcaggctgag aaggtcggcc
     5281 cctatcgtct ggagcaccgg ctcggggcgg gcggcatggg tgcggtctgg cgcgcccggg
     5341 acgagcgcct gaaacgcgcc gtcgccttga agcgcattct cccgaccgcc aacgaagatc
     5401 cgaggcttcg ggaacgtttt cgccgtgaag ccgaagccgt ggcgcgcctg aaccatccgg
     5461 ccatcatcca tatctacgac attgtcgaga cggaggacag cgactggatt gtgatggagc
     5521 tcgtggaggg gtggaccttg cacgagctcc tgcggcacga aggccccctc gatccgcagc
     5581 gcgtcctgcg cctcggccgc gacgtcgccg aagggctggc cgaagcgcat tcccaaggct
     5641 tcattcaccg ggatctcaag gcgccaaacg tgatgacgac cccctccggg cgtgccaaga
     5701 tcctcgactt cggcg
//
LOCUS       DASPJI010000178         3468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_352797, whole genome shotgun sequence.
ACCESSION   DASPJI010000178 DASPJI010000000
VERSION     DASPJI010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352797"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2078
                     /locus_tag="VEL74_04740"
     CDS             <1..2078
                     /locus_tag="VEL74_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYG61863.1"
                     /translation="CSLTNQYGTSEIHVVTSFRLPEGADGWPEIAPVGRPVANSQVYL
                     LDPGMQPLSVGVLGELYAGGACLARGYLGAPVQTAAKFVPDPFAARRGEAGARLYRTG
                     DLARQLPDGRVEWFGRIDNQVRIRGFRVELGEVETAVKQHLEVRNAAVLSLPGPGGNP
                     RLVAYVVPREGTEVPDDLRPFLKAKLPEHMVPAVFVPMAALPLNANGKLDFDALPDPG
                     KDAAETGFVAPFGPVEEMVAQILAKILNAPRVGAHDNFFELGGHSLLATQVTNRIRRN
                     FAIDLPLRTFFEGPTVSELARAVGALEAKPGRSEKIARAFLQAGSLSGAELAEKLRTS
                     RPAVAAEATPERDRQPAAESVVRLTADERAHLRRLHEGVSLDPYTHLESFVLQAKLVF
                     HQMPERLRLALLEFGRKGNLDSVLLLQDLPQDPMLPPTPTLSNQATGKTTFASELWLC
                     AVAAAFGEPVGYLQEKRGSVLQDLFPTPSNASAQSSESSAVLLAFHTEAAFHPFMPDY
                     VLLYGLRQDPHKEARTMFASVRRFLHLLSPEDQETLFTPVFRTGIDYSFGNTTGERGT
                     GPLVAVLYGDRQDPFMRYDLDLMVGQTPTACRVLEVVRDLIDQTQREVVIEPGSLVIL
                     DNRRSVHARSVFRAAFDGRDRWLQRMAVVRDLDASVGDRVRGTRVIATDFSRYLTSSD
                     RAISAGERE"
     gene            2075..>3468
                     /locus_tag="VEL74_04745"
     CDS             2075..>3468
                     /locus_tag="VEL74_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG61864.1"
                     /translation="MIDWSNADQDELSPAAREYLELLLREEGVELDSTGTIGPRGSAA
                     VELSFAQQRLWFLAQLEPGSTAYNVPAALRLSGALDVAALARSLGEVVRRHEVLRTAI
                     RTVAGQPAAEIIEMKGPFPLPLVDLSGLPPARRRGEAGGLVEREELRPFDLTHAPLLR
                     STIVVLGPAEHLALLTMHHIVSDGWSVGVLVREMGALYEAFRQGRPLPLPPLSIQYAD
                     FASWQRRELTGDTLARHLGYWRERLAGAPPVITLPTDRPRPPVRDLRGARLPVRLPSP
                     LGEAADALGQLYGATPFMTLLAMIATWLHRHGAGEDLVVGSPIANRTRAELEGLIGFF
                     ANNVVLRLGLGDNPTWRDLLARTREVTLGAYEHQDLPFEKIVEELVPARDLGYNPLFQ
                     VVVALQNTPVPALSLAGLTLGDWDLALSTARFDLVVEMEEERRWQGHIEYATDLFDAA
                     TVERMAGHLTELMRG"
BASE COUNT          496 a         1274 c         1169 g          529 t
ORIGIN      
        1 ggtgctcgct gaccaaccag tacggcacct ccgagatcca cgtggtgacc agcttccggc
       61 tgccggaggg ggccgacggc tggcccgaga tcgcaccggt cgggcgcccg gtcgccaact
      121 cgcaggtcta tctgctggac cccgggatgc agccgctctc cgtaggggtc ctgggcgagt
      181 tgtacgccgg cggcgcctgt ctagcccgcg gctacctcgg cgcgccggtg cagacggcgg
      241 cgaagttcgt ccccgatccc ttcgcggcgc ggcggggcga ggccggggca cgcctgtacc
      301 gcaccggcga cttggcgcgc cagctgccgg acggccgcgt cgagtggttc ggccgcattg
      361 acaaccaggt ccggatccgc ggcttccggg tagagctggg agaggtcgag accgcggtca
      421 agcagcacct ggaggtgcgc aatgccgccg tgctctccct gccgggcccc ggcggcaacc
      481 cccggctggt cgcctatgtc gtgccccgcg aggggaccga ggtgccggac gacctgcgcc
      541 ccttcctcaa ggcgaagctg cccgagcaca tggtgccggc ggtgttcgtg cccatggcgg
      601 ccctgccgct gaacgccaac ggcaagctcg acttcgacgc cctgcccgac cccggcaagg
      661 atgccgccga aacgggcttc gtggcaccgt tcggaccggt cgaggagatg gtcgcccaga
      721 tcctcgcgaa gatcctcaac gcgccccgcg tcggagcgca cgacaacttc tttgagctgg
      781 gcggccattc actgctcgcc acgcaggtga ccaaccggat ccggcgcaac ttcgccatcg
      841 atctgccact ccgcaccttc ttcgagggtc ccacggtgtc cgagctggcg cgcgccgtag
      901 gggccctgga agccaagccg ggccgctccg agaagatcgc ccgcgccttc ctccaggcgg
      961 gcagcctgtc gggcgccgag ctggcggaga agctccggac gagccggccg gccgtggcgg
     1021 cagaggccac cccggagaga gaccggcagc cggccgccga gtcggtggtg cgtctcaccg
     1081 ccgacgagcg cgcgcacctg cgccggctcc acgagggggt gtcgctcgat ccctacaccc
     1141 acctcgagag cttcgtcctg caggcgaagc tggtctttca ccagatgccc gaaaggctcc
     1201 gcctggccct gctcgagttc ggacgcaagg gcaatctcga cagcgtcttg ctgctccagg
     1261 acctaccgca ggatccgatg ctcccgccga cgccgactct ctccaaccag gcgaccggca
     1321 agacgacgtt cgccagcgag ctgtggctgt gtgcggtggc ggcggccttc ggcgagccgg
     1381 tgggctatct ccaggagaag cggggcagcg tcctccagga cctgttcccg accccgtcca
     1441 atgcgagcgc gcagtcgtcc gagagttcgg cggtgctgct ggccttccac accgaggccg
     1501 ccttccaccc tttcatgccg gactacgtgc tgctctacgg actgcgccag gacccgcaca
     1561 aggaggcgcg gacgatgttc gccagcgtcc gccgcttcct gcacctgctg agccccgagg
     1621 accaggagac gctgttcacc cccgtgttcc ggacgggcat cgactactcc ttcggcaaca
     1681 ccacggggga gcgcgggacc gggcccctgg tcgcggtgct ctacggcgac cggcaagatc
     1741 cgttcatgcg ctacgacctc gacctgatgg tcggccagac gccgacggcg tgccgggtcc
     1801 tggaggtcgt gcgcgacctg atcgaccaga cgcaacggga agtcgtgatc gagccgggga
     1861 gcctggtgat actcgacaac cggcgcagcg tccacgcgcg ctcggtgttc cgggccgcct
     1921 tcgacggccg ggaccgctgg ctgcagcgca tggccgtggt gcgcgacctc gatgcctcgg
     1981 tgggcgaccg ggtccgcggc acacgggtca tcgccaccga tttctccaga tatctcacca
     2041 gcagtgaccg ggcgatttcc gcaggagaac gtgaatgatc gattggagta acgccgatca
     2101 ggacgagctg tcccctgcgg cgcgcgaata tctcgagttg ctgctccgcg aggagggggt
     2161 ggagctcgac agcaccggaa cgatcggtcc gcgcggctca gccgcagtcg agctctcgtt
     2221 cgcccagcag cggctctggt tcctcgccca gctcgagccc ggaagcaccg cctacaacgt
     2281 gcccgccgcc ctgcggctct ccggggctct cgacgtcgct gcgctggcgc ggagcctggg
     2341 tgaggtggtg cggcgtcacg aggtgctgcg gaccgccatc cgcaccgtcg ctggccagcc
     2401 ggccgcggag atcatcgaga tgaaggggcc cttcccgttg cccttggtcg atctctccgg
     2461 cctcccaccg gcgcggaggc gtggcgaggc cggcgggctc gtagaacgag aggagctccg
     2521 tcccttcgac ctcacgcatg cgcccctgct gcgcagcacg atcgttgtcc tcgggcccgc
     2581 cgagcacctc gccctcctga cgatgcacca catcgtcagc gacggctggt cggtgggggt
     2641 gctggtgcgc gaaatggggg cgctctacga ggccttccga cagggacgcc ccttgccgct
     2701 gccgccgctg tcgatccagt acgccgactt cgcctcgtgg cagcggcgcg agctcaccgg
     2761 cgacacgctg gcgcgccatc tcggctactg gcgcgagcgg ctggccggcg cgccgccggt
     2821 gatcaccttg cccaccgacc ggccgcggcc cccggtgcgc gacctgcgcg gcgcccgtct
     2881 tccggtccgg ctcccctctc ctctcggcga agctgccgac gccctcggcc aattgtacgg
     2941 ggcgacgccg ttcatgaccc tgctcgcgat gatcgccacc tggctccatc gccacggcgc
     3001 cggcgaggac ctagtcgtcg gctcgcccat cgccaaccgc acgcgggccg agctcgaggg
     3061 cctgatcggg ttcttcgcca acaacgtcgt gctgcgcctc ggcctcggcg acaacccgac
     3121 ctggcgtgac ctcctcgccc gtacgcgcga ggtcaccctc ggcgcctacg agcatcagga
     3181 ccttcccttc gagaagatcg tcgaggagct ggtgcccgcc cgcgacctcg gctacaaccc
     3241 gctgttccag gtggtggtgg cgctgcagaa cacgccggtg ccggccctct ccctcgccgg
     3301 gctcaccctc ggcgactggg atctcgcgct gtccaccgcc cgcttcgatc tcgtcgtcga
     3361 gatggaggag gagcggcgct ggcaaggaca catcgaatac gccaccgacc tcttcgacgc
     3421 tgccaccgtc gaacgcatgg cgggccacct gaccgagctg atgcgggg
//
LOCUS       DASPJI010000179         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_301560, whole genome shotgun sequence.
ACCESSION   DASPJI010000179 DASPJI010000000
VERSION     DASPJI010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301560"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..526)
                     /locus_tag="VEL74_04750"
     CDS             complement(11..526)
                     /locus_tag="VEL74_04750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006633437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HYG61865.1"
                     /translation="MEIRPEQPEDVPAVHQVNSAAFGREEEARLVDLLWEAGAVVASL
                     VAVDKVGDAGEVVGHILFSPVTLDGCDRSIAGLAPMAVLPGRQRDGIGSALVERGLAA
                     CRDAGIEAVVVLGHPEYYPRFGFVPASRFGLGCEYPVPDEVFMALELTPGALAGCRGT
                     VRYRPEFSQVE"
     gene            complement(542..1117)
                     /locus_tag="VEL74_04755"
     CDS             complement(542..1117)
                     /locus_tag="VEL74_04755"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYG61866.1"
                     /translation="MRSMIRPLPSLSLVLAVLVMGLLAGSPAPASGQVPPVPKLERLP
                     EFSVVTLDGKKFAASDLRGKVVLLDFWATWCVPCLQATPHLKSLAASLAKDNFMILGI
                     SVDDDGQRLQSYVAKEKIGWPQYWDRSKMLTYRTFQISTFPTYLVVDPEGKVVYQSRG
                     WSDKVLKDLETNVRKAVQNAKQKKGGAKKAR"
     gene            1164..1952
                     /locus_tag="VEL74_04760"
     CDS             1164..1952
                     /locus_tag="VEL74_04760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HYG61867.1"
                     /translation="MQVPGYAPGNLAPIDPQDRASIAPPPRNLPPAVTAAALLAAAVF
                     PTFATWLYFVVLPGGPWALTVYAVAKGLQFSFPLVWTLWVLRVPLRLRGVEARRDLLP
                     GALSGAAILAVLFGLWRPLTTLPALGGVPVAIAAKMDAFGIVSPGAFLLLAAFYSVIH
                     SLLEEYYWRWFLLGGLRRRMSPAAALALSSLAFMSHHVLVLGEFLGGYGAATWLLALA
                     VAGAGALWSWLYLRSGALYAPWLSHACADAGLMAIGYWLWRTMG"
BASE COUNT          270 a          742 c          762 g          350 t
ORIGIN      
        1 gcgggatcga tcattccacc tggctgaatt ccggcctgta ccggacggtg ccgcggcacc
       61 ccgcgagggc tcccggcgtc agctccaggg ccatgaacac ctcgtccggc accgggtact
      121 cgcagccgag gccgaagcgg ctggcaggca cgaagccgaa gcgcgggtag tactccggat
      181 ggcccagcac caccacggcc tcgatacccg cgtcccggca ggcggcgagg ccgcgctcca
      241 ccagagccga gccgatcccg tcgcgctggc gtccggggag gacggccatc ggggcgaggc
      301 cggcgatgga gcggtcgcag ccgtcgaggg tgaccggact gaagaggatg tggccgacca
      361 cctcccccgc gtcgcccacc ttgtcgacgg cgacgaggga ggcgacgacg gcgcccgcct
      421 cccacaggag gtccaccagg cgggcctcct cctcgcgccc gaaggcggcg ctgttcacct
      481 ggtggacggc ggggacgtcc tcgggctgct ccggccggat ctccatggcg cgccgatcgc
      541 cctaccgggc cttcttcgct ccgcccttct tctgtttggc gttctggacc gccttgcgga
      601 cgttggtttc gaggtccttg aggaccttgt cggaccagcc gcggctctgg tagacgacct
      661 tgccttcggg atcgaccacc aggtaggtgg ggaaggtgct gatctggaag gtccggtagg
      721 tcagcatctt gctgcggtcc cagtactgcg gccagccgat cttctccttg gcgacgtagc
      781 tctggagcct ctggccgtcg tcgtcgacgc tgatgcccag gatcatgaag ttgtccttgg
      841 ccagcgaggc ggcgagggac ttgaggtggg gcgtcgcctg gaggcagggg acgcaccagg
      901 tggcccagaa atccaggagc accaccttgc cccgcaggtc ggaggcggca aacttcttgc
      961 cgtccagcgt gacgacggag aactcgggca ggcgctccag cttggggacc ggcggcacct
     1021 gcccggaggc gggagccggc gagccggcca gcaggcccat gaccaggacg gccaggacca
     1081 gggagaggga cgggagcggg cggatcatcg aacgcatacg gtagcctcct cgacagacgg
     1141 tactttagaa cggatggacc ccgatgcaag ttcccggcta tgctccgggc aacttggctc
     1201 ccatcgaccc ccaggaccgg gcctccatcg cgccgccgcc acggaacctc cctccggcgg
     1261 tcacggcggc ggccctgctc gcggcggcgg tcttcccgac cttcgccacc tggctctact
     1321 tcgtcgtcct cccgggcgga ccctgggcgc tgacggtcta cgcggtggcc aaggggctgc
     1381 agttctcctt cccgctggtc tggacgctgt gggtgctgcg ggtgccgctc cgcctgcgcg
     1441 gggtggaggc gcgccgcgac ctgctgccgg gggcgctctc gggggcggcg atcctcgccg
     1501 tcctcttcgg gctctggcgg ccgctcacca cgctgccggc gctcggcggc gttccggtcg
     1561 ccatcgctgc caagatggac gccttcggga tcgtctcgcc gggggcgttc ctgctcctcg
     1621 ccgccttcta ctcggtgatc cactcgctgc tggaggagta ctactggcgc tggttcctgc
     1681 tgggggggct gcgccggcgg atgtccccgg cggcggccct ggcgctgtcg agcctcgcct
     1741 tcatgtccca tcacgtgctg gtgctcggcg aattcctggg gggctacggc gccgccacct
     1801 ggctcctggc ccttgccgtc gccggggccg gcgccctgtg gtcctggctc tacctgcgca
     1861 gcggcgccct ctacgcgccg tggctcagcc acgcctgcgc cgacgccggc ctgatggcga
     1921 tcggctactg gttgtggcgg acgatgggat agtcagggct aacgcggccg gatgcagcgc
     1981 aggcggatct tgtcgtcgtc catgacgtag cagaagctcc cctcggtgtc gatggcgagc
     2041 ccctcgatct tggtctcgtc gccggcgagg tggaccggct cggtgcggat cggcccgggg
     2101 gaggcgccct ccagggtgac gcgg
//
LOCUS       DASPJI010000180         4470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_10465, whole genome shotgun sequence.
ACCESSION   DASPJI010000180 DASPJI010000000
VERSION     DASPJI010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10465"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="VEL74_04765"
     CDS             <1..164
                     /locus_tag="VEL74_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HYG61868.1"
                     /translation="HLEQARLQLTRAPRPLPRLELNPAVESLFDYRYEDISFAGYDPH
                     PAIKAPIAV"
     gene            161..679
                     /locus_tag="VEL74_04770"
     CDS             161..679
                     /locus_tag="VEL74_04770"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HYG61869.1"
                     /translation="MRVSLIVAMAAGGVIGRDNRLPWHLPADLKRFKRLTMGHPLVVG
                     RKTYESIGRPLPGRTMVVVTRDAGYAPPGVRVAHSVDEALEAAAAAEGGDEVFVAGGA
                     EIYRQTLSRADRLHLTRIDAEFPGDARFPDLDESQWRLVEEERHEPTDEVPFAYAFQM
                     FDRTGAAPGAVL"
     gene            642..1142
                     /locus_tag="VEL74_04775"
     CDS             642..1142
                     /locus_tag="VEL74_04775"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HYG61870.1"
                     /translation="MTVLALLLALSCDQRAETETSQRAAEVTDRVRVFLIAPQDGGVV
                     GQRIGCGDSAVPVEVTLPRAEPALHGAFHSLLGRRSRNDPASGYYNALYASPLELQRI
                     ERVGGEARVHLTGYLEIGDSCDGQRALAQLTETALQFQDVQRVQLWLDGKPLRDLLFK
                     SGKTAG"
     gene            complement(1152..1772)
                     /locus_tag="VEL74_04780"
     CDS             complement(1152..1772)
                     /locus_tag="VEL74_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61871.1"
                     /translation="MRHPSFTDLESSLRLAEGPERTAAVASWFQGLYAGQPEPEIPVL
                     VGEGAVELLSEGAYSADVLEFAGAIPATVSRRLKAAGFQEKDGLWSHEAGDVALEVVG
                     RRLDPQQRTTVLSPGTGDVRLLSAEDALLDLLALWQLEESPLDAITAFQFWRRLWEKL
                     DLERLGAEAARRGLLLAFERLRTFDASTVGRWPELVELQRWAGRAR"
     gene            complement(1867..2046)
                     /locus_tag="VEL74_04785"
     CDS             complement(1867..2046)
                     /locus_tag="VEL74_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61872.1"
                     /translation="MKKLILAGLVIAGALGAVQVSAPKADAAVDCSLVRCMECPTGTV
                     WAPKPNDCCRCVPVN"
     gene            complement(2398..2577)
                     /locus_tag="VEL74_04790"
     CDS             complement(2398..2577)
                     /locus_tag="VEL74_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61873.1"
                     /translation="MKRLILAGIVTLSAIGATHLTANEVEKVPLDCSSVRCLPCPEGT
                     VLEPQGNNCCRCKKI"
     gene            complement(2640..3575)
                     /locus_tag="VEL74_04795"
     CDS             complement(2640..3575)
                     /locus_tag="VEL74_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYG61874.1"
                     /translation="MNRTVCITGGAGFIGSHVADAFVSAGDRVLVLDNLSSGRRENVP
                     EGAELHVLDIRSPEAAALVREGGVDVLIHHAAQMDVRKSVEDPMFDAEVNVVGSLNLF
                     EAARHGKVRQVIFASTGGAIYGEQDFFPATEDHPARPVSPYGVSKLAAERYLFFYNVA
                     YGLDVTCLRYANVYGERQNPHGEAGVVAIFLNRLLAGQTPTINGEGKQTRDYVHVSDV
                     VRANVAATGRPGFNIYNVGTGIETSVVELYREIARAVGSDSAPQHGPAKPGEQQRSVI
                     DAGRLRADLGLPEPIPMSQGLERTAAWFRERAGRG"
     gene            3692..>4470
                     /locus_tag="VEL74_04800"
     CDS             3692..>4470
                     /locus_tag="VEL74_04800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG61875.1"
                     /translation="MRAQPLSWEGDPARVPPDRQPGPVDVVVPVYGAPEAFARCVESL
                     LAHTDLERDRLVVVLDGPQPAATMELTGRLHQQRPEGVSVLQNPARQGFVASVNRGMS
                     QSDRDVVLLNSDTRVTERWLAKLQQAAYSAPEIATVTPFSNSATICSLPRFLETNTLP
                     AGWDVDSFARLVESRSRHAYPRLPTGVGVCLYIKRKVLDDLGLFDEKSFGIGYGEESE
                     FCMRALKEGYAHVLDDATFIYHEGQRSFGSTRGHRVHDAHKA"
BASE COUNT          674 a         1550 c         1573 g          673 t
ORIGIN      
        1 accacttgga gcaggcgcgg ctgcagctca cccgcgcccc ccggccgctg ccgcggctgg
       61 agctcaaccc ggccgtcgag tcgctcttcg actatcgcta cgaggacatc tccttcgccg
      121 ggtacgatcc ccacccggcc atcaaggcac ccatcgccgt atgagggtgt cgctgatcgt
      181 ggcgatggcg gccggcggcg tcatcgggag ggacaaccgc ctaccctggc acctgccggc
      241 cgacctgaag cgcttcaagc ggctgaccat ggggcatccg ctggtggtgg gccgcaagac
      301 ctacgagtcg atcggccggc cattgccggg gaggaccatg gtggtggtga cgcgcgacgc
      361 cggctatgct ccgcccggcg tccgggtcgc ccactcggtg gacgaggccc tggaggccgc
      421 cgcggcggcc gaggggggcg acgaggtgtt cgtggccggc ggcgccgaga tctaccgtca
      481 gacgctctcc cgcgccgacc gcctccacct gacgcggatc gacgcggagt ttcccggtga
      541 cgcgcgcttc cccgatctcg acgagtcgca gtggcggctg gtcgaggagg agcgccacga
      601 gcctaccgac gaggtgccgt ttgcctatgc gtttcagatg tttgaccgta ctggcgctgc
      661 tcctggcgct gtcctgtgat cagcgggccg agaccgagac cagccagcgg gcggcggagg
      721 tcacggaccg ggtacgggtg ttcctgatcg ccccccagga cggcggcgtg gtcggccagc
      781 ggatcggctg cggcgacagc gcggtgccgg tggaggtgac gctgccgcgg gccgagccgg
      841 cgctccacgg ggcgttccat tccctgctcg gcaggcgctc ccggaacgac cccgcctcgg
      901 gctactacaa cgccctctat gcgtcccctc tcgagttgca gcggatcgag cgggtgggcg
      961 gcgaagcccg agtccacctg acgggctacc tggagatcgg cgactcctgc gacggccagc
     1021 gcgccctcgc gcagctcacc gagaccgcgc tccagttcca ggacgtccag agggtgcagc
     1081 tctggctgga cggcaagccg ttgcgcgatc tgctgttcaa gtccgggaag acggcgggct
     1141 gacggcgcgg ctcagcgcgc tctgccggcc cagcgctgca gctcgaccag ctccggccag
     1201 cgccccaccg tcgaggcgtc gaaggtgcgc aggcgctcga aggcgagcag gagcccgcgt
     1261 cgcgccgctt cggcgccgag gcgctccagg tccagctttt cccataggcg gcgccagaac
     1321 tggaaggcgg tgatggcgtc gagcggggac tcctcgagct gccagagggc cagcaggtcc
     1381 agcagggcgt cctcggcgct cagcagccgg acgtccccgg ttcccggcga caggacggtg
     1441 gtccgctgct ggggatcgag ccggcggccc accacctcca gggcgacgtc gccggcctcg
     1501 tggctccaca ggccgtcctt ctcctggaag ccggcggcct tgaggcggcg cgacaccgtc
     1561 gcgggaatcg cgccggcgaa ctccaggaca tccgcggagt acgccccctc ggagaggagc
     1621 tccaccgccc cctcccccac caggaccggg atctcgggct cgggctggcc ggcgtagagg
     1681 ccctggaacc acgaggccac cgcggccgtc ctttccgggc cctcggcgag ccgcagagat
     1741 gattccagat ccgtgaacga agggtgtctc accgggcgta ttctaatcat ctgaaaaggc
     1801 aggggaaagc ggagggtgga ccggggcgct cgctacacgc gcgagacgcc ccgatccgga
     1861 gggggctcag ttgaccggca cgcagcggca gcagtcgttc ggcttgggag cccagaccgt
     1921 gccggtaggg cactccatgc agcgcaccag cgaacagtcc acggccgcat cagccttcgg
     1981 cgcggacacc tgaacggcgc ccagggcgcc ggcgatcacc agaccagcca gaatcagctt
     2041 cttcattggg tacttctcct tgaaagtgga gagagcttct tgcctctccc gtcaaaacac
     2101 cagatcctgc tccgcaacga cgcggctcag gccttgaccc ccggccgttg ccgggatcgg
     2161 cgaggggagc cctcgggtcg aatgacttct tccggggtgc atcctctagg tgcggcgaga
     2221 gggccaaagc gggaaacccg ggctccccgt gcggcccttc tacccgctgg cttccatagc
     2281 tatcaacgaa tgagctacgg agttcccctc accctggggg attctttttc gcgggaggcg
     2341 ggaggggtgg ggagtgcgcg aaaggccggg cggcaggccc gcccggccgg agctcggtca
     2401 gatcttcttg cagcggcagc agttgttgcc ctgcggctcg agcacggtgc cctcggggca
     2461 gggcaggcag cgaaccgagg agcaatccag cgggaccttc tccacctcgt tggcggtcag
     2521 atgcgtcgcg ccgatggcgc tcagcgtcac gataccggca aggatcaatc tcttcatggc
     2581 aggtactcct tctacatctt cacggcgaga ctgcatgact cgccatgttg gggcggtttt
     2641 cagccccgac cggcgcgctc gcggaaccag gccgcggtgc gctccagacc ttgcgacatg
     2701 gggatgggct cggggaggcc gaggtcggcg cgcaggcgac cggcgtcgat gacggagcgc
     2761 tgttgctctc ccggcttggc gggaccgtgc tggggggcag agtcgctccc cacggcgcgg
     2821 gcgatctcgc ggtagagctc caccacggag gtctcgatcc cggtccccac gttgtagatg
     2881 ttgaaccccg gccggccggt ggcggcgacg ttggcgcgca ccacgtccga gacgtggacg
     2941 tagtcgcggg tctgcttgcc ctcgccgtta atggtgggcg tctgcccggc caggaggcgg
     3001 ttgaggaaga tggcgacgac gccggcctcg ccgtgggggt tctgccgctc gccgtagaca
     3061 ttggcgtacc gcaggcaggt gacgtccagg ccgtaggcga cgttatagaa gaagaggtag
     3121 cgctccgccg ccagcttgct gacgccgtag ggggagaccg ggcgcgccgg gtggtcctcg
     3181 gtggccggga agaagtcctg ctcgccgtag atggctcccc cggtcgaggc gaagatcacc
     3241 tggcggacct tgccgtgccg ggcggcctcg aaaaggttga ggctccccac gacgttgacc
     3301 tcggcgtcga acatcgggtc ctccaccgac ttgcgcacgt ccatctgcgc cgcgtggtgg
     3361 atcaggacgt ccacgccccc ttcgcgcacc agggccgcgg cctcgggcga gcggatatcc
     3421 agcacgtgca gctcggcccc ttccggaacg ttctcccggc ggccactcga caggttgtcc
     3481 agtaccagca cacggtctcc ggcggacacg aaggcgtccg cgacatgaga gccgatgaat
     3541 cctgctcctc cggtgataca gactgtacgg ttcacgcgcg cgttctcctc cctcaaagcg
     3601 ccactctcgg cgccggagct tacagcaaga gccgggccaa cgcgcctcgc atgggtctgg
     3661 cgcggcgggt gtggtagggt gccgccccag gatgagagcg caacccctgt cgtgggaggg
     3721 ggacccggcc cgggtccccc ccgaccggca gccgggaccg gtggacgtcg tcgtgccggt
     3781 ctacggcgcg ccggaggcct tcgcgcgctg cgtcgagagc ctcctcgccc acaccgatct
     3841 ggagcgcgac cggctggtgg tggtcctgga cggcccgcag cccgccgcca ccatggagct
     3901 gaccgggcgg ctccatcagc agcgcccgga gggggtctcg gtgctgcaga accctgcccg
     3961 ccaggggttc gtggccagcg tcaaccgggg gatgtcgcag tccgaccggg acgtggtgct
     4021 cctcaacagc gacacccggg tgacggagcg ctggctcgcc aagctccagc aggcggccta
     4081 ctcggcgccg gagatcgcca cggtgacgcc gttctcgaac agcgccacga tctgctcgct
     4141 cccccgcttc ctcgagacca acaccctgcc cgcgggctgg gacgtggact ccttcgcccg
     4201 gctggtcgag tcgcgctcgc gccacgccta cccgcgcctg cccaccggcg tcggcgtctg
     4261 cctctatatc aagaggaagg tgctggacga tctcggcctc ttcgacgaga agagcttcgg
     4321 catcggctac ggcgaggaga gcgagttctg catgcgggcg ttgaaggagg gctacgccca
     4381 cgtgctggac gacgccacct tcatctacca cgaggggcag cgcagcttcg gctccacccg
     4441 ggggcaccgg gtgcacgacg cccacaaggc
//
LOCUS       DASPJI010000181         6740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_472558, whole genome shotgun sequence.
ACCESSION   DASPJI010000181 DASPJI010000000
VERSION     DASPJI010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472558"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /gene="alr"
                     /locus_tag="VEL74_04805"
     CDS             <1..699
                     /gene="alr"
                     /locus_tag="VEL74_04805"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HYG61876.1"
                     /translation="FAEADDLASPRNALQEERFEEVLRLLTPDELQGVELHMANSAAT
                     LFHPESRYTLARLGLALYGLDPTQRRPRKRDRQGPEATSDLRPVMSVSARIVQLRDVP
                     AGTPLSYGGRTVTRRRSRIAVVPVGYADGYAWRLTGRAQALIRGRRVPVAGSVTMDMT
                     LLDVTDTGADLGEEVVLMGRQGREEITAVELAAAAGTISWEILCHLGLRLPRRYLRGG
                     EVEEVMSRFSREDA"
     gene            702..1457
                     /locus_tag="VEL74_04810"
     CDS             702..1457
                     /locus_tag="VEL74_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013067297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG61877.1"
                     /translation="MRLPGPVEGFFTQMGRFVQMIGRVFAWTLRPPYDWREWLRQMVR
                     VGVDSIPVVFLTTLFTGMVLALQTFTTLRRFNAENFVGSLVALSMVRELAAVLSGLIV
                     AGRAGSAMGAELGTMRVTEQIDALEVMATDPVHYLVVPRVWATTLMLPLLVGMGNAVG
                     IAGGYLVAVVLMGANPVAYVDNTFQFLELNDLFSGLIKAAVFGFLLAVIGCLQGFYTT
                     GGAEGVGRSTTSAVVVASIAILVADFFLTRLLF"
     gene            1463..2290
                     /locus_tag="VEL74_04815"
     CDS             1463..2290
                     /locus_tag="VEL74_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG61878.1"
                     /translation="MASNPSPVQIRIQGLEKSFGPKRVLDGVDVDIARGEALVIIGGS
                     GTGKSVLLKHVIGLLKPDAGTIQVDGVAVETLGNREITEFRRKFGMSFQEGALFDSMT
                     VFQNVAFPLQRLTKMSRGEVRDRVEECLAMVRLEGVGDKLPSQLSGGMRRRVGFARAV
                     AHRPEILLFDEPTTGLDPVTTALIDEVIIDLSGRLECTTLTITHDMESAFRIGDRIAM
                     IHRGKIVAEAPPEEFRRLEDPRVQQFIHGRADGPLTDETGPPAQQVGPTGGEGPRTI"
     gene            2315..3799
                     /locus_tag="VEL74_04820"
     CDS             2315..3799
                     /locus_tag="VEL74_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HYG61879.1"
                     /translation="MSNVIKVGLFATVCLIILAFLIWQIEDINPFKQEGSQLSARFAT
                     VAGLDDKAPVRVAGVRVGRVDGITLDGRQARVRLVLDQPLDLPVGTVARISSLGLLGD
                     KYIELIPGPAGGPPLPPDAVLPGTTPPSFDEAMAKLNQIGDSIQQVTGSLGGSDLGGN
                     INRLIEDVVLTSREIRLLVAENRANVASAVRNFDQVGSTLATELPRLSNQMNRTLDQI
                     ARLVEDNRGNFDQSLENVRDLTSKLQTSADNLNAISGKIASGEGTIGKLVNDDEAYNE
                     VISTLDSIQGGVETLSGTIGAISRFRIDLDMQAYVLSDEVESPGGTPSRTQTSFHLDI
                     DPQDNKRLYRVGLSSTPYGDRKEKQQTITVTNPDGSVETTTIDTLTEERSYVMTGLFG
                     YQAPRDLRLWAGIIEDSGGAQVDYPLFDRRLWLTFQAFDFGRPQDQDPHLRLQGRYQF
                     HPNLYLVGGWDDPLENNAIFLGGGIRWNDENIKYLLGAAGGALN"
     gene            complement(3962..4165)
                     /locus_tag="VEL74_04825"
     CDS             complement(3962..4165)
                     /locus_tag="VEL74_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61880.1"
                     /translation="MKRLALCLFAVVLCLTALATPTQAIPFPPAQPLSCYEACCPVGV
                     PTLGCVHEGKSYTCTTFLREYWC"
     gene            4385..4654
                     /locus_tag="VEL74_04830"
     CDS             4385..4654
                     /locus_tag="VEL74_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61881.1"
                     /translation="MAEVIWTEYLKYRAALRGFDPENIEQIVKFSQERYFDTATQRMV
                     AVGRDRHQLVMIPYEEEGETVTPVTVHATTRQQISLRLQTGRFRP"
     gene            4651..4881
                     /locus_tag="VEL74_04835"
     CDS             4651..4881
                     /locus_tag="VEL74_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002766959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HYG61882.1"
                     /translation="MRPKMSYFSEDDVLHLVISEDEEANSVELSPNITAELNEKGELI
                     GIEILKASSFVRDAILEGVQARVLNLTTATEG"
     gene            complement(4954..5850)
                     /locus_tag="VEL74_04840"
     CDS             complement(4954..5850)
                     /locus_tag="VEL74_04840"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HYG61883.1"
                     /translation="MDAILWQTDLPGLPPPRRGKVRDVYDLGDTLLIVATDRISAYDF
                     VLQPGIPGKGKVLNQLSNFWFEHLADAVPNHLLATAPSGFPESLRPYRDQLAGRAVLV
                     RKARVVPFECVARGYLAGSGFREYTADGTVCGVALPAGLQRASQLPEPIFTPATKAEQ
                     GHDMNVSFDVMAGVIGTELATRMRGLTLDLYRRGASRAAERDLILADTKFEFGLTETG
                     DLLLIDEVLTPDSSRYWDVQTWRPGEEPASFDKQYVRNWLDQTGWDHQTPPPVLADEV
                     VRGTLDRYVQAFRQITGREPEL"
     gene            complement(5896..6399)
                     /locus_tag="VEL74_04845"
     CDS             complement(5896..6399)
                     /locus_tag="VEL74_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61884.1"
                     /translation="MTDISVAAVLADLEAQMAHHRKQMELHAEQEAAHGRQREHHAAE
                     LAKLSQHYEDFKRTAGAVLEVASPRTLRSQGGDPGVPSKLSRMVDAVLAGKAADEPFS
                     ASQIAAEVRERFGARLRRPIDPRSISAKLRRMEAAGKIHQLREGRSYHEALYCRERPR
                     TERDHLS"
BASE COUNT         1081 a         2280 c         2320 g         1059 t
ORIGIN      
        1 ttcgccgagg ccgacgacct cgccagcccc cgcaacgccc tccaggagga gcgcttcgag
       61 gaggtgctgc gcctcctcac tccggacgag ctccagggcg tcgagctcca catggcgaac
      121 agcgccgcca ccctgttcca ccccgagagc cgttacactc tcgcacggct ggggctggcg
      181 ctctacgggc tcgaccccac ccagcggcgg ccgcgcaagc gggaccggca ggggccggag
      241 gccacgtcgg acctgcggcc ggtcatgtcg gtgtcggcgc gcatcgtgca gctccgcgac
      301 gtacccgcgg gcacgcccct gtcgtatggt ggacggacgg tcacccggcg ccgcagccgc
      361 atcgccgtgg tgccggtcgg gtatgccgac ggctacgcct ggcggctcac cggccgcgcc
      421 caggccctga tccgggggcg gcgggtgccg gtcgcgggct cggtcaccat ggacatgacc
      481 ctcctggacg tgaccgacac cggcgccgac ctgggggaag aggtggtgct catgggacgg
      541 caggggaggg aggagatcac cgccgtggag ctggccgccg ccgccggcac gatctcctgg
      601 gagatcctgt gccatctggg actgcggctg ccgcggcgct atctgcgcgg tggggaggtg
      661 gaagaggtga tgtcgcgatt ctcacgggag gacgcctgag cgtgcgcctc cccggccccg
      721 tcgaggggtt cttcacccag atgggccggt tcgtccagat gatcgggcgg gtcttcgcct
      781 ggacactccg gccgccgtac gactggcgcg aatggctgcg ccagatggtg cgcgtaggcg
      841 tggactccat ccccgtcgtc ttcctcacta ccctgttcac cggcatggtc ctggcgctcc
      901 agaccttcac cacgctgcgc cgcttcaacg ccgagaactt cgtcggctcg ctcgtcgccc
      961 tgtcgatggt gcgcgagctg gcggcggtgc tctccggcct gatcgtcgcc ggccgcgccg
     1021 gctcggccat gggtgccgag ctcggcacca tgcgtgtcac cgagcagatc gacgcgctcg
     1081 aggtgatggc caccgacccg gtccactacc tggtggtgcc gcgcgtctgg gcgaccaccc
     1141 tcatgctgcc gctgctcgtc ggcatgggca acgccgtcgg catcgcgggc gggtatttgg
     1201 tcgcggtggt gctgatgggg gcgaaccccg tcgcctacgt ggacaacacc ttccagttcc
     1261 tggagctgaa cgacctgttc tcgggcctca tcaaggcggc ggtcttcggc ttcctgctcg
     1321 cggtgatcgg ctgcctgcag ggcttctaca ccaccggcgg cgccgagggg gtgggccggt
     1381 ccaccacctc cgccgtggtg gtggcgagca tcgccatcct cgtcgcggac ttcttcctca
     1441 cccgtctgct gttctgagcg ccatggcgag caacccatcc cccgtgcaga tccgcatcca
     1501 gggcctggag aagtccttcg gccccaagcg ggtgctcgac ggcgtcgacg tcgacatcgc
     1561 gcgcggcgag gcgctggtga tcatcggcgg ctccggcacc ggcaagagcg tgctcctgaa
     1621 acacgtcatc ggcctgttga aacccgacgc cgggacgatc caggtggacg gcgtggcggt
     1681 cgagacgctc ggcaaccgcg agatcaccga gttccgccgg aaattcggca tgtccttcca
     1741 ggagggcgcg ctgttcgact ccatgactgt cttccagaac gtggccttcc ccttgcagcg
     1801 gctgacgaag atgagccggg gagaggtgcg cgaccgggtg gaggagtgcc tcgccatggt
     1861 gcgcctggag ggggtggggg acaagctgcc gtcgcagctc tcgggcggca tgcggcgccg
     1921 ggtcggcttc gcccgcgcgg tggcccaccg gccggagatc ctgctcttcg acgagcccac
     1981 caccggcctc gacccggtga ccacggcgct catcgacgag gtgatcatcg acctgtcggg
     2041 gcggctggag tgcacgacgc tcaccatcac ccacgacatg gagagcgcct tccgcatcgg
     2101 cgaccggatc gccatgatcc accggggtaa gatcgtcgcc gaggcgccgc cggaggagtt
     2161 ccggcggctg gaggatccac gggtccagca gttcatccac ggccgggccg acggcccgct
     2221 cacggacgag acgggcccgc cggcgcagca ggtgggaccc acgggcgggg agggcccgcg
     2281 gacgatttag ctccgcgggc ggacagggga acctatgtcg aacgtcatca aggtcggtct
     2341 tttcgcgacc gtctgtctga tcatcctggc attcctcatc tggcagatcg aggacatcaa
     2401 ccccttcaag caggagggga gccagttgag cgcccggttc gccaccgtcg ccggcctcga
     2461 cgacaaggcg ccggtgcgcg tcgccggcgt gcgcgtcggc cgggtggacg gcatcaccct
     2521 cgatggacgc caggcccgcg tgcgcctggt cctcgaccag ccgctcgacc tgccggtggg
     2581 aacggtggcg cgaatctcca gcctcggcct cctcggcgac aagtacatcg agctgatccc
     2641 cggccccgcc ggcggcccgc cgctgccgcc ggacgccgtc ctgcccggca ccaccccgcc
     2701 gagcttcgac gaggccatgg ccaagctcaa tcagatcggc gactccatcc agcaggtgac
     2761 cggctcgctc ggcggctccg acctgggggg caacatcaac cggctgatcg aggacgtggt
     2821 gctgaccagc cgcgagatcc ggctgctggt ggcggagaac cgggcgaacg tcgcctccgc
     2881 cgtgcgcaac ttcgatcagg tgggctcgac gctcgcgacc gagctgccgc gcctgtcgaa
     2941 ccagatgaac cgcacgctcg accagatcgc ccgcctggtg gaggacaacc ggggcaactt
     3001 cgatcagagc ctggagaacg tccgggactt gacgtcgaag ctgcagacct cggcggacaa
     3061 cctcaacgcc atctcgggga agatcgcgag cggcgagggg acgatcggca agctggtcaa
     3121 cgacgacgag gcctacaacg aggtgatctc gacgctcgac agcatccagg ggggcgtcga
     3181 gaccctgagc ggcaccatcg gcgccatcag ccggttccgg atcgacctcg acatgcaggc
     3241 ctacgtcctc tccgacgagg tggagagccc gggcggcacg ccgtcacgca cccagaccag
     3301 cttccatctc gacatcgacc cgcaggacaa caagcgtctc taccgggtcg gcctctccag
     3361 caccccctat ggcgaccgca aggagaagca gcagaccatc accgtcacca atccggacgg
     3421 cagcgtcgag accacgacca tcgacacgct gacggaggag cggagctacg tgatgaccgg
     3481 cctcttcggc taccaggcgc cgcgcgacct gcggctctgg gcggggatca tcgaggactc
     3541 gggaggtgcc caggtggact acccgctctt cgaccgccgt ctctggctca ccttccaggc
     3601 cttcgacttc ggccgcccgc aggaccagga cccccatctg cggctccagg ggcgctacca
     3661 gttccatccc aacctctacc tcgtgggagg ctgggacgat ccgctggaga acaacgccat
     3721 cttcctgggc ggcggcatcc gctggaacga cgagaacatc aagtacctgc tgggcgcggc
     3781 gggcggggcg ctgaactagg cgcgccctca ccccccggcc ccctctcccg cctcccctca
     3841 cccctccccg ggagaggggg aggcggacaa aatcctgagc ctgcgatggg cggtggtgcc
     3901 tccccccctg ggtggaaggg tgggagggag cgggggaggt caggaggggg gttttcctac
     3961 ctcagcacca gtactcgcgc aggaaagtcg tgcaggtgta cgacttgccc tcatgcacgc
     4021 aacccaaggt cggaactccg accgggcagc aggcttcgta gcaggatagt ggctgggccg
     4081 gcgggaaggg gatggcctga gtcggagtgg ccagggcggt cagacagagg acgacggcga
     4141 acaggcacaa cgcgagtctc ttcattccta cctccaaaga gaaggacacc ccctcatggg
     4201 gcgaccaaga ctgccgtgct gctggtcgcc gaagctagtg cagtcgatag ctcagtgtca
     4261 acaagcctcc gcgcgcccct cgtcgtttga caacccgtcc gtacgggcct aggatcgtta
     4321 aaccatggtc gctatatagt ttagctctag tccatggggc gtcttccagg atgcggggga
     4381 gtgaatggcc gaggtgatct ggacggagta cctcaaatac cgggctgcac tccgcgggtt
     4441 tgaccccgag aacatcgagc agatcgtgaa gttctcccag gagcgatact tcgacaccgc
     4501 cacgcagcgg atggtggctg ttggacgaga tcgccatcag ttggtcatga tcccttatga
     4561 agaagagggc gagacggtaa ctcccgtcac tgttcacgcc accacccggc aacagatcag
     4621 tttgcggcta cagacgggga gattcaggcc atgagaccca agatgtccta cttctctgaa
     4681 gatgatgtcc tgcacctcgt gatctccgag gacgaggaag ccaacagtgt ggagctgagc
     4741 cccaacatca cggccgagct gaatgagaag ggggagctca tcggcatcga gatcctgaag
     4801 gccagttcgt tcgtgcggga tgcgatcctc gaaggggtgc aggcgcgagt gctcaatctc
     4861 accacggcca ccgaggggtg aggaggacct gaaccccacg atcgtcatcg acagcgtcgt
     4921 cttggtgtcc tacctcctca ggtcccgccg ggcctacagc tccggctccc gccccgtgat
     4981 ctgccggaac gcctggacgt agcggtccag cgtcccccgg accacctcgt ccgcgaggac
     5041 gggcggcggt gtctggtggt cccagccggt ctggtccagc cagttgcgga cgtactgctt
     5101 atcgaaggag gcgggctcct cgcccgggcg ccaggtctgg acgtcccagt aacgcgagga
     5161 gtcgggggtg aggacctcgt cgatgagcag caggtcgccg gtctcggtga ggccgaactc
     5221 gaacttggtg tcggcgagga tgaggtcgcg ctcggcggcc cgcgaggcgc cgcgacggta
     5281 gaggtcgagg gtgaggccgc gcatccgcgt cgccagctcg gtcccgatca cccctgccat
     5341 gacgtcgaac gagacgttca tgtcgtgccc ctgctcggcc ttggtcgccg gggtgaagat
     5401 gggctccggt agctggctcg cccgctggag gcccgccggc agcgccacgc cgcacaccgt
     5461 gccgtccgcc gtgtactcgc ggaagccgct gccggcgagg tagccgcggg ccacgcactc
     5521 gaaggggacc acccgcgcct tgcgcaccag caccgcccgg ccggcgagct ggtcgcggta
     5581 ggggcgcagc gactccggga agccggacgg ggccgtggcc agcaggtggt tggggacggc
     5641 gtccgccagg tgctcgaacc agaagttcga gagctggttc agcaccttgc ccttgcccgg
     5701 gatgcccggc tgcaggacga agtcgtaggc cgagatcctg tccgtggcca cgatgagcag
     5761 cgtgtcgccc agatcgtaga cgtcgcggac cttgccgcgc cggggcggcg gcaggcccgg
     5821 caggtcggtc tgccagagga tcgcgtccat ccatctccct cccgggtccc ggggacccac
     5881 gctagaaagg gaactctatg acagatggtc gcgctctgtc cgggggcgct cgcggcagta
     5941 gagggcctcg tggtaggagc gtccttcgcg gagctggtgg atcttgccgg cggcctccat
     6001 ccggcgaagc ttggcggaga tgctcctggg atcgatgggg cggcgcaggc gcgcgccgaa
     6061 ccgctcgcgg acctcggcgg cgatctgact ggcgctgaac ggctcgtcgg ccgccttgcc
     6121 ggcgagcacg gcgtccacca tccggctcag cttggaggga acgcccgggt cgcccccctg
     6181 gcttcggagc gtccgcgggg aggccacctc gagaacggcg ccggccgtcc gcttgaagtc
     6241 ctcgtaatgc tgcgagagct tcgccagctc ggcggcatgg tgctcccgct ggcggccatg
     6301 cgccgcctcc tgctccgcgt ggagctccat ctgcttgcgg tggtgcgcca tctgcgcctc
     6361 gaggtcggcc aggaccgccg ccacggagat gtccgtcatg tctgcaacgt tactagctcc
     6421 ccgcgcctct ccgcaaggat ttttcgtcgc agagcagggt tcccgaggct ctcgacccgg
     6481 ccggaacgtc gcagacttgt gcggattcgt cgcatactcg cacagctctg cgacgaacga
     6541 acgccgctct gagacgtttg aacgctgatc tgcgacgttt gactctagct ctgcgacgaa
     6601 tgaacagggt cacgcgatga tcgggaggtg ctttgcgacg tttcggggcc gctctgcgac
     6661 gatcggcgcg ccaccgtcgc cgtgcgcgcc ggtggagaac acatcgcgcg tgaggagatc
     6721 agggaggggg cgtgggggag
//
LOCUS       DASPJI010000182         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_242030, whole genome shotgun sequence.
ACCESSION   DASPJI010000182 DASPJI010000000
VERSION     DASPJI010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242030"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2427
                     /locus_tag="VEL74_04850"
     CDS             <1..>2427
                     /locus_tag="VEL74_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG61885.1"
                     /translation="PTEATIVCTAWPVPSSSSPDRTLIGHPLEGVETLVADRYGEPVL
                     LGAPGELWIGGSGVARGYLHRDELTAERFVEKEGRRWYRTGDLVRQLPGRGGVLEFLG
                     RTDQQVKVRGFRVEPGEIETILVSHPRIREAVVAARDAGQGDRRLVAWYVPAGEDPGA
                     AAVRDFLAARLPGYMVPSLFAALPELPLTTHGKVDRDRLPEPEAATAATVDGPHEAPR
                     TPAEELVAGVWSEILGVPSVGRTADFFDLGGHSLLATRVVTRLREGLGIDLLVRSLFQ
                     APTVEALARELEALLAAGDGETAPPIVPVPREGDLPLSFAQERLWLVERLTPGTAAYN
                     IPLALEVEGAASPAALEAALGEMVRRHEVLRTTYEEHERLPVQRIAPAAPWTLPLVDF
                     SGLPAASREKEARLLAEEEVSRPFDLEQGPVLRTTLLRLGADRHALLLNVHHIAADGW
                     SLEVMLAEIAALYQGETLPEVPVQYADFAVWQRRWLSGDALERQLAYWRDRLRSSPAL
                     DLPTDRPRPAQQSFRGATLDVPLPPEALLGLQELARRQDATLFMVVLAVFQTLLSRYT
                     GQDDIVLGSPIANRNRSEVEPLIGFFVNTLVLRGDLAGDPPFTELVARARQTALEAYG
                     HQDLPFGRLVEELRPERRLSHNPLFQVMVGVQNAPAGRVELPGLSFAAADFDLAVTRF
                     DLEAMIWETGEAPVAQLTWATDLFDAVTVGRMARHLETLLAGLIEDPERRLSDLPLLG
                     FPERHQLLREWNDTAADLPEVTLVDLFREQAARRPGAVAVSSAEGDLTYADLDIRSDR
                     LARRLAAAGV"
BASE COUNT          292 a          909 c          858 g          368 t
ORIGIN      
        1 gccccaccga ggcgaccatc gtctgcaccg cctggcccgt gcccagctcc tcctcccctg
       61 accgcaccct catcggtcat ccactggagg gtgtcgaaac gctggtggcg gaccgctacg
      121 gcgagccggt cctcctgggc gctcccggcg agctgtggat cggcggttcc ggggtggccc
      181 gcggctatct ccaccgcgac gagctgaccg ccgagcgctt cgtcgagaag gaaggccgcc
      241 gctggtatcg cacgggcgac ctcgtccgcc agctccccgg gcgggggggc gtgctggagt
      301 tcctcggccg caccgaccag caggtgaagg tgcgcggctt ccgcgtcgag ccgggggaga
      361 tcgagaccat ccttgtctct catccccgga ttcgcgaggc cgtggtggcc gcccgcgacg
      421 ccggtcaggg tgaccggcgc ctggtggcct ggtacgtgcc cgccggcgag gaccccggcg
      481 ccgcggcagt gcgcgacttc ctggccgccc gcttgccggg ctacatggtg ccgtcgctct
      541 tcgcggccct ccccgagctg cctctgacca ctcacggcaa ggtcgatcgc gaccggctcc
      601 ccgagcccga agcggccacc gccgccaccg tggacggtcc gcacgaggcg ccgcgcacgc
      661 cggccgagga gctggtcgcc ggggtctggt ccgagatcct gggggtcccc tccgtcggcc
      721 ggaccgccga cttcttcgat ctgggcggac actccctgct cgccacccgg gtggtgacgc
      781 ggctgcggga ggggctggga atcgacctgc tggtgcggag cctcttccag gcaccgacgg
      841 tggaggcgct ggcccgggag ttggaggccc tcctggcggc tggtgacggc gagacagcgc
      901 cgcccatcgt gccggtgccc cgcgaagggg acctccctct ctcgttcgcc caggagcggc
      961 tctggctcgt cgagcgactg actcccggca ccgcggccta caacatcccc ctggccctgg
     1021 aagtggaggg cgctgcctcg ccggccgccc tggaggccgc gctcggcgag atggtgcggc
     1081 gccacgaggt gctgcgtacg acctacgagg agcacgagag gctcccggta cagcgcatcg
     1141 cgccggccgc cccctggacg ttgccgctag tggacttctc tggcctgcct gcggcgagcc
     1201 gggagaagga ggcccgtctc ctggccgaag aggaggtgtc tcgccccttc gacctcgaac
     1261 aggggccggt cctccggacc actctgctgc gcctgggggc ggaccgccac gcgctgctgc
     1321 tcaacgtcca tcacatcgcc gccgacggct ggtcgctgga ggtgatgctg gcggagatcg
     1381 ccgccctcta tcagggtgag acgctgcccg aggtgcccgt ccagtacgcc gacttcgcgg
     1441 tctggcagcg gcgctggctc tccggcgacg ccctggagcg gcagctcgcc tactggcgcg
     1501 accggttgcg aagctctccg gccctcgacc tgcccaccga ccggccgcgg ccggcgcagc
     1561 agagcttccg cggcgccacg cttgacgtgc ccctgcctcc agaggctctt ctgggtctcc
     1621 aggagctggc gcgccggcag gacgccaccc tcttcatggt ggtcctcgcc gtcttccaga
     1681 cgctgctctc ccgttacacc ggccaggacg acatcgtgct gggctcaccg atcgccaacc
     1741 gcaaccggtc cgaggtggag ccgctcatcg gcttcttcgt caacaccctg gtgctgcgcg
     1801 gcgatctcgc cggcgatccg ccgttcaccg agctggtggc gcgggcgcgg cagaccgcgc
     1861 tggaggccta cggccaccag gacctcccct tcggccggct ggtggaggag ctgcggccgg
     1921 agcggcggct ctcccacaac ccgctgttcc aggtcatggt cggggtgcag aacgctccgg
     1981 ccggccgcgt cgagctgccg gggctgtcct tcgccgccgc cgatttcgat ctcgcggtga
     2041 cccgcttcga cctggaggcg atgatctggg agaccgggga ggcgccggtg gcccaattga
     2101 cctgggcgac cgatctcttc gacgccgtta ccgtcggccg catggcgcgc catctggaga
     2161 ccctgctggc cggactgatc gaggacccgg agcggcgact gtccgacctg cccctgctgg
     2221 gcttccccga gcggcaccag ctcctccggg agtggaacga caccgcggcg gacctgcccg
     2281 aggtgaccct ggtcgatctc ttccgggagc aggccgcccg ccggccgggg gcggtggcgg
     2341 tctcgtcggc ggagggtgac ctcacctacg cggacctcga catccgttcg gaccggctgg
     2401 cccggcgcct cgccgccgcg ggcgtcg
//
LOCUS       DASPJI010000183         5747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_301844, whole genome shotgun sequence.
ACCESSION   DASPJI010000183 DASPJI010000000
VERSION     DASPJI010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301844"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1556
                     /locus_tag="VEL74_04855"
     CDS             <1..1556
                     /locus_tag="VEL74_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HYG61886.1"
                     /translation="LLDGVTITGNTASFDGGGLWADGFEMDLTIRNTIISGNTAEGNG
                     GGIYVEDTGGPLLIQSSVISGNTADGIGGGIYFYDPDDTVTIEDSTISGNTAVGGGGV
                     FLYDTDGGGVTIRRTTIDGNSAALGGGLLMFHPDHPVLLENSTISGNQALGGGGLFLY
                     GLYGNASLTLRSTTVAGNTAYYGGGLLTLTTVTIENSIVGDNVAQESPDLGTAEGGAV
                     DLAFSLVETPGTANINDNGGNIFDQDPQLGPLQNNGGPTATHLPAATSPAVDAGDPAF
                     APPPVTDQRGLPRVAGGRIDMGSVETEEPVVINAGTIQLAVTAASINENGGTITITAT
                     RTGGSDGAVSVSYTTSDGTAVAPGDYTGAAGTLNWADGDAAPKTFQVTIINDTTDEPD
                     ETFNVTLSNPQGGATLGADTTEVVTILDDDVQSNVVEIPTVGDYGRMLLVGLCALSGF
                     LMLRRRKGFAAPMLAVSLALGAAGFEGAPASQPEVLEVRQGANGEIKKVRIEVVDTPA
                     QARTLAGQE"
     gene            1710..2162
                     /locus_tag="VEL74_04860"
     CDS             1710..2162
                     /locus_tag="VEL74_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61887.1"
                     /translation="MTNFPEVQQITGRVVISEPIPQTRFETVKAVVAPASPADAAQWT
                     EAGVIDTRGFTHLTLSLAGTLQGSAQAGAIGVALVPDVPELLNALRTHGAAPFALRAE
                     AEVTAPLGGFFSSGSTTVRIGFPRYRVFLYNATSKSAEALVYAYLSGS"
     gene            2159..2716
                     /locus_tag="VEL74_04865"
     CDS             2159..2716
                     /locus_tag="VEL74_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61888.1"
                     /translation="MMSLRALIAVLITFSGAPLLAAEQGKPAAAPAVRLFTTAQLAGW
                     LCKALPSNDGRKLAATLFDGKLPGEETDKGYWQVSLMEDGKDLIDVNFMGMELMEDVA
                     SWVLTLELEGNWHGISEADWLALAQRVRGRTEKGDHRGTKICAAEKKSCRQLGGYEVG
                     PGVRWLQLVWGSSDPNPSTWFCAKG"
     gene            complement(2720..3688)
                     /locus_tag="VEL74_04870"
     CDS             complement(2720..3688)
                     /locus_tag="VEL74_04870"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYG61889.1"
                     /translation="MPPELPSHVGPYRILELLDRGGTSPVFKAEDPAHDRIVAVKLLS
                     PRLLEDPVARERFERETDSMVRLGHSNVVQTFEAGRDADRPYLVREYLEGTSLDRLLR
                     QRRLTTAEAFHVMRAICRGLAHAHQNGVVHRSLTPRDILVSPDLSVVKITDFGRIDLL
                     TSMTGTLNTGAISLGAFHYLAPEQVEAEPGKDRVDHRADLYAAGVIFHEMLTGRAPGG
                     RFVLPSQLNSELPPEVDVLVFRCLEKNPAERYGNALELLADLTRLEETLRLRLASELK
                     GITQAGSKLLAGGPEGQAAGNRRTALIVTVLLILVVAAVAIYLIAR"
     gene            complement(3678..4991)
                     /locus_tag="VEL74_04875"
     CDS             complement(3678..4991)
                     /locus_tag="VEL74_04875"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HYG61890.1"
                     /translation="MVNALALAAGIAVFAVLVALIAWAGRRSFFVRQLAFPLHVAAAA
                     CSLGAVRLLQPLGLGTPELHKSLVLVLDGTLIFLGIVLVLRLLGLYLFDVHLTARRGM
                     RLPPLLPAVTMAVAYLITALITLRLTFPQFDIRPLLATSAVTSLVLGLALQPLLGNFF
                     AGLVISIEKPFRINDWIRVGDLEGRVVAITWRTTHLRTRENDNLVIPNGKLADERVLN
                     YYYPHPMHMERVQVGADYKVPPYRVRRALLDCVSGVPGALDKPTPDVFILRFDENAIV
                     YELRVWVEDMVQAPRIASDLRGRIWEEFQRAGIIIPTPIRTLHLAPRPRRERGAPEDG
                     PWPARLYIAEGPDRGRSVALAAPPAPPAVVGRSRVCTLVLSDPNASKEHLRIAWEDGA
                     WVLTDLGSSFGTQVNGEPADRVALRPLDRVTVGDTVMIVEKSDAP"
     gene            5164..5742
                     /locus_tag="VEL74_04880"
     CDS             5164..5742
                     /locus_tag="VEL74_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYG61891.1"
                     /translation="MKRAYVCGMALALLALPGAAVAHDHGEKPATAAGAAAGGAEVRA
                     EILRQLGDAEEKLMALAEAMPAEKYGWRPAEGVRTVGEVFLHVAGGNYFLPTFWGVKP
                     PEGIDMRAIEKDGADKAKVIAGLKRSFDHARKAIADVPEAEMGKAIKIFGRDGSVREA
                     FMIDATHAHEHLGQAIAYARMNGIAPPWSRGE"
BASE COUNT          955 a         1930 c         2031 g          831 t
ORIGIN      
        1 tgctcctcga cggcgtcacc atcaccggca acacggccag cttcgacggc ggcggcctct
       61 gggccgacgg cttcgagatg gacctgacca tccggaacac catcatctcc ggcaataccg
      121 cggagggtaa cggtggcggc atctacgtgg aggacaccgg cggaccgctg ctcatccaga
      181 gctcggtgat ctcgggcaat acagccgacg gcatcggcgg cggcatctac ttctacgatc
      241 ccgacgacac ggttacgatc gaggactcca ctatctccgg caacaccgcc gtcgggggcg
      301 gcggtgtgtt tctctatgac acggacggtg gcggggtcac catccgccgc acgacgatcg
      361 acggcaactc cgccgcgctc ggcggtggcc tcctcatgtt ccacccggac catcccgtcc
      421 tcctggagaa cagcacgatc tcgggaaacc aggcgctcgg cggcggtggc ctcttcctct
      481 acggcctcta cggcaatgcc tccctgacgc tgcgctcgac gacggtggcg ggcaacacgg
      541 cctactacgg tggcggcctt ctcaccctga ccaccgtcac catcgaaaac tcgatcgtgg
      601 gcgacaacgt ggcgcaggaa agcccggatc tgggcactgc cgaaggcggc gcggtcgatc
      661 tcgccttcag cctggtggag accccgggga cggccaacat caacgacaac ggcggcaaca
      721 tctttgacca ggacccgcaa ctcggtccgc tgcagaacaa cggcggcccg accgcgaccc
      781 acctgccggc ggcgaccagc ccggcggtcg acgccggcga cccggccttc gcgccgccgc
      841 cggtcaccga ccagcgcggc ttgccccggg tggcgggcgg ccggatcgac atggggtccg
      901 tcgaaacgga agagcccgtg gtgatcaacg cggggacgat ccagcttgcg gtcaccgccg
      961 cctccatcaa cgagaacggc ggcacgatca ccatcaccgc cacacgtacc ggcggcagcg
     1021 acggcgcggt gtcggtctcg tacacgacga gcgacggcac ggcggtggcg cccggcgact
     1081 acaccggtgc cgccggcacc ctcaattggg ccgatggcga cgcggcaccg aagacgttcc
     1141 aggtaacgat catcaacgac accaccgacg agccggacga gaccttcaac gtgaccctct
     1201 cgaacccgca gggcggcgcc accctcggcg ccgacaccac cgaggtggtg accatcctgg
     1261 atgacgacgt gcagagcaac gtggtggaga tccccaccgt gggggactac ggccggatgc
     1321 tcctcgtcgg cctgtgcgcg ctgagcggtt tcctgatgct gcgccggagg aagggcttcg
     1381 ccgccccgat gctggccgtc tccctggctc tcggcgcggc cgggttcgag ggagctcccg
     1441 cgagccagcc ggaggtgctc gaggtccgcc agggcgcgaa cggcgagatc aagaaggtgc
     1501 ggatcgaggt cgtggacacc ccggcgcagg cccggaccct ggccgggcag gagtagaagc
     1561 acggggaggc tgcccgagag cgcttccttc aagggcgctc tcgcgcctac actgctccca
     1621 tgcaccatct gagaaccgct ttttcgctct gtaccgtcgt ttccctttgc gcagtggctt
     1681 cgtcctgggc ccagggcgag ccggtagtcg tgacgaactt cccggaggtc cagcagatca
     1741 ccggccgggt cgtcatctcc gagccgatcc cgcagacccg cttcgagacc gtgaaggccg
     1801 tggtcgcgcc tgccagtccg gccgatgccg cccaatggac cgaagccggg gtcatcgata
     1861 cccggggatt cacccatttg accctcagcc tggccggcac cctgcagggc agcgcgcagg
     1921 ccggggccat cggagtggcg ctcgttcccg atgtcccgga gctgctgaac gccctgcgga
     1981 cccacggagc cgcacccttc gccctgcgcg ccgaggccga ggtcacggcg cccctgggcg
     2041 gcttcttcag ctcggggtcc acgaccgttc gtatcggttt tcctcgctat cgcgtcttcc
     2101 tttacaacgc cacctctaaa tccgcggagg cgttggtcta cgcgtacctg agtggctcat
     2161 gatgtccctg agagcgctca ttgctgttct gatcactttc tccggcgctc cgctgctcgc
     2221 cgcggagcaa gggaagcccg cggcggctcc cgccgtccgg ctgttcacca ccgcccaact
     2281 cgccggctgg ctctgcaagg ccctgccctc caacgatggc cggaagctcg cggccacgct
     2341 cttcgacggc aagctgccgg gagaggagac ggacaaagga tattggcagg tgtcgctgat
     2401 ggaagatgga aaggatctga tcgacgtcaa cttcatgggc atggagctga tggaagacgt
     2461 cgcctcctgg gtgttgaccc tcgagctcga agggaactgg cacggcatct cggaggcgga
     2521 ctggctggca ctcgcgcagc gggtccgcgg gcggaccgaa aagggagatc accgcggcac
     2581 caagatctgc gccgccgaga agaagagctg tcggcagctc ggcggctatg aggtggggcc
     2641 cggcgtccgc tggctgcaac tggtctgggg gagctccgac cccaacccct cgacctggtt
     2701 ctgcgcgaag ggctgagagt cagcgagcaa tcagataaat cgccaccgcg gccaccacca
     2761 ggatcagcag aacagtgacg atcagggccg tccggcgatt gccggcggcc tgcccctccg
     2821 gccctcccgc caggagcttg gagccggcct gggtgatgcc cttgagctcg gaggcgaggc
     2881 gcaggcgcag cgtctcctcc agccgggtga ggtcggccag cagctccagg gcgttgccgt
     2941 agcgctcggc cgggtttttc tccaaacagc ggaacaccag cacgtccacc tccggcggca
     3001 gctcgctgtt gagctggctg ggcaggacga acctgccccc tggcgcccgg ccggtgagca
     3061 tctcgtggaa gatcaccccc gcggcgtaga ggtcggcccg gtggtcgacg cggtccttgc
     3121 cgggctcggc ctccacctgc tccggcgcca gatagtggaa ggccccgagc gagatggcgc
     3181 cggtgttgag cgtgccggtc atgctggtca gcaggtcgat acgcccgaag tcggtgatct
     3241 tcaccaccga gaggtctgga gagaccagga tgtcgcgcgg ggtgaggctg cggtggacca
     3301 ccccgttctg gtgggcgtgg gcgaggccgc ggcagatggc gcgcatgacg tggaacgcct
     3361 cggccgtggt gagccgccgc tggcgcagca gccggtcgag cgaggtgccc tccaggtact
     3421 cgcgcaccag gtaggggcgg tcggcgtcgc ggccggcttc gaaggtctgt accacgttcg
     3481 aatgccccag gcgcaccatg gagtcggtct cgcgctcgaa gcgctcgcgt gccaccgggt
     3541 cttccagcag ccgcggggag aggagcttga ccgccacgat gcggtcgtgg gccgggtcct
     3601 cggccttgaa gaccggggac gtgccgccgc ggtccagcag ctccaggatg cggtagggac
     3661 cgacgtggga ggggagctca gggggcatcg cttttctcca cgatcataac cgtatcgccc
     3721 accgtgacgc gatcgagcgg ccggagggcc acgcggtccg ccggctcgcc gttgacctgc
     3781 gtgccgaagc tgctgcccag gtcggtgagc acccaggcgc cgtcctccca ggcgatccgc
     3841 aggtgctcct tgctggcgtt gggatcggag agcaccagag tgcacacccg ggagcggccc
     3901 accacggccg gtggcgccgg gggagccgcg agcgcgaccg agcgcccgcg gtccggcccc
     3961 tcggcgatgt agagccgggc tggccagggg ccgtcctcgg gcgccccgcg ctctcttcgg
     4021 ggacggggag cgaggtgcaa cgtccggatc ggggtgggga tgatgatccc cgcgcgctgg
     4081 aactcctccc agatcctccc ccgcaggtcg ctggcgatgc gcggcgcctg gaccatgtcc
     4141 tcgacccaca cccgcagctc gtagacgatg gcgttctcgt cgaagcggag gatgaagacg
     4201 tccggcgtgg gcttgtccag cgccccgggc acgccggaga cgcagtcgag aagggcccgg
     4261 cgtacgcgat aagggggcac cttgtaatcg gccccgacct gcacccgctc catgtgcatg
     4321 gggtgcgggt agtagtagtt gagcacccgc tcgtcggcca gcttgccgtt ggggatcacc
     4381 aggttgtcgt tctcgcgcgt ccgcaggtgg gtggtgcgcc aggtgatggc cacgacccga
     4441 ccttccaggt cgcccacccg gatccagtcg ttgatgcgga acggcttctc gatgctgatc
     4501 accagcccgg cgaagaagtt gccgagcagg ggctggaggg ccaggccgag caccagggag
     4561 gtgacggcgc tggtggcgag cagcggccgg atgtcgaact gcgggaaggt gagccgcaag
     4621 gtgatcagcg cggtgatcag gtaggccacc gccatggtga ccgccggcag cagcggcggc
     4681 aggcgcatcc ccctgcgggc cgtcaggtgg acgtcgaaca ggtagaggcc cagcaggcgc
     4741 agcaccagga cgatgccgag gaagatcagc gtaccgtcca ggacgagcac cagggacttg
     4801 tggagctccg gggtgcccag gccgaggggc tgcagcagcc ggaccgctcc cagggagcag
     4861 gccgccgccg ccacatggag cgggaaggcg agctggcgga cgaagaagga acggcgcccc
     4921 gcccaggcga tgagtgccac cagcacggcg aagacggcga tgcccgcggc cagggccagg
     4981 gcgttgacca tcagcggggt accgtcaaag agcgcgggaa gctgggatcg ggcaaggggc
     5041 tcggacctct cgggagagac cagggcattc tagtacagcg acggaggggg agggtttcgc
     5101 gctaagatgc tggcccgagt ttcagtaatc cataccaggc cctgtgtggc cgaggagacg
     5161 gacatgaaga gagcgtacgt ttgcgggatg gctctggcgc tgctcgcgct gccgggagcc
     5221 gccgtggccc acgatcacgg cgagaagccg gcgacggcag ccggcgcggc agccggcggc
     5281 gccgaggtgc gggccgagat cctgcgccag ctcggtgacg ccgaggagaa gctgatggcc
     5341 ctcgccgagg ccatgccggc cgagaagtac ggttggcgtc cggccgaggg ggtccgcacg
     5401 gtgggggagg tcttcctcca cgtcgccggc ggcaactact tccttcccac cttttggggc
     5461 gtcaagcctc ccgaggggat cgacatgcgg gcgatcgaga aggacggtgc cgacaaggcc
     5521 aaggtgatcg ccgggctcaa gcggtccttc gaccacgcca ggaaggcgat cgcggacgtc
     5581 cccgaggccg agatgggcaa ggcgatcaag atcttcggcc gggacggctc ggtgcgggag
     5641 gcgttcatga tcgacgcgac ccatgcccac gagcacctgg gccaggccat cgcctacgcc
     5701 cggatgaacg gcatcgcccc gccctggagc cgcggcgagt agaccct
//
LOCUS       DASPJI010000184         5882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_125637, whole genome shotgun sequence.
ACCESSION   DASPJI010000184 DASPJI010000000
VERSION     DASPJI010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125637"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VEL74_04885"
     CDS             <1..282
                     /locus_tag="VEL74_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HYG61892.1"
                     /translation="VDAAVRVMESVRLCALAENLGAAETLITHPASMTHAALTPEQRR
                     AAGITDGLIRLSVGLEDPADIIDDLRRALAAGSTTKGEHPCIRRPPSIS"
     gene            252..1604
                     /locus_tag="VEL74_04890"
     CDS             252..1604
                     /locus_tag="VEL74_04890"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HYG61893.1"
                     /translation="MHPTSPIHILKLASPSLPVHEVYRAWRSGSRVVVVTPVLGPTAD
                     DLLKRAEQLGLPPDREALAALHRAGLPATLLDPSPAASVLQALANGIVVVPGGSDLTA
                     PALARELGAPCSPLPHQPARPPRSLLTVALLGCGTVGGGVLERLLARPDLFEVTGVAV
                     RDLGLSRGDGVPRHLLTDGPEALVAQGPDVVVELIGGREPARSLIVRALEQGSHVVTA
                     NKALLAEEIGRLSALAVENGVSLLYSASVGGALPALETVWRVAAGGPIRAIRGVLNGT
                     STFVLDRRAEGLSLEAAVTAAQLAGFAEANPTLDLDGSDAAQKLALLAREAFGDNLRW
                     HRIPRRGIDDVADHVLAEAARWGAVVRLVATCELLPEGLRASVRPVELDASDFLARTT
                     TGAGNALRIERVDGSVAELSAAGAGRWPTTEAVMADLHDLVDLPAAHLSAALAKEVTA
                     "
     gene            1601..2788
                     /locus_tag="VEL74_04895"
     CDS             1601..2788
                     /locus_tag="VEL74_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HYG61894.1"
                     /translation="MKIETRCVHLPAGRDPHGAMAPPIYQTAAFRQPGALEFGEYDYS
                     RTANPTRALVEEHLAALEGGRHACAFASGMAALAAVTRLVRAGEEILAGDDLYGGTCR
                     LLSQVAPGLGIRVRYVDATDPAAVEEALSPSVRLLLVETPTNPLLRIVDIARLAEIVH
                     HHGAFLAVDNSLLSPCLQRPLAHGADLVIHSATKLLCGHGDVTAGAVVTSGPELHRRL
                     AFHLNAEGAGLGPFDSWLLLRGMRTLALRTERQCASAQRVAGFLGCHPRVREVYSPLF
                     APPEARRVHERQASGPGPVVSFTTGDRELSRRIVEAARLFAIAVSFGSVASSISLPCR
                     MSHASIPPELRGRLGPPPDLVRLSVGIEDADDLTADLAQALDAAARGTSPAPLTARGG
                     DVQ"
     gene            2785..3006
                     /locus_tag="VEL74_04900"
     CDS             2785..3006
                     /locus_tag="VEL74_04900"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HYG61895.1"
                     /translation="MRHVNPQPTVVNLNTDPLAELARVQGIGRERAEALIMYREQNGP
                     LANWGELRQVPGFEDEEVVEMVRQQTILG"
     gene            complement(3007..3237)
                     /locus_tag="VEL74_04905"
     CDS             complement(3007..3237)
                     /locus_tag="VEL74_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61896.1"
                     /translation="MRFGLGRGDLLRQVEESHDPDGEELGSRRIREVLLRMAPDATPA
                     EIAQALLQASCRFTRGAEAEDDRTIVVLRVAD"
     gene            complement(3447..4718)
                     /locus_tag="VEL74_04910"
     CDS             complement(3447..4718)
                     /locus_tag="VEL74_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61897.1"
                     /translation="MSTDNEGKTTRLDRFRSWLRLLVALGLWAAFARFLAGLSPLPAP
                     TPFLTVAAVLLTLALSLVAFNSLLHFIAYMVTFPVWAPIYLIGSVVARLRVPVIGLGA
                     RLGRFYRSPLAAFLHIPVAILLEVVIYDSNKPAYLTVSLGVLLFVTFDMILITFFISP
                     SILVARRILRIARRYIEQGNMEEAVFDRKFRAWPDSAASTKARNQLEFFMTAEGWLRR
                     SGDTLLSLRSVVTSFLILLLGTFFFVAMSFGFIFLAGWKLDPAFFGCTAATPGVVDSA
                     FFSLTTITTAGGSVLCPGTVLAKSLVAIELFCGLLLLSFAGIGFSTLHATDVEGTRTE
                     FAALRREALERMVQVAKTLHLAVRLAQEKRLPDDEAARSEARSSGRTQVEVWSEWVAQ
                     AAKDLPNLPDESSAATAEFERWLAEEAGQRP"
     gene            complement(4820..>5882)
                     /locus_tag="VEL74_04915"
     CDS             complement(4820..>5882)
                     /locus_tag="VEL74_04915"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HYG61898.1"
                     /translation="RGGIPFTGVRSQSLGEGLCGAAAALRQTIRVPNVHLDPRYMGCA
                     SSLAVNSEMIVPLVLEDRLLGILDFESEKYDAFSARDEQLVSTLASSLAIALENARLY
                     ERLRLDEMRVHEDLEMARELQAQLLPKVSPWVPGLQVGFAYEPARHLGGDFYDFLSYG
                     EGRLAVAVGDVAGKSTPAALYGSFAVGMLREYATRGVYGPSQVLADMNRRLKQLSIDR
                     RFLAMMFAVYDAGARTLTLASSGLPKPFHKSGDEVREIPVHGVPLGLLSDRGYEERVI
                     QLQPGDVVVICSDGVEESHDPDGEELGSRRIREVLLRMAPDATPAEIAQTLLQASCRF
                     TRGAEAEDDRTIVVLRVAD"
BASE COUNT          911 a         2085 c         2022 g          864 t
ORIGIN      
        1 gtcgacgccg cggtgcgggt gatggagagc gtccgcctct gcgccctcgc cgagaacctt
       61 ggcgccgccg agaccctgat cacccatccc gcctcgatga cccacgccgc cctcaccccc
      121 gagcagcgcc gggcggccgg catcaccgac ggcctcatcc gtctctcggt ggggctcgaa
      181 gaccccgccg acatcatcga cgaccttcgc cgcgccctgg cggctggatc gacaacgaaa
      241 ggagaacacc catgcatccg acgtccccca tccatatcct gaagctcgcc agcccgtccc
      301 tgccggttca cgaggtctac cgcgcctggc ggtccggcag ccgtgtcgtc gtggtgaccc
      361 cggtcctggg acccaccgcg gacgacctgc tgaaaagagc cgagcagttg ggcctgccac
      421 cggatcgcga ggcgctcgcc gccctccacc gtgccggact gccggccacc ctcctcgatc
      481 cctctcctgc cgcgtccgtc cttcaagctc ttgctaacgg catcgtcgtc gtccccggcg
      541 gctcggacct caccgcgccc gccctcgccc gggagctcgg tgccccctgc tcacccctcc
      601 cgcaccagcc agcccgccct ccccgctcgc tgctcacggt cgccctcctc ggctgcggca
      661 ccgtcggcgg cggagtgctc gaacgtctcc tcgcccggcc ggacctcttc gaggtgaccg
      721 gcgtcgcggt gcgggacctc gggctctccc ggggggacgg cgtccctcgt cacctgttga
      781 cggacggccc cgaggcgctc gtcgcccagg ggcccgatgt ggtggtggag ctgatcggcg
      841 gccgcgagcc cgcccgctcg ctgatcgtcc gcgcgctgga gcaggggagc catgtggtga
      901 cggccaacaa ggcgctcctg gcggaggaga tcggacggct ctcggccctg gccgtggaga
      961 acggggtgtc gctcctctat agcgccagcg tcggcggcgc gctgccggcg ctggagaccg
     1021 tctggcgcgt cgcggccggg gggccgatcc gcgcgatccg cggtgtgctc aacggcacca
     1081 gcaccttcgt cctcgaccgg cgggcggagg ggctctcgct ggaagcggcc gtgaccgcgg
     1141 cccaactcgc cggctttgcg gaggcgaatc ccaccctcga cctggacggc tccgacgccg
     1201 cccagaagct ggcgctcctc gcccgcgagg cctttggcga caacctgcgc tggcatcgca
     1261 tcccccgccg cggcatcgac gacgtggcgg accacgtcct cgccgaggcc gcccgctggg
     1321 gcgccgtcgt ccgcctggtc gccacctgcg agctgttgcc ggaggggttg cgggcgagcg
     1381 tccggcccgt ggagctggac gcctcggatt tcctcgcgag aacgaccacc ggcgccggca
     1441 acgcgctacg cattgagcgg gtggacggct ccgtggccga gctctcggcc gccggagccg
     1501 gtcgctggcc gaccaccgag gccgtgatgg ccgacctcca cgacctcgtc gaccttccgg
     1561 cggcgcacct ctccgccgct ctggccaagg aggtgacggc atgaagatcg agacccgctg
     1621 tgtccatctg ccggccgggc gcgatcctca cggggccatg gcgccgccga tctatcagac
     1681 ggcggcgttc cggcagcccg gcgcccttga attcggcgag tacgactact cgcgcactgc
     1741 caacccgacc cgcgccctgg tggaggagca cctggcggcc ctcgaagggg gccgccacgc
     1801 ctgcgccttc gccagcggga tggctgccct cgccgcggtg acccggctgg tacgggcggg
     1861 ggaggagatc ctcgccggcg acgacctcta cggcggcacc tgccggctgc tctcccaggt
     1921 ggcgcccggg ttggggatcc gggtccgcta cgtggacgcc accgatcccg cggcggtgga
     1981 ggaggcgctg tctccatccg tgaggctcct tttggtggag actcctacca atccgctgct
     2041 ccgcatcgtg gacatcgccc gtctggcgga gatcgtccac caccacggcg cgttcctcgc
     2101 ggtcgacaac tcgctgctct ccccctgcct gcaaaggccg ctggcgcacg gcgccgatct
     2161 ggtgatccat tcggcgacca agctcctctg cggccacggc gacgtcacgg ccggagcggt
     2221 ggtgacctcc ggccccgagc tgcaccgccg gctcgccttc catctcaacg ccgaaggggc
     2281 ggggctgggc ccgttcgaca gttggctgct gctgcgtggc atgcggacgc tggcgctacg
     2341 gaccgaacgg cagtgcgcct cggcgcagcg ggtggcgggc ttcctgggct gccacccgcg
     2401 ggtccgggag gtctactccc cccttttcgc gccgccggag gcacggcggg tacacgagcg
     2461 ccaggcgtcc ggcccgggcc cggtggtgag cttcaccacc ggcgatcggg agctgtcgcg
     2521 gcgcatcgtc gaggcggcgc gcctgttcgc catcgccgtg agcttcggct cggtggcgtc
     2581 gtcgatcagc ctgccctgcc ggatgtcgca cgccagcatc cccccggagc tgcgcggccg
     2641 gctggggccg ccgccggacc tcgtccgtct ctcggtaggc atcgaggacg cggacgacct
     2701 gacggccgac ctggcccagg ccctcgacgc cgcggcccgt ggcacgtcgc ctgcacccct
     2761 gacggcgcga ggaggtgatg tccaatgaga cacgtcaatc cccagcccac cgtggtgaac
     2821 ctgaacaccg acccgctcgc cgagctggcg cgggtgcagg gcatcggccg cgagcgcgcc
     2881 gaggctctca tcatgtaccg ggagcagaac ggtcccctgg ccaactgggg cgagttgcgc
     2941 caagtgccgg gcttcgaaga cgaagaggtg gtcgagatgg tgcgtcagca gacgatcctg
     3001 ggctgatcag tccgccacgc ggaggacgac gatggtgcgg tcgtcctccg cttcggcgcc
     3061 gcgggtgaag cggcaggagg cctggaggag cgcctgggcg atctcggcgg gggtggcgtc
     3121 cggagccatc cgcaggagga cctcgcggat gcgccggctc cccagctcct cgccgtccgg
     3181 atcgtggctc tcctcgacct gtcggagcag atcaccacgt ccgaggccga agcgcacgtg
     3241 ccgtagtcca gagcgaccga gaagaagtgc tcgatcttgt cgctccggcc gcgctcgccc
     3301 cggggccgca ggtcctgccg cgccggctgt gcctccgggt cctatagcgc ggggtcgtcc
     3361 atcgcgaagg cgaccgcgag gacgatcacc caacagaacc cgcgaaagtt actggcgcgc
     3421 agccagagcc tcgaagccac acgtcatcat gggcgctggc cagcctcttc ggccagccaa
     3481 cgttcaaact cggcggtcgc tgcgctgctt tcatctggca agttcggcag gtcctttgcg
     3541 gcctgcgcga cccactccga ccacacctct acctgcgtcc tgccggagct cctggcttcg
     3601 ctcctggcgg cctcatcgtc cggcagacgc ttttcctgcg ccaatcgcac agctaaatgg
     3661 agagttttgg cgacttgaac catcctctct agagcttctc gtctcagcgc ggcgaactcg
     3721 gtccgggtcc cctcgacatc ggtcgcgtgc aaggtcgaga agccgatgcc ggcaaacgag
     3781 aggaggagca ggccacagaa gagctcgatg gccacgagcg acttggcgag caccgtgccc
     3841 gggcacagca cggagccgcc cgccgtggtg atggtcgtca ggctgaagaa cgccgagtcg
     3901 acgacgccgg gcgtcgccgc cgtacatccg aagaaggccg gatcgagctt ccagccggcc
     3961 aggaagataa acccgaagct catagcgacg aaaaagaacg tgccgaggag aaggatcagg
     4021 aaggatgtga cgactgatcg aagagagagc agcgtatcgc ccgagcgcct cagccatccc
     4081 tcggccgtca tgaagaactc caattgattc ctagccttgg tcgacgcggc ggagtccggc
     4141 caagcgcgaa acttgcgatc aaacacggct tcctccatgt tcccctgctc gatgtaccgt
     4201 cgcgcgatcc gcaagatccg ccgtgcgacg aggatggaag gggagatgaa gaacgtaatg
     4261 aggatcatgt caaaagtgac gaaaaggaga acaccaagac tgacggtcag ataagcaggc
     4321 ttgtttgaat cgtagatgac cacttcgagc aagatagcca ccggaatgtg caggaaggcg
     4381 gccaacggtg agcggtagaa tcgtccgagc cgagcgccaa ggccaatcac gggcactctg
     4441 agccgtgcca ccaccgaccc gatcaggtag atcggggccc agaccggaaa ggtgaccata
     4501 taggcgatga agtgcagcag gctattgaag gcgaccagtg agagggccag cgtcaacaga
     4561 acagcggcga cggtgaggaa gggagtcggg gcgggcagcg gcgagagccc cgcgagaaag
     4621 cgagcaaacg ccgcccacag ccccaaggcc accaggaggc gcaaccagga gcggaagcgg
     4681 tcgagtctcg tggtcttgcc ctcgttgtcc gtcgacacgt gctgatctcc tcctcggcgc
     4741 cggtctcgaa gtcaatggag gccgagcttc cggtttcatc tcgcgtggag tctctagtgc
     4801 tggccggccg tcactccgct cagtccgcca cgcggaggac gacgatggtg cggtcgtcct
     4861 ccgcttcggc gccgcgggtg aagcggcagg aggcctggag gagggtctgg gcgatctcgg
     4921 cgggggtggc gtccggagcc atccgcagga ggacctcgcg gatgcgccgg ctccccagct
     4981 cctcgccgtc cggatcgtgg ctctcctcca cgccgtcgga gcagatcacc accacgtcgc
     5041 ccggttgcaa ctggatcacc cgttcctcgt agccgcggtc ggagaggagg cccaggggga
     5101 cgccgtgcac cgggatctcc cgcacctcgt cgccgctctt gtggaacggc ttggggagcc
     5161 cggagctcgc cagggtcagc gtgcgcgccc cggcgtcgta gacggcgaac atcatggcca
     5221 ggaagcggcg gtcgatgctg agctgcttga gccggcggtt catgtccgcc agcacctgtg
     5281 acggcccgta gacgccgcgg gtggcgtact cgcgcagcat gccgacggcg aaggagccgt
     5341 agagcgccgc gggcgtcgac ttgccggcga cgtcgcccac cgccaccgcc agccgcccct
     5401 cgccatagga gaggaagtcg tagaagtcgc cgcccaggtg ccgcgccggc tcgtaggcga
     5461 agcccacctg caggccgggg acccagggcg agaccttggg caggagctgc gcctggagct
     5521 cccgcgccat ctccagatcc tcgtgcaccc gcatctcgtc cagccgcagc cgctcgtaca
     5581 ggcgggcgtt ctccagggcg atggcgagcg acgaggcgag ggtcgagacg agctgctcgt
     5641 cgcgggcgct gaaggcgtcg tacttctcgc tctcgaagtc gaggatgccc agcagccggt
     5701 cttccagcac cagcggcacg atcatctccg agttgacggc gaggctggag gcgcagccca
     5761 tgtagcgggg atcgaggtgg acgttcggga cgcggatggt ctggcggagg gccgcggcgg
     5821 cgccgcacag cccctcgccg aggctctggc tgcggacgcc ggtgaagggg atgccgccgc
     5881 gg
//
LOCUS       DASPJI010000185         3608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_814169, whole genome shotgun sequence.
ACCESSION   DASPJI010000185 DASPJI010000000
VERSION     DASPJI010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814169"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /gene="mutS"
                     /locus_tag="VEL74_04920"
     CDS             <1..1129
                     /gene="mutS"
                     /locus_tag="VEL74_04920"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HYG61899.1"
                     /translation="IRKQTLVNAERYVTPEIKELEEQILSAEERQISLEQKHFQQLTA
                     TVAAAAEGLSALAAALGTLDALAAFAEVAARHRYVRPKMLQAGAPVSIREGRHPVVEQ
                     ASRDVFVPNDVELEAERSQIVLLTGPNMGGKSTYLRQVALIVLMAQAGSFVPADQAEI
                     GVVDRIFTRVGASDDLARGESTFMVEMIETANILRHATDRSLVILDEVGRGTATFDGL
                     SLAWAIVEYLHEHRRPKTLFATHYHELTELATLLPRVVNRTMAVKEWDEKIVFLRRVV
                     PGSADKSYGLHVARLAGLPGAVIDRAGEILANLEAHEYDPSGKPRLARGNAPETGEPA
                     QLTLFVPPEQVVAAILRDAAIEELTPLAALNLLHSLKSRLI"
     gene            1126..2193
                     /gene="nagZ"
                     /locus_tag="VEL74_04925"
     CDS             1126..2193
                     /gene="nagZ"
                     /locus_tag="VEL74_04925"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HYG61900.1"
                     /translation="MRAAELVFVGIPGPELDRKTSALLAEHRPGGVVLFERNIKEIEQ
                     LHDLVRALRKVVPEAVLAVDAEGGELDRLKDMVGPAPSASLLARHAPSLSLQAGQWIG
                     RAMRLFDLDMDFAPVVDLDRGENGNALEGRYLGKTPDQILPRAQAFLRGLHTGGVGGC
                     LKHFPGLGGAGEDTHLSMSCVYLPTEELRTDLAPFQSLARLAGAVMVAHAIYPAYDGS
                     SLPATLSPRILGGLLRGRLGFDGLALSDDLEMKALASWGDLPERAQASFAAGCDVVLL
                     CHTLEALPAVVERLAGPSLADRVAEAWRRFDTYRQRLRTLRDARDYMSHMRESTRGER
                     LETIQMALEQIRESAGREGRG"
     gene            complement(2225..2530)
                     /locus_tag="VEL74_04930"
     CDS             complement(2225..2530)
                     /locus_tag="VEL74_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61901.1"
                     /translation="MPSWIDGALYPDTETPDKLETRAERVDFLARLCSAWDFGLLPDA
                     DTVDEIRRTVWREAVDAARLLTSPAYHLVRSWHGLPALPYLGQRLGYISDDPCLAHV"
     gene            complement(2514..3206)
                     /locus_tag="VEL74_04935"
     CDS             complement(2514..3206)
                     /locus_tag="VEL74_04935"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HYG61902.1"
                     /translation="MEPTPPTHLSEYAEKSLQALAREGLGHKISLGGALGLQHYHEYR
                     TTHDVDAWWHDSASAEDRARVLAVIEETLRSWGDVRARRWGEVVSLDLRVGNRTVFNF
                     QIATRSALLASPTRVPWADVALDSFPDLVASKMVALVERGAPRDFLDIYTVCHAELVT
                     ASGCWDLWRQRQRLAGSDADSRRARLAIETHLIRIAQHRPLDEIPEPEQRAEAERLRN
                     WFQKEFLDALVD"
     gene            complement(3236..>3608)
                     /locus_tag="VEL74_04940"
     CDS             complement(3236..>3608)
                     /locus_tag="VEL74_04940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HYG61903.1"
                     /translation="ARYGGEEFGLILPGVRAGRAGDLLERVRRVVAATAIHLPGHPYP
                     VSVTVSIGVASWPEDGNTALEILACADRRLYEAKARGRDRVVGPDPAALRPLDEQLRI
                     PATDSSPTGQISLAPLVPPDA"
BASE COUNT          547 a         1260 c         1280 g          521 t
ORIGIN      
        1 catccgcaag cagaccctgg tcaacgccga gcgctacgtc acccccgaga tcaaggagct
       61 ggaggagcag atcctgtccg ccgaggagcg ccagatctcc ctggagcaga agcacttcca
      121 gcaactgacg gcgaccgtcg ccgcggcggc cgaggggctc tcggccctgg ccgcggccct
      181 cggcaccctc gacgcgctgg ccgccttcgc cgaggtggcg gcgcgccacc gctacgtccg
      241 cccgaagatg ctgcaggcgg gggcgccggt cagcatccgc gaggggcgcc atccggtggt
      301 ggagcaggcg agccgcgacg tcttcgtccc caacgacgtc gagctggagg ccgagcgctc
      361 gcagatcgtc ctgctgaccg gtcccaacat gggaggcaaa tcgacctacc tgcggcaggt
      421 ggcgctcatc gtcctgatgg cccaggcggg gagcttcgtg cccgccgacc aggccgagat
      481 cggggtggtg gaccgcatct tcacccgcgt cggcgcctcg gacgacctgg cgcgcgggga
      541 gtccaccttc atggtggaga tgattgagac cgcgaacatc ctgcgccacg ccaccgaccg
      601 cagcctggtc atcctcgacg aggtggggcg gggcacggcc accttcgacg gcctgtcgct
      661 cgcctgggcg atcgtcgagt acctgcacga gcaccgccgt cccaagaccc tgttcgccac
      721 ccactaccac gagctgacgg agctggcgac gctgctgccg cgggtggtca accgcaccat
      781 ggcggtcaag gagtgggacg agaagatcgt cttcctgcgc cgggtggtgc cgggcagcgc
      841 cgacaagtcc tacggcctgc acgtggcgcg gctggcgggg ttgccgggcg cggtcatcga
      901 ccgggcgggg gagatcctgg ccaatctgga ggcccacgag tacgacccct cgggcaagcc
      961 acgcctggct aggggcaacg cgccggagac cggggagccg gcccagctca ccctcttcgt
     1021 gccgcccgag caggtggtcg ccgccatcct ccgggacgcc gcgatcgagg aattgacgcc
     1081 gctggcggcg ctgaacctcc tgcacagtct aaaatcccgg ctcatatgag agccgccgag
     1141 ctggtcttcg tagggatccc cggccccgag ctggaccgga agacctccgc gctgctcgct
     1201 gagcaccggc ccgggggggt ggtgctgttc gagcgcaaca tcaaagagat cgagcagctt
     1261 cacgacctgg tacgggcgct gcgcaaggtg gtgcccgagg cggtgctcgc cgtcgacgcc
     1321 gaaggcgggg agctcgaccg cctcaaggac atggtcggcc cggcgcccag cgcctcgctc
     1381 ctggcgcggc acgctcccag cctgtcgctc caggcggggc agtggatcgg ccgcgccatg
     1441 cgcctgttcg acctggacat ggacttcgcg ccggtggtgg acctcgaccg tggcgagaac
     1501 ggcaacgccc tggaggggcg ctatctcgga aagacccccg accagatcct gccccgcgcc
     1561 caggccttcc tccgcggcct ccacaccggg ggagtcggcg gctgcctcaa gcacttcccc
     1621 ggcctgggcg gcgccggcga ggacacccac ctctccatgt cctgcgtcta tctcccgacg
     1681 gaagagctgc ggaccgacct cgcgccgttc cagtccctgg cccggctcgc cggcgcggtg
     1741 atggtggccc atgccatcta tcccgcctat gacggatcga gcctgccggc gaccctgtcc
     1801 cccaggatcc tcggcggcct cctgcgcggg cgcctcggct tcgacggcct ggcgctctcg
     1861 gacgatctgg agatgaaggc gctcgcctcc tggggggacc tgcccgagcg cgcccaggcc
     1921 tccttcgccg ccggctgcga tgtggtcctc ctgtgccaca ccctggaggc gctgccggcg
     1981 gtggtggagc ggctggcggg accctccctg gccgaccgcg tcgccgaggc ctggcggcgc
     2041 ttcgacacct accgtcagcg cctgcgcacc ctgcgcgacg cccgcgacta catgagccac
     2101 atgcgcgaga gcacccgcgg cgagcgcctg gagaccatcc agatggccct ggagcagatc
     2161 cgcgagagcg ccggacggga ggggcggggg taggggaggc ggccggcctg gtcaggcagt
     2221 ctggtcagac atgggccagg cacgggtcgt cggagatgta gccgagtctc tgtcccagat
     2281 aggggagagc aggcaaaccg tgccaggagc ggacgaggtg atacgcaggg gaggtgagca
     2341 ggcgtgcggc gtccaccgcc tcccgccaga ccgtcctgcg gatttcgtcg acggtatcgg
     2401 cgtccggtag caggccgaaa tcccaggcgg agcagagccg ggccaggaag tcgacccgct
     2461 cggcccgcgt ctcgagctta tccggggtct cggtgtccgg ataaagagcg ccgtcaatcc
     2521 acgagggcat cgagaaactc cttctggaac cagttgcgga gcctttcggc ttccgcgcgc
     2581 tgctccggtt ccggaatctc gtccagcggc ctgtgctgtg cgatacgaat caggtgagtt
     2641 tcgatcgcga ggcgggcgcg gcgagaatcg gcgtcgctgc cggcgagccg ttggcgctgc
     2701 cgccataggt cccagcaccc ggatgcggtc accagctccg cgtgacagac agtatagatg
     2761 tccaggaagt ccctgggggc gccgcgctcc acgagcgcca ccatcttgct ggccaccaag
     2821 tcgggaaagc tgtccagcgc cacgtccgcc caagggactc gagtcgggga ggcgagcagg
     2881 gccgatcggg tcgcaatctg aaagttgaag acggtccggt tgccgaccct gaggtccagg
     2941 ctcacgacct ctccccaccg gcgggcgcga acgtctcccc agctacgaag cgtctcttcg
     3001 atcacggcga ggactcgtgc cctgtcctcg gcggaggctg aatcatgcca ccaggcgtcc
     3061 acgtcgtgag tggtccggta ctcgtggtag tgctggagcc cgagggctcc ccccagagag
     3121 atcttatggc ccagaccttc ccgggccagc gcttggagag acttctcggc gtactcggag
     3181 aggtgggtcg gaggagtggg ctccatcacg gcttctagga tagcggaggg taggtctacg
     3241 cgtccggcgg caccagcggt gccagggaga tctggccggt ggggctggag tcggtggcgg
     3301 ggatgcggag ctgctcgtcc agggggcgga gggcggcagg gtccgggccg acgacgcggt
     3361 ctctcccacg ggccttggcc tcgtagagcc gccggtcggc gcaggcgagg atctccaggg
     3421 cggtgttgcc gtcctcgggc cagctcgcca cgccgatgct caccgtcacc gagacggggt
     3481 aggggtgccc cggcaggtgg atggcggtgg cggccaccac ccggcgcacc cgctccagca
     3541 ggtcgcccgc ccgtcccgcc ctcacccccg gcaggatcag cccgaactcc tcgccgccgt
     3601 agcgcgcc
//
LOCUS       DASPJI010000186         3140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_756114, whole genome shotgun sequence.
ACCESSION   DASPJI010000186 DASPJI010000000
VERSION     DASPJI010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756114"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3140
                     /locus_tag="VEL74_04945"
     CDS             <1..>3140
                     /locus_tag="VEL74_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HYG61904.1"
                     /translation="IGMAGRFPGAANVGELWRRLRNGEECISSFSDAELAESGVDSSL
                     LADPAYVRAAGVVEGAELFDAPFFGFNPREAEIMDPQHRVFLECAWEALESAGYDPAR
                     YPGRIGVYAGVGINTYLHHIQGGGAGLLQTSAGRYQAFIANDKDFVPTRVSYKLDLKG
                     PSVNVQTACSTSLVALHLACQSLRAGDCDMALVGGVSIRSPQREGYLYEEGGILSPDG
                     HCRAFDADAKGTVFANGAGIVVVKPLEDALADGDTVLAVVKGTALNNDGSGKVGYTAP
                     SVEGQARVIAAALEAAGVEPETVSYIEAHGTGTALGDPIEVTALTEVFGSRTTRRGFC
                     ALGSIKSNLGHLDTAAGAAGLIKTVLALRHRELPPTLHFQAPNPRIDFAASPFYVNAA
                     LSEWTGGGGPLRAGVSSFGIGGTNAHVVVEEAPAPEATAPSRPWQLLALSARTPTALE
                     TATANLAANLREHPDLPLADVAYTLQMGRHAFAHRRFVVCRTEADDPAGALSDPERLV
                     TGHAETGERPVAFLFSGQGSQYPGMGQELYQIEPVFRNEIDACAELLRPHLGLDLREL
                     LYPDLFGVDERDLERTDHAQPALFAVEYALARLWMSWGVRPDAMLGHSIGEYVAACLA
                     GVFELADALALVAARGRLMQSLSQGAMLGVDLPEVEVIPLLSPDLDLAAVNAPASCVV
                     SGTREAVAALEERLAARGVRHRRLHTSHAFHSAMMRPILGAFTAEVEKVHRRPPRLPW
                     VSNLTGTWIEADEATNPAYWSRHLRSAVRFSDGVRTLLDGDAGRLLLEVGPGHTLATL
                     ARQAGPGRIVASSLPHPRDPQPDGAVLLRALGRLWASGARVDWAGFHAGERRRRVPLP
                     TYPFERQRYWLEPGTKPAAPGITDRLARRPDPADWFYVPLWQQTVPLPRLGTAEETSP
                     WLLLVENGPGASLMARLADRLNAAGCRVIAATLAPAGQGFSRLGESSWTLDPGRRENW
                     DSLLADLKAAGELPRRIVHAWNLGPADTLEASRVRAFDSLVFLAQALDRAGAGAVRIG
                     VLSSDLQRVAGERSLRP"
BASE COUNT          397 a         1200 c         1097 g          446 t
ORIGIN      
        1 atcggcatgg ccggccgctt ccccggcgcg gccaacgtcg gcgagctctg gcgccgcctg
       61 cgcaacgggg aggagtgcat ctcgagcttc tccgacgccg agctggccga gtccggggtc
      121 gattcctccc tccttgccga ccccgcctat gtccgggccg ccggggtggt cgagggggcc
      181 gagctgttcg acgcgccgtt cttcggcttc aacccgcggg aggccgagat catggacccg
      241 cagcaccggg tcttcctgga gtgcgcctgg gaggccctgg aatccgcggg ctacgacccg
      301 gcgcgctacc cgggacggat cggcgtctac gccggcgtcg gcatcaacac ctatctccac
      361 catatacaag gtggtggggc tggcctgctc cagacgtcgg ccgggcgcta ccaggccttc
      421 atcgccaacg acaaggactt cgtgccgacg cgggtctcct acaagctgga cctcaagggg
      481 cccagcgtca acgtccagac cgcctgctcg acctcgctcg tggccctcca cctggcttgc
      541 cagtcgctgc gggccggcga ctgcgacatg gcgctggtcg gcggcgtctc catccgttcc
      601 ccgcaacgcg agggctacct ctatgaggag ggggggatcc tctcccccga tggccactgc
      661 cgcgccttcg acgccgacgc gaagggcacg gtgttcgcca acggcgccgg catcgtggtg
      721 gtcaagcccc tggaggacgc cctcgccgac ggcgacacgg tgctcgccgt ggtcaagggg
      781 accgcgctca acaacgacgg ctcgggcaag gtgggctata cggcgcccag cgtcgagggg
      841 caggcgcggg tcatcgccgc cgccctggag gccgcggggg tggagccgga gacggtctcc
      901 tacatcgagg cccacggcac cggcaccgcc ctgggcgacc cgatcgaggt gacggcgctc
      961 accgaggtct tcggctcccg caccaccagg cgcggcttct gcgccctggg gtcgatcaag
     1021 agcaatctcg ggcacctgga caccgcggcc ggagcggccg ggctcatcaa gacggtcttg
     1081 gcgttgcgcc accgggagct gccacctacc ctccatttcc aggcgcccaa cccgcgcatc
     1141 gacttcgcgg cgagcccgtt ctacgtcaac gccgcgctct ccgagtggac gggcggcggc
     1201 ggaccgctcc gcgccggcgt tagctcgttc ggcatcggcg gcactaacgc ccacgtggtg
     1261 gtggaggagg cgcccgcacc cgaagcaacg gcaccgtccc gcccctggca gctcctggcg
     1321 ctttcggccc gcacccccac ggcgctggag accgccaccg ccaacctcgc cgcgaacctg
     1381 cgggagcatc cggacctccc cctggcggac gtggcctaca ccctccagat gggccggcac
     1441 gccttcgccc accgccggtt cgtggtctgc cgtaccgagg cggacgatcc ggccggcgcc
     1501 ctctcggacc ccgagcggct ggtcaccggt cacgccgaga ccggcgaacg ccccgtggcc
     1561 ttcctgttct cgggccaggg gtcgcagtac cccggcatgg gacaggagct gtaccagatc
     1621 gagccggtct tccggaacga gatcgacgcc tgcgccgagc tgctccgtcc ccacctgggg
     1681 ctggacctgc gggagctgct ctacccggac ctctttggtg tggatgagag ggacctggag
     1741 cgcaccgacc atgcccagcc cgcgctgttc gccgtcgagt acgctctggc ccggctgtgg
     1801 atgagctggg gcgtgcggcc ggacgccatg ctggggcaca gcatcggcga gtacgtagcc
     1861 gcctgcctgg ccggtgtctt cgagctggcc gacgccctcg cactggtcgc ggcccggggg
     1921 cggctgatgc agtcgctgtc gcaaggggcc atgctggggg tggacctgcc cgaggtggag
     1981 gtcatccccc tgctgagccc ggacctggac ctggcggcgg tcaacgcccc ggcctcgtgc
     2041 gtggtctccg gcacccggga ggccgtggcg gcgctggagg agcggctggc cgcccgcggc
     2101 gttcgccacc gccggctcca cacctcccat gccttccact cggccatgat gcggcccatc
     2161 ctgggcgcct tcacagccga ggtggagaag gtccaccgcc ggccaccgcg cctcccctgg
     2221 gtgtccaacc tcaccggcac ctggatcgaa gcggacgagg ccaccaaccc cgcctactgg
     2281 agccgccacc tgcggagcgc cgtgcgcttc tccgacggcg tgcggaccct gctggacggc
     2341 gacgccggcc gcctcctcct cgaggtgggg ccggggcaca cgctggcgac cctggcccgg
     2401 caggcgggcc cgggccggat cgtcgcttcg tcgctgcccc acccgcgcga cccgcaacca
     2461 gacggcgccg tgctgctgcg cgccctgggg cgcctgtggg cctccggcgc gcgggtggac
     2521 tgggcgggct tccacgccgg cgagcgccgc cgccgcgtgc cgctccccac ctatcctttc
     2581 gagcgccagc gctactggct ggagcccggg acgaagccgg cagccccggg catcaccgac
     2641 cgtctggccc gccggcccga cccggccgac tggttctacg tgccgctctg gcagcagacc
     2701 gtgcccctcc cgcgcctcgg gacggcggag gagacctctc cctggctcct cctcgtggag
     2761 aacgggccgg gcgcctccct catggcgcgt ctggcggacc ggctgaatgc tgccggctgc
     2821 cgggtgatcg ccgcgaccct cgctccggcc ggtcagggat tcagccgcct cggcgagagc
     2881 tcctggaccc tcgaccccgg ccggcgggag aactgggatt ccctcctggc cgacctgaaa
     2941 gccgcggggg agctgccgcg gcggatcgtc cacgcctgga acctcggccc ggccgacacc
     3001 ctggaagcct cccgggtgcg cgccttcgac agcctggtct tcctcgccca ggcgctcgac
     3061 cgtgccggcg ccggtgccgt gcggatcggg gtgctgtcga gcgacctcca gcgggtggcg
     3121 ggggagcggt cgctccggcc
//
LOCUS       DASPJI010000187         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_353268, whole genome shotgun sequence.
ACCESSION   DASPJI010000187 DASPJI010000000
VERSION     DASPJI010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353268"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VEL74_04950"
     CDS             <1..480
                     /locus_tag="VEL74_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61905.1"
                     /translation="SGPAYGDFAPGRPLTGSVTTRDGRRLAGRLVYDLDESETTETLD
                     APSQGVDYTIPFGLIASIVLPGGEERGAQPVRVTLHDGEELRLERAGDFGEGNAGLLI
                     FVDGRERPEYVPWTEVGQVDFARPPVMYPPLSRSEDASVLQGVRERGRGPDSMLWGP"
     gene            694..915
                     /locus_tag="VEL74_04955"
     CDS             694..915
                     /locus_tag="VEL74_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61906.1"
                     /translation="MNKKAKGRLNLSKETLRKLDDLNLKEAAGGGETLTEPSCKGTNI
                     CTNCTACATCFATCQAETGVCYASCGAAC"
     gene            1031..>2252
                     /locus_tag="VEL74_04960"
     CDS             1031..>2252
                     /locus_tag="VEL74_04960"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HYG61907.1"
                     /translation="MPSTSSTCGWQPILSAPLASRALDLAEEIARALDSAPPVRRRPA
                     ERASLAAGLPGRIVFLGYLERCRPGRGWGDRAVELLEQAMEEIAGLPVEYGLYSGFTG
                     LAWVLEHFHGWLVDEEDDPGEEIAAAVEKVLRSPLSPWEFDLISGAVGMGVYALERLP
                     RPGAGECLRGAVEHLAAAAERRDGTAVWPTPPGWVPEDRRERFPGGYVVTGLAHGVAG
                     VAAFLGEALAAGLPTRPLLDEAVRWLLAAKLPPGPGPVYPYEVAPGDPGRPTRLAWCH
                     GDPGVAAGLLVAARRAGEAAWENEALALARQAAARSAGEVSIIDAGLCHGSAGLAHIF
                     NRLYQGTGDPTLGDAARFWIDRTFDLRRPGEGVAGFLAWENDDAMRQDWRAEPGFLTG
                     AAGVGLALLAAAAPV"
BASE COUNT          288 a          785 c          847 g          332 t
ORIGIN      
        1 agcggccccg cctacggcga cttcgctccg gggcgaccgc tcacgggcag cgtcaccacc
       61 cgcgacggcc gccgcctcgc cggccggctg gtctacgacc tcgacgagag cgagaccacc
      121 gagacgctcg acgccccatc ccagggcgtg gactacacca tccccttcgg cctgatcgcc
      181 tcgatcgtgc tccccggcgg cgaggagagg ggcgcccagc ccgtcagggt gaccctccat
      241 gacggcgagg agctgcggct cgagcgcgcc ggcgacttcg gcgaggggaa cgccggcctg
      301 ctgatcttcg tcgatggccg tgagcgcccc gagtacgtgc cctggaccga ggtcgggcag
      361 gtcgacttcg cccgcccgcc ggtgatgtac ccgcccctgt ctcggagtga ggacgcttct
      421 gtcctccagg gagtacggga aagggggcgc ggcccggact ccatgctctg gggaccctag
      481 tggcattcac gttgcactaa gaatcggcgt ttcctttgtt gttcttagat ccgcctttcg
      541 ggaaagagtg atgtgcgaga aagccggcaa cccggcggtc tcccaggtgg cgtgtgggag
      601 agctcgtcat ctgacttctc cggagccttg accgtggact ctccctccac ggtgggtgac
      661 gggagatcgt ctttcacgcg aaaggagtcg gttgtgaaca agaaggccaa gggaaggctg
      721 aacctcagca aagagaccct ccgcaagctg gacgacctca acctgaagga ggcggccggc
      781 ggcggcgaga cgctcaccga gccctcctgc aagggcacca acatctgcac caactgcacg
      841 gcctgcgcga cctgcttcgc cacctgccag gccgagaccg gcgtttgcta cgcgagttgc
      901 ggcgcggcct gctgacctct aaggcgggac cgctcggccg tcgccgcggc ggtcccgcct
      961 cctgagccca accaggcccg gccgcagccg tatcgcggcc gggcctttct tcatggaggt
     1021 ctcgcgcgac atgccctcga cgtcctcgac gtgcggttgg caaccgatcc tctccgcgcc
     1081 cctggcgagc cgggcgttgg acctggccga ggagatcgcc cgcgccctcg acagcgcgcc
     1141 gccggtgcgg cggcgcccgg cggagcgggc gtcgctggcc gccggcctgc ccggcaggat
     1201 cgtcttcctg ggatacctgg agcgctgccg ccccggccgc ggctgggggg acagggctgt
     1261 ggagctgctg gagcaggcga tggaggagat cgccggcctg cccgtggagt acggcctcta
     1321 ctccggcttc accggcctcg cctgggtgct cgagcacttc cacggctggc tggtggacga
     1381 ggaggacgat cccggggagg agatcgcggc agcggtggag aaggtcttgc gttcgccgct
     1441 cagcccgtgg gagttcgacc tgatctcggg cgcggtgggc atgggcgtct acgcgctgga
     1501 gcggctgccg cgacccggtg ccggggagtg cctgcgcggc gcggtggagc atctggccgc
     1561 cgcggcggag cggcgcgacg ggaccgccgt ctggccgacg ccgccggggt gggtgccgga
     1621 ggaccggcgc gagcgctttc cggggggcta cgtcgtcacc ggtctggcgc acggcgtcgc
     1681 gggggtggcc gctttcctcg gagaggcgct ggcagcgggg ctgcccacgc gtcctctgct
     1741 ggacgaggcg gtgcgctggc tcctcgccgc gaagctgccg ccgggtcccg gcccggtcta
     1801 cccgtacgag gtggcgccgg gggatcccgg gcggccgacg cggctcgcct ggtgtcatgg
     1861 cgatccggga gtggcggccg gcctgctggt ggcggcgcgg cgcgccggcg aggcggcctg
     1921 ggagaatgag gcgctggcgc tcgcccggca ggcggcggcg cggagcgcgg gggaggtctc
     1981 catcatcgac gccggcctct gccatgggag cgccggtctc gcccatatct tcaaccggct
     2041 gtaccagggg accggagatc ccaccctggg cgacgccgcc cgcttctgga tcgaccggac
     2101 cttcgacctg cgccgtcccg gggagggggt ggccggcttc ctggcctggg agaacgacga
     2161 cgcgatgcgg caggactggc gggccgagcc cggcttcctt accggcgcgg ccggcgtcgg
     2221 cctcgccctt ctcgccgccg cggcgccggt cg
//
LOCUS       DASPJI010000188         3334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_472803, whole genome shotgun sequence.
ACCESSION   DASPJI010000188 DASPJI010000000
VERSION     DASPJI010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472803"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VEL74_04965"
     CDS             <1..926
                     /locus_tag="VEL74_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61908.1"
                     /translation="IGKLFISGFLEATLHRDPAYVRLFRDPRSARAWMPDDLYVSRFQ
                     DSTYRRLADYEEDVDVNSASRAGASIDGRGFTLWKEADLPYREGEGTKENQVAYLGWK
                     RKPGRQPAPNQAPHQAPASYALTLPPGLAGEWGLGRGSLLTVSLADTGDEAPDPKDKG
                     AKKEESEAGKKRKEQEEEREKKEKEAREEREKEEGREPLDLTVELVAANGATARLPLS
                     RFRPVPVPMPSRFTKLPEEDDLYGKAVEPILQTFELPLEAFARATPGFDPAALRTVRL
                     VFDRTPEGVVIVDDIGFAEGGERVPGAALGD"
     gene            1296..>3334
                     /locus_tag="VEL74_04970"
     CDS             1296..>3334
                     /locus_tag="VEL74_04970"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYG61909.1"
                     /translation="MGVVYRAVDLDLGREVALKFLPPQAVRPIDEARFRREAQAAAAL
                     DHPNIGTVHEIGEHNGRRFLAMAFYEGETLAERLERSGAPLPMPEVAAIAAQVASALA
                     AAHGAGIVHRDLKPSNLMLTRNGRVKLLDFGLARPMGSPTLTEEGFLVGTVAYMAPEQ
                     LSGEGSDQRADLWALGVVLYEMLTGGRPFDQGHRGLVHAILHEDPPPLRRGRPEIPQA
                     LAVIVERCLEKDPSRRPSGGEILTELRAAGLVSGEVVSSGATTPSVPARPRALRRWGA
                     ALAAALLLLGGVAAFQLTQRPEPLASELVVAVLAPTVSGTATATQRELVATNLEATAL
                     RTLYAVKGLTPLEPQQGEAIEGSATTIAKVTAAQEVLSTRADCTPDLCRVDLRRLAGG
                     DGRVLWAKTLQISTSGLRAAAEALAASFQQAYPSHALQSFSLELNINEADYSRYLALH
                     QRLTTTRPENLTGELMDEIELLRVRSSRFLDTYHLESKVAFWLFHETREARYLEQGLR
                     VAREARELAPDDPRPLDSLISLYLMAQRLDEAEAELAHLEILEPEWPELFPLKSSLAR
                     RRGDMDESRAYMRQAVERYPSVSNLIAAGNVEFQLGRFDDARRYFEALLARVPDHSKG
                     LRALALSELLLGGDPERAVGALRRLVLKSPGAPGPLNELGSAFMILRRYAEAETTL"
BASE COUNT          533 a         1125 c         1146 g          530 t
ORIGIN      
        1 agatcggcaa gctcttcatc tcgggcttcc tggaggcgac gctgcaccgc gacccggcct
       61 acgtgcggct cttccgggac ccgcgcagcg cccgcgcctg gatgccggac gacctctacg
      121 tctcccgctt ccaggactcg acctatcgcc ggctggccga ctatgaagag gacgtggacg
      181 tgaacagcgc cagccgcgcc ggcgcgtcca tcgacggccg cggcttcacc ttgtggaagg
      241 aggcggacct gccgtaccgc gaaggcgagg gcaccaagga gaaccaggtg gcctacctgg
      301 gctggaagcg caagccgggc cggcagccgg ccccgaatca ggccccccat caggcaccgg
      361 cgtcctacgc gctcaccctg ccgccgggcc tcgccgggga gtggggcctg ggccgcggct
      421 cgctcctcac cgtctccctc gccgacaccg gcgacgaggc gcccgatccg aaggacaagg
      481 gggcgaagaa ggaggagtcc gaggccggga agaagaggaa ggagcaggag gaggagcgcg
      541 agaagaagga gaaggaggcg cgcgaggagc gggagaagga agagggcagg gagcccctgg
      601 acctcaccgt cgagctggtc gcggccaacg gcgccaccgc ccggctcccc ctgtcgcggt
      661 tccggcccgt gccggtgccg atgccgtccc gcttcaccaa gctccccgag gaggacgacc
      721 tctacgggaa agccgtggag ccgatcctcc agaccttcga gctccccctg gaggccttcg
      781 cccgcgccac ccccggcttc gacccggcgg ccctgcgcac cgtccgcctg gtcttcgacc
      841 gcacccccga gggggtggtc atcgtggacg acatcggctt cgccgagggc ggggagcggg
      901 tgccgggggc ggcgctcgga gactgagtcg gcgagacttt tggcgagtag tcggttaata
      961 tctgcattca atcgaagaca ggtgccgtag ttcaagggca gccatgggct ctacgaggag
     1021 cttcctaaag aagctcctgg cccggcattg ggacggcggc agcctgacgg aggcgccaga
     1081 tgaccggcat tctctgcacc gcgtgcggcg cggccaactc agagtccacc cccctctgtg
     1141 ccacctgtgg cgagatcctg cccaccgtct cccccggcga ctcgacgcgg gtggatggag
     1201 cggcgtggca ggggagcgcc gcaccagccg cagatccgct ggtagggagc cgcgtctccc
     1261 atttccgggt catcggactg gtcgggcggg gaggcatggg tgtggtctac cgcgcggtgg
     1321 acctcgacct ggggcgggag gtagcgctca agttcctgcc ccctcaggcg gtccgcccga
     1381 tcgatgaggc ccgcttccgg cgcgaggccc aggccgcagc agctttggac catcccaaca
     1441 tcggcacggt ccacgagatc ggcgagcaca acggccggcg tttccttgcc atggcgttct
     1501 acgaaggaga gaccctggcg gagcggctgg agcggtcggg cgcgccctta ccgatgccgg
     1561 aggtggcggc gattgccgcc caagtcgcct cggccctggc cgcggcgcac ggcgccggca
     1621 tcgtccaccg cgacctcaag ccgtcgaacc tgatgctcac ccggaacgga cgggtcaagc
     1681 ttctcgactt cggtctcgcc cggccgatgg gctcgccgac gctcaccgag gaagggttcc
     1741 tggtcggcac cgtcgcctac atggcgccgg agcaactcag tggagaaggg agtgaccagc
     1801 gcgccgacct ctgggcactg ggtgtcgttc tctacgagat gctcaccggc ggccggccct
     1861 tcgaccaagg ccacaggggc ctggtgcacg ccatcctgca cgaggacccg ccgccgttgc
     1921 ggcgggggcg gccggagatc cctcaggcgc tcgcggtcat cgtcgagcgc tgtctggaga
     1981 aggaccccag ccgccgcccg tccggtggcg agatcctgac cgagctgagg gcggccgggc
     2041 tggtctcggg cgaggtcgtc tccagcggtg cgacgacacc cagcgtgccg gctcggcccc
     2101 gggctctccg gcgatggggc gcggcgctgg cggcagcact ccttctcctt ggtggtgtgg
     2161 ctgccttcca gctgactcag cggccggagc cgctagcgtc ggagctcgtc gtggcggtgc
     2221 tcgcgccgac ggtcagtggc acggcgaccg ctacacaacg cgagctcgtc gccaccaatc
     2281 tcgaagctac ggcactccgg acgctctacg ctgttaaggg attgacccct cttgagccac
     2341 agcaggggga agctatcgag ggatcggcca cgaccatcgc caaggtcaca gcggctcagg
     2401 aggttctctc cacacgtgct gactgcaccc ccgacctttg ccgtgtcgac ctgcgccgac
     2461 tggccggggg cgacggccgg gtgctttggg ccaagacgct gcagatctcc acctccggtt
     2521 tgcgggccgc tgcggaagcc ctggcggcga gttttcagca ggcctatccc agccatgcgc
     2581 tgcagtcttt cagtctcgag ttgaacatca atgaggcaga ctacagccgg tatctcgctc
     2641 tgcatcagcg gctcactacg acccggcccg agaatctgac cggcgagcta atggacgaaa
     2701 tcgagttgct ccgcgtccgc tcttctcgtt tcctggatac ctatcatctg gaatctaagg
     2761 tcgccttctg gctgttccat gagacccgcg aggcgcgcta tctagagcag ggtctgcgag
     2821 tggcaagaga ggcgcgggag ctggcgccgg acgaccctcg tcccctcgat agccttatct
     2881 ctctctactt gatggcccag cgtctcgacg aggctgaggc ggagttggct caccttgaga
     2941 tcctggagcc cgagtggccc gagctgttcc cgctcaagtc gagtctcgcg aggcgccggg
     3001 gggacatgga cgaatctcgg gcgtacatgc ggcaggcggt ggagcgctat ccctcagtgt
     3061 ccaacctgat tgcagccggc aacgttgagt ttcaactcgg ccggttcgat gacgcccgcc
     3121 gttattttga ggcgctgctg gcgcgcgttc ccgatcacag caagggatta agggcactgg
     3181 cgttgagtga gttgctcctg ggaggcgatc ccgagcgggc agttggagcg ctgcggcgtc
     3241 tggtactgaa atctcccggc gcacccggtc cccttaacga gcttggcagc gcgttcatga
     3301 ttctgcgccg ctatgccgaa gccgagacca cgct
//
LOCUS       DASPJI010000189         3990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_10933, whole genome shotgun sequence.
ACCESSION   DASPJI010000189 DASPJI010000000
VERSION     DASPJI010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10933"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VEL74_04975"
     CDS             complement(<1..417)
                     /locus_tag="VEL74_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010592905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61910.1"
                     /translation="MAYTAEKPRIARSSEELRRTIPGWGVDLDPKDRPATPKEKFNPE
                     GTGAHWHFPERQPEHWPRERTPEHKFLTPVFGTVCPPKGLSGAIRRYAYRYSEGRLTH
                     WLLLVGADRVDVLESRVTGLLRGRPDNPFTEAGLGSE"
     gene            complement(436..1584)
                     /locus_tag="VEL74_04980"
     CDS             complement(436..1584)
                     /locus_tag="VEL74_04980"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HYG61911.1"
                     /translation="MHAMVYRGPYRVRVEKKDIPAIEHPNDAIVRVTCSAICGSDIHL
                     YHGLMPDTRVGMTFGHEFIGVVEEVGPSVQNLQVGDRVMVPFNIFCGSCFFCSRELYA
                     NCHNVNANASAVGGIYGYSHTAGGYDGGQAEYVRVPFADVGPSRIPDWMDEEDAVMLT
                     DACPTGYFGAELGEIKEGDVVVVFGAGPVGLFAAKSAWLMGAGRVIVVDHLDYRLEFA
                     REFAHAETINFTQVKDIVMLIKKETDFLGADVCIDAVGAEADGSFFQMVTATKLKLQG
                     GSPVALNWAIDCVRKAGNVSVVGGYGPLFSAVKFGDAMNKGLTIRTNQCSVKRYWPRL
                     FEHVKNGVLKPSDIVTHRLPLEEIAEGYHMMASKLDGCIKPLVYPRTH"
     gene            complement(1721..1797)
                     /locus_tag="VEL74_04985"
     tRNA            complement(1721..1797)
                     /locus_tag="VEL74_04985"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1761..1763),aa:Arg,seq:acg)
     gene            complement(1886..3244)
                     /locus_tag="VEL74_04990"
     CDS             complement(1886..3244)
                     /locus_tag="VEL74_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61912.1"
                     /translation="MTGIHLTRDLLWAASRGELPPGAIAQMGLEHLMSLCPTCRQEIE
                     RFQKEQTAAAGATGSSQVFQTVPPLPEEQPIPIEKEERQAERDLEDLLGRPHEERVLR
                     VERSWVRFRSATLVRLLIAESRKRVQAEPGEALRLASLARLIAHRNPRIPNSFGLLAL
                     ATAEMANACRVGDDPRQADEHFQHARYVITHLGVTDTAILGRIDHLEGSLRMDQRRFK
                     RAQGLLTRAAMLYRVSGDTVETARVLITLGLMLFHRGDTALAAEATTAAIEGLRDGQE
                     PRLLMNARYNLALFLTEDGKYDAAAAMLDADADLYRAFPEPWTQLRLHWLRGKITLGQ
                     GDEVDAEWIFLEVRDGFIAQGNGYDAAMVSIEDLAQLYARKGRTADLRRLAEEIYPIF
                     QAQDVHREAMAALLLFQEAARQEALTVQAVREYATYFRDARADPSLRFGQGSPGTGEP
                     PR"
     gene            complement(3371..3667)
                     /locus_tag="VEL74_04995"
     CDS             complement(3371..3667)
                     /locus_tag="VEL74_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61913.1"
                     /translation="MNHTLQSRVRVLLLTLLLASSPARPAASAIPAGVLDLPSASSIL
                     SSLWDLLTGEKSTTAAPNGEPQPAATPSGSGTGGSGTSSGDGDGDRGSGMDPNG"
     gene            complement(3791..>3990)
                     /locus_tag="VEL74_05000"
     CDS             complement(3791..>3990)
                     /locus_tag="VEL74_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61914.1"
                     /translation="LEPGHPDLAKAQLGLGFCLFELGRDQEAEALLREGRATLAAQAG
                     PRDELVRQADEQLAALAARRR"
BASE COUNT          622 a         1331 c         1401 g          636 t
ORIGIN      
        1 ctcgctcccg agccccgcct cggtgaacgg gttgtcaggc cggccgcgca gcaggccggt
       61 cacccggctc tccagcacgt ccacccggtc ggcgccaacg agaagcagcc agtgcgtcag
      121 ccgcccctcg ctgtaccggt aggcgtagcg gcggatcgcg ccggacagcc ccttgggagg
      181 gcagacggtg ccgaagaccg gcgtcaggaa cttgtgctcg ggagtccgct cccggggcca
      241 gtgctccggc tgccgctcgg ggaaatgcca gtgggcgccg gtcccctcgg ggttgaactt
      301 ctccttgggg gtggcggggc ggtccttggg atcgaggtcg accccccagc cagggatggt
      361 gcggcggagc tcctcgctcg aacgggcgat tctgggcttc tctgcagtgt aggccatgac
      421 gatctccctt ccttgctagt gcgtccgcgg atagaccagg ggcttgatgc acccgtcgag
      481 cttgctggcc atcatgtggt agccctcggc gatctcctcg agcgggaggc ggtgggtgac
      541 gatgtcgctc ggcttgagca cgccgttctt cacgtgctcg aacaggcgcg gccagtaccg
      601 cttgacgctg cactggttgg tgcggatggt cagccccttg ttcatcgcgt cgccgaactt
      661 cacggcgctg aacagcgggc cgtagccccc cacgacggag acgttgccgg ccttccggac
      721 gcagtcgatg gcccagttga gcgccaccgg cgagcccccc tgcagcttga gcttggtcgc
      781 ggtcaccatc tggaagaagc tgccgtccgc ctccgcgccg acggcgtcga tgcagacgtc
      841 ggcgccgagg aagtcggtct ccttcttgat gagcatcacg atgtccttga cctgggtaaa
      901 gttgatcgtc tcggcgtggg cgaactcgcg ggcgaactcc agccgataat cgaggtggtc
      961 gaccacgatg acgcgcccgg cgcccatcag ccaggccgac ttggcggcga acagcccgac
     1021 cggcccggcg ccgaagacca ccaccacgtc cccctccttg atctcgccga gctcggcgcc
     1081 gaagtagccg gtggggcagg cgtcggtgag catcaccgcg tcctcctcgt ccatccagtc
     1141 agggatccgg ctcggcccga catcggcgaa cggcacgcgc acgtactccg cctggccgcc
     1201 gtcatagccg cccgcggtgt gcgagtagcc gtagatgcca ccgacggcgc tggcgttggc
     1261 gttgacgttg tggcagttgg catagagctc ccgcgagcag aagaagcagg agccgcagaa
     1321 gatgttgaac ggcaccatga cccggtcgcc gacctgcagg ttctgcaccg acgggccgac
     1381 ctcctcgacc acgccgatga actcgtggcc gaacgtcatc ccgacgcggg tgtcgggcat
     1441 cagcccatga tagaggtgga tgtccgagcc gcagatcgcg gagcaggtga cccgcacgat
     1501 ggcatcgttc gggtgctcga tcgcggggat gtccttcttc tcgacacgga cccggtaggg
     1561 tccgcggtag accatcgcgt gcatcatgtt ggggtctcct ctcaggtgtt cattccgacg
     1621 cactcgtcag gaggaggagt tgctgcaagg ccactgccag cgcgcggcga gtcgcgtcgc
     1681 gatgaaacga actgagagct ggtcagggaa gaagtagaag tggtgcagcc aggaggagtc
     1741 gaacccccaa cctacagatc cgtagtctgt cgctctatcc agttgagcta tggctgcacg
     1801 atgtgtccaa ctcgtgcgga ccccgaattg tagggcaggc cgggacgtca tgcaaccccc
     1861 cggattcatc cggattcaac cggcttcacc gggggggctc acccgttcca gggctcccct
     1921 gcccgaagcg gagggacggg tcggcgcggg cgtcccggaa gtaggtcgcg tactcgcgga
     1981 cggcctgcac ggtgagcgcc tcctgccggg cggcctcctg gaagagcagc agcgcggcca
     2041 tcgcctcgcg gtggacgtcc tgggcctgga agatcgggta gatctcctcg gcgagccgcc
     2101 ggaggtccgc cgtccgtccc ttccgcgcgt agagctgggc gaggtcctcg atcgagacca
     2161 tggcggcgtc gtagccgttg ccttgggcga tgaagccgtc ccgcacctcc aggaagatcc
     2221 actcggcatc cacctcgtca ccctggccga gggtgatctt gccgcgcagc cagtggagcc
     2281 ggagctgggt ccagggctcc gggaacgcgc ggtagaggtc cgcgtcggcg tcgagcatcg
     2341 ccgccgcggc gtcatacttc ccgtcctcgg tgaggaagag cgccagattg tagcgggcgt
     2401 tcatcagcag gcgcggctcc tggccgtccc gcagcccctc gatcgccgcc gtcgtcgcct
     2461 cggcggcgag cgcggtgtcc ccccggtgga agagcatcaa tcccagagtg atcagcaccc
     2521 gggcggtctc caccgtgtcg ccgctcaccc ggtagagcat cgcggcgcgg gtgaggaggc
     2581 cctgggccct cttgaagcgc cgctggtcca tgcgcaggga gccctccagg tggtcgatgc
     2641 ggccgaggat cgccgtatcg gtgacgccga ggtgtgtgat gacgtaccgg gcgtgctgga
     2701 agtgctcgtc cgcctgccgg ggatcgtccc ccacgcggca ggcattggcc atctcggccg
     2761 tcgccagggc gaggagcccg aaggagttcg gaatccgggg attccggtgg gcgatgagcc
     2821 gggccaggga cgccaagcgg agcgcctcac ccggctcggc ctgtacccgc ttgcggctct
     2881 ccgcgatgag gagacgcacc aacgtggcgc tacggaaccg cacccaggag cgctccaccc
     2941 gcagaaccct ctcctcgtga ggccggccga ggagatcctc caggtctcgc tccgcctgcc
     3001 gttcctcctt ctcaatagga ataggctgct cctcggggag aggcgggacg gtctgaaaca
     3061 cctggctgga gccggtggct ccggccgccg ccgtctgctc cttctggaac ctctcgatct
     3121 cctgccggca ggtagggcag aggctcatca ggtgctccag ccccatctgc gcgatggcgc
     3181 ccgggggcag ctcgccacgg gaggcagccc agaggaggtc gcgggtgaga tggataccgg
     3241 tcatgaattc tcctcgtgcg gaacaaatcc ttgctcagtc gcccaggcca gtataccgca
     3301 agaaagagcc accatctgga agaaagatct tgtctgggga acacttcgag cccccacgcg
     3361 atggaaaggc ctatccgttc gggtccatgc ccgatccccg gtcgccgtcg ccgtcgccag
     3421 aactggtgcc cgagccgccc gttcccgagc cggacggcgt cgcggcgggt tggggctcgc
     3481 cgttcggagc cgccgtggtg gacttctcgc cggtgaggag atcccagaga gacgagagga
     3541 tggaggaggc gctcgggagg tcgaggacgc cggccggtat cgccgatgcc gcggggcgag
     3601 ccggcgacga ggccaggagc agggtcagga ggaggacgcg gacgcgagac tgcaaggtgt
     3661 ggttcaagga acccccttct tcgcggcggc tccgcgcgga ccccgggatg gggcccgggg
     3721 ccgctcttga tataggtgct caggcgtagg taaggcgagt cttcggggag aacggaggca
     3781 gacgccaggc tcagcggcgc cgggccgcga gcgccgcgag ctgttcgtcc gcctggcgca
     3841 ccagctcgtc ccgcggtccg gcctgcgccg cgagggtggc gcgcccctcg cgcagcaggg
     3901 cctcggcctc ctggtcgcgg ccgagctcga agaggcagaa gccgagcccg agctgcgcct
     3961 tggccaggtc cggatgcccg ggctccagcg
//
LOCUS       DASPJI010000190         5004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_814770, whole genome shotgun sequence.
ACCESSION   DASPJI010000190 DASPJI010000000
VERSION     DASPJI010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814770"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VEL74_05005"
     CDS             <1..661
                     /locus_tag="VEL74_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYG61915.1"
                     /translation="QLYDRRPFFLAPDLSPVFLSNAGLDPQRALSWEAGARSPLGTAA
                     WTEIALYRMEVGDEIGFDLARLRFANIDRSLHQGIEWGLAAEAGPLRPRLAYTLTEAT
                     FEGGAHDGRRINGIPRHQIAAGLGWEARALTGEVEARHVRGQFADEDNRFPLDPYTLL
                     NAAVDWKLSRFSLGLTVRNLLGADFETSGFVTLDETGAPLPLYYPGAERAVVGRLSLE
                     L"
     gene            791..1558
                     /locus_tag="VEL74_05010"
     CDS             791..1558
                     /locus_tag="VEL74_05010"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYG61916.1"
                     /translation="MRDIATLSPSLQGTGRFTEDSPDSSSHSSGNRLASVVGNTVLAV
                     SGAVLVGYALKQRSWKAAGVALAGAPLAYRQATGSWPLQRTEEALPVTVETSVTIMKP
                     REELYSFWRRLENLPRFMRHLDSVTESGDGISHWVGRSPLGMKVEWDAEVIEEREGHL
                     LSWRSLPGSQVHNAGSVFFEDAPANRGTIVRVVFELQPPAGPIGRAVGKAMSPMTEQQ
                     VKQDLRRFKNLMEAGEIPTTDGQPAGKRPAINIHNPF"
     gene            1598..2872
                     /locus_tag="VEL74_05015"
     CDS             1598..2872
                     /locus_tag="VEL74_05015"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HYG61917.1"
                     /translation="MRAVCWNGVNEVRVETVPDPEILNPRDVIVRVTTAAICGSDLHL
                     YDGYIPAMKKGDILGHEFMGEVVELGTDVKNVQVGDRVVVPFNISCGQCFFCQKEMFS
                     LCDNTNPNSVMAEKMFGYSPAGLYGYSHLTGGYAGGQADYVRVPFADVNVLKVPEGME
                     DEKALFLGDIFPTGYMAAENCDIEPGDTVAVWGCGPVGQFAIQSAWMLGAERVIAIDR
                     FPERLLLAEGWGRAEILDYEQVDSVLDTLRDMTGGFGPDCCIDAVGMEAHGSGLDGFY
                     DKAKQSLRLESDRPTVLRDVIMACRKGGTVSIPGVYGGFGDKIPIGAMMNKGLTIKTG
                     QTHVQRYMRPLLDRIQAGEIDPSRIISHSLDLEDAPEGYETFKHKEDNCIKVILKPHA
                     HGNGHSHHHGGNGNGRHRHGMEGERDHTGKGL"
     gene            2872..4005
                     /locus_tag="VEL74_05020"
     CDS             2872..4005
                     /locus_tag="VEL74_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG61918.1"
                     /translation="MQGAASREGGQRRRPSSKVLLAAAAGFGLAAAARALRRRQPMDF
                     RDKVVLITGGSRGLGLLIGRELAARGARLAILARDPAELDLARRELAALGGQRVFAVP
                     CDVGNRDEARWAVDTVAERFGRLDVLINNAGIIQVGPLEHMQLEDFEEAMRVHFWGPL
                     YLTWAALPHLRHQEGGRIVNISSIGGKVAIPHLVPYSASKFALAGLSDGLRAELAKEG
                     IYVTTVCPGLMRTGSHVNAMFKGKREEEFSWFAVADANPLASISGRRAARRIVEACRY
                     GEPHLTLTLQAKLAVAASHVAPCLTGRALEAVARLLPGPAEQDGDEARPGWRYSSRWA
                     PSLLTRLADEAAVENNELRRQAVEYGKYGNGKSRPALGEGPGA"
     gene            complement(4030..>5004)
                     /locus_tag="VEL74_05025"
     CDS             complement(4030..>5004)
                     /locus_tag="VEL74_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYG61919.1"
                     /translation="TIDTWSMDNGTIPEEVFLEDVKLTVDKDREMLRGFLERKDEWDV
                     LVHYFEFTDRVQHMMFRFFDPKHPLNTPEGSAKWGGSILQSYQEMDAIVGEVMQKMPK
                     DTILMVVSDHGFASFRRGMNYNTWLAKNGFMKLTGEDPERKNLEDLFDQGDFFKNVDW
                     SQTRAYALGLGQIYINLKGRESKGIVEPGEEYKRVAAEIKAGLEAFVDPLTGERPVAH
                     VFTRDEAYGTFDPELIPDLIPSNSEGYRVGWQDSLGGIAKEIVEDNTNIWSGDHCSVY
                     PPLVEGIFFSSIPMDARDAYMGDVMPTILDLYGVQPTAKLDGRSFVKK"
BASE COUNT          775 a         1785 c         1646 g          798 t
ORIGIN      
        1 gcagctctat gaccggcgcc ccttcttcct ggcgccggac ctgtcccccg tcttcctgtc
       61 caacgccggc ctcgacccgc agcgggcgct gagctgggag gccggggcgc gctcccccct
      121 gggaaccgcc gcctggactg aaatcgccct ctaccggatg gaggtcggcg acgagatcgg
      181 cttcgacctc gcccggctcc gcttcgccaa catcgaccgc agcctgcacc aggggatcga
      241 gtggggcctc gcggccgagg ccggaccgct ccgcccgcgc ctcgcctaca cgctgaccga
      301 ggccaccttc gagggcggcg cccacgacgg ccggcggatc aacggcatcc cccggcacca
      361 gatcgccgcc ggtctcggct gggaggcgcg ggctctcacc ggtgaggtcg aggcgcgtca
      421 cgtccgcggc cagttcgccg acgaggacaa ccgcttcccc ctggatccct acactctact
      481 gaacgccgca gtggactgga agctctcccg cttctccctg gggctcaccg tgcgcaacct
      541 cctgggggcc gatttcgaga ccagcggctt cgtcaccctg gacgagacgg gggcaccgct
      601 ccccctctac tacccagggg ccgagcgggc cgtcgtgggg cggttgagcc tggagctctg
      661 agctccgtgg cacgtccctt gcgacccttt ccgtctgagc cggccgccgt ggctcggcgc
      721 agtcctcacc acaggagggg cccgcccgtc catagtcacc ctttcttaca tttccaggag
      781 gtacgacacc gtgcgagaca tcgccacttt gagtccttca cttcagggca cggggcgctt
      841 caccgaagat tcccccgact cttcttccca ttcctcgggc aaccggctcg cgtcggtcgt
      901 cggcaatacg gtcctcgccg tgagcggcgc ggtgctcgtg ggctatgccc tcaagcagcg
      961 ctcgtggaag gccgccggcg tcgccctggc gggcgctccg ctcgcctatc gccaagccac
     1021 cggctcctgg ccgctccagc gcaccgagga ggccctgccc gtcaccgtcg agaccagcgt
     1081 caccatcatg aagccgcgcg aggagctcta ctccttctgg cgccggctgg agaacctccc
     1141 ccggttcatg cggcacctcg actccgtcac cgagagcggc gacgggatct cccactgggt
     1201 cggccgcagc cccctcggga tgaaggtcga atgggacgcc gaggtcatcg aggagcgcga
     1261 ggggcacctc ctctcctggc gctcgctccc tggctcgcag gtccacaacg cgggctcggt
     1321 cttcttcgag gacgctccgg cgaaccgcgg caccatcgtg cgcgtcgtct tcgagctcca
     1381 gccgccggcc ggccccatcg gccgcgccgt gggcaaggcg atgagcccga tgaccgagca
     1441 gcaggtcaag caggacctcc ggcgcttcaa gaacctgatg gaggccggcg agatcccgac
     1501 caccgacgga cagcccgcgg gcaagcgccc cgcgatcaac atccacaacc ccttctgagg
     1561 atcatcagcg ccctcgcgag gggccgggag tgcaaccatg agagcagtct gttggaacgg
     1621 cgtcaacgaa gtccgggtcg agaccgtccc cgatcccgag atcctcaatc cccgggacgt
     1681 catcgtccgc gtcaccaccg cggcgatctg cggctccgac ctccacctct acgatggcta
     1741 catccccgcc atgaagaagg gggacatcct cggccacgag ttcatgggcg aggtcgtcga
     1801 gttggggacc gacgtgaaga acgtccaggt gggcgaccgc gtcgtcgtcc ccttcaacat
     1861 ctcctgcggc cagtgcttct tctgccagaa ggagatgttc tcgctgtgcg acaacacgaa
     1921 ccccaactcc gtcatggcgg agaagatgtt cggctactcg ccggccggcc tctatggcta
     1981 ttcccacctg accggcggct acgccggagg ccaggccgac tatgtgcggg tccccttcgc
     2041 cgacgtcaac gtcctcaagg tccccgaggg catggaggac gagaaggccc tgtttctggg
     2101 ggacatcttc cccaccggct acatggcggc ggagaactgt gacatcgagc ccggcgacac
     2161 cgtcgccgtc tggggctgcg gaccggtcgg ccagttcgcc atccagagcg cctggatgct
     2221 cggcgccgag cgggtcatcg ccatcgaccg cttccccgag cggctgctcc tcgccgaagg
     2281 ttggggcagg gccgagatcc tcgactacga gcaggtggac agcgtcctcg acacgctgcg
     2341 cgacatgacc ggcggcttcg gtcccgattg ctgcatcgac gcggtgggca tggaggctca
     2401 cggctcgggc ctggacggct tctatgacaa ggccaagcag agcctgcgcc tcgaatccga
     2461 ccggccgacc gtcctgcgcg acgtcataat ggcctgccgc aaggggggca cggtctccat
     2521 ccccggggtc tatggcggct tcggcgacaa gatccccatc ggcgccatga tgaacaaggg
     2581 gctgaccatc aagaccggcc agacccacgt ccagcgctac atgcgtcccc tcctcgaccg
     2641 catccaggcc ggggagatcg acccctcccg gatcatcagc cacagcctgg acctggagga
     2701 cgcgccggag ggctacgaga ccttcaagca caaggaggac aactgcatca aggtcatcct
     2761 caagccccac gcccatggca acggccactc gcaccaccac ggcggcaacg gcaacggacg
     2821 ccaccgccac gggatggaag gcgagcgcga ccataccggc aaggggttgt gatgcagggc
     2881 gctgcctccc gggagggtgg acagcgccgc cgcccgtctt ccaaggtcct cctcgcggcg
     2941 gcggccggct ttgggctggc cgccgccgcg cgggccctgc gccggcggca gcccatggac
     3001 ttccgtgaca aggtcgtgct gatcacgggc gggtcacggg gtctggggct cctcatcggc
     3061 cgtgagctcg cggcccgggg ggcgcggctg gcgatcctgg cgcgggaccc ggcggagctc
     3121 gacctcgccc ggcgcgagtt ggcggccctg ggggggcagc gggtcttcgc cgtcccctgt
     3181 gacgtcggga accgcgacga ggcgcgctgg gcggtggata cggtcgccga gcgcttcggg
     3241 cggctggacg tgctgatcaa caacgccggc atcatccagg tgggccccct ggagcatatg
     3301 cagctcgagg acttcgagga ggccatgcgg gtccacttct ggggcccgct ctacctcacc
     3361 tgggcggcgc tcccccacct gcgccaccag gagggcgggc ggatcgtcaa tatctcgtcc
     3421 atcggcggca aggtggcgat cccccacctc gtcccctact cggccagcaa gttcgccctc
     3481 gccggcctgt ccgacgggct gcgcgcggaa ctggccaagg aggggatcta cgtcacgacc
     3541 gtctgcccgg ggctgatgcg cacgggctcg cacgtcaacg ccatgttcaa ggggaagcgg
     3601 gaagaggagt tctcgtggtt cgcggtcgcc gacgccaatc cgttagcctc catcagcggc
     3661 cggcgggcgg cgcggcggat cgtcgaggcc tgccgctacg gcgagccgca cctgaccctc
     3721 accctccagg ccaagctcgc tgtggcggcg tcccatgtgg cgccgtgcct gaccggccgg
     3781 gctctggaag cggtggcacg cctcctcccc ggccccgccg agcaggacgg cgacgaggcc
     3841 aggccgggct ggaggtactc ctcccgctgg gccccctcgc tcctcacccg tcttgccgac
     3901 gaggcggcgg tggagaacaa cgagctgcgg cggcaggcgg tggagtatgg gaagtacggg
     3961 aacgggaaga gccgcccggc gctcggcgag ggcccagggg cttgagcccc tgggcctgcg
     4021 ccctgtctcc tacttcttga cgaagctccg gccgtcgagc ttcgccgtcg gctggacgcc
     4081 gtagaggtcg aggatggtgg gcatgacgtc gcccatgtag gcgtcccggg cgtccatcgg
     4141 gatgctactg aagaagatgc cctccaccag cggcgggtag accgagcagt ggtcgccgct
     4201 ccagatgttg gtgttgtcct cgacgatctc cttggcgatg ccgcccaggc tgtcctgcca
     4261 gccgacgcga tagccctcgc tgttcgacgg gatcaggtcc gggatcagct ccgggtcgaa
     4321 ggtgccgtag gcctcgtcgc gggtgaagac atgggccacc ggacgctcgc cggtcagcgg
     4381 atcgacgaag gcctcgagcc cggccttgat ctccgcggcc acccgcttgt actcctcgcc
     4441 cggctcgacg atccccttgg actcgcgccc cttgaggttg atgtagatct ggcccaggcc
     4501 cagcgcgtag gccctggtct gcgaccagtc gacgttcttg aagaagtcgc cctggtcgaa
     4561 gaggtcctcc aggttcttgc gctcgggatc ctcgccggtc agcttcatga agccgttctt
     4621 agccagccag gtgttgtagt tcatgccgcg ccggaaggag gcgaagccgt ggtccgaaac
     4681 caccatcagg atggtgtcct tcggcatctt ctgcatcacc tcgccgacga tggcgtccat
     4741 ctcctggtaa gactggagga tcgagccccc ccacttcgcc gacccctcgg gggtgttcag
     4801 cggatgcttg gggtcgaaga accggaacat catgtgctgg acgcggtcgg tgaactcgaa
     4861 atagtgcacc agcacgtccc actcgtcctt gcgctccagg aagccgcgca gcatctcgcg
     4921 gtccttgtcg accgtcagct tgacgtcctc caggaacacc tcctccggga tggtgccgtt
     4981 gtccatcgac caggtgtcga tggt
//
LOCUS       DASPJI010000191         3532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_126574, whole genome shotgun sequence.
ACCESSION   DASPJI010000191 DASPJI010000000
VERSION     DASPJI010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126574"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VEL74_05030"
     CDS             <1..894
                     /locus_tag="VEL74_05030"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYG61920.1"
                     /translation="AGRLRLLRGSLEDRDALAAAFRGARTVVHLATGGGTTWQAVERS
                     MINGSLGAAEAALAEGVERFVYVSSIAALYAGPDAGPVLEDSLAVDPKAEERDVYSRG
                     KAVAEQALLRLHRERGLPLVIVRPGVVLGQGTPLQHSGLGLWVRDNHCVGWGQGDHPL
                     PVVDVEDVGDALARLVAYEGRDLEGQALNLCTRTPLSAREIVEEMARATGRDLHFHPR
                     PLWLSQGLEIGKWLVKKVGRQKDAPFPSYRDLKTRSLAPPFTARTAREVLGWTPVEDR
                     EGFLDRAVRGSSSSPRSDGRA"
     gene            881..2068
                     /locus_tag="VEL74_05035"
     CDS             881..2068
                     /locus_tag="VEL74_05035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG61921.1"
                     /translation="MAEPDVQSVRGSVGPPEAPGGLRRPHLLHVFSNFVPTGPELRTV
                     ALIGAFGDEFRHSIVSMDGRTGAAERLPPGAPVRLLQNPPRAGSLQTARRLRRLLADV
                     GPDLVLTYNWGAFDMLLAARSRGFRRVIHHEEGFNDDETESYKLRRVLARRLVLPAAF
                     QVVVPSQRLQRVAVDLWRLPGRRVRLIPNGIRPDSFGPADGHPELRRAHGIPDGALVV
                     GAVGSLRPVKNFMRLLEAAAVASDSAPEAHVLLVGDGEERPALEARAAAPDLAGRIHF
                     AGYQADPRPFYRAMDLFAVTSDSEQMPVSLLEAMASSLPVIATDVGDVRAMLPVGQEH
                     YVVPPGPEAAAALGEGIRKLAADPAIRRRIGSANRRRVETRYSFDSMCAAYREVYHAA
                     LCS"
     gene            complement(2073..3260)
                     /locus_tag="VEL74_05040"
     CDS             complement(2073..3260)
                     /locus_tag="VEL74_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61922.1"
                     /translation="MIRRLITGPLLLILAVLAAAPAAAQLVPLGREQRVDTSATAGYC
                     PALAADAGGGFTVAWANTQDESIHSCTPAGRLYTRTYDRDGTAEAPPFLAVRAGDRCV
                     YDLRVGTAPGEEPLFTWREEAGKYGGHSVAVARSGGSEHSLRRGVLVPLPSGDFVTLL
                     RASGNGRRGVVVERRHDPEEEGRSFLAGINGGADAAEMPDGGLVIVWSQGYARRIGVT
                     VHGRRFDADGRPLGPAFLISGNDFASSNREVMVAANEAGQFVVTWTSIAGPVSSSYAM
                     ARAYGSDGQPASPVLRVSPRVEARQSQRPSRPAIDEQGRFVVLWRHYDARNPAEAKLS
                     ARMFEANGRRLGPELRLADWSPVSGCAGVASLEDGRWVMAWGRHPFYADASGIYLQRF
                     TSE"
BASE COUNT          463 a         1347 c         1236 g          486 t
ORIGIN      
        1 gccggccgcc tgcgcctgct gcgcggcagc ctggaggacc gcgacgccct ggccgcggcg
       61 ttccgcggcg cccgcacggt cgtccacctg gccaccggcg gcggcaccac ctggcaggcg
      121 gtcgagcgct ccatgatcaa cggctcgctc ggcgccgccg aggccgctct cgccgaaggg
      181 gtcgagcggt tcgtctacgt ctcgtcgatc gccgccctct acgccgggcc cgacgcgggc
      241 ccggtcctcg aagactccct ggcggtggac cccaaggccg aggagcggga cgtctacagc
      301 cgcggcaagg cggtggccga gcaggccctc ctccggctgc accgcgagcg cggcctgccc
      361 ctcgtcatcg tccgtccggg cgtggtgctc ggccagggga cgccgttgca gcactccggc
      421 ctcggcctgt gggtgcgcga caaccactgc gtcggctggg gccaggggga tcacccgctg
      481 cccgtggtcg acgtggagga cgtgggtgac gccctggcgc ggctggtggc ctacgaagga
      541 cgcgacctcg aaggccaggc gctgaacctc tgcacccgca cgccgctgtc ggcgcgcgag
      601 atcgtcgagg agatggcgcg cgccaccggc cgcgacctcc acttccatcc gcgccccctg
      661 tggctgagcc aggggctcga gatcggcaag tggctggtca agaaggtggg acgccagaag
      721 gacgcgccgt tcccgtccta tcgcgatctc aagacgcggt cgctcgcccc tcccttcacc
      781 gcgcgcaccg cgcgcgaggt cctcggctgg accccggtcg aagaccgcga ggggttcctg
      841 gaccgcgcgg tgcgcggctc ttcttcaagc cctcggagcg atggccgagc ctgacgtcca
      901 atccgtgcgc ggcagcgtag ggccgccgga ggccccggga ggcctccggc ggccccacct
      961 gctccacgtg ttctccaact tcgtccccac cgggccggag ctgcggaccg tggccctcat
     1021 cggggcgttc ggcgacgagt tcaggcattc catcgtctcc atggacgggc ggacgggggc
     1081 cgccgaacgc cttcctccag gcgcccccgt ccgtctgttg cagaacccgc cgcgcgcggg
     1141 ctcgctccag accgcccgcc gcctgcgccg gctcctcgcc gacgtcggcc cggatctggt
     1201 cctcacctac aactggggag cgttcgacat gcttctcgcc gcccgaagcc gcggcttccg
     1261 gcgggtgatc caccacgagg aggggttcaa cgacgacgag acggagagct acaagctccg
     1321 ccgcgtcctg gcccgccgcc tggtgctgcc cgccgccttc caggtggtgg tgccgtcgca
     1381 acgcctgcag cgggtggccg tcgacctctg gcggctcccc ggccggcgcg tgcgcctcat
     1441 ccccaacggc atccgccccg actccttcgg gcccgcggac ggccaccccg agctgcgccg
     1501 cgcccacggc atccccgacg gcgccctggt cgtcggcgcg gtaggttcgc tgcggccggt
     1561 gaagaacttc atgcgcctgc tggaggcggc ggctgtcgct tcagacagcg ctccggaggc
     1621 gcacgtcctg ctggtgggcg acggcgagga gcggccggcg ctcgaggcgc gggccgcggc
     1681 gccggacctc gccggccgca tccacttcgc cggctatcag gcggatcccc ggccgttcta
     1741 ccgcgccatg gacctcttcg ccgtcacctc ggacagcgag cagatgcccg tctccctgct
     1801 ggaggcgatg gcgtcgagcc tgccggtgat cgccaccgac gtgggggacg tgcgggccat
     1861 gctcccggtg ggccaggagc actacgtcgt gccgccgggc cccgaggccg ccgccgctct
     1921 gggggaggga atccgcaagc tcgcggccga tccggccatc cggcgccgga tcgggtccgc
     1981 caaccggcgc cgggtggaga cgcgctacag cttcgattcc atgtgcgccg cctaccggga
     2041 ggtctaccac gcggcgctgt gctcgtaacg ggctactcgc tcgtgaaccg ctgcagatag
     2101 atgccggagg cgtccgcgta gaaggggtgg cggccccagg ccatcaccca gcgcccgtcc
     2161 tccaggctcg ccacgccggc acagccactc acgggcgacc agtcggccag ccgcagctcg
     2221 ggtccgagac gccggccgtt ggcctcgaac atccgggcgg agagcttggc ctctgccggg
     2281 ttgcgggcgt cgtagtggcg ccagagcacg acgaatctcc cctgctcgtc gatggcggga
     2341 cggcttggtc gctgactctg cctggcctcc acccgggggc tcacccgcag gaccgggctc
     2401 gccggctggc cgtcggagcc gtaggcgcgc gccatggcat aggagctcga gaccgggccg
     2461 gcgatggagg tccaggtgac gacgaactga cccgcctcgt tggcggccac cattacctcc
     2521 cggttggagc tggcgaagtc gttgccgctg atcaggaagg ccggcccgag aggacgcccg
     2581 tcggcgtcga accggcggcc gtggacggtg actccgatcc ttctcgcgta cccctggctc
     2641 cagacgatga ccagcccgcc atcgggcatc tcggccgcgt cggcgccacc gttgatcccg
     2701 gccaggaagc tccggccctc ctcctccgga tcgtggcgcc gctccaccac cacgcctctg
     2761 cgcccgttgc cggaggcccg aagcagggtg acgaagtccc ccgacggcag cggcaccagc
     2821 acgccccgcc gcagggagtg ctccgagccc ccggagcgcg ccacggcgac gctgtggccg
     2881 ccgtacttgc cggcctcctc gcgccaggtg aagagaggct cctcgccggg cgccgtgccg
     2941 acccgcaggt catagacgca ccggtcgccg gcgcggacgg ccaggaaggg gggcgcctcg
     3001 gccgtcccgt cccggtcata ggtccgggta tacaggcgtc cggcaggggt acaggagtgt
     3061 atcgactcgt cctgggtgtt ggcccaagcc acggtaaatc cgccgccggc gtccgcggcg
     3121 agggccggac agtagcccgc cgtggcactg gtatccaccc gctgctcgcg ccccagtggg
     3181 acgagctggg ccgcggccgg cgccgccgca aggacggcga ggatgagaag aagaggtcca
     3241 gtgatcagac ggcgaatcaa gcgccacctc cggattcttg tcaaccagtc tacacgtccc
     3301 gtcgcgcgcg gctcctcctc ctcaccgccc tcctccccct ggcctgtggc gccgacgccg
     3361 agcccccggc caggcggccg gaccccggcg cctacctcgc ctacgttcgc gacgcgcccg
     3421 agttccagcc cgttccgcac gatccagcct tcgagatcgg ccgctgggac acctggctct
     3481 acatgccatg gcgctaccga tggacgatcg gcaccggcga cgagggaggg cg
//
LOCUS       DASPJI010000192         4996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_525257, whole genome shotgun sequence.
ACCESSION   DASPJI010000192 DASPJI010000000
VERSION     DASPJI010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525257"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..110
                     /locus_tag="VEL74_05045"
     CDS             <1..110
                     /locus_tag="VEL74_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019666672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG61923.1"
                     /translation="EPEELGETVAFLCSPAASYLTGQALAVDGGAVRGL"
     gene            complement(118..2592)
                     /gene="leuS"
                     /locus_tag="VEL74_05050"
     CDS             complement(118..2592)
                     /gene="leuS"
                     /locus_tag="VEL74_05050"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013614673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HYG61924.1"
                     /translation="MEKPRNPRGYDPAEIEPKWQERWEAEGLYRADVDRDRPKHYALT
                     MLPYPSGDLHIGHWYSICPVDARARFMRMRGANVLFPMGFDAFGLPAENAAIKRNIHP
                     KEWTYANIDRMRGQLRRMGTMIDWRREIVSADPEYYRWTQWLFLQFFKHGLAYRKYAA
                     VDWCPSCNTTLAREQVIGDDRRCERCDTPVIRKELEQWFFRTTRYADELLDFSRIDWP
                     ERVQTLQRNWIGRSEGAHVVFTAEIGEAGEPLEIFTTRPDTLWGATFMVLAPEHPLVA
                     RLTSDAQRAEVEAYVETATRQTDIQREAADKEKTGVWTGGWAINPVNGEKVPVWIADY
                     VLMGYGTGAIMAVPAHDERDFAFARKYGLPIRPVIRPEGAEPVSGDELTEALTHDGVM
                     VDSGPLTGTPADQAYEKVTALLEEKGVGRGAVTYRLRDWLISRQRYWGAAIPIVYCQK
                     DGAVPVPDDQLPVLLPDDIEWRPSGESPLKLHPTWSKTTCPVCGGEARRETDTMDTFM
                     CSSWYHLRYLSPRYDQGPFDPAEYDYWMPVDTYTGGIEHATMHLIYTRFFHKGLRDMG
                     IVQGDEPMLQLRNQGIILGEDSEKMSKSRGNVVAPDDLVDRYGADTVRAYLMFFARWE
                     LGGPWNSRGIEGTSRWLRRVWLMFVEAEEDGKEAAPGIAEGLWRKTQKTLKQVTRDFE
                     TLQFNTIVAALMELLNEVSQARNEGAASSPVWRNVQDVYLRMMAPVAPHAAAEMWERL
                     GFEGSVHLQDWPDVDEAAARDEEVTLVVQVNGKLRDRLTVPVDLPEEEMRQRALASEG
                     AQRFLEGMTVRKVVVVPGRLVNIVAT"
     gene            complement(2797..3045)
                     /locus_tag="VEL74_05055"
     CDS             complement(2797..3045)
                     /locus_tag="VEL74_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61925.1"
                     /translation="MKKLMQLLAVGVVVLLSTWTAAEARRICASGNSPTAECHIQCEG
                     DHPWDLDMTAQECCHHFRECCNSEGYAYFGDPQTGSCN"
     gene            3425..3913
                     /locus_tag="VEL74_05060"
     CDS             3425..3913
                     /locus_tag="VEL74_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61926.1"
                     /translation="MYALPVLRLFSRVPAYLEEQDEPITAAEAESAGVWGIERLPEGT
                     WGVVRPGDSVARGDEPEGVFHRLEHAVEAAVVFPTLGRDNLFTLHPDKTPRGYAIEMP
                     GELVGHLRHFDSSLTDRMHLADCLLRTPDSLGRLLRAAGGAALTRTDKLLLHRLFVQE
                     VP"
     gene            complement(3965..4219)
                     /locus_tag="VEL74_05065"
     CDS             complement(3965..4219)
                     /locus_tag="VEL74_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61927.1"
                     /translation="MKKLTQLLALVAVVLLSSLNTAGADIGDCHIECAYGGGSYHGPI
                     AGGYWGCCNYFGELCGYYGTAYEEGGSPLEDTYCPSMGEM"
     gene            complement(4791..>4996)
                     /locus_tag="VEL74_05070"
     CDS             complement(4791..>4996)
                     /locus_tag="VEL74_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61928.1"
                     /translation="IVLLSTLATAGAEPGDCHIECRYGGAWQGPASYGDCCQIFATAC
                     DNYGTAYLEGEQPLQHLYCPSYG"
BASE COUNT          773 a         1683 c         1678 g          862 t
ORIGIN      
        1 gagagcccga agagctggga gagaccgtcg ccttcctctg ctcccccgcc gcctcctacc
       61 tcaccggcca ggcgctggcg gtagatgggg gggcggtgag ggggttgtaa gcccccccta
      121 cgtcgccacg atattcacca gccggcccgg caccaccacc accttccgca ccgtcatccc
      181 ctccaggaag cgttgcgcgc cttcgctggc gagggcgcgc tggcgcatct cctcctcggg
      241 gaggtcgacc ggcacggtga ggcggtcgcg gagcttgccg ttgacctgga cgaccagggt
      301 gacctcctcg tccctggccg cggcctcgtc gacgtcgggc cagtcctgca ggtggaccga
      361 gccctcgaag ccgaggcgct cccacatctc cgcggcggcg tggggggcca ccggcgccat
      421 catccgcagg tagacgtcct ggacgttcct ccagaccggc gacgacgcag ccccctcgtt
      481 cctcgcctgc gacacctcgt tgagcagctc catcagggcc gccacgatgg tgttgaactg
      541 gagcgtctcg aagtcgcggg tgacctgctt gagcgtcttc tgggtcttgc gccagagccc
      601 ctcggcgatc ccgggagcgg cctccttccc gtcctcctcc gcctcgacga acatcaacca
      661 gacgcggcgc agccagcgcg aggtcccctc gatgccccgg ctgttccacg gcccgccgag
      721 ctcccagcgg gcgaagaaca tgagataggc gcgcaccgtg tcggcaccgt agcggtccac
      781 caggtcgtcc ggcgccacca cgttgccgcg gctcttcgac atcttctccg agtcctcgcc
      841 caggatgatc ccctggttgc ggagctggag catcggctcg tcgccctgga cgatccccat
      901 gtcccgcagc cccttgtgga agaagcgggt gtagatcagg tgcatcgtcg cgtgctcgat
      961 gccgccggtg taggtgtcga ccggcatcca gtagtcgtac tcggcggggt cgaacggccc
     1021 ctggtcgtag cgggggctca ggtagcgcag gtgataccag gacgagcaca tgaacgtgtc
     1081 catggtgtcc gtctcgcgcc gcgcctcgcc gccgcagacc gggcaggtgg tcttggacca
     1141 cgtcgggtgg agcttgagcg ggctctcccc cgagggccgc cactcgatgt cgtccggcag
     1201 cagcaccggg agctggtcgt ccggcaccgg caccgcgccg tccttctggc aatagacgat
     1261 cgggatggcc gccccccagt aacgctggcg cgagatcagc cagtcgcgca accggtaggt
     1321 cacggcgccg cggccgaccc ccttctcctc gaggagcgcc gtcaccttct cataggcctg
     1381 atcggccggc gtgccggtga gcggtccgga gtccaccatg accccgtcgt gggtgagcgc
     1441 ctcggtcagc tcgtcgccgg agaccggctc ggccccctcg ggccggatca ccgggcggat
     1501 gggcagcccg tacttccggg cgaacgcgaa atcgcgctcg tcgtgggccg gcacggccat
     1561 gatggcgccg gtgccgtagc ccatcagcac gtagtcggcg atccacaccg gcaccttctc
     1621 gccgttgacg gggttgatgg cccagccgcc ggtccagacg cccgtcttct ccttgtcggc
     1681 cgcctcgcgc tggatgtccg tctgccgcgt ggccgtctcg acataggcct cgacctcggc
     1741 gcgctgggcg tcgctggtga gcctggcgac gagcggatgc tcgggggcca gcaccatgaa
     1801 ggtggcgccc cagagggtgt ccggccgggt ggtgaagatc tccagcggct cgccggcttc
     1861 gccgatctca gcggtgaaga cgacatgggc cccttcggag cggccgatcc agttgcgctg
     1921 gagcgtctgg acgcgctcgg gccagtcgat cctcgagaag tcgagcaact cgtcggcata
     1981 gcgggtggtc cggaagaacc actgctccag ctccttgcgg atgaccggcg tgtcacagcg
     2041 ctcgcagcgg cggtcgtcgc cgatgacctg ctcgcgggcg agggtcgtat tgcagctcgg
     2101 gcaccagtcg acggcggcgt acttcctgta ggcgaggccg tgcttgaaga actggaggaa
     2161 cagccactgg gtccaccggt agtactcggg gtcggccgag acgatctcgc ggcgccagtc
     2221 gatcatcgtg cccatgcggc ggagctggcc gcgcatgcgg tcgatgttcg cgtacgtcca
     2281 ctccttgggg tggatgttgc gtttgatcgc ggcgttctcg gcgggcaggc cgaacgcgtc
     2341 gaagcccatg gggaacagga cgttggcgcc gcgcatccgc atgaaccggg cgcgggcgtc
     2401 caccggacag atggagtacc agtggccgat atggagatcg cccgaggggt acggcagcat
     2461 ggtgagcgcg tagtgcttgg gccggtcgcg gtcgacgtcg gcccggtaga ggccctcggc
     2521 ctcccaccgc tcctgccact tcggttcgat ctcggccggg tcgtatcccc ggggattcct
     2581 gggcttctcc atgtccagcg tccttatcta tagatgtcgc agctgtcgcc gccggggccc
     2641 cggcgcggaa gagggtgagc ttatggcgcg gggggggtgg ggtgcaaggg ccgcccacca
     2701 gggagaacag agggggattc ccggcttgaa gggctctgag gttcggggca accggctcgc
     2761 gaggctgcgg gctcgcgagc cgggtctctc ggcgctctag ttgcaggagc cggtctgagg
     2821 atctccgaag taggcatagc cttcggagtt gcagcactcg cggaagtgat ggcagcactc
     2881 ctgggcggtc atgtcaaggt cccacggatg gtcgccctcg cattggatgt ggcactcggc
     2941 agtcgggctg ttgccggaag cgcagatgcg gcgcgcctcc gctgcggtcc acgtggagag
     3001 caggacgacg actccgacag ccagtaactg catcagcttc ttcatctggt tttctcctct
     3061 aaggccccgc cagggatggc caagtcggtt caagagcaca aaatcttagc atattagatt
     3121 cagtgctcac tgtccccgcc tcgcccggcc agcctcctca ggggatcgtc caggcgacta
     3181 tcgctctatc ccttgatttc cgcctcaccc tcttataggc ttcttccagg acaaagctct
     3241 gccgtaccga gcctggcatc ctgaaaggag gtgcagcgcg gttcaacctg gaagcgatct
     3301 tcgcggttcg tgacaagcgg ccggcgtgtg tccggccatc cattcaagcg gcccggagtg
     3361 agtcttgacc ctcaccccgg accatagcct cgcaaagggg gtaaccaatg cgaagccgta
     3421 gtgattgtac gccttacccg ttctccgtct tttctcccgc gttcctgcat atctggagga
     3481 acaggacgag cccatcacgg cggccgaggc cgaatcggcc ggggtgtggg ggatcgagcg
     3541 tctgcccgag ggcacctggg gggtggtccg tcccggggac agcgtggcgc ggggggacga
     3601 gccggagggg gtcttccatc gcctcgagca cgccgtcgag gccgcggtgg tcttcccgac
     3661 cctcggccgg gacaacctct tcaccctcca ccctgacaag acgccccgcg gctatgccat
     3721 cgagatgccc ggcgagttgg tggggcacct tcgccacttc gactccagcc tcaccgaccg
     3781 gatgcacttg gcggactgcc tgctgcgcac gcccgactcg ctcggccggc tcctccgcgc
     3841 cgccggcggc gccgccctga cccggacgga caagctgctg ctccaccgcc tcttcgtgca
     3901 agaggtaccc tgacgttttc ggcgccggct cgcgagattc gcgggccggc gccacctggc
     3961 ccgcttacat ctcgcccatg ctcgggcagt acgtgtcttc gaggggggac ccgccctcct
     4021 cataggcggt accgtagtag ccgcaaagct cgccgaagta gttacagcac ccccaatatc
     4081 ccccggcgat ggggccatga tacgagccac cgccgtaggc gcactcgatg tggcagtccc
     4141 cgatgtcggc tccggcggtg ttgagagagg agagcaggac gacggccacg agcgccagca
     4201 actgcgtcag cttcttcata gagctcctcc tgaaaaggcc cgctcctgag gcttggcgag
     4261 ccggatggat ggttcgccaa gtttaactgc atagatggaa acacgaagcg gcggcttcgg
     4321 gggcggaggt ggacctcccc ggggaggcgc tggtccggga atccggcccc cctttgacat
     4381 cgcacgctga aagactgaag atccgctctt cacccctccg ccctgcgatg ggcagaaagg
     4441 acacgacctg atgaggaagc ggacgaagaa gctctcgttg tcgaaggaga ctctggctca
     4501 catggatgcc cggaggctgg agggggccgc cggcggttcg atcaatcctc cctttgaaac
     4561 cgacaccatc atcccggact gcgcgacgcg caacgtcagt tgtcagggca gttgtgcctg
     4621 caccgggacc gagctttgtc agactgtgac ctgcacttgc tgaagcggcg caaacgcctc
     4681 tgagatagct ggtccggtca ggtcgctggg cgcgcttcag cagcggtcca tgtggcgcga
     4741 gctggccgga tcccatctcc cccgcccgga gcgcttccct gctcccgcgc ctaaccgtag
     4801 ctcgggcagt agagatgttg caagggctgc tcgccttcga ggtaggcggt gccgtagttg
     4861 tcgcaagcgg tagcgaagat ctggcaacag tcgccgtagg acgcgggccc ctgccaggca
     4921 ccgccgtacc tgcactcgat gtgacaatct cccggctccg ctccggccgt ggccagggtg
     4981 gagagcagga cgatgg
//
LOCUS       DASPJI010000193         4333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_126634, whole genome shotgun sequence.
ACCESSION   DASPJI010000193 DASPJI010000000
VERSION     DASPJI010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126634"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VEL74_05075"
     CDS             complement(<1..569)
                     /locus_tag="VEL74_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYG61929.1"
                     /translation="MTLYTIPDLRYERYTLSNGLRVVLHRDTSLPLVTVNLWYHVGSK
                     NERPGRTGFAHLFEHMLFQGSQHVPTNDHFRLVQQVGGVANGSTWYDRTNYYETLPAH
                     HLDLGLWLESDRMGFFLPALTQENLDTQRSVVMNERRQRVENQPYGMAGERIHELLYP
                     DDFPYHWPVIGYMEDIAAANLEDVRTFFQT"
     gene            700..1314
                     /gene="lexA"
                     /locus_tag="VEL74_05080"
     CDS             700..1314
                     /gene="lexA"
                     /locus_tag="VEL74_05080"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HYG61930.1"
                     /translation="MDYLTERQKEILEFIHHFRREKGIAPTHREICERFGFSSYGTAY
                     KHLKLLQEKGYLRRDWNQKRGIELLRAIPGGEMTARQIPFYGRIAAGRPLEAMPGNER
                     VAVPDSLLAGKEGEHYVLRVVGESMIEEGIQDGDYVIVLRREVAQPGEMVVALVGDDA
                     TLKRFYPEGKTVRLQPANPNMQPIRVPARDVRVQGIVVGLMRKF"
     gene            1450..1983
                     /locus_tag="VEL74_05085"
     CDS             1450..1983
                     /locus_tag="VEL74_05085"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011730198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HYG61931.1"
                     /translation="MSDLRFALHRDALGFAYAVARGGSLVGLGHRPSLEEAEQSCARS
                     WPGAVRDDAVRDDLAPPFPQLWDQLDEYLEGRRRSFDLPLAPAGTEFQRLCWQALREI
                     PYGQTRSYSEQARLIGRPAAIRAVGRANHDNPIGVVIPCHRVIGANGHLTGYAGGLPM
                     KRLLLELEGVLTKSLAL"
     gene            2054..3493
                     /locus_tag="VEL74_05090"
     CDS             2054..3493
                     /locus_tag="VEL74_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HYG61932.1"
                     /translation="MDLDRLLKFMTDKGASDLHLKPTRPPLLRVTGRLLPIETEPLTP
                     EDLSHMLLKILTPQQKARLDERMSVDIGYGVRGLARFRGNIYMQRGTLAASFRRVPYQ
                     IKTLEDLDLPQVLHEFCDLPMGLVLVTGPTGSGKSTTLAAMIKHIAARRPCHVITIED
                     PMEFLFTDDMASISQREVGTDTTAFSEALRNAMRQDPDVIMVGEMRDPETVATVITAA
                     ETGHLVFSTLHTNSAPQTIDRILDTFPAEQQGQIRAQLAQILKGVVTMKLVERQDGQG
                     LVAALEILRASPKVCKMIEAGEISAIHEEMESSVGYYRMQSMNHSLLALLVHGTISYA
                     EAMRQSPDPEDLSLKLRKMFPMIEERGGELSPTTSDFSQIVELQQFKKLYEEQEEKVR
                     LRLAEKDEQMNELRAHIQSRDGQIEEMGQRLQELSQDRERIRGDYARLRQEAQEKIDK
                     LMERIKELNARLMGGGGDTGERKTGIFGR"
     gene            complement(3839..4132)
                     /locus_tag="VEL74_05095"
     CDS             complement(3839..4132)
                     /locus_tag="VEL74_05095"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYG61933.1"
                     /translation="MIRIRLSSAASEDVREAQAWYAGQATGLDLEFRRELDRVLERIR
                     TYPAGFPQIHRSIRRANLRRFPYGVFYVRRDDHLFVLAVAHHARSPSHWKRRL"
     gene            complement(4129..>4333)
                     /locus_tag="VEL74_05100"
     CDS             complement(4129..>4333)
                     /locus_tag="VEL74_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HYG61934.1"
                     /translation="LERQVLDLPEEERLEIAETIWSSLRDPDALPLPAWQRDFLDERL
                     ASSEGEEGRDWEEVRAEIWPDAQ"
BASE COUNT          745 a         1450 c         1449 g          689 t
ORIGIN      
        1 gtctggaaga aagttcgtac gtcctccaga ttggccgcgg cgatgtcctc catgtagccg
       61 atgaccggcc agtgataggg gaagtcgtcc gggtacagca gctcgtggat gcgctcgccg
      121 gccatgccgt acggctggtt ctcgacacgc tgccgccgct cgttcatcac cacggagcgc
      181 tgggtgtcca ggttctcctg ggtgagggcg gggaggaaga agcccatgcg gtccgactcg
      241 agccagaggc cgaggtccag gtggtgggcc ggcagggtct cgtaatagtt ggtgcggtcg
      301 taccaggtgg agccgttggc caccccgccc acctgctgga cgagccggaa atggtcgttg
      361 gtcgggacgt gctggctccc ctggaagagc atgtgctcga acaggtgggc gaagccggtg
      421 cggccgggcc gctcgttctt cgagccgacg tggtaccaga ggttgaccgt gaccaggggg
      481 agggaggtgt cgcgatgaag gacgacccgt aatccgttgc tcagggtata tcgttcgtag
      541 cgaaggtcag gtatcgtata tagagtcatc agtcgagccc cgctggggga gagggtaacc
      601 gcccccgtca gggtgccgag gcagtgtatg gaggggtgtc gaagcgtgtc aagaacaaca
      661 acctattgca ttacgctcgg attgcgctaa actatggcca tggactactt gaccgaacgt
      721 cagaaagaga tcctcgaatt catccaccac ttccgccggg agaagggtat cgctcccacc
      781 caccgtgaaa tctgcgagcg cttcggcttc tcgtcgtacg gcaccgccta caagcacctc
      841 aagctcctcc aggagaaggg ttacctgcgg cgggactgga accagaagcg cggcatcgag
      901 ctgctgcggg cgatccccgg cggcgagatg acggcgcgac agatcccatt ctacggccga
      961 atcgccgccg gccgacccct cgaggcgatg ccgggcaacg agcgcgtcgc cgtccccgac
     1021 agcctgctgg ccggcaagga gggggagcac tacgtcctcc gcgtcgtcgg cgagtcgatg
     1081 atcgaggagg ggatccagga cggcgactac gtcatcgtgc tgcggcgcga ggtcgcccag
     1141 cccggcgaga tggtcgtcgc gctggtcggc gatgacgcca ccctcaagcg tttctatccc
     1201 gaagggaaga ccgtccgtct gcagccggcg aacccgaaca tgcagccgat ccgcgtcccg
     1261 gcccgggacg tgcgggtgca gggcatcgtc gtcggcctga tgcggaagtt ctgaccggcc
     1321 cccggcggcc tcggctgccg ggcccgcgtc tccccgcccc gggaaaggag aacgatcgat
     1381 cggggcgggg acggggaccg gtgcgccgcg tcaggaggcc gccgtggaga ccgtgccacg
     1441 aggtgagata tgtctgacct gcgtttcgct ctgcatcgcg acgccttggg cttcgcctac
     1501 gcggtggccc ggggcggctc cctcgtcggc ttgggtcacc gcccctcgtt ggaggaggcg
     1561 gagcagtcgt gcgccagatc gtggcccggt gcggtgcggg atgacgcggt gcgggacgat
     1621 ctggcgcccc ctttcccgca gctctgggac cagctcgacg agtacctcga ggggcggcgg
     1681 cgctccttcg atctccccct cgccccggcg ggcaccgagt tccagcgcct ctgctggcag
     1741 gcgctgcggg agatccccta cggccagacc cgctcctatt cggagcaggc ccgcctcatc
     1801 ggccgccccg cggcgatccg ggcggtgggc cgggcgaacc acgacaaccc catcggcgtc
     1861 gtgatcccct gccaccgcgt catcggcgcc aacggccacc tcaccggcta cgccggcggc
     1921 ctgcccatga agcgtctcct cctggagctg gagggggtat tgacgaagtc gctggccctc
     1981 taggccgtgc gccgggctag ttcagccctc gctcctcctg tacaatgagc cccccaaaga
     2041 ctcaaaggga cctatggacc tcgaccgtct actgaaattc atgacggaca agggggcttc
     2101 cgacctccat ctgaagccca cccgtccgcc tttgctccgg gtcaccggcc ggctgctccc
     2161 catcgagacg gagccgctga cgccggagga cctgtcccac atgctgctca agatcctgac
     2221 gccgcagcag aaggcgcggc tcgacgagcg catgtcggtc gacatcggct acggcgtgcg
     2281 cgggctcgcc cgcttccgcg gcaacatcta catgcagcgc ggcacgctcg ccgcctcgtt
     2341 ccggcgcgtg ccgtaccaga tcaagaccct ggaggacctc gacctgccgc aggtgctcca
     2401 cgagttctgc gacctgccca tgggtctggt cctggtgacc ggccccaccg gcagcggcaa
     2461 gtcgacgacg ctcgcggcga tgatcaagca catcgcggcg cggcgcccct gccacgtcat
     2521 caccatcgaa gacccgatgg agttcctgtt caccgatgac atggcctcga tctcccagcg
     2581 cgaggtgggg accgacacga ccgccttctc cgaggcgctg cgcaacgcca tgcggcagga
     2641 cccggacgtc atcatggtgg gcgagatgcg cgacccggag acggtggcga cggtcatcac
     2701 cgccgccgag accggccacc tcgtcttctc gacgctgcac accaacagcg cgccgcagac
     2761 catcgaccgc atcctcgaca ccttccccgc cgagcagcag gggcagatcc gggcgcagct
     2821 cgcgcagatc ctcaagggtg tggtgacgat gaagctggtg gagcgccagg acggccaggg
     2881 cctggtggcg gcgctggaga tcctccgcgc ctctcccaag gtctgcaaga tgatcgaagc
     2941 cggcgagatc agcgccatcc acgaggagat ggagtcctcg gtcggctact accgcatgca
     3001 gtcgatgaac cactcgctgc tggcgctgct ggtccacggc accatctcct atgccgaggc
     3061 gatgcgccag tcgcccgacc cggaagacct ttcactgaag ctccgcaaga tgttccctat
     3121 gatcgaggag cgaggaggcg agttgagccc gaccacgtcc gacttttcac agatcgtcga
     3181 gctccagcag ttcaagaagc tgtacgagga gcaggaggag aaggtgcgcc tgcggctggc
     3241 cgagaaggac gagcagatga acgagctgcg cgcccacatc cagtcgcgcg acggccagat
     3301 cgaggagatg gggcagcgcc tccaggagct gtcccaggac cgcgagcgca tccgcggcga
     3361 ctacgcccgc ctgcgccagg aggcccagga gaagatcgac aagctcatgg agcgcatcaa
     3421 ggagctcaac gcccgcctca tgggcggcgg cggcgacacc ggcgagcgca agaccgggat
     3481 cttcgggcgc tagcccgcgg ctctcacccc cccggccccc tctcccgctc accgggggcc
     3541 ggccccctcc cttccgcgag gatttttcgt cgcagatcag ggttcccgcg ggtctcgacc
     3601 cggggagaac gtcgccgagc ggcggcgctt cgtcgcagat ccgcaccact ctgcgacgaa
     3661 cgaagaccgc tctgcgacga tgggacgttc atctgcgacg attgaaggct gatccgcgac
     3721 gaatgaacgc tggaacgcga tgatccggag ccgatctgcg acgtttcgag gcagctctgc
     3781 gacgtttggc tcggcgccgg aggcttgcct cggatgaccg tgggctaggt tcaactccct
     3841 agagccggcg cttccaatgg ctcggggaac gggcgtggtg ggcgacggcc aatacgaaga
     3901 ggtgatcgtc gcgcctgacg tagaagacgc cataggggaa gcggcgcagg ttggcgcggc
     3961 ggatggagcg gtggatttgt gggaaaccgg ccggatatgt ccgaatccgc tccaagactc
     4021 tatccagctc ccgcctaaac tctagatcca gtcccgtggc ttggccggcg taccaggcct
     4081 gagcctcgcg gacatcctca ctggctgcac tgctgaggcg aatgcggatc actgggcgtc
     4141 cggccagatc tcggcgcgga cctcctccca gtcccgcccc tcctcgcctt cactactggc
     4201 caagcgctcg tccaggaaat cgcgttgcca ggccgggagc ggcagggcgt ccgggtccct
     4261 gaggctggac cagatcgtct ccgcgatctc caggcgctct tcttcgggca gatccaggac
     4321 ttggcgttcg agc
//
LOCUS       DASPJI010000194         3457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_303143, whole genome shotgun sequence.
ACCESSION   DASPJI010000194 DASPJI010000000
VERSION     DASPJI010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303143"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VEL74_05105"
     CDS             complement(<1..1014)
                     /locus_tag="VEL74_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61935.1"
                     /translation="MDEPTQTREGEPRPSRRRRKLLLLALALVALPLLVLIAALGSLR
                     VDSVRQAVLGRLSSYLAEEFKLAFSARDFDVGWGGRFEITGVRVGAPGAPPLVTAERV
                     RGAVDLWTLRSPVLGLRYVEVESPRLDLAAPIPEVPSDEPSGPPGFEIRRLSVRDGAI
                     VGAPTEGALAEWVTAWRADGLGASGSFRDGVWDVNVEQGRATVERPGFSPLVARLAGR
                     LSYKDGAPVTFRDVRAEGDGLRLAASGSVGLDPGASSSGVAATFDATVEPRLLTAGTP
                     PGGRIHARGDVRLPEATGRVSLTAEAVPAEVARPYLDGELFADLSLAGTVADARADLT
                     AGPG"
     gene            complement(1088..1270)
                     /locus_tag="VEL74_05110"
     CDS             complement(1088..1270)
                     /locus_tag="VEL74_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61936.1"
                     /translation="MKKAKKLVLSRETLRPLESHVTGAAIQPVALSDGAYQCPMTGPS
                     CCCTMGCSVELCTTLG"
     gene            1346..2815
                     /locus_tag="VEL74_05115"
     CDS             1346..2815
                     /locus_tag="VEL74_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61937.1"
                     /translation="MKALRPTPGDLAVFAVLAGLAVLVGVGFWGYSYDDAFITYRYAR
                     NFAEHGELVFNPGERVLGTTAPGYALLLGALTFLTGISVPGWGTLISLASIGAVALVL
                     RLALADAPPGVRYGAPLLYGTAAFLFRWNVEMFGAESFPVLALGVGAAWLALGDEEGR
                     RREVAAGLLIALAMIFRLDAALLAGILGLALWWRRRRFPLAFALAGLVPVLLFLLYLH
                     LTFGAVIPNTMAAKQSELPAAYSYTLEEWRWLGRSLTFVGRLSLLLAVLGAVLFGSLP
                     LARRRGAAGAVALVLILWLAAHELAYRVLAVPFAPWYHVPMINGLLAAAALGGTAWSW
                     DERRAGARWGSFGMLLLLFPLLVQSAHYAVRQWGQAPDPRWSGYMEAARRVRDGGGRS
                     VAAVEIGFLGYESDARVLDLMGLVSPEALTARRDGTLPALVARERPDYLVDVSLFQPV
                     LGPILDEPAVQASYQPVAELPDGRGPGVRLRLLRKSLTP"
     gene            complement(3158..>3457)
                     /locus_tag="VEL74_05120"
     CDS             complement(3158..>3457)
                     /locus_tag="VEL74_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61938.1"
                     /translation="WLALVAAAVLPGTGRDPLIRIDKQPDADGVYPLLLPGLGGDEMA
                     GRLRLFDEVLVDGMNVAIHLLQAPESLAWLLEAAGAVALERCGAILDERVAAVME"
BASE COUNT          476 a         1184 c         1257 g          540 t
ORIGIN      
        1 gcccgggccg gcggtgaggt ccgcccgggc gtcggcgacc gtgccggcga gggagaggtc
       61 ggcgaagagc tccccatcga ggtaaggacg ggcgacctcg gccggcacgg cctcggccgt
      121 gaggctcacc cggccggtcg cctccggcag ccggacgtcg ccgcgggcat ggatccgtcc
      181 ccctggcggc gttccggccg tgaggaggcg gggctcgacg gtggcgtcga acgtggcggc
      241 gaccccggaa gacgacgcgc cggggtcgag acccaccgag cccgaggcgg cgaggcggag
      301 gccgtccccc tcggcccgga cgtcccggaa ggtcaccggt gccccatcct tataagaaag
      361 gcgtcccgcg agccgtgcca ccagggggga gaacccgggc cgctccacgg tcgcccgtcc
      421 ctgctcgaca ttcacgtccc acacgccatc ccggaacgag ccgctggcac cgagcccgtc
      481 cgcccgccaa gccgtcaccc actcggccag agctccctcg gtcggtgcgc cgacaatggc
      541 gccgtcgcgc acggagagac ggcggatctc gaacccgggc ggacccgaag gctcgtcgga
      601 cggcacctcg gggatgggcg cggcgaggtc gagacgcggg gactcgactt ccacgtagcg
      661 caggccgagc acggggctgc ggagcgtcca gaggtcgacg gcgccccgga cgcgctcggc
      721 ggtgacaaga ggaggggcgc cgggagcgcc gacgcgcacg ccggtgatct cgaaccggcc
      781 gccccagccg acgtcgaagt cccgcgcgga gaaggcgagc ttgaattcct cggccaggta
      841 ggacgacagg cgtcccagca cggcctggcg gacggagtcg acgcgcaggc tgccgagggc
      901 ggcgatcagg acgaggaggg ggagggcgac gagcgccagg gcgagaagca ggagcttccg
      961 gcggcgtcgg ctaggacgag gttccccctc ccgcgtctgt gtcggctcgt ccatggccgg
     1021 gatagtttaa gccccgggag agacaggggc ggacaagggg ggcggatctc cgcccccctt
     1081 ggaaggacta accaagggtg gtgcagagct cgacgctgca gcccatggtg cagcagcagc
     1141 tcgggccggt catcgggcac tggtacgcgc catccgaaag ggccactggc tggatggctg
     1201 ctccagtgac atgactctcc agcggccgca gggtttcacg ggaaaggacg agcttcttcg
     1261 ccttcttcat tctgtgctcc tcttgagtga gggcagattg taacccgggg tgctagcatt
     1321 cggcctctcc gcgagcccat cgggaatgaa agcactcagg cccaccccgg gcgacctcgc
     1381 cgtcttcgcc gtcctggccg ggctcgcggt cctggtgggg gtgggcttct ggggctattc
     1441 gtacgacgac gccttcatca cctaccggta cgcgcgcaac ttcgccgagc acggcgagct
     1501 ggtcttcaac cccggggagc gggtactggg gaccacggcg ccgggctacg cgctgctcct
     1561 cggagcgctc accttcctga ccgggatctc ggtgcccgga tgggggacgc tgatctccct
     1621 cgcctccatc ggcgccgtgg cgctggtcct gcggctggcg ctggccgacg cgccgccggg
     1681 ggtgcgctac ggagcgccgc tgctctacgg cacggcggcc ttcctcttca gatggaacgt
     1741 agagatgttc ggcgccgagt ccttccccgt cctggcgctg ggggtggggg cggcgtggct
     1801 ggccttggga gacgaagagg ggaggcgccg cgaggtcgcc gccgggctgc tgatcgccct
     1861 cgcgatgatc ttcaggctcg acgcggctct cctcgcgggc atcctggggc tcgcgctgtg
     1921 gtggaggcgg cggcgtttcc cgctggcgtt cgctctggcc gggctggtgc cggtgctgct
     1981 cttccttctc tatctgcatc tcaccttcgg agccgtgatc cccaacacca tggcggccaa
     2041 gcagtccgag ctgcccgcgg cctactccta caccctggaa gagtggcgct ggctgggccg
     2101 ctccctgacc ttcgtgggac gtctctccct cctgctggcg gtgctgggag cggttctctt
     2161 cggttccctg cccctggctc ggcggcgggg cgcggcggga gcggtggctc tggtgctgat
     2221 cctctggctc gccgcgcacg agttggctta ccgggtcctc gccgtcccct tcgccccctg
     2281 gtaccacgtg ccgatgatca acggcctgct ggcggcggcg gccttggggg ggaccgcctg
     2341 gtcgtgggat gagcggcgcg cgggcgccag gtggggaagc ttcgggatgc tgctcctcct
     2401 cttccccctg ctcgttcagt ccgcccacta tgccgtgcgc cagtggggcc aggcgccgga
     2461 ccctcgttgg agcggctaca tggaggccgc gcgccgcgtc cgggatgggg gagggaggtc
     2521 ggtggccgcg gtggagatcg gcttcctggg ctacgagtcg gacgcccggg tcctcgacct
     2581 catgggcctg gtgagccccg aagccctcac cgcccgccgc gacggcaccc tcccggccct
     2641 ggtcgcccgc gagaggcccg actacctggt cgacgtcagc ctgttccagc cggtcctcgg
     2701 ccccatcctc gacgagccgg ccgtacaggc gtcgtatcag cccgtggcgg agctgccgga
     2761 cggaaggggc ccgggggtgc ggctgcggct gttgcggaaa tccctcaccc cctgaggccc
     2821 cgtctccacg catccgggaa cggcgttcat tcctcccggg gcagccattg ccccggtagg
     2881 aatggttgcc atttccaacg aaatggaggc cgtttccatc gaaacgacgc tcgtttctga
     2941 aggaatggcc tccatccctg ccgaaatggc cttagtttcc ctgggaatgg ccgtcatttc
     3001 cctgggaatg gtggacgttt cccgaggaat ggtggccgtt tctcgaggaa tgaaggacgt
     3061 ttcttcggga atggtggccg tttcgggccg gatggagacc gggcgccacc ggtaaggctt
     3121 tgcccgccgg tggcgccact ccttctttat ggatgcccta ctccatgacc gcggctaccc
     3181 gctcgtcgag gatggcgccg caccgctcga gggcgaccgc tccggccgcc tccagcaacc
     3241 aggcgaggga ctccggcgcc tgaagcaggt ggatggcgac gttcatgccg tcgacgagaa
     3301 cctcatcgaa gagccggagc cggccggcca tctcgtcacc cccgagaccg gggaggagga
     3361 ggggatagac cccgtccgca tccggctgct tgtcaatgcg gatcagcgga tcgcgccccg
     3421 tgccggggag cacggcggcg gcgacgaggg cgagcca
//
LOCUS       DASPJI010000195         5747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_184943, whole genome shotgun sequence.
ACCESSION   DASPJI010000195 DASPJI010000000
VERSION     DASPJI010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184943"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VEL74_05125"
     CDS             complement(<1..515)
                     /locus_tag="VEL74_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYG61939.1"
                     /translation="MIRPEPATASALLEIEGLEVAFPARGSASVDGGWVTAVRGVSLT
                     LQRGEMLGLVGESGSGKSLTALALLGLVPPPARVRGHVWLEGEDVLALPGRALRRVRG
                     GRIAIVFQEPMTAFNPVYSIGFQIAEAVRAHRRMSRRQALDEAARLLDLVAVPDARRR
                     LGDYPHQLSGG"
     gene            complement(553..2028)
                     /locus_tag="VEL74_05130"
     CDS             complement(553..2028)
                     /locus_tag="VEL74_05130"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HYG61940.1"
                     /translation="MTRIASVTVAPPESLEADLLLVGCFEGEAPEVDALEEGVRGAVE
                     RLAGRSGWRGREEQAAQTETGPGGMVISLNGLGARRELTWAKLARWLFQAPEISRLTG
                     ARRLAFLLPAHAETTGGEAAERITRTLALTGYRYDRYLNQARERERPERIAVIPPAGQ
                     EGAFQSALATSLPLAEAVAYSRDLANSPPNEANPAWMEERARELGAERGFEVTVLDQE
                     ELARRGMGGLLAVGSASSTPPRLVRLTWGTGGPRVALVGKGVTFDSGGISIKPAADMD
                     EMKFDKSGACSVLGAARAIADLELPVRLSVYVPLAENLLDGNSYRPGDIVRCYNGKTV
                     EITNTDAEGRMILADAMAWAVEDGTDTLIEYSTLTGACVVALGHQAAGLFTPDDKLAN
                     DLLAAAGESGERLWRLPLYPEYLEEMKGSHADLKNSAGRWGGACTAAAFLSQFVGDVR
                     SWAHLDIAGVAYLGNDQDGKKKGATGYGIPTTIAWLRRLVA"
     gene            2235..4316
                     /locus_tag="VEL74_05135"
     CDS             2235..4316
                     /locus_tag="VEL74_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61941.1"
                     /translation="MNPTSVLQLLQGPFNFLRQEPEEILRLMPDLQKALPTPRPIYLE
                     DETAKPLLGFPVLLSEGMEALRQALRQYIAAEEAVQVAMVRRDTAFDRKAYNAAWERY
                     RSLLSRAVENATTSSYGRQYPAIFWLHHSLEVSRTLKDTPKRILRLDTEIGRRQGDAI
                     KYRVYDRFLDRVLATTYEVVQKVAGATEEVEEELFPRLLTRMRDNVLIFTEDYIGRDL
                     AELGSYFAGSLRIDGRDLRQRLEELVRWHSEQLAADNELRSLAIHVAGMDPTKEARDL
                     LNRPGWVTFLSGRKGYNAAKLLSPPLVQVWESLLIKLKEFELMHGLRRALVTVEMRGG
                     AIDLDGTFGGIRMAPMVARDIRSSAGGEVRLSSATRPLDFMEAWVVDPRVERFGMIYD
                     ISDFSQTLSMLHRGGNESQDEAFRRMFRFQRRINRLAHSHRAKLEKYLGDGAFYSSRE
                     ATNLLVCSVHLQRYYEQAVREGLPFDRGMRIALNYGSYRMIPMGARPEEGERYEFFGP
                     GLVELSRLTTGKATQEIDEVKTMLVSQGYPEATVHRFFAPLASRNVDVVDKREEARRF
                     YCYINRNGTLHNNGMVGTGPFVAELEKELRSPRLRRGTDGDRIYVLLEVEHAGTPMTV
                     GFRKLGFAHLKGLEKLAVYEIVDGATIDPASYADLPGASLSAAIEREFSSTGRTPGAS
                     QDSGVSPGLAY"
     gene            complement(4595..4930)
                     /locus_tag="VEL74_05140"
     CDS             complement(4595..4930)
                     /locus_tag="VEL74_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HYG61942.1"
                     /translation="MNPTDRARQLRQRMTDAERHLWAYLRRGFLNNRFRRQAPIGKYI
                     VDFVCFERKLVIEVDGGQHMGSVSDAVRDQFLREEGFLVLRFWNHEVLGNVEGVLEVI
                     GGELNRWGR"
     repeat_region   5086..5736
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5164..5200
                     /rpt_unit_seq="gtcgcaatcccaccatacagatcagggcaattccgag"
BASE COUNT          950 a         2049 c         1863 g          885 t
ORIGIN      
        1 tgcccgcccg agagctggtg cggataatcc cccagccgcc gccgcgcgtc cgggaccgcc
       61 acgagatcga gcagccgggc cgcctcgtcc agcgcctgcc ttcgcgacat ccgccggtgg
      121 gcccgcaccg cctcggcgat ctggaagccg atgctgtaga ccggattgaa cgcggtcatc
      181 ggctcctgga agacgatggc gatgcggccg ccgcgcaccc ggcgcagcgc ccgcccgggg
      241 agcgccagga cgtcctcccc ctccagccag acgtggccgc gcacccgggc cggcggcggc
      301 acgaggccga ggagggcgag cgcggtcagg ctcttgcccg agcccgactc cccgaccagc
      361 cccagcatct ccccccgctg cagggtcagg gagacgccgc gcaccgccgt cacccagccg
      421 ccgtccacgg aggcggagcc gcgggcaggg aacgccacct ccaggccctc gatctcgagg
      481 agggcgctgg cggtggcggg ctcggggcgg atcacggtct cggggagagg gcgcggcgcc
      541 ggcggccccg ggtcaggcca ccaggcgccg gagccaggca atggtcgtgg ggatgccgta
      601 gccggtggca cccttcttct tgccgtcctg gtcgttgccc agataggcga cgccggcgat
      661 gtccaggtgc gcccaggagc ggacgtcgcc cacgaactgg gagaggaagg ccgcggcggt
      721 gcaggcgccg ccccagcggc cggcggagtt cttgaggtcg gcgtgcgagc ccttcatctc
      781 ctccaggtac tcgggataga ggggcaggcg ccagaggcgc tcgcccgact cgccggccgc
      841 cgccagcagg tcgtttgcca gcttgtcgtc gggggtgaac aggcccgccg cctggtggcc
      901 gagggcgacg acgcaggcgc cggtcagcgt cgaatactcg atcagggtgt cggtgccgtc
      961 ctccaccgcc caggccatgg cgtcggcgag gatcatccgc ccctcggcgt cggtgttggt
     1021 gatctccacc gtcttgccgt tgtagcagcg cacgatgtca cccggccggt aggagttgcc
     1081 gtccagcagg ttctcggcga gcggcacgta gaccgacagg cggaccggca gctccaggtc
     1141 ggcgatggcg cgggcggcgc cgaggaccga gcaggcgccg ctcttgtcga acttcatctc
     1201 gtccatgtcg gcggccggct tgatggagat gccgccggag tcgaaggtga cccccttgcc
     1261 gaccagggcg acgcgcgggc cgccggtccc ccaggtcagg cggacgagcc gcggcggcgt
     1321 gctcgacgcg ctgcccacgg ccaggaggcc ccccatgccg cggcgggcga gctcttcctg
     1381 gtccaggacc gtcacctcga agccgcgctc ggcgcccagt tcgcgcgccc gctcctccat
     1441 ccacgcggga ttggcctcgt tcggcggcga gttggcgagg tcgcgcgaat aggcgaccgc
     1501 ctcggcgagc ggcagcgagg tggcgagggc ggactggaag gccccctcct gcccggcggg
     1561 cgggatcacg gcgatccgct ccggccgctc gcgctcgcgc gcctgattga gataacggtc
     1621 atatcggtag ccggtcagcg ccagcgtgcg ggtgatgcgc tcggccgctt cgccgccggt
     1681 ggtctcggca tgcgccggca agaggaaagc cagccggcgc gcgccggtca ggcgagaaat
     1741 ctccggggcc tggaacagcc agcgggccag cttggcccag gtcagctccc ggcgggctcc
     1801 caggccattg agcgagatca ccatgccacc cgggccggtc tccgtctggg ccgcctgctc
     1861 ctcccggccc cgccagccgc tgcgtcccgc cagcctctcc accgccccgc ggaccccttc
     1921 ctcgagggcg tccacttcgg gggcctctcc ctcgaagcag ccaaccagca ggagatccgc
     1981 ttccaggctc tccggcgggg cgacagtcac cgatgcaatt cgcgtcattg ggtcctctct
     2041 caaaagattc cggcggcaga gtgtagcagc cgtgacggcc cgcaaacacc caccctggtc
     2101 acgggaagag ggaacgcggg cgcggcgctt tccctcactc ttgccgtctc cccggtccgg
     2161 atgtgcggga gccgggggcg atatggtata cgtaacgcct tcaattcaag tgcgagaaca
     2221 ggcttccggc gagcatgaac ccgacgtccg tcctgcagct ccttcagggg cctttcaact
     2281 tcctgcgcca ggagccggag gagatcctcc ggctgatgcc cgacctgcag aaggccctgc
     2341 ccacaccgcg gccgatctac ctggaagacg agaccgccaa gccgctcctc ggcttccccg
     2401 tgctcctctc cgaggggatg gaggcgctgc gccaggcgct gcgccagtac atcgccgccg
     2461 aggaggcggt gcaggtggcg atggtgcgcc gcgacacggc cttcgaccgc aaggcctaca
     2521 acgccgcctg ggagcgctac cgctccctgc tgtcgcgggc ggtcgagaac gccaccacct
     2581 cgagctatgg ccggcagtac ccggcgatct tctggctcca tcactctcta gaggtgtctc
     2641 ggaccctcaa ggacacgccc aagcgcatcc ttcggctcga caccgagatc ggccgccgcc
     2701 agggcgacgc catcaaatac cgggtctacg accgcttcct cgaccgcgtc ctcgccacca
     2761 cctatgaggt ggtgcagaag gtcgccggcg ccaccgagga ggtcgaggag gagctcttcc
     2821 cccgtctcct cacccgcatg cgcgacaacg tcctcatctt caccgaggac tacatcggcc
     2881 gcgacctcgc cgagctgggc agctacttcg ccggctcgct gcgcatcgac ggccgcgacc
     2941 tgcggcagcg gctggaggag ctggtgcgct ggcactccga gcagctcgcc gccgacaacg
     3001 agctgcgcag cctggccatc cacgtcgccg gcatggaccc caccaaggag gcccgcgacc
     3061 tgctgaaccg ccccggctgg gtcaccttcc tctccggccg caagggatac aacgccgcga
     3121 agctgctgtc ccccccactg gttcaggtgt gggagagcct gctgatcaag ctcaaggagt
     3181 tcgagctgat gcatggcctg cggcgcgccc tggtcaccgt ggagatgcgc ggcggcgcca
     3241 tcgacctcga cggcacgttc ggcggcatcc gcatggcccc catggtcgcc cgcgacattc
     3301 gctcctcggc cggcggcgag gtgcgcctct cctcggccac ccggccgctc gacttcatgg
     3361 aggcctgggt ggtcgacccg cgggtcgagc gcttcggcat gatctacgac atcagcgact
     3421 tctcgcagac gctgtcgatg ctgcaccgcg gcggcaacga gagccaggac gaggcgttcc
     3481 ggcgcatgtt ccggttccag cggcggatca accggctcgc ccactcgcac cgcgccaagc
     3541 tcgagaagta cctgggggac ggcgccttct actccagccg cgaggcgacg aacctgctgg
     3601 tctgctcggt ccacctgcag cgttactacg agcaggcggt gcgcgagggg ctccccttcg
     3661 accgcggcat gcgcatcgcg ctcaactacg gttcctaccg catgattccg atgggtgccc
     3721 gccccgagga gggggagcgc tacgagttct tcggcccggg cctggtggag ctgtcgcgcc
     3781 tgaccaccgg caaggcgacg caggagatcg acgaggtcaa gaccatgctc gtcagccagg
     3841 gctaccccga ggcgacggtg caccgcttct tcgcgccgct ggcgtcgcgc aacgtcgacg
     3901 tggtggacaa gcgggaggag gcgcggcgct tctattgcta catcaaccgc aacggcaccc
     3961 tgcacaacaa cggcatggtg gggaccggcc ccttcgtcgc cgagctcgag aaggagctgc
     4021 ggtccccccg tctgcgccgc ggcaccgacg gcgaccgcat ctacgtcctc ctcgaggtgg
     4081 agcacgccgg caccccgatg accgtgggct tccgcaagct gggcttcgcg cacctcaagg
     4141 gtctggagaa gctggccgtc tacgagatcg tggacggcgc gacgatcgac cccgcgtcgt
     4201 acgccgacct cccgggagcg agcctctccg ccgccatcga gcgagagttc tccagcacgg
     4261 gcaggacgcc gggggcctcc caggacagcg gggtcagccc ggggctggcg tactaggccc
     4321 tcacccccca ggtcttaata gatagaggtg gtcccagata gaggtggtcc cagggaggtg
     4381 gtcccaggga ggtggtccca gagggaggtg gtcccagagg gaggtggtcc cagggaggtg
     4441 gtgcctcccc ccctgggagg gtgggcggta gggagtgggg gaggtcagga ggggggtttt
     4501 cttcttcctc ttcttcctct tcttcttctt cctcctcttc ttctttttct tctatctctt
     4561 tcttcttccc ttccctcccc ttccctcccc ttccctaccg tccccacctg ttcaactccc
     4621 cgccgatcac ctccaagacc ccctccacgt tcccgaggac ctcatgattc cagaaccgta
     4681 gaacgaggaa cccttcctcc cgaaggaact gatcccgcac cgcatcgctc acgctcccca
     4741 tatgctgacc gccgtcgacc tcaatgacga gcttccgctc gaagcagacg aagtccacga
     4801 tgtactttcc aatcggagct tggcggcgga agcggttgtt caggaaacca cggcgaagat
     4861 aggcccatag atgacgctcc gcgtcggtca tccgctggcg cagttggcgg gcacggtctg
     4921 tgggattcat ggcgcgtccg caggatacgc gcgggggtga tccagaggaa gaagaaaaaa
     4981 agaagaaaac ccccctcctg acctccccca ctccctaccg cccaccctcc caggggggga
     5041 ggcaccacct cccccgcggc tcccgtcgtt ttcgtctggg ccgcctcgat gatcccacca
     5101 tacagatcag ggcaattccg agacgtggga gctggcggat ttccttgcca gcctcgactg
     5161 gaggtcgcaa tcccaccata cagatcaggg caattccgag ggctaccacc aagcctgctg
     5221 acgggccagt ggattaccaa agtcgcaatc ccaccataca gatcagggca attccgaggg
     5281 atcatcaagc gtcaaaaggt tagcgatgcg gaaaagcagt cgcaatccca ccatacagat
     5341 cagggcaatt ccgagttgag tcattaccaa tttcggtccc acgtcgactg gaccttaagt
     5401 cgcaatccca ccatacagat cagggcaatt ccgaggaacg gtgtagcggc ccgcgagcca
     5461 catcgcggag tgcggtcgca atcccaccat acagatcagg gcaattccga gctgccgcat
     5521 gcggcccgcg agcgccgcca gctcggtccg cgtcgcaatc ccaccataca gatcagggca
     5581 attccgagca cataaccgtt tgtaacacat cttacgaagg agaaaggtcg caatcccacc
     5641 atacagatca gggcaattcc gaggaggacg cgatctgggc ggccatcgcc gagcgcttcg
     5701 tcgcaatccc accatacaga tcagggcaat tccgagcccg ggccgat
//
LOCUS       DASPJI010000196         8211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_11473, whole genome shotgun sequence.
ACCESSION   DASPJI010000196 DASPJI010000000
VERSION     DASPJI010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8211)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8211
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11473"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2011
                     /locus_tag="VEL74_05145"
     CDS             <1..2011
                     /locus_tag="VEL74_05145"
                     /inference="COORDINATES: protein motif:HMM:NF020926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HYG61943.1"
                     /translation="WQRSGRVGRAGRESLTAMVALPDALDQYFLDHPDQFFERPCERL
                     VVDPANDPVSRSHLICAAAEMPLSREEDRPYLERHGPRVEKLLDQGHLLEAMESGELF
                     SLRRLPQRTVNLRGTGDQFAILDEGRKGAMIGTIDGVRVLHECHPGAVYLHAGRQYLI
                     SALEREERRVRAEAAELDYFTSPLTEKETEVLEVLEERTDGPLRSWLGRLKVTERVVG
                     FERKRIGSQEVIDQRPLELPPVEFETVGLFWAAPRAIEETLRREGEHFMGSLHASEHA
                     AISLFPLLALCDRGDIGGISYAIHPQLGCGAVFVYDGHPGGVGIAAKGYEDLPGLLRR
                     VQLLVEGCTCEVGCPSCVQSPKCGNGNRPLDRAGAARVLRLLLGFEEPAVAWGEPAVL
                     TLAGPHPPAPSPASPAPSPGEGEASREHDLLAEGPSPLGGGGLDQGGGQEGGVPDRAE
                     PQAFVQEVPLSGRVAVTTRGPIQSFLIQLDRRPANGQELRPPCTVLFDLETIRSATDV
                     GGWGNAHRMGVAIGVACILEEGRFEVYGEGQVRSLAETLRAATLVVGFNIKRFDYKVL
                     AGYTGEDYCRTLPTLDLLESVNSRLGFRVGMGHLAQETLGTAKSADGLQSLAWVKQGR
                     LDLVESYCRKDVEILRDLYLHGRREGCIFYRDRRRDVRLRLCVDW"
     gene            2064..2591
                     /locus_tag="VEL74_05150"
     CDS             2064..2591
                     /locus_tag="VEL74_05150"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HYG61944.1"
                     /translation="MKHTDKYRLLRGLALVAIPLSLLAVLTACSGYGFGQALRSPDVH
                     VVGASVEEVNLAGADLLFEFEVDNPNGLALVLDQVGYKLRINGQHLLDGYQDDRTTIA
                     ARTESRVELPVKIRLDDAYRVLRSFQGRGSDRPQYELEADFRFDAPVVGGITVPVRKT
                     GVIPVERLLQYIPSR"
     gene            2736..4745
                     /locus_tag="VEL74_05155"
     CDS             2736..4745
                     /locus_tag="VEL74_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYG61945.1"
                     /translation="MRPILVSVSALVLMTSMLAGAAPQGAERQYTFLLAGNRAGTMTV
                     REAGEGGRQVEASFEFNDRGRGPKVTERAVFGASGLPERVEIEGIDYWKKPVTETFQN
                     TGGKASWSNGAEKGSGAGKAAFYLGLNGVPEETARLAAALLAAPEGRLELLPAGEARI
                     ERAGSTEVKTAKESRKVDLYAISGLGFEPTFVWLDADRRLFASYSGWATTIRAGWEDV
                     VPRIAEVQDAAAATREKDLAARLARRPAQGLVVKGARLFDPATGKVAPAMTVVVSGNK
                     VQAVGKDGEVAAPAGAEVIDARGRMLLPGLWDMHAHLGTVDGILNLAAGVTTVRDMAN
                     ETDQLLALRDRFDKGEALGPRVILAGFMDGPGPFAGPTKVLISKVDEALAAVDRYAKL
                     GYSQVKLYSSLDPALVAPIVERAHRHGLRVSGHIPNGMSAEQAVRAGFDEIQHTNFLF
                     LNFLSGVDTRTPARFTEVAARGAELDLGSERVRAFLRLLQERGVTVDPTLATFEGMFT
                     GRSGEIDPAFATVADRLPSQVRRGFLSGGLQAPEGMEQRYRDFYRAMIQMVGALHKAG
                     VTLVAGTDATPGFALHRELELYADAGIPAPEILRIATLGAAKVMKRDQDLGTVAPGKL
                     ADFILVDGDPTARIADIRRVVLTVKDGAVYDPAALYTAIGVKPAV"
     gene            4955..5329
                     /locus_tag="VEL74_05160"
     CDS             4955..5329
                     /locus_tag="VEL74_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61946.1"
                     /translation="MSPKVRSLVTLALLALLVAGPAAALPVGGRTPSMEGASVFSGVW
                     DWFSSLVSGLWGKAGSSMDPDGPPASNKAGSSMDPNGAPTPGEEGGSMDPDGLNATGC
                     TTEAGGTMDPNGKPIPCPAPAG"
     gene            5652..7007
                     /locus_tag="VEL74_05165"
     CDS             5652..7007
                     /locus_tag="VEL74_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61947.1"
                     /translation="MKIHPNDLFLEEFLLAMSPEYRMLVLHLCRCTSCRRRVRRLLTE
                     PPAPAAQRLARVLLGSQSWNGHEQTSAQEPPFEALGFALAKERAEAPGLFVELTSHES
                     EQRTLLLENSNRFHTWGLYELLVERSLESAIRNPAYAEDLGFLALKVSEQLDTSCYGE
                     EVIEDLRARSWAHIGNARRIRSDLHGAEEAFQLAYGHLQSGTQEPLERALFLDLRASL
                     RKDQRKFGEAFSLLQRAVALFLEIGQQHRAGKSLVSMSTVHEQEGHPEEGIPLLYQAL
                     ELLDAEQEPRMLLCARHNLIFCLSETERFVEAQSLYRQTRELYREFPERWVQNRRKWV
                     RGKIARGLGQTHHAEVLFLAARDGFIAEGVPYDTALVSLEIASLYAEQGRLAELKRLA
                     EEMLPIFTSRQIHREAMAAVAFFLHAAEAEQVSVGLVSSVADFLRRAQHDPEQRFQPP
                     A"
     gene            complement(7611..>8211)
                     /locus_tag="VEL74_05170"
     CDS             complement(7611..>8211)
                     /locus_tag="VEL74_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYG61948.1"
                     /translation="RDFGVLTPEDPYPVWPGEGPRCILAPLFLLYDYSFRPDDVPEDQ
                     AISWAAEDNLLCSDEVLLHSDPYPTKTAWCHARCDEAEQRLTEAAARGPLVLINHFPL
                     RRDLAILPRIPRFSIWCGTRRPEDWHLRFRAEVVIYGHLHIRGTHHRDGVRFEEVSLG
                     YPQNWEPRRGIEPYLRQILPHPHKPPQSPGGPSHVLAEG"
BASE COUNT         1245 a         2798 c         2831 g         1337 t
ORIGIN      
        1 ctggcagcgc tcggggcggg tggggcgggc cggccgcgag tccctcaccg cgatggtcgc
       61 gctgcccgac gccctcgacc agtacttcct cgatcacccc gaccagttct tcgagcgccc
      121 ctgcgagcgg ctggtggtgg acccggcgaa cgacccggtc tcccgctcgc acctgatctg
      181 cgccgcggcc gagatgccgc tctcccggga ggaggaccgg ccctacctcg agcggcacgg
      241 cccgcgggtg gagaagctcc tcgaccaggg gcacctcctc gaggccatgg agtcgggcga
      301 gctcttctcg ctccgccgcc tgccgcagcg caccgtcaac ctccgcggca cgggcgacca
      361 gttcgccatc ctcgacgagg gccgcaaggg ggcgatgatc ggcaccatcg acggcgtgcg
      421 ggtgctccac gagtgccacc ccggggcggt ctacctccac gccggacggc aatacctgat
      481 ctcggccctg gagcgcgagg agcggcgggt gcgggccgag gcggccgagc tcgactactt
      541 cacgtcgccg ctgaccgaga aggagaccga ggtcctggag gtgctggagg agcgcaccga
      601 tgggccgttg cgctcctggc tggggcggct caaggtcacc gagcgggtgg tgggcttcga
      661 acgcaagcgg atcggcagcc aggaggtcat cgaccagcgg cctctcgagc tcccgccggt
      721 cgagttcgag accgtggggc tgttctgggc ggcgccgcgg gcaatcgagg agacgctgcg
      781 aagggagggg gagcatttca tgggatcgct ccacgcctcc gagcacgccg ccatctccct
      841 cttcccgctg ctcgccctct gcgaccgcgg ggatatcggc gggatctcct acgcgatcca
      901 ccctcaattg ggatgtggcg cggtgttcgt ctacgacggc catcccggcg gggtggggat
      961 cgcggccaag ggctacgaag acctgccggg gctcctgcgg cgggtgcagt tgctggtgga
     1021 ggggtgcacg tgcgaggtcg gatgcccctc ctgtgtccag tccccgaagt gcggcaacgg
     1081 caaccgtccc ctggaccgtg ccggcgccgc ccgcgtgctc cggctgctgc tcggcttcga
     1141 ggagccggcg gtggcctggg gcgagccggc ggtgctgacg ctggcgggcc ctcacccccc
     1201 ggccccctct cccgcctccc cggccccctc cccgggagag ggggaggctt ccagggagca
     1261 cgacctgttg gcggagggtc cctcccccct gggagggggc gggttggatc aggggggagg
     1321 acaggagggg ggcgttccgg atcgcgccga acctcaggcc ttcgtccagg aggttcctct
     1381 ctccggccgc gtcgccgtca ccacccgcgg gccgatccag agcttcctga tccagctcga
     1441 ccggaggccg gccaacggac aggagctccg gcccccctgc acggtgctct tcgacctgga
     1501 gacgatccgc tcggcgaccg acgtgggcgg ctgggggaac gcgcaccgga tgggggtcgc
     1561 catcggggtg gcctgcatcc tggaagaggg gcgcttcgaa gtctatggag aaggacaggt
     1621 gcgctctctg gcggagacgc tccgggccgc caccctggtc gtgggcttca acatcaagcg
     1681 cttcgactac aaggtcctgg cgggctacac gggcgaggac tactgccgga ccctgcccac
     1741 cctcgacctc ctggagtcgg tgaactcccg gctcggcttc cgggtcggga tggggcacct
     1801 cgcccaggag acgctgggga ccgccaagtc cgccgacggc ctccagagcc tcgcctgggt
     1861 gaagcagggg aggctcgacc tcgtcgagag ctactgccgc aaggacgtgg agatcctccg
     1921 cgacctctat ctccatggcc gccgcgaagg gtgcatcttc tatcgcgatc gcaggcgtga
     1981 cgtccgcctc aggctctgcg tggactggta ggcggaccct atggcacagc ctttgccttg
     2041 accaggaagc ggaggacttc gccatgaagc acaccgataa atatcgtctt ctccgcgggt
     2101 tggccctcgt cgccatcccg ctgtcccttc tcgccgtcct gaccgcttgc tccggctacg
     2161 gcttcgggca ggccctgcgc agcccggacg tccacgtcgt cggcgccagc gtcgaggagg
     2221 tgaacctcgc cggcgcggat ctgctcttcg agttcgaggt ggacaacccc aacggcctgg
     2281 cgctggtgct ggaccaggtg ggctacaagt tgaggatcaa cggccagcac ctgctggacg
     2341 gctatcagga cgaccgcacg accatcgccg cccggaccga gagccgcgtc gagctgccgg
     2401 tcaagatccg gctcgacgac gcctatcgcg tgctccgcag cttccagggg cgggggagcg
     2461 accggccgca gtacgagctg gaggccgact tcaggttcga cgcgccggtg gtgggcggca
     2521 tcaccgtgcc ggtacgcaag accggggtga tcccggtgga gcggctgctc cagtacatcc
     2581 cgtcccggta ggcgagctgc ctccggtcct tgggagggag gggccctgct cggctccttc
     2641 gtgtgcgctc cttcctcccc ccacccacgc tgccagtcgc ctgcgccggg cccctccctc
     2701 ccaaggacct cccccctccc tgtatgatgc gggccatgcg cccgatcctc gtctccgtct
     2761 ccgcccttgt cctgatgacc tcgatgctgg ctggcgctgc ccctcagggc gccgaacgcc
     2821 agtacacctt tcttttggcc ggcaaccgcg cggggacgat gaccgtgcgc gaagccggag
     2881 aggggggccg tcaggtcgag gcctcgttcg agttcaacga ccggggccgc ggcccgaagg
     2941 tgacggagcg cgcggtgttc ggggcctccg gtctgcccga gcgggtggag atcgagggca
     3001 tcgactactg gaagaagccg gtgacggaga cgttccagaa caccggcggc aaggcttcct
     3061 ggagcaacgg cgcggagaag gggagcgggg ccgggaaggc cgccttctat ctggggctga
     3121 acggcgtccc ggaggagacg gcgcgcctgg ccgcggccct gctcgccgct ccggaagggc
     3181 ggctcgagct cctgccggca ggcgaggccc ggatcgagcg ggcggggtcc accgaggtga
     3241 agacggcgaa ggagtcccgc aaggtggacc tctatgccat ctccgggctc ggcttcgagc
     3301 cgacgttcgt ctggctcgac gccgaccggc gcctcttcgc ctcctattcg gggtgggcga
     3361 ccaccatccg cgccggctgg gaggacgtcg tgccgcggat cgcggaggtc caggacgccg
     3421 cggccgccac ccgcgagaag gatctggcgg cccggctcgc gcgccggccg gctcagggcc
     3481 tggtggtgaa gggggcccgt ctgttcgatc ccgcgacggg caaggtcgcc cccgccatga
     3541 ccgtggtcgt ctcgggcaac aaggtgcagg cggtgggcaa ggacggcgag gtggcggcgc
     3601 cggccggggc cgaggtgatc gacgcccgcg ggcggatgct cctgcccggc ctgtgggaca
     3661 tgcacgccca tctcgggacg gtggacggca tcctgaacct ggcggccggg gtcaccaccg
     3721 tccgcgacat ggccaacgag accgaccagc tcctcgccct gagggaccgc ttcgacaagg
     3781 gcgaagcgct ggggccgcgg gtgatcctgg ccgggttcat ggacggcccg ggccccttcg
     3841 cgggccctac aaaggtgctg atctcgaagg tggacgaggc cctcgccgcc gtggaccgct
     3901 acgccaagct cggctactcg caggtcaagc tctacagctc gctcgatccg gccctggtgg
     3961 cgcccatcgt ggagcgggcg caccggcacg ggctgcgcgt ctccggccac atccccaacg
     4021 gcatgagcgc cgagcaggcg gtgcgcgcgg gtttcgacga gatccagcac accaacttcc
     4081 tgttcctcaa cttcctcagc ggagtcgaca cccggacgcc ggcgcgcttc accgaagtgg
     4141 cggcgcgggg ggcggagctg gacctgggct cggagcgggt gcgggccttc ctgcggctgc
     4201 tccaggagcg cggggtgacc gtggacccga cgctcgccac cttcgagggg atgttcaccg
     4261 gccgttccgg ggaaatcgac cccgccttcg ccacggtggc cgaccgcctg ccgtcgcagg
     4321 tccgccgggg tttcctgtcc ggcggcctgc aggcgcccga ggggatggag cagcgctacc
     4381 gcgactttta ccgggcgatg atccagatgg tgggcgccct ccacaaggcc ggggtgaccc
     4441 tcgtggccgg caccgacgcg acgcccggct tcgcgctcca ccgcgagctg gagctctatg
     4501 ccgacgcggg catccccgcg ccggagatcc tccgcatcgc caccctggga gccgccaagg
     4561 tgatgaagcg ggaccaggac ctcggcaccg tcgcgcccgg caagctcgcc gacttcatcc
     4621 tggtggacgg cgatcccacg gcccggatcg ccgacatccg ccgcgtcgtc ctcaccgtca
     4681 aggacggcgc cgtctacgat cccgccgcgc tctacacggc gatcggcgtg aagcccgccg
     4741 tctgacggcg ccggctcgtc accgccggcc ggaaccgcgt ttccttcttc aggacatcct
     4801 tcaggaaagg tttcctccct gcttcaggga ggaacggagg cgctttctct tccaccggcg
     4861 ctgctctcgg gaaaaaatga cactctcgcg gtaggcaagc cccccagcgg ccatggtggc
     4921 tccatgggag aaggtttgtc aaaaggagat tgtcatgtcg cccaaggttc gctctctcgt
     4981 caccctcgcc ctcctggccc tcctcgtcgc cggtcccgct gccgctcttc ccgtaggagg
     5041 ccgtactcct tccatggagg gggcctcggt cttttccggg gtctgggact ggttctcgtc
     5101 cctggtcagc gggctttggg ggaaggccgg gagcagcatg gatcccgacg gcccccccgc
     5161 ctccaacaag gccgggagca gcatggaccc caacggcgcc cccacccccg gcgaggaggg
     5221 gggctcaatg gatcccgatg gcctgaacgc caccgggtgc accaccgagg ccgggggcac
     5281 catggaccct aacggcaagc cgatcccctg tccggcaccg gccgggtaag gcagggccaa
     5341 ggcaaaggcg ccggtgtgcc gccggcgcct ctttcgcctc ggagggctac ttttacgctc
     5401 tttcccaagg cgtctctttc gacgaaaccg acaagcaggc gaggcccgca tagtcagacc
     5461 tgaagcgcat acttgtaggc tattgacgcc ggtatgctgc ggacaccatc ttttcctgaa
     5521 gtcgctgtcc cgcttaccgc ggccacggcg actaatgttc ttaggacaca ctgcccgtgc
     5581 ctcctgtagg agagattaaa atcctgtcgt tttcgcgcta gaccctgtga actctagaga
     5641 ggagggagct gttgaagatc cacccgaatg atctgttcct ggaggaattc ctcttggcca
     5701 tgagccccga gtaccggatg ctcgtcctgc acctttgccg gtgtacgagt tgccgccggc
     5761 gggtccgccg gctgctgacg gagccgccgg cgcctgctgc ccagaggctc gcccgggtcc
     5821 tgctggggtc ccaaagctgg aatgggcatg agcagacttc ggcccaggaa ccgccgttcg
     5881 aagcgctcgg cttcgcgctc gccaaggagc gggcagaggc gccgggcttg ttcgtcgagt
     5941 tgacgagcca cgagtccgag cagcggactc tgctcctcga aaattcgaac cgcttccaca
     6001 cctggggtct ctatgagctg ctggtcgaga ggagtctaga gtccgctatc cggaaccctg
     6061 cctatgctga ggacttgggg tttcttgccc tcaaggtttc ggagcagttg gataccagct
     6121 gctacggaga ggaagtgatc gaggatctac gggctcgtag ttgggctcac attgggaacg
     6181 cacggcggat ccgttcagat ctgcatggag cggaggaggc cttccaactt gcctatggtc
     6241 acctccagag tggaactcaa gagcctctgg agcgagcgct gtttctggac ctcagagcct
     6301 ccctcaggaa ggatcagcgc aaatttggcg aggcgttcag tctgctccaa cgagctgtcg
     6361 ccctcttttt ggagatcggg cagcagcacc gggcagggaa gtcgctggtg agcatgtcga
     6421 cagttcatga gcaagagggt catcctgagg aaggtattcc tctcctatac caggctctgg
     6481 aactcctcga cgctgagcag gagccccgga tgctcctctg cgctcgtcac aacttgatct
     6541 tctgtctttc agagacagaa cggtttgttg aggcgcagag tctgtatcgt cagactcggg
     6601 agttataccg agagtttccg gaacgctggg tccagaaccg ccgcaagtgg gtgcgcggca
     6661 agatcgcccg cgggctgggc cagacgcatc acgccgaggt gctcttcctg gcggcccgcg
     6721 atggcttcat cgccgagggg gtcccctacg acacggcttt ggtttcgctg gagattgcct
     6781 cgctctatgc cgagcagggg cgcctcgcag agctcaagcg tctggccgag gagatgctgc
     6841 ccatcttcac ctcgcggcag atccaccgcg aggccatggc cgcggtcgcc ttcttcctgc
     6901 atgcggccga ggcggagcag gtgagcgtcg gcctcgtctc cagcgtcgcc gacttcctgc
     6961 gccgcgccca gcacgatccc gagcagcgct tccagccgcc cgcttgaacc gtcccgctaa
     7021 gcttggcggg ctcccgatgg agtctccgcc aagcttaccc ctccccgccg ggatttcttc
     7081 gtcgcagagc agcgttcgtt cgtcgcagat ctgcaccact ctgcgacgaa cgaagagagc
     7141 tctgcgacgc aggagcgttc gtctgcgacg tttgaccagg gctctgcgat gaacgaacgc
     7201 agctctgcga cgtttgagcg cagctctgcg atgaatgagt gcagctctgc gatgtttgag
     7261 cgcagctctg cgacgtttca gggtaactct gcgacgttcg gctcgtcgcc gtcgccttcg
     7321 cgcaagctgt acgactcgcg ccgaggggtg gcggcgtcct ccctccctgg gcgggggcgt
     7381 ggtagggagt gggggaggac cgaggagggg ggttgctttt ggggcgggcg gcggagatcc
     7441 tctcggatcc gcggaaaaga agctgccccc ctcctgacct cccccactcc ctaccgccca
     7501 cccacccagg gggggaggca ccaccgccca ctcgcaccta caagttaacg cctatgggag
     7561 gacacactac acccttgcgg ccccaagtta gcgcgtatgg gccaccgccc tcacccttcg
     7621 gcgagcacgt gcgacgggcc gcccggcgac tgcggcggtt tgtgcggatg agggaggatc
     7681 tggcgcaggt agggctcgat cccccggcgc ggctcccagt tctggggata gcccagcgac
     7741 acctcctcga agcgcacgcc gtcccggtgg tgcgtgccgc ggatgtggag gtggccgtag
     7801 atcaccacct cggcgcggaa gcggaggtgc cagtcctcgg gccggcgggt gccgcaccag
     7861 atggagaagc gcgggatgcg ggggaggatc gccaggtccc gccgcaacgg gaagtggttg
     7921 atcagcacca gcggcccccg cgccgccgcc tcggtcagac gctgctcggc ctcgtcgcaa
     7981 cgggcgtggc accaggccgt cttcgtgggg taggggtcgg agtggagcag cacctcgtcc
     8041 gagcacagga ggttgtcctc cgccgcccag gagatcgcct ggtcctcggg cacgtcgtcc
     8101 ggacggaagc tgtagtcata gagaaggaag agcggcgcca ggatacagcg cggcccctcc
     8161 cccggccaga ccggatacgg gtcctccggc gtcagcaccc cgaagtcccg g
//
LOCUS       DASPJI010000197         3270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_303441, whole genome shotgun sequence.
ACCESSION   DASPJI010000197 DASPJI010000000
VERSION     DASPJI010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303441"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /gene="tilS"
                     /locus_tag="VEL74_05175"
     CDS             <1..286
                     /gene="tilS"
                     /locus_tag="VEL74_05175"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HYG61949.1"
                     /translation="GSPHRAGLVLPLTPGDHVTIRNRRPGDRLLPLGAGGSRRLKEVL
                     IDRKVPREERDRLPLLCVGERIAWIPGVTIDQRFRITGETTAWVAEVTTQ"
     gene            283..852
                     /locus_tag="VEL74_05180"
     CDS             283..852
                     /locus_tag="VEL74_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HYG61950.1"
                     /translation="MRPKRSPVPKPPKIETLFDADALRTQIAALGRRLEEDLGGEDPM
                     LLALLGGSFVFFADLVRAIERPVRFELIHVGYYATHAQVGQEEGEVLQIQYPIPVDIE
                     GQSVIVVKDVVGSGVTEPYLEQQLRDRGAGAVRFAALIDRPDERKTSYELPYSAFTTN
                     EPGLLVGYGMKHEGRYGNLPYVGRLAGGA"
     gene            958..2982
                     /gene="ftsH"
                     /locus_tag="VEL74_05185"
     CDS             958..2982
                     /gene="ftsH"
                     /locus_tag="VEL74_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HYG61951.1"
                     /translation="MNSTLKTLFLWMAIFVVVILLWNTFQHGKSSEEELNFTAFMEQV
                     TSGRVKEVTIHDGREIRGKLKGGGEYPEGTAFTTQLPFQSDSDFIARLERSNVDVSAD
                     ETKENPLLAVLFTWAPVLLIVALWIFFMRQMQTGGNKALSFGKSKAKLLNTSGKKVTF
                     KDVSGVEEAKEELQEIVEFLKEPQKFQKLGGRIPKGVLLMGPPGTGKTLLARAIAGEA
                     NVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGG
                     HDEREQTLNQLLVEMDGFESNEGVILIAATNRPDVLDPALLRPGRFDRRVVVDRPDIN
                     GRLGILKVHTRNIPLSDDVQLLNIARGTPGFAGADLANLVNEAALIAARRNKKKVDMG
                     DFEYAKDKVIMGVERKTMMLTDEEKLVTAYHEAGHALVAAFTPGSDPLHKVTIIPRGR
                     ALGLTMQLPTEDKYTYRKQYVEAQIAILMGGRVAEELTQPDITTGAGNDIERATDMAR
                     RMVCEWGMSDLGPLAYGTKDEPVFLGREFNQRSDYSEDTAIRIDREVARIVNTAHRKA
                     TGILTEYRRVLDRVAHDLLERESLDGEEVYRTVREMTGRDPAPTRPVPPGEGMVVGGH
                     EVPEQVLTPRNAAPAGDVTTEVVDEEEVEAKVAGAQRRAAQPVPEPENSR"
BASE COUNT          547 a         1167 c         1091 g          465 t
ORIGIN      
        1 cggctcgccc caccgggcag gtctggtgct gcctctgacc cctggtgacc acgtcacgat
       61 ccgcaaccga cgtcccgggg accgcctgtt gccgctgggc gccggcggca gccgccggtt
      121 gaaggaggtg ctcatcgacc gcaaggtgcc gcgtgaggag cgggacaggc ttccgctgct
      181 ctgcgtggga gagcggatcg cgtggatccc gggcgtcacc atcgaccaac gattccggat
      241 caccggagaa acgaccgcct gggtcgccga ggtcacgacg caatgaggcc gaaacgctcg
      301 cccgtcccca aaccgccgaa gatcgaaacc ctcttcgacg ccgacgccct gcgcactcag
      361 atcgccgccc tgggccggcg cctggaggag gatctggggg gcgaggaccc catgctcctc
      421 gccctgctcg gaggctcctt cgtcttcttc gccgacctgg tgcgcgccat cgagcggccg
      481 gtgcgcttcg agctgatcca cgtgggctac tacgccaccc atgcccaggt cggccaggaa
      541 gagggtgagg tgctgcagat ccagtacccc atcccggtgg acatcgaggg gcagagcgtg
      601 atcgtggtca aggacgtggt gggctcgggc gttaccgagc cctatctgga gcagcagctc
      661 cgggaccgcg gcgcgggcgc cgtgcgcttc gccgcgttga tcgaccggcc ggacgagcgc
      721 aagacgtcct acgagctgcc gtacagcgcc ttcaccacca acgagccggg cctcctggtc
      781 ggctatggga tgaagcacga ggggcgctac ggcaatctgc cctatgtggg acgtctcgcc
      841 gggggggctt gaggcgtgcc gggaatcgat gcccccgccc gccgggttgt cactgtgaag
      901 ggaccctacg gtcccatggg gagcgcccct ggcgctccgg agtgaggtag ccgaaccttg
      961 aattcaactc tcaagacgct gtttctctgg atggccatct tcgtcgtggt catcctgctg
     1021 tggaacacct tccagcacgg caagagctcg gaggaggagc tgaacttcac cgccttcatg
     1081 gagcaggtga cgtcgggccg ggtgaaggag gtgacgatcc acgacggccg cgagatccgc
     1141 ggcaagctca agggcggcgg cgagtatccc gaagggaccg cgttcaccac ccagctcccc
     1201 ttccagtcgg actccgactt catcgcccgc ctggagcgtt ccaacgtcga cgtcagcgcc
     1261 gacgagacca aggagaaccc gctgctcgcg gtgctcttca cctgggcgcc ggtgctgctc
     1321 atcgtggcgc tgtggatctt cttcatgcgc cagatgcaga ccgggggcaa caaggcgctg
     1381 tccttcggca agagcaaggc aaagctcctg aacacgtcgg gcaagaaggt gaccttcaag
     1441 gacgtctccg gcgtcgagga ggccaaggag gagctgcagg agatcgtcga gttcctcaag
     1501 gagccccaga aattccagaa gctgggcggc cgcatcccca agggcgtgct gctgatgggc
     1561 cctccgggaa ccggcaagac gctgctcgcc cgcgccatcg ccggcgaggc caacgtcccc
     1621 ttcttctcca tctccggctc ggacttcgtc gagatgttcg tcggcgtcgg cgcgtcccgc
     1681 gtgcgcgacc tgttcgagca gggcaagaag aacgccccct gcatcatctt catcgacgag
     1741 atcgacgccg tcggccgcca tcgcggcgcc ggcctgggcg gcggtcacga cgagcgcgag
     1801 cagacgctca accagctgct ggtggagatg gacggcttcg agtccaacga gggggtcatc
     1861 ctgatcgcgg cgaccaaccg ccccgacgtg ctcgacccgg ccctgctgcg ccccggccgc
     1921 ttcgaccgcc gcgtggtggt ggaccggccg gacatcaacg gccgcctcgg gatcctcaag
     1981 gtccacaccc gcaacatccc gctgtccgac gacgtccagc tcctcaacat cgcccgtggc
     2041 acccccggct tcgccggcgc cgacctggcc aacctggtca acgaggccgc gctcatcgcc
     2101 gcccgccgca acaagaagaa ggtcgacatg ggcgacttcg agtacgccaa ggacaaggtc
     2161 atcatgggcg tcgagcgcaa gaccatgatg ctgaccgacg aggagaagct ggtcaccgcc
     2221 tatcacgagg cggggcacgc cctggtggcg gccttcaccc cgggatcgga cccgctccac
     2281 aaggtcacca tcatcccgcg cggccgggct ctgggcctga ccatgcagct ccccaccgag
     2341 gacaagtaca cgtaccgcaa gcagtacgtc gaggcgcaga tcgccatcct catgggcggc
     2401 cgggtggccg aggagctgac ccagcccgac atcaccaccg gcgccggcaa cgacatcgag
     2461 cgggcgaccg acatggcgcg gcgcatggtg tgcgagtggg gcatgtccga cctcggtccg
     2521 ctggcctacg gcaccaagga cgagccggtc ttcctcggcc gcgagttcaa ccagcgctcc
     2581 gactacagcg aggacaccgc catccgcatc gaccgcgagg tggcccgcat cgtcaacacc
     2641 gcgcaccgca aggccacggg gatcctcacc gagtaccgcc gcgtgctcga ccgggtggcc
     2701 cacgacctgc tggagcgcga gtcgctggac ggcgaggagg tctaccggac cgtccgcgag
     2761 atgacgggcc gcgatcccgc cccgacccga cccgtgcctc cgggcgaggg catggtcgtc
     2821 ggcggccatg aggtgccgga gcaggtgctg acgccccgca acgccgcgcc tgccggcgac
     2881 gtcaccaccg aggtggtgga cgaggaagag gtggaggcga aggtggcggg cgcccagcgg
     2941 cgcgccgccc agccggtgcc cgagcccgag aattcgagat aggacggagc cgcccgtccc
     3001 cggatccctg ccagcgcccg gctccccgcc gggcgctggc gtcttcaggg ggttcgccgc
     3061 cgcctcgcgc tcacgataga cttagcgccc gcgggccccc tgcccgtcca ccgcatggag
     3121 atcgcccatc ccccccacac cgtccgcgcc atccgcgccg tccgcagatg gaccctccgg
     3181 ctcccccgcg ggcggactct cgtgctggga gagcggccgc tggtgatggg cgtgctgaac
     3241 ctcacccccg actccttcag cgacggcggc
//
LOCUS       DASPJI010000198        12603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_72055, whole genome shotgun sequence.
ACCESSION   DASPJI010000198 DASPJI010000000
VERSION     DASPJI010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12603)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12603
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72055"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..2062
                     /locus_tag="VEL74_05190"
     CDS             167..2062
                     /locus_tag="VEL74_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYG61952.1"
                     /translation="MADELAEIAGDAPAEETPEEDTGLGIADELAALDEAPAEDQDAE
                     GDRVRQAVAAFAKAHPEVQAVRVVRFDGILLEASTASEDTGERAAPRRLDRDEKPLYD
                     QGQSLRAAIEGNREEGAARAPEIAVERRAGGVLRLSAPFETGDEVVGMVQMDTRPLQS
                     AASFPWLPALLTWLAPVVLFLLLSLVLGERRTLLAAVAVILLLAALLGFGRWARTALE
                     QERRATGAAVASTVTAEAERAEALLAELGLPAAEPLAPELWDVDLYRQPLGLVQPGGT
                     LDEGRLGEFAGADAGRLTRSVIVLALAALAVLAFIGLGFAARTGAALVRYRNAYAYTL
                     PALIAMLVLVFFPFFYGIALAFTNANVYNTGQSIFETWIGLENFVNILSDLGVVERTA
                     QGLVIDYTNFYWTLGFTIVWTITNVAIGVSLGLLLALALNTKGLAFRPIYRVLLILPW
                     AVPTYITALIWQGMFHRQFGVINQIIQMVGGNPIAWFDAPLTSFLAVLTTNGWLSFPF
                     MMVVSLGALQSIPADLYEAARVDGASRWQQFRSITLPSLRPALIPAIILSVIWTFNQF
                     NVIYLVSAGQPAGATEILITKAYKLAFENYRYGYAAAYSTVIFLILLVYGTWQNRVTR
                     ATEAVGE"
     gene            2067..2948
                     /locus_tag="VEL74_05195"
     CDS             2067..2948
                     /locus_tag="VEL74_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HYG61953.1"
                     /translation="MSRKRSQEISHLPLHLFLLLMVAVTVYPILWVITIAFSGGQSLA
                     IADIPQDPTLADRVRAVTPWPEQFSMSNFTSLWADQPFLRWLFNSAVVAGLTTLVGVF
                     LSSTAAYAFSRFRFPGRRAGLMSFLVSQMFPGTLMLIPLYIIVVQWLGLGSSFTGLVL
                     VYSTIAIPFCVWMMKGYFDTIPKELEESALIDGASQAMIFFRIVLPLAKPAVAVTALF
                     SFMTGWNEFIIAATFMNREEMYTGPVGLRFFVGGFSQQWGYFAAGSIIAAIPVVLLFL
                     FLQKYLVSGLTAGAVKG"
     gene            2974..3798
                     /locus_tag="VEL74_05200"
     CDS             2974..3798
                     /locus_tag="VEL74_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HYG61954.1"
                     /translation="MRSTHRKTFSALSAFGAGLLVAASLSAQSVSFKDPANDDNGPGT
                     YTYPTDGVYKRGSFDLTGLDIEAKGNKVDFGVSFNTPLEDPWRMGTGFSVEMVFIFID
                     NKEGGMKESFPGINVNFAEGSEWDKLVILSPQPPGRVKTEVKEKLPAAMQAAVIVPSR
                     VKGAGRTISATVGLDELGGGDPSTWGYQVVVQSNEGFPEGKDLMTRKVNEYEGQHRFG
                     GGSDYDCDPHAIDVLAGNGTGAADEAAAQHAMLKYECGPDGAVKTAATLKMVRKGS"
     gene            3837..6044
                     /locus_tag="VEL74_05205"
     CDS             3837..6044
                     /locus_tag="VEL74_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61955.1"
                     /translation="MRFQRGFAFILLATAALASSVTPAHAADGQTLLITGEGYIKWLW
                     GNQRTDGSLYNFTTIPNEGYGDNGQGTEINLLLASRPSRNTEVTGRIQSRFSQNQWTN
                     FGGFGGRNPAFEEPPGAPCVGGDCGEFDPRSNEYIKMRGLTARYTPGWKLVDAITVGS
                     TDLGMFDPWTIGKIRYIDRDNAKAVLLQGSLANNFGYDLIRVSLPRLWAGPDFNTGSY
                     TAADGAYGLQLRATPSPLFDFVGVVETVSDLEVDTLDFDYDDGRDVEARFSNDVYGVR
                     FGIHPSTLIDIRGMAYFSQAESDPRFGVPSSFGLSGFSPVPAGEIDDEAFKANVDLND
                     PFGVGLSFNLEYFDIGAQYISILAARREADVLLTEGSDGAFVFPGPDNSAFGVFGGNP
                     TRIGYGGFQGNAQQVATINVDNEFTDFDEAMAESVIGWKGFTIAPNWSLGGFDLSAEY
                     SFIDYNTNWQAWGDPSRGIESSIYPNFESDAGVNSYRNAYAPFQEKETTIMVLRGKYV
                     LDVAKGVDLFGKVKLIDEQDDRLDDPRFLPYQAGDCPGGGAACSNNARDYSPGNSTAA
                     IYGNPPVITGAGGVVGYQWAPFDDISDDDRDMDYRMIQLGGGYQFTDNLYSSLTFERY
                     DVDLLDGNTAFQAYRLHSMSSGEHTKNKVILFARYTLGGSEFGFNYEYNFGEFDPDFG
                     GGFVPQVISDDTARSLGLPFGSLGFAGRFGGWNSLETREFRQNRLKAFLKFRF"
     gene            6122..7096
                     /locus_tag="VEL74_05210"
     CDS             6122..7096
                     /locus_tag="VEL74_05210"
                     /inference="COORDINATES: protein motif:HMM:NF021500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HYG61956.1"
                     /translation="MHSSRTPVLAFTLAAALLAAPAFGRSEIFTLNDPRGDDHGDGNY
                     VYPLNDELNEGDLDLLSLTARREGAGTQFELTFARPVKTPGRVAIDYSGTQLDTVARF
                     GFYTLNVDLYIDMDRAPGSGGTSMLPGRLAEIDPASAWERAVVLTPRPHEARGELKRM
                     LARQLRQDARDEDSDLGQAQLEALQKQIPGDVESRIFFPTQVRVRGSKISFFVPDSFL
                     GGPAKDTWSYVVAVSAADLLQSFDVNRALGRQSEDEEALMILPVSPGKWQDRIGGGRE
                     GAPLQPPLMDIIAPEGRKQETILGDFDSRARRPAVLPGVVPAEQPKKK"
     gene            complement(7099..8808)
                     /locus_tag="VEL74_05215"
     CDS             complement(7099..8808)
                     /locus_tag="VEL74_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HYG61957.1"
                     /translation="MRTLPTLLAPLATLLALLPACAGGSAAPPAPPTPPAVGALASPE
                     APPAKGPVQWQHDWARGAVFYEVFVRSFADSDGDGIGDIRGLTSKLDYLNDGNPKTVT
                     DLGVDALWLMPVFESPSYHGYDVVDYERIDREYGTAEDFQRFLDEAHRRGIRVIVDFV
                     MNHSSSQHPWFVESASSPASGRRDWYVWRADDPRWTQPWGGNNRTWHEKNGAYYYGVF
                     WGGMPDLNFSTPAVREEMKRLATLWLGRGLDGFRLDATRHLFANGPGAGQNDQPETHV
                     YLKELSAAVRQAKPDAMLVGENWTETPEIAAYYGSTDTVRGGDELPMNFNFPLASAIL
                     AGVNAGDAQPIAEKLTEMAELYPAGIIDTPFLTNHDMVRAATQLGADPGERVARSKMA
                     AAVLLTLPGAPFLYYGEEVGLQNGGPSSSDELKRTPMPWDASPGGGFTTGKPWYDFAP
                     GRETNNVAAQAGDPDSLLTHYRHLIRARHNSDALRTGELELRTPATTNSPLLAFLRKS
                     GKETVLVVHNLGSEPVQAGPYEVSGRPDPLFASAGVGRPAQAAGGWTFQLPGRTSGVW
                     VLR"
     gene            8864..9568
                     /locus_tag="VEL74_05220"
     CDS             8864..9568
                     /locus_tag="VEL74_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010479728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYG61958.1"
                     /translation="MDTPQRVAVVTGASRGLGAAFARVLSEEGFAVALGARDVDAVEH
                     LAGELPGPALPHPLDVADAGSVEAFRRAVAERFGRVDVLVNNAGIGIFKRLEETSAAE
                     FEQTFRVNVQGAWAATVAFLPRLKESRGLVLMVSSDVSTRTFPTGGSYTASKFALRAL
                     SRTLQQENPELRVLELRPGAVDTWFAGSTPGAPGKDWFLQPPVVAEALRFALRLPPGA
                     RVEELVLRSSGQTPEY"
     gene            9606..>12603
                     /locus_tag="VEL74_05225"
     CDS             9606..>12603
                     /locus_tag="VEL74_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HYG61959.1"
                     /translation="MNERPAWRGRLSPRFSGWQTKWRRLPRRQALGILLVILLVIGAL
                     FSFSLQNCFFDGCPDVDTLASFQPGGAPVLLDRHGEVFADLTPVERKVVPLGSLPSHV
                     PQAFLAVEDKRFHEHGAVDWQRVGGAIIANLRAGGFEQGFSTITMQLARNVFSERIPG
                     QEQTTTRKLLEIRVANEIESKYSKEEILELYLNHIYFGNRAQGIESAAWQYFGRPASQ
                     LTLTQAALLAALPKAPSHYDPRRHPERAIERRNLVIGLMEEQGRVPRLLADQARREPL
                     ELAPAPRHARVESGLAPYFVEQVRRQLEERFGDDLYTQPLRVRTTLDSSTQRAAEEEL
                     RRQLRRIESGELGAFQGARYSASAEPPKEGTDYLQGAVVVLHAQTGDVLAWVGGRDFA
                     HSQFDRVAQARRQVGSAFKPFVYAAALSEGYVLSQPLADRPLRVRLPGGRVWEPKNFT
                     RQYDGEVTLRDALVRSKNVATVRLADAVGLGEVVQVAHAAGITEEGQDDPLPSMALGA
                     VPVSPLELTTAYTGFAGMGRAVEPRLVLSVDTLDGKRIWEPETEAHEVVKPEVAYLIT
                     DVLAEALERGTGIGVRQTGLKVAAAGKTGTTNDGADAWFAGYTPDLVAGVWIGFDRPR
                     AILEDATGGRLAAPLWGNLVERFYQRRPSPKPWPRPERVVAHPVDPVTGLTLENGCSP
                     LRGQSYDELFIAGMEPAAYCPGKEPVPRNGFPAARLEAERENAQEAARLARLRTEEEK
                     LLRQRQAAEQRLAELRRQQEEQQRTQAVEAERLARAEEAREEARKKEAEQERLADAKE
                     VREAKAARDREARREAEEAREAERRKAAEAERLAEARERREREQAREDREEERRRRAR
                     DEESSRQQDEETEVEVELPEEEDEEEEARPASDLSGWWELTNRIDSTNYAAYKGLRLG
                     YRIQLEQEGNRLTGRGQKWSEDGRAVGSGGRTPLTVTGTIDGRNVTLRFTEHGARRTT
                     HGTFNWILSADRTALRGSFSSTAADTS"
BASE COUNT         1892 a         4522 c         4275 g         1914 t
ORIGIN      
        1 cctcgccgcc ggcctggggg cgatgctgct cggcgccgcc cgcggagccg agagccgcga
       61 gcgggccgag cgcaccgccc gggtgacgct ctcggccctg acggaggtgg tggagcgggc
      121 cgccggcggc ggcaccgggg aggccgtgga ggacgccggc gccgccatgg ccgacgagct
      181 cgcggagatc gcgggggatg ctcccgcgga ggagactccc gaggaggata ccggcctcgg
      241 catcgccgac gagttggcgg ccctcgacga agcccccgcc gaagaccaag acgccgaggg
      301 cgaccgggtc cggcaggccg tggccgcctt cgccaaggcc cacccggagg tccaggcggt
      361 ccgcgtcgtc cgcttcgacg ggatcctgct cgaagcgtcc acggcctctg aggacaccgg
      421 cgagcgggcg gcgccgcgcc gcctcgatcg cgacgagaag ccgctctacg atcaggggca
      481 gagcctgcgc gcggccatcg aagggaaccg tgaagagggg gccgcccgcg ctccggagat
      541 cgccgtcgag cggcgggcgg gcggcgtgct gcggctctcg gccccgttcg agaccggcga
      601 cgaggtcgtg ggcatggtgc agatggacac tcggcccctg cagtccgccg cttccttccc
      661 ttggttgccc gccctgctca cctggctcgc gccggtcgtc ctcttcttgc tgctctccct
      721 ggtgctgggc gagcgtcgga ccctcctcgc ggccgtcgcg gtgatcctgc tgctcgctgc
      781 tctgctcggc ttcggacgct gggcccgtac cgcgctggag caggagcggc gcgccaccgg
      841 cgccgccgtc gcctcgacgg tgactgctga ggccgagcgg gccgaggcgc tgctcgccga
      901 gctcggccta ccggccgccg agccgctggc gcccgagctg tgggacgtcg acctctaccg
      961 gcaaccgctc ggcctggtcc agcccggcgg caccctcgac gagggccgtc tcggcgaatt
     1021 cgcgggcgcc gacgccgggc ggctcacccg ctcggtcatc gtcctcgccc tcgccgccct
     1081 ggccgtgctc gccttcatcg gcctcggctt cgcggcgcgc accggcgccg cgctggtccg
     1141 ctaccggaac gcctacgcct acaccctgcc ggcgctgatc gccatgctgg tgctggtgtt
     1201 cttcccgttc ttctacggca tcgccctggc gttcaccaac gccaacgtct acaacaccgg
     1261 gcaatcgatc ttcgaaacct ggatcgggct cgagaacttc gtcaacatcc tttcggacct
     1321 cggcgtggtc gagcggacgg cgcagggatt ggtgatcgat tacaccaact tctactggac
     1381 gctgggcttc accatcgtct ggacgatcac caacgtggcg atcggcgtgt cgctcgggct
     1441 gctgctggcg ctggcgctca acaccaaggg gctcgccttc cggccgatct atcgggtgct
     1501 gctgatcctc ccctgggccg tgcccaccta catcacggcg ctgatctggc aggggatgtt
     1561 ccaccggcag ttcggcgtga tcaatcagat cattcagatg gtgggcggca acccgatcgc
     1621 ctggttcgac gcgccgctca cctcgttcct ggcggtgctg accaccaacg gctggctctc
     1681 cttcccgttc atgatggtgg tctccctcgg cgccctgcag tcgatcccgg cggacctcta
     1741 cgaggcggcg cgggtggatg gcgcgtcgcg ttggcagcag ttcaggtcga tcaccctgcc
     1801 gtcgttgcgc ccggcgctga tccccgcgat catcctgtcg gtcatctgga ccttcaacca
     1861 gttcaacgtg atctatctgg tgtcggcggg ccagccggcg ggcgccacgg agatcctgat
     1921 caccaaagcc tacaagctag ccttcgagaa ttaccggtac ggctacgccg cggcctactc
     1981 gacggtgatc ttcctcatcc tgctggtcta tggcacctgg cagaaccggg tgacccgggc
     2041 gacggaggcc gtcggagaat gacagaatga gccgcaagcg cagccaggag atctcccacc
     2101 tgccgctcca cctgttcctg ctgttgatgg tggctgtgac ggtctatccg atcctgtggg
     2161 tgatcacgat cgctttttcg ggcggccaga gcctggcgat cgccgacatc ccgcaggacc
     2221 cgacgctggc ggaccgggtg cgcgccgtca ccccctggcc ggagcagttc tcgatgtcca
     2281 atttcacgtc gctgtgggcc gaccagccgt tcctgcgctg gctgttcaac agcgctgtgg
     2341 tggccggcct caccaccctg gtcggggtgt tcctgtcgag caccgcggcc tatgccttct
     2401 cccggttccg gttccccgga cggcgggccg ggctcatgtc gttcctggtc tcccagatgt
     2461 tcccggggac gctgatgctc atcccgctct acatcatcgt cgtgcagtgg ctggggctgg
     2521 gatcgtcgtt caccggcctg gtgctggtct actcgacgat cgccatcccc ttctgcgtct
     2581 ggatgatgaa gggctatttc gacaccatcc ccaaggagct ggaggagtcg gcgctgatcg
     2641 acggcgcgtc gcaggcgatg atcttcttcc ggatcgtcct ccccctggcg aagccggcgg
     2701 tggcggtgac cgccctgttc tccttcatga ccggatggaa cgagttcatc atcgccgcca
     2761 ccttcatgaa ccgggaggag atgtacaccg gcccggtcgg gctgcgcttc ttcgtcggcg
     2821 gcttctccca gcagtggggg tacttcgcgg ccggctccat catcgccgcc atcccggtgg
     2881 tgctattgtt cctgttcctg cagaaatacc tggtctccgg cctgacggcc ggcgcggtca
     2941 aaggctgagc ttcacaaaca aggaggtttc atcatgcgca gcactcatcg caagacgttc
     3001 tcagcgctgt cggcattcgg cgccggcctg ctggtcgcgg cctcgctctc cgcccagagc
     3061 gtctcgttca aggacccggc caacgacgac aacggcccgg gcacctacac ctaccccacc
     3121 gacggcgtct acaagcgcgg ctcgttcgac ctcaccggtc tcgacatcga agccaaggga
     3181 aacaaggtgg acttcggcgt gtcgttcaac acgccgctcg aggacccctg gcggatgggc
     3241 accggtttct cggtcgagat ggtcttcatc ttcatcgaca acaaggaggg ggggatgaag
     3301 gagtccttcc ccggcatcaa cgtcaacttc gccgagggca gcgagtggga caagctggtg
     3361 atcctgtcgc cccagcctcc gggccgggta aagaccgagg tcaaggagaa gctgccggcg
     3421 gcgatgcagg cggcggtcat cgtcccctcg cgcgtcaagg gcgccgggcg aacgatctcg
     3481 gccaccgtcg ggctcgacga gctgggcggt ggtgatccgt cgacctgggg ctaccaggtg
     3541 gtcgtccagt ccaacgaggg cttccccgag ggcaaggacc tgatgacccg caaggtcaac
     3601 gagtacgagg gccagcaccg cttcggcggc ggcagcgact acgactgcga cccgcacgcc
     3661 atcgatgtcc tggccggcaa cggcaccggc gccgccgacg aggcggcggc ccagcacgcg
     3721 atgctcaagt acgagtgcgg cccggacggc gccgtcaaga ccgccgcgac gctcaagatg
     3781 gtccgcaagg gctcctgatc gatcatcact ccgctgaggg tagaggagat ttcttcatga
     3841 gatttcagag agggttcgca ttcattctcc tggccacggc ggcgctggcg tcttcggtga
     3901 cgccggcgca cgccgccgac ggccagacgc tcctcatcac cggtgagggg tacatcaagt
     3961 ggctgtgggg caaccagcgc accgacggca gcctctacaa cttcaccacc atcccgaacg
     4021 agggatacgg cgacaacggc cagggcacgg agatcaacct gctgctcgcc tcgcggccgt
     4081 cccgcaacac cgaggtcacg ggccgcatcc agtcccgttt cagccagaac cagtggacca
     4141 acttcggcgg cttcggcggc cgcaacccgg cgttcgagga gcctccgggc gccccctgcg
     4201 tcggcggcga ctgcggcgag ttcgacccgc gctccaacga gtacatcaag atgcgcggcc
     4261 tgaccgcccg ctacaccccg ggctggaagc tggtggacgc catcaccgtc ggctcgaccg
     4321 acctcggcat gttcgacccg tggacgatcg gcaagatccg ctacatcgac cgcgacaacg
     4381 ccaaggcggt gctgctccag gggagcctgg ccaacaactt cggctatgac ctgatccgcg
     4441 tctccctgcc gcggctctgg gccggccccg acttcaacac cggcagctac accgcggccg
     4501 acggcgccta cggcctgcag ctccgcgcca cgccgagccc gctgttcgac ttcgtcggcg
     4561 tcgtcgagac ggtgagcgac ctcgaggtcg acacgctcga tttcgactac gacgacggcc
     4621 gcgacgtcga agcccgcttc agcaacgacg tctacggcgt gcgcttcggc atccacccga
     4681 gcacgctgat cgacatccgc ggcatggcct acttctccca ggcggagtcc gacccgcgct
     4741 tcggcgtccc gtcctccttc ggcctgtccg gcttctcgcc ggtgccggcc ggcgagatcg
     4801 acgacgaggc gttcaaggcg aacgtcgacc tgaacgaccc gttcggcgtc ggcctctcct
     4861 tcaacctgga gtacttcgac atcggcgcgc agtacatctc gatcctggcc gcccgccgcg
     4921 aggcggacgt cctgctgacc gagggcagcg acggcgcctt cgtcttcccc ggcccggaca
     4981 actcggcctt cggcgtcttc gggggcaacc cgacgcgcat cggctacggc ggcttccagg
     5041 gcaacgccca gcaggtggcc acgatcaacg tcgacaacga gttcaccgac ttcgacgagg
     5101 cgatggccga gtcggtcatc ggctggaaag gcttcaccat cgcgcccaac tggagcctcg
     5161 gcggcttcga cctgtcggcc gagtacagct tcatcgacta caacaccaac tggcaggcct
     5221 ggggcgaccc gtcacggggg atcgagagct cgatctatcc caacttcgag tcggacgccg
     5281 gcgtcaactc ctaccgcaac gcctacgcgc cgttccagga gaaggagacc accatcatgg
     5341 tgctccgcgg caagtacgtc ctggacgtag ccaagggcgt cgacctgttc ggcaaggtca
     5401 agctcatcga cgagcaggac gaccggctgg acgacccgcg cttcctcccc taccaggcgg
     5461 gtgactgccc gggcggcggc gcggcgtgca gcaacaacgc ccgcgactac tcgccgggca
     5521 actcgacggc cgcgatctac ggcaacccgc cggtgatcac cggcgccggc ggcgtggtcg
     5581 gctaccagtg ggcgccgttc gacgacatct cggacgacga ccgcgacatg gactaccgga
     5641 tgatccagct cggcggcggc taccagttca ccgacaacct ctacagctcg ctgaccttcg
     5701 agcgctacga cgtcgacctg ctggacggca acaccgcctt ccaggcctac cggctgcact
     5761 cgatgtcgtc gggcgagcac accaagaaca aggtgatcct gttcgcccgc tacacgctcg
     5821 gcggctccga gttcggcttc aactacgagt acaatttcgg cgagttcgac cctgacttcg
     5881 gcggcggctt cgtgccgcag gtgatcagcg acgacaccgc gcgcagcctc ggcctgccgt
     5941 tcggctcgct cggcttcgcc ggccgcttcg gcggctggaa cagcctcgag acacgggagt
     6001 tccggcagaa ccggctgaag gcgttcctga agttccgctt ctaaggagcg atcctggaag
     6061 tttgccggcc cggcggccct ccggccgccg ggcctttttc atcatcgttg gagacgtccc
     6121 catgcacagc agccgcaccc ccgtcctggc cttcactctg gccgccgcgc ttctcgccgc
     6181 ccccgctttc ggccgcagtg agatcttcac cctcaacgat ccgcggggag acgaccatgg
     6241 cgacggcaac tacgtctatc cgctgaacga cgagctcaac gagggcgacc tcgacctgct
     6301 gtcgttgacc gcccgccgcg agggggccgg cacccagttc gagctgacct tcgcccggcc
     6361 ggtcaagacg ccggggcggg tggccatcga ctacagcggc acgcagctcg acaccgtggc
     6421 gcgcttcggc ttctatacgc tgaacgtgga cctctatatc gacatggacc gcgcgccggg
     6481 ctccggcggc acctcgatgc tccccggccg cttggcggag atcgatccgg cgagcgcctg
     6541 ggagcgcgcg gtggtcctca ccccccggcc gcacgaggcg cggggcgagc tcaagcgcat
     6601 gctggcgcgc cagctccggc aggacgcccg cgacgaggac agcgacctcg gacaggcgca
     6661 gctcgaggcg ctgcagaagc agatccccgg cgacgtggag agccgcatct tcttcccgac
     6721 gcaggtccgg gtgcgcggct cgaagatcag cttcttcgtc cccgactcgt tcctgggcgg
     6781 accggccaag gacacctgga gctacgtcgt cgcggtctcc gccgcggacc tcctccagtc
     6841 gttcgacgtc aatcgcgcgc tgggccggca gtcggaggac gaggaggcgc tgatgatcct
     6901 ccccgtctcc ccgggcaagt ggcaggaccg catcggcggc ggccgcgaag gcgccccgct
     6961 ccagccgccg ctcatggaca tcatcgcccc agaaggccgg aagcaggaga caatcctcgg
     7021 cgacttcgat tcgcgggccc ggcggccggc ggtgctgccc ggcgtggtgc ctgccgagca
     7081 gccgaagaag aagtagggct accgcagcac ccagaccccg ctggtccggc ccggcagctg
     7141 gaaggtccaa cctccagccg cctgggccgg ccgacccacc ccggccgagg cgaacagcgg
     7201 atccggcctg cccgagacct cgtaaggccc cgcctgcacc ggctcgcttc ccaggttatg
     7261 gaccaccagg acggtctcct tgccactctt gcggaggaag gcgaggagcg gcgaattggt
     7321 cgtggccggc gtcctgagct cgagctcgcc ggtccgtagg gcgtccgagt tgtggcgcgc
     7381 ccggatgagg tgccggtagt gggtgaggag ggagtccggg tcgccggcct gggccgccac
     7441 gttgttggtc tcgcggcccg gggcgaagtc gtaccagggt ttgccggtgg tgaagccgcc
     7501 ccccggcgag gcgtcccagg gcatgggagt gcgcttgagc tcgtcgctgc tcgacgggcc
     7561 gccattctgc aggcccacct cctcgccgta gtagaggaag ggggcgccgg gcagggtcag
     7621 caggaccgcc gcggccatct tgctccgggc gacccgctcg ccaggatcgg cgccgagctg
     7681 ggtggcggcg cgcaccatgt cgtggttggt gaggaacggc gtgtcgatga tccccgccgg
     7741 gtagagctcg gccatctcgg tcagcttctc ggcgatcggc tgggcgtcgc cggcgttgac
     7801 tccggcgagg atggccgagg cgagcgggaa gttgaagttc atcggcagct cgtcgccgcc
     7861 gcggaccgtg tccgtcgagc catagtaggc ggcgatctcc ggggtctcgg tccagttctc
     7921 ccccaccagc atggcgtccg gcttcgcctg ccgcacggcg gccgagagct ccttcagata
     7981 gacgtgggtc tccggctggt cgttctgtcc ggcgcccggg ccgttggcga agagatgccg
     8041 ggtggcgtcc agccggaagc cgtcgaggcc gcgcccgagc cagagggtcg ccaggcgctt
     8101 catctcctcg cgcaccgcgg gcgtgctgaa gttgaggtcg ggcatgcccc cccagaagac
     8161 gccgtagtag taggcgccgt tcttctcgtg ccaggtgcgg ttgttgcccc cccagggctg
     8221 ggtccagcgc ggatcgtcgg cgcgccagac gtaccagtcg cggcgccctg atgcggggga
     8281 cgaggcggac tcgacgaacc acgggtgttg ggagctgctg tggttcatca cgaagtcgac
     8341 gatgacgcgg atgccccggc ggtgcgcctc gtcgaggaag cgctggaaat cctcggcggt
     8401 gccgtactcg cggtcgatcc gctcgtagtc caccacgtca tagccgtgat agctcgggga
     8461 ctcgaagacc ggcatcagcc agagggcgtc gacgccgagg tcggtcaccg tcttcgggtt
     8521 gccgtcgttc aggtagtcga gcttggaggt gagcccccgg atgtcgccga tgccgtcgcc
     8581 gtccgagtcg gcgaagctgc ggacgaacac ctcgtagaag accgcacccc gtgcccagtc
     8641 gtgctgccac tggacgggcc ccttcgccgg cggggcctcg ggagaggcca gggcgccgac
     8701 ggccggaggg gtggggggcg ctggaggtgc ggcggaaccg cccgcgcagg ccggcagcag
     8761 ggcgaggagg gtggcaagcg gcgccagcag ggtcggcaag gttctcatgg ccgtcattgt
     8821 atgttcagcc ctaccggaga gtgcggtgct aggatcgccc gccatggaca ccccacagcg
     8881 cgtcgccgtc gttaccggtg catctcgcgg cctgggcgcc gcgttcgccc gcgtgctctc
     8941 cgaggagggc ttcgcggtcg ccctgggggc gcgggacgtg gacgcggtcg agcacctggc
     9001 cggcgagttg cccgggcccg ccctgcccca ccccctggat gtggccgacg ccggcagcgt
     9061 cgaggctttc cggcgagccg tcgccgagcg cttcggccgg gtggacgtgc tggtcaacaa
     9121 cgccggcatc ggcatcttca agcggctgga ggagacgtcg gccgccgaat tcgagcagac
     9181 cttccgggtg aacgtccagg gagcctgggc ggcgacggtg gcgttcctcc cccggctcaa
     9241 ggagagccgc ggcctggtgc tcatggtctc ctcggacgtc tccacccgga ccttccccac
     9301 gggcggctcc tataccgcct ccaagttcgc cctccgcgcc ctcagccgca ccctgcagca
     9361 ggagaacccg gagctgcggg tcctggagct gcgccccggt gccgtggaca cctggttcgc
     9421 cggctctacc ccgggcgccc cgggcaagga ctggttcctc cagccgcccg tggtcgccga
     9481 ggccctccgc ttcgccctcc gcctcccgcc cggcgcccgt gtcgaggagc tggtgctgcg
     9541 gagcagcggc cagacgccgg agtactgagc cgacctggca agccccttgc tccccctggc
     9601 tcggcgtgaa tgaacgaccg gcctggcgag gccgtttatc gccccggttc tcgggatggc
     9661 agacgaagtg gcggcgcctg ccgcggcggc aggcgctggg aatcctcctg gtgatcctcc
     9721 tcgtcatcgg cgccctcttc tccttctcct tgcagaactg cttcttcgac ggctgtcccg
     9781 atgtggacac gctggcgtcc ttccagccgg gcggcgcgcc ggtgctcctc gaccgccacg
     9841 gcgaggtctt cgccgaccta acccccgtcg agcgcaaagt cgtccccctg ggctcgctcc
     9901 cctcccacgt cccccaggcg ttcctggcgg tggaggacaa gcgcttccac gagcacgggg
     9961 ccgtcgactg gcagcgggta gggggcgcca tcatagccaa cctgcgggcc ggcggcttcg
    10021 agcaggggtt cagcaccatc accatgcagc tagcgcgcaa cgtcttttcc gagcgcatcc
    10081 ccgggcaaga gcagaccacc acccgcaagc tcctggagat ccgggtggcc aacgagatcg
    10141 agtccaagta cagcaaggag gagatcctcg agctgtacct gaaccacatc tacttcggca
    10201 accgcgcgca gggcatcgag tccgcggcct ggcaatactt cggccggccg gcgtcccagc
    10261 tcactctcac ccaggccgcc ctgcttgcgg cgctccccaa ggcgcccagc cactacgacc
    10321 ctcggcgtca cccggagagg gccatcgagc ggcgaaatct ggtcataggc ctcatggaag
    10381 agcaggggcg cgtgccgcgg ctgctggccg atcaggcgcg ccgggagccg ctggagctcg
    10441 ccccggcgcc gcggcacgcg cgcgtcgagt ccgggctcgc cccctacttc gtggagcagg
    10501 tccgccgcca gctcgaggag cgcttcggcg acgacctcta cacccagccg ctgcgcgtcc
    10561 gcactaccct ggactccagc acccagcgcg ccgccgagga ggagctgcgc cgccagctcc
    10621 ggcgcatcga gagcggtgag ctgggcgcct tccagggcgc ccgctactcg gcctcggcgg
    10681 agccgcccaa ggaggggacc gattacctcc agggcgcggt ggtggtcctc catgcccaga
    10741 ccggcgacgt gctcgcctgg gtgggtggcc gcgacttcgc ccactcccag ttcgaccggg
    10801 tcgcccaggc gcgccgccag gtgggcagcg ccttcaagcc gttcgtctac gcggcggccc
    10861 tgtcggaggg atacgtcctc tctcagcctc tggccgaccg gccactgcgc gtgcggctgc
    10921 ccggcggccg ggtctgggag ccgaagaact tcacccgcca atacgatggg gaggtcacgc
    10981 tgcgcgacgc cctggtgcgc tccaagaacg tggccaccgt ccgcctcgcg gatgcggtgg
    11041 gcctgggcga ggtggtccag gtcgcccacg cggcggggat caccgaggag gggcaggacg
    11101 atcccctccc ctccatggcg ctgggcgccg tgccggtatc ccccctggag ctgaccaccg
    11161 cctacaccgg cttcgccggc atgggccggg cggtcgagcc ccgcctcgtc ctctccgtcg
    11221 acaccctgga cgggaagcgg atctgggagc cggagaccga ggcacacgag gtggtcaagc
    11281 cggaggtggc ctacctgatc accgacgtgc ttgccgaggc gctggagcgg ggtaccggga
    11341 tcggcgtgcg ccagaccggc ctgaaggtcg ccgccgccgg caagaccggc accaccaacg
    11401 acggcgccga cgcctggttc gccggctaca cgcccgacct ggtggcgggg gtctggatcg
    11461 gcttcgaccg cccgcgcgcc atcctcgaag acgccaccgg cggccgcttg gccgccccgc
    11521 tctggggcaa cctcgtcgag cgcttctacc agcggcgccc cagccccaag ccctggccgc
    11581 ggccggagcg tgtggtggcc catcccgtcg acccggtaac cggcctgacc ctggaaaacg
    11641 gctgctcccc cttgcggggc cagtcctacg acgagctgtt catcgccggg atggagcctg
    11701 cggcctactg ccccggcaag gagccggtgc cccgcaacgg cttccccgcc gcccgcctgg
    11761 aagccgagcg cgagaacgcc caggaggccg cccgcctcgc ccgtctgcgc accgaggagg
    11821 agaagctcct gcgccagcgg caggccgcgg agcagcgcct cgccgagctc cgccggcagc
    11881 aggaggagca gcagaggacg caggcggtcg aggccgaaag gctcgcccgc gccgaagagg
    11941 cccgggagga ggcccggaag aaagaggcgg agcaggagcg cctcgccgac gccaaggagg
    12001 tgcgggaggc gaaggcggcc cgcgaccgcg aggcccggcg cgaagccgag gaggcccggg
    12061 aggcggagcg ccgcaaggcc gccgaggccg agcgcttggc agaagcccgc gagcggcgcg
    12121 agcgcgaaca agcccgggag gaccgcgagg aggagcggcg ccggcgcgcg cgggacgagg
    12181 agtcgtcccg gcagcaggat gaggagaccg aggtggaggt cgagcttccc gaggaagagg
    12241 acgaggaaga ggaagccagg ccggcttccg acctctccgg ctggtgggag ctgaccaacc
    12301 gcatcgactc caccaactac gcggcctaca agggcctgcg cctcggctac cgcatccagc
    12361 tcgagcaaga gggcaaccgt ctcaccggcc gcggccagaa gtggagcgag gacggccgcg
    12421 ccgtcggctc cggcggccgc actccgctca ccgtcaccgg caccatcgac ggccgcaacg
    12481 tcaccctgcg cttcaccgag cacggcgccc gccgcaccac ccacggcacc ttcaactgga
    12541 ttctctcggc ggaccgtacc gccctgcgcg gcagcttctc cagcaccgcc gccgacacca
    12601 gcg
//
LOCUS       DASPJI010000199        12409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_354037, whole genome shotgun sequence.
ACCESSION   DASPJI010000199 DASPJI010000000
VERSION     DASPJI010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12409)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12409
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354037"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1726)
                     /locus_tag="VEL74_05230"
     CDS             complement(<1..1726)
                     /locus_tag="VEL74_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61960.1"
                     /translation="MRRAGLLLLAGILAGSPFFPARAQGPLLTQGPAGDWRTVTTEHY
                     RLHYPAPSEAWARYAAVRLESIHERVVQEVGYDPPEVVDVLVSDPVANANGMAYPFLG
                     WPRMVLWTSPPGPESTIGHYLDWPELLIVHEATHLVHLLRPSRNPARQMLTRLVPAGP
                     ITLGAPRWVTEGYATVVEGRLTGSGRPHSDLRAAMLRRWAQGGKLPSYGQLANDSRSW
                     QGMSMAYLLGSAYLEWLEERTGPGSLRNLWARMTARRARSFDEAFQGVFGDSPANLYD
                     RFRAELTWRAMEVERRLGERLEGELWQDLSWTTGTPAVSPDGEKLAIVLDSRDDPARL
                     VVWPTGPNTEAEEEYREEQEEILERDPQDVPAVRDRPLPREPLHTLESRNGIEPTTPR
                     WLRDGRSILFVRFEPDGEGFIHPDLFLWALESGEVRRVTRGADVRDPDPAPDGTWAVA
                     VRNRHGLSQLVRVDLRTGAVEDLTEPAVETVYDRPRISPDGRRLAVVRHRQGAPEANQ
                     RAWRVVIRDLAGGAGGAETELAPPAGDIGGTLSSPAWSADGRTVYAVVGSGGFIDVWA
                     FPVDGGAPV"
     gene            complement(1747..1983)
                     /locus_tag="VEL74_05235"
     CDS             complement(1747..1983)
                     /locus_tag="VEL74_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HYG61961.1"
                     /translation="MADSKITVMKNGPFRLEGENLQILDPTGQAYGLGGRTVVSLCRC
                     GASSNKPFCDGSHNHHGFQDEPQARDLPPKKPAV"
     gene            2081..3184
                     /locus_tag="VEL74_05240"
     CDS             2081..3184
                     /locus_tag="VEL74_05240"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HYG61962.1"
                     /translation="MENEAPIHDRTPPPGEVPDEPSAAKRWAGHLLKLALAFLGIYLT
                     WRLVAGIDWSDLSRRVAGASWPLLALAAVLLLARYSLWDWRFRLAARRVAETRTGPAL
                     GFFVLMASAALNLITPSARLIGGLMRARYFAHAAARPFGMMYGVVLYDQVAHHVVMST
                     CTCLAVVAAAFAMGRTALGLGALAVLAAAAVAAVLWSRRRGTGENPLVRFLARRAEAA
                     QGRSQRLYTHGHEAVGIFVRLLSDVPLRGHAALLGAGFYTLNILAQWLVFLALGQRVD
                     PMVTLIGVSLGAAAGMLTGTPGGIGTTEAAMVASFTALGMDPVDAAAGSLLFRGLHYA
                     GVLAVGLPALAGLEIQMSRGAAAPADGAPRAEP"
     gene            3214..4365
                     /locus_tag="VEL74_05245"
     CDS             3214..4365
                     /locus_tag="VEL74_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYG61963.1"
                     /translation="MDDATLERLRREEFPVTERWAYMNHAGIAAPPRRAAERMAAAVA
                     GASRSGDREWLDRNAEAERVRRQAARILGARDPREVAFVENTSTGLSLVAEGIDWRPG
                     DNVVSAALEFPSNVYPWMRLMDQGVEYRLAGERDGRLDLDEIEGLIDERTRAVALSWV
                     QYASGFRAGLRRLGAVCRERDILFIVDVIQGLGGLTLDVERDLVDVAAASGHKWLLGP
                     EGIGLLYVSDRVVERLRPARSGWRSMRHISDWTKLEVDWNEGAKRFESGTLNVYGIHG
                     LGGALDILLEVGPETVERRVLALAGRAARIFQERGLTLVSSRRPGETSGIVTAIPRPG
                     GPDADALARGLAERGVVLAQRAGRLRAAPHFYNTDEEMDRLEAGLRDLV"
     gene            4612..5037
                     /locus_tag="VEL74_05250"
     CDS             4612..5037
                     /locus_tag="VEL74_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HYG61964.1"
                     /translation="MSAKVFVGNLDFNATKEQVQSLFEQVGPVQDVFLPMDRETGRPR
                     GFAFVEYESESDAEKAIAQFNGQELLGRTLRVNAAEDRPRRTGGFGGGGGGGERSFSG
                     GHGFGGPGGYGGGGGGGGGFGGGKPKGSRRNIRAKKRSL"
     gene            complement(5200..6132)
                     /locus_tag="VEL74_05255"
     CDS             complement(5200..6132)
                     /locus_tag="VEL74_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61965.1"
                     /translation="MEHLEKARESAYWDLYREGVDALHAARLAEAETVLQQALEEARR
                     RRLRLLADRAYCNWAAVRIERGLPAVETGGVVEGLSAILGRSRDLKARQLAAYDLACL
                     YRVQGRNRPGRLYAEIAERLAATGGDVQSRAVSRHLLGLLWLGDGRMEQARRCIEEAL
                     EMGLQGGRGQGAVVLSTLGYCLSLTGEPARGMHLLEQSLASLGEPLSPLYEPSLHLNI
                     GFACLELGDLDAAIHHGEHVLRLEHREGRDGRSGDESKYARYLVGEALVQRGSAGQAR
                     EHFEVLRQEFYPEIPDLPELLLACRTHSLLNWLA"
     gene            6206..6922
                     /gene="pepE"
                     /locus_tag="VEL74_05260"
     CDS             6206..6922
                     /gene="pepE"
                     /locus_tag="VEL74_05260"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HYG61966.1"
                     /translation="MATQRRLLLISNSTNHGQGYLDHCLAEIDDFLAPARRLVFVPFA
                     LADHRGYGAKVARRLADIGVEAATLTPDAAGERLLREAEAVFVGGGNTFRLLKTLQDT
                     GHLEILRERARAGMPYIGSSAGTNLATPTIRTTNDMPIVEPRSLDALRLVPFQINPHY
                     LDADPSSTHMGETREERLMQFHEENETPVVGLREGAWVRAEGDAGRLGGQRGARIFRR
                     GQTPEERRAGASLDDLLRGS"
     gene            7684..9621
                     /gene="acs"
                     /locus_tag="VEL74_05265"
     CDS             7684..9621
                     /gene="acs"
                     /locus_tag="VEL74_05265"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HYG61967.1"
                     /translation="MIPVKPHVAERAHIQSLEQYREMYERSIEDPAGFWREQSAILDW
                     FHPPRNIVDMDPDEVDFSWFSGARLNACYNCVDRHLTTQPEKTAIIWAGDEPGEYRHI
                     SYREVKHNVARFANVLLHHGVRKGDRVCIYLPMIPELAYSMLACARIGAVHSVVFAGF
                     SAESLRGRILDAGCKLVLTANEGLRGGKRIPLKATVDQAVEGLGIVEKVLVARRTEKE
                     VPMRSGRDLWLEEETARQRSTCPIEWMSAEDPLYILYTSGSTGRPKGVLHTTAGYLVY
                     VAATHKYVFDYHPGDIYFCAADVGWVTGHSYIVYGPLANGATTVMFESVPTYPDAGRY
                     WRIVDDLGVNIFYTAPTAIRAIARSGDEWVKRYKRTSLRVIGSVGEPINPEVWLWYHD
                     VVGEGRCAVVDTWWQTETGGILITPLPGATPCKPGSATLPFFGVRPVLVDDQGNELSG
                     NDVSGNLCLRGTWPGQARTIYGDHRRFRETYFSQFPGLYFTGDGCRRDKDGYYWITGR
                     VDDVLNVSGHRLGTAEIESALVAHDAVAEAAVVGYPHEIKGTGIYAYVHVTPDYESWD
                     EEELVGALKEQVRHAIGAIATPDRIQVVAGLPKTRSGKIMRRILRKIAQGEYEDLGDI
                     TTLADPGVVAKLVDDHRARSG"
     gene            9795..>12409
                     /locus_tag="VEL74_05270"
     CDS             9795..>12409
                     /locus_tag="VEL74_05270"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HYG61968.1"
                     /translation="MTLGIAVAGYTRLRSLTATIARLEGRIQALEVRPREGAETPAPP
                     LPAVAAVAPPPPAAAVSVPVPPPPAPEPIPSPPPVLTTPSPAGPFAGVGERLRALSAQ
                     REAGAAKPAASLEERLGARLPVWIGSIALALAGAFLVKYSFDQGFLSPVVRVALGVAF
                     GVALLAGGEWMRRSSPGISQGLSAAGIADLFACFLAGVHLYQLISPAVGFAFMALTAL
                     VAVLLSLRHGPMVALIGLAGGFLTPALIRAGEPDVRNLFAYLLVLVIAVAVVSGQRGW
                     RWVTGLALAGGIVWVLVWLAGPFQTDDAPWLSLFLVALSVAAVVSDLRRRALAPDGAG
                     GVPGGTWLSWGALVASLGVIAIVTGASGYSTLEWAFLGLLAGSILILARLHTSFEGLG
                     WVAAAAPALLLAAWGYDLPDVGSSGAGRFLRTVLAFGALFAIGGYLAHRGAPRPGRWA
                     SLSAASAVVYFLLAWATTVQDVDLPWGGLALGLAALYIVALLPVARQRRDHPHLDAPL
                     AALAVAVTVFVSLAVPLELERQWLTVAWALEVAALTWLAGRFRLPALTLLARLLALAV
                     LVRLLLNPEVFNYPIGEHPVFNWIVYGYGLPLLAFIAAAVLARRQGRTQPATELEWGA
                     LAITLVFSTLWVRQLFHPGEPGRVETGFVELGTLAALWIALGLLILVLSRRVPQLLPS
                     GGEGRPAQATGSVGGGEGGSAHEGAEQGAPPLLASLESGGRAFLLLGCLTALFGAGFL
                     QNPLWTHEPAGSLPIFNLLLWAFGAPAALLALGARELSRRASRLPAVLTIAALALTFL
                     LVSLEVRQAFQGTYLDTGVTSAAERYTYSAAWIAFGIALLVIGTARKGKALRYASLAV
                     MLLTVGKVFLYDTANL"
BASE COUNT         1616 a         4510 c         4438 g         1845 t
ORIGIN      
        1 ggaccggcgc ccccccgtct accggaaagg cccatacgtc gatgaagccg ccgctcccca
       61 ccaccgcgta gacggtgcgg ccgtcggcgc tccaggccgg agaggagagg gtgccgccga
      121 tgtctccggc aggaggcgcc agctcggtct ccgcgccgcc cgcgccgccg gcgagatcgc
      181 ggatcaccac ccgccaggcc cgctggttgg cctccggggc tccctggcgg tggcggacca
      241 cggcgaggcg ccggccgtcg ggcgagatgc gcggccggtc gtagaccgtc tcgaccgccg
      301 gctcggtcag gtcctcgacc gcgccggtcc gcaggtccac gcgcacgagc tgcgagaggc
      361 cgtggcggtt gcgcacggcc acggcccagg tgccgtcggg cgccgggtcg gggtcgcgca
      421 cgtcggcgcc ccgggtgacg cgccgcacct cgccgctttc gagagcccag aggaacagat
      481 cggggtggat gaacccctcg ccgtcgggct cgaagcggac gaagaggatg gaccggccgt
      541 cgcgcagcca gcgcggggtg gtgggctcga tgccgttcct cgactccagg gtgtggagcg
      601 gctcgcgggg gaggggccgg tcgcggaccg ccggcacgtc ctgggggtcg cgttcgagga
      661 tctcctcctg ctcctcgcgg tactcctcct cggcctcggt gttcggcccc gtgggccaga
      721 ccaccaggcg ggcggggtcg tcccgggagt cgaggacgat ggcgagcttc tcgccgtccg
      781 gcgagacggc gggggtgccg gtggtccagg agaggtcctg ccagagctcc ccctccaacc
      841 gttccccgag ccgccgctcc acctccatgg cgcgccaggt cagctcggcg cggaagcggt
      901 cgtagaggtt ggcgggggag tcgccgaaga cgccctggaa ggcctcgtcg aagctgcggg
      961 cgcgccgcgc cgtcatccgc gcccagaggt tgcgcaggct ccccgggccg gtgcgctcct
     1021 ccagccattc gagataggcg gagccgagca gataggccat ggacatcccc tgccagctcc
     1081 gcgagtcgtt ggcgagctgg ccgtagctcg gcagcttccc cccctgcgcc cagcgccgca
     1141 gcatcgccgc ccgcaggtcg ctgtgcgggc gccccgagcc ggtgagccgc ccctcgacca
     1201 ccgtggcata gccctcggtg acccagcgcg gcgcccccag ggtgatgggg ccggcgggga
     1261 ccagccgggt cagcatctgc cgcgccgggt tgcgcgaggg ccgcagcagg tggaccaggt
     1321 gggtcgcctc gtggacgatg agcagctcgg gccagtccag atagtggccg atggtggact
     1381 ccgggccggg cgggctggtc cacagcacca tgcgcggcca ccccaggaag gggtaggcca
     1441 tgccgttggc gttggcgacc gggtccgaga ccaggacgtc caccacctcg ggcgggtcgt
     1501 accccacctc ctggaccacc cgctcgtgga tggactccag gcggacggcg gcgtaccggg
     1561 cccaggcctc ggacggcgcc gggtagtgga gccgatagtg ctcggtggtg acggtgcgcc
     1621 agtcccccgc gggcccctgg gtgagcaggg ggccctgggc ccgggccggg aagaacggcg
     1681 atccggcgag gatcccggcg aggagcagga gaccggcgcg gcgcatgttg acctcgatcc
     1741 cttccgtcag accgccggct tcttcggcgg caggtcgcgg gcctgcggct cgtcctggaa
     1801 gccgtggtgg ttgtggctgc cgtcgcagaa cggcttgttg ctcgacgcgc cgcagcggca
     1861 gagcgaaacg acggtgcggc cgccgagccc gtaggcctgg ccggtaggat cgaggatctg
     1921 caggttctcc ccctcgagac ggaaggggcc gttcttcatc acggtgatct tgctgtctgc
     1981 catggcggtt ctctcctctc aatggaggag agccttcctt atagaagagg ccacgtcaag
     2041 cgacagccag tggcgtgcgc tatccttgcc gcccctgacc atggaaaacg aagcaccgat
     2101 ccacgaccgc accccgcctc ccggcgaggt gccggacgag ccctccgcgg cgaagcgctg
     2161 ggccggccac ctgctgaagc tggcgctggc cttcctgggg atctacctca cctggcggct
     2221 ggtggcgggc atcgactgga gcgacctgtc gcgccgggtg gcgggggcga gctggccgct
     2281 gctcgccctg gcggcggtcc tcctcctcgc ccgctactcg ctctgggact ggcggttccg
     2341 gctggcggcg cggcgggtgg ccgagacgcg gaccgggccg gcgctcggct tcttcgtcct
     2401 catggcctcg gccgccctga acctgatcac cccttcggcc cggctgatcg gcggcctgat
     2461 gcgggcgcgc tacttcgccc acgccgccgc ccgcccgttc ggcatgatgt acggcgtcgt
     2521 gctctacgac caggtggcgc accacgtggt gatgagcacc tgcacctgcc tggcggtggt
     2581 ggcggcggcc ttcgccatgg ggcggaccgc cctcgggctg ggcgccctgg cggtgctggc
     2641 cgccgcggcc gtcgcggccg tcctctggag ccggcgccgg gggaccgggg agaacccgct
     2701 ggtccgcttc ctggcccgcc gcgccgaggc ggctcagggg cggtcgcaac ggctctacac
     2761 ccatggccac gaagcggtgg ggatcttcgt ccgcctgctt tcggacgtgc cgctgcgcgg
     2821 gcacgcggcc ctgctggggg ccggcttcta caccctcaac atcctggcgc agtggctggt
     2881 cttcctggcg ctggggcagc gggtggatcc catggtgacg ctgatcggcg tctccctggg
     2941 ggccgcggcg gggatgctca ccggcacgcc cggggggatc ggcaccaccg aggcggccat
     3001 ggtggcctcc ttcaccgcgc tgggcatgga cccggtggac gccgccgccg gcagcctgct
     3061 cttccgcggc ctccactacg cgggcgtgct cgccgtcggc ctgccggcgc tggcggggct
     3121 ggagatccag atgagccgcg gcgccgccgc gccggccgac ggggcgccaa gggccgaacc
     3181 gtgatatcga tatccgatcc cttgggagga gagatggacg acgcgacgct ggagcgcctg
     3241 cgccgggagg agttcccggt caccgagcgc tgggcctaca tgaaccacgc gggcatcgcg
     3301 gcgccgccgc gccgcgccgc cgaacggatg gccgccgccg tggccggcgc ctcgcgcagc
     3361 ggcgaccgcg aatggctgga ccgcaacgcc gaggccgagc gggtgcgccg gcaggccgcc
     3421 cgcatcctcg gcgcccgcga cccccgcgag gtggccttcg tcgagaacac ctccaccggc
     3481 ctctccctgg tggccgaggg gatcgactgg cggccgggcg acaacgtggt cagcgcggcg
     3541 ctggagttcc cgtccaacgt ctacccgtgg atgcgcctga tggaccaggg ggtcgagtac
     3601 cggctggccg gggagcgcga cggccgcctc gacctcgacg agatcgaggg gctcatcgac
     3661 gagcgcaccc gggccgtggc cctctcctgg gtccagtacg ccagcggctt ccgcgccggc
     3721 ctgcgccgcc tcggcgccgt ctgccgggag cgggacatcc tcttcatcgt cgacgtcatc
     3781 caggggctcg ggggtctcac cctcgacgtc gagcgcgacc tggtggacgt cgccgccgcc
     3841 tccggccaca agtggctgct cggccccgag gggatcggcc tgctctacgt ctccgaccgg
     3901 gtggtcgagc gcctgcgccc ggcgcgctcg ggctggcgct cgatgcgcca catctccgac
     3961 tggacgaagc tggaggtcga ctggaacgag ggggcaaagc gcttcgagag cggcaccctc
     4021 aatgtctacg gcatccacgg cctcggcggc gccctggaca tcctgctgga ggtggggccc
     4081 gagaccgtgg agcgccgcgt cctcgccctg gccggccgcg ccgcccgcat cttccaggag
     4141 cgcggcctga cgctggtgag ctcgcgccgc cccggcgaga cctcgggcat cgtcaccgcc
     4201 atcccgcgcc ccggcgggcc ggacgccgac gccctggccc gcggccttgc cgagcggggg
     4261 gtcgtcctcg cccagcgcgc cggccgcctg cgcgccgcgc cccacttcta caacaccgat
     4321 gaggagatgg accggctgga ggcaggattg cgcgatctgg tgtgacggtg gctgtgacgg
     4381 gcgggaaagt ggacaagagg acgcgcaacc tggacaatac ctctcgacgg atctcatggc
     4441 gctcccgcgg gcgcgatgag cggcggccag ggcaccggtc gccgcgggcg gcagagatcc
     4501 ggcccggaaa ggccgggccg gccgtcctcc ggcgtggagt agaatgggtg agcggagcag
     4561 gtcgagaacc caatcaaggg gcgcggcaga gccgcagaag aggaaagaga aatgagtgcc
     4621 aaggtcttcg tgggcaacct ggacttcaac gcgacgaagg agcaggtgca gtcgctgttc
     4681 gagcaggtgg gcccggtcca ggacgtcttc ctcccgatgg accgggagac ggggcgtccc
     4741 cgcggctttg ccttcgtcga gtatgagtcc gagtccgacg cggagaaagc catcgcgcag
     4801 ttcaatggcc aggagctcct cggccgtacg ctccgcgtca acgccgcgga ggaccgtccg
     4861 cgccgcacgg gcgggttcgg cggcggcggc ggcggcggtg agcgctcgtt ctccggcggc
     4921 cacggcttcg gcggccccgg cggctacggt ggcggcggtg gcggcggcgg tggcttcggc
     4981 ggcggcaagc ccaaggggag ccgccgcaac atccgcgcga agaagcgcag cctctagaga
     5041 cgtccccccg cgggacgtcc cccgcgcagt gctccgggcc tcccggttcc cgggaggcgg
     5101 tcgtgtcctg attttctgat ttcctccgca ccgtgcttcg ctccacgcct cgacccctcg
     5161 cggcgggggc gaagcccgtt ccagctcagg agaggcgggt caggcgagcc agttcagcag
     5221 ggagtgggtg cggcaggcga ggagcagctc cgggaggtcg gggatctcgg ggtagaactc
     5281 ctgccgcagc acctcgaagt gctcccgcgc ctggccggcg gaccccctct ggaccagggc
     5341 ctccccgacg agatagcggg cgtacttgct ctcgtccccg ctgcggccat cgcgcccctc
     5401 ccggtgctcg agccggagga cgtgctcgcc gtggtggatg gcggcgtcga ggtcgcccag
     5461 ctccaggcag gcgaagccga tgttcaggtg gagcgacggc tcatagaggg gcgacagggg
     5521 ctcgccgagg gaggccaggc tctgctccag gaggtgcatg ccgcgcgccg gctcgccggt
     5581 gaggctcagg caatagccca gcgtgctgag gacgacggcg ccttggccgc gccccccttg
     5641 cagccccatc tccagcgcct cctcgatgca ccggcgggcc tgctccatcc gcccgtcgcc
     5701 cagccagagg aggccgagga ggtggcggct caccgcccgg ctctggacgt cgccgccggt
     5761 cgcggccagc cgctcggcga tctcggcata gagccggccg gggcggttcc tgccctgcac
     5821 ccggtagagg caggcgaggt cgtaggcggc gagctggcgc gccttgaggt cgcggctgcg
     5881 gccgaggatc gccgagagcc cctcgacgac gcccccggtc tccacggcgg gcaggccgcg
     5941 ctcgatgcgc accgcggccc agttgcaata ggcacggtcg gcgagcaggc gcaggcgccg
     6001 gcggcgggcc tcctcgagcg cctgctgcaa gaccgtctcg gcctcggcca gccgcgccgc
     6061 gtgcaacgcg tcgacgccct ccctgtacag atcccagtag gcggactcgc gagccttttc
     6121 cagatgctcc atggtgctac ctcgaaaact gcttggatgg tctcagggta gcatactctc
     6181 tccgatggac cggaaaggag aggatgtggc gacccaacgc aggctgctgc tgatcagcaa
     6241 ctccaccaac cacggccagg gctacctcga ccactgcctg gccgagatcg acgacttcct
     6301 cgcgccggcg cggcgcctgg tcttcgtccc cttcgccctc gccgaccacc ggggctacgg
     6361 cgccaaggtc gcgcggcggc tggcggacat cggcgtcgag gcggcgacgc tgacgccgga
     6421 cgccgcgggc gagcggctgc tccgggaggc ggaggcggtc ttcgtcgggg gaggcaacac
     6481 cttccggctg ctgaagacgc ttcaggacac cggtcatctg gagatcctgc gtgagcgggc
     6541 ccgggccggc atgccctaca tcggcagcag cgccggcacc aacctggcga cgccgaccat
     6601 ccgcaccacc aacgacatgc ccatcgtcga gccgcgctcg ctggacgccc tgcggctcgt
     6661 ccccttccag atcaaccccc actatctgga cgccgatccc tcctccaccc acatgggcga
     6721 gacccgggag gagcggctga tgcagttcca cgaggagaac gagacgccgg tggtgggcct
     6781 gcgggagggc gcctgggtcc gcgccgaagg ggacgccggc cgcctcggcg gccagcgggg
     6841 cgctcggatc ttccggaggg gccagactcc cgaggagcga agggcggggg cgtcgctgga
     6901 cgatctactg cggggatcct gatatgaagc cgccgggcgg acacggcggc gttctgaagg
     6961 aggcatagcc ggcccgccga aggctccgac agctcgctgg aagcttcgcc agcgcgttgg
     7021 aagctccgcc agggcgttgg aagcttccga cagctcgtcg aagactccgc tggcgcgccg
     7081 gaagcttcga cggcccgccg aaggcttcgg cggcgcgatg gaagcttcgc cagggcgttg
     7141 gaagctccgg cggcgcgttg tagataccca acgcgctgtc gaaaagttca acagctcgtc
     7201 gaaggttgcg gcgagctggc ggaggttgca acgatgcgtc gaggattctg agggcgcgcc
     7261 agaggttacg acgctctgtc gaagagtacg acggcgcgcc gcttcgccca ccgccgggca
     7321 ggttgccgcc gctggcgcag cgcccaagtc agcgggatgg ggcgaccggc ttggtcctcg
     7381 tcggagcggt gcgggggacg gggcgtgccg tggattgtgc gatcttgatc aacccgctga
     7441 gagcctcgtt cacggccttg tcgctgggaa agaccctggc gatgacgtcc cctcctccgt
     7501 atgcgacacg atcccaggaa ggtccgggtc ggaacccgta acggctagcg ggtcgcgaaa
     7561 cgctgtagat gcttctctga acgagacgcc atgcttgcgc gtgttgaggg tcgccttctc
     7621 cgagtcccat gcgaatctca caacatcatt gtagtcaacg cccgcggggt tagaatccgg
     7681 gccatgatcc ctgtcaagcc ccacgtcgcc gagcgcgccc acatccagag cctggagcag
     7741 taccgggaga tgtacgagcg gtccatcgag gaccccgcgg ggttctggcg cgagcagtcc
     7801 gccatcctcg actggttcca tccgcctcgc aacatcgtcg acatggaccc cgacgaggtc
     7861 gacttctcct ggttctccgg cgcgcggctc aacgcctgct acaactgcgt cgaccgccac
     7921 ctcaccaccc agccggagaa gaccgccatc atctgggccg gcgacgagcc gggcgagtac
     7981 cggcacatct cctatcgcga ggtgaagcac aacgtcgccc gcttcgccaa cgtgctcctc
     8041 catcacggcg tgcgcaaggg ggaccgggtc tgcatctacc tgccgatgat cccggagctc
     8101 gcctactcga tgctcgcctg cgcccgcatc ggcgccgtcc actcggtcgt cttcgccggc
     8161 ttctccgccg agtcgctgcg cggccgcatc ctcgacgccg ggtgcaagct ggtcctcacc
     8221 gccaacgagg ggctccgcgg cggcaagcgc atcccgctca aggcgacggt ggaccaggcg
     8281 gtcgaggggc tggggatcgt cgagaaggtg ctggtcgccc ggcgcacgga gaaggaggtg
     8341 ccgatgcgct ccggccgcga cctctggctc gaggaggaga ccgcccggca gcgctccacc
     8401 tgcccgatcg agtggatgtc cgccgaggac ccgctctaca tcctctatac gtcgggctcg
     8461 accggccgcc ccaagggggt gctccacacc accgccggct acctggtcta tgtcgccgcc
     8521 acccacaagt acgtcttcga ctatcacccc ggcgacatct acttctgcgc cgccgacgtc
     8581 gggtgggtca ccggccacag ctacatcgtc tacgggccgc tcgccaacgg cgccaccacg
     8641 gtgatgttcg agtcggtccc cacctacccg gacgccggac gctactggcg gatcgtcgac
     8701 gacctggggg tcaacatctt ctatacggcg ccgaccgcga tccgcgccat cgcccgctcc
     8761 ggcgacgagt gggtcaagcg ctacaaacgg acctcgctgc gcgtcatcgg gtcggtgggc
     8821 gagccgatca accccgaggt ctggctctgg taccacgacg tggtggggga ggggcgctgc
     8881 gccgtggtcg acacctggtg gcagaccgag accggcggca tcctgatcac gccgctcccc
     8941 ggcgccaccc cgtgcaagcc ggggtcggcc acgctgccgt tcttcggcgt gcggccggtg
     9001 ctcgtcgacg accaggggaa cgagctctcc ggcaacgacg tctcgggcaa cctctgcctg
     9061 cgcggcacct ggccgggaca ggcgcgcacg atttacggcg accaccggcg gttccgggag
     9121 acctacttct cgcagttccc cggcctctac ttcaccggcg acggctgccg ccgcgacaag
     9181 gacggctact actggatcac cggccgggtg gacgacgtgc tgaacgtctc cggccaccgg
     9241 ctggggacgg ccgagatcga gagcgcgctg gtggcgcacg acgccgtcgc cgaggcggcg
     9301 gtcgtcggct acccgcacga gatcaagggg accggcatct atgcctacgt ccacgtgacc
     9361 cccgactacg agagctggga cgaggaggag ctggtcggcg cgctcaagga gcaggtgcgc
     9421 cacgccatcg gcgccatcgc caccccggac cgcatccagg tcgtcgccgg gctgccgaag
     9481 acccgctcgg gcaagatcat gcggcgcatc ctgcgcaaga tcgcccaggg ggagtacgag
     9541 gatctggggg acatcaccac cctggccgac cccggtgtgg tcgccaagct ggtggacgat
     9601 catcgcgccc gttccggcta agtggatgaa gaaggggctt gacccgctgg agacgtggta
     9661 tgatggaacc ctgttcccga ggcccctctc tccagctggt tcgctgtccg gtgccggacg
     9721 cgggtggcgg tagcctgact tcaggctcct tcaatctcta gcaaatggaa tgcctggcga
     9781 cactggcggt cttcgtgacc ctcgggatcg cggtcgccgg ctacacccgt ctgcgcagcc
     9841 tcacggccac catcgcacgg ctcgaagggc ggattcaggc gctcgaggtc cggccgcggg
     9901 agggcgccga aactccggct ccgccgctgc cggccgtggc cgccgtggcg cctccccctc
     9961 ccgcggctgc cgtttcggta ccggttccac ccccgccggc cccggagccc atcccgtctc
    10021 ctccgcccgt cctgacgacg ccctccccgg ccggaccctt tgccggagtg ggggagcgcc
    10081 tccgcgcgct gtcggcccag cgggaggccg gggcggcgaa gccggcggcc agcctggagg
    10141 agcggctcgg cgcgcggctg ccggtgtgga tcggctccat cgccctggct ctcgcggggg
    10201 cgttcctggt caagtactcg ttcgaccagg gtttcctgtc gccggtggtg cgggtggcgc
    10261 tgggggtggc gttcggcgtc gcgctgctgg cggggggcga gtggatgcgg cgctcgtcgc
    10321 cggggatctc gcaggggctg tcggccgccg gcatcgccga cctcttcgcc tgcttcctcg
    10381 ccggcgtcca cctctaccag ctcatctcgc cggcggtcgg cttcgccttc atggccctga
    10441 cggcgctcgt cgccgtgctg ctctccctgc ggcacggccc gatggtggcg ctcatcggcc
    10501 tggcgggcgg gttcctgacc ccggcgctga tccgggccgg ggagccggac gtccgcaacc
    10561 tcttcgccta cctgctcgtc ctcgtgatcg cggtggccgt cgtctcgggg caacgcggct
    10621 ggcgctgggt gaccgggctc gccctggccg gcggcatcgt ctgggtgctg gtctggctgg
    10681 cggggccgtt ccagaccgac gacgccccct ggctcagcct cttcctggtg gcgctgtcgg
    10741 tggccgcggt ggtctcggac ctccggcgcc gcgcgctcgc tccggacgga gccggcgggg
    10801 tgcccggagg gacctggctc tcctggggag ccctggtggc gtcgctcggc gtcatcgcca
    10861 tagtgacggg ggcgagcggc tactcgacgc tggagtgggc cttcctcggg ctcctggccg
    10921 gcagcatcct gatcctcgcc cgtctccaca cttccttcga ggggctcggc tgggtggcgg
    10981 ctgcggcccc ggcgctgctc ctcgccgcct gggggtacga tctgcccgat gtcggcagca
    11041 gcggcgccgg ccgcttcctg cgcacggtcc tcgccttcgg cgccctcttc gccatcggcg
    11101 gctacctggc gcaccgcggc gcgccccggc cgggccgctg ggcgtcgctc tcggcggcgt
    11161 cggcggtcgt ctacttcctg ctcgcctggg ccacgacggt ccaggacgtg gacctcccct
    11221 ggggcggcct cgcgctcggc cttgcggcgc tctacatcgt ggcgctcctg ccggtcgccc
    11281 ggcagcgacg cgaccacccc cacctcgacg ccccccttgc cgcgctggcc gtggcggtca
    11341 ccgtcttcgt cagcctggcg gttcccctcg agctggagcg ccagtggctg accgtggcct
    11401 gggcgctcga ggtcgcggct ctgacctggc tggccggccg cttccggctg ccggcgctca
    11461 ccctgctcgc ccgtctgctg gcgctggcgg tcctggtccg cctcctcctc aatcccgagg
    11521 tcttcaacta cccgatcggc gagcacccgg tcttcaactg gatcgtctac ggctacggcc
    11581 tgccgctcct cgccttcatc gccgccgccg tcctggcccg tcgccaaggt cgcacccagc
    11641 cggcgaccga gctggagtgg ggggccctgg cgatcaccct cgtcttctcc accctctggg
    11701 tccgccagct cttccatcct ggggaaccgg ggcgtgtcga gaccggcttc gtggagctcg
    11761 gcaccctcgc tgccctctgg atcgccctcg gcctcctcat cctcgtcctc agccgcaggg
    11821 tgccccagct cctcccgagc ggaggggagg ggcgcccggc gcaggcgact ggcagcgtgg
    11881 ggggagggga gggcgggagc gcacacgaag gagccgagca gggcgcccct cccctcctgg
    11941 cgagcctcga atcgggcggg cgggccttcc tcctcctggg ctgcctgacc gccctcttcg
    12001 gcgccggctt cctccagaac ccgctctgga cccacgagcc cgccggctcg ctcccaatct
    12061 tcaacctcct cctctgggcc ttcggagcgc cggccgccct cctcgccctg ggggcccgcg
    12121 agctgtcccg ccgcgcctcg cgcctcccgg ccgtcctgac catcgccgcc ctggcgctca
    12181 cgttcctcct ggtctccctc gaggtccgcc aggccttcca gggcacctac ctggacaccg
    12241 gcgtcacctc ggcggccgag cgctacacct actcggcggc ctggatcgcc ttcggcatcg
    12301 ccctcctggt catcggcacc gccaggaagg gcaaggcctt gcgctacgcc tccctggcgg
    12361 tgatgctgct caccgtgggc aaggtgttcc tctacgacac ggcgaacct
//
LOCUS       DASPJI010000200         4865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_413529, whole genome shotgun sequence.
ACCESSION   DASPJI010000200 DASPJI010000000
VERSION     DASPJI010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413529"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VEL74_05275"
     CDS             <1..382
                     /locus_tag="VEL74_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HYG61969.1"
                     /translation="TRLLTNQGATVAVAGGPRERALGERLAATSPRVRNLAGHLSLRE
                     SFALLAAADLVVCNSSVWMHAAAAFSRPTFVLLGESFPSARQHDAQWGYPGTCWSLGK
                     EEGGRAALYTPEEALARIRAEGRL"
     gene            382..1431
                     /locus_tag="VEL74_05280"
     CDS             382..1431
                     /locus_tag="VEL74_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006101440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYG61970.1"
                     /translation="MNLVHIVPTLPPPEEGVGTYALALARALARGHGISSGFVVGNPD
                     WPGRGEAEGFPVSRLPARSAAELVSSLTRAGTATVLVHYVNYGYQRRGCPYWLVDGLT
                     RWRRERPRHRLVTVFHEVYATGLPWQSSFWLQPVQRRLARALLRRSHGAVTSLDLYVE
                     RLGGETAMGGIAVSPVFSTVGEPRTVPPLADRAPRLVVFGGRGVRLRTWTGRLAELEA
                     ACRALGIEEICDVGPAVDSPATVAGVPVLRYGIRSVPEVSELLGGSRAGFLAYPPDFL
                     AKSTIFAAYCAHGLVPVCAWTPPLPARETALERLFWRPGTSETPENIARAARTWYEGH
                     SLNRQTETFRRLVSP"
     gene            1428..2501
                     /locus_tag="VEL74_05285"
     CDS             1428..2501
                     /locus_tag="VEL74_05285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYG61971.1"
                     /translation="MRLLLYSPAFLPDVGGLEIAVATMAGEFHRAGHEVVVVCQVASP
                     KPDDFPYRVVRRPGARELLRWTRWCDIYCQFNVSLRGLWPLLLVRRPWAVSHHSWYRQ
                     PDGRVAWQDRLKRYLLRFAASSISVSQAIADDLGTPSVVIGNAYRDDLFRRIPPPPEA
                     ERDRELVFVGRLVSDKGVDILLDAMALLAREGLALRLAIAGDGPEMTRLRGQAARLGI
                     GSRVGFLGTRTGEDLVQLLNRHRVLVVPSRYNEPFGIVALEGIACGCAVIGSEGGGLK
                     DAIGPCGRTFSNGDPVDLARVLREVFEDPETPAQFQRQAPEHLAAHTSAGVAAEYLAE
                     LENALTARGDTRSSGPATREARG"
     gene            2498..3937
                     /locus_tag="VEL74_05290"
     CDS             2498..3937
                     /locus_tag="VEL74_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61972.1"
                     /translation="MNTRLLFMAAGAAVLVWCFRNWRQAIKMALLLVILEGAIRKWLL
                     PSAQDLVYFAKDAVLLAAYCGYIGAGGRAQRLKGRPPQILILVLGFAAVLGAMQVFNP
                     HLPSLLVGFLGFKSYFLYVPLLVVLPAVFPDSASLAHFLRRYVLIAIPVGLLATAQFF
                     SPPSSFLNTYARGGIETATTFGSSNFVRVTGTFSYITGFSSYLLCTAILILGLLASTG
                     WTLRRQLPIYAALALTVMGMLMTGSRGPVYSLALVFPFYWLLAMRERGSGGMVTRIAL
                     GVGLIAAFITYSAPEAAEAFRGRASSAADVSGRMLAPFLAPLDSFQWGGLLGYGIGAT
                     HQAAATLAGSAVPFWTQGITVEAESGKVMLELGPVGFLLVYFARIFLIFFAFQQALRL
                     RTRFHRTLATACFLFFLASLPGYVVFDPTAGVYYWFFAGLLTLAVRLDRQALAAARAA
                     AQPVAEPAPQAVPVLTRNAWQLPNVPRSS"
     gene            3904..>4865
                     /locus_tag="VEL74_05295"
     CDS             3904..>4865
                     /locus_tag="VEL74_05295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYG61973.1"
                     /translation="MAASERPQVLVTHPGRQHSHQAALGLQEAGMLAGYWAGVPALER
                     HARGIPRVLWRRLIRYAPVPLEERRVRWVPMVPALRQLGGPLPAGPAAEVDLLACRLF
                     DRWAARRLRSSGAGAVIACEISALALFREAKRLGMTTLLDAPSLHHLVQDRLHGTTDP
                     PGLHRRIARVKDEEIALADHVLTVSELARQTYLEAGVPAEKVHAVPLGADLDLFAPDE
                     TRTAPEGLTFLFAGASIRRKGFDLLLAAFARVAADVPEARLRLVGPRGDSAHLLERYP
                     EAPISVAGPTDQRGLASELRRADCLVLPSRNDSYGMVVAEALASG"
BASE COUNT          582 a         1806 c         1692 g          785 t
ORIGIN      
        1 gacacggctg ctcacgaacc agggcgccac ggtcgccgtg gccggcggcc ccagggagcg
       61 ggctctcggc gagcgcctgg ccgccacctc cccacgggtc cgcaacctcg cggggcatct
      121 ctcgttgcgg gagagcttcg cgctcctcgc cgcggccgat ctggtggtct gcaattccag
      181 cgtctggatg cacgccgctg cggcgttctc gcgccccacc ttcgtgctgc tgggcgagtc
      241 gttcccctcg gcacgccagc acgacgccca gtggggctac ccgggcacct gctggagcct
      301 gggcaaggag gagggcgggc gggcggctct ctacactccg gaggaggcgc tggcccggat
      361 ccgggccgag gggcggctct aatgaacctc gtgcacatcg tcccgacgct gccgccgccc
      421 gaggaggggg tcggtaccta tgctctggcc ctggcgcggg cattggccag agggcacggc
      481 atctccagcg gcttcgtcgt gggtaatccc gattggccgg gccgcgggga ggccgagggc
      541 ttcccggtgt cgaggctccc ggcccggagc gccgccgagt tggtctccag tctcaccagg
      601 gcgggcacgg ccaccgtgct ggtccactac gtcaactacg gctaccagcg ccgcggctgc
      661 ccatactggc tcgtggacgg tctgacgcgc tggcgcagag agcgcccccg ccaccggctg
      721 gtgacggtgt tccacgaggt ctacgcgacg ggcctcccct ggcagagctc cttctggctt
      781 cagccagtgc agcggcggct ggcccgggcg ctgctccgcc ggagccatgg cgccgtgacg
      841 agcctggacc tctatgtgga acggctggga ggagagaccg cgatgggagg gatcgcggtg
      901 tcgccggtct tctccaccgt gggggagccg cggaccgtac cccctctggc ggaccgggcg
      961 ccgcggctgg tggtcttcgg cggccgcggc gtgcggctgc gcacctggac cggccggctg
     1021 gccgagctgg aggcggcctg ccgggcgctg gggatcgagg agatctgcga cgtcggcccc
     1081 gccgtcgatt ccccggccac ggtggccggc gtaccggtgc tccggtatgg aatccgctcc
     1141 gtcccggagg tgagcgagct gctggggggc tcccgggcgg gcttcctggc ctatcctccg
     1201 gatttcctgg cgaagtccac catctttgcc gcctactgcg cccacggcct ggtgccggtc
     1261 tgcgcctgga cgccgccgct gccggcgcgc gagaccgccc tggagcggct cttctggcgg
     1321 cccgggacgt ccgaaacacc cgaaaacatc gcccgcgcgg cccgaacgtg gtatgaaggg
     1381 cactccctga accgacagac cgagacgttc cgcaggctgg tgtcaccgtg aggcttcttc
     1441 tgtactcccc cgccttcctc cccgatgtgg ggggattgga gatcgccgtc gcgaccatgg
     1501 cgggcgaatt ccatcgcgca ggtcacgagg tggtggtcgt ctgccaggta gcgagcccca
     1561 agccggacga cttcccctac cgggtggtcc ggcggcccgg cgcccgggag ctgctgcgct
     1621 ggacccgctg gtgcgacatc tattgccagt tcaacgtcag cctgcggggg ctctggcccc
     1681 tcctcctcgt gcgccggccc tgggcggtct cccaccacag ctggtaccgg cagccggacg
     1741 gccgcgtcgc ctggcaggac cggctcaagc gctacctgct gcgcttcgcc gcctcctcca
     1801 tctcggtgag ccaggccatc gccgacgacc tgggcacgcc ctcggtggtg atcggcaacg
     1861 cctaccggga cgacctgttc cgccggatcc cgccccctcc ggaagcggag cgggaccgcg
     1921 agctcgtctt cgtcggccgg ctggtctcgg acaagggggt ggacattctc ctcgacgcca
     1981 tggccctgct ggcccgggaa gggctggcac tgcggctcgc catcgccggc gacggccccg
     2041 agatgacacg cctgcgtgga caggcggcgc ggctggggat cggctctcgg gtgggcttcc
     2101 tggggacgcg caccggggag gatctggtcc agttgctcaa ccgccaccgc gtcctggtgg
     2161 tcccctcgcg ctacaacgag ccgtttggca tcgtggccct ggaggggatc gcctgcggct
     2221 gcgccgtgat cggctcggag ggcggagggc tcaaggacgc catcggtccc tgcggacgga
     2281 cgttcagcaa cggcgatccg gtggacctgg cgcgcgtcct ccgcgaggtc ttcgaagacc
     2341 cggagacccc ggcccagttc cagcggcagg cccctgagca cctggcggct cacaccagcg
     2401 ccggagtggc ggccgagtac ctggcggagc tggagaacgc tctcacggcg cggggagaca
     2461 cgagatcctc gggcccggcc acccgggagg cccgcggatg aatacgcgcc tcctcttcat
     2521 ggccgcggga gccgcggtcc tggtctggtg cttccgcaac tggcgtcagg ccatcaagat
     2581 ggccctgctg ctcgtcatcc tggagggcgc catccgcaag tggctcctcc ccagcgccca
     2641 ggacctcgtc tacttcgcca aggacgccgt gctgctggcg gcctactgcg gctatatcgg
     2701 agcaggcggc cgcgcgcaga ggctcaaggg caggccgcca cagatcctga tactggtcct
     2761 ggggttcgcc gccgtcctcg gggcgatgca agtcttcaac ccgcacctcc ccagtctgct
     2821 ggtggggttc ctcgggttca agtcctactt cctctacgtg ccgctgctcg tcgtcctccc
     2881 ggcggtcttc cccgacagcg cgtcgctggc ccacttcctg cggcgctacg tgctgatcgc
     2941 gatcccggtg gggctcctgg cgacggccca gttcttcagc ccgcccagca gcttcctcaa
     3001 cacctatgcc cggggcggca tcgagaccgc caccacgttc ggaagctcca acttcgtacg
     3061 ggtgacgggc accttctcgt acatcacggg attctccagc tacctgctgt gcaccgccat
     3121 cctgatcctg gggctcctgg cgtccaccgg ctggacgctc cgccggcagt tgccgatcta
     3181 cgccgccctg gcgctgaccg tcatgggcat gctgatgacc ggctcgcgcg ggccggtcta
     3241 ctcgctggcg ctggtcttcc ccttctactg gctgctggcc atgcgcgagc ggggcagcgg
     3301 cggcatggtg acccgtatcg ccctgggcgt cggtctgatc gccgccttca tcacctactc
     3361 ggcaccggag gcggcggagg cgttccgcgg ccgggcgtcc agcgccgcgg acgtgagcgg
     3421 acgcatgctg gcgccgttcc tggcgccctt ggacagcttc cagtggggcg ggctcctcgg
     3481 ctacgggatc ggtgccaccc accaggccgc cgccaccctc gccgggagcg ccgtgccgtt
     3541 ctggacgcag ggcatcacgg tcgaggcgga gagcggcaag gtgatgctgg agctggggcc
     3601 ggtcggcttc ctcctcgtct acttcgcccg catcttcctg atcttcttcg ccttccagca
     3661 ggcgctgcgg ctacgcaccc gcttccaccg caccctcgcc acggcctgct tcctcttctt
     3721 cctggcctcg ttgcccggct acgtagtctt cgaccccacc gccggcgtct attactggtt
     3781 cttcgccggc ctcctcacac tggcggtccg tctcgaccgc caggcgttgg cggcggcccg
     3841 cgccgccgct caacccgtcg cggagccggc gccccaggcc gtccccgtcc tgacccgaaa
     3901 cgcatggcag cttccgaacg tccccaggtc ctcgtgaccc atcccgggcg ccagcactcg
     3961 caccaggccg ccctggggct ccaggaggcc gggatgctgg cgggctactg ggcgggcgtc
     4021 cccgcgctgg agcgccatgc ccggggcatc ccgcgcgtcc tgtggcggcg gctcatccgc
     4081 tacgcgccgg tgccgctgga ggagcggcgg gtgcggtggg tcccgatggt gccggccctg
     4141 cggcagctcg gcggcccgct gccggcagga cccgcggccg aggtggacct ccttgcctgc
     4201 cggctgttcg accgctgggc ggcgcgacgg ctgcggagct ccggggccgg cgccgtcatc
     4261 gcctgcgaga tctcggcgct cgccctcttc cgggaggcca agcggctggg gatgacgacg
     4321 ctgctcgacg cgccgtccct ccatcacctg gtccaggacc ggctgcacgg caccaccgac
     4381 ccgccgggtc tccaccgccg gatcgcccgc gtcaaggacg aggagatcgc tctcgccgat
     4441 cacgtcctga cggtgtcgga gctcgcccgc cagacctatc tggaggccgg cgtgccggcc
     4501 gagaaggtcc acgccgtgcc tctcggggcg gacctcgacc tcttcgcacc ggacgaaacc
     4561 cgcacggctc cggaggggct caccttcctc ttcgccggcg cgagcatccg ccgcaagggg
     4621 ttcgacctgc tcctcgccgc cttcgcccgg gtcgcggccg acgtgccgga ggcccggctg
     4681 cggctcgtcg gtccgcgggg cgactcggcc catctgctgg agcgctatcc ggaggctccg
     4741 atctcggtgg ccggccccac cgaccagcgg ggactcgcct ccgagctgcg ccgggccgac
     4801 tgcctggtcc tgccgtcgcg caacgactcc tatgggatgg tggtggccga agcgctggcc
     4861 tccgg
//
LOCUS       DASPJI010000201         4720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_72313, whole genome shotgun sequence.
ACCESSION   DASPJI010000201 DASPJI010000000
VERSION     DASPJI010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72313"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4720)
                     /locus_tag="VEL74_05300"
     CDS             complement(<1..>4720)
                     /locus_tag="VEL74_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG61974.1"
                     /translation="PVRLSGELGQEVRALSRREGATPFMVLLAAFQALLSRYSGQVDL
                     AVGTPIAGRNRLETEGLIGFFVNTLALRGDVSGEPSFRALLARTRETSLAAHTHQDVP
                     FERLVDELAPERNLSHTPLFQVVLVLQNVPAAAPGFAGLTLAPLPVQTGTAKFDLTLT
                     LAERESGLEGQVEYATDLFDGATVDRLLEHWVRLLEAALAAPEARIGELPLLIVAERA
                     ALLAAWAPAAGYPEERTLPELFREQVARRGEAEALSFEDGRLIYRELGRRVRSLAHRL
                     RRLEVGPEVRVGLCAERSVDLVVGLLGILEAGGVYVPLDPSYPADRLAFLMEDSAIGV
                     VAGQEAVRERLPAGPWAWVPLAQEATPEDSEAPLPPPLPDSAAYVIYTSGSTGRPKGV
                     VVTHRNVTRLLRSTEAVFGFGPGDVWTLFHSFAFDFSVWELWGALAYGGRLVVVPYWL
                     SRTPKAFLELLEREGVTVLNQTPSAFRQLTRIEQERPLALRVVIFGGEALEPSSLLGF
                     HRRYPGVRLVNMYGITETTVHVTFRELSDRDLEGASSVIGRAISDLGLYVLDRHREPV
                     PVGVGGELHVGGAGLARGYLGRPELTAERFVPDPFASSRGEAGARLYRSGDLGRYVAS
                     GDLEYLGRIDEQVKIRGFRIELGEIETALSSHPGVREAVVVARPPASGGGGDRRLVGY
                     AVGRPGGALDLGALRAYLRERLPEHMVPVLIELPALPLTINGKVDRKALPEPETQRVQ
                     VEQRPPQTWTEEVLAGIWSRVLGLGEIGTGESFFALGGDSILSLQVVALARERGLSLE
                     LQDLFQHQTLRDLAQAVDRNGAAGAADLVRTGPFSLVSPADRTLLPDDLEDAYPLSQL
                     QGGMLYHMQLAAEEAPYHNVDTWLLRARFDAPLFEQAVARVVARHAVLRTSFEQTRYS
                     ESLQLVHREARLPVMVEDLRGLPVPEQQERIATLFASEKERRFDLGRAPQLRFAVQVL
                     SGDSFQFTLTENHAIFDGWSLHTALSEIFSGYFALQAGRELPEEPPPAVTFRDFIALE
                     RQALASEEHLRFWDQQMRGWTFTELPPADPGAPEQSLSRKLEVAVPAEVWTGLQGLAR
                     ELAVPVKSVLLATHLKAIGRVSGSDDVLIGLTSNGRPETSDGEKVCGLFLNLVPFRLV
                     LEPGTWHDLVREVFARERAILPFRRFPMPALQRRLGGQKLFEIAFNYIHFHVVDALVR
                     SGDMQVLGFKGSETTNFAWQAHFTQIPTTASLALMFEHDLQRVSHRQAAAIAEVYLRI
                     LTAMAADGAGRNDGPLLSEAERQQTVVEWNDSATGGEPLPLVHELIARHSRHTPEALA
                     VAHGDETLSYGELDVRARRLARRLAELGVGPEVRVGLLLERSIELVVGLLSILEAGGA
                     FVPLDPAYPRERLAFSLRDSGASLLLTRRALLESLPGVEGVTPLYLDEPTKEITEPAY
                     PAPGPGVGQDNLAYVLYTSGSTGRPKGVMVPHRGLSHYLDWCLQAYPVAEGWGAPVHS
                     SIAFDLTITSLFAPLTAGRSIRLLDESLAAEALADALSEASQGERYSLVKLTPGHMTL
                     LADRLQGGDAAGALGLVIGGE"
BASE COUNT          786 a         1732 c         1561 g          641 t
ORIGIN      
        1 cctcgccgcc gatcaccaga ccgagagcgc ccgccgcgtc tcctccctgg agacgatcgg
       61 cgagcagcgt catgtggccc ggagtaagct tgaccagact gtagcgctcg ccctgggacg
      121 cctcggagag cgcatcggcc agcgcctcgg ccgccaacga ctcgtccagc agccggatgc
      181 tccttcctgc cgtcagaggc gcgaaaaggc tggtgatggt gaggtcgaac gcgatcgacg
      241 aatggaccgg cgctccccac ccttcggcca cgggataggc ctggagacac cagtccaggt
      301 agtgagacag cccgcggtgg ggcaccatga cccccttcgg cctgccggtg gagcccgaag
      361 tgtagaggac gtaggccagg ttgtcctgcc cgaccccggg ccccggcgcc gggtacgcgg
      421 gctcagtgat ctctttcgtc ggctcgtcga ggtagagcgg cgtcaccccc tcgaccccgg
      481 ggagcgactc caggagggct ctccgggtga ggagcagaga ggcccccgaa tccctcaatg
      541 agaaggcgag ccgctcccgc gggtacgccg gatcgagagg cacgaaggcc cctcccgcct
      601 cgaggatcga gagaagcccg accaccagct cgatggagcg ctcgaggagc aggccgaccc
      661 ggacctcggg cccgacgccg agctccgcca acctgcgggc cagacgccga gcacgcacgt
      721 ccagctcgcc gtagctcagc gtctcgtcgc catgcgccac cgccagcgcc tccggtgtgt
      781 ggcgggagtg ccgggcgatc agctcatgga ccagggggag cggttctcca ccggtcgcgc
      841 tgtcgttcca ctccaccacc gtctgctgac gctcggcttc cgacaggaga ggcccatcgt
      901 tccggccggc accatcggcg gccatggcgg tcaggatccg cagatacacc tcggcgatgg
      961 ccgcggcctg gcggtggctc acgcgttgga ggtcatgctc gaacatcaga gccagcgaag
     1021 cggtggtggg gatctgggtg aagtgggcct gccacgcgaa gttggtggtc tccgatccct
     1081 tgaagcccag gacctgcatg tcgcccgagc gcaccagcgc gtcgacgacg tggaagtgga
     1141 tgtaattgaa ggcgatctcg aacagcttct ggccgccgag acgccgctgc agtgccggca
     1201 tggggaagcg ccggaagggg aggatggcgc gctcgcgggc gaagacctcg cggacgaggt
     1261 cgtgccaggt tccgggctcc agcaccagac ggaatggcac gaggttcagg aagaggccgc
     1321 agaccttctc gccgtcggag gtctcggggc ggccgttgct ggtcaggccg atgaggacgt
     1381 cgtcgctgcc gctcacccgg ccgattgcct tgagatgagt ggcgagcagc acgctcttga
     1441 ccggcaccgc cagctcccgc gccaacccct ggagcccggt ccagacctcg gccgggaccg
     1501 ccacttccag cttgcgcgag agcgactgct ccggtgcccc gggatcggcc ggcggcagct
     1561 cggtgaaggt ccagccccgc atctgctggt cccagaagcg caggtgctcc tccgaggcca
     1621 gagcctgccg ctccagggcg atgaagtcac ggaacgtcac cgccggcggc ggctcctccg
     1681 gcagctcccg gcctgcctgc agcgcgaagt acccggagaa gatctccgac agcgctgtgt
     1741 gcaggctcca gccgtcgaag atcgcgtggt tctcggtgag ggtgaactgg aacgaatccc
     1801 cgctcagcac ctgcaccgcg aaacggagtt gcggggcccg gccgaggtca aagcggcgct
     1861 ccttctcgct ggcgaaaagc gtcgcgatcc gctcctgctg ctctggcacc ggcaaccctc
     1921 tgaggtcctc gaccatcacc ggcaggcgcg cctcgcggtg cacgagctgt aacgactcgc
     1981 tgtaccgggt ctgctcgaac gaggtacgca gcacagcgtg ccgggccacg acgcgggcga
     2041 ccgcctgctc gaagaggggg gcgtcgaagc gtgcccgcag gagccaggtg tcgacgttgt
     2101 ggtacggcgc ctcctccgcg gccaattgca tgtggtagag catgccgccc tggagctggg
     2161 acagcggata ggcgtcctcc agatcgtcgg ggagcagcgt ccggtccgcc ggcgaaacca
     2221 gggagaacgg ccccgtacgg acgaggtccg cggccccggc cgccccgttg cggtcgacag
     2281 cctgtgcaag gtcgcggagg gtctggtgct ggaacaggtc ctggagctcc agcgagagcc
     2341 ctcgctcccg cgccagcgcc accacctgga ggctgaggat cgagtcgccg cccagagcaa
     2401 agaagctctc cccggtcccg atctcgccaa ggcccaggac ccgcgaccag atccccgcca
     2461 gcacctcctc ggtccaggtc tggggaggcc gctgctccac ctgcacccgc tgcgtctccg
     2521 gctcgggcag cgccttgcgg tccaccttgc cattgatcgt cagcggcaac gccggcagct
     2581 cgatcaacac gggcaccatg tgctccggca gccgctcccg gaggtatgcg cggagcgctc
     2641 ccaggtccag agccccgcct ggacgcccca cggcataacc gacaagacgc cggtctcctc
     2701 caccgcccga ggctggtggc cgtgccacta ccacggcctc gcgcaccccc ggatggctcg
     2761 acagcgccgt ctcgatctcc ccgagctcga tgcggaaacc gcggatcttc acctgctcgt
     2821 cgatgcggcc caggtactcc aagtcgccgc ttgccacata ccggcccagg tcgcccgagc
     2881 ggtagagacg tgccccggcc tctcctctgg agctggcgaa aggatcaggc acgaaacgct
     2941 ccgccgtcag ctccggacgc cccagatacc cgcgcgccag acccgcgccg cccacgtgga
     3001 gctctccccc taccccgacc ggaaccggct cgcggtggcg atccagcacg tacagcccca
     3061 ggtcgctgat ggcccggccg atcacactgc tcgcgccctc caggtcccga tcggacaact
     3121 cccggaacgt cacatgtacc gtggtctcgg tgatgccgta catgttgacc aggcgcactc
     3181 ctggatagcg ccggtggaac ccgagcaggc tcgacggttc cagcgcctcg ccaccgaaga
     3241 tcaccacccg caacgcgagc ggtctttcct gttcgatccg cgtcagttgc cggaacgccg
     3301 acggcgtctg gttgagcaca gtgacgccct ctcgttccag cagctccagg aacgccttgg
     3361 gcgtccggct caaccagtac ggcaccacca ccagacggcc gccgtacgcc agcgcccccc
     3421 acagctccca gaccgagaag tcgaaggcga aggagtggaa cagcgtccag acgtctcctg
     3481 gaccgaagcc gaaaaccgcc tccgtagagc gcagcagccg tgtcacattg cggtgggtca
     3541 ccaccacgcc cttgggccgg cccgtggaac ccgaggtata gatcacatag gccgccgaat
     3601 cgggcagcgg tgggggcagg ggggcttcgg agtcctcggg cgtagcctcc tgcgcgagcg
     3661 gtacccaggc ccacggaccg gcgggcagcc gctcccgcac cgcctcctgt ccggccacga
     3721 cgccgatcgc ggagtcttcc atcagaaagg ccaggcgatc cgccgggtac gacggatcga
     3781 gcggcacgta aacgcccccg gcctcgagga tgcccaggag gcccaccacc aggtccacgg
     3841 aacgttcggc gcagagcccc acccgcacct cgggacccac ttcgagccgg cgcaggcggt
     3901 gggccagcga ccggacccgc cggcccaatt cgcggtagat caaccgcccg tcttcgaacg
     3961 acagggcctc ggcttcgccg cgccgcgcca cctgctcgcg gaacagctcc ggcagcgtcc
     4021 gctcctccgg atagcccgcc gcgggagccc acgccgccag cagcgccgcg cgctcggcga
     4081 caatgaggag cggcagctct ccgatccggg cctccggcgc cgccagggcc gcttcgagca
     4141 gccggaccca gtgctccaac agccggtcca ccgtcgcccc gtcgaagaga tcggtggcat
     4201 actcgacctg gccttcgagc ccggattccc gctccgccag cgtcagggtc agatcgaact
     4261 tcgccgtccc ggtctgcacc ggaagcggcg cgagcgtcag gccggcaaag ccgggcgccg
     4321 cggcgggaac gttctgcagc accaggacca cctggaacag cggcgtgtgg ctcaggttgc
     4381 gctccggcgc cagctcgtcc accagcctct cgaacggcac gtcctgatgg gtgtgcgccg
     4441 ccagcgacgt ctcccgcgta cgcgccagca gtgcgcggaa cgacggctcc cccgagacgt
     4501 cgccgcgcaa ggccagggtg ttgacgaaga acccgatcaa cccttcggtc tcgagacgat
     4561 tgcggcccgc gatgggtgtg cccaccgcca ggtccacctg gccgctgtag cgtgagagga
     4621 gcgcctggaa cgcggccagc agcaccatga acggtgtcgc gccctcccgg cggctgagcg
     4681 cccgcacctc ctgccccagc tcaccggata gccggaccgg
//
LOCUS       DASPJI010000202         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_185602, whole genome shotgun sequence.
ACCESSION   DASPJI010000202 DASPJI010000000
VERSION     DASPJI010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185602"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VEL74_05305"
     CDS             complement(<1..1041)
                     /locus_tag="VEL74_05305"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HYG61975.1"
                     /translation="MRSIQAWLLCALLGFSASALPAQEPQEGQTEEEGTAAPQTVEER
                     LDEIDQRLRVLDRRTELEKEQAAEKAKTATSVTSGRDGFSFKSNDGAFLLKVRGYVQL
                     DGRFWQGDEERPATDTFLLRRVRPIFEGTLYKNFDFRIMPDFGGGTTVLQDAYIDGRF
                     SPAAKVRVGKFKAPVGLERLQSGTDILFVERALPTNLVPNRDLGIQLFGDFAGGGVTY
                     AVGVFNGVPDGGTADSDTNDSKDVAARLFFQPFLAGGGAFKNLGVGIAASQGSQEGTL
                     TAPGLAGYRTPGQQTFFSYRSDGTATGTVIADGDRFRLSPQAYFYYGPFGLLTEYVQS
                     THEVRRGTVQEEL"
     gene            complement(1042..2967)
                     /locus_tag="VEL74_05310"
     CDS             complement(1042..2967)
                     /locus_tag="VEL74_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYG61976.1"
                     /translation="MSIVLEDLTKLYAGHPVVNRVSLEVAEGEFFVLLGSSGSGKSTI
                     LRMIAGLAEIDGGRVMLRGREVSDLPPAKRGVGFVFQNYALFRGMSVAENVEFALDIR
                     GTFKTERRRRRDELLELVGLAGLGGRMPRQLSGGQQQRVALARALAHRPEVLLLDEPF
                     GALDAKVRLDLRRTIREIQRELGITAIFVTHDQEEAFELADRLGVLSFGRLLEVGPPE
                     ELYLRPETEFVATFLGTANLMVGESTSVGVQLGPVQFPLTTRMEADGSRRVQVLFRPE
                     DVAVRDTADDFACPVLGEAVVDQRSFIGSFERLRLRLPPLSGVRTIAPAVPFGSDTIW
                     IEASRSQDQARRFPLRPGDSTFVGVQRVHALVHPGMRFLMLTDGTPASRSALALGGQL
                     ARLAHSRVTVLACGLRDGQADSHLQEVREQLGSGLAALDTRSTPLPPDAAAAEEAVRL
                     PSDLVMLGIPAGAPRDALAQAERVLAAGGHHLLLVPGATGGGTSSGAPHIPTRVLICV
                     AVGEPGKEDVAFAGRLVRHLGAEATVMTVLPEAERAPQGQTRPAAAQAERFLTSSART
                     LWRQGVPASTAVRYGPVREQILAQVTEGGHDLLVLGAPLPDRDGDISLAGLPARVLPE
                     LGQCPVLIVRSRTTERT"
     gene            complement(3097..>3603)
                     /locus_tag="VEL74_05315"
     CDS             complement(3097..>3603)
                     /locus_tag="VEL74_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYG61977.1"
                     /translation="TNRDLDAMLAEGSFRTDLYHRIADWKVTLPPLRQRKADIPNLAA
                     FFLAGEAERRGVAIAGISKGALALLEAYDWPGNIRQLQREMIRGGLFLEDGQLLETSH
                     LDDAVRQGRRTRPQGLRERLEEIERQEIQRALAAAGADVSAAATALGIGRSTLYRRMI
                     ELGIERPA"
BASE COUNT          528 a         1238 c         1335 g          502 t
ORIGIN      
        1 cagctcttcc tgcacggtgc cacgccgcac ctcgtgggtg gactggacgt actcggtgag
       61 cagtccgaag gggccatagt agaagtacgc ctggggcgag agacggaagc ggtcgccgtc
      121 ggcgatgacc gtgccggtgg cggtgccgtc ggagcggtag gagaagaagg tctgctggcc
      181 gggcgtgcgg tagccggcga gccccggagc ggtcagcgtc ccctcctggc tcccctggct
      241 cgcggcgatg ccgacgccga ggttcttgaa cgcgccgcca ccggccagga agggctggaa
      301 gaagaggcgc gccgccacgt ccttggaatc gttggtgtcg ctgtcggcgg tgccgccgtc
      361 aggcacgccg ttgaagacgc cgacggcgta ggtcacgccg ccgcccgcga agtcgccgaa
      421 gagctggatg ccgaggtcgc ggttcggcac caggttggtg ggcagcgccc gctcgacgaa
      481 caggatgtcg gttccggact gcaggcgctc caggcccacc ggcgccttga acttgccgac
      541 gcgcaccttg gcggccggcg agaagcggcc gtcgatatag gcgtcctgga ggacggtggt
      601 gccgccgccg aagtccggca tgatccggaa gtcgaaattc ttgtacagcg tcccctcgaa
      661 gatcgggcgg acgcggcgca gcaggaacgt gtcggtggcg ggccgctcct cgtcaccctg
      721 ccagaagcgg ccgtcgagct ggacgtagcc gcggaccttc aggaggaagg cgccgtcgtt
      781 ggacttgaat gaaaagccgt cccgtcccga ggtcaccgag gtggcggtct tcgctttttc
      841 ggcagcctgc tctttttcga gctcggtgcg ccggtcgagc acccgcaggc gctgatcgat
      901 ctcgtccagg cgctcttcca cggtctgagg cgcagccgtg ccctcctcct ccgtctgtcc
      961 ctcctgcggc tcctgggccg gcagggccga ggccgaaaaa cccaataagg cgcagaggag
     1021 ccatgcttgt atggatctca ttcaggttct ctccgtggtg cgcgatcgga cgatgagcac
     1081 tgggcactgg cccaactcgg gaagcacgcg ggcgggcagg ccggccaggg agatgtcgcc
     1141 gtccctgtcc ggcagggggg cgccgaggac cagcaggtcg tgccccccct cggtgacctg
     1201 cgcgaggatc tgctcgcgca ccggaccgta gcgcaccgcc gtggaggcgg gcacgccctg
     1261 acgccagagt gtgcgggcgc tgctggtgag gaaccgctcc gcctgcgcgg cggccggccg
     1321 cgtctggccc tggggcgccc gctcggcttc gggcagcacg gtcatgaccg tggcctcggc
     1381 gccgaggtgg cgcaccaggc ggccggcgaa ggccacatcc tccttgccgg gctcgcccac
     1441 cgcgacgcag atcagcaccc gcgtcgggat gtgcggcgcg ccgctcgagg tgcccccgcc
     1501 ggtagcaccg gggaccagga gcaggtgatg accgcccgcg gcgaggaccc gctcggcctg
     1561 ggcgagggcg tcacgcggcg cgccggccgg aatgcccagc atcaccaggt cggacggcag
     1621 gcgcacggcc tcctcggcgg cggctgcatc cggcggcagc ggcgtggacc gggtatccag
     1681 ggccgcgagg ccgctgccga gctgctcgcg gacttcctgc aggtggctgt cggcctgacc
     1741 gtcccggagg ccgcaggcga ggaccgtcac ccgggagtgg gccaggcgcg cgagctgccc
     1801 gcccagggcc agcgccgagc gcgacgccgg cgtgccgtcg gtgagcatca ggaatcgcat
     1861 gccggggtgg accagggcgt ggacgcgctg gacaccgaca aacgtggagt cgcccggccg
     1921 cagggggaag cggcgcgcct ggtcctgcga ccggctggcc tcgatccaga tggtgtcgct
     1981 gccgaacggc accgccggcg cgatggtccg cactccggac agcggcggca ggcggaggcg
     2041 caggcgctcg aaggagccga tgaacgagcg ctggtccacg accgcctcgc cgagcaccgg
     2101 gcaggcgaag tcgtcggcgg tgtcgcgcac cgccacgtcc tcgggccgga agaggacctg
     2161 gacgcggcgc gagccgtccg cctccatgcg ggtggtgagc gggaactgca ccggcccgag
     2221 ctggacgccg acggacgtgc tctcccccac catgaggttg gccgtaccga ggaaggtggc
     2281 gacgaactcc gtttcggggc gcaggtagag ctcctcgggc ggccccacct ccagcaggcg
     2341 gccgaaggag aggacgccca gccggtcggc gagctcgaac gcctcctcct ggtcgtgggt
     2401 gacgaagatg gccgtgatcc ccagctcgcg ctggatctcg cggatggtgc gccgcaggtc
     2461 caggcgcacc ttggcgtcga gggcgccgaa cggctcgtcg aggaggagca cctcgggccg
     2521 gtgcgccagc gcccgggcga gcgccacccg ctgctgctgg ccaccggaga gctggcgcgg
     2581 catccggccg ccgaggccgg cgaggccgac cagttccagc agctcgtcgc gccggcgccg
     2641 ccgctcggtc ttgaaggtgc cgcggatgtc gagggcgaac tcgacgttct cggccaccga
     2701 catgccacgg aacagcgcgt agttctggaa cacgaagccg acgccgcgct tcgccggcgg
     2761 caggtccgag acctcgcggc cgcggagcat cacccgaccg ccgtcgatct cggcgaggcc
     2821 ggcgatcatg cgcaggatcg tgctcttgcc gctgccggac gaacccagga ggacgaaaaa
     2881 ctccccctcc gccacctcca gcgacacccg gttcaccacc gggtgcccgg cgtagagctt
     2941 ggtcaggtct tcgagaacga tcgacattgg gctgcgctcc tccccgctcc cgggggctcc
     3001 ggtcctgctg gagaggaaga ttaggcagcc gtggtcacag agtcagtcga aaggtggtga
     3061 caaaaccggc gggttttgtc accggggatg aggtggtcac gccgggcgtt cgatgcccag
     3121 ctcgatcatc cggcgataga gagtcgagcg gccgatgccc agggcggtgg cggctgcgct
     3181 cacgtcggct ccggccgcgg cgagcgcgcg ctggatctcc tgcctctcga tctcctccag
     3241 ccgctcccgg aggccctgcg ggcgcgtccg tctcccctgc cggaccgcgt cgtcgagatg
     3301 ggaggtctcc aacaactgac cgtcctccag gaagaggccg ccgcggatca tttcgcgctg
     3361 gagctggcgg atgttgcccg gccagtcgta cgcctcgagg agggccagcg ctcccttgga
     3421 aatgcccgcg atggccaccc cccgccgctc cgcctcgccg gccaggaaga aggcagcaag
     3481 gttggggatg tccgccttgc gctggcgcag cggcggcagc gtcaccttcc agtcggcgat
     3541 ccggtgatag aggtcggtgc ggaagctccc ctcggccagc atggcgtcca ggtcgcggtt
     3601 ggt
//
LOCUS       DASPJI010000203         7001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_697764, whole genome shotgun sequence.
ACCESSION   DASPJI010000203 DASPJI010000000
VERSION     DASPJI010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7001)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7001
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697764"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VEL74_05320"
     CDS             <1..383
                     /locus_tag="VEL74_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HYG61978.1"
                     /translation="DALRQDLSATAHLLIRLRVLHPLLSVTAGFLILYMALQLPEQRK
                     GEGIARYARWTMGVVLTQLMAGVVNVLLLAPVWMQLVHLLLADLLWIGFVLFSASVLA
                     ARGAEAGIQEPAGAVAMGIDKGSV"
     gene            complement(782..2389)
                     /locus_tag="VEL74_05325"
     CDS             complement(782..2389)
                     /locus_tag="VEL74_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61979.1"
                     /translation="MAASREEEERPGGRLLPGAAAALWTSLAALFTVRLAGMAPDDFF
                     VTYRYAWNLAGGNGFVFNPGERVFGLTDPGLGLLLAALHLVTRLPIPWLGTAVTGAAL
                     VGIALVLLLDGRERGRTPEALVGGTLIVGSSFLWVNQGGGVFFALLFLLIAARLRSPV
                     RAGLLAGLAVWMRPDAALGVGLLGLLLGIEKRRLPWTYLLAAGGVASAGVLAAWSWFG
                     SPLPNTLAAKHAMTVGMTEVGSGFRGFWPRAFRLMSRHWGPWWTLMTGVGLAGHAAML
                     LGGGGRGSAGPAQATGSEGGWEGASAHEGAEQGRTEPRGGGRATRLLALVSLAMALAY
                     PLLEVPFFSWYAVPTAVGVLYGVPYLTGALGRGLAARLGRRQMVGAAAATAILAPLLL
                     SFVPATWRWHTQYQWQRHLRTYREAALWIRDHSAPQDALAYVEIGVMSFYSERPLVDL
                     LGLVTPGAIPYVEADDLVGAFLAHPTRFVIHHSRGRMSPLIQRRWFPRAYEAVAYFHE
                     GDGRGLTIYQRRPGSKLPRPRPPRERR"
     gene            complement(2376..5120)
                     /locus_tag="VEL74_05330"
     CDS             complement(2376..5120)
                     /locus_tag="VEL74_05330"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HYG61980.1"
                     /translation="MNRFVKPIAASVLSLMIASVGAVSAQEARGPLAPQPLEPAGTGG
                     IATVERALAKLMTHKRLLVVAAHPDDEDTSLLTLIARGMGGEAAYLSLSRGEGGQNLI
                     GPELGVGLGLIRSRELLAARAVDGGRQFFTRAFDFGYTRSLDETLTLWPREVLLEDAV
                     RVIRRFKPQVIVSIFPGVPHPNHGQHQAAGVTAYEAFRLAGDPAALPRLRQEGLLPWK
                     PQALFRSTFFSPDATTLVLSTAPVDPITGKSIEQLAMASRSMHRSQDMGLFQQLGPDD
                     TRVGWVEGGAGAQSKDLFEGIDTRVTAIAAVIPEKYARRHAGEHLETVAVRAEQVRRS
                     LSPSRLSDAVPGLAEIRKNLRAVYHLVEDRVPEAERRPVLELIQEKIDAADLALAAAA
                     GVAVDAFTERETLIQGETFPVRAVLWNSGGGVVENVRVTLLPTADNEWGGGPIEPKPP
                     GQEPPPAPGTSPRAAPNPAIQVPPGGSVPAGQLAGYDFEPAVPAQAALTVPYFLRKPL
                     AGAGTTGSLYDWSDSPASVKGEPFGPPPLTARFSFTIDGAAVTLEREVVHRHRDQALG
                     EIRRPLRVVPRVEVQAAEDLLVWPVARREPRRLQVTVSSNAREAVRGRLELSAPAPWP
                     AVTPVPFALEPGGEHGLELVIPAPRGLAPGRYDLRLSAVLADGQRFDLAVPVLDYPHI
                     RPTPQPDPARLEVSAADLQLPPLKRVGYVRGASDRVPEFLSQAGVPLEMVGIRELEGD
                     LSRYDAIVVGSRAYETDPALARANPRLLDYARAGGLVIVQYQQYPFIQGKFAPLALDI
                     ARPHDRVTDETAAVRVLAPSHPLFTTPNRIGPEDWQGWIQERGLYFANTWDPAYVPLL
                     SMADPGGPELRGGLLVAPLGKGHYVYTGLAFFRQIPAGVPGAYRLFANLLALGGRGGK
                     "
     gene            complement(5161..6309)
                     /locus_tag="VEL74_05335"
     CDS             complement(5161..6309)
                     /locus_tag="VEL74_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61981.1"
                     /translation="MGDPFLTLAERQQTVDPGLLEEALALLAERGPEALARGPERLRG
                     PVAFEAFLRHSWAVLHADPAQAIESARWATRFGSRLDPEELGARGLADLRCRAWAILG
                     NAYRVADNLSAAEFAFTEAFNHFAAGSRDRSLQARLLDFQASFRADQRRFPEAIALLD
                     RVFAIFQERGDEHLAGRALLSKGLFTGYSGDPEGAIRLIEEGLSRIDSGRDPNLALWA
                     THNLSLFMIETGRFEEARALAHRNLRRFAEREHRIDFLKLRWIEARADAGLGLLDEAE
                     EAFVEVREGMLDVGKRYHSALAALDLAVIHRRQGRPEEACSTILEAAEMFLEIEISRE
                     AIAAVLALQDSCRAGLETGGLLEKVMDFLRHLERDPTLRFKDWAGQPC"
     gene            6379..6816
                     /locus_tag="VEL74_05340"
     CDS             6379..6816
                     /locus_tag="VEL74_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG61982.1"
                     /translation="MSEVQPDVQCDAEIQRILTVLRTSMRILGVTNREIEKRLGVSVG
                     YLSRLFHGSVELKVEHVLAITQAIGLHPAELFHLLYPQLPARPTEASLKLRSALEGFR
                     PAPPPSPAAPEKPGAHAPSEEDLERLMMSSLRKLLAGLGREPG"
BASE COUNT          992 a         2510 c         2590 g          909 t
ORIGIN      
        1 ccgatgccct gcgccaggac ctctccgcca ccgcccatct gctgatccgg ctgcgcgtcc
       61 tccacccgct cctctcggtg accgcggggt tcctgatcct ctacatggcg ctgcaactcc
      121 ccgagcagcg caagggggag gggatcgccc gctacgcccg ctggaccatg ggcgtggtcc
      181 tgacccagct catggcgggc gtggtgaacg tcctcctcct ggctccggtc tggatgcagc
      241 tcgtccatct cctcctcgcc gacctcctct ggatcggctt cgtcctcttc tccgcctccg
      301 tgctggcggc gcggggggcg gaggcgggga tccaggagcc cgccggagcc gtcgccatgg
      361 gcattgacaa ggggagtgtc tgaaatgccg cgtcagccgc cgactcctac gaagccttcg
      421 agcgggaggt cagccgcttc ttccaggaga ccaacgagcc cacggaggcg gagtggctcg
      481 aagcggtggc ggccccgtcg ccgcggatgc gggacggggc tcccgcagct catccggacg
      541 ccgtggatgg agtccggacg ccctggaagc ttcccgagcg ctgcggaagc ttcccggacg
      601 ctcgggatgg tgtccagctc aaaaggatgg aatccggagg ccgcggaagc ttcccgagcg
      661 cttcggatcg cgtccgggtc gtgcggaagg ctgccaattc aatcggatgg attccggagg
      721 ctccggaagc ttcccgaagg actcggatgg tgtccgatcc aaacggatgg agtcccggct
      781 cctaccgccg ctcccgtggc ggccgggggc gggggagctt cgagcccggg cggcgctggt
      841 agatggtgag cccgcggccg tccccctcgt ggaagtaggc gacggcctcg taggcgcggg
      901 ggaaccagcg gcgctggatg aggggggaca tccggccgcg gctgtggtgg atcacgaaac
      961 gggtggggtg ggccaggaag gcgccgacca ggtcgtcggc ctcgacgtac gggatcgccc
     1021 ccggcgttac cagtccgagg aggtccacca gcggcctctc gctgtagaag gacatcactc
     1081 cgatctccac gtaggcgaga gcgtcctgcg gcgccgagtg gtcccggatc cagagcgccg
     1141 cctcgcggta ggtccggagg tgccgctgcc actggtactg ggtgtgccac cgccaggtgg
     1201 ccggtacgaa cgagaggagg agcggcgcca ggatcgccgt cgcagcggcg gcacccacca
     1261 tctgtcgtct tcccagcctg gcggccagcc cgcgtcccag ggctcccgtg agatagggca
     1321 caccgtagag gactccgacc gccgtgggca ccgcgtacca ggagaagaag gggacttcga
     1381 gcagcgggta ggccagcgcc atggcgagcg agacgagggc caggagccgc gtggcccggc
     1441 cgcccccccg tggctcggtc cggccctgct cggctccttc gtgtgcgctc gccccctccc
     1501 acccgccctc gctgccagtc gcctgcgccg ggccggccga gccacggccc ccacccccga
     1561 ggagcatcgc cgcgtggccc gcgaggccca ccccggtcat cagcgtccac cagggacccc
     1621 agtggcgcga catcagccgg aaggcccggg gccagaaccc ccggaaaccg gagcccacct
     1681 ccgtcatccc caccgtcatg gcgtgcttgg cggccagcgt gttgggcagg gggctgccga
     1741 accaggacca ggccgccagc accccggcgc tcgccacgcc tcccgcggcc agcaggtagg
     1801 tccagggcag gcgccgcttc tcgatcccta ggagcaggcc gaggagaccc accccgagcg
     1861 ccgcgtccgg gcgcatccag accgcgaggc cggcgaggag ccccgcccgg accggcgagc
     1921 gcagacgtgc cgctatgaga aggaagagga gagcgaagaa gacgcctccc ccctggttga
     1981 cccagaggaa ggaggagccc acgatcagcg tcccgccgac gagagcctcg ggcgtccgcc
     2041 cccgctcccg gccgtcgagc agcagcacca gggcgatccc caccagggcg gcgccggtga
     2101 ccgccgtccc cagccagggg atgggcaggc gggtgacgag gtgcagggcc gcgaggagga
     2161 gacccaggcc gggatcggtc agcccgaaga cccgctcccc ggggttgaag acgaagccgt
     2221 tccccccggc gaggttccag gcgtagcgat aggtgacgaa gaagtcgtcc ggcgccatcc
     2281 cggccagccg tacggtgaag agagccgcga gcgacgtcca aagagccgcc gcggcccccg
     2341 ggaggaggcg tcctcccggc cgctcctctt cttctctact tgccgccacg cccacccagg
     2401 gccagcaggt tcgcgaacag acggtaggcg ccggggacgc ccgccgggat ctgccggaag
     2461 aaggccaggc cggtgtagac gtagtgaccc ttgcccaggg gcgccaccag caggcccccc
     2521 cgcagctcgg ggccgccggg gtccgccatc gacagcagcg gcacgtaggc cgggtcccag
     2581 gtgttggcga agtagagccc ccgctcctgg atccacccct gccagtcctc ggggccgatc
     2641 cggttcggcg tggtgaacag cgggtggctc ggggcgagca cccggaccgc cgcggtctcg
     2701 tcggtcaccc ggtcgtgggg acgggcgatg tccagcgcga ggggggcgaa cttgccctgg
     2761 atgaaggggt actgctggta ctggacgatc accagcccgc cggcccgggc gtagtccagg
     2821 aggcgcgggt tggcgcgcgc cagcgccggg tcggtctcgt aggcgcggct gccgacgacg
     2881 atggcgtcgt agcgggagag gtccccctcc agctcccgga tccccaccat ctccagcggc
     2941 acgccggcct ggctgaggaa ctcggggacc cggtccgagg cgccgcgcac gtagccgacc
     3001 cgcttcagcg gggggagctg gaggtcggcg gcgctcacct ccaggcgggc ggggtcgggc
     3061 tgcggcgtcg gacggatgtg cgggtagtcg aggaccggca ccgccaggtc gaagcgctgg
     3121 ccgtccgcga ggaccgcgga gagccgcagg tcgtagcggc cgggcgcgag accccggggg
     3181 gccgggatga ccagctccag cccgtgctcg ccccccggct ccagggcgaa cggcaccggc
     3241 gtcaccgccg gccagggcgc cggcgccgag agctccagac ggccgcgcac cgcctcgcgg
     3301 gcattggatg acacggtcac ctgcaggcgc cggggctcgc ggcgggccac cggccagacc
     3361 agcaggtcct ccgcggcctg gacctccacc cgcggcacca cccgcagcgg ccgccggatc
     3421 tcgccgagcg cctggtcgcg gtgacggtgg accacctccc tctccagggt gacggcggcg
     3481 ccgtcgatcg tgaaggagaa gcgggcggtg agggggggcg ggccgaaggg ctcgcctttg
     3541 accgaggctg gtgagtccga ccagtcgtag aggctccccg tcgtccccgc tccggccagg
     3601 ggcttgcgca ggaagtaggg gacggtgagc gccgcctggg cggggacggc gggctcgaag
     3661 tcgtagccgg cgagctggcc ggcgggcacg ctcccgccgg gcggcacctg gatcgcgggg
     3721 ttcggggccg cccggggcga cgtgcccggc gcgggcgggg gctcctggcc gggcggcttg
     3781 ggctcgatcg gcccgccccc ccactcgttg tcggcggtgg ggagcagggt gacgcgcacg
     3841 ttctccacca ccccgccgcc gctgttccag aggacggcgc ggaccgggaa ggtctccccc
     3901 tggatcagcg tctcgcgctc ggtgaaggca tcgaccgcca cgcccgcggc ggcggcgagc
     3961 gccaggtcgg cggcgtcgat cttctcctgg atcagctcca ggacggggcg ccgctccgcc
     4021 tcgggcacgc ggtcctccac caggtgatag accgcccgca ggtttttgcg gatctccgcc
     4081 aggccgggca cggcgtccga gagccgggag gggctcaggg accgccgcac ctgctcggcg
     4141 cgcacggcca ccgtctcgag gtgctccccg gcgtggcggc gcgcgtactt ctccgggatc
     4201 accgcggcga tggcggtcac gcgcgtgtcg atcccctcga agaggtcctt ggactgggcg
     4261 ccggcgcccc cctccaccca gccgacacgg gtgtcgtccg gcccgagctg ctggaagagg
     4321 cccatgtcct gcgagcggtg catgctgcgg ctggccatcg cgagctgctc gatggacttg
     4381 ccggtgatcg ggtcgaccgg agcggtggag aggacgaggg tggtggcgtc gggcgagaag
     4441 aaggtggagc ggaacagggc ctggggcttc cacggcagca gcccctcctg ccggagccgg
     4501 ggcagcgccg ccgggtcccc cgccagccgg aacgcctcgt aggcggtcac ccccgcggcc
     4561 tggtgctggc cgtggttggg gtgcggcacg ccggggaaga tcgagacgat cacctgcggc
     4621 ttgaagcggc ggatgacccg cacggcgtcc tccaggagca cctcgcgcgg ccagagcgtc
     4681 agggtctcgt cgagcgaccg ggtgtagccg aagtcgaagg cgcgggtgaa gaactgccgg
     4741 ccgccgtcga cggcgcgggc ggcgaggagc tcgcggctgc ggatgagccc caggcccact
     4801 cccagctccg gtccgatcag gttctgcccc ccctcgccgc ggctcagcga gaggtaggcc
     4861 gcctcgccgc ccatgccgcg ggcgatcagg gtgaggagcg aggtgtcctc gtcgtccgga
     4921 tgcgcggcga ccaccagcaa tcgcttgtgg gtcatgagct tggccagggc ccgctcgacc
     4981 gtggcgatgc cgccggtgcc ggccggctcc aggggctgcg gcgcgagggg gccccgggcc
     5041 tcctgggcgg agacggcgcc gacggaggcg atcatcaggg agaggacgga cgctgctatg
     5101 ggcttcacga atcgattcat cgctggctcc ggtcgttccg gtcgggaaac gaccgggagt
     5161 ctagcagggc tggccggccc agtccttgaa ccggagggtg gggtcccgtt ccaggtggcg
     5221 caggaagtcc atcaccttct ccagcaggcc gccggtctcg aggccggcgc ggcaggagtc
     5281 ctggagcgcc agcaccgccg cgatggcctc gcggctgatc tcgatctcca ggaacatctc
     5341 cgcggcctcg aggatggtgg agcaggcctc ctccggccgc ccctggcgcc ggtggatcac
     5401 ggcgaggtcg agggccgcga gcgccgagtg atagcgcttc cccacgtcca gcatcccctc
     5461 tcggacctcg acgaaggcct cctccgcctc gtccagcagt cccaggccgg cgtcggcgcg
     5521 cgcctcgatc cagcgcagct tgaggaagtc gatgcggtgc tcgcgctcgg cgaaccgccg
     5581 caggttccgg tgcgccaggg cgcgcgcctc ctcgaacctg cccgtctcga tcatgaagag
     5641 ggagaggttg tgggtcgccc agagggccag gttggggtcg cggccggagt cgatccggga
     5701 cagcccctcc tcgatgagcc ggatggcgcc ctcgggatcg ccggagtagc cggtgaacag
     5761 ccccttgctg agcagggccc ggcccgccag gtgctcgtcc cccctctcct gaaagatcgc
     5821 gaagacccgg tcgaggagcg cgatcgcctc cgggaaccgc cgctggtcgg cccggaagga
     5881 ggcctggaag tcgagcagcc gggcctgcag gctgcggtcg cggctgccgg ccgcgaagtg
     5941 gttgaaggct tcggtgaagg cgaactccgc cgccgacagg ttgtccgcca cccggtaggc
     6001 gttgcccagg atcgcccagg cgcggcagcg caggtccgcg agcccccgcg cgccgagctc
     6061 ttccggatcg agccggctgc cgaaccgggt cgcccaccgg gccgactcga tcgcctgcgc
     6121 cgggtcggcg tggaggaccg cccagctatg ccgcaggaag gcctcgaagg cgacggggcc
     6181 gcgcaggcgc tccggccccc gggcgagtgc ctcgggccca cgctccgcca gcagcgccaa
     6241 cgcttcctcc aggaggccgg ggtctaccgt ctgctgtcgt tcggcgagtg tcaggaaggg
     6301 atcacccacg aagctcctcc cggagtgggg cgccgcggat tattccatat ccgcctttcc
     6361 tgctacgctg aatggactat gtccgaggtc cagcccgacg tccagtgcga cgccgagatc
     6421 cagcggatcc tcactgtcct gcggacgtcg atgcggatcc tcggcgtcac caaccgggag
     6481 atcgagaagc ggctcggggt gagcgtcggc tatctgagcc ggctcttcca cggcagtgtg
     6541 gagctcaagg tggagcacgt cctggcgatc acgcaggcga tcgggctgca cccggccgag
     6601 cttttccatc tgctgtaccc gcagctgccg gcccggccca ccgaggcttc gctgaagctg
     6661 cggtccgccc tcgaagggtt ccgtcccgcg ccgccccctt ccccggcggc gccggagaag
     6721 cccggagccc acgcgccgtc cgaggaggac ctcgaacggc tgatgatgtc ctcccttcgc
     6781 aagctcctcg ccggcctcgg ccgcgagccg ggctgagtcc ggcctgctca agccccctgc
     6841 agggcgttct tcggatcgac ccccgcggcg cgccgcgccg gcacgacgga ggcgaggagc
     6901 gccgcccccg cgaggaccgc gacaaccgag gcgagggtga ccgggtccat gcgcccgacg
     6961 cctaccaggt tcgccgacag cacccgcgtc gccacggcgg a
//
LOCUS       DASPJI010000204         5273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_11956, whole genome shotgun sequence.
ACCESSION   DASPJI010000204 DASPJI010000000
VERSION     DASPJI010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11956"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VEL74_05345"
     CDS             complement(<1..888)
                     /locus_tag="VEL74_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61983.1"
                     /translation="MPEAEIQEFQAIALLVGFDAGTVEVCRRLLENDLRLLVADGEAD
                     ARPLLETHLIAVLCLGPGVPGQRALALIEEAEGDGTAPPVAAGGMGRLNIVFAADEPT
                     LFQDLIDRDRIFYLTQEPVPTADLVAILRSAAERWHAVARSWQGEERARAALGRILLA
                     SVRDVTSQKTAVAAGRAAAGAVVDVAEADRGYCLFYDPAKEILWAGEEGSTDQRHESA
                     AVGLVSFVTRTGRPVVLERLAGDPRFDRDADDPESTGSERFAAVPVVLPRELEPAGGR
                     GGVLAVLVAVRDVARPPFSE"
     gene            complement(1066..3687)
                     /locus_tag="VEL74_05350"
     CDS             complement(1066..3687)
                     /locus_tag="VEL74_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYG61984.1"
                     /translation="MNQALSDPLAGSALHHYQLLEKLGSGGMGQVWLALDRRLGRRVA
                     IKLLPPDVVADGPRRKRFERESRALAALNHANIVTIHSIEEADGQPFLVMEWIEGKSL
                     EDLIPLDGLPLERCLELAIPMVDAMAQAHRAGIVHRDLKPANVMVRDDGVVKVVDFGI
                     SKMEEVTVAEGFLPASTEFKTAEGIAVGTLPYMSPQQLEGRAVDARSDIFSLGVVLYE
                     MATGRRPFEGESSASLITAILRDPPPPFSRLRGTVASARLEAIVLRCLAKDPARRYQS
                     AEELAGALGELRRDPTSPALSALDMTLPDLPSPVLSAPTLTGWSLPANASATASSAAR
                     ATGASAAGPSVAHTVAPPYGSAGGRSGRLWRWAVLPALGAGLALALVTVARREAPVEL
                     SPDAGFRGGPELALPVSTTALPAVAVAVLPLANLSGDPGQEYFSDGTTEAVITNLAKI
                     SGLRVTSRTSVMRFKGSRSPLPQIARELGVSYVLEGSLAKMADQVVITAQLVDAATDS
                     VVWSDRFQGGVRDIFTFQDKVAEEVAQAARVQITGDDRSRLQQGQAVRPETYELYLKA
                     RFLINQRTPEALREALGRLDEVLKQEPGYALAWAAKAECYAHLVSPGLASMPAREGGA
                     QARKAAARAIELDPNLSDAHVVLGFATTESWEWAAAERSFRRAIELNPSNADAYLKYA
                     FYLTAMGRHAEAIKALETAKRLDPLSPAVNQALAAGHRFAGDHDRAVAAARATLDIHP
                     GFWLGHFLLGTSYSSQGRFAEADAELRRALEKGPGPVILGAIGRNQARWGKAAEARKV
                     LAELEEKKRTGYVAPTVLAKLHFALGETDQGFTWMQKALEERDQGLMFLRVDSDYAAV
                     RSDPRFARLLKEVGLNG"
     gene            3823..4461
                     /locus_tag="VEL74_05355"
     CDS             3823..4461
                     /locus_tag="VEL74_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61985.1"
                     /translation="MSDHQEAPSKPRREDRVQQAFDRAAEGANGAAGLGLKLEPAQAG
                     EGGDDVRIVGTAFVAFSKAIDYDLMDSPPPVAAADDAAITSIVTAYESILPVDLAANW
                     RQYATDPHIAIADGWVAQVTDKTFRKWLRSDEYEEWTEEYPMFSPRHVQITNLNIIYI
                     GRVRATVTYVLQEEFTNGKVAAGNGSASLFKLEGGQWRIVSIHKRVKEEVAQ"
     gene            4410..>5273
                     /locus_tag="VEL74_05360"
     CDS             4410..>5273
                     /locus_tag="VEL74_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HYG61986.1"
                     /translation="MANRLDPQAGQGGGRAVAAPTAGTAKRFLEGTHRIRPPRETWES
                     LQPVLPLAGITRVADVTGLDCIGLPVYQAVRPASLNLSVSQGKGASPDAARVSAAMEA
                     LELWHAEDLGHLPQVTLSFREARHVNPIPLGSLRWSPTLVCPGGLRVAWVHARSLRNG
                     EGDRQGWLPRAMLELDFRLGPEAAPSPFRQTSNGLASGNCREEALLHAVCELVERHAM
                     ALAQREPRRRVPLIEESVDAPWCRELIGRIREAGMRLAMRDITCEIGLPTFVADLAAP
                     DLPRVWRGSGCH"
BASE COUNT          767 a         1893 c         1888 g          725 t
ORIGIN      
        1 ctcggagaac ggcggccgcg ccacgtcgcg cacggcgacg agcaccgcca gcacgccgcc
       61 ccgccctccc gccggctcga gctcgcgcgg caggaccacg ggcacggcgg cgaagcgctc
      121 gctgccggtg gactccgggt cgtcggcgtc gcggtcgaag cgggggtcgc cggcgagccg
      181 ctccaggacg accggccggc cggtgcgggt gacgaagctc accagcccga cggcggcgct
      241 ctcgtggcgc tggtcggtcg agccctcctc gccggcccag aggatctcct tcgccgggtc
      301 gtagaacagg caatagcccc ggtcggcctc ggccacgtcc acgacggccc ctgccgccgc
      361 ccggcccgcc gccaccgcgg tcttctggga ggtgacgtcc cgcaccgagg cgaggaggat
      421 gcggccgaga gccgcccggg cccgctcctc cccctgccag ctccgggcga cggcgtgcca
      481 acgctcggcc gcgctgcgca gaatcgccac caggtcggcg gtcggcaccg gctcctgggt
      541 gagatagaag atccggtcgc ggtcgatgag gtcctgaaag agggtgggct cgtccgcggc
      601 aaagacgatg ttcaggcggc ccatgccgcc ggcggccacg ggaggggcgg tgccatcccc
      661 ctcggcctct tcgatcaggg cgagggcgcg ctggccggga acgccgggcc cgagacagag
      721 caccgcgatc aggtgggtct cgaggagcgg acgggcatcc gcctccccgt cggcgaccag
      781 cagccgcagg tcattctcca ggagccgccg gcagacctcg accgtaccgg cgtcgaagcc
      841 cacgagcaac gcgatcgctt gaaattcctg aatctccgcc tccggcatcc tgctgcgtct
      901 gctgctgatt ctgctgctga tcgtggtcaa cgggccctaa atggcagtct agaagcagct
      961 tactggatgg tcaaccgggg agaggaggag aaatcttcca ggcccctccc cgcctccctc
     1021 catcttctag gtgcgtggaa aggggccgga gggggtcacg gcgcctcagc cgttcaggcc
     1081 gacctctttg agcagtcgcg cgaagcgggg atcggagcgg acggccgcgt agtcggagtc
     1141 gacgcggagg aacatcagac cctggtcgcg ctcctcgagg gccttctgca tccaggtgaa
     1201 gccctggtcg gtctcgccga gggcgaagtg gagcttggcg agcacggtgg gggccacgta
     1261 gcccgtccgc ttcttctctt cgagctcggc cagcaccttg cgcgcctcgg cggccttgcc
     1321 ccagcgcgcc tggttgcggc cgatcgcccc caggatcacc gggccgggcc ccttctccag
     1381 cgcccgccgg agctcggcgt cggcctcggc gaagcgcccc tggctggagt acgaggtgcc
     1441 cagcaggaag tggccgagcc agaagccggg atggatgtcg agggtggcgc gggcggccgc
     1501 gaccgcccgg tcgtggtcgc cggcgaaacg gtggcccgcg gccagcgcct ggttgaccgc
     1561 gggcgacagc ggatcgagcc gcttcgccgt ctccagcgcc ttgatcgcct ccgcgtgccg
     1621 gcccatcgcc gtcagataga aggcgtactt gagataagcg tcggcgttgc tgggattcag
     1681 ctcgatggcg cgccggaacg acctctccgc cgccgcccac tcccagctct cggtggtggc
     1741 gaagccgagg accacgtgcg cgtcggacag gttggggtcg agctcgatag ccctggccgc
     1801 cgccttccgc gcctgggctc ccccctctcg cgccggcatg ctggcgaggc ccggcgagac
     1861 caggtgggcg tagcactcgg ccttggccgc ccaggccagg gcgtagcccg gctcctgctt
     1921 cagcacctcg tcgaggcggc cgagcgcctc gcgcaacgct tcgggggtgc gctggttgat
     1981 caggaagcgg gctttcagat agagctcgta ggtctccggc cgcaccgcct gcccctgctg
     2041 caggcggctc cggtcgtcgc cggtgatctg gacgcgggcg gcctgggcca cctcctcggc
     2101 caccttgtcc tggaaggtga agatatcccg caccccgccc tggaagcggt cgctccagac
     2161 cacgctgtcg gtggccgcgt ccacgagctg cgcggtgatc accacctggt ccgccatctt
     2221 cgcgagtgat ccctccagga cgtagctcac ccccagctcg cgggcgatct gcggcagcgg
     2281 cgaccggctc cccttgaacc gcatgacgga ggtgcgggag gtcacgcgca ggccgctgat
     2341 cttggccagg ttggtgatga ccgcctcggt ggtgccgtcg gagaagtact cctggccggg
     2401 gtcgccggac aggttggcca gcggcaggac cgccacggcc acggccggca gtgcggtggt
     2461 ggagaccggc agggccagct ccggaccgcc gcggaacccg gcgtcgggcg acagctccac
     2521 cggcgcctcg cggcgcgcca cggtgaccag cgccagggcc agcccggcgc cgagcgccgg
     2581 gagcacggcc cagcgccaga gccggcccga ccgccctccc gcggatccat aagggggtgc
     2641 gacggtgtgg gcgacggagg gcccggcggc ggaagcaccc gtggcccgcg ctgcagacga
     2701 cgccgtcgct gaagcgttgg ctggcagcga ccagccggtc agcgtcgggg ccgacagcac
     2761 cggggacggc aggtcgggaa gggtcatgtc cagagcggac agcgccggcg aggtggggtc
     2821 ccgccgcagc tcgcccaggg cgcccgcgag ctcctcggcc gactggtagc gccgggccgg
     2881 gtccttggcg aggcagcgca ggacgatcgc ctccagccgc gccgacgcca cggtgccgcg
     2941 tagccgcgag aacggcggcg gcgggtcgcg caggatggcc gtgatcaggg aggcggagct
     3001 ctccccctcg aaggggcggc ggccggtggc catctcatag aggacgacgc cgagggagaa
     3061 gatgtccgag cgcgcgtcca ccgcccggcc ctcgagctgc tggggagaca tgtaaggcag
     3121 ggtgcccacg gcgataccct ccgcggtctt gaactcggtg gaggccggga ggaagccctc
     3181 ggcgaccgtc acctcctcca tcttggaaat tccgaagtcg acgaccttga ccaccccgtc
     3241 gtcgcgcacc atgacattgg cgggcttcag gtcccggtgg acgatccccg cgcggtgggc
     3301 ctgggccatc gcgtcgacca tcggaatggc cagctccagg cagcgctcca gcggcagccc
     3361 gtccaggggg atcaggtcct ccagcgactt gccctcgatc cactccatca ccaggaaggg
     3421 ctggccgtcc gcctcctcga tggagtggat ggtgacgatg ttcgcgtggt tgagcgccgc
     3481 cagcgcccgc gactcgcgct cgaaccgctt gcggcgcggc ccgtccgcca ccacgtcggg
     3541 cggcagcagc ttgatggcca cccggcgtcc cagccggcgg tccagggcga gccagacctg
     3601 ccccatgccg ccgctgccca gcttctcgag gagctggtag tggtggagcg cgctcccggc
     3661 gagcgggtcg gaaagggcct ggttcattgc aagctcaatg gagccactat cttaaccctt
     3721 cctcgcccgc gattcgacat tccggacgcc catgggtaca atcccgcgca tccaagcgtc
     3781 cgagagccgg cagcttttca ttgtttccag gagggatgtt ccatgtccga ccaccaggaa
     3841 gctccatcca aaccccgccg cgaagaccgc gtccagcagg cgttcgaccg tgccgcagag
     3901 ggcgcgaacg gcgcggccgg cctgggcctg aagctggagc cggcccaggc cggagaaggc
     3961 ggcgatgacg tgcgcatcgt cggcaccgcg ttcgtcgcct tcagcaaggc gatcgactac
     4021 gacctgatgg actcgccgcc gcccgtggcg gccgcggacg acgccgccat caccagcatc
     4081 gtcacggcct acgagtccat cttgccggtc gacctggccg cgaactggcg gcaatacgcc
     4141 acggatcccc acatcgccat cgccgacggc tgggtcgccc aggtcaccga caagacgttc
     4201 cggaagtggc tgcgcagcga cgagtacgag gagtggaccg aagagtaccc gatgttctcg
     4261 ccgcggcacg tccagatcac gaacctgaac atcatctaca tcggcagggt ccgggcgacg
     4321 gtgacgtacg tcctgcagga ggagttcacc aacggcaagg tggcggccgg caacggctcg
     4381 gcctcgctgt tcaagctgga ggggggccag tggcgaatcg tctcgatcca caagcgggtc
     4441 aaggaggagg tcgcgcagta gcggccccca ccgccgggac ggccaagcgg ttcctcgaag
     4501 ggacgcaccg catccgcccg ccgcgggaga cctgggagtc cctgcagccg gtgctgccgc
     4561 tcgccggcat cacccgcgtc gccgacgtga ccggcctcga ctgcatcggc ctgccggtct
     4621 accaggcggt ccgtccggcg agcctcaacc tctcggtctc ccaggggaag ggggcgagcc
     4681 cggacgccgc ccgggtcagc gccgccatgg aggcgctcga gctctggcac gccgaggatc
     4741 tggggcacct cccgcaggtg accctgtcgt tccgcgaggc gcgccatgtg aacccgatcc
     4801 cgctcgggtc actgcgctgg tcgccgaccc tggtctgccc cggcggcctg cgggtggcct
     4861 gggtgcacgc ccgctcgcta cggaacggag agggggaccg gcaggggtgg ctcccccgtg
     4921 ccatgctgga gctcgacttc aggctggggc cggaggcggc gccgagcccg ttccggcaga
     4981 cctcgaacgg cctcgcctcg ggcaactgcc gggaagaggc gctcctccac gccgtctgtg
     5041 agctggtgga gcgccacgcg atggcgctgg cgcaacgcga gccgcggcgg cgggtgccgt
     5101 tgatcgagga gtcggtggac gccccctggt gccgcgagct catcggccgc atccgtgagg
     5161 ccgggatgcg gctggcgatg cgcgacatca cctgcgagat cggcctcccc accttcgtgg
     5221 ccgacctcgc cgcccccgac ctcccccggg tctggcgcgg ctcgggctgc cac
//
LOCUS       DASPJI010000205        13854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_413751, whole genome shotgun sequence.
ACCESSION   DASPJI010000205 DASPJI010000000
VERSION     DASPJI010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13854)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13854
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413751"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VEL74_05365"
     CDS             complement(<1..816)
                     /locus_tag="VEL74_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002517423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HYG61987.1"
                     /translation="MATSLRSSTPLLLSLLAALPGLTGCASTAPAREPERPAARQAAR
                     QEAAAGESEPYPSTYRPLPSAPVLITNATVLTAAGDRIEGGSVLMRDGKIAAVGRGIA
                     PAAGVTVVDASGKWVTPGIIDAHSHLGVYPAPGSAQAHQDGNEITDPVTAEVWAEHSV
                     WPQDPQFPLAIAGGVTTQQILPGSANLIGGRSVVLKSVPARSVQAMKFPGAPHGLKMA
                     CGENPKRVYGGKGRAPSTRMGNMAGYREAFIEAREYARDWDEYEADKEGDADPP"
     gene            951..1337
                     /locus_tag="VEL74_05370"
     CDS             951..1337
                     /locus_tag="VEL74_05370"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HYG61988.1"
                     /translation="MRNLLPGALLALGLFAGCAASGPPVTTEQLTEAEAALRSAESAG
                     AEEHAPELLQESRSALEAARRASSRGDAEGARRLLLESQDYAAAAAARARADRTRSEA
                     ERLRREADELEARTRDIMEEARRPPV"
     gene            1346..2518
                     /locus_tag="VEL74_05375"
     CDS             1346..2518
                     /locus_tag="VEL74_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYG61989.1"
                     /translation="MRNRKLGSSFNSLAGVLALAAVIAVIASLGACATAQPPAAIADA
                     RTVLEAARADRAEELATRPFDEAARHLNVAESTWRQNRDGEGAAHWARRAEAAARRAQ
                     YEALARAAEEEIRLQTERRNRAELAVRDAEIARLQAQGRNEAEQRAAEAEREARRAER
                     RAEEERAARERATEEARREAEARAEAEAQAREERLRREEELERRETQAEQERLAAEAR
                     LEEERRRLDEQRRAEEARTAEIERLRQEQEQTREELRTTLSRLAEVRQEARGLVVTLP
                     GSIYFAVNKSVVQPSMRSRLTEIAGALAGADDSTILIEGHTDSDGSNEYNLELSRLRA
                     EAVRSVLVAGGVPAGSIETQGYGETRPVAPNTSASGKAQNRRVEIIIQGGAASPGR"
     gene            2599..5058
                     /locus_tag="VEL74_05380"
     CDS             2599..5058
                     /locus_tag="VEL74_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HYG61990.1"
                     /translation="MSFSLRDHDSSPLPDPLEAAPEVGNEQFTKASILRDYRIAFRSR
                     QASLLGRKEVFTGKAKFGIFGDGKEVAQVALARAFRKGDFRSGYYRDQTLMMALGLLT
                     LEQFFAQLYAHADVELEPCSAGRSMTAHFSTCMLDADGGFRDLAQLVNSSADVSPTGS
                     QMPRLVGLAYASRLYRELPELREMTRFSNNGDEVAFGTIGNASCAEGMFWEAVNAVGV
                     LQSPMLLSIWDDGYGISVPNEFQITKSDLSAVLSGFQRAPGTRQGYDLYAVKGWDYPA
                     LCETYLNAVQIVRREHVPAIVHVTEVTQPQGHSTSGSHERYKTKERLAWEAEFDGLRK
                     MRQWILEQGIAEAAELEAAEKEDTRLVREAQDRAWQAFRNPHEEERRTVVALLGDLGR
                     ESSRGQEVEKVRQELERASLPLRRDLMAAIQSALIATCGEELAAARRLIDWKREHDRM
                     SEDRYDSDLYSESPLSALKVREVPAVYADDAPVLNGFEVVNALFDTALRRYPHLIAMG
                     EDVGKIGDVNQGFAGLQAKYGPLRVSDTGIRETTIIGQAIGMALRGLRPIAEIQYLDY
                     LLYALQILSDDLATLRYRTKGRQMAPVIVRTRGHRLEGIWHAGSEMGGIVHLIRGIYF
                     CVPRNMTQAAGFYNTLLQSDDSGIIVEVLNGYRLKERLPANLEDFTVPLGVPEVLREG
                     SDVTVVTYGACCRIAMEAAERLAEVGIETEVIDVQTLLPFDLPGRIVESLKKTNKVLF
                     LDEDVPGGGSAYMMQRVLEEQGGYDWLDAAPRTLSGKEHRPAYGSDGDYYSKPNRETV
                     FEAVYEIMHETDPASFPIFYR"
     gene            complement(5103..7145)
                     /locus_tag="VEL74_05385"
     CDS             complement(5103..7145)
                     /locus_tag="VEL74_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HYG61991.1"
                     /translation="MSRKMLINAQRPEELRIAIVEDGVLEDYQVDVAEAGVTRGNIYR
                     GVIANIQPSLNAAFIDYGIDRHGFLAIQDVVPDAYYHQPKGHTPRIEEVLERGKPIVV
                     QVAKDAIGSKGASLTTSLSLAGRYLVLTPFEDTRGVSRKVEDDDTRKKLKVLAAGPEL
                     PDGCGIIVRTNALGQTKATLQRDLAALLRLWKRVQSAAVDGRGVRLLYTDQDLIFQAL
                     RDRLDASIEELLVDDDEAFQKAQAYMQAFMPRAKTKLIRYTDRLPLFSRFQLEPQIDR
                     IYDRTVPLPSGGSLVIDATEALTAIDINSGRSTRAATQEETALHTNLEAAAEVARHLR
                     LRDIGGLVVVDFIDMRASKYQRRVEKALRDAMKDDKARSTVGRISPNGLLEINRQRIQ
                     QALQVRTHRPCPTCNGMGRLASPEMVTLNLLRRVEERAAAGTLQGVRIGLHPELADAM
                     QNSRRRELAALEEEFDLKIEIIAAAGLHRPDQQIEWFQREKPVTPRRTKPAPSILRPW
                     DMALPGPAEDDFDEEDEEPAPAAAKPAKPAGRQPRSEPRQQRQPRQARPEPHPLPADS
                     GQAETEEEKRSRKRRRGGRKRKKGGAGVNVAAGDGNGDGEPAFTDGAAPEGDGMDHDF
                     VEGDSEEGGLEAEGGGGRSSNKRRRRRKRRGGARHQGDDAPGAAPEPSEPPPPTVH"
     gene            complement(7689..8036)
                     /locus_tag="VEL74_05390"
     CDS             complement(7689..8036)
                     /locus_tag="VEL74_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG61992.1"
                     /translation="MHDHDHGTPSGDRTPTSIESLAPTEATRRALYDAVVEVHFQPSA
                     EEIQRAQEDPENHWIPRYTPDEAGLAILWIFGRWFVVWRALEVTEGPETLKWTVLRVA
                     PEGIPLPVQFLEV"
     gene            8307..9533
                     /locus_tag="VEL74_05395"
     CDS             8307..9533
                     /locus_tag="VEL74_05395"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG61993.1"
                     /translation="MTGYRHREIAGLVEEALRSLPIVVLTGLRQAGKTTFLEKDAAVA
                     GRRYFTLDDFATLETARREPEALISGDEPVTVDEVQRSPDLLLAVKREVDRRRAPGRF
                     LLSGSANLALMEGISDSLAGRALYLTLLPLSRRERTGALEERPFLVRFLEDPKLPPRT
                     EAAPVSGDEILDGGLPPVALGDAVSRELWFLGYEQTYLERDLRDLSQVADLVSFRNVL
                     RLAALRTGQILNQSELARDARLPASTVSRYLGLLEASFVLARIAPHLRSRATRLIKSP
                     KVFMTDSGLAAHLGAVTDLEPIADEPLRGALFETYTLQNVAAILSAHLPRARVDFWSV
                     QGRHEVDFVLTSGRTSVGIEVKAATRFSERDLSGLRVFLEKTPGARAGVLAYNGTDSV
                     ALGENLFAIPLGLLLS"
     gene            9631..11166
                     /locus_tag="VEL74_05400"
     CDS             9631..11166
                     /locus_tag="VEL74_05400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYG61994.1"
                     /translation="MKHYDVVILGGAFSGASAAILLRRDCPQLSVLVVERAEAFDAKV
                     GEATTEMSGMFLTRRLAQWQHLEREHLPKEGLRYWFQNEKVHCHAEASEFGAFQRSTV
                     PSFQLRRDVLDEHLLATAAAEGAELVRPARVREVEMGSFDHRVTFEKDGQPETVSCRW
                     VLDATGRATLLGKKLGLIERNKEHPTASIWCRWEDVRHLDDVAARGPLAFSRGNVSSR
                     RLATNHYMGYGYWVWFIPLGNGETSIGIVFDTRLVDIDRGSGMAEAYLSFLHAIPMAA
                     ELLEGARMRAEDLRTFSNLAYATRQYMGDGWALLGDAAVFLDPYYSPGLDHASFSVEA
                     TVEIVKAQSEGQDVAARIAEHNQTFLRSYWRFFRSIYKDKYFYMGEHDLLSASVLIET
                     AQYYIFVVKPAYLRHGRFQWMPVLGPKPAFTSYHMMRIYNRRFKAITLARRAAGEAGR
                     RNHGRRISALFDLRLSPFRMAARGLKIWAFAEIDNARLAIRRLFGRTPAAGERVEERP
                     ADA"
     gene            11239..>13854
                     /locus_tag="VEL74_05405"
     CDS             11239..>13854
                     /locus_tag="VEL74_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S9"
                     /protein_id="HYG61995.1"
                     /translation="MPMQVPCLRRAVPRLALALALFTLLPVTASAQYFGRNKVQWERF
                     DWQILRTEHFDIYYYPQEEQAVREAARLAERWYDRLSAIFERPFNERKSIILYADPSD
                     FQQTTVIGGQIGQGTGGVTEALRTRVVLPFTGNWEDTDHVLGHELVHVFQYDILQDPR
                     LQGPGRGGGGGAGIPLWFVEGLAEYLSIGRSSSLTAMWLRDSVARDELPTVRQLARNP
                     RLFPYRWGHALWAYIGGRWGDVATTRVFTRGTQVGLEAALEEVLGISHEDLSTDWQAS
                     IRRDYGPIVEGRQAPANLGTRIIPREGPTTETETNVGPVLSPNGQDVVFFSTRGLFSF
                     DLYLADARTGEIRQRLVSQDTDPHFEALQFIDSAGAWSPDGRRFAFVVSARGDNELAI
                     VEVATRNIERRIPIQDVGALWNPSWSPDGRSIVVSGARGGITDLYLHDLESGTTRKLT
                     DDLFSDLQPDWSPDGRTIAFVSDRGAGESLAGLTRGTAMGVWLLDVASGETRQIAAEF
                     EGNQYNPRFGPGGRDLFFLSDRDGVSDVYRLETGSGQIFRVTRVATGVTGITGLSPAL
                     TVAPSSGRVMFSVFTNTGYQINSLEPAETRGEPVDTGGATRAQAALLPPAARPAPPRV
                     DQYLASPAGLEPGPTETAEYRPRLQLDFVGPSVGVGVSTLGYSFGGDITAFFSDMLGQ
                     REVGVSVQGASGSDINQLGGQAYYLNQERRWQWGVSGGYVPYISAFTRAFETDVDVGG
                     GEVAPGIVYQQVRETVTVSGASFITRYPFSTTRRFDFNVGVTRLDYDNELRELVTVGS
                     QVIDENERDLASPEGLQLYQASAALVGDNSYFGFTSPVRGRRYHLELESSFGDLQFQS
                     LIADYRRYFFARPVTF"
BASE COUNT         1975 a         4915 c         4829 g         2135 t
ORIGIN      
        1 gggcggatcg gcgtcaccct ccttgtcagc ctcgtactcg tcccagtcgc gggcgtactc
       61 ccgggcctcg atgaaggcct cgcggtagcc cgccatgttg cccatgcggg tggacggggc
      121 gcgccccttc ccgccgtaga cccgcttggg gttctcgccg caggccatct tcagcccgtg
      181 gggggcgccc gggaacttca tcgcctggac gctgcgggcc gggacgctct tgaggaccac
      241 cgagcggccg ccgatcaggt tggccgagcc gggcaggatc tgctgcgtgg tgacgccgcc
      301 cgcgatggcc agggggaact gcgggtcctg cggccagacg gagtgctcgg cccagacctc
      361 ggcggtcacc gggtcggtga tctcgttgcc gtcctggtgc gcctgcgccg accccggcgc
      421 ggggtagacg ccgagatggg agtgggcgtc gatgatcccc ggcgtcaccc acttgccgga
      481 ggcgtccacc accgtgaccc ccgcggcagg tgcgatcccg cggccgacgg cggcgatctt
      541 gccgtcccgc atcaggaccg agccgccctc gatccggtca ccggcggcgg tgaggacggt
      601 ggcgttggtg atcaggaccg gcgccgacgg cagcggccgg taggtggagg ggtagggctc
      661 gctctcaccg gcggcggctt cctgcctggc ggcttgcctg gccgccgggc gctccggctc
      721 ccgcgcgggg gcggtgctgg cgcagcctgt caggcccggg agggcggcga gcagggagag
      781 caggagagga gtggagctgc ggaggctcgt tgccatgctt ttttcctcgt gtccgtgcgg
      841 tgtccgtgcg cgcggcatcc tagcacggcc gcccccggcc gcctcgctgg cacccctcgt
      901 gcaccaacgt cgggagtggg agcgcggtgc ggacgaggaa ggagcctgcc atgagaaatt
      961 tgttgcctgg ggcgctgctc gcgctgggtc tgtttgcggg atgtgccgcc agcggccccc
     1021 ccgtcactac ggaacaattg accgaagccg aggccgcgct gcggagcgcc gagtccgcgg
     1081 gcgccgagga gcacgccccc gagctgctcc aggagtcgcg ctcggccctc gaggccgccc
     1141 ggcgcgcgtc gtcccgcggc gatgcggagg gggcgcggcg gctgctgctc gagtcccagg
     1201 actacgcggc cgccgccgcg gcccgggccc gtgccgacag gacccggagc gaggccgagc
     1261 gcctgcgccg ggaggccgac gagttggagg ccaggacccg agacatcatg gaagaggcgc
     1321 gccggccgcc ggtatgaggt gctgtatgag aaataggaaa ctcggatcgt cgttcaactc
     1381 gctggccggg gtgttggccc tggccgccgt catcgccgtc atcgcatcgt tgggcgcctg
     1441 cgccacggcg cagccgccgg ccgccatcgc cgacgcgcgg accgtcctgg aagccgcccg
     1501 tgccgaccgc gccgaggagc tcgcgacccg tcccttcgac gaggccgccc ggcacctgaa
     1561 cgtggccgag agcacctggc ggcagaaccg ggatggcgag ggcgcggcgc actgggcgcg
     1621 gcgggccgag gccgcggcgc gccgggcgca gtacgaggcg ctggctcgtg ccgccgagga
     1681 ggagatccgt ctccagaccg agcgccgcaa ccgtgccgag ctggcggtgc gcgacgccga
     1741 gatcgcccgc ctgcaggccc aggggcgcaa cgaggcggag cagcgcgccg ccgaggccga
     1801 gcgggaggcg cggcgggccg agcgccgggc cgaagaggag cgggctgcac gcgagcgcgc
     1861 caccgaggag gcgcggcgtg aggccgaggc gcgcgcggag gccgaggccc aggcgcgcga
     1921 ggagcggctg cggcgggagg aggagctcga gcgccgggag acccaggccg agcaggagcg
     1981 gctggccgcc gaggcgcgtc tggaggagga gcgccgccgg ctcgacgagc agcggcgggc
     2041 tgaggaggcc cggaccgccg agatcgagcg cctgcgccag gagcaggagc agacccgcga
     2101 ggagctgcgc accaccctgt cccgcctcgc cgaggtgcgc caggaggcgc gcggcctggt
     2161 ggtgaccctc cccggcagca tctacttcgc ggtcaacaag tcggtggtcc agccgtccat
     2221 gcgctcgcgg ctgacggaga tcgccggcgc cctggccggc gccgacgact ccaccatcct
     2281 tatagaaggg cacaccgact ccgacgggtc gaacgagtac aacctggagc tctcccggct
     2341 gcgggccgag gcggtgcgct cggtgctggt cgccggcggc gttcctgccg ggagcatcga
     2401 gactcagggc tacggcgaga cgcggccggt cgcccccaac accagcgcct cgggcaaggc
     2461 ccagaaccgg cgggtcgaga tcatcatcca gggaggggcg gcctcgcccg gccgctgagc
     2521 cgcgcggggg gcgcgtggta gcattgcccg gttggcccgg cggagccggg cgtcccaaaa
     2581 accccggagg ctccctgcat gtcgttttct ttgagggatc acgattcgtc gcccctcccc
     2641 gacccgctcg aggccgcgcc cgaggtgggc aacgagcagt tcaccaaagc cagcatcctc
     2701 cgcgattacc ggatcgcgtt ccgcagccgg caggccagcc tgctcggccg taaagaagtg
     2761 ttcaccggca aggccaagtt cggcatcttt ggcgacggca aggaggtcgc ccaggtggcc
     2821 ctcgcccgcg ccttccgcaa gggggatttc cggtccggct actaccgcga ccagaccctg
     2881 atgatggcgc tcggcctgct caccctggag cagttcttcg cccagctcta tgcccacgcc
     2941 gacgtcgagc tcgagccctg ctcggccggc cgctcgatga cggcccattt ctcaacctgc
     3001 atgctcgacg ccgacggcgg cttcagggac ctggcgcagc tcgtcaactc ctcggcggac
     3061 gtctcgccca ccggctcgca gatgccgcgc ctggtcggcc tcgcctatgc ctcgcggctc
     3121 taccgcgagc tgcccgagct gcgggagatg acccgcttct ccaacaacgg cgacgaggta
     3181 gccttcggca ccattggcaa cgcgagctgc gccgagggga tgttctggga ggcggtcaac
     3241 gccgtcggcg tcctccagtc ccccatgctc ctctccatct gggacgacgg ctacggcatc
     3301 tcggtcccca acgagttcca gatcaccaag tcggacctct ccgcggtgct ctccggcttc
     3361 cagcgcgccc ccggcacccg ccaggggtac gacctctacg cggtcaaggg gtgggactac
     3421 ccggcgcttt gcgagaccta cctcaacgcg gtgcagatcg tgcgccgcga gcacgtgccg
     3481 gcgatcgtcc acgtcaccga ggtgacccag ccccaggggc actccacctc cggtagccac
     3541 gagcgctaca agaccaagga gcgcctggcc tgggaggccg agttcgacgg cctgcgcaag
     3601 atgcggcagt ggatcctgga gcagggcatc gccgaggccg cggagctgga ggcggccgag
     3661 aaggaggata cccggctggt ccgcgaggcc caggaccggg cctggcaggc cttccgcaac
     3721 ccccatgagg aggagcggcg gaccgtcgtg gccctgctcg gcgacctcgg ccgggagtcc
     3781 tcgcgcggcc aggaggtgga gaaggtgcgg caggagctgg agcgcgccag cctgccgttg
     3841 cgccgcgacc tcatggccgc catccagagc gccctgatcg ccacctgcgg cgaggagctg
     3901 gcggcggcgc ggcggctgat cgactggaag cgcgagcacg accggatgag cgaggaccgc
     3961 tacgactcgg acctctacag cgagtcgccg ctgtccgccc tcaaggtgcg ggaggtgccg
     4021 gcggtctacg cggacgacgc gccggtgctc aacggcttcg aggtggtcaa cgccttgttc
     4081 gacaccgcgt tgcggcgcta cccccatctg atcgccatgg gcgaggacgt gggcaagatc
     4141 ggcgacgtca accagggctt cgccggcctc caggccaagt acggaccgct ccgcgtctcc
     4201 gacaccggca tccgcgagac caccatcatc ggccaggcca tcggcatggc cctgcgcggg
     4261 ctgcggccga tcgccgagat ccagtatctc gactacctcc tctacgcgct gcagatcctg
     4321 tcggacgacc tcgccaccct gcgctaccgc accaaggggc ggcagatggc gccggtcatc
     4381 gtccgcaccc gcggccaccg gctggagggg atctggcacg ccggctcgga gatggggggc
     4441 atcgtccacc tgatccgcgg catctacttc tgcgtgccgc gcaacatgac ccaggcggcc
     4501 ggcttctaca acaccctgct ccagtcggac gactcgggga tcatcgtcga ggtgctgaac
     4561 ggctaccggc tgaaggagcg cctgcccgcc aatctcgagg acttcaccgt gccgctgggc
     4621 gtgcccgagg tgctgcgcga ggggagcgac gtcacggtcg tgacctacgg cgcctgctgc
     4681 cgtatcgcca tggaggcggc cgagcggctg gccgaggtgg ggatcgagac cgaggtgatc
     4741 gacgtccaga cgctgctgcc gttcgacctc ccggggcgga tcgtggagtc gctcaagaag
     4801 accaacaagg tgctcttcct ggacgaggac gtgccgggcg gcggcagcgc ctacatgatg
     4861 cagcgggtgc tggaggagca ggggggctac gactggctgg acgcggcgcc ccgcaccctc
     4921 tcgggcaagg agcaccggcc cgcctacggc tcggacggcg actactactc gaagccgaac
     4981 cgcgagacgg tcttcgaggc cgtctacgag atcatgcacg agaccgatcc ggcctcgttc
     5041 cccatcttct accgctagaa aagcgaaggg gcgccgcgcg gcgccccttc ctgtcttcgg
     5101 cctcagtgga ccgtcggcgg cggcggctcc gagggctccg gtgccgcccc gggggcgtca
     5161 tcgccctggt gcctggcacc gccacggcgc ttgcggcgcc ggcgccgctt gttggaagag
     5221 cggccacccc ctccctccgc ttcgagccca ccctcttcgg agtccccctc cacgaagtcg
     5281 tggtccatgc cgtcgccttc gggcgccgcg ccgtcggtga acgccggctc gccgtcaccg
     5341 ttcccatcgc cggccgcgac gttcacgccg gcgccaccct tcttgcgctt gcggccaccg
     5401 cggcggcgct tccgggagcg cttttcctcc tcggtctcgg cctgaccgga gtcggccggg
     5461 aggggatggg gctcgggcct ggcctgccgg ggctgcctct gctgccgcgg ttcggacctc
     5521 ggctgccggc ctgccggttt ggcgggcttg gcggcggccg gcgccggctc ctcgtcctcc
     5581 tcgtcgaagt cgtcctcggc ggggccggga agcgccatgt cccagggacg gaggatggac
     5641 ggagcgggct tggtgcgccg cggcgtcacc ggcttctcac gctggaacca ctcgatctgc
     5701 tggtccggac ggtgcagccc cgcggcggcg atgatctcga tcttgaggtc gaactcctct
     5761 tcgagcgccg ccagctcgcg ccgccggctg ttctgcatgg cgtcggcgag ctcggggtgg
     5821 aggccgatgc gcaccccctg gagggtgccg gcggcggccc gctcctcgac gcggcgcagc
     5881 aggttgagcg tcaccatctc cgggctcgcc aggcgcccca tgccgttgca ggtggggcag
     5941 gggcggtgcg tgcggacctg gagcgcctgc tggatgcgct ggcggttgat ctccagcagg
     6001 ccgttggggc tgatccggcc gaccgtcgag cgcgccttgt cgtccttcat ggcgtcgcgc
     6061 agcgccttct cgacccggcg ctggtacttc gacgcccgca tgtcgatgaa gtccaccacc
     6121 accaggccgc cgatgtcgcg cagccggaga tggcgggcga cctcggccgc cgcctccagg
     6181 ttggtgtgga gggcggtctc ctcctgcgtc gcggcgcggg tggagcggcc ggagttgatg
     6241 tcgatcgcgg tgagcgcctc ggtggcgtcg atgaccagcg agccgccgct gggcagcggc
     6301 acggtgcggt cgtagatgcg gtcgatctgc ggctcgagtt ggaaccggga gaagaggggg
     6361 aggcggtcgg tgtagcggat cagcttggtc ttggcccgtg gcatgaacgc ctgcatgtag
     6421 gcctgcgcct tctggaacgc ctcgtcgtcg tcgaccagca gctcctcgat actggcgtcg
     6481 agccggtcgc ggagggcctg gaagatcagg tcctggtcgg tgtagaggag gcgcaccccc
     6541 cggccgtcga ccgccgcgga ctgcacccgc ttccagaggc ggagcagggc ggcgaggtcg
     6601 cgctgcaggg tggccttggt ctgccccagg gcgttggtgc ggacgatgat gccgcacccg
     6661 tcggggagct cgggcccggc ggccaggacc ttgagcttct tcctcgtgtc gtcgtcctcg
     6721 accttgcggg acacgccacg ggtgtcctcg aagggagtga gcacgagata gcggccggcc
     6781 agcgacaggc tggtggtgag cgaggcaccc ttcgacccga tggcgtcctt ggccacctgc
     6841 accacgatgg gcttgccgcg ctccaggacc tcttcgatcc ggggggtatg gcccttgggc
     6901 tggtggtagt aggcgtcggg gaccacgtcc tggatggcca ggaagccgtg gcggtcgatg
     6961 ccgtagtcga tgaaggcggc gttcaggctg ggctggatgt tggcgatgac gccgcggtag
     7021 atgttgccgc gggtgacgcc ggcctcggcc acgtccacct ggtagtcctc gaggacgccg
     7081 tcctcgacga tggcgatgcg caactcttcc ggacgctgcg cgttgatcag cattttgcga
     7141 ctcaaagttc gctccgtttg tttctgtgag acttgatgct tcgtggaaag attcggcagg
     7201 cgggaggtca ggccaggggc taggagcggg cggcgcgcat tggcctattg tgcactttcg
     7261 ggagccgttc tgcaagctct tgcggtatgc tgctggggag cgcggctccg cgaccggagg
     7321 ctgaaggtcc gcgagagccc cgtggaggac cacccgctcc cccgaaaggg gaggacccgc
     7381 ccccgcaaag gggacgccct cctccccatt ccgggagagg ggattccctc aaagggtgcg
     7441 ccccttccca gataggggag ggctcctccc cgatccggta cggctccctc ccgcatccgg
     7501 tacagcctct tcccctttca ggtacactcc ctccccaaaa ggggagagac ccctcccctt
     7561 tgagggatgg gcctgtaccg gctccccttt gtctccattt cccgttcact gagggctgga
     7621 tggccccgga aggccgtgaa ggaccggcgg agagcggccg aggacggctc tccgccgggg
     7681 gtgtggcgtc agacttcgag gaactgcacg ggaaggggga tcccctcggg ggcgacccgc
     7741 agcaccgtcc acttcagcgt ttcgggtccc tccgtcacct ccagcgcccg ccagacgacg
     7801 aaccagcggc cgaagatcca gaggatggcg aggcccgcct cgtccggcgt gtagcgggga
     7861 atccagtggt tctccggatc ctcctgcgcc cgctggatct cctcggcgct gggctggaag
     7921 tggacctcca ccaccgcgtc gtagagcgcg cggcgcgtgg cctcggtggg ggcgagggac
     7981 tcgatagacg tgggagtgcg gtccccggac ggggtaccat gatcgtggtc gtgcacggag
     8041 tacaacctcc ttgtgcggct aggccctggg tggagtgcta gctccatccg gggccgctcg
     8101 ccgcgggttg aatgaccgga cgaagaggta ttcctcgccc ggggacagcc tggaaagaac
     8161 ggtgctgcgg tacggcttga cgaccgagga cgactttatc tcagtgaaat cggggcgtca
     8221 agggtctctg ttagagttct caataggcga ggtaaggctg aaccacatta gccaccttgg
     8281 catattccac cgtatcatgg tgggttatga caggctaccg gcaccgcgag atcgcgggac
     8341 tcgttgagga ggccctccgt tcgcttccca tcgtcgtgct tacgggcctg aggcaggccg
     8401 gcaagaccac cttcctggag aaggacgcgg ccgtcgcggg gcggcgctac ttcactctgg
     8461 acgacttcgc gactctggag actgcccggc gcgagccgga agcgctgatc tcgggggacg
     8521 agccggtcac cgtcgacgag gtccagcgaa gcccggatct cctcctggcg gtcaagcgtg
     8581 aggtcgaccg gcgacgggcc ccgggccggt ttctgttgag cggctcggcc aatctcgccc
     8641 tcatggaggg tatcagcgac tccctcgcgg ggcgggcgct ctatctgacg ctcctccccc
     8701 tcagccgccg tgagcggacc ggcgccctgg aagagcgtcc ctttctggtg cgcttcctcg
     8761 aggatcccaa gcttcctccg cgtacagagg ccgcgccggt ctccggcgac gagatcctgg
     8821 atggcggcct gccgccagtg gcgctgggag acgccgtgag ccgggagctc tggttcctgg
     8881 gctacgagca gacctatctc gagcgcgacc tgcgggacct ctctcaggtc gccgacctcg
     8941 tctcgttccg gaacgtcctg cgtttggcag cgttacggac agggcagatc ctcaaccaga
     9001 gcgagctggc acgggacgcc cggctacctg ccagcaccgt ctcgcgctat ctcggccttc
     9061 tggaggcctc ttttgttctg gcccgcatcg ctccccatct cagaagccgg gccacgcggc
     9121 tcatcaagtc gccgaaggtc ttcatgacgg attcggggtt ggccgcccac ctcggagcgg
     9181 tgacggacct cgagcccata gcggacgagc ccttgcgggg agccctgttc gaaacgtaca
     9241 cgctgcagaa cgtcgccgcc atcttgagcg ctcacctgcc gcgtgcccgg gtggacttct
     9301 ggagcgtcca ggggcgccat gaggtggatt tcgtcctgac cagcggccgg acctccgtag
     9361 ggatcgaggt caaggccgcc acccgcttca gtgagcggga cctgtcaggc ctccgggtgt
     9421 tcctggagaa gacgccgggc gcccgtgccg gcgtcctggc ctataacggc acggactccg
     9481 tggctctggg ggaaaacctc ttcgccatcc cgctggggct gctgttgtcc tgaggagacc
     9541 gccgccctcc ccccgccctg ctacaatccg ccgtgcatga catcccctgc cggggtggcc
     9601 gggtgggtgg gcgcggaaga aggtaatctc atgaagcact acgacgtcgt catcctcgga
     9661 ggggccttct ccggcgcttc ggcggcgatc ctgctgcggc gggattgccc ccaactctcg
     9721 gtcctcgtcg tcgagcgcgc ggaggcgttc gatgccaagg tgggggaggc cacgaccgag
     9781 atgtcgggca tgttcctcac ccgccgcctc gcccagtggc agcacctcga gcgcgagcac
     9841 ctccccaagg aggggctgcg ctactggttc cagaacgaga aggtccactg ccacgccgag
     9901 gcctccgagt tcggcgcctt ccagcgcagc accgtcccct cgttccagct ccgccgcgac
     9961 gtcctcgacg agcacctgct cgccaccgcc gccgccgaag gggcggagct ggtccggccc
    10021 gcccgtgtcc gtgaggtcga gatggggtcc ttcgaccacc gggtcacctt tgaaaaagac
    10081 ggccagccgg agaccgtctc ctgccgctgg gtgctggacg ccaccggccg tgccaccttg
    10141 ctgggcaaga agctcggcct catcgagcgc aacaaggagc acccgaccgc ctccatctgg
    10201 tgccgctggg aggacgtccg ccacctcgac gacgtcgccg cccgcggacc cctggccttc
    10261 tcccgcggca acgtcagctc ccggcgcctc gccaccaacc attacatggg ctatggctat
    10321 tgggtctggt tcatccccct gggcaacggc gagaccagca tcggcatcgt cttcgatacc
    10381 cgcctggtcg acatcgaccg cgggtcgggc atggccgagg cctacctctc cttcctgcac
    10441 gccatcccca tggccgccga gctcctcgaa ggcgcccgca tgcgtgccga agacctccgc
    10501 accttttcga acctggccta cgccacccgc cagtacatgg gcgacggctg ggccctcctc
    10561 ggcgatgccg cggtgttcct cgatccctac tactccccgg ggctcgacca cgcctccttc
    10621 tcggtcgagg ccacggtcga gatcgtcaag gcgcagtcgg agggccagga tgtcgcggcc
    10681 cgcatcgccg agcacaacca gacgttcctg cgctcctact ggcgcttctt ccggtccatc
    10741 tataaggaca agtacttcta catgggggag cacgacctgc tgtcggcgtc ggtcctcatc
    10801 gagacggcgc agtactacat cttcgtcgtc aagcccgcct acctgcggca cggccgcttc
    10861 cagtggatgc cggtgctggg ccccaagccg gcgttcacca gctatcacat gatgcgaatt
    10921 tacaaccggc gcttcaaggc gatcaccctc gcccggcgcg ccgcgggcga ggccggccgg
    10981 cgcaaccacg gccggcggat cagcgccctg ttcgacctcc ggctctcccc gttccgcatg
    11041 gctgcccgcg ggttgaagat ctgggccttc gccgagatcg acaacgcccg cctcgccatc
    11101 cgccgcctct tcggccggac gccggcagcc ggcgaacgag tggaggagag gccggcggac
    11161 gcatagcctc tccggcatca gttttgaatc aatcaagact ccttgggctg ttgatcttgc
    11221 cctcgaggag tctccccgat gcccatgcag gtcccctgtc tccgccgcgc ggtgccgcgg
    11281 ctcgcgctcg ccctcgctct cttcaccctc ctgcccgtca ccgcctccgc ccagtacttc
    11341 ggccgcaaca aggtccagtg ggagcggttc gactggcaga tcctgaggac cgagcacttc
    11401 gacatctatt actaccccca ggaggagcag gcggtgcgcg aggccgcccg cttggccgag
    11461 cgctggtacg accgcctgtc ggcgatcttc gagcgcccct tcaatgagag aaagtccatc
    11521 atcctctacg ccgacccgag cgacttccag cagaccacgg tgatcggcgg ccagatcggc
    11581 caggggaccg gcggcgtcac cgaggcgctc cgcacccgcg tcgtgctgcc gttcaccggc
    11641 aactgggagg acaccgacca cgtgctcggc cacgagctgg tgcacgtctt ccagtacgac
    11701 atcctgcagg acccgcggct gcaggggccg gggagagggg ggggcggtgg cgccggcatc
    11761 cccctctggt tcgtcgaggg gctcgccgag tacctgtcga tcggccgcag ctcctcgctg
    11821 accgccatgt ggctgcgcga ctccgtggcc cgcgacgagc tgcccaccgt ccgccagctc
    11881 gcccgcaacc cgcgcctctt cccctaccgc tgggggcacg cgctgtgggc ctacatcggc
    11941 ggccgctggg gcgacgtggc caccacccgc gtcttcaccc gcggcaccca ggtcgggctc
    12001 gaggccgccc tcgaggaggt cctggggatc tcccacgagg acctgtcgac cgactggcag
    12061 gcctccatcc gccgggacta cggccccatc gtcgaggggc gccaggcgcc ggccaacctc
    12121 ggcacccgca tcattccgcg cgagggcccg accaccgaga ccgagaccaa cgtcgggccg
    12181 gtgctcagcc ccaacggcca ggacgtcgtc ttcttctcga cccgcggcct gttctcgttc
    12241 gacctctatc tggccgacgc ccgcaccggc gagatccggc agcggctcgt ctcccaggac
    12301 accgaccccc acttcgaggc gctgcagttc atcgactcgg cgggcgcctg gtcgccggac
    12361 ggccgccgct tcgccttcgt cgtctcggcc cgcggcgaca acgagctcgc catcgtcgag
    12421 gtggcgacgc ggaacatcga gcggcggatc ccgatccagg acgtcggcgc gctgtggaac
    12481 ccgagctggt cgccggacgg ccgctccatc gtcgtctccg gcgcgagggg ggggattacc
    12541 gacctctacc tgcacgacct ggagagcggc accacccgca agctgaccga cgacctgttc
    12601 tccgacctcc agcccgactg gtcgcccgac ggccggacca tcgccttcgt ttccgaccgg
    12661 ggggcggggg agagcctggc gggcctgacg cgcgggaccg ccatgggggt ctggctgctc
    12721 gacgtggcga gcggcgagac gcggcagatc gccgccgagt tcgagggcaa ccagtacaac
    12781 ccgcgcttcg gccccggcgg ccgggacctc ttcttcctct ccgaccgcga cggcgtgagc
    12841 gacgtctatc gcctggagac gggctccggc cagatcttcc gggtgacccg cgtcgccacc
    12901 ggcgtcaccg gcatcaccgg gctgtcgccc gccctcaccg tggcgccgag cagcggccgg
    12961 gtgatgttct cggtcttcac caacaccggc tatcagatca attcgctgga gccggcggag
    13021 acccgcggcg agccggtgga caccggcggc gccacccgcg cgcaggcggc cctgctgccc
    13081 ccggccgcgc ggccggcccc tccgcgggtc gaccagtatc tggcgagccc cgccggcctc
    13141 gaaccggggc cgaccgagac cgccgagtac cggccccggc tccagctcga cttcgtcggc
    13201 ccctccgtcg gcgtcggggt gagcaccctg ggctacagct tcggcggcga catcaccgcc
    13261 ttcttcagcg acatgctggg gcagcgcgag gtgggcgtct ccgtccaggg ggcgagcggc
    13321 agcgacatca accagctggg cggccaggcc tactatctca accaggagcg gcgctggcag
    13381 tggggcgtct ccggcggcta tgtgccctac atctccgcct tcacccgcgc cttcgagacc
    13441 gacgtcgacg tgggaggcgg cgaggtggcc ccggggatcg tctaccagca ggtgcgggag
    13501 accgtcaccg tcagcggcgc ctcgttcatc acccgctatc cgttctcgac cacccgccgc
    13561 ttcgacttca acgtcggcgt cacgcggctc gactacgaca acgagctgcg cgagctggtc
    13621 accgtcggca gccaggtgat cgacgagaac gagcgggacc tcgcctcacc ggaggggctg
    13681 cagctctacc aggcgtcggc ggccctggtg ggggacaaca gctacttcgg cttcacctcg
    13741 ccggtgcgcg ggcgccgcta ccacctggag ctggagtcct cgttcggcga cctgcagttc
    13801 cagtcgctga tcgccgatta ccggcgctac ttcttcgcca ggccggtgac gttc
//
LOCUS       DASPJI010000206         4096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_12056, whole genome shotgun sequence.
ACCESSION   DASPJI010000206 DASPJI010000000
VERSION     DASPJI010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12056"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VEL74_05410"
     CDS             <1..971
                     /locus_tag="VEL74_05410"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG61996.1"
                     /translation="QLLLKKSIDAAFEDPGLAVRLANLAVRASAHLGEAYDPYWVMDL
                     RARALAYLGNARRVLGELQSAEEAFRKAEAWLEASTTGNVQVQAEILGFKSSLRRDQR
                     RFGEALELVERALHLYQEAGDSGGIAKCLLKKSKILEETGRFAEAIELLRGARIPRDR
                     EPLLFAYARYNLLVCLVLSGQPEEAERLLPEVRSLLAESGKPLNLIRLRWAEGLIDLG
                     MGRSGPAEAAFRGVQEEFLERGMGYDAALVSLDLAFLYAREGETKVLQHLALELMPVF
                     TSREIHREATAALLMFQHVCEEERASAGLVRQLGVFLRRERRGRGV"
     gene            complement(1311..1658)
                     /locus_tag="VEL74_05415"
     CDS             complement(1311..1658)
                     /locus_tag="VEL74_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HYG61997.1"
                     /translation="MKTVTLDVREPAAAMDAFVKSWKTGEPQESARISFATPELLWKV
                     LTAKRWEILKALCGVGPVSIREAARRVDRDVKAVHGDITALLAAGLLSRTEGGGVEFP
                     YEAVKVEFVLKAA"
     gene            1751..2764
                     /locus_tag="VEL74_05420"
     CDS             1751..2764
                     /locus_tag="VEL74_05420"
                     /inference="COORDINATES: protein motif:HMM:NF016629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HYG61998.1"
                     /translation="MSSHLTHRIEDDLGNHDLSYRLMFSHPRMIEEMLREFIAERWVE
                     TLDFSTLERVNADFVTEGLKGREGDLIWKLRRRDGRAVYLYLMVEFQSKVDRFMAVRL
                     MGYESLLYQELITRSELSSDGRLPLILPIVLYNGVAEWSAPTDLAELIDVVEPELEPY
                     LPRLRYKVIDEGSYSAEELRRRESLPALLFWFEKNRKREDLQWGVQGLVQRLDKPEDA
                     GLRRAFMVWVTRVLPGGRKVVEQIPEVLGLEEFQDMLEKTVLEWNEELREEGRQEGLR
                     EGLQKGLQKGEAKLLIRQLELKFGPLDEETRNRVLAAEPDRLLEWGERILKAPSLADL
                     FGD"
     gene            2909..2985
                     /locus_tag="VEL74_05425"
     tRNA            2909..2985
                     /locus_tag="VEL74_05425"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2943..2945,aa:Pro,seq:cgg)
     gene            3061..>4096
                     /locus_tag="VEL74_05430"
     CDS             3061..>4096
                     /locus_tag="VEL74_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYG61999.1"
                     /translation="MFARKLFGAVAAVLLFTGAVHAAETGAAEVRDRVRVWRQAHEKQ
                     VLQELAGLLAIPNVAAADPASIQRNAEHLQEMLRRRGFATRILKAGETSRPAVFGEMK
                     SPGATRTVVFYAHFDGQPVDPKAWTSDPWKATLRTGKLGDPATREVDIASHNGPFDGE
                     WRIYARSASDDKGPIVAMLAALDALRSAGIPPSVNLKVFLEGEEEDGSPHLTDILRAH
                     SALLDADAWVLCDGPVHQTGRAMMYYGARGVVSANFTVYGPLRALHDGHYGNWAPNPA
                     VLLTHLLAGLRDPEGKVLIPGFYEDVRPLSDTERRAAAAMPAIEDGLRRELGLARSEA
                     DGRLQDRVMLP"
BASE COUNT          658 a         1387 c         1381 g          670 t
ORIGIN      
        1 gccagctcct gctcaagaag agcatcgacg ccgccttcga ggacccgggg ctggcggtcc
       61 gcctggcgaa cctggccgtc cgggcctcgg ctcacctggg agaagcctac gatccctact
      121 gggtgatgga cctgcgggcc cgcgccctgg cgtatctcgg gaatgcccgc cgcgtcctgg
      181 gcgagctgca gagcgcggag gaggccttcc gcaaggcgga ggcctggctg gaggccagca
      241 cgacgggcaa cgtccaggtc caggccgaga tcctgggctt caagagctcc ctgcgccggg
      301 accagcgccg cttcggggag gcgctggagc tggtcgagcg ggctctccac ctttatcaag
      361 aggccgggga ctccgggggg atcgccaagt gcctcctcaa gaagtccaag atcctcgagg
      421 agaccggccg cttcgcggag gcgatcgagc tgctccgcgg cgcgcgcatc ccgcgggacc
      481 gtgagcccct cctcttcgcc tatgcccgct acaacctgct ggtctgtctc gtcctctcgg
      541 ggcagcccga agaggcggag cgcctcctgc ccgaggtccg gagcctcctc gccgagtccg
      601 ggaagcccct caacctcatc cgcctgcgct gggccgaggg gctcatcgat ctgggaatgg
      661 gccggtcggg gccggcggag gcggcgttcc gtggggtgca ggaggagttc ctggagcggg
      721 gcatgggcta cgacgccgcc ctggtctccc tcgacctcgc cttcctctat gcccgcgagg
      781 gggagacgaa ggtgctccag cacctggccc tcgagctgat gcccgtcttc acctcccgcg
      841 agatccaccg cgaggccacc gccgccctcc tcatgttcca gcacgtctgc gaggaggagc
      901 gcgccagcgc cggcctggtc cgccagctcg gcgtcttcct gcggcgcgag cggcgggggc
      961 gcggagtgta gaagcctgcg ggagcgaggc tcgttcttct cggggcaacc gttgccccac
     1021 atggaagggg cgtcattcac cccgaaacga tcgtcatccc ccatggaatg ggctccgttc
     1081 cccggggaag gtggccattt ccgaaggaat gatgtccgtt cccgctggaa tgatcgtcat
     1141 tccttggaga acgaggccgt ctccctgggg atggagtccg ttcctcgggg aatggtggcc
     1201 attcgtcggg taggaaggcc gtgcgccgaa ggacggtgga cgttcctggg gggatggtcg
     1261 cgactcctgc cggcctagtc acgaattggg acgtggcgct gcggctccgg ctatgccgct
     1321 ttgagcacga actcgacttt cacggcctcg taggggaact cgactccgcc cccctccgtc
     1381 cggctgagca agccggccgc caggagggcc gtgatgtcac catggaccgc cttcacatca
     1441 cggtcgaccc gacgggcagc ctcacggatg gagaccggtc ccacaccgca gagcgccttg
     1501 aggatctccc agcgtttcgc ggtcagcacc ttccacagca gctccggcgt cgcgaagctg
     1561 atgcgcgccg actcctgcgg ctcgcccgtc ttccaggact tcacgaaggc atccatggcc
     1621 gcggccggct ctcgaacgtc aagagtcact gttttcatcg ttccacctcg ctgatagccc
     1681 aaggagccgc ctgccgccac ttctcccttc aggggagact tcgcctgatg ggacaccatg
     1741 ttatgatagc gtgagttcgc acctgacaca tcgaatcgag gacgatctgg gtaaccacga
     1801 tctcagctac cgtctgatgt tctctcaccc gcggatgatc gaggagatgc tccgcgagtt
     1861 catcgccgag aggtgggtcg agaccctgga cttctccact ctggagaggg tcaatgccga
     1921 cttcgtcacc gaagggctca agggacgcga gggggacctc atctggaagc tccgccgcag
     1981 ggacggccgg gctgtctatc tctatctcat ggtggaattc caatccaagg tcgaccggtt
     2041 catggccgtc cgtctcatgg gctacgagag cctgctgtat caggagctca tcacccgcag
     2101 cgagctgtcc tccgacggcc gcctgcccct gatcctcccc atcgtcctct acaatggagt
     2161 ggcggagtgg tcggcgccta cggatctcgc cgagctgatc gacgtggtgg agccggaatt
     2221 ggagccctac ctcccccggc tgcggtacaa ggtcatcgac gaggggagct acagcgccga
     2281 ggagctgaga cggcgggaga gcctgccggc cctgcttttc tggttcgaga agaaccggaa
     2341 gcgcgaggat ctccagtggg gcgtgcaggg gctggtccag cgtctcgata agcctgagga
     2401 cgccgggctg cggcgggcct tcatggtatg ggtgacccgg gtcttgccag gcgggaggaa
     2461 ggtcgtcgag cagattccag aagttctcgg actggaggag ttccaagaca tgttggagaa
     2521 gacagtcctc gagtggaatg aagaattgag agaggaaggc cgtcaggagg gtcttcggga
     2581 gggtcttcag aagggccttc aaaaagggga ggcgaagctg ctcatccgcc agctcgagct
     2641 gaagtttggg cctctcgacg aggagacgcg gaaccgggtc ctcgccgcgg agccggaccg
     2701 tctgctggag tggggcgagc ggatcctcaa ggccccaagc ctcgccgatc tcttcggcga
     2761 ctgaaacggc gccatcgatc ttctggcggt gagggcttga cccccacgcg gtgggagagt
     2821 atccttcctc gtcctcgcgg cagcccttcg aggtgcgccc gctccggcgg gtggttcgag
     2881 ggggcaggac gctgcaagac aacacactcg gggcgtagct cagcttggta gagcgcacgg
     2941 ttcgggaccg tgaggccgga ggttcaaatc ctctcgcccc gaccattccc accttccaga
     3001 atgcatccgg tcttccccgg cggcgggtta agatagcccg ccttcaatag gggatcgacc
     3061 atgtttgcaa ggaagctctt cggagccgtc gccgccgtcc ttctgttcac tggcgccgtt
     3121 cacgccgccg agaccggcgc cgccgaggtc cgcgaccggg tgcgcgtctg gcggcaggcg
     3181 cacgagaagc aggtgctcca ggagctggcg ggccttctcg ccattcccaa cgtcgcggcg
     3241 gccgaccccg cctccatcca gcgcaacgcc gagcacctcc aggagatgct ccgccggcgc
     3301 ggcttcgcga cccgcatcct caaggccggc gagacgagcc gtccggcggt gttcggggag
     3361 atgaagtccc ccggcgccac ccgcaccgtc gtcttctatg cccacttcga cggccagccg
     3421 gtcgatccca aggcgtggac gtccgacccc tggaaggcga cgctgcgcac cggcaagctc
     3481 ggcgacccgg cgacccggga ggtggacatc gcctcccaca acggcccctt cgacggcgag
     3541 tggcggatct acgcccgctc ggccagcgac gacaaggggc cgatcgtcgc catgctcgcc
     3601 gccctcgacg ctctccgcag cgccggcatc cccccgtccg tcaacctcaa ggtcttcctg
     3661 gaaggagaag aggaggacgg ctcgccgcac ctgaccgaca tcctccgcgc ccactccgcc
     3721 ctcctggacg ccgacgcctg ggtgctgtgc gacgggccgg tgcaccagac cggccgggcg
     3781 atgatgtact acggcgcccg cggcgtggtg agcgccaact tcaccgtcta cgggccgttg
     3841 cgcgcgctcc acgacggcca ctacggcaac tgggccccca acccggcggt cctcctcacc
     3901 catctgctcg ccgggctgcg cgaccccgag gggaaggtgc tgatccccgg cttctacgag
     3961 gacgtgcggc cgctcagcga caccgagcgc cgggcggcgg cggccatgcc ggcgatcgag
     4021 gacggtctgc ggcgcgagct gggcctggcg cgcagcgagg cggacgggcg gctccaggac
     4081 cgggtcatgc tgccag
//
LOCUS       DASPJI010000207         5069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_186006, whole genome shotgun sequence.
ACCESSION   DASPJI010000207 DASPJI010000000
VERSION     DASPJI010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186006"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>5069
                     /locus_tag="VEL74_05435"
     CDS             <1..>5069
                     /locus_tag="VEL74_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HYG62000.1"
                     /translation="LEFLGRADQQVKIRGVRIEPGEVEATLTRHPAVREAAVTVHEEP
                     AGQRRLVAYVVPVREPAKAGSDLYRLPNGLEVACLNRNEADLIYQEIFADETYLAHGI
                     TLGDGAYVFDVGANIGLFSLFLSGRFHDLRLFAFEPIPPTFEVLRANVELYGIDAMLF
                     PCGIAERSGEAEFTFYPHWSAMSGAHADQAEEEAVSRVALVNQDHRLEEHSDDLLAAR
                     FAEKRVYTCRLRTLSEVIRETGVGRIDLLKVDAEKSEENVLAGLLDRDWERIAQLVVE
                     VHDTGGRLDRVRALLAEHGFAVVVDQDAPLRGTAIYNLYAIHPERVRPTPGDERPRPD
                     LRRALRPVLRLDELGPFLAERIPEQMVPAVFVPVERLPRMPSGKLDRAALPAPESVTP
                     PEDSFVAPRTPTEERLAAIWAEVLGLVESGRRIGIHDNFFRLGGDSIQSILVVSRAAR
                     AGLHLTARQLFENQTIAALATLVDAASGPAEPVEDGPVSGEVPLTPAQHAFFSAALPH
                     PEHFNQAVLLAVETPAPERRAGGAPLRGAVELLIHHHDALRTRFELPTPEGPAVRQVN
                     DPPGVASLPCAEVDLSALPAARRSAALEAAAASTQGSLDLAAGPLLRAVYFHLGAGEA
                     DRLLLVIHHLVVDGVSWRVLLEDLEIAWRGLATGGGADLPLRTTSFQRWAERLTAHAR
                     SATVQAEREGWQALLDRAPASLPVDTTAGPNDVASLRSVTVALDPEETRALLQEVPRV
                     YRTQINDALLTALVEAIAPWTGNRRLLLELEGHGREDVFPDLDVSRTVGWFTSAFPVL
                     LELPEAAGSGAALKAVKEQLRSQPGTGLGYGLLRHLPAADGGAAPLGAAPTPEVVFNY
                     LGQLDQTVAAGHFFPAPESAGPMRHPRQPRLALLEITAAVAKGRLQATWLYSSNRHDA
                     GTVERLATGFTAALRRLVEHCRTPDAGGFTPSDFPLARVDQTTLDRLAATAMGTAGIE
                     DLYPATPTQQGLLFHTLKSPGSGVYLGQLSLALGPELDAAAFERAWQRVVDRHAALRT
                     AFFGFDLERLLQVVQVRARLPWARHDWGGLPPAELSRRLASMVEEDRRRDFDLATPPL
                     ARVALIHLGAEGYQFVWTHHHAILDGWSMPILLRDLFTFYEAAVAGREAELEPAPAYR
                     AYIAWLEGRDPAGAETVWRRALSGVTTPTPLPGEAEGGGGGAGGGAQRRLPLSATVTN
                     ALRALARDHGLTLNTVLQGAWALLLHRASGGWEGESVVFGSVTAGRSAPVRGIEEMVG
                     LFINTLPVRVAIDAAAGLTDWLRGLQERQIEVRQFEDSPLFQVQGWSEVPRGRPLFES
                     VLAFENYPIDPSTRERAGLGLRITDVAMHEQTSYPLTLSTEPGPVLRLRLSHDARRIP
                     GPAAARLVRQLAALLAGMAADPGAALASLPVLTEEEQHQVLWEWNDTGKECPQVPLVH
                     ELFAAHAARHPDAPAISSPAGGLTYGEVEARANRLAHHLRSLGVGPDVLVAVCTDRTP
                     ERVVGIVGVLKAGGAYVTLDPTHPRERLAFLLEDARAPVLLTQQAFLEVLPETGARVL
                     CLNTDEASEWGDASRPPTSGVTPDNLSYVVYTSGSTGKPKGIEIPHAGLMNLVRWHQD
                     LYQVKPEDRGTQIASPAFDASIWELWPYLAAGASVHVPDEETRLSAAGMIRWWASQNI
                     TLAYLMTPLAEGVLAETIPEG"
BASE COUNT          698 a         1900 c         1724 g          747 t
ORIGIN      
        1 cctggagttc ctcggccgcg ccgaccagca ggtcaagatc cgcggtgtcc gtatcgagcc
       61 cggggaggtg gaagcgacgc tgacccgcca tccggcggtg cgggaggccg cagtgaccgt
      121 gcacgaggag cctgcggggc aacgccgtct ggtggcctac gtggtcccgg tgagggagcc
      181 agcgaaggcc ggcagcgacc tctaccgcct gcccaacggc ctggaggtgg cctgcctgaa
      241 ccgcaacgag gccgacctca tctaccagga gatcttcgcc gatgagacct acctcgccca
      301 cggcatcacc ctcggggacg gcgcctatgt cttcgacgtt ggggccaaca tcggcctctt
      361 cagccttttc ctgagcggac gtttccacga cctccgactg ttcgccttcg agcccatccc
      421 gccaaccttc gaggtgctgc gcgccaacgt cgaactctac gggatcgacg ccatgctctt
      481 cccctgcggg atcgcggagc gctccggcga ggccgagttc actttttatc cccactggtc
      541 ggcgatgtcc ggcgcccatg ccgatcaggc ggaggaggaa gcggtcagcc gggtcgccct
      601 agtcaaccag gatcaccggc tggaggagca ctcggacgac ctgctggccg cccgcttcgc
      661 cgagaaacgg gtctacacct gccgcctgcg caccctctcc gaggtcatcc gcgagactgg
      721 cgtcggacgc atcgacctcc tcaaggtaga cgccgagaag agcgaggaga acgtcctcgc
      781 cggtctcctc gaccgggact gggagcgcat cgcgcaactg gtcgtcgagg tgcacgacac
      841 cggaggccgg ctcgaccgcg tccgtgccct gctcgcggag cacggcttcg cggtggtcgt
      901 cgatcaggat gcgcccttgc gtgggaccgc gatctacaac ctgtacgcca tccacccgga
      961 gcgggtgcgg ccgacccccg gcgatgagcg cccgcgtccg gacctgcgcc gggctctccg
     1021 ccccgtgctg cgcctcgacg agttggggcc cttcctcgct gagcgcatcc cggaacagat
     1081 ggtgccggcg gtcttcgtcc ccgtagagcg cctgccgcgg atgccgagcg gcaagctcga
     1141 ccgtgccgcg ctccccgccc cagagagcgt cacaccgccg gaggacagct tcgttgcgcc
     1201 gcgcaccccc accgaggagc ggctcgccgc catctgggcc gaagtgttgg gcctggtcga
     1261 gagcggccgg cgcatcggca tccacgataa cttcttccgc ctcggcggcg attcgatcca
     1321 gagtatcctg gtggtctccc gcgccgcccg ggcggggttg catctgaccg cccggcagct
     1381 gttcgagaac cagaccatcg ccgccctggc gaccctggtg gacgctgcca gcggcccggc
     1441 ggagccggtt gaagacggcc cggtgagcgg cgaggtgccg ctgactcccg cccaacatgc
     1501 cttcttctcc gcggcgctcc cccaccccga gcacttcaat caggcggtgc tgctggcggt
     1561 ggagaccccc gcgccggaac gacgcgccgg cggcgctccg ttgagaggcg ccgtggagct
     1621 cctgatccac catcacgacg ccctgcgcac gcgcttcgag ctacccacgc cggagggccc
     1681 ggccgtgcgg caggtcaatg atccgccggg agtcgcctct ctcccctgcg ccgaggtcga
     1741 tctctcggcg ctcccggcgg cacggcggag cgcggcgctg gaggccgcag ccgcctccac
     1801 ccaggggagc ctggacctcg ccgccggccc gctgctccgc gcggtctact ttcatctcgg
     1861 ggcaggcgag gcggaccgtc tgctgctggt gatccaccac ctggtggtcg acggcgtctc
     1921 ctggcgcgtc ctgctcgagg atctggagat cgcctggcgc gggctggcga cgggcggcgg
     1981 cgcggacctg ccactgcgca ccacctcgtt ccaacgctgg gccgaaaggc tcaccgcgca
     2041 cgcacgctcg gcgacagtgc aagcggagcg ggaggggtgg caggcgctcc tcgaccgcgc
     2101 ccccgcgtcg ctccccgtcg acaccaccgc gggtcccaac gacgtggcct cgctacgtag
     2161 cgtcaccgtg gccctcgatc cggaggagac gcgcgccctc ctccaggagg tgccgcgggt
     2221 ctatcgcacg cagatcaacg acgcgctgct caccgctctc gtggaagcca tagcaccctg
     2281 gaccgggaac cgccgcctgc tcttggagct cgaagggcat ggacgggagg acgtcttccc
     2341 cgatctggac gtgtcacgta ccgtcggctg gttcaccagc gcatttccgg tgctgctcga
     2401 gctgccggag gccgccggtt ccggcgcggc tctgaaagcg gtcaaggagc agctccggtc
     2461 acagcctggc accggcctcg gctacggcct gctgcgccat ctccccgcgg ctgacggcgg
     2521 ggccgcgccc ttgggcgccg cgccgacgcc cgaggtcgtc ttcaactatc tcggacagct
     2581 cgaccagacg gtggccgccg ggcatttctt tccggctcct gaatcggccg gcccgatgcg
     2641 ccatccgcgg caaccgcggc tcgcacttct ggagatcacc gccgccgtcg ccaaggggcg
     2701 gctccaggcg acctggttgt acagctccaa ccgtcacgac gccggcactg tcgagcggct
     2761 ggccactggc ttcaccgccg ccctgcgccg actcgtggag cactgccgga cgcccgacgc
     2821 cggcgggttc acgccgtcgg acttcccgct cgcccgcgtg gaccagacga ccctcgaccg
     2881 gctcgcggcc accgcgatgg gcaccgcggg gatcgaggac ctctacccgg ccaccccgac
     2941 gcagcagggg ctgctctttc acaccctgaa atcccccggc tccggcgtct acctcggaca
     3001 gctcagcctc gccctcggcc cggagctgga cgccgccgcc ttcgagcgcg cctggcagcg
     3061 ggtggtggac cgtcacgccg ccctgcgcac cgcctttttc ggcttcgatc tggagcgcct
     3121 gctgcaggtc gtgcaggtcc gtgcgcggct cccctgggca cgccacgact gggggggcct
     3181 tccccccgcc gagctgagcc gccggctcgc ctccatggtg gaggaggatc ggcggcggga
     3241 cttcgacctc gccacgcccc cgttggcacg cgtcgccctg atccacctcg gcgccgaggg
     3301 gtaccaattc gtctggaccc atcaccatgc catcctcgac ggctggtcca tgcccatcct
     3361 gctgcgcgac ctcttcacct tctacgaggc cgccgtagcc gggcgggagg cggagctgga
     3421 gccggcccct gcctaccgcg cctacatcgc ctggctggag gggcgggatc cggcaggggc
     3481 ggagacggtc tggcgtcgcg ccctctccgg tgtcaccacc cctacccccc tgcctgggga
     3541 ggcggaggga gggggaggcg gcgcgggcgg cggagcgcag cggcgcctcc ccctgtccgc
     3601 cacggtcacc aacgccctgc gcgccctcgc ccgcgaccac gggctgacgc tcaacaccgt
     3661 gctccagggg gcctgggcgc tcctcctgca ccgcgccagc ggcggctggg agggcgagag
     3721 cgtggtcttc ggcagcgtca ccgccggacg gtcggcgccg gtgcgcggta tcgaggagat
     3781 ggtcggcctg ttcatcaata ccctgccggt gcgggtcgcc atcgatgccg cggcagggct
     3841 gaccgactgg ctgcgcggcc ttcaggagcg ccagatcgag gtgcgacagt tcgaggacag
     3901 cccgctcttc caggtgcagg gctggagcga ggtgccgcgc ggccggcctc tcttcgagag
     3961 cgtcctcgcc ttcgagaact atccgatcga cccgtcgacg cgcgagcgcg ccgggctcgg
     4021 cctccgcatc accgacgtcg cgatgcacga gcagaccagc tatccgctca ccctgagcac
     4081 ggagccgggc cccgtgctgc gtctgcgcct ctcccatgac gcgcgacgga tcccgggacc
     4141 ggcggcggcc cgtctcgtgc gccagctcgc cgcgctcctg gccggcatgg cggccgatcc
     4201 cggcgcggcg ctcgcctccc ttcccgtact gaccgaggag gagcagcacc aggtcctctg
     4261 ggagtggaac gacaccggca aggagtgtcc gcaggtgccg ctggtccatg agctgttcgc
     4321 ggcccatgcc gcgcggcatc cggacgcacc cgccatctcc tcgcccgcgg gcgggctgac
     4381 gtatggcgag gtcgaggcgc gggccaaccg cctggcccac catctacgct ccctcggtgt
     4441 cggaccggac gtgctggtgg cggtgtgcac cgaccgcacc ccggagcggg tggtcggcat
     4501 cgtcggcgtg ctcaaggcag gcggcgccta cgtcacgctg gaccccaccc atccgcggga
     4561 gcggctggct ttcctgctgg aagacgcccg ggcgccggtc ctcctgaccc aacaggcgtt
     4621 cctagaggtg ctgcccgaga cgggcgcccg ggtgctctgt ctcaacacgg atgaggcgtc
     4681 ggagtggggg gacgcgtccc ggccgccgac gagcggcgtc acaccggaca acctgtcgta
     4741 cgtcgtctac acctcgggct ccacgggcaa gcccaagggg atcgagatcc cgcacgccgg
     4801 gctgatgaac ctggtgcgct ggcatcagga cctctatcag gtgaagcccg aagaccgcgg
     4861 cacgcagatc gcgagcccgg ccttcgacgc ctccatctgg gagctgtggc cgtacctcgc
     4921 ggccggagcg agcgtccacg tgccggacga ggagacgcgg ctttccgcgg ccggcatgat
     4981 ccgctggtgg gccagccaga acatcaccct ggcctacctg atgaccccgc tcgccgaagg
     5041 ggtgctcgcg gagacgattc cggagggtc
//
LOCUS       DASPJI010000208         3957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_413808, whole genome shotgun sequence.
ACCESSION   DASPJI010000208 DASPJI010000000
VERSION     DASPJI010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413808"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1209)
                     /locus_tag="VEL74_05440"
     CDS             complement(184..1209)
                     /locus_tag="VEL74_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HYG62001.1"
                     /translation="MRLSALLLLPLLLVAAVAYGQASNEKADVILSTAAAPASPAWAL
                     AIHGGAGTIPKSMGAAEKDAYLKSMSAALEAGRQVLASGGTSLDAVEKVVRFLEDDPL
                     FNAGKGAVFTHDGTHELDAAIMNGRDLSCGSVAGLKTVKNPISLARRVMERSPHVFMV
                     GEGAEAFAAEMGVERVDPKYFFTQRRWDQLQEALRKEKEAAAGAPKGIGTVGATALDR
                     HGNLAAATSTGGMTNKRFGRLGDVPVIGAGTYANNRTCAISATGWGEKFIRNTVAHDI
                     SALMEYGGLTLQQAADRVIHQKLDKGDGGIVGVGRDGSIALVFNSEGMYRGAADSNGR
                     FEVKIWE"
     gene            complement(1478..3082)
                     /locus_tag="VEL74_05445"
     CDS             complement(1478..3082)
                     /locus_tag="VEL74_05445"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HYG62002.1"
                     /translation="MEGGLSTPGIPPEAWNELLALSRDESGDRVLVERGLDLWRRVCG
                     ADAAAIYLDKGGFLEREVACGTALPETADPAAIPAAFGVRPFPGGRLLFAPASAAPPP
                     GSEPLGHALTALLASSFKGCRLRQELKEQQFQVNYRVVELEALYDVGLAVTATLDLDR
                     LSEEILLRAVSLLDARRGALYILEDGHYRLDRTFGGQAAPLFAADDAEVRAFLTAGGA
                     PPADLLPGARYVLGAPIEVESGARGLLAVGDKESRRGVGPFLTSDRRTLALFANQAAI
                     ALDNARLHREALEKERLEREMQLAAEIQRRILPTGAPQLSGFKLVGWYRPARQVGGDY
                     YDLFRVDENRVGMVVGDVSGKGIPAALMVSTLHSALRLLLDRTGLGPDLLEQLNRHIL
                     ESSTPNKFITMLLGELDVRSGVVHYLNAGHNPGLLLRGSGSVEELGSSGLPLGLLPMA
                     RFQSRFISLDPGDVVCLYSDGITECSAPDGEEFGTERLAGLMREYRERPLQEMLEAIR
                     DATDCFAAGLPQGDDQTLVLLRRDTL"
     gene            complement(3093..3443)
                     /locus_tag="VEL74_05450"
     CDS             complement(3093..3443)
                     /locus_tag="VEL74_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYG62003.1"
                     /translation="MTESLNVTVDRRDGLAVIYTEGYINNQGGEEIARVAYDLLDAGY
                     RILLLNLGGTKIVNSIGISILIEIIEKMIEVEGRMGFCCLTPTIEKTFQIMGLAQYAP
                     IFPDESAAVSQLKP"
     gene            complement(3457..3873)
                     /locus_tag="VEL74_05455"
     CDS             complement(3457..3873)
                     /locus_tag="VEL74_05455"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG62004.1"
                     /translation="MREVTLALPMMRDMEIEASKTATAIAEAMHMTPDRIDEVRMAVI
                     EACINAFEHSHAQDQEVFVTFQVLGEGDPEKLRITVRDHGVGFNPGQMEEPRIEKKLT
                     AKDKRGWGLKIIRGLMDEVEIHSDSGGTTVVMSKLR"
BASE COUNT          577 a         1364 c         1399 g          617 t
ORIGIN      
        1 cccgacctgg tctgggagga cagcctggcg agcgtgctct cccgggtgcg ggcgatcggc
       61 gccgacgccg tgcccatcgc cacccgcgag ggcttcctcg gcgccatcac cgccccgctg
      121 ctcgccgtcg acgcgggcga cgaggtcggc gacctccgcc cgcaccgcgg ataggtcccc
      181 cgcctactcc cagatcttga cctcgaagcg gccgttcgag tcggccgcgc cgcggtacat
      241 cccctcggag ttgaagacca gcgcgatcga gccgtcgcgc ccgacgccga cgatgccgcc
      301 gtcccccttg tcgagcttct ggtggatgac ccggtccgcc gcctgctgca gggtgaggcc
      361 gccgtactcc atgagcgccg agatgtcgtg cgccacggtg ttgcggatga acttctcgcc
      421 ccagccggtg gccgagatgg cgcaggtgcg gttgttggca taggtgccgg cgccgatcac
      481 cggcacgtcg ccgagacggc cgaagcgctt gttggtcatg ccgccggtgg aggtggcggc
      541 ggccaggttg ccgtgccggt cgagagcggt cgcccccacc gtgccgatcc ccttgggagc
      601 ccccgccgcc gcctccttct ccttgcgcag cgcctcctgg agctggtccc agcggcgctg
      661 ggtgaagaag tacttgggat cgacccgctc gacccccatc tcggcggcga acgcctcggc
      721 cccctcgccc accatgaaga cgtgggggga gcgctccatc accctgcgcg ccagcgagat
      781 ggggttcttg accgtcttga gccccgccac cgagccgcac gagaggtcgc gcccgttcat
      841 gatggcggcg tcgagctcgt gggtgccgtc gtgggtgaag accgccccct tgccggcgtt
      901 gaacaggggg tcgtcctcga ggaaccggac caccttctcc accgcgtcga ggctggtgcc
      961 gccggacgcg agcacctggc ggccggcctc gagcgccgcc gacatcgact tgagataggc
     1021 gtccttctcc gccgcgccca tcgacttcgg gatcgtcccc gcgccgccat ggatggcgag
     1081 cgcccacgcc ggggacgccg gggccgcggc cgtgctgagg atgacgtcgg ccttctcgtt
     1141 ggatgcctgg ccgtaggcga cggcggcgac gaggagcagg gggagcagga ggagtgcgga
     1201 gagtctcatg aaggggattc tagaggagga ccgccgccgc ggatgcgaga ggaagctcct
     1261 ggaatcgatc cggacgccgc ggatctcgtc cagacgccgc ggatggcatc cggacgctct
     1321 ggatgcttcc cgagcgctgc ggatctcgtc cagctctccc ggatggagcc tggagccttc
     1381 ggatgcttcc cgagcgtccc ggatgcgatc caatccaaaa agatggattc cggagggccc
     1441 ggaagcttcc cggacgctcc ggatatgcag gcaggggcta gagggtgtcg cgccgcagca
     1501 gcaccagcgt ctggtcgtcc ccctggggga ggccggcggc gaagcagtcg gtggcgtcgc
     1561 ggatggcttc gagcatctcc tgcagggggc gctcgcggta ctcgcgcatc agacccgcca
     1621 gacgctcggt gccgaactcc tcgccgtcgg gggcgctgca ctcggtgatg ccgtcgctgt
     1681 agaggcagac gacgtcgccc ggatcgaggg agatgaagcg cgactggaag cgggccatcg
     1741 gcaagaggcc cagcggcagg ccgctcgagc ccagctcctc cacgctcccc gatccccgca
     1801 gcagcaggcc ggggttgtgc ccggcgttca ggtagtgcac gacgccggag cgcacgtcca
     1861 gctcgcccag gagcatggtg atgaacttgt tgggggtgct ggattccagg atgtgccggt
     1921 tgagctgctc gaggaggtcc gggccgaggc cggtgcggtc gaggagcagg cgcagcgccg
     1981 agtggagcgt cgacaccatg agggcggccg ggatcccctt gcccgagacg tcccccacca
     2041 ccatgccgac gcggttctcg tccacccgga agaggtcgta gtaatccccg cctacctgcc
     2101 gggcggggcg gtaccagccg acgagcttga agccggagag ctgcggcgcc ccggtgggca
     2161 ggatgcggcg ctggatctcg gcggcgagct gcatctcgcg ctccagccgc tccttctcca
     2221 gcgcctcgcg gtggaggcgg gcgttgtcca gggcgatggc ggcctggttg gcgaacagcg
     2281 ccagggtgcg ccggtcggag gtgaggaacg gccccacgcc gcggcggctc tccttgtccc
     2341 cgaccgccag caggccgcgg gcgccgctct cgacctcgat cggagcgccc agcacatagc
     2401 gggccccggg caggaggtcg gccgggggcg cgccgccagc ggtgaggaag gcgcgcacct
     2461 cggcgtcgtc cgccgcgaac aggggggcgg cctggccgcc gaaggtgcgg tcgagacggt
     2521 agtggccatc ctccaggatg tagagggcgc cgcggcgcgc gtcgaggagc gagacggcgc
     2581 gcagcaggat ctcctccgac aggcggtcga ggtcgagggt ggcggtcacc gcgagcccca
     2641 cgtcgtagag cgcttcgagc tccaccaccc ggtagttgac ctggaactgc tgctccttga
     2701 gctcctggcg gagccggcaa cccttgaagg aggaggccag cagagcggtg agcgcgtggc
     2761 ccaggggctc cgagccgggc ggcggcgcgg ccgaggcggg ggcgaagagg aggcgcccgc
     2821 cggggaacgg gcggacgccg aaggcggcgg ggatcgccgc cgggtccgcg gtctccggca
     2881 gcgcggtgcc gcacgccacc tcccgctcca ggaagccccc cttgtccagg tagatcgcgg
     2941 cggcatcggc gccgcagacc cggcgccaga ggtcgaggcc gcgctcgacc agcacgcggt
     3001 cccccgactc gtcgcgggag agcgcgagca gctcgttcca ggcttccggc gggattccgg
     3061 gggtggagag gccgccctcc atcaccccca cctcagggct tgagttggga gacggcggcc
     3121 gattcgtcgg ggaagatcgg tgcatactgg gccaacccca tgatctggaa ggtcttctcg
     3181 atcgttggcg tcagacagca gaatcccatg cgcccctcta cctcgatcat cttctcgatg
     3241 atttcgatca ggatcgagat cccgatggag ttgacgatct tcgtcccgcc caggttgagg
     3301 agcaggatgc ggtagcccgc gtcgaggagg tcgtaggcca cccgcgcgat ctcctcgccc
     3361 ccctggttgt tgatgtagcc ctccgtatag atgacggcca ggccgtcccg gcggtccacg
     3421 gtcacgttga ggctctcggt catctcatcc ctgccttcat cgcaacttgc tcatcaccac
     3481 agtcgtcccg ccggagtcgg agtgtatctc cacttcgtcc atgagcccgc ggatgatctt
     3541 gagcccccag ccacgcttgt ccttggcggt gagcttcttc tcgatcctcg gctcctccat
     3601 ctggccgggg ttgaagccga cgccgtggtc ccgcacggtg atgcggagct tctccggatc
     3661 cccctcgccc agcacctgga acgtcacgaa gacctcctgg tcctgggcgt ggctgtgctc
     3721 gaaggcgttg atgcaggcct cgatcaccgc catccggacc tcgtcgattc tatccggagt
     3781 catgtgcatg gcctcagcga tggcggtcgc cgtcttgctc gcttcgatct ccatgtcgcg
     3841 catcatcggg agcgccagcg tgacctccct catgaccgcc tcctcgcctg ccatgtccgc
     3901 acgtcgctcg tagaagaagt ctcagaaaga gaggtacagc tgcagcgcgt agatcac
//
LOCUS       DASPJI010000209        17808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_413811, whole genome shotgun sequence.
ACCESSION   DASPJI010000209 DASPJI010000000
VERSION     DASPJI010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17808)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17808
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413811"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3449
                     /locus_tag="VEL74_05460"
     CDS             <1..3449
                     /locus_tag="VEL74_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62005.1"
                     /translation="RLARRLGRRGKAPAGEEGGAAEPRQVPLLVASLGPGYLFAGALV
                     AVLYLVARLVEPLLASSLELSPGLSAWQFLFLGYRPELAWYLPLDRFPFLAGLLTVFL
                     WLTLWWLAGMGLRLAAGGTLGRNLADHRTDEAVLPAWRRWAGAPFLASPVASYLEWAG
                     WLLAVAAPLLIWAWLSLGGDPYRVRPSEVAVALVLWTSWTLHLLLRGIDQKPDAAPEE
                     APAEKVEAAGWPEVLARLEQELGMAPPEPLALLPVEPLGRSEVDPRVAALLSPLVAAL
                     LPGARQLTPMQRVVLTRLALQGYVHTDPPVALDELRLGQSVRDAVEDKSGQRSRNTIV
                     LAPEGAGKTLLALLAAANHALVHTRATLLVVRGEGEAEALAERFRQTLEPSTLRWNVR
                     VRLLGGDLMNDLAQGILPDVMVASLRDLVVMVLDRSDTFAPFLRTVGLVLVDDVESFA
                     GPVEVHAQLAFRRLTLRLRELTGVRELGEESAPQVLVLGCESMHRMADWVRSLCGIDA
                     VVRNFHHSAREAKAREAAELAAGGIATASKEEGVAEETGEPAAEALQKVRHQRLYRLR
                     DFTGATGEPLSAAALVAVCERLAVPWHYRLCGDGRRDLGRGPLRLEEEPVHGTASAEE
                     ACVVLLDGEWSEVRREIQRLRRAGARFRRWRGAGAPSGETVEAEPEPIALLTCTDPDL
                     DEALGAAASGPVASRELAELPRPVIRPPTGLAVEPHLSADLVQHWIEVEDLLQVFGSS
                     LASRLRDLARGGLLLCERRTDVDERANRYVERVHVRALARAVRSEDGDGPGGSSAPLP
                     PKVVQVEFAVRETVALRDRTNLTELGETGAGSAHLLYYPGRVFQDARGIFVVVGRASE
                     SGGRGEPAGDVLVEPLLLDEVSAPRRVHLIEELAPENAPPELAEAAGGSFPPADLVLL
                     GRYPLRVALLPVEVRVEHLATYRLDPVRRQVRQRTLLQNETRERYRRLVLPTVALAIL
                     PNPAEAAGQEGEESHRGPVLTLAGARLVAAAMRVLMPSLYRGGAAGLQVALRLDDPLH
                     AEADQPLGPGEGFYLFDADEGGNGMARALHRDGVEVLLRLCRRLLDRVASYDHLRALH
                     DEWASEAEILEESRTDPRFGGESPEALERRRARDEQARDVALAWLDSRLSPEAGAGGG
                     AR"
     gene            3494..4561
                     /locus_tag="VEL74_05465"
     CDS             3494..4561
                     /locus_tag="VEL74_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62006.1"
                     /translation="MLGLAAGCGQEPPVPRELPFPVIETAAPFPELAVPAAPAAGPAQ
                     ESAPRSTFEAALTATPPPDPAATPQPPAAPAGLSVPLGGQLLGELPPDGSWRWSAEGG
                     AVLAVHASGGTRPTALIYAETFTDLVRDRPSEEHQRFRVTVDPAAAEGHLPTGEPLRR
                     VAQRTGLSGTEAARFLQLVTTRTGGRGLGFRPAPESFTGWRWVGRNEHGVTLRLARQS
                     GSWAEPPSLPARFAEALARLNGSHGAAPGASSGGAAGAVPAYLILGSVTDRYEETGVH
                     LAVLCARSPACAEARDLAAFLASIQPAEAGRLERLAAAPSAGFADLARELGVEILPAD
                     QVPTREGLTAGGQSPPAGAAR"
     gene            4607..5527
                     /locus_tag="VEL74_05470"
     CDS             4607..5527
                     /locus_tag="VEL74_05470"
                     /inference="COORDINATES: protein motif:HMM:NF036774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HYG62007.1"
                     /translation="MPTRKPSIHIALLALAVGLSGLGTREARAYGEQIQGEARKLARE
                     IAKANLGTVAVVDFSDPSGNVMELGRFLAEEMSGELASARKGFEVIDRSKLKSLLREH
                     KLATTGLLDPQTTREMGKIAGVNALVTGTLTRLATTVRLRLNVLDIETAKIETSSAVD
                     IPETDQIKALLRREVEEPMDEPVLDVQASAARAKEANGVRFTLPSCRRARKKVRCDLL
                     ATSLHQEKELALTLESRAIDRSGNEYPADLVQIGQREGETAKNRLLVGTPLRVSIHFS
                     GVPTNLRAFQVMDLDFQSFSVRFEDVAIAD"
     gene            6435..8180
                     /locus_tag="VEL74_05475"
     CDS             6435..8180
                     /locus_tag="VEL74_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HYG62008.1"
                     /translation="MAVKDQEPTVLTAETKAAPADSIYKDDALDTFAEARQRWEKAKA
                     KSEGQVPFWKRDFTTVSGMEVPHLVTPEQVADIDVRKDIGIPGEYPYTRGVHPTGYRG
                     KLWTMRQFAGFGTAADTNQRFKYLLSQGQTGLSVAFHLPTLYGYDSDHKMSTGEVGKC
                     GVTVDTLADMEILFEGIPLGEVTTSMTINSTAPILFAMYLAVAEKQGVDIQRQVSGTL
                     QNDILKEYIAQKEYIYPPRPSMRLITDQFAFAAKHVPRWNTISISGYHIREAGSTALQ
                     ELAFTLRDGMEYVQYGIDAGLDVDEFAPRLSFFFNSHNDFFEEIAKFRAARQIWATVM
                     RERYGAKNPRSWMLRFHTQTAGCSLTAQQPYNNVVRTTVQALAAVLGGTQSLHTNALD
                     ETLALPTEENARIALRTQQILAHESGVINTVDPLGGSWFVEEMTRRMVDGAFDYIRRI
                     DELGGMVEAVEAGFPQREIMDAAFAYQRAYEEKEKLIVGLNAFQVENEKPIDILYISD
                     DLAERQIGFLNDVKSRRDAGAVERTLDALKRGAAGSENTMPLILDCVKAYCTVGEISD
                     AMREVFGTYEEPAVF"
     gene            8220..8729
                     /locus_tag="VEL74_05480"
     CDS             8220..8729
                     /locus_tag="VEL74_05480"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYG62009.1"
                     /translation="MSIPAAAARELLLYMLWADRHMLAAVRPVQPEHLTRDAGVSFGS
                     LLGTMVHMLGSQRVWLSRFSGNPLDRRPSAEDYPDLPAWIHGWEETASGIEAFLAALT
                     DAQLAAPLTWTSFSGDTYTQPLWQLVIHLTNHSSYHRGQVVSLLRQMGYQSPSTDLSY
                     YFIERSTAS"
     gene            8794..10080
                     /locus_tag="VEL74_05485"
     CDS             8794..10080
                     /locus_tag="VEL74_05485"
                     /inference="COORDINATES: protein motif:HMM:NF036398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62010.1"
                     /translation="MKTENAAAVRPPSWRAAGTAALLAGGLLLGQAMQAEAQGVVTVT
                     QMVVSNCVMEATCEWKVSCRAGNGPETQLADNWKATVGTMMDVGKTLDLPAVPATLSC
                     KLEEDDGWFGTTWEDAGSATLELQGGGDFEFNVGNEEEGQVILKLRVATIEVHEPAPA
                     APAATTATGRAASRTAAAAPAKARLYVGTFAPKESGHAAVIGLPWEQFKARADALAAT
                     GTKPVWMGSYEEGGTRYWSGIFHYYDGDYQIVHGLEWEPFFAKWKQLTNREGQRLTDF
                     EMYNEKGKVLCTGLYELGSDKHNLRIGLARDEFVNEVNRMSARGLRLVDVEVYSMQGR
                     TLYAGAFREMEGGYALAAGLAWDPFMERYKKVSGEGNSFMDLETYVEGGRRLWDGSIT
                     GLSGQITEPLDAKAFAARWKEMAAKGLHLVSVETYR"
     gene            10147..10548
                     /locus_tag="VEL74_05490"
     CDS             10147..10548
                     /locus_tag="VEL74_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HYG62011.1"
                     /translation="MSDRRIRILIAKPGLDGHDRGAKVVARALRDAGYEVIYTGLRQT
                     PEQIAAAAVQEDVDAVGLSILSGAHNSLLPEVTRLLREQGAEDILVFAGGIIPEQDIA
                     GLKGSGVDEIFLPGTSTKAIVDYLQNRLVAA"
     gene            10636..11949
                     /locus_tag="VEL74_05495"
     CDS             10636..11949
                     /locus_tag="VEL74_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG62012.1"
                     /translation="MSRRILLADDSVTIQKVIELTFMDEDFEVRAVSNGDEALAALPQ
                     FRPEFVIADVHMPGANGYEVCRRAKQQLPEVPVLLLVGTFEPFDEAQARGAGADSFLK
                     KPFDSQELLQRVQDLMASRAATAPAAPAATPTIPAMAVVPPAAPAPPAIDEDVFQLPS
                     IDLGPDPEPAAPRAATPAYSEPEAQWPAFELEPEPEPVPMPAVAPAPEPFQEEQPFSF
                     REERSAGFEAPGFGAGFGGAQPVDVPAQPFAPEDPFTLEGDASLTLEEPPIGLREESD
                     EPAPFSYGEPQDRFLEDAERPILETMEASAAAPEPWSLTDQERLGIPLGQEPLVDSMA
                     ETEERIASPWPDALEAAEGVAPHEPAATFSAPAVAAAAYAPAASEAPANGSGRLSDED
                     VDRIARRVVELLGDKAVRDIAWEVVPDLAEVVIKDRLRELESQVD"
     gene            12176..14863
                     /locus_tag="VEL74_05500"
     CDS             12176..14863
                     /locus_tag="VEL74_05500"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HYG62013.1"
                     /translation="MDKRFDPQSYEGAWQRRWAEMGVFRVEVPSGKPPFCIMIPPPNV
                     TGKLHVGHALQSTLQDLLTRWRRMQGYNALWLPGTDHAGIATQLMVERHLVTEGTSRR
                     ELGRAAFLERMWAWKEQYHGNIRSQLDLLGASCDWSRERFTLDEGLSRAVREAFVRLY
                     NEGLITRGEYMVNWSPTLGTAVSDLEVETKTVQGKLYHIAYPVEGSDPENGERIVIAT
                     TRPETMLGDTGVAYHPEDERYRHLAGKAAVLPLVGRRLPFVADTVVDKEFGTGLVKLT
                     PFHDPNDFEMGRRHGLPGISVIGLDGRMTAEAGPDFAGLDRFEARAKVVERLREQGFL
                     VKVEEHTHNVGHSQRSGEPIEPLVSTQWFCDVSGMARQALDAVRDGRLVLKPESWVKT
                     WEHWLENIKPWCISRQLWWGHQIPAWYTEDGRCFVARDAAEAAALAGTDRLTQDPDVL
                     DTWFSSALWPFSTLGWPEETPDLRTFYPTDVLITGFDILFFWVARMVMTGLHFTGDVP
                     FRSVHLTGLVRDAEGVKMSKTKGNVLDPADLVAEYGADAVRFTLASLDSPGRDIPLNP
                     EQIAGYRAFGNKIWNAARFALSRTQELPDARVRPEIDPEGLAAPERWILSRLSRVAAE
                     IDPRFETFRFDEACNRLYHFFWGDLCDWYIELSKPALSGEAPRPRVGEVLMTVLDRSL
                     RLLHPIMPFLTEEVWQRLPGHEVIHPETICLAPYPRAEAAWEDPEAEAGMDVLIQVVT
                     RVRGLRAELGLPPKAKFDLHLSAAAAADAAIGRFLDEQAPLIAFLCRAEHVVSGAAPE
                     GTRRDLVAGVEVALAVERPELSPEERQRLAKELEKLAADIARAEERLSNEGFLAKAPA
                     HVVEGGRAKLAEMRERQASLGASLGDSASLGDSASLGQS"
     gene            14873..15706
                     /locus_tag="VEL74_05505"
     CDS             14873..15706
                     /locus_tag="VEL74_05505"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HYG62014.1"
                     /translation="MSEAEFQRYLREIERLRPVGTGTGAGRGPENLIVLERVASTNGL
                     ARSIAVEYEKEGQDLEPLLILAFEQSGGRGRQGRQWMSPRGQGVYATRVLSVTDPEML
                     QTLPLLVGVGLCRALGAHLGSPCRLKWPNDLLVETGGGRRKIGGILIEALVRPGEGAA
                     AFIGFGVNHGHSVEVLPTGATSLHLERAGQAAGAGLEALTWDLVAGLEAELSHLGDVG
                     YAVNSYRSWSVHQPGERLTCRVGDSVAEGTFAGFDDAGRLLLRTSEGEVRLSAGEVIE
                     P"
     gene            15739..16503
                     /locus_tag="VEL74_05510"
     CDS             15739..16503
                     /locus_tag="VEL74_05510"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009226308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HYG62015.1"
                     /translation="MLILVDVGNTNTVFGVYRGDELVESFRLSTDTERTADEYGSLLL
                     PLFALAGIEPTAADAVVVSSVVPPLHFTLDSLARRYFGKKPLFIEPGVRTGMPIRYDN
                     PAEVGADRIVNAVAARERYGAPVIVVDFGTATTFDLVNAAGEYVGGIIAPGITISAEA
                     LFAHASRLYRVDVRKPDELVGRNTAGAMQAGIYYGYIGLVDGILERLLEEIPETKTVV
                     ATGGQADLIAGGSRFIREVDPMLTLIGLKLIYDRNR"
     gene            16500..17159
                     /locus_tag="VEL74_05515"
     CDS             16500..17159
                     /locus_tag="VEL74_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62016.1"
                     /translation="MTEPGGEDDHAYFQAIEEVFVQLRGAPLLLSPADWQVARRWHRE
                     GVPLDLVRRALEEVFEKRKERGTKGKISSLRYCAPAVEAAWNDLRELTAPGEREEAPS
                     FDIPARLAALAAALPAGIPGREGYQLRIRTLEGGPQAVEESLAALDLEVLEAGGAALD
                     EAARAEVEGAVTATLASLGSRLPVAELARSRERLTRQILRQRLGLPVLSLFSPEAERA
                     G"
     gene            complement(17160..17639)
                     /locus_tag="VEL74_05520"
     CDS             complement(17160..17639)
                     /locus_tag="VEL74_05520"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYG62017.1"
                     /translation="MRFSYAVHIQAPMEEVFALLTDPEKTGLWTEGLEENVFPEGYDR
                     EHPVGTRFRQAIRESDLLSLYEGEVTAYERPGLYGVRLVNQRLAVDVVYRLAPDRNGT
                     RLVYSADVRHASWLERLATRLFSHLTEGVIRNQMRRLKQVVESGGQPVPPPQIDFGW"
BASE COUNT         2594 a         6306 c         6339 g         2569 t
ORIGIN      
        1 cccggctggc gcggcggctc gggcgccgcg gcaaggcgcc ggcgggggag gagggcgggg
       61 cggcggagcc gcggcaggtg ccgctgctgg tggcgagcct cggccccggc tacctgttcg
      121 ccggcgccct cgtggccgtc ctctatctcg tagcgcgcct ggtcgagccg ctcctcgcct
      181 cctccctgga gctgtcgccg gggctctcgg cctggcagtt cctcttcctg ggctaccggc
      241 cggagctcgc ctggtacctg cctctggacc gcttcccctt cctcgccggc ctgctgacgg
      301 tgttcctctg gctgaccctc tggtggctgg ccgggatggg cctgcgcctg gcggctggcg
      361 gcaccctggg gcgcaacctg gcggaccacc ggaccgacga agcggtgctg ccggcctggc
      421 ggcgctgggc gggggccccc ttcctcgcct cccccgtggc ctcgtacctc gaatgggccg
      481 gctggctcct ggccgtggcg gcccctctcc tgatctgggc ctggctctcc ctcggcggcg
      541 acccctaccg cgtgcgcccc tccgaggtcg ccgtggccct ggtcctctgg acctcatgga
      601 cgctgcacct gctgttgcgc ggcatcgacc agaagcccga cgcggcaccc gaggaggcac
      661 cggcggagaa ggtggaggcc gccggctggc ccgaggtgct cgcccgcctc gagcaggagc
      721 tgggcatggc gccgcccgag ccgctggcgc ttctgccggt ggagcccctg ggccggtccg
      781 aggtggaccc ccgggtggcg gccctcctgt cgccgctggt cgccgcgctc ctgcccgggg
      841 cgcgccagct cacccccatg cagcgcgtgg tgctgacgcg gctggcgctc cagggctacg
      901 tccataccga tccgccggtg gccctggacg agctccgcct cggccagtcg gtgcgcgacg
      961 ccgtggagga caagagcggc cagcgcagcc gcaataccat cgtcctggcg ccggaagggg
     1021 cgggcaagac cctgctcgcc ctcctcgccg ccgccaacca cgctctggtc cacacccggg
     1081 ccactctcct ggtggtccgc ggcgagggag aggccgaggc gctggcggag cgcttccgcc
     1141 agaccctgga gccctccacc ctgcgctgga acgtgcgcgt gcgcctcctg ggcggcgacc
     1201 tgatgaacga cctggcgcag gggatcctgc ccgacgtcat ggtggccagc ctgcgcgacc
     1261 tggtggtcat ggtcctcgac cgctccgaca ccttcgcgcc gttcctgcgc acggtcggcc
     1321 tggtcctggt ggacgacgtc gagtccttcg cgggcccggt ggaggtgcac gcccagctcg
     1381 ccttccgccg cctgaccctg cgcctgcgcg agctcaccgg cgtccgcgag ctgggggagg
     1441 agagcgcgcc gcaggtcctg gtcctgggct gcgagagcat gcaccggatg gccgactggg
     1501 tccgcagcct gtgcggcatc gacgccgtcg tgcgcaactt ccatcactcg gccagggagg
     1561 cgaaggcgcg ggaggcggcc gagctggccg ccggcggcat cgccacggcg tccaaggagg
     1621 aaggcgtggc ggaggagacc ggcgagccgg cggccgaggc ccttcagaag gtccgtcacc
     1681 agcggctcta caggctgcgc gatttcaccg gcgccaccgg cgagccgctc tccgccgccg
     1741 ccctggtggc ggtctgcgag cgcctggccg ttccctggca ctaccggctc tgcggcgacg
     1801 ggcggcgcga cctcggccgc ggtccgctgc gcctggagga ggagccggtc cacggcaccg
     1861 cgtcggcgga ggaggcctgc gtcgtcctgc tggacggcga gtggagcgag gtgcggcggg
     1921 agatccagcg gttgcggcgg gcgggggcgc gcttccggcg ctggcgcggc gccggcgctc
     1981 cctccggcga gacggtggag gcggagcccg agcccatcgc cctcctcacc tgcaccgatc
     2041 cggacctgga cgaggccctg ggcgcggcgg cttccggccc ggtggcgtcc cgcgagttgg
     2101 cggagctgcc gcggccggtg atccgccccc ccaccgggct cgccgtggag ccccatctct
     2161 cggccgacct ggtgcagcac tggatcgagg tggaggacct cctccaggtc tttggcagct
     2221 ccctggcctc caggctgcgg gacctcgccc gcggcggcct gctcctctgc gagcggcgga
     2281 ccgacgtgga cgagcgggcg aaccgttacg tggagcgggt gcacgtccgc gccctggccc
     2341 gggcggtgcg ttcggaggac ggcgacggcc ccggcggctc gtcggcgccg ctccccccca
     2401 aagtggtaca ggtggagttc gccgtgcgcg agaccgtggc cctccgcgac cgcaccaacc
     2461 tgaccgagct gggggagacc ggagccggct ccgcccatct cctctactat cccggccgcg
     2521 tcttccagga cgcccgcggc atcttcgtgg tggtgggccg ggcctccgaa tcgggtgggc
     2581 gcggcgagcc ggcgggggat gtcctggtgg agccgctgct cctggacgag gtctcggccc
     2641 cccggcgcgt ccacctcatc gaggagctag cgcccgagaa cgcccctccc gagctggcag
     2701 aagccgccgg cggctccttc cccccggccg acctggtcct cctgggccgc tacccgctgc
     2761 gggtggctct gctgccggtg gaggtccgcg tcgagcacct cgccacctac cggctggacc
     2821 ccgtccgccg tcaggtccgc cagcgcaccc tgctgcagaa cgagacgcgg gagcgctacc
     2881 gccggctcgt cctcccaaca gtcgccctgg cgatcctccc gaatccggcc gaggcggccg
     2941 gccaggaggg ggaggagtcc cataggggtc cggtcctcac cctggcgggg gcgcgcctcg
     3001 tcgccgcggc gatgcgggta ttgatgccct ccctataccg tggcggcgcc gccggcctgc
     3061 aggtggccct acggctggac gacccgctgc acgcggaggc ggatcaaccc ctggggccgg
     3121 gggagggctt ctacctcttc gacgccgacg aggggggcaa cggcatggcg cgggcgctgc
     3181 accgcgacgg cgtggaggtg ctgctgcggc tctgccggcg tctgctggac cgcgtcgcct
     3241 cctacgacca cctgcgggcg ctccacgacg agtgggcgag cgaagccgag atcctggagg
     3301 agagccggac cgatccccgc ttcggcggcg agtcgccgga ggccctcgag cggcgccgcg
     3361 cgcgggacga gcaggcgcgg gacgtggccc tcgcctggct ggacagccgc ctgtcccccg
     3421 aggcaggggc cggaggcggt gcgcgatgaa cagcaggaga ccggtcccgt ggcgtgggtt
     3481 gggagctctg ctgatgctgg gccttgccgc gggctgcggt caggagccgc cggtgccgcg
     3541 ggagctgccc ttcccggtca tcgagaccgc ggcgcccttc ccggagctgg cggtgccggc
     3601 ggcgcccgcc gccggacccg cccaggagag cgcgccccgg agtaccttcg aggcggcgct
     3661 gacggcgacc ccgccgcccg atcccgccgc cacaccccag cctccagccg ctcccgcggg
     3721 gctgagcgtt cctctcggcg gccagctcct gggcgagctg ccgccggacg gctcctggcg
     3781 ctggtccgcg gaagggggcg cggtgctcgc cgtccacgcg tccggcggca cccggcccac
     3841 ggcgttgatc tatgccgaga cgttcacgga cctggtccgc gaccgtcctt cggaggagca
     3901 tcagcggttc cgggtaacgg tggaccccgc cgcggccgag gggcaccttc ccacgggcga
     3961 gccgctgcgg agggtggcgc agcgcacggg gctgtcgggg accgaggccg cccgcttcct
     4021 gcaactcgtg accacccgca cgggggggcg cggcctcggt ttccgtccgg cgccggagag
     4081 cttcaccggc tggcgctggg tgggccgcaa cgagcacggc gtgacgctgc ggctcgcaag
     4141 gcagtccggc tcctgggcgg agccgccatc cctgccggcg cgcttcgccg aggccctggc
     4201 ccggctgaac ggatcgcacg gcgcggcgcc gggggcctcc tccgggggag ccgccggcgc
     4261 cgtccccgcc tatctcatcc tgggcagcgt caccgaccgc tacgaggaga cgggcgtcca
     4321 cttggccgtg ctctgtgccc gctccccggc ctgtgccgag gcccgggacc tggcggcctt
     4381 cctggcctcc atccagcccg ccgaggccgg tcgcctcgag cgcctcgccg ccgcgccctc
     4441 cgctgggttc gccgacctgg cgcgggagct gggtgtcgag atcctgccgg cggaccaggt
     4501 gccgacgcgg gaggggttga cagcgggggg gcagagtccg ccagccggtg ccgcccggta
     4561 gcacggtcgc cggaacacag ccatggaacg aatgggaggg tccgcgatgc cgacgagaaa
     4621 accgtccatc cacatcgcgc tgctcgccct ggccgtcggg ctctcgggcc ttggcacccg
     4681 ggaggcccgg gcttacgggg agcagatcca gggggaggcg aggaagctgg ccagggagat
     4741 cgccaaggcg aacctcggga ccgtggccgt ggtcgacttc agcgacccgt cgggaaacgt
     4801 catggagctt gggcgtttcc tggccgagga gatgtcgggc gagctggcct cggcgcgcaa
     4861 aggcttcgag gtgatcgacc ggtcgaagct caagagcctg ctgcgggagc acaagctggc
     4921 gaccaccggc ctgctcgatc cccagacgac gcgcgagatg ggaaagatcg ccggcgtgaa
     4981 cgccttggtg accggcaccc tgacccgcct ggccaccacc gtgcgcctgc ggctcaacgt
     5041 cctggacatc gagaccgcga agatcgagac ctcctccgcc gtcgacatcc ccgagaccga
     5101 ccagatcaag gcactcctgc gccgggaggt cgaggagccc atggacgagc cggtcctgga
     5161 cgtccaggcg tccgcggcgc gggcgaagga agcgaacggg gtccgcttca ccctgccgag
     5221 ttgcaggcgc gccaggaaga aggtccgttg cgacctcctc gccaccagcc tgcatcagga
     5281 aaaggagctg gcgttgaccc tggagtcccg ggccatcgat cgatcgggga acgaataccc
     5341 agccgacctg gtccagatcg ggcagagaga aggggagacc gcgaagaacc ggcttctggt
     5401 gggcacgccg ctccgcgttt ccatccattt ctcgggagtg cctacgaacc ttcgggcctt
     5461 ccaggtcatg gacctcgact tccagagctt ctccgtccgc ttcgaggacg tggctatagc
     5521 cgactgatca ggaagcgaag cacgtccagg caccgcgccc ctggggattc gaggcgaagc
     5581 tccgaggacg ttcctcactg ttgaggcatc gtccctcagt gttgagggcc gatctcgtgg
     5641 cgccgaggga ccgcggcgaa gtgctgaggg atgatccctc aataccgagg cgctgatccg
     5701 gagcactccg gggcaatccc tcagtgttga ggagccgctc cgaagcattg aggggttgtt
     5761 cctcaacgct gagggcgcac tcctcaaccc tccggcgcga tgcctcaata ctccgaagtc
     5821 atccctcagt gttgaggaag cgcggcgaag tgttgaggaa gcgcggcgaa gtgttgagga
     5881 gtcgcggcga agtgttgagg aacggccccg aagcactgag gagcggcccc gaagtgttga
     5941 ggaacggccg gggagtgttg aggagccgcc gcgaagcatt gaggaacggc ggcgaagtgt
     6001 tgagggatgg tgcctcagca agagggactc gccgaaaagg gagggcatcc tctgaaaaag
     6061 tgaggccctc tccgtgaaga accggcggag agcgtcggag gaccgctctc ttgtgcggct
     6121 aggccctggg tggaggtctc aactccatcc ggggccgctc gccgcgggtt gaaggaccgg
     6181 acgaagaggt attcctcgcc cggggacagc ctgaaaagaa cggtgctgcg gtacggcttg
     6241 acgaccgagg acgactttat ctccgtgaaa tcggggcgtc aagaggtttg agggaatggg
     6301 gcgcgtgccc acggagttgg actccctggg ccggcgaccc ggtccctccc tcctcccctg
     6361 gagggcctcc ccccatcgtc tatactatgg ggctcgacgg cggtgccgtc cgatcccgaa
     6421 aggagcccag aggaatggcc gtcaaggacc aggagccgac cgtgctgacc gcggagacca
     6481 aggccgcgcc cgcggactct atctataagg acgacgccct cgacaccttc gccgaggcgc
     6541 gccagcgctg ggagaaggcc aaggccaagt ccgaggggca ggtgcccttc tggaagcgcg
     6601 acttcaccac cgtctccggc atggaggtcc cgcatctggt gacccccgag caggtcgccg
     6661 acatcgacgt ccgcaaggac atcgggatcc cgggcgagta cccctatacg cgcggcgtcc
     6721 acccgaccgg ctaccgcggc aagctctgga ccatgcggca gttcgccggc ttcggcaccg
     6781 ccgccgacac caaccagcgc ttcaagtacc tgctctccca ggggcagacc ggcctgtccg
     6841 tcgccttcca cctgcccacc ctctacggct acgactccga ccacaagatg tcgaccggcg
     6901 aggtggggaa gtgcggcgtc accgtcgaca ccctggcgga catggagatc ctcttcgagg
     6961 gcatccccct gggcgaggtc accacctcca tgaccatcaa ctccaccgcc ccgatcctct
     7021 tcgccatgta cctggcggtg gccgagaagc agggggtgga catccagcgc caggtctccg
     7081 ggacgctgca gaacgacatc ctcaaggagt acatcgcgca gaaggagtac atctatccgc
     7141 cgcggccgtc gatgcggctg atcacggacc agttcgcctt cgcggcgaag cacgtgccgc
     7201 gctggaacac catttccatc tcgggctacc acatccgcga ggccggctcg accgccctgc
     7261 aggagctggc gttcaccctg cgcgacggca tggagtacgt gcagtacggc atcgacgccg
     7321 gcctggacgt cgacgagttc gcgccgcggc tgtcgttctt cttcaacagc cacaacgact
     7381 tcttcgagga gatcgccaag ttccgcgccg cccgccagat ctgggcgacg gtgatgcgcg
     7441 agcgctacgg cgccaagaac ccgcgctcct ggatgctgcg cttccacacc cagacggcgg
     7501 gctgctccct caccgcccag cagccgtaca acaacgtcgt gcggaccacc gtccaggcgc
     7561 tcgccgcggt gctcggcggc acccagtcgc tgcataccaa tgcgctcgac gagacgctcg
     7621 ccctgccgac cgaggagaac gcccgcatcg ccctgcgcac gcagcagatc ctcgcccacg
     7681 agtcgggggt catcaacacc gtcgacccgc tcggcggcag ctggttcgtc gaggagatga
     7741 cccgccgcat ggtggacggc gcgttcgact acatccgccg catcgacgag ctcggcggca
     7801 tggtggaggc ggtcgaggcc ggcttcccgc agcgcgagat catggacgcc gccttcgcct
     7861 atcagcgggc gtacgaggag aaggagaagc tgatcgtcgg cctcaacgcc ttccaggtgg
     7921 agaacgagaa gccgatcgac atcctctaca tctccgatga cctcgccgag cgccagatcg
     7981 gcttcctgaa cgacgtcaag agccggcgcg acgcgggcgc ggtggagcgc accctcgacg
     8041 cgttgaagcg cggcgcggcg gggagcgaga acaccatgcc gctcatcctc gactgcgtga
     8101 aggcctactg caccgtcggc gagatcagcg acgcgatgcg cgaggtcttc ggcacctacg
     8161 aggagccggc ggtcttctag gccttgactt gatttctctt ctgttcggga ggcgatgaca
     8221 tgtcgatacc ggccgcagcc gcgcgggagc tgctccttta catgctctgg gccgaccgcc
     8281 acatgctggc ggcggtgcgg cccgtccagc ccgagcacct gacgcgcgac gccggggtga
     8341 gcttcggcag cctcctcggc accatggtgc acatgctggg ctcccagcgg gtgtggctct
     8401 cccgcttctc cggcaacccg ctggaccgcc ggccgtccgc ggaggactat ccggacctcc
     8461 ccgcctggat ccacggctgg gaggagacgg cctccgggat cgaggccttc ctcgccgccc
     8521 tgaccgacgc gcagctcgcc gcccccctca cctggaccag cttcagcggc gacacctaca
     8581 cccagccgct ctggcagctg gtgatccacc tgaccaatca cagctcctat caccgcggcc
     8641 aggtggtgtc gctgctgcgg cagatgggct accagtcccc gtcgaccgac ctctcctact
     8701 acttcatcga acgctccacg gcctcgtagc gctccctttt tcccctcatg tgacctacca
     8761 ttggaaccgc cacatctcag aagggggaca gccatgaaga ccgagaacgc tgcggcggta
     8821 cgtccgccgt cctggcgcgc cgccgggacg gccgcgctcc tggcgggtgg gttgctgctg
     8881 ggccaggcga tgcaagctga ggcgcagggg gtggtcaccg tcactcagat ggtggtcagc
     8941 aactgcgtga tggaggcgac ctgcgagtgg aaggtctcct gccgggccgg caacggcccc
     9001 gagacccagc tcgccgacaa ctggaaggcc accgtcggga ccatgatgga cgtggggaag
     9061 acgctcgacc tccccgccgt cccggctacc ctcagctgca agctggagga ggacgacggc
     9121 tggttcggca ccacctggga ggacgccggc agcgccaccc tggagctgca ggggggcggc
     9181 gacttcgagt tcaacgtcgg caacgaggag gagggccagg tgatcctcaa gctccgcgtc
     9241 gccaccatcg aggtccacga gccggccccc gccgcccccg ccgctacgac ggccaccggc
     9301 cgggcggcct ccaggacggc ggcggcggct ccggcgaagg cgcgcctcta cgtcggcacc
     9361 ttcgcgccga aggagagcgg ccacgccgcg gtgatcggcc tgccctggga gcagttcaag
     9421 gcccgcgccg acgccctggc cgcgacgggc accaagcccg tctggatggg ctcctatgaa
     9481 gaggggggca cgcgctactg gagcggcatc ttccactact acgacggcga ctaccagatc
     9541 gtccacggcc tcgagtggga gccgttcttc gccaagtgga agcagctcac caaccgcgag
     9601 gggcagcggc tcaccgactt cgagatgtac aacgagaagg ggaaggtcct ctgcaccggc
     9661 ctctacgagc tgggcagcga caagcacaac ctccggatcg gcttggcgcg cgacgagttc
     9721 gtcaacgagg tgaaccggat gtccgcccgc ggcctgcggc tggtcgacgt ggaggtctac
     9781 agcatgcagg gtcggacgct ctatgccggc gccttccgcg agatggaggg gggctacgcc
     9841 ctggcggcgg gcctcgcctg ggaccccttc atggagcggt acaagaaggt gtccggcgag
     9901 ggcaacagct tcatggacct cgagacctac gtcgaagggg ggcgccgcct ctgggacggc
     9961 tccatcaccg gcctctcggg gcagatcacg gagccgctcg acgccaaggc gttcgccgcc
    10021 cgctggaagg agatggccgc caaggggctc catctcgtca gcgtcgagac ctatcggtga
    10081 acctcgcggg agacggggag gtgcccatcg agcgcctccc cctcccctct gctacaatcc
    10141 cgccgcatga gtgaccgaag gattcgcatc ctgatcgcca agccgggcct ggacggccac
    10201 gatcgcgggg cgaaggtcgt ggcgcgcgcc ctgcgcgacg ccgggtacga ggtgatctac
    10261 accgggctgc gccagacgcc ggagcagatc gccgccgccg ccgtccagga ggacgtggac
    10321 gccgtcggcc tgtccatcct ctccggcgcc cacaacagcc tgctgcccga ggtgacgcgg
    10381 ctgctgcggg agcagggggc cgaagatatc ctggtctttg ccgggggcat catcccggag
    10441 caggacatcg ccggcctcaa gggctccggg gtggacgaga tcttcttgcc cggcacgtcc
    10501 accaaggcca tcgtcgacta tctacagaat cgtctcgtgg cggcctgagc gcctccctaa
    10561 atcgctggtg gagaacggtt tgacggcttg acgccggggg tgctaccgta cccggtgcaa
    10621 cgttcggagg ggttcatgag cagacggatt ctgctggccg atgacagcgt caccatccag
    10681 aaggtcatcg agctgacctt catggacgaa gacttcgagg tgcgggcggt cagcaatggt
    10741 gacgaggcgc tcgcggccct gccgcagttc cggcccgagt ttgtcatcgc cgacgtccac
    10801 atgcccggcg ccaacggcta cgaggtctgc cggcgcgcca agcagcagct cccggaggtg
    10861 ccggtcctgc tgctggtggg caccttcgag ccgttcgacg aggcgcaggc gcgcggcgcc
    10921 ggcgccgact cgttcctcaa gaagccgttc gactcccagg agctgctgca gcgcgtccag
    10981 gatctgatgg cttccagggc ggccaccgct ccggccgctc cggcggccac gcccaccatc
    11041 cccgcgatgg cggtcgtgcc tcctgcggcc ccggcgcccc cggcgatcga cgaggacgtg
    11101 ttccagctcc cgtccatcga cctcggaccg gacccggagc ctgctgctcc tcgggccgcc
    11161 accccggcct acagcgagcc ggaggcgcag tggccggcct tcgagctgga gcccgagccg
    11221 gagcccgttc cgatgccggc cgtggctccc gcgccggagc cgttccagga ggagcagccg
    11281 ttctcgttcc gcgaggagcg ctccgccggc ttcgaagccc ccggtttcgg tgccgggttc
    11341 ggtggggcgc agcccgtcga cgtgccggcg cagccgtttg cccctgaaga ccccttcacc
    11401 ctggagggcg acgcctcgct gaccctggaa gagcctccca tcggtctgcg ggaggagtcg
    11461 gacgagccgg cgcccttctc ctacggcgag ccccaggacc ggttcctcga agacgccgag
    11521 cgcccgattc tggagacgat ggaggcctcc gcggccgccc cggagccctg gagccttacc
    11581 gatcaggagc gcctcgggat ccccctgggc caggagccgc tggtcgacag catggccgag
    11641 accgaggagc ggatcgcctc cccctggccg gacgccctgg aggcggccga aggggtggca
    11701 ccccacgagc cggccgcgac cttcagcgcg ccggccgtcg ccgcggccgc ctatgccccc
    11761 gccgcttccg aggccccggc gaacggctcc ggccggctga gcgacgagga cgtggaccgg
    11821 atcgcgcggc gcgtcgtcga gctgctcggc gacaaggccg tccgcgacat cgcttgggag
    11881 gtcgtcccgg acctcgccga ggtcgtcatc aaggaccgcc tccgcgagct cgagagccag
    11941 gtcgactgag cgagtcccct ttcgttcgag cggcccggga gctccgggcc ttcccccagc
    12001 ggcacggccc gggttcccgg cagtacaacg ttacgacggg ggagcggtat gatccgctcc
    12061 cccgctcgtc agagagcgga cgacggcccg ggttcctccg gccggaggtg gcagaaccat
    12121 ggagacggcg gtgagcggta cgagcatccc tgagacagag aagacggcgg cagagatgga
    12181 caagcgtttc gatccgcaga gctacgaggg cgcctggcag cggcgttggg ccgagatggg
    12241 cgtcttccgg gtcgaggtcc cctccggcaa gccgccgttc tgcatcatga tcccgccgcc
    12301 caacgtcacc gggaagctgc acgtcggcca cgcgctccag tccacgctcc aggacctgct
    12361 gacgcgctgg cgccggatgc agggctacaa cgccctctgg ctgccgggga ccgaccacgc
    12421 cggcatcgcc acccagctga tggtggagcg ccacctcgtc accgagggga ccagccggcg
    12481 cgagctcggc cgcgccgcgt tcctcgagcg gatgtgggcc tggaaggagc agtaccacgg
    12541 caatatccgc agccagctcg acctgctcgg cgcgagctgc gactggagcc gcgagcgctt
    12601 caccctcgac gaggggctct cccgcgccgt gcgcgaggcg ttcgtccgcc tttacaacga
    12661 ggggctgatc acccgcggcg agtacatggt gaactggtcg cccacgctcg gcaccgcggt
    12721 ctccgacctg gaggtggaga cgaagaccgt ccagggcaag ctctaccaca tcgcctatcc
    12781 ggtggagggg agcgacccgg agaacggcga gcgcatcgtc atcgccacca cccgccccga
    12841 gaccatgctg ggagacaccg gcgtcgccta tcacccggag gacgagcgct accgtcacct
    12901 ggcgggcaag gccgcggtcc tgccgctggt ggggcggcgc ctgcccttcg tggccgacac
    12961 ggtggtcgac aaggagttcg gcaccggtct ggtcaagctg acgcccttcc acgatcccaa
    13021 cgatttcgag atggggcggc gccacggcct gccgggcatc tcggtgatcg gcctcgacgg
    13081 ccgcatgacc gccgaggcgg ggccggactt cgccggcctc gaccgcttcg aggcgcgggc
    13141 caaggtggtg gagcgcctgc gcgagcaggg cttcctggtg aaggtggagg agcataccca
    13201 caacgtcggc cacagccagc gcagcggcga gccgatcgag ccgctggtct cgacgcagtg
    13261 gttctgcgac gtgtcgggca tggcccgcca ggcgctggac gcggtgcgcg acggccgtct
    13321 cgtgttgaag cccgagagct gggtcaagac ctgggagcac tggctcgaga acatcaagcc
    13381 ttggtgcatc tcgcggcagc tctggtgggg gcaccagatc ccggcctggt acaccgagga
    13441 cggccgctgc ttcgtcgccc gcgacgccgc ggaggccgcg gcgctggcgg ggaccgaccg
    13501 cctcacccag gacccggacg tgctcgacac ctggttctcg tcggcgctct ggccgttctc
    13561 caccctcggc tggcccgagg agacgccgga cctgcggacc ttctatccca ccgacgtgct
    13621 gatcaccggc ttcgacatcc tcttcttctg ggtggcgcgg atggtgatga ccgggctcca
    13681 cttcaccggc gacgtcccct tccgctccgt gcacctcacc ggcctggtgc gcgacgccga
    13741 gggggtgaag atgtcgaaga ccaaggggaa cgtgctcgac ccagccgacc tggtggccga
    13801 gtacggcgcc gacgcggtgc gcttcaccct ggcgtcgctc gacagcccgg ggcgggatat
    13861 cccgctcaac cctgagcaga tcgccggcta ccgcgctttc ggcaacaaga tctggaacgc
    13921 ggcgcgcttc gcgctgtcgc ggacgcagga gctgccggat gctcgggtgc ggcccgagat
    13981 cgatccggag gggctggcgg cgcccgagcg ctggatcctg tcgcggctct cgcgggtggc
    14041 ggcggagatc gatccgcgct tcgagacgtt ccgcttcgac gaggcgtgca accgcctgta
    14101 ccacttcttc tggggcgacc tctgcgactg gtacatcgag ctgtcgaagc cggcgctctc
    14161 cggcgaggcg ccgcggccgc gggtggggga ggtgctgatg acggtcctcg accgcagcct
    14221 gcgcctgctc catccgatca tgccgttcct caccgaggag gtctggcagc ggctgcccgg
    14281 gcatgaggtg atccatcccg agaccatctg cctcgctccc tacccgcggg ccgaggcggc
    14341 atgggaggac cccgaggccg aggcgggcat ggacgtgctg atccaggtgg tcacccgggt
    14401 gcgcgggctg cgtgccgagc tcggcctgcc gcccaaagcg aagttcgacc tccacctttc
    14461 ggcggcggcc gccgcggacg ccgccatcgg ccgcttcctc gacgagcagg cgccgctgat
    14521 cgccttcctc tgccgcgccg agcacgtggt gtccggcgcc gcccccgagg ggacccggcg
    14581 cgacctggtg gctggggtgg aggtggccct ggccgtggag cggccggagc tgagcccgga
    14641 ggagcggcag cgtctcgcca aggagctgga gaagctggcg gccgacatcg cgcgggccga
    14701 ggagcggctc tcgaacgaag gcttcctcgc caaggccccg gcgcacgtcg tcgagggggg
    14761 gcgcgccaag ctggcggaga tgcgggagcg gcaggcgagc ctcggcgcca gcctgggaga
    14821 cagcgccagc ctgggagaca gcgccagtct ggggcagagc tgaggggaac ccttgagcga
    14881 agctgaattc cagcgctacc tccgggagat cgagcgcctg cgcccggtgg gcaccgggac
    14941 cggggccggc agggggccgg agaacctgat cgtcctcgag cgggtggcgt cgacgaacgg
    15001 gctcgcccgg tccatcgcgg tggagtacga gaaggagggg caggacctcg agccgctgct
    15061 gatcctggcc ttcgagcagt ccggaggccg tggacgccag ggccggcagt ggatgagccc
    15121 gcgcggccag ggggtctatg ccacccgggt gctgtcggtg accgaccccg agatgctcca
    15181 gaccctcccc ctgctcgtgg gcgtggggct ctgccgggcg ctgggggccc acctcggctc
    15241 cccctgccgg ctcaagtggc ccaacgacct gctggtggaa actggcggcg ggcgccgcaa
    15301 gatcgggggg atcctgatcg aggccctggt ccgtccggga gagggggccg cggccttcat
    15361 cggcttcggc gtcaatcacg gccactcagt ggaggtgctg cccaccgggg cgacgtcgct
    15421 ccacctggaa cgggccggcc aggcggccgg cgccggtctc gaggcgctga cctgggacct
    15481 ggtcgccggg ctggaagcgg agctgtccca cctgggggat gtgggctacg ccgtgaacag
    15541 ctaccggagc tggtcggtcc accagcccgg agagcggctg acctgccggg tcggcgacag
    15601 cgtggccgag gggaccttcg ccggtttcga cgacgccggc cggttgctgc tccgcacctc
    15661 cgagggggag gtgcgtctct cggcggggga ggtcatcgag ccatgagcag gggacgcagg
    15721 aaggccgcgg cggacgagct gctgatcctg gtcgacgtcg gcaacaccaa cacggtattc
    15781 ggcgtctacc ggggggacga gctggtggag agcttccggc tgtccaccga cacggagcgc
    15841 accgccgacg agtacggctc gctcctcctg ccgctgttcg cgctcgccgg gatcgagccc
    15901 acggcggcgg acgcggtggt cgtgtcgtcc gtcgtgccgc cgctccactt caccctcgac
    15961 agcctggccc gccgctactt cggcaagaag ccgctgttca tcgagccggg ggtgcgcacc
    16021 ggcatgccga tccggtacga caacccggcg gaggtcgggg ccgaccgcat cgtcaacgcg
    16081 gtggcggcgc gggagcgcta cggcgcgccg gtgatcgtgg tcgacttcgg caccgccacc
    16141 accttcgacc tggtcaacgc cgccggggag tacgtggggg ggatcatcgc cccgggcatc
    16201 accatctcgg ccgaggcgct gttcgcccac gcctcccgcc tctaccgggt cgacgtccgc
    16261 aagccggacg agctggtcgg ccgcaacacc gccggcgcca tgcaggcggg catctactat
    16321 ggctacatcg ggctggtcga cggcatcctc gagcggctgc tcgaggagat cccggagacg
    16381 aagacggtgg tggcgaccgg cggccaggcc gatctgatcg ccggcggctc gcgcttcatc
    16441 cgtgaggtcg accccatgct cacactgatc ggcctgaagc tgatctacga ccggaaccga
    16501 tgactgagcc gggcggcgag gacgaccacg cctacttcca ggccatcgag gaggtcttcg
    16561 tccagctccg gggggcgccg ctgctgctgt cgccggcgga ctggcaggtg gctcggcgct
    16621 ggcaccgcga gggggtgccg ctcgatctgg tgcggcgggc actggaggag gtcttcgaga
    16681 agcgcaagga gcgcgggacc aaggggaaga tctcgagcct ccgctactgc gctcccgccg
    16741 tcgaggccgc ctggaacgac ctgcgggagc tgaccgcccc tggtgagagg gaggaggcgc
    16801 cgtcgttcga cattccggcc cgcctcgccg ccctggccgc cgccctgccg gccgggattc
    16861 ccgggcgcga gggctatcag ctccgcatac gaaccctgga gggagggccg caggcggtcg
    16921 aggagagcct ggcggcgctc gacctcgagg tgctggaggc cggcggcgcc gccctggacg
    16981 aggcggcgcg ggccgaagtg gagggcgccg tgaccgcgac cctggcatcc ctgggctccc
    17041 ggctgcccgt cgcggagctc gcccgctccc gcgagcggct cacccggcag atcctgcggc
    17101 agcggctggg actgccggtc ctctcgctct tctcgcccga agccgagaga gctggatagt
    17161 caccagccga aatcgatctg ggggggcggc acaggttggc cgccgctctc caccacctgc
    17221 ttgagccgtc gcatctggtt gcgaatgacc ccctcggtga ggtggctgaa gaggcgggtg
    17281 gccagccgct cgagccaact cgcgtggcgc acatcggccg aatacacgag ccgggtcccg
    17341 ttgcggtcgg gcgcgagccg gtagaccaca tcgaccgcga gccgctggtt gacgaggcga
    17401 acgccgtaga ggcccggcct ctcgtacgcg gtcacctccc cttcgtagag gctcagcagg
    17461 tcggattccc ggatggcctg ccggaagcgg gtgcccaccg gatgctcgcg gtcgtatccc
    17521 tccggaaaga cgttctcctc caggccctcc gtccagagcc cggtcttctc cgggtcggtg
    17581 agtagcgcga agacctcctc catgggggcc tggatgtgca cggcatagct gaatctcaac
    17641 gggtctccct ccggtctctc ctgaggtcta tgcaggcgcg cgcattatag tcgttgcagg
    17701 gcaacgaaaa gtgcaagatt ttttctccgc tttccgtcct ttacgggcat ggaccttcgc
    17761 catgcaccca tgctccttcc cgccggggcc tttgccgccg gttccctc
//
LOCUS       DASPJI010000210         4473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_186094, whole genome shotgun sequence.
ACCESSION   DASPJI010000210 DASPJI010000000
VERSION     DASPJI010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186094"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VEL74_05525"
     CDS             complement(<1..587)
                     /locus_tag="VEL74_05525"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vms1/Ankzf1 family peptidyl-tRNA hydrolase"
                     /protein_id="HYG62018.1"
                     /translation="MFNNIDLRELAAASGPERAFMSLYLSTPESLEGLKPRIEKIRGL
                     LADNAVELEYFEENLKLAEKWLAEHTWTTDGAAIFVCWALDFVRAYPLTVPGQDFLWV
                     DSSPYIRPLAELQDEYENFVVVAADNTASRVFFVTSARADQDARIKGDVKNNVKKGGW
                     SQKRYERRRNNELMHYSKEIADVLNEIEQKEKFDRL"
     gene            974..1321
                     /locus_tag="VEL74_05530"
     CDS             974..1321
                     /locus_tag="VEL74_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62019.1"
                     /translation="MPDHGDRTPSRVDLSRPTEATRLVLYELVVHVYFHPTPEEVEKA
                     QADPGNHWVPDYGPDEARLHVFYAYGRWFAVWEALEETKETGLPEWRRWSVVRIVEDR
                     DPSSATGVTFLEV"
     gene            1447..1677
                     /locus_tag="VEL74_05535"
     CDS             1447..1677
                     /locus_tag="VEL74_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62020.1"
                     /translation="MAVIPSEMASISRDMAVVSSGTAFISRETAALSRETSAVSRERA
                     FISEGIAGVSREMTTVSEGMAAVSQETVIALP"
     gene            1776..2696
                     /locus_tag="VEL74_05540"
     CDS             1776..2696
                     /locus_tag="VEL74_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG62021.1"
                     /translation="MSTQNQVVAELRRATRRYGKVTALDGVDLEVRAGEVVALLGPNG
                     AGKTTAVQLLLGLIRPTAGSARLFGLDPRDTAARVRVGAMLQISKVPETLKVREHVEL
                     VSSYYPRPLPLGESLAAAGLTEVGDRLFGKLSGGQRQRVLFALALCGNPDVLFLDEPT
                     VGLDVESRRAFWETVRARARAGCTVFLTTHYLEEADALASRIVLVDRGRIVAEGTPAE
                     IKARVRGRKIRCRTRLAVAELEALPGVRGVRRDGEVTEMLAAEAEPVLREMLARDPGL
                     SDLEVLGAGLEEAFLALTGNTRSTDTEVAA"
     gene            2693..3484
                     /locus_tag="VEL74_05545"
     CDS             2693..3484
                     /locus_tag="VEL74_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG62022.1"
                     /translation="MSTQTMAAGSRSLGRIYTLEAKSELLKLLRLPMYAVPTIAFPMV
                     FYLLFGVAMGGRSAGGVSYATYLMVSYGAFGVVGASLFGFGVGLAVERGQGWMLFKRA
                     TPMPPLVHLAGRLTVCLLFSAAVVLGLFALGAALAGVRLPAAAWLTLVPVLLSGALAF
                     GAFGLAIGYWVGPNSAPAVVNVIYLPVAFASGMWFPIQAMPRFVQELAPFLPSYHYTQ
                     LALKVIGADLGGAVATHVAALVGFTVVSLFLARAGFRRDEGRTFG"
     gene            3509..>4473
                     /locus_tag="VEL74_05550"
     CDS             3509..>4473
                     /locus_tag="VEL74_05550"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HYG62023.1"
                     /translation="MRFLPPKDPELGWTPYAWLIYLGFFLLKPVLGGASPIEWGATLS
                     ALGVFLGVYFWGFRQWGPRIVWAAAMIAALGFLCVPFNPGASVFWIYAGAFLGYSGPP
                     RFALRWLALLLVLLGLQSWWLDLRPSSWITAVVFSVMICGVNLHFSERTRANAKLRAT
                     QEEVERLARTVERERIARDLHDLLGHTLSVITLKAELAGKLLNRDPERAAEEIHEVER
                     ISRDALREVREAVVGYRSEGLKAELARARLALESSGIAFEYLLTPLDVDPARETVLAL
                     AVREAVTNVIRHAGARSCRIAVEQGAGGVVRLEVRDDGRGGLAAEG"
BASE COUNT          617 a         1529 c         1562 g          765 t
ORIGIN      
        1 aggcggtcga acttctcctt ctgctcgatc tcgttgagga cgtcggcgat ctccttggag
       61 tagtgcatca gctcgttgtt gcggcgccgc tcatagcgct tctgagacca cccccccttc
      121 ttgacgttgt tcttgacgtc ccccttgatg cgggcgtcct gatcggcgcg cgccgaggtg
      181 acgaagaaga cgcgcgacgc ggtgttgtcc gccgccacca cgacgaagtt ctcgtactcg
      241 tcctggagct cggcgagcgg ccggatgtag ggggaggagt cgacccagag gaagtcctgg
      301 ccgggaacgg tcagcggata ggcgcggacg aagtcgaggg cccagcagac aaagatggcg
      361 gcgccgtcgg tggtccaggt gtgctcggcg agccacttct cggcgagctt caggttctcc
      421 tcgaagtact ccagctcgac agcattgtcc gccaggagcc cccggatctt ctcgatccgc
      481 ggcttcaacc cttcgaggga ctcgggcgtg gacaggtaca ggctcatgaa cgcgcgctcg
      541 ggcccggagg ccgcggcgag ctcgcgcaga tcgatgttgt tgaacaaggg cgtttcctcc
      601 aaagaggcgc cggagatcgg cggctcgact ctggaggggg gcgggtagca aggagtgggc
      661 caggggggta tttgtccgag gcaggccgtc tcaggcggtc ttcgaggtcc cgaatttccc
      721 tcgcccgtca tcccgatttc cctcttgacg cgcatccact cgcgggtata gcctcggtgg
      781 cggtcgtaag ccgtaccagg aaggaggtgg cagggagagg agaaggacgc cgcagcttca
      841 cagcacagcc gtacaggacc gctgtgtctc cagggtgagc gtgtagctca tccggatgaa
      901 cagggtccaa gcggccaagg gagggagcag cgaactcacc tcccgaggcc ttgccgagca
      961 gaggagtgaa ctcatgcccg accacggtga tcgtacgcct tcgcgcgtcg atctgtctcg
     1021 tcccaccgaa gccacccgcc tcgttctcta tgagctggtg gtccacgtct acttccaccc
     1081 cactcctgaa gaggtcgaga aggcccaggc ggaccccggc aaccattggg tgccggacta
     1141 cggtcccgac gaggcgcgtc tccacgtctt ctacgcctac ggccggtggt tcgccgtctg
     1201 ggaggcgctg gaggagacga aggagaccgg gctgcccgag tggcggcgct ggtcggtggt
     1261 acggatcgtc gaggaccggg acccgtcgag cgcgacgggt gtgacctttc tcgaggtctg
     1321 acgccaccgc ccggatggcg ggccccgctc ctcggggccc gccccctatg tataaggagg
     1381 gagcctcttc tccgcggacg cggggagggg ctcattcctc ccggggcagt cgctgccccg
     1441 ggaggaatgg ccgtcattcc ttcagaaatg gcctccattt ctcgggacat ggccgtcgtt
     1501 tcttcaggaa cggccttcat ttctcgtgaa acggccgcgc tttcccgcga aacgtcggct
     1561 gtttcgcgag aaagagcctt catttctgaa gggatcgccg gggtttcccg ggaaatgacc
     1621 accgtttctg aaggaatggc ggccgtttcc caggaaacgg tcatcgcgct cccatgacat
     1681 ctgtcacccc caactcctga catccctccc tggggcgccg cgctgccggc gcccagaatg
     1741 gcctccatga gatggcaact cacggaggag acgccatgag cacacagaac caggtggtcg
     1801 ccgagctgcg gcgggcgacc cggcgatacg gcaaggtgac cgccctcgac ggggtggacc
     1861 tcgaggtccg ggcgggcgag gtggtcgccc tgctcggacc caacggcgcg ggcaagacca
     1921 ccgccgtcca actgctcctc ggcctgatcc ggcccaccgc cggctccgcc cggctcttcg
     1981 gcctcgaccc ccgcgacacc gccgcccgcg tccgcgtcgg cgccatgctc cagatctcca
     2041 aggtccccga gaccctgaag gtccgcgagc acgtcgagct ggtgtcgagc tactaccccc
     2101 gcccactccc actcggcgag tccctagcgg cggccggcct caccgaggtg ggggaccgcc
     2161 tgttcggcaa gctctccggc ggccagcgcc agcgggtgct cttcgccctc gccctctgcg
     2221 gcaaccccga cgtcctcttc ctcgacgagc ccacggtggg cctcgacgtc gagtcgcggc
     2281 gcgccttctg ggagacggtg cgggcgcggg cgcgcgccgg ctgcaccgtc ttcctcacca
     2341 cccattacct ggaggaggcc gacgccctgg ccagccgcat cgtcctggtg gaccgcgggc
     2401 gcatcgtcgc cgaagggact ccggcggaga tcaaggcgcg ggtgcgcggc cggaagatcc
     2461 gctgccgcac ccgcctcgcc gtggcggagc tggaggccct gcccggcgtg cgcggcgtcc
     2521 ggcgggacgg cgaggttacg gagatgctcg ccgccgaggc cgagcccgtg ctgcgggaga
     2581 tgctggcgcg ggacccgggc ctctccgatc tcgaagtcct gggcgcgggc ctcgaagagg
     2641 ccttcctcgc cctcaccggc aacactcgtt cgaccgacac ggaggtggcg gcatgagcac
     2701 gcagacgatg gcggccgggt cccgcagcct gggccggatc tataccctcg aggcgaagtc
     2761 cgagctgctg aagctcctgc ggctgcccat gtatgcggtg cccaccatcg ccttccccat
     2821 ggtcttctac ctcctcttcg gggtggccat gggggggcgc tcggcgggag gggtgagcta
     2881 tgccacctat ctgatggtct cctacggcgc cttcggggtg gttggcgcca gcctcttcgg
     2941 cttcggggtc ggcctggcgg tggagcgcgg ccaggggtgg atgctgttca agcgggccac
     3001 gccgatgccc cccctggtcc atctggcggg gaggctcacc gtctgcctgc tgttcagcgc
     3061 cgcggtggtc ctgggcctgt tcgcgctggg ggcggcgctc gccggggtca ggctgccggc
     3121 ggcagcctgg ctcaccctcg tcccggtgct cctctcgggc gccctggcct tcggcgcctt
     3181 cgggctcgcc atcggttact gggtggggcc caactcggcg ccggccgtag tcaacgtcat
     3241 ctacctgccc gtggcgttcg cctcggggat gtggttcccc atccaggcga tgccccggtt
     3301 cgtgcaggag ctggcgccgt tcctgccgtc ctatcactac acccagctcg ccttgaaggt
     3361 catcggggcg gacctgggcg gggccgtggc cacgcacgtc gcggccctcg tgggcttcac
     3421 tgtagtgtcg ctcttcctcg cccgggccgg cttccggcgc gacgaggggc gtaccttcgg
     3481 gtaaggtaga gggagaggag acgtcgccgt gagattcctg ccccccaagg atcccgagct
     3541 cggctggacg ccctacgcct ggctgatcta tctcggcttc ttcctgttga agccggtgct
     3601 ggggggagcc tcgcccatag agtggggggc gacgctctcc gccctcggcg tcttccttgg
     3661 cgtctacttc tggggattcc gccagtgggg cccccggatc gtctgggcgg cggcaatgat
     3721 cgccgcgctc ggcttcctct gcgtaccctt caaccccggg gccagcgtct tctggatcta
     3781 cgccggtgcc ttcctggggt actccggccc gccgcgcttc gccctgcgct ggctggcgct
     3841 gctgctggtg ctgctcggcc tccagagctg gtggctggac ctgcggccga gctcctggat
     3901 cacggcggtg gtgttctccg tgatgatctg cggcgtcaac ctccacttct ccgagcggac
     3961 ccgagccaac gcgaagctcc gcgccaccca ggaggaagtg gagcgccttg cccgcacggt
     4021 ggagagggag cgcatcgccc gcgacctcca cgacctcctg gggcatacgc tgtcggtcat
     4081 caccctcaag gccgagctgg ccggcaagct cctcaaccgc gatccggagc gggcggcgga
     4141 ggagatccac gaagtggagc gcatctcccg cgacgccctg cgcgaggtgc gcgaggccgt
     4201 ggtgggttat cgctcggagg ggctcaaggc ggagctggcg cgcgcccgtc tcgccctgga
     4261 gtcgtcgggg atcgccttcg agtacctgct gacgccgctg gacgtcgacc ccgcccggga
     4321 gacggtgctc gccctggcgg tgcgggaggc ggtgaccaac gtcatccgcc acgccggcgc
     4381 gcggagctgc cggatcgcgg tggagcaggg ggcgggcggg gtggtgcggc tggaggtgcg
     4441 ggacgacggc cgcggcggcc tcgccgccga ggg
//
LOCUS       DASPJI010000211         8611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_585431, whole genome shotgun sequence.
ACCESSION   DASPJI010000211 DASPJI010000000
VERSION     DASPJI010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8611)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8611
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585431"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VEL74_05555"
     CDS             <1..889
                     /locus_tag="VEL74_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HYG62024.1"
                     /translation="IVSGIGCSSRFPYYMNTYGMHSIHGRAPAIATGLAVSRPDLSVF
                     VVTGDGDALSIGGNHLMHAIRRNIDLNIVLFNNRIYGLTKGQYSPTSEVGKKTKSTPV
                     GSVDYPFNPLSLALGSGATFVARSVDIFQQHLRETLKRAARHKGTAFVEIYQNCNIFN
                     DKAFTYMTDKETKEDSVLYMEHGKPLVFGKNKDKGIRMRGTEMEVIQLGDGLGPDDCL
                     VWNESLDNPATAFLAAQLLPPEFPTALGVFRSVEMPSYEQRVVEQIQQETDRRGLGKL
                     DDLLRAGDTWEVPSQEAVS"
     gene            906..1298
                     /locus_tag="VEL74_05560"
     CDS             906..1298
                     /locus_tag="VEL74_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62025.1"
                     /translation="MPDLLILAHGGSWDMRFQISSLAASAAAAGERVDIALFFGALDA
                     WAAGRWDDYDPQPPLTSGRLESLDMPPLSEMLASGRESGSIRVYACSASSRLLGLDPG
                     RVQGAVDAILGWQSFSRMIRDAGRVVTL"
     gene            1295..1990
                     /locus_tag="VEL74_05565"
     CDS             1295..1990
                     /locus_tag="VEL74_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62026.1"
                     /translation="MRRILATLLLAACAGTAGAADGKTVAERCAAATASAVVANSSTA
                     EAAPLDSGWTVQAEGTWAAGAADGVLLEIRIDMDRYHSETRTGASGAWRVSQDFNLCG
                     KHTLRVFAMPMVQDGDRLVHCLERGTSAPQTFDLPCEPVARIDSCTWQCTEDDCAGSC
                     TAGARSGVPDYVAHWGLNDSGYHAVEGFSRGPWTHEVVCKPGEKVTFKVRGRNGTGRW
                     SNVAERACGQPGA"
     gene            complement(1987..2355)
                     /locus_tag="VEL74_05570"
     CDS             complement(1987..2355)
                     /locus_tag="VEL74_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62027.1"
                     /translation="MPTRFLDDSTPVVQFDPQLEVSPFALFRRMKEGKPPLLVDVRPA
                     PGPLSLRGAIPYPGPDWSPPAGEDVVLFDGDGATAVDGARHMQAAGYPRVRALFGGLD
                     LYEFSLDPEVVGEETFLVKC"
     gene            complement(2388..3614)
                     /locus_tag="VEL74_05575"
     CDS             complement(2388..3614)
                     /locus_tag="VEL74_05575"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HYG62028.1"
                     /translation="MRIFAFEGLRYTPTAGETGELAALPYDQIDNSLRDRYHAQSPYQ
                     FVHLTRPVPPEGEDPYRHSAGLHGRWLAEGAVARDPRPALYPYAVELTEGDRRLGLMA
                     LVGYEDASHIRPHEQTLDKPLAERLALLEATRVDYEPIFLLSEDGGRLEELLQEDMAA
                     QPIVRHRDPAGHIHLLYKVDDPARIARYQEALQVPAAIADGHHRYKVGQRFAREHGAK
                     EGTAAAAKLAVITSIQSPALTIRPTHRGLRETADLSKLAGVAVTAEPFRGSTGDEMLE
                     AVAAALQPAVGVWIAGHEPEVWHLDPTMVPNATSPGVPQLPVEVLHKTILPALGYPPE
                     AATDGTVVYRDNADQLFTEVKEGTFGTAFWLPPTAPAEFSLAIAGGNMLPPKSTRFMP
                     KVMSGLVWADHDSKLL"
     gene            3684..4163
                     /locus_tag="VEL74_05580"
     CDS             3684..4163
                     /locus_tag="VEL74_05580"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYG62029.1"
                     /translation="MQSLDDIRRLFAYDAWANRETLASLRSASSRSAPPEQALRWMAH
                     IAATEGLWLSRILADGERVVVWPELTLGECEERTAAMARRWTVYLDGITSGELPRAVT
                     YVNSQGEPWTSTVADILTHTVMHSVHHRAQIAAELRRHGHTPAYTDFIHAVRRGFVE"
     gene            complement(4167..4898)
                     /gene="mtgA"
                     /locus_tag="VEL74_05585"
     CDS             complement(4167..4898)
                     /gene="mtgA"
                     /locus_tag="VEL74_05585"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HYG62030.1"
                     /translation="MIAPPYTVPRRGQTRLLLLSGLAAVILWLGWQVLTWPDVAALEE
                     ERPETTAFIERYRGRGWFGNRREVEWKWVPYRRISSNLKRAVIVSEDIEFFSHDGFSR
                     RETRAALEDAWEEKKLPRGASTITQQLAKNLWLSPSRNPVRKVKEAILTRQLEEHLSK
                     RRILEIYLNVVELGPGIYGAEAATRHYFGKSAASLTTRQAAELAAVLPRPKSWHPGSK
                     SKSYQSKVRSIQRRMAKASWLRRSL"
     gene            5046..6854
                     /locus_tag="VEL74_05590"
     CDS             5046..6854
                     /locus_tag="VEL74_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYG62031.1"
                     /translation="MNRAAYVRRGPWSNTLRITGVALVATLGLAASGRAASPQTPDDD
                     EKARRPRVQQTETFGLADPRGAAFPDKNQPAPPSEKVGVALDAVSQVALAPLDVDALL
                     AEDQNKARVTMTKVLRYGVGRDLDVRAQDGNWFDLPGGGRLWALDVVSTGALGLRLQF
                     RNVDLPAGGSLAVYNPDASEDKALGAFGAFDRDLETYSAGTPDRPSDFWSGTIAGERA
                     RVEYYAPSGAGDELPFALTRLQHIYVDPVQAVSGNKAAGSCHNDVACHPDWADLANAV
                     AGIGVVGHDALFCTGQLINNLSQDFTPYFLTANHCLSSPFEAQSTEIFWFYQKESCGG
                     SVPSLSSSPTSRGASLVATDDPSDFTLLLLEGAVPNGVFWAGWTAQGIEDGVGATAIH
                     HPSGDHKRISFGNTGGQPVCGIALDDHVKISWTGNAVTEPGSSGSGIFRNDTGQLFGQ
                     LHCGPSSCGASANNLNDSYGSFSTTFAKIRNPLNGGSDDNSEPNDTCGKPRVARKNQN
                     LTNRIVKWADRDFYRIRVPKNKTLTATVTFNHGNGDIDTRLYRACNQEAVAVTEGEGD
                     TEVLTYRNTGKPANFVLEVFLYSDTRNSYNVKFAIK"
     gene            6933..7916
                     /locus_tag="VEL74_05595"
     CDS             6933..7916
                     /locus_tag="VEL74_05595"
                     /inference="COORDINATES: protein motif:HMM:NF037020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HYG62032.1"
                     /translation="MPPITVPLRAPGLAPGVWVQGPQVSLKSGAVTLVDFWESTCVNC
                     LRTLPYLRAWHERYSPRGLVIVGVHTPEFEVTADPAVVAAAVAAEAIPYPVLLDAGRA
                     TWQLFANKYWPAKYLIDARGYLRYEHAGEGAYGETEEWIQRLLREAGDTAPMPALLEP
                     VREVDRPGAVCQPATREAHLGWHRGRLLAAEGYRPGEEVRHAAREEPAPPGLFTARGL
                     WLHEAEYLEAREPGAGLELVCDAAEVNLVLAPPVGETAELEIEAGGAPVAVEERGADL
                     VARDGRTFAVWDRPRMVRLVDSPDFRRRHLRLRFLRPGARAYAFSFGTVCT"
     gene            8102..>8611
                     /locus_tag="VEL74_05600"
     CDS             8102..>8611
                     /locus_tag="VEL74_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG62033.1"
                     /translation="MKLEHLTDEELIQEARRRSGPAIQECLDVLFRRHYPRVAYWCLR
                     ICGSREAAADLAQEVFLRVQSRLDSFRMESRFSTWLYQVTRSVAINHGEAARKRSAPL
                     DGLDSAPELADPVPAADEILVTGQSVAELKEILEQDLEPLEAKVLYLHYGDGLTLPGI
                     TAMLGLQNKS"
BASE COUNT         1304 a         3034 c         2996 g         1277 t
ORIGIN      
        1 catcgtctcg ggcatcggct gctcctcgcg cttcccgtac tacatgaaca cctacgggat
       61 gcactcgatc cacggccgcg ccccggccat cgccaccggc ctggcggtct cccgcccgga
      121 cctgtcggtg ttcgtggtga ccggggacgg ggacgcgctg tcgatcgggg gcaatcacct
      181 gatgcacgcc atccggcgaa acatcgatct gaatatcgtg ctgttcaaca accgcatcta
      241 cggcctgacc aaggggcaat actcgccgac cagcgaggtc ggcaagaaga ccaagtcgac
      301 gccggtgggg tcggtggact atccgttcaa cccgctgtcg ctggccctgg gctccggcgc
      361 gaccttcgtg gcgcggagcg tcgacatctt ccagcagcac ctgcgcgaga cgctgaagcg
      421 tgccgcgcgg cacaagggga ccgccttcgt cgagatctac cagaactgca acatcttcaa
      481 cgacaaggcg ttcacctaca tgacggacaa ggagaccaaa gaggacagcg tcctctacat
      541 ggagcacggc aagcccctgg tcttcggcaa gaacaaggac aaggggatcc ggatgcgggg
      601 taccgagatg gaggtgatcc agctcggcga cggcctgggt ccggacgact gcctggtctg
      661 gaacgagtcg ctggacaacc cggcgacggc gttcctggcg gcgcagcttc tgccgccgga
      721 gttcccgacc gccctgggtg tcttccgcag cgtcgagatg ccgagctacg agcagcgggt
      781 agtggagcag atccagcagg agaccgaccg ccgcggcctc ggcaagctcg acgacctcct
      841 ccgcgccggc gacacctggg aggtccccag ccaggaagcg gtgagctgag gggcgtggag
      901 ccttcgtgcc ggacctgctg atcctcgccc atggcggctc gtgggacatg cgctttcaga
      961 tctcgtcgct cgccgccagc gccgcggcgg cgggggagcg ggtcgacatc gccctgttct
     1021 tcggcgccct tgatgcctgg gcggctgggc gctgggacga ctacgacccc cagccgccgc
     1081 tgacgtccgg gcggctcgag tcgctggaca tgccaccgct ctccgagatg ctggcgtccg
     1141 gccgggagag cggctccatc cgtgtctacg cgtgcagcgc gtcgtcgcgc ctgctcggcc
     1201 tcgacccggg ccgggtgcag ggcgcggtcg acgccatcct cgggtggcag agcttctcaa
     1261 ggatgatccg cgacgccgga cgggtggtga ccctgtgagg cggatcctcg ccactcttct
     1321 tctcgcggcc tgtgcgggga ccgccggcgc cgctgacggc aagaccgtgg cggagcggtg
     1381 cgccgcggcc acggcgtcgg cggtcgtcgc caactcgtcc acagcggagg ccgcgccgct
     1441 ggactccggc tggacggtcc aggccgaggg gacctgggcg gcgggtgccg ccgacggcgt
     1501 gctcctcgag atccgcatcg acatggaccg ctatcactcc gagacgcgca ccggcgcctc
     1561 gggcgcgtgg cgcgtctccc aggacttcaa cctctgcggc aagcacacgc tccgcgtctt
     1621 cgccatgccc atggtccagg acggggaccg cctggtccac tgcctggagc ggggcacctc
     1681 ggctccgcag acgttcgatc tcccctgcga gccggtggcg cggatcgact cctgcacctg
     1741 gcagtgcacc gaagacgact gcgcgggaag ctgcaccgcc ggcgcccgct ccggggtgcc
     1801 ggactacgtc gcccactggg gcctcaacga cagcggctat cacgcggtgg aggggttctc
     1861 gcgcggcccg tggacccacg aagtggtttg caagccgggc gaaaaggtca ccttcaaggt
     1921 gcggggaagg aacggcaccg gccgctggtc caacgtggcc gagcgggcct gcggccagcc
     1981 cggcgcctag cacttcacca ggaacgtctc ctcgcccacc acctcgggat cgagggagaa
     2041 ctcgtagaga tccaggccgc cgaagagggc gcggacgcgg gggtagccgg cggcctgcat
     2101 gtggcgggcg ccgtccaccg cggtggcgcc gtcgccgtcg aacagcacca cgtcctcgcc
     2161 ggcgggcggc gaccagtcgg ggccgggata ggggatcgcg ccgcggagcg acagggggcc
     2221 cggcgcaggc ctcacgtcga ccagcagcgg cggcttccct tctttcatcc gccggaacag
     2281 tgcgaaaggg gagacctcga gctgcgggtc gaactggacc accggggtcg agtcgtcgag
     2341 gaagcgggta ggcatgacgg ctcgaccccc taagaacccg gcaccggcta cagcagcttc
     2401 gagtcgtggt ccgcccagac caggccggac atgaccttgg gcatgaagcg ggtcgacttc
     2461 ggcggcagca tgttgccgcc ggcgatggcg agggagaact cggccggcgc ggtaggcggc
     2521 agccagaagg cggtgccgaa cgtcccttcc ttcacctcgg tgaagagctg gtcggcgttg
     2581 tcacggtaga ccacggtgcc gtcggtggcc gcttcgggcg ggtagccgag cgcgggaagg
     2641 atcgtcttgt ggaggacctc caccgggagc tgcggcacgc ccggggaggt ggcgttcggc
     2701 accatggtgg ggtcgaggtg ccagacctcc ggctcgtggc cggcgatcca gacccccacc
     2761 gccggctgca gggcggcggc cacggcctcc agcatctcgt cgcccgtgga gccgcggaac
     2821 ggctccgccg tgacggcgac gcccgcgagc ttggagaggt cggccgtctc gcgcagaccc
     2881 cggtgggtgg ggcggatggt cagcgccggc gactggatgc tggtgatcac cgccagcttg
     2941 gccgccgccg ccgtcccctc cttggcgcca tgctcgcggg cgaagcgctg gcccaccttg
     3001 tagcggtgat ggccgtcggc gatggctgcg ggtacctgca gcgcctcctg gtagcgggcg
     3061 atgcgggcgg ggtcgtccac cttgtagaga agatggatgt ggcccgccgg gtcgcggtgg
     3121 cggacgatcg gctgggccgc catgtcctcc tggagcagct cctcgagccg gccgccgtcc
     3181 tccgagagga ggaagatggg ctcgtagtcg acccgggtgg cctcgagcag cgccaggcgc
     3241 tcggccagcg gcttgtccag cgtctgctcg tgcggccgga tgtgggaggc gtcctcgtac
     3301 cccaccagcg ccatgagccc gagccgcctg tccccctccg tcagctcgac ggcgtacggg
     3361 tagagcgcgg ggcgcggatc ccgcgccacg gccccctcgg cgagccagcg gccatgcagg
     3421 ccagcggagt gccggtacgg gtcctctccc tccggcggca ccggacgggt caggtggacg
     3481 aactgataag gcgactgcgc gtggtagcgg tcgcgcaggc tgttgtcgat ctggtcatag
     3541 ggaagggcag ccagctcgcc ggtctccccg gcggttggcg tgtatcgcag tccctcgaaa
     3601 gcaaagatac gcataggatg tctcctgggc agagcccctc ggaggggcgc cgtattctat
     3661 cgtggagagc agggaggaac ccaatgcaga gcctggacga catccggcgc ctcttcgcct
     3721 acgatgcgtg ggccaaccgc gagaccctcg cctccttgag gtcggcctcg tcgaggtcgg
     3781 ccccgccgga gcaggcgctg cgctggatgg ctcacatcgc cgccaccgag gggctctggc
     3841 tgagccgcat cctggcggac ggcgagcgcg tcgtcgtctg gcccgagctg accctcggcg
     3901 agtgcgagga gcggacggcg gcgatggccc ggcgctggac ggtctatctg gacggcatca
     3961 cctccgggga gctgccgcgg gccgtcacct atgtcaactc gcagggggag ccctggacga
     4021 gcacggtggc ggacatcctg acgcacacgg tgatgcactc ggttcaccat cgcgcccaga
     4081 tcgcggccga gctgcggcgg cacgggcata cccccgccta caccgacttc atccacgccg
     4141 tgcggcgcgg cttcgtcgag tgagcctcac aagctgcgcc gcagccagga agccttggcc
     4201 atccggcgct ggatcgagcg gaccttgctc tggtaggact tgctcttgct ccccgggtgc
     4261 cagctcttgg ggcgcgggag gacggcggcc agctcggcag cctgccgggt ggtgaggctc
     4321 gcggccgact tgccgaaata gtggcgcgtc gcggcctcgg cgccatagat gccgggcccg
     4381 agctccacca cgttcaggta gatctccagg atccgccgct tgctcagatg ctcctcgagc
     4441 tggcgggtga ggatcgcctc cttcaccttg cggaccgggt tgcgcgacgg cgagagccat
     4501 aggttcttgg cgagctgctg ggtgatggtg gaggcgccgc gggggagctt cttctcttcc
     4561 caggcgtcct ccagggccgc ccgggtctcg cgccgggaga aaccgtcgtg ggagaagaac
     4621 tcgatgtcct cgctgacgat caccgcccgc ttgaggttcg acgagatccg ccggtacggc
     4681 acccacttcc actccacctc gcgccggttg ccgaaccacc cccggccgcg gtagcgctcg
     4741 atgaacgccg tggtctccgg cctctcctct tccagagccg ccacgtccgg ccaggtgagc
     4801 acctgccagc ccagccagag gatgacggcg gccaggccgg acaggaggag aaggcgcgtc
     4861 tgtcctctgc gaggcaccgt ataaggtgga gcgatcatgg ggagactgaa tcatatggca
     4921 agaggcgcgg gaagagggtc ccccaccgtg gtgtatactg ttagaccata ttcggagaag
     4981 tccatggatc acggcagcac ctatccaacc gatcccggga cgcccatccg ccagaaggag
     5041 gaagtatgaa cagagcggct tacgtacgtc gcggcccgtg gagcaacacc ctccgcatca
     5101 cgggggtcgc gctcgtggcg accctgggcc tcgcggcctc cggccgcgcg gcatcccccc
     5161 agacgccgga cgatgacgag aaggcgcgcc gcccccgcgt ccaacagacc gagaccttcg
     5221 gcctcgctga tccccggggc gccgccttcc ccgacaagaa ccagccggct cccccctccg
     5281 agaaggtcgg cgtggcgctg gacgccgtct cccaggtggc gctcgcgccg ctggacgtcg
     5341 acgcgctcct ggcggaggac cagaacaagg cccgggtcac gatgaccaag gtgctgcgct
     5401 acggcgtcgg ccgtgacctc gacgtgcgcg cccaggacgg caactggttc gacctgccgg
     5461 gcggcggccg cctgtgggcc ctcgacgtgg tgtcgaccgg ggccctcggc ctccgtctcc
     5521 agttccggaa cgtcgacctg cccgcggggg gcagcctggc ggtctacaac cccgacgcgt
     5581 cggaggacaa ggccctgggc gcgttcggcg ccttcgaccg cgacctcgag acctactccg
     5641 ccgggacgcc cgaccggccg tccgacttct ggtccggcac catcgccggc gagcgcgccc
     5701 gcgtcgagta ctacgcgccc agcggcgccg gggacgagct gccgtttgct ctgacccgcc
     5761 tgcagcacat ttacgtcgac ccggtgcagg cggtgtccgg caacaaggcc gccggaagct
     5821 gccacaacga cgtcgcctgc catccggatt gggccgacct cgccaacgct gtggccggca
     5881 tcggcgtcgt cggccacgac gcgctgttct gcaccggtca gctcatcaac aacctgtcgc
     5941 aggacttcac cccgtacttc ctgaccgcga accactgcct gagcagcccg ttcgaggcgc
     6001 agtcgacgga gatcttctgg ttctaccaga aggagtcctg cggcggctcc gtgccgtcgc
     6061 tctcctcgtc cccgacgtcg cgcggcgcga gcctcgtggc gaccgacgat ccgtccgact
     6121 tcacgctgct gctgctcgag ggcgcggtgc ccaacggtgt cttctgggcc ggctggacgg
     6181 cgcagggcat cgaggacgga gtcggcgcca ccgccatcca tcacccgtcg ggcgaccaca
     6241 agcggatcag cttcggcaac acgggtggcc agccggtctg cggcattgcc ctcgatgacc
     6301 acgtgaagat cagctggacg ggcaacgcgg tcaccgagcc gggctcctcg ggctcgggca
     6361 tcttccgcaa cgacacgggc cagctcttcg gccagctcca ctgcggcccc tcctcctgcg
     6421 gcgcctcggc caataacctg aacgactcct acgggtcctt ctcgaccacc ttcgccaaga
     6481 tccgcaaccc cttgaacggt ggcagcgacg acaactcgga gcccaacgac acctgcggca
     6541 agccgcgcgt cgcgcgcaag aaccagaacc tcaccaaccg catcgtcaag tgggccgacc
     6601 gtgacttcta ccggatccgg gtcccgaaga acaagaccct caccgccacc gtcaccttca
     6661 atcacggcaa cggtgacatc gacacccgcc tgtaccgcgc ctgcaaccag gaggcggtgg
     6721 ccgtgaccga aggggagggc gacaccgagg tcctgacgta caggaacacg ggaaagccgg
     6781 ccaacttcgt gctcgaggtc ttcctctact cggacacgcg gaacagctac aacgtgaagt
     6841 tcgcgatcaa gtaagctgac cgcgaccgcg cgctccaacc acggaggggg tggcgacgcc
     6901 ccctccgttt cgcttttctg gagggacctg acgtgccgcc gatcaccgtg ccgctccgcg
     6961 cccccgggct cgctccgggc gtctgggtcc aggggccgca ggtctcgctc aagagcggcg
     7021 cggtcaccct ggtggacttc tgggagtcca cctgcgtcaa ctgcctgcgc accctgccgt
     7081 acctgcgggc ttggcacgag cgctactcgc ctcgcggcct ggtcatcgtg ggcgtccaca
     7141 cgccggagtt cgaggtcacc gccgaccccg ccgtggtggc cgcggcggtg gcggccgagg
     7201 cgatccccta tcccgtcctg ctggacgccg gccgcgccac ctggcagctc ttcgccaaca
     7261 agtactggcc ggccaagtac ctgatcgacg cccgcggcta tctgcgctac gagcacgccg
     7321 gcgaaggggc ctacggcgag accgaggagt ggatccagcg cctgctgcgc gaggccgggg
     7381 acacggcgcc catgccggcg ctgctcgagc cggtgcggga ggtggaccgg cccggcgccg
     7441 tctgccagcc cgccacccgt gaggcccatc tgggctggca ccgggggagg ctcctggccg
     7501 ccgagggcta ccggccgggg gaggaggtcc ggcacgccgc ccgggaggag ccggcgcctc
     7561 ccggcctgtt caccgcccgc ggcctgtggc tgcacgaggc ggagtacctg gaagcccgcg
     7621 agccgggggc cgggctggag ctggtctgcg acgcggccga ggtgaacctg gtgctggcgc
     7681 cgccggtggg ggagacggcg gagctggaga tcgaggccgg cggcgcgccc gtcgcggtgg
     7741 aggagagggg agcggacctg gtggcgaggg acgggcggac gttcgccgtc tgggaccgtc
     7801 cgcgcatggt gcggctcgtc gactccccgg atttccggcg gcgccatctg cgcctgcgct
     7861 tcctcaggcc gggcgcccgg gcctacgcgt tcagcttcgg gacggtctgc acctgaccag
     7921 cctgttccat ctcccctttg cccctgtctc actctgcatg accgtgtcgt ccgttgtttt
     7981 cagggattct caaccatacg ccccggctgt ccgttccggc ggctcatcct gtaggctagg
     8041 gtcagtacgc ccggcggtgg ggaggcagcc ggcctcccgc cacattcgga gaccgagggc
     8101 cttgaaattg gagcacctga ccgatgagga gctgatccag gaggcgcggc gccgctccgg
     8161 ccccgccatc caggaatgcc tcgacgtcct gttccgacgg cactacccgc gggtcgccta
     8221 ctggtgcctg cggatctgcg gcagccgcga ggccgccgcc gatctcgccc aggaggtctt
     8281 cctccgcgtc caatcccgcc tcgactcctt ccgcatggag agccgcttct ccacctggct
     8341 ctaccaggtc acgcgcagcg tcgccatcaa ccacggcgag gcggcgcgca agcggtcggc
     8401 gccgctggac ggcctcgact cggctcccga gctcgcggac ccggtgcccg ccgccgatga
     8461 gatcctggtg accggccagt cggtggcgga gctcaaggag atcctggagc aggacctgga
     8521 gccgctcgag gccaaggtcc tctacctcca ctatggagac gggctcaccc tgcccggcat
     8581 cacggcgatg ctgggcctcc agaacaaatc g
//
LOCUS       DASPJI010000212         5968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_816821, whole genome shotgun sequence.
ACCESSION   DASPJI010000212 DASPJI010000000
VERSION     DASPJI010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816821"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VEL74_05605"
     CDS             complement(<1..830)
                     /locus_tag="VEL74_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62034.1"
                     /translation="MWTPSDAKKLFRALAVLALLGLPVLDGAVPAQTSPSATSTSPES
                     RQELRQLLERRYEVLPIRNGVLLRPRQERLGVRTIELSGDSVAINGERVSNGVLRAWL
                     GEDADPILRLQAMEPAERAALFNMDQAGGAEPVPAGEDEEAGEIPEPAMDTAAGIPPV
                     PEPPAAPPPPVVAEAGEDGEDGEEPATRLGSQVNVGGPITVSRGEIAEEAVAVLGPVR
                     VDGEVVQDAVAVLGAVRINGRVGGEVVAVMGSVTLGPDAEVMGDVTSVGGNVRRAPGS
                     R"
     gene            complement(814..1383)
                     /locus_tag="VEL74_05610"
     CDS             complement(814..1383)
                     /locus_tag="VEL74_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62035.1"
                     /translation="MTMHPSHDPLTRWLAAERDGLTDEAEELLLELFEADALPRLAPP
                     AGFADRVLARVAVEAAVERAAVPVQPGFSLFRRGWLRWVIALCLLATGLSVAWLPQTV
                     AVFAGLWSPAGFLQSSIRAVLDAIRWMASLADVGQTLLAVAEAVALSLARPQVMVIAL
                     VCLVVSVTSFRFLRGLVSRDRSWAYVDPI"
     gene            complement(1396..2043)
                     /locus_tag="VEL74_05615"
     CDS             complement(1396..2043)
                     /locus_tag="VEL74_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG62036.1"
                     /translation="MLSDAELVAQARGGSQDAFRELVLRFERPVFSLVARMVHDPATA
                     EDLAQEVFVKAFRRLDTYDPQRKLSSWLFKIAHNTTIDFLRRGELDTVPLEAREDEGD
                     RGSLAGVLADETAEGPGAAAERRQMARSLERAIARLRPEYRESVLLFYVEGLAYQEIC
                     EITGQPLGTVKTNLHRARKELAAVMTEMGWSPAPNIGTGSGSGAKPVPPPSRRAG"
     gene            2210..2779
                     /locus_tag="VEL74_05620"
     CDS             2210..2779
                     /locus_tag="VEL74_05620"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HYG62037.1"
                     /translation="MRTLQTALLTLLLAVPAAMPAAAGTLSGVTLPDKVDAGGQSLAL
                     NGMGLRKKLVIKVYVGGLYLPQKAKADGRQVLAADRPYRMAMHFLYDVSKEQMCDAWS
                     EGLEANTPNASAQVKKDFQTLCGWMDGVGKGQKLNLTYTPGEGTTVEISGKAKGTLPG
                     KATADAILATWIGPEPGPGDDFKKAVLGG"
     gene            complement(3281..4102)
                     /locus_tag="VEL74_05625"
     CDS             complement(3281..4102)
                     /locus_tag="VEL74_05625"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020413446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HYG62038.1"
                     /translation="MTPFQAAVLAIVQGVTEFLPISSSGHLILIPYFLRWEDQGLSFD
                     IATHTGTLLAIIAYFHRDVRDLILGFFDGRPLSQSGDFAPRPMAWLIGLATIPAGLAG
                     LLFKDWIAGEARNPLLIAGTAIGYGLLLGLADRAGRRDREIGTIDWKAALIIGAAQAL
                     ALVPGTSRSGITITAALLLGFTRPAAARFSFLMIIPISVLVAVMDAVDLARAGVTAAE
                     LVPMGVGLVVSAVVGYMVIAWLLAWLRRQSLTVFVVYRILLGLVILATVWFRGGL"
     gene            4266..5360
                     /gene="rfbB"
                     /locus_tag="VEL74_05630"
     CDS             4266..5360
                     /gene="rfbB"
                     /locus_tag="VEL74_05630"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HYG62039.1"
                     /translation="METLLVTGGAGFIGCNFVRLALAEPPEVRLVVLDKLTYAGSLEN
                     LADVLGHPRFTFVQGDIADRAEVRKAFAEHRPTAVVNFAAESHVDRSIEDASAFIQTN
                     IVGAFEMLEGARRYIEGQDGAARERFRFLHVSTDEVYGSLGPTGLFSETTAYAPNSPY
                     AASKAGADHLVRAYHETYGLPALITNCSNNYGPYQFPEKLIPLMLLNALEGKALPIYG
                     DGANVRDWIYVEDHCRGVLAALRHGRPGEKYNLGGRSERTNLQVVDTICDILERERPA
                     AENPALAARGIASYRDLKTFVADRPGHDRRYAIDDEKARTELGWETRHDFASGLAATA
                     CWYLANGAWCQAVQSGRYDRERLGLLEGKR"
     gene            5357..5905
                     /gene="rfbC"
                     /locus_tag="VEL74_05635"
     CDS             5357..5905
                     /gene="rfbC"
                     /locus_tag="VEL74_05635"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HYG62040.1"
                     /translation="MKFTPTRIPEVILVEPDVFRDARGYFFETHHQKKYAEGGIPELF
                     VQDNQSFSVARTLRGLHAQLRRPQGKLVRAIQGEIFDVGVDIRPGSPTFGQWVGEVLS
                     GDNFRQLYIPPGFAHGFCVLSETAVVHYKCTDLYDKDDEIGLVWNDPEIGIEWPLADP
                     LVNDKDGNAPRLAEIVHKLTLP"
BASE COUNT          990 a         2159 c         2029 g          790 t
ORIGIN      
        1 cgcgaccccg gcgcccgccg cacgttgccg ccgacgctgg tgacgtcacc catcacctcg
       61 gcgtccggcc ccagggtcac gctgcccatc accgccacca cctcgccgcc gaccctgccg
      121 ttgatgcgga cggcgccgag caccgcgacg gcgtcctgca ccacctcgcc gtcgacgcgg
      181 accggcccga ggaccgccac ggcctcctcg gcgatctccc cccggctgac cgtgatcggg
      241 ccgccgacgt tgacctggct gcccagacgg gtcgccggct cctcgccgtc ctcgccgtct
      301 tccccggcct ccgccaccac cggcggcggt ggcgccgcgg gcggctccgg caccgggggg
      361 attccggcgg ccgtgtccat cgccggctcg gggatctccc cggcctcttc gtcctcgccg
      421 gccggcacgg gctccgcgcc gccggcctga tccatgttga agagggcggc gcgctcggcc
      481 ggctccatgg cctgcaggcg caggatgggg tcggcgtcct ccccgagcca ggcgcggagc
      541 acgccattgc tcacccgctc gccgttgatc gcgacgctgt cgccgctcag ctcgatggta
      601 cggacgccga gccgctcctg ccgcggccgc agcagcacgc cgttgcggat cggcaggacc
      661 tcgtagcgcc gctcgaggag ctggcgcagc tcctgccggc tctcgggaga cgtggaggtg
      721 gcggacggag acgtctgcgc cggcacggcg ccatccagaa cgggcaggcc gagcagcgcg
      781 agcacggcca gggcccggaa gagtttcttg gcatcagatg gggtccacat aggcccaact
      841 cctatcgcgc gagaccagcc cgcgcaggaa acggaacgac gtgacggaga ccaccaggca
      901 gaccagggcg atgaccatga cctggggccg cgccagcgac agggcgacgg cttcggccac
      961 ggcgagcagg gtctgcccca cgtccgccag cgaggccatc caacggatgg cgtcgaggac
     1021 ggcgcggatg gaggactgga ggaagccggc cgggctccag aggccggcga agaccgcgac
     1081 ggtctgcggc agccaggcga cggagaggcc ggtggcgagg aggcagagag cgatgaccca
     1141 gcgcagccag ccccttctga agaggctgaa gccgggctgg acgggtacgg cagccctctc
     1201 cacggccgct tccaccgcca cacgggcgag cacgcggtcc gcgaagcccg cgggcggcgc
     1261 gagccggggg agcgcgtctg cctcgaacag ctcgagcaga agctcctcag cctcgtccgt
     1321 gaggccgtcc cgctcggcgg cgagccagcg ggtcagcgga tcgtgggaag gatgcatcgt
     1381 catcggtctc ctctctcaac ccgccctacg agacggaggc ggaacgggtt tcgcgccgga
     1441 cccggagccc gtcccgatgt tcggggccgg cgaccagccc atctccgtca tcaccgcggc
     1501 cagctccttg cgcgcccggt ggaggttggt cttgaccgtt cccaggggct ggccggtgat
     1561 ctcgcagatc tcctggtacg ccagcccctc gacatagaac agcagcaccg actcgcggta
     1621 ctcggggcgg aggcgggcga tggcccgctc gagggagcgc gccatctgcc ggcgctcggc
     1681 cgccgccccc ggtccctcgg cggtctcgtc ggcgaggacg ccggccaggg agccgcggtc
     1741 cccctcgtcc tcccgggcct ccagggggac ggtgtcgagc tcaccccggc gcaggaagtc
     1801 gatggtcgtg ttgtgggcga tcttgaacag ccagctcgag agcttgcgct gcgggtcgta
     1861 ggtgtcgagc cggcggaacg ccttgacgaa gacctcctgg gccaggtcct ccgcggtggc
     1921 cggatcgtgc accatccgcg ccaccaggga gaagaccggg cgttcgaagc gcagcaccag
     1981 ctcgcggaac gcgtcctgcg agccgccacg ggcctgggcc accagctcgg cgtcgctcag
     2041 catcggtcgc tcagcatcaa gggagggata cgtcccgcag ccaccggtgt tgcgcacggt
     2101 ctctccgcgc gcgctctctc gacgtcgacc ggggatcggc tacacttcct gttcgaggtt
     2161 cccgatctta acaaatccct cgcgcaacac gagacgacgg aggacgacga tgcgaactct
     2221 ccaaaccgcc ctgctcaccc tgctgctcgc cgtcccggcc gccatgccgg ccgccgccgg
     2281 cacgctgtcg ggcgtcaccc tccccgacaa ggtcgacgcc ggcgggcaga gcctggccct
     2341 gaacggcatg ggcctgcgca agaagctggt gatcaaggtc tacgtcggcg ggctctacct
     2401 gccgcagaag gccaaggccg acggccggca ggtcctggcc gccgaccgcc cctaccgcat
     2461 ggccatgcac ttcctctatg acgtcagcaa ggagcagatg tgcgacgcct ggtcggaggg
     2521 gctcgaggcc aacaccccca acgcctcggc gcaggtgaag aaggacttcc agaccctgtg
     2581 cggctggatg gacggcgtcg gcaaggggca gaagctcaac ctgacctaca cccccggcga
     2641 gggcaccacg gtggagatct ccggcaaggc caaggggacg ctccccggca aggccacggc
     2701 cgatgccatc ctcgccacct ggatcggccc cgagcccggc ccgggcgacg acttcaagaa
     2761 ggccgtcctc ggcggctagg cccgcgagac cgcctgcccc ctcccggagg gggcaagccc
     2821 caccccccgc cgccggtccg gaaggatttt cgtcgcagag cagggttccc gagggtcgcg
     2881 acccgggaag aacgtcgcag aggcgcgttc attcgtcgca gatgagcccc gctctgcgac
     2941 gaatgaacgc agctctgcga cgaacgaact ctggtctgcg acgaatggag ccagctctgc
     3001 gatgtttgag cgcagctcta cgatgtttgg gcgcagctct gcgacgtttc agcgcagctc
     3061 tgcgacgatc ggatcgaaag agggaggtgg ctcggaagcc gcgacgaagg gggcggtggt
     3121 gcctccccta catttcaggc ccctgaagta aaaaggagga gcgcgatccg ggcgctcctc
     3181 ttgggaggga tgagaggaag ggtcttgacc tcccccgcaa cccggagctt agccaagaca
     3241 tcgatgcttg tcaatatgag cttcgactgt cacgagagtg tcacagcccg ccgcggaacc
     3301 agaccgtggc caggatcacc agtcccagca ggatccggta gaccacgaac accgtgaggc
     3361 tctgccggcg cagccaggcg agcagccagg cgatcaccat gtagcccacc acggccgaca
     3421 ccaccagccc gacccccatg ggcaccagct cggcggccgt cacccccgcc cgcgccaggt
     3481 ccaccgcgtc catgaccgcc accaggacgc tgatcgggat gatcatcagg aaggagaagc
     3541 gggcggcggc cgggcgggtg aagccgagga gcagcgccgc ggtgatggtg atccccgagc
     3601 gcgaggtgcc cggcaccagc gccagcgcct gcgcggcgcc gatgatcagc gccgccttcc
     3661 agtcgatggt gccgatctcg cggtcgcgcc ggccggcgcg gtcggccagg ccgaggagca
     3721 ggccgtagcc gatggcggtg ccggcgatga gcagcgggtt gcgcgcctcg ccggcgatcc
     3781 aatccttgaa cagcagcccg gcgaggccgg ccgggatggt ggccaggccg atcagccagg
     3841 ccatggggcg cggcgcgaag tcccccgact gcgacagcgg ccggccgtcg aagaagccga
     3901 ggatcaggtc gcggacgtcg cggtggaagt aggcgatgat ggcgaggagc gtcccagtgt
     3961 gggtggcgat gtcgaaagac agcccctggt cctcccacct caggaaatag gggatcagga
     4021 tcaggtgccc cgacgagctg atcggcagga actcggtcac cccctggacg atcgccagca
     4081 ccgcggcctg gaagggcgtc accggcgggc tctccgcgaa gtctcggaca tgtggtgctc
     4141 ctctcctctc gccaggtgcc ccccgagggg cgcccgattg tatgccagga ggaggccatg
     4201 aaagcggcta tactctcgcc ggctccgcgg agccttcgac cgaacactcg aacggaatca
     4261 cgaacatgga aacgctcctc gtcaccggcg gcgccggttt catcggctgc aacttcgtgc
     4321 ggctcgccct cgcggagccg cccgaggtcc ggctggtggt gctcgacaag ctcacctacg
     4381 cgggcagcct cgagaacctg gccgacgtcc tcggccaccc ccgcttcacc ttcgtccagg
     4441 gcgacatcgc cgaccgggcc gaggtgcgca aggccttcgc cgagcaccgg cccacggcgg
     4501 tggtcaactt cgccgccgag agccacgtcg accgctcgat cgaggacgcc agcgccttca
     4561 tccagaccaa catcgtcggc gccttcgaga tgctggaagg ggcgcggcgc tatatagaag
     4621 gacaggacgg cgccgcccgc gagcgcttcc ggttcctcca cgtctcgacc gacgaggtct
     4681 acggcagcct cggcccgacc ggcctgttca gcgagaccac ggcctacgcg cccaactcgc
     4741 cgtacgccgc ctccaaggcc ggcgccgacc acctggtgcg cgcctatcac gagacgtacg
     4801 ggctgccggc gctgatcacc aactgctcca acaactacgg cccctaccag ttccccgaaa
     4861 agctgatccc cctgatgctg ctgaacgccc tggaggggaa ggcgctgccg atctacggcg
     4921 acggcgccaa cgtccgcgac tggatctacg tcgaggacca ctgccggggg gtgctggcgg
     4981 cgctgcgcca cgggcggccc ggggagaagt acaacctggg cggccgaagc gagcgcacca
     5041 acctgcaggt ggtggacacc atctgcgaca tcctggagcg cgagcggccg gcggcggaga
     5101 acccggccct ggcggcgcgg gggatcgcct cctaccgcga cctcaagacg ttcgtcgccg
     5161 accggccggg ccacgaccgg cgctacgcca tcgacgacga gaaggcgcgc accgagctcg
     5221 gctgggagac gcggcacgac ttcgcctccg gcctcgccgc caccgcctgc tggtacctcg
     5281 ccaacggcgc ctggtgccag gcggtgcagt ccggccgcta cgatcgcgag cgtctcggcc
     5341 tcctggaagg aaagcgatga agttcacccc cacccgcatc cccgaggtca tcctcgtcga
     5401 gcccgacgtg ttccgcgacg cccgcggcta cttcttcgag acccatcacc agaagaagta
     5461 cgccgaaggt gggatccccg agctcttcgt ccaggacaac cagtccttct cggtcgcccg
     5521 gacgctgcgc ggcctgcacg cccagctccg ccgcccgcag ggcaagctgg tgcgcgccat
     5581 ccagggggag atcttcgacg tcggcgtgga catccggccc ggctccccca ccttcggcca
     5641 gtgggtgggc gaggtgctgt cgggggacaa cttccggcag ctctacatcc cccccggctt
     5701 cgcccacggc ttctgcgtgc tgagcgagac cgccgtcgtg cactacaagt gcaccgacct
     5761 ctacgacaag gacgacgaga tcggcctcgt ctggaacgac ccggagatcg gcatcgagtg
     5821 gccgctggcc gatccgctgg tcaacgacaa ggacgggaac gcgccccgtc tggcagagat
     5881 cgtgcacaaa ctcaccctgc catgacgacg gggacgatga ccgagaccac gttcgcggct
     5941 ggtgacctcc agcagatgaa ggagctgg
//
LOCUS       DASPJI010000213         9265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_12151, whole genome shotgun sequence.
ACCESSION   DASPJI010000213 DASPJI010000000
VERSION     DASPJI010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9265)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9265
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12151"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VEL74_05640"
     CDS             complement(<1..547)
                     /locus_tag="VEL74_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62041.1"
                     /translation="MNSFRLAPRALARLAAAGLLILAAAALLPAPARADEASPFGVNI
                     HAPSGAELDQLLDRAQAAGIGWVRIDFVWAYVEGSRDRYDWSLYDEIAAEAQARGIEV
                     FATLAYTPDWATSGPGFTGVPDDPGQWADFCFRAARRYSGSIRYWGLWNEANLDKFWS
                     GTRQQYIDLIVKPGADAVHAGN"
     gene            688..1629
                     /locus_tag="VEL74_05645"
     CDS             688..1629
                     /locus_tag="VEL74_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62042.1"
                     /translation="MEFREITERYRLEKILKSSKSGTVLRAADGESGRTVVVKLIPVG
                     SAPNLEGRMGAFNRLAAAIGALGHPALPVVLDSGITPDGSAFLVMEHLDGRGLDTLAG
                     VAPSRALPLLMQAVSGLEALAGQRVPYLNLSPDNVLVIPGPAGDPVGEQAKLLGLGTV
                     LFQPVGGPGDEGRRFRAPELLSPQPGERADARADLYSLGVASCHVLGATIAPAAANGA
                     DPGVQMPLALSFELENAEALRQMLERCLRRRPAERPAHQAVRYAFQLALEGAESPLWQ
                     PAVPAPAEILQAPRPVEAPPPPVPVSAPVAAAPLPRP"
     gene            1656..3233
                     /locus_tag="VEL74_05650"
     CDS             1656..3233
                     /locus_tag="VEL74_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62043.1"
                     /translation="MAAAPVIPAPADPAPPIGPISPIGPIAAAPAPVVPDPEPLPEGD
                     VLSSVDDDLLAALAPTPPPPPPPGKAAKPPGSGRVLPFLGRSRPAPAPAPAAEGQAAA
                     PDEAVPAVPARGSRPLLLGVAAALLLIAALAVLWIMLREPGTAAPPPPVLAAAPRRPP
                     QPPAAERLEQARLLVAEGEDDRARQVLRTFTAADEAALPPSACRVLDGLRETLLLAAR
                     DRLPDDLTAGLEGDLARLRSAVDTGVEQPALVAALPPAAQAGFDRARRAMELYLQAEG
                     AARDGDHPAVLEHLGALVALAPQLADPEGLRAKSAEALEAEAGKLVEAGRYPEALAKL
                     EPLRRTWPERSGLAARVASYEGYQKDEAEQKRILEAIPAVERRRKPHEGLEVLAAVKP
                     TPHLAPEFAQARGRLENLLAQLDRQPPQVVLRDGYGLSYSRGSVVTLSFRATDDYEVK
                     SVKLMLRPPGGKMRSMAMETTRQGHTIEIPPSLHQNGTVEFYVTATDRSGHEGSLGTA
                     AQPMKLTREQGFERIVR"
     gene            3774..4094
                     /locus_tag="VEL74_05655"
     CDS             3774..4094
                     /locus_tag="VEL74_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62044.1"
                     /translation="MFSKIAHRSLLMVFVVALIVSPGVAQAGAFDPLSGRTLPSLGRF
                     QEQVQSWAELLWGSISGVWEKEGVSIDPNGAPKPGDSGTGGTSTGGTTSGEGGGSIDP
                     NGKP"
     gene            4256..5524
                     /locus_tag="VEL74_05660"
     CDS             4256..5524
                     /locus_tag="VEL74_05660"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62045.1"
                     /translation="MKDAHLSVETLARLLAGDLDHEELSNQVVPHLLARCPGCQRSYK
                     EIRRLQEEFAHWDERVAVFEGRQAPELFEQLAELPFDEQLSLVADDPSFQTWALCQLL
                     LKQSREARFYNPGMAVNLAELAVNISSNLGNAYDPHWVLDLQARTYAYLGSARRVLGE
                     LRSSDTAFRRAESFALRSMTGNSLVWAEILDLKSSLRREQRRGDEALSLLDEAISIYR
                     ESGDAHAISAALVKKAKILEESGDLTGAINLLQQAGDEIDPLRDARLFIYARHNLAWC
                     LTTAGRHEEAARLLPKVQDAFQQFAKPVDHIRLRWLEGRILFGLDNFETAEGVFREVQ
                     QQFLQRAMGFDAALVSLDLAILLAEQRRRPELKQLALEVIPVFEAQDVHREAMAALLM
                     FQKACNEETLTVELVRQIASTLEQNRQAKS"
     gene            complement(5600..8953)
                     /locus_tag="VEL74_05665"
     CDS             complement(5600..8953)
                     /locus_tag="VEL74_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020477946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYG62046.1"
                     /translation="MKRDSVLTGLLLSLLLLPGLAAAHPPEEEEKPPAPAPVAAAPPP
                     APTPQPAATAGTSAEQGKEEKKDKWDVNNPPGPSSEVTIDVDEGTWMSLDVSPDGKEI
                     VFDLLGDLYTIPIGGGEARALTHDVAWQMQPRYSPDGRHIAFTSDQGGGDNVWVMDRD
                     GGKPRQVTQESFRLLNSPDWTPDGEFLVARKHFTATRSLGAGEMWLYHRSGGEGLQLT
                     KKPNDQKDAGEPTFSPDGRYLYYSQDTTPGPLFEYNKDPNGQIYVIQRLDRETGEIEA
                     YVSGPGGAIRPTPSPDGKLLAFVRRVRGKSVLHLRDLRSGEEWPVHDGLDRDMQETWA
                     IHGVYPAMAWTPDSRSIVFWAGGKIRRLDLAKDGAGTVRDIPFHVRTTRRVQEALRFP
                     MEVAPERFPVRMLRWVEVSPKGDQVVYSALGQVWIRDLPEGKPRRLTRQDGHLEFYPS
                     FSRDGRSIVYVTWDDEELGSIRVAPADGGEGRVITREPGHYIEPAFTPDGKQVVYRRV
                     RGGGLRTTAWSGEPGIYRIPAGGGAATRITKDGTRPHFGADSDRVYLTRVGEKRELIS
                     IGLDRTDPRTHLSSENAIEFRVSPDGRWVAFNERFNAFIAPLAATGGSVDIGPKTKSI
                     PVARVSRDAGDYLRWSGDSRRLYWSLGPELYSRPLNEAFAFMEGAPEKLPDPPEKGID
                     IGFQLASDVPTGTVALVGARVITMRGDEIIEDGTVVIEGNRIRAVGPRAQVTVPAGAH
                     VIDARGKTVLPGLVDVHWHGSFGGDGLIPEQSWENAASLAFGVTTLHDPSNDNATIFT
                     AGEMARAGRILAPRVFSTGTILYGASSPNATATVDSLDDARSHLRRMKAIGAFSVKSY
                     NQPRREQRQQIVTAARELGMMVVPEGGSLYQHNMTMVVDGHTGIEHSLPVARIYDDVV
                     QLWKATEVGYTPTLVVAYGGPFGETYWYQHTKVWEDERLLSFVPREVIDPVARRPLVM
                     APEEEFNHIQVARVVKQLSDAGVGVQMGAHGQREGLGAHWEIWMLAQGGMTPHHALRA
                     ATIDGARYLGLDRDIGSIEPGKLADLAVLDADPLADIRNSRSVGLVVLNGRVYDARTL
                     DQTGNHPKKREPFYWQRQKPAFTTAPGEP"
     gene            complement(9080..>9265)
                     /locus_tag="VEL74_05670"
     CDS             complement(9080..>9265)
                     /locus_tag="VEL74_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62047.1"
                     /translation="VESFDVREDPENILAAEGKRRAPRRLIQTSFGLEVGETIVVGTS
                     RLDGGEQGLVVLLTAVS"
BASE COUNT         1319 a         3231 c         3278 g         1437 t
ORIGIN      
        1 ggttgccggc gtggaccgcg tcggccccgg gcttgacgat gaggtcgatg tactgctgcc
       61 gggtgcccga ccagaacttg tcgaggttcg cctcgttcca cagcccccag tagcggatcg
      121 agccgctgta gcgccgggcg gcccggaaac agaaatcggc ccactgcccg gggtcgtccg
      181 gcacgccggt gaagccgggg ccggaggtcg cccagtccgg agtgtaggcg agggtggcga
      241 agacctcgat cccccgggcc tgggcctcgg cggcgatctc gtcgtacagc gaccagtcgt
      301 agcggtcgcg cgagccctcg acgtaggccc agacgaagtc gatccgcacc cagccgatgc
      361 ccgcggcctg cgcccggtcg agcaactggt ccagctcggc gccggagggg gcgtggatgt
      421 tgacgccgaa cggcgaggcc tcgtcggccc gcgccggcgc cggcaggagg gcggcggcgg
      481 ccaggatcag gaggccggcc gctgcgagcc gggcgagagc gcgtggagcg agtcgaaagc
      541 tgttcatcat gtccgccaac cctcgttgcc ggctccccag tccatccgga tcgggcgggg
      601 agccgcttca gaggatagcc tatcgtctat actggtttcg tctgaacgct gagaacgtgc
      661 cgggtgtagg ccagggggag gcccgaggtg gagttccgtg aaatcaccga gcgctaccgg
      721 ctcgagaaga tcctgaagtc gtccaagtcc gggacggtgc tgcgcgccgc cgacggggag
      781 tcgggccgga ccgtcgtcgt caagctgatc ccggtggggt cggcccccaa cctggagggg
      841 cggatggggg ccttcaaccg gctggccgcc gccatcggcg cgctcggcca cccggcgctg
      901 ccggtggtcc tcgactccgg catcaccccg gacggcagcg ccttcctggt gatggagcac
      961 ctggacggcc gcggcctcga caccctggcc ggcgtcgccc cgagccgcgc cctgccgctg
     1021 ctgatgcagg cggtgagcgg cctcgaggcg ctggcgggcc agcgggtccc ctacctcaac
     1081 ctgtcgccgg acaacgtgct ggtgatcccc ggccccgccg gcgatcccgt gggcgagcag
     1141 gccaagctcc tggggctcgg caccgtcctc ttccagccgg tcggcggacc gggggacgag
     1201 gggcgccgct tccgcgcgcc ggagctgctg tcgccgcagc ccggcgagcg cgccgacgcg
     1261 cgggccgacc tctactccct gggcgtcgcg agctgccacg tgctgggcgc caccatcgcg
     1321 cctgccgcgg ccaacggggc ggatccgggg gtgcagatgc ccctggccct gagcttcgag
     1381 ctggagaacg ccgaggcgct gcgccagatg ctcgagcgct gcctgcgccg ccggcccgcg
     1441 gagcgcccgg cgcaccaggc ggtgcgttac gcgttccagc tcgccctcga aggcgccgag
     1501 tcccccctct ggcagccggc cgtgcccgcg ccggcggaga tcctccaggc ccccaggccc
     1561 gtggaggcgc cgccgccccc cgtaccggtc agcgcgcccg tcgccgccgc gccgctcccc
     1621 cgcccgtagc gccgccgccg gcgccggcga gcctcatggc cgcggcgccg gtgatccccg
     1681 cgccggccga cccggcgccc ccgatcggcc cgatcagccc gatcggtccg atcgcggcgg
     1741 cgcccgcccc cgtggtgccg gaccccgagc ccctccccga aggggacgtg ctgtcctccg
     1801 tcgacgacga cctgctggcg gcactggccc ccaccccccc gccgcctccg ccccccggca
     1861 aggccgcgaa gccgcccggc agcggccggg tgctgccgtt cctggggcgg tccaggcctg
     1921 ctccggcgcc ggctccggcg gccgaggggc aggccgcggc cccggacgag gccgtccccg
     1981 ccgttccggc ccgcggcagc cgcccgctcc tcctgggcgt ggccgcggcg ctgctcctga
     2041 ttgccgccct ggccgtcctc tggatcatgc tgcgggagcc cggcacggcc gcgccgccgc
     2101 cgccggtgct ggccgcggcc ccgcggcggc ccccgcagcc acccgccgcg gagcgcctgg
     2161 aacaggcccg gctcctggtg gccgaggggg aggacgaccg cgcccggcag gtcctgcgca
     2221 ccttcacggc cgccgatgag gcggcgctgc cgccgtccgc ctgccgggtg ctcgacggcc
     2281 tgcgcgagac gctgctcctc gccgcccgcg accgcctccc cgacgacctg accgccggcc
     2341 tggaggggga cctggcgcgg ctgcgctcgg cggtggacac cggcgtcgag cagccggcgc
     2401 tggtcgccgc cctgcctccc gcggcgcagg ccggcttcga ccgcgcccgc cgcgccatgg
     2461 agctctacct gcaggcggag ggcgccgccc gcgacggcga tcacccggcg gtgctcgagc
     2521 acctgggcgc cctcgtcgcg ctggcgccgc agctcgccga tcccgagggg ctgcgggcga
     2581 agtcggccga ggcgctggag gccgaagccg ggaagctggt cgaggccggc cgctatcccg
     2641 aggcgctggc gaagctggag ccgctgcgcc gcacctggcc ggagcgttcc gggctggcgg
     2701 cccgggtggc gagctacgag ggctatcaga aggacgaggc cgagcagaag cggatcctcg
     2761 aggcgattcc cgccgtggaa cgccgccgca agccgcacga ggggctggag gtcctcgccg
     2821 cggtgaagcc gacgccgcac ctggcgcccg agttcgccca ggcgcgcggc cggctggaga
     2881 acctcctcgc ccagctcgac cggcagcccc cgcaggtggt gctgcgcgac ggctacggcc
     2941 tgtcctactc gcggggctcc gtggtcaccc tgagcttccg cgccaccgac gactacgagg
     3001 tcaagagcgt caagctgatg cttcggccgc cgggcggcaa gatgcgctcc atggcgatgg
     3061 agacgacgcg ccaggggcac acgatcgaga tcccgccgag cctgcaccag aacggcacgg
     3121 tggagttcta cgtcaccgcc accgaccgct ccggccacga gggctcgctc ggcaccgccg
     3181 cccagccgat gaagctcacc cgcgagcagg gcttcgagcg gatcgtgcgc taggccctcc
     3241 cccccggcga atcggacgcg atccgcggcg tccgggaagc atccacgccg tctggactcc
     3301 atcttatcgg cctggcttcg ttccgaggcg cccggatgct ttccggagcg tctggaatcc
     3361 atcctgttga atcgggagcc atccgcgacg ttcgggaagg acccatagcg tctggacggc
     3421 ctccgcgacg cctggacatg atccaccgcg tctggagggc gagcccccgg gatggagctg
     3481 tctcctcccc tcctgcgacc cagagatacc acctgcgagg gaagagaatg agggtcccga
     3541 tcgcggcaat acgcgatcct cctctcccct cttggcatct tctcaggaat cgtggtcccg
     3601 aagtgggagt ccgccatttt cgattggagg ctgattccat cggcccttgc tcacgctctt
     3661 ttcttctccg gtggtgtccc gtttatatcc ggtaagggaa catcctgtaa cgagagcagg
     3721 cgctgccggc gccgtagccg gtgtcgcgtc ctgcgtcccg aaaggatcga atcatgttct
     3781 cgaagatcgc gcatcgttct cttctgatgg ttttcgtcgt ggctctcatc gtatcccccg
     3841 gcgtggcgca ggccggcgct ttcgatcccc tgagtgggcg gacgcttccc tcgctcggcc
     3901 gcttccagga gcaggtgcag agctgggccg agctgctctg gggcagcatt tccggagtct
     3961 gggagaagga gggagtctcg atcgatccca acggcgctcc caagccgggg gactccggca
     4021 ccggcggcac ctcgacgggc ggaaccacct ccggagaggg cggaggctcc atcgacccga
     4081 acggcaagcc ttgaaaggac cggcttcgcg ggacggggcc ggcacggatc ggccccatct
     4141 ttcctgaaga atcgtgtccc gaatcctcgc ttcccggtaa ctgaagtgga tagagggtat
     4201 atccttgtcc cgcgggagcc gtcctgcgtc cacattcgag aggaggaggg atatcgtgaa
     4261 agacgcccat ctgtcggttg agaccctggc ccgccttctg gcgggtgatc tcgaccacga
     4321 ggagttgtcg aaccaagtcg tgccgcacct gctggcccgc tgtcccggct gccaacggag
     4381 ctacaaagaa atccggcgct tgcaggagga gttcgcgcac tgggacgaga gggtggccgt
     4441 ctttgagggc cggcaggctc cggagctctt cgaacagctg gcagagcttc cgtttgacga
     4501 gcaactgagc cttgtcgccg acgatccaag cttccagacg tgggctcttt gtcagcttct
     4561 cctcaagcag agccgagaag cgcgcttcta caaccccggt atggccgtga acctcgctga
     4621 gctagccgta aacatctctt caaatctggg aaacgcctat gacccgcatt gggtcttgga
     4681 tcttcaagcg cgaacgtacg cctaccttgg gagcgcccgg cgagttctgg gagagctacg
     4741 gagcagtgat accgcgtttc ggagggctga gtcctttgcg cttcgtagca tgacggggaa
     4801 ctctctggtc tgggctgaga tcctcgatct caagagttcc cttcggcgcg agcaacgccg
     4861 cggcgacgag gcattgagcc ttctcgacga ggcgatctcc atctacaggg agagtggcga
     4921 tgcccacgcg atctctgccg cgctcgtcaa gaaagcgaag attctggaag agagcggcga
     4981 ccttactggc gcgatcaacc tgctgcaaca ggcaggcgat gagatcgacc ctctccggga
     5041 cgcccgtctg ttcatctacg cgcgccacaa tctggcctgg tgtttgacta cggcgggccg
     5101 tcatgaggag gcggctcgcc tcctgcccaa ggttcaagat gccttccagc agttcgcaaa
     5161 acccgtggat cacatccggc tccgctggct ggagggacga attctgttcg ggttggacaa
     5221 cttcgaaacc gccgaaggag tcttccggga agtccagcag caattccttc agcgagctat
     5281 gggcttcgac gcagctctgg tctccctcga tctcgcaatt ctcctggccg aacaacgccg
     5341 ccgcccagag ctcaagcagc tcgcgctgga ggtaatcccg gtcttcgagg cgcaggatgt
     5401 ccaccgcgaa gccatggccg ctctcctcat gttccagaag gcctgcaacg aggagacgct
     5461 caccgtggag ctggtgcggc agattgcctc gactctggag cagaaccggc aggcgaagag
     5521 ctagctttca gaaggactca gagggccaaa gctgagccgg tcccttgggg aggggaccgg
     5581 ctcactggct ttttgggggc tacggctcgc cgggcgcggt ggtgaaggcc ggcttctggc
     5641 gctgccaata gaacggctcg cgcttcttcg gatggttgcc ggtctggtcg agggtgcggg
     5701 cgtcgtagac gcggccgttc agcaccacca gcccgacgct cctggagttg cggatgtcgg
     5761 ccagcggatc ggcgtcgagc acggcgaggt cggcgagctt gccgggctcg atggagccga
     5821 tgtcacggtc gagccccaga tagcgggcgc cgtcgatggt ggcggcacgg agcgcgtggt
     5881 gcggcgtcat ccccccctgg gccagcatcc agatctccca gtgcgccccc agcccctcgc
     5941 gctggccgtg cgctcccatc tgcaccccga cgccggcgtc actgagctgc ttgacgacgc
     6001 gcgccacctg gatgtggttg aactcctcct cgggcgccat caccagcggc cggcgggcca
     6061 ccggatcgat gacctcgcgc ggcacgaagg aaagcagacg ctcgtcctcc cagaccttgg
     6121 tgtgctgata ccagtaggtc tcgccgaagg gcccgccgta ggcgacgacc agagtcgggg
     6181 tgtagccgac ctcggtcgcc ttccagagct ggaccacgtc gtcgtagatg cgggccacgg
     6241 gcagcgagtg ctcgatgccg gtgtggccgt cgacgaccat cgtcatgttg tgctggtaga
     6301 gcgatccacc ctccggcacc accatcatgc cgagctcgcg ggcggcggtc acgatctgct
     6361 ggcgctgctc gcgccggggc tggttgtagc tcttgacgct gaacgcgccg atggccttca
     6421 tccgccgcag gtgcgagcgg gcgtcgtcca ggctgtcgac cgtggccgtg gcgttgggcg
     6481 agctggcgcc gtagaggatg gtaccggtcg agaagacgcg cggcgccagg atgcggccgg
     6541 cacgcgccat ctcaccggcg gtgaagatcg tcgcgttgtc gttcgacggg tcgtgcagcg
     6601 tggtgacgcc gaaggcgagc gaagcggcgt tctcccagct ctgctcgggg atgaggccgt
     6661 cgccgccgaa cgagccgtgc cagtggacgt ccacgagccc cggcaggacc gtcttgccgc
     6721 gcgcgtcgat cacgtgggca cccgccggta ccgtcacctg ggcgcgcggc ccgacggcgc
     6781 ggatgcggtt gccctcgatc acgaccgtgc catcctcgat gatctcgtcg ccgcgcatgg
     6841 tgatcacgcg ggcgccgacc agcgccacgg tgccggtggg gacgtcgctc gccagttgga
     6901 agccgatgtc gatccccttc tccggcggat ccgggagctt ctcgggcgcc ccctccatga
     6961 aggcgaacgc ctcgttcagg ggacgtgagt agagctcggg gccgagcgac cagtagaggc
     7021 gccgcgagtc gcccgaccag cgcaggtagt cgccggcatc ccgcgacact ctggccaccg
     7081 ggatcgactt cgtcttcggc ccgatgtcca ccgaccctcc ggtcgccgcc agcggggcga
     7141 tgaaggcgtt gaaccgctcg ttgaaggcga cccaccggcc gtccggcgag acccggaatt
     7201 cgatggcgtt ctcgctggag agatgggtgc gtgggtccgt gcggtcgagg ccgatggaga
     7261 tcagctcgcg cttctcaccg acccgggtca ggtagacccg gtcgctgtca gcgccgaagt
     7321 ggggccgggt gccgtccttc gtgatgcggg tggcggctcc gccgccggcc gggatccggt
     7381 agatgccggg ctcgcccgac caggccgtgg tgcggaggcc gcccccccgg acccggcgat
     7441 agaccacctg cttgccgtcc ggcgtgaagg ccggctcgat gtagtgcccc ggctcgcggg
     7501 tgatcacccg cccctctcca ccatcggcgg gcgccacccg gatgctaccg agctcttcgt
     7561 cgtcccaggt gacgtagacg atggagcggc cgtcgcggga gaacgacggg tagaactcca
     7621 gatggccgtc ctggcgggtg agccgccgcg gcttcccctc cggcaggtcg cggatccaga
     7681 cctggccgag ggcggagtag accacctggt cccccttggg cgagacctcg acccagcgca
     7741 gcatgcggac cgggaaccgc tccggcgcca cctccatcgg gaagcgcagc gcctcctgga
     7801 cccggcgggt ggtgcggacg tggaagggga tgtcgcggac ggtgccggcc ccgtccttcg
     7861 ccaggtccag acggcggatc ttgccgccgg cccagaagac gatggagcgg gagtcggggg
     7921 tccaggccat cgccggatag acgccgtgga tcgcccaggt ctcctgcatg tcgcggtcga
     7981 ggccgtcgtg gaccggccac tcctcgcccg agcgcaggtc ccgcaggtgg aggacgctct
     8041 tgccgcgcac ccggcggacg aaggcgagca gcttgccgtc gggcgacggg gtggggcgga
     8101 tggcgccgcc cgggccgctg acgtaggcct cgatctcgcc ggtctcgcgg tcgagccgct
     8161 ggatgacgta gatctggccg ttcgggtcct tgttgtactc gaagagcggg ccgggcgtgg
     8221 tgtcctggct gtagtagagg tagcgcccgt cgggagagaa ggtcggctcg ccggcgtcct
     8281 tctggtcgtt gggcttcttg gtgagctgca ggccctcgcc gccgctgcgg tgatagagcc
     8341 acatctcgcc ggcgccgagg gagcgggtgg cggtgaagtg cttgcgcgcc accaggaact
     8401 cgccgtccgg cgtccagtcg gggctgttga gcagccggaa gctctcctgg gtcacctggc
     8461 gcggcttgcc gccgtcgcgg tccatcaccc agacgttgtc accgcccccc tggtcgctgg
     8521 tgaaggcgat gtgccggccg tcggggctgt agcgcggctg catctgccag gccacgtcat
     8581 gggtgagggc ccgggcctcg ccgccgccga tggggatggt gtaaaggtcg ccgagcaggt
     8641 cgaagacgat ctccttgccg tcggggctga cgtcgaggct catccaggtc ccctcgtcga
     8701 cgtcgatcgt cacttcagag gaagggccgg ggggattgtt gacgtcccac ttgtctttct
     8761 tctcctcctt cccctgctcg gcgctcgttc cagccgtggc cgcgggctga ggggtaggag
     8821 ccggcggcgg cgccgcggcg accggggcgg gcgcgggcgg cttctcctct tcttccggcg
     8881 ggtgtgccgc cgccaacccc ggcagcagca ggagggacag tagaagaccg gtcagaacgg
     8941 aatcgcgttt catcaagctc tttcctcgtc aggtcgatgg gcctgaagag taacagcatc
     9001 gcggggagcc gggattccgg gagggggacg agcacaccgg gccgggggat cgtccgccgg
     9061 cccggtcggg cagagggcct caggagacgg ccgtcaggag cacgaccagc ccctgttcgc
     9121 cgccatcgag cctggaggtg ccgaccacta tagtctcccc cacctccagg ccgaaggagg
     9181 tctggatcag ccgccgcggc gcgcgtctct tgccctcggc ggcgaggatg ttctcggggt
     9241 cctccctcac gtcgaagctt tcgac
//
LOCUS       DASPJI010000214        13134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_245086, whole genome shotgun sequence.
ACCESSION   DASPJI010000214 DASPJI010000000
VERSION     DASPJI010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13134)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13134
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245086"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="VEL74_05675"
     CDS             <1..1092
                     /locus_tag="VEL74_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate kinase"
                     /protein_id="HYG62048.1"
                     /translation="ERVTEKLRREPVEDFRADFEDGYGNRPDAEEDGHAGTAAREAAA
                     GLAAGTLPPFIGIRIKPFNEELRERSLRTLDLFLTTLVRETGGRLPENFVVTLPKVTI
                     PEQVTALADLFDLLEPALGLPAGSLRLEIMIEAPQAILDSEGRSPLPRLAEAARGRCV
                     AAHFGTYDYTASCNITAEHQSMTHPVCDFAKHMMQVAFAGTGLWISDGATNVLPVPPH
                     RPQGAAKDGPPLTQAQIDENRSVVHRAWRLHAAHVRHSLVGGFYQGWDLHPAQLVTRY
                     AALYSFFLEGLDAASDRLRNFVQKAAQATLVGEVFDDAATGQGLLNYFLRAVNCGAIT
                     EEEALERSGLTLEELRGRSFVKILQNRRG"
     gene            complement(1107..1949)
                     /gene="pucL"
                     /locus_tag="VEL74_05680"
     CDS             complement(1107..1949)
                     /gene="pucL"
                     /locus_tag="VEL74_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HYG62049.1"
                     /translation="MPVTLVDNSYGKQSVRLVTVRRGAERHVLKDVTAAIRFEGDFTA
                     AYTEGDNREVLPTDTMKNTVYALAAQHPFEDIEDFGLALTDHLLESSPRASAVRVELD
                     EHLWEPIEAGGTPHPHGFRKAGSERRTAVVTRDRHGARVLAGLEGLVVMKTAKSAFEG
                     FPRDRYTTLRETSDRLLATAVRATWRYSRPYVAFGRLWQEVRRVLLESFAAHDSLSVQ
                     HTLYAMGEAVLETVDIEEIHIAMPNLHHLPVDLTPLGLENRNEIFVATAEPYGLIEAT
                     LRRG"
     gene            complement(1953..2294)
                     /gene="uraH"
                     /locus_tag="VEL74_05685"
     CDS             complement(1953..2294)
                     /gene="uraH"
                     /locus_tag="VEL74_05685"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HYG62050.1"
                     /translation="MSGITTHVLDTSRGRPASGVPVLLAVLSEPGWREVGRAETDADG
                     RARQLLPAGSELSAGTYRLTFAIETYFQAQGIEGFYPEASIVFHVRDAAQHYHVPLLL
                     SPYGYSTYRGS"
     gene            complement(2291..2806)
                     /gene="uraD"
                     /locus_tag="VEL74_05690"
     CDS             complement(2291..2806)
                     /gene="uraD"
                     /locus_tag="VEL74_05690"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HYG62051.1"
                     /translation="MNLERLNLLPEDQVEAAFLSCCGSRAWARRMAMSRPFWGIDDIL
                     VNSDHIWWSLGREDWLEAFAAHPQIGEKVPEKPVAAGGEQARQWSVGEQKGTRGVAPE
                     ILDKLARGNLAYRKKFGYIFIVCATGKGAKQMLDILRERFDNDPETELRVAAEEQRKI
                     TNLRLKKMVKR"
     gene            complement(2803..3819)
                     /gene="alc"
                     /locus_tag="VEL74_05695"
     CDS             complement(2803..3819)
                     /gene="alc"
                     /locus_tag="VEL74_05695"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HYG62052.1"
                     /translation="MTDFTELVDLAAERLGGAVLAANDEFFAPKENLLKAGEAVWIEG
                     KYTDVGKWMDGWETRRRREPGHDWCLVRLGLPGVLRGVIVDTSHFRGNYPEACSLETC
                     SAAGHPTPEELSRDDVVWTEVLPRVTLQGNAKNPFPIERDAGRVTHLRFRIYPDGGVA
                     RLRVHGEVVPDWARLARFGVEVDLASVENGGLVIAASDMFFGSRHSLIVPGPPHGMHD
                     GWETRRRRGPGHDWALVKLGTRGAVHRIEVDTSHFKGNAPGSCSIEVCDTADLLPQAG
                     ADWREVLPRTPLRPHTLHRFDDVIETGPFTHARLNIHPDGGVARLRLFGRPVDSANPE
                     ESAR"
     gene            complement(3816..5183)
                     /gene="allB"
                     /locus_tag="VEL74_05700"
     CDS             complement(3816..5183)
                     /gene="allB"
                     /locus_tag="VEL74_05700"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HYG62053.1"
                     /translation="MEPGLVLQSERVVTPEGVRPATVHVSDGKILKVAGQADVPAGWP
                     LLRCGEQVVMPGIVDSHVHINEPGRTEWEGFRTATRAAAAGGITTLVDMPLNSIPATT
                     TREALRIKRAAAAGQCAVDAGFWGGVVPGNTRDLEGLLEDGALGFKCFLVPSGVDEFE
                     HVGETDLRQAMPVLARHGAVLLVHAELPGPIEAAAGVWGAAGLEELRQYERYLRSRPD
                     AAEVEAVELMIRLCRETGCRIHIVHVASAAVLPLLRAAREEGLPITAETCPHYLTFDA
                     EEIPDGGVAFKCAPPLRSRENRERLWEALRKNVIDMVASDHSPCPPEMKRIETGDFLH
                     AWGGVASLQVALPAVWTGARERGFTVADIAEWLCAAPARLAGLSGRKGSIAPGYDADL
                     VVWDPEASFTVDPARLRHRHAVTPYAGRTLYGAVRRTLLRGETVFGDLETGRASGTLL
                     TRSHG"
     gene            5294..6673
                     /locus_tag="VEL74_05705"
     CDS             5294..6673
                     /locus_tag="VEL74_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYG62054.1"
                     /translation="MMPTENSAAPAKPSRPLLLRGGTILTMDAGATVHRSGDILIRRG
                     RIAFVGRPVPPPETRILDISGCIVLPGLIQGHIHLGQTFFRGLAEQRRLLEWLRERIW
                     PLEAAHDDESAYWCGLLGAAECLLGGTTTIQDIGIGPGIRGLLRAITESGLRAFAGLC
                     LMDSGEGLPDALRQTTDRALAETEELGNAFDGSAGDRLRYVLNPRFILSCSDALWDGV
                     RDLSLRRGWPVHTHALEQKEETRAVREMKDGRDEIEYFADRGLLSTDLRLAHGVWLTK
                     SHLSWMRDRRVSVVHCPSSNLKLGSGIADLIAMRRFGVPAGLGCDGAACSNHLDSFEE
                     LRLAALLQKVKNGPESFTGLDALRLATSEGARALGLQEQTGSLEPGKFADLVILAPDR
                     PELWAAPQADPHDLVAFGASRAAVRHVFVEGRMLVRYGQLTRLDLPQIVRESNRCLAE
                     LIKRSGVKL"
     gene            complement(6681..7610)
                     /locus_tag="VEL74_05710"
     CDS             complement(6681..7610)
                     /locus_tag="VEL74_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYG62055.1"
                     /translation="MDSKRIGSLTVSTVGLGCNNFGTRLDAAATAEVVHAALDSGINF
                     FDTADIYGKGLSEEYLGRALGPRRAGVVVASKFGMVMDEHRKGAKPAYIRQAVEDSLR
                     RLGTDVIDLYQLHEPDPEVPLEETMGALNELVAAGKVREIGCSNFTAEQLRLSEEVAD
                     RTGTTRFVSVQNEYSLLHREPERGVLAECERLRIGFIPYFPLASGLLTGKYRRGRPVP
                     QGTRLSSSERFGKLLNDKNLTYVERLAEIGEARERTLLEIAFGWLLAKPVVVSVIAGA
                     TSPEQVRANAGAARRRLTDEELQEIEGVVAVPA"
     gene            complement(7701..9404)
                     /locus_tag="VEL74_05715"
     CDS             complement(7701..9404)
                     /locus_tag="VEL74_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HYG62056.1"
                     /translation="MRSTMDHFAPTWHRALLGALTILMAATPALADSTPLFSTYLGGS
                     RQDIGDAVAVQGGNTYVAGTTYSANYPVFMVSSSKPDEINNDAFVTALSSSGTPIYSR
                     YLPLGGGNEAVVGIGVTADGSAYVAGEAFAGGESYIVVVKLEPWGAVAWRRETYGRLS
                     AGGMTVDSQGNVYVTGRSTNPDENYDYRDYAYVWKLDASGATSYWAEIDGNYSEHGED
                     VAVDASGNAYVTGITISDNLPRSIQPAAPGGYNAFVTKLDPAGAILWSTYLGGIGDEF
                     GDQIEVAPDGSLVVAGTTNYSDFRTLNAIQAEHQGEQDLFLVRLRPWGSLISSTYLGG
                     SSNDSVTALALDASSILLAVSSSPEDGFPLPEPLDPSCGGMFVAKLDATASRVLDAAC
                     LGNSRIHGVAVDSSGVSLTGVTDDSLPLANAWQPAYGGNGDAFAAKLTLNNTPDCSAA
                     AASPATLSPADGRFVNVSIRGVTDPDGDRVTITLTSIFQDEFLTSPGTPDAGGVGTPT
                     ARLRASRIPGGDGRVYHLRYTATDTQGGTCSGTVKVCVPLSGGGTCVDGGARIDSTRA
                     Y"
     gene            complement(9456..11684)
                     /locus_tag="VEL74_05720"
     CDS             complement(9456..11684)
                     /locus_tag="VEL74_05720"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HYG62057.1"
                     /translation="MAWLLGGGGTLEARTLAFEPNLGQTDGRVKFLSHGDGYLLFLTP
                     QEATLALQGRDGTGRALRLRWVGASPAPGLSGEAPLRGRSHYMRGKDPARWYRDVPNY
                     GKVRYEEVYPGVDLLFYGREGRLEYDLLLEPGADPGLIRLAVDGADRLAIDVSGDLVA
                     SVGGEEVRFLRPFSYQETGGVRREVASRYRLLAGGEVGFAVDAHDPARPLVIDPVVLY
                     SVRFGGSGVDESAAVDVDTDGNTYHAGQTSSPDLPLAFGSSATVTDLSTHTFIADAFV
                     TKLDPAGEVVFSTYLGGDTIDEATDVAVSPVSGVYVSGISSSNDGRGGWVVHLDPLGS
                     PVYWAGPQTEGDTRSRATGVAVDSHGNAYLTGFNDYGARDAWAVKVDTEGQILYNAYL
                     GGEWGGIEPSGIAVDTAGNAWVTGTVWTSELPLVRPFQGTLKGPSDAFVVKIDPSGTV
                     VFSTYFGGGSWDDVKRIAAGPDGSVYLAGNTASTDFPTRQAMQPARREFQDLFLSRFS
                     PTGYLMTSTYLGSGGSDALLGLAVDRSGIVYLASAAGAGDSPLRDPALPDCRGPLLGS
                     LDRNLQAVLQTACLPGKTVRDIAANSPRLLYVTGQESGYLYDVFAAKLAFNRPPQCGG
                     AFASLATLWPPDGKLLPVTIRGVTDPERDPVTIAVASVFQDEFLTSPGTPDASGIGTS
                     TARLRASRLTGGDGRVYHLRFTATDTWGDTCSGTVKVCVPITEGGTCGDGGARIDSTR
                     SY"
     gene            11966..13087
                     /locus_tag="VEL74_05725"
     CDS             11966..13087
                     /locus_tag="VEL74_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HYG62058.1"
                     /translation="MPRKARVAVLKTKPETVLEDVGRTMELAEVKSHLAPGATTILKD
                     NISWHFPFPGANTTPWQLEGTILKLREDGYTDQVCVQNNTVVTDPKKGERLNRYLPVL
                     DKYDVPVRYNCFADDLKWIQYQPKGKMLVLPKIYPEGITIPEFFLGKNVVHLPTVKCH
                     IYTTTTGAMKNAFGGLLNTKRHYTHSYIHETLVDLLTIQKEIHSGLFAVMDGTTCGNG
                     PGPRTMIPVEKDVLLASGDQVAIDAVAAKMMGFDPMTIPYIRLAHDAGLGIGDPRDIE
                     IVGEDVSKWNFRFSVGDNAVSRFGDLVWFGPFKGLQKLLFRTPLVYFFVFGSYFYHDF
                     AWYPTVGKKRMEQYAKTSKWMRLFETYPEGSESQQEAAA"
BASE COUNT         2036 a         4525 c         4514 g         2059 t
ORIGIN      
        1 gagcgggtga cggagaagct tcgccgcgag ccggtcgagg atttccgggc cgacttcgag
       61 gacggctacg gcaaccgccc ggacgcggag gaggacgggc acgccgggac ggccgcccgc
      121 gaggcggccg cggggctcgc cgcggggacg ctgccgccgt tcatcggcat ccgcatcaag
      181 ccgttcaatg aggagctgcg cgagcggtcc ctgcgcaccc tggacctgtt cctgaccacg
      241 ctcgtccgcg agaccggagg gcggctgccg gagaatttcg tcgtcaccct gccgaaggtg
      301 acgatcccgg agcaggtgac cgcgctcgcc gacctcttcg atctcctgga gccggcactg
      361 ggcctgccgg ccggatcgtt gcggctcgaa atcatgatcg aagcaccgca ggcgatcctc
      421 gacagcgagg ggagatcccc cctgccgcgc ctcgccgaag ccgcgcgcgg ccggtgcgtc
      481 gccgcccatt tcggcaccta cgactacacc gcctcgtgca acatcacggc ggagcaccag
      541 tcgatgaccc acccggtctg cgacttcgcc aagcacatga tgcaggtcgc cttcgccggc
      601 accggcctgt ggatctcgga cggcgcgacc aacgtcctgc cggtcccgcc gcaccggccg
      661 cagggtgccg ccaaggacgg cccgccgctc acccaggcac agatcgacga gaaccggtcc
      721 gtggtccacc gcgcctggag gctgcacgcc gcgcacgtcc ggcactcgct cgtgggcggc
      781 ttctatcagg gttgggacct tcaccccgcg cagctcgtca cccgctatgc cgcgctgtat
      841 tcgttcttcc tggaagggct cgacgcggcc tccgatcgtc tccgcaactt cgtgcagaag
      901 gccgcgcagg cgacgctggt gggcgaggtc ttcgacgacg cggccaccgg ccaggggctc
      961 ctcaactatt tcctccgtgc ggtcaactgc ggtgcgatca ccgaggagga ggcgctggag
     1021 cgatcaggtt tgaccctgga agagctgcga gggcgttcgt tcgtcaagat cctgcagaac
     1081 cggcggggct gagaccctga gaccggtcag ccccgccgca gcgtcgcctc gatcaggccg
     1141 tagggctcgg cggtggcgac gaagatctcg ttgcggtttt ccaggccgag gggcgtgagg
     1201 tccaccggca ggtggtgcag gttgggcatg gcgatgtgga tctcttcgat gtccacggtc
     1261 tcgaggacgg cctcgcccat cgcatagagc gtgtgctgga ccgacaggct gtcgtgcgcc
     1321 gcgaacgact cgaggagcac gcgccgtacc tcctgccaga gccggccaaa ggcgacgtag
     1381 ggacgcgaat agcgccaggt ggcccgcacc gccgtcgcga ggagccggtc cgaggtctcg
     1441 cgcaaggtgg tgtagcggtc gcgcgggaaa ccttcgaaag ccgatttcgc ggtcttcatc
     1501 accaccaggc cttcgaggcc ggccagcacc cgtgctccat gccggtcccg cgtcaccacg
     1561 gccgtgcgcc gctcggagcc tgctttgcgg aagccatgcg gatgcggcgt tcccccggcc
     1621 tcgatgggct cccataggtg ctcgtcgagc tccacccgga cggccgacgc ccgcgggctg
     1681 ctctccagca agtgatcggt cagcgccagg ccgaagtctt cgatgtcctc gaaggggtgc
     1741 tgggcggcca gggcgtagac ggtgttcttc atcgtgtccg tggggaggac ctcccggttg
     1801 tcgccctcgg tataggcggc cgtgaaatcc ccttcgaacc ggatggcggc cgtgacgtcc
     1861 ttgagaacgt gccgctccgc gccgcgccgg accgtcacca gccgtactga ctgcttgcca
     1921 taactgttgt cgacgagcgt gacgggcatg aatcagctcc cccggtaggt cgaatatccg
     1981 tatgggctca gcaggagcgg cacgtgatag tgctgggccg cgtcccgcac gtggaagacg
     2041 atcgaggcct cgggatagaa accttcgatc ccctgcgcct ggaaataggt ttcgatcgcg
     2101 aacgtgagcc ggtaggtgcc ggcggagagc tccgagcccg cgggcaggag ctgccgggca
     2161 cggccgtcgg cgtctgtctc ggcgcgcccg acctcgcgcc agccgggttc tgacaggact
     2221 gcgagcagga ccggcacgcc gctcgccggc cggccgcgcg aggtgtccag aacatgggtg
     2281 gtgatgccgc tcatcgtttc accatttttt tgagccggag attggtgatc ttcctctgct
     2341 cctcggcggc gacccgcaac tcggtctcgg gatcgttgtc gaaccgctca cgcaggatgt
     2401 cgagcatctg cttggcgccc ttgccggtgg cgcagacgat gaagatgtag ccgaatttct
     2461 tgcgataggc gagattgccc cgggcgagct tgtccaggat ctcgggcgcc acgccgcggg
     2521 tccccttctg ttcgccaacc gaccactggc gggcctgctc gcctccggcc gcgacgggct
     2581 tctcgggcac cttctcgccg atctgcggat gggccgcgaa ggcctccagc cagtcctcgc
     2641 ggccgaggct ccaccagatg tgatcggaat tcaccaggat gtcgtcgatg ccccagaacg
     2701 gccggctcat ggccatccgg cgcgcccagg cccgcgagcc gcagcacgac aggaacgcgg
     2761 cttcgacctg gtcctccggc aacagattca atcgctccag attcaccgcg cgctttcctc
     2821 cgggttcgcc gagtccacgg gacgtccgaa gagccgcagg cgggccacgc cgccgtcggg
     2881 atggatgttg aggcgcgcgt gagtgaacgg tcccgtttcg atcacatcgt cgaaccggtg
     2941 cagggtgtgg gggcgcagcg gcgtgcgcgg caggacctcg cgccaatccg ccccggcttg
     3001 cggcaggagg tccgccgtgt cgcagacctc gatcgagcag gagccgggcg cgttgccctt
     3061 gaaatgggac gtatccactt cgatccggtg gaccgcgccg cgggtcccga gcttcacgag
     3121 ggcccaatcg tgccccggcc cccgacggcg ccgcgtctcc cagccgtcgt gcatgccgtg
     3181 cggcggcccg ggcacgatca ggctgtgccg cgagccgaag aacatgtcgc tcgccgcgat
     3241 caccaggccg ccgttctcca ccgacgccag atcgacctcg accccgaagc gggcgagccg
     3301 cgcccagtcg ggcaccacct cgccatgcac ccgcaggcgg gcgacgccgc cgtccggata
     3361 gatgcggaag cggagatggg tgacccggcc ggcgtcgcgc tcgatgggga agggattctt
     3421 cgcgttgccc tggagggtca cgcgggggag cacctcggtc cacaccacgt cgtcccgcga
     3481 cagctcctcg ggcgtcggat ggcccgctgc cgagcaggtt tcgagcgagc aggcctccgg
     3541 gtagttgccc ctgaagtggc tggtgtcgac gatcacgccc cgcagcactc ccggcagtcc
     3601 gaggcggacg aggcaccagt cgtgacccgg ttcccggcgg cggcgggtct cccagccgtc
     3661 catccatttg cccacatcgg tgtacttgcc ctcgatccag accgcctcgc ccgccttgag
     3721 gaggttctcc ttgggcgcga agaactcgtc gttcgccgcc aggaccgctc cgcccagccg
     3781 ctcggccgcg aggtccacca gctcggtgaa atcggtcatc catggctcct tgtgagcaac
     3841 gttccagaag cgcggccggt ctcgagatcc ccgaagaccg tctcaccccg cagcagcgtg
     3901 cggcggactg ctccgtaaag ggtccgtccc gcataggggg tgaccgcgtg acgatgcctg
     3961 aggcgcgccg ggtccaccgt gaagctcgcc tccggatccc ataccaccag gtcggcatcg
     4021 tatcccggag caatgctccc cttcctcccg gagaggccgg ccagccgcgc cggagcggcg
     4081 cacagccact ccgcgatgtc ggcgactgtg aagccgcgct cccgggctcc ggtccagact
     4141 gccggcagcg ccacctggag cgaagcgact ccaccccagg cgtgcaggaa gtcgccggtc
     4201 tcgatccgct tcatctccgg cgggcagggg gagtgatccg acgccaccat atcgatcaca
     4261 ttctttcgca gagcctccca caaccgctcc cggttctcgc gcgaccggag cggcggcgca
     4321 catttgaagg cgacgccgcc gtcggggatc tcctcggcgt cgaaggtcag gtagtgcggg
     4381 caggtctcgg ccgtgatcgg gagcccctcc tcgcgggcgg cccgcagcaa cggcagcacc
     4441 gccgccgagg cgacgtggac gatgtggatc cggcagcccg tctcacggca gagccggatc
     4501 atcagctcga ccgcctccac ctccgccgcg tccggccggg accgcaagta gcgctcgtac
     4561 tgccgcagct cctccaggcc cgcggctccc cagactccgg ccgccgcctc gatcggtccc
     4621 ggcagctcgg catggacgag aaggacggcc ccgtgccggg cgagcaccgg catggcctgc
     4681 cggaggtcgg tctcccccac atgctcgaac tcatcgacgc cggaggggac caggaaacat
     4741 ttgaacccga gagccccgtc ttcgaggagc ccttccaggt ccctcgtgtt gccgggcacc
     4801 acgccgcccc agaaccccgc atcgaccgcg cactgtcccg ccgcggccgc cctcttgatc
     4861 cgcagggcct cgcgggtggt cgtggccggg atgctgttga gcggcatgtc gaccagagta
     4921 gtgatcccgc cggccgccgc cgcccgcgtc gccgtccgga acccctccca ctcggtccgc
     4981 cccggctcat tgatatgaac gtggctgtcg acgatccccg gcatcacgac ctgctcaccg
     5041 caacgcagga gcggccagcc ggccgggacg tcagcttgcc cagccacctt caggatcttg
     5101 ccgtcggaga catggaccgt ggccgggcgc accccctccg gggtcaccac ccgttcgctc
     5161 tggaggacga ggcccggttc catggccggg acagtacgcc gtacccgcca ccctctgtca
     5221 actccggaga cgttgtcgaa tcggcgcggg cggcacgagg agtgcggaga cgttgtagga
     5281 tcacacgcag gtgatgatgc ccaccgaaaa ctccgcggct ccggccaagc cgtctcgccc
     5341 cctgctgctg cgcggcggca cgatcctgac catggatgcc ggcgccaccg tgcaccggag
     5401 cggcgacatc ctcatccgtc gcggccggat cgcgttcgtc ggccgtccgg tcccgccgcc
     5461 ggagacgcgg atcctcgata tctccggctg tatcgtcctg cccgggctca tccaggggca
     5521 catccacctg gggcagacct tcttccgcgg cctcgcggag cagcggcgcc tcctcgaatg
     5581 gctgcgcgag cgcatctggc cgctcgaagc cgcccacgac gacgagagcg cctactggtg
     5641 cggcctgctc ggcgcggccg agtgcctgct cggcggcacc accacgatcc aggatatcgg
     5701 gatcggaccg ggtatccgcg gcctgttgcg ggcgatcacc gagtcgggcc tgcgcgcctt
     5761 cgcgggcctc tgcctgatgg actccggcga ggggctcccc gacgccctgc ggcagacgac
     5821 cgaccgggcc ctcgccgaaa ccgaggagct gggcaacgcg ttcgacggct cggccggcga
     5881 ccggctgcgc tacgtcctca acccgcgttt catcctctcc tgctcggacg cgctctggga
     5941 cggcgtccgc gatctctcgc tgcgccgcgg ctggccggtc cacacccacg cgctggagca
     6001 gaaggaggag acccgcgcgg tacgcgagat gaaggacggg cgggacgaga tcgaatattt
     6061 cgccgaccgg ggattgctca gcaccgacct gcgcctcgcg cacggcgtct ggctgaccaa
     6121 gagccatctc tcctggatgc gggaccgccg cgtcagcgtc gtccactgcc cgagctcgaa
     6181 cctgaagctc ggctccggga tcgccgacct catcgccatg cgccgcttcg gcgtgccggc
     6241 cggcctcggc tgcgacggcg ccgcctgcag caatcatctg gacagcttcg aggagctgcg
     6301 cctcgcggcc ctcctgcaga aagtgaagaa cggtcccgag tcttttaccg gtctcgacgc
     6361 gctgcgtctg gcgacgagcg agggggcgcg cgccctcggc ctccaggaac agaccggcag
     6421 cctggagccg ggcaagttcg ccgacctggt catcctcgct ccggaccgcc ccgagctctg
     6481 ggccgccccg caggccgacc cacacgatct cgtcgccttc ggagcctccc gcgccgccgt
     6541 ccgccacgtc ttcgtggaag gccgcatgct ggttcgctac ggccagctca cccgcctcga
     6601 tctgccgcag attgtccggg aatcgaaccg ttgcctggcg gagctgatca agcggtcggg
     6661 ggtgaagctg tagcggcccc tcaggccggt accgcgacga ccccctcgat ctcctggagc
     6721 tcctcgtcgg tgagccgccg gcgcgcggct ccggcgttcg cccggacctg ctccggcgag
     6781 gtcgcgcccg cgatgacgga gacgacgacc ggcttggcca acagccagcc gaaggcgatc
     6841 tcgagcaagg tccgctcccg cgcttcgccg atctcggcga gccgctcgac gtaggtcagg
     6901 ttcttgtcgt tgagcagctt gccgaaacgc tcgctcgacg agaggcgggt tccctggggc
     6961 accggccggc ctctccggta cttgccggtg agcagaccgc tggcgagcgg gaagtagggg
     7021 atgaagccga tcctcaagcg ctcgcattcg gcgaggacgc cgcgctccgg ctcgcgatgg
     7081 aggaggctgt actcgttctg cacgctgacg aagcgcgtgg tgccggtgcg gtccgccacc
     7141 tcctcggaca gccggagctg ctcggccgtg aagttggagc agccgatctc gcgcaccttg
     7201 ccggccgcca ccagctcgtt gagtgcgccc atggtctcct ccagggggac ctcggggtcg
     7261 ggctcgtgga gctggtagag gtcgatgacg tcggtgccga gccggcgcag gctgtcctcg
     7321 accgcctgcc ggatgtaggc cggtttggca cccttccggt gctcgtccat caccatgccg
     7381 aacttgctgg ccaccaccac gccggcccgc cggggcccga gggcgcggcc gaggtactcc
     7441 tcgctcagcc ccttgccgta gatgtcggcg gtgtcgaaga agttgatccc ggagtccagc
     7501 gccgcgtgca cgacctccgc agtggccgcc gcgtcgaggc gtgtcccgaa gttgttgcag
     7561 cccagcccca ccgtggagac tgtgagtgac ccgatccgtt tgctgtccat gcttctctcc
     7621 ccttcccggt cagagtgtcc aagaaagctt gcagcaagag gggtgcccgt tcgcacgggc
     7681 accccggatc gcgtcctttt tcagtaggcc cgggtcgagt cgatccgcgc gccgccgtcg
     7741 acgcaggtgc cgcccccgct gagggggacg cagaccttga cggtgccgga gcaggtgccg
     7801 ccctgggtat cggtggcggt gtagcgcagg tgatagacgc ggccgtcccc tcccgggata
     7861 cgggacgcgc gcagacgggc ggtcggggtg cccactccgc cggcgtcggg cgtgccgggg
     7921 gaggtgagga actcgtcctg gaagatggag gtcaaggtga tggtaacgcg gtcgccgtcc
     7981 gggtcggtga cgccgcggat ggagacgttg acgaaccgtc cgtcggccgg cgagagcgta
     8041 gcagggctcg ccgcggcggc ggagcagtcc ggcgtgttgt tgagtgtcag cttcgccgcg
     8101 aaggcgtcgc cgttgccccc gtaggccggc tgccaggcgt tggccagcgg caggctgtcg
     8161 tcggtcacgc cggtcaggga gacgccggag gagtccacgg cgacgccgtg gatcctggag
     8221 ttgccgaggc aggcggcgtc caacacccgt gaggccgtgg cgtcgagctt cgccacgaac
     8281 atgccgccgc acgaaggatc gagcggctcc ggaaggggaa agccgtcctc cgggctgctg
     8341 ctgacggcca ggaggatgct cgaggcgtcc agggccagag ccgtcacgga gtcgttggag
     8401 cttcctccca gataggtgga ggagatgagg gagccccagg gcctcagccg caccaggaac
     8461 aggtcctgtt cgccttggtg ctcggcctgg atggcgttca gggtccggaa gtcggaatag
     8521 ttggtggtgc cggcgacgac cagcgagccg tccggcgcga cctcgatctg atccccgaat
     8581 tcatcgccga tcccgcccag ataggtcgac cacaggatgg cgccggccgg atccagcttg
     8641 gtcacgaaag cgttgtaacc gcccggggcg gccggctgga tcgacctggg gaggttgtcc
     8701 gagatggtga tgccggtgac gtatgcgttg cccgaggcgt ccacggcgac gtcctcgccg
     8761 tgctcggaat agttgccgtc gatctcggcc cagtagctcg tcgccccgct ggcgtcgagc
     8821 ttccagacgt acgcatagtc gcgatagtca tagttctcgt cggggttggt gctgcggccg
     8881 gtgacgtaga cgttgccctg ggagtccacg gtcatgcctc cggcgctcag ccggccgtag
     8941 gtctcacgcc tccaggcgac ggctccccag ggctccagct tgacgacgac gatgtacgac
     9001 tcgccccccg caaaggcctc gcccgccaca taggcgctac cgtcggcggt cacgccgatg
     9061 ccgactacgg cctcgttgcc cccgcccagg ggcaggtacc gcgagtagat gggagtcccg
     9121 gaggagctca gggcggtgac gaaggcgtcg ttgttgatct catccggctt gctggagctc
     9181 accatgaaga ccgggtagtt cgccgagtag gtggtgcccg caacgtaggt gttccccccc
     9241 tgcaccgcga cggcgtctcc gatgtcctgg cggctccctc ctagataggt cgagaagagg
     9301 ggggtggaat cggcaagggc cggcgtagcc gccatcagga tggtgagcgc gccgagcagc
     9361 gcgcggtgcc aggttggtgc gaagtgatcc atcgtgcttc tcatgatgac cctccatgga
     9421 atgagctgtg ctgggagagg catgtcgtca cctcatcagt aggaccgggt cgagtcgatc
     9481 cgcgcgccgc cgtcgccgca ggtgccaccc tcggtgatgg gcacgcagac cttcaccgtc
     9541 ccggagcagg tgtcgcccca ggtgtcggtg gcggtgaagc gcaggtgata gacgcggccg
     9601 tcgcctccgg tcagccggga ggcgcgcagg cgggcggtgg aggtgccgat gccgctggcg
     9661 tccggtgtgc cgggagaggt gaggaactcg tcctggaaga cggaggccac ggcgatggtg
     9721 acggggtccc tttccgggtc ggtgacgccg cggatcgtga cggggaggag cttgccgtcg
     9781 ggcggccaga gggtggcgag cgaagcgaag gcgccgccgc actggggtgg acggttgaag
     9841 gcgagcttcg ccgcgaagac gtcgtagagg tagccgctct cctggccggt gacgtagagg
     9901 agccgcggag agttggcggc gatgtcgcgc acggtcttgc cgggcaggca ggcggtctgg
     9961 agaacggcct ggaggttgcg gtccaggctc cccaggaggg ggcccctgca gtcggggagg
    10021 gccgggtcgc gcaggggcga gtcgccggca ccggcagcac tcgccagata gacgatgccc
    10081 gagcgatcca ccgccaggcc gaggagcgcg tcgctgccgc cgctcccgag ataggtcgag
    10141 gtcatcaggt agcccgtggg actgaaccgg ctcaggaaca ggtcctgaaa ttcccggcgg
    10201 gccggctgca tcgcctggcg cgtgggaaag tcggtagagg ccgtgttccc ggccaagtag
    10261 acgctaccgt ccggcccggc cgcgatgcgc ttcacgtcat cccaggagcc accgccgaaa
    10321 taggtggaga agacgaccgt tccggacggg tcgatcttca ccacgaacgc gtccgacggt
    10381 cctttcaggg taccctggaa ggggcggacc agggggagct cggaggtcca taccgtgccg
    10441 gtcacccagg cgttgcccgc agtatccacg gcgatccccg agggctcgat gcctccccac
    10501 tcgccaccga ggtaggcgtt gtaaaggatc tgcccctcgg tgtctacttt cacggcccat
    10561 gcatcccggg ctccgtagtc gttgaatccc gtgagatagg cgttcccgtg cgagtcgacc
    10621 gccacccccg tggcccggct gcgggtatcg ccctcggtct gcggtcctgc ccagtagacg
    10681 gggctgccga gggggtcgag gtggacgacc catccacccc tgccgtcgtt gctggagctg
    10741 ataccgctca catagacgcc ggaaacgggg ctgacggcga cgtctgtcgc ctcgtcgatg
    10801 gtgtcgcctc caaggtaggt ggagaagacg acctcaccgg cgggatcgag cttggtgacg
    10861 aaggcgtcgg cgatgaaggt gtgagtgctc aagtcggtga ccgtggcgga cgagccgaac
    10921 gccagaggca ggtcgggcga gctcgtttgg ccggcgtggt aggtgttgcc gtccgtgtcc
    10981 acgtcgaccg cggcgctctc gtccacgccg ctgccgccga agcggaccga gtagaggacg
    11041 accgggtcga tcaccagcgg ccgcgccggg tcgtgggcgt ccacggcgaa gcccacctcg
    11101 ccgccggcga gcaggcgata gcggctcgcc acctcgcggc ggacgccgcc ggtctcctgg
    11161 taggagaagg gccgcaggaa ccgcacctcc tcgccgccga ccgaggcgac gaggtcgccg
    11221 gacacgtcga tcgcgaggcg gtcggcgccg tcgacggcga gacggatcag gccgggatcg
    11281 gcgcccggtt ccaggaggag gtcgtactcg agcctcccct cccggccata gaagaggaga
    11341 tcgacgcccg ggtagacctc ttcatagcgc accttgccgt agttcggcac gtcgcggtac
    11401 cagcgggcgg ggtccttccc ccgcatgtag tggctcctgc cgcgcagcgg cgcctcgccg
    11461 gataggccgg gcgccggaga cgcccccacc cagcgcaggc gcagcgccct gccggtgcca
    11521 tcgcggccct gcagggcgag cgtcgcctcc tgaggagtca ggaacaaaag gtatccgtca
    11581 ccgtgggaca ggaacttcac ccggccgtcg gtctggccga ggttgggctc gaaggccagc
    11641 gtccgcgcct ccagggtccc ccctccgccc agcagccatg ccattgcgag tgcggctccg
    11701 acgattctcc ttgtcctgct tctcaagatg tcctcctttg ccggccgtca tggatggaac
    11761 cccatccccg tggggaatag ggaagtacgc atgtaagttc gggaaccata gggaagcggc
    11821 ggcagcccgc ctcccgcgaa agggtctctt ccgagactgc gaagggcgtc acgcttcagt
    11881 tttgctgcaa gaacggttcc ggcggaggcc gtgggcggag cctgctagaa tgctttcggc
    11941 tatattgaag gatggagagt tttcaatgcc caggaaagcg cgcgttgccg ttttgaagac
    12001 caagcccgag acggtcctgg aggacgtcgg gcgcaccatg gagctggcgg aggtcaagag
    12061 ccacctcgct ccgggcgcga ccacgatcct caaggacaac atctcgtggc atttcccgtt
    12121 ccccggggcg aacaccaccc cctggcagct cgaggggacc atcctcaagc tgcgcgagga
    12181 cggctatacg gaccaggtct gcgtccagaa caacacggtg gtcaccgacc ccaagaaggg
    12241 ggagcggctg aaccgttacc tgccggtgct cgacaagtac gacgtgccgg tgcggtacaa
    12301 ctgcttcgcc gacgacctca agtggatcca gtaccagccc aaggggaaga tgctggtgct
    12361 gcccaagatc taccccgagg ggatcaccat ccccgagttc ttcctgggca agaacgtcgt
    12421 ccacctgccg acggtcaagt gccacatcta tacgaccacc accggcgcga tgaagaacgc
    12481 cttcggcggc ctgctcaaca ccaagcgcca ctacacccac tcctacatcc acgagacgct
    12541 ggtcgacctg ctgaccatcc agaaggagat ccactccggc ctcttcgcgg tgatggacgg
    12601 cacgacctgc ggcaacggcc ccgggccgcg caccatgatc ccggtggaga aggacgtcct
    12661 cctcgcctcg ggcgaccagg tggcgatcga tgccgtggcc gccaagatga tgggcttcga
    12721 cccgatgacg atcccctaca tccggctggc gcacgacgcc gggctgggga tcggcgaccc
    12781 gcgcgacatc gagatcgtgg gcgaggacgt ctcgaagtgg aacttccgtt tctcggtggg
    12841 ggacaacgcg gtctcccgct tcggcgacct ggtctggttc ggcccgttca agggcctcca
    12901 gaagctgctc ttccggacgc cgctcgtcta cttcttcgtc ttcggctcgt acttctacca
    12961 cgacttcgcc tggtacccga ccgtgggcaa gaagcgcatg gagcagtacg ccaagaccag
    13021 caagtggatg cgcctgttcg agacctaccc cgaggggagc gagagccagc aggaggccgc
    13081 ggcctgatcc ggaccccggc ggccgcgtgc cggtgatccc gctcgggacc cggc
//
LOCUS       DASPJI010000215         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_304985, whole genome shotgun sequence.
ACCESSION   DASPJI010000215 DASPJI010000000
VERSION     DASPJI010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304985"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VEL74_05730"
     CDS             complement(<1..485)
                     /locus_tag="VEL74_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYG62059.1"
                     /translation="MPHSVPLITTIAAGLGLALILGFIAVRLKVPAIVGYLVAGIVIG
                     PATPGFVADVGLASQLAEIGVMLLMFGVGLHFSFSDLLEVRKIALPGAIVQSAVATAL
                     GAGVATLWGWSLPAGIAFGLCLSVASTVVLLRALESRGILDTVNGRIAIGWLIVEDIF
                     TV"
     gene            671..1315
                     /gene="miaE"
                     /locus_tag="VEL74_05735"
     CDS             671..1315
                     /gene="miaE"
                     /locus_tag="VEL74_05735"
                     /inference="COORDINATES: protein motif:HMM:NF017939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HYG62060.1"
                     /translation="MRRADVNERDSTEPMGFEEIYAELPLLHPTPRSWAELAAARLPE
                     FLADHAVCEQQVAMYALSLAGYYPDDAELVERAAALAAEEVQHFRRVVAILRKRDWPL
                     GGRRRNPWAQSLRARIALGREPWTKVDRLLFGAMIEARSCERFTRLLEIIDDPEVAHL
                     LADLGPAERRHWQLFYHLASRSTDEKALEARFQGWKEFEAGLARGLGTSPAVHG"
     gene            complement(1342..>2165)
                     /locus_tag="VEL74_05740"
     CDS             complement(1342..>2165)
                     /locus_tag="VEL74_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62061.1"
                     /translation="DTINCGIPSAPELNGAVLACSSCHTIDPNANPGTAKPGFFGTSG
                     QSGFDFNPQLLKTPHLRNLYQKVGMFGNPVNPGFLGGDNGFKGDQVRGFGFLHDGSVD
                     TVFRFTHGLSFAEEFVGPGNNGILIGPEGEVQRRQLEAFILAFPTNLAPVVGQQITQT
                     AASSAAVGSRINLLRQRAEAGECDLVAKATVFGEEAGFLYKGAGLFESDRRGLPPIPA
                     AVLRSLATDLGRPVTYTCVPPGSGKRVGVDRDGDGVWDGDERFARTDPADPASRP"
BASE COUNT          354 a          731 c          757 g          323 t
ORIGIN      
        1 accgtgaaga tgtcctcgac gatcagccag ccgatcgcga tgcggccgtt gaccgtatcg
       61 aggatcccgc ggctctccaa cgcgcggagc agcacgaccg tgctcgccac cgagaggcag
      121 aggccgaagg cgatgcccgc cggcaggctc cagccccaga gcgtcgcgac gcccgcgccg
      181 agcgccgtgg cgacggcgct ctggacgatc gcgccgggca gcgcgatctt tcggacctcc
      241 agcaggtcgg agaacgagaa gtgcagccca acgccgaaca tgaggagcat gaccccgatt
      301 tcggcgagct ggctggcgag cccgacatcg gcgacgaagc ccggcgtcgc cggaccgatg
      361 acgatgccgg cgacgaggta gccgacgatc gccggcactt tcaggcggac ggcgatgaag
      421 cccaggatca gggcgagccc gaggccggcg gcgatggtgg tgatcagggg aacgctatgc
      481 ggcatgcagc tccttgtggg agaacggcgg gttgggcgtg ggcgccgagc ccggctcggc
      541 ctcgttcctg gccggccgat tctatacgcc ggggaggccg gtcgttctga ttagcgggtc
      601 aggctcagga ttgcggagtg cgtgcatcag acctgaaccg ggaccggcgt gaaccggtat
      661 gatcccggcc atgaggcgtg ccgacgtcaa cgagagggac tcgaccgagc cgatgggctt
      721 cgaggagatc tatgcggagc ttcctctcct ccacccgact cctcgctcct gggcggagct
      781 cgcggcggcg cggctgcccg agttcctggc cgatcacgcg gtctgcgagc agcaggtcgc
      841 catgtatgcc ctgtccctcg ccggctacta tcccgacgac gccgagctcg tcgagcgcgc
      901 ggcggctctc gcggccgagg aggtgcagca tttccggcgg gtggtggcga tcctgaggaa
      961 gcgggactgg ccgctcggcg ggcgccgaag gaacccctgg gcgcagagcc tgcgggcacg
     1021 gatcgctctc ggccgcgagc cctggaccaa ggtcgaccgt ctgctgttcg gcgccatgat
     1081 cgaggcgcgg agctgcgagc ggttcacccg tctcctcgag attatcgacg acccggaggt
     1141 cgctcacctt ctcgcggacc tgggccctgc agagcgccgc cactggcagc tcttctacca
     1201 tctcgccagc cgtagcaccg acgagaaagc cctggaagcg cgcttccagg gctggaaaga
     1261 gttcgaagcc ggcctcgccc gagggctcgg cacctctccc gccgttcacg gctgaggaga
     1321 ggtgccggac ctgtccccgg atcagggcct gctggccgga tccgccggat cagtacgagc
     1381 gaagcgctcg tcgccgtccc agacaccgtc tccgtcacgg tccacgccga cgcgcttccc
     1441 ggagccgggc ggcacgcagg tgtacgtcac gggccggccc agatccgtag ccagagatcg
     1501 cagcaccgcg gcggggatgg gcggcagccc acgccggtca ctctcgaaca ggcccgcccc
     1561 cttgtaaagg aagcccgcct cttcgccgaa gaccgtggcc ttggcgacga gatcgcactc
     1621 ccccgcctcc gcccgctggc gcaggaggtt gatgcgcgag cccaccgccg cggagctggc
     1681 ggcggtctgg gtgatctgct ggcccacgac gggcgccagg ttggtgggga acgccaggat
     1741 gaacgcctcg agctgacgcc gctggacctc cccctccggc cctatcagaa tgccattgtt
     1801 gccgggcccg acgaattcct ccgcgaagct gagcccatgg gtgaaccgga acactgtgtc
     1861 gacgctcccg tcgtggagga acccgaagcc gcgcacctga tcccccttga aaccgttgtc
     1921 gccgccgagg aagccggggt tcacggggtt cccgaacatt cccacctttt gatacaggtt
     1981 ccgcaggtgg ggggtcttca agagctgcgg attgaagtcg aagcccgact ggcccgacgt
     2041 gccgaaaaag ccgggtttcg cggtgccggg gttggcgttc ggatcgatcg tgtgacaact
     2101 gctgcacgcg agcacggcgc cgttgagctc cggagccgac ggaatgccgc agttgatggt
     2161 gtcga
//
LOCUS       DASPJI010000216         6254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_305023, whole genome shotgun sequence.
ACCESSION   DASPJI010000216 DASPJI010000000
VERSION     DASPJI010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305023"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>6254
                     /locus_tag="VEL74_05745"
     CDS             <1..>6254
                     /locus_tag="VEL74_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62062.1"
                     /translation="LLMLGPARYELLLSLPALCTDGAGLCLLVSEIGRCYAAVLAGRS
                     PGGEPTQYADASEWQNEVLEAEDTEEGRRFWQARDVFEGADLRLPFEVEIEASAPFRP
                     ESVSRVLDPEVAEAARELARRSGLPLATVLLGAWHLLLQRLSGEPEVGIGVAFDGRKF
                     EELREALGLFARYLPLRAPVGDGFEDLLGRLARSAAELGKRQEYFSWEILGETPVRPL
                     FPAWVFDLQELGTESPVPGLVLAIDRWSVHVDRFRLKLSCLHIADRLVAELWYDPAAL
                     RRTDVERLAGWYEALLRRALESPLAPLANLELLTEEERRRVVEDFNRSTADLGPPRTL
                     SSLFEEQAGRSPADVALVFRSRTMSYGELDDLSNRLAHRLRRLGVGPEVRVALCIERS
                     PELVAGILGIIKAGGAYVPLDPAYPRDRLALMLADSRAEVLVTRETLLEALPVTEARI
                     LCVDRDRESIDLESPLSLADGAVPENLAYVIYTSGSTGRPKGVMVSHRAILNRLLWTL
                     SVFPMGSGDVLLQKTAYSFDASIWEIFAPLFVGARLVLAEPGGQQDSAYLLEAISQEG
                     VTVIQLVPSQLAVFLDQRAVGEKCGTLRRLFCGGEALPGELVRRVHETIPGAALCNLY
                     GPTEAAIDATFHPCPVGGPEGALPVTPIGRPLANVQVYLADDCGLVPPGLPGELLIGG
                     VGLARGYLGRPDLTAERFVPNPWGAPGERLYRTGDRARHTPDGLVEFLGRMDAQVKIR
                     GFRIEPGEIEAALLRHPGLREAAVVAREDLPGDLRLVAYAVPAGGLADDRRLHRLPNG
                     LEVAYINRNETDVVYREVFEDESYLVHGIVLSDGACIFDVGANIGLFSLWVKDRFPKS
                     RVLAFEPLPPIFDTLRANVGLYGLDVRLFQLGLSDRGGTARFTFYPGWSAMSGRYADE
                     REEEEITRAILANQDLTGSADDLLAGRFRTQTFDCRLATLSEVIREQGIERIDLLKVD
                     VEKSELDVLQGLREEDWGKVGQVVIEVHDANGRLGQVRDLLESHGFAVTVDQDPVLHG
                     TGMYNVYAIHPERRPDGIAATRGARDGSALSRRAAPSPPGTRELREFLAERLPEHMIP
                     SSFVLLPALPRLANGKLDRRALPLPLPEIAGEGTATPRTPTEELVAELWKDLLKLDQV
                     GIDDDFFAMGGHSLLGTQLITRLRQVFAVDLPLRALFQAPTVAGLAETVEAARRGEEG
                     LLAPPLERVPREEGAELPLSFAQQRLWFLDQLEPGSPVYNLPLAVRLAGCLDRAALAV
                     TLREVVRRHEALRTRFLAVEGRPVQVIDMPPAEWLLPLVDLHGLPATAAEPEVTRLAR
                     EEVATPFDLERGRLLRTTLVRLGGEEHALLATMHHIVSDGWSTGVLVREIAGLYAALV
                     AGRPSPLPELPIQYADFAAWQRSWLAGEVLESQLDYWRQQLAGSQPVLDLPFDRPRPP
                     VPSFRGARHAFLIQEPEVLALLGRREGATLFMTSMAVFQALLHRYSGQDDVLVGTPVA
                     NRMRPELEALIGFFVNTLVLRADLSGNPSFRRHLAAVRESALGAWDHQGVPFENLVDA
                     LRPVREPSHNPVFQVMLALQNAPVEALELPDLELRPQQMGASTSKFDLTLELTEVPGG
                     IFGSIEYDTHLFDASTMARLARHFTRLIEEVGRDATRGIDEIEILDDAERRQVLLEWN
                     DTERPLPDSRLVHEIIADQAAESPNAKALVWSGGALTYADLVSLSGTLARHLRVMGIG
                     PEAPVAVCAERSPELIVAALAVLIAGGAYLPLDPSHPPERLRYTLEDAGNPLLLTQTR
                     LLPLFPQEARVLPLDGPAGAGEERGGAPGAGDWQRGWVGGGSAHEGAEQGDTAPLRVG
                     GDHLAYVIYTSGSTGRPKGTLLTHRGFRNMAGEMASALALGPGARLLQFSSPSFDASV
                     CEVFSTLTSGAELHLATLEEMLPGPDLIQLLRDRGISSVILPPSALRISPVEALPALG
                     TLMAAGEACAPELVTSWGASRRFLNGYGPTEATVCASIGLPERGDPRPAIGRNLANVR
                     LYVLSAAGQPAPTGVAGELFIAGMGLARGYLGRPELTAERFLPDPFSTLPGGRLYRTG
                     DQVRRRTDGQL"
BASE COUNT          836 a         2278 c         2091 g         1049 t
ORIGIN      
        1 ctcctgatgc tgggaccggc acgctatgag ctgctcctct ccctgcccgc tctctgcacc
       61 gatggggcgg gtctctgcct gctggtatcc gagatcgggc gctgctatgc cgcggtgctg
      121 gcgggacgat cgcctggtgg cgagccgacc caatacgccg acgcctcgga atggcagaac
      181 gaggtgctcg aggcggagga taccgaggag gggcgacggt tctggcaggc ccgcgacgtc
      241 ttcgagggag cggatctacg gcttcccttc gaggtggaga tcgaggcgtc tgcccctttt
      301 cggccggaga gcgtctctcg tgtcctcgat ccggaggtcg ccgaggccgc ccgggagctc
      361 gcccggaggt ccgggttgcc cctggcgacg gtcctgctcg gtgcctggca cctgctgctg
      421 cagcggctat ccggcgagcc ggaggtcggc atcggtgtcg ccttcgatgg acggaagttc
      481 gaggagctgc gggaggctct ggggctcttc gcgcgctacc tcccgctgcg ggcgccggtc
      541 ggcgacggct tcgaggatct gctcggcagg ctcgcccgct cggccgcgga gctcggcaaa
      601 cggcaggagt acttcagttg ggagatcttg ggagagacgc cggtccggcc gctgttcccc
      661 gcctgggttt tcgacctcca ggagctgggc accgagagtc ccgtccccgg cctggtgctc
      721 gccatcgacc gctggagcgt ccacgtcgat cgtttccgtc tgaagctttc ctgcctccac
      781 atcgcggacc ggctcgtggc ggagctctgg tatgaccccg cggccctgcg tcggaccgat
      841 gtcgagcggc tcgccggctg gtacgaggcg ctgcttcgcc gcgccttgga gagccccctg
      901 gctcctctgg ccaacctcga gctcttgacc gaagaggagc ggcgccgggt cgtcgaggac
      961 ttcaaccgca gcaccgccga tctcgggcca ccccgcacgc tctcttcgct tttcgaggag
     1021 caggccgggc gctcgccggc ggacgtcgcc ctcgtcttcc ggagtcggac gatgtcctac
     1081 ggcgagttgg acgacctgtc caaccgcttg gcccatcgcc tccggcggct gggtgtcggg
     1141 cccgaggtgc gggtggccct ctgcatcgag cgctcacccg agttggtggc cggcattctg
     1201 ggcatcatca aggccggcgg cgcctatgtt cctctggatc cggcctatcc gcgagatcgc
     1261 ctggctctga tgctcgccga ctcgcgggcg gaggtcctgg tcacccggga aacgctcctc
     1321 gaagctctcc cggtcaccga ggcacgcatc ctctgcgtgg accgggatcg cgagtcgatc
     1381 gacctcgaga gcccgctctc cctcgcggac ggcgcagtgc cggaaaacct ggcctatgtc
     1441 atctatacct ccggctccac cggtcgtccc aagggcgtga tggtgtccca ccgggcgatc
     1501 ctgaaccgcc tgctctggac gctgagcgtc ttccccatgg gctccggcga cgtcctcctc
     1561 cagaagacgg cgtacagctt cgatgcctcg atctgggaga tcttcgcgcc cctattcgtc
     1621 ggtgcgcggc tggtgctggc cgagcccgga gggcagcagg acagcgccta cctcctggag
     1681 gccatctccc aggagggggt taccgttatt cagctcgtac cctcccaact cgccgtcttc
     1741 ctcgaccagc gggcggtcgg ggagaagtgc ggcacccttc ggcggctctt ctgcggtggc
     1801 gaggccctcc cgggtgagtt ggtgcgacgg gtgcacgaga ccatccccgg cgccgcgctc
     1861 tgcaacctgt acgggcctac cgaggccgct atcgacgcta ccttccaccc ttgcccggtg
     1921 ggcggcccgg agggcgcgct cccggtgacg cccatcgggc gccccctggc gaacgtccag
     1981 gtctacctgg cggacgattg cggcctggtc cccccaggcc tgcccggtga gctgctcatc
     2041 ggcggcgtcg gcctcgcccg cggctatctc ggccggcccg acctgaccgc cgagcgcttc
     2101 gtccccaatc catggggggc gcccggcgag cgcctctacc ggactggcga ccgcgcgcgc
     2161 catacgccgg acgggctggt cgagttcctg gggcgcatgg acgcccaggt caagatccgc
     2221 ggcttccgca tcgagcccgg agagatcgag gccgccctgc tgcgccaccc tggcctgcgc
     2281 gaagcggcgg tggtggcgcg cgaggacctg ccgggcgatc tccggctggt cgcctacgct
     2341 gtgcccgcgg gcgggctcgc ggacgaccga cgcctgcacc gtctgccgaa tggtctcgaa
     2401 gtggcctaca tcaaccgcaa cgagaccgac gtcgtctacc gtgaggtgtt cgaggacgag
     2461 agctatctcg tccatggcat tgtcctctcc gatggcgcct gcatcttcga cgtgggagcc
     2521 aacatcgggc tcttcagcct ctgggtcaag gaccggttcc ccaagagccg ggtcctcgcc
     2581 ttcgagccgc tcccgcccat cttcgacact ctgcgtgcca acgtcggtct ctacggtctc
     2641 gacgtgaggc tgttccaact cgggctctcc gaccgcgggg gcaccgcccg gttcaccttc
     2701 tatcccggat ggtcggccat gtccggccgc tatgccgacg agcgcgagga ggaggagatc
     2761 actcgcgcca tcctcgccaa ccaggacctc accggcagcg ccgacgacct gctggccggc
     2821 cggttccgca cccaaacctt cgactgccgc ctcgccacgc tctcggaggt gatccgggag
     2881 caggggatcg aacgaatcga cctgctcaag gtggatgtcg agaagagcga gctggacgtg
     2941 ctccaggggc tccgggagga ggattggggc aaggtgggac aggtcgtcat cgaggtccac
     3001 gacgcgaacg gacggttggg ccaggtgcgc gatctactgg agagtcacgg cttcgcggtc
     3061 accgtcgacc aggacccggt gctgcacggc accgggatgt acaacgtcta tgcgattcat
     3121 ccggaacgcc ggccggatgg catcgcagca acgcggggag cccgggacgg ctccgctctc
     3181 tcccgccggg ccgctccctc ccctcctggg acgcgggagc tgcgggagtt cctcgccgag
     3241 cggttgccgg agcacatgat cccgtcgtcg ttcgtgctgc tgccggcgct ccctcgattg
     3301 gccaacggca agctcgaccg ccgcgcgctt ccccttccct tgccggagat tgcgggggag
     3361 ggcacggcca ctcctcggac cccgaccgag gagctcgtgg cggagctgtg gaaagatcta
     3421 ctcaagctcg accaggtggg gatcgatgac gactttttcg ctatgggcgg gcactccctg
     3481 ctgggcaccc agctcatcac ccgcctgcgc caggtgttcg ccgtcgatct gcccctgcgg
     3541 gccctcttcc aggctcccac cgtggcgggc ctcgccgaga ccgtggaggc ggcgcgccgc
     3601 ggtgaagagg gtctcctggc gccgccgctg gagcgcgtgc cacgtgagga gggcgcggag
     3661 ctgcctctct ccttcgccca gcaacgcctc tggttcctcg accagctcga gccggggagc
     3721 cccgtctaca atctgcctct ggccgtgcgg ctggccggtt gcctcgaccg ggcggcgctc
     3781 gcggtcacct tgcgcgaggt cgtccgccgc cacgaagccc tgcgtacccg cttcctggca
     3841 gtggaggggc ggccggtcca ggtgatcgac atgccgccgg ctgagtggct cctgcccctg
     3901 gtcgatctgc acgggctccc cgccactgcc gcggagcccg aggtgacacg actagctcgc
     3961 gaggaggtag ccaccccgtt cgacttggag cgcggccgct tgctgcggac gaccctcgtg
     4021 cgcctcggcg gcgaggagca tgccctgctc gcgaccatgc atcacatcgt ctcggacggc
     4081 tggtccacag gcgtcctggt gcgcgagatc gccgggctct acgccgccct agtggccggg
     4141 cgcccgtcgc ctctccccga gttgccgatc cagtacgccg acttcgccgc ctggcagcgg
     4201 agttggctgg cgggagaggt gctggagtcc cagctcgact actggcgcca gcagctcgct
     4261 ggcagccagc cggtgctcga tctgccgttc gaccggccac ggcctcccgt gccgagcttc
     4321 cgcggtgccc gccacgcatt cctgatccag gagcccgagg tcctcgccct cctaggccgc
     4381 cgggaagggg cgacgctctt catgacttcg atggccgtct tccaggcgct cctgcaccgc
     4441 tacagcgggc aggatgacgt gctggtgggg acgccggtgg ccaaccgcat gcgccccgag
     4501 ctggaggcgc tgatcggatt cttcgtcaat accctggtgc tgcgggccga tctctcaggc
     4561 aacccctcct tccggcgaca cctggcagcc gtccgggagt cggccctggg ggcctgggac
     4621 caccagggcg tccccttcga gaatctcgtg gacgccctcc ggccggtgcg cgagccgagt
     4681 cacaaccctg tcttccaggt gatgctcgcc ttgcagaacg ccccggtgga ggccctggag
     4741 ctgccggatc tggagctgcg gccccaacag atgggggctt ccacctccaa gttcgatttg
     4801 accctggagc tgaccgaagt cccgggcggg atcttcggct ccatcgagta cgacacccac
     4861 ctcttcgacg cctccaccat ggcgcggctc gctcgtcact ttacccggct gatcgaggag
     4921 gtcggccgcg atgccacccg cggaatcgat gagatcgaga tccttgacga cgccgagcgc
     4981 cgtcaagtcc tcctggaatg gaacgacacc gagcgtccgc tgcccgattc ccgtttggtc
     5041 cacgagatca tcgccgacca ggcggcggag agccccaacg cgaaggccct cgtttggagc
     5101 ggcggcgcgc tcacctacgc ggatctggtg agcctttccg gtactctggc ccgccacctg
     5161 cgcgttatgg gcatcggacc cgaagccccg gtcgcggtct gcgccgagcg ctcccccgag
     5221 ctaatcgtgg cggccctcgc cgtgctcata gcggggggcg cctatctccc tctcgaccca
     5281 tcccatccac cggagcgcct gcgctacacc ctggaggacg ccggcaaccc gctcctcctc
     5341 acccagaccc gcctgctccc tctcttcccg caagaggccc gtgtcctccc cctggacgga
     5401 ccggccgggg cgggggagga gaggggcggt gcgcccggcg caggcgactg gcagcgtggg
     5461 tgggtgggag gggggagcgc acacgaagga gccgagcagg gcgacaccgc ccctctccgg
     5521 gtgggtgggg accacctcgc ctacgtcatc tacacctcgg gctccaccgg ccgtcccaag
     5581 gggacgctcc tcacgcatcg cggcttccgc aacatggccg gcgagatggc gagcgccctc
     5641 gcgctcggcc ccggcgcccg gctactccag ttctcgtccc cctctttcga cgcctcggtc
     5701 tgcgaggtct tcagcaccct cacctccggc gccgagctcc acctggcaac cctggaggag
     5761 atgctccccg gccccgacct gatccagctc ctgcgcgatc ggggaatcag ctccgtcatc
     5821 ctgccccctt cggcgttgcg catctccccc gtggaggctc tcccggctct cggcaccctg
     5881 atggcggcgg gggaggcctg cgcgcccgag ttggtgacct catggggggc ctcccgccgg
     5941 ttcctcaacg gctacggccc caccgaggcc acagtctgcg cctccatcgg cctgccggag
     6001 cggggggacc cgcgtccggc catcggccgc aatcttgcca acgtccgcct gtacgtcctg
     6061 agcgccgccg gacaaccggc ccccaccggc gtcgcgggcg agctgttcat cgccgggatg
     6121 ggactcgccc gtggctatct cggccggccg gagctgacgg cggagaggtt cctccccgat
     6181 ccgttctcca ctctcccggg aggccgcctc taccgtaccg gcgaccaggt acggcggcgg
     6241 accgacggcc aact
//
LOCUS       DASPJI010000217         6787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_474764, whole genome shotgun sequence.
ACCESSION   DASPJI010000217 DASPJI010000000
VERSION     DASPJI010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6787)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6787
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474764"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3779
                     /locus_tag="VEL74_05750"
     CDS             <1..3779
                     /locus_tag="VEL74_05750"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HYG62063.1"
                     /translation="VERLVDEVVGMPGGLPLLSYALSQLYFAYVDAGRGDRLLTAADL
                     AAIGGDATASEGPVAAGGIVRILRKAADGVVQALPDGPHRETLWRVLLRMVNLVGVRP
                     TRRRVPSWELVYPDPAENRRRKAVLAELIGEKARLLVSGPDPEAGKAIRVAEEEEEPD
                     AIIEPAHDELLVAWPELARRIEAARVLLPIHRRLGEAAAEWWAGGCRTRDLDLRLLGL
                     VETGDLPQDWLNKVEDRYVTAAREERRWRTRRRWTAAVVAFLAISGAALFFYDQSQRN
                     ARLSYQSRIQGLVNQAGADLDAGRTARGLLLARQAFGFLDRSRGHWASRMGLSLPWIS
                     SRDEVDLEARIDQVFRRVMDDLPPSFVVLRDPWSSEPVAFHPGGRLLVTVHRSTPVLV
                     TLPEKGVQPEGPVQPLAGVPAGAALQTIAFSADGRFLAVGFFGGALCVWRLDGTPPAP
                     VPGTPVCAAGRGAVTGLAFRPGTSALVAGRDDGTGAGSLQVWEVAGGQGRPPRLTRGF
                     ERPLPEGVQSLAVSPSGDLFLSLKRTAGVLRGTLDPQAALAPLEAPADQEVESLTLDD
                     SGRLLAGLVTPGGEILGWELPEGRPFSLNRQGTAGAMALAQAGDHLVTVDNDERLSRI
                     GGELPAEPEEVRIPSLCDSGPVEVAVPRGGQRIAVFCDGALRLWEPGPMEGDASRGIG
                     RTHVSVFGMPTAFAALPGSATPLAIADANGDVELHDLKTGQKLHIPHPSSTGMLGLDL
                     TLSPGLGSSFGGSGITGVAWGPGGTVLHVLAQDGLWWTYDVLTRTVGTRRLGGRVCAA
                     AGSADGVWLAVLFDPLSSAPILALYKSDGAPVSLPPPPSARGASSLSGAGLPVFPISR
                     YTVAGRCKAGLAFHPDRTELAVGIGNAVSLYDVPADGRWRSRRIAETRGMIFAAAWKG
                     DLLAIGGGVAGATSDGFVELLGMDGGVRYTWTVPGAAVSGLAFEPGGGRLAASTDGGG
                     VYVWTDGASGPSAILGKSGDFTEFPQFLEPGRVAALTSGGASVWSLGTRELADTFCAT
                     AASNLESRDWVNYVDADLGRYEPACPALPFHPSLLGQADSLAAAGRGEEAQRLYRWIN
                     ELEPGSVVNPARRARGQALAAELSGFDAMDRQGVLAGLRRAEEHEELRRAEDLLPLPK
                     DALIRLCRRSVLFGEARRALPFCEDGVKLHGDVGAARDSRGLALAVLGQLVRAREDFL
                     ASRDWLEEPSWRERRQAWIVALGAGRNPITPQVLCDIAREELKVDPSAPPAAPN"
     gene            3827..4162
                     /locus_tag="VEL74_05755"
     CDS             3827..4162
                     /locus_tag="VEL74_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62064.1"
                     /translation="MPGKLFRFLIAASLVFCALFVSTAAGSAQTGLVRPGPHGAPHGA
                     LFLSYTGACGGWVRDADNDVYCCDLDERPTCDDEGSCECSDDDYCEKLCKPAGTCDQR
                     GPVCPEEEE"
     gene            4216..4872
                     /locus_tag="VEL74_05760"
     CDS             4216..4872
                     /locus_tag="VEL74_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62065.1"
                     /translation="MNGKLVRFLPLLTLLMGSPAWPQAPLQPIPRSEGRFCNACVRPQ
                     VPLRVELVWDGEVDLDLWVAWDRFVAYYDDKTGGSATGRLDCDVQKGGRYESDPACRG
                     ETENFYATSAPAAPGRLLYRFAVHHYDGEGGDPVPWRARVTLFETRHFFCSGVFDGEA
                     AAGDVGMADYLKAGSRSGRLLGFSPVFEIVLPSPGAGAVPVANCEATVPVSWAEGGRR
                     "
     gene            4869..>6787
                     /locus_tag="VEL74_05765"
     CDS             4869..>6787
                     /locus_tag="VEL74_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62066.1"
                     /translation="MSRPGSVRRRLFLGVSAIAISLATTGPLTAGQCVKAEFLDHLET
                     SGFSLEALDTIERFALEKTVCPWDEGKDRFCPSPGRYVVWRNNLESYWFPVPDAPSAD
                     RYLAVALRITARWKELNPAGRYDEAHAFSRVVLQGNCFPLGLSSGRGLLDQMEALKQR
                     REQALQDQEALRELSRELADEIRKGEGVDSGTLQALHERIRQTADGIGKELLEGEGEG
                     LGELGETVANAGGDEEPGEKGGNSTNGGEGVLPGSAPEWLQRVAKAVAQYFGVDTALE
                     SAALLLLLLEPELFTELADLVGGLSRSLVPDDLSGFLDSATELYDAFAAATALYETIT
                     SPESQFKDLVSRTGELLKALPEEVRAELEKGAAGEALAFGERLGQIAGAIPAEEDLAL
                     VEKLFSEEGRDAAFQELEKKVKAKIETEAETALAKALVQGGAELGIDLPAADLAKGIF
                     AGEFDQVGERTLRAGLAKLEVGGVSLSVAEAEALAEGEAGPFVRGRLRKGLETALGRD
                     LPPDLTGALDQAAGLLEGGDVETALQLVERSLTEAGEGALAKSKEELRAALCPPGAGA
                     EACTLGDLPAAWRDRLVADARAVQIGNRRLFSEEEIAAIESGDLSGLAARARNRAEIQ
                     LEEIGLAPETARRVLR"
BASE COUNT          977 a         2276 c         2467 g         1067 t
ORIGIN      
        1 tggtcgagcg gctggtggac gaggtggtgg gcatgccggg agggctgccg ctcctctcct
       61 acgccctgag ccagctctac ttcgcctatg tggacgccgg acgtggcgat cggctgctca
      121 ccgccgccga cctcgccgcc atcgggggag acgctacggc gtccgaaggg ccggtcgccg
      181 cgggcgggat cgtccggatc ctgcgcaagg cggcggacgg ggtggtccag gcgctaccag
      241 acggccccca tcgagagact ctctggcggg tcctcttgcg catggtgaac ctggtggggg
      301 tccggccgac gcggcgccgg gtgccgtcct gggagctggt ctatccagac ccggccgaga
      361 accggcgccg gaaggcggtg ctcgcggagc tgatcggcga aaaggcgcgc ctgctggtga
      421 gcgggcccga cccggaggcg ggcaaggcga ttcgagtagc cgaagaggag gaagagccgg
      481 acgcgatcat cgagccggcc catgacgagc tgctggtggc ctggccggag ctggcgcggc
      541 gcatcgaggc agcccgcgtc ctgctcccga tccaccgccg cctcggcgaa gccgccgcgg
      601 agtggtgggc cggcggctgc cgcacccgag acctcgacct ccggctcctc ggcctggtcg
      661 aaaccggcga cctgccacag gactggctca acaaggtcga agacaggtac gtcaccgccg
      721 cccgcgagga acggcgctgg cggacccggc ggcgctggac cgccgctgtg gtcgccttcc
      781 tcgcgatctc cggcgccgcg ctcttcttct acgaccagag ccagcgcaac gcgcgcttga
      841 gctatcaatc caggatccag ggcctggtca accaggcggg ggccgatctc gatgcgggcc
      901 ggacggccag aggactgctc ctggcccgcc aggccttcgg ctttctagat cgcagcaggg
      961 ggcattgggc ctcccgtatg ggcctctccc ttccctggat ctcctcgcgc gacgaggtcg
     1021 acctcgaagc ccgtatcgac caggtcttcc gtcgggtgat ggatgatctg ccaccgagct
     1081 tcgtggtgct gagggaccct tggagctctg agccagtcgc cttccatccg ggcgggcggc
     1141 tcctggtgac cgttcatcgg tcgactccag ttctcgtgac cctgccggag aagggggtcc
     1201 agccggaggg gccggtccag ccgctggccg gcgtgccggc cggggctgcg ctccagacga
     1261 tcgccttcag cgcggacggg cgctttctgg cggtgggctt cttcggtggt gctctttgcg
     1321 tctggcgact cgacgggacg ccgccggcgc ccgtgccggg aacgcccgtt tgtgcggcgg
     1381 gccgcggtgc cgtgaccggt ctcgcgttcc gcccaggcac ttcggctctc gtcgcggggc
     1441 gcgacgatgg aacgggtgcc gggagcctcc aggtctggga agtcgctggc ggtcagggga
     1501 gaccaccccg actgacgcgc ggctttgagc ggccattgcc ggaaggtgtc cagagcctgg
     1561 cggtctcgcc gtcgggcgat ctattcctct ccctcaagag gacggccggt gtcctgcgtg
     1621 gaacgctgga cccgcaggcg gctcttgcac ccctggaagc gcccgccgat caggaggtcg
     1681 agtcgctcac tctcgacgac tccggacggc tcttggccgg gctcgtgacg ccggggggcg
     1741 agatcctggg gtgggagctt cctgaggggc ggccgttctc gctgaaccgg cagggtaccg
     1801 ccggcgccat ggccctagcg caggccggcg atcacttggt gacggtggac aacgacgaac
     1861 ggctcagccg gatcggcggc gagctgccgg cggaaccgga agaggtccgt atcccttcgc
     1921 tctgcgacag cggtccggtc gaagtcgcgg tccctcgcgg tgggcagagg atcgcggtct
     1981 tctgcgacgg cgccctgaga ctgtgggagc ccgggccgat ggagggggac gcgagccgcg
     2041 gcatcgggcg aacgcatgtc tctgttttcg ggatgcctac ggccttcgcg gctctgccgg
     2101 ggagcgccac ccccttggcc atcgccgacg ccaacggcga tgtcgagctg cacgatctca
     2161 aaaccgggca gaagctccac ataccgcacc cctcctcgac cggaatgctc ggcttggacc
     2221 tcaccctgtc tccagggctg ggctcctcct tcggcggctc gggaatcacc ggtgttgcct
     2281 ggggcccggg gggcaccgtg ctgcatgtgc ttgcccagga tggcctctgg tggacgtacg
     2341 acgtgctgac caggacggtc ggcacacgcc gcttgggcgg ccgcgtctgc gcggccgctg
     2401 ggagcgccga tggggtgtgg ctggcagtcc tcttcgaccc gctctcgagt gccccgattc
     2461 tcgcgctcta taagagtgac ggagctcccg tgtctctgcc accgccgcct tctgctcgcg
     2521 gggcttcgtc tctttcagga gcaggcctcc cggtcttccc gatctcccga tataccgtcg
     2581 ccggccggtg caaagccggg ctggcgtttc atccggaccg caccgagctg gcggtcggga
     2641 tcgggaacgc agtcagcctc tatgacgtgc cggcagacgg acggtggcgc tcacggcgga
     2701 tcgccgagac ccgcgggatg atcttcgccg ccgcctggaa gggtgatctc ctggccatcg
     2761 gaggcggagt tgcgggggcc acgtcggatg gattcgtcga gctgctcggc atggacggtg
     2821 gggtgcggta cacctggacc gtgcctggag ccgcggtcag cggcctggcg ttcgagccgg
     2881 gaggagggcg actcgccgcc agtaccgatg gtgggggtgt ttacgtctgg acggatggcg
     2941 cttccgggcc atccgcgatt ctggggaagt ccggcgattt taccgagttc ccgcagttcc
     3001 tcgagcccgg ccgggtcgcc gcgctcacct cgggaggggc gtcggtctgg tctctgggaa
     3061 cccgggagct ggccgacact ttctgcgcca ccgccgcctc aaatctggag agccgggact
     3121 gggtgaacta cgtggacgcg gatctgggcc gctacgagcc ggcttgtccc gctctcccct
     3181 tccaccccag cctgctcggc caggccgact ccctggcggc tgccggcagg ggagaggagg
     3241 cacagcggct ctatcgatgg atcaacgagc tcgagccggg atcggtggtg aacccggccc
     3301 ggcgggcgcg tggccaggcg ttggccgccg agctctccgg cttcgacgcc atggaccggc
     3361 aaggtgttct tgccggattg cggcgggcgg aggagcacga agagctacgc cgggcggagg
     3421 acctccttcc cctgcccaag gatgccttga tccggctctg ccggcggagc gtgcttttcg
     3481 gcgaggcccg gcgagctctc cctttctgcg aagacggggt caagctgcac ggggacgttg
     3541 gcgccgcccg cgacagccgg ggtctcgcgc tggccgtcct cggtcaactg gtccgggccc
     3601 gtgaggattt cctcgcgtcg cgggactggc tcgaagaacc gtcctggagg gaacggcggc
     3661 aggcctggat cgtcgccctg ggagcaggtc gtaaccccat cacaccgcaa gtcctttgcg
     3721 atatcgcccg cgaggagctg aaggtcgatc cgagcgctcc gccggccgcc cccaactagg
     3781 ggaaatcact cctcgcgtcc gtctatcggg gcaaggagga aacacgatgc ccggcaagct
     3841 tttccgtttc ttgatcgctg cgtcactcgt cttctgcgcc ctcttcgtga gcacggccgc
     3901 gggctccgcc cagaccggcc tggtccgccc cggtccccac ggcgctcccc atggggcgct
     3961 cttcctgtcg tacacgggcg cctgtggcgg ctgggtccgg gatgccgaca acgacgtcta
     4021 ctgctgcgat ctcgatgagc gccccacctg cgacgacgaa ggatcgtgcg agtgctccga
     4081 cgacgactac tgcgagaagc tgtgcaagcc ggcggggacc tgcgaccaga ggggacctgt
     4141 gtgtcctgaa gaggaggagt gaacccgcac agtcgccaga aaccaccgcg cctcccccgc
     4201 tccgagatcc ggcacatgaa cggcaagctc gtcagattcc tcccactcct gactctcctg
     4261 atgggctcgc cggcatggcc gcaggcgccg ctgcaaccca tcccccggtc cgaaggccgg
     4321 ttctgcaacg cctgcgtccg gccccaggtg ccgttgcgcg tcgagctggt ctgggacggc
     4381 gaggtggacc tcgacctctg ggtggcctgg gaccgcttcg tcgcctacta cgatgacaag
     4441 accggcggct cggccaccgg ccgcctcgac tgtgacgtcc agaagggggg ccggtacgaa
     4501 agcgatccgg cgtgccgcgg cgagaccgag aacttctacg ccaccagcgc cccggcagcc
     4561 ccgggccggc tgctctaccg gttcgccgtc catcactacg acggtgaggg aggcgatccg
     4621 gttccctggc gggcgcgggt cactctcttc gagacccgcc acttcttctg ctcgggggtg
     4681 ttcgacggcg aggccgccgc gggagatgtc ggaatggccg actatttgaa ggcgggctcg
     4741 cggagcgggc gcctgctcgg tttctctccg gtcttcgaga tcgtcctccc ctcgcccggg
     4801 gccggggcgg taccagtggc gaactgtgag gcgacggtac cggtaagttg ggccgagggg
     4861 ggacggcggt gagccggccg ggatctgtcc ggcggcgcct cttcctgggg gtgagcgcca
     4921 tcgccatctc cctggccacc accggtcctc ttaccgcggg gcagtgcgtc aaggcggagt
     4981 tcctcgacca tctcgagacg agcggcttct ccctggaggc tctggatacc atcgagcgct
     5041 tcgccttgga gaagaccgtc tgcccgtggg acgagggaaa ggacaggttc tgcccctccc
     5101 cggggcggta cgtcgtctgg cggaacaacc tggagtccta ctggttcccg gtgccggacg
     5161 ccccgtcggc cgaccgctac ctcgcggtgg cactgcggat caccgcccgg tggaaggagc
     5221 tcaaccccgc aggccgctat gacgaggccc atgccttctc ccgggtggtg ctgcagggga
     5281 actgtttccc gttgggcctc tcctcgggcc gagggctcct cgaccagatg gaggcgctca
     5341 agcagcggcg cgaacaggcg ctccaagacc aggaggcgct gagggagctc agtcgcgagc
     5401 tggcggacga gatccgcaag ggcgaggggg tcgactccgg taccctgcaa gctctccatg
     5461 agcggatccg ccagaccgcc gacgggatcg ggaaggagct cctcgagggc gagggggagg
     5521 gactcggcga gctcggcgag accgtcgcca acgccggggg agacgaggag ccaggcgaaa
     5581 aagggggcaa cagcaccaac gggggtgaag gcgtcctgcc ggggagcgct cccgagtggc
     5641 tccagcgcgt cgccaaggcg gtggcgcagt acttcggggt cgacaccgcc ctggagagcg
     5701 cggctctgct cctcctgctc ctcgaacccg agctcttcac cgagctggct gacctggtgg
     5761 gaggcctgag ccgcagcctc gtgcccgacg atctcagcgg tttcctcgac tcggccaccg
     5821 agctctacga cgccttcgcc gccgccacgg ccctctacga gaccatcacc tcaccggaga
     5881 gccagttcaa ggacctggtg agccgcaccg gcgagctgct gaaagcactg ccggaggagg
     5941 tgcgcgccga gctggagaag ggggccgccg gcgaggcgtt ggcattcggc gagcggctgg
     6001 ggcagatcgc gggggcaata cccgcggagg aggacctcgc cctggtggaa aagctgttct
     6061 cggaggaagg cagggacgcg gcctttcagg agctggagaa gaaggtgaag gccaagatcg
     6121 aaaccgaggc cgagacggcg cttgccaagg ccctcgtcca ggggggcgcc gagctgggca
     6181 tcgacctgcc ggccgcggac ctggccaagg gcatcttcgc gggtgaattc gaccaggtcg
     6241 gcgagcggac cttgcgcgcg gggctggcga agctcgaggt gggtggagtg tcgctgtcgg
     6301 tggccgaagc cgaggccctg gccgagggcg aggccggacc ttttgtccgc gggcggctgc
     6361 gcaaggggct ggagaccgcc ctgggccggg acctgccgcc ggacctcacc ggggccctgg
     6421 accaagccgc cggactgttg gagggcggcg acgtggagac cgccctccaa ctggtcgaac
     6481 gatccctgac cgaagccggt gagggcgccc tggcaaagtc caaggaggag ctgagggccg
     6541 ccctctgccc gccgggtgcc ggtgccgagg cctgcactct gggggacctg ccggccgcct
     6601 ggcgcgaccg gctcgttgcc gacgcccgcg ccgtgcagat cggcaaccgc cgtctgttca
     6661 gtgaggaaga gatcgccgcc atcgagagcg gcgacctgtc aggtttggca gcccgggccc
     6721 gcaaccgagc cgaaattcag ttggaagaga tcgggctggc gcccgagacc gcccggcggg
     6781 tgctgcg
//
LOCUS       DASPJI010000218         2314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_474873, whole genome shotgun sequence.
ACCESSION   DASPJI010000218 DASPJI010000000
VERSION     DASPJI010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474873"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2314
                     /locus_tag="VEL74_05770"
     CDS             <1..>2314
                     /locus_tag="VEL74_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG62067.1"
                     /translation="VIAMDGFLQDLRFAFRALRQRPLPAVVAVVTLALGIGANAALFS
                     VVDAVLLSPLPFRDPERLAVVWASNPEVAAQVGLPDKLPVSPGAFYDWKRESRSFEKM
                     AMVSSDRVSLTGRGEPELLGAVAVTGEFFQVLGARPLLGRTLLPEDDELGERATALLS
                     HGLWRRRFGGDPEIIGQKIVLDGAPVTVVGVMPAGFAFPRAAELPNLYGFSREPDLWV
                     PMAMPLERRQSRGNRGQLAIGLLRPGVTIEAARQEMLPIGERMKKAYVEDTGWGVRPE
                     PLAEQLVGDVRPALLILLGAVGLVLLIACANVANLFLAQAVTREREIAVRTALGAGRG
                     RLARQLLTESLVTAAVGGALGLLAAYWGLRLFASWIPESVPVPAGLSLDGSVLLFTLG
                     AVFLAGLLSGLVPALQTTRTDLSAALREGGRGASGSAAGRRTRSLLVVAEVALAVLLV
                     IGAGLLIRSFVRLTGVDPGFRAERVLTFEVILPGSQYKRAQRAPFFAAVLERLRGLPG
                     VEAAGAVNNLPLSGEENIETITVEGQPPREPDDPALTDLRTAMPGYFETMGIALVAGR
                     TLEARDVAGGQQVAVITEGMAKAYFPGAGTGDAIGRRFHLGFTPDPEDENPWWTVIGV
                     VRDVRHTGLHSGPRPQMYLPETQFPMGQMTLVARTTGEPLALADEARAAVYSVDRDQP
                     ISKVRTMEQVLGRSVAGQRFNMILLTVFAGLALALAAVGIYGITAYSVTQRTREMGLR
                     LALGARRGQVLGLVVRQAGMLALAGIAAGLG"
BASE COUNT          268 a          845 c          854 g          347 t
ORIGIN      
        1 agtgatcgct atggacggct tcctccagga cctccggttc gccttccgcg ccctccgcca
       61 gcggccgctg ccggccgtgg tggcggtggt cacgctggcg ctggggatcg gcgccaacgc
      121 cgcccttttc agcgtcgtcg acgcggtgtt gctgtcgccg ctgccgttcc gcgacccgga
      181 gcggctggcg gtggtctggg cgtccaatcc agaggtcgcc gcccaggtgg ggctgccgga
      241 caagctgccg gtctcccccg gcgccttcta cgattggaag cgggaatcgc ggtcgttcga
      301 gaagatggcc atggtgagct ccgaccgggt gtccctcacc ggccgcgggg agccggagct
      361 gctgggcgcg gtggctgtca ccggcgagtt ctttcaggtg ctgggggccc ggccgctcct
      421 cgggcggact ctgctgccgg aagacgacga gctgggggag cgggccaccg ccctcctctc
      481 tcacgggctc tggcggcggc gtttcggcgg cgatcccgaa atcatcggcc agaagatcgt
      541 cctggacggc gcgcccgtca ccgtggtggg cgtcatgccc gcgggcttcg ccttcccgcg
      601 cgcggccgag ctccccaacc tctacggctt ctcccgcgag ccggacctct gggtcccgat
      661 ggccatgccg ctggagcggc ggcagagccg gggcaaccgc ggccagctcg ccatcgggct
      721 cctccggccg ggggtgacca tcgaggccgc gcgccaggag atgctgccga tcggcgagcg
      781 gatgaagaaa gcctacgtgg aggacaccgg ctggggggtg cggcccgagc ccctggccga
      841 gcagctcgtg ggcgacgtgc ggccggcgct cctcatcctc ctgggcgccg tcgggctggt
      901 gctgctgatc gcctgcgcca acgtcgccaa cctcttcctc gcccaggccg tgacccgcga
      961 gcgggagatc gcggtccgca ccgccttggg agcgggaagg gggaggctgg cgcggcagct
     1021 cctcaccgag agcctggtga ccgcggccgt ggggggcgcc ctgggcctcc tcgccgcata
     1081 ctggggcctg cgcctcttcg cctcctggat cccggagagc gtgccggtgc cggccggcct
     1141 ctcgctcgac ggcagcgtcc tgctcttcac cctgggtgcg gtcttcctgg caggcctcct
     1201 gtccggcttg gtgccggccc tccagaccac ccggaccgac ctctcggcgg cgttgcgcga
     1261 agggggccgc ggcgcctcgg gctccgcggc cggccgccgc acgcggagcc tcctggtggt
     1321 ggccgaggtg gcgctcgccg tgctcctggt gatcggcgcc ggcctgctga tccgcagctt
     1381 cgtccgcctg accggcgtcg accccggctt ccgtgccgag cgggtcctta ccttcgaggt
     1441 catcctgcca ggcagccagt acaagcgcgc ccagcgggcg ccgttcttcg ccgcggtgct
     1501 ggagcggctg cgcggcctgc cgggggtgga ggccgccggc gcggtcaaca acctgccgct
     1561 gtccggcgag gagaacatcg agaccatcac cgtggaaggg cagccgccgc gcgagcccga
     1621 cgacccggcc ctcaccgatc tgcgcaccgc catgccgggc tacttcgaaa ccatggggat
     1681 cgctctcgtc gccggccgga ccctcgaggc gcgcgacgtg gccggcggcc agcaggtggc
     1741 ggtgatcacc gaggggatgg cgaaggccta cttccccggc gccggcaccg gcgacgccat
     1801 cggccggcgc ttccacctcg gcttcacccc cgacccggag gacgagaacc cctggtggac
     1861 cgtgatcggc gtggtgcgcg acgtccgcca caccggcctg cacagcggtc cccggccgca
     1921 aatgtacctg cccgagacgc agttccccat gggccagatg accctggtgg cgcgcaccac
     1981 cggtgagccg ctggctctgg ccgacgaggc gcgggcggcc gtctactcgg tagaccgtga
     2041 ccagccgatc tccaaagtcc gcaccatgga gcaggtgctg ggcaggtcgg tggcggggca
     2101 gcggttcaac atgatcctgc tgaccgtctt cgccggcctc gccctggcgc tggcggcggt
     2161 cggcatctac ggcatcaccg cgtactcggt gacgcagcgg acccgggaga tggggctgcg
     2221 cctggccctc ggagcgcggc gcggccaggt gctgggtctg gtggtgcggc aggccgggat
     2281 gctggcgctc gccggcatcg ccgcgggcct cggc
//
LOCUS       DASPJI010000219         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_698631, whole genome shotgun sequence.
ACCESSION   DASPJI010000219 DASPJI010000000
VERSION     DASPJI010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698631"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3341
                     /locus_tag="VEL74_05775"
     CDS             <1..>3341
                     /locus_tag="VEL74_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62068.1"
                     /translation="FVRESGARLYRTGDLARRLVSGEVEFLGRADHQVKVRGFRIELG
                     EIEAALAAQPAVAQAVIVALPDTMCSTRLVAYLVPAEGDVADAEELRAALRESLPEYM
                     IPAHFIPLPALPLTPNGKVDRKALPAPGGERPDLRAEHLAPRTPVEEVLAAVWTEVLG
                     RPGIGVHDNFFKLGGHSLLATQVVSRIRQAFGVELPIRDLFEAPTVAGLAARVDRSGG
                     QAEVPPEVPPIKRVPREAHLPLSFAQQRLWFLERLSPGGVAYNIPLAVRLSGALDAQV
                     LENALLEVARRHEALRTTFAERDDLPVQVITPEPPIFLVVGDLSDLPQAARELEMSRR
                     ALAEALRPFDLAAGPLVRALLLRLDEEEHALLVTMHHIVTDGWSMGIFLRELTALYGA
                     FVQGRPSPLPELPIQYGDFAAWQRAWLTGGVLDAQLGYWQGRLAGAPPRLILPSDRPR
                     PALETHRGASRPVELTPELSHALATLAGREGATPFMVLVAGFAALLGRYSGQDDVVVG
                     SPIAGRTRREVEGLIGFFVNTLALRVELADRPDFSGLLRQVRRTALDAYAHQDLPFER
                     LVEELVTERDLGHSPLFQVMLAVQNTPAGPSGMPPVPGLELRPVEVEAGTAKFELSLS
                     FAPRGEELLGDLDYNTDLFDSATAERLARHFVRLLEGAVAAPEIPLPELSLLAPSEQQ
                     HVLREWNDTATDYGAESGVCLHGLVRAQVERAPDAVAAVSEDEHLSYRELARQAGCLA
                     PRLTEVGAGPESVVAICAERSLEMIVGMLGILEAGGAYLPLDPSYPRERLALILESSA
                     AAAVLVQDHLQDRLPAAPVPRLPLGPGREGLAWMPPEARPENLAYVIFTSGSTGTPKG
                     AMVPHRAIVNRLLWMQAAYGLSPADRVLQKTPFGFDVSVWEFFWPLLVGAPLVFARPE
                     GHKDPGYLAALIAEQGITVLHFVPSMLRAFLETDRQESCVSVRAVMASGEALPLDLTE
                     RFFEWMPGELHNLYGPTEAAVDVSSWACAPAEERRSVPIGRPIANLRLHVTDRSWQPA
                     PMGVAGELLIGGVGLGRGYLGRPELTAERFVPDPFAALWSEPGARVYRTGDLARQLPG
                     GEIDFLGRIDFQVKIRGFRIELGEI"
BASE COUNT          414 a         1129 c         1254 g          544 t
ORIGIN      
        1 ttcgtccggg agagtggagc gcgcctctac cgtacgggag acctcgcccg ccggctggtg
       61 agcggtgagg tggagttcct cggccgcgcc gaccaccagg ttaaggtccg ggggttccgg
      121 atcgagctgg gggagatcga ggcggcgctc gccgcccagc ctgcggtggc tcaggcggtg
      181 atcgtggccc tgccggacac catgtgcagt acccggctcg tggcttacct ggtccccgcc
      241 gagggggacg tggcggatgc ggaggagctc cgggcggctc tgcgcgagtc gcttccggag
      301 tacatgattc ctgcccattt catcccgctc ccggccctcc ccctgacccc caacggaaag
      361 gtggaccgga aggccctgcc ggcacccggt ggagagcggc cagatctccg agcggagcac
      421 ttggctcccc gcacaccggt cgaggaggtg ctcgcggcgg tctggaccga agtcctgggc
      481 cggcccggca tcggcgttca tgacaacttc ttcaagctgg gcggccactc gctgctggcg
      541 acccaggtgg tctcgcgaat ccgccaggct ttcggcgtgg agctgccgat ccgcgacctg
      601 ttcgaggcgc cgacggtggc cgggctggcg gcgcgggtcg accggagcgg cggccaggcg
      661 gaggtcccgc cggaggtccc gccgatcaag cgggtgccgc gggaggccca cctgccgctc
      721 tccttcgccc agcagcggct ctggttcctg gagcggctct cgccgggcgg cgtggcctac
      781 aacatcccct tggccgtgcg gctctcgggc gcgctcgacg ctcaagtgct cgagaacgcc
      841 ctgctcgagg tggcgcgccg ccacgaggct ctccgcacga ccttcgccga gcgcgacgac
      901 cttcctgtgc aggtcatcac gcccgagccg ccgatcttcc tggtggtcgg cgacctgtcg
      961 gatctccccc aggccgcgcg tgagctcgag atgtcgcggc gggcgctggc cgaggcgctg
     1021 cggccgttcg acttggcggc gggccccctc gtgagggcgc tgctgctgcg tctggacgag
     1081 gaggagcacg cgctgctggt gaccatgcac cacatcgtca ccgacggctg gtcgatgggc
     1141 atcttcctgc gcgagctcac cgcgctctac ggcgccttcg ttcagggccg gccttctccc
     1201 ctgccggagc tgccgatcca gtacggggac ttcgcggcct ggcagcgggc ctggctcacg
     1261 ggcggcgtcc tggacgcgca gctcggctac tggcaggggc gcctggccgg ggctccgcct
     1321 cgtctcatcc ttccctccga ccggccccgg ccggcgttgg aaacccatcg tggtgcttcc
     1381 cggccggtgg agctgactcc cgagctgtcg cacgcccttg ccacgctggc cgggcgcgag
     1441 ggcgcgacgc ctttcatggt cctggtcgca gggttcgcgg ctctcctggg ccgctattcc
     1501 ggccaggacg acgtggtggt gggctcgccc atcgcgggcc gcacccggcg ggaggtcgag
     1561 gggttgatcg gcttcttcgt caacaccctg gcgctgcggg tggagctggc ggacaggccg
     1621 gatttctcgg gtctgttgcg gcaggtgcgg cggacggcgc tggatgccta cgcccaccag
     1681 gacctcccct tcgagcgcct ggtcgaagaa ctggttacgg agcgggatct gggccactcg
     1741 ccgctgttcc aggtgatgct ggcggtgcag aacactccgg caggcccgtc ggggatgccg
     1801 ccggtgccgg ggctggagct ccgccccgtg gaggtggagg ccggaacggc gaagttcgag
     1861 ctgagcctga gcttcgctcc acgcggcgag gaactactgg gagacctcga ctacaacacc
     1921 gacctgttcg actccgccac ggcggaaagg ctggcgcgac acttcgtgcg gctgctggaa
     1981 ggggcggtgg cggccccgga gatcccgctg ccggagctct ctctcctggc tccatccgag
     2041 cagcagcacg tcctgcgaga gtggaatgac acggcgacgg attacggtgc ggagtccgga
     2101 gtctgcctgc acgggctggt gagggctcag gtggagcgcg ctccggacgc ggtggcggcg
     2161 gtgtcggagg acgagcacct gagctatcgc gaactggcgc ggcaggccgg ctgcctggcg
     2221 ccccgtctga cggaggtggg ggccggcccg gagtcggtgg tggcgatctg cgccgagcgg
     2281 tcgctggaga tgatcgtggg catgctcggg atcctcgagg ccggaggggc ctatctgccg
     2341 ctcgaccctt cgtatccgcg ggagcgtctg gcgctgatcc tggagagctc cgcggcggcg
     2401 gcggtgctgg tccaggacca tctgcaggac cgcctgccgg cggcgcccgt tccgcggctg
     2461 ccgctcggtc cgggtcgcga gggcctggcc tggatgcctc cggaggcccg gcccgagaac
     2521 ctggcctacg tcatcttcac ctcgggctcg acgggcacgc ccaagggggc gatggtgccg
     2581 caccgggcga tcgtcaaccg tctgctctgg atgcaggcgg cctatggcct gagcccggcg
     2641 gaccgggtgc tgcagaagac gccgttcggc ttcgacgtat cggtgtggga gtttttctgg
     2701 cccctcctgg tgggggcgcc cctggtgttc gcccgtcccg agggtcacaa ggaccccggt
     2761 tacctggcgg cgctgatcgc cgagcagggg atcaccgttc tgcacttcgt cccctcgatg
     2821 ctgcgcgcct tcctggagac ggaccgccag gagagctgtg tttccgtccg ggcggtgatg
     2881 gctagcggcg aggcgctgcc gctcgacctg acggagcggt tcttcgagtg gatgccggga
     2941 gagctgcaca acctctacgg acccaccgaa gcggcggtgg acgtcagctc ctgggcctgc
     3001 gcgccggcgg aggagcggcg atcggtcccg atcggccgtc ccatcgccaa cctgcgtctg
     3061 catgtcaccg accggagctg gcagccggcg ccgatgggcg tggccggaga gctgttgatc
     3121 ggcggcgtcg ggctggggcg cggctacctg gggcgaccgg agctgaccgc ggagcggttc
     3181 gtgcccgacc cgttcgccgc cctgtggagt gagccggggg cgcgggtcta ccggaccggc
     3241 gacctggccc ggcaactgcc gggcggcgag atcgatttcc tggggcggat cgacttccaa
     3301 gtcaagatcc gcggcttccg gatcgagctg ggggagatcg a
//
LOCUS       DASPJI010000220         6340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_414523, whole genome shotgun sequence.
ACCESSION   DASPJI010000220 DASPJI010000000
VERSION     DASPJI010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414523"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VEL74_05780"
     CDS             <1..276
                     /locus_tag="VEL74_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62069.1"
                     /translation="PAPGGWVVRARWSVLPAEGEGDGAFRETFPQEPPFGRAADLAIE
                     VVDQDGGQEQPTVLTTGLEGKDGASILLLDADPGAPGLFEMLKIKGC"
     gene            326..517
                     /locus_tag="VEL74_05785"
     CDS             326..517
                     /locus_tag="VEL74_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HYG62070.1"
                     /translation="MQRQLTMVYWRGEKHWLGKLLEHPDIMTQGETLEELEVNLRDAF
                     KEMVLEDVPEGHQIKQIAV"
     gene            514..696
                     /locus_tag="VEL74_05790"
     CDS             514..696
                     /locus_tag="VEL74_05790"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HYG62071.1"
                     /translation="MRRAEFVRQLVRDGCVLHRHGGRHDIYVNPATGQKQPVPRHSEI
                     DNALAKHIRKYLGLPS"
     gene            790..1008
                     /locus_tag="VEL74_05795"
     CDS             790..1008
                     /locus_tag="VEL74_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62072.1"
                     /translation="MRKKLLFLASAVLTTAALALPVPRAEAEVTCSLRCICGVVICTC
                     TDGSECTIQPPYPPIPCIWINCDGGELD"
     gene            complement(1005..1559)
                     /locus_tag="VEL74_05800"
     CDS             complement(1005..1559)
                     /locus_tag="VEL74_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HYG62073.1"
                     /translation="MAPIFTVGHSTRSIEELLALLSEPGVEVLVDVRRYPGSRRHPQF
                     SRDALEASLAAAGVGYVHEPDLGGRRPARPGSPHTAWRVEAFRGYADHMETPEFGAAL
                     ERLIRLGSERVTVILCAEAVPWRCHRRLISDALVARGIEVRHILGPGRADLHELDPNA
                     RVMPGGRLVYSGPEGDQGSLFEAP"
     gene            1611..2348
                     /locus_tag="VEL74_05805"
     CDS             1611..2348
                     /locus_tag="VEL74_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYG62074.1"
                     /translation="MQIRVLTYNIHRAIGVDRRFRPERVAGIIAHYDPDLVLLQEVDE
                     GAPRSRELDLAKEMAHVLNFPYFAVGHNVSLRKGRYGNATLSRFPIVRERNIDLTIGI
                     RKRRGCQHTSLVLGEGRHLEVFNLHLGLSARERHRQIELLVRCSEFVHVDGDTPVLVG
                     GDFNDWRSLLRPAFTNGLGFRSATDNRRFGTDWAMPTYPSFSPQGALDRIYYRGPIEL
                     LTVRRCRLQASRVASDHLPVIADFDLR"
     gene            2478..4313
                     /locus_tag="VEL74_05810"
     CDS             2478..4313
                     /locus_tag="VEL74_05810"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HYG62075.1"
                     /translation="MPRPAVLLVDPEVSRRKDLAHGLSGFGYEVIPAVDEQEGRRFAA
                     GLGPGVVVAPAELARSGGPVTERFAGIPGEGAASRHTLLLLGKGEEDGRDLPEEVLFL
                     QANGFPPSDLVRRIHLVLLGREIGLEPDAGLDSLVGDLSLVSLFELLRDLQRARASAR
                     VVCAEGEVTFEEGEVISATAGHARGLKAFFRLGSLSAGPFWVQLRDPRQSQAGRREIQ
                     EDLKALIIRALEEQVHDAPDPRTRVRIEMGPSFFDTRFNPRQQELLAALPSCPTVGRL
                     LDALPASDGEILRDLLELRELGLLRLEEPQDLVRVVTDSTSDLPPELARAHGIHVVPL
                     LVLFGDRVYHDGVDLRPRELYEQLEKGEVHPKTNPPLQRDFLEAYGGLAGRKDLISVH
                     LSGKLSQTLEHARAAVAEGLPSFQDARGPAERVTIKVVDSGNVSLGLGMMALFAARMA
                     RRGVEPDVIAERLEAMRGRMHVLFAVDSLDYLARGGRIGKGRALMGNLLGIKPILGVV
                     DGEVVSLDKVRGGRAAQPRLVELFRQRVDPSRPVMASIAHAKAPVWADRLRGLLQQSF
                     QTVELIVAEMGPVVGTHAGPGTVGACLFQPTDEELTLIAPLPETL"
     gene            4314..5009
                     /locus_tag="VEL74_05815"
     CDS             4314..5009
                     /locus_tag="VEL74_05815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG62076.1"
                     /translation="MSNDVRTIVLTGATKGLGRALSRCLAEMGHVVLGCGRSRERVEE
                     LRGELGGQHRFDVVDVASAAEVHAWAERLLARYPAPDLLLNNAGLINAGAPLWEVFEE
                     EFSRVVDVNLKGTYHTIRAFLPAMLERGRGVVVNFSSGWGRSTSPEMAPYCATKWAVE
                     GLTKALAQELPSGVAAVALNPGIIHTEMLETCFGQSAKSYPGPAKWADRAAPFLLGLG
                     PEHNGKSLTVPGF"
     gene            5068..5505
                     /locus_tag="VEL74_05820"
     CDS             5068..5505
                     /locus_tag="VEL74_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62077.1"
                     /translation="MSDDRKYRQRGYHDSDRGAQPSRGPMPPREKKEGPRGRGLGAPS
                     ESVFRCAHCNEKRPLFEELTVDAVCGKCNTSLHTCATCIHFDTSVRWECRQVESLPAR
                     VAKKRDRNECAFFTPKTVQEFAKESEKGSPGDARSAFDALFKF"
     gene            complement(5548..>6340)
                     /locus_tag="VEL74_05825"
     CDS             complement(5548..>6340)
                     /locus_tag="VEL74_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014034600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYG62078.1"
                     /translation="DMYNYTAGATGLDAAMPPWVQEGGLEAWKKRLQDPAIRERIMKE
                     MRTPTDEWENLLLSAGSPDNVLLVAFKSDALKPLTGKTLAEVAKMRGKSPEETAMDLV
                     IEDDSRVGTVYFLMSEDNVRRQIAIPWVSFGSDAEALAPEGVFLKSNPHPRAYGNVAR
                     LLGRYVRDEKIIPLEEAVRRLTSLPAENLKIERRGRLREGYYADLVVFDPAKVQDHAT
                     FDKPHQYSTGVRHVFVNGVQVLKDGEPTGATPGRVVRGPGYKKKM"
BASE COUNT          929 a         2194 c         2235 g          982 t
ORIGIN      
        1 ccggcgccgg gcggctgggt cgtgcgggcg cgctggtcgg tgctccccgc cgagggggaa
       61 ggcgacggcg cgttccggga gaccttcccc caagagccgc cgttcggccg cgccgccgat
      121 ctggccatcg aggtcgtcga ccaggacggc ggccaggaac agccaacggt cctgaccacc
      181 ggcctggagg gcaaggacgg cgcctccatc ctcctcctcg acgccgaccc cggcgcgccg
      241 gggctcttcg agatgctgaa gatcaagggg tgctgagcct ttccctgcca ggatggagta
      301 ggcttgacct ggtgaggagc agcgaatgca acggcaactg acgatggtgt actggcgcgg
      361 agagaagcac tggctgggga agctcttgga gcatcccgac atcatgaccc agggcgagac
      421 gctggaagag ctggaagtga atcttcgcga tgctttcaaa gagatggtcc tcgaggatgt
      481 ccccgagggc catcagatca agcagatcgc cgtttgaggc gagccgaatt cgtccggcag
      541 cttgtccgcg atggttgtgt gctgcatcgc catggtgggc gccatgacat ctacgtgaat
      601 ccggctacag gccagaaaca gccggttccc cgccactccg aaatcgacaa cgctctcgcc
      661 aagcacatca ggaagtacct cggcctaccg tcctgaccca gggctgccag ggcgccggtc
      721 cgggagcgcg gcgcacctct tccttcagaa gccgccaaca cctcgttgcg ggctcttgga
      781 gggtccccca tgcggaagaa gctcctgttc cttgcctccg ccgtcctcac caccgcggcg
      841 ctggccctgc cggtaccccg cgccgaagcc gaggtcacgt gcagccttcg atgcatctgc
      901 ggcgtcgtga tctgcacttg caccgatggc agcgagtgca cgatccagcc tccctacccg
      961 ccgatcccct gcatctggat caactgcgac gggggagagc tggactaggg ggcctcgaac
     1021 aggctcccct gatccccctc gggtcccgag tagacgagcc ggcctccggg catgaccctg
     1081 gcattcgggt ccagctcatg gaggtcggcg cggccggggc ccaggatgtg acggacctcg
     1141 atccccctcg ccaccagggc atccgagatc agccgccggt ggcagcgcca ggggacggcc
     1201 tcggcgcaga ggatcaccgt cacccgctcc gagccgagcc ggatcagcct ctccagggcg
     1261 gcaccgaact ccggagtctc catgtggtcg gcgtagccgc ggaaggcttc cacccgccag
     1321 gcggtgtggg gggagccggg gcgcgccggc cgccggcccc ccaggtccgg ttcatgcacg
     1381 tagcccacgc cggcggcagc cagggacgcc tccagggcgt ccctggagaa ctggggatgg
     1441 cggcgcgagc cggggtagcg gcggacgtcc accagcacct cgacgccggg ctcggagagg
     1501 agcgccaaaa gctcctcgat ggagcgcgtc gagtgaccca cggtgaagat cggcgccatg
     1561 cggctactat atgcctgccc tgagggggga gaggcaacgg agaggagacg ttgcagatcc
     1621 gagtccttac gtacaacatc catcgggcca tcggtgtgga ccgccgcttc cggcccgagc
     1681 gcgtcgcggg gatcatcgct cactacgacc ccgacctggt cctgctccag gaggtggacg
     1741 aaggggcgcc gaggtcgcgc gagctggact tggccaagga gatggctcac gtcctcaact
     1801 tcccctactt cgccgtaggc cacaacgtca gcctgcgcaa ggggaggtac ggcaacgcca
     1861 ccctcagccg gttccccatc gtgcgcgagc gcaatatcga cctcaccatc ggcatccgca
     1921 agcggcgggg gtgccagcac acgagcctgg tgctggggga ggggaggcac ctggaggtgt
     1981 tcaacctcca cctcggcctc tccgcccgcg agcgccaccg gcagatcgag ctcctggtcc
     2041 gctgctccga gttcgtccac gtcgatggcg acacgcccgt gctggtgggg ggggacttca
     2101 acgactggcg gtcgctcctg cggcccgcct tcaccaacgg cctcggcttc cggtccgcca
     2161 ccgacaaccg ccgtttcggc actgactggg ccatgcccac ctacccctcc ttctcgcccc
     2221 agggggcgct ggaccgcatc tactatcgcg gccccatcga gctgctcacg gtccgccgct
     2281 gccggctcca ggcctcccgg gtggccagcg accacctgcc ggtcatcgcc gatttcgacc
     2341 tccgctgaca ccctccgggc catcctctgg gatggggggc gttcccgcgc cggcccggag
     2401 ggtttatgct tgtcgggctc ccagcatggc tgagcggggc accggccccg cgcgcgacga
     2461 cagtgaggga atcagagatg ccccgtcccg ccgtccttct ggtcgatccc gaggtctccc
     2521 gccggaagga cctcgcccac ggcctgtccg gattcggcta tgaggtcatc cccgcggtgg
     2581 acgagcagga gggccggcgc ttcgccgcgg ggctgggccc cggcgtcgtc gtggcccccg
     2641 cggagctggc gcgctccggc ggaccggtga cggagcgctt cgccggcatt ccaggcgagg
     2701 gtgcggcgtc ccgtcacacc ctgctcctcc tcggcaaggg agaggaggac gggcgcgacc
     2761 tgccggagga ggtgctcttc ctccaggcga acggcttccc gccctcggac ctggtgcggc
     2821 gcatccatct ggtccttctc gggagggaga tcggcctgga gccggacgcc gggctcgact
     2881 cgctggtcgg cgacctgtcg ctggtctcgt tgttcgagct gctgcgagac ctccagcgcg
     2941 cccgcgcctc ggcccgcgtc gtctgcgccg agggggaggt gaccttcgag gagggggagg
     3001 tgatctctgc cacggcgggg cacgcccgcg gcctcaaggc cttcttccgg ctgggatccc
     3061 tgagcgccgg cccgttctgg gtccagctcc gcgacccgcg gcagtctcag gcaggacgcc
     3121 gggagatcca ggaggatctg aaggccctca tcatccgcgc cctggaggag caggtgcacg
     3181 acgcgccgga cccgcggacc cgggtgcgga tcgagatggg gccctccttc ttcgacaccc
     3241 gcttcaaccc gcgccagcag gagctgctgg cggcgctccc ctcgtgcccg acggtgggcc
     3301 ggctgctgga cgccctcccg gccagcgacg gcgagatcct gcgcgacctc ctggagctgc
     3361 gcgagctggg tctgttgagg ctggaggagc cgcaggatct ggtccgggtg gtcaccgact
     3421 cgacctcgga cctgccgccg gagctggcgc gggcgcacgg catccatgtc gtgcctctcc
     3481 tcgtcctctt cggcgaccgc gtctaccacg acggagtgga cctgcggccc cgggagctct
     3541 acgagcagct cgagaagggg gaggtccatc cgaagaccaa tccgccgctc cagcgggact
     3601 tcctggaagc ctacggcggg ctcgcgggac gcaaggacct gatctccgtc cacctctccg
     3661 gcaagctgtc gcagacgctg gagcacgcgc gggcggcggt ggcggagggg ctcccctcgt
     3721 tccaggacgc ccgcgggccg gccgagcggg tgaccatcaa ggtggtggac agcggcaacg
     3781 tcagcctggg cctggggatg atggcgctct tcgcggcccg catggcgcgg cgcggcgtcg
     3841 agccggacgt gatcgccgag cggctggagg cgatgcgtgg gaggatgcac gtgctgttcg
     3901 ccgtcgacag cctcgactac ctggcgcgcg gcgggcgcat cggcaagggc cgggcgctga
     3961 tggggaacct gctcgggatc aagccgatcc tgggggtggt ggacggcgag gtggtgtcgc
     4021 tcgacaaggt gcggggcggg cgggccgcgc agccgcggct ggtggagctg ttccggcagc
     4081 gggtggaccc gtcgcggccg gtgatggcga gcatcgccca cgccaaggcg ccggtctggg
     4141 ccgaccgcct gcgggggctg ttgcagcaga gctttcagac ggtcgagctg atcgtcgccg
     4201 agatgggccc ggtggtgggc acccatgccg ggccgggtac ggtgggcgcc tgtctcttcc
     4261 agcccacgga cgaggagctg accctgatcg cccccttgcc ggagaccctg tagatgagca
     4321 acgacgtccg taccatcgtc ctcaccggcg cgaccaaggg tctgggccgg gcgctgtccc
     4381 gctgcctggc ggagatgggt catgtggtcc tgggctgcgg ccggtcgcgc gagcgggtgg
     4441 aggagctgcg gggggagctc gggggccagc accgcttcga cgtggtggac gtggcctcgg
     4501 ccgccgaggt ccacgcctgg gccgagcgcc tcctcgcccg ctatccggcg cccgacctgc
     4561 tgctcaacaa cgccggcctg atcaacgccg gcgcgccgct ctgggaggtc ttcgaggagg
     4621 agttctcgcg ggtggtggac gtcaacctca aggggaccta ccacaccatc cgcgccttcc
     4681 tgccggcgat gctggagcgg ggccgggggg tggtggtcaa cttcagctcc ggctggggcc
     4741 gctccacctc gccggagatg gccccctact gcgccaccaa gtgggcggtg gagggcctga
     4801 ccaaggcgct ggcgcaggag ctgccgtccg gagtcgcggc ggtggcactc aacccgggga
     4861 tcatccacac cgagatgctg gagacctgct tcggccagtc cgccaagagc tatcccggcc
     4921 ccgccaaatg ggccgaccgc gccgccccct tcctcctcgg cctcggcccc gagcacaacg
     4981 gcaagagcct gacggtgccg gggttctgag gaatagtccg gccgcgcgag ctgtaatatt
     5041 catagcttca cagaaaggcg caccaccatg tccgacgacc gcaagtaccg ccagcgtggc
     5101 tatcacgata gtgatcgggg ggcccagccg tctcgcgggc cgatgccgcc gcgggagaag
     5161 aaggaggggc cgcgggggag agggttgggg gcgccgtccg agagcgtgtt ccggtgcgcc
     5221 cattgcaacg agaagcggcc gctgttcgag gagctgacgg tggacgcggt ctgtggcaag
     5281 tgcaacacct ctctccacac ctgcgctact tgcatccact tcgacacatc ggtccgctgg
     5341 gagtgccggc aggtggagtc cctgccggca cgggtggcca agaagcggga ccgcaacgag
     5401 tgcgccttct tcacccccaa gacggtccag gagttcgcca aggagagcga aaagggctct
     5461 ccgggcgatg ccaggtcggc cttcgacgcg ctgttcaagt tctaggcggg tgggtcgctc
     5521 cttcccccgc cccctccaga cccaccccta cattttcttc ttgtatcccg gcccccgcac
     5581 cacccttccg ggggtggcgc cggtgggctc gccgtccttg agcacctgga cgccgttgac
     5641 gaagacgtgc cggacgccgg tcgagtactg gtgcggcttg tcgaaggtgg cgtggtcctg
     5701 caccttggcc ggatcgaaga ccaccaggtc ggcgtagtag ccctcccgga gccggccgcg
     5761 ccgctcgatt ttcaggttct ccgccggcag cgacgtcaga cgccggaccg cctcttccag
     5821 ggggatgatc ttctcgtccc gcacgtagcg gccgagcagg cgggcgacgt tgccgtaggc
     5881 ccgggggtgg gggttggact tgaggaagac cccctccggg gccagcgcct cggcgtccga
     5941 gccgaagctc acccagggga tggcgatctg gcgccggacg ttgtcctccg acatcaggaa
     6001 gtagacggtg cccacccggc tgtcgtcctc gatcaccagg tccatggccg tctcctcggg
     6061 ggacttgccg cgcatcttcg ccacctcggc cagggtcttg ccggtcagcg gcttcagggc
     6121 gtcgctcttg aacgccacca gcaggacgtt gtcggggctg ccggcggaga ggagcaggtt
     6181 ctcccactca tcggtcggcg tcctcatctc cttcatgatc cgctcgcgga tggccgggtc
     6241 ctgcagccgc ttcttccagg cctcgagccc cccctcctgc acccaggggg gcatggcggc
     6301 gtcgagtccg gtggcgccgg cggtgtagtt gtacatgtcg
//
LOCUS       DASPJI010000221         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_129300, whole genome shotgun sequence.
ACCESSION   DASPJI010000221 DASPJI010000000
VERSION     DASPJI010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129300"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1494)
                     /locus_tag="VEL74_05830"
     CDS             complement(<1..1494)
                     /locus_tag="VEL74_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62079.1"
                     /translation="MRFQRLLALLLLTAVALPGLPARGATPSAPFLLDEEPRCTETAP
                     DVAFLPGGGFAAVWGGSRPQPLLASPAVYLRLFDRDGRPLGPETLLTSETEQPARPAV
                     AANAGGTFLVVWGDSRGEGTVQGRLFNASGSSLGEAFEISARSPRGGVDVTAEPGGGF
                     AVVWANKDSILLRRYGPTGEALDAEQWLLGFAGVPEDTFPQGYDPAVVAHGPAGLAVA
                     FSDAAARSFSDPTLRGGNLGLMLVKPGQEPPYIHIGTFGLDPVPVPQRLPRPSLAVGA
                     AGRVFVVWREQTPEGLVLRGRIYTALGDPLGDAFRLDLGAWDELSGPSVATDGSGNFL
                     LAWLGAPTGAQPEGARVYVRRFGADGNPLGGQEPVSSLFPSFDTTTSPALTVDPMGRA
                     LIAWGTGRPLPPIVGAPFQCFSEGIYGQILDPATPLDLPLQGGRFLAGVRWADHAGRT
                     GVGQPVALTGDSGYFWFFDENNAELVVKVLDGRAVNGHYWVFYGALSD"
     gene            complement(1510..>2185)
                     /locus_tag="VEL74_05835"
     CDS             complement(1510..>2185)
                     /locus_tag="VEL74_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62080.1"
                     /translation="ALTGDSGYFWFFDENNAELVVKVLDGRAVNGHYWVFYGALSDVA
                     YTLEVRDTLTGEVKTYTNPAGRLTSRADTMAFAVPGQAGAPVLSAPNELGFLASSGLA
                     TGKAFPTVGFCGDNVTTPSTGFCLGGRFDVAVEWRANGTTGLARGAVLGYHSGYFWFF
                     GQNNVELVVKVLDGRAVNGRFWFFSGALTDVEHWIHVRDNETGEERVYHNPPGRLASR
                     ADTDAF"
BASE COUNT          361 a          765 c          785 g          274 t
ORIGIN      
        1 gtccgagagg gcgccgtaga agacccagta gtggccgttg accgcgcggc cgtccagtac
       61 cttgaccacc agctcggcgt tgttttcgtc gaagaaccag aaatagccgg agtcgccggt
      121 gagggccacg ggctgcccca ccccggtacg gccggcgtgg tcggcccagc gcaccccggc
      181 caggaagcgg cccccctgga gcggcaggtc gagaggggtt gcggggtcga ggatctgccc
      241 gtagatcccc tcgctgaagc attggaaagg cgcgccgacg atcgggggca acggccggcc
      301 ggtcccccaa gcgatgaggg cgcggcccat cgggtccacc gtcagtgccg gcgaggtcgt
      361 cgtatcgaac gacgggaaga gggacgagac cggttcctgc cctcccagag ggttgccgtc
      421 cgcgccgaac cggcggacgt agacgcgggc gccctcgggc tgcgcgccgg tgggtgctcc
      481 cagccaggcc agcaggaagt tgccgctgcc atcggtcgcg accgacggcc cggagagctc
      541 gtcccatgcg ccgagatcca agcgaaaagc gtctcccagg ggatcgccga gagccgtgta
      601 gatccggccg cgcagcacga ggccctcggg cgtctgctcc cgccagacca cgaagacgcg
      661 gccggcggcg cccacggcca gggagggcct ggggagccgc tgggggaccg gcacgggatc
      721 gagaccgaag gtgccgatat ggatataggg gggctcctgc ccgggtttga cgagcatcag
      781 gcccaggttg cctccccgca aggtcggatc ggaaaagctc cgcgcggccg cgtcactgaa
      841 ggcgacggcc agaccggcag ggccatgcgc cacgacggcg ggatcgtagc cctggggaaa
      901 tgtgtcctcg ggtacgccgg cgaacccgag gagccactgc tcggcgtcca gggcctctcc
      961 cgtggggcca tagcgccgca ggaggatcga atccttattg gcccagacca ccgcgaagcc
     1021 gccgcccggc tccgcggtca cgtccacacc ccctcgggga gaccgggccg agatctcgaa
     1081 agcctcaccg agggacgatc ccgaggcgtt gaacagccga ccctggaccg tgccctcccc
     1141 ccgggagtcg ccccagacca ccaggaacgt gccgccggcg ttcgccgcca cggcgggccg
     1201 ggccggctgc tccgtctcgc tggtgagcag ggtctcgggc cccagcggcc ggccgtcgcg
     1261 gtcgaagagc cgcagataga ccgccgggct cgccagcagc ggctggggac gggagccgcc
     1321 ccagaccgcc gcgaagccgc cgccaggcag gaaggccacg tccggcgccg tctcggtgca
     1381 gcgcggctct tcgtcgagga ggaacggcgc ggatggcgtg gcccctcgcg ccggcaggcc
     1441 cggaagggcc accgccgtca ggagcaggag cgcgagaagc ctctggaacc gcatggagat
     1501 cctcctgtgt cagaacgcgt cggtatcggc gcggctggcg agccggccgg gcggattatg
     1561 gtagacccgc tcctccccgg tctcgttgtc gcgcacgtgg atccagtgct ccacgtcggt
     1621 gagcgcgccg gagaagaacc agaagcggcc gttgaccgcc cggccgtcca gcaccttcac
     1681 caccagctcg acgttgttct ggccgaagaa ccagaaatag ccgctgtgat agccgagcac
     1741 cgcccccctc gccagcccgg tcgtaccgtt ggcccgccac tcgaccgcca catcgaagcg
     1801 gccgccgagg cagaagccgg tggaaggggt ggtcacgttg tcgccgcaga acccgacggt
     1861 ggggaatgcc ttgcccgtcg ccagcccgga ggacgcgagg aagcccagct cgttgggagc
     1921 cgagagcacc ggcgctccgg cctggcccgg caccgcgaag gccatggtgt ccgcccggct
     1981 ggtgagccgc ccggccggat tggtgtaggt cttcacctcc ccggtcaagg tgtcccgcac
     2041 ctccagggta taggccacgt ccgagagggc gccgtagaag acccagtagt ggccgttgac
     2101 cgcgcggccg tccagtacct tgaccaccag ctcggcgttg ttttcgtcga agaaccagaa
     2161 atagccggag tcgccggtga gggcc
//
LOCUS       DASPJI010000222         3792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_758506, whole genome shotgun sequence.
ACCESSION   DASPJI010000222 DASPJI010000000
VERSION     DASPJI010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758506"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(464..1444)
                     /locus_tag="VEL74_05840"
     CDS             complement(464..1444)
                     /locus_tag="VEL74_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62081.1"
                     /translation="MAKARGKTDLEAELRGLHHGPLGEFTAARNALAKQLDKSGDKDV
                     AAEVKSLPKPSVSAWAVNRLFEREPAKMQELLEAGEHARAGLRHAISGKGVESLRDAL
                     DHERTLRDALRRRAAALIEEDTGRKASPAILDRVATNLDSLALSPDSVEAAGRGWISA
                     DLAPPGFEVLSGMPLTGARTRPSHLRLVPPTRDRKEEKKGEPRKATPAKAPEKDAGPT
                     LREQRDEARAEAARRREEETERRKREREEELHRERLARLEEKAAEIRADADAAKREAD
                     RADKAVEEAERALEEARRRLENTRDDARRSHQRADRAAERLARAEKDLER"
     gene            complement(1603..2169)
                     /locus_tag="VEL74_05845"
     CDS             complement(1603..2169)
                     /locus_tag="VEL74_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62082.1"
                     /translation="MARNRSLIIASMLTLSLTSLTVSQANAQGRYGYPDSYRGSTSVD
                     RLADQIDDIAEGIYREYARNNRRPDRDERRALEALRELNVRADRFSRVMDDRRDMRHS
                     DREYDLLVRSFFETQDSLDRISRRPYVDRGMFQIGQLLRQMDRRFDRYDDRDRGRGGR
                     YDRDGRWDRYNDWDRYDRWDRGRRNGRH"
     gene            complement(2342..2968)
                     /locus_tag="VEL74_05850"
     CDS             complement(2342..2968)
                     /locus_tag="VEL74_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62083.1"
                     /translation="MRSPARPLCLSALLLVASVIPAAAEKETLQQRGLVADANFEFID
                     AAGCVVTEIAVFAVRDANRSTSAPRWRDSWANVVLFRYDLCQEEFTVLDYASGLTYLE
                     SGEAGTFAMSPGLHKATLRTRVEVSGMALHTVDLDLEWSAAGPLYNGRQRAHYEFPSG
                     MTIDTRLHGRQRLAQAAGTVWVSGERLDARSSYGNMVSTQNRTVTITH"
     gene            3155..3667
                     /locus_tag="VEL74_05855"
     CDS             3155..3667
                     /locus_tag="VEL74_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYG62084.1"
                     /translation="MKISQVMLPEFDHEMATTRRVLERIPEDKLDWQPHAKSMSLGRL
                     ASHVAELPGWVMSILKQDSLDFAPPGSPDSPRKAQVFASREEILEQFDRSIASAREAL
                     AATDDETFGKHWTLARGGKTIFNLPRAAVVRTLLLNHVIHHRGQLTVYLRLNDAPVPS
                     VYGPSADEQV"
BASE COUNT          549 a         1308 c         1318 g          617 t
ORIGIN      
        1 gcacccaagg caagccggtc cacgggcgcg gcgccgctct cgccgcgccc tggacctagt
       61 ccatcgattg tcaatgaaca tcggccgccg gtccgaccct cgccggagcc gcttcgaccc
      121 tcttcaaact actctcgacc cttctcaaac ctcgttcgac cctctccaaa cctcgcctga
      181 cccgctccaa acttcgttcg acccgcccca aagctcgctc gaccctcttc agagctgtct
      241 cgacccgggg gtcggcagag atttcagtcg ggtcgtatcc gctagcgctg cttgaaaaga
      301 accaccgaca gcttctctag tagatcgaat ccgatcccta gtgtcaatcc agcccttgcg
      361 gtagccaggc aggtccagta tagtccgcgg gcttcggtgc cggtgagggc cggagggtgt
      421 tctttgacaa ctgaatcgta gcggcgcccg ggctggcgga tcgtcagcgt tcgagatcct
      481 tctcggcccg ggccagacgc tcggcggcgc ggtcggcgcg ctggtgggat ctccgcgcgt
      541 cgtcgcgggt gttctcgaga cggcggcgcg cctcttcgag ggccctttcg gcctcttcga
      601 cggccttgtc cgcccggtcc gcctcccgtt tcgcggcgtc ggcgtcggcg cggatctccg
      661 ccgccttctc ttcgaggcgc gcgaggcgct cgcggtgcag ctcctcctcc cgctcacgct
      721 tccggcgctc ggtctcctcc tcacggcggc gcgcggcttc ggcccgggcc tcgtcccgct
      781 gctcgcggag cgtcggcccg gcgtccttct ccggcgcctt agcgggggtg gccttgcgcg
      841 gctccccctt cttctcctct ttcctgtccc gtgtcggcgg gaccaaacgg aggtggcttg
      901 ggcgggtgcg ggctccggtc aggggcatgc cggacagcac ctcgaaaccg ggcggggcga
      961 ggtcggcgct gatccagccg cggccggcgg cttcgacgga gtcggggctc agggccaggg
     1021 agtcgaggtt ggtcgccacc cggtcgagga tcgccgggct cgccttgcgg ccggtgtcct
     1081 cttcgatcag ggcggcggcg cggcgccgca gggcgtcgcg cagggtgcgc tcgtggtcca
     1141 gggcgtcgcg cagggactcg acacctttgc cggagatcgc gtgccgcagg ccggcgcggg
     1201 cgtgctcccc cgcctccagc agctcctgca tcttcgccgg ctcgcgctcg aagagccggt
     1261 tgaccgccca ggccgagacc gagggctttg gcagggactt cacctcggcg gcgacgtcct
     1321 tgtcccccga cttgtccaac tgcttggcga gggcgttgcg ggcggccgtg aactcgccca
     1381 gcggcccgtg atggagcccc ctcagctcgg cttccaggtc cgtctttccc cgtgccttgg
     1441 ccatgtcgcc tccgtgtcca cccgggggcg cagcaacgtt tgtgcctgag agggaagcct
     1501 gaagagaaaa cgccccctcc cccgcccggg gaaccccctc gaccgggagg gggaggagag
     1561 gaaaggcggg agagggggct aaaggtcgtg ggcggctacc ggtcagtgcc ggccgttgcg
     1621 gcggccacgg tcccaacggt cgtagcggtc ccaatcgttg tagcggtccc agcggccgtc
     1681 gcggtcatag cggccgccgc ggccccggtc ccggtcgtcg tagcggtcga agcggcggtc
     1741 catctgccgg aggagctggc cgatctggaa catgccccgg tccacgtacg gccggcgcga
     1801 gatccggtcc agcgagtcct gggtctcgaa gaaggacctc accagcaggt cgtactcccg
     1861 gtcgctgtgg cgcatgtccc gccggtcgtc catgacgcgg gagaagcggt ctgcccggac
     1921 gttcagctcc cgcagcgcct ccagagcccg ccgctcgtca cggtccggac ggcggttgtt
     1981 gcgggcgtac tcgcggtaga tgccctcggc gatgtcgtcg atctggtcgg ccagccggtc
     2041 gaccgacgtg gagccccgat acgagtcggg atagccgtac cggccctggg cgttcgcctg
     2101 ggagacggtc agcgaggtca ggctcagggt gagcatggaa gcgatgatca gactgcgatt
     2161 acgtgccatg gtttggtttc tcctaagttg gccaaccctc tatagggcaa gccggatgcc
     2221 aaggagaggc acgttgaaaa caaaggactt acaggaaagg gagggagaga gggggcccga
     2281 tctcgtcctc tggagaggac gcggtgacac ccccggcggg ctgcccgccg gggggacccg
     2341 ttcagtgggt gatcgtgacg gtccggttct gcgtggagac catgttgccg tagctggagc
     2401 gggcgtcgag acgctcgccg gagacccaga ccgtgcccgc cgcctgggcc agacgctggc
     2461 ggccgtgcag ccgggtgtcg atcgtcatcc cgctggggaa ctcgtagtgg gcgcgctgcc
     2521 gcccgttgta gagcggcccc gccgccgacc actcgaggtc gaggtccacc gtatggaggg
     2581 ccatgccgct gacctccacc cgggtccgca gagtggcctt gtggagaccg gggctcatcg
     2641 cgaaggtgcc cgcctcgccg ctctccaggt aggtgagccc ggaggcgtag tccaggaccg
     2701 tgaactcctc ctggcagagg tcgtaccgga acagcaccac gttcgcccag gagtcccgcc
     2761 accggggagc cgacgtcgac cggttcgcgt cgcggacggc gaagacggcg atctcggtga
     2821 ccacgcagcc ggcggcgtcg atgaactcga agttggcgtc ggcgacgagc ccccgctgtt
     2881 gcagggtctc cttctcggcg gcggccggga tgacgctggc aacgagcaga agggcggaca
     2941 gacagagcgg gcgggcgggg cttctcatgg tgcggcgact ccttactgcg gtcagcgctg
     3001 gactaccctc tccttcgtgg agggatgtcc atttgaatag caattgaagt gccagacaga
     3061 ggccgttgcc gccctcgcgc gttttctcta ggatgcgagg cgcccctcac caggggcaga
     3121 cgcagacgca gaccggagga acgaaatggg tgagatgaaa atcagtcaag tgatgctgcc
     3181 cgagttcgac cacgagatgg ccaccacccg ccgggtcctg gagcggatcc cggaggacaa
     3241 gctcgactgg caaccgcacg cgaagtccat gagcctcggc cggctcgcca gccacgtggc
     3301 ggagctgccc ggctgggtga tgtccatcct gaagcaggat tcgctggact tcgcgccgcc
     3361 cggcagcccg gactcgcccc gcaaggcaca ggtcttcgcc agccgcgagg agatcctgga
     3421 gcagttcgac cgcagcatcg ccagcgcccg cgaggcgctc gccgccaccg acgacgagac
     3481 gttcggcaag cactggaccc tggcccgcgg gggcaagacg atcttcaacc tgccccgcgc
     3541 cgccgtggtg cggacgctgc tcctcaacca cgtcatccac caccgcgggc agctcaccgt
     3601 ctacctgcgc ctgaacgacg ccccggtgcc ctcggtctac gggccgtcgg ccgacgagca
     3661 ggtgtagagg ccgatggggc gcctgctggg catcctcggg gggatggggc cgctgccgtc
     3721 ggcggagctc gtccacacgc tctaccgtct caacccggcg gcgaccgagc aggggatgcc
     3781 cgcctgcgtc cc
//
LOCUS       DASPJI010000223         7818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_758643, whole genome shotgun sequence.
ACCESSION   DASPJI010000223 DASPJI010000000
VERSION     DASPJI010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7818)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758643"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2323)
                     /locus_tag="VEL74_05860"
     CDS             complement(<1..2323)
                     /locus_tag="VEL74_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG62085.1"
                     /translation="MKGPAVPPAAPDLENLTPEQRALLMLRLRRQAQRGPEGAGAAPA
                     PRVTSIPRAPRDRPLPLSFGQRRLWLTDRLQPGNPAYNVPFAFRVAGRLDGVGLDAAF
                     AGIVRRHESLRTRFVADPLEDVEPLQVVGPPAPVPVPRVDLGNLPEDTRLAEARRLAA
                     EVARHPFELESGRLLRVVLLALGTDDHALLTVLHHIAADAWSIGIFIREFIALYQAHL
                     EKRPPALPELPVQYPDYAAWQREHRAEELAAQASFWKERLSGIPALDLPADRPRPAAL
                     SGRGAQTSRWWQQPVLEGLRAFARRQGVTVFMSLLAAFQALLARSTGQDDVSVGTPVA
                     GRGREELEPLIGFFINTLVLRTDLSGDPSFSALLARVREATVEAFAHQDVPFEQVVEA
                     VRPGGAPLFRVMFNYQSRPEVSAGIPGVELSRLQFEAGVSMFDLTLDCREIGDSLLVE
                     TEYSTDLFDAPTVERLLGHLETLLAAALAGPMRPFSELPLLSPAERHAVLTEWNDTPG
                     ETWDTKGFVHAQFADQARRAPDSLALVFGEEGEGAWTYAELRRRAGSLARRLRSLGVG
                     PEVRVGICAGRTPERVASVLAVLKAGGAFVPLDPDDPRERLAWIFEDARPAVLLAEER
                     LRGRLPEGARFLPLESAWEEGGDPPDCPRLSPENAAYVIYTSGSTGRPKGVVVPHRGI
                     AASARAWQEEYGLTGRVRSLLQVAGFSFDVFIGDLVRALTTGAELVLAPAGVLTDPAL
                     LHGWLSRRRIDFADLVPAVARALVEHLEAVDATL"
     gene            complement(2323..3918)
                     /gene="serA"
                     /locus_tag="VEL74_05865"
     CDS             complement(2323..3918)
                     /gene="serA"
                     /locus_tag="VEL74_05865"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HYG62086.1"
                     /translation="MHRILIADSLDPSGLEMLRASGAEVRLLQKEERPRLPEIVEGFD
                     ALVVRSATKVTADLLRAGKRLKVVGRAGIGVDNVDVAAATDLGILVVNAPTANLMSAT
                     EHTWALLLALARKVPQADVSTKSGGWDRKILGTELQGKTLGVVGFGRIGQRVAARARG
                     FEMKVVAYDPFLDPEVARRLEVALLPVEDLLRQADVVTIHTPLTRETRNLLSRERLAL
                     MKPGALLVNCGRGGLVDEEALLAALESGAIAGAALDVFAEEPPTDLRLVQHPRVVATP
                     HIGAQTREAQDRISTETAEMVLAALAGSLTVAAVNLPFRAAGSGAEPYLALGELLGRM
                     AGLLLEAPPKRLQVDLWGIDEALRVPCSVAVVKGMLTPFLGEAVNYVNAERLAQSRGI
                     EVVRSTHSAPGDYPQLVGVALAGGGRSVELDGTLFNERDPRVVRFGGFRLEFRPQGKL
                     LVLENRDVPGVVGRVGTLIADAGINIADIHLAQRGRGQEALAVLRLDQDPDAELMARL
                     SALPEVRSARRVDLGEPGPRPAE"
     gene            complement(3921..5006)
                     /locus_tag="VEL74_05870"
     CDS             complement(3921..5006)
                     /locus_tag="VEL74_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HYG62087.1"
                     /translation="MNEQIQFFLPGPTYVPEDARQAMTKPMAGHRSAGFKKFYLHLSE
                     RLPALLRTQGDVMVATGSSTLIMESAVVSCAASDVLNLTCGAFSERWHSIAKSLGKNA
                     DKVAVPWGQPMDPEVVRAALRRKKYDAVTMVHNETSTGVMSPIADVARVIREESDALI
                     LVDTVSSLGGAPVETDAWGLDVVLAGTQKAIAVPPGLTVFTLSDRAAERAAKVPHRGF
                     YTDLLRYRDKHREGGFITTPGIPLLYALDVQLDRILAEGMEARWERHRRLEQRTEEWA
                     AARGCSYASGAEGRSWTVSCLHPPAGVDPQAIVKRLADRGWTIGGGYGDWKPTTFRIG
                     HMGEVRENHLEALLAQIDEVLGELAAA"
     gene            5211..5513
                     /locus_tag="VEL74_05875"
     CDS             5211..5513
                     /locus_tag="VEL74_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HYG62088.1"
                     /translation="MRVISLSTLKAFWEGNPEYADSREPVLAWYRHVLRADWDSPAAV
                     KEEFRHASILRDGRVVFNIAGNKYRLVVWINYRYRVVYIRFLGTHSQYDQIDAQTI"
     gene            5531..5905
                     /locus_tag="VEL74_05880"
     CDS             5531..5905
                     /locus_tag="VEL74_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010319432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HYG62089.1"
                     /translation="MDIKPIRTEQDYRATLQEIESLMNAEPGSPEGERLDILVTLLEA
                     YEQKHFPMDLPDPVEAIKFVMDQRGLTVKDLEPMIGRPNRVYEVLSHKRPLTLKMIWR
                     LHKDLGIPAECLIKQPEEHRAA"
     gene            complement(5927..6337)
                     /locus_tag="VEL74_05885"
     CDS             complement(5927..6337)
                     /locus_tag="VEL74_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62090.1"
                     /translation="MKSPHLLRVEEGPERFAPLIEAARAEGLRVGWLDLAPPPPMAGD
                     LETAAAAGVLRAVAVGGDRSVVVKPLRGAPVLQDLLREHFRGTALVLVRGELEAPLLR
                     WVGEEWEVTVPGQAAVRLETGRLVPALRKPRPWA"
     gene            complement(6334..7521)
                     /locus_tag="VEL74_05890"
     CDS             complement(6334..7521)
                     /locus_tag="VEL74_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010046421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HYG62091.1"
                     /translation="MRTGLDRLLADPSRLAGRRYGLLAHGASITREPRPIHRALAALS
                     ADGEAPRALFGPEHGYYGVEQDMVASENERDPWTGVPIVSLYGDREESLKPCPCAFDG
                     LDLLVIDLQDVGSRYYTYAATAVWAAEAALAAGCEVWVLDRPNPIGGVIEGDRPRPGH
                     ESFVSAFLLPVRHGLTLGEILRLEARRRGWKEGLEVWAMEGWRRDMPWEATGLPWVAP
                     SPNMPTLLTALIYPGGCLVEATELSEGRGTTRPFQLVGAPGLDPVALADRLAARGLPG
                     IAFLPTYFKPQFQKHRGQVCGGVELVVTDAAALRPYRTGVELLFTVRQLDPDRFAWRE
                     APYEFVSDRPAIDLLTGGPECREAIDTGDEKALTGWIAGWAADEAAFREEYQDILLYE
                     DGA"
     gene            complement(7521..>7818)
                     /locus_tag="VEL74_05895"
     CDS             complement(7521..>7818)
                     /locus_tag="VEL74_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010551188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HYG62092.1"
                     /translation="GIQGAKRTAEMIPLAHPLPLDQVEVELQPIPEGVEIRSRVVVTG
                     RTGAEMEAMLACSTAALALYDMVKAQERGAVITDLRLEAKSGGKSGSYRRQGGA"
BASE COUNT         1198 a         2768 c         2830 g         1022 t
ORIGIN      
        1 cgagggtggc gtccaccgcc tccaggtgct ccacgagggc gcgcgccacc gccggcacca
       61 gatcggcgaa gtcgatccgc cggcgcgaga gccagccgtg cagcagcgcc gggtcggtca
      121 gtacccccgc cggcgcgagc accagctccg ccccagtggt cagggcgcgc accaggtcgc
      181 cgatgaagac atcgaacgag aaccccgcca cctggagcag ggagcggacg cgtccggtca
      241 acccatactc ctcctgccag gcgcgggcag aggcggcgat gccgcggtgg ggcaccacca
      301 cccctttggg cctccccgtc gagccggagg tgtagatcac gtaggccgcg ttttccggag
      361 agagccgagg acagtccggg ggatcgcccc cctcttccca ggcggactcc agaggaagga
      421 agcgcgcgcc ctccggcagc cggccgcgca gccgctcctc ggcgagcaga accgccgggc
      481 gggcatcttc gaagatccag gccagacgct cgcgtgggtc gtccgggtcg agcgggacga
      541 acgctccgcc ggctttcagc acggcgagta cggaggccac ccgctccgga gtccggccgg
      601 cgcagatccc cacccgcacc tcgggaccga ccccgagaga gcgcaggcga cgtgccaggc
      661 tccccgcccg gcggcgcagc tcggcgtacg tccacgcgcc ctccccttcc tcgccgaata
      721 ccagggccag agagtccggg gcgcggcggg cctggtcggc gaactgagca tggacgaagc
      781 ccttcgtatc ccaagtctct ccgggggtgt cgttccactc ggtaagcaca gcgtgtcgct
      841 cggctgggga gaggagaggc agctccgaga acggccgcat ggggccggcc agggccgcgg
      901 cgagcagcgt ctcgaggtgg ccgagcaggc gctcgaccgt cggcgcgtcg aacaggtcgg
      961 tgctgtactc ggtctcgacc aggagggagt cgccgatctc ccggcagtcc agggtgagat
     1021 cgaacatcga gaccccggcc tcgaactgga gccgcgacag ctccacgccg gggatccccg
     1081 ccgacacctc gggccggctc tgatagttga acatcacccg gaacagcggc gccccgcccg
     1141 gccgcaccgc ctccaccacc tgctcgaacg gcacgtcctg gtgggcgaag gcctccaccg
     1201 tggcctcccg cacccgcgcc agcagggcgc tgaacgacgg gtcccccgac aggtccgtgc
     1261 gcaggaccag cgtgttgatg aagaaaccga tgagcggctc cagctcctcc cggccacggc
     1321 cggccaccgg tgtgccgacg ctcacgtcgt cctggccggt ggagcgggcc agcagcgcct
     1381 ggaaggccgc cagcagactc atgaagacgg tgacgccctg acgccgggcg aacgcccgga
     1441 gcccctcgag aaccggctgc tgccaccaac gggaagtctg ggctccccgt ccactgagcg
     1501 ccgccggccg cgggcggtcc gccgggaggt ccagcgccgg gatgccgctc aggcgctcct
     1561 tccagaagga agcctgcgcc gccagctcct cggcccggtg ctcgcgctgc caggcagcgt
     1621 agtccgggta ctggaccggc agctccggca gggcaggtgg gcgcttctcg aggtgggcct
     1681 gatagagggc gatgaactcg cggatgaaaa tcccgatcga ccaggcgtcg gccgcgatgt
     1741 ggtggaggac cgtgagcagg gcgtgatcat ccgtccccag cgcgagcagg acgacccgca
     1801 gcaggcgccc gctctccagc tcgaaggggt ggcgcgccac ctcggcggcc agccggcggg
     1861 cctcggccaa gcgcgtgtcc tccgggagat tcccgaggtc gacgcgtggc accggcaccg
     1921 gagcgggcgg cccgactacc tggagcggct cgacgtcctc cagaggatcg gcgacgaacc
     1981 gggtgcgcag gctctcgtgg cggcggacga tgccggcgaa ggccgcgtcc agacccacgc
     2041 cgtccagccg gccggcgacc cgaaaggcga acggcacgtt gtaggccgga ttccccggct
     2101 gcagccggtc ggtcagccag agccgccgct gcccgaagga gagggggagc ggccggtcgc
     2161 gcggggctcg ggggatggag gtgacgcggg gggccggcgc ggcgcccgcc ccctccgggc
     2221 cacgctgggc ctggcgccgc agccgcagca tgagcagcgc ccgctgctcg ggggtcaggt
     2281 tttcgagatc aggggcggcc gggggtacag caggcccctt catcactcgg ccgggcgggg
     2341 accgggctca ccgaggtcga cgcggcgggc gctccgcacc tcggggaggg cggacaggcg
     2401 cgccatcagc tcggcgtcgg ggtcctgatc gaggcgcagg acggcgaggg cctcctgacc
     2461 gcgccccctc tgcgcgaggt ggatgtcggc gatattgatt cccgcgtcgg cgatcagggt
     2521 ccccacccta ccgaccacgc cggggacatc gcggttctcg agcaccagca gcttgccctg
     2581 cggccggaac tccagccgga agccgccgaa acggaccacc cgcggatcgc gttcattgaa
     2641 gagagtcccg tcgagctcga cgctccgccc gccgccggcc agggccacgc cgacgagttg
     2701 ggggtagtcc cccggcgcgc tgtgggtgga gcgcaccacc tcgatgccgc ggctctgcgc
     2761 caggcgctcg gcgttgacgt agttgaccgc ctcgcccagg aagggcgtca gcatcccctt
     2821 gaccacggcc accgagcagg ggacgcgcag cgcctcgtcg ataccccaga gatccacctg
     2881 caggcgcttc ggcggcgctt cgaggagcag cccggccatg cgcccgagga gctcgcccag
     2941 cgccaggtaa ggctcggcgc cgctgccggc ggcgcggaac ggcaggttca ccgcggccac
     3001 cgtcaaggag ccggccaggg cggcgaggac catctccgcg gtctcggtgg agatgcggtc
     3061 ctgggcctcg cgggtctggg cgccgatgtg cggcgtcgcc accacgcgcg gatgctgcac
     3121 cagacgcagg tcggtgggcg gctcctcggc gaagacgtcg agcgcggcgc ccgcgatggc
     3181 cccggactcc agagccgcga ggagcgcctc ctcgtccacc aggccgccgc ggccgcagtt
     3241 gaccagcagc gcgccgggct tcatcagggc aaggcgctcg cgcgagagga ggttgcgggt
     3301 ctccctggtg agcggcgtgt ggatggtgac cacgtcggcc tgccgcagca ggtcctccac
     3361 ggggagcagc gccacctcca ggcggcgcgc cacctccgga tcgaggaacg ggtcgtaggc
     3421 gaccaccttc atctcgaagc cgcgggcgcg ggcggcgacg cgctggccga tccgcccgaa
     3481 gccgacgacg cccagggtct tgccctggag ctcagtgccc aggatcttgc ggtcccagcc
     3541 gccggacttg gtggagacgt cggcctgggg gaccttgcgc gccaaggcga ggagcagcgc
     3601 ccaggtgtgc tcggtcgccg acatcaggtt cgccgtcggg gcgttgacca ccaggatgcc
     3661 gaggtcggtg gccgcggcga cgtcgacgtt gtccaccccg atgccggccc ggcccaccac
     3721 cttgaggcgc ttgccggcgc gcaggaggtc cgccgtcacc ttggtggcgc tgcgcaccac
     3781 cagggcgtcg aagccctcca cgatctcggg caggcgcggg cgctcctcct tctggagcag
     3841 gcggacctcg gcgcccgagg cgcggagcat ctcgagcccc gaagggtcca gcgagtcggc
     3901 gatcaggatg cggtgcatgg tcaggccgcc gccagctcgc ccagcacctc gtcgatctgg
     3961 gcgaggagcg cctccaggtg gttctcccgc acctcgccca tgtggccgat gcggaaggtc
     4021 gtcggcttcc agtcaccgta gccgccgccg atggtccagc cgcggtcggc caggcgcttg
     4081 acgatggcct gcgggtcgac cccggccggc ggatggaggc agctcaccgt ccacgagcgc
     4141 ccctcggcgc ccgacgcgta cgagcagccg cgggcggcgg cccactcctc ggtgcgctgc
     4201 tccaggcggc ggtggcgctc ccagcgcgcc tccatcccct cggcgaggat gcggtcgagc
     4261 tgcacgtcca gagcgtagag cagcgggatg cccggcgtgg tgatgaagcc cccctcccgg
     4321 tgcttgtcgc ggtagcgcag caggtcggtg tagaagccgc ggtgcggcac cttggcggca
     4381 cgctcggccg cccggtcgga gagggtgaag acggtgaggc ccggcggcac ggcgatggcc
     4441 ttctgggtgc cggcgagcac cacgtccagg ccccaggcgt cggtctcgac cggcgcgccg
     4501 cccagcgacg agacggtgtc caccaggatc agcgcgtcgc tctcctcgcg gatgacgcgg
     4561 gcgacgtcgg cgatcgggct catgacgccg gtcgaggttt cgttatggac catggtgacc
     4621 gcgtcgtact tcttgcgccg cagagcggca cggacgacct ccggatccat cggctgcccc
     4681 cagggcaccg cgaccttgtc ggcgttcttg ccgagggact tggcgatgga gtgccagcgc
     4741 tcggagaagg cgccgcaggt gaggttcaag acatccgagg cggcgcagga gaccacggcg
     4801 ctctccatga tcagggtcga gctgccagtg gcgaccatca cgtcgccctg ggtgcgcagc
     4861 agcgcgggca ggcgctcgga cagatgcagg tagaacttct tgaagccggc ggaccggtgc
     4921 cccgccatgg gcttggtcat cgcttggcgg gcatcctcgg ggacataagt cgggccgggg
     4981 aggaagaact ggatctgctc gttcacgaag acctcgaatc ggatggagag ttggatcgat
     5041 gggctgaagt acgaccacgg cgcctatgcc gcattgtggc aaaaccggtc gggggtggca
     5101 agcggccctc ccctacccgg gcctcaccag ggacggtgct tccgcagcgc cgggacgagc
     5161 cacccggcgt ctctgttgcc gcagtcccaa tttgggacta aaatccgccc atgcgtgtga
     5221 tctctctctc gacgctcaag gctttctggg aggggaaccc tgagtacgcc gactcgagag
     5281 agcccgtgct tgcctggtac cggcatgtcc tcagagccga ttgggactca cccgccgctg
     5341 tcaaggaaga gttccggcat gcgagcatcc tcagggatgg gcgggtggtg ttcaacatcg
     5401 ccgggaacaa gtaccggctg gtggtctgga tcaactacag atacagagtg gtgtacatcc
     5461 ggtttttggg aacccactcg cagtatgacc agatcgatgc acaaacgatt tgaggctgga
     5521 aggaggacag atggacatca agccgatcag gaccgaacag gactatcgag cgaccctcca
     5581 ggagatcgag tcgctgatga acgccgagcc ggggtcaccc gagggtgagc gcctcgacat
     5641 cttggttacc ctgctcgaag cgtacgagca gaagcatttc cccatggatc tgcccgatcc
     5701 ggtagaggcc atcaagttcg tcatggatca acgtgggctc acggtgaagg atctcgagcc
     5761 gatgatcggc cgccccaacc gcgtctatga ggtgctcagc cacaagcgcc ctctgaccct
     5821 caagatgatc tggagactgc acaaggatct cggcatcccg gcggagtgct tgatcaagca
     5881 gccggaggag caccgggcgg cgtagccggg gcggccggaa agacggtcaa gcccaaggcc
     5941 gtggcttgcg cagcgccggg acgagccggc ccgtctccag ccgcaccgcc gcctggccgg
     6001 gcaccgtcac ctcccactcc tcccccaccc accgcagcag cggcgcctcc aactcgcccc
     6061 gcaccagcac gagggcggtt ccccggaagt gctcgcgcag gaggtcttgc aggaccggcg
     6121 cgccgcgcag gggcttgacc acgacgctcc ggtcgccgcc cacggccacg gcgcggagga
     6181 caccggcggc ggcggcggtc tccaggtcgc cggccatcgg gggcggcggc gcgaggtcga
     6241 gccagccgac gcggagcccc tcggcgcggg ccgcctctat cagcggggcg aagcgctccg
     6301 gcccctcctc cacccgcagc aggtgggggg acttcacgcg ccgtcctcgt agaggaggat
     6361 gtcctggtac tcctcccgga acgccgcctc gtcggcggcc cagcccgcga tccagccggt
     6421 gagggctttc tcgtcgccgg tgtcgatggc ctcccggcac tcggggccgc cggtgaggag
     6481 gtcgatggcc ggccggtcgg agacgaactc gtagggggcc tcgcgccagg cgaagcggtc
     6541 cgggtccaac tggcggacgg tgaagaggag ctcgacgccg gtacggtagg gccgcagggc
     6601 cgcggcgtcg gtcaccacca gctctacgcc gccgcagact tggccgcggt gcttctggaa
     6661 ctgcggcttg aagtaggtgg ggaggaaggc gatcccgggc aggccgcgcg ccgccagacg
     6721 gtccgccagc gccaccgggt cgaggccggg cgcgccgacg agttggaatg gccgggtggt
     6781 cccccgcccc tccgacagct ccgtcgcctc caccaggcag ccccccggat agatcagggc
     6841 ggtgaggagc gtcggcatgt tgggcgacgg cgccacccag gggaggccgg tcgcctccca
     6901 gggcatgtcg cggcgccacc cctccatggc ccagacctcc aaaccctcct tccaaccgcg
     6961 ccgccgcgcc tccaggcgga ggatctcgcc caaggtcaag ccgtggcgca ccggcaggag
     7021 gaaggcgctg acgaacgact catggccggg acgcgggcga tccccctcga tgacgccgcc
     7081 gatcgggttg ggccggtcca gcacccagac ctcgcagccg gcggcgaggg ccgcctccgc
     7141 cgcccagacc gcggtggccg cataggtgta gtagcgcgag cccacgtcct ggaggtcgat
     7201 caccagcagg tcgaggccgt cgaaggcgca ggggcagggc ttcagcgact cctcgcggtc
     7261 gccgtagagg gagacgatgg gcacgccggt ccacgggtcg cgctcgttct ccgacgccac
     7321 catgtcctgc tcgacgccgt agtagccgtg ctccgggccg aagagggcgc ggggtgcctc
     7381 cccgtctgcg gacagcgccg ccagcgcacg gtggatcggc cgcggctcgc gggtgatcga
     7441 ggcgccgtgc gcgagcagcc cgtagcgccg gccggcgaga cgggaaggat cggcgagcag
     7501 gcggtcgagg ccggtgcgca tcacgctccc ccctgcctgc ggtacgagcc gctcttgccc
     7561 ccggacttgg cctccagccg gaggtcggtg atgacggcgc cgcgctcctg cgccttgacc
     7621 atgtcgtaga gggcgagcgc cgcggtcgag caggcgagca tcgcctccat ctcggcgccg
     7681 gtgcggccgg tgaccacgac gcggctgcgg atctccaccc cctcggggat cggctgcagc
     7741 tccacctcca cctgatcgag agggagcgga tgggcgagcg ggatcatctc cgcggtgcgc
     7801 ttcgccccct ggataccg
//
LOCUS       DASPJI010000224         3136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_414972, whole genome shotgun sequence.
ACCESSION   DASPJI010000224 DASPJI010000000
VERSION     DASPJI010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414972"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3136
                     /locus_tag="VEL74_05900"
     CDS             <1..>3136
                     /locus_tag="VEL74_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62093.1"
                     /translation="TEPLEIPLQKLFAAHAARTPDASAVAYGDELVTYGALKRRADRL
                     AGHLATLGVGPECVVAVAMERSVELIVAVLGTLTAGGAYLPLDPGYPRERLAFMLEDS
                     GAKVLLTQEALLGDLPAFRGDTVCLGAGDDAVAAAAPRSPRGPVELTAELASPAYVIY
                     TSGSTGRPKAVVVPHRGLANLAPVQARLFGVGPGSRVLQFASPSFDASVSEIAMSLGS
                     GALIRLAPRESLLPGADLERLLRERAITTVTLPPSALALLWDEALPDLACLVVAGEAC
                     PVDLAARWSAGRRFVNAYGPSEGTVCATAGEVGGRRLDIGRPIANVEVHLLDRHLQPV
                     PVSVPGELCLGGVGVARGYLGRPDLTAERFIPHALDGPRGESGGRLYRTGDLARWLPD
                     GSLQFLGRIDRQVKVRGFRIEPGEIEALLRRQPAVRDAVVEVRGDTQDGGRLVAYVVP
                     AGGAASVPAGALRDALRGALPEHMVPAAFVMLEALPLTPNGKVDRRALPAPNLAPAAS
                     YAAPRDPVEEVVADVWSELLAVERVGIHDDFFGLGGHSLLGARLMSRLFHVFGVELPL
                     RALFEFPTVAGLAAQLAAALRQGAGSETPPIERVEGEAEAPLSFAQQRLWWIHQLEPE
                     SPAYNLPVALRLHGRFIPQLLEAALGEIVRRHEVLRTTFPAVEGRPVQRISPPRPLRS
                     PLVDLSGLPGPRAEAAAAELAGAEALRAFDLSSGPLLRVALLRLGGDDHVLLFTLHHI
                     VSDGWSTGVLVREAGELYRAFLAGRPSPLPPLPIQYADFARWQRRWLQGEVLSGQLAY
                     WTSRLAGAPSVLELPADRPRPAVLSGRGGVREILLPADLTAALAALGRREGATLFMTL
                     LAAFYLLLSRYTGRIDLVVGTDVANRSRAETEDLIGFFINQLVLRADLAGDPPFRELL
                     GRVREMALEAYARQDLPFDRLVEELRPERDLSRQPVFQVFFTLQNLPRVPLELPGLTS
                     SLLAAGGGTAKFDLSVGVKEEPAGLRVSAEYSSDLFDAATIDRLLGHFAALLGSAAAA
                     PGTRLSGLEILPAAE"
BASE COUNT          354 a         1127 c         1167 g          488 t
ORIGIN      
        1 cacggagccg ctggaaatcc cactgcaaaa gctcttcgcg gcccacgcgg cacgaacgcc
       61 cgacgcctcg gcggttgcct acggcgacga actggtcacc tatggcgcgc tcaagcgccg
      121 ggccgaccgg ctcgccggcc atctcgccac gctgggtgtc ggccccgagt gtgtggtggc
      181 ggtggcgatg gagcgctcag tggagctgat cgtggcggtg ctcggcacac tcacggccgg
      241 cggcgcctac ctgcccctgg atcctggcta cccccgggaa cgcctggcct tcatgctcga
      301 agactcgggg gcgaaggtgc tcctcaccca ggaggctctg ctcggagacc tgcctgcctt
      361 ccggggggac acggtctgcc taggggccgg cgacgacgcc gtcgcggctg cggcgccacg
      421 aagcccgcgc ggccctgtag agctgacggc ggagctggcg agcccggcct acgtgatcta
      481 cacctcgggc tccaccggcc ggcccaaggc ggtggtcgtg ccgcaccgcg gcctggccaa
      541 cctcgccccg gtgcaggcgc ggctctttgg cgtcggtcca gggagccggg tgctccagtt
      601 cgcctcgccg agcttcgacg cctcggtatc cgagatcgct atgtcgctgg gctccggcgc
      661 gctgatccgc ctcgcgccgc gcgagtccct cctccccggc gcggacctgg agcgcttgct
      721 acgcgagcgg gcgatcacca cggtcaccct tccgccgtcg gcgctggctt tgctgtggga
      781 cgaggcgttg ccggatctcg cctgcctggt cgtggcgggg gaggcctgtc cggtcgatct
      841 cgccgcccgc tggtccgccg gccggcggtt cgttaacgcc tacggtccca gcgaggggac
      901 ggtctgcgcc acggcgggcg aggtcggcgg ccggcggctc gacatcggcc ggcccatcgc
      961 caacgtcgag gtccacctgc tggaccgcca cctgcagccg gtcccggtga gcgtgccggg
     1021 ggagctctgc ctcggcggtg tcggagtggc gcggggctac ctcggccggc cggacctgac
     1081 cgcggagagg ttcatcccgc acgccctgga cgggccgcgg ggggagtccg gcggccgcct
     1141 gtaccgcacc ggtgatctgg cacgctggct gcctgacggc agtctccagt tcctgggccg
     1201 catcgaccgc caggtcaagg tgcgcggctt ccgtatcgag cccggggaga tcgaggcgct
     1261 gctgcgccga caacccgccg tccgcgacgc ggtggtcgag gtgcgcggcg acacgcagga
     1321 cggtggccgg ctggtcgcct atgtggtccc ggccggcggc gcggcgtccg ttccggccgg
     1381 cgcgctgcgc gacgccctgc gcggcgccct tcccgagcac atggtgccag cggccttcgt
     1441 aatgctcgag gcgctgcccc tgacgcccaa cggcaaggtg gaccgccgcg ccctgccggc
     1501 tcccaacctg gcgccggcgg cgtcctacgc ggcgccgcgc gatccggtcg aagaggtggt
     1561 cgcggacgtc tggagcgagc tgctggccgt cgagcgtgtg ggcatccacg acgatttctt
     1621 cggcctgggc ggtcactcgc tactgggcgc gcgcctgatg tcccggctgt tccacgtctt
     1681 cggcgtggag ctgccgctgc gggcgctgtt cgagttcccc accgtggccg gactggcggc
     1741 gcaattggcg gcggccctga ggcagggggc agggagcgag acgccaccca tcgagcgggt
     1801 agagggggag gcggaggcgc cgctgtcgtt tgcccagcag cggctgtggt ggattcacca
     1861 gctcgagcct gagagcccgg cgtacaacct gcctgtggcc ctgcggctgc acggccggtt
     1921 catcccgcag ctgctggagg ctgccctggg tgagatcgtg cggcggcacg aggttctgcg
     1981 cacgaccttc cccgccgtcg aggggaggcc ggtgcagcgc atctcccctc cccgacccct
     2041 gcgctccccc ctggtggatc tgagcggcct gcccggaccg cgggccgaag cggcggccgc
     2101 cgagctggct ggcgccgagg cgctgcgggc gttcgatctg agctcggggc cgctcctgcg
     2161 ggtggcgctc ctgcgcctgg gaggggacga ccacgtgctg ctcttcacgc tgcaccacat
     2221 cgtcagcgac ggctggtcga cgggcgtgct ggtgcgcgag gcaggggagc tctaccgggc
     2281 cttcctcgcc ggccggccgt cgccgctccc ccctcttccc atccagtacg cggactttgc
     2341 ccgctggcaa aggcgctggt tgcaggggga ggtgctgagc gggcagctgg cctactggac
     2401 gagtcgcctg gccggtgctc cgtcggtgct ggagctgccc gccgaccggc cgcgtccggc
     2461 cgtgctcagc ggccgcggcg gcgtccggga gatcctcctg ccggcggact tgaccgccgc
     2521 cctcgcggcg ctcggccgtc gtgaaggggc caccctgttc atgaccctgc tcgcggcgtt
     2581 ctacctcctg ctgtcgaggt acacgggccg catcgacctc gtggtgggga cggacgtggc
     2641 caaccgctcc cgggcggaga cggaagacct gatcggcttc ttcatcaacc agctcgtgct
     2701 gcgcgccgat ctggccgggg acccgccgtt ccgcgagctc ctggggcggg tgcgggagat
     2761 ggcgctggag gcctatgcgc gtcaggacct gccgttcgac aggctggtgg aggagctgcg
     2821 gcccgagcgc gacctctccc ggcagccggt gttccaggtg ttcttcacgc tgcagaacct
     2881 gccccgggtc cccctggagc tgcctgggct gacctcgagc ctcttggcag ccggtggcgg
     2941 cacggcgaaa ttcgatctgt cggtgggggt gaaggaagag ccggccggcc tgcgggtgag
     3001 cgccgagtac agctccgatc tgttcgacgc cgcgaccatc gaccgtctgc tcgggcactt
     3061 cgcggcgctg ctcggcagcg ccgcggcggc tcccgggacc cggctctccg gcctcgagat
     3121 cctgccggcg gcggag
//
LOCUS       DASPJI010000225         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_415092, whole genome shotgun sequence.
ACCESSION   DASPJI010000225 DASPJI010000000
VERSION     DASPJI010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415092"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /locus_tag="VEL74_05905"
     CDS             complement(<1..1473)
                     /locus_tag="VEL74_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG62094.1"
                     /translation="MDLLWKDIRFGLRTLVRNPATTGVALLALALGIGANTAIFSVVS
                     GVLLEPLPFSEPDELVLVWETHTGRGMPRVSVSPPNFNDWRQQARSFEGLAASERAQF
                     TLTGGERPELLAGASVSAGFFTLLGAPMVVGRDFTAEEDRPGAGRVAILSEGLWRRLF
                     ASDPQILGRTLRLSGEPYTVVGVAPAVVDLPNDSQLWVPLASEFLPEDRGAHSVRVLG
                     RLKPGVSLERAQAEMSAIAARLERQYPENEGWGALVMPLRESLVQNIRPILLILLGAV
                     SFVLLIACANVANLLLARVAAREREIAVRTALGAGRWRLMRQMLTESAVLFLGGGLLG
                     LLLAYWGTRALVAMSPEGVIPRAAGIGLDGRVLLFTFGVSLLTGLLFGLVPALHATGG
                     PLQPSLKEGGRGQSGGLRGRWVRNALVLAEVSIALMLLVGAGLLIQSFSRLRSVDPGM
                     RTDGVLTAEIAIAETKYPEPAQQTIFFRQLLERLQAMPGVE"
     gene            complement(1535..>2539)
                     /locus_tag="VEL74_05910"
     CDS             complement(1535..>2539)
                     /locus_tag="VEL74_05910"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HYG62095.1"
                     /translation="ANNHPGVLACFHGLGIGFECVSAGELDHILGLFPGLPGERILFT
                     PNFAPREEYRRALELGAHVTVDSLHPLRDWPEVFAGRDVFLRLDPGHGRGHHAHVRTA
                     GAQSKFGISPEEVEQAAALAVEHGARVVGLHAHAGSGIRVAEAWAETARFLAAAAPLF
                     PHLRALDLGGGLGVPERPGQPPLDLDVLAGTLRAFRREHPGLELWLEPGRYLVAQAGV
                     LLARVVQIKRKGEATYVGLETGMNSLIRPALYGSYHPIYNLSRLGEAPSQTVHVVGPI
                     CETGDVLGRSRRIAPAAEGDVLLIANAGAYGRVMSSRYNLREPAAERLLPPRPWTEKP
                     "
BASE COUNT          387 a          929 c          924 g          299 t
ORIGIN      
        1 ctccaccccg ggcatggcct ggaggcgctc caggagctgc ctgaagaaga tcgtctgctg
       61 cgccggctcg gggtacttgg tctcggcaat ggcgatctcc gccgtcagca ccccgtccgt
      121 gcgcatcccc gggtccaccg aacggaggcg cgagaagctc tggatcagca ggcccgcccc
      181 gaccagcagc atcagggcga tggatacctc cgcgagcacc agggcgttgc gtacccagcg
      241 gccgcgcagg ccgcccgact gtcctcgtcc cccctccttg agcgacggct gcagcggccc
      301 gccggtggcg tggagcgccg gcaccaagcc gaacagcagg ccggtgagca gcgagacgcc
      361 gaaggtgaag agcagcacgc ggccgtccag gccgatgccg gcggcgcgcg ggatcacccc
      421 ctccgggctc atcgccacca gcgcccgggt gccccagtag gcgagcagca gcccgaggag
      481 gccgccgccg aggaacagca ccgcgctctc ggtgagcatc tggcgcatca gccgccagcg
      541 ccccgccccg agggcggtgc gcaccgcgat ctcgcgctcc cgcgccgcca cccgcgccag
      601 cagcaggttg gcgacgttgg cgcaggcgat gagcagcacg aaggagaccg cgcccaacag
      661 gatcagcagg atcggccgga tgttctgcac gaggctctcg cgcagcggca tcaccagcgc
      721 cccccaacct tcgttctcag ggtactgccg ctccaggcga gcggcgatgg ccgacatctc
      781 ggcctgggcg cgctccaggc tcacccccgg cttgagccgc ccgagcacgc gcaccgagtg
      841 ggcgccccgg tcctccggca ggaactcgga ggccagcggg acccagagct ggctgtcgtt
      901 ggggaggtcc acaacggcgg gggccacgcc taccaccgtg tagggctcgc cgctgagccg
      961 gagggtcctg cccaggatct ggggatcaga ggcgaacagg cgccgccaga gcccttccga
     1021 gaggatggcg acgcggcccg cccccggccg gtcctcctcg gcggtgaagt cgcgtcccac
     1081 caccatggga gccccgagga gggtgaagaa gccggcggag accgacgcgc cggccagcag
     1141 ctcgggccgc tccccaccgg tgagggtgaa ctgagcccgt tcgctcgccg ccagcccctc
     1201 gaagctgcgg gcctgctgcc gccagtcgtt gaagttgggg ggagagaccg agacccgcgg
     1261 catgccccgg ccggtgtggg tctcccacac cagcaccagc tcgtcgggct cggagaacgg
     1321 cagcggctcg aggagcacgc cgctcactac gctgaagatc gccgtgttgg cgccgatgcc
     1381 gagcgccagg gcgagcaggg ccacgccggt ggtcgccggg ttcctgacca gggtccgcag
     1441 gccgaagcgg atatctttcc agagtaagtc caagctcaat acctccgccc gcggaccgaa
     1501 aggtgcctac gccgtgatct acggagaaga gggctcaggg tttctccgtc cagggacgcg
     1561 gcgggagcag gcgctcggcc gcgggctcgc gcaggttgta gcgcgagctc atcacccggc
     1621 cgtaggcgcc ggcgttggcg atgagcagca cgtccccctc ggcggccggg gcgatgcggc
     1681 gcgagcggcc gaggacgtcg ccggtctcgc agatcggccc gaccacgtgc acggtctggg
     1741 acggcgcctc gccgagccgc gacaggttgt agatcggatg ataggagcca tagagcgccg
     1801 ggcggatcag cgagttcatg ccggtctcga gcccgacgta cgtcgcctcc cccttgcgct
     1861 tgatctggac cacgcgggcg agcaggacgc cggcctgggc caccaggtag cgccccggct
     1921 cgagccacag ctcgaggccc gggtgctcgc gccggaaggc gcgcagcgtg ccggcgagga
     1981 cgtccaggtc gaggggcggc tggcccgggc gctcgggcac gcccagcccg ccgcccaggt
     2041 ccaacgctct gaggtggggg aagagcggcg ccgcggcggc caggaagcgg gcggtctcgg
     2101 cccaggcctc ggcgacgcgg atgccgctgc cggcgtgggc gtggagcccc accacccgcg
     2161 cgccgtgctc caccgccagg gccgccgcct gctccacctc ctcgggcgag atgccgaact
     2221 tggactgcgc gccggcggtg cgcacgtggg cgtggtggcc gcggccgtgc cccgggtcga
     2281 ggcgcaggaa gacgtcgcgc ccggcgaaca cctccggcca gtcgcgcagc ggatggaggc
     2341 tgtccaccgt gacatgtgcc cccagctcca gcgcccgccg gtactcctcc cgcggcgcga
     2401 agttgggggt gaacaggatg cgctcccccg gcagcccggg gaacagcccg aggatgtggt
     2461 ccagctcccc cgccgacacg cactcgaagc cgatccccag gccatggaag caggccagga
     2521 ctccgggatg gttgttggc
//
LOCUS       DASPJI010000226        15503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_527342, whole genome shotgun sequence.
ACCESSION   DASPJI010000226 DASPJI010000000
VERSION     DASPJI010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15503)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15503
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527342"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..839
                     /locus_tag="VEL74_05915"
     CDS             81..839
                     /locus_tag="VEL74_05915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG62096.1"
                     /translation="MKYRDAARFYEAYRDRYPAELFVWLEEVLGERVRGRVLDLAAGT
                     GFLSRPFAAGGAEVVAVDEDPGMMAEGKRAGAGRVHWVRARSPRLPLRPGSFDAALIG
                     NAVHWLAAELPAADLAALLRPGGCLAMVTRSHPLAGPEPWKEPVRQVLAELLPERLTR
                     FAVEVEPNLGRHEAYLAPPFSLVADRPLSATFSRPVRDYAGFLLSTSWFEAVRSGELL
                     AELHRRMDPVVRPYLENGEVRETVRYRCTVARVP"
     gene            1195..1476
                     /locus_tag="VEL74_05920"
     CDS             1195..1476
                     /locus_tag="VEL74_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62097.1"
                     /translation="MDSNCTPSLSEVSRQNRGTYGNGSMFSCSHHRVDWVTKYDNNQC
                     NTNSAYWYVSSCEDTLDGGKSGWFPDCCNGYGPGGTLDSTYTCNHQHSC"
     gene            complement(1536..1874)
                     /locus_tag="VEL74_05925"
     CDS             complement(1536..1874)
                     /locus_tag="VEL74_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor
                     domain-containing protein"
                     /protein_id="HYG62098.1"
                     /translation="MAKRRIQLLGLSVALVTALLLPSCATKGDPDGQVCGGIQGLPCP
                     DGNFCDLPAGQCQSADLQGVCVPVRDACTRDYRPVCGCDGKTYSNECERIRAGVQKNH
                     DGECRTQTGS"
     gene            complement(2566..3159)
                     /locus_tag="VEL74_05930"
     CDS             complement(2566..3159)
                     /locus_tag="VEL74_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62099.1"
                     /translation="MNGCTEVTTSPAQSLPNAFTSSFLGRLAERDEPPTAGDADVAGP
                     WRVESIPGQGFGLFRVGESLGRGFAPTAVYPSRWLALLMAGILPGTGKDPLLLLSKQP
                     DAHGVYPVALAGGSDGGDGGGGDDPGGRVPAGFLQLFDEKAVDALNVVIHLLQAPESL
                     AFVLEAAGGVTLERCGAILDERLQEAGAEGSGGAAAG"
     gene            3510..4622
                     /locus_tag="VEL74_05935"
     CDS             3510..4622
                     /locus_tag="VEL74_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62100.1"
                     /translation="MTSIQARKAFVIGPIGDRDGDLGTIERLTWEDAVQVFEEIIEPA
                     LQANGFIAIRADKIARSGEIPEQIFRHLRDRELVIADLTAANPNVMYELGLRHTTGKL
                     TIQIGERGRLPFDVAAIRTILFRRTEAGFVRARRDLTEAIEEGLAEGGDPVTATRIWF
                     EQPAGQSSAPVKNSSSPAVAEGEEPEEEPGFLESLADAQEGWTAITSDLELVTSITQE
                     IGAIMREGSSQVQKVNQQGGDSSAKLFVANRVAHQLDEPASRMEVAVGAYRLSMQRVD
                     AGMSYIIQRLRDEPELRAGAERFIENTTKLAEVSAVSLAQAEGYRDSLKPLGEASRSL
                     RRVANRISRSLDQFIEASQSIRRWREGLEGLQGPVA"
     gene            complement(4786..4992)
                     /locus_tag="VEL74_05940"
     CDS             complement(4786..4992)
                     /locus_tag="VEL74_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HYG62101.1"
                     /translation="MAANLALDDSLIEEAVKVGNHRTKKEAVTAALLEYVQARKRLGI
                     LAWAGKVDYDGDYDPKRLRGRKSL"
     gene            complement(5016..5306)
                     /locus_tag="VEL74_05945"
     CDS             complement(5016..5306)
                     /locus_tag="VEL74_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG62102.1"
                     /translation="MSDLTKYVRARAARDPKFAQGFEEGYENFKIGALLRQARERAGL
                     TQEEVARRLETKKSAISRMETSAGSIRLSTLERYARAIGWELSLELRPPHQE"
     gene            complement(5290..5655)
                     /locus_tag="VEL74_05950"
     CDS             complement(5290..5655)
                     /locus_tag="VEL74_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002790475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYG62103.1"
                     /translation="MRAIHFYRTKAGACPVEEFLDSLTGQQAQKVLWVLRLVEELEMV
                     PAQYLKKLVGTEGLWEIRAQSGGDTFRLLGFFEGHRLFVLTSGFAKKTEKIPRQEIAL
                     AERRREDYLRRRHEHERSH"
     gene            5926..6315
                     /locus_tag="VEL74_05955"
     CDS             5926..6315
                     /locus_tag="VEL74_05955"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HYG62104.1"
                     /translation="MVPVLPLLFAGIGLLLIGLSVPLIRRRVKPNALYGLRVPATFAD
                     EWVWYEANARSGRDLLLLGTLQIVAASLPLWIPGLSEAAYALTNAGLVGIGALLLCIV
                     GWLRANRLLREQEAGRSLSTGRPRDGG"
     gene            complement(6316..7218)
                     /locus_tag="VEL74_05960"
     CDS             complement(6316..7218)
                     /locus_tag="VEL74_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HYG62105.1"
                     /translation="MSIRSVKSLSQSTPTTEGAGVRLRRAFGFGETGDFDPFLLLDDF
                     RNDRPADYRAGFPWHPHRGIETITYVLSGTVEHRDSLGNMGALGAGDVQWMTAGRGIV
                     HQEMPQGDESGRMHGFQLWANLPSSLKMTAPRYQDVKSAEIPEVVDDDGTRARILCGS
                     FWGKTGPVEGIAAEPSYIDIWVPPGRRKVLPVETARNAFAYVFAGSGRFANASPPRGV
                     LMESVDGTETLSWEAAGDRSLVVFDRGDEVVVQAGEEGIRFLLASGKPLEEPVAWHGP
                     IVMNTQAELHRAIRQLQDGSFLEG"
     gene            7354..7644
                     /locus_tag="VEL74_05965"
     CDS             7354..7644
                     /locus_tag="VEL74_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYG62106.1"
                     /translation="MLEVRQTVEFEEWFDGLRDRQARARIDVRLRRLSLGNPGDVKPV
                     GEGVSELRIDYGPGYRVYFIRQGQSVVVLLAGGDKRTQAADVARAIELAHHL"
     gene            7658..7945
                     /locus_tag="VEL74_05970"
     CDS             7658..7945
                     /locus_tag="VEL74_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011153707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HYG62107.1"
                     /translation="MAKTKTSPWDPANHLKTEEDMAAYLEAALEDGDPALVAAALGDI
                     ARAKGMTQIAREAGLGRESLYKSLSPEGNPELATVLKVVKAIGLRLHASTL"
     gene            complement(8018..9016)
                     /gene="add"
                     /locus_tag="VEL74_05975"
     CDS             complement(8018..9016)
                     /gene="add"
                     /locus_tag="VEL74_05975"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006808980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HYG62108.1"
                     /translation="MRNGYPLVELHRHLDGNVRLETVLDLARQHGIGLPGRTLEELRP
                     HVQVAERESDLMAFIAKFAVLRRVMVDYDAVRRIVRENLEDAARERIDYIELRFSPRF
                     MAEEHGLDTLAVADAACDALEEARGVIPVRAKLIVIISRHYGPEAGFLELEAAIRCRD
                     RGVVALDLAGDEAGFPGDLFVEHFRRAREAGLHVIAHAGEAAGAGSVRQAVLALGAER
                     IGHGVRSIEDPAVLALLEERRIPLEVCPTSNLHTSTFPDYRSHPLPFFLRRGLLATLN
                     TDDPSISGIDLAHEYRIAQYELGLDEVDLRRLQENALHAAFLTPEERAELEGMASA"
     gene            complement(9029..9751)
                     /gene="rsmI"
                     /locus_tag="VEL74_05980"
     CDS             complement(9029..9751)
                     /gene="rsmI"
                     /locus_tag="VEL74_05980"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HYG62109.1"
                     /translation="MAGKLLVVATPIGHLDDLSPRARAAFEEADLIACEDTRHTGRLL
                     AHIGVKKPLVSLHEHNERQRLPRLLADLEEGKVIAVASDAGTPLLSDPGYVLVREAAA
                     RGVRIEPIPGPSAVLTALVASALPPYPFTFAGFPPPKSGKRRSFYKRWAGLGHTLVIF
                     ESPHRLLASLEDALAELGDRPAAVARELTKLHEEVLRGLLSELLETLRKRTALKGEFV
                     VMVAGSDAVPETSEAPEDNEEP"
     gene            complement(9751..10587)
                     /locus_tag="VEL74_05985"
     CDS             complement(9751..10587)
                     /locus_tag="VEL74_05985"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HYG62110.1"
                     /translation="MIRREQVAARLELNAELASAVLTSFIRDAVETSRTDGVVIGLSG
                     GVDSSLAAALAVRALGPERVHGFLLPYRTSSPESVADAHAVAGHLGVPHREIDISPMV
                     DAYFETAEPDADPGRRGNKMARERMTILFDQAKKLGCLVLGTSNKTEILLGYSTVFGD
                     NASSLNPLGDLYKCQVWQLSRHLGLPGQVIDKKPSADLWPGQTDEGDLGFSYATADEV
                     LHLLFDLGLLPHEVVARGYDEAAVERIVRLEKQFRFKRRLMLIARLSGSAVNLDQEIP
                     RD"
     gene            complement(10574..11416)
                     /locus_tag="VEL74_05990"
     CDS             complement(10574..11416)
                     /locus_tag="VEL74_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HYG62111.1"
                     /translation="MAEAPSILRIGLAQIDCALGDVAANAERHLAWIERAREEKVDLL
                     VFPELSLTGYRLLHLTSRVALPADSPVLARLAEAAGPMAVVLGFVEEDRRGILHNSAA
                     LLQHGSPVWVHRKLYLPTYGIFQEERFLGAGKRLALAPLPSGNAGLLVCEDLWYPDLA
                     RRLATAGARLIVVISAGPGRVGSGQLPASQENWELLTRSTALVNTCWVAYCNRVGWEE
                     GSFYPGGSHIVRPGGELTERAPLLEEHLLVTEIDLRDADRLRWSLPLLADIRDDIEGP
                     DDPA"
     gene            11447..12223
                     /locus_tag="VEL74_05995"
     CDS             11447..12223
                     /locus_tag="VEL74_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62112.1"
                     /translation="MRTVPLFRTLLALVCLTAAGTAGAGAQSPKPPAPTTSAPQGQAV
                     LKDRIIAVVDEDPILLSDLERVIALNLIRPREGEAGAAYRRRVLGQMIEQRLRLHEID
                     RFGLEQVPVDEIERRVSEVRASFPDEAAFQRTLRQQGLTLPALRQLLTRQLVVMTYVD
                     ERLGPRVFIDSEDIRAYYDRTLAPEMQRRGQPVPALEDVREEIRTVLREQRLNDEIRR
                     WTTELREEADIAVYLDTPQGALPPVVKTLDGSSGKKSPRP"
     gene            complement(12230..14452)
                     /locus_tag="VEL74_06000"
     CDS             complement(12230..14452)
                     /locus_tag="VEL74_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HYG62113.1"
                     /translation="MLGTLTLFLAVAATDPRPALVELQIEGRPRSALDVVEQESRERP
                     DASRRIGLDYLRGHLLELLGDPRGAGEAFADALAVPELRFYSRYRMALEQERQRHPEV
                     AAGLIATALAQASPSSPLLPEAVQLLARTLEKGGDCRLLRPLKPDTMPARERRLLLLS
                     QADCAQRSELREFARSLLTGLLEENVRDDTARAAADRLAGLVSESEAGRLPMLLGMSF
                     YRHHDLDRALQYLRRAIGKRGLSQRDSFEVLQALGQSQLLQGRADQAAGTFGSLAVRA
                     RTPEQRARALHQQGRAHELQGRWQKAVESFGAAYQASPASSEWAPASLFAALRLDWRR
                     GREGPALATYQVLAGRREWRHTIVRASLFLAASDLVRGRSDRVGGWLARTAGDRGSRI
                     ESAYWRGRLAELQGEGTAAVAAYLEILRADALHPLAQGAHRRLAAEPLARVTLAEGRR
                     RANLGGVDNARDAWLLLGDGDPAGRKAGFQLRQLLLADARTAPFLRMPIVPVGQWPLW
                     SSDLRRPEEMLLALGLWHEGGPAVATHFPVRNPSLALTGSRRLASAGELPRSIQLAEE
                     LRRRTPERVLLRVQPRDFRQALHPPAYRELLMTQGRTRGVDPDLLAAVIRNESRFDPL
                     SLTESGERGLTHLSPARARVLARQAGLGSFAPEDLYRPEVAVALGATHLAGLVQAFGG
                     ATHVAVAAYDAGEPQAVVWRSYCNSLEPEEFFTKVASDETRRYLQQVLSGWAEYQALY
                     "
     gene            complement(14507..>15503)
                     /gene="murA"
                     /locus_tag="VEL74_06005"
     CDS             complement(14507..>15503)
                     /gene="murA"
                     /locus_tag="VEL74_06005"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HYG62114.1"
                     /translation="MRASVLVLGPLLAKLGHARVSLPGGCAIGVRPIDQHLKGLTALG
                     AEVRLDRGYVEATSPRLTGARFRFDMPTVTGTENVLMAAALARGTTVLENCALEPEVV
                     DLANLLNAMGARIHGAGESTITIEGVDGLGGSEHAIIPDRIEGGTYLVGAAMTGGDVL
                     LEQAVPADLAPLLDKLAEVGAKVEVLPEGVRVSADGNLQARSIETCPHPGFPTDLQAQ
                     YMALMTQAAGEATISETIFENRFQHAPELARMGADVRVDGGKAVIHGPTPLTGAGVMA
                     TDLRASACLVLAGVAAEGETVVDRIYHLDRGYEGMELKLQALGARVARWAIPSAA"
BASE COUNT         2399 a         5154 c         5487 g         2463 t
ORIGIN      
        1 ctaccggttc tgaggagcgg gggcggcggg gcatggcgac ctcttgcgtg aaaggctaga
       61 cctccccagt atacccctcg atgaaatacc gcgacgccgc ccgcttctac gaggcctatc
      121 gggaccgcta ccccgccgag ctgttcgtct ggctggagga ggtgctggga gaaagggttc
      181 gcgggcgcgt cctggacctg gccgccggca cgggcttcct gtcgcgcccc ttcgcggccg
      241 gcggtgccga agtggtggcg gtggacgagg acccgggcat gatggccgag gggaagcggg
      301 ccggcgccgg tcgcgtccat tgggtgcggg cgcggtcccc ccggctgccg ctccgtccgg
      361 gctcgttcga cgccgcgctg atcggcaacg ccgtccactg gctggccgcg gagctgccgg
      421 cggccgattt ggcagcgctg ctgcggccgg ggggctgcct cgcgatggtg acccggagcc
      481 accccctggc gggccccgag ccctggaagg agcccgtccg gcaggtgctg gcggagctcc
      541 tccccgagag gctcacccgc ttcgccgtgg aggtggagcc caacctcggc cggcacgagg
      601 cctacctcgc cccgcccttc tcgctggtgg cggaccggcc cctgagcgct accttctcgc
      661 gaccggtccg cgactacgcc ggcttccttc tctccacctc ctggttcgag gccgtccgct
      721 ccggcgagct gctcgccgag cttcaccgcc ggatggatcc ggtggtccgt ccctatctgg
      781 aaaacggcga ggttcgggag acggtccggt accggtgtac ggtggcgaga gtgccgtaga
      841 taacgggtga cggttcagct ctcccttcat gcaccgcagg aacagcacct cggggctgcc
      901 ctcgggcgcg ctctcctgac ggcgagcacc attaatttat ggaagaagct gaactaaagg
      961 aggcctgaca tgaggcatgt ttcccgctca ctgttctcca cccttctctc cgtgccgctt
     1021 gcccttctct tcgccaccgg cgcacgagcc cagatcgaat gctacgaatg tactccgtac
     1081 tcttcgtgtg ccgaatcgtg cttgcagtgc cagggggcct tgcatccgga aggctactgc
     1141 gacagttacc tgtacacgac gtgcggcgct gtcggcggct acacctacgg ctgcatggac
     1201 tcgaactgca cgccctcgct gagcgaggtc tcgcgccaga accgcggcac ctacggcaac
     1261 gggtccatgt tcagttgctc gcatcaccgg gtggattggg tcaccaagta cgacaacaat
     1321 cagtgcaaca ccaactccgc ctactggtac gtctcgtctt gcgaggacac gctcgacggt
     1381 ggcaagtcgg ggtggttccc ggactgctgc aacggctacg gtcctggcgg tacgctcgac
     1441 agcacgtaca cctgcaacca ccagcacagt tgctgatcag gaaggccggc cgggcattcg
     1501 cccggccggc cccgattctt gaggctgaga aatccctagc ttccggtctg cgtccggcac
     1561 tcgccgtcgt ggttcttctg gacaccggcg cggatgcgct cgcactcgtt ggagtaggtc
     1621 ttgccgtcgc agccgcagac aggcctgtag tcgcgggtgc aggcgtcgcg gaccgggacg
     1681 cagacgccct ggaggtcggc gctctggcac tggcccgccg ggaggtcgca gaaattgcca
     1741 tcggggcagg gcaggccctg gatgccgccg cagacctggc cgtccgggtc gcccttggtg
     1801 gcgcacgagg gaagcaggag ggccgtcacc agcgcgaccg acagccccag gagctggatt
     1861 ctacgcttcg ccatggcatt ctcctttcgg gttgttactt ctctgctgaa ggatgataga
     1921 cccaaaccgc ctcgcgaaaa cgtccaagcg cggcgctccc cccgctcggg cgccgcgccc
     1981 gaaccatctg tccatagatt gtcaaagaac cgatggtgag cgccacatta tgcgccgccc
     2041 actcccttgc aactcctgca tcatacatca cgaccggcca tagtgattct attgacattg
     2101 ctgcagcttt ctttgtaggc tctgtcctcg tcgggacgct ttccccggcc tgctgctctt
     2161 tgacaactga atcgtgacgg ggcgccgccg aggcccaggc cgcgcccccg aggatctctc
     2221 cgtccgacag ggggacaccg ggaggcggag gtccgatccc tccggctccc ggctcgtcat
     2281 ccccggcgaa acgttcgcca tacccgaggt agcgtcgtcc tccccgggag catggccgcc
     2341 gtacccgcgg ggcggggagc gtacccaggg gtagggacac cgtgcccgga ggcacgggct
     2401 ccgtaccttg ggtatggcct tcgtacccga ggggatggag gcgtcgccgc ggggatggtc
     2461 ttcgtctggc gggggatggt ggccgcgccg gcgtggtttt tacagggctg gaagggggcg
     2521 ccaccggcgg gccgagccca ccggtgacgc caatgagaga atgtcctacc cggcagccgc
     2581 cccgccggag ccctcggccc cggcctcctg cagccgctcg tcgaggatgg ccccgcaccg
     2641 ctcgagggtg accccacccg ccgcctccag cacgaacgcc agagactccg gagcctggag
     2701 cagatggatg acgacgttca gcgcgtcgac cgccttctca tcgaagagct gaaggaagcc
     2761 ggccggaacg cggcccccgg ggtcgtcgcc gccgcctcca tcaccgccgt cactgccgcc
     2821 ggcgagggcg acgggataga cgccgtgggc gtcgggctgc ttggagagga ggagcagcgg
     2881 gtccttcccg gtgccgggaa ggatgcccgc catgaggagc gccagccacc gggacgggta
     2941 caccgccgtc ggtgcgaagc cgcgacccag gctctcgccc acccggaaca ggccgaagcc
     3001 ctgacccgga atggactcga cacgccaagg cccggcgacg tcggcatcac ccgccgtggg
     3061 cggctcgtcc cgctcggcca gacggccgag gaaagagctg gtaaaggcgt tagggaggga
     3121 ttgggcgggg gaagtggtaa cttcagtaca gccgttcata agtcctcctt ggggactgtg
     3181 ggcggtcagg ctcggtggtg agttcgccgc tcacctccgg gccgtcagcg gaattgccgg
     3241 cccgcccttg ctgcgcttct acatctggat gcctctgaca cccctggagc gaccgtggtc
     3301 gtcttcgggg ggtgagggga gcatatgata ggcgtggagg tggttgcaag gggttcgcag
     3361 tcttcgcctc gcttcgcagc gttgcgtcga tcgaagaaac gggtgaacgg aatctcgtct
     3421 cgggttaaca gctaccctgg atggggagag aggacggcaa gccgcccctc atggacactg
     3481 acctcagaga ctgtgatatg gagaactgaa tgacgtctat tcaggcccgc aaggcttttg
     3541 tcattggtcc aatcggtgac cgggacggtg atctcggaac catcgagcgc ctcacatggg
     3601 aggatgccgt gcaggtcttc gaggagatca tcgaacctgc gctgcaggcg aacggcttca
     3661 ttgctattcg tgccgacaag attgcgcgct ccggtgagat cccggagcag atctttcggc
     3721 atttgcggga ccgcgagctt gtcatcgcgg accttactgc tgcaaatcca aatgtaatgt
     3781 acgaacttgg gctccggcac acgacaggca agctcacaat ccaaatagga gagcgtgggc
     3841 ggctcccgtt cgatgtcgcc gcgatccgga cgattctctt ccgtcgaact gaagccggct
     3901 ttgtccgtgc tcgccgagat ctcacggagg caattgagga gggtttggca gaaggtggtg
     3961 atccagtcac ggccacccgc atctggtttg agcagccggc aggtcagtcg tctgctccag
     4021 ttaaaaactc aagcagccct gcggttgccg aaggagagga gccggaggag gaacccgggt
     4081 ttttagagag tctagctgac gcgcaggagg gctggacggc aataaccagc gatctggaac
     4141 tagtaacttc gatcactcaa gagattggtg ccattatgcg agaaggaagc tcgcaagttc
     4201 aaaaagttaa tcaacaaggc ggagactctt cagcgaagct attcgtcgca aatcgagtcg
     4261 cacatcagtt ggatgagcct gcgagtcgaa tggaagtcgc ggtaggtgct taccggctct
     4321 caatgcagcg tgttgatgcc gggatgtcat atattatcca acggctccga gatgagccgg
     4381 agctccgagc tggcgccgaa aggttcatag aaaatacaac gaagctcgcc gaagtttcag
     4441 cggttagcct cgctcaagct gaaggttacc gcgactcctt gaagcccctt ggcgaggcct
     4501 cgagatccct ccgccgagtg gctaaccgta tctcccgtag cttggatcaa ttcatcgaag
     4561 catcacaatc gatacggcgg tggagagaag ggcttgaggg attacaaggt ccagtcgcct
     4621 gatgcctagc ctcattgatt gcaggtacct gttcttctga ggctaccagc tggcaccgcg
     4681 aagacgaact ctgccatccc tcgacgatgc tcttctccac cggaggaagc tggtgcgagc
     4741 gccgccgcaa cgcgagcgac tagatggtgg tgtcggcgag gacggtcaca gggacttgcg
     4801 cccacggagc cgcttgggat cgtagtcccc gtcatagtcg accttgccgg cccaagcaag
     4861 gattcccaac cgtttgcggg cctgcacgta ttcgaggaga gcggctgtga ccgcttcctt
     4921 cttcgtccga tggttgccga ccttgacagc ctcttcgatc agggagtcat cgagcgcgag
     4981 attcgcagcc accttatcgc ctcctcaacc agtctttact cttgatgagg aggccgaagc
     5041 tccagcgaca gctcccagcc gatggcccgg gcgtagcgct ccaatgtgga gagcctgatg
     5101 ctgccggcgc tggtctccat tcggctgatg gccgacttct tcgtctccag acgcctggcg
     5161 acctcctcct gagtcaggcc agctctctcg cgggcttgac gcaggagggc gccaatcttg
     5221 aagttctcgt acccttcctc gaaaccctgg gcgaatttgg ggtcgcgggc ggcacgcgct
     5281 cgtacgtact tagtgagatc gctcatgctc gtgcctcctt ctcaagtagt cctctcggcg
     5341 cctttcggcc aacgcaatct cttggcgggg aatcttttcg gtcttcttcg cgaagccact
     5401 ggtcaacacg aacaaccggt gtccctcgaa gaatccgagg agcctgaacg tgtctcctcc
     5461 gctctgagcc ctgatctccc acaggccttc ggtgccgacc agcttcttga ggtactgagc
     5521 gggaaccatc tcgagctctt cgaccagccg caagacccaa agaaccttct gggcctgctg
     5581 gccggtcagt gaatccagga actcctcaac gggacaggcg ccagcctttg tccggtagaa
     5641 gtggatggca cgcatcagga ggttaacact attgtgaacg agggtccatc ccagctcgcc
     5701 ggcagggcgc ccttcgcccc atccgggctc ggatgatctc cggcccccag aggcgcgaac
     5761 aggatctcgt ccagaggtag caacatccta tgaagagggc tggaggttcg tcaacccgcg
     5821 ttgaagtctt gcaggcccag caggcaagcg ccacccttcg tggggcccgt gcctgttcca
     5881 cggagccgcc ttgccggcgg cgccgtactt ggctaacgtc gcctcatggt tcctgtactg
     5941 cccctcctgt tcgccggaat cggcctgctg ctcatcggcc tctccgtgcc cctgatccgc
     6001 cggcgcgtga agcccaacgc cttgtatggt ttgcgtgtgc ctgccacgtt cgcggacgag
     6061 tgggtctggt atgaggccaa cgctcgcagt ggccgggacc ttctgctcct gggaaccttg
     6121 cagatcgtgg cggcctcgct cccgctgtgg attccgggtc tttccgaagc tgcctacgcg
     6181 ttgaccaatg ccggtctggt cggaatcggc gccctgctcc tgtgcatcgt cggctggctg
     6241 cgtgccaatc ggctcctgag ggagcaagag gccggccgga gcctgagcac aggccggcct
     6301 cgcgacggtg ggtagttaac cctccaggaa gcttccatcc tggagctggc ggatcgcccg
     6361 gtggagctcg gcctgggtgt tcatgacgat ggggccgtgc caggcgaccg gttcctcgag
     6421 cggctttccg gaggccagga ggaagcggat cccctcttct ccggcctgca ccaccacctc
     6481 gtcgccgcgg tcgaagacga cgagggagcg gtcgcccgcg gcttcccagg acagcgtctc
     6541 cgtgccatcg accgactcca tgagcacccc gcgcggcggc gaggcgttgg cgaagcggcc
     6601 cgagccggcg aagacgtagg cgaaggcgtt gcgggcggtc tccaccggca gcactttgcg
     6661 gcggcccggc ggcacccaga tgtcgatgta gctcggctcg gcggcgatgc cttcaaccgg
     6721 accggtcttc ccccagaagc tgccgcagag aatccgtgcc cgcgtgccgt cgtcgtcgac
     6781 cacctcgggg atctcggccg acttcacatc ctgatagcgc ggcgcggtca tcttgagcga
     6841 cgaggggagg ttcgcccaga gctggaagcc gtgcatccgt cccgactcgt ccccctgggg
     6901 catctcctga tggacgatgc cccgcccggc ggtcatccac tgcacgtctc ccgcgccgag
     6961 cgcgcccatg ttgccgagac tgtcccggtg ctccacggtt cccgagagca cgtaggtgat
     7021 ggtctcgatc ccccgatggg ggtgccacgg aaagccggcc cggtagtcgg ccggacggtc
     7081 gttgcggaaa tcgtcgagaa gcaggaaggg atcgaagtcc cccgtctccc cgaacccgaa
     7141 agcccggcgc agacgcacac cggcaccctc ggtggtggga gtggactgcg agagcgattt
     7201 gacggagcga atggacatgg cgcctccttg gtcggttgcc gttcctctga aacggggcaa
     7261 gagaggcggg aattccgggc tgtcctggct gggagagaga acccccactt ttagctgctt
     7321 gctcctgcgt atcttttggg atatacttga gacgtgctcg aagtccggca gaccgtcgag
     7381 ttcgaggagt ggtttgacgg ccttcgggat cgccaggctc gcgcacggat tgatgtgcgg
     7441 cttcggcgct tgtcgctagg caacccgggc gacgtgaagc ctgtcggtga gggggtctcg
     7501 gagctgcgga tcgattacgg tcctggttat cgagtgtatt tcattcggca aggtcaatct
     7561 gtggtcgtcc tgcttgccgg tggcgacaag cggacgcagg ctgcagatgt cgcccgggca
     7621 atcgaactgg cacatcacct gtaggggaac tctcatgatg gccaagacga agacctctcc
     7681 ttgggatcca gcgaatcatc tgaagaccga agaggatatg gccgcttatc tggaggcggc
     7741 gctggaagac ggtgaccctg cgctggtggc tgctgcgctg ggagatatcg ctcgcgccaa
     7801 gggcatgact cagatcgcac gagaggcggg gctcggacgc gagagcctgt acaagtctct
     7861 ctcacccgag ggcaaccccg agctggcgac cgtgctcaag gtagtgaagg cgatcggcct
     7921 cagattgcac gcctccacac tttagatggc ctatcaaggc ctctgcggag gggcggggct
     7981 tgcccgcccc ggagaaggtg gtcctttcgg cgttagctca ggccgaagcc atcccctcca
     8041 gctccgcccg ctcctccgga gtgaggaagg cggcgtggag ggcgttctcc tggaggcggc
     8101 gcaggtcgac ttcgtcgagg cccagctcgt actgggcgat gcggtactcg tgggcgaggt
     8161 cgatgccgct gatgctcggg tcgtcggtgt tcagggtggc gagaaggccg cggcggagga
     8221 agaaggggag ggggtgcgag cgatagtcgg ggaaggtgct cgtgtggagg ttgctggtcg
     8281 ggcagacctc cagggggatg cggcgctctt ccagcagcgc caggaccgcg gggtcttcga
     8341 tcgagcggac gccgtggccg atgcgctcgg ctcccaacgc cagcaccgct tggcggacgc
     8401 tccccgcccc ggccgcctcg ccggcgtggg cgatgacgtg cagccccgcc tcccgcgccc
     8461 gccggaaatg ctcgacgaag aggtcgcccg ggaagcccgc ctcgtccccc gccaggtcca
     8521 gggccacgac gccgcggtcg cggcagcgga tcgccgcctc cagctccagg aagccggcct
     8581 ccgggccgta gtggcgcgag atgatgacga tcagcttcgc gcggaccggg atcacccccc
     8641 gcgcctcctc cagcgcgtcg caggcggcgt cggccacggc cagggtgtcg agcccgtgct
     8701 cctcggccat gaagcggggg ctgaagcgca gctcgatgta gtcgatccgc tcgcgcgccg
     8761 cgtcctccag gttttcacgc acgatgcggc ggacggcgtc gtagtccacc atcacccggc
     8821 gcaggaccgc gaacttggcg atgaacgcca tgaggtccga ctcgcgctcc gccacctgga
     8881 cgtgcggtcg cagctcctcc agcgtccggc ccggcaggcc gatgccgtgc tgccgcgcca
     8941 ggtccagcac ggtctcgagg cggacgttgc cgtccaggtg gcggtgcagc tcgacgaggg
     9001 ggtagccgtt gcgcatccga aatgcctcct agggctcctc gttgtcctcc ggcgcctccg
     9061 acgtctccgg caccgcgtcg ctccctgcca ccatcaccac gaactccccc ttgagggccg
     9121 tccgcttgcg gagggtctcc agcagctccg acagcagacc gcgcagcacc tcctcgtgaa
     9181 gcttggtcaa ctcgcgggcg accgcggccg ggcggtcgcc cagctcggcg agggcgtcct
     9241 ccaggctcgc cagcaggcgg tggggggatt cgaagatcac cagcgtgtgg cccagccccg
     9301 cccacctctt atagaaggag cggcgcttgc cggacttcgg cgggggaaag ccggcgaagg
     9361 tgaaggggta ggggggcagg gccgaggcca ccagggccgt cagcaccgcc gaggggccgg
     9421 ggatcggctc gatccgcacc ccgcgcgccg ccgcctcgcg caccagcacg tagccggggt
     9481 ccgagaggag cggcgtgccg gcgtcgctgg cgaccgcgat caccttcccc tcctcgaggt
     9541 cggcgaggag gcgcggcagg cgctgccgct cgttgtgctc gtggagcgag accagcggct
     9601 tcttcacccc gatatgggcg aggagccggc cggtgtggcg ggtgtcctcg caggcgatca
     9661 ggtccgcctc ctcgaaggcg gcgcgggcac gcggcgacag gtcgtccaga tggccgatgg
     9721 gcgtcgcgac caccaggagc ttgccggcca tcagtcgcgc gggatttcct ggtcgaggtt
     9781 gacggcgctg ccggagaggc gggcgatcag catcagccgg cgcttgaacc ggaactgctt
     9841 ctccagccgg acgatccgct cgaccgcggc ttcgtcgtag ccccgcgcca ccacctcgtg
     9901 ggggaggagg ccgaggtcga agagcaggtg gagcacctcg tccgccgtgg cataggagaa
     9961 gcccaggtcc ccctcgtccg tctgccccgg ccagaggtcc gccgacggct tcttgtcgat
    10021 cacctggccg gggaggccga ggtgacgcga gagctgccag acctggcact tgtagaggtc
    10081 gcccagcggg ttgagcgagc tggcgttgtc gccgaagacg gtcgagtagc cgagcaggat
    10141 ctcggtcttg ttggaggtgc cgagcaccag gcagcccagc ttcttcgcct ggtcgaagag
    10201 gatggtcatg cgctcgcgcg ccattttgtt gccgcgccgg cccgggtcgg cgtccggctc
    10261 ggcggtctcg aagtaggcgt ccaccatggg cgagatgtcg atctcgcggt gcgggactcc
    10321 gaggtgcccg gcgacggcat gggcgtccgc gaccgactcg gggctggagg tgcggtacgg
    10381 cagcaggaag ccgtgcaccc gctcgggccc cagggcgcgg acggcgaggg cggcggcgag
    10441 cgaggagtcg acgcccccgg acaggccgat caccacgccg tcggtgcgcg aggtctccac
    10501 cgcgtcgcgg atgaacgagg tgaggaccgc cgacgccagc tcggcgttca gctccagccg
    10561 ggccgccacc tgctcacgcc ggatcatcgg ggccctcgat gtcgtcgcgg atgtcggcca
    10621 gcagcgggag gctccagcgc aggcggtcgg cgtcgcgcag gtcgatctcg gtgaccagca
    10681 ggtgctcctc gaggagcggc gcccgctcgg tcagctcgcc gccggggcgg acgatgtggc
    10741 tgccgccggg gtagaaggag ccctcctccc agcccacgcg gttgcagtag gcaacccagc
    10801 aggtgttgac cagtgccgtg gagcgggtga gcagctccca gttctcctgg ctggcgggga
    10861 gctggccgga cccgacccgg cccggtccgg cggagatgac cacgatcagc cgggcgccgg
    10921 cggtggcgag gcgccgggcg aggtccggat accagaggtc ctcgcagacc aggagtccgg
    10981 cgttccccga ggggaggggc gccagggcca ggcgcttgcc ggcgccgagg aagcgctcct
    11041 cctggaagat gccgtaggtc ggcaggtaga gcttgcggtg cacccagacc gggctgccgt
    11101 gctggagcag cgcggcgctg ttgtggagga tgccgcggcg gtcctcctcg acgaagccga
    11161 ggacgaccgc catcgggccg gcggcctcgg ccagacgggc gaggaccggc gaatcggccg
    11221 gcagcgctac gcgcgaggtc aggtggagga gccggtagcc ggtgagcgac agctcgggga
    11281 agaccaggag gtcgaccttc tcctcccggg cgcgctcgat ccaggccagg tggcgctcgg
    11341 cgttggcggc gacgtctccc agcgcgcagt cgatctgggc gaggccgatg cgcaggatgg
    11401 agggagcttc agccatgacc ggacgcgatg ctagcatgac ccctgaatgc gtacagttcc
    11461 cctcttccgt actttgctgg cgctcgtctg cctgaccgcc gccggtaccg ccggcgccgg
    11521 tgcccaatcc ccgaaaccgc cggcgccgac gacctcggcg cctcagggcc aggccgtgct
    11581 caaagaccgg atcatcgcgg tggtggacga ggatccgatc ctcctctcgg acctggagcg
    11641 ggtgatcgcc ctgaacctca tccgcccgcg ggagggggag gccggcgcgg cgtaccggcg
    11701 ccgggtgctg gggcagatga tcgagcagcg gctgcgcctg cacgagatcg accgcttcgg
    11761 cctggagcag gtgccggtgg acgagatcga gcgccgggtg tccgaggtcc gcgccagctt
    11821 ccccgacgag gcggcgttcc agaggacgtt gcggcagcag gggctcaccc tgccggccct
    11881 gcgccagctc ctcacccggc agctcgtggt catgacctat gtggatgagc gcctggggcc
    11941 gcgggtgttc atcgactccg aggacatccg cgcctactac gaccgcaccc tggcccccga
    12001 gatgcagcgc cgcggccagc cggtgccggc gctggaggac gtccgcgagg agatccgcac
    12061 cgtgctgcgc gagcagcggc tgaacgacga gatccgccgc tggaccaccg agctgcgcga
    12121 ggaggccgac atcgccgtct acctcgatac cccccagggg gcactccccc cggtggtcaa
    12181 gaccctcgac gggagcagcg ggaagaagag cccgcggccg tagagaccgc tagtacagcg
    12241 cctggtactc ggcccagccc gacaggacct gctggaggta gcggcgggtc tcgtccgagg
    12301 cgaccttggt gaagaactcc tcgggctcga ggctgttgca gtagctcctc cagaccacgg
    12361 cctggggctc gccggcgtcg taggcggcca ccgcgacatg ggtggcgccg ccgaaggcct
    12421 gcacgagccc ggcgaggtgg gtggccccca gcgccaccgc gacctcggga cggtagaggt
    12481 cctcgggggc gaagctgccg aggccggcct gccgcgccag gacgcgcgcc ctcgccgggg
    12541 agaggtgggt gagcccgcgc tcgccggatt cggtcaggga gagcgggtcg aaccggctct
    12601 cgttgcggat caccgcggcc agcaggtcgg ggtccacccc ccgggtgcgg ccctgggtca
    12661 tgagcagctc gcggtaggcc ggagggtgga gcgcctgccg gaaatcccgc ggctggaccc
    12721 gcagcaggac ccgctccggc gtgcggcgcc gcagctcttc ggcgagctgg atggagcgcg
    12781 gcagctcgcc ggccgaggcc aggcgccggc tgccggtgag cgccagggag gggttgcgga
    12841 ccgggaagtg cgtggcgacg gccggtcccc cttcgtgcca gagccccagc gccaggagca
    12901 tctcctcggg gcgccgcagg tcggacgacc acagcggcca ctggcccacg gggacgatcg
    12961 gcatccggag gaagggggcc gttcgggcat cggcgagcag gagctgccgg agctggaagc
    13021 cggccttgcg gcccgccggg tcgccgtccc cgaggaggag ccaggcgtcg cgggcgttgt
    13081 cgacgccccc caggttggcg cggcggcgcc cctcggccag cgtgacccgc gccagcggct
    13141 cggcggccag gcggcgatgg gccccctgcg ccagggggtg gagcgcgtcg gcgcggagga
    13201 tctccagata ggcggccacc gcggcggtgc cttcgccctg cagctcggcg aggcggccac
    13261 gccagtaggc cgactcgatc cggctgccgc ggtcccccgc ggtgcgggcg agccagccgc
    13321 cgacgcggtc ggaccgcccg cgcaccaagt ccgaggcggc gaggaagagc gaggcgcgca
    13381 cgatggtatg ccgccactcg cgccgcccgg cgagaacctg ataggtggcg agcgccggcc
    13441 cctcccggcc acgccgccag tcgaggcgca gggcggcgaa gagcgaggcc ggggcccact
    13501 ccgagctggc cggagaggcc tggtaggcgg cgccgaagct ctccacggcc ttctgccagc
    13561 gcccctgcag ctcgtgggcg cgcccctgct ggtggagggc ccgcgcccgc tgctccgggg
    13621 tccgcgcccg gaccgccagg ctgccgaagg tgccggccgc ctggtcggcg cgtccctgca
    13681 ggagctgcga ctggcccagt gcctggagca cctcgaagct gtcgcgctgc gacaggcccc
    13741 gcttgccgat ggcgcgccgc aggtattgga gggcccggtc gaggtcgtgg tgccggtaga
    13801 aggacatccc cagcagcatg ggcagccgtc cggcctcgga ttcggagacg agcccggcca
    13861 ggcggtcggc ggcggcgcgc gcggtgtcgt cccgcacgtt ctcctcgagc agcccggtca
    13921 gcaggctgcg ggcgaactcg cgcaactcgg agcgctgggc gcagtcggcc tggctgagca
    13981 gcaggagccg ccgctcccgg gccggcatgg tgtccggctt cagcggccgg aggaggcggc
    14041 agtccccccc tttttcgagg gtgcgggcga ggagctgcac ggcctcgggg aggaggggcg
    14101 aggaggggga cgcctgggcg agcgccgtgg cgatgaggcc ggcggccacc tcggggtggc
    14161 gctgccgctc ctgctcgagc gccatgcggt accggctgta gaaccggagc tccggcacgg
    14221 cgagggcgtc ggcgaaggcc tcgccggccc cccgcggatc gcccagcagc tcgaggaggt
    14281 ggccgcgcag gtagtcgagg ccgatacgcc gggaggcgtc ggggcgctcg cgggactcct
    14341 gctccaccac gtccagggca gaccgcggcc gcccctcgat ctgtagttcg acgagggccg
    14401 gacgggggtc cgtggccgcg acggccagga aaagcgtgag agtgccgagc atccgggaat
    14461 ccgggtgcgc gggcgggacg ggtcgggtct gcggcgcgga gagcgcttag gccgccgacg
    14521 ggatcgccca ccgcgccacc cgggcgccga gggcctgcaa cttcagttcc atgccttcgt
    14581 aaccccgatc caggtggtag atgcggtcga ccacggtctc cccctccgcc gccacgccgg
    14641 ccagcaccag gcaggcggag gcgcgcaggt cggtggccat gaccccggcg ccggtgagtg
    14701 gcgtcggccc gtggatcacg gccttgccgc cgtcgacgcg cacgtcggcg cccatgcggg
    14761 cgagctccgg cgcgtgctgg aagcgatttt cgaagatcgt ctcgctgatc gtggcctcgc
    14821 cggcggcctg ggtcatcagc gccatgtact gcgcctggag gtcggtgggg aagccgggat
    14881 gggggcaggt ttcgatgctt cgggcctgca gattcccgtc ggcgctcacc cggacgccct
    14941 ccggcaagac ctccactttg gcgcccacct cggccagttt gtccagcagc ggcgccagat
    15001 cggcggggac cgcctgctcc agcagcacgt cgccgccggt catcgccgcc ccgacgaggt
    15061 aggtcccgcc ctcgatgcgg tcggggatga tggcgtgctc ggagcccccc agcccgtcca
    15121 cgccctcgat ggtgatggtg gattcgccgg cgccgtggat gcgggcgccc atcgcgttga
    15181 gcaggttggc gaggtcgacc acctcgggct ccagggcgca gttctcgagc acggtcgtgc
    15241 cgcgggcgag ggcggcggcc atcaggacgt tctccgtgcc ggtgaccgtg ggcatgtcga
    15301 agcggaagcg ggcgccggtg agccgcggcg aggtggcctc cacgtagccg cggtcgaggc
    15361 ggacctcggc gcccagggcg gtgagcccct tgaggtgctg gtcgatgggg cggacgccga
    15421 tcgcgcagcc gccgggcagc gacacccggg cgtggccgag cttggcgagg agcggcccga
    15481 gcaccaggac gctcgcccgc atc
//
LOCUS       DASPJI010000227         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_644265, whole genome shotgun sequence.
ACCESSION   DASPJI010000227 DASPJI010000000
VERSION     DASPJI010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644265"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VEL74_06010"
     CDS             <1..497
                     /locus_tag="VEL74_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG62115.1"
                     /translation="PRPAMDGRYIETTRQDWLTALEISAYSLVSVAREAEPLLSEGGG
                     IVTLTYYAAEKVVPKYNVMGIAKSALEASVRYLAYELGKKGIRVNAVSAGPVRTVAAR
                     SIPGFMKMFNRVADMAPLGRNVTHEEVGNLGLFLLSPLALGITGETVYVDAGFHVMGM
                     ELGE"
     gene            complement(575..991)
                     /locus_tag="VEL74_06015"
     CDS             complement(575..991)
                     /locus_tag="VEL74_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62116.1"
                     /translation="MPILPYKQDGIRKEMENLLKKLEVNIDELPQFTVPRVKLQNLLT
                     EIVTLSNEQNTYRYSKQQTTKRLTAALEEGRKLLNFFWVGLKEHYGTRNEKLVEFGLQ
                     PYRGRARKEEEPTVKKPAAQPAAPETGKPEVDEPES"
     gene            complement(1046..1408)
                     /locus_tag="VEL74_06020"
     CDS             complement(1046..1408)
                     /locus_tag="VEL74_06020"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HYG62117.1"
                     /translation="METLQNKTNEPVQRKLIRPRGEKLKAERVQDKLKAERVQDKLRS
                     MPGWRFARRGQTLHRVREFPDHQVASAFAGFAGAFAARAKQPADITLSGSRVLVVLHP
                     KSRGGLSDAVLDLAKSLG"
     gene            complement(1718..>2254)
                     /locus_tag="VEL74_06025"
     CDS             complement(1718..>2254)
                     /locus_tag="VEL74_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62118.1"
                     /translation="LSMELLDPHTHPGLAARACLALALGLVDIEMAADARAALRGAHR
                     IYGQIRAELEQVRVYWLEGRVLARLGETEQAFALMEQILAKLFAEGLMAEAAVAGVEL
                     AGLLASLGRAGEIAERLAEVRRACAADLRLQGATETLAWAASAAGSELAERASCSACA
                     LRIALRLAGKRVEPPPAA"
BASE COUNT          350 a          749 c          756 g          399 t
ORIGIN      
        1 ccccgcggcc ggccatggac ggccgctaca tcgagaccac gcgccaggac tggctgacgg
       61 cgctggagat ctcggcctac tcgctggtct cggtggcgcg ggaggccgag ccgctgctca
      121 gcgagggggg cgggatcgtc accctgacct actacgccgc cgagaaggtg gtgccgaagt
      181 acaacgtcat gggcatcgcc aagagcgcgt tggaggcgag cgtgcggtat ctcgcgtacg
      241 agctgggcaa gaaggggata cgggtcaacg ccgtctccgc cggcccggtg cgcaccgtcg
      301 cagcgcggag catccctggc ttcatgaaga tgttcaaccg ggtggccgac atggcccccc
      361 tgggccgcaa cgtcacccac gaagaggtgg gcaacctcgg cctcttcctc ctctcccccc
      421 tcgccctggg catcaccggc gagaccgtct acgtggacgc cggcttccac gtgatgggga
      481 tggagctggg ggagtagcgc gggcctcgtc aatgggataa aaaggccggg gcgtgcacgc
      541 acacgccccg gcggttcagc ggatcaacgg cacatcagga ctcgggctcg tccacctcgg
      601 gcttcccggt ctcaggagcc gccggctggg ccgccggctt cttgaccgtc ggctcctcct
      661 ccttgcgcgc ccggccgcga tacggctgca ggccgaactc caccagcttc tcgttgcgcg
      721 tcccgtagtg ctccttgagg ccgacccaga agaagttcaa cagcttgcgg ccttcctcca
      781 gcgcagccgt gaggcgcttg gtcgtctgct gcttgctgta gcggtaggta ttctgctcgt
      841 tcgagagcgt cacgatctcg gtgagcaggt tctgcagctt gacgcgcggc accgtgaact
      901 ggggcagctc gtcaatgttg acctccagct tcttcaggag gttctccatc tccttgcgga
      961 taccgtcctg tttgtagggc agaatgggca tcttcaattc ctttcgttcc atgatccttc
     1021 gtcggtttga ggggtgcggg ccttttcagc ccagggactt ggcgagatcg aggaccgcgt
     1081 cggacagccc gccgcggctc ttcggatgga ggaccaccag cacccgtgat cccgagaggg
     1141 tgatgtccgc gggctgcttc gcacgggcgg cgaacgctcc ggcgaagccg gcgaaggccg
     1201 aggccacctg gtggtccggg aactcgcgca cccggtggag ggtctgcccg cgccgggcga
     1261 accgccaccc cggcatcgag cgcagcttgt cctgcacccg ctcggccttc agcttgtcct
     1321 ggacgcgctc ggccttgagc ttctcccccc gcgggcggat cagcttcctc tgaacgggct
     1381 cgttggtctt gttctgcagc gtttccatac agctactcct ttgtaagttc cgggccttca
     1441 tggcaccgga aagtcgatct cctggtcatg ccggcgggct gatcggcgat tcgccttcct
     1501 gccctcctgt ctatgtatac ggatgaaccc gttcgcgcgt cgaccgtttt gggaaaattt
     1561 tttcgacaaa atttataggg attcggttta agcagagcaa tagctgaccg gcaggctgga
     1621 gtgccgaaaa aggacgaggc gatggttggg gacccgcggg ttccggttag acggcggccg
     1681 gcacggctcg gcgggggtgc tgcggtgcta cagactttca ggccgccgga gggggttcga
     1741 cccgcttccc ggccaagcgc agtgcgattc gtagggcaca agccgagcaa gaagcccgtt
     1801 ctgccagctc cgagccggcg gccgaagccg cccaggccag cgtctcggtc gctccctgga
     1861 ggcgcaggtc ggcggcgcag gcccggcgca cttcggccag gcgctcggcg atctcgccgg
     1921 cgcggccgag ggaggcgagc agtccggcga gctcgacgcc ggccacggcc gcctcggcca
     1981 tcaaaccttc ggcgaagagc ttggccagga tctgctccat caaggcgaag gcctgctcgg
     2041 tttccccgag gcgcgccagc acccggcctt ccagccagta gacgcggacc tgttccagct
     2101 cggcgcggat ctggccatag atcctgtgag cgccccggag agcagcgcgg gcgtcggcgg
     2161 ccatctcgat gtccaccagc cccagagcca gcgcaaggca tgcccgcgcc gccagccccg
     2221 gatgcgtgtg aggatccagc agctccatgg agag
//
LOCUS       DASPJI010000228         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_188034, whole genome shotgun sequence.
ACCESSION   DASPJI010000228 DASPJI010000000
VERSION     DASPJI010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188034"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..794
                     /locus_tag="VEL74_06030"
     CDS             144..794
                     /locus_tag="VEL74_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HYG62119.1"
                     /translation="MAGRWGSRRFDLEQADRRLLQVVTDAMDGAAALSGHRLACRPGC
                     DECCHGPFPINALDAWRLRRGLAELAARDPERAAAVVDRARTLVPLLAADFPGAPELG
                     VLSEDEEAEEAYFGRHAALPCPALDPVTRRCDLYAYRPLTCRTFGPPVRLGAADLPPC
                     RLCFQGEPEAAIESCRAEPDPHGLEDRLVARLGQDEETLIAFVLADRLHKGEDESS"
     gene            831..1328
                     /locus_tag="VEL74_06035"
     CDS             831..1328
                     /locus_tag="VEL74_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62120.1"
                     /translation="MSEKTTGRVVRGLAIAMFFLALVPTQGRAVDFGVRGGLYSDAEA
                     GFVGVELLTPVTRKWFFNPNLEYVFVDDGDLYTGNLDFHYDFDTRNPFYLWAGAGAAI
                     IRSEADPPAFCRNCDSDAETDLGVNLLGGIGFGKGQALRPYVQGKVILSDETEASIAV
                     GIRFH"
     gene            complement(1322..1798)
                     /locus_tag="VEL74_06040"
     CDS             complement(1322..1798)
                     /locus_tag="VEL74_06040"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYG62121.1"
                     /translation="MTMRTTRRAMLALVLLVLTGDGAYAQAPNAEADVRAADAQRFAA
                     MVARDRQALERLLADDLTYTHSTGQVESKAQFLESIAAGGLVYQAIDPEEVAVRVYGE
                     TAVVTGRASLRVESRGQALVLPVRFTSVYVRKDGLWRLAAWQSTRLPEPSKPPSPQ"
     gene            1837..>2359
                     /locus_tag="VEL74_06045"
     CDS             1837..>2359
                     /locus_tag="VEL74_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62122.1"
                     /translation="MPAIRVHLLILGLALLSAVPSGQAQTPPAASAPQAAPATPAAPE
                     PTMSFEEYDPRSTLVVPEHPVPRARYPFIDVHSHQRASTMTSEQVDELVAAMDGLNMA
                     VMVNLSGGSGETFQKGWTHLSSRHAKRFIQFANVDFEKISEPGFGERAAAQLAEDVKN
                     GARGLKVFKNLGMF"
BASE COUNT          342 a          850 c          798 g          369 t
ORIGIN      
        1 cctccatcac cgtgctccag ggccgggcct ccgggggccg cagggggcgc acggcgtggc
       61 ggagccgcgg agccatcgtc cagacgtggg gcagcagggg catggcggaa tggtaaccgc
      121 aagggggtgt tatggtgggg atgatggctg gacgatgggg ctctcgccgt ttcgacctcg
      181 agcaggccga ccgccggctc ctgcaggtag tcaccgacgc catggacggc gccgcggccc
      241 tctccggcca tcgcctcgcc tgccggccgg gctgcgacga gtgctgccac gggccgttcc
      301 ccatcaacgc cctggacgcc tggcgcctcc gccgggggct ggccgagctg gctgcccggg
      361 atcccgagcg cgccgcggcc gtggtggatc gcgcgcggac gctggtccct cttctcgccg
      421 cggatttccc cggcgccccg gagctcgggg tcctgtccga ggacgaggag gcggaggagg
      481 cctacttcgg ccgccacgca gcgctcccct gccccgccct cgatcccgtg acccggcgct
      541 gcgacctcta cgcctaccgg cccctgacct gccgcacctt cggcccgccg gtccgcctcg
      601 gcgccgcgga cctccccccg tgccgcctct gcttccaggg cgagccggaa gcggccattg
      661 aatcctgccg cgccgaaccg gacccgcacg gcctcgaaga ccgcttggta gcccgcctcg
      721 gccaggatga ggagaccctc atcgccttcg tcctggcaga ccgcttgcac aagggcgagg
      781 atgagagctc atagagagtt ctgcattctt tgtgtcagca ggaggttccg atgagcgaaa
      841 agacgactgg acgcgtggtg cgcggactcg ccatcgccat gttcttcctc gccctggtgc
      901 cgacgcaggg ccgggccgtg gatttcggag tccggggcgg cctctacagc gatgccgagg
      961 cgggcttcgt cggcgtggag ctgctcacgc cggtgacccg gaagtggttc ttcaacccca
     1021 acctcgagta tgtcttcgtg gacgatggcg acctctacac gggcaacctc gacttccact
     1081 acgacttcga cacgcgcaat ccgttctacc tttgggccgg cgcgggcgcg gcgatcatcc
     1141 gttccgaggc cgatccaccg gccttctgcc ggaactgtga ttcggacgcc gagaccgacc
     1201 tgggtgtgaa cctcctgggc ggcatcggct tcggcaaggg gcaggcgctc cggccgtacg
     1261 tccagggcaa ggtgatcctg tcggacgaga ccgaggcgtc gatcgccgtc ggcatccgct
     1321 ttcattgagg tgacggaggc ttggagggct cgggcagccg cgtggactgc caggcggcca
     1381 gccgccagag cccatccttc ctgacgtaga cggaggtgaa ccggaccggg aggaccagag
     1441 cctgcccccg gctctcgacc cgcagggacg cccggccggt caccaccgcc gtctcgccgt
     1501 agacccgcac cgccacctcc tccggatcga tggcctggta gaccaggcca ccggctgcga
     1561 tcgactccag gaactgggcc ttgctctcca cctgcccggt ggagtgggtg taggtaaggt
     1621 cgtccgccag gaggcgctcc agcgcctggc ggtcccgggc gaccatggcc gcgaaccgct
     1681 gggcatcggc ggcccggacg tcggcctcgg cattcggagc ctgggcgtag gccccgtcac
     1741 cggtcagaac caggagaacc agcgcgagca tcgcccgccg ggtcgtacgc atcgtcatcc
     1801 tctctccttt cgaagctcct ccagtatatt gacgcgatgc cagcgatccg tgtccatctc
     1861 ctgatcctcg gcctcgccct gctgagcgcc gtcccttccg gccaggccca gacgcctccg
     1921 gccgcctccg ctccccaggc tgccccggca actccggcgg ctccggagcc caccatgtcc
     1981 ttcgaggagt acgacccgcg ctcgacgctg gtggtgccgg agcatccggt gccgcgagcc
     2041 cgctacccct tcatcgacgt ccacagccac cagcgggcgt cgaccatgac gtcggagcag
     2101 gtggacgagt tggtggcggc catggacggg ctcaacatgg cggtgatggt gaacctctcg
     2161 ggcggcagcg gcgagacctt ccagaagggc tggacccatc tctccagccg gcacgccaag
     2221 cggttcatcc agttcgccaa cgtcgacttc gagaagatct cggagcccgg cttcggcgag
     2281 cgggcggcgg cgcagctcgc ggaggacgtg aagaacggcg cccgcggcct caaggtgttc
     2341 aagaacctgg ggatgttcc
//
LOCUS       DASPJI010000229         5589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_527685, whole genome shotgun sequence.
ACCESSION   DASPJI010000229 DASPJI010000000
VERSION     DASPJI010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527685"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VEL74_06050"
     CDS             complement(<1..259)
                     /locus_tag="VEL74_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62123.1"
                     /translation="MDDAFPPIDSPILSEATSQALEFPGLLALVAQLAASDLGRERAL
                     SLRPFAEEEGLRTHRRRFEEVSRLLADGPVAPSFDVPVGALL"
     gene            complement(359..1804)
                     /locus_tag="VEL74_06055"
     CDS             complement(359..1804)
                     /locus_tag="VEL74_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62124.1"
                     /translation="MPELHPDTDLLERFVRGELEIAESRGIVRHLVTGCPLCIAETRR
                     LWKRAPSPEACDIAYDTVLDRLLERHGPALERLAAERRAAPGLLGELLRQPPSRRVAL
                     VHADGRFRSLPLCELLLDRVRDADPGQPAQAIGALEAAELALAVAEKLDGKLDGTATL
                     VRGVQARAWAWLGNARRLAADLPGARRALDTAELLLGQAAAGAAGAAGTVGPADPLEE
                     AEVLALAAQLHAEQRQPRHLDEAAARFDRAAALYRAAGERHLRGRLLIQKGMALGEEA
                     LRQSSLEQAVFGEPGWGLTAVAALQEGLALAGEGLERPLRARALAGLVTMLLEAGRPE
                     ETGGPLRELRRLHEEDGDALALTRLRWLEGKADEALGRFDDAEAALMEARAGFLRQRM
                     GPEAAFVSLELAVVYARRGRDPEMRRLSDDMSPVYLAKGVTRDVVSALIVFQRTAEFE
                     PGNLTFFIEVARFLGGSARLRRSMRPLQWVS"
     gene            2045..2563
                     /locus_tag="VEL74_06060"
     CDS             2045..2563
                     /locus_tag="VEL74_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG62125.1"
                     /translation="MSIFHGLGRALRWLRDRQGKRQYQVADAAGITKAMLSAYETGKQ
                     KPSLETLEKILTALDCDLNDLHNALQIVNERPEAIRRPGALQQGGGMWPPFRTGLPEP
                     EGFDSGPDVYRVLGLSRALPPSAEQALSEMLDGFHKFLRYLHGRTVGSSAPVPPPPPP
                     VSDDSEPFDEAP"
     gene            2737..4734
                     /locus_tag="VEL74_06065"
     CDS             2737..4734
                     /locus_tag="VEL74_06065"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HYG62126.1"
                     /translation="MEPRGAASYRGGSGEPAWSNGSASRRVHGDLERRVARLRHGDHL
                     CLIYDAVQEQTAALVAFVKGGLAAGDRCLFVGHPVSGSRLERALAEAGVRAGREAERG
                     ALVLLSDREMWLPAGRFEPGAVMDLLRQAEQQALDDGFSGLRASWDLGWVLEGIPGSE
                     QLAPPLTDRLVELEAHLNRFLSGSRTAALCRYPRRHSPPELVQDILRTHPLALLGDQL
                     CPNVFYEPSEMVLDHPTSGERVDWMIARLRSARLNEQKLEEVNFRLSQSRAALARADR
                     AKEDLLGMLAHELRNPLGTISNALQILRLQGEGDETWKRTLDAAERQVLHQALLVDEL
                     LEASRVTRGEVELNCEQLDLGRLVRETVEGYRETLRNSGLHLELELPEGPLPVHGDRL
                     RLSQVLANLLRNAAKFTSPGGRVWVRAGRAAEGGRAEVTVRDDGVGIPPEVLPHVFEI
                     FTQADRSLDRSAGGLGVGLAVVRGLVDMHGGEVEARSAGAGQGAELTLRLPLAAGAQV
                     SRPAEAGLPAEPAGPLSAHEEARRILVVEDNPDAAATMRDFLELSGHQVALANNGTEG
                     VQAARDFHPEVVLCDLGLPGMDGFQVAAALRSDPATASAKLIAVTGYGRDEDRRRSRE
                     AGFDVHLTKPVDPAQLKRVIQEEVPVATAPPEPVRQAGSSA"
     gene            complement(4713..>5589)
                     /locus_tag="VEL74_06070"
     CDS             complement(4713..>5589)
                     /locus_tag="VEL74_06070"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HYG62127.1"
                     /translation="GLQHGGIVRADPNTLVAMGDLTTGPALLTLFGLALSLALMAAGV
                     ATAIFWGLVATLAAALATGVLAPPAALLSAPSLALPGLQIDLLGALRLEYLPSLLVLL
                     FFALFDTLGTLMGVAHEAGFLRDGKLPRIERALAADAGGMVAGALFGTPPVTSYIESG
                     AGVGVGARTGLASLATGVLLLASLFLAPLVGWMGQATAEGLTPVTAPALIVVGTLMVR
                     AVREIDWNDATEAAPAFFTALLMPLTFNISHGLAAGIVVYVLVKLAARRWREVHWLMA
                     VLAALFVLRYALLPA"
BASE COUNT          814 a         1983 c         2093 g          699 t
ORIGIN      
        1 ccagcagggc gccgaccggc acgtcgaacg agggggcgac ggggccgtcc gccagcaggc
       61 gcgacacctc ctcgaagcgg cggcggtggg tgcgcagccc ctcctcctcg gcgaacgggc
      121 ggagggagag ggcgcgctcc cggccgaggt cgctggccgc gagctgcgcc acgagggcga
      181 gcaggcccgg gaactccagc gcctgggagg tcgcctccga gaggatgggc gaatcgatag
      241 gaggaaatgc gtcgtccacg aggtccagtc tatctccgat gctcgtctcc gtgccgcccc
      301 ctatctaata aggaagcggc gccttgtccg ccgggccccg aatgaagaga agagggcctc
      361 agctcaccca ctgcaggggg cgcatggagc ggcggagccg ggcggagccg ccgaggaacc
      421 tggcgacctc gatgaaaaac gtcaggttgc cgggctcgaa ttccgcggtg cgctggaaga
      481 cgatcagggc cgagaccacg tcgcgggtca cgcccttggc gaggtagacg ggggacatgt
      541 cgtccgacag ccggcgcatc tcggggtcgc ggccgcggcg ggcatagacc acggccagct
      601 cgagggagac gaaggcggcc tccggcccca tccgctggcg caggaagccg gcccgcgcct
      661 ccatcagcgc cgcctcggcg tcgtcgaagc ggccgagcgc ctcgtcggcc ttgccctcca
      721 gccagcgcag gcgggtgagg gcgagggcgt cgccgtcctc ctcgtggagc cggcgcagct
      781 cgcgcaacgg cccgccggtc tcctccggcc ggccggcttc gagcagcatg gtgaccagcc
      841 cggcgagggc gcgggcgcgc agcggccgct cgaggccctc cccggcgagc gcgagcccct
      901 cctggagcgc cgcgaccgcg gtgagccccc agccgggctc gccgaagacg gcctgctcga
      961 gggaggactg gcgtagcgcc tcctctccca gggccatccc cttctggatc agcagccggc
     1021 cgcggaggtg gcgctcgccc gcggcccggt agagggcggc ggcgcggtcg aagcgggccg
     1081 ccgcctcgtc gagatggcgg ggctgccgct gctcggcatg gagctgggcg gcgagggcga
     1141 gcacctcggc ctcctccagc ggatcggccg gcccgaccgt gccggccgct ccggccgctc
     1201 cggcggcggc ctggccgagg aggagctcgg cggtgtcgag ggcgcgccgg gcgcctggga
     1261 ggtcggcggc gagccggcgc gcgttgccca gccaggccca ggcgcgggcc tgaacgccgc
     1321 ggacgagcgt ggcggtgccg tccagcttcc cgtccagctt ctcggcgacg gccagggcga
     1381 gctcggcggc ctcgagcgcg ccgatggcct gggcgggctg gccgggatcg gcgtcgcgga
     1441 cccggtcgag cagcagctcg cagaggggga gggagcggaa gcggccgtcg gcgtgcacca
     1501 gggcgacccg gcgggagggg ggctggcgca gcagctcgcc gagcagccca ggggccgcgc
     1561 ggcgctccgc cgccagccgc tccagggcgg ggccgtggcg ctccagcagg cggtcgagga
     1621 cggtatcgta ggcgatgtcg caggcttccg gggacggcgc ccgcttccag agccggcgcg
     1681 tctcggcgat gcagaggggg cagccggtga ccagatggcg gacgatcccg cggctctccg
     1741 cgatttccag ctcgccgcgg acgaaccgtt ccaggaggtc cgtgtcaggg tgtaactcgg
     1801 gcatgatctc cccctttttt gcgatccgga agcaccgaag acccggcgaa accagtttgc
     1861 tgaaaacaaa attcgaagtg gctatcgaac acatcttgtc gcggaaaaca actgtagcgg
     1921 acatatcgcc ccggcgtcaa gcaacggggg agctggaaaa gagaatctcg tgttcgatct
     1981 aagtcattga tctagaaaag cttgcgcttg caacgagggc gtgggagagg atacatttcc
     2041 gcgcatgtcc atctttcatg gccttggaag ggcccttcgc tggctccgtg acaggcaggg
     2101 caagagacag taccaggtgg cggacgcggc cggaatcaca aaggccatgc tttccgctta
     2161 cgagaccggc aagcagaagc cgtcgctcga gaccctggaa aagattctca ctgccctgga
     2221 ctgtgatctc aatgacctcc acaacgctct gcagatcgtg aacgagcgtc cggaagcgat
     2281 ccgtcgcccc ggtgctctgc agcagggtgg cggcatgtgg ccgccgttcc gcaccggtct
     2341 gcccgaaccc gaaggcttcg actcgggtcc ggacgtctac cgcgtcctgg gcctcagccg
     2401 ggcactgcct ccgagtgcgg aacaggcgct gagcgagatg ctggacggct tccacaagtt
     2461 cctgcgctat ctccatggca ggacggtcgg aagctccgct ccggtgcctc cgcctccgcc
     2521 gccggtttcg gacgacagcg agccgttcga cgaggctccc tgactcccgg gctccggagc
     2581 ttttctccgg tcccggctcc cctcgcaggc gtcctgccgt agaggggagc gtgccctgcg
     2641 cacagatctt gcactgtgcc ctccgcgtgc cgtgagatcc actcacggcc cgcggacgca
     2701 cggacgcgtc gcgagtatac aggcgaggcg gagatcatgg agccgagggg tgctgcgagc
     2761 tatcggggtg gttcaggcga gcccgcctgg tccaacggaa gcgcgagccg tcgcgtccac
     2821 ggcgacctcg aacgccgcgt ggcccgcctg cgccacggcg accacctctg tctgatctac
     2881 gacgcggtcc aggagcagac ggcggcgctg gtcgccttcg tcaagggggg cctcgccgcg
     2941 ggcgaccgct gcctgttcgt cggccatccg gtgagcggca gccgcctgga gcgggccctc
     3001 gccgaggccg gggtccgggc cggccgcgag gccgagcgcg gcgccctcgt cctgctgtcc
     3061 gaccgcgaga tgtggctgcc ggccggccgc ttcgagccgg gcgcggtcat ggacctgctg
     3121 cgccaggcgg agcagcaggc gctggacgac ggcttctccg gcctgcgcgc gagctgggac
     3181 ctgggctggg tcctcgaagg gatccccggc tccgagcaac tggcgccgcc gctgaccgac
     3241 cgcctggtgg agctcgaggc ccacctcaac cgcttcctct ccggcagccg caccgcggcc
     3301 ctctgccgtt acccccggcg ccattcgccc cccgagctgg tgcaggacat cctgcgcacc
     3361 catccgctgg cgctgctcgg cgaccagctc tgcccgaacg tcttctacga gccgtcggag
     3421 atggtcctcg accacccgac gtccggcgag cgggtggact ggatgatcgc ccggctgcgc
     3481 agcgcccggc tcaacgagca gaagctcgag gaggtcaatt tccggctgtc gcagagccgg
     3541 gcggcgctgg cgcgggcgga ccgggccaag gaggacctgc tgggcatgct cgcccacgag
     3601 ctgcgcaacc cgctggggac gatcagcaac gcgctgcaga tcctgcgcct gcagggggag
     3661 ggggacgaga cctggaagcg gaccctcgac gccgccgaac gccaggtgct ccaccaggcg
     3721 ctgctggtcg acgagctgct cgaggcgtcg cgggtgaccc gcggcgaagt cgagctgaac
     3781 tgcgagcagc tcgacctcgg ccgcctggtg cgcgagacgg tggagggcta ccgggagacg
     3841 ctgcgcaact cgggcctgca tctcgagctg gagctgcccg aggggcccct gcccgtgcac
     3901 ggcgaccggc tgcgcctctc ccaggtgctg gccaacctgc tgcgcaacgc ggccaagttc
     3961 acctctccgg gcggccgcgt ctgggtgcgg gcggggcgcg ccgccgaggg ggggcgcgcc
     4021 gaggtgacgg tgcgcgacga cggtgtgggc atcccgccgg aggtgctccc gcacgtgttc
     4081 gagatcttca cccaggccga ccgcagcctc gaccggtccg ccggcgggct gggggtgggg
     4141 ctcgccgtcg tccgcggcct cgtcgacatg cacggcggcg aagtggaggc gcggagcgcc
     4201 ggcgccggcc agggggccga gctcaccctg cgcctgccgc tcgcggccgg cgcccaggtc
     4261 tcccggcccg ccgaggccgg tttgcccgcc gagcccgccg ggccgctctc cgcgcacgag
     4321 gaggcgcggc gcatcctggt ggtggaggac aaccccgacg cggcggcgac catgcgcgac
     4381 ttcctggagc tctccggcca ccaggtggcg ctcgccaaca acggcaccga gggggtccag
     4441 gcggcccgcg acttccatcc ggaggtggtg ctctgcgacc tggggctgcc cggcatggac
     4501 ggcttccagg tggcggcggc gctgcgcagc gatccggcga ccgcctcggc gaagctgatc
     4561 gcggtcaccg gctacggccg cgacgaggac cgccgccgct cgcgggaggc gggcttcgac
     4621 gtccacctca ccaagccggt ggaccccgcg cagctcaagc gggtgatcca ggaggaggtg
     4681 cccgtcgcca ccgctccgcc ggagcccgtc cgtcaggccg gcagcagcgc gtagcgcagg
     4741 acgaagaggg cggcgagcac cgccatcagc cagtgcacct cgcgccatcg ccgggcggcc
     4801 agcttcacca gcacatagac gacgatgccg gccgccaggc cgtgggagat gttgaaggtg
     4861 agcggcatca ggagcgcggt gaagaaggcg ggggccgcct ccgtggcgtc gttccagtcg
     4921 atctcgcgca ccgcccgcac catcagcgtc cccaccacga tcagcgccgg cgccgtcacc
     4981 ggcgtcagcc cctccgccgt cgcctgcccc atccatccca ccagcggcgc caggaagagg
     5041 ctggcgagga gcagcacgcc cgtggcgagg ctggcgaggc cggtccgggc gcccacgccc
     5101 accccggcgc cgctctcgat gtaggaggtc accggcggag tgccgaagag cgcgccggcc
     5161 accatgccgc cggcgtccgc cgccagcgcc cgctcgatgc gcggcagctt cccgtcgcgc
     5221 aggaagccgg cctcgtgggc gacgcccatc agcgtcccga gggtgtcgaa gagggcgaag
     5281 aagagcagca ccagcaggga ggggaggtac tcgagccgca gcgcgccgag caggtcgatc
     5341 tgcaggccgg gcagcgccag gctgggcgcc gacagcagcg cggccggcgg cgccaggacg
     5401 ccggtggcga gggcggccgc cagggtcgcg accagacccc agaagatggc ggtggcgacg
     5461 ccggccgcca tcagcgcgag gctgagggcg aggccgaaca gggtgaggag ggccgggccg
     5521 gtcgtcaggt cccccatggc gaccagggtg ttggggtccg cccgcacgat gccgccgtgc
     5581 tgcaggccg
//
LOCUS       DASPJI010000230         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_699682, whole genome shotgun sequence.
ACCESSION   DASPJI010000230 DASPJI010000000
VERSION     DASPJI010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699682"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..>2189
                     /locus_tag="VEL74_06075"
     CDS             60..>2189
                     /locus_tag="VEL74_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG62128.1"
                     /translation="MRWTEALEELGQDVRYAGRQLWKSPAFTLVAVLTLALGTGVTTA
                     IYSVVDTVLLRPLPFAEPDRLVRVLPLTREGSQDAFSVLNFLDWRRQSTSTGGSRGAA
                     SYDGVTLNLSGDGEPERLQGMSVSASFFSVLGVKPLRGRFFTEGDERAGAPRVVVVSE
                     ELWRRSFGADPGLVGRAITLNGEPSTVVGITARGARYPFKPDLWIPFVLDEDALNPTN
                     RGALFLNAIARLEPGVSLEKARAEAAAIARRLELQYPEANTGMGMTVEPMQSDIVADV
                     RLPLLVLMGAVVCVLLIACVNVANLLLVRAAARESEIAIRSALGAGRSRIIRQLLTES
                     VGLALLGGAAGVLLAHWLTRLLVRLAPEGTPRLDEAGIDAPVLLFGLGVTILTGLLFG
                     LVPALQASRPDLNRTLKEGARGAKGGGGRVRHALVVVETALAVILLAGAGLLIQSFAR
                     LQQVDLGFDTEGVVTFRFVLPGTKYPERPQIESFVNGIVERVERLPGVRTAGAGFIMP
                     LSGSDFSLSFTVEGRPPVPQGQEPAIKVQVATPEFFQALGIPLVRGRIFDARDRQGAP
                     RVVLLNQAAARRYFPGENPLGKRIQLGWVRDDERQGGEVVGIVGDMKQVGLDQPAEPE
                     LYMPFAQSPVASISVVMRASGDPAALAAAARAQVREADPDLPIYNLGPLSDSIAAASA
                     QPRFYMLLLGAFAAVALLLAAVGIYGVM"
BASE COUNT          274 a          774 c          800 g          341 t
ORIGIN      
        1 gggatctgca gggaacccgc gagtactgcc gcgctctcgg cgagcgcaag gagaagcgca
       61 tgcgatggac ggaagcgctc gaggagctcg gccaggacgt ccggtacgcc ggccggcagc
      121 tctggaagag cccggcgttc accctggtgg cggtgctgac cctcgccttg ggcacggggg
      181 tcaccacggc gatctacagc gtcgtggaca ccgtgctgct ccggcccctc cccttcgccg
      241 agccggaccg gctggtgcgc gtcctcccct tgacccgtga gggcagccaa gacgccttct
      301 ccgtcctcaa cttcctggat tggcggcggc agagcacctc gaccggcggc agccgcggtg
      361 ccgcgtccta tgacggcgtg accctcaacc tgagcggcga cggcgagccc gagcggctgc
      421 aggggatgtc ggtgagcgcc agcttcttct cggtcctggg tgtgaagccg ctccgcggca
      481 ggttcttcac cgagggagac gagcgcgccg gcgcaccccg ggtggtggtg gtctcggaag
      541 agctctggcg gcgcagcttc ggcgccgacc ccggcctcgt cggccgggcg atcacgttga
      601 acggcgagcc ctccacggtc gtggggatca cggcgcgggg ggcccgctat ccgttcaagc
      661 cggacctctg gatccccttc gtgctggacg aagacgcgct gaatcccacc aaccgcggcg
      721 ccctcttcct caacgccatc gcccgcctcg agccgggggt gtcgctcgag aaggcgcggg
      781 cggaggcggc ggccatcgcc cggcggctcg agctccaata ccccgaggcc aacacgggca
      841 tgggcatgac ggtggagccg atgcagtccg acatcgtggc ggacgtgcgc ctcccgctcc
      901 tcgtcctcat gggggccgtg gtgtgcgtcc tgctcatcgc ctgcgtcaac gtggcgaacc
      961 tgctgctggt gcgggcggcg gcgcgggaga gcgagatcgc catccgctcg gccctgggag
     1021 cgggccggtc gcgcatcatc cggcagctcc tgacggagag cgtcggcctc gccctgctcg
     1081 gaggtgcggc cggcgtcctc ctggcgcact ggctgacccg gctgctggtc cgtctcgcgc
     1141 cggaggggac gccgcggctc gacgaggcgg gcatcgacgc gccggtgctg ctcttcggcc
     1201 tcggggtgac gatcctcacc ggcctcctct tcgggctcgt gccggcgctc caggcttcgc
     1261 ggccggacct gaaccgcacg ctcaaggagg gggcgcgcgg cgccaagggg ggcggcggcc
     1321 gggtgcgcca cgccctggtg gtggtagaga ccgctctggc ggtcatcctc cttgccggcg
     1381 ccggcctcct catccagagc ttcgcccggc tgcagcaggt cgatctcggc ttcgacaccg
     1441 agggtgtggt gacgttccgc ttcgtgctcc ccgggaccaa gtatccggag cgcccgcaga
     1501 tcgagagctt cgtcaacggt atcgtggagc gggtggagcg gctcccgggc gtccgcaccg
     1561 ccggagccgg cttcatcatg ccgctgtccg gctcggactt cagcctttcg ttcaccgtgg
     1621 aggggaggcc tcccgtgccc cagggtcagg agcctgccat caaggtccag gtagccactc
     1681 cggagttctt ccaggctctg ggcatcccgc tggtgcgcgg ccgcatcttc gacgcgcggg
     1741 accgccaggg agcgccgcgc gtggtgctgc tgaaccaggc ggcggcgcgc cgctacttcc
     1801 cgggagagaa ccctctgggc aagcgtatcc agctcggctg ggtgcgggac gacgagcgcc
     1861 agggcggcga ggtggtgggg atcgtcggcg acatgaaaca ggtgggcctc gaccagccgg
     1921 cggagcctga gctctacatg ccgttcgccc agtcgccggt tgcgtccatc tcggtggtga
     1981 tgcgcgcctc cggcgatccg gcggcgctgg ccgcggcggc gcgggcccag gtccgggagg
     2041 cggaccccga cctgcccatc tacaacctcg gtccgctgag cgactccatc gccgccgcct
     2101 ccgcccagcc acggttctac atgctcctgc tgggcgcctt cgcggcggtg gcgctgctcc
     2161 tcgcggcggt ggggatctac ggcgtgatg
//
LOCUS       DASPJI010000231        11743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_13665, whole genome shotgun sequence.
ACCESSION   DASPJI010000231 DASPJI010000000
VERSION     DASPJI010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11743)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11743
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13665"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VEL74_06080"
     CDS             <1..937
                     /locus_tag="VEL74_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012410.4"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HYG62129.1"
                     /translation="VDSPDLALNVSREMLQQDRHITLIRKGLTKKVLDVLDEMRDKEP
                     DTYLNFWKEFGRAIKEGVSSDYENKEKLLELLVFQSSNHPEKLTTLREYVSRMKEDQK
                     EIFYLTGDSRAQVENSPHLEALKDKGIEVLYLIDTVDELLTQSLHEYDGKKLKSVGKG
                     TVNLGNEEEKKQTEEALKAKEEELKPLLDAMQQKLDTWVKQVRLTNRLTTSPVCLVGA
                     EHDYSPQLEKLLQKGKGGGPKQRRIMELNPNHQILGRLKERFEQNAEDPLVEDYAQLL
                     FGYGLISEGAELEDPVRFNRAMADLMTQNLGTPAA"
     gene            complement(984..1754)
                     /locus_tag="VEL74_06085"
     CDS             complement(984..1754)
                     /locus_tag="VEL74_06085"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HYG62130.1"
                     /translation="MTSRLHVAIIMDGNGRWAVSRGRPRTAGHVAGAEAVRRTVEAAR
                     DQGIGTLTLFAFSSDNWRRPDGEVRALMRLFRRYLVTEAERCAENGVRIHVIGRRDRL
                     PPRLLETIEAAEAATAGAGPFRLRIAVDYSARDTILRAASRLGRLGRPEDLSREAFAR
                     FLAEPPHDGGPVPDVDLLIRTGGEKRLSDFLLWECAYAELVFTDRMWPDFGAADLAEA
                     VREFHSRERRFGKVTATPASAAAPAPLQRETRRAGLAG"
     gene            complement(1771..2175)
                     /locus_tag="VEL74_06090"
     CDS             complement(1771..2175)
                     /locus_tag="VEL74_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HYG62131.1"
                     /translation="MARAKRVPHEPGGPAGGPADGEPEERAGMGEGLAQQAALDLDRL
                     IHERIRLGIVSALAVNEHLTFNDLKRLLKTTDGNLSVHARKLEEAGYVTCAKYFEGRV
                     PKTEYRLTPSGRRALERYLDHMEAIIHATRLG"
     gene            complement(2168..2800)
                     /locus_tag="VEL74_06095"
     CDS             complement(2168..2800)
                     /locus_tag="VEL74_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62132.1"
                     /translation="MIEPRPNRPRMAEPPPALHLRALDHLRFIRETMEGAACFTGVSG
                     VGEVAVGLTALVAAWIAARQGSATAWLLTWLTEAVVAVLITAGAMVWKARRTGATLLS
                     GTVRRFALALFAPLVSGALLTFVLFFQGSVAVLPGLWLLLYGTGVVTGGAYAVRTVPV
                     MGMAFMALGVVALAAVVFAAPPVWSDLLMAAGFGGLHIVFGAIIAWRHGG"
     gene            complement(3380..3835)
                     /locus_tag="VEL74_06100"
     CDS             complement(3380..3835)
                     /locus_tag="VEL74_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HYG62133.1"
                     /translation="MPEMPDKLANTLEALSLIGDRAERIQMLIDIAERFREVPPRVAA
                     RPFPEEHLVPACESEAYVWAESRPDGTLDFHFAVENPQGISAKAMAVILGDTLSGQPP
                     EQVAEVPQDVVYRVFGRELSMGKSMGLMGMVSMVRNLARKHADARRSAS"
     gene            complement(3860..4723)
                     /locus_tag="VEL74_06105"
     CDS             complement(3860..4723)
                     /locus_tag="VEL74_06105"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HYG62134.1"
                     /translation="MSQIEDRGYAHPEALVSTDWVAGHLNDPSVRIVESNEDPLLYAS
                     GHIPGAVEVDWTRDLNDPVRRDYLDREGFEALMRRIGATRDTTVVFYGDRNNWWATYA
                     FWVFQLFGHDRARIMDGGRIKWVNEGRPVTREVPAYPASDYRAPERDDSRVRAFRDEV
                     LRHVESRRPLIDVRSPAEFTGERLHMPEYPNEGALRGGHIPGAANIPWARAVDAEDGT
                     FKTAEELRALYEGEARLQPQDDVVVYCRIGERSSHTWFVLTHLLGYERVRNYDGSWTE
                     WGNLVGVPIEK"
     gene            4948..5268
                     /locus_tag="VEL74_06110"
     CDS             4948..5268
                     /locus_tag="VEL74_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62135.1"
                     /translation="MFRSFLYRGVILSGVVCLCLLPLAEAHAVPAERGGGVGAAVERT
                     WSAEWAEAVRGLLAGLWGKDSAERDRTVEVDPPNGKGDGTGIDPHGGPRPRQSQRPQQ
                     VAEP"
     gene            5300..6718
                     /locus_tag="VEL74_06115"
     CDS             5300..6718
                     /locus_tag="VEL74_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYG62136.1"
                     /translation="MSELHPDREALQRFLSDALSEPESRALQRHLLACPPCEERLIRL
                     LPREGGETAAPGAPSGPPPAPPEAGYQRLVRRVLAETGLEIERRWSLLAAERVSAEEL
                     WRELRLESRERRHALVLGDPRFQSWGLFELLIGKSDHAVLVEPQKALEILRLALDLTG
                     HLGATSHGPGAVDAAKARAWACLGNALRILSDFRQSEQAFQTAEAHLAKSWLDPMDEA
                     LVLELKASLRRAQRRFGEALVMLEAAIGLYREINEPHLQGRALLKKGLVLQYGHRFEE
                     ACACFRSSLFLLDGTEDPRLVAVGQFNLINCLHDAGRTAEAAALLPEGRQMMRVSGTR
                     SDLVRLRWLEGKIAAVLGPPAEAERALLEARDALVEDAAAFDAALVSLDLAGLYAREG
                     RAAETKRLIQEILPIFRSCEVHGEALAALIVFQQAAEMEQLSLGLVEEIASFLQQAKS
                     NPGLRFRDGGEGLEAGDGAAFE"
     gene            complement(6949..7503)
                     /locus_tag="VEL74_06120"
     CDS             complement(6949..7503)
                     /locus_tag="VEL74_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019607948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HYG62137.1"
                     /translation="MRDPRETLIGWLNDAYAMEQGLVQVLENHANDAKDMPEAYRKLT
                     QHLEQTRMHAERVRECVERLGGSTSTMKTAMGTVSGFFQGRSTGASPDELVKNALADY
                     ASEHLEIASYRALIVAAQALGENHVVQVCEQILREEEEMASWLERQLPMIVQDYLGQG
                     ATVGAGMGTASGGSFSGTPGGNRF"
     gene            complement(7616..8470)
                     /locus_tag="VEL74_06125"
     CDS             complement(7616..8470)
                     /locus_tag="VEL74_06125"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HYG62138.1"
                     /translation="MPEEAKDRPGTLIIIGGHEDREGEKKILRAVADRVGKGTLVVST
                     VGTNSPDEVWEEYRKAFRDLGVERIEHLDVDDREQALDPERARVVEEATVVFFTGGDQ
                     LKITSQLGDSQTYQNIEKLYFERKGTVAGTSAGASVMSETMLVSGNGEESHKVDAMLR
                     MAPGLGLIQDMVIDQHFAERGRIGRLLAAVAQNPRHLGVGIDEDTAIIVEGGEAFEVI
                     GSRAVYVVDGAHVSYSNLGEQEEDRDKVMAAFDYKLHVLSEGNRYDLVERRPEVPGKP
                     EVKEKKSA"
     gene            complement(8492..9646)
                     /gene="iadA"
                     /locus_tag="VEL74_06130"
     CDS             complement(8492..9646)
                     /gene="iadA"
                     /locus_tag="VEL74_06130"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HYG62139.1"
                     /translation="MLLLIENGEVYAPEPRGRQAVLVWDGKVVRMGGIDSRGAREAAE
                     TLGGELEVIDASGCLVVPGFVDPHMHLTGGSGEEGFASRTPDLQLTEIVPWGTTTVVG
                     VLGVDATTRTLPGLLAKVRGLNEEGMTAFLYTGHYEVPPVTFTDSVRNDLLLIREVIG
                     VGEIAISDHRAVQPDVRELARLVVDAHVGGMLSGKAGITHFHLGEIPDRLSPLRALID
                     DLHVAPELLYPSHVQRTPELLAEAVELAKRGSFVDMDSAEEGLGGCIRSFLDQGGPAA
                     RLTLSSDADSTAPKRLFEQVRAAVLEDGLPLEQVLPLVTANPACILKLQGKGKIEPGA
                     DADLVVLRKESLEIVDVIARGKLMVRRGEVVQRESYLEESDRILVLEGKK"
     gene            complement(9757..10128)
                     /locus_tag="VEL74_06135"
     CDS             complement(9757..10128)
                     /locus_tag="VEL74_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62140.1"
                     /translation="MRIIPTKVHGVIDYTWGALLMASPWLFNYARNEEETVVAVAMGA
                     GSIAYSLVTDYEMGAVPMLSMRTHLLIDGAAGLLLAASPWLLRFDDFVRAPHVAFGLL
                     AVVTASLTETRARRRQLPARA"
     gene            complement(10191..10715)
                     /gene="raiA"
                     /locus_tag="VEL74_06140"
     CDS             complement(10191..10715)
                     /gene="raiA"
                     /locus_tag="VEL74_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HYG62141.1"
                     /translation="MEISTRLIELSPPLEAELRRRAAKLERYYDRITSCRIAVERESN
                     HHQEGGPYRVRVDITVPGSELVANKAAEDLNTAVRAAFSAAERQVDDFSRRRRGEVKT
                     PVGPPHGTVARIFAEEGFGFILAEDGREIYFHRNAVLDDFDSLQPGAPVRFAESQGDE
                     GPQASTVSLIGRED"
     gene            10874..11341
                     /locus_tag="VEL74_06145"
     CDS             10874..11341
                     /locus_tag="VEL74_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62142.1"
                     /translation="MAQTSNPELRTMLGVRWHEQAEAPASARPLYLLAWVQGIFYALT
                     GLWPLLHMPSFLAVTGPKVDLWLVKTVGLLILVIGAVLLVGARRRSIGPELALLAAGA
                     AAALAGVDLVYALSDRISDIYLLDAAAEIGLVILWIASWWRTAARRKGSRRLA"
     gene            complement(11351..>11743)
                     /locus_tag="VEL74_06150"
     CDS             complement(11351..>11743)
                     /locus_tag="VEL74_06150"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62143.1"
                     /translation="YRRVVAVEGDRIGARYARLNIRRNQAENLEALNLSVETWIQESL
                     PDRVDRVVVDPPRSGLSGAIRRHLLERLPERLTYVSCHPAALARDLRLLSRSYRLESL
                     VLFDLFPQTGHMEAVVQMVRTEEAPAVL"
BASE COUNT         1771 a         4194 c         3996 g         1782 t
ORIGIN      
        1 cgtcgactcg cccgacctgg cgctcaacgt ctcgcgcgag atgctgcagc aggaccgcca
       61 catcaccctg atccgcaagg gcctcaccaa gaaggtcctc gacgtcctcg acgagatgcg
      121 cgacaaggag ccggacacct acctcaactt ctggaaggag ttcggccgcg ccatcaagga
      181 gggggtgagc tccgactacg agaacaagga gaagctcctg gagctcctgg tcttccagtc
      241 gtccaaccac ccggagaagc tgacgacgct gcgcgagtac gtgtcgcgga tgaaggagga
      301 ccagaaggag atcttctacc tcaccggcga ttcccgggcg caggtggaga actcacccca
      361 cctcgaggcg ttgaaggaca aggggatcga ggtcctctac ctgatcgaca cggtggacga
      421 gctgctcacc cagtcgctcc acgagtacga cggcaagaag ctgaagtcgg taggcaaggg
      481 cacggtcaac ctcggcaacg aggaggagaa gaagcagacc gaggaggcgc tcaaggccaa
      541 ggaggaggag ctcaagccgc tgctcgacgc catgcagcag aagctcgaca cctgggtcaa
      601 gcaggtgcgc ctcaccaacc gcctcaccac ttcgccggtc tgcctggtgg gcgccgagca
      661 cgactacagc ccgcagctcg agaagctcct gcagaagggc aaggggggcg gccccaagca
      721 gcgccggatc atggagctca accccaacca ccagatcctg ggccggttga aggagcgctt
      781 cgagcagaac gccgaggacc cgctggtgga ggactacgcc cagctcctct tcggctacgg
      841 cctgatctcc gagggcgccg agctcgagga cccggtgcgc ttcaaccgcg ccatggccga
      901 cctgatgacg cagaacctcg gcacgccggc cgcctgaggc ggccgccgat ccgggtgacc
      961 ctccgcccgc cgctccggcc gccctacccg gcgagcccgg cgcggcgggt ttctctttgg
     1021 agcggagcgg gagcggcggc gctggcgggg gtggcggtca ccttgccgaa gcggcgctcc
     1081 cgggagtgga actcccgcac cgcctcggcc aggtcggcgg cgccgaagtc gggccacatg
     1141 cggtcggtga agaccagttc ggcgtaggcg cactcccaga gcaggaagtc ggacagccgc
     1201 ttctcgccgc cggtgcggat gaggaggtcg acgtcgggca ccggcccgcc gtcgtggggc
     1261 ggctcggcga ggaagcgggc gaacgcctcg cgcgacaggt cctcgggacg gccgaggcgg
     1321 cccaggcgcg acgcggcgcg caggatggtg tcgcgcgccg agtagtcgac ggcgatgcgc
     1381 agccggaagg gcccggcgcc ggcggtcgcc gcctcggccg cctcgatggt ctccagcagc
     1441 cgcgggggga ggcggtcccg ccggccgatg acgtggatgc gcacgccgtt ctccgcgcaa
     1501 cgctccgcct cggtgaccag atagcgccgg aacaggcgca tgagcgcccg cacctcgccg
     1561 tcaggccggc gccagttgtc ggacgagaag gcgaacaggg tcagcgtgcc gatcccctgg
     1621 tcgcgcgccg cctccacggt gcggcgcacc gcctcggcgc cggccacgtg gccggcggtc
     1681 cgcggccgtc cccgggagac ggcccagcgg ccgttgccgt ccatgatgat cgccacatga
     1741 agcctgctgg tcatcgctct tctcctggga tcaaccgagc cgggtggcgt ggatgatggc
     1801 ttccatgtgg tcgagatagc gctcgagggc gcgccggcct gaaggggtca gccggtactc
     1861 cgtcttcggc acccgcccct cgaaatactt cgcgcaggtg acgtagccgg cttcctcgag
     1921 cttccgggcg tggacgctca ggttgccgtc ggtggtcttg agcagccgct tgaggtcgtt
     1981 gaaggtcagg tgctcgttga ccgccagcgc gctcacgatg ccgaggcgga tccgctcgtg
     2041 gatgaggcgg tcgaggtcga gcgccgcctg ctgggccaga ccttctccca taccggcgcg
     2101 ctcctcgggc tccccgtcgg cgggaccacc cgcggggccg ccgggctcgt ggggcactcg
     2161 cttcgcccta gccaccgtgc ctccaggcga tgatcgcccc gaagacgatg tggaggccgc
     2221 cgaagccggc ggccatcagg aggtcgctcc acaccggcgg cgcggcgaag accaccgccg
     2281 ccagcgccac cacccccagc gccatgaacg ccatgcccat gaccggcacg gtccgcaccg
     2341 cgtaggcgcc gccggtcacc acaccggtac cgtagagcag cagccagagg ccggggagca
     2401 ccgccaccga gccctggaag aagaggacga aggtcagcaa cgccccggaa accagcggcg
     2461 cgaacagcgc cagggcgaac cggcggaccg tcccggagag cagcgtggcg ccggtccggc
     2521 gggccttcca gaccatcgcg ccggcggtga tgagcaccgc caccacggcc tcggtcagcc
     2581 aggtcagcag ccacgccgtg gcgctcccct ggcgcgcggc gatccaggcc gccaccagcg
     2641 cggtcagccc caccgccacc tcccccactc ccgacacgcc ggtgaagcag gccgccccct
     2701 ccatggtctc gcggatgaac cgcaggtggt ccagcgcccg cagatgaagg gcggggggcg
     2761 gctcggccat ccgggggcgg ttggggcggg gctcgatcac gggaacacca tactcccgcg
     2821 gcacccagcc cgtcaagtac ttttcggcgc aaagtcgagc agccccgccg cgacggcgcc
     2881 cgcgacctcg cccgcggacc acgccctctc caccccagcc cggaaccctc cgccttcagg
     2941 ccggagccgt cctcacggat tccgcggccg tccgccttca gccgggagcc gggagccgct
     3001 cgcggctgga aatgagccgt cccaccctgg aacggaacgg ctcgccttca gcaagaagcc
     3061 gtccatccct ggactggagc ggctcccatt cagcatggag ccgtcgctac aggttcagga
     3121 gccttcccgg ctcagggtgg acatctccat ttccagacgg agaggctcca ccgtggaaat
     3181 ggaggcctcc accctggaat ggagaccctc caccgtgagt gggagaggcg ccgcttcagg
     3241 gtgggacctc tccggtccag ggcggggagc ctccattcca gggtgggacc tcgccgcttc
     3301 agggtgaggc ctctcctgct caggaatgga cggctcccat tcagccgggg acggctccag
     3361 aacaggccga tcggggttgc taggaggcgg accgccgggc gtcggcgtgc ttgcgggcca
     3421 ggttcctgac catggagacc atgcccatga gccccatcga cttgcccatg gagagctcgc
     3481 ggccgaagac ccggtagacg acgtcctgcg gcacctcggc cacctgctcg ggcggctggc
     3541 ccgagagggt gtcgccgagg atcacggcca tcgccttggc cgagatcccc tgcgggttct
     3601 cgaccgcgaa gtggaagtcc agggtgccgt ccggccgcga ctccgcccag acgtaggcct
     3661 cggactcgca ggccgggacc agatgctcct cggggaaagg gcgggcggcc acccgcggcg
     3721 gcacctcgcg gaaccgctcc gcgatgtcga tcagcatctg gatgcgctcg gcgcggtcgc
     3781 cgatcaggga gagcgcctcc agggtgttcg ccagcttgtc gggcatctcg ggcatcgtcg
     3841 cctcggctcc tctgctgctt tacttctcga tgggcactcc gacgaggttg ccccactcgg
     3901 tccagctccc atcatagttg cggacccgct cgtatcccag caaatgggtg aggacgaacc
     3961 aggtgtggct gctccgctcg ccgatgcggc agtagacgac gacgtcgtcc tgcggctgga
     4021 gccgggcctc cccctcgtag agggcgcgca gctcctcggc ggtcttgaag gtgccgtcct
     4081 cggcgtcgac ggcacgcgcc caggggatgt tggccgcccc cgggatgtgg ccgccgcgca
     4141 gcgccccctc gttcgggtac tccggcatgt gcagccgctc gccggtgaac tcggcggggc
     4201 tgcgcacgtc gatcagcggc cgccgcgact cgacgtggcg gagcacctcg tcgcggaagg
     4261 cgcggacccg ggagtcgtcc cgctcgggcg cccggtagtc gctggccgga taggccggca
     4321 cctccctggt caccggccgc ccctcgttga cccacttgat gcggccgccg tccatgatcc
     4381 gggcccggtc atggccgaag agctggaaga cccagaaggc gtacgtggcc caccagttgt
     4441 tgcggtcgcc gtagaagacc acggtggtgt cgcgggtggc gccgatccgg cgcatcagcg
     4501 cctcgaagcc ctcacggtcc agatagtcgc ggcgcaccgg gtcgttcagg tcgcgggtcc
     4561 agtccacctc caccgcgccc gggatgtggc cgctggcata gagcagcgga tcttcgttgg
     4621 actccacgat ccgcaccgac ggatcgttca ggtgcccggc gacccagtcg gtcgacacca
     4681 gggcctcggg atgggcataa ccgcggtctt cgatctggct catggcgggg cttctctcct
     4741 ttccttctcc tccccaactg tgaccaacgt tatcaaagaa gccgtcaccc tgccccaggc
     4801 cttccccctt ccccggcggc gcgatctgcg gtactctcgc cacaattctt caacaggcgt
     4861 tggatggctc ccctctcggg agcagaccgt ttacggcagg cggtgcagcc gtggtggccg
     4921 ctcgtgacct gccgggaagg agtctcgatg tttcgcagtt tcctgtatcg tggagtgatc
     4981 ctgtccgggg tggtatgttt gtgtctgctg ccgctggccg aagcgcacgc cgttccggcc
     5041 gagcgcggcg ggggcgttgg cgccgccgtc gagcggacct ggagcgccga gtgggccgag
     5101 gcggtccgcg ggctcctcgc cggcctctgg gggaaggact ccgcggagag ggaccggacc
     5161 gtggaggtgg atccgccgaa cggcaaaggg gatggcacgg ggatcgatcc ccacggcggc
     5221 ccccggccgc gccagagcca acgcccccaa caagtagccg aaccgtgacc gggccgtttc
     5281 cggcaaggag cctcgaacga tgagcgagct tcacccggac cgcgaagcct tgcagcgatt
     5341 cctctccgac gcgttgagcg agccggagag ccgcgccctg caacgccacc tcctcgcctg
     5401 ccccccttgt gaggagcggt tgatccggct cctgccgcgg gagggcgggg agacggcggc
     5461 gcccggcgcg ccctccggtc cgccgccggc gccgcccgag gccggttacc agcggctagt
     5521 ccggcgcgtc ctcgcggaga ccgggctaga gatcgagcgc cgctggtccc tgctggccgc
     5581 cgagcgggtg tcggcggagg agctctggcg cgagctgcgc ctggagagcc gggaacggcg
     5641 ccacgccctc gtcctcggcg acccccggtt tcagagctgg ggcctcttcg agctgctgat
     5701 cgggaagtcc gaccacgccg tgctggtgga gccgcagaag gcgctcgaga tcctgcgcct
     5761 ggcgctcgac ctgaccggcc acctcggcgc caccagccac gggccgggtg cggtggacgc
     5821 cgccaaggcg cgggcctggg cctgcctcgg caacgcgctg cgcatcctct ccgacttccg
     5881 gcagtcggag caggctttcc agaccgccga ggcccacctg gccaagagct ggctcgatcc
     5941 catggacgag gccctggtgc tcgagctcaa ggcgtcgctg cgccgggcgc agcgccggtt
     6001 cggcgaggcc ctcgtcatgc tggaagcggc catcggcctc taccgcgaga tcaacgagcc
     6061 gcacctccag ggccgggcgc tgctcaagaa ggggctggtg ctccagtacg gccaccgctt
     6121 cgaggaggcc tgcgcctgct tccgcagcag cctgttcctg ctcgacggca ccgaggaccc
     6181 ccggctggtg gcggtggggc agttcaacct catcaactgc ctccatgacg ccggacggac
     6241 cgccgaggcg gcggccctgc tgcccgaggg gcgccagatg atgcgcgttt ccggcacccg
     6301 ctcggacctg gtgcggctgc gctggctcga aggcaagatc gccgccgtcc tcgggccgcc
     6361 ggccgaggcc gagcgggccc tcctcgaggc gcgcgacgcg ctggtcgagg acgccgccgc
     6421 cttcgacgcc gcgctcgtct ccctggacct cgccggcctc tacgcgcggg agggccgggc
     6481 cgccgagacc aagcgcctga tccaggagat cctccccatc ttccggtcct gcgaggtgca
     6541 cggcgaggcg ctcgccgccc tcatcgtctt ccagcaggcg gccgagatgg agcagctcag
     6601 cctcgggctg gtcgaggaga tcgccagctt cctgcagcag gcgaagagca atcccggcct
     6661 gcgcttccgc gacggcggcg aggggctgga ggcgggagac ggcgccgcct tcgagtaact
     6721 ctggcagatt ttttgctaat cagtctgaac gggagagaga gcggggagga gagaggatga
     6781 caggccgttc accctggttg gcgctccgag tctgccacgg cctgccatcc tctctgttta
     6841 tggccgtgat tcctcagatc ccgacgaaat ccccctgaag caggaagggc ccggccgccc
     6901 cctcccgcag gaggatggag cgagccgagc ccggaccttg ccggaaggct agaagcggtt
     6961 gccgcccggc gtgcccgaga agctgccgcc actggcggta cccatcccgg cgccgacggt
     7021 ggcaccctgg cccaggtagt cctggacgat catcgggagc tgccgctcga gccagctcgc
     7081 catctcctcc tcctcgcgca ggatctgctc gcagacctgg acgacgtggt tctcacccag
     7141 cgcctgggcc gccacgatca gcgcccggta gctggcgatc tcgaggtgct cggaggcgta
     7201 gtccgccagg gcgttcttga ccagctcgtc gggcgacgcg ccggtggacc ggccctggaa
     7261 gaagccggag acggtgccca tggcggtctt catcgtcgag gtgctgcccc ccagccgctc
     7321 cacacactcg cgcacccgct cggcgtgcat gcgggtctgc tccagatgct gggtcagctt
     7381 gcggtacgcc tcgggcatgt ccttggcgtc gttggcgtgg ttctcgagga cctggaccag
     7441 gccctgctcc atggcatagg cgtcgttgag ccagccaatc agcgtctcgc gtggatctct
     7501 catcgccata ttgcaactcc ttccgttgat ctttcttgtt gtctgaccgg gcgcgcggta
     7561 cttccaggcg ccagagaaag aaggttgcaa catgcgtgcc gattggaaag atttttcacg
     7621 cgctcttctt ctccttgacc tcgggcttgc cgggcacctc cggccggcgc tccacgaggt
     7681 cgtaccggtt gccctcggag agcacgtgga gcttgtagtc gaaggccgcc ataaccttgt
     7741 cccggtcctc ctcctgctcg cccaggttcg agtaggagac gtgggcgccg tccacgacgt
     7801 agaccgccct tgagccgatc acttcgaacg cctcgccccc ctcgacgatg atggcggtgt
     7861 cctcgtcgat ccccaccccg aggtggcgcg ggttctgggc taccgcagcg aggaggcggc
     7921 cgatccgccc acgctcggcg aagtgctgat cgatcaccat gtcctggatg agcccgagac
     7981 cgggagccat gcgcagcatg gcgtccacct tgtgcgactc ctcgccgttg ccggagacca
     8041 acatggtctc gctcattacc gaggccccgg ccgaggtgcc ggcgaccgta cccttcctct
     8101 cgaaataaag cttctcgatg ttctgatagg tctgcgagtc gccgagctgg ctggtgatct
     8161 tcagctggtc gccgccggtg aagaagacca cggtcgcctc ttcgacgacg cgcgcacgct
     8221 ccgggtcgag cgcctgctcc cggtcgtcga cgtccaggtg ctcgatccgt tcgacgccca
     8281 gatccctgaa cgccttccgg tactcctccc agacctcgtc gggcgagttg gtgcccaccg
     8341 tcgacaccac cagcgtgccc ttgccgaccc ggtcggccac ggcgcgcagg atcttcttct
     8401 ctccttcccg gtcctcgtgc ccaccgatga tgatcagcgt tcccggccgg tccttcgctt
     8461 cctcaggcat tctctcccct cctcgagtcg ttcacttctt tccttccagc accaggatcc
     8521 ggtcgctctc ttcgaggtag ctctcgcgct ggacgacctc gccccgccgg accatcagct
     8581 tgccgcgggc gatcacgtcc acgatctcca gcgactcctt gcggaggacc accaggtcgg
     8641 cgtccgcccc cggctcaatc ttccccttgc cctgcagctt caagatacac gccgggttgg
     8701 cggtgaccag cggcaggacc tgctcgagcg gcaggccgtc ctcgagcacc gccgcgcgca
     8761 cctgctcgaa cagccgcttc ggcgccgtgc tgtcagcgtc cgaagagagg gtcagccgcg
     8821 ccgccggccc cccctggtcg aggaagctgc ggatgcagcc gcccagcccc tcctcggcgg
     8881 agtccatgtc gacgaacgag ccgcgcttcg ccagctcgac cgcctccgcc agcagctccg
     8941 gggtacgctg gacgtgggat ggatagagca gctccggcgc gacgtggaga tcgtcgatga
     9001 gcgcccgcag gggggagagc cggtccggga tctcccccag atggaagtgg gtgatccccg
     9061 ccttgccgga cagcatgccg ccgacgtggg cgtcgaccac cagccgcgcc agctcccgca
     9121 cgtcgggctg gaccgcgcgg tggtctgaga tggcgatctc gccgacgccg atcacctccc
     9181 ggatgagcag gaggtcgttg cgcacggagt cggtgaaggt caccggcggc acctcgtagt
     9241 ggccggtata gaggaaggcc gtcatcccct cctcgttcag cccccgcacc ttggcgagca
     9301 ggcccggcag cgtgcgggtg gtggcatcga cgccgagcac cccgacgacg gtggtcgtcc
     9361 cccaggggac gatctcggtg agctggaggt cgggcgtgcg gctggcgaag ccctcctcgc
     9421 cgctgccgcc ggtcagatgc atgtgcggat cgacgaagcc cggcaccacc aggcagccgg
     9481 aggcgtcgat cacctcgagc tcccccccca gggtctccgc cgcttcgcgg gctccccggg
     9541 agtcgatccc gcccatccgg acgactttgc cgtcccagac cagcaccgcc tgccggccgc
     9601 ggggctccgg cgcgtagacc tcaccgttct cgatcagtaa cagcatggct ctctcccgca
     9661 agactcagaa aaaaggccgg agccggtccg gcgagacgag tccccggagg aagaagggct
     9721 cgacagcccg gaccggctcc gagagcgatt ccaggatcag gcccgcgccg gcagttgccg
     9781 gcggcgggcc cgggtctcgg tcagggacgc ggtcaccacg gccagaagcc cgaaggcgac
     9841 gtgcggggcg cggacgaagt cgtcgaaccg cagcagccac ggcgaggcgg cgagcagcag
     9901 gccggcggcc ccgtcgatca gcagatgggt ccgcatcgac agcatgggga ccgcccccat
     9961 ctcgtagtcg gtcacgaggc tgtaggcgat ggaacctgct cccatcgcca ccgcgacgac
    10021 ggtctcctcc tcgttgcggg catagttgaa gagccacggg gacgccatga gcagcgcccc
    10081 ccaggtgtag tcgatgacgc cgtgaacctt ggtggggatg atcctcatgg tgcactcctt
    10141 tgccagccgt ccccgtcctg tattccttct attgccttcg tgaacccttt tcagtcctcc
    10201 cggccgatca ggctgaccgt gctcgcctgc ggcccttcgt ccccctgcga ttcggcgaag
    10261 cgcaccggcg cgccgggctg gaggctgtcg aagtcgtcga gcacggcgtt gcgatggaag
    10321 tagatctcgc ggccgtcctc cgccaggatg aagccgaagc cctcctcggc gaagatgcgg
    10381 gccaccgtcc cgtgaggggg gcccaccggg gtcttcacct cgccccggcg gcggcgcgag
    10441 aagtcgtcca cctggcgctc ggcggcgctg aaggccgcac gcacggcggt gttcagatcc
    10501 tccgccgcct tgttcgccac cagctccgag ccgggcacgg tgatgtcgac gcgcacccga
    10561 tagggcccgc cttcctgatg gtggttgctc tcgcgctcga cggcgatgcg gcagctcgtg
    10621 atccggtcgt agtagcgctc gagcttggcg gcgcggcgcc gcagctccgc ttccagcggg
    10681 ggggagagct cgatcagccg ggtagaaatt tccaagggta cctgcatgca gaggctcctt
    10741 tccgtgggag gagaccagtc ctctgccggg caccggcctc ccgtcacccg ggttcagcgc
    10801 aagcgccatg ccagcgcgcg gggttccgtc cgccggagtc cttctggcac gtgaagtgca
    10861 gcgatcgaga aaaatggcac aaacctcgaa ccccgagctg cggacgatgc tgggtgtccg
    10921 ctggcatgaa caggcggagg cccctgcatc ggcccgcccc ctatacctcc tggcctgggt
    10981 ccaggggatc ttctatgcgc tcaccggcct ctggccgctg ctccacatgc cgagcttcct
    11041 ggcggtcacc ggccccaagg tagacctctg gctggtgaag acggtggggc tgctcatcct
    11101 ggtgatcggc gcggtgctgc tggtgggggc ccgccgccgg agcatcggac ccgagctggc
    11161 gctgctcgcc gccggcgccg ccgcggccct ggcgggcgtc gacctggtct acgccctctc
    11221 ggaccgtatc tccgacatct atctattgga tgctgcggcc gagatcggcc tggtgatcct
    11281 gtggatcgcc tcctggtggc ggacggccgc caggaggaag ggctcccggc ggctcgccta
    11341 gcggaacgcc tcagaggacg gccggcgcct cctcggtccg caccatctgg accaccgcct
    11401 ccatgtgccc ggtctgcggg aacaggtcga acagcaccag gctctccagc cggtacgagc
    11461 gggagagcag ccgcaggtcg cgcgccaggg cagcggggtg gcaggagacg taggtcagcc
    11521 gctccggcag gcgctccagg aggtgccggc ggatcgcccc cgagagcccg gaccgcggcg
    11581 ggtccaccac cacccggtcg acccggtccg gcaggctctc ctggatccag gtctccaccg
    11641 agaggttgag ggcctccagg ttctcggcct ggttgcggcg gatgttgagc cgcgcgtagc
    11701 gcgcgccgat gcggtccccc tcgaccgcca ccacccgccg gta
//
LOCUS       DASPJI010000232         5198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_476268, whole genome shotgun sequence.
ACCESSION   DASPJI010000232 DASPJI010000000
VERSION     DASPJI010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476268"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..598)
                     /locus_tag="VEL74_06155"
     CDS             complement(182..598)
                     /locus_tag="VEL74_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62144.1"
                     /translation="MADDEAYWALRLLETSIQASGLSERQLEKRLGWEKGALGKVLRN
                     GGALEHQQILDILEVLSRDGREGGARGAKGSPPAGPLVGTLLERFHRLGIPASETAFV
                     GPPSPPPLQGAELERRVEDLLAEAFGHLEEIQDEED"
     gene            716..1966
                     /locus_tag="VEL74_06160"
     CDS             716..1966
                     /locus_tag="VEL74_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62145.1"
                     /translation="MPDIRPDTRSDTQPGDRIQKALKLLADEVRERIEHHPMGHLVAG
                     RGEKLDLRLPVPTALRDGQLEKLSAVAAEAVQDAIQALLSHTAVFQPGRVFCLRCQSA
                     TCEHSAPAGSREVFIGYGGTGTPRFADFGQWLLRLRDPRVDLLYREPPQLLTIVATGE
                     ELEGELLPMFRQREADYRIHGQVAAGWYQVPAPVTGNGGGHRQPLAVTFQIVSSQPRG
                     HRRRFGINVLGIGPGGEPLEHLFDRLGQIPWTDPVRWAQSVLAGIESQLARSPRMPRE
                     AIDKRLDGLVQGLARRLDKGWRGRERRTHHAEQRHSEGDRPTRMALADLARATPENLL
                     FDTRNQTLVVVGERGRAHVFSLEGKLVTSVRYNPASIERRRDKGIWRRAAAAEVETVR
                     LRLQKAGDGQGAAEGVSGSAVSSG"
     gene            complement(1918..2466)
                     /locus_tag="VEL74_06165"
     CDS             complement(1918..2466)
                     /locus_tag="VEL74_06165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYG62146.1"
                     /translation="MSWRRVIARLTALVVLAVLLFLAYPYLRHVTRVVQLLRQPAPSW
                     LPVPVEGVEPGDLGDTWGASRSGGRTHEGIDIFAPRGTPVASATRGIVVRKGWNRLGG
                     RTVSVLGPGGRYHYYAHLDEWDAPEPGDWVEAGTVLGYVGDSGNAAGTPTHLHYGIYG
                     GGEALNPYPLLTAEPDTPSAAP"
     gene            complement(2468..2809)
                     /locus_tag="VEL74_06170"
     CDS             complement(2468..2809)
                     /locus_tag="VEL74_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03792 family protein"
                     /protein_id="HYG62147.1"
                     /translation="MVVEWMRVRVAPGARDRYVEVDTEVWTPGLSAEAGFLGKEVWLG
                     EDETDLVLVIRWRSEEEWKGIEPARLEELERRFRDLFGEPFQVVETRSYRPVHEVKAG
                     VADRSIRSRWR"
     gene            complement(2822..3598)
                     /locus_tag="VEL74_06175"
     CDS             complement(2822..3598)
                     /locus_tag="VEL74_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005485050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HYG62148.1"
                     /translation="MPVLIGTSGWQYRHWDGSFYPSEVPKSAQLPWYAERFLTVETNA
                     TFYRLPEAGVFRAWAGQTPGDFLFAVKASRFLTHMKRLKDPEEPIERLMSRARELGAK
                     LGPVLLQFPPQMHRNAERLDAALALLCPQARVAVEPRHESWFVDEVREILARHGAALC
                     IADRGAHLLTPEWRTADWGYLRFHGGEAHPPGCYTRETLAARARLAADLWGPDAEVWA
                     FFNNDFHRCALRDAGWFAEAAAAAGLAPTRVPDPATVAVG"
     gene            3585..4202
                     /locus_tag="VEL74_06180"
     CDS             3585..4202
                     /locus_tag="VEL74_06180"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYG62149.1"
                     /translation="MSTGMPFLMILLLLLGPPPVAPAEGSSAELRRLAAGEVLVTLAP
                     VSPAGGPREGIGRGVIDAPPDRVYRALTDWAHAEEWMPFVERSDAAPQPDGSVVSFQA
                     LDLPAPLGDRHYKIRARAGVASRKTRVWRTRWSYVPGSGNVEDHRGSWELTAFGDGRT
                     LATCRLYTDPGGAVAAWMMDRATRKSLPWIFDGLRQQVRRSRYDG"
     gene            complement(4271..>5198)
                     /locus_tag="VEL74_06185"
     CDS             complement(4271..>5198)
                     /locus_tag="VEL74_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015149445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HYG62150.1"
                     /translation="RPAVPGVSLAAWAEANRPTIDAALAKHGGILFRGFDIPRVEDFD
                     RTVQAISGDLLEYKERSSPRHAVAGKIYTSTDYPPSEPIFLHNENSYQKTWPMRIFFF
                     CNVQPGEGGETPIADTRRVYQRIDPTVRQKFAEKGWMYVRNFGDGFGLPWKEVFQTDD
                     KAAVEAHCQKSGIQVEWKEGDRLRTRAVRRALTAHPQSGEMVWFNHATFFNVSTLTPR
                     VREALQEEFAEEDLPTNTYYGDGTPIEPEVLEHLRECYNAETVAFPWQKGDLLMLDNM
                     MVAHGRAPFSGQRQILVGMSHAVTWDDMPAPQ"
BASE COUNT          731 a         1907 c         1793 g          767 t
ORIGIN      
        1 gtgacgtctc ttcaagatcg atctggcggg gcaacattgc caggagggat tcccactgcg
       61 ttttaaggat cgccaaaggt tccatggcac gagattatag gacctcgtgc catgaagacg
      121 ctaagttagc gcctatgggg ctcgcccctc ccctacattt ccatacccat ttctctttct
      181 ttcaatcttc ctcgtcctgg atctcctcga ggtggccgaa ggcctcggcc aggagatcct
      241 cgacccggcg ctccagctcg gcgccctgta gagggggcgg ggatggcggc cccacgaagg
      301 cggtctccga agcgggaatg ccgagccggt ggaagcgctc gagcagcgtc cccaccagcg
      361 gtcccgccgg ggggctcccc ttcgcccctc gcgcccctcc ctcccttccg tcgcggctca
      421 ggacctccag gatgtcgagg atctgctggt gctccagagc acccccgttg cgcagaacct
      481 tccccagggc gcccttttcc cagccgagcc gcttctccag ttgccgctcc gacaggccgg
      541 aggcctggat cgacgtctcg agcaacctta gcgcccagta ggcctcgtcg tccgccatat
      601 cgaggattct aaacggttga tatctcgaat gggcggcctt cgccgccgcc ccggtggaat
      661 cgtggaatac ctctttcctc gccagcgctt acactcttgc gcccgaggag cccgcatgcc
      721 cgacatccgc cccgatactc gctccgacac ccagcctggc gaccgcatcc aaaaggccct
      781 gaagctcctc gccgacgagg tccgcgagcg catcgagcac caccccatgg gacatctggt
      841 ggccggccgc ggcgagaagc tcgacctgcg cctgccggtc cccaccgccc tgcgcgacgg
      901 gcagctcgag aagctgagcg ccgtcgcggc ggaggcggtg caggacgcca tccaggccct
      961 gctcagccat accgccgtct tccagcccgg ccgggtcttc tgtctgcgct gccagagcgc
     1021 cacctgcgag cactcggcgc cggccggcag ccgcgaggtc ttcatcggct acggcggcac
     1081 cggcacgccg cgcttcgccg acttcggcca gtggctcctc aggctgcgcg acccgcgggt
     1141 ggacctgctg taccgcgagc cgccgcagct cctgaccatc gtggcgaccg gggaggagct
     1201 ggagggcgag ctgctgccca tgttccggca gcgtgaggcg gactaccgca tccacggcca
     1261 ggtggcggcc ggatggtacc aggtcccggc ccctgtcacc ggcaacgggg gcggccatcg
     1321 ccagccgctg gcggtcacct tccagatcgt gtcgtcccag ccgcgcggcc accgccgccg
     1381 cttcggcatc aacgtcctcg gcatcggccc cgggggggaa ccgctggagc acctcttcga
     1441 ccgcctgggg cagatcccct ggaccgatcc ggtgcgctgg gcgcagtcgg tcctcgccgg
     1501 catcgagagc cagctcgcgc gctccccccg gatgccgcgg gaggcgatcg acaagcgcct
     1561 ggacgggctg gtccagggtc tggcgcggcg cctcgacaag gggtggcgcg gccgggaacg
     1621 ccgtacccac cacgccgagc agcgccacag cgaaggggac cggccgaccc gcatggccct
     1681 ggccgacctg gcccgcgcca cccccgagaa cctgctcttc gacacccgca accagaccct
     1741 ggtggtggtc ggcgagcggg ggcgggccca cgtcttcagt ctggagggga agctggtcac
     1801 ttcggtgcgc tacaaccccg cctccatcga gcggcggcgc gacaagggga tctggcgccg
     1861 cgccgccgcc gctgaggtgg agaccgtgcg cctgcgcctc cagaaggccg gggatggtca
     1921 gggcgcggcg gagggggtgt ccggctcggc ggtcagcagc gggtaggggt tgagcgcctc
     1981 gccgccccca tagatgccgt aatggaggtg ggtcggggtg ccggcggcgt tgcccgagtc
     2041 gccgacgtag cccagcaccg tccccgcctc cacccagtcg ccgggctccg gcgcatccca
     2101 ttcgtcgaga tgggcatagt aatgatagcg gccgcccggc ccgaggacgc tcaccgtccg
     2161 ccctcccagc cggttccacc ccttgcgcac cacgatcccg cgggtggcgc tcgccaccgg
     2221 cgtgccgcga ggggcgaaga tgtcgatccc ctcgtgggtc cgcccgccgc tgcgcgaggc
     2281 gccccaggtg tcgcccaggt cgccgggctc caccccctcc accggcaccg gcagccagga
     2341 gggcgccggc tggcggagga gctggacgac gcgcgtgaca tggcgcaggt agggataggc
     2401 caggaacagg aggaccgcca gcaccaccag ggcggtcagc cgggcgatca cgcgccgcca
     2461 gctcacgtca tctccaccgc gagcggatgc tccggtcggc gacgccggcc ttcacctcgt
     2521 ggaccggccg gtaggagcgg gtctcgacca cctggaacgg ctcgccgaag aggtcccgga
     2581 agcggcgctc cagctcctcc agccgcgccg gctcgatccc cttccactcc tcttcgctac
     2641 gccagcggat caccaacacc agatcggtct cgtcctcgcc gagccagacc tccttgccga
     2701 ggaagcccgc ctccgcggag aggcccggcg tccagacctc ggtgtccacc tcgacgtagc
     2761 ggtcccgcgc ccccggcgcc acccgtaccc gcatccattc gacgaccatg gattgacctc
     2821 cctaccccac cgccaccgtc gccgggtcgg gcacccgcgt cggcgccagc ccggcggccg
     2881 cggcggcctc ggcgaaccag ccggcgtcgc gcagcgcgca gcggtggaaa tcgttgttga
     2941 agaaggccca gacctcggcg tcgggccccc agaggtccgc cgccagtcgc gcccgggcgg
     3001 ccagggtctc ccgggtgtag cagcccggag ggtgcgcctc gccgccgtgg aagcgaagat
     3061 atccccaatc ggccgtccgc cactccggcg tcaggaggtg cgcgccgcgg tcggcgatgc
     3121 agagggcggc gccgtgacgg gcgaggatct cccgcacctc gtcgacgaac caggactcgt
     3181 ggcgcggctc gaccgccacc cgggcctggg gacagaggag cgccagcgcg gcgtcgagcc
     3241 gctcggcgtt gcggtgcatc tggggtggga actggagcag gaccgggccg agcttcgccc
     3301 ccagctcgcg ggcgcggctc atcagccgct cgatgggctc ctccgggtcc ttgaggcgct
     3361 tcatgtgggt gaggaagcgg ctcgccttga cggcgaacag gaaatcgccc ggggtctgcc
     3421 cggcccaggc gcggaacacg ccggcctcgg gcagccggta gaaggtggcg ttggtctcca
     3481 ccgtcaggaa gcgctcggcg taccagggga gctgggcgga cttgggcacc tcggacgggt
     3541 agaatgaccc gtcccagtgc ctgtactgcc agcccgaggt cccgatgagc accggcatgc
     3601 cgttcctgat gatcctcctg ctgctcctgg gaccgccgcc cgtggcgccg gcggaggggt
     3661 cctccgccga gctgcgccgc ctcgccgcgg gcgaggtgct ggtgacgctg gcccccgtct
     3721 ccccggccgg cggcccgcgg gaggggatcg gacgcggggt gatcgacgct ccgcccgacc
     3781 gggtctaccg ggcgctcacg gactgggccc acgccgagga gtggatgccg ttcgtcgagc
     3841 gttcggacgc agcgccgcag ccggacgggt cggtggtgag cttccaggcg ctggacctcc
     3901 ccgcccccct gggggatcgc cactacaaga tccgcgcccg cgccggggtt gccagtagga
     3961 agacgcgggt ctggcgtacg cgctggtcct acgtgccggg ctcgggcaac gtcgaggacc
     4021 accggggctc ctgggagctg accgccttcg gggacggccg caccctcgcc acctgccgcc
     4081 tctacaccga tcccggcggc gccgtcgccg cctggatgat ggaccgggcc acccgcaagt
     4141 ccctgccctg gatcttcgat ggcctgcgcc aacaggtgcg ccgctcgcgc tacgacggct
     4201 gagccctgaa gcacgtcggg gccgccttcc ggcggccccg cggcctatct atcagtgatg
     4261 aagggaaggc tcactgcgga gcgggcatgt cgtcccaggt gacggcgtgc gacatgccga
     4321 cgaggatctg gcgctggccg ctgaacggcg cgcggccgtg ggcgaccatc atgttgtcga
     4381 gcatgagcag gtcccccttc tgccagggga aggcgaccgt ctcggcgttg tagcactccc
     4441 gcaggtgttc gaggacctcc ggctcgatgg gggtgccgtc gccgtagtag gtgttggtgg
     4501 gcaggtcctc ctccgcgaac tcctcctgga gcgcctcgcg gactcggggg gtgagcgtgc
     4561 tcacgttgaa aaaggtggca tggttgaacc agaccatctc gccgctctgc gggtgcgccg
     4621 tgagcgcccg ccgcaccgcc cgggtgcgca gccggtcccc ctccttccac tcgacctgga
     4681 tgccgctctt ctggcagtgc gcctccaccg ccgccttgtc gtccgtctgg aagacctcct
     4741 tccagggcag gccgaagccg tcgccgaagt tgcggacgta catccacccc ttctcggcga
     4801 acttctggcg caccgtcgga tcgatccgct ggtagacccg gcgggtgtcg gcgatggggg
     4861 tctcgccccc ttcgcccggc tggacgttgc agaagaagaa gatccgcatc ggccaggtct
     4921 tctggtacga gttctcgttg tgcaggaaga tcggctccga cggcgggtag tccgtcgagg
     4981 tgtagatctt gccggccacc gcgtgacgcg gcgacgagcg ctccttgtac tccagcaggt
     5041 cccccgagat cgcctgcacc gtgcggtcga agtcctcgac ccgggggatg tcgaagccgc
     5101 ggaaaaggat gccgccgtgc ttcgcgagcg cggcgtcgat ggtcggccgg ttggcctccg
     5161 cccaggcggc caggctgacg ccgggcaccg cgggacgg
//
LOCUS       DASPJI010000233         4827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_74087, whole genome shotgun sequence.
ACCESSION   DASPJI010000233 DASPJI010000000
VERSION     DASPJI010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74087"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /gene="recO"
                     /locus_tag="VEL74_06190"
     CDS             <1..653
                     /gene="recO"
                     /locus_tag="VEL74_06190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HYG62151.1"
                     /translation="QGARARHSRFGGQLQPLAKAQITWFDKEGRDLVRVSAVEMVRTA
                     HPIQADLEGLLLGSYLADHLLEFAQENEPSDHLFRLLDSTLDALIAGVDRDLAARYFE
                     SWVLRLAGIFPSPRACPACGGPFSESRGGVLPPHGETLVCFLCGGDYGLVATPATLDF
                     LRQIGRAGLPEVAAAPPAARTLRQVEEICAKVRRQFLQRELRSYDVMQRTLAGLTG"
     gene            699..1631
                     /gene="glyQ"
                     /locus_tag="VEL74_06195"
     CDS             699..1631
                     /gene="glyQ"
                     /locus_tag="VEL74_06195"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HYG62152.1"
                     /translation="MHTLQDMILRLSEFWARQGCVLQQPLDLEVGAGTMHPDTFLRVL
                     GPEPWKVAYVQPSRRPADGRYGENPFRLGKHLQFQVVLKPNPHDVQRLYVQSLEAIGL
                     DLTVHDLRFEEDNWEAPTLGAWGVGWQVMLDGMEITQFTYFQQAGGLELKPISAEITY
                     GLERLTMFLGLSQSIYDIQWVEGLSYGQVRHQEEYELAKYYFEIADVAFLQQQLDGME
                     REAGRCLEAGLVLPAYECALKCSHLFNVLDARGAVSVTERVGMMKRVRDLAVACAKGY
                     VESRERLGFPLLNGGAKGGNGVGIEPVAAEVTNE"
     gene            1624..3729
                     /gene="glyS"
                     /locus_tag="VEL74_06200"
     CDS             1624..3729
                     /gene="glyS"
                     /locus_tag="VEL74_06200"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HYG62153.1"
                     /translation="MSSGGEYLLEVRSEEIPARMLEPGVRELSTRLFEELMARGIGPR
                     EVETGYTPRRLVLRFAGLPEREPDREEQVMGPPVRAAYTADGAPTPALEGFAKRTGVP
                     ADQLARVKTDKGEYLAATKKIEGRPTVDVLAEIVPRTLAGLSWPKTMRWGSGEGPWVR
                     PVHGIVSVLDGRVVPFDFFGIRAGDETEGHPILSPQRFRVDSGESYHRELAARRIEVR
                     PEERRRALLGAMQSRAGELGGSLAEDPPLLDKLVAICEIPGMMEGRIDDSFLELPREV
                     LATSLRDHQSAFTVEKDGRLLPHFLTVMDRPDDPKGRARSGNEWVVAARLADARFFYG
                     EDRKLGLEHRAEQLERLAFHERLGSYAAKTRRLEALAERICDQLGSPEEKPRAVAAAR
                     LLKADLATEMVKEFTSLQGIMGGIYAREEGQPEEVWQAIYDQYMPASTEDRIPRGRAG
                     QVVGLADRLDTLVGIFGLGLVPTGSRDPFGLRRAAQGAVRIALEGGLPVDFAAIAAEA
                     ARLYGERLTRTPEQIVDDLRPFLDDRIRYVLGLAGYAYDEIEAGLVAGVSDLPDLKAR
                     VDAVHRMRDEPDFLSVVLAAKRIVNILKDAPAQELDESALVEPAEKDLAAAFRELRSE
                     IDEAAAAHDYLRGLRRIAALAPVLDRFFVEVLVMAEDPRVRQNRLALLQAILRSVSRT
                     ARLTEVVVDKAEARAKAGG"
     gene            3816..4796
                     /locus_tag="VEL74_06205"
     CDS             3816..4796
                     /locus_tag="VEL74_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015208295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S51"
                     /protein_id="HYG62154.1"
                     /translation="MRKAILAMLLGAAALAAPAQAKVTRYLTGNAADVVVSLAGPAHD
                     FGGGGTDVDAALQWIIDQVRGCTSCSTKIDVVILRSTGADGYNDYIYAMNGVDSVETL
                     VITKASDANTAAVEATVRNAEVVFFAGGDQCDYVTNFKGTRVEAAVESVYARGGGVGG
                     TSAGFAIQGDFTYDACSGSATSADALANPYARSITFTYDFFKWTHLQNTISDSHFVAR
                     DRMGRTMAFLARQIQDGRAASALGIAADEVTSVVVDRNGLATVMGSGTAYFILADHAP
                     EVCRSRTPLTYSNFKIWKVPSGGTFNLASRPTTGYYLRSVNNGVISANPY"
BASE COUNT          715 a         1681 c         1728 g          703 t
ORIGIN      
        1 cccagggggc gcgggcgcgg cacagccggt tcggcggcca gctccagccg ctcgccaagg
       61 cgcagatcac ctggttcgac aaggagggac gcgacctggt gcgggtctcg gccgtggaga
      121 tggtgcgcac cgcccacccg atccaggcgg atctggaagg gctcctcctg gggagctacc
      181 tcgccgacca cctgctggag ttcgcccagg agaacgagcc gtccgaccac ctcttccggc
      241 tcctcgactc cactctcgac gccctcatcg ccggggtgga ccgcgacctc gcggcgcgat
      301 attttgagtc ctgggtcctg cgcctggccg ggatcttccc ctcgccccgg gcctgccccg
      361 cgtgcggcgg gccgttctcg gagagccgcg gcggcgtgct gccgccgcat ggggagacgc
      421 tggtctgctt cctgtgcggc ggcgactacg gcctggtggc cacccccgcg acgctggatt
      481 tcctccggca gatcgggcgg gcgggcctgc ccgaggtggc ggcggcccct cccgcggcgc
      541 ggaccctccg ccaggtggag gagatctgcg ccaaggtccg gcgccagttc ctccagcgtg
      601 agctccgcag ctacgatgtc atgcagcgga cgctcgccgg cctcacggga taagatcccg
      661 ggccgggcgg gagggctttc cctttttcag aggaacgaat gcacacactt caggacatga
      721 tccttcgtct ttcggagttc tgggcgcgcc agggatgcgt gctccagcag ccgctcgatc
      781 tcgaggtcgg cgccggcacc atgcatcccg acacctttct gcgcgtgctc gggcccgagc
      841 cctggaaggt cgcctacgtc cagccctccc gccgcccggc cgacggccgc tacggggaga
      901 accccttccg gctcggcaag cacctgcagt tccaggtggt cctgaagccc aacccgcacg
      961 acgtgcagcg cctgtacgtg cagagcctgg aggcgatcgg cctcgacctc acggtccacg
     1021 acctgcgctt cgaggaggac aactgggagg cgccgaccct gggcgcctgg ggcgtcggct
     1081 ggcaggtcat gctcgacggc atggagatca cccagttcac ctacttccag caggcgggcg
     1141 gcctggagct caagccgatc agcgccgaga tcacctatgg cctcgagcgc ctcaccatgt
     1201 ttctggggct gtcgcagagc atctacgaca tccagtgggt cgaggggctc tcctacggcc
     1261 aggtgcgcca tcaggaggag tacgagctgg cgaagtacta cttcgagatc gccgacgtcg
     1321 cgttcctcca gcagcagctc gacggcatgg agcgggaggc cggccgctgc ctcgaggccg
     1381 ggctcgtgct gcccgcttac gagtgcgctc tcaagtgctc ccacctgttc aacgtcctcg
     1441 acgcccgcgg cgccgtctcg gtcaccgagc gggtaggaat gatgaagcgg gtgcgcgacc
     1501 tcgccgtggc ctgcgccaag ggctacgtcg agagccgcga gcgcctcggc ttcccgctgc
     1561 tcaacggcgg agccaagggg ggcaacgggg tgggtatcga gccggtggcg gcggaggtga
     1621 cgaatgagta gtggcggtga gtacctgttg gaggtccgca gcgaggagat cccggcgcgg
     1681 atgctggagc ccggcgtgcg ggagctgtcg acgcgcctgt tcgaggagct gatggcccgc
     1741 gggatcggtc cgcgggaggt cgagaccggc tacacgccgc gccgcctggt gctgcgcttc
     1801 gccggcctgc ccgagcgcga gccggaccgc gaggagcagg tgatggggcc gccggtgcgc
     1861 gccgcctaca ccgccgacgg cgcgcccacg cccgccttgg agggcttcgc caagcgcacc
     1921 ggcgtccccg ccgaccagct cgcgcgggtg aagaccgaca agggcgagta tctcgcggcg
     1981 accaagaaga tcgagggccg gcccacggtc gacgtgctgg ccgagatcgt cccgcgcacg
     2041 ctcgccggcc tctcgtggcc caagaccatg cggtggggga gcggcgaggg cccgtgggtg
     2101 cggccggtgc acggcatcgt ttcggtgctc gacggccggg tggtcccctt cgacttcttc
     2161 ggcatccggg caggggacga gaccgagggg catcccatcc tctcgcccca gcgcttccgg
     2221 gtggacagcg gcgagagcta tcaccgggag ctggcggcgc gccggatcga ggtgcggccc
     2281 gaggagcggc gccgcgccct gctcggcgcc atgcagtccc gtgccgggga gctgggcggc
     2341 agcctcgccg aggacccgcc gctcctcgac aagctggtgg ccatctgcga gatccccggc
     2401 atgatggagg ggcgcatcga cgactccttt ctggagctgc cgcgcgaggt cctggccacc
     2461 agcctccgtg atcaccagag cgccttcacg gtggaaaagg acggccgcct gctgccgcac
     2521 ttcctgaccg tgatggaccg gccggacgac cccaaggggc gggcgcgctc gggcaacgag
     2581 tgggtggtgg cggcccggct ggccgacgcc cgcttcttct atggcgagga ccgcaagctg
     2641 gggctggagc accgggccga gcagctcgag cgcctcgcgt tccacgagcg gttggggagc
     2701 tacgcggcca agacccggcg cctcgaggcg ctggccgagc ggatctgcga tcagctcggc
     2761 tcgccggagg agaaacccag ggccgtcgcg gcggcgcggc tgctcaaggc ggacctggcg
     2821 accgagatgg tcaaggagtt cacctcgctc caggggatca tgggcggcat ctatgcgcgg
     2881 gaggaggggc agcccgagga ggtctggcag gcgatctacg atcagtacat gcccgcctcc
     2941 acggaggacc ggatcccccg cgggcgcgcc ggccaggtgg tcggcctcgc cgaccgcctc
     3001 gacaccctgg tgggcatctt cggcctgggg ctcgtgccca cgggcagccg cgacccgttc
     3061 ggcctccgcc gggcggcgca gggggcggtg cgcatcgccc tcgaaggcgg tctgccggtg
     3121 gacttcgcgg cgatcgcagc cgaggcggcg cggctctacg gcgagcggct gacccgcacc
     3181 ccggagcaga tcgtcgacga cctgcggccg ttcctggacg accgcatccg ctacgtcctg
     3241 gggctcgccg gctacgccta cgatgagatc gaggccggtc tcgtggccgg agtgagcgat
     3301 ctgccggacc tgaaggcccg ggtggacgcc gtccaccgca tgcgcgacga gccggacttc
     3361 ctgtcggtgg tgctggcggc caagcgcatc gtcaacatcc tcaaggacgc gccggcccag
     3421 gagctggacg agtcggcgct ggtggaaccg gcggagaagg atctggccgc cgccttccgc
     3481 gagctgcggt ccgagatcga cgaagccgcg gcggcccacg actacctgcg cggcctgcgc
     3541 cggatcgccg cgctcgcccc ggttctggac cgcttcttcg tcgaggtgct ggtcatggcc
     3601 gaggacccgc gggtacgcca gaaccgcctc gcgctgctcc aggcgatcct ccggagcgtc
     3661 tcccgcaccg cccgcctgac cgaggtcgtg gtcgacaagg ccgaggcgcg ggccaaggcg
     3721 ggcggatagg gcctcccgga cccgatttcc cctccttcga caccggaaga gataggcggg
     3781 cgcgttctgc gcccgcctat ccaaggaggg attcgatgcg caaagcgatt ctggcgatgc
     3841 tcctgggggc ggccgcgctg gcggcaccgg cccaagccaa ggtcacccgg tacctgacgg
     3901 gcaacgcggc cgacgtggtg gtctccctcg ccggcccggc ccacgacttc ggcggtggcg
     3961 gcacggacgt ggatgccgcc ctccagtgga tcatcgacca ggtccgcggc tgcacgagct
     4021 gctccaccaa gatcgacgtc gtcatcctgc gctcgaccgg cgccgacggc tacaacgact
     4081 acatctatgc catgaacggc gtggactcgg tggagaccct ggtcatcacc aaagccagcg
     4141 acgccaacac cgcggccgtg gaggccaccg tccgcaacgc agaggtggtc ttcttcgccg
     4201 gcggcgacca gtgcgactac gtgacgaatt tcaaggggac gcgggtggag gccgcggtgg
     4261 agtcggtcta cgccaggggc ggcggcgtcg gcggcaccag cgccggcttc gcgatccagg
     4321 gcgacttcac ctatgacgcc tgctcgggga gcgccacctc ggcggacgcg ttggcgaatc
     4381 cctacgcccg ttcgatcacc ttcacctacg acttcttcaa gtggacccac ctccagaaca
     4441 ccatctcgga cagccacttc gtggcgcgcg accggatggg ccggacgatg gcttttctgg
     4501 cgcgccagat ccaggacggc agggcggcga gcgcgctggg catcgcggcg gacgaggtga
     4561 catcggtggt ggtggaccgc aacggcctgg cgacggtgat ggggagcggc acggcctact
     4621 ttatcctggc cgatcatgcg ccggaggtct gccgctccag gacgccgctg acgtactcca
     4681 atttcaagat ctggaaggtc ccgagcggcg gcaccttcaa tctggcgagc cgcccgacca
     4741 cgggctacta cctgcgcagc gtcaacaacg gggtgatcag cgcgaacccg tactgagcct
     4801 caggctcaag ctccgacagt cgcttcg
//
LOCUS       DASPJI010000234         8570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_13742, whole genome shotgun sequence.
ACCESSION   DASPJI010000234 DASPJI010000000
VERSION     DASPJI010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8570)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8570
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13742"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="VEL74_06210"
     CDS             complement(<1..1178)
                     /locus_tag="VEL74_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYG62155.1"
                     /translation="MSSTDDRRAKLFERVAMLQERLAQPASPMERQFVEALNGLQMAL
                     QELETAEEELAQQNEELLFIRERLEMERHRYRDLFELAPDGYLVTDLAGTIEEANQAA
                     VALLGTPQFALSGKPFSVFILPAERARFRSLIRGVGVGQVLRDLELTLRRAGAEPVPV
                     LLTIVKDEPKVGHPARLRWMLRDLSARKAAEARLRESEERLRHSQRLESIGRLAGGIA
                     HSFNNLLAAIGFHSELLLDRVEGSERVHVEEIQKAGERAASLAGQLLAFGRRQVLQPR
                     VLRLDDILRQMEPMLQRLLGEDVEVRLDLRLGAGAIEADLGQLEQVILNLAVNARDAM
                     PFGGTLTLSTAGLHLAEEGYGVAQLPAGDYVQLTVADTGTGMDETTRTRIFEPFFTTK
                     "
     gene            complement(1165..3069)
                     /locus_tag="VEL74_06215"
     CDS             complement(1165..3069)
                     /locus_tag="VEL74_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015131243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HYG62156.1"
                     /translation="MKPAPAPTTEFTRLIDYLKQSRGFDFSAYKISTLMRRIQKRMRD
                     VGVQTYPDYIDYLEVHPDEFQPLFDTVLINVTEFFRDAPSWKFLADEIVPRILAEKGP
                     DRPIRAWSAGCASGEEAYTLAIVLAEALGEEAFRQRVKIYATDADEDALATARQGCFE
                     AHKLANVPPELAAKYFEPSGSRQAFRGELRRGLIFGRHDLIQDASISRLDLLLCRNTL
                     MYFNAEAQERILARFHFALSSTGYLFLGKAETLLTHSNSFRPVELRHRIFARTPSSNL
                     RDRLLALSGGSNSSDGQADLRHLRVREAAFDAGPAAQIAVDRRGFLVLANERARRLFG
                     LTESDIGRLLQDLELSYRPVELRSHIEQAYAGRGVVQLPGVEWRGGPGEASRQFEVLV
                     LPLLDAKGGLLGASVSFTDLTQAHQLRAELEKTHQELETAYEELQSANEELETTNEEL
                     QSTVEELETTNEELQSANEELETMNEELQSTNDELRVINDQVEQRSEDLDRVNTYFRS
                     VLASVRTAVVVLDQDLVVQVWSQRLRDLWGLTEDEVVGCPFLDLDIGLPVAPLAAPLR
                     DCLAGRDGTDEVVLDAVNRRGRPVQCRVRCTPLQGDRQAEGVILLIEELEMHSMPGGA
                     PEGLRDGELH"
     gene            complement(3066..3692)
                     /locus_tag="VEL74_06220"
     CDS             complement(3066..3692)
                     /locus_tag="VEL74_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015199862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HYG62157.1"
                     /translation="MLTMHKTPQVPGSTERIVPRVVALAASAGGIQALSHVLAALPPG
                     FPAAVVVLQHLSEQSPHVMADIFARRSVLPVAEARAGDVLTPGRVFVAPPGRHLLVEP
                     EGILALSDALPVHFVRPAADLLFASLAESLGTRAIAVVLSGSGLDGADGVKVIKGHGG
                     RVLVQDRATSEVFGMPRAAADTGCADRVLPLQEIAPVLIDLVGQKEEA"
     gene            complement(3839..4144)
                     /locus_tag="VEL74_06225"
     CDS             complement(3839..4144)
                     /locus_tag="VEL74_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62158.1"
                     /translation="MNVTNLNTYRSYQNLLDTGLSLDAAGEHREAFATLTEAMEKMGA
                     QADRMSLPDATALFLNLALVAGRMRRPELGMELLERSRELFARVLGPQRVVGPPVSA"
     gene            4782..5192
                     /locus_tag="VEL74_06230"
     CDS             4782..5192
                     /locus_tag="VEL74_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62159.1"
                     /translation="MTPLSDHELRSAFEAGTLKGEQFRHADHVRMAWMYLRDLPLVVV
                     LDRFPAGIRRLAQALNQPTLYHETITWAYVLLIHERIERSGAGNSWADFAAHHPDLLT
                     WKPSVLDAYYSPETLASDLAKRVFLLPDRGIRTF"
     gene            complement(5394..5927)
                     /locus_tag="VEL74_06235"
     CDS             complement(5394..5927)
                     /locus_tag="VEL74_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62160.1"
                     /translation="MRSSVQGNTPSVPCNPPNAFTASFLDRMAERDEPPTAGDADVAG
                     PWRIESLPSGGFGLFRMGESLARGFAPVSVFPSRWLALLGAAILPGTGRDPLLLLSRE
                     PDAGGAYPLTLAGGGDGDGPAGLLRLFDEKMVDGMNVLIHVLRSPESLSYVMEAAGGV
                     ALERSGAILDERTASGA"
     gene            complement(6697..8403)
                     /gene="pruA"
                     /locus_tag="VEL74_06240"
     CDS             complement(6697..8403)
                     /gene="pruA"
                     /locus_tag="VEL74_06240"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HYG62161.1"
                     /translation="MSNAIFPVTTPVNEPVRSYAPGSPETKSLQKRLQDMLSQEIEIP
                     LIIGGKEVRTGDTAKAVCPHDHGHVLAHYHQAGGKEVAMAAEAARKAWHEWSETPWEQ
                     RASVLLRAADLLAGPWRDIANGATMLNQSKTVFQAEIDSACELIDFYRFNPYFMRKLY
                     EDQPWSAPGTWNRVDYRPLEGFVFAVTPFNFTSIAGNLPTSPALMGNVVLWKPASSSV
                     YSGYWLMKLLQAAGLPDGVINFLPGKASKVGDPVMAHEDLAGVHFTGSTPVFQNMWKT
                     LGDNIANYRTYPRVVGETGGKNFLLAHPSANTDAVATALLRGAFEYQGQKCSAASRGY
                     IPASLWPRVREKLVDDLKQVKQGAPTDFRNFMAAVIDEPSYERIMGYIEFAKSSNDAE
                     ILSGGKGDRSQGYFIEPTVVLTTNPKMKLMQEEIFGPVLTLFVYDDSELEKTLELVDT
                     TSPYGLTGAIFAEDRLEVERLSRRLRHSAGNFYINDKPTGAVVGQQPFGGGRASGTND
                     KAGSMLNLVRWISLRTIKENFVPPTDFRYPHMAEELSKGTEASAASTPADRPADLVLD
                     RP"
BASE COUNT         1338 a         2910 c         2996 g         1326 t
ORIGIN      
        1 tccttggtcg tgaagaaggg ctcgaagatg cgggtgcgcg tcgtctcgtc catgcccgtg
       61 ccggtgtccg cgacggtgag ctgcacgtag tcgccggccg ggagctgcgc cacgccatag
      121 ccctcctcgg cgaggtggag cccggcggtg gacagggtga gcgtgccgcc gaacggcatg
      181 gcgtcgcggg cgttgaccgc caggttgagg atcacctgct cgagctgccc caggtcggcc
      241 tcgatggcgc cggcgcccag ccgcaggtcc agccggacct ccacgtcctc tcccagcagg
      301 cgctggagca tgggctccat ctgccgcagg atgtcgtcga gccgcagcac ccgcggctgc
      361 agcacctgcc ggcggccgaa ggcgaggagc tgccccgcca ggctcgccgc gcgctccccc
      421 gccttctgga tctcctcgac gtgcacccgc tcgctccctt cgaccctgtc gaggagcagc
      481 tcgctgtgga agccgatcgc ggccagcagg ttgttgaacg agtgggcgat gccgccggcg
      541 aggcggccga tggactccag ccgctgggag tggcggagcc gctcctcgct ctcgcgcagc
      601 cgcgcctccg ccgccttgcg ggcggagagg tcgcgcagca tccagcgcag ccgggcggga
      661 tggccgacct tcggctcgtc cttgacgatg gtgagaagca ccgggaccgg ctccgctccc
      721 gcgcgccgga gcgtcagctc caggtcgcgc agcacctggc ccacgccgac cccgcggatc
      781 agcgaccgga agcgcgccct ctcggccggc aggatgaaga ccgaaaaagg cttgccgctc
      841 agggcgaact ggggggtccc gagcagggcc accgcggcct ggttggcctc ctcgatggtg
      901 ccggcgaggt cggtcaccag gtagccgtcg ggcgccagct cgaagaggtc gcggtagcgg
      961 tggcgctcca tctccagccg ctcacggatg aagagcagct cctcgttctg ctgcgccagc
     1021 tcctcctcgg ccgtctccag ctcctgaagc gccatctgca gcccgttcag agcttcgacg
     1081 aactggcgct ccatcgggga ggccggctgt gccagccgct cctggagcat cgccacgcgc
     1141 tcgaagagct tcgcccgccg gtcgtcagtg gagctcaccg tcacgcagcc cctccggcgc
     1201 accgcccggc atcgaatgca tctccagctc ctcgatgagg aggatcacgc cctcggcctg
     1261 ccggtcgccc tgcaacggag tgcagcggac ccggcactgc acgggccggc cgcgccggtt
     1321 gacggcgtcg aggacgacct cgtccgtgcc atcgcgcccg gccaggcagt cgcgcagcgg
     1381 cgccgcgagc ggggccaccg gcagcccgat gtcgaggtcg aggaacgggc accccaccac
     1441 ctcgtcttcg gtcagccccc agaggtcccg caacctctgg ctccagacct gcaccaccag
     1501 atcctggtcg agcaccacga cggcggtccg gacgctggcc agcaccgacc tgaaataggt
     1561 gttgacccgg tcgaggtcct ccgaccgctg ctcgacctgg tcgttgatca cccgcagctc
     1621 gtcgttggtg gactggagct cctcgttcat cgtctccagc tcctcgttgg ccgactgcag
     1681 ctcctcattc gtcgtctcca gctcctcgac cgtcgactgc agctcctcat tggtcgtctc
     1741 cagctcctcg ttggccgact gcagctcctc gtaggcggtc tccagctcct ggtgcgtctt
     1801 ctccagctcg gcccggagct ggtgcgcctg ggtgaggtcg gtgaaggaga cgctcgcccc
     1861 cagcagcccg cccttggcgt ctagcaaagg aaggaccaac acctcgaact ggcggcttgc
     1921 ctcgccgggg ccgccgcgcc actccacccc cggcagctgg acgacgccgc gcccggcgta
     1981 ggcctgctcg atgtgggagc gcagctccac cgggcggtag gagagctcga gatcctggag
     2041 caggcgcccg atgtcgctct cggtcaggcc gaagagccgc cgcgcccgct cgttggcgag
     2101 gacgaggaag ccgcggcggt cgacggcgat ctgggccgcc ggcccggcgt cgaaggcggc
     2161 ttcgcggacg cgcaggtggc ggaggtccgc ctggccgtcc gaggagttgg agccgccgct
     2221 cagggccagg aggcggtcgc gcaggttgga cgacggcgtg cgcgcgaaga tccggtgccg
     2281 cagctccacc ggccggaagc tgttgctgtg ggtgagcagc gtctcggcct tgccgaggaa
     2341 caggtagccg gtggagctca gcgcgaagtg gaagcgggcg aggatccgct cctgggcctc
     2401 ggcgttgaag tacatcagcg tgttgcggca gaggagcagg tcgagccgcg agatcgacgc
     2461 gtcctggatc aggtcgtggc ggccgaagat caggccgcgc cgcagctcgc cgcggaaggc
     2521 ctggcggctg ccggacggct caaagtactt ggcggccagc tccggcggca cgttggccag
     2581 cttgtgggct tcgaaacacc cctgccgcgc cgtggccagc gcgtcctcgt ccgcgtcggt
     2641 ggcgtagatc ttgacgcgct gccggaaggc ctcctcgccc agggcttcgg cgaggacgat
     2701 cgccagggtg taagcctcct cgccggaggc gcagccggcg ctccaggcgc ggatcggccg
     2761 gtccggcccc ttctccgcca ggatccgggg gacgatctcg tcggcgagga acttccacga
     2821 cggagcgtcg cggaagaact cggtgacgtt gatgaggacg gtgtcgaaga gcggctggaa
     2881 ctcgtccggg tggacttcga ggtagtcgat gtagtcggga taggtctgga cgccgacgtc
     2941 gcgcatccgc ttctggatgc ggcgcatcag ggtggagatc ttgtaagcgc tgaaatcgaa
     3001 gcctcgggac tgtttcaggt agtcgatgag gcgggtgaat tcggtagtgg gcgcgggcgc
     3061 gggcttcaag cttcttcctt ctggcctacc agatcgatca ggaccggagc gatttcctgg
     3121 agtgggagga cgcggtcggc acagccggtg tcggcggcgg ccctcggcat gccgaagacc
     3181 tccgaggtgg cccggtcctg caccagcacc cggccgccgt gtcccttgat gactttgacg
     3241 ccgtcggcgc cgtcgaggcc gctgcccgac agcacgacgg cgatggcccg cgtccccagc
     3301 gactcggcga gcgaggcgaa cagcaggtcg gccgccgggc ggacgaaatg gacgggcagc
     3361 gcgtcggaga gggcgaggat cccctcgggt tccacgagga ggtgccggcc cggcggcgcg
     3421 acgaagaccc ggccgggcgt gaggacgtct ccggcccggg cctcggccac cggcaggacg
     3481 gaccggcggg cgaagatgtc cgccatcacg tgcgggctct gttccgacag gtgctgcagg
     3541 acgacgaccg cggccgggaa gcctggaggc agggccgcca gaacatggct caacgcctgg
     3601 atccctcccg ccgacgcggc gagcgccacg acgcgcggga cgatccgttc ggtgctcccc
     3661 ggcacctgcg gcgtcttgtg cattgtgagc atatagagga agtcttcagc cccccctttt
     3721 cctcggcgtt acagaccggg ggcagaagct tacaggaagg aactacaaaa gagaaatgcg
     3781 gagggacggc cggcgtgcca gccggcccta tccttctctc gatctggaag gcagccggct
     3841 acgccgagac cggcgggccg accacgcgct gggggccgag gacgcgggcg aacagctcgc
     3901 gggagcgctc gaggagctcc atgcccagct cggggcggcg catgcggccg gcgacgagag
     3961 ccaggttgag gaacagggcg gtggcgtcgg gcaggctcat ccggtcggcc tgggcgccca
     4021 tcttctccat cgcctcggtc agcgtggcga acgcctcccg gtgctccccg gcggcgtcga
     4081 gggacagccc ggtatccagc aggttctggt aggaacgata ggtgttcaga ttcgtcacgt
     4141 tcatacagct aaagctccgc ccccggaccg ggggatgccg acaacagctt gcacgccgta
     4201 gggctggggg gattgtagca gagccctccc gggagagggg gaagcggatg ggagccctga
     4261 gctgcaccga acctgggccg cagggggcgg tggtgcctcc ccccctgggc gggtgggcgg
     4321 tagggagcgg gggaggtcag gaggggggtt ctgctttcgg gaggggcccg cgaaccggcc
     4381 gccatccccc gcgagaacga gtccatctcc ctcgaaaacg aggccatccc cgcgggtatg
     4441 aggtccatac cccgggtacg gcgttcatcc ccggaggtag acctccatcc ccgtgggcac
     4501 ggagatcatc cctcggggga tgacggtcat ctcccccgag atggaaaccg taccccgggt
     4561 aggacgaata tttaccgggg taggacggac gtgccggcgg gtacggcggc catacccggg
     4621 gggagggtga tcatccatcg ccgctcaaga aaaaaccccc ctccttcacc tcccccgctc
     4681 cctgccgccc acccacccag ggggggaggc accacctccc tcgctcctcg ccgccggtgt
     4741 ctacaggtgg aaccctctcc cgcgatacga tcccgcccat catgactccg ctctccgacc
     4801 acgagctgcg gtcggccttc gaggccggca ccctgaaggg ggagcagttc cgtcacgccg
     4861 accacgtgcg catggcctgg atgtacctgc gggatctgcc gctcgtcgtc gtgctggacc
     4921 gcttccccgc cggcatccgg cgcctcgccc aggcgctgaa ccagccgacc ctctaccacg
     4981 agaccatcac ctgggcctac gtcctcctca tccacgaacg catcgagcgc tccggcgccg
     5041 gtaactcctg ggccgacttc gccgcgcacc atccggacct cctcacctgg aagccgtcgg
     5101 tcctcgacgc ctactacagc cccgagacgc tcgcctcgga cctcgccaaa cgggtgttcc
     5161 tgctgcccga ccgcgggatt cggactttct gagccgtctc gaccctgttc aaagctggcg
     5221 cgaccccagg gtcgaaccgc tcgaccctct ccaaagttgg cttgacccca gggtcgagcc
     5281 gttcgacccg cttcaaagtt ggctcgaccc tggggtcgaa ccgctcgacc ctctccaaag
     5341 ctggctcgac cctggggtcg agccagctcc ggccagggtc gcgtcgtctc cggctatgcc
     5401 ccggacgccg tgcgctcgtc gaggatggcg ccggaacgct ccagcgccac gccgccggcc
     5461 gcctccatca cataggagag cgattccgga gagcgcagga cgtggatcag cacgttcatg
     5521 ccgtccacca tcttctcgtc gaacagccgg aggagaccgg cggggccgtc gccgtctccc
     5581 ccacccgcga gggtgagggg ataggcaccg ccggcgtccg gctccctgga gagcagcagc
     5641 agcggatccc gccccgtgcc cggaaggatg gccgcgccga gcagcgccag ccagcgcgac
     5701 gggaagaccg acaccggcgc gaagccgcgc gccaggctct cccccatccg gaacaggccg
     5761 aagccgccgc ttggaagaga ttcgatccgc cacggaccgg cgacgtcggc gtcaccggcc
     5821 gtgggaggct cgtcacgctc ggccatccgg tcgaggaacg aggcagtgaa agcgttgggg
     5881 ggattgcacg gaacggacgg agtgttaccc tgcacagagc tgcgcatatg tcctcctagt
     5941 ggacgttgtg tggctaggcc tgggagagag ccgcaatctc tcttccgggc cgctgccacg
     6001 gatttaatac caagacatcc tggaccgcaa ccgcgggtcg cccttgagga cgtcctgatg
     6061 tcgagatcct agtgcgaagt tttgcggggt gtcaaggggg cggaagggga aaaagccccc
     6121 gcccgggagg ggcaagcccc tcccctacat cgaactgtaa cgtccggctt gtcttccgat
     6181 ctgctttccc gtctttccag gagtagatga gctgcctctc cacggtttgt gttatcttca
     6241 ggcaacacaa gacgaagctg gacgcgcgga ggccgccgat ggcaagagag aagacccgaa
     6301 ctgaggccgt gcctcggccc cctgaagact atctcgctgt cagatatcgg tgtgagttga
     6361 ttcaagacga ggacggcttc gtcgcctttc accccgatct tctggggtgc atcgctcagg
     6421 gcgagaccgc cgaagaggca atgcagaacc tggattcggc ccgccggctc tggatcgaat
     6481 cccgatacga ggatcatttg ccgatgccgg gaggtgggga tgcgcctgta ggcgcccaag
     6541 aagaaccccc ctcctgacct cccccgctcc ctcccgcacc cccgcccagg gggggaggca
     6601 cccccgtccg cgagactgaa ctatcgagag gacgcctccc cctctcccgg ggagggtgga
     6661 aggggaggcg ggagaggggg ccggggggtg agggccctac ggccggtcca gcaccagatc
     6721 cgccggacgg tccgccgggg tgctggcggc gctggcctcg gtgcccttgg agagttcctc
     6781 ggccatgtgc gggtaccgga aatcggtcgg gggcacgaag ttctccttga tcgtccgtag
     6841 ggagatccag cgcaccaggt tcagcatcga gccggccttg tcgttggtgc ccgaggcccg
     6901 gccgccgccg aacggctgct ggccgaccac cgcgcccgtg ggcttgtcgt tgatatagaa
     6961 gttgccggcg gagtgacgca gcctccggct cagacgctcg acctcgagcc ggtcctcggc
     7021 gaagatggcg ccggtcaggc cgtaggggct cgtggtgtcc accagctcca gggtcttctc
     7081 cagctccgag tcgtcataga cgaagagcgt cagcacgggg ccgaagatct cctcctgcat
     7141 cagcttcatc ttcgggttgg tggtgagcac caccgtcggc tcgatgaagt agccctgcga
     7201 gcggtccccc ttgccgccgg acaggatctc ggcgtcgttg gagctcttgg cgaactcgat
     7261 gtagcccatg atccgctcgt aggagggctc gtcgatgacc gccgccatga agttgcggaa
     7321 atcggtcggc gcgccctgct tcacctgctt gaggtcgtcc accagcttct cgcgcacccg
     7381 cggccagagc gaggccggga tgtagccgcg cgaggcggcc gagcacttct ggccctggta
     7441 ctcgaaggcg ccgcgcagga gggccgtggc caccgcatcg gtgttggccg acgggtgggc
     7501 gagtaggaag ttcttgccgc cggtctcccc caccacccgg gggtaggtgc ggtagttggc
     7561 gatgttgtcg cccagggtct tccacatgtt ctggaacacc ggcgtcgagc cggtgaagtg
     7621 gacgcccgcc aggtcctcgt gcgccatgac cgggtcgccg accttgctcg ccttgccggg
     7681 caggaagttg atgacgccgt cgggcaggcc ggcggcctgg agcagcttca tcagccagta
     7741 gcccgagtag accgagctgg aggccggctt ccagaggacg acgttgccca tcagcgccgg
     7801 cgaggtgggc aggttgccgg cgatcgaggt gaagttgaac ggagtgacgg cgaagacgaa
     7861 cccctcgagc ggccggtagt ccacccggtt ccaggtgccc ggggcggacc agggctggtc
     7921 ctcgtagagc ttccgcatga aataggggtt gaagcggtag aagtcgatca gctcgcaggc
     7981 ggagtcgatc tcggcctgga agacggtctt cgactggttg agcatggtgg cgccgttggc
     8041 gatgtcgcgc caggggccgg cgaggaggtc ggccgcgcgc agcagcacgg acgcccgctg
     8101 ctcccagggg gtctcggacc actcgtgcca ggccttcctg gccgcctcgg ccgccatcgc
     8161 gacctccttg ccgccggcct ggtggtaatg ggcgaggacg tgaccgtgat cgtgggggca
     8221 gacggccttg gcggtgtcgc cggtccgcac ctccttgccg ccgatgatca gcgggatctc
     8281 gatctcctgg gagagcatgt cctgcaagcg cttctgcaac gacttcgtct ccggggagcc
     8341 cggagcgtag gagcgcaccg gctcgttgac cggcgtggtg acgggaaaga tggcgttcga
     8401 catgtgggct cctcgaaggt ctggaaggcc gaggcttccg gccgcagtcg atgaatggga
     8461 gagacggccc cccgtccgcg gacggaccgg gaggtccccg tcatggtatc cggaccggca
     8521 cgggggctca accccgcagc gcggcccagg cgaagatcag gaagccggcg
//
LOCUS       DASPJI010000235         5314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_819020, whole genome shotgun sequence.
ACCESSION   DASPJI010000235 DASPJI010000000
VERSION     DASPJI010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819020"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VEL74_06245"
     CDS             complement(<1..814)
                     /locus_tag="VEL74_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HYG62162.1"
                     /translation="MSDEPRDPQAPPPPLPAATDQPAAEAKADAAVTAAGSPEAVGDV
                     QQPAAPAAEPVPASAAEAQAAAKVAALEKAKAAAAAKKPTHEEDPNKPLWEKEPRAPE
                     WEDAAGDPLAAALREELGDAIESARRFAGDLTLQVRRDAIAEVARSLKDRHRFTYLVD
                     LCGADYPKREPRFDVVYHAYSFDENRRVRLKVATDEATPVPTVCNVWRGANWPEREVF
                     DMYGVRFEGHPDMTRILLWEGFNGHPLRKDFPVEGIDTGSAIYPEYYEETAGP"
     gene            complement(811..1332)
                     /locus_tag="VEL74_06250"
     CDS             complement(811..1332)
                     /locus_tag="VEL74_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HYG62163.1"
                     /translation="MGLRNDYEANVLTTTYDKIFNWARRSAIWPMQFGLACCAIEMMA
                     VLDPRHDMSRFGAEVFRATPRQSDLMIVAGTVTEKMAPIVRRLWDQMPAPKWSIAMGA
                     CATCGGPYRTYAVTQGVDRIIPVDVYVPGCPPRPEALLWGLLHLQRKIDRMSIAGKPK
                     AAAALADEEGVRL"
     gene            complement(1323..1679)
                     /locus_tag="VEL74_06255"
     CDS             complement(1323..1679)
                     /locus_tag="VEL74_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HYG62164.1"
                     /translation="MADYLPILVFTVIAILFLAATLLAAKLLRHAGAATTNLDPYECG
                     NEPESFAHDHRFSVRYYLIAVLFVIFDVETVFLFPWAVMFDRLALFGFIEMTVFLVIL
                     LVGYVYIWNRGALRWA"
     gene            complement(1876..2988)
                     /locus_tag="VEL74_06260"
     CDS             complement(1876..2988)
                     /locus_tag="VEL74_06260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HYG62165.1"
                     /translation="MPTPADRLRSRIRSEGPIPFAAFMEEALYGEGGYYSRPGLPIGE
                     TGDYVTGSALSPLFGRATARLLHRLDKALNRTAELFEAGSGTGVHLDNVVTTLLERER
                     TGRRVRAWDRVVRPTPEGVRQVASLDAVGGGEIQGLVFSYELFDALPVHRLIGRQGGE
                     VGELWVDLDAEDEFVWREGDLADPALLDLLRDPDVPLEEGQVADLAPGWGPLYGELAR
                     RLGRGLLVTCDYGFERQRLLDARVRRHGTLACYSRQRVHRNPFALVGEQDLTAHVDFT
                     ALIRAGETAGLETVAFTRQALWLTACGLFEDLQSAGPEVRQQAMALLDGEGMGEEIRV
                     LVQAKGVELEAVLDPELLGQAIGAGAPGPPARKRRG"
     gene            3037..3282
                     /locus_tag="VEL74_06265"
     CDS             3037..3282
                     /locus_tag="VEL74_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HYG62166.1"
                     /translation="MIEEAVLDLLRSRAPLARDRAELPDDLRLGSGGLGMDSIALVEL
                     LLDCEEQFGIPRPVELLEGEPLTVGRLVAHVRAAMAR"
     gene            3279..4121
                     /locus_tag="VEL74_06270"
     CDS             3279..4121
                     /locus_tag="VEL74_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62167.1"
                     /translation="MNGAARWAFDGLESGLARWASRQPRWALGGAALLGRTRNRLSRR
                     WPSPDDVRALFPRLDPRAAARVAWSIGGLEARNRVLADCLRREGLDPLRPLVRGALPP
                     LRPPLLLGTFHAGAVHALGPALERLTGPVLALRHGPLYTPRPPVEIVTTEGDDQRRAA
                     VFQRALDHLRGGCFVVMALDVVPGAGLRVPCLGRTLELARGPFALARLTGAPIRPLVA
                     RWQGGGAEVEVGEPRAAGLQGEEIALATAAARWLEDYLRASPAETGLGLLRALLSEEV
                     SPSR"
     gene            complement(4105..>5314)
                     /locus_tag="VEL74_06275"
     CDS             complement(4105..>5314)
                     /locus_tag="VEL74_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYG62168.1"
                     /translation="EINEALAEAGLQGAVLEEVPRGTPPATAGGPDIAFLQPTSGTSG
                     EARAAIITQRNVMASLRTARELLGIGPDDVLVGWVPPWHDLGLLRFVLGPVYFGAPCH
                     LVTPAIRTLPLWLRTASDVRATIVGAPDFAYRLATRLVDPAGLDLSALRYATNGGEPV
                     RRSTIAAFEERFGVPGVIRPGYGLAEATLGVTGLLPGEPLRVDERGNVSCGRPLPGVE
                     VRVAREEGDAGDTGEILVRGPAVFAGYFDAEEATREALRDGWLHTGDMGRLDADGHLY
                     VLGRRRALLKRGGAPLAPRELEEAVEGVPGLRAAAAVGLPPAPGAATEEIVLAVEADP
                     ASEPRRLAAAVAGAVEAALGFAPDRVMVLAPRTLPRTANGKVRHQVLRDELVEGGLER
                     RGAVLFSGTG"
BASE COUNT          715 a         1943 c         1938 g          718 t
ORIGIN      
        1 cagggcccgc cgtctcctcg tagtactcgg ggtagatggc gctgccggtg tcgatcccct
       61 cgacggggaa gtccttgcgc agcggatggc cgttgaaccc ctcccagagg aggatgcggg
      121 tcatgtccgg gtgcccctcg aagcgcacgc cgtacatgtc gaagacctcg cgctcgggcc
      181 agttggcgcc gcgccagacg ttgcagacgg tgggcacggg ggtggcctcg tcggtggcga
      241 ccttgaggcg cacgcggcgg ttctcgtcga agctgtaggc gtggtagacc acgtcgaacc
      301 gcggctcgcg cttgggatag tcggcgccgc agaggtccac cagataggtg aagcgatgcc
      361 ggtccttgag ggagcgggcc acctcggcga tggcgtcgcg gcggacctgg agcgtcaggt
      421 cgccggcgaa gcgccgggcc gactcgatgg cgtcgccgag ctcctcgcgg agggccgcgg
      481 ccagcggatc gccggcggcg tcctcccact ccggcgccct gggctccttt tcccagagtg
      541 gcttgttcgg gtcctcctcg tgcgtcggct tcttcgccgc ggcggccgcc ttggccttct
      601 cgagggcggc gaccttcgcg gcggcctggg cctcggcggc cgaggcggga acgggctcgg
      661 cggccggagc ggccggctgc tggacgtcgc cgacggcctc cggggaaccg gcggcggtca
      721 ccgcggcgtc ggccttcgcc tcggcggccg gctggtccgt ggccgccggc aacgggggcg
      781 gcggggcctg ggggtccctg ggctcgtcgc tcacagccgc accccctcct catcggccag
      841 agcagcggcg gccttgggct tgcccgcgat gctcatgcgg tcgatcttgc gttgcagatg
      901 gagcagcccc cagaggaggg cctccgggcg cggcgggcag ccgggtacgt agacatccac
      961 cgggatgatg cggtcgaccc cctgggtgac cgcgtaggtg cggtacggcc cgccgcaggt
     1021 ggcgcaggcg cccatggcga tcgaccactt gggcgcgggc atctggtccc agaggcggcg
     1081 cacgatgggg gccatcttct cggtgaccgt gccggcgacg atcatcaggt cgctctgccg
     1141 gggcgtggcg cggaagactt cggcgccgaa gcgcgacatg tcgtgccgcg ggtcgaggac
     1201 ggccatcatc tcgatggcgc agcaggccag gccgaactgc atgggccaga tcgccgagcg
     1261 ccgggcccag ttgaagatct tgtcgtacgt cgtggtgagg acattcgcct cgtaatcgtt
     1321 tctcaagccc aacgcagtgc cccccggttc cagatgtaga cgtagccgac gaggagaatg
     1381 accaggaaga ccgtcatttc gatgaatccg aagagcgcca ggcggtcaaa catgaccgcc
     1441 cacgggaaca ggaagaccgt ctcgacatcg aagatgacga acaggacggc gatcaggtag
     1501 tagcggacgg agaacctgtg gtcgtgagcg aagctctcgg gctcgttgcc gcactcgtag
     1561 ggatcgaggt tggtggtggc ggcgccggcg tggcgcagca gcttggcggc gaggagggtg
     1621 gccgccagga acaggatggc gatcaccgtg aagaccagga tgggcaggta gtccgccatg
     1681 gggtgcttcc ctccgcgtta gcccttgact tccttcaact tagccgggtt cattctccct
     1741 tggccccagg gaggtgtcaa ggaacgcgga gcataccgcg agcgacggac gctatcctaa
     1801 gaaggaggct ccgcccccat gaagacccac ttcctcgccc cccgggggag accaggctca
     1861 aggccgccac cgtggtcatc ctcggcgttt tcgtgcgggc ggtcctggag cgccagcccc
     1921 gatagcctgc ccgagcagct ccgggtccag caccgcctcc agctccaccc ccttggcctg
     1981 gaccagcacc cggatctcct cccccatccc ctcgccgtcg aggagggcca tggcctgctg
     2041 ccggacctcg gggccggcgc tctggaggtc ctcgaagagg ccgcaggcgg tgagccagag
     2101 ggcttgccgg gtgaaggcga cggtctcgag acccgcggtc tcgccggcgc ggatcagcgc
     2161 ggtgaagtcc acgtgggccg tgaggtcctg ctcccccacc agggcgaacg ggttgcggtg
     2221 cacccgctgg cggctgtagc aggcgagggt cccgtgacgg cggacgcggg catcgaggag
     2281 gcgctgccgc tcgaagccgt agtcgcaggt gaccaggagg ccgcgcccca gccggcgcgc
     2341 cagctcaccg tacagcggtc cccagccggg cgccaggtcg gccacctggc cctcctccag
     2401 ggggacgtcc gggtcccgca gcaggtccag cagcgccgga tcggcgaggt ccccctcgcg
     2461 ccagacgaat tcatcctccg catcgagatc cacccagagc tcccccacct cgccaccctg
     2521 gcggccgatc agccggtgaa cgggcagggc gtcgaacagc tcataggaga agacgagccc
     2581 ctggatctcc ccgccgccga ccgcgtcgag gctggcgacc tgccgcaccc cctccggcgt
     2641 cgggcggacg acgcggtccc aggcccgtac ccgccgcccg gtccgctccc gctcgagcag
     2701 ggtggtgacc acgttgtcca ggtggacccc ggtgccggac cccgcctcga agagctccgc
     2761 cgtccggttc agcgccttgt ccagccggtg cagcaggcgc gccgtggcgc ggccgaacag
     2821 gggggacagc gccgagccgg tgacgtagtc gcccgtctcc ccgataggca gaccggggcg
     2881 gctgtagtag cccccctcgc catagagggc ctcctccatg aaggccgcga aggggatcgg
     2941 gccctcggag cggatacggc tgcggaggcg gtcggcgggg gtcggcatgg agagaagctt
     3001 agccgaggta ctcttcattg gatggaaacg cccccagtga tcgaagaagc cgtcctggac
     3061 ctcctccgct cgcgggcccc actggcccgg gaccgcgccg agctccccga tgacctgcgg
     3121 ctggggtcgg gcggcctggg gatggattcc atcgccctgg tggagctgct cctcgactgc
     3181 gaggagcagt tcgggatccc gcggccggtg gagctgctgg aaggagagcc cctcacggtg
     3241 ggccggctcg tggcccacgt gcgggccgcg atggcacggt gaacggggcg gcgcgatggg
     3301 cgttcgacgg tctggagagc gggctggccc gctgggcctc gcggcaacca cggtgggcgc
     3361 tcggcggcgc cgcgctcctc ggccggacgc gcaaccggct gtcccggcgc tggccttccc
     3421 ctgacgacgt ccgggcgctc ttcccccgtc tcgatcctcg cgcggcggcc cgcgtcgcct
     3481 ggagcatcgg cggcctcgag gcgcgcaacc gggtgctggc cgactgtctc cggcgggagg
     3541 ggctcgaccc gttacggccc ctggtgcgcg gcgccctccc accgctccgc cctcccctcc
     3601 tcctcgggac cttccacgcc ggagccgtgc acgccctcgg accggcgctg gaacgcctga
     3661 ccggcccggt cctcgcgctg cggcacggcc ctctctatac ccccaggccg ccggtggaga
     3721 tcgtcaccac ggaaggggat gaccagcgcc gggcggccgt gttccagcgc gctctcgatc
     3781 acctcagggg cggttgcttc gtggtcatgg ccctggacgt cgtgccggga gcgggcctcc
     3841 gggtcccctg cctcggccgc acgctcgagc tggcccgcgg ccccttcgcc ctggcgcggc
     3901 tcaccggcgc gccgatcaga cctctggtgg cgcgctggca ggggggcggg gcggaggtcg
     3961 aggtgggtga gccgcgcgcc gccggtctcc agggagagga gatcgccctg gccaccgccg
     4021 ccgcgcgctg gctcgaggac tacctccggg cctcgcccgc cgagacgggc ctgggcctgc
     4081 tccgcgccct cctctccgaa gaggtcagcc cgtcccgctg aacagcaccg cgccccgccg
     4141 ctccagcccg ccctccacca actcgtcgcg caggacctgg tgccgcacct tgccgttggc
     4201 ggtgcggggc agcgtccgcg gcgccagaac catcacccgg tcgggagcga agccgagggc
     4261 cgcttcgacg gcgccggcca cggccgcggc aaggcgccgg ggctcggacg ccggatcggc
     4321 ctcgacggcc aggacgatct cctcggtcgc cgcgcccggc gccggcggca gccccaccgc
     4381 ggcggcggcg cgcagccccg gtaccccttc gaccgcctcc tccagctccc gcggcgccag
     4441 cggcgcgccc ccgcgcttga ggagcgcccg gcggcggccc aggacataga gatgtccgtc
     4501 cgcgtcgagc cgccccatgt cgccggtgtg gagccagccg tcgcggagcg cctcgcgggt
     4561 cgcctcctcg gcgtcgaagt agccggcgaa gaccgccggc ccgcggacca ggatctcgcc
     4621 ggtatcgccg gcgtcaccct cctcccgggc gacgcgcacc tcgacgcccg gcagcggccg
     4681 gccgcaggag acgttgccac gctcgtccac ccgcagcggc tcgccgggga ggaggcccgt
     4741 cacccccagg gtcgcctcgg ccagcccgta gccggggcgg atcacccccg gcacgccgaa
     4801 acgctcctcg aaggcggcga tcgtgctccg ccggaccggc tcgccgccgt tggtggcata
     4861 gcggagcgcc gacaggtcga ggccggcggg gtccacgagc cgcgtcgcca gccggtacgc
     4921 gaagtcgggc gcgccgacga tggtggcccg gacatcggac gccgtccgca gccacagcgg
     4981 cagggtgcgg atggccggcg tcaccaggtg acagggggcg ccgaagtaaa ccgggccgag
     5041 gacgaaccgg agcagcccca ggtcgtgcca cggcggcacc caccccacca ggacatcatc
     5101 ggggccgatg cccagcagct cccgggccgt ccgcaacgag gccatgacgt tgcgctgggt
     5161 aatgatcgcc gcccgtgcct cgccggacgt tcccgaggtc ggctggagga acgcgatgtc
     5221 cgggcccccc gcggtcgccg gcggcgtccc ccggggtacc tcctcgagaa cggcgccttg
     5281 aagccccgcc tcggccagcg cctcattgat ctcg
//
LOCUS       DASPJI010000236         8525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_247635, whole genome shotgun sequence.
ACCESSION   DASPJI010000236 DASPJI010000000
VERSION     DASPJI010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8525)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8525
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247635"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VEL74_06280"
     CDS             complement(<1..373)
                     /locus_tag="VEL74_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HYG62169.1"
                     /translation="MYKSDPFDPAGSREAFTKHLGMFAFPEGSHLLARTAPLGNWLEQ
                     REGLGTWPYARTLEQAPGTVTRLESQGGARVEGINFGSQDYLGLSAHPALRNAAMNAL
                     LEFGPHTASSPMLQGNTLLSRQ"
     gene            complement(492..1307)
                     /locus_tag="VEL74_06285"
     CDS             complement(492..1307)
                     /locus_tag="VEL74_06285"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HYG62170.1"
                     /translation="MTSPLPLSGIRVVVTRAAHQAAELAAAFAAAGAAVEPLPLLEIC
                     PPADPRPLERAASELSLYDWLVFTSTNAVDAFLPLTGGALPPRLQVAAVGAATAEALR
                     NEGIEVHLQGSGGDSDAEGLLAELAPRVARRRRVLLPQAADARPLLVDGLAAAGADPV
                     AVVAYDKRLPSGAVERARELFGAYPLGWVTFTSPRIVRHFADLLGAGWERRRSELRAA
                     SIGRVTTAELRRQGVEPAAEAASPGDEAMVEAVTRAVASSSSLPRGGAPHPSG"
     gene            complement(1304..2263)
                     /gene="hemC"
                     /locus_tag="VEL74_06290"
     CDS             complement(1304..2263)
                     /gene="hemC"
                     /locus_tag="VEL74_06290"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HYG62171.1"
                     /translation="MISPEPPLPAARTFRLGTRRSALALAQSGMMARALEAQHPGLIV
                     EMVPIVTTGDVVTGDLSEVGGKGLFTRELEQGLLEGSLDLAVHSLKDLPVTLPDGLAV
                     AAFPERADPRDVLVSEVAADVDGLPEGAVVLTGALRRRAQLLLRRPDLQVEPIRGNVD
                     TRLRKWRETGAGAVILAGAGLARLGLRDIPAHPLSPEVMVPAPGQGILALEVRAGGPA
                     AAFVAVLNHPDAARAAEAERRVVAAFGGDCTLPLAAWARPEEVGGKGLRLTAVLLTPD
                     GRHAARGEAVGEDPVRVAAACVAAMREDGAEEVRARIRQATGR"
     gene            complement(2250..3578)
                     /gene="hemA"
                     /locus_tag="VEL74_06295"
     CDS             complement(2250..3578)
                     /gene="hemA"
                     /locus_tag="VEL74_06295"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HYG62172.1"
                     /translation="MSAPQLPGLPETSRPQEVRTDPPLLLVGTDFRCSPLEMRERVSY
                     DSAAGEELLVHLLAGAGVAEAFLLSTCNRTEVYIHPRDEDAAYRATLDLAFLRRVPEL
                     EKPGRLYVKRNGEAARHLLAVASGLESMVLGEPEILGQVKQAAALAEAVGAEGPILRR
                     LLRSAMSAGARSRQETGISAGAVSLGYATVELARQIFKGLEECRVLLLGAGETARSVA
                     RPLVERGARELKVANRGAERARQFQEEFPQTLLIPFEERVAAVRTAEVVVVSTGADEP
                     VLTHRHLQEAMAARSTDPLLVVDLGVPRNVEPSAGRIGNLFLHTVDSLEVLIQRNLKL
                     RKEEVPRVEEILDQEMGHFRAWYRGLGAEPVVARLQKQAERVRQHEVAQMLHRFPPDT
                     HADLERLTRSLVRKILHHPSVHLRNHEGSQDASRLELVRELFHLEDDDQP"
     gene            complement(3578..4399)
                     /gene="ccsA"
                     /locus_tag="VEL74_06300"
     CDS             complement(3578..4399)
                     /gene="ccsA"
                     /locus_tag="VEL74_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HYG62173.1"
                     /translation="MTTFLDAATILLPVAYLLVAIAYGFVFFAPQPLPERAATPGLRG
                     TVLFHLAYLTALTYHWKQFPAATVSQALSIVAFAVAVVYAYVEWHGRERSTGFWMVSL
                     VFFFQLLSSILTRPQPPSLEIFHNPFFAFHVSLALLGYASFVVGAAYAFLFLQLYREL
                     KAGRFSTFYGKLPPLETLERMMLGALSVGFVSLTGAVITGAVYAERLDQPWLHDPKIL
                     ITLATWVLYAAALLLRRMRRWQGRQTAMASLAGLGAILFSLMAVNFFFTDFHGFL"
     gene            4552..5883
                     /locus_tag="VEL74_06305"
     CDS             4552..5883
                     /locus_tag="VEL74_06305"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HYG62174.1"
                     /translation="MANVVIVGMQWGDEGKGKIVDLLCPAFDGVVRYQGGHNAGHTVR
                     FRDQHFSLHLIPSGILHPGMLCALGNGMVIAPDAFLTELGRLYEAGVRFDGRLFVSNR
                     AHVLLPCHAILDKAREEAKGQTRIGTTSRGIGPAYENKASRFGIRMCDLAAPDLEERL
                     RSQLGRVQLELTSLGGEALPSPAAYADQCRGWAERLKPCLRDTEQLLNGWIAEGKCLL
                     FEGAQGALLDVDHGTYPYVTSSNSTAGGACTGTGVPPTRIDGAIGVLKAYTTRVGGGP
                     FPAEIEGAAGDFLRKRGNEFGTTTGRPRRCGWLDTVVARYAQLLNGVDMIALTKLDVL
                     DDFDEIPVCVGYQIGGRTVRELPPDQRSLESAIPVLRTFKGWKRSTVGVVDEAALPQE
                     ARDYVAFIEDEIGAPVTLLSTGPRREETIVREDASLTRLTSGRLARVRENL"
     gene            5911..5986
                     /locus_tag="VEL74_06310"
     tRNA            5911..5986
                     /locus_tag="VEL74_06310"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5944..5946,aa:Lys,seq:ttt)
     gene            6014..7417
                     /locus_tag="VEL74_06315"
     CDS             6014..7417
                     /locus_tag="VEL74_06315"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYG62175.1"
                     /translation="MTTAGISESAGPASSGFRTFVLIWLGQMVSMLGSGLTSFALGVW
                     IYQRTGSATSFALLTFFVIVPAMLVLPLAGVLVDRWDRRRVMMASDSLAALGTLALAL
                     LVWSGRLETWHIYAVVVFNSAVSSLQMPAFSATVPLLVAKRHLGRTAGMMQLGAAVPT
                     ILAPLVAGALVAVIGLHGILIVDLVTFLFAVSMLFIVRLPNPPRGGVEDAAGAEQGPV
                     SRDALYGWTYLRERPGLLALLFMFAGVNFCLGFLNALLTPMILSFASEKALGTVMALA
                     GCGMLVGGVAMAVWGGPKRRMQGVFGFLLLAAVILSLSGLRENVTLIAAAAFAFLCCF
                     PLINGCAAAILQTKIAPQVLGRAMAAQQLVAMSALPVAILLAGPLADHVFEPLLAPGG
                     PLAGTVGRVIGVGPGRGIGLLFMVLGGLIVLAVAAGWANPRVRNLEDELPDAIGDEPP
                     PVSPLGPAPQEPAPGSL"
     gene            complement(7368..8282)
                     /locus_tag="VEL74_06320"
     CDS             complement(7368..8282)
                     /locus_tag="VEL74_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HYG62176.1"
                     /translation="MHTVHLVDASPYIFRAHFSLPSSIKSPTGERTGAVYGFASFLLK
                     LIADEKPTHLGVAFDRNLNGSFRNDYYPAYKAQREAPPPEIVAQIDPCLEVAAALGAT
                     TWIDDRYEADDLIATVCEAVTQRGHQVVVVTSDKDLAQLVSDRVTLLDFAKEARFTPD
                     DVREKFGVRPEQITDLLALAGDPVDNIPGVPGIGRKTAAELLAAFGSVEDLYARLEEL
                     RVSRIRGARSLYTKLTDARESAFLSKRLATVAHDAPLDPPAEKLAADGLRALAYRGAD
                     TGRVGELFDRLGFKGIRERVPAAPGQEG"
     gene            complement(8309..>8525)
                     /locus_tag="VEL74_06325"
     CDS             complement(8309..>8525)
                     /locus_tag="VEL74_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62177.1"
                     /translation="SGLEKEFARRVETRYVVAETPESRELIAELGFRSHGLVIRSLEG
                     EVLWKQADHEVNVEDARQALRELLARH"
BASE COUNT         1225 a         2852 c         3207 g         1241 t
ORIGIN      
        1 gctggcgcga cagcagggtg ttgccctgga gcatgggcga cgaggccgtg tgggggccga
       61 attccagcag cgcgttcatg gcggcgttgc gcagggcggg atgggcggag aggccgagat
      121 agtcctggga gccgaagttg atgccctcca cccgggcgcc tccctggctc tcgagccggg
      181 tgacggtgcc gggagcctgc tcgagggtcc gggcataggg ccaggtcccg aggccctcgc
      241 gctgctccag ccagttcccc aggggagcgg tcctcgcaag gagatgggaa ccttcgggaa
      301 aggcgaacat ccccaggtgc ttggtgaacg cctcgcggct accggcagga tcgaacggat
      361 cggatttgta catgggaatc ctcggaaggt gggtggaaga gaagtgtaac cggaggctgc
      421 cggcaatccc cgccggcccc tacttcggcg tcggcgagct accggtcagg gttgggaggc
      481 gggatgcggg cctaccccga agggtgggga gcgccgcccc gtggaagcga gctggagctc
      541 gccaccgctc gcgtcaccgc ttccaccatg gcttcgtctc ccggcgaagc ggcctcggcg
      601 gcgggctcga cgccctggcg gcggagctcg gcggtggtga cgcggccgat ggaggcggca
      661 cgcagctcgg agcggcggcg ttcccagccg gctccaagga gatcggcgaa gtggcggacg
      721 atgcgggggc tggtgaaggt cacccatccc agagggtagg ctccgaacag ctcgcgcgcc
      781 cgctccacgg caccggacgg caggcgcttg tcgtaggcga ccacggccac gggatcggcg
      841 ccggctgcgg ccaggccatc caccagcagg ggacgggcgt cggcggcctg ggggaggagg
      901 acgcggcgcc gtctcgccac ccgcggcgcc agctcggcaa ggaggccctc ggcgtccgag
      961 tcgccgccgg agccctgcaa gtgcacctcg atcccttcgt tcctcagcgc ttcggcggtg
     1021 gcggcaccca cggcggccac ctggaggcgg gggggcaggg cgccgccggt cagggggagg
     1081 aacgcgtcca cggcgttggt ggaggtgaag accagccagt cgtagagcga cagctcggag
     1141 gcggcgcgct ccaggggccg ggggtcggcc ggagggcaga tctccagcag ggggagcggc
     1201 tccacggcgg cgccggcggc ggcgaaggcg gcggccagct ccgcggcctg gtgggcggcg
     1261 cgggtgacta cgacgcggat tccagacagg gggaggggag aggtcatcgg cccgtggcct
     1321 ggcggatgcg ggcgcggacc tcctcggcgc cgtcctcccg catggcggcc acgcaggccg
     1381 ccgccacccg gaccgggtcc tcgcccacgg cctcgcctct cgcggcgtga cggccgtcag
     1441 gggtcaggag cacggcggtc aggcgcaggc ctttccctcc cacctcctcg ggccgggccc
     1501 aggcggcgag ggggagcgtg cagtcgccgc cgaaggcggc caccacccgg cgctcggcct
     1561 cggcggcgcg ggcggcgtct ggatggttca gtacggcgac gaacgccgcg gccgggccgc
     1621 cggcccggac ctccagggcg aggatgccct gcccgggagc ggggaccatc acctcggggg
     1681 acagcggatg cgccgggatg tcgcgcagcc ccagccgggc gaggccggcg ccggccagga
     1741 tcacggcccc ggcgcccgtc tcgcgccact tgcgcagccg ggtgtcgacg ttgccgcgga
     1801 tcggctccac ctggagatcg gggcggcgga ggaggagctg ggcgcggcgg cggagggctc
     1861 cggtgagcac caccgcgccc tcgggcaggc cgtccacgtc cgccgccacc tcgctcacca
     1921 ggacgtcgcg cgggtcggcc cgctccggaa aggcggcgac cgccaagccg tccgggaggg
     1981 tgaccgggag gtccttgagg ctgtggacgg cgaggtcgag ggagccttcg aggaggccct
     2041 gctccagctc gcgggtgaag aggcccttgc ccccgacctc ggacaggtcg ccggtgacca
     2101 cgtcgccggt ggtgacgatg gggaccatct cgacaatgag gccggggtgc tgcgcctcca
     2161 gggcacgggc catcatcccg gactgcgcca gggcgagggc gctccggcgg gtccccagac
     2221 ggaaagtacg ggctgccggg agggggggct cagggctgat catcgtcttc caggtgaaac
     2281 agctcgcgca ccagctcgag gcgggaggcg tcctgactcc cctcgtggtt gcgcaggtgg
     2341 acgctcgggt ggtgcaggat cttgcggacc agcgaccggg tcaggcgctc gaggtcggca
     2401 tgggtgtccg gcgggaagcg gtggagcatc tgcgccacct cgtgctggcg cacgcgctcg
     2461 gcctgcttct ggaggcgcgc caccaccggc tcggcgccga ggccgcggta ccaggcccgg
     2521 aagtggccca tctcctggtc gaggatctcc tcgaccctgg ggacctcctc cttgcggagc
     2581 ttcaggttgc gctggatcag gacctccagc gagtcgacgg tgtggaggaa caggttgccg
     2641 atccggccgg cggagggctc gacgttgcgc ggcacgccga ggtccactac cagcagcgga
     2701 tcggtcgacc gggcggccat ggcctcctgg aggtggcggt gggtgaggac cggctcgtcg
     2761 gcgccggtcg agaccaccac cacctcggcg gtgcgcaccg cggccacgcg ttcctcgaag
     2821 gggatgagga gggtctgagg gaactcctcc tggaactggc gggcgcgctc ggcgccgcgg
     2881 ttggccacct tgagctcgcg ggcgccgcgc tcgaccagcg ggcgcgcgac ggagcgggcg
     2941 gtctcgccgg cgccgaggag gaggacccgg cactcctcga gccctttgaa gatctggcgc
     3001 gccagctcga cggtggcgta gcccagggag acggcgccgg cggagatccc ggtctcctgg
     3061 cgggagcgcg cgccggccga catggcgctg cgcagcaggc ggcgcaggat cggcccctcg
     3121 gcccccaccg cttcggccag ggcggcggcc tgtttgacct ggcccaggat ctcgggctcg
     3181 cccaggacca tggactccag gccgctcgcc acggcgagca gatggcgggc cgcctcgcca
     3241 ttgcgcttga cgtagagccg ccccggcttc tccagctccg gcacgcggcg caggaaggcc
     3301 agatcgaggg tggcccggta agcggcgtcc tcgtcgcgcg gatggatgta gacctcggtg
     3361 cggttgcagg tcgagagcag gaacgcctcg gcgaccccgg cgccggccag cagatggacc
     3421 agcagctcct cgccggcggc ggagtcgtag gagacccgct cccgcatctc cagcggcgaa
     3481 cagcggaaat cggtgccgac gaggaggagc ggcggatccg tcctgacctc ctgcgggcgc
     3541 gaggtctccg gcaggccggg gagctggggc gcagacatca gaggaacccg tggaagtcgg
     3601 tgaagaagaa attgaccgcc atcagggaga acaggatggc gcccaggccg gcgaggctgg
     3661 ccatggcggt ctgccggccc tgccagcgcc gcatccggcg cagcagcagg gccgcggcat
     3721 agagcaccca ggtggccagg gtgatcagga tcttgggatc gtgcagccag ggctggtcga
     3781 gccgctcggc atagacggcg ccggtgatca ccgcgccggt caggctgacg aagcccaccg
     3841 acagcgcgcc gagcatcatc cgctccagcg tctccagggg cggcagcttg ccatagaagg
     3901 tggagaagcg gcccgccttg agctcgcggt agagctgcag aaagaggaaa gcgtaggcgg
     3961 cgccgaccac gaaggaggcg tagcccagga gggccagcga cacgtggaag gcgaagaacg
     4021 ggttgtggaa gatctccagg ctcggcggct ggggccgggt gaggatggag gacagaagct
     4081 ggaagaagaa gaccagcgac accatccaga agccggtgga gcgctcccgt ccgtgccact
     4141 cgacgtacgc gtagacgacc gccacggcga aggccacgat ggagagggcc tgggataccg
     4201 tcgctgccgg gaactgcttc cagtggtagg tgagggcggt caggtaggcc aggtggaaca
     4261 gcaccgtgcc gcgcagcccg ggggtggccg cccgctccgg cagcggctgc ggagcgaaga
     4321 agacgaaccc gtacgcgatg gccaccaaaa gataggcgac cggcaggagg atggtggcgg
     4381 cgtccaggaa cgtggtcaag cggattcctc ggcaggcgga gtaaggcagt cgcctcgatg
     4441 ctagcacctt cttgcccggc cctacatcct ctgctagtgt ccctcgggtc ccgcaagtcg
     4501 cgggcccaca gggacatcga cctttttgcc tggacaacag ccggagccgc catggccaac
     4561 gtcgtgatcg tagggatgca gtggggtgac gaggggaagg ggaagatcgt ggatctcctg
     4621 tgcccggcgt tcgatggcgt ggtgcgatac cagggggggc acaacgcggg gcacacggtc
     4681 cgtttccggg accagcactt ctccctgcac ctgatcccct ccggcatcct ccatcccggg
     4741 atgctctgcg ccctcggcaa tggcatggtg atcgcccccg atgccttcct gaccgagctg
     4801 ggccggctct acgaggccgg ggtccggttc gacggccggc tgttcgtctc caaccgggcg
     4861 cacgtgctgc tgccctgcca cgccatcctc gacaaggcgc gggaggaggc gaagggtcag
     4921 acacggatcg gcacgacctc gcgggggatc gggccggcct acgagaacaa ggcgtcgcgc
     4981 ttcggcatcc ggatgtgcga cctggccgcc cccgacctgg aggagcggct gcgcagccag
     5041 ctcggtcgtg tccagctcga gctgaccagc ctgggcggcg aggcgctgcc gtcgcccgcc
     5101 gcctacgccg accagtgccg cggctgggcg gagcgcttga agccttgcct gcgtgacacc
     5161 gagcagctcc tcaacggctg gatcgccgag ggcaagtgcc tgctgttcga aggggcccag
     5221 ggggcgctgc tggacgtgga ccacggcacc tatccctacg tcaccagctc caactccacg
     5281 gcgggagggg cctgcacggg caccggcgtg ccgccgacgc ggatcgatgg cgccatcggc
     5341 gtgctcaagg cctacaccac tcgggtgggg ggcgggccgt tcccggcgga gatcgagggg
     5401 gcggccggcg acttcctgcg caagcgcggc aacgagttcg gcaccaccac gggccggccg
     5461 cgccgctgcg gctggctcga cacggtggtg gcccgctatg cccagctcct gaacggcgtc
     5521 gacatgatcg ccctgaccaa gctggacgtc ctcgacgact tcgacgagat cccggtctgc
     5581 gtcggctacc agatcggcgg ccggacggtg cgggagctgc cgccggacca gcggagcctc
     5641 gagtccgcca tccccgtgct gcgcaccttc aagggctgga agcgctcgac ggtaggtgtc
     5701 gtggacgagg ccgcccttcc ccaggaggcg cgggactacg tcgccttcat cgaggacgag
     5761 atcggcgcgc cggtgacgct gctctccacc ggcccgcggc gggaggagac catcgtccgc
     5821 gaggacgcga gccttacccg cctcacctcc ggccggctcg ccagggtgcg cgagaacctc
     5881 tagagggttt cggctatact cgcctaccgg gggccgttag ctcagttggt agagcagcgg
     5941 acttttaatc cgcgggtcca gagttcgagt ctctgacggc ccaccagtcc gcgaccgcac
     6001 atccattccg ttcatgacca ccgcaggtat ctccgagtct gccggtccgg cctcatccgg
     6061 cttccggacg ttcgttctca tctggctcgg gcagatggtg tcgatgctcg ggtccgggct
     6121 cacctccttc gcgctggggg tgtggatcta ccagcgcacg gggtcggcca ccagcttcgc
     6181 gctgctgacc ttcttcgtca tcgtgccggc gatgctcgtc ctgccgctgg caggggtgct
     6241 ggtggaccgc tgggaccggc ggcgggtgat gatggccagc gactcgctgg cggccctggg
     6301 caccctggcc ctggcgctgc tggtgtggag cggccggctc gagacctggc acatctatgc
     6361 cgtggtggtc ttcaactcgg cggtgagctc cctgcagatg ccggcgttct ccgccaccgt
     6421 cccgctgctg gtggcgaagc gccacctggg ccggaccgcc ggcatgatgc aactcggcgc
     6481 cgccgtgccg accatcctgg cgccgctggt ggcgggggcc ctggtcgccg tcatcgggct
     6541 ccacggcatc ctgatcgtcg acctggtcac cttcctcttc gccgtctcga tgctcttcat
     6601 cgtccggttg ccgaacccac cgcgtggcgg agtcgaggac gccgcggggg cggagcaggg
     6661 gccggtctcg cgagatgccc tttacggctg gacctacctg agggagcggc caggcctgct
     6721 ggcgctgctc ttcatgttcg ccggcgtgaa cttctgtctg ggcttcctga acgcgctgct
     6781 cacgccgatg atcctgagct tcgcctcgga gaaggcgctc ggcaccgtca tggcgctcgc
     6841 cggctgcggc atgttggtgg ggggcgtcgc catggccgtc tggggagggc cgaagcggcg
     6901 catgcagggt gtcttcggct tcctgctgct cgccgccgtg atcctctcct tgagcggcct
     6961 gcgggagaac gtgacgctca tcgcggcggc cgcgttcgcc ttcctctgct gcttcccgct
     7021 gatcaacgga tgtgccgcgg ccatcctgca gacgaagatc gcgccgcagg tgctggggcg
     7081 ggccatggcg gcgcagcagc tcgtcgctat gtcggcgctg ccggtcgcca tcctcctggc
     7141 cgggcctctg gccgaccatg tcttcgagcc gctgctggct ccgggtggac cgctggccgg
     7201 cacggtggga cgggtgatcg gggtcgggcc gggccgcggc atcggcctgc tcttcatggt
     7261 gctcggcggg ctgatcgtcc tcgctgtggc cgcggggtgg gccaacccgc gggtgcgcaa
     7321 cctcgaagac gagctgcccg acgcgattgg cgacgagcct ccgcccgtca gccctcttgg
     7381 cccggcgccg caggaacccg ctcccggatc cctttgaagc ccagccggtc gaagagctcg
     7441 ccgactcgcc cggtgtccgc tccccggtag gccagggctc gcagcccgtc cgcggccagc
     7501 ttttcggccg ggggatcgag cggagcgtcg tgcgccaccg tggcgagccg cttggagagg
     7561 aacgccgact cgcgggcgtc ggtgagcttg gtgtagaggc tccgcgcgcc ccggatgcgt
     7621 gagacgcgga gctcctccag gcgggcgtag agatcctcca cggagccgaa ggcggcgagc
     7681 agctccgcgg ccgtcttgcg gccgatgccg ggcacgcctg ggatgttgtc caccggatcg
     7741 ccggcgaggg cgaggaggtc ggtgatctgc tcgggccgga ccccgaactt ttcccgcacg
     7801 tcgtccggcg tgaagcgagc ctccttggca aagtccagca aggtcacccg gtcgctcacg
     7861 agctgcgcga ggtccttgtc ggaggtcacc accaccacct ggtgtcccct ctgggtgacg
     7921 gcttcacaga cggtggcgat caggtcgtcg gcttcgtagc gatcgtcgat ccaggtggtg
     7981 gctccgagcg ccgcggccac ctccaggcag ggatcgatct gggccacaat ctcgggcggc
     8041 ggggcctcgc gctgtgcctt gtaggcgggg tagtagtcgt tgcggaagct gccgttcagg
     8101 ttgcggtcga aggccacccc gagatgggtg ggcttctcgt ctgcgatgag cttcaggagg
     8161 aagctggcga agccgtagac cgcgccggta cgctcgcccg tcggactctt gatggaggac
     8221 ggcagcgaga agtgggcgcg gaagatgtaa gggctggcgt cgacgagatg gacggtgtgc
     8281 aagacggctt gttctcctcg ggctctcgtc agtgccgggc gagcaactcg cgaagggcct
     8341 gccgggcgtc ttcgacattg acctcgtggt ccgcctgctt ccaaaggacc tcgccttcca
     8401 gggagcggat gaccagacca tggctgcgga accccaactc cgcgatgagc tcgcgggact
     8461 cgggcgtctc ggccacgacg tagcgggtct cgacccgccg cgcaaactct ttttcgagcc
     8521 cgctc
//
LOCUS       DASPJI010000237         4666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_188225, whole genome shotgun sequence.
ACCESSION   DASPJI010000237 DASPJI010000000
VERSION     DASPJI010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188225"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4021
                     /locus_tag="VEL74_06330"
     CDS             <1..4021
                     /locus_tag="VEL74_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62178.1"
                     /translation="LAILKTGAAYVPLDPTYPAERLAYMLGDSGAPLLLARRAWPGGE
                     PVAGVTLVDLDGLELEGADEEDLEIEIPSGSPAYVIYTSGSTGRPKGVLVTHANVLRL
                     FASTRELFGFGSTDVWTLFHSYAFDFSVWEIWGALLHGGRLVVVPFAVSRSPEKLHAL
                     LRQEEVTVLNQTPSAFQQLVWAESEIRQREGSAPRLPRLRWVIFGGEALDLARLEPWI
                     EHHGDRRPELVNMYGITETTVHVTQRRIRREDLSAGGSLIGRPLPDLSVYLLNRGGGL
                     VPIGVPGEIWVGGAGLGTGYLGRPGLTAERFVPDPFGAEAGGRLYRSGDLARHLANRE
                     LQYLGRIDHQVKVRGFRIELGEIENVLRRHPGVRDVAVLVRPGALGEASLAAFVAAEP
                     AALTGAELRGVLARHLPDYMVPTAVVVVDELPLTSNGKVDRQALLRLEPAPQEGAASP
                     AAPRTPDEDLMAGLWAQVLGVAQVGLDDNFFDLGGHSLLATRLIARVREIWQAEVSLV
                     DLFQGPTVAGLLTCLQAVAGGARDLLPPIERGPAGEDAPLSFTQQRLWFLDRLEPGNS
                     AFHIPVVLRATGPLHVTALERSFQELARRQEILRTTFPDVEGRPRQVVSPPAGPALPL
                     VDLSGLPEPQRAAETRRVAVAEARRAFSLASGPLWRLALLRLAAEEHVLLLVMHHIIS
                     DAWSMGILVREAVAFYSRFAEGKSCDLPALPIQYADFSVWQRRHLSGATLETLLDYWK
                     QRLGRPPAPLELPADGPRPAVPSYRGAHHHFRLSPELSSSLRELSRREGVTLFMTFLA
                     AFQVLLHRLSGRGDFVVGVDIANRNRAEIAGLIGFFVNQIAVRSNLSGEPTFRELLAR
                     VREDILGAYVHQDLPFDRLVDALQPERSLGHIPIFQVLLSFENTPKVTLAADRLVFAL
                     DDLDPGTAKRDLTLFVREDGASCAGSWNYSTDLFAPATIQRMAGQLEVLLGDIVAHPD
                     ARISSLEMRSEEERRRQDVEKLDRKDAKFANFLSVKPRAVGTAGKDLVRKQSLRDGQS
                     LPLLITPALDGVDLTAWVRANLEELESHLSRHGGVLFRGFDVKSVAEFEQFALAVCDP
                     LFGDYGDLPPEENGRKVYQSTPYPADKAILFHNEGSHTHRWPLKQFFFCLQAAQQGGE
                     TPIVDCRAMYNRLDPRLIDRLSRKQLLYVRNFTDGLDVSWQEFFKTEDRAEVERYCQR
                     AAIDFEWKENGLRTRHLSPAVVRHPKNGEMVFFNQVQLHHIACLEPAVRESLLEMYGE
                     EELPRNVYYGDGSPIEDSVMEEISRAYQETAVSFPWREGDIILLDNMLIAHGRNPFVG
                     PRRIVVAMGEMIEAPTVRG"
     gene            4053..>4666
                     /locus_tag="VEL74_06335"
     CDS             4053..>4666
                     /locus_tag="VEL74_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG62179.1"
                     /translation="MHDSGSAYRLSPQQRRIWSLQQETGAALAQAGVIVEGELDRDLL
                     RGVLARITEDHEILRTVFPTPQGMKQPAQSVLEHAGLSWSEAGCGSVPSEEISEAAQV
                     ASLLAEERSLGLGVARGPLLLVRLAALAPGRHLLVLTLPALCADSWTLEVLVREVAER
                     YAAAVARREWDDQPLPYIQFSEWQNDLIDGEAGEAGRAFWSRNG"
BASE COUNT          673 a         1573 c         1613 g          807 t
ORIGIN      
        1 gctggcgatc ctcaagaccg gagccgccta cgtgccgctc gaccccacct atccggccga
       61 gcggctggcg tacatgctgg gcgacagcgg cgcgccgctc ctgctggcgc gccgcgcgtg
      121 gcccggcggg gaaccggtcg ccggcgtcac gctcgtcgac ctggacgggt tggagctcga
      181 gggcgcggac gaggaggatc tggagatcga gatcccttcc gggtccccgg cctacgtgat
      241 ctacacctcg ggctcgaccg gccggccgaa gggggtcctg gtcacgcacg ccaacgtgct
      301 gcgcctcttc gcctcgacgc gggagctttt cggcttcggc agcaccgacg tgtggacgct
      361 cttccattcc tacgccttcg acttttcggt ctgggagatc tggggcgctc tgctccatgg
      421 cggccgtctg gtcgtggtcc ccttcgccgt cagccgttcg ccggagaagc tccatgcgct
      481 gctgcgccag gaggaggtca cggtcctcaa ccagaccccc tcggctttcc agcagctcgt
      541 ctgggccgag tccgagattc ggcagcggga ggggagcgcg ccccgtttgc cgcgcctgcg
      601 gtgggtgatc ttcggcggcg aggcgctcga cctcgcgcgg ctcgagccgt ggatcgagca
      661 ccatggcgac cggcggccgg agctggtcaa catgtacggc atcaccgaga cgaccgtgca
      721 cgtgacgcag cgccggatcc gccgggagga tctgtctgcc ggcggcagcc tgatcgggcg
      781 gccgctcccg gacctctccg tctacctgct gaaccgcggt ggcggcttgg tgccgatcgg
      841 tgtgccgggc gaaatctggg tcggcggcgc gggcttgggg accggctatc tcggccgccc
      901 cggcctcacg gcagagcgct tcgttccgga cccgttcggc gccgaggccg gcggacggct
      961 ctaccggtcg ggggacctgg cgcgtcatct ggcgaaccgc gagctgcagt acctgggccg
     1021 catcgaccat caggtcaagg tccgcggctt ccgcatcgag ctgggggaga tcgagaacgt
     1081 gctgcgccgg cacccgggcg tgcgtgatgt ggcggtgctg gtgcgccccg gcgctctggg
     1141 cgaggcaagc ctcgccgcct tcgtcgcagc cgagccggcg gccctgacgg gcgcggagct
     1201 gcgcggtgtg ctcgcacgcc acctgccgga ctacatggtg ccgaccgcgg tggtggtggt
     1261 agacgaactc cccctgactt cgaacggcaa ggtcgaccgc caggcgctcc tgcggctcga
     1321 gcccgcgccg caagaggggg ccgcgagccc ggcggcgccg cgtacccccg acgaggatct
     1381 gatggccggc ctgtgggcgc aggtgctggg ggtggcgcag gtcggtctcg acgacaactt
     1441 cttcgatctc ggtggccact cgctgctggc gacgcggctg atcgcgcggg tgcgcgagat
     1501 ctggcaggcc gaggtgtcgc tggtcgatct cttccagggg ccgaccgtcg ccggcctgct
     1561 cacctgtctc caggccgtcg cgggaggcgc gagggatctc ctgccgccga tcgagcgcgg
     1621 gccggcgggc gaggatgcgc cgctgtcgtt cacccagcag cgcctctggt tcctcgatcg
     1681 cctggagccc ggcaactccg cctttcacat tcccgtcgta ctgcgcgcca cgggtccgct
     1741 ccatgtcacg gccctcgaac ggagcttcca ggagctggcc cggcggcagg agatcttgcg
     1801 caccacgttc ccggacgtcg agggccggcc tcggcaggtg gtctccccgc ccgccgggcc
     1861 agccttgccg ctggtcgacc tctcggggct gccggagccg caaagggcag ccgagacgcg
     1921 gcgggtcgct gtagccgagg cgcggcgcgc cttcagcctg gcgtccggtc cgctctggcg
     1981 gctggcgctg ctgcggctcg cggctgaaga gcacgtcctg ctcctggtca tgcaccacat
     2041 catctccgac gcctggtcga tggggattct ggtccgggag gccgtcgcct tctactcccg
     2101 gtttgcggag gggaagagct gcgacttgcc agcgctgccc atccagtacg ccgacttctc
     2161 cgtctggcag cgccgtcatc tgtcgggcgc gactctcgag acgcttctgg actactggaa
     2221 acagcgtttg ggacgtcctc cggcgccgct ggagctgccc gctgacgggc ctcgtcccgc
     2281 cgtgcccagc tatcgcggtg cgcatcacca cttccgcctg tcgcccgagc tgtccagctc
     2341 cttgcgcgag ctcagccggc gtgaaggggt caccctcttc atgacctttc tggcggcctt
     2401 ccaggtgttg ctgcaccgtc tctcaggccg cggcgacttc gtcgtcggcg tcgatatcgc
     2461 caatcgcaac cgtgccgaga tcgcggggct catcggcttc ttcgtcaacc agatcgccgt
     2521 gcgcagcaac ctctctggtg agcccacctt ccgcgagctc ctggcgcggg tacgcgagga
     2581 catactcggc gcctacgttc atcaggacct gccgttcgac aggctggtgg acgcgctgca
     2641 acccgaacga agcctgggcc atattccgat tttccaggtg ctcctctcct tcgagaacac
     2701 gccgaaggtg acgctggcgg cggaccgcct cgtctttgca ttggacgatc tcgacccggg
     2761 caccgccaag cgcgatctca ctctcttcgt gcgcgaggac ggggcgagct gcgcgggatc
     2821 ctggaactac tcgaccgacc tctttgcccc ggcgacgatc cagcggatgg ctgggcagct
     2881 cgaggtcctg ctcggcgaca tcgtcgccca tccggacgcg cggatctcct cgctggagat
     2941 gcggagcgaa gaggaaagaa ggagacaaga cgtggagaaa cttgatagga aggatgcgaa
     3001 gttcgcgaat tttctgagcg tcaaaccacg agccgtcggc accgcgggca aggacctggt
     3061 ccggaagcag agcctgcggg acgggcagag cctgccgttg ctgatcactc cggctctcga
     3121 cggagtcgac ctgacagcct gggtacgggc caacctcgaa gagctcgaat cgcatctttc
     3181 acgtcatggc ggcgtcctct tccgcggctt cgacgtgaag tcggtcgccg agttcgagca
     3241 gttcgccctc gccgtctgcg atccgttgtt cggcgactat ggcgacctgc cgccggaaga
     3301 gaatggacgg aaggtctatc agtcgacccc ttatccggcg gacaaggcga tcctctttca
     3361 caacgagggc tcgcatacgc atcgctggcc cttgaagcag tttttcttct gtctccaggc
     3421 ggcgcagcag ggaggcgaga cgccgatcgt cgattgccgc gccatgtaca accggctcga
     3481 tccgcggctc atcgatcggc tctcccgcaa gcagcttctg tacgtccgga acttcaccga
     3541 cggtctggac gtgagctggc aggagttctt caagaccgag gaccgggcgg aggtcgaaag
     3601 gtactgccaa cgtgccgcca tcgacttcga gtggaaggag aacgggctgc ggacccgcca
     3661 cctcagtccg gcggtcgtgc ggcatccgaa aaatggcgaa atggtcttct tcaatcaggt
     3721 gcagctccat cacatcgcat gtctcgagcc tgcggtccgg gagtccctcc tcgagatgta
     3781 cggcgaagag gaactgccgc gcaacgtcta ctacggcgac ggctcgccga tcgaggactc
     3841 ggtgatggaa gagatttccc gcgcttacca ggagacggcg gtgagcttcc cctggcggga
     3901 gggggacatc atcctgctcg acaacatgct gatcgctcac gggcgcaacc cgttcgtggg
     3961 gccgcgccgg atcgtcgtgg ccatgggcga gatgatcgaa gctccgacgg tccgcggttg
     4021 aaggtctgac ggcgctcgag caaggaggtt ttgtgcacga cagcggcagc gcgtaccggc
     4081 tctctcctca acagcgacgc atctggtcgt tgcagcagga gacaggggcc gccctggctc
     4141 aggccggtgt gatcgtcgag ggggagctgg accgggatct cctgcgtggc gtcctcgccc
     4201 ggatcaccga ggaccatgag atcctgcgca ccgtcttccc gacgccgcag gggatgaagc
     4261 agccggcgca gagcgtgctg gagcacgccg gcctctcgtg gtcggaggcc gggtgcggtt
     4321 ccgtgccctc cgaggagatc tcggaggccg cgcaggtggc gtcgctgctg gccgaagaga
     4381 ggagcctcgg cttgggcgtc gcgcggggtc cgctgctgct cgtccggctc gccgctctcg
     4441 cgcccgggcg tcacctgctc gtcctgaccc tgcccgcgtt gtgcgccgac tcctggacgc
     4501 tcgaggtcct cgtgcgcgag gtcgccgaac gctatgccgc ggccgtcgcg cgacgggagt
     4561 gggacgacca gcctttgccc tacatccagt tctccgagtg gcagaacgac ctgatcgacg
     4621 gggaggctgg agaggccggc cgggctttct ggagccgcaa cgggtt
//
LOCUS       DASPJI010000238         4349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_644829, whole genome shotgun sequence.
ACCESSION   DASPJI010000238 DASPJI010000000
VERSION     DASPJI010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644829"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VEL74_06340"
     CDS             <1..498
                     /locus_tag="VEL74_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG62180.1"
                     /translation="SRFTVRLPAVHELGAPPPVGREVVEGRPAAVQEKGWGEGLHILL
                     IEDHADTAAAMADLLRAAGNEVTVANCLAEGREAAAAAQADGARRIDLVVSDVGLPDG
                     TGLDLMRELAGRYGLRGIALSGYGMEEDVRKSREAGFERHLTKPVDVQALRAAISEVA
                     GAGSS"
     gene            642..905
                     /locus_tag="VEL74_06345"
     CDS             642..905
                     /locus_tag="VEL74_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006099739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HYG62181.1"
                     /translation="MRLYVAGPTPRSIAAFSNLKRICEEHLAGRYRIELVDVLEDPRL
                     ARRDRVFAIPTVICERRGVAKKSATGDLSDTERVLAALNLPRA"
     gene            1100..3409
                     /locus_tag="VEL74_06350"
     CDS             1100..3409
                     /locus_tag="VEL74_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYG62182.1"
                     /translation="MLAAIVDSSDDAIIGKSLDGTILSWNSAAVRMYGYSAEEVIGRP
                     VSILTPPERRNEMHRILEAIRRGDKVDHFETERIAKDGRRLRISLTVSPIRDRQGRIV
                     GASKIARDIGDRKRAEDEAERARRRLFEFLDNLDEGFFILGPDWRFLYLNQVALRLSD
                     ESAEELVGRTLWEAFPALIGTETEAVYRRVMDTRLPERFETRGLRSGRWFEVRVYPSL
                     DGLSIFSRDFTDRKRAEDELRRKNEDLQAANEELQVAEEELRLSESALRQSEERFRDL
                     ADAVPSFVWTSGPDGLIGFLNERWYAYTGLRPEQSLGTGWVQALHPEDVERSLDAWEE
                     ARRRGDFYEVEMRYRRADGEYRWFIARARPVRDDRGRIVAWFGSSHDVHDRKLAEEAK
                     DRFLATLSHELRTPLTPVLAVTSNLEGDARLPADAREALSMVRRNVELEARLIDDLLD
                     LTRIARGKIELHPEAADARRILEHALETSARQIASKALRLETDLAAGDHRVWADSSRL
                     TQVFWNLLTNAVKFTPAGGTVRVRSWTEEDLERSDWLAVEVVDDGIGIEPELLPRIFD
                     AFEQGERGITRRYGGLGLGLAVSKAIIDLHGGRISAASAGPGRGAAFTVRLPVREQTA
                     PAVAAVPAATGLEAAERSLHVLLVEDHDDTAEAMADLLRASGHRVTVADTVAAALEAA
                     AAAANGSAAGPLDFVVSDLGLPDGNGHDLMRELARRHGLRGIALSGYGMEEDVRRSRE
                     AGFSRHLTKPVSPQLLRSVLREAAEAGGG"
     gene            3406..>4349
                     /locus_tag="VEL74_06355"
     CDS             3406..>4349
                     /locus_tag="VEL74_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HYG62183.1"
                     /translation="MRVFRRDRLRRQEQVLVELAKSRAAERGDLAAALRELTEAAAET
                     LEVERVGVWIYTEDRFRIRCIELFERTPGRHAAGAELAAADFPAYFKALEQERTLAAS
                     DARRDERTREFTTSYLEPLGIGAMLDAPLRAHGRMIGMICHEHVGGERRWSAGEQAFA
                     GSIADLAALALEAEERNRAERELRERVAELQILMSILQESEERSRLANEAKDHFLATL
                     SHELRTPLTPVLAMASMLERDERLPGDARNALTMMRRNVELEARLIDDLLDLTRISRG
                     KLELERGAADVRQILEHALDTVEAELMTKRLRLVTDLE"
BASE COUNT          689 a         1486 c         1544 g          630 t
ORIGIN      
        1 tcccgattca ccgtgcgcct gcccgccgtc cacgagttgg gcgcgccgcc gcccgttggc
       61 agggaggtgg tggagggacg gcccgcggcg gtccaggaaa aaggctgggg agagggcctc
      121 cacatcctgc tgatcgagga ccatgccgat accgcggcgg ccatggccga cctgctgcgg
      181 gctgcgggga acgaggtcac ggtcgccaac tgcctggccg aggggcgcga ggcggccgcg
      241 gccgcccagg ccgacggagc gcggcggatc gacttggtgg tcagcgatgt gggcctgccg
      301 gacggtaccg gcctcgacct gatgcgcgag ttggccggcc gctacggcct gcggggtatc
      361 gccctctcgg gctacggcat ggaggaggac gtccgcaaga gccgggaggc cggcttcgag
      421 cgccacctca ccaagccggt ggatgtccag gcgctacggg cggcgatctc cgaggtcgcc
      481 ggcgcgggta gctcgtagcg cccgtttcgc ctgcacttgc caaataccat tttttctcta
      541 caatccttct gacgtatcgg ctggtctcat tgcaggaggt ccttgtgctc cagaaacgga
      601 agccacccct tccgactccc ccgagcgccc aagactggga cttgcgcctg tatgtggcag
      661 ggccgacccc caggtcgatc gcggccttct ccaacctcaa gcggatctgc gaggagcatc
      721 tggccggccg gtaccggatc gagttggtgg acgtgctcga agaccccagg ctggcacggc
      781 gggacagggt cttcgccatt ccgacggtta tctgcgagcg gcggggagtg gcgaagaaga
      841 gtgcgaccgg agacctctcg gacaccgagc gggtcctcgc ggccttgaac ctgccgcggg
      901 cctgagcccc gcgcggagga gcgccgggcg cagccgatcg gctacactgc tcaggacagt
      961 caggaccacg ggcgcatgca ccttctgcat gttgcaagct ccggagtgcg ccagccaccg
     1021 aatgacctcc gcaggacctt tcagaaagcg ccccccccag ccggaggccg gagctacgcc
     1081 tctccccgag acggaggcat tgctggcggc gatcgtcgat tcctccgacg acgccatcat
     1141 cggcaagagc ctcgacggca ccatcttgag ctggaactcc gcggcagtgc ggatgtacgg
     1201 ctactcggcc gaggaggtca tcggccggcc ggtatcgatc ctcaccccgc ccgagcgcag
     1261 aaacgagatg caccgcatcc tggaggccat ccggcgcgga gacaaggtcg atcacttcga
     1321 gaccgagcgt atcgccaagg atggacgccg cctccggatc tcgctgaccg tctcgccgat
     1381 ccgcgatcgg cagggccgga tcgtcggcgc ctcgaagatc gcccgcgaca tcggcgaccg
     1441 caagcgggcc gaggacgagg ctgagcgagc ccgccgccgg ctcttcgagt tcctggacaa
     1501 cctcgacgag ggcttcttca tcctgggccc ggactggcgt ttcctctacc tcaatcaggt
     1561 cgctctgagg ctgtccgacg agagcgccga ggagctggtg ggccgcacgc tctgggaggc
     1621 gttccccgct ctgatcggca cggagaccga ggctgtctat cgccgggtga tggacacccg
     1681 cctccccgag cgcttcgaaa cacgcggtct gcgctccggc cgctggttcg aggtgagagt
     1741 ctatccatcc ctggatgggc tgtccatctt ctcccgcgac ttcaccgacc gcaaacgggc
     1801 cgaagacgag ctgcgccgta agaacgagga cctacaggcc gccaatgagg agctgcaggt
     1861 cgcggaagag gagctacgcc tatccgagtc ggcgctgcgg cagagcgagg agcgattccg
     1921 cgacctggcg gacgccgtgc cctcgttcgt ttggacctct gggcccgacg gcctgatcgg
     1981 cttcctcaac gagcgctggt acgcctacac cggcttgaga cccgagcagt cgctgggcac
     2041 cggctgggtg caggcgctgc atccggagga cgtcgaacgc tccctcgatg cctgggagga
     2101 ggcgcggcgg cggggtgact tctatgaagt ggagatgcgc taccgccggg ccgacggcga
     2161 gtatcgctgg ttcatcgccc gagcccgccc ggtccgggac gacagaggcc gtatcgtcgc
     2221 ctggttcggg agcagccacg acgtccacga ccgcaagctg gcggaggagg ccaaagaccg
     2281 cttcctggcc accctgagtc acgagctgcg gactccgctg acaccggttc tggcggtcac
     2341 ctcgaacctc gaaggggatg cccggctgcc ggcggacgcg cgagaggccc tgtccatggt
     2401 gcggcgcaat gtagagctgg aggcccgtct gatcgacgac ctcctggacc tgacgcgtat
     2461 cgcccggggt aagatcgagc tccaccccga agcggcggac gcgcgccgga tcctggagca
     2521 cgccctggag acctccgccc ggcagatcgc ctccaaggcg ttgcgcctgg aaaccgatct
     2581 ggcggccggc gatcaccgcg tctgggctga ttcctcccgc ctgacccagg tcttctggaa
     2641 cctgctcacc aacgcggtca agttcacgcc ggctggcggc acggtgcgag tgcggtcgtg
     2701 gaccgaggag gaccttgaac ggtccgactg gctcgcggtc gaggtggtag acgacggcat
     2761 cggcatcgag ccggagctgc tgccgcggat cttcgacgcc ttcgagcagg gcgagcgcgg
     2821 catcacgcgc cgctacggcg gcctggggct gggtctcgcc gtcagcaagg cgatcatcga
     2881 cctgcacggc ggccggatct ccgccgccag tgccgggccg ggccggggag cggccttcac
     2941 cgtccgtctg cccgtgcgcg agcagacggc cccggccgta gctgccgtcc cggccgccac
     3001 cggcttggag gcagcggagc gatcgctcca cgtcctcctg gtggaggacc acgacgacac
     3061 ggcggaggcc atggccgacc tgctgcgggc ctcgggccac cgggtgacgg tggcggatac
     3121 ggtggcagcg gcactggagg ccgctgccgc ggcggcgaat ggcagcgcgg ccggcccgct
     3181 cgatttcgtg gtgagcgacc ttgggctgcc cgacggcaac ggtcacgacc tgatgcgaga
     3241 gctggcccgc cgtcacggtc tacgggggat cgccctgtcc ggctacggca tggaggagga
     3301 cgtccgccgg agccgggagg ccggtttcag ccggcacctg acgaagccgg tcagcccgca
     3361 gctcctgcgc tcggtgctgc gggaagcggc ggaggcaggg ggcggatgag agttttccgg
     3421 cgagaccgcc tgcggcggca ggagcaggtt ctcgtcgagc tggccaagag ccgcgcggcc
     3481 gagcgtggcg acctcgccgc cgccctccgc gagctgaccg aggcggcggc cgagaccctc
     3541 gaggtcgagc gcgtcggcgt ctggatctac accgaagacc gctttcggat ccgctgcatc
     3601 gagctcttcg aacgcacccc cggccgtcac gccgcggggg ccgagctggc cgcggccgac
     3661 ttcccggcct atttcaaggc tctggagcag gagcgcactc tcgcggccag cgacgcgcgc
     3721 cgcgacgaaa ggacacggga gttcaccacc tcctacctgg agccgctcgg tatcggtgcc
     3781 atgctcgatg cccccctgcg ggcgcacggc cggatgatcg gcatgatctg tcacgagcac
     3841 gtcggaggcg agcgcaggtg gagtgcgggc gagcaagcgt tcgccggctc gatcgccgat
     3901 ctggccgccc tcgccctcga agccgaggag cgcaaccgcg cggagcggga gctgcgggag
     3961 cgcgttgcgg agctccagat cctgatgagc atcttgcagg agagcgagga gcgctcgcgg
     4021 ctcgccaacg aggccaagga ccacttcctc gccaccctga gccacgagct gcgcacgccc
     4081 ctgacgccgg tcctggcgat ggcgtccatg ctcgaacgcg acgagcggct gccgggcgac
     4141 gcgcgcaacg ccctcaccat gatgcggcgc aacgtcgagc tggaggcccg gctgatcgac
     4201 gacctgctgg acctcacccg catctcgcgc ggcaagctgg agctggagcg cggcgcggcg
     4261 gacgtgcgcc agatcctgga gcacgccctg gacacagtgg aggcggagct gatgaccaag
     4321 cggctgcgcc tggtgaccga cctggagat
//
LOCUS       DASPJI010000239         6120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_247751, whole genome shotgun sequence.
ACCESSION   DASPJI010000239 DASPJI010000000
VERSION     DASPJI010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247751"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VEL74_06360"
     CDS             complement(<1..1066)
                     /locus_tag="VEL74_06360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG62184.1"
                     /translation="MTADPRPPQLTYIHGAPPEGLKLSVVIPVYNERFLVAELVRRVL
                     AVEVPGLREMELVIVDDGSRDGTRDILRGLAAAHPGRIHYIEHERNQGKGAAIRTGIA
                     ASTGDLILFQDADLEYDPRDYPRLVRPFIEDGADVVYGSRFLPSERRRVLFHRHSLGN
                     RFLTVLSNWFTDLSFTDMETCYKMFRAPLLKSIPIRSNDFAMEPEITAKIAKRDCRIF
                     EVPISYLGRTYREGKKIGWKDGFKALRAMVRYWLIDDLYAEDEYGSHILHSLEKAQRF
                     NRWMAESIAPYVGARVLEIGAGIGNITTWLLPRDVYVASDINPNYLHYLKNFSLGKPY
                     LQVDRIDLEDSACFERWQGQF"
     gene            complement(1063..2058)
                     /locus_tag="VEL74_06365"
     CDS             complement(1063..2058)
                     /locus_tag="VEL74_06365"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYG62185.1"
                     /translation="MKREFVESMRCPFCRNRLGLTVGEEDAREVREGRLDCSCGRSFP
                     VHKGIPDFIDPTDETLAREVAGWIQLAGNLDEGLVPVMTALPQYPHPPWPHVAPDFFQ
                     IFEHLSFAGQNVVDIGAGRTWSSRFLKKLGLAKEVVAVDVLTTRFLGLETADIFFEAD
                     GMQFERVRGDIHRLPLHDNWADAVFSCAAIHHSGDLDSLFAEVFRVLGPGGAFVFISE
                     PVKMESIPGNRPNNIETELGINEHFYSYREYKEALERAGFVFHRMVPRSLGYRMLYAD
                     PELVHDAPGPIQRFSMTPRGRKGLDLLLRNRFAGPWLYRKWSLPLSGIAQKPRPA"
     gene            complement(2095..4389)
                     /locus_tag="VEL74_06370"
     CDS             complement(2095..4389)
                     /locus_tag="VEL74_06370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG62186.1"
                     /translation="MTRLAVVIAAYDEEETVEVLTRRLHRVLSGLPDCAWEILYVVEG
                     RDRTREILAGLAGELERIRVLYQEEPSGLGNAFRRGFAAALEGNPDLVVTMDADLNHQ
                     PEEIPHLLEAFRRHGCDILVGSRFLAASRVEGTPAWKRFLSGTTNVLMRRLYGLRVRD
                     KTSGFRVYRAAALRSLSFENANFAFLPELLIRAHQAGMTLAEEPIHFIFRREGQSKMR
                     FWATSFSYLKLLRTRFDRWSLAALMLLLTGLVVRAVMAFPSHKYPADADAILSGLCAF
                     KILRGDLPVFFSAARIGAIECYLAAGAFLVTGPSRAALMAVALAFGLVFLVASYLFAR
                     ALLGRRIACLALLFLILPSPSVLFWTYVLNGYPAMMASCAVILWLAVRLGRKPIPAPG
                     EGRRTAFLLGLAAGVGLWHSIQTLGVTAPAGLWLLGYRRGLLRDRRLVALAVAGAVLG
                     TAPLIAYNIKHPLGSLRGNYAARPPDGGLRALAENGTYIVTYNVPELLASVNPQGFLA
                     PPVPARLRPAVLAVYAAAFLFLLALPLLRRRGSGLAAGVPPDAWALLLLVVLSVLGLN
                     LVSAAGQVRGLTVRYVLPIAIVAAPILAVFVHAVARRSRAAAAVLAACVAALGLAGYP
                     WPWTAERQVWAGRATADERLLDTLASRRIQVVAGGYWTVYPVAFLSRERIVPHPCEDE
                     HDHYQYARWLQPHPQRWALLAWANPDGRPTWIHEMRRRTGLAGTLETGIPGYVVFFPA
                     ENPPRQRPAEFLTRMRSVCEARPY"
     gene            complement(4386..5066)
                     /locus_tag="VEL74_06375"
     CDS             complement(4386..5066)
                     /locus_tag="VEL74_06375"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG62187.1"
                     /translation="MSHGEREAWDQHWRELEGGDRSLFGTLASLVRKTILCRAVRHYT
                     DRYFPPRGLFVEPGCGTAQASARVAPLERGLVAFDFSLPALAAARGSAVHRHFVCGDI
                     RRLPFRDGSVAGIWNLGVMEHFHPGDGREILEEFRRVLAPRGCAILFWPPEFGLSRWV
                     LAPFEWIRGKSTGKPFQFFPDEVNRLRSLRHARETLRGGGLEPVAADFTPRDSFIHVV
                     AVGRKPAA"
     gene            5242..>6120
                     /locus_tag="VEL74_06380"
     CDS             5242..>6120
                     /locus_tag="VEL74_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYG62188.1"
                     /translation="MQKNLLRSARPWRDGASLPLPALPLLSILLLAMLLGSGAVSAQV
                     PNTPPSFTGFPITLAGEGNLEYSQPVAADLGTLAGGTAGYLEIVFGTKQGDLHVIHRN
                     TNGTWAEAPGFPVNVGTYIASSPAVGDLTGDGVPEIVVGHGNHNVNGGGGPGGVKAFR
                     NNGSLLWARSSFDYVTGQDGQPDAVFGTPAIGDVDGDGQNEVAWGGFDFRIHLVSGAN
                     GSYEPGWPLFIRDTVWSSPALHDIDGDGEKDIIIGVDAHDEPGFGTQNGGSLHVLRYD
                     ASQTCSTANPESCTMPT"
BASE COUNT          997 a         2132 c         2171 g          820 t
ORIGIN      
        1 cgaactgccc ctgccaacgc tcgaaacagg ccgagtcctc cagatcgatg cggtctacct
       61 ggagataggg tttacccagc gagaagttct tcaggtagtg caggtagttc ggattgatgt
      121 cgctcgccac gtagacgtcc cgcggcagca gccaggtcgt gatgttgccg atcccggcgc
      181 cgatctccag cacccgcgct ccgacgtagg gggcgatcga ctcggccatc cagcggttga
      241 agcgctgcgc cttctccagg ctgtgcagga tgtgcgagcc gtactcgtcc tcggcgtaga
      301 ggtcgtcgat cagccagtag cggaccatcg cccgcaacgc cttgaagccg tccttccagc
      361 cgatcttctt gccctcgcgg taggtgcggc ccaggtagct gatcggcacc tcgaagatcc
      421 ggcagtcgcg cttggcgatc ttcgccgtga tctccggctc catggcgaag tcgttggaac
      481 ggatggggat ggacttgagc agcggagccc ggaacatctt gtagcaggtc tccatgtcgg
      541 tgaagctcag gtccgtgaac cagttgctca gcacggtcag gaagcggttg cccagcgagt
      601 gccggtggaa gagtacgcgc cgccgctcgc tcggcaggaa gcgcgagccg tagaccacgt
      661 ccgcgccgtc ctcgatgaag gggcgcacca ggcgcgggta gtcgcgcggg tcgtactcca
      721 ggtcggcgtc ctggaagagg atgaggtcgc cggtcgaggc ggcgatgccg gtgcggatgg
      781 ccgccccctt gccctggttc cgctcgtgct cgatgtagtg gatgcggccg ggatgagccg
      841 ccgccaggcc gcgcaggatg tcgcgcgtgc cgtccctgga gccgtcgtcg acgatcacca
      901 gctccatctc gcggaggccg ggcacctcca ccgccagcac ccgccgcacc agctccgcca
      961 ccaggaaacg ctcgttgtag acggggatca cgacggagag cttgagcccc tccggcggcg
     1021 ctccgtggat gtaggtgagc tgcggcgggc gcggatcggc ggtcatgcgg ggcggggttt
     1081 ctgggcgatg cccgagagcg gcaggctcca cttgcggtag agccacgggc cggcgaagcg
     1141 gttgcgcagc aggaggtcca gccccttgcg gccgcgcggc gtcatcgaaa aacgctggat
     1201 cgggcccggc gcgtcgtgga cgagctccgg atccgcgtag agcatccggt agccgagcga
     1261 gcgcggcacc atgcggtgga agacgaagcc cgcccgctcc agcgcctctt tgtactcgcg
     1321 gtagctgtag aagtgctcgt tgatccccag ctcggtctcg atgttgttcg gccggttgcc
     1381 ggggatcgac tccatcttca ccggctcgga gatgaagacg aaggccccgc ccgggcccag
     1441 caccctgaag acctcggcga acagggagtc caggtccccc gagtggtgga tcgcggcgca
     1501 gctgaagacg gcgtcggccc aattgtcgtg cagcggcagc cggtggatgt cgccgcgtac
     1561 ccgctcgaac tgcatgccgt ccgcctcgaa gaagatgtcc gcggtctcga ggcccaggaa
     1621 gcgggtggtc agcacgtcca ccgccaccac ctccttcgcc aggccgagct tcttcaggaa
     1681 ccggctcgac caggtgcgcc cggcgccgat gtccaccacg ttctggccgg cgaagctcag
     1741 gtgctcgaag atctggaaga agtcgggcgc gacgtgcggc cacggcgggt gcgggtactg
     1801 gggcagcgcc gtcatcaccg gcaccagacc ctcgtccagg ttgccggcga gctggatcca
     1861 gccggcgacc tcgcgggcca gcgtctcgtc ggtgggatcg atgaagtcgg ggatgccctt
     1921 gtggaccggg aacgaccggc cgcaggagca gtcgagccgc ccctcgcgga cctcgcgcgc
     1981 gtcctcctcc cccacggtca ggccgaggcg gttgcggcag aacgggcacc tcatggattc
     2041 gacgaattct ctcttcatgc gcgctcccgc ggacggggcg agagcctatc acccctaata
     2101 aggccgtgct tcacacaccg agcgcatccg cgtcaggaac tcggccggcc gctggcgggg
     2161 cgggttctcc gccgggaaga agacgacata gccggggatg ccggtctcga gcgtgccggc
     2221 gagcccggtc cgccgccgca tctcgtggat ccaggtgggc cgcccgtcgg ggttcgccca
     2281 ggcgaggagg gcccagcgct gcgggtgcgg ctgcagccag cgggcgtact ggtaatggtc
     2341 gtgctcgtcc tcgcaggggt gggggacgat ccgctcccgg gacaggaagg ccaccgggta
     2401 gacggtccag tacccgccgg cgaccacctg gatgcgccgc gaggccaggg tgtccaggag
     2461 ccgctcgtcc gccgtggcgc ggccggccca tacctggcgc tccgcggtcc agggccaggg
     2521 atacccggcc aggccgagag ccgcgacgca ggcggcgagg accgccgcgg ccgcgcggct
     2581 ccggcgtgcc acggcgtgca cgaaaacggc gaggatgggc gccgccacga tggcgatggg
     2641 caggacgtag cgcacggtca gcccccgcac ctgccccgcc gccgagacga ggttcagccc
     2701 caggaccgag agcaccacca gcagcaggag cgcccaggcg tccggaggga cccccgcggc
     2761 cagcccgctc ccccgccgcc gcaggagggg cagcgccagg aggaagagga aggccgcggc
     2821 gtagaccgcc agcaccgccg gccgcaggcg cgccggcacc ggcggcgcga ggaagccctg
     2881 cgggttcacc gaggccagca gctccggcac gttgtaggtg acgatgtagg tcccgttctc
     2941 ggccagcgcc ctcagcccgc cgtccggcgg ccgggccgcg tagttgcccc gcaggctgcc
     3001 aagaggatgc ttgatgttgt aggcgatcag cggcgccgtg ccgaggaccg cccccgccac
     3061 ggccagggcg acgaggcgcc ggtcgcgcag caggccgcgc cggtagccga gcagccagag
     3121 cccggccggc gccgtgacgc cgagggtctg gatcgagtgc cagagcccca ctcccgccgc
     3181 gagccccagg aggaaggcgg tccgcctccc ctcccccggc gccggtatcg gctttcgtcc
     3241 cagacgcacc gccagccaga ggatcacggc acaggacgcc atcatcgccg gatagccgtt
     3301 cagcacgtag gtccagaaga ggaccgacgg gctgggcaga atcaggaaga ggagcgccag
     3361 acaggcgatg cggcgcccca gcagcgcgcg ggcgaagagg taggacgcca ccaggaacac
     3421 caggccgaag gccagggcga ccgccataag ggctgcccgg gacggtccgg tcacgaggaa
     3481 ggccccggcc gccaggtagc actcgatggc gccgatccgg gcggcggaga agaagaccgg
     3541 gaggtctccg cgcaggatct tgaaggcgca gagcccggag aggatggcgt cggcgtccgc
     3601 cgggtacttg tgggagggga aggccatgac cgcccgcacc accagaccgg tgaggagcag
     3661 catcagggcc gccaggctcc agcggtcgaa gcgcgtccgc agcagcttca gataggagaa
     3721 gctggtggcc cagaagcgca tcttggactg cccctcgcgc cggaagatga agtggatcgg
     3781 ctcctcggcg agggtcatcc cggcctgatg ggcgcggatc agcagctcgg ggaggaaggc
     3841 gaagttagcg ttctcgaacg acagggagcg cagggccgcg gcccggtaga cgcggaagcc
     3901 ggaggtcttg tcgcgcaccc gcaggccgta gagccggcgc atcagcacgt tggtggtgcc
     3961 ggagaggaag cgcttccagg ccggcgtccc ctccacccgg ctcgccgcca ggaagcgcga
     4021 ccccaccagg atgtcacacc cgtggcgccg gaatgcctcc agcaggtggg ggatctcctc
     4081 cggctggtgg ttgagatcgg cgtccatggt gaccaccagg tcggggttgc cctctagggc
     4141 ggcggcgaag ccgcgccgga aggcgttgcc caggccgctg ggctcctcct ggtagaggac
     4201 gcggatcctt tccagctcgc cggccagccc ggcgaggatc tcgcgcgtcc ggtcccgccc
     4261 ctccaccacg tagaggatct cccaggcgca gtcgggcagc ccggacagca cccggtggag
     4321 gcgccgggtg agcacctcga cggtctcctc ctcgtcatag gcggcgatga ccaccgcgag
     4381 ccgcgtcatg ccgcgggctt cctcccgacc gccacgacgt ggatgaagga gtcgcgcggg
     4441 gtgaagtccg ccgcgaccgg ctcgagccca cccccgcgca gggtctcccg ggcgtggcgc
     4501 agcgaccgca gccggttcac ctcgtcggga aagaactgga agggcttccc cgtcgacttg
     4561 ccgcggatcc actcgaacgg cgcgagcacc cagcgcgaca ggccgaactc cggcggccag
     4621 aagaggatgg cgcagccgcg cggcgccagc acccgccgga actcctcgag gatctcccgg
     4681 ccgtcgccgg gatggaagtg ctccattacc cccaggttcc agatgccggc caccgagccg
     4741 tcgcggaacg gcagccgccg gatgtcgccg cagacgaagt ggcggtgcac cgccgagccg
     4801 cgggcggcgg cgagcgccgg cagcgagaag tcgaacgcga ccagcccccg ctccagcggc
     4861 gcgacccggg ccgacgcctg ggcggtgccg catcccggct cgacgaacag gccgcgcggc
     4921 gggaagtagc ggtccgtgta gtggcggacc gcccggcaga ggatggtctt gcgcaccagg
     4981 gaggccagcg tcccgaagag ggaccggtcg cccccttcga gctcccgcca gtgctggtcc
     5041 caggcttcgc gctcgccgtg actcattcaa ggatctccag gcacacgtga aagcggcaga
     5101 tcatctaatg gtatcgcctt cagcgcaagc gccgtcccct cccccaggag actcacccgg
     5161 aatccggggg gccgggtcct ggttctgtga gaggacctct gcactgatag ccgagcttct
     5221 atgttaggat acgcacgctc tatgcagaaa aatctcttgc ggtccgcgcg gccttggcgc
     5281 gatggcgcca gcctccctct gcccgccctg cccctcctct cgatccttct cctggccatg
     5341 ctcctgggca gcggagctgt gtccgcccag gttcccaaca cgccgcccag cttcacgggc
     5401 ttccccatca ccctggccgg cgaggggaac ctcgagtaca gccagccggt ggcggcggac
     5461 ctcggcactc tggccggggg cacggcgggg tacctggaga tcgtcttcgg caccaagcag
     5521 ggggacctgc acgtcatcca ccgcaatacc aacggcacgt gggcggaggc gcccggcttt
     5581 ccggtgaacg tcggcaccta catcgcctcc agcccggcgg tgggcgacct gaccggcgac
     5641 ggcgtgccgg agatcgtggt cgggcacggc aatcacaacg tcaacggcgg cggcgggcca
     5701 ggcggcgtca aggcctttcg caacaacggc tccctgctct gggcgcgcag cagcttcgac
     5761 tacgtcaccg gccaggacgg ccaacccgat gcggtcttcg gcacgccggc catcggcgac
     5821 gtggacggcg acggccagaa cgaggtggcc tggggcggct tcgacttccg gatccacctg
     5881 gtgagcggcg ccaacggcag ctacgagccg ggctggccgc tcttcatccg cgacaccgtg
     5941 tggtcctcgc ccgccctgca cgacatcgac ggcgacggcg agaaggacat catcatcggc
     6001 gtcgacgccc acgacgagcc cggcttcggc acccagaacg gcggcagcct gcacgtgctg
     6061 cgctacgacg cgtcgcagac ctgctccacg gcgaaccccg agagctgcac catgcccacc
//
LOCUS       DASPJI010000240         4298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_819304, whole genome shotgun sequence.
ACCESSION   DASPJI010000240 DASPJI010000000
VERSION     DASPJI010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819304"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VEL74_06385"
     CDS             complement(<1..187)
                     /locus_tag="VEL74_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62189.1"
                     /translation="MALVESMESRLGILMLDGKMADVAGCMASSATFPYPVVRRVVPG
                     AGPPGSAADVEALLPLYL"
     gene            complement(198..914)
                     /locus_tag="VEL74_06390"
     CDS             complement(198..914)
                     /locus_tag="VEL74_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62190.1"
                     /translation="MTSRPPLGILMLEGKMADVPGCMACEATFPYPVVRKVVRGSRTP
                     LSAEEAWAMLPLYVDAARELEREGVAAITANCGLIALMQKELAATVSVPVVTSALLMV
                     PDLHRLIGGRRIGILTFFTHAVSERNYNASGWSSQDIPVALGGVGEHESWLTFLRTKE
                     VSPELRERLTPDLLGVVRGMLAEHPDIGAFVSECTMLPACLQTVREEVGVPVFDILTQ
                     LDLAMSGSYRPADVRAAVAA"
     gene            complement(918..1925)
                     /locus_tag="VEL74_06395"
     CDS             complement(918..1925)
                     /locus_tag="VEL74_06395"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HYG62191.1"
                     /translation="MTVSAAVATDLKVGSKAWVERHGLSAVETCRLAQLELARQDRRF
                     FSLEADLGDFGGFPFRDEFPDRYIDFGIAEASLVGAAGGLALRGKIPLINTFASFALM
                     RACEQVRLDLCYHKANAKIFGTFAGLQAGFSGPTHHCIEDVAIARGFPGMTVIAPADA
                     VSAYWATLAAGHHPGPVYIRLAVDVTAQVYGEDCPFEIGKGNTLRDGGDLTLVASGLT
                     VVAEALAAAEILAREGIDARVLDLHTIKPVDRELLATAARETGLLITVEEHTVHGGLG
                     GAVAEAVAQDAPVPLRILGIPDAYAEYVGPYTEHLRRYGLDAEGIAAAARQALTAKKG
                     R"
     gene            complement(1922..2788)
                     /locus_tag="VEL74_06400"
     CDS             complement(1922..2788)
                     /locus_tag="VEL74_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HYG62192.1"
                     /translation="MTKVSEIPQADRWADLKDIAHTLRHHIVHMAADSPGAHVGGSMS
                     VTEILTVLFFDGVLRVDPAQPKMDGRDYLIFSKGHASAALYAALAERGFFPVEELKTY
                     KQMGSRLAGHSLKSIPGVELATGSLGHGLPVGNGVALAGKHDGSSYRTFVVMGDGECQ
                     EGSVWEAAMAAAHYKLDNLVGIVDRNGVQEDGPTEQIMALEPFADKWRSFGWEVREVD
                     GHDVEDLSRALHAVPFVPGKPSLVLANTVKGKGVSFAEHAHSWHYGKLSGDQRDRALA
                     DLGPRRLREGRS"
     gene            complement(2833..4209)
                     /locus_tag="VEL74_06405"
     CDS             complement(2833..4209)
                     /locus_tag="VEL74_06405"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HYG62193.1"
                     /translation="MSLIEAERPAPRPVAGRLALHGGAPVVPPGTIVERWPLVEEEDI
                     AAVVDTLRSGRLSWINNDAVPALEQAWADYIGSRYCLAFNSGTAALHAAVAAAGVQPG
                     DEVLVPALTFLASATAVIHHQGLPVFVDIDPRTFTLDPAKIEERITDRTKAVVVVHLH
                     GLPADMDPILEIARRRGIKVIEDTCQAPGALYKGRKAGGLGDMAAYSIMAGKNLPTAG
                     EGGLFTTSDPALRDAADMIKMFGEKVRPDQAREYNALTMGWNYRFSSILAAFTLSQLE
                     RLDSYTERVQEGARRLARGLEEIPGLIPPFVPDDRTHVYHHFRFRVDPAAAGVDLPVG
                     VFRKAVQDAMEAEGVPLIEYQNRPIPGQLLFVERKGYGQGCPWTCGHTSRDVTYDMYE
                     YPQTLDVIRSTLLVGHRLCMASFMESRNVDLYLEAFQKVFSRLDDLTDHARILDYREP
                     WEQAARLW"
BASE COUNT          672 a         1484 c         1479 g          663 t
ORIGIN      
        1 cgagatagag cgggagaagg gcttccacat ccgcggcgga gccgggcgga ccggcgccgg
       61 ggaccacccg gcggaccacc ggatagggga aggtggcgct cgacgccata cagccggcga
      121 cgtccgccat cttcccatcg agcatcagga tgccgagccg ggattccatg gattccacca
      181 gcgccaccgg catcctccta cgcggccacc gcggcccgca cgtcggcggg acggtagctg
      241 ccgctcattg cgaggtcgag ctgagtcagg atgtcgaaga ccggtacccc cacctcctcg
      301 cgcaccgtct gcagacaggc cgggagcatc gtgcactcgg agacgaaggc gccgatgtcg
      361 ggatgctcgg cgagcatgcc gcggacgacg ccgaggaggt cgggggtgag ccgctcccgc
      421 agttccgggg agacctcctt ggtacgcagg aaggtgagcc acgactcgtg ctccccgacg
      481 ccgcccagag cgaccggaat gtcctggctg ctccaccccg aggcgttgta attgcgctcg
      541 ctcacggcgt gggtgaagaa ggtgaggatg ccgatccgac gcccgccgat caggcggtgg
      601 aggtcgggga ccatgagcag cgctgaggtc accaccggca cgctgacggt ggcggcgagc
      661 tccttctgca tgagagcaat gagcccgcag ttggcggtga tagccgcgac cccttctcgc
      721 tccagctcac gcgccgcatc aacgtagagg gggagcatgg cccatgcctc ttcggcggag
      781 agcggcgtgc gtgagccgcg caccaccttg cgcaccaccg ggtaggggaa cgtcgcctca
      841 caagccatac agccgggaac gtcggccatc ttcccttcga gcatcaggat gcccagcggc
      901 gggcggctcg tcatgcgtca ccgccccttc ttcgcggtga gcgcctgccg cgcggcggcc
      961 gcgatgcctt cggcatccag cccgtagcgg cgcaggtgct ccgtgtaagg cccgacgtat
     1021 tcggcatagg catcggggat gcccaggatg cgcaacggca ccggcgcgtc ctgcgccacg
     1081 gcctcggcca cggcgcctcc caggccgccg tgcaccgtgt gctcctccac cgtgatcagc
     1141 agcccggtct cgcgggccgc cgtggccagc agctcgcggt cgacgggctt gatggtgtgg
     1201 aggtcgagga cgcgcgcgtc gatgccctcg cgcgccagga tctccgccgc agcaagcgcc
     1261 tccgccacca cggtgaggcc ggaggccacc agggtcaggt caccgccgtc ccgcagcgtg
     1321 ttccccttac cgatctcgaa cgggcagtcc tcgccgtaaa cctgggcggt cacgtccacc
     1381 gccaggcgga tgtagaccgg gccggggtga tggcccgccg ccagggtggc ccagtaggcg
     1441 gagaccgcgt cggccggagc gatcaccgtc attccgggga agccccgcgc gatggctacg
     1501 tcctcgatgc agtgatgggt cgggccggag aaaccggcct ggagcccggc gaaggtgccg
     1561 aagatcttgg cgtttgcctt gtgatagcag aggtcgaggc gcacctgctc gcaggcgcgc
     1621 atcagggcga acgacgcgaa ggtgttgatc agcgggatct tgccgcgcag cgcgagaccg
     1681 cctgcggcgc cgaccaggct cgcctcggcg atgccgaagt cgatgtagcg gtccgggaac
     1741 tcgtcgcgga acgggaagcc gccgaagtcc cccaggtccg cttcgagaga gaagaaacgc
     1801 cggtcctggc gggcgagctc gagctgggct aggcggcagg tctccaccgc cgacagccca
     1861 tggcgctcca cccaggcctt gctgcccacc ttgaggtcgg tggcgaccgc tgccgacact
     1921 gtcatgaccg cccctcccgc agacgccgcg gcccgaggtc ggcgagcgcc cggtcccgct
     1981 ggtcgccgga cagcttgccg tagtgccagg agtgggcgtg ctcggcgaag gagaccccct
     2041 tgcccttgac cgtattcgcc agcaccagcg agggcttgcc cggcacgaac ggcacggcat
     2101 gtagcgccct ggacagatcc tcgacatcgt ggccatccac ctcgcgcacc tcccagccga
     2161 agctccgcca tttgtcggcg aagggctcga gcgccatgat ctgctcggtg gggccgtcct
     2221 cctgcacgcc gttgcggtcg acgatgccca ccaggttgtc gagcttgtag tgggccgcgg
     2281 ccatggccgc ctcccacacc gagccctcct ggcactcgcc gtcgcccatc accacgaagg
     2341 tgcggtagga agacccgtcg tgcttgccgg ccagggccac gccgttgccc accggcaggc
     2401 cgtgccccag cgagccggtg gcgagctcga cgcccgggat gctcttcagc gagtgcccgg
     2461 cgaggcggct ccccatctgc ttgtacgtct tgagctcctc caccgggaag aagccgcgct
     2521 cggcgagcgc cgcgtagagg gcggcgctcg cgtgcccctt gctgaagatc agatagtccc
     2581 gcccatccat cttgggctgc gccggatcga cccgcagcac gccgtcgaag aacagcacgg
     2641 tcaggatctc ggtaaccgac atcgatccgc cgacgtgagc gccgggcgaa tcggccgcca
     2701 tgtgcacgat gtggtggcgc agggtatgcg cgatgtcctt caggtccgcc cagcggtccg
     2761 cctgggggat ctcggatact ttcgtcaaag ccaagtggtc ttcctcctct tcaacggatc
     2821 gatggggcgg cctcaccaga gccgcgcggc ctgttcccag ggctcgcggt agtcgaggat
     2881 ccgggcgtga tcagtgagat catccagacg cgagaaaacc ttttggaaag cctcgaggta
     2941 gagatcgaca ttgcggctct ccatgaagct cgccatgcac agccggtgtc cgacgagcag
     3001 cgtgctccgg atcacgtcca gcgtctgggg gtattcgtac atgtcgtagg tgacgtcgcg
     3061 gctggtgtgg ccgcaggtcc aggggcaccc ctggccgtag cccttccgct cgacgaacag
     3121 gagctgcccc gggatggggc ggttctggta ctcgatgagc ggcacccctt ctgcctccat
     3181 ggcgtcctgc accgccttgc ggaagacacc gaccggcagg tcgacgcccg ccgccgcggg
     3241 gtccacccgg aaccggaaat ggtggtagac gtgcgtgcga tcgtccggca cgaagggggg
     3301 aatgaggccc gggatctcct ccagtcccct agccaggcgg cgcgcccctt cctgcacccg
     3361 ctccgtgtag gagtcgagcc gctcgagctg gctcagggtg aacgccgcga ggatggagct
     3421 gaaacgatag ttccagccca tggtcaaggc gttgtactcc cgcgcctggt cggggcgtac
     3481 tttctcgccg aacatcttga tcatgtcggc ggcgtcacgc agcgccgggt cgctggtggt
     3541 gaacagcccc ccctcgccgg cggtgggcag gttcttgccg gccatgatgc tgtaggccgc
     3601 catgtcgccg agcccgccgg cctttcttcc cttgtacagg gcgcccggcg cctggcaggt
     3661 gtcttcgatc accttgatgc cgcgccgccg ggcgatctcc aggatcgggt ccatgtcggc
     3721 cggcaaaccg tgcagatgga cgacgaccac ggccttggtc cggtcggtga tccgctcctc
     3781 gatcttcgcg ggatcgagag tgaaggtgcg cggatcgata tcgacgaaga ccggcagccc
     3841 ctggtgatgg atgaccgccg tggccgaggc gaggaaggtc agggcgggga ccagcacttc
     3901 gtcccccggc tgcacgcccg ctgccgccac cgccgcgtgg agcgccgccg tgccgctgtt
     3961 gaaggccagg cagtagcggc taccgatgta gtcggcccac gcctgctcga gcgccggcac
     4021 ggcgtcgttg ttgatccagg agaggcggcc cgagcgcagg gtatcgacaa cggccgcgat
     4081 atcctcctct tcgaccagcg gccagcgctc gacgatggtc cccggtggca cgaccggcgc
     4141 cccgccgtgc agggcgagcc tgccggcgac ggggcgcggc gcgggccgct ccgcttcaat
     4201 caatgacata cagccgcctt tcgacttcgg tgttggtgag gacctcgcgc ccgttctcgg
     4261 tgaccacgac cgtgtcggag aagcccacgt ggccgttc
//
LOCUS       DASPJI010000241         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_188361, whole genome shotgun sequence.
ACCESSION   DASPJI010000241 DASPJI010000000
VERSION     DASPJI010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188361"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="VEL74_06410"
     CDS             <1..1551
                     /locus_tag="VEL74_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HYG62194.1"
                     /translation="QLVRADDVMTRGALTIQADGWRRFQYNPRLDAIAATIPRGTIFD
                     RNGVPLAASNAGDLWKARATYAELGVPLTHMKLENGRRMYPFGGRTFHLLGDLRNRVN
                     WAASNTSYIERDSRIRLQGYDDYAAVVEVRQPDGTMSELVREDFRELIPLLRHRYQLE
                     HPRVKHILERPRDVRTSIDIRLQLAAEQILERYARQVGRGAGAAAVVMDPATGDLLAS
                     AGYPWPGELPVRDAEEAAPALIDRARYGIYPPGSTFKIVTAMAALRKDQGLARQTYEC
                     TALPDGRVGNRVRGWGRPIRDDVTDRVPHGTVDMQKGIRVSCNAYFAQLATYAVQAQA
                     LHETADLLGIKVARPNTPEQLKDALPQAGYGQGQVVATPFQMARVAATVANDGAMPEG
                     RWVTDASNTRTAEPVRILAGMPEGFIARSMREVVTAGTARQLSGVEPAIAGKTGTAEV
                     QGKPSHSWFIGYAPYGAATQAREAQDGKGKRIAFAVIIENGGYGGRAAALAAGEIVRK
                     AAELGLVE"
     gene            1566..2351
                     /locus_tag="VEL74_06415"
     CDS             1566..2351
                     /locus_tag="VEL74_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HYG62195.1"
                     /translation="MDTLGDILKKIRHWVRPWEMGTEPMEVRRAVLEEIEAKVVAAGG
                     GKRIFPYNRVRVHLLVSGPDERAMLEAVVNEGWDLEGEVRRRMRERGVELPPGFGLEV
                     GYTEETGPEFGGRHFHVSYERAEVEPPRPGEPGRPLLELTVTKGQATPQVYSAEADRI
                     YLGRMQEVIDHEGRVRRRNDVAFAEEGEINQTVSREQARVTWDPETRGYWLRAEPGAS
                     ATRIFRDGRAIEVSRQDRRGVRLQAGDEVYLGRACLKVGLRQL"
BASE COUNT          401 a          911 c          963 g          320 t
ORIGIN      
        1 cagctcgtgc gggcggacga cgtgatgacc cgcggcgcgc tgaccatcca ggcggacggc
       61 tggcgccgct tccagtacaa cccgcgcctg gacgccatcg ccgccaccat cccccggggc
      121 accatcttcg accgcaacgg cgtcccgctg gcggcgagca acgcggggga cctgtggaag
      181 gcgcgggcga cctacgccga gctgggggtc cccttgaccc acatgaagct cgagaacggg
      241 cggcgcatgt acccgttcgg cggccgcacg ttccatctcc tgggcgacct gcgcaaccgg
      301 gtgaactggg cggcgtccaa cacctcctac atcgagcgcg actcgcgcat caggctccag
      361 gggtatgacg actacgccgc ggtggtcgag gtgcggcagc cggacggcac gatgtccgag
      421 ctggtcaggg aggacttccg cgagctgatc ccgctgctgc gccaccgcta ccagctggag
      481 cacccgcggg tgaagcacat cttggagcgg ccgcgcgacg tccgcacgtc gatcgacatc
      541 cggctccagc tcgcggcgga gcagatcctg gagcgctacg cccggcaggt ggggcgcgga
      601 gccggcgccg cggcggtggt catggacccg gcgaccggcg atctcctcgc cagcgccggc
      661 tatccctggc cgggggagct gccggtgcgg gacgccgagg aggcggcgcc ggcgctgatc
      721 gaccgggcgc gctacggcat ctatccgccg ggctccacct tcaagatcgt caccgcgatg
      781 gccgcgctgc gtaaggacca ggggctggcg cggcagacct acgagtgcac ggcgctgccc
      841 gacggccggg tgggcaaccg ggtgaggggg tgggggcggc cgatccgcga cgacgtcacc
      901 gaccgcgtgc cgcacggcac ggtcgacatg cagaagggga tccgcgtctc ctgcaacgcc
      961 tacttcgccc agctcgccac ctacgcggtg caggcccagg cgctccacga gaccgccgac
     1021 ctcctgggga tcaaggtggc gcggccgaac acgccggagc agttgaagga cgcgctgccg
     1081 caggcgggct acggccaggg gcaggtggtc gccacgccat tccagatggc gcgggtggcg
     1141 gcgacggtcg ccaacgacgg cgcgatgccc gaggggcgct gggtcaccga cgcgagcaac
     1201 acccgcaccg ccgagccggt gcgcatcctc gccggcatgc ccgagggttt catcgcccgc
     1261 tccatgcgcg aggtggtgac cgcgggcacc gcgcgccagc tctccggcgt ggagccggcc
     1321 atcgccggca agaccggcac cgccgaggtc cagggcaagc cctcccactc ctggttcatc
     1381 ggctacgcgc cctacggcgc cgccacccag gcccgggagg ctcaggacgg gaaagggaag
     1441 cgcatcgcct tcgcggtgat catcgagaac ggcggctacg gcggccgggc ggcggccctc
     1501 gcggcgggcg agatcgtccg caaggcggcc gagctggggc tggtggagta gattaggatc
     1561 aagccatgga caccctggga gacatcctca agaagatccg gcactgggtc cgcccctggg
     1621 agatggggac ggagcccatg gaggtccggc gggccgtgct cgaagagatc gaggccaagg
     1681 tcgtggcggc cggcggcggc aagcggatct tcccgtacaa tcgcgtgcgg gtgcacctgc
     1741 tggtgagcgg ccccgacgag cgcgccatgc tcgaggcggt ggtgaacgag gggtgggacc
     1801 tggaggggga ggtccgccgg cggatgcgcg agcgcggcgt cgagctgccc cccggcttcg
     1861 gcctcgaggt gggctacacc gaggagacgg gtcccgaatt cggcggccgg cacttccatg
     1921 tctcctacga gcgggccgag gtggagccgc ccaggccggg cgagccgggc cggcccctcc
     1981 tcgagctgac ggtgaccaag gggcaggcga cgccgcaggt ctacagcgcc gaggccgacc
     2041 gcatctacct cgggcgcatg caggaggtca tcgaccacga ggggcgggtg cggcggcgca
     2101 acgacgtcgc cttcgccgag gagggggaga tcaaccagac cgtctcccgc gagcaggccc
     2161 gcgtcacctg ggaccccgag acccgcggct actggctgcg cgccgagccc ggggcctcgg
     2221 ccacccgcat cttccgcgac ggccgcgcca tcgaggtcag ccgccaggac cgccgcggcg
     2281 tccgcctcca ggccggcgac gaggtctacc tcggccgcgc ctgcctcaag gtggggttgc
     2341 ggcagctctg acatccgacc tgctccggca tgcgtagcat gcgttaggat gagagcccgg
     2401 attctcatcc cacaggggca ggagagatgg cgaagagcag tgccaccacc gtcgagggat
     2461 cgaggcgagc cggccgaagc aggagaggtc tcatgcggta tccaggtctg gtcctcgccg
     2521 tcgccctgtc tctccccgtg accagtctcg ccgcccacgg ccagaccgtc gatccgctga
     2581 tcctgccgga ggtgg
//
LOCUS       DASPJI010000242         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_415635, whole genome shotgun sequence.
ACCESSION   DASPJI010000242 DASPJI010000000
VERSION     DASPJI010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415635"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VEL74_06420"
     CDS             <1..640
                     /locus_tag="VEL74_06420"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HYG62196.1"
                     /translation="SIDDLPPLLRLGVPVSYAVLPFEEATPTVVAELRRRGVEMLLHL
                     PMEPRNGEDPGPGALRLGMSDEELRQLTVAALRAVPGASGVNNHMGSGLSAHEPSMEA
                     ILGLLATRRLFFLDSRTSAESVGYRVATSLGIPAAERQVFLDDDQTPEAIRTQFQRLL
                     GLARTRGAAIAIGHPHPATLAVLADEVPRARDMGYEFVPVSFLLDRPDEETE"
     gene            complement(795..1121)
                     /locus_tag="VEL74_06425"
     CDS             complement(795..1121)
                     /locus_tag="VEL74_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HYG62197.1"
                     /translation="MNLYVGNLSYRMTEDQLRETFEEFGQVTSCTIIKDKMTGQSKGF
                     GFLEMPERAEAEAAISSLNGRDLQGRKLNVNEARPRTGGGGGGGQGRSNGWGGGGGRD
                     SGRDRY"
     gene            complement(1239..>2178)
                     /locus_tag="VEL74_06430"
     CDS             complement(1239..>2178)
                     /locus_tag="VEL74_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HYG62198.1"
                     /translation="DIYPDHRDGHRGSYWKARALEGLGQPSRARQIYRELVASTDTSD
                     FYGRQATEQLGLVPEESEPVLAQAKTSVWPAEPALRRAKALMDLGLDGLATREAELLG
                     DEASPRDRLALKALMLNRQGRLRESLVLLREAFPALGGPYQSAVPAEILRAYYPLQYE
                     DAIRANAERTGLPPHIVAGIIRQESAFDPRATSPVGARGLMQLMPGTAREVSGKLDMA
                     YDPSRLYDPEVSVRLGTTYFKQLLDGFDGNVELALAGYNGGPNRIRRMWNEASGTGAG
                     LDDFFETLSIEESRNYVKRILVLADSYRQLYPSMRG"
BASE COUNT          295 a          758 c          781 g          344 t
ORIGIN      
        1 cagcatcgat gacctgcctc ccctcctgcg cctgggggtg ccggtctcct acgccgtgct
       61 gccgttcgag gaggcgacgc ccacggtggt ggcggagctg cggcgccgtg gcgtggagat
      121 gctcctccac ctgccgatgg agccgcgcaa cggcgaggac cccggccccg gcgccctccg
      181 cctcggcatg agcgacgagg agctgcgcca gctgaccgtc gccgccctgc gcgcggtccc
      241 cggcgccagc ggcgtcaaca accacatggg ctccggcctc tcggcgcacg agccctccat
      301 ggaggcgatc ctcggcctgc tggccacgcg gcgcctcttc ttcctcgact cgcgcaccag
      361 cgccgagagc gtcggctacc gggtcgcgac ctccctgggc atcccggccg ccgagcgcca
      421 ggtgttcctc gacgacgacc agacgccgga ggcgatccgc acccaattcc agcgcctcct
      481 cggcctggcg cgcacccggg gggccgccat cgccatcggc cacccccacc cggcgacgct
      541 cgccgtcctg gcggacgagg tgccgcgcgc ccgcgacatg gggtacgagt tcgtgcccgt
      601 cagtttcctg ctggaccggc ccgacgaaga gacggaatag ggtgggagag gggtctcgcc
      661 ggtagcgcgg atttacctgc cgccggtctt cctggagaaa aattttccct gacgggcatc
      721 gaggcatggc ctctggcccg tcagggatcg gtcttcagat ctcgtgtgcg atcgggatcg
      781 gagacggaga tcggtcaata gcggtcgcgg cccgagtcgc ggccaccgcc gccgccccag
      841 ccgttcgagc ggccctggcc gccgccgcca ccaccaccgg tccgcggccg ggcttcattg
      901 acgttgagct tgcggccctg taagtcacgg ccgttcaggc tactgatcgc cgcttccgct
      961 tcggcccgct cgggcatctc gaggaacccg aagcccttcg actggccggt catcttgtcc
     1021 ttgatgatgg tgcaagaggt gacctggccg aactcctcga aagtctcccg gagctgatcc
     1081 tcggtcatgc ggtacgacaa gttgcctacg tagaggttca ttctgctgtc tccttggtca
     1141 tgggtctcgg tgtgagtgtc tgcaaaagcc ttgcccaaac caagtaccgg caggaacctt
     1201 cagggcgaag ccgtcgaaaa gggggccgcc gcccagccct acccccgcat ggaggggtag
     1261 agctggcggt agctgtcggc cagcaccagg atgcgcttga cgtagttccg ggactcctcg
     1321 atggagaggg tctcgaagaa gtcgtcgagc ccggcgccgg tgccggaggc ctcgttccac
     1381 atccggcgga tgcggttggg gccgccgttg tagcccgcga gggcgagctc gacgttgccg
     1441 tcgaagccgt cgaggagctg cttgaagtag gtggtgccca ggcggacgct gacctcgggg
     1501 tcgtagagcc gcgacgggtc gtaggccatg tccagcttgc cggagacctc gcgggcggtg
     1561 ccgggcatga gctgcatcag gccgcgggcg cccaccgggc tggtggcgcg ggggtcgaag
     1621 gcgctctcct ggcggatgat gccggcgacg atgtgcggcg gcaggccggt ccgctcggcg
     1681 ttggcgcgga tcgcgtcctc gtactggagc gggtagtagg cgcgcaggat ctccgccggc
     1741 acggcggact ggtaggggcc gccgagggcc gggaaggcct cgcgcagcag caccaggctc
     1801 tcgcgcagcc gcccctggcg gttgagcatc agggccttga gcgccagacg gtcgcgcggg
     1861 ctcgcctcgt cgccgaggag ctcggcctcc cgcgtcgcca ggccatcgag ccccaggtcc
     1921 atcagcgcct tggcgcggcg tagcgccggc tcggccggcc agaccgaggt cttggcctgg
     1981 gcgagcaccg gctcgctctc ctcgggcacc aggccgagct gctccgtcgc ctggcggccg
     2041 tagaagtcgg aggtgtcggt ggaggctacc agctcgcggt agatctgccg tgcccgggag
     2101 ggctggccga ggccctccag ggcccgcgcc ttccagtagc tgccgcggtg gccgtcgcgg
     2161 tggtcggggt agatgtcc
//
LOCUS       DASPJI010000243         6099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_14151, whole genome shotgun sequence.
ACCESSION   DASPJI010000243 DASPJI010000000
VERSION     DASPJI010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14151"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VEL74_06435"
     CDS             <1..835
                     /locus_tag="VEL74_06435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYG62199.1"
                     /translation="STVPESDPERESRLFEDLSRLNNDLATMQREVAKKNVELARLND
                     QKNQFLGMAAHDLRNPLEVILTYSEFLLDEGACLPPEQRELVRTIHGSSAFMLALVND
                     LLDISKIEAGRLELDLSRVDLGELTARNVALNQVLAAKKGTEIVFRQEGELPRMLLDT
                     AKIEQVLNNLIGNAVKFSPPGSTVEVRLAHEGDGGAVLSIADQGEGLPIHEADQMEKL
                     FRPFERTRVRSTGGEKSTGLGLAIVKRIVTGHQGEIRVESEAGKGTTFHVLLPVSRGP
                     G"
     gene            836..2881
                     /locus_tag="VEL74_06440"
     CDS             836..2881
                     /locus_tag="VEL74_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG62200.1"
                     /translation="MEDISKILVVDDDPALLRGYSRILEEAGYAVAQAATGEEALRAC
                     AGEMPDLVLLDSVLPDLDGIEVCRRIKTTKALAGVFIVMLSGQRTSGDQQAAGLEAGA
                     DGYLAKPIERRALLAHIRALLRIKNAEQAIRASEERQRHLVEELTEANRRLEEYNRLK
                     AEFVANMSHELRTPLTAIIGFAQLVKLSEGDTPLPKSCERAFEKILRNARHLLALIDD
                     VLDIAKIEAGRMKIHREHVDLAEVVQDSFGELQSLARQKGLDYRLRVPEELPLAYIDP
                     LRVRQVVINLLSNAIKFTSRGFVELELVPHGNGWCRLVVRDSGVGIEEQAMGIIFERF
                     RQVDGSMSRLAGGAGLGLSIVRQIVELLGGRIEVASKVGEGSVFSVTLPLTGAVAERT
                     VPDASLRLDPSARPGTEPEAPEPEEETASGFENDRRPLVLIVEDDPDTAALLAETVSA
                     AGYGVRMASSGTAGLRLAREILPDVITLDVMMPGMDGWRVLQALKEEPHTAEIPVIVC
                     SVVDNRPLGYRLGASAYLIKPIEPDSLTTTLTGLHTGHEENGEGYVLVVDDEHGVREL
                     LSTALRKAGYRVLAAASGETALRIVAQSLPRALLCDLMMPGGMSGYELIARLRSDPRT
                     EHVPIMVITGKDMTSEDRLFVLGQITDVIRKGDLLMSGIDTRLREALEHIGVNPSHG"
     gene            2874..3269
                     /locus_tag="VEL74_06445"
     CDS             2874..3269
                     /locus_tag="VEL74_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG62201.1"
                     /translation="MARILLIEDEPDTRELVRLTLELDGHDVEEAATAEEGIVQARQQ
                     RPDLILMDLSLAGKFDGLEATKRLRADPAFDQTPIIALTAHAMQSDREKSLAAGCDDH
                     WTKPIVDLALFKSMIGTLAARGRDLRTAR"
     gene            complement(3294..3752)
                     /locus_tag="VEL74_06450"
     CDS             complement(3294..3752)
                     /locus_tag="VEL74_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62202.1"
                     /translation="MALKSLTMATLMAAAILAAGCGTSPAASLSECIMEGAQTLATSR
                     SDTHSMECELKMAGPYKIMSFPPRQLMDGSAYQQAESTLVAAAKTPTLPVPAHESIWI
                     IPGDGQKEEALSYRKSFVPFSQLFSLDKSEPKLTVVLRKTDTGIEVAELR"
     gene            4131..>6099
                     /locus_tag="VEL74_06455"
     CDS             4131..>6099
                     /locus_tag="VEL74_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG62203.1"
                     /translation="MKSSDISGAISLADLTPEQRALLVLRMRRKAAAEAPGIPALRPV
                     PRPASGAMPLSFSQQRLWFMEQWQPGSAAYNIPAALRIRGPLDVAALRWAFDEIVRRH
                     EVLRVRIDPSTPVQAAGPPEPLPVPEVDLAAVPEPWRDREAGRLVHEEVRRPFDLTRD
                     RLLRKVLLRLGETEHVAVFNMHHIVSDGWSVGLLSQEFVTLYMARVQGLPSLFPELPL
                     QYADYAVWQREWLQGEALEAQLEYWRRTLDGAPAETALPLARPRPAVRSGHGARRSYD
                     LRGDLVPALRSLAQREGGTLFMVLLAALDTLLHRHSGETDLVVGTPVAGRGRAELEGL
                     IGFFVNNLALRADLSGDPPFRTLVGRLREVTLAAQAHQDLPFEKLVEELRPERDTSRP
                     PVFQVVLSLQNTPRVRGGLEGLEISPLDFEGGTAAYDLLVSALELETEELTGFFEYST
                     DLFDAVAAARLTGQFQALLAGAAADPGRRLSELPLLSAAERWQLLGEWNDTAVAPAGS
                     KSSGGCFHEIWEERAAAFPDAVGVAFEDQRLSHGELVRRSGLLARHLRTLGVGPETLV
                     GIAMERSIEMVVAILGIWKAGGAYVPLDPSHPEERLDAVIGETGLLVVVTQDRVLPRL
                     PRFFGFTVSIDGGWDAIAGATDDLEVNVQPGN"
BASE COUNT          939 a         2043 c         2148 g          969 t
ORIGIN      
        1 gagcacggtg cccgagtcgg acccggagcg ggagagccgg ctgttcgagg acctctcgcg
       61 gctcaacaac gacctcgcga cgatgcagcg cgaggtggcg aagaagaacg tcgagctcgc
      121 gcgcctcaac gatcagaaga accagttcct gggcatggcg gcgcacgacc tgcgcaatcc
      181 gctggaggtc atcctgacct atagcgagtt cctgctggac gagggcgcct gcctgccgcc
      241 ggagcaacgc gagctggtgc gcacgattca tggctcgagc gccttcatgc tcgccctggt
      301 caacgacttg ctcgacatct cgaagatcga agccggacgg ctggagctgg atctctcccg
      361 ggtggacctg ggagagctga cggcccgcaa cgtggcgctc aaccaggtgc tcgcggcgaa
      421 gaaggggacg gagatcgtct tccggcagga gggcgagctc ccccggatgc tgctcgatac
      481 ggcgaagatc gagcaggtgc tgaacaacct gatcggcaac gcggtcaagt tttcgccgcc
      541 agggagcacg gttgaggtgc gtctggccca tgaaggcgac ggaggggccg tgctctccat
      601 cgcggaccag ggggaggggc tgccgatcca tgaggccgat cagatggaga aactcttcag
      661 acctttcgag cgtacccgag tacggagcac gggcggtgag aagagcaccg gcctggggct
      721 cgcgatcgtc aagcgcattg tcaccgggca tcagggggag atccgggtgg agagcgaggc
      781 cgggaagggt acgaccttcc acgtcctgct gccggtgagc cgggggcccg gctgaatgga
      841 agacatatcg aagatcctgg tggtggatga cgatccggcg ctgctgcgcg gctactcccg
      901 catcctggag gaggcggggt atgccgtggc ccaggcggcg accggagaag aggccctgcg
      961 cgcttgtgcc ggggagatgc cggacctggt cctgctcgac tccgtgctcc cggacctgga
     1021 tggcatcgag gtctgccggc ggatcaagac aaccaaggcg ctggccggcg tcttcatcgt
     1081 catgctctcc ggccagcgga cctcggggga ccagcaggcc gcgggactcg aggcgggcgc
     1141 ggacggctat ctggccaagc cgatcgaacg ccgtgccctg ctggcgcaca tccgcgccct
     1201 gctgcgcatc aagaacgccg agcaggcaat ccgggcgagc gaggagcggc agcggcacct
     1261 ggtggaggag ctgaccgagg ccaaccggcg gctggaggag tacaaccggc tcaaggcgga
     1321 gttcgtggcc aacatgtccc acgagttgcg cacgccgctc accgccatca tcggcttcgc
     1381 ccaactcgtc aagctgagcg agggagacac tccactcccc aagagctgcg agcgggcgtt
     1441 cgagaagatc ctgcgcaacg cccgccacct gctggcgctg atcgatgacg ttctggacat
     1501 cgccaagatc gaggccggcc ggatgaagat tcaccgcgag cacgtggatc tggcggaggt
     1561 ggtgcaggac tctttcggcg agctccagtc tctggcgcgg cagaaggggc tcgattaccg
     1621 gctgcgggtg ccggaggagc tgcccctggc ctacatcgac ccgctccgcg tgcgccaggt
     1681 ggtgatcaac ctgctctcca atgccatcaa gttcaccagc cgcggcttcg tcgagctgga
     1741 gctggtgccg catggcaacg gctggtgccg gctggtggtc cgcgacagcg gcgtcgggat
     1801 tgaagagcaa gccatgggca tcatcttcga gcgcttccgg caggtggacg gatccatgtc
     1861 gcgcctcgcc ggcggcgccg ggctggggct gtcgatcgtg cgccagatcg tggagctgct
     1921 gggcggccgg atcgaggtcg cgtcgaaggt gggggaggga tcggtcttct cggtcacctt
     1981 gccgctgacc ggcgccgtgg ccgagcggac cgtcccggac gccagcctga ggctagatcc
     2041 ctccgcccgg ccgggaacgg agccggaggc gccggaaccg gaagaggaga ccgcttccgg
     2101 gttcgagaac gacaggcgcc ctctggtcct catcgtggag gacgacccgg acaccgcggc
     2161 tctgctggcg gagacggtgt cggccgccgg ttacggcgtg cggatggcga gctcgggcac
     2221 cgccggcctg cgcctcgccc gggagattct gcccgacgtc atcaccctcg acgtgatgat
     2281 gccgggcatg gacggctggc gcgtgctcca ggccctcaag gaggagccgc acacggcgga
     2341 gatcccggtg atcgtctgct ccgtcgtcga caatcggccc ctgggctatc gcctgggcgc
     2401 ctccgcctat ctgatcaagc ccatcgaacc cgactccttg accaccaccc tcaccggcct
     2461 ccacactggc catgaggaga atggcgaagg ctatgtcctg gtggtggacg acgagcacgg
     2521 ggtgcgcgag ctgctgagca ccgcgctgcg caaggccggg taccgggtcc tggccgcggc
     2581 aagcggcgag acggccctgc gcatagtcgc gcagtcgctg ccgcgagccc tgttgtgcga
     2641 cctgatgatg cccggcggca tgagcggcta cgagctgatc gcccgcctgc gttccgatcc
     2701 caggacggag cacgtgccga tcatggtcat caccggcaag gacatgacct ccgaggaccg
     2761 gctcttcgtt ctcggacaga ttaccgatgt gatccggaag ggcgacctcc tgatgtcagg
     2821 gatcgacacc cgcttgcggg aggcgctcga gcatattgga gtcaatccat cccatggcta
     2881 ggatacttct gatcgaggac gagccggata cgcgcgagct ggtcaggctg acgctggagc
     2941 tggacgggca cgacgtggag gaggcggcca ccgccgagga ggggatcgtg caggcgcgcc
     3001 aacaacggcc cgaccttatc ctcatggacc tgagtctggc cggtaagttc gacggcctcg
     3061 aagccaccaa gcgtctgcgc gccgaccccg cgttcgatca gacgccgatc atcgcgctta
     3121 ccgcgcatgc gatgcagtcc gaccgcgaga agtccctggc cgccggctgt gacgaccatt
     3181 ggaccaagcc gatcgtcgac ctggcccttt tcaagtcgat gatcgggacc ctcgcggccc
     3241 ggggcaggga cctgcggacc gcccgctgac gcccgggcct tccggcccgc ctcctaccgc
     3301 agctcggcga cttcgatgcc ggtgtcggtc ttccgcagga cgaccgtgag cttcggctcg
     3361 ctcttatcga gcgagaagag ctgcgagaac ggaacgaagc tcttccggta ggagagcgcc
     3421 tcctccttct ggccgtcgcc gggtatgatc cagatgctct cgtgagccgg gaccggcagg
     3481 gtcggggtct tggcggccgc gaccagggtg ctctccgcct gctggtaggc gctgccgtcc
     3541 atgagctgcc tgggtgggaa gctcatgatc ttgtaggggc ccgccatctt cagctcgcat
     3601 tccatcgagt gggtgtcgga gcgcgacgtg gcgagggtct gggcgccctc catgatgcac
     3661 tcgctcaagc tcgccgcagg actcgtccca cacccggccg ccaggatggc cgccgccatc
     3721 agcgttgcca tcgtcaagga tttaagggcc atgagtgcct ccttttgttg aaaggctggc
     3781 cggcgccgaa agacggcggt agcggacagc catgagctcg atgaggggat gcgggagggg
     3841 ccgacgttcc tcctgcaggg ccgggaggcc ctgtgggctg gcgagccggg aagagcggtc
     3901 ttgtcacggg ccaatacctc ggagcgacga ccaatgatta tggtacgcct gcgcattgaa
     3961 agagtcaaat gaacgccgcg cccgtgggtt gatcccggac agcgatctgt ggcatcgtcg
     4021 gcggcttcgc cggcccgcag gacccctctc cttcgcacac ggctcttccg acgaccagca
     4081 cttccggcag gcccgtcctc ccggttgcgg gcacccatga gggtatggca atgaagtcat
     4141 ctgatatttc cggcgcaatc tcccttgccg atctcactcc cgagcagcgg gcgctgctcg
     4201 tgttgcgcat gcgccgcaag gcggcggccg aagctcccgg catcccggcg ttgcggccgg
     4261 tgccgcggcc cgcgagcggt gccatgcccc tgtcgttctc ccagcagcgc ctctggttca
     4321 tggagcagtg gcagccgggg agcgccgcgt acaacatccc tgccgccctc cgcatccgcg
     4381 gcccgctgga cgtcgccgcc ctgcgttggg cgttcgacga gatcgtccgc cggcacgagg
     4441 tcctgcgcgt ccgcatcgac ccttccactc ccgtccaggc cgctggtccg cccgagcccc
     4501 tgcccgtccc ggaggtggac cttgccgccg tgccggagcc gtggcgggac cgggaggccg
     4561 ggcggctcgt ccacgaggag gtccgccgtc cgtttgacct cacgcgcgac cggttgctgc
     4621 gcaaggtgct gctgcgcctc ggggagaccg agcacgtggc ggtcttcaac atgcaccaca
     4681 tcgtgtcgga cggctggtcg gtgggcctcc tctcccagga gttcgtcacc ctctacatgg
     4741 cgcgggtcca ggggctcccc tcgctgttcc ccgagctgcc gctgcagtac gccgactacg
     4801 ccgtctggca gcgcgagtgg ctccagggcg aagccctcga ggcgcagctc gagtactggc
     4861 ggcggaccct cgacggcgct cccgcggaga cggccctccc cctggcccgg ccgcggccgg
     4921 cggtgcggag cgggcacggc gcccgccgct cctatgacct ccggggagat ctcgtgcctg
     4981 ccctccggtc gctggcgcag cgcgaggggg gcaccctctt catggtcctc ctcgccgccc
     5041 tcgacaccct gttgcaccgc cactccggcg agacggatct ggtggtgggg acgccggtgg
     5101 cgggccgcgg ccgtgccgag ctcgaagggc tgatcggctt tttcgtcaac aacctcgccc
     5161 tgcgcgccga cctctcgggc gatcccccct tccggaccct ggtcggccgc ctgcgcgaag
     5221 tgacgctcgc cgcccaggcg caccaggacc tgcccttcga gaaactggtc gaggagctga
     5281 ggcccgagcg ggacaccagc cggccgccgg tgttccaggt ggtcctctcg ctgcagaaca
     5341 cgccgcgggt gcgaggtggc ctggaggggc tcgagatctc gccgctcgat ttcgaggggg
     5401 gcacggccgc ctatgacctc ctggtctcgg ccctggagct ggagacggag gagctcaccg
     5461 gcttcttcga gtacagcacc gatctcttcg acgccgtggc cgctgcgcgg ctcaccggcc
     5521 agttccaggc gctgctcgcg ggcgcggcgg ccgaccccgg ccggcgcctg tcggagctgc
     5581 ctctcctctc cgcggcggag cgctggcagc tcctggggga gtggaacgac acggccgtcg
     5641 ccccggccgg ctcgaaatcc agtggcggct gtttccacga gatctgggag gagcgcgccg
     5701 ccgctttccc cgacgccgtg ggcgtcgcct tcgaggacca gcggctgagc catggcgagc
     5761 tcgtccggcg gtccggcctc ctggcgcgcc acctgcggac gttgggtgtc ggcccggaga
     5821 cgctggtggg gatcgccatg gagcggtcca tcgaaatggt tgtcgccatc ctgggaatct
     5881 ggaaggcagg gggcgcctac gtgcccctgg acccctcgca tccggaggag cgcctggacg
     5941 cggtgatcgg cgagacgggg ctcctggtgg tcgtgacgca ggaccgggtg ctgccgcggc
     6001 taccgcgctt cttcggcttc acggtctcga tcgacggcgg gtgggatgcg atcgccggcg
     6061 cgacggacga tctggaagtg aacgtccagc cgggcaacc
//
LOCUS       DASPJI010000244         3929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_188561, whole genome shotgun sequence.
ACCESSION   DASPJI010000244 DASPJI010000000
VERSION     DASPJI010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188561"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VEL74_06460"
     CDS             <1..613
                     /locus_tag="VEL74_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYG62204.1"
                     /translation="LASLLVGLYQPTEGRILFDGNDLSELDMRSVRRQVGIVPQHPYL
                     FGASIRSNILLSDPTLPLARAIEAAERAHIHQDILEMPMGYETLLADGGASLSGGQRQ
                     RLALARALVHRPAILLLDEATSSLDTVTEARIQKELETLRSTRIVIAHRLSTIRGADL
                     ILVMDEGRIVEQGRHEELLARGGIYAGLVAAQMEKEKVRTAGL"
     gene            688..2403
                     /locus_tag="VEL74_06465"
     CDS             688..2403
                     /locus_tag="VEL74_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic peptide export ABC transporter"
                     /protein_id="HYG62205.1"
                     /translation="MRPIIQLFSFLLRISKDIPRSRATLAFVTFAGIVSGVASTAMIG
                     VITASVARQRPVEELAWVFLALVILLPIFRFFSQVLLINLNQRSLRTLRVRLVRRILA
                     APLRHLEKTGPSRLLATMTQDVGTVVEALSGLPGLFMHLAIVVSALTYLGMVSLPVLF
                     YITAFIVIGIVTYQLPIIKAMGYMQRSRQLFDNMTGYIRAAVEGTKELKMHRNRRDAF
                     IGAFDQSVEEFQQQGKTGSVIFVAAASWGHVLFFVVIGLLVFVVPRFQPVPPDVLAQY
                     TIILFFMMTPLEMLMNTLPMLGRAAVAVKAVEDLGFTLDNQVKERELPSGETVSPQWR
                     SMELAGVCHSYHRENEEESFQLGPINLAFQPGEAVFLVGGNGSGKTTLAKLLLGLYAP
                     ESGEIRLNGQAITDENREWYREHFSAVFVDFFIFEKLLGIEASSLDDNARKYLRQLHL
                     EQKVQVKGGELSTVELSQGQRKRLALLTAYLEDRPIYLFDEWAADQDPFFKEIFYLEL
                     LPELKQRGKTVFVISHDDRYYYVADRIIKLDYGKVVSDRSMADFLDSMTTENGSLRQS
                     GAAGI"
     gene            2413..3045
                     /locus_tag="VEL74_06470"
     CDS             2413..3045
                     /locus_tag="VEL74_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018070847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYG62206.1"
                     /translation="MDPWALAALAGLSAGLVHALSGPDHVAAVAPLALAERRSRWRTG
                     LLWGIGHSAGVCLVGLLALLLREVLPLDSLSSWSERLVGAVLIVVGLWGLRRASLARL
                     PVPHQHAEEEPGAPRSRQAALWIGALHGLAGSSHLLGLFPALALPSRAASLAYVGGFS
                     VGTVAAMVVFSSTIGLLAAWLTARGQNAYRALLGTASAAAIGVGIFWLTL"
     gene            3520..>3929
                     /locus_tag="VEL74_06475"
     CDS             3520..>3929
                     /locus_tag="VEL74_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62207.1"
                     /translation="MRSTFSGTRFAGWYFAVQGVAIAGWWLYLSLRPGAVRLFVPPGA
                     QDIDLIAFRLPDLLVAVPASLIAAVTFFARSRWAVPLGWLAAGAMDYAFFYCLAWSWH
                     RQGGWLSVVLMAPAALLSTVGALDTSAEHLTLFRR"
BASE COUNT          544 a         1371 c         1325 g          689 t
ORIGIN      
        1 gctggcgagc ctgctggtcg gcctctacca gccgaccgag gggcggatcc tgttcgacgg
       61 caacgacctc tccgagctgg acatgcgctc ggtgcgccgg caggtgggga tcgtccccca
      121 gcacccgtac ctgttcggcg ccagcatccg ctccaacatc ctgctgtcgg accccaccct
      181 gccgctggcc cgtgccatcg aggcggcgga gcgggcgcac atccaccagg acatcctgga
      241 gatgcccatg ggctacgaga ccctgctggc cgacggcggc gcgtcgctgt cgggcggcca
      301 gcgccagcgc ctcgccctgg cccgcgccct ggtccaccgc ccggcgatcc tgctgctcga
      361 cgaggccacc agctccctcg acacggtgac cgaggctcgg atccagaagg agctcgagac
      421 cctgcgctcg acgcgcatcg tcatcgccca ccggctgagc accatccgcg gggccgacct
      481 catcctggtc atggacgagg gacgcatcgt ggagcagggg cggcacgagg agctgctggc
      541 gcggggcggc atctacgccg gcctggtcgc cgcccagatg gagaaggaga aggtgcggac
      601 cgccggcctc tgagccgcct gcgggaccgg atgtacggcg gatcggctag aatcggctct
      661 gttttctatc ggctcgagga gtagaccatg cggcccatca tacagttgtt ttcttttctg
      721 ctgcggatct ccaaagacat cccacgctcg cgggccacgc tggcgttcgt gaccttcgcc
      781 ggcatcgtga gcggcgtggc gagcacggcg atgatcggcg tcatcaccgc ctcggtggcc
      841 aggcagcggc ctgtcgagga gctggcgtgg gtcttcctgg ctctggtcat cctcctgccg
      901 attttccggt ttttttcaca ggtgctgctg atcaacctga accagcgcag cctccggacg
      961 ttgcgggtgc ggctcgtccg ccgcatcctg gcggcaccgc tgcgccacct ggagaagacc
     1021 gggccgtcac gcctgctggc gaccatgacc caggacgtgg gtacggtcgt ggaggccctg
     1081 tccggcctgc cgggcctgtt catgcacctg gcgatcgtgg tgagcgccct gacctatctc
     1141 ggcatggtgt cgttgccggt gctgttctac atcacggcct tcatcgtcat cggcatcgtg
     1201 acctaccagc tcccgatcat caaggcgatg ggctacatgc agcgcagccg ccagctgttc
     1261 gacaacatga ccggctacat ccgggccgcg gtggagggca ccaaagagct caagatgcac
     1321 cgcaaccggc gcgacgcctt catcggcgcg ttcgaccaga gcgtcgagga gttccagcag
     1381 cagggcaaga ccggttcggt catcttcgtc gcggcggcga gttggggtca tgtgctcttc
     1441 ttcgtcgtca tcgggctcct ggtcttcgtg gtgccgcgct tccagccggt gccgccggac
     1501 gtgctggccc agtacacgat catcctcttc ttcatgatga cgccgctcga gatgctgatg
     1561 aataccctgc ccatgctcgg ccgggccgcg gtggcagtca aggcggtcga ggacctcggg
     1621 ttcactctgg acaatcaggt gaaggagcgg gagctgccgt ccggggagac ggtgtcgccg
     1681 caatggcgca gcatggagtt ggcgggcgtc tgccacagct atcaccgcga gaacgaggag
     1741 gagagcttcc agctcggtcc catcaacctc gccttccagc ccggcgaagc ggtgttcctc
     1801 gtcggcggca acggcagcgg caagacgacg ttggccaagc tgctgctggg cctctacgcg
     1861 ccggagtccg gcgagatccg cttgaacggc caggccatca cggacgagaa ccgggagtgg
     1921 taccgcgagc acttctcggc ggtcttcgtc gacttcttca tcttcgagaa gctgctcggc
     1981 atcgaggcct cgtcgctgga cgacaacgcc cgcaagtacc tgcggcagct ccacctcgag
     2041 cagaaggtgc aggtcaaggg cggcgagctg tcgaccgtgg agctctccca gggccagcgc
     2101 aagcgcctgg cgctgctgac cgcctacctg gaggaccgcc cgatctatct cttcgacgag
     2161 tgggcggcgg accaggaccc gttcttcaag gagatcttct atctggagct cctgcccgag
     2221 ctcaagcagc gcggcaagac ggtgttcgtc atcagccacg acgaccgcta ctactacgtg
     2281 gccgaccgca tcatcaagct ggactacggc aaggtggtct ccgaccgctc gatggccgat
     2341 ttcctcgact cgatgaccac cgagaacggc tcgctgcggc agagcggggc cgcgggaatc
     2401 tagggcgtgg ccatggatcc ctgggctctc gccgccctgg ccggcctgtc ggcggggctg
     2461 gtccacgcgc tctccggccc cgaccacgtg gcggcggtgg cgccactcgc cctggccgag
     2521 cggcggtccc gctggcggac gggcctgctc tgggggatcg gccacagcgc cggcgtctgc
     2581 ctggtgggcc tgctcgccct gctgctgcgc gaggtgctgc cgctggacag cctctcctcc
     2641 tggagcgagc gtttggtagg cgcggtgctc atcgtggtgg ggctatgggg actgcgccgg
     2701 gcctcgctcg cgcggctgcc ggtcccgcac cagcacgcgg aggaggagcc cggcgcgccg
     2761 cgctcccgtc aggcggcgct ctggatcggc gccctgcacg gtttggccgg cagctcccac
     2821 ctcctcggcc tcttcccggc gctggcgttg ccttcgaggg cggcatcgct cgcctacgtc
     2881 ggcggcttca gcgtcggcac cgtggcggcg atggtcgtct tctcctcgac catcggcctg
     2941 ctcgccgcct ggctgacggc gcgcggccag aacgcctacc gcgccctcct cggcacggcc
     3001 tcggccgccg ccatcggcgt cggcatcttc tggctgacgc tctgaggctt gccccgcggg
     3061 tcgagtcgtc cgccctgtgg cgaaggccgg cctcgacgcc cggacggtca gggctactcc
     3121 ccgtaggaaa aggaggggtc ccgccgcagc ggacggagcc cggctgctcc ggattcttcc
     3181 cgagggctcc ggatacctcc cggacgccgc ggatcgagtc ctgttcaaaa atatggagtc
     3241 tggacgccgc ggaatcttcc cgagcggttc ggagcgcgtc cggacgcggc ggatggagtc
     3301 cgattcaacc ggatgaattc cgcggacgcc gggaagcttc ccgggcctcc cggatcgcgt
     3361 ccggttcaac cggatggagc ccgcacggcc cggacgaggt cgcgggccgg agagctcgac
     3421 gacccgctga cgcccggagc tcccgctcct gtagactgat ccggccgaga ccggtgtcag
     3481 atcggctcta tggagctcac gggtaggcgc cctggatcag tgagatcgac cttttccgga
     3541 acccggttcg ccggctggta tttcgctgtg caaggggtgg ccatcgccgg ctggtggctc
     3601 tacctgagcc tgcgtcccgg cgccgtccgg ctgttcgtgc cgccgggcgc gcaggacatc
     3661 gacctgatcg cctttcgcct cccggacctg ctggtcgccg tccccgcttc gctgatcgcg
     3721 gccgtcacgt tcttcgcccg gtcgcggtgg gctgtccctc tcggctggct tgccgcgggc
     3781 gcgatggact acgccttctt ctattgcctc gcctggtcct ggcatcggca ggggggctgg
     3841 ctcagcgtgg tgctcatggc gccggcagcc cttctctcca ccgtcggcgc gctcgacacc
     3901 agcgccgagc atctcaccct cttccgtcg
//
LOCUS       DASPJI010000245         3988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_308203, whole genome shotgun sequence.
ACCESSION   DASPJI010000245 DASPJI010000000
VERSION     DASPJI010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308203"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1872
                     /locus_tag="VEL74_06480"
     CDS             <1..1872
                     /locus_tag="VEL74_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG62208.1"
                     /translation="VPAGEKPEAGELRRFLSQSLPAWMVPAAWVLLDALPLSPAGKVD
                     RRALARIDPEPEARATVQDAPRSPAEEALARIWAEVLRVERVGVNDDFFELGGDSILS
                     IQVVARAAEAGLQVTPRQVFEHPTVAALAAVAVATGGEAGTVAVEGPAPLTPIQRVFF
                     AREPADLHRFNMSVLLVPREPLDVASLEHALGILLDRHDALRLRFAKRNGEWTQRVDA
                     PGGPVPLTRLDLSALSPALSDAEVEAVFAAAADALQGSLDLERGVVRAALIAAAGRER
                     LLLVIHHLAVDAVSWRVLLEDFERSYLGGENTRLPPATTPFSRWAVTLETYAGSAEVA
                     RELPFWRAQADAAVSRPPLPLDGTGGDNGEDTEATSRAVTVSLDPAMTRAFLKDAHRA
                     YQTRPEELLLAALVEAVGGWTGERALQVDVEAHGREPVFAGAEGIDLSRTVGWFTSVH
                     PVWLDLRSAQGPGGAVKTTKEALRRVPYRGLGYGVLRHLRGEAFAAPPPEIAFNYLGQ
                     IDLLLGGSALFDVDPEPPGLPRSPRAARPYRFEVNAWVRDGRLTVSWGHGERRHRRDT
                     VERLAERFLAVLRDLIDHCLAPDAGGFTPSDFPAAALDQKSLDRLLGNLQSRGGRRR"
     gene            1869..>3988
                     /locus_tag="VEL74_06485"
     CDS             1869..>3988
                     /locus_tag="VEL74_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG62209.1"
                     /translation="MIQDVYRLSPLQEGMLFESLYAGGDAYVVQVSWTLAGDLDAAAL
                     RRAWEWLTVRHAVLRTSFHWEEMEHPHQVVHRQVELPWDEEDWRGVPAGERQGRLEER
                     QQGECRAGFDLEAAPLFRLLLVRLEDRLWSLTWTQHHLILDGWSVGLVLPELFAAYAA
                     FLEGKDPASPPARPFRDYIEWLLRRDPHAAEAFWHHELAGFAAPTPVGGRPAEPEERH
                     AFGGRGRAVSVLDAALTTALSELARRHRLTLNTFVQGAWALLLARRSGEDDVVFGVVV
                     SGRPAAELPGVESAIGPFINTIPLRARLAPGDRLLPWLAALQQRQVEARRFEHSPLVD
                     VQGWTDVPRGRPLFESLMAFEGSLYDPALGGGAGGVRVLDARSNQVSRAPLTFTAVPE
                     GALFYLDLSYNRDRFASDEAQRWVGHLLTLLQSMIANPERVLADLPFLTEPERHQVIS
                     EWNDTGEGPSYPETLHGSFLAQAERAPDALALVFEDERITYGHLAARSGRLAARLRRL
                     GVGPEVRVAVSLPRSPRLVETLLAVLQAGGAYVPLDPNDPPERREWIAADCGATLVIT
                     EEGEERMTAGRGEGRGEADAESLAYVIYTSGTTGRPKGAMVTHAAAVNNLRWAQAIYD
                     LRPGDAGLFKTPLGFDVSVRELLWPLTAGARVVIARPGGERDPGYLLDLLDREGVSVA
                     SFVPSLLAAVLDRETLPTLPTLRRV"
BASE COUNT          501 a         1431 c         1452 g          604 t
ORIGIN      
        1 gtgcccgcgg gggagaaacc cgaagcgggg gagctgcgcc gcttcctttc ccagtcgctc
       61 cccgcctgga tggttccggc cgcgtgggtc ctcctcgacg cgctgccgct gtcacccgcc
      121 ggcaaggtcg accgccgggc cctggcgcgg atcgaccccg agccggaggc cagagcgacg
      181 gtgcaggacg cgccgcgcag cccggcggag gaggccctcg cccgcatctg ggcggaggtg
      241 ctgcgggtcg aacgcgtcgg ggtgaacgac gacttcttcg agcttggggg cgactcaatc
      301 ctgagcatcc aggtggtcgc gcgcgcggcc gaagccggcc ttcaggtgac gccgcggcag
      361 gtcttcgagc accctacggt ggcggctctg gcggcggttg cggtggcgac cggtggagaa
      421 gccggcacag tggctgtcga aggtcctgcg ccgctcaccc cgattcagcg cgtcttcttc
      481 gcgcgcgagc cggccgacct ccaccgcttc aacatgtcgg tcctcctcgt tccgcgcgag
      541 ccgctcgatg tcgccagcct ggagcacgcg cttggcatcc tcctcgaccg ccacgacgcc
      601 ctgcggctgc gcttcgcgaa gcggaacggg gagtggacgc agcgggtgga cgcgcccgga
      661 gggccggtgc cgctgacgcg cctggacctg tccgcgctgt cccccgcgct gtctgatgcg
      721 gaggtggagg cggtcttcgc ggcggcggcg gacgccctcc aggggagcct cgacctggag
      781 cgaggcgtgg tccgcgccgc cctcatcgcc gccgcgggga gggagcggct gctgctggtc
      841 atccatcacc tggcggtgga cgccgtctcc tggcgggtgc tgctggagga ctttgagcgc
      901 tcctatctcg gcggtgagaa cacgcgtctg cccccggcga ccacgccgtt ctcgcgctgg
      961 gcggtgacgt tggagacgta cgcgggatcg gccgaggtgg cgcgggagct cccgttctgg
     1021 cgtgcccagg cggacgccgc ggtcagccgg ccgcccctcc cgctcgacgg caccggcggc
     1081 gacaacggcg aagacacgga ggccaccagc cgggcggtca ccgtctccct cgacccggcg
     1141 atgactcgcg cgttcctgaa ggatgcgcac cgcgcctacc agacacgtcc cgaggagctg
     1201 ctcctggcgg cgctcgtcga agcggtgggc ggttggaccg gcgagcgggc gctccaggtg
     1261 gacgtcgaag cgcacggccg tgagccggtc ttcgcggggg cggaggggat cgacctgtcg
     1321 cggaccgtcg gctggttcac cagcgtccac ccggtctggc tcgacctgcg gagcgcgcag
     1381 gggccggggg gggcggtcaa gacgaccaaa gaggccttgc ggcgcgtgcc ctaccgcggg
     1441 ctcggctacg gcgtgctgcg ccacctgcgc ggcgaagcct tcgccgcgcc gccgcccgag
     1501 atcgccttca actacttggg ccagatcgac ctgctcctag gcggctcggc tctcttcgac
     1561 gtcgaccccg agccgcccgg cctgccccgc agcccgcgcg cggcgcgtcc ttaccggttc
     1621 gaggtcaacg cctgggtgcg cgacggccgg ttgaccgtga gctggggcca tggcgaacgg
     1681 cggcaccggc gcgacacggt cgagcgcttg gcggagcggt tcctcgcggt cctgcgcgac
     1741 ctcatcgatc actgcctggc gccggacgcg ggcggcttca ccccctccga ctttccggcc
     1801 gcggccctgg atcagaagtc gctcgaccgc ctgctcggca acctccagtc gcgcggaggc
     1861 cggcgccgtt gatccaggac gtctatcgcc tctcgccgct ccaggagggg atgctcttcg
     1921 agagcctcta cgccggaggg gacgcttacg tggtccaggt ttcctggacg ctggcgggcg
     1981 atctcgatgc cgcggcgctt cggcgggcct gggaatggct gaccgtgcgg cacgcggtgc
     2041 tccgtacatc gttccactgg gaggagatgg agcacccgca ccaggtagtg caccggcagg
     2101 tggagttgcc ctgggacgag gaggactggc gcggggtccc cgccggcgag cggcaggggc
     2161 gtctggagga gcgccagcag ggcgagtgcc gggccggctt cgacctggag gccgcgccgc
     2221 tcttccggct cctcctcgtt cgtctggagg accggctctg gagcctgacg tggacccagc
     2281 accacctgat cctcgacggc tggtcggtcg gcctggtgct gccggagctc ttcgcggcct
     2341 atgcggcctt cctggaggga aaggaccccg cgtcgcctcc ggctcggccg ttccgcgact
     2401 acatcgagtg gctgctgcgg cgcgaccctc acgcggccga ggcgttctgg catcatgagc
     2461 tggcgggttt cgccgctccg acgccggtgg ggggcagacc cgcggaaccg gaggagcggc
     2521 acgccttcgg cgggcgcggc cgggccgtct cggtgctcga cgccgcactc acgaccgcct
     2581 tgagcgaatt ggcgcggcgc caccggttga cgctcaacac cttcgtccag ggggcctggg
     2641 cgctgctcct ggcacgccgt tccggcgagg acgacgtggt cttcggcgtg gtggtctccg
     2701 gccggccggc ggccgagctg ccgggcgtcg agtcggcgat cggcccattc atcaacacga
     2761 tcccgctgcg cgcgcgcctg gcgccaggcg atcggctgct cccatggctg gccgcgctcc
     2821 agcagcgcca agtcgaggcc cggcggttcg agcacagccc gctggtggat gtccagggct
     2881 ggaccgacgt gccgcgcggc cggccgctct tcgagagcct catggccttc gaaggctcgc
     2941 tgtacgaccc cgccctgggt gggggagcgg gcggcgtacg cgtcctggac gcccgctcca
     3001 accaggtcag ccgcgcgccg ctcacgttca ccgcggtacc cgaaggcgcg ctgttctacc
     3061 tcgacctctc ctacaaccgc gaccgcttcg catccgatga ggcccagcgc tgggtcggcc
     3121 atctcctgac tctgctccag agcatgatcg ccaaccctga gcgggtgctc gcggatctcc
     3181 cgttcctgac cgagccggag cggcaccaag tcatctccga atggaacgac accggcgagg
     3241 ggccttcgta cccggagacg ctccacggtt cgttcctggc gcaggcggag agggcccccg
     3301 atgccctggc cctggtcttc gaggacgagc ggattaccta cggtcatctc gcggcgaggt
     3361 ccggccgctt ggcagcgcgc ctgcggaggc tgggcgtggg gccggaggtc cgggtcgccg
     3421 tctccctgcc gcgctcgccg cggctggttg agacccttct cgccgttctc caggccggag
     3481 gcgcctacgt ccccctggac ccaaacgatc cgccggagcg gcgggagtgg atcgcggcgg
     3541 actgcggcgc cacgctggtc atcaccgagg agggggagga gcggatgact gcaggcaggg
     3601 gggaggggag aggagaggcg gacgcggagt ccctcgccta cgtgatctac acctccggca
     3661 ccaccggccg ccccaaaggc gccatggtga cgcacgccgc cgcggtcaac aatctgcgct
     3721 gggcacaggc catctacgac ctccgtcccg gcgatgcggg cctcttcaag acccctctcg
     3781 gtttcgacgt ctccgtccgc gagttgctct ggccactcac ggctggcgcc cgggtggtga
     3841 tcgcccggcc gggcggggag cgcgatcccg gatacctgct cgacctgctc gaccgggaag
     3901 gcgtttccgt cgcgagcttc gttccctcgc tactcgccgc cgtcctcgac cgggagaccc
     3961 tgcccacact tccaacattg cggcgggt
//
LOCUS       DASPJI010000246         6461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_14417, whole genome shotgun sequence.
ACCESSION   DASPJI010000246 DASPJI010000000
VERSION     DASPJI010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14417"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3576
                     /locus_tag="VEL74_06490"
     CDS             <1..3576
                     /locus_tag="VEL74_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62210.1"
                     /translation="DRRALLALGGPELAPRAEHVAPRTPVEESLAAIWAEVLKLPQVG
                     VYDNFFEIGGQSLLATQVISRVREVFRVELPVRVLFQRPEVAGLAEAVEEAALAGRGI
                     APAPPIVPVPRGGELPLSFSQERLWFIDRLRPGITAYNIFGAVRMRGDLDVAVLESCF
                     DELARRHEVLRTTFTEINGRPSQIINPPSSIRVPLVDLTALPEEGRHALAQRLGNEEA
                     QRPFDLVRGPLLRGVLLRIGGRDHLLALTAHHIVYDVWSRELLVRELGTLYEAFWHGR
                     PSPLPVLPVQYADFASWQRSWMQGDVLAAQLNYWKEKLAGLSGGSELPGDRPRPAVQS
                     FRGARALLELPAEVTAALSGVARKSGATLFMALLAGFYGSLRRMTGDDDVAVGTPIAN
                     RNRAETEGLIGFFVNTLVFRASAAGDPTFRELLARTRETALGGYSHQDVAFEQLVNEL
                     KPRRDTSRQPMFQILFNFLTNYRPVVMELPGLVLSPEPNHSGATQFDLILSMYELDGS
                     LHFSADYSSDLYDAPTMARMLGQYANVLADAARRPDVRLSRIALVGEPERHQLLREWD
                     DTEHRFSSQGLCIHHLFAAQAAATPEAPAVSAGGTPWTYAELDRRSSALAASLRRLGV
                     GPETRVGLYLERSPEIVAALLAVLKAGGAYVPLDPSYPEDRLRFMLEHSGARVVVTQP
                     SLSGRLGGAVQELTLGPGTWDASPIEGEEPYPDPEHLAYLIYTSGSTGRPKGVMVNHR
                     TLVASTLARMAYYKEVPRSFLLLSSFAFDSSVAGIFGTLCTGGKLVVSPPGLAENPER
                     LTELIRHEEVSDFLCVPSLYSHLVERPELADIRGVVVAGEACPADLVDLHFRLHPRAG
                     LWNEYGPTEGTVWSSVYAFRPWDRLERVPIGRPIAGVLLYVLDEAGEVAPLGVPGELF
                     VGGACVARGYEGRPELTAEKFVPDPFGPEPGGRLYRTGDLVRYLPGGDLLFLGRIDQQ
                     VKVRGYRIELEEIEAVLRQHQALREAVVVPLEARKKGAEAADTGAPQYQRLAAYVVAR
                     QGEPAPQAAALRAFLKERLPEYMIPHGIVVLPELPLSPNGKVDRRALAAAGGEAFAPT
                     VPYVAPRNAMEGRLVEIWKELLEVDRVGVQDSFFDLGGHSLLTTRLISRVRDAFQVEV
                     PLQTFFEEPTVAGLGQSIELARWAEEVAREAPATVGAEGYEEGEI"
     gene            3717..6179
                     /locus_tag="VEL74_06495"
     CDS             3717..6179
                     /locus_tag="VEL74_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG62211.1"
                     /translation="MDRPAGGHDGERFLLRLCNPGEYAPEEVGSELAWLLRLRHDEGL
                     LVPEPVRTRDGGLLTRAAVDEAAEAAAPWCCALFRWVPGQVLQGNLTPEAVGGVGTLL
                     ARLHRAAERFEPPPGFARPRWDCDRLFGTAAEGEVVPRDMDDPLVSRRAREILAEAAA
                     VVRQTAEGLGQGPGVFGMIHKDLEPDNIVISDGAPCAIDFGDCGWGWFLYDVAASLLP
                     LREKKGFAEMRDAFLAGYRSVRPLTSEEEELVETFTIARSLFAIRLMLRETMSIPKVR
                     EYAQVAIPHMLGEIRRFLERRGGFAGGGESVDEAGRMTTLQFLGRLRELGIKVWAEGE
                     RLRFSAPQGALTPELKAELAERKQEVLAFLDQGSRAAGPGPMPPEVAASGRLPLSFAQ
                     ERLWDRHRLEPRSAAGHVARAVRLAGPVRPDLLERGLRQAVRRHSILRTTFGEAEGRP
                     YQTVAAVTAILPVVDLGSLSAEARQPEAERLAAELAHRPFDLERGPLLRLVLLRLEPR
                     EHVAVAVLHGLVGDDWSAGLLFREIGALYEAYATERAPLLPEPPLQYADFAWWQREHL
                     QGETLEKPLAFWRQELAGAPARAALPFDRPSARIEGRGERHASLLPPEVIEPLRELGE
                     SQGGATLSMVLLAAFAALLARVSGQDDLVVGSPFAHRGRPELDGLIGPFVNALALRVR
                     LAGDPTFRELVGRVRQTVLAAQAHQELPIEALRDGSVHHPPFSVTFSLQNVPAPDLQV
                     ATFRMTLLPPIARVAQVDLAVDMKDMPEGVRVSWRYRPELFEAATVARLAEAYLALLK
                     DVGRCPDRLSSVLLPAEVTAAS"
     gene            6176..>6461
                     /locus_tag="VEL74_06500"
     CDS             6176..>6461
                     /locus_tag="VEL74_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62212.1"
                     /translation="MSSTPLAQPLAHHDEVDRRLLADFQEFHARLPSRDKVIYMFFTT
                     DLLHWLSRALKFVPEDVNLVLIGSGLLPEEIAWIERHARRPFHHIASRVDD"
BASE COUNT          803 a         2351 c         2344 g          963 t
ORIGIN      
        1 gaccggcggg cgctgctggc gctgggcggt ccggagctgg cgccgcgggc ggagcacgtg
       61 gcgccgcgga cgccggtgga ggagtcgctg gcggcgatct gggccgaggt tctgaagctc
      121 ccccaggtcg gcgtgtacga caacttcttc gagatcggcg gccagtcgct cctggcgacg
      181 caggtcatct cccgcgtccg cgaggtgttc cgcgtcgagc tgccggtgcg ggtgctgttc
      241 cagcggccgg aggtggcggg gctggcggag gcggtggagg aggccgcgct ggccggccgg
      301 gggatcgcgc cggcgccgcc gatcgtgccg gtgccgcgcg gcggcgagct gccgctgtcg
      361 ttctcgcagg agcggctctg gttcatcgac cggctgcggc ccgggatcac ggcctacaac
      421 atcttcggcg cagtgcgcat gcggggtgac ctggacgtgg cggtcctgga gagctgcttc
      481 gacgagctgg cccggcggca cgaggtgctc cgcaccacct tcaccgagat caacggccgg
      541 ccgtcccaga tcatcaaccc gccgtcgagc atccgcgtcc cgctcgtgga cctcaccgcg
      601 ctgccggagg aggggcggca cgccctcgcc cagcgcctgg gcaacgagga ggcccagcgg
      661 ccgttcgacc tggtgcgggg gccgctgctg cgcggcgtcc tgctgcgcat cggcgggcgc
      721 gaccacctgc tggcgctcac ggcgcaccac atcgtctacg acgtctggtc gcgcgagctg
      781 ctggtgcgcg agctgggcac gctctacgag gcgttctggc acggccggcc ctcccccctg
      841 cccgttctgc cggtgcagta cgccgacttc gcgtcctggc agcggagctg gatgcagggg
      901 gacgtcctgg cggcgcagct caactactgg aaggagaagc tcgccggcct ctccgggggc
      961 agcgagctgc cgggcgaccg gccgcggccg gcggtgcaga gcttccgcgg cgcccgcgcc
     1021 ctgctggagc tgccggcgga ggttactgcc gcgctctcgg gcgtcgcccg gaaaagcggc
     1081 gccaccctgt tcatggcgct cctcgccggc ttctatggct ccctgcgccg gatgaccggg
     1141 gacgacgatg tggcggtggg cacgccgatc gcgaaccgca accgggcgga gaccgaaggg
     1201 ctgatcggct tcttcgtcaa caccctggtg ttccgggcga gcgcggcggg cgatcccacc
     1261 ttccgcgagc tgctggcgcg cacgcgggag acggcgctgg gggggtactc ccaccaggac
     1321 gtcgccttcg agcaactcgt caacgagctc aagccgcggc gggacacgtc ccgccagccg
     1381 atgttccaga tcctgttcaa cttcctgacc aactaccggc cggtggtcat ggagctgccg
     1441 ggcctggtgc tgtcgccgga gcccaaccac agcggcgcga cgcagttcga cctgatcctc
     1501 tcgatgtacg agctggacgg gagcctccac ttctcggccg actacagcag cgacctctac
     1561 gacgcgccga ccatggcccg gatgctcggc cagtacgcca acgtcctggc ggacgccgcc
     1621 cgccgccccg acgtccggct gtcgaggatc gccctggtcg gtgagcccga gcgccatcag
     1681 ctcctgcggg agtgggacga cacggagcac cgcttctcct cccagggcct ctgcattcat
     1741 catctcttcg ccgcgcaggc cgcggcgacg ccggaggctc ccgccgtctc tgcgggcggc
     1801 accccctgga cctacgccga gctcgaccgc cgctccagtg ccctggccgc ctctctacgc
     1861 cgtctgggcg tcggccccga gacccgggtc ggcctctacc tggagcgttc gccggagatc
     1921 gtggccgcgc tcctcgccgt cctcaaggcg ggcggcgcct atgtgcctct ggacccttcc
     1981 tatcccgagg accgcctgcg cttcatgctg gagcattcgg gggcccgggt ggtggtcacc
     2041 cagccctccc tctccggccg cctgggcggc gccgtccagg agctgaccct cggccccggc
     2101 acctgggacg cctctcctat cgaaggggag gagccgtacc cggacccgga gcatctggcg
     2161 tacctgatct atacctccgg ctccaccggc cggccgaagg gggtgatggt gaaccatcgc
     2221 accctcgtgg cctcgaccct ggcccggatg gcctactaca aagaggtgcc gcggagcttc
     2281 ctgctcctct cctcgttcgc cttcgacagc tccgtggcgg gcatcttcgg caccctgtgc
     2341 accggcggca agctggtggt ctctcctccc ggcctggccg agaacccgga acggctgacc
     2401 gagctgatcc ggcacgaaga ggtctccgac ttcctctgcg tcccctcgct ctactcccat
     2461 ctggtcgaga ggccggagct cgcggacatc cgcggcgtcg tcgtggccgg ggaagcctgc
     2521 ccggcggatc tggtggatct gcacttcagg ctgcacccgc gggccggcct gtggaacgag
     2581 tacggcccca ccgagggcac ggtgtggagc agcgtctacg cgttccgtcc ctgggaccgc
     2641 ctggaacggg tgcccatcgg ccgccccatc gccggcgtcc tcctctatgt gctggacgag
     2701 gcgggagagg tcgcgcccct gggagtgccc ggcgagctgt tcgtcggcgg cgcctgcgtg
     2761 gcccgcggtt atgaggggcg gccggagctc accgcggaga agttcgtgcc ggatccgttc
     2821 ggccctgagc ccggcggccg tctctaccgt accggtgacc tggtcaggta ccttcccggc
     2881 ggtgacctgc tgttcctggg gcgcatcgac cagcaagtga aggtccgcgg ctaccgcatc
     2941 gagctggagg agatcgaggc cgtgctgcgc cagcaccagg cgctgcgcga ggccgtggtg
     3001 gtgcctctgg aggccaggaa gaagggcgcc gaggcggcgg ataccggcgc gccccagtac
     3061 cagcgcctgg ccgcctacgt cgtcgcgcgc cagggcgagc ccgcgcccca ggccgcggcg
     3121 ctccgcgcct tcctcaagga gcggctgccc gagtacatga tcccgcacgg catcgtcgtc
     3181 ctgccggagc tgccgctcag cccgaacggc aaggtggacc gccgcgccct ggccgccgcc
     3241 ggcggcgagg cgttcgcccc cacggtcccc tacgtggcgc cgcgcaacgc catggagggg
     3301 cggctggtgg agatctggaa ggagctgctg gaggtggacc gggtcggcgt ccaggacagc
     3361 ttcttcgacc tgggggggca ctcgctgctc accacccggc tcatctcgcg ggtgcgtgac
     3421 gccttccagg tggaggtgcc gctccagacc tttttcgagg agccgaccgt cgccggcctc
     3481 gggcagagca tcgagctggc gcgctgggcc gaggaggtgg cccgggaggc gcccgccacc
     3541 gtgggcgccg aggggtacga ggagggcgag atctgagcgc cgccggcgga ccgcgcctca
     3601 gccgggacgt ccagcacgcc ctctcgcccc ggatcgccga ggcggcgctc gccgcctacg
     3661 atctgggaga ggtccagatc acccccctgg accgcaagaa gaacgtcacc ttcaggatcg
     3721 accggcccgc cggcggtcac gacggcgagc gcttcctcct gcggctctgc aaccccgggg
     3781 agtacgcccc cgaggaggtg ggctccgagc tggcctggct gctgcggctg cgccatgacg
     3841 aggggctcct cgtccccgag ccggtccgca cccgcgacgg cggcctgctc acccgcgccg
     3901 ccgtcgacga agccgccgag gccgccgctc cctggtgctg cgccctgttc cgctgggtcc
     3961 cgggccaggt ccttcagggg aacctcaccc ccgaggccgt gggcggggtg gggactctcc
     4021 tcgcccgtct ccaccgcgcc gcggagcgct tcgagccgcc gcccggcttc gcccgcccgc
     4081 gctgggactg cgaccgcctg ttcggcaccg ccgccgaagg ggaggtcgtg ccgcgcgata
     4141 tggatgaccc gctcgtctcg cggcgcgccc gggagatcct ggccgaggcc gcggccgtgg
     4201 tgcggcagac ggcggagggg ctggggcagg ggcccggcgt cttcggcatg atccacaagg
     4261 acctggagcc ggacaacatc gtgatctcgg acggcgcccc gtgcgccatc gatttcggcg
     4321 actgcggctg ggggtggttc ctctacgatg tggcggcctc gctcctcccg ctgcgggaga
     4381 agaaggggtt cgccgagatg cgggacgcct tcctcgccgg ctaccggagc gtccggccgc
     4441 tgacctccga agaggaggag ctggtcgaga ccttcactat cgcccgcagc ctgttcgcca
     4501 tccgcctcat gttgcgggag acgatgagca tccccaaggt acgggagtac gcccaggtcg
     4561 ccatccccca catgctgggg gagatccgcc ggttcctgga gcgccgggga gggttcgctg
     4621 gcgggggcga gtccgtggac gaggcggggc gcatgaccac gctccagttc ctgggccggc
     4681 tgcgggagct ggggatcaag gtctgggccg aaggggagag gctccgcttc tcggcgccgc
     4741 agggagccct cactcccgag ctcaaggccg agctggcgga gcgcaagcag gaggtgctgg
     4801 cgttcctgga tcagggctcc cgcgccgccg ggccggggcc catgcctccc gaggtggcgg
     4861 cgtccggtcg cctccccctg tccttcgccc aggagcgcct ctgggaccgg caccggctgg
     4921 agccgcggag cgccgccggc catgtggccc gggccgtgcg ccttgccggg ccggtgcggc
     4981 cggacctcct ggagcgcggc ctgcgccagg ccgtgcggcg gcactccatc ctgcgcacca
     5041 ccttcggcga ggcggagggc aggccctatc agaccgtggc ggcggtgacg gcgatcctgc
     5101 ccgtcgtgga cctggggagc ctctccgcgg aggcgcggca gccggaggcg gagcgcctcg
     5161 ccgcggagct ggcgcaccgg ccgttcgatc tcgagcgggg gccgctcctg cggctggtcc
     5221 tgctgcggct cgagccgcgg gagcacgtcg ccgtggccgt gctccacggc ctggtgggcg
     5281 acgactggtc ggccggcctc ctgtttcgcg agatcggggc cctctacgaa gcgtacgcca
     5341 cggagcgcgc cccccttctc cccgagccgc cgctccagta cgcggacttc gcgtggtggc
     5401 agcgcgagca cctccagggg gagacgctgg agaagccgct ggccttctgg cgacaggagc
     5461 tggccggcgc gccggcacgg gcggccctgc cgttcgaccg gccgtccgcc aggatagagg
     5521 gtagggggga gcgtcacgca tcgctgctcc cgcccgaggt catcgagccg ctccgcgagc
     5581 tgggcgagag ccaggggggc gcgaccctgt ccatggtgct cctggcggcc ttcgccgccc
     5641 tgctggcccg ggtctccggc caggacgacc tggtcgtggg atcccccttc gcccaccgcg
     5701 gccggccgga gctggacggg ctcatcgggc cgttcgtcaa cgctctggcg ctacgggtgc
     5761 gcttggcggg ggaccccacc ttccgcgagc tggtggggcg ggtgcggcag acggtgctcg
     5821 ccgcccaggc gcaccaggag ctgcccattg aggcgctgag ggacggcagt gttcatcatc
     5881 cgccgttctc ggtgacgttc agcctgcaga acgtgcccgc cccggacctc caggtggcga
     5941 ccttccgcat gaccctgctg ccgcccatcg cccgcgtggc gcaggtggac ctggcggtgg
     6001 acatgaagga catgccggag ggggtgcggg tgtcctggcg ctaccggccg gagctcttcg
     6061 aggccgccac cgtggcccgg ctggccgagg cttacctggc cttgctgaag gacgtggggc
     6121 ggtgtccgga tcggctatca tcagtcctcc tccccgcaga ggtgaccgcg gcctcatgag
     6181 ctccactccc ctcgcccagc ctctcgccca tcacgacgag gtcgaccgcc ggctcctggc
     6241 ggacttccag gagttccacg cgcgcctgcc cagccgcgac aaggtcatct acatgttctt
     6301 caccaccgac cttctccact ggctgagccg ggcgctgaag ttcgttcccg aggacgtgaa
     6361 cctggtcctg atcggctccg gcctgctgcc cgaggagatc gcctggatcg agcgccatgc
     6421 ccgccggccg ttccaccaca tcgcctcgcg ggtggacgac g
//
LOCUS       DASPJI010000247         8390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_701080, whole genome shotgun sequence.
ACCESSION   DASPJI010000247 DASPJI010000000
VERSION     DASPJI010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8390)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701080"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3098)
                     /locus_tag="VEL74_06505"
     CDS             complement(<1..3098)
                     /locus_tag="VEL74_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62213.1"
                     /translation="MSQDSFVSLGQRVSARREGLGSGFAGIHQRIALRAERAPEAPAL
                     VCGDRCLTFEDLEVRTAALARRLRALGVGPESIVGVHLERSPDAVVALLAVLRAGGAY
                     LPLDPSYPRERLSYMLADSGAAALVTREGLRGSLPQEVPLVLCVDGVGEGSGALLPEL
                     EEAGPESLAYVLYTSGTSGRPKGVGISHRSLAHHAEAVARAFGLGPRDRVLQFSSLSF
                     DVAAEEIYPSLLMGAAVVFVPESRGARGALGPQGIVPEDLGAFAERERLTVLNLPSPY
                     WHEWVDALERTGERLPSLLRLVVTGSEAVAPDKLAAWRRIVAAENERVVWMNAYGLTE
                     STVTSTAFRLPLRRPDLELSPVPVGRPLGEVRAHVLDAGGQPAAPDTVGELWLGGPGI
                     ARGYLGRPDLTAERFIPDPFSSLPGARLLRTGDLARLGPDGNLRLLGRADAQLKVRGF
                     RLEPEEIEAALRVHPAVRQAVVVARDERLTACLVMEGDAALTSSTLFELRDLLRQTLP
                     EHMVPGAFLSLERLPVTANGKVDRRALTALAAGAAPAAASAEDFHYEAPRTPVEEALC
                     GLWAALLGLKRVGVHDDFFHLGGHSLLATRLVSRVRSLFAVEIDLCDVFEAPAPAGLA
                     ARVEGAWRGVESPPLVPAPPAPYRPLSHAQRRLWFLYRFQGGSGAYNMPTLFRIRGGL
                     RRDALAAALDEVVRRHEALRTVFAMVDGVPVQMVMPFAPAPLPAVDLSALPVAEGRAE
                     AARLAAEDAQAPFDLGRGPLVRCTVLSLGADEHALLVNSHHIVSDGWSVGVLVRELIA
                     LYQAFAEGRPSPLPELAIQYGDFAVWQHEALDGPVMESQLGYWRQRLAGLPAALELPT
                     DRQRPPVQTFRGAVEHVALPAALARDLERVGRDIGRGQGATLFMTLLAGFQSLLSRVT
                     GQDDLLVGSPIANRTRAEVEDLIGFFVNTLVLRTDLSGGPTFSELLGRVRANALGAYA
                     HQDVPFERLVEEISPERDLSRTPLFQVVFGLHNSPMPRMELAPGLTLSLEEPHTETSK
                     FDL"
     gene            complement(3444..5486)
                     /locus_tag="VEL74_06510"
     CDS             complement(3444..5486)
                     /locus_tag="VEL74_06510"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HYG62214.1"
                     /translation="MSRGRDAAKRVPQNESELPDGWMDTVLEDLIVHALGGEWGEDPK
                     AELPEGAELVRVSVLRGTEFRQWDREKGATAAERLIKPSSLARRRLAPGDLVVEISGG
                     GPDQPVGRTLLVDQEAVEQAKNPLICSNFCRQIRLHPDIDPLYVQIVLRHRYLRGDFN
                     RYQAQTTNIRNLNFNEFLAGVSVPVPPLAEQRRIAGMAGDLLGRVDNARRRLERMPGI
                     LKRFRQAVLAAAYAGKLTEEWRRKGNGSLRLLRADLPAWELPSPLEMPEVPSAWEILP
                     LREVTSRVQYGLSLKADGEARGGIPMLRMGNIQDGRIDDSDLKYLDPEIRDLSSFLLA
                     RGDILFNRTNSPELVGKAAVYDLEREAVFASYLVRISCDERRVASRFLCGWINSPWGR
                     WWARTVRTDGVSQSNISTSKLQVMPVPVPPLEEQREIVRRVEALFELAESIERRVAVA
                     VASADRLTQAVLGAALRGVLVPAEATLATREGRDFEAAWTLLERVRAERENAGGATGG
                     QRGRRGRSRPEPGEPAAPPGPPRLEDLAAEQVLALFRQACWGAGEMSEEELFRRVARR
                     HGCKRLTKGLQARLREHLALAITRRIVGRAGDQLLVGATPNFARYEQEFLLQALLAVV
                     RKGAEYERTAVCRAIAARLGYSQVTAAMRERMEEIFGTAIRMGLLEARGGRLARRG"
     gene            complement(5483..6991)
                     /locus_tag="VEL74_06515"
     CDS             complement(5483..6991)
                     /locus_tag="VEL74_06515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HYG62215.1"
                     /translation="MSDVVQKLWGFCHTLRHDGIDYGDYIEQITYLLFLKMADERGID
                     LSRTRVTAQPGEDYVINCSWPCLALRSGPGLTEHYAGVLRSLGEQPGLLGDIFSGAAS
                     RFNNPQNLRKLIDLIDETEWIKLDVDVKAAAFEGLLEKAASEGKKGAGQYFTPRLLIQ
                     SIVRCVRPDPRAGAEFTVCDPACGTGGFLVAAYEWFMAEAGSAASGASPELIRRVRRG
                     TYFGSELVPRPRRLALMNLCLHGLEPEIRLGDSIYEPPGDRTFDVIFTNPPFGTRGAN
                     QIPGREDLAITTSNKQLNFLQHVLTTLKPGGRAAVVVPDNCLFADQAGEVFRILTRDC
                     DLHTVLRLPNGTFTPYSPGTKTNVVFFTKGSPTETVWVYDARTNVQRVTKKDRPLSAT
                     HLVAFEACFGTDPFGRSRRDPRDSWEGRWRPFSIAEVTARQFKIDSLKWLRDESLDDD
                     ELPEPLELADEALSELRDAILELNDVRKLLEPEKDESESESQKEATVLRGVR"
     gene            complement(7189..7857)
                     /locus_tag="VEL74_06520"
     CDS             complement(7189..7857)
                     /locus_tag="VEL74_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62216.1"
                     /translation="MRARDNPFAVHRVLAIRYRLAGDGCDWRGLERRLALLDFRAALV
                     GPHGSGKTTLLEDLGARLEARGARLRRLTLRRGERRPPPADERRLLDGLAPHDLLLVD
                     GADELGCAAWWRLRRASRGALGLLVTSHRAGLLPTLHEHVTTPGLLAAIVADLLGPAA
                     PPPPTPPEELFQRHGGDLRAALRELYDLYADHPSCPEGARPDMPGGSWTRRPPSPSRL
                     VQIE"
     gene            complement(7841..8230)
                     /locus_tag="VEL74_06525"
     CDS             complement(7841..8230)
                     /locus_tag="VEL74_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62217.1"
                     /translation="MSITIETTDGRRAFRPGDRIEGTASWYLEKEPRSVELRLFWYTQ
                     GKGTQDVEIVQGVPFDNPGAVDSRAFSVELPLAPYSFSGKLISLLWGLELVAEPGGET
                     GRLEITVSPTGVEILLRPDLAGDARPG"
BASE COUNT         1244 a         2971 c         3070 g         1105 t
ORIGIN      
        1 aggtcgaact tggaggtctc ggtgtgcggc tcctcgaggg acagggtgag gcccggcgcc
       61 agctccatgc gcggcatcgg cgagttgtgg aggccgaaga ccacctggaa gagcggcgtc
      121 cgcgacaggt cccgctccgg ggagatctcc tccaccagac gctcgaacgg cacgtcctgg
      181 tgggcgtagg cgcccagggc gttggcgcgc acccggccga gcagctcgga gaaggtggga
      241 ccgcccgaca ggtcggtgcg cagcaccagg gtgttgacga agaagccgat caggtcctcg
      301 acctcggccc gcgtgcggtt ggcgatgggc gagcccacca gcaggtcgtc ctggccggtg
      361 acgcgggaga ggaggctctg gaagccggcg aggagcgtca tgaagagggt ggcgccctgg
      421 ccgcggccga tgtcgcggcc cacccgctcc aggtcgcggg cgagcgccgc gggcagggcc
      481 acgtgctcga cggcgccgcg gaaggtctgg accggcgggc gctggcgatc ggtgggcagc
      541 tccagcgccg ccggcaggcc ggccaggcgc tggcgccaat agccgagctg cgactccatc
      601 accggcccgt cgagcgcctc gtgctgccag accgcgaagt cgccgtattg gatggccagc
      661 tcgggcagcg gcgacgggcg gccttcggcg aacgcctggt agagggcgat cagctcccgc
      721 accagcacgc ccaccgacca gccgtcggag acgatgtgat ggctgttgac cagcagcgcg
      781 tgctcgtccg ctcccaggga gagcaccgtg cagcgcacca gcggtccgcg gccgaggtcg
      841 aacggcgcct gggcgtcctc ggcggcgagg cgcgcggcct cggcgcggcc ttcggccacg
      901 ggcagggcgg agaggtcgac ggccggcagg ggcgcgggcg cgaatggcat caccatctgg
      961 accgggacgc cgtcgaccat cgcgaagacc gtgcgcaacg cctcgtgccg gcgcaccacc
     1021 tcatccagcg cggcggccag cgcgtcgcgc cgcaggccgc cgcggatgcg gaacagagtg
     1081 ggcatgttgt aggcgccgga gccgccctgg aagcgataga ggaaccagag gcgccgctgg
     1141 gcgtgggaga gcggccggta cggcgcgggc ggcgccggca ccagcggcgg cgactcgacg
     1201 ccgcgccagg ccccctcgac gcgggcggcg aggccggccg gggccggcgc ctcgaacacg
     1261 tcgcagaggt cgatctcgac ggcgaacagc gagcgcaccc gcgacaccag ccgggtggcg
     1321 agcagcgaat ggccgcccag atggaagaaa tcgtcgtgga cgcccacccg cttgaggccg
     1381 aggagcgccg cccagaggcc gcacagtgcc tcctccaccg gcgtgcgggg cgcctcgtag
     1441 tggaaatcct ccgcgctggc agccgccggg gccgcgccgg cggcgagggc ggtcagggcg
     1501 cggcggtcca ccttgccgtt ggcggtgacc gggagccgct ccagggagag gaaggcgccg
     1561 ggcaccatgt gctcggggag ggtctggcgc agcaggtcgc gcagctcgaa gagcgtcgac
     1621 gacgtcagcg cggcgtcccc ctccatcacc aggcaggccg tcagccgctc gtcgcgggcg
     1681 acgaccacgg cctggcgcac cgccgggtgg acgcgcaagg ccgcctcgat ctcctcgggc
     1741 tcgagcctga agccgcggac cttgagctgg gcgtcggcgc ggccgaggag acggaggttg
     1801 ccgtccggcc ccagacgcgc gaggtcgccg gtgcgcagca gccgggcgcc ggggagcgac
     1861 gagaacggat cggggatgaa gcgctcggcg gtgaggtcgg gccgccccag gtagccgcgg
     1921 gcgatgcccg ggccgcccag ccagagctcg ccgaccgtgt ccggggccgc cggctgcccg
     1981 ccggcatcca gcacgtgggc ccgcacctcg cccagcggcc ggcccacggg cacgggcgag
     2041 agctcgaggt cggggcggcg cagcggcagc cggaaggcgg tggaggtgac ggtggactcg
     2101 gtgaggccgt aggcgttcat ccagaccacg cgctcgttct cggcggcgac gatccgccgc
     2161 cacgcggcga gcttgtccgg cgccacggcc tcgctgccgg tcaccaccag gcggagcagc
     2221 gacggcagcc gctcgccggt gcgctcgagg gcgtccaccc actcgtgcca gtaggggctg
     2281 ggaaggttga gcaccgtcag gcgctcgcgc tcggcgaagg cgcccagatc ctcgggaact
     2341 atcccctgag gccccagggc gccgcgggcg ccgcggctct cgggcacgaa gaccacggcc
     2401 gctcccatca ggagagacgg gtagatctcc tcggcggcga cgtcgaagct cagcgaggag
     2461 aactggagga cccggtcgcg cggcccgagg ccgaaggcgc gggcgacggc ctccgcgtga
     2521 tgggcgaggg agcggtggga gatccccacc cccttggggc ggccgctggt gccggaggtg
     2581 tagaggacgt aggccaggct ctccggcccg gcctcctcca gctccggcag cagcgcgccg
     2641 cttccttcgc cgacgccgtc gacgcagagg accagcggca cctcctgcgg caggctgcca
     2701 cgcagccctt cgcgcgtcac cagcgcggcg gccccggagt cggccagcat gtacgacagc
     2761 cgctcccggg ggtacgaagg atcgaggggg agataagcgc cgccggcccg cagcaccgcg
     2821 aggagcgcca ccaccgcgtc gggcgagcgc tccaggtgta cgccgacgat cgactcgggg
     2881 cccacgccca gagcccgcag gcggcgggcc agggcggcgg tgcggacctc caggtcttcg
     2941 aaggtcaggc agcggtcgcc gcagaccagg gcaggcgctt caggcgcccg ctccgcccgc
     3001 agggcgatcc gctggtggat gccggcgaaa ccggagccca ggccttcgcg ccgagcggat
     3061 acacgttgtc ccagtgatac aaacgaatcc tgcgacattc ccctccctcc aaactacaag
     3121 agataactgc aaataaaatc cggaagcccc gaagccgcca cccgctggga gcgggcccga
     3181 tccttcaacg ctgccgggtt gttctagtag tgggctcact ccgccaaagc gggacaccac
     3241 acatgcggtg acgagccgcc cggtgaacgc ggtgacgtcc tccgtgcggt catcgcggcg
     3301 cagctacgaa gctactttcg agggggagct gaacggcgag cgctctgcga gggcgcgtat
     3361 gttgatccat cgcccatcgc ggcgcaacct tgtagaaact tgatcatttg atggtccgga
     3421 gccgtccagc aatagaagcg agctcagccc cgccgcgcca ggcggcctcc ccgcgcctcc
     3481 agcaggccca tgcggatcgc cgtgccgaag atctcctcca tgcgctcgcg catggcggcg
     3541 gtgacctggc tgtagccgag gcgcgcggcg atggcgcggc agaccgccgt gcgctcgtac
     3601 tcggccccct tgcgcacgac ggcgagcagg gcctggagca ggaactcctg ttcgtagcgc
     3661 gcgaagttcg gcgtcgcgcc gaccagcagc tgatcgcccg cccggccgac gatccgccgg
     3721 gtgatggcga gggcgaggtg ctcgcgcagc cgcgcctgga gccccttggt gaggcgcttg
     3781 cagccgtggc gccgggcgac gcgccggaac agctcctcct cggacatttc gccggcgccc
     3841 cagcaggcct gccggaagag cgccagcacc tgctcggcgg cgaggtcctc caggcgcggc
     3901 gggccgggag gcgcggcggg ctcgccgggc tcgggccggc tcctgccgcg gcggccgcgc
     3961 tggccgccgg tggcgccgcc ggcgttctcg cgctccgccc gcacgcgctc gagcagcgtc
     4021 catgcggcct cgaagtcgcg cccctcgcgg gtggccagcg tcgcctcggc cggcaccaac
     4081 acgccgcgca gggcggcgcc cagcaccgcc tgagtcagcc ggtcggcgct cgccaccgcc
     4141 accgcaaccc gccgctcgat gctctccgcc agctcgaaca gcgcctcgac gcggcggacg
     4201 atctcccgct gctcctcgag cggcggcacc ggcaccggca tcacctggag cttggaggtg
     4261 ctgatgttcg actggctgac gccgtcggtg cgcacggtcc gcgcccacca gcggccccag
     4321 ggggagttga tccagccgca gaggaaacgg ctggcgaccc gccgctcgtc gcaggagatg
     4381 cggacgagat aggaggcgaa gaccgcctcg cgctccaggt cgtacaccgc cgccttgccc
     4441 accagctcgg ggctgttggt gcggttgaac aggatgtcgc cgcgcgccag caggaagctc
     4501 gacaggtccc ggatctccgg gtcgaggtac ttgaggtcgg agtcgtcgat ccggccgtcc
     4561 tggatgttgc ccatgcgcag catcgggata ccgccgcggg cctcgccgtc cgccttgagc
     4621 gagagcccgt actgcacccg cgaggtcacc tcgcgcaggg ggaggatctc ccaggccgag
     4681 ggcacctccg gcatctccag cggggagggc agctcccatg ccgggaggtc cgctcgcagg
     4741 agccgcaggg agccgttccc cttgcggcgc cactcctcgg tcagcttgcc ggcgtacgcc
     4801 gcggcgagca ccgcctgccg gaaccgcttg aggatcccgg gcatccgctc gagccgccgt
     4861 cgcgcgttgt cgacgcggcc cagcaggtcc ccggccatgc cggcgatgcg ccgctgctcg
     4921 gcgagcggcg gcacgggcac ggagacaccg gccaggaact cgttgaagtt caggttgcgg
     4981 atgttggtgg tctgcgcctg gtagcggttg aagtcgcccc gcagataacg gtggcgcagc
     5041 acgatctgca cgtagagcgg gtcgatgtcc gggtgcaggc ggatctgacg gcagaaattg
     5101 gaacagatga gcgggttctt cgcctgctcg acggcctcct ggtcgaccag cagcgtcctg
     5161 cccaccggct ggtcggggcc gccgcccgag atctccacca ccaggtcgcc cggcgcgagc
     5221 cggcggcgcg cgaggctgga gggcttgatc agccgctcgg ccgccgtcgc ccccttctcg
     5281 cggtcccact gccggaactc ggtgccgcgc agcacgctca cccgcaccag ctccgcccct
     5341 tccggcagct ccgccttcgg gtcctcgccc cactcgccgc cgagggcgtg gacgatgagg
     5401 tcctccagaa ccgtgtccat ccacccgtcc ggcagctcgc tctcgttctg ggggacccgc
     5461 ttcgccgcgt cgcgcccgcg gctcatcgca caccccggag cacggtggct tccttctggc
     5521 tctcgctctc gctctcgtcc ttctccggct ccagcagctt gcggacatcg ttcagctcga
     5581 ggatggcgtc ccgcagctcc gacagggcct cgtccgccag ctccagcggc tcgggcagct
     5641 cgtcgtcgtc cagcgactcg tcgcgcagcc acttcaggct gtcgatcttg aactgccgcg
     5701 ccgtcacctc ggcgatggag aacgggcgcc agcgcccctc ccacgagtcg cgggggtcgc
     5761 gccgcgagcg gccgaacggg tcggtgccga agcaggcctc gaacgcgacc aggtgggtgg
     5821 cgctcagggg gcggtccttc ttggtgacgc gctgcacgtt ggtacgggcg tcgtagaccc
     5881 agaccgtctc ggtcgggctc cccttggtga agaagacgac attggtcttt gtgcccgggc
     5941 tgtatggcgt gaaggtaccg ttgggcaggc gcagcacggt gtggaggtcg cagtcgcggg
     6001 tgaggatgcg gaacacctcg cccgcctggt cggcgaacag gcagttgtcc ggcaccacga
     6061 ccgcggcccg cccgccgggc ttcaacgtcg tgagcacatg ctgcaggaag ttgagctgct
     6121 tgttggaggt ggtgatggcg agatcctccc gcccggggat ctggttggcg ccccgggtgc
     6181 cgaacggcgg gttggtgaag atcacatcga aggtacggtc gccggggggc tcgtagatgg
     6241 aatcgcccag tcggatctcg ggctcgagcc cgtgcaggca gaggttcatc agcgccaggc
     6301 gccgcggccg gggcaccagc tcggagccga agtaggtgcc gcgccgcacg cgccggatca
     6361 gctccggcga tgctcccgac gccgcgctgc cggcctccgc catgaaccac tcgtaggcgg
     6421 ccaccaggaa gccgccggtg ccgcaggccg gatcgcagac ggtgaactcg gcgcccgccc
     6481 gcgggtcggg gcgcacgcag cggacgatgg actggatgag caggcgcggc gtgaagtact
     6541 ggccggcccc cttcttgccc tcgctcgccg ccttctccag cagcccctcg aaggccgccg
     6601 ccttgacgtc cacgtcgagc ttgatccact cggtctcgtc gatcagatcg atcagcttgc
     6661 gcaggttctg cgggttgttg aaccgcgacg ccgcgccgct gaagatgtcc cccaggaggc
     6721 ccggctgctc gcccagggag cgcagcacgc cggcatagtg ctcggtcagc cccgggccgg
     6781 agcggagcgc gaggcacggc cacgagcaat tgatcacata atcttccccc ggctgcgccg
     6841 tcacccgcgt gcggctgagg tcgatccccc gctcgtccgc catcttgagg aagagcagat
     6901 aggtgatctg ttcgatgtag tcgccgtagt cgatcccatc gtggcgcagg gtgtggcaga
     6961 agccccacag cttctggacg acatcgctca tgaccgcgag aacccccttg aaagccggcg
     7021 cgtcggttcg cgtggagggc cggccggtcc ctgcccgtcc gcagaagtca ccgagcttgg
     7081 tgacaagacc cgtcctccgc tcacaaatcg gtgttgccga gacattacga cgaaagaatc
     7141 ggaaaactct ccgatcggcg aagtgtaacg cacaaacgtc ctggtgattc actcgatctg
     7201 cacgaggcgc gagggggacg gtggtcgccg agtccaggac ccgccgggca tgtccggacg
     7261 agccccttcc ggacaggatg gatggtccgc gtagaggtca taaagttcgc gcaaggcggc
     7321 tcggagatcc ccgccgtggc gctggaacag ctcctcgggc ggggtggggg gcggcggcgc
     7381 ggccggcccc aggagatcgg cgacgatcgc cgccagcagg ccgggggtgg tgacgtgctc
     7441 gtggagcgtg gggaggaggc ccgcgcgatg agaggtgaca agcaggccga gcgccccgcg
     7501 gctcgcccgg cgcagtcgcc accaagcggc gcagccgagc tcgtcggcgc cgtcgacgag
     7561 cagcaggtcg tgcggcgcga ggccgtccag gaggcgccgc tcgtccgccg gcggcggccg
     7621 gcgctcgccg cgccgcaggg tgagccggcg cagccgcgcc ccccgcgctt ccagccgcgc
     7681 ccccaggtct tcgaggagcg tcgtcttgcc actgccgtgg ggccccacca gggcggcgcg
     7741 gaagtccagg agggcgagcc gccgctccag accgcgccaa tcgcacccgt ctccggcgag
     7801 ccggtagcgg atggcgagga cccggtggac ggcgaaggga ttatcccggg cgcgcatcgc
     7861 ctgccaggtc cgggcggaga aggatctcca cccctgtagg agagacggtg atctccagcc
     7921 gtccggtctc gcccccgggc tcggccacca gctccaggcc ccagaggagc gagatcagct
     7981 tgccggagaa gctgtacggc gccagaggga gctccaccga gaaggcgcgg ctgtcgaccg
     8041 ctcccgggtt gtcgaacggc acgccctgga cgatctccac gtcctgcgtc cccttgccct
     8101 gggtatacca gaagagccgc agctccacgg accgcggctc cttctcgagg taccaggagg
     8161 cggtcccctc gatccggtcg cccggacgga aggcacgccg gccgtcggtg gtctcgatcg
     8221 tgatgctcac ggctcccacc cctccggcgc ccccttgggc cggatgatga agtcgtagtc
     8281 ctcgctgacg tcgggccagt tgtcctgctc ccccagcagc ttcagcttcc agacgatccg
     8341 gttgttcggc gccttgaagg agtgcatggt ggggaacggg acgacgaagg
//
LOCUS       DASPJI010000248         3823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_188835, whole genome shotgun sequence.
ACCESSION   DASPJI010000248 DASPJI010000000
VERSION     DASPJI010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188835"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VEL74_06530"
     CDS             complement(<1..808)
                     /locus_tag="VEL74_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HYG62218.1"
                     /translation="MDVIQHQDPTGETMVARWPSQGTAAIRLGSQLIVEESQQAVFFR
                     DGKALDTFGPGRHTLATQNIPILASVFGIPFGGKSPFQAAVVFVSTRTFLDLKWGTKE
                     PVAFRDQELAMVRLRAFGKFAVRVTNAQQFVNTVVGTRGLYSTEGVEDWFRDVIVSRL
                     TDVLGENLTSIFDLPRLYDELAMGLKSRVADDFTKYGIELVDLYVGAITAPEEVQKLI
                     DERSGMGAIGDMNTYLKFKTARAIGDAAQGGGGGGAGDTVGAGLGIGMGAG"
     gene            complement(850..1383)
                     /locus_tag="VEL74_06535"
     CDS             complement(850..1383)
                     /locus_tag="VEL74_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62219.1"
                     /translation="MTNGFEEAKAQRNALERLGAKIPGFRGFQDRELRRDVDKMQREH
                     LSAELGRIKSALREKARAYTDAAKLGALNGFDRLDRQLDGLSQAIRFSDYGATGFFDP
                     VKIGEAELQRLYDFDLSVLDDLALLEDAAGAVPAPGGEDPAPALDRMREQARALEDKW
                     KGRELVLGNVVKTSRGV"
     gene            complement(1435..2445)
                     /locus_tag="VEL74_06540"
     CDS             complement(1435..2445)
                     /locus_tag="VEL74_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015155092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HYG62220.1"
                     /translation="MNARVKTGFEMLKETFQEWKDDDALQLGAALAYYTIFSLAPMLL
                     VVIAVAGLVWQREAVQGEIVHQIQGLVGQQGAEAIQTMIANAGRQGEKSGILATIIAV
                     ATILFGATGVFTQLQSSLNHIWDVEPKPGAGVRGLLKTRLLSFGMILAIGFLLLVSLV
                     VSTALAAVGTYMTSLLPAGAEILMKALTFAVSFAVITVLFAMMYRFLPDVKIAWRDVW
                     VGATVTALLFTIGKFLIGLYLGNSSVASTFGAAGSVIVLLLWIYYSTQILFFGAEFTQ
                     VYATRYGSRIEPDKDAVRVQEVKVPVGEGEGGGREARRRDPSPRPAGGGGRTPAHGRG
                     RA"
     gene            2627..3070
                     /locus_tag="VEL74_06545"
     CDS             2627..3070
                     /locus_tag="VEL74_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62221.1"
                     /translation="MDLRTFLEVVGVVIVVALVAAAIVLAVAIRKLRRIRVPPDADFF
                     TTVRAVPLSLVVGLDLLDLGLDFLSTPIVWFILDRTNLKALRNTATVEALLPFTGPIP
                     TLTLAWIAARTLGLGRPYDPNVIETERVGDSYIPRTGRAGRAGRR"
     gene            3074..>3823
                     /locus_tag="VEL74_06550"
     CDS             3074..>3823
                     /locus_tag="VEL74_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HYG62222.1"
                     /translation="MRTCIIVNPNAGSVEDMAELQRRLLRLPETGVRFTDHEGHAEEI
                     ARAAVRDGVERVVAAGGDGTLNEVVNGLAEGFGRVRLGLLPLGTGNDFARSINVPADL
                     EGALAVLEEGRAKPLDVAVVTFAGRTRYFINMAAGGFGGEVSEKAAEAKERWGPLAYM
                     RAALGALGEIQGFETTLTLNNAETLDLDTYNVVLSNGRYVAAGIPAAPQAVLDDGLLE
                     VMIVPATTVPQIAMLVPTVLLGLHTGSDLLLF"
BASE COUNT          577 a         1316 c         1326 g          604 t
ORIGIN      
        1 ggcccgcacc catcccgata cccaggccgg cccccaccgt atcgccggcg ccgccgcctc
       61 ctccaccctg ggcggcatcg ccgatggcgc gggcggtctt gaacttgagg taggtgttca
      121 tgtcgccgat ggcgcccatg ccgctgcgct cgtcgatgag cttctgcacc tcttcgggcg
      181 cggtgatggc gccgacgtag aggtccacca gctcgatgcc gtacttggtg aagtcgtcgg
      241 cgacgcgcga cttcaaaccc atcgccagct cgtcatagag ccgcggcagg tcgaaaatcg
      301 aggtcaggtt ctcgcccagg acgtcggtga gccgggagac gatcacgtcg cggaaccagt
      361 cctccacccc ctcggtgctg tagaggccgc gggtgcccac cacggtgttg acgaactgct
      421 gggcgttggt gacgcgcacc gcgaacttgc cgaaagcgcg caggcgcacc atggcgagct
      481 cctggtcgcg gaaggccacc ggctccttgg tcccccactt gaggtcgagg aaggtgcggg
      541 tggagacgaa gacgaccgcc gcctggaagg gggacttgcc gccgaaggga atgccgaaga
      601 cgctggccag gatggggatg ttctgggtgg ccagggtgtg gcggcccggg ccgaaggtgt
      661 ccagggcctt gccgtcgcgg aagaagacgg cctgctggct ctcctcgacg atgagctggc
      721 tgccgagccg gatggccgcg gtcccctggc tcggccaccg cgccaccatc gtctcgcccg
      781 tggggtcctg atgctggatt acgtccatgg ctctcgatct cctctgccga tctctaccga
      841 aggtgaacgt cagacgccgc ggctggtctt gaccacgttg ccgaggacca gctcgcgccc
      901 cttccacttg tcttccagcg cccgggcctg ttcgcgcatc cggtcgaggg ccggcgccgg
      961 gtcctcgccg ccgggggcgg ggacggcccc cgcggcgtct tccagcagag ccagatcgtc
     1021 gaggacggag aggtcgaagt cgtacaggcg ctgcagctcg gcctcgccga tcttcaccgg
     1081 atcgaagaag ccggtggcgc cgtagtcact gaagcggatc gcctgcgaca ggccgtcgag
     1141 ctgccggtcg aggcggtcga agccgttgag cgccccgagc ttggcggcgt cggtgtaggc
     1201 gcgcgccttc tcccgcagcg ccgacttgat acggcccagc tccgccgaca ggtgctcgcg
     1261 ctgcatcttg tcgacgtcgc gccgcagctc gcggtcctgg aacccccgga agccagggat
     1321 cttcgccccc agcctctcca gggcgttgcg ttgcgccttg gcctcttcaa agccgttcgt
     1381 catcgatctc cctcctcgcg gctcgtggag ggagatcgta tcagacacgg gttcctaggc
     1441 ccggccccgc ccatgcgccg gggtccggcc gccgccgcca gcgggtcggg ggctggggtc
     1501 acgccgccgc gcctcccggc cgccgccctc gccttccccg accggcacct tcacctcctg
     1561 gacccgcacc gcgtccttgt ccggctcgat gcgcgagccg tagcgcgtgg cgtagacctg
     1621 ggtgaactcg gcgccgaaga agaggatctg ggtcgagtag tagatccaga gcagcagcac
     1681 gatcaccgaa ccggcggcgc cgaaggtgga ggcgacgctg ctgttgccga ggtacaggcc
     1741 gatgaggaac ttgccgatgg tgaacagcag ggcggtcacc gtggcgccga cccagacgtc
     1801 gcgccaggcg atcttcacgt ccggcaggaa ccggtacatc atggcgaaga gcacggtgat
     1861 caccgcgaag gagacggcga aggtgagagc cttcatcagg atctcggcgc cggccggcag
     1921 cagcgaggtc atgtaggtgc cgacggcggc aagggcggtc gagaccacca gcgagaccag
     1981 cagcaggaag ccgatggcca ggatcatgcc gaaggagagg aggcgggtct tgagcagccc
     2041 gcgcacgccg gcgccgggct tcggctccac gtcccagatg tggttgaggc tgctctggag
     2101 ctgggtgaag acgccggtgg cgccgaacag gatggtggcg acggcgatga tggtggccag
     2161 gatcccggat ttctccccct ggcggccggc gttggcgatc atggtctgga tcgcctccgc
     2221 gccctgctgg ccgaccagcc cctggatctg atggacgatc tcgccctgga ccgcctcgcg
     2281 ctgccagacc aggccggcga cggcgatgac caccagcagc atgggggcga gggagaagat
     2341 ggtgtagtag gccagcgccg ctccgagctg cagggcgtcg tcgtccttcc attcctggaa
     2401 ggtctccttc agcatctcga agccggtttt cacacgagcg ttcatgttgg gtcctccttg
     2461 caagctcgag ctcgtagtcc ggcgccgacc aggtagacgc gggaggcgtg ccaccttggc
     2521 gcagtgcctc ccctatgcag gatcggggcc cgcccgggcc ctcttcctgg gggtgatcac
     2581 acttcttgca tggagcaggg caggagagga ggcccgagga gactccatgg acctgcgaac
     2641 gtttctcgag gtggtgggag tggtgatcgt ggtggccctg gtcgcggcgg ccatcgtgct
     2701 ggcggtcgcg atccgcaagc tgcggcggat ccgcgtgcct cctgacgccg acttcttcac
     2761 caccgtgcgg gcggttcccc tgtcgctggt cgtcgggctc gacctcttgg acctcgggct
     2821 cgacttcctg tcgacgccca tcgtctggtt catcctggac cgcaccaacc tcaaggcgct
     2881 gcgcaacacc gccacggtcg aagcgctgct ccccttcacc gggccgatcc cgacgctgac
     2941 tctggcctgg attgccgccc gcaccttggg gctcggccgc ccgtacgacc cgaacgtcat
     3001 cgagaccgag cgcgtcgggg acagctacat tccccgcacc ggccgcgccg gccgcgccgg
     3061 ccgccgttga gccatgcgca cctgcatcat cgtcaacccg aatgccggca gcgtcgagga
     3121 catggccgag ctccagcggc ggctcctgcg cctgcccgaa accggcgtcc gctttaccga
     3181 ccacgagggg catgccgagg agatcgcccg cgcagcggtg cgggacggag tcgagagggt
     3241 ggtggcggca ggcggtgacg gcacgctgaa cgaggtggtc aacggcctgg ccgagggctt
     3301 cggccgcgtc cgcctcggcc tgctgccact ggggacgggc aacgacttcg cccgctccat
     3361 caacgttccg gcggacctgg aaggggccct cgcggtgctg gaggaggggc gcgccaagcc
     3421 gctcgacgta gccgtcgtga ccttcgccgg ccgcacccgc tacttcatca atatggcggc
     3481 cgggggcttc ggcggcgagg tgagcgagaa ggccgccgag gccaaggagc gctgggggcc
     3541 gctggcctac atgcgggcgg ccctcggggc gctgggagag atccagggct tcgagacgac
     3601 cctcaccctg aacaacgccg agaccctgga cctcgacacc tacaacgtcg tcctgtcgaa
     3661 cggccgctac gtggccgccg gcatcccggc cgcgccgcag gcggtgctcg acgacggcct
     3721 gctcgaagtg atgatcgtgc ccgccaccac agtcccgcag atcgcgatgc tggtccccac
     3781 agtccttctg ggtctccaca ccggcagcga cctcctgctc ttc
//
LOCUS       DASPJI010000249         2401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_587595, whole genome shotgun sequence.
ACCESSION   DASPJI010000249 DASPJI010000000
VERSION     DASPJI010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587595"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2401)
                     /locus_tag="VEL74_06555"
     CDS             complement(<1..>2401)
                     /locus_tag="VEL74_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYG62223.1"
                     /translation="AVRHRPVANLVEWALRAFAFGPGDRVLFVTALSFDLSVFDVFGL
                     LAAGGSIHVASRRDLEEPERLVHLLLEEPITFWDSAPAALLQLAPLFPEPAAESRLRL
                     VFFSGDWIPLALPGRVRAAFPGARVVSLGGATEATVWSNVFPVEEIQPHWVSIPYGRP
                     IQNARYHILDGSLLPCPVGVPGDLYIGGGCLSSGYAAAPDLTADRYLPDPFGREAGGR
                     LYRTGDRARFFADGNIEFLGRLDHQVKIRGFRIELGEIEAALCQHEAVREAVVLVRED
                     VPGQRRLVAYVTPRRETAPGDEELRRFCGSRLPDYMVPAAFVALGLFPVTPNGKLDRA
                     ALPAPEDDGAGAGRALAAPRTSMEQAVAEVWAAVLGREAVGIHDGFFALGGDSLLAIQ
                     LIMRLRERLGVELPLRALFEHPTVADLALAVARARGEGGGGSLRVVTPAPAALHEPFP
                     LTDLQQAYWLGQRADFDFGGMSAHVYQERDFTDLDPARLETAWRRLVERHPMLRAVVL
                     PDGRQRVLPEVPPYAVEVLDLRALGSEAAAAEAATVGRRMADQGPATDAWPLFEVRLS
                     RLDERRWRLHVSLSLLISDALGLNVLQGELRRLYGDPGADLPPLDLTFRDYVLARAGD
                     EGSAAFARDRGYWLERLPSLPPAPELPLRSTARAEEARFIHHGWHLEPEGWRRFRERA
                     ARAGVTPTAAVLTAYAQTLAAWSKDPRFTLNVLYFNRLPVHPQVDRVVGNFSSTLPVA
                     VDAGGSASFEERAQRLQERLWQDLEHGRFSGVRVVRELARLHGVSSRPVLPVVFTSDI
                     NL"
BASE COUNT          363 a          864 c          920 g          254 t
ORIGIN      
        1 aggttgatgt cgctggtgaa caccaccggc agcaccgggc gcgacgagac gccgtgcagg
       61 cgggccagct cgcgcaccac ccgcacgccc gagaagcggc cgtgctcgag atcctgccag
      121 aggcgctcct ggaggcgctg cgcccgctcc tcgaagctgg cgctcccccc ggcgtcgacc
      181 gccaccggca gcgtggagct gaagttgccc accacccggt ccacctgcgg gtggacgggc
      241 aggcggttga agtagaggac gttcaaggtg aagcggggat ccttgctcca cgccgccagg
      301 gtctgcgcgt aggcggtcag caccgccgcg gtgggggtga cgccggcacg ggcggcgcgc
      361 tcgcggaagc gccgccagcc ctccggctcg aggtgccagc cgtggtggat gaagcgcgcc
      421 tcctccgccc gggccgtgct ccgcaacggc agctcgggag ccggcggcag cgacggcagg
      481 cgctccagcc agtagccgcg gtcgcgggcg aacgccgccg agccctcgtc gccggccctg
      541 gccaggacgt agtcccggaa ggtgagatcg agggggggca ggtccgcgcc cggatctccg
      601 tagagacggc gtagctcccc ctggaggacg ttcaggccca aggcgtccga gatcagcagg
      661 ctgaggctga cgtgcaggcg ccagcggcgc tcgtcgagac gcgacaggcg gacctcgaac
      721 agcggccagg catccgtggc cggcccctga tcggccatgc ggcggccgac ggtggcggct
      781 tcggccgcgg ccgcttcgga gcccagggca cgcaggtcca ggacctcgac ggcgtacggc
      841 ggcacctcgg gcagcacccg ctggcggccg tcgggcagga ccacggcgcg cagcatgggg
      901 tggcgctcca ccaggcggcg ccaggccgtt tccagccgcg ccgggtcgag gtccgtgaag
      961 tcgcgctcct ggtagacgtg ggccgacatg ccaccgaagt cgaagtcggc gcgctggccc
     1021 agccagtagg cctgctgcag gtcagtcagg gggaagggct cgtggagcgc cgccggggcc
     1081 ggcgtcacca cgcggagcga gccgccgccc ccctcgccgc gcgcccgcgc cacggccagc
     1141 gccaggtccg ccaccgtcgg gtgctcgaac agggcgcgca gcggcagctc cactcccaat
     1201 cgctcgcgca gccgcatgat gagctggatg gcgagcagcg agtcgccgcc caaagcgaag
     1261 aagccgtcgt ggattcccac cgcctcgcgg ccgaggacgg cggcccagac ctcggccacc
     1321 gcctgctcca tggaggtgcg gggcgccgcc agggcgcggc cggcgccggc gccgtcgtcc
     1381 tcgggggcgg gcagggcggc gcggtccagc ttgccgttgg gggtcaccgg gaagaggccg
     1441 agggcgacga aggcggcggg caccatgtag tccgggagcc gcgacccgca gaagcggcgc
     1501 agctcctcgt ctcccggcgc cgtctcccgg cgcggcgtca cgtaggcgac caggcgccgc
     1561 tgccccggca cgtcctcccg caccagcacc acggcctcgc gcaccgcctc gtgctggcac
     1621 agcgccgcct cgatctcccc cagctcgatg cggaagccgc gaatcttcac ctggtggtcg
     1681 aggcggccga ggaactcgat gttgccatcg gcgaagaagc gggcgcggtc gccggtacga
     1741 tagaggcgcc ccccggcctc ccgtccgaac ggatcgggca gatagcgatc ggcggtcagg
     1801 tccggggccg cggcatagcc ggaggacagg cagccgccgc cgatgtagag gtcgccgggc
     1861 acccccaccg ggcaggggag gagcgagccg tcgaggatgt ggtagcgggc gttctggatc
     1921 ggccggccgt aggggatgct cacccagtgg ggctggatct cctccaccgg gaagacgttg
     1981 gaccagaccg tggcctcggt cgcgcccccc aggctcacca cccgggcgcc ggggaaggcg
     2041 gcgcggacgc ggccgggcag ggccaggggg atccagtcac ccgagaagaa gaccagccgc
     2101 agccgactct ccgcggccgg ctcgggaaag agcggcgcga gctgcagcag cgccgccggc
     2161 gccgagtccc agaaggtgat cggctcctcc aagagcaggt ggaccaggcg ctccggctcc
     2221 tccaggtcgc gccgggaggc gacatggatc gacccgccgg cggccagcag tccgaagacg
     2281 tcgaagaccg acaggtcgaa ggagagggcg gtgacgaaca gcacccggtc cccgggcccg
     2341 aaggcgaagg cccggagggc ccactccacc aggttggcca cggggcggtg gcgcacggcg
     2401 a
//
LOCUS       DASPJI010000250         3630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_248889, whole genome shotgun sequence.
ACCESSION   DASPJI010000250 DASPJI010000000
VERSION     DASPJI010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248889"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VEL74_06560"
     CDS             complement(<1..757)
                     /locus_tag="VEL74_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYG62224.1"
                     /translation="MPPHQDETWREHLLADTQTPCAVEAVVPSRIPGLTILAHPQAGR
                     IGERVSLPDLWAGTSVSLSRLEPGFAHPGGTVRTALGTVHLSRSPILLHPAGDGGIRL
                     SCRETRTPVEADQEPVLNERTFTADDLARGVVLLLARSVALLLHSFCPVFQRIPDHGL
                     KGKSDAVQEIRSKIGTVAGHDVAVLLRGETGTGKELVARAIHQASPRRDRPFVAVNMG
                     AIPPSLAAAELFGSEKGSFTGADRRRQGLFSRAH"
     gene            988..2037
                     /locus_tag="VEL74_06565"
     CDS             988..2037
                     /locus_tag="VEL74_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62225.1"
                     /translation="MIFVYLLFSLLCSAIREGIAGLLKTRAVHLQRGIQRLLHDSAKN
                     GLADTLYKHPLISSLRRGDKDNRWNLPSYIPAKSFSLALMDLAVRGKDLANTASAGPA
                     APWISLDGLRASIQNLENVQVQRALLPLIDSAGGDLAQAQANIEAWYDSSMDRVAGWY
                     KRRTQAVLFAIGLGASALINVDTIKIATTLYYNDTLRDAVVARAADADQDALRQANAS
                     QLYAQLDQFDLPIGWDDDARPWSAPPAGRPESAWWRLAPILGWLITALAVTLGAPFWF
                     DLLNKIMVIRSTVKPHEKSPEESSEDRQKPSSSQGLSGGGPATAVSRSSTPAAGAPFF
                     QPHEWAGGHPQEGVL"
     gene            2034..2519
                     /locus_tag="VEL74_06570"
     CDS             2034..2519
                     /locus_tag="VEL74_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HYG62226.1"
                     /translation="MSAGAGNTVPSVNRDLDLLAPKFRDQVVAAIQACEDAGLDAYVY
                     EGYRSQELQALYYARGRTIIPPTKPVTNASSNLYSWHGYGLAVDVISRSKLWDRPESW
                     FAQVAAIFRQHDCRWGGEWKMRDLPHFQWGRCKPSPSDLARHLISTQGLEAVWRAVGA
                     V"
     gene            complement(2540..>3630)
                     /locus_tag="VEL74_06575"
     CDS             complement(2540..>3630)
                     /locus_tag="VEL74_06575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HYG62227.1"
                     /translation="ACVEVIVREIGRLGLETELLEVPHGLAGQGPVHTVRSFHGSGRR
                     TLYFHGHYDVVPAQSREQFEPREKGGKLFGRGSADMKSGLVAMIHAVKALKDCKVELD
                     GRIGLLMVPDEETGGARGSGPLTRAGILGEDGIGMLTPEPSSGVVWNANRGAITLRVT
                     VKGKPAHVGLHFKGKNAFEGMIAVAGRLLDLKREVETRATGFRIAPEAARRSILLLGG
                     ECAGGSNFNVVPERMSFTIDRRINPEEDLETEKGRLLEVLAGLTREGIDLEWEIFQEG
                     RPSGVPEDAPLSRALALSAEEVTGRRPEFEICPGLLENRFYAERGVPALAYGPGLLSV
                     SHGPNEFVVLEDVLDCATTYALTAARLL"
BASE COUNT          591 a         1268 c         1204 g          567 t
ORIGIN      
        1 cgtgggcacg gctgaacaat ccctgccgcc gccggtcggc cccggtgaag gagcccttct
       61 cggagccgaa gagctccgcc gccgccaggg agggggggat ggcccccatg ttgacggcaa
      121 cgaacggacg gtcgcggcgc ggactggcct gatggatggc ccgcgcgacc agctccttgc
      181 ccgtgccggt ctcgccgcgc aggagcaccg cgacatcgtg gccggccacg gtaccgatct
      241 tcgaacggat ctcctggacg gcgtcgctct ttcccttgag cccgtggtcg gggatccgct
      301 ggaacacggg gcagaacgaa tggaggagaa gggcgacgct ccgggcgagc aggaggacga
      361 cgccgcgcgc cagatcgtcg gccgtgaagg tcctctcgtt caggacgggc tcctgatccg
      421 cctccaccgg ggtgcgggtc tcccgacagc tcaggcggat gccgccgtcg ccggcggggt
      481 ggagcaggat cgggctgcgg ctcaggtgca cggtcccgag cgccgtgcga acggtcccgc
      541 caggatgggc gaagccgggc tcgagacggg agaggctgac gctcgtgccg gcccagagat
      601 ccggcaggct gacccgctca ccgatcctgc cggcctgcgg gtgcgcaagg atggtcaggc
      661 cggggatcct cgaggggacg acggcctcca ccgcgcaagg ggtctgcgta tcggcgagca
      721 gatgctcgcg ccaggtctca tcctgatgag ggggcacgac agcagtttag cgcaagtcag
      781 agctgaccgg cgcctcccca tcctttatac tgttgccaaa ccaattgaac cctccttcga
      841 gcggccggga agaagccgcc gcccgtccgt ctatcgagga gtggcccggg agctggtcca
      901 gccaccaacg cgagcctccg cctggcgggg gctcaaacca tgggagcgcc tatgctcggc
      961 tctacgattc tggatgtagc ggtcggattg atcttcgtgt atctgctttt cagcctgctc
     1021 tgctcggcga tccgggaagg catcgcgggg ctgctgaaga cccgggcggt ccatctgcaa
     1081 cggggcatcc agcggctcct ccacgactcc gccaagaacg gcctggcgga caccctgtac
     1141 aagcatcccc tcatcagcag cttgcgcagg ggggacaagg acaaccggtg gaaccttcct
     1201 tcctacattc cggcgaagag cttcagtctc gccctgatgg atctcgccgt ccgcggcaag
     1261 gacctcgcca acacggccag cgccggacct gccgcgccct ggatctccct ggacgggctg
     1321 cgggcctcga tccagaacct cgagaacgtt caggtgcagc gggcgctgct gccactgatc
     1381 gactccgccg gcggcgacct cgcccaggcg caggcgaaca tcgaggcgtg gtacgacagc
     1441 tccatggatc gggtcgccgg ctggtacaag cggcggacgc aggcggtcct gttcgccatc
     1501 ggcctggggg cgagcgccct catcaacgtc gatacgatca agatcgccac caccctgtac
     1561 tacaacgaca cgttgcgcga tgcggtcgtc gcgcgggcag cggacgccga ccaggacgcc
     1621 ctgcggcagg ccaatgcctc gcagctctat gcgcaactcg atcagttcga cctgccgatc
     1681 ggatgggacg acgacgcccg gccctggtcg gcgccgccag ccggccggcc ggagagcgcg
     1741 tggtggcgcc tcgctccgat tctgggctgg ttgatcaccg ccctggcggt caccctcggc
     1801 gcccccttct ggttcgacct gttgaacaag atcatggtca tccgctcgac cgtgaagccg
     1861 cacgagaaga gcccggagga gtcctccgaa gaccgtcaaa aaccttcctc atcccagggc
     1921 ttgtccggcg gcgggcctgc cacggccgtt tcgcggtcca gcacgccggc cgccggcgcg
     1981 ccgttcttcc aacctcatga atgggccggg ggccatcccc aggagggagt cctgtgagcg
     2041 ccggtgcagg gaataccgtg ccgtcggtga accgggacct ggatctgctg gcgccgaagt
     2101 tccgtgatca ggtagtggcc gcgatccaag cctgtgagga tgcggggctg gacgcctacg
     2161 tgtacgaagg ctaccgctca caagagctgc aggcgctcta ttacgcgcga gggcgcacca
     2221 tcatcccgcc caccaaaccg gtgacgaacg cctccagcaa cctgtacagc tggcacggct
     2281 acggactggc ggtggacgtc atctccagat ccaagctgtg ggaccgcccg gagagctggt
     2341 tcgcccaggt ggccgcgatt ttccggcagc acgactgccg gtggggcgga gagtggaaga
     2401 tgagggacct gccgcacttc cagtgggggc gctgtaagcc gagcccctcg gacctcgctc
     2461 gccatctgat cagcacccag ggcctcgagg cggtttggag ggccgtcgga gcggtttgac
     2521 caggccggcg cttgacactc taaagcaggc gcgccgccgt caacgcgtag gtggtggcgc
     2581 agtcgaggac atcctcgagg accacgaact cgttcggccc gtgggagacg gagaggagac
     2641 ccggcccata ggccagggcc ggaacccctc tctccgcgta gaagcggttc tcgagcaggc
     2701 ccgggcagat ctcgaactcc ggcctccggc cggtcacctc ctcggcgctc agggcgagcg
     2761 cccgggacaa aggggcatcc tccggaactc ccgaaggcct cccctcctgg aaaatctccc
     2821 attcgagatc gattccttcc cgggtgagcc ccgcgagcac ctccagaagc cggcccttct
     2881 ccgtctccag atcctcttcg ggattgatgc ggcgatcgat cgtgaaggac atccgctcgg
     2941 ggaccacgtt gaagttcgat cccccggcgc attcccctcc gagcagcagg atcgacctcc
     3001 gcgccgcctc cggggcgatg cggaagccgg tggcgcgcgt ctccacctcg cgcttgagat
     3061 cgaggagccg gccggccacg gcgatcattc cctcgaaggc gttcttcccc ttgaagtgca
     3121 gccccacgtg ggccggcttc cctttgaccg tcacccgcaa ggtgatggcg ccgcggttgg
     3181 cgttccagac gacgccgctc gacggctcgg gagtgagcat gccgatgccg tcctcgccca
     3241 ggatgcccgc ccgggtgaga ggacccgagc cccgcgctcc ccccgtctcc tcgtccggca
     3301 ccatcaggag gccgatccgt ccgtccagct ccaccttgca gtccttcagg gccttcacgg
     3361 catgaatcat cgccacgagg ccgctcttca tgtccgccga gccgcggccg aagagcttgc
     3421 cccccttctc ccgcggctca aactgttcgc ggctctgcgc cgggacgacg tcatagtggc
     3481 catggaagta gagggtccgc cgcccgctcc cgtgaaagct ccgcactgtg tggacggggc
     3541 cctggcccgc gagcccgtgc ggtacctcga gcagctcggt ctcgaggccg agcctcccga
     3601 tctcgcggac gatcacctcc acgcaggccg
//
LOCUS       DASPJI010000251         3878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_820411, whole genome shotgun sequence.
ACCESSION   DASPJI010000251 DASPJI010000000
VERSION     DASPJI010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820411"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VEL74_06580"
     CDS             <1..989
                     /locus_tag="VEL74_06580"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HYG62228.1"
                     /translation="GVQDGGEAGLDGVSVELLDGAGNIVATTTTSGDGGYSFQYLRPG
                     TYTVRVVSATLPTDYVQTYDLDGTSSPHQATVSLGAGEARVDLDFGYRYPPTGSIGDR
                     VWNDLDGNGVQEAGEDGVNGVTVQLLNAGGTVIATQVTSGNGNYSFTNLPAGTYSVRV
                     VSSTLPANFIQTYDLDGTGTAHTAAVALAAGEARTDVDFGYRANPAFQPGTGTIGYWK
                     THPEAWPVQQITIGGVTYTKAQAIYWMDMASRGDKTIDLFKQLVATRLNVILGNDASC
                     VSSSMYNAGSWLATNPLGSNVRSNSAAWLTGGPLHQRLDDYNNGRLCAPHRG"
     gene            1121..1330
                     /locus_tag="VEL74_06585"
     CDS             1121..1330
                     /locus_tag="VEL74_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62229.1"
                     /translation="MRKMGEEKQLGRECASGGQELLVSREGNRLEGPRPYRRPDLVVY
                     GRLSELTRLDGNVEFDGIAGSQTVT"
     gene            1379..3055
                     /locus_tag="VEL74_06590"
     CDS             1379..3055
                     /locus_tag="VEL74_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HYG62230.1"
                     /translation="MITWERIPGTAPRPIASCPSPTSGGLRCLWHGGFIANRRDLELE
                     TGFAGASDADLLLEIYRREGLQTPRRIAGTFAWILWDGDAQRLLAARDRLGTRGLFFR
                     LFSRSICLAERVGALRREPPSPDLNPVAVVCHIQGETPPPGETFLTGIHAVEPGTVLV
                     VEPERLSSQFYWRPAPHPSLGFPSDEAYADACREILFRVTAEYAGPTAGITLSGGLDS
                     TAVAAALRAARPEDELRAVSWISPELPEADESREIEAVVARLRLTLIPVAADQHWPLS
                     EGIDAIPDTPHYNFYTTLWDATFRTAREHGVTTLFSGMSGDHLFGGGLPPYSDLLLRG
                     RVGRMAAEIRAGLRQAPGLPRLLRSRVLGPIARSLTPWRDEYRPPPVSWLGEASQDLV
                     RQTRPELPHLLMPGRRERLRILRDPLLPAVALLIEHQASRHGIELRHPLLDHRLFDFA
                     AALPTEQTWSGGLKKAILRRALGDSLPREVTERPRKVYPVAIFHRGLREREQSKAWAL
                     MTDMRAAEMGFVDEPKLRDEYRRYLSGETQRALFWHTLTLEAWLRRYFPR"
     gene            3052..>3878
                     /locus_tag="VEL74_06595"
     CDS             3052..>3878
                     /locus_tag="VEL74_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62231.1"
                     /translation="MIAPEEHPDTSQERLRQLAASLRELRGTLRRVDYRQAEADCELL
                     ATALLQEIPRAELDRFRFAAIPRGGLIVLGMLAYALDLNPEHLQPHAGDGALVLVDDC
                     ALTGARFASHLAKTQAREVVFVHLYSHPALRQAIREQEERVRACLAAHDLRDTRGVAD
                     PEWQERWHRRLGGQRYWYGLPELVCFAWNEPDRPFWNAAAERVEDGWKVIPPHLCLKT
                     RIALAGPCPVPARRTWQSPADVVSGEFDGTLWLCQTTTDRVYSLDGVGAEIWRTLAS"
BASE COUNT          626 a         1392 c         1249 g          611 t
ORIGIN      
        1 gtggcgtcca ggacggcggc gaagcgggcc tcgatggtgt ctccgtcgag ctgctcgacg
       61 gcgccggcaa catcgtggcg actacgacga cgagcggtga cggcggctac tcgttccagt
      121 acctgcggcc cggcacctac accgtccgtg tcgtcagcgc caccctgccc accgactacg
      181 tccagaccta cgacctcgac ggcaccagct ccccgcacca ggcgacggtc tcgctgggcg
      241 ccggcgaagc ccgcgtcgac ctcgacttcg gctaccgcta cccgcccacc ggctcgatcg
      301 gtgaccgggt atggaacgac ctggatggca acggcgtcca ggaggccggg gaggacggcg
      361 tcaacggcgt caccgtgcaa ctgctcaacg ccggtggcac cgtcatcgcc acacaggtga
      421 ccagcggtaa cggcaactac tcgttcacca acctgccggc cggcacttac agtgtccgcg
      481 tcgtcagcag caccctgcct gccaacttca tccagaccta cgacctggac ggcaccggca
      541 cggcccacac cgcggcggtg gcgctggcgg caggggaggc ccgcaccgac gtcgacttcg
      601 gctaccgcgc caatccggcc ttccagccgg gcaccggcac catcggctac tggaagaccc
      661 accccgaggc gtggccggtc cagcagatca ccatcggcgg ggtgacctac accaaggccc
      721 aggccatcta ctggatggac atggcgagcc gcggcgacaa gacgatcgac ctgttcaagc
      781 agctcgtcgc cacccggctc aacgtgatcc tcggcaacga cgcgagctgc gtcagctcgt
      841 cgatgtacaa cgccggctcc tggctggcca ccaacccgct ggggagcaac gtgaggtcga
      901 acagcgccgc ctggctgacc ggcgggccgc tgcaccagag gttggacgac tacaacaacg
      961 ggaggctctg cgcgccgcac aggggctgat ccggagcacg cgacgggaag gccggccttc
     1021 gccttcccgt cctcttccgg ctccactcgc gaagaatcgt ccgagcatct ataatatttt
     1081 ctagagtaga tcaacggtca tacaaccaag aaggcttcgg gtgcggaaga tgggcgagga
     1141 gaaacagctc ggcagggagt gcgcgtcagg agggcaggag ctgctcgtct ccagggaggg
     1201 gaaccgtctc gaggggcccc ggccctaccg gcggccggat ctcgtggtct acggcaggct
     1261 ctcagagctg acccgtctcg acggtaatgt ggaatttgat ggcattgccg gatcccagac
     1321 tgtaacctga cgaaccctcg gctagagagc cccccttctt cacaggaaaa gtcctgcctt
     1381 gatcacctgg gagcgcatcc cggggacggc gccacggccc atcgcctcct gcccctctcc
     1441 gacctccggc ggcctccgtt gtctgtggca cggagggttc atcgccaacc ggcgggacct
     1501 agagctcgag accggcttcg ccggcgcctc ggacgccgac ctcctcctcg aaatctatcg
     1561 ccgcgagggc ctgcagacac cgcgacgcat cgccggcacc tttgcctgga tcctttggga
     1621 cggcgacgct cagcgtctcc tggcagcccg ggaccgcctg ggcacgcggg gcctcttctt
     1681 ccgcctcttt tcccgctcga tctgtctcgc tgagcgcgtc ggtgccctgc ggcgagagcc
     1741 gccgagcccg gacctcaacc cggtggccgt ggtctgccac atccaggggg agactccgcc
     1801 tcccggggag accttcctga ccggtattca cgccgtcgag ccgggcaccg tgctcgtcgt
     1861 cgagccggag cgactctctt cccagttcta ctggcgtccc gctccccatc catctctcgg
     1921 gtttccgagc gacgaggcct acgccgacgc ctgtcgagag atcctcttcc gggtcaccgc
     1981 cgagtacgcc gggccaacgg ctggaatcac cctcagcggc gggctcgatt ccaccgccgt
     2041 cgccgccgcc ctgcgcgccg cccgaccgga ggacgagttg agggcggtgt catggatctc
     2101 gcccgaactg cccgaggccg atgagagccg ggaaatagag gcggtcgttg cccgcctgcg
     2161 cctgactctc atccctgtcg ccgccgacca gcactggccg ctcagtgagg gtatcgatgc
     2221 gataccggac acgccgcact acaacttcta cacaactctc tgggacgcca ccttccggac
     2281 tgcccgggag cacggcgtga ccactctctt ctcgggaatg agcggcgacc acctgttcgg
     2341 cggtggactc cccccctatt ccgaccttct cctccggggc cgtgtggggc gcatggccgc
     2401 ggagatccgg gccgggctcc gtcaggcccc cggactgccg cggctgctgc gctcccgggt
     2461 gctgggtccc atcgcgcgga gcctgacccc gtggagagac gagtaccgac cacctccggt
     2521 gagctggctc ggtgaggcct ctcaagacct agtacggcag acgaggcccg agctgccgca
     2581 tctcctgatg cccggccggc gcgagcgcct ccgtattctc cgagatcccc tcttgccggc
     2641 ggtcgcgctc ctgatcgagc accaagcgtc gaggcacggc atcgagctcc gccaccccct
     2701 gctggaccat cgcctgttcg acttcgcggc cgcgctgccg acggagcaga cctggtctgg
     2761 cgggctcaag aaggcgatcc ttcgccgagc actaggggac agcctgcccc gagaggtgac
     2821 agagcgcccg aggaaggttt atccagtggc gatcttccat cgcgggttgc gtgagcgtga
     2881 gcaatccaag gcctgggcgc tgatgacgga catgcgcgcc gccgagatgg gcttcgtcga
     2941 cgagccgaag ctgcgcgatg agtaccggcg ctacctgtcg ggggagacgc agagagccct
     3001 cttctggcac actctgaccc tcgaagcctg gttgagacgg tactttcccc gatgatcgcc
     3061 ccggaagagc acccggacac gtcgcaggag cgcctgcgcc agctcgccgc gagcctgcgg
     3121 gagctccggg gaacgctgcg gcgggtcgac tatcgccaag ccgaggcgga ctgcgagctg
     3181 ctggccacgg ctctcctgca agagattcca cgcgcggagc tggatcggtt ccgcttcgcg
     3241 gcgatccccc gcggcggcct gatcgtcctc ggcatgctgg cctatgccct cgatctaaac
     3301 cccgaacacc tccagcctca cgcaggagat ggcgcgctcg tcctggtcga cgactgcgct
     3361 ctgaccggcg ctcggttcgc ctcccatctg gcgaagacac aggcacggga agtggtgttc
     3421 gtccatctct actcccaccc ggctctccgc caagccatcc gcgagcagga ggagcgggta
     3481 cgggcctgcc tggcggcgca cgacctgcgg gatacgcgcg gcgtcgccga tcctgagtgg
     3541 caggagagat ggcaccggcg gctgggaggg cagcgctact ggtacggcct gcccgagctg
     3601 gtctgcttcg cctggaacga gccggaccgc cctttctgga acgccgccgc ggaacgggtg
     3661 gaggacggat ggaaggtcat ccccccgcac ctctgcctca agacacggat cgcgctcgcc
     3721 ggcccctgtc cggtcccggc ccgacgtact tggcagtcgc cggcggacgt ggtcagcggc
     3781 gagttcgacg ggaccctttg gctttgccag acgacgaccg accgcgtcta ttcgctggac
     3841 ggagtgggcg ccgagatctg gcgcaccctg gcctcttg
//
LOCUS       DASPJI010000252         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_646189, whole genome shotgun sequence.
ACCESSION   DASPJI010000252 DASPJI010000000
VERSION     DASPJI010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646189"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2163)
                     /locus_tag="VEL74_06600"
     CDS             complement(<1..>2163)
                     /locus_tag="VEL74_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG62232.1"
                     /translation="GGLDMPALAATLMEIVRRHESLRTVFAVHPDHGEPVQRVLPAAE
                     GWRQPLPLIDLSGLGSLAEGEARRLAIEEAARPFDLEKGPLLRTALVRLAARDHLLLA
                     NMHHIVSDGWSLGVMVRELEALYATAREGRPSPLPELPVQYPDFAVWQRRWLSGEVLE
                     RHLDYWRGRLAGAPALDLPLDRPRPAVPSFRGAARQLGFGAELSQGIGLLGRRHGATP
                     FMVLAAGLFTVLARYSGQPDISLGSPIANRNRSETEGLIGFFVNTLVLRADVGRAESF
                     KELLEQVREVTLGAYAHQDMPFEKLVGELLPERDPSRTPFFQVSLAVQNAPLSAARLA
                     DLDLLPVDLPGSTAKFDLTLAVGEVGGGLAGSVEYATDLYDGSTALRLCRHWLRLLEA
                     AVEDPAGSPVALSLFSAAERHQLLVEWNDTVRALPDATVVDLFREQAARRPGAIALSS
                     REGDLSYADLDARSDRLARRLADLGVGAEMPVGLLAERSLALLVGMLGILKAGGAYLP
                     LDPNYPAARLAWMLTDSGARVLLGDPDKVASLPEEARPEIVVELTADPEDAAGPDPGA
                     VLPGNLAYVLYTSGSTGRPKAVGITHGNIVRLVREGGYAHLGPDEVHFQFAPVTFDPS
                     TIEIWPALTHGGRLVLAPPGLLSLGDLGRTIEGQGVTTLQLVTGPFNQMVDHQLDSLR
                     GVRQLLTGGEAMSPVHAERLLTGLPGVALTNLYGPTEVT"
BASE COUNT          339 a          812 c          745 g          267 t
ORIGIN      
        1 cgtcacctcg gtcggtccat agaggttggt cagcgccacc cccggcaggc cggtcaagag
       61 ccgctcggcg tgcaccggcg acatcgcttc gccgccggtc aggagctggc gaaccccccg
      121 caaggagtcc agttggtggt ccaccatctg attgaaggga cccgtgacca gttgcagcgt
      181 ggttactcct tggccttcga tcgtccgtcc caggtcgccg agcgacagca ggcccggcgg
      241 cgccaggacc agacggccgc cgtgagtcag cgccggccag atctcgatgg tcgacgggtc
      301 gaaggtgacg ggagcgaact ggaagtggac ctcgtccggc ccgagatggg catagccacc
      361 ctcacgcacc agccgcacga tgttgccgtg ggtaatcccc accgccttgg gccgccccgt
      421 cgatcccgag gtgtacagga cgtacgcgag gttgccgggc aggacggcgc cgggatcggg
      481 tccggcggca tcctccgggt ccgccgtcag ctccaccacg atctccggcc gggcctcctc
      541 gggcagggag gccaccttgt ccggatcgcc cagcaggacc cgggctccgg agtcggtcaa
      601 catccaggcg agccgcgccg ccggatagtt ggggtcaagg ggcagatagg cgccgccggc
      661 cttgaggatg cccagcatcc ccaccagcaa cgcgagggag cgctcggcga gcagacctac
      721 cggcatctcg gcgcccaccc ccaggtccgc caaccgccgc gccagccggt ccgagcgggc
      781 atccaggtcg gcataggaga ggtccccctc gcgggacgaa agggcgatcg cccccggccg
      841 gcgggcagcc tgctcgcgga agaggtccac tacagtcgca tcgggcagcg cccgcacggt
      901 gtcgttccac tccaccagga gctggtggcg ctccgccgcc gagaacagag agagggcgac
      961 aggagagccg gcggggtctt ccaccgccgc ttccagcagg cgcagccagt gccggcacag
     1021 gcgcagggcc gtggacccgt catagagatc ggtcgcgtac tccacgctgc cggccaggcc
     1081 gccccccacc tcgcccaccg ccagggtcag atcgaacttc gccgtcgagc ccggcaggtc
     1141 gacggggagg aggtccaggt ccgccaggcg tgccgcggac agcggcgcat tctggaccgc
     1201 caacgagacc tggaagaacg gggtccgcga cgggtcccgc tccggcagca gctctccgac
     1261 cagcttctcg aacggcatgt cctggtgggc gtaggcgccc agcgtgacct cccgcacctg
     1321 ctccaggagc tccttgaagc tttcggcccg ccccacgtcc gcccgcaaca ccagggtgtt
     1381 gacgaagaag ccgatcaacc cctcggtctc gctgcggttg cggttggcga tgggtgagcc
     1441 cagggagatg tcgggctggc cgctgtagcg cgccaagacc gtgaagagcc cggccgccag
     1501 aaccatgaac ggggtggccc cgtggcgccg ccccagtaag cctatcccct ggctcagctc
     1561 cgcgccgaag ccgagctgcc gcgctgcccc gcggaagctc ggcaccgccg gccgcggccg
     1621 gtccaacggc aggtccaggg ccggcgcgcc cgccagccgg ccgcgccagt agtccaggtg
     1681 gcgctccagc acctcgccgg agagccaccg ccgctgccag acggcgaagt cggggtactg
     1741 gaccggcagt tccggcaacg gcgacggccg cccctcccgg gcggtggcat agagcgcctc
     1801 cagctcccgc accatcacgc ccagcgacca gccgtccgaa acgatgtggt gcatgttggc
     1861 cagcagcagg tggtcccgcg cggccagccg caccagcgcc gtgcgcagca gcggcccctt
     1921 ctccaggtcg aacggccgcg ccgcctcctc gatcgcgagg cggcgcgcct cgccctccgc
     1981 cagggagccg agccccgaga ggtcgatcaa cggcagcggc tgccgccagc cctcggcggc
     2041 gggcaggacc ctctgcaccg gctcgccatg gtccgggtgg acggcgaaga ccgtgcgcag
     2101 gctctcgtga cgccgcacga tctccatcag tgtcgcggcc agcgccggca tgtccagccc
     2161 gcc
//
LOCUS       DASPJI010000253         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_477734, whole genome shotgun sequence.
ACCESSION   DASPJI010000253 DASPJI010000000
VERSION     DASPJI010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477734"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VEL74_06605"
     CDS             <1..884
                     /locus_tag="VEL74_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62233.1"
                     /translation="EPMLRVPYDLPTFDQPVAVLDAAGGGHLVVARQGSIEDTGIPEE
                     VCERFDGLFAWSFDAAPAGLLLQERFWVEVGWVGSPGQSGVGRPVVLTPDSGYFWFFG
                     PDNVELMLKVLDGRSLNGHFWVFYASLSDVAYTITVTDTATGRVRAYDNASGQLASRG
                     DTAAFPAAGASVAVPGSLLPGGPSSGLLLGDRFEVEVAWTNPETGISGIGYGVKLTDD
                     SGYLWFFGPNNIELVVKVLDGRAVNGRYWVFYGALSNVEYTVRVRDTATGQVREYHNP
                     PGQLRSHADTAAFPLPE"
     gene            complement(1301..>2309)
                     /locus_tag="VEL74_06610"
     CDS             complement(1301..>2309)
                     /locus_tag="VEL74_06610"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HYG62234.1"
                     /translation="SQRLTDRHIDLPRLREGGVDALVFALFIPASLSPEEGWELTLKL
                     HQITLDHLVPGMEQVGTEQQVREAAARGAVGVILGLENGRPLSVPGALAHCARIGVRY
                     VTLTHWASHEWCDASTDEPRHGGLSEEGVKIVREMNRLGVLPDLSHISDDAVRHVLDV
                     SKAPPLASHSSSRALCDHPRNLSDDLVKEIAERDGLVMANTYPAFVGQQASRVEKERM
                     ARLKPAFEEMDAAYLREPGKHWRERERLLKDNPLPPVPLSVFVDHVIRLVELGGEEHV
                     GIGTDLDGISEPLEGFEDVSRFPNLTAALLERGVDKAGVRLILGENFLRVLRRAEQLA
                     V"
BASE COUNT          331 a          764 c          836 g          378 t
ORIGIN      
        1 tggagccgat gctgcgggtc ccctacgatc tgcccacctt cgatcagccg gtggccgtgc
       61 tcgacgccgc cggcggcggg cacctcgtcg tcgcccgcca gggctcgatc gaggataccg
      121 ggatcccgga ggaggtctgc gaaaggttcg acggcctctt cgcctggagc ttcgacgccg
      181 ccccggccgg tctcctgttg caggagaggt tctgggtcga agtcgggtgg gtcggctccc
      241 ccggccagtc cggagtcgga cgtccggtgg tgctcacccc ggactccggc tacttctggt
      301 tcttcggccc ggacaacgtg gagctgatgc tcaaggtcct ggacggccgg agcctcaacg
      361 gccacttctg ggtcttctat gcgtcgctgt cggacgtggc ctacacgatc acggtgaccg
      421 acacggcgac ggggagggtg agggcctatg acaacgcctc cggccagctc gccagccggg
      481 gggacaccgc cgccttcccg gcggccggcg cctcggtggc ggtgcccggt tctctgctcc
      541 ccggaggtcc cagctcgggt ctcctcctcg gcgaccgttt cgaggtggag gtcgcctgga
      601 ccaacccgga gaccggcatc tccggcatcg gctacggggt gaagctgacc gacgacagcg
      661 gctatctctg gttcttcggg ccgaacaaca tcgagctggt ggtcaaggtg ctggacggcc
      721 gcgcggtcaa cggccgctac tgggtcttct acggcgccct gagcaacgtg gagtacaccg
      781 tccgcgtccg cgacaccgcg accgggcagg tccgggagta ccacaacccc cccggccagc
      841 tccgcagcca cgcggacacg gcggcgttcc cgctgccgga gtagggggcg ttacccccgc
      901 tgttcatcag cggcgggggg cgtctttccc ggctcctgcg ccgcaagcgc tttcgagtcg
      961 cggcggagcg ttcgaccttg cggcgaagcg ttccaagtcg cggcagggga tctcaactcg
     1021 cggtgaagcg ttcgagcttg cggcgaagcg ttcgactttg cggtgaagac ttcaatcttg
     1081 cggtgaatcg attgaagtcg cggcgaatct tttgatcttg cggtgaacga aacgaagtcg
     1141 cggtgaatct ttccgagttg cggtgaagag tctcgagtcg cggtgaacga ggagaggtcg
     1201 cggcgaaggt tctggagtcg cggcggagga aacggggtcg cggcgacggg tttggagtcg
     1261 cgacgccgcg aaaggcgtcg cgactcctcg gatgcggtgt ctacaccgcg agctgctcgg
     1321 cccgccgcag cacccgcagg aagttctcgc ccaggatcag acggacgccg gccttgtcga
     1381 cgccccgttc gaggagcgcc gcggtcagat tcgggaagcg ggagacgtcc tcgaaccctt
     1441 ccagcggctc cgagatgccg tcgaggtcgg tgccgatccc cacgtgctcc tcgccgccga
     1501 gctcgaccag acggatgacg tggtcgacga agaccgacag cggcacgggg ggcagcgggt
     1561 tgtccttgag caggcgctcc cgctcgcgcc agtgcttgcc gggctcgcgc aggtaggccg
     1621 cgtccatctc ctcgaaggcg ggcttgagcc gagccatccg ctccttctcc acccggctcg
     1681 cctgctgccc gacgaaggcg gggtaggtgt tggccatcac caggccgtcg cgctcggcga
     1741 tctccttgac caagtcatcc gacaggttgc gcgggtggtc gcagagggcg cgggacgagc
     1801 tgtggctggc gagcggcggg gccttggaga cgtccagcac gtggcggacg gcgtcgtccg
     1861 agatgtggga gaggtcgggg aggacgccca ggcggttcat ctcgcggacg atcttgaccc
     1921 cttcctccga cagaccgccg tggcgcggct cgtcggtgga ggcgtcgcac cactcgtggc
     1981 tcgcccagtg ggtgagggtg acgtagcgga cgccgatgcg ggcgcagtgg gcgagcgctc
     2041 cgggcaccga caggggccgg ccgttctcca ggcccaggat gacgccgacc gcgccgcgcg
     2101 ccgccgcctc ccggacctgc tgttcggtgc ccacctgctc catgcccggc accagatggt
     2161 ccagggtgat ctggtggagc ttgagggtca gctcccaccc ctcctcgggg ctcagggagg
     2221 cggggatgaa gagcgcgaag accagggcgt cgacgccccc ctcgcgcagc cgcggcaggt
     2281 cgatgtggcg gtccgtcagc cgctgggac
//
LOCUS       DASPJI010000254         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_646223, whole genome shotgun sequence.
ACCESSION   DASPJI010000254 DASPJI010000000
VERSION     DASPJI010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646223"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VEL74_06615"
     CDS             complement(<1..821)
                     /locus_tag="VEL74_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HYG62235.1"
                     /translation="MNINDLLKIAVERKASDLHLKVGSHPVIRVDGDLMTLPELKRLM
                     QEDTIAMAFSIMNARQKQRFKEEFEIDISYSVPGLGRFRCNVFQQRGAVGLVLRVIPA
                     RIQSVRELMLPPVLERICEERRGLILCTGTTGSGKSTTLAAMIDSINSARTEHIMTIE
                     DPIEFLHRDKKALVNQREIDVDTRTFANALRSALRQDPDVILVGEMRDMETIETALHA
                     AETGHLVFSTLHTLDATETINRIISVFPPHQQKQIRLQLAAVLKAAIAQRLIPSA"
     gene            complement(863..1846)
                     /locus_tag="VEL74_06620"
     CDS             complement(863..1846)
                     /locus_tag="VEL74_06620"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HYG62236.1"
                     /translation="MSNPFANVRWIWKNGQLVEFEKATVHAMSHALHYGSGVFEGIRC
                     YKTRRGPAVFRLAEHLDRLEYSARIYRMPMPFSRDELAEAILETIRANEMESCYIRPV
                     VYRGFGTMGVNPLKSPVEAMVAVWSWGKYLGEGATEGVDVGVSTWRRPASDALPAIAK
                     STGGYLNSQLIKMEALSNGFVEGIALDAQGFVSEGSGENLFLVRRGTLVSPSLAASVL
                     DGITRDAVITMAREMGIPVREELVPRALLYTCDELFMTGTAAEITPIRSVDHYAVRTC
                     PGEITRRLLDAFMAAVTGEADDRHGWLTPVHAAREAGTAPPPPAPALAREA"
     gene            complement(1911..>2397)
                     /locus_tag="VEL74_06625"
     CDS             complement(1911..>2397)
                     /locus_tag="VEL74_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HYG62237.1"
                     /translation="LLVTTLRPERASWMVEKATELGVSAIRFLNSARAPRELGGGTIE
                     RMRRVAGAAVEQCHRSRVPDITGPHGWKEVGLLAEHAQGRWWLDTAAAEEGWGEIPGA
                     SGNGSGVLLVGPEGGWSPEERDELLAGGMGDWRPVGLGPRVLRAETAAVVGTAFLMLG
                     R"
BASE COUNT          349 a          796 c          878 g          374 t
ORIGIN      
        1 tccgcgctcg ggatcagacg ctgcgcgatt gctgctttca acacggccgc gagctgaagc
       61 cgaatctgct tctgctggtg cggcgggaag accgagatga tgcggttgat cgtttcggtc
      121 gcgtcgagcg tgtgcagcgt cgagaacacg aggtgtccgg tctcggccgc atgcagcgct
      181 gtttcgatgg tttccatgtc gcgcatttcg ccgacgagga tgacgtcggg gtcctggcgc
      241 agggcggagc gcagggcgtt ggcgaacgtc cgcgtgtcga cgtcgatctc gcgctggttg
      301 accagggcct tcttgtcccg gtggaggaac tcgatcgggt cctcgatggt catgatgtgc
      361 tcggtgcgcg ccgagttgat ggaatcgatc atcgcggcga gcgtcgtcga cttgcccgag
      421 ccggtggtgc cggtgcagag gatcaggccg cggcgctcct cgcagatgcg ctcgaggacc
      481 ggcggcagca tcagctcgcg gacgctctgg atgcgcgccg ggatgacgcg cagcaccagg
      541 ccgacggcgc cgcgctgctg gaagacgttg caacggaagc ggccgagccc gggcaccgag
      601 tacgagatgt cgatctcgaa ctcctccttg aagcgctgct tctggcgcgc gttcatgatc
      661 gagaacgcca tggcgatggt gtcctcctgc atgaggcgct tgagctcggg cagcgtcatc
      721 aggtcgccgt cgacgcggat caccgggtgg ctccccacct tgaggtggag gtcggaggcc
      781 ttccgctcca ccgcgatctt gagcaggtca ttgatgttca tcgtgggcaa gctccctggt
      841 tacgaatgat cggatcgtcg cgctaggcct cgcgcgccag cgccggagcc ggcggaggag
      901 gcgcggtgcc ggcctcccgc gccgcgtgga ccggcgtcag ccagccgtgg cggtcgtcgg
      961 cctcgccggt gacggcggcc atgaacgcgt cgagcaggcg gcgggtgatc tcccccgggc
     1021 aggtgcggac ggcgtagtgg tcgacggaac ggatgggggt gatctcggcc gcggtgccgg
     1081 tcatgaacag ctcgtcgcag gtgtagagca gcgcccgcgg caccagctcc tcgcgcaccg
     1141 ggatgcccat ctcgcgcgcc atggtgatga ccgcgtcgcg ggtgatgccg tcgagcacgg
     1201 aggcggcgag cgacgggctg accagggtgc cgcggcggac caggaacagg ttctcgcccg
     1261 acccttcgct gacgaagccc tgggcgtcga gggcgatccc ctcgacgaag ccgttcgaca
     1321 gcgcctccat cttgatgagc tgcgagttga ggtagccgcc ggtcgacttg gcgatggcgg
     1381 gcagcgcgtc ggaggcgggg cggcgccagg tggagacgcc gacgtcgacc ccttcggtcg
     1441 ccccctcgcc caggtacttg ccccaggacc agaccgccac catcgcctcg accggcgact
     1501 tcagcgggtt gacccccatg gtgccgaagc cgcggtagac gaccgggcgg atgtagcagg
     1561 actccatctc gttggcgcgg atggtctcga ggatcgcctc ggccagctcg tcgcgggaga
     1621 acggcatggg catgcggtag atccgggccg agtactccag ccggtccaga tgctccgcca
     1681 gccggaagac cgccgggccg cgccgcgtct tgtagcagcg gatcccctcg aacacgccgg
     1741 agccgtagtg gagcgcgtgg ctcatggcgt gcacggtggc cttctcgaac tccacgagct
     1801 ggccgttctt ccagatccaa cggacgttgg cgaagggatt ggacaaaaag acctcctttg
     1861 agcgcaagcc gagatgttac ggggatgcta gcccgaatgg ggcggggtgt ctacctcccg
     1921 agcatcagga aggccgtgcc caccaccgcc gcggtctcgg cgcgcaggac gcgcgggccc
     1981 agcccgacgg gccgccagtc gcccatgccg ccggccagga gctcgtcgcg ctcctcgggc
     2041 gaccagcccc cctcggggcc gacgagcagg acgccggagc cgttgccgga ggcgcctggg
     2101 atctcccccc acccctcctc cgcggcggcg gtgtcgagcc accagcggcc ctgggcgtgc
     2161 tcggcgagga gcccgacctc tttccagccg tgggggccgg tgatgtccgg gacgcgcgag
     2221 cggtggcact gctcgacggc ggcgccggcc acccggcgca tgcgctcgat ggtgccgccg
     2281 ccgagctcgc gcggcgcccg ggcgctgttc aggaagcgga tggccgagac ccccagctcg
     2341 gtggccttct ccaccatcca ggaggcgcgc tcggggcgca gcgtggtcac cagcagc
//
LOCUS       DASPJI010000255         7798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_820496, whole genome shotgun sequence.
ACCESSION   DASPJI010000255 DASPJI010000000
VERSION     DASPJI010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7798)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820496"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VEL74_06630"
     CDS             <1..306
                     /locus_tag="VEL74_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62238.1"
                     /translation="QSVLLVGREAAKAGDVRIVWVGAPGDAFGLRVDFWVVNGGGEEL
                     TVGAFLRTGEGQTTITLPLDGDGRLRYRYELRRLGPQGEELVRLGEGESNLLVVQAA"
     gene            complement(1169..1513)
                     /locus_tag="VEL74_06635"
     CDS             complement(1169..1513)
                     /locus_tag="VEL74_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62239.1"
                     /translation="MPSHSDRTPSGVDLSRPTESTRRHLYDLVVRIHFNPTPEELQQA
                     KEDPGNHWIPDYGPDAAELTVWYDRGRWYAAWQSLEEEPDPRAPWRRWSVVRIVEDAD
                     PSSATGVTFLEV"
     gene            complement(2160..2714)
                     /gene="pyrE"
                     /locus_tag="VEL74_06640"
                     /pseudo
     CDS             complement(2160..2714)
                     /gene="pyrE"
                     /locus_tag="VEL74_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943204.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(2740..4125)
                     /locus_tag="VEL74_06645"
     CDS             complement(2740..4125)
                     /locus_tag="VEL74_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYG62240.1"
                     /translation="MARSNDSFRASVLVVDDEAAIRDSLHMILEYEGYRVDEASGGSQ
                     ALAKVADRAPDAVLMDIKMPEMDGLDVLKAFRERGYDMPVLMISGHADVQTAVEATRR
                     GAYDFFEKPLQRERVLLALRNAIESYRLQTENRVLRQEPDLLIGSAPAMKRLRETIER
                     AAPTPATVLITGESGTGKELVARAIHRQSTRRDKTLVQVNCAAIPEELIESELFGHEK
                     GSFTGAVRRQMGKFVTADGGTIFLDEIGDMSARTQAKVLRVLQNGEVEPVGAERTVNV
                     DVRVVAATNRDLEEEIKAGRFREDLFYRLNVIPVRTPSLREHLEDLPQMVDYFVRRYA
                     EQNNYRAKEFTPEALGHLQALPWKGNVRELRNLVERLLILSPGEKVTREDVVAATGGT
                     RPELSSAVLAVKTLREFRDVSERMFLLHKLEENNWNVTQTAQAIDTPRSNLYKKLDQY
                     EIRREGQLNET"
     gene            complement(4269..5831)
                     /locus_tag="VEL74_06650"
     CDS             complement(4269..5831)
                     /locus_tag="VEL74_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYG62241.1"
                     /translation="MSTTRHRNITTFLLVLGAVVFGMVLAGGLQLTVPSDAQPQPAAT
                     LSATQLAAEHGEIAALPSFADLAEAVDPAVVSIQAATIERGRRGRGVDPFEFFFGPRQ
                     RPDERNPNDPGQTPGAPGGPGGEEFRSDSGGSGFVISRDGLVVTNYHVIEGATRVTVH
                     LGDRDYPAEVKGTDQATDLALLKINPGRDLRYLELGDSEGVRVGDWIMVVGNPLNLDK
                     TVTTGVVSAKGRSIGINDISFENFIQTDAAINFGNSGGPILNMRGQVVGIATAINYGA
                     ENIGFAVPVNTLKEILPQLRDKGKVSRGYLGIEVANLDYTTAQSFGLETTEGALVQSV
                     VNNQPADEAGIRHGDIIVSVDNVQVKTTRDLINYVSNKGPGVTVNVALLREGKRMERK
                     VRLSERPDQNQGDEEEESEQGESGIDWLGIQYQGLTPSNRNSLGLPENARGVLVTQVE
                     PTSPLYDQSVRPGGGIQFVITEVSGREVSSVADFESIVRGAKSGSFLRFYLQTYRRGE
                     PLQAFFAVVQVP"
     gene            6211..6789
                     /gene="rimP"
                     /locus_tag="VEL74_06655"
     CDS             6211..6789
                     /gene="rimP"
                     /locus_tag="VEL74_06655"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HYG62242.1"
                     /translation="MAEKKVADRAGAKSAGAKQSGDRKPAAPRVLDPKLRQEIGDIAD
                     AAGCELVHAEIKGGTLRLILDKEEGGVNLSDCEYVSKQVSAYLDVVDFGKSRYVLEVS
                     SPGLDRQLYQPRDYDRFAGRLARVTIEEGGTKKTVVARLQGLRRQDGQPPEQPGEAAA
                     AEAEVVLLDDKSGESWAVPLKNIRLARLEIEL"
     gene            6812..>7798
                     /gene="nusA"
                     /locus_tag="VEL74_06660"
     CDS             6812..>7798
                     /gene="nusA"
                     /locus_tag="VEL74_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HYG62243.1"
                     /translation="MPQAQKAQKAQAAQDVNFNEILRQVSREKDIDLDRWVAALEDAM
                     ASAAKKQHRIKEPVRAHLDRDTGRFDAFIVKKVVETVEDPLAEWTVEEARDHKDGANV
                     GDEIHLPISTDGLGRIAAQSAKQVLYQRVREAERENIYNEFIDRVGEVLNGTVKRFER
                     GDIIIDLGRTEAVVPRSEQARHERYSQGERIRAVIVEVHKQPKGPQIVLSRTDPRLLV
                     KLFETEVPEIYDGTVVIKNAVRAPGERAKVAVYSRERDVDPVGACVGMKGSRVQSIIR
                     ELRGEKIDIIEYSDDLVTFAQSALAPAKITRVSVTHHGEVPHLDVIVEDEQLS"
BASE COUNT         1185 a         2706 c         2675 g         1232 t
ORIGIN      
        1 cagtcggtgc tgctggtcgg ccgcgaggcg gcgaaggccg gggacgtgcg catcgtctgg
       61 gtgggagcgc cgggggacgc cttcggcctc cgcgtcgact tctgggtggt gaacggcggc
      121 ggcgaggagc tgaccgtcgg cgccttcttg cgcaccggcg agggccagac gacgatcacc
      181 ctccccctcg acggcgacgg ccgcctccgc taccggtacg agctgcggcg cctgggccct
      241 cagggggagg agctggtacg gctgggcgag ggcgagagca acctcctggt cgtgcaggcg
      301 gcgtgacggt tcgccttccc gggcgccatg gatgccgtcc agacgtcccg gatctcgtcc
      361 cagcgccctg gatgccatcc agacgttccg gattccatcc agacgcagcg ggaaacatcc
      421 gggagcctcg gatgccatcc ggaagcgccg gacggctccc gaacgtcccg gacggcgtcc
      481 aattcaaccg gatggattcc ggacgtgccg ggagcttccc ggacgcttcg gatcgcgtcc
      541 aattcaaacg gatggattcc gggggagccg gatgtgtacg gtgtcgggcg ggggcttcct
      601 cccccctggg cggtgggcgg cagggagcgg gggaggaccg aggtgggggg ttgcctttgc
      661 gcgggccggg ggagaaaaaa gaccccccct ccttcacctc ccccgctccc tgccgcccac
      721 cctcccaggg ggggaggcac caccaccccc tgcggccccg aagcttccga tgcgctgccg
      781 aagcttccga tgcgccgccg aagcttccaa ctccctgccg aagcttccaa ggcattgtcg
      841 caatctccgc cgggccgttg gaagcttcca agcgctgccg gagtctccga cgcgccgtcg
      901 tactcttcgg cgcgccgttg gaagcttctc cagaccgttg gaagcttcgg cgagccgtca
      961 gattttccgc caggctgtcg aagtctccga ctggccgttg caaccttccg acgcgccgcc
     1021 gaaatcttcg actcgccgtc ggagtctccg gcaagccgcc ggaagctccg ccaagagaaa
     1081 agcgcctccc ccgcgacggc ggagaaggcg ctgcttcctt ctacagaggg gcgggccgcg
     1141 cgaggcggcg gacatcgcgc caggggcgtc agacttccag aaaggtgacg cccgtcgcgc
     1201 tcgacgggtc cgcgtcctcg acgatccgca ccaccgacca gcggcgccag ggcgcccgcg
     1261 gatccggctc ctcctccagg gattgccagg cggcgtacca ccggccgcgg tcgtaccaga
     1321 ccgtcagttc ggcggcgtcg ggaccgtagt cgggaatcca gtgattcccc gggtcctcct
     1381 tcgcctgctg gagctcctca ggagtgggat tgaagtggat ccgcaccacc aagtcataga
     1441 gatgacggcg ggtggattcg gtgggacggg acagatcgac gcccgaaggc gtacgatcac
     1501 tgtggctggg catgagttct acctcctgtc cggcaaggcc tcggggtgga gtggcaactc
     1561 ccccccttgg ccgcttggac cctggaaccc ccgatgagac acgcgctcac cttggaggca
     1621 cagcggtcct gtacggctgt gctgtgcagc tatcgttacg ttctctcccc ctaccacctc
     1681 ctttgttgta cggcttacga ccgccaccga gcctataccg ggctcggggg cgcgtcaaga
     1741 gggaaatgag agagtcttac gggaaagttg cggaatgcgg agcggagaaa aaccccccac
     1801 ctgacctccc ccactccctg ccgcccgacc gcccaggggg ggaggcacca ccgccccctc
     1861 atccggcggt ccgtgcaggt ccgtgttcgt ccgtgtcggt ccgtgttgtt ggttccgcct
     1921 ccccctctcc cggcgggggc gggcggagat gggagagggg gtcggggggt gaggactccc
     1981 acgcgcagac gcacccgcgg ccgccgcccc ctctcccggg gcggggagga gagggagggg
     2041 cgggagaggg ggccgggggg tgagaggccc tacggccggc ccgtggccgg cgggttcgtc
     2101 gcggccgagc cttcgcccgc ggcggcctcc tgctcccagg aggcggcggc cggctcggga
     2161 cggctgcccg gcttctccgg cggctgggcg ccggcctggc actgcgggca ctctcccggc
     2221 ggattggacg gcaggtccag ctcgatcagc gagcggaacg gcacgtcgaa ggggtcgcgg
     2281 ccggcggtgc gatcgatgat ggcgccgatg gccaccaccc gggcgccgcg ggccgtggcg
     2341 agcgacgccg tctccagcgt cgaacggccg gtggtcacca cgtcctcgat gatcaccacc
     2401 cgctccccct ggcgcagggt gaagccgcgg cgcagcccca tctcctcccc cttgcgctcg
     2461 gtgaagcggt gcgggactcc cagcgccgcc gccacctcgt gaccgatgat cacgccaccc
     2521 agagccggcg acagcaccga gtcgggatgg aacgcgcgga gcttgtccgc caggtcctgc
     2581 cccaccttgc gggcccggac cgggtcctcc agcagcaggg cgcactgcac gtaggcctcc
     2641 gagtggaggc ccgaggagag aaggaagtgc ccccggcgca gcgcgccgct ctcctccagc
     2701 aggcgtagaa ggatcctctc gttctccggc cgatctttgt caagtctcat tgagctgccc
     2761 ctcccggcgg atttcgtact ggtcgagctt cttgtaaagg ttgctccgcg gcgtgtcgat
     2821 ggcctgggcg gtctgggtga cgttccagtt gttctcctcc agcttgtgga gcaggaacat
     2881 gcgctccgag acgtcgcgga actcccgcag cgtcttgacc gccagcacgg cgctggagag
     2941 ctccgggcgc gtgccaccgg tggccgcgac cacgtcctcc cgcgtcacct tctctcccgg
     3001 cgagaggatc agcaaccgct ccaccaggtt cctgagctcc cgcacgttgc ccttccaggg
     3061 cagggcctgg agatggccca gcgcctccgg ggtgaactcc ttggcgcgat agttgttctg
     3121 ctcggcgtag cggcgcacga agtagtccac catctgcggc aggtcctcca ggtgctcgcg
     3181 cagggacggc gtgcgcaccg ggatgacatt cagacggtag aagagatcct cccggaaccg
     3241 tccggccttg atctcctcct ccagatcgcg gttcgtcgcc gccaccaccc gcacgtccac
     3301 gttcaccgtc ctctccgccc ctaccggctc cacctctccg ttctgcaaaa ctcgtagcac
     3361 cttggcctgg gtgcgggcgg acatgtcgcc gatctcgtcg aggaagatgg tgccgccgtc
     3421 ggcggtgacg aacttgccca tctgccgccg caccgcgccg gtgaagctcc ccttctcgtg
     3481 gccgaacagc tccgactcga tcagctcctc ggggatggcg gcgcagttga cctgcaccag
     3541 cgtcttgtcg cggcgggtcg actggcggtg gatggcgcgc gccaccagct ccttgccggt
     3601 gccgctctcg ccggtgatca gcaccgtcgc cggcgtcggc gccgcccgct cgatcgtctc
     3661 gcgcagccgc ttcatggcgg gggcgctgcc gatcagcagg tcgggctcct ggcgcagcac
     3721 ccggttctcg gtctgcaggc gatagctctc gatggcgttg cgcagcgcca gcaggacgcg
     3781 ctcccgctgc aagggcttct cgaagaagtc gtaggcgccg cggcgggtcg cctccaccgc
     3841 cgtctgcacg tcggcgtggc cgctgatcat cagcaccggc atgtcgtagc cgcgctcgcg
     3901 gaaggccttg agcacgtcca gcccgtccat ctccggcatc ttgatgtcca tcagcaccgc
     3961 gtcgggcgcg cggtcggcca ccttggcgag agcctgggag ccgcccgacg cctcgtcgac
     4021 ccggtagccc tcgtattcga ggatcatgtg caggctgtcg cggatcgcgg cctcgtcgtc
     4081 caccaccagc acggaggcgc gaaagctgtc attgcttctg gccatggtcg tccaagtgta
     4141 acaagaaagc ggccggtgct ccccgccgcg gcccgcctct cctgggagag agggggccgc
     4201 ggcggggagc accggcctgc tgggtagcaa gagttggcac ggggccggcg ggccggcccc
     4261 gtcccggctt acggtacctg caccaccgcg aagaacgcct gcagcggctc gccgcgccgg
     4321 tacgtctgca ggtagaagcg gaggaacgag cccgacttgg cgccgcggac gatcgactcg
     4381 aagtcggcga cgctcgagac ctcgcggccg ctgacctcgg tgatcacgaa ctggatgccg
     4441 ccgcccggcc gcacgctctg gtcgtacagc gggctcgtcg gctccacctg cgtcaccagg
     4501 acgccgcggg cgttctcggg caggccgagg ctgttgcggt tcgacggcgt cagcccctgg
     4561 tactggatgc ccagccagtc gatgccgctc tcaccctgct cgctctcctc ttcctcgtcg
     4621 ccctggttct ggtcggggcg ctcggacagc ctcaccttgc gctccatccg cttgccctcg
     4681 cgcaggagag cgacgttcac cgtcacgccg gggcccttgt tggagacata gttgatgagg
     4741 tcccgcgtcg tcttcacctg gacgttgtcc accgacacga tgatgtcgcc gtgccggatg
     4801 ccggcctcgt ccgccggctg gttgttgacc accgactgca ccagcgcgcc ttccgtggtc
     4861 tcgaggccga agctctgcgc cgtggtgtag tcgaggttgg cgacctcgat gcccaggtag
     4921 ccgcggctca ccttcccctt gtcgcggagc tgcggcagga tctccttgag ggtgttgacc
     4981 ggcaccgcga agccgatgtt ctcggcgccg tagttgatcg cggtggcgat gccgacgacc
     5041 tgccccctca tgttgaggat ggggccgccg gagttgccga agttgatcgc cgcgtccgtc
     5101 tggatgaagt tttcgaacga gatgtcgttg atgccgatcg accggccctt ggcgctgacc
     5161 acgccggtgg tcaccgtctt gtccaggttc agcgggttgc cgacgaccat gatccagtcg
     5221 ccgacccgca ccccctcgct gtccccgagc tcgaggtagc gcaggtcgcg gccgggattg
     5281 atcttgagca gggcgaggtc ggtcgcctgg tcggtcccct tgacctcggc gggatagtcc
     5341 cggtctccga gatggaccgt cacccgggtg gcgccctcga tgacgtgata gttggtcacc
     5401 accaggccgt cgcggctgat gacgaagccg ctgccgcccg aatccgagcg gaactcctcg
     5461 ccgccggggc cgccgggggc gccgggggtc tgccccgggt cgttggggtt gcgctcgtcc
     5521 ggccgctggc gcgggccgaa gaagaactcg aacgggtcca cgccccgtcc gcgccgtccg
     5581 cgctcgatgg tggcggcctg gatcgacacc accgccgggt ccaccgcctc ggcgaggtcg
     5641 gcgaagctgg gcagggccgc gatctccccg tgctcggcgg ccagttgggt cgccgacagg
     5701 gtcgccgcgg gctgcggctg cgcgtcgctg gggacggtca gctgcaggcc gccggcgagg
     5761 accatgccga acaccacggc ccccaggacc agcagaaacg tcgtgatatt gcgatgccga
     5821 gtcgtactca ttgtgcgctc cggtctcctt tccttctcca tgcctacgca aagcaagggg
     5881 cttgccaaga aaatttccct cggagaggcc cggctcgcgc cgctgtctct gccaaattgg
     5941 cacgcccgtg aggcccacgg gacgacattg gggagcggca tgcgcaggcg ggaatttccg
     6001 gcctcctgcc gctgtcttgt agagtgcgcg tgccgtcccc ggatgggaaa tccgccccca
     6061 cgcgttcctc gcggcatctt ccacgagttg aaccagcagg ccggctcttc tataatgaag
     6121 gaagccagct tgcccgaccg gctcccccgg tcggaaagga gtgggtgtga acccactttt
     6181 tttgtttggc gggtctggca tcaagaggac atggccgaga agaaggtagc cgacagggcc
     6241 ggcgcgaaga gcgctggcgc gaagcagtct ggagacagga agcccgccgc tccccgggtc
     6301 ctcgacccga agctccggca ggagatcggc gacatcgccg acgcggccgg atgcgagctg
     6361 gtgcacgccg agatcaaggg cggcaccctg cggctgatcc tcgataagga ggagggcggg
     6421 gtgaacctgt ccgactgcga gtacgtctcc aagcaggtct cggcctacct cgacgtggtc
     6481 gatttcggca agagccgcta cgtgctggag gtcagctccc ccggcctcga ccggcagctc
     6541 taccagccgc gcgactacga ccggttcgcg ggacgcctcg cgcgggtcac catcgaggag
     6601 ggtgggacca agaagacggt ggtggcgcgc ctccaggggt tgcgccggca ggacggccag
     6661 ccgcccgagc agcccggaga ggcggcggcg gccgaggccg aggtggtgct gctcgacgac
     6721 aagtcgggcg agagctgggc cgttccgctc aagaacatca ggttggctcg actcgagatc
     6781 gagctctgaa agagatcagg aagaagaagc catgccccag gcacagaagg cacagaaggc
     6841 tcaggccgct caggacgtga atttcaacga gatcctgcgt caggtctcgc gcgagaagga
     6901 catcgatctg gaccgctggg tcgccgccct cgaggacgcc atggcctcgg cggccaagaa
     6961 gcagcaccgg atcaaggagc cggtgcgcgc ccacctcgac cgcgacaccg gccgcttcga
     7021 cgccttcatc gtcaagaagg tggtggagac ggtcgaggac ccgctggccg agtggaccgt
     7081 cgaggaggcg cgcgaccaca aggacggcgc caacgtcggg gacgagatcc acctccccat
     7141 ctccaccgac ggcctggggc ggatcgccgc ccagtcggcc aagcaggtgc tctaccagcg
     7201 cgtacgcgag gccgagcggg agaacatcta caacgagttc atcgaccgcg tcggcgaggt
     7261 gctcaacggc accgtcaagc gcttcgagcg cggcgacatc atcatcgacc tgggccgtac
     7321 cgaggccgtg gtgccccgct ccgagcaggc gcgccacgag cgctacagcc agggcgagcg
     7381 catccgcgcc gtcatcgtcg aggtgcacaa gcagcccaag gggccgcaga tcgtgctctc
     7441 gcgcaccgac ccgcgcctcc tcgtgaagct gttcgagacc gaggtgccgg agatctacga
     7501 cggcacggta gtgatcaaga acgcggtccg cgcgcccggc gagcgggcca aggtggccgt
     7561 ctacagccgc gagcgcgacg tcgacccggt cggcgcctgc gtcggcatga agggctcgcg
     7621 cgtgcagtcc atcatccgcg agctgcgcgg cgagaagatc gacatcatcg agtacagcga
     7681 cgacctggtg accttcgccc agagcgccct cgccccggcc aagatcaccc gcgtgtcggt
     7741 gacccaccac ggcgaggtgc cgcacctgga cgtcatcgtc gaggacgagc agctctcg
//
LOCUS       DASPJI010000256         6521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_646354, whole genome shotgun sequence.
ACCESSION   DASPJI010000256 DASPJI010000000
VERSION     DASPJI010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646354"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(678..1340)
                     /locus_tag="VEL74_06665"
     CDS             complement(678..1340)
                     /locus_tag="VEL74_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62244.1"
                     /translation="MEDGAFHSAGEAHCETDSGTEVVCTFAGEHFIEDVTSRLPLISR
                     VINSDSSGDYNNYLRTLPDPDRCYIAKIWVDGFFLERGTSESHVLCNPTRPQTPPNPY
                     TLTPTPTGGSASPIVLDLDGDGFSFTSSAGGVRFDLDLDGYKERTAWLAQVWIDASHD
                     GASQPEELIPLADLGITSIDLDYVEARRRDRHGNELRFKSKARAHGHTLQVVDVFLQR
                     EP"
     gene            complement(1442..2407)
                     /locus_tag="VEL74_06670"
     CDS             complement(1442..2407)
                     /locus_tag="VEL74_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62245.1"
                     /translation="MNELEQAAAEGIRVQREQRAALQAYSEAHGGELRRRDADMKVRE
                     EAACAAQAKALEAARARFGRPLFDRYLYEVVAPAMGRISQRSENRDWLRQQDTGCSAA
                     KRFAAAQEGPSAADRQPGFEAKEPWEWTLYERLERRFDPQDMDRRLAEAEARAGRPAP
                     EPGTTISIVNGRREPELLLPGEIFSRLLRLGFVLTSNPENRDTWRTGFAQGAEKLGLG
                     EHFLDELEKVTAGAIRAHREEEAAHLEASKGTHSDRKRLEEEAEQRARAQCAAQATAL
                     EAARARFGRTLFDRYLYEVVALEMSSSSQGFQSEDMLRWHETGCP"
     gene            3012..3881
                     /locus_tag="VEL74_06675"
     CDS             3012..3881
                     /locus_tag="VEL74_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HYG62246.1"
                     /translation="MTTNERATGRDVILAIGENMRSSLEPLLTKTLAPSLYQVYLHSE
                     DYDRLRSLFTELEAEAKKHLDGELERMNRQSSPSWNRLLSPLLKKKPEEPAAVPSPQY
                     VSAEGRWSVRFQEDPNGTLAPGEIEVVSEFAHQEDLEYGSGSKTHRISTTRKLGQMTS
                     RRESKTIDTQAAYARILYKDDKGPHTYLMTKDEIVIGRDAPDVWTDLRLETSLDVSRE
                     HARLQRTPQGTFRVKDLSKLGTTVEGVPVPRSLEAAEGGVRDLDRWVDLPDKARIGLA
                     GVVVLEFERLGRG"
     gene            3956..5737
                     /locus_tag="VEL74_06680"
     CDS             3956..5737
                     /locus_tag="VEL74_06680"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HYG62247.1"
                     /translation="MARAIGQQGTVSPLVPLRRLAAASASDPGRERDGNEDRVLCDPE
                     RGIFAVIDGVGGESGGEVAAQIALDTLRGRLSRRTTDAERLVREAIAQANKQIFDRAQ
                     AERGLAGMACVLTVAVLDGPQVTVGHVGDSRMYLLRKGEIRKVTRDHSPVGSREDAGE
                     LSEADAMRHPRRNEIFRDVGSGPHQPDDPEFIETYRLDLDPESALLICSDGLSDMVAS
                     RDILAIAERHAGNPSAMVDELIDAANAAGGKDNVSVVVAEGERFAASIRPVPPPAAIT
                     SIRGAASAKPEGMARWRGPLTGVLALLLILALGWFFREPLLRLYQGWRGGGTSAGGGA
                     TAPASGSVVAGDVIRVGPGEGELRSITEALAAAQPGQTVMVAPGEYTGPFEMRDGVSL
                     VSATPRGAVLRAPQVVAPAAGTGQAPVDPVVLAVNVRGARLAGFRIAGTPEAAMAVGL
                     RLVDSELEVEDLEVMGATVAAVEVSGGGQSTFRFNSIHGNPGAGVVLTGGASPRMLQN
                     LIAENGTAPRQPRPALEIGPGSRPSLIENRILRNGSGGSPQVRVAEAESVAEIYNWND
                     FGGVSRGRAIQVRPATSQTTQNTGRGR"
     gene            5734..>6521
                     /locus_tag="VEL74_06685"
     CDS             5734..>6521
                     /locus_tag="VEL74_06685"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYG62248.1"
                     /translation="MTLLLHQQKVGRFEIRDFLGRGAIGDVLLAWDPQSQAEVALKVV
                     RIGKSDPEMLEAEKNGIALQAQIARVAPQVAAVHEQGVDGDFYWVCMEYVPGNDLSRV
                     LDRGALPEERAVHIARQLCAMLEVCHGFIAEVGERRIHGIVHGDIKPENIRLQDEDRV
                     RVLDFGIAKHLSNTRRFTVNLFGSLPYTPPERLDRGVVDRHSDLWAVGVVLYIMASGR
                     RPFAGRTPEEVEQSIMAGDLQPLPETVSPALQRIIRKSLAFSVE"
BASE COUNT         1020 a         2258 c         2226 g         1017 t
ORIGIN      
        1 gtcggcttcg aggtccagcg actgctccgt cctctccatc gggggtagtg tacgggagcc
       61 tgccacggcg gccgtccccg cggaaaagac gttcataccc agggtaggag agccataccc
      121 ggagggtgga cggtctcccc gtgggtactg cggccatccc cgaagggcgg gcgaacgtac
      181 ccaggaccag gctgccatcc ccgcgggtaa gggtccatcc ccgtgaatag ggtgtccatc
      241 ccgcgaggga tgagggtcat cccgggtctc gcaaccgctg aacggcccat tcccattggc
      301 cccaggaggc ggaggacgat ccctccgcct cccatgcagg cgcgtcggcc gctgcccagg
      361 ggcgcggcct tggactcggc ggcgcccctg caagattcag ttgtcaaaga acctgcccgt
      421 catctttcgg gacgatagcc cgtagctgac gtcttgcagg agtgtaggac aatgcagtcc
      481 gccttgtcat gtccatgctt gacattaagg gcttcgttga tgacaatgcg gatgatgtga
      541 aggctgcatt gcttctgttg catggttctt tgacaatcga tggaccaggt cccgggcacg
      601 gcgagggcag cgccgtgccc ggccatgcga ggacggctcg ccgatagatc ctgaggacga
      661 tcgacgtttc aggtcggcta aggctcgcgc tggaggaaga cgtccacgac ctgaagggtg
      721 tggccgtgtg cccgggcctt gctcttgaag cgcagctcgt tgccgtgccg gtctcgccgc
      781 cgcgcctcga cgtagtcgag gtcgatcgag gtgatgccaa ggtccgccaa ggggatcagc
      841 tcctcgggtt gcgaggcgcc gtcgtggctg gcatcgatcc acacctgtgc cagccaagct
      901 gtccgttcct tgtagccgtc caggtcgagg tcgaagcgga cgccgccggc actgctggtg
      961 aaggagaagc cgtcaccgtc aagatccagg acgatcggag aggccgagcc acccgtcggc
     1021 gtcggcgtga gggtgtaagg gttgggcggg gtctggggac gggtgggatt gcacaggaca
     1081 tgggactctg acgttccgcg ctcgaggaag aagccatcga cccagatttt ggcaatgtag
     1141 cagcggtcgg ggtcgggaag agtccgtaga tagttgttgt agtcaccgga gctgtcgctg
     1201 ttgatgaccc gggaaatgag gggaagcctg cttgtaacgt cctcgatgaa gtgttcaccc
     1261 gcgaaggtac agaccacttc ggtgccggag tcagtctcgc agtgcgcctc gcccgcggaa
     1321 tggaaggccc cgtcttccat cttggtcaga ccggcatctg ccttgatcct tgttcccgcc
     1381 acggccgatg ccggaaggac tagaagggag aggacgaaca gtctacagag tcggtgcctg
     1441 gtcatgggca gcccgtctcg tgccagcgaa gcatgtcctc ggactggaag ccttgggagg
     1501 aactgctcat ctccagagcg accacctcgt aaaggtagcg gtcaaacagg gtgcgtccga
     1561 accgggccct ggcggcttcc agcgccgtcg cttgcgctgc gcattgggct cgcgcccgtt
     1621 gctcggcttc ctcctccagg cgcttgcggt ccgagtgcgt ccccttggac gcctcaagat
     1681 gggcggcctc ctcttcccga tgagcccgaa ttgccccggc ggtgactttc tcgagctcgt
     1741 ccagaaaatg ttccccaagc cccaattttt cggcaccctg ggcgaagccc gtcctccagg
     1801 tgtcccgatt ctcaggattc gaggtcagta cgaaacctag ccgcagcagc cgactgaaga
     1861 tctctccagg caacaagagc tcgggctcgc gacggccgtt cacaatggaa atggtcgtcc
     1921 cgggctccgg agcggggcgg cccgcccggg cctcagcctc cgccagtcgg cggtccatgt
     1981 cctgcgggtc gaaacggcgc tccagccgct cgtagagggt ccattcccag ggctccttcg
     2041 cttcgaaacc tggctgccgg tctgctgccg agggcccctc ctgggcggcg gcaaaccttt
     2101 tggcggccga gcagccggtg tcctgctggc gaagccagtc cctgttttcg gagcgttgtg
     2161 agatgcggcc catggcaggg gcaaccacct catagagata tcggtcgaag aggggccgtc
     2221 cgaaacgtgc cctggcggct tccagagcct tcgcttgcgc ggcacaggcg gcctcctccc
     2281 gcaccttcat gtccgcgtcc ctcctcctga gctcacctcc gtgggcctcg gagtacgctt
     2341 gcagcgcggc cctctgctct cgctgcaccc gaattccctc ggcggcggcc tgttcgagct
     2401 cgttcaagaa gccgtctccc agccctgacg ccacggcacc ctgggcgaag cctttccgcc
     2461 agctctcccg agtctcgggg tctctgacgg catagccctg ccagagcagc cacctgaaga
     2521 tctccccggg gagcaagagc tccggctccc ggctgccgtc caccaaggac agagtggtgc
     2581 cccgctcggg agagggacgg cctgcctcgg tcgctgcctc ggccaggcga cggtccatgt
     2641 cctgggggtc gaaacggcgt tccagccgtt cgtcgagggt ccactcccag ggtctctttg
     2701 cctcgggggc cgccggcgac tcccctcggc cggcggtgct ccccaaggcc gtcgccagac
     2761 aaaggaaagt caggaggaac gagcgcgacg tggaacgaag cattgcggat cttcccttta
     2821 agtttcaagc aatacttggg agcgtagcaa ccctgcaggc ggccggtcaa ggagaggtcc
     2881 ccagaaaacc gagagcctgg aacgtcttgc cccgtgcaac ctttctcctc cctgcgcgtc
     2941 tctccggtaa gatgaacggc tgtatccctc tccggtgacc gggggagggc gcctggaggc
     3001 cttctagggt gatgacgacg aatgaacgcg cgaccggcag ggacgtgatc ctcgccatcg
     3061 gtgagaacat gcggagcagc ctggagccgc tcctgaccaa gacgctggcg cccagcctct
     3121 accaggtcta cctgcacagc gaggactacg accgcctgcg ctccctcttc accgagctcg
     3181 aagccgaggc caagaagcac ctcgacggcg agctggagcg catgaaccgc cagtcctccc
     3241 cgtcctggaa ccggctgctg tcgccgctgc tcaagaagaa gccggaggag ccggcggcgg
     3301 tgccctcgcc gcagtacgtc agcgccgagg ggcggtggtc cgtccgcttc caggaggacc
     3361 ccaacggcac cctggcgccc ggcgagatcg aggtggtctc cgagttcgcc caccaggagg
     3421 acctggagta cggctcgggc tccaagaccc accgcatctc caccacccgc aagctgggcc
     3481 agatgacctc gcggcgcgaa agcaagacca tcgacaccca ggccgcgtac gcgaggatcc
     3541 tgtacaagga cgacaagggg ccgcacacct atctgatgac caaggacgag atcgtcatcg
     3601 gccgggacgc gccggacgtc tggaccgacc tgcgcctgga gacctcgctc gacgtctccc
     3661 gcgagcacgc ccggctccag cgcacgccgc aggggacgtt ccgcgtcaag gacctgagca
     3721 agctgggcac cacggtcgag ggtgtccccg tgccgcgcag cctcgaggcg gcggaggggg
     3781 gcgtgcgcga tctggaccgc tgggtggacc tgccggacaa ggcccgcatc ggcctcgccg
     3841 gcgtggtcgt cctcgagttc gaacggctcg gacggggata gcggacgatg gcctccctct
     3901 tcctcgcccg ctgggccttc ctcctggccc tggttttgct gggggtcctc ttcttatggc
     3961 tcgcgcgatc ggacaacagg ggactgtaag ccccctcgtt ccgctgcggc gcctcgccgc
     4021 ggcgtcggcg agcgacccgg gacgggagcg ggacggcaac gaggaccgcg tcctctgcga
     4081 ccccgagcgc gggatcttcg ccgtcatcga cggcgtcggc ggcgagtccg gcggcgaggt
     4141 ggcggcccag atcgccctcg acaccctgcg cgggcggctg tcgcgccgca ccaccgacgc
     4201 cgagcgcctg gtgcgcgagg ccatcgccca ggccaacaag cagatcttcg accgcgccca
     4261 ggccgagcgc ggcctcgccg gcatggcctg cgtcctcacc gtcgccgtgc tcgacggccc
     4321 ccaggtgacc gtcggacacg tgggcgacag ccgcatgtac ctgctgcgca agggggagat
     4381 ccgcaaagtc acccgcgacc actcgccggt gggctcgcgc gaggacgccg gcgagctgtc
     4441 cgaggccgac gccatgcgcc acccgcggcg caacgagatc ttccgcgacg tcggctccgg
     4501 cccccaccag ccggacgacc cggagttcat cgagacctac aggctcgacc tcgacccgga
     4561 gagcgccctg ctgatctgca gcgacggcct ctccgacatg gtggcctcgc gtgacatttt
     4621 ggccatcgcc gagcgccacg ctggcaatcc cagcgccatg gtggacgagc tgatcgacgc
     4681 cgccaacgcc gccggcggca aggacaacgt gtcggtggtg gtggcggagg gggagcgctt
     4741 cgctgccagt atccgtcccg tgccgccccc tgcggccatc acctccatcc gcggcgccgc
     4801 cagcgccaag cccgaaggga tggcccgctg gcgcggcccg ctcaccggcg tcctcgccct
     4861 cctcctcatc ctggccctgg gctggttctt ccgcgagccc ctcctccgcc tctaccaggg
     4921 gtggcgcggc ggggggactt ctgcgggagg cggcgccacg gcgccggcct ccgggagcgt
     4981 cgtggcgggg gacgtgatcc gcgtcggccc cggcgagggg gagctccggt cgatcaccga
     5041 ggcgctggcc gcggcgcagc cggggcagac cgtgatggtg gcccccggcg agtacaccgg
     5101 gccgttcgag atgcgcgacg gcgtgagcct ggtgagcgcc acgccgcgcg gcgcggtcct
     5161 ccgcgcgccg caggtggtcg ctccggcggc gggcacgggc caggcgccgg tcgatcccgt
     5221 cgtcctggcg gtgaacgtcc gcggcgcccg cctggccggc ttccggatcg ccggcacgcc
     5281 cgaggcggcc atggcggtcg gcctgcgctt ggtggactcc gagctcgagg tcgaagacct
     5341 ggaggtgatg ggggccacgg tggcggccgt cgaggtgtcg ggcggcggcc agtcgacctt
     5401 ccgcttcaac tcgatccacg gcaaccccgg cgccggcgtg gtgctcaccg gcggcgcctc
     5461 gccgcggatg ctccagaacc tgatcgccga gaacggcacg gccccgcgcc agccgcggcc
     5521 cgccctcgag atcggcccgg ggagccggcc gtccctgatc gagaaccgga tcctccgcaa
     5581 cggcagcggc ggcagccccc aggtacgggt cgccgaagcc gagtcggtcg ccgagatcta
     5641 taactggaac gacttcggcg gcgtctcgcg ggggcgggcc atccaggtgc ggcccgccac
     5701 ctcgcagacg acccagaaca cggggagggg acggtgacgc tgctcctcca tcaacagaag
     5761 gtcggccgct tcgagatccg cgacttcctg ggccgcggcg ccatcggcga cgtcctcctg
     5821 gcctgggacc cccagagcca ggccgaggtg gcgctcaagg tcgtccgcat cggcaagtcc
     5881 gaccccgaga tgctggaggc ggagaagaac ggcatcgccc tccaggcgca gatcgcccgc
     5941 gtggcgccgc aggtggcggc ggtccacgag cagggggtgg acggcgactt ctactgggtc
     6001 tgcatggagt acgtgcccgg caacgacctg tcgcgggtgc tcgaccgcgg cgcgctgccg
     6061 gaggagcggg cggtccacat cgcccgtcag ctctgcgcca tgctggaggt ctgccacggc
     6121 ttcattgccg aggtcgggga gcggcggatt cacggcatcg tgcacggcga catcaagccg
     6181 gagaacatcc ggctccagga cgaggaccgg gtgcgggtgc tggacttcgg catcgccaag
     6241 cacctgtcca acacccgccg gttcacggtc aacctgttcg gcagcctccc ctacacgcct
     6301 cccgagcggc tcgaccgcgg cgtggtggac cgccactccg acctctgggc ggtgggcgtc
     6361 gtcctctaca tcatggcgag cggccggcgg ccgttcgccg gccgcacccc cgaagaggtg
     6421 gagcagagca tcatggccgg cgacctccag ccgctgcccg agaccgtctc gccggcgctc
     6481 cagcggatca tccgcaagag cctggccttc tcggtggaga g
//
LOCUS       DASPJI010000257         3753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_477916, whole genome shotgun sequence.
ACCESSION   DASPJI010000257 DASPJI010000000
VERSION     DASPJI010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477916"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VEL74_06690"
     CDS             complement(<1..710)
                     /locus_tag="VEL74_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HYG62249.1"
                     /translation="MQLGILGLPKSGKTTLFNTLTASHQATDKYATSSQTNMGVATVR
                     DQRLEKLRALFNPKRYVPATVQYVDIPGLKRGESAESLDLAKLKTVDALVHVVRAFDD
                     PEILHSEGSVDPARDVANIDLELILADHDIVERRIDRLEKAVKRGLTPEEQKEKALLT
                     DVILPALEAETPLREVQLDPDDERRLRGFQLLSAKPMLVVINVDESRVAESPAALGIP
                     LRPAVVAVTVSAPIEQEIG"
     gene            complement(791..1678)
                     /locus_tag="VEL74_06695"
     CDS             complement(791..1678)
                     /locus_tag="VEL74_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYG62250.1"
                     /translation="MNCDDAIERLPWLLNGTLEEEERRQVQDHLAACERCRTALAETR
                     DAWRIFAGHIPTEDLVAHAWGEPTGTDPELLADHLAGCAQCTAELEMVRTSRQLEEDD
                     RVALFAPRPQSAPAVPPVAHDARGSAGKWRAAALAASLAGLVAAGGWFENAGRLRATE
                     ERLAAQEREPAAAPAGELEPLANVPIVELFAITVRGGAEDIPPESPAAHLVLSFGEAP
                     AYDEYAVEFQDASGAVRLSLQESVRSNPVTGEYTVLLPQGNLPPGEYTLVAYGLENGR
                     RTGEPVRFPLTVTGAPAAP"
     gene            complement(1668..2288)
                     /locus_tag="VEL74_06700"
     CDS             complement(1668..2288)
                     /locus_tag="VEL74_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYG62251.1"
                     /translation="MATQDQELVARFLRGEPDAVATVNDWISRAAWPYQRRLGNRRDD
                     VLQDVRLEVTRLLSQGKFRGESSLKTYLWRVVSHTCLDQIRSQGKWQWADLETLDQGE
                     EPVASVQPLPSSREDKDLLLRVLARSSQDCRELWRMVVAGFSYREMSQRLGVAEGTLR
                     VRVLRCREKALALRQELTVPLGQGYGRNKSVKSPAYEAGKVNADEL"
     gene            2391..>3753
                     /locus_tag="VEL74_06705"
     CDS             2391..>3753
                     /locus_tag="VEL74_06705"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG62252.1"
                     /translation="MTSLFISASARLLRLSALSGSLLLLAFQAGHTPAFSLERKAARG
                     ALQECRSWRAAAAGSADVDQEALEKAEAKGLEALRFSRQAHDPAGQATAHVHLGEIYG
                     WRGEVPRAVAAFEEALNLATAAGDRRGQAAALKSWGVLRAGLKEWQSALALLEEALPL
                     WKELGDGPEQGRTLYLIGVALDRLGEKGRAVEHLQEALPFYRGDPGGEADVRNALGGI
                     ELVQGRNASALAHFQRALRLSLEEEDLPVEAAVRQGLGSLYQRLGDLQSALDSYRRAL
                     SINEKVGEAVGQGRVLNSLGALYRDMGDPERALESYWQALRVLPADARADRAEVSFQM
                     GRVHYLEGGDPEKAREQFEQALSLSSEGSNTRASALFGMGLVRRSLESPAAALPLLEE
                     ALALRRQRVNRLQEASVLLEIGRTYQAMGEMEKARSFMEQALEISRRVEGPLVEAAAL
                     FRIA"
BASE COUNT          515 a         1305 c         1335 g          598 t
ORIGIN      
        1 ccgatctcct gctcgatggg ggcgctcacg gtcacggcca cgacggccgg gcggagcggg
       61 atgcccaggg cggcggggga ctcggcgact ctcgattcgt cgacgttgat caccaccagc
      121 atcggcttcg ccgagaggag ctggaagccg cggagccggc gctcgtcgtc ggggtcgagc
      181 tggacctcgc gcagcggggt ctccgcttcg agcgcgggga ggatcacgtc ggtgagcagc
      241 gccttctcct tctgctcctc cggggtcagg ccgcgcttga ccgccttctc cagccggtcg
      301 atgcgccgct ccacgatgtc gtggtcggcc aggatcagct cgaggtcgat gttggcgacg
      361 tcgcgggcgg ggtcgacgct cccctcggag tggaggatct cggggtcgtc gaaggcccgc
      421 accacgtgga ccagggcgtc cacggtcttg agcttcgcca ggtccaggct ctcggcgctc
      481 tccccgcgct tcaagccggg gatgtccacg tactgcaccg tcgccggcac gtagcgcttc
      541 ggattgaaga gggcgcggag cttctccagg cgctggtcgc gcaccgtggc cacgcccatg
      601 ttcgtctggc tcgacgtagc gtacttgtcc gtcgcctggt gcgaggcggt cagcgtattg
      661 aagagcgtcg tctttcccga cttgggcaaa ccgaggatgc cgagttgcac gggtttcccc
      721 ctccgaaggt cctgcgtggg agggggaggt taccgttttg ggttccggca ggtcaaatgg
      781 ggcaggtaaa tcagggggcg gcgggcgccc ccgtcacggt cagggggaac cgaacgggct
      841 cgccggtccg tctgccgttc tcgagaccgt aggcaacgag ggtgtactct cccggcggca
      901 ggtttccctg gggaagcaac actgtgtact caccggtcac cggattgctg cggacgctct
      961 cctggaggga caggcggacc gctccggacg cgtcctggaa ctcgacggcg tactcgtcgt
     1021 aggcgggagc ctcgccgaag ctcagcacca ggtgcgccgc cgggctctcc gggggaatgt
     1081 cctcggcccc gcccctgacg gtgatggcga acagctccac gatggggacg ttggccaacg
     1141 gctccagctc acccgccggc gcggcggcgg gctccctctc ctgcgcggcg aggcgctcct
     1201 cggtcgcgcg caggcggccg gcgttctcga accagccgcc ggcggccacc aggccggcga
     1261 ggctcgcggc cagcgcggcg gcgcgccact tgccggcgga gcccctggcg tcgtgggcta
     1321 cggggggcac ggccggcgcg gactgggggc ggggagcgaa aagggcgacg cggtcgtcct
     1381 cttcgagctg gcggctcgtc cgcaccatct cgagctcggc cgtgcactgc gcacacccgg
     1441 ccaggtggtc ggcgagcagc tcggggtcag tcccggtggg ctcgccccag gcgtgggcca
     1501 ccaggtcttc ggtggggatg tgcccggcga agatcctcca ggcgtcccgg gtctcggcca
     1561 gggcggtccg gcagcgctcg caggccgcga ggtgatcctg cacctgccgg cgttcttcct
     1621 cctcgagggt gccgttcagc agccagggga gccgttcgat cgcgtcgtca cagttcatcc
     1681 gcatttacct tcccagcctc gtaggcggga gatttcacgg atttgttacg tccatatccc
     1741 tgccccaggg ggactgtcaa ctcctggcgc agggcgaggg ccttctcccg gcagcgcagg
     1801 acccgcaccc tcagggtccc ctcggccacg ccgagccgct ggctcatctc gcggtaggag
     1861 aagcccgcca ccaccatccg ccagagctcc cggcaatcct gcgaggagcg agccaggacg
     1921 cgcagcagca ggtccttgtc ctcccgcgag gatgggagcg gctggaccga tgcgaccggc
     1981 tcctccccct ggtccagggt ctccaggtcc gcccactgcc acttgccctg gctgcggatc
     2041 tggtccaggc aggtgtggct caccacccgc cagagatagg tcttgaggct gctctcgcca
     2101 cggaacttcc cttgggagag gaggcgcgtc acctccaggc gcacgtcctg gagcacgtcg
     2161 tcccggcggt ttcccagccg gcgctggtag ggccacgcgg cgcgcgagat ccagtcgttt
     2221 acggttgcca ccgcatccgg ctcgccccga agaaaccggg cgaccaactc ctggtcctgg
     2281 gtcgccatcg ataagatcct atctctatat acatggaaag cgggccattt ctgacaccat
     2341 tccgtcttct ggagtatgat ggacgatacg tcagatctgc aggcccggct gtgacgagcc
     2401 tctttatctc cgcctccgcg cgactcctcc gtctctccgc cctatcgggc tcgctgttgc
     2461 tcctcgcctt ccaggcgggc cacacgccgg cgttctctct ggagaggaag gccgcgcgcg
     2521 gcgcgctcca ggagtgcaga tcctggcgcg cggccgccgc cgggtcggcc gacgtcgatc
     2581 aggaggcgct ggagaaggcc gaggccaagg gcctcgaggc cctgcgcttc tcgcggcagg
     2641 cccacgatcc ggccggccag gccacggccc atgtccatct cggcgagatc tatggctggc
     2701 ggggcgaggt cccgcgggcc gtcgcggctt tcgaggaggc gctgaatctc gccaccgccg
     2761 ccggagaccg ccgggggcag gcggccgccc tgaaaagctg gggcgttctt cgtgcgggcc
     2821 tcaaggagtg gcagtcggcg ctcgctctcc tggaggaggc ccttcctctc tggaaggagc
     2881 tcggggacgg accggagcag ggacggactc tttacctgat cggagtggcg ctggaccggt
     2941 tgggcgagaa gggccgggcg gtcgagcacc tccaggaggc actgccgttc taccgcggcg
     3001 atccgggcgg cgaggccgac gtccgcaacg cgctcggcgg catcgagctc gtccagggcc
     3061 gcaacgccag cgccctcgcc cacttccagc gcgccctgcg cctgagcctc gaggaggaag
     3121 acctgcccgt cgaagcggcg gtgcggcagg ggctgggctc tctctaccag cgcctgggcg
     3181 acctgcagtc cgccctcgac tcctaccgcc gcgccctctc catcaacgag aaggtcgggg
     3241 aggccgtcgg ccagggcagg gtgctcaaca gcctcggcgc cctgtaccgc gacatgggcg
     3301 accccgagcg ggcgctggaa tcctattggc aggcgttacg ggtcctgccc gcggacgccc
     3361 gggccgaccg ggcggaggtg tccttccaga tgggccgggt ccattatctg gaaggagggg
     3421 acccggagaa ggcgcgggag cagttcgagc aggcgctctc cctgagctcc gagggctcga
     3481 acacccgggc ctcggcgctc ttcggcatgg gcctcgtccg ccgctccctg gaatcccccg
     3541 ccgcggccct gccgctcctc gaggaggcgc tcgccctccg gcgccagcgc gtcaaccgcc
     3601 tgcaggaggc gtcggtcctg ctggagatcg ggcggaccta ccaggccatg ggcgagatgg
     3661 agaaggcgcg ctcctttatg gagcaggcgc tggagatcag ccggcgcgtc gaaggccccc
     3721 tggtggaggc ggccgccctg ttccgcatcg ccc
//
LOCUS       DASPJI010000258         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_760083, whole genome shotgun sequence.
ACCESSION   DASPJI010000258 DASPJI010000000
VERSION     DASPJI010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760083"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VEL74_06710"
     CDS             complement(<1..788)
                     /locus_tag="VEL74_06710"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HYG62253.1"
                     /translation="MTKSITRRVRQSNVVGGCGCWAMSLGFLLVGGLLVYFFFVRPAQ
                     RILEARSWQPVSCTIVESHVQEHSGDDGSTYSVAVAFTYSVGDAEYRSDRYKFMGGSS
                     SGYDSKARVVASLPPGTRTTCWVNPADPTDAVVNRGFTPDLLVGLIPMVFALAGLTGV
                     ISLASGSLFQKRPGTRAAAPGGTLRAHGGAPAGPVALEPKTGPLGKLAGMIFVAVFWN
                     GIVALVFQDEIAAVRAGQVPGEVAGPVIFGLLGLAMIGGVGYFFL"
     gene            complement(889..>2272)
                     /locus_tag="VEL74_06715"
     CDS             complement(889..>2272)
                     /locus_tag="VEL74_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HYG62254.1"
                     /translation="EGVGPANPDIEELNIPPLAVSDDGESLLYMKDAQWYVVPTSKPV
                     EEGQAPLDLSGMQVRIDPRAEWEQMYREVWRLQRDFFYDPGLHGLDLKATAERFRAYL
                     PDLGDRSDLNYLFHEMLGLLTVGHLGVRGGDEPQVERVPGGLLGADFEIENGRYRFRR
                     IYGGENWNPELSAPLTQPGVNVAAGEYLLAVNGRDLRSSDEVYAFFEGTAGKPVTIRV
                     GPRADGQSSREVTVVPVGSETQLRLLAWVEGNRRKVDELSQGRLAYVYLPDTGEGGYT
                     YFNRYFFAQVGRQGAILDERFNGGGMAADYIIDYLRRPLLNYWTTREGEDFTTPLGSI
                     HGPKVMMINGFAGSGGDAMPWYFRRTQIGPLVGTRTWGGLVGIYDYPELLDGGQITAP
                     RVAFFTPEREWEVENRGVAPDIEVELDPQAWRQGRDPQLEKAVEVALDLLAKNPPPKK
                     PQRPAYPDYN"
BASE COUNT          349 a          788 c          797 g          338 t
ORIGIN      
        1 aggaagaaat atccgacgcc gccaatcatc gccaagccca ggaggccgaa gatgacgggg
       61 ccggccacct cgcccggcac ctgccccgct cgcaccgccg cgatctcgtc ctggaagacc
      121 agggcgacga tgccgttcca gaagacggcg acgaagatca tgccggcgag cttgcccagc
      181 ggtcccgtct tgggctccag ggccaccggg ccggccggcg cgccgccgtg agcccgcagg
      241 gtgccgcccg gggccgcggc cctggtgccg gggcgctttt ggaagaggct gccggaggcc
      301 agggagatca ccccggtgag gccggcgagg gcgaagacca tggggatgag cccgaccagc
      361 aggtcagggg tgaagccccg gttgaccacg gcgtccgtgg ggtcggcggg gttgacccag
      421 caggtggtgc gcgtgccggg cggcagggag gccacgaccc tcgccttgga gtcgtagccg
      481 gagctggagc ctcccatgaa cttgtagcgg tcggaccggt actcggcgtc ccctaccgaa
      541 taggtaaagg caaccgcgac actgtaggtg ctgccgtcgt ccccggagtg ctcctggacg
      601 tggctctcca cgatggtgca ggagaccggc tgccaggagc gggcttcgag gatccgctgc
      661 gccgggcgca cgaagaagaa gtagaccagc aggcccccca cgaggaggaa gcccagagac
      721 atggcccagc atccgcagcc gccgacgacg ttggactgac ggacccgccg cgtgatggat
      781 ttggtcactc gctcggttcc tgcccggatc taaccgctcc aggggggcag atgtcaagcg
      841 ggtggggaga gggagccggg gaggctcacc tcccccggcc gtctctctct agttgtagtc
      901 cgggtacgcc ggccgctgcg gcttcttcgg cggcggattt ttcgcgagga ggtccagggc
      961 cacctcgacg gccttctcga gctgcgggtc gcggccctgc cgccaggcct gcgggtcgag
     1021 ctccacctcg atgtcgggcg ccacgccgcg gttctccacc tcccactccc tctccggcgt
     1081 gaagaacgcg acccgcggcg ccgtgatctg gccgccgtcg agcagctccg gatagtcgta
     1141 gatgccgacc agaccgcccc aggtgcgggt gccgaccagc ggcccgatct gggtgcgccg
     1201 gaaataccag ggcatcgcgt ccccgccgga gccggcgaag ccgttgatca tcatcacctt
     1261 cgggccgtgg atggagccca gcggcgtggt gaagtcctcc ccctcccggg tcgtccagta
     1321 gttgagcagc ggccggcgca ggtagtcgat gatgtaatcg gccgccatgc ccccgccgtt
     1381 gaagcgctcg tcgagaatcg ccccctgccg gcccacctgg gcgaagaagt agcggttgaa
     1441 gtaggtgtag ccaccctctc ccgtgtcggg cagatagacg taggcgaggc gcccctggct
     1501 cagctcgtcc accttgcggc ggttgccctc cacccaggcg aggagacgga gctgcgtctc
     1561 gctccccacc ggcaccaccg tcacctcacg ggagctctgg ccgtccgccc gcggccctac
     1621 gcggatcgtc accggcttgc cggccgtccc ctcgaagaag gcgtacacct cgtcggaaga
     1681 gcggaggtcc cggccgttga ccgccagcag gtactccccg gcggcgacgt tgacccccgg
     1741 ctgggtgagc ggcgccgaca gctcggggtt ccagttctcc cctccgtaga tgcgccggaa
     1801 gcggtagcgg ccgttctcga tctcgaagtc ggcgccgagc aggccgcccg gcaccctctc
     1861 cacctggggc tcatcgccgc cacggacccc gaggtgcccg acggtgagga ggcccagcat
     1921 ctcgtggaag aggtagttga ggtcgctgcg gtccccgagg tccggcagat aggcccggaa
     1981 gcgctcggcc gtcgccttga ggtccaggcc gtggaggccc gggtcgtaga agaagtcgcg
     2041 ctgaaggcgc cagacctcgc ggtacatctg ctcccactcg gcgcggggat cgatccgtac
     2101 ctgcatcccc gagagatcca ggggggcctg cccctcctcg acgggcttgg aggtggggac
     2161 cacgtaccac tgggcgtcct tcatatagag gaggctctcg ccgtcgtcgg agacggcgag
     2221 tggggggatg ttcagctctt cgatgtccgg gttcgccggt cccaccccct cc
//
LOCUS       DASPJI010000259         4583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_131166, whole genome shotgun sequence.
ACCESSION   DASPJI010000259 DASPJI010000000
VERSION     DASPJI010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131166"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3582
                     /locus_tag="VEL74_06720"
     CDS             <1..3582
                     /locus_tag="VEL74_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62255.1"
                     /translation="CRAAYLSGELPVLGRNSQEGDEPADAALAPALDLADETPRAAIE
                     RALVEAVARALRLPSECLESRRPLAGLGLDSLAAVEVCHEFERVLGVPLSITEVLDSP
                     GIAHLADTISERSGHEPRPFPSSGEEDPGELPLSRGQQALLFVDRLAPESAPYNLAGA
                     AWIARGLAAAALERALGCLADRHPALRTRFELGGAEPRQRVEPRLPIDFQIEPLPDGG
                     LPARKILAAEAYRPFDLERGPLFRARVWTATGGGHLLLIALHHVIADFWSIAILLREL
                     GELYREESAGTPAHLAAPGARLAAWLRRQEQRLGGAEGERLASFWERQLAGPPAPLDL
                     PTDRPRPPVQSFLGAARSLQLETGLARGLRAFGRSCQATLFMTLLAGFQALLHRCSAQ
                     EQIVVGSPTAGRNDTGIAGTVGYFVNPVALTASFTGNPTFGGLLEQVRRTSLAAFEHQ
                     QFPLPLLLERLRTERDASRSALFQAMFVLQGSRQPGSEGLAAFLLGDPAARLELADLV
                     LEPVALEEHRSPFDLTLFAAEHGDEIVTVLQYNRDLFDGVTMARLLNHFAILAESALA
                     DPAAPVRELQLLSAAERQQVVVEWGEVGDLPADGALVHQQFAAQAAAQPDRVAVLCGE
                     EQTTYGELRRRAARLAHRLRALGVESEERVAICLDRSPEMVASILGVLEAGGAYLPLD
                     PAYPEERLAFSLEDSRARVLLTRGDVRLATRPGLQVVDLDRLERDAAPRATGPAPPVS
                     RESLAYVIYTSGSTGRPKGVMIEHRSAAALVDWAQRTFVPAELAGVLAATSICFDLSV
                     FELFAPLSTGGTVILAKDALSLVDLPAAGRVTLVNSVPSVMAELVYRGGISPSVRTVC
                     LAGEPLSRALTERVYAASNGMNQVWNLYGPTEDTTYSTSSAVPRSGQGEPTIGRAVAG
                     SRASVLGLDLQPVPMGVVGELCLAGAGLARGYLGRPDLTAERFVPDPFAESPGERLYR
                     TGDLVRHRTNGEIEFLGRRDTQVKVRGFRIELGEIEATLCIHPDLREAVVLPRGAGAD
                     VSLVAYVVPRTGASPETEELRSFLGAKLPAYMVPSVFVPLAELPVTRNGKLDRQALPD
                     PVGSPAASPYVAPRSAFEATLASLWKDVLGAERIGVHDNFFVLGGHSLKASQVLSKIR
                     SGFGVDLPMRSLFEAPTIAGLAGAIVKEYARRAGQETASSLISEVEK"
     gene            3582..>4583
                     /locus_tag="VEL74_06725"
     CDS             3582..>4583
                     /locus_tag="VEL74_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG62256.1"
                     /translation="MIDPRPLASDDRIDQLSPEQLDLLLRRFQGQVVEEPDGIRPRQR
                     SSEGAPLSFAQERLWFLDQLRPGSSAYNVPVAFRLCGPLDQDALAASLDGLVRRHEIL
                     RTTFVAAEGNPIQFVSLAAECTLPFTDLSTLAPAERDRELARFRAEEASRPFDLALGP
                     LLRAALLRLAAQEHVLLLTLHHIVADATSLGLILREITALYQGTEPPELPVQYADYAV
                     WQREWLQGERLAAQLAYWRHQLAGAPMTLELPADRPRPPVQSFRGASRRLGLPDGAAA
                     RLRQTALREGVTPYMLLLAGFAALLARCTGQEDLLIGTPVDIRTRPEVERLIGFFVNT
                     "
BASE COUNT          596 a         1735 c         1547 g          705 t
ORIGIN      
        1 tgccgcgccg cctacctgtc cggagagctc cccgtcctcg gacgcaactc gcaggaaggg
       61 gacgagccag cagacgccgc gctcgctccg gccctggatc tcgcggacga gacaccccgt
      121 gccgccatcg agcgcgcgct ggtcgaggcc gtcgcccgcg ccctgcggct cccctccgag
      181 tgccttgagt cgcggcggcc tctcgccggg ctgggcctcg actcgctggc cgcggtggag
      241 gtgtgccacg agtttgaacg cgtcctcggc gtgccgctgt ccatcacgga ggtcctcgat
      301 agccccggca tcgcgcacct cgcggacacg atcagcgaac gatcgggcca cgagccgcgg
      361 ccgttcccct catccggaga ggaggatccc ggcgagctcc ccctctcccg gggacaacag
      421 gccctcctct tcgtcgaccg tctcgcaccg gagagcgcgc cctacaacct ggccggcgcg
      481 gcctggatcg cccgcgggct cgctgccgcg gcactcgagc gcgccctggg ctgtctggcc
      541 gaccggcatc cggccctgcg cacccgcttc gagctggggg gagccgagcc gcggcagcgt
      601 gtggagcccc gcctgcccat cgatttccag atcgagcccc tcccggacgg cggtctcccc
      661 gcgaggaaga tcctggcggc cgaagcctac cgcccgttcg acctcgagcg cgggccgctc
      721 ttccgggcac gggtgtggac cgccaccggg ggtggccacc tcctcctgat cgcgctgcac
      781 cacgtgatcg ccgatttctg gtcgattgcc atcctgctgc gggagctggg tgagctctac
      841 cgcgaggaga gcgccggcac accggcccac ctcgccgctc ccggcgccag gctggcggcc
      901 tggctccggc ggcaggagca gcggcttggc ggcgcggagg gggagcggct ggcgagcttc
      961 tgggagcgcc agctcgccgg cccaccggca cccctggacc tgcctaccga ccggccccgc
     1021 ccaccggtcc agtccttcct cggcgccgcc cggagcctcc agctcgaaac gggcctggcc
     1081 cgaggcctcc gcgccttcgg gcgctcctgc caggccactc tcttcatgac gctcctcgcc
     1141 ggcttccagg cgctgctaca ccgctgctcg gcccaggagc agatcgtcgt ggggtctccc
     1201 acggccggaa ggaacgatac cggtatcgct gggacggtgg gctacttcgt caaccctgtg
     1261 gccctcaccg cgtccttcac cggcaatccc acgttcggcg ggctcctgga gcaggtgcgc
     1321 cggacctccc tggcggcctt cgaacatcaa cagttcccac tgcccctgct gctcgaacgc
     1381 ctgcgcaccg agcgggacgc cagccgctcc gcgttgttcc aggcaatgtt cgtcctgcag
     1441 ggctcccgcc agccggggag cgaggggttg gcggccttcc tgctggggga cccggcggcg
     1501 aggctcgagc tcgcagacct cgtgctcgag ccggtggcgc tcgaggagca ccggtcgccg
     1561 ttcgacctca ccctcttcgc cgccgagcac ggcgacgaga tcgtcaccgt gctgcagtac
     1621 aaccgggatc tcttcgacgg tgtcaccatg gcccggctgc tgaaccactt cgcgatcctc
     1681 gccgaaagtg ccctggccga tcccgcggca cccgtgcgcg agctccagct cctcagcgcc
     1741 gccgagcgcc agcaggtggt cgtcgagtgg ggagaggtgg gcgacctgcc ggcggacggc
     1801 gccctcgttc atcaacagtt tgccgcgcag gccgcagccc agccggaccg cgtcgcggtg
     1861 ctctgcggcg aggagcagac gacatatggc gagctccgcc ggcgcgccgc ccggctggcc
     1921 caccggctgc gagcgctcgg cgtggagtcc gaggagcggg tcgccatctg tctcgaccgc
     1981 tcgccggaga tggtggcgtc aatcctcggg gtgctcgagg caggaggggc ctacctgccg
     2041 ctcgatcccg cctatcccga ggagcgtctg gccttcagcc tggaggactc gcgcgcgcgg
     2101 gtgctgctga cccgcggcga cgttcggctc gccacccggc ccggcctcca ggtcgtcgac
     2161 ctcgatcgtc tggagcgtga cgcggcccca cgggcaaccg gaccggcgcc gccggtctcc
     2221 cgggagagcc tcgcctacgt catctacacc tcgggctcca ccggccgccc caagggggtg
     2281 atgatcgagc accgctcggc ggcggcgctg gtggattggg cgcagcggac gttcgtgccg
     2341 gcggagctcg cgggtgtgct ggcggcgacc tcgatctgct tcgatctctc ggtcttcgag
     2401 ctgttcgccc ccttgagcac aggcggcacg gtgatcctcg ccaaagatgc tctgtctctc
     2461 gtcgatctcc ccgcggccgg gcgggtgacg ttggtcaact ccgtcccgtc ggtcatggcc
     2521 gagctggtct accgcggcgg gatctccccc tccgtgcgga cggtctgcct ggccggcgag
     2581 cccctgtccc gggcgctcac cgagcgggtc tacgccgcct cgaacgggat gaatcaggtc
     2641 tggaacctct acggtccgac ggaggacaca acctactcga cctcctccgc ggtgccccgc
     2701 tcagggcagg gagagccgac cattggacgg gccgtcgccg gctcgcgcgc ctcggtgctc
     2761 ggcctcgacc tccagcctgt gccgatgggc gtggtgggcg agctctgcct cgccggcgcg
     2821 ggcctcgccc gcggctatct cggcaggccg gacctgacgg ccgagcgctt cgtgccggat
     2881 ccgttcgcgg agagccctgg agagcgcctc taccgcaccg gcgacctcgt gcgccaccgg
     2941 accaacggcg agatcgagtt cctcgggcgg cgggacaccc aggtcaaggt gcgggggttc
     3001 cgcatcgagc tgggagagat cgaggcgacc ctctgcatcc acccagacct ccgcgaagcg
     3061 gtcgtcctac cacgcggcgc gggggccgac gtgagcctgg tggcctacgt ggtgccccgg
     3121 accggggcgt ctccggagac cgaggagctg cgcagcttcc tcggcgctaa gctgccagcc
     3181 tacatggtgc cctcggtgtt cgtccccctg gcggagctgc cggtgacccg caatggcaag
     3241 ctcgaccgcc aggctctccc cgacccggtg ggaagtccgg ccgccagccc gtacgtagcc
     3301 ccgcgctcgg ccttcgaggc cactctcgcg agcctctgga aagacgtgct gggcgccgag
     3361 cgcatcgggg tccacgacaa tttcttcgtc ctcgggggtc attcgctcaa ggcctcccag
     3421 gtgctctcca agatccgcag cgggttcggg gtcgatctgc ccatgcgcag cctcttcgaa
     3481 gcccccacca tcgccggatt ggcaggcgcc atcgtcaagg agtacgcgcg gcgggcgggt
     3541 caggagaccg cctcgtcgct gatttccgaa gtggagaaat gatgatcgat ccgcgtcctc
     3601 tcgcgtccga tgaccgcatc gaccagctct cacccgagca gttggacctt ctcctgcgcc
     3661 gtttccaggg acaggtcgtg gaagaaccgg acggcatccg cccacggcag cgcagctccg
     3721 agggggctcc gctctcgttt gcccaggagc ggctctggtt cctggaccag ctccggcccg
     3781 ggagctccgc ctacaacgtg ccggtcgcct tccgcctgtg cggtcccctc gatcaggacg
     3841 cgctcgcggc tagcctggac gggctcgtgc gacggcacga gatcctgcgc accaccttcg
     3901 tggcggcgga ggggaacccg atccagtttg tctccctggc ggcagaatgc accctgccgt
     3961 tcaccgacct ctcgaccctt gccccagccg agcgggaccg ggagttggcc cgcttccggg
     4021 ccgaggaagc cagccggccc ttcgatctcg ccctgggtcc gctcctgcgg gcggcccttc
     4081 tgcgcctggc agcgcaggag cacgtcctgc tgctgaccct ccaccacatc gtcgccgacg
     4141 ccacctcgct cggcctcatc ctgcgcgaga tcaccgcgct ctatcagggc acggagccgc
     4201 cggagctgcc ggtccagtac gcggactacg ccgtctggca acgcgagtgg ctgcaggggg
     4261 agcggctcgc cgcccaactc gcctactggc gccatcaact cgccggcgca cccatgacgc
     4321 tcgagctgcc agccgaccgg ccgcgaccac cggtgcagtc cttccgcggc gccagccgcc
     4381 gtctgggtct cccggacggc gcggcggcgc ggctgcgcca gacggcgctg cgcgagggag
     4441 tgacgccgta catgctgctg ctcgccggct tcgcggcgct cctggcgcgc tgcaccggcc
     4501 aggaggacct gctgatcggc acgccggtgg atatccggac acgccccgag gtcgaaaggc
     4561 tgatcggctt cttcgtcaac acc
//
LOCUS       DASPJI010000260         7350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_701975, whole genome shotgun sequence.
ACCESSION   DASPJI010000260 DASPJI010000000
VERSION     DASPJI010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701975"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1655)
                     /locus_tag="VEL74_06730"
     CDS             complement(<1..1655)
                     /locus_tag="VEL74_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62257.1"
                     /translation="MAVLSAFSLPAFIQDLPAGSPEDIALKEQWNINVEGWIQQAMPT
                     LPSWFYDPTRTDIPSGTAALRVEWGAFPGRLDQYFSKQPPNPPANPYNLSDEQVYELA
                     DTGFYTDNQQSSSFPQIPATLCPQADWNGELETFGPYGPRGWLDEYCEWSAARDASGN
                     LVRVDFACENPEYWYTLWKIDPAKVCALYESVLNWDAPESRQVKVLPEDLWLYDTNGN
                     VVNDPETNLPAYNPLNKWNNSPVSQRNGDSSAFTGGVMHLTSTPNTLQTELGLAGSAT
                     LQYNSGNSDPQILICCGNYGQEYRHSDPHIGQSVNQVVGGQALGKGCLVCLADPVGLY
                     IQNLTNPAALGFGSSVNQSLLPSGAQATDIWQVVRGSASLVDPVTGQDFPGAFNLHVV
                     CQIPSAWLAGQPDLTLADITINGEPILYAGQIANQFDVGLYVRPLPAGSPAPQVPCAS
                     TAPMPGQPLQCMYSVLWNAYYAGVETAPTGATMSLASNTTMIAPQVQAGGNPLLTLTC
                     NPPSGNEAPTVSVMQANGAGPDPTIQVEVLSISLANYAVPGNS"
     gene            2042..3394
                     /locus_tag="VEL74_06735"
     CDS             2042..3394
                     /locus_tag="VEL74_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HYG62258.1"
                     /translation="MGRRSAEPVMTDKRLRSLVCPEGQKFHEFRDPERRNFFVRVYAG
                     SGLKVFFYRYRSPYPKGVPARQRPMRSVKLGSYPEEIGLAGVKSAVARIDGQVASGID
                     PQGPPPTGQKGGRPGRTAAPVTADNSRAEQLAAILGPGPVLPGSFADLAQRYLLQHVW
                     PTFRRARDIEMALAQMCAAWRDEVASTIRKGHAKELINRWKLRNKMTAARRMRTYIGM
                     VFNWGIGEDLFDDGNRLEVNPASGIRDLGRESRRTDYLQDEQIAAFWRICEARLKGHK
                     VTSSALQLLLVLIQRPGEVYQMKWEEIRGEWWYIPGGKAGRTKNELEHRVYLPPLALE
                     ILGRLGSAADRRGYVFPSPRLSDGSPVTVTGMSSVYNDLCRAAGIVCEPKPWGPHGLR
                     RTGSTKLSELGFSDEVVDAVTNHVKQGVRRNYNLNAYLPEKQKAMLAWEAHLRTIIAG
                     "
     gene            complement(3381..3719)
                     /locus_tag="VEL74_06740"
     CDS             complement(3381..3719)
                     /locus_tag="VEL74_06740"
                     /inference="COORDINATES: protein motif:HMM:NF017717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HYG62259.1"
                     /translation="MAHEQVGTLLDHLLASYREEINAIRGELAELRQEIRRDRQQPPA
                     PAHRATIRIKEVCSRSGLSRATIYRLESEGKWPRRYRLTDGTVGWDEAEVEARIAEMQ
                     ATRVGGTTPR"
     gene            complement(3728..4555)
                     /locus_tag="VEL74_06745"
     CDS             complement(3728..4555)
                     /locus_tag="VEL74_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HYG62260.1"
                     /translation="MTTPRGFSVALYARTSAADLGRETVEQLLAGLSTYASGRGWVVA
                     LECADQSPFSGGRREGLQRLAAAVRANAVQGLVVRSLSHLARSLRHLTDLGHLLVAHG
                     IALVAVEDGLDTTDPGGAIRWRDWLEISTRLDRQLRAEAAKLARLRTPGDRWGRPAAA
                     FNPLELLTWWEGRGGRRPLSLRELAGKLGVSEATARKHLQALRAAGQVNDAARLQALA
                     ARGGLRKGGRPANPLDDDTLTAAWRQTPSLAAVARTLRVSRNRVRSRLRELGFLEEK"
     gene            complement(4552..4938)
                     /locus_tag="VEL74_06750"
     CDS             complement(4552..4938)
                     /locus_tag="VEL74_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62261.1"
                     /translation="MPIRPEGARAVLARVDPTRLRSGWPVGLRDGALLALVAAGLSAE
                     EICALHASSISMDRGNLLIAVRRHGILWKAVLPTDLGARLLAWLTERRLWADPAPVFT
                     GPKGPLSRVAIHQILYRYRRQKGARG"
     gene            complement(4938..6284)
                     /locus_tag="VEL74_06755"
     CDS             complement(4938..6284)
                     /locus_tag="VEL74_06755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HYG62262.1"
                     /translation="MARHKALPHSEEAERAVLGALLLEPLRIAQVRRRLDARDWYLAR
                     HRVLYQAYLDVTDAGSTPDLLTLRAHLEQAGILGAAGGLAYLTALDLDLPDLGRLDEY
                     VEILKERSVRRDLIGDAQRTLLAATATARPVHELLADLRGSADKLLGRAARVHWEGAG
                     DVVNRLLANLEEGGSEVLCGLTSGFPGWDRLGPGFLPGGLYVIAGRPGMGKTSLALDL
                     TRHVAIELQRPVGIFSLEMHSDELGLKLGSAEAGVASRHLRAGHLSRRQWRDLLAATG
                     RMMAAPLFLDYSPGLALRDLEARAWWLKDHHPDLALLVVDYLQLLNAGVRVEHRRLEI
                     ALITRRLKELAGELGLPMIALSQLNRELTRRSDPRPQLADLAESDAIGQHADQVAFVH
                     RPEVYAPEDESLRGLAEIIVAKHRHGETGTVELAWNGATTSFRNRPATAPAGPEPA"
     gene            complement(6284..6949)
                     /locus_tag="VEL74_06760"
     CDS             complement(6284..6949)
                     /locus_tag="VEL74_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62263.1"
                     /translation="MTSWHCGRCGEDQIAFGPVPADLLCHACHEAQLGAFSEAAAGIP
                     ERYRRLTRESWTAHFRRPWPAALERWTGLPHWVALWGPTGTGKTGLATVVLAEHLRTG
                     RRGRWISGPELARRIQSDFAAAEDVLAPLLVTPLLVLDEPLSGAAADWYLERLVLLTR
                     TRDERGLPTLVTSQLLPELLLEPSTAAPPVLLSRWLSGLRLHHELAGEDVRLQETPGG
                     LAR"
     gene            complement(6946..>7350)
                     /locus_tag="VEL74_06765"
     CDS             complement(6946..>7350)
                     /locus_tag="VEL74_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62264.1"
                     /translation="PYPPIPLNPPEKQETFGLRPLAGPQSAVLRVNLLLEEAGVPLPS
                     PAQIGLWSKTLGGIEPLLELLGRLVQSGLANKRAPIVYVHRVVMERAARPEPVRPAKS
                     VNPLRTAGVDEIRRRQAARIAGRSSSGEEAGE"
BASE COUNT         1189 a         2238 c         2721 g         1202 t
ORIGIN      
        1 tacgagttgc cgggcaccgc gtagtttgcc agcgatatgg agagaacctc gacctggatc
       61 gtggggtccg ggccggcacc gttggcctgc atgacggaca ctgttggcgc ctcattgccg
      121 ctgggcgggt tgcaggtcag cgtcagcaag gggttaccgc ctgcctgcac ctgcggagcg
      181 atcatcgttg tgttgctggc caatgacatg gttgcaccgg ttggagcggt ctcgacgccg
      241 gcgtagtaag cgttccatag caccgaatac atgcactgca gcggctgccc gggcatcgga
      301 gccgtgctcg cgcacggcac ctgcggcgct gggctaccgg ccggcagcgg gcgcacgtag
      361 aggccgacat cgaactggtt ggcgatctgg ccggcataaa ggatcggctc gccgttgatg
      421 gtgatgtctg ccaatgtcag atcgggctgg ccggcaagcc aggccgacgg gatctggcag
      481 accacatgca gattgaaggc gccggggaag tcttggccgg tcactggatc cacaagagac
      541 gccgagccgc gcaccacctg ccaaatgtcg gtcgcctgag cgccgctggg cagaaggctc
      601 tgattgaccg aggagccgaa gccaagggcg gctgggttgg ttagattctg gatatagagt
      661 ccgaccggat ctgccaggca aactaggcag cctttgccca atgcctggcc gccaacaacc
      721 tgattgacgc tctggccaat gtgtggatcg ctgtggcgat attcctggcc gtagttgccg
      781 cagcagatca ggatctgggg atcggaattt ccactattgt actgcagggt cgccgagcca
      841 gccaggccca actcggtctg gagggtgtta ggcgtgctgg tcaggtgcat gaccccgccg
      901 gtgaaggccg aagaatcgcc gtttcgctgg gagaccgggc tgttgttcca cttattcaga
      961 gggttgtagg cggggagatt ggtctcaggg tcgttgacga cattgccgtt ggtgtcatag
     1021 agccacaagt cctccggcaa cactttgacc tggcgcgatt ccggcgcatc ccagttgagc
     1081 acggactcgt acagggcgca gaccttggcg ggatcgatct tccagagggt gtaccagtat
     1141 tcggggttct cgcaggcgaa atcgacacgg acaagattgc cgctcgcatc acgcgccgcg
     1201 ctccattcgc agtattcgtc cagccagccg cgcgggccgt atggaccaaa ggtttcgagc
     1261 tcgccgttcc agtctgcttg ggggcaaagg gtcgcgggga tttgcgggaa ggaggagctc
     1321 tgctggttgt cggtatagaa accggtgtcg gccagctcat aaacctgctc atcgctaagg
     1381 ttgtacgggt tggccggggg gttgggcggc tgcttcgaga aatactggtc gagccgtccc
     1441 gggaaggccc cccattccac ccgcagggcg gcggttccgg aggggatgtc ggtcctagtg
     1501 gggtcgtaga accacgacgg aagagttggc atcgcctgct ggatccatcc ttcgacgttg
     1561 atgttccatt gctcctttag agcgatgtcc tcgggtgaac ctgccggaag atcctggata
     1621 aacgcgggca aggaaaacgc ggacagaaca gccatgcttt ggctcctcct tatcaatcta
     1681 ggtgatgagt aagactattt ctatattgaa ccataaaaga ctggccggcg caaggggcgc
     1741 aaaggcgaag tcctgtctgc ggtcgtcaga tgcggagcga ttcatccgcc acctcggctg
     1801 taagtgctta gaagtgctgg gggttaggtt ggagccgacg gccgggattg aaccgacgac
     1861 ctgctgatta cgaatcagtc ctaagccttt tcaggctgtc tcagctaacg gcagcctcgg
     1921 ctcgatacgg ctgtaaacct aagagaaata aagatcttct gtctgtcgac tgtctcaggc
     1981 cgtctcatgt tatactttcc agcgtagtac ctaccgtagt acctacgatg gaggagggga
     2041 gatgggtcga cggtcggctg agccggtgat gaccgacaag cggctccggt cgctggtatg
     2101 tccggaaggg cagaagttcc atgaattcag ggacccagag cggcggaatt tcttcgttcg
     2161 tgtgtacgcg ggtagcgggc tcaaggtgtt cttctacagg taccggagcc cctaccccaa
     2221 gggggtgcca gcgcgccagc ggccgatgcg ctccgtcaag ttgggaagct acccggagga
     2281 gatcggccta gccggcgtga agagcgccgt agcgcggatt gatggtcagg ttgcaagcgg
     2341 gatcgatccg caggggccgc cgccgactgg ccagaaggga ggccggcctg gcaggacggc
     2401 ggcaccggtg acggccgaca actcgcgggc ggagcagctc gccgccatcc tgggccccgg
     2461 gcccgtgctc cctggctcgt tcgccgatct cgcccagcgc taccttcttc agcacgtatg
     2521 gccgacgttc cggcgagccc gggacatcga aatggccctc gctcagatgt gcgcggcctg
     2581 gcgggatgag gtggccagca ccatccgcaa gggccatgct aaggagctga tcaaccgctg
     2641 gaagctgcgg aacaagatga ccgcggcccg acggatgcgg acctacatcg ggatggtctt
     2701 caattggggg atcggcgagg acctcttcga cgatgggaac cgcttggagg tgaatcccgc
     2761 ctcagggatt cgggacctcg ggcgtgagag ccgacggacg gactacttgc aggatgagca
     2821 gatcgccgcc ttctggcgga tctgcgaggc tcgactcaag gggcacaagg tcacctcctc
     2881 ggcgctccag ctcctgctcg tgctgatcca gaggcccggc gaggtctatc agatgaaatg
     2941 ggaggagatc cgcggggagt ggtggtacat ccccggcggt aaggcgggac ggacgaagaa
     3001 cgagctggag caccgggtct acttgcctcc tcttgccctg gagatcctcg ggcgcctcgg
     3061 ctccgcggcg gaccggcgcg gctacgtgtt cccgagccct cggctgtctg acggctcgcc
     3121 cgtcaccgtg accggcatga gctcggtcta caacgacctc tgccgcgctg cggggatcgt
     3181 ctgcgagcca aagccctggg gtccgcacgg gctccggcgc accgggagca ccaagctgtc
     3241 agagctgggc ttctcggatg aggtcgtgga tgcggtaacc aaccacgtca agcagggcgt
     3301 acggcgcaac tacaacctca acgcctacct gccggaaaag cagaaggcga tgctcgcctg
     3361 ggaagcccat ctgcgcacga tcatcgcggg gtagtacctc ccacccgggt cgcctgcatc
     3421 tccgcgatcc gagcctcgac ctcggcctcg tcccaaccga ctgtcccatc ggtcagtcgg
     3481 tagcgacgag gccatttccc ttcgctctcg agccggtaga tagtcgcccg gctcaggccg
     3541 gagcggctgc agacctcctt gatccggatg gttgctcgat gcgccggcgc cggcggctgc
     3601 tggcgatcac ggcggatctc ctggcgaagc tcggccagct ctccgcggat ggcgttgatc
     3661 tcctccctgt acgaggccag gaggtggtcc agcaaggttc ccacttgctc gtgtgccatc
     3721 ccttctccta cttctcttcg aggaatccca gctcccggag ccgtgatcgc acgcggttcc
     3781 ggctcacccg cagggtgcgg gcgacggcgg cgaggctcgg tgtctggcgc caggcggcgg
     3841 tgagggtgtc gtcgtcgagg gggttcgcgg gccggccgcc tttccggagg cctccccggg
     3901 cggcgagcgc ctggaggcgg gcggcgtcgt tgacctggcc ggcggctcgg agggcctgga
     3961 ggtgcttgcg ggcggtggct tccgagacgc cgagtttgcc ggcgagctct cgcagggaga
     4021 gcggccggcg gcctcctcgg ccttcccacc aggtcagcag ctccagaggg ttgaaggccg
     4081 ctgcgggacg gccccagcgg tctcccggcg tgcgcaggcg ggcgagcttc gcggcctcgg
     4141 cgcggagctg gcggtccaga cgggtggaga tctcgagcca gtctcgccag cggatggctc
     4201 cgcccggatc ggtggtgtcg aggccgtctt caacggcgac cagggcgatg ccgtgggcga
     4261 cgaggaggtg gcccaggtcg gtcaggtggc ggagggagcg cgctagatgg ctcagggagc
     4321 gcacgacgag gccttggacg gcgttagccc gcacggctgc ggcgaggcgc tggagaccct
     4381 ccctccggcc tccggagaag ggactctggt cggcgcactc cagggcgaca acccagcccc
     4441 tgccggatgc gtaggtgctc aggccggcga ggagctgctc gacggtctct cggcccaggt
     4501 cggcggccga ggtgcgggcg tagagggcga ccgagaagcc cctgggcgtg gtcatccgcg
     4561 ggctcccttc tggcgccggt atcggtagag gatctggtgg atggctaccc gggaaagcgg
     4621 gcccttaggt ccggtgaaga ccggcgcggg gtcggcccag aggcggcgct cggtgagcca
     4681 ggcgagcagc cgggcaccaa ggtcggtggg cagcacggct ttccagagga tgccgtgacg
     4741 gcggacggcg atcaggaggt ttccccgatc catcgagatc gaggaggcgt gcagggcgca
     4801 gatctcttcg gcgctcaggc cggcggcgac cagcgccagg agtgcgccgt cgcgcaggcc
     4861 tacgggccag ccggagcgca gccgggtcgg atcgacccgg gcgagaaccg ctcgcgcgcc
     4921 ttctggacgg atcggcatca ggcgggctcc ggtccggccg gcgccgtggc ggggcggttg
     4981 cggaaggagg tggtggcgcc gttccaggcc agctcgacgg tgccggtctc gccgtggcgg
     5041 tgcttggcga cgatgatctc cgcgagcccg cgcaggctct cgtcctcggg ggcgtacacc
     5101 tcaggacggt ggacgaaggc gacctggtcg gcgtgttgcc cgatcgcgtc ggactcggct
     5161 aggtcggcga gctgcgggcg tggatcgctc cggcgggtca gctcgcggtt gagctgggag
     5221 agggcgatca tcgggaggcc cagctcgccg gccagctctt tgaggcggcg ggtgatgagg
     5281 gcgatctcca ggcgccggtg ctcgacgcgc acgccggcgt tgaggagctg gaggtagtcg
     5341 acgaccagga gggcgagatc gggatggtgg tccttgagcc accaagccct ggcttcgagg
     5401 tcgcgcaacg cgaggcccgg gctgtagtcc aggaagagcg gcgccgccat catgcggccc
     5461 gtggcggcga ggaggtctcg ccactgccgc cgcgagaggt ggccggcgcg aaggtggcgc
     5521 gaggcgacgc cggcctcagc ggagccgagt ttgaggccga gctcgtcgga gtgcatctcg
     5581 agggagaaga tgccgaccgg ccgctgcagc tcgatggcga cgtgccgggt gaggtcgagc
     5641 gccagcgacg tcttgcccat gcctggcctg ccggcgatca cgtagaggcc tcccggcagg
     5701 aagccagggc cgagccggtc ccagcccggg aagccggagg tgagcccgca gagaacctcg
     5761 gaaccgccct cttccaggtt ggcgaggaga cggttgacga cgtcgccggc gccttcccaa
     5821 tgcacgcggg cggcacggcc cagcagcttg tcggcggaac cgcggaggtc ggccagaagc
     5881 tcatggaccg gacgtgcggt cgcggtggcc gccaagaggg ttcgctgcgc gtccccgatc
     5941 aggtcacgcc ggacgcttct ctccttcaag atctcgacgt attcgtccag ccggcccagg
     6001 tccggcaggt cgaggtcgag ggcggtgaga taggccagac cgccggccgc gcccaagatc
     6061 ccggcctgct cgagatgagc ccgcagggtg aggaggtcag gagtcgagcc cgcgtcggtg
     6121 acgtcgagat aggcctggta gagcacgcgg tggcgcgcga ggtaccagtc gcgcgcatcg
     6181 agccgcctgc ggacctgggc gatgcgcagg ggctccagga ggagggcacc gaggacggcg
     6241 cgctcggctt cttcgctgtg gggaagggcc ttgtgccggg ccatcagcgc gccaggcctc
     6301 cgggtgtttc ttgcagccgg acgtcttcgc cagcgagctc gtggtggagg cggaggccgg
     6361 agagccagcg cgagagaagc acgggcggcg ccgcggtgct gggctcgagg agcagctcgg
     6421 ggaggagctg gctggtgacc agcgtgggca gccctcgctc gtcgcgggtg cgggtgagga
     6481 ggacgaggcg ttcgaggtac cagtcggccg cggcgccgga gagcggctcg tcgaggacga
     6541 ggagcggagt gaccaggagg ggcgcgagca cgtcttcggc ggcggcgaag tcggattgaa
     6601 tgcgtcgcgc cagctctggg ccggagatcc agcgtccgcg gcggccggtg cgcaggtgct
     6661 cggcgagcac gacagtcgcg aggccggtct tgccggttcc ggtcggaccc cacaaggcaa
     6721 cccagtgcgg gaggccggtc cagcgctcta gggccgcggg ccaggggcgc cggaagtggg
     6781 cggtccaact ttcgcgggtg aggcggcgat agcgctcggg gatccccgca gccgcctcgg
     6841 agaaggcgcc gagctgtgcc tcgtggcagg cgtggcacag gaggtccgcg gggaccggcc
     6901 cgaaggcgat ctggtcttcg ccgcaccggc cgcagtgcca ggacgtcact ccccggcctc
     6961 ctctcccgac gaagatctgc cggcgatgcg tgcggcctgg cggcgccgga tctcatcgac
     7021 gccggcggtg cgcaagggat tgaccgactt ggccggcctc acgggctcgg gccttgcggc
     7081 ccgctccatg accacccggt ggacgtagac gatcggcgcc cgcttgttcg ccaggccgga
     7141 ctggacgagg cggccgagca gctcgaggag cggctcgatg ccgccgaggg tcttcgacca
     7201 gagaccgatc tgcgccgggc tggggagggg aacgcccgcc tcttcgagca gcaggttgac
     7261 ccgaaggacc gcgctctgcg gccccgccag gggccggaga ccgaaggtct cctgtttttc
     7321 tgggggatta agggggatag gggggtatgg
//
LOCUS       DASPJI010000261        25156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_821009, whole genome shotgun sequence.
ACCESSION   DASPJI010000261 DASPJI010000000
VERSION     DASPJI010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 25156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25156)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25156
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821009"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VEL74_06770"
     CDS             <1..967
                     /locus_tag="VEL74_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HYG62265.1"
                     /translation="KLDKRYILASTQNHHLLNPSTEEFKTAYEEAQARHLVLTATVEE
                     FREDPEVIRKHRHVPATDESMYPNWKYEGNAWGMAIDQNVCTGCSSCVIACQAENNVP
                     VVGKEQVLAGREMHWLRIDRYFEGSLDEPMYRHQPLPCMHCETAPCEVVCPVAATVHS
                     DEGLNDMVYNRCVGTRYCSNNCPYKVRRFNFFRYSDKDTPVLSLLYNPDVTVRMRGVM
                     EKCTYCVQRIEQAKIDSKVEGEPIPANRLQTACQQACPTQAIVFGDIKDEAWEVARWR
                     KDPLSYGLLEELNTRPRTTYLAKLRNPNPALQPAEPAERTQDGHS"
     gene            954..2378
                     /gene="nrfD"
                     /locus_tag="VEL74_06775"
     CDS             954..2378
                     /gene="nrfD"
                     /locus_tag="VEL74_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HYG62266.1"
                     /translation="MATLDDGAPRAPLPKPHRPPVVAPGHTTTTVTDKISSVVLQRTP
                     KWWFFGFAVSFFLLMIFLVSVTTLFLKGVGIWGINWPIGWGFDIINFVWWIGIGHAGT
                     LISAILLLLRQQWRTSINRFAEAMTLFAVACAGMYPLLHLGRPWLFYWLLPYPNTMSL
                     WPQFRSPLIWDVFAVSTYATVSALFWFVGLIPDLATMRDRAKNKAAKIAYGMFALGWR
                     GSAKHWHNYEVASLLLAGLATPLVLSVHTVVSFDFSIGIIPGWHATIFPPYFVAGAIY
                     AGFAMVLTLAIPLRVAFGLEDFITSRHLQNMGKVMLATGLIVVYGYFCEIFFAWYSAN
                     HYERFMMLNRALGPYRFHWYMLIFCNAVVPQFLWIKKVRSSPLWLFVISMFVSVGMWL
                     ERFVIIVVSLHRDFLPASWGMYRGTIWDWGTFIGTMGLFLTLFFLFIRVLPAISVFEM
                     RTIVPGGGLGEQRDGQPIHQEVRS"
     gene            2375..2926
                     /locus_tag="VEL74_06780"
     CDS             2375..2926
                     /locus_tag="VEL74_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HYG62267.1"
                     /translation="MSALTRSRPPIYGIMAEFTSSRELIAAIRRLRQAGYRKLDAFTP
                     YPIEEVVDALDQHHTKLPLMVLIGGILGGLGGYALQYWSQVLVYPMNIGGRPVHSWPA
                     FIVPTFETTILGAALTAVFGMIALNGLPQPYHPVFNVPRFAEASRDRYFLVIEADDPK
                     FDREATRQHLADLNPFEVSEVEH"
     gene            2934..3665
                     /locus_tag="VEL74_06785"
     CDS             2934..3665
                     /locus_tag="VEL74_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYG62268.1"
                     /translation="MDRTTRRTRILLGVALAAAFVATGCRQNMHNQHKLEAYEQSEFF
                     NDQQGARPFPEGTVARGGFGEKIAPYTGLTVSVLPVSTTTQGPPVTPALVRRGQERYN
                     IFCSPCHGRLGDGRGMIVRRGYKQPTSFHDARLRGVGPDYIVSVITDGFGVMPSYAPQ
                     VPLEDRWAVAAYVRALQYSQNARLADLPDDVRGRVEAELAQPAASGGQPADGGGGGVT
                     RPAAPEEHDRRPDPGVVSGEQPGEQ"
     gene            3662..4891
                     /locus_tag="VEL74_06790"
     CDS             3662..4891
                     /locus_tag="VEL74_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62269.1"
                     /translation="MRTETVIPRIDPAAFTAPAAVKRLQSLAVVVGGIAAVLWVIGAV
                     MAPSYFLRSFLVGWVYWMGVSLGCLALSMLHHMTHGDWGIVLRRVMEAAARTLPLLAV
                     LLAVFIGALYFIGWDHLYSWASPAAANDEILQKKEAYLNVPAWVLRQILYFAIWLGLA
                     FTLSRMSLRQDRADDPGITRRMQVVAAPGLILYVLSVTFASVDWLMSLEPHWFSTIYG
                     VYLVGSQGLAALAFLIAIAVFLSRREPMSQVLQPRHFHDWGKLLLAFVMLWAYFCFSQ
                     FLITWAGNLPEEIHWFEHRLHHGWQYVALALVLFHFALPFALLLSRPLKRNAGRLIMV
                     ATVMLIMRWVDLVFQTEPSFEEQSLAFGWMYLVAPLAIGGLWLFYFLRQLQGRPLLPV
                     SDPFLPEAIAKEALAHE"
     gene            4884..5471
                     /locus_tag="VEL74_06795"
     CDS             4884..5471
                     /locus_tag="VEL74_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62270.1"
                     /translation="MSKLHIGPHTDLPHDDHANKRFDREISLRGVFWTIGGLVIVALV
                     THVLMWYLLKGIGHMEKKQDPPPLPLAAANTQAPPPEPRLQTSPEVELQSMRAEEDQA
                     LTRSSWVDQAQGTVRVPIDVAMDVIASRGLGAEVVGGQPGTNPATTPPAQQTVAPGAT
                     LQMTRPPGGAEPQAAEITSSQGQGAQASPPPPERR"
     gene            5478..6332
                     /locus_tag="VEL74_06800"
     CDS             5478..6332
                     /locus_tag="VEL74_06800"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HYG62271.1"
                     /translation="MKRIITGLTLSTLLLAPAAWALPPIPTDPGGPRVPASEMPGPLK
                     QVGFDQLLGGQVPLDAAVRDDTGRTARLGSFLGERPAVLALAYYECPMLCTLVLQGLT
                     GSLKTLSFDVGREFDVIVVSIDPGETPQMAAEKKQEIVNRYGRHGSAAGWHFLTADQQ
                     SITRIARSVGFRYYYDDQRDEYAHAAGVVVLTPQGRISRYLYGIEYAPRDMRLALIEA
                     ADAKIGSFVDQVLLYCYHYDPTIGRYSPVIMNILRLSAASAVAGLVLLIVLMRRRERR
                     VAGPVGAV"
     gene            6334..7422
                     /gene="coxB"
                     /locus_tag="VEL74_06805"
     CDS             6334..7422
                     /gene="coxB"
                     /locus_tag="VEL74_06805"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HYG62272.1"
                     /translation="MKQDLPLFPESASNFAPEVDALFWAWTAVSLFFTALIAVLIVYF
                     MVKYRRRAVDEVGQSERAATSLEIAWSVIPLIISLAMFAWGTKVYFDMFRPPSNAVEY
                     YTIGKQWMWKFQHPSGQREINTLHVPVGTPIKLNIQSEDVIHSFYVPAFRVKQDAVPG
                     RQTQIWFEATKPGVYHMFCAEYCGAEHSRMVGSVIAMEPQQYEEWLAGGPAGQSMTAS
                     GAQLFQTLACDTCHRPTTGIPARGPALEGVYGKQVRLEGGRTLIADDEYVRESILNPQ
                     AKIVAGWAPVMPTFQGQVTEEQLAQLVSYVRSLGGGGSPEAGGTAAPGGSAADSAGGQ
                     VDNPAPQTAGPAPQGTNQGPQNRPQDKT"
     gene            7429..9093
                     /gene="ctaD"
                     /locus_tag="VEL74_06810"
     CDS             7429..9093
                     /gene="ctaD"
                     /locus_tag="VEL74_06810"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HYG62273.1"
                     /translation="MDATVSPTVHPEAAVARPNYLNAEYGLKSWLLTRDHKRIAILYL
                     ISVTVFFALGALFALLVRLELLTPQGDMVQPDTYNRLFTMHGIIMVFFFLIPAIPAVL
                     GNFLVPIMIGAKDLAFPKINLASWYIYTLGGIFTLWAIVNGGVDTGWTFYTPFSTNYS
                     NTHVVMTAVGVFINGFSSIFTGLNFIVTIHRMRAPGMTWFRLPLFIWSHYATSLVMVL
                     GTPVIAITILLVAAERLLHLGIFDPRLGGDPVLFQHLFWFYSHPAVYIMILPAMGVQS
                     ELVAAFARKRIFGYRIVAFASIAIAVLGFLVWGHHMFVTGQSIYAATVFSVLSMLVAI
                     PSAVKVFNLTATLYEGSISWDTPMLYALGFIGLFTIGGLTGTMIATLGIDIHLHDTYF
                     IVAHFHYVMVGGTIMAYLGGLHYWWPKISGRLYPGFLSRLSALLVFVGFNLTFFPQFI
                     LGYLGMPRRYWSYPEEFQVFNVLSTAGATVLGVGYILPLCYFTWSMRYGKKSVDNPWG
                     AIGLEWDTPSPPPTENFYTIPVVTRGPYDYSPEEVHARRTEEVRVG"
     gene            9086..9805
                     /locus_tag="VEL74_06815"
     CDS             9086..9805
                     /locus_tag="VEL74_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HYG62274.1"
                     /translation="MASLTADSHGAHGAHAGVVHAHEHHPALQHQFDDMEQQRESSTL
                     GMWVFLVTEVMFFGGLFLAYVVYRTSFHDAFVQASNHLDPLLGFINTLVLIGSSLTMA
                     LAIWAAQVNKRKLIVWMLIATIVLGAVFLGIKAVEYTDKFTHHLVPGANFDGSDFTEP
                     RHAEIFFSLYFIMTGLHAFHMVIGIGLLAYLTIPSWKGRYDATYHTPLECTGLYWHFV
                     DLVWIFLFPLLYLLGAHLQHH"
     gene            9818..10123
                     /locus_tag="VEL74_06820"
     CDS             9818..10123
                     /locus_tag="VEL74_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HYG62275.1"
                     /translation="MAEHVSSTKSYIMVFLALAVLTVVTWAVALVDLGWANDIVAMAI
                     AVTKALLVILFFMHVRYSTRATVLTAISGFFWLAIMIFLTMNDYWTRGSSLLPVLGK"
     gene            10652..11164
                     /locus_tag="VEL74_06825"
     CDS             10652..11164
                     /locus_tag="VEL74_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62276.1"
                     /translation="MSSKLSVEEVLSNLEARAATLRGEEAFHEQQEGFHREQRELRAA
                     ELEKVLASLEAFRGAAAAAAGLALPAAARPAAASGQEKLPPPGRLMVGRLLRAVVESP
                     SLAEPFGATAVAAEANRRFSDRLPAPVGPRTASDVLRRMLAEGAIQRVREGKASQEAL
                     YRRGAPAAGS"
     gene            11262..12689
                     /locus_tag="VEL74_06830"
     CDS             11262..12689
                     /locus_tag="VEL74_06830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HYG62277.1"
                     /translation="MAVADELPHPASQPPAPDEPLSSVDYAWLRMDEPTNLMMINGVL
                     VLETPADMGRLRTVIEERLLPIPRFRQRVVLPGGGGQPRWEIDPEFDLDNHLVRVRLP
                     DPGDEAGLREVIDGLMSTPLDPERPLWQFHLIDNFAGGSVLLGRLHHCIGDGLALLMV
                     LLSLTDRTAETPHGAGANPFTSILCERGRDLHGLAHAKELVEEMMPDGLRLMLSPVEA
                     LKKTNPLLTGAGAASALGRLIGRLPDPKTAFKGPLGVPKKVAWSEALRVDDVKAVGRG
                     LGGTVNDVLLSAMTGGLRRYLEGRGERTAGINVRAAMPVNLRPLEKMAHLGNQFGLIF
                     LSLPVGIADPVERLAELGRRARALKRSAEPVVVYAILSALGKVPLAVQKLVVKIFAAK
                     TTAVMTNVPGPRQALYLAGRRIRDLFFWVPQSGRVGIGISIFSYDGHVRLGVGTDAGL
                     VPDPQRIVEAFHDEFVELQRRAGTR"
     gene            complement(12701..12961)
                     /locus_tag="VEL74_06835"
     CDS             complement(12701..12961)
                     /locus_tag="VEL74_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62278.1"
                     /translation="MRRATCVRGALLIALLALTGFAGSIPLASADPICFPGALPTCCR
                     CLNRCGAQYDTCIANATTSAQQSACYNNWYACETNCYNGPAC"
     gene            13094..15001
                     /locus_tag="VEL74_06840"
     CDS             13094..15001
                     /locus_tag="VEL74_06840"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019600052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HYG62279.1"
                     /translation="MRNRSAMRRLLLLATALGLLVTGLAVRPGTARTAKPVLHARHWL
                     AITGKPLAATAGAMMFQKGGNAVDASCAMLAATCTMWDTLGWGGETQVLIWNPKTRKV
                     IGINALGVAPTGATPELYRSKDMPYPPEFGPLAAVTPGTPGGLMVMLAEYGTLSLADV
                     LGPAIQMADGFAIEAQLANRIETEKKRIAEWPYSPATLLPHRGEAGEAREAPRPGEVF
                     RQPDLAATLRKLVEAERQALAQGKSRKEAIQAAYDRFYKGDIAEEIVRGTREGGGLFT
                     REDLANWKVKIEEPVKTTYKGIDVYKLTTWTQGPAMLQALNILEPMDLKGMGLNSSRY
                     IHTVYQAMNLAFADRDFYYGDPAFPPAEPVEGLLSKAYAEARRKQMNPERNDPAVKPG
                     DPYPFEGKTNPYLKLLETWGTDREGKEWTQPVHAGLRPNGTVSSFEEGFRAGTTSIVA
                     ADEEGWVVSATPSGGWIPAYIAGRTGIGLSQRMQSFVLTEAENPFNVLVPGKRPRVTL
                     TPSLATKDGKPYLAFAVQGGDTQDQNLLQFFLNVVEFGMNAQEAAEGPNINSYQMRSS
                     FGGHESQPGRIEVHASTPPWVRAELQRMGYTVEVEERTSGPITAIQFDHRHGTMWGGA
                     SDYGEDYGIGW"
     gene            15087..15266
                     /locus_tag="VEL74_06845"
     CDS             15087..15266
                     /locus_tag="VEL74_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62280.1"
                     /translation="MRRLILAGLVLVGALGVTQLSVPAASANHIDCSLVRCMGCDPGW
                     VFAPTPHNCCRCIPE"
     gene            complement(15335..15727)
                     /locus_tag="VEL74_06850"
     CDS             complement(15335..15727)
                     /locus_tag="VEL74_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG62281.1"
                     /translation="MIVLDASAAIEVLLQTAAGAPVAERLLMEDSSLHAPHLLDVEVA
                     QVLRRFVVKKELPAERAEQALQDLSDLPVERYSHDLLLPRAWELRHNLTAYDAVYVAL
                     AEILGATLLTRDDRISRASGHIAHVEVF"
     gene            complement(15724..15954)
                     /locus_tag="VEL74_06855"
     CDS             complement(15724..15954)
                     /locus_tag="VEL74_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62282.1"
                     /translation="MATMVQIRNVPDSMHRKLKARAALAGMSLSEYLLRELERSLDRP
                     TPTELLARLRSRTSVEPREPIAKAVAAEREGR"
     gene            complement(16042..17409)
                     /locus_tag="VEL74_06860"
     CDS             complement(16042..17409)
                     /locus_tag="VEL74_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HYG62283.1"
                     /translation="MAGIFKAYDVRGVYPTEINEDMARQIGLAFQHVLDEEDRALGNT
                     VVVSRDMRSHSEGLAQALIEGLTAAGLDVLDIGLATTPMNYFAVGHFKAAGGVQVTAS
                     HNPARYNGLKFSRHEARPVSGDHGIALMEAKVKSGDLPRADKPGTVTKGEIYDAYREH
                     VLSFLEKGDAADSGRRFKVVVDTANGMGTIYRPIIEAMGIELVPLYFELDGTFPNHEA
                     NPLKEENLHDVMRAVQEHGADLGVSFDGDADRSAFIDERGEAIGSDLITALIGGELLD
                     HEPGKAVLYDLRSSRAVHEYIEEHGGIPVRERVGHSFMKATLRKREGIFGGELAGHYY
                     FRDHYNADCAILAMVEILNLLHQTGKPLSELVAPLRRYAKSPEINFEVEDKQGKIEEL
                     ARRYSDARIDYLDGITISYDDWWANVRPSNTEPFLRLVLEARTPEELERHKHELFGML
                     GEPAE"
     gene            complement(17436..20774)
                     /gene="ileS"
                     /locus_tag="VEL74_06865"
     CDS             complement(17436..20774)
                     /gene="ileS"
                     /locus_tag="VEL74_06865"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HYG62284.1"
                     /translation="MSDKRFPDIPGGYPFPKLELGVLDEWRQGRVFERTLEQPAPRGE
                     FVFYEGPPTANNVPHVGHVVTRVIKDLFPRYRTMRGYRVPRKAGWDTHGLPVEIEVEK
                     RLGFSGKQQIEAYGIAEFNKACLDSVYTYERQWRAMTERIGYWIDLDHAYFTYTNDYI
                     ESVWWSLAELAKKGLLTEGYKIQPYCARCGTTLSSHEVAQNYKDAEDPSVWVLFPVRP
                     GQVIEPMGGGEWTVSEGLHLVAWTTTPWTVLANVALTANPDMVYRVIQHPDPERAHEQ
                     LLFGDELDKAVPLLLEIEGEKKLVDLRDQPVLARFRGRDLEGLRYDRPFQVPVEASEI
                     PVSTGGGAVSDTAGWKVVLGDYVTPAEGTGLVHTAPAFGEDDFKTGQAYGLPLLLTVE
                     ADGTIRDLPGLEPFARAWFKEADPHITRDLKRRGLLLHGDRYRHSYPFCWRCDRPLLY
                     YATTSWFIRTTVEKQALIDRNREIAWHPEHVGEGRFGNWLENLVDWALSRRRYWGTPL
                     PVWRCGECSDQTVIGSYGELFQASGREKPADVYDRGQFDPHRPYIDDFTWPCAECGSG
                     TMQRVKEVIDAWYDSGSMPFAQHHYMGEPLPHFDPRTGKGFPAAFISEAVDQTRGWFY
                     TLHALGVLLFDSVAYETCIVLGHVNDETGRKMSKRLGNVVEPMAVIEETGADALRWYF
                     CINNPEVPSRFSARLVREAAQKFLLPLWNALSFFTIYANLDGWRPGKREEVPFADRPA
                     LDRWILLRLGRLVRDVTAALDAYDTTAAARGIEEILDDLTNWYIRRSRGRFWAGNEEG
                     DGADKESAYQALYEVLTTLARLIAPFTPFVADVLHRNLVRTQSDGAAASVHLEPWPEP
                     AEGRTDEALEAGMAAIQRIVRLGHAARNLHGLKTRQPLASVTLVTADEALPGLIGPYH
                     DLLEDELNVRSIQWAADRTQYVHHEVRPIFPKTGPRFGKKMPEVKKALEAADGDALAA
                     ELETSGRITLQLSDGPAELSPEEVEVRLVEREGTATQGDRELLVALDTRLTPDLVAEG
                     WAREVVNRIQTARKELDLDYADRIRVRYRAAPELDEAIGTHRDWIAGETLAVELVAGL
                     NGEQGAAREAPVESFEFAFIIEKT"
     gene            complement(20867..21865)
                     /locus_tag="VEL74_06870"
     CDS             complement(20867..21865)
                     /locus_tag="VEL74_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYG62285.1"
                     /translation="MTGTPPRRRGRKLVWGLLLAGAAIGGPMIAGAVARRRARPPHPV
                     RWGRTRHYSGKLGEITFQELDQTPGATPLLLLHSLGPGHDADEWRAAAELLRARYHVF
                     APDLPGWGRSRGPAGGFHPAFYPEVIEEFLRRVVREPAVIVAAGLPAAFAVRVAATAP
                     ELVRGLALVAPLGITGERGGEHAGAVLPSLLGLPVLRESVLDLLTSRSALEGHLRGRV
                     YAAPERVDAALVEHHYRASHQPASRQALAVYLRGEMWTDVEEVLPGLETPVWLAWGRA
                     AAVPPVEDADLWLSRLAGSREEAGIEVFEGSGSLPHAERPAIFCRALDRFISGLPR"
     gene            22719..23276
                     /gene="def"
                     /locus_tag="VEL74_06875"
     CDS             22719..23276
                     /gene="def"
                     /locus_tag="VEL74_06875"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HYG62286.1"
                     /translation="MPVQPIVRLGHPALRTPAKPVALPQIGRPEIQQLIDDMVETMRE
                     APGVGLAAPQLGVEMQIFVYQAAGNPDRGPEIPLHVVINPMITPQSRELDYDWEGCLS
                     IPDLRGLVPRHPQVRVRGLDRDGHPLDYVATGFEARIVQHEFDHLNGVIFLDRMRDLH
                     SLSYTEEWEEYMAREGEEDEETAVG"
     gene            23291..23767
                     /locus_tag="VEL74_06880"
     CDS             23291..23767
                     /locus_tag="VEL74_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HYG62287.1"
                     /translation="MGRELVIAWAGRHHRSNWEEICDSYRKRIARWVPVRDLPVRARA
                     AADDPGRLRAEGQALLAALPQPCWIVALDSRGDSVDSDQLAERLSRVKAEWPHAIAFV
                     IGSDLGLDAALLSQARWTLSLGRLTLTHELARVVLYEQIFRALSIEAGMSYHREPL"
     gene            23826..24236
                     /locus_tag="VEL74_06885"
     CDS             23826..24236
                     /locus_tag="VEL74_06885"
                     /inference="COORDINATES: protein motif:HMM:NF044994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HYG62288.1"
                     /translation="MAKTQQAQKAPEPPGDSSQFAAIRERLLSQRQEIVDLYKQDVRA
                     GQESTDDGTEDIVDRANNSYNRELMFSLSDGERVLLLQIEDAIRRIDEGAYGRCNNCG
                     QNIALPRLQAVPWTKFCIDCQELAEKGLLSEDAS"
     gene            24301..>25156
                     /locus_tag="VEL74_06890"
     CDS             24301..>25156
                     /locus_tag="VEL74_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62289.1"
                     /translation="MADIVDKFLAGLSRQDPPQVVLVQGDLVLAVPAAERIAAVLAKT
                     GGAVVETQRHSPSLSPLIQDLRTFSLFASSKVVLAVDSAVFADREAASELIDDAADVL
                     PLKEMAGRALSSKERQAASRLFQVLRLFDVDPYAGAPERALGELPAWALEGRRSRGGK
                     PRGKKQVEELRSGLAVLLEAARGEGIQGAGEGEVAELGEVIRGGLPPGHHLVLAERVV
                     AKDHPLSVLLEERKALLRVGEVEAERGSWQGLDLLAKELERQTGVGIAPDALNELARR
                     TLRQEEGKG"
BASE COUNT         3753 a         8772 c         8561 g         4070 t
ORIGIN      
        1 caagctcgac aagcggtaca tcctggcctc cacccagaac catcacctgc tgaacccttc
       61 caccgaggag ttcaagaccg cctacgagga ggcgcaggcc cgccacctgg tcctcaccgc
      121 cacggtggag gagttccgcg aggacccgga ggtcatccgg aagcaccgcc acgtcccggc
      181 gacggacgag tcgatgtacc ccaactggaa gtacgagggg aacgcctggg gcatggcgat
      241 cgaccagaac gtctgcaccg gctgcagctc ctgtgtcatc gcctgccagg cggagaacaa
      301 cgtgccggtg gtgggcaagg agcaggtgct ggccggccgc gagatgcact ggctgcgcat
      361 cgaccgctac ttcgagggga gcctcgacga gccgatgtac cgccaccagc cgctcccctg
      421 catgcactgc gagaccgcgc cctgcgaggt ggtctgcccg gtggcggcga cggtgcacag
      481 cgacgagggg ctgaacgaca tggtgtacaa ccgctgtgtc ggcacccgct actgctcgaa
      541 caactgcccc tacaaggtgc ggcggttcaa cttcttccgg tactcggaca aggacacccc
      601 ggtcctgtcg ctgctctaca acccggacgt cacggtgcgc atgcgcggcg tgatggagaa
      661 gtgcacctac tgcgtgcagc gcatcgagca ggcgaagatc gactccaagg tcgagggcga
      721 gccgatcccg gccaaccgcc tccagaccgc ctgccagcag gcctgcccga cgcaggcgat
      781 cgtcttcggc gacatcaagg acgaggcctg ggaggtcgcc cgctggcgca aggacccgct
      841 gagctacggg ctcctcgagg agctgaacac ccggccgcgc accacctatc tggcgaagct
      901 ccggaatccc aaccccgccc tccagccggc cgagcccgcg gaaaggaccc aggatggcca
      961 ctcttgacga cggcgcgccg cgcgcgcctc tgcccaagcc tcaccggccg cccgtcgtgg
     1021 ccccgggcca cacgacgacc acggtcaccg acaagatcag ctcggtcgtc ctgcagcgga
     1081 cgcccaagtg gtggttcttc ggcttcgcgg tctcgttctt cctgttgatg atcttcctgg
     1141 tgtcggttac caccctgttt ttgaagggtg tcggcatctg gggcatcaac tggccgatcg
     1201 gctggggctt cgacatcatc aacttcgtct ggtggatcgg catcggccac gccggcacgc
     1261 tgatctcggc gatcctcctg ctgctgcgcc agcagtggcg gacgtcgatc aaccgcttcg
     1321 ccgaggcgat gacgctgttc gccgtcgcct gcgccggcat gtacccgctg ctccacctgg
     1381 ggcggccgtg gctcttctac tggctgctgc cgtaccccaa caccatgtcg ctctggccgc
     1441 agttccgcag cccgctgatc tgggacgtgt tcgcggtctc cacctacgcg acggtgtcgg
     1501 cgctgttctg gttcgtcggc ctgatccccg acctggcgac gatgcgcgac cgggcgaaga
     1561 acaaggcggc caagatcgcc tacggcatgt tcgccctcgg ctggcgcggc tcggccaagc
     1621 actggcacaa ctacgaggtg gcctcgctgc tgctcgccgg cctcgcgacg ccgctggtgc
     1681 tgtcggtcca caccgtggtg tcgttcgact tctcgatcgg catcatcccc ggctggcacg
     1741 ccaccatctt ccccccctac ttcgtcgccg gcgccatcta cgccggcttc gccatggtgc
     1801 tgacgctggc gatcccgctg cgggtcgcct tcggcctgga ggacttcatc acctcccggc
     1861 acctgcagaa catggggaag gtcatgctgg cgaccggcct catcgtcgtc tacggctact
     1921 tctgcgagat cttcttcgcc tggtacagcg ccaaccacta cgagcggttc atgatgctca
     1981 accgggcgct cggcccctac cgcttccact ggtacatgct gatcttctgc aacgccgtgg
     2041 tgccgcagtt cctgtggatc aagaaggtcc gctcgtcgcc gctctggctg ttcgtgatct
     2101 cgatgttcgt cagcgtcggc atgtggctcg agcgcttcgt catcatcgtc gtcagcctcc
     2161 accgcgactt cctgccggcg tcgtggggca tgtaccgggg gactatctgg gactggggga
     2221 cgttcatcgg cacgatgggc ctgttcctca ccctgttctt cctcttcatc cgcgtgctgc
     2281 cggcgatctc cgtgttcgag atgcggacga tcgtccccgg cggggggctc ggcgagcagc
     2341 gtgacggcca gccgattcac caggaggtgc gttcatgagc gctctcacac gctcccgccc
     2401 gccgatctac ggcatcatgg cggagttcac ctcgtcccgt gagctgatcg ccgccatccg
     2461 ccggctgcgc caggcggggt accggaagct cgacgccttc accccctatc ccatcgagga
     2521 ggtggtggac gcgctcgacc agcaccacac caagctgccg ctgatggtcc tcatcggcgg
     2581 catcctcggc gggctcggcg gctacgcgct ccagtactgg agccaggtgc tggtctatcc
     2641 gatgaacatc ggcggccggc cggtccacag ctggcccgcg ttcatcgtgc cgaccttcga
     2701 gacgaccatc ctcggcgccg cgctgaccgc ggtgttcggg atgatcgccc tcaacggcct
     2761 gccccagccg tatcacccgg tgttcaacgt gccgcgcttc gccgaggcgt cgcgcgaccg
     2821 gtatttcctg gtgatcgagg ccgacgatcc gaagttcgac cgcgaggcca cccgccagca
     2881 cctggcggac ctcaatccct tcgaggtgtc cgaagttgag cactgaggcg accatggacc
     2941 ggaccacccg acggacacgg atcctgctgg gcgtggcgct ggccgcggcc ttcgtggcca
     3001 ccggctgccg gcagaacatg cacaaccagc acaagctcga ggcctacgag cagagcgagt
     3061 tcttcaacga ccagcagggc gcccgcccgt tccccgaggg gacggtggcg cgcggcggct
     3121 tcggcgagaa gatcgcgccc tacaccggcc tcacggtcag cgtcctgccg gtatcgacca
     3181 cgacccaggg gccgccggtg acgccggcgc tcgtccgccg cggccaggag cggtacaaca
     3241 tcttctgctc cccctgccac ggccggctgg gcgacggccg cggcatgatc gtccgccgcg
     3301 gctacaagca gccgacgtcg ttccatgacg cgcggctgcg cggcgtcggc cccgactaca
     3361 tcgtcagcgt tatcaccgat ggcttcggcg tgatgccgag ctacgcgccg caggtgccgc
     3421 tggaggaccg ctgggccgtc gccgcctatg tgcgcgccct gcagtacagc cagaacgccc
     3481 gcctggcgga cctgccggac gacgtgcgcg gccgggtcga ggccgagctg gcgcagccgg
     3541 cggcctccgg cgggcagccg gcggacggtg gcggcggcgg cgtgacccgt cccgccgcgc
     3601 cggaggagca cgaccgccgg cccgatccgg gagtggtgtc aggagaacaa ccgggagagc
     3661 aatgagaact gagaccgtca tccctcgaat cgatccggcg gcgttcaccg ctccggccgc
     3721 ggtcaagcgc ctccagtcgc tggccgtggt ggtcggcggc atcgcggccg ttctctgggt
     3781 gatcggcgcg gtcatggcgc cgtcctactt cctgcgctcc ttcctggtcg gctgggtgta
     3841 ctggatgggg gtctcgctgg gctgcctcgc cctgtcgatg ctccatcaca tgacgcacgg
     3901 cgactggggg atcgtgctgc ggcgggtgat ggaggcggcg gcgcgcacgc tgccgctcct
     3961 cgccgtgctg ctcgccgtgt tcatcggcgc cctctacttc atcggctggg accacctcta
     4021 ctcctgggcg tcgccggcgg ccgccaacga cgagatcctg cagaagaagg aggcctacct
     4081 caacgtcccg gcctgggtgc tgcgccagat cctctacttc gcgatctggc tcggcctcgc
     4141 cttcacgctc agccgcatgt cgctgcgcca ggaccgcgcg gacgatcccg gcatcacgcg
     4201 gcgcatgcag gtggtggcgg cgccggggct catcctctac gtgctgtcgg tgacgttcgc
     4261 ctcggtggac tggctgatgt cgctcgagcc gcactggttc tcgaccatct acggcgtcta
     4321 cctcgtcggc agccagggcc tggcggcgct cgccttcctc atcgccatcg cggtcttcct
     4381 gtcgcgccgc gagccgatgt cgcaggtatt gcagccgcgg cacttccacg actggggcaa
     4441 gctgctgctg gccttcgtca tgctctgggc ctacttctgc ttctcgcagt tcctgatcac
     4501 ctgggcgggc aacctgccgg aggagatcca ctggttcgag caccgtctgc accacggctg
     4561 gcagtacgtg gcgctggcgc tggtgctctt ccacttcgcg ctgccgttcg cgctgctgct
     4621 gtcgcgtccg ctgaagcgca acgccggccg gctgatcatg gtcgccaccg tgatgctgat
     4681 catgcggtgg gtcgacctgg tgttccagac cgagccgtcg ttcgaggagc agagcctggc
     4741 cttcggctgg atgtacctgg tggcgccgct cgccatcggc ggcctctggc tgttctactt
     4801 cctcaggcag ctccagggcc ggccgctgct gccggtgagc gaccccttcc tgccggaggc
     4861 gatcgccaag gaggcgctgg cccatgagta agctccacat cggccctcac accgatctgc
     4921 cgcacgacga tcacgcgaac aagcgcttcg accgcgagat cagcctgcgc ggcgtcttct
     4981 ggaccatcgg cggtctggtc atcgtggcgc tggtgacgca tgtcctcatg tggtacctgc
     5041 tcaaggggat cggtcacatg gagaagaagc aggacccgcc gccgctgccg ctggcggcgg
     5101 ccaacaccca ggcgccgccg ccggagccgc ggctccagac ctcgccggag gtggagctgc
     5161 agtcgatgcg ggcggaggag gaccaggcgc tcacccgctc gtcctgggtg gaccaggccc
     5221 agggcacggt ccgcgtgccc atcgacgtgg cgatggacgt catcgccagc cgcggcctcg
     5281 gcgccgaggt ggtgggcggc cagccgggga ccaacccggc gaccacgcca ccggcgcagc
     5341 agaccgtggc gcccggggcc accctgcaga tgacccggcc gccgggcggc gcggagccgc
     5401 aggccgccga gatcacgagc tcacaggggc agggggcgca ggcctctccg cccccaccgg
     5461 agagaaggta agccgtcttg aagcgaatca tcacgggttt gaccctttcc accctgctgc
     5521 tcgccccggc cgcctgggcg ctgcccccca tccccacgga tccgggcggg ccgcgcgtcc
     5581 cggcgtccga gatgccgggg ccgctcaagc aggtgggctt cgaccagctc ctcggcggcc
     5641 aggtgccgct cgatgccgcc gtgcgcgacg acaccgggcg gacggcgcgg ctggggagct
     5701 tcctcggtga gcgcccggcc gtcctggcgc tcgcctacta cgagtgcccg atgctctgca
     5761 ccctggtgct ccaggggctg accgggagcc tcaagaccct gtcgttcgac gtcggccgcg
     5821 agttcgacgt catcgtggtg agcatcgatc ccggcgagac gccgcagatg gcggccgaga
     5881 agaagcagga gatcgtcaac cgctacggcc gccacggcag cgcggcgggg tggcacttcc
     5941 tcaccgccga ccagcagtcg atcacccgga tcgcccggtc ggtgggcttc cgttactact
     6001 acgacgacca gcgggacgag tatgcccacg ccgcgggcgt ggtggtgttg acgccgcagg
     6061 ggcggatctc ccgctacctg tacggcatcg agtacgcgcc gcgggacatg cgcctggcgc
     6121 tcatcgaggc ggcggacgcc aagatcggct ccttcgtcga ccaggtgctc ctgtattgct
     6181 atcactacga cccgacgatc ggtcggtaca gtcccgtcat catgaacatc ttgcgcctgt
     6241 cggcggcgtc ggccgtcgcg ggcctggtcc tcctgatcgt gctgatgcgg cggcgggagc
     6301 gccgtgtggc gggtccggtg ggggcggtct gagatgaagc aagatcttcc tctgtttccc
     6361 gagtcggcct cgaacttcgc tcccgaggtg gacgccctgt tctgggcctg gaccgcggtc
     6421 agcctgttct tcaccgcgct catcgccgtg ctcatcgtct acttcatggt caagtaccgg
     6481 cggcgggcgg tggacgaggt gggccagagc gagcgcgcgg cgacgtcgct cgagatcgcc
     6541 tggtcggtga tcccgctgat catcagcctg gcgatgttcg cctggggcac caaggtctac
     6601 ttcgacatgt tccggccgcc atccaacgcg gtcgagtact acaccatcgg caagcagtgg
     6661 atgtggaagt tccagcatcc gagcggccag cgcgagatca acacgctgca cgtgccggtg
     6721 ggcacgccga tcaagttgaa catccagtcc gaggacgtca tccatagctt ctacgtcccg
     6781 gccttccggg tgaagcagga cgcggtgccg ggccggcaga cgcagatctg gttcgaggcc
     6841 accaagcccg gggtttacca tatgttctgc gccgagtatt gcggtgcgga gcactcccgg
     6901 atggtcggca gcgtcatcgc catggagccc cagcagtacg aggagtggct cgccggcggt
     6961 ccggcggggc agtcgatgac cgcctcgggc gcgcagctct tccagactct ggcctgcgac
     7021 acctgccacc ggccgacgac cgggatcccg gcgcgcggcc cggctctcga gggggtctat
     7081 ggcaagcagg tgcggctcga gggcgggcgc accctcatcg ccgacgacga gtacgtccgc
     7141 gagtcgatcc tgaacccgca ggcgaagatc gtggccggct gggcgccggt catgcctacc
     7201 ttccaggggc aggtcaccga ggagcagctc gcgcagctcg tctcctacgt ccgctcgctg
     7261 ggcgggggag gcagccccga ggccggcggc accgcggcgc cgggcggctc cgccgccgac
     7321 agcgcgggcg gccaggtgga caatccggct ccgcagaccg cgggccccgc cccccagggg
     7381 acgaaccagg gaccgcagaa ccgtccgcag gacaaaacgt aaggacttat ggacgccacc
     7441 gtcagcccca ccgttcatcc cgaggccgcc gtcgcgcggc ccaactacct caatgccgag
     7501 tacggcttga agagttggct gctgacacgg gaccacaaga ggatcgccat cctctatctg
     7561 atctcggtca ccgtcttctt cgccctgggg gccctgttcg ccctgctggt ccgcctggag
     7621 ctgctgacgc cccagggcga catggtgcag ccggatacct acaaccggct cttcaccatg
     7681 cacggcatca tcatggtctt cttcttcctg atcccggcga tcccggcggt gctggggaac
     7741 ttcctcgtgc cgatcatgat cggggcgaag gacctggcgt tccccaagat caacctggcg
     7801 agctggtaca tctacaccct gggcggcatc ttcaccctgt gggccatcgt caacggcggt
     7861 gtggacaccg gctggacctt ctacacgccg ttctcgacca actactccaa cacccacgtg
     7921 gtgatgacgg cggtcggtgt cttcatcaac ggcttctcgt cgatcttcac cgggctgaac
     7981 ttcatcgtca ccatccaccg gatgcgggcg ccgggcatga cctggttccg cctgccgctg
     8041 ttcatctggt cgcactacgc gaccagcctg gtgatggtgc tcggcacgcc ggtgatcgcg
     8101 atcaccatcc tcctggtggc ggccgagcgg ctgctgcacc tggggatctt cgacccgcgg
     8161 ctgggtggcg acccggtcct cttccagcac ctgttctggt tctactccca cccggcggtc
     8221 tacatcatga tcctgccggc gatgggggtg cagagcgagc tggtggcggc gttcgcccgc
     8281 aagcgcatct tcggctaccg catcgtcgcc ttcgccagca tcgccatcgc ggtgctcggc
     8341 ttcctggtct ggggccacca catgttcgtc accggccagt cgatctacgc cgcgacggtc
     8401 ttctcggtgc tctcgatgct ggtggccatc ccgtcggccg tcaaggtgtt caacttgacc
     8461 gcgacgctct acgagggctc gatctcctgg gacacgccga tgctctacgc cctgggcttc
     8521 atcggcctgt ttaccatcgg cggcctgacc ggcacgatga tcgcgacgct gggcatcgac
     8581 atccacctgc acgacaccta cttcatcgtg gcgcacttcc actacgtcat ggtgggcggc
     8641 acgatcatgg cctacctggg cgggctccac tactggtggc cgaagatctc gggccggctc
     8701 tatccggggt tcctgtcgcg cctgtcggcg ctgctggtct tcgtcggctt caacctgacg
     8761 ttcttcccgc agttcatcct gggttacctg gggatgccgc ggcggtactg gtcgtacccc
     8821 gaggagttcc aggtgttcaa cgtcctgtcg acggctggcg ccacggtgct gggggtgggc
     8881 tacatcctgc cgctctgcta cttcacctgg tcgatgcgct acggcaagaa gagcgtcgac
     8941 aacccctggg gggccatcgg cctcgagtgg gacacgccct cgccgccgcc caccgagaac
     9001 ttctacacca tcccggtggt gacccgcggc ccctacgact attcgcccga ggaggtccac
     9061 gcccggcgga ccgaggaggt gcgcgttggc tagcttgacg gccgattcac acggcgccca
     9121 cggcgctcac gcgggggtgg tgcatgccca tgagcaccac cccgccctgc agcaccagtt
     9181 cgacgacatg gagcagcagc gcgagtcgtc gacgctgggc atgtgggtct tcctggtgac
     9241 ggaggtcatg ttcttcggcg ggctgttcct cgcctacgtg gtctaccgta cgtcgttcca
     9301 cgacgcgttc gtccaggcga gcaatcacct ggacccgctg ctcgggttca tcaacacgct
     9361 ggtgctgatc ggcagcagct tgaccatggc gctggccatc tgggcggcgc aggtgaacaa
     9421 gcgcaagctg atcgtctgga tgctgatcgc gaccatcgtc cttggcgccg tgttcctggg
     9481 catcaaggcg gtcgagtaca cggacaagtt cacgcaccac ctggtgccgg gcgccaattt
     9541 cgacggctcc gacttcaccg agccgcggca cgccgagatc ttcttctcgc tctacttcat
     9601 catgaccggg ctccacgcct tccacatggt gatcggcatc gggctgctgg cgtacctgac
     9661 catcccgtcc tggaaggggc ggtacgacgc cacgtaccac actccgctgg agtgcacggg
     9721 cctctactgg cacttcgtgg acctcgtctg gatcttcctc ttcccgttgc tctatctgct
     9781 gggcgcgcac ctgcagcatc actgatacga ggacaacatg gccgagcacg tttcctccac
     9841 caagtcgtac atcatggtct tcctggccct ggccgtcctc acggtcgtca cctgggccgt
     9901 cgctctggtc gacctgggct gggcgaacga catcgtcgcg atggcgatcg ccgtgaccaa
     9961 ggcgttgctg gtgatcctgt tcttcatgca cgtccgctac agcacgcgcg cgacggtcct
    10021 gacggccatc tccggcttct tctggctggc gatcatgatc ttcctgacga tgaacgacta
    10081 ctggacccgg ggcagctcgc tgctgcccgt cctgggcaag taagagtccg caagaacgtc
    10141 tccggcgccc cccgttcggg gggcgccggc gcttcagggg agctcgttcg tcgagaagcc
    10201 tcaccgcgac tccagaagct tcgccgcaag ctcgatctct ctgcagcaac tccttttgcc
    10261 tcaccgcgac ttccttcggt ctgccgcgac gtagctccgt tcgccgcaag ctcaagagat
    10321 tcaccgcaag atcggtccgt tcgccgcgac tttgttggat tcaccgcgac ttcaaataac
    10381 tcgccgcaac ttcgtttgat tcaccgcaag ctcttcccgt tcatcgcaag ctgttcccgt
    10441 tcaccgcaag ccgagagcct tcaccgcaac ctcgaaggac tggccgcaag ttccaggaag
    10501 tcaccgcgac cttgccagct tcgccgcgac ttcaagtgaa aatggctcgg tggagtgtag
    10561 aatcctcccc cagtcgccgg ttcaccgcaa tccgccgcga tccgccgcaa gatcggccgg
    10621 cgcctgccgt cccaagaggg ggagacctga catgagctcg aagctgtcgg tggaagaggt
    10681 gttgtccaat ctggaagcgc gcgcggccac cctgcgcggc gaggaggcgt tccacgagca
    10741 gcaggaaggt ttccatcgag agcagcggga gctgcgcgcc gccgagttgg agaaggtgct
    10801 ggcgagcctg gaggccttcc gcggcgccgc cgcggccgcc gccggcctcg ccctgccggc
    10861 tgccgcccgg cccgccgccg cgagcgggca ggagaagctg ccaccaccgg gccgcctgat
    10921 ggtcggccgg ttgctccggg cagtcgtcga gagcccctcg ctcgccgagc ctttcggcgc
    10981 caccgccgtc gccgcggaag ccaaccgccg cttctcggac cgcttgccgg cccccgtcgg
    11041 cccgcggacc gcttcggacg tcctgcgccg gatgctcgcg gagggcgcga tccagcgggt
    11101 tcgtgagggc aaggcctccc aggaggcgct ctatcgccgc ggtgcgccag cggcggggag
    11161 ctgagccggg gtccgcgcgg agaccttcga gattctcaga cgtatacttt gacccgatgc
    11221 cgccgggcgc cggcggcggt ccgtccgatc gaggaagatc cttggccgtc gccgacgagc
    11281 tcccgcaccc cgcctcccag ccgccggctc ccgacgagcc gctgtcgagc gtcgactacg
    11341 cgtggctgcg gatggacgag ccgaccaacc tcatgatgat caacggcgtt ctcgtgctcg
    11401 agactccggc cgacatgggg cggctgcgga ccgtgatcga ggagcggctt ctccccatcc
    11461 cgcgcttccg tcagcgggtg gtgctccccg gcgggggagg gcagccgcgg tgggagatcg
    11521 atcccgagtt cgacctcgac aaccatctgg tgcgcgtgcg gctgccggac cccggggacg
    11581 aggcgggcct ccgcgaggtg atcgacgggc tgatgagcac gccgctcgat cccgagcgcc
    11641 cgctctggca gttccacctc atcgacaact tcgcgggcgg ctccgtcctt ctcgggcggc
    11701 tgcaccattg catcggtgac ggcctggccc tgctgatggt cctgctgtcg ttgaccgacc
    11761 gcaccgccga gacgccgcac ggggccggag ccaacccgtt cacgtcgatc ctctgcgagc
    11821 gagggcgcga cctccacggc ctcgctcatg cgaaggagct ggtcgaagag atgatgccgg
    11881 acggcctccg cttgatgctg agcccggtgg aggcgctcaa gaagacgaac cccctgctga
    11941 ccggcgccgg tgccgcgagc gctctcggcc gcctcattgg ccggctgccg gacccgaaga
    12001 ccgccttcaa gggcccgctg ggggtgccca agaaggtcgc ctggtccgag gcgctccggg
    12061 tcgacgacgt caaggcggtg ggccgtggcc tcgggggcac ggtcaacgac gtgctgctct
    12121 cggcgatgac cggcgggctg cgccgctatc tggaagggcg cggcgagcgg acggccggga
    12181 tcaacgtgcg cgcggccatg ccggtgaacc tccggccgct cgagaagatg gcgcacctgg
    12241 gcaatcagtt cggcctgatc ttcctgtcgc tgccggtggg gatcgccgat ccggtggagc
    12301 ggctggcgga gctcggccgc cgcgcccggg cgctcaagcg ctcggccgag ccggtggtgg
    12361 tctacgccat cctgtcggcg ctcggcaagg tgccgctggc cgtccagaag ctggtggtga
    12421 agatcttcgc cgccaagacc acggcggtga tgaccaacgt cccggggccg cggcaggcgc
    12481 tctacctcgc cggccgtcgc atccgtgacc tcttcttctg ggtcccgcag tcagggcggg
    12541 tggggatcgg catcagtatc ttcagctacg acggccacgt ccgcctcggc gtcggcaccg
    12601 acgccggcct cgtccccgac ccgcagcgca tcgtcgaagc cttccacgac gagttcgtcg
    12661 agctgcagcg gcgggccggg acgaggtagg gagccgttgc tcagcaggcg gggccgttat
    12721 agcaattcgt ctcgcaggcg taccagttgt tgtagcaggc cgactgctgg gcgctggtcg
    12781 tcgcattggc gatgcaggtg tcgtactggg ctccgcaccg gttgaggcac cggcagcagg
    12841 tggggagggc gccggggaag cagatggggt cggccgaggc cagggggatg ctgcccgcga
    12901 agccggtcaa ggcgagcagg gcgatcaaca gggctccacg gacacaggtc gctcttctca
    12961 ttcgatctcc tccttcgaaa ggaggagctt agtcttttcg gtaccgctcg gccagactgc
    13021 gcctcggccc atccggcgct atgcttaccg gctcgggcgc ggctcggcgc gcccgctgga
    13081 ggaggaatcg aacatgcgga atcgctctgc gatgcggcgg ctgcttctac tcgctacggc
    13141 cctcgggctc ctggtgacgg gcctggccgt ccggccgggg accgcgcgca ccgccaagcc
    13201 cgtgctccac gcccgccact ggctggcgat caccggcaag ccgctggcgg cgaccgccgg
    13261 cgccatgatg ttccagaagg ggggcaacgc cgtggacgcc tcctgcgcca tgctcgccgc
    13321 cacctgcacc atgtgggaca ccctggggtg gggcggcgag acgcaggtcc tgatctggaa
    13381 cccgaagacc aggaaggtca tcggcatcaa cgcgctgggc gtcgccccca ccggcgccac
    13441 ccccgagctc taccggagca aggacatgcc ctacccgccg gagttcgggc cgctcgcggc
    13501 ggtgacgccg ggcacccccg gcggcctgat ggtgatgctg gccgagtacg gcaccctgtc
    13561 gctcgccgac gtgctcggcc cggcgatcca gatggcggac ggcttcgcca tcgaggccca
    13621 gctcgcgaac cgtatcgaga ccgagaagaa gcgcatcgcc gagtggccct actcgccggc
    13681 cacgctgctg ccgcatcgtg gagaggcggg cgaagcgcgc gaggcgccgc ggcccggcga
    13741 ggtgttccgt cagccggacc tcgccgccac cctgcgcaag ctggtggagg ccgagaggca
    13801 ggcgctggcc caaggcaaga gccgcaagga ggcgatccag gccgcctatg accggttcta
    13861 caagggggac atcgccgagg agatcgtccg cggcacccgc gaggggggcg gcctcttcac
    13921 ccgcgaggac ctggccaact ggaaggtgaa gatcgaggag ccggtcaaga ccacctacaa
    13981 ggggatcgac gtctacaagc tgaccacctg gacgcagggg ccggccatgc tccaggcgct
    14041 gaacatcctg gagccgatgg atttgaaagg catggggctc aacagctctc gctacatcca
    14101 cacggtctac caggcgatga acctggcctt cgccgaccgc gacttctact acggcgaccc
    14161 ggccttcccg cccgccgagc cggtcgaggg gctgctctcc aaggcctacg ccgaggcgcg
    14221 ccggaagcag atgaacccgg agcgcaacga tccggcggtc aagccgggcg atccctatcc
    14281 gttcgagggg aagaccaacc cctacctgaa gctgctcgag acctggggga ccgaccggga
    14341 ggggaaggag tggacgcagc cggtgcacgc cggcctccgt ccgaacggca ccgtctccag
    14401 cttcgaggag ggcttccgcg ccggcaccac gtcgatcgtc gccgccgacg aggaggggtg
    14461 ggtggtgtcc gccacgccga gcggcggctg gattccggcc tacatcgccg gccgtaccgg
    14521 catcggcctg tcgcagcgca tgcagagctt cgtgctcacc gaggcggaga atccgttcaa
    14581 cgtcctggtg ccgggcaagc gcccgcgggt gaccctgacg cccagcctgg cgacgaagga
    14641 cggcaagccc tacctcgcct tcgcggtgca ggggggcgac acccaggacc agaacctcct
    14701 ccagttcttc ctcaacgtgg tcgagttcgg gatgaacgcg caggaggcgg ccgaggggcc
    14761 gaacatcaac agctaccaga tgcgcagctc cttcggcggc cacgagtcgc agcccggccg
    14821 gatcgaggtc cacgccagca caccgccgtg ggtgcgggcc gagctccaga ggatgggcta
    14881 cacggtggag gtcgaggagc ggacctcggg cccgatcaca gccattcaat tcgaccaccg
    14941 gcacggcacg atgtgggggg gcgccagcga ctacggcgag gactatggga tcggctggta
    15001 ggctgtcccc cttcgggagc cgtcctacac cttatagttg aaccagaaag tcttctgagg
    15061 ctttcatgtc tcccgaagga ggatgtatgc ggagactgat tctcgcaggt ctcgtgctcg
    15121 tcggcgccct gggcgtcacc cagctctcgg tcccggccgc cagcgcaaac cacatcgact
    15181 gctcgctcgt ccgctgcatg ggctgcgacc cgggttgggt gttcgcgccc accccgcaca
    15241 actgctgccg ctgcatcccg gagtagctcc cgtccaagac accggccgcg ccctgtcggg
    15301 cgtggccggg tggtacccct agctacagcc agtcttagaa gacttcgacg tgggctatgt
    15361 gaccgctggc ccgagagata cggtcgtcgc gggtgagaag ggttgctccg aggatctcgg
    15421 cgagggccac atagacggcg tcgtaggcgg tgaggttgtg cctcagctcc caggcccgcg
    15481 ggagcaggag gtcatgggag taacgctcga ccgggagatc ggagaggtcc tgcagggcct
    15541 gttcggcccg ttccgccggg agctccttct tcacgacgaa ccgtcgcaga acctgggcga
    15601 cctcgacgtc gaggagatga ggagcatgga gcgacgaatc ctccatcagc aggcgctcgg
    15661 cgaccggagc ccctgccgcc gtctgcaaga gcacttcgat cgcggcggag gcgtccagaa
    15721 cgatcatcgg ccttcgcgtt cggccgcgac cgccttggcg atcggctctc gtggctccac
    15781 gctggtacgg ctccggagcc ttgcgaggag ctccgtcggc gtcggccgat ccagggatcg
    15841 ctcgagctcg cggagcagat actccgacag agacatcccc gcgagtgcgg cccgggcctt
    15901 cagcttccgg tgcatggagt cagggacgtt gcggatctga accatcgtag ccatgcgcgc
    15961 agtctatttg catgcatagc acatgtcaaa ggtgggagcc gtatcgagtc ccggctcccg
    16021 atcggaccga tccaattaag gctactccgc cggctcgccc agcatcccga acagctcgtg
    16081 cttgtgccgc tccagctcct cgggggtgcg ggcttcgagg accaggcgca ggaagggctc
    16141 ggtgttcgag gggcggacgt tggcccacca gtcgtcgtag ctgatggtga tcccgtcgag
    16201 atagtcgatc ctggcgtcgg agtagcggcg ggccagctct tcgatcttgc cctgcttgtc
    16261 ctccacctcg aagttgatct ccgggctctt ggcgtagcgg cgcagcggcg ccaccagctc
    16321 ggagagcggc ttgccggtct ggtgcaggag gttcaggatt tcgaccatcg ccaggatcgc
    16381 gcagtcggcg ttgtagtggt cccggaagta gtagtggccc gccagctcgc cgccgaagat
    16441 cccctcccgc ttgcgcaacg tcgccttcat gaaggagtgg cccacccgct cgcgcaccgg
    16501 gatgccgccg tgctcctcga tgtactcgtg caccgcccgc gatgagcgca ggtcgtagag
    16561 gaccgccttg cccggctcat ggtccagcag ctcgccgccg atcagagccg tgatcaggtc
    16621 gctcccgatc gcctcgccgc gctcgtcgat gaaggccgag cggtcggcgt cgccgtcgaa
    16681 ggacaccccc aggtcggcgc cgtgctcctg gaccgcccgc atcacatcgt ggaggttctc
    16741 ctccttgagc ggattggcct cgtgattcgg gaaggtgccg tcgagctcga aatagagggg
    16801 caccagctcg atccccatcg cctcgatgat cggccggtag atggtcccca tgccgttggc
    16861 ggtgtcgacc accaccttga agcgccgtcc gctatcggcg gcatccccct tctccaggaa
    16921 ggacaggacg tgctcgcggt aggcgtcgta gatctccccc ttggtgaccg tgcccggctt
    16981 gtccgctcgc ggcaggtcgc cggacttcac cttggcttcc atcagggcga tgccgtggtc
    17041 ccccgacacc ggccgcgcct cgtggcggct gaacttgagg ccgttgtagc gcgccgggtt
    17101 gtggctggcc gtcacctgca cgccgcccgc cgccttgaag tggccgacgg cgaagtagtt
    17161 catcggcgtg gtggcgaggc cgatgtccag cacgtcgagg ccggccgcgg tcagcccctc
    17221 gatcagggcc tgtgccagac cctcgctgtg cgagcgcatg tcgcggctca ccaccaccgt
    17281 attgcccagc gcgcggtcct cctcgtccag cacgtgctgg aaggcgaggc cgatctggcg
    17341 cgccatgtcc tcgttgatct ccgtgggata gaccccgcgg acgtcataag ccttgaagat
    17401 tccggccatc tgctctctcc ctctccgtat ctcgatcagg tcttctcgat gatgaaagcg
    17461 aactcgaagc tctcgaccgg cgcctcgcgc gccgctccct gctctccgtt gagccccgcg
    17521 accagctcga ccgccagcgt ctcgccggcg atccagtcgc ggtgggtgcc gatggcctcg
    17581 tccagttccg gcgcggcgcg gtagcggacg cggatgcggt ccgcgtagtc caggtccagc
    17641 tccttgcggg cggtctggat gcggttgacc acctcgcgcg cccacccctc ggccaccagg
    17701 tccggagtga ggcgcgtgtc cagggcgact agcagctcgc ggtctccctg tgtcgccgtg
    17761 ccctcacgct cgaccagccg gacctccacc tcctcgggcg acagctcggc cggaccgtcc
    17821 gagagctgga gggtgatccg gccgctcgtc tccagctccg cggccagcgc gtcaccgtcc
    17881 gccgcctcca gggccttctt gacctccggc atcttcttgc cgaagcgcgg gccggtcttg
    17941 gggaagatcg gccgcacctc gtgatggacg tactgggtcc ggtcggccgc ccactggatc
    18001 gagcggacgt tgagctcgtc ttccaagagg tcgtgatagg ggccgatgag gccgggcagc
    18061 gcctcgtcgg cggtcaccag ggtgacggag gccagcggct gccgggtctt caagccgtgg
    18121 aggttgcgcg ccgcgtgccc cagccgcacg atgcgctgga tggcggccat gccggcctcg
    18181 agcgcctcgt cggtccgccc ctcggccggc tcgggccagg gctccaggtg gacgctcgcc
    18241 gcggcaccgt cactctgggt gcggaccagg ttgcggtgca gcacgtcggc gacgaacggg
    18301 gtgaaggggg cgatgagccg cgccagcgtg gtcagcacct cgtacagcgc ctggtacgcg
    18361 ctctccttgt cggcgccgtc tccctcctcg ttccctgccc agaagcggcc gcggctgcgc
    18421 cggatgtacc agttggtcag gtcgtcgagg atctcctcga tcccgcgcgc ggcggccgtg
    18481 gtgtcgtagg cgtccagggc ggccgtgacg tcgcgcacca ggcggccgag gcgcagcagg
    18541 atccagcggt ccagcgccgg ccgatccgcg aaggggacct cctcgcgttt ccccggacgc
    18601 cagccgtcga ggttggcgta gatggtgaag aacgacagcg cgttccacag ggggaggagg
    18661 aacttctggg ccgcctcgcg caccagccgc gccgagaagc gcgacggcac ctccgggttg
    18721 ttgatgcaga aataccagcg tagcgcgtcc gccccggtct cctcgatcac ggccatgggc
    18781 tcgacgacgt tgcccaggcg cttcgacatc ttgcggccgg tctcgtcgtt gacgtgcccg
    18841 agaacgatgc aggtctcgta ggcgaccgaa tcgaacagca ggacgcccag cgcgtgcagg
    18901 gtgtagaacc agccgcgcgt ctggtcgacg gcctcgctga tgaacgccgc cgggaacccc
    18961 ttgcccgtcc gcgggtcgaa gtgcggcagc ggctcgccca tgtagtggtg ctgggcgaac
    19021 ggcatggaac ccgagtcgta ccaggcgtcg atgacctcct tgacgcgctg catggtgccg
    19081 gacccgcact ccgcgcacgg ccaggtgaag tcgtcgatgt acggccggtg cgggtcgaac
    19141 tgcccgcggt cgtagacgtc ggcgggcttc tcccggccgc tcgcctggaa gagctcgccg
    19201 tagctgccga tcaccgtctg gtcgctacac tcgccgcagc gccagaccgg cagcggcgtc
    19261 ccccagtagc ggcggcgcga cagggcccag tccaccaggt tctccagcca gttgccgaag
    19321 cgcccctcgc cgacgtgctc ggggtgccag gcgatctcgc ggttcctgtc gatcagcgcc
    19381 tgcttctcga cggtggtgcg gatgaaccag ctcgtcgtcg cgtaatagag gagcggccgg
    19441 tcgcagcgcc agcagaacgg gtagctgtgg cgatagcggt cgccgtgcag cagcaggccg
    19501 cggcgcttga gatcgcgggt gatgtgcggg tccgcctcct tgaaccaggc gcgggcgaag
    19561 ggctcgaggc cgggcaggtc gcggatggtg ccgtccgcct ccacggtgag cagcaggggg
    19621 aggccgtagg cctggccggt cttgaagtcg tcctcgccga aggccggcgc ggtgtggacc
    19681 agaccggtgc cctcggccgg cgtcacgtaa tctccgagga ccaccttcca gccggcggtg
    19741 tccgagacgg cgccccctcc ggtagacacc gggatctccg acgcctccac cggcacctgg
    19801 aacggccggt cgtagcgcaa cccttcgagg tcgcggccgc ggaagcgggc gaggacgggc
    19861 tggtcccgca ggtcgaccag cttcttctcc ccctcgatct cgagcagcag ggggacggcc
    19921 ttgtcgagct cgtcgccgaa caggagctgc tcgtgggcgc gctcggggtc cgggtgctgg
    19981 atcacgcggt agaccatgtc cggattggcg gtgagggcca cgttggcgag caccgtccac
    20041 ggggtggtgg tccaggcgac gagatggagc ccctcgctca ccgtccactc gccgccgccc
    20101 atgggctcga tcacctggcc ggggcggacg gggaagagca cccacacgga ggggtcctcg
    20161 gcgtccttat agttctgggc cacctcgtgg ctggagaggg tggtgccgca gcgggcgcaa
    20221 tagggctgga tcttgtagcc ctcggtcagc agccccttct tcgccagctc ggcgagcgac
    20281 caccagaccg actcgatgta gtcgttggtg taggtgaagt aggcgtggtc gaggtcgatc
    20341 cagtagccga tgcgctcggt catggcgcgc cactggcgct cgtaggtata gacgctgtcg
    20401 aggcaggcct tattgaactc ggcgatgccg taggcctcga tctgctgctt gccggagaag
    20461 ccgaggcgct tctcgacctc gatctcgacc ggcaggccgt gggtgtccca gcctgccttg
    20521 cgcggcacgc ggtagccgcg catggtgcgg tagcgcggga agaggtcctt gatgacccgg
    20581 gtcaccacgt ggccgacgtg cgggacgttg ttggccgtgg gcgggccctc gtagaagacg
    20641 aactcgcccc ggggcgccgg ctgctccagc gtccgctcga agacgcgccc ctgccgccac
    20701 tcgtcgagca cgccgagctc gagcttcggg aaggggtaac ccccgggaat atcgggaaat
    20761 ctcttgtcgc tcatgatgaa aggaccctct cgcctgccgc cggccggcca gccggacagc
    20821 cggggcaggg gcgctattgt aaccgccggg tcaagagggg ggcggatcag cggggcaggc
    20881 cggagatgaa gcggtcgagg gcgcggcaga agatggcggg ccgctcggcg tgggggaggc
    20941 tgccgctccc ctcgaagacc tcgatcccgg cctcttcccg ggaccccgcg agccggctca
    21001 gccagaggtc ggcgtcctcg accggcggca cggcggcagc ccggccccag gcgagccaga
    21061 ccggcgtctc cagcccgggg aggacctcct cgacgtcggt ccacatctcg ccgcgcaggt
    21121 agacggcgag ggcctgccgg gacgccggct ggtggctcgc ccggtagtgg tgctcgacga
    21181 gcgccgcgtc cacccgctcc ggggcggcgt agacgcgtcc gcgcaggtgg ccctccagcg
    21241 ccgagcggct ggtcagcagg tcgagcaccg actcgcggag gacgggaagg cccagcaagg
    21301 agggcagcac cgcgccagcg tgctcaccgc cgcgctcgcc ggtaatcccg agcggggcca
    21361 ccagcgccag accgcgcacg agctcaggag cggtggcggc cacgcgcacc gcgaacgccg
    21421 ccggcaggcc ggccgccacg atcaccgcgg gctcccgcac cacccggcgg aggaactcct
    21481 cgatcacctc cggatagaac gccgggtgaa agccgcccgc cggcccgcgg gaacgccccc
    21541 agcccggcag gtcgggggcg aagacgtggt agcgcgcgcg caggagctcg gccgcggcgc
    21601 gccactcgtc ggcgtcgtgc cccggcccga gggagtggag gagcagcagc ggcgtcgcgc
    21661 ccggcgtctg gtcgagctcc tggaaagtaa tctcgccgag cttgccggag tagtggcggg
    21721 tgcgccccca gcgcacgggg tggggcggcc gcgcccggcg gcgtgccacc gcgccggcga
    21781 tcatcggccc gccgatggcc gcaccggcca gcagcagtcc ccagacgagc ttgcgtccgc
    21841 ggcgccgagg aggggtcccg gtcatggcgc ggcaggatag cacgccctca cccccgaccc
    21901 cctctcccgc ctccccgccc cgggagaggg ggaggcggac aaaacaacac ggactaacac
    21961 ggaccagcac ggacgacgga gcagggggcg gtggtgcctc cccccctggg cggtcgggcg
    22021 gtagggagtg ggggaggtcc aggagggggg ttgcttcttt tcgggcggtt gggaggcgat
    22081 ccgccccgag atctaagcct tctgaccctc gggcaagccc tctgccccgc gggtcaagcc
    22141 cttcgacccg agatccaagc cctctttccc tcgggtcaag ccgtttgccc cgagcctggg
    22201 acgggacgcc gcgtaccggt tcagagagga gcgggagccg gcgggctggg cgatccagag
    22261 ccgcgaagcc tggggcgagg tcgtcggtca tgtcgcggcc ttcgagccgg cgctgatctc
    22321 cgggctccac gtgctggacg ggctgatgcg gtcgccgctg gcgctggcgg gctttctgga
    22381 ggcctgtggc aaggtgacgc tggagcgggc gggggtgatt ctggcggaga agatggagag
    22441 ataggcaggt ggcggacggc tcggggggaa atccgggccg tccgttttca ttccaggctg
    22501 aaagaaaacc cccctcctga cctcccccgc tccctgccgc ccacccgccc agggggggag
    22561 gcaccaccgc ccgggaagag gcaagcccct cccctacatc tatatagatg tgcatggagc
    22621 cattcgcctc cccctctccc ggggaggggc cgggcaggcg ggagaggggg ccggggggtg
    22681 agggcgcgct ataatctccc cctcaaggag ccactgccat gcccgtccaa cccatcgtcc
    22741 ggctcggcca tcccgctctg cgcaccccgg cgaagccggt ggccctgccg cagatcgggc
    22801 ggccggagat ccagcagctc atcgacgaca tggtcgagac catgcgcgag gcgcccggcg
    22861 tcgggctcgc cgcgccgcag ctcggcgtcg agatgcagat cttcgtctac caggcggccg
    22921 gcaacccgga ccgcggcccg gagatcccgc tgcacgtcgt catcaacccc atgatcacgc
    22981 cccagtcccg cgagctggac tacgactggg aggggtgcct gtcgatcccc gacctgcggg
    23041 gcctggtgcc gcgccacccg caggtccggg tgcgcgggct cgaccgcgac gggcatccgc
    23101 tggactacgt cgccaccggc ttcgaggcgc gcatcgtcca gcacgagttc gaccacctga
    23161 acggcgtcat cttcctcgac cgcatgcgcg acctccactc cctctcctac accgaggagt
    23221 gggaggagta catggcacgc gaaggggagg aggacgagga gaccgccgtc ggctgattgg
    23281 cggccattga gtgggccgcg agctcgtcat cgcctgggcg ggtcgccatc accgctccaa
    23341 ctgggaagag atctgcgact cctacaggaa gcgcatcgcc cgctgggtgc cggtgcgcga
    23401 cctgccggtg cgtgcccgcg ccgccgcgga cgaccccggc cggctgcgcg ccgaggggca
    23461 ggcgctcctc gccgccctcc cccagccctg ctggatcgtc gccctggaca gccgcggcga
    23521 cagcgtcgac tccgaccaac tcgccgagcg cctgtcccgg gtcaaggccg agtggccgca
    23581 cgccatcgcc ttcgtcatcg gctccgacct gggcctcgac gccgccctcc tctcgcaggc
    23641 ccgctggacc ctgtcgctgg ggcgcctcac tcttacccat gagctggcac gggtcgtgct
    23701 atatgaacag atctttcgag cgctctcgat cgaggccgga atgagctatc atcgcgagcc
    23761 tctctgaggc cgttcaggat tatcataggg aacttttgcc gaagccctga aaggagaagg
    23821 tgagcgtggc caagacacag caggcccaga aggcccccga gccgcccggc gacagcagcc
    23881 agttcgctgc catccgtgag cgtctgttga gccagcggca ggagatcgtc gatctgtaca
    23941 agcaggacgt ccgggccggc caggagtcca cggacgacgg gaccgaggac atcgtggacc
    24001 gcgccaacaa ctcctacaac cgggagctga tgttctccct gtcggacgga gagcgggtcc
    24061 tcctcctcca gatcgaggac gccatccggc ggatcgacga gggcgcctat ggccgctgca
    24121 acaactgcgg ccagaacatc gccctgccgc gcctgcaggc ggtcccctgg acgaagttct
    24181 gcatcgactg ccaggagctc gcggagaagg ggctgctctc cgaggacgcg tcctagcgac
    24241 gcgtcctggc gccgccccgc gcccgtcctg ccgcgaggtc tccaggcctc tccgacctcc
    24301 atggctgaca tcgtcgacaa gttcctcgcc ggtctgagcc gccaggatcc cccccaggtg
    24361 gtcctggtcc agggggacct cgtcctggcg gtgcccgccg ccgagcgcat cgccgccgtt
    24421 ctggcgaaga ccgggggcgc ggtggtggag acccagcgcc actcgccctc cctgtcgccg
    24481 ctgatccagg acctccgcac cttctcccta ttcgcctcct ccaaggtggt gctggccgtc
    24541 gactccgcgg tcttcgccga ccgcgaggcg gcgagcgagc tgatcgacga cgccgcggac
    24601 gtgctgccgc tcaaggagat ggctgggcgg gcgctgtcgt ccaaggagcg ccaggcggcg
    24661 tcccgcctgt tccaggtgct gcgcctcttc gacgtcgacc cctacgccgg ggcgcccgag
    24721 cgggcgttgg gcgagctgcc ggcctgggcc ctcgaggggc gccgcagccg gggtgggaag
    24781 ccgcgcggca agaagcaggt ggaggagctg cgctccgggc tggccgtgct gctggaggcg
    24841 gcgcgcggcg aggggatcca gggggccggc gagggcgagg tcgcggagct gggggaggtg
    24901 atccgcggcg ggctccctcc cgggcatcac ctggttctcg ccgagcgggt cgtggccaag
    24961 gaccacccgc tgtccgtgct gctggaggag cgcaaggcgc tgctgcgggt gggcgaggtg
    25021 gaggccgagc gggggagctg gcagggcctc gacctgctgg ccaaggagct ggagcgccag
    25081 accggcgtcg gcatcgcccc ggacgccctg aacgagctgg cccgccgcac gctgcgccag
    25141 gaggaaggca aggggc
//
LOCUS       DASPJI010000262         6096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_646760, whole genome shotgun sequence.
ACCESSION   DASPJI010000262 DASPJI010000000
VERSION     DASPJI010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646760"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VEL74_06895"
     CDS             <1..453
                     /locus_tag="VEL74_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HYG62290.1"
                     /translation="VKRRQEDLGTGRGVTVVDDFAHHPTAVEKTLVALRQRYPGRRMV
                     ALFEPRSLTAGRTFFFDAYREAFAHADRVLFAPTFHMGRLQEEERLDFANLAGQLTDA
                     GVPAVVCADTADVLRRALEEAREGDVLVTMSSGSFDGMPHRLATALRG"
     gene            complement(457..1350)
                     /gene="ftcD"
                     /locus_tag="VEL74_06900"
     CDS             complement(457..1350)
                     /gene="ftcD"
                     /locus_tag="VEL74_06900"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HYG62291.1"
                     /translation="MPLLECVPNVSEGRRPEVVDRLISAITAGGGVRLLDVSSDPDHN
                     RTVLTLAGEPEPLHEALLALYEEALAAIDLTRHEGVHPRVGTVDVVPFIPLGDTPMEV
                     AVAAAHRLAPAVAGRFGLPVYLYERAARREERRLLAAIRRGGFEGLPAKIADPAWAPD
                     HGPARVHPTAGVTVIGARFFLIAFNAVLDTADVEVARAVARKVRESGGGLPAVRAMGV
                     YLESRGRAQVSMNLVDFRRTSILGALERVRREAEGLGARVMESEVVGLVPEEAALGVV
                     RDALQLPGFPAGKLIEDRLRR"
     gene            1447..3489
                     /locus_tag="VEL74_06905"
     CDS             1447..3489
                     /locus_tag="VEL74_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62292.1"
                     /translation="MSYPGNPSLSPDVQQRVLSTFEQTLDLAAEGNRQEALLGCDFVL
                     RMDPQFASARTLQERLRSGAGPVMVDDLRAAPAAPAFDPGFGSGSAAADPFTDFGSLS
                     LDLPDLPAESLFGTAPSPGFPPGDLRAELSALLEQRRFPELLQKAEQQMAAVAADPEL
                     QRIAATAQERLESEPYVVKFLASARDAQRSGDAKEVERLLAKARSLDPSHPGIAELER
                     SMPAAATAMPSPFGAAPAGAADSYAGGDSESARRIRELLAEGQAAFDGGDPQAAIDAW
                     SRIFLIDIDHQEASRRIEQARKLKAEKERQVEEIFHDGLAAIESRDLERARQSFQQVL
                     ALQPGHVTAREYLDQLAAGVVPVVRMTSREMPAVSAEALPTPADFDSGMADDLKEEIL
                     VPPDPSEVAAKPRARREPKPITAKANKPWRLFIGVGAAVLLLAAAGLWYLLQNRDSLF
                     PNSQPDESAMAAPASHLVDRAKNQHREGKIDLAMAQLRRVPPTDPRYKEAQDLLAQWS
                     GASPAATRPTGPSPEAMNRRQAFLDQARAAYTERSYLRAVDSFEKAASLGALEGDDAA
                     SLADAREQVAPLARFVELYNQHDWEFIVRDLWEMHLKDPGNRDVKRLLVDSYYNLAVV
                     DLQQSDAKSASNRLSEVANLAGDDETLRRQHLFAQTYQDREKDLLYRIYVKYLPFR"
     gene            3503..4180
                     /locus_tag="VEL74_06910"
     CDS             3503..4180
                     /locus_tag="VEL74_06910"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HYG62293.1"
                     /translation="MRRSDETPPEEQLLFDLPLEPPARGEAARKPAPRAPREERPEEP
                     SLPYEEPPRAPRRRSATEEARIALLQPVADPPAHEDDTEEERPRLASRLAGGGADLLL
                     HAAVGVASLVGLRFLLGVQPALSDWPAVLLFLLSFSFLYTVVPLAFWGHTLGMAWAGL
                     TSHNRDGEPLTFDQTVRRWMGGLITSGLLGLPLLLTGGGRSLADFLSGSDTYPLSGGE
                     SGEDEEK"
     gene            complement(4187..4795)
                     /locus_tag="VEL74_06915"
     CDS             complement(4187..4795)
                     /locus_tag="VEL74_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62294.1"
                     /translation="MPSEYDPLFSGSRPGDDDLEPVRERFRAAGGPYLRAPWSWFTWA
                     LLLPAVALLTPRVHRLAGPPGVLSAWSLVILLGGAVELAVILRTSKRGARGTPLASWV
                     LRVQGNLSLVALALSAVLLWQDMTWAVPGVWLLLLGHSFYTLGGIAFEPFRAAGILYQ
                     LGGVLALWPPWSDSAPLAVFAATTAIGNLWVGISVWRARRTG"
     gene            complement(4826..5224)
                     /gene="atpC"
                     /locus_tag="VEL74_06920"
     CDS             complement(4826..5224)
                     /gene="atpC"
                     /locus_tag="VEL74_06920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HYG62295.1"
                     /translation="MARNDTFHCSVITPERAVLEADATFVAFPAHDGEVGILKHRAPL
                     LYKLGTGELRVVTAEGTQRLFVDGGFAQMVEDRLTILTEQARRLDEIDRAAAQRALAE
                     ARTMPMVTDAQFAARQRAVRSAEVQIKLAS"
     gene            complement(5245..>6096)
                     /gene="atpD"
                     /locus_tag="VEL74_06925"
     CDS             complement(5245..>6096)
                     /gene="atpD"
                     /locus_tag="VEL74_06925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011122672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HYG62296.1"
                     /translation="GERTREGTDLFLEMGESGVLDKAALVFGQMDEPPGTRLRVALSA
                     LTMAEYFRDQTGVDTLLFVDNIFRFSQAGSEVSALLGRMPSAVGYQPTLGTEMGELQE
                     RITSTSRGAVTSIQAIYVPADDYTDPAPATAFAHLDSTVNLERSISEKGIYPAVDPLA
                     SSSRIIAPEYIGDRHYAVARRVQQILQRYKDLQDIIAILGVEELSEDDKVIVRRARKI
                     ERFLSQPFFVAEQFTGLAGIYCSREDTIRSFEELCDGKWDHLPDQAFMYVGAIEEAAA
                     QAERIAS"
BASE COUNT          894 a         2245 c         2135 g          822 t
ORIGIN      
        1 gtcaagcgcc gccaggagga cctcggcacc ggccgcggcg tcaccgtggt cgacgacttc
       61 gcccatcacc cgacggcggt cgagaagacg ctggtggccc tgcgccagcg ctaccccggc
      121 cgccgcatgg tggcgctctt cgagccgcgc agcctgaccg ccggccgcac cttcttcttc
      181 gacgcctacc gcgaggcctt cgcccacgcc gaccgggtgc tcttcgcccc caccttccat
      241 atgggacggc tgcaggagga ggagcggctc gacttcgcca acctcgccgg gcagctcacg
      301 gacgccggcg tgccggcggt cgtctgcgcc gacacggccg acgtgctgcg gcgggcgctg
      361 gaggaggcgc gggaggggga cgtgctggtc accatgtcct ccggctcgtt cgacggcatg
      421 ccccaccggc tggcaacggc gttgcggggt tagccctcac ctccgcagcc ggtcttcaat
      481 cagcttgccg gcggggaagc ccgggagctg gagggcatcg cggaccaccc ccagggcggc
      541 ctcctccggc accaggccga cgacctcgct ctccatcacc cgggccccca gcccctccgc
      601 ctcccgccgc acccgctcca gcgcccccag gatggaggtc cgccggaaat ccaccaggtt
      661 catgctgacc tgggcgcggc cgcggctctc cagatagacg cccatcgccc gcacggccgg
      721 cagtccgccc cccgactccc gcaccttccg ggcgacggcg cgggccacct ccacgtccgc
      781 ggtgtcgagg actgcgttga aggcgatgag gaagaagcgg gcgccgatca ccgtcacgcc
      841 ggccgtggga tggacccggg ccggcccatg gtccggcgcc caggcgggat cggcaatctt
      901 ggcgggcaac ccctcgaagc cgccgcggcg gatggccgcc agcagccgcc gctcctcacg
      961 ccgcgccgcc cgctcataga gatagacggg gaggccgaag cgcccggcca ccgccggcgc
     1021 cagccggtgg gcggcggcga ccgccacctc catgggggtg tcgccgaggg ggatgaaggg
     1081 caccacgtcg accgtcccga cccgggggtg gaccccctcg tggcgggtga ggtcgatggc
     1141 ggccagcgcc tcctcataga gggcgaggag cgcctcgtgc agcggctccg gctcgcccgc
     1201 cagggtcagg accgtacggt tgtggtcggg atcggaggag acgtccagga ggcggacgcc
     1261 cccgccggcg gttatcgcag agatcaaccg gtccaccacc tccgggcggc ggccctcgct
     1321 gacgttgggg acacactcga ggagcggcac tccgcgagag tatcgcaacg catcgtccgt
     1381 tgtgcatggg agtgcgctct gttaggatac ccgcggtttt caaccgaaag cagtgcgatc
     1441 tcgccgatga gttacccagg caacccctcg ttgtcccccg acgtccagca acgtgtgctg
     1501 agcacgttcg agcagaccct cgacctcgcc gccgagggca atcgccagga ggcgctcctg
     1561 ggctgtgact tcgtcctgcg catggacccc cagttcgcct cggcgcggac cctccaggag
     1621 cggctgcgct ccggagccgg cccggtgatg gtggacgacc tgcgcgcggc gccggccgct
     1681 ccggccttcg atcccggctt cggctcgggc tcggccgcgg cggacccctt caccgacttc
     1741 ggctcgctga gcctcgactt gcccgacctg ccggcggaga gcctgttcgg caccgctccg
     1801 agcccgggat tcccgccggg cgacctgcgc gccgagctgt cggcgctcct cgagcagcgg
     1861 cgcttccccg agctcctcca gaaggccgag cagcagatgg ccgcggtggc cgccgacccc
     1921 gagctgcagc ggatcgccgc cacggcccag gagcggctgg agtccgagcc gtacgtcgtc
     1981 aagttcctcg cctcggcgcg ggacgcgcag cgctcgggag acgccaagga ggtcgagcgc
     2041 cttctcgcca aggcgcgctc gctcgatccg tcccaccccg gcatcgccga gctggagcgg
     2101 tcgatgccgg cggcggccac ggccatgcct tcgcccttcg gcgccgcccc ggcgggcgcc
     2161 gccgacagct atgccggcgg cgactccgag agcgcccgcc gcatccgcga gctgctcgcc
     2221 gaggggcagg cggccttcga cggcggcgac ccgcaggcgg ccatcgacgc ctggtcgcgc
     2281 atcttcctga tcgacatcga ccatcaggag gcgtcgcgcc gcatcgagca ggcccgcaag
     2341 ctcaaggccg agaaggagcg ccaggtcgag gagatcttcc acgacggcct ggcggcgatc
     2401 gagagccgcg acctggagcg ggcccggcag tccttccagc aggtcctcgc gctccagccg
     2461 ggccacgtga cggcccgcga gtacctggac cagctcgccg ccggggtcgt ccccgtggtg
     2521 cggatgacgt cgcgggagat gccggcggtg tcggcggagg ccctgccgac gccggcggac
     2581 ttcgattccg gcatggccga cgacctcaag gaagagatcc tggtcccgcc ggaccccagc
     2641 gaggtggcgg ccaagccccg cgcccgccgc gagcccaagc cgatcaccgc caaggccaac
     2701 aagccgtggc gcctgttcat cggcgtcggc gccgcggtgc tgctgctcgc cgccgccgga
     2761 ctctggtacc tgctccagaa ccgcgacagc ctgttcccca actcgcagcc cgacgaatcc
     2821 gcgatggcgg ctccggcctc tcacctcgtc gaccgggcga agaaccagca ccgggagggc
     2881 aagatcgacc tcgccatggc ccagctccgc cgcgtgcccc ccaccgatcc gcgctacaag
     2941 gaggcgcagg atctgctcgc gcaatggagc ggcgccagcc cggccgccac ccggcccacc
     3001 gggccgtcgc ccgaggccat gaaccggcgc caggccttcc tcgaccaggc ccgggccgcc
     3061 tacaccgaac gcagctacct gcgtgcggtg gacagcttcg agaaggcggc gtctctggga
     3121 gcgctcgagg gggacgatgc cgcctccctc gccgacgccc gggagcaggt cgcccctctc
     3181 gcccgcttcg tcgagctcta caatcagcac gactgggagt tcatcgtgcg cgatctgtgg
     3241 gagatgcacc tcaaggatcc gggcaaccgc gacgtcaagc ggctgctcgt cgactcctac
     3301 tacaacctcg ccgtcgtgga cctgcagcag tcggacgcga agagcgcctc caacaggctc
     3361 tccgaggtgg cgaacctggc cggagacgac gagacgttgc ggcgtcagca cctcttcgcc
     3421 cagacctatc aggaccggga gaaggatctg ctctaccgga tctacgtcaa gtacctgccc
     3481 ttccggtgat gtccttcagg taatgcgccg ctcggacgag accccgccgg aagagcagct
     3541 cctcttcgac ctgccgctcg agccccccgc acgcggtgag gcggcccgca agcccgcccc
     3601 tcgggcaccg cgggaggagc ggccggagga gccctccctg ccctacgagg aaccgccccg
     3661 cgcgccgcgc cggcgctcgg cgaccgagga ggcgaggatc gccctcctcc agccggtggc
     3721 ggacccgccg gcgcatgagg acgatacgga ggaggagcgc ccccgtctgg ccagccggct
     3781 cgccggcggc ggggcggacc tgctgctgca cgccgcggtg ggcgtggcgt ccctggtagg
     3841 gctgcgcttc ctgctggggg tccagccggc cctctccgac tggccggcgg tgctgctgtt
     3901 cctgctctcc ttctccttcc tctatacggt ggtgcccctc gccttctggg gtcacaccct
     3961 cgggatggcc tgggccgggc tcacctccca caaccgggac ggcgagccgc tcaccttcga
     4021 ccagaccgtc cgccgctgga tgggcggcct gatcacctcc gggctcctgg ggctccccct
     4081 tctcctgacc ggtggcggcc gttccctcgc cgacttcctg agcggctcgg acacctaccc
     4141 actatcgggt ggagaatccg gcgaagacga agaaaagtag ggcgctctac cccgtccggc
     4201 gggcccgcca gacgctgatc cccacccaga ggttgccgat cgccgtcgtg gcggcgaaga
     4261 ccgccagcgg cgcgctgtcc gaccatgggg gccagagcgc cagcacccca cccaactggt
     4321 agagaatgcc ggcggcgcgg aacggctcga aggcgatgcc gcccagcgtg tagaaggagt
     4381 gcccgaggag caggagccag acgcccggca cggcccaggt catgtcctgc cagaggagca
     4441 cggcggacag cgccagggcc accagcgaca ggttcccctg gacgcgcagc acccaggagg
     4501 cgaggggcgt gccccgtgcc ccccgtttcg aggtgcggag aatgacggcc agctccacgg
     4561 cgccgccgag gaggatgacc agcgaccagg cggagagcac ccccggagga ccggcgagac
     4621 ggtggacgcg cggcgtgagg agggccaccg ccggcagcag cagggcccag gtgaaccagg
     4681 accagggcgc gcggagatag gggccgccgg cggcacggaa gcgctcccgc accggctcga
     4741 ggtcgtcgtc gccggggcgg gagccggaaa acagcggatc gtattcggac ggcatcggct
     4801 gcttccagcg gaccgtccgt ggagatcagc tcgccagctt gatctggacc tcggcgctgc
     4861 gcaccgcccg ctggcgggcg gcgaactgcg cgtcggtcac catgggcatg gttcgggcct
     4921 cggccagcgc ccgctgcgcc gcggcgcggt cgatctcgtc cagccgccgc gcctgctcgg
     4981 tgaggatcgt cagccggtcc tcgaccatct gggcgaagcc gccgtcgacg aacagccgct
     5041 gggtgccttc ggcggtcacc acgcgcagct cgccggtccc cagcttgtag aggagcggtg
     5101 cgcggtgctt gaggatgccg acctcaccgt cgtgcgccgg gaaggcgacg aaggtggcgt
     5161 cggcctcgag caccgcccgc tccggggtga tgacgctgca gtggaacgtg tcgtttctcg
     5221 ccatggctca agctcgcgat gcactcaact ggcaatgcgc tccgcttggg ccgccgcctc
     5281 ttcgatcgca ccaacgtaca tgaacgcctg gtccgggagg tggtcccact tgccgtcgca
     5341 cagctcctcg aaggagcgga tggtgtcctc gcgcgagcag tagatgccgg cgaggccggt
     5401 gaactgctcg gcgacgaaga agggctggga gaggaagcgc tcgatcttgc gggcgcgccg
     5461 cacgatgacc ttgtcgtcct ccgacagctc ctcgacgccg aggatggcga tgatgtcctg
     5521 caggtccttg tagcgctgca ggatctgctg cacccggcgg gccaccgcgt agtggcggtc
     5581 gccgatgtac tcgggcgcga tgatgcggct gctcgacgcg agcggatcga ccgcggggta
     5641 gatgcccttc tccgagatcg agcgctcgag gttgacggtg ctgtccaggt gggcgaacgc
     5701 cgtggccggc gccgggtcgg tgtagtcgtc ggcgggcacg tagatcgcct ggatgctggt
     5761 gacggcgccg cgcgacgttg acgtgatgcg ctcctgcagc tcgcccatct cggtgccgag
     5821 cgtcggctgg taaccgacgg cggacggcat gcggccgagc agcgccgaca cctcggagcc
     5881 cgcctgcgag aaccggaaga tgttgtcgac gaagagcagg gtatcgacgc cggtctggtc
     5941 gcggaagtac tcggccatgg tcagcgccga cagcgccacg cgcagtcgcg tgcccggggg
     6001 ctcgtccatc tggccgaaga cgagggcggc cttgtcgagc acgccggact cgcccatctc
     6061 gaggaagagg tcggtgccct cacgcgtccg ctcgcc
//
LOCUS       DASPJI010000263         6165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_588052, whole genome shotgun sequence.
ACCESSION   DASPJI010000263 DASPJI010000000
VERSION     DASPJI010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588052"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VEL74_06930"
     CDS             <1..446
                     /locus_tag="VEL74_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62297.1"
                     /translation="SGLALLLVTVVVLVSVRGRIGDALLSGLPLALGCLWTFGLWGVF
                     RLPVDLLAVSTLPVLFGTGIDLGVHAVQGGRLRPEEGIGGTLRESGLAMLLVALTTGT
                     GFGSLGASRVPGLQNAGTLVAVGVIACLAATFLVLPAVEALRRRR"
     gene            850..1209
                     /locus_tag="VEL74_06935"
     CDS             850..1209
                     /locus_tag="VEL74_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62298.1"
                     /translation="MPSSDDRTPSRVDLSRPTESTRRHLYELVVHVYFHPTPEEVEQA
                     RQDPGNHWVPDYGPDAARLHVFYAYGRWFAAWEALEEKKEAEADPSLPAWRRWSVVRI
                     VEDADPSSAMGVTFLEV"
     gene            complement(1689..3695)
                     /locus_tag="VEL74_06940"
     CDS             complement(1689..3695)
                     /locus_tag="VEL74_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYG62299.1"
                     /translation="MSIHDFLRDAVILLAASTAAVLACHKLRTPAVVGFLLTGMLIGP
                     AGLALIPEVERVTLFAEIGVVFLLFTIGLEFSLERLKEIRRFFLVGGSLQSFLTIGAA
                     AGIAIAAGMRPAPSIFLGFLLALSSTAIVLKLYADRRELDAPQGKLLIGVLLFQDFLI
                     VPMIVLTPVLAGRVEASLQSVLLRFLISLAVVAGVFVLARYLMPKLLHLIVRTQVREV
                     LVLGALLICLGMAWFTESLRFSLALGAFLAGIIISESEYSHQIVAEVVPFRDVFNSIF
                     FISIGMLLDLGFAAERAGLIAGLALLIVGVKALAAAGAGAALGLSSRVALIGGLSLAQ
                     IGEFSFVLLQVGQQNGVLPTPFFQTFIAASVLTMMATPLLVAAAPWLAERIPRLRLAG
                     LGQAASSELRDHVVIVGYGVNGRNLSRVLREASIPYRVIELNPELGRTARLSGEPLLY
                     GDATRREILEHAGIRTARVVVFAISDLIAVRRSIRLARELHPGINIVVRTRAVAEIEG
                     LQGCGANEVIAEEFETSIEIFTRVLRQYHVPSNIIRAQTRALRGEDYQMLRVPARDRQ
                     LSESLLRILAQGITDVYMLSPAHLRRAGDSIRALALRERTGATVIAVVRGQKSHTNPP
                     PDLELQDGDCLVLVGSHGEIERAFDFLDHAAAEGEARPSLAEPA"
     gene            3873..4823
                     /locus_tag="VEL74_06945"
     CDS             3873..4823
                     /locus_tag="VEL74_06945"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62300.1"
                     /translation="MSVAAPRVEIPPHAPEGVWSRRLLGPFHTTGVFWVRLHRFGVCV
                     MPDWAVRILVTLFTCFFFIVLRRVGAAIAANVDAVLGPCGWRERQARVFRTMRSFAWC
                     LSERYERLSTSRQFTLESDGWETWQELNATGEGVVLLTAHMGNWEMGSMLPSTQEARR
                     VHLVREAETDPRAQRFLSELLRSSSSELYTTHFAEDPQLGMFLLDALRHGDLVALQGD
                     RPRCGGKVEEVELFGRPFPLPVGPAALARAAGAPILPVFFFREGRLRYRCSIRQPIHV
                     ERTGDRQRDLEEAMKRFAAELQSSIAQHPHQWFCFRKVWN"
     gene            4840..5847
                     /locus_tag="VEL74_06950"
     CDS             4840..5847
                     /locus_tag="VEL74_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HYG62301.1"
                     /translation="MAGLALAIFLSFVPAFLCAAVVYWIDHFEKEPHILLGAVFGWGA
                     VVATLGAMVSQLVLSGAVHLWTGSEDTADLAGATLFAPLTEESLKGAAVLLVFLVLRR
                     EFDSLLDGIVYAGIVALGFAATENVLYLYGAFLEGGMEDLLSIAFLRVGLGAWDHPFY
                     TAFIGIGLAAARLSRSAAVRWLAPPAGWGMAVFTHSLHNSLATASEHMPALGLLMFLI
                     DWSGWLAISLLIFWSLHQEGRLLALHLADEVDRCLTPQQYRTASSLGTQIGARFRALG
                     SGRLGSTRRFYRLCGELAHKKHQLALLGDEGGNSLAIEGLRAELARLSGQADCDRSSG
                     A"
     gene            5884..>6165
                     /gene="msrA"
                     /locus_tag="VEL74_06955"
     CDS             5884..>6165
                     /gene="msrA"
                     /locus_tag="VEL74_06955"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HYG62302.1"
                     /translation="MEKATFGAGCFWGVEAAFRRIKGVKSTAVGYAGGWKENPTYQEV
                     CTDGTGHAEVVQVEYDPSEVSYEDLLEVFWSNHNPTTLNRQGPDIGTQYR"
BASE COUNT          930 a         2124 c         2174 g          937 t
ORIGIN      
        1 cgagcggcct cgccctgctg ctcgtcaccg tcgtcgtgct cgtctccgtc cgcggccgga
       61 tcggcgacgc gctcctctcc ggcctccccc tggcgctggg ctgcctgtgg accttcggcc
      121 tctggggggt gttccggcta ccggtcgacc tcctggcggt ctccaccctg ccggtcctct
      181 tcggcaccgg catcgacctc ggggtgcacg cggtgcaggg gggacggctg cggccggagg
      241 aggggatcgg cggcaccctg cgggagtcgg ggctcgccat gttgctggtg gcgctcacca
      301 ccggcaccgg cttcggcagc ctcggcgcct cgcgggtgcc ggggctccag aacgccggca
      361 ccctggtcgc cgtaggggtg atcgcctgcc tggcggcgac cttcctggtg ctgccggcgg
      421 tggaggcgtt gcggcgacgc cggtgacccg caacacgggc ggacacggat taccacggac
      481 gatcacggat cccggagcag ggggcggtgg tgcctccccc cctgggcggg tgggcggtag
      541 ggagcggggg aggtccagga ggggggtttt tctccgcccc cgcgatcttc aacctccccg
      601 caagaatcct ccatttccct cttgacgcgc cgccaatccc gggtatagcc ttcgaggcgg
      661 acgtaagccg tacaagaagg aggtggtagg gggaaagaag gaaaccgcgg tttcacacca
      721 cagccgtaca ggaccgctgt gcctccgagt tgagcgcgta gctcatccgg agccccgggg
      781 tcccagacgg ccaagggagg gagcggcaaa ctcacctccc gaggcctagc cggacaggag
      841 aacacactca tgcccagctc agatgatcgt acgccttcgc gcgtcgatct gtcccgtccc
      901 accgaatcca cccgccgtca tctctacgag ctggtggtcc atgtctactt ccatcccacg
      961 cctgaagagg tagagcaggc ccgtcaggac cccggcaacc actgggtgcc ggactacggt
     1021 cccgacgcgg cgcgcctgca cgtcttttac gcctacggcc gctggttcgc tgcctgggag
     1081 gcgctggagg agaagaagga ggcggaggcc gatccgtcgc ttcccgcgtg gcggcgctgg
     1141 tcggtggtgc ggatcgtcga ggacgcggac ccgtcgagcg cgatgggcgt cacctttctc
     1201 gaagtctgac gccgcccggg cggtgggtcc cgcttcccgg ggcccgccgc cgtttccccc
     1261 atggaaaagg aacggccccg tcgccgcgga tgcgggacgg ggctcctgga agcgatccgg
     1321 acgccgcgga tgccatccag acgctgtggg cttcatccag acgcctcgga tgccatccag
     1381 acgcttcgga agtttcccga gcgcttcgga cgcgatccaa ttcaaaagga tggagtccgc
     1441 aggacccgga aagcttcccg acggctcgga ctgcgcccaa ttcaaaagga tggattccga
     1501 ggcagcgggg agcttcccgg acgcttcgga tcgctggcgc gaagaaaaac ccccctcctt
     1561 gacctccccc gctccctccc acccgaccat ccaggggggg aggcaccacc accccctcga
     1621 cgctcaacgt tccctgtccg tctccccctc tcccggggag gcgggagagg gggccagggg
     1681 gtgagggcct acgccggctc ggccagcgac ggccgggcct ccccctccgc cgccgcatgg
     1741 tccaggaagt cgaaggcgcg ctcgatctcg ccgtggctcc ccacgagcac caggcagtcg
     1801 ccgtcctgca gctccaggtc cgggggcggg ttggtgtggg atttctggcc gcgcacgacc
     1861 gcgatcaccg tcgccccggt ccgctcccgc agggcgaggg cgcggatgga gtccccggcg
     1921 cgccgcaggt gggcggggga gagcatgtag acgtcggtga tcccctgggc gaggatgcgc
     1981 agcagggatt cggagagctg gcggtcgcgc gccggcacgc gcagcatctg gtagtcctcg
     2041 ccgcgcagcg cccgcgtctg cgcccggatg atgttgctcg gtacgtggta ctgccgcagg
     2101 acgcgggtga agatctcgat cgaggtctcg aactcctcgg cgatcacctc gttggcgccg
     2161 cagccctgga gcccctcgat ctccgccacg gcgcgcgtgc ggacgacgat gttgatgccc
     2221 gggtgcagct cgcgggcgag acggatgctc cgccgcaccg cgattaggtc ggagatggcg
     2281 aagaccacca cccgcgcggt gcggatgccg gcgtgctcca ggatctcccg gcgggtggcg
     2341 tcgccgtaga gcagcggctc gcccgacagg cgcgccgtcc gcccgagctc cgggttcagc
     2401 tcgatcaccc ggtaggggat cgacgcctcg cgcagcaccc gcgacaggtt ccggccgttg
     2461 acgccgtagc cgacgatgac gacgtggtcg cgcagctcgg aggacgcggc ctggccgagc
     2521 ccggccaagc ggagccgcgg gatccgctct gccagccagg gcgccgccgc gaccaggaga
     2581 ggcgtcgcca tcatggtgag caccgaggcg gcgatgaagg tctggaagaa gggggtgggg
     2641 agcaccccgt tctgctggcc gacctggagg agcacgaagg agaactcgcc gatctgggcg
     2701 aggctcaggc cgccgatcag ggcgacccgc gagctgagcc cgagggcggc gccggcgccc
     2761 gcggcggcga gcgccttcac cccgacgatg agcagggcga gaccggcgat cagcccggcg
     2821 cgctcggcgg cgaagccgag gtccagcagc atgccgatcg aaatgaagaa gatgctgttg
     2881 aagacgtcgc ggaacggcac cacctcggcg acgatctggt ggctgtactc cgattcggag
     2941 atgatgatgc cggccaggaa ggcgccgagg gcgagcgaga agcgcaggct ctcggtgaac
     3001 caggccatcc ccaggcagat cagcaacgcg ccgagcacca ggacctcgcg cacctgggtg
     3061 cggacgatga ggtggaggag cttcggcatc aggtagcgcg ccaggacgaa gacgccggcg
     3121 accaccgcca gggagatgag gaagcgcagc agcaccgact ggagcgacgc ctcgacccgc
     3181 ccggcgagca ccggcgtcag cacgatcatc ggtacgatga ggaagtcctg gaagaggagg
     3241 acgccgatga ggagcttgcc ctgcggcgcg tcgagctcgc gccggtcggc atagagcttt
     3301 agcacgatcg ccgtgctgct cagcgccagc aggaagccga ggaagatcga cggcgccggc
     3361 cgcatcccgg ccgcgatggc gatgccggcg gcggcgccga tggtgaggaa cgactgcagc
     3421 gagccgccga ccaggaagaa gcggcggatc tctttcaagc gctcgagcga gaactcgagg
     3481 ccgatggtga agagcaggaa gacgacgccg atctcggcga acagcgtcac ccgctctacc
     3541 tccgggatca gggcgagccc ggccggcccg atcagcatgc cggtgagcag gaagccgacc
     3601 acggccggag tccgcagctt gtggcaggcg agcaccgccg ccgtcgaggc ggcgaggagg
     3661 atgacggcgt cgcgtagaaa gtcgtggatc gacaagcccg gtgcgctctc cctgagggtc
     3721 aggagggtca ggaaccgtta gaggaggagt atacccgcgg gaatcctacc cgctggggtc
     3781 ccggttgtgg cggttctgga ggcttccggc cgcggcgggg tataatccag gaagcccaag
     3841 ctcgggagta atatcggagg tcgtcgcagg atatgagtgt cgccgcgcca cgtgtggaga
     3901 ttccgcccca tgctccggaa ggggtctggt cacgccgtct tctcgggccc ttccacacca
     3961 cgggggtctt ctgggtccgc ctgcaccggt tcggggtgtg cgtcatgccg gactgggcgg
     4021 tgcggatcct cgtcacgctg tttacctgct tcttctttat cgtcctgcgc cgggtcgggg
     4081 cggcgatcgc ggccaacgtc gacgccgtcc tggggccctg cggctggcgg gagcgccagg
     4141 cgcgcgtctt ccgcaccatg cgctccttcg cctggtgcct gtcggagcgg tacgagcgcc
     4201 tgagcacgtc ccggcagttc acgctcgaat ccgacgggtg ggagacctgg caggagctga
     4261 acgcgaccgg cgagggtgtg gtgctgctca ccgcgcacat gggcaactgg gagatgggct
     4321 cgatgctccc ctccacccag gaggcgcggc gggtccatct ggtgcgcgag gccgagaccg
     4381 acccccgcgc ccagcggttc ctgtcggagc tgctgcgcag cagctcctcc gagctctaca
     4441 ccacccactt cgccgaggac ccgcagctcg gcatgttcct gctcgacgcc ctgcgccacg
     4501 gcgacctcgt cgccctgcag ggggaccggc cgcgttgcgg cggcaaggtg gaggaggtgg
     4561 agctgttcgg ccgcccattc ccgctgcccg tcggcccggc cgccctcgcc cgtgccgccg
     4621 gcgcgccgat cctgccggtc ttcttcttcc gcgaggggcg cctgcgctac cgctgctcga
     4681 tccgtcagcc gatccatgtc gagcgcaccg gcgaccgcca gcgcgacctg gaggaggcga
     4741 tgaagcgctt cgcggcggag ctccagtcct ccatcgccca gcacccccac cagtggttct
     4801 gcttccgcaa ggtctggaac taggcgaaca tcgttcgcca tggcgggcct cgccctcgcg
     4861 atcttcctga gcttcgtccc ggcgttcctc tgcgccgccg tcgtctactg gatcgaccac
     4921 ttcgagaaag agccccacat cctcctcggg gcggtcttcg gctggggggc cgtggtggcc
     4981 accctcggcg ccatggtctc gcagctcgtc ctgtccggtg ccgttcacct ctggaccggc
     5041 tcggaggaca ccgccgacct cgccggggcg acgctgttcg cgccgctcac cgaggagagc
     5101 ctcaaggggg cggcggtgct gctggtcttc ctggtgctgc gccgcgagtt cgattcgctc
     5161 ctggacggca tcgtctatgc cggcatcgtg gccctgggct tcgcggcgac ggagaacgtc
     5221 ctctatctct atggcgcctt cctggagggg gggatggagg acctgctgtc catcgccttc
     5281 ctccgcgtcg gcctcggcgc ctgggatcac ccgttctaca ccgccttcat cggcatcggc
     5341 ctcgccgccg cccggctgag ccgctcggcc gcggtgcgct ggctggcacc gcccgcgggc
     5401 tggggcatgg ccgtcttcac ccactcgctg cacaacagcc tggccaccgc ctccgagcac
     5461 atgccggcgc tcggcctgct gatgttcctc atcgactggt cgggctggct cgccatcagc
     5521 ctcctcatct tctggtccct ccaccaggag gggcgcctcc tggcgctcca tctggcggac
     5581 gaggtggacc gctgcctcac cccgcagcag taccgcaccg cctcctcttt ggggacgcag
     5641 atcggcgccc gcttccgcgc cctgggctcg ggccggctcg gctccacccg ccgcttctac
     5701 cggctatgcg gcgagctggc gcacaagaag caccagctcg ccctgctggg ggacgagggg
     5761 ggcaacagcc tggccatcga gggcttgcgg gcggagctgg cgcgcctgtc ggggcaggcc
     5821 gactgcgata gatccagtgg ggcttgacgg cccggatata ctggcggacg aaggagagac
     5881 accatggaaa aagccacatt cggagccggt tgcttctggg gagtcgaagc cgcgttccgt
     5941 cgcatcaagg gtgtgaagtc gaccgctgtg ggctacgccg gcggctggaa ggagaatccc
     6001 acgtatcagg aggtctgcac cgacggcacc ggtcacgccg aggtcgtgca ggtggagtac
     6061 gacccgtcgg aggtctccta tgaggacctc ctggaggtct tctggtcgaa ccacaacccg
     6121 acgaccctga accgccaggg cccggacatc ggcacgcagt accgc
//
LOCUS       DASPJI010000264        11734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_702147, whole genome shotgun sequence.
ACCESSION   DASPJI010000264 DASPJI010000000
VERSION     DASPJI010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11734)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11734
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702147"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>11734)
                     /locus_tag="VEL74_06960"
     CDS             complement(<1..>11734)
                     /locus_tag="VEL74_06960"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62303.1"
                     /translation="WSAAARVRAGETGPVSIGRPIAATRIHLLDGHLQPVPLGATGEL
                     WIGGAGLARGYLGRPDLTADRFLPNPLAAEPGERLYRTGDLARHLTGGRMEVLGRIDH
                     QVKVRGFRIELGEIETVLAALPGVRQAVVVASNSPSGDRRLVAYVVGTEEKEALLQGL
                     RERLPEYMVPSALVMLPALPLTPNGKVDRKALLDGGAAPEWGRAEESFVAPRTAMEEI
                     LAGIWAEVLGLERAGATDHFFELGGHSLLATRVISRLRGTLGVEVPLRDLFAAPRLAD
                     FAGRVEAARGTAASAPPLLAVPREGDLPLSFAQQRLWFIDQLEPGSPLYNMPGALRAE
                     GPLDGAVLARCLEEIVRRHEALRTVFAAPEGSPVQVIQPAGPFLLPVVDLSALPDSER
                     ETLAVSLTGEEAARPFDLTGGPLLRGSLLRLGEMDHVVLLTLHHIVSDGWSMGILVRE
                     IAALYPALAEGRPSPLPELPVQYADYAVWQRSWLRGEVLENEIDFWRQQLAGLPALLE
                     LPTDRPRPAVLSHRGATRPVRLPAALTRQVEALGRREGVTLFMALLAAFQTLLARYSG
                     QDDLAVGTPVAGRNRVETEGLIGLFVNTLVLRGDLTGNLPGGRAGAPTFRELLGRVRE
                     TALAAYLHQDVPFEKLVDELAPERSLAQTPLFQVMFELQNVPIGNLDLQGVSLRPAGD
                     TVATAKFDLTLALEERDGGLGGTMEHAADLFDAATIDRLTGHFERLLAAAAAEPDKPV
                     FDLPLASEAESAQILREWNDTRAEVFAKESCLHLEVAAQAARTPSAVAVEMGAERWTY
                     RRLIGSARRLALHLRGLGVGPDSIVGLYTERSPAMAVGMLAILEAGGAWLPLDPAYPA
                     ERLAFLIDDADARVLLAQESLRDRVPAEGLSLVLLDEARWDAGEDMGQPLHSGVTAGN
                     LAYVIYTSGSTGQPKGVMLTHRGICNRLRWAKQAYGIDEQDAFLQKASLGFDVSVWEC
                     FAPLISGARLVLADPGSQGDATYLARAIREHQITFIDFVPSMLSAFLAEEGVEQCVSL
                     RQIIAGGETLTPELRDRALARLPVPLDNTYGPTEVTIDTTRWVCEPGQEPHRVPIGRP
                     IANSRLYVVDAELRPVPVGVTGELLVGGTGVSRGYLRRPGLTAERFVPDPFGGRPGDR
                     LYRTGDLVCWLPDGSLDFLGRLDHQVKVRGFRIELGEIEAALAALPGVREAVVVLRES
                     RLVAYVAGDFAVEELRRSLGERLPDYMVPSAFVKLAALPLTANDKVDRKALPAPEPAG
                     ACEGYVAPRTREEEILAAVWAQVLRLPRVGVNDNFFELGGDSILSVQIVARARQAGLS
                     FTVRQIFEHQTVAGLARHVTHTDATDGLGAEQGAVTGEVPLTPIQRWFFEQDFADPHH
                     FNQALLLEAREPLAPAALDRAMAVIVEHHDALRMRFDGERQENAPAEPAATTAPFHQV
                     DLSGLPATSRDEAFERAAAALQAGFDLASGPLTRLCLFEAGGQPSRLLWVTHHLVVDG
                     ISWRVLLEDLESAYRQVTLPPKTTSFQEWARRLNGHAETLASELEHWRETIRVAVPRL
                     PVDFPSDEAAAGNLIGDEATVSFELSAEETTDLLQTVPSVYHSRIDDALLSALVRALA
                     GWTGSPCMRVDLEGHGREPLFDGVDDLDVSRTVGWFTSVYPVVLEAGDAGPGEALVSA
                     KEQLRAVPGRGIGYGLLQAAGLLEWAPAAEISFNYLGQADATAGELSLFRASTAATGP
                     VQSPRAHRTHALEIGGIVTDGRLRITLTYGSRTHLRETVERLAAGYAGALRQLIQHGP
                     DSEEVFTPSDFPKARLGAQGFQRLVSLLSDLKDVEDIYPLTPLQSGMLFHSLMSPESG
                     VYVTQVTCALPANLDRGLFRQAWERLVERHGVLRTAFLWEGLYESRQVVRKRISLPWQ
                     ELDWSGLSAEQREPRLEELRLRERHTPLPLDSAPLMRFALIRLGGEIRFIWTSHHLLL
                     DGWSLPLLVQELAAVYAALREGREPALPPARPFSDYVVWQQKQDAAKAEPFWRGELAG
                     FSTPNSLGIVGAEHPAERAAGYAEHGIRLSREVTAELQALAARHKLTLQTVTLGAWAV
                     LVSRYSGDEDVVFGGVVSGRPAALPGVETMVGMFVNTLPVRVRVDGAGLLAPWLRRLQ
                     EHQLARQDFEHTPLAQIQKWSDVPAGSPLFETLYVFENYPDIDKGGDGGGLRLRDLRS
                     FESTNYPVTLTLTAADQVSLHLATDRARVDGDAVPRLLQHLATLLTGMAEAERPIESL
                     PLLTGAERQQLLEWGAVRQELAAASTLHERFATRARLAPDAAALTCGDVTLSYGELHR
                     RSNQLAHWLRRQGVGLESRVGLRLDRSVDLVVGILGVLEAGAAYVPIDPDSPRERLAF
                     VLEDAGIQTVVGAEELALADTLPAGDLEPMADASSLAYVIYTSGSTGRPKGTLIPHGN
                     VTRLFDGTAAWFGFGERDVWTLFHSYTFDFSVWEIWGALLYGGRLVIVPWEVSRSPEL
                     FLDLLDSERVTVLNQTPSAFAQLARVDEQRHTAETSLRLVIFGGEALDPASLEPWFER
                     HGDTQPRLVNMYGITETTVHVTYRPLSALSAASARGEHRSLIGVPIPDLSLAVMDRSL
                     RPVPIGVPGELVVGGAGLARGYLGRPELTAERFVPDPSGERPGERLYRSGDLGRYLPS
                     GDVEYLGRLDHQVKIRGFRIEIGEIEAALSALAGVRQAVVLVREDRPGDRRLVAYVVG
                     GMSAAELRQSLRERLPDYMVPAAFVTLAALPLTTNGKVDRKALPTPEQSGASEGYVAP
                     RTREEEILAMVWTQVLRLNQVGVNDNFFELGGDSILSVQIVARARQAGLLFGVRQIFE
                     HQTVAELARHAMAMDAGGAFRTEQGPVTGEVPLTPVQSWFFEQGFADPHHYNQALRLE
                     PRELLSPAALERAMAVIVEHHDALRMRFDGTRQENAESEPLTPFHQVDLSGLPASRRG
                     EAYESAATDLQAGFDLFNGPLTRLCLFGAGDQPSRLLWATHHLVVDGVSWRVLLEDLE
                     AAYRQAALPPKTTSFQEWARRLTGHAEALAGELDYWRDTVQVPVPRLPVDFPEAGNLN
                     EDEATVTFELTAEETTDLLQTLPVVYHSRIDDALLSALVRALRDWTGSPRLRVDLEGH
                     GREPLFAGVDDLDVSRTVGWFTSIYPVVLEAGDAGPGDALVSAKERLRAVPGRGIGYG
                     LLRSAGLLEEAPAAEVLFNYLGQADATAEALSLFHASTTTTGPNRSPRAHRTHLLEVS
                     GMVDGGRMQISLTYGSRAHRRETMERLAEAYAGALRQLLRHARESEEVFTPSDFPKAA
                     LDSHGFQQLTALLPDLREVEDIYPLTPLQSGMLFHSLMSPASGVYVTQVTCTLPADLD
                     SRLFLQAWERLVERHAVLRTAFLWEGLDEPRQVVRKRCALAWQELDWRGLAAEERELR
                     YEELRHDDRHAPIPLDRAPLMRFSLIRVDGELRFIWTSHHLLLDGWSLPLLVQELGAV
                     YPALREGREPLLPPARPFSDYAVWLQKQDLSLVEPFWREELAGFSAPNSLGILGVDHP
                     VETAAGYDEHEILLSREVTAELQALAARHKLTLQTVTLGAWAALVSRYSGSEDVVFGG
                     VVSGRPAALPGVETMVSMFVNTLPVRVRVDGAELLAPWLQRLQERQLARQELEHTPLA
                     QIQRWSEVPAGSPLFETLYVFENYPDADGAGSGGLGIRDLRSFESTNYPVTLVLTAAD
                     QVSLHLATDRARVDRDAAPRLLRHLATLLACMAAGMDERSARRTGELDLLTPAERRQI
                     LGEWNDTQVEGIAEGCLHHAVALQAARTPSVVAVELGSEQWTYRRLVGSARLLARHLR
                     ELGVGPDEVVGLCADRSPIMVVGMLAILEAGAAYLPLDPAYPQDRLAFMVDDSGARVL
                     LLQEHLRERLGAAADGLNTVPLDARWNSDEETEALGVDV"
BASE COUNT         1897 a         4160 c         4074 g         1603 t
ORIGIN      
        1 cacgtcgacg ccgagagcct ctgtctcctc gtcagaattc cagcgtgcgt ccagaggtac
       61 ggtgttgagg ccgtccgccg ccgctcccag ccgctcgcga aggtgctcct ggagcaacag
      121 gacccgggcg cccgagtcgt cgaccatgaa ggcgagccgg tcctgcgggt aggccggatc
      181 gagaggcagg taggcggccc ccgcttccag gatggcgagc atgcctacga ccatgatcgg
      241 cgagcggtcg gcgcagagac cgacgacctc atcggggccg acgccgagct cgcgcaggtg
      301 acgcgccagg agccgcgcgc tgccgaccag acggcggtat gtccactgtt cgctccccag
      361 ctcaacggcc acgacggacg gcgtccgggc cgcctggagg gcgacggcat gatgaaggca
      421 gccctccgcg atcccctcga cctgcgtgtc gttccactcg cccaggatct gccgccgctc
      481 ggccggggtg agcagatcca gctcgccggt gcggcgcgcc gatctttcat ccatgccggc
      541 ggccatacag gcgagcagcg tggcgaggtg ccggagcagg cgcggcgccg cgtcccggtc
      601 cacgcgggcc cggtcggtcg ccaggtgcag ggagacctgg tctgccgccg tgaggaccag
      661 ggtgacgggg tagttggtgc tctcgaagct gcgcaggtcg cggatcccca ggccaccgga
      721 gccggcgcca tcggcgtcgg ggtagttctc gaagacatag agggtctcga acagcggcga
      781 gccggcgggc acctcgctcc agcgctggat ctgggccaga ggggtgtgct cgagctcctg
      841 acgcgcgagc tggcgctcct ggaggcgttg cagccaggga gccagaagct cggcgccgtc
      901 gacccgcacc cgcaccggca gggtgttgac gaacatgctg accatggtct cgactccggg
      961 cagcgccgcc ggacggccgg agaccacgcc gccgaagacc acgtcctcgc tgccgctgta
     1021 gcggctgacc agcgcggccc aggcaccgag ggtgacggtc tgcagggtca gcttgtgccg
     1081 cgccgccagc gcctgcagct ccgccgtgac ctcgcgggag aggaggatct cgtgctcgtc
     1141 ataacccgcg gctgtttcca ccgggtggtc cacgccgagg ataccgaggg aattgggggc
     1201 gctgaacccg gcgagctcct cacgccagaa cggctccacc agcgacaggt cctgcttctg
     1261 cagccagacg gcgtagtcgc tgaacggtcg cgccggcggc agcaggggct cgcggccttc
     1321 ccgcagggcc gggtagacgg ctcccagctc ctgcaccagt agtggaagcg accaaccgtc
     1381 cagcagcagg tggtgcgacg tccagatgaa ccggagctcg ccgtcgacac ggatcaggga
     1441 gaagcgcatc agcggcgcgc ggtccagggg gatcggagcg tgccggtcgt cgtgccgcag
     1501 ctcctcgtag cgcagctccc gctcctccgc ggcgaggccg cgccaatcca gctcctgcca
     1561 ggccagagcg cagcgcttgc ggacgacctg ccggggctcg tccagaccct cccagaggaa
     1621 ggcggtgcgc agcaccgcgt gacgctcgac gagcctctcc caggcctgca ggaacagcct
     1681 ggagtcgaga tccgccggca gggtgcaggt gacctgggtg acatagaccc cggacgcggg
     1741 agacatcagg ctgtggaaca gcatgccgct ctggagtggc gtgaggggat agatgtcctc
     1801 cacctcccga agatccggga gcagcgccgt cagttgctgg aagccatgcg agtcgagcgc
     1861 cgccttgggg aagtcggaag gcgtgaacac ctcctcgctc tcccgggcgt gccggagaag
     1921 ctgccgcagc gcgccggcgt aggcctcggc cagacgctcc atggtctccc ggcgatgagc
     1981 ccgcgagccg taggtcaagg agatctgcat ccggccgccg tccaccatac cgctgacctc
     2041 cagcaagtgg gtgcgatggg cgcgcgggct ccggttgggg ccggtggtgg tcgtggaggc
     2101 atggaagagc gagagcgcct cggcagtagc gtcggcttgg cccagatagt tgaacaggac
     2161 ctcggccgcc ggcgcctctt ccaggagacc ggccgaccgc agcaggccgt agccgatgcc
     2221 gcgaccgggc acggcgcgca gccgctcctt ggcactcacc agagcgtcgc cagggccggc
     2281 gtcgcccgcc tccagcacga ccggatagat cgaggtgaac cagcccacgg tgcgcgacac
     2341 gtcgaggtcg tcgacgccgg cgaacagcgg ctcgcggccg tgaccctcca ggtctacccg
     2401 caggcgcggc gagcccgtcc aatccctcag tgcccggacg agggcgctga gcagcgcgtc
     2461 gtcgatgcgg ctgtgataga ccaccggcag ggtctggagc agatccgtgg tctcttcggc
     2521 ggtgagctcg aaggtgaccg tggcctcgtc ctcgttgagg ttgccggcct ccggaaagtc
     2581 caccggcagg cggggcaccg ggacctggac ggtgtcgcgc caatagtcca gctcgccggc
     2641 caacgcctcg gcgtgaccgg tcaggcggcg cgcccactcc tggaacgagg tggtcttggg
     2701 cggcagggca gcctggcgat acgccgcctc caggtcttcc agcagcacgc gccaggagac
     2761 tccgtccacc accagatgat gggtagccca gagcagccgc gacggctggt ccccggcccc
     2821 gaacaggcag agacgagtga gtggtccgtt aaacaggtcg aagccggcct ggagatccgt
     2881 ggccgccgac tcgtaggctt caccccggcg agatgccggc aggcccgaaa ggtcgacctg
     2941 gtggaagggt gtcagcggct cggattcggc gttctcctgc cgcgtaccgt cgaatcgcat
     3001 gcgcagagca tcgtggtgct ccacgatcac ggccatggcg cgctccagag cggcgggaga
     3061 gagaagctcc cgtggctcca gccggagggc ctggttgtag tggtgcgggt ccgcgaaacc
     3121 ctgctcgaag aaccagctct ggacgggagt gagaggcacc tcgcccgtca ccggtccctg
     3181 ctcggtacgg aaggcaccac ccgcatccat ggccatggcg tggcgggcga gctcggccac
     3241 ggtctggtgc tcgaagatct gccgtacacc gaagagcagg ccggcctgac gcgcccgcgc
     3301 cacgatctgg acggagagga tcgaatcgcc gcccagctcg aagaagttgt cgttcactcc
     3361 cacctggttc aggcgcagca cctgcgtcca gaccatcgcg aggatctcct cctcgcgggt
     3421 gcgcggcgcc acataacctt cgctcgcgcc agactgctca ggcgtcggca gggctttcct
     3481 gtccaccttg ccgttggtcg tgagaggcag cgccgcgagc gtcacgaagg cggccggcac
     3541 catgtagtcc ggcagccgct cacgcagcga ctggcgcagc tcggcggccg acatgccgcc
     3601 gaccacatag gcgaccagac gccggtcacc aggcctgtcc tcgcgcacca gaacgaccgc
     3661 ttggcggact ccggccagcg ccgacaacgc ggcctcgatc tctccgatct cgatacggaa
     3721 gccgcggatc ttcacctggt gatcgagccg tccgaggtac tccacgtcgc cgctgggtag
     3781 atagcgcccc aggtcgcccg accggtagag ccgctctccc ggccgttcgc cggaggggtc
     3841 cggaacgaag cgttcggcgg tcagctcggg ccggcccagg tagccgcggg ccagccctgc
     3901 cccacccacc accagctcac ccggtacgcc gatgggaacc ggcctcaggc tccggtccat
     3961 gacagccagc gacaggtcgg ggatgggcac gccgatgagg ctgcggtgct cgccccgggc
     4021 gctggctgca gacagcgcgg atagcggccg gtaggtgaca tgcaccgtcg tctcggtgat
     4081 gccgtacatg ttgaccagcc gcggctgcgt gtcgccatgg cgctcgaacc acggctccag
     4141 gctggccggg tcgagcgcct cgccaccgaa gatcaccaac ctcagcgacg tctctgctgt
     4201 atggcgctgc tcgtccaccc gcgcgagctg ggcgaacgcc gaaggggtct ggttgagcac
     4261 cgtgacccgc tcgctgtcca gaaggtccag gaacagctcg ggcgagcggc tcacctccca
     4321 gggcacgatc accagccggc cgccgtagag cagggctccc cagatctccc agaccgagaa
     4381 gtcgaaggtg taggagtgga acagcgtcca cacgtcgcgc tcgccgaaac cgaaccatgc
     4441 cgcggtgccg tcgaacagcc gtgtgacgtt gccgtggggg atcagagtcc ccttgggccg
     4501 gccggtggaa cccgaggtgt agatcacgta agccaggctc gacgcatccg ccatgggctc
     4561 gagatcaccg gcagggagcg tgtccgccag cgcgagctcc tcggccccca ccacggtctg
     4621 gatacccgcg tcctccagca cgaaggccag ccgctcgcgc ggggaatcgg gatcgatcgg
     4681 cacataggcg gctccggcct cgagtacgcc caggattccc accaccaggt cgacggagcg
     4741 gtccaggcgc agaccgacgc ggctctcgag gcccacgccc tgccgacgca accagtgggc
     4801 cagttgattg gaccgccggt gcagctcgcc gtaactcagg gtcacgtcac cacaggtgag
     4861 cgccgcggca tcgggagcca gccgcgcgcg ggtcgcgaaa cgctcatgga gcgtggaggc
     4921 cgcggcgagc tcctgccgga ccgctcccca ctccaggagc tgctgccgct ccgccccggt
     4981 gagcagcggc agcgactcga tcggccgctc cgcttcggcc ataccggtga gcagcgtggc
     5041 gaggtgctgg agcagacgtg gcaccgcatc cccgtccacg cgggcgcggt cggtcgcgag
     5101 gtgcagggag acttggtccg ccgccgtgag ggtcagggtg acgggatagt tggtgctctc
     5161 gaagctgcgc aggtcacgca gccgcaggcc accgccgtct ccccccttgt cgatatcggg
     5221 gtagttctcg aatacgtaga gcgtctcgaa cagcggcgag ccggcgggca cgtcgctcca
     5281 tttctggatc tgggccagcg gggtgtgctc gaagtcctgt cgcgcgagct ggtgctcctg
     5341 caggcgtcgc agccagggag ccaacagccc ggccccatcc acccgcaccc gcaccggcag
     5401 ggtgttgacg aacatgccga ccatggtctc gacgccgggc agagcggccg gacggccgga
     5461 gaccacaccg ccgaagacca cgtcctcgtc gccgctgtag cggctgacca acacggccca
     5521 ggcgccgagg gtgacggtct gcagggtcag cttgtgccgc gccgccagcg cctgcagctc
     5581 tgccgtcacc tcgcgggaga gccggattcc atgctcggcg tagcccgcgg ctctctccgc
     5641 cgggtgctcc gcaccgacga tgccgagaga gttgggggtc gagaatcccg cgagctcccc
     5701 gcgccagaac ggctccgcct tcgccgcatc ctgcttctgc tgccagacga cgtagtcgct
     5761 gaacggccgc gccggcggca gagccggctc ccgtccctcc cgcagggcag cgtagacggc
     5821 cgcgagctcc tgcaccagga gagggagcga ccagccgtcc agcagcaggt ggtgggacgt
     5881 ccagatgaac cggatctcac cgccgagacg gatcagggcg aagcgcatca gcggcgcgct
     5941 gtccagaggc agaggcgtgt gccgctcacg aagccgcagc tcttcgaggc gcggctcccg
     6001 ctgctccgcg gagaggccgc tccagtccag ctcctgccag ggtagggaga tccgcttgcg
     6061 gacgacctgc cgggactcgt agaggccttc ccagagaaag gccgtgcgca gcactccgtg
     6121 acgctcgacg agcctctccc aggcctgccg gaacagcccc cggtcgaggt tcgccggcag
     6181 ggcgcaggtg acctgggtga catacacccc ggactcgggc gacatcaggc tgtggaacag
     6241 catgccgctc tggagcggcg tgagggggta gatgtcctcg acgtccttca gatccgatag
     6301 aagcgatacc agcctctgga agccctgcgc gccgagcctg gccttgggga agtcggacgg
     6361 cgtgaagacc tcttcgctat ccgggccgtg ctggatcagc tgccgcagcg cgcccgcata
     6421 gcccgcggcc agccgctcca cggtttcccg gagatgcgtc cgtgagccgt aggtcaaagt
     6481 gatccggagc cggccatcgg tcacgatgcc accgatctcc agcgcgtgag tgcggtgggc
     6541 gcgcgggctc tgcaccgggc cggtggcggc cgtggacgcc cggaaaagtg acagctcccc
     6601 ggcggtggcg tcggcctggc ccagatagtt gaagctgatc tcggccgccg gagcccattc
     6661 caggaggccg gccgcctgca acaggccata gccgatgccg cgcccgggca cggcgcgcaa
     6721 ctgctccttg gcgctcacca gggcctcgcc gggaccggcg tcgcccgcct cgagaaccac
     6781 cgggtagacg gaggtgaacc agcccacggt gcgcgagacg tcgaggtcgt cgacgccgtc
     6841 gaacaacggc tcgcggccgt gcccttccag atccactcgc atacagggcg aaccggtcca
     6901 gcccgccagg gcacggacca gggcgctgag cagcgcgtcg tcgatgcggc tgtggtagac
     6961 cgacggcacg gtctggagca ggtccgtggt ctcttcggcg ctcagctcga aagagaccgt
     7021 ggcctcgtca ccgatcagat ttccggccgc tgcttcatcg gaggggaaat ccaccggcag
     7081 gcggggcacg gcgacccgga tggtctcgcg ccagtgctcc agctcactgg ccagcgtctc
     7141 ggcatgcccg ttcagtcggc gagcccactc ctggaacgag gtggtcttcg gcggcaacgt
     7201 gacctggcga tacgccgact ccaggtcttc gagcagcacg cgccaggaga tcccgtccac
     7261 caccaggtga tgggtgaccc agagcagccg cgaaggctgg cctccggcct cgaacaggca
     7321 gaggcgcgtg agcggtccgc tcgccaggtc gaagcctgcc tgcagcgccg cggccgcccg
     7381 ctcgaaagcc tcgtcccggc tcgtcgccgg caggcccgag aggtcgacct ggtggaaggg
     7441 cgctgtcgtc gcggcgggct cggccggcgc attctcctgt ctctcaccat cgaaccgcat
     7501 gcgcagtgcg tcatggtgct ccacgatgac agccatggcg cggtccaggg cggccggggc
     7561 gagcggctcg cgcgcctcca gcaggagggc ctggttgaag tggtgcgggt ccgcgaaatc
     7621 ctgctcgaag aaccagcgct ggatcggcgt gagcggcacc tcgcccgtca ccgccccctg
     7681 ctcggcaccg aggccatcgg tggcatccgt gtgcgtgacg tggcgggcga gaccggccac
     7741 ggtctggtgc tcgaagatct gccgcacggt gaagctcagg ccggcctggc gtgcccgcgc
     7801 cacgatctgg acggagagga tcgaatcgcc gcccagctcg aagaagttgt cgttcactcc
     7861 cacccgcggc aggcgcagca cctgcgccca gaccgccgcc aggatctcct cctcgcgggt
     7921 acgcggcgcc acgtaaccct cacaggcgcc tgccggttcc ggagccggca gggccttgcg
     7981 gtccaccttg tcgttggcgg tgaggggtaa cgccgcgagc ttcacgaagg cggacggcac
     8041 catgtagtcc ggcagccgct cgcccaaaga ccgtcgaagc tcctccaccg cgaagtcgcc
     8101 ggccacatag gccaccaacc gcgactcgcg cagcaccacc accgcctcgc ggactccggg
     8161 cagggcggcg agcgccgcct cgatctctcc gagctcgatg cggaagccgc gcaccttgac
     8221 ctgatgatcc aggcggccca agaaatcgag ggagccgtcg ggcagccagc agacgagatc
     8281 gcctgtgcgg tacagcctgt cgccgggccg gccgccgaac ggatcgggca cgaaacgctc
     8341 ggcggtcaga ccgggccggc gcaggtaacc gcgcgacaca cccgtccctc ccaccagcag
     8401 ctcgcccgtc acgccgaccg gcaccggccg cagctccgcg tccacgacat acagccggct
     8461 gttggcgatc ggccggccga tcggcacgcg gtgcggctcc tgtccgggct cgcagaccca
     8521 gcgcgtcgta tcgatcgtga cctcggtcgg cccgtacgtg ttgtccaggg gaacgggcag
     8581 acgcgccagc gcccggtcgc gcagctcggg ggtgagcgtc tcgccacccg cgatgatctg
     8641 gcgcaacgag acgcactgct ccacgccctc ctcggcgagg aaggcagaca gcatggaggg
     8701 gacgaagtcg ataaaagtga tctgatgctc gcggatggcg cgcgccaggt acgtagcgtc
     8761 gccctggctt cccgggtcgg ccaggaccag ccgcgctccg gaaatcagag gagcgaagca
     8821 ctcccagact gaaacatcga aaccgagcga ggccttctgg aggaaagcgt cttgctcatc
     8881 gattccgtag gcctgcttcg cccagcgcag ccgattgcag atcccgcggt gcgtcagcat
     8941 cacacccttc ggctgaccgg tcgagcccga ggtgtagatg acgtaggcca ggttgccggc
     9001 ggtcacgccg gaatgcagcg gctggcccat gtcttccccg gcgtcccagc gcgcctcatc
     9061 caaaaggacc agtgagaggc cctccgccgg cacccgatcg cggagagact cctgagccag
     9121 cagcacccgg gcgtccgcgt cgtcgatcag gaaggccagc cgctccgcgg gataggcggg
     9181 atcgagcggc agccaggcgc cgccggcttc gaggatggcg agcatgccca cggccatggc
     9241 gggcgagcgc tcggtgtaga ggccgacgat cgaatccggg ccgacgccca gacccctgag
     9301 gtggagcgcc agacggcgcg cgctgccgat caggcggcga taggtccagc gctcggctcc
     9361 catctcgacg gccacggccg acggcgtccg cgccgcctgc gccgccactt ctaggtggag
     9421 acaactctcc ttcgcgaaga cctcggcgcg cgtgtcgttc cactcacgca ggatctgggc
     9481 gctctcggcc tcgctcgcga ggggcagatc gaaaaccggc ttgtccggct cggcggccgc
     9541 cgcggcgagc agccgttcga aatgaccggt gagacggtcg atggtcgcgg cgtcgaacag
     9601 atcggcggca tgctccatcg tgccgccgag cccgccgtcc ctctcctcga gggcgagggt
     9661 gagatcgaac ttcgccgttg ccaccgtatc gccggcggga cgcagactca caccctggag
     9721 gtcgaggttc ccgatgggga cgttctgcag ctcgaacatc acctggaaca gtggcgtctg
     9781 ggcgaggctc ctctccggcg cgagctcgtc gaccagcttt tcgaacggca cgtcctggtg
     9841 caggtaggcg gccagcgcgg tctcgcgcac ccgcccgagc aactcgcgga acgtgggcgc
     9901 gcccgccctg cccccaggca ggtttccagt caaatctccg cgcaggacca gggtgttgac
     9961 gaacaatccg atcaatccct cggtctcgac ccggttgcgg ccggcgaccg gggtgccgac
    10021 cgcgagatcg tcctggccgc tgtagcgggc gagcagggtc tggaaggcgg cgagcagcgc
    10081 catgaagagc gtcacccctt cgcgccggcc cagggcttcg acctgccggg tgagcgcggc
    10141 gggcagccgc accggccggg tggcaccccg gtgactcagg accgcgggcc gcgggcggtc
    10201 ggtgggcagc tcgaggagcg ccggcagccc ggcgagctgt tggcgccaga aatcgatctc
    10261 gttttccaga acctcgccgc gcagccagga gcgctgccac accgcgtagt ccgcgtactg
    10321 caccggcagc tccggcaaag gtgacggcct cccctcggcc aacgccggat agagcgccgc
    10381 gatctcacgg accaggatcc ccatggacca gccgtcgctg acgatgtgat gcagggtcag
    10441 caggacgaca tgatccatct cgccgagacg caacagggag ccgcgcagaa gcgggccgcc
    10501 ggtgagatcg aaggggcggg cggcctcctc gccggtcagg ctgacggcca gcgtctcgcg
    10561 ctcgctatcc ggcagggccg acaggtccac gaccggcagc aggaagggtc cggccggttg
    10621 gatcacctgg accggcgagc cctccggcgc ggcgaagacc gtgcgcaacg cctcgtgccg
    10681 gcgcacgatc tcctcgagac agcgggccag caccgcgccg tccagcggcc cttcggctct
    10741 cagcgccccg ggcatgttgt agagcgggct gcccggctcg agctgatcga tgaaccagag
    10801 ccgctgctgc gcgaacgaca ggggcaggtc cccctcgcgc ggcaccgcga ggagaggcgg
    10861 tgcggacgcc gccgtgcccc gcgccgcttc gacccggccg gcgaagtccg ccagccgcgg
    10921 ggcggcgaag aggtcacgca acggcacttc gacgcccaac gtgccgcgca ggcgcgatat
    10981 cacccgggtc gccagcagcg agtgcccgcc cagctcgaaa aagtgatcgg ttgctcccgc
    11041 cctctccagc ccgagcacct cggcccagat accggcgagg atctcttcca tcgccgtgcg
    11101 cggcgccacg aagctctctt cggcgcggcc ccattccggt gccgcacccc catccaacag
    11161 cgccttgcgg tccaccttgc cgttaggagt gagaggcagc gccgggagca tcaccagggc
    11221 ggacggcacc atgtactccg gcagccgctc gcgcagcccc tggagtagcg cctccttctc
    11281 ttcggtaccc actacatagg cgaccagacg ccggtcgccg gacgggctgt tgctcgcgac
    11341 gacgaccgcc tgacggactc cgggcagcgc ggccagcacc gtctcgatct ctccgagctc
    11401 gatgcggaag ccccgcacct tcacctgatg atcgatccgc cccagcacct ccatccgccc
    11461 gccggtcagg tggcgggcca ggtcgcccgt cctgtaaagg cgttctcccg gctccgccgc
    11521 cagcgggttc ggcaggaagc gatcggccgt caggtccggc cggcccagat agccccgcgc
    11581 cagacccgcg ccgccgatcc acagctcgcc ggtcgcgccg agaggcacgg gctggagatg
    11641 gccgtcgagc aggtggatcc gggtggcggc gatgggccgg ccgatcgaga ccggaccggt
    11701 ctccccggcc cggacgcgcg ccgccgccga ccag
//
LOCUS       DASPJI010000265         8036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_821239, whole genome shotgun sequence.
ACCESSION   DASPJI010000265 DASPJI010000000
VERSION     DASPJI010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821239"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VEL74_06965"
     CDS             <1..446
                     /locus_tag="VEL74_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62304.1"
                     /translation="PAAPAAPAVSGAMMYMDLYSYDECQLTPTITTSHSIEIGPGELD
                     IHPAARWARLQKNLVLYDYVTGVSTPATIDFTWTATGEPTRSTYRQSRVSPYSRFTAH
                     YTGVTRDAQLAGTISSGAVNLIAQTSDGWGSISHSTTGSMTRYYQ"
     gene            473..1084
                     /locus_tag="VEL74_06970"
     CDS             473..1084
                     /locus_tag="VEL74_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62305.1"
                     /translation="MKRIVFIALAVSSVLLLSTVAQAAPPTKYRIQDEAVNAELYYYD
                     PAACVVTEVHVGAHETVEQQVGGPVHQRWLSVDLYTFNDCTFSSSFAEGYVQLNSGEL
                     EVLGSAKSARLQKSLVLQNNYTGDAVPVTLDLTWTAFGEPYRNSYHQANHSQFVRTSW
                     NYTGVARDAEVTGSVTSGTTHLLPQSSIHGGTISTSNSGAVLH"
     gene            complement(1157..1909)
                     /locus_tag="VEL74_06975"
     CDS             complement(1157..1909)
                     /locus_tag="VEL74_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYG62306.1"
                     /translation="MSQTFYYGHDLAYLHHAGHGDFARQVAPNLIRLLRASGVPGGLV
                     VDLGCGSGILAHELTRAGYDVLGVDLSPEMIKLARKNAPQARFITGSLYEVDLPSCDA
                     VIAIGEVFNYIPPVAEQHPRLSTQFFKIAQALRKGGVLLFDILVQATVKNVTYDKWSE
                     GEDWAAHVTTHEDRNARTVDRHITLFRQIGELYRRSNETHRLYMHWRSQIERDLRQFD
                     FAYKYSHRYDDYVLLPRRLAFHATKGRPKGPS"
     gene            complement(1906..2448)
                     /locus_tag="VEL74_06980"
     CDS             complement(1906..2448)
                     /locus_tag="VEL74_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006607729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYG62307.1"
                     /translation="MRVLNIHERDLDATPAEVGRLIDSLSSPADVLWPSRHWPAMRFD
                     RPLQVEATGGHGPIRYFVEAYEPGRRVQFHFTGPEGFLGFHAFVVQEIAPGRTRLRHV
                     LEMETVGPAVRTWPLVFRWLHDALLEDCLDQAETATGGKPRARHWSPWVRLLRSVLRA
                     RRRSSRPIGDLAAKGPQTRT"
     gene            2572..3036
                     /locus_tag="VEL74_06985"
     CDS             2572..3036
                     /locus_tag="VEL74_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62308.1"
                     /translation="MSGIDPVTINQYWMTERSMPEYEEPAYQWATGRGLVTRPVVFRD
                     AAGAFWKPKAGFDLVVFHPRSQYENGRPAWLRLGGLRSPYLLPMWICGPAKRCLVHAR
                     LAAEGKDAIPVDQVLVSAGKPAPALMLPKGDFVVRVEGADGALIDRMPVRMP"
     gene            complement(3056..3499)
                     /locus_tag="VEL74_06990"
     CDS             complement(3056..3499)
                     /locus_tag="VEL74_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62309.1"
                     /translation="MDRKRSFLLIVLLALGGCQAETEQAARPAPPAPEPAAAVQEAPP
                     AGPPFAGKVWVSTTSPDLPGVMRIFLADGTLVMDSCWETYRLARWRMASDHELVWEED
                     SQEIRADVESSGEELALRLHLVDGTTREERYRPASVPFVCPDMKR"
     gene            complement(3503..5920)
                     /locus_tag="VEL74_06995"
     CDS             complement(3503..5920)
                     /locus_tag="VEL74_06995"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HYG62310.1"
                     /translation="MKRTPLPVLLAVLLLVLLFQHPAPVPARPDPAAELATRAKAVLA
                     RTSGRIRLPGLQKPVTVLRDPWGVPHIFAETQDDLFFAQGFVAAQDRLWQMDMWRRSG
                     EGKLAEVLGPRAVDRDRFARLVRYRGDMEAEYASYAPDAKSILQAFVRGINAFIDSSG
                     DRLPIEFQLTGIRPEPWTPEVCLNRLAALGVTGNAGAEIGRAELARQMGTAFVDAVLP
                     SEPFRELQIPEGLDLADVDAKVLTLMHAAGAPLSFTEERDGSNNWVVDGTLSATGKPL
                     LANDPHRTIALPSLRYMTHLVGPGWNVIGAGEPALPGVAAGHNDRVAFGFTVVGIDQQ
                     DLYAEETHPEDPNRYLHQGRWEPMRIEREVIRVKGEKPREVELKFTVHGPVIHEDRER
                     RRAWALRWVGSEPGTAGYLASLSLNRARTWPEFVKALERWKVPSENLVYADVDGNIGW
                     RVAGLAPVRRGWNGLLPVPGDRPGSVGRYEWQGFLPASELPGAFNPPSHFLATANHNI
                     LPPGYPHELGYDWAPLDRIGRINEVLGVPGRKFTVADFERLQHDELSHDACVAIDLLE
                     KARNVEEDLRPFVGMLARWDCVLGKDSAPAALYGLWRLKLTPAVFKPRVPEGGWKLLE
                     GRLPPDVALAVLQKPTPRWFGDDPAAGRDAALVSSLREAVAEVREKLGADPAKWRWGA
                     IHKAPFRHVLSTDDVRRSLFDLPSPERGGNGSTVNATGGGGLLQSHGASFREVIDVAD
                     WDRSTATSVPGQSGQPGSPHYGDLLPLWAEGRYFPLLYTREAIEKAAKQRLVLEPAPG
                     PKAETRR"
     gene            complement(5941..7155)
                     /locus_tag="VEL74_07000"
     CDS             complement(5941..7155)
                     /locus_tag="VEL74_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase family
                     protein"
                     /protein_id="HYG62311.1"
                     /translation="MPRHEVLALILAGGQGGRLEVLTEERAKPALPFAGVYRLIDFSL
                     SHCVHSRIPDVWVVEQYEPHSLNEHLSNGRPWDLDRTRGGLQVLPPYQQRSRDGDGGF
                     SAGNADAIYRHRGFLRELDPELLLVLSADHIYKLDFREVIRTHREHEAGVTMVTTRVP
                     REEAGRFGVVETDSDGRVTGFAYKPDEPESDIVTAEVFVYRTRKLLDTLDQIASEAED
                     GEAPLKDFGHELIPQLVEEGCAWEHRLDGYWKDVGTVESYFQAHMDLLDPEPALALDD
                     PDWPILTVGHQRLPARILEAARIDDSLVSPGARVEGRVVRSVLGPGVVVEKDAEIRDS
                     ILLDDTVVRSGARVHRSIVDADVRVGDGAVVGGAGEGAVEITLVGRRARIASGARVEA
                     GARIGPRVRIGG"
     gene            7250..>8036
                     /locus_tag="VEL74_07005"
     CDS             7250..>8036
                     /locus_tag="VEL74_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG62312.1"
                     /translation="MSALVRELQQAIRRMARSPALAMAAILCTALGVGVVSAGWIAVD
                     AVLLRPLSYVDPDRLVVLWERALDGSAERIVVSPANFGDWRERTVQRGQRGSFERLAA
                     FNVWFPGLTGVERPEKLLGAMVSADFFPVLGARPLLGRTLLPEDDRPGAAPVVVLGHG
                     LWRSRFGGDREVLGRAIELDGVPHTVVGVMPEGFRHPEPLYLDETTALWKPLALDTSE
                     PARAQRFLRVVGKLPPGVPLSRAQAEMDEVARRLAREHPAENEG"
BASE COUNT         1219 a         2797 c         2787 g         1233 t
ORIGIN      
        1 ccccggcggc cccggcggcc ccggcggtga gcggggccat gatgtatatg gacctctaca
       61 gctatgacga gtgccagctc acccccacca tcaccacctc ccactccatc gagatcggcc
      121 ccggcgagct cgacatccat ccggcggccc ggtgggcccg gctccagaag aacctggtcc
      181 tctatgacta tgtgacaggt gtctccacac cggccacgat cgatttcacc tggaccgcca
      241 cgggtgagcc cacccgctcc acctatcgcc agtcgcgcgt ctcaccctac agccggttca
      301 ccgcgcacta caccggggtc acgcgggatg cccagttggc gggcacgatc agcagcggcg
      361 ccgtcaacct gatcgcgcag acgtccgacg ggtgggggtc gatctctcac agcaccacgg
      421 gctcgatgac ccgctactat caatagccgc cctcaccgag aggaaatcga tcatgaaaag
      481 aatcgttttc atcgcgcttg ccgtatcgtc ggtcctgctc ctgtccacgg tcgcgcaggc
      541 cgcgccaccg accaagtacc gcatccagga cgaagcggtc aacgcggagc tctactatta
      601 cgatccggcg gcctgtgtcg tgaccgaagt ccacgtcggt gcccacgaga ccgtcgagca
      661 gcaggtcgga ggcccggtgc accagcgttg gctgtcggta gacctctaca ccttcaacga
      721 ttgcaccttc agcagcagct tcgcagaggg ctatgtccag ctcaacagcg gcgagctcga
      781 ggtcctgggc agcgcgaaga gcgcgaggct ccagaagagc ctggtccttc agaacaacta
      841 caccggtgac gcggtccccg tgacgctcga tctgacctgg accgccttcg gcgagcctta
      901 ccgcaactcc tatcaccagg cgaaccactc ccagttcgtc cgcacctcct ggaactacac
      961 cggcgtcgcg cgggacgccg aggtgacggg cagcgtcacc tccggcacca cgcacctgct
     1021 cccgcagtcg tcgatccatg ggggaacgat ctccacctcc aacagcggcg cggtgctcca
     1081 ctagtcatcg gccgatgcgg tcataggcga cggcccggct ccgcgcggag ccgggccgtc
     1141 gtcatcagat ccggagctaa ctcggaccct tgggccggcc tttggtggcg tgaaaagcga
     1201 ggcgccgggg gaggaggacg tagtcgtcgt agcgatgcga gtatttgtag gcgaagtcga
     1261 actggcggag atccctttcg atctgggagc gccagtgcat gtaaaggcgg tgcgtctcat
     1321 tggagcggcg atagagctcg ccgatctgac ggaacagagt gatgtggcgg tcgacggtgc
     1381 gggcgttccg gtcctcgtgg gtggtgacgt gcgccgccca atcctcgccc tcgctccatt
     1441 tgtcgtaggt gacgttcttg accgtggcct ggacgaggat gtcgaagagc agcaccccgc
     1501 ccttccgcag ggcttgggca atcttgaaaa attgagtgga gagccgcgga tgctgctcgg
     1561 ccaccggagg gatgtagttg aagacctcgc cgatggcgat caccgcgtca caggagggca
     1621 gatcgacctc gtagagcgag ccggtgatga agcgagcctg gggggcattt ttgcgcgcca
     1681 gcttgatcat ctccggcgag aggtcgacgc cgaggacatc gtaaccggcg cgcgtcagct
     1741 cgtgagccag gatgccgctg ccgcagccga gatcgacgac caggccgccg gggactccgc
     1801 tcgcccgcag caggcggatc aggttgggag cgacctgacg tgcgaagtcg ccgtggccgg
     1861 cgtgatgaag gtaggccagg tcgtgaccgt agtagaaggt ctggctcatg tgcgggtctg
     1921 cggccccttc gccgcaaggt ctcctatggg tcgagaggat cgccgccgag ccctcaggac
     1981 ggagcgcagg agtcgcaccc agggggacca gtgacgcgcc cgcggctttc cgcccgtggc
     2041 ggtctcggcc tgatcgaggc agtcctcgag cagtgcgtcg tggagccacc ggaacaccag
     2101 cggccaggtg cgcacggccg gccccaccgt ctccatctcc agcacgtgcc ggagccgggt
     2161 gcgtccgggc gcgatctcct ggaccacgaa ggcgtggaac cccaggaacc cctcggggcc
     2221 ggtgaagtgg aactggacgc gccgccccgg ctcgtaggcc tccacgaagt agcggatggg
     2281 gccatgcccg cccgtcgcct ccacctgcag cggccggtcg aagcgcatcg ccggccagtg
     2341 ccgggacggc cacaggacgt cggccggaga ggacagcgag tcgatcagcc gccccacctc
     2401 cgcgggggtg gcgtcgaggt cacgctcgtg gatgttgagg acacgcatag gggctcggcc
     2461 gacattgtag ccgacctctc ccaggggttt cctccggttc tcgtataact actggaggcc
     2521 tgcacgacga atcttccttg cccccggtgg atggccggcc atttcaaaac catgagcggc
     2581 atcgatccgg tcaccatcaa ccagtactgg atgaccgagc gcagcatgcc ggagtacgag
     2641 gagccggcct accagtgggc tacggggcgc gggctggtga cgcgtccggt ggtcttccgc
     2701 gatgccgcag gagccttctg gaagccgaag gccgggttcg atctggtggt cttccacccc
     2761 cggagccagt atgagaacgg ccggccggcc tggctccgcc tcggcggact ccgctcaccc
     2821 tatctcctgc ccatgtggat ttgcggccca gcgaagcggt gcctggtcca tgcccgcctc
     2881 gccgcggagg gaaaagacgc cattccggtg gaccaggtgc tggtctcggc cggcaagccg
     2941 gctcccgcgc tcatgctccc gaagggagac ttcgtggtcc gtgtcgaagg cgcggatggt
     3001 gcgctgatcg atcggatgcc agtgcggatg ccgtagcccg cgacacgggc cggcttcacc
     3061 gtttcatgtc cgggcagacg aacgggacgc tcgccggccg gtaccgctcc tcccgggtcg
     3121 tcccgtccac cagatggaga cgcagggcca gctcctcgcc gctgctctcc acatccgccc
     3181 ggatctcctg gctatcctcc tcccacacca gctcatggtc ggaggccatc cgccacctcg
     3241 ccaggcgata ggtttcccaa caggagtcca tgaccagggt cccgtcggcg aggaagatcc
     3301 gcatcacgcc cggcaggtcc ggggaggtgg tgctcaccca gaccttgccg gcgaagggcg
     3361 gtccggcggg cggcgcctcc tggacggcgg cggccggctc gggagcgggc ggggccggcc
     3421 gggcggcctg ctcggtctca gcctggcagc cgccgagggc caggagaacg atcaacagga
     3481 acgatctctt cctatccatc acttatctcc gggtctccgc cttgggtccg ggcgccggct
     3541 ccagcaccag ccgctgcttg gcggccttct cgatcgcctc gcgggtgtag aggagcggga
     3601 agtagcggcc ctcggcccag agggggagga ggtcgccgta gtgcgggctg ccgggctggc
     3661 ccgactgccc cgggacactg gtggctgtcg agcggtccca gtcggcgacg tcgatcactt
     3721 cacggaagga ggcgccgtgg ctctgcagga gaccgccgcc gccggtggcg ttcaccgtgc
     3781 tgccattgcc gccgcgctcc ggcgacggca ggtcgaagag ggagcgccgg acatcgtccg
     3841 tggacagcac gtgccggaag ggcgccttgt ggatcgcacc ccagcgccac ttcgccgggt
     3901 ctgcgcccag cttctcgcgc acctccgcga ccgcctcccg caggctcgag accagggccg
     3961 cgtcccggcc cgccgccggg tcgtccccga accagcgggg cgtgggcttc tggagcaccg
     4021 ccagcgccac gtccggcggg aggcggcctt ccaggagctt ccagcccccc tccggcaccc
     4081 gcggcttgaa aacggcgggc gtcagcttca gccgccagag cccgtagagc gccgccggcg
     4141 ccgagtcctt ccccagcacg cagtcccagc gcgcgagcat gccgacgaac ggacgcagat
     4201 cctcctccac gttccgggcc ttctccagca gatcgatggc cacgcaggcg tcatgcgaca
     4261 gctcgtcatg ctggagacgc tcgaagtcgg cgacggtgaa tttgcggccg ggcacgccca
     4321 ggacctcgtt gatccgcccg atccggtcga gcggcgccca gtcgtagccc agctcgtggg
     4381 gatagcccgg cggcaggatg ttgtggttgg ccgtcgccag gaagtggctc ggcgggttga
     4441 acgcgccggg cagctccgag gctggcagga acccctgcca ctcatagcgc cccactgacc
     4501 caggacggtc gcccggcacc ggcaggaggc cgttccagcc gcggcgcaca ggcgcgagcc
     4561 ccgccacccg ccagccgatg ttgccgtcga cgtcggcata gaccaggttc tcggacggca
     4621 ccttccagcg ctccagcgcc ttgacgaact ccggccaggt gcgcgcccgg ttgagggaga
     4681 gcgacgccag atagcccgcc gtccccggct cgctgcccac ccagcgcaga gcccaggcgc
     4741 ggcggcgctc ccggtcctcg tggatcaccg gtccgtggac ggtgaacttg agctccacct
     4801 cccgcggctt ctcccctttc acgcggatca cctcgcgctc gatccgcatc ggctcccagc
     4861 gtccctggtg caggtagcgg ttcgggtcct cggggtgggt ctcctcggca tagaggtcct
     4921 gctggtcgat gccgacgacg gtgaagccga aggcgacgcg gtcgttgtgg ccggcggcca
     4981 ccccggggag ggccggctcg cccgcgccga tgacgttcca gcccgggccc accaggtgag
     5041 tcatgtagcg caggctgggc agcgcgatgg tgcggtgcgg gtcgttcgcc agcagcggct
     5101 tgccggtggc ggagagggtg ccgtccacca cccagttgtt gctgccgtcc cgctcctcgg
     5161 tgaaagagag cggcgcgccc gcggcatgca tcagggtaag gaccttggcg tcgacgtcgg
     5221 cgaggtcgag cccctccggg atttggagct cgcggaaggg ttcggagggc agcaccgcgt
     5281 cgacgaacgc ggtgcccatc tgcctcgcca gctcggcccg tcctatctcc gccccggcgt
     5341 tgccggtcac tccaagggcc gccagtcggt tcaggcagac ctccggcgtc catggctccg
     5401 ggcggatgcc ggtgagttgg aactcgatcg gcagccggtc gccgctcgag tcgatgaacg
     5461 cgttgatgcc gcggacgaac gcttggagga tggacttggc gtccggggca tagctcgcgt
     5521 actcggcttc catgtctccc cggtagcgca cgaggcgggc gaagcggtcg cggtccaccg
     5581 cgcgggggcc caggacctcg gcgagcttcc cctcgccgct ccgccgccac atgtccatct
     5641 gccagagccg gtcctgggcg gccacgaaac cctgggcgaa gaagaggtcg tcctgcgtct
     5701 cggcgaagat gtggggcacg ccccaggggt cgcgcagcac ggtcaccggc ttctggaggc
     5761 cggggagccg gatgcggccg gaggtccgcg ccaggacagc cttggcccgg gtggccagct
     5821 ccgccgccgg gtccggccgc gccgggaccg gggcggggtg ctggaaaagg aggacgagga
     5881 gcaggactgc gagcaggaca ggcaaggggg tccgcttcat ggagcgggat tatccaacgg
     5941 ttaccccccg atccggacgc gggggccgat ccgggctccg gcctccaccc gggcgccgga
     6001 cgcgatccga gcccgccgcc cgaccagggt gatctccacg gccccctcgc ccgcgccgcc
     6061 caccaccgcc ccatccccca cccggacgtc cgcgtccacg atggagcggt ggaccctggc
     6121 gccggaccgc acgacggtgt catccaagag gatggagtcc cggatctcgg cgtccttctc
     6181 cactacgacg cccggcccga gcaccgagcg gaccacccgc ccctccaccc gggcaccggg
     6241 tgagaccagg ctgtcgtcga tgcgtgccgc ctccaggata cgggcaggga ggcgctggtg
     6301 cccgacggtg aggatcggcc agtccggatc gtccagggcc agagccggct ccgggtcgag
     6361 gaggtccatg tgagcctgga agtagctctc cacggtccct acgtccttcc agtagccatc
     6421 gagccggtgc tcccaggcgc acccctcctc gacgagctgg ggaatcagct catggccgaa
     6481 gtccttcaag ggggcctcgc cgtcctctgc ttcggaggcg atctggtcca gggtgtccag
     6541 gagcttgcgg gtgcggtaga cgaagacctc ggcggtgacg atgtcgctct ccggctcgtc
     6601 gggcttgtag gcgaagccgg tgacccggcc gtcgctgtcg gtctccacga ctccgaagcg
     6661 gcccgcctcc tctcggggga cgcgggtggt caccatcgtg acgccggcct cgtgctcgcg
     6721 atgggtgcgg atcacctccc gaaagtcgag cttatagata tggtcggcgg agaggaccag
     6781 caggagctcg gggtcgagct cgcgcaggaa gccacgatgc cggtaaatgg cgtcggcatt
     6841 gcccgcggag aaaccgccgt cgccgtcgcg cgaccgctgc tgataagggg gcagcacctg
     6901 gaggccgccg cgggtgcggt cgaggtccca gggacggccg ttggacaggt gctcgttcag
     6961 ggagtgcggc tcgtattgct ccaccaccca tacgtcaggg atgcggctgt ggacgcagtg
     7021 gctcagggag aagtcgatga gccggtagac tccggcgaac ggcagggcgg gcttggcccg
     7081 ctcctcggtc agcacctcca gcctccctcc ctggccgccg gcgaggatca gggccagcac
     7141 ctcgtgtcgc ggcatcgtcg tcctcctctt ctccgccgcg gcgcgcggcg ggtcccctcc
     7201 acgaccgcaa ggaccgggcc aggaactcct gccatagact acgcatccca tgtccgccct
     7261 cgtgcgcgag ctccagcagg cgatccgccg gatggcccga agcccggccc tggcgatggc
     7321 cgccatcctt tgcacggctc tcggcgtcgg cgtggtgagc gccgggtgga tcgcggtgga
     7381 cgcggtgctc ctccgccccc tttcctacgt ggacccggac cgtctcgtcg tcctctggga
     7441 acgggcgctg gacggcagcg ccgagcggat cgtggtctcc cccgccaact tcggtgactg
     7501 gcgcgagcgg accgttcagc ggggacagcg gggcagcttc gagcggctgg cggcgttcaa
     7561 cgtctggttc ccgggcctca ccggcgtcga gcgtcccgag aagctcctcg gggccatggt
     7621 ctccgccgac ttcttcccgg tcctcggcgc ccggccgctg ctggggcgga cgctcctccc
     7681 cgaggacgac cggccgggag cggcgccggt ggtggtgctg ggccacgggc tctggcggag
     7741 ccgcttcggt ggcgaccgcg aggttctggg acgggcgatc gagctggacg gtgttcccca
     7801 tacggtggtc ggggtgatgc ccgagggttt ccggcacccg gaaccgctct acctcgatga
     7861 gacgacggcg ctctggaagc cgctggctct ggatacttca gagccggcgc gagcccaacg
     7921 cttcctgcgg gtcgtgggga agctccctcc aggcgtcccc ctgtctcgtg cccaggcgga
     7981 gatggatgag gtcgcccgcc ggctggcgcg ggagcacccg gccgagaacg aggggg
//
LOCUS       DASPJI010000266         2834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_75652, whole genome shotgun sequence.
ACCESSION   DASPJI010000266 DASPJI010000000
VERSION     DASPJI010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75652"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VEL74_07010"
     CDS             <1..634
                     /locus_tag="VEL74_07010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYG62313.1"
                     /translation="QTGCGALVYSRAGYGASDPLPPPWPAEFMHHEALVTLPAVLDAL
                     EIRRAVLVGHSDGGSIALLYAGGLAAGSDRLLGLALEAPHVFVEPVTVESIAALPARY
                     QKGLREKLQRHHGANTDPLFRAWTDVWLRPEFRSWNITKVLPRVSCPVQVIQGEDDPY
                     GTVAQVDAILSGVPSPAETLLLPGCGHTPHRDKPEETLRAMAGFVRRLLS"
     gene            complement(636..854)
                     /locus_tag="VEL74_07015"
     CDS             complement(636..854)
                     /locus_tag="VEL74_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62314.1"
                     /translation="MKAKLFLVTLTLGLATLAPATAPAAPPDACTQFCWTVFCAGNQV
                     CGTYTVNGQTRCGCHDPRPFPGLEPSLD"
     gene            complement(932..2311)
                     /locus_tag="VEL74_07020"
     CDS             complement(932..2311)
                     /locus_tag="VEL74_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYG62315.1"
                     /translation="MGGSEVKWYSIVAVLLVLAPVAPTEAAGAGEQPNIVFITSDDHR
                     WDGLGAAGNPAVRTPALDKLAGQGVLFRQAITSVSQCLPSRATLVTGLPAHQHGALSH
                     EQQHNNPMRPDALSKLPTVPGLLRQAGYNTVLVGKWHLEAEPWQSGFSGVKTWLPTGG
                     ALYQDPDLAKGKNRKMSKVKGYTQAIFADDAVAFLKSGEAKKGPFLLWLAFTAPHHPF
                     APNPSHIEKHYHGQPAAELLPPGFPKDIPTGDWRRYYEAISYLDQQVGRVMEALEGGG
                     LDRPTVLVFLGDNGFMMGQRGVGAEGPAGKVVPYEGSVRVPMILWSPKLRDLAGPSDL
                     PVSTLDVPPTLLALAGLEAPASWSGRNLLPALRDPKAGPAEAFAEWADEGGRFGAQAH
                     RLVRTPRHKLILWKDPARPDELYDLAADPEEKSNLIAEPEARPVLEDLKKRLKGWMEK
                     TSDPALKWE"
     gene            complement(2372..2834)
                     /locus_tag="VEL74_07025"
                     /pseudo
     CDS             complement(2372..2834)
                     /locus_tag="VEL74_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
BASE COUNT          434 a          991 c          959 g          450 t
ORIGIN      
        1 ccagaccggc tgcggcgccc tggtctacag ccgggccggc tacggcgcct cggatccgct
       61 cccgccgccc tggcccgcgg agttcatgca ccacgaagcc ctggtcactc tgccggcggt
      121 gctggacgcg ctggagatcc ggagggcggt cttggtcggt cacagcgacg gcggatcgat
      181 cgcgctgctc tacgccggag gactcgcggc cggctcggac cggctcctcg gcctcgctct
      241 cgaagcccca cacgtcttcg tggaacccgt gacggtcgag agcatcgccg ccctgccagc
      301 ccgttatcag aaaggattgc gagagaagct ccagcgtcac catggagcga acaccgatcc
      361 gctcttccgg gcctggacgg acgtctggct gcggccggag ttccggagtt ggaacatcac
      421 caaggtgctg ccgcgcgtga gctgtccggt tcaggtgatc cagggcgagg acgaccccta
      481 tggcaccgtg gcccaagtgg acgccatcct gtcaggggtg ccatcgccgg cggagacgct
      541 cctcctcccc ggctgcggtc atacgccgca ccgggataag ccggaggaga cgctccgggc
      601 gatggcgggg ttcgtgcggc ggctgctctc ctgaatcagt cgagcgacgg ctcgaggccc
      661 gggaagggac gcggatcgtg gcagccgcag cgcgtctgtc cattcaccgt gtaggtgccg
      721 cagacctggt tgccggcgca gaagacggtc cagcaaaact gggtacacgc gtcgggcggg
      781 gccgccggcg cggtcgctgg cgccagggtg gccagaccga gagtcagggt cacgaggaac
      841 agcttggctt tcatgagcat ctccttgtcc gagaggaaaa ggccctctcg ctccttatgg
      901 tgcgccaggg gccagcccgc gtgccctctc gtcactccca cttcagcgcc ggatccgagg
      961 tcttctccat ccaccctttg agccgtttct tcagatcttc cagcaccggc cgcgcctccg
     1021 gctcggcgat caggttgctc ttctcctcgg gatcggccgc gagatcgtac agctcgtccg
     1081 gacgggccgg gtccttccag agaatgagct tgtggcgcgg cgtccggacg agccggtgcg
     1141 cctgggcacc gaagcgtccc ccctcgtccg cccactcggc aaaggcttca gcagggccgg
     1201 ccttgggatc gcgcagggcc ggcagcaggt tgcgtcccga ccacgacgca ggggcttcga
     1261 ggccggcgag cgccagcagc gtggggggaa cgtcgagcgt cgagaccggc aggtcggacg
     1321 gtccggcgag gtcccgcagc ttcggcgacc agaggatcat cggcacgcgg acggagccct
     1381 cgtagggcac caccttcccg gccgggccct ccgcgccgac gccgcgctgt cccatcatga
     1441 aaccgttgtc gcccaggaac accaggaccg tcggccggtc gaggccgccg ccttcgagcg
     1501 cctccatgac ccggcctacc tgctggtcga gataactgat cgcttcgtag tagcgccgcc
     1561 aatcccccgt cgggatgtct ttcggaaagc ccggcggcag gagctcggcg gccggttggc
     1621 cgtggtagtg cttctcgatg tgggacggat tcggcgcgaa aggatgatgc ggcgcggtga
     1681 acgccagcca gaggaggaac ggccccttct tcgcctcgcc gctcttgagg aacgccaccg
     1741 cgtcgtcggc gaagatcgcc tgcgtgtagc ccttcacctt cgacatcttc cggtttttcc
     1801 ccttcgcgag gtcggggtcc tgatagaggg cgccgccggt gggcagccac gtcttcaccc
     1861 cggagaaacc gctctgccag ggctcggcct cgaggtgcca cttgcccacc aacaccgtgt
     1921 tgtagccggc ctgccggagg aggcccggaa ccgtcggcag cttgctcaag gcgtccggcc
     1981 gcatcgggtt gttgtgctgc tgctcgtgcg agagcgcccc atgctggtgg gccggcaggc
     2041 cggtcaccag ggtggcccgg ctgggcaggc actgggagac gctggtgatc gcctgccgga
     2101 aaagcacccc ctggccggcg agcttgtcga gcgccggggt ccgcacggcg ggattgccag
     2161 ccgcgcccag accatcccag cggtggtcat ccgacgtgat gaaaacgatg ttcggctgct
     2221 ctccggcccc ggcggcttca gtcggggcga ccggagcgag gaccaacagt acggccacaa
     2281 tggaatacca cttcacttcg gaccctccca tcgacgtgtg gcgttcaggg ctccctggcc
     2341 cctcttctct tgtatacgga tggacgggag agaacgggac gttcagccac ttgtgagcat
     2401 ccaccgtgat cgaatccgcc gcctcgacgc cggccaccag atgccggaac cgtggcgagc
     2461 aggcggcgaa caggccgaag gcgccatcca cgtggagcca gaaggagtaa cgctccttga
     2521 ggcgcccgat ggcctccaga tcgtcgaagt ccgtagtgtt gaccgtgccg gcgttggcca
     2581 ccacaatgac gggccgcccc gcctcctccc gcaggagccc ttccagggcg gccacgtcga
     2641 ccgcctcccg tcccggcaga ctgggcaccc gcaccagcgt ccggcgcccc atgcccagca
     2701 tgctcatggc tttgaggatg ctggagtggg cgctcccgga gagcaccgga atgggagaga
     2761 gccccgccgt cccctcctcc gccacgtcga tcccgaggtc ccgcgccagc ttctgccgcg
     2821 cggtggcgag cccc
//
LOCUS       DASPJI010000267         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_821328, whole genome shotgun sequence.
ACCESSION   DASPJI010000267 DASPJI010000000
VERSION     DASPJI010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821328"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VEL74_07030"
     CDS             complement(<1..1162)
                     /locus_tag="VEL74_07030"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HYG62316.1"
                     /translation="MSQAYILDTLRTPRGRGKAGKGALSGVHPQELLAQTLDCLAERA
                     GIDRRQVEDVVVGCVSQVGEQGSNVARNAILAAGWPIEVTGVSLNRFCGSGLQALNFA
                     AMGVMSGAQDLVIAGGVESMSRQPMGSDLAGQDGGNVRLREKHFQVPQGISADLIATL
                     EGFSREDVDAFALESQRKAARAQAEGRFARSLFAVRDPETGKVLLEKDEHPRPDTSAE
                     GLAALQPSFAGLGASPLGPNGETYDEVALQVYPQVQEIRHVHTAGNASGIVDGAAAVL
                     LASEDYVRSRSAKPRARIKAIATAGSEPVIMLTAPAPASRKALAKAGMEPGNIDLWEI
                     NEAFAVVPLQTIKELGIDPERVNVNGGAIALGHPLGGTGAVLITKALHELERT"
     gene            complement(1263..1853)
                     /locus_tag="VEL74_07035"
     CDS             complement(1263..1853)
                     /locus_tag="VEL74_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYG62317.1"
                     /translation="MGKGAETRDRIVDSALRLASRDGFEGLTLGTLSTELGLSKSGLF
                     AHFGSKDELQLQVLQAAVNRFTETVVRPALAMPRGEPRIRAFFENWLGWANDPCMPGG
                     CILVTAAVEFDDRPGPQRDVLAAAHKDRVAFMTKAARLAVEAGHFRPDLDLGQFAFDM
                     DCIALGYHHSQRLLRDPKAGERARFAFERLLAAARA"
     gene            2060..>2968
                     /locus_tag="VEL74_07040"
     CDS             2060..>2968
                     /locus_tag="VEL74_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62318.1"
                     /translation="MKKIGILFGQEDTFPWALIERINSKGVAGITAEPVRIEKLIQGE
                     DTGYAVIIDRISQDIPFYRAYLKNAALNGTAVVNNPFWWSADEKFFNNCLATKIGVPV
                     PKTVLLPSKEHPPDTSERSMRNLAYPLDWQGIFNYVGFPVFFKPFAGGGWKNVYRLES
                     PEQFFEVYNQTGQLVMMLQEEIVFNEYFRCYCVDGRDVRIMSYDPRQPFHMRYVRNGP
                     PVAQKLLDEVHEGVLKLNRALGYDFNTVEMAVRDGIPYAIDFCNPAPDCDVHSVGEDN
                     FEWVVETVTKMVIRKAQAYEPGKNNLT"
BASE COUNT          467 a          966 c         1064 g          471 t
ORIGIN      
        1 cggtgcgctc gagctcgtgc agggccttgg tgatcagcac cgcgccggtg ccgccgaggg
       61 ggtggcccag ggcgatggcg ccgccgttga cgttcacccg ctcggggtcg atccccagct
      121 ccttgatcgt ctgcaggggc accacggcga aggcctcgtt gatctcccag aggtcgatgt
      181 tccccggctc catgcccgcc ttggccaggg ctttccggga ggccggcgcc ggcgcggtca
      241 gcatgatcac cggctcggag ccggccgtgg cgatggcctt gatgcgcgcc cggggcttgg
      301 cgctccggga ccgcacgtag tcctcggagg cgaggagcac ggccgccgcg ccgtcgacga
      361 tgccgctggc gttgccggcg gtgtggacgt ggcggatctc ctggacctgc ggatagacct
      421 gcagcgccac ctcgtcatag gtctcgccgt tcggccccag cgggctcgct cccaggccgg
      481 cgaaggaggg ctggagggcg gcgaggcctt cggcgctcgt gtccggccgc gggtgctcgt
      541 ccttctccag gaggaccttg ccggtctccg ggtcgcgcac cgcgaacagg ctacgggcga
      601 agcgcccctc ggcctgtgcc cgcgccgcct tgcgctggga ttccagggcg aaggcgtcga
      661 cgtcctcgcg cgagaagccc tcgagcgtgg cgatcaggtc ggcgctgatc ccctgcggca
      721 cctggaagtg cttctcgcgc agccggacgt tgccgccgtc ctggccggcg aggtccgagc
      781 ccatgggctg gcgtgacatg ctctcgacgc cgcccgcgat caccaggtcc tgggcgccgg
      841 acatcacccc catggccgcg aagttgagcg cctggaggcc cgagccgcag aagcggttga
      901 gcgagacgcc cgtgacctcg atgggccagc cggccgcgag gatggcgttg cgggcgacgt
      961 tcgatccctg ctcgccgacc tgggagacgc agccgacgac cacgtcctcc acctgccggc
     1021 ggtcgatgcc cgcgcgctcg gccaggcagt ccagggtttg cgcgaggagc tcctgcggat
     1081 ggacgcccga gagcgccccc ttgcccgcct tgccgcggcc gcgcggcgtg cggagtgtgt
     1141 cgaggatgta ggcctgggac atgagagaag cctccttacc gggaagagat cagagcacga
     1201 acgatcgtgc gatttttaga tgtcgagacg gggagcgtat tcatgagagt ctcggcgggg
     1261 tatcaggcgc gggcggcggc gaggagccgc tcgaaggcga agcgggcccg ctcgcccgcc
     1321 ttggggtcgc gcagcagccg ctgggagtgg tgatagccga gggcgatgca gtccatgtcg
     1381 aaagcgaact gcccgaggtc gaggtctgga cggaaatggc ccgcctcgac ggccaggcgc
     1441 gcggccttgg tcatgaaggc gacgcggtcc ttgtgggcgg cggcgaggac gtcgcgctgc
     1501 ggccccggcc gatcgtcgaa ctcgacggcg gcggtcacca ggatgcagcc gcccggcatg
     1561 caggggtcgt tcgcccagcc gagccagttc tcgaagaagg cgcggatgcg cggctcgccc
     1621 cgcggcatgg cgagggcggg gcggaccacc gtctcggtga accggttgac cgccgcctgg
     1681 agcacctgga gctggagctc gtccttggag ccgaagtggg cgaagagccc gctcttcgac
     1741 agccccagct ccgtcgacag cgtcccgagg gtcagcccct cgaagccgtc gcggctcgcc
     1801 aggcgtagcg cgctgtcgac gatccggtcg cgggtctcgg ctcccttgcc catggccgca
     1861 gtatacgaac gatcgtgcta tttcgtcaag ggggtggggc ctgggtgccc tctgtggtac
     1921 tgtatccagc cgtcccaagt ccggggcgca cctccatgca agaggaatcc gccaggttca
     1981 agcccgtgct cgccgggacg gggagccatt atctttgtca gccagtttgt cagccaggtc
     2041 gggaggagct agagagcgta tgaagaagat cggcattctg ttcgggcagg aggacacgtt
     2101 tccctgggcc ttgatcgagc gcatcaactc caagggcgtc gccgggatca ccgcggagcc
     2161 ggtgcggatc gagaagctga tccagggcga ggacacgggc tacgccgtga tcatcgaccg
     2221 gatctcgcag gacatcccgt tctaccgggc ctacctgaag aacgcggcgc tcaacggcac
     2281 cgcggtggtc aacaacccct tctggtggag cgccgacgag aagttcttca acaactgcct
     2341 ggccaccaag atcggcgtgc cggtgccgaa gaccgtcctg ctgccgtcca aggagcatcc
     2401 gccggacacc agcgagcgct cgatgcgcaa tctcgcctat ccgctggact ggcaggggat
     2461 cttcaactac gtcggcttcc cggtcttctt caagccgttc gccggcgggg gctggaagaa
     2521 cgtctaccgg ctggagagcc cggagcagtt cttcgaggtc tacaaccaga ccggccagct
     2581 cgtgatgatg ctccaggagg agatcgtctt caacgagtac ttccggtgct actgtgtcga
     2641 cggccgcgac gtccggatca tgtcgtacga cccgcggcag ccgttccaca tgcgttatgt
     2701 gaggaacggc ccgccggtgg cgcagaagct gctggacgag gtgcacgagg gggtgctgaa
     2761 gctcaaccgg gcgctcgggt acgacttcaa caccgtggag atggcggtgc gcgacggcat
     2821 cccgtacgcg atcgacttct gcaacccggc gccggactgc gacgtgcact cggtgggcga
     2881 ggacaacttc gagtgggtcg tcgagacggt gacgaagatg gtgatccgca aagcccaggc
     2941 ttatgagccg ggcaagaaca acctgacc
//
LOCUS       DASPJI010000268        16978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_478833, whole genome shotgun sequence.
ACCESSION   DASPJI010000268 DASPJI010000000
VERSION     DASPJI010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16978)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16978
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478833"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /locus_tag="VEL74_07045"
     CDS             <1..1362
                     /locus_tag="VEL74_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYG62319.1"
                     /translation="VSLDISERKRFEAQLSHLANHDALTGLYNRHRFEEELKLQLSQA
                     HRYLVHGALLWCDLDQFKDINDSLGHRAGDELLIKVATSLREQVRDTDILARPGGDEF
                     AVLLPFTSRDEAQQIATRLLDGIRRNPLIVGGKPVRTTASIGIVLYPEHGATTEELLA
                     HADVAMYQAKHEGRNRSMVFSGDREWQAQLSSGMERVGHLREALEHDLFQLYLQPIVD
                     IRTGEVARFELLLRMLGADGRILTPESFLGVAERFGLMRDIDHWVVSRAIRLIAQQWR
                     QGRDLYLEVNLSGTAFTDLEMLPWIQRELAATQIDPSRLVLEITETAAVTDLSQARRF
                     IETLKELGCQFAVDDFGVGFSSFYYLRHLPVDLLKIDGSFIENLPRDPVNQNLVKAMV
                     EMARGLGIQTVAEYVGDAETMAWLRDAGLDYAQGYYLGRPGPAWQVLEACYGPPSSAA
                     LRN"
     gene            complement(1704..2390)
                     /locus_tag="VEL74_07050"
     CDS             complement(1704..2390)
                     /locus_tag="VEL74_07050"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HYG62320.1"
                     /translation="MEKLDRDETWELLGLLFQAHPWHGVLIGDEAPEVVTSYIEIVPT
                     DTVKYEMDKRSGHLKVDRPQKFSSVCPTLYGFVPQTFCGEGIAALSRERTGKEVAAGD
                     GDPLDICVLSEKAFSHGAFMLQAIPIGGLLLLDKGEADDKIIAVLKDDVAFGHFRDVS
                     ELPDAQIERIEHYFLTYKRPPGATDNVIEIAHRYGREVAHEVIERSRQDYRARFPDLK
                     ERFLKALQRE"
     gene            2428..2670
                     /locus_tag="VEL74_07055"
     CDS             2428..2670
                     /locus_tag="VEL74_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62321.1"
                     /translation="MRWLVLWLLLAVTQALGWLASRLLPVLPAGGTAWGRAEDLAHLA
                     VIPLVQALALAVVANVRRRPPPGALPHQGSEGAGGA"
     gene            complement(2667..3377)
                     /locus_tag="VEL74_07060"
     CDS             complement(2667..3377)
                     /locus_tag="VEL74_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62322.1"
                     /translation="MARPEEETTALKTLRSRIRTTLLHLELVDACLNLCDKVAGGSEE
                     KKQPLIKTLGVGGKYETLQLPTQQYRQVTNFSRGENLELALLAVYVHFMDYLRTVLPH
                     VHQRHPLTFLFQGVAAEQGGGTPSWERVLQRYEGRGGIEELVGSLIEGTGIRIDPEAG
                     REAHAYLAMRDLYVYNGGLVDDQFARFYGNDWKTTPGNKLPKNIKIGRMAMFAVERFG
                     VELDAALRALGAQNAPRP"
     gene            complement(3449..4255)
                     /locus_tag="VEL74_07065"
     CDS             complement(3449..4255)
                     /locus_tag="VEL74_07065"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011764587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HYG62323.1"
                     /translation="MATYAIGDIHGCFRTLRRLLRRIQFDAREDRLWLVGDLVNRGPG
                     SLPVLRWAAELGDRAVTVLGNHDLHLLARAAGVAGPKRRDSLEPVLEAKDRDDLLSWL
                     RHRRLVHREDGFLMVHAGLFPEWTPVAAEAVAREVEEELRGGPALPLLESIDRKAEER
                     WQDGLRRPDRLRVALAGLAKLRTLDGKGRMCAGFSGPPEEAPRGCRPWFGIPGRRSSG
                     VRVVFGHWAALGLKLGDGVAGLDTGCAWGRELTALRLDDGALFQEPARED"
     gene            4382..5065
                     /locus_tag="VEL74_07070"
     CDS             4382..5065
                     /locus_tag="VEL74_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62324.1"
                     /translation="MSRDTRQVFCGRLFLAILILVSTGGAARAQCPSGTFVIDLTTGQ
                     SFPTPPPGRITVMSLGPTANPHCDAGWQSAVLRLDLAPPCHEAEITVDFDQPIAWTVH
                     IADSPTSDGYGGDAGTTPNNAEMWINQQAMWVTSNRTGGVTDNNLYQENLNLNYGAMK
                     FAVKNHWLSWGTPLHVLNMVGTKKLFALPDTSNPAEAYFLYVGLNRVVADLPLRSGCG
                     AQRAMITIE"
     gene            5068..6261
                     /locus_tag="VEL74_07075"
     CDS             5068..6261
                     /locus_tag="VEL74_07075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG62325.1"
                     /translation="MGQTGLLPRVVHVVLGLSVGGVQRLVEQMLRTPPAGFAASCLCL
                     DEAGSLGEALRREGADVHCLGRVPGVDLRLPRRIAAFARSRGCALLHCHQYTSWFYGV
                     PARLFQPRLRVILTEHGRAFPDRSSRARQLFTACMAPLTHRVTAVSHATAEALARVEG
                     FPRERIEVILNGVRGATPPPPGGRAEARAALGLPADAVCFVLACRFDPVKGLDGLVRA
                     MRHVAGELPRARLLLLGDGEMRAGLEALIRELGLGRHVLLPGFRSDIGRWLAAADAFV
                     LSSLDEGTSVSLLEAMAAGLPCVATRVGGNPFVVQEGETGLLVPPRDEAALAAGMLRL
                     ARDPRLRESMGRKARRRYEALFTWDGMLDVYGRMYRDLLAARRPSRGAPPGDWPGGRP
                     GPAAG"
     gene            complement(6191..6703)
                     /locus_tag="VEL74_07080"
     CDS             complement(6191..6703)
                     /locus_tag="VEL74_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYG62326.1"
                     /translation="MTTRAIDSGVRIGHVHLRVADLERALRFYCGVLGFELTQRMGQG
                     AAFISAGGYHHHLGLNTWESRGGPPPAPGTTGLYHLAILYPERRLLADALRRLIEAGI
                     PLDGVADHGVSEALYLSDPDGNGVELYWDRPESDWPRDADGGLAMFTRPLDLDDLLAS
                     LQAAPRGSDA"
     gene            complement(6715..9147)
                     /locus_tag="VEL74_07085"
     CDS             complement(6715..9147)
                     /locus_tag="VEL74_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG62327.1"
                     /translation="MDHLIQDLRFSLRVMLKSPGVTLIAVLCIALGIAANTTIFSVVN
                     AILLRPLPYADPERIAVLYETQLQRGIEEDNFSLHDLQDARASVRSFSKIEAYGPRSL
                     VFSGHDEPERVLGALVSANLFEMLGEKPIHGRLFLEEEDRPGAPGVILLSHALWQRRF
                     NSDPKIVGQTVMVNATAHTVVGVMAPHFNFPYQQEAWVPATPILHKGERVDRSLVAMA
                     RLRPGATFEQANVELEGFFKRLEREYPDSHAGWSARAVTLREDFVDTEIQLMVLTMMG
                     AVICVLLIACANVANLLLARATARQREIAVRVAFGAQRRRVIRQLLTESVVVALVGGA
                     LGILLAVWGIHLMEAAIPQENQPPYWMRFVIDGNVLLFTLGIAVVTGLVFGLAPALQA
                     AKTDLNDTLKEGGRGAGGSVLRNRLRNGLVIVEVALSLLLLVGASLFIRSFLELQSVD
                     SGFDTRPLLTMRMYLPSGIYAQEDEAAMTRRIEDVVRRIEEIPAVEAAASSPTIPLSG
                     GGDDAPVEIEGRPAQPGKEPMVFYTGVTSRFFETLGISLVKGRGFTEPEGAELSRVAV
                     VNESFAQRFWPKREAVGRRFRFPTQLDPEKAGWFTVIGVVPDLYNDEADDKPLPSAYL
                     PGPYYIARNPGILVRTRLDPAQVTAQVREAIRASDPHLPVYDILTMEEVRQLGSWQYS
                     FLGKMFTSFGVIALFLASIGVYGVLSYSVSQRLREIGVRVALGAQRGDVVRLVLRQGM
                     GLALGGIALGLFGALGVTRVVSGILYNVSATDPVSFGGISVLLATVAGIASYLPASRA
                     MAVDPLDALRSE"
     gene            9283..10086
                     /locus_tag="VEL74_07090"
     CDS             9283..10086
                     /locus_tag="VEL74_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62328.1"
                     /translation="MKRLLAIWLTLLAAFWFTRAAVSALLFQSADLSERAVFQLVTVP
                     VLQTLVVGWLTRDPGRLPLLAPLRAALAHPGVRAMLVLDLLLVLAGAVFYRNPGLAPA
                     PPSLPALWVGLKLLAAGGLLVLLSRRGDAGERLRLYVLAASLAILGVVGATGFLDWSG
                     VLLARWLPEASPMALWLFTRAPLAVAAAAALLEVQEMFRPSDPGAARALDWALGLGLL
                     AALVTVLDLFALPYPAAPWGPLVTASATLGSTALLIGLALAGGWTEARD"
     gene            complement(10092..11003)
                     /locus_tag="VEL74_07095"
     CDS             complement(10092..11003)
                     /locus_tag="VEL74_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62329.1"
                     /translation="MKRSICRAALLAALLLAGMGRASRAADLSQILSHDTGVPLDLQV
                     VGTEKRPGVTVQDVTFRSLAGGAPVQAYVVRPSEEAGPFAGVLFVHWYAPSFATSNRT
                     QYLSEAEALARRGTVSLLVSTFWSDPARYRGRRWEDDYQNTVGQAKELRRALDVLLAQ
                     PGVDKGRVGYVGHDYGAMFGALVAAVDPRPKAYVMIAGTARFSDWYLFGSASGVPEGE
                     ALAGFKARLAPLEPVNAIAQARAAVFYQFGEADRYTPREDFIALYMATPGTKRLATYA
                     SDHPMGAEIIRHDRTVWLAEQLALPGW"
     gene            complement(11034..11297)
                     /locus_tag="VEL74_07100"
     CDS             complement(11034..11297)
                     /locus_tag="VEL74_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62330.1"
                     /translation="MTEAACAKCRSTKVLPKVRVMDRGDYSLDAGELALIVYEKPEAM
                     IFKGPHEGSLFARVCGECGYTEMYLENPGEFYRLFTETQGKKG"
     gene            complement(11305..11505)
                     /locus_tag="VEL74_07105"
     CDS             complement(11305..11505)
                     /locus_tag="VEL74_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62331.1"
                     /translation="MAWILRVLSFVPGLAYLIGALMFAAGLLGLWSGLRFLVEWAFAW
                     MGLAWLATALGLGVLNRDPIEE"
     gene            complement(11561..11932)
                     /locus_tag="VEL74_07110"
     CDS             complement(11561..11932)
                     /locus_tag="VEL74_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62332.1"
                     /translation="MDNALAVLMANIWVLIPLAGIGAGMFNNYLKLRSEQRKLGADNR
                     ELEKALAEMQRRQTELLDRVQNLEAIVVSQTWDVVNDRQLSEADRERRLASTVRRELR
                     PADPESANQQRAEQLARRLQA"
     gene            complement(12357..13481)
                     /locus_tag="VEL74_07115"
     CDS             complement(12357..13481)
                     /locus_tag="VEL74_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYG62333.1"
                     /translation="MRSSWALILLLALTGCGGHTIRAKFAPNYASQGAFDFKKPPCQP
                     SPKPEPGPDEVAIRYLGAGGLYVEWQGTALLMSPFFSNPRILRVPWGRLVTDEAAVER
                     GLKPMDLSRVRAIAAGHSHYDHIGDLPLVAERFAPGARVYVNNNGAHALAPVRALTGR
                     ITSLESEAGRDWIYLKDPDQNDLPIRFRAVKSAHAPHFWGVRLLRGPLAADWTGEWQT
                     HRLLSLKDGQTFAFVIDLLSQDLKKTRFRLYYQDSANPPKQGIPRLEDCRGYDLAVLC
                     MASFYFVRKQPGTILGHLRPRHVLVTHYESFFHAAGKPVRFVTLLTDFWANRYFHRAR
                     RAMSGWEADTVGPEGDVCGPSCRSWTMPVPGEWVRFRAGA"
     gene            13518..14378
                     /locus_tag="VEL74_07120"
     CDS             13518..14378
                     /locus_tag="VEL74_07120"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYG62334.1"
                     /translation="MPPLESTLRTHDGLALIGEHHLRPGCRARVVIVHGYAEHRGRYA
                     SLAAALGEAGYECHLLDLRGHGGSGGVRGFVSRFEEYFADLDLFLTRIEEVAAGGIPL
                     ILLGHSLGGLISLGYVLQRPGRFDALAVSSPFLAPAMPVPPLQAGLAAVTSRLAPKLL
                     GPSPIRPESLSHDTEVVAAYEADPLVFKTLSPRWFMEVREAQEEVLERAGEIRLPALF
                     LIGGADPIADPERSRSVFERLGSADKHLEIYQDFLHEVFNETGRQRVIDDLVNWLDRR
                     APPVSPRPAR"
     gene            complement(14326..>16978)
                     /locus_tag="VEL74_07125"
     CDS             complement(14326..>16978)
                     /locus_tag="VEL74_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62335.1"
                     /translation="ESGMQEAQAELPPEAAALLTAERPVAALLPLLEPEPTFGVRTFS
                     FTVDEIFGLLRRQQITSGHSDLALVLPGGGVKAAYQSTVVDSLYRQGYLKNFKVEPAP
                     GSAEPLDVQYVIGTSGGALLGFFVSQLGENGPWGLSDILWKKADGSFVESTDIFGWTD
                     LLRYVSVIMSFLVLCGLIALASIPERSPFSPEPVGRGMGVRRPLMLGVLLPVLALAPL
                     LVRRVSGDTLQEHVPELEGLVYAVLAMIAMVVDQAMVRLDREREGGEPWVPPALPVAL
                     GGLLVLLPLLPWGGSRIGRMLGEPLTFGPAFAVLAPLLLLGGLILPLRTGRPAGDRAA
                     IGWMRPVLHFAAAGGLALAILFLLPRPWLDAIKMPFFLAGFLMVLVSVGANFLLAART
                     GLAERTRQRIYWGALLASSLLLVTLCWPEQAAPTRSWESLGASLGARTLDVSVGSFLL
                     GVGLLILMLGGIAWVYASRRRYHLRLTEFLVPYFVVLLHAVTVYLVLVVVIALLPDWL
                     SPLELTREFWLWLLATSLLLGGLMLLAGLGGRPTGRQPGRPSGGGRLWSAVAGYLHRS
                     FAFLSSHHPNGDVITRRFLRMAVWSVCCLGWWNLVVAPALYGNRPAFRYLSSAADQFR
                     ARAGLQGVPYRPTARFIAPANLLEKDGTRYFLFIPEGDECPSIPRRPASGAQWLSYEA
                     TFGKPAEASSSDDDCEPVPDPVFLRDVIFASGSPFPIFPAHRVEFDGQKRPLVDGGYS
                     NNVPVDVALTVSASQVLIVDSTNPLGHESPPTWLSRSAAHLLGFRGKLVENLGRLPGF
                     LFERSQQVDRLSRRDLFVVSLAPSREEPDWPPLFDFRQQTVERMERVARRDIGRRIGM
                     VQSWGRPRFQLSVPVEGRREEPGGPAS"
BASE COUNT         2570 a         5993 c         5923 g         2492 t
ORIGIN      
        1 gtgtcgctcg acatcagcga gcgcaagcgg ttcgaggccc agctcagcca tctcgccaac
       61 cacgacgccc tgaccggcct gtacaaccgc caccgcttcg aggaggagct caagctccag
      121 ctctcccagg cgcaccgcta cctggtgcac ggcgccctgc tctggtgcga cctcgaccag
      181 ttcaaggaca tcaacgacag cctcggccac cgggcgggcg acgagctgct gatcaaggtc
      241 gccacctcgc tgcgcgagca ggtgcgcgac accgacatcc tcgcccggcc gggcggcgac
      301 gagttcgcgg tcctgctgcc gttcaccagc cgcgacgagg cgcagcagat cgccacccgg
      361 ctgctcgacg gcatccgccg caacccgctg atcgtcggcg gcaagccggt ccgcaccacg
      421 gccagcatcg gcatcgtgct ctatcccgag cacggcgcca ccaccgagga gctgctcgcc
      481 cacgccgacg tcgccatgta ccaggccaag cacgagggcc gcaaccgctc catggtcttc
      541 tccggcgacc gcgagtggca ggcccagctc tcctccggca tggagcgggt ggggcacctg
      601 cgggaggcgc tggagcacga ccttttccag ctctacctgc agccgatcgt cgacatccgc
      661 accggcgagg tggcgcgctt cgagctgctc ctccgcatgc tgggcgccga cggccggatc
      721 ctcacccccg agtcgttcct cggcgtcgcc gagcgcttcg gcctgatgcg cgacatcgac
      781 cactgggtgg tgtcgcgcgc catccgcctc atcgcccagc agtggcgcca ggggcgcgac
      841 ctctacctcg aggtcaacct gtcggggacc gccttcaccg acctggagat gctgccctgg
      901 atccagcgcg agctggcggc gacccagatc gacccgtcgc gcctggtgct cgagatcacc
      961 gagacggccg ccgtcaccga cctgagccag gcgcgccgct tcatcgagac cctcaaggag
     1021 ctgggctgcc agttcgcggt cgacgacttc ggcgtcggct tctcctcctt ctactacctc
     1081 cggcacctgc cggtcgacct gctcaagatc gacggcagct tcatcgagaa cctgccgcgc
     1141 gacccggtca accagaacct ggtcaaggcg atggtggaga tggcgcgggg gctggggatc
     1201 cagaccgtgg ccgaatacgt gggcgacgcc gagaccatgg cctggctccg cgacgccggc
     1261 ctcgactacg cccagggata ctacctcggc cgccccggcc ccgcctggca ggtcctcgag
     1321 gcctgctacg gccccccttc ttcagccgct ttgagaaact gagggtcctt ccgaggcgcc
     1381 cggagaccta tggctcagct tctctgagaa atttttaggt cgatcctgag gactcttgag
     1441 ggcaccggcc gctatgctct cctcaccttg aagttatctg attctgctgc tggtctcttg
     1501 tggggaaggt cggaagttct ttgaagtcta tctgatttgt tgttctgagg agggagtggt
     1561 cgagctgttt ttctcttctg ctgtcatgta gcgagagtgg tgaggcaaaa agtgcaggga
     1621 tccgtcctga aggcggagca cgagcccggg gaaccgtacc cgggcttttc ttttttacac
     1681 ggctcgaaag ggagagcggg gcttcactcc cgctggaggg ccttgaggaa ccgctccttg
     1741 aggtccggga agcgcgcccg gtagtcctgc cggctgcgct cgatgacctc gtgggcgacc
     1801 tcccgaccat agcggtgggc gatctcgatg acgttgtccg tggcccccgg cggccgcttg
     1861 taggtcagga agtagtgctc gatgcgctcg atctgggcgt cgggcagctc gctcacgtcc
     1921 cggaagtggc cgaaggcgac gtcgtccttg agcacggcga tgatcttgtc gtcggcctcc
     1981 cccttgtcga ggagcagcag cccgccgatg gggatggcct ggagcatgaa ggcgccgtgg
     2041 gagaaggcct tctccgacag gacgcagatg tccagggggt cgccgtcgcc cgcggcgacc
     2101 tccttgccgg tgcgctcgcg ggagagggcg gcgatcccct cgccgcagaa ggtctggggc
     2161 acgaagccgt agagggtggg gcagacgctg gagaatttct gcggccggtc caccttgagg
     2221 tggccgctcc gcttgtccat ctcgtacttg acggtatcgg tggggacgat ctcgatgtag
     2281 gaggtcacca cctccggcgc ctcgtcgccg atgagcacgc cgtgccaggg gtgcgcctgg
     2341 aacagcagcc ccagcagctc ccaggtctcg tcccgatcca gtttttccat ggcgcgagga
     2401 tacacgagcc tcgggtagga tcaccccatg cgatggctcg tcctctggct gctcctcgcc
     2461 gtcacccagg ccctgggctg gctcgcctcg cgcctgctgc cggtgctgcc ggccggcggc
     2521 acggcctggg ggagggccga ggacctcgcc cacctcgcgg tgatcccgct ggtgcaggcc
     2581 ctcgccctcg cggtggtcgc caacgtccgg cggcggccgc cgccgggcgc cctcccccac
     2641 caggggagtg aaggcgccgg gggagcctag ggccgggggg cgttctgggc ccccagggcg
     2701 cgcagggcgg cgtcgagctc gacgccgaag cgctcgacgg cgaacatggc catgcggccg
     2761 atcttgatgt tcttgggcag cttgttgccg ggggtggtct tccagtcgtt gccgtagaag
     2821 cgggcgaact gatcgtccac caggccgccg ttgtagacgt agaggtcgcg catcgccaga
     2881 taggcgtggg cctcgcggcc ggcctcgggg tcgatccgga tccccgtccc ctcgatgagg
     2941 ctcccgacca gctcctcgat gccgccgcgc ccctcgtagc gctgcagcac ccgctcccag
     3001 gagggcgtgc cgccgccctg ctcggccgcc acgccctgga agaggaaggt cagcgggtgg
     3061 cgctggtgga catggggcag caccgtgcgc aggtagtcca tgaagtggac gtagaccgcc
     3121 agcagggcga gctccaggtt ctcgccgcgg gagaagttgg tgacctgccg gtactgctgg
     3181 gtgggcagct gcagggtctc gtacttgccg ccgacgccca gggtcttgat gaggggctgc
     3241 ttcttctcct cggagccgcc cgccaccttg tcgcagagat tgagacaggc atcgaccagc
     3301 tcgagatgca gcaaagtggt ccggatccgg gaccgtagag tcttgagggc cgtggtttct
     3361 tcctcaggcc gcgccattcg acctccttta tcgctaacgc catgcgagtt tgaacgatgc
     3421 gtagcctatc aaaccgcaag gccccgcgtc agtcctcccg cgccggctcc tgaaacaggg
     3481 cgccgtcgtc caggcgcagc gcggtcagct cgcggcccca ggcgcagccg gtgtcgaggc
     3541 cggcgacgcc gtccccgagc ttgagcccca gggcggccca gtggccgaac accacgcgca
     3601 cgccggagct ccggcgcccc gggatgccga accaggggcg gcacccccgg ggcgcctcct
     3661 cgggcgggcc gctgaagccg gcgcacatgc gccccttgcc gtcgagggtg cgcagcttgg
     3721 cgaggccggc gagcgcgacg cgcaggcggt ccgggcggcg gagcccgtcc tgccagcgct
     3781 cctcggcctt gcggtcgatc gattcgagca gggggagggc cggtccgccg cggagctcct
     3841 cctccacctc ccgcgccacc gcctcggcgg cgacgggggt ccactccggg aagagaccgg
     3901 cgtgcaccat caggaagccg tcctcgcggt gcaccaggcg ccggtgccgc agccaggaca
     3961 gcaggtcgtc gcggtccttg gcctccagca ccggctcgag gctgtcgcgc cgcttcggcc
     4021 cggcgacccc ggcggcccgg gcgagcagat ggaggtcgtg gttgcccagc accgtgaccg
     4081 cgcggtcccc gagctccgcc gcccagcgca gcaccggcaa cgaccccggc ccgcggttga
     4141 ccaggtcccc caccagccag agccggtcct cccgcgcgtc gaactggatg cggcgcagca
     4201 gccgccggag cgtccggaag cagccgtgga tgtcgccgat cgcgtaggtc gccatgcggc
     4261 aacgatgtcg cggggccccc tggaggtcaa ggggaggatc gagagggcca ggggcaatat
     4321 ctcgcatgat tattgcattg gcttaagttc gtcaacgtcc actctttccc aggaggtcca
     4381 catgagtcgt gacacccggc aagttttctg cggtcgtctg ttcctggcca tcctgatcct
     4441 cgtgtccacc ggcggcgcgg cccgggcgca gtgcccctcg ggcacgttcg tgatcgacct
     4501 gaccaccggc cagtcgttcc ccaccccgcc tcccggccgc atcacggtga tgtcgctggg
     4561 cccgacggcc aatccgcact gcgatgccgg ctggcagtcg gcggtgctgc gcctggacct
     4621 cgccccgccc tgccacgagg cggagatcac ggtggacttc gaccagccca tcgcgtggac
     4681 ggtgcacatc gccgactcgc cgaccagcga cggctacggc ggcgacgccg gcaccactcc
     4741 caacaacgcc gagatgtgga tcaaccagca ggcgatgtgg gtgaccagca accgcacggg
     4801 gggcgtgacc gacaacaatc tttaccagga gaacctcaac ctcaactacg gcgcgatgaa
     4861 gttcgcggtg aagaaccact ggttgagctg gggaacgccg ttgcacgtcc tgaacatggt
     4921 cgggacgaag aagctcttcg ccctgccgga caccagcaac cccgccgagg cctacttcct
     4981 ctatgtggga ctcaaccggg tggtggccga cctccccctg cgcagcggct gcggcgccca
     5041 gcgcgccatg atcaccatcg agtagtcatg gggcagacag gtctgctccc tcgggtcgtg
     5101 catgtggtgc tcgggctgtc ggtgggcggg gtgcagcgtc tggtggagca gatgctgcgc
     5161 acgccgccgg cggggttcgc cgcctcctgt ctctgtctcg acgaggccgg ctccctgggg
     5221 gaggcgctcc gccgggaggg cgccgacgtc cactgcctgg ggcgcgtacc cggggtcgac
     5281 ctccggctgc cgcgccggat cgcggccttc gcgcgctccc gcggctgcgc cctcctccac
     5341 tgccaccagt acacgtcctg gttctacggc gtcccggccc ggctcttcca accccgcctg
     5401 cgggtgatcc tcaccgagca cgggcgcgcc ttcccggacc gctcctcccg ggcgcggcag
     5461 ctcttcaccg cctgcatggc cccgctcacc caccgcgtca cggcggtctc ccacgccacc
     5521 gccgaggcgc tggcccgggt ggaaggcttt ccacgggagc gcatcgaggt gatcctcaac
     5581 ggcgtgcggg gcgccacccc accgccgccc ggaggccggg ccgaggcgcg ggccgccctg
     5641 ggcctgccgg cggacgccgt ctgcttcgtc ctggcctgcc gcttcgatcc ggtcaagggg
     5701 ctcgacgggc tggtgcgggc gatgcgccac gtcgccggcg agctgccgcg ggcacggctg
     5761 ctcctgctcg gcgacgggga gatgcgagcc ggtctcgaag cgctgatccg ggagctgggc
     5821 ctcggccggc acgtgctcct ccccggcttc cggtccgaca tcggccgctg gctcgccgcg
     5881 gccgacgcct tcgtgctcag cagcctcgac gaggggacca gcgtcagcct gctcgaggcc
     5941 atggccgcgg gcctgccctg cgtcgccacc cgggtcgggg ggaacccctt cgtcgtccag
     6001 gagggagaga cggggctgct ggtgccgccc cgcgacgagg ccgcgctcgc cgccgggatg
     6061 ctgcgcctgg cccgagaccc ccggttgcgg gagagcatgg gacgcaaggc gcggcggcgc
     6121 tacgaggcgc tcttcacctg ggacggcatg ctggacgtct atggtcggat gtaccgcgac
     6181 ctgctggcgg ctaggcgtcc gagccgcggg gcgccgcctg gagactggcc aggaggtcgt
     6241 ccaggtccag cggccgggtg aacatcgcca gaccgccgtc ggcgtcgcgc ggccagtcgg
     6301 actccggccg gtcccagtag agctcgacgc cgttgccgtc cgggtcgctc aggtagagcg
     6361 cttcgctcac cccgtgatcg gcgacgccgt cgagggggat tcccgcctcg atcagccgcc
     6421 ggagggcgtc ggccagcagc cggcgctccg gatagaggat cgccaggtgg tagaggccgg
     6481 tggtccccgg cgccggcggc ggcccgcccc ggctctccca ggtgttgagc ccgagatgat
     6541 gatggtagcc gccggcgctg atgaaggcgg caccctggcc catccgctgg gtgagctcga
     6601 agccgaggac gccgcagtag aaccgcagcg ccctctcgag gtccgccacc ctcaggtgga
     6661 catggccgat ccggactccg gagtcgatgg cgcgggtcgt cataccgtca cagtctactc
     6721 cgaccgcagc gcgtccagcg ggtccaccgc catcgcccgg gaggccggca ggtagctggc
     6781 gataccggcg acggtggcca gcagcaccga gatgccgccg aagctcaccg ggtccgtggc
     6841 gctgacgttg tagaggatgc cggagaccac gcgggtgacg cccaaggcgc cgaagaggcc
     6901 gagggcgatg ccgcccaggg cgagccccat cccctgccgc agcaccaggc gcaccacgtc
     6961 gccgcgctgg gcgccgaggg ccacccgcac gccgatctcc cgcagccgct ggctcaccga
     7021 gtaggacagc acgccgtaga cgccgatcga ggcgaggaac agggcgatca cgccgaagga
     7081 ggtgaacatc ttgccgagga agctgtattg ccaggagccg agctgccgca cctcctccat
     7141 ggtgaggatg tcgtagaccg gcaggtgcgg gtcggaggcc cggatcgcct cgcgcacctg
     7201 ggccgtcacc tgcgccggat cgagccgcgt ccgcacgagg atcccggggt tgcgggcgat
     7261 gtagtagggg cccggcaggt aggcgctcgg cagcggcttg tcgtccgcct cgtcgttgta
     7321 gaggtcgggg acgacgccga tgacggtgaa ccaccccgcc ttctccggat cgagctgggt
     7381 ggggaaccgg aaccggcgcc cgaccgcctc gcgcttcggc cagaagcgct gggcgaagga
     7441 ctcgttgacg accgcgacgc gggaaagctc ggccccttcc ggctcggtga agccgcgccc
     7501 cttcaccagg gagatgccca gggtctcgaa gaaccgcgag gtcacgcccg tatagaagac
     7561 catgggctcc ttgccgggct gggccggccg cccctcgatc tcgaccggcg catcgtcgcc
     7621 gccgccgctc aggggaatgg tgggagagga ggccgcggcc tcgacggccg gaatctcctc
     7681 gatccgccgg accacgtcct cgatccggcg cgtcatcgcc gcctcgtcct cctgggcata
     7741 gatcccggac ggcaggtaca tcctcatggt gagcagcggc cgggtgtcga agccggagtc
     7801 gacgctctgc agctccagga agctgcggat gaacagcgac gctcccacca gcaggagcag
     7861 cgacagggcc acctcgacga tcaccaggcc gttgcgcagg cggttgcgaa ggacgctgcc
     7921 gccggcgccg cgccccccct ccttgagcgt gtcgttgagg tcggtcttgg cggcctggag
     7981 cgccggcgcg aggccgaaga cgagccccgt gaccactgcg attccaaggg tgaagagcag
     8041 gacgttgccg tcgatgacga agcgcatcca gtagggcggc tggttctcct gcgggatcgc
     8101 cgcctccatc aggtggatgc cccagacggc gagcaggatg ccgagggcgc cgcccaccag
     8161 ggccaccacc acgctctcgg tgaggagctg gcggatcacc cggcgccgct gcgcgccgaa
     8221 ggcgacgcgg acggcgatct cccgttgccg cgccgtggcg cgcgccagga gcaggttggc
     8281 gacgttggcg caggcgatga ggagcacgca gatcacggcg cccatcatgg tgagcaccat
     8341 gagctggatc tccgtgtcga cgaagtcctc gcgtagcgtg accgcgcgcg cgctccagcc
     8401 ggcgtggctg tccggatact cccgctccag ccgcttgaag aaaccctcca gctcgacgtt
     8461 ggcctgctcg aaggtggcgc ccggccggag gcgggccatg gcgacgaggc tccggtcgac
     8521 gcgctcgccc ttgtgcagga tcggcgtcgc gggcacccag gcctcctgct ggtaggggaa
     8581 gttgaagtgc ggcgccatca cccccaccac ggtgtgggcc gtggcgttga ccatcaccgt
     8641 ctgcccgacg atcttcgggt cgctgttgaa gcggcgctgc cagagggcgt ggctgagcag
     8701 gatcaccccg ggcgcgcccg gccggtcctc ctcctccagg aacaggcggc catggatcgg
     8761 cttctcgccg agcatctcga acaggttcgc cgacaccagc gcgccgagga cccgctccgg
     8821 ctcgtcgtga cccgagaaca ccaggctgcg tgggccatag gcctcgatct tcgagaagga
     8881 gcggacgctc gcgcgcgcgt cctggaggtc gtgcagggag aagttgtcct cctcgatgcc
     8941 gcgctggagc tgggtctcgt agaggacggc gatccgctcc gggtcggcat agggcagcgg
     9001 ccggagcagg atggcgttga cgacgctgaa gatggtggta ttggcggcaa tgccgagggc
     9061 gatgcagagc acggcgatga gcgtcacccc cgggctcttg agcatgaccc gcagcgagaa
     9121 gcgcagatcc tggatgagat ggtccatggt ccctcccgtt atccctacgg cctgggcggc
     9181 gccctctgcg aggaaatccc tgcttcgtcc ttacgcagag gctacgtgcc gggtttcatc
     9241 tccgggtctc atctccgccg gggcgggtta tcctggccgc ggatgaaacg tctgctggcg
     9301 atctggctca ccctcctcgc cgcgttctgg tttacccggg cggcggtttc ggcgttgctc
     9361 ttccagagcg ccgacctgag cgagcgcgcc gtcttccagc tcgtcaccgt ccccgtcctc
     9421 cagacgctgg tggtgggctg gctgacccgg gaccccgggc gcctgcccct cctcgcaccg
     9481 ctacgcgcgg cgctggccca cccgggagtc cgcgcgatgc tggtgctcga cctgctcctg
     9541 gtgctggcgg gtgccgtctt ttaccgcaac cccggcctgg cgccggcgcc gccaagcctg
     9601 ccggccctct gggtcgggct gaagctcctg gcggcgggcg gcctcctggt gctcctgtcg
     9661 cggcggggcg atgcgggaga gcggctccga ttgtatgtcc tggcggcgtc cctggccatc
     9721 ctgggagtgg tcggcgccac cggcttcctg gactggtccg gggtcctgct cgcgcgctgg
     9781 ctgcccgagg cctcgcccat ggcgctctgg ctattcaccc gcgcccccct cgccgtggcg
     9841 gccgccgccg ccctccttga ggtgcaggag atgtttcgtc cttcggaccc gggcgcggcc
     9901 cgggcgctcg actgggccct ggggttgggg ctcctcgccg cgctcgtcac cgtcctcgac
     9961 ctcttcgccc tgccctaccc cgccgccccc tggggtcccc tcgtcaccgc cagcgccacg
    10021 ctcggctcca cggcgctcct catcgggctg gcgctggcgg gcggctggac cgaagcccgg
    10081 gattgaggga tctaccagcc cggcagcgcc aactgctcgg ccagccagac ggtccggtcg
    10141 tggcggatga tctccgcacc catcgggtga tcggaggcgt aggtggcgag ccgcttggtg
    10201 ccgggcgtgg ccatgtagag ggcgatgaag tcctcacgcg gcgtgtagcg gtccgcctcg
    10261 ccgaattgat agaagacggc ggccctggcc tgggcgatgg cgttgaccgg ctcgagggga
    10321 gcgagccgcg ccttgaagcc cgcgagcgcc tccccctccg gcacgccgct cgccgagccg
    10381 aacagatacc agtccgagaa gcgcgccgtc ccggcgatca tcacgtacgc cttgggacgg
    10441 gggtccaccg cggccaccag ggcgccgaac atcgcgccgt agtcgtggcc gacatacccg
    10501 acacgcccct tgtcgacacc gggctgggcc agcagcacgt cgagcgcgcg gcgcagctcc
    10561 ttcgcctggc cgacggtgtt ctggtagtcg tcctcccacc ggcgtcccct gtaccgggcc
    10621 gggtccgacc agaaggtgga gacgaggagc gacacggtcc cccggcgcgc cagcgcctcg
    10681 gcctcgctca ggtattgagt gcggttcgac gtcgcgaagg acggggcgta ccagtggacg
    10741 aagagcacgc ctgcgaacgg tccggcctcc tcgctcggcc ggacgacgta cgcctgcacc
    10801 ggagcgccac ccgccaggct ccggaaggtg acgtcctgca ccgtgacccc tggccgcttc
    10861 tccgtgccga ccacctggag gtcgagaggt acaccggtgt catgagagag gatctgggac
    10921 aggtcggcgg cgcgggacgc ccgccccatc cccgcgagca gcaaggccgc cagaagggcg
    10981 gcgcgacaga tcgaacgttt catggtcggg cgactccaaa aattgacagc ctctcagcct
    11041 ttctttccct gagtctctgt gaagagccgg tagaactctc cgggattctc cagatacatc
    11101 tccgtgtacc cgcactcgcc acagacccgc gcgaacaacg agccttcatg cgggcccttg
    11161 aagatcatcg cttcgggctt ctcatagaca atcagcgcca gctccccggc atccaagctg
    11221 taatcccccc ggtccatgac ccgcactttc ggcagcactt tcgtggatcg gcacttcgcg
    11281 catgcggctt cggtcatgat cactctactc ctcgataggg tcacggttca gaacgcccag
    11341 gcccagcgcc gtcgccagcc aggcaaggcc catccaggcg aaggcccatt ccacaaggaa
    11401 gcggaggccg gaccagaggc ccaggaggcc ggccgcgaac atcagggcgc cgatcagata
    11461 ggcgagcccg gggacgaagg acaacaccct gaggatccag gccatgcgct cacgctcctt
    11521 ccggggagct cctgcgagtg ttgctgaaaa ggggccgtcc tcaggcctgc agccggcggg
    11581 cgagctgctc ggcgcgctgc tggttggcgc tctccgggtc cgcgggacgg agctcgcgcc
    11641 gcaccgtgga ggccagccgc cgctcccggt ccgcctcgga gagctgccgg tcgttgacca
    11701 cgtcccaggt ctggctgacc acgatggcct cgaggttctg gacgcggtcc aggagctccg
    11761 tctgccgccg ctgcatctcg gccagggcct tctccagctc ccggttgtcc gccccgagct
    11821 tccgctgctc ggacctgagc ttgaggtagt tgttgaacat gcccgcgccg ataccggcga
    11881 gggggatcag cacccagata ttggccatca ggactgccaa ggcgttgtcc aaggataacc
    11941 tcccgggccc gcgactgctc gcggcagggg gcctgaggta ttgtacgtcc cgccgggccc
    12001 ggggttttca gagaacggcc cggaaccgca cccgctctcc tggcagactt gctccgagat
    12061 gcaggcggtt tgccccacgg ggcaagtggc gcgacccgag atgcggacct cttgccccag
    12121 ggggccaacg gcttgcccca gggggcggat ggctcgcccc gaaatgtaga ccgctcgccc
    12181 caaggggcca acgactcgcc ccatggggca gacagctcgc cccacggggc caacggctcg
    12241 ccccaagggg cgaacggctc gaccccaggg gccaaccact ggacccacgg ggcagacggc
    12301 ttgacccgcg gggcagacga cgtcgagatc ggcgcctccc gccaggaacg gggctctcag
    12361 gcccccgccc ggaaccgcac ccactccccc ggcaccggca tggtccagct ccggcaggag
    12421 gggccgcaga cgtccccctc ggggcccacg gtgtcggcct cccagccgct catcgcccgg
    12481 cgggcccggt ggaagtagcg gttggcccag aagtcggtca gcagggtgac gaagcggacg
    12541 ggtttgccgg cggcgtggaa gaagctctcg tagtgggtga ccagcacatg acgcggcctg
    12601 agatggccga gaatggtccc gggctgcttg cgcacgaagt agaacgaggc catgcacagg
    12661 accgccagat cgtacccgcg gcagtcctcc aggcgaggga tgccctgttt cggcgggttc
    12721 gccgagtcct gatagtagag acggaagcgg gtcttcttca gatcctgcga caagagatcg
    12781 atcacgaatg cgaaggtctg cccgtccttg agcgacagca gacggtgcgt ctgccactcc
    12841 cccgtccagt ccgcggcgag cgggccgcgc aggagacgga cgccccagaa gtgcggggcg
    12901 tgcgccgact tcaccgcccg gaagcggatc ggcaggtcgt tctggtcggg atctttcaga
    12961 taaatccaat cccggcccgc ctcgctctcg agggaggtga tccgtccggt cagggcgcgg
    13021 acaggcgcca gggcatgagc gccgttgttg ttgacataga cccgggcgcc cggcgcgaac
    13081 cgctccgcca ccagcggcag gtcgccgatg tgatcgtagt gggagtgccc ggcggcgatg
    13141 gcgcggacgc gcgagagatc catcggtttc aggccgcgct cgaccgccgc ctcgtccgtg
    13201 accagacggc cccagggcac ccgcaagatc cgggggttcg agaagaaggg cgacatcagg
    13261 agggcggtcc cctgccactc gacgtagagg ccgcctgccc cgagatagcg gatcgccacc
    13321 tcgtccggcc ccggctccgg cttcggcgac ggctggcagg ggggcttctt gaaatcgaac
    13381 gccccctggg aggcatagtt gggagcgaac ttcgcgcgga tcgtgtggcc gccgcagccg
    13441 gtcagcgcga gcaggaggat cagcgcccag cttgaccgca ccgccgctcc gcggctagcc
    13501 tgcgggcgag ggataccatg ccgcctctcg aatccaccct gcggacgcac gacggtctgg
    13561 cgctcatcgg cgagcaccac ctgcggccgg gctgccgcgc ccgcgtggtg atcgttcacg
    13621 gctacgccga gcaccggggc cgctacgcct ccctggccgc ggccctgggc gaggcgggct
    13681 acgagtgcca tctcctggat ctgcgcgggc atggcgggtc cgggggggtc cgcggcttcg
    13741 tttcgcggtt cgaggagtac ttcgccgacc tcgacctctt cctgacgcgg atcgaagagg
    13801 tggcggccgg aggcattccc ctgatcctcc tgggccacag ccttggcggc ctgatctccc
    13861 tgggatacgt gctgcagcgt cccggccggt tcgatgccct ggcggtcagc agcccgttcc
    13921 tcgcccccgc catgccggtg ccgccgctcc aggccggact ggcggcggtg acctcccgtc
    13981 tcgcccccaa gctcctcggc ccgagcccca tccggccgga gtcgctgagc cacgacacgg
    14041 aggtggtggc cgcctacgag gccgacccgc tggtgttcaa gaccttgagc ccgcgctggt
    14101 tcatggaggt gcgggaggcg caggaggagg ttctcgaacg ggccggggag atccggctgc
    14161 cggcgctgtt cctgatcggg ggcgccgatc ccatcgccga tccggagcgc tcgcgcagcg
    14221 tcttcgagcg gctgggcagc gccgacaagc atctggagat ctatcaagat ttcctccatg
    14281 aggtcttcaa cgagaccggg cgccagcggg tgatcgacga cctcgtcaac tggctggacc
    14341 gccgggctcc tcccgtctcc cctcgaccgg cacgctgagc tggaaacgcg gccggcccca
    14401 gctctgcacc ataccgatgc ggcggccgat gtcgcgccgc gccaccctct ccatccgctc
    14461 caccgtctgc tgccggaaat cgaacagcgg cggccagtcg ggctcctcgc gcgacggcgc
    14521 cagcgacacc acgaagaggt cgcggcggct caggcggtcc acctgctgcg agcgctcgaa
    14581 gaggaagcct gggaggcggc cgaggttctc caccagcttg ccgcggaacc ccaggagatg
    14641 ggcggcggag cgcgacagcc acgtcggcgg tgactcgtgg ccgagcggat tggtggagtc
    14701 gacgatcagc acctggctcg ccgacacggt cagcgccacg tccaccggca cgttgttcga
    14761 atagccgcca tcgaccagcg gtcgcttctg tccgtcgaac tcgacccggt gggccgggaa
    14821 gatcgggaac ggcgagccgg aggcgaagat cacgtcgcgc aggaagaccg gatcgggcac
    14881 cggctcgcaa tcgtcatcgg aggagctcgc ctcggccggc ttgccgaacg tggcttcgta
    14941 gctgagccac tgcgcgccgc tcgcgggccg ccgcgggatg gacgggcact cgtccccctc
    15001 cggaatgaag aggaaatagc gcgtgccgtc cttctccagc aggttggccg gcgcgatgaa
    15061 gcgggccgtc ggacggtagg ggaccccctg gaggcccgcc ctggcccgga actggtccgc
    15121 cgcgctgctc aggtagcgga acgccggccg gttgccgtag agggccggcg ccaccaccag
    15181 gttccaccag cccaggcagc agaccgacca gacggccatg cgcaggaacc ggcgggtgat
    15241 cacgtcgccg ttggggtgat gggagctgag gaaggcgaag ctccggtgga ggtagccggc
    15301 cacggcgctc cagagtctcc ccccaccaga agggcggccc ggctgccggc cggtcggccg
    15361 cccgccgagc ccggcgagca gcatcaggcc tcccaggagc aggctcgtcg ccagcagcca
    15421 gagccagaac tcgcgggtca gctccagggg ggacagccag tcgggcagca gcgcgatcac
    15481 caccaccagg accaggtaga ccgtcaccgc gtgcaggagg accacgaagt aggggacgag
    15541 gaactcggtc agccgcaggt gatagcggcg ccgggaggcg tacacccagg cgatgccgcc
    15601 gagcatcagg atcagcagcc cgacgccgag caggaaggag cccaccgaca catccagggt
    15661 gcgggcgccc aggctcgccc ccaggctctc ccagctccgg gtgggcgcgg cctgctccgg
    15721 ccagcagagg gtgacgagga gcagcgacga ggcgagaagc gctccccagt agatccgctg
    15781 ccgcgtgcgc tcggcgaggc cggtgcgggc cgccagcagg aagttggcgc cgacgctgac
    15841 caggaccatc aggaacccgg ccaggaagaa aggcatcttg atcgcgtcga gccacgggcg
    15901 cggcaacagg aagagaatgg cgagggcgag cccgccggcg gcggcgaaat gaagcaccgg
    15961 ccgcatccag ccgatcgccg cgcggtcgcc cgcggggcgc cccgtccgca acggcaggat
    16021 gagccctccc aggaggagca gcggggcgag caccgcgaag gccgggccga aggtgagcgg
    16081 ctcgccgagc atgcgtccga tccgcgagcc cccccagggg agcagcggga ggagcacgag
    16141 aaggcccccc agcgccaccg gcagcgcggg gggcacccag ggctcgcccc cctcgcgctc
    16201 gcggtcgaga cggaccatcg cctggtcgac caccatcgcg atcatcgcga ggaccgcgta
    16261 gaccagacct tcgagctcgg ggacgtgctc ctgcagggtg tcgccgctca cccggcgcac
    16321 caggagaggg gcgagggcca gcaccggcag caggacgccc agcatcagcg gccggcggac
    16381 ccccatcccc ctacccaccg gctccgggct gaaaggggag cgctccggga tggaggcaag
    16441 ggcgatgagg ccgcagagca cgagaaagct catgatgacg ctcacgtagc gcagcaggtc
    16501 ggtccagccg aagatgtcgg tgctctcgac gaagctgccg tccgccttct tccagaggat
    16561 gtcggacagc ccccagggcc cgttctcccc gagctgcgag acgaagaacc ccagcaaggc
    16621 gccgccgctc gtgccgatga cgtactggac gtccagcggc tcggcgctgc cgggtgcggg
    16681 ctcgaccttg aagttcttga ggtatccctg gcggtacagt gaatccacca ccgtcgattg
    16741 gtaggccgcc ttgacgccgc ctcccggcag caccagggcc aggtcgctgt ggcccgaggt
    16801 gatctgctgc cggcgcagga ggccgaagat ctcgtcgacg gtgaagctga acgtgcgcac
    16861 gccgaacgtg ggctccggct cgaggagggg gaggagagcg gccaccggcc gttcggcggt
    16921 caagagggcg gcggcctccg gaggaagctc ggcctgggct tcctgcatcc cggattca
//
LOCUS       DASPJI010000269         5439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_249693, whole genome shotgun sequence.
ACCESSION   DASPJI010000269 DASPJI010000000
VERSION     DASPJI010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249693"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="VEL74_07130"
     CDS             <1..1113
                     /locus_tag="VEL74_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62336.1"
                     /translation="DSLVFALAVDPRDADTVYAGLTEGIARSTDGGATWTRLSLETLA
                     TVRALVLDPSNPDVLYAGTPYGIFRSPDRGTTWLVYSQGLQNREVLDILISPPSLPMA
                     QATIYSGTRGGGVYRLDRAPCVPGDNILCLQGGRFQAAVSWRDFQGQTGTGHAVPLTP
                     DTGAFWFFGRDNLELMVKVIDGRAVNQHYWVFFGALTNVEYTLVVTDTETGQTRPYFN
                     RSGLFASRGDTAAFRALDFTNPVSDAAADEAPAGPVTSPAGACAPGDTALCLEGGRFR
                     VEVTWEDFAGNSGAGHAVPLTPDTGAFWFFGPANLELMIKVVDGGAVNGKYWVFYGAL
                     TNVGYTIRVTDTATGAVRTYTNPQGTFASAGDTQAF"
     gene            complement(1114..1293)
                     /locus_tag="VEL74_07135"
     CDS             complement(1114..1293)
                     /locus_tag="VEL74_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62337.1"
                     /translation="MAERKAFPLRIDPEVFEALRAWADDEMRSVNAQIEFLLVRALQQ
                     AGRAPRGKRGTGKKE"
     gene            complement(1316..2185)
                     /locus_tag="VEL74_07140"
     CDS             complement(1316..2185)
                     /locus_tag="VEL74_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008221735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HYG62338.1"
                     /translation="MIRETEFNGRPGFLMLFVWLALLLGFSVGMYYSVRASSAVGLAV
                     MVLGLVASVVGLCGLFMVNPNESKVLQLFGDYKGTAKLAGLRWANPFYSKKNVSVRTR
                     NFETGKLKVNDNSGNPIEIAAVVVWKVVDTAEALFNVDDYVNFVQVQSEAAIRNLATH
                     YAYVADHEGEMALSSHTAEISVGLRDEIQARLEQAGVQVIEARISHLAYAPEIAAAML
                     QRQQASAVVAARAKIVEGAVGMVEHALEMLSEKQIIDLDPERRAAMVSNLLVVLCGDR
                     HTQPVINTGSLYH"
     gene            complement(2350..3111)
                     /locus_tag="VEL74_07145"
     CDS             complement(2350..3111)
                     /locus_tag="VEL74_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62339.1"
                     /translation="MACMLALAVPAMADDVIYNGSDLWQTKGDGKTFADFSREPIPAG
                     FFCAKSEPFTDRIAFKGVPLRSSEGTLPIDTIVQRLDDAGFDNKGVAMTRIQMRALLL
                     ESVAPVKTACGSFHVKVRLNGRQPVTRMRIVRQGEDGGFFVSNLAVNAKLSFHPVSGK
                     ARERLEINQRIRFNPSRTAWAVRSDNNKSVEKKGFLLVDTDGDNRPDTYLPGTSNFAA
                     GWRAVQGKARYDNTDVVIDEGWHENPTHEHTVTGW"
     gene            complement(3247..>5439)
                     /locus_tag="VEL74_07150"
     CDS             complement(3247..>5439)
                     /locus_tag="VEL74_07150"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HYG62340.1"
                     /translation="GRADGLRKEPDGSVVVEEIKSVRRGGTLPPAVREMYQRQAALYA
                     WMLGKTSEAEVKAELVLIAIGSDEMERETVEVNLPALEVSIRRRLNALIYSYESDRQQ
                     AAERRAAAERLSFPYREPRPGQELILAAVETAVANREHLLLQATTGIGKTVAALYPAL
                     KYALANDKRVFVLTAKTLQQDMAAAVLSLLNQEGAFRSLRLRAKAKMCANDQIVCHEE
                     YCRFAKDYALKLVGSGVLGRIWAEYRSIEPDDVFAASRQAEVCPFEVSLELAGKAQVV
                     VCDYNYAFDPYVSLTEFAADNDLSDVVLVIDEVHNLVDRGRGYYSPELSAAAARHAAE
                     ASAHGGAPVHLRIQTLCLQLARLIEDTVSDALEEGPPGDRAVEWPLPEDRFWRLRPHL
                     DEVFVDYLEYQKETHSFRPEDPFVELYFNFLRFLNGLVVSDEAFSHYVEARRGEARLK
                     ILCKDPSRFLGAVISRAHAAIGLSATLSPPDFYTGLLGFEAGRTAFVEIPNPFPLENR
                     RVVIDPSIVTTYKQRPNNYDRIAERLASFADAVPGNCLALFPSYAFLSEVTGRMRLNH
                     KRVLVQRQADSDKERDLILQALRSAVFGDVLLVAVAGGVFAEGVDYPGEVLKAVAIVG
                     PCLPALTLEQELLKAYYDERFERGFEYSFVVPGMTRVVQAAGRLIRSPEDTGVIALFD
                     QRFLVGPYRHHLPPDWLPGDDAGSLVGNPAKVAEEFFRLLAERERVRT"
BASE COUNT          826 a         1830 c         1914 g          869 t
ORIGIN      
        1 gattccctgg tcttcgcgct agccgtggac ccgcgggacg cggacacggt ctacgccggc
       61 ctcaccgagg gcatcgcccg cagcaccgac ggcggtgcta cctggacccg cctgtcgctg
      121 gagaccctcg ccacggtgcg ggcgctggtc ctcgatccgt ccaacccgga cgtcctctac
      181 gccggcaccc cgtacggtat cttccggtcg ccggaccgcg gcaccacctg gctcgtctac
      241 tcccaggggc tgcagaaccg cgaggttctg gacatcctga tctctccgcc gtccctcccg
      301 atggcgcagg ccacgatcta ctccgggacc cgtggcggcg gcgtctaccg tctggaccgc
      361 gccccctgcg tgccggggga taacatcctc tgcctccagg ggggccgttt ccaggcggcg
      421 gtctcctggc gcgacttcca gggacagacc ggcacggggc acgcggtgcc gctcacgccc
      481 gacaccggcg cgttctggtt cttcggccgg gacaacctgg agctgatggt gaaggtgatc
      541 gacggccggg cggtcaacca gcactactgg gtgttcttcg gcgcgctcac caacgtggag
      601 tacacgctgg tcgtcaccga caccgagacg ggccagacca ggccgtactt caaccggtcg
      661 ggcctctttg ccagccgggg cgacaccgcg gccttccggg cgctggactt caccaatccg
      721 gtgtcggacg cggcggccga cgaggccccg gcggggcccg tcacctctcc cgccggagcc
      781 tgcgcgccgg gggacaccgc cctctgcctg gagggcggcc gcttccgggt ggaggtgacg
      841 tgggaggact tcgccggcaa ctccggcgcc gggcacgcgg taccgctcac tcccgacacc
      901 ggcgccttct ggttcttcgg cccggccaac ctggagctga tgatcaaggt ggtcgacggc
      961 ggggcggtca acggcaagta ctgggtcttc tacggcgcgc tgacgaacgt cggctacacc
     1021 atccgggtca cggacaccgc gaccggggcg gtcaggacgt acaccaaccc gcagggcacg
     1081 ttcgccagcg cgggggacac gcaggcgttc tgatcactcc ttcttccccg tcccccgttt
     1141 cccccggggt gcgcgccctg cctgctgcag ggcgcgcacc aggaggaact cgatctgggc
     1201 gttgacgctc cgcatctcgt cgtcggccca ggcccggagg gcctcgaaga cctccgggtc
     1261 gatccgcagc gggaaggctt tccgctccgc caaggcgttg acctcaggac tggcctcagt
     1321 gatagaggct gccggtgttg atcaccggct gggtgtggcg gtcgccacag agcaccacca
     1381 gcaggttgct gaccatggcg gcccggcgct cggggtcgag atcgatgatc tgtttctcgg
     1441 acagcatctc gagcgcgtgc tcgaccatgc cgaccgcgcc ctcgacgatc ttggcgcgcg
     1501 ccgccaccac ggcgctggcc tgctggcgct ggagcatggc ggcggcgatc tccggcgcgt
     1561 aggccaggtg gctgatgcgg gcttcgatca cctgcacacc ggcctgctcg aggcgcgcct
     1621 ggatctcgtc gcgcagcccc accgagatct cggcggtgtg gctggacagc gccatctccc
     1681 cctcgtgatc ggcgacgtag gcgtagtggg tggccaggtt gcggatggcc gcctcgctct
     1741 gcacctggac gaagttgacg tagtcgtcga cgttgaacag ggcctcggcc gtgtccacca
     1801 ccttccacac cacgacggcg gcgatctcga tcgggttgcc gctgttgtcg ttgaccttga
     1861 gcttgccggt ctcgaagttg cgggtacgca ccgacacgtt cttcttgctg tagaacgggt
     1921 tggcccaccg caggcccgcc agcttggccg tccccttgta gtcgccgaag agctgcaaca
     1981 ccttggactc gttggggttg accatgaaca ggccgcacag cccgacgacc gaagccacca
     2041 ggccgaggac catcacggcg agccccaccg ccgagctggc ccggaccgag tagtacatgc
     2101 cgacggaaaa gcccaggagc agcgccagcc atacgaagag catcaggaag cccggcctgc
     2161 cgttgaactc cgtctcacga atcatcggtt tctctccttg tctccccgtc cattggggat
     2221 gtcgaagtga tatcacagtg atgtgtacgg atcaaggccg ggaaggttgc ggaggagccg
     2281 gggagagggg gcggcaggag ggggaggaaa gggggaggga cccggcgggc cgggtccctc
     2341 gggaggggct taccagccgg tgaccgtgtg ctcgtgagtc ggattctcgt gccagccctc
     2401 gtcgatgacc acgtccgtgt tgtcatagcg ggccttgccc tggacagccc gccaaccggc
     2461 ggcgaagttg gaggtgccgg ggaggtaggt gtccggccgg ttgtcgccgt cggtgtcgac
     2521 gaggaggaag cccttcttct cgaccgactt gttgttgtcg cttcggaccg cccaggcggt
     2581 gcggctggga ttgaagcgga tccgctggtt gatctcgagc cgctcgcggg ccttgccgga
     2641 gaccgggtgg aaggagagct tggcgttgac cgccaggttg ctgacgaaga agccgccgtc
     2701 ctcgccctgc cgcacgatgc gcatgcgggt gaccggctgc cggccgttga gccgcacctt
     2761 gacatggaag ctgccgcagg ccgtcttgac cggggcgacg ctctccagca gcagggcgcg
     2821 catctggatc cgcgtcatgg cgacgccctt gttgtcgaag ccggcatcat cgaggcgctg
     2881 gacgatggtg tcgatcggca gggtgccttc ggagctgcgc agcggcacgc ccttgaaggc
     2941 gatgcggtcg gtgaacggct cggacttggc acagaagaag ccggccggga tcggctccct
     3001 ggagaagtcg gcgaaggtct tgccgtcgcc cttggtctgc cagagatcgc tgccgttgta
     3061 gatgacgtcg tcggccatgg cggggaccgc cagggccagc atgcaagcca ttgctaccaa
     3121 catccccttt ttgttcacag aacctccaag tccagaaagg gccgcgcaca ctcgcgcggc
     3181 actggagagg atggcccagg tagggggctg aagacttctt ccagattgct attttcgagg
     3241 ggaggcctac gtccgcaccc gctcgcgctc ggcgaggagc cggaagaact cctcggcgac
     3301 cttggcgggg ttgcccacca gggagccggc gtcgtcgccg ggaagccagt cgggggggag
     3361 gtggtgacgg taggggccga cgaggaaccg ctggtcgaag agggcgatca ccccggtgtc
     3421 ctcgggcgaa cggatcaggc ggccggcggc ctggaccacc cgggtcatgc ccggcaccac
     3481 gaaggagtac tcgaagccgc gctcgaagcg ctcgtcatag taggccttga gcagctcctg
     3541 ctccagggtc agcgccggca ggcaggggcc gacgatcgcc accgccttga gcacctcgcc
     3601 ggggtagtcc accccctcgg cgaagacgcc tccggccacc gccaccagca ggacgtcgcc
     3661 gaacaccgcc gagcgcagcg cctggaggat caggtcccgc tccttgtcgc tgtccgcctg
     3721 gcgctgcacc agcacccgct tgtgattcag gcgcatccgc cccgtcacct cggagaggaa
     3781 ggcatagctc gggaagagcg ccaggcagtt gcccggcacc gcgtcggcga agctcgccag
     3841 gcgctcggcg atccggtcgt agttgttcgg ccgctgcttg taggtggtga cgatggacgg
     3901 gtcgatcacc acccgccggt tctccagcgg gaacgggttg gggatctcga cgaaggccgt
     3961 ccgccccgcc tcgaagccga ggaggccggt gtagaaatcg gggggagaga gggtcgccga
     4021 caggccgatg gcggcgtggg cgcgcgagat cacggcgccc aggaagcggc tcgggtcctt
     4081 gcagaggatc ttgaggcgcg cctccccccg ccgcgcctcc acgtaatggc tgaacgcctc
     4141 gtccgacacc accaggccgt tcaggaagcg gaggaaattg aagtacagct cgacgaacgg
     4201 gtcctcggga cggaagctgt gcgtctcctt ctggtactcc aggtagtcga cgaagacctc
     4261 gtccaggtgg ggtcgcaggc gccagaagcg gtcctcgggc agcggccact ccaccgcccg
     4321 gtcccccggc ggcccctctt ccagggcgtc ggacaccgtg tcctcgatca gccgggcgag
     4381 ctgcaggcag agcgtctgga tgcgcaggtg gaccggcgcg ccgccgtgcg ccgaggcctc
     4441 ggcggcgtgg cgggcggcgg cggcggagag ctccgggctg tagtagcccc gcccgcggtc
     4501 caccaggttg tggacctcgt cgatgaccag gacgacgtcc gacaggtcgt tgtcggcggc
     4561 gaactcggtc agcgacacat aggggtcgaa ggcgtagttg tagtcgcaga ccaccacctg
     4621 ggccttgccg gccagctcca ggctcacctc gaaagggcag acctcggcct gccgcgaggc
     4681 ggcgaagacg tcgtccggct cgatcgaccg gtactcggcc cagatccggc ccaggacccc
     4741 cgagcccacg agcttcagcg cgtagtcctt ggcgaagcgg cagtactcct cgtggcagac
     4801 gatctggtcg ttggcgcaca tcttggcctt ggcgcgcagg cgcagcgacc ggaacgcacc
     4861 ctcctggttg aggaggctca gcaccgcggc ggccatgtcc tgttgcaggg tcttcgcggt
     4921 gaggacgaag acgcgcttgt cgttggccag ggcgtacttc aaggcggggt agagggccgc
     4981 cacggtcttg ccgatgccgg tggtcgcctg gaggaggagg tgctcgcggt tcgccacggc
     5041 ggtctcgacg gctgcgagga tcagctcctg gccgggacgg ggctcgcggt aggggaagct
     5101 gaggcgctcg gccgcggccc ggcgctcggc ggcctgctgc cggtcggact cgtaggagta
     5161 gatgagggcg ttgaggcgcc ggcggatgga cacctcgagc gcggggaggt tgacctccac
     5221 cgtctcgcgc tccatctcgt cgctgccgat ggcgatgagc accagttcgg ctttgacctc
     5281 ggcctcggag gtcttgccca gcatccaggc atagagcgcc gcctggcgct ggtacatctc
     5341 ccgcaccgcc ggcggcagcg tgccgccgcg gcgcaccgac ttgatctcct ccaccaccac
     5401 cgagccgtcg ggctccttgc gcaggccgtc ggcccgccc
//
LOCUS       DASPJI010000270        12028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_309986, whole genome shotgun sequence.
ACCESSION   DASPJI010000270 DASPJI010000000
VERSION     DASPJI010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12028)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12028
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309986"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /locus_tag="VEL74_07155"
     CDS             complement(<1..1575)
                     /locus_tag="VEL74_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYG62341.1"
                     /translation="MASQDDEDRRLRIAALQNANAILLARQRLERELAAEHERLRITL
                     ASIGDAVISTDSECRVTYLNAVAEALTGWSQAEAAGQPLAEIFRIVNEETREPAENPA
                     LRALKEGVVVGLANHTVLIARDGTERPIDDSAAPMLDELGVPVGVVLVFRDVTERRRA
                     EEAQARLAAIVETSHDAIISKTLDSIIRTWNAAAERLFGYSAEEAVGRSITLIIPPDR
                     LDEEQAILARLGRGERIEHYETVRMAKDGRLLDISLTISPLRDGYGRIIGASKIARDV
                     TERRRAEEALRASELRFRTLAAHAPVGIFLTDPKGNYLFVNQCWCEMAGLSPEEAHGE
                     GWVLALHPDDKDRVTQEWYAAAGAGLPFASEYRFRTPAGKVTWLEVSAVGHYGEEGQI
                     REYIGTVTDITARRAAVEALQEADRRKDEFLALLAHELRNPLAPLRNGLQVIRLASGD
                     SQAVAKARDIMDRQLSHLVRLVDDLLDVSRISRGKVELRLSRVLLSEVVGSAVEIVRP
                     ALKSAGHELTVSLPPEP"
     gene            complement(1575..2636)
                     /locus_tag="VEL74_07160"
     CDS             complement(1575..2636)
                     /locus_tag="VEL74_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003963629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HYG62342.1"
                     /translation="MSIPTEPIGSIPRPLRLIEAVTAMDGISPALDPLYEEAIRDTIE
                     RFEETGSPVITDGEQRKYHNFWTYCVHGMPNTDAEGFKIPFVAGHTRRMPRLTAGPFR
                     YQRYADSYLEVAQRYAHRPVKQAVISPSALSLMYPDDEIPGYPREQFIEDLLREHETE
                     VRNCLRKGAHAVQIDFTEGRLAVKIDPSGHLLHSFIDLNNLALLRFSTEDRRRIGVHT
                     CPGGDLDSTHSADVDYAELLPSLFELMVGNFYVALAGERDRVRVLKIIRECMKSGQRI
                     FVGVISPIDPWVETPEGVRDRVLEAAEYIPLEQLGTTDDCGFAPFCDDASTTRDAAFA
                     KIRARVLGTVLAAEELGVA"
     gene            complement(3345..3845)
                     /locus_tag="VEL74_07165"
     CDS             complement(3345..3845)
                     /locus_tag="VEL74_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62343.1"
                     /translation="MSLPTNAFTAQFLSRLGERDEPPTAGEAAVAGPWRIEPIPGRGF
                     GLFRVGQSLARGHRPAAVCPSRWAALVTAAVLPGTGREPLLRLSKEPEADGLYPVALD
                     TGEVVMRLEYFDETLVDHANALIHVLRSPSSLADALEAGGAEALGLCGSLLDERFPPS
                     EDLPSR"
     gene            complement(4107..5804)
                     /locus_tag="VEL74_07170"
     CDS             complement(4107..5804)
                     /locus_tag="VEL74_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62344.1"
                     /translation="MSPEGDWLLLQSVGVRLFRGGEEQAVAPPTGLAASGACLQQGAP
                     VLAGLTLDLIVGKEDHWKNAPWLLRWEGDRWEPLVSSPVGDGKPKSFGRALEERNLQL
                     FCASDGRVWAAYDQQHHFRQYTAGGRLLREVVVSGGGLREAPNAEALRAIYQKEARVL
                     AEATGGKVIAGEITSQRVAKAITEASDGRMYFLIEGGARGEGPEMALERFDPATLELA
                     RLPLGVPSPGAVTMAAAQDGIYIAAYKASSGIWLFPWQDLDQAEWIPIDDVAVSVEAA
                     DLDGAWREKGGRGLVRFDKDKVITWEDGRLTVRGLVSLEGTGAVLRRAGFGESWKIAR
                     KQATLRIERDGTLREYTRLAEIPKAVSLEPAPLAKAKELAPERVAAIRADLDQRVRRA
                     AEARKKTDLNEWRLAVADNLRYLRELTAENGWIDVRRFGVKAASQAATLAQDGSDVLL
                     MLAALPFLEKDLKPVPEGAAVYSGFYDALQVALGGKQRYGTQIVPDGAGEPVVLPIES
                     RAQVDPVRKKIGLEPLAEFLALASRSLFQGKTIRIAEGEVVEAPKAPAQVGAAAISP"
     gene            complement(6441..7811)
                     /locus_tag="VEL74_07175"
     CDS             complement(6441..7811)
                     /locus_tag="VEL74_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG62345.1"
                     /translation="MSEDEAKQRGAMAVRLIRFIPGWGQETLAKEMGIDPKEVWAWEN
                     FWYAPSRPNLQRAAAAVGVPLGLIEILVPILLWLQRRARGRRRVPAWRIKDEQRRPVG
                     TAWDGVSAEVIWMARELPPLHRWDSPEEDDAARSEIFVERLCEDSERVAADDPARALG
                     LARAAWLMARHARPEDRPRLEGHAGAFEANALRVGSDLDGAEAAFGKARALRLAGAAG
                     TGRLDPSRPLDLEASLRRAQGRFPEALKLLDEALAICRSPEAQGRILLKKGSVLEQMG
                     DSPKAINALRQAARLIDEEREPRQMFAAQFNLGVNLCHLGKFGDASELVNPLRLLAAE
                     LESRSDRVRVRWLESRILAGQGDLENAVITLDRVRSEFEDLGYRYDEAIATLELARLY
                     LRQGRTDKTQELAARAEPVFRELNVAPRVLESVLVFLEAARQEAATLELLDQALQALG
                     EGRRGR"
     gene            7955..8263
                     /locus_tag="VEL74_07180"
     CDS             7955..8263
                     /locus_tag="VEL74_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62346.1"
                     /translation="MFRRLVSLAFILALVFAYVVPGTAMAQPWKTEVFTSAAEGFPSP
                     ADLLAQGWTLLTRLWDKEGCLIDPSGLPAPSSSTQTTPPAQGEEGCLIDPSGLRLNCP
                     "
     gene            complement(8306..9724)
                     /locus_tag="VEL74_07185"
     CDS             complement(8306..9724)
                     /locus_tag="VEL74_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYG62347.1"
                     /translation="MTLKLCREVEDVTQDDVARAAGLTRDEVYNLEKGRTRLQPGQTA
                     YLARILRFPMVVMEGLLHVVRLFYPPPKETSSPPLGLSMKERRIIAGAALTYAATQAE
                     KAAAARLDDHIRAARNQARWVWLEIRTWSVSDCRELLEEAPSLATWALVERLAEESEQ
                     AASADARRALDLAELACWVAGRLEGWKAWRDRVEGFARAFLANALRVSGELKTSEAAF
                     THAWTLWRSGVEAGPGPLDESRLYDLEASLRRALRQLPDALRLLERALDLNPGLESEV
                     RIFIKQAPILELMGRGEDAVAILRRAELLVDEAKNPDQMFALLFNRTVALCRLRRYNE
                     AGAMLGDLGERLMRLGNESQVVRLLWLRSRVYAGLGENKEAETAILQVRRDLMAQGHL
                     YDRALADLELGRLYLRQGRTAETRELASAAEPVFRRLGVEREAEEAVEIFWEAAHREG
                     ANMEMVERALEALLQAKPFPLA"
     gene            10017..10334
                     /locus_tag="VEL74_07190"
     CDS             10017..10334
                     /locus_tag="VEL74_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62348.1"
                     /translation="MNVPPRPKPPSYDPCLWLGSDSHPFHALAQCAADLYLARLRIEK
                     LATDLESHEFYTSRFEDHEATDPIAEMRRAIRCVVQDRLTPAIQELYDFILHPLDGPG
                     EAP"
     gene            10331..11644
                     /locus_tag="VEL74_07195"
     CDS             10331..11644
                     /locus_tag="VEL74_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62349.1"
                     /translation="MSHDAAGRMTWQLVAALGEEHGEDLLHLLTCPECRESAAAVLRA
                     GDGLAGALVPDYDPVFRRLEAGNVFLLEEARRRRGEAGRLVAELRTVPPKQRKKVAEQ
                     QRFERQDVQELLLLESEAVLEDDPQEAVSLGRLADSLARRLSWEDSAQGAVARARAQV
                     LTAGAYRLAGDFSRADTLLRLAIPYLMNASVRGAYCRTLALLRWEEGNVEEAIALLQH
                     GIEVLGEEELAEEAALCAVPLGLLYLEQGATGRALELLLPMKRLDPFARRGLTARAAL
                     GMAQALAELQMPADARVALQGAYRLYDHIRQPLDKVRLYWNESRVLARLGETEQALVL
                     VEQVLAKLLAEKLVPEAALAAVEMTGLLVSLGRNGEVPDRLADLRELCARDWQLRRAS
                     STLNRLAAAVEAGDPALPQRAQRYAQALRQTLRLSYHRVEPPPAA"
BASE COUNT         1990 a         4200 c         3803 g         2035 t
ORIGIN      
        1 cggctccggc ggcaacgaga ccgtcagctc gtgaccggcc gacttgagcg ctgggcggac
       61 gatctccacc gcgctgccga ccacctcgct cagcagcacc cgggagaggc gtagctccac
      121 cttgccgcgg ctgatgcggg agacgtcgag caggtcatcg accaatcgca ccaggtggga
      181 gagctgccgg tccataatgt ctcgcgcctt ggcaacggcc tgtgaatcgc cggacgccag
      241 ccgtatcacc tggaggccgt ttcgcagcgg cgccagggga tttctcaact cgtgagccag
      301 cagcgcaagg aactcgtcct tgcgccggtc ggcctcctgg agcgcctcca cggctgccct
      361 tcgcgccgtg atgtccgtca cggtaccgat gtactcccgg atctgcccct cctcgccgta
      421 atgcccgaca gcgcttactt cgagccacgt cacctttcct gcaggcgtac ggaagcggta
      481 ctcactggcg aacggcagtc ccgccccggc cgcggcgtac cactcttggg tgacacgatc
      541 cttatcatcg gggtgcaagg caagaaccca gccttcaccg tgcgcttctt cgggagacag
      601 gccggccatc tcgcaccagc attggttcac gaacagatag ttgcccttcg gatcggtcaa
      661 gaaaatgcct acaggagcgt gggccgcaag cgtgcggaag cgcagctcgc tcgctcgcag
      721 ggcctcctcg gccctccgac gctccgtcac gtcgcgggct atcttcgacg ctccgatgat
      781 ccggccgtag ccgtcacgga ggggagaaat ggtcagcgag atgtcgagca agcggccgtc
      841 tttggccatc cggaccgtct cgtaatgctc gatccgctct ccgcgtccca atcgagccag
      901 gatcgcctgc tcctcgtcca gccggtccgg cggaatgatc agtgtgatcg accggccgac
      961 ggcctcctcg gcgctgtacc cgaagagccg ctcggccgcg gcgttccacg tccggatgat
     1021 gctatcgaga gtcttgctga tgatcgcgtc gtgggatgtc tcgacgatgg cggcgagccg
     1081 cgcctgggcc tcctcggccc gccggcgctc ggtcacgtcc cggaacacca gcaccactcc
     1141 cacggggacg cccaactcgt ccaacatggg ggcggcgctg tcgtcgatgg gccgctcggt
     1201 cccgtccctg gcgatcagga ccgtatgatt cgcgagcccg acgaccaccc cctccttcaa
     1261 tgcgcggagt gcggggttct cggccggctc ccgggtctct tcgttgacga tgcgaaagat
     1321 ctccgccagt ggctgccccg cggcctctgc ctggctccac ccggtcagcg cttccgccac
     1381 cgcattcaga tatgtgaccc ggcactcgga atccgtgctg atcaccgcgt caccgatgct
     1441 ggcgagcgtg atccgcagcc gctcatgctc ggcggcgagc tcccgctcga gccgctggcg
     1501 ggcaaggaga atggcgttgg cgttctggag cgcggcgatc cggagccgac ggtcctcgtc
     1561 gtcctgggat gccatcacgc gaccccgagc tcttcggccg ccagcacggt gccgagcacc
     1621 cgcgcacgga tcttcgcgaa ggccgcgtcg cgggtggtgg acgcgtcgtc acagaagggc
     1681 gcgaacccgc aatcgtccgt ggtgccgagt tgctcgagcg ggatgtattc ggccgcttcg
     1741 agcacccggt ctcggacccc ttccggtgtc tcgacccacg gatcaatcgg tgagatgact
     1801 ccgacaaaga tccgttgacc ggacttcatg cactcgcgga tgatcttcag gacgcggacg
     1861 cggtccctct cgcctgccag agcgacgtag aagttcccca ccatgagctc gaacaggctg
     1921 ggcaacagct cggcgtaatc cacgtccgca ctatgagtag aatcaagatc accacccgga
     1981 caggtgtgca cgccaatgcg ccggcggtcc tcggtggaga agcgtaacag cgccaggttg
     2041 ttcaggtcga tgaagctgtg gaggagatgg ccagaggggt cgatcttgac cgccagccgc
     2101 ccttcggtga aatcgatctg aaccgcgtgc gcgcctttcc gcaggcagtt gcggacctcg
     2161 gtttcgtgct cgcgtaagag atcttcgatg aactgttctc gggggtatcc aggaatctca
     2221 tcgtccgggt acatgaggct cagggcggac ggagagatga ccgcctgctt gaccggcctg
     2281 tgggcgtagc gctgggccac ttccaggtag ctgtccgcgt acctctggta gcggaaggga
     2341 ccggccgtca accggggcat ccggcgggtg tggccggcca cgaacgggat cttgaacccc
     2401 tccgcgtccg tgttcggcat cccatggacg caataggtcc agaaattgtg atacttcctc
     2461 tgttcgccat cagtgatcac cggagagcct gtctcctcga acctctcgat ggtgtcccgg
     2521 atcgcctcct catagagggg gtccagcgcc ggacttatgc cgtccatggc cgtgaccgcc
     2581 tcgatcaacc gcagcggcct tgggatgctg ccgatcggtt ctgtcgggat actcatgcgt
     2641 cacccccgaa tccgtcctac cccgcccacc gctagtgccg ctgcatccta tgtccctcac
     2701 cccggggtgt caaaccacct ccaggaccgg aggcggaggc cttgggacct ccgcctccat
     2761 tttcctcctc ctggatggag ggttcctcag gggacgaagc ctcgggcacg gcggcgtccc
     2821 ccatcgcatt cagttgtcaa ggagcagtcc tgccgctccg aaccgttacg gcctggttgg
     2881 gagctcatct ctgtatacgg atgcggcgcc tggaacgttc gaatttagaa gaaggaaagg
     2941 cgggaggggc tccctgcaat gatacaaagc ccctggactt cgtatggatt tcccggggct
     3001 tcgatccgaa gccccccacc tcgcgacaaa gtcccgggat tttcagtgag agctccgggg
     3061 cttcgatccg gaggctcggg gcttcgagat gaggccccgg gtgtttcagt gaaagtcccg
     3121 gggctttgaa acgatctccc ggggctttga tgggaagccc ccggactttg ggtccaagcc
     3181 cgggagcttc agcgacaacc ccggtacttc gaagcaaggt gcaggggctt cagaacagag
     3241 ccccggggct gagagacatc tccccggggc tttgacatgt tgtacagggg caccgtgaca
     3301 ggctaccggg accgcggagc ctgccacggc tttccggact cggcctaccg ggacggcagg
     3361 tcttcggacg gggggaatct ctcgtcgagg aggctcccgc aaaggccgag agcttcagcg
     3421 cctccggctt ccagggcatc cgcgagagac gacggggagc gcagcacgtg aatgagggca
     3481 ttcgcgtgat ccaccagggt ctcgtcgaag tactccagac gcatcacgac ctcgcccgta
     3541 tccagcgcga ccggatacag gccgtccgcc tcgggctcct tggacagccg gagcaggggc
     3601 tcgcgccccg tgccggggag gaccgccgcg gtcaccagcg ccgcccagcg cgacgggcaa
     3661 accgccgccg gccggtggcc ccgagcgagg ctctggccga cccggaagag gccgaagccc
     3721 ctgccgggaa tcggctcgat tcgccacggg cccgccaccg cggcctcgcc ggccgtcggc
     3781 ggctcgtccc tctctcccag acgggaaagg aactgagcgg tgaacgcgtt ggtgggaaga
     3841 gacacttcgg acgagctgct accatgggtg caaccacaca taaggacgaa cctccttgtg
     3901 tttcgtggcc aggtccggtc gtgagttacc agctcacttc cggaccgact cggctcatgc
     3961 cgggccacgt cctgccttgt agacgtttga aacccacgcc gggaagcttt cgcggggccg
     4021 cgggccctcc ggcgtgtcct actacgacct caatgcctcc agcatagaag agggcaaagc
     4081 ggaaagcaag cgtctccgaa gagattttac ggggagatcg ccgctgcccc cacctgcgcc
     4141 ggtgccttgg gtgcctccac cacctccccc tccgcgatcc ggatggtctt cccctggaag
     4201 agcgagcggc tggcgagggc caggaattcg gcgagcggtt cgagcccgat cttcttgcgg
     4261 acggggtcca cctgggcgcg gctctcgatg ggcagcacca ccggctcccc cgccccgtcc
     4321 gggacgatct gcgtaccgta gcgctgcttt ccgccgaggg cgacctggag ggcgtcgtag
     4381 aagccggagt agaccgcggc cccttccggc accggcttga ggtccttctc caggaaagga
     4441 agagccgcga gcatcagcag gacgtccgag ccatcctggg ccagggtggc cgcctgggag
     4501 gcggccttca ccccgaagcg gcggacgtcg atccagccgt tctcggccgt cagttcccga
     4561 aggtagcgga ggttatcggc cacggcgagg cgccactcgt tcaggtcggt cttcttgcgg
     4621 gcctccgcgg cccggcgcac ccgctggtcc agatccgccc ggatcgccgc gacccgctcc
     4681 ggcgccagct ccttggcctt ggccagcggg gccggctcga ggctcaccgc cttggggatc
     4741 tccgcgagcc gggtgtactc gcggagggtg ccgtcccgtt cgatgcggag cgtggcctgc
     4801 ttgcgggcga tcttccagct ctcgccgaag ccggcgcggc ggaggacggc gccagtccct
     4861 tccaggctca ccagtccgcg aacggtgaga cggccatcct cccaggtgat cactttgtcc
     4921 ttgtcgaagc ggaccagccc ccgtcccccc ttctcgcgcc aagcgccgtc gaggtcagcg
     4981 gcctccacac tcaccgcgac atcatcaatg gggatccact ccgcctgatc gaggtcctgc
     5041 caggggaaca gccagattcc cgagctggcc ttgtaggcgg cgatgtagat gccgtcctgg
     5101 gcggcggcca tggtgacggc tccgggggac gggactccga gcggcaacct ggccagctcc
     5161 agcgttgccg gatcgaagcg ctctagcgcc atctcgggtc cttctcctct ggctccccct
     5221 tcaatcaaga agtacatacg accgtcggac gcctcagtga tcgccttggc caccctctga
     5281 gaggtgatct caccggcaat gactttgcct ccggtagctt cggcgagaac ccgcgcctct
     5341 ttctgataaa tcgctcggag ggcctccgcg ttgggagcct cccggaggcc tccgccgctg
     5401 accacgacct cccgcagcag acggccgccg gcggtgtact ggcggaaatg gtgctgctga
     5461 tcgtaggcgg cccaaacgcg cccatctgag gcgcagaaca gttggagatt ccgctcctcg
     5521 agggccctgc cgaacgactt cggcttgccg tcgcccaccg ggctcgagac cagaggctcc
     5581 cagcggtctc cttcccagcg cagcagccaa ggggcatttt tccaatggtc ttccttcccc
     5641 acgatgaggt ccagggtaag acccgcgagg acgggggccc cctgctggag acaggcgccg
     5701 gaagcagcca acccagttgg aggtgcgacc gcctgctcct cgccgccacg gaacagacgg
     5761 acgcccacgc tctggaggag cagccagtct ccctcgggac tcatcgtagc ggtgtgatac
     5821 gagccgatcc ctcctccgcc gccggtggtt tcgagttgac gaggcgctcc aagctctccc
     5881 tgggccgata gggggaagac ctcaagggta tccgttcgga gaaggaacgg ctggcccgtc
     5941 ttgtctgcct gcaggaattg ggggcctgtc caggaggctc cagggacgaa ccggcgagcc
     6001 tgcaagctct tgggcagttc ctccgcctgg ctgggccccc acgccaccat ggccaagagg
     6061 accggaagaa tcgactcgaa cctggagcgc ctcggtctca tcacggagca gctctagcaa
     6121 tacctgatga aataacggcg aacctctccc gtactggtgg tgatctcaca gtacgatgcg
     6181 cacgggccgg cgggttcgca gtaaccatca gcctctataa gggccggcaa gaccagagcg
     6241 acgagaacga acagacagcg aaggacgacg cgcttcgaca tacaccctcc tttgttcaga
     6301 gcgctcgtag gtcgggcgct cgatgtcacg ccactcggat cacaagcggt tcaagagtac
     6361 tccccttcca gagtataggc gaattctgga ccactctgac gcttcggcct tctacccttc
     6421 ggtacggacc gcacactccc ctaccgcccc cgcctcccct cccccagcgc ctggagggcc
     6481 tggtcgagca gctccagggt ggcggcttcc tggcgggcgg cttccaggaa gaccaggacg
     6541 gactccagca cccggggggc gacgttcagc tcgcggaaga cgggctcggc ccgggcggcc
     6601 agctcctggg tcttgtcggt gcggccctgg cggaggtaga ggcgcgccaa ctccaaggtg
     6661 gcgatggctt cgtcgtagcg gtagcccaga tcctcgaact cactccgaac cctatcgaga
     6721 gtgatgacgg cgttctccag gtccccttga ccggcgagga tgcgggactc caaccagcgc
     6781 actctcacgc ggtcggaccg gctctccaac tcggccgcca gcagccggag gggattcacc
     6841 aactcgcttg cgtccccgaa cttcccaaga tggcacaggt tcaccccgag gttgaactgg
     6901 gccgcgaaca tctggcgcgg ctccctttcc tcgtcgatga gccgggccgc ctgccgcaag
     6961 gcattgatcg ctttgggcga atcccccatc tgctccaaga cggagccctt cttcagcaga
     7021 attcgcccct gggcctccgg ggagcggcat attgcaaggg cctcgtccag cagctttaag
     7081 gcttcgggga accggccctg ggcgcgtcgg agcgacgcct ccaggtccag aggacgggac
     7141 gggtcgagcc gccccgtgcc cgccgccccg gccaggcgga gagctcgcgc cttgccaaac
     7201 gccgcctcgg ccccatcaag atcgctgccc acccggagtg cgttggcctc gaacgccccg
     7261 gcgtgcccct cgagtcgggg gcggtcctcg ggcctggcat gccgggccat aagccaggcc
     7321 gcccgggcaa gtcctagtgc gcgagccggg tcgtccgccg cgacgcgctc actatcttca
     7381 cagagccgct ccacgaaaat ctcgctacgg gcggcatcat cctcttccgg actgtcccac
     7441 ctatgcaggg gaggcagctc gcgcgccatc cagatgacct ccgccgagac tccatcccag
     7501 gcggtgccca ccggccgtcg ctgttcgtcc ttgatccgcc aagccggaac tcggcgccga
     7561 ccacgggcgc gtcgttggag ccagagcagg atgggcacga ggatctcgat aaggcccaga
     7621 ggaaccccta ccgctgccgc ggcccgctga aggttcggcc ggctaggggc ataccagaag
     7681 ttttcccagg cccagacctc cttgggatcg atccccatct ccttggcgag cgtctcctga
     7741 ccccaaccgg gtatgaaccg gatcagccgc accgccatcg cgccgcgttg cttcgcctcg
     7801 tcctcgctca tccgccactc ctcctctttt ctccccggca cttaccgggg atggcgtacg
     7861 catttccgta gcaaccttgc ggcaccatac tcgccagggc cgcccgagcg tccatggggg
     7921 ccactccggg acggaccccg acaaggagag ccgcatgttt cgacgcctcg tttccctcgc
     7981 cttcatccta gctcttgtct tcgcctacgt cgtccccgga acggctatgg cgcagccgtg
     8041 gaaaaccgaa gtcttcacct ccgctgcgga aggctttccg tcacctgccg atctcttggc
     8101 ccagggctgg actctcctga cacggctgtg ggataaggaa ggctgcttga ttgatcccag
     8161 cggcctccct gcaccaagtt cgtccactca gacgacaccg ccagcgcaag gtgaggaggg
     8221 ctgcctgatc gatccaagtg ggcttcgcct gaactgcccc tgagtagtcc cgccgggcgg
     8281 cagtctcctg ccgcccggtg gcctttcagg ccagtgggaa cggtttggct tgtaagagag
     8341 cctcaagggc tcgctccacc atttccatgt ttgcgccttc ccggtgggcg gcctcccaga
     8401 agatctccac tgcctcctcg gcttcgcgtt cgacccccaa gcgacgaaag accggctccg
     8461 cggcgctcgc cagttcccga gtctcggcgg tccggccctg ccgcaggtat agccggccga
     8521 gttccaaatc cgcaagagcg cggtcgtaga ggtgaccctg cgccattagg tctctccgga
     8581 cctggaggat ggcggtctcc gcctctttgt tctctcccaa gccggcgtac acacgcgagc
     8641 gcaaccagag cagccgtacg acttgagact cgttaccaag acgcatgaga cgctctccca
     8701 gatcacctag catggctccg gcctcgttat aacgacgtag gcgacaaagt gccactgtac
     8761 ggttgaagag caacgcgaac atctggtccg ggttcttggc ctcgtccacc agcaactcgg
     8821 cccgccgcaa gatcgcaacg gcgtcctcgc cgcggcccat cagctccagg atgggagcct
     8881 gcttgataaa aatacgcacc tcgctctcca aacctgggtt gaggtcaaga gcacgctcta
     8941 gaagcctgag cgcgtctggc agctgacgca gggcgcgccg tagtgaggcc tccaggtcgt
     9001 agaggcgaga ctcgtcgaga gggccggggc cagcctcgac cccagagcgc cagagagtcc
     9061 aggcatgggt gaaggccgct tcagaagtct tcagctcacc cgagacccga agggcgttgg
     9121 cgaggaacgc ccgtgcaaat ccctccacgc gatcgcgcca agccttccat ccctccaggc
     9181 gcccagcgac ccagcacgcc agctccgcga gatccagggc ccgccgagca tccgccgagg
     9241 ccgcctgctc ggactcttcg gcgagccgct cgaccagggc ccaggtggcc agcgatggtg
     9301 cctcctcgag cagctcccgg caatcggaga cggaccaggt ccggatctcc agccagaccc
     9361 accgtgcctg gtttcgggcg gcccggatat ggtcgtccag ccgggccgcg gctgccttct
     9421 cggcctgggt ggcagcgtag gtgagggcgg cgcccgcgat gatccgccgc tccttcatgg
     9481 agagaccgag cggcggcgac gaggtctcct tgggaggcgg atagaacagg cggaccacgt
     9541 gcagcagccc ctccatgacc accatcggga aacggaggat ccgcgccagg tacgcggtct
     9601 gccccggctg gagacgggta cgccccttct ccaggttgta gacctcgtcg cgggtgagtc
     9661 cggcggcccg cgccacgtcg tcctgggtca cgtcctcgac ttcgcggcag agcttgagcg
     9721 tcaatccttc cggcggcgtt ttcggcatga accctcctcg tctccttcag ttgccgcagg
     9781 acgtccctga aaggacatcg tccgcgaaga aacttgcgcg ctccacgggt cctctggcgc
     9841 tcgtgcgtaa aactgtacgc catccccggt gcaccccggg gatggaagat aggcaggctc
     9901 aaaccgccac ggcttgaggc atcctctccc cgtcagctcc gccgactacc gcgaagtacc
     9961 gttttgaggc gggtcgtccg gaacccggcg gggctgacgc tcgaaagtac gacatcatga
    10021 acgtgccacc gcgccccaag ccgccttcct atgacccctg cctctggctg ggctccgatt
    10081 cccatccttt ccatgccctg gcgcaatgcg ccgccgacct ctacctggcc cggcttcgca
    10141 tcgagaagct cgccacggac ctcgaatcgc acgagtttta tacctcccgc ttcgaagacc
    10201 acgaagccac cgatccgatc gctgagatgc gccgggccat ccgctgtgtg gtgcaggacc
    10261 gcctcacgcc ggcgatccag gagctctacg acttcatcct ccatccgctc gacgggcccg
    10321 gggaggcgcc gtgagccacg atgccgcggg ccgcatgacc tggcagctcg tcgccgccct
    10381 gggagaggag cacggggagg acctccttca tctattgacc tgcccggagt gccgggagtc
    10441 ggccgccgcg gtgctgcgcg ccggcgacgg cctggccgga gccctggtgc cggactatga
    10501 cccggtgttc cggcggctcg aagcgggcaa cgtcttcctt ttggaggagg cgagaaggcg
    10561 gcgcggcgag gcgggccgcc tggtggccga gttgcggacc gtgccgccga agcagcgcaa
    10621 gaaggtggcc gagcagcagc gcttcgagcg gcaagacgtt caggagctgc tgctgctgga
    10681 gagcgaggcg gtgctggagg acgacccgca ggaggcggtc tccctcggac ggctcgcgga
    10741 ctccctcgcc cggcgcctct cctgggagga ctcggcccag ggcgccgtgg cgcgggcccg
    10801 cgcccaggtg ctgaccgccg gcgcctatcg gctggccggc gacttcagcc gggccgatac
    10861 gctgctgcgc ctcgccatcc cctaccttat gaatgcctcg gtccgcggcg cctactgccg
    10921 aaccctggcg ctgctgcggt gggaagaggg caacgtcgaa gaggccatcg cccttcttca
    10981 gcacgggatc gaggttctgg gcgaggagga gctcgcggag gaggcggcgc tgtgcgccgt
    11041 gcccctcggt ctcctctatc tggagcaggg ggccacggga cgtgctttgg agctgctgct
    11101 gccgatgaag aggctcgatc cattcgcgcg gcgcggcctg accgcccgcg ccgctctcgg
    11161 catggcccag gcgctggccg agctccagat gccggccgac gcccgcgtcg ccctccaggg
    11221 cgcctaccgg ctctacgacc acatccgcca gcccctggac aaggtgcggc tgtactggaa
    11281 cgagagccgc gtgctggccc gcctgggaga gaccgagcag gcgctcgtcc tggtcgagca
    11341 ggtgctggcc aagctcctcg ccgaaaagct ggtgcccgag gcggccctcg ccgcggtgga
    11401 gatgacgggc ctgctcgttt cgctgggacg gaacggcgag gtccccgacc gcctcgccga
    11461 tctccgtgag ctatgcgcca gggactggca gctccgcagg gcctccagca cactgaaccg
    11521 cctggccgcc gcggtcgaag ccggggatcc ggcactcccc cagcgggccc agcgctatgc
    11581 ccaggccctg cgccagacgc tacggctgag ctaccaccgc gtcgagccgc cccccgcggc
    11641 ctgacacccc cgctccccct ccctctctcc cctccccgct cccctccggg agctatcacc
    11701 cccttcagtt cacgtcgcat cgccagcaga tttcaatatc ttccttcgat ttcaaaaaat
    11761 tctcttcttt ttcgtgtgac gttttaccct tctcgtccgt atacatagat agaggggaag
    11821 acagatcctg ccgagccagc cttcccgaga gctcccctcg acagacaacc gctatttcgg
    11881 gcgatcacgc ccaaggagga ggagccgtga acaatcacga cgcgccagag gcgcgcatcc
    11941 tggagaggtt gggagacctg gcccggcagc agagggccct ggtggagcac gtcctgacct
    12001 gcccgagctg taaggccggg tttctcga
//
LOCUS       DASPJI010000271         7965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_309995, whole genome shotgun sequence.
ACCESSION   DASPJI010000271 DASPJI010000000
VERSION     DASPJI010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7965)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309995"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            802..2067
                     /locus_tag="VEL74_07200"
     CDS             802..2067
                     /locus_tag="VEL74_07200"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYG62350.1"
                     /translation="MRKLVRSLLPVCTLVPLLALAGCGGDSNAAASGQGRGGPGGAQG
                     GGRGERRPPGEGLPSRDVRLVRAESASLPRSVAVTGTLAADEEAELGFKVAGRIGSIA
                     VDLGQPVRRGQAIARLVPTDFELRVAQAQNALEQARARLGIPPNGPDRIVPPQETAVV
                     RQAAATLRQAQLTRERMAKLFSEELIPQQDLDAADAALGVADGRYQEAVEEARTRQAL
                     LEQRRSELEIARRQLADSILTAPFDGRVRERRSAIGDYVAAGDTVAVLVRVHPLRLRL
                     AVPEREAAGIRTGQPVRLTVEGDPGTHTGRVARISPSITEDNRTLMIEAEVPNQNGSL
                     RPGAFTRAEIVVEAGAPAVLVPASAVVTFAGIDKVIGVEDGKAAEKRIRIGRRAGDRV
                     EVVEGVEPGDQVIVDPGNLATGQPVQVVG"
     gene            2121..5333
                     /locus_tag="VEL74_07205"
     CDS             2121..5333
                     /locus_tag="VEL74_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYG62351.1"
                     /translation="MQKLAEISIRRPVFAAMIILALVVVGAASYGRLGVDRFPSVDLP
                     TVSVRTLLPGASTEETESLISERIEEAVNTVEGIQELRSITSGGSSFVIATFDLDRDI
                     DVAAQDVRDRVAQLLRQLPDDVLPPVVAKFNNDSSPVLTVALSGQRTLRELTELADKV
                     VKVQLERSAGVGEVQITGGLQRAINVWIDPDRLAAYQLPITQVRNAIARQNTDVPGGN
                     VTGPAQEITLRTMGRFENAEDFNDLVVATVNGQPVRIRDLGYAEDDTKEQRSIARLNG
                     VPTVTLEVRRQSGANTVEVIEGVKEKLALVESQLPTDVQLQVIRDQSRYIYAALHEIN
                     VHLILGSVLASLVVFAFMRSWRSTVIAAVAIPTSVIATFGMMKALDFTLNSVTMLALV
                     LMVGVVIDDAIVVLENIFRFVEEKKMGSFEAAKAATAEIGLAVMATTFSLVVIFVPVS
                     FMSSISGRFLYQFGLTAAVAVLVSLLVSFTLTPMMSARLLRAEDAASGHGGGHEKGDA
                     ARSRTGFYARIDRFYSRMLRFTLAHRLAVALVCLAVMVASIPLFKLVKVEYIPSDVDE
                     SEFDVNVNAPEGTSVPAMNEAMLAVEKELMAIPGVETVLANAGGGFLGGVNQGGAYVR
                     IAPHEERKLSIPRFLKSLARLDPGAAFRGNYSQQDVMMEARQRLRKFSDLRTSVRNIP
                     SFNIGGGNFEIDFVLRGPDLVTLSEYANRLRERSEELGGIVDADTTLKLDKPELRVQV
                     DRARAADLGVDMEDVSTALRLMVGGDEEVSRFRDPAANEDYDVQIRLVESARNTPGAI
                     PQLYVPSSRGELVRLDNLVRLEEKTTAARVDRLDRQRQVSLRAGVGPGYALADRIEAL
                     RQATEEMNLPPGYSTSVGGRAREMERTYQEFAWALTLSIIFMYMILAAQFESLVHPLT
                     ILLSLPLAVPFAMLSLWATGNTLNLYSALGVLVLFGVVKKNAILQIDHMNGLRAKGME
                     RGAAILQANRDRLRPILMTTLALVAGMLPLALGTGPGSEERRAIAVVVIGGQTLSLLL
                     TLLATPVFYSLFDDLRGAVGVKGVLKRLTNGRDRSLREAA"
     gene            complement(5293..5655)
                     /locus_tag="VEL74_07210"
     CDS             complement(5293..5655)
                     /locus_tag="VEL74_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62352.1"
                     /translation="MDHPIPEDTLERFLNGKASRDENRAVVAHLLQGCAACSRALQTL
                     AGRQEAGDSYEEVLDRLEKALAASLDVPVTVEARPARHSGRRSGPPRRAARPAGVGGG
                     GVGVGATPPPAGCGPARW"
     gene            5776..6150
                     /locus_tag="VEL74_07215"
     CDS             5776..6150
                     /locus_tag="VEL74_07215"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HYG62353.1"
                     /translation="MAISKSAPSSAHPSHPSHRRRFPRVSFEKAALVRTIGTDRPVEE
                     FAKTRVLGPGGCMFVSRESLGFSTLMELMISCRGRVIKTDSRVVYEIRRGPGEYEVGV
                     EFLRISPTDRAHVESIVTTRAA"
     gene            complement(6168..>7965)
                     /locus_tag="VEL74_07220"
     CDS             complement(6168..>7965)
                     /locus_tag="VEL74_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62354.1"
                     /translation="VELGAAPESRDKGGFALAGDGRPVWGGEVRAEGAWRLRLHLADV
                     RLPAGARLWVYGEGGEVAPSFGPELIGPGGGIWTPSVGGGAIRLEVVLPAGAEAPEKG
                     PGFTIDRVLELVDVPAAMSKALDCRVDGNCVTPGEFGFVQQLRRAVGVIEYVSGSSSS
                     FCSSGLLNDADPRTTVPYLLTAAHCISEPEEAASAETYFDFVTSGCDGIPPDRGGLPR
                     AVGATLPATVPESDASLLRLSSLPPGRVLLGWTARPLEPGTLLHRLSHALELPQVYSV
                     TRVDPDLAECPTSPRPRYIHSATVRGGAAGGSSGGPAVLGSGHVVGQIRSGCGAESSC
                     PSDVRTIDGAFAVTYPEVRQFLDPVKPCVQGPETLCLADKRFEVRVDWQNQFDGRFGT
                     GKAVRRSDNTGFFYFSSPSNTELILKVLDFGDVVKVFYGQLTDLRFTMTVTDTRTGRV
                     KTYRNGPRECGGIDQEAFPGAAALAAPAAGAVEKAGRRGGCAAGKNALCLLDKRLRVE
                     VDWRNPADGTSGRGVAGPLGQQSGLFSFSNPANVELVVKALPFQDRIAVFYGALSDLD
                     YTITVTDTRTGAVKAYSNPSGNFCGGIDNQAF"
BASE COUNT         1175 a         2874 c         2732 g         1184 t
ORIGIN      
        1 gtgatggcga aggaatcgga gaccggggaa gagggggcag ccgtcgcggc accggccgct
       61 ccgagcgtgg aaacgagtat ggccagcatc agccgaagag tcgatcgcat gggtaggttc
      121 ctttctcgtc aagaccttcc accggaacga acgccggccg cggcggcaac ccctcaccgg
      181 tctttgggat tcagttgtca aggaacaccg cttcgggggc actctacaac gaagcccggc
      241 ccggacacaa gaccgaaatt gacaaaaatg actttgtagg atctcataga gccgcacttg
      301 gaaccgtggt ggacgttctc ggatctgatc cgacccggct gcaccgtctc tcgacccaag
      361 ggtcgagaga ggttcgagga gggtcgagag aagtttgagc agggtcgaga gaggtttgag
      421 cagggtcgag agaggttcga gcagggtcga aacaggtccg gctcgggtcc tgccgtgtgg
      481 gacaggtggc gagcgttctt tgacaatcga tggccccgtt ccgggcgcgg cgaggtggcg
      541 ccgcgcccgg acgggaagtc ccggccctcc cgccgcgttg tagtgctgag accggttacg
      601 ggctccggga gcccgtgccg gtccggggag gcgcgccggg gtgggcaccc cttttgagcc
      661 tcttttacat ttttttacct cactgtccgt tcctgcttcc cgcgggatcg gctacagtcg
      721 ggatgagccc cggtggcgcc gggacgcatc cgcgctggcc tcatcgggcc gcatcggttt
      781 gaatcgtctg gagatctgat cttgaggaag ctcgttcgct ctctgttgcc ggtctgcact
      841 cttgtccccc tgctcgccct cgccggctgt ggcggcgact ccaacgccgc cgcgtcgggc
      901 caggggcgcg gcggcccggg cggtgcccag ggcggcggcc gcggggagag aaggcctccg
      961 ggcgaagggc tcccgtcccg cgacgtgcgc ctggtcaggg ccgagtccgc cagcctcccc
     1021 aggtccgtcg ccgtcaccgg caccctcgcc gccgacgagg aggccgagct cggcttcaag
     1081 gtcgccggac gcatcggcag catcgcggtc gacctcggcc agcccgtgcg ccgcggccag
     1141 gcgatcgccc gcctcgtccc caccgatttc gagctgcgcg tcgcgcaggc ccagaacgcc
     1201 ctggagcagg cccgggcccg cctgggcatc ccgcccaacg gccccgaccg catcgtgccg
     1261 ccgcaggaga ccgccgtcgt ccgccaggca gcggccaccc tgcggcaggc ccagctcacc
     1321 cgcgagcgga tggccaagct gttctccgaa gagctgatcc cccagcagga cctcgacgcg
     1381 gccgacgccg ccctcggcgt ggccgacggc cgctaccagg aggccgtcga ggaggcccgc
     1441 acccgccagg ccctcctcga gcagcgccgc tccgagctcg agatcgcccg gcgccagctc
     1501 gccgattcca tcctcacggc gccgttcgac ggccgcgtcc gcgagcgccg ctccgccatc
     1561 ggcgactacg tcgccgccgg cgacaccgtc gccgtcctcg tgcgcgtcca ccccctgcgc
     1621 ctgcgcctcg ccgtccccga gcgcgaggcc gccggcatcc gcaccggcca gccggtccgc
     1681 ctgaccgtcg aaggcgaccc gggcacccac accggacgcg tcgcccgcat cagcccctcg
     1741 atcaccgagg acaaccgcac cctgatgatc gaggccgagg tgcccaacca gaacggcagc
     1801 ctccggcccg gcgccttcac ccgcgccgag atcgtcgtcg aggccggcgc cccggccgtg
     1861 ctcgtccccg cctccgccgt ggtcaccttc gccggtatcg acaaggtcat cggcgtcgag
     1921 gacggcaagg ccgccgagaa gcgcatccgg atcggccgcc gcgccggcga ccgggtcgag
     1981 gtcgtcgaag gggtcgagcc cggcgaccag gtgatcgtcg atccgggcaa cctggcgacg
     2041 ggccagccgg tgcaggtggt ggggtgaggc agccagcctc gtccttttag atatagagag
     2101 aggcagtcag aggttccttc atgcagaagc ttgccgagat cagtatccga cggccggttt
     2161 tcgcggccat gatcatcctg gccctggtcg tcgtcggcgc cgcgagctac ggccgcctgg
     2221 gcgtcgaccg gttcccctcc gtcgacctgc ccacggtgtc ggtgcgcacc cttctccccg
     2281 gcgcctccac cgaggagacc gagtcgctca tctccgagcg catcgaggag gcggtcaaca
     2341 ccgtcgaggg gatccaggag ctgcgctcga tcacctccgg cggctcctcg ttcgtcatcg
     2401 ccaccttcga cctcgaccgc gacatcgacg tcgccgccca ggacgtgcgc gaccgcgtcg
     2461 cccagctgct gcggcagctc ccggacgacg tgctgccccc cgtggtggcg aagttcaaca
     2521 acgactcctc gcccgtcctc accgtcgcgc tctccggcca gcgcacgctg cgcgagctca
     2581 ccgagctggc cgacaaggtg gtcaaggtcc agctcgagcg ctcagccggc gtcggcgagg
     2641 tgcagatcac cggcgggctg cagcgggcga tcaacgtctg gatcgacccg gaccgcctcg
     2701 ccgcgtacca gctccccatc acccaggtgc gcaacgccat cgcccgccag aacaccgacg
     2761 tgccgggggg caacgtgacc gggccggcgc aggagatcac cctgcgcacc atggggcggt
     2821 tcgagaacgc cgaggacttc aacgacctgg tggtggcgac ggtcaacggc cagcccgtcc
     2881 gcatccgcga cctgggctac gccgaggacg acaccaagga gcagcgctcc atcgcccgct
     2941 tgaacggcgt gcccaccgtc actctggagg tgcggcgcca gtccggcgcc aacaccgtcg
     3001 aggtcatcga gggggtcaag gagaagctcg cgctcgtcga atcccagctc cccacggacg
     3061 tccagctcca ggtgatccgc gaccagtcgc gctacatcta cgcggccctg cacgagatca
     3121 acgtccacct gatcctggga agcgtcctgg cctcgctggt ggtcttcgcc ttcatgcgga
     3181 gctggcgctc gacggtgatc gccgccgtcg ccatcccgac gtcggtcatc gccacgttcg
     3241 gcatgatgaa ggcgctggac ttcaccctca acagcgtcac catgctcgcg ctggtgctca
     3301 tggtgggcgt ggtcatcgac gacgccatcg tggtgctgga gaacatcttc cgcttcgtcg
     3361 aggagaagaa gatgggctcc ttcgaagcgg ccaaggcggc gaccgccgag atcgggctgg
     3421 cggtcatggc caccaccttc tccctggtgg tcatcttcgt gccggtgtcc ttcatgtcca
     3481 gcatctcggg ccggttcctc taccagttcg gcctcaccgc ggcggtggcg gtgctggtgt
     3541 cgctgctcgt ctccttcacc ctgaccccga tgatgagcgc gcgcctgctc cgcgccgagg
     3601 acgcggcttc cggacatggc ggcgggcatg agaaggggga tgcggcccgc tcccgcaccg
     3661 gcttctatgc ccgcatcgac cggttctact cgcggatgct ccgcttcacc ctggcgcacc
     3721 ggctggccgt ggccctcgtc tgcctggcgg tcatggtcgc ctcgatcccc ctcttcaagc
     3781 tggtcaaggt ggagtacatc cccagcgacg tggacgagtc ggagttcgac gtcaacgtca
     3841 acgccccgga ggggaccagc gtgccggcga tgaacgaggc catgctggcg gtcgagaagg
     3901 agctgatggc catccccggc gttgagacgg tgctcgccaa cgccggcggc ggcttcctcg
     3961 gcggcgtcaa ccagggtggc gcctatgtgc gcatcgcccc gcacgaggaa cggaagctct
     4021 ccatcccccg cttcctcaag agcctcgccc ggctcgaccc aggcgcggcc ttccgtggca
     4081 actacagcca gcaggacgtg atgatggagg cccgccagcg cctgcggaag ttctccgacc
     4141 tgcggacgtc ggtccgcaac atcccgtcgt tcaatatcgg cggcggcaac ttcgagatcg
     4201 acttcgtgct gcgcggcccc gacctggtga ccctctccga gtacgcgaac cggctgcggg
     4261 agcgctccga ggagctgggc gggatcgtcg acgcggacac cacgctcaag ctcgacaagc
     4321 ccgagctgcg ggtgcaggtg gaccgcgccc gcgccgccga cctcggcgtc gacatggagg
     4381 acgtctccac ggcgctccgc ctgatggtgg gcggcgatga ggaggtctcc cgcttccgcg
     4441 atccggccgc caacgaggac tacgatgtcc agatccgcct cgtcgagtcg gcgcgcaaca
     4501 ccccgggggc catcccgcag ctctacgtcc ccagcagccg cggggagctg gtgcggctgg
     4561 acaacctggt gcggctggag gagaagacca cggcggcgcg ggtggaccgc ctggaccgcc
     4621 agcggcaggt cagcctgcgc gccggcgtcg gccccggcta cgcgctggcg gaccgcatcg
     4681 aggccctgcg ccaggccacg gaggagatga acctgccccc cggctactcg acatcggtcg
     4741 gcggacgcgc ccgggagatg gagcgcacct accaggagtt cgcctgggcg ctgacgctct
     4801 ccatcatctt catgtacatg atcctggcgg cgcagttcga gagcctggtc cacccgctga
     4861 ccatcctcct ctcgctgccg ctggccgtgc ccttcgcgat gctctcgctc tgggcgacgg
     4921 gcaacacgct gaacctctac tcggcgctgg gggtgctggt gctcttcggt gtggtcaaga
     4981 agaacgccat cctgcagatc gaccacatga acgggctgcg cgccaagggg atggagcgcg
     5041 gcgccgcgat cctccaggcg aaccgagacc ggctgcggcc gatcctcatg accacgctgg
     5101 cgctggtggc gggcatgctg ccgctggccc tcggcaccgg cccgggctcc gaggagcggc
     5161 gggccatcgc cgtggtggtc atcggcggcc agacgctgtc tctgctgctg acgctgctgg
     5221 cgacgccggt cttctactcc ctcttcgacg acctgcgggg cgccgtgggg gtgaaggggg
     5281 tgctgaagcg gctcaccaac gggcgggacc gcagcctgcg ggaggcggcg tagcccctac
     5341 gcccactccc ccacccccca caccggcggg ccgcgccgcg cggcgcggcg gaccggagcg
     5401 ccggccgctg tggcgggcgg ggcgcgcctc gaccgtgacc ggcacgtcca gggaggccgc
     5461 cagggccttc tccaggcggt ccaggacctc ttcataggaa tcgccggctt cctgtcggcc
     5521 ggccaatgtc tgcaaggcgc gggagcaggc ggcgcagccc tggaggagat gggcgacgac
     5581 ggcacggttc tcgtcccgcg acgccttgcc gttcaggaac ctctcgaggg tgtcttccgg
     5641 gatggggtgg tccatgggcg tcctccttct tcaagaggag gtgacggacc ggggacatcc
     5701 atttcatccc ccattgatct ccggcgatat ttcgcttaga gtcattcgtc gatagacatc
     5761 tccaggaggc tccccatggc gatctcgaag tcggctccga gctccgctca cccctcgcac
     5821 ccctctcacc ggcgccgttt cccccgcgtc tcgttcgaga aggccgcgct ggtgcggacc
     5881 atcggcacgg accgccccgt cgaggagttc gccaaaacgc gggtgctcgg ccccggcggc
     5941 tgcatgttcg tcagccgcga gtcgctggga ttctccaccc tgatggagct gatgatctcc
     6001 tgccgcgggc gggtcatcaa gaccgacagc cgggtggtct acgagatccg ccggggcccc
     6061 ggggagtacg aggtgggcgt cgagttcctc cgcatcagcc ccaccgaccg ggcgcacgtg
     6121 gagtcgatcg tcaccacccg ggcggcctag aacgccagcc cgatcctcta gaacgcctgg
     6181 ttgtcgatcc cgccgcagaa gtttcccgac gggttcgagt aggccttgac cgcgccggtc
     6241 cgggtgtcgg tcacggtgat ggtgtagtcg aggtccgaga gggcgccgta gaagacggcg
     6301 atccggtcct ggaagggcag cgccttcacc accagctcga cgttggccgg gttgctgaac
     6361 gaaaagaggc ccgactgctg gccgagcggg ccggcgacgc cgcggccgga ggtgccgtcg
     6421 gcggggttgc gccagtccac ctcgacccgc aggcgcttgt ccagcagaca gagggcgttc
     6481 ttcccggcgg cacagccgcc gcggcgcccg gccttctcca cggcacccgc cgccggcgcg
     6541 gcgagcgccg ccgccccggg gaacgcctcc tggtcgatgc cgccgcactc cctgggaccg
     6601 ttgcggtagg tcttgaccct gcccgtccgg gtgtcggtga ccgtcatggt gaagcgcagg
     6661 tcggtgagct ggccgtagaa gaccttcacc acgtcgccga agtccaggac cttgaggatc
     6721 agctcggtgt tggacggcga cgagaagtag aagaagccgg tgttgtcgct gcgccggacg
     6781 gccttgccgg tgccgaaccg gccgtcgaac tggttctgcc agtccacccg gacctcgaaa
     6841 cgcttgtcgg ccagacagag ggtctcgggg ccctggacgc acggcttcac cgggtcgagg
     6901 aactgccgta cctcgggata ggtgacggcg aaggcgccgt cgatggtccg cacgtccgac
     6961 gggcaactgc tctcggcgcc gcagccgctg cggatctgcc ccacgacgtg gccgctgccg
     7021 aggaccgccg ggccgccgga gctgccgccg gccgcgccgc cgcgcacggt ggcggagtgg
     7081 atgtaccggg gccgcggcga cgtggggcac tccgcgaggt cgggatcgac ccgggtgacg
     7141 ctgtagacct ggggcagctc caatgcgtgc gacaggcggt ggagcagggt cccgggctcc
     7201 agcgggcgcg ccgtccagcc gagcagcacg cggccggggg gcaggctgga gaggcgcagc
     7261 aggctcgcgt cgctctccgg caccgtggcg ggcagggtcg cccccaccgc ccgcggcagc
     7321 ccgccccggt ccggcgggat gccgtcgcac cccgaggtga cgaagtcgaa gtaggtctcc
     7381 gccgacgccg cctcctcggg ctcgctgatg cagtgggcgg cggtcagcag gtaggggacg
     7441 gtggtgcggg ggtccgcgtc gttcagcagg ccgctgctac agaagctgct cgagctgccg
     7501 ctgacgtact cgatgacacc gaccgcgcgc cggagctgct ggacgaaccc gaactcgccc
     7561 ggagtgacac agttgccgtc cacccggcag tccagagctt tggacatggc cgcggggacg
     7621 tccaccagct ccagcacccg gtcgatggtg aagcccggcc ccttctccgg agcctcggcg
     7681 cccgccggga gcacgacctc cagacggatg gcgccaccgc cgacgctggg ggtccagatc
     7741 ccgccgccgg gcccgatcag ctcggggccg aaggaggggg ccacctcgcc gccttcgcca
     7801 tagacccaga ggcgggcgcc cgccggcagc cgcacgtccg ccaggtggag ccgcagccgc
     7861 caggcccctt cggcgcgcac ctcgccgccc cacaccggcc ttccatctcc ggccagagcg
     7921 aagcccccct tgtcccggga ttccggcgcg gcgccgagct ccacc
//
LOCUS       DASPJI010000272         7005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_529947, whole genome shotgun sequence.
ACCESSION   DASPJI010000272 DASPJI010000000
VERSION     DASPJI010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529947"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4828)
                     /locus_tag="VEL74_07225"
     CDS             complement(<1..4828)
                     /locus_tag="VEL74_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62355.1"
                     /translation="MSKQNIEDIYPLSPAQQGMLMVLLLSGYRSEVYFEQVVATLEGR
                     LDLAAWQRAWQEVVRRHPALRTQLVWERREQPLQVVRREVELPWQELDWSGLPEAERE
                     ERLAAFLRDDHARGFDLGKPPLMRIAMIRWQVNGQEDAYRLVWSFHHLVFDGWSISLV
                     LSEAINLYSAYRAGRAPNLPAPRPYRSYIGWLQRRDPVRSEAFWRQELAGFTDPTPLP
                     LAGLLDGDGVSPDGDGWASTQEKRALTPEITAALDRLARRHGLTLNTVVQGAWGLLLS
                     RLTGGSDVVFGAVVSGRPAEVEGIETMAGLFINALPVRVRVDPARELAPWLAELQERQ
                     SEQREYEHCLLEQVQAWSEVPRQTPLFESLLVFQNYPVDPLGLGDDAGFTMRDSRLKE
                     STHYPLTMYVTPEDGRLQLRLAYHVQRFGAGAARRLLGWFAALLEGIARTASERIAEE
                     EGPRIGELPLLSAEERGELLAAAAGPVGPAAWPVLRLFQEQAARTPQAPAVISAQGEL
                     TYAELATRARRLGLHLRRLGVGAESVVGLCVERSLDMVVGMLGVLEAGGAYLPLDPAY
                     PVERLGFMLRDSGARVLLTQDALVDRLPEAVPGLLGVVCLDTDWPMIDRAEESPAPAG
                     GCGVHNPVYIIYTSGSTGQPKGVVVEHASLANYVASAADDYGIGPDDRVLQFASMSFD
                     TSAEEIYPCLTRGATLVLRDGAMASSPDGFAREVERLGITVLDLPTAYWHELVAEMER
                     QGFGMPDSLRLVILGGEQAQAARFAAWRARVGDRVRLVNTYGPTEATIVTTHRDLTGE
                     VGFPGEIPIGRPVRNARAYVVARGGWDLAPAGVDGELWIGGAGLARGYLGRPDLTAER
                     FVPNPFLGSDTEGGRPGERLYRTGDLVRLLPDGDLEFRGRVDHQVKIRGFRIELGEVE
                     AALRRLPGVREAVAGIHQEDAGDKRLVAWVETGEGERPTVAALRQGLKGLLPDHMVPT
                     AFVILEALPLTPSGKLDRRALPAPDAAGQDAGGDFAAPRSPVEEVLAGIWSEVLKVDR
                     VGIHDDFFVLGGHSLLVGQVVTRVRQALAVELPLADVFKQPTVARLAELIEQGQLGAD
                     LPELPPIVRQPRDGRTFPLSFPQERVWFLDQLSVGGNIAYNFQVAIWFQGPLDVPVLE
                     RSLGELIRRHEVLRTSFPAADGLPVQSIHPAAPVSLELVDLSDYPEAEREEMAETWID
                     ATVRDPFDVSTGPLIRWRLLRLAPDHHALVQVEHHFVHDGWSFGVLLRELKAIYAAFS
                     QGEPSPLPELPVQYADFSVWQRQWLEGEVMERLLGFWRQKLAGHPPALEIPGDRPRPA
                     RPKFQGRVEMFLVRPDLYEALRRFGRKEGFTFYMTMLSGFFTLLHRYTGQQDVVIGSS
                     NANRRAKEIEGMIGMVVNTLVLRGDLAPEAGAEPDFRKVLARVRELTLEVYAHQDMPF
                     ERLVKDLKIERQIGRNPLFQIMFNFHNASVPDLDFGGGLSAATNVRANRSAKMDMNII
                     VVPRSEQRLGLAESEIDRHAVLHWEWNTDLYDFQTIERMVGHYQTLLEGVVASPETPL
                     GELPILTAAEREQLTAWAETGTAYPREATIPGLFEACAEASPGSVAVELGDESLTYA"
     gene            complement(4872..>7005)
                     /locus_tag="VEL74_07230"
     CDS             complement(4872..>7005)
                     /locus_tag="VEL74_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG62356.1"
                     /translation="GLTAERFLANPLGQQAGNRVYRTGDLGRYLPDGNAEFAGRADFQ
                     VKIRGFRIELGEIEAALDRFPGVKQCVVVAREDRPGDRRLAAYLVVDGEAPPARELRA
                     FLEECLPDYMVPSAFVALAALPLTQTGKIDRKALPAPDSAWTDLGRDYVAPRNQIEEV
                     LAGVWAEALRRERVGIYDNFFELGGDSILILQVVSKSQKRGLRFAPRQLFQHQTVAEL
                     AEVVDAADLLAARAEDSGPVPLTPPQIRFLESTGTCCPSGWSEAILVELPVGTAAEQV
                     EQALAVLGTAHDAFRLRIERLTPEGVCRQSLTAPNCTPLIPLERCELPADPAEREAVL
                     AERVAEARSRLDLAAGPVARATFFRNSTGPDRLLLAAHPLVVDASSWPILLAGLADAC
                     GRLNRGDSMEAAPRTASFRRWATALAERAQSGAGRAGVKEWSAALRPEPPRLPVDFRA
                     AGDAGDGGNVGDSERRALSVWLDEAATRDLLAEVPKTYGTTTQEVLLAALAEAFAPWT
                     GSRRLAVEVEGGGREGLLGGGLDVSRTVGCLDPVFPLSLEVRDNPGESLKETKERLRR
                     AARQAVDYRSGAAGTAGAAGAGVAEVGLRWRERIEVRPEGGPLWSAEPLPAGRPDEAP
                     RRHLLEVEALPAGSRLRVDWSWDARVHRPATVQALADRFLQALRSSIQHCQTPESAGF
                     TPSDFPEAGMNQADLDDLLAELSESFDS"
BASE COUNT         1012 a         2569 c         2524 g          900 t
ORIGIN      
        1 cggcataggt gaggctctcg tcgccgagct ccacggccac cgacccgggc gacgcctcgg
       61 cgcaggcctc gaacagcccg gggatcgtcg cctcccgcgg ataggcggtg ccggtctccg
      121 cccaggccgt gagctgctcg cgctcggccg ccgtgaggat cggcagctca cccagcggcg
      181 tctcggggct cgccaccacc ccctcgagca gcgtctggta gtgccccacc atgcgctcga
      241 tggtctggaa gtcgtagagg tcggtgttcc actcccagtg cagcacggcg tgccggtcga
      301 tctcgctctc ggcgagcccg aggcgctgct cggaccgcgg cacgacgatg atgttcatgt
      361 ccatcttggc cgagcggttg gcccggacgt tggtcgccgc gctcagacca ccgccgaagt
      421 ccaggtcggg gacgctggcg ttgtgaaaat tgaacatgat ctggaagaga gggttgcggc
      481 cgatctggcg ctcgatcttc aggtccttga ccagccgctc gaagggcatg tcctggtggg
      541 catagacctc cagcgtcagc tcgcgcaccc gggcgaggac cttgcggaaa tcgggctcgg
      601 cgccagcttc cggagctaag tcgccgcgca gcactagggt gttgaccacc atgccgatca
      661 tcccctcgat ctccttggcg cggcggttgg cgttgctgga gccgatgacc acgtcctgct
      721 ggccggtgta gcggtggagc agggtgaaga agccggagag catggtcatg tagaaggtga
      781 agccctcctt gcggccgaac cggcgcaacg cctcgtagag gtcgggccgc accaggaaca
      841 tctcgacccg cccctggaac ttggggcgcg ccggccgcgg ccggtcgccg gggatctcca
      901 gcgccggcgg gtggccggcc agcttctggc gccagaagcc gaggagacgc tccatgacct
      961 ccccctccag ccactggcgc tgccagacgg agaagtcggc gtactgcacc ggcagctccg
     1021 gcagggggga cggctcgccc tgggagaaag cggcatagat cgccttgagc tcgcgcagca
     1081 ggacgccgaa ggaccagccg tcgtgcacga agtggtgctc cacctgcacc agggcgtggt
     1141 ggtcgggcgc caggcgcagg aggcgccagc ggatcagcgg cccggtggag acgtcgaagg
     1201 gatcgcgcac ggtggcgtcg atccacgtct cggccatctc ctcgcgctcc gcctcgggat
     1261 agtccgacag gtccaccagc tcgagagaca ccggcgcggc gggatggatc gactgcaccg
     1321 gcagcccgtc ggccgccggg aagctggtgc gcagcacctc gtggcggcgg atgagctcgc
     1381 cgaggctccg ctccagcacc ggcacgtcga gcggcccctg gaaccagatg gccacctgga
     1441 agttgtaggc gatgttgccg cccaccgaga gctggtcgag gaaccagacc cgctcctgcg
     1501 ggaaggaaag ggggaaggtc cggccgtcgc gcggctggcg gacgatcggc ggcagctccg
     1561 gcaggtccgc cccgagctgg ccctgctcga tcagctcggc gagacgcgcc accgtcggct
     1621 gcttgaagac gtcggccagg ggcagctcca ccgccagcgc ctggcggacg cgcgtcacca
     1681 cctgccccac cagcagcgag tggccgccga ggacgaagaa gtcgtcgtgg atccccaccc
     1741 ggtcaacctt caggacctcg ctccagatgc cggccagcac ctcctcgacc gggctgcgcg
     1801 gcgcggcgaa gtcgccgcca gcatcctggc cggccgcgtc cggcgccggc agggcgcggc
     1861 ggtcgagctt gccgctgggg gtcaggggga gggcctcgag gatcacgaag gccgtgggga
     1921 ccatgtggtc ggggagcagc cccttgagac cctggcgcag ggccgccacc gtggggcgct
     1981 ccccctcacc ggtctcgacc caggcgacga gccgcttgtc gcctgcgtcc tcctgatgga
     2041 tgccggcgac cgcctcccgc accccgggca ggcggcgcag ggcggcctcg acctctccca
     2101 gctcgatgcg gaagccccgg atcttcacct gatggtcgac gcggccgcgg aactccaggt
     2161 cgccgtccgg cagaaggcgg accagatcgc cggtgcgata gagccgctcg ccgggccgcc
     2221 ccccctcggt gtcggagcca aggaacgggt tggggacgaa gcgctcggcg gtcaggtcgg
     2281 ggcggcccag gtagccgcgg gccaggccgg cgccgccgat ccacagctcg ccgtcgacgc
     2341 ccgcgggggc cagatcccag ccgccccggg caaccacgta ggcgcgggcg ttgcgcaccg
     2401 gccgtccgat gggaatctcg cccgggaagc cgacctcccc cgtcaggtcg cggtgggtgg
     2461 tgacgatggt ggcctcggtg gggccgtagg tgttgaccag ccgcacccgg tcgcccaccc
     2521 gcgctcgcca ggcggcgaag cgcgccgcct gggcctgctc gccgccgagg atcaccagac
     2581 ggaggctgtc cggcatgccg aagccctgcc gctccatctc ggccaccagc tcgtgccagt
     2641 aggcggtggg caggtcgagc acggtgatcc ccaggcgctc gacctcgcgg gcgaagccgt
     2701 cgggcgagga ggccatggcg ccgtcccgca gcaccagcgt ggcgccgcgg gtcaggcagg
     2761 gatagatctc ctcggcgctg gtgtcgaagc tcatggacgc gaactgcagc acccggtcgt
     2821 ccgggccgat gccgtagtcg tccgcggccg acgccacgta gttggccagc gacgcgtgct
     2881 ccaccaccac gcccttgggc tggccggtcg agcccgacgt atagatgatg tagaccgggt
     2941 tgtggacgcc gcagccgccg gccggcgccg ggctctcctc ggcgcggtcg atcatcggcc
     3001 agtcggtgtc caggcagacc acgcccagaa gccccggcac cgcctccgga aggcggtcca
     3061 ccagcgcgtc ctgggtgagc aggacgcgcg ccccggagtc gcgcagcatg aagccgaggc
     3121 gctccaccgg gtaggccggg tcgagcggca ggtaggcgcc gccggcctcg agcacgccca
     3181 gcatgcccac caccatgtcc agcgagcgct ccacgcagag gccgaccacc gactcggccc
     3241 cgacgcccag gcgccgcagg tggaggccca gacgccgcgc ccgggtggcg agctcggcgt
     3301 aggtgagctc cccctgggcg gagatcacgg ccggcgcctg cggcgtgcgc gccgcctgct
     3361 cctggaagag gcggagcacc ggccaggccg cggggcccac cggaccggcg gcggcggcca
     3421 gcagctcgcc ccgctcctcc gccgacagca gcggcagctc gccgatccgc ggcccctcct
     3481 cctccgcgat cctctcggaa gcggtgcggg cgatcccctc gagcagggcc gcgaaccagc
     3541 ccaggagccg ccgcgccgcg ccggcgccga agcgctgcac gtggtaggcg agccggagct
     3601 ggaggcgccc gtcctcgggg gtgacgtaca tcgtcagcgg atagtgggtg gactccttga
     3661 gccgcgagtc ccgcatggtg aagccggcgt cgtcgcccag ccccagcggg tccacgggat
     3721 agttctggaa gaccaggagg ctctcgaaga gcggcgtctg gcgcggcacc tcgctccagg
     3781 cctgcacttg ctccagcagg cagtgctcgt actcgcgctg ctcgctctgg cgctcctgca
     3841 gctccgccag ccagggcgcc agctcgcggg cggggtcgac ccgcacgcgc accggcagcg
     3901 cgttgatgaa caggccggcc atggtctcga tcccctcgac ctcggccggg cggcccgaga
     3961 ccacggcccc gaataccacg tcggagccgc cggtgaggcg ggagaggagg agaccccagg
     4021 ccccctggac caccgtgttc agggtcaggc cgtgccgccg cgccagcctg tcgagggcgg
     4081 cggtgatctc cggcgtcagc gcccgcttct cctgggtgga ggcccagccg tcgccgtccg
     4141 gagagacgcc gtccccatcc aggaggccgg cgagcggcag gggggtggga tcggtgaagc
     4201 cggccagctc ctgccgccag aacgcctcgg accggaccgg gtcgcgccgc tgcagccagc
     4261 cgatgtagga gcggtagggg cgcggcgccg gcaggttcgg agcccgtccg gcgcgatagg
     4321 cggagtagag gttgatcgcc tcggagagga ccagcgagat ggaccagccg tcgaagacca
     4381 ggtgatggaa gctccacacc agccggtagg cgtcctcctg gccgttcacc tgccagcgga
     4441 tcatggcgat gcgcatcagc ggcggcttgc cgaggtcgaa gccgcgggca tggtcgtcgc
     4501 gcaggaaggc ggccagccgc tcctcccgct cggcctccgg cagaccgctc cagtcgagct
     4561 cctgccaggg cagctcgacc tcgcggcgca ccacctggag cggctgctcc cggcgctccc
     4621 agacgagctg cgtgcgcaac gccggatggc gccgcaccac ctcctgccag gcgcgctgcc
     4681 aggcggcgag gtcgagacgg ccttccaggg tggcgaccac ctgctcgaag tagacctccg
     4741 agcggtaccc cgacagcagg aggaccatga gcatgccctg ctgggctgga gatagcgggt
     4801 agatgtcctc gatgttctgc ttgctcatac gacctttctg ccgggccgga atcggggccc
     4861 gtcctccctc ttcaagaatc aaaagactcc gacagctccg ccaggagatc atccagatcc
     4921 gcctggttca tgcccgcctc cgggaaatcg gaaggggtga acccggcgct ctccggcgtc
     4981 tggcagtgct ggatcgacga cctcagagcc tgcaggaagc ggtcggccag ggcctggacc
     5041 gtcgccggcc ggtggacccg cgcgtcccag ctccagtcca cccgcaggcg cgagccggcg
     5101 ggcagggcct ccacctccag gagatgccgc cgcggcgcct cgtccggccg gcccgcgggg
     5161 agcggctcgg cgctccagag gggtcccccc tccggccgca cctcgatccg ctcgcgccag
     5221 cgcagcccga cctcggccac cccggcgcca gcggcaccag cggtgccagc ggcacccgaa
     5281 cggtaatcga cggcctggcg cgcggcccgg cgcagtctct ccttggtttc cttgagtgac
     5341 tccccgggat tgtcgcgcac ttccagggaa agtggaaaga ctggatcgag gcacccgacg
     5401 gtccgggaga cgtcgaggcc ccccccgagg agaccttcgc ggcctccccc ctccacctcc
     5461 accgccagac ggcgcgagcc ggtccagggg gcgaaggcct cggccagcgc cgccaggagc
     5521 acctcctgcg tcgtggtgcc gtaggtcttc gggacctccg ccagcagatc gcgggtggcc
     5581 gcttcgtcca gccagaccga gagggcccgc cgctcgctgt cgccgacatt gccgccgtct
     5641 cccgcatcgc cggcagccct gaagtccacc ggcagccggg gcggctccgg gcggagcgcc
     5701 gcactccact ccttcacccc cgcgcggccg gccccggact gggcccgttc ggccagcgcc
     5761 gtggcccagc gccggaagga ggcggtccgc ggcgcggcct ccatggagtc gccacggttc
     5821 agccgcccgc aggcgtccgc cagcccggcg agcaggatcg gccaggagga ggcgtccacc
     5881 accagcgggt gggcggcgag gagaagccgg tccggtccgg tgctgttcct gaagaaggtg
     5941 gcgcgggcga ccggcccggc cgcgagatcg agccggctcc gcgcctcggc cacccgctcg
     6001 gccaggaccg cctcgcgctc cgccgggtcg gcgggcagct cgcaacgctc cagggggatg
     6061 agcggcgtgc agttcggcgc ggtgagggac tgccggcaga ccccttccgg cgtcagccgc
     6121 tcgatgcgca gacggaatgc gtcgtgcgcc gtgcccagaa cggccagtgc ctgctccacc
     6181 tgctccgcgg ccgtgccgac gggcagctcc accaggatcg cctcgctcca gccggaggga
     6241 cagcaggtcc cggtggattc caggaatcgg atctgcggcg gcgtcagcgg caccggaccc
     6301 gagtcctcgg cacgggcggc gagcaggtcg gcggcgtcca ccacctcggc cagctccgcc
     6361 accgtctggt gctggaagag ctgccggggg gcgaagcgca ggccccgctt ctggctcttc
     6421 gacaccacct ggaggatgag gatcgagtcc ccccccagct cgaagaagtt gtcgtagatg
     6481 cccacccgct cccggcgcag ggcctcggcc cagacgccgg ccagcacctc ctcgatctgg
     6541 ttgcgcggcg ccacgtagtc ccgtcccagg tcggtccagg cgctgtccgg cgccggcagg
     6601 gccttgcggt cgatcttgcc cgtctgggtg agggggaggg cggccagggc gacgaacgcg
     6661 gacggcacca tgtagtcggg gaggcactcc tcgaggaagg cgcgcagctc gcgggccggc
     6721 ggcgcctcgc cgtcgaccac caggtaggcg gccaggcggc ggtcgcccgg ccggtcctcg
     6781 cgcgccacca cgacgcactg cttcacgccg gggaagcggt ccagagccgc ctcgatctcc
     6841 cccagctcga tccggaagcc gcggatcttc acctggaagt cggcgcggcc ggcgaactcg
     6901 gcgttgccgt ccggcaggta gcggcccagg tcgcccgtgc ggtagacgcg gttccctgcc
     6961 tgctgtccca aaggattggc gaggaaccgc tccgccgtca ggccc
//
LOCUS       DASPJI010000273         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_189765, whole genome shotgun sequence.
ACCESSION   DASPJI010000273 DASPJI010000000
VERSION     DASPJI010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189765"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2190
                     /locus_tag="VEL74_07235"
     CDS             <1..2190
                     /locus_tag="VEL74_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYG62357.1"
                     /translation="EIKLHTARGGLATRLSRSVSLLGGKVAGLVRRSGRREAETEGGV
                     EVVRYRNRLGGTVAGILDANFDTSDPAIRPDVAVVVAPAILKRKEVFGLLARTLVDDL
                     DRDGRKVVVLRFDACHIVGESTMDPRLVAEGRPYYNWTFGHFAADIEASLQYLERRFR
                     PARRTLVSLSLSAIPARKVVAGCRDAPVDLWIAPYGCPDAQDVMRNYLAGLDLFDAYR
                     KGEGPEAVLIHGRPIRGASLYGQAMEAGMAFLDEARRDMSRIRVPVSWIVGTYDCWVS
                     RSRVRAMLDAPGGGVREVFECATGHVLKSGAEAIELFKLVAESVSKHLFGADRPAREP
                     NLARFAGQSEAEWARVRRQELTDAEGFWKEHLFGSLPGSPVPASQEVGYDVLLEHPEY
                     PRFLARQAELLDPQPGEHLADFGCGTGNLLLAMLDALPESGPPASVTFLDLVPEGVER
                     TAAKVREHFAARGAAAPLCRGLVADLEVSRLAAVADFLNGHLHGVEGLVDRVEGFDRV
                     TARKLAAGYGKELHAILRGEPAEPRRIGELCPGLGEREVEVVLEIGRAVRFLAGRLLP
                     ADLRPGVTSARTAADLGFRHLRFGEATPALRLGLPDACFHRIGSSLVLPYLYDPLASL
                     REMHRLLVPGGTLVVSSIRPNFDPSKLYSEGAGILAGQGAERKLEALRHFASMVSRLI
                     ELEEDGRFRFLDGAALKALVAEAGFSRVHAFQAFGSPPVAVIVRAEK"
     gene            2187..>3250
                     /locus_tag="VEL74_07240"
     CDS             2187..>3250
                     /locus_tag="VEL74_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HYG62358.1"
                     /translation="MSDGSPGVRSPRLPGFEEAFRRESAELFVERARFVVLSALVLYP
                     SFWLLDLLVAPELAGRFLLYRAEVAALYILILAALRGRAAQRLAPPLVFAGTFASVAG
                     ISLMAAEMGGFASNYFAGNLIVLFVIGVFIPWEPAAAALLSLLIIATYTGINLWAHGS
                     SREMNAPLFFLAGSAVFTCLAVLSSRITRRRDLSLRLRLEKANEELKELDEAKSRFFA
                     NVSHELRTPLTLLIGPLESLVAEEPDEGRVLVYEAMAANARRLLRQVDALLDTAKLEA
                     GRLLLEPVQADLGRLLGELVAAAEPHAARRRIRLEAAGLEDLPRFPFDPPKLEIVAAN
                     LLSNAVKFTPDGGRVTVRAEA"
BASE COUNT          394 a         1207 c         1149 g          500 t
ORIGIN      
        1 gagatcaagc tccacaccgc ccgcggcggc ctcgccaccc gcctctcgcg ctccgtctcg
       61 ctgctgggag gcaaggtggc ggggctggtg cgccgctccg gccgccgcga agccgagacc
      121 gagggcgggg tggaggtggt ccgctaccgc aaccgcctgg gcggcaccgt cgccggcatc
      181 ctcgacgcga acttcgacac ctccgatccc gcgatccggc ccgacgtggc cgtggtcgtc
      241 gcgcccgcca tcctcaagcg caaggaggtc ttcggcctgc tcgcccgcac cctggtggac
      301 gacctggacc gcgacggccg caaggtggtg gtcctgcgct tcgacgcctg ccacatcgtc
      361 ggggagagca ccatggaccc gcggctcgtc gccgaaggcc gtccttatta caactggacc
      421 ttcggccact tcgccgccga catcgaggcc tcgctgcagt acctcgagcg gcgcttccgg
      481 ccggcccggc ggacgctggt gagcctctcc ctgtcggcca tcccggcgcg caaggtcgtc
      541 gccggctgcc gggacgctcc cgtggacctc tggatcgccc cctacggctg ccccgacgcc
      601 caggacgtca tgcgcaacta tctggccggg ctcgacctgt tcgacgccta ccgcaagggg
      661 gaggggccgg aggcggtgct gatccacggg cggccgatcc gcggcgcctc gctctatggc
      721 caagccatgg aagccggcat ggcgttcctc gacgaggcgc ggcgcgacat gagccgcatc
      781 cgcgtcccgg tcagctggat cgtggggacc tacgactgct gggtgagccg ctcgcgcgtc
      841 cgcgccatgc tggacgcgcc gggcggcggc gtgcgcgagg tcttcgagtg cgccaccggg
      901 cacgtgctca agagcggcgc cgaggccatc gagctgttca agctggtggc ggagtcggtc
      961 tccaagcacc tgttcggcgc cgaccgcccg gcgcgcgagc ccaacctggc ccgcttcgcc
     1021 gggcagagcg aggccgagtg ggcgcgcgtc cggcgccagg agctgaccga cgccgagggg
     1081 ttctggaagg agcacctgtt cggttcattg cctggctcac cggtgccggc cagtcaggag
     1141 gtgggctacg acgtgctcct ggagcatccg gagtacccgc gcttcctggc ccgccaggcc
     1201 gagctgctgg acccccagcc cggcgagcac ctggccgact tcggctgcgg caccggcaac
     1261 ctcctgctgg cgatgctcga cgcgctgccg gaatccggac ctcccgcctc ggtcaccttc
     1321 ctcgacctgg tgccggaggg ggtggagcgc accgcggcca aggtgcggga gcacttcgcg
     1381 gctcgtgggg cggcggcgcc cctctgccgc ggcctggtgg ccgacctcga ggtgtcgcgc
     1441 ctcgccgcgg tagcggactt cctgaacggc cacctgcacg gcgtcgaggg gctggtggac
     1501 cgggtcgaag ggttcgaccg ggtcacggcg cgcaagctgg ccgccggcta cggcaaggag
     1561 ctgcacgcca tcctgcgcgg cgagccggcc gagccgcggc ggatcggcga gctctgcccc
     1621 ggcctcggcg agcgcgaggt ggaggtcgtc ctcgagatcg gccgcgccgt ccgcttcctg
     1681 gcggggcggc tcctgcccgc cgacctgcgg ccgggggtga cttcggcgcg tacggcggcg
     1741 gacctcggct tccggcatct gcggttcggc gaggccacgc ccgccctccg gctcggcctc
     1801 cccgacgcct gcttccaccg gatcggctcg tcgctcgtcc tgccgtacct ctacgacccg
     1861 ctcgccagcc tgcgcgagat gcaccgcctg ctcgtgccgg gcggcaccct ggtcgtctcg
     1921 tcgatccggc cgaacttcga cccctccaag ctctacagcg agggggcagg gatcctggca
     1981 ggacagggtg ctgagcgcaa gctggaggcg ctgcgccact tcgccagcat ggtgagccgg
     2041 ctcatcgagc tggaggaaga cggccgcttc cgtttcctcg acggcgcggc gctcaaggcg
     2101 ctggtggccg aggccgggtt cagccgcgtc cacgcgttcc aggccttcgg ctcgccgccg
     2161 gtggcggtga tcgtacgggc agagaagtga gcgacggctc acccggcgtc cgctccccac
     2221 ggctgccggg attcgaggag gcgttccgcc gcgagagcgc cgagctgttc gtggagaggg
     2281 cgcggttcgt cgtcctctcc gccctcgtcc tctacccctc gttctggctg ctcgacctgc
     2341 tggtggcccc ggagctcgcc ggccgattcc tgctgtaccg cgccgaggtg gccgccctct
     2401 atatcctgat cctcgccgcc ctgcgcggcc gtgccgccca gcgcctggcg ccgccgctgg
     2461 tcttcgccgg caccttcgcc tcagtggcgg ggatctccct gatggcggcg gagatggggg
     2521 ggttcgccag caactacttc gccggcaacc tcatcgtcct cttcgtcatc ggcgtcttca
     2581 tcccctggga gccggcggcg gcggcgctgc tgtcgctgct gatcatcgcc acctatacgg
     2641 gtatcaacct gtgggcgcac ggttcgtccc gggagatgaa cgcccccctc ttcttcctcg
     2701 ccggctcggc ggtcttcacc tgcctcgccg tgctgtcgag ccgcatcacg cggcggcggg
     2761 acctgtcgct gcggctgcgg ctggagaagg ccaacgagga gctcaaggag ctggacgagg
     2821 ccaagagccg cttcttcgcc aacgtcagcc acgagctgcg cacccccttg acgctgctga
     2881 ttggccccct cgagagcctg gtggcggagg agccggacga gggccgcgtc ctggtctacg
     2941 aggcgatggc ggccaacgcc cgtcgcctgc tgcgccaggt ggacgcgctg cttgacaccg
     3001 cgaagcttga ggccggccgg ctgctcctcg agccggtgca ggctgacctc ggccgcctcc
     3061 tgggcgagct ggtcgccgcc gccgagccgc acgccgcccg gcggaggatc cgtctggaag
     3121 ccgccggcct ggaggacctg ccccggttcc ccttcgatcc ccccaagctg gagatcgtgg
     3181 cggcgaacct cctctccaac gccgtcaagt tcacgcccga cggtggccgg gtgacggtac
     3241 gggccgaggc
//
LOCUS       DASPJI010000274         5420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_131727, whole genome shotgun sequence.
ACCESSION   DASPJI010000274 DASPJI010000000
VERSION     DASPJI010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131727"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VEL74_07245"
     CDS             <1..339
                     /locus_tag="VEL74_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HYG62359.1"
                     /translation="LRRGAGRIPGTALPWEEGNVGVAAHRDRFFRGLKDIQKDDLVTL
                     TTVHGTYQYRVESTEVVTPRDTHVLADTGEDTLTLVTCYPFYYVGSAPKRFIVRAQRI
                     EGGETAGGVS"
     gene            396..1169
                     /locus_tag="VEL74_07250"
     CDS             396..1169
                     /locus_tag="VEL74_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62360.1"
                     /translation="MPRRRSWRPRWLSVPGLTGFLLALLLVGAVPGSASEPPLARLLS
                     PAMGDELAVGSMATVEWEPAAGLAAMERAEEWEAFLSVDGGATYPLRITPHLDLSIRR
                     FSFRVPDLPSRQARLLLRFGDERREEVDCETRQIFAIHRPPGYVPLHSPKIAFTRGEA
                     ARAGDAGVVAWVEGARDGSGLREVVSVADPGASLESARPAGPLLLPLLWPAPVRAALA
                     PPAVAPAGSYPEGRAIPEPSPAPAGASAPVRLLIHRFNE"
     gene            1312..3522
                     /locus_tag="VEL74_07255"
     CDS             1312..3522
                     /locus_tag="VEL74_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYG62361.1"
                     /translation="MRKLQGAPPGLPFAFLLFVLTLVPSLAAAFEGRLLLPDGTPAAG
                     YQVSVVGRSISVPVGRDGRFRIDPDPSLPFRLVAISPSGELSPPLDVEALPEEVLDLT
                     LPPAFRESVTVASGVAPGIDSPPAAATVMIAQEDLEQRRPDRLIEALEGVAGVSSTDQ
                     TTTGVPVIRGLARGRTLILLDGGRVTAERRAGPSASFLDPFTLGSVEVSRGPGSVAYG
                     SDAFGGVINARSRFPEPGQGRALRFQLDKGFGGSDEESVGLEGSTDLWGGALLGQVTW
                     RQGDDGEAGGGETIDLSSFEDRSGALRYSRQTRLGYLRAGFSAADAEDVGKPASDSDI
                     TRTIYPKETSRRFHLSLDTGPLGGWDSLEMGLFFGSYRLVLDRDRLATATSTRLIERS
                     DVDSNDGSVRAVASRGVLGGRLQLGFESASRFGLEALAGRFTFTPSGAPAARDFTVAI
                     EDARQIDNGLFATFDRPLASKASLSVGVRGDRVETENQGGFFGDRSTEHSALSGHAAV
                     SVGPFSGVTATFQVARGFRDPTLSDRYFRGPSGRGFVIGNPDLDPERSLQYDASVLWA
                     VGRGSVGLYGYLYQIDDLVERYRPVNDFLFRNRGEAEIRGIELEAQTPLVAGFSLELA
                     AALARGEALDDDSPLADIAPPNVAATVRWAGERAFAFLRGAAFGEDDRPGPSEVARPG
                     YTTVDLGTGWRFTEELELRVTGRNLTDRRYPDSADEVASLAPGRTFSLGVVGRY"
     gene            complement(3444..>5420)
                     /locus_tag="VEL74_07260"
     CDS             complement(3444..>5420)
                     /locus_tag="VEL74_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HYG62362.1"
                     /translation="RAWTGVALYWGCGAILAMLPWLSRRLAQLDPSSLRTRLALALAT
                     TSSVALVLTAAVATSQEERLAVEQVIESRQIEANALARNIGDYIRLNAARTFALAAAS
                     GRAPWRPESQRRLLVATHEAYPDVTATFVLQPAGRLLVAEGRTVPLPAGMLRGLAADV
                     RLQARSDIGVRLIPAGRTVLLTFTSPILGPRAELRGVLVTAFDSEALAQRIARPSSSV
                     FLNDGHGLEIARSEISLQDLIELVGTNAQAERPAPLPPEWDLRARREGVLSSRQRLAA
                     FAIVDDLGWVVAVESPPATALDGVRRGRDLAFVLLLLVVPLAVTLGIFMARRIARPLR
                     TLSAAAAALAAGDRQVPLGDSNITEVSRLSASFAEMRDRLAERTRESERLATELRARA
                     EALADSDRRKDEFLAMLAHELRNPLGAIANAAYVLDRIGDSDAPAARSVAVIQRQIRH
                     LVRLVDDLLDVSRITRGKVELRREPVDFVQVVKHAIEMTRPVVDAREHELRLALPATP
                     LPLHADATRLEQVVGNLLRNAAKYTEPGGVIEVEVHQEEGAAAVLSVRDNGMGISADL
                     LPRVFDLFTQGEQDLDRAGAGLGIGLTLVRSLVEMHGGRVEAMSEGKDSGSRFVVRLP
                     LAETADAAAQYLPTTPSEKVLPGASEATSSAESG"
BASE COUNT          662 a         2015 c         1979 g          764 t
ORIGIN      
        1 ctgcgccgcg gcgcgggacg gatccccggc accgcgctcc cctgggagga gggcaacgtc
       61 ggcgtcgccg cccaccgcga ccgcttcttc cgtggcctga aggatatcca gaaggacgac
      121 ctcgtcacgc tgaccaccgt ccacggcacc tatcaatacc gggtggagtc gacggaggtg
      181 gtcacgccgc gcgatacgca cgtgctcgcc gataccgggg aggacacgct gaccctggtc
      241 acctgctacc ccttctacta cgtcggctcg gccccgaagc ggttcatcgt ccgtgcgcag
      301 cggatcgaag ggggagagac ggccggcggg gtgagctgag cccctgactc ctcgtccccc
      361 tcgcccccgc gcccaggagg cgctacagta gcttcatgcc tcgccgtcga tcgtggcggc
      421 cccggtggct gtccgtcccg gggctgaccg gctttctcct ggctctcctg ctggtgggag
      481 ccgtgccggg gtccgcctcg gagcccccgc tcgcccggtt gctgtccccc gcgatgggag
      541 acgagctggc ggtggggtcc atggccaccg tggagtggga gccggcggcc ggcctggccg
      601 ccatggagcg cgcggaggag tgggaggcct tcctctccgt cgacggcggc gccacctacc
      661 cgctgcgtat caccccccac ctcgacctgt cgatccggcg cttctccttc cgggtgccgg
      721 acctgcccag ccgccaagcg cggctgctgc tccgcttcgg cgatgagcgc cgggaggagg
      781 tggactgcga gacgcggcag atcttcgcca tccaccggcc gccgggctac gtgcccttgc
      841 attcacccaa gatcgccttt acccggggtg aagcggcgcg ggccggcgac gccggcgtcg
      901 tcgcctgggt ggagggcgcg cgcgacggca gcggcctgcg cgaggtggtg tcggtggccg
      961 atccgggggc ctcgctggag agcgcccggc cggccgggcc cctgctcctc cctctgctct
     1021 ggcccgcccc ggtccgcgcc gcgctggcgc ctcctgcggt ggcgccagcc gggtcctatc
     1081 ccgaaggccg cgcgatcccc gaaccctctc cggcgccggc gggagcctcc gctccggtcc
     1141 gcctgttgat ccatcggttc aacgaataga ccgagacctc gaagccccta tccccctcgc
     1201 aatgcgggcc cggcgtgggc ccgcgggaca tcagggtcgc cgtcccctcg cgcgcgccag
     1261 ctcgtgtgag ggcggctttg cattggcttt ttgaggccat cgaggtctgt catgcgtaag
     1321 ctccagggag ccccgccggg gctgccgttc gcctttcttc tcttcgtcct gacgctcgtg
     1381 ccgtcgctcg cggcggcgtt cgaagggcgt ctcctcctgc ccgacggcac cccggccgcc
     1441 ggctatcaag tctccgtcgt cggccgctcc atctcggtgc cggtgggccg cgacgggagg
     1501 ttccggatcg accccgaccc gtcgctgccg ttccggctgg tcgccatcag cccctccggc
     1561 gagctgtcgc cgccgctgga cgtcgaggcc ctgcccgagg aggtgctcga cctgacgctg
     1621 ccgccggcct tccgggagag cgtcaccgtg gcctcgggcg tggcgcccgg catcgactcg
     1681 ccccccgccg cggcgacggt gatgatcgcc caggaggatc tggagcagcg ccgccccgac
     1741 cgcctcatcg aggcgctgga gggggtggcc ggggtctcca gcaccgacca gaccacgacc
     1801 ggggtgccgg tgatccgcgg tctggccagg ggccgtaccc tgatcctcct cgacggcggc
     1861 cgggtcaccg cggagcggcg cgccgggccg tccgccagct tcctcgaccc gttcaccctc
     1921 gggtcggtcg aggtctcgcg cggcccgggc tccgtggcct acggctccga cgccttcggc
     1981 ggcgtgatca acgcccgctc gcgcttcccc gagccggggc agggcagggc gctccgcttc
     2041 cagctcgaca agggcttcgg cggcagcgac gaggagtcgg tggggctcga aggatcgacc
     2101 gacctctggg gcggcgccct gctcggccag gtcacctggc gccagggcga cgacggcgag
     2161 gcgggcggcg gcgagaccat cgacctgtcg tccttcgagg accgcagcgg cgccctgcgc
     2221 tactcccggc agacccgcct cggctacctg cgcgccggct tctccgccgc cgacgccgag
     2281 gacgtgggga agcccgcctc ggactccgac atcacccgga cgatctaccc caaggagacc
     2341 tcgcgccgct tccacctctc cctggacacc ggcccgctcg gcggctggga ctccctggag
     2401 atggggctct tcttcggaag ctaccggctg gtgctcgacc gcgaccgcct cgccacggcc
     2461 acctcgaccc ggctgatcga gcgctccgac gtcgactcga acgacggctc ggtgcgggcg
     2521 gtggcgtcgc gcggcgtgct gggcggccgt ctgcagctcg gcttcgagtc cgcctcccgc
     2581 ttcggcctcg aggcgctcgc cggccgtttc accttcaccc cctccggcgc gccggcggcc
     2641 cgggacttca ccgtcgccat cgaggacgcc cggcagatcg acaacggcct gttcgccacc
     2701 ttcgaccgcc ctctcgcctc caaggcgtcg ttgtcggtgg gagtgcgggg ggaccgggtg
     2761 gagaccgaga accagggcgg tttcttcggc gaccgctcga ccgagcactc cgccctgtcg
     2821 ggccacgcgg cggtcagcgt cggccccttc tcgggcgtca ccgcgacctt ccaggtggcg
     2881 cgcggcttcc gcgaccccac gctgtcggac cgctacttcc ggggcccgag cggccgcggc
     2941 ttcgtcatcg gcaaccccga cctcgatccg gagaggagcc tgcaatacga cgcctcggtg
     3001 ctgtgggcgg tggggagggg ctcggtcggc ctgtacgggt acctctatca gatcgacgac
     3061 ctggtggagc gctaccggcc ggtgaacgac ttcctcttcc gcaaccgcgg cgaggcggag
     3121 atccggggga tcgagctgga ggcccagacg cccctggtcg ccggcttctc cctggagctg
     3181 gcggcggcgc tcgcccgcgg tgaggccctg gacgacgact cgcccctggc cgacatcgcg
     3241 cctcccaatg tcgcggccac cgtgcgctgg gccggcgagc gcgccttcgc cttcctgcgc
     3301 ggcgccgcgt tcggcgagga cgaccgcccc ggcccttccg aggtggcgcg ccccggctac
     3361 accacagtag acctggggac gggctggcgc ttcaccgagg agctggagct gcgggtcacc
     3421 ggccgcaacc tgacggaccg gcgctacccc gactcggcgg acgaggtggc ctcgctcgcg
     3481 cccggcagga ccttctcgct gggggtggtg gggaggtact gagccgcggc gtccgcagtc
     3541 tccgccagtg ggagccggac cacgaaccgg ctcccactgt ccttcccctc gctcatcgcc
     3601 tccacccgtc cgccgtgcat ctccaccagg ctgcgcacca gcgtcagccc gatgccgagg
     3661 ccggcgccgg cgcggtccag atcctgctcg ccctgggtga agagatcgaa cacccgcggc
     3721 agcaggtcgg ccgatatgcc catgccgttg tcgcgcacgg agagcaccgc cgccgccccc
     3781 tcctcctgat gcacctccac ttcgatgacc cccccgggct ccgtatactt ggccgcgttg
     3841 cgcaggaggt tgcccaccac ctgctcgagc cgggtggcgt cggcgtggag cggcagcggc
     3901 gtggcgggca gggcgagccg cagctcgtgc tcgcgggcgt cgaccaccgg gcgcgtcatc
     3961 tcgatggcgt gcttcacgac ctggacgaag tccaccggct cgcgccgcag ctccaccttg
     4021 ccgcgggtga tgcgggagac gtccaggagg tcgtccacga ggcggaccag gtggcggatc
     4081 tggcgctgga tcacggccac cgaccgggcg gcgggcgcgt cggagtcgcc gatgcggtcc
     4141 agcacgtagg cggcgttggc gatcgccccc agggggttgc gcagctcgtg ggcgagcatg
     4201 gcgaggaact cgtccttgcg gcggtcggag tcggcgaggg cctcggcgcg ggcgcgcagc
     4261 tcggtggcca gccgctcgct ctcgcgggtg cgctccgcca ggcggtcccg catctcggcg
     4321 aacgaggccg agagacgcga gacctcggtg atgttgctgt cgcccagcgg cacctgccgg
     4381 tcgccggcgg cgagggcggc ggcggcggcg gagagggtgc gcagcgggcg ggcgatgcgc
     4441 cgcgccatga agatgcccag ggtgacggcg aggggaacca ccagcagcag caggacgaag
     4501 gcgaggtcgc ggccgcggcg gacgccgtcc agggcggtgg cgggggggct ctccaccgcc
     4561 accacccagc cgagatcgtc cacgatggcg aaggcggcga ggcgctgccg gctggacagc
     4621 accccctcgc gccgggcccg caggtcccat tcagggggga ggggggcggg cctctccgcc
     4681 tgggcattgg tgcccaccag ctcgatcagg tcctggaggg agatctcgct ccgggcgatc
     4741 tcgaggccgt ggccgtcgtt gaggaagacg ctggagctcg gccgggcgat gcgctgtgcc
     4801 agcgcctccg agtcgaaggc ggtcaccagc acgccgcgca gctcggcccg tgggcccagg
     4861 atgggagagg tgaaggtgag gagcacggtc cgtcccgcgg ggatcaggcg caccccgatg
     4921 tcactccggg cctggagccg cacgtccgcc gccaggccgc ggagcatccc cgccggcagc
     4981 ggcaccgtcc tcccctcggc cacgagcagc cgtcccgcgg gctggaggac gaaggtggcc
     5041 gtgacgtccg ggtaggcctc gtgggtggcg acgagcagcc ggcgctggct ctccggcctc
     5101 cagggggcgc ggccggaggc ggcggccagg gcgaaggtgc gggccgcgtt cagccggatg
     5161 tagtcgccga tgttgcgggc gagggcgttg gcctcgatct gccgcgactc gatcacctgc
     5221 tccaccgcca gccgctcctc ctgggaggtg gccacggccg cggtcaggac gagggcgacc
     5281 gacgaggtgg tggcgagggc cagcgccagc cgtgtccgca gggaagaggg gtcgagctgg
     5341 gccaggcgcc gagacagcca ggggagcatc gccaggatcg cgccgcaccc ccagtacagc
     5401 gcgacgccgg tccaggcccg
//
LOCUS       DASPJI010000275         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_416930, whole genome shotgun sequence.
ACCESSION   DASPJI010000275 DASPJI010000000
VERSION     DASPJI010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416930"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1660
                     /locus_tag="VEL74_07265"
     CDS             <1..1660
                     /locus_tag="VEL74_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG62363.1"
                     /translation="FELGGHSLLATRVIARIRQVLEVDLPLRAFFEHPTLVGLARAVE
                     RERSAASGLSIPPLARRQDAGPAPLSYAQQRLWVMDRLVPGSPFYNLTTAVRLSGDLD
                     ITVFRRTVNEIVRRHEVLRTAFLESDQGPRQLVLPAAPLGIPLVDLQALGEEERRLEA
                     RRLGSEEALAAFDLTAGWLIRCRLLRLRDRDHVILCTMHHIVSDYLSLGVFVEEVGIL
                     YAAFFRGEASPLPEPPVQYADFAAWQRQWLIGEVLDSEIAYWTRQLAGAPTRIALRAD
                     RSASMEPDFRGATRTFELPAPLVADLRRVSRAEGGTLFMTLLAAYGILLSHLSGQDDL
                     VVGTPIGYRNWPEIESSIGLFANTLALRCGLGGNPTFGELLARVRQTVLGAFAHQHVP
                     FERLVEELRLERSLSDNPLFQVTFNLVETAAAPPPAEAGSRGEASGVALRPFEIDRET
                     AQFDLALVLTGGGGDFTGSLQYKTALFDASTMGWLLEQFRRLLERVAVDPAVNLYELR
                     QMLVQADEERRAATDQALEEASFRKFKERRRRTVPSTLGTSGEV"
     gene            1664..>2053
                     /locus_tag="VEL74_07270"
     CDS             1664..>2053
                     /locus_tag="VEL74_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG62364.1"
                     /translation="MAQEVLSGFRLSPQQQHLWSLGAGREEASYRALCGVRVEGVLDT
                     RELERALAHLIERHEILRTSFPLLPGLERPLQVIAETAPPVLERHDFTNLAPEEQESR
                     ISGLLHEMSLRPLPKEGEPALSAALLML"
BASE COUNT          293 a          695 c          712 g          353 t
ORIGIN      
        1 cttcgagctg ggcgggcact cgctgctggc cacccgggtc attgcccgta tccgtcaggt
       61 gctggaggtg gacctgccgt tgcgcgcgtt cttcgagcac cctacactcg tgggcctggc
      121 acgggcggtg gagcgggaga ggagcgcggc cagcgggttg tcgatccccc cgctcgcgcg
      181 acggcaggac gctgggccgg cgcctctctc ctacgcgcag cagcggctct gggtcatgga
      241 ccgtctcgtg ccggggagcc cgttctacaa tctcaccacg gccgtgcggc tcagcggcga
      301 cttggacatc accgtcttcc gccggaccgt caacgagatc gtccgccgcc atgaagtgct
      361 gcggaccgcg ttcctggaga gcgatcaggg gccccggcaa ctcgtcctcc cggccgcgcc
      421 gctcggcatc cctctggtgg acctccaggc tctgggcgag gaggagagga ggctggaggc
      481 gcggcgcttg ggctcggagg aggcgctcgc cgccttcgac ctcacggcgg gatggctgat
      541 ccgctgccgg ctgctgcgtc tgcgcgaccg cgatcacgtg atcctctgca ccatgcacca
      601 catcgtctcg gactacttgt cactcggcgt cttcgtcgaa gaggtgggga tcctctacgc
      661 cgccttcttc cgcggcgagg cctcgccgct ccccgagccg ccagtccagt acgccgattt
      721 cgcggcctgg cagcggcagt ggttgatagg agaggtcctg gactccgaga tcgcctactg
      781 gacacggcag ctcgccggcg cgccgacccg gatcgccctc cgcgccgacc gctctgcttc
      841 gatggagccc gacttccggg gcgcgacgcg cacgttcgag ctgccggccc ccctggtcgc
      901 cgatctccgc cgcgtcagcc gcgccgaggg ggggacgctg ttcatgaccc tcctcgccgc
      961 ctacgggatc cttctcagtc atctctcggg acaggacgat ctggtggtgg ggaccccgat
     1021 cggctaccgg aactggccgg agatcgagag ctccatcggc ctcttcgcca acaccctggc
     1081 cctgcgctgc ggcttggggg gcaatcctac attcggcgag ctgctggcgc gggtgcgcca
     1141 aacggtgctc ggcgccttcg cccatcagca cgtgccgttc gagcggctgg tggaggagct
     1201 gcggctggag aggagtctga gcgacaaccc actgttccag gtgacgttca atctcgttga
     1261 gacggccgcc gcgccgccgc cagcggaggc cgggagcagg ggagaggcct ccggcgtcgc
     1321 gctccgtccc ttcgagatcg accgtgagac cgcgcagttc gatctcgcgc tggtgctcac
     1381 cggcggaggg ggcgacttca caggctccct gcagtacaag acggccctct tcgatgcgag
     1441 caccatgggt tggctgctgg agcagttccg gcgccttctg gagcgggtgg cggtggaccc
     1501 ggcggtgaat ctctacgagc tgcggcagat gctcgtccaa gccgacgagg agcgccgggc
     1561 ggccaccgat caggcgctgg aagaggccag cttccggaag ttcaaggagc gcagacggcg
     1621 gacggtgccg tctacgctcg ggacgtcagg cgaggtctga tccatggcgc aggaagtcct
     1681 cagcgggttc cggctctctc cacagcagca gcacctgtgg agcctcggcg ctggccggga
     1741 agaggcgtcg taccgcgctc tctgcggtgt tcgtgtggag ggggtgctgg acacgcggga
     1801 gttggagaga gcgctcgctc atctgatcga gcgccacgaa atcctgcgca cctcctttcc
     1861 tctgcttcca gggttggaac ggccactcca ggtgattgcg gagaccgcgc cccctgtcct
     1921 ggagcgccac gatttcacca acctggcgcc ggaagagcag gagtcgagga tcagcgggtt
     1981 gctccatgag atgagtctcc gtcccttgcc gaaggaagga gagcctgctc tttccgccgc
     2041 cctcctgatg ctg
//
LOCUS       DASPJI010000276        10192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_76281, whole genome shotgun sequence.
ACCESSION   DASPJI010000276 DASPJI010000000
VERSION     DASPJI010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76281"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="VEL74_07275"
     CDS             <1..1322
                     /locus_tag="VEL74_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HYG62365.1"
                     /translation="FEKKKPENASLLDSRIEESQFTWLGGNVDFVTGSGGAPGAPVVD
                     APNLARTALVESGGIKVGIFGVVIPTAAVQYVQTFEDPVETARTLSADLRARGAEVVI
                     GVTHLNARDDRRLLETLGSAGPDLILGGHDHERMACSAGGRQALKADADIRTATVARL
                     TLQPGGRVDVCWEFPKLASGSPAPDPAVSARVHNWLERHEKLFCDQAKAPADCLSEEY
                     TWTKTVLEAEESKIRGQETSLGDWIADRMVDAFVTCGAQASFINAGTLRLNQDLQAGA
                     RITRRTVEELFGFPAPLHLLRIDGRTLQQVAEHAIVGWPGSGNWLQVSGISYVHDTEA
                     GTIRDLRLTNGGVSRPVQPDDTVMVVTNDYLVNPSGDQDGYTMLNQKQIVDACAADGT
                     DLKQLVIEALEVAGEDGIEPELAGRIKQVGEAAQPPCSKEERPCGGS"
     gene            1343..4315
                     /locus_tag="VEL74_07280"
     CDS             1343..4315
                     /locus_tag="VEL74_07280"
                     /inference="COORDINATES: protein motif:HMM:NF019313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HYG62366.1"
                     /translation="MADRPLVFICFAYPDRAWKERLVSHLKTFQVESWDGERLPPGET
                     LEQVIARAAISVVLVSVYSLGSRSVNYEVGESRRHGITLLPVYLTVVPRGERSFVSDL
                     RLEGLPRSGRPLDSYGVDEVEAELARIAREIADAALEVSATPLPSSKSSTTEVASPEL
                     DDDVEASFESSSVPEPAIEEPSSLVVFPYDEEPPAPRTFLSAYAADDSRGEDLLGITK
                     DVHACASLIAARTVAPPLSIGLFGDWGSGKTFFMRQLRRRVDKIAREARESGWMQKDI
                     AYYKRIVQIEFNAWHYVEGNLWASLVEHIFDNLRIGDDRSTVATLQKHFLEKLQFEKA
                     AREDASAREKTASQNLEQAGIDLQAALEEYDRKALELAELSKENILLDLKLPTVKAQI
                     DQTLKDLGLGEVRDSVRDLRQALAEAQAVAERGVGILTPLIKAPDKNRRFVVLVVILV
                     VAPLVGLAAEPLLRWISPQLPAQLGAVATSMAALLSLGAGWIRSQAAWMSDKVGRLEA
                     ARGELDKQVEEAQAENRGRITDLRQQLEILKAEHSAAQRKREEAERRVADAQAELEQA
                     NAGWLLAKFIQDRAESSDYRKHLGVLALVRDDFETLSGLIEEENWRLAPASPNDGDLR
                     HTHPKYATLEEEDAERETRINRIVLYIDDLDRCPPKKVVEVLQAVHLLLAFPLFVVVV
                     GVDARWVARSLQKHYPDLLQDGQGKGGRAAATPDDYLEKIFQIPFWLRPMKDESCLQM
                     VQGLLAPSLEKKASDPMPKVDNSGRAPLPAPPSPDTESEGATLPAAAAPPPPMEADGA
                     PPGDELSDALGREPEEIDLAPCSLVISETEAKFIKLLAPLLGRSPRALKRFVNVYQLI
                     KAGLSEEEQATFLEERTGETADYQAVLFLLSVVTGVPEVAPDLWRVLRQAGRDYLLSD
                     SDLRWVMDEMDTAMGNDPGWRKVSSWLLPPAQSQLRLDKELKPLIHWEPEVSRYSFRV
                     APEAHPPNSPPLQPSFPS"
     gene            4613..4738
                     /locus_tag="VEL74_07285"
     CDS             4613..4738
                     /locus_tag="VEL74_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62367.1"
                     /translation="MSWWSASTSIRPLPEWRRWSVVRIVEDRDPSSATGVTFLEV"
     gene            complement(5166..5576)
                     /locus_tag="VEL74_07290"
     CDS             complement(5166..5576)
                     /locus_tag="VEL74_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYG62368.1"
                     /translation="MRAVLIDAGPLVALLDRSDGHHEEIVELTRNVKDPLVSVWPVIV
                     EAMYLLSFSWKAQKALWEILETGTVHLLPLGEEDIPSLKSLMEKYQDLPMDMADAALV
                     RVAEREGIRRVMTLDQKDFRVYRLARKGHFTLLP"
     gene            complement(5557..5817)
                     /locus_tag="VEL74_07295"
     CDS             complement(5557..5817)
                     /locus_tag="VEL74_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62369.1"
                     /translation="MATVRLDMKTEVALKRLSVKRGQTQSEVIRDAITRLAEEEGESL
                     SAFQKLQPFIGVADSGGRQLSERTGQRFREMLEERKRARRPD"
     gene            complement(5865..7049)
                     /locus_tag="VEL74_07300"
     CDS             complement(5865..7049)
                     /locus_tag="VEL74_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYG62370.1"
                     /translation="MSDHVLVAGGGIGGLTAAIALRRAGFGVRLFERAPEIREVGAGI
                     NIQSNAVLALRHISLGDAGLDEAVIAGGRLMGWASLRTTDDRVISREDMAAVGRAVGA
                     PGVAIHRATLQHLLVDALGPGVLETGREVTGYTARPDGVTVRFRDGGTADGALLVGAD
                     GIRSAVRAQLLGDGEPLYSGYVAWRGVTPAGGAVPEETVETWGPGRRFGIVPIDHGRV
                     YWYATLNTPPGGHDDPGRHRETLLRLFAGWHAPIPALLEATPEEAILRNDIVHRLPVE
                     KWGEGRVTLLGDAAHPMTPNLGQGACQAIEDAVVLAHCLRENTGAPTAALRHYEARRI
                     PRANGFVLASMRLGRLGQWENRAACWLRDRALGLVPAAAARRQLVRSMRFELWESASA
                     AG"
     gene            complement(7093..7293)
                     /locus_tag="VEL74_07305"
     CDS             complement(7093..7293)
                     /locus_tag="VEL74_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62371.1"
                     /translation="MRKKLLFLSLALALMAGAQALTPATAEAATCGLVCPPNPNGECV
                     CCEWCCTLSGGGVTCSDRPCYC"
     gene            complement(7313..7519)
                     /locus_tag="VEL74_07310"
     CDS             complement(7313..7519)
                     /locus_tag="VEL74_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62372.1"
                     /translation="MKKKLTFLVLALTLLAGAQLGLFTPATVAADTCQTICGPGPDGC
                     ECCSTCCTTSGFPGMICSPGACIC"
     gene            7654..8412
                     /locus_tag="VEL74_07315"
     CDS             7654..8412
                     /locus_tag="VEL74_07315"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYG62373.1"
                     /translation="MTTPPSNRPRALQLLVAPALVLLLLGCAPQPAAQQEAAPSAPMP
                     GNAVREAPSPAPAASVRKPANVMSHEGAAWLEREGREEAERPDIVIAAMDLRPGLTVA
                     EIGAGTGWYTRRIAPAVAPGKVYAVDIQPEMLELLKTKYSPGIPNIVTVLGTDTDPRL
                     PAGSVDRMLMVDVYHEFQKPREMLASLRKALAPGGTITVVEYRLEGETASHIYVDHRM
                     SVEQVLAEWTPAGFELVERIETLPSQHLFVFRAR"
     gene            8576..>10192
                     /gene="speA"
                     /locus_tag="VEL74_07320"
     CDS             8576..>10192
                     /gene="speA"
                     /locus_tag="VEL74_07320"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HYG62374.1"
                     /translation="MAATASLSNRWTPADSVDLYNVRGWGNTYFGVNNLGNVVVHPGG
                     PGTPAIDLKELVDEVRERGIGLPLLIRFSEIIKARVVELNEAFNRAMAEYGYRSTYRG
                     VYPIKVNQDRYLVERLVEYGRPYHYGLEAGSKPELLAVMAMLEDDEALIICNGYKDEE
                     YVETALLASKLGRHVILVVEKPSELPLIHEMSQRMKVRPHIGIRSRLSSRGAGHWEAS
                     GGDRSKFGLSGRDLLNAVEYLRANNLLDTFELLHFHLGSQISSIRSVKEGLREAAQIY
                     VNLAKMGAPLGYIDVGGGLGIDYDGSQTNFASSLNYTLQEYANDIVFGVMEVCDANDV
                     PHPTIVSESGRATVAHHAVLVMEVLGASEFEVGKLPKRPPADAEPSLRNLFETYREVS
                     RKNVLESYHDAVTYRDELLTLFNLGHLSLQDRGLAEDLFWAICQKILKITRSMPQVPE
                     ELEGLERQLADTYFCNFSVFQSLPDSWAIEQLFPILPIHRLDEEPTDRAVLADITCDS
                     DGKIDHFIDRRDVKDILELHPLVSGAPYYLG"
BASE COUNT         1711 a         3462 c         3388 g         1631 t
ORIGIN      
        1 agttcgagaa gaagaagccg gagaacgcct cgctgctgga cagccgcatc gaggagtcgc
       61 agttcacctg gctggggggt aacgtcgatt tcgtgaccgg ctccggcggc gcgccgggcg
      121 ctccggtagt ggacgccccg aacctggcgc gcaccgcgtt ggtggagagc ggcggcatca
      181 aggtcggcat cttcggcgtg gtcatcccca ccgcggcggt gcagtacgta cagaccttcg
      241 aggatccggt ggagacggcg cgcaccctct cggccgacct gcgggcgcgc ggcgccgagg
      301 tggtgatcgg cgtcacccat ctgaacgcgc gggacgaccg gcggctgctg gagacgctgg
      361 gcagcgccgg cccggacctg atcctcggcg gccatgacca cgagcgcatg gcgtgcagcg
      421 ccggcggccg tcaggcgctc aaggcggacg ccgacatccg caccgccacg gtggcgcggc
      481 tgaccctcca gcctggcggc cgggtggacg tctgctggga gttcccgaag ctggcctccg
      541 gctcgccggc gccggatccg gcggtgtcgg cgcgcgtcca caattggctg gaacggcacg
      601 agaagctctt ctgtgaccag gccaaggcgc cggccgattg cctgagcgag gagtacacct
      661 ggacgaagac ggtcctggag gccgaggaga gcaagatccg cggccaggag acctcgctgg
      721 gcgactggat cgccgaccgc atggtcgacg ccttcgtcac ctgcggcgcc caggcgtcct
      781 tcatcaacgc cggcacgctg cgcctgaacc aggatctcca ggccggcgcg cggatcaccc
      841 ggcgcacggt ggaggagctc ttcggcttcc cggcgccgct ccatctgctg cgcatcgacg
      901 gccggacgct ccagcaggtg gcggagcacg cgatcgtcgg ctggcccggc tccggcaact
      961 ggctccaggt ctccggcatc agctacgtgc acgacaccga ggccggcacc atccgcgacc
     1021 tccggctcac caacggcggc gtctcgcgtc cggtccagcc cgacgatacg gtcatggtcg
     1081 tcaccaatga ttacctggtg aacccctccg gcgaccagga cggttacacg atgctgaacc
     1141 agaagcagat cgtggacgcc tgcgcggcgg acggcacgga cctgaagcag ttggtcatcg
     1201 aagcgctgga ggtggccggg gaggacggca tcgagccgga gctggcaggg cggatcaagc
     1261 aggttgggga ggcggcgcag ccgccgtgca gcaaggagga gcggccttgc ggggggagtt
     1321 agatcgctag agggaggctc tgatggcgga cagaccgctg gtcttcatct gctttgctta
     1381 cccagaccgc gcgtggaagg agcgactcgt cagccacttg aagacgtttc aagtggaatc
     1441 ctgggacggg gaacgactgc cgccgggtga gactttggag caagtcattg ctcgggctgc
     1501 gatctctgtc gttttggtta gcgtgtactc tctcggatct cgctcagtca actacgaagt
     1561 aggagaaagt cggaggcacg gcatcacatt gcttcccgtt tatctcaccg tggtcccacg
     1621 aggtgagagg agttttgtga gcgatcttcg acttgagggt ctgccgagga gcggtcgtcc
     1681 cctggattcg tacggagtcg atgaagtcga ggccgagctg gcgagaatcg cgcgagagat
     1741 cgctgatgcc gctttggaag tctctgcgac ccctctgccc agctcgaaat cctctaccac
     1801 cgaagtagcc tcgccggaac ttgacgacga tgtcgaagct tccttcgagt cctcctccgt
     1861 gccggaaccg gctatcgagg agccctcctc actcgtcgtc tttccatacg atgaagagcc
     1921 ccctgccccc cgcaccttcc tctccgccta cgccgccgat gactcccgag gagaggacct
     1981 cctgggcatc accaaggacg tccacgcctg cgcctcgctc atcgcggccc gcacggtcgc
     2041 gcctcctctc tccatcggtc tcttcggcga ctggggatcg ggcaagacgt tcttcatgcg
     2101 ccagctccgg cggcgggtgg acaagatcgc ccgcgaggcc cgcgaatcgg ggtggatgca
     2161 gaaggacatc gcctattaca agcgcatcgt ccagatcgag ttcaacgcct ggcattacgt
     2221 ggagggcaac ctctgggcga gcctggttga gcacatcttc gacaacctgc ggatcggtga
     2281 cgaccggagc accgttgcga cgcttcagaa acacttcttg gagaagctcc agttcgagaa
     2341 ggccgcgcga gaggatgcct ccgcccgcga aaagaccgcc agccagaacc tggagcaggc
     2401 cggtatcgat ttgcaggctg ccctggaaga gtacgaccgt aaggccctcg agctggcgga
     2461 gctgtccaag gagaacatcc tcctggatct aaagctcccc acggtcaagg cgcagatcga
     2521 tcagaccttg aaggatctgg ggctgggaga ggtgagggac tccgtccggg atctacgaca
     2581 ggctcttgcc gaggctcagg ctgtcgccga gcggggagtt ggtatcctga ctccgctcat
     2641 caaggcgcca gacaagaacc gccgatttgt cgtcctggtc gtgatcctcg tggtggcccc
     2701 cctcgtggga ttggcggccg agccactcct gcgctggatc tctccgcagc tgcccgccca
     2761 actgggggcg gtcgccacca gcatggcggc gcttctctcg ctgggtgcgg gctggatccg
     2821 gagccaggcc gcatggatgt ctgacaaggt aggccggctc gaagcggcgc gcggcgagtt
     2881 ggacaagcag gtggaggagg cccaggccga gaaccggggc cggatcacgg acctgagaca
     2941 gcagcttgag atcctcaagg ccgagcactc cgctgcccaa cgcaagcgtg aggaggcgga
     3001 gcggcgtgtc gcagatgccc aggctgagct ggagcaggcc aacgcgggtt ggctgctcgc
     3061 caaatttatc caggacaggg ccgagagcag cgactaccgc aagcacctgg gcgtgctcgc
     3121 cctcgtccgt gacgacttcg aaactttgtc aggtctgatc gaggaggaga actggcgcct
     3181 cgccccggca tctccgaacg acggtgacct ccggcatacc catcccaagt acgccaccct
     3241 cgaggaggag gacgcggaac gggagacccg catcaaccgc atcgtccttt atatagatga
     3301 tcttgaccgc tgcccgccga aaaaggtcgt ggaggttctc caggccgtcc acctgctgct
     3361 cgcgttcccg ctgttcgtgg tcgtggtggg ggttgatgcc cgttgggtgg cgcgctcgct
     3421 ccagaagcac tatccggatc tgttgcagga tgggcagggc aagggaggta gggccgcggc
     3481 gactcctgat gactacctgg agaagatctt ccagatccct ttctggctgc ggcccatgaa
     3541 agacgagtcc tgtctccaga tggtccaagg actgctcgca ccgagcctcg agaagaaggc
     3601 atcggatccc atgccgaagg tcgacaacag cggtagggct cccctccctg cgccgccctc
     3661 tccggatacg gaaagcgagg gcgcgactct ccccgccgca gccgcccctc cgcctcccat
     3721 ggaagcggac ggtgcgccac ccggagatga attgtcagac gctttgggca gagagccgga
     3781 ggagatcgac ctcgctccgt gcagcttggt gatcagcgag accgaagcga agttcatcaa
     3841 gctgcttgca ccacttctcg gccgctctcc ccgcgctctc aagcggttcg tcaacgtcta
     3901 ccaactcatc aaggcggggc tgtccgagga ggagcaggcg acttttctgg aggagaggac
     3961 gggagagaca gcggactacc aagctgttct cttcctcctg tctgtggtga cgggcgtacc
     4021 cgaggtcgca ccggacctct ggcgggtcct ccgccaagcc ggtcgagact atcttctaag
     4081 cgactcggat ctccggtggg tcatggacga gatggacacg gctatgggaa acgatcccgg
     4141 atggagaaag gtaagttcct ggctgctccc acccgcccag agccaactcc ggctcgacaa
     4201 ggagctgaag ccgctcatcc actgggagcc cgaggtgagc cgctactcct tccgcgtagc
     4261 ccccgaggcg caccctccga attcccctcc cctgcaaccc tcatttccct cttgacgcgc
     4321 accaggagcc ggtataggct ccgagcgtcg taagcaggac agcaaaggag gtggtagggg
     4381 agagagaaga gaagccgcag tgcacagcac agccgtacag gatcgctgtg tctccgaggt
     4441 gagcgcgtag ctcatccggg atcacgaggt ccaagcggcc aagggggaga gttggcgctc
     4501 gtcccccgag gccttgccgg acaggagatg aacctcatgc ccagctacag tgatcgtacg
     4561 ccctctcgcg tcgatctgtc ccgtcccacc gaagccaccc gcagcgttct ccatgagctg
     4621 gtggtccgcg tctacttcca tccggccgct ccccgagtgg cggcgctggt cggtggtacg
     4681 gatcgtcgag gaccgggatc cgtcgagcgc gacgggcgtg acctttctgg aggtctgacg
     4741 ctccccgcac ggcggtcgcc gcctcgcgcg gcccgccgat ccatagaagg aagcagcgcc
     4801 ttttccgccg tcgcggggga ggcgctttct ctttggggaa gcttccggcg gcgcgccgga
     4861 aactctgaca gcgcaccgga agcttccggc ggattggcga agagtacgcc ggcgtgttgg
     4921 aagcttcgat ggcacgttgg aagctccggc agcgcgtcga aggctccgac agcctgccga
     4981 aggctacgac ggcgcgtcgg aagcttcggc agcgtgttgg aagcttccga cggcctgccg
     5041 aaggttacga cggcgcgtcg gagactccga cagcccgccg aaggttacga cggcgcgtcg
     5101 gaagcttcgg cagcgcgttg gaagcttccg gcggtctgtc gaagggtacg atgacgcgtc
     5161 ggaagctacg gcaggagggt gaagtgccct ttgcgggcga ggcgatagac cctgaagtcc
     5221 ttctggtcca aggtcatgac gcggcggatg ccctctcgct cggcgactcg gacgagcgcg
     5281 gcgtctgcca tgtccatggg aaggtcctga tacttttcca tcaggctttt cagagatgga
     5341 atgtcctcct cgcccagagg cagcagatgg accgttccgg tctccaggat ttcccagagc
     5401 gccttctgtg ccttccagga gaaggagagg agatacatcg cctccacgat gaccggccag
     5461 acgctgacca ggggatcctt gacgttcctc gtcagctcga cgatctcttc atgatgaccg
     5521 tcgcttcggt cgaggagcgc gaccagtggc ccggcgtcaa tcaggacggc gcgcacgctt
     5581 ccgttcctcg agcatctcgc ggaaccgctg ccctgttctc tccgagagtt gccgcccgcc
     5641 actgtccgcg acgccgatga acggctgaag tttctggaat gcggatagag actccccctc
     5701 ctcttcggcg agccgagtga tggcgtctcg gatcacctcg gattgggtct gtccccgctt
     5761 gacggacagg cgcttcaacg ccacctcagt cttcatgtcg agtcgaaccg ttgccatccc
     5821 gggcctcctg taacacacac agtgtaacac gaaacatctc ccctctaccc tgcggcggag
     5881 gcactctccc agagctcgaa cctcatcgac cgcacgagct ggcggcgggc ggcggcagcg
     5941 gggactagac cgagggcgcg gtcgcgcagc cagcaggcgg cgcggttctc ccattgcccg
     6001 agacggccga ggcgcatgga ggcgaggacg aagccgttgg cccgggggat gcgacgcgcc
     6061 tcgtagtgcc ggagggcggc ggtgggggcg ccggtgttct ctcgcaggca gtgggcgagc
     6121 accacggcat cctcgatcgc ctggcaggcg ccctgtccca ggttcggggt catggggtgc
     6181 gcggcgtccc ccagcagggt caccctccct tccccccact tctcgaccgg cagccggtgg
     6241 acgatgtcgt tgcgcaggat cgcctcctcg ggggtcgcct ccaggagcgc ggggatcggg
     6301 gcgtgccagc ccgcgaagag gcggagcagc gtctcccggt gccggccggg gtcgtcgtga
     6361 ccgccgggcg gcgtgttcag ggtggcgtac cagtagacgc gtccgtgatc gatggggacg
     6421 atgccgaagc gccgccccgg cccccaggtc tcgaccgtct cctccgggac cgctcctccc
     6481 gcgggcgtga cgccccgcca ggcgacgtag ccggagtaca gcggctcgcc gtctcccagg
     6541 agctgggcgc gcaccgcgga gcggatgccg tcggccccga cgagcagggc gccgtccgcg
     6601 gtgccgccgt cccggaagcg caccgtcacg ccgtccggcc gcgccgtgta gccggtgacc
     6661 tcgcgcccgg tctccaggac gccgggcccc agggcgtcga cgagcaggtg ctggagcgtc
     6721 gcgcggtgga tcgccacgcc gggcgctccc acggcgcggc ccaccgcggc catgtcctcg
     6781 cgcgagatca cccggtcgtc cgtcgtccgc aggctcgccc agcccatcag ccggccgccg
     6841 gcgatcaccg cttcgtccag gccggcatct ccgagggaga tgtggcgcag ggcgaggacc
     6901 gcgttgctct ggatgttgat ccccgcgccg acctcgcgga tctcgggcgc ccgctcgaag
     6961 agccggacgc cgaaaccggc gcggcgcagg gcgatggcgg ccgtcaggcc gccgatgccg
     7021 ccgccggcga cgaggacgtg gtcggacatg cgcccccaat cctaccggaa cgggggcgcc
     7081 atccccgaga gctcagcagt agcaggggcg gtcgctgcag gtgacgccgc cgccggacaa
     7141 ggtgcagcac cattcgcagc agacgcattc gccgttggga ttgggcggac agaccaggcc
     7201 gcaggtagcg gcctccgcgg tcgcgggggt gagcgcctgg gcgcccgcca tcagcgccag
     7261 ggccagggac aggaacagca gcttcttcct catggtcggg ttctcctttt cttcagcaga
     7321 tgcaagcccc cgggctgcag atcatccctg gaaagcccga ggtcgtgcag caggtgctgc
     7381 agcactcgca gccgtccggc cccggaccac agatcgtctg gcaggtgtcg gcggccacgg
     7441 tcgccggcgt gaagagcccc aactgggcgc cggccagcag cgtcagggcg aggaccagga
     7501 acgtcagctt cttcttcata ggcacctccc gggattgtgg gattgagctc cctctacaac
     7561 aagtggtgtc tgaaaggcgc ggaccggcgc ctccgggtcc gcctcgggag taccccggcg
     7621 ctccgcggtg ttagagtcgg cgaggccccc gacatgacga caccgccctc caaccgtccc
     7681 cgcgccctcc agctcctcgt cgcccccgcc ctcgtcctgc tcctcctcgg ctgtgccccg
     7741 cagccggcgg cacagcagga ggccgcgccg tccgcgccga tgcccggcaa cgccgtccgc
     7801 gaggccccgt ctcccgcacc ggccgcatcg gtgcgcaagc cggcgaacgt gatgagccac
     7861 gagggggcgg cctggctcga gcgcgaggga cgcgaggagg ccgagcggcc ggacatcgtg
     7921 atcgccgcga tggacctgcg gccggggctc accgtcgccg agatcggcgc cggcaccggc
     7981 tggtacaccc gccgcatcgc ccccgccgtc gcccccggca aggtctacgc ggtcgacatc
     8041 cagcccgaga tgctggagct tctcaagacc aagtactcgc cggggatccc caacatcgtg
     8101 accgtcctgg gcaccgacac cgacccgcgg ctgccggcgg gctcggtgga ccggatgctc
     8161 atggtcgacg tctaccacga gttccagaag cccagggaga tgctcgccag cctccgcaag
     8221 gcgctggcgc cgggggggac catcaccgtg gtcgagtacc ggctggaagg ggagaccgcc
     8281 tcgcacatct acgtcgacca ccggatgtcg gtcgagcagg tgctggccga gtggaccccc
     8341 gccggcttcg agctggtgga gcggatcgag accctcccct cgcagcacct gttcgtcttc
     8401 cgggcgcggt gaccgccagg tcccccgcgc gggaaggaca gcccccggtt tcgtgttaaa
     8461 gtccccgccg gccggagtgt gtccgccagg gcttcgtgtg tttcctcccc tccgtgccgc
     8521 cggacacgac gacaactttt ttctcccctg aggattagag gagtttccac cagagatggc
     8581 agcgaccgca tccctgtcca accgctggac ccccgccgat tccgtcgacc tgtacaacgt
     8641 ccgcgggtgg gggaacacct atttcggggt caacaacctc ggtaacgtcg tcgtccatcc
     8701 cggggggccc ggcacgccgg ccatcgacct caaggagctg gtggacgagg tgcgggagcg
     8761 cggcatcggc ctgccgctcc tcatccgttt ctccgagatc atcaaggcgc gggtggtgga
     8821 gctcaacgag gcgttcaacc gcgccatggc cgagtacggc taccgcagca cctaccgcgg
     8881 cgtctacccg atcaaggtca accaggaccg ctacctggtg gagcggctgg tcgagtacgg
     8941 gcgcccctac cactacgggc tcgaggcggg ctcgaagccg gagctcctcg ccgtcatggc
     9001 gatgctcgag gacgacgagg cgctcatcat ctgcaacggc tacaaggacg aggagtacgt
     9061 cgagaccgcg ctcctcgcct cgaagctcgg ccgccacgtc atcctggtgg tcgagaagcc
     9121 gtccgagctg ccgctgatcc acgagatgtc gcagcgcatg aaggtgcggc cgcacatcgg
     9181 catccgctcg cgcctctcct cccgcggcgc cgggcactgg gaggcctcgg gcggcgaccg
     9241 ctccaagttc ggcctgtccg ggcgcgacct gctgaacgcc gtcgaatacc tgcgcgccaa
     9301 caacctgctc gacaccttcg agctgctcca cttccatctc ggcagccaga tctcctccat
     9361 ccgctcggtc aaggaggggc tgcgcgaggc ggcgcagatc tacgtcaacc tcgccaagat
     9421 gggggcgccg ctcggctaca tcgatgtcgg cggcggcctc ggcatcgact acgacggctc
     9481 gcagaccaac ttcgcctcgt cgctcaacta caccctccag gagtacgcca acgacatcgt
     9541 cttcggcgtc atggaggtgt gcgacgccaa cgacgtgccg cacccgacga tcgtctcgga
     9601 gtccggccgc gccaccgtcg cccaccacgc ggtgctggtg atggaggtgc tcggcgcctc
     9661 ggagttcgag gtcggcaagc tccccaagcg gccgccggcg gacgccgagc cgtcgctgcg
     9721 caacctgttc gagacctacc gcgaggtgtc gcgcaagaac gtcctggagt cctaccacga
     9781 cgcggtcacc taccgggacg agctgctgac cctgttcaac ctcgggcacc tgtcgctcca
     9841 ggaccgcggc ctcgccgagg acctgttctg ggccatctgc cagaagatcc tcaagatcac
     9901 ccgcagcatg ccgcaggtgc ccgaggagct cgaagggctc gagcggcagc tcgccgatac
     9961 ctacttctgc aacttctcgg tcttccagtc gctgcccgac tcgtgggcga tcgagcagct
    10021 cttccccatc ctgccgatcc accggctgga cgaggagccg accgaccgcg ccgtcctcgc
    10081 cgacatcacc tgcgactccg atggcaagat cgaccacttc atcgaccggc gcgacgtcaa
    10141 ggacatcctc gagctccatc cgctggtgtc cggggccccc tactacctgg gt
//
LOCUS       DASPJI010000277         3063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_132175, whole genome shotgun sequence.
ACCESSION   DASPJI010000277 DASPJI010000000
VERSION     DASPJI010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132175"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VEL74_07325"
     CDS             <1..911
                     /locus_tag="VEL74_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015141368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HYG62375.1"
                     /translation="SFKGMGTDFADLNGDGMMDIFVSNIAQNWALEESHFLWMSTGRP
                     EQLRQGIAPYRDESEPLGLARSGWGWDTRLADFDNDGTHEAIQALGFIRGQRNRWPEL
                     HELAMGNDNNIQRPGMWPKFLPGDDLSGHLHNPFFVRDEDGRFFDLAADIPGLGDLSV
                     SRGLSVADVDGDGDEDFAVANQWETSYFFRNDSPRQHTALHLDLRLPAAEGRTRPAVG
                     AAATVRLPDGKKMVAQVDGGSGHSGKRAPEIHFGLGRLAAGTPLLVDVAWRDATGTVR
                     RQTLRLTPGRHTVLLGSNQKLTEKVG"
     gene            915..1829
                     /locus_tag="VEL74_07330"
     CDS             915..1829
                     /locus_tag="VEL74_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017742123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enediyne biosynthesis protein UnbU"
                     /protein_id="HYG62376.1"
                     /translation="MSEKKKDMRLAALRRFATAITILNILGHSYLGFEQSLAQPVVAL
                     LTAYSVEILLELFMAWRHGKRPRFLGPGTTFIDFLLSAHISGLAVSMLLYANEALLPI
                     AFAAAAAIGSKYLFRAGVNGSSRHFFNPSNVGITATLVAFPWVGIAQPYMFTENLGPT
                     GDWVLPAIIICTGSFLNTVFTKRVPLILSWAGAFVLQAALRNVLLDQSFLAALNPMTG
                     VAFLLFTFYMVTDPATTPNTVRGQIAFGASVALVYGLLVAIHVVFGLFFALTLVCIGR
                     GALMHARALADLRAAERVEMPVAAVAGQ"
     gene            1879..>3063
                     /locus_tag="VEL74_07335"
     CDS             1879..>3063
                     /locus_tag="VEL74_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015802826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HYG62377.1"
                     /translation="MSSQGIAIVGMACRYADARTPAELWENVLARRRAFRRIPAERLR
                     LEDYLSGRNDPDGLYSTEAAVLEGWEFDRVRFKVAGSTFRTADLTHWLALDVASAALA
                     DAGFADGEGLDRDTAGVLLGNSLTGEFSRAQLMRMRWPYVRRTLAAALEREGWEPSWV
                     AGMLERLEAEYKAPFAVPTEESLAGGLSNTIAGRICNHFDLHGGGYTVDGACASSLLA
                     VANACSALTAGDLDTALAGGVDLSLDPFELVGFARTGALALEEMRVFDARSAGFIPGE
                     GCGLLVLMREEDALRLGCRIYAVIRGWGISSDGQGGISRPEPDGQVLALRRAYRRAGF
                     DIGTIGYFEGHGTGTAVGDAAELTALSRALREAAPGAVPGPAAPVGSIKANIGHTKAA
                     AGV"
BASE COUNT          434 a         1146 c         1029 g          454 t
ORIGIN      
        1 actccttcaa gggcatgggc accgacttcg ccgacctgaa cggcgacggc atgatggaca
       61 tctttgtcag caacatcgcc cagaactggg cgctcgagga gagccacttc ctttggatga
      121 gcaccggccg gcccgagcag ctccgccagg ggatcgcccc ctatcgtgac gagagcgagc
      181 cgctgggtct cgcccgtagc ggctggggct gggacacccg cctggccgac ttcgacaacg
      241 acggcaccca cgaggcgatc caggccctcg gcttcatccg cggccagcgc aaccgctggc
      301 ccgagctgca cgagctggcg atgggcaacg acaacaacat ccagcgcccc gggatgtggc
      361 ccaagttcct gccgggggac gacctctccg gccatctgca caacccgttc ttcgtccggg
      421 acgaggacgg ccggttcttc gacctggcgg ccgacatccc cggcctcggc gacctgagcg
      481 tcagccgcgg actctccgtg gccgatgtcg acggcgacgg cgacgaggac ttcgcggtcg
      541 ccaaccagtg ggagacctcc tacttcttca ggaacgacag cccgcggcag cacaccgcgc
      601 tgcacctcga cctccggctg cccgccgccg agggccggac gcggccggcg gtcggtgccg
      661 ccgccaccgt ccgcctgccc gacggcaaga agatggtggc gcaggtggac ggcggcagcg
      721 gccactccgg caagcgggcg ccggagatcc acttcggcct cggacgcctg gcggcgggca
      781 cgccgctgct ggtggacgtg gcctggcgcg acgcgaccgg caccgtgcgc cggcagacgc
      841 tccggctgac cccgggccgt catacagtgc ttctgggatc gaatcagaaa ctgacggaga
      901 aggtgggcta gacgatgagc gagaagaaaa aggacatgcg cctggccgcg ctgcggcgct
      961 tcgccacggc catcaccatc ctgaacatcc tgggccactc ctacctcggg ttcgagcagt
     1021 ctctcgccca gcccgtggtg gcgctgctca ccgcctattc cgtcgagatc ctcctcgagc
     1081 tgttcatggc ctggcggcac ggcaagcgcc cccgcttcct gggccccggc accacgttca
     1141 tcgacttcct gctgtcggcc cacatcagcg gcctggcggt gtcgatgctc ctctatgcca
     1201 acgaggcgct gctccccatc gccttcgccg ccgccgccgc catcggctcc aagtacctgt
     1261 tccgggccgg cgtcaacggc agcagccgcc acttcttcaa cccctcgaac gtggggatca
     1321 ctgcgaccct ggtcgccttc ccctgggtgg gcatcgccca gccctatatg ttcaccgaga
     1381 acctggggcc gacaggcgac tgggtgctgc cggcgatcat catctgcacc ggctccttcc
     1441 tgaacaccgt gttcaccaag cgggtgccgc tgatcctctc ctgggccggc gccttcgtgc
     1501 tgcaggccgc cctgcgcaac gtcctcctgg accagtcgtt cctcgccgcc ctcaacccga
     1561 tgaccggggt ggccttcctg ctcttcacct tctacatggt gaccgacccg gcgacgacgc
     1621 cgaacacggt gcgggggcag atcgcctttg gcgcgtcggt ggcgctggtc tacggcctgc
     1681 tggtggcgat ccacgtggtc ttcggcctgt tcttcgccct caccctggtc tgcatcggcc
     1741 gcggcgccct gatgcacgcc cgggcgctgg cggacctgcg ggccgcggag cgggtggaga
     1801 tgcccgtggc cgcggtggct ggccagtaac gaaagggaga ctccgaacgc ccgaaccgca
     1861 ccggagagga ggatccaggt gagcagtcaa gggatcgcga tcgtcggaat ggcctgccgc
     1921 tacgccgatg cccgtacacc ggcagagctg tgggagaacg tcctggcgcg gcgccgggcc
     1981 ttccgaagga tcccggccga acggctgcgc ctggaggact acctctccgg acgcaacgac
     2041 ccggacgggc tctactcgac cgaggcggcg gtcctcgaag ggtgggagtt cgaccgggtc
     2101 cgcttcaagg tcgccggcag caccttccgc accgccgacc tcactcactg gctcgccctc
     2161 gacgtggcca gcgcggccct cgccgacgcc ggcttcgccg acggcgaggg cctcgaccgc
     2221 gacaccgcgg gcgtgctgct gggcaacagc ctcaccggcg agttctcccg cgcccagctc
     2281 atgcggatgc gctggcccta cgtgcggcgc accctggccg ccgccctgga gcgggaggga
     2341 tgggagccgt cgtgggtggc cgggatgctg gagcggctcg aggccgagta caaggcgccg
     2401 ttcgccgtcc ccaccgagga gtcgctcgcc ggcgggctct ccaacaccat cgccggccgc
     2461 atctgcaacc acttcgacct gcacggcggc ggctataccg tcgacggggc ctgcgcctcg
     2521 tcgctgctgg ccgtcgccaa cgcctgctcg gccctgaccg ccggcgacct cgacaccgcc
     2581 ctcgccggcg gcgtcgacct ctctctcgac cccttcgagc tggtgggctt cgcccgcacc
     2641 ggcgccctgg cactggagga gatgcgggtc ttcgacgccc gctcggccgg cttcatcccc
     2701 ggcgaggggt gcggcctcct ggtcctcatg cgcgaggagg acgccttgcg cctggggtgc
     2761 cggatctacg ccgtgatccg cggctggggc atctcctccg acggccaggg ggggatcagc
     2821 cggccggagc cggacgggca ggtcctggcg ctacggcgcg cctaccgccg ggccggcttc
     2881 gacatcggca ccatcggcta cttcgagggg cacggcaccg gcaccgcggt aggcgacgcc
     2941 gcggagctga ccgcgctctc ccgcgcgctc cgcgaggcgg cccccggcgc cgtcccgggg
     3001 ccagccgctc cggtgggctc gatcaaggcc aacatcggcc acaccaaggc ggccgccggg
     3061 gtg
//
LOCUS       DASPJI010000278         8074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_359266, whole genome shotgun sequence.
ACCESSION   DASPJI010000278 DASPJI010000000
VERSION     DASPJI010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8074)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359266"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VEL74_07340"
     CDS             complement(<1..644)
                     /locus_tag="VEL74_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62378.1"
                     /translation="MAHETMEHLTWRLATALRHDDGAELLHLLTCPECRDAATAVLRA
                     GDGIPGVPVPDYDPMFRRLEANLVHLEEERRRHGEAARLVAELCSLPPKEREKAIKSL
                     RFERADVLHALLREGEARQEAAPAEAVALGRLAVGLARRLSWPEAGQKAAARARALIL
                     TAGFCRLAGQESLAADLLRRAIPWLAEPRVRGDYCRTLALLRWQQGRTEEAIAL"
     gene            complement(660..2969)
                     /locus_tag="VEL74_07345"
     CDS             complement(660..2969)
                     /locus_tag="VEL74_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG62379.1"
                     /translation="MSLLDDLLIPILPPPPFPKPSREAILRELRASFAAGVGVRVISG
                     PAGIGKTHLARRLQAELAPTTRMVWARGTAWGRPFQPLREAAESLRLWPLEEPALPRE
                     LAGVPAEVRERETWISLFRKRGQDGPLLVLCCDDLDSFDAPSWECVREMLRRRERLTV
                     SLLLLTRTGPSRLALDVLSSERPVEVPPLNPEELAACCQERMGGPPWFEHAFPWLREA
                     SGGNPRHLLEILGYLRDTERLVPSAMGWRRAPESSLDVPCFEELVRRRLEAALAREKP
                     ETRSWIERGAVLGSRMEVPPAVAPALERVGERTGYLEHADERPDSFRFADQRVREAVR
                     MLCEDRRPALDEGDPRLAALLEEEGKWHQAAEETAVAASEALHEEMPSEAARLALWMD
                     RFLGRAGEAPDSPARLAAAEIRARALLGLGRYEEIVDHLGPRADGAFRAGGSGLLYLL
                     GTAARLAPPPTNYPARRWLEQARQLLGASADPAQAARIQLELALVQRGEMDERTASRI
                     FSKLQDLATKAKDVPLQLQLARRSRLFLEPGLARVNLEQALKQAGEAGLELEEALCHN
                     NLAVAFYELGLLGVAEYHARACLEVLEPYGPWGTAVPLNNLGLVAAARGEWDRAGELL
                     DRAGDAAGEVETDLWVRCNQAMVRATQGAPGVAADHLRHLAVEAGRSAPLPVRQAILF
                     NLARALLESGEAAEALAAATVPPPWLPGDEDLARDALASLRLKAVRQSRAGRPEKGLL
                     RQSRLLDLSTKPQAWIYRTLWARGDLLFL"
     gene            complement(3123..3362)
                     /locus_tag="VEL74_07350"
     CDS             complement(3123..3362)
                     /locus_tag="VEL74_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62380.1"
                     /translation="MAANPPVYRVLSSFAGCIHRGEDREAAHRAYRDAIQKVEQEGDG
                     SVALMSGSAVVERFQPRPNRPGTPWLSRFLRWLRP"
     gene            3924..5357
                     /locus_tag="VEL74_07355"
     CDS             3924..5357
                     /locus_tag="VEL74_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62381.1"
                     /translation="MAKTPAAGLVVTVCREIAGVTQAVLARFAGVHRMDIVRAEKGQG
                     RLTPGFLGRLMGFPEAVTEGLRLLVEWLRPAPEERSFPPLGLPLRERRLIAGAALNHA
                     AAQAERLAMARLADLIHADRENAKQDWLLMRAWPVEEWRGYLEEAPQVATWALVERLA
                     EESTRAAAADPQRALELAELACWVAPRIEGTREWRGRVEGWALAFYANALRVLGRFKE
                     AEAAFARAWTLWRAGASLGPGPLDESRLYDLEASLRRDLRQWPEALALLDNALALCES
                     PEGEARVLLIRAFTLEQKGDALAALAVLDRAEPLVDDEKEPRQVFGVRFNRAVNLLHL
                     GRSNEAQALLADLEERVQRLDNDFDSARLLWLRSRVDARVGDEEAAEGRLLQVRQEFA
                     RPGLEFDVALADLELARLYLRQGRNAETRELAAAAWPVFRRLGVAREAAEAIQLFWKA
                     ARRERATVDMADRALEALLRAWPCPAA"
     gene            complement(6507..6899)
                     /locus_tag="VEL74_07360"
     CDS             complement(6507..6899)
                     /locus_tag="VEL74_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62382.1"
                     /translation="MAQVHHKIELGPKQLPRLSLAIDLLGCLLQRGHGARNVPHLFQG
                     EGSIEEDMPFGRRCAACRESPIKQIQSLGKPALVTPERRLQIEEPGEVESLSTRRKES
                     RAECPCANEGRFGPLEIASHSQGISFER"
     gene            6885..7328
                     /locus_tag="VEL74_07365"
     CDS             6885..7328
                     /locus_tag="VEL74_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62383.1"
                     /translation="MNLCHLGRFQEAEKLLPSVWMLAAELENRNDLVRVSWLESRVLA
                     GKGDLEKAISTLERVREAFAERKNLYDVGRSTLELARLYLRQGRTDKTKELAARAEPV
                     FRELNVAPRALESVLVFLEAARQEAATLELLDQALQALGQGRRGR"
     gene            7371..7553
                     /locus_tag="VEL74_07370"
     CDS             7371..7553
                     /locus_tag="VEL74_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62384.1"
                     /translation="MRPSIKRLLFAGAITLTLFVPRPAQAYVICNHYDCMSVCTFYDD
                     QDNVLRKSFIPTQCSM"
     gene            7563..>8074
                     /locus_tag="VEL74_07375"
     CDS             7563..>8074
                     /locus_tag="VEL74_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62385.1"
                     /translation="MKRRDAWFFSALLFGASFLEAAEMDRALTQVRPQHLAPSSTAWS
                     GAQFVQADREGRIFLLRSEALEVYPLEADGQFGEVQRLKALPSSGPFLERVFGAVMMG
                     PGDWFLEQGGRVRLFRGGQEESLEDPRWQISGLGASGGDPVIAGTSGEAMAQVFLRKE
                     SAKPAPLMLT"
BASE COUNT         1233 a         2688 c         2869 g         1284 t
ORIGIN      
        1 aacagggcga tggcctcctc ggtccgtccc tgctgccagc gcagcagcgc cagagtccgg
       61 cagtaatccc cccgcaccct cggctccgcg agccagggga tcgcccggcg aagcaggtcc
      121 gcggcgaggg actcctgacc ggccaggcgg cagaagccgg cggtcagaat cagcgcccgg
      181 gcgcgggccg ccgccttctg gccggcctcg ggccaggaca gccgtctggc caggccgaca
      241 gcgagccggc cgagagcgac cgcttccgcg ggggctgcct cctgccgggc ctcgccctcc
      301 cgaagcaggg cgtgcagcac gtcggcgcgc tcgaagcgca ggctcttgat ggctttctcg
      361 cgctccttgg ggggcaggga gcacagctcg gcgaccagcc gcgctgcctc gccgtgccgc
      421 cgccgctcct cctccagatg gacgaggttg gcctcgagcc gccggaacat cgggtcgtag
      481 tccggcaccg gcacgcccgg gatgccgtca ccggcgcgca gcacggcggt ggcggcgtcg
      541 cggcactccg ggcaggtcag caggtgcagc agctcggcgc cgtcgtcgtg gcggagggcg
      601 gtggccagcc tccaagtcag gtgctccatc gtctcgtgcg ccatgcccgg ttccctcatc
      661 tagaggaaga ggaggtctcc ccgcgcccag agcgtccggt agatccaggc ctgcggcttg
      721 gtgctgaggt ccaggaggcg gctctggcgc agcagcccct tttcgggccg cccggcgcgg
      781 ctttgccgca ccgccttgag ccggaggctg gccagggcgt cccgggcgag gtcctcgtcg
      841 ccgggcagcc aggggggcgg cacggtggcg gcggcgagcg cctccgccgc ctctcccgac
      901 tccagcagcg cccgggcgag attgaagagg atggcctggc ggaccggcag tggcgcggac
      961 cggccggcct ccacggcgag gtggcgcagg tggtccgccg ccacaccggg ggctccctgc
     1021 gtggcgcgca ccatcgcctg gttgcagcgc acccacaggt ccgtctccac ctcaccggct
     1081 gcgtccccgg cccggtcgag cagctccccg gcgcggtccc attcgccgcg ggccgcggcg
     1141 acgaggccca ggttgttcag tggcaccgcg gtgccccagg ggccgtacgg ctccaggacc
     1201 tccaggcagg cgcgggcgtg gtactcggcg acgcccagca ggccgagctc gtagaaggcc
     1261 acggccaggt tgttgtggca cagcgcctct tccagctcga gcccggcctc gcccgcctgc
     1321 ttcagagcct gctccagatt gacgcgggcg aggccgggct ccaggaacag gcggctccgc
     1381 cgggcgagct ggagctggag cggcacgtcc ttcgccttcg tcgcgaggtc ctggagcttc
     1441 gagaagatgc ggctcgccgt tcgctcgtcc atctcgccac gctggaccag cgccagctcc
     1501 agttggatgc gggcggcctg cgccggatcg gcgctcgcgc cgaggagctg ccgggcctgt
     1561 tcgagccagc ggcgcgccgg gtagttggtg ggcggtgggg ccagccgcgc ggcggttccc
     1621 aggagataga gaaggccgga gccgccggcg cggaaggcgc cgtccgctcg cggcccgaga
     1681 tggtccacga tctcctcgta gcggccgagg ccgagcaggg ctcgcgcccg gatctccgcg
     1741 gcggccagcc tcgccgggct gtccggcgcc tccccggccc ggcccaggaa ccggtccatc
     1801 cacagcgcca ggcgtgcggc ctccgaaggc atctcttcat gcagggcctc cgacgcggcc
     1861 acggcggtct cttccgccgc ctgatgccac ttcccctcct cctccagcag agcggcgagc
     1921 cgcgggtccc cctcgtccag cgccggccgg cggtcctcgc agagcatccg gaccgcctcg
     1981 cggacccgct gatcggcgaa gcggaacgaa tcgggcctct cgtccgcgtg ctccagatag
     2041 ccggtccgct cgcccacgcg ctccagggcg ggtgccactg ccgggggcac ctccatccgg
     2101 gagcccagga cggcgccgcg ctcgatccag gagcgcgtct ccggcttctc gcgggccagc
     2161 gccgcctcca gtcgtcgccg gaccaactcc tcgaaacagg gcacgtccag agagctctcc
     2221 ggagcccgcc gccatcccat ggcgctcggc accagccgtt ccgtgtcgcg caggtagccg
     2281 aggatctcca gcaggtggcg ggggttgccg ccgctggcct cccgcagcca ggggaaggca
     2341 tgctcgaacc agggaggccc ccccatgcgc tcctggcagc acgccgccag ctcctccgga
     2401 ttcaaaggcg gcacttcgac gggccgctcg ctggataaga cgtccagggc cagccgcgaa
     2461 ggccccgtgc gggtcagcag gagcagcgag accgtgagcc gctcgcggcg ccgcagcatc
     2521 tcccggacgc actcccagga gggggcatcg aagctgtcca ggtcgtcgca gcagagcacc
     2581 aggagaggcc cgtcctggcc gcgcttccgg aacagggaga tccacgtctc gcgctcgcgc
     2641 acctcggccg ggactccggc cagctcccgc ggcagcgccg gctcctccag cggccacagg
     2701 cggagcgact cggcggcctc ccgcagaggc tggaacggcc tcccccaggc ggtgccccgc
     2761 gcccagacca tgcgggtggt gggcgccagc tccgcctgga gccgtcgcgc caggtgggtc
     2821 ttgccgatgc cggccgggcc gctgatcacc cggacgccga ctccggccgc gaagctcgcc
     2881 cgcaactcgc gcaggatcgc ctcgcgggac ggtttcggga aagggggagg cggcaggata
     2941 gggatcagga ggtcgtcgag aaggctcatc atggatagaa aaaagggggg gctacccctt
     3001 ggaggaaggg actgcgtcgg ggtagccccc tgaaaagccg ccgtgaccgg cccgttctac
     3061 agcgaggatc tgcctcacct gccgtagcag ctccggccac tctggcgctg gcgtcgaatc
     3121 gttcatggcc ggagccacct caggaagcgg ctcagccatg gcgtgcccgg ccggttcggc
     3181 cgcggttgga acctctccac gacggcggac ccggacatca aggccaccga gccgtcaccc
     3241 tcctgctcga ccttctggat ggcgtctcgg taggcgcggt gggccgcttc ccggtcctcg
     3301 ccgcggtgga tacagccggc gaaggaggag aggactctat agacaggcgg attcgcggcc
     3361 acgtggagac tcccattcca tccgtagatc ggggaccttg ccctgtcggc gccgtgcgtc
     3421 aaagctccgg cactcctgcg tcacccgggt caggaggacg tgatccggat cgggcagctc
     3481 ctcccataca cttcggggaa acgattcgac gacgagcgac agaatcggct ccgcctccca
     3541 gggggggacc acgaacgagg cgagcagccg gcgcatgcct tcccggttgc ggtgaaggat
     3601 ctctgccagg ctcggacttc gatttacagg ggcggtggtg tccatgtctc tcgctcgacg
     3661 gtcagccccc gccacttagg acgagcccgc ctcaagggtt cgcggcagcc ggcgggggct
     3721 gacgaggaga ggatgcccga ggttggagca gcgcggacgg cctcaccccc gctttacccc
     3781 tcgactgctg gtaggcacag gggatttctg taacatttgt tcaacgggcg atagagtttt
     3841 cgtagggccg atagctttct tgcggggtgt tgtccctgat ggcccccctt gcggcaacag
     3901 gtagagttga cagggaggat ctcgtggcga agacaccggc ggcaggcttg gtggtcacag
     3961 tgtgcaggga gatcgcgggc gtcacccagg ccgtcctggc gcggttcgcc ggcgttcacc
     4021 ggatggacat cgtgcgggcc gagaaggggc agggccgatt gacgccgggt ttcctggggc
     4081 ggctcatggg cttcccggag gcggtcaccg aggggttgcg cctgttggtg gaatggctcc
     4141 gtccggcccc cgaggagcgt tcgtttccgc ctctgggcct gccgctccgc gagcgccggc
     4201 tcatcgccgg cgccgccttg aaccatgccg ccgcccaagc cgagaggctc gccatggctc
     4261 ggctggcgga cctcatccac gccgatcggg agaacgcgaa gcaggattgg ctgctgatgc
     4321 gggcctggcc cgtcgaggaa tggcgaggct atctggagga ggcgccccag gtcgccacct
     4381 gggcgctggt cgagcggctc gccgaggagt ccacccgggc cgccgcagcg gacccccagc
     4441 gcgcgctcga gctggcggag ctggcttgct gggtggcgcc gcggattgag ggcacccggg
     4501 agtggcgcgg acgggttgaa ggctgggccc tggcgttcta cgccaacgct ctccgggtcc
     4561 tgggccgctt caaggaggca gaggcggcct tcgcccgggc ctggaccctc tggagggccg
     4621 gggcctcgct cggacccggc cctctcgacg agtcccgcct ctacgacctg gaggcctcct
     4681 tgcggcgcga cttgcgccag tggcccgaag cgttggccct gctcgacaac gcccttgccc
     4741 tatgcgagag ccctgaaggc gaggcgcgtg ttctcctcat tagagccttt actctggaac
     4801 agaaagggga tgctctggcg gcgctcgcgg tattggatcg ggcggaaccc ctggtggatg
     4861 atgagaagga accccgacag gtatttggtg tacggttcaa tcgcgctgtg aacctgctcc
     4921 acctcggccg gtccaacgag gctcaggcgc tgctcgcgga tttggaggag cgagtgcagc
     4981 gtctcgacaa cgacttcgat tctgcgcggt tgctttggct gcgctctcgc gtcgacgcac
     5041 gagttggtga cgaggaggca gcagaaggac gtctgctcca agtccgacag gagttcgcta
     5101 ggcccggact tgagttcgat gtagcactcg ctgatctgga actcgcccgg ctttacctga
     5161 ggcaggggag aaacgcggaa acgcgggagt tggccgctgc tgcatggccc gtctttcggc
     5221 gcctgggtgt ggcacgcgaa gcggcggagg caatccagct cttttggaaa gcagcacgcc
     5281 gggagcgagc tacggttgat atggccgatc gggctcttga ggcacttctg cgcgcttggc
     5341 cctgcccagc ggcttgaaga gcggccgagc cgcgaggcgc ggctcggccg caactagcag
     5401 cttatgaaag gcggaattcc aataatgcta cttcgcacac agcccgttcg ggtcgatgcc
     5461 acagccccct tccccctgga ctggaggggt ggtctgggtg gtcaagcaac tcccgtttgg
     5521 atcgatgata cagccccctt ccccctgggt ggtctgggtg tttgagtcgc gcccatctgg
     5581 gtcaatgagg caaccacctt cgctgccact gggatcgatg ccgcagcctc ctttctccca
     5641 gaagcgggtg acgagtgtcc acgctcgagc gaggagatcg gcaggaaccg agaggcttct
     5701 ggctgtcgtg gagtgcagcc cagaactccg cggctgggca gtggcagccc agggagcgag
     5761 cagaacgagt ccgaaggcga cgatcaggac gaggcggtga atgggacgag acatgcggcg
     5821 ctcctcttcc gggccgttcc cggagcggcc tccatggtca ctcggtggcc cttggagggt
     5881 atggtgccgc aacgcggtga acaaatgttc tggaaatccc ctgtaagcac ctgggatatc
     5941 tctgcgagga gaggcagatg gcccaccacg aggtgaaggc aagaaccgcc atgatcgtgc
     6001 ggctcatccg gtttctgcgg gaatggagtc aggaagagct ggcgaagcag gccggagtcc
     6061 attcctcgga ggtctaccgc tgggagacgc aggtctacgc gccgtctcgt gccaacctgg
     6121 aacggctcgc ggcggcggca cgagtccccc tcggcctgct agagattctg gcggtcttcg
     6181 tgctctggct cctgtggctc ggcggggcgc gctcggccgc cggccaagcg gaggaggaac
     6241 cgcaggagag gctggtccgc gccgcccagg agggagtcgc gttggagatg gcccagccac
     6301 tttgcgggct gcctcgcgcg gagagatggg agcgcccgcc ggaggacgcc gcaacgcgga
     6361 gcgagatcac tgtcgtttgg ctctgcgccg agagcgtgcg ggcggcggga tccgatgcgg
     6421 cccgcgctct gtcgctcaca cgagcggcat tacgggtagc acggaaggcc gagccagagc
     6481 atcgcctctt cctggaagcc tacgttctac cgttcgaagg aaatgccttg cgagtgggaa
     6541 gcgatttcca gcgggccgaa acggccttcg ttcgcgcaag ggcactccgc ccggctctct
     6601 ttgcgccggg tagagagaga ctccacctct cccggctcct cgatctggag gcgtctctcc
     6661 ggcgtgacca aggccggttt cccgaggctt tggatttgct tgatcgggct ctcgcggcaa
     6721 gccgcacacc ggcggccgaa gggcatgtcc tcctcaatcg agccttcacc ctggaacaga
     6781 tgggggacat tccgggcgcc gtgaccgcgt tgcagcaggc agcccagaag atcgatggcg
     6841 agagagagcc gcggcagttg tttggggccc agttcaatct tgtggtgaac ctgtgccacc
     6901 tgggtcggtt ccaagaggcg gagaagctgc tgccttccgt ctggatgctg gcggcggagc
     6961 tggaaaaccg gaacgacctc gtgcgggtct cctggctgga gtcccgcgtc ctcgccggca
     7021 agggagacct ggagaaggcc atctccaccc tggagcgggt gcgggaagcg ttcgcggagc
     7081 ggaagaacct gtacgacgta ggccgttcca ccctggagct ggcccgcctc tatctccgcc
     7141 agggccgcac cgacaagacg aaggaactag ccgcccgggc cgagcccgtc ttcagggagc
     7201 tgaacgtcgc ccctcgggcc ctggagtcgg tcctcgtctt cctggaagcc gcccgccagg
     7261 aagccgccac cctggagctg ctcgaccagg ccctccaggc cctggggcag gggcggcggg
     7321 ggcggtaggg ggaaggtata tactgacctg cctaaacagg agggaacctg atgaggccga
     7381 gcatcaagcg gctgctgttt gccggagcca tcaccctgac gctgtttgtc ccgcgtccag
     7441 cccaggctta cgtcatttgc aatcattacg actgcatgag tgtttgcacc ttttacgacg
     7501 atcaggataa cgttttacgg aagtccttca tccccacgca gtgctcgatg tgaggttcga
     7561 tgatgaaaag gcgtgacgct tggttctttt cggctctgtt gttcggcgcc agctttctgg
     7621 aggcggccga gatggaccgg gcgctcacgc aggtccgccc ccagcatctg gcgccctcca
     7681 gcacggcctg gagcggtgcg cagtttgtcc aagcagatcg cgaggggcgg atcttcttgc
     7741 tgaggtccga ggctctcgag gtctaccccc tggaagcgga cggccagttc ggtgaggtcc
     7801 agaggctgaa ggcccttccg agtagtggtc cgttccttga acgtgtgttc ggagcggtga
     7861 tgatggggcc gggagactgg tttctggagc agggtggcag ggtccgcctg tttcgcggag
     7921 ggcaggagga gtccctggag gatccgcgtt ggcagatctc cgggcttggg gccagcgggg
     7981 gagaccctgt gatcgcggga acctcgggtg aagccatggc ccaggtcttt ctcagaaaag
     8041 agagcgcgaa accggctccg ctgatgctga cctg
//
LOCUS       DASPJI010000279         3639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_417237, whole genome shotgun sequence.
ACCESSION   DASPJI010000279 DASPJI010000000
VERSION     DASPJI010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417237"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VEL74_07380"
     CDS             <1..430
                     /locus_tag="VEL74_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG62386.1"
                     /translation="ELFLRLKDSYAELFSHAGVLFRTTNLLLFEHVHLPMEYRQNLYL
                     IFKEALNNSLRHSGCREIELSVALRGRHLEVVLKDDGRGFDPGELEGRGDGLENMRRR
                     ASRIGGSLDIDSAPERGTAVAFSGPLASGAGGLSGAGGGA"
     gene            427..1110
                     /locus_tag="VEL74_07385"
     CDS             427..1110
                     /locus_tag="VEL74_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYG62387.1"
                     /translation="MIRVAIVEDNQTIQQGLSMLIDGTPGYACVGAYTDCESLLAEIA
                     EVRPGVLLMDIGLPGMSGIEGVRRVKAVMPEVNILILTVYDENDLVFEALCAGACGYL
                     VKKTPPARLLEAIQEAHGGGSPMSSHIARKVVNLLRQTSGKKAPALPIGGLAAARRET
                     PGDEAMLTARERDILNGLAEGNSYQALAKNLNISADTVRFHIRNIYRKLHVHSQSEAV
                     AKALRKGII"
     gene            1116..2630
                     /locus_tag="VEL74_07390"
     CDS             1116..2630
                     /locus_tag="VEL74_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYG62388.1"
                     /translation="MNLSDLFAIPPRQTPAKPALRFRDGDTAADLTYEELFAAAGRLA
                     AHFHSLGLKKGDRAAFFLGNRPEFVIAYLALIRLGAVLVPINLAYRRREIAHILEDAA
                     PRLLVTERSQLPFLEELDPAERQTLDSILLVEDLWAAVPPRLGQEGGLSGAGPIHGSD
                     LAMILYTSGTTGRSKGAMISHDNVLATMTGLLAAWAWRPDDALLLSLPLFHTHGLVVG
                     LHCALAAGATVLLRRRFDAAETVRDLLSGEPTLFFGVPTMYVRLADELKTVADRSALA
                     RMRLFCSGSAPLAPETFAAFRDLTGHAILERYGMTETGMNLSNSYAGERVPGTVGTPL
                     PGVSARIVDAAGRDVEPGGEGELLVRGSNVFSGYWQAPEKTDESFTHDAAGWRWFKTG
                     DLARQDPATGAITLLGRRHELIISGGFNVYPREVEEVLVTFPGVREAAVVGRRHPEWG
                     EVPAAFLVTDGEIDETELIIFCKSQMAGFKVPREVRFVEALPRNALGKVQKHLL"
     gene            2717..>3639
                     /gene="nhaA"
                     /locus_tag="VEL74_07395"
     CDS             2717..>3639
                     /gene="nhaA"
                     /locus_tag="VEL74_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HYG62389.1"
                     /translation="MPKRPTLALLRPFQTFFRLESASGILLLASAVLALIWANSPLSP
                     YYFGLWETNVTASVGGFAISKPLLLWINDGLMAIFFFVVGLEIKREVLVGELASPKKA
                     ALSIAAAIGGMLVPAGIYAFLNSGGAGSSGWGIPMATDIAFTLGVLALLGRRVPLALK
                     VFVTAVAIVDDLGAVLVIALFYTAEVDGVALLGAAAVLGTMALMNRAGVRHPLAYLAV
                     GVVLWFAVHASGVHATIAGVLAAMAIPSRTRIDSQEFLVGGRRILDEFDDAGVEGESV
                     LSNHGQQAAIQALETRCEAAQAPLQRIEHDL"
BASE COUNT          515 a         1339 c         1222 g          563 t
ORIGIN      
        1 cgagctgttc ctgcggctca aggactccta tgccgagctg ttctcccacg ccggggtgct
       61 gttccgcacc accaacctcc tcctgttcga gcacgtgcac ctgcccatgg agtaccggca
      121 gaacctctac ctcatcttca aggaggcgct caacaactcg ctgcgccaca gcggctgccg
      181 cgagatcgag ctgtccgtcg ccctgcgcgg ccgccacctg gaggtggtcc tcaaggacga
      241 cggccggggg ttcgatcccg gcgagctcga gggccggggg gacggcctgg agaacatgcg
      301 gcgccgggcg tcgcgaatcg gcggcagcct ggacatcgac tcggcgccgg agcgggggac
      361 cgccgtcgct ttcagcgggc cactcgccag cggggccggc gggctcagcg gcgccggagg
      421 gggagcatga tccgcgtcgc catcgtcgag gacaaccaga cgattcagca ggggctctcg
      481 atgctgatcg acggcacgcc cgggtacgcc tgtgtcggcg cctacacgga ttgcgagtcg
      541 ctgctcgccg agatcgccga ggtgcgcccg ggcgtgctgc tcatggacat cggcctgccc
      601 ggcatgtcgg gcatcgaagg ggtgcgccgg gtcaaggccg tgatgcccga ggtcaacatc
      661 ctgatcctca cggtctacga cgagaacgac ctggtgttcg aggcgctctg cgccggggcc
      721 tgcggctacc tggtgaagaa gacgccgccg gcgcggctgc tcgaggcgat ccaggaggcc
      781 cacggcggcg gctcgccgat gagctcgcac atcgcccgca aggtggtgaa cctgctgcgc
      841 cagacatccg gcaagaaggc gcccgcgctg cctatcggag gtctggcggc cgcgcgccgc
      901 gagacgcccg gcgacgaggc gatgctcacc gcccgcgagc gcgacatcct gaacggcctg
      961 gccgagggca acagctacca ggcgctggcg aagaacctca acatcagcgc cgacacggtg
     1021 cggttccaca tccgcaacat ctaccgcaag ctccacgtcc actcgcagtc cgaagcggtg
     1081 gccaaggcgc tgcgcaaggg gatcatctag gagccatgaa cctctccgac ctcttcgcga
     1141 tccccccgcg gcagactccg gccaagccgg cgctccgctt ccgcgacgga gacacggcgg
     1201 ccgacctgac ctacgaggag ctgttcgccg ccgccggccg tctcgccgcc cacttccact
     1261 cgctggggct gaagaagggg gaccgcgccg ccttcttcct tggcaaccgg ccggaattcg
     1321 tcatcgccta cctcgcgctg atccgcctcg gcgccgtcct ggtcccgatc aacctggcct
     1381 atcgccgccg cgagatcgcc cacatcctgg aagacgccgc gccgcgcctc ctcgtcaccg
     1441 agcgctcgca actccccttc ctggaggagc tggaccccgc cgagcgccag accctcgact
     1501 cgatcctcct cgtggaggac ttgtgggcgg cggtgcctcc ccgtttgggt caggaggggg
     1561 gtttgtctgg agccggcccc atccacggct ccgacctcgc catgatcctc tacacctccg
     1621 gcaccaccgg ccgcagcaag ggggcgatga tcagccacga caacgtcctg gccacgatga
     1681 ccggcctcct cgccgcctgg gcctggcggc cggacgacgc cctcctcctg tcgctgccgc
     1741 tcttccacac ccacggcctc gtcgtcgggc tccactgcgc cctggccgcc ggggccacgg
     1801 tcctgctgcg ccgccgcttc gacgcggccg agaccgtccg cgacctcctc tccggagaac
     1861 cgacgctgtt cttcggcgtc cccaccatgt acgtccgcct ggccgacgag ctgaagaccg
     1921 tggcggaccg ctcagccctg gcccgcatgc gcctcttctg ttccggcagc gcgccgctcg
     1981 cccccgagac cttcgccgcc ttccgggacc tcaccggcca cgccatcctc gagcgctacg
     2041 gcatgaccga gaccgggatg aacctgagca attcctacgc gggggagcgg gtgcccggca
     2101 ccgtcggcac gccgctgccc ggggtctccg cccgcatcgt cgacgccgcc ggccgggacg
     2161 tggagccggg cggggagggg gagctgctgg tgcgggggag caacgtcttc tccggctact
     2221 ggcaggcgcc ggagaagacc gacgagagct tcacccacga cgcggcgggc tggcgctggt
     2281 tcaagaccgg cgacctcgcc cgccaggacc ccgccaccgg cgccatcacc ctcctcggcc
     2341 gccgccacga gctgatcatc tcgggcggct tcaacgtcta cccgcgcgag gtggaggagg
     2401 tgctcgtcac cttcccgggc gtccgcgagg ccgcggtggt gggccgccgg catccggagt
     2461 ggggcgaggt ccccgcggcc ttcctggtga cggacggcga gatcgacgag acggaactga
     2521 tcatcttctg caagtcgcag atggccggct tcaaggtgcc gcgggaggtc cgcttcgtgg
     2581 aggccctgcc gcgcaacgcc ttggggaagg tgcagaagca cctgctctga ggagccctgt
     2641 ctcctccggg aaggtaagtc tctgctctgc aattttcgcc cgtcttcgat aaagtccggg
     2701 gttggaggga gagagagtgc cgaaacgacc caccctggcc ctgctgcggc cattccagac
     2761 ctttttccgg ctcgaatccg cgagcggcat cctgctgctc gcctcggcgg tgctcgcgct
     2821 gatctgggcg aattcgccgc tgtcccccta ctacttcggg ctgtgggaga ccaatgtcac
     2881 cgccagcgtc ggcggcttcg ccatctcgaa gccgctgctc ctctggatca acgacggcct
     2941 catggccatc ttcttcttcg tcgtcggcct ggagatcaag cgcgaggtgc tggtggggga
     3001 gctggcgtcg cccaagaagg cggcgctgtc catcgccgcc gcgatcgggg ggatgctcgt
     3061 cccggcgggg atttatgcct tcctcaactc cggcggcgcg gggagctccg gctggggcat
     3121 tcccatggcg acggacatcg ccttcaccct cggcgtgctg gcgctcctcg gccggcgcgt
     3181 gccgctggcg ctcaaggtct tcgtgaccgc ggtggcgatc gtcgacgacc tcggcgccgt
     3241 gctggtgatc gcgctcttct acaccgccga ggtcgacggg gtggcgctgc tgggcgccgc
     3301 cgcggtgctc ggcaccatgg cgctgatgaa ccgcgccggc gtccgccacc cgctcgccta
     3361 cctggccgtc ggcgtggtgc tgtggttcgc ggtgcacgcc tcgggggtcc acgccaccat
     3421 cgccggcgtg ctggccgcca tggcgatccc ctcgcgcacc cgcatcgact cccaggagtt
     3481 cctcgtcggc ggccgccgca tcctggacga gttcgacgac gccggcgtcg agggcgagag
     3541 cgtgctctcc aaccacggcc agcaggcggc gatccaggcg ctggagacgc gctgcgaggc
     3601 ggcgcaggcc ccgctgcagc gcatcgagca cgacctgca
//
LOCUS       DASPJI010000280        14100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_530705, whole genome shotgun sequence.
ACCESSION   DASPJI010000280 DASPJI010000000
VERSION     DASPJI010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14100)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14100
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530705"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VEL74_07400"
     CDS             complement(<1..333)
                     /locus_tag="VEL74_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62390.1"
                     /translation="MEMALFIHILGGGLGLVSGYIALFAAKGARLHRQSGMLFVYAML
                     GMSLIGAVVAALSATEGSVIAGLLTAYMVITALTTVRPAVAGLRWLNITLMALALAVG
                     VTSLAWGVQ"
     gene            complement(359..1264)
                     /locus_tag="VEL74_07405"
     CDS             complement(359..1264)
                     /locus_tag="VEL74_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG62391.1"
                     /translation="MSSRTDTSLVNDPQPAAPASLPETRPIYWSVRRELWENRSIYIA
                     PLIVAGVVFLGLAIHTLSVPGPKSTLPGLQAYAAGSYNMAPAPIMLATFLVGLFYSLD
                     ALYGERRDRSILFWKSLPVSDLTTVLSKAIVPLVALPLIAFVLSVATKFFLALFGILV
                     LLANGKSPSAFWTQLHFPQEMVVMAYGLAVHTLWFAPIYGWALLLSAWARRVPFLWAV
                     LPPAMIFVAGNHLFNSPYLASMLKYRVTGAMETAFTSIDGKIGDLSKLTPSRFLSTPG
                     LWIGLIFAAVCIAAAVRLRRKREPI"
     gene            complement(1261..2124)
                     /locus_tag="VEL74_07410"
     CDS             complement(1261..2124)
                     /locus_tag="VEL74_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG62392.1"
                     /translation="MSSIEARGLRRAFGSTIALDGIDLRVEEGRVLGLIGPNGAGKST
                     ALHAILGLIPYEGELRVLGLDPWTERDRLMRDVAFIADVAVLPRWIRVSQVLDYVAGV
                     HPRFDRAKAEEFLARTTIKRTSKVRELSKGMVTQLHLALVMAIDARLLVLDEPTLGLD
                     LLVRKQFYDSLLNDYFDRGRTILVTTHQVEEIQDVLTDFMFIDRGRVVLASSMEEFES
                     RYLEVMVPPDCLAAARALGPLRERQILGRSILLFNHAGRQQLAALGDVRTPSIADLFV
                     AVMDHPQGAAQ"
     gene            complement(2129..2506)
                     /locus_tag="VEL74_07415"
     CDS             complement(2129..2506)
                     /locus_tag="VEL74_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HYG62393.1"
                     /translation="MDREWNDSQPIYRQIHDLVVEMILDRLLGEGDPVPSVRNVAAEY
                     RVNPLTVLKSYQQLVDEGLLEKKRGRGLFVKAGARDLLLRDERRRFLTEQWPKVVETI
                     RRLGLSPRELLDNAAIQESEKER"
     gene            2615..4039
                     /gene="rimO"
                     /locus_tag="VEL74_07420"
     CDS             2615..4039
                     /gene="rimO"
                     /locus_tag="VEL74_07420"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HYG62394.1"
                     /translation="MSEPSNRPRVGMISLGCPKNLVDSEIMLGELARRGYEVVADLDS
                     ADTVVVNTCAFIEEAQQESIDTILEVVGRKGQGVEKVLVAGCMVNRFGQELSRSIPEI
                     DGFVSLDQLREVGDLVQLGGGPPLPAPSHMVFDHTAPRLLTTQGHAYLKVAEGCNNPC
                     TFCAIPVWRGRFRSRTIESLLAEAKQLEAAGITELNLIAQDTTRYGEDLGLRRHGLLR
                     LVEALLAETSIPWIRFLYAYPTTLDEDLLRLMGTEERFCSYLDMPLQHSHGDILSAMR
                     RGGDARRYLRLLERARELVPDISLRSTFIVGFPGETEEHFRDLMAFIERARFDHLGAF
                     AYSPEEGTPGAGLSGRVAKNVARRRLRQLLEAQKPIALESRRRLVGRRLEVLVEGVCE
                     ESEHLLQGRHRGMAPQIDGRLLINDGDAPAGTLVEVEITEAYADDIVGHITGAARPQD
                     RDRVRIAESAEISSLEGEPAAFFA"
     gene            4057..5034
                     /locus_tag="VEL74_07425"
     CDS             4057..5034
                     /locus_tag="VEL74_07425"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HYG62395.1"
                     /translation="MLVIQDAYNSSDLPHGGVGAIGNFDGVHRGQRAILDLVVARARE
                     LQVPAVVITFDPHPLTVLRPGQAPTPLTTLRQKEALLDEAGVSVVLVVRFDQDLARTP
                     ARVFVRDFLHGRLGLQEIYVGSGFVFGHQREGDLPLLRQMGESLGFKAFGVEEVLQEG
                     ERISSTRVRRAISEGKVTEAAEMLGRPYTISGIIARGDRMGQRLGWPTINLVPENKLL
                     PADGVYAGRVFFPSFPATFDCVTNIGTRPTVYENYQRVVESHILDFKSDVYGARVDIS
                     FYKRLREERIFPTVMDLAAQIGRDVEATREYFSRRRMEQEVPSAGLERE"
     gene            5074..5463
                     /gene="trxA"
                     /locus_tag="VEL74_07430"
     CDS             5074..5463
                     /gene="trxA"
                     /locus_tag="VEL74_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HYG62396.1"
                     /translation="MRCRGEHALRAAPTRNGEREMASEKIVTISEDNFENEVLKSDQP
                     VLVDFWAEWCGPCRMVAPVLDQIADEMDGKVRIAKLNVDENQKIAYQFQVSSIPTFIL
                     FKNGQVADRTMGAMPKNAFQSFIDRNI"
     gene            5524..6780
                     /locus_tag="VEL74_07435"
     CDS             5524..6780
                     /locus_tag="VEL74_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HYG62397.1"
                     /translation="MSSPDALDGRLAFLNAGLDELGCQALLVFAPSAADPDLAVFTGS
                     VHLGESLLVAPRGERPRLAYLTPMERDEAAATGLLLITPEELDVLRWTQEAPEPVDFT
                     AAVTARALELAGLSPGRVALAGHGRVGVLHAVLSRLARSGWSWVPANDLLTGLRKGKV
                     AGELAAIRAAAAGTVEAFRTVARLLAAAGLRAGEGDELWLEGERLTVARLRAEVARTL
                     AGFGLEQPRGNIIAPGEEGGVPHSTGTQERVLRAGESLVVDLFPRGTLFADCTRTFCV
                     GTPPEPLARAHAAVLAALRAAHAGATAGATSTIRGWDLQEAVCHSLGDAGYPTPISHP
                     GTTRGYVHNLGHGVGFELHEQPIFKKVAGREGVLAHGDVFTLEPGLYDPEAGYGVRLE
                     DLVHLTPGGLENLTPLPYDLDPRAWG"
     gene            6847..9285
                     /locus_tag="VEL74_07440"
     CDS             6847..9285
                     /locus_tag="VEL74_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG62398.1"
                     /translation="MSDETAAYEKLGAFYLGRPYDLERGEARPVPLLYDSRDLLTHAV
                     CVGMTGSGKTGLLIGLLEEAAIDGIPSLVIDPKGDLANLLLTFPRLAPGDFVPWVDAD
                     AAGRAGISPEDFAAREAETWKNGLGQWGQSGERIARLREAADFAVYTPGSESGLPVSI
                     LASLAAPPADVLADGDLLRERVSGLAASLLTLLGIEGDDADPVRGRDHILLSTLFDAA
                     WREGRGLDLATLIQQIQKPPVERVGVLDLESFYPAKERFALAMAVNNLLGSPGFAAWL
                     RGEPLDVDRLLYTPSGKPRVAVFSIAHLGDRERMFFVSLLLQQTLAWMRSKPGTSSLR
                     AILCMDEVFGYMPPVAEPPSKRAILTLLKQARAFGLGLVLATQNPVDLDYKGLGNVGT
                     WFLGRLQTERDKQRLLDGLQGAAGGMDRGELERMLSQLGKRVFLLHNVHEEEPEVFQT
                     RWTLSYLRGPLTRTEIKRLMDPRKGGTPAATATAPAPSAPSAASDSGTGAPPVLPPDI
                     SQRFLPVCSRPSGIVYRPALFAAAKVHFADAKQGIEHEEETALLALMAAGEGEPVDWY
                     AAEGVELWKEDLEPEPVAGARFEPLPDAAVRAKSYDGWRRDLEECLYRTRRCDLFRSP
                     ALGVTSRPGELERDFRIRLSDLAREKRDGQVETLRKKYASKVAQLEDRIRRAEQTKER
                     QEDQVRQQGWQTVLSAGTAVAAMLFGRKKLSHANLGRATSAVRGVGRTFQERQDVSRA
                     QENVEALQRQLADLNTELEAEIGKLEARFDPEAEPLETVSLKPRRTDVEVRFLSLAWA
                     PGRPGDAGWEPAWR"
     gene            9456..10019
                     /locus_tag="VEL74_07445"
     CDS             9456..10019
                     /locus_tag="VEL74_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HYG62399.1"
                     /translation="MEVFLSILVGLGLSAACGFRIFIPPLILSLAARGGHLELGDSFA
                     WMGSNAALISFAVATVLEIAGYYIPWVDNLLDAIATPTAVVAGILVSASTFTDVTPFL
                     KWTLAILAGGGSAAVFQGITATARQISSLSTGGLGNPLLATVEAAGSALLAVLSVTLP
                     LLAILVVVGLLYFGVRRLLFRRPVRVT"
     gene            10060..11232
                     /locus_tag="VEL74_07450"
     CDS             10060..11232
                     /locus_tag="VEL74_07450"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG62400.1"
                     /translation="MKLMLDFKRRSRVRHTSLGATLLAVAGAMALGGCATGGPPDQGD
                     RSQIAPTLQARGVDPNAVVIPYELTDEMREWVHRMVPRNLSFDRRLPMLVDALLSEKG
                     LNVTYEAKHTGTAEEVFKTRRANCLAFTSLFVAMSREVGVPAFFLDVQELERFEKEGD
                     LVVVSGHVSAGYGIGKTLQVLDFSSVSPTSGYQRRVFPISDVRAIALYHSNRGAEALR
                     AGNEEEALDWLHKAVAIDPEVPGAWINYGVALRRSGKDGEAENAYRKALEIDSGAVSA
                     YQNLASLLRYKGRTQEAEQLMALTLRMGTRNPYNYLALGDLSLSHGRFDEARRYYKRA
                     MRLYRDNPEPYAALGIISLIDGDRLEATKWLRKASAIDQENNRVKQLQARLGSQVG"
     gene            11243..11665
                     /locus_tag="VEL74_07455"
     CDS             11243..11665
                     /locus_tag="VEL74_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HYG62401.1"
                     /translation="MRSLTRHLTMNVPARMDFVNITPDVERAVAESGVQEGLCLVNAM
                     HITASVFINDDEPGLHRDYKRWLEELAPFDASSQRYHHNRTGEDNADAHHKRQIMGRE
                     VVVAITGGRLDFGPWEQIFYGEFDGRRPKRVLVKVIGE"
     gene            11677..13881
                     /locus_tag="VEL74_07460"
     CDS             11677..13881
                     /locus_tag="VEL74_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HYG62402.1"
                     /translation="MAGGWRIWVDTGGTFTDCLAVDPSGGLHRAKVLSTSALRGRVEE
                     RAGPTSLRLAAPWRVPADFFAGFTFRLLGGGASQAAAVTGYDPAGVLDLAAPIDGSLS
                     GATFELRSPEEAPVLAARLVTGTPLGWALPEIALRLATTRGTNALLERKGAATALFVT
                     RGFADLLRIGTQQRPDLFALEVRRPEPLYREAVEVPERLAADGSVVEPLDLETLRATA
                     ERLLADGVVTAAVALMHGYRNPAHEEELGAFLAGLGFRHVSLSSRLAPLIKLLPRAET
                     AVVDAYLSPLLADYLAGVSRAISHGSLHVMTSAGGLVRAEAFRAKDALLSGPAGGVVG
                     AALSGRRSGFRRVIAFDMGGTSTDVARWDGDFEYLFEHRVGDAHLVAPALAIESVAAG
                     GGSICSFDGAQLRVGPESAGAAPGPACYGAGGPLTLTDVNLLLGRLDPGRFEIPLDPG
                     AAERALGELLEGMAARGPAPEPEAVLAGLLDIANEKMAEAIREVSVRRGYDPADHALV
                     AFGGAGAQHACAVAELLGIGSVLVPVDAGLLSALGLGAAVVERFAQRQVLAPLVQVEG
                     HLQGWLDELGRQAVSDVVAEGMLPESVRVRRRLLNLRFTGQETALTVEYGEEPVDQAF
                     AAAYREIYGYAPEGRAVELESVRVVASSSAEEEVLPAEAATRFPAAPEGSRRCCFGGS
                     WRDVPFFDRAGLRPGASFQGPALVFEAHSATVVAEGWDGMADGAGGLVLAQR"
     gene            13903..>14100
                     /locus_tag="VEL74_07465"
     CDS             13903..>14100
                     /locus_tag="VEL74_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62403.1"
                     /translation="MQENWNLIQREAFFAQRLQPLRRICAALAANAVLMAAVAWFLLG
                     PGGFVPPRLPDAVPLSITAASA"
BASE COUNT         2095 a         4828 c         4984 g         2193 t
ORIGIN      
        1 ctgaacgccc caggcgaggc tggtgactcc gacggccagc gccagcgcca tgagagtgat
       61 gttcagccag cgcagccccg cgacggcggg acggacggtg gtcagtgcgg tgatcaccat
      121 ataggccgtc aataatccgg cgatcaccga gccctcggtg gcgctcagtg ccgcgaccac
      181 cgccccgatg agcgacatcc cgagcattgc gtagacgaac agcatcccgc tctgccggtg
      241 caggcgcgcc cctttggccg cgaagagcgc gatgtaaccc gacacgagtc ccagcccacc
      301 tccaaggatg tggatgaaaa gcgccatttc catggcgatc ctcctgcttg tttttagttc
      361 agatcggttc acgtttgcgg cgcaggcgca ccgctgccgc gatgcacacc gcggcgaaga
      421 tcagcccgat ccagagaccg ggcgtgctca ggaatctcga cggagtgagc ttcgacagat
      481 ccccgatttt cccatcgata gatgtgaagg ctgtctccat ggcgcccgtc acacggtact
      541 tcagcatgga ggcgaggtac gggctattga acaagtggtt ccctgcaacg aagatcatgg
      601 ccggaggcag gacagcccac aggaagggca cacgccgggc ccaggcggag agtagtagcg
      661 cccagccata gatgggtgcg aaccagaggg tatgcacggc caggccgtag gccatcacta
      721 ccatctcctg gggaaagtgg agctgggtcc agaaggcaga gggactcttg ccattggcca
      781 gcaggaccag gatgccgaag agtgccagga agaatttcgt cgccacgctg agcacgaacg
      841 cgatcaacgg cagcgccacc agtggaacga tcgctttcga gagaacggtg gtcagatccg
      901 aaaccggcag cgacttccag aaaaggatgc tgcggtcgcg gcgctcgccg tagagcgcgt
      961 cgagtgagta gaagagcccg acgaggaagg tggccagcat gatcggagcc ggcgccatgt
     1021 tgtacgaccc cgcggcgtag gcctggagac cgggaagggt gctcttgggc ccaggcacgc
     1081 tgagggtgtg gatggcgagg ccgaggaaca cgactccggc aacgatcagc ggcgcgatgt
     1141 agatcgagcg gttctcccac agctcgcgcc ggaccgacca atagatcgga cgcgtctccg
     1201 gcaaggacgc gggcgcggcc ggctgagggt cgttgacaag tgaggtgtcc gttcgagagc
     1261 tcattgagcc gctccttgtg gatgatccat gacggcgacg aacagatcgg cgatgctggg
     1321 ggtgcgcacg tcacccagcg cggcgagctg ctgacggccg gcgtggttga agagcaggat
     1381 gctccggccg aggatctggc gctcccggag aggcccgagc gcccgtgccg cggccaggca
     1441 atcgggaggc accatcactt ccagatagcg cgactcgaac tcttccatgc tgctggcgag
     1501 cacgacgcgg ccgcggtcga tgaacatgaa gtcggtgagg acgtcctgga tctcctcgac
     1561 ctgatgcgtg gtcacgagga tagtgcggcc ccgatcgaaa taatcgttca gcagggagtc
     1621 gtagaactgc ttgcggacca ggagatcgag gcccagcgtc ggctcgtcca gcaccagcag
     1681 tctcgcgtcg atggccatga ccagcgcgag gtgcagttgg gtcaccattc ccttcgacag
     1741 ctctcggacc ttgctcgtgc gcttgatggt ggtcctggcg aggaactctt cggccttggc
     1801 gcggtcgaac cgcggatgga cgccggcgac gtagtcgagc acctgggaga cccggatcca
     1861 gcgcggcagc acggcgacgt cggcgatgaa ggcgacgtcg cgcatgagcc ggtcgcgctc
     1921 ggtccaggga tcgagcccga ggacccgcaa ctccccctcg taggggatca ggccgaggat
     1981 cgcgtgcagc gcggtgctct tgccagcgcc gttgggaccg atcaacccga ggacgcgccc
     2041 ctcttccacg cgcagatcga tgccgtccag cgcgatggtg gatccaaagg cccttcgcag
     2101 tccgcgtgct tcgatggatg acatgctctc aacgctcctt ttcggattcc tggatggcgg
     2161 cgttgtccaa tagctcccgc ggcgagaggc cgagcctccg gatggtctcg acgaccttcg
     2221 gccactgttc cgtgaggaac ctccggcgct cgtcccgcag cagcaggtcg cgcgcgccgg
     2281 ccttgacgaa caggccgcgc ccccgcttct tctccaggag cccttcgtcg accagttgtt
     2341 gatagctctt gaggacggtg agcgggttga cccggtactc ggcggccacg ttgcgcaccg
     2401 acggcaccgg atctccctcc ccgagcagcc ggtcgaggat catctcgacc accaagtcgt
     2461 ggatctgccg gtagatcggc tggctgtcgt tccattcgcg gtccatgttc gagctcaggt
     2521 gcggtgatgt agtcaactat aacaccatat cgagggaatg caagtgggtg ccggaaatga
     2581 tccggcgttc tcatctgtta gactctcccg gcccatgagt gaaccctcga accgcccccg
     2641 cgtgggcatg atcagcctcg gctgcccgaa gaacctcgtc gacagcgaga tcatgctcgg
     2701 cgagctggca cggcgtggct acgaggtggt cgccgacctg gactccgccg acacggtggt
     2761 ggtcaacacc tgcgccttca tcgaggaggc ccagcaggag tcgatcgaca ccatcctgga
     2821 ggtggtgggc cgcaaggggc agggggtgga gaaggtgctg gtggccggct gcatggtcaa
     2881 ccgcttcggc caggagctct cccggtcgat ccccgagatc gacggcttcg tcagcctcga
     2941 ccagctccgc gaggtgggcg acctggtgca gctcggcggc gggccgccgc tgcccgcgcc
     3001 gtcgcacatg gtcttcgacc acacggcgcc gcggctgctg accacccagg ggcacgcgta
     3061 tctgaaggtg gcggaggggt gcaacaaccc ctgtaccttc tgcgccatcc cggtctggcg
     3121 ggggcgcttc cgcagccgca ccatcgagag cctgttggcg gaggcgaagc agctcgaggc
     3181 ggccggcatt accgagctga acctcatcgc ccaggacacc acccgctacg gcgaggacct
     3241 ggggctgcgc cggcacggcc tgctgcgcct ggtcgaggcg ctgctcgcgg agacgtcgat
     3301 cccctggatc cgtttcctct atgcctatcc gacgacgctc gacgaggacc tgctgcggct
     3361 gatggggacg gaggagcgct tctgctccta tctcgacatg ccgctccagc acagccacgg
     3421 cgacatcctc tcggccatgc ggcggggcgg ggacgcgcgg cgctacctgc ggctgctgga
     3481 gcgggctcgc gagttggtcc ccgacatctc cctgcgctcc accttcatcg tcggcttccc
     3541 cggcgagacc gaggagcact tccgggacct gatggcgttc atcgagcgcg cccgcttcga
     3601 ccacctgggg gcgttcgcct acagccccga agaggggacc cccggcgccg ggctgtccgg
     3661 ccgggtggcg aagaacgtcg cgcggcggcg gctgcgccag ctcctcgagg cacagaagcc
     3721 gatcgcgctg gagagccggc ggcggctggt cgggcggcgg ctggaggtgc tggtcgaggg
     3781 ggtctgcgag gagagcgagc acctgctcca gggccggcac cgcggcatgg cgccgcagat
     3841 cgacggccgg ctgctgatca acgacggcga cgccccggcc ggcaccctgg tcgaggtgga
     3901 gatcaccgaa gcctacgccg acgacatcgt cggccacatc accggcgcgg cccgtcctca
     3961 ggaccgggac cgggtacgga tcgccgagtc cgccgagatc tcttctctcg aaggcgagcc
     4021 ggcggccttc ttcgcctgat atcctccgcg ggcgccatgc tcgtcatcca ggacgcctac
     4081 aacagcagtg acctgcccca cggcggcgtg ggcgccatcg gcaacttcga cggcgtccac
     4141 cgcggccagc gggcgatcct cgacctggtg gtggcccgtg cccgggagct ccaggtcccc
     4201 gcggtggtca tcaccttcga cccgcacccg ctgacggtgc tgcggcccgg ccaggcaccg
     4261 acgccgctga ccacgctgcg ccagaaggag gcgctgctcg acgaggcggg ggtgtcggtg
     4321 gtgctcgtgg tccgcttcga ccaggacctg gcgcgcaccc cggcgcgcgt cttcgtgcgc
     4381 gacttcctgc acggccggct cggcctgcag gagatctacg tcggctccgg cttcgtcttc
     4441 ggccaccagc gggaggggga cctgccgctg ctgcgccaga tgggggagag cctcggcttc
     4501 aaggcgttcg gggtggagga ggtccttcaa gaaggggagc ggatctccag cacccgcgtc
     4561 cggcgcgcca tttccgaggg gaaggtgacg gaggcggccg agatgctggg gcgcccctac
     4621 acgatttccg gcatcatcgc ccgcggcgac cgcatggggc agcgcctcgg ctggccgacg
     4681 atcaacctgg tccccgagaa caagctgctg ccggctgacg gcgtctacgc cggccgggtg
     4741 ttcttcccga gctttccggc gaccttcgac tgcgtgacca acatcggcac gcggccgacg
     4801 gtctacgaga actaccagcg cgtggtcgag agccacatcc tggacttcaa gtccgatgtc
     4861 tacggcgccc gggtggacat ctccttctac aagcgattgc gggaggagag gatcttcccc
     4921 acggtgatgg acctggccgc ccagatcggc cgcgacgtcg aggccacgcg cgagtacttc
     4981 tcccggcgcc ggatggaaca agaggtgccc tccgccgggt tggagaggga gtgaggggcg
     5041 ccgtacgcct ttggccccgc cacccgagac ccgatacgat gccgtgggga acacgccctt
     5101 cgggcagccc cgacgagaaa tggagagaga gagatggcca gcgagaagat cgtgacgatc
     5161 agcgaggaca acttcgagaa cgaagtgctc aagtcggacc agccggtcct ggtggatttc
     5221 tgggcggagt ggtgcggccc ctgccggatg gtggcgccgg tgctggacca gatcgccgat
     5281 gagatggacg gcaaagtgcg catcgccaag ctgaacgtcg acgagaacca gaagatcgcc
     5341 taccagttcc aggtgtcgag catcccgacg ttcatcctgt tcaagaacgg ccaggttgcc
     5401 gaccggacca tgggcgcgat gcccaagaac gcgttccaga gcttcatcga ccggaacatc
     5461 taagagcgct ccgggccgcc cgccgcgctc cggcccccgg gcggccctgc cccgtctacg
     5521 cacatgagct cgcctgacgc cctggacggc cgtctcgcct tcctgaacgc cgggctggac
     5581 gagctgggct gccaggccct cctcgtcttc gccccctccg cggccgatcc cgacctggcg
     5641 gtcttcaccg gctccgtcca cctcggcgag tcgctgctcg tcgcgccgcg gggggagcgc
     5701 ccccggctcg cctatctcac ccccatggaa cgcgacgagg cggcggccac cggcctcctc
     5761 ctgatcaccc ccgaagagct ggatgtcctc cgctggaccc aggaggcccc cgagccggtg
     5821 gacttcacgg ccgccgtgac ggcgcgggcg ctggagctcg ccgggctctc tcccgggcgg
     5881 gtggccctgg ccgggcacgg ccgggtgggc gtcctgcacg ccgtcctctc ccgtctggcg
     5941 cgttcgggct ggtcctgggt tcccgccaac gacctgctga ccgggctgcg caaaggcaag
     6001 gtggcggggg agctggccgc catccgggcc gccgcggcgg ggaccgtcga ggccttccgc
     6061 accgtggcgc gcctgctggc cgccgccggt ctccgggcag gtgaagggga cgagctctgg
     6121 ctggaggggg agcggctcac cgtcgcccgc ctgcgcgccg aggtggcgcg gaccctcgcc
     6181 ggcttcggcc tcgagcagcc gcggggcaac atcatcgcgc cgggggagga ggggggcgtg
     6241 ccccactcga ccggcactca ggagcgggtg ctgcgcgccg gcgagtccct ggtggttgac
     6301 ctcttccccc gcggcacctt gtttgccgac tgtacccgca ccttctgtgt gggcacgccg
     6361 ccggagcccc tcgcccgcgc ccacgccgcg gtcctcgccg ccctgcgcgc ggcgcacgcc
     6421 ggagcaaccg ccggcgctac ctccacgatc aggggctggg acctccagga ggcggtctgc
     6481 cactccctcg gcgacgccgg ctatcccacc cccatctccc accccggcac cacccgcggc
     6541 tacgtccaca acctgggcca cggtgtcggc ttcgagctgc acgagcagcc gatcttcaag
     6601 aaggtcgcgg gccgggaagg ggtgctcgcc cacggcgacg tcttcaccct ggagcccggg
     6661 ctctacgatc ccgaggccgg ctacggcgtc cgcctcgagg acctcgtcca cctgaccccg
     6721 ggcggtctcg aaaacctcac gccgctgccg tacgatctcg atccccgcgc ctggggctga
     6781 aaatccggcc cgcacgctct gtccgggaac ggatcgggcc caggtccggt ccaaggaggt
     6841 gactgtatga gcgacgagac tgcggcctac gagaagctgg gagcgttcta tctggggcgc
     6901 ccgtacgacc tggagcgtgg agaagcccgg ccggtgccgc tgctctatga ttcccgcgac
     6961 ctcctgaccc acgccgtctg cgtcggcatg accggcagcg gcaagaccgg cctcctcatc
     7021 gggctgctcg aagaggctgc catcgacggc atcccctccc tggtgatcga tcccaagggg
     7081 gacctggcca acctgctgct caccttcccc cgcctcgcgc ccggcgactt tgttccctgg
     7141 gtcgacgccg acgccgccgg ccgcgcgggg atctcgcccg aggacttcgc ggcgcgcgag
     7201 gcggagacct ggaagaacgg cctcggccag tgggggcaga gcggcgagcg gatcgcgcgg
     7261 ctgcgggagg cggcggactt cgccgtctac acgccgggaa gcgagtcggg gctgccggtc
     7321 tcgatcctcg cctccctcgc ggcgcccccg gccgacgtgc tggccgacgg ggacctgctg
     7381 cgcgagaggg tctcgggcct cgccgccagc ctcctcaccc tgctggggat cgagggggac
     7441 gacgccgacc cggtgcgcgg gcgcgaccac atcctgctct cgaccctgtt cgacgccgcc
     7501 tggcgggagg ggcgcggcct cgacctcgcg acgctcatcc agcagatcca gaagccgccg
     7561 gtggagcgcg tcggcgtcct cgacctcgaa tcgttctatc ccgccaagga gcgcttcgct
     7621 ctggccatgg cggtcaacaa cctgctgggc tctcccggct tcgcggcctg gctgcgcggc
     7681 gagccgctgg acgtcgaccg gctgctctac accccctccg gcaagccgcg ggtggccgtc
     7741 ttctcgatcg cccacctggg ggaccgggag cggatgttct tcgtctcgct gctcctccag
     7801 cagaccctcg cctggatgcg gtccaagccg ggcacctcca gcctgcgggc gatcctctgc
     7861 atggacgagg tcttcggcta catgccgccg gtggccgagc cgccgtcgaa gcgggcgatc
     7921 ctcaccctgc tcaagcaggc ccgcgccttc ggcctcggcc tggtgctcgc cacccagaat
     7981 ccggtcgacc tcgactacaa ggggctgggc aacgtcggca cctggtttct cggacggctg
     8041 cagaccgagc gcgacaagca gcgcctgctc gacggcctcc agggggctgc gggcggcatg
     8101 gaccgcggcg agctggagcg gatgctgtcg cagctcggca agcgggtgtt cctgctccac
     8161 aacgtccatg aggaggagcc ggaggtcttc cagacccgct ggacgctgtc gtacctccgc
     8221 gggccgctca cccgcaccga gatcaagcgg ctgatggacc cgcgcaaggg gggcacgccc
     8281 gcagccacgg cgacggctcc agccccgtcc gccccgtccg ccgcctcgga tagcggcacc
     8341 ggcgcgccgc cggtgctgcc gcccgatatc tcccagcgct tcctgccggt ttgctcgcgg
     8401 ccgtccggca tcgtctaccg tccggccctc ttcgccgcgg ccaaggtgca cttcgccgac
     8461 gccaaacagg ggatcgagca cgaggaggag acggcgctgc tcgctctcat ggcagcgggg
     8521 gaaggggagc cggtggactg gtacgccgcc gagggcgtcg agttgtggaa agaagacctg
     8581 gagcccgagc cggtggcggg ggcgcgcttc gagccgctgc ccgacgccgc ggtgcgggcc
     8641 aagagctacg acggctggcg gcgggacctc gaggagtgcc tttaccggac ccgccgctgc
     8701 gacctgttcc gcagcccggc cctgggggtg acctctcgcc ccggcgagct ggagcgcgac
     8761 ttccgtatcc gcctgtccga cttggcgcgc gagaagcggg acgggcaggt agagactctg
     8821 cgcaagaagt acgcgtccaa ggtggcgcag ctcgaggacc gcatccgccg ggccgagcag
     8881 accaaggagc gtcaggagga ccaggtccgg cagcagggtt ggcagaccgt cctctcggcc
     8941 ggcacggcgg tggcggcaat gctcttcggc cgcaagaagc tgagccacgc gaacctcggc
     9001 cgcgccacct cggcggtgcg cggcgtcggc cggactttcc aggagcgcca ggacgtgagc
     9061 cgggcccagg agaacgtcga ggcgctccag cgccagctcg ccgacctgaa caccgagctc
     9121 gaggcggaga tcgggaagct cgaggcccgc ttcgatcccg aggcggagcc gctcgagacc
     9181 gtgagcctca agccgcggcg caccgacgtc gaggtccgct tcctctccct ggcctgggct
     9241 ccgggccgtc ctggagacgc cggctgggag ccggcctggc ggtgagcggc ccgtgcaggg
     9301 agaaaccgtg gctcgccgcc ctcgtagaca gggttgggag gagtaaagag gcacgatggt
     9361 acgttttgat tctcaggact cggggcgcgt ggcaagggtg tgtaacttct atacggcggc
     9421 tcgcggccgc cggctgacgc agaaggagcg cggcattgga agtctttctc agcattctgg
     9481 tcggcctggg cctgagcgcg gcctgcggat tccgtatctt cattcctcct ctgatcctct
     9541 cgctcgccgc gcgcggcggg catctggagc tcggcgacag cttcgcgtgg atgggctcga
     9601 acgcggcgct gatctcgttc gcggtggcca ccgtgctgga gattgccggg tattacatcc
     9661 cctgggtgga caacctgctc gacgccatcg ccacgcccac ggcggtggtg gccgggattc
     9721 tggtttcggc ctcgacgttt accgacgtga ccccgttcct caagtggacg ctggcgatcc
     9781 tcgccggcgg cgggtcggcg gcggtgttcc aggggatcac ggccacggcg cggcagatct
     9841 cgtcgctcag cacgggtggc ctgggcaacc cgctccttgc cacggtcgag gcggcgggct
     9901 cggcgttgtt ggcggtcctt tccgtgacgt tgccgttgtt ggcgatcctg gtggtggtcg
     9961 gtctgttgta ctttggagtg aggcgtctac tttttcgccg tccagtgcgg gtcacttgag
    10021 ggagagatcc cgcgcccggc gttcaggaag tcaggaggaa tgaagctcat gctcgacttc
    10081 aagagacgat cccgcgtccg gcacacctct ctgggcgcca cgctcctcgc tgtggctggg
    10141 gcgatggcgc tcgggggctg tgccaccggt ggcccgcctg atcaggggga tcgctcgcag
    10201 atcgctccca cgctgcaggc ccggggcgtc gaccctaacg ccgtggtcat cccctatgag
    10261 ctgaccgacg agatgcgcga gtgggtgcac cgcatggtgc cgcggaacct ctcgttcgat
    10321 cggcgcctgc cgatgctggt cgacgccctg ctcagcgaga agggtctcaa tgtcacgtac
    10381 gaggcgaagc acaccggcac ggcggaggag gtcttcaaga cgcgccgcgc caactgcctc
    10441 gccttcacca gcctgttcgt ggcgatgtcg cgcgaggtgg gggtgccggc cttcttcctc
    10501 gacgtccagg agctggagcg gttcgagaag gagggagacc tggtggtggt ctcgggtcac
    10561 gtcagcgccg gctatgggat cggcaagacc ttgcaggtcc tggacttctc gtccgtgtct
    10621 ccgacgtcgg gctaccagcg ccgggtgttc cccatttccg acgtccgggc gatcgcgctc
    10681 tatcactcca accgtggcgc cgaggcgcta cgcgccggca acgaggagga ggcgctggac
    10741 tggctgcaca aggcggtggc catcgatccc gaggtgccgg gcgcctggat caactatggc
    10801 gtggcgctgc ggcgctctgg caaggacggc gaggcggaga acgcctaccg caaggcgctg
    10861 gagatcgact cgggcgcggt gtcggcctat cagaacctgg cgtcgctgct gcgctacaag
    10921 gggcggacgc aggaggccga gcagctcatg gcgctgactc tccggatggg cacccgcaac
    10981 ccgtacaatt acctggcgct cggcgacctg agcctgtctc acggccgttt cgacgaggcc
    11041 cgccggtact acaagcgggc catgcggctc taccgggaca atccggagcc ctacgccgcc
    11101 ctggggatca tctcgctgat cgatggcgac cggttggagg ccaccaagtg gctgcgcaaa
    11161 gccagcgcca tcgaccagga gaacaaccgg gtcaagcagc tccaggcccg tctcggcagc
    11221 caggtcgggt gagcggacag gcatgaggtc cctcacccgc catctcacga tgaacgtccc
    11281 ggcgcgtatg gacttcgtca acatcacgcc ggacgtggag cgtgccgtgg cggagagcgg
    11341 ggtccaggag gggctctgtc tggtcaacgc catgcacatc acggcgagcg tcttcatcaa
    11401 cgacgacgag ccggggctgc accgggatta caagcgctgg ctcgaggagc tggcgccgtt
    11461 cgacgccagc tcgcagcgct accatcacaa ccgcaccggt gaggacaacg ccgacgccca
    11521 ccacaagcgc cagatcatgg gccgcgaggt ggtggtggcg atcaccggcg gccgtctcga
    11581 cttcggtccc tgggagcaga tcttctacgg ggagttcgac ggccgccggc ccaagcgggt
    11641 gctggtgaag gtcatcgggg agtaggggcg gccagcgtgg ccggcggctg gcgcatctgg
    11701 gtcgacaccg gagggacgtt caccgattgc ctggcggtgg acccctccgg cgggctccac
    11761 cgtgccaagg tgctgtccac cagcgccttg cgcggacggg tggaggagcg ggccggcccc
    11821 acctccctcc ggctcgccgc cccctggcgg gtccccgcgg acttcttcgc cggcttcact
    11881 ttcagactcc tcggcggcgg cgcctcccag gctgccgccg tgacggggta cgacccggcc
    11941 ggcgttctcg atctggcagc tcccatcgac ggcagcctct ccggcgcgac cttcgagctg
    12001 cgctccccgg aggaggcccc ggtcctcgcc gcccgtctgg tgaccggaac tccactcggc
    12061 tgggcgctgc cggagatcgc cctgcgcctc gccacgaccc gggggaccaa tgccctcctg
    12121 gagcgcaagg gggcggccac cgcgctgttc gtcacccgcg gcttcgccga cctgctgcgc
    12181 atcggtacgc agcagcgtcc cgatctgttc gccttggaag tccgccggcc cgagcccctc
    12241 tatcgcgagg cggtggaggt tcccgagagg ctggccgcgg acggctccgt cgtcgagccc
    12301 ctcgatctcg agaccctgcg cgccacggcc gagcggctgc tggcggatgg ggtcgtgacg
    12361 gcggccgtgg cgctgatgca cggctaccgc aatccggccc acgaagagga gctgggcgcc
    12421 ttcctcgcag ggctcggttt ccggcacgtc tccctctcct cgcgcctggc cccgctgatc
    12481 aagctgctcc cccgggccga gaccgcggtg gtggacgcct acctgtcccc gctgctggcg
    12541 gattacctgg ccggggtctc cagagcgatc tcgcacggct cactgcacgt gatgaccagc
    12601 gccggcggct tggtgcgggc cgaggcgttc cgcgccaagg acgccctgct ctccgggccc
    12661 gccggcggag tggtgggggc ggctctctcc gggcggcggt cggggttccg gcgggtgatc
    12721 gcgttcgaca tgggcggcac tagcaccgac gtcgcccgct gggacggcga tttcgagtac
    12781 ctcttcgagc accgggtggg ggacgcccat ctggtggcac cggccttggc catcgagagc
    12841 gtggccgccg gcggcggctc gatctgctcc ttcgacggcg cccagctccg ggtggggccc
    12901 gagagcgccg gcgctgctcc cgggcctgcc tgctacggtg ccggcggacc gctgacgctc
    12961 accgacgtca acctcctcct gggacggctg gatccaggca ggttcgagat cccgctcgat
    13021 cccggggcgg cggagcgggc gctcggagag ctcctggagg ggatggcggc gcgcgggccg
    13081 gcacccgagc cggaggccgt tctggcgggg ctgctggaca tcgccaacga gaagatggcc
    13141 gaggcgatcc gggaggtgtc ggtccgccgt ggctacgacc ccgcggacca cgctctcgtc
    13201 gccttcggcg gtgccggagc ccagcacgca tgcgccgtgg ccgagctgct ggggatcggc
    13261 agcgttctgg tgccggtgga cgccggtctg ctctcggctc tcggcctggg ggcggcggtg
    13321 gtggagcgct tcgcccagcg gcaggtgctc gcgcccctgg tccaggtgga agggcacctg
    13381 caggggtggc tcgacgagct ggggcgtcag gcggtctccg acgtcgtggc cgagggcatg
    13441 cttccggagt ctgtccgggt ccgccgtcgg ctgctcaacc tccgcttcac cggccaggag
    13501 acggcgctga cggtggagta tggcgaggag cccgtggacc aggccttcgc tgccgcgtac
    13561 cgagagatct acggctatgc tcccgaggga cgggcggtgg agctggagtc ggtgcgggtg
    13621 gtggcctcgt cgtccgcgga ggaggaggtc ttgccggcgg aggcggcgac gcggttcccc
    13681 gcggcgcctg agggctcccg gcgctgctgc ttcggtggaa gctggcggga cgtccctttc
    13741 ttcgaccgag cggggctccg tcccggcgcc tccttccagg gaccggccct ggtcttcgag
    13801 gcgcacagcg ccacggtggt ggccgagggc tgggacggga tggcggacgg cgccggcggc
    13861 ctggtcctgg cgcagcggta gcggatctgg aggatgggca gagtgcagga aaattggaat
    13921 ctgatccagc gggaagcgtt ctttgctcag cggctgcagc cgctgcggcg gatctgcgcc
    13981 gccctggccg cgaacgctgt gctcatggcc gcggtagcct ggttcctcct cgggcccggg
    14041 ggcttcgtgc cgccgcgcct gccggacgcg gtgccgctct ccatcacggc ggcctcggcg
//
LOCUS       DASPJI010000281         2890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_250393, whole genome shotgun sequence.
ACCESSION   DASPJI010000281 DASPJI010000000
VERSION     DASPJI010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250393"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VEL74_07470"
     CDS             <1..402
                     /locus_tag="VEL74_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HYG62404.1"
                     /translation="RGIVEARRVLADRLRRGEEIPGFGQPLYPEGDPRGKALLALAAE
                     VAPGSKEVELAEALAEAGRELLGEHPTLDLGLVAVARALGLAERTPLALFALGRAIGW
                     LGHAIEQYAAGRLIRPRATYVGEPPEGELSV"
     gene            complement(489..1727)
                     /locus_tag="VEL74_07475"
     CDS             complement(489..1727)
                     /locus_tag="VEL74_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HYG62405.1"
                     /translation="MTKQWLRAVGTAAVLTLVAVSAQASPSLFSPLDVVSFGKALSLD
                     PSYDALVNDPATGNLHLVQADAAALTDKTATVTLGLAPGYEVRAHQVESFRSTSGNLI
                     WSGVIEDPGAVTVPFSPQALRFDPINSVVLVKDGDQIDGNVHVGGHWYAIRSLKSGGH
                     AIVEMNMAAMPPDHPENAKLPVRRMPATPGMNKASTIRVMVHYTSSAASAAGNISSLI
                     DLAITESNIGYANSGVGITLQLATKAQITYTQSGSFSTDLSRYRGTSDGYMDSVHTTR
                     NSVAADVGVLVINNSSSCGLASGIGSTASTAFAAAHWSCITGYYSFAHEIGHLLSARH
                     DPATDPSTSPYRYGHGYRYTGSPDWRTIMAYDCPGGCPRLNYWSSPLKLYNGVPMGNS
                     TQSDNARVLNARAATVAAFR"
     gene            1932..2270
                     /locus_tag="VEL74_07480"
     CDS             1932..2270
                     /locus_tag="VEL74_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62406.1"
                     /translation="MSIEQSPDFDGEYGAVQLPEAWHVLSLVCFAGALGSRFLVILIP
                     EVLRSMVHRSWLPGIVTLWLAAIGLALGLRGLRVPAGRSTARVAIFLNAIVVALCLLA
                     GAAVVYIMPG"
     gene            2332..2520
                     /locus_tag="VEL74_07485"
     CDS             2332..2520
                     /locus_tag="VEL74_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62407.1"
                     /translation="MNRKLKRLALHRETVRNLSETEVRTVAGGITETDCIRVCSIENC
                     RTDTCTQCSNTCRVYTEG"
     gene            complement(2517..>2890)
                     /locus_tag="VEL74_07490"
     CDS             complement(2517..>2890)
                     /locus_tag="VEL74_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62408.1"
                     /translation="YHLEAGIAACHAIAPTWEETDWPLILSHYDELLARKPSPIVALS
                     RAVAVAQVHGPDAGLAALEPVKGHPSLAAYYPLPAVEAELSERAGDPARAAASYRRAL
                     DLPCPAPVRRRMQERLRRLEA"
BASE COUNT          432 a          911 c         1079 g          468 t
ORIGIN      
        1 cgtgggatcg tggaggcgcg gcgggtgctg gccgaccggc tgcggcgggg cgaggagatc
       61 ccgggcttcg ggcagccgct ctatccggag ggagacccgc gcggcaaggc cctcctcgcc
      121 ctggcggccg aggtggcccc aggctcgaag gaggtcgagc tggcggaggc gctggccgag
      181 gccggccgcg agctcctcgg cgagcatccc accctcgacc tcgggctggt ggccgtggca
      241 cgggccctcg gcctggccga acgcacgccg ctcgccctct tcgccctcgg ccgcgccatc
      301 ggctggctcg gccacgcgat cgagcagtac gccgcgggac ggctgatccg tccgcgcgcc
      361 acctatgtcg gggaaccgcc tgagggggag ctgtccgtgt aggtccgtgc aggtccgtgg
      421 aagtccgtga gtccgtgtaa cgcccccggg gggcgggtgc caggcacccg ccccgggatt
      481 cgaaaacctt accggaaggc ggcgacggtc gcggcgcggg cgttcagcac gcgggcgttg
      541 tcgctctgcg tggagttgcc catgggcacg ccgttgtaga gcttgagcgg gctcgaccag
      601 tagttgaggc gcgggcagcc gccggggcag tcgtaggcca tgatcgtgcg ccagtcgggg
      661 ctgccggtgt accggtagcc gtggccgtag cggtacggcg aggtcgaggg gtcggtcgcc
      721 gggtcgtggc gggcggagag gaggtgcccg atctcatggg cgaaggagta gtagccggtg
      781 atgcagctcc agtgggcggc ggcgaacgcc gtcgaggcgg tggagccgat gcccgaggcc
      841 aggccgcagg aagagctgtt gttgatcacc agcacgccga cgtcggcggc caccgagttg
      901 cgggtggtgt ggacgctgtc catgtagccg tcgctggtgc cgcggtagcg gctcaggtcg
      961 gtgctgaagc tgccgctctg cgtgtaggtg atctgcgcct tggtggcgag ctgcagggtg
     1021 atgccgacgc cgctgttcgc gtagccgatg ttcgactcgg tgatggcgag atcgatcagg
     1081 ctgctgatgt tgcccgcggc gctggcggcg gaggaggtgt agtggaccat gacgcggatc
     1141 gtcgaggcct tgttcatgcc gggcgtggcg ggcatccgcc ggacggggag cttggcgttc
     1201 tccggatggt cgggaggcat ggccgccatg ttcatctcga cgatcgcatg gccgcccgac
     1261 ttgagcgaac ggatcgcgta ccagtggccg cccacgtgga cgttgccatc gatctgatca
     1321 ccgtccttga ccaggaccac cgaattgatc ggatcgaacc gcagcgcctg cggggagaag
     1381 ggcacggtca ccgcgcccgg gtcctcgatg acaccggacc agatcaggtt gccggacgtg
     1441 gagcggaagg actccacctg atgcgcgcgc acctcgtagc ccggcgcgag ccccagggtc
     1501 accgtcgccg tcttgtcggt cagggccgcc gcgtcggcct gcaccagatg caggttgccg
     1561 gtggcgggat cgttgaccag cgcgtcatac gacggatcga ggctcagcgc cttgccaaag
     1621 gaaacgacat ccagaggcga gaacagagac ggactcgcct gggcggagac tgcgaccaac
     1681 gtgaggacgg ccgccgttcc aacagcacgc aaccactgct tcgtcattta caacctcctg
     1741 atgaataaaa atagacacat agctacgctg gtactcaacg ccgtggcgac actgcgccgc
     1801 ctgatgcgct cccgggaagc gccgttacct gcagggaccc ctttgatgag tacggtgctc
     1861 gggagtgaaa aaagcgcgcc ggccgttttc atctcctttg agctagaatc actgctctct
     1921 gaggtctgat cgtgagcatc gagcaatcgc ccgacttcga tggcgaatat ggcgccgtcc
     1981 aactgccgga ggcctggcac gttctgtcgc tcgtctgctt cgccggagcc ctgggatccc
     2041 ggtttctggt gatcctgatt cccgaagtat tgaggtcgat ggtgcaccgc tcctggctgc
     2101 cggggatcgt gaccctgtgg ctggcggcga tcggcctggc gctggggctg aggggactgc
     2161 gggttccggc ggggcggtcc accgcgcggg tggcgatctt cctgaacgcg atcgtcgtgg
     2221 ccctctgcct cctggcgggg gcggccgtcg tctacatcat gccgggctga gcgctcgctt
     2281 tttctccggc cgggcacctc tcttcatgaa ggcccgaaag gaggaacggt catgaaccgg
     2341 aagctcaaga ggctggcgct ccaccgcgag acggtgcgga acctctccga gaccgaggtg
     2401 cggacggtcg ccggcggcat cacggagacg gactgcatcc gggtgtgcag catcgagaac
     2461 tgccgcaccg acacctgcac gcagtgctcg aacacctgcc gcgtctacac ggagggttag
     2521 gcttccagcc ggcgcagcct ctcctgcatc cgccgccgca ccggcgcggg gcaggggaga
     2581 tcgagcgccc ggcgatagct cgcggccgcg cgggccgggt caccggcgcg ttcggacagc
     2641 tccgcctcca ccgccggcag cgggtagtag gcggcgaggg acgggtggcc cttgaccggt
     2701 tccagggcgg cgaggccggc gtccgggccg tggacctggg ccacggccac ggcccggctc
     2761 agggccacga tgggggaggg cttccgggcc agcagctcgt catagtgcga gaggatcagc
     2821 ggccagtcgg tctcctccca ggtgggggcg atggcgtggc aggcggcgat accggcttcc
     2881 aggtggtagt
//
LOCUS       DASPJI010000282         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_310771, whole genome shotgun sequence.
ACCESSION   DASPJI010000282 DASPJI010000000
VERSION     DASPJI010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310771"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2387)
                     /locus_tag="VEL74_07495"
     CDS             complement(<1..2387)
                     /locus_tag="VEL74_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG62409.1"
                     /translation="MDTFLQDLRFACRTLLKRPVPTTVAVLTLALGIGANTAIFSVVD
                     AVLLSPLPFREPERLAVIWASNPDLAAKVGLPDNLPASPGTFQDWKRDARSFESMAMV
                     GADRLALTGKGEPELLGAVQVSGDFFTVLGSRPLLGRTLLPEDDLLGKRTTVLLSQGL
                     WQRRFGGDPEIVGRKVLLDGEPLTVVGVMPAAFNFPRGAQFPFGYGFTEAPDVWVPMG
                     FSAEDRENRDNHSHLTIARLKPGVGLAAAREEMNAITRRLQATYPNDVGWGARPESLA
                     GQLVGPVRPALLVLLGAVGLVLLIACANVANLFLAQAVTRQKEISIRTAMGAGRGRLA
                     RQLLTESLLLAVVGGALGLLAAFWGLRWIASWIPASMPVPAEISLDARVLLFTLAAVL
                     LAGLLSGLVPALQTTRTDLSETLREGNRGAGSAAPSRRMRSALVIAEVALAVLLVIGA
                     GLLIRSFARLTAVDPGFRADNVLTLQVILPGSKYNRDQRAPFFASVLERLRALPGVEA
                     AGAVSNLPLSGEESVGAIVIEGRPRPEPNEIALADQRSIMPGYFEALGIALVRGRTLE
                     ERDLTYGEDGPRMAVIDETMAKAYWPGEDPLGRRFRLGFYDDTDANWFTVVGVVRNVR
                     HSGLHVDPRPQMYLPESRFPSGQMTLVLRTAGNPEALTDEARAAVYAVDRDQPVSRVG
                     TMERMVGESLAGRRFTMILLVVFAGLALALAAVGIYGITSYSVTQRTREMGLRMALGA
                     RPGQVVRLVVQEAGTLAVLGIVLGLGLGFAATRVMSSLLFGVKATDPGTYVAVSVA"
     gene            2565..>2777
                     /locus_tag="VEL74_07500"
     CDS             2565..>2777
                     /locus_tag="VEL74_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYG62410.1"
                     /translation="MSLDQIDRRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGAVKA
                     RGEARRARSDWMKVEQERGISVTASVM"
BASE COUNT          445 a          986 c          991 g          355 t
ORIGIN      
        1 gccaccgaca ccgccacgta ggtccccggg tccgtggcct tgaccccgaa gagcaggctg
       61 gacatgaccc gcgtggcggc gaagcccagg ccgagaccca gaacgatgcc gagcaccgcc
      121 agagtccccg cctcctgcac caccagccgc accacctgcc cgggccgcgc ccccagggcc
      181 atgcgcagcc ccatctcccg ggtgcgctgc gtcaccgagt aggaggtgat gccgtagatg
      241 cccaccgccg ccagcgccag ggccaggccg gcgaagacca cgagcaggat catggtgaag
      301 cggcggccgg ccagcgactc ccccaccatc cgttccatgg tgccgacgcg ggagaccggc
      361 tggtcccggt cgaccgcgta gaccgcggcg cgggcctcgt ccgtcagcgc ctcggggttg
      421 ccggcggtgc gcagcaccag cgtcatctgc cccgacggga agcggctctc gggcaggtac
      481 atctgcggcc gcggatcgac gtgcaggccc gagtggcgga cgttgcggac cacgccgacg
      541 accgtgaacc agttggcgtc ggtgtcgtcg tagaagccga gccggaagcg cctgcccagg
      601 ggatcctcgc cgggccagta ggccttggcc atggtctcgt cgatcaccgc catccgcggc
      661 ccgtcctctc cgtaggtcag atcgcgctcc tccagggtcc tcccccggac gagggcgatc
      721 cccaaggctt cgaaatagcc cggcatgatg gagcgctgat ccgccagggc gatctcgttg
      781 ggctcgggac ggggccgtcc ctcgatgacg atggcgccga cgctctcctc accgctcaag
      841 gggaggttcg acacggcgcc cgccgcctcg acgcccggca aggcccgcag ccgctccagc
      901 acggaggcaa agaagggcgc gcgctgatca cggttgtact tggagccggg caggatgacc
      961 tgcagggtga gcacgttgtc cgcccggaag ccgggatcga ccgcggtcag gcgcgcgaag
     1021 ctgcggatca ggagcccggc gccgatgacc agcagcacgg cgagcgccac ttcggcgatg
     1081 accagcgcgc tccgcatgcg gcggctcggc gcggcgctcc cggcgccgcg gttgccctcg
     1141 cggagcgtct cggagaggtc ggtccgggtg gtctggaggg cgggcacgag gccggagagg
     1201 agccccgcca ggaggaccgc cgccagggtg aagaggagga cgcgggcgtc gagcgagatc
     1261 tccgccggca ccggcatgct cgccgggatc caggaggcga tccagcgcag cccccagaag
     1321 gcggcgagga ggcccagcgc gccgcccacc accgccagca gcaggctctc ggtcaggagc
     1381 tgccgcgcca gccggcctct ccccgccccc atcgccgtgc ggatggagat ctccttctgc
     1441 cgcgtcacgg cctgggcgag gaacaggttg gccacgttgg cgcaggcgat gagcagcacc
     1501 agccccaccg ctcccagcag caccagcagc gccggccgca ccgggccgac gagctggccg
     1561 gcgagcgact cggggcgcgc cccccagccc acgtcgttgg gataggtcgc ctggagccgc
     1621 ctggtgatcg cgttcatctc ctcccgtgcg gcggcgaggc cgacgccggg cttcagccgg
     1681 gcgatggtga gatggctgtg gttgtcccgg ttctcgcggt cctcggcgga gaagcccatg
     1741 gggacccaga cgtccggggc ctcggtgaag ccgtagccga aggggaactg cgcgccgcgc
     1801 gggaagttga aggcggcagg catgacgccg accacggtca gcggctcgcc atccagcagc
     1861 accttccggc cgacgatctc cggatcgccg ccgaaccggc gctgccacaa cccctgggag
     1921 agcagcaccg tggtccgctt ccccagcagg tcgtcctcgg gcagcagggt gcggccgagg
     1981 agcggacggc tccccagcac cgtaaagaaa tcgcccgaca cctggaccgc tcccaggagc
     2041 tccggctccc ccttgccggt cagcgcgagg cggtcggcgc cgaccatggc catggactcg
     2101 aacgagcggg catcccgctt ccagtcctgg aacgtccccg gcgaggccgg caggttgtcg
     2161 gggagcccca ccttggcggc gaggtccggg ttggaggccc agatcacggc cagccgttcc
     2221 ggctcacgga acggcagcgg cgagagcagc acggcgtcga cgacactgaa gatcgcggta
     2281 ttggcgccga tgcccagcgc cagagtcaga acggcgaccg tcgtggggac cggccgcttg
     2341 agaagagtcc ggcaggcgaa tcgaaggtct tgaaggaagg tgtccatgac gtttctccct
     2401 gcgtcgtgga ctcctacgcg gcgggagggg ggagggtttc aggggcggga gcgctctccg
     2461 ggaatctgct accctatccg cccgtttgac attgtctgac acaagcgggc aggcacgagc
     2521 ggctccccgc ctctccctcc agcagactgt ttaaaaggac aggtatgtct ctcgatcaga
     2581 tcgaccgcag gcggacgttc gccatcatct cccacccgga cgcgggcaag acgacgctga
     2641 ccgagaagct gctgctcttc ggcggcgcca tccagatggc cggcgcggtc aaggcgcgcg
     2701 gcgaggcccg gcgcgcccgt tcggactgga tgaaggtgga gcaggagcgc ggcatctcgg
     2761 tgaccgcctc ggtcatg
//
LOCUS       DASPJI010000283         9697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_761527, whole genome shotgun sequence.
ACCESSION   DASPJI010000283 DASPJI010000000
VERSION     DASPJI010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9697)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9697
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761527"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VEL74_07505"
     CDS             <1..206
                     /locus_tag="VEL74_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62411.1"
                     /translation="DLDPLRRFERSWPGLGGELDALLEQPPPRAASGLLALARRELAG
                     RLPGYPAAAMETLERVVSEAGEA"
     gene            203..772
                     /locus_tag="VEL74_07510"
     CDS             203..772
                     /locus_tag="VEL74_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62412.1"
                     /translation="MSDKPEISESEAVILCVQDVWMAIGLRWSAIRRELAAFPFPPGT
                     LDGGRAPFELMLAAMGLQIQSAESLLSTGQARRVRDLLLTHFVSEHGRGALSVLEAHD
                     DAWREALAGGHGAALGPAQVMAERLGCAPTDEAGPSGAGRAPGSPVLRGLAGVLERYG
                     TPDWWQRLLASSRLVPDADMPDADASSAF"
     gene            824..1765
                     /locus_tag="VEL74_07515"
     CDS             824..1765
                     /locus_tag="VEL74_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG62413.1"
                     /translation="MTTAPPTSATALRCRGLVKRYADVVAVNGLDLEVVRGECFGLLG
                     PNGAGKTTTIEILEGLTQPDAGEVEVLGQTWQGEGHHLRERLGVSLQETQLNEKLTII
                     ETLRLFHSFYRNGRDPEQVLASLALEEKRDARVGKLSGGQKQRLAVACALVGNPDLLF
                     LDEPTTGLDPQSRLQLWEQVTTFQNGGGTVLLTTHYMEEAERLCDRVAIVDHGKVIAL
                     GTPDELIRSLHAADVIEFLSEPLLPEEELRRLPGVSDVHRRGPNWALAVSSVTESVPA
                     LLARVEAAGARLSYLSTHRATLEDLFVSLTGRELRDA"
     gene            1758..2807
                     /locus_tag="VEL74_07520"
     CDS             1758..2807
                     /locus_tag="VEL74_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG62414.1"
                     /translation="MPEPSSSRLRALRELTRCRILEFTREPEAIFWVFIFPALLAAVL
                     GFAFREKPPDRIPVGVVAGPAAEQTQAALAKSPVLLAREYETQAGREALRTGKISLLV
                     EPGPPVVFRFDETRPDSRIARLEADDALQRAAGRGDPLQVRSERVTEKGSRYIDFLVP
                     GLLGMNLMGTGIWSLAFSITSARNRRILKRLVATPMRRGDYLLSQILGRLVFLIPEVV
                     VLVGIGALVFGVPLRGSILLLGFVCLLAAMSFCGLGLLIASRVRTIEGASGLANFVML
                     PMWLLSGVFFSSERFPDMIQPLVRALPLTAVNEALRAIMNEGQGLVGVLPQLAIVAAW
                     GLVSFVVALKIFRWQ"
     gene            complement(2986..3747)
                     /gene="larB"
                     /locus_tag="VEL74_07525"
     CDS             complement(2986..3747)
                     /gene="larB"
                     /locus_tag="VEL74_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HYG62415.1"
                     /translation="MNPHQIKEILDAVAAGALTPEAALEDLAQLPYADLGFAKLDLHR
                     ELRNGLPETVYGEGKRTDDLRVIVERMMEANGRVLVTRIRQETADALQGVFPSAVYHE
                     RARVLTCGEPHEKAGGPVAVLAAGTSDLPVAEEAAVCAAWFGFEVDRHYDVGVAGLHR
                     MLSRLPDIRKASVVIAVAGMDGALPTVVASLVPAPVVAVPTSVGYGASFGGLAALLTM
                     LNACAPGIGVVNIDNGYGAAVLASRIARMARTQRS"
     gene            complement(3744..4559)
                     /gene="larE"
                     /locus_tag="VEL74_07530"
     CDS             complement(3744..4559)
                     /gene="larE"
                     /locus_tag="VEL74_07530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HYG62416.1"
                     /translation="MDLSLRIDTVIADVVTDLAGRGSVLVAYSGGVDSAVVAALAFRA
                     VGERAVAVTAAAETLAGAELDHARRLAAEIGIRHETVTYSELDDPEFVANPSHRCYVC
                     QGLRMSTMVRLAAERGYAVVCDGTNASDPGPDRPGLKAVRESGVYSPLLEHGIDKAAT
                     RAIASALGLSVWDRPANACLSSRIPHGQLVTLGKLRRIEAAEELLAEHGFRVVRVRHD
                     QGAARVEVAPDEVERLAGIWNDVEPRLRSLGFERVAYDPHGYRRGGADLPVTP"
     gene            complement(4603..4980)
                     /locus_tag="VEL74_07535"
     CDS             complement(4603..4980)
                     /locus_tag="VEL74_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62417.1"
                     /translation="MTRLETVLFGLFLACWVVDLLVFLRMVTVAGSLALGFYPLYSAA
                     MSLGWISGYFYERRRADFPDPLRRRVFLLYFVGPPGLIYLLRAMAPFEMQAAAPLVTL
                     YAFGVFAIFFWVPIKFPLRRLPK"
     gene            complement(4977..7931)
                     /gene="gcvP"
                     /locus_tag="VEL74_07540"
     CDS             complement(4977..7931)
                     /gene="gcvP"
                     /locus_tag="VEL74_07540"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HYG62418.1"
                     /translation="MAKPEPSILDPLAPVDTFVRRHIGPNESEAREMLASLGLDSFEG
                     LVRETVPESIRLRGGLRLAELPEDRTLGERETLDALRRLADSNQVFRSFLGMGYHDCL
                     VPTVIQRNVLENPGWYTQYTPYQAEISQGRLEALLNFQTMVADLTGLPVANASLLDEA
                     TAAAEAMHMCRALTDEDRPAFWVAEDCHPQTLAVVRTRAEAIGVEVRVGRPEAPDLDG
                     VFAILLQYPATDGRVVDYEPLVRRAHEAKVLVVMAADLLSLALLRPPGEIGADIAVGS
                     AQRFGVPLGYGGPHAAFLATSEQYKRQLPGRIIGVSRDRRGHTALRMAMQTREQHIRR
                     EKATSNICTAQVLLAVMAGFYAVYHGPEGLARIARRVHALAAVLAEGLRRLGHEAGRE
                     PFFDTLRVEVKGRSASEIIAAAAARRINLRPLGESTVGIALDEAATAADLDALFAVFG
                     GDQAPGFKAAELASGIDSDIPAPHARTSPYLTHPVFHRYHTEHEMLRYIKRLEGRDLS
                     LTTSMIPLGSCTMKLNATTEMIPITWAGFAGIHPFAPVEQTRGYQELFRTLEQWLVEI
                     TGFAACSLQPNAGSQGEYAGLMVIRAYHRQEGRERGQGERDVCLIPVSAHGTNPASAV
                     MAGLKVVPVACDASGNVELDDLRAKAEAHAGRLAALMVTYPSTHGVFEEEIREICDIV
                     HRHGGLVYLDGANMNAQVGLCRPGDYGADVCHLNLHKTFCIPHGGGGPGMGPICVAEH
                     LAPFLPGHPVVHVGGGEGMGAVSAAPWGSAAILPISWAYIAMMGAQGLTRASEVAILN
                     ANYMAERLKPYFKVLYSGRRGRVAHEFILDLRPFKASAGVEAVDVAKRLMDYGFHAPT
                     MSFPVAGTLMIEPTESESRAELDRLCDGLIAIRDEIRAIEEGRADRKENPLKYAPHTL
                     EDVTESGWDRPYSREQAAFPAAWVRERKFWPYVGRVDDAWGDRNLFCTCPPMEDYAEP
                     VDAEALEVGAAR"
     gene            complement(7918..8304)
                     /gene="gcvH"
                     /locus_tag="VEL74_07545"
     CDS             complement(7918..8304)
                     /gene="gcvH"
                     /locus_tag="VEL74_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HYG62419.1"
                     /translation="MYPSEYLYSREHEWVRVDDDVCTVGITEFAQQELGEVVFVELPE
                     AGQVFNSGDEIGTIESVKAVAEVYTPVAGEIVEVNDAVVDDPELLNEDPQSEGWLIKI
                     RFSSAADLKSLMKAEEYEEYVKSGEA"
     gene            complement(8501..9619)
                     /gene="gcvT"
                     /locus_tag="VEL74_07550"
     CDS             complement(8501..9619)
                     /gene="gcvT"
                     /locus_tag="VEL74_07550"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HYG62420.1"
                     /translation="MSESSESTESLRRTPLHECHVEQGARLVPFAGWEMPVQYAGVID
                     EHRAVRTAAGLFDVSHMGEVRVRGAGAEALLQRLTPNDVSKLVPGRAHYSGLLTEQGT
                     YIDDVLVYKLADGDYMVVVNASNAARDFEWIASRVDSAEDVEVIDESDRYALLALQGP
                     KAVEILTPLATPGAAATRYYGFLQGEVAGRPALISRTGYTGEDGFELYVDPDDAPEIW
                     RRLVAAGAVPAGLGARDTLRLEAAMALYGHEIDETTTPWEAGLGWVVKLDKGEFLGRD
                     ALAAQRHDGLRRKLVGFEVDGRGIARQGHGVLSSAGGEKVGDVTSGTWSPTFEKALGL
                     AYVPLEMAEPGGPLTLEVRGKPLTARVAETPFYRRPKS"
BASE COUNT         1346 a         3510 c         3408 g         1433 t
ORIGIN      
        1 acgacctcga cccgctccgc cgcttcgagc gctcctggcc cgggctgggc ggcgagctcg
       61 acgccctcct ggagcagccg ccgccgcggg ccgcgtccgg gctcctcgcc ctcgcccgcc
      121 gcgagctcgc cggccgcctc cccggctacc ccgccgccgc catggagacg ctggagcggg
      181 tggtgtcgga ggcaggggaa gcgtgagcga caagccggag atctcggaga gcgaggcggt
      241 catcctctgc gtccaggatg tctggatggc catcggcctc cgctggtccg cgatccgccg
      301 cgagctcgcc gcatttccct tcccgccggg cacgctcgac ggcggccggg ccccgttcga
      361 gctgatgctg gccgccatgg ggctccagat ccagagcgcc gagagcctgc tgagcaccgg
      421 acaggcccgc cgggtgcgcg acctcctgct cacccacttc gtctccgagc acggccgcgg
      481 cgcgctctcc gtcctcgagg cccacgacga cgcctggcgg gaggccctgg caggcggcca
      541 cggcgcggcc ctcggccccg cccaggtgat ggcggagcgg ctcggctgcg ccccgaccga
      601 cgaggccggc ccgagcggcg ccggccgcgc ccccggcagc ccggtcctcc gcgggctcgc
      661 cggggtcctc gagcgctacg gcaccccgga ctggtggcag cgcctcctgg cgagctcgcg
      721 cctggtgccg gacgcggaca tgccggacgc cgacgcttct tcggcgttct gacgccacat
      781 ccttgtggta gcttctcccg ttcacgaacg tgggatgccc gtcatgacca ccgcccctcc
      841 gacctccgcc accgccctcc gctgccgcgg gctggtgaag cgctacgccg acgtcgtggc
      901 cgtcaacggc ctcgacctgg aggtggtgcg cggcgagtgc ttcggcctgc tcggcccgaa
      961 cggcgccggc aagaccacca ccatcgagat cctggagggg ctgacccagc cggacgcggg
     1021 ggaggtcgag gtgctggggc agacctggca gggggagggg caccacctgc gcgagcggct
     1081 cggcgtctcg ctccaggaga cgcagctcaa cgagaagctg accatcatcg agaccctgcg
     1141 cctgttccac tccttctaca ggaacgggcg cgaccccgag caggtgctcg cctccctcgc
     1201 cctggaggag aagcgcgacg cccgcgtcgg caagctctcg ggcggccaga agcagcgcct
     1261 cgccgtcgcc tgcgccctgg tgggcaaccc cgacctgctg ttcctcgacg agccgaccac
     1321 cggcctcgat ccgcagagcc gtctccagct ctgggagcag gtcaccacct ttcagaacgg
     1381 gggcggcacg gtgctgctca ccacccacta catggaggag gcggagcgcc tctgcgaccg
     1441 ggtggcgatc gtcgaccacg gcaaggtgat cgccctcggc acgccggacg agctgatccg
     1501 ctcgctgcac gccgccgacg tcatcgagtt cctctccgag ccgctgctgc cggaggagga
     1561 gctgcgacgt ctccccggcg tctcggacgt ccaccggcgc ggccccaact gggcgctggc
     1621 cgtgagctcg gtcaccgaga gcgtacccgc cctgctcgcc agggtggagg ccgccggggc
     1681 acgcctctct tatctgtcga cccaccgggc cacgctggaa gacctgttcg tgtccctgac
     1741 cggccgggag ctgcgcgatg cctgagcctt cgtccagccg tctgcgcgct ctgcgcgagc
     1801 tgacccgctg ccgcatcctg gagttcacac gcgagccgga agcgatcttc tgggtgttca
     1861 tcttcccggc cctgctcgcc gccgtgctcg gcttcgcctt ccgcgagaag ccgccggacc
     1921 gcatcccggt gggggtggtc gccggcccgg ccgccgaaca gacgcaggcg gcgctggcga
     1981 agtcccccgt gctgctggcg cgggagtacg agacccaggc gggacgggag gcgttgcgca
     2041 ccggcaagat ctcgctgctg gtggagcccg gcccgccggt cgtcttccga ttcgacgaga
     2101 cccgtcccga ctcgcgcatc gcgcggctcg aggcggacga cgccctgcag cgcgccgccg
     2161 gccgggggga cccgctgcag gttcgctcgg agcgggtgac cgagaagggc tcgcgctaca
     2221 tcgacttcct ggtgccgggg ctcctgggga tgaacctgat gggcaccggc atctggagcc
     2281 tggccttctc gatcaccagc gcgcgcaacc gccgcatcct gaagcgtctg gtcgccaccc
     2341 cgatgcggcg gggggactac ctgctgtcgc agatcctggg gcgtctggtc ttcctgatcc
     2401 cggaggtcgt ggtgctggtg gggatcggcg cgctggtctt cggcgtgccg ctgcgcggct
     2461 cgatcctgct gctcggcttc gtctgcctgc tggcggccat gtccttctgc ggcctcgggt
     2521 tgctgatcgc ctccagggtc cgcaccatcg agggggcgag cggcctcgcc aacttcgtga
     2581 tgctgccgat gtggctgctc tcgggcgtct tcttctcctc cgagcgcttc cccgacatga
     2641 tccagccgct ggtccgcgcg ctccccctga ccgcggtcaa cgaggccctg cgcgccatca
     2701 tgaacgaggg ccagggttta gtcggagtcc tcccccagct cgccatcgtc gccgcctggg
     2761 gcctggtgag cttcgtggtg gcgctgaaga tcttcaggtg gcagtagcct gaagattatg
     2821 caggaccacc gtttccagta agggccagat cgtgatggac cgggagttgt tgaaggacac
     2881 cgtgagccga gagagcaggc gtctcgcggc cttaaagcgc gtccaaggtt tcctggaacg
     2941 cccgtctgtt cacctcgcca gtcctcgtcc ttccagggac gagcttcacg agcgctgagt
     3001 ccgcgccatc cgcgcgatcc ggctggcgag gaccgcggcg ccgtagccgt tgtcgatgtt
     3061 caccacgccg atgccggggg cgcaggcgtt gagcatcgtc aggagggcgg cgagacctcc
     3121 gaagctggcg ccgtagccga cgctcgtggg cacggccacc accggcgcgg ggaccaggct
     3181 cgccaccacc gtcgggagcg cgccgtccat gccggcgacg gcgatcacca cgctggcttt
     3241 ccggatgtcc ggcaggcgcg agagcatccg gtgcaggccg gcgaccccga cgtcgtagtg
     3301 gcggtccacc tcgaagccga accaggcggc gcagacggcg gcctcctcgg cgaccggcag
     3361 gtccgaggtg cccgcggcca gcacggccac cggcccaccc gccttctcgt gcggctcgcc
     3421 gcaggtcaga acccgcgccc gctcatggta gaccgcgctc gggaaaactc cctgcagggc
     3481 atccgccgtc tcctgccgga tgcgggtcac cagcacccgg ccgttggctt ccatcatgcg
     3541 ctcgacgatg acccgcaggt cgtccgtccg cttgccctcg ccgtagacgg tctcgggcag
     3601 gccgttgcgc agctcgcggt ggaggtccag cttggcgaag ccgaggtcgg cataggggag
     3661 ctgtgccaga tcctcgaggg ccgcctcggg ggtgagggcc ccggcggcga cggcgtccag
     3721 gatctccttg atctgatggg gattcatggc gtcaccggca gatcggcgcc gccccggcgg
     3781 taaccgtggg ggtcgtaggc caccctttcg aagcccaggg agcgaaggcg cggctccacg
     3841 tcgttccaga tcccggcgag gcgttcgacc tcgtccgggg cgacctccac ccgcgccgct
     3901 ccctggtcgt gccgcacgcg gaccacccgg aagccgtgct ccgcgagcag ctcctcggcg
     3961 gcctcgatgc gccgcagctt gcccagggtg acgagctgcc catgcgggat gcgcgacgag
     4021 aggcaggcgt tggccggccg gtcccagacc gagaggccca gcgcgctcgc gatggcgcgg
     4081 gtcgccgcct tgtcgatccc gtgctccagg aggggtgagt agacgccgct ctcgcgcacc
     4141 gccttcaacc cgggccggtc gggtcccggg tccgacgcgt tggtgccgtc gcagaccacg
     4201 gcatagccgc gctcggcggc caggcggacc atcgtgctca tccgcagccc ctggcagacg
     4261 tagcagcggt gcgacgggtt ggctacgaac tcgggatcgt ccagctcgct gtaggtgacg
     4321 gtctcgtgcc ggatgccgat ctccgcggcc aagcgccggg cgtgatccag ctcggccccg
     4381 gccagtgtct ccgccgccgc cgtgaccgcc accgcccgct cccccaccgc acggaacgcc
     4441 agcgccgcca ccaccgccga atccactccg cccgaatagg cgaccagcac cgagccccgg
     4501 ccggcgaggt cggtgacaac gtcggcaatg acggtgtcga tgcggagaga gagatccata
     4561 ctgctcatcg cgggcgatgg taccaccggc ttcttacagc tttcacttcg gcagccggcg
     4621 cagcgggaac ttgatcggca cccagaagaa gatggcgaag acgccgaagg cgtagagggt
     4681 cacgagggga gcggcggcct gcatctcgaa gggggccatg gcgcggagga gatagatcag
     4741 gcccggcggg ccgacgaagt agaggaggaa gacgcggcgg cgcagggggt cggggaagtc
     4801 cgccctgcgc cgctcgtaga agtagccgct gatccagccc agagacatgg cggcgctgta
     4861 cagcgggtag aaccccagcg ccaggctccc ggccaccgtg accatgcgga ggaagaccaa
     4921 caggtccacc acccagcacg ccaggaacag gccgaagagc accgtctcga gccgtgtcac
     4981 cgggccgcgc cgacctccag tgcctccgcg tccaccggtt cggcatagtc ctccatgggc
     5041 ggacaggtgc agaagaggtt gcggtcgccc caggcgtcgt cgacccgccc gacgtacggc
     5101 cagaacttgc gttcccgcac ccaggcggcc gggaaggcgg cctgctcccg gctgtagggc
     5161 cggtcccagc cgctctccgt cacgtcctcc agggtgtgcg gcgcgtactt gagcgggttc
     5221 tccttgcggt cggcccgccc ctcttcgatg gcgcggatct cgtcgcggat ggcgatcagg
     5281 ccgtcgcaga gacggtccag ctccgccctc gactcgctct ccgtcggctc gatcatcagc
     5341 gtcccggcca cggggaacga catcgtcggc gcgtggaagc cgtagtccat cagccgcttg
     5401 gccacgtcca cggcctcgac gccggcgctc gccttgaacg gccgcaggtc gaggatgaac
     5461 tcgtgcgcca cccggccgcg ccggccgctg tagagcacct tgaagtacgg cttcaggcgc
     5521 tcggccatgt agttggcgtt gaggatcgcc acctcgctgg cgcgggtgag cccctgggcg
     5581 cccatcatcg cgatgtaggc ccaggagatg ggcaggatcg ccgcgctccc ccacggggcc
     5641 gccgacaccg ctcccatccc ctcgcccccg ccgacgtgga ccaccggatg ccccggcagg
     5701 aacggcgcca ggtgctcggc gacgcagatc ggccccatgc cgggaccgcc gccgccatgc
     5761 gggatgcaga aggtcttgtg caggttgagg tggcagacgt cggcgccgta gtcgcctggg
     5821 cggcagaggc ccacctgggc gttcatgttg gcgccgtcca ggtagaccag cccgccgtgc
     5881 cggtggacga tgtcgcagat ctcccggatc tcctcctcga acacgccatg ggtcgacgga
     5941 tacgtgacca tcagcgccgc cagccggccg gcgtgcgcct cggccttggc ccgcaggtcg
     6001 tccagctcga cgttgcccga ggcgtcgcag gccaccggca ccaccttgag ccccgccatc
     6061 accgccgacg ccgggttggt gccgtgcgcc gacaccggga tcaggcagac gtcgcgctcc
     6121 ccctggcccc gctcgcgccc ctcttgacga tggtacgccc ggatgaccat gaggcccgcg
     6181 tactcgccct gcgagccggc gttcggctgc agggagcagg cggcgaagcc ggtgatctcg
     6241 accagccact gctccagggt gcggaacagc tcctggtagc cgcgcgtctg ctccaccggc
     6301 gcgaacgggt ggatccccgc gaagccggcc caggtgatcg ggatcatctc ggtggtggcg
     6361 ttcagcttca tcgtgcacga gccgagcggg atcatcgacg tggtgagcga caggtcccgc
     6421 ccctcgagac gcttgatgta gcgcagcatc tcgtgctcgg tgtggtagcg gtggaagacc
     6481 gggtgggtca ggtagggcga ggtccgcgcg tgcggcgccg ggatgtcgga gtcgatacca
     6541 gaggccagct ccgcagcctt gaagcccggg gcctgatcgc ctccgaagac cgcgaacagc
     6601 gcgtccaggt cggcggccgt ggccgcctcg tcgagggcga tgccgacggt gctctccccc
     6661 agcggccgga ggttgatgcg gcgcgccgcg gcggcggcga tgatctcgga ggcggacctc
     6721 cccttcacct ccacccgcag ggtgtcgaag aacggctcgc ggccggcctc gtggccgagc
     6781 cggcgcagcc cctcggcgag gacggcggcc agggcgtgca cccggcgggc gatgcgggcg
     6841 agcccctcgg ggccgtgata gacggcatag aagccggcca tcaccgccag cagcacctgc
     6901 gccgtgcaga tgttgctcgt cgccttctcg cggcggatgt gctgctcgcg ggtctgcatc
     6961 gccatgcgca gggcggtgtg cccccgccgg tcgcgcgaga cgccgatgat ccggcccgga
     7021 agctggcgct tgtactgctc gctggtggcg aggaaggcgg cgtgcgggcc gccgtagccc
     7081 agggggacgc cgaagcgctg ggcggagccg accgcgatgt cggcgccgat ctcgccgggc
     7141 ggccgcagca gcgccagcga cagcaggtcc gccgccatca ccaccagcac cttggcctcg
     7201 tgggcgcggc gcaccagcgg ctcgtagtcg accacccggc cgtccgtggc cgggtactgg
     7261 aggaggatgg cgaagacgcc gtccaggtcc ggcgcctcgg ggcgcccgac gcgcacctcg
     7321 acgccgatcg cctcggcgcg ggtgcggacc acggcgaggg tctgcgggtg gcagtcctcg
     7381 gcgacccaga aggcgggccg gtcctcgtcg gtcagggcgc ggcacatgtg catggcctcg
     7441 gccgcggcgg tggcctcgtc gagcagcgac gcgttggcca ccggcaggcc ggtcaggtcg
     7501 gccaccatgg tctggaagtt gagcagcgcc tccaggcgcc cctgggagat ctcggcctgg
     7561 tacggggtgt actgggtgta ccagcccggg ttctccagga cgttgcgctg gatgaccgtc
     7621 ggcaccaggc agtcgtggta ccccatgccg aggaaggagc ggaacacctg gttgctgtcg
     7681 gcgagccgcc gcagggcgtc cagggtctcg cgctcaccca gcgtgcggtc ctcggggagc
     7741 tcggccagac gcaggccgcc gcgcagccgg atggactccg gcaccgtctc gcgcaccagg
     7801 ccctcgaacg agtcgagccc cagggacgcg agcatctccc gggcctcgga ctcgttcggg
     7861 ccgatgtgcc ggcggacgaa ggtgtcgacc ggagcgagcg gatcgagaat ggaaggctca
     7921 ggcttcgcca ctcttgacgt actcctcgta ctcttcggct ttcatcagcg acttcaggtc
     7981 cgccgcggac gagaaccgga tcttgatcaa ccaaccttcg ctctggggat cctcgttcag
     8041 cagctcggga tcgtccacca cggcgtcgtt gacctcgacg atctcgccgg ccaccggggt
     8101 gtagacctcg gccacggcct tcaccgactc gatggtcccg atctcgtcgc cgctgttgaa
     8161 gacctgtccg gcctccggca gctcgacgaa gaccacctct cccagctcct gctgcgcgaa
     8221 ctccgtgatg cccacggtgc acacgtcgtc gtcaacgcgg acccactcgt gctccctcga
     8281 atagagatat tcgctcggat acatacgctc tctgcaccct ccagatttgg gctggtccga
     8341 ccggggccga ccgaagcgtc cgcacgaagg ccggacgaga gatgtcgctg cggtgggggg
     8401 ccgggagttc tcttccttat tggatggccg tcgctccagt tataccacca aacacccccc
     8461 cggctctccg gagggcacgg caagggctca ggatgccccc tcaggacttg ggccggcgat
     8521 agaacggggt ctcggcgacc ctggcggtga gcggcttgcc ccgcacctcc agggtcaggg
     8581 gcccgccggg ctccgccatc tcgaggggga cgtacgccaa tcccaacgcc ttctcgaacg
     8641 tggggctcca ggtgccgctg gtgacgtcgc ctaccttctc tccccccgcg gaggagagaa
     8701 ccccgtgccc ctggcgggcg attccccgcc cgtcgacctc gaagccgacc agcttgcggc
     8761 gcagcccgtc gtgccgctgg gcggcgaggg cgtcgcggcc gaggaactcc cccttgtcca
     8821 gcttgaccac ccagccgagg cccgcctccc agggggtcgt ggtctcgtcg atctcgtggc
     8881 cgtagagcgc catggccgcc tcgagccgca gggtgtcgcg ggcgccgaga ccggccggga
     8941 ccgctccggc ggccacgagc cggcgccaga tctccggcgc gtcgtccggg tcgacgtaga
     9001 gctcgaagcc gtcctcgccg gtgtagccgg tgcgcgagat cagcgccggc cgcccggcca
     9061 cctccccctg gaggaagccg tagtagcgcg tcgcggcggc gccgggggtg gcgagcgggg
     9121 tcaggatctc caccgccttc ggcccctgga gggcgaggag ggcgtagcgg tcgctctcgt
     9181 cgatcacctc gacgtcctcg gcgctgtcca ctcgcgaggc gatccactcg aagtcgcggg
     9241 cggcgttgga ggcgttgacc accaccatgt agtcgccgtc ggccagcttg tagaccagga
     9301 cgtcgtcgat gtaggtgccc tgctcggtga gcaggccgct gtagtgggcg cggccgggca
     9361 cgagcttgga gacgtcgttg ggggtgaggc gctggagcag cgcctcggcg cccgctcccc
     9421 gcacccggac ctcccccatg tgggagacgt cgaacaggcc ggcggcggtg cgcacggcgc
     9481 ggtgctcgtc gatgactccg gcgtactgca ccggcatctc ccagccggcg aacggcacca
     9541 gccgggcgcc ctgctccacg tggcactcgt ggagcggcgt ccgccggagg ctctcggtgc
     9601 tctcgctgct ctcggacatg tctgacggtt ctcctggctg atctctggat tgcgggccgg
     9661 cggggtcccg cccggcgcag cgggaacgct atcacag
//
LOCUS       DASPJI010000284         3661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_761568, whole genome shotgun sequence.
ACCESSION   DASPJI010000284 DASPJI010000000
VERSION     DASPJI010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761568"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VEL74_07555"
     CDS             complement(<1..432)
                     /locus_tag="VEL74_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62421.1"
                     /translation="MDAAAYERFTERLLQSLAADSTVLGLVALGSMAALDTKPDRWSD
                     HDFFVIVRTGEQEGFRRDLSWLPDRGNLVLSYRETAHGLKALYEDGHLVEFAVFDPGE
                     LHLAKVNRYRVLLDRGGEVTAAMARIREESERWARTSGLGDF"
     gene            complement(439..1314)
                     /locus_tag="VEL74_07560"
     CDS             complement(439..1314)
                     /locus_tag="VEL74_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62422.1"
                     /translation="MDTMSPTGRPMMSEAPSREVPPRETLVERTRELLVRDGLPRLQM
                     SGILVLTGAAGFFTSFLLLEFGLDSMAVRYPTAVLAAYGVFLLLLRLWLRLQERGWEN
                     VAEALDLTQAVVDGAELVGRGVRGAGDFVGGGGRFGGGGASASFEAPTSPERVPLRAS
                     VPRGGGSKGGSSSGFSLGLDLDEGGIFFLVAILLMAVAALGAAFWVVWSAPVLLAEVL
                     VDGLIMAGLYRRLRKPQPGHWLAGAVRRTCVPALVVALLFFFAGWLLQRAAPDARSIG
                     GAWKTIQADQTDGPR"
     gene            1470..1946
                     /locus_tag="VEL74_07565"
     CDS             1470..1946
                     /locus_tag="VEL74_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYG62423.1"
                     /translation="MSETSYKLDELATAAGVSPRTVRYYVQRGLLPAPAFRGRDTAYS
                     GEHLLRLRAIKRLQERFLPLDAIQAELEKSSPAELEILASEGGGPWPPPVPSRGHLRF
                     PGPPPPPRMPLPLPRAGEKVVRYVLRPGLELHLSERADAATRALCEKILNEAEAER"
     gene            1943..>3661
                     /locus_tag="VEL74_07570"
     CDS             1943..>3661
                     /locus_tag="VEL74_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HYG62424.1"
                     /translation="MTNAGLYSKDGGPIPLRGVEVTGEVLGGYAQVTVRQRYQNAEAK
                     PVEAVYTFPLPSDATLSGFAMTCAGRRLDGVVKEREAAFRDYDDALFAGHGAALLEQE
                     RTNVFTASVGNLLPGEETIVEVTYLQRVPADEGELRWSVPTLVAPRYIPGNPAGDRTA
                     HGSADPTDQVPDADRITPPLGQVGYGLTLDLVFDLGVSVEVESPSHDIDAQTEGNRTR
                     VRFRQGEVVLDRDVVLIARGHSGGGDSRIESVIAHRPATGPGYLALTVIPDLAGDGAK
                     VPPQDVVFVIDTSGSMSGASLPQAQAALRLCLRHLREGDRFNVIEFNSDWKGFASAPV
                     PFTQRALEEADRWVEALEATGGTEMMEPLTAAVKMAPDGVVVLLTDGQVGNEAAILKA
                     LLATRRSGRVYSFGIGTNVSDSLLGDLARKTGGAVELIHPGERIDDKVVAQFARAIAP
                     RISDIKVAFKGVEVEDLAPEEPPALIDGEPWVMLGRIENGERGRAIVTGNLDGRPFRL
                     EVPIDLAGAGERPAVAKLWAQERVRALTDAMHSVDGRRAGRLKERIVALALEHGLSTP
                     YTAFLVI"
BASE COUNT          559 a         1269 c         1337 g          496 t
ORIGIN      
        1 gaagtccccc agtccggagg tccgcgccca ccgctccgac tcctcccgga tgcgcgccat
       61 ggccgcggtg acctcgccgc cgcggtccag cagcacccgg tagcggttca ccttcgcgag
      121 gtggagctcg ccggggtcga agacggcgaa ttccaccagg tggccgtcct catagagagc
      181 cttgagcccg tgggcggtct cgcggtagga gaggaccagg ttcccgcgat cggggagcca
      241 ggagaggtcc cgccggaaac cctcctgctc gccggtccgg acgatgacga agaagtcgtg
      301 gtccgaccag cggtccggct tcgtgtcgag ggccgccatg gagccgagcg ccaccaggcc
      361 cagcaccgtg ctgtccgccg ccagactctg gagcagacgc tcggtgaagc gctcgtaggc
      421 cgcggcatcc atgggagatc agcgcgggcc gtcggtctga tcggcctgga tcgtcttcca
      481 ggcgccgccg atggagcggg cgtccggcgc ggcgcgctgc aggagccagc cggcgaagaa
      541 gaagaggagg gcgaccacca gggcggggac gcaggtgcgg cgcaccgcgc cggcgagcca
      601 gtggccgggc tggggtttcc tgaggcgccg gtagaggccg gccatgatga ggccgtccac
      661 cagcacctcg gcgaggagga ccggcgcgct ccacaccacc cagaaggccg ctccaagcgc
      721 cgccacggcc atgaggagga tggcgaccag gaagaagatc ccgccttcgt cgaggtccag
      781 gccgaggctg aagccgctgg aggagccccc cttcgagccg ccccctcggg gcacggaggc
      841 ccgcaggggg acccgctcgg gggatgtcgg cgcctcgaag ctcgcggagg cgccgccacc
      901 gccgaaccgg ccgccgccgc ccacgaagtc gccggcgcca cggacccctc gtccgacgag
      961 ctcggcgccg tccacgaccg cttgagtcag gtccagggcc tccgccacgt tctcccagcc
     1021 cctctcctgg agccgcagcc agaggcgcag cagcaggagg aagacgccgt aggcggcgag
     1081 gacggcggtc gggtagcgga ccgccatcga gtccaggccg aattccagga gcaggaagga
     1141 ggtgaagaag ccggccgcgc cggtgagcac caggatgccc gacatctgaa gacgcgggag
     1201 gccgtcgcgc acgaggagct cgcgggtcct ctcgacgagc gtctcgcgcg gcgggacctc
     1261 ccgggagggg gcctcgctca tcatgggccg gccggtgggt gacatggtgt ccattctatc
     1321 ctcctggatc cgcccgccgg agccaccggc gggccgcctc tacccgagag tgccggatcc
     1381 gctccctcca tttcgtccat ctccctggcg atctcgacag ctcatcttgc ctacttgaca
     1441 gtatcgctgt ctagatttat cctcgtccca tgagtgagac gagctacaag ctggacgagt
     1501 tggcgacggc ggcgggggtg tcgccgcgca cggtgcgcta ctacgtgcag cggggcctgt
     1561 tgccggcgcc ggccttcagg ggacgggaca cggcctactc gggcgagcac ctgctccggc
     1621 tgcgggcgat caagcggctg caggagcgtt tcctgccgct cgacgcgatc caggcggagc
     1681 tggagaagag ctcgccggcg gagctggaga tcctggcctc tgagggcggg gggccctggc
     1741 cgccgcccgt gccctcgcgc gggcacctgc gtttccccgg gcctccccct cccccccgca
     1801 tgcccctgcc gctccctcgc gccggcgaga aagtcgtgcg ctatgtgctg cggccggggc
     1861 tggagctgca cctgtccgag agggccgacg cggcgacgcg cgccctgtgc gaaaaaatcc
     1921 tgaacgaggc ggaggccgaa cgatgacgaa cgcggggctg tactcgaagg acggcggacc
     1981 gatccccctg cgcggggtgg aggtgacggg cgaggtgctg ggggggtatg cccaggtgac
     2041 ggtgcgccag cgctaccaga acgccgaggc gaagccggtg gaggcggtgt acaccttccc
     2101 gctgccgagc gacgcgacgc tgtccggatt cgccatgact tgcgccggcc gccgcctcga
     2161 cggcgtggtc aaggagcggg aggcggcctt ccgggactac gacgacgcgc tgttcgccgg
     2221 acacggcgcg gcgctcctgg agcaggagag gaccaatgtc ttcaccgcca gcgtcggcaa
     2281 cctgctgccg ggagaggaga cgatcgtcga ggtcacctac ctgcagcgcg tcccggccga
     2341 cgagggagag ctgcgctggt cggtcccgac cctggtggcg ccgcgctaca tcccgggcaa
     2401 cccggcgggc gaccgcaccg cccacggctc ggcggaccca acggaccagg tccccgatgc
     2461 cgaccgcatc acgccgcccc tgggccaggt gggctacggc ctcaccctgg acctcgtctt
     2521 cgacctgggc gtctccgtgg aggtggagag cccgtcgcac gacatcgacg ctcaaaccga
     2581 gggcaaccgg acccgggtgc gctttcggca gggggaggtg gtgttggacc gcgacgtggt
     2641 gctgatcgct cgcggccact ccggcggggg agactcccgc atcgagtcgg tgatcgccca
     2701 tcggcccgcc accggccccg gctatctcgc cctcaccgtg ataccggacc tcgccggcga
     2761 cggagcgaag gtaccgccgc aggatgtggt gttcgtgatc gacacctcgg gctcgatgag
     2821 cggcgcgtcg ctcccccagg cccaggcggc gctccgcctc tgcctgcgtc acctgcggga
     2881 gggggaccgc ttcaatgtca tcgagttcaa ctccgattgg aagggtttcg cgagcgcgcc
     2941 cgtgccgttc acccagcgcg cgctcgagga ggcggaccgc tgggtcgagg cgctcgaggc
     3001 caccggcggg accgagatga tggagccgct taccgcagcg gtgaagatgg ctcccgatgg
     3061 cgtcgtcgtg ctgctcaccg acggccaggt gggcaacgaa gccgccatcc tgaaagcgtt
     3121 gctcgctacc cggcgcagcg gccgcgtcta ctccttcggg atcggcacga atgtgagcga
     3181 tagcttgctg ggcgacctcg cccggaagac gggcggcgcg gtggagctca tccatcccgg
     3241 cgagcggatc gacgacaagg tggtggccca gttcgcccgc gccatcgcac cgcgcatcag
     3301 cgacataaag gttgctttca aaggcgtcga ggtggaggat ctggcgcccg aggagccgcc
     3361 ggcgctgatc gacggcgagc cgtgggtgat gctcggcagg atcgaaaacg gcgagcgcgg
     3421 gcgcgcgatc gtcaccggca accttgatgg ccggccgttc cggctcgagg tgccgatcga
     3481 cctcgccgga gcgggggagc ggccggcggt ggcgaagctc tgggcccagg agcgcgtgcg
     3541 cgctctcacc gatgccatgc actcggtcga cggccggcgg gcgggccggc tcaaggagcg
     3601 gatcgtcgcg ctcgcgttgg agcacggcct ctcgacgccc tacaccgcat tcctggtgat
     3661 c
//
LOCUS       DASPJI010000285         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_761715, whole genome shotgun sequence.
ACCESSION   DASPJI010000285 DASPJI010000000
VERSION     DASPJI010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761715"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VEL74_07575"
     CDS             <1..1152
                     /locus_tag="VEL74_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62425.1"
                     /translation="GPYQITGTVENRVGNLVRTELTVQAGTHPLDRFKMVRLVKDGPA
                     RQLVGSILLLPPLGPGFTFYEQRDESGGVGTSIAEYFALRNFDVYGYSPRMEGIPAGT
                     CEAGVLDCSAMAGWNLASMVEDIAFVRSRIEALHPGTRVVAGGASLGGMLAIALANSA
                     PQDYDGIFPWEGMLYSQDPQVRALNQGYCAALEAQLAAGAVFDGVGINVFKAVVQYAK
                     TAPGGLTPIPLFPPTLTNHQVMVLLLSTSSPGPITMPVPNYIQMNGSLAQDRLFFASE
                     PRLIENISRFFNYAPVAVTRDISCSLAGSETGYTSNLGAYTGSVLMIGGGRAFGAYMD
                     DQLARFTGTSDKRLLLEPEFGHIDHFMTPRHRQFVERPIYEWALRVLGR"
     gene            complement(1174..>2616)
                     /locus_tag="VEL74_07580"
     CDS             complement(1174..>2616)
                     /locus_tag="VEL74_07580"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYG62426.1"
                     /translation="RVDLGEGLQAQVRVSEIVPFPGWSGLHADVELPAGQTPEAALQA
                     AVAEARRPAPTGSPQRAQAPALPDAVFRPDAAYAGMAEPDLTHRQLAVIRAWNVIHFF
                     YPYLHLIGDWDAVLPEYLARMETAGTGRDYALTVAEMMARVADGHTRVVHPEVNRYYG
                     EVGLPLQVRWIEGAPVVTAVRDDAHRLGIEVGDALLAVDGEPVAAAIERLGRYVAAST
                     RPALLARICPMLLRGPDGSTATLSLQGLDGTTREVKLKRDRRTYYYVPPPAGDTVRIL
                     PGNLGYADLTRLTPPEVDGMFERLRDTRAIIFDMRGYPNGTAWPIAPRIHTRDTRNAA
                     TFRRALVSAFNEEEAEGAFAFSQPVPKDPRGLPRYTRPTVMLIDERAISQSEHSGLLF
                     EAANGTRFIGTATAGANGDVTNFTLPGGITVAFTGHDVRHADGRQLQRVGLTPHVEAA
                     PTRKGLREGKDEVLERAVRFLEEELGGEGR"
BASE COUNT          357 a          939 c          934 g          386 t
ORIGIN      
        1 ggtccttacc agatcacagg cacggtggag aaccgggtcg gcaacctggt ccgcaccgag
       61 ctcaccgtcc aggcggggac ccatcccctg gaccgtttca agatggtccg cctggtcaag
      121 gacgggccgg cgcgccagct cgtgggctcc atcctcctgc tgccgcccct gggcccgggc
      181 ttcaccttct atgagcagcg cgacgagagc ggcggcgtgg gcacctcgat cgccgagtac
      241 ttcgcgttgc ggaacttcga cgtctacggc tactcgccgc gcatggaggg cattccggcg
      301 gggacctgcg aggccggcgt cctcgactgc tcggcgatgg cgggttggaa cctggcctcg
      361 atggtggagg acatcgcctt cgtccgctcg cgcatcgagg cgctccaccc aggtacccgt
      421 gtcgtcgcgg gcggcgcctc gctgggaggc atgctcgcga tcgcgctcgc caactccgcc
      481 ccccaggact acgacggcat cttcccctgg gaggggatgc tgtacagcca ggacccgcag
      541 gtgcgggcgc tcaaccaggg ctattgcgcc gcgctggagg cccagctcgc ggccggcgca
      601 gtcttcgacg gcgtgggcat caacgtcttc aaggcggtgg tccagtacgc caagacggcc
      661 cccggcggcc tgactccgat cccgctcttc ccgccgacgt tgacgaacca ccaggtgatg
      721 gtcctcctgc tgagcacctc gtcgcccggc ccgatcacca tgccggtgcc caattacatc
      781 cagatgaacg gcagcctggc ccaggaccgg ctcttcttcg cctccgagcc gcggctgatc
      841 gagaacatct cgcggttctt caactacgcc ccagtggccg tgacccgcga catctcctgc
      901 tcgctggccg gctccgagac cggctacacc tcgaatctcg gggcctacac cggttcggtg
      961 ctgatgatcg gcggcggccg cgccttcggc gcctacatgg atgaccagct cgcccgcttc
     1021 accggcacca gcgacaagcg cctcctcctg gagccggagt tcggccacat cgaccacttc
     1081 atgaccccgc gccaccggca gttcgtcgag cgacccatct acgagtgggc cctccgcgtg
     1141 ctcggccgct gagcccgggc ccggggcgga gggtcagcgc ccttcgcccc ccagctcctc
     1201 ctccaggaac cgcaccgccc gctccagcac ctcgtccttc ccctcgcgca gccccttgcg
     1261 ggtgggggcg gcctccacgt gcggagtgag gccgacgcgc tggagctgcc ggccgtcggc
     1321 gtggcggacg tcgtggccgg tgaaggccac ggtgatgccc cccggcaagg tgaagttggt
     1381 gacgtcgcca ttggcgccgg cggtggcggt gccgatgaag cgggtgccgt tggcggcctc
     1441 gaagagcagg ccggagtgct cggactggct gatcgcccgc tcgtcgatca gcatcacggt
     1501 agggcgcgtg taccggggca gcccccgggg atccttcggc accggctgcg agaaggcgaa
     1561 ggcgccctcg gcctcctcct cgttgaaggc cgagaccagg gcgcggcgga aggtggcggc
     1621 gttccgggtg tcccgggtat ggatgcgcgg cgcgatcggc caagcggtgc cgttggggta
     1681 gccgcgcatg tcgaagatga tcgcccgggt gtcccggagg cgctcgaaca tgccgtcgac
     1741 ctccggcggc gtgagccgcg tcaggtccgc gtagccgagg ttgccgggga ggatacggac
     1801 ggtgtcgccg gccgggggag ggacgtagta ataggtccga cggtcgcgct tcagcttcac
     1861 ctcgcgggtg gtgccgtcga gcccctggag cgacagggtg gcggtggagc cgtccgggcc
     1921 gcgcagcagc atcgggcaga tccgtgccag cagcgcgggg cgggtggacg cggcgacgta
     1981 gcggccgagc cgctcgatgg ccgccgccac cggctcgccg tccaccgcga gcagggcgtc
     2041 gcccacctcg atccccaacc ggtgggcgtc gtcgcggacc gccgtcacca ccggcgcccc
     2101 ctcgatccag cgcacctgga gaggcaggcc gacctcgccg tagtagcggt tcacctcggg
     2161 gtgcacgacg cgggtatggc cgtcggcgac gcgcgccatc atctcggcga ccgtcagcgc
     2221 gtagtcccgg cccgtgccgg ccgtctccat gcgggcgagg tactcgggga gcacagcgtc
     2281 ccagtcgccg atcaggtgga gatagggata gaagaagtgg atcacgttcc aggcgcggat
     2341 caccgcgagc tggcggtgcg tcaggtccgg ctccgccatc ccggcatagg ccgcgtcggg
     2401 acggaacacg gcgtccggca gcgccggcgc ctgggcgcgc tggggggagc cggtgggggc
     2461 cggccggcgc gcctccgcca cggccgcctg gagcgccgcc tccggggtct gtccggccgg
     2521 cagctcgacg tcggcgtgga gcccggacca ccctgggaac ggcacgatct cgctgacccg
     2581 cacctgggct tggagccctt cgccgaggtc gacccg
//
LOCUS       DASPJI010000286         4694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_761766, whole genome shotgun sequence.
ACCESSION   DASPJI010000286 DASPJI010000000
VERSION     DASPJI010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761766"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VEL74_07585"
     CDS             complement(<1..762)
                     /locus_tag="VEL74_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62427.1"
                     /translation="MSKLTVHRGMATWGERLVPLGGFREATARAGLRLSPEERAAALE
                     AYSAPVPDPTPVEGPYSRIAVFGGIYNNHYGLATLLEDAARRGAEAVYCLGDLGGFGP
                     NPEKVWPLLLQGGVRSIQGNYEESLASGREDCNCGYTDPRDNHFAAISYQSTERGCSP
                     AFKAWMGSLPRRRRVRVGERELLLVHGSPRRINEFLFRSTSPVPFLEVLLDQQRCDGL
                     LCTHTGLHWHRRLPSGRDVVNAGVIGRPANDGDTRV"
     gene            complement(774..2807)
                     /locus_tag="VEL74_07590"
     CDS             complement(774..2807)
                     /locus_tag="VEL74_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYG62428.1"
                     /translation="MALMAVERAVRERYRQGAQERVGDLCCPVSYDPRYLEVIPKEVL
                     ERDYGCGDPSRHLKEGETVLDLGSGTGKICFIAAQVVGPRGRVIGVDMTDEMLQVARD
                     SAPVVAERLGYANVEFRRGRIQDLALDIDLLDETLAREPVTSTEALLRAAARADELRR
                     SRPLIAGDSIDVVVSNCVLNLVAGDEKPRLFQEIYRVLRSGGRAVISDIACDEPVPAE
                     LRQDPELWSGCIAGAMTESGFLEAFEEAGFHGIRILERQREPWRTVAGIEFRSVTVEA
                     FKGKQGPCLERRQAVIYKGPFREVLDDDGHRMRRGERYAVCGKTFRLYQREPYGSCFE
                     LIEPLEEVPLAAAQPFDCSRTALRHPRETKGRDYEATTEAKPCWSRGAVAEGAAMNAS
                     GVPKVSLRSLDTLWFQVGGTLCNLACTHCFVSCSPTNHTHEFIGLAEVERYLEEAVAL
                     GVREYYFTGGEPFLNPEMEAILRATLAAGPASVLTNGLLLDPDRCGRLAAMAGESDYS
                     LDLRISLDGYDAATNDPIRGDGTFERILAGVRNLAAAGIDPVITVTEVCAEAATASGK
                     ERFLEMLREMGLAHPRLKVLPVFRIGAEAERGGAYESWQRLRGDEVPEGAWDHLQCST
                     CRMVTDQGVWVCPILVNEPSGKMGETLADTLGSFPLEHPACWTCHVFGVSCRT"
     gene            2950..3360
                     /locus_tag="VEL74_07595"
     CDS             2950..3360
                     /locus_tag="VEL74_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62429.1"
                     /translation="MRRFLLFALPLLVLAMALFHFALEAFGLDPDPALLSRSGAPVLP
                     AWVTLGTWCLEAVGLAALFLLIQGRGGWRWSTGLLAGWIAWVFRGPLLVLAAVGLGGL
                     PPGPWWWLALSWWILYSLCGLLMGLLAAAAGLQP"
     gene            3484..4539
                     /locus_tag="VEL74_07600"
     CDS             3484..4539
                     /locus_tag="VEL74_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HYG62430.1"
                     /translation="MRIAAVGRALPPHYYDQQTLLEALRRRWSDRYFNLERLERLHQN
                     VLVGGRHLALPIEEYDGLTTWGRANDAWIRVAQEVGEQAVLDGLARAGLSPADVDALI
                     FVTVTGVATPSIDARLVNRLGLPPRVKRLPIFGLGCVAGAAGIARAADYVRAYPDQVA
                     VLLSVELCSLTLQPEDLSIPNLIASGLFGDGAAAAVVTGGDRPVPSPGPRVVATRSVF
                     YPDSERVMGWDISESGFRIVLSAEVPQVVREFLRRDVDAFLADQGLSRDDISSWVCHP
                     GGPKVLEAMQESLELPAGALDVTWRSLKEVGNLSSTSVLLVLADTLEAHRPAPGSWGM
                     LLAMGPGFCSELVLLQW"
BASE COUNT          606 a         1719 c         1633 g          736 t
ORIGIN      
        1 gacgcgggtg tcgccgtcgt tggccgggcg gccgatcacc ccggcgttga ccacgtccct
       61 ccccgagggg agccggcggt gccagtgaag gccggtgtgg gtgcagagca ggccgtcgca
      121 gcgctgctgg tcgaggagca cctcgaggaa cggcaccggc gaggtggacc ggaacaggaa
      181 ctcgttgatc cgccgcggcg agccgtgcac caggagcagc tcgcgctccc ccacccgcac
      241 gcggcggcgg cgcggcaggc tccccatcca ggccttgaag gcgggggagc agccgcgctc
      301 ggtggattga taggagatcg ccgcgaagtg attgtcgcgc gggtcggtgt agccgcagtt
      361 gcagtcctcg cggcccgacg ccagcgattc ctcgtagttg ccctggatcg agcgcacccc
      421 tccctggagg agcagcggcc agaccttctc ggggttgggg ccgaagcctc cgaggtcgcc
      481 caggcagtag accgcctcgg cgccgcgccg ggcggcgtcc tccaggaggg tcgcgagccc
      541 gtaatgattg ttgtagatgc cgccgaagac ggcgatccgc gagtagggcc cctcgaccgg
      601 cgtcgggtcg ggcaccgggg ccgagtaggc ctcgagggcc gccgctcgct cctcggggga
      661 gagccggagg cctgcccggg ccgtggcctc gcggaagccg ccgagcggga cgagccgctc
      721 tccccaggtg gccatgccgc gatggacggt cagcttcgac acggcgcctc tcctcaggtc
      781 cggcagctca cgccgaagac gtggcaggtc cagcaggcgg ggtgctccag ggggaagctc
      841 cccagggtat cggccagagt ctcgcccatc ttgcccgacg gctcgttgac caggatcggg
      901 cagacccaca ccccctggtc ggtgaccatg cggcaggtgc tgcactgcag gtggtcccag
      961 gctccttcgg gtacctcgtc gccgcgcagg cgctgccagc tctcgtaggc gcccccccgc
     1021 tccgcctcgg cgccgatccg gaagaccggg agcaccttga ggcgcggatg ggcgagcccc
     1081 atctcccgca gcatctccag gaagcgctcc ttgccggagg cggtcgcagc ctcggcgcag
     1141 acctcggtga cggtgatcac cgggtcgatc ccggcggcgg ccaggttgcg gacgccggcg
     1201 aggatgcgct cgaaggtgcc gtcgccgcgg atcggatcgt tggtcgccgc gtcgtagccg
     1261 tcgagggaga tgcgcaggtc cagggagtag tcggactccc cggccatggc ggcgaggcgg
     1321 ccgcagcggt ccgggtcgag gagcaggccg ttggtcagca cgctggccgg cccggcggcg
     1381 agggtggcgc ggaggatcgc ctccatctcc gggttgagga acggctcgcc gccggtgaag
     1441 tagtactccc gcactcccag cgccaccgcc tcctccaggt agcgctccac ctcggcgagg
     1501 ccgatgaatt cgtgcgtgtg gttggtgggc gagcaggaga cgaagcagtg ggtgcaggcc
     1561 aggttacaga gggtcccccc cacctggaac cagagggtgt ccagggaccg cagagagacc
     1621 ttgggaacgc cggacgcgtt catcgcggct ccttcagcaa cagctccccg gctccagcac
     1681 ggcttcgcct cggtggtggc ctcgtagtcc cggcccttgg tctcgcgcgg gtggcgcagt
     1741 gccgtgcgcg agcagtcgaa cggctgcgcc gccgccagcg gcacctcttc cagcggctcg
     1801 atgagctcga aacaggagcc gtagggctct cgctgataca acctgaacgt cttgccacag
     1861 accgcgtagc gctcgccgcg gcgcatgcgg tggccgtcgt cgtccagcac ctcgcggaac
     1921 ggccccttgt agatgaccgc ctggcgcctc tccaggcagg ggccctgctt gcccttgaac
     1981 gcctccacgg tgaccgaccg gaattcaatg ccggcgactg tccgccaggg ctcccgctgc
     2041 cgctcgagga tgcggatgcc gtggaagccg gcctcctcga aggcttccag gaagccgctc
     2101 tcggtcatcg cccccgcgat gcagccgctc cagagctcgg ggtcctgccg cagctcggcc
     2161 ggcaccggct cgtcgcaggc gatgtcggag atcaccgccc tgccaccgct gcggaggacg
     2221 cgatagatct cctggaagag gcgcggcttc tcgtccccgg ccacgaggtt gaggacgcag
     2281 ttggagacca ccacgtcgat cgagtcgccg gcgatgaggg gacggctccg gcgcagctcg
     2341 tcggcgcggg cggcggcgcg cagcagggcc tcggtgctgg tcaccggctc ccgcgccagg
     2401 gtctcgtcga ggaggtcgat gtcgagcgcc aggtcctgga tgcgcccgcg ccggaactcc
     2461 acgttggcgt agcccagcct ctcggccacc accggcgcgc tgtcgcgggc gacctggagc
     2521 atctcgtccg tcatgtcgac cccgatcacc cgtccccggg ggccgaccac ctgggccgcg
     2581 atgaagcaga tcttgcccgt gccgctgccg aggtcgagga ccgtctcccc ttccttcaga
     2641 tgacggctgg gatcaccgca gccgtagtcg cgctcgagga cctccttcgg gatgacctcc
     2701 agatagcgcg ggtcgtagct cacggggcag cacagatcgc ccacccgctc ctgcgctccc
     2761 tgccggtatc gctcccggac cgctctttcc actgccatca acgccatgcg tgctttctcc
     2821 cgttcgcccg tcggctccgc cgctccggac cctcaactat acggcctggg ccccttcgcc
     2881 ggatcgtcga gatggcaggg cgcgcccgcc tctatccgaa atcagccctt cagctatgat
     2941 cgagccgctt tgagacgctt cctcctcttc gccctgcccc tcctcgtcct cgccatggcg
     3001 ctcttccact tcgcgctgga ggcgttcggc ctggacccgg acccggcgct gctgtcgcgc
     3061 agcggcgcgc cggtgctgcc ggcctgggtg acgctcggca cctggtgcct cgaggccgtc
     3121 ggcctcgccg cgctgttcct cctgatccag gggcgcggcg gctggcggtg gagcaccggg
     3181 ctgctcgccg gctggatcgc ctgggtgttc cggggaccgc tgctggtcct cgccgccgtg
     3241 ggcctgggcg gcctcccccc ggggccctgg tggtggctcg ccctctcctg gtggatcctc
     3301 tactccctct gcggcctgct gatgggcctc ctggccgccg ccgccggcct ccagccgtag
     3361 ccatcagcca ccctggcgcc ggtcgagcgc cgaggaatgg ctccgcgagg caggcacgtt
     3421 ctcccgggaa gagtcgccgg acggctgtgc taggcttccc gcgaccgggg gtttcttgcc
     3481 aacttgagaa tcgccgccgt cgggagggcg ctccctcctc actactacga ccagcagacg
     3541 ctgctggagg ccctccggcg gcgctggtcg gaccgctact tcaacctcga gcgtctggaa
     3601 cgcctccacc agaacgtcct ggtgggcggg cggcacttgg cgctgcccat cgaggagtac
     3661 gacggcctca ccacctgggg gcgggcgaac gacgcctgga tccgggtggc gcaggaggtg
     3721 ggtgagcagg cggtcctcga cggcctcgcg cgcgccggcc tctccccggc cgacgtcgac
     3781 gcgctgatct tcgtcaccgt gaccggcgtc gccaccccct cgatcgacgc ccggctggtg
     3841 aaccggctcg ggctgccgcc gcgggtcaag cgcctgccca tcttcggcct cggctgcgtc
     3901 gccggcgccg cggggatcgc ccgcgccgcc gactacgtcc gcgcctatcc ggaccaggtt
     3961 gcggtactgc tgtccgtgga gctctgctcg ctgaccctcc agcccgagga cctctcgatc
     4021 cccaacctca tcgcctcggg actgttcggc gacggcgccg ccgccgcggt ggtcaccggc
     4081 ggcgaccggc cggttccttc gcctggcccg cgcgtggtcg ccacgcgctc ggtgttctat
     4141 cccgactcgg aacgggtcat gggctgggac atctcggagt ccggcttccg catcgtcctc
     4201 tccgccgagg tcccgcaggt cgtccgcgag ttcctgcgcc gggacgtcga tgccttcctc
     4261 gccgaccagg gcctgtcgcg agacgacatc tcctcctggg tctgccaccc cggcggcccc
     4321 aaggtgctgg aggcgatgca ggagagcctc gagctgcccg caggcgcgct cgacgtcacc
     4381 tggcgaagcc tcaaggaggt ggggaacctc tcctccacct cggtcctgct ggtcctggcc
     4441 gacacgctcg aggcgcaccg ccccgcgccg gggagctggg ggatgctcct cgccatgggg
     4501 cccggcttct gctcggagct ggtgctcctc cagtggtaaa taatgaaggg agcgcgatga
     4561 gcactctctt cggcaccgat acgcgcctcc tctataccgg cctcgtgctc ctcgtggccg
     4621 ccgcccggct cgccgagctg cggatcgcca agcgcaacct ggagagcctg ctggcccgcg
     4681 gcggcgtcga ggtc
//
LOCUS       DASPJI010000287         4968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_311478, whole genome shotgun sequence.
ACCESSION   DASPJI010000287 DASPJI010000000
VERSION     DASPJI010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311478"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..495
                     /locus_tag="VEL74_07605"
     CDS             79..495
                     /locus_tag="VEL74_07605"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HYG62431.1"
                     /translation="MQPIKDERSLGQILKELTSETSTLLRQEVELAKTEMSEKATRVG
                     TNVGALAVGGAVAFLGALALLFAVIYGLTSLLDQFMSLRVAVWLAPLLVGLVLAGVGY
                     SMMKKARETLKHEGIAPRKTTDSLQENKEWLKTKMQ"
     gene            471..1859
                     /locus_tag="VEL74_07610"
     CDS             471..1859
                     /locus_tag="VEL74_07610"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HYG62432.1"
                     /translation="MAENKDAVTGTGANSEQIRAEIERTRADMDETFDALEAKLTPGQ
                     LLGEVWSLTKGGSTAGASKLWRVAKEYPMPSAVIGLGLGWLLLESSRGEEHGRTSSRY
                     NRDFDRYGATGRAGYTGTSRFASSYGSGSYTGTTRYESDFDTDYGTDFEGDTGSEGRL
                     SSAAHSAKDKVTGVAHSAKHAVGGAAHNAKEKAGDVADRARYRASELRGQARYKARQA
                     RTGFWQMMESNPLAMGAATLAVGVLAGLAIPSTRKEDELLGETRDHLVEDLKSRGQEA
                     LDKGKQVAEATVDTVKQVAQDEGLTVEGVAEKVRAVGREAKNKVKEEVRNQDLVPGSG
                     NQDQKAGQQNQGQDLGAKPKPEAGEFSGGTSNLGTGSGPSAVGSLGGSNLGGSNLSGG
                     LGGGSNTPSPTVGGVPGESMGSATGPASRGRKTGAAGITGSSGTTGAAGVGGSAREES
                     RAEETEPEFAKR"
     gene            2568..3920
                     /locus_tag="VEL74_07615"
     CDS             2568..3920
                     /locus_tag="VEL74_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62433.1"
                     /translation="MSSHPSTAELQDFLSGGLSARQSRTVVLHLLRGCESCRQIVGPY
                     MQALLGEQPVEAPLSAELETAYDAALDRAFAAACELPLSKSGWSVAVEQALVLFKSRI
                     STGEAETLPSLDEVAAIETLLDLCREVRHENPKRMVQLAQEAVAAAGRLNPDHHGVRR
                     VADLRCRATVELGNAYRVADRLDLAEGLVEQAAAFYLEGTGDPFLGARLFDVQASLDG
                     DRRRFKLACKALDAVHSFHLRHGDQHLAGRALISKGLYTGYSGDCEEAIHLLQSALTL
                     VDAERDPAVAAAGLHNQVSFLVDCGRFREARALLWQNRADLERVSGWVTRLKLRWLEG
                     QINAGLGDLDRAEQALLEVQEGFQKADLHYKAAIASLDLAVLWLRQGRADEARDIVLK
                     AAEVFTALKIHREGLAAVLLLRKAFETRVATTSLLERVIRFMTRAEHDPSVSFKAWFL
                     "
     gene            4094..4501
                     /locus_tag="VEL74_07620"
     CDS             4094..4501
                     /locus_tag="VEL74_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HYG62434.1"
                     /translation="METSYHLGDLQLAIMRVLWSRGESTVADVHDTLEPERGLALTTI
                     ATMLTKMEKKGVVDHRAEGRKFIYRPLVSEGQVRRSMVEDLTSQLFRGDVTALVNHLL
                     SEHEIDRRELAQLRDLISAHEEAHETYESKEER"
     gene            4503..>4968
                     /locus_tag="VEL74_07625"
     CDS             4503..>4968
                     /locus_tag="VEL74_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62435.1"
                     /translation="MFEALMPTWASTAAAWLLTYALHSTLLLGLAWLVSRRLAGRAPL
                     AEEAIWRGALLGAVLTASLQLAGGWEPLAGSWRLESTSPPAAAATRLETAPLPAAARE
                     VAARPLAPRTDEPVAASPAAPSQFPAGAAVLGLWLMGAFLIGAWHARSYLRLH"
BASE COUNT          797 a         1666 c         1690 g          815 t
ORIGIN      
        1 gacggagcgc taccccggca ccgggacggg tgacgcgctc gagaacccgc ggcccttcga
       61 ggccggtgga gtctgatcat gcagcccatc aaggacgaga gatcgctcgg ccagatcctc
      121 aaggagctga cgagcgagac cagcaccctg ctccggcagg aggtcgagct ggccaagacc
      181 gagatgtccg agaaggccac ccgggtgggc accaacgtcg gcgctctcgc cgtcggcggc
      241 gccgtcgcct tcctcggcgc tctggccctg ctgttcgcgg tcatctacgg cctgacttcg
      301 ctcctggacc agttcatgtc gctccgggtg gcggtctggc tcgcaccgct gctggtgggc
      361 ctggtgctcg ccggcgtcgg ctacagcatg atgaagaaag cgcgcgagac cctcaagcat
      421 gaaggcatcg ctcccaggaa aaccaccgac agcttgcagg agaacaaaga atggctgaaa
      481 acaaagatgc agtgacgggt acgggcgcca actccgagca gatccgtgcc gagatcgagc
      541 gcacccgcgc cgacatggac gagaccttcg acgccctgga ggccaagctg acccccggcc
      601 agctcctggg tgaggtctgg agcctcacca agggcggctc gaccgccggc gccagcaagc
      661 tctggcgcgt tgccaaagag tacccgatgc cctccgccgt catcggcctg ggcctcggct
      721 ggctcctcct cgagagctcg cgcggcgagg agcacggccg cacctcctcc cgctacaacc
      781 gcgacttcga ccgctacggc gccaccggcc gggccggcta taccgggacc agccgcttcg
      841 ccagctcgta cggctccggc tcctacaccg ggaccacccg ctacgagagc gacttcgaca
      901 ccgactatgg caccgacttc gagggcgaca ccggctccga ggggcggctc tcctcggcgg
      961 cgcacagcgc caaggacaag gtgaccggcg tcgcccacag cgccaagcac gcggtaggtg
     1021 gcgctgcgca caatgccaag gagaaggcgg gcgacgtggc ggaccgtgcc cggtaccgcg
     1081 cgtccgagct gcgcggccag gcccgctaca aggcgcgcca ggcccgtacc ggcttctggc
     1141 agatgatgga gtccaacccc ctggccatgg gcgcggccac cctggccgtc ggcgtcctgg
     1201 ccggtctggc catcccgtcg acccgcaagg aggacgagct cctgggcgag acccgtgacc
     1261 atctggtcga agacctgaag tcgcggggcc aggaggcctt ggacaagggc aagcaggtgg
     1321 ccgaggccac ggtcgatacg gtcaagcagg tggcgcagga cgaggggctg accgtggaag
     1381 gcgtggccga gaaggtccgg gccgtgggcc gcgaggccaa gaacaaggtc aaggaggagg
     1441 tccgcaacca ggatctcgtc ccgggctccg gcaatcagga tcagaaggct ggccagcaga
     1501 accaggggca ggacctggga gctaagccga agccggaggc cggcgagttc tcgggcggca
     1561 ccagcaacct ggggaccggc agtggcccga gcgccgtcgg cagcctgggc ggcagcaacc
     1621 tgggtggcag caacctgagc ggcggtctgg gcggcggcag caacacgcct tcgcccaccg
     1681 tgggcggcgt gccgggcgag agcatgggga gtgccaccgg tccggcgagc cgcggccgta
     1741 agaccggagc cgccggcatc accggaagct ccggaactac cggagcggcg ggggtcggcg
     1801 gctcggcgcg ggaggagtcc cgggccgagg agaccgagcc ggagttcgcc aagcggtaac
     1861 cctgccgcac ccatcccagt accctccggc cccgggagtc ttcccggggc cgggcttttt
     1921 ccccttcccg ctttccttca gggcgaacca ggtgtaaatt agtggtgcct tgccgggccg
     1981 ccccgccttt gggtcggatg gtggatcgct tttgggctcg gccggcacta tatctttgtt
     2041 gaaggtccct tgtagccaca atcccgtcat gagccctgac ccctccgagg gtcacggtag
     2101 ctctgcgagg aagaggaggt agagaatgtc cggccggatc cttcggaatg tcgtctgcgg
     2161 gctcgcactg gtggcggtct gcaccctgtt ggtaccgcct cccgcgcagg ctgcggatct
     2221 gcggggggct ggtggcctgg tcccgggctg gagctggaat tggctcggga gcctctggtc
     2281 tgaggtggga agcgccctga attcgttctt tggagagaat gaggaaactc cggcccctgt
     2341 gtcccgtacc tgggataagc agggcacggg agtcgaccct aacggtcctc cggcgctcga
     2401 gggcggagcc tcgctggtca ccattacgat ctgcgactcg gccccagaga gttgcgcccc
     2461 cgtggctggg cggtagccgg ccttgggttt gcatgtggcg aacgtgagtc cgcttgttct
     2521 ataattagcg gcaccttgct ttgcggtaca ggaaaggaga gtcgagcgtg agcagccatc
     2581 ccagcacagc ggagctgcag gacttcttga gcggtggctt atccgctcgc cagtctcgaa
     2641 ccgtggtgct ccatctgctc cgcggctgtg agagctgccg ccagattgta ggcccgtata
     2701 tgcaggcgct gttgggtgag cagccggtcg aggctccgct ctctgcggag ctggagacgg
     2761 cctatgacgc cgcccttgac cgtgcctttg cagcggcttg cgagttgccc ctgtccaaga
     2821 gcgggtggag cgtggcagtg gagcaggccc tcgtcctctt caagtccagg atttcgacgg
     2881 gagaggctga gacgctacct tcgctggatg aagtggcagc gatcgagacg ctcctcgatc
     2941 tttgccgcga ggtgcgtcac gagaatccga agcggatggt gcagctcgcc caagaggcgg
     3001 tcgctgcggc cggccggctc aatcccgacc accatggcgt gcggcgggtc gcggacctga
     3061 ggtgtcgcgc taccgtcgag ttggggaacg cctaccgcgt cgccgaccgg ctggacttgg
     3121 ctgaagggct ggtggagcaa gccgccgcgt tctacctgga aggtacggga gatccgtttt
     3181 tgggagctcg cctgttcgat gtccaggcct ctctcgacgg cgatcgtcgc cgcttcaagc
     3241 ttgcctgtaa ggcgctggac gcggtccatt ccttccacct ccggcatggg gaccagcacc
     3301 tggcgggccg cgcgctcatc agcaagggac tctacacagg ctacagcggt gactgcgagg
     3361 aggctatcca cctgctgcag agcgctctga ccctggtaga tgccgagcgt gatccggccg
     3421 tggctgccgc aggtctccac aaccaggtct ccttcctcgt ggattgcggc cggttccgtg
     3481 aagctcgggc ccttctctgg cagaaccggg ctgacctgga gcgggtgagt ggttgggtga
     3541 ctcgcctcaa gctccgctgg cttgagggtc agatcaacgc cgggcttggc gatctcgacc
     3601 gggctgagca ggctcttctc gaggttcagg agggattcca gaaggccgac cttcattaca
     3661 aggcagctat cgcttcgctg gatctcgccg tcctctggct acgtcagggc cgggcggacg
     3721 aggctcgcga catcgtcttg aaggccgccg aggtcttcac cgccctcaag atccatcgcg
     3781 agggtctggc cgcggtcctg ctccttcgca aggcgttcga gacccgggtt gccaccacct
     3841 cgctgctgga gcgggtgatc cgcttcatga cccgcgccga acacgatccc agcgtcagct
     3901 tcaaggcctg gttcctgtag gtccggccgg cttgcccacg gcttccgacg gtccgctccg
     3961 caaggggcgg gccgttttgc ttttgagcga atctcactcc ctgtgcaacc ttctctcttc
     4021 ctccgcgtac ctcccccttg accgccaact actacggtag tactatcctg gtagttgaag
     4081 cggaggaggc tacatggaaa cgtcctacca cctcggagat ctgcagctcg cgatcatgag
     4141 ggtcctgtgg agccgcggcg agtcgactgt cgcggacgtc cacgacacgc tggagcccga
     4201 gcgcggcctg gctctcacca ccatcgccac catgctgacc aagatggaga agaagggtgt
     4261 ggtcgaccac cgggccgagg gccgcaagtt catctaccgg cccctggtga gcgagggtca
     4321 ggtccgccgc tcgatggtgg aagacctgac ctcgcagctc ttccggggcg acgtcaccgc
     4381 cctggtcaac cacctgctgt ccgagcacga gatcgaccgc cgcgagctgg cgcagctccg
     4441 cgacctgatc tccgcccacg aggaagcgca cgagacctac gaatccaagg aggagcgctg
     4501 acatgttcga agctctgatg cccacctggg cgagcaccgc ggccgcgtgg ctgctcacct
     4561 atgccctgca cagcacgctg ctcctgggcc tcgcctggct cgtgtcgcgc cgtctggccg
     4621 gacgcgctcc tctcgccgag gaggcgatct ggagaggagc cctgctcggc gccgtgctga
     4681 ccgcctcgct ccagctcgcc ggcggctggg agccgctggc cgggagctgg cggctcgaat
     4741 ccacctctcc tccagccgca gcggcgaccc gcctggagac ggcccccctg cccgccgccg
     4801 cccgagaggt ggccgcccgc ccgttggcac ctcgaacgga tgagccggtc gcggcctccc
     4861 ccgccgcgcc ttctcagttc ccggccggcg cggccgttct cggcctctgg ctcatggggg
     4921 cgttcctgat cggcgcgtgg cacgcccgct cgtacctgcg gctccacc
//
LOCUS       DASPJI010000288         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_311513, whole genome shotgun sequence.
ACCESSION   DASPJI010000288 DASPJI010000000
VERSION     DASPJI010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311513"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1504)
                     /locus_tag="VEL74_07630"
     CDS             complement(<1..1504)
                     /locus_tag="VEL74_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HYG62436.1"
                     /translation="MTILRKIVPLVRRSGTSLRPDASSVRTDGMPRRRIVSSVRLFVP
                     PLRPDASPARPDVTILRKIVPSVRRSGASVRPGASSVRTDGTPRRRIGASVRRFAPSF
                     RSGETARGKIVPSERRVVPSVRPDETVRRKIVPSFPRSEPSLRPGVSPDILIHVTTPP
                     TRPALQPPQKADQASLAKFLKKYRPVLWVGAGASVAANYPGTNDLIREMVDESEVTID
                     PLLPFFEVADRFVGSQGEGALGDLLQRVLGPPRDPASLHLAIARLAKEGYFQAIVTTN
                     YDDLLERTLAAAGVRCAIQPLEQNSTVVADGHIRLLKLHGSRENWADVILSSHSYQSF
                     SHRYPFLVSQLDVLLRQHPVLFIGCSLQDPRILDWLTGLTPDSARALKPWRSLMIQKD
                     WEKAAKAEALTRGNIRPYILDSHGQLGELLQAVAGEVKPDLARLEIEVELGDRWQARM
                     AGVEWETADPLADENLLKDLEQLRDLGLRYLPTDERGHLSPEAAAAAGALR"
     gene            complement(1645..>2245)
                     /locus_tag="VEL74_07635"
     CDS             complement(1645..>2245)
                     /locus_tag="VEL74_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYG62437.1"
                     /translation="DDQVKIRGFRVEPGEIETVLAASPDVAECAVAAREGGARPGDLR
                     LVAYVVPAAGRQASAPDLRAFLEARLPAWMVPAAYVPLTALPRTAAGKIDRRALPAPD
                     LSGLAAVKERPYVAPRNEVEEVIAEIWCEALGVERLSVFDGFFELGGHSLLLLQVMRR
                     LRDAFDLEIPLRSIYEERTVAALAKRVEELLIEEIRQLV"
BASE COUNT          376 a          747 c          801 g          321 t
ORIGIN      
        1 tccggagggc tccggcggcc gcggcggcct cgggggacag gtgtcctctc tcatccgtcg
       61 ggagataccg cagcccgaga tcccggagct gctccaggtc tttcaggagg ttctcatccg
      121 ccagcgggtc cgccgtttcc cactccacgc cagccatccg agcctgccag cggtccccca
      181 gctcgacctc gatctccagg cgggccaggt ccggcttcac ctcgccggcc acggcctgga
      241 ggagctcccc gagttggccg tggctgtcca ggatgtacgg ccggatgttc cctcgggtga
      301 gagcttcggc cttggccgcc ttttcccaat ccttctgaat catcaacgac cgccagggct
      361 tgagggccct cgccgagtcg ggggtcaggc ccgtcagcca gtcgaggatg cgggggtcct
      421 ggagcgaaca gcctatgaag aggacggggt gctggcggag gaggacgtcg agttgggaga
      481 ccaggaaggg atagcgatgg ctgaacgact gataggaatg gctggagaga atgacatcag
      541 cccagttctc tcgtgaacca tggagtttga ggagccggat gtggccgtcc gccaccacgg
      601 tagagttctg ttccagcggc tgaatcgcac agcggactcc ggcagccgcc agagtccgct
      661 ccaggaggtc gtcgtagttc gtggtgacga tggcctggaa gtatccctcc ttggcgagcc
      721 gggcgatggc aaggtggaga gacgcgggat ctcggggcgg acccaggacg cgctggagga
      781 gatcacccag agcgccctcg ccctgagacc cgacaaaacg gtccgccacc tcgaagaagg
      841 gcagcaacgg atcgatggtg acttcggact cgtccaccat ctcgcggatg agatcgttcg
      901 tcccgggata attggcggcc acagaagcac ccgctccgac ccagagaacg ggcctgtact
      961 tcttcaggaa cttggcaagg ctggcttggt ccgccttctg gggcggctgg agggcgggcc
     1021 gggttggcgg cgtggtcacg tgaataagga tatcaggcga gacaccgggc cggagggagg
     1081 gctcagaccg cgggaaggat ggaacgatct tccggcggac cgtctcatcg ggccggacgg
     1141 atggaacgac ccggcgttcc gatggaacga tctttccgcg ggctgtctca ccggaccgga
     1201 aggatggagc aaaccgccga acggatgcgc cgatccgccg gcggggcgtt ccgtcggtcc
     1261 ggaccgatga ggcgccgggg cggacggatg cgccagacct ccggaccgat gggacgatct
     1321 tccggaggat cgtcacgtcg gggcgagccg gtgaagcgtc gggccggagg ggtggaacaa
     1381 acagccggac ggatgagacg atccgccggc ggggcattcc gtcggtccga accgatgagg
     1441 cgtcgggccg gagggatgtg ccagacctcc ggaccaatgg gacgatcttc cggaggatcg
     1501 tcacgtcggg ctgggcgggt gagacggccg gccggcggga tgagacgacg gctcggacgg
     1561 atgacctccc ccgctccctc ccgcccaccc tcccaggggg ggaggcacca ccacccccgc
     1621 aatcctagcg cccctgcaac ccgactagac gagctggcgg atctcctcga tgagcagctc
     1681 ctctacccgc ttggccagcg ccgccaccgt ccgctcctcg tagatgctgc gcagcgggat
     1741 ctccaggtcg aacgcgtccc gcagacggcg catcacctgg aggaggagga gcgagtggcc
     1801 tcccagctcg aagaagccgt cgaagacgct cagccgctcg acgcccagcg cctcgcacca
     1861 gatctccgcg atcacctcct ccacctcgtt gcgcggcgcc acgtagggcc gctccttgac
     1921 cgccgcaagg ccggagaggt ccggcgccgg cagggcgcgg cggtcgatct tgccggccgc
     1981 cgttcgcggc agggcggtga ggggcacgta ggccgcgggc accatccagg cgggcagccg
     2041 ggcctccagg aaggcgcgga ggtccggggc ggacgcctgg cggccggcgg ccggcaccac
     2101 gtaggccacc aggcggaggt cgccgggacg cgcccccccc tcccgggcgg ccaccgcgca
     2161 ctccgccacg tccggcgagg cggcgagcac agtctcgatc tcccccggct cgacccggaa
     2221 gccgcggatc ttcacctggt cgtcc
//
LOCUS       DASPJI010000289         3546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_823519, whole genome shotgun sequence.
ACCESSION   DASPJI010000289 DASPJI010000000
VERSION     DASPJI010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823519"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VEL74_07640"
     CDS             complement(<1..975)
                     /locus_tag="VEL74_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG62438.1"
                     /translation="MVKKTGHSLLAIAGLALGTTCLLFALLYVREELSYDRFHEKADR
                     IYRISQDLTPPSGEVVNTVFTQIIMAPTLIKDFPEMAEVARIMPWFEGAVPGKVAVRR
                     QEKGQEKQSYEWFAWADGSLPRIFDLPLIEGDPAKVLVAPNSVVLSEPIARKIFGDEP
                     AVGKVLKIDSGYSDENYRVTGVMRELPSNSHFHFHVLASFSSLDHVTDPRLIRDNWWA
                     NDVYTYFLLAEGASAEQIEKKLPAFVEKYYPKVPGASAAMRLQRLRDIHLHSDQFGEI
                     EPGGDPRNVKIAAAVALFVLLITALTFLVLTAPRPSFKRALTLAAAGAA"
     gene            complement(984..>3546)
                     /locus_tag="VEL74_07645"
     CDS             complement(984..>3546)
                     /locus_tag="VEL74_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG62439.1"
                     /translation="ETIRAEGITTMALPPPILATIPPENLPSLCTILVGGDRCSADLA
                     NRWVPPGSTLRLLNCYGPTETTIYSGFQRCAGPYRRDPSIGRPIANLHMAVLDPRGRL
                     APVGVPGELCVGGAGTVRGYLHRPDLTAQRFVPDPFGGEPGGRLYRSGDLVRLLPHGE
                     IEFLGRIDRQVKLRGLRIELGEIEALLAAHPAVRECAVVVREDAPGEKALVAYLVPQA
                     EPAPEASGLRTFLAAKLPSYMVPTAFVTLEALPLSPTGKVDHAALPAPSRERSGGDVV
                     AARNQAEATLAGIWSQVLGVEKVGIHDNFFDLGGDSILCIQVVSRAQQEGLWLTARDL
                     FRHQTVAELAALAATAGAGPEASVDEEAGAEVPLTPVQRLFFDLDLEDPHHFNQAVLL
                     ALQQEADPGRLERALGAVAALHSALRYRYLRDGEGQWHQVATDMAAPLAVLDLSALPQ
                     ARRRAALEGAAARLQPSLDLTRGPLLRAALFALGEEGQRLLLVLHHLIVDGVSWRILL
                     GDLETAYRSLAAGASPALPPVATPFARWAAKLAEHAGSAAAKEELPYWLAPARAAVLP
                     LPVDLPGGAADDTLGSAGSVIAELDPEATRALLQDVPKAYHTQINDVLLTALAEAFAG
                     WTGQPHLLVDLEGHGREELFEGFDVSRTVGWFTSLFPVLLQLPDSDSAAAGPGDHLKA
                     VKEQLRAVPRGGVGYGLLRYAGDEEAAGRLREMPPAEVVFNYLGQLDRGLAGSALFAP
                     AGESSGPALSPRQRRGHLLEINGGIVATDAGSSLRLTWTYGVNRHHRHTIQALADRFI
                     ASLRGLIEHCRAKESGDFTPSDFPEARFSQKDLDTLMARIGKRSPGAGARRTGPR"
BASE COUNT          520 a         1237 c         1311 g          478 t
ORIGIN      
        1 ggccgctccg gccgccgcga gggtgagggc ccgcttgaaa gagggccgcg gcgccgtcag
       61 caccaggaag gtgagggcgg tgatcagcag gacgaagagg gcgaccgccg cggcgatctt
      121 cacgttccgg ggatcgccgc caggctcgat ctcgccgaac tgatcggaat gcaggtggat
      181 gtcgcgcagg cgctggagcc gcatggcggc cgaggctccc gggaccttcg gatagtactt
      241 ctcgacgaaa gccggcagct tcttttcgat ctgctcggcg ctggcgccct cggcgagcag
      301 gaaataggta tagacgtcgt tcgcccacca gttgtcacgg atcagccgcg ggtcggtgac
      361 gtgatcgagg ctggagaacg aggcgaggac gtggaaatga aaatgcgagt tggacggcag
      421 ctctctcatc actcccgtca cgcgataatt ttcgtcgcta tagccgctgt cgatcttcag
      481 caccttcccc accgccggct cgtcgccgaa gatcttgcgc gcgatgggct cgctcaggac
      541 cacggagttg ggcgccacga ggaccttggc cggatcgcct tcgatcagcg gcaggtcgaa
      601 gatgcgcggc aggctgccgt cggcccaggc gaaccattcg taagactgct tctcctgccc
      661 cttctcctgg cggcggacgg cgaccttgcc cgggaccgcg ccctcgaacc agggcatgat
      721 ccgcgccacc tcggccatct cgggaaaatc cttgatcaag gtgggcgcca tgatgatctg
      781 ggtgaagacg gtgttcacca cctcgccgct cggcggcgtc aggtcctgcg aaatgcgata
      841 gatccggtcg gccttctcgt ggaaccggtc gtagctgagc tcctcccgca catagaggag
      901 ggcgaacagg agacaggtcg tgccgagggc gagcccggcg atggcgagga gagaatgtcc
      961 ggttttcttg accatggtcc ctttcagcga ggcccggtgc gccgcgcccc ggctccaggg
     1021 ctgcgtttgc cgatgcgggc catcagcgta tccaagtcct tctgactgaa ccgggcctcc
     1081 gggaagtccg acggcgtgaa gtcgcccgac tccttggcgc ggcagtgttc gatcagcccg
     1141 cggaggctgg cgatgaaacg gtcggcgaga gcctggatgg tgtggcgatg gtggcggttc
     1201 acaccgtagg tccaggtcag ccgcaagctg ctgccggcgt cggtggcgac gatgccgccg
     1261 ttgatctcca gcaggtggcc gcgcctctgg cgcgggctca gcgccgggcc gctcgactcg
     1321 ccggcggggg cgaacagcgc cgagccggcg aggccgcggt ccaactgccc caggtagttg
     1381 aagaccacct cggcgggcgg catctcgcgc agccggccgg ccgcctcctc gtcgccggcg
     1441 tagcgcagca ggccgtagcc gaccccgccg cgcggcaccg cccgcaactg ctccttgacc
     1501 gccttgaggt gatcgcccgg gccggccgcc gcgctgtccg agtcggggag ttggagcagc
     1561 accgggaaga ggctggtgaa ccagcccacc gtgcgcgaga cgtcgaagcc ctcgaacagc
     1621 tcctcccggc cgtgcccctc gaggtccacc agcaggtggg gctgccccgt ccagccggcg
     1681 aacgcctcgg ccagcgcggt cagcaggacg tcgttgatct gggtgtggta ggccttcggc
     1741 acgtcctgga gcagggcgcg ggtggcctcc ggatccagct cggcgatcac cgagcccgcc
     1801 gaccccaggg tgtcgtccgc cgcgccgccc gggagatcga ccggcagggg gaggacggcg
     1861 gcgcgcgcgg gggcgagcca gtagggcagc tcctccttcg ccgcggccga ccccgcgtgc
     1921 tcggccagct tcgccgccca gcgcgcgaac ggggtggcga cgggcggcag cgcgggagag
     1981 gccccggcag ccagcgagcg ataggcggtc tccaggtcgc ccagcaggat gcgccaggag
     2041 acgccgtcca cgatcaggtg gtggaggacc aggagcagcc gctgcccctc ctcgcccagc
     2101 gcgaagagcg cggcgcgcag cagcggcccc cgcgtcaggt cgaggctcgg ctggagccgg
     2161 gcggccgccc cctccagagc cgcccgccgc cgcgcctggg gcagggccga gaggtcgagg
     2221 acggccaggg gcgcggccat gtcggtcgcg acctgatgcc actgcccctc gccgtcgcgc
     2281 aggtagcgat agcgcagcgc cgagtgcagg gcggccacag cgccgagcgc ccgctccagc
     2341 cggccgggat ctgcctcctg ctgcagagcg agcagcaccg cctggttgaa gtggtgcggg
     2401 tcctcgaggt cgaggtcgaa gaagagccgc tggaccgggg tcagggggac ctcggcgccg
     2461 gcctcctcgt ccacggaggc ctccggcccc gctccggccg tcgccgcgag ggccgcgagc
     2521 tcggccaccg tctggtgccg gaacaggtcg cgggcggtca gccagagccc ctcctgctgc
     2581 gcccgcgaga cgacctggat gcacaggatg gagtcgccgc ccaggtcgaa gaagttgtcg
     2641 tggatgccga ccttctcgac gcccagcacc tgcgaccaga tccccgccag ggtggcctcg
     2701 gcctggttcc gcgccgccac gacgtcgccg ccggaccgct cgcgggacgg ggcggggagg
     2761 gccgcgtggt cgaccttgcc cgtcggggac agggggagcg cttccagggt gacgaaggcg
     2821 gtcggcacca tgtaggaggg gagcttcgcc gccaggaagg tgcgcaggcc ggaggcctcc
     2881 ggcgccggct ccgcctgcgg cacgagatag gcgaccagcg ccttctcgcc gggcgcgtcc
     2941 tcgcgcacca ccaccgcgca ctcccgcacc gccgggtgcg ccgccagcag cgcctcgatc
     3001 tcccccagct cgatgcgcag gccgcgcaac ttgacctggc ggtcgatccg gcccaggaac
     3061 tcgatctcgc cgtggggcag cagccgcacc aggtcgcccg agcggtagag acgcccgccc
     3121 ggctcaccgc cgaaaggatc ggggacgaac cgctgcgccg tcaggtcggg gcggtgcagg
     3181 tagccgcgca ccgtgccggc gccgccgacg cacagctcgc ccggcacccc caccggcgcc
     3241 aggcgcccgc gcgggtcgag caccgccatg tggaggttgg cgatcggccg gccgatgctc
     3301 gggtcgcgcc ggtaggggcc ggcgcagcgc tggaagccgg agtagatcgt cgtctcggtc
     3361 ggcccgtaac agttgagcag ccgcagggtg ctgcccggcg gcacccagcg gttcgccagg
     3421 tcggcggagc agcggtcgcc gcccaccagg atggtgcaca gggagggcag gttctcgggg
     3481 gggatcgtcg ccaggatcgg cggcggcagc gccatggtgg tgatcccctc ggcgcgtatg
     3541 gtctcg
//
LOCUS       DASPJI010000290         2821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_418053, whole genome shotgun sequence.
ACCESSION   DASPJI010000290 DASPJI010000000
VERSION     DASPJI010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418053"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VEL74_07650"
     CDS             <1..234
                     /locus_tag="VEL74_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HYG62440.1"
                     /translation="PRWAALVPRGGEGLYSASLAGTLVLAVGAEGPGLSPALGERAGL
                     RLTIPIEPPVESLNATVAAALVLFEWRRRRLGS"
     gene            268..756
                     /gene="rpiB"
                     /locus_tag="VEL74_07655"
     CDS             268..756
                     /gene="rpiB"
                     /locus_tag="VEL74_07655"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HYG62441.1"
                     /translation="MKIAIGSDHAGYTYKEAIKDYLLSRGHEVRDFGTHSEDPVDYPL
                     FIRPVAEAVSRGEVDRGIVLGGSGNGEAMVANRVPGVRCALCWNVETAELGRRHNNAN
                     ALSLGERMMPLETALRIVEIWLETPFDGGRHVRRVQMIDQEPAQVPEGERGAPAAETL
                     RA"
     gene            753..1685
                     /gene="murB"
                     /locus_tag="VEL74_07660"
     CDS             753..1685
                     /gene="murB"
                     /locus_tag="VEL74_07660"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HYG62442.1"
                     /translation="MSAALLQALGPDRVRRGEILAPYTTFRIGGPADLFYEAENAEEL
                     ARAILAAREHGAPFFLLGLGANILIGDRGFRGLVIRNRARGVEVRPAEGLVRAESGAV
                     VWPDLIETAIAQGLSGLEHYVGIPSTVGGALWQNLHFLAPAPARERTMFIAEVTHEAE
                     ILTAEGERQTVGVDYFEFGYDQSILHHRPDVVLAATFQLTPEEPERMRRIVEENLQWR
                     RERHPPLDTEPSAGSIFKKIQGIGAGRLIDECGLKGTAIGGAMVTPRHANIMINRGGA
                     TAADVRALIAHVQEVVERGTGYRLEPEISFVGEF"
     gene            complement(1695..2765)
                     /locus_tag="VEL74_07665"
     CDS             complement(1695..2765)
                     /locus_tag="VEL74_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019011874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG62443.1"
                     /translation="MKLQLKKLADQVMVITGASSGIGLVTAKQAAAAGARVVLAARNE
                     DELAAATQEIRQNGGQALHVRADVTDPAQVEEIAEAAIRAFGRIDTWVNNAGVAMYGR
                     LTDVALEDARRLFDVLFWGVVHGSRTAVAHMSHDGGALINVASIVADRAVPLQGIYSA
                     AKHAVKAFTETLRMELEEEGTPISVSLIKPSSIDTPLFDKARTLLEFEPRPVPPVYAP
                     EVVAETILECAQRPIRDVIPGGMGKVLSTSGRIAPRLTDRYMERTLFRQQRTDEPAGG
                     RPDNLFTPVEQDGGERGRNYKGRTTERSLYTKASLHPGAAALSALGLGAMLLAGSRVL
                     KARRRRAARWEEEGGLVLPKDL"
BASE COUNT          381 a         1041 c          996 g          403 t
ORIGIN      
        1 ccgcgctggg ccgccctggt gccgcggggg ggcgagggcc tctactccgc ctccctcgcc
       61 ggcaccctgg tcctcgccgt gggggccgag gggcccggcc tctcccccgc cctcggcgag
      121 cgggccggcc tccgcctgac catccccatc gagccgcccg tggagtcgct gaacgcgacg
      181 gtggcggcgg ccctggtgct gttcgagtgg cggcggcgca ggctcggctc gtgatctaat
      241 gcggccgaca gcccagaggg ggagagcatg aagatcgcca tcggttccga ccatgccggc
      301 tacacctata aggaagccat caaggactac ctgctgagcc gcggccacga ggtccgggat
      361 ttcggcaccc actccgagga cccggtggac tatccactgt tcatccggcc ggtggccgag
      421 gcggtgtccc ggggagaggt ggaccgcggc atcgtcctgg gcggctcggg caacggcgag
      481 gccatggtgg ccaaccgcgt ccccggcgtc cgctgcgccc tctgctggaa cgtggagacc
      541 gcggagctgg gccgccgcca caacaacgcc aacgccctct ccctgggcga gcgcatgatg
      601 cccctggaaa cggccttgcg gatcgtcgag atctggctcg agaccccctt cgacggcggc
      661 cgccacgtgc ggcgggtgca gatgatcgac caggagcccg cgcaggtccc cgagggcgag
      721 cggggggcgc cggcggcgga gaccctgcgg gcttgagcgc ggcgctcctc caggccctgg
      781 ggccggaccg cgtccggcgg ggcgagatcc tcgcccccta caccaccttc cggatcggcg
      841 gcccggccga cctgttctac gaggcggaga acgccgagga gctggcgcgg gccatcctcg
      901 ccgcccgcga gcacggggcc cccttcttcc tcctcgggct gggagccaac atcctcatcg
      961 gcgaccgcgg gttccgcggc ctggtgatcc gcaaccgcgc ccgcggggtc gaggtgcggc
     1021 ccgcggaggg gctcgtccgc gcggagagcg gcgccgtggt ctggcccgac ctcatcgaga
     1081 ccgccatcgc ccagggcctc tccggcctgg agcactacgt gggcatcccc tcgacggtgg
     1141 gaggggcgct ctggcagaac ctccacttcc tcgcccccgc cccggcacgg gagcggacca
     1201 tgttcatcgc cgaggtgacg cacgaggcgg agatcctcac cgccgagggg gagcggcaga
     1261 cggtgggggt ggactacttc gagttcggct acgaccagtc catcctccac caccggccgg
     1321 acgtcgtcct cgccgccacc ttccagctca cgccggagga gccggagcgg atgcggcgga
     1381 tcgtcgagga gaacctccag tggcgccgcg agcgccaccc gcccctcgac accgagccgt
     1441 ccgccggctc catcttcaag aagatccagg ggatcggcgc cgggcgcctg atcgacgagt
     1501 gcggcctcaa ggggacggcg atcggcggcg ccatggtcac gccccgccac gccaacatca
     1561 tgatcaaccg gggcggtgcc accgccgccg acgtccgcgc cctcatcgcc cacgtccagg
     1621 aggtcgtgga gcgagggacc ggctaccgcc tggagcccga gatctccttt gtcggcgagt
     1681 tctgaggccc ctcctcagag gtccttcggc agcaccagcc caccctcctc ctcccaccgg
     1741 gcggcccggc gccgcctggc cttgagaacc cgactgccgg cgaggagcat ggcccccagc
     1801 cccagagcgg agagcgccgc cgccccgggg tggagcgacg ccttggtgta gaggctccgc
     1861 tccgtggtcc gccccttgta gttgcggccg cgctcgccgc cgtcctgctc caccggcgtg
     1921 aaaaggttgt ccggccggcc gcccgcgggc tcgtcggtcc gctgctgccg gaagagcgtc
     1981 ctctccatgt aacggtcggt caggcgcggc gcgatacgtc cggaggtgct gaggaccttc
     2041 cccatgccgc ccgggatgac gtcgcggatc ggcctctggg cgcactccag aatggtctcg
     2101 gcgaccacct ccggcgcgta gacggggggc accggccggg gctcgaactc caggagggtg
     2161 cgcgccttgt cgaacagcgg cgtgtcgatg ctgctgggct tgatgagcga cacggagatg
     2221 ggtgtcccct cctcctccag ctccatgcgc agagtctcgg tgaacgcctt caccgcgtgc
     2281 ttggcggcgc tgtagatccc ctggagcggc acggcccggt cggccacgat gctggcgaca
     2341 ttgatcaggg cgccgccgtc gtggctcatg tgcgccaccg ccgtgcggct gccgtggacc
     2401 acgccccaga agaggacgtc gaacaggcgg cgcgcgtcct ccagcgccac gtcggtgagg
     2461 cgcccgtaca tggcgacacc ggcgttgttg acccaggtgt cgatccgccc gaaggcccgg
     2521 atggccgcct cggcgatctc ctcgacctgc gccggatcgg tgacgtcggc gcggacgtgg
     2581 agggcctggc cgccgttctg ccggatctcc tgggtggcgg cggccagctc gtcctcattg
     2641 cgggcggcga gcaccaccct cgccccggcg gcggcggcct gcttggcggt caccaagccg
     2701 atgccgctcg acgcgccggt gatgaccatc acctggtccg cgagcttctt gagttgcagt
     2761 ttcatggcgt ctccttcctg tgagccgtgc aaaaggtcag cacggaggat gccaacggcc
     2821 c
//
LOCUS       DASPJI010000291         4952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_251026, whole genome shotgun sequence.
ACCESSION   DASPJI010000291 DASPJI010000000
VERSION     DASPJI010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251026"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="VEL74_07670"
     CDS             complement(<1..164)
                     /locus_tag="VEL74_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013556905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYG62444.1"
                     /translation="MDSIVVRGAREHNLKNVNLEIPRNQLVVITGVSGSGKSSLAFDT
                     IYAEGQRRYV"
     gene            complement(334..951)
                     /locus_tag="VEL74_07675"
     CDS             complement(334..951)
                     /locus_tag="VEL74_07675"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018978866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HYG62445.1"
                     /translation="MDRFRPLPMSFYRRPAEEVARDLLGRYLVREVDGERLVLRLVET
                     EAYLGGPDRASHAWGGRRTVRNESLYLPGGHAYVYFIYGMHWCLNAVTGEADIGSAVL
                     LRAGEPVEGEARMVENRRWARKVKPGDLAGGPGKLCQALAIDRTLDAVRLDRGALRIT
                     AGESVGESEVVAGPRIGVDYAGEAAAWPLRFAVRGHLHVSRPRPW"
     gene            1185..1886
                     /locus_tag="VEL74_07680"
     CDS             1185..1886
                     /locus_tag="VEL74_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HYG62446.1"
                     /translation="MAANPLPRLEIPALFEELPDAFASRLDPEAPWSLLGDPLDEILG
                     ALPSEDVRIRVLPEVHLTGDRIVICPGTHIHPTAVIEGPVFIGRDVEIRPGAYIRGGV
                     WIGDGCVVGANTELKRAILLPHAKAPHLNYVGDSILGRDVNLGAGTILSNFRHDGRKV
                     RIAANGHPIASGRRKLGAVLGDGVQTGCNCVLHPGVVVGRGTQIYPGVQLRSGLYPER
                     SIVKLRQELDVVPQS"
     gene            complement(1883..3043)
                     /locus_tag="VEL74_07685"
     CDS             complement(1883..3043)
                     /locus_tag="VEL74_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62447.1"
                     /translation="MDRGIQAERLAALGASPSEIEELLVYNENRFDVEALGPETEIPP
                     SDEPFVSLWEGWVEEARERGAFAVLAGHLPQLRFPIQEGISETEAYRAATRKGGSPDE
                     LPEATGLVIERPERIELVLYTSPAGRIPLIIARDRQAFVALNRALGKRNEPVPLQESQ
                     GALMISGYNNWERIHALRRRWESLDPAERETATWNEEMARILPRRELYQDRFILLSDG
                     PYSAVPATELGLEATEWREASLTIRRDHECAHYFTRRLLGSMRNNLLDELIADYAGMT
                     AATGCFRAEWFLRFLGLFPEYGPGARVDVYRGDPPLSDGAFRVLHGLIACAAESVERF
                     DAKLKGPLDVREKALRIATLASFRLEDLAEPGAENLLGRRLEELRGPDQRSI"
     gene            3122..4660
                     /locus_tag="VEL74_07690"
     CDS             3122..4660
                     /locus_tag="VEL74_07690"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYG62448.1"
                     /translation="MNPSGWSRFSAGLALTALLAAAPAAAQSPEPGLGLVEAVRMMLG
                     TDPNLSLEQARVRSARGLLLSTRGEFDPVLATSVGEEEITNPISERSSEEQRILTNSL
                     GVTKRFRTGLSVEPGLSLSRTEETDAVNVGTFSFTVRQPLLRGRGRDVTMAPELSAER
                     EVAASELDLRQTTAERMLAVVSQYWQTRAAALNLEILQENEDRSRELLETTRKLIEAD
                     VTPAAELVQVEANLVAAEASRIGGERLLFEAQRDLGREIGLDRASIAALRLPTDPFPT
                     VPAAAVPAASNGERFVAAALDRRADLRAARERRNAAEVLLRAADDGLKPRLDLVWIPS
                     YTGLVEGNDAGSFFSPLYRNVPGASSSFGLTLSWPTLNSQARGERIRIEAAREQSLLF
                     EDLVARQIGADVPTALDAVQREAQRVERATTAIRLYEQTVINEEKKLRAGSSTLIDLI
                     SQRDRLTAARQSQVSANLALALALARLRFETGTLPVEGEPGPNWFGELTSVPSPDAPR
                     EVAP"
     gene            4657..>4952
                     /locus_tag="VEL74_07695"
     CDS             4657..>4952
                     /locus_tag="VEL74_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HYG62449.1"
                     /translation="MIFRKVALERLSSPEQLDQLMQVTNPRGWLALAALGALLLTAVF
                     WGVFGSIPTEAAGEGILLRRGGVSNLVAPESGQVQEILVAVGDEIQKGQVVARLR"
BASE COUNT          761 a         1794 c         1662 g          735 t
ORIGIN      
        1 tccacgtagc ggcgctggcc ctcggcgtag atggtgtcga aggccaggga cgacttaccc
       61 gagccggaga ccccggtgat gaccaccagc tggttcctgg ggatctccag gttgacgttc
      121 ttcaggttgt gctcgcgggc accgcggacg acgatgctat ccataggaga gggcatccta
      181 gcagaaattg aggcgtaagc tgtaggctcg tgatggttgc ggctggcgcg gccgtccaat
      241 ccaacacacc ggaccggcgc cgtgttcccg gggagcctac cacacccgga gttccgggac
      301 ccggcgaatt gtctcgccga gagggcctcg gattcaccag ggccggggcc gcgacacgtg
      361 cagatgtccc cggaccgcga agcgcagagg ccaggccgcg gcctcgccgg cgtagtcgac
      421 gccgatgcgg ggaccggcca cgacctcact ctcccccact gactcgccgg ccgtgatgcg
      481 gagcgccccc cggtcgagcc gcacggcgtc gagcgtgcgg tcgatggcga gggcctggca
      541 gagcttgccc gggcccccag ccaggtcccc cggcttcacc ttgcgggccc accgccggtt
      601 ctctaccatc cgcgcctccc cctctacggg ctcccccgcc cgtaacagta ctgcgctgcc
      661 gatgtccgcc tcgccggtga cggcgttcag gcaccagtgc atgccataga tgaaatagac
      721 gtaggcgtgc ccacccggca gatacagact ctcgttcctc acggtccgcc gtccccccca
      781 cgcatggctg gcccggtccg gcccgcccag atacgcctcc gtctccacca gccgcaggac
      841 cagccgctcc ccgtcgacct cccgcaccag ataacgtcca agcagatcgc gggcgacctc
      901 ctcggcgggg cggcgataga aagacatggg aagagggcga aagcggtcca tgaggaagcg
      961 acacgataca ccgtccccgt aggcctgggg gagtcttccc gtcccggcgc aggcgactgg
     1021 cagcgtggga ggggggcggg tggagcgcac acgaaggagc cgagcaggga cgggacgact
     1081 cccccaggcc gtggcacatc ccttgctccc aagatctctg ccggttagag cccgcgtata
     1141 cttcgcgcac gccacgacag tcgaggatgg aggtaaatcc gcccatggcc gccaatccgc
     1201 tcccgcgtct cgagatcccc gcgctcttcg aagagctgcc cgacgccttc gcctcccgtc
     1261 tcgacccgga ggccccgtgg tccctcctcg gcgatccgtt ggacgagatc ctcggcgctc
     1321 ttccttcaga ggacgtgcgc atccgcgtcc tgcccgaggt ccacctgacc ggcgaccgga
     1381 tcgtgatctg cccggggact cacatccacc ccacggctgt catcgagggg ccggtcttca
     1441 tcggccggga cgtggagatc cggccgggcg cctacatccg cggcggggtc tggatcggcg
     1501 acggctgcgt cgtgggggcg aacacggagc tcaagcgggc gatcctcctc ccccacgcca
     1561 aggcgccgca cctcaactac gtcggcgact ccatcctcgg gcgggacgtg aacctggggg
     1621 cgggcaccat cctctccaac ttccggcacg acggccggaa ggtgcggatc gccgccaacg
     1681 gtcaccccat cgcgagcggc cgccgtaagc tgggagcggt gctcggcgac ggggtccaga
     1741 ccggctgcaa ttgcgtcctc caccctggcg tggtggtggg acgggggacc cagatctacc
     1801 ccggcgtgca gctccgctcc ggcctctacc cggagaggag catcgtcaag ctccggcagg
     1861 agctggacgt ggtgccgcaa tcctagatcg atcgctgatc cggcccccgc agctcctcca
     1921 gcctccgccc gaggaggttc tccgcgccgg gctcggctag atcctctaga cggaacgagg
     1981 ccagggtcgc gatccgcaac gccttctccc gaacatccag cggtcccttc agctttgcgt
     2041 cgaaccgctc gacgctctcg gcggcgcagg cgatcagccc gtggagcacc cggaacgctc
     2101 cgtcagacag cggcgggtcc ccccgataga cgtccacacg tgccccgggc ccatactccg
     2161 gaaagagacc gaggaagcgc aggaaccact ccgcccggaa gcacccggtg gcagccgtca
     2221 tccccgcgta gtcggcgatc agctcgtcca gcaggttgtt gcgcatggag cccaggaggc
     2281 gccgggtgaa gtagtgggca cactcgtggt cccggcggat ggtgagcgag gcctcgcgcc
     2341 actccgtcgc ttccagcccc agctccgtcg ccgggacggc gctgtagggt ccgtcgctca
     2401 gcaggatgaa gcggtcctgg tagagctccc ggcgagggag gatccgggcc atctcctcgt
     2461 tccaggtggc ggtctcccgc tcggccgggt cgagggactc ccaccggcgg cgcagcgcgt
     2521 ggatccgctc ccagttgttg tagccggaga tcatgagggc gccctgggac tcctggagcg
     2581 ggaccggctc gttgcgcttt cccagggctc ggttgagcgc cacgaacgcc tgccggtcgc
     2641 gggcgatgat cagggggatc cgtccggccg gactggtata gaggaccagc tcgatccgct
     2701 ccggacgctc gatcacgagg ccggtggcct cgggcagctc gtccggtgag ccccccttgc
     2761 gggtagcggc ccggtacgcc tcggtctcgc tgatcccctc ctggatggga aaccggagct
     2821 gcggcaggtg cccggccagc accgcaaacg ccccccgctc gcgcgcctcc tccacccagc
     2881 cctcccacag cgacacgaac ggctcgtcgg aaggaggaat ctccgtctcc ggccccaacg
     2941 cctccacgtc gaaacggttc tcgttgtaga cgagcagctc ttcgatctcg gagggagatg
     3001 ctcccagggc cgccagccgc tccgcttgaa tccctctgtc cacggtgcta gcctagctca
     3061 gcctggtccg gcaaatgctg gtgagctgta aatttcggaa aaggacggac gggccttgtt
     3121 catgaatcct tccgggtggt cacggttcag cgccgggctc gccctgaccg ctctgctcgc
     3181 cgccgcgccc gcggcggctc agtcccctga gcccgggctc ggtctcgtcg aggccgtccg
     3241 gatgatgctg ggcaccgatc ccaacctgtc gctggaacag gcccgcgtac gctcggcccg
     3301 gggcctgctg ctcagtacgc gcggggagtt cgacccggtg ctggcaacca gcgtcggtga
     3361 agaggagatc acgaacccca tcagcgagag gtccagcgag gagcagagga ttctcaccaa
     3421 ctctctgggc gtcaccaaac gcttccgcac aggcctgtcc gtggagcctg gcctgtccct
     3481 gtcgcgcacc gaggagacgg acgcggtgaa cgtcggtacc ttctcgttca cagtgcgcca
     3541 gccgctgctg cgcgggcgtg gccgggacgt gaccatggcg ccggagctgt ccgccgaacg
     3601 ggaggtggcg gcgagtgagc tcgacctgcg gcagaccacc gcggagcgca tgctggcggt
     3661 ggtctcccag tactggcaga cgcgggcggc ggcgctcaac ctggagatcc tgcaggagaa
     3721 cgaggacagg tcccgggagc tcctggagac cacccgcaag ctcatcgagg cggacgtcac
     3781 cccggcggcc gagctggtac aggtggaggc gaacctggtc gccgccgagg cctcgcgcat
     3841 cggcggcgag cgcctcctct tcgaggcgca gcgcgacctc ggccgcgaga tcggcctcga
     3901 ccgcgccagc atcgccgccc tccgcctccc gacggacccc ttccccaccg tccccgccgc
     3961 cgccgtgccc gctgcgtcca acggtgagcg cttcgtggcc gccgccctgg accgccgggc
     4021 cgacctgcgg gccgcgcggg agcgacgcaa cgcggccgag gtcctgctgc gcgccgctga
     4081 cgatggcctg aagccgcggc tcgacctggt ctggatcccc agctacaccg gcctcgtcga
     4141 ggggaacgac gcggggagct tcttttcccc gctctatcgc aacgtgccgg gagccagctc
     4201 gagcttcggc ctgaccctct cctggccgac gctcaacagc caggcccgcg gcgagcgcat
     4261 ccgcatcgag gccgcccgcg agcagagcct cctcttcgag gacctggtgg cccgtcagat
     4321 cggcgccgac gtccccacgg cgctcgacgc ggtccagcgc gaggcccagc gggtcgagcg
     4381 cgccacgacg gcgatccgcc tctacgagca gacagtgatc aacgaggaga agaagctgcg
     4441 ggctgggagc tccaccctca tcgacctcat cagccagcgc gaccggctga ccgctgcccg
     4501 gcagtcgcag gtctcggcca acctcgcctt ggctctcgcc ctggcgcggc tgcgcttcga
     4561 gacgggtacc ctaccggtag agggcgagcc ggggccaaac tggttcgggg agctcaccag
     4621 cgtcccctcg ccggacgcgc cccgggaggt agcgccatga tcttccgcaa ggtagccctg
     4681 gagaggctgt cgtcccctga gcagctcgat cagctcatgc aggtcacgaa tcccagaggc
     4741 tggctggcgc tcgcggccct tggtgccctg ctgctcaccg cggtcttctg gggggtcttc
     4801 ggctcgatcc ccaccgaggc ggcgggcgaa gggatcctgc tccggcgcgg cggtgtctcg
     4861 aacctcgtgg cgccggagag cggtcaggtg caagagatcc tggtcgcggt gggcgacgag
     4921 atccagaagg ggcaggtggt ggcccgcctc cg
//
LOCUS       DASPJI010000292         3623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_704495, whole genome shotgun sequence.
ACCESSION   DASPJI010000292 DASPJI010000000
VERSION     DASPJI010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704495"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..2283
                     /locus_tag="VEL74_07700"
     CDS             244..2283
                     /locus_tag="VEL74_07700"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HYG62450.1"
                     /translation="MTSPPEPRNRRALLIGIDRYPLLSEAEHLDGAVNDAKAMAQLLT
                     ERFGFPEKAVRLLLDQAATRDAILGAMDRLVEETAKDDVVVFHFSGHGSQVKDLEGDE
                     EDGWDETIVPVDSGREPHPNRQITDDEIYSWLLRLTEKTPYVTLIFDCCHSATITRDD
                     FGGKVRGIERDPAAVREPSPWAGRAQTERVRKGESGWLPWDRRWTLFAGCANHEESHE
                     MRAPGAKGVRHGALTHFLVEELWKAKPGETYRDIFERVEPQVMSVFRSQQPQLEGVWD
                     REVFGLRHFEPLRFVPVLGRHRDGRVLLGAGASSGITIGSRWEIHPPKAQDVVPGTEL
                     GLVEITGVCAFTSEGRIVREARPDFVGRECRAVERAHSHGDLGWAVDLVIPHAWKEHI
                     GILEDGIRESPLLRPASGEEAHTRAYLLGPRRAAGPGDPAPMLETLEEPCWVAVGKDG
                     RLVLPVRRATAPESAFDLLENLEKLARFHHALAFDNPTSPLRGKVDLVVKRLTDGGPV
                     PARQDDGQTAFYEGELLALEAINRTGQKLHLYAIDFGLSGKVSLLYPPYGTREATSRE
                     TLSINTRLGQEWQLTVPDAFPFEAPGTAASGVEFVKLFATTEPVDFKLLFQESFRRDG
                     DFLPGIDDHPLGRLLSEAVWGITYRGPLPGGAARTAADWTTATRSFVLRRRTRAW"
     gene            2764..>3623
                     /locus_tag="VEL74_07705"
     CDS             2764..>3623
                     /locus_tag="VEL74_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG62451.1"
                     /translation="MIGEIYQDLRFCFRSLFRRPSAAIVAAVVAMAIGIGAAVAIFSV
                     VKTVLLQPLPYPNADRAVTVWNQFIQLDLPQVQLSEPELYEYVERSQSLQALAAFVVG
                     QGNLTGVDDPERVDYAAVSPQLFSILGVKASQGRVFVEGEDRPDAPRIIVLSDAFWRR
                     RQGADPNAVGRDMYVNGNRYTIIGIMPPGFRFPEKIDLWLPLRLDPANPGMRGNHYLE
                     ALGQLKPGVTLSQAQAELDVIAQQLQKEFPMSYPADSGWGVNLIPLYEDMTGEVRPAL
                     LIFLAAVGLVL"
BASE COUNT          587 a         1231 c         1204 g          601 t
ORIGIN      
        1 ccttcggcta cggccccggt cagctcgccg gcttcgcgct cggagccctg cgcgcctcct
       61 tcctgccgga cgatgagaag gccgccctcg acgagcgctt ccgccgccag ctcgacgagc
      121 tctccggcga agtcttcggc acccccctcg aagtgccccc ctcctgaggc tgtccaccta
      181 cacaacccac tacgaaaggg gtctctagaa aagacggcgc gcgccgaaag gagacccctg
      241 tccatgacct ctcctcccga gcccaggaac cggcgggctc tgctgatcgg gatagaccgc
      301 taccctcttc tatccgaagc cgagcatctc gatggcgcgg tcaacgacgc caaggccatg
      361 gcccagttgc tgacggagag gttcgggttc ccggagaagg ccgttcggct cctgctcgat
      421 caggcagcca cccgggacgc catcctcggg gcgatggacc ggctggtgga ggagacggcc
      481 aaggacgacg tggtggtctt ccacttcagc ggtcacggct cccaagtcaa ggacttggag
      541 ggggacgagg aggacggctg ggacgagacc atcgttcccg tggattccgg gcgcgagcct
      601 catcccaacc gccagatcac cgacgacgag atctactcct ggctcctgcg gctgaccgag
      661 aagaccccct acgtcaccct gatcttcgac tgctgccatt cggccacgat cacccgcgac
      721 gacttcggag ggaaggtccg cggcatcgag cgggaccccg cggcggtccg ggagccgtcg
      781 ccctgggctg ggcgcgcgca gacggagcga gtgcggaaag gggagagcgg ctggcttccc
      841 tgggaccgtc gctggaccct cttcgccggc tgtgccaacc atgaggagag ccacgagatg
      901 cgagcgccgg gcgccaaggg cgtacggcac ggcgcgctga cccacttcct cgtcgaggag
      961 ctatggaagg cgaagcccgg agagacctac cgggacatct tcgagcgggt ggagcctcag
     1021 gtgatgtccg tgttccgcag ccagcagccg cagctcgaag gggtctggga ccgcgaggtc
     1081 ttcggcctgc ggcacttcga gccgctccgc ttcgtgccgg tcctcggccg gcaccgggac
     1141 ggccgggtcc tcctgggggc cggcgcgtcc agcggcatca cgataggctc gcggtgggag
     1201 atccaccccc cgaaagctca ggacgtggtc ccgggcaccg agctcgggct ggtggagatc
     1261 accggcgtct gtgccttcac cagcgagggc cggatcgtgc gcgaggcccg gcccgacttc
     1321 gtgggccggg agtgccgcgc cgtggagcgt gcccacagcc atggcgatct gggatgggcc
     1381 gtggacctgg tcatcccgca cgcctggaag gagcacatcg ggatcctgga ggacgggatc
     1441 cgggagtcgc ccctcctgcg cccagcctcc ggcgaggagg ctcacacccg cgcctacctc
     1501 ctcgggcccc gccgggctgc cggtccgggc gaccccgctc ccatgctcga gaccctcgag
     1561 gagccctgct gggtagccgt gggcaaggat ggccgcctgg tgctgccggt gcgccgggcc
     1621 acggcgcccg agtcggcgtt cgacctgctg gagaatctgg agaagctggc ccgcttccat
     1681 cacgcgctag ccttcgacaa ccctaccagc ccgcttcgtg gcaaggtgga cctggtcgtg
     1741 aagcgcctga cggacggcgg cccggtaccg gcacggcagg acgacggcca gaccgccttc
     1801 tacgaaggcg agctcctggc tctggaagcg atcaacagga cgggccagaa actccacctc
     1861 tacgccatcg acttcggcct gtccggcaag gtgagcctgc tctatccccc ctacggaacc
     1921 cgggaggcga cctcgcggga gacgctctcc atcaacaccc gcctcggtca ggagtggcag
     1981 ctcacggtcc ctgacgcctt cccgttcgag gcgcccggca cggccgcttc aggggtggag
     2041 ttcgtcaagc tcttcgccac caccgagccg gtagacttca agctcctctt ccaggagagc
     2101 tttcgccgcg acggggattt cctgcccggc atcgacgacc atccgctcgg ccgtctcctc
     2161 agcgaggccg tctgggggat cacctaccgc ggcccgcttc ccgggggagc ggcaaggacc
     2221 gccgcggact ggacaacggc cacccgaagc ttcgtgctcc gccgccgcac ccgggcctgg
     2281 tgagctgctt ccccgcggct cgggtgcggc aggcgggtgg gacgagccga agctccgtca
     2341 ttccctcggg aatgatcgcc gttcccctgg aaatcatcgc cattcccgtg ggaacgacga
     2401 gcgttccttc aggaatgaag gccgttcccg cggaatggag gctgttcctt caggaaggaa
     2461 ggccgtttcg gggggaatgg ggaccgtttc ttcagacatg gcggccattc cttcaggaat
     2521 gaaggcgatt ccccgaagga tggtgctcat tcccgaagga ttcgtggccg tttccggagg
     2581 aagagagccc tttttgagtt gatcgttgcc gaagccgtga gtgggggaag tgggagagag
     2641 ggcccggtga ggcctccctg ggcagatcgc gcgccgcggc ctccgcctcg agatctaggt
     2701 acggcgaatg gggcgtcatg gtatattcgt cgatctcact ccagccacgt tggagaagca
     2761 ccgatgatcg gcgagatcta tcaagatttg cgtttttgct tcagatccct tttccgccgt
     2821 ccgtccgccg cgatcgtcgc cgcggtcgtc gcgatggcga tcgggatcgg cgccgcggtg
     2881 gccatcttca gtgtcgtcaa gacggtgctg ctccaaccgc tgccctatcc gaacgcggat
     2941 cgggcagtaa ccgtctggaa tcagttcatc cagctcgacc ttccccaggt gcagctttcc
     3001 gagcccgagc tctatgagta cgtggagcgg agccagtcac tccaggcgct ggccgcgttc
     3061 gtggtcggcc aaggcaatct caccggagtc gacgacccgg agcgggtcga ctatgcggcg
     3121 gtgtccccgc aactgttctc catcctgggg gtcaaggcca gccagggccg cgtcttcgtc
     3181 gagggcgagg accggccgga cgcgccgcgc atcatcgtgc tcagtgacgc gttctggcgc
     3241 cggcggcagg gggcggatcc gaacgcggtc ggccgtgaca tgtacgtcaa cggcaaccgt
     3301 tacacgatca tcggcatcat gccccccggc ttccgttttc cggagaagat cgacctctgg
     3361 ttgccgttgc gtctggatcc ggcgaatccc ggaatgcgcg gcaaccacta tctggaagct
     3421 ctcgggcagt tgaagcccgg cgtcaccctc agccaggcgc aggccgagct ggacgtcatc
     3481 gcccagcaac tgcagaaaga gtttccgatg tcctacccgg ccgacagcgg ctggggggtc
     3541 aacctgatcc cgctctatga agacatgacc ggggaggtcc gtccggctct cctcatcttc
     3601 ctcgcggcgg tcgggctcgt cct
//
LOCUS       DASPJI010000293         4925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_311822, whole genome shotgun sequence.
ACCESSION   DASPJI010000293 DASPJI010000000
VERSION     DASPJI010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311822"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /gene="hslU"
                     /locus_tag="VEL74_07710"
     CDS             complement(<1..1363)
                     /gene="hslU"
                     /locus_tag="VEL74_07710"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HYG62452.1"
                     /translation="MSIQLSGEVQSVPAIENMTPRQIVAELDKYVVGQKQAKRAVAIA
                     LRNRWRRQQLPPELGEEIAPKNILMIGPTGVGKTEIARRLARLAKAPFLKVEASKFTE
                     VGYVGRDVESMVRDLVEIAVTMVKEEKREAVREAAALRAEDRLLQLLVPPRPISSYLR
                     HEPEEDDDLAATREKFREKLRAGALDHRRVEIEVDEASYPSLEMFTPQGVEEVGLNIK
                     DMLPGLFGRKKKQQVTVAEAREILLQQESEKLIDRAQVAREARERVEQGGMIFLDEID
                     KIAGREGSRGPDVSREGVQRDLLPIVEGTNVSSKYGMVKTDHILFIAAGAFHVSKPSD
                     LIPELQGRFPIRVELDALTEQDFVRILTEPETALTKQYSALLSTEGVALDFTADAVAE
                     LARLAVEVNSATENIGARRLSTLMERLLDEVSFEAPEMAGASVNVDADYVRRVLSGIV
                     KDQD"
     gene            complement(1368..1910)
                     /gene="hslV"
                     /locus_tag="VEL74_07715"
     CDS             complement(1368..1910)
                     /gene="hslV"
                     /locus_tag="VEL74_07715"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HYG62453.1"
                     /translation="MDTFASTTVLLVRREGKVCMASDGQVTMNQSTIVKAGARKVRRV
                     GKGKVLVGFAGGGADGLALVTRLEEKLEEFNGQLERAVVELAKDWRTDRALRQLQAVM
                     VVTDKDKSFFVTGSGELLQPDDEDGILAVGSGSNFALAAARALMRNTSMSAADIVREA
                     MRIAAEICVFTNDRLTLEEL"
     gene            complement(2013..2951)
                     /gene="xerC"
                     /locus_tag="VEL74_07720"
     CDS             complement(2013..2951)
                     /gene="xerC"
                     /locus_tag="VEL74_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HYG62454.1"
                     /translation="MRQLIHRYLEHIQAERNLSPHTVAAYESDLTRFHHFLAAEFLGC
                     DPGEVRPQDVDVLAVRSFLAWLTREGIGKRSQGRALSAVRSLFRFACREGTLSANPAQ
                     GVRTPKFPKTLPRHLRPGEIENLVEAPAAGDGPLALRDRAILELLYATGLRVSELVGL
                     DWRDIDLSARVLRTMGKGSKERMVPFGRPAADALRTWLGVWEGVRGPKASEDEEPVFL
                     NHVGGRLTRRSVERVIDKWVEQAALAKGVHPHTLRHTFATHLLENGADLRSIQEMLGH
                     SSLSTTQKYTHLEVERLLAVYRDAHPRARTPGTPGK"
     gene            complement(2952..4316)
                     /gene="trmFO"
                     /locus_tag="VEL74_07725"
     CDS             complement(2952..4316)
                     /gene="trmFO"
                     /locus_tag="VEL74_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HYG62455.1"
                     /translation="MSSPIHIVGGGLAGTECAWQLAERGIPVVLHEMRPVQPTPAHKT
                     GNLAELVCSNSLRSDDPLHAAGLLKREMESFGSLIIGAARAAAVPAGSALAVDRDRFA
                     ERVTETLARHPRIETRREEVTEIPEGEVVLGTGPLTSPAMSARLQELLGSEYLYFYDA
                     IAPIVEADSLDMSKLFWQSRYGKGEGEDYLNAPMDREQYAAFYEALLAAEVLVPHDFE
                     KAIFFEGCLPIEELARRGVDTLRYGPMKPVGLTAPDGRRPWAVVQLRQENLAKSQLNL
                     VGFQSRMKWGDQQRVLSMIPGLEQARFVRFGQIHRNTFINAPTHLDKLYRVKAAPRVR
                     LAGQLTGVEGYLESAATGLATGLYLALERRDGRAPEPFPPTTALGALARHLTESDPKH
                     FQPANINYGLFTELDTRVRKGDKRAALADRARTELALWARRHQLAQMPAEEPETAEAA
                     VPGV"
     gene            complement(4334..4651)
                     /gene="sugE"
                     /locus_tag="VEL74_07730"
     CDS             complement(4334..4651)
                     /gene="sugE"
                     /locus_tag="VEL74_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HYG62456.1"
                     /translation="MAWVYLIIAGLLEIVWAVSLKRTEGFTKLGPSLFTLVTMAASFY
                     LLSLALRTLPTGTGYAVWVGIGAAGTALLGILFLGEPRTAFRLISLALILLGVIGLRL
                     AEG"
     gene            complement(4673..>4925)
                     /locus_tag="VEL74_07735"
     CDS             complement(4673..>4925)
                     /locus_tag="VEL74_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62457.1"
                     /translation="DDQTRRFLTGPLGVRMKEDGGELELQVTKLMDWFAEDFERWGGG
                     RVAFLRKYLPPAQQKRIAAAKEIDLEFRDYSWKLNDASR"
BASE COUNT          677 a         1678 c         1790 g          780 t
ORIGIN      
        1 ggtcctgatc cttgacgatg ccggagagga cacggcggac gtagtcggcg tccacgttga
       61 cgctcgcccc cgccatttcg ggagcctcga acgacacctc gtcgagcagg cgctccatga
      121 gcgtggagag ccggcgggcg ccgatgttct cggtggcgct gttgacctcg accgccaggc
      181 gggcgagctc ggccaccgcg tcggcggtaa agtcgagcgc cacgccctcg gtcgagagca
      241 gggcggagta ctgcttggtg agcgcggtct cgggctcggt caggatgcgc acgaagtcct
      301 gctcggtcag cgcgtcgagc tccacccgga tggggaagcg gccctgcagc tcggggatca
      361 ggtccgaggg cttgctgacg tggaacgcgc cggcggcgat gaacaggatg tggtcggtct
      421 tgaccatgcc gtacttgctg gagacgttgg tcccctcgac gatgggcagc aggtcgcgct
      481 gcacgccctc gcgcgacacg tcggggccgc ggctcccttc gcggccggcg atcttgtcga
      541 tctcgtcgag gaagatcatg cccccctgct cgacgcgctc gcgggcctcg cgggccacct
      601 gggcgcggtc gatcagcttc tcggactcct gctgcaggag gatctcgcgc gcctcggcga
      661 cggtgacctg ctgcttcttc ttgcggccga agaggccggg gagcatgtcc ttgatgttga
      721 gccccacctc ttcgacgccc tgcggggtga acatctcgag gctcgggtag gaggcctcgt
      781 cgacctcgat ctcgacgcgc cggtggtcga gagcgccggc gcgcagcttc tcgcggaact
      841 tctcgcgcgt ggcggcgagg tcgtcgtcct cctcgggctc gtggcgcagg tagctgctga
      901 tcgggcgcgg cggcaccagg agctggagga ggcggtcctc ggcgcggagg gccgcggctt
      961 cgcgcaccgc ctcgcgcttc tcctccttga ccatggtcac cgcgatctcc accaggtcgc
     1021 gcaccatcga ctcgacgtcg cggccgacgt agcccacctc ggtgaacttg ctggcctcga
     1081 ccttgaggaa gggggccttg gcgaggcggg cgaggcggcg ggcgatctcg gtcttgccga
     1141 cgccggtggg gccgatcatc aggatattct tgggcgcgat ctcctcgccc agctcgggcg
     1201 ggagctgctg gcgccgccag cggttgcgca gggcgatggc caccgcgcgc ttggcctgct
     1261 tctgcccgac cacgtacttg tcgagctccg cgacgatctg ccgcggagtc atattctcga
     1321 tcgccgggac gctctgcact tcgccggaga gctggataga catcgggtca gagttcctca
     1381 agggtcaggc ggtcgttggt gaagacgcag atctcggccg cgatcctcat cgcttcccgc
     1441 acgatgtcgg cggccgacat cgaggtgttg cgcatcagcg cccgcgcggc ggccagggcg
     1501 aagttcgagc cggagcccac ggcgaggatg ccgtcctcgt cgtcgggctg caacagctcg
     1561 ccggagccgg tgacgaagaa gctcttgtcc ttgtcggtga ccaccatcac cgcctgcagc
     1621 tggcgcaggg cgcggtcggt ccgccagtcc ttggcgagct cgacgacggc ccgctcgagc
     1681 tggccgttga actcctcgag cttctcttcc agacgggtga cgagcgccag gccatcggcg
     1741 ccgccgccgg cgaagccgac cagcaccttg cccttgccga cgcgccgcac cttgcgcgcc
     1801 ccggccttga cgatggtgct ctggttcatc gtcacctggc cgtcgctcgc catgcagact
     1861 ttgccttcac ggcgtacgag gaggacggtc gtcgaggcga atgtatccat aggcgtgtgg
     1921 attgtaacag ggccattcac cggggagggg gactcctcct ccggctttgc cgcatcccgg
     1981 cgaagcagcc tatcagagtc ccggcccggg ggtcacttcc ccggtgtccc cggggtccgc
     2041 gcccgggggt gggcgtcgcg gtagaccgcc agcagccgct cgacctccag gtgggtgtac
     2101 ttctgggtgg tcgacagcga gctgtgcccc agcatctcct ggatcgaccg caggtcggcg
     2161 ccgttctcca acagatgggt ggcgaaggtg tggcgcaggg tgtgggggtg gacccccttg
     2221 gcgagggcgg cctgctcgac ccacttgtcg atcacccgct cgacggagcg gcgggtcagc
     2281 cggccgccca cgtggttgag gaagaccggc tcctcatcct cgctcgcctt gggaccgcgc
     2341 accccttccc agacgccgag ccaggtccgc agggcgtcgg ccgccggccg gccgaagggg
     2401 accatgcgct ccttgctccc cttgcccatg gtgcggagca cgcgggcgga gaggtcgatg
     2461 tcgcgccagt cgagccccac gagctcgctg acgcgcaggc cggtggcgta gagcagctcg
     2521 aggatcgccc ggtcgcgcag ggcgagcggc ccgtcgccgg cggccggggc ctcgaccagg
     2581 ttctcgatct cgcccggccg caggtggcgg gggagggtct tggggaactt gggggtccgc
     2641 accccctgcg ccgggttggc ggaaagcgtc ccctcgcggc aggcgaagcg gaacaggctg
     2701 cgcaccgccg acagcgcccg cccctggctg cgcttgccga tcccctcgcg ggtgagccag
     2761 gcgaggaagg agcgcaccgc cagcacgtcg acatcctgag gccgcacctc cccggggtcg
     2821 cagccgagga actccgcggc gaggaagtgg tggaagcggg tgaggtccga ctcgtaggcc
     2881 gccacggtgt ggggcgacag gttgcgctcg gcctggatgt gctcgaggta ccggtggatg
     2941 agctgtcgca ttcagacgcc cggcacggcg gcctcggccg tctccggctc ctcggcgggc
     3001 atctgggcga gttggtggcg ccgggcccag agcgcgagct cggtccgcgc gcgatcggcg
     3061 agggcggcgc gcttgtcccc cttgcggacg cgggtgtcga gctcggtgaa caggccgtag
     3121 ttgatgttgg ccggctggaa gtgcttgggg tccgattccg tgaggtgccg ggccagggcc
     3181 cccagcgcgg tggtgggggg gaacggctcg ggcgcgcggc cgtcccggcg ctcgagggcg
     3241 aggtacagcc cggtggcgag gccggtggcg gcggactcca ggtacccctc gacgccggtg
     3301 agctggccgg cgaggcgcac ccgcggcgcg gccttcaccc ggtagagctt gtcgaggtgg
     3361 gtcggcgcgt tgatgaaggt gttgcggtgg atctgcccga aacggacgaa gcgcgcctgc
     3421 tcgagccccg ggatcatgga caggacgcgc tgctgatccc cccacttcat gcgcgactgg
     3481 aaccccacga ggttgagctg gctcttcgcc aggttctcct gccggagctg gaccacggcc
     3541 caggggcgcc ggccgtcggg cgcggtgagc cccaccggct tcatcggccc gtagcgcagg
     3601 gtgtccacgc cgcggcgggc cagctcctcg atcggcaggc acccctcgaa gaagatcgcc
     3661 ttctcgaaat cgtgcgggac gaggacctcg gcggcgagga gggcctcgta gaaggcggcg
     3721 tactgctccc tgtccatcgg ggcgttgaga tagtcctccc cctccccctt gccgtagcgc
     3781 gactgccaga acagcttgga catgtcgagg ctgtccgcct cgacgatggg ggcgatggcg
     3841 tcatagaagt agaggtactc gctccccagc agctcctgga gccgggcgga catggccggc
     3901 gaggtgagcg gcccggtgcc gaggacgacc tccccctcgg ggatctcggt cacctcctcg
     3961 cgccgcgtct cgatccgggg gtggcgcgcc agggtctccg tcacccgctc ggcgaagcgg
     4021 tcgcggtcga cggccagggc gctgccggcc ggcaccgccg ccgcccgcgc cgcgccgatg
     4081 atcagcgagc cgaaggactc catctcgcgc ttgaggagcc cggccgcgtg gagcgggtcg
     4141 tcgctgcgca acgagttgct gcacaccagc tccgccaggt tccccgtctt atgggccggc
     4201 gtcggctgga ccggccgcat ctcgtgcagc acgacgggga tcccgcgctc ggcgagctgc
     4261 caggcgcact cggtccccgc gaggccgccg ccgacgatat ggatgggaga ggacatcctt
     4321 atctatccct ccatcatcct tcagccagcc ggagcccgat gactcccagg aggatcaggg
     4381 cgagcgagat cagccggaac gccgtccgcg gctctcccag gaagaggatg ccgaggagag
     4441 ccgtgccggc ggcgccgatc cccacccaga cggcgtagcc ggtgccggtg gggagggtgc
     4501 gcagggcgag gctcagcagg tagaagctcg cggccatggt caccagggtg aagaggctcg
     4561 gccccagctt ggtgaacccc tccgtccgct tgaggctgac cgcccagacg atctccagca
     4621 gtccggcgat gatgaggtag acccaggcca tggggtggat tataggggcg cctcaccgcg
     4681 acgcgtcgtt cagcttccag ctatagtccc ggaactccag gtcgatctcc ttcgccgcgg
     4741 cgatgcgctt ctgctgggcc ggaggcaggt acttgcgcag gaaggccacc cggccgccgc
     4801 cccagcgctc gaagtcctcg gcgaaccagt ccatcagctt ggtgacctgc agctccagct
     4861 ccccgccgtc ctctttcatg cggacgccca gcgggccggt caggaagcgg cgcgtctggt
     4921 cgtcg
//
LOCUS       DASPJI010000294         6884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_704536, whole genome shotgun sequence.
ACCESSION   DASPJI010000294 DASPJI010000000
VERSION     DASPJI010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6884)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704536"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VEL74_07740"
     CDS             <1..269
                     /locus_tag="VEL74_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62458.1"
                     /translation="GAGRVVPTASDDSGLFWFFAPENWEMLVKVLDGCSLNGHYWVFA
                     AATTTVQYDLTVTDTRTGQVARYHNAQGTAAPATTDTGALEVCP"
     gene            491..2095
                     /locus_tag="VEL74_07745"
     CDS             491..2095
                     /locus_tag="VEL74_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HYG62459.1"
                     /translation="MTHDLDREIFADFLLESQERLARLEEVLLALSEGAGDAVPQLLD
                     EARLQLHTLKGNSGMMGLAELQAVAHALEDRVDSLDHDAPQVEDLLHGVDRFRELLEE
                     VARLQEPAAAAADRTDAAGAAGAAGAIGGVAAMELGVNTDGVRVSFATLDRLVDLLGE
                     MVIFRNRMSDAVEQQRGAAASGRGWEHVQAAHERLGKTLDALRDGVMRLRMVPLRTLF
                     GHLGRIVHDESLREGKSVRFETRGGETPLDRALLELSSEALGHLVRNCVVHGLETTAE
                     RVRAGKPAQGTVRLAAEANTREVVIDVVDDGGGIDRSRVTATARRLGHVLGEGEDPLS
                     LLFLPGFSTRDGADMSSGRGIGLAAVQKAVQRRGGRIEVFSEEGFGTLFRLRLPLSVS
                     ITRALLLASDGEEYALPLAAIVESVQIDGLELHEMNGATVLPWRGGLIPLLDLGHSFG
                     TSPVRRHSGFAVIIEAAGSQRALAVERIHGIREVVVKGLDRVAGSPPGISGSTILGDG
                     RAVLILDPAGLMNLSPFAGATAGGKA"
     gene            2092..2850
                     /locus_tag="VEL74_07750"
     CDS             2092..2850
                     /locus_tag="VEL74_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HYG62460.1"
                     /translation="MKKKRGSHEPGDGATFRLPESGLAEDILKAFASSMAAEEAEEAE
                     EDDGTAGGPAEAPAPAREPGRKPSSSAQGTDRVYAFVDRLERHAAREADEAREDPETW
                     VTFDLSGEVYGLPVARIEEVLRVTAITRVPHAPTPVRGITNMRGRVLAVVDLRVRLGL
                     PPAPIDAQSRILVVSSRERSIGLLADAARQVIKILPSSVQPPPTDVVTSRSDFIVGVV
                     RAGETLVILLDIDRVLLIQEGTEMPEPEHEEAAL"
     gene            2847..3323
                     /locus_tag="VEL74_07755"
     CDS             2847..3323
                     /locus_tag="VEL74_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62461.1"
                     /translation="MSRLAKKVARRMAGALLALAAVAAVLVPVAARRAAASEFYVVIV
                     NQENPLSVLPASEVSRIFLKRSERWPNGELILPIDLPEGSPVRESFSKEVHDRSTAAI
                     RAYWQQLIFSGRGLPPPEKASGADIVEYVRSHRGGIGYVSVRTSLGAGVKVLKVTD"
     gene            3346..5304
                     /locus_tag="VEL74_07760"
     CDS             3346..5304
                     /locus_tag="VEL74_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HYG62462.1"
                     /translation="MFDRLKFAHKVALMPGLAAIGFLIILAVSLIVGAGNGRRLEDIQ
                     LRYFPALEASQQLGDKLTELQRTLQDAVAAQDTVKVTEEAETLKNEFNALKAKASSNT
                     AIRSELINKIGRDFDQYYALARKTTLDMISGQAGGQTAAALPRMTSQYNQLLTDLGEL
                     TESAKTAMNEAFIQAENRQQWSLWTIVLVTVVSVLGLALLSYFVIRSLTAPLSASVEV
                     ARRLARGDMSGRVAARSNDEVGELASAMEQMIAYLRDMARVADSIAEGDLRVDVEPRS
                     EADIFGSAFRRMTANLRQMIGDVKQSASQVASTADEISASSLQIKRGAESQSSSTEET
                     SATMVEMAAQIDSINRSTQMLASHVQETSSSIEEMAASIEEVAHSSEDLLSSVGETSS
                     TIEEMTASTRSVATRVQVVDEVSKSAADAAGEGGDRLSRVIMGIGNSTKDIGKIVKTI
                     GEFADQTNLLALNAAIEAARAGDAGRGFAVVAEEVKRLAERSMHSTREITNFVDAVQR
                     DTEEAVSLSQQVLQQIVGAVNRTTDLVRDVHSATQEQSAGAAQILRTSSGMQEVTQQL
                     AAAAREQANGARQIMGSVEAMNRMTQQVADATNEQKRGGDQVVKAVDQIAQVAQQYLS
                     ATEQLSNATHSLAVEAERLKKLAGVFQV"
     gene            5348..>6884
                     /locus_tag="VEL74_07765"
     CDS             5348..>6884
                     /locus_tag="VEL74_07765"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HYG62463.1"
                     /translation="MASIEPIKSPPTWEECMELLDRLPSLPLDERMEAIERLVRNSSP
                     GIRERALRVGAAVLSDDKLTEYLRNDEDAVLRNAGLEIFKMRGGRSFPLALRLLKDPD
                     DDVALQAVLILDHLRDPRALEPLRTVLDHNDTNVTQAALLAMGKLGDARCIPDLLPFL
                     EGDPWLQMASVQALGDLRSPLAVKPLSGLLTDLLVGSLAAEAMARVGGPAAFRALAAH
                     WLAYREQLETESMLGLLAHVLEGLSQAPSAEEGLVESLAAHLGDPSPDARSAAARCLL
                     SLGPTAWDERALDALTAAQPPGGGSLPACLTRRPDLIAALLARTGELRAWGFELAARH
                     PRAIPAEAFFATLPEAAEAPDLLDPIVQALARFRHPELGGALLSLYLRLPAEARGALA
                     PVIKSHARELRQALDERRDVDAADRLVLAARLGKPAREVARELQELPPAGRPAAVAQL
                     EELKPVLRLLPWEQWIEEAPDVYTDLAAEAASRAELRELLPALRARAGTTSTPALIRA
                     FGSL"
BASE COUNT          994 a         2542 c         2419 g          929 t
ORIGIN      
        1 ccggcgccgg ccgcgtcgtc cccaccgcct ccgacgactc ggggctcttc tggttctttg
       61 cccccgagaa ctgggagatg ctggtcaagg tgctggacgg ctgctccctc aacggccact
      121 actgggtctt cgccgccgcc accacgaccg tgcagtacga cctgaccgtc accgacaccc
      181 gcacggggca ggtggcgcgc taccacaacg cgcagggcac cgccgccccc gccaccaccg
      241 acaccggcgc cctcgaagtc tgtccatagt cgaagcaagt cggcaaccgg ttatgcgcga
      301 ctgttcaagt gcaccggttt cgatgtagac tccccccgtt ctttcgcgga acagcctgag
      361 gaacgcaggc tcgcctcgac cggagagagg cccgccacgg cggtgtcttc tcgacggaga
      421 gcggcgagag caccgcctgc gtcccgtgac cgcgcctggc acccgactgg tacccgagct
      481 cgacgctgcg atgacgcacg acctggaccg agagatcttc gccgacttcc tcctggagtc
      541 ccaggagagg cttgcccgtt tggaggaggt gctgctcgcc ctgtccgaag gggcgggcga
      601 cgccgtcccc cagctcctgg acgaggcccg gctgcagctc cacaccctca agggcaactc
      661 cgggatgatg gggctcgccg agctccaggc ggtcgcccac gccctggagg accgggtgga
      721 ctcgctcgac cacgacgctc cccaggtcga ggacctgctg cacggcgtcg accgcttccg
      781 cgagctgctg gaggaggtgg cgcgcctgca ggagccggcg gccgcggccg ccgaccggac
      841 cgacgcggcc ggcgcggcgg gagccgccgg cgctatcggc ggggtggcgg ccatggagct
      901 cggcgtcaac accgacggcg tgcgggtctc cttcgccacc ctggaccggc tggtcgacct
      961 gctgggcgag atggtgatct tccgcaaccg gatgagcgac gcggtggagc agcagcgcgg
     1021 cgccgcggct tccgggcgcg gctgggagca cgtccaggcc gcccacgagc ggctcggcaa
     1081 gaccctggac gccctgcgcg acggcgtgat gcgcctgcgc atggtgccgc tgcgcaccct
     1141 cttcggccac ctcggccgca tcgtccacga cgagagcctg cgcgagggca agtcggtccg
     1201 cttcgagacg cggggcggcg agacccccct cgaccgggcg ctcctcgagc tgtcgagcga
     1261 ggccctgggg cacctcgtgc gcaactgcgt ggtgcacggc ctcgagacca cggcggagcg
     1321 ggtgcgggcg ggcaagcccg cccagggcac ggtgcgcctc gccgccgagg ccaacacccg
     1381 cgaggtggtc atcgacgtgg tggacgacgg cggcggcatc gaccgctcgc gggtgaccgc
     1441 caccgcccgg cggctggggc acgtgctcgg cgaaggggag gacccgctgt cgctgctctt
     1501 cctgcccggc ttctcgacgc gcgacggggc ggacatgagc tccgggcgcg gcatcggcct
     1561 cgccgccgtc cagaaggcgg tgcagcggcg cggcggccgc atcgaggtct tctccgaaga
     1621 ggggttcggc accctgttcc ggctccgcct gccgctcagc gtctcgatca cccgcgccct
     1681 gctgctggcg agcgacggcg aggagtacgc cctgccgctc gccgccatcg tcgagagcgt
     1741 gcagatcgac ggcctggagc tgcacgagat gaacggcgcc acggtgctgc cctggcgcgg
     1801 cggcctgatc cccctgctcg acctcggcca cagcttcggc acctcgccgg tccgccgcca
     1861 ctccggcttc gcggtgatca tcgaggccgc cggcagccag cgggccctgg cggtcgagcg
     1921 catccacggc atccgcgagg tggtggtcaa ggggctcgac cgggtggccg gctcgcctcc
     1981 cggcatctcg ggctccacca tcctgggcga cggccgcgcc gtgctcatcc tcgatcccgc
     2041 gggcctgatg aacctgtcgc cgttcgccgg cgcgacggct ggaggaaaag cttgaagaag
     2101 aagcgtggaa gccacgaacc cggggacggc gcgacgttcc ggctgccgga gtccggcctg
     2161 gccgaggaca tcctcaaggc gttcgcctcc tccatggccg ccgaggaggc cgaggaggcc
     2221 gaggaggatg acgggacggc cggcgggccg gcggaggccc cggccccggc gcgggagccg
     2281 ggccgcaagc cgtcctcctc ggcccagggg accgaccggg tctatgcctt cgtcgaccgc
     2341 ctcgagcgcc acgccgcgcg cgaggccgac gaggcccgcg aggaccccga gacctgggtg
     2401 acgttcgacc tctccggcga ggtctacggc ctgccggtgg cgcgcatcga ggaggtgctg
     2461 cgggtgaccg ccatcacccg cgtgccgcac gcgccgacgc cggtgcgcgg catcaccaac
     2521 atgcgcggcc gggtgctggc ggtggtcgac ctgcgggtgc ggctcggcct gccgccggct
     2581 ccgatcgacg cgcagagccg gatcctcgtc gtctcgtcgc gggagcgctc gatcggcctg
     2641 ctggccgacg ccgcccggca ggtgatcaag atcctgccca gcagcgtcca gccgccgccg
     2701 acggacgtgg tgacctcacg ctctgatttc atcgtcggcg tcgtccgcgc cggcgagacc
     2761 ctggtcatcc tgctggatat cgaccgggtg ctgctcatcc aggaggggac cgagatgccc
     2821 gagcccgagc acgaggaggc ggcgctgtga gccgtctggc aaagaaggtg gcacggcgga
     2881 tggccggggc gctcctcgcc ttggccgccg tcgccgccgt cctcgtcccg gtggccgcgc
     2941 ggcgcgccgc cgcgtccgag ttctacgtcg tgatcgtcaa ccaggagaac cccctgtcgg
     3001 tcctgccggc gtcggaggtc tcccggatct tcctcaagcg gtcggagcgc tggcccaacg
     3061 gcgagctgat cctgcccatc gacctgccgg agggctcgcc ggtgcgggag agcttctcga
     3121 aagaggtcca cgaccggagc acggcggcga tccgcgccta ctggcagcaa ctgatcttct
     3181 ccggccgcgg tctgccgccg ccggagaagg cctcgggagc cgacatcgtg gagtacgtgc
     3241 gctcccatcg ggggggcatc ggctatgtct cggtccgcac ctcgctgggc gccggggtca
     3301 aggtcctcaa ggtgacggat tgatggggtt tcgaggggag agtccatgtt cgatcgattg
     3361 aagttcgcgc acaaagttgc cctgatgccg ggcctcgcgg ccatcggctt cctgatcatc
     3421 ctggcggtct cgctgatcgt cggagcgggc aacgggcggc gtctcgagga tatccagctg
     3481 cgctatttcc ccgctctgga ggccagccag caactgggcg acaagctgac ggagctccag
     3541 cgcacgctgc aggacgccgt cgccgcccag gatacggtca aggtcacgga agaggccgag
     3601 accctcaaga acgagttcaa cgcgctcaag gccaaggcga gcagcaacac cgccatccgc
     3661 agcgagctga tcaacaagat cgggcgcgac ttcgaccagt actacgccct cgcccgcaag
     3721 accaccctcg acatgatctc gggccaggcc ggcggccaga ccgccgccgc cctgccgcgg
     3781 atgaccagcc agtacaacca gctcctcacc gacctggggg agctgaccga gagcgccaag
     3841 accgccatga acgaggcctt cattcaggcc gagaaccggc agcagtggtc gctctggacc
     3901 atcgtcctcg tcaccgtggt gagcgtcctc ggcctcgccc tgctgtccta cttcgtcatc
     3961 cgctcgctga ccgcgccgct gtccgcctcg gtcgaggtgg cgcggcgcct ggcccgcggc
     4021 gacatgtcgg gccgggtggc ggcgcgctcc aatgacgagg tgggcgagct cgccagcgcc
     4081 atggagcaga tgatcgccta cctgcgcgac atggcgcggg tggccgactc catcgccgag
     4141 ggggacctgc gtgtcgatgt cgagccgcgc tccgaggcgg acatcttcgg cagcgccttc
     4201 cgccgcatga cggccaacct ccggcagatg atcggcgacg tcaagcagtc ggcgtcgcag
     4261 gtcgcctcca ccgccgacga gatctccgcc tcgtcattgc agatcaagcg cggcgccgag
     4321 agccagtcct cgtccaccga ggagacgtcg gcgaccatgg tcgagatggc ggcgcagatc
     4381 gacagcatca accgctcgac ccagatgctc gcctcgcacg tgcaggagac ctccagctcc
     4441 atcgaggaga tggccgcctc catcgaggag gtggcgcaca gctcggagga cctcctgtcg
     4501 tcggtgggcg agacctcctc gacgatcgag gagatgaccg cctcgacccg ctcggtggcg
     4561 acccgcgtcc aagtggtgga cgaggtgtcg aagagcgccg ccgacgccgc gggcgagggg
     4621 ggcgaccggc tctcccgtgt catcatgggc atcggcaact cgaccaagga catcggcaag
     4681 atcgtcaaga ccatcggcga gttcgccgac cagaccaacc tgctggccct gaacgccgcc
     4741 atcgaggcgg cgcgggcggg cgacgcgggg cgcggcttcg ccgtcgtcgc cgaggaggtc
     4801 aagcggctgg ccgagcgctc catgcactcc acccgcgaga tcaccaactt cgtcgacgcg
     4861 gtccagcgcg acaccgagga ggccgtctcc ctgtcgcagc aggtgctgca gcagatcgtc
     4921 ggcgcggtca accgcaccac cgacctggtg cgcgacgtcc actcggccac ccaggagcag
     4981 agcgccggcg cggcgcagat cctccgcacc tcgagcggca tgcaggaggt cacgcagcag
     5041 ctcgcggcgg ccgcccgcga gcaggccaac ggcgcgcgcc agatcatggg ctccgtcgag
     5101 gcgatgaacc gcatgaccca gcaggtggcc gacgccacca acgagcagaa gcgtggcggc
     5161 gaccaggtgg tcaaggccgt cgatcagatc gcccaggtcg cgcagcagta cctgtccgcg
     5221 accgagcagc tctccaacgc cacccacagc ctcgccgtcg aggccgagcg gctgaagaag
     5281 ctcgccggcg tcttccaggt ttgagaggga ccgcggacgc accggttatc acctaggagt
     5341 ttcaaccatg gccagcatcg aaccgatcaa gtcgccgccc acctgggaag agtgcatgga
     5401 gctcctcgac cggctcccgt cgctgccgct cgatgagcgc atggaagcca tcgagcggct
     5461 ggtgcgcaac tccagccccg gcatccgcga gcgggcgctg cgcgtcggcg ccgccgtcct
     5521 ctccgacgac aagctgacgg agtacctgcg gaacgacgag gacgccgtgc tgcgcaacgc
     5581 cggcctcgag atcttcaaga tgcgcggcgg ccgcagcttc cccctcgccc tgcgcctgct
     5641 caaggacccg gacgacgacg tggcgctcca ggcggtcctg atcctcgacc acctgcgcga
     5701 cccgcgggcg ctggagccgc tgcgcaccgt gctggaccac aacgacacca acgtcaccca
     5761 ggcggcgctg ctggccatgg gcaagctcgg cgacgcgcgc tgcatccccg acctgctgcc
     5821 gttcctcgaa ggggacccgt ggctgcagat ggcctcggtg caggcgctgg gcgacctgcg
     5881 ctcgccgctg gcggtcaagc cgctgtccgg cctgctgacc gacctgctgg tgggatcgct
     5941 cgccgccgag gcgatggcgc gcgtcggcgg ccccgccgcc ttccgggcgc tcgcggcgca
     6001 ctggctcgcc taccgcgagc agctcgagac cgagagcatg ctgggccttc tggcgcacgt
     6061 gctggagggg ctctcccagg ctccctcggc ggaggagggg ctggtcgaat cgctcgccgc
     6121 ccacctcggc gacccgtctc ccgacgcgcg ctcggccgcc gcccgctgcc tcctctcgct
     6181 cggcccgacg gcctgggacg agcgggccct cgacgccctg accgcggcgc agccgccggg
     6241 cggcggcagc ctgccggcct gcctcacccg gcgcccggac ctgatcgcgg ccctgctcgc
     6301 gcgcaccggt gagctgcggg cctggggctt cgagctggcg gcgcgccatc cgcgggcgat
     6361 cccggccgag gccttcttcg ccaccctgcc ggaggcggcc gaggcgccgg acctgctgga
     6421 ccccatcgtc caggcgctcg cccgcttccg ccatccggag ctgggcgggg cgctgctctc
     6481 cctctatctc cgtctaccgg cggaggcccg gggggcgctg gcgccggtga tcaagagcca
     6541 tgcgcgggag ctgcgccagg ccctcgacga gcgccgcgac gtcgacgccg ccgaccggct
     6601 ggtgctcgcg gcgcgcctcg gcaagccggc gcgggaggtg gcgcgggagc tccaggagct
     6661 gccgccggcg ggccgtcccg ccgccgtcgc ccagctcgag gagctcaagc cggtgctgcg
     6721 cctgctcccc tgggagcagt ggatcgaaga ggcgccggac gtctacaccg acctcgccgc
     6781 cgaggccgcc agccgcgccg agctgcgcga gctgctgccc gccctgcggg cgcgggccgg
     6841 gaccacctcg acgccggcgc tgatccgcgc cttcggctcg ctgc
//
LOCUS       DASPJI010000295        22246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_312021, whole genome shotgun sequence.
ACCESSION   DASPJI010000295 DASPJI010000000
VERSION     DASPJI010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 22246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22246)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22246
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312021"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VEL74_07770"
     CDS             <1..978
                     /locus_tag="VEL74_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62464.1"
                     /translation="DSLATGTAAVNGSVEAGREGPDGRLQVRFDRFNLQSLRLRGRPA
                     YVRGRGLRLDVTSRSSVDLTAPVADIRASIDLPSGEVPDLRVFNPYLPADAGIAVLGG
                     RGRVKLHWDLDGATKAGQGRAELSSQAARLRFQGLDIDGSFALRMPMTTKDLESRRFE
                     LGGTELSLARISYYNTANGNGNGNGEGKDWWARLDLPQASATWEEPLRLRGTGTIAMK
                     NADPLLLLFAPRRQFLRWFQGALGVENVVAKGSFRLEGDALEIPALQASGGALDLQTR
                     MLFSKSRRVGDLLIEYGRLATGIELRDGQRTFKLRKPREWYEESGKGFR"
     gene            complement(1031..1645)
                     /locus_tag="VEL74_07775"
     CDS             complement(1031..1645)
                     /locus_tag="VEL74_07775"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HYG62465.1"
                     /translation="MKITNTASALLLAGALVTSACATQSDTQTGTAAGTDAGMAAGQQ
                     TTGDQGAALAAQDRTFAMQAASGGMLEVQLGQLASQKAANAQVRSFGQRMVTDHSQAN
                     QRLMQVAQNKGLDLPTQMMPEHRAHLDMMAPLSGAEFDRMYMSHMIQEHNKDISSFEQ
                     QTRSGQDPDLRSFAQQTLPTLRQHRELAQSIASSVGASQQGTGE"
     gene            complement(1772..2086)
                     /locus_tag="VEL74_07780"
     CDS             complement(1772..2086)
                     /locus_tag="VEL74_07780"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYG62466.1"
                     /translation="MSYLVLWSDFALDRLTEFLDFIAQDNPSAARRVVQDLFDRVQAL
                     ADQPWLGHRLSDAAHTSMRRLVVGAYVLVYQVDEPAQTIWILAARHSRQRPFPEEGRS
                     GR"
     gene            complement(2086..2370)
                     /locus_tag="VEL74_07785"
     CDS             complement(2086..2370)
                     /locus_tag="VEL74_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HYG62467.1"
                     /translation="MARIQLNLTEDIQPSSDLGAHGEALLRKVQDTRRPVVLTEHGKG
                     AAVLVDLESYQSLLEELELLRDVHRGLADVQAGRVVPHDEARARLMARYA"
     gene            complement(2400..3569)
                     /locus_tag="VEL74_07790"
     CDS             complement(2400..3569)
                     /locus_tag="VEL74_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYG62468.1"
                     /translation="MATAPPLTIPGTFAGSAFDPSLPDGRGAGRISIAGGALRFESGQ
                     HHLELPLAGLELRLGGENDAYLFASHPSRPGTTLSTKELAILDHPQITGDPELARQVA
                     RMRSKSRRTRVSCGAALLVTLAVVVGLIAGVVLLKDRLVDAVAKRVPPSLETRLGDAV
                     FSQIRASGNLVEDPEVQARVERLVAPLVAAVPEKQYPLRFHVLKDPTVNAFALPGGNM
                     VLHTGLVLRAERPEEVLGVLGHEIAHVTRRHTLRQMVQTAGTFVIVQAVIGDVEGLMA
                     VLTDSGTRLLQLRFSRDFEREADDTGWQYLIDGGIDPRGLITFFEKMRADEQEQLGEA
                     ANAMGAMDFLSTHPASDERIERLTAKLRQLESGSERRWKELDVDFTGLQQRLKGL"
     gene            3595..5454
                     /locus_tag="VEL74_07795"
     CDS             3595..5454
                     /locus_tag="VEL74_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYG62469.1"
                     /translation="MRDEIPGGAEGVLQALPLGVLVVGPDLAIRQANRAACALVGLAE
                     SALLGLGWRDVFPDASHGDGFPLPPRGLPLPASPIRGLVGHRAAGGGELTWLEVEAMP
                     VAGAAGAPGVDHVVCTLRDVTRNAGGAEGWEERLRVEEQLLQARSEWTATVDTVADMI
                     LLEDSRGRVLRCNQAAARFFGATYTGIVGRHLNELFFPGEIGEDTMAEVHPLFRTASA
                     VVQFPGPGHQDQWYEITNYPLAVRIARGGAGWVHVLENVTARREAEILAQRLAAAIDQ
                     AVESIVILDTEGRVEYINPAFSVQTGIGAEILGRRLRSFPVGPVDRQVYREIARTLLR
                     GQVWQGTYAARGGDGAVYQEEATISPVRDAAGTVLDYVAVCRDITERRRLESIAEAVN
                     MMDSVGYIFSNLRHELGNPVNSIKTALSVLRKNLDQYPRETVESYLDRTLAEVGRVEY
                     LLKALRSFNMHEKPRVERLDMGTFLADFHALVREDFAGKGIAVEADVPAESWEALADP
                     RALHQVLLNLLTNAADAVVGRETPRIVLRLERRPRSVDLAVVDNGAGIPDSQRAELFR
                     PFHTGKPHGTGLGLVIVKKLVTQMGGTVEVTSEVGVGTEVRVSLRAALEAVEG"
     gene            5512..6633
                     /locus_tag="VEL74_07800"
     CDS             5512..6633
                     /locus_tag="VEL74_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG62470.1"
                     /translation="MPTSKPPHRHTVLIADDEPLFLSSVAEGLRTLDADYEVLVASNG
                     REAVALVNAHPIDLVVTDIKMPELDGFGLIGWLLENHPGTPVIVMTAFGTPDIEEKVR
                     SLGAIGYLEKPIDFQTLTERIEQALQRSARGHIQGITLFSFLQLLQMERKTCTLEVAS
                     KGRKARLHFQEGELIDAEAGDLRGEEAVQALAGWEAPKIEIDNVCRAKKKTITASLTN
                     LLLEAARLEDEKAKGIRSRPAAAAKAPAGAKGKEPAGRRTSLDHFLKLDGVLAAVLVD
                     LESATSQASASRDLGFDAAAVASGVAGLVRADRAMAQDLDLGRGARELLLTLEDRLHL
                     IRLSGRAPRLALFLALDRDEASLGMLRLDLEDLEESLGL"
     gene            complement(6641..6979)
                     /locus_tag="VEL74_07805"
     CDS             complement(6641..6979)
                     /locus_tag="VEL74_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HYG62471.1"
                     /translation="MPRDLRVYLDDILEAARKIRDYTAGLSFEAFQQDEKTIDAVVRN
                     LEVIGEAVKSIPDGLRARHPDVPWKKISGLRDILIHEYFGIDLELIWDVVQAKLPILV
                     GRVSDLLESD"
     gene            complement(6963..7265)
                     /locus_tag="VEL74_07810"
     CDS             complement(6963..7265)
                     /locus_tag="VEL74_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYG62472.1"
                     /translation="MEKRNQILDTLERHGESLRSLGVQRIGLFGSHARGDARENSDLD
                     FLVDFRPGAKSFDNYMDLKAFLEDLFGARVDLVIPETLKARLRDGILRETVYAPGP"
     gene            7487..7924
                     /locus_tag="VEL74_07815"
     CDS             7487..7924
                     /locus_tag="VEL74_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010627717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HYG62473.1"
                     /translation="MDPFMRAAIDEARQGLREGGIPIGSVLVHEGRVLGGGHNRRVQQ
                     GSTVLHGEMDALERAGRQPASVYRECTLYTTLSPCAMCTGAILLYGIPHVVIGENRTF
                     LGEEELLRSRGVEVQVLQDAECIQMMTDFIRDNPTLWNEDIGR"
     gene            8136..8552
                     /locus_tag="VEL74_07820"
     CDS             8136..8552
                     /locus_tag="VEL74_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HYG62474.1"
                     /translation="MFNNAKRHIFIALLLIGLMSFGAVAAEARDRDTNGSDRLLKGAA
                     VGAAVGAVTQAVRGRTEGRELLKGAAVGGAVGAAVGAYSDYKQEKNAREDERHDRRGR
                     YYEGRSYRGSRRGDYYPRDNRARRGHRHTAHCSHRR"
     gene            8672..9946
                     /locus_tag="VEL74_07825"
     CDS             8672..9946
                     /locus_tag="VEL74_07825"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HYG62475.1"
                     /translation="MLKFLHAADVHLDSPLKGLERYEGAPVEEIRGATRRAFENLVEL
                     ALAEEVAFLLLAGDLYDGDWKDYNTGLFFASQMRRLEEAGIPVFVIAGNHDAASQITR
                     VLRPPANVRVLSTKRPETVVLAELEVALHGQGFVSAAVSDDLSAVYPAAEPHLFNVGL
                     LHTSLDGRPGHAAYAPCTVDGLRSRGYQYWALGHVHEREVVCRDPWIVFPGVLQGRHA
                     RETGAKGATLVTVQDREVTAVEAHDLDVVRWAVCPVDAAGAGSAGEVLDRVQRALERE
                     VAAAEGRTLAARLVIEGACPAHGELVSAPERWVQECRSLAFAFGEEAVWIEKVRIRTR
                     QPAAALDPFAADDALGGLLRSIRDLEADDAELAELAGELAELRRKLPAELFEPADTGE
                     ALDPADPAQLRGVLADVRDLLLARLLATPEGA"
     gene            9943..13455
                     /locus_tag="VEL74_07830"
     CDS             9943..13455
                     /locus_tag="VEL74_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYG62476.1"
                     /translation="MRLLELHLKAFGPFTGRRLDLSGGEHGLHLVFGPNEAGKSSALR
                     ALKALLFGFPARSPDDFLHSYDQLRVGGRLLLADGSEVSFLRRKGSKNTLLSDAEESP
                     LDGAFLDRCLQGMEEAHFSNLFGIDHGALVGGGRELLAQHGEVGQALFAAGLGSRSLR
                     RVLQSLDEESEGLFLPRGTKPLINQALAEYKETKRELSDASLSGRKFEDLRKERERLR
                     GEAEALAAEIAARGREKNRLERLRRALPLLAERRRLLARLGELEGAAVLPPEFPERRQ
                     RVQEALRKAREDGRRAAAEREGLAEEAAGLTVAAPVLAQAETIERLHRDVGRFGKDST
                     DRVRLQGEVAELRAQAAELLDEVRPGLSLEEAESLLPASYRWMRIQELSNQRQALVNG
                     LDQARRAAAEAASALEAARDALARLPDRRDPGLLRRRAEAARRAGSLDDQRAEAAAAL
                     RSEGEQLALGLGRLGLWSGTAEELESLPVPPAETLERFGRDLDVLAERRRTLEERRRA
                     AREALGEAIRQLEMLHRSGSVPAEDDLARARQRRDQGWDLLRRQWLEGEDVAAEARRF
                     DPARPLPDAYEQTVEAADDLADRLRREADRVQLQARYTAERDRWDSEAASLDAEVAAL
                     AEEAGRAESAWKELWRPCGLDPLPPREMHPAWTARYEKLRARAEAIRAGRRRLEGIEE
                     AIARHREALMEALAELGETAPGSERLEPTLALAEDIVRRLEGEERQRVRQEEAVREAE
                     GRLAGARRDEEAALAARERWSGEWAETIRDLGLGGDALPSEVSQLVASLQKISEARRN
                     AEKIERRIGGLARDIDTFRDAVRALAAEFAAERLTHPPDQTAVHLHALLQDARRRADR
                     REALEQRMEKLGRELLEAEATRRAMEERLAELRQEAGCAEEADLADLDEAERRSAEVR
                     DLRRDLDRNERQLLEAGEGVALDDLGREAEGVDTDELPGRIEQLEREVEEREGRLGDF
                     REELGRRGRELEELKGGDAAAQAAERAEEVMARLRDGVDRYVRLRLAGILLRRRIERY
                     RAENQAPLLRRAGELFATLTLGSFAGLQTDYDEKDDPVLVGVRAEGQRVRVEGMSDGA
                     RDQLYLALRLATLEKHLAHAEPLPFVVDDILINFDDRRSEATLRVLAELSKRTQVILF
                     THHERLKEMAGALRNGAGVFVREL"
     gene            13555..14112
                     /locus_tag="VEL74_07835"
     CDS             13555..14112
                     /locus_tag="VEL74_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016558099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HYG62477.1"
                     /translation="MNNLQGKKVAILVTDGFEQVELTEPRKALDEAGARTEIVSPKKD
                     KVKGWNHTEWGQELPVDTPLAEAKPEQFDALLLPGGVMNPDHLRRDPAVQRFVKSFFD
                     EGKPVAAICHGPWTLIDAGVVRGRKMTSYETIQMDLKNAGVNWVDEEVVVDGNLVTSR
                     KPDDIPAFNRAMVEQFAGQEVGAGR"
     gene            15130..15960
                     /locus_tag="VEL74_07840"
     CDS             15130..15960
                     /locus_tag="VEL74_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYG62478.1"
                     /translation="MDYLSFQPDFESPDAVAAYDELPLWSAMFGLLLLRHVPLRRGIS
                     VLDVGCGTGFPLLELAQRLGPTCRVHGLDPWEPALERARFKARVWNVRNVEIHQGTAE
                     AMPFPDATFELLVSNLGVNNFDRPEAALAECRRVAKPDARLVLTTNLRGHMEELYQVY
                     AETLRQLGREADLPTLERHVDHRASVESVTTLLETHGFRVVHVHRETETMRFLDGSAL
                     LRHYFIQLGFLGGWKGVVAPSDQAEVFKALEENLNRRAEARGGLGLTIPMVCVEAERG
                     "
     gene            15997..16287
                     /locus_tag="VEL74_07845"
     CDS             15997..16287
                     /locus_tag="VEL74_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HYG62479.1"
                     /translation="MTSKGQITIPKEIRVALGLRAGSRLFLRMREDGVVEISSETVDL
                     MSLCGILKPAVRGVTLENLEAAIRDGAAGEESESGNSMKQVSKGAPPPRSSP"
     gene            complement(16332..17051)
                     /gene="mtnC"
                     /locus_tag="VEL74_07850"
     CDS             complement(16332..17051)
                     /gene="mtnC"
                     /locus_tag="VEL74_07850"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017711783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HYG62480.1"
                     /translation="MALPEGVRAVLTDIEGTTTPISFVYDVLFPYAEARLDAYCGRPD
                     QTPELAEALERLRQEHREDRERSAETPDFGDGAPYARHLMAQDRKSTGLKLLQGIIWD
                     EGYRTGELRSVVFPDVPVALEAWKDAGLRLRVFSSGSVHAQKLLFAHTAYGDLTRLFE
                     GYHDTTTGPKKEARAYTAIAEAYGLPAADILFLSDVVDELDAAAEAGMRTGLLIRPGN
                     RPTEAGGHPVYTTFDELLPTA"
     gene            complement(17054..17620)
                     /locus_tag="VEL74_07855"
     CDS             complement(17054..17620)
                     /locus_tag="VEL74_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYG62481.1"
                     /translation="MATLTFRESGRVVEDPAEIAGILSGVGIWYRRFEGSDQLPEDAA
                     DQEILDAYREPIQRLMAEGGYVTADVINVKPSTPGLETMLNKFSKEHWHDEDEVRFIV
                     HGRGVFHIHPPEGEVFGLEVVKGDMIRVPRGTHHWFDLCADKTIRAIRLFQDPSGWTP
                     HYTETGVDTNFQPLCFGPNYFPPPARTL"
     gene            17819..18361
                     /locus_tag="VEL74_07860"
     CDS             17819..18361
                     /locus_tag="VEL74_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62482.1"
                     /translation="MEHPKRPGLFWRRGDGLHVLALGSLVMVGATCCHSMAGVPTQHI
                     VMFDSHGKAMDPTGKIDCEEPVPPKVEARTADQLMTPGEPDWNRCNGKISRLSSFKQL
                     DSREFSKYLDQVFRSIPATPGAPSPVSSTTGVTGRRASDFRRRRSARALGSFARMIRK
                     PGNRPGGGDRAALARVPAPC"
     gene            18375..19013
                     /gene="mtnB"
                     /locus_tag="VEL74_07865"
     CDS             18375..19013
                     /gene="mtnB"
                     /locus_tag="VEL74_07865"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014275409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HYG62483.1"
                     /translation="MAPPSAGSDPREVLIAASRLLYGRGWMAGTAGNLSVREGEGPFW
                     ITASGKAKGTLGRADFVRVSLDGEVVERGGPDSRPSAETSIHGAAYRLFPEARACYHV
                     HTIPTNLVTRFTEGDELPLPPIEMIKGLGVWEQEPRVSVAVLPNHLDVPRIAAEMEAR
                     FRAAAPRLPGFLIRDHGLTVWAQSPEAALNYLELFDYIFGCMVAARSSDLRW"
     gene            19176..>22246
                     /locus_tag="VEL74_07870"
     CDS             19176..>22246
                     /locus_tag="VEL74_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62484.1"
                     /translation="MVDMTSQAGELSPKQRRLLELMLKQKQKEKEAGAQTPAALPRRQ
                     PSDPLVLSFGQKRLWFIEQLQPGSPAYHVPGAVRIRGPLNVPALEAALAEVARRHEIL
                     RTRFHVVEGQPALDIEPEPALAVEVVDLRPVPAERREEELAYQVDARVLQPFDLSRTP
                     LARTVLYRLGDEEHVFLLILQHIICDIWSVGVFFRNTMALYDAFLAGSPSPLPELPLQ
                     YTDYALWQERLLQGEGLERLLSYWKGELAGAPFVIDLPTDHPRPALQSFEGSRRYMNM
                     SVELTEALKAVAKAQDASLYMILLAALYALLHRYTGQESILVGVPMANRGRVELEGLV
                     GLLFNSLVLRADLGPEMPFRDLLSQVRERTLGAFNHQDLPFEKLVEELRVERDMSRNP
                     IYQVLFAFQNVPPSTMAARDLAIQRYEVRETASREDLELDMRETPEGLAGWFGYDRAL
                     FDASTVTRTTLHFQNLLAGIAADPARPLAELPLLTASETQALLFEGNDTGVTDTAEES
                     FEALFEARVAETPSAPAAVFGGTPETYAELSARANRLARHLVGMGIGPGVVVGLLDRR
                     GPDLLAAILGVEKAGGAWLPLDPEHPARRHAQVLAASATPLVLAHEDLLPVLTEAVAE
                     LPEGRHPRLEVLADLLARPGDESTPPRRAAPDDLAYTLFTSGSTGVPKGAMVTRRGML
                     NHLRAKIEDLGLGPSDAVAQTASQCFDISVWQFLAALAVGGRVHIYEDETAHDPARLL
                     ARVETDGVTVVETVPSLLRLMLEEAERSGGQSRPGALRWMIPTGEALPPELVDRWLRR
                     FHAIPLVNAYGPTECSDDVTHQRLDGPLGDGTARVPIGTPIRNTRLYVVDRGLRLVPM
                     GVAGELCVGGEGVGRGYLGAPDKTAAVFVPDPFAGPSGQDGARLYRTGDLARRLAGGA
                     FDFLGRIDHQVKVRGFRIELGEIESVLAQHPAVRQAVVLAREAGASERALVAYVVAQA
                     GEETERELRDFLQTWLPDYMVPAAFVTLEALPLTPNGKVDRRALLALGGPEL"
BASE COUNT         3323 a         7702 c         7917 g         3304 t
ORIGIN      
        1 gacagcctgg cgaccggcac ggcggcggtg aacgggtcgg tcgaggcggg ccgggagggg
       61 ccggacgggc ggctccaggt gcgcttcgac cgcttcaacc tccagtcttt gcggctgcgc
      121 ggccggccgg cctacgtccg cggccgcggc ctccgtctcg atgtgacctc ccggtcgtcc
      181 gtggacctga ccgcgccggt ggcggacatc cgggcctcca tcgacctccc ctccggcgag
      241 gtgccggacc tgcgggtgtt caacccctac ctgccggcgg acgcggggat cgcggtcctc
      301 ggcggccggg gccgggtgaa gctccactgg gatctcgacg gcgccaccaa ggcggggcag
      361 gggagggccg agctgtcctc ccaggcggcg cggctccgct tccaggggct ggacatcgac
      421 ggcagcttcg ccctgcgcat gccgatgacc acgaaggacc tcgagagccg ccggttcgag
      481 ctgggcggca ccgagctctc cctggcgcgg atctcctact acaacacggc gaacggcaac
      541 ggcaatggca atggggaagg gaaggactgg tgggcgcggc tggacctccc ccaggcgtcg
      601 gcgacctggg aggagccgct gcgactgcgc ggcaccggca ccatcgcgat gaagaacgcc
      661 gaccccctgc tgctgctctt cgcgccgcgc cggcagttcc tgcgctggtt ccagggggcg
      721 ctcggggtcg agaacgtggt ggcgaagggg agcttcaggc tggaggggga cgccctggag
      781 atcccggcgc tccaggccag cggcggcgcg ctggatctgc agacgcggat gctgttctcg
      841 aagagccggc gggtgggcga cctgctgatc gagtacggcc gcctggcgac cggcatcgag
      901 ctgcgcgacg gccagaggac gttcaagctg cgtaagccgc gggagtggta cgaggagagc
      961 gggaagggat tccggtgagg ggagggaagg gaagggaggg ccggcgaagc cgccggcccc
     1021 cggggtcgtg ctactcgccc gtaccctgct gggaggcgcc gacggacgac gcgatgctct
     1081 gggccagctc acggtgctgg cgcagcgtcg gcagcgtctg ctgggcgaag gaacggaggt
     1141 ccggatcctg gccgctccgg gtctgctgct cgaagctgct gatgtccttg ttgtgctcct
     1201 ggatcatgtg gctcatatac atccggtcga actcggcgcc cgagagcggg gccatcatgt
     1261 cgaggtgagc ccgatgctcg ggcatcatct gcgtgggcag gtcgagccct ttgttctggg
     1321 ccacctgcat cagccgctgg ttggcctggg agtggtcggt caccatgcgc tggccgaacg
     1381 agcggacctg ggcgttggcc gccttctggg acgcgagctg gccgagctgg acctccagca
     1441 tgccgccgct ggcggcctgc atggcgaagg tgcggtcctg ggcggcgagg gcggcaccct
     1501 ggtcgcccgt ggtctgctgg cccgccgcca tgccggcatc ggtcccggcg gcggtcccgg
     1561 tctgggtatc cgactgcgtg gcacaagcgg aggtgacgag ggctccggcg aggagcaatg
     1621 cactggctgt gttcgtgatc ttcatggcgt ctccttttac aatgatgaaa ttcctggaag
     1681 tcaggagaga aggaaaggcg gccccccaag gccgccatca cttcaagaag ttttcaactg
     1741 caaagggagt gccaggaggg ctgacggagc gtcagcggcc agagcggcct tcttctggga
     1801 aggggcgttg gcgtgagtgc cgagctgcca agatccagat cgtctgtgcc ggctcatcga
     1861 cctggtagac gagaacgtac gctccgacca caaggcgccg catgctggtg tgggctgcat
     1921 cggaaagacg gtgccccagc cagggttgat ctgccagagc ttggacccgg tcgaagagat
     1981 cctggacgac ccgtctcgcc gccgagggat tgtcctgagc gatgaagtcg aggaattcgg
     2041 tcagccggtc cagagcgaaa tcggaccaca ggacaagata ggacactatg cgtatcgggc
     2101 catcaaccgt gccctggcct catcatgagg tacgacacgt ccggcttgga cgtctgccaa
     2161 cccgcggtgc acgtcccgaa gcagctcgag ctcttcgagc aggctttgat agctctccag
     2221 atctacgagg acggcggcac ccttgccatg ttccgtcagc acgaccgggc gcctggtgtc
     2281 ttggactttg cggaggagag cctcgccatg ggccccgagg tcggaagagg gttggatgtc
     2341 ttctgtcaga ttgagctgga tacgggccat ggtaaacctt tccaagtttc cctagcttac
     2401 tacagaccct tcagccgctg ctgcagcccc gtgaagtcga cgtccagctc cttccagcgg
     2461 cgttcggagc cggattcaag ctggcgcagc ttggcggtga gccgctcgat gcgttcgtcg
     2521 gaggcggggt gggtgctgag gaagtccatc gctcccatgg cattggccgc ctcgccgagt
     2581 tgctcctgct cgtcggcccg catcttctcg aagaaggtga tgagcccgcg cgggtcgatc
     2641 cccccgtcga tcaggtactg ccagccggtg tcgtccgcct cgcgctcgaa gtcgcgggag
     2701 aagcggagct ggaggagccg cgtgccgctg tcggtgagga cggccatcag cccctcgacg
     2761 tcgccgatca cggcctggac gatcacgaac gtgcccgccg tctggaccat ctgccgcagc
     2821 gtgtggcggc gcgtcacgtg ggcgatctcg tggccgagca cgcccagcac ctcctccggc
     2881 cgctcggcgc gcagcacgag gccggtgtgc aggaccatgt tccctcccgg cagggcgaag
     2941 gcgttgaccg tggggtcttt caggacatgg aagcggaggg ggtactgctt ctcgggcacc
     3001 gcggccacca ggggggccac cagccgctcg acgcgcgcct ggacctccgg atcctccacg
     3061 aggttgccgc tcgcccggat ctgcgagaag accgcgtcgc ccagccgggt ctcgagcgac
     3121 ggcggcaccc gcttcgccac cgcgtccacc agccggtcct tgagcaggac gacgccggcg
     3181 atcaggccga cgacgaccgc cagggtcacc aggagcgcgg cgccgcagga gacgcgggtc
     3241 cgccggctct tcgagcgcat gcgcgccacc tgccgcgcca gctccgggtc gccggtgatc
     3301 tgcgggtgat cgaggatcgc cagctccttc gtggagaggg tggtccccgg ccgcgacgga
     3361 tgggaggcga agaggtaggc gtcgttctct ccccccaggc gcagctcgag gccggccagg
     3421 ggcagctcca ggtggtgctg gcccgactcg aagcgcaggg cgccgcccgc gatcgagatc
     3481 cgtccggcgc cgcggccgtc ggggagagat ggatcgaagg cggagccggc gaaggttccg
     3541 gggatcgtaa gcgggggagc ggtggccata tccactaaga tatccccaac cgatatgcgc
     3601 gacgagatcc ccggcggcgc ggagggcgtg ctccaggccc tccccctggg cgtgctggtg
     3661 gtggggcccg accttgccat ccggcaggcc aaccgggcgg cgtgtgccct ggtgggcctc
     3721 gcggaatccg cgctgctggg cttgggctgg cgcgatgtct tccccgacgc ctcccacggc
     3781 gacggcttcc cgctgccgcc ccgcggcctg ccgctgccgg cctcgccgat ccgcgggctc
     3841 gtgggacacc gcgcggccgg cggcggcgag ctgacctggc tggaggtcga ggccatgccg
     3901 gtggccggcg ccgccggagc ccctggggtg gatcacgtgg tctgcaccct ccgcgacgtc
     3961 acccgcaacg ccggtggcgc cgaaggctgg gaggagcggc tgcgggtcga ggagcagctc
     4021 ctccaggcgc gcagcgaatg gaccgccacg gtggacacgg tggcggacat gatcctgctc
     4081 gaggactcgc gcggccgggt actgcgctgc aaccaggcag cggcccggtt cttcggcgcc
     4141 acctacaccg gcatcgtcgg ccggcacctg aacgagctgt tcttccccgg cgagatcggg
     4201 gaggacacca tggccgaggt ccaccccttg ttccgcaccg cctcggcggt ggtccagttc
     4261 cccggccccg gccaccagga ccagtggtac gagatcacca actatccgct ggcggtgcgc
     4321 atcgcccgcg gcggcgccgg ctgggtgcac gtgctggaga acgtcaccgc ccggcgagag
     4381 gccgagatcc tggcccagcg cctcgccgcc gccatcgacc aggcggtgga gtcgatcgtc
     4441 atcctcgaca ccgaggggcg ggtggagtac atcaaccccg ccttctccgt ccagacgggg
     4501 atcggcgccg agatcctggg gcgccgcctg cgctccttcc ccgtcgggcc ggtcgaccgc
     4561 caggtctacc gggagatcgc ccggacgctc ctgcgcggcc aggtctggca ggggacctat
     4621 gctgcccgcg ggggggatgg cgccgtctat caggaagagg cgaccatctc gccggtgcgc
     4681 gacgccgccg gcaccgtcct cgactacgtg gccgtctgcc gcgacatcac cgagcgccgg
     4741 cgcctggagt cgatcgccga ggcggtcaac atgatggaca gcgtcggcta catcttctct
     4801 aacctgcgcc acgagctggg caatccggtc aactccatca agaccgccct ctcggttctg
     4861 cgcaagaacc tggaccagta cccgcgggag acggtggaga gctacctgga ccgcaccctc
     4921 gccgaggtgg gccgggtcga gtacctcctc aaggcgctgc gcagcttcaa catgcacgag
     4981 aagccgcggg tggagcggct ggacatgggg accttcctgg ccgacttcca cgctctggtg
     5041 cgggaggact tcgccggcaa ggggatcgcg gtcgaggccg acgtcccggc cgagtcctgg
     5101 gaggcgctgg ccgatccccg cgccctccac caggtgctgc tcaatctcct gaccaacgcc
     5161 gccgacgcgg tggtagggcg agagacgccg cgcatcgtcc tgcggctgga gcgccggccg
     5221 cgcagcgtcg acctggcggt ggtggacaac ggcgccggca tcccggattc acagagggcg
     5281 gagctgttcc gtccgttcca caccggcaag ccccacggca ccggcctggg attggtcatc
     5341 gtcaagaagc tggtgaccca gatggggggg acggtggagg tcacgagcga ggtgggggtc
     5401 gggaccgagg tgcgcgtctc cttgagagcg gccctggagg cggtggaggg atgagcctgc
     5461 ttgccaccgc cccgttccgg ctggcagact gcactcttct ctgagacgcc catgccgacc
     5521 tccaagcccc cccaccgcca caccgtgctc atcgccgacg acgagccgct cttcctgtcg
     5581 agcgtggccg agggcctgcg cacgctcgac gccgactacg aggtgctggt cgcgtcgaac
     5641 ggccgtgagg cggtggcgct ggtgaacgcc catccgatcg acctggtggt caccgacatc
     5701 aagatgccgg agctggacgg cttcgggctc atcggctggc tgctggagaa ccatccgggg
     5761 acgccggtca tcgtgatgac cgccttcggc acgccggaca tcgaggagaa ggtccgctcc
     5821 ctgggcgcca tcggctacct cgagaagccg atcgacttcc agaccctgac cgagcgcatc
     5881 gagcaggcgc tgcagcgctc cgcccgcgga cacatccagg ggatcaccct cttcagcttc
     5941 ctgcagctcc tccagatgga gcgcaagacc tgcaccttgg aggtcgcctc caaggggagg
     6001 aaggcgcggc tccacttcca ggagggggag ctgatcgacg ccgaggcggg cgacctgcgg
     6061 ggggaggagg cggtgcaggc cctggccggc tgggaggcgc cgaagatcga gatcgacaac
     6121 gtctgccgcg ccaagaagaa gacgatcacc gcctcgctga ccaacctcct cctggaggcg
     6181 gcccggctgg aggacgagaa ggcgaagggt atccgctccc gtcccgccgc ggccgccaag
     6241 gcgccggcgg gagcgaaggg caaggagccc gccggccgcc ggacctcgct ggaccacttc
     6301 ctcaagctgg acggcgtgct ggccgcggtg ctggtggacc tcgaatccgc caccagccag
     6361 gcctcggcgt cgcgggacct cggcttcgac gcggcggcgg tggcctcggg agtcgccggc
     6421 ctggtgcggg cggaccgcgc catggcgcag gacctggacc tcggccgcgg cgcccgcgag
     6481 ctgctcctca ccctggagga ccgcctccac ctcatccgcc tctccggtcg cgcccctcgc
     6541 ctcgccctct tcctcgccct cgaccgcgac gaggccagcc tcggcatgct gcggctggac
     6601 ctggaagacc tggaagagtc gctggggctg taggggagga ctagtcggac tcgaggaggt
     6661 ccgagacccg ccccacgagg atgggcagct tggcctgaac gacatcccaa atcaattcga
     6721 ggtcgatccc aaagtactca tggataagga tgtcccggag gcctgaaatc ttcttccaag
     6781 ggacatcggg atgtcgagcc ctaagaccgt ccggaatgct cttgaccgct tctccgatca
     6841 cttcgagatt ccggaccacg gcgtcgatgg tcttctcgtc ctgctggaag gcctcgaaag
     6901 aaaggccagc cgtgtagtcc cggatcttgc gagccgcttc gaggatgtcg tccaggtaga
     6961 ctctaaggtc ccggggcata aaccgtctcg cggaggatgc cgtcccggag gcgtgccttc
     7021 agtgtctcgg ggatgaccaa gtccacccgg gcgccgaaga gatcttcgag gaaggccttc
     7081 aggtccatgt agttgtcgaa gctcttcgct ccaggcctga agtcgaccag gaagtcgaga
     7141 tcgctgtttt cccgagcatc accgcgagcg tgcgaaccga agaggccgat ccgctggacg
     7201 cccagcgacc ggagcgattc cccgtgcctc tccagggtgt cgaggatctg attgcgcttc
     7261 tccatctacc cgattgtacc cctggcaccc atctggcagc acccacggtg ccgcccgccg
     7321 gtccgggcgt cgacccaggg gagggatgta tgccggtcaa ggtccgcatg gtaggctgct
     7381 ggacgagtat catcctgtcg atcgtcctca ccgtgctgct caacctgatg ctccgcggct
     7441 gctccggcgg cggctggtga cggacgcctt ctctcggagg tgagcgatgg acccgttcat
     7501 gcgcgccgcc atcgacgagg cccgccaggg tctgcgcgaa ggtggcatcc ccatcggctc
     7561 ggtcctggtg catgaaggca gggtgctcgg cggcggccac aaccggcggg tgcagcaggg
     7621 gagcaccgtc ctgcacggcg agatggacgc gctcgagcgg gccggccgcc agcccgcctc
     7681 ggtctaccgc gagtgcaccc tctacaccac cctctccccc tgcgccatgt gcaccggcgc
     7741 catcctcctc tatggcatcc cgcacgtggt catcggcgag aaccggacct tcctcggcga
     7801 ggaggagctc ctgcgcagcc gcggcgtcga ggtgcaggtg ctccaggacg ccgagtgcat
     7861 ccagatgatg acggacttca tccgcgacaa cccgaccctc tggaacgagg acatcgggcg
     7921 ctgacgcctg gagggggcgg gccgtcctcc tcagcggacg ttccagagcc gcggagcagg
     7981 ctcccgaaga cgtctcgaga ccctcaactt cttctgccag aagagcttgc aggtgttcag
     8041 cctcacatgg catagctctt gatctatatg gctggtgacg gcgccggagt tgtcccggca
     8101 acgtcagctc cactggtaag gagaaggaga acgagatgtt caacaacgcg aaacgccata
     8161 tcttcattgc actgctcctg atcgggctca tgtccttcgg cgcggtggcg gccgaagccc
     8221 gagaccgcga cacgaacggc tcggaccggc tcctgaaggg cgccgccgtc ggcgctgccg
     8281 tgggcgccgt cacccaggcc gtccgcggac ggaccgaagg ccgtgagctc ctgaaggggg
     8341 cggcggtcgg tggcgcggtg ggtgccgccg tcggcgccta cagcgactac aagcaggaga
     8401 agaacgcccg cgaggacgag cgccatgacc gccggggtcg ctattacgag ggccgctcct
     8461 accgcggctc ccgccggggc gactactacc cgcgcgacaa ccgcgcccgc cggggccacc
     8521 gtcacacggc ccactgctcg caccgccgct aagcgcgagt agaagccggg caaggctggg
     8581 cgcctgatgc cgcaaggccc accggagcgt cgcctccggt gggcctttcc gttcctgctg
     8641 tatgcttctc cgagccctgc agcaggcccg gatgctcaag tttctccacg ccgcggacgt
     8701 ccatctcgac agccccctga agggcctgga gcgctatgaa ggcgcgccgg tggaggagat
     8761 ccgcggcgcc acccgccggg cgttcgagaa cctggtggag ctcgccctcg ccgaggaggt
     8821 ggccttcctc ctgctcgccg gcgacctcta cgacggcgac tggaaggact acaacaccgg
     8881 cctcttcttc gcttcccaga tgcgccggct ggaggaggcg ggcatccccg tcttcgtcat
     8941 cgccggcaac cacgacgcgg cgagccagat cacccgcgtg ctccggccgc cggccaacgt
     9001 ccgggtgctc tccacgaagc ggccggagac ggtggtgctg gcggagctgg aggtggcgct
     9061 tcatggccag gggttcgtct cggcggcggt ctccgacgac ctctcggccg tctacccggc
     9121 ggctgagccg cacctgttca acgtcggcct gctccacacc agcctcgacg gccggcccgg
     9181 ccacgccgcc tatgccccct gcaccgtcga cggcctccgg tcccgcggtt atcaatactg
     9241 ggccctggga cacgtccacg agcgcgaggt ggtctgccgc gatccctgga tcgtcttccc
     9301 cggcgtcctc caggggcgtc acgcccgcga gaccggcgcc aagggggcga ccctggtcac
     9361 cgtccaggac cgggaggtga ccgcggtgga ggcgcacgac ctcgacgtgg tccgctgggc
     9421 ggtctgcccg gtggacgcgg cgggcgccgg cagcgccggc gaggttctcg accgggtgca
     9481 gcgggcgctg gagagggagg tggcggcggc cgaggggcgg acgctcgcgg cgcggctggt
     9541 gatcgagggt gcctgtccgg cccacggcga gctggtgtcg gcgcccgagc gctgggtcca
     9601 ggagtgccgg tcgctggcct tcgccttcgg cgaggaggcg gtgtggatcg agaaggtccg
     9661 gatccgcacc cgccagcctg ccgcggcgct ggaccccttc gccgcggacg atgccctcgg
     9721 cggcctcctg cggtcgatcc gcgacctcga ggccgacgac gccgagctgg cagagctggc
     9781 cggcgagctc gcggagctgc gccggaagct ccccgccgag ctgttcgagc cggcggacac
     9841 cggcgaggcg ctcgatcccg ccgatcccgc gcagttgcgg ggggtgctcg ccgacgtccg
     9901 ggacctcctg ctcgcccgtc tcctcgccac gccggagggc gcgtgaggct gctggagctc
     9961 cacctcaagg ccttcgggcc attcaccggc cggcggctcg acctgtcggg cggcgagcac
    10021 ggcctgcacc tggtcttcgg ccccaacgag gcaggcaaga gctccgccct gcgcgccctc
    10081 aaggccctcc tcttcggttt cccggcgcgc agcccggacg atttccttca ctcctacgac
    10141 cagctccggg tcggggggcg gctcctcctg gcggacggct ccgaggtctc cttcctgcgc
    10201 cgcaagggga gcaagaacac gctcctctcg gacgcggagg agtcccccct ggacggcgcc
    10261 ttcctcgacc gctgcctcca gggcatggag gaggcgcact tctcgaacct gttcggcatc
    10321 gaccacggcg ccctcgtcgg cggcgggcgc gagctgctag cgcagcacgg cgaggtgggg
    10381 caggctctct tcgccgccgg cctcggctcc cgtagcctgc gccgggtcct ccagtcgctg
    10441 gacgaggagt ccgaggggct cttcctgcct cgtggcacca agccgctgat caaccaagcc
    10501 ttggccgagt acaaggagac gaagcgggag ctgtccgacg cctccctctc cggccgcaag
    10561 ttcgaggatc tgcgcaagga gcgggagcgg ctgcgcggcg aggccgaggc gctggccgcc
    10621 gagatcgccg cgcgcgggag ggaaaagaac cgcctggagc gcctgcgtcg agccctgccg
    10681 ctcctcgccg agcggcggcg cctcctggcg cggctggggg agctggaggg ggcggccgtg
    10741 ctgccgccgg aattcccaga gcgccggcag cgggtgcagg aggcgctccg caaggcgcgg
    10801 gaggacggcc ggcgcgccgc cgccgagcgg gagggtctcg ccgaggaggc cgcgggcctc
    10861 accgtggcgg cgccggtcct cgcccaggcc gagaccatcg agcggctcca ccgggacgtc
    10921 gggcggttcg gcaaggactc cacggaccgc gtccgcctcc agggggaggt cgccgagctg
    10981 cgggcccagg ccgcggagct gctggacgag gtccgccccg gcctgagcct ggaggaggcg
    11041 gagtccctgc tccccgcctc gtaccgctgg atgcgcatcc aggagctgag caaccagcgg
    11101 caggcgctgg tcaacggcct ggaccaggcg cgccgggcgg cggccgaagc cgcgagcgcc
    11161 ctggaagcgg ctcgggacgc gctcgctcgc ctgccggatc ggcgggaccc cggcctgctg
    11221 cgccgccgcg ccgaagcggc ccgccgcgcc ggtagcctgg acgaccagcg ggccgaggcc
    11281 gccgcggcgc tgcggtccga aggcgagcaa ctcgcgctgg gtctcggccg tctcgggttg
    11341 tggagcggca ccgccgagga gctggagtcc ctccccgtgc cgccggccga gacgctcgaa
    11401 cgcttcggcc gcgatctcga cgttctcgcc gagcgccgga ggacgctaga ggagaggcgc
    11461 cgggcggcgc gggaggcgct gggcgaggcg atccgccaac tggagatgct gcaccggtcc
    11521 ggcagcgtgc ccgccgagga cgacctggcg cgggcgcggc agcggcgtga tcagggctgg
    11581 gacctcctgc gccggcagtg gctggagggg gaggacgtgg ccgccgaagc ccggagattc
    11641 gatccggccc gtccgctgcc cgacgcctac gagcagaccg tcgaggcggc cgacgacctg
    11701 gcggaccgcc tccgccgtga ggccgaccgc gtccagctcc aggcccggta cacggccgag
    11761 cgggaccggt gggactccga agccgccagc ctggacgccg aggtcgccgc gctggcggag
    11821 gaggccggcc gcgccgagtc cgcctggaag gagctctggc gcccctgcgg ccttgatccg
    11881 ctccccccgc gcgagatgca ccccgcctgg accgcgcgct acgagaagct ccgggcgcgg
    11941 gcggaggcga tccgcgccgg ccgccggcgg ctggagggga tcgaggaggc gatcgcgcgg
    12001 caccgggagg cgttgatgga ggcgctggcg gagctggggg agaccgcgcc cgggtccgaa
    12061 cggttggagc ccaccctcgc cctcgccgag gacatcgtcc gccggctgga gggggaggag
    12121 cggcagcgtg tccgtcagga agaggcggtg cgcgaggccg agggccgtct cgccggggcg
    12181 cgccgcgacg aggaggccgc tctcgccgcc cgggagcgct ggagcgggga gtgggcggag
    12241 accatccgcg acctcgggct cggcggcgac gccctgccgt ccgaggtcag ccaactcgtc
    12301 gccagtcttc agaagatctc ggaggcgcgc cgcaacgcgg aaaagatcga gcggcggatc
    12361 ggcgggctgg cccgcgacat cgacaccttc cgtgacgctg tccgcgccct ggccgccgaa
    12421 ttcgccgccg agcgtctgac ccacccgccc gaccagaccg ccgtccatct ccacgccctg
    12481 ctccaggacg cccgccgccg ggccgaccgc cgggaggcgc tggagcagcg gatggagaag
    12541 ctcggccgcg agctgctgga ggccgaagcc acccgccgcg ccatggagga gcggctggcc
    12601 gagctgcgcc aagaggccgg ctgcgccgag gaggccgatc tcgctgatct cgacgaggcc
    12661 gagcgccggt ccgccgaggt ccgggacctc cggcgcgacc tcgaccgcaa cgagcggcag
    12721 ctcctggagg cgggcgaggg ggtggcgctg gacgatctgg ggcgcgaggc cgagggggtg
    12781 gacaccgacg agctgccggg ccgcatcgag cagctcgaac gggaggtgga ggagcgcgag
    12841 ggccggctgg gcgatttccg ggaggagctg ggccggcggg gccgcgagct ggaggagctc
    12901 aaagggggcg atgccgccgc ccaggccgcc gagcgcgccg aggaggtcat ggcccggctg
    12961 cgcgacggcg tcgaccgcta cgtccggctg cggctcgccg gcatcctcct gcgccggcgc
    13021 atcgagcgct accgggcgga gaaccaggcc ccgctcctgc gccgcgccgg cgagctgttc
    13081 gccaccctca ccctcgggag cttcgccggt ctccagaccg actacgacga gaaggacgat
    13141 ccggtgctgg tcggcgtccg tgccgagggc cagcgggtgc gggtggaggg gatgagcgac
    13201 ggcgcgcggg atcagctcta tctcgccctc cgcctcgcca ccctggaaaa gcacctcgcc
    13261 cacgccgagc cgctcccctt cgtcgtcgac gacatcctca tcaacttcga cgaccgccgg
    13321 agcgaggcca ccctgcgggt cctggccgag ctgtcgaagc ggacgcaagt gatcctcttc
    13381 acccaccacg agaggctgaa ggagatggcg ggcgccctgc ggaacggagc gggggtgttc
    13441 gtgcgcgagc tgtaggtcgt cattccccgc ctggcaccct ggttgcttcg atgaactttg
    13501 agccctggag atctctctcc gggcgaagtc tcaccagcag ccaggaggtg catcatgaat
    13561 aacttgcaag gcaagaaagt cgcaatcctt gtcaccgacg gtttcgagca ggtcgagctg
    13621 accgagccgc ggaaggcgct cgacgaagcc ggggcgcgga ccgagatcgt ctcccccaag
    13681 aaggacaagg tgaaggggtg gaaccatacc gagtgggggc aggagctgcc ggtcgatacg
    13741 ccgctcgccg aagcgaagcc cgagcagttc gacgccctcc tcctgcccgg aggcgtgatg
    13801 aatcccgacc acctgcggcg ggatcccgcg gtgcaacgct tcgtcaagtc cttcttcgac
    13861 gaaggcaagc cggtggccgc catctgccac ggcccgtgga cgctcatcga cgccggggtc
    13921 gtccgcggcc ggaagatgac ctcctacgag acgatccaga tggacctcaa gaacgccggc
    13981 gtcaactggg tcgacgagga ggtggtcgtg gacggcaacc tcgtcaccag ccgcaagccg
    14041 gacgacatcc ccgccttcaa ccgcgccatg gtcgagcagt tcgccggcca ggaggtggga
    14101 gccgggcgct gacggcctcg ccgcagccgt tgcccgcgaa aaacgatcgt catccccggg
    14161 gagacgttca tcatctcccc ctggatggga cttcatcccc gcgggtaggg ccttcctacc
    14221 cagggtaagg tcttcatccc tccgggtacg ggctccatac ccgaggtacg gccttcatct
    14281 ccccgggtac ggccttccta cccggggaga tgaccttcct accccgggta gaacgatccg
    14341 ctgccggggt acggcgatca tctccatggg tacggcggcc ggggcgccct ccttttccac
    14401 ggacgccccg gccctgctct ctgtctggat ggggatgtcc gtttttccta ccccgccgct
    14461 ccctccgccg ccatccgttc ctcgagaatc gccccacacc gctcccaagc gacgccgccc
    14521 gccgcctcca gcagccaggc gagagattcc gacgcctgga gaaggtggac cccgcgctcc
    14581 ttcccccacg tacgtgacac caagacccgc ttcccctgtc tcgaaagatg gcgcggctgt
    14641 cgcgaatccc tcttcactcg cgacgaaacc ctctggattc gcgacggcgg aggcttcatt
    14701 cgagacgaga cgagacaaat tcgaaacggg acgaggcgga gtcgattcga atccagcggc
    14761 attcgattcg aatacgtggt gcgtcgtcgc gattgccgct tcggtggagc cgtctcttcg
    14821 agacgccgtc gcgtatgtcg ctccgttcgt cgcgaatgcc gcctgattcg agacggcacc
    14881 cggaggcgcc gtcgcgagtc gaggtcgagt cgcgacggat caggcgggtt ctgtttcgta
    14941 tctccagacg gcgtctcgag ctgtctggag ccacgtcgcg tatctcccgg gattcgagac
    15001 ggtgctcgcg ggattaaggg cgggtgagaa aggccggccg gtggcgctcc aaggtggaga
    15061 gatcccccgt ccctccgttg taagctcctg tcttcaggcc acttggccgg agaggatagg
    15121 gcccgatcca tggactacct gtcgttccag cccgatttcg agtcacccga cgccgtcgcc
    15181 gcctacgacg agctgccgtt gtggtcggcg atgttcgggc tgctcctgct gcggcacgtg
    15241 ccgctccggc ggggaatctc cgtgctggac gtgggatgtg gcaccggttt cccgctgctg
    15301 gagctggcgc agcgcctggg gccgacgtgc agggtccacg gcctggaccc ctgggagccc
    15361 gctctggagc gggcgcgctt caaggcccgg gtctggaacg tccgcaacgt cgagatccac
    15421 caggggaccg ccgaggccat gccgttcccc gatgccacct tcgagctcct cgtctccaac
    15481 ctgggcgtca acaacttcga ccggcccgaa gctgccctcg ccgagtgccg gcgcgtcgcc
    15541 aagcccgatg cccggctggt gctcaccacc aacctgcggg ggcacatgga ggagctgtat
    15601 caggtctacg cggagaccct gcgccagctc ggacgcgagg cggacctgcc caccctggag
    15661 cggcacgtgg accacagggc ctccgtggag agcgtcacca cgcttctgga gacccacggc
    15721 ttccgggtcg tccacgtcca ccgcgagacg gagacgatgc ggttcctcga cggcagcgcc
    15781 ctgctgcgcc actacttcat tcagctcggc ttcctgggcg gctggaaggg cgtggtggct
    15841 cccagcgatc aggccgaggt cttcaaggca ctggaggaga acctcaaccg ccgggccgag
    15901 gctcgcggcg ggctgggcct gacgatcccc atggtctgtg tcgaggcgga gcgggggtga
    15961 agggctctcc tggagggacg gatgcttgcg gctacaatga ccagcaaagg ccagatcacg
    16021 attccaaagg agattcgggt ggccctgggc ttacgggcag gatctcggct tttccttagg
    16081 atgagagaag acggagttgt agagatctct tcggaaaccg tggacctcat gtccctctgc
    16141 ggcatcctca aaccggcggt caggggtgtc acgctggaga acctggaagc ggctatccgg
    16201 gatggagcag caggtgagga atccgagagc ggcaactcaa tgaagcaagt ctcgaaaggg
    16261 gcgcccccac ctcggtcctc cccctgatcc aaccctctcc ctcccatggg gaggacgccc
    16321 ctccctcgga gtcacgctgt ggggagcaac tcgtcaaagg tcgtataaac cggatgcccc
    16381 cccgcctccg tcgggcggtt cccggggcga atgaggagcc ccgtccgcat gccggcctcg
    16441 gcggcggcgt cgagctcgtc caccacgtcg ctcaggaaga ggatgtccgc ggcggggagg
    16501 ccgtaggctt ccgcgatggc ggtgtaggcg cgggcttcct tcttggggcc ggtggtggtg
    16561 tcgtggtagc cctcgaagag gcgggtgagg tcgccgtagg cagtgtgggc gaagaggagc
    16621 ttctgggcgt ggacgctccc cgaggagaag acgcgcaggc ggaggccggc gtccttccag
    16681 gcttccaggg cgacggggac gtcggggaag acgacgctcc gcagctcgcc ggtgcggtag
    16741 ccctcgtccc agatgatccc ctggaggagc ttcaggcccg tggacttgcg gtcctgggcc
    16801 atgaggtggc gggcgtaggg ggcgccgtcg ccaaagtctg gcgtctccgc gctccgctcg
    16861 cggtcctccc ggtgctcctg ccgcagcctc tccagcgctt cggcgagctc cggagtctgg
    16921 tccggacggc cgcagtaggc gtccagcctc gcctcggcgt aggggaagag gacgtcgtag
    16981 acgaacgaga tgggggtggt cgtcccttcg atgtccgtca ggacggcgcg caccccctcc
    17041 gggagagcca cgcctacagc gtccgcgccg gcggcgggaa gtagttgggc ccgaagcaga
    17101 gcggctggaa gttggtgtcc acgccggtct cggtgtagtg cggcgtccag cccgacgggt
    17161 cctggaacag gcggatggcc cggatggtct tgtcggcgca gagatcgaac cagtggtgcg
    17221 tcccgcgcgg cacgcggatc atgtcgccct tgaccacctc gaggccgaac acctcgcctt
    17281 cgggaggatg gatgtggaac acgccgcggc cgtggacgat gaagcggacc tcgtcctcgt
    17341 cgtgccagtg ctccttgctg aacttgttga gcatggtctc gagaccgggc gtgctcggct
    17401 tgacgttgat gacgtcggcc gtcacgtagc cgccctcggc catcaggcgc tggatgggct
    17461 cgcggtaggc gtccaggatc tcctggtccg ccgcgtcctc cgggagctgg tccgagccct
    17521 cgaagcgccg gtaccagatc cccaccccgc tcaggatccc ggcgatctcg gccggatcct
    17581 ccactacacg cccgctctcg cggaaggtca acgtggccat cggatgctcc tcctgggacc
    17641 ctctgccatg gaggcgccgt gcccccgccg gtccccggga cagtgtccgc ctaatctagc
    17701 agaaaggtga accggcgccc caggaattcc tcggggaagg ccgtctatgt aggcagcctt
    17761 tcccgaggta tggggctcga ggtcccgacc cgggtccgat caaggaggag atgaccccat
    17821 ggagcacccg aagcgacccg ggctgttctg gcgccgcggc gacggcttgc acgtcttggc
    17881 actcggatcg ttagtcatgg taggggcgac ctgctgccac agcatggccg gcgtgccaac
    17941 gcagcacatc gtcatgttcg atagccatgg caaggccatg gaccccacgg ggaagatcga
    18001 ctgcgaggag ccggtgccgc ccaaggtcga agcccggacg gccgaccagc tcatgactcc
    18061 gggcgagccc gactggaacc gctgcaacgg caagatcagc cggctgtcgt ccttcaagca
    18121 gttggattca agggagttca gcaaatatct cgatcaggtg ttcagatcca tcccggccac
    18181 gccaggagcc ccttcgccgg tgagttcaac gacgggagtt actggcagga gagcttctga
    18241 cttccggaga aggaggtcag cgagagcgct cggcagcttt gcccggatga tccggaaacc
    18301 aggtaacagg ccgggaggcg gggaccgcgc cgcccttgcc cgggtccccg ccccctgcta
    18361 gagtcgccgg ctccatggcc cccccgtccg ccggctccga tcctcgtgag gtgctcatcg
    18421 cggcatcgcg gctcctctat ggacgggggt ggatggcggg cacggcgggg aacctgtcgg
    18481 tgcgggaggg ggaggggccg ttctggatca ccgccagcgg caaggccaag gggaccctgg
    18541 gacgggcgga cttcgtgcgg gtctcgctcg atggcgaagt ggtggagcgg ggcggaccgg
    18601 acagccggcc ttcggcggag accagcatcc atggcgcggc ctaccggctg ttccccgagg
    18661 cgcgcgcctg ctaccacgtg cacacgatcc ccaccaacct ggtgacccgt ttcaccgagg
    18721 gcgacgagct gccgctgccg ccgatcgaga tgatcaaggg gctgggcgtc tgggagcagg
    18781 agccgcgggt gagcgtcgcc gtgctgccca accacctgga cgtgccccgg atcgcggcgg
    18841 agatggaggc ccggttccgg gccgcggccc ccaggctgcc ggggttcctg atccgcgatc
    18901 acgggctcac cgtctgggcc cagagcccgg aggcggccct caactacctg gagctcttcg
    18961 actacatctt cggctgcatg gtggccgccc gctcgtcgga cctacgttgg tagctcattc
    19021 tccgcgactt cctgcactct tatatagacg ggtacgggcg agggggcctc gatctccctt
    19081 ccgtgccact cccgagccgg atccgcaaca gggcgacgaa aacagatgac gctggaccct
    19141 tcccattccg ctcggtggcc cgaggagctg tagccatggt cgatatgacg tcccaggccg
    19201 gggagctctc ccccaagcag cggcgtctgc tggagctgat gctcaagcag aagcagaagg
    19261 agaaggaagc cggagcccag acccccgcgg ccctgccgcg ccggcagccg tccgacccgc
    19321 tggtgctctc cttcggccag aagcgcctct ggttcattga gcagctccag cccggcagcc
    19381 cggcctacca cgtgcccggc gccgtgcgca tccgcgggcc gctgaacgtg ccagccctgg
    19441 aggccgccct ggccgaggtc gcccgccgtc acgagatcct gcgcacccgc ttccacgtgg
    19501 tggaggggca gccggctctc gacatcgagc cggagcccgc cctggcggtg gaggtcgtcg
    19561 acctgcggcc ggtgcccgcg gaacggcgcg aggaggagct cgcttatcag gtggacgccc
    19621 gggtgctcca gcccttcgac ctgtcccgga cgccgctcgc ccgtacggtc ctctaccggc
    19681 tgggggacga ggagcatgtc ttcctcctga tcctccagca catcatctgc gacatctggt
    19741 cggtgggggt attcttccgc aacaccatgg cgctgtacga cgccttcctg gccggcagcc
    19801 cgtcgccgct ccccgagctg ccgctgcagt acaccgacta cgccctctgg caggagcgcc
    19861 tgctccaggg ggagggcctg gagcgcctcc tctcctactg gaagggggag ctcgccggcg
    19921 cgccgttcgt catcgacctg cccaccgacc acccccggcc ggcgctccag agcttcgagg
    19981 ggagccgccg ctacatgaac atgagcgtcg agctgaccga ggcgctcaag gccgtggcca
    20041 aggcgcagga cgccagcctc tacatgatcc tgctggccgc cctctatgcc ctgctgcacc
    20101 gctacacggg ccaggagtcg atcctcgtcg gcgtgccgat ggcgaaccgc ggccgggtgg
    20161 agctggaggg gctggtcggg ctcctcttca actccctggt cctgcgcgcc gacctcgggc
    20221 cggagatgcc gttccgcgat ctcctctccc aggtgcggga gcggaccctc ggcgccttca
    20281 accaccagga cctgccgttc gagaagctgg tcgaggagct gcgggtcgag cgcgacatga
    20341 gccgcaaccc catctaccag gtcctcttcg ccttccagaa cgtgccgccg tccaccatgg
    20401 cggcgcgcga cctggcgatc cagcgctacg aggtgcggga gaccgcctcg cgcgaggacc
    20461 tggagctgga catgcgggag acccccgagg ggctcgccgg ctggttcggc tacgaccggg
    20521 cgctcttcga cgcctccacg gtgacccgca ccaccctcca cttccagaac ctgctggccg
    20581 gcatcgccgc cgaccccgcc cggccgctgg ccgagttgcc gctcctcacc gcttccgaga
    20641 cccaggccct gctcttcgag ggcaacgata ccggggtgac cgacacggcg gaggagagct
    20701 tcgaggcgct gttcgaggca cgggtggcgg agacgccgtc cgctcccgcc gcggtcttcg
    20761 gcggcacgcc cgagacctat gccgagctga gcgcccgcgc caaccgcctg gccaggcacc
    20821 tggtggggat ggggatcggg cccggggtgg tcgtcggcct gctggaccgc cgcggccccg
    20881 acctcctggc cgccatcctg ggggtcgaga aggcgggcgg tgcctggctg ccgctggacc
    20941 cagagcaccc ggcccggcgt cacgctcagg tgctcgccgc cagcgccacg ccgctggttc
    21001 tggcccacga ggacctgctg ccggtcctca ccgaagcggt ggccgagctg ccggaggggc
    21061 gccatccgcg gctcgaggtc ctggcggacc tcctggcccg cccgggcgac gagagcacgc
    21121 cgccgcgccg cgccgcgccg gacgacctgg cctacaccct cttcacctcg ggatcgacgg
    21181 gcgtgccgaa aggggccatg gtcacccgcc ggggcatgct caaccacctg cgggccaaga
    21241 tcgaggatct gggcctcggc ccgtccgacg cggtggcgca gaccgcctcc cagtgcttcg
    21301 acatctccgt ctggcagttc ctcgccgccc tggccgtggg cgggcgcgtc catatctatg
    21361 aagatgagac cgcgcacgac ccggcccgcc tgctcgcgcg ggtggagacc gatggcgtca
    21421 ccgtggtcga gaccgtcccc tccctgctgc gcctgatgct ggaggaggcg gagcggagcg
    21481 gcggccagtc ccggccgggg gcgctgcgct ggatgatccc cacgggcgag gcgctccccc
    21541 cggagctggt ggaccgctgg ctccggcgct tccacgcgat ccccctggtc aacgcctacg
    21601 gcccgaccga gtgctcggac gacgtcaccc accagcgcct ggacgggccg ctgggcgacg
    21661 gcacggcgcg ggtgccgatc ggtacgccga tccgcaacac ccgcctctat gtcgtggatc
    21721 gcggcctgcg cctggtcccc atgggggtgg cgggcgagct gtgcgtcggc ggcgaggggg
    21781 tggggcgcgg ctacctgggg gcgccggaca agaccgcggc ggtcttcgtt cccgatccct
    21841 tcgctgggcc gtctggtcag gacggagccc ggctctaccg caccggcgac ctggcgcggc
    21901 ggctcgccgg cggcgccttc gacttcctgg ggcgcatcga ccatcaggtc aaggtgcggg
    21961 gcttccgcat cgagctgggg gagatcgaat cggtcctcgc ccagcacccg gcggtgcgcc
    22021 aggccgtggt cctggcgcga gaggccgggg cgtcggagcg ggcgctcgta gcctatgtcg
    22081 tcgcccaggc cggcgaggag accgagcgcg agctgcgcga tttcctccag acgtggctgc
    22141 cggactacat ggtgccggcg gcgttcgtga cgctggaggc gctgccgctc accccgaacg
    22201 gcaaggtcga ccggcgggcg ctgctggcgc tgggcggtcc ggagct
//
LOCUS       DASPJI010000296        17993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_77412, whole genome shotgun sequence.
ACCESSION   DASPJI010000296 DASPJI010000000
VERSION     DASPJI010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17993)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17993
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77412"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4124
                     /locus_tag="VEL74_07875"
     CDS             <1..4124
                     /locus_tag="VEL74_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62485.1"
                     /translation="FDAPTVAGLAAFLEESQPEAVAHLIAETRAVEAPRRLAPVERRD
                     WSRETPPLSFVQERLWFLHLLDPEGGVYNMPNAFRLRGPLEPAVLDRSLCEVVRRHET
                     LRTRFESRDGLPMPVVEPAPRSILPVADLSALPGAPRDEEARRLFTVEASRPFDLARG
                     PVVRSLLLRLAPDEHGVLFVAHHIACDGWSMGLWAREVEALYTAFAAGEPSPLPEPPI
                     QYADFAHWQRSWLAGEELAAGVEHWRERLQGAPPYLDLPTDRPRPPIQTFRGEWETAA
                     LPAGLAAELRSLGQSHNASLFMVMLAAFQLLLSRLSGQEDVLVGSPIAGRVPEAEGLI
                     GCFLNTLVLRTDLSGAPTFAGLLGRVRETALEAFAHQDVPYEKLLEVLRPERDLSRNS
                     LFQVLFNMLNAPTAEVRLPGVGIEGEGLAEASSKFDLTLYVAEQNDELLLNLVYNADL
                     FDRARIQEMLRQYRSLLAQAVAAPDAAVAGYSLLTPEAAAVLPDPAADLGGRWEGTVH
                     GLFAEWAERQPERVALADRNGEWTYGELDQAVQRLAARLLAGGIEPGDRVAIHAHRSA
                     ALAWALLGVLKAGAAFVILDPIYPAARLVEVLRLARPTGWLRLEGAGPLAEPLATFVA
                     EAPFRVRFDIPAGGPETAASAVGYPPLPDTWPSLGPDDVASHAFTSGSTGVPKGIVQT
                     HGSMSFFLPWHRDVLGYGEADRHTLLSGLAHDPLQRDVFYALGSGAALCVPDPDRIGE
                     PGYLAEWMRRERVTVTNLTPAMAQLVTEGEHAALPYLRCVVLAGDILTRLDVDRVRRL
                     APQARLVNVYGSTESHRALSYHLITEADEWGKQVLPLGRGKNGCQLLVLTAAGMRAGV
                     GELGEVAIRSRHLAAGYLGDDALTRDRFVANPFAQDPADRLYRTGDLGRYRPDGEVEA
                     AGRADQQVKVRGYRVEPGEIEAELGRIPGVREAVVVGRDDSQGSKRLVAYVVLDSADA
                     GEAELRELLRERLPSYMVPAVFVALGELPLNPNGKVDRRALPEPPSQAGTALERRSVP
                     PRDELESRLARIWEEVLGTAPIGVTDGFFDLGGHSLLAVRLTAWIARELGQTLPLAAL
                     FQAPTVEQMAVLLRRREPMADGTLVPLRTGGSRPPLFLVHPAGGQVFSYTGLVRGLGP
                     GQPVYGLQDTAPPESPRTLAGLASRYLKEIRAVQPHGPYLLAGWSFGGRVAFEMARQL
                     DAAGEEAALVAMLDTGLVQPPATPLRDAELLAEVVGELPLSIDELRRADDPLAALVET
                     ARETGALSPDITLESARHLLDVFKAHLEVARTDRPRPYRGRVVFFAAEVQPEEIAADP
                     SHGWSRLAGELELRRVPGDHVTMIHNAHNVRVVSERLAESIEEALAEVEAVVG"
     gene            4923..6110
                     /locus_tag="VEL74_07880"
     CDS             4923..6110
                     /locus_tag="VEL74_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HYG62486.1"
                     /translation="MTRWKWPQILGIAVLMLPAASGAVPIRVTDGLVGEWHLQTTRLL
                     SLELAPDTSGYGRTGRVYGGTGSSYGWMYTGVNLTGDKYVQVANSSNLNFGTGSFTLA
                     AWIKMSDTSRSVKTIIDNRGSDGRGYAFAVHGGHQLLLQMADETGWVNLLSEPNWLSP
                     NRWHHVAVSVARTTWPVNATFYVDGYRAGIVTPKMGTINNTNLPFLIGGHKDSASSRF
                     SDRIDEVLVYNRSLPIWEIWSIMNPGRPLYEPSYWNSGSRLANNNCYNYANNKPTDTF
                     AQPGRAAGAQWTSLTCAAVHQAAVADGLQPISGYPSYVLDLKTGVALVVAPGIDYHWY
                     RLDDDGTWTHKPGNSPATNRDNSGNIITDPRTANRGIYTEFCGFYTLWSDITQGWGHE
                     QIN"
     gene            6140..6679
                     /locus_tag="VEL74_07885"
     CDS             6140..6679
                     /locus_tag="VEL74_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62487.1"
                     /translation="MSRFSFKHFLGVVPGLALVGLLAAVPSSAQDAKPQPEKGFRVTY
                     LLFSGRPNPSVVVTEPGQVAEIAQQLAGTLAGGARRDAAASHPVLGYNGIMIQRLGAS
                     KGEGWFVVNRDTLRVEGGDAQVPASETGGAKSLAAFSRSTLAVSREAEEIEDLLISLG
                     MNAGVIDEAGQSVLYSPPE"
     gene            complement(6763..7221)
                     /locus_tag="VEL74_07890"
     CDS             complement(6763..7221)
                     /locus_tag="VEL74_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62488.1"
                     /translation="MAEQDEPPTAGEADVAGPWRIEAIPGEGFGLFRIGESLGRGFSP
                     LAVFPSQWLALIAAAVLPGTGRDPLLRIDKQPDADGAYPLLLPGLGGEEVAGRLRVFD
                     EPLVDAINLVILLLQVPESLAWMLEAAGTVALERCGAILDERLPETKAAG"
     gene            7789..8145
                     /locus_tag="VEL74_07895"
     CDS             7789..8145
                     /locus_tag="VEL74_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG62489.1"
                     /translation="MLVEDDVETRLAYRALLEHAGWNVHEAADGEQALRLVDDLLPRA
                     AIIDISIPGMDGWETTRRLKLDERTRDVPVLLVTGHALDEDRQRAQDLGCAAYLVKPV
                     LPSRFVEEVARLVEKP"
     gene            8226..9005
                     /locus_tag="VEL74_07900"
     CDS             8226..9005
                     /locus_tag="VEL74_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG62490.1"
                     /translation="MLVVDADGKVAAQLATALEGSPERERFELARASGLTETLEKLAA
                     GDTDVVLLPISPLDQGLGPLVTIRAEAPNLPVVVLCATGDEPLAVKAVQLGASDYLVA
                     ERLYGTLAARSLRHAAEVARMRAVLAAHHAEWPASLGERASSRPAPLRAAFPERFAEL
                     VRDYGEILDRAVEQILQRQLAPFEHRLQKLASVAADLRAGPRDIVELHATAMKQREAE
                     QGPQRMKLYLAEGRLRLLELMGYLVAVYRNRWLGGPRSGAD"
     gene            9033..9338
                     /gene="kaiB"
                     /locus_tag="VEL74_07905"
     CDS             9033..9338
                     /gene="kaiB"
                     /locus_tag="VEL74_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015142658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiB"
                     /protein_id="HYG62491.1"
                     /translation="MDRYVLKLYISGRSPRAERAIANLRRLCKDELEGCYELEIIDVL
                     EHPQLAEDDRILATPTLIKQLPPPLRRVIGDLSNHDKVLLGLDVRPRDERPREGEKP"
     gene            9352..10863
                     /gene="kaiC"
                     /locus_tag="VEL74_07910"
     CDS             9352..10863
                     /gene="kaiC"
                     /locus_tag="VEL74_07910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HYG62492.1"
                     /translation="MSERVLPPAKVLTGIPGFDNLTYGGLPANRSTLVSGSSGSGKTI
                     FAVQYLARGITELGEPGVFVTFEESPDDIRRNVASFGWDLAAWEEDGKWAFVDASPDP
                     EEEILEAGDYDLGGVMARIKHAVARTGARRLVVDSLSAVFSQFQDASVVRNELFRIAR
                     AARKMGVTAVLTAERTEEYGPVGRYGVEEFIADNVVVFRNVLEEEQRRRTLELLKFRG
                     TDHQKGEHPFVIEADRGLVVTPLTGVPLEQRSSNVRVTSGNPELDAMCGDGFFRDSVV
                     LVSGATGTGKTLTVTHFVAGGAREGDRGLLFGYEESREQLVRNAAGWGVDFGSMEDAG
                     MLRVHCEYPEARGLQDHLLRIKRMIEEFAPQRVVIDSLSALERVGPPKAFRQMVIDLT
                     SFIKQKSITGLFTNTTPTLVGGTSVTEAHISSLTDSIILLRYVEMFGEMQRGLMVLKM
                     RGSPHDKEIRQVTIDATGMHLGEPFRSVSGILSGNPVHSREELARLEGLFPQE"
     gene            10933..13389
                     /locus_tag="VEL74_07915"
     CDS             10933..13389
                     /locus_tag="VEL74_07915"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYG62493.1"
                     /translation="MSGKGLKVKKVAISPPDFRVLFESAPGLYLVLTPDLRIIAASDA
                     YLQATMTKREEILGRDLFEVFPDNPEDTAASGTRNLRASLERVLATKKPDAMAVQKYD
                     IRRPEEDSGGFEERYWSPVNSPVFATNSEIAYIIHRVEDVTDFVRLRQQGIEQTRRTE
                     ALQSEAERMEAEIFLRAQQLQEANAQLREVNAQIEQRDTERERIIEGTGDAMVVVDLE
                     GSVRFANSAAEALFGQRREKLLGSPFGFPVVSGETTELDVAGSRVAEMRVVGLLWEGR
                     SAYLASLRDITGRKEAEDAERRLWRERTAREESEKERRRLQELLERAPAAVLTTRGQD
                     HVCVFANPRATELVGGRELLGRPLAETLRELRGQGFLEAFDRAFQDVASGASPELELS
                     LAGERGEAGEAWRCVDVTWVPLGQDGRVDGVMCFAHDVTEQVAMRRALEQTMERLREE
                     ERRKDQFMAVLGHELRNPLAGIDGGLRLLAGGADPDREQWALAMMRNQVRQLTSLLDE
                     LLDVSSIARGKLELRRRVVPLTQIVEAATASTAARLSDFEQRLIVSMPDEPLHMDADP
                     RRIEQVLANLLVNASKYSDRGTEIRLEVRSEAEEAIIEVSDQGFGIDPQMIERIFEPF
                     IQAPRGDGGKMAGGLGIGLTLVRQLAELHGGSAVAHSDGLGRGSTFTIRLPIAAEAAP
                     EVPAESTATVSLAEHRILVVDDNVNAATALTELLKLRGCNARAAISGGEALTAASEEV
                     FDVILLDLDLPDIVGYEVATELRRRPAGCRLLIIAVSGFGDEQARERSRKSGIDHHMV
                     KPVEIDELLRVIASGTLVTR"
     gene            complement(13855..14739)
                     /locus_tag="VEL74_07920"
     CDS             complement(13855..14739)
                     /locus_tag="VEL74_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYG62494.1"
                     /translation="MTCRTVQSRLLLTPRAHVQEIVLGVLARAKRMYPLDLIVFAFAS
                     NHFHLLIWVEDAGQMSAFMGYLNGNLARELGRLADWKERFWGRRYQSIPVSDEEGAQI
                     ARLKYILSHGAKEGLVADPREWPGAHAVHALLSGEPVVGVWYDRTQEYRYRLRGKEPE
                     PRQFATEEVLTLAPLPCWRHLPPEEYRRRMVDLVAEIVEEARVVREAKGTEPLGADMV
                     RRQQPHTRPNRTKKSPAPLFHTASKRAYLALREAYSRFVAAFRDASEKLRAGDRLVEF
                     PEGSFPPALPFVRRATAG"
     gene            14921..16078
                     /locus_tag="VEL74_07925"
     CDS             14921..16078
                     /locus_tag="VEL74_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HYG62495.1"
                     /translation="MSQTNERTSQAPHGGPADSEHDQKVPEIDLLSPLTLRGVTLRNR
                     IVMSPMCQYSAEEGLANDWHLVHLGSRAVGGVALIIVEATAVTREGRISPRDLGIWGD
                     EHVEPLARIARFVHSQGAVPGIQLAHAGRKASCEPPWLGGAPIPADRPDGWTVVAPSA
                     VPFHEAGAVPVPLDEAGIDGVVAAFEAAARRALEAGFRVIEIHAAHGYLLHEFLSPLS
                     NQRDDHYGGSLENRMRLLLRVAGRLRALMPEELPLFVRISATDWVEGGWDVEQSIELA
                     KRLREAGVDLIDVSSGGTTPRARIPVGKGYQVPAARRIRDEADVRTGAVGLITEASYA
                     DEIITGGDADLVLLGRELLREPYWALKAQHEMDEEPAWPIQYGYAVKRRSR"
     gene            16592..>17993
                     /locus_tag="VEL74_07930"
     CDS             16592..>17993
                     /locus_tag="VEL74_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYG62496.1"
                     /translation="MTDRPAGDHLQGETIGPYRLDAPIGSGGMGTVWRAWDERLRRHV
                     AVKRVLRDSEVRRERLRREAQAVARLSHPAIVHVYDLLEGDGADWIVMELVSGEDLRK
                     ILDRTARLDVGQALHLGRQIAEGLAEAHAQGILHRDLKAANVMVTASGRAKILDFGLA
                     KTLRGEGSGAADVDQSLSLPGTVLGTCYAMSPEQALGHDLDPRSDLFSLGSLLYEMLT
                     GSAPFRAEDARSSLARVLYFQPRPLREVRPEVPSGLSSLIERLLEKEPAKRPAGAGEV
                     ARILATLATTSLESSNSSGAGGKVAASTANSILETIVERLPVAEVPAPIKPPAGEGRR
                     DPSSGVGGERRTVTVVCCSVVSAEAAVPLELELLSASMSSIESLAQEVCRDLAGHLGA
                     VLSHMVWIYFGYPVSHEDDAVRAVRAARELMVRAREAGLLRRGLALRFGIHTGPAVVW
                     LRPGQADRLQLGSTFDF"
BASE COUNT         2873 a         6042 c         6175 g         2903 t
ORIGIN      
        1 tgttcgacgc cccgaccgtg gccgggctcg ccgccttcct cgaagagagc cagcccgagg
       61 ccgtggccca tctcatcgcc gagactcggg cggtggaggc cccgcgccgt ctcgcgccgg
      121 tcgagcgccg ggactggagc cgggagacgc cgccgctctc cttcgtccag gagcggctct
      181 ggttcctgca cctcctcgat ccggaggggg gcgtctacaa catgcccaac gccttccggc
      241 tccgtggacc cctggagccg gcggtcctcg accggtccct gtgcgaggtg gtgcggcgcc
      301 acgagacgct ccgcacccgc ttcgagtcgc gggacggact accgatgccg gtggtggagc
      361 cggcgccgcg gtcgatcctc cccgtggccg acctctcggc gctgccgggc gcgccgcggg
      421 acgaggaggc ccggcgcctc ttcaccgtcg aggcctcgcg tccgttcgac ctcgcccgcg
      481 gcccggtggt gcggagcctg ctgctccgtc tggcgccgga cgagcacggc gtcctcttcg
      541 tcgcccacca catcgcctgc gacggctggt cgatggggct ctgggcgcgg gaggtggagg
      601 ccctgtacac ggccttcgca gcgggggagc cctcgccgct gcccgagccg ccgatccagt
      661 acgccgactt cgcccactgg cagcggagct ggctcgccgg cgaggagctg gcggcgggtg
      721 tcgagcactg gcgcgagcgc ctgcaagggg cgccgcccta cctcgacctg cccaccgacc
      781 ggccgcggcc gccgatccag acgttccgcg gcgagtggga gacggcggcg ctgccggccg
      841 gcctggcggc ggagctgcgg tccctgggcc aaagccacaa cgcgtccctc ttcatggtga
      901 tgctggcggc cttccagctc ctgctctcgc ggctctccgg ccaggaggac gtgctcgtcg
      961 gctcgcccat cgccgggcgg gtgccggagg ccgaggggct gatcggctgc ttcctgaaca
     1021 cgctcgtgct gcgcaccgat ctctcgggcg cgccgacctt cgccgggctc ctcggccgag
     1081 tgcgcgagac cgctctggag gcgttcgccc accaggacgt gccctatgag aagctcctgg
     1141 aggtgctgcg ccccgagcgc gacctgtccc gcaactcgct gttccaggtg ctgttcaaca
     1201 tgctgaacgc ccccacggcc gaggtccggc tgccgggggt gggcatcgag ggggaggggc
     1261 tcgcggaggc ctcctccaag ttcgacctca ccctctacgt ggcggagcag aacgacgagc
     1321 tgctgctcaa cctggtctac aacgccgatc tcttcgaccg cgcgcgcatc caggagatgc
     1381 tgcggcagta ccggagcctc ctcgcccagg ccgtggcggc cccggacgcc gccgtcgccg
     1441 gctactcgct gctcaccccc gaggccgcgg ccgtgctgcc cgaccccgcg gcggacctcg
     1501 gtggccgctg ggagggcacg gtccacggcc tcttcgccga gtgggcggag cgccagccgg
     1561 agcgggtggc gctggccgac aggaatgggg agtggaccta tggcgagctg gaccaggccg
     1621 tccagcgcct cgccgcacgc ctcctggccg gcggcatcga gcccggcgac cgggtggcga
     1681 tccatgccca ccgcagcgcc gcgctcgcgt gggccctgct gggggtgctc aaggccgggg
     1741 ccgccttcgt catcctcgac ccgatctatc ccgccgcccg tctggtggag gtcctgcgcc
     1801 tcgcccggcc cacgggctgg ctgcggctgg agggggccgg cccgctggcc gagccgctgg
     1861 ccaccttcgt ggcggaggcg ccgttccgcg tccggttcga catcccggcc gggggacccg
     1921 aaaccgccgc gtcggccgtg ggctatccgc cgctgccgga cacctggccg tccctggggc
     1981 cggacgacgt ggcgagccac gccttcacct cgggctcgac cggcgtcccc aaggggatcg
     2041 tccagacgca cggctccatg tccttcttcc tcccctggca ccgcgacgtg ctggggtacg
     2101 gcgaggccga ccgccacacc ctgctctccg gcctggcgca cgacccgctc cagcgcgacg
     2161 tcttctacgc gctcggctcg ggggcggcgc tctgcgtccc ggacccggac cgtatcggcg
     2221 agcccggcta cctggcggag tggatgcgcc gcgagcgggt gacggtcacc aacctgacgc
     2281 ctgccatggc acagctcgtg accgaggggg agcacgccgc gctgccgtac ctgcggtgcg
     2341 tggtcctggc gggcgacatc ctcacccggc tggacgtgga ccgcgtccgc cgtctcgccc
     2401 cccaggcgcg gctggtcaac gtctacggct ccacggagtc gcaccgggcg ctgagctatc
     2461 acctgatcac cgaggcggac gagtggggca agcaggtgct ccctctcggc cgcggcaaga
     2521 acggctgcca gcttctggtg ctcaccgccg cggggatgcg cgccggcgtc ggcgagctgg
     2581 gcgaggtggc gatccgcagc cgtcacctgg cggcgggcta cctgggggac gatgccctca
     2641 cccgtgatcg cttcgtggcc aatcccttcg cccaggatcc ggcggaccgc ctctaccgca
     2701 cgggcgacct cggccgctac cggccggacg gcgaggtgga ggccgccggc cgggccgacc
     2761 agcaggtgaa ggtccgtggc taccgcgtgg agccggggga gatcgaggcc gagctcggga
     2821 ggatccccgg cgtccgcgaa gccgtggtgg taggccgcga tgactcccag gggagcaagc
     2881 gcctggtggc ctacgtggtg ctggacagtg ccgacgccgg tgaggcggag ctgcgggagc
     2941 tgctgcgcga gcgcctgccc tcctacatgg tgccggcggt cttcgtggcg ctcggcgagc
     3001 tgccgctgaa ccccaacggc aaggtggacc gccgcgccct gcccgagccg ccctcccagg
     3061 cgggcaccgc cctggagcgg cggagcgtgc cgccccggga cgagctggag agccggctcg
     3121 cccggatctg ggaggaggtg ctgggcaccg cgcccatcgg cgtcaccgac ggcttcttcg
     3181 acctgggagg ccactcgctg ctggccgtcc gcctcaccgc ctggattgcc cgggagctgg
     3241 ggcagacgct ccccctggcg gccctcttcc aggcgccgac ggtggagcag atggccgtcc
     3301 tcctgcgccg ccgggagccg atggcggacg gcacgctggt gccgctccgt accggaggga
     3361 gccggccgcc gctcttcctg gtccatccgg ccggcgggca ggtcttctcc tacaccgggc
     3421 tggtgcgcgg cctcggcccc ggccagccgg tctacggcct ccaggacacc gcgcccccgg
     3481 agtccccgcg gaccctggcc ggtctcgcgt cccgctatct gaaggagatc cgtgccgtcc
     3541 agccgcacgg gccctatctc ctggccggct ggtccttcgg tggccgtgtc gccttcgaga
     3601 tggcccgcca gctcgacgcc gccggcgagg aggccgccct ggtggccatg ctcgacaccg
     3661 gcctcgtcca gcctccggcg accccgttgc gcgacgccga gctgctggcc gaggtggtgg
     3721 gggagctgcc cctctcgatc gacgagctgc ggcgtgcgga cgacccgctg gccgccctgg
     3781 tcgaaacggc cagggagacc ggagccctgt cgccggacat caccctggag tcggcgcgcc
     3841 atctcctgga cgtcttcaag gcccacctgg aggtggcgcg caccgaccgg ccgcggccgt
     3901 accgggggag ggtggtgttc ttcgccgccg aggtccagcc ggaggagatc gccgccgatc
     3961 cgtcgcacgg ctggtcgcgc ctggccggcg agctggagct gcggcgggtc cctggggatc
     4021 acgtgaccat gatccacaac gcccacaacg tgcgcgtggt gtcggagcgg ctcgccgagt
     4081 cgatcgagga ggcgctggcg gaggtggagg ccgtcgtggg ctagcccgct gaccgtggaa
     4141 cacaaaagag tgttcccgga acacaaaccg gccctcctgg aacacaacgg agccttcctg
     4201 gaacactaac ccccggcggt ggatcattcc cgcgccgggg aggatcattc tccccgcggc
     4261 gggcgtcatt cgctgtgcct ccgggatcat tccccggtgt tcccggatca ctcccgggac
     4321 tctgtttgtg ttccgggaac gggggagagt cactctccct gccccccgga tcactcccgt
     4381 gagtcgggga atgatctccc cgccctcccg aatgtgcttt tcctcggact ttgtgatctc
     4441 tggcgcttcc cggacggtcg tcgggcacgg ggagctgtcc gcgacaggcc gagtagaagt
     4501 cctaccacgt cccccgagcg cgctgtgccc cgccccagcg ccgcgctcgg gggacatgtc
     4561 catcgattgt caaagaaccg actgaacagc ccgcggctcg gagaaccggg agcaggctgc
     4621 gcagtctatc ctcttctctg tagactctga tgtcaatcgc gggtatgcta tgcaggaggc
     4681 aggggcgaca gctttcctgc cctcgggttc attgacaact gaatcgctag gggcgccgcc
     4741 ccacggctgc gcccttggag catccgtgcc ctcgcggagg aggcggagga gcagtcctcc
     4801 gcctcccgag accggccggc ggctcttccg gaaggctcgc ctttcctccg ccgaagcacc
     4861 ctccctttcg atgccgactt atgttcatgt atctatgttc ggaccatcga aaggagtgtg
     4921 agatgacgag atggaaatgg ccgcagatcc tggggattgc ggtgttgatg ctgcccgcgg
     4981 cgagcggggc cgtgccaatt cgggtcaccg acggtctggt cggcgagtgg cacctgcaga
     5041 cgacccgcct gctgtcgctg gagctggcgc ccgacaccag cggctacggc cggacgggcc
     5101 gggtctacgg cggcacaggg tcgtcctacg gctggatgta cacgggagtc aacctcacgg
     5161 gcgacaagta cgtccaggtg gccaactcgt cgaacctgaa cttcggcact ggcagcttca
     5221 ccctggcggc ctggatcaag atgtccgaca ccagccggag cgtcaagacc atcatcgaca
     5281 accgggggag cgacggccgc ggctatgcct tcgcggtcca cggaggccat cagctccttc
     5341 tgcagatggc tgacgagacc ggctgggtca acctgctttc cgagcccaac tggctctccc
     5401 ccaaccggtg gcaccatgtc gcggtcagcg tcgcgcggac gacgtggccg gtcaacgcca
     5461 ccttctacgt cgacggctat cgcgccggca tcgtgacgcc caagatgggg acgatcaaca
     5521 acaccaacct gccgttcctg atcggcgggc acaaggattc cgccagctcg cggttctccg
     5581 accggatcga cgaggtcctg gtctacaacc gctccctgcc gatctgggag atctggagca
     5641 tcatgaaccc gggtcggccg ctctatgagc cgagctactg gaacagcggg agccgcctgg
     5701 ccaacaacaa ctgctacaac tacgcgaaca acaagccgac cgacacgttc gcgcagcccg
     5761 gccgtgccgc gggcgcgcag tggacgtctc tgacgtgcgc cgccgtccat caggccgccg
     5821 tcgccgacgg gctgcagccg atctccggct atccgagcta tgttctggac ctgaagaccg
     5881 gcgttgccct ggtcgtcgcg cccggcatcg attatcactg gtaccgcctg gacgatgacg
     5941 gcacgtggac gcacaagccc ggcaactcgc ccgcgacgaa ccgcgacaac tcgggcaaca
     6001 tcatcaccga cccgcgcacg gcgaaccgcg gcatctacac ggagttctgc ggcttctaca
     6061 cgctgtggtc ggacatcacc cagggctggg gccacgaaca gatcaactag gccgacgcac
     6121 gagattcagg agaaaaccta tgagccgttt ttcgttcaaa catttcctgg gcgtcgtgcc
     6181 gggcctggcg ctcgttggcc tgctcgccgc cgtcccctcg tccgcgcagg acgcgaagcc
     6241 ccagcccgag aaggggttcc gcgtcacgta cctgctcttc tcgggccgcc ccaacccgag
     6301 cgtcgtcgtg acagagcccg gccaggtggc cgagatcgcg cagcagctcg ccggcacgct
     6361 ggccggcggc gcgcggcgcg atgccgccgc ttcgcatccg gtgctcggat acaacggcat
     6421 catgatccag cgcctcggag cttcgaaggg agagggctgg ttcgtcgtca acagggacac
     6481 gctgcgcgtg gaaggcggcg atgcgcaggt ccctgccagt gagaccggag gcgcgaaatc
     6541 cctggccgcg ttttcccggt cgaccctcgc ggtttcccgc gaggccgagg agatcgaaga
     6601 cctcctgatc tctctcggca tgaatgccgg ggtgatcgac gaggccggcc agtccgtgct
     6661 gtactccccc cccgagtgag ccgagacagg cactaccctt tccggagcaa cggggcgccg
     6721 ccggtgagct gcaagccggc ggcgcccatg tctgtaaggg tcctacccgg ccgccttcgt
     6781 ctccggcagc cgctcgtcga ggatggcgcc gcagcgctcg agcgccaccg tccccgccgc
     6841 ctccagcatc caggccagtg actccggaac ctggagcaga aggatcacta agttgatggc
     6901 gtccaccaga ggctcgtcga acacccggag ccgtccggcg acctcctcac ccccgagacc
     6961 ggggaggagg aggggatacg cgccgtccgc atcgggctgc ttgtcgatgc ggagcagcgg
     7021 gtcccgcccc gtgccgggga gcacggcagc ggcgataagg gcgagccact gggacgggaa
     7081 caccgccaag ggagagaatc cccgacccag gctctcgccg atccggaaca ggccgaagcc
     7141 ctcacccggg atcgcctcga tccgccaagg accggcgaca tcggcctcgc ccgccgtggg
     7201 cggctcgtcc tgctcggcca tccggcacag aaactgcgtt ttgaagacgt tggggtgtac
     7261 accagagctg ataggctgct cgtagccttg catatgtcct cccaatagga ctttgtaggg
     7321 ttaggcctgg gggagagttc gagctctctt ccgggccgta agccagcacc atgccagccg
     7381 tccctccttc cagagatgtt gtctggatct tcctgaaccg ttcttccgtt tcccctcgac
     7441 accccttctc ccgggaagac cgcacgccag gtttgtgtga ggcgatttag caaagcggta
     7501 acggggtgtc aagcaggtaa cgaagatctg cggggtcctg tcgtccttac cctgagaagg
     7561 gagtctcgcc ctgagaagag gtgaagccta tgggttgacc gcaggaactg tgtagcgtca
     7621 tctccttaac acgcaacgct cgactgatcg ctaccttctg tccctttgat agcctccatg
     7681 gcaccgcacc gttcgagagc caccgccccc gccgcctgag gcccaagatc ggatgctaca
     7741 ctccggtccg tgaggaccat catgggtagc ggagtgcgag ggccgatctt gctcgtcgag
     7801 gatgatgtgg agacccggct tgcctatcgg gcgctgctcg agcacgccgg ctggaatgtg
     7861 cacgaagccg ccgacggcga gcaggcgttg cggttggtcg acgaccttct tccccgcgcg
     7921 gcgatcatcg acatctcgat ccccggcatg gacggttggg agacgacgcg acgcctcaag
     7981 ctcgacgagc gcacgcgcga cgtgccggtc ctgctggtga ccggccatgc gctcgacgag
     8041 gaccgccagc gggcgcaaga tctcggctgc gctgcctatc tggtcaaacc cgttctgccc
     8101 tcgcggttcg tcgaggaagt ggcgcgcttg gtggagaaac cctgaagaac gccacgccga
     8161 gcatggatca gcccaatgga cgcacagcgg ccgccgctcc cacggacacc ggcggttgcc
     8221 tccgcgtgct cgtcgtcgat gcggacggga aggtggcagc ccaactcgcc acggcgctcg
     8281 aaggctcgcc agagcgcgaa cggttcgagc tagcgcgcgc ctcgggcctg acggagaccc
     8341 tcgaaaagct tgctgccggg gacaccgacg tcgtgctgct gccgatctcg ccgctcgacc
     8401 agggactcgg gcctctggtc accatccgcg cggaagctcc caacctgccc gttgtcgtgc
     8461 tctgcgcgac cggggacgag cctctggccg tcaaagccgt gcaactcggc gccagtgact
     8521 acctggttgc tgagcggctc tacggcaccc tcgctgcgcg ttcgttgcgc cacgcggcgg
     8581 aggtggcacg aatgcgggcg gtgcttgccg cgcatcatgc ggagtggcca gcctcgctgg
     8641 gcgaaagagc gagcagccgc ccggcgccgc tacgggcagc gttccctgag cgcttcgccg
     8701 agctcgttcg tgattacggg gagatcctcg atcgcgccgt ggagcagatc cttcagcggc
     8761 agctggcgcc attcgagcac cggctgcaga agctggccag cgtagcggcc gacctgcgcg
     8821 ccgggccgcg cgatatcgtc gagttgcatg ccacggccat gaagcagcgg gaggccgagc
     8881 aggggccaca gcgcatgaag ctctacctcg cggaaggccg cctgcgattg ctcgagctca
     8941 tggggtatct ggtcgctgtc tatcgcaacc gctggctggg aggtccgagg tccggggcgg
     9001 attgaggaca cgcacacgaa aggatggatg acctggaccg atacgtgctg aagctctaca
     9061 tcagcggtcg atcgccgcga gccgagcgcg cgatcgctaa cctccggcgc ctctgtaagg
     9121 acgagctcga ggggtgctat gagctcgaga tcatcgacgt cctcgagcac ccgcaactgg
     9181 ccgaggatga ccggatactg gccacgccga cattgatcaa gcaactgccg ccgccgctac
     9241 gacgagtgat cggggatctg tccaatcacg acaaggtgtt gctggggctc gatgtgcggc
     9301 cgcgtgacga acgtccgcgg gagggagaga agccgtgacg aggagaagcc aatgagcgag
     9361 cgggtgcttc caccggctaa agtgttgacc ggcattcccg gcttcgacaa tctcacctat
     9421 ggcggcctac ccgccaatcg ttcgacactg gtctccggca gctcgggaag cggcaagacc
     9481 atcttcgccg tgcagtacct cgcccgcggc atcaccgagc ttggcgagcc cggagtcttc
     9541 gtaaccttcg aggagagtcc cgacgacatc cgccggaacg tcgcaagctt cggatgggat
     9601 ctcgccgctt gggaagagga cgggaagtgg gccttcgtgg atgcctcacc cgatcccgag
     9661 gaggagatcc tcgaggccgg cgactatgac ctgggcggcg tgatggcgcg gatcaaacat
     9721 gcggtcgccc gcaccggcgc gcggcgtctg gtggtcgact cgctctctgc cgtcttttcg
     9781 cagttccagg acgcgtccgt cgtgcgcaac gagttgttcc gcatcgcccg cgccgcccgg
     9841 aagatgggcg tcacggcggt gctaaccgcc gagcggactg aagagtacgg cccggtcggc
     9901 cgttacggag tcgaggagtt catcgccgac aatgtagtgg tgttccgcaa tgtgctggaa
     9961 gaggagcaga ggcggcgcac gctggagctc ctcaagttcc gtggaacgga tcaccagaag
    10021 ggggagcacc cgttcgtgat cgaggcagac cgcggcctgg tggtgactcc gctcaccggt
    10081 gttcccctcg agcagcgatc gtcgaacgtc cgcgtcacct ccggcaatcc cgagctcgat
    10141 gccatgtgcg gcgatggctt ctttcgggat tccgtggtcc tggtctccgg ggccaccggg
    10201 accggcaaga cgttgacggt cactcacttc gtcgctggcg gagcgcgtga gggcgaccgc
    10261 ggattgctgt tcggctacga ggaaagccgc gagcaactgg tccgcaacgc cgccggatgg
    10321 ggagtcgatt tcgggagcat ggaagatgcc ggcatgctgc gggtccactg cgagtatccg
    10381 gaggcgcgtg ggctgcagga ccacctgctg cggatcaagc ggatgatcga ggagttcgcg
    10441 ccgcagcgcg tggtcatcga ctcgctctcg gcgctcgagc gggtagggcc gccaaaggcg
    10501 ttccggcaga tggtgatcga tctcacctcg ttcatcaagc agaagtcgat caccggcctg
    10561 ttcaccaaca ccacgcccac actggttggt ggtacgtcgg tgaccgaagc tcacatctcg
    10621 agcctgactg attcgatcat cctgctgcgg tatgtcgaga tgttcggtga gatgcagcgc
    10681 ggcctgatgg tgctcaagat gcgaggctcg ccgcacgaca aggaaatccg ccaagtgaca
    10741 atcgatgcca ccggtatgca tctcggcgaa cccttccgct ccgtgagcgg cattctgagc
    10801 ggcaatcccg tgcactcgcg cgaggaactg gctcggctgg aggggctctt cccccaggaa
    10861 tgagcctggc acagcgcctg catggaggcc cgtgcaacgg cgggtgacag tctccagacg
    10921 agagcgaact ccatgagcgg caagggtcta aaggtaaaga aagtcgcgat ttcaccaccc
    10981 gatttccggg tcttgttcga gtccgcgcct ggtctctatc tggtcttgac gccggatctg
    11041 cgcatcatcg ccgccagtga cgcgtacctg caggcgacga tgacgaagcg cgaggagatc
    11101 ctcggccgtg atctcttcga agtgtttccc gataaccccg aagacaccgc agccagcggc
    11161 acacgcaacc tgcgcgcctc tctcgagcgg gttctcgcga cgaagaagcc ggatgcgatg
    11221 gccgtgcaga aatacgacat ccgccggccc gaggaggata gcggcgggtt cgaggagcgc
    11281 tactggagcc cggtgaattc gccggtcttc gcaaccaact ccgagatcgc ctacatcatc
    11341 caccgcgtcg aggacgtcac cgacttcgtg cgtctgagac aacaaggtat cgagcagacg
    11401 cgccgtacgg aggccctgca gagcgaggcg gagaggatgg aagccgagat cttcctgcgc
    11461 gcccagcaac tgcaggaagc caacgcgcaa ctccgcgagg tcaatgcgca gatcgaacag
    11521 cgcgataccg agcgcgagcg gatcatcgag gggaccggcg atgcgatggt cgtcgtcgat
    11581 ctggagggct cggtgcggtt tgccaactcc gccgccgagg cattgttcgg ccaacggcgc
    11641 gagaagctcc tgggcagccc cttcgggttt ccggtggtgt ccggtgagac caccgagctc
    11701 gatgtggccg gcagccgggt ggccgagatg cgcgtcgtcg gcctgctgtg ggaggggcgt
    11761 agcgcctatc tggcttcgct gcgcgacatc accggccgca aggaggccga ggacgcggag
    11821 cgccgcctgt ggcgggagcg caccgcgcga gaggaaagcg agaaagagcg gcggcgtctg
    11881 caggagctcc tggagcgtgc ccccgcggcg gtgttgacca ctcgcgggca ggatcacgtc
    11941 tgcgtctttg ccaacccccg cgcgaccgag ctggtggggg ggcgcgagct gctcggccgg
    12001 ccgctggccg agacccttcg agagctccgc ggccagggct tccttgaagc attcgacagg
    12061 gcgttccagg atgtggcgag cggtgccagt cccgagctcg agctgtcgct cgccggcgag
    12121 cggggggagg caggggaggc ctggcgttgc gtcgacgtca cctgggtacc cctcggccag
    12181 gatgggcggg tcgacggggt catgtgcttc gcgcacgatg tcaccgagca ggtggcgatg
    12241 cgacgggcgt tggagcagac catggagcgg ttgcgcgagg aggagcgccg caaggaccag
    12301 ttcatggctg tgctcggcca tgagctccgc aacccgctgg cgggcatcga tggcggactg
    12361 cgcctgcttg cagggggagc cgaccccgat cgggagcagt gggcgttggc catgatgcgt
    12421 aaccaggtgc gccagctcac cagcctgctc gacgaactgc tcgatgtctc gagcatcgcg
    12481 cgcggcaagc tcgagctgcg acggagggtg gtcccgctga ctcagattgt ggaggccgcc
    12541 accgcgtcga ccgcagcgcg gctgtccgac tttgagcagc gtctgatcgt ctctatgccc
    12601 gacgaacccc tgcacatgga cgccgacccg cggcgcatcg aacaggtgct cgccaacctg
    12661 ctggtcaacg cgtccaagta ttccgatcgg ggaacggaga tccgcctcga ggtacgtagc
    12721 gaggcggagg aggccatcat cgaggtgagc gatcagggct tcggcatcga tcctcagatg
    12781 atcgagcgga tcttcgagcc cttcatccag gcgccacgcg gagacggtgg gaagatggcg
    12841 ggcggcctcg gcatcgggct gaccctggtc aggcagctcg ccgaactgca cgggggctcg
    12901 gcggtggccc acagcgacgg gctaggcagg ggcagcacct ttaccatccg gctgccgatc
    12961 gctgcggagg cggctccgga ggtgccggcc gagagcaccg cgaccgtctc gttggcagag
    13021 caccggatcc tggtggtcga cgacaacgtc aacgcggcga cagcgctcac cgagttgctg
    13081 aagcttcgcg ggtgcaacgc gcgggcagca atcagcggcg gcgaggcgct gacggccgcg
    13141 agcgaggagg ttttcgacgt catcctcctt gatcttgacc ttccggacat cgtggggtac
    13201 gaggtcgcca ccgagctccg gcgccggccc gccggctgcc gcctgctcat tattgccgtg
    13261 tccggcttcg gcgacgagca ggcccgcgag cgctcgcgca agagcggcat cgaccaccac
    13321 atggtcaagc cggtcgagat cgacgagttg ctgcgggtca ttgccagcgg cacccttgtc
    13381 accagataag caacctaagc aaaacaagag caaagccgcc tacctcgtac tcgagtgggg
    13441 aagggatggg ggccatccgt ttggcccgcg cgaggagggg ccgtgaggcc cgtagaggtg
    13501 gccggcaagg ctgacggcaa ggctgacgag tcacccactt gtagaggctt ggcttggaag
    13561 aagcttggaa gaagagtcac ccatttgtag agtagagaac tggaagaaga gtcacccatt
    13621 tgtagagtag agagctgtaa attgcccgtt ccatagtgct gatcgggcat aggatggcgg
    13681 ctgaagtggg tagaagccgg ctgaagcccg tccgatgaag tgtctcgtgg ttctgagtgc
    13741 ctgtttcgga ggcgcttctg aggcaggaat gccccgagca gatagacctc tcccttgttc
    13801 agtggcgtcg gcgcccaccc atcgatcagt tcgcgactgg ccgaagttgc ttcgtcagcc
    13861 ggcagtcgcc cttcgcacga acggcagggc cggcgggaag ctcccttccg ggaactccac
    13921 gagccggtcg cccgccctca acttctcgga cgcatccctg aacgccgcta cgaagcggct
    13981 ataagcctcg cgcagcgcca ggtatgcccg cttgctcgcc gtgtggaaca gcggcgcggg
    14041 cgacttcttg gtccggtttg gacgggtatg cggttgctgc ctgcgcacca tgtccgctcc
    14101 cagaggctcg gtgccctttg cctctctcac tacccgggct tcctccacga tctcagccac
    14161 caggtcgacc atgcgccgcc ggtactcttc cggcggcagg tgcctccagc aggggagggg
    14221 agcgagggtc aggacctcct cggtcgcgaa ctggcgcggc tcgggctcct tgccgcgcaa
    14281 ccggtaccgg tactcctggg tccggtcgta ccagactccg accacgggct ctccgctcag
    14341 cagggcatgg accgcgtggg cgcccggcca ttcgcgagga tcggcgacca ggccctcctt
    14401 ggccccatga gaaaggatgt acttcaggcg cgcgatctgg gccccctctt cgtcgctgac
    14461 cggaatggac tgatagcggc ggccccagaa cctctctttc cagtccgcca gacggccgag
    14521 ctcccgggcg aggttaccgt tcaggtagcc catgaaggcg gacatctgac cggcatcctc
    14581 cacccagatc aagagatgga agtgatttga ggcaaaggcg aagacgatca gatccagcgg
    14641 atacatccgt ttggcccgcg ccaggacccc gaggacgatc tcctggacat gggcacgagg
    14701 cgtgagcagc aggcggcttt ggacggtgcg gcaggtcacc tcgacgagtg acccgccttc
    14761 tgggacatgg cggagttttt tcggcatgcc cctaaaagac ggaaaacgcc ggaaaaatcg
    14821 ctcaatttta gtttatcggc aaccgtctat tacggggaga ccccggaggg agccgtgcct
    14881 ggacccgatc ttgctgcccg tcggccggag ggaggacgtc atgagccaga ccaatgaacg
    14941 gacgagccaa gctcctcacg ggggaccggc cgatagcgag catgaccaga aggtcccaga
    15001 gatcgacctg ctcagcccct tgactctgcg gggtgtcacc ctgcgcaacc gcatcgtcat
    15061 gtcgcccatg tgtcaataca gcgccgagga aggtctggcc aatgactggc acctggtcca
    15121 tctcggcagc cgggcggtgg gcggggttgc cctgatcatc gtcgaagcca cggccgtcac
    15181 ccgcgaggga aggatctcgc ctcgagacct gggaatctgg ggcgacgagc acgtcgagcc
    15241 gctggcgcgc atcgcccgct ttgtccactc ccagggagcg gtccccggca tccaactcgc
    15301 ccatgccggg cgcaaggcga gctgtgagcc gccctggctg ggcggcgcac ccatcccggc
    15361 ggaccggccg gacggctgga ccgtcgtcgc tcccagcgcg gtgccgttcc acgaggcggg
    15421 tgcggtcccc gtgccgctcg acgaggccgg catcgacggc gtcgtcgccg cgttcgaggc
    15481 cgccgcccgg cgcgcgctgg aggcgggctt ccgtgtcatc gagatccacg ccgcccatgg
    15541 ctacctgctg cacgagttcc tttcaccgct cagcaaccaa agagacgacc actacggcgg
    15601 cagcctggag aatcgcatgc gcctcctgct gcgggtggcc gggcggctgc gggcgctgat
    15661 gccggaggaa ctgccgctct tcgtgcggat ctcggcgacc gactgggtcg aaggaggctg
    15721 ggatgtcgag cagtccatcg agctggcgaa gcggctgcgc gaggcgggcg tggatctgat
    15781 cgacgtctcg tcgggcggga ccacgccgcg ggcgcgcata ccggttggca agggttatca
    15841 ggtgcccgcc gccaggcgca tccgcgatga ggcggacgtc aggaccggtg cggtggggct
    15901 tatcaccgag gcgtcctatg ccgacgaaat catcacgggc ggggacgctg atctggttct
    15961 cctcggtcgg gagttgctgc gggagcccta ctgggcgctc aaggctcagc acgagatgga
    16021 cgaggagccg gcctggccca tccagtacgg gtacgccgtc aagcggcgct cccggtgagg
    16081 ccctcggaac gcgaggtttt cccgcggcgc cttccaaaca gaaacagtgc atcccgaccg
    16141 gatgctgttg gagcttcgag gacctgggcg ccgatcctcc aagcaggggc tggacagtgc
    16201 caccgaaaag agtaggattc tttaaatgat tgagccggcg ggaacgccgg ctcctcaacg
    16261 agggaggtcg catgcgcaag aagttgctca ccctggccct ggtgttgact gcagccgttg
    16321 ttgggacgct ggctcctcaa gccagcgagg ctctgccttg ctggacctgt gaccctgcga
    16381 cgaggtgctg ctgggatttc tgcgagagtt acaggtggtg ctactgacga caggtagccg
    16441 gaaatcagtt ccacgacagg cgcccctcac tgtaggggcg ccccgcgggc gttcaaaggg
    16501 tattgcccag gcctgggcgg cgggggtgct ggtgggggag gctcggtgag ggccgccaag
    16561 aatccgcttc ttcggctaca atcgcgaaat cttgacggat cgacctgctg gagatcatct
    16621 ccagggcgaa acgatcggcc cttatcgcct ggatgcgccc atcggctccg gcggcatggg
    16681 gacggtctgg cgtgcctggg acgagcgctt gcggcggcat gtggccgtca agcgggtgct
    16741 tcgggattcg gaggtccgcc gcgagcggtt gcgtcgcgag gcgcaggccg ttgcccggct
    16801 cagtcatccc gcgatcgtcc acgtctacga cctgctcgaa ggtgacggcg cggactggat
    16861 cgtcatggag ctggtcagcg gcgaggacct ccgcaagatt ctcgacagga ccgcgcggct
    16921 cgacgtaggg caagcccttc atctcggccg gcagatcgcc gaagggctcg ccgaagcgca
    16981 tgcccagggc atcctgcatc gtgatctcaa ggcggccaac gtcatggtca ccgcgtccgg
    17041 gcgcgccaag atcctggatt tcggcctcgc caagacgctg cgcggcgaag gttccggcgc
    17101 ggccgatgtc gaccagtcgc tgtcgctccc cggaaccgtg ctcggcacct gctacgccat
    17161 gtcgcctgag caggctctcg ggcacgatct cgacccccgg tccgatctgt tttccctcgg
    17221 ctctctgctt tacgagatgc ttaccggcag cgcgccgttt cgcgccgagg atgcccggtc
    17281 ttcgctggcg cgggtcctct atttccagcc ccgtccgctg cgcgaggtcc ggccggaggt
    17341 gccttccggt ctctcctcgc tgatcgagcg cctgctcgag aaggagcctg ccaagcggcc
    17401 tgccggcgcc ggtgaggtcg ccaggatcct ggcgaccctg gcaacaacct ctcttgaaag
    17461 ctccaactcg tccggtgccg gtggcaaggt ggcagcgagc accgcgaatt ccatcctgga
    17521 gacgatcgtg gagaggttgc ctgtcgccga ggtgccggca cccataaagc cgcctgccgg
    17581 cgaaggccgc cgcgatccca gcagcggggt cggcggcgag cggcggaccg tcacagtggt
    17641 ctgttgcagc gtggtgagcg ccgaagccgc ggtccccctc gagctcgagc tcctctcggc
    17701 gtcaatgagc agtatcgaga gcctggccca ggaggtctgc cgggaccttg ccggccacct
    17761 gggagccgtc ctgagtcaca tggtgtggat ctacttcggc tatcccgtca gccatgagga
    17821 cgatgcggtg cgggctgtgc gggcggcccg cgagctgatg gtccgcgccc gggaggcggg
    17881 actgctgcgc cggggactcg ccctgcgctt cggaattcac accggacccg cggtcgtgtg
    17941 gctccgtccg ggccaagcgg accgcctgca actggggagc accttcgact tcg
//
LOCUS       DASPJI010000297         5273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_762527, whole genome shotgun sequence.
ACCESSION   DASPJI010000297 DASPJI010000000
VERSION     DASPJI010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762527"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VEL74_07935"
     CDS             <1..556
                     /locus_tag="VEL74_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62497.1"
                     /translation="SEEAVDGGWLEYCHARGIFDCLGEAEVMGEEVTFKMNGDDALAL
                     VDHFSAPSKSRNVTWSNFQMRGLTTSGAEISATLDQTRKSSGTLLSVGNTKFPATATM
                     RFFLRLETGGMTLVSTKPAVFQGIVNSIAPSPGDSLALISGPVEFYEEGGNPDVKMAT
                     LNKSRVVYRRALPLEVEPAGNVKQ"
     gene            1366..4035
                     /locus_tag="VEL74_07940"
     CDS             1366..4035
                     /locus_tag="VEL74_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62498.1"
                     /translation="MTPTLASGGAGRRDLWVSLLIGLCCLLVYNANRRAISSGDAYPA
                     RYLPFAIWQHHTVLLDPIAPVTNQGRGGTAHWMVPQADGRIVSLYPVTLPVLISPLYL
                     PAVAYLRLEGWTERRLDHVARIMEKLAASFVAALSAALLYLLLRRRVTVPTALLLTIA
                     YAFGTTTWVISSQALWQHGMAELLLVGALLCLTAPCTTPRALAAGLLCGLVAANRPPD
                     LILAAALGAYGLFWAGRRAPRLAALIGAAALPMVLALAYNLAVADHFAGGYGRVGKAT
                     FFEHDLLSGLAGLLFSPTRGLFVFSPFLLFLVLAWRYRPRGPLDRDERGLTLAISIGV
                     VLQLLLYAKTDWRTGISWGPRYLTDLLPLLVWLLAPVVAALRGFGRALFVLAVGAAVV
                     IQAIGAFWYTGVTDAALYAVTSGPHRMRAAWDWRNAPFVASLQHGLAPAELMFEVRGS
                     FDAIETAGGPAFAVSAGQEAYAAGWALAGDAAPWQVGVLIDGRLTAVSRSFHDRPDVR
                     AALNEASPAGWRVPVDTAGLAPGKHRVAVLAWAFENGAGHYLGEHELTVRTAGEELED
                     GFKTAAARLRARQQGPGYWLTTHTITTRFQQPLQEMNTYLTALLIDLLEPLASTGAPG
                     DLGDSLRRARRHLTEQIEAGGLVRYHGLPDAPGIGTLGCAITPDSDDTALAWRIAPSP
                     DRRRLPAALATLDRYRTREGLYRTWLAPVEDYRCLNPGSDPNPADLTIQMHVLLMLAE
                     ERPAAGRALCEALRPVVDQDRVWVYYRRAPLVPILRLPDLRRAGCDLELPESRMRTEV
                     EGQEIWVSAARLLARAPASADRSADAAEARAVLRRLAQDDFALLRESPPLLYHNDLTA
                     SVSRYYWSEDVGYALWLRLYHEYSHPGHPPLAG"
     gene            3995..4903
                     /locus_tag="VEL74_07945"
     CDS             3995..4903
                     /locus_tag="VEL74_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HYG62499.1"
                     /translation="MSIHTPVIPLSPVRAAAARYLSCLRLPEILVLQGSPLLGAAFAL
                     RQPAAEHVGPLALLIAANVFLVAHIFMLNDWAGLTTDLADPNKAAGVFTARGVGRKEI
                     GGLTAGLLALSLLLFSRLGPGTLGLALGIAAVSALYSLPRFNWKGRPLLNSAAHLIGG
                     TLHFLLGYSLGNAIDGRGLATAAFFALIFAAGHLTQEIRDHHGDLLNGIRTNAVAFGR
                     RRTFAASLVLFTLAQALLLVLALQGTLPRPLAALVALYPLHLRWSLQTLAEGLTFASI
                     CRLQARYRVLYAIIGLAMVAGLCGGF"
     gene            complement(5081..>5273)
                     /locus_tag="VEL74_07950"
     CDS             complement(5081..>5273)
                     /locus_tag="VEL74_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62500.1"
                     /translation="NDRRLNRTELRDRGGWEGRFEDMDDNRDGLLTTREWNGRRGDFD
                     RLDRNNDGRISLYEWLRQT"
BASE COUNT          709 a         1942 c         1748 g          874 t
ORIGIN      
        1 gtcggaggaa gcggtagacg gcggctggct cgagtactgc catgcgaggg gcatcttcga
       61 ctgcctgggg gaggccgagg tgatgggcga ggaagtcacg ttcaagatga acggcgacga
      121 cgcgttggcc ctggtcgacc acttctcggc cccctcgaaa tccagaaacg tcacctggtc
      181 caacttccag atgcggggct tgaccacgag cggcgccgag atcagcgcca cgctggacca
      241 gacccgcaag tcgtccggca cgttgctctc cgtggggaac acgaagtttc ccgccaccgc
      301 gaccatgcgg ttcttcttgc ggctcgagac cggcggaatg accctggtct ccaccaagcc
      361 ggcggtgttc cagggcatcg tcaattccat cgcgccctcg cccggtgatt cgctcgccct
      421 gatcagcggc ccggtcgagt tctacgagga gggtgggaat cccgacgtga agatggccac
      481 gctgaacaag tcgagggtcg tctaccggag ggctctccct ctggaggtcg agcctgccgg
      541 caacgtgaag cagtaagccg cggcaatctc tctcttcgcc gtgatcggcg ctatggcgct
      601 tcctggcgcc cgtagccgtc ggtcgcggcg aacggctctc tgagcaaccc gacgccaccg
      661 tccgcgccgg ccatccccgc ccaaacaacg atcatccccg agccaagaag ggccttaccc
      721 agggtaggag cgccgtaccc atgggatggc cgccatttcc cgaggcaggg cctccgtacc
      781 cgtggacagg gcctctctac ctactcgcgg cgtgccgtac ccacgggtac ggggccgtcc
      841 ccacgggtag ggagtccgcc ccgggaggga tgacggccat ctcccggccg gcaacggttg
      901 cggagcccat tccaccgggt cccggaggcg gaggacgtcc cctccgcctc cctacgacgc
      961 ggcgccgcgg ttcaggagcg cggccttgga tccggcggcg ctcccgcaag attcagttgt
     1021 caaagaacgg ggagagctgg ctccgagccg agcccgaatc tgcccccaaa gaagtgtaca
     1081 gagaatacac ctcgaacaca aagactaagt tgacattctg gacgggattg gcgacaatga
     1141 atgctgcaag cgggggacca aggtcccacc aggaaggttc tttgacaatt gatgggcatg
     1201 gttctgggca cggcgggggc ggcggccgtg cccgggaccg tagtgtgagc gaccgcttca
     1261 gcttggcgcc ggcggcgccg tgtgctagcc tccggcctcg cctcccgaca accttgtagt
     1321 ggagtcaggg ccccgtagga cgtctgtgcg agtgagcagg aaaccatgac tccgaccctc
     1381 gccagcgggg gggccggccg gcgtgacctg tgggtgagcc tgctcatcgg gctctgctgc
     1441 ctgctggtct acaacgccaa ccggcgggcg atcagctccg gcgacgccta cccggcgcgg
     1501 tacctgccgt tcgcgatctg gcagcaccac accgtcctcc tcgatccgat cgcgccggtc
     1561 accaaccagg ggcgcggcgg gacggcgcac tggatggtgc cccaggccga cggccgcatc
     1621 gtctcgctgt atcccgtgac gctgccggtg ctcatctcgc cgctctacct tcccgcagtc
     1681 gcttacctgc gcctggaggg ttggacggag aggcgactgg accacgtggc aaggatcatg
     1741 gagaagctcg ccgcctcgtt cgtggcggcg ctctccgcgg cgctcctcta cctgctgctg
     1801 cggcgccggg tgacggtgcc gaccgcgctg ctgctcacca tcgcctatgc cttcggcacc
     1861 accacctggg tgatcagcag ccaggccctg tggcagcacg ggatggccga gctgctgctg
     1921 gtcggcgccc tgctgtgcct caccgcgccg tgcaccacgc cgcgggcgct ggcggcggga
     1981 ttgctgtgcg ggctcgtcgc cgccaaccgc cctccggacc tcatcctcgc ggcggcgctc
     2041 ggcgcctatg gactgttctg ggccggccgc cgggcgccgc ggctcgccgc gctcatcggg
     2101 gcggcggcgc tgccgatggt cctggcgctg gcctacaacc tcgccgtggc cgaccacttc
     2161 gccggtggct atgggcgggt cggcaaggcc accttcttcg agcatgacct gctctccggc
     2221 ctcgccggcc tgctgttcag cccgacgcgg gggctgttcg tcttttcccc gttcctgctc
     2281 ttcctcgtgc tggcctggcg atatcgaccg cgggggccgc tggaccggga cgagcggggc
     2341 ctcaccctgg cgatcagcat cggcgtcgtc ctccagctcc tgctctacgc caagaccgac
     2401 tggcggacgg gcatctcctg ggggccgcgc tatctcaccg acctcctgcc gctgctcgtc
     2461 tggctgctgg cgccggtggt tgccgcgctg cgtggcttcg gccgggccct gttcgtgctc
     2521 gcggtcggcg ccgcggtggt catccaggcc atcggcgcct tctggtacac cggcgtgacc
     2581 gacgccgcgc tgtacgccgt caccagcggt ccccaccgga tgcgggccgc ctgggactgg
     2641 cggaacgcgc ccttcgtcgc gtccctccag cacggcctcg cgccggcgga gctgatgttc
     2701 gaggtgcgcg gcagcttcga cgccatcgag accgccggcg gcccggcctt cgcggtcagc
     2761 gccggccagg aggcctacgc cgccggctgg gcgctggcgg gcgacgcggc gccctggcag
     2821 gtgggggtcc tcatcgacgg ccggctgacc gccgtctccc gctccttcca cgaccggccc
     2881 gacgtccgcg cggcgctgaa cgaggcgagc cccgccggct ggcgcgtccc ggtcgacacg
     2941 gccggcctcg ctcccggcaa gcaccgggtg gccgtcctcg cctgggcctt cgagaatgga
     3001 gcgggccact acctcgggga gcacgagctg accgtgcgca cggccggcga ggagctcgag
     3061 gatggattca agacggcggc ggcgcggctg cgcgcgcgcc agcaggggcc gggctactgg
     3121 ctcaccacgc acacgatcac gacgcgcttc cagcagccgc tccaggagat gaacacctat
     3181 ctcaccgcgc tcctgatcga cctgctcgag ccgctggcgt ccaccggtgc tcccggcgat
     3241 ctcggcgaca gcctgcggcg cgcccgccgg cacctcaccg agcagatcga ggccggcgga
     3301 ctggtgcgct atcacggcct gcccgacgct cccggcatcg gcaccctggg gtgcgcgatc
     3361 acacccgatt ccgacgacac ggcgctcgcc tggaggatcg ctcccagccc cgaccggcgg
     3421 cggctgccgg ccgccctggc gaccctcgac cgctaccgca cccgcgaggg cctctaccgt
     3481 acctggctcg cccccgtcga ggactaccgg tgcctcaacc cgggcagtga tccgaacccg
     3541 gccgacctca ccatccagat gcacgtgctg ctgatgctgg ctgaggagcg gccggcggcc
     3601 ggccgggcgc tctgcgaagc gctccgtccg gtcgtcgatc aggaccgggt ctgggtctac
     3661 tatcgacggg cgccgctggt gccgatcctg cgcctgcccg acctccggcg cgccggctgc
     3721 gacctggagc tgcccgagtc acggatgcgg acggaggtgg agggccagga gatctgggtc
     3781 tccgcggccc ggctgctggc gcgcgcgcca gcctcggcgg accggtcagc ggacgccgcg
     3841 gaggcccggg cggtgctgcg ccggctcgcg caggacgact tcgccctcct gcgagagagt
     3901 ccgccgctcc tctatcacaa cgatctcacc gcgtcggtct cccgatacta ctggtcggag
     3961 gacgtcggct acgccctctg gctgaggctc tatcatgagt attcacaccc cggtcatccc
     4021 cctctcgccg gttagagctg ccgcggcgcg ctacctgtcc tgcctgcgcc tgccggagat
     4081 cctcgtcctg cagggctcgc ccctgctcgg cgccgccttc gcgctccgcc aaccggcggc
     4141 ggagcacgtg gggccgctgg cgctgctgat cgccgccaac gtcttcctgg tggcgcacat
     4201 cttcatgctc aacgactggg ccggcctgac caccgacctg gccgacccca acaaggcggc
     4261 cggcgtcttc acggcgaggg gtgtgggccg caaggagatc ggcggcctca cggccggcct
     4321 gctggccttg agcctgctcc tgttcagccg cctcggcccg ggcactctcg gcctggccct
     4381 gggcatcgcc gccgtgagcg ccctctattc cctgccccgc ttcaattgga agggcaggcc
     4441 gctgctcaac tccgcggccc atctgatcgg cggcaccctt cacttcctcc tcggttattc
     4501 actggggaac gcgatcgacg gccgcggtct cgccaccgcc gccttcttcg ccctgatctt
     4561 cgccgccggc cacctgacgc aagagatccg cgatcaccac ggcgacctcc tcaatggcat
     4621 ccggaccaac gcggtggcct tcggccggcg gcgcaccttc gccgccagcc tggtcctctt
     4681 caccctggcc caggccctcc tgctcgttct ggccctccag gggaccctcc cccggcccct
     4741 ggcagcgctc gtcgcgctct acccccttca cctgcgctgg tcgctccaga cgctggccga
     4801 ggggctcacc ttcgcgagca tctgccgcct gcaagcccgc tatcgcgtgc tgtacgcgat
     4861 catcggtctg gccatggtgg ccggcctgtg cggcgggttt tagggtgtag actacaggga
     4921 ctcgggcatc ggaccagtgc ttgtgttcga gtaggcctgg cggagttcgt gagacgactc
     4981 cgttgggccg ctaccgatct ccggcgctct ttatcggcgc accggccagg atggtgggca
     5041 ctcgtggtga cccgtaatag ttccccctcg ttgagcagcg ctatgtctgc cgcagccact
     5101 cgtacaggct gatcctgccg tcgttgttgc gatccagacg atcgaagtct cctcggcggc
     5161 cgttccattc cctggtggtc aagaggccgt cccggttgtc atccatgtct tcgaaacggc
     5221 cctcccaacc gccacggtcc cggagctcgg tacggttcag ccggcggtcg tta
//
LOCUS       DASPJI010000298         3158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_77419, whole genome shotgun sequence.
ACCESSION   DASPJI010000298 DASPJI010000000
VERSION     DASPJI010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77419"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3158)
                     /locus_tag="VEL74_07955"
     CDS             complement(<1..>3158)
                     /locus_tag="VEL74_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG62501.1"
                     /translation="DLARFRSDGDVEYLGRNDHQVKLRGFRIEPGEVEAALTAHPAVR
                     QAVVEARDVQGERRLVAWVVVADAGGGEVPADLLEELRQRLPEAMVPSLIVPLAALPV
                     TASGKVDRRALPAPDRALPRTAGGAAPRTPVEEALAAIWREVLGLDQVSVDDSFFDLG
                     GHSLLATRVASRVRHAFGIELPLRRLFEAPTLARLAAVVEESLEPGEVAALARSGDGA
                     EAPTSFSQQRFWFLHRLDPRSSAYNMSLALRLGEGLDGRALERALEEIVRRHEALRTT
                     FQERDGEPVQVVAPPGPLTLPFLDLSALGGEVDGEEARSLVEEICRQPFDLAGGPLLR
                     TALLRLPAGDHVFAVCMHHIVSDGWSLEVFADEMRVLYGAFAAGRPSPLPELPLQYRD
                     YAVWQRRRLAGEELAADLAFWREELRGLPAVLDLPADRPRPALQSFHGGLVSGDLPPG
                     LAAELRSLARRQDATLFMVLLAAFQALLGRLAGVDDLAVGVPVSGRDRLEVEGLIGLL
                     INSVVMRGDLSGDPGFLELLRRARERSLAVQAHQAFPFEKLVEEVQPERSLSHAPLFQ
                     VMFAFGASRERRSGGEAALQALPLRQRTAQFDLTLVVEDENGLRAGWEHSSALFDEPT
                     VRRYAGWFSTLLAGAVAEPERRLSELPLLTAGEEAQILGAWNDTGARLLPERGTLVGR
                     FAERVRRTPDAPAVISGDEELSFRELGAWARRVARRLRLLGVGTETVVAVCLERSAAA
                     VAAALGAMEAGGVWMPLDPVYPAARHALLLADSGAGVLVTRRELLSSLPAGALPPVLL
                     VDDSLEDTPEDEAWEAPRVDSSHLAYVIYTSGSTGEPKGVGVAHGPAADHCATIAREY
                     GLGPGERVLWFASPGFDATVEQVLPTLLAGAALVPRGQELWEPASLSAALEGLGITAV
                     DLPPAYWQQWVEAGGEACATLRTVNVAGEAMPVPAARQWRDTPIGRCELINGYGPTEA
                     VVTATLFRVGADFEPRAGAAAVPLGRPLAGRVALVLDRSGSLRPAGVPGELCLGGGLL
                     ARGYLGRPALTAERFVPDPWSP"
BASE COUNT          453 a         1247 c         1144 g          314 t
ORIGIN      
        1 cgggctccag gggtccggca cgaacctctc ggcggtgagg gcgggccggc cgagataccc
       61 ccgggccagc agcccgccgc cgagacagag ctcgcccggg acgcccgccg gccgcaggct
      121 gccggaccgg tcgaggacca gcgcgacgcg cccggccagc ggccggccga gaggcaccgc
      181 ggcggcgccg gcgcgcggct cgaagtccgc tcccacccgg aacagggtgg cggtgaccac
      241 ggcctcggtc ggcccatacc cgttgatcag ctcacagcgc ccgatggggg tgtcccgcca
      301 ctgccgggcg gccggcaccg gcatcgcctc cccagccacg ttgaccgtcc gcagcgtggc
      361 gcaggcctcg ccgccggcct cgacccactg ctgccaatag gcgggcggca ggtccacggc
      421 cgtgatcccc agcccctcca gcgccgcgga caggctggcc ggctcccaca gctcctggcc
      481 gcggggcacc agcgcggcgc ccgccaggag ggtcggcagc acctgctcga cggtggcgtc
      541 gaagccgggg gaggcgaacc agagcacccg ttcgccgggg cccaagccgt actcgcgggc
      601 gatggtcgcg caatggtccg ccgccggccc gtgcgccacc cccaccccct tgggctcacc
      661 cgtggagccg gaggtgtaga tgacataggc caggtgggaa gaatccaccc gcggcgcctc
      721 ccaggcctcg tcctccgggg tgtcctccag gctgtcgtcc accagcagca ccggcggcag
      781 ggcgccggcc ggcagggagg agaggagctc ccggcgggtg accaggacgc cggcgcccga
      841 atccgccagc agaagggcat ggcgcgccgc gggatagacc gggtccagcg gcatccagac
      901 gccgcccgcc tccatggctc ccagcgccgc cgccacggcc gcggccgacc gctccaggca
      961 gacggccacc accgtttcgg tgccgacccc caggagccgc aggcggcggg cgacgcggcg
     1021 cgcccacgcg ccgagctccc ggaagctcag ctcctcgtcg ccgctgatga ccgccggggc
     1081 gtccggcgtc cggcgtaccc gctccgcgaa gcgcccgacc agggtgcccc tctccggcag
     1141 gagccgcgcg ccggtgtcgt tccacgcgcc gaggatctgg gcctcctcgc ccgcggtgag
     1201 gagcggcagc tccgagagac gccgctccgg ctccgccacg gcccctgcca gcagcgtcga
     1261 gaaccagccg gcgtagcggc ggaccgtcgg ctcgtcgaac agggcggagc tgtgctccca
     1321 gccggcccgc agcccgttct cgtcctccac caccagggtg aggtcgaact gcgccgtccg
     1381 ctggcggaga gggagggcct ggagcgccgc ctcgccgcct gaccgccgct cccgcgaggc
     1441 gccgaaggcg aacatcacct ggaacagagg agcgtggctg aggctgcgct cgggctgcac
     1501 ctcctccacc agcttctcga acgggaacgc ctgatgagcc tgcaccgcca gggacctctc
     1561 ccgggcccgc cgcaggagct cgaggaagcc cggatcgccg gacaggtcgc cccgcatcac
     1621 caccgagttg atgagcaggc cgatcagccc ctcgacctcc agccggtcgc gcccggacac
     1681 gggcaccccc accgccagat cgtccacgcc ggcgagccgg ccgagcagcg cctggaacgc
     1741 cgccagcagg accatgaaca gggtggcgtc ctggcgccgc gccagcgacc ggagctccgc
     1801 cgccaggccc ggcggcagat cgccggagac cagcccgccg tggaagctct gcaaggccgg
     1861 ccgcggccgg tccgccggca ggtcgaggac cgccggcagg ccgcgcagct cctcgcgcca
     1921 gaaggcgaga tccgccgcca gctcctcccc cgccagacgg cgccgctgcc agacggcata
     1981 gtcgcggtac tggagaggca gctcgggcag tggcgagggc cggccggcgg cgaaggcgcc
     2041 gtagaggacc ctcatctcgt ccgcgaagac ctccagggac cagccgtcgc tgacgatgtg
     2101 gtgcatgcag acggcgaaca cgtgatcgcc ggcgggtagc cggagcagcg ccgtccgcag
     2161 cagcggaccg ccggccaggt cgaacggctg ccggcagatc tcctccacca gagaccgcgc
     2221 ctcctccccg tcgacctctc caccgagcgc ggagagatcg aggaagggga gcgtgagcgg
     2281 cccgggggga gccaccacct gcaccggctc gccgtcgcgt tcctggaagg tggtccgcag
     2341 cgcctcgtgg cggcgcacga tctcctccag ggcgcgctcg agggcacggc cgtccagccc
     2401 ctcgccgagc cgcagggcca gggacatgtt gtaggccgac gaccggggat cgagccggtg
     2461 gaggaaccag aaccgctgct gcgagaagga ggtgggggcc tccgcgccgt cgccggaccg
     2521 ggcgagcgcc gccacctccc ccggctccag gctctcctcg accaccgccg ccagccgcgc
     2581 cagcgtcggc gcctcgaaca ggcggcgcag gggcagctcg atgccgaagg cgtggcgcac
     2641 ccgggaggcc acccgggtcg ccagcaggga gtggccgccc agatcgaaga agctgtcgtc
     2701 caccgacacc tggtcgaggc cgaggacctc gcgccagatg gccgccaggg cctcctccac
     2761 cggcgtccgc ggcgcggccc cgccggcggt ccgaggcagg gcgcggtccg gcgccggcag
     2821 ggcgcggcgg tcgaccttgc cgctggcggt gaccggcagc gccgcgagcg gcacgatcag
     2881 ggacggcacc atggcttcgg ggagccgctg ccgcagctcc tccaggaggt ccgccgggac
     2941 ctcgccgccg ccggcgtccg cgaccaccac ccaggcgacg agccggcgct ccccctggac
     3001 gtcgcgggcc tcgaccacag cctgacggac ggcggggtgc gccgtcagcg ccgcctccac
     3061 ctcacccggc tcgatgcgga agccccgcag cttcacctgg tggtcgttgc ggccgaggta
     3121 ctccacgtcc ccgtcggagc ggaagcgggc caggtcgc
//
LOCUS       DASPJI010000299         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_312271, whole genome shotgun sequence.
ACCESSION   DASPJI010000299 DASPJI010000000
VERSION     DASPJI010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312271"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="VEL74_07960"
     CDS             <1..1336
                     /locus_tag="VEL74_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62502.1"
                     /translation="VERRYQSAAELDADLQALREGRTDVAADLDTTRRTGRPAHPPAQ
                     PPAHLTSPPPPPGAPGEALDSTRRTDRVERAVPLPPAAAATMAEMAGRAEATRRTHEA
                     PMAAGIAGAAAAPAPAAVVPPPPPVSSLFPEAELDGTLSRARPRRERKPRRWLLLGLL
                     GLLLVALGFHQMMLRSEASDIRRELVGADATALPALAERFRRVDRWAFIPSSTVQGAR
                     EELRQALVEDADRVINAFHGDTPVTTEKGWQRAYDDLRAAVDLSILDRQTRAKMVYAR
                     AHLDRIASQTLRQKGEVQAANEKLQEAVAGFRSAARWNGDWPDPYLGLARVYAYEQFD
                     LGKLESALGELGRRGYPLGRREKAMLADGYRMRGRDFQARAARARGTDDEERLLEQSR
                     ESFIEAMNQYDEIRGYANVEQNRTDAENRIDEITTRLRELDGGFSIDFEIPF"
     gene            1359..>3246
                     /locus_tag="VEL74_07965"
     CDS             1359..>3246
                     /locus_tag="VEL74_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HYG62503.1"
                     /translation="MAVTRSTPAERRQQQGQQGQQRPGVTRWVSKPPGTAAAAPRADR
                     TRETVLLAASLVVALLGMFLIYRAASHDFGQVTADLQSGKILNLEQVTEPEKLQPFMP
                     EMSGSGERAFAARKVWQRLSEKGRLPNVGELGRIRVGAAEIEDDSRLEELRERLEAAR
                     EGAAPSNAETESPTVRLFSSAELREIKPELVVRTPGEFRRTFWLWGGLLVAGFVLVHV
                     VWRLRGFQGDQVMLPILLTLTGIGFLVMVSVRDPLRDMMLFRTFAQGVLMGCGLLLAA
                     SLVDWERSPLRRMTFVPLFGALGLSLLLILFGSGPGGSDAKVNLFGFQPVEVIKILFV
                     LFLAGYFLDRWEFLRELPEKRAGLGGVPSWLRIPKLEYSLPPVLAIGAVLFFFFLQKD
                     LGPALVLSFLFLLLYSVARGRPTMMAVGAVLVVAAFMVGYQLGYPRTVTGRIGMWLSP
                     WDNAYRGGEHLAQGLWALAGGSLSGMGLGLGDPEYVPEIHTDMVLAAVGEELGFLGLL
                     AVFALYALLVWRGLRAALRSPGVYGFFLALGLTLTLALPILLISGGVLGLVPLSGVVS
                     PFLSFGRSALLANLFIAGMLLAVSARRFPGGGATEAAVVRRFEGSVRWIGLGLAAALV
                     VILGR"
BASE COUNT          393 a         1150 c         1202 g          501 t
ORIGIN      
        1 ggtggagagg cgctaccaga gcgccgccga gctggacgcc gatctgcagg ctctgcggga
       61 gggccgcacc gatgtcgcgg cggacctcga taccacccgg cgcaccggcc ggccagctca
      121 tccgccagcc cagccgccag ctcatctgac atcgccgcca cctcctcccg gggcgccggg
      181 ggaggctctg gactccaccc ggcggacgga ccgcgtcgag cgggcagtcc ctctccctcc
      241 cgccgccgcg gcgacgatgg cggagatggc cggccgggcc gaggcgaccc gccgcaccca
      301 cgaggcgccg atggccgccg ggatcgccgg ggccgccgcc gcccccgcgc ccgcggcggt
      361 ggtcccgccg ccgcccccgg tctcctctct ctttccggag gcggagctgg acgggacgct
      421 gtcgcgggcc cgtccgcggc gcgagcgcaa gccccgccgc tggctgttgc tgggcctcct
      481 cggactgctc ctggtggccc tcggcttcca ccagatgatg ctgcggagcg aggcgagcga
      541 catccgccgc gagctggtcg gggcggacgc caccgctctt cccgccctgg ccgagcggtt
      601 ccgccgggtg gaccgctggg cgttcatccc gtcgagcacg gttcaggggg cgcgcgagga
      661 gctgcggcag gccctggtcg aggacgccga ccgcgtcatc aacgccttcc atggcgacac
      721 cccggtgacc acggagaagg ggtggcaacg ggcctatgac gacctgcggg cggccgtgga
      781 cctgagcatc ctcgaccgcc agacccgggc gaagatggtc tacgcccgcg cccacctcga
      841 ccgcatcgcg tcgcagaccc tgcggcagaa gggcgaggtc caggccgcca acgagaagct
      901 ccaggaggcg gtggccggct tccggtccgc ggcgcgctgg aacggcgatt ggcccgatcc
      961 ctatctgggc ttggcgcgcg tctacgccta tgagcagttc gacctcggca agctggagag
     1021 cgccctggga gagctgggcc ggcggggcta tccgctgggc cggcgcgaga aggcgatgct
     1081 cgccgacggc taccggatgc ggggccgcga cttccaggcg cgggcggcgc gggcccgcgg
     1141 cacggacgac gaggagcggc tcctggagca gtcccgggag agcttcatcg aggcgatgaa
     1201 ccagtacgac gagatccgcg gctacgccaa cgtcgagcag aaccggaccg acgccgagaa
     1261 ccggatcgac gagatcacca cgcgcctccg ggagctcgac ggcggcttca gcatcgattt
     1321 cgaaatcccc ttctgatgag ggtcctggag gttcgcgcat ggccgtgacg cgcagcaccc
     1381 cggcggagcg ccgccaacag caggggcagc aggggcagca gcggccgggg gtcacccgct
     1441 gggtgtcgaa gccgccgggc accgccgccg cggcgccccg ggcggaccgg acgcgggaga
     1501 ccgtgcttct cgccgcctct ctggtggtgg ccctgctcgg catgttcctg atctatcgcg
     1561 ccgcatccca cgatttcggc caggtgacgg cggacctgca gagcggcaag atcctgaacc
     1621 tggagcaggt gacggagccg gagaagctcc agccgttcat gcccgagatg agcggctcgg
     1681 gcgagcgcgc cttcgccgcc cgcaaggtct ggcagcgcct ctccgagaag ggccggctgc
     1741 ccaacgtcgg cgagctgggc cgcatccgcg tcggcgccgc cgagatcgag gacgactcac
     1801 gtctggagga gctgcgggag cggctggagg cggcgcggga aggggcggcc ccctcgaatg
     1861 cggagacgga gagcccgacg gtgcgcctct tcagctccgc cgagctgcgc gagatcaagc
     1921 ccgagctcgt ggtgcgcacc cccggcgagt tccggcggac gttctggctc tgggggggcc
     1981 tgctcgtcgc cggcttcgtg ctcgtccacg tggtctggcg cctgcgcggc ttccaggggg
     2041 accaggtgat gctgccgatc ctcctgacgc tcaccggcat cggcttcctg gtgatggtga
     2101 gcgtccgcga cccgttgcgc gacatgatgc tcttccgcac cttcgcccag ggggtgctca
     2161 tgggatgcgg cctcctgctc gccgccagcc tggtggactg ggagcggtcg ccgctgcggc
     2221 ggatgacctt cgtgccgctg ttcggcgcgc tcgggctgtc gctcctcctc atcctcttcg
     2281 gcagcggccc ggggggaagc gacgccaagg tgaacctctt cggcttccag ccggtggagg
     2341 tcatcaagat cctcttcgtg ctcttcctcg ccggctactt cctcgaccgc tgggagttcc
     2401 tgcgcgagct gcccgagaag cgggcgggcc tcggcggggt gccgagctgg ctgcgcatcc
     2461 ccaagctcga gtactcgctg ccgccggtcc tcgccatcgg cgcggtgctg ttcttcttct
     2521 tcctgcagaa ggacctaggg ccggcgctgg tgctctcgtt cctgttcctg ctcctctaca
     2581 gcgtcgcccg cggacggccg accatgatgg ccgtgggggc cgtcctggta gtggcggcgt
     2641 tcatggtcgg ctatcagctc ggctacccgc ggacggtgac cggccgcatc ggcatgtggc
     2701 tgtcgccgtg ggacaacgcg taccgcggcg gcgagcacct cgcgcagggg ctctgggcgc
     2761 tggccggcgg atcgctctcc ggcatggggc tggggctggg cgatccggag tacgtgccgg
     2821 agatccacac cgacatggtg ctggcggcgg tgggggagga gctgggcttc ctggggctcc
     2881 tggcggtctt cgccctctac gcactgctgg tctggcgcgg cctgcgcgcg gcgctgcgct
     2941 cgccgggcgt ctacggcttc ttcctggcgt tggggctgac gctgaccctc gccctgccga
     3001 tcctgctgat ctccggcggc gtcctcgggc tcgtgccgct gtcgggcgtg gtctcgccgt
     3061 tcctcagctt cgggcgctcg gccctgctcg ccaatctttt catcgccggc atgctgctgg
     3121 cggtctcggc gcggcgtttt cccggagggg gggcgaccga ggccgcggtg gtgcgccggt
     3181 tcgagggctc ggtgcgctgg atcggccttg gccttgccgc ggcgctggtg gtgatcctcg
     3241 gccggg
//
LOCUS       DASPJI010000300         3261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_77594, whole genome shotgun sequence.
ACCESSION   DASPJI010000300 DASPJI010000000
VERSION     DASPJI010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77594"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..756
                     /locus_tag="VEL74_07970"
     CDS             49..756
                     /locus_tag="VEL74_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007397136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYG62504.1"
                     /translation="MPAYNAEKTLERTYNDLPLDWVDDIILVDDGSRDRTVEISRRLG
                     IHTLVHPKNRGYGGNQKTCYREALSRGADIVVMVHPDHQYDPRYIPELVKPLLAEECD
                     AVFGSRMLGGRPIEGGMPKWKYFANLFLTGVANATFYVFLSEYHSGLRAYSRRYLEGV
                     DLEANSDDFVFDTEIIAQGVWAGMRIHEIPIQTRYFDEASQIGFTRSVRYGLEILRVL
                     TVYKLHKKGIRHSRLFR"
     gene            812..1417
                     /locus_tag="VEL74_07975"
     CDS             812..1417
                     /locus_tag="VEL74_07975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG62505.1"
                     /translation="MKALPPGATVVELGPGAAHVARLSGRSDLRWLGIESSLDCLPAL
                     SGILSGGVLADLEAQPRLPRCDAVLSGDTLEHMNDPEGMLRRIQEALPSGGLLLISVP
                     NVANAYVRLNLLFGRFPYADRGILDRTHRYFFTRASLRRMLEAHGFEVQRGAVSTIPL
                     PLALPGLPGPLLALLTGALSVATRLLPKLLGFQLLFVARKK"
     gene            complement(1522..1992)
                     /locus_tag="VEL74_07980"
     CDS             complement(1522..1992)
                     /locus_tag="VEL74_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62506.1"
                     /translation="MNKTLRLLSMASAFSVAALWLAPAPAAADGPFQFHSLTPCRVVN
                     TRNNTGTTGGAVASNATRSFTIQGACGVPAGAKAVALNVTIASPTNTGHLTLWPAGTT
                     LPTVSTINYNAGEPALANGAIVPLGTSTPDLSARPFLAGGGTVDLILDVTGYFM"
     gene            complement(2172..>3261)
                     /locus_tag="VEL74_07985"
     CDS             complement(2172..>3261)
                     /locus_tag="VEL74_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYG62507.1"
                     /translation="PPSVGDIDGDGEPEIVHGTGTYWPNRQERIYAWNCDGSAVSGWP
                     VTIQGQSMFSPALANLDGDPALEVVVAADNTRSSSTYHLYRFEGNGSQTFSAVPKDFF
                     GVSFGIGHPVIADVLGDATPEILTPTNGSVAVFSATGTLLTDDGSHPGGSTYAFYTET
                     TLSGVAVTDLNGDGKLEVLAISATPFPDATSTKINVWNPVNRTSAAPPWGFFRQDAAR
                     SGVAPGTGSCASACTLSSAATDFYTVTPCRVVNTRTTHTPALGNGQARTFTIAGSCGI
                     PATATAISVNVTVTQPTHTGHVRFGPGGCTQLPTSAINFGPGQTRANNAILPLANNGD
                     GTLTAIAAVLGSGSVHLLIDVNGYFQEP"
BASE COUNT          476 a         1098 c         1166 g          521 t
ORIGIN      
        1 cctcgcccgc agcccgcgcc cgcacggacc cggaagatct gtgtcgtgat gccggcctat
       61 aacgccgaga agaccctcga gcgcacctac aacgacctgc cgctcgattg ggtcgacgac
      121 atcattttgg tggacgacgg cagccgcgac cgcacggtgg agatctcgcg ccggctcggc
      181 atccacaccc tggtccaccc gaagaaccgg ggctacggcg gcaaccagaa gacctgctac
      241 cgcgaggccc tgtcccgcgg cgccgacatc gtggtcatgg tccaccccga ccaccagtac
      301 gacccgcgct acatcccgga gctggtcaag ccgctgctgg cggaggagtg cgacgccgtg
      361 ttcggcagcc gcatgctggg cggccgcccc atcgaggggg ggatgccgaa gtggaagtac
      421 ttcgccaacc tcttcctgac cggcgtcgcc aacgccacgt tctacgtctt cctctcggag
      481 taccactcgg ggctccgggc ctacagccgg cgctatctcg agggcgtgga cctggaggcc
      541 aactcggacg atttcgtgtt cgacaccgag atcatcgccc agggggtgtg ggcggggatg
      601 cgcatccacg agatcccgat ccagacccgc tacttcgacg aggcgtcgca gatcggcttc
      661 acccgcagcg tccgctacgg cctcgagatc ctgcgggtgc tcaccgtcta caagctgcac
      721 aagaagggga tccggcattc ccgactcttc cgctgagcgt tacgtcctca aggacttccc
      781 gggctcctcg caccgcatcc tggccggctg gatgaaggcc ctgccgccgg gtgcgacggt
      841 ggtggagctg ggcccggggg ccgcgcacgt ggcgcgcctc agcggccgct ccgacctgcg
      901 ctggctgggg atcgagagct cgctcgactg cctgccggcg ctctccggca tcctctcggg
      961 cggcgtgctg gccgacctcg aggcgcagcc gcggctgccg cgctgcgacg cggtgctctc
     1021 cggcgacacc ctggagcaca tgaacgatcc ggaggggatg ctgcggcgga tccaggaggc
     1081 gctgccctcc ggcgggctgc tgctcatctc ggtgcccaac gtggccaacg cctacgtgcg
     1141 gctgaacctg ctgttcggcc gcttcccgta cgccgaccgc ggcatcctgg accgcaccca
     1201 ccgctacttc ttcacccgtg cctcgttgcg gcggatgctg gaggcgcacg gcttcgaggt
     1261 ccagcgcggc gcggtgtcga ccatcccgct ccccctggcc ctccccgggc tgcccgggcc
     1321 gctcctcgct ctgctcaccg gcgccctgag cgtcgcgacg cggctcctgc cgaagctgct
     1381 gggatttcag cttctgttcg tagcccgcaa aaaatagcca tatggcgcct gaagcacgga
     1441 agccggagcc cgagggctcc ggcttcgcga ggaatcgcgc cggctcctgc tgcttcggcc
     1501 gtgtccgttc ccgtcagggg actacatgaa gtagccggtg acgtcgagga tcaggtcgac
     1561 cgtgccgccg ccggccagga aggggcgggc ggagaggtcc ggggtcgagg tgcccagcgg
     1621 cacgatggcg ccgttggcga gcgccggctc accggcgttg tagttgatcg tcgacaccgt
     1681 gggcagtgtg gtgcccgccg gccagagggt gaggtggccg gtgttcgtcg gcgaggcgat
     1741 ggtgacgttc agcgccaccg ccttggcgcc ggccggcacg ccgcaggcac cctggatcgt
     1801 gaaggagcgg gtggcgttgc tcgccaccgc gccacccgtg gtcccggtgt tgttccgcgt
     1861 gttgacgacg cggcacgggg tcaaggagtg gaactggaac gggccgtccg cagccgccgg
     1921 cgcgggcgcc agccacagcg cggcaacgga aaaggcggag gccatggaca ggagccggag
     1981 tgtcttgttc atttcacgat ccccatagag gtgttgtcat tctatccgaa agagccggtc
     2041 ggcgtcgaga cccactggag cccggccggg agcccggacc tccacgccgt cgatgtacct
     2101 gcctgaatga aagtccatcg tcattataca tccgtcatcc gccgaagagt gacaggagcg
     2161 cggattccat ttcacggctc ctgaaaatac ccgttgacgt cgatcagcag atggacgctc
     2221 cccgagccca ggaccgcggc gatggcggtc agcgtgccgt caccgttgtt ggccaggggg
     2281 aggatggcgt tgttggcccg cgtctgcccc gggccgaagt tgatggcgct ggtcgggagt
     2341 tgggtgcagc cgcccgggcc gaagcgcacg tgcccggtgt gggtcggctg ggtcacggtg
     2401 acgttgaccg agatcgccgt cgccgtggcc gggatgccgc acgagccggc gatggtgaac
     2461 gtccgcgcct ggccgttgcc cagggcgggc gtgtgcgtcg tccgggtgtt gaccacccgg
     2521 cagggcgtca cggtgtagaa gtccgtggcc gccgatgaca gggtacaggc cgaggcgcag
     2581 gagccggtcc ccggcgccac gccgctccgc gccgcgtcct gccggaagaa gccccacggc
     2641 ggcgccgcgc tggtgcggtt caccgggttc cagacgttga tcttggtgct cgtggcgtcc
     2701 gggaagggcg tcgcggagat ggcgagcacc tccagcttgc cgtcgccgtt caggtccgtc
     2761 accgccacgc ccgagagggt ggtctcggtg tagaaggcat aggtggaccc gccgggatgg
     2821 ctgccgtcgt cggtcagcag ggtgccggtg gcggagaaga ccgccacgct gccgttggtc
     2881 ggggtcagga tctcgggcgt cgcgtccccc aggacgtcgg cgatcaccgg gtggccgatg
     2941 ccgaaggaga cgccgaagaa gtccttgggc acggcggaga aggtctggct gccgttcccc
     3001 tcgaagcgat agagatggta ggtgctgctc gaccgggtgt tgtccgccgc cacgaccacc
     3061 tcgagggcgg ggtcgccgtc caggttggcg agggcggggg agaacatgct ctgcccctgg
     3121 atggtgaccg gccagccgga gaccgcggag ccgtcgcagt tccaggcgta gatccgctcc
     3181 tgccggttcg gccagtaggt gccggtgccg tggacgatct cgggctcgcc gtcgccgtcg
     3241 atgtcgccga cggacggcgg g
//
LOCUS       DASPJI010000301         3315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_361271, whole genome shotgun sequence.
ACCESSION   DASPJI010000301 DASPJI010000000
VERSION     DASPJI010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361271"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VEL74_07990"
     CDS             <1..455
                     /locus_tag="VEL74_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYG62508.1"
                     /translation="TAVSEEETVVRAIQTLAEDYILKPFNPREMVARVERVLRRIGDF
                     AYTLDPLIRVDDRLSVDFAHQKVLVEGTAVPLTSTESKLLYVLMRQAGRVVPTEFLIR
                     RLWPSEETFEDTLRVHIHRLRGKIEPDPARPRYVVTERGVGYRFPRPS"
     gene            complement(431..787)
                     /locus_tag="VEL74_07995"
     CDS             complement(431..787)
                     /locus_tag="VEL74_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HYG62509.1"
                     /translation="MTDIPGDAADEMRELQEQFARRLPERVAEIEEAWQAALGAGWEA
                     GRLRQLFRLAHSLRGAAATFGFADAAAIASRLESLLQEVTASGGPPDEEIARRIAGLV
                     DELRSQAPSQDGRGNR"
     gene            1032..2927
                     /locus_tag="VEL74_08000"
     CDS             1032..2927
                     /locus_tag="VEL74_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYG62510.1"
                     /translation="MDFNRDALDEQVLATHIVAARTAAARVEAFLATRGELAAGAAAD
                     PAYADPRSPAAQEALRGDLAAWAGLGVLGLAVVDERGEEAIRAQLRDDEAKRRTAAAL
                     RSPSREPVLALPGDGAAPATVRLEVPLSAGGGRLRLVCDGGPLAEAVSPGELGEGAEL
                     VLAGRDGMVLVGEANALAGFPRAMVAQATSGKLGGANRYDGGDGQRVLGAYSPVRGSG
                     WVVLSRQPGVVADAVARRMWRRSLLAVAAALLLILLLSAAAWVTVVRPIRELARAQGE
                     LVRAQGVLQGGGPGTVALEEAGGDEIGSLRRSFEALQRSLADRESLEGVSLGRYRVLE
                     YLGTGAMGTVFRGLDPRLRRPVALKTVRLGEHLDAERRHQLLETLLHEAVTVASLSHP
                     NVVAVYDLEDAPEGAFVAMELVEGENLDELLWRRGRLSAGQVIPLGAAIARGLAAAHA
                     RDIVHRDVKPANILLGRDGSIKVSDFGIADLVARTAQKGDRVFGTPGYMPPESLRGGG
                     HGKSGDLFAMGVILYECLSGTRPFDGANVAEVARETLFGEVRPLERQVSGLPPALVAL
                     VKSLLEKDPDLRPAGADVVAAELERMAAAEGLRWDPAAERAVVAAPPVREIKASQWVP
                     TVLIEPA"
BASE COUNT          397 a         1183 c         1274 g          461 t
ORIGIN      
        1 tgaccgcggt ctcggaggag gagacggtgg tccgtgccat ccagacgctg gccgaggact
       61 atatcctcaa gcccttcaac ccccgcgaga tggtggcccg ggtggagcgc gtgctgcgcc
      121 ggatcggcga cttcgcctac accctcgacc cgctgatccg ggtcgacgac cgcctgtcgg
      181 tcgacttcgc gcaccagaag gtgctggtgg aggggaccgc cgtgccgttg acctcgaccg
      241 agagcaagct gctctacgtc ctcatgcgcc aagcggggag ggtggtgccg acggagttcc
      301 tgatccggcg gctctggccc tcggaggaga cgttcgagga caccctgcgg gtccacatcc
      361 accggctgcg cggcaagatc gagcccgacc ctgcccgccc tcgctatgtg gtcaccgagc
      421 gcggcgtcgg ctaccggttc ccgcgtccgt cctgagacgg ggcctgggac cgcagctcgt
      481 ccacgagacc ggcgatgcgt cgcgcgatct cctcatccgg cggccctcct gacgccgtga
      541 cctcctggag cagggactcg aggcgcgagg cgatggcggc ggcgtccgcg aagccgaagg
      601 tggccgccgc gcctctcagc gaatgggcga gacggaagag ctggcggagc cgtccggcct
      661 cccagccggc gcccagcgcc gcctgccagg cctcttcgat ctccgccacc cgctccggca
      721 ggcggcgggc gaactgctcc tggagctcgc gcatctcatc ggcggcatct ccaggaatgt
      781 cggtcacgtc atggctcccc gggccggtcc ggccctgtcc aaaggcttca ctcggacgcc
      841 tgattagttt tttggatttt gcgttaaatc gctgaccgga ggcaagcatc tcttccaccg
      901 tccccgccgg cccggctgaa gcgaagccgg aaacgaagcc ccgccgctgg cggctcgggc
      961 tcctcggccg catcgccgcc gccctggccg cggtcggcct gctgccggta ggcgtcgcct
     1021 tcttcgggct ggtggacttc aaccgcgacg cgctggacga gcaggtgctg gccacgcaca
     1081 tcgtggcggc gcggacggcc gccgcacgcg tcgaggcgtt cctcgccacc cgcggcgagc
     1141 tggcggcggg cgcggcggcc gacccggcct acgccgatcc gcgctcgccc gcggcccagg
     1201 aggcgctgcg cggggatctc gcggcctggg cgggactcgg cgtgctcggc ctcgcggtgg
     1261 tggacgagcg cggcgaggag gcgatccggg cgcagctccg cgacgacgag gcgaagcggc
     1321 ggacggcggc ggcgctgcgc tccccctcgc gggagccggt gctcgccctg cccggcgacg
     1381 gcgccgcgcc ggcgaccgtg cggctggagg tgccgctgag cgccggcggc gggcgtctcc
     1441 gtctggtctg cgacggcggg ccgctcgccg aagcggtgag ccccggggag ctcggcgagg
     1501 gggccgagct ggtgctcgcc ggccgcgacg gcatggtcct ggtaggggag gcgaacgccc
     1561 tggccggctt cccgcgcgcc atggtggcgc aggcgacgtc cggcaaactg ggcggggcga
     1621 accgctacga cgggggggac gggcagcggg tgctgggggc ctactcgccg gtgcgcggca
     1681 gcggctgggt ggtgctctcc cggcagccgg gcgtggtggc cgacgccgtg gcgcggcgca
     1741 tgtggcggcg ctcgctcctc gccgtggccg cggcgctgct gctgatcctg ctgctctcgg
     1801 ccgccgcctg ggtgacggtg gtccgcccga tccgcgagct cgcccgcgcc cagggggagc
     1861 tggtccgcgc gcagggggtc ttgcaggggg ggggcccggg gacggtggcg ctggaggaag
     1921 ccgggggcga cgagatcggc tccctgcgcc gcagcttcga ggcgttgcag cgcagcctcg
     1981 ccgaccgcga gtcgctcgaa ggggtctcgc tgggccgcta tcgggtgctg gagtatctgg
     2041 gcaccggcgc catggggacg gtcttccggg ggttggaccc gcgcctgcgg cggccggtgg
     2101 cgctcaagac cgtgcggctg ggcgagcatc tggacgccga gcggcggcac cagctcctgg
     2161 agacgctgct ccacgaggcg gtcaccgtgg cgagcctcag ccatcccaac gtggtggcgg
     2221 tctacgacct ggaggacgcg ccggaggggg ccttcgtggc catggagctc gtcgaggggg
     2281 agaacctgga cgagctgctg tggcgccggg gccgcctgtc ggccggccag gtgatcccgc
     2341 tcggcgcggc gatcgcccgc ggcctggccg ccgcccacgc ccgggacatc gtccaccgcg
     2401 acgtgaagcc ggccaacatc ctcctcggcc gcgacggctc catcaaggtg tcggacttcg
     2461 gcatcgccga cctggtggcc cgcaccgccc agaaggggga ccgggtcttc ggcacgccgg
     2521 gctacatgcc gccggagagc ctgcgcggcg gcggccacgg caagagcggc gacctcttcg
     2581 ccatgggggt gatcctctac gagtgcctca gcggcacccg tcccttcgac ggcgccaacg
     2641 tcgccgaggt ggcgcgggag acgctgttcg gcgaggtgcg ccccctggag cgccaggtct
     2701 ccgggctgcc gccggccctg gtggcgctgg tcaagagcct gctggagaag gacccggatc
     2761 tgcgccccgc cggcgccgat gtcgtcgccg ccgagctgga gcggatggcg gctgcggagg
     2821 ggctgcgctg ggatccggcg gcggagcggg ccgtcgtggc cgccccgccc gtgcgggaga
     2881 tcaaggcctc ccaatgggtg ccgacggtgc tgatcgagcc ggcgtaggcc ctcacccccc
     2941 gaccccctct cccgcctccc cgccctcctt cccgggagag ggggaggcgg tttcctgctg
     3001 gcggagattg cgacggcgcg ttgcaagctt ccaacggctc ggcggagact ccgacagcgc
     3061 gtcggagctt ccggcggccc gccggagact ccgctggcgt gccggaagct tcggcagctc
     3121 gccgaagact ccgttggcgc gttggaagct tcggcagcgt gccggaagat tccggcagcc
     3181 cgccggagat tccgatgagc cgccggaacc ttcggcagct cgccggcgac tccgctggcc
     3241 cgttggaagc ttcggcagcg cgctggaagc ttccggcggc ccgccggaga ctccgctggc
     3301 gcgccggaat cttcg
//
LOCUS       DASPJI010000302         5560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_312426, whole genome shotgun sequence.
ACCESSION   DASPJI010000302 DASPJI010000000
VERSION     DASPJI010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312426"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1614)
                     /locus_tag="VEL74_08005"
     CDS             complement(<1..1614)
                     /locus_tag="VEL74_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYG62511.1"
                     /translation="MSWDGDAAALATLAADLRRSVRGEVRFDAGSRALYATDASNFRQ
                     VPLGVAVPLDIEDVERAVEVCRRHGAPLLGRGGGTSLAGQCCNTAVVLDLSRHLRRVL
                     EIDPGRRAARVEPGVILDDLRREAGRHGLTFGPDPATHAWCTLGGMIGNNSCGTHSLT
                     TGRTSDNVEELTVLTYDGLRLRVGATPEPELERIIRSGGRRGEIYRRLRDLRDRYGDL
                     IRSRFPAIPRRVSGFNLDELLPEKGFQVARALTGTEGTCVTVLEATVRLVQRPAARAL
                     LVLGYPDVFHAADHVLKILEEGPIGLEGMDRFLVENEHTRHAYREVLDRLPQGGAWLL
                     AEFGGEDPAEAADRARRVADRLSRRPDAPGLLLLEEPAAQARVWEVREAAVGTTSRDP
                     RLGDAWPGWEDSAVPPARLSSYLRELSALLGRHGYGAALYGHFGEGCLHARINFGLAD
                     EAGVRRYRAFMEEAADLVVAHGGSLSGEHGDGQQRGELLARMYGEELVQAFREFKAIW
                     DPQGRMNPGKVVDPASLDADLRLRVSLGEP"
     gene            complement(1611..2318)
                     /locus_tag="VEL74_08010"
     CDS             complement(1611..2318)
                     /locus_tag="VEL74_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62512.1"
                     /translation="MVLRKSSTTALATALTVCLLAGAAGAQDTGLVEGVPAPTLEQAI
                     PLFAWSPQGRSPALLFARFGTAERPAAGVMTSTRDGWRLVQLPDSIYYTVWSYVGRSS
                     GEQIWGISQPAGPQPATLEFSASSNGGRGWTHRSSLRKVSPYAVVDAFGMDAEGHGSV
                     VLRLDEDPTPNAPRLGFYMYLTKNGAKSWSEPIYSFERPRLSGDMPAAPDRSFAYDAP
                     PGVDAWRGILTSLAPSG"
     gene            2443..2868
                     /locus_tag="VEL74_08015"
     CDS             2443..2868
                     /locus_tag="VEL74_08015"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HYG62513.1"
                     /translation="MIPQTHSGIDPRLCGRPVVLDPGAARVELETVPEMAADETGLVH
                     GGFVFGLADYAAMLAVNHPNVVLGSAQTRFLKPVVVGERLVADASLLEEEGRKRRVAV
                     EVRRADQVGEMGELVMTGELLCFVPDRHVLAGPVREGGS"
     gene            2865..4619
                     /locus_tag="VEL74_08020"
     CDS             2865..4619
                     /locus_tag="VEL74_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HYG62514.1"
                     /translation="MNGGDLIAAVLEAQGVRFLFTLCGGHISPILVGAKQRGIRVVDV
                     RHEVDAVFAADAVYRLTGVPGVAAVTAGPGVTNTVTAVKNAQMAQSAVVLLGGAAATL
                     LKGRGALQDIDQLALLRPLVKWSATVNRVKDLAPLLERAFVEARAGVPGPVFLECPVD
                     LLYGEELVRTWYGAASKGGGLAGMAMRMYMDRHAGRLFFGADKAHARPPRPAPRVTPP
                     QPGQGEIRKAAARLRKARRPVLLVGSQAMLSPAEVGDLVLSIDALGVPVYLAGMARGL
                     LGPGHPLQLRHKRKEALREADLVILAGIPSDFRLDYGRHIGRRAYLIGANRSRTDLEL
                     NRKPDLSVHGDPAQFLRGLAGRLGGGGNLGQPGKRWADWLGTLRQRDKEREVEIDAQA
                     GEDIGLVNPIHLCREIDRVLSPDSLIVADGGDFVATAAYTLSPRSPLSWLDPGVFGTL
                     GVGGGFALGAKLCRPDADVWVIYGDGSVAYSLAEFDTFARHGLPVIAVVGNDAGWAQI
                     AREQIEILKDDVGTVLARTDYHQVAEGYGGRGLLISRPEEIRPSLEEALRLARAGSPV
                     LVNVQIGKTQFRKGSISI"
     gene            4708..>5560
                     /locus_tag="VEL74_08025"
     CDS             4708..>5560
                     /locus_tag="VEL74_08025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYG62515.1"
                     /translation="MNERRAARASLAAAVIAGLGLRLWNLRGQVMGGDELHAVRVAAR
                     RTVPEILATYRLTDVSIPLTALARWLMEQGLTLSELDFRLPALLCGIAALLVIPRAFL
                     GKMERMEVELLGWLVAFSPALVLYSRIARSYMPMVLAGFGAVMTFDRWWRTRSWKAGV
                     AYVVLAGLAVWLHLGAAPLVVAPFLFALGDLVPPRREGRAKGLLALVGLGAGLAAALA
                     LILVPARRSLAVLVAAKRQEQTVPLASVLDALHLQAGTESDLLAALFWTAALAGAILL
                     LRDRPRLG"
BASE COUNT          728 a         1968 c         2081 g          783 t
ORIGIN      
        1 tggctccccc agggagaccc gcagccggag gtccgcgtcc agcgaagcag ggtccaccac
       61 cttgcccggg ttcatccgcc cctggggatc ccagatcgcc ttgaattcgc ggaacgcctg
      121 gaccaactcc tcgccgtaca tgcgggcgag gagctcgccg cgctgctggc cgtctccgtg
      181 ctcgccggac agcgaccctc cgtgcgccac caccaggtcc gccgcctcct ccatgaacgc
      241 ccggtagcgg cgcaccccgg cctcgtccgc caggccgaag ttgatgcgcg cgtggaggca
      301 gccctccccg aagtggccgt agagggcggc gccgtagccg tggcggccga gcagcgccga
      361 cagctcgcgc agataggagg agagccgcgc cggcggcacc gccgagtcct cccagcccgg
      421 ccaggcatcc ccgagacgcg ggtcccgcga cgtggtgccc acggccgcct cccgcacctc
      481 ccagacccgc gcctgcgccg cgggctcctc cagcaggagc agacccggcg cgtccggccg
      541 ccgggagagg cggtcggcga cccggcgggc tcggtccgcc gcctcggccg gatcctcgcc
      601 gccgaactcg gcgagcagcc aggcgccgcc ctgggggaga cggtcgagga cctcgcggta
      661 ggcgtggcgg gtgtgctcgt tctccaccag gaagcggtcc atcccctcga gaccgatggg
      721 accctcctcc aggatcttca gcacgtgatc ggcggcgtgg aagacgtccg ggtagccgag
      781 gacgaggagc gcgcgagccg ccggccgctg gaccagccgg acggtagcct cgaggaccgt
      841 gacgcaggtg ccctcggtgc cggtgagcgc ccgcgcgacc tggaagccct tctcgggcag
      901 caactcgtcg aggttgaagc ccgagacccg ccgggggatc gccgggaagc ggctgcggat
      961 gagatcgccg tagcggtcgc ggaggtcccg cagccggcga tagatctcgc cgcgccggcc
     1021 gccggagcgg atgatcctct ccagctccgg ctcgggggtg gccccgacgc gcaggcgcag
     1081 gccgtcgtag gtgaggacgg tcagctcctc gacgttgtcc gaggtgcggc cggtggtgag
     1141 cgagtgggtg ccgcaggagt tgttgccgat catcccgccg agcgtgcacc aggcgtgggt
     1201 ggccgggtcg gggccgaagg tcaggccgtg ccgccccgcc tcccggcgga ggtcgtcgag
     1261 gatgacgccg ggctcgacgc gggcggcgcg gcggccgggg tcgatctcca gcacccgccg
     1321 cagatggcgc gacaggtcga gcaccacggc ggtgttacag cactggccgg cgaggctggt
     1381 gccgccgccc ctccccagca ggggagcgcc gtgccggcgg cagacctcga cggcgcgctc
     1441 cacgtcctcg atgtcgaggg ggaccgcgac gccgaggggg acctgccgga agttggaggc
     1501 gtcggtggcg tagagggcgc ggctgccggc gtcgaagcgg acctcgccgc gcaccgaccg
     1561 ccgcaggtcg gcggcaagcg tggccagcgc ggcggcgtcg ccgtcccagc tcaccccgag
     1621 ggggccaggc tcgttaagat gcctcgccag gcgtccaccc ccgggggggc gtcgtaggcg
     1681 aagctccggt ccggggccgc gggcatgtcc cccgacagcc gcgggcgctc gaacgagtag
     1741 atcggctccg accacgactt ggcgccgttc ttggtcaggt acatgtagaa gccgagccgg
     1801 ggggcgttcg gggtggggtc ctcgtccaga cggaggacca cggagccgtg cccctcggcg
     1861 tccatgccga aggcgtccac cacggcgtag ggggagacct tgcgcaacga agagcgatgg
     1921 gtccagccgc ggccgccgtt cgagctcgca gagaactcga gggtggccgg ctgcggtccg
     1981 gccggctggg agatccccca gatctgctcg ccggaggacc ggccgacgta gctccagacc
     2041 gtgtagtaga tcgagtcggg gagctggacc agccgccagc cgtcccgcgt cgaggtcatc
     2101 actccggccg cgggcctctc cgcggtgccg aagcgggcga agagcagggc gggcgaccgg
     2161 ccctgcgggc tccaggcgaa gaggggaatg gcctgctcca gagtcggcgc gggcacgcct
     2221 tcgacgagcc cggtatcctg ggcgccggcg gctccggcca gcagacagac cgtgagcgcg
     2281 gtggccagag cggtggtcga gctcttccgt aggaccatgg tcgtcctcct tcgaggtccg
     2341 gatgaagagt attccgtcat ccactccggg agagcccgga acagcgcgga tcggattcgc
     2401 ggcgacacca tgactataat cgttccggaa ggaactcgca ccctgattcc gcaaactcac
     2461 tccggcatcg acccccgtct ctgcggcaga cccgtcgtcc tggaccccgg ggcggcccgg
     2521 gtggagctcg agaccgtgcc cgagatggcg gccgacgaga ccgggctggt ccatggcgga
     2581 ttcgtcttcg ggctcgccga ctacgcggcc atgctggccg tgaaccaccc caacgtggtg
     2641 ctggggagcg cccagacgcg gttcctcaag cccgtggtgg tgggggagcg gctggtcgcc
     2701 gacgcgtcgc tgctggagga ggagggcagg aagcgccggg tcgccgtcga ggtgcggcgc
     2761 gctgaccagg tgggggagat gggggagttg gtgatgacag gtgagctcct ctgctttgtc
     2821 cctgaccggc acgtgctggc cggtccggtg cgggagggcg gttcatgaat ggtggtgacc
     2881 tgatcgcggc ggtcctcgaa gcccaggggg tgcggttcct cttcaccctc tgcggcggcc
     2941 acatctcccc catcctggtg ggggccaagc agcgcggcat ccgggtggtg gacgtccggc
     3001 acgaggtgga cgcggtcttc gccgccgacg ccgtctaccg gctgaccggc gtgcccggcg
     3061 tcgccgcggt caccgccggc ccgggtgtca ccaacaccgt caccgcggtc aagaacgcgc
     3121 agatggccca gtccgccgtc gtgctgctgg gcggcgccgc ggccaccctc ctcaaaggta
     3181 ggggggcgct ccaggacatc gaccagctcg ccctgctccg gccgctggtc aagtggtcgg
     3241 cgacggtgaa ccgggtgaag gacctggcgc ccctcctgga gcgcgccttc gtcgaggccc
     3301 gcgccggggt gccggggccg gtcttcctcg agtgtccggt ggacctcctc tatggcgagg
     3361 agctggtgcg gacctggtac ggcgccgcgt ccaagggggg cgggctggcg ggcatggcga
     3421 tgcggatgta catggaccgc cacgccggcc ggctcttctt cggcgccgac aaggcccacg
     3481 cccggccgcc gcggccggcg ccgcgcgtga cgccgccgca gccggggcag ggcgagatcc
     3541 gcaaggcggc cgcccggctc cggaaggccc gccggccggt gctgctggtc ggcagtcagg
     3601 ccatgctgtc acccgccgag gtgggtgatc tggtcctctc gatcgacgcc ctgggcgtcc
     3661 cggtctatct ggccggcatg gcccgcggcc tcctcggccc ggggcacccg ctccagctcc
     3721 gccacaagcg caaggaggcg ctacgcgagg ccgacctggt gatcctggcc ggcatcccca
     3781 gcgacttccg gttggactat gggcgccaca tcggccgccg tgcctacctg atcggcgcca
     3841 accgcagccg caccgacctg gagctgaacc gcaagcccga cctctccgtc cacggcgacc
     3901 cggcgcagtt cctccgcggc ctcgccgggc ggctgggcgg cgggggaaac ctgggccaac
     3961 ccggcaaacg ttgggcggac tggctgggca ccctgcgcca gcgcgacaag gagcgggagg
     4021 tggagatcga cgcccaggcg ggggaggaca tcggtctggt caatccgatc cacctctgcc
     4081 gggagatcga ccgggtactc tcgcccgaca gcctgatcgt ggccgacggc ggcgacttcg
     4141 tggccaccgc ggcctacacc ttgagcccgc ggagcccgct ttcgtggctc gaccccgggg
     4201 tgttcggcac cctcggcgtg ggcggcggct tcgccctggg cgccaagctc tgccggccgg
     4261 acgccgatgt ctgggtgatc tatggcgacg gctcggtggc ctacagcctg gccgagttcg
     4321 acaccttcgc ccgtcacggc ctgccggtga tcgccgtggt gggcaacgac gccggctggg
     4381 cccagatcgc ccgcgagcag atcgagatcc tgaaggacga cgtggggacg gtcctcgccc
     4441 gcaccgacta ccaccaggtg gccgaagggt acggcggccg tggcctgctg atctcccgcc
     4501 cggaggagat ccgcccgtcg ctggaggagg cactgcggct cgcccgcgcc ggctcgccgg
     4561 tgctggtcaa cgtccagatc ggcaagacgc agttccgcaa gggctccatc tccatctgac
     4621 gaaagggagg gagggggtag aaatgaccga ccgcttgcgc tccgttgtcg gcctgatcgt
     4681 ctgcagtggc aggtcgctct tcgtgggatg aacgaacgcc gcgccgccag ggcctccctc
     4741 gccgccgccg tgatcgcggg gctgggtctg cgcctgtgga acctgcgcgg ccaggtgatg
     4801 ggcggggacg agctgcacgc cgtgcgggtg gcggcccggc ggaccgtccc cgagatcctc
     4861 gccacctacc ggctgacgga cgtctcgatc cctctgaccg cgctggcgcg gtggctgatg
     4921 gagcaggggc tgaccctgtc cgagctggat ttccggctgc cggccctgct ctgcggcatc
     4981 gccgccctgc tggtgatccc gcgggcgttc ctggggaaga tggagcgcat ggaggtggag
     5041 ctcctgggct ggctcgtggc cttctcgccc gccctggtgc tctacagccg gatcgcccgc
     5101 tcctacatgc ccatggtgct ggccgggttc ggcgccgtca tgacgttcga ccgctggtgg
     5161 cgcacccgga gctggaaggc cggggtggcc tacgtcgtcc tcgccggcct cgcggtctgg
     5221 ctccacctgg gagcagctcc cctggtcgtg gcgcctttcc tcttcgcgct gggggatctg
     5281 gtgccgccgc ggcgcgaggg gagggccaag gggctgctgg ccctggtagg gctgggagcc
     5341 ggcctcgccg cggcgctggc gctcatcctg gtgccggcgc gccggtctct ggcggtgctg
     5401 gtcgccgcca agcgccagga gcagacggtc cccctggcga gcgtcctcga cgccctgcac
     5461 ctccaggccg ggacggagtc ggacctgctc gccgcccttt tctggactgc ggccctggcc
     5521 ggcgccatcc tcctgcttcg cgaccgtccg cggctggggg
//
LOCUS       DASPJI010000303         4647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_312433, whole genome shotgun sequence.
ACCESSION   DASPJI010000303 DASPJI010000000
VERSION     DASPJI010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312433"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VEL74_08030"
     CDS             complement(<1..888)
                     /locus_tag="VEL74_08030"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HYG62516.1"
                     /translation="MRRALPLLSLLPALLLLPALAAALDVPYLTGRVNDYANMLSPEQ
                     KQRIEQKLAGFEQRGGGAQVVVLTVPSLEGDAIEAFSVRAAQTWKLGREEKDNGVLLV
                     IAQQDRKMRVEVGYGLEPVLTDLESGIVLDEVIRPRFQAGDFGGGIEAGVDAILQALA
                     GELTPQRPATRLPTGGFTGGESAIMLLIFGLVVGVFSLVAIASPGCQGWFLYLFLTPF
                     YFIFPAMVWPMAGPILGIGWLVAFPILKTLASRLGFGRRWLPTGPAMGRRHRRGGGGW
                     IIGGGGFGGFGGGGFGGGGG"
     gene            complement(885..1547)
                     /locus_tag="VEL74_08035"
     CDS             complement(885..1547)
                     /locus_tag="VEL74_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62517.1"
                     /translation="MDPLHRLFTDADLEAVRAAVQEAEARTAGEIVPYVVEESDEYPT
                     AAWKGAALGALLGPLAAAVLFYAAGHWATHPPVWMLLPPAAGAAAGYLLTLLIAPVKR
                     WMAGEELLEVRSRRRAEVAFLEQEVFRTRDRTGILLFVSLFEHRVVVLADSGIHQKVA
                     PGEWDGIVAGVVQGIRAGRPGAALIAAIAECGKLLERHGVARRSDDVDELPNELRRER
                     PE"
     gene            complement(1573..3477)
                     /locus_tag="VEL74_08040"
     CDS             complement(1573..3477)
                     /locus_tag="VEL74_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYG62518.1"
                     /translation="MALPSGDGAGPRQDDRYRRIVETAHNGIWEIAADGATLYANPRM
                     GEMLGVTPEEMLGRSTFDFVFPEDVEKGMVYFALRRGGDRGTFKWRFRRADGSELWTL
                     ASCHPVLDADGSFIGAAGIFSDLTEQRLAEERLRASEERFRASVDTLLDCFGIYSAVR
                     DAQGRIADFRTEYLNDAACHNNRLPLEEQVGRTLLQLMPNHRSTGLFDDYCRVVETGE
                     PFSKETVFYEDDYAGEHLGRWFDIRANRLGDGFAVAWREVTERKRAETELRRQHDLLQ
                     AVLDSTSDIVFVKDTEGRYLLVNHALTEMAEKPAEEILGKPDGDLFPGESPERLRQVG
                     EDDRRAMALPEGELLRFEEEVVTPRGRRHCLTTKAAYHGADGRVLGVIGIVRDITERK
                     LVEDTLREADRRKDEFLAMLAHELRNPLAPIRNSVEILRLLGGEQPLMKQARDTIDRQ
                     VTHMSRLLDDLLDVSRVARGKIRLHQERCDLARIVWQTAEDYRGTLEAGGLHIGLRMP
                     AGPLWVTGDPVRLAQVVSNVLHNSGKFTEPGGAVTVTLEEAPDGVADLRIRDTGIGME
                     PEMVARAFDTFSQADSGLDRSRGGLGLGLALVKGLVELHGGTAAASSPGPGQGTEIAI
                     RLPLEPTPLG"
     gene            complement(3485..3904)
                     /gene="arfB"
                     /locus_tag="VEL74_08045"
     CDS             complement(3485..3904)
                     /gene="arfB"
                     /locus_tag="VEL74_08045"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HYG62519.1"
                     /translation="MVWIAEGLDIPEEELSFATARSGGPGGQNVNKLETKVTVRFDVA
                     GSPSLTDAQRQRLRERLATRITRDGVLQVSSQRHRSQGANRDAAVARFAELVQEALAE
                     EAPRRPTRPTAASRRKRLDQKRRQSRRKQQRAILPED"
     gene            3982..>4647
                     /locus_tag="VEL74_08050"
     CDS             3982..>4647
                     /locus_tag="VEL74_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYG62520.1"
                     /translation="MSTLRTTIRPRRPRLAALLLAFPLVAAAWSTPESVQSLGAPFSA
                     NDIPLRRDLDLQFVTTAPGSPTAISHTGDDRLFVTLRDGRIVIFRSGGFVAQPFLDIR
                     GQVSTQGEGGLLSTAFHPRYAENGLFFINYTNTAGDTVIARYQVSADRDRADTGSRRV
                     LLTIDQPFSNHNGGQMQFGPDGYLYIGMGDGGGAFDSQCNAQRDDTLLGKMLRLDVGQ
                     NVST"
BASE COUNT          641 a         1688 c         1662 g          656 t
ORIGIN      
        1 tcccccgccg ccgccgaatc caccgccgcc gaatccgccg aagcccccgc cgccgatgat
       61 ccagccgccg ccgccccggc ggtgccgccg ccccatcgcc gggcccgtgg gcagccagcg
      121 ccgcccgaag cccagacggg aggccagggt cttgaggatc gggaaggcga ccagccagcc
      181 gatgcccagg atggggccgg ccatcggcca gaccatggcg gggaagatga agtagaaggg
      241 ggtcaggaac aggtagagga accaaccctg gcaccccggc gaggcgatgg cgaccaggga
      301 gaacaccccc accaccagcc cgaagatgag cagcatgatg gccgactcgc cgccggtgaa
      361 gccgccggtg ggcaggcggg tcgccggccg ctgcggcgtc agctcgccgg ccagggcctg
      421 gaggatggcg tccaccccgg cctcgatgcc gccgccgaag tcgccggcct ggaagcgggg
      481 gcggatgacc tcgtcgagca cgatgccgct ctcgaggtcg gtgagcacgg gctcgaggcc
      541 atagcccacc tcgacgcgca tcttccggtc ctgctgggcg atgacgagca ggacgccgtt
      601 gtccttctcc tcccgtccca gcttccaggt ctgggcggcc cgcaccgaga aggcctcgat
      661 ggcgtccccc tccaggctgg ggacggtgag gacgaccacc tgggcgccgc cgccgcgctg
      721 ctcgaagccg gccagcttct gctcgatccg ctgcttctgc tcgggcgaca gcatgttggc
      781 gtagtcgttg acccggccgg tgaggtacgg gacgtcgagg gccgccgcca gagccggcag
      841 gagcagcagc gccggcagga gcgacaggag cggcagggcg cgcctcactc cggcctctcc
      901 cggcgcagct cgttgggcag ctcgtcgacg tcgtcggacc ggcgcgccac gccgtgccgc
      961 tccaggagct tgccgcactc ggcgatggcg gcgatcaggg cggcgcccgg gcggccggcg
     1021 cggatcccct ggaccacccc ggcgacgatg ccgtcccact cccccggcgc caccttctgg
     1081 tggatgccgg agtcggccag gacgacgacc cggtgctcga acagcgagac gaagaggagg
     1141 atgccggtgc ggtcgcgggt gcggaagacc tcctgctcca ggaaggcgac ctcggcccgc
     1201 cgccgggagc gcacctccag cagctcctcg cccgccatcc agcgcttgac cggcgcgatc
     1261 agcagggtga ggagataccc cgccgccgcc cccgccgcgg ggggcaggag catccagacc
     1321 ggcggatggg tggcccagtg ccccgccgcg tagaagagga cggcggcggc cagggggccg
     1381 aggagcgccc ccagcgccgc gcccttccag gcggccgtgg ggtattcgtc gctctcctcc
     1441 accacgtagg ggacgatctc ccccgccgtc cgggcctccg cctcctgcac ggcggcgcgc
     1501 acggcttcga ggtcggcatc ggtgaagaga cggtggagcg ggtccatggc cggcccgatg
     1561 gtatcacccg ggctatccca ggggcgtggg ctcgagcggc aggcggatgg cgatctccgt
     1621 cccctgcccg ggcccggggc tgctcgccgc ggcggtgccg ccgtggagct cgaccagccc
     1681 cttgaccagc gcgagcccga gccccaggcc gccgcggctc cggtcgagcc cgctgtcggc
     1741 ctggctgaac gtgtcgaacg cgcgggcgac catctccggc tccatgccga tgccggtgtc
     1801 gcggatgcgc aggtcggcca ccccatcggg cgcctcttcg agggtcacgg tgacggcgcc
     1861 gccgggctcg gtgaacttgc cggagttgtg cagcacgttg ctgacgacct gggccaggcg
     1921 gaccggatcg ccggtgaccc agagcgggcc ggccggcatc cggaggccga tatgcagccc
     1981 ccccgcttcc agggtgccgc ggtagtcctc ggccgtctgc cagacgatcc gggcgaggtc
     2041 gcagcgctcc tggtggaggc ggatcttgcc gcgggccacc cgggagacgt cgaggaggtc
     2101 gtccagcagg cgggacatgt gggtgacctg ccggtcgatc gtgtcgcgcg cctgcttcat
     2161 cagcggctgc tcgcccccga ggagccgcag gatctcgacc gagttgcgga tgggggccag
     2221 agggttgcgg agctcgtggg cgagcatggc caggaactcg tccttgcggc ggtccgcctc
     2281 gcgcagggtg tcctcgacga gcttgcgctc ggtgatgtcg cgcacgatcc cgatgacgcc
     2341 caagacccgc ccgtccgccc cgtgataggc ggccttggtg gtcaggcagt ggcgccggcc
     2401 gcggggggtg acgacctcct cctcgaaccg caggagctct ccttcgggga gggccatggc
     2461 ccgccggtcg tcctccccga cctgccgcag ccgctcgggc gactcacccg gaaacaggtc
     2521 gccgtccggc ttgccgagga tctcctccgc cggcttctcg gccatctcgg tgagggcgtg
     2581 gttgaccagg aggtagcggc cctcggtgtc cttgacgaac acgatgtccg aggtgctgtc
     2641 gaggaccgcc tggagcaggt cgtgctgccg ccgcagctcg gtctcggcgc gcttcctctc
     2701 ggtcacctcc cgccaggcga cggcgaaacc gtcccccagc cggttggccc ggatgtcgaa
     2761 ccagcggccg aggtgctcgc cggcgtagtc gtcctcatag aagaccgtct ccttgctgaa
     2821 cggctcgccg gtctccacca ctctgcagta atcgtcgaac agcccggtcg accggtggtt
     2881 cggcatcagc tgcaggagcg tccgcccgac ctgttcctcc agcggcaggc ggttgttgtg
     2941 gcaggcggcg tcgttgaggt actcggtccg gaagtcggcg atccggccct gcgcgtcgcg
     3001 caccgccgag tagatgccga agcagtcgag cagggtgtcg acggaggcgc ggaaccgctc
     3061 ctcggacgcc cgcagccgtt cctccgccag cctctgctcg gtgaggtcgg agaagatgcc
     3121 cgccgcgccg atgaagctcc cgtcggcgtc caggacgggg tggcaggagg cgagggtcca
     3181 gagctccgag ccgtcggcgc ggcggaaccg ccacttgaac gtgccccggt ctccgccgcg
     3241 gcgcagggcg aagtagacca tgcccttctc cacgtcctcc gggaagacga agtcgaacgt
     3301 ggagcggccg agcatctcct ccggggtcac cccgagcatt tcgcccatgc gggggttcgc
     3361 gtagagggtc gcgccgtcgg ccgcgatctc ccagatgccg ttgtgagccg tctcgacgat
     3421 ccggcgatag cggtcgtcct ggcgcgggcc ggcgccatca cccgagggga gtgccatggg
     3481 gggatcagtc ctccggcagg atagcccgct gctgcttgcg gcggctctgg cgacgtttct
     3541 ggtcgagccg cttccggcgg ctggcggcgg tgggccgggt gggacggcgc ggtgcctcct
     3601 cggccagggc ctcctgcacc agctcggcga agcgcgccac ggcggcgtca cggttggcgc
     3661 cctggctgcg gtggcgctgc gaggagacct ggaggacgcc gtcgcgggtg atgcgggtgg
     3721 cgaggcgctc gcgcaggcgc tgccgctggg cgtcggtcag gctcggggag ccggcgacgt
     3781 cgaagcggac ggtcaccttg gtctccagct tgttgacgtt ctgtcccccc gggccgccgc
     3841 tgcgcgcggt ggcgaagctc agctcctctt cggggatgtc gaggccctcc gcgatccaca
     3901 ccatgacctc aatggtatcc cgccggtgct actctgagcc gtcggccacg ccggccacgg
     3961 atcttttcga ggagcacgag catgagcacc ctgagaacga cgatccgtcc ccgccgtccc
     4021 agactggcgg cgctccttct ggccttcccc ctggtggcgg ccgcctggag cacgcccgaa
     4081 tccgtccagt cgctgggggc ccccttctcc gccaacgaca tccccctgcg gcgggacctc
     4141 gacctccagt tcgtgaccac cgcccccggc tcgccgacgg cgatcagcca caccggcgac
     4201 gaccggctgt tcgtcaccct ccgcgacggg cggatcgtca tcttccggag cggcggcttc
     4261 gtggcccagc cgttcctcga catccggggg caggtgagca cgcagggcga aggcggcctc
     4321 ctctcgaccg cgttccaccc ccgctatgcc gagaacggtc tcttcttcat caactacacc
     4381 aacacggccg gcgacacggt gatcgcccgc taccaggtgt ccgccgaccg cgaccgggcc
     4441 gacacgggga gccggcgggt gctgctgacc atcgaccagc cgttctccaa ccacaacggc
     4501 ggccagatgc agttcggccc cgacggctac ctctatatag ggatggggga cggcggcggc
     4561 gccttcgact cccagtgcaa cgcgcagcgc gacgacaccc tgctggggaa gatgctgcgc
     4621 ctcgacgtcg gccagaacgt ctccacc
//
LOCUS       DASPJI010000304        10827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_191840, whole genome shotgun sequence.
ACCESSION   DASPJI010000304 DASPJI010000000
VERSION     DASPJI010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10827)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10827
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191840"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VEL74_08055"
     CDS             complement(<1..237)
                     /locus_tag="VEL74_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HYG62521.1"
                     /translation="MADLKKAGSVALVGRPNAGKSTLMNRLLEEKLAIVSDKPQTTRH
                     RLVGILSGERGQMVFYDTPGMHRPLHRLNRQMVQA"
     gene            complement(237..1541)
                     /locus_tag="VEL74_08060"
     CDS             complement(237..1541)
                     /locus_tag="VEL74_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HYG62522.1"
                     /translation="MPEPAASLLPPFALGWIATLLLPLWVLVAVLSALLERSGPIRLR
                     HWAEEAGGRLRRLYDEPRHFGVFRYLLSLGSRLLPLCLVLTLAAILREYGTAHSGLWA
                     LGLVMLLVAVIEALNRTLVEHDPESALRWLTRAYRVALLLLWPLVLIVAPLVPARVLG
                     RREETEEDDEASDEEIEAFIDVGTREGILEPQEGEMLWSIVDFGDTQVRSVMTPRIDM
                     VCAPVDSSLESLADRFIDSGHSRIPLFEESIDKIVGILHIRDVLRASRAPEPGTAADL
                     MKPPLFIPETKPLGELLKELQARFQQVAIVVDEYGGTAGLVTVEDLVEEIVGEIMDEH
                     EALVAELEPLENGAYRLDGRAHIDLLDELFQVEIEDPEFETVAGLVFSALGRVPLVGE
                     SVDCYGLRFTVEAVADRRIQTVRVDRLSEEDAEAGDGREAEA"
     gene            complement(1534..2031)
                     /gene="ybeY"
                     /locus_tag="VEL74_08065"
     CDS             complement(1534..2031)
                     /gene="ybeY"
                     /locus_tag="VEL74_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HYG62523.1"
                     /translation="MKEPGQRGASGAGRGGGGVWDIVLQNPNRYPEAATRRLRPWLER
                     LVCSLVPVAAGEERSLGVRFSGDREMRRVNRGFRGKDKTTDVLSFPGEEEHVGDILIS
                     VPTARRQAAEAGHPVEREIRILLLHGLLHCMGYDHETDDGTMERLERKLRRSWIPAGE
                     AGGDA"
     gene            complement(2054..4432)
                     /locus_tag="VEL74_08070"
     CDS             complement(2054..4432)
                     /locus_tag="VEL74_08070"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HYG62524.1"
                     /translation="MDRDKGSPSRLQLLVDTGTANLRRRWRSRDLWRRALDTPALWVT
                     LLLVLGTWALMPGLLFAPRATPGAIADRDYVAPRDLLFKDEAASGAKQERARADVLPV
                     YDFDPGLTAERQRHLSQLFARGRQLLGAADDDPGAEPQEVRRRVVQELLRDEQARAGI
                     RLTSQEAELLARRRFAPELEERLAGVIAQALRRGVVSDKALLLENRMRGISLRNLATQ
                     RENVQVDLFDYLSYPDEVRDLVEAEVRDWSGLGAAERTLLVSLLVENIPANLYLNRSE
                     TLARQEAVAAEVGQVFNQIRKGQVIVRKGDVISAADAQAISQIRGERQLRLQVPPIAA
                     TFFLLGLACLVLWLGLASERVADHSRRRIFSEALLLLLLSVLGAKFCFLVAGALSGAF
                     EAAPLNSLRSYSYAIPFAALGLLAALLLGRNAALLLSILFSSLMSRLVVDGEPMWVVF
                     YAFAGSLTAIYALDLYQFRQRLVIARVGLMVGTINVVMALILTALTGLGDRGPVQIMF
                     DVVCGLAGGLLVAAAVSFALPILESLLAITTDIKLVELSNTNLPLLRRLAFEAPGTFQ
                     HSLMVANLAKEGCEAIGADTVLAYTAGLYHDVGKVLRPEYFIENQRPGRNPHDKLLPS
                     LSALILINHVKEGLELAREHGLPRVLLDAIQQHHGTRLIKYFYHRASEQREGTGEVVE
                     EKYRYPGPRPQNKVMGILMLADGVEAASRTLVEPTVPKIRGLIQTITDDCLREGQLDE
                     TDLTLSDLRKVSESFLRVLSNIFHHRIDYPGFDFNADPKREKRSATGAVKAS"
     gene            complement(4476..5495)
                     /locus_tag="VEL74_08075"
     CDS             complement(4476..5495)
                     /locus_tag="VEL74_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HYG62525.1"
                     /translation="MTQVTLAEEGLDVLFGTQDENLRRIERTFDVNLTARGNQILIKG
                     DDPARVQDVERLLYGLSQVNEKGYRFRPGEVQTAIRVAAEAPHASLVDFFLPEGLLAS
                     VRRLVSPRSFRQQLYLQAMADDDIVITIGPAGTGKTYLAVAMAAACLLEKRVRRIILA
                     RPAVEAGEKLGFLPGDLAEKVNPYLRPLHDALHDIIGYEKVARMMERGIIEVAPIAFM
                     RGRTLNDSFIILDEAQNTTTEQMKMFLTRIGFNSKAVVAGDVTQVDLPQGRMSGLREA
                     QEVLSGIEGIKFFTFDQRDVVRHNLVQKIVTAYDQHDRVKQEREAARPAVAGPPGQPG
                     GAPTP"
     gene            complement(5601..6458)
                     /locus_tag="VEL74_08080"
     CDS             complement(5601..6458)
                     /locus_tag="VEL74_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020139708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HYG62526.1"
                     /translation="MKIKKVGVLGCGLMGSGIAEVCARSGYDTVVREVSDELVEKGLG
                     KIRGSLDRAVEKGKLDAQARDEAAGRLSGAVDLHDLADCDLVVEAIIENLDEKKKTFA
                     VLDQVVKDGTIYASNTSSLTITQIAMSTKHPDRFVGLHFFNPVPVMKLVEVVRTLLTS
                     DDTFDSAFAFARSIGKEPIACRDNSGFIVNRLLVPYLLDAIRALEEGVGSVEDIDKGM
                     QLGCGYPMGPFTLLDFVGLDTTYYIANIMFEEYREKRFAPPPLLKQMVLAGRLGKKSG
                     RGFYEHGKK"
     gene            complement(6503..6910)
                     /gene="tsaE"
                     /locus_tag="VEL74_08085"
     CDS             complement(6503..6910)
                     /gene="tsaE"
                     /locus_tag="VEL74_08085"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HYG62527.1"
                     /translation="MSRNEEETLDLGVALAGELAPDGILLLSGDLGSGKTVLARGVAR
                     GLGIGPREVQSPTFTLVREHRGDGGTLIHADLYRLDPAEAASLGLEELLAGPGVKVVE
                     WAERLPFPVAGAVELRLNRLPDGSREIVQILQP"
     gene            complement(6924..8495)
                     /locus_tag="VEL74_08090"
     CDS             complement(6924..8495)
                     /locus_tag="VEL74_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HYG62528.1"
                     /translation="MRILTAEAMREVDRAAIEELGIPGLVLMENAAIGVVDAIGEVYP
                     EADSAAIFCGPGNNGGDGLAVARHLAARGYGVEVFLVSGRGRPVRLAGDAGTQLDICR
                     KLELPIRELAPGDGLAPALAVARESGLVVDALFGTGLGRPLEGIFAELVRALNELPVP
                     RVAVDLPSGLSGSRTDVFGPCLRADLTVTFAAPKLAHVFPPAAGWVGELIVTDLGFPP
                     ALIERVTEEGGPVHLLTAEELAGLVPGREAATHKGDYGHALIVAGSPGKAGAAILACR
                     SAVRVGTGLVTAAVPEPILDVVDLGSIESMTLPLPAGPAGQMAEEAAAAVLAAAEGKD
                     ALAMGPGLGNDPEGAAPAVRRIAAQCPLPLVLDADGLNAFAGRAAELAGRPGPTVLTP
                     HPGEMGRLLGIATAEVQADRIAAARRAAAATGAIAVLKGHLTLVAAPDGEVWVNPTGN
                     PGMASGGTGDVLTGLIAGLLAQGLDPLDAARLGVYLHGLAGDAAAARRGEPALAAGDL
                     IDALPEAFARLAGRE"
     gene            complement(8498..9184)
                     /locus_tag="VEL74_08095"
     CDS             complement(8498..9184)
                     /locus_tag="VEL74_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HYG62529.1"
                     /translation="MPTPPSPRLLLLFTKPARPGRVKTRLIGDLTPAEAAELHAAFLE
                     DLLERLRQGSFELRLAWALDPGEEIPAGPLPGIRQEGSGLGERLYRALSDAAATAESV
                     AAVGSDHPTLPLELVHRGFERVEAGADVVLGPAEDGGYYLIVIRAGVFTREAAPRLFE
                     EIAWSTERVLEQTLERCRELGLAVELLPTACDVDTPEDLRRLAGDLAGRAGGDCPRTR
                     GLLQSWRRGG"
     gene            9202..9984
                     /locus_tag="VEL74_08100"
     CDS             9202..9984
                     /locus_tag="VEL74_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HYG62530.1"
                     /translation="MALLTFLTDFGLTDYYVAAVKGTVLRLAPGTQIVDISHDVPPGD
                     IATGAFLLGAAVPSFPIGTVHLAVVDPGVGSRRRLLAARTPFGGWVGPDNGLFTPLLA
                     DARVHSVERAGLFLPGPGQTFHGRDRFAPIAAWLLRGEPIEELGPEVADPVRLTGDPP
                     RRSSGELRGRVIHVDRYGNLVTDIPAGWLPAEASGLRAEAGGHTATRFVAHYQQIPSG
                     EAALLSGSLGTLELSLNGADLSRAWGVARGQEVSVTWREDRK"
     gene            10129..10317
                     /locus_tag="VEL74_08105"
     CDS             10129..10317
                     /locus_tag="VEL74_08105"
                     /inference="COORDINATES: protein motif:HMM:NF025156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HYG62531.1"
                     /translation="MSKWEYKIINIRSENYRLDPNAATQLNQLGEDGWELVSITSVNF
                     KTGATDNIAMVFKRPVQG"
     gene            complement(10398..10634)
                     /locus_tag="VEL74_08110"
     CDS             complement(10398..10634)
                     /locus_tag="VEL74_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62532.1"
                     /translation="MSKELEEARRQVELFLEQRRREAEQRLTEVRTAVKTEVGEAPKK
                     AYALLLLAAGAGGFALAVRRRRRPRRARKVGKRR"
     gene            complement(10631..>10827)
                     /locus_tag="VEL74_08115"
     CDS             complement(10631..>10827)
                     /locus_tag="VEL74_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62533.1"
                     /translation="LGWLGAQRIRRVENPAASLKQRLDDHLDWWQHLLAETKALEVPA
                     AASSNRPTAAGGVGTERDLP"
BASE COUNT         1587 a         3886 c         3786 g         1568 t
ORIGIN      
        1 ggcctgcacc atctggcggt tcagccggtg gagcggccgg tgcatgcccg gggtgtcgta
       61 gaagaccatc tgcccgcgct cgccggagag gatccccacc agccggtggc gggtggtctg
      121 cggcttgtcg gagacgatgg ccagcttctc ctcgaggagc cggttcatca gcgtcgactt
      181 gcccgcgttg ggacggccca ccagggccac ggatcctgcc tttttcagat cggccatcag
      241 gcctccgcct ccctgccgtc gccggcttcg gcatcctctt cggagaggcg gtccacccgc
      301 acggtctgga tgcgccggtc cgccaccgcc tcgacggtga agcgcaggcc gtagcagtcg
      361 acgctctccc ccaccagcgg cacccggccc agggcggaga agacgaggcc ggcgaccgtc
      421 tcgaactccg ggtcctcgat ctccacctgg aacagctcgt ccagcagatc gatatgggcg
      481 cggccatcca gccggtaggc gccgttctcc agcggctcga gctcggccac cagggcctcg
      541 tgctcgtcca tgatctcgcc gacgatctcc tccaccaggt cctccacggt aacgaggccg
      601 gcggtgccgc cgtactcgtc gaccacgatc gccacctgct ggaaccgtgc ctggagctcc
      661 ttgagcagct cgcccagcgg cttggtctcg gggatgaaga gcggcggctt catcaggtcc
      721 gccgccgtcc ccggctcggg ggcccgcgag gcccgcagca cgtcgcggat gtggaggatg
      781 ccgacgatct tgtcgatcga ctcctcgaac agggggatgc gcgagtggcc ggagtcgatg
      841 aagcggtcgg ccaggctttc gaggctggaa tcgaccggcg cgcagaccat gtcgatgcgc
      901 ggggtcatga cgctgcgcac ctgggtgtcg ccgaagtcga cgatgctcca cagcatctcg
      961 ccttcctgcg gctcgaggat gccctcgcgg gtgccgacgt cgatgaaggc ctcgatctcc
     1021 tcgtccgagg cctcgtcgtc ctcctccgtc tcctcccggc ggccgaggac gcgggccgga
     1081 accagggggg ccacgatcag caccagcggc cagagtagga ggagggccac ccggtaggcg
     1141 cgggtcagcc agcgcagcgc cgactccggg tcgtgctcga ccagcgtccg gttgagcgct
     1201 tcgatgaccg cgaccagcag catcaccagc ccgagggccc acagcccgga gtgggcggtc
     1261 ccgtactcgc gcaggatcgc cgcgagggtc agcaccaggc agagcggcag caggcgcgag
     1321 cccagcgaga gcagataacg gaagactccg aagtggcggg gctcgtcata gagacggcgg
     1381 aggcgccccc cggcctcctc cgcccagtgg cggaggcgga tcgggccgct gcgctcgagg
     1441 agtgccgaga gcaccgccac cagcacccag agggggagca gcagcgtcgc gatccagccc
     1501 agggcgaagg gcggcagcag ggaggcggcc ggctcaggca tcgccgcccg cctctccggc
     1561 agggatccag gagcggcgga gcttgcgctc cagcctctcc atggtgccgt cgtcggtctc
     1621 gtggtcgtat cccatgcagt gcaggagccc gtgcaggagg aggatccgga tctcgcgctc
     1681 caccggatgg ccggcttcgg ccgcctgccg gcgcgccgtc ggaaccgaga tcaggatgtc
     1741 ccccacgtgc tcctcctcac ccgggaacga gagcacgtcc gtggtcttgt ccttgccccg
     1801 gaacccgcgg ttgacgcggc gcatctcgcg gtcgccggag aagcgcacgc ccaggctgcg
     1861 ctcctccccg gccgccacgg gcacgaggga gcataccagc cgctccagcc aggggcgaag
     1921 acgccgcgtc gccgcctcgg gatagcggtt cgggttctgc aggacgatgt cccagacgcc
     1981 tccgccaccc cgcccggcac cggaggcgcc ccgttgacca ggttctttca tcgcgtgaat
     2041 gccggccgcc ggctcatgag gccttcacgg ctcccgtcgc ggaccgcttc tcccgtttcg
     2101 ggtcggcgtt gaagtcgaag cccggatagt cgatccggtg atggaagatg ttggagagga
     2161 cccgcaggaa cgattccgac accttgcgca ggtccgacag cgtcaggtcg gtctcgtcga
     2221 gctgcccctc ccgcaggcag tcgtcggtga tggtctggat caggccgcgg atcttgggca
     2281 cggtgggctc gaccagggtg cggctcgccg cctcgacccc gtccgccagc atcaggatgc
     2341 ccatgacctt gttctgcggc cggggcccgg ggtaacggta cttctcctcc acgacctccc
     2401 ccgtcccctc ccgctgctca gaggcgcggt ggtagaagta cttgatcagc cgcgtgccgt
     2461 gatgctgctg gatggcgtcg aggagcaccc gcggcagccc gtgctcgcgc gccagctcca
     2521 gcccctcctt gacgtggttg atcaggatca gggcggagag cgacggcagc agcttgtcgt
     2581 gcgggttgcg gcccggccgc tggttctcga tgaagtactc ggggcggagc accttgccga
     2641 cgtcgtggta gaggccggcg gtataggcca gcaccgtatc ggcgccgatc gcctcgcacc
     2701 cctccttggc caggttggcg accatcaggg agtgctggaa cgtccccggc gcctcgaagg
     2761 cgaggcggcg cagcagcggc aggttggtgt tggacagctc caccagcttg atgtcggtgg
     2821 tgatggcgag gagcgactcg aggatcggca gggcgaagct caccgcggcc gccaccagca
     2881 ggccgccggc caggccgcag accacgtcga acatgatctg caccggaccg cggtcgccga
     2941 ggccggtgag cgccgtgagg atcagcgcca tgaccacgtt gatcgtcccc accatcagcc
     3001 cgacccgggc gatcaccagg cgctgccgga actgatagag gtccagcgcg tagatcgcag
     3061 tcagcgagcc ggcgaaggca tagaacacca cccacatcgg ctcgccgtcc accaccaggc
     3121 ggctcatcag gctggagaag aggatggaga gcagcagcgc cgcgttgcgg cccagcagca
     3181 gggcggcgag cagccccagg gcggcgaacg ggatggcgta cgagtagctg cgcagcgagt
     3241 tcaagggggc ggcctcgaac gcccccgaca gcgccccggc caccaggaag cagaacttgg
     3301 cccccagcac cgacagcagc agcagcagca gggcctcgga gaagatccgc cggcggctgt
     3361 ggtcggccac ccgctcggac gccaggccca gccagagcac caggcaggcc aacccgagca
     3421 ggaagaaggt ggcggcgatc ggcggcacct ggagccggag ctgccgctcg ccgcggatct
     3481 gggagatcgc ctgcgcgtcg gccgcgctga tcacgtcccc cttgcggacg atgacctgcc
     3541 ccttgcggat ctggttgaag acctgcccca cctcggcggc taccgcctcc tggcgggcca
     3601 gggtctcgct acggttgagg tacagattgg ccggaatgtt ctccaccaga agagacacca
     3661 ggagggtgcg ctccgccgcg ccgaggccgc tccagtcccg cacctcggcc tcgaccaggt
     3721 cccgcacctc gtccggatag ctcaggtagt cgaagaggtc cacctgcacg ttctcgcgct
     3781 gggtggcgag gttgcgcagg gagatgccgc gcatccggtt ctcgaggagc agcgccttgt
     3841 cgctgaccac gccgcggcgc agggcctggg cgatcacgcc ggcgaggcgc tcctcgagct
     3901 cgggggcgaa ccggcggcgc gccaggagct cggcctcttg agaggtgagg cggatgccgg
     3961 cgcgggcctg ctcgtcgcgc aggagctcct gcaccacccg ccggcggacc tcctgcggct
     4021 cggcgccggg atcgtcgtcc gccgccccca ggagctggcg cccgcgggcg aagagctggc
     4081 tcagatggcg ctggcgctcg gcggtcagcc cggggtcgaa gtcgtagacc ggcaggacgt
     4141 cggctcgggc cctctcctgt ttggccccgg acgcggcctc gtccttgaac agcaggtccc
     4201 ggggcgccac gtagtcgcgg tcggcgatgg ccccgggggt agcccggggg gcgaagagga
     4261 ggccgggcat cagggcccag gtgcccagca ccaggagcag ggtcacccag agggcgggcg
     4321 tgtccagggc ccggcgccag aggtcacgcg agcgccagcg gcgccgcaga ttggcggtgc
     4381 cggtatcgac gaggagctga aggcgtgatg gagagccctt gtctcgatcc atggcgaatc
     4441 ccgcgcgccg gaaagaccgg cgcgcgggcg ggggctcacg gagtcggagc ccctccgggc
     4501 tgtcccggcg gtcccgcgac ggccggccgt gccgcttcgc gctcctgctt gacgcggtcg
     4561 tgctggtcgt aggcggtgac gatcttctgc accaggttgt gacgcacgac atcccgctga
     4621 tcgaaggtga agaacttgat cccctcgatc ccggaaagga cctcctgggc ctcccgcagg
     4681 cccgacatcc ggccctgcgg caggtccacc tgggtgacgt cgccggccac cacggccttg
     4741 gagttgaagc cgatacgggt caggaacatc ttcatctgct ccgtcgtggt gttctgcgcc
     4801 tcgtccagga tgatgaagct gtcgttcagg gtgcgcccgc gcatgaacgc gatgggcgcc
     4861 acctcgatga tccctctctc catcatccgt gccaccttct cgtagccgat gatgtcatgg
     4921 agggcatcgt ggagcggccg gaggtagggg ttgaccttct cggccaggtc gcccggcagg
     4981 aagccgagct tctcgcccgc ctccaccgcc ggccgggcca ggatgatgcg ccgcacccgt
     5041 ttctccagca ggcaggcggc ggccatggcc acggccaggt aggtcttgcc cgtgcccgcc
     5101 gggccgatgg tgatgacgat gtcgtcgtcg gccatggcct gcaggtagag ctgctgccgg
     5161 aagctgcgcg gcgacaccag ccgccgcacc gaggcgagca gcccctcggg caggaagaag
     5221 tccaccagcg aggcgtgggg ggcctcggcg gcgacacgga tggcggtctg cacctcaccg
     5281 gggcggaaac ggtagccctt ctcgttgacc tgcgagaggc cgtagaggag ccgctccacg
     5341 tcctgcaccc gggcggggtc gtcgcccttg atcaggatct ggttgccccg ggcggtgagg
     5401 ttgacgtcga aggtgcgctc gatgcgccgg agattctcgt cctgggtgcc gaagaggacg
     5461 tcgaggcctt cctcggcgag agtcacttgc gtcattgctg agcttttctt gccgccgcgg
     5521 agacaggggg cggctctcca aggcgtccta gtgtagcaga ggcgggcggg ccacaccggc
     5581 ccgccgcctc tccctaccct ctacttcttg ccgtgctcgt agaagccccg gccgctcttc
     5641 ttgcccaggc ggccggcgag gaccatctgc ttgaggagcg gcggcggggc gaaacgcttc
     5701 tcccggtact cctcgaacat aatgttggcg atgtagtagg tggtgtcgag gccgacgaag
     5761 tcgagcaggg tgaaggggcc catgggatag ccgcagccga gctgcattcc cttgtcgatg
     5821 tcctccaccg agcccacccc ctcctccagg gcgcggatgg cgtccagcag gtagggcacc
     5881 agcaggcggt tgacgatgaa gcccgagttg tcgcggcagg cgatgggctc ctttccgatc
     5941 gagcgggcga aggcgaaggc gctgtcgaac gtgtcgtccg aggtgaggag cgtccgcacc
     6001 acctccacca gcttcatcac cggcaccggg ttgaagaagt gcagcccgac gaagcggtcg
     6061 gggtgcttcg tcgacatggc gatctgggtg atggtcagcg acgaggtgtt gctggcgtag
     6121 atggtgccgt ccttgaccac ctggtccagc acggcaaagg tcttcttctt ctcgtcgagg
     6181 ttctcgatga tcgcctcgac caccaggtcg cagtccgcca ggtcgtggag gtccacggcg
     6241 ccgctcaacc gccccgcggc ctcgtcccgg gcctgggcgt ccagcttgcc cttctccacc
     6301 gcccggtcga gggagccgcg gatcttgccc agccccttct ccaccagctc gtcgctcacc
     6361 tcgcgcacca cggtgtcata gcccgagcgc gcgcacacct cggcgatccc cgagcccatc
     6421 agcccgcagc cgaggacacc caccttcttg atcttcatgg gatgtatccc tcctctatcg
     6481 tcattcgtcg ttgtttcttc gttcacggtt gtaagatctg cacgatctcc cggctcccat
     6541 cgggcaaccg gttcagccgc agctccaccg ccccggcgac cgggaacggc agccgctccg
     6601 cccattccac cactttgacc cccggcccgg cgagcagctc ctcaaggccg agggaggccg
     6661 cctcggccgg gtccagccgg tagaggtcgg cgtgaatcag ggtgccgccg tccccccggt
     6721 gctcgcggac cagggtgaag gtcggagact ggacctcgcg cgggccgatc cccagcccgc
     6781 gggccacgcc gcgggcgagc acggtcttgc cgctccccag gtcgcccgag agcaggagga
     6841 tgccgtccgg cgccagctcc ccggcgagcg ccacgccgag gtccagcgtc tcctcctcgt
     6901 tccggctcac ccagcgccgc atctcattca cggcccgcga gacgggcgaa ggcttccggc
     6961 agggcgtcga tcaggtcgcc ggcggcgagg gcgggctcgc cccggcgtgc cgcggccgcg
     7021 tcgccggcca ggccatggag atagacgccg agccgcgccg cgtccagagg gtcgagcccc
     7081 tgggccagga gaccggcgat gagaccggtc aggacgtcgc cggtgccgcc gctcgccatg
     7141 cccgggttgc ccgtgggatt cacccagacc tccccgtccg gcgccgccac cagggtcaga
     7201 tgccccttga gcacggcaat ggcgccggtc gcggcggcgg cgcggcgcgc cgcggcgatg
     7261 cggtccgcct gcacctcggc ggtggcgatc cccagcagcc tgcccatctc gccggggtgg
     7321 ggggtcagca ccgtgggccc gggccgcccg gcgagctcgg ccgcgcggcc ggcgaaggcg
     7381 ttgagtccgt cggcgtccag caccagcggc agcgggcact gggccgcgat ccgccggacc
     7441 gcgggggccg ccccctccgg atcattcccc aggcccggcc ccatcgccag agcgtccttg
     7501 ccctccgccg ccgccagcac ggcggcggcc gcctcttcgg ccatctgccc ggcgggaccg
     7561 gcggggagcg gcagggtcat cgactcgatg gagcccaggt ccaccacgtc gaggatcggc
     7621 tccggcaccg cggcggtcac cagccccgtc cccacccgca cggcggagcg gcaggccagg
     7681 atggcggctc cggccttgcc cggggagccg gcgacgatca gcgcgtgccc gtagtccccc
     7741 ttgtgggtgg cggcctcgcg gcccgggacc agccccgcga gctcctcggc ggtcagcaga
     7801 tggaccggtc ccccctcctc cgtcacccgt tcgatcaacg ccggagggaa ccccaggtcg
     7861 gtgacgatca gctcgcccac ccagccggca gcgggtggaa agacgtgcgc cagcttcggc
     7921 gcggcgaagg tgaccgtgag gtccgcccgc aggcaggggc cgaagacgtc ggtccggctg
     7981 ccggagaggc cgctgggcag gtcgacggcg acccgcggca ccggcagctc gttcagggcc
     8041 ctcaccagct cggcgaagat cccctccaga ggacggccga ggccggtgcc gaagagggcg
     8101 tccaccacca ggccggactc ccgcgccacc gccagggccg gcgcgaggcc gtcgcccggc
     8161 gccagctccc ggatcggcag ctccagcttg cggcagatgt cgagctgggt gccggcgtcc
     8221 cccgcgagcc ggaccggccg gccgcgcccg ctcaccagga acacctcgac gccgtagccc
     8281 cgtgccgcca gatggcgcgc cacggcgagg ccgtcgccgc cgttgttgcc cgggccacag
     8341 aagatggccg ccgagtcggc ctccggatag acctcgccga tggcgtccac gacgccgatc
     8401 gcggcgttct ccatcagcac caggcccggg atgcccagct cttcgatggc cgcgcggtcc
     8461 acctcgcgca tggcttcggc ggtcaggatc cgcatggtca gcctcctcgc cgccaggact
     8521 gcagcagccc gcgggtgcgg gggcagtccc ccccggccct gccggccagg tcgccggcca
     8581 gccggcggag atcctccggc gtgtccacgt cgcaggccgt ggggaggagc tcgacggcga
     8641 gcccgagctc gcggcagcgc tccagggtct gctccaggac ccgctccgtg ctccaggcga
     8701 tctcctcgaa caggcgcggc gccgcctccc tggtaaaaac gcctgcccgg atcacgatga
     8761 gatagtagcc gccgtcctcc gccgggccga gcaccacgtc ggccccggcc tccacccgct
     8821 cgaagccgcg gtgcaccagc tccaggggca gcgtcgggtg gtcgctgccc actgcggcga
     8881 cggactcggc ggtcgcggcg gcatcagaca gggcgcggta cagccgctcg cccaggccgc
     8941 tcccctcctg ccggatgccc ggcaggggac ccgccgggat ctcctccccg ggatccagcg
     9001 cccaggcgag gcgcagctcg aagctcccct gccgcagccg ctccaggaga tcctccagaa
     9061 aggcggcgtg cagctcggcc gcctcggccg gggtaagatc cccgatcagc cgggtcttga
     9121 cccggcctgg gcgcgccggc ttggtgaaga gcaggagaag gcggggggag ggcggtgtcg
     9181 gcacgcgcgg attctagccc aatggccctt ctcaccttcc tgaccgactt cggcctgacc
     9241 gactactacg tcgccgccgt caaggggacg gtgctgcggc tcgcccccgg gacgcagatc
     9301 gtggacatct cccacgacgt gccgccgggc gacatcgcca ccggcgcctt cctcctcggc
     9361 gcggccgtgc cgtcctttcc catcgggacg gtccacctgg cggtggtgga ccccggcgtc
     9421 ggcagccggc ggcgcctgct cgccgcccga accccgttcg gcgggtgggt ggggccggac
     9481 aacggcctct tcacccccct cctcgccgac gcccgggtcc actcggtgga gcgggccggc
     9541 ctcttcctcc ccgggccggg ccagaccttc cacggccgcg accgcttcgc gccgatcgcg
     9601 gcctggctgt tgcgggggga gcccatcgaa gagctggggc cggaagtcgc cgacccggta
     9661 cggctcacgg gggatccacc tcggcgctcc tccggtgagc tgcgcggccg ggtgatccac
     9721 gtcgaccgct acggcaacct ggtcaccgat atacccgccg gctggctccc cgcggaggct
     9781 tccggcctgc gcgccgaggc gggcggccat acagccaccc gattcgtggc acactatcag
     9841 cagataccct caggcgaggc ggccctgctt tcagggtctc ttggaacgct ggagctctcc
     9901 ctcaatggcg cggacctctc ccgcgcctgg ggggtggcgc ggggccagga ggtaagcgtc
     9961 acctggcgcg aagatcgcaa gtagttttga ctgtggagcc cggtgtgacg gtacggttac
    10021 acaccgttta cagtcccgtt acacaggcgg cgggaatccg gctagaatag gcacgctttt
    10081 gcgaggtcga tcgggctgat gggggatctt cgggaaggag cgagtggaat gagcaagtgg
    10141 gagtacaaga tcatcaacat ccggagtgag aattaccgcc tcgaccccaa tgccgcgacg
    10201 cagttgaacc agttgggtga ggacggctgg gagctggtct cgatcacctc ggtgaacttc
    10261 aagaccggcg cgacggacaa catcgcgatg gtgttcaagc gccccgtcca ggggtaggca
    10321 gcagccagca gccgggtcgg cagtgttcgc tgccggcccc gtcaaagtcc ccaatcccga
    10381 cgacatccag gttcaggcta gcggcgcttg cccacttttc gggcacggcg aggacggcgg
    10441 cgcctgcgca ccgccagcgc gaagccgccg gcgccggccg ccagcaggag gagggcgtac
    10501 gccttcttcg gcgcctcccc cacctcggtc ttgaccgcgg tccgcacttc ggtgaggcgc
    10561 tgctcggcct cccgccggcg ctgctccaga aaaagctcga cctggcgccg cgcctcttcc
    10621 agctccttgc tcatggcaga tccctctccg tccccacgcc gccggcggcg gtgggacggt
    10681 tggaagaggc ggcggccggc acctccagcg ccttggtctc ggcgaggagg tgctgccacc
    10741 agtccaaatg atcgtcgagc cgctgcttca ggctggcggc ggggttctcg acccgtcgga
    10801 tgcgctgggc gccgagccat ccgagga
//
LOCUS       DASPJI010000305        17398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_762890, whole genome shotgun sequence.
ACCESSION   DASPJI010000305 DASPJI010000000
VERSION     DASPJI010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17398)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17398
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762890"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1269
                     /locus_tag="VEL74_08120"
     CDS             115..1269
                     /locus_tag="VEL74_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62534.1"
                     /translation="MAKTRPQSRERPREKPRQVIQVGHLQIELPEGVTALDWALERVN
                     YQNPRIRAFLGCIRLIEDVLESNYALLHCSPERLLDVWHKVRQVAKLIRTRLGPLLEV
                     PSRLPALEEARQSAEVALHLLDDHVLRELDRFPEEVAPEQILDVRKLLCVATGQIHSF
                     LLDTFGEIMAKDPRSLHDSDYFLSRRFPRDIEEAEWLHATVLRLQTYLEGMEPMRLQH
                     LAGLARRLRDEESLPPRATWEETSVFLQLLLDDLTPKLKGVLALRGVRFYEMEILDRY
                     TQEIPIRCRLLMELYATGTGAINDIKQRVGSSQKEREQNVADLLALHGAFSRRMAELM
                     SEIDQRLRDLLAFIPLWLEGIEKRRALLLRRSTRDLPAPDVPDESSETAG"
     gene            1364..2884
                     /locus_tag="VEL74_08125"
     CDS             1364..2884
                     /locus_tag="VEL74_08125"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYG62535.1"
                     /translation="MYEQRERFSKALAACCVLATLAGVASAREPLPEPQRPRTLVISL
                     DEALRIAAGESETVWAAEAGVLQAVGSRRIARSAWFPQVSADASYVRTLRSQFQDLDF
                     GGLGGGEPGEEPAIDLPFGRENQYSLGLSLSQLVFDGGQTAARNRAAAARLRSAELDV
                     TSAAAQTLLDVTQAYFDAQLADRLVQIAEASLEQSEETLRQTEVARRVGDKSEYELLR
                     ARVARDNQIPTVVRQRLQSTESYLRLKQLLNVPPGDQLVLTTGVEEEVPRFATAADVS
                     PDERAPVVQARENVEANEAQLSEARGQRLPAVSLSSRFAPVAYPASGLPEPDDFREDW
                     SVTLSLSVPLLTWGRLRGNEEVARGGLSQAQARLAQVREAAVLDATIARDDLARARAV
                     LAGNTSTVQEAERAFGIARIRFREGIATQIELDDARLLFEQAQVNRAIALRDVQAARA
                     RLALLADLPLSQGSGAPQLIQIQQQPVTQPSPSQPFATAATGSAVGIVPGTGGSFP"
     gene            2881..4026
                     /locus_tag="VEL74_08130"
     CDS             2881..4026
                     /locus_tag="VEL74_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYG62536.1"
                     /translation="MSSKPYLKNIGLSLGLAILAVLSYACGGREKEAAAPPPPVVLGP
                     ADTAVVVMAPIETGPALTGTLAAEEEATVRAQLGGTVLETYAEPGQAVRRGAALARLD
                     TAALRDAMASAQAAVTNARNSLAVAEREAERQRVLVQAGAVAERNVETAHQQVVAARA
                     ALSLAQAQLANAEEQVANTRITAPFSGVVSDKQVSAGDVVQPGAALFTIVNPSILELE
                     ATVPAERLSALRAGAPVQFNVNGYPGRRFTGEITRINPSADPVTRQVRVYAQIANPDG
                     ELVAGLFAEGRVATESRTALTVPRDAVDRRMAQRALLVVRQNRVGRVDVELGLEDEQE
                     QRVEIRRGAVQAGDVVLLGAAQGITPGTQVQLAPTVQERADRLAQAL"
     gene            4023..7196
                     /locus_tag="VEL74_08135"
     CDS             4023..7196
                     /locus_tag="VEL74_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYG62537.1"
                     /translation="MTISDFAIRRPIVTVVTMLALVIFGLFALFQLEVDEFPDLTNPL
                     VVVAVPYPGASPSQVEREVVDPMEEAFSALSGIDQITSTSLDGFGQITVQFVFSKSPD
                     QASQDVRDAIDGIRNDLPPEMEEPILRRFDPADIPIVSLVLTSQTMSPPELTLIADPG
                     ITRKLRGINGVAQVTVVGGVEREISVDVRPADLAAAGVSVAQVVQAVQGQNLAAPVGR
                     VEGKLTEEAIRLRGRLETPEQFEQLVVTSRNGQLIRLGQVADVRDGTSEQRSLALYNG
                     RPAVAIDITKSKGASTTTVADSVVTALTGIEKTLPQGVKLEVVRNSGERVRDSVTNVE
                     EALIEGAILTILVVFLFLNSWRSTVITGLALPVSVLSSFIAVWAFGFTLNTMSLLGLS
                     LAIGILIDDAIVVRENIVRHIEMGKDHKRAAHEGTNEIALAVAATTFSIVAVFVPVAF
                     MEGVAGQWFKPFALTIACAVLVSLFVSFSLDPMLSAYWPDPQIEAHERKNPIARALDW
                     FNQWFDRQADRYKGIVAWALDHRWTMVAIAAIAFFGAIGLQIAFGGGGFIPEGDYSEI
                     NLALETPPGSNLEYSAIKAQEISAVARAHPEVAYTYTSIGSADGSGGVDEGSIYIRLT
                     PKAEREVSQQELARQIRQEVRRIGGVTAYLSEGGPGGPGRQIQLQLRGQDMAALEKAA
                     QMMQAEIAKVPGATDIGLSNRGQKPELEIQLNRGLAGTLGLTVGQVAQSLRPAFAGID
                     AGDWVDPAGETRDVTVRLAASARQNLTDIEQLPLVVAGAPPVSGTGGAVGMSPPITVP
                     LGQLATVRRSVGPAQIEHLDRDKVITVGANVEGQSLSEVSQAINSRVKGLRLPPGVAL
                     SQGGEVESQNEVFTKVFAALALAVLLMYLILVVQFGSFLDPLAILLSLPLSLVGVVLI
                     LILTGDTLNIMSLIGVILLMGIVAKNAILLIDFAKWNHEKGMPLREALIEAGRVRLRP
                     IIMTTMALIAGMIPVALGVGEGADFRAPLGRAVIGGTITSTFLTLLVIPTVYEIMVEW
                     REKLRGLIRRRRRVDVQRPAEAAGPGH"
     gene            7253..7918
                     /gene="hxpB"
                     /locus_tag="VEL74_08140"
     CDS             7253..7918
                     /gene="hxpB"
                     /locus_tag="VEL74_08140"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016350848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HYG62538.1"
                     /translation="MPRALIFDMDGVLIDSEPFWREAEMEGFLLAGVRLEEKDCLETV
                     GLRIDEVVRLWYGRHPWSSPSPEEIERAILDRLVARVRERGTAKPGVREALAFARSAG
                     LRVALASSSPYRVIEAVVETLGLAADLEVLVSAEGEEWGKPHPAVYLNAARRLGIAPE
                     ECAAVEDSPNGVLSAKAARMKCIAVPEDTLRGHPYFAIADRVIGSLAELDAGLWQEVW
                     AAR"
     gene            7959..8594
                     /locus_tag="VEL74_08145"
     CDS             7959..8594
                     /locus_tag="VEL74_08145"
                     /inference="COORDINATES: protein motif:HMM:NF025724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4410 domain-containing protein"
                     /protein_id="HYG62539.1"
                     /translation="MKAPLRSLPLFALALLIASLPAAAAKEKKALTAPGTYIDWQDEI
                     DDLEIIESFKLADYSRVVVEPFDTSNTPLPEEADNAYQPVRKVLADATSPFVTSLREE
                     LKGKVEVAEGEKGSAASIGAEEKTLVVRGRVTTMDPGSRAARYWAGFGAGAARTGIAA
                     EVVDGATGKVLLRFQQERRSGVGAAGGSYENLLERNLRKIGEDAALILGAF"
     gene            8595..9389
                     /locus_tag="VEL74_08150"
     CDS             8595..9389
                     /locus_tag="VEL74_08150"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HYG62540.1"
                     /translation="MAGFDGKVALVTGGNSGIGRATALAFARQGARVVIAARRTEPGE
                     EVAHAIHQAGGEAVFVRADVARAEDVEALVRRTVEVYGPLDCALNNAAIEGEPRTLAD
                     YSEEEYDELMGVDLKGVWLCMKHQIRQMLAQSPSGGTIVNTSSVNGLGGVAHGSLYAA
                     AKAGVLALTKSAAQELGGQGIRVNALVAGGFRTPMLQRVFARHGGPDPEAQAAVEGQL
                     SQLIPLGRIGNPEEAADAVLWLCSDASSYMTGNSLILDGGLTAYYR"
     gene            9518..9757
                     /locus_tag="VEL74_08155"
     CDS             9518..9757
                     /locus_tag="VEL74_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62541.1"
                     /translation="MPTRILEFRRPDQQGFTRVGRVRTDGSIAFQGKVYRTIREVPQE
                     CLALRPDLETHSQWVRLYRAIDPKRPRREADRPDK"
     gene            9782..11029
                     /locus_tag="VEL74_08160"
     CDS             9782..11029
                     /locus_tag="VEL74_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015956719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG62542.1"
                     /translation="MARIVLTTFGSLGDLHPMIALALGLRERGHEPVFATAEYYRPKI
                     EELGFELRALRPHMTPDDPELAALVMDAKKGPENVLRGLIFPALRDTFEDLRRAAEGA
                     DFLVAGELIYGAQVLGELTGLPWANALLSPISFFSEYDPPVPTPYPGMAKLLGKARWI
                     DRGIVRFGRFVTRSWGQPVHDLRRELGLPRGGHPIFDKYSPHLVLALFSRLIGRPQPD
                     WPAHTEQTGFVFYDGRPGGARLTPELERFLDAGEPPLVFTLGSAAVLDAGDFYLESAD
                     AARQLGHRAVLLVGANPPPPDLPPEILAVDYAPFSDLFPRAAVIVHQGGIGTCAQALR
                     AGRPTLVMPYSHDQPDNAVRLERLGTSRTVPRDYYSAPRAAWELRELLADSKYRQKAE
                     EAGRIVSGEDGLTEACDAIEKHL"
     gene            complement(11263..12132)
                     /locus_tag="VEL74_08165"
     CDS             complement(11263..12132)
                     /locus_tag="VEL74_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013962170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HYG62543.1"
                     /translation="MQFAAIDVETANADLSSICQIGVAIFRDGSHVDSWSSLVDPEDD
                     FDGFNVSLHGIDECKVRGAPTWPRIAENLRALMAGSVVACHTPFDRAATALACERYGI
                     DQIDCRWLDTARVARRAWPDKFGAKGYGLYNVTEFLGIEFKHHDALEDARAAGEVLCK
                     ACACADLNLEGWFARVQRPINPACDQPIVRDGNNEGPLYGNVAVFTGSLAIPRREAAD
                     LAARAGCDVASSVTKKTTLLIVGDQDISKLAGKPKSSKHIKAEELIAQGCQIRILRES
                     DFQSIVVAQAVYG"
     gene            complement(12397..12675)
                     /locus_tag="VEL74_08170"
     CDS             complement(12397..12675)
                     /locus_tag="VEL74_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62544.1"
                     /translation="MHKLSQAGWSKDKVQVLSGEDIASIVKPKSDSEVFLSLHYPELK
                     DLGEHFLVTISAVLAFSVTFSEKIVGISGAGLIQRFLLLSSWGLLMLR"
     gene            13263..13862
                     /locus_tag="VEL74_08175"
     CDS             13263..13862
                     /locus_tag="VEL74_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62545.1"
                     /translation="MNSFTQGSTVSTHSPLPNAFKPTFLGRLAERDEPPTAGDADVAG
                     PWRIEPIPGQGFGLFRVGESLARGFAPTAVFPSRWLALLMAGILPGTGKDPLLLLSQQ
                     PDAHGVYPVALAGGGDDGGDDGDGRDGRGPGGRVPVGFLQLFDERAVDALNVVIHLLQ
                     APECLAFVLEAAGGVTLERCGAILDERVAAADAGGAAAG"
     gene            complement(14799..15794)
                     /locus_tag="VEL74_08180"
     CDS             complement(14799..15794)
                     /locus_tag="VEL74_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HYG62546.1"
                     /translation="MLIRQTAPVFGAASRLVPEKGEWQVSLSYRDLDSDTHYSGSERQ
                     RQRETLGTYVINTQQALDLGISYAWSDRLSFALGIPVIKASWAVPTPISPSPGPRAKQ
                     EASGLGDISLTGRYWVFAPESRHNVALGLGVKAPTGEYDVTQRYPNIDGTNDTVKAVD
                     QSVQPGDGGWGILFDVQAFRQYSRATLFASGTYLANPRDTNGTPSILVGLGLGNNPVF
                     AQEGTLVNSVPDQYLVRLGAVAPLGQSGFGASLAYRVEGLPRYDLIGDSHGWRRPGYE
                     MFVEPGIAYTKGASTWSLNVPIGFYRNREPNPYSGREGDATFPDYILLTGLSYRF"
     gene            complement(15928..>17398)
                     /locus_tag="VEL74_08185"
     CDS             complement(15928..>17398)
                     /locus_tag="VEL74_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG62547.1"
                     /translation="SERAAATQKKAQVTALSRSFSDLARLMGSVMGRKYVIYLSEGFD
                     SSVITGITDQAEIDQINESMTSGEFYRVDPSARYGNTEASNDLEKMLEELRRADCVIQ
                     SVDIGGLRGDDMNPQRRSGQDGLFLMAHDTGGELYRNFNDLGAAMDQMLQRTSVTYVL
                     TLQPEDLPRDGSYRRLKVTLKDAPRGTRVVHRPGYYAPKPFSQQTGGEKILTAASRIL
                     SGEDGGAIPLAVLAAPFRQPAGDQAYVPVLIEVDGKGLLAGHQGAAVPAEIFVYALDA
                     QGAVHDFQTQSVGLDVGKTGETLRRTGLKFFGHVDLAPGDYSLRVLVRNGVTGAYGMR
                     VVPLTVPAFAQAGPVLLPPLFPEPAGRWVLVPEAQREGEKRPDYPFMLAGEPFIPANR
                     PVLAPKQEARLELVGWNLGDGDLKATARVTSADGKEVPGSALSLGQRDSGAGGAAPDR
                     LLATFRPGELPPGEYRLAVQLTGPTGSVTSAPLRFRVER"
BASE COUNT         2689 a         6188 c         5836 g         2685 t
ORIGIN      
        1 ccgaggcggt cccggaagaa gcgctgaaga gggccgagag agcaccgccg ccaccctgcg
       61 gggtagcacc gctccggagg gtgtgtgcta gcgtctaggc tggaaccgtc cacgatggca
      121 aagacgaggc cccagtcgcg ggagagacca cgggagaagc cgaggcaggt gattcaggtg
      181 gggcatcttc agatcgagct gcccgaaggg gtgacggctc tggactgggc gctagagcgg
      241 gtcaactacc agaacccgcg tatccgcgcc ttcctcggct gcatccgcct gatcgaggac
      301 gtcctcgaga gcaattacgc gctcctccac tgctcgcccg agcggctgct ggacgtctgg
      361 cacaaggtcc gccaagtcgc gaagctcatc cgcacccgcc tcggcccgct gctggaggtg
      421 ccgtcgcggc tcccggcgct ggaggaggcg cgccagagcg ccgaggtcgc cctccacctc
      481 ctcgacgacc acgtgctgcg cgagctcgac cgcttccccg aagaggtggc ccccgagcag
      541 atcctcgacg tccgcaagct cctctgcgtc gccaccgggc agatccactc cttcctgctc
      601 gacaccttcg gcgagatcat ggccaaggac ccgcggagcc tgcacgactc cgactacttc
      661 ctctcgcggc gcttcccgcg cgacatcgaa gaggccgagt ggctccacgc cacggtgctg
      721 cgcctccaga cctatctcga ggggatggag ccgatgcgcc tccagcacct ggcgggcctc
      781 gcccggcgcc tgcgcgacga ggagtcgctc cccccgcgcg ccacctggga ggagacgtcg
      841 gtcttcctcc agctcctcct cgacgacctc acccccaagc tcaagggggt cctcgccctg
      901 cggggcgtgc gcttctacga gatggagatc ctggaccgct acacccagga gatccccatc
      961 cgctgccggc tgctcatgga gctgtacgcc accgggaccg gcgccatcaa cgacatcaag
     1021 cagcgggtgg ggagctcgca gaaggagcgc gagcagaacg tcgccgacct cctcgccctg
     1081 cacggagcct tcagccgccg catggccgag ctgatgtccg agatcgacca gcgcctgcgc
     1141 gacctcctcg ccttcatccc cctctggctc gagggaatcg agaagcgccg cgccctgctc
     1201 ctgcggcgga gcacccggga cctccccgca cccgacgtcc ccgacgagag cagcgaaacc
     1261 gcgggctgat cccgcccccc tggcatcagg ttcgcaactc acccggccgg gtgagaggtg
     1321 cgcggtgtgt tcccgccgac gtgccgtaga tcgggacggt tccatgtacg agcagagaga
     1381 aaggttttca aaggcgctgg cggcatgctg cgtactggcg actctggccg gagtggcctc
     1441 cgcccgggag ccgttgccgg agccgcagcg gccccgtacc ttggtgatct ccctggacga
     1501 ggcgctgcgc atcgccgccg gcgagagcga gaccgtgtgg gcggccgagg ccggcgtgct
     1561 ccaggcggtg ggcagccggc ggatcgcccg cagcgcctgg ttcccccagg tctcggccga
     1621 cgcctcctat gtccggacgc tacggtcaca attccaggac ctcgacttcg gcggtctcgg
     1681 cggcggcgag cccggcgagg agccggcgat cgacctcccc ttcggccgcg agaaccagta
     1741 ctcgctcggc ctgtcgctca gccagctcgt cttcgacggc ggccagaccg cggcgcgcaa
     1801 ccgggcggcg gcggcgcgcc tgcgctccgc cgagctcgac gtcacctcgg cggcggctca
     1861 gaccctcctc gacgtcaccc aggcctactt cgacgcccag ctcgccgacc gcctggtgca
     1921 gatcgccgag gcgtcgctgg agcagtccga ggagaccctg cggcagaccg aggtcgcccg
     1981 ccgggtgggc gacaagtccg agtacgagct gctgcgggcg cgggtggcgc gggacaacca
     2041 gatccccacc gtcgtccgcc agcgcctgca gagcaccgaa tcctatctgc gcctgaagca
     2101 gctcctcaac gtgccgcccg gggaccaact ggtcctcacc accggcgtcg aggaggaggt
     2161 gccgcgcttc gccacggctg ccgacgtctc gccggacgag cgggcgccgg tcgtccaggc
     2221 gcgggagaac gtcgaggcca acgaggccca gctctccgag gcccgcggcc agcggctccc
     2281 cgccgtctcc ctgtcgtcgc gcttcgcccc cgtggcctat ccggcgagcg gcctgcccga
     2341 gcccgacgac ttccgggagg actggtcggt taccctgagc ctctccgtcc ccctgctcac
     2401 ctgggggcgg ctgcgcggca acgaggaggt ggcccgcggc ggcctgtcgc aggcccaggc
     2461 ccgcctcgcc caggtgcgcg aggccgcggt gctcgacgcc accatcgccc gcgacgacct
     2521 cgcccgcgcc cgggccgtgc tggcgggcaa caccagcacc gtgcaggagg ccgagcgcgc
     2581 cttcggcatc gcccgcatcc gcttccgcga gggcatcgcc acccagatcg agctggacga
     2641 cgcccgcctg ctgttcgagc aggcgcaggt gaaccgcgcc atcgccctgc gcgacgtgca
     2701 ggcggcccgc gcccgcctgg cgctgctggc cgacttgccg ctctcccagg ggagcggggc
     2761 gccgcagctc atccagatcc agcagcagcc ggtgactcaa ccatcaccct cacagccgtt
     2821 cgccaccgcc gccaccggca gcgcggtggg gatcgttccg ggaaccggag gttccttccc
     2881 atgagctcca agccgtatct gaagaacata gggttgtccc tcggcctggc gatcctcgcc
     2941 gtgttgtcct acgcctgcgg tggccgcgag aaggaggccg ccgcgccgcc gccccccgtc
     3001 gtcctcggac cggcggacac ggccgtggtc gtcatggccc cgatcgagac cggcccggcc
     3061 ctgaccggca ccctggcggc ggaggaggag gccaccgtcc gcgcccagct cggcggcacc
     3121 gtccttgaga cctatgccga gcccggacag gcggtccgcc gcggtgccgc gctcgcccgc
     3181 ctcgacaccg ccgcgctacg cgacgccatg gcctccgccc aggcggcggt caccaacgcc
     3241 cgcaacagtc tcgccgtcgc cgagcgcgag gcggagcgcc agcgcgtcct cgtccaggcc
     3301 ggcgccgtgg ccgaacgcaa cgtcgagacc gcccaccagc aggtggtcgc cgcccgcgcc
     3361 gccctctccc tggcccaggc gcagctcgcc aacgccgagg agcaggtcgc caacacccgt
     3421 attaccgcgc ccttctcggg cgtggtcagc gacaagcagg tgtcggcggg cgacgtggtc
     3481 cagcccggcg ccgccctgtt caccatcgtc aacccgtcga tcctcgagct cgaggccacg
     3541 gtgccggcgg agcgcctctc cgccctgcgc gccggcgcgc cggtgcagtt caacgtcaac
     3601 ggctaccccg gccgccggtt cactggcgag atcacccgca tcaacccgag tgccgacccg
     3661 gtgacgcggc aggtgcgggt ctacgcccag atcgccaatc cggacggcga gttggtggcc
     3721 ggcctcttcg ccgagggccg ggtcgccacc gagtcgcgca ccgccctgac cgtcccccgc
     3781 gatgccgtcg accgccgcat ggcccagcgc gccttgctgg tggtgcgcca gaaccgcgtc
     3841 ggccgcgtcg acgtcgagct ggggctcgag gacgagcagg agcagcgggt cgagatccgc
     3901 cgcggcgcgg tgcaggccgg cgacgtcgtc ctcctcggcg cggcgcaggg gatcaccccc
     3961 ggcacccagg tgcaactggc tcccaccgtc caggagaggg ccgacaggct ggcccaggcg
     4021 ttatgacgat ctctgatttc gcgatccggc gccccatcgt caccgtggtg acgatgctgg
     4081 cgctggtgat cttcggtctc ttcgccctct tccaactgga ggtcgacgag ttccccgacc
     4141 tgaccaaccc gctggtggtg gtggcggtcc cttatcccgg agcctcgccg tcgcaggtgg
     4201 agcgcgaggt ggtggacccc atggaggagg cgttcagcgc cctctcgggg atcgaccaga
     4261 tcacctccac ctcgctcgac ggcttcggcc agatcaccgt gcagttcgtc ttctccaaga
     4321 gccccgacca ggcgagccag gacgtgcgcg acgccatcga cggcatccgc aacgacctgc
     4381 cgccggagat ggaggagccg atcctgcgcc gcttcgatcc ggcggacatc cccatcgtct
     4441 ccctggtcct cacctcgcag acgatgagcc cgccggagct gacgctgatc gccgacccgg
     4501 ggatcacccg caagctgcgc ggcatcaacg gggtcgccca ggtcaccgtc gtgggcggcg
     4561 tcgagcgcga gatctcggtc gacgtgcggc cagccgacct ggcggcggcc ggggtgagcg
     4621 tcgcccaggt ggtgcaggcg gtccaggggc agaacctggc ggcgccggtc ggccgggtgg
     4681 aggggaaact caccgaggag gcgatccgcc tgcgcggccg cctcgagact ccggagcagt
     4741 tcgagcagct cgtcgtcacc tcgcgcaacg gccagctcat ccgtctcggc caagtggccg
     4801 acgtgcgcga cggcacctcc gagcagcggt cactcgccct ctacaacggc cgcccggccg
     4861 tcgccatcga catcaccaag agcaaggggg cgagcaccac caccgtcgcc gactccgtgg
     4921 tcaccgcgct aaccgggatc gagaagacgc tgccccaggg ggtcaagctg gaagtggtga
     4981 ggaactccgg cgagcgggta cgcgactcgg tgaccaacgt cgaggaggcg ctcatcgagg
     5041 gggcgatcct caccatcctg gtggtcttcc tgttcctcaa ctcctggcgc tcgacggtga
     5101 tcaccggcct ggcgctgccg gtgtcggtgc tctcctcgtt catcgcggtc tgggccttcg
     5161 gcttcaccct gaacaccatg tcgctcctcg gcctgtcgct cgccatcggc atcctgatcg
     5221 acgatgccat cgtggtgcgg gaaaacatcg tgcgccacat cgagatgggg aaggaccaca
     5281 agcgggccgc ccacgagggg acgaacgaga tcgccctggc ggtggcggcc acgaccttct
     5341 cgatcgtcgc ggtgttcgtg ccggtcgcct tcatggaggg ggtcgccggg cagtggttca
     5401 agccgttcgc cctgaccatc gcctgcgccg tgctggtgtc gctcttcgtc tccttctccc
     5461 tggacccgat gctctccgcc tactggccgg acccgcagat cgaggcgcac gagaggaaga
     5521 accccatcgc ccgcgccctc gactggttca atcaatggtt cgaccgccag gcggaccgct
     5581 acaaggggat cgtcgcctgg gcgctggacc accgctggac catggtcgcc atcgccgcca
     5641 tcgccttctt cggcgccatc ggcctgcaga tcgccttcgg cggcggcggc ttcatccccg
     5701 agggggacta ctcggagatc aacctggcgc tggagacgcc gccgggctcg aacctggagt
     5761 actcggcgat caaggcgcag gagatctcgg cggtggctcg ggctcatccc gaggtggcct
     5821 acacctatac ctctatcggc agcgccgacg gctccggcgg cgtcgacgag gggagcatct
     5881 acatccgcct gacccccaag gcggagcggg aggtgtcgca gcaggagctg gcgcggcaga
     5941 tccgccagga ggtgcggcgg atcggcggcg tcacggccta cctttccgaa ggaggacccg
     6001 gcgggccggg ccggcagatc cagctccagc ttcgcggcca ggacatggcg gcgctggaga
     6061 aggccgccca gatgatgcag gcggagatcg ccaaggtgcc gggggcgacg gacatcggcc
     6121 tctcgaaccg gggccagaaa ccggagctgg agatccagct caaccgcggc ctcgccggca
     6181 cgctcggcct gaccgtcggc caggtggcgc agtcgctgcg cccggccttc gccggtatcg
     6241 acgccggcga ctgggtcgat ccggcgggcg agacgcgcga cgtcaccgtc cgcctcgccg
     6301 cctcggcgcg ccagaacctg accgacatcg agcagctccc gctggtggtg gcaggggcgc
     6361 ctcccgtctc cgggaccggc ggtgccgtgg gcatgagccc ccccatcacg gtccccctgg
     6421 gccagttggc gacggtccgg cggtcggtgg ggccggcgca gatcgagcac ctggaccgcg
     6481 acaaggtgat caccgtcggc gccaacgtcg aggggcagtc gctgtcggag gtgtcgcagg
     6541 cgatcaactc ccgggtgaag gggctgcggc tgcccccggg ggtcgccctg tcgcagggtg
     6601 gcgaggtgga gagccagaac gaggtcttca ccaaagtctt cgcggcgctc gccctggcgg
     6661 tgctgctgat gtacctgatc ctggtggtcc agttcggctc gttcctcgac ccgctggcga
     6721 tcctgctgtc gctgccgctg tcgctggtgg gcgtggtgct gatcctcatc ctcacgggcg
     6781 acacgctgaa catcatgagc ctgatcggcg tcatcctgct gatgggcatc gtggcgaaga
     6841 acgccatcct gctcatcgac ttcgccaagt ggaaccacga gaaggggatg ccgctgcgcg
     6901 aggcgctcat cgaggccggc cgggtgcggc tgcgcccgat catcatgacg acgatggcgc
     6961 tgatcgccgg catgatcccg gtggccctgg gcgtaggcga gggggccgac ttccgggcgc
     7021 cgctcggccg cgcggtcatc ggcggcacca tcacctcgac gttcctgacc ctgctggtga
     7081 tccccaccgt ctacgagatc atggtggagt ggcgcgaaaa gctccgcggc ctcatccggc
     7141 ggcgccgccg cgtggacgtc cagcggccgg cggaggcggc ggggccgggg cactgaacac
     7201 tcccttcatc ccgttagaat cctccggccg tcgctcggag acggccggac ccatgcccag
     7261 agccctcatc ttcgacatgg acggagtcct catcgactcc gagcccttct ggcgcgaggc
     7321 cgagatggag ggattcctcc tcgccggcgt ccgcctggag gagaaggact gcctggagac
     7381 cgtgggtctg cggatcgacg aggtggtccg cctctggtat gggcggcacc cgtggagctc
     7441 cccctctccc gaggagatcg agcgggcgat cctcgaccgg ctggtggcgc gggtgcggga
     7501 gcgcggcacg gccaagccgg gggtgcgcga ggcgctcgcg ttcgcccgga gcgccggcct
     7561 ccgcgtcgcc ttggcgtcgt cctcccccta ccgggtgatc gaagcggtgg tggagacgct
     7621 cgggctggcg gcggacctcg aggtcctcgt gtcggccgag ggggaggagt ggggcaagcc
     7681 ccacccggcc gtctatctca acgcggcgcg gcggctcggg atcgctcccg aagaatgcgc
     7741 ggcggtcgag gactccccga acggcgtgct gtcggccaag gcggcgcgga tgaagtgcat
     7801 cgccgtgccg gaggacaccc tgcgcggtca tccctacttc gccattgccg accgggtgat
     7861 cgggtcgctg gcggagctgg acgccggcct ctggcaggag gtctgggcgg cgcgataagc
     7921 tggaggggct gacaatctca atctccgagg aagatgccat gaaagccccg ctccgttcgc
     7981 tgcccctctt cgcgctagcc ctgctgatcg cctccctccc tgccgcggcg gccaaggaga
     8041 agaaggccct caccgctccc ggaacctaca tcgactggca ggacgagatc gatgacctcg
     8101 agatcatcga gtcgttcaag ctggccgact acagccgcgt cgtggtcgag cccttcgaca
     8161 cgtccaacac gccgctcccc gaggaggcgg acaacgccta ccagccggtg cggaaggtgc
     8221 tcgccgacgc gacgtctccc tttgtcacca gcctccgtga ggagctcaaa ggcaaggtcg
     8281 aggtcgccga gggggagaag ggcagtgccg cttctatagg ggctgaagag aagacgctgg
     8341 tggtgcgcgg ccgggtgacg accatggacc cgggctcccg cgccgcccgc tactgggccg
     8401 ggttcggcgc cggcgccgcc cgcaccggca tcgccgccga ggtggtcgac ggcgccaccg
     8461 gcaaggtcct cctccgcttc cagcaggagc gccgctccgg cgtcggcgcc gcgggaggca
     8521 gctacgagaa cctcctcgag cgcaacctcc gcaagatcgg cgaggacgcg gccctgatcc
     8581 tcggagcctt ctagatggcc gggttcgacg gcaaggtcgc cctcgtcacc ggcggcaact
     8641 ccgggatcgg ccgcgccacg gcgctggcgt tcgcgcgcca gggggcgcgg gtggtgatcg
     8701 ccgcgcgccg gaccgagccg ggcgaggagg tggcgcacgc gatccaccag gcgggcggcg
     8761 aggccgtgtt cgtccgcgcc gacgtcgccc gcgccgagga cgtcgaggcc ctggtgcggc
     8821 ggaccgtcga ggtctacggc ccgctcgact gcgcgctcaa caacgcggcg atcgaggggg
     8881 agcctcggac cctcgccgac tattcggagg aggagtacga cgagctgatg ggcgtcgacc
     8941 tcaagggcgt ctggctctgc atgaagcacc agatccgcca aatgctcgcc cagagtccct
     9001 ccggtggcac catcgtcaac acctcctcgg tcaacggcct gggtggcgtg gcgcatgggt
     9061 cgctctacgc ggcggccaag gccggggtgc tggcgctgac caagtcggcc gcccaggagc
     9121 tcgggggcca gggcatccgg gtcaacgcgc tggtcgccgg cggcttccgc actccgatgc
     9181 ttcagcgggt cttcgcgcgg cacggcggac cggatcccga ggcccaggcg gcggtggagg
     9241 ggcagctatc tcagctcatc cccctcggcc ggatcggcaa ccccgaggag gccgcggacg
     9301 ccgtgctctg gctctgctcg gacgcctcct cctacatgac cggcaactcg ctgatcctcg
     9361 acggcggcct cacggcttac taccgatgag gaagcccgtc atgagccggc ccccggaagg
     9421 aaccggaagc ctcgcgttgt tgtggagagc gggacctgtg atcgcggcgc cgcccgatct
     9481 ctcggcgtgc gctgcttttg gagacggaga gccgctcatg ccgacacgaa tcctggagtt
     9541 ccggcgtcca gaccagcagg gcttcacccg cgtggggcgc gtccgcacgg acgggtccat
     9601 cgccttccag ggcaaggtct accgcaccat ccgcgaggtg ccgcaggagt gcctcgccct
     9661 ccgccccgac ctcgagaccc actcgcagtg ggtccgtctc taccgcgcca tcgaccccaa
     9721 gcggccccgc cgggaggcgg accgccccga caagtagccg tcacggcacc gacaataccg
     9781 aatggcccgc atcgtcctga ccactttcgg gtccctgggg gacctccatc cgatgatcgc
     9841 gctcgccctc ggcctgcgcg agcgcggcca cgaacccgtc ttcgccaccg cggagtacta
     9901 ccggcccaag atcgaggagc tgggcttcga gctccgcgcc ctgcgacccc acatgacgcc
     9961 ggacgacccg gagctggccg ccctcgtcat ggacgccaag aaggggccgg agaacgtgct
    10021 gcggggcctc atcttccccg ccctccgcga cacgttcgag gatctccgcc gcgccgccga
    10081 gggggcggac tttctggtcg ccggcgagct gatctacggc gcgcaggtcc tcggcgagct
    10141 caccggcctc ccctgggcca acgccctgct gtcgccgatc tccttcttct ccgaatacga
    10201 cccgccggtg ccgacgccgt atccggggat ggcgaagctg ttgggcaagg cgcgctggat
    10261 cgaccgcggc atcgtccgct tcggccgctt cgtcacccgg tcctggggcc agccggtcca
    10321 cgacctgcgg cgcgagctcg gcctgccgcg cggcggccat ccgatcttcg acaagtactc
    10381 gccccacttg gtgctggccc tcttctcgcg gctgatcggc cggccccagc ccgactggcc
    10441 ggcccacacc gagcagaccg gcttcgtctt ctacgacggc agaccgggcg gcgcccgcct
    10501 gacgccggag ctggagcgct tcctggacgc cggcgagccg ccgctcgtct tcaccctcgg
    10561 ctcggcggcg gtgctcgacg ccggcgactt ctacctcgag agcgccgacg cggcgcgcca
    10621 gctcggccac cgcgccgtgc tcctcgtcgg cgccaacccg ccaccgccgg acctgccgcc
    10681 cgagatcctg gcggtggact acgccccctt ctcggacctc ttcccgcgcg ccgccgtcat
    10741 cgtccaccag ggcggcatcg gcacctgcgc ccaggccctg cgcgccggcc ggccgacgct
    10801 ggtcatgccc tacagccacg accagccgga caacgccgta cgtctggagc gcctggggac
    10861 ctcccgtacg gtgccgcggg actactattc agcgccacgg gccgcctggg agctgcggga
    10921 gctgctggcg gattcgaaat accggcagaa agccgaggag gccggaagga tcgtcagcgg
    10981 ggaggatggg ctgacggagg cgtgcgacgc catcgagaag cacctctgag ccggaatccg
    11041 ttgcagtaac ttgccatttt tggcaagtta cctccatgaa atttgcccgc tctcgaccca
    11101 gctgacgcgt agcgcgttcc ttgatcccgc gccgcttggg cggaggcttc ctccccccct
    11161 gggcgggggc gtggtaggga gcgggggagg atcgaggtgg ggggggaaac ctgaagcctg
    11221 tgttaggcag tgccgcgagg cggcgtcgac ttgaatgact tgttagccgt aaaccgcctg
    11281 agcaactaca atgctctgga agtcggactc ccggagaatg cggatctgac agccttgcgc
    11341 aatcagctcc tccgctttaa tgtgtttgga gctctttggc ttaccggcaa gcttggagat
    11401 atcctggtca ccaacgatca agagcgtggt cttctttgtg acagacgaag ccacgtcaca
    11461 tccagccctc gcggcaagat cagcagcctc acgccgtgga atagccaaag aacctgtaaa
    11521 gacggcaacg tttccataaa gcggcccttc gttgttcccg tcacgaacga ttggctgatc
    11581 acaggcggga ttgatggggc gttggacgcg ggcgaaccaa ccctcaagat ttagatcagc
    11641 gcatgcacag gctttgcaga gaacttctcc tgcggcccga gcgtcttcta gtgcatcgtg
    11701 atgcttgaac tcgatcccaa gaaactctgt gacattataa agcccatacc cctttgcacc
    11761 gaacttatct ggccatgccc gacgagcgac acgcgctgta tctagccacc gacaatcgat
    11821 ctggtcgatc ccataccttt cacaagcgag agcggtcgcc gcccgatcga atggggtgtg
    11881 gcaagcaacc accgagcccg ccataagcgc ccgcagattc tcagcgattc tcggccaggt
    11941 tggtgcgcct ctcaccttgc actcgtctat tccgtgaaga gaaacgttga agccgtcaaa
    12001 gtcgtcttct ggatcaacca ggctcgacca agagtccacg tgtgatccgt cacgaaatat
    12061 ggctacccca atttggcaga tgctggatag gtccgcattc gcggtttcaa cgtcgattgc
    12121 tgcgaactgc accgctatct cctttcacgc agttagtcgc ctcccctcct gtacgcgagt
    12181 gaatctcagg tcgcccaacg ccgactggta aaaccttgag agagccgccc aggacaagca
    12241 gtatcaaact cacgatgaac gctcctccgc caacctgata gagcctgtac gtacgccgca
    12301 caagtggctt gaaatcagaa aaccttccct tgatgatcgc gccattcgcc tgtgcgccag
    12361 caatgaagtt gaagtaaagc ccaatgccgg tggcgactag cgcaacatca gcaagcccca
    12421 agaacttagc agcagaaacc gttgaatcag ccctgcccca gatattccga caatcttctc
    12481 cgagaacgta accgaaaatg caagaactgc cgagatagta acaagaaagt gctcccccaa
    12541 gtccttcagt tccggatagt ggagcgacag gaagacctcg gagtccgact tcggcttgac
    12601 tatgcttgct atgtcttcgc cagaaagcac ctgaacttta tccttcgacc aacccgcttg
    12661 gctgagctta tgcactctgt cgcataagct ccacgcgaca ggctgtgttt ccttttattc
    12721 tcttcctgcg gtgccgctta agaaaactgt acgcccccct ttgtcagttg tcaatgcggc
    12781 agagagaggc ggaccaggaa gatgcttatc gacacatggg gtctgaggca tggggtcgcc
    12841 atcgcgtcca gatgtcctga tcgtggcttg gcctgcgaag gaagcgggct ggctgcagta
    12901 agcaccccat ggggacgcag gaggggccgg cgcttatcca cccggcctgt cgcgtttgcc
    12961 atgcggcggc ttccgcgtca ctaacctgga gagagcctgt ccggtccacg ggaagacagc
    13021 aggcgcggaa acaccgaatt tccctcttga cgcctcatcc ccacccggtc tatcctgccg
    13081 atactcgacg ttcccgcagg cactccaaga tccaaggatc gcggaacctg gtggagccgt
    13141 tcgagggaat ctggttgtag aagcgaagcg ctgcaaaggc ggtcggcaat tctgctgacg
    13201 gcccggaggt gagctggaac tcaccgccga gcctgaccgc ccacagtccg ataggaggac
    13261 tcgtgaacag cttcacgcag ggtagcactg tatccaccca ttcccccctc cccaacgcct
    13321 tcaaaccgac gtttctcggc cgtctggccg agcgggacga gccgcccacg gccggcgatg
    13381 ccgacgtggc cgggccttgg cgcatcgagc ccattccggg gcagggcttc ggcctgttcc
    13441 gggtgggcga gagcctggcc cgcggcttcg ctccgacggc ggtcttcccg tcccgatggc
    13501 tggcgctcct catggcgggc atccttcccg gcaccggcaa ggacccgctg ctcctcctct
    13561 cccagcagcc cgacgcccac ggcgtctatc ccgtcgccct cgccggtggg ggtgacgacg
    13621 gcggcgatga cggcgatggc cgcgacggcc gtggccccgg cggtcgcgtt cccgtcggtt
    13681 tccttcagct cttcgacgag cgggctgtcg atgcgttgaa cgtcgtcatc catctcctcc
    13741 aggcgccgga gtgtctggcc ttcgtgctgg aggcggcggg cggggtcacc ctcgagcgct
    13801 gcggcgccat cctcgatgag cgggtggcgg cggcggacgc cggcggggcg gctgccgggt
    13861 aggacatcct ctctctatag gcgtcaccgg tgggttcgac ctgccggtga cgcgccttcc
    13921 ggcgccctcc cgaaaagaac ccccctcctc ggtcctcccc cactccctac cacgccaccg
    13981 cccagggggg gaggaagccc ccgcacgcgc agactttggc agcagcaagc ttccatgtag
    14041 gggagggggc aagcccctcc cctacattag aagttaatgc acatgggagg caccacctcc
    14101 cgaatcacct ccccggcagt cctccaggca tccattccgg catcaactcc cggcaccgaa
    14161 ccgaaaccgc ttcccctgga tctggggatc aaggcacacg tggaaccagc ccggcgaggc
    14221 ggagggatcg atccctccgc ctcaccggct gtcctcctgt cggacggagg aatcctcagg
    14281 ggcgcggcct tggacacggc ggcgccccgt cacgattcag ttgtcaatga gccctgacgc
    14341 ttcatggccc ggcggcccca cggggcggcc ggtctgccct ggcgcgtggt ctactcgccc
    14401 ctcggggccg gccttcggcc ccggctcctg ccctgcaggc ccccgagggc cgtccggccg
    14461 cccgcgcggg catcccctcc gccctgtggg gcctgcctgt cgacccatgg ggcagcgggc
    14521 gtgccccttg gggtatccaa caggccccaa gggtccgatc ccacgccctc tgggtcatcc
    14581 tgctcgcccc gcggggcgag cggagtctgg cggatggcgc gtgaccgatt cagccggctg
    14641 aaaatgctgt tgacaggcgg cggccatcga cctagagtat acactctaga tcgattccgg
    14701 cctacgagga ccggcacgat cctgctcttt gacaatcgaa ggacacgagc cgggcgcggc
    14761 gccccgggtg gggcggcgcc gcgctcgttc gtggaagctc agaagcggta ggagaggccg
    14821 gtcagcagga tgtagtccgg gaaggtggcg tcaccctccc ggccgctgta ggggttcggc
    14881 tcccggttgc ggtagaagcc gatcggcacg ttgagcgacc aggtcgaggc gcccttcgtg
    14941 taggcgatgc cgggctcgac gaacatctcg tagcccggcc ggcgccagcc gtggctgtcg
    15001 ccgatcaggt cgtagcgcgg cagcccctcg acgcggtagg cgaggctggc gccgaacccc
    15061 gactggccga gcggtgccac ggcgcccagc cgcaccaggt actggtccgg cacggagttc
    15121 accagggtgc cctcctgggc gaagacggga ttgttcccca ggcccaggcc gacgaggatc
    15181 gagggggtgc cgttggtgtc gcgcgggttc gccaggtagg tgccggaggc gaagagcgtc
    15241 gcccggctgt actgacggaa cgcctggacg tcgaacagga tcccccagcc gccgtcgcct
    15301 ggctgcaccg actggtcgac cgccttgacc gtgtcgttgg tgccgtcgat gttcggatag
    15361 cgctgagtga cgtcgtactc gccggtcggc gccttgaccc cgagcccgag ggcgacgttg
    15421 tgccggctct ccggcgcgaa aacccagtag cggccggtca ggctgatgtc cccgagcccg
    15481 ctcgcctcct gcttggcgcg cggccccggc gatggcgaga tcggcgtcgg caccgcccag
    15541 gacgccttga tgaccgggat ccccagagca aagctgagac ggtcgctcca cgcgtaggag
    15601 atgccgaggt cgagcgcctg ctgggtgttg atgacgtagg tgcccagcgt ctcgcgctgg
    15661 cgctggcgct cgctgccgct atagtgggtg tcggagtcga ggtcccgata ggagaggctg
    15721 acctgccact cccccttctc ggggaccagc cggctggccg cgccaaacac gggcgcggtt
    15781 tggcggatga gcactcagcc ctggccggcg gcgggacgcg gagcaatcag gccggtgagg
    15841 aagacggcgg cggcggtcac ggccagcaag ggcgtgcgaa gcatcgagag gtctcctgtc
    15901 agctcttgcc atcatagctg aaagcggcta cctctccacc ctgaaccgca gcggcgcgct
    15961 ggtgacgctc ccggtgggac cggtgagctg gaccgccagc cggtactcgc ccggtggcag
    16021 ctcccccggc cggaaggtcg ccagcagccg gtccggcgcc gcgccgcctg caccactgtc
    16081 gcgctgcccc agggacagag cgctccccgg gacctccttg ccatccgccg acgtcacccg
    16141 cgcggttgcc ttcaggtcgc cgtctcccag gttccagccc accagctcga ggcgggcctc
    16201 ctgcttcgga gcgaggaccg gtctgttggc ggggatgaac ggctcgcccg ccagcatgaa
    16261 cgggtagtcc ggccgcttct ccccctcgcg ctgcgcctcg ggaaccagca cccaccggcc
    16321 cgccggctcc gggaacaggg gaggcaggag caccggcccg gcttgcgcga aggccggcac
    16381 ggtcagcggc acgacgcgca tcccgtaggc gccggtgaca ccgttccgta ctagcacccg
    16441 cagcgagtag tcgcccggcg ccaggtccac gtggccgaag aacttgagcc cggtgcgccg
    16501 cagcgtctcg ccggtcttgc cgacgtccag ccccaccgat tgcgtctgga aatcgtgcac
    16561 cgccccctgc gcgtcgagcg cgtagacgaa gatctcggcc ggcaccgcgg ccccctggtg
    16621 cccggcgagc agtcccttgc cgtccacctc gatgagcacc ggcacgtagg cctggtcccc
    16681 cgcgggctgc cggaacggcg cggcgagcac ggccaggggg atcgcgccgc cgtcctcgcc
    16741 gctcaggatg cggctcgcgg cggtcaggat cttctcgccg ccggtctgct ggctgaacgg
    16801 cttcggcgcg tagtagccgg ggcggtggac cacccgcgtg ccgcgcggcg cgtccttgag
    16861 cgtcaccttc aagcgccggt aggagccgtc gcgcggcagg tcctcgggct ggagggtgag
    16921 cacgtaggtg acgctggtgc gctggagcat ctggtccatg gcggcgccca ggtcgttgaa
    16981 gttgcggtac agctcgccgc cggtgtcgtg ggccatgagg aagaggccgt cctgcccgga
    17041 gcgccgctgc gggttcatgt cgtccccgcg caggccgccg atatcgaccg attggataac
    17101 gcagtcggcc cgccgcagct cctccagcat cttctcgagg tcgttgctcg cctcggtgtt
    17161 gccgtagcgg gcggagggat ccacccggta gaactcaccg gaggtcatgg attcgttgat
    17221 ctgatcgatc tcggcctggt cggtgatgcc ggtgatcacg gagctgtcga agccctcgga
    17281 gagatagatc acgtatttgc ggcccatgac gctgcccatc aggcgggcga ggtcggagaa
    17341 ggagcgcgag agggcggtga cctgggcctt cttctgggtg gcggcggcgc gctccgac
//
LOCUS       DASPJI010000306         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_590250, whole genome shotgun sequence.
ACCESSION   DASPJI010000306 DASPJI010000000
VERSION     DASPJI010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590250"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2152
                     /locus_tag="VEL74_08190"
     CDS             <1..>2152
                     /locus_tag="VEL74_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG62548.1"
                     /translation="RKTVGLIAPRNATEEILAGVWAELLGLDQVGMEDDFFAVGGHSL
                     LATQVVSRARELFGAEVKVRDLFEAPTVAGFAAAVEALQRDGQGLDARPVRPVPRDQD
                     LPLSFPQQRLWFLDRLASGTAASIAGTAYNLPLATRLSGPLDPGVLERALGEVVRRHE
                     VLRTTIVEKGGVPVQVIAPELRLPLPVIDLAALPDAGREAEALLLAAGQVRRPFDLAA
                     GPLVRWALLRLDAEEHVVLATAHHIVSDGWSMGILAGELGALYGAFARGEASPLPPLP
                     VQYADFAAWQRRWLTDEVLERELAYWRGQLAGAPHALELPLDRSRPAVRSGAGGTLPV
                     AIPSTLAAALVELSRREGVTLFMTLASAFQALLGRWAGQDDVLIGTPVAGRNRREIEG
                     LIGFFVNTLVLRADLAGSPPFQRLLGRARTTALDAFAHQGLPFERLVEDLGGGERDLS
                     RSPLFQVMLAFQNVPQAGAVIPGLTLSPFPFSPEVAKFELTLTLQESEAGLLGTLEYD
                     ADLFDRSTAARLLSHLGRFLDGIAADPARRIGEIPLLAAAESHQLLVEWNDTREGYAG
                     HRGLHELVAAQVERTPDAVAVSFEGEELSYAELARRSNRLAHRLRELGVGPEVPVAMG
                     VERSLAMVVGLLAVLEAGGAYVPLDPDYPAERLAYMIEDSGARLLLAQRHLAPRLPLD
                     GVHTLLLDDEAALRADGPAGRLTGGAEPGSLLYII"
BASE COUNT          254 a          743 c          817 g          338 t
ORIGIN      
        1 ccgcaaaacc gtggggctca tcgccccccg caacgccacc gaagagatcc tggcaggcgt
       61 ctgggccgag ttgctggggc tggatcaggt cgggatggag gacgacttct tcgccgtggg
      121 agggcattcg ctgctggcga ctcaggtggt ctcacgggcg cgcgagctgt tcggggcgga
      181 ggtcaaggtg cgcgacctct tcgaggcccc caccgtagcc ggcttcgcgg cggccgtgga
      241 ggctctgcag agggacggac aggggctgga cgcgcgaccg gtccggccgg tccccaggga
      301 ccaggaccta cccctctcct tcccgcaaca gaggctctgg ttcctggatc gcctcgcgtc
      361 tggcactgcc gccagcatcg ccggcacggc ctacaacctg ccgctggcga cccgcctgtc
      421 cggccctctc gatcctggcg tgctggagag ggcgttggga gaggtggtgc ggcggcacga
      481 ggtcctgcgg acgacgatcg tggagaaggg gggtgtcccc gtccaggtga tcgcgcccga
      541 gttgcggctc cccctgcccg tgatcgatct ggcggcgctg ccggatgccg gacgcgaggc
      601 cgaggcgctc ctcctggccg cggggcaggt gcgccggccc ttcgatctgg cggcggggcc
      661 cctggtccgc tgggcgttgc tgcggctcga cgccgaggag cacgtggtgc tcgccacggc
      721 gcaccacatc gtctccgacg gatggtccat gggcatcctg gccggcgagt tgggcgctct
      781 gtatggagcc tttgcccgtg gcgaagcctc tcccctgccg ccgctaccgg tccagtacgc
      841 cgacttcgcg gcctggcagc gccgctggct cacggacgag gttctggaaa gggagctggc
      901 gtactggcgc gggcaactgg ccggtgcgcc gcacgctctg gagctgccgc tggaccgttc
      961 ccggccggcg gtgcgcagcg gcgccggagg gaccctcccg gtggcgatcc cctccaccct
     1021 ggccgccgct ctggtggagc tgagccggcg cgagggggtg acgctgttca tgaccctggc
     1081 gtcggccttc caggcgttgc tgggacgctg ggcggggcag gacgacgtgc tgatcggcac
     1141 gccggtggcg ggccgcaacc ggcgcgagat cgagggactg atcggattct tcgtcaacac
     1201 gttggtgctg cgggccgatc tcgcggggtc gccgccgttc cagcgcctgc tggggcgggc
     1261 ccgcaccacc gccctggacg ccttcgcgca ccagggactg ccgttcgagc ggctcgtgga
     1321 ggacctgggt ggtggtgagc gggacctctc ccgctcaccg ctgttccagg tgatgctcgc
     1381 gtttcagaac gtgccccagg cgggagccgt gatcccgggt ctgacgctct cgcctttccc
     1441 gttcagcccg gaggtggcga agttcgagct gacgctgacc ctgcaggagt ccgaggccgg
     1501 cctgctgggc acgctcgaat acgacgccga cctgttcgac cgctcgacgg ccgcgcgcct
     1561 gctctcccat ctcggccgct tcctcgacgg gatcgccgcc gatcccgcgc ggcggatcgg
     1621 cgagatcccg ctgctggccg ccgcggagag tcaccagttg ctggtggagt ggaacgacac
     1681 ccgggagggg tacgccgggc accggggcct gcacgagttg gtggcggcgc aggtggagcg
     1741 cacgccggac gcggtggcgg tctcgttcga gggcgaggag ctgagctacg ccgagctggc
     1801 gcggcgctcc aaccgcctcg cccatcgcct gcgggagctg ggggtcgggc ccgaggtgcc
     1861 ggtggcgatg ggcgtggagc gttcgcttgc catggtcgtg ggcctgctgg cggtgctcga
     1921 ggcgggcggc gcctacgtac ccctcgatcc cgactatccg gccgagcgcc tggcctacat
     1981 gatcgaggac tcgggcgcgc ggctgctgct ggcgcagcgc catctggcgc cgcggctccc
     2041 tctcgacggc gtccacaccc tgctgctgga cgacgaggcc gccctgcggg ctgacgggcc
     2101 ggcggggcgg ctgacgggcg gcgcggagcc cggcagcctc ctctacatca tc
//
LOCUS       DASPJI010000307         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_705476, whole genome shotgun sequence.
ACCESSION   DASPJI010000307 DASPJI010000000
VERSION     DASPJI010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705476"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /gene="folP"
                     /locus_tag="VEL74_08195"
     CDS             <1..713
                     /gene="folP"
                     /locus_tag="VEL74_08195"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HYG62549.1"
                     /translation="GAESTRPGGGVYGQGARTVPAGEETGRLLPVLEALRRETDAPLS
                     ADTRKGAVARQALAAGADLINDVGALADPELGEAVAAAGCPLVLMHSRGDLATMQTGI
                     RFADVVGEVRAELAQAMDRAAAVGIPEEQLILDPGIGFGKTAAQNLELIGRLGELAAL
                     GRPLLLGASRKSFIAKAMERVGAQAPPPSERLGGSLASVAWAAATAAIVRVHDVAETA
                     QLLTVWRAVRDAAGGAEA"
     gene            710..1492
                     /gene="cdaA"
                     /locus_tag="VEL74_08200"
     CDS             710..1492
                     /gene="cdaA"
                     /locus_tag="VEL74_08200"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HYG62550.1"
                     /translation="MTLARFLELLTFRDAVDILAVAFIIYNLLLLIRGTRAVQMLLGL
                     VFVGVVYYLAQFFELPTLQTILENLLIVLPFAIIVLFQQEIRRALANFGRNPLWGLTK
                     QQKSVSAFGDIVLAATTLSSRRTGALIVIERLQGLRNYVENGIALDAVLSYDLLINVF
                     TPDTPLHDGAVIVQEDRMAAAACFLPLTPNPELSKEFGTRHRAALGITEETDAVAVIV
                     SEETGIISLAFDGDIVRDLDGKSLRNMLYKYLITDLYPQGVA"
     gene            1489..2205
                     /locus_tag="VEL74_08205"
     CDS             1489..2205
                     /locus_tag="VEL74_08205"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HYG62551.1"
                     /translation="MNERARTWGLRLLALGLAIGIWFNASVEDRLELSERLVEASVSY
                     NRPRGFVIMNQIQSVNVRLEGNRRAIRQIVPYEVNLQVDLLNQREPGTFTVNLTADMI
                     LLPEGIEVIAIEPNIIRVELEREVTRRIPVIPQLAGSPAGDAKLQEPEVFPNQVLVAG
                     PESLVSGIESLRTRPIRLDGHATTFEVNVPVDPPNPLVQVVQPTQVTVRVPLIPPQPP
                     QPTDADAAQDAADPEQEDNS"
BASE COUNT          355 a          856 c          791 g          338 t
ORIGIN      
        1 tcggcgccga gtcgacccgc ccggggggcg gggtctacgg ccagggcgcc cgcaccgtcc
       61 cggccgggga ggagaccggc cgcctgctcc ccgtgctcga agcgctacgg cgggagacgg
      121 acgcgccgct gtcggcggac acccgcaagg gcgcggtggc gcgccaggcc ctcgccgccg
      181 gcgccgatct gatcaacgac gtcggcgccc tcgccgatcc ggagctgggc gaggcggtgg
      241 ccgcggcggg ctgccccctg gtgctgatgc acagccgcgg cgacctcgcc accatgcaga
      301 ccggcatccg cttcgccgac gtggtgggcg aggtgcgcgc cgagctggcc caggccatgg
      361 accgcgccgc cgccgtcggc atcccggagg agcagctcat cctcgatccg gggatcggct
      421 tcggcaagac cgccgcgcag aacctggagc tgatcggccg cctcggcgag ctggcggcgc
      481 tgggccgccc cctgctcctg ggggccagcc gcaagagctt catcgccaag gccatggaga
      541 gagttggcgc gcaggcgccg ccgccttccg aaaggttggg gggcagcctc gcgtcggtcg
      601 cctgggccgc cgcgaccgcc gccatcgtcc gggtgcacga cgtggccgag acggcgcagc
      661 tcctcaccgt ctggcgagcc gtccgggacg cggccggagg ggcggaggca tgaccctcgc
      721 gcgcttcctc gagctgctga cgttccggga cgcggtcgac atcctggccg tcgccttcat
      781 catctacaac ctgctgctgc tgatccgcgg cacgcgggcg gtgcagatgc tcctcggcct
      841 ggtcttcgtc ggcgtcgtct actacctggc gcagttcttc gagctgccga cgctgcagac
      901 gatcctcgag aacctgctga tcgtgctgcc gttcgccatc atcgtcctgt tccagcagga
      961 gatccggcgg gcgctggcca acttcggccg taacccgctc tggggcctca ccaagcagca
     1021 gaaatcggtc tcggccttcg gcgacatcgt gctggcggcc accaccctgt cgtcgcggcg
     1081 caccggcgcg ctgatcgtca tcgagcgcct gcaggggctg cgcaactacg tggagaacgg
     1141 catcgccctc gacgcggtgc tctcctacga cctgctgatc aacgtcttca ccccggacac
     1201 gccgctgcac gacggcgcgg tcatcgtgca ggaggaccgg atggcggccg cggcctgctt
     1261 cctgccgctg acccccaacc ccgagctgtc caaggagttc ggcacccgcc accgcgccgc
     1321 actgggtatc accgaggaga ccgacgcggt ggcggtgatc gtctcggagg agacggggat
     1381 catctccctg gcgttcgacg gcgacatcgt ccgcgacctc gacggcaaga gtctgcgcaa
     1441 catgctatac aagtacctga tcaccgacct ttacccccag ggggtggcgt gaacgagcgg
     1501 gcgcgtacct gggggctccg cctcctcgcc ctcgggctcg ccatcggcat ctggttcaac
     1561 gcctcggtgg aggaccggct ggaactgtcg gagcggctgg tcgaggcgag cgtcagctac
     1621 aaccggccgc gcggcttcgt gatcatgaac cagatccaga gcgtcaacgt gcgcctggag
     1681 gggaaccgcc gggccatccg ccagatcgtc ccgtatgagg tgaacctgca ggtcgacctg
     1741 ctgaaccagc gcgagccggg gaccttcacc gtcaacctga ccgccgacat gatcctgctg
     1801 ccggagggca tcgaggtgat cgccatcgag cccaatatca tccgggtcga gctggagcgc
     1861 gaggtcacgc ggcgcatccc ggtgatcccg caactcgccg gcagtccggc cggggacgcc
     1921 aagctccagg agccggaggt gttcccgaac caggtcctgg tagccggacc ggagtccctg
     1981 gtcagcggca tcgagtcgct ccgcacccgt ccgatccggc tggacggcca cgccaccacc
     2041 ttcgaggtca acgtgccggt ggacccgccc aaccccctgg tccaggtggt gcagccgacg
     2101 caggtcacgg tacgggtgcc gctgatacca ccccagcccc cccaacccac cgacgcggac
     2161 gcggcacaag acgcggccga tccggaacaa gaggacaatt cttgagcaag aagctctttg
     2221 gaaccgacgg catccgcggc cacttcggtg agccccccct cgaccacgac accgtgaccg
     2281 cgctggcgct gcatctggcc gcgaccctcc gggagctcgg caatcccctg gtggtcctcg
//
LOCUS       DASPJI010000308         4344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_650392, whole genome shotgun sequence.
ACCESSION   DASPJI010000308 DASPJI010000000
VERSION     DASPJI010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650392"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4328)
                     /locus_tag="VEL74_08210"
     CDS             complement(<1..4328)
                     /locus_tag="VEL74_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62552.1"
                     /translation="MVRTGAGGDRRLVGYVGLYPDSAASAGEVRAALQEILPDYMVPS
                     AVVVLEALPRTSNGKVDRKALPEPEAGGEASDIFVAPRTMVEEILAGIFAEVLGAARV
                     GARDDFFELGGHSLLAARLASRLRDALGVELPLRQLFESPTVEELAVVVEALRHSDEP
                     AAPPLRPLESREGLLPLSYAQERLWLLEQLEPGSATYNIPAAVDLEGHLDVAALRQGL
                     EALVARHEPLRTVFTAIAGRPSQCIVEPPAVPLPEVDLSALRDPEAEARRLGAQEASR
                     PFDLERGPLLRAWLLRLERQRRRLLLTLHHIVSDGWSTGVLVRELGALYGRGMQVRLP
                     ELPVQYADYAAWQRSWLEGETLEREAGFWRQRLAGAPPMLELPTDRPRPAVRSGRGGL
                     RHLELAPALVDGVMRLARRQGATPFMVLLAGFYALLHRVSGVEDLVVGSPVAGRDRSE
                     TEGLIGFFVNTLVLRAAVSGDQTAGELVRRARETVLAAQSHQWLPFEKLVELLQPVRD
                     LSHTPVFQVMLAYQNDPLPPLELPELRLRVLEAESRAARFDLTVALRPVAGALAGVIE
                     YAADLFDATTIDRLAGSFAALLETLAAEPESQIAALPVLTAAQRHQLLIEWNSTGLTV
                     SGLSVHDQLALQAARDPGALAVAGGGKALTYGELNARANRLARRLRRLGVGPEVLVGV
                     CLERSPELVVAVLGILQAGGAYVPLDPGYPAERLSYMVADAGTPVLISREGLRSSLPT
                     GVAEVLCIEEVEAMDEEPARGRELPRSSSGHLAYVIYTSGSTGRPKGVAVAHPALSNL
                     VAWHLDTYRVTPADRYTQIASPAFDASVWEIWPPLCAGASLHIPDEEARLSPARLVHW
                     LADTGVTAAFLPTPLAESALAEPWPETSALRLLLTGGDRLRRGGQESGFRLLNHYGPT
                     EAAVVSTWGPVARQAELVSPPPIGRPVSNAAAYLLDAWGGPVPVGSAGEILVGGAGLA
                     RGYVGRPDLTAERFVPHPFAAEPGARLYRTGDLGRYLPGGAIEFLGRVDRQVKVRGFR
                     IELGEVEAALARHPAVRDTVVAAAEGGGGLVAYVVAAPGSGLTAAELRAFAGHALPDV
                     MVPRAVVLLDRLPLTPNGKVDHRALPAAGLEEVAEVYVAPRTPVEEIVAEVWAEVLGA
                     PRVGVRDDFFSLGGHSLLAAQVVSRVREALGVELTLRELFEGPTVEAWAQRVEEARRR
                     ESGAATPPLVPVPREAELLPLSFTQERLWFLDQLEPGAAAYNIPVAVELAGDLDAAAL
                     AASLVGLVRRHEALRTVFGEAAGRPFQTVREAASMAMPLVDLQALPPAAGETEARRLT
                     GDEAARPFDLTRGPLLRAGLLRLAVERHRLLLTLHHIVSDGWSMGVLVRETAALYSAL
                     RQGEAPSLPPLPVQYGDYAVWQRSWLRDDLLEREVAHWRQRLAGVPDALDLPTDRPRP
                     AVRGRRG"
BASE COUNT          668 a         1677 c         1503 g          496 t
ORIGIN      
        1 ccacgccggc cgcgcaccgc cggacgcggc cggtccgtag gtaggtcgag agcgtcaggc
       61 acccccgcca gccgctggcg ccagtgggcc acctcccgct ccaggaggtc gtcgcgcagc
      121 cagctccgct gccacacggc gtagtcgccg tactgcaccg gcaggggcgg cagggacggg
      181 gcctcgcctt gccgtagcgc gctgtacagg gccgccgtct cccgcaccag gactcccatg
      241 gaccagccgt cggagacgat gtgatggagg gtgagcagca ggcggtgccg ctccacggcg
      301 agccggagca ggccagcgcg cagcaggggg ccgcgggtga gatcgaatgg acgcgccgcc
      361 tcgtccccgg tgaggcgccg cgcctcggtc tctcctgcgg ccggcggcag agcctggagg
      421 tctaccagcg gcatcgccat cgaggccgcc tcccggacgg tctggaacgg ccggccggcc
      481 gcctccccga agacggtacg cagcgcctcg tggcggcgca ccaggccgac gaggctcgcg
      541 gcgagggccg ccgcgtcgag atcccccgcg agctccaccg ccacggggat gttgtaggcg
      601 gcggccccgg gctcgagctg atcgaggaac cagagccgct cctgggtgaa agacaagggg
      661 agaagctcgg cctccctggg caccgggacc aggggcgggg tggccgcgcc gctctcgcgc
      721 cgccgcgcct cctccacccg ctgggcccaa gcctcaaccg tggggccttc gaacagctcg
      781 cgtagcgtga gctccacccc cagggcctca cggaccctcg acaccacctg cgccgccagc
      841 agcgagtggc cgccgaggga gaagaagtca tcgcggaccc cgacccgcgg cgcacccagc
      901 acctcggccc atacctccgc cacgatctcc tccacaggcg tgcgcggcgc gacgtagacc
      961 tccgccacct cctccagacc ggcagccggc agggcgcgat ggtccacctt gccgttcgga
     1021 gtcaaaggga ggcggtccag caggaccacg gcccgcggca ccatgacgtc gggcagggcg
     1081 tggccggcga aagcgcgcag ctcggcggcg gtcagcccac taccaggcgc ggcgacgacg
     1141 taggccacga ggccaccgcc cccttcggcg gccgccacca ccgtgtcccg taccgcgggg
     1201 tgccgcgcca gcgccgcctc cacctcgccg agctcgatcc ggaagccgcg caccttcacc
     1261 tgccggtcga cacggccgag gaactcgatg gcaccgccgg gcaggtagcg cccgaggtca
     1321 ccggtgcgat agagccgcgc tcccggctcg gcggcgaacg gatgaggtac gaacctctcc
     1381 gccgtcaggt ccgggcggcc cacgtagccc cgcgccaatc cggcgccgcc caccagaatc
     1441 tcgcccgccg accccaccgg caccggtccg ccccaggcgt ccagcaggta ggccgcggca
     1501 ttggagacag gacggccgat ggggggcggc gataccagct cggcctgccg cgccaccggt
     1561 ccccaggtag agaccaccgc ggcctcggtg ggtccgtagt ggttgagcag ccggaagcca
     1621 gactcctgtc ccccgcgccg cagccggtcg ccgccggtga gcagcaggcg cagggccgag
     1681 gtctccggcc agggctcggc cagggcggat tcggcgagtg gcgtggggag gaaagccgcg
     1741 gtcactccgg tgtcggccag ccaatggacg agccgcgcgg gagagagccg cgcctcctcg
     1801 tcgggtatgt gcaggctcgc cccggcgcac agcggcggcc agatctccca gaccgaggcg
     1861 tcgaacgccg gcgaggcgat ctgggtgtag cggtcggcgg gcgtgacacg gtaggtgtcc
     1921 aggtgccacg cgaccaggtt cgacagggcg ggatgcgcga cggccacgcc cttgggccgg
     1981 ccggtggagc cggaggtgta gatcacatag gccaggtgac cggaggagga acgaggcagc
     2041 tctcggcccc gcgccggctc ctcgtccatt gcctccacct cttcgatgca cagaacctcg
     2101 gcgacgccgg tgggcaggga ggaccgcaac ccttcgcggc tgatcagcac cggggtcccc
     2161 gcgtccgcca ccatgtagga cagccgctcg gcgggatagc ctggatcgag gggcacgtac
     2221 gcgccccccg cctggaggat gccgagcacc gccaccacca gctccggcga gcgctccagg
     2281 cagaccccca ccaatacctc cgggccgacg cccaggcggc gcagacggcg ggcgaggcgg
     2341 ttcgcccgcg cgttcagctc gccatacgtc agggccttgc cgcctccggc tacggccaac
     2401 gcgccggggt cacgcgccgc ctggagagcg agttgatcgt ggacactgag accggacacc
     2461 gtcagaccgg tggagttcca ctcgatgagg agctgatggc gttgcgccgc ggtgagcacg
     2521 gggagggcgg cgatctggct ctcaggctcc gccgccagcg tctcgagcag agccgcgaag
     2581 gaaccggcca agcggtcgat ggtggtggca tcgaagaggt ccgcggcgta ctcgatgacg
     2641 ccggcgagcg ctccggcgac cggacggagg gcgacggtga ggtcgaagcg cgcggcccgg
     2701 ctctccgcct cgaggacgcg cagccgcagc tccggcagct cgagaggggg tagcggatcg
     2761 ttctggtagg ccagcatcac ctggaaaacc ggcgtgtgac tgaggtcgcg caccggctgc
     2821 agcagctcca ccagcttctc gaagggcagc cactggtggg actgcgccgc cagcaccgtc
     2881 tcccgcgcac gccgcaccag ctcgccggcc gtctggtctc cggacaccgc cgcacgcagc
     2941 accaacgtgt tgacgaagaa cccgatcaga ccctcggtct ccgagcggtc ccggcccgcg
     3001 acaggcgagc ccaccaccag gtcctccacc ccactgaccc gatgcagcag cgcgtagaag
     3061 ccggccagga gcaccatgaa cggcgttgcc ccctggcgcc gcgccagacg catcactccg
     3121 tccaccagcg ccggcgccag ctccaggtgg cgcaggccgc cccggccgct gcgtaccgcc
     3181 ggccgcggcc ggtcggtggg caactccagc atcggcgggg cgccggccag ccgctggcgc
     3241 cagaagcccg cctcgcgctc cagcgtctcc ccttccagcc agctccgctg ccaagctgcg
     3301 tagtccgcgt actgcaccgg cagctccggc agtctcacct gcattcctct gccgtagagg
     3361 gcccccagct cgcgcaccag cacaccggtg gaccagccgt cggagacgat gtggtgcagg
     3421 gtcagcagca ggcgacggcg ctgccgttcc aggcgcagca gccacgcccg cagcagcggg
     3481 ccccgctcca ggtcgaacgg acgcgacgcc tcctgcgctc ccaggcgccg ggcctcggct
     3541 tccggatctc gcagggccga caggtccacc tcgggcagcg gcaccgccgg cggctccacg
     3601 atgcactggg agggacggcc cgcgatggcc gtgaagaccg tgcgcagggg ctcgtgccga
     3661 gcaaccagcg cctccaggcc ctgacgcagt gccgccacgt ccagatgccc ttcgagatcc
     3721 accgccgccg ggatgttgta ggtggccgat cccggctcga gctgctccag cagccagagc
     3781 cgctcctgcg cataggacag cggcagcagg ccctcccggc tctccaatgg acgcagcggc
     3841 ggcgccgccg gctcgtcgct atgccgtagc gcctcgacca ccaccgccag ttcctccacc
     3901 gtcgggctct cgaacagctg ccgtaacggc agctcgactc ccagggcatc gcgcagccgc
     3961 gaagccagcc gcgccgccag caacgagtgg ccgcccagct cgaagaagtc gtcgcgggcg
     4021 ccgacccgcg ccgcgcccag cacctcggcg aagatccccg ccaggatctc ctccaccatc
     4081 gtgcgcggag ccacgaagat atccgacgcc tctcccccgg cctccggctc gggcagcgcc
     4141 ttgcggtcca ccttgccgtt ggacgtccgc ggcaaggcct ccagcaccac caccgccgac
     4201 ggcaccatgt agtcggggag gatctcttgc aacgcggccc ggacctcgcc ggccgaggcc
     4261 gcgctatccg ggtacaggcc cacgtagccc accaggcgcc ggtcgcctcc ggctccggtc
     4321 cgcaccacca cagccgcctc ccgc
//
LOCUS       DASPJI010000309         5965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_419165, whole genome shotgun sequence.
ACCESSION   DASPJI010000309 DASPJI010000000
VERSION     DASPJI010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419165"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="VEL74_08215"
     CDS             complement(<1..1197)
                     /locus_tag="VEL74_08215"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HYG62553.1"
                     /translation="MSSTPSPRPILEVARDLGLDPRHVIPYGEDKAKIRPAARESGRP
                     PGKLVLVSALTPTGAGEGKTTMSIGLAQGLAKLGQRACLALREPSLGPTFGMKGGATG
                     GGRSVLVPSEDINLHFTGDFHAITSAHNLLAAMLDNHIHQGNALGIDARRVQWRRVLD
                     LNDRALRRIVTGLGGPMEGVPREAGFDITAASEVMAALCLAEDDSDLRQRLGRLLVAL
                     TGDGRPVTAADIHAVGAMMVLLKDALMPNLVQTGEGVPAVVHGGPFGNIAHGCNSVIA
                     TKTALAYADWVVTEAGFAFDLGAEKFFDIKCQSAGLDTAAVMVVATIRALKRHGGVAK
                     DGLATPDPAAVERGLGNLEKHVESVRIFGETPIVGLNRFHTDSPEEIEVVRQACERLG
                     TPFAVAD"
     gene            complement(1341..3470)
                     /locus_tag="VEL74_08220"
     CDS             complement(1341..3470)
                     /locus_tag="VEL74_08220"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HYG62554.1"
                     /translation="MNRRRVSSLAALLLLASALVGSAQKAAPPAPQPSKEIQFPAFEQ
                     RTLGNGLRVVVIEHRETPSVSAQIILPAGRLYSTNAKAGLASATASLLTQGAGKRSAQ
                     EIAAAIDLVGGTLNAGAGSDAGEASVRVTSDQLDLGLGLLADIVLRPTFPEEEIERWR
                     RQALSGLQIQQNDPGYLAGAALNRAVFGDHPYGQPSDGTPETVRGLTREDMVAFHRAH
                     YVPNGAILAIVGNVRPADAFARAERFFGGWTKGEEPKAPAFQAAKPQSPRILVVDKPD
                     AVQTQILVGQTGIPYNDPQLYLAEVYSAVVGGGSNARLYEEVRTKRALSYGAYSDFDP
                     RRQTGIFLAGTSTKTESTVDALEQVQTVLRGMGQQPVPETELTGRKGFITGAFPLEIE
                     TPEGIAGKVVEAMFFGLGKDFIESYNDRIEAVTAAELQRFAANAMNPSHSTIVLVGNA
                     SAFASDLEKRFGKFETIPVAELDLLRADLRRARDTAATAPVSDADRTRGLELLRLARQ
                     ALGGQAFAEQRSQVSKGSGSVTPPGMPQAMPIQSVTIYEVFPAKSRTEMTLPFGQMIQ
                     GFDGQTGWVSVMNQVQEQPQMGQEQYFGLNVLRRFDRPGYTVRPLADAEVQGKKAQVV
                     EVSDDAGHTTRFFVDPQSHLLLKSSFEAGGQTTETVYSDYREVGGLQIAHGMTVIQNG
                     SPMAEMKLTEVQVNPAIDDALFKKPQS"
     gene            complement(3467..4921)
                     /locus_tag="VEL74_08225"
     CDS             complement(3467..4921)
                     /locus_tag="VEL74_08225"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYG62555.1"
                     /translation="MRIRTTLLAALALVAPAQGLAQGSAPATTPGTAQGAGAPRALQV
                     TPPKLEVSRHELPNGLKVLLHEDHSVPVVNLQILYHVGSKNEAPGRSGFAHLFEHIMF
                     KGSENVAPEEHKVYVENIGGVYNAGTSFDTTVYWETIPSNYLERFLWLEADRMRSLNI
                     NQANLESERGVVKEEKQLRYETPPFGRLLEHMLANTYKVHPYRLPAIGKVEDLNAATL
                     DDVRAFHSTYYVPNNATMVISGDFEPRQALQWVEKYFGSIPKGKPISRDLPKEPAQTA
                     ERRAMVYDNNTPLPLVMMTYKIPEAGHPDTYALEVATNILSSGQSSRIFRDLVYEKQA
                     AVAAGGQALVLEDPGVFFFYAILQAGHTAEEGEKALLDHAQRLQNEPVSREELDKAKN
                     QIVSGLVFSRQTVQQKADAIGYAAVVMGDVDMVNQQLPLYQKVTAQDVQRVARKYFSP
                     ENRTVIYMLPESSRPAEGAAPAPGAQSDKSEVKP"
     gene            complement(5031..>5965)
                     /locus_tag="VEL74_08230"
     CDS             complement(5031..>5965)
                     /locus_tag="VEL74_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYG62556.1"
                     /translation="EGVVASIEVSNRNWGVVRVGRSSTWHADDSPGVPTLMMAAEPYN
                     HVVRLAERGEGVELEVDLQARFLDGDPMAHNTLAELPGTDKKGEIVMAGAHLDSWHGA
                     TGATDNAAGVAVAMEAVRILKALGVKPRRTIRVALWAGEEQGLLGSSHYVQEHVAARA
                     TPDDPAQKKVSPSLWKGPLTLKPEHAKLSAYFNLDNGGGKLRGIFAEENAAAAPIFAA
                     WLAPLHDLGADTVTLRKTGQTDHVPFAGAGVPGFQFIQDELDYSARTHHSNMDTVDHL
                     EKRDLMQASVVLATFLYHAATRPEMLPRVPVPGL"
BASE COUNT          854 a         1951 c         2163 g          997 t
ORIGIN      
        1 gtccgccacg gcgaaaggag tccccaggcg ctcgcaggcc tggcgcacca cctcgatctc
       61 ctcgggagag tcggtatgga agcggttgag gcccacgatc ggggtctcgc cgaagatgcg
      121 gacgctctcc acgtgcttct ccaggttgcc gagcccccgc tcgacggcgg ccgggtccgg
      181 tgtcgcgagg ccgtccttgg ccacgccgcc gtggcgcttc aaggcgcgga tcgtggccac
      241 caccatcacg gcggcggtgt cgagcccggc cgactggcac ttgatgtcga agaacttctc
      301 cgcccccagg tcgaaggcga agccggcctc ggtcaccacc cagtcggcat aggccagggc
      361 ggtcttggtg gcgatcaccg agttgcagcc atgagcgatg ttgccgaacg gaccgccgtg
      421 gaccaccgcc ggcactccct cgccggtctg caccagattc ggcatcaggg cgtccttgag
      481 cagcaccatc atggcgccga ccgcgtggat gtctgccgcg gtcaccggcc tgccgtcccc
      541 ggtgagagcg acgagcaggc ggccgaggcg ctgccgcagg tcgctgtcgt cctccgccag
      601 gcagagggcg gccatcacct cggaggccgc ggtgatgtcg aagccggcct cccgtggcac
      661 cccttccatc gggcccccca ggccggtcac gatgcggcgg agcgcccggt cgttgaggtc
      721 caggacccgg cgccactgca cccggcgcgc gtcgatcccc agggcgttcc cctggtggat
      781 gtggttgtcc agcatggccg ccaggaggtt gtgggcgctg gtgatggcgt ggaagtcgcc
      841 ggtgaaatgg agattgatgt cctccgacgg taccagcacc gaccgtccgc cgccggtggc
      901 tccccccttc atgccgaagg tggggccgag cgagggctcg cgcagggcga ggcaggcgcg
      961 ctgccccagc ttcgccagcc cctgggcgag gccgatcgac atggtggtct tgccttcacc
     1021 ggcccccgtg ggggtgaggg cggagaccag caccagcttg ccgggaggac gcccggattc
     1081 ccgggcggcg ggacggatct tggccttgtc ttcgccgtac ggaatgacgt ggcgcggatc
     1141 gaggccgagg tcgcgggcaa cttcgaggat cgggcgaggc gatggcgtcg agctcatgaa
     1201 tctcctcaag aaagttggca acgaaggggg ggacggtccc gggggaagat atcgcgagaa
     1261 ggtgggctgc cgctagaaac gccagggccg ctcttgcgag cggccctggc ggggaaaggc
     1321 aggaagggag agaagagggg tcagctctgc ggcttcttga agagggcgtc gtcaatggcc
     1381 gggttgacct gcacctcggt cagcttcatc tcggccatcg gcgagccgtt ctggatgacg
     1441 gtcatgccgt gggcgatctg aagcccgccg acctcccggt aatccgagta gaccgtctcg
     1501 gtggtctggc caccggcctc gaacgaggac ttgagcagca gatggctctg cgggtcgacg
     1561 aagaagcggg tggtgtgacc cgcgtcgtcg gagacctcga cgacctgcgc cttcttgccc
     1621 tggacctcgg cgtcggcgag cggccgcacc gtgtagcccg gccggtcgaa gcgccgcagg
     1681 acgttgaggc cgaagtactg ttcctggccc atctggggct gctcctgcac ctggttcatg
     1741 acgctgaccc agccggtctg gccgtcgaag ccctggatca tctggccgaa gggcagcgtc
     1801 atctcggtac gggacttggc cgggaagacc tcatagatgg tgaccgactg aatgggcatc
     1861 gcctgcggca tcccgggcgg ggtgacgctg ccagacccct tggagacctg cgagcgctgc
     1921 tcggcaaagg cttggccgcc cagcgcctgc cgcgccaggc ggaggagctc caggccccgg
     1981 gtacgatcgg cgtccgagac cggggccgtg gcggcggtgt cccgggcgcg ccgcaggtcg
     2041 gcgcgcagca ggtcgagctc cgccacgggg atcgtctcga acttgccgaa ccgcttctcc
     2101 aggtccgagg cgaaggccga ggcgttgccc accagcacga tggtggagtg ggagggattc
     2161 atggcgttgg cggcgaagcg ctggagctcg gccgcggtca ccgcttcgat ccggtcgttg
     2221 tagctctcga tgaagtcttt cccgaggccg aagaacatcg cctcgaccac cttgccggcg
     2281 atcccctccg gggtctcgat ctccaacggg aaggcaccgg tgatgaaccc cttcctgccg
     2341 gtgagctccg tctcgggcac cggttgctgg cccatgccac gaagcaccgt ctgcacctgc
     2401 tccagggcgt cgaccgtcga ctcggttttg gtcgaggtgc cggcgaggaa gataccggtc
     2461 tggcgccggg gatcgaagtc gctgtaggca ccgtaggaga gggcgcgctt ggtgcggacc
     2521 tcttcataga gccgggcgtt ggagccgccg cccaccaccg cgctgtagac ctcggcgagg
     2581 tagagctgcg gatcgttgta ggggataccg gtctgcccga ccaggatctg ggtctggacc
     2641 gcgtccggct tgtccacgac caggatcctg ggcgactggg gcttcgccgc ctggaaggcg
     2701 ggggccttcg gctcctcgcc cttggtccaa ccgccgaaga agcgctcggc gcgggcgaag
     2761 gcgtcggccg gtctgacgtt gccgacgatg gcgaggatcg ccccgttggg cacatagtgg
     2821 gcccggtgga aggcgaccat gtcctcccgg gtcaggccgc gcaccgtctc gggggtgcca
     2881 tccgacggct ggccgtacgg atggtcgccg aagacggccc ggttcagggc ggcgccggcg
     2941 agataccccg ggtcgttctg ctggatctgc aacccgctca gcgcctgccg gcgccagcgc
     3001 tcgatctcct cctcggggaa ggtcggccgc aggacgatgt cggcgagcag cccgaggccg
     3061 aggtcgagct ggtccgaggt gacccggacg gaggcttcac cggcatccga gccggccccg
     3121 gcgttgagcg tgccgcccac gaggtcgatg gcggcggcga tctcctgggc cgaccgcttc
     3181 cccgccccct gggtgaggag gctggcggtg gcggaggcca gacccgcctt ggcattggtg
     3241 gagtagagac ggcccgccgg caggatgatc tgggcgctca ccgagggggt ctcccggtgc
     3301 tcgatgacca cgacccgcag gccgttgccc agagtcctct gctcgaaggc cgggaactgg
     3361 atctccttcg agggctgcgg cgcgggcggc gccgccttct gtgccgaccc aaccagcgcg
     3421 ctagccagca ggagcagggc ggcaagagag ctgacccgtc gtctgttcat ggcttcacct
     3481 cgctcttgtc gctctgggca cccggtgccg gagcggcacc ctcggcgggg cgggaggact
     3541 ccgggagcat gtagatcacg gtgcggttct cggggctgaa gtacttccgg gccacccgct
     3601 gcacgtcctg ggcggtgacc ttctggtaga gggggagctg ctggttgacc atgtcgacgt
     3661 cgcccatgac caccgcggcg tagccgatgg cgtcggcctt ctgctgcacc gtctggcggc
     3721 tgaacaccag gccggagacg atctggttct tggccttgtc cagctcctcg cggctcaccg
     3781 gctcgttctg gagccgctgg gcgtggtcga gcagcgcctt ctcgccctcc tcggcggtat
     3841 ggcccgcctg caggatggcg tagaagaaga agactcccgg atcctcgagc accagcgcct
     3901 ggccgcccgc ggctacggcc gcctgcttct cgtacaccag gtcgcgaaag atgcgggagc
     3961 tctggcccga ggagaggatg ttggtcgcca cctcgagggc ataggtatcg gggtgccccg
     4021 cctcggggat cttgtaggtc atcatgacca gggggagggg cgtgttgttg tcgtagacca
     4081 tcgcgcgccg ctcggcggtc tgcgccggct ccttggggag gtcgcgcgag atcggcttgc
     4141 ccttggggat ggagccgaag tacttctcga cccactgcag ggcctgcctg ggttcgaagt
     4201 cgccggagat gaccatcgtg gcgttgttgg gcacgtaata ggtgctgtgg aacgcccgca
     4261 cgtcatcgag cgtagcggcg ttcagatcct ccaccttgcc gatcgccggc agccggtagg
     4321 gatggacctt gtaggtgttc gccagcatgt gctcgaggag ccggccgaag gggggcgtct
     4381 cgtaacggag ctgcttctcc tccttgacca cgccgcgctc ggactcgagg ttcgcctggt
     4441 tgatgttgag ggaccgcatg cggtcggcct ccagccagag gaaccgctcc aggtagttgc
     4501 tcgggatggt ctcccagtag accgtggtgt cgaagctggt gccggcgttg tagacgccgc
     4561 cgatgttctc gacgtagacc ttgtgctcct ccggcgccac gttctcggag cccttgaaca
     4621 tgatgtgctc gaacaggtgg gcgaagccgc tccgccctgg agcctcgttc ttggagccca
     4681 cgtggtagag gatctggagg ttgacgaccg ggaccgagtg gtcttcgtgg agcaggacct
     4741 tgaggccgtt cggcagctcg tgccggctca cctccagctt gggaggcgtc acctggagcg
     4801 cgcgcggagc ccccgcccct tgggccgtcc ccggggtggt cgccggggcg gagccctggg
     4861 ccaggccctg cgccggcgcg accagagcca gcgccgccag cagtgtggtt cttatcctca
     4921 ttccatctcc ttgggttgtg gtcggagccg tcgccgcggc gaggcggaga cacgaaagcc
     4981 agggggcggc cccggctccc gtcctttata cgctgttctg gcgaagaagt ttagagtcct
     5041 ggtacgggaa ccctcggcag catctccggc cgcgtcgccg catgataaag gaaggtggcg
     5101 agcaccaccg aagcctgcat caggtcccgc ttctcgagat ggtccacggt gtccatgttc
     5161 gagtggtggg tgcgggcgct gtagtccagc tcgtcctgga tgaactggaa cccggggacg
     5221 ccggcgccgg cgaaggggac gtgatctgtc tgcccggtct tgcggagcgt cacggtgtcg
     5281 gccccgaggt cgtggagcgg ggcgagccag gcggcgaaga tgggtgccgc agccgcgttc
     5341 tcctcggcga agatgccgcg cagcttcccc ccgccgttgt ccaggttgaa ataggccgag
     5401 agcttggcgt gctcgggctt cagggtcagc gggcccttcc acagcgacgg ggagaccttc
     5461 ttctgggccg ggtcgtcggg ggtggcccgg gcggcgacat gctcctggac gtagtggctg
     5521 gagcccagaa gcccctgctc ctcgccggcc cacagggcga cgcggatggt ccgccggggc
     5581 ttgaccccca gagccttgag gatgcgcacc gcctccatgg ccacggcgac ccccgcggcg
     5641 ttgtcggtgg cgccggtggc gccgtgccag gagtcgagat gggccccggc catgacgatc
     5701 tcgcccttct tgtccgtccc cgggagctcg gccagggtgt tgtgggccat gggatcgccg
     5761 tcgaggaagc gggcctggag gtcgacctcc agctccaccc cctcgccgcg ctcggcgagc
     5821 cggaccacgt ggttgtacgg ctcggcagcc atcatcaggg tgggaacccc ggggctgtcg
     5881 tcggcgtgcc aggtggagct ccggcccacc cggacgaccc cccagttccg gttgctcacc
     5941 tcgatcgagg ccaccacccc ctcgg
//
LOCUS       DASPJI010000310         5712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_590436, whole genome shotgun sequence.
ACCESSION   DASPJI010000310 DASPJI010000000
VERSION     DASPJI010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590436"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VEL74_08235"
     CDS             <1..455
                     /locus_tag="VEL74_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HYG62557.1"
                     /translation="VAQAFHWFDFDRFFAEAHRVLAPGGALALWTYNLARVSPEVDAV
                     VDRLARQIAGPYWPPERRWVDEEYRTIPFPFPEVEIPPFEHVEHWDLERLLLYIGTWS
                     ACQRYLAATGQDPIALIRGDIEAAWGPVDRPRRIQWPIFLRASQPIEP"
     gene            496..1272
                     /locus_tag="VEL74_08240"
     CDS             496..1272
                     /locus_tag="VEL74_08240"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HYG62558.1"
                     /translation="MITALVSRKGGVGKTTTAVNLAAALAAQGRRVLLVDLDSQASAS
                     LSLGVRREDLPPSSADLLLSGTPAREVIRRTSVEGLDLITASSDLSRADLDLAPFRSR
                     EIRLRSSLSAVAGDYGFVLLDCPPSMSLVQINALVAAQTFIVPVIPQFLAAAGVRNLI
                     ESAERLSWNGSRPARPLGVLLTLVDYRSKATRQTVDRLRAEYGSLVFAMEIRVNTRLA
                     EAPAAGQTIFQYDAGATGAEAYRLLADEYVLRAGAAERKG"
     gene            complement(1380..2885)
                     /locus_tag="VEL74_08245"
     CDS             complement(1380..2885)
                     /locus_tag="VEL74_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYG62559.1"
                     /translation="MPVSPSAMKRSLVSLAVATVAGIAGIAGISAMALQSAPSSPPPA
                     ENAGPAPSPPPSLTAEIRARAATLRDEALRGTRAYEIARSLVVEVGPRPAGSKADAAA
                     VEWALRKMKELGFANVRAEKVTVPHWVRGQESGRILAPYPQPVVLAALGGSVGTTDEG
                     IEAEVIEAASVEALDALDPARVRGKIVFLNVRMERTRDEKGYGDAVPARSAGPSHAAR
                     LGAVAVLIRSIGTDRDRVPHTGGTRYEEGVPRIPAAALSNPDADLLAAQVASGKPVTF
                     RLKLGSRLLGEAESANVIGEVRGRERPEEIVLIACHLDSWDLGTGALDDAAGCGIVAE
                     AARRIAPLRPRRTIRVVFFANEEFGLSGARAYAEAHAGELAKHVLAAESDFGAGRVWR
                     LRSRVAPAALPFVGQLAELLRPLGVEQGDNNESSGGADLIPLQPAHVPVLAFGQDATH
                     YFDWHHTANDTLDKIDPKELDQNVAAWAAVIYAAAETPGTFGPAPDYVPEP"
     gene            complement(2985..4142)
                     /locus_tag="VEL74_08250"
     CDS             complement(2985..4142)
                     /locus_tag="VEL74_08250"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HYG62560.1"
                     /translation="MAEEKLRLNWINTIFLLGTLAVALVGTPWYIVKHGLGWPETVTF
                     LVLWTCVGLSVTAGYHRLFSHKTFQASWPVRLFFLVFGAAALENSVLNWAADHRIHHS
                     HVDHDRDPYNIQRGFWWAHIGWIFYDVPRDNSAAVRDLHEDPLVVWQHRWYKLIGIGV
                     ALGIPLIVGLATGRVLGCLLIGGVARIVASHHTTFFINSACHMLGRQPYSREHSARDS
                     GLMAILAFGEGYHNYHHSFPFDYRNGIKTWHFDPAKWIIFLLEKVGLARDLRRASEAA
                     VLKAKVEVQFEQAQETMIQAGSDLRERYEQRVHDAYARVQAALHELVALQRRHKQPAA
                     ASAAIGAEGTGEPHLSLEERVRQTYHAVEQALHEWKQVLQQVHQMPASAAA"
     gene            complement(4446..4898)
                     /locus_tag="VEL74_08255"
     CDS             complement(4446..4898)
                     /locus_tag="VEL74_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HYG62561.1"
                     /translation="MSNGMIRTCPSCGKKNRVLYARLGAEARCGSCQAVLPPPAEPLA
                     VDQGGELSALLRESSLPVLVDFWAPWCGPCRAVAPELQKVAAKNAGRFLVVKVNTDVD
                     PSVGATHRVSSIPTMAVFQGGREVARTSGARPAAAIEGFVQQSLGVAV"
     gene            5008..>5712
                     /locus_tag="VEL74_08260"
     CDS             5008..>5712
                     /locus_tag="VEL74_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HYG62562.1"
                     /translation="MEGYRVKPGTKVELNGIDAGSTDEAKGGKSKSAEAFAELNQKLE
                     TLQEMLWAQGRHRVLVVLQGMDTSGKDGVIRHVFDRVNPTGVKVTSFKAPTDLELAHD
                     FLWRIHSQVPADGELVIFNRSHYEDVLVARVRNLVPPDVWRRRYDQINDFERLLAETG
                     TTILKFFLHIDKDEQKERLQARLDDPTKHWKFRRGDLDDRRLWDEYQRAYEEALSRTS
                     TDHAPWYIVPANRKWYR"
BASE COUNT          851 a         2032 c         2039 g          790 t
ORIGIN      
        1 cggtggccca ggccttccac tggttcgact tcgaccgctt cttcgccgag gcccatcggg
       61 tgctcgcccc cggcggcgcc ctcgcgctct ggacctacaa cctggcccgc gtctcgcccg
      121 aggtggacgc ggtggtcgac cgcctggccc ggcagatcgc cggcccctac tggccgcccg
      181 agcgccgctg ggtcgacgag gagtaccgga ccatcccctt cccctttccc gaggtggaga
      241 tcccaccgtt cgagcacgtg gagcactggg acctcgagcg cctcctcctc tacatcggca
      301 cctggtcggc ctgccagcgc tacctcgccg ccaccggcca ggacccgatc gccctcatcc
      361 gcggcgacat cgaggcggcc tggggaccgg tggaccggcc gcgcaggatc caatggccga
      421 tcttcctgcg cgccagccag ccgatcgaac cctgaaacaa aatcgcgatt tccgcttata
      481 ctcttccgcg cataaatgat cacggcactc gtcagccgga agggcggcgt ggggaagacc
      541 accacggccg tcaacctggc ggccgctctg gcggcgcagg ggcggcgtgt gctcctggtg
      601 gacctggaca gccaggcgtc ggcctcgctg tcgctggggg tgcggcggga ggacctgcct
      661 ccgtcgtcgg ccgacctgct gctgagcggg acgccggcgc gcgaggtgat ccgccggacc
      721 tcggtcgagg ggctggacct gatcaccgcc tcgtcggacc tctcccgcgc cgacctcgac
      781 ctggcgccgt tccgcagccg cgagatccgg ctgcgctcct ccctctccgc ggtggccggc
      841 gactacggct tcgtgctgct ggactgcccg ccgtcgatgt cgctggtgca gatcaacgcc
      901 ctggtggcgg cgcagacgtt catcgtcccg gtgatcccgc agttcctggc ggcggccggg
      961 gtgcgcaacc tgatcgagtc ggcggagcgg ctgagctgga acggcagccg cccggcccgg
     1021 ccgctcggcg tcctcctgac gctggtggac taccggagca aggcgacccg ccagaccgtc
     1081 gaccggctgc gcgccgagta cggcagcctg gtcttcgcca tggagatccg ggtcaacacc
     1141 cgcctcgccg aagcccccgc ggccgggcag accatcttcc agtacgacgc cggggcgacc
     1201 ggcgccgaag cctaccggct gctggccgac gagtacgtgc tgcgcgcggg ggcggcggag
     1261 aggaaaggct agcgcgccgg cttcagagac ggtctgaccc gagttggaga cggctcgacc
     1321 ctcccgaaag cggcctcgac cccagggtcg gacggctcga ccctcccgaa agcggcctcc
     1381 tacggctccg gcacgtagtc cggcgcgggg ccaaaggtcc ccggcgtctc ggcggcggcg
     1441 tagatgaccg cggcccaggc ggcgacgttc tggtccagct ccttggggtc gatcttgtcc
     1501 agcgtgtcgt tggcggtgtg gtgccagtcg aagtagtggg tggcgtcctg gccgaaggcg
     1561 aggacgggga cgtgggcggg ctggaggggg atcaggtcgg cgccgccgct gctctcgttg
     1621 ttgtccccct gttccactcc cagcggacgg aggagctcgg cgagctggcc gacgaagggc
     1681 agcgccgcgg gggccacccg cgaccgcagg cgccagacgc ggccggcgcc gaagtcggac
     1741 tcggccgcca gcacgtgctt cgccagctcg ccggcgtgcg cctcggcata ggcgcgggcc
     1801 cccgagaggc cgaactcctc gttggcgaag aagacgacgc ggatcgtccg ccgggggcgg
     1861 agcggggcga tgcgccgcgc cgcctcggcg acgatgccgc agccggcggc gtcgtccagg
     1921 gcccccgtcc ccaggtccca ggagtcgagg tggcaggcga tcagcacgat ctcctccggc
     1981 cgctcacgcc cccggacctc gccgatgacg ttggccgact cggcctcccc tagcaggcgg
     2041 ctgccgagct tcagccggaa ggtcaccggc ttgcccgagg cgacctgcgc cgccaggagg
     2101 tcggcgtccg ggttcgacag cgccgccgcc gggatccgcg gcaccccctc ctcgtagcgg
     2161 gtgccgccgg tgtggggcac ccggtcgcga tccgtgccga tggagcggat caggacggcc
     2221 accgccccca gccgcgcggc gtgcgacggc ccggcgctgc gtgcggggac ggcgtcgccg
     2281 tagcccttct cgtcgcgggt ccgctccatg cggacgttca ggaagacgat cttcccccgc
     2341 acccgggccg ggtccagggc gtccagcgcc tcgacgctcg ccgcctcgat cacctcggcc
     2401 tcgatcccct cgtccgtggt gccgacgctg ccgcccaggg ccgccagcac caccggctgc
     2461 ggataggggg cgaggatccg ccccgactcc tggccgcgca cccagtgcgg cacggtcacc
     2521 ttctccgccc gcacgttggc gaaccccagc tccttcatct tgcggagcgc ccactcgacc
     2581 gccgccgcgt ccgccttgga cccggccggc cgcggcccga cctccaccac gagcgagcgg
     2641 gcgatctcgt aggcccgggt gccgcgcagg gcctcgtccc gcaacgtggc cgcccgggcc
     2701 cggatctccg ctgtcagact cggaggcgga ctcggagcgg ggccggcatt ctcagccggc
     2761 ggaggcgagg acggcgcgct ctgcagcgcc atggcggaga taccggcgat cccggcgatg
     2821 ccggcgacag tggcgacggc gaggctgacc agagaccgct tcatggcgga tgggctgaca
     2881 ggcatgggct ctcccgaacg tgaagaatgc gccgatgcta acacgcggag gggatggggg
     2941 tggcggggcg gccggtctcg gcccgccccg ccgggagaaa gacgtcaggc ggcggcagaa
     3001 gccggcatct ggtggacctg ctggaggacc tgcttccact catggagagc ctgctccacg
     3061 gcgtggtagg tctgccgcac ccgctcctcg agggacagat gaggctcgcc ggtgccctcc
     3121 gcgccgatgg cggcactggc ggcagccggc tgcttgtgac ggcgctggag cgccaccagc
     3181 tcgtgcaggg cggcctggac ccgggcgtag gcgtcgtgga cgcgctgctc gtagcgctcc
     3241 cgcaaatccg agcccgcctg gatcatggtc tcctgcgcct gctcgaactg cacctccacc
     3301 ttggccttga ggacggcggc ctcggaggcg cggcgcaggt cgcgggcgag acccaccttt
     3361 tcgagcagga agatgatcca cttggccgga tcgaagtgcc aggtcttgat gccgttccgg
     3421 tagtcgaacg ggaagctgtg gtgatagttg tggtagccct cgccgaaggc gaggatcgcc
     3481 atcaggccgc tgtcccgggc gctgtgctcg cggctgtagg gctgccgccc cagcatgtgg
     3541 caggcgctgt tgatgaagaa ggtcgtgtgg tggctggcca cgatccgggc cacgccgccg
     3601 atgagcaggc agccgaggac gcggccggtc gccaggccca cgatcagcgg gatgccgagg
     3661 gcgacgccga tgccgatgag cttgtaccag cggtgctgcc agaccaccag cggatcctca
     3721 tggaggtcgc gcacggcggc ggagttgtcc cgcgggacgt cgtagaagat ccagccgatg
     3781 tgggcccacc agaagccgcg ctggatgttg tacgggtcgc ggtcgtggtc gacgtgggag
     3841 tggtggatgc ggtggtccgc cgcccagttg aggaccgagt tctccagcgc cgcggcgccg
     3901 aacaccagga agaacagccg taccggccag gatgcctgga aggtcttgtg agagaacagc
     3961 cggtgatatc ccgccgtgac cgacagcccg acgcaggtcc agaggaccag gaaggtgacg
     4021 gtctcgggcc agcccaggcc atgcttcacg atgtaccagg gcgtgcccac cagggcgacg
     4081 gcgagggtgc ccaggaggaa gatggtgttg atccagttca ggcgcagttt ttcttcggcc
     4141 actcttctcg ctccacgttc aggctggggt gctggcaccc gggtagacgg tgaacggccc
     4201 ctcccgaaac ggagcgggcg ctccggcgga ggataccatc aggacggcgt ccatagagtt
     4261 caacgttgaa cggcggtgaa accttccccg gcggcccgga caggccaggc agacggcgag
     4321 cgccgcgcgg gatctcggga agcgcattcg gtccgtttca gaaaaggcgg atgcattgta
     4381 ccccggcccc gccggaacgc aaggcccccg gagccctccg ctgccggccg cgccggctcg
     4441 ctgactcaga cggcgacgcc cagcgactgc tggacgaagc cctcgatggc cgcggccggc
     4501 cgggcgccgc tggtgcgcgc cacctcgcgg ccgccctgga agacggccat ggtggggatg
     4561 gagctgacac ggtgcgtggc gcccaccgac gggtcgacgt cggtgttgac cttgaccacc
     4621 aggaagcgcc cggcgttctt cgccgccacc ttctggagct ccggcgccac cgcccggcag
     4681 gggccgcacc agggggccca gaagtccacc agcaccggca gcgaggattc gcgcagcagc
     4741 gccgagagct cgcccccctg atcgaccgcc agcggctcgg ccggcggcgg cagcacggcc
     4801 tggcaggagc cgcagcgcgc ctcggcgccg agacgcgcgt agagcacccg gttcttcttg
     4861 ccgcaggacg ggcaggtccg gatcatccca ttcgacatcg cgaggttccc tccttccaac
     4921 tccacccgga gcaacatcga tgccgcggcc ggcgattccg cgcccccgcc gtgatacctt
     4981 gcccggccgg agggaaggag gcgcgccatg gaggggtacc gggtgaagcc ggggacgaag
     5041 gtggagctga acgggatcga cgcgggctcg accgacgagg ccaagggagg gaagtcgaag
     5101 agcgcggagg ccttcgcgga gctgaaccag aagctcgaaa cgctccagga gatgctctgg
     5161 gcccaggggc gccaccgcgt gctggtggtc ctccagggga tggacaccag cggcaaggac
     5221 ggcgtcatcc gccacgtctt cgaccgggtc aacccgacgg gcgtcaaggt cacctcgttc
     5281 aaggccccca ccgacctgga gctggcgcac gacttcctct ggcgcatcca ctcccaggtg
     5341 ccggcggacg gggagctggt gatcttcaac cgcagccact acgaggacgt cctggtggcg
     5401 cgggtccgga acctggtccc cccggacgtc tggcggcggc gctacgacca gatcaacgac
     5461 ttcgagcggc tgctggccga gaccggcacc accatcctca agttcttcct ccacatcgac
     5521 aaggacgagc agaaggagcg cctccaggcc cgcctcgacg atcccaccaa gcactggaag
     5581 ttccggcgcg gcgacctgga cgaccgccgg ttgtgggacg agtaccagcg ggcgtacgag
     5641 gaggccctct cccgcaccag caccgaccac gccccgtggt acatcgttcc cgccaacagg
     5701 aagtggtacc gc
//
LOCUS       DASPJI010000311        11302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_361799, whole genome shotgun sequence.
ACCESSION   DASPJI010000311 DASPJI010000000
VERSION     DASPJI010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11302)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11302
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361799"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VEL74_08265"
     CDS             complement(<1..1040)
                     /locus_tag="VEL74_08265"
                     /inference="COORDINATES: protein motif:HMM:NF036537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LodA/GoxA family CTQ-dependent oxidase"
                     /protein_id="HYG62563.1"
                     /translation="MPEERTIVRCAIHPALGVARVGNAPADEYYLAPELPDRAADPGP
                     GGYKNAKGQIRKEAARFRVYGYDAEGNVVREITAAEAEITWEVHLANRKAAWYKFFNA
                     MDLAQYALSTTYRNATVAGDLRQALVVDPGPIQISGSGTKGAEYRFEQGFIRFGEGGP
                     IQVPLGELRTDEAGRLLVLGGDGKSGPATAGQAPVTFANNDGWYDDTSDGPVRATVRL
                     HGQSEPLVAEPAMVAVTPPNYGQGLHGVVTMFDVVYDLFVRQGWETKPDRPDFWRHIF
                     PIFERMVNSQWVNQGDYMLFGPGSPGDLLAPDRLAKLADPGPDAKRYRQEIFEMFREA
                     LTPWDEAGGRARL"
     gene            1130..2194
                     /locus_tag="VEL74_08270"
     CDS             1130..2194
                     /locus_tag="VEL74_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HYG62564.1"
                     /translation="MPEPRELPVPDFYDSAHASDWSYQPDQQALFAAALEHRSRHGVR
                     PAATDRRRIHLLLIDVQRDFCFPRGSLYVGGRSGTGAMDDSDRIARFLYRNLEMISEV
                     TCTLDTHYPFQIFSPSFWLGEDGQPPTAHQEITTDDIRSGRMRPNPDIASWVADGDYG
                     WLRRQVEFYCRELERAGKYRLYLWPPHCLVGSDGYALAGVLHEARLFHAWSRGARNGV
                     EIKGGNPLTENYSVLSPEVLKRHDGGPLDEPNTRFIQTLLDADAVVIAGQAASHCVKS
                     TIDDLLGHLLPSDPALAAKVYILEDCMSSVAVPDPGRPGEFVFDFTPQAEDALRRFAA
                     AGMHVVRSTVPMAEWPWIPI"
     gene            3210..3536
                     /locus_tag="VEL74_08275"
     CDS             3210..3536
                     /locus_tag="VEL74_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62565.1"
                     /translation="MSQLGPAWVSLTFACDCTFTSSTRLPWQRIHEQGIYSLEDGVIH
                     FERRGGTTIWPFGIDGASLRLEEAPGEFHSYRRLAEQPCRPSAAISIPDPPLPSRLAA
                     GTPHLS"
     gene            3644..4138
                     /locus_tag="VEL74_08280"
     CDS             3644..4138
                     /locus_tag="VEL74_08280"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYG62566.1"
                     /translation="MNPEMLVAELRSAKEFFDRSTRCLTEEDSSFAPAAGTWTVAQQV
                     AHAAQTVDWFLEGAFRPEGFDTNWDEHQKQVARVQSLGEARAWLEAAFARAIEVLGAR
                     TPEELAQPLPADGIMGGAPRFAIVSAISDHTAHHRGALTVYSRLLGRVPAMPYMDAPA
                     EVTA"
     gene            complement(4135..4809)
                     /locus_tag="VEL74_08285"
     CDS             complement(4135..4809)
                     /locus_tag="VEL74_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HYG62567.1"
                     /translation="MRRADRLFQIVQILRRRRLTTARHVADQLCVSERTVYRDIRDLI
                     ASGVPVEGEAGVGYRLRGFELPPLMFSRDEVEALVLGARIVQSWTDPELGRAAAEALA
                     KIEAVLPGDRSHLVKGTPLFAPGEHLRVELAVDLAVLRQAVREQRRLRFGYTDGAGLA
                     TTRMVRSLALSFYGPIWLLAGWCELREDFRTFRLDRMRDLEVLERVPPEPGRTLQDFL
                     VAVKEC"
     gene            4877..5578
                     /locus_tag="VEL74_08290"
     CDS             4877..5578
                     /locus_tag="VEL74_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG62568.1"
                     /translation="MDSAPLIELRDVTRTYEEGGRTRAVLQGASATIARGELAVLVGR
                     SGSGKSTLLNLLSGIDLPSSGAVIIDGVDISSLSERDRTLFRRDHIGFVFQFFNLIPT
                     LTVVENLLLPLELKGGLDAERRRAALDLLAEVGLADRAGSYPDRLSGGEQQRVAVARA
                     LAHDPLLVLADEPTGNLDLETGLQVLEILDRLTRRAGKTMVMVTHGREVVGLADRVFR
                     VEGGRLVEDFGEVAS"
     gene            5632..8166
                     /locus_tag="VEL74_08295"
     CDS             5632..8166
                     /locus_tag="VEL74_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYG62569.1"
                     /translation="MGLAVLGVALGVAVVVAIDLANASARRAFTLSTEAVTGRTTHQV
                     TGGPAGLPDDAFRRLVLEAGADRAAPVVEEYVMLARQPGQGDRPSRVLHLLGVDPFSE
                     RPFRSYLSVGSPEDGERGSIDLGPLLTRPGAGVLSATTAAELGLRPGDSFRIRVGGGE
                     RPVDLVGVLEPADENTRRAIADLLVTDIATAQELLGRVGRLDRIDLIVPDGPGGEVLL
                     RRAEAVLPAGAQIETAGARSRSTEEMTRSFRLNLTALSLLSLICGVFLIYNTMTFSVV
                     QRRTLLGTLRALGVSRREVFILVLGEALAVGLVGTAAGLGAGIFLGRGLVRLVTQTIN
                     DLYFVVSVTDLALPPLTLAKGAAIGLGATLLAAVAPALEATLAPPRAVLTRSALEARL
                     RRALPRVTALGVGLLAVGGLLLIPRSLMVSFAGLFAVILGCALLAPGATVLLMALLRR
                     PMGRLFGVLGRMAAGGVVASLSRTAVAIAALVIAVSVTVGVGVMIDSFRQTVVRWLEM
                     SLQDDIYVSAPSRAGGFSGSPLTPEVPARAAAVAGVASVQPIRRVEVPSVAGFPGGPL
                     RLVAIDSTAAAFARSFELREGDPREVWPAFLRGEAVVVSEPFSRKRGVEVGSVLRLRT
                     LEGERSFRVAGIYYDYASDQGIVLMSRPVYVRHFRDRSVSGFSVTLAPGADLDATIRR
                     LRTALTGEAVAIQSNVSLKRISLEIFDRTFLITGVLRLLAGLVAFIGVLSALMALQLE
                     RARELGVLRANGLTPGQVWHLITSQTGLMGLAAGLLSIPVGLALAAIMVFVINRRSFG
                     WTIRMEVSPDILLQALLLAMGAALLAGIYPAWKMARTSPALALREE"
     gene            8176..9336
                     /locus_tag="VEL74_08300"
     CDS             8176..9336
                     /locus_tag="VEL74_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HYG62570.1"
                     /translation="MRRILFFPALLAAACLLSCQEPREPAASLSVASALRGGDDRGYA
                     RAVTPRDFQFPADHGPHPGFRTEWWYYTGSLATREGRRLGFQLTFFRSALAPESPERE
                     SAWGANEAWLAHFTVTDAEGGRFVSFERWSRGAVGLAGARAEPFRVWLEDWTAEAAGA
                     RPGQPTPPMRLRAEQDGAAIDLVLQPGKPPVLQGDRGLSQKGPEPGNASYYYSLTRMP
                     ASGTVRVGGERFQVSGLAWMDREWSTSSLSADQVGWDWFALQLDDGTDLMLYRLRKKD
                     GSADPLSSATVAGPAGETRHLSLADFRLEDLERWTSPRSGGVYPARWRVRIPAEDLDL
                     EVSPLLADQELDVSFRYWEGAVAVEGRHRGQPVRGRGYVEMTGYTGEISARH"
     gene            9355..>11302
                     /locus_tag="VEL74_08305"
     CDS             9355..>11302
                     /locus_tag="VEL74_08305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYG62571.1"
                     /translation="MKLFFSRVLPLLSAALLAGVSGVPLMAATPAASAPAARQVKQYT
                     IDQFLAITDYDLASFSPDGRKILVSSNETGVFNAFALPVDGGKPVQLTDSKVSAVRIL
                     GYFPADERFLYSSDQGGDEQTHLYVQAPDGTVRDLTPGDNVRADFLDWAPDGRSFFFT
                     TNERDPKYVDVYEMTVDGYKRTLLFQNDKGYEPSAVSPDRRYVALLDEHNDFDKDVLL
                     YDRESGKLTSILEFPKAEVVNDPGAFTPDGKGLYYLTDEGSEFARLMRYDLASGKSTE
                     LLRPKWDVTNVETSRDGRYLAVGINNDARTEVRLFDTQGMRPLPFPSPAEANVSTVTF
                     SNDGKRVAFYIESSRSPGDLHVLDLSGGGKAEPRRLTRSLTPEIAFEDLVQERVVRFK
                     SFDGVEVPGLLYKPHQAGPDNKVPALVWVHGGPGGQSRVGYHSFIQYLTNRGYAVFSI
                     NNRGSSGYGRTFYSLDDRKHGDADLGDCIASKKMLIDTGWVDPNRIGIAGGSYGGFMV
                     LAALAFKPKEFAVGVNLYGVSNWSRTLESIPPYWEAIRDSLYRELGHPEKDAEYLRRI
                     SPLFHAENIERPMIVLQGEKDPRVLQRESDDIVAAARKRGTPVEYLILPNEGHGFRRK
                     ESQKKAYEAVAAFLDRYLKTAGAAA"
BASE COUNT         1620 a         4158 c         3763 g         1761 t
ORIGIN      
        1 agcctggcgc ggccgccggc ctcgtcccaa ggggtcaggg cctcgcggaa catctcgaag
       61 atctcctgcc ggtagcgctt cgcgtccggg ccgggatccg ccagcttcgc gagccggtcc
      121 ggcgccagca gatcccccgg cgagcccggg ccgaacagca tgtagtcccc ctggttcacc
      181 cactggctgt tcaccatccg ctcgaaaatc gggaagatgt gccgccagaa atccgggcgg
      241 tcgggcttcg tctcccaacc ctggcgcacg aagaggtcgt agaccacgtc gaacatggtc
      301 actaccccgt ggagcccctg gccgtagttc ggcggcgtca ccgcgaccat ggccggctcg
      361 gccaccagcg gctcgctctg cccgtggagt ctgacggtgg cccgcaccgg tccgtccgag
      421 gtgtcgtcgt accagccgtc gttgttggcg aaggtgacgg gtgcctggcc cgccgtcgcc
      481 gggcccgact tcccatcgcc gccgaggacg agaagccgcc ccgcctcgtc agttcgcagc
      541 tcgccgagcg gcacctggat cggccctcct tcgccaaagc ggatgaatcc ctgctcgaac
      601 cggtactccg ctcccttcgt gccgctcccg ctgatctgga taggaccggg atcgacgacc
      661 agggcctggc gcaggtcgcc ggccaccgtg gcgttgcgat aggtggtgga caaggcatac
      721 tgcgccaagt ccatggcgtt gaagaacttg taccaggccg ccttgcggtt tgcgaggtgg
      781 acctcccagg tgatctcggc ctcggcggcg gtgatctccc ggacgacgtt cccctccgcg
      841 tcgtagccat agacgcggaa gcgggccgcc tccttgcgga tctgcccctt ggcgttcttg
      901 tagccgccgg gaccggggtc ggcagcccgg tccggcagct ccggagctag gtagtactcg
      961 tcggcgggcg cgtttccgac gcgcgcgacg ccgagggcgg gatggatggc gcagcggacg
     1021 atggtcctct cttcaggcat gcgagcccct cctctgttca cggaagcttc acctctattg
     1081 tgtcgcgagc cggccgtcta tctcacgatg tgcgccatag aatagcggca tgcccgaacc
     1141 ccgcgagcta ccggtccccg acttctacga ctccgcccac gcctccgact ggagctacca
     1201 gccggaccag caggccctct tcgccgcggc cctggagcac cggagccggc acggggtccg
     1261 ccccgcggcc acggaccgcc ggcggatcca tctcctcctg atcgacgtcc agcgggactt
     1321 ctgtttcccc cggggctcgc tctacgtcgg cggccggagc ggcaccgggg cgatggacga
     1381 cagcgaccgc atcgcccggt tcctctaccg caatctggag atgatcagcg aggtcacctg
     1441 caccctcgac acccactacc ccttccagat cttctccccc tccttctggc tgggagagga
     1501 cggccagccg cccaccgcgc accaggagat caccacggac gacatccgga gtggccggat
     1561 gcggcccaat ccggacatcg cctcctgggt ggcggacgga gactacggct ggctgcggcg
     1621 gcaggtggag ttctactgcc gggagctcga gagggcgggc aagtacaggc tctacctctg
     1681 gccgccccac tgcctggtag ggagcgacgg ctatgctctg gccggggtcc tccacgaggc
     1741 gcggctgttc cacgcctggt cgaggggcgc gcgcaacgga gtggagatca aagggggcaa
     1801 tccgctgacc gagaactact ccgtcctctc tcccgaggtg ctgaagcgcc acgacggcgg
     1861 ccccctggac gagccgaaca cccgcttcat ccagaccctg ctcgacgccg acgcggtggt
     1921 catcgccggc caggccgcca gccactgcgt caagagcacc atcgacgacc tgctggggca
     1981 cctcctcccg tcagatcccg ccctggccgc caaggtctac atcctggagg actgcatgtc
     2041 ctccgtcgcc gtcccggacc ccggccgccc gggcgagttc gtcttcgact tcacccccca
     2101 ggccgaggac gccctgcgcc gcttcgcggc ggcagggatg catgtggtgc ggtcgaccgt
     2161 tccgatggcg gagtggccat ggatacccat ctgaggcggt tgggttgcgg gacaccagac
     2221 gctgtggcca tggggtagac gccccagtcc gcccagcacg gaaggaccgg gtgcactgcc
     2281 gacggggcgt ctcccggttt cagcacagcc atcttccacc cgtcatcctg cggggagcct
     2341 cagcgaccca gaggatctgg gcggggggaa ttcgccctag aagctcagat ttacagaact
     2401 gcggcggccg aatccgccat ttcactatga ccagtcgctc gccgatagaa cgcgcgtctc
     2461 atcgacttgg cgacgcctgt tgtgggcgaa gccatgcccg ttccaggcgc agacgtgccc
     2521 gacgcaggcg tcgccaggca cgcggcaggc atcgtcaggc ctgccgcagg catagccggg
     2581 ccgaccgctg gcgagaccgt gcccgccttg ggcaaagccg ggcctgcggc aggccgcccc
     2641 atgcccgacg caggcgacgt cacgcccgta tcaggcaaag ccgtgcccgc ctagggcgcc
     2701 gagaggcctg cacgtgcaag tgccagcctt gtgtcccgga acgccgagcc gacggggagc
     2761 ctcatcgagc cgcctcgctg tcacgtacag ccgcctggga ggagcatcca gccggaggga
     2821 tacaacgccg agcccggcgg cggacccggg ccgttcgttc cacagggcat gcgatgtact
     2881 cccgcagtac acgaggcgta caggagggac gcccccgcgg accgggcaga ctgcccaagg
     2941 cgagaatctc cagagacttg gctttcagat cataggtggc acggctctcg ctctctcgaa
     3001 tctacgaatc ctcgggagaa gctttcatga ggtcctactc tcgggctgat cagccccgaa
     3061 cgttccgccg gctccgccgc gcgttgacca tcgcggccgt ggctggttcg atgctgacgg
     3121 cctgcacctc tacgcagaca ctcgggcgcc ccgtcgcggg gcgctgtcag cctgatccac
     3181 ggcttaccgg cacctggagg aattaccgct tgagtcagct cggcccggct tgggtctcgc
     3241 tgaccttcgc ttgtgactgc acgttcacga gcagtacccg gctgccctgg cagcgtatcc
     3301 atgaacaggg catctacagc ctcgaggacg gcgtcatcca tttcgaacgg cgaggcggaa
     3361 ccaccatctg gccgttcggt atcgacggcg cctcgctccg cctggaagaa gcgccgggtg
     3421 aattccacag ctaccggcgc cttgcagagc agccttgccg cccttctgct gcgatctcca
     3481 tcccagaccc gcccctccct tccaggctgg cggctggtac tccgcatctc tcctgacagg
     3541 acgctgtcag gagggctatg ggaatctcct cttgccggca gaagccggcc ccggatcacc
     3601 tggccgggac ccgacccatc gacaactcat gagaggagag cccatgaatc ctgaaatgct
     3661 cgtcgcggag cttcgcagcg ccaaggaatt cttcgaccgt tcgacccgct gcctgacgga
     3721 ggaggattct tccttcgccc cggccgccgg cacgtggacg gtggcccagc aggtcgccca
     3781 tgcggcccag accgtggact ggttcctcga aggcgcgttc cgtcccgagg gcttcgacac
     3841 gaactgggac gagcaccaga agcaggtggc ccgggtgcag tcgctcggcg aggctcgcgc
     3901 ctggttggag gcggccttcg cccgggccat cgaggtgctc ggcgcccgca cgccggagga
     3961 gctggcgcag cccctgccgg ccgacgggat catgggcggc gcgccccgct tcgcgatcgt
     4021 ctccgcgatc agcgaccaca ccgcccacca ccgcggcgcg ctgaccgtct actcccgtct
     4081 gctcggccgc gtaccggcga tgccgtacat ggacgcgcct gccgaagtga ccgcctaaca
     4141 ctccttgact gccacgagga aatcctggag cgtgcgcccc ggctcggggg gcacgcgctc
     4201 caggacctcg aggtcccgca tccggtccag ccggaacgtc cggaagtcct cgcgcagctc
     4261 gcaccacccc gcgagcagcc agatcggccc atagaacgag agcgccagcg agcggaccat
     4321 gcgcgtggtg gcgaggccgg cgccatccgt atacccgaag cggagccgcc gctgctcgcg
     4381 gactgcctgc cgcagcacgg ccagatcgac cgcgagctcg acccggaggt gctccccggg
     4441 cgcgaagagc ggcgttccct tgaccagatg gctccgatcg cccggcagga ccgcctcgat
     4501 cttggcgagc gcctccgccg ccgcgcggcc cagctccgga tccgtccagc tctggacgat
     4561 gcgcgccccg agcaccagcg cctccacctc gtcgcgcgag aacatcaagg gaggcagctc
     4621 gaacccccgc aggcgatagc ccacccccgc ctccccctcg accggcacgc ccgaggcgat
     4681 gaggtcgcgg atatcgcggt agaccgtgcg ctccgagacg cagagctggt ccgcgacgtg
     4741 gcgcgcggtg gtcaaccgcc ggcggcggag gatctgcacg atctggaaca gacggtcggc
     4801 gcggcgcatt gggggggcat cttagcgtca gggcagcccg aacaggtgcc cggttctggt
     4861 ctaggatggt ggcctcatgg actccgcccc cctcatcgag ctccgcgacg tcacccggac
     4921 ctatgaggag ggtggacgga cccgggcggt gttgcagggg gcgagcgcca cgatcgcgcg
     4981 cggggagctg gcggtgctgg tggggcggag cggctcgggc aagtccacgc tgctcaacct
     5041 gttgagcggc atcgacctcc cctcctcggg cgcggtgatc atcgacggcg tcgacatctc
     5101 atccctcagc gaacgggacc ggaccctctt ccgtcgcgac cacatcggct tcgtcttcca
     5161 gttcttcaat ctcatcccca ccctcaccgt cgtggagaac ctcctcctcc ccctggagct
     5221 caaagggggc ctcgacgccg aacgccggcg cgccgccctc gacctcctcg ccgaggtggg
     5281 cctcgccgac cgcgccggca gctatcccga ccggctctcc ggaggcgagc agcagcgtgt
     5341 agccgttgcc cgcgccctcg cccacgatcc cctgctcgtc cttgccgacg agcccacggg
     5401 caacctcgac ctcgagaccg ggctccaggt actggagatc ctcgaccgcc tgacccgccg
     5461 ggccggcaag accatggtca tggtcaccca cggccgcgag gtggtggggc tcgccgaccg
     5521 cgtcttccgg gtcgaggggg gacggctggt ggaggacttc ggggaggtgg cctcctgagc
     5581 tccctcctct ggcgctcgag cctccgccac atcctgcgtc acccgtggca gatggggctt
     5641 gccgtcctcg gcgtggccct gggcgtcgcc gtggtcgtgg ccatcgacct cgccaacgcc
     5701 agcgcccggc gggcgttcac cctctccacc gaagcggtca ccggccgcac cacccaccag
     5761 gtgacgggcg gcccggccgg cctacccgac gacgccttcc ggcggctggt gctggaggct
     5821 ggcgccgacc gcgccgcgcc ggtggtcgag gagtacgtga tgctcgcccg gcaacccgga
     5881 cagggggacc ggccgagccg cgtcctccac ctcctcgggg tcgatccttt ttccgagcgc
     5941 ccgttccgct cctacctgag cgtcggttcc cctgaggatg gcgagcgcgg cagcatcgac
     6001 ctcggcccgc tcctcacccg gcccggcgcc ggggtgctct cggccacgac ggcggcggag
     6061 ctgggattgc ggccaggcga ctccttccgc attcgggtag gcggcggcga gcggccggtc
     6121 gacctcgtgg gcgtcctgga gccggcggac gagaacaccc gccgggccat cgccgacctc
     6181 ctggtgaccg acatcgccac cgcgcaggag ctgctcggcc gcgtgggccg gctggaccgg
     6241 atcgacctca tcgtgcccga cggcccgggc ggcgaggtgc tcctccgccg ggctgaggcc
     6301 gtcctcccgg ccggcgccca gatcgagacc gccggcgcgc gcagccggag cacggaggag
     6361 atgacccgct ccttcaggct caacctcacg gccctgtcac tcctctccct gatctgcggc
     6421 gtcttcctca tctacaacac catgaccttc tcggtggtgc agcgccggac gctcctcggc
     6481 accctgcgcg cgctgggcgt ttcgcgccgg gaggtcttca tcctggtcct cggcgaggcc
     6541 ctcgccgtgg gcctggtggg caccgccgcc ggcctgggcg ccggcatctt cctcggccgc
     6601 gggctggtgc ggctggtcac ccagaccatc aacgacctct acttcgtcgt ctcggtgacc
     6661 gatctcgccc tgcccccgct gaccctggcg aagggcgcgg ccatcggcct gggcgccacg
     6721 ctcctcgcag ccgtcgcccc ggcgctggag gcgacactgg cgccgccgcg cgccgtgctc
     6781 acccgctccg ccctggaggc gcggctgcgg cgcgccctcc cccgcgtcac cgcgctgggg
     6841 gtcggcctcc tcgccgtggg tggcctgttg ctcatccccc gcagtctgat ggtgagcttc
     6901 gccggtctgt tcgcggtgat cctcggctgc gcgctgctgg cgccgggggc cacagtgctg
     6961 ctcatggcgc tcctgcgccg ccccatggga aggctgttcg gggtcctggg acgcatggcg
     7021 gccggcggcg tcgtggcgtc cctctcgcgg acggcagtgg ccatcgccgc cctggtcatc
     7081 gccgtgtcgg tcaccgtggg cgtcggcgtg atgatcgaca gcttccggca gaccgtggtg
     7141 cgctggctcg agatgtcgct ccaggacgac atctacgtct cggcccccag ccgggccgga
     7201 gggttcagcg gctcgcccct gactcccgag gtgccggcgc gggccgcggc ggttgccggc
     7261 gtggcgtccg tccagcccat ccgccgggtc gaggtcccct ccgttgccgg attccctggc
     7321 ggtcctctgc ggctggtggc catcgactcc accgcggccg ccttcgcccg ctcgttcgag
     7381 ctgcgggagg gagatccccg ggaggtctgg ccggccttcc tgcgcggcga ggccgtcgtg
     7441 gtctcggagc ccttctcccg caagcgcggc gtcgaggtgg gctcggtgct gcgcctgcgg
     7501 acgctggagg gcgaacggag cttccgggtg gcagggatct actacgacta cgcctcggac
     7561 caggggatcg tgctgatgag ccggcccgtg tacgtccggc acttccggga ccgctccgtc
     7621 tcgggcttct ccgtcactct cgcgccgggg gcggacctcg acgcgacgat ccggcggctg
     7681 cggacggcgc tgaccggcga ggcagtggcg atccagtcga acgtctcgct caagcggatc
     7741 tccctggaga tcttcgaccg caccttcctg atcaccggcg tgctgcgcct gctcgccggc
     7801 ctggtcgcct tcatcggcgt gctctcggcg ctcatggcgc tgcagctcga gcgggcgcgg
     7861 gagctggggg tgctgcgggc gaacggcctc acccccggcc aggtctggca cctgatcacc
     7921 tcgcagaccg gcctcatggg cctggccgcc gggctcctct cgatcccggt cgggctggcc
     7981 ctggccgcga tcatggtctt cgtcatcaat cgccgctcct tcggctggac gatccgcatg
     8041 gaggtctcgc cggacatcct cctccaggcg ctcctccttg ccatgggagc ggccctcctc
     8101 gccggcatct accccgcctg gaagatggcc cggacgtctc cggctctggc gctacgggag
     8161 gagtgaggca ggccgatgcg gcgcatcctg ttcttccccg ccctcctggc cgccgcctgc
     8221 ctcctctcct gccaggagcc tcgcgagccc gcggcgtccc tctccgtcgc gtcggcgctc
     8281 cgcggcggcg atgaccgggg gtacgcccgg gctgtcacac ccagggactt ccagttcccc
     8341 gccgaccacg gccctcatcc cgggttccgt accgagtggt ggtattacac ggggagcctc
     8401 gccacccggg aggggcggcg gctcgggttc cagctcacct ttttccgttc ggcgctcgct
     8461 ccggaatctc ccgagagaga gtcggcctgg ggggccaacg aggcctggct cgcccatttc
     8521 acggtgacgg atgccgaggg cggccggttc gtctcgttcg agcgctggag ccgcggggcg
     8581 gtggggctcg cgggcgcccg ggccgagccg ttccgcgtct ggctcgagga ctggacggcg
     8641 gaggctgctg gcgcccgtcc tggccagccg accccgccca tgcggctgcg ggcggagcag
     8701 gacggcgccg ccatcgacct cgtcctccag ccgggcaagc cgccggtgct ccagggagac
     8761 cgggggctca gccagaaggg gccggagccg ggcaacgctt cctattacta ttcgctgacc
     8821 cgcatgcccg cctcggggac cgtgcgcgtc ggcggcgagc gcttccaggt ctccggcctc
     8881 gcctggatgg accgcgagtg gagcaccagc tcgctctccg ccgaccaggt gggctgggac
     8941 tggttcgccc tccagctcga cgacggcacg gacctcatgc tctatcgtct gcgcaaaaag
     9001 gacggcagcg ccgatccgct gagcagcgcc accgtggccg gtcccgcggg cgagacgcgc
     9061 catctgagcc tcgccgattt ccggctggag gacctggagc gctggaccag cccgcggagc
     9121 ggcggcgtct atccggcacg ctggcgggtc cggatcccgg ccgaggacct ggacctcgag
     9181 gtctcccccc tgctcgccga ccaggagctg gacgtctcct tccgttactg ggagggggcg
     9241 gtggcggtcg aaggccgcca ccgcggccag ccggtccgcg ggcgcggcta tgtggagatg
     9301 acgggataca ctggtgagat cagtgcccgg cactgaacat ccatggaggt tgccatgaag
     9361 ctctttttta gccgagtcct gcccctgctg agcgccgcgc ttctcgccgg cgtctccggg
     9421 gtccccctga tggcggccac gccggccgcc tccgcgcccg ccgcccgtca ggtgaagcaa
     9481 tacaccatcg accagttcct cgccatcacc gactacgacc tggcgtcctt ctcccccgac
     9541 ggccgcaaga tcctggtgag cagcaacgag accggggtgt tcaacgcctt cgccctgccc
     9601 gtcgacggcg gcaagcccgt ccagctcacc gactccaagg tcagcgccgt ccgtatcctg
     9661 ggctacttcc cggcggacga gcggttcctc tactccagcg accagggggg cgacgagcag
     9721 acgcacctct acgtccaggc gccggacggc accgtccgcg acctcacccc gggcgacaac
     9781 gtcagggccg acttcctgga ctgggccccg gacggccgca gcttcttctt caccaccaac
     9841 gagcgcgatc ccaagtacgt cgacgtctat gagatgacgg tcgacggcta caagcggacg
     9901 ctgctcttcc agaacgacaa gggttacgag ccgagcgccg tctcgccgga ccggcgctac
     9961 gtggctctcc tcgacgagca caacgacttc gacaaggacg tcctcctcta cgacagggag
    10021 agcggcaagc tcaccagcat cctggagttc ccgaaggccg aagtcgtcaa cgacccgggt
    10081 gccttcaccc ccgacggcaa ggggctctat tacctgaccg acgaggggtc ggagttcgcc
    10141 cgcctgatgc gctacgacct ggcctccggc aagagcaccg agttgctgcg tcccaaatgg
    10201 gacgtcacca acgtcgagac ctcgcgggac ggccgctatc tcgcggtagg catcaacaac
    10261 gacgcccgga ccgaggtccg gctcttcgac acccagggca tgcgcccgct gccgttcccc
    10321 tcgccggcgg aggcgaacgt gagcaccgtc accttctcga acgacggcaa gcgggtcgct
    10381 ttctatatcg agagcagccg ctcgcccggc gacctccacg tgctggatct ctccggcggg
    10441 gggaaagccg agccccgccg cctcacccgc tcgctcactc ccgagatcgc gttcgaggat
    10501 ctggtgcagg agcgggtcgt ccggttcaag tccttcgacg gcgtggaggt cccggggctc
    10561 ctctacaagc cgcaccaggc cgggccggac aacaaggtgc cggcgctggt ctgggtccat
    10621 ggcggccccg gcggccagtc ccgggtcggc taccacagct tcatccagta cctgacgaac
    10681 cggggctacg cggtcttttc catcaacaac cgtggcagca gcggctatgg ccggaccttc
    10741 tactcgttgg acgaccgcaa gcacggcgac gccgacctgg gcgactgcat cgccagcaag
    10801 aagatgctga tcgacaccgg ctgggtggac ccgaaccgca tcggcatcgc cggcggcagc
    10861 tacggcggct tcatggtgct cgccgccctg gcgttcaagc ccaaggagtt cgccgtcggg
    10921 gtcaacctct acggggtttc caactggtcg cggacgctgg agagcatccc accctactgg
    10981 gaggcgatcc gcgacagcct ttacagggag ctggggcacc ccgagaagga cgcggagtac
    11041 ctgcggcgga tctcgcccct ctttcacgcc gagaacatcg agcgcccgat gatcgtgctc
    11101 cagggcgaga aggacccgcg ggtgctccag cgcgagtcgg acgacatcgt ggccgccgcc
    11161 cgcaagcggg ggacgccggt ggagtacctg atcctcccca acgaggggca cggtttccgc
    11221 cgcaaggaga gccagaagaa ggcctacgag gcggtggcgg cgttcctcga ccgctatctg
    11281 aagacggccg gcgccgcggc tc
//
LOCUS       DASPJI010000312         8871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_825166, whole genome shotgun sequence.
ACCESSION   DASPJI010000312 DASPJI010000000
VERSION     DASPJI010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8871)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825166"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /locus_tag="VEL74_08310"
     CDS             <1..1445
                     /locus_tag="VEL74_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HYG62572.1"
                     /translation="PLGDMAPEEFRRHAHAVVDWMADYLAGVGEYPVLAQVQPGDVAA
                     QLPASPPAEGEPVSEMLADFERIVVPGITHWNHPGFHAYFAISGSAPGILGEMLSAAL
                     NVNAMLWRTSPAAVELEERVCDWLRQMIDLPADFHGHINDTASSSSLVALAAARHRLP
                     GLDIRVRGLAGRADVPPLVVYASEHAHSSIDKACIVLGLGQENVRRLPADAELRMSVP
                     ALAAALEEDRRAGRLPMAVVATVGTTSATSIDPVPEIADLCREHGVWLHVDAAYAGSA
                     AICPEYRALMPGIERADSLVVNPHKWLFTPVDCSVLYVRDPALLRAAFSLVPEYLRTD
                     EPGVSNLMDLGFQLGRRFRSLKLWMVIRAYGVEGLRQRIRAHCAMARAFAARVEEDPG
                     FELAAPVPFSTICFRALSGGDGAAQDRFNERLLARINAAGPFLLSHTALAGRYTLRVA
                     IGNIRTGPEHLETLWDLLVESAAELRGEGA"
     gene            1442..2113
                     /locus_tag="VEL74_08315"
     CDS             1442..2113
                     /locus_tag="VEL74_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HYG62573.1"
                     /translation="MSGFIPYRLARLPEAEMLARARAFHAEMDGRRSVRHFAPDPVPR
                     ELIELAIRTASTAPSGAHRQPWKFVAVSDPVLKREIRTAAEAEEHESYVGGRMPPDWR
                     EALAPMGTSWEKPYLETVPWIVVVFEEMWGVRPDGGKSKNYYPKESTGIACGLFIAAL
                     HRMGLATLTHTPSPMAFLSRLLRRPPNEKPFILFPVGYPAADAEVPDLRRKGLDEVAV
                     WHTGE"
     gene            2207..2983
                     /locus_tag="VEL74_08320"
     CDS             2207..2983
                     /locus_tag="VEL74_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62574.1"
                     /translation="MEDRAQDTPAAGVEVTERGNIYFFYRPAVGAAGEDGPHGLVDVQ
                     RFHLVLSPKGRDTLRVITIGKKRLPEPRDSGQRFWGYVERVADDPDAIVEELEGGREE
                     GRTWLPARPAGEGVYAIACHGNHTHLLYALELPEEPDEVQRELHIGKEGSYLLSVKNP
                     QASSPPSVGLDEEKKAEFPGELQELFGGRRFIACEPPSFLDHEGAELLLMGSADDPGE
                     DLGIDLDPEHETEATADLFQELQMERQRHPVEPLLAGKWA"
     gene            3088..3651
                     /locus_tag="VEL74_08325"
     CDS             3088..3651
                     /locus_tag="VEL74_08325"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HYG62575.1"
                     /translation="MPRITRVYTRTGDDGTTALGGGQRLPKDAPRIEAYGTVDELSSH
                     IGAAVALGLEPRLASELARVQNDLFNLGSDLCILEEDKARMPVPVVEERHVTALERLM
                     DELSSELSPLANFILPGGTPGAAQLHVARTVCRRAERLLVTLAREEPVGAWTVRYLNR
                     LSDALFVMARYQNFRQGAPDVLWDSRA"
     gene            4023..4250
                     /locus_tag="VEL74_08330"
     CDS             4023..4250
                     /locus_tag="VEL74_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011645396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62576.1"
                     /translation="MTSSSNFGLYLTGFIILIIGLALGAHLAGAPTMWIAVGVICLIG
                     IGILSAVKKTQVPRGPNTTGTTNPPGTPPRY"
     gene            complement(4302..6062)
                     /gene="pilB"
                     /locus_tag="VEL74_08335"
     CDS             complement(4302..6062)
                     /gene="pilB"
                     /locus_tag="VEL74_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HYG62577.1"
                     /translation="MAPPPEALRKPAQPQAPDKLGDLLVKSGKINQAQLTEALALQKD
                     QGGRLGTNLVKLGHLNERQLVEALSQQFKVPSVDLNGMEIDEGVVKIIPADIARKYTI
                     FPVTKAGATVTVAMIDPTNVFAMDDVKFMTGYKVEPVVASETAIRAAIDRYYGSTHAI
                     ELKKVMEDLSEESGGDLEVLEEEEDLDLATLEEESEQAPVVKLVNIVLTDAIKRGASD
                     IHIEPYEKDYRVRYRIDGILYEMMHPPLKLKEAITSRVKIMAKLDIAEKRLPQDGRIK
                     IKTKISGKSKDLDFRVSVLPTLFGEKVVMRLLDKDKLMLDMTKLGFEPDSLRRFEQAI
                     LKPYGMVLVTGPTGSGKTNTLYSALQRINTPEVNIMTAEDPVEFNLMGINQVQMKEQI
                     GLNFAAALRSFLRQDPNIVLVGEIRDFETAEVAIKAAMTGHLVLSTLHTNDAPSSINR
                     LMNMGIEPFLVATSVHLVVAQRLVRRICTFCKEPMELPPAGLVNVGFSEHEARTLKLF
                     KGRGCERCSGTGYKGRVGLYEVMEIEDELRELILSGASSYELRQKAMQNGMVTLRLSG
                     LQKIRDGMTTVEEVVRETVL"
     gene            complement(6220..7260)
                     /locus_tag="VEL74_08340"
     CDS             complement(6220..7260)
                     /locus_tag="VEL74_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HYG62578.1"
                     /translation="MRILIVRTSALGDVVHALPVLGALRRHFPEARIGWVVEEAMAPV
                     LAGHPDLDELLTVRLRPWGKAPFQRRTLGELRRFLAALRRFSPDVVLDLMGNHKAGAI
                     SALTLGRRRIGAAKPWRREPSSALWINERVEPRGVHAVDRGLSLLSALGIPDEPADFG
                     GGKIFQAAPGLSTEARAVVDSPGPFALIHPGAGWANKRYRPDWWGEAARLLRQATGVP
                     TWVAAARGEEALAAAAEAASSGAARVVQAPDLPTLAALLRRATLMMGGDSGPTHLAHA
                     LGTPVLMVMGPTDPERHGPYGAPGRVLWRRLPCSFCYKRFDDTRPCLLEIPPDRVARR
                     AAELLSGGAGAP"
     gene            complement(7264..7758)
                     /locus_tag="VEL74_08345"
     CDS             complement(7264..7758)
                     /locus_tag="VEL74_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HYG62579.1"
                     /translation="MGEVLKIEELKERLAGLKREGRTVAFANGHFDLLHVGHLRYLQA
                     ARAEAGEAGALIVAINDDDSVARLKGPGRPVVPASERAELLAALAPVDFVVVFSGDSP
                     APLLAELRPDVHCKGTDYGTPERVPEYPTVRAYGGRTALVGDPKDHATSDLISKVKAL
                     PERE"
     gene            complement(7751..>8871)
                     /locus_tag="VEL74_08350"
     CDS             complement(7751..>8871)
                     /locus_tag="VEL74_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HYG62580.1"
                     /translation="GPCAVEPSRLLQSGPSMPPDLQLLPTTDRLLRLVDAMAGQPVAL
                     LVDLVADLFITGTPKRISREAPVLILSYQGEKLIPGGGANAVANIAALGGRPLPLGVV
                     GDDASGRGLVAALEGLGVDTSGIAVRPGYRTPTKVRILGGGKHSIKQQIVRYDIEETL
                     TLTEEERDRFAGHLARWAGEVRVAILSDYGYGAVEPGFLPRMRAALAGETNEEAKLVC
                     DSRFRLGEFQGLDGATPNEEEAESLFGGEIGDDPERLEEAGGRLLERLGARFLLITRG
                     SRGMSLFRPGLSAHLPVHGTDQVADVTGAGDTVIGTFALALAAGGSPVEAALLANYAG
                     GVVVMKMGTATLTPDELRHAIRADPRPVEEIRWERGNG"
BASE COUNT         1244 a         3130 c         3145 g         1352 t
ORIGIN      
        1 ccccgcttgg tgacatggcg ccggaggagt tccgccgcca cgcgcacgcc gtggtcgact
       61 ggatggccga ctacctggcc ggcgtcggcg agtaccccgt cctagcccag gtgcagccgg
      121 gcgacgtggc cgcacagctc ccggcatccc cgccggcgga gggcgagccg gtctcggaga
      181 tgctggcgga cttcgagcgg atcgtcgtgc ccgggatcac gcactggaat catcccggct
      241 tccacgcgta cttcgccatc tccggctcgg cgccggggat cctgggggag atgctgtcgg
      301 cggcgctcaa cgtcaacgcc atgctctggc gcacctcccc ggccgcggtc gagctggagg
      361 agcgggtctg cgactggctg cggcagatga tcgacctccc cgccgatttc cacggccaca
      421 tcaacgacac ggcctcgtcg agcagcctgg tggccctcgc cgccgcccgc caccggctcc
      481 ccggcctcga catccgcgtc cgcggcctgg ccggccgggc cgacgtcccc cctctggtgg
      541 tctacgccag cgagcacgcc cactcgtcga tcgacaaggc gtgcatcgtc ctggggctgg
      601 gccaggagaa cgtccggcgg ctgccggcgg acgccgagct ccggatgtcg gtgccggcat
      661 tggccgcggc cctcgaggag gaccggcggg ccggccggct gcccatggcc gtggtggcga
      721 cggtggggac cacctcggcg accagcatcg atccggtgcc ggagatcgcg gacctctgcc
      781 gggagcacgg cgtctggctc cacgtcgacg ccgcctacgc cggctccgcg gcgatctgcc
      841 ccgagtaccg cgccctgatg ccgggcatcg agcgtgccga ctcgctggtg gtcaaccccc
      901 acaagtggct gttcaccccg gtcgactgct cggtgctgta cgtccgcgac ccggcgctgc
      961 tgcgcgccgc cttctcgctg gtgccggagt acctccggac ggacgagccc ggggtgagca
     1021 acctcatgga cctcggcttc cagctcggcc ggcggttccg ctccctcaag ctctggatgg
     1081 tgatccgcgc ctatggagtc gaagggctgc gccagcgcat ccgcgcgcac tgcgccatgg
     1141 cccgtgcctt cgccgcccgg gtggaggagg accccggctt cgagctggcg gcgcctgttc
     1201 ccttcagcac catctgcttc cgggcgctgt ccggagggga tggcgcggcc caggaccgct
     1261 tcaacgagcg gctgctcgcc cggatcaacg ccgccgggcc gttcctcctc tcgcacaccg
     1321 cgctcgccgg ccgctacacc ctgcgggtgg cgatcggcaa catccgcacc ggccccgagc
     1381 acctggagac cctctgggat ctgctggtgg agtccgcggc ggagctgcgg ggggaggggg
     1441 catgagcggt ttcatcccct accgcctggc ccgtctgccg gaagccgaga tgctggcccg
     1501 ggcccgcgcc ttccatgccg agatggacgg ccggcggagc gtccgccact tcgcgcccga
     1561 tccggtgccc cgggagctca tcgagctggc catccgcacc gcctccaccg ctccctcggg
     1621 cgcccaccgc cagccctgga agttcgtggc ggtgagcgat cccgtgctca agcgggagat
     1681 ccggaccgcc gccgaggccg aggagcacga gagctacgtg ggcggacgga tgcctccgga
     1741 ctggcgggag gcgctggccc cgatgggcac ttcgtgggag aagccctacc tcgagacggt
     1801 cccctggatc gtcgtcgtct tcgaggagat gtggggcgtc cggccggacg ggggcaagag
     1861 caagaactac taccccaagg agagcaccgg catcgcctgc ggcctgttca ttgccgccct
     1921 ccaccgtatg gggctcgcca ccctcaccca cacgcccagc cccatggcct tcctctcccg
     1981 gctcctccgc cgtccaccca acgagaagcc gttcatcctc ttccccgtgg gctaccccgc
     2041 cgccgatgcc gaggtgccgg acctccggcg caaggggctg gacgaggtgg cggtctggca
     2101 caccggggag tagacggcct cgacaggcgg ccagattacg ctccgttgct gtggcagcga
     2161 gtttgcaaac cactgtcctc gggatgaacg accgaggagc cacgacatgg aagatcgagc
     2221 acaagacacg cccgcggcgg gcgtcgaggt cacggagcgg ggcaacatct acttcttcta
     2281 ccggcccgcg gtgggagcgg cgggggagga cgggccgcac gggctggtcg atgtccagcg
     2341 tttccatctg gtgctgagcc cgaagggaag ggacaccctg cgggtgatca ccatcggcaa
     2401 gaagcggctg cccgagcccc gggacagcgg ccagaggttc tggggctacg tcgagcgggt
     2461 ggcggacgat ccggacgcga tcgtcgagga gctggagggg ggccgggagg aggggcggac
     2521 ctggctgccg gcgcgcccgg ccggcgaggg ggtgtatgcc atcgcctgtc acggcaacca
     2581 cacccacctg ctctatgccc tggagctgcc cgaggagccg gacgaggtgc agcgcgagct
     2641 gcatatcggc aaggagggga gctatctgct gagcgtcaag aacccgcagg cctcgtcgcc
     2701 gccgtcggtc ggcctcgacg aggagaagaa ggccgagttc cccggcgagc tccaggagct
     2761 cttcggcggc cggcgcttca tcgcctgcga gcccccctcc ttcctggatc acgaaggcgc
     2821 cgagctgctc ctcatgggct cggccgacga ccccggcgag gatctgggca tcgacctcga
     2881 ccccgagcac gagaccgaag ccacggccga cctcttccag gagctgcaga tggaacggca
     2941 gcgccacccg gtggagccgc tgctggcggg gaagtgggcc tagggcctcc aacttgaagc
     3001 cggccccggc ctcggatacg gtacgccccg ggtgcgccgg gcgggccggc catcctcttc
     3061 agcttctcag gagccgggag ggcgagcatg ccgcggatca cgcgggtcta tacgcggacg
     3121 ggggacgacg ggacgaccgc tctgggcggc gggcagcgcc tgccgaagga cgctccccgg
     3181 atcgaggcct atgggacggt cgacgagctg agctcccaca tcggcgccgc cgtcgccctc
     3241 gggctcgagc cgcgcctggc ctccgagctg gcgcgggtcc agaacgacct gttcaacctg
     3301 ggctcggacc tctgcatcct ggaggaggac aaggcgcgca tgccggtgcc ggtcgtcgag
     3361 gagcgccacg tcaccgcgct cgagcggctg atggacgagc tgtcgtccga gctgtcgccg
     3421 ctcgcgaact tcatcctccc gggcggcacc cctggggcgg cccagctcca cgtcgcccgt
     3481 accgtctgcc gccgcgccga gcggctgctg gtcaccctgg cccgcgagga gccggtcggc
     3541 gcctggacgg tccgctacct caaccgcctc tccgacgcgc tgttcgtcat ggcgcgctac
     3601 cagaacttcc ggcaaggtgc ccccgacgtc ctctgggaca gccgggcctg aacagagatg
     3661 cgccggcgtc cgggaagctt cccgggccgc gcggaatcca tccatttgaa ttggatcgcg
     3721 tccgagacgt ccgggaagct tcccgaggct cccggactcc atccggatga gctggacgct
     3781 gtccgcagcg ttcggaagct tcccgaggct tccggaatcc atcttttcga cgcggacggc
     3841 atccgggaga cgcgggaagc ttccgcggcg tccggacgag atccacgccg tgcggacgag
     3901 atccacggta tccggatggc cgccggaagc ccgaggttgg cgcggctttt gaattctcag
     3961 ggatctcgtc tgccccgccc gtcgttcggg cggggccctg agatcaacga cgaggaggcg
     4021 ccatgacctc tagctccaat ttcggactct atctgacggg tttcatcatc ctgatcatcg
     4081 gcctggcgct cggggcccat ctggcgggtg cgccgacgat gtggatcgcg gtgggcgtca
     4141 tctgcctcat cggcatcggc atcctgagcg cggtcaagaa gacccaggtg ccgcgcggcc
     4201 ccaacaccac cgggaccacc aaccccccgg gcaccccgcc gcgctactag ccgatccggt
     4261 ccagcgacgg cgggcgggcg cccgccgtcg gtcccatctc tctacagcac ggtctcgcgg
     4321 acgacctctt cgacggtggt catgccgtcg cggatcttct gcaggccgct caggcgcagg
     4381 gtgaccatgc cgttctgcat cgccttctgg cgcagctcgt aggagctggc gccggagagg
     4441 atcagctcgc gcagctcgtc ctcgatctcc atcacctcgt agaggccgac gcgccccttg
     4501 tagccggtgc cgctgcagcg ctcgcagccg cgccccttga acagcttgag cgtccgcgcc
     4561 tcgtgctcgg agaagccgac gttgaccagc ccggccggcg gcagctccat cggctccttg
     4621 cagaaggtgc agatgcggcg caccaggcgc tgcgccacca ccagatgcac cgaggtggcc
     4681 accaggaacg gctcgatgcc catgttcatc aggcggttga tcgatgacgg ggcgtcgttg
     4741 gtgtgcagcg tcgagagcac gaggtggccg gtcatggcgg ccttgatcgc cacctcggcg
     4801 gtctcgaagt cgcggatctc gccgaccagg acgatgttgg ggtcctggcg caggaaggag
     4861 cgcagcgcgg cggcgaagtt gaggccgatc tgctccttca tctggacctg gttgatcccc
     4921 atcaggttga actcgaccgg gtcctcggcg gtcatgatgt tgacctccgg ggtgttgatc
     4981 cgctggaggg ccgagtacag ggtgttggtc ttgcccgagc cggtggggcc ggtgaccagg
     5041 accatgccgt agggcttgag gatcgcctgc tcgaaacggc gcagcgagtc cggctcgaag
     5101 ccgagcttcg tcatgtcgag catgagcttg tccttgtcga ggagccgcat gaccaccttc
     5161 tcgccgaaga gggtgggcag caccgagacc cggaagtcga ggtccttgct cttgcccgag
     5221 atcttggtct tgatcttgat gcggccgtcc tgcggcaggc gcttctcggc gatgtccagc
     5281 ttcgccatga tcttgacgcg gctggtgatc gcctccttga gcttgagcgg cgggtgcatc
     5341 atctcgtaga ggatgccgtc gatgcggtag cgcacccggt agtccttctc atagggctcg
     5401 atgtggatgt ccgaggcgcc acgcttgatg gcgtcggtca ggacgatgtt gaccagcttg
     5461 accaccggcg cctgctcgga ctcctcctcg agggtggcga ggtcgagatc ctcctcctcc
     5521 tccaggacct ccaggtcgcc gccgctctcc tcggagaggt cctccatcac cttcttgagc
     5581 tcgatcgcgt gcgtcgagcc gtagtagcgg tcgatcgccg cgcggatcgc cgtctccgac
     5641 gccaccaccg gctccacctt gtagccggtc atgaacttga cgtcgtccat ggcgaacacg
     5701 ttggtggggt cgatcatcgc cacggtgacg gtggcgcccg ctttggtgac cgggaagatg
     5761 gtgtacttgc gggcgatgtc ggccgggatg atcttcacca ccccttcgtc gatctccatg
     5821 ccgttgaggt cgaccgacgg caccttgaac tgctgggaca gcgcctcgac gagctgccgc
     5881 tcgttcaggt ggcccagctt caccaggttg gtgccgagcc ggccgccctg gtccttctgg
     5941 agggcgagcg cttccgtgag ctgggcctgg ttgatcttgc cgctcttgac cagcaggtcc
     6001 cccagcttgt cgggcgcctg gggctgggct ggcttgcgca gtgcttccgg tggtggtgcc
     6061 atgcgtactc ccgtacaaac cggctccatc ccatgaccgg gacggcagac ggaggtcctt
     6121 caatcagtct ttgagacggc ccgcagtgta gcagtcgcag gggaaagggg caacgtactg
     6181 acccttcgcc atttggcgat gtgcgctcgg gctcgggggt cagggggccc cggccccccc
     6241 ggacaacagc tcggcggccc gccgggcgac ccggtccggc gggatctcca gcaggcaggg
     6301 gcgggtgtcg tcgaaccgct tgtagcagaa gctgcagggg agccggcgcc agaggacccg
     6361 cccgggcgcg ccgtaggggc cgtggcgttc cgggtctgtc gggcccatca ccatgaggac
     6421 cggcgtgccg agggcgtgcg ccaagtgggt cggccccgag tcgcccccca tcatcagggt
     6481 ggcgcgccgc agcagggcgg cgagggtggg caggtcgggg gcctggacca cccgcgccgc
     6541 gccgctggag gccgcctcgg ccgccgccgc cagcgcctcc tcgccgcgcg ccgccgccac
     6601 ccaggtgggg acgcccgtcg cctggcggag gagccgcgcc gcttcccccc accagtccgg
     6661 ccggtagcgc ttgttggccc accccgcccc ggggtggatc agggcgaacg ggcccggaga
     6721 gtccacgacc gcgcgcgcct ccgtggagag gcccggcgcc gcctggaaga tcttgccccc
     6781 accgaaatcc gccggctcgt ccgggatccc cagggcggag aggagcgaca agccccggtc
     6841 gaccgcatgg acgccccgcg gctccaccct ttcgttgatc cagagggcgc tcgacggctc
     6901 ccgccgccag ggcttggccg cgccgatccg ccggcggccg agggtgaggg cggagatcgc
     6961 ccccgccttg tggttgccca tgaggtcgag caccacgtcg ggcgagaagc ggcggagggc
     7021 ggcgaggaac cgccgcagct cgcccagggt gcgccgctgg aacggcgcct tcccccaggg
     7081 gcggaggcgg acggtcagga gctcgtcgag atccgggtgc ccggcgagga ccggcgccat
     7141 ggcctcttcg accacccagc cgatgcgggc ttcggggaag tggcggcgca gcgcgccgag
     7201 caccggcagg gcgtggacca cgtcgcccag ggcgctggtg cggacgatca ggatccgcac
     7261 tcactactcc cgttcgggta gagctttgac cttggagatg aggtcgctgg tggcgtggtc
     7321 cttgggatcg cccaccaggg cggtgcggcc gccgtaggcg cgcacggtcg ggtactcggg
     7381 cacccgctcg ggcgtcccgt agtcggtccc cttgcagtgg acgtccggcc gcagctcggc
     7441 gaggagcggc gccggcgagt cgccggagaa gaccacgacg aagtcgaccg gcgcgagcgc
     7501 cgccagcagc tccgcccgct cggacgcggg gaccaccggc cggcccggac ccttgagccg
     7561 cgccaccgag tcgtcgtcgt tgatcgccac gatcagggcg ccggcctcgc cggcctcggc
     7621 gcgcgcggct tgcagatagc gcaggtggcc gacgtgcagc aggtcgaagt ggccgttggc
     7681 gaaggcgacg gtccggccct cgcgcttgag gccggccagg cgctctttca gctcttcgat
     7741 cttcaggact tcacccattt cctctctccc agcggatctc ctccaccggc cgggggtcgg
     7801 cgcggatggc gtggcgcagc tcatcggggg tgagggtggc ggtccccatc ttcatgacca
     7861 cgacgccgcc ggcatagttg gcgagcaggg cggcctctac aggggagccg ccggcggcga
     7921 gcgccagcgc gaacgtgccg atgacggtgt cgccggcacc ggtgacgtcc gccacctggt
     7981 cggtgccgtg gaccggcagg tgggccgaga ggcccggccg gaacaggctc atgccgcggc
     8041 tgccgcgggt gatcaggagg aagcgggcgc cgagccgctc gagcagccgc ccgccggcct
     8101 cctccaggcg ctccgggtcg tcgccgatct cgccgccgaa gaggctctcg gcctcctctt
     8161 cattgggggt ggcgccgtcg agcccctgga actcccccag ccggaagcgg ctgtcgcaga
     8221 ccagcttcgc ctcctcgttc gtctctcctg caagcgccgc ccgcatccgc ggcaggaagc
     8281 cgggctcgac cgcgccgtag ccgtagtccg agaggatggc cacccgcacc tcgcccgccc
     8341 agcgcgccag gtgcccggcg aaccggtccc gctcctcctc ggtcagggtc agcgtctcct
     8401 cgatgtcgta gcggacgatc tgctgcttga tggagtgctt gccgccgccg aggatccgca
     8461 ccttggtggg ggtccggtag ccggggcgga cggcgatgcc ggacgtgtcg acgccgagcc
     8521 cctccagcgc cgcgacgagc ccgcggccgc tggcgtcgtc gcccaccaca cccaggggga
     8581 gggggcggcc gccgagggcg gcgatgttgg ccacggcgtt ggcgccgccg ccagggatca
     8641 gcttctcccc ctgataggag aggatgagca ccggcgcctc gcggctgatc cgtttcggcg
     8701 tgccggtgat gaacaggtcc gccaccaggt cgaccagcag cgcgaccggc tgcccggcca
     8761 tggcgtcgac caggcgcagc agccggtcgg tggtcgggag cagttggaga tcggggggca
     8821 tcgagggccc ggattgtagc aagcgcgagg gctcaaccgc gcatggacct c
//
LOCUS       DASPJI010000313         4658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_419357, whole genome shotgun sequence.
ACCESSION   DASPJI010000313 DASPJI010000000
VERSION     DASPJI010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419357"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VEL74_08355"
     CDS             complement(<1..427)
                     /locus_tag="VEL74_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYG62581.1"
                     /translation="MERLSFSGYSTRDVATLLGLSADQVRSYVRAGFLAPRLGTRGEY
                     LFSFQDLILLRTAKGLLDAQVPPRRIRLALRKLHDQLPEGKPLSGVRITAQGHQVVVR
                     DGGEVWHPESGQILFDFGVSDLARDAASITRHDGEERRSR"
     gene            complement(466..1401)
                     /locus_tag="VEL74_08360"
     CDS             complement(466..1401)
                     /locus_tag="VEL74_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HYG62582.1"
                     /translation="MRMSARPFASGTVSFGLVNVPVKLYSTGESAAGIQLNMLHSKCG
                     SRLRQQYICPTDNAVVGRDEMVKGYEYAKGQYVLFTEEELKALTPEATNAIEITEFVP
                     LEEVDPIYFEKSFYLGPDKGGDRPYRLLAEAMKLTGRAALARYAARGKDQLVLLRPFE
                     GGLIMQQLRYSDELRPFSEVPLVPAEVKEQELKLAMQLIEQIASDEFKPEDYEDTVRK
                     RTRGLIEQKIQGQEITAAPPEAPKAQIIDLMEALKASLSAAQPQTAKAPEKTVSLASR
                     KPPKPSSRRPAVAANSANSEEGEVAAEPKKKSSRR"
     gene            1550..3526
                     /gene="ligD"
                     /locus_tag="VEL74_08365"
     CDS             1550..3526
                     /gene="ligD"
                     /locus_tag="VEL74_08365"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HYG62583.1"
                     /translation="MKPETCDQPFTRPGWLFELKYDGFRLLARRDAEGGARLLYRSGR
                     DALRYFPEVGRDVAALPWEGVILDGEVVVLDERGRPSFQRLQRRGVHARPVESLLTAD
                     PAVYFVFDILALEGHDLRPLPLVTRKEVLRRVLPADGPLRYVEPVAERGEDFYEAVSR
                     MGLEGIVAKKADSPYRAGYSADWQKIRVDQVADFAVVGFTPVPGTRTGFRRLHLAVHE
                     GQGFRYAGRVGSGFSAAEYDEIRARLDTARRVRPRLTGPVPEGPGPVWVEPELVAEVR
                     YKEWTEEGQLRQPIFLRLRDDKTVYECFRPGDEPEGDTAGGPGGPGGPGGDRPAQATG
                     SVGDGEGGAHTKEPSRADPPQAPPPSPRFTNLTNLEKLFWPEEGYTKGDLIDYYRAVS
                     PWMLPYLEDRPLVLDRYPDGIAGKSFFQKSAPEGARQLGDRLRTLPIRPEGSGRAIDY
                     FLCDDEASLLFLVNLGAIPFHVWASRALSLDRPDWCILDLDPKGAPFANVVRIALAIR
                     ELCAAIELPSFIKTSGGSGLHVLLPMGRQLDHGQVRQLAELLAGVIAGRLPEIATTAR
                     AISKRRGRVYVDALQNGRGKLLVAPLSVRPLPGATVSTPLRWEEVGPDLDVRRFTIRS
                     VPERLRGQEEDPLLPVLGQVPDLARSLELLSGLL"
     gene            complement(3949..>4658)
                     /locus_tag="VEL74_08370"
     CDS             complement(3949..>4658)
                     /locus_tag="VEL74_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62584.1"
                     /translation="YYVIRAYNGCESVSSTQVSAATAICPPCSTQTLYSNGFETGTGL
                     NGFSTGTFVSGGATTDWRGTQSCTAKTGTKIFRFGGSNCTANYGNGRFAFAQANGATG
                     IAVPAGSSNTRLSFWHRRRFETGYDGGTIAVSVNGTNYFLAASTAIVSGTVFNGTVNA
                     ACAPTGAAGLPIFTGVATSFVNTVLDLDAVCNAATGLTTGCAGQSVRVAFTSVSDCST
                     NDDGWFIDDVAVTACVP"
BASE COUNT          697 a         1540 c         1686 g          735 t
ORIGIN      
        1 gccggctccg gcgctcctcg ccgtcgtgcc gggtgatgga ggcggcgtcg cgggcgaggt
       61 ccgagacgcc gaagtcgaac aggatctgcc cggactccgg gtgccacacc tcgccgccgt
      121 cgcgcaccac cacctggtgc ccctgggccg tgatgcggac gccggacagc ggcttcccct
      181 cggggagctg gtcgtggagc ttgcggagcg ccagccggat gcgccgcggc ggcacctggg
      241 cgtccaggag ccccttggcg gtgcgcagca ggatcaggtc ctggaaggag aagaggtatt
      301 cgccgcgggt gcccaggcgg ggggcgagga agcccgcccg cacgtacgag cggacctggt
      361 ccgccgagag gcccaacaag gtggcgacgt cgcgggtgga atagccactg aacgacaggc
      421 gttccatcgc gcccagtgta ctacttcacg acccgcgagg ccgtgttacc gcctggagct
      481 cttctttttc ggctcggccg caacctcgcc ctcctcgctg ttggcgctgt ttgcggcaac
      541 ggcgggccgc cgggacgacg gcttgggcgg cttgcggctg gcgagcgaga cggtcttctc
      601 gggcgccttg gcggtctgcg gctgggccgc ggagaggctc gccttgagcg cctccatgag
      661 gtcgatgatc tgggccttgg gggcctcggg cggcgccgca gtgatctcct ggccctggat
      721 cttctgctcg atgaggccgc gcgtccgctt gcggaccgtg tcctcgtagt cctcgggctt
      781 gaactcgtcg ctcgcgatct gctcgatgag ctgcatggcg agcttgagct cctgctcctt
      841 gacctcggcc ggcaccaggg gcacctcgga gaaggggcgc agctcgtccg agtagcggag
      901 ctgctgcatg atcagcccac cctcgaaggg gcggagcagc acgagctggt ccttgccgcg
      961 ggcggcgtag cgcgccaggg cggcgcggcc ggtcagcttc atcgcctcgg ccagcagccg
     1021 gtagggccgg tcgcccccct tgtccgggcc gaggtagaag gacttctcga agtagatcgg
     1081 gtcgacctcc tcgagcggca cgaactccgt gatctcgatg gcgttggtcg cctccggggt
     1141 cagggccttg agctcctctt ccgtaaacag aacatactgg cccttggcgt attcgtaacc
     1201 tttcaccatt tcatctctgc ccacgaccgc gttgtcagtc ggacagatat attgctggcg
     1261 aaggcgcgaa ccgcacttcg agtgcaacat gttgagttgg atgccggcgg cgctttctcc
     1321 ggtagaataa agcttcaccg ggacattcac gaggccgaac gagacggttc cggatgcgaa
     1381 ggggcgagcg ctcattcgca taatatacca ttgttttcca atggttgcgc gccgcaggga
     1441 tcgctccgga aaatctgaag cattcgcttc atcggcggcc gaggtccggg attggctggc
     1501 acgtctcggc gcgccacgcg aagacgtgga gctgggccag gtgtcggcga tgaagcccga
     1561 gacctgcgac cagccgttca cccggccggg ctggctcttc gagctcaagt acgacggctt
     1621 ccggctcctc gcccgccgcg acgcggaagg aggagcccgc ctcctctatc gcagcggccg
     1681 ggacgccctg cgctacttcc ccgaagtcgg ccgcgacgtg gcggcgctcc cctgggaggg
     1741 ggtgatcctg gacggcgagg tggtggtgct ggacgagcgg gggcggccga gcttccagcg
     1801 cctccagcgc cggggtgtcc acgcgcggcc ggtcgagtcc ctgctcacgg cggacccggc
     1861 ggtctacttc gtcttcgaca tcctggcgct cgagggccac gacctgcgcc ctctccccct
     1921 cgtcacgcgc aaggaggtcc tgcgccgggt gctgcccgcg gacggcccgc tccgctacgt
     1981 cgagccggtg gccgagcggg gggaggactt ctacgaggcg gtgtcgcgca tgggcctcga
     2041 gggcatcgtc gccaagaagg ccgactcccc ctaccgcgcc ggctattcgg cggactggca
     2101 aaaaatccgt gtcgaccagg tggccgactt cgccgtcgtg ggcttcaccc cggtgccggg
     2161 cacgcgcacg ggcttccggc ggctccacct cgccgtccat gagggacagg gcttccgcta
     2221 cgcgggccgg gtcggcagcg gcttcagcgc cgccgagtac gacgagattc gcgcccggct
     2281 cgacacggcg cgccgggtcc ggccgcgtct caccgggcct gtccccgaag gtccgggccc
     2341 ggtctgggtg gagcccgagc tggtcgccga ggtgcgctac aaggagtgga ccgaggaggg
     2401 ccagctccgc cagcccatct tccttcgcct ccgtgacgac aagaccgtct acgagtgctt
     2461 ccggccgggg gacgagccgg agggcgacac cgccggcggg cctggagggc ctggggggcc
     2521 tgggggggat cgccccgcgc aggcgactgg cagcgtgggg gacggggagg ggggagcgca
     2581 cacgaaggag ccgagcaggg ccgatccccc ccaggccccc ccgccatcgc cccgcttcac
     2641 caacctcacc aacctggaga agctcttctg gcccgaggag gggtacacca agggggacct
     2701 gatcgactat taccgggccg tctccccctg gatgctgccc tacctcgagg accggccgct
     2761 ggtcctcgac cgctaccccg atggcatcgc cggcaagtcg ttcttccaga agagcgcccc
     2821 ggaaggagcc cgccagctcg gggaccgcct gcgcaccctg cccatccgcc cggaggggag
     2881 cgggcgcgcg atcgactact tcctctgcga cgacgaggcc tccctcctct tcctggtcaa
     2941 cctcggggcc atccccttcc acgtctgggc gagccgcgcc ctgtccctgg accggccgga
     3001 ctggtgcatc ctcgacctcg accccaaggg ggcgccgttc gccaacgtgg tgcggatcgc
     3061 cctcgccatc cgggagctgt gcgccgcgat cgagctcccg agcttcatca agaccagcgg
     3121 cggcagcggc ctgcacgtgc tgttgccgat gggacggcag ctcgatcacg ggcaggtgcg
     3181 gcaactcgcc gagctgctgg cgggggtgat cgccggccgg ctgccggaga tcgccaccac
     3241 cgcgcgcgcc atttcgaagc gccgcgggcg ggtctacgtc gacgccctgc agaacggccg
     3301 cggcaagctc ctcgtcgcgc ccctctccgt ccgtcctctc ccgggggcca cggtgtcgac
     3361 gccgctgcgt tgggaggagg tgggcccgga cctcgacgtg cgccggttca ccatccgctc
     3421 ggtgccggag aggttgcgcg ggcaggagga ggatcccctg ctgccggtcc tcggccaggt
     3481 cccggacctg gcgcgctccc tcgagctgtt gtcgggactg ttgtagagaa aaccgtctga
     3541 aagaaagaga gacggcgcca ccgtgggggc gatggcggcc gcctcgactc gatgagaccc
     3601 gatcaacaac gccctcaaag ggtgttgatc ggccggctcc cgatctcctc ccggtcgctg
     3661 atggggggga acggtccagg aggagaacgg agccggtgct agatagggca agggcgatgc
     3721 cagcgcgggc cttgagagaa tagctaatgg aatcaatagt ttggaaagac agctcggcag
     3781 gggtggctcg atttcgaacc cacttggctg ggtcagattc tggtcagtgg gtcaacaatt
     3841 gccccgcctt ccagcttctc tataccctga agaacgaagc ggcaccccga agggtgccgc
     3901 gcgagaagga agggcggccc tctcgggaga gagggccgcg aagcggcgtt acggcacgca
     3961 ggccgtcacg gcgacgtcgt cgatgaacca accgtcgtcg ttggtggagc agtcgctgac
     4021 cgaggtgaag gcgacacgga ccgactggcc ggcgcagccg gtggtgaggc cggtggccgc
     4081 gttgcagacc gcgtcaaggt cgagcacggt gttgacgaag gaggtcgcca cgccggtgaa
     4141 gatcggcagg ccggcggcgc ccgtgggagc gcaggcggcg ttcacggtgc cgttgaagac
     4201 ggtgccggag acgatcgcgg tggaggccgc gaggaagtag ttggtgccgt tcacggagac
     4261 ggcgatggtg ccgccgtcat agccggtctc gaaccggcgg cggtgccaga aggagaggcg
     4321 ggtattgctg gagcccgcgg gcacggcgat gccggtggcg ccgttggcct gcgcgaaggc
     4381 aaaccggccg ttgccgtagt tggcggtgca gttgctgccg ccgaaccgga agatcttggt
     4441 gccggtcttg gcggtgcagc tctgggtgcc gcgccaatcg gtggtggcgc cgccggagac
     4501 gaaggtgccg gtgctgaagc cgttgaggcc ggtaccggtc tcaaagccgt tgctgtacag
     4561 cgtctgcgtc gagcacggcg ggcagatcgc ggtggccgcc gagacctggg tggagctcac
     4621 cgactcgcag ccgttgtagg cgcggatcac gtagtaat
//
LOCUS       DASPJI010000314         4298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_192513, whole genome shotgun sequence.
ACCESSION   DASPJI010000314 DASPJI010000000
VERSION     DASPJI010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192513"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2040
                     /locus_tag="VEL74_08375"
     CDS             <1..2040
                     /locus_tag="VEL74_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HYG62585.1"
                     /translation="GEEPLRGRTPATEAALLEAAALAEDVRALHEREGIPWKEAAILL
                     RSTGDLDVYLEALRRAGVPFVVGRDKQYYRRREVIEAAALVRAVLDPGDHLALLTVLR
                     SSMVGVPDAALIPLWRRELPRRMTELASPDPERLAVLCELIEAAARETPRDVPGIERV
                     AGWETSLLAAVEHLALLRESFATDPADVFVERLRRLTLVEAVEAARYLGPYRLANLER
                     FFRQLLAAIEEGGGDVAAILRSLRKSVAEAREAEEGRPQDGAEDAVSVLTIHGAKGLD
                     FGHVYLVQLHKTSAGENGDKTEVGRIEDPFAQPGEGTRIEYRLFGAPTLGFDQVEGAR
                     REVESAERVRTLYVAMTRAKDRLVLAGVWPDEALSPVARPAELARTHIDLLLSRPERP
                     DGLALLWGEGAETGRWVFPDPTGALWKLPGLRPEADGLWREEAQGPPLATPGEVARAS
                     EELAARRAEALSRMERSFSGAASEEAHELLREQMTALRLGEDGGARRSASTPEDREAA
                     MAAGSAVHRALETWDLAADPRVELQRQTALLAATLSTLAGGEVLEAALPRARDLLDRF
                     ARGPLLERLRRLRDHVIARELPVLLPPPDREGAPVGFVSGAIDLLYKDPETGRLVIAD
                     YKTDEVETAEHVAARAAVYAPQGAVYVRALREALDLAEAPRFELWFLAVGRTLTP"
     gene            2835..>4298
                     /locus_tag="VEL74_08380"
     CDS             2835..>4298
                     /locus_tag="VEL74_08380"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYG62586.1"
                     /translation="MKKNLSLALLLSLVLSACAAGPYAATPAAMPSVAPAASAPVQTA
                     AAAPAPAIAPERLAQDLPFDKNLIKGKLDNGLTYYVKRNPRPEKRAEVWLAVDAGSVL
                     EDEDQRGLAHFIEHMAFNGTKRFEKMELVNYLESIGMQFGADVNASTSFDETIYTLRV
                     PTDRPEVMKKAFEILEDWAGAVSFDADEVNKERGVVIEEWRLGRGAEARIEDKQLPIL
                     FKGSRYAERLPIGTLEVLEKATPEQLRRYYKDWYRPDLMAVVVVGDIDPQQMEAMVKE
                     HFGGLKNPDNPRPRQLYDVPTGGGTRVAITTDPEATDTTVGIYSVIDKRPEGRVGDYR
                     QSLIENLYHDMLNSRLRELTQGGVPPFSWAGSSAGRFVRTRDVLFQMVAVPEGGIQEG
                     LEALLTEVERVRRHGFTATELERAKKELILFYDQAEQENGKLDSANFAAEYLRNFFED
                     EPVPGIAYEVELVREVMPTIQLPEVNRLAEEWTGGDRV"
BASE COUNT          610 a         1568 c         1543 g          577 t
ORIGIN      
        1 ggggaggagc cgctccgcgg gcggactccg gccaccgagg ccgcgctgct cgaagccgcg
       61 gcgctggccg aggacgtccg cgcgctgcac gagcgtgagg gcatcccgtg gaaggaggcc
      121 gccatcctgc tgcgcagcac cggcgacctc gacgtctacc tggaggcgct gcgccgcgcc
      181 ggcgtcccct tcgtcgtcgg ccgcgacaag cagtactacc ggcggcgcga ggtgatcgag
      241 gcggcggcgc tggtgcgggc ggtcctcgat cccggggatc acctggcgct actcaccgtc
      301 ctgcgttcgt ccatggtggg ggtgccggac gccgccctga tccccctctg gcggcgggag
      361 ctgccgcggc ggatgaccga gctggcctcc ccggacccgg agcggctggc cgtcctgtgc
      421 gagctgatcg aggcggcggc ccgcgaaacg ccgagggacg tccccggcat cgagcgggtc
      481 gccggctggg agacgagcct gctggcggcg gtggagcacc tggcgctcct ccgcgagtcg
      541 ttcgccaccg accccgcgga cgtcttcgtc gagcggctgc ggcggctgac gctggtcgag
      601 gccgtggagg cggcgcgcta cctcgggccc taccggctgg cgaacctgga gcgcttcttc
      661 cggcagctcc tcgccgccat cgaggagggg ggaggggacg tcgccgccat cctgcggtcg
      721 ctgcgcaaga gcgtggccga ggcgcgggag gcggaggagg ggaggccgca ggacggcgcc
      781 gaggacgcgg tgagcgtcct caccatccac ggcgccaagg ggctggactt cgggcacgtc
      841 tacctggtgc agctccacaa gacctcggcg ggggagaacg gcgacaagac cgaggtcggc
      901 cggatcgagg acccgttcgc gcagcccggc gaggggacgc gcatcgagta ccggctcttc
      961 ggcgcgccga cgctcggctt cgaccaggtg gagggggcgc gccgcgaggt ggagtccgcc
     1021 gagcgggtgc ggacgctgta cgtcgccatg acccgtgcca aggaccgcct ggtgctggcc
     1081 ggcgtctggc ccgacgaggc cctgtcgccg gtcgcgcggc cggccgagct ggcgcggacg
     1141 cacatcgacc tcctcctctc gcggcccgag cgcccggacg gactcgccct cctctggggc
     1201 gagggggccg aaaccggccg ctgggtcttc cccgatccca ccggcgcgct ctggaagctc
     1261 ccgggcctgc ggccggaggc ggacggcctc tggcgcgagg aggcgcaggg gccgccgctc
     1321 gccacccccg gggaggtcgc ccgggcgtcc gaagagctgg ccgcccgccg cgccgaggcg
     1381 ctgtcccgga tggagaggag cttctccggc gccgcctcgg aggaggcgca cgagctgctg
     1441 cgcgagcaga tgacggccct ccggctcggc gaggacggag gggcccggcg ctccgcctcg
     1501 acccccgagg accgggaggc cgccatggcc gccggctccg cggtccaccg cgccctggag
     1561 acctgggacc tcgccgccga cccgcgcgtc gagctccagc gccagaccgc cctcctcgcc
     1621 gccaccctct ccaccctcgc cggaggggag gtcctggagg ccgccctgcc gcgggcccgg
     1681 gacctcctgg accgcttcgc cagggggccg ctcctggagc gcctccgccg cctgcgggac
     1741 cacgtcatcg cccgggagct gccggtgctc ctgccgccgc ccgaccggga gggggccccc
     1801 gtcggcttcg tctcgggagc gatcgacctc ctctacaagg acccggagac cggccgtctc
     1861 gtcatcgccg actacaagac cgacgaggtg gagaccgccg agcacgtcgc cgcccgcgcc
     1921 gcggtctacg cgccgcaggg ggcggtctac gtccgggcgc tgcgggaggc gctggacctg
     1981 gcggaggcgc cgcgcttcga gctctggttc ctggcggtgg ggcggaccct caccccctga
     2041 ccccaacgcg ctgccggaag cttccaacgc gctggcgaag cttccaacgg cccgtcggag
     2101 tcttcggcgc gctgtcgaag cttccaacgg gctatcgaag tctccgatgg gctgccggag
     2161 cttccgactc ggtatcgaag cttccggcgc cccatcggag ccttcggcag gctgtcgaag
     2221 gttccggcgt gccgtcggag tctccggcgg gctgccggaa gcttccaatg cgctgtcgaa
     2281 gcttccgatg gcccattgga gccttcggcg ggctgtcgaa ggttccggcg cgctggcgga
     2341 gtctccggcg ggccgacgga agcttccaat ccgccgccgg agatcccggc gcgccgtcgg
     2401 agcctccgcc aagagaaaag cgccttcgag gaagcgcccg gtcgccgcgg atgcgggacg
     2461 gggcggtcgg atgccatcca ggcgccgtgg atctcgtcca ggcgccgtgg aggtcatccg
     2521 gaccgcccgg gaagctcccg ggcgctgcgg atcgcgtcca actcgtcggg atggaatccg
     2581 gagggcccgg gaagctcccg agcgctttgg atggcatcca gttcaaccgg atggattcca
     2641 gacgctccgg gaagcttccc gagcgttccg gatggcgtcc ggttcaatcg gatggagtcc
     2701 gggagcgtcc ggaagcctcc ccctctcccg gtgagtgccg gcctggaaca cgcccccgcc
     2761 cccgcgtgtt gttccttcca cgggtcccac ccatctggac ggcggtcccg ctaacttcga
     2821 tccttcagag gcctatgaag aagaacctct ctctcgccct cctgctctcc ctggtcctct
     2881 cggcctgcgc ggccggcccc tacgccgcga cgccggcggc gatgccgtcc gtggctccgg
     2941 cggcctccgc tccggttcag accgccgccg cggctccggc gccggccatc gcgcccgagc
     3001 ggctggctca agacctcccc ttcgacaaga acctgatcaa ggggaagctc gacaatggcc
     3061 tgacctacta cgtcaagcgc aacccgcggc cggagaagcg ggccgaggtc tggctcgcgg
     3121 tcgacgccgg gtccgtgctg gaggacgagg accagcgcgg gctcgcccac ttcatcgagc
     3181 acatggcctt caacggcacc aagcggttcg agaagatgga gctggtcaac tacctcgagt
     3241 cgatcggcat gcagttcggc gccgacgtca acgcctccac cagcttcgac gagaccatct
     3301 acaccctgcg cgtgcccacc gaccggcccg aggtgatgaa gaaggcgttc gagatcctgg
     3361 aggactgggc gggcgccgtc agcttcgacg ccgacgaggt caacaaggag cgcggcgtgg
     3421 tcatcgagga gtggcggctc ggccgcggcg ccgaggcccg catcgaggac aagcagctgc
     3481 ccatcctctt caagggctcc cgctacgccg agcgcctgcc catcggcacc ctggaggtcc
     3541 tcgagaaggc cacccccgag cagctccgcc gctactacaa ggactggtac cggcccgacc
     3601 tgatggccgt ggtggtcgtc ggcgacatcg acccccagca gatggaggcg atggtcaagg
     3661 agcacttcgg cggcctcaag aacccggaca atccgcgccc gcggcagctc tacgatgtcc
     3721 ccaccggcgg cggcacccgc gtcgccatca ccaccgaccc cgaggccacg gacaccaccg
     3781 tcggcatcta ctcggtgatc gacaagcgcc ccgaggggcg ggtgggcgac tatcgccagt
     3841 ccctgatcga gaacctctac cacgacatgc tgaactcgcg gctgcgcgag ctgacgcagg
     3901 gcggcgtgcc gccgttcagc tgggcggggt cgagcgccgg ccgcttcgtc cgcacccgcg
     3961 acgtcctctt ccagatggtc gccgtgcccg agggggggat ccaggagggg ctcgaagccc
     4021 tgctcaccga agtcgagcgg gtgcgccgcc acggcttcac ggccaccgag ctcgagcggg
     4081 cgaagaagga gctgatcctg ttctatgacc aggccgaaca ggagaacggc aagctcgact
     4141 cggcgaactt cgccgccgag tacctgcgga acttcttcga ggacgagccc gtgcccggca
     4201 tcgcctacga ggtcgagctg gtgcgcgagg tgatgccgac catccagctc cccgaggtca
     4261 accgcctcgc cgaggagtgg accggcggcg accgggtg
//
LOCUS       DASPJI010000315         3264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_651020, whole genome shotgun sequence.
ACCESSION   DASPJI010000315 DASPJI010000000
VERSION     DASPJI010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651020"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VEL74_08385"
     CDS             <1..555
                     /locus_tag="VEL74_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYG62587.1"
                     /translation="LNVSARLFSDPSLAGRVQTALQEAHAGGERLVLEITESALMEEP
                     ETATEVLSRIRTQGVQVAIDDFGTGYSSLSYLQQFPIESLKIDRSFIARVGEQGENAE
                     ILRAILTLARSLGIRVIAEGVETGEQLAQLVAMGCDMAQGYLFSPPVDAGTARALLAQ
                     PPAWAGLLRPFCPRPALKIVGRGF"
     gene            582..1121
                     /locus_tag="VEL74_08390"
     CDS             582..1121
                     /locus_tag="VEL74_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYG62588.1"
                     /translation="MASEILQWPLTTAFAAEQRAARLGNILEETTDAVLMKDLAGRYH
                     FANRAAALLLGRGPHEVVGRSDAELLAADAAAAVRDVERRVLDAGRTLVHEETRTVDG
                     LLRQCVSTRGVFRNVHGEVTGLFCVTRAAGGSREVEAEVVSLAAEVETPQEAALILRP
                     FHLHAVAALLRQRRDRQAS"
     gene            1183..1494
                     /locus_tag="VEL74_08395"
     CDS             1183..1494
                     /locus_tag="VEL74_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62589.1"
                     /translation="MHTVTEVWGREWEEHSLPLFELLQRAERGGVTDICQLLRHVRHC
                     RTTLTEAPTPRLQGLAVRTLDELWEAARLTLYGDLGLADEHTAAAWGHAQKIHQLLSD
                     C"
     gene            1656..2213
                     /locus_tag="VEL74_08400"
     CDS             1656..2213
                     /locus_tag="VEL74_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62590.1"
                     /translation="MDAVADPECAIGGGPLDPRSFLGILSGLSNEDDGESGATLLLLL
                     LRPRWGEAAPRRELDGRLAVRLREELDVRYELGRLDDGGLGLLLRTPDEPEAAASILR
                     RVREAAVDVAAPAAGLGIGVAVYPRDGRTGEALLETASRSLWLFERLTREVWPWPEAS
                     CAAAAPRPRISDGSDGKEENHDFTA"
     gene            2197..2712
                     /locus_tag="VEL74_08405"
     CDS             2197..2712
                     /locus_tag="VEL74_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62591.1"
                     /translation="MISLREDYRVHVHPTTARLFLWNREHLEASFYLRPGATLGGGWE
                     GLGERLNDPGTRFLACQIDGRVELVHLDALAYVEVTGALPEVARREMVGATRLPAALS
                     LRCGRSLSGEFLAILPPGRRRLSDLLNCHDERFLLLVNPAVTRYVHRDAVARVIPADD
                     PDLERDEENLV"
     gene            complement(2709..>3264)
                     /locus_tag="VEL74_08410"
     CDS             complement(2709..>3264)
                     /locus_tag="VEL74_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYG62592.1"
                     /translation="KKSVFSQREVGTDTESFSAALRSAIRQDADVVLVGEMRDLETIS
                     LAVTAAEMGALVFGTLHTNGAANTIDRLIDAFPADEQAQIRTTLAESMAGVVSQLLLR
                     TADGQGRCAVNEILLKTPGLPNVIREGNTPMILSLIQGGKAMGMQLMDDALMALVEEG
                     RITARDAYMKAANKARFENLVEED"
BASE COUNT          454 a         1096 c         1160 g          554 t
ORIGIN      
        1 ctcaacgtct cggccaggct cttctccgat ccgagcctgg cgggacgggt gcagacggcg
       61 ctgcaggagg cccacgccgg cggcgagcgg ctggtgctcg agatcaccga gagcgccctg
      121 atggaggagc ccgagaccgc caccgaggtc ctgtcccgga tccggacgca aggggtccag
      181 gtcgccatcg acgacttcgg caccggctac tcgtccctga gctatctcca gcagttcccg
      241 atcgaaagcc tcaagatcga ccggtctttc atcgccaggg tcggagagca gggtgagaac
      301 gccgagatcc tgcgcgccat tctcaccctg gcccgaagcc tcggcatccg tgtcatcgcc
      361 gagggggtgg agaccggaga gcagctcgcg cagctcgtcg ccatgggctg tgacatggcc
      421 cagggctacc tcttctcgcc gccggtggat gccgggacgg cgcgcgccct gctcgcccag
      481 ccgcccgcct gggcgggcct cctccgccca ttctgtccgc gcccggcgct gaagattgtc
      541 gggcggggct tctgacccct gacttccgag gagacgtcac gatggcatcc gagattctcc
      601 agtggccgtt gacgaccgcg ttcgccgccg agcagcgggc cgcccgcctt ggcaatatcc
      661 tggaggagac caccgacgcc gtgctcatga aggacctcgc gggccgctac cacttcgcca
      721 accgcgccgc cgccctcctc ctgggccgcg ggccgcacga ggtggtcggc cggagcgatg
      781 ccgagttgct cgctgccgac gccgcggccg cggtccgcga cgtcgagcgc cgggtcctcg
      841 acgcggggag gaccctcgtc cacgaggaga cacgcacggt agacggcctg ctccgccagt
      901 gcgtttcgac ccgcggcgtc tttcgcaatg tgcacggcga ggtgaccggc ctcttctgtg
      961 tcacccgcgc cgcaggaggg agccgggagg tcgaggcgga ggtggtctcc ctggccgcgg
     1021 aggtggagac gccgcaggag gcggccctga tcctgcgacc ctttcacctc cacgccgtcg
     1081 ccgccctgct gcgccagcgg cgggaccggc aggcgtcgtg atttttttgt tgcttaaatt
     1141 tagatggtga gctaccctgg tcgctccatc gaggttgcta tcatgcatac agtcaccgag
     1201 gtctggggac gggagtggga ggagcatagt ctgccgttgt tcgagttgct ccagcgggcc
     1261 gagcgtgggg gagtgacgga catctgccag ctcctgcgcc acgtccgtca ctgccggacc
     1321 acgctgaccg aagcgccgac tccccggctc caggggctgg ccgtccgtac cctggacgag
     1381 ctgtgggaag cggcgcggct gaccctgtac ggcgacctgg gcctggccga cgagcatacg
     1441 gccgcggcct ggggccacgc ccagaagatc caccagctcc tgtcggactg ctgaccctcg
     1501 tccgtgtcct ggacgatccg tccagctttc acgctcgccg ttccctccgc agcttgaaaa
     1561 taaaggtttt catgcgggta tcggccttgc aatgatccgc tgtgtccaac ttttcgagcc
     1621 ggcggtccgt ggcggccgtc caggaggaga gcgacatgga tgcagttgca gacccggagt
     1681 gtgcaatcgg cggggggccc ctcgatccac gatcgtttct tgggatcctg tcggggctat
     1741 ccaacgagga tgacggagag agcggggcta ccctgctgct gctgctgctg cgcccccgct
     1801 ggggtgaggc cgcgccgcgg agggagctgg acgggcggct cgccgtccgg ctgcgggaag
     1861 agctggatgt ccggtacgag ctgggccgcc tcgacgacgg cggcctcggg ctcctgctgc
     1921 ggacgccgga cgaacccgag gccgccgctt cgatcttgcg gcgggtgcgc gaggccgccg
     1981 tcgatgtcgc cgcgccagcg gcgggcctgg gcatcggcgt cgcggtctat ccgcgggatg
     2041 gaaggacggg ggaagcgctc ctcgagaccg ccagccgttc gctctggctc ttcgagcggc
     2101 tcacccggga ggtctggccc tggccggagg cttcctgcgc cgcggccgcg ccgcgcccac
     2161 ggatctcaga tggatcagat ggaaaggaag agaatcatga tttcactgcg tgaggactat
     2221 cgcgtccacg tccacccgac caccgcgcgg ctgttcctgt ggaaccgcga gcatttggag
     2281 gcttcgttct atctgcgccc cggcgctaca ttgggcggcg gctgggaagg gctgggggag
     2341 cggctgaatg atccggggac acggttcctc gcctgccaga tcgacggccg ggtcgagctc
     2401 gtccatctgg acgctctcgc ctatgtggag gtgaccggcg ccctgccgga ggtggcacgg
     2461 cgggagatgg tcggcgccac gcgcctgccc gccgcgctgt cgctgcgttg cggccgttcg
     2521 ctgtcgggcg agttcctggc catcctgccg cctggtcggc gccggctgtc cgacctgctg
     2581 aactgccacg acgagcgctt cctgctgctg gtgaacccgg cggtcacgcg ctacgtccac
     2641 cgcgacgccg tcgcgcgcgt cattccggcg gacgatccgg atctggagcg tgacgaggag
     2701 aatctggtct agtcctcttc gaccaggttc tcgaaacggg ccttgttggc cgccttcatg
     2761 taggcgtcgc gggcggtgat gcgcccctcc tcgacgaggg ccatcagggc gtcgtccatg
     2821 agctgcatcc ccatcgcctt gccgccctgg atgagcgaca ggatcatcgg cgtgttgccc
     2881 tcgcggatga cgttgggcag gcccggggtc ttgagcagga tctcgttgac ggcgcagcgc
     2941 ccctggccgt cggcggtgcg cagcaggagc tgcgagacga cgccggccat ggattcggcc
     3001 agggtggtgc ggatctgcgc ctgctcgtcg gccgggaagg cgtcgatcag gcggtcgatg
     3061 gtgttggcgg cgccgttggt gtggagggtg ccgaagacca gggcacccat ctcggcggcg
     3121 gtgacggcga gcgagatggt ctccaggtcc cgcatctcgc cgaccaggac cacgtccgcg
     3181 tcctggcgga tggccgagcg cagcgccgcg ctgaagctct cggtgtccgt cccgacttcg
     3241 cgctgggaga agaccgactt cttg
//
LOCUS       DASPJI010000316         6784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_533534, whole genome shotgun sequence.
ACCESSION   DASPJI010000316 DASPJI010000000
VERSION     DASPJI010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533534"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4447)
                     /locus_tag="VEL74_08415"
     CDS             complement(<1..4447)
                     /locus_tag="VEL74_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HYG62593.1"
                     /translation="MEDLDRIAIIGRSCRFPGAADADRFWELLRDGVEAVTFFSIEEL
                     TAAGLPAELVRRPDYVPAKAIVEGTDLFDADFFGFTPREAELMDPQQRLFLECAWEAC
                     EDAGYDPHGYTGRVGVYGGSGFNGYLTGNLAYRPDLLTAAGGLQTRLFVDKDFLTTHV
                     SYKLNLRGPSMAVQTACSTSLVAVHLACQALLEGECDMALAGGVTLTFPQRTGYLFQE
                     GSIHSPDGHTRAFDERAQGTVAGEGVGVVLLKRLGDALADGDPIYAVILGSAANNDGA
                     AKAGYTAPSIERQVEVALEALTLAQVDPATVGSIEAHGSGTPLGDPIEVEALTRAYRS
                     AEGGRGTCALGSVKTNIGHTDAAAGVASLLKAVESLRHRQIPPSLNFERPNPRMRLED
                     GPFYVNTALRDWPAGSAGVRRAAVHSLAIGGTNVHLVLEEAPPRPSSSAAAPSRPWQL
                     LVLSARTPAALDEATARLARRLAADPGLGGPGLADVAHTLRVGRKAFRHRRVLVCRDR
                     EDAVAALTSADPAFLPTGEAGAEERRVAFLFPGLGDHHAGMAAELYEREPVFRAAIDR
                     CAELLAPGLRELGTGLRELLHTAGLQAAAGRHPAQRQNDLRSLLAAPSPAADAGETSD
                     PLLRTALAHPAVFAFEYALARLWESWGIRPRAMIGYSLGEYVAACLAGVFSLEDALAF
                     VAARARLIDALPAGAMLAVPLPAAEMEPLLAAAPELSLSAANGPALSVVGGTPAAVAD
                     LERRLAERGVSCRRLQTSHAFHTPLMEPVLPEFRRLLTTVGPRLGAPSIPFVSNVTGT
                     WITAEQATDPEYWARHLCRTVRFAQGLETLMTGSPGEPWALLEVGPGQSLATLARQHP
                     GGPAGQVVMASLPDRRDGVSDQAFVLETLGRLWLAGVAPDWQELVRGEQRRRVPLPTY
                     PFERRRYFVDPPAPGSLLSLASTPVRRDLKDWFWTPVWERLPAGAAAPAGEAAARHWL
                     LFTGEGDELGHRLARRLEAGGARVSRVVAGPGLEPFAAAGPGLYVLDPRSPEGYRALF
                     RALAGSPEGLPDGIAHLWSVTGERRLAIDEVQDLGFYSLLWIAQALGELEPRPIRLAA
                     VADGLFEVTGGETLQPEKATLLGPCRVLPQEYPHLTAAAVDIVTGGPLEELAERLATV
                     LTAPLGPAAAAPVIALRGRFLWMQRFRPLRLASDAPAVPRLRRGGACLVTGDLDTTQL
                     TLAEHLFRSAGARLALLVPAGTPPRGKWAEWLSAHDEADSTSRALRRLLTLEAAGCEL
                     LVLPMPAPARIEEAVQKVRQRFGALHGLIHAAGGPGAGLAQWKTHGMAAQVLGPKVQG
                     TLALAAALADEPLDLFVLFGSSTAATGGFGQVDTCAAASFLDAYAQARNAEGASFVQS
                     LGWSNFRWQEVTAADPVLAAQLRAGLEAFGIGAEDLVTAFDRALASPLPQVVVATRDL
                     DSLIAEADTVAAAAFAPGTAGAVAATEATSGFHPRPELGVPYAEPR"
     gene            complement(4503..>6784)
                     /locus_tag="VEL74_08420"
     CDS             complement(4503..>6784)
                     /locus_tag="VEL74_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62594.1"
                     /translation="HQDVPFEKLVEDLEPERSLTFTPFFQVLFTLDRPPLPADLSDLA
                     PATMTPQIGSAKFDLTFTLMELPDGLVGSVEHTTDLFDTTTALRLAGQLETLLAGFVA
                     HPGRAAGELPLLAEAERHQVLIEANEVAGSPPASTLHGLFFRQAERTPDRPALHWQEG
                     TLTYGELRRRVDGLAIHLRRLGVGPEVPAAVFLDRRPELVAGLLAILAAGGAYVPLDP
                     VYPRERLSLTLEDCQAPVVLTRSGLVDRLPPGPETVLLDVPQGVGDGTGPREAATAGN
                     LAYLIYTSGSTGRPKGVAIEHRSAVALLDWAGELFPDADLAGVLASTSICFDLSVFEI
                     FLPLARGGTIVLADSALDLLAMGDETPVTLINTVPSAAAELTRGRSLPAGVRTLNMAG
                     EPIPPALVARLAAEPRLGRVFNLYGPSEATTYSTWERVAVESDGTPRSPAIGRPIAGT
                     SAYVVDRGQRPVPSGVPGELWLGGAGLARGYLGRPELTAAGFVPDPFGGEPGARLYRT
                     GDLVRRLPDGRLDFLGRIDHQVKVRGFRIELGEIETVLARHPQVAGVTVAVREAKGGA
                     PGERRLVAYVVPRDGAGDLGIQELRGFVRQALPEFMVPSDFVALRTLPLTPSGKVDRK
                     ALPEPGALRLGPETVYAEPKSALEQTVATVLREVLGIERVGLNDNFFDLGGHSLRLVR
                     VAAVLEERLGRPVQVLDLFRSPTVALLCAAWATSEAPAAPPVPEASAEELEQRADDRR
                     QGRARRRELRLDRASQDLP"
BASE COUNT         1005 a         2496 c         2468 g          815 t
ORIGIN      
        1 tgcggggctc cgcgtaggga acgcccagct cgggccgggg gtggaagccg gaggtggcct
       61 ccgtcgccgc aacggcgccg gcagtgccgg gcgcgaaggc cgcggcggcc acggtatcgg
      121 cctctgcgat gagggagtcc aggtcacggg tggccaccac cacctgcggc agcgggctgg
      181 ccagcgcgcg gtcgaaggcg gtgacgagat cctccgcgcc gatgccgaaa gcctccagac
      241 ctgcccggag ctgcgccgcc agaaccgggt ccgccgccgt cacctcctgc cagcggaagt
      301 tgctccagcc caggctctgg acgaaggagg ctccttcggc gttccgcgcc tgcgcgtagg
      361 cgtcgaggaa ggaggccgcg gcgcaggtgt ccacctggcc gaagccgccg gtggccgcgg
      421 tgctggagcc gaagagcacg aacaggtcga gcggctcgtc cgccagggcc gcggcgagcg
      481 ccagagtccc ctgtaccttg gggccgagga cctgcgcggc catgccgtgg gtcttccact
      541 gcgccaggcc cgctcccggc ccgcccgccg cgtgaatcag gccatgcaga gcgccgaagc
      601 gctgccgcac cttctgcacc gcctcctcga tccgtgccgg tgccggcatc ggcaacacca
      661 gcagctcgca gccggcggcc tccagcgtca ggagtcgccg aagagcccgc gaggtcgagt
      721 cggcctcgtc gtgggccgac agccactcgg cccacttgcc acgcggtggt gtgccggcgg
      781 gcaccagcag cgccaggcgg gcccccgcag accggaacag atgctccgcc agggtgagct
      841 gggtcgtgtc cagatcgccg gtgaccagac aggcgccgcc ccgccgcagc cgcggcaccg
      901 ccggcgcatc ggacgccagc cgcaacggac ggaaccgctg catccagagg aaacggccgc
      961 gcagcgcgat caccggcgcc gcggccgcgg gccccagcgg cgccgtcagc acagtggcca
     1021 ggcgctccgc cagctcctcc aggggcccgc cggtcacgat gtcgacggcc gcggcggtca
     1081 gatgcgggta ctcctggggg agcacgcggc agggccccag cagcgtcgcc ttttccggct
     1141 ggagcgtctc gccaccagtt acctcgaaga gaccgtcggc gacggccgcc aagcggatgg
     1201 gccggggctc caactcgccc agagcctgcg cgatccagag caagctgtag aagccgaggt
     1261 cctgcacctc gtcgatggcg agacgcctct cgccagtgac gctccacagg tgggcgatgc
     1321 cgtccggcag tccctccggt gagccggcga gagcgcggaa cagggcgcgg taaccctccg
     1381 ggctccgcgg gtcgaggaca tagagccccg ggccggcggc ggcgaacggc tcgagcccgg
     1441 gacccgccac gacgcgcgac acccgcgcgc ctccggcctc caggcgccgg gccagccggt
     1501 gccccaattc gtccccctcg ccggtgaaga gcagccagtg gcgggcagcg gcttcgccag
     1561 ccggcgccgc ggcgccggcc ggcagacgct cccataccgg cgtccagaac cagtccttca
     1621 agtcccggcg aaccggcgtg gacgccaggg atagcagcga cccgggcgcc ggcggatcga
     1681 cgaagtaacg ccgtcgctcg aagggatagg tggggagcgg cacacggcgg cgctgctcgc
     1741 cgcggaccag ctcctgccag tccggcgcca cgcccgccag ccagagccgg cccagcgtct
     1801 ccaggacgaa agcctggtcc gagacgccat cgcgccggtc cggcagcgac gccatcacca
     1861 cctgcccggc cggccccccg ggatgctgac gggcgagcgt cgccagtgac tggccgggtc
     1921 cgacctcgag cagtgcccag ggctcacctg ggctgccggt catcagcgtc tccagcccct
     1981 gggcgaagcg caccgtccgg caaagatgcc gcgcccagta ctccggatcg gtcgcctgct
     2041 ccgccgtgat ccaggtgccg gtgacgttgg agacgaaggg gatgctcgga gctccgaggc
     2101 gggggcccac cgtggtgagc aggcgcctga actcggggag aaccggctcc atcagcgggg
     2161 tatgaaaggc gtgcgacgtc tgcagccgcc ggcaggatac gccgcgctcc gccagccgcc
     2221 gctccagatc cgccaccgcc gccggcgtgc cgcccactac cgacagcgcc ggcccgttgg
     2281 cggccgacag cgacagctcc ggcgcggcgg cgaggagtgg ctccatttcg gcggccggca
     2341 gcggcacggc cagcatggcc ccggctggca gcgcgtcgat caggcgagcg cgcgcggcca
     2401 cgaacgccag ggcatcctcg agagagaaga cgccggcgag gcaggccgcc acatactcgc
     2461 cgaggctgta gccgatcatc gcccgcggcc ggattcccca tgactcccac agcctcgcca
     2521 gcgcgtactc aaaggcgaag accgccggat gggcaagggc ggtgcgcagc agagggtccg
     2581 acgtctcgcc ggcgtccgca gccggcgacg gcgccgccag caggctgcgc aggtcattct
     2641 ggcgctgtgc tggatgccgg ccggcggcgg cctggagccc ggcggtatgg agcagctcgc
     2701 gcagcccggt gcccagctcc cgcaggccgg gggccagcag ctcggcacaa cggtcgatgg
     2761 cggcgcggaa gaccggctcc cgctcgtaca gctcggccgc catgcccgca tggtgatcgc
     2821 ccaggcccgg gaagaggaag gcgacgcggc gctcctccgc gccggcctcg ccggtgggga
     2881 ggaaggccgg atcggcgctc gtgagagccg ccacggcgtc ttcgcggtca cggcagacca
     2941 gaacacggcg gtgccggaag gccttgcggc cgacgcgcag ggtatgcgcc acgtccgcca
     3001 aacccggtcc gccgaggccc gggtccgccg ccagacggcg ggcgagccga gccgtcgcct
     3061 cgtcgagcgc cgccggcgtc cgcgccgaca acaccaggag ctgccagggg cgcgacggtg
     3121 cggccgccga cgaagacggc cgtggcggcg cctcctcgag taccagatgc acattggtgc
     3181 cgccgatcgc cagcgagtgg accgcggccc tgcgcacccc ggcggagccg gccggccagt
     3241 cgcgcagcgc cgtgttgacg tagaacggac cgtcctccag ccgcatgcgc gggttgggcc
     3301 gctcgaagtt gaggctgggc ggaatctgcc tgtggcgcag ggactccact gccttgagga
     3361 ggctggcgac ccctgccgcc gcgtcggtgt gcccgatgtt ggtcttgacc gagccgaggg
     3421 cgcacgtgcc ccgccctccc tccgccgacc ggtaggcgcg ggtgagggcc tcgacctcga
     3481 tcggatcgcc caatggcgtg ccgctgccat gggcctcgat gctgccgacg gtcgccgggt
     3541 cgacctgggc cagggtgagc gcctccagcg ccacctcgac ctggcgctcg atgctggggg
     3601 ccgtgtagcc cgccttggcc gcgccgtcgt tgttggccgc cgagcccagg atgacggcgt
     3661 agatcggatc gccatcggcc agggcgtcac ccaggcgctt gagcagcacc acacccaccc
     3721 cctcgccggc caccgtgccc tgcgcgcgct cgtcaaaggc gcgggtgtgg ccatccggcg
     3781 agtggatgct cccctcctgg aagaggtaac cggtgcgctg cgggaaggtc agggtgacgc
     3841 cgccggcgag cgccatgtcg cactccccct cgagcaacgc ctggcaggcg agatggaccg
     3901 ccaccagcga tgtcgagcag gcggtctgca ccgccatgct gggaccgcga aggttcagct
     3961 tgtacgagac gtgggtggtc aggaagtcct tgtcgacgaa gaggcgcgtc tgcaggccgc
     4021 cggccgccgt cagcaggtcg gggcggtagg cgaggttccc cgtgaggtag ccgttgaacc
     4081 ccgagccgcc gtagacaccg acccgcccgg tgtagccgtg cgggtcgtag ccggcgtcct
     4141 cgcaggcctc ccaggcgcac tccaggaaga ggcgctgctg cggatccatc agctcggcct
     4201 cgcgaggggt gaagccgaag aagtcagcgt cgaagaggtc cgtcccctcg acgatcgcct
     4261 tggccggaac atagtcgggc cggcgcacca gctcggcggg gaggccggcc gcggtcagct
     4321 cctcgatgct gaagaaggtg accgcctcga caccgtcgcg cagcagctcc cagaaccggt
     4381 cggcgtccgc cgctccgggg aagcggcagg agcggccgat gatggcgatc cggtcgaggt
     4441 cttccatacg gtctctatcc attccaacgg gtcctctcgt cttcggcgta gcggtggcgg
     4501 cctcacggca ggtcttggct ggcgcggtcg agccgcagct cgcggcggcg cgcccgccct
     4561 tggcggcgat cgtcggcgcg ctgctccagc tcctcggcgc tggcctcggg gaccggaggg
     4621 gccgcggggg cctcgctcgt agcccaggcc gcgcagagca gcgccaccgt cggcgagcgg
     4681 aacaggtcga gcacctgcac cggccgaccg agacgctcct cgagcaccgc cgcgacccgc
     4741 accaggcgca gcgagtgacc gcccaggtcg aagaagttgt cgttgaggcc gacgcgctcg
     4801 atgcccagca cctcacgcag caccgtggcg accgtctgct cgagggcgct cttcggttcg
     4861 gcgtagaccg tctccggccc gaggcgcaac gcgcccggct cgggcagggc cttgcggtcg
     4921 accttgccgc tgggggtgag cggcagcgtg cggagcgcca cgaagtccga cggcaccatg
     4981 aactcgggga gcgcctggcg gacgaaccct cgcagctcct gaatccctag atcccctgcc
     5041 ccgtcccgcg gcaccacata ggcgacgagg cgccgctccc cgggcgcgcc ccccttcgcc
     5101 tcgcgcaccg cgacggtgac cccggccacc tgcgggtgac gcgccagtac cgtctcgatc
     5161 tcccccagct cgatccggaa gccgcgcacc ttgacctggt ggtcgatgcg cccgaggaag
     5221 tcgagccggc cgtcgggcag ccggcgcacc agatcccctg tccggtagag gcgcgccccc
     5281 ggctcaccgc cgaaaggatc gggaacgaaa cccgccgccg tcagctccgg ccggccgaga
     5341 tagccgcggg cgagaccggc gccgccgagc cagagctccc ccgggacccc agacgggacg
     5401 gggcgctgcc cgcgatcgac cacgtacgcc gaggtgccgg cgatcggccg tccgatggcg
     5461 ggcgaccggg gtgtgccgtc gctctcgacc gccacccgct cccaggtgga ataggtggtg
     5521 gcttccgacg gaccgtagag gttgaagacc cggccgagcc ggggctccgc agccagccgc
     5581 gccacgaggg ccggtgggat cggctcgccc gccatattga gcgtccgcac gccggccggg
     5641 agcgagcgcc cgcgggtcag ctccgccgcc gccgacggca cggtgttgat cagcgtcacc
     5701 ggcgtctcgt cgcccatggc cagcagatcg agagcgctgt cggccagcac gatggtgccg
     5761 ccgcgggcga gcggcaggaa gatctcgaag accgacaggt cgaagcagat cgaggtggag
     5821 gcgagcacac cggcaaggtc ggcgtcgggg aagagctcgc ccgcccaatc gagcagcgcc
     5881 acggcgctgc ggtgctcgat cgccaccccc ttcggccggc ccgtcgagcc ggaggtatag
     5941 atgaggtagg cgaggttgcc ggcggtggcg gcctctcgtg ggcctgtccc gtcgcctaca
     6001 ccctggggaa catccagcag caccgtctcc gggcctgggg gcagccggtc caccagtccg
     6061 ctgcgggtga ggaccaccgg cgcctggcag tcttcgagcg tcagcgacag gcgctcgcgc
     6121 ggatagaccg ggtccagcgg cacgtaggcg ccgccggccg cgaggatcgc cagaagaccg
     6181 gccaccaact ccggccgccg atcgaggaag accgccgccg gcacctccgg ccccacgccc
     6241 aaccgccgca ggtggatcgc caggccgtcc acccgccgcc gcagctcgcc gtaggtcaac
     6301 gttccctcct gccaatgcaa ggccggccgg tcgggagtgc gctccgcctg acggaagaag
     6361 aggccgtgga gcgtcgaggc cggcggcgac ccggccacct cgttggcctc gatcagcacc
     6421 tgatgccgct cggcctccgc gagcagcggc agctctccgg ccgcccttcc cggatgagcg
     6481 acgaagccgg cgagcagcgt ctcgagctgg ccggcgaggc gtagcgcggt ggtggtgtcg
     6541 aacaggtcgg tggtgtgctc caccgatccg acgaggccgt cgggcagctc catcagcgtg
     6601 aacgtgaggt cgaacttggc gctgccgatc tgcggcgtca tcgtcgccgg cgccaagtcg
     6661 ctcaggtcgg ccggcagcgg cggtcggtcg agggtgaaca gtacctggaa gaacggtgtg
     6721 aaggtgaggc tgcgctccgg ctccaggtcc tccaccagct tctcgaacgg cacgtcctgg
     6781 tgcg
//
LOCUS       DASPJI010000317         4231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_78461, whole genome shotgun sequence.
ACCESSION   DASPJI010000317 DASPJI010000000
VERSION     DASPJI010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78461"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VEL74_08425"
     CDS             <1..1073
                     /locus_tag="VEL74_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62595.1"
                     /translation="GYALGGLFLGPLLFTPFGAGGPEPTLQSHVRNAPFRFFPMELSL
                     RNVPGYERFVMGGLRIIGRKDLFLPHGGPLWVRGASRTELYVSSAEPIERMVFLVQSI
                     APNNRVTLDSGSNREVLSMKRGEEQRVELPLERPFRVRIVGGRKLYYYRLVVTTSTGR
                     VRHWTRFYPPNSCPYFAENDATRENFFVGAVLTYMGSGKHLNADLYTIQWGKVVAPRQ
                     VRAGEIFTVATRLFNRSDVPWTADGAARVNLSYHWITGGRMVIQDGERTLLPLPVPPG
                     GRVSVRQRIKAPPEPGWYVLELDPVFENVAWFSDANGGNTYRVQVQVLPALPAAAPPT
                     VAPAAPTTPSEDAEEEETGDAR"
     gene            1063..1605
                     /locus_tag="VEL74_08430"
     CDS             1063..1605
                     /locus_tag="VEL74_08430"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HYG62596.1"
                     /translation="MLDNLRQDSLRLLEIKSKAFPWYVIESLLFENGYQAVVLHRIAH
                     WFKRRGIPFFGPLFARLNLLLTGVDIGPGAEIGPGLRIAHGTGLVIGGYARIGAGALL
                     LHGVTIGSPHPGRVEAMPRIGDNVFIGAGAKVIGDITVGDDVFIGVNAIVARDIPSGS
                     RVLSTAEIDVAPRKDLDGAL"
     gene            complement(1602..2045)
                     /locus_tag="VEL74_08435"
     CDS             complement(1602..2045)
                     /locus_tag="VEL74_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62597.1"
                     /translation="MEIHPLARELASFLTPLLPHLARLGEEDAELAGAQLAGDRWEDA
                     KALWRRLRPQMMEHQAAAAAGEVAARPESPAAGDALAAQIDGLLRNDPDLASQVELRF
                     LDPTRVTSGDVVVTANNTHDALAVAAGPGVSEEEVAVIAPGDRAR"
     gene            complement(2061..2495)
                     /locus_tag="VEL74_08440"
     CDS             complement(2061..2495)
                     /locus_tag="VEL74_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62598.1"
                     /translation="MNISPLAEALTSFLVPLLPHLVRMGEEESELAGEKLAGARWQDA
                     RRLWRRLGPLVAETPETVRAGEAAVTLPENPEVQDTLATRLEALLESDPELAGELERV
                     LDPGRVTSGYEVVPGGNVSEGTVVGDSDHAVTISLSGDENEI"
     gene            complement(2578..3699)
                     /locus_tag="VEL74_08445"
     CDS             complement(2578..3699)
                     /locus_tag="VEL74_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HYG62599.1"
                     /translation="MGTQPRVAIVGGGPAGAVAALVLARRGVPVVVLEAHAAPALKVG
                     ETLPPGILPLLQHLDLQRRLESDGHLRSQGNRSVWGSDEPGERSFLADPYGEGWHVDR
                     RRFEARLAEAAREAGAEWRWGCRLESCDAMDGGWRLAIAGEAPERLDVELVADATGRP
                     ARLARRLGARRLRYDRLVGIAGLLASASPAADTYTLVEATPEGWWYSALLADGRLAVA
                     FLGDGDLLERPLLNARESSSAWWERLAQTAATRERVEAHGYHLNSPLRPLPAESSRLD
                     VIAGEGWLALGDAAAAYDPLSSHGIASAMGSGFYGGHALADLLEGRDEAPLAYLHVMQ
                     QAYGAYLDLLREHYAAERRWPDAPFWRRRHSPGFCLEPF"
     gene            complement(3702..>4231)
                     /locus_tag="VEL74_08450"
     CDS             complement(3702..>4231)
                     /locus_tag="VEL74_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007093693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62600.1"
                     /translation="GIPWQTDEASCLAGYDASAYLPLPSFWAARVPNQVLSHKAFERT
                     TDSGLPAVQRLKHFGYRQFWLRDLQGSGYLQRIDNMVKYWHMVGIVAEQPAPVGTGEE
                     GLPDRYWVETQRDPSFSRSDATWKQLLMVERAEPMSLRTKLAAKSSAALAAELDMEPE
                     EVVHPVRRNVQRDQR"
BASE COUNT          604 a         1565 c         1458 g          604 t
ORIGIN      
        1 tgggctatgc cctgggcggg ctcttcctcg ggccgctcct cttcaccccg ttcggcgccg
       61 gcggccccga gccgaccctc cagtcgcacg tacgcaacgc gccgttccgc ttcttcccga
      121 tggagctgtc gctccgcaac gtccccggct acgagcggtt cgtcatggga ggcctgcgga
      181 tcatcgggcg caaggatctc ttcctccccc acggcggccc cctctgggtg cggggcgcca
      241 gccggacgga gctctacgtc tccagcgccg agccgatcga gcggatggtc ttcctggtgc
      301 agagcatcgc ccccaacaac cgggtgacgc tggactcggg gagcaaccgc gaggtcctgt
      361 ccatgaagcg cggcgaggag cagcgggtag agctccctct ggagcgaccg ttccgcgtgc
      421 gcatcgtcgg cggacgcaag ctctactact accgtctggt ggtgaccacc agcaccggcc
      481 gcgtccgcca ctggacccgc ttctatcccc cgaactcctg cccctacttc gccgagaacg
      541 acgcgacgcg ggagaacttc ttcgtcggcg ccgtgctcac ctacatgggg agcggcaagc
      601 acctgaacgc cgacctctac accatccagt ggggcaaggt cgtggcgccg cggcaggtgc
      661 gggccggcga gatctttacc gtcgccaccc gcctgttcaa ccgcagcgac gtcccctgga
      721 cggctgacgg ggccgcgcgg gtgaacctct cctatcactg gattaccggc ggccggatgg
      781 tcatccagga cggcgagcgg acgctcctcc ccctgccggt gccgccgggc ggccgggtga
      841 gcgtccggca gcggatcaag gcgccgccgg agccgggctg gtacgtgctg gagctcgatc
      901 cggtgttcga gaacgtggcc tggttctcgg acgcgaacgg cggcaacacc taccgcgtcc
      961 aggtccaggt tctgccggca ttgccggcgg ccgccccgcc cacggtggct ccagccgctc
     1021 cgaccacccc cagcgaggac gcggaggaag aggagacggg ggatgctcga taacctgcgc
     1081 caggacagcc tccggctcct ggagatcaag agcaaggcgt tcccctggta cgtgatcgag
     1141 tcgctgctgt tcgagaacgg ttaccaggcc gtggtgctgc accgcatcgc ccactggttc
     1201 aagcggcgcg gcatcccctt cttcgggccg ctgttcgccc ggctcaacct cctcctgacg
     1261 ggggtggaca tcggcccggg cgccgagatc ggtccggggc tgcgcatcgc ccacggcacg
     1321 gggctggtga tcggcggcta cgcccggatc ggcgccgggg ccctcctcct ccacggcgtc
     1381 accatcggct cgccgcatcc cggccgggtg gaggccatgc cccggatcgg tgacaacgta
     1441 ttcatcggcg cgggcgccaa ggtgatcggc gacatcaccg tcggcgacga cgtcttcatc
     1501 ggcgtcaacg ccatcgtcgc ccgggacatc ccgagcggca gccgggtgct cagcacggcc
     1561 gagatcgacg tcgcgccgcg caaggacctg gacggggccc tctagcgcgc ccggtccccc
     1621 ggcgcgatga ccgccacctc ttcctcggac acgccgggac ccgcggccac cgccagcgcg
     1681 tcgtgggtgt tgttggcggt gacgaccacg tcgccggacg tgacccgggt gggatcgagg
     1741 aaccgcagct ccacctggct ggcgaggtcc ggatcgttcc ggagcaggcc gtcgatctgg
     1801 gccgccaggg cgtctcccgc ggccggagac tccggccggg cggcgacctc accggcggcc
     1861 gcggcggcct ggtgctccat catctgcggg cgcagccggc gccacagggc cttcgcgtct
     1921 tcccagcggt ctcccgcgag ttgggctccg gcgagctcgg cgtcctcctc ccccagcctc
     1981 gccagatgcg gcagcagggg tgtcaggaag ctcgcgagct cacgggccag ggggtgaatt
     2041 tccataccgt ctccttcttc ttagatctcg ttctcatcgc ccgagaggga gatggtcacg
     2101 gcgtgatcgc tgtcgcccac caccgtcccc tcgctgacgt tgccaccggg aaccacctcg
     2161 tagccgctgg tgacccggcc cggatcgagg acccgctcga gctcgccggc gagctccgga
     2221 tcgctctcca ggagggcctc gaggcgcgtc gccagggtgt cctgcacctc ggggttctcg
     2281 ggcagggtca cggccgcctc gccggcccgg acggtctccg gggtctcggc cacgagcggc
     2341 cccagccggc gccagagcct tctggcgtcc tgccagcgcg cccccgccag cttctccccc
     2401 gccagctcgc tctcctcctc gcccatcctg accagatgcg gcagcaaggg gaccaggaag
     2461 ctcgtcagcg cctccgccag cggtgagatg ttcatggccg tctccttccc ggacagcctg
     2521 cccgggctca gcgggaggag ggttgcaagt gacggtccag gaatccccga gcccgcttca
     2581 gaagggctcc agacagaacc cgggcgagtg ccggcggcgc cagaaggggg cgtccggcca
     2641 gcgccgctcc gccgcataat gctcgcgcag cagatccaga taagcgccat aggcctgctg
     2701 catcacgtgg aggtaggcga gaggggcctc atcccggccc tccagcaggt cggccagggc
     2761 gtggccgccg tagaagcccg accccatggc ggacgcgatg ccgtgggacg agagcgggtc
     2821 gtaggccgcc gcggcgtcac ccaaggccag ccagccctcg cccgcgatga cgtcgaggcg
     2881 ggagctctcg gccggcagag gccgtagcgg cgaattcagg tggtacccgt gggcctcgac
     2941 ccgctcccgg gtggccgcgg tctgagccag gcgttcccac caggcagacg agctctccct
     3001 agcgttcagg aggggacgct cgaggagatc gccatcgccc aggaaagcca cggccagccg
     3061 gccgtccgcc agcagggcgg agtaccacca gccctccggg gtggcctcga ccagggtata
     3121 ggtgtccgcc gccgggctgg cgctggcgag gaggccggca atcccgacca ggcggtcata
     3181 gcgcagccgc cgcgctccga gccgccgggc caggcgggcg ggacgccccg tcgcgtcggc
     3241 caccagctcg acgtccaggc gctctggagc ttcgccggcg atggcgagcc gccagccgcc
     3301 gtccatggcg tcgcagctct cgagccggca gccccacctc cattcggccc cggcctcccg
     3361 cgccgcctcg gcgagccgcg cctcgaaccg ccggcggtcc acgtgccagc cttcgccgta
     3421 cggatcggcc aggaaagagc gctcgcccgg ctcgtcggag ccccagacag accggttccc
     3481 ctgcgagcgg agatggccgt cggactccag ccggcgctgg agatcgagat gctgcaggag
     3541 ggggagaatg cccggcggca aggtctcccc caccttgagg gcgggcgcgg cgtgcgcctc
     3601 gaggaccacc accgggacgc cgcgccgcgc caggacgaga gcggcgaccg cgccggcagg
     3661 accgccgccc acgatcgcca ctctcggctg agtgcccatc ctcagcgctg atcccgctgg
     3721 acgttgcggc gcaccggatg caccacctcc tccggctcca tatcgagctc cgcggcgagg
     3781 gcggcgctgg acttggccgc cagcttcgtc cgcaggctca tgggctccgc ccgctccacc
     3841 atgaggagct gcttccaggt ggcgtcggaa cggctgaacg acgggtcgcg ctgggtctcg
     3901 acccagtagc ggtccggcag cccctcttca ccggtcccca cgggcgccgg ctgctcggcg
     3961 acgatgccca ccatatgcca gtacttgacc atgttgtcga tacgctggag gtaccccgag
     4021 ccctggaggt cgcgcagcca gaactgccgg tagccgaagt gcttgagccg ctggacggcg
     4081 ggcaggccgg agtccgtggt ccgctcgaag gctttgtggc tgagcacctg attgggtacg
     4141 cgcgccgccc agaacgacgg cagggggaga taagcggagg cgtcgtagcc tgccaggcag
     4201 ctcgcctcgt ccgtctgcca ggggatgccc a
//
LOCUS       DASPJI010000318         4836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_590795, whole genome shotgun sequence.
ACCESSION   DASPJI010000318 DASPJI010000000
VERSION     DASPJI010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590795"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VEL74_08455"
     CDS             <1..285
                     /locus_tag="VEL74_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYG62601.1"
                     /translation="SARAERWIGDIENPKFARRLEVVEQLIPMAEAKEVPLARFANAW
                     ALRHPAVSSVIIGPRTIEQLEDSLLALEVEISEEEARRIDELVPPGTSAL"
     gene            complement(287..877)
                     /locus_tag="VEL74_08460"
     CDS             complement(287..877)
                     /locus_tag="VEL74_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HYG62602.1"
                     /translation="MIGTWVSSGLVWLLSVTPGGAALTPALIPPPQAGQPETSIVQVV
                     PTGLRSPVQMAMSFLGVPYRMGGSSRKGIDCAGLVRAAYDGLGLTLPRTAAGQFQQGV
                     EISLGELQPGDLIFFRDTYKRGISHVGIYIGEGKFVHAASTKRGVVVTSLSRPYYLSR
                     FAGARRVVETAMAGAVQAGTSAATMVPAEGAALVAR"
     gene            complement(1457..3910)
                     /locus_tag="VEL74_08465"
     CDS             complement(1457..3910)
                     /locus_tag="VEL74_08465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG62603.1"
                     /translation="MEHLSQDLRFAVRTLAKKPSFTLVAVLSLALGIGANTTIFSLVN
                     ALLLRPLPVEEPDRLVAIYNKDVKNPGLGQLSHLNWKDYREQNRVFSGVLGYDWTPMS
                     VATGRGEAELAFGQLVSGNYFDLLGIEPALGRTFLPEEDGAPGAHPVAVVSHRFWEER
                     LGADPGAVGKTIRVNGSSYTVIGVAPATFTGLQVGIRPELWIPMAMNRQIKPDDSINW
                     YDERRGLFVNAVGRLAPGMSLEQVRADLAGIAQRLERDFPDDNRGRGIDLVPLPLATV
                     DPDTRQGVVAASTLLMVVVGLVLLIACANVANLLLARAASRRKEIAIRLSQGAARSRV
                     LRQLMTESLLLALLGGAVGLLLMVWARRALLAFLPSLPFPVAVTLDLGLDPLVLVFTV
                     AVSLLTGLLFGLVPALQTSRPELVSALKNEAAPPAEGRRGFNLRNLLVAGQMALSLLS
                     LIAAGLFLRSLGEAREIDPGFDPSRLAVVSFDAGLQGWDAPRTEQFLRDLTARVAAIP
                     GVESAALAQAGPFQGTFLRSVFLEGGGQEDGTLIPVNLVGTGYFSTMGVPLVRGRLFD
                     ERDREGAVPVVVINQTMAERLWPGKDAVGQRFKFFGDAFSVEVAGVVRDIKYGNLGED
                     PQPYIYQPLEQRSTTGLTLVTRAERDPSAVLPAIQREVRAMAPELPLVNVSTVTKLLD
                     DSLWAPRAGATLLALFGALALALAALGLYGVMSYSVTLRSREIGIRMALGARQLDVLG
                     LVLGQGMKVVAAGGLLGLLLAFFATRLAAGMLVGVSPTDPVAFGTTSALLGLVAMAAI
                     LLPARRATELDPIVVLRYE"
     gene            4004..>4836
                     /locus_tag="VEL74_08470"
     CDS             4004..>4836
                     /locus_tag="VEL74_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HYG62604.1"
                     /translation="MGDLRGRTLFITGASRGIGKAIALRAAVDGANIVIAAKTAEENP
                     KLPGTIHSAAADVEAAGGKALPLQVDIRDEEQVRQAVERTVETFGGIDVLVNNASAIN
                     LTPTLQTPMKRFDLMWSVNARGTFACSQACIPYLEKSSNPHILTLSPPLDLDPKWFAP
                     HVAYTIAKYGMSLCVLGLAGELRGRGIAVNALWPRTTIATSAVKNLLGGEEIVQASRT
                     PEILADAAYAVFRKPSRSFSGHFLIDDVFLSEEGVTDFEKYRVDPARPLMPDFFVPDD
                     VP"
BASE COUNT          838 a         1709 c         1642 g          647 t
ORIGIN      
        1 agcgcgcggg ccgagcgctg gatcggcgac atcgagaacc ccaagttcgc ccggcggctg
       61 gaggtggtcg agcagctcat ccccatggcc gaggccaagg aggtccccct ggcccgcttc
      121 gccaatgcct gggccctccg ccaccccgcg gtcagctcgg tcatcattgg accgcggacc
      181 atcgagcagt tggaggacag cttgctggcc ttggaggtgg agatcagcga agaggaggcc
      241 cggcggatcg acgagttggt gccgccggga acctccgccc tctgaatcag cgcgcgacga
      301 gcgcggcgcc ctcggccggg accatcgtcg cggccgaggt gccggcctgg acggcaccgg
      361 ccatggcggt ctcgaccaca cggcgggcgc cggcgaagcg gctcaggtag tagggccggg
      421 agaggctcgt caccaccacg ccccgcttcg tggaggcggc gtggacgaat ttgccctcac
      481 cgatgtagat accgacgtgg gagatgcccc gcttgtaggt gtcacggaag aagatcaggt
      541 cgccgggctg cagctcgccg agggagatct ccaccccctg ctggaactgc cccgccgccg
      601 tccgcggcag ggtgagcccg agcccgtcgt aggccgcccg caccaggccg gcgcagtcga
      661 tgcccttgcg gctcgagccg cccatacggt aggggacccc caggaagctc atcgccatct
      721 ggaccgggct gcgcagcccg gtgggaacga cctggacgat cgatgtctcc ggttgcccgg
      781 cttgaggagg agggatcagg gcaggggtga gagccgcgcc gccaggcgtg acagagagga
      841 gccagaccag accgctggaa acccaggtgc cgatcacgat gtccacctcc ccagcacgat
      901 gccgctgcct tgcctcaaaa agtcgttgaa cacgagcata ctcgtcctcc ttccggtact
      961 catccatcag tacaagccct gttcactccg gccggacagg ccggggtatg ggcgtcgaag
     1021 aacagcgcgc cggatgacgc gaagagctag gctcgggacg gaggtgcccg tccgccggtc
     1081 agcgactggg cctcgaagcg cgggagagct ggctggggag aaaaagaggc cgcgacaaga
     1141 gctggacggt acggccgcac ttcaggctcg aaacctgcat cctcggtacg ctggagatgc
     1201 aggcacccgg ggcaggcatc ggtgtccgtc cgggcctctt cgacgacaga gaccgagacg
     1261 gaggccgccc ggggcgcgcc gccggagtgt ccggcatggg cgtaggcccc caggagcatg
     1321 ccagccagcg caaagcccag gctcaaagcc gacaggagtc tgcgccggct cgttccaccg
     1381 acgtgggttt cgagcacgca cgcttagata gcacaccgtc tctaaggtgg tcaacccttc
     1441 aggagcgatc tgtctctcat tcgtaccgca gcaccacgat ggggtccagc tccgtcgccc
     1501 gccgcgccgg cagcaggatc gccgccattg ccaccaagcc caacagcgcc gaagtcgtgc
     1561 cgaaggccac cggatcggtg gggctgacgc cgaccagcat gccggccgcg agccgtgtgg
     1621 cgaagaaggc caggaggagc ccgagcagtc cgcccgcggc caccaccttc atcccctgcc
     1681 ccagcaccag gccgagcacg tcgagctgac gcgcgccgag cgccatgcgg atcccgatct
     1741 cccgcgagcg cagggtgacc gagtaggaca tcaccccata cagacccagg gccgccagcg
     1801 ccagggccag ggcgccgaaa agcgccagga gcgtcgcacc ggcccgcggc gcccacaagg
     1861 agtcgtccag gagcttcgtg acggtggaga cgttcaccag cggcagctcc ggcgccatcg
     1921 cccggacctc cctctggatg gcggggagaa ctgctgacgg atcgcgctcc gcccgggtca
     1981 ccagcgtcag gccggtcgtg gagcgctgct ccagcggctg gtagatataa ggttgggggt
     2041 cctcccccag gttgccgtac ttgatgtcgc gcactacgcc ggccacctcg acggagaacg
     2101 cgtcgccgaa gaacttgaag cgctgcccca ccgcgtcctt ccccggccag agcctctccg
     2161 ccatggtctg attgatcacc accaccggca cggcgccctc gcggtcccgc tcgtcgaaca
     2221 ggcggccccg caccagcggc acgcccatcg tcgagaagta gccggtcccc accaggttca
     2281 ccgggatcaa ggtgccgtcc tcctgtcctc ccccttcgag gaagaccgag cgcaggaagg
     2341 tgccttggaa cggcccggcc tgggccagcg ccgcggactc gacgccgggg atcgccgcca
     2401 cccgcgctgt cagatcgcgc aggaactgct cggtccgcgg cgcgtcccat ccctggagcc
     2461 cggcgtcgaa ggagacgacc gccagccgcg aggggtcgaa ccccggatcg atctcgcggg
     2521 cctctccgag gctgcgcagg aacagacccg cggcgatgag cgacagcagc gacaacgcca
     2581 tctgaccggc gaccaggagg ttccgcaggt tgaaaccacg gcgcccctcg gccggcggcg
     2641 ccgcctcgtt cttgagcgcc gagaccagct ccggccgcga ggtctggagc gccggcacca
     2701 gcccgaacag cagcccggtg aggagcgaca cggccacggt gaagacgagg accagcggat
     2761 cgagccccag gtccagcgtc accgcgaccg ggaacggcag gctggggagg aaggcgagga
     2821 gtgcccggcg ggcccagacc atgagcagca ggccgacggc gccgccgagc agcgcgagca
     2881 gcaggctctc ggtcatgagc tggcggagca cccggctccg cgccgccccc tgggagaggc
     2941 ggatggcgat ctccttgcgg cgcgacgcgg cccgggccag cagcaggttg gcgacattgg
     3001 cgcaggcgat gagcagcacc agccccacca ccaccatgag cagcgtggac gccgcgacca
     3061 ccccctgccg ggtatccggg tccaccgtgg ccagcggcag cgggaccagg tcgatccccc
     3121 ggccccggtt gtcatcgggg aagtcgcgct ccagccgctg ggcgatcccc gcgaggtccg
     3181 cccggacctg ctccagcgac atccccggcg ccagccggcc gacggcgttg acgaacagcc
     3241 cccggcgctc gtcgtaccag ttgatggagt cgtccggctt gatctgccgg ttcatcgcca
     3301 tagggatcca cagctccggc cggatcccca cctgaaggcc ggtgaacgtg gccggggcga
     3361 cgccgatcac ggtatacgag ctgccgttga cccggatggt cttgcccacg gcgccggggt
     3421 cggcgccgag ccgctcctcc cagaagcgat gggagaccac cgcgaccgga tgagcgccgg
     3481 gcgccccgtc ctcctccggc aggaaggtcc tgccgagcgc cggctcgatc cccaggaggt
     3541 cgaagtagtt gcccgagacg agctggccga acgccagctc cgcctcgccg cgtccggtcg
     3601 ccacgctcat cggcgtccag tcgtacccca ggacgccgga gaagacccgg ttctgctccc
     3661 ggtagtcctt ccagttgagg tgcgacagct gcccgaggcc ggggttcttg acatccttgt
     3721 tatagattgc caccagccgg tccggctcct ccaccggcag cggccgcagc agcagggcgt
     3781 tgaccaggct gaagatggtg gtgttggcgc cgatccccag ggcgagcgag agcaccgcca
     3841 ccagggtgaa ggacggcttc ttggccaacg tgcggacggc aaagcgcaga tcctggctca
     3901 agtgctccat ggggaggcct cctcagctct cgctatccta cggtggaacc gcgatcccgg
     3961 tttcggtcgc gataggatgt tcctcgaaac gggagggaga gccatgggcg acttgagagg
     4021 caggaccctg ttcatcaccg gcgccagccg gggcatcggc aaggcgatcg cgctgcgggc
     4081 cgcggtcgac ggcgccaaca tcgtcatcgc ggccaagacc gccgaggaga acccgaagct
     4141 gccgggcacg atccactcgg cggcggccga cgtggaggcg gccgggggca aggccctccc
     4201 tcttcaggtg gacatccgcg acgaggagca ggtccggcaa gccgtagagc ggaccgtcga
     4261 gaccttcggc ggcatcgacg ttctggtcaa caacgccagc gccatcaatc tcaccccgac
     4321 gctccagacg ccgatgaaac ggttcgatct catgtggtcg gtcaacgccc ggggcacctt
     4381 cgcctgctcc caggcctgca tcccgtatct cgagaagtcg tccaacccgc acatcctgac
     4441 gctgtcgccg ccgctcgacc tggacccgaa atggttcgcg ccgcacgtcg cctacaccat
     4501 cgccaagtac ggcatgagcc tgtgcgtgct ggggctcgcg ggcgagctgc gggggagggg
     4561 aatcgccgtc aacgcgctgt ggccgcgcac caccatcgcg acgagcgccg tgaagaacct
     4621 tctgggcggc gaggagatcg tgcaggcgag ccgcacgccg gagatcctgg ccgacgcggc
     4681 ctatgcggtc ttccgcaaac cctctcgaag cttctccggc cacttcctca tcgacgatgt
     4741 cttcctgagc gaggagggcg tgaccgattt cgagaaatac cgggtcgacc ccgccaggcc
     4801 actgatgccg gacttcttcg tgcccgacga tgttcc
//
LOCUS       DASPJI010000319         3322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_419769, whole genome shotgun sequence.
ACCESSION   DASPJI010000319 DASPJI010000000
VERSION     DASPJI010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419769"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VEL74_08475"
     CDS             complement(<1..764)
                     /locus_tag="VEL74_08475"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HYG62605.1"
                     /translation="MAGRHPGRQDPSPTLPALPPLGAHVSVAGGLCSAFPRAAELECT
                     AMQVFVKNASQWRGKDLADEEAESFRAAHGASAVGPLVAHASYLINLAATDPLVLERS
                     EAALADELVRCARLGVQGLVLHPGAHMGKGEEAGIDSFVAALDRVLAAVPAEMPGIAT
                     RVLLENTAGQGTCLGCRLEHLERIRSLVSAPHRVGVCLDTCHAFAAGYPIHEAAGREE
                     FLAEVEERLGLDVLGCFHVNDSVKPFASRRDRHAHIG"
     gene            805..1587
                     /locus_tag="VEL74_08480"
     CDS             805..1587
                     /locus_tag="VEL74_08480"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HYG62606.1"
                     /translation="MLSRLLRGLLYLVYGLVVVTTFALAAYVSFSLFVRSGTTRVPPV
                     REMARAEATALLADQGLRIRAAKEGRYDDKVPVGRVVRQQPDAGTFVKRGSSVQVIFS
                     KGPQRVEVPDLAGKNLPASQAALSGAGLALGQLLGAFSRDAAEGTVLGQDPGPGDAVA
                     PGTPVNMLLVMSVQRERYVMPDLVYRNYDQVLPYFERYGFPLGSVKYERYEGVAAGVI
                     LRQYPLPGHPFTKQDNISLVVATAESPELEPAMITPAEPISR"
     gene            1584..2246
                     /gene="rpe"
                     /locus_tag="VEL74_08485"
     CDS             1584..2246
                     /gene="rpe"
                     /locus_tag="VEL74_08485"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HYG62607.1"
                     /translation="MKLAPSILAADLADLAGAVALCEQGGAEMIHFDVMDGHFVPNLT
                     FGIPVLAALHRRTRLPLDVHLMVEDPDRLLDDYLAAGAARVAVHWEAAPHLDRTLGRI
                     RQKGARAGVAVNPATPVEILVDTLPRLDYVLLMSVNPGFSGQKFLPRALDKARRLAAM
                     IRDAGLSVEIEMDGGIDRGTMRDVVAAGVEVCVVGSGIFAAGDPLATMSELRELARPE
                     RL"
     gene            2243..3058
                     /gene="bamD"
                     /locus_tag="VEL74_08490"
     CDS             2243..3058
                     /gene="bamD"
                     /locus_tag="VEL74_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HYG62608.1"
                     /translation="MKRLFLLLLTLGLTTAIGACRSGGAAEDPILRLSAQESMEIGKD
                     LLAKEKYARARPYLLHAYEVEPNSAMGREALLLSADTFFLEGASQNYVQAEAKYRDFL
                     NRFPTSQQAPYVQFQIANSLAKRMEKPDRDQTTTRKALEAYQELIRIYPTSEYAVQAQ
                     LQIEKVRANLAEHEFVVGRFYMRYGAPGAAIQRFVELLANYPQYGERDKVIYNLGLAY
                     ERSGRSMDAIKTFDRLRAEHPQSSFIAEIPEIRPAAATGGDKDINKEKASEVG"
     gene            3058..>3322
                     /locus_tag="VEL74_08495"
     CDS             3058..>3322
                     /locus_tag="VEL74_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62609.1"
                     /translation="MMRHAGWLLLFCLPALGCAGSALRPDDSTQQEVQELRARLIELQ
                     RKSAMNEVELARLRQQVAEMEGGGGARPASAPATTASRSTSPSA"
BASE COUNT          494 a         1227 c         1117 g          484 t
ORIGIN      
        1 ccgatgtggg cgtgacggtc gcggcgtgag gcgaagggct tcaccgagtc gttgacgtgg
       61 aagcagccga ggacgtccag gcccagccgc tcctccacct cagccaggaa ctcttcacgt
      121 ccggcggcct cgtggatcgg atagccggcg gcgaaagcgt ggcaggtgtc gaggcagacg
      181 ccgacgcggt gcggcgccga gacgagggac cggatgcgct ccaggtgctc caaccggcag
      241 ccgaggcagg tcccctgccc cgccgtgttc tccagcagga cgcgggtggc gatccccggc
      301 atctctgccg gtacagcggc gagcaccctg tccagcgccg cgacgaagct gtcgatcccc
      361 gcctcctccc cctttcccat gtgggccccg gggtggagca ccagcccctg gaccccgaga
      421 cgggcgcagc gcaccagctc gtcggcgagg gcggcctcgg acctttccag gaccagcggg
      481 tcggtcgccg ccaggttgat gagataggag gcgtgggcga ccagcggacc gacggcgctg
      541 gcgccgtggg cggcgcggaa gctctcggcc tcctcgtcgg ccaggtcctt gccgcgccac
      601 tggctggcgt tcttgacgaa gacctgcatc gccgtacact ccagctccgc cgcccgcggg
      661 aaggcgctgc acagcccgcc cgccaccgag acgtgggcgc cgagcggggg gagggccggg
      721 agagtcgggg acgggtcctg ccgtcctggg tgccgtcctg ccatgggccg gcgattgtac
      781 gcccgctttg gtacactgcg ccccatgctc tcgaggctcc tccgcggtct gctctacctg
      841 gtgtacggcc tcgtggtcgt gacgaccttc gccctcgcgg cctatgtctc cttcagcctt
      901 ttcgtgcgca gcggcaccac ccgcgtgccg ccggtgcggg agatggcgcg cgccgaggcc
      961 accgcgctgc tggccgacca ggggctgcgc atccgcgccg ccaaagaggg gcgctatgac
     1021 gacaaggtgc ccgtcggccg ggtggtgcgg cagcagcccg acgcggggac gttcgtcaag
     1081 cgcggcagca gcgtgcaggt catcttctcc aaggggccgc aacgcgtcga ggtgccggac
     1141 ctcgcgggca agaacctgcc ggcgtcgcag gccgccctgt ccggcgccgg cctcgccctg
     1201 ggacagctcc tcggcgcctt ctcgcgcgac gccgcggagg gcaccgtcct cggccaggac
     1261 cccggccccg gcgacgccgt ggcaccgggc acgccggtca acatgctcct cgtcatgtcc
     1321 gtgcagcgcg agcgctacgt catgcccgac ctggtctacc gcaactacga tcaggtgctc
     1381 ccctacttcg agcgctacgg cttcccgctc ggcagcgtca agtacgagcg ctacgagggg
     1441 gtggcggcgg gcgtgatcct gcgccagtac ccgctgccgg gccatccgtt caccaagcag
     1501 gacaacatct ccctggtcgt cgccacggcg gagagccccg agctcgagcc ggcgatgatc
     1561 acccccgcgg agccgatctc ccgatgaagc tcgccccgtc catcctcgcc gccgatctcg
     1621 ccgacctcgc cggggccgtg gccctctgcg agcagggcgg ggccgagatg atccacttcg
     1681 acgtcatgga cgggcacttc gttcccaacc tgaccttcgg catcccggtg ctcgcggcgc
     1741 tccatcgccg cacccggctg ccgctcgacg tccacctcat ggtcgaggac cccgaccggc
     1801 tgctcgacga ctacctggcc gccggcgccg cgcgggtggc ggtgcactgg gaggccgccc
     1861 cccatctcga ccgcaccctc ggccgtatcc ggcagaaggg ggcccgcgcc ggcgtggcgg
     1921 tcaaccccgc gacgccggtg gagatcctgg tggacaccct cccccggctc gactacgtgc
     1981 tcctcatgtc cgtcaacccc gggttttccg gccagaagtt cctcccccgg gctctggaca
     2041 aggcgcgccg gctggccgcg atgatccgcg acgccggcct gtccgtcgag atcgagatgg
     2101 acggcggcat cgatcgcggt accatgaggg atgtggtcgc ggccggagtg gaggtgtgcg
     2161 tcgtgggttc aggaatcttc gcggccggtg atccgttggc gaccatgtcc gagctgcgcg
     2221 agctggctcg gccggagagg ctatgaaacg actctttcta ttgctgctga ccctgggtct
     2281 gaccaccgcc atcggtgcct gccgtagcgg cggcgccgcc gaggacccga tccttcgtct
     2341 ctcggcccag gagtccatgg agatcggcaa ggatctcctg gcgaaggaga agtacgcgcg
     2401 cgcccgcccg tacctcctcc acgcctacga ggtcgagccc aactcggcca tgggacgcga
     2461 ggccctgctg ctgagcgccg acaccttctt cctcgaaggc gcctcgcaga actacgtcca
     2521 ggccgaggcc aagtaccgcg acttcctcaa ccgcttcccc accagccagc aggcgcccta
     2581 cgtccagttc cagatcgcca acagcctcgc caagcggatg gagaagcccg accgggacca
     2641 gaccaccacc cgtaaggccc tggaggccta ccaggagctg atccgcatct atccgaccag
     2701 cgagtacgcc gtccaggccc agctccagat cgagaaggtg cgcgccaacc tcgccgagca
     2761 cgagttcgtg gtcggccgct tctacatgcg ctacggcgcc cccggcgcgg ccatccagcg
     2821 cttcgtggag ctgctggcca actatccgca gtacggcgag cgggacaagg tgatctacaa
     2881 cctcgggctc gcctacgagc gcagcggccg gtccatggac gccatcaaga ccttcgaccg
     2941 gctgcgcgcc gagcacccgc agagttcgtt catcgcggag atccccgaaa tccggcccgc
     3001 cgcggccacg ggcggcgaca aggacatcaa taaggaaaag gcttcggagg taggttgatg
     3061 atgcgacacg ccggatggct cttgctgttc tgcctgccgg ccctgggctg cgccggctcc
     3121 gccctgcgcc cggacgactc gacgcaacag gaggtccagg agctgcgcgc ccggctcatc
     3181 gagctgcagc gcaaatccgc gatgaacgag gtcgagctcg cgcggctgcg tcagcaggtg
     3241 gccgagatgg agggcggcgg cggagcgcgc cccgcctcgg cgcccgccac caccgccagc
     3301 cgcagcacct cgccttccgc cg
//
LOCUS       DASPJI010000320        12461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_419805, whole genome shotgun sequence.
ACCESSION   DASPJI010000320 DASPJI010000000
VERSION     DASPJI010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12461)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12461
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419805"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..2376
                     /locus_tag="VEL74_08500"
     CDS             130..2376
                     /locus_tag="VEL74_08500"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYG62610.1"
                     /translation="MVPPPLGGRAVGSGGGQEGGSLLRRLAAVLALALLATGCNRSGD
                     DTGAVGTFPGSPIIIISIDTLRSDRLPAYGYQGVETPALDALAQDSVLFERAYSHVPL
                     TLPSHASLLTGVLPTRHGVRDNIGYRLDAAKFTPLQEVLRQAGYATGAAVSAYVLRAE
                     TGMDKGFDLYESGIDVKPSEALGRSQRSGGETARRALDWVKTLETQEGKPFFLFLHLY
                     EPHTPYEPPEPFASKYRDRPYDGEIAAVDAVVGGFLDELRQLGTYDDAIIVLTSDHGE
                     GLGEHGEREHGIFLYRESLQVPLLVKLPGARRGGTRVAAPVQLVDVMPTVLSLTGQKA
                     PAREPLDGTSLFEVDKEGAPQRALYAETYYPRLHMGWSELTSVIQDRFHYIHGPDPEL
                     YDMVADTAEAANVLTRERRVYAALRQEAEARERPLQAPAEEDPETAAKLASLGYLGTA
                     SVAEGPLPDPKSRIHTLRDFDVALSHFFRQEYAEAVPAFQRVLAENPRMADGWENLGQ
                     SLQKLGRREEALAAYEKAMEVSGGSGHVALATGSLLLEMGRLGEARAHAELGLPSSPA
                     AARSLLARVALEQGDEATAEREARAALAARGSRIGPLIILAQVLQAQGKLEESLEQTR
                     LAEQELSRMEGDRKFAGLFFVQGDTLARMERFADAERAFQREIADFPGSPGPYSRLAV
                     IYATQGRPAEAVGMLRRMVETNNSPAAYAEAVKTLRILGDPAGASALLRHALTQFPGS
                     PELRALAG"
     gene            2401..4053
                     /locus_tag="VEL74_08505"
     CDS             2401..4053
                     /locus_tag="VEL74_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG62611.1"
                     /translation="MRWTALALVALLSSAPLAGQTAGQTPPSSPAPPAANRAIQEAMQ
                     KVEAGDLAGAIQGLEAAVTPGATPRVTALLGTLYVEAGRAADATRVLKPLADREDAEP
                     AVLYNAGRAALAQGQAAEARRYLERSLAKEPSSPAVRELGLVLSRQGQVVDAYRLLRP
                     WAVRNPRDQEVRLVAATLALRLERPGEAEELAAGFPETDPAIRLLRGEALVQKGDGQG
                     ALAVLAPIREQHPPGMDLEVRRALAEAHLLAGQPAPAVELLAGRAVTPALAAILGRAQ
                     RQAGDLKAAAATLKPLADQLPAGPEGLGDPRPAAAVAREYGLVLRASGEAAAAAAMLE
                     RATRLHPTSVESWQALGEAFAAAGRAGDARAAQAKVRELTAAREAAAKITAERQAAAA
                     REAQTAPGPAPVAGPAVEAQRLLAANKPEEALAAARQGIAAHPDDLELRAAEVRALLL
                     LKRWQDALKAAEAAVKAAPADADAVYLRGAARMALHDRQGAEQDLRRALDLAPRHIAA
                     LNDLGVLLTLQGKKDEAKRMFQRVLEVQPGNPVATASLKQLQ"
     gene            4523..4762
                     /locus_tag="VEL74_08510"
     CDS             4523..4762
                     /locus_tag="VEL74_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006621212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62612.1"
                     /translation="MTSLPQQAIAQIEKLPAADQDAVAARILADLEDEKAWAASFQAT
                     SADQWERLAAMARREIEAGDTVPLEDVFPVKATGE"
     gene            4814..5026
                     /locus_tag="VEL74_08515"
     CDS             4814..5026
                     /locus_tag="VEL74_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009547434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62613.1"
                     /translation="MQEQAAQAYALWVSDPFHPSLQFKRISPSQPIFSVRIGIGYRAL
                     GLRHGEQVDWLWIGSHAEYDALLKRL"
     gene            complement(5154..7442)
                     /locus_tag="VEL74_08520"
     CDS             complement(5154..7442)
                     /locus_tag="VEL74_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG62614.1"
                     /translation="MPKPTGRKKPAARSGTVEPRPSAPAPTRDRGGLPGLAAVFVVAL
                     AARLAALSALGRLVLFRTPQLDSLEYLRWAQSIAAGESRWPLLPTHAPGYPYFLGTLL
                     ALFDGSLTAARFIQALLGAVLCVVVALLGRRLYGPRAGLVAGLLLALYGPLIYVELSL
                     LAEGTFLLCLVLALAALLSPWRSSHPIPAAAVTGLFLGLAALTRATALALLPFFLLLL
                     LLRRGGGSRGPAGVAQATGSVGGGEGGAHTKEPSSAGPAHGWAGAFVMVAVTLLLVIP
                     ATVKVSRVAGSFVPLQAFGGLNLYMGNDPAGTGTPGARLGGDWDHLQAEPLRLGLNAA
                     EQERYFTRKAREVITGDPLGWLAVLGKKALWLIQDDEIRDSHSFHFFAAEAPLLRWLP
                     GWGLLFPLAVCGLWIAARKRSLPPLLAVYLLVMAASCVLIVVASRYRLPLVPVLALFA
                     GLAAVTLWDAARQRRTRDFSLLAGIAVAAWLAGHLWNHEPSRDFTEEWVMSGQSLENE
                     GDLAAAESAYRRALERSPESALAWNGVGKVRVKQGDLAGAEEAFTTATRLDPAYSRSY
                     YNLGIAYRAAGNVERAVAAFRRAVELAPDHVAAMTELGTLLLARGEVMEADTLLRKVV
                     AMDPNNAAANLGLARLEGAAQRPESGVPFAERAVAGAPDDPEAWITLSMLALEAKNAA
                     AAERAVRRLEELMGTDAPGVVLQRATLARLQGRPADADRELRALLQRDPRIEPAARLL
                     LLNAAEQGRRAEAEAFLRELWQ"
     gene            7510..11058
                     /locus_tag="VEL74_08525"
     CDS             7510..11058
                     /locus_tag="VEL74_08525"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYG62615.1"
                     /translation="MIGTRVSNFEILERLGGGGMGVVYKARDLRLDRVVALKFLTARR
                     DGSEGDHQRFLREARAAATLDHPGVCAIHEIGETEDGALFLVMAFCEGESLARVIDSG
                     PLRPAQAAHLAAQIADGLAAAHERGIVHRDIKPANVLVTPGGDAGGHPGGHPGGQIKI
                     VDFGVARLAGVTRITRPGTTVGTAAYMAPEQLRGEEVDARADVWSLGVVLYEMLTGEL
                     PFPGANESEIISGILHAHPRPIGAFRDGVPAGLEHIALRALAKRPADRYARIADMARE
                     LRDFEEAATSVEGTEHTLVALSRSRSGPLPASGLRLGSLERYRVLGPLGGGAMGVVHE
                     AEDTALGRRVALKFLPAELSRDPDSKERFLREARAASALDHPNVCTIHEIGETGDGRL
                     FLAMARYEGETLKRRLERGSVSLEEALDIVRQIAHGLAKAHRQGIVHRDIKPANLMLT
                     GDGVVKILDFGIAKLAGEAGLTRLGTPMGTPAYMSPEQARGDEVGPASDVWSMGAVVY
                     EMLAGRRLFPADNEHAVLYAILHEEPKPLRALRPDVPPELERIVARMLARDPAARYPS
                     AVEAHAEIKALYGPTTSWTVSGVASKEGRWGRWARRLPWMAATLAAGLAAAAYLLPER
                     GRTDAPVQGTFTRLTDQEGSEELPSLSPDGSFFLYTKEEDGNADVYLQRVGGGNPINL
                     TADSPADDTQAVFSPDGQQIAFRSDRDGGGIFLMGATGESARRLTDFGYNPAWSPDGR
                     EILVATEGVSGANVRLSRSEIWRVDAATGARRLLVRGDAVQPSWSPSGRRVAYWGLPA
                     GSGQRVIWTVAADPEKDGAAEPVPVTGDTFLNWNPVWAPDGRHLYYASDRGGSMNLWR
                     VRVDEGSGEVRGAPEPVTTPAPWSGFLSFSRDGKRIVYATRDGRSNLERAPLTDGRAG
                     PLEPVTRGTRTVRSAAVSPDGQWLAFDTSAPQEDLFVVRADGSGQRQLTDDVFKDRSP
                     SWSPRGDRILFYSNRSGRYEAWTIRPDGSGLEQLTRTTGRSLTNPSWSPDGRWLACTL
                     GERGALIDLSKPVAERVPRPLPPVSPAGEVFSPSSWSPDGTALLGYVERPDESPVPGI
                     YLYRLVDGHEGREGRYERLTAGGARAGWLDGGRSVLFSDQGRIFAVDLQSHQVRLVAE
                     PPPHSAFTMASASPDGRALYVVRATDEGDVWRLDLQ"
     gene            11117..11365
                     /locus_tag="VEL74_08530"
     CDS             11117..11365
                     /locus_tag="VEL74_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62616.1"
                     /translation="MAFKIRDLMIDVLPAGDDLPDGRPCHRDSFRPSECHDPSCADCK
                     PHSHCAPPSRLQPEPEGTYAASLPLLREQLRQTLARQI"
     gene            11485..>12461
                     /locus_tag="VEL74_08535"
     CDS             11485..>12461
                     /locus_tag="VEL74_08535"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYG62617.1"
                     /translation="MIGTTVSHYEILERLGSGGMGVVYKARDLTLDRLVALKFLSAHR
                     GGESDRRRFLREARAVSSLEHPNICTVYEVGEAGDGRLFIAMTLCEGEPLKEKLERGP
                     LPPDEAVRLAAGIAAGLAAAHEKGIVHRDIKPGNVMVAPGSPPGSRVKLVDFGIAKLA
                     EETRLTRAGTVVGTAAYIAPEQFRGEAFDHRSDVWALGVLLYEMLTGQLPFDADLQHE
                     LVEAILRREPVPPSILASGVPHELDRVVLRALAKRPADRYPDMKALREDLERLLASPE
                     ELGKTVVAIPSSRSWPITPSPADPSGTGSRRGSPSLSSQTGPRYEILEPV"
BASE COUNT         1774 a         4657 c         4498 g         1532 t
ORIGIN      
        1 cgacatccgc aaggcgcaga acctgctcca gggggcagga tgaggccctc accccctggc
       61 cccctctccc gcctccccaa cccccttccc gggagagggg gaagaggaaa ctctagtcag
      121 cagggggcgg tggtgcctcc ccccctggga gggcgggcgg tagggagcgg gggaggtcag
      181 gaggggggtt ctctcctgcg gcgcctcgcc gccgtcctcg ccctcgccct cctggccacg
      241 ggctgcaacc gctccggaga cgataccggt gccgtgggga ccttccccgg ctcccccatc
      301 atcatcatct ccatcgacac gctacgctcc gaccgcctgc cggcctacgg ctaccagggg
      361 gtggagacgc cggccctcga cgccctggcg caggattccg tcctcttcga gcgcgcctac
      421 agccacgtgc cgctgacgct gccgtcgcac gcctcgctcc tcaccggcgt gctccccacc
      481 cgccacggcg tgcgcgacaa catcggctac cggctggacg ccgcgaagtt cacccccctc
      541 caggaggtgc tccgccaggc gggctatgcc accggcgcgg cggtgtcggc ctacgtgctg
      601 cgcgccgaga ccggcatgga caaggggttc gacctctacg agtcggggat cgacgtcaag
      661 ccctcggagg cgctcggccg cagccagcgg agcggcggcg agaccgcgcg gcgggcgctc
      721 gactgggtga agaccctgga gacgcaggag gggaagccgt tcttcctctt cctccacctc
      781 tacgagccgc acacccccta cgagccgccg gagccgttcg cctcgaagta ccgggaccgc
      841 ccctatgacg gcgagatcgc cgccgtcgac gcggtggtcg gcggcttcct cgacgagctg
      901 cggcagctcg ggacctacga cgacgccatc atcgtcctca cctcggacca cggcgagggg
      961 ctgggggagc acggcgagcg cgagcacggc atcttcctct accgcgagtc gctccaggtg
     1021 ccgctgctgg tcaagctccc cggcgcccgg cgcggtggca cgcgggtcgc ggcgccggtc
     1081 cagctcgtgg acgtcatgcc cacggtcctg tccctcaccg gccagaaggc gccggccagg
     1141 gagccgcttg acggcacctc cctcttcgag gtggacaagg agggggcgcc ccagcgggcg
     1201 ctctacgcgg agacctacta tccgcgcctg cacatgggct ggagcgagct gacctcggtg
     1261 atccaggacc gcttccacta catccacggg ccggaccccg agctgtacga catggtggcc
     1321 gacaccgccg aggcggccaa cgtcctcacc cgggagcgcc gggtctacgc ggccctgcgc
     1381 caggaggccg aggcgcggga gcgcccgctc caggcgccgg ccgaggagga cccggagacg
     1441 gcggccaagc tcgcctccct cggctacctg ggcacggcgt ccgtggccga ggggccgctc
     1501 cccgatccca agagccgcat ccacacgctg cgcgacttcg acgtcgccct gagccacttc
     1561 ttccggcagg agtacgccga ggcggtgccg gccttccagc gggtgctggc ggagaacccg
     1621 cggatggcgg acggctggga gaacctgggg cagtcgctcc agaagctcgg ccgcagggag
     1681 gaggcgctcg ccgcctacga gaaggcgatg gaggtctccg gcggctccgg gcacgtcgcc
     1741 ctggccaccg gctcgctgct gctggagatg ggcaggctcg gcgaggcccg ggcccacgcc
     1801 gagctggggc tgcccagcag cccggcggcg gcgcgcagcc tgctcgcccg cgtcgccctc
     1861 gagcaggggg acgaggccac ggcggagagg gaggcgcggg cggcgctggc ggcgcggggc
     1921 tcgcgcatcg ggccgctgat catcctcgct caggtgctgc aggcgcaggg gaagctcgag
     1981 gagtcgctgg agcagacgcg cctcgccgag caggagctct cccgcatgga gggggaccgc
     2041 aagttcgccg gcctcttctt cgtccagggg gacacgctgg cgcgcatgga gcgcttcgcc
     2101 gacgccgagc gggccttcca gcgcgagatc gccgacttcc ccggcagccc cggaccctac
     2161 tcgcgcctgg cggtgatcta tgcgacccag gggcggccgg cggaagcggt cggcatgctc
     2221 cgccgcatgg tggagaccaa caactccccc gccgcctacg ccgaggcggt caagacgctc
     2281 cgcatcctcg gcgacccggc cggcgcctcc gccctcctgc gccacgccct cacccagttc
     2341 cccggcagcc cggagctgcg cgcgctcgcc ggatagacgg ccggatagga gacctgactc
     2401 atgagatgga ccgccctcgc tctcgtagcc ctcctctcct ccgctcccct cgccggccag
     2461 acggctggac agacgccccc ctcctcgccg gcgccgccgg ccgccaaccg cgccatccag
     2521 gaggcgatgc agaaggtgga ggccggcgac ctggccggcg ccatccaggg gctggaagcc
     2581 gccgtgactc ccggcgcgac cccgagggtg acggcgctcc tcggcacgct ctacgtcgag
     2641 gccggccgtg ccgcggacgc cacgcgggtg ctgaagccgc tggcggaccg cgaggacgcc
     2701 gagcccgccg tgctctacaa cgccggccgc gccgccctcg cccaggggca ggccgccgag
     2761 gcgcggcgct acctggagcg ctcgctcgcc aaagagccgt cctcgccggc ggtccgcgag
     2821 ctggggctcg tgctgtcgcg ccagggccag gtggtggacg cctaccggct gctccgtccg
     2881 tgggccgtgc gcaatccgcg ggaccaggag gtcaggctcg tcgccgccac cctggcgctg
     2941 cgcctggagc ggccgggcga ggccgaggag ctggccgccg gcttcccgga gaccgacccc
     3001 gccatccgcc tgctccgtgg cgaggcgctg gtccagaagg gggacggcca gggggccctg
     3061 gcggtgctgg cgccgattcg cgagcagcac ccgccgggca tggacctgga ggtccgccgc
     3121 gccctggcgg aggcgcatct cctcgccggc cagcccgccc cggcggtcga gctgctcgcc
     3181 ggccgcgccg tcacgccggc cctcgccgcg atcctcggcc gggcccagcg ccaggcgggg
     3241 gacctgaaag cggcggcggc caccctgaag ccgctggccg accagctccc cgccggaccc
     3301 gaggggctgg gagatccgcg cccggccgcc gcggtcgccc gggagtacgg cctggtgctc
     3361 cgcgcctcgg gcgaggccgc cgccgcggcc gccatgctgg agagggcgac gcggctccat
     3421 ccgacgagcg tcgaatcgtg gcaggcgctg ggcgaagcct tcgccgccgc cggccgggcc
     3481 ggcgacgccc gcgccgccca ggcgaaggtc cgggagctga ccgccgcccg cgaggcggcg
     3541 gcgaagatca ccgccgagag gcaggccgcg gcggcccggg aggcccagac agcgccgggg
     3601 ccggcgccgg tcgccggccc cgccgtcgag gcccagcgtc tgctcgccgc gaacaagccc
     3661 gaggaggcgc tggccgccgc ccgccagggg atcgcggccc atcccgacga cctggagctg
     3721 cgcgccgccg aggtgcgcgc gctcctcctc ctcaagcgct ggcaggacgc cctgaaggcc
     3781 gccgaggcgg cggtcaaggc cgcgcccgcc gacgccgacg ccgtctatct gcgcggcgcc
     3841 gccaggatgg ccctccacga ccgccagggc gccgagcagg acctgcgccg cgccctcgat
     3901 ctcgctcccc ggcacatcgc cgcgctgaac gacctcggcg tgctcctcac cctccagggg
     3961 aagaaagacg aggcgaagcg gatgttccag cgggtgctgg aggtccagcc cggcaatccg
     4021 gtggcgacgg cgagcctgaa gcagctccag taggcctcac cccccggcct ccgctatctc
     4081 gtcccggcca tctggatccc atcccttcga gctggacgcg atccgaggcg ctcgggaagc
     4141 ttcccgagcg ctccggatgc cgtccaggcg gctcggatgc agtccgaagc aggcggaagc
     4201 ttcccggatc gtccggacac cgtccgaacg gcctggacgc gatccgcaac ggcgggaagc
     4261 ttcccggagc gtccggaggc cgtcccggcg gtctggacgc cgtccgagac gttcaggaag
     4321 cctcttcaag cgtccgggcg gggtcgacgg cgccgtcagc tcggcggcgg gaccgtagtc
     4381 cggaacccaa tgattccgcg ggtcctcctt cactttctgg atctcgtcgg gagtgggagt
     4441 gaagaggccc gggcgcagtg cagtgccctg tacaatgagg gagcgccagg agccttgcgc
     4501 tgccaggaaa ggaaaagacg agatgacgag cctcccgcaa caggctatcg cccagatcga
     4561 gaagctgccc gcggcagacc aggatgccgt cgctgcccgt atccttgcgg atctggagga
     4621 tgaaaaagcc tgggcggcga gcttccaagc cacctccgcg gatcagtggg agcgtttggc
     4681 ggcgatggcc cgacgggaga tcgaagcagg cgataccgtg ccgctggagg atgtgttccc
     4741 ggtcaaggcg accggcgagt gaagtagcgt ctccaaggag ttcagaaaga ggctcgaccg
     4801 gctccctgcc accattcaag agcaggcagc gcaggcgtac gcgctctggg tctcggatcc
     4861 ctttcacccg agcttgcaat tcaagcggat cagcccatca cagccgatct tctcggtccg
     4921 catcggcatc ggctatcggg ccctcggcct gcggcacgga gagcaggtgg actggctctg
     4981 gatcggctcc cacgcggaat acgacgccct cctgaagcgg ctctgaggat cggcaacccc
     5041 agccagaggc cgctcgctcc acctcagcca gtgctctctc ccacagctac aggagccgct
     5101 cgccttcaga cagaagcctc tgggagcggg agagggggcc ggggggtgag ggcctactgc
     5161 cacagctccc gcaggaacgc ctccgcctcc gcccgccgcc cctgctcggc ggcgttgagg
     5221 aggagcagcc gggcggcagg ctcgatgcgg gggtcgcgct ggagcagcgc ccgcagctcg
     5281 cggtcggcgt cggcggggcg gccctggaga cgggcgaggg tggcgcgctg gaggacgacc
     5341 cccggcgcgt ccgtccccat cagctcctcc aggcgccgga cggcccgctc cgccgccgcg
     5401 gcgttcttcg cttccagggc cagcatggac agcgtgatcc aggcctccgg atcgtccggc
     5461 gctcccgcca ccgccctctc ggcgaagggg actccactct ccggccgctg cgccgcccct
     5521 tcgagacggg cgaggcccag attggcggcg gcgttgttcg ggtccatcgc caccacctta
     5581 cgcagcagcg tgtcggcctc catcacctcc ccgcgggcga gcagcagcgt ccccagctcg
     5641 gtcatcgcgg cgacgtggtc gggcgccagc tccacggcgc gccggaacgc ggcgacggcc
     5701 ctctccacgt tcccggcggc gcggtaggcg atcccgaggt tgtagtagct gcgggagtag
     5761 gcggggtcga gccgggtggc ggtggtaaaa gcctcctcgg cgccggcgag gtccccctgc
     5821 ttgacgcgga ccttgccgac gccgttccag gccagggcgg actcggggct ccgttcgagg
     5881 gcgcggcgat aggcggactc ggcggcggcc agatccccct cgttctccag cgactggccg
     5941 ctcatcaccc actcctcggt gaagtcgcgg ctcggctcgt ggttccagag gtgcccggcg
     6001 agccaggcgg cgacggcgat cccggcgaga agggagaaat cgcgggtgcg ccgctggcgg
     6061 gcggcgtccc agagcgtcac ggcggcaagg ccggcgaaga gggcgagaac cggcacgagg
     6121 gggagacgat agcgggaggc caccacgatc agcacacagg aggcggccat cacgaggagg
     6181 tagacggcga gcagcggggg aagagagcgc ttccgggcgg cgatccagag gccgcagacc
     6241 gccaggggga agaggaggcc ccagcccggg agccagcgga ggaggggcgc ctcggcggcg
     6301 aagaagtgga agctgtggct gtcgcggatc tcgtcgtcct ggatcagcca gagggccttc
     6361 ttgcccagga cggcgagcca gcccagggga tcgccggtga tgacctcgcg ggctttgcgg
     6421 gtgaagtagc gctcctgctc ggcggcgttg aggccgaggc gcaggggctc ggcctggagg
     6481 tggtcccagt cgccgccgag gcgggcgccg ggggtgccgg tgccggcagg gtcgttcccc
     6541 atgtagaggt tgagacctcc gaatgcctgg agggggacga agctgcccgc cacgcgggag
     6601 accttgacgg tggccgggat gaccagaagg agggtcaccg cgaccatgac gaaggccccc
     6661 gcccagccgt gggccggacc ggcgctgctc ggctccttcg tgtgcgctcc cccctctccc
     6721 ccacccacgc tgccagtcgc ctgcgccacg ccggccggcc cacggctccc cccaccccgg
     6781 cgcaggagga ggagaaggag gaaaaagggg aggagcgcca gggcggtggc tcgcgtcagg
     6841 gctgcgagac ccaggaagag gccggtgacg gcggctgccg gaatgggatg ggaagaacgc
     6901 cagggggaga ggagcgccgc cagcgccaga accaggcaga gcaggaaggt cccctcggcc
     6961 aggagggaga gctcgacgta gatcaggggg ccgtagagcg cgaggagcag accggcgacg
     7021 aggccggcgc gtgggccgta gaggcggcgc cccaggaggg ccacgacgac gcagaggacg
     7081 gcaccgagga gggcctggat gaaccgtgcg gcggtgaggg agccatcgaa gagggccagg
     7141 agggtgccga ggaagtaggg gtagcccggc gcgtgggtcg gcagcagcgg ccaacgggac
     7201 tcgccggcgg cgatgctctg cgcccagcgc aggtactcca gggagtcgag ctgcggggtc
     7261 cggaagagga cgagccgccc gagggcggag agggcggcca gccgcgccgc caaagccact
     7321 acgaagacgg cggcgaggcc gggaaggccg ccgcggtcac gcgtgggagc gggcgcagac
     7381 ggccgcggct cgacagtgcc ggagcgggcg gcgggcttct tgcggccagt cggtttcggc
     7441 acggtctggt agactacacc ggattccgag cagccaccct tccggagtcg ttccttccga
     7501 gccagtcgtg tgatcggcac ccgcgtctcc aacttcgaga tcctcgagcg tcttggcggc
     7561 ggaggtatgg gcgtggtcta caaggcccgg gacctgcgcc tggaccgcgt cgtcgccctc
     7621 aagttcctga ccgcccggcg cgacggcagc gagggggacc accaacgctt cctgcgcgag
     7681 gcgcgcgccg ccgccaccct cgaccacccg ggggtctgcg ccatccacga gatcggcgag
     7741 accgaggacg gcgccctgtt cctggtcatg gccttctgcg agggggagag cctggcccgc
     7801 gtcatcgact ccggccccct ccgcccggcc caggcggccc acctcgcggc ccagatcgcc
     7861 gacggcctcg ccgccgccca cgagcgcggg atcgtccacc gcgacatcaa gccggccaac
     7921 gtcctggtca ccccgggcgg cgatgccgga ggacatccgg gaggacatcc gggcggtcag
     7981 atcaagatcg tcgacttcgg cgtcgcccgg ctcgccggcg tcacccgcat cacccgcccc
     8041 ggcaccaccg tcggcaccgc cgcctacatg gcccccgagc agctccgcgg cgaggaggtg
     8101 gacgcccgcg ccgacgtctg gtccctcggc gtcgtcctct acgagatgct caccggcgag
     8161 ctgcccttcc ccggcgccaa cgagtccgag atcatcagcg gcatcctcca cgcccacccg
     8221 cggcccatcg gcgccttccg cgacggggtg cccgccggcc tcgagcacat cgccctgcgc
     8281 gccctggcca agcggcccgc cgaccgctac gcgcggatcg ccgacatggc gcgcgagctg
     8341 cgggacttcg aggaggccgc cacctcggtg gaggggaccg agcacaccct cgtggccctc
     8401 tccaggagcc gctccggccc cctccctgcc tccgggctcc ggctcggctc gctggagcgc
     8461 taccgggtcc tcggcccgct cggcggcggc gccatggggg tcgtccacga ggccgaggac
     8521 acggcgctcg ggcggcgggt ggcgctcaag ttcctgccgg cggagctgag ccgcgacccc
     8581 gactccaagg agcgcttcct gcgcgaggcg cgggcggcgt cggccctcga ccaccccaac
     8641 gtctgcacca tccacgagat cggcgagacc ggcgacggcc ggctcttcct cgccatggcc
     8701 cgctacgagg gggagaccct caagcggcgg ctggagcgcg gctcggtctc cctggaggag
     8761 gcgctggaca tcgtccgcca gatcgcccac gggttggcca aggcccaccg ccaggggatc
     8821 gtccaccgcg acatcaagcc ggccaacctg atgctcaccg gcgacggcgt ggtcaagatc
     8881 ctcgacttcg gcatcgccaa gctcgccggc gaggccggcc tcacccgcct cggcaccccc
     8941 atgggcacgc cggcctacat gtcgcccgag caggcgcggg gcgacgaggt ggggccggcg
     9001 agcgacgtct ggtcgatggg cgccgtggtc tacgagatgc tcgccggccg ccgcctcttc
     9061 cccgccgaca acgagcacgc ggtcctctac gccatcctcc acgaggagcc gaagccgttg
     9121 cgcgccctgc ggccggacgt gccgcccgag ctggagcgca tcgtcgcccg catgctcgcc
     9181 cgcgacccgg cggcacgcta cccctcggcg gtggaggccc acgccgagat caaggcgctc
     9241 tacggcccca ctacgagctg gaccgtctcc ggcgtcgcct cgaaggaagg gcggtggggg
     9301 cggtgggcgc gccggctccc ctggatggcg gcgaccctgg ccgcggggct ggcggccgcc
     9361 gcctacctcc tgccggagag agggcggacg gacgccccgg tccagggcac cttcacccgc
     9421 ctcaccgacc aggaggggag cgaggagctc cccagcctgt cgccggacgg cagcttcttc
     9481 ctctatacca aggaggagga cggcaacgcc gacgtctacc tgcagcgggt gggcggcggc
     9541 aacccgatca acctcaccgc cgactcgccg gcggacgaca cccaggccgt cttctccccc
     9601 gacggccagc agatcgcctt ccggtccgac cgcgacggcg gcggcatctt tctcatgggc
     9661 gccaccggcg aatcggcgcg ccggctgacc gacttcggct acaacccggc ctggtccccc
     9721 gacggccggg agatcctggt ggcgaccgaa ggggtcagtg gcgccaacgt ccggctgtcc
     9781 aggagcgaga tctggcgcgt cgacgccgcc accggcgccc gccggctcct ggtgcggggg
     9841 gacgccgtgc agccgagctg gtcccccagc ggccggcgcg tcgcctactg ggggctgccc
     9901 gccggcagcg gccagcgggt gatctggacc gtggcggccg acccggagaa ggatggggcc
     9961 gccgagccgg tgccggtcac cggcgacacc ttcctcaact ggaacccggt ctgggcgccg
    10021 gacggccgcc acctctacta cgccagcgac cgcggcggca gcatgaacct ctggcgggtg
    10081 cgcgtggacg agggctccgg cgaggtgcgg ggcgcgcccg agccggtgac cacgccggcc
    10141 ccctggagcg gcttcctcag cttctcgcgc gacggcaagc ggatcgtcta tgccacccgc
    10201 gacggccggt cgaacctgga gcgcgcgccg ctgacggacg gccgggccgg ccccctggag
    10261 ccggtgacgc gcggcacccg caccgtccgc tcggccgccg tctcccccga cgggcagtgg
    10321 ctcgccttcg acacctcggc gccccaggag gatctgttcg tcgtgcgcgc cgacggcagc
    10381 ggccagcgcc agctcaccga cgacgtgttc aaggaccgct ctccgagctg gtcgccgcgc
    10441 ggcgaccgca tcctcttcta ctcgaaccgc agcggccggt acgaggcctg gacgatccgc
    10501 ccggatggca gcggcctgga gcagctcacc cgcaccacgg gccggtcgct gaccaatccg
    10561 tcgtggtccc cggacggccg ctggctcgcc tgcactctgg gggagcgcgg ggcgctgatc
    10621 gacctctcga agccggtcgc cgaacgggtg ccccgccccc tgccgccggt cagcccggca
    10681 ggggaggtct tctcgcccag ctcctggtcg ccggacggca ccgccctcct cggttatgtc
    10741 gagcggccgg acgaaagccc cgtgccgggc atctatctct accggctcgt tgatggccac
    10801 gagggccgcg agggccgcta cgagcggctc accgccggcg gcgcccgcgc gggctggctg
    10861 gacggcggcc gcagcgtcct gttcagcgac cagggccgga tcttcgccgt cgacctccag
    10921 agccaccagg tccgcctcgt ggcggagccg cccccccact ccgccttcac catggcgagc
    10981 gcgagcccgg acggccgcgc cctctacgtg gtgcgcgcca ccgacgaagg ggacgtctgg
    11041 cgcctcgatc tccagtgagg cttccagtga cggcgggtcc ggccgcgggt atactggccg
    11101 ccacgggagg gataccatgg ctttcaagat ccgagacctg atgatcgacg tgctccccgc
    11161 gggtgacgat ctgcccgacg ggcggccctg ccaccgggac tcgttccggc cgtccgagtg
    11221 ccacgatccg agctgtgccg actgcaagcc gcacagccat tgcgcgccgc cctcgaggct
    11281 ccagccggaa ccggaaggga cgtacgccgc gagcctccct ctgctccggg agcagctccg
    11341 ccagaccctg gcccggcaga tctagctccc gcggcccccg cggaggcgtc ccgcgacggc
    11401 tctggtagac tttctatcca gagatcggaa gacccctacg tccaccgtcc cgtcccctcg
    11461 gggcctgcga ggaagcctcc gcggttgatc ggcaccaccg tctcccacta cgagatcctg
    11521 gagcgcctgg gcagcggcgg gatgggcgtg gtctacaagg cccgcgacct gaccctcgac
    11581 cggctggtgg cgctcaagtt cctctcggcc caccgcggcg gcgagtccga ccgccgccgc
    11641 ttcctgcgcg aggcgcgggc ggtgtcctcc ctggagcacc ccaacatctg caccgtctac
    11701 gaggtgggcg aggccggaga cggccggctg ttcatcgcca tgacgctgtg cgagggggag
    11761 ccgctcaagg agaagctgga gcgcggcccc ctccccccgg acgaagcggt gcgcctcgcc
    11821 gccggcatcg ccgcgggcct cgccgccgcc cacgagaagg ggatcgtcca ccgcgacatc
    11881 aagccgggca acgtcatggt ggcacccggc agcccccccg gcagtagggt gaagctggtg
    11941 gacttcggca tcgccaagct cgccgaggag acccggctca cccgcgccgg cacggtggtg
    12001 ggcaccgcgg cctatatcgc ccccgagcag ttccggggcg aggccttcga ccaccggtcg
    12061 gacgtctggg ccctgggcgt gctcctctat gagatgctca ccggccagct ccccttcgac
    12121 gccgacctcc agcacgagct ggtggaggcg atcctccgcc gcgagcccgt gccgccgtcg
    12181 atcctggcct ccggcgtgcc gcacgagctg gaccgcgtcg tgctgcgcgc cctggccaag
    12241 cggccggcgg accgctatcc cgacatgaag gcgctgcgcg aggacctgga gcggctgctg
    12301 gcgagcccgg aggagctggg gaagacggtg gtggcgatcc cctcgtcccg ctcgtggccg
    12361 atcaccccgt ctccggcgga cccctccggc accggcagcc ggcgcggctc cccgagcctg
    12421 tcgtcgcaga cgggaccgcg ctacgagatc ctggagccgg t
//
LOCUS       DASPJI010000321         5520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_78566, whole genome shotgun sequence.
ACCESSION   DASPJI010000321 DASPJI010000000
VERSION     DASPJI010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78566"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1529
                     /gene="gltX"
                     /locus_tag="VEL74_08540"
     CDS             66..1529
                     /gene="gltX"
                     /locus_tag="VEL74_08540"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HYG62618.1"
                     /translation="MSTDNDGPVRVRFAPSPTGFLHVGGVRTAIYNDLLRLRRGGAFV
                     LRIEDTDRERSDEAMTRQIQDALSWTGVTWDEGPFLQSERLPRHRERGEELLAAGKAY
                     RCFCTTEILEAQRAEAQKQGAAFRYPRTCLGLSQQEVEERLAAGTPFVVRFRMPDEPI
                     RFTDLVRGDMEFPPEALDDFILLRSDGTPTYHMSVVVDDIDMDITHVIRGEDHLSNTP
                     KHVPLFQALGGRVPTFGHLPLILGTDKKRLSKRTGATSVEEFRAEGVFPQALYNYMAL
                     LGWSPGDDREIMTRQEMAATFSTDRLKASAAVFDREKLLWMNGQYMSSLPVDQILPYF
                     EPFLEEVGLTGADRQRLLQALDLHRSRARALKELAPMMLPYFRDELLYDREACAKFLK
                     DPDLPVHLAALRDRYAALEPFEKGGLENELRVLAEDRGIKAGVLIHPTRMALTAAATG
                     PSLFDLVEVMGRDATVRHLAHFVAYLRKGGEEVIHQD"
     gene            1599..1672
                     /locus_tag="VEL74_08545"
     tRNA            1599..1672
                     /locus_tag="VEL74_08545"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1631..1633,aa:Gln,seq:ctg)
     gene            complement(1730..1924)
                     /locus_tag="VEL74_08550"
     CDS             complement(1730..1924)
                     /locus_tag="VEL74_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62619.1"
                     /translation="MRPTLLPIVQGVLAAHPAETERYRAGQTGVLGFFVAQVMKQAGQ
                     EGGKPNPKLVNAILTGELGA"
     gene            complement(1934..3730)
                     /locus_tag="VEL74_08555"
                     /pseudo
     CDS             complement(1934..3730)
                     /locus_tag="VEL74_08555"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
     gene            complement(3820..4050)
                     /locus_tag="VEL74_08560"
     CDS             complement(3820..4050)
                     /locus_tag="VEL74_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62620.1"
                     /translation="MAWITATTVDKKNVNLQSQQIAAILDRVSGSPAKGSEVILASGK
                     TLEVSESPAELLRAIEQERHPENPPVPPDEAW"
     gene            4127..5425
                     /locus_tag="VEL74_08565"
     CDS             4127..5425
                     /locus_tag="VEL74_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62621.1"
                     /translation="MVAETGSAALPAPEWSPATRILFRFAFVYFLLYYLPTPFSLIPF
                     VGVLFAPIESFWSMLVPWVGSHFFAVEITDLMPNGSGDRTYGYVRVFCGLVLSLAATL
                     AWTLLDRKRPSYPRLREWLWIFLRFSLGMALIGYGAYKVIKSQFPDPSLDRLLQPFGN
                     ASPMGLLWTFMGASDAYTIFGGACEMLGGLLLTFRRTTLLGALVSASVMLNVVMLNFS
                     YDVPVKIYSTHLFLTAVVLALPDLRRMADLFLFNRRVEPVAERPLFERRWLDRSALGL
                     RTAFVVLVTATSLYQSHQTRTMYGDDAPKSPLYGVWTVERIETDGQIRPALITDASRW
                     RRVIFEVPGRMSIQSMDDSRARYSLDPKAGANVLAMSKRDDPKWTSKISYRETSPGVM
                     MLQGTFDGQKIRAVLRKTEADDFLLVNRGFHWINEAPFNR"
BASE COUNT          796 a         1931 c         1840 g          953 t
ORIGIN      
        1 ggttcgaatc cctccctctc cgccatcctc tttccgcttc ctcggcctcc tcggagaccc
       61 gcaccatgag cacggacaac gacggcccgg tccgggtgcg tttcgcgccc tcccctaccg
      121 gtttcctcca tgtcggcggc gtccgcaccg ccatctacaa cgatctgctg cggctgcggc
      181 gcggcggcgc cttcgtcctc cgcatcgagg acacggaccg ggagcgctcg gacgaggcga
      241 tgacccgcca gatccaggac gccctctcct ggaccggcgt cacctgggac gaggggccgt
      301 tcctgcagag cgagcgcctc ccccgccacc gggagcgcgg cgaggagctg ctggcggccg
      361 gcaaggccta ccgttgcttc tgcaccaccg agatcctgga ggcccagcgc gccgaggccc
      421 agaagcaggg cgccgcgttc cgctatcccc gcacctgcct gggtctcagc cagcaggagg
      481 tcgaggagcg gctcgccgcc ggcaccccgt tcgtggtccg cttccgcatg cccgacgagc
      541 cgatccgctt caccgacctg gtgcggggcg acatggagtt cccgccggag gcgctggacg
      601 acttcatcct cctgcgctcc gacggcacgc cgacctacca catgtcggtg gtagtggacg
      661 acatcgatat ggacatcacc catgtcatcc ggggcgagga ccacctctcg aacaccccca
      721 agcacgtgcc gctgttccag gcgctgggcg gccgggtccc caccttcggc cacctgccgc
      781 tgatcctcgg gaccgacaag aagcgcctgt cgaagcgcac cggggcgacg tcggtcgagg
      841 agttccgggc cgagggggtc ttcccccagg cgctctacaa ctacatggcg ctcctcggct
      901 ggagcccggg ggacgaccgg gagatcatga cccggcagga gatggcggcg accttctcca
      961 ccgaccggct caaggcgtcg gcggcggtgt tcgaccgcga gaagctcctc tggatgaacg
     1021 gccagtacat gtcgtccctg ccggtggacc agatcctccc ctacttcgag ccgttcctcg
     1081 aggaggtggg gctgaccggc gcggaccgcc agcggctgct ccaggcgctg gacctccacc
     1141 gcagccgcgc ccgcgccctc aaggagctgg cgcccatgat gctcccctac ttccgggacg
     1201 agctcctcta cgaccgggag gcctgcgcca agttcctcaa ggacccggac ctgccggtgc
     1261 acctggcggc gctgcgcgac cgctacgccg ccctggagcc gttcgagaag ggtggcctcg
     1321 agaacgagct gcgggtgctg gccgaggatc gggggatcaa ggccggggtg ctcatccacc
     1381 ccacccgcat ggcgctgacc gccgcggcga ccggtccctc cctcttcgat ctggtggagg
     1441 tcatgggccg cgacgccacg gtccgccacc tggcccactt cgtcgcctac ctccggaaag
     1501 ggggcgagga ggtcattcac caggattgag gcaggatcga gagccgaacg ggattgacgg
     1561 agccgttccg tttgtttatc ctctcgggcc tgtcctgctg ggaggtcgtt caacggtagg
     1621 acatgcggct ctgacccgca gaatcggggt tcgaatccct gcctcccagc caattcgctg
     1681 atcccagtcc gaggccgccg tcaggcggcc tcggtgttta tggcctcggc taggcgccca
     1741 gctccccggt caggatcgcg ttgaccagct tggggttggg cttgccgccc tcctgccccg
     1801 cctgcttcat cacctgggcg acgaagaagc cgaggacccc cgtctggccg gcgcggtagc
     1861 gctcggtctc ggcgggatgc gccgcgagca ctccctggac gatgggcagg agggtggggc
     1921 gcatccgctc cagcagctcc agctcctcgg gcgagagcgg cgtgggggcc ttcggctcct
     1981 gcgccttggg cgccttcggc tcggccggct tctgcggagc gggtgccgcg gcgggcgcct
     2041 gcttcttggc ctcctgcgcc caggagtccc ggagctgcac gatccggttg aagacgaggc
     2101 gatcagggcg cgtatcgacg ggatcgcgga agaagtagcc ctgccgctcg aactgcagcc
     2161 gctctcccat ctccggatcc gccgccgccg gctccaggcg gctgccggtc agcgtctcca
     2221 gggcgttcgg gttcaggtac tcgtcgaagc tcccctcctc gaggtccggc cgctcgaccc
     2281 ggaacaggcg gtcgtagagc cgtacctcca ccggcaccga gtgctcggcc gagacccagt
     2341 gcagggtgcc gggcaccttg cggccgtctg gcggcgagcc gccacgggtc gccgggtcat
     2401 aggtgcagcg cacctcgacc acctcgccgg tcgccgggtc cttgaccacg ctctcgcagc
     2461 ggacgaagta ggcgtagcgc agccgtacct ccttgcccgg ggagaggcga tggaacccct
     2521 tcggcggcac ctccgcgaag tcgtcccgct cgatgtagag gacgcgcgag aagggcacct
     2581 tgcggctccc ctccttgggc acgtcatgcg ggtagagcgg cgcgtccagc tcctccacct
     2641 ggccctcggg gtagttctcg atcaccagct tcagcggccg cagcacgcac agcacccggg
     2701 ggacctcggg gttgagggcc tcgcgctggc agtgctcgag cagcgtcacg tccaccgtgc
     2761 tgttcgcctt ggcgacgccg acccgctcgc agaggtcgcg gatcgcctcg ggggtgaagc
     2821 cgcggcgccg catgccggcc agggtgggca tgcgcggatc gtcccagccc gtcaccagcc
     2881 tgcgctgcac cagctcgagg agcttgcgct tgctcatgat cgtgtaggtg aggttcaggc
     2941 gggcgaactc gatctgcttc ggcgggtccg ggatctccag cgtctccatc agccagtcgt
     3001 agatgtcccg gttgttctcg aactccaggg tgcagaacga gtgggtgatc ccctcgatgg
     3061 cgtccgacag cgggtgggcg aagtcgtaca tcgggtagat ggaccaggcg tcgccccggt
     3121 ggtagtgggt ggcgcgcttg atgcggtaga acagggggtc gcgcatcttc atgttgggcg
     3181 acgccatgtc gatcttggcg cgcagcacgt gggcgccgtc cgggaactcg ccatcccgca
     3241 tccgccggaa caggtcgagg ttctcctcga ccgagcggtc acggtacggg ctgttccggc
     3301 ccgcctcggt gacggtgccg cggtactcgc ggatctcgtc ctcgttcagg ctgtcgacgt
     3361 aggccttgcc ccggcggatc agctcgacgg cgaagtcatg gagccgctcg aagtagtcgg
     3421 aggtgtggaa gaagttctct ccccagtcga agcccagcca gcacacgtac tcctggatcg
     3481 cgtcgacgta ctcctgggac tcggtctcgg gattggtgtc gtccagacgg aggttgcacc
     3541 ggccgccgtt ctcctgggcg atgccgaagt tgaggcagat cgacttggcg tggccgatat
     3601 gcagatagcc gttcggctcc gggggaaaac gggtgaccac ccgcccgccg tgcttgccgg
     3661 cggcgcgatc gcggttgacc aggtcgcgaa tgaagtcgcg ccctggagta taggagacag
     3721 gttcggacat accagtgatc ctcggaggaa taggaggccc ccggtccgaa acggaccgtg
     3781 gagccgcccc catcctataa gagaagagac ggaggccgcc taccaggctt cgtccggggg
     3841 caccggcgga ttttccgggt gccgctcctg ctcgatggcg cgcaggagct cggccgggct
     3901 ctcgctgacc tcgagggtct tccccgacgc gagaatgacc tcgctcccct tggcgggaga
     3961 gcccgacacc cggtccagga tggcggcgat ctgctgggat tgcaggttga cgttcttctt
     4021 gtcgacggtc gtcgcggtga tccaggccat gctccggagg atactacgtc ccgccagggc
     4081 ctcggccatg ctagccttga tcaggagatc tcaggaggtg cattgcatgg ttgccgagac
     4141 cggttccgcg gcgctccccg cccccgagtg gagccccgcg acgcggatcc tgttccgctt
     4201 cgcgttcgtc tacttccttc tgtactacct gccgaccccc ttctccctga tcccgttcgt
     4261 gggtgtgctg ttcgcgccga tcgagagctt ctggtccatg ctcgtgccgt gggtgggcag
     4321 ccatttcttc gcggtcgaga tcaccgatct gatgccgaac ggcagcggcg accggaccta
     4381 tggctacgtg cgggtgttct gcggcctggt cctgagcctg gcggcgaccc tggcctggac
     4441 cctgctcgac cgcaagcggc cgagctatcc gcggctccgc gagtggctgt ggatcttcct
     4501 ccgcttctcc cttgggatgg cgctcatcgg gtacggcgcc tataaagtga tcaagtcgca
     4561 gttccccgac ccgagcctcg accgcctgct gcagccgttc ggcaacgcct cgccgatggg
     4621 cctcctgtgg acgttcatgg gcgcctccga cgcctacacc atcttcggcg gcgcctgcga
     4681 gatgctcggc gggctgctgc tcaccttccg gcgcaccacg ctgctgggag ccctggtctc
     4741 cgccagcgtc atgctcaacg tcgtcatgct caacttcagc tacgacgtgc cggtgaagat
     4801 ctattcgacc cacctgttcc tgaccgccgt cgtcctcgcc ctgcccgacc tgcggcggat
     4861 ggcggacctg ttcctgttca accggcgggt ggagccggtg gcggagcgcc cgctcttcga
     4921 aaggcggtgg ctcgaccgga gcgccctggg gctccgcacc gccttcgtcg tcctcgtcac
     4981 cgcgacgtcg ctgtaccagt cccaccagac ccggacgatg tacggcgacg acgcgcccaa
     5041 gtcgccgctc tacggcgtct ggaccgtgga gcggatcgag accgacggcc agatccgtcc
     5101 ggcgctgatc accgacgcga gccgctggcg gcgggtgatc ttcgaggtcc ccggcaggat
     5161 gtccatccag tccatggacg acagccgcgc gcgctacagc ctcgatccga aagccggcgc
     5221 caacgtgctc gccatgtcga agcgcgatga tccgaaatgg acctccaaga tttcgtaccg
     5281 cgagacctct cccggagtga tgatgctcca gggaacgttc gacggccaga agatccgcgc
     5341 cgtgctccgc aagacggagg cggacgattt cctgctcgtc aaccgcggct tccactggat
     5401 caacgaggcg ccgttcaacc ggtgacgcgc cgccagcccg gcctgatgga gctcggcggc
     5461 gcgccacgcg tcggtcgcgc cggcgcctac ttcttcttgg tgtccttctt ggtggcggcg
//
LOCUS       DASPJI010000322         4720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_826059, whole genome shotgun sequence.
ACCESSION   DASPJI010000322 DASPJI010000000
VERSION     DASPJI010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826059"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4720
                     /locus_tag="VEL74_08570"
     CDS             <1..>4720
                     /locus_tag="VEL74_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62622.1"
                     /translation="IPALLAVLEAGAAYVPLEIGHPPARLQWIAGALDISCVLTESAQ
                     RGSLPELPHVICLDRVELERERPVAPPRRGAPDDLAYIIFTSGSTGTPKGVMVRHRPV
                     VNLFRWAYRAFDFSPADRVLFVTSLAFDLSVFDVFGLLGAGGSVRIATEEEIRDPRLL
                     VRALAEEPITFWDSAPAALEQTVPFLAALEPEARPSLRLVFLSGDWIPVTLPDRVRER
                     FPEARVISLGGATEATVWSNVFPIGTVDPVWKSIPYGRPIDNARYHVLDARLAPCPMG
                     VPGDLYIGGDCLADGYAREPELTAGKFLPDPWSATRGGRLYRTGDRARYRPDGNLEFL
                     GRLDHQVKIRGFRIELGEIEAALATLPGVREAVVVVREDAPGDRRLVAYVTGDALVDD
                     LRPWLRERLPESMLPSAFVSLAALPLTPNGKVDRKALPAPDRQRAEEAYVPPHTPVEE
                     IVAGIWAEVLGLERVGAADHFFELGGHSLLATQVVSRLRRAFGVELPLRDLFEAPTLD
                     DLAARIEVAARAGSRPLAPPLVPLAPAVREGALPPSFAQQRLWFIDQLAPGSPLYNIP
                     VALRVEGPLDARVLALCLEEIVRRHEVLRTVFAAPDGSPVQVIRPPEPFELPVVDLAA
                     LPCPGRETAALIAEEALRPFDLARGPLSRGLLLRLADGDHVIALTLHHIAGDAWSMGI
                     LVREVTALYPAFTQGRPSPLPDLPVQYADFAVWQSSWLRGDLLEHEISFWRRQLAGLP
                     PLLELPTDRPRPAAQSLRGATRPVRLPSGLARRLEALTRRAGGTLFMTLLAGFQALLA
                     RYSGQQDLAVGSPVAGRNRVEVERLIGFFVNTLVLRGDLSGGPSFGELLGRARETVLA
                     AHAHQDLPFERLVQELAPERSLAHTPLFQAMLVLQSAPVEAPAIPGLRLRLAGVAAAT
                     AKFDLLLTLEERDGGLAGTVEHATELFDGATIDRLIGHFERLLEGAAARPELPVAELP
                     ILSGVERGQILTEWNDTLSEPALRGCAHELFAAWARRAPEAVAAVYGAEALTYAELRS
                     RAGHLARHLRRLGVGPDVLVGLMAERSLDMAVGVLGILEAGGAYVPLDPGYPEQRLAW
                     MLADTRSPVLLTQRHLRHRLPAGDSAIVLLDGGGWSEAPGGAVPAAAGPSEESLAYVI
                     FTSGSTGRPKGVALPHGALRNLIDSHLAGLVGGARTLQFASLSFDVSFLEMFACWASG
                     GTLVVVPEELRRDLPALTGLLAAAEVEKAIFPGAVLQQLAEIYAGRDALPPLREIIAT
                     GERLQTNQAMAALFRRLPGCAFHNHYGPSETHVATAFTLSADPAEWAVYPSIGRPIRN
                     AATYVLEPGRIPAPAGVPGELFIGGVGGACLARGYLRRPDLTAERFVPDPFGGEPGAR
                     LYRTGDKVRLLAGGDLEFLGRFDDQVKIRGFRIEPGEIEQALVALPGVREAVVVARED
                     ALGQRRLVAYVVGDAAPETLRQSLRERLPDYMVPAAVVKLDELPLSPNRKVDRKTLPS
                     PEWQGAGESYRAPRTPVEEVLAGIWTELLGPERVGLDDHFFDLGGHSLLATQVASRLR
                     STFAVEMPLRELFEAPRLADL"
BASE COUNT          537 a         1756 c         1703 g          724 t
ORIGIN      
        1 gatcccggcc cttctcgccg tgctcgaggc gggcgccgcc tacgtgccgc tggagatcgg
       61 ccacccgccg gcccgcttgc agtggatcgc cggcgcgctg gacatctcct gcgtgctcac
      121 cgagtcggcg cagcggggct ccctgccgga gctgccgcac gtgatctgtc tggatcgcgt
      181 ggagctggag agggagcggc cggtggcgcc gccgcgccga ggggctccgg acgacctcgc
      241 ctacatcatc ttcacctcgg gctccacggg cacgcccaag ggggtgatgg tccgccaccg
      301 cccggtggtc aacctgttcc gctgggccta ccgcgccttc gatttctccc cggcggaccg
      361 ggtgctcttc gtcacctccc tggccttcga cctgtcggtc ttcgacgtct tcggcctgct
      421 gggggccggc ggctcggtcc gtatcgccac cgaggaggag atccgcgatc cccgtctcct
      481 ggtgcgcgcc ctggccgagg agcccatcac cttctgggac tcggcgcccg ccgccctgga
      541 gcagacggtg ccgttcctgg cagcgctgga gccggaggcg cgcccgtccc tccggttggt
      601 cttcctgagc ggcgactgga tcccggtgac cctgccggac cgcgtccgcg agcgcttccc
      661 cgaagcccgg gtgatctccc tgggcggcgc caccgaggcc accgtctggt ccaacgtctt
      721 ccccatcggg acggtcgatc cggtctggaa gagcatcccg tacggccggc ccatcgacaa
      781 cgcgcgctat cacgtgctcg acgcgcggct cgccccctgc ccgatgggtg tgccgggaga
      841 cctctatatc ggcggggact gcctggccga cggctatgcc cgggagccgg agctgaccgc
      901 cggcaagttc ctacccgacc cctggagcgc cactcgcggc ggccggctct accgcacggg
      961 cgaccgcgcc cgctaccgtc cggacggcaa tctggagttc ctggggcggc tggaccacca
     1021 ggtcaagatc cgcggcttcc gtatcgagct gggggagatc gaggcggctc tcgccaccct
     1081 tcccggcgtg cgcgaggcgg tggtggtggt gcgggaggac gcccccggcg accgccggct
     1141 ggtcgcctac gtgaccggcg atgccctggt ggacgatctc cgcccctggc tgcgggagcg
     1201 gctgccggag tccatgttgc cctcggcctt cgtctccctg gcggcgttgc ccctgacccc
     1261 gaacggcaag gtggaccgca aggcgctgcc ggcgccggac cggcagcgcg ccgaagaggc
     1321 ctacgtgccg ccgcacacgc cggtcgagga gatcgtggcc gggatctggg cggaggtgct
     1381 cggtctcgag cgagtggggg cggccgacca cttcttcgag ctgggcggcc actcgctcct
     1441 ggcgacccag gtggtgtcgc ggctgcggag ggccttcggt gtcgagctgc cgctgcgcga
     1501 tctgttcgag gcgcccaccc tggacgacct cgccgcccgc attgaggtag cggcgcgggc
     1561 cggctcgcgc cctctcgctc cacccctggt ccccctcgcc ccggcagtgc gggagggagc
     1621 gttgcccccg tccttcgccc agcagcgcct ctggttcatc gatcagctcg cgccgggaag
     1681 cccgctctac aacatccctg tggcgctacg cgtcgaaggc ccgctggacg cccgcgtgct
     1741 ggccctctgc cttgaggaga tcgtgcgccg gcacgaggtc ctgcgcacgg tcttcgcggc
     1801 gccggacggc tcacccgtgc aggtgatccg tccgccggag cccttcgagc tgccggtggt
     1861 ggacctcgcg gctctcccgt gccccggccg cgagaccgcc gcgctgatcg ccgaggaggc
     1921 cctccgcccg ttcgacctcg cccggggacc gctgtcccgc ggcctgctgc tgcggctggc
     1981 ggatggcgac catgtcatcg cgttgaccct gcaccacatc gctggtgacg cgtggtccat
     2041 gggcattctg gtccgcgagg tcacggctct ctatccggcg ttcacccagg gcagaccctc
     2101 ccccttgccg gacctcccgg tgcagtacgc ggactttgcc gtctggcaga gctcctggct
     2161 gcgcggtgac ctcctggagc acgagatctc cttctggcgc cgccagctcg cgggcctgcc
     2221 gcctctcctg gagctcccca ccgaccggcc gcggcccgcg gcgcagagcc tccgcggcgc
     2281 gacgcgtccg gtgcggctgc cgtccgggct cgcccggcgg ctcgaggcgc tcacccggcg
     2341 cgcggggggg acgctcttca tgacgctcct cgccggcttc caggccctgc tggcgcgcta
     2401 cagcggccag caggacctgg cggtgggctc gccggtcgcc ggacgcaacc gggtggaggt
     2461 cgaaaggctg atcggcttct tcgtcaacac cctggtgctg cggggggacc tgtcgggggg
     2521 cccctccttc ggcgagctgc tcggtcgcgc ccgcgagacc gtgctcgccg cccacgcgca
     2581 ccaggacctg ccgttcgagc ggctcgtcca ggagctcgcg ccggagagga gcctcgccca
     2641 cacccctctg tttcaggcga tgctcgtcct gcagagcgcg ccggtggaag cgccggcgat
     2701 cccgggcctg cgcctgcggc ttgcgggagt ggccgcggcc acggcgaagt tcgatctcct
     2761 gctcaccctc gaagagcgcg acggcgggct ggccggcacg gtggagcacg ccaccgagct
     2821 gttcgacggt gcgaccatcg accgcctcat cggccacttc gaacggctgc tcgaaggcgc
     2881 cgcggcgagg ccggagctgc cggtggcgga gctgccgatc ctcagcggcg tcgagcgcgg
     2941 gcagatcctg accgagtgga acgacaccct ctcggagccg gctctccgcg gctgcgcgca
     3001 cgagctgttc gcggcgtggg cgcggcgggc tccggaggcc gtggccgcgg tctacggggc
     3061 ggaggcgctg acctatgccg agctccgctc ccgcgccggg catctggccc ggcacctgcg
     3121 ccgcctcgga gtcgggccgg acgtcctggt cgggctcatg gccgagcgct ccctggacat
     3181 ggccgtcggt gtgctgggca tcctcgaggc gggcggcgcg tatgtgccgc tcgatcccgg
     3241 ataccccgag cagcgcctgg cctggatgct cgcggacacc cgctctcccg tcctgctgac
     3301 acagcggcac ctgcggcatc ggctgccggc cggagactcc gcaatcgtcc tgctcgacgg
     3361 cggcgggtgg agcgaagctc ccggcggcgc ggtgccggcc gcggcggggc cttccgagga
     3421 gagcctggcg tatgtgatct tcacctcggg ctccaccggc cggcccaagg gggtggccct
     3481 cccgcatgga gcgctgcgca acctcatcga ctcgcacctg gcgggcttgg tgggcggagc
     3541 gcggacgctc cagttcgcct cgctgagctt cgacgtcagc ttcctcgaga tgttcgcctg
     3601 ctgggcctcc ggcggcaccc tggtggtcgt gccggaagag ttgcgccgcg acctgcccgc
     3661 cctgaccggg ctgctggcgg cggcggaggt cgagaaggcc atcttccccg gggcggtcct
     3721 gcagcagctc gcggagatct atgccggccg ggacgcgctg ccgccgctcc gcgagatcat
     3781 cgcgacgggc gagcggttgc agacgaacca ggcgatggcg gcgctgttcc gccggctccc
     3841 gggctgcgcc ttccacaacc actacggccc gtccgaaacc cacgtcgcca ccgccttcac
     3901 cttgagcgcc gacccggcgg agtgggcggt ctacccctcc atcggccggc cgatccggaa
     3961 tgccgccacc tacgttctgg agccgggccg gattccggct cccgcgggcg tcccggggga
     4021 gctctttatc ggcggcgttg gcggcgcctg tctggcccgc ggctacctga ggcggcccga
     4081 cctcacggcc gagaggttcg tgcccgatcc gttcggcggc gagccggggg cccggctgta
     4141 ccggacgggg gacaaggtgc ggctcctcgc cggtggagac ctggagttcc tcgggcgctt
     4201 cgacgaccag gtcaagatcc gcggcttccg catcgagccg ggagagatcg agcaggcgct
     4261 cgtcgccctc cccggtgtcc gtgaggcggt ggtggtggcg cgggaggacg cgctcggcca
     4321 gcggcggctg gtggcctacg tggtcggcga cgccgcgccg gagacgctgc gccagtcgct
     4381 acgcgagcgg ctgccggact acatggtgcc ggcggccgtc gtgaagctgg acgagctccc
     4441 cctcagcccg aaccgcaagg tcgaccgcaa gaccctgccc tctcccgagt ggcagggtgc
     4501 cggcgagagc taccgggcgc cgcggacgcc ggtggaggag gtcctcgcgg gcatctggac
     4561 cgagctcctc ggccccgaac gggtcgggct ggacgaccac ttcttcgacc tgggcggcca
     4621 ctcgctgctg gcgacccagg tggcctcgcg cctgcgtagc accttcgcgg tggagatgcc
     4681 gctgcgcgag ctgttcgagg cgccgcggct ggcggacctc
//
LOCUS       DASPJI010000323         7840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_591019, whole genome shotgun sequence.
ACCESSION   DASPJI010000323 DASPJI010000000
VERSION     DASPJI010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7840)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591019"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..419
                     /locus_tag="VEL74_08575"
     CDS             81..419
                     /locus_tag="VEL74_08575"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HYG62623.1"
                     /translation="MNTMQNINTTQPVARKLIRPRGEKLKAERVQEKLRSMPGWQLAR
                     EGKALSRVREFPDHGVATAFASYAGAFTARMKQPADVTLSGSRVLVILHARSRAGLSN
                     AVLEFAKSLG"
     gene            476..883
                     /locus_tag="VEL74_08580"
     CDS             476..883
                     /locus_tag="VEL74_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62624.1"
                     /translation="MPRVLTRQGDVRKELEDILAPLEANAADLPQLEVHRLAIVDVLQ
                     DIRSFTADQDIFRANKQQTSKRLRAAIDKGRKLLTVARVVLKQHYGSDNEKLLEFGVQ
                     PFRGRSRAQTPAPETPEDKPQDRLKAKPSEPES"
     gene            1071..1475
                     /locus_tag="VEL74_08585"
     CDS             1071..1475
                     /locus_tag="VEL74_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62625.1"
                     /translation="MSRAELPDEIRRFILTSVPSVPYLEAILLLRIDPDVAWDVRRVA
                     ARLYVAERQAAEVLTALAAAGIAQGEDHGDTALFRYAPATDELRERLDALAQAYSANL
                     VGVTDLIHSRLDKRAQQFADAFRVRKDSGRED"
     gene            1477..1737
                     /locus_tag="VEL74_08590"
     CDS             1477..1737
                     /locus_tag="VEL74_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HYG62626.1"
                     /translation="MAPLIYSLCALTSLACCVLLWRGWRAGGNRLLFWSALCFAGLAV
                     NNLLLVIDKVVLPTQVDLQIWRLASAFLSVSLLLFGLVWEEE"
     gene            1740..1997
                     /locus_tag="VEL74_08595"
     CDS             1740..1997
                     /locus_tag="VEL74_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HYG62627.1"
                     /translation="MQQMLMGGIAMGWALAGLFFFRFWRQSRDRFFLFFAASFWIESI
                     NRVALGMRESASETNPMFYGIRLVSYGLILLAIWQKNRPRS"
     gene            2123..2197
                     /locus_tag="VEL74_08600"
     tRNA            2123..2197
                     /locus_tag="VEL74_08600"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2156..2158,aa:Glu,seq:ttc)
     gene            2271..4691
                     /locus_tag="VEL74_08605"
     CDS             2271..4691
                     /locus_tag="VEL74_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG62628.1"
                     /translation="MSAIVRDLRHGLRTLRKSPGFAAVTILTLGLGIGATTAIFSVVD
                     SVLLRPLPFQEPERLVMVWNRFAALDLEEVPFSEPEFLQYREQAKSFEGMAAYRTPSM
                     TIRGEQGPERVTGAMTTASFFDVLGVRPLHGRGFRPQEDQPNAPFVAVLSHAAWERLY
                     GRDPALVGRTIYVDGRPTEVVGIMPPGFDFPADTEIWLPVPFLATNPLQARFNQTFAR
                     LQPGVSLESARAELTAIAARVQQALPDEYLPTSGWGTTAVPLQEQIVGDMRERLLVLL
                     AAVGLLLLLACANVANLLLTRAQARGREAALRTALGASRGRLVGQFLTESALLALLGG
                     LLGLLLAQAGLTAVQALDPGNIPRLDEVRLDGRVLAFALGVSLLTGLLLGTLPALQSR
                     LDLSEIFKQGGRTLAGSGSRMARRGLVVAEIAIGLVLVVGAMLLGKSLFALQEVDPGF
                     DPKNVLTLRVSLPPQQYPDPPRLVQFYDRLLERLRSMPGVEAAGAVSHLPLSGIDFSA
                     SFTVETESEPTGEMPPEGEFRAVTMDYFKAMGIPLRQGRSFQITDQPDTTLVAVVDEA
                     LVRRYWPDRNPLGQRLKFGPPEIQTTPWMTVVGVVASVKNQALDGDSRAQIYYPVTQA
                     PPSPSMFVAVRGERDPEALAPMVRQAIREIDPEVPVYEVASMEERVSRSLSRPRFSVI
                     LTGTFAGFALLLAALGIYGVIAYSVTERTQEIGVRMALGAERRQVLGLVIRQGMLLAA
                     LGLVVGALLAMGLTRWLSSQLFGISAADPAVFAGVAMLLALVALLATWLPARRATRVD
                     PVVALRYE"
     gene            complement(4702..>7840)
                     /locus_tag="VEL74_08610"
     CDS             complement(4702..>7840)
                     /locus_tag="VEL74_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioester reductase domain-containing protein"
                     /protein_id="HYG62629.1"
                     /translation="RRAGVSSFGMGGTNVHCILEEAPDPGPPAPARPWQLLLFSARTE
                     TALETATDRLADHLETQPEQSLADIAFTLQAGRRAFQHRRALVCRTREEAVAGLAGRD
                     PRRLLSAVQAPGERPLVWMLSGLGEHYPRMGEGLYREEPTFRDALDRCAELLRPLLGQ
                     DLRELLYPGGVRAEDEGGSGGGMDLRRLLGRASDPAGSAGHLRETRIAQPAVFAVGYA
                     LSRLLLEWGLSPRALVGYSLGEYTAACLAGVFSLEDAVRVVAERARLIQELPAGAMLA
                     IPFSEEQTLPLLGTGLSLAAVNGPAYSIVAGPVEEVSALEGRLQGMGLTSRRLETTHA
                     FHSRMMEPVASRLAALLAGIALRPPEIPLISNVTGTWMTAAEATDPGYWTRHLCQAVR
                     FGAGLEELWREPGRLLLEVGPGQALTTLATQHPASAAAAEAGAPVIRTMRHPHERRPD
                     SSALLEAVGRLWLSGVPVDWQGFHAHESRRRVELPTYPFERQTFYIAARTAPWTGAPA
                     AGGPPPRSEPEAAAALAAMPAAGLNLHPRPALRHAFVEPRTGTEQEIAAIWQRILGIR
                     DIGAHDSFFELGGNSLVVPQLLIAVRERFGVDLPVAALFDVPTVAELAGAVETVQREG
                     ADALAVDRKPVDLRAEVVLTSEISGAGLPMADWQRPRGLVLTGATGFLGAYLLRDLLE
                     LTEARVFCPARAASDGEAEQRIRRNLESRGLWRDEYTARIVPLAADLGLPQWGLAAAD
                     FDRLAAETDAIYHAGAWVNFTYPYQVLKPINVDGTVEALRLASRQRLKAVHFISSVAA
                     ISQRVFAGGGAALEDAELEHTDGLFGGYGETKWVGEQIVRLAAARGIPVTVHRPGVVS
                     GDTRTGSGNTRDMIWNMMKGSIQLGLAPGTDFPIDVTPVDFVSRAIVHLSLRRESLSH
                     AFHFPNPSPMRWTEALHILQRYGYEIEAVPYARWLAKVAEVVPADPGHALFPFLPILV
                     PADLMGQPERILEALPAATDATPGPLSEPRYDMANTRAGLEGSGIACSPVDENLLRTY
                     LDAFVGMGWLPAPSR"
BASE COUNT         1214 a         2721 c         2682 g         1223 t
ORIGIN      
        1 atctggtggt gctggcggcc cttcataacc gatcaacttt ccgtggccac gaaggcccgg
       61 aattctaaag gagaagtgtc atgaacacga tgcagaacat caacaccacg cagccggtcg
      121 ccaggaagct gatccgcccc cggggcgaga agctgaaggc ggagcgggtc caggagaagc
      181 tgcgctcgat gccgggctgg cagctcgccc gggagggcaa ggcgctgagc cgggtccgcg
      241 agttcccgga tcacggtgtg gccacggcct tcgccagcta cgcgggagcc ttcaccgccc
      301 gcatgaagca gccggccgac gtcaccctct cgggttcgcg ggtgctggtg atcctccacg
      361 cgaggagccg cgccgggctg tccaacgccg tgctcgaatt cgccaagtct ctgggttgac
      421 ccgggatttc cacggaacca cgaacgaaac acgactcaac gaaaaggaga aaaccatgcc
      481 cagagtactc acgcgtcagg gagacgtccg caaggagttg gaggacatcc tggcacccct
      541 ggaagccaac gcggcagatc tgcctcagct cgaggttcac cgcctcgcca tcgtggacgt
      601 gttgcaggac atccgctcct tcacggccga tcaggacatc tttcgcgcca acaagcagca
      661 gacgtcgaag cggctgaggg ccgccatcga caagggccgg aagctgctga cggtggctcg
      721 ggtcgtcctc aagcagcact acgggtcgga caacgagaag ctgctggaat tcggggtgca
      781 gccgttccgc ggccgttccc gcgctcagac gccggccccg gagactccgg aggacaagcc
      841 gcaggacagg ctcaaggcca agccctccga gcccgaatcc tgacgtgccg ttgatccact
      901 gaaccaccgg ggcgtgcacc gcacgccccg gtacctgctc cttcagtcgt ttaggacaag
      961 ctcgctcttt gacaattgaa ggacacctcc ggagcgcgga gccctcagcg gacgggggcg
     1021 ccgcgctctt tggatgtgct gctcgggctg gcaagtataa gatctcctgg atgagtcgcg
     1081 ccgagctccc ggatgagatc cgacggttca tcctgaccag cgtgccctcg gttccctacc
     1141 tcgaagccat cctgcttctg aggatcgatc ccgatgtggc ctgggacgtg cggcgagtgg
     1201 ccgcgcgcct ctacgtggcc gagaggcagg ccgccgaggt gctcaccgcg ctagccgcgg
     1261 ccggcatcgc gcaaggcgag gaccatggag acaccgccct gttccgctat gcgcccgcca
     1321 cggacgagct gcgcgagcgt ctcgacgctc tggcgcaggc gtactcggcc aacctggtcg
     1381 gggtgaccga cctgatccat tcgcggctcg acaaacgggc acagcagttc gccgatgcct
     1441 tccgcgtgcg caaggacagc ggaagagagg actgatatgg cacctttgat ctactccctc
     1501 tgcgccctga ccagcctcgc ctgctgcgtg ctgctgtggc ggggctggcg cgcggggggc
     1561 aaccgtctgc tgttctggag cgcgctgtgc tttgccggac tggcggtaaa caacctgctg
     1621 ctggtgatag acaaggtggt actgccgact caggtggacc tgcagatctg gcggctggct
     1681 tcagcttttc tgtcggtgtc gctcttgctg ttcggcctgg tctgggagga agagtagcca
     1741 tgcagcagat gctgatgggc ggcatcgcca tgggctgggc gctcgcgggc ctgttcttct
     1801 tccgcttctg gcgccaatcg cgtgaccggt tcttcctctt cttcgctgcc tccttctgga
     1861 tcgagtcgat caatcgcgtg gcactgggaa tgagggagag cgccagcgag accaatccga
     1921 tgttctacgg catccggctg gtctcctatg gcctgatcct gctggcgatc tggcagaaga
     1981 accggccgcg ctcctgaagt gctataccta ccgacaggat ccctgggtgc atacatctgc
     2041 gggtccgatg agcctatctg tgctaaccta ctgcgcggtt tggcgggcgg ctctttcctc
     2101 gggagctatc gcggtccgtc gtgtccccat cgtctagtgg ttaggactcc gccctttcaa
     2161 ggcggcaaca cgggttcgaa tcccgttggg gataccagct cacatccagc ccccttcgcc
     2221 ggtgatccgg gcgggcgaaa tcaccacgaa agcggtctag aatcggcctc atgagcgcga
     2281 tcgtgcgaga cctccggcac ggattgcgaa cgcttcggaa gagcccgggg ttcgccgccg
     2341 tcaccatcct cactctcggc ctcggcatcg gggccaccac ggccatattc agcgtcgtcg
     2401 acagcgtcct gctgcgcccc ctgcccttcc aggagccgga gcggctcgtc atggtgtgga
     2461 accgattcgc ggccctggac ctcgaggagg tgcccttctc cgagcccgag ttcctccagt
     2521 accgggagca ggccaagagc ttcgagggta tggccgccta ccgcacgccc agcatgacga
     2581 tccggggcga gcaggggccc gaacgggtga ccggcgccat gaccaccgcc tccttcttcg
     2641 acgtcctcgg cgtccggccc ctgcacgggc ggggcttccg tccacaggaa gaccagccga
     2701 acgccccgtt cgtcgcggtg ctcagccacg ccgcctggga acgcctctac ggcagggatc
     2761 cggcactggt gggcaggacg atctacgtcg acggcaggcc caccgaggtc gtcggcatca
     2821 tgccgccagg cttcgacttc ccggccgaca cggagatctg gctgccggtg ccgttcctcg
     2881 caacgaaccc gctgcaggcc cgcttcaacc agacctttgc ccggctccag ccgggtgtct
     2941 ccctggagag tgcccgagcc gagttgacag ccatcgccgc ccgcgtgcaa caggccctcc
     3001 ccgacgagta cctgccgacc agcggctggg gcaccaccgc cgtgccgctg caggagcaga
     3061 tcgtcggcga catgcgcgag cgcctgctgg tgctcctcgc cgcggtcggt ctcctgctgc
     3121 tccttgcctg cgccaatgtc gcgaacctgt tgctcacccg cgctcaggcc cgaggacggg
     3181 aggctgcgct acgcaccgcc ctgggcgcca gccgcggccg gctggtgggg cagttcctca
     3241 ccgaaagcgc tctcctcgcc ctgctcggcg gtctgctggg cctcctgctg gctcaggcag
     3301 gcctcaccgc cgttcaggcg ctcgatcccg gcaatatccc acggctggac gaggtgaggc
     3361 tcgatggccg cgtcctcgcc ttcgccctcg gcgtctcgct gctgaccggg ctgctcctgg
     3421 ggacgctccc cgccctgcag tcgcgcctgg acctctccga gatcttcaag caggggggaa
     3481 ggaccctcgc cggctccggc agccggatgg cgcgccgcgg cctggtcgtg gccgagatcg
     3541 ccatcggact ggtgctggtg gtcggcgcca tgctcctggg caagagcctg ttcgccctcc
     3601 aggaggtgga ccccggtttc gatccgaaaa acgtccttac cctgcgggtg agcctgcctc
     3661 cccagcagta cccggatccg ccccgcctcg tccagttcta cgatcgactt ctggaacgtc
     3721 tgcggtcgat gcccggtgtc gaagccgccg gtgcggtgtc tcatctgccc ctttccggta
     3781 tcgacttctc ggcctccttc accgtcgaga ccgaatccga gcccaccggc gagatgccgc
     3841 ccgaagggga attccgggcg gtcacgatgg actatttcaa ggccatgggg atcccgctac
     3901 ggcagggtcg atccttccag atcaccgacc agccggacac gaccctcgtc gccgtggtcg
     3961 acgaggcgct cgtccgcagg tactggcccg accgcaaccc gctgggccag cggctcaagt
     4021 tcggcccccc cgagatccag acgacaccct ggatgacggt ggtcggcgtg gtagcgagcg
     4081 tcaagaacca ggcgctggac ggcgactccc gagcccagat ctactacccg gtcacccagg
     4141 cgcctcccag tcccagcatg ttcgtggctg tgcgcggtga gcgtgacccg gaggccctcg
     4201 cgccgatggt ccggcaggcg atccgcgaga tcgatccaga ggtgccggtc tacgaggtgg
     4261 cgagcatgga ggagagagtc tccaggtcgc tatcgcgccc ccgcttctcc gtcattctca
     4321 ccgggacctt cgccggtttt gccctgctcc tggctgccct cgggatctac ggcgtcatcg
     4381 cgtactcggt gaccgaacgg acacaggaga tcggtgtccg catggccctg ggggccgagc
     4441 gcaggcaggt gctgggcctg gtgatccgcc agggcatgct gctggcggcg ttggggctcg
     4501 tggtaggcgc gctcctcgcg atgggactga cacgctggct gtcgagccag ctcttcggta
     4561 tcagcgcggc ggacccggcg gttttcgcgg gggtcgccat gctcctcgcc ctggtggcgc
     4621 tcctggccac ctggctcccg gcccgccgtg ccacccgggt cgacccggtg gtggcgctac
     4681 ggtacgagta gagcctctcc gtcagcggga gggggccggc agccagccca tgcccacgaa
     4741 ggcatccagg taggtgcgga gcaggttctc gtccaccggc gagcaggcga tgccgctccc
     4801 ctccagcccc gcccgtgtgt tggccatgtc gtagcgcggc tcgctcaggg ggccgggtgt
     4861 cgcatccgtg gcagccggca gggcctccag gatccgctcg ggctgcccca tgagatccgc
     4921 cggcacgagg atcgggagga aggggaagag ggcatgcccc gggtctgccg gcaccacctc
     4981 ggcgaccttg gccagccagc gcgcataggg aaccgcctcg atctcgtacc cataacgctg
     5041 caggatgtgg agtgcctcgg tccagcgcat gggcgaaggg ttggggaagt ggaaagcgtg
     5101 gctcagcgac tcccgccgca gcgacaggtg cacgatggcc cggctgacga agtccaccgg
     5161 cgtgacgtcg atggggaaat cggtccccgg cgccaggccg agctggatgg agcccttcat
     5221 catgttccag atcatgtccc tggtattgcc cgagcccgtc cgggtatcac cggagaccac
     5281 gccggggcgg tggacggtga ccgggatccc ccgcgccgcc gccaggcgga cgatctgctc
     5341 ccccacccac ttggtctcgc catagccgcc gaagaggccg tcggtgtgct ccagctccgc
     5401 gtcttcgagg gcggcgccgc cgccggcgaa gacccgttga gagatggccg ccaccgagga
     5461 gatgaagtga accgccttga gccgctgccg gctggcgagg cgcagggcct cgaccgtgcc
     5521 gtcgacgttg atcggcttga gcacctggta ggggtaggtg aaattgaccc aggcacccgc
     5581 gtgataaatc gcgtcggtct cggcggccag acggtcgaag tccgcggcgg cgaggcccca
     5641 ttgcggcagc cccaggtcgg ccgccagggg cacgatccgc gccgtgtatt cgtctcgcca
     5701 caggccgcgg ctctcgaggt tgcgccggat gcgctgctcc gcctccccat cgctggcggc
     5761 gcgggccgga cagaagacgc gggcttccgt cagctccagc aggtcacgca gcaggtaggc
     5821 gccgagaaag ccagtggcgc cggtgaggac gaggccgcgc ggccgctgcc aatccgccat
     5881 gggcaggccg gcgcccgaga tctcggacgt cagcactact tcggcgcgga ggtcgacggg
     5941 cttgcgatcc accgccagag cgtcggcgcc ctcgcgctgc acggtctcca cggcaccggc
     6001 gagctcggcc acggtgggga cgtcgaacag ggcagccacc ggcaggtcga ccccgaagcg
     6061 ctcgcgtacg gcgatgagga gctgcgggac caccagggag ttgccgccca actcgaagaa
     6121 gctgtcatga gcgccgatgt ctcggatacc caggatgcgc tgccagatcg cggcgatctc
     6181 ctgctcggtg ccggtccgcg gctccacgaa ggcgtggcga agggcggggc gcggatgcag
     6241 gttgagcccg gccgccggca tcgccgccag cgccgcggcc gcctccggct cggagcgggg
     6301 tggcggcccg ccggcggccg gtgccccggt ccacggtgcc gtcctcgccg cgatgtagaa
     6361 ggtctggcgc tcgaaaggat aggtggggag ctcgacacgc cggcggctct cgtgagcgtg
     6421 gaagccctgc cagtccaccg gcaccccaga gagccagagg cgccctaccg cctccagcag
     6481 cgccgacgag tccggccgtc tttcatgagg gtggcgcatg gtgcggatca ccggcgcgcc
     6541 ggcctcagcc gccgcggcac tcgccgggtg ctgcgtcgcc agggtggtga gggcctggcc
     6601 cggcccgacc tccaggagga ggcggcccgg ctcgcgccac agttcctcga gccctgctcc
     6661 gaagcgcacc gcctggcaca gatggcgcgt ccaatacccc gggtcggtcg cttccgccgc
     6721 cgtcatccag gtgccggtga cgttggagat cagcgggatc tccggcggcc ggagagcgat
     6781 gccggcgagg agcgcggcga ggcgggaggc caccggttcc atcattcgag agtggaaggc
     6841 gtgggtggtc tccaggcgcc ggctggtgag ccccattccc tgcaagcgcc cctcgagcgc
     6901 tgatacctcc tccaccggac cggcgacgat gctgtacgcc gggccattga ccgccgccag
     6961 cgacagaccg gtgcccagga ggggaagggt ctgttcctcg gaaaacggga tcgccagcat
     7021 ggcgccggcg ggcagctcct gaatgagtcg cgcccgctcg gccaccaccc gcacggcatc
     7081 ctccagggag aagacgccag ccaggcaggc cgcggtgtac tcgccgaggc tgtaaccgac
     7141 gagcgcgcgg ggcgacaggc cccactccag cagcagccga ctcaacgcgt agccgacggc
     7201 gaagaccgcc ggctgggcga tccgggtctc acgcaggtgc ccggcggagc cggctggatc
     7261 ggaggcacgg ccgaggagcc gccgcaggtc catgccgccg ccgctgcccc cctcgtcctc
     7321 cgcacggacg ccgccgggat agaggagctc gcgcagatcc tgccccagca ggggacggag
     7381 caactcggcg cagcggtcca gagcgtcacg gaacgtgggc tcctcacggt agagcccctc
     7441 gcccatgcgc gggtagtgct cgcccaggcc ggagagcatc cagaccagcg gcctctcgcc
     7501 cggcgcctgc actgccgaga ggagccgccg cggatcgcgc ccggcaaggc cggcgacggc
     7561 ctcctcgcgc gtccggcaga ccagggcccg ccggtgttgg aacgcgcggc gacccgcctg
     7621 gagggtaaac gcgatatcgg cgaggctctg ttcgggttgg gtctcgagat ggtcggcgag
     7681 ccggtccgtg gccgtctcca gcgccgtctc ggtgcgcgcg gagaacagca gaagctgcca
     7741 ggggcgggcg ggcgccggcg gcccaggatc gggcgcctct tccaggatgc aatgcacgtt
     7801 ggtgccgccc atgccgaagg agctgacgcc ggcgcgccgc
//
LOCUS       DASPJI010000324         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_17905, whole genome shotgun sequence.
ACCESSION   DASPJI010000324 DASPJI010000000
VERSION     DASPJI010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17905"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="VEL74_08615"
     CDS             complement(<1..1291)
                     /locus_tag="VEL74_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62630.1"
                     /translation="MNRIASLVLLIAAALLVAALPALAGVGAWTSLGPDGGPVRSLAV
                     DPANPRVVYAGTIHSGFYKSTDGGDTWSLTGRGLPAGDIMTIAFDPRRSSTLYVMVYG
                     TGVYRSTDAGRTWKVTSHQGRPFVQVIGLTVATDPRRPRVLYAGTRAGIYRSANGGAT
                     WKPRSRGLPPGSSVRALVIDPRTGTLYAGVQSHGVFRSDDQAATWQPADTGMPDGISV
                     PALALDPAGSGTILAGTDRGIYRSTDRGRTWQLTDAPEDIVLALAFQRRGRAYAGYSD
                     RGIAHSLDGGTTWQRPQEGPPPAAILGLAAGPEAVYAGTSEGREINGVFRSLDSGATW
                     GASQRNLTGLIVRGVAVDPTDPLTLYAGVELNGLVKSTDGGATWRPLDLPAGGGAIQQ
                     ILIHPLQPSTVYVSLTLNGPLLRSDDGGETWRRIGQSP"
     gene            complement(1326..3284)
                     /locus_tag="VEL74_08620"
     CDS             complement(1326..3284)
                     /locus_tag="VEL74_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62631.1"
                     /translation="MKRRSHLFLILAALALLALPTVPALAGAGLWTALGPDGGQVTAL
                     AVAPSDPDVVYAGTWTAGVFRSTDGGETWTFAGRGLGTSYVHAIATDPARPDTVYAST
                     TRGGVFKSIDGGATWAHVWDGGTPGGVTPVFALALDHRRPGVVYAGTNFGVFRSTDGG
                     VTWERRGNLSAGVSSLAVDSPGGWLFAGVNSMGVFKSGDQGKTWIPAGNGLPTSGAIQ
                     SLAIDPERPRTLLAATHLGIYRTHDRGRTWNPVVGALAAEVSAVAFQDGGRAYAAFRE
                     RGVYRSGNRGFTWERSIASLRDSLFLALAAGPVAVYTGDVNFSGSRGLVRSLDAGATW
                     EPSMRGLSNLGIRPIAADPSNPMRLYVGGFGLYQSTDRGATWTRVDFGLRPEPSAIHD
                     ILIDPARPARVYVSANDGPLLRSDDGGVTWERLNGGSVPLYLNDLAVDPRAPEALWGT
                     GSDGLGGGFFHSADGGETWQRVGGLPTEAFFTLYTAVDPRDPRVVYLGAAQNVDGVTE
                     PRLFRSADGGMTWERRDTGLADGNVWDIAFDAADPSILYALTSGVYRSTDAGLTWTLL
                     PARGRLFTSIATAPAGPGTPAVLCAARWDIVSPSAVMCSTDRGETWTALRRGLDTYSP
                     TELEVDPADPRRLYLGTLERGLMTYTLP"
     gene            complement(3399..>4084)
                     /locus_tag="VEL74_08625"
     CDS             complement(3399..>4084)
                     /locus_tag="VEL74_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62632.1"
                     /translation="LAFQAAAFDPRAPEALWAAGWQGVFHSSDGGRAWEQRTPDLGSS
                     FYFGEVQVDPSDPDIIYAAGGEVTSIRPVIFSPVVLRSADGGVTWERRDAGLEGGNVV
                     DFAVDAGDPSVLYAVTTRLFRSTDAGLSWTPAPGLGGFSYVTVATAAASSGTGPGAPA
                     AVYTAHQAPFGMVLRSTDQGETWRPVRFNLQPAIPQVLEVDPTNPAHLLLGTQNRSLL
                     SYTLGGGAP"
BASE COUNT          582 a         1377 c         1581 g          544 t
ORIGIN      
        1 ggggggactg gccgatgcgc cgccaggtct ctccaccgtc gtcgctgcgc agcagcggcc
       61 cgttgagggt gagcgacacg tagaccgtcg agggctgcag cgggtggatc aggatctgct
      121 ggatggcacc gccgcccgcc ggcaggtcca gtggccgcca ggtcgcgccg ccgtcggtgc
      181 tcttgaccag accgttgagc tccacgcccg cgtagagggt gagcggatcg gtgggatcga
      241 ccgccacccc gcgcacgatc aggccggtca agttgcgttg cgaggccccc caggtcgccc
      301 cggaatcgag gctgcggaag acgccgttga tctcccggcc ttctgaggtg ccggcgtaga
      361 ccgcctccgg tcccgccgcc aggccgagga tggcagccgg tgggggcccc tcctgcggcc
      421 gctgccaggt ggtgccgccg tcgaggctgt gcgcgatgcc gcggtcggag tacccggcat
      481 aggcccggcc gcgccgctgg aacgccagcg ccaggacgat gtcctcgggg gcgtcggtga
      541 gctgccaggt ccggccgcgg tcggtgctgc gatagatgcc cctgtcggtt ccggcgagga
      601 tcgtccccga gcccgccggg tcgagcgcca gggccgggac cgagatgccg tccggcatcc
      661 cggtgtccgc cggctgccag gtggccgcct ggtcatcgct gcggaagacg ccatgcgact
      721 ggacgccggc gtagagggtg ccggtccgcg ggtcgatgac cagcgcccgg accgagctgc
      781 ccggcggcag cccgcggctc cggggcttcc aggtggcgcc gccgttggcg ctgcggtaga
      841 tccccgcgcg ggtgccggca tagagcaccc gcggccggcg cgggtcggtg gccaccgtca
      901 ggccgatgac ctgcacgaac ggccggccct ggtgggaggt gaccttccag gtcctgccgg
      961 cgtcggtgct gcggtagacg ccggtgccgt agaccatcac gtacagcgtg ctcgagcgcc
     1021 gcggatcgaa ggcgatggtc atgatgtccc ccgccggcag gccgcggccg gtgagggacc
     1081 aggtgtcgcc accgtcggtg ctcttgtaga agcccgagtg gatggtgccg gcgtagacga
     1141 cgcgaggatt cgccggatcc acggccaggc tgcggaccgg gccgccgtcg gggccgagag
     1201 aggtccaggc gccgacgccg gccagggccg gaagcgccgc gacgaggagc gccgcggcga
     1261 tcagcaggac gagagaggcg atgcggttca tgacggtacc tcccgggtca atcatacctc
     1321 ctgcttcaag gaagggtgta ggtcatgagc ccgcgctcga gggtgccgag atagaggcgc
     1381 ctcgggtccg cggggtcgac ctccagttcg gtggggctgt aggtgtccaa cccgcggcgg
     1441 agcgcggtcc aggtttcgcc ccggtcggtg ctgcacatca cggcagaggg cgagacgatg
     1501 tcccagcggg ccgcgcagag cacggccggc gtccccggtc ccgccggggc ggtggcgatg
     1561 ctggtgaaga ggcggccccg ggcaggcagc agagtccagg tgagaccggc gtcggtgctg
     1621 cggtagacgc cggaggtcag ggcgtagagg atcgacggat cggcggcgtc gaaggcgatg
     1681 tcccagacgt tgccgtcggc caggccggtg tcgcggcgct cccaggtcat gccgccgtca
     1741 gcgctgcgga agaggcgcgg ctcggtgact ccgtcgacgt tctgcgcggc gcccaggtag
     1801 acgacgcggg ggtcgcgcgg gtccaccgcg gtgtacaggg tgaagaaggc ttccgtcggg
     1861 aggccgccga cgcgctgcca ggtctcgccg ccgtcggcgc tgtggaagaa gccgccgccg
     1921 aggccgtccg agccggtgcc ccagagcgct tcgggggcgc gggggtccac cgccaggtcg
     1981 ttcaggtaca ggggcacgct gccgccgttc agccgttccc aggtgacgcc gccgtcgtcg
     2041 ctccgcagca gggggccgtc gttggccgac acgtacaccc tggcggggcg cgccgggtcg
     2101 atcaggatgt cgtggatcgc ggacggctcc ggccgcaggc cgaagtcgac ccgcgtccag
     2161 gtggcgccgc ggtcggtgct ctggtacagg ccgaaaccgc ccacatagag cctcatggga
     2221 ttcgacgggt ccgccgcgat ggggcggatc cccaggttgg agaggccgcg catggacggc
     2281 tcccaggtcg cgccggcgtc caggctccgc accagcccgc ggcttcccga gaagttcacg
     2341 tcaccggtgt acaccgccac ggggccggca gccagggcga ggaagaggga gtcccgcaga
     2401 ctggcgatcg accgctccca ggtgaagccc cggttgccgc tccggtagac gccgcgctcc
     2461 cggaaagcgg cgtaggcgcg gccaccgtcc tggaaggcca ccgccgagac ttcggccgcc
     2521 agcgcgccga cgaccgggtt ccaggtccgg ccgcggtcgt gggtccggta gatccccagg
     2581 tgggtggcgg cgaggagggt gcgcggcctc tccgggtcga tggccagcga ctggatcgcc
     2641 ccactggtcg gcagtccgtt gcccgccggg atccaggtct tcccctggtc gccgctcttg
     2701 aagactccca tgctgttaac gccggcgaag agccagcctc ccggcgaatc cacggcaagc
     2761 gaggagactc cggccgaaag attgccgcgg cgctcccagg tgacgccgcc atcggtgctg
     2821 cggaagaccc cgaaattggt gccggcgtag acgacgccgg gccggcggtg gtcgagggcc
     2881 agcgcgaaga cgggggtgac gccgcccggc gtcccgccgt cccatacgtg cgcccaggtg
     2941 gcgccgccgt cgatgctctt gaacacaccc ccgcgggtgg tcgatgcgta gacggtgtcc
     3001 ggacgcgcgg gatcggtggc gatggcgtgg acatagctgg tgccgaggcc acggccggcg
     3061 aaggtccagg tctcgccgcc gtcggtgctc ctgaagacgc cggcggtcca ggtgccggca
     3121 tacacgacgt cagggtccga gggggccacg gcgagcgccg tcacctgccc gccgtcggga
     3181 ccgagcgcgg tccagaggcc ggcgccggcg agcgccggca cggtgggcaa ggccaggaga
     3241 gcgagcgcgg ccaggatcag aaagaggtgc gagcgacgtt tcatcgggat ccctccaagg
     3301 tataggtcat gaggctaccg tccgcggtgc cagataaaga tgcgccggat cgccgggatc
     3361 gccgtcaccg cgcctccgct ggctgcgcca ggagcgcttc agggcgctcc tcctcccagc
     3421 gtgtagctca ggaggctccg gttctgggtc cccagcagga gatgcgccgg attggtgggg
     3481 tccacctcca ggacctgggg gatggccggc tgcaggttga agcgcaccgg cctccaggtc
     3541 tctccctggt cggtgctgcg cagcaccatg ccgaaggggg cctggtgggc cgtatagacg
     3601 gcggccggag ctcctggccc ggtgcccgat gaggcagcgg cagtggcgac ggtcacgtag
     3661 gagaaccccc cgagcccggg cgccggcgtc catgagaggc cggcgtcggt gctgcggaag
     3721 agccgggtgg tgacggcgta gaggaccgaa ggatcgccgg cgtccacggc gaaatcgacg
     3781 acgttgcccc cttcgaggcc ggcgtcacgg cgctcccagg tgacgccgcc gtcggcgctc
     3841 cggaggacga cgggactgaa gatgaccggc cggatgctcg tgacctcgcc gccggcggca
     3901 tagatgatgt ccggatcgct cgggtccacc tggacctccc cgaagtagaa ggagctcccg
     3961 agatccggag tgcgttgctc ccaggcgcgg ccgccgtcgg agctgtggaa gacaccctgc
     4021 cagccagccg cccagagggc ctcgggagcc cgcggatcga aggcggcggc ctggaacgcg
     4081 aggc
//
LOCUS       DASPJI010000325         6402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_763871, whole genome shotgun sequence.
ACCESSION   DASPJI010000325 DASPJI010000000
VERSION     DASPJI010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763871"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="VEL74_08630"
     CDS             <1..1323
                     /locus_tag="VEL74_08630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYG62633.1"
                     /translation="RTVNSDLWLWPLGDKKAQPRNLTTSNLAEDTAPRYSPDGRFIAF
                     SRSRRPEIDPDFSRLALYDRASGEVRMLSPDWDVAEGDCVFTPDGRTLVFHVQERGRT
                     NIYALPVEGGKPRLLARGGTTGGVDVGPGANGGTRLVFQRESIAEPAELFSMDLTGSE
                     PQALTSFNAARMAELDQPTVDEATFEGAKGDPVHMFLVFPPGFDRARKWPLVQLVHGG
                     PYSSFTDSFGYRWTSALWASQGYVVAMVNFHGSTGYGQAFAESILGNHAELPYTDIMK
                     STDWLIAQGYIDEKRMAAAGGSYGGYLVSWILGQTDRFAAIINHAGVYDLMAQFASDA
                     TWGRPNNYGAAPWTDPKQVARFSPSYFAPNFKTPTLILHGEKDYRVPVTQGINLYGVL
                     QGKGVPTRIVLFPEENHWISKPQSSLLWWKEAFAWLDRWMGSGANGKS"
     gene            1436..2140
                     /locus_tag="VEL74_08635"
     CDS             1436..2140
                     /locus_tag="VEL74_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62634.1"
                     /translation="MKTARWGALTACLLIVTVAGPAVAQKVDERNDWSSVPLEGRLEP
                     VDTGSERAVAGMFSWEEVPLGYSGEISEDGGRATALVTDAEGRVLAEAVIDEKGSTVI
                     LAGVKITSEEEISDQESARVAEFVDTKEAAAIRALATHLAGLTPAEKRDKLLGITAVA
                     LTLGEGRGVPEVLLKAFGDCFGCCGPGCWGCYLAGNCYTGACAVHDGCVERLGHRHKT
                     CLKLLAVAVASYILQC"
     gene            complement(2190..5390)
                     /locus_tag="VEL74_08640"
     CDS             complement(2190..5390)
                     /locus_tag="VEL74_08640"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HYG62635.1"
                     /translation="MLRSARAPRLAVLLLLFTGLSPDAFAAEKAKPEARGFGSLQDPT
                     GIEALEYRLLGPAWGGRVSRAVGVPGDPRTYYAATASGGVWKSTDGGSSWNPIFDDQP
                     ISSIGSIAVAPSDPNVIYVGSGEANIRGNVAAGNGIYKSEDAGKTWSHVWRQEGQIGT
                     MAVHPSNPDVAFAAVLGHAFGPNPERGVYRTTDGGKTWRQVLKKDADTGASDVAIDPS
                     NPRTVFAGLWQARRRPWVMTSGGPGSGLYVSRDGGDTWTQLQEGLPKGIWGKVGVAVA
                     PSDGRRVYALIEAEEGGLFRSDDGGEAWSKVTGDRRLRQRAWYYTTLTVNPANPEELW
                     FPNVPLIKSIDGGKTLEMFMISHGDHHDVWLDPKDPKRIISAQDGGVDISTDGGETWL
                     SPALPIAQFYHVSVDNRVPFHVAGAMQDIGTGQAPSNTLRSDGPSNADWYRVGGGEAG
                     WVVPDPSAPDIVYAGEYLGYISRYDHRTRQARAVSAWPDNPSGFAGEQMRYRFQWTAP
                     IAVSPHDPKVVYHGANVLFRTGDGGQSWTPISPDLTRDDKSKQQWSGGPITGDNTGVE
                     TYCTIFAVAESPVERGLIWAGSDDGLVHVTRDGGKNWQNVTAAIPGLPEWGTVSMIEP
                     SRFDAGTAYLVVDAHRLDDMRPYLWKTADYGRTWKRLDGGLPKDIYLHAVREDPVKRG
                     MLYLGTERGVAVSRDDGATWRSLKLNLPTVAVHDLAVKDGSLVLATMGRSIWIFDHLA
                     ALRDFTPEVTAADVQLLPVADTIHWSYVRGPRLTGWRGQNPAQGASIYYWLKKEAEGD
                     VVLEILDGSGTVVNRLSSKPLGPTGSNEFVEDEEDVLSAFLASKKAGLQRAVWDGTWS
                     GAEMIKGAIVDFGFPLLGPRALPGTYTVRLTVGGQSRTAPLRLLPDPRNQVSEADRAE
                     QLRFALEVRDAITRLTRSVEQLRAVRRQLVARNELLAKDSSAEPLIRSSKDLLGKIDA
                     LEARFHNPKAEIAYDILAMKGGAALYSRLSPLFDFIKEGTGAPTQGSREVFAERKAEL
                     DRYEAEWNALRDGELAALNEQAKSQGMPAVYVPAAR"
     gene            complement(6159..>6402)
                     /locus_tag="VEL74_08645"
     CDS             complement(6159..>6402)
                     /locus_tag="VEL74_08645"
                     /inference="COORDINATES: protein motif:HMM:NF019275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HYG62636.1"
                     /translation="EKDLVRLYASIIVDRAGQKQIVVGRQGQMIKAIGTAARIDLEEY
                     LGRRVYLDLHVRVEENWRENRALLASMDRDVDLGIE"
BASE COUNT          968 a         2216 c         2205 g         1013 t
ORIGIN      
        1 cggaccgtca actccgacct gtggctctgg ccgttggggg acaagaaggc ccagccgcgc
       61 aacctcacca cctccaacct ggcggaggac accgcccccc gctactcgcc ggacggccgc
      121 ttcatcgcct tctcccgcag ccgccggccc gagatcgatc ccgacttctc gcgcctcgcg
      181 ctctatgacc gggcgagcgg cgaggtccgc atgctctccc cggattggga cgtggccgag
      241 ggcgattgcg tgttcacccc ggacggccgc accctcgtct tccatgtcca ggagcggggc
      301 cggacgaaca tctacgccct gccggtcgaa ggtgggaagc cgcgcctcct cgcccgcggc
      361 ggcaccaccg gcggcgtaga cgtcggtccc ggcgcgaacg gcggcacgcg gctggtcttc
      421 cagcgcgagt ccatcgccga gccggcggag ctgttcagca tggatctcac gggcagcgag
      481 ccccaggcgc tgacctcgtt caacgccgcc cgtatggcgg agctcgacca gccgacggtg
      541 gacgaggcga cgttcgaggg ggccaagggc gacccggtcc acatgttcct cgtcttccct
      601 ccgggcttcg accgcgcccg caaatggccg ctcgttcagc tcgtccatgg cggaccgtac
      661 agctccttca cggacagctt cggctaccgc tggacctcgg cgctctgggc gtcgcagggc
      721 tacgtcgtgg cgatggtgaa cttccacggc tccacgggtt acggtcaggc gttcgccgag
      781 tcgatcctgg gcaaccacgc cgagctgccc tacaccgaca tcatgaagtc gacggactgg
      841 ctgatcgccc agggctacat cgacgagaag cgcatggccg ccgccggcgg ctcctacggc
      901 ggctacctcg tctcctggat cctcggccag accgaccgct tcgccgcgat catcaaccac
      961 gccggcgtct atgacctgat ggcccagttc gcctccgacg ccacctgggg ccggccgaac
     1021 aactacggcg ccgccccgtg gaccgatccc aagcaggtgg cccgcttctc gccgagctac
     1081 ttcgccccca acttcaagac gccgacgctg atcctccacg gtgagaagga ctaccgcgtc
     1141 ccggtcactc aggggatcaa cctctacggc gtgctccagg gcaagggcgt ccccacccgc
     1201 atcgtcctct tccccgaaga aaaccactgg atctccaagc cccagtcctc gctcctctgg
     1261 tggaaggagg ctttcgcctg gctggaccgg tggatgggga gcggggccaa cggcaagagc
     1321 tgagatctcc ggggcttccg gccgcctacc cggcgcgccg ggagccccct ccgcctcccc
     1381 tgccgcacct ctctttgtag aaggacctcc aactcatcac tacaaagggg aaaacatgaa
     1441 gacagcacgt tggggagcac tgaccgcctg cctgctcatc gtgacggtgg ccggaccggc
     1501 cgtggcccag aaggtggacg agcggaacga ctggtcgtcc gtgcctctgg aaggacggct
     1561 ggagcccgtg gacaccggct ccgagagggc cgtcgcgggt atgttctcgt gggaggaggt
     1621 tcccctcggc tacagcggcg agatcagtga ggacggcggc cgcgcgaccg ctctcgtgac
     1681 cgacgccgag ggccgggtcc tcgccgaagc cgtcatcgac gagaagggct cgacggtgat
     1741 cctcgcgggc gtcaagatca ccagcgaaga ggagatcagc gaccaggagt cggcgcgggt
     1801 ggccgagttc gtcgacacca aggaagcggc cgccatccgc gctctggcca cccacctggc
     1861 cggcctgact ccggccgaga agcgggacaa gctcctcggg atcacggcgg tcgcgctgac
     1921 cctcggcgag ggacgcggcg tgccggaggt tctgctgaag gctttcggcg actgcttcgg
     1981 ctgctgtggt ccgggttgct ggggctgtta cctggcgggt aattgctaca ccggagcctg
     2041 cgccgtgcac gatggttgcg tcgaacgcct ggggcatcgc cacaagacgt gcttgaagct
     2101 cctcgccgta gccgtggcct cgtacatcct tcagtgctag cccgagagag cggcgaggaa
     2161 agccaaccct cctcgccgct cgcgcagccc taccgcgccg ccggcacgta caccgccggc
     2221 atcccctggc tcttcgcctg ctcgttcagc gccgccagct caccgtcgcg gagggcgttc
     2281 cactccgctt cgtagcggtc gagctcggcc ttgcgctcgg cgaagacctc gcggctgccc
     2341 tgggtaggcg cgccggtgcc ctccttgatg aagtcgaaga ggggcgagag acgagagtag
     2401 agggcggcgc cccccttcat cgccaggatg tcgtaggcga tctcggcctt ggggttgtgg
     2461 aagcgggcct ccagcgcgtc gatcttgccg agcaggtcct tggaggaacg gatcaggggc
     2521 tccgcgctgg agtccttggc cagcagctcg ttgcgcgcga cgagctggcg gcgcacggcg
     2581 cggagctgct ccaccgagcg cgtcagacgg gtgatggcgt cccgcacctc cagggcgaag
     2641 cggagctgct cggcgcggtc cgcctcggat acctggttcc gcgggtccgg caggaggcgc
     2701 agcggcgccg tccggctctg tccgcccacg gtcaggcgca cggtgtaggt gccgggcagg
     2761 gccctggggc cgagcagggg gaagccgaag tcgacgatcg ctcccttgat catctcggcg
     2821 cccgaccagg tgccgtccca caccgcccgc tgcagcccgg ccttcttgga cgcgaggaag
     2881 gctgacagga cgtcctcctc gtcctcgacg aactcgttgg agccggtggg ccccagcggt
     2941 ttgctcgaca gccggttgac cacggtgcca gagccgtcga ggatctccag gacgacatcc
     3001 ccttcggcct ccttcttgag ccagtagtag atggacgccc cctgtgccgg gttctgtccc
     3061 cgccagccgg tcagccgggg gccgcgcacg tacgaccagt ggatggtgtc cgccaccggc
     3121 aggagctgga catccgccgc ggtcacctcg ggggtgaagt cgcgcagcgc cgccaggtgg
     3181 tcgaagatcc agatcgaccg gcccatggtg gccaggacca ggctgccgtc tttgaccgcc
     3241 aggtcgtgca cggccacggt gggcaggttg agcttgaggg agcgccaggt ggcgccgtca
     3301 tcccgcgaga ccgcgacgcc ccgctcggtc cccaggtaga gcatcccccg cttcaccggg
     3361 tcctcgcgca cggcgtggag gtagatgtcc ttgggcaggc cgccgtccaa gcgcttccag
     3421 gtgcggccgt agtcggcggt cttccacagg taggggcgca tgtcgtccag ccggtgggcg
     3481 tccaccacga ggtaggcggt gccggcgtcg aaacgggagg gctcgatcat gctgaccgtc
     3541 ccccactcgg ggagcccggg gatggccgcg gtgacgttct gccagttctt gccgccgtcc
     3601 cgcgtcacgt gcaccaggcc gtcgtcgctc ccggcccaga tcagccccct ttcgaccggc
     3661 gactcggcca ccgcgaagat cgtgcaatag gtctcgacgc cggtgttgtc gccggtgatc
     3721 gggccgccgg accactgctg cttgctcttg tcgtcgcgcg tcaggtccgg gctgatgggg
     3781 gtccagctct ggccgccgtc gccggtgcgg aacagaacgt tggcgccgtg gtagaccacc
     3841 ttcgggtcgt gcggcgagac ggcgatcggg gcggtccact ggaaccggta acgcatctgc
     3901 tcgccggcga agcccgaggg gttgtccggc caggcgctca ccgcccgcgc ctggcgggtg
     3961 cggtggtcgt agcgcgagat gtagcccagg tactcgccgg cgtagacgat gtccggggcc
     4021 gacggatcgg ggaccaccca gcccgcctcg ccgccgccga cgcggtacca gtctgcgttg
     4081 gacggcccat cgctgcgcag cgtattgctc ggcgcctggc cggtgccgat gtcctgcatc
     4141 gccccggcga cgtggaacgg cacccggttg tcgacggaca cgtggtagaa ctgggcgatc
     4201 ggcagcgccg gcgacagcca ggtctcgccg ccgtcggtcg agatgtcgac gccgccgtcc
     4261 tgggcgctga tgatccgctt cgggtccttc ggatcgagcc agacgtcgtg gtggtcgccg
     4321 tggctgatca tgaacatctc gagggtcttg ccgccgtcga tgctcttgat gagcggcacg
     4381 ttggggaacc agagctcctc ggggttggcg gggttgacgg tgagggtcgt gtagtaccag
     4441 gcgcgctggc gcagccgccg gtcgccggtc accttgctcc aggcctcgcc gccgtcgtcg
     4501 gagcggaaga gccccccctc ttcggcctca atgagcgcat agacccgccg gccgtcggac
     4561 ggcgccaccg cgacgcctac cttgccccag atccctttcg ggagcccctc ctgcagttgc
     4621 gtccaggtgt cgccgccgtc ccgcgagaca tagaggccgc tacccggacc gccgctggtc
     4681 atcacccagg ggcgccgccg cgcctgccag aggccggcga agacggtgcg cggattgctc
     4741 ggatcgatcg ccacgtccga ggcgccggtg tcggcatcct tcttgagcac ctgccgccag
     4801 gtcttgccac cgtcggtcgt ccggtagacg ccgcgctcgg ggttgggccc gaaggcgtgc
     4861 cccagcaccg cggcgaaggc gacgtccggg ttggacgggt ggacggccat ggtgccgatc
     4921 tgcccctcct gcctccagac gtggctccag gtcttgcccg cgtcctccga cttgtagata
     4981 ccgttgcccg ccgccacgtt gccgcggatg ttcgcctcgc ccgacccgac gtagatcacg
     5041 ttggggtccg acggcgcgac cgcgatggag ccgatggacg agatgggctg gtcgtcgaag
     5101 atcgggttcc aggaggagcc gccgtcggtg gacttccaga cgccgccgga ggccgtggcc
     5161 gcgtagtagg tccggggatc gcccggcacg cccaccgccc gggagacgcg tccgccccat
     5221 gctggcccca ggagccggta ctcgagcgcc tcgatccctg tcgggtcctg gagggaaccg
     5281 aagcccctgg cctccggctt cgccttctcg gcggcgaagg cgtccggaga gaggccagtg
     5341 aaaagcagca gcaggacggc gagccgcggc gcgcgggcag atcgaagcat gggacctcct
     5401 cggtcggtcg gatgagacgg atctcagatg atctcaagcc gggagcgtag ccaagggaac
     5461 ggccgggagg caagcggagg gcgggtgtgt ggtgggcgcc ctactcaatc gccaggtcca
     5521 cgtcccggtc catggagaaa ctgtcgcaga aaacagaaga gtgtagcggc gcagcttacg
     5581 cgcccgctcg ctgcacgacg ccttgccgca gcgagcactc caaatgtcgc ggatgcagct
     5641 tcaacgagag atccgaagct tccggcgggc cgccggtaac tcagaaacgt cgctgcgtcg
     5701 gcaactctgt cgtggagaat ccataactgt cgcgtcgcgg aaccagaacg tcgcatcaag
     5761 actcgcactg tcgcacatca ggattcaagt gtctcgcagc tgtcctggta cgtcgcgcat
     5821 agactcagaa cgtcgcggag actcttccgg tgtcgcaacg tgtcgcgcgc tctcccgtcg
     5881 taaagtacga cgcgacaact gaaggttacg acgcgacatt gtcagcttag ccaggccgac
     5941 ggcggtcact ccgcgacact ctcggcctcg acaagccggc gccgggaaca ttgtcgcgtt
     6001 gttgtcgcac agtgtcgcgc gggacggtcc ggcgccgcgg cggaggttcc ggcggcgcag
     6061 ccgatgtgac gctacgaccc tcttccctcc ggcgaggcgg cggagagtac ggcgcgccgc
     6121 cggaacggac ggcgcgacag cggagtgtgg gggggggcct actcgatccc cagatccacg
     6181 tcccggtcca tcgaggcgag gagagcgcgg ttctcgcgcc agttctcctc gacgcggacg
     6241 tggaggtcca ggtagacgcg gcggcccagg tactcttcca ggtcgatgcg ggcggcggtg
     6301 ccgatcgcct tgatcatctg gccctggcgg ccgacgacga tctgcttctg gccggcacgg
     6361 tcgacgatga tggaggcgta gaggcgcacc aggtccttct cc
//
LOCUS       DASPJI010000326         3693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_362934, whole genome shotgun sequence.
ACCESSION   DASPJI010000326 DASPJI010000000
VERSION     DASPJI010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362934"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(359..538)
                     /locus_tag="VEL74_08650"
     CDS             complement(359..538)
                     /locus_tag="VEL74_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62637.1"
                     /translation="MKAKLIFMLALVALTLSLQPVQSQTSCSAFCSKVRCIGGYICGP
                     YVNSSGQTVCGCHPR"
     gene            complement(623..3040)
                     /locus_tag="VEL74_08655"
     CDS             complement(623..3040)
                     /locus_tag="VEL74_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HYG62638.1"
                     /translation="MNRYVCVHGHFYQPPRENPWLEAIEIQDSAYPYHDWNERVTAEC
                     YAPNAASRIIDREGRIERIVNNYSRISYDFGPTLLAWLETARPKVYRAIITSDRLSAE
                     RFSGHGSALAQPYHHTILPLSNARDKRTQILWGLADFEHRFARKAEGVWLPEAAVDLE
                     SLDILAAQGVRFTLLEPHQAKRVRPKGAKEWHDVSDGRIDTTQPYEAVLPSGRRMAIF
                     FYDGPASRAIAFEGLLSSGERFADRILAGAPRDSGRDRLLHVATDGETYGHHHRYGEM
                     ALTYALHHIESHRLARLTNYGEYLESHPPEHEVEILENTSWSCVHGVGRWHDDCGCHT
                     GGEHGWNQAWRSPLRKALDWLRDELAPLYEREAKRWLADPWAARDGYVSVVLDHSPEN
                     MEAFLRRHAAGELPAGEDRVRVLKLLEMQRHAMLMYTSCGWFFNDLAGIETVQILRYA
                     GRAVQIAGNGVEAEFLRRLERARSNSPAAGSGRDLYEKHVRPAMIDLPQVAAHYAVTS
                     LFRDSPNPDRVYCYSVERESGRVFRAGRSRLALGKVRITSDLTGKSARLSFGVVHFGD
                     HNLSAGVRDTDDNGAYDHLVEEAGGAFEHGDLAQVVRLLDRYFGDVPYSLKSLFRDEQ
                     RRVLDLVLASTLEEAEDEFRDVYRHHAPLMRFLESLDNPLPNAFRAAAELVLNSDLRR
                     SIGEVPMDLEEIRRLLAEARSLGVELDVKGLSYELEKALEAEVERLHESPVEPGLLER
                     VEGAVSLARSAPFQLDLWQVQNRCYEMLQAVYPDKRSEAEAGDEAGEEWVRRFRALAE
                     ELRIQVP"
     gene            complement(3037..>3693)
                     /locus_tag="VEL74_08660"
     CDS             complement(3037..>3693)
                     /locus_tag="VEL74_08660"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HYG62639.1"
                     /translation="RMLGERIASLVPFEAQKLAAGAVILSPFIPLLFMGEEWGETAPF
                     LYFISHTDEDLVGAVQEGRREEFAGFGWQEEPPDPQSPETFERSRPDPGLREREPNRQ
                     LYGFYRELLRLRKELPALARPSKRRIETGFDEDARVVWARRWNGDGAEAFLVFAFGEG
                     EASPTLPLPAGRWTKVLDSAEERWGGPGSDAPAEAESGGEVQVTVAGRSVVLYHTPLE
                     "
BASE COUNT          561 a         1282 c         1282 g          568 t
ORIGIN      
        1 aaggtacacg ttctccggtg ctaccatcgc gatggttgcg cattggacta acactccttg
       61 cgtaactagg gctcgagtcg agggctcaac tcactcgggc ccgctccttg tttctggccg
      121 gacacacgcc ggccgcttca cttcacaagt ggggattgct gggcttcgtc tctctccgtc
      181 tcacctcctc cccggcgacg aacgaatgcg gcgggttaca tggcggagcg taacaaaggc
      241 gccgggcgaa agcaatgctc cgggagcgat ccgaaaagat ttcttctggg ggagaccgcg
      301 ggaagctcct tcgggctcag cacccgggaa gcttcccgca ccgttcagag agggcgtttc
      361 agcgcgggtg gcaaccgcag accgtctggc ccgaggagtt gacgtagggt ccgcagatgt
      421 agccgccgat gcagcggacc ttggagcaga acgcggagca ggaggtctgc gactggaccg
      481 gctggagcga gagggtgagc gcgacgagcg cgagcatgaa gatcagcttg gctttcatgg
      541 gggttctcct tcgggtctga gagggcaagt cctctcgcaa ctaccaggtg cgccggccat
      601 ggctcgccga gcccggccgg ccctacggca cctggatgcg cagctcctcg gccagcgcgc
      661 ggaagcggcg gacccactcc tcccccgcct cgtcccccgc ttcggcctcg ctgcgcttgt
      721 ccgggtagac ggcttggagc atctcgtagc accggttctg cacctgccag aggtcgagct
      781 ggaaaggggc ggagcgggcg agggagaccg ccccctccac gcgctccagc aggccgggct
      841 cgacggggct ctcgtgcaga cgctccacct cggcctcgag cgccttttcc agctcatagc
      901 tcaacccctt gacgtccagc tccaccccca ggctccgggc ttcggccagg aggcgccgga
      961 tctcctcgag gtccatcgga acctcgccga tggaccgccg aaggtccgag ttgagcacca
     1021 gctcggccgc ggcgcggaag gcgttcggga gcgggttgtc caggctctcg aggaagcgca
     1081 tgagcggcgc gtgatgacgg tagacgtcgc ggaactcgtc ctcggcctcc tccagcgtcg
     1141 aggcgaggac caggtccagc acccggcgct gctcgtcacg gaacagcgac ttcaacgagt
     1201 aggggacgtc gccgaagtag cggtcgagga gacggacgac ctgcgcgagg tcgccgtgct
     1261 cgaaggcccc gcccgcctcc tccaccagat ggtcgtaggc accgttgtcg tcggtgtccc
     1321 gcacgccggc gctcaggttg tggtcgccga agtggaccac cccgaagctc agacgtgccg
     1381 acttgccggt gagatcggag gtgatcctca ccttccccag ggccagacgg gagcggccgg
     1441 cccggaagac ccgcccgctc tcccgctcca ccgagtaaca gtagacccgg tccgggttcg
     1501 gcgagtcccg gaacagcgag gtcaccgcgt agtgggccgc cacctggggg aggtcgatca
     1561 tcgccgggcg gacgtgcttc tcgtagaggt cccgcccact gccggccgcg ggcgaattgc
     1621 tgcgcgcccg ctccaggcgc cgcaggaatt cggcctcgac gccgttgccg gcgatctgca
     1681 cggcgcggcc ggcgtagcgc aggatctgga cggtctcgat ccccgcgagg tcattgaaga
     1741 accagccgca gctcgtgtac atgagcatgg cgtggcgctg catctccagc agcttgagga
     1801 cccgcacccg gtcctccccg gccggcagct ccccggccgc gtgccggcgc aggaaggcct
     1861 ccatgttctc gggcgaatgg tcgagcacca ccgacacgta gccgtcgcgg gcggcccacg
     1921 ggtcggcaag ccagcgcttc gcctccctct catagagcgg ggcgagctcg tcgcgcagcc
     1981 agtcgagcgc cttgcgcagc ggcgaccgcc aggcctggtt ccagccgtgc tctccgccgg
     2041 tgtggcagcc gcagtcgtcg tgccaccgcc ccaccccatg gacgcagctc caggaggtgt
     2101 tctccaggat ctccacctcg tgctccggcg gatggctctc caggtactcg ccgtagttgg
     2161 tgagccgggc caggcggtgg gactcgatgt ggtgcagcgc gtaggtgagc gccatctcgc
     2221 cgtagcggtg gtggtgccca taggtctcgc cgtcggtcgc cacgtgcagc agccggtcgc
     2281 ggccggagtc ccgcggcgca ccggccagga tgcggtcggc gaagcgctcg cccgaggaga
     2341 gcagcccctc gaaggcgatg gccctcgacg ccgggccgtc atagaagaag atggccatcc
     2401 ggcgccccga aggcagcacg gcctcgtagg gctgggtggt gtcaatgcgc ccatcggaga
     2461 cgtcgtgcca ctccttggcg cccttgggcc ggacccgctt cgcctggtgc ggctcgagga
     2521 gggtgaagcg caccccctgc gccgccagga tgtccaggct ctccaggtcg accgccgcct
     2581 ccggcagcca gaccccctcg gccttgcggg cgaagcggtg ctcgaagtcg gccaggcccc
     2641 agaggatctg ggtccgcttg tcgcgcgcat tggagagcgg caggatggtg tggtggtacg
     2701 gctgggcgag ggccgagccg tggcccgaga agcgctcggc gctcagccgg tcggaggtga
     2761 tgatcgcccg gtagaccttg ggccgcgccg tctccagcca ggcgaggagg gtcgggccga
     2821 agtcgtagct gatgcggctg tagttgttga cgatccgctc gatccggccc tcgcggtcga
     2881 tgatccggga ggcggcgttg ggtgcgtagc actcggccgt gacccgctcg ttccagtcgt
     2941 ggtacgggta ggccgagtcc tggatctcga tggcttcgag ccacgggttc tcccgcggcg
     3001 gctgatagaa gtggccgtgg acgcagacgt atcgattcat tcgaggggcg tatggtacag
     3061 gaccaccgaa cgccccgcca cggtcacctg gacctcgccc ccggattcgg cctccgcggg
     3121 agcgtcgctc cccggaccgc cccaccgctc ctccgcggaa tccagcacct tcgtccagcg
     3181 ccccgccggc agcggcaggg tgggagacgc ctccccttca ccgaacgcga agaccaggaa
     3241 ggcctccgca ccgtccccgt tccagcgccg tgcccagacc acccgcgcgt cctcgtcgaa
     3301 gccggtctcg atccgccgct tgctgggccg ggcgagggcc ggcagctcct tgcgcagccg
     3361 gagcagctcc cggtagaagc cgtagagctg ccggttgggc tcccgctcgc gcaggccggg
     3421 atcgggacgg gagcgctcga aggtctccgg cgactgcgga tcgggcggct cctcctgcca
     3481 gccgaagccg gcgaactctt cccgccgccc ctcctggacg gcgcccacca gatcctcgtc
     3541 cgtgtggctg atgaaataga ggaagggggc ggtctccccc cactcctcgc ccatgaagag
     3601 gagagggatg aacggcgaca ggatcaccgc gccggcggcg agcttctgcg cctcgaaggg
     3661 gacgagggac gcgatccgct cccccagcat gcg
//
LOCUS       DASPJI010000327         3317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_313703, whole genome shotgun sequence.
ACCESSION   DASPJI010000327 DASPJI010000000
VERSION     DASPJI010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313703"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VEL74_08665"
     CDS             complement(<1..803)
                     /locus_tag="VEL74_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYG62640.1"
                     /translation="MPAARCFQLLVLNTLLLVGALLEIRLAWAAVALDLLVVAAALID
                     HRRAGRVPLEAARRWPPLLVQGAAAEVDLTLTVGRDVADRSLTLRLREGLHPALAESP
                     LRHEVILAPDADLRWRYGLVPRRRGEHALAPLTVRVLGPWKLAWAQRDLLPAETVRVY
                     PQVRWEGRVGQLLALAQRHRMGQVAMRLHGVGTEPYALREYLPGDPLGKIHWKATARH
                     GRLVSREDTWERSARLVILLDCARTMASMDGHRSKLDYALAAALALTRVA"
     gene            complement(805..1809)
                     /locus_tag="VEL74_08670"
     CDS             complement(805..1809)
                     /locus_tag="VEL74_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYG62641.1"
                     /translation="MIQNPHTAAETLSVAAHRIADALDGVVLGQRGAVETLLTAYMAG
                     GHALLEGVPGLGKTLLARAFASCLGVPFSRIQFTPDLMPADVIGTNVFDGASGAFRLI
                     RGPVFTQVLMADEINRTPPKTQSALLEAMQEGQVTIDGAGHPLSPGFFVIATQNPLEF
                     EGVYPLPEAQLDRFLVRIEMGLPPREAELELYRKAVAGEVAGGFLGAGPGAAALPEPV
                     VAPGEAFALRGASRQVHVSAELLDYLARLAEASRRSPQVELGVSPRAALALLEAGRAA
                     ALLEGRSFVLPEDFKRLLVPCWAHRLILTPESELEGHTPRQELEQVAAAVEVPHERRE
                     "
     gene            complement(1806..2939)
                     /locus_tag="VEL74_08675"
     CDS             complement(1806..2939)
                     /locus_tag="VEL74_08675"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HYG62642.1"
                     /translation="MSQVRKWGLFLLVSLLALIALVFTSGPGAARGSVLSQGAGGWLA
                     ARRYLEAREAQVKLLDRPLDQLQDKGVLVVAFPWQVGTFGDPGEALTQHLRRGGDLVL
                     AYSGGLSSLAELLAFDALTGDLEEIRTPPLQPARWRRFVHEEWTLRPAPAKSATEEWT
                     AARPLRVWAPRAIPEPPKEAQVLFQGPVGEAAVFAFRRYGGRVVVLPADLFSNARLAD
                     EGNADLLETLLRDLGPSWTFDEYHHGLARQGEIEGPVSGPVLDLLLAHLAVLYLLAVW
                     ALARRFGPAWREVPAIAGSTASFLLGLGALHHRLGHHRDAALLLVRRTGELHRDLAVP
                     EELTRRAQAPEVMDADGLVSIARSLVRLRRGRPPVPDPTENRP"
     gene            complement(2936..>3317)
                     /locus_tag="VEL74_08680"
     CDS             complement(2936..>3317)
                     /locus_tag="VEL74_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62643.1"
                     /translation="YKVLLPWWRARRDRRQSKKGTGEAVPSTLPAAAAGMDAAAWRAE
                     LDRCLAEGRVADGLHAAWWWLARSVAGARAEPTWTGRDLLAQTRRQDLRELVRRLDIL
                     LYGPQRPELDDLRRLVGRMEETLA"
BASE COUNT          474 a         1247 c         1222 g          374 t
ORIGIN      
        1 gccacccggg tgagggcgag cgccgccgcc agggcgtagt ccagcttgct gcggtggccg
       61 tccatggagg ccatggtgcg ggcacagtcg aggaggatca ccagccgggc gctgcgctcc
      121 caggtgtcct cgcggctcac cagtcggcca tggcgcgccg tcgccttcca gtggatcttc
      181 cccagcggat cgcccggcag gtactcgcgc agggcatagg gctcggtgcc cacgccgtgg
      241 agccgcatcg ccacctgccc catgcggtga cgctgcgcga gggcgaggag ctgccccacc
      301 cttccctccc agcggacctg cggatagacc cgcaccgtct ccgccggcag gaggtcgcgc
      361 tgcgcccagg cgagcttcca ggggcccagc acccgcacgg tcagcggcgc cagggcgtgc
      421 tcgccgcggc ggcgcggcac caggccgtag cgccagcgga gatccgcgtc cggcgcgagg
      481 atcacctcgt ggcgcagcgg gctctcagcc agcgccggat ggagaccctc gcgcagacgg
      541 agggtgagac tccggtccgc gacgtcccga ccaacggtga gcgtcagatc cacctcggcg
      601 gcggcgccct gcaccaggag agggggccag cggcgcgccg cctccagggg gacgcggccg
      661 gcacgccggt gatcgatcag cgccgcggcg acgaccagca ggtcgagggc cacggcggcc
      721 caggcgaggc ggatctccag cagcgcccct accaggagga gcgtgttgag gaccaggagc
      781 tggaagcagc gggcggccgg catctcactc ccggcgctcg tgcgggacct cgaccgccgc
      841 cgccacctgc tccagctcct ggcgcggcgt gtgcccctcc agctccgact ccggcgtgag
      901 gatcaggcgg tgggcccagc aggggaccag caggcgcttg aagtcttccg gcagcacgaa
      961 gctccggcct tcgagcagcg ccgccgcccg gcccgcctcg aggagcgcca gcgccgcccg
     1021 cgggctcacc cccagctcca cctgcggcga acggcgcgac gcctccgcca gccgcgccag
     1081 gtagtccagc agctccgccg agacgtgcac ctgccgcgag gcgccacgca gggcgaacgc
     1141 ctcgccgggg gcgaccaccg gctccggcag ggccgccgct ccgggcccgg cgccgaggaa
     1201 accaccggcg acctcgcccg ccaccgcctt gcggtagagc tccagctccg cctcgcgcgg
     1261 cggcagcccc atctcgatcc gcaccaggaa gcggtcgagc tgcgcctcgg gaagcgggta
     1321 gaccccctcg aactccagcg ggttctgggt ggcgatgacg aagaagccgg gcgacagcgg
     1381 gtgcccggcg ccgtcgatcg tgacctgccc ctcctgcatc gcctcgagca gcgccgattg
     1441 ggtcttgggc ggcgtgcggt tgatctcgtc ggccatcagc acctgggtga agaccgggcc
     1501 gcggatcagc cggaaggcgc cggaggcacc gtcgaagacg ttggtgccga tgacgtcggc
     1561 gggcatcagg tcgggggtga actggatgcg gctgaacggc actcccaggc aggaggcgaa
     1621 ggcgcgggcg agcagggtct tgcccagccc gggcaccccc tcgaggaggg cgtggccgcc
     1681 ggccatatag gcggtcagta gggtctccac cgccccccgc tggccgagga cgacgccgtc
     1741 cagggcatcg gcgatgcggt gcgccgccac ggatagggtc tcggcggcgg tgtggggatt
     1801 ctggatcatg gtcggttctc cgtgggatcg ggcacagggg ggcggccgcg ccgcagccgg
     1861 acgagggagc gggcaatgga caccaggccg tcggcatcca tgacctcagg ggcctgggcg
     1921 cgccgcgtca gctcctccgg caccgccagg tcgcggtgca gctccccagt gcggcggacc
     1981 agcaggagcg ccgcgtcgcg gtggtggccg agccggtggt gcagcgcccc cagacccagc
     2041 aggaaggagg ccgtggaacc ggcgatggcc ggcacctcgc gccaggcggg gccgaagcgg
     2101 cgggccagcg cccagaccgc caacaggtag aggacggcca ggtgcgccag cagcaggtcc
     2161 agcaccggcc cggacaccgg gccctcgatc tcgccctgcc gggccagccc gtggtggtac
     2221 tcgtcgaagg tccagctcgg gcccaggtcc cgcaggaggg tctccaggag gtccgcgttc
     2281 ccctcgtccg ccaggcgcgc gttcgagaag aggtcggcgg gcaacaccac tacccggccg
     2341 ccgtagcgcc ggaaggcgaa gaccgcggcc tcccctaccg gcccttgaaa aagcacctgc
     2401 gcctccttgg ggggctccgg tatcgcccgc ggggcccaga cccgcaacgg ccgcgccgcc
     2461 gtccactcct ccgtggccga tttcgccggc gccgggcgca gggtccactc ctcgtgcacg
     2521 aagcggcgcc aacgcgcggg ctggaggggc ggggtgcgga tctcctccag atcaccggtc
     2581 agggcgtcga acgccaggag ctcggcgagg ctggagagcc cgccggagta ggccaggacc
     2641 aggtccccgc cgcgccgcag atgctgggtc agcgcctcgc cgggatcgcc gaacgtcccc
     2701 acctgccagg ggaaggccac caccagcacc cccttgtcct ggagctgatc gagcgggcgg
     2761 tcgaggagct tcacctgggc ctcccgcgcc tccaggtagc ggcgggcggc gagccagccg
     2821 ccggccccct gggacaggac gctcccccgc gcggcccccg ggccgctggt gaagacgagg
     2881 gcgatcaagg ccaggaggct caccagcagg aagagccccc acttccggac ttggctcacg
     2941 ccagggtctc ctccatccgt cccaccagcc gccgcaggtc gtcgagctcc ggccgctgcg
     3001 gcccatagag aaggatatcg agccgccgca ccagctcccg cagatcctgg cggcgtgtct
     3061 gggccaacag gtcgcggccg gtccaggtcg gctctgcgcg cgccccggcc accgaccggg
     3121 cgagccacca ccaggccgca tggagcccgt cggcgacccg cccctcggcc aggcagcggt
     3181 cgagctcggc gcgccaggcg gcggcgtcca tcccggcggc ggcggccggg agtgtggacg
     3241 gcacggcctc gcccgtcccc tttttactct gccggcggtc ccggcgtgcc cgccaccagg
     3301 ggaggaggac cttgtac
//
LOCUS       DASPJI010000328         3194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_591222, whole genome shotgun sequence.
ACCESSION   DASPJI010000328 DASPJI010000000
VERSION     DASPJI010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591222"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="VEL74_08685"
     CDS             complement(<1..1365)
                     /locus_tag="VEL74_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HYG62644.1"
                     /translation="MEPNRGSVPSSPAEAPAPTSPGPRRRPRKWLRRIGKGLVFLLGL
                     VLVLAAASGLWLRSKLRASLPELEGERRVEGLAAPATVERDAAGIPTIQGASRRDVAF
                     AEGYVHAQDRLFQMDLMRRQSAGELAELFGPGVVQADRDVRVHRFRARAARLLQESPP
                     QVREILEAYAQGVNAGRASLGGDPFEYLAMRAKPSPWRAEDSFLVLFSMFMQLNDEEG
                     MTERTLSQLREAVPPALFEFLTPQGTAWDSPLVGDRLPSPPVPGPQVCNVPARPAPAA
                     EPKAASLTPPAPREVPGSNGWVVAGSHTADGGALLANELHLGLMVPNLWYRVSLSWPD
                     GAGGRHRVTGVTLPGAPAMVLGSNGRVAWGVTNSAIDTTDLITLEVDPARPDTYRTPE
                     GPRRFERHREVIRVKGGEEEAVDVDSTVWGPVSGKDWKGRPQVLRWTAYEPGAADFQI
                     LGLET"
     gene            complement(1376..>3194)
                     /locus_tag="VEL74_08690"
     CDS             complement(1376..>3194)
                     /locus_tag="VEL74_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HYG62645.1"
                     /translation="QMNGWGHYTPARLKPGISIERAQEEMTALVRRLAGGNPPVGMTQ
                     GVVLGPLREELIRDLPRLLMMLLAAAGLLLLIACVNVSNLLLAQSLNQSTEVAVRVAL
                     GATRGRLLRQFFTYSVVLALLGGLVGLLLTFWSVKPLVALASVQQAVNEFDIEPRIDL
                     PTLGFTFLTSLVVGLLFGLVPALRVSKANVNHTLKEGGRSGSLGVAGQRVLSTFVVAE
                     VALALVLLVGAALTFQSMRRVVQEDRGFDMENVLSFEVALPEQKFPEPLRKMDFIRQS
                     LERLRGIPGVQAAGATTTQPLYPGTYTLPFNPEGKPANNPMGAYFTHDRVVTPDYMKS
                     LGIPLLRGRGITEQDTAETPMVAVISQSMAERYWPGEEAIGKRLKPGPYDSPAPWVTV
                     VGIAGNLKETFDPGMPNLEHDAWYRPYQQAAPFFESIIFTLKTQGDTRSIAGAARQAV
                     AEVDRDLPVYDLMTMEEHLAERTTQERFSAWLYGTLGALGLLLAALGIYGILSFSVNQ
                     RLREIGIRSAMGAAPADVRALVLRKALGLTALGLGLGLVAVLFLTRFLSFQLYQISNN
                     DPVTLALALIVLGLIALVSSYIPASRAARVDPVRALRYQ"
BASE COUNT          480 a         1165 c         1115 g          434 t
ORIGIN      
        1 ggtctccagg ccgaggatct ggaagtccgc cgctccgggc tcgtaggcgg tccagcgcag
       61 gacctggggg cgccccttcc agtccttgcc gctgacggga ccccagaccg tggagtccac
      121 gtccaccgcc tcctcttctc ctcccttgac ccggatcacc tcccggtgac gctcgaagcg
      181 ccgcgggccc tccggagtgc ggtaggtgtc gggacgggcg gggtccacct ccagggtgat
      241 cagatcggtg gtgtcgatgg cgctgttggt caccccccag gcgacacggc cgttgctgcc
      301 cagcaccatg gccggagcgc cgggcagcgt cacaccggtc acccggtgcc gccccccggc
      361 gccgtccggc cagctcagcg agacgcggta ccagagattg ggcaccatca gccccaggtg
      421 cagctcgttg gccagcaggg cgccgccatc ggcggtgtgg ctgccagcca ccacccagcc
      481 gttgctcccc ggcacctccc tgggtgccgg gggcgtcagg gaggcggcct tgggctcggc
      541 cgcgggcgcc gggcgcgccg gaacgttgca cacctgcggg ccgggcaccg gcggcgacgg
      601 cagccggtcg cccaccaggg gagagtccca cgccgtaccc tgcggcgtga ggaactcgaa
      661 cagggcgggc ggcaccgcct cccgcaattg cgagagcgtc cgctccgtca tcccctcctc
      721 gtcgttgagc tgcatgaaca tgctgaagag gacgagaaag ctgtcctccg cccgccacgg
      781 ggacggcttg gcccgcatcg ccaggtactc gaaaggatcg cctcccaggg aagcgcggcc
      841 ggcgttcacc ccctgggcat aggcctccag gatctcccgc acctggggag ggctctcctg
      901 gagcaggcgc gccgcccggg cgcggaagcg gtggacgcgc acgtcccggt ccgcctgcac
      961 caccccgggg ccgaacagct cggcgagctc gccggccgac tggcgccgca tcaagtccat
     1021 ctggaacagg cggtcctggg cgtggacata cccctcggca aaggcgacgt cgcggcggct
     1081 cgccccctgg atggtgggga tgcccgccgc gtcccgctcc accgtggcgg gagcggccag
     1141 gccctccacc cgccgctccc cctccagctc cggcaggctc gcgcgcagct tggagcgtag
     1201 ccagaggccg ctcgcggcgg cgaggaccag caccaggccg agcaggaaga ccaggccctt
     1261 gccgatccgc cgcagccact tgcgcgggcg gcgccgaggg ccggggctcg taggtgccgg
     1321 cgcctcggcc ggtgaagatg ggacgctgcc gcgattcggc tccatgtcgc ctccgctact
     1381 gataacgcaa ggcccgcacc gggtcgactc gcgccgcccg gctggccggg atatagctgg
     1441 acaccagcgc gatcaggccc agcacgatga gggcgagcgc cagcgtcacc gggtcgttgt
     1501 tgctgatctg atagagctgg aaggagagga aacgggtgag gaacagcacc gccacgagcc
     1561 ccaggcccag gccgagcgcc gtgagcccca gcgccttgcg cagcaccagc gcccgcacgt
     1621 ccgccggggc ggcgcccatg gcggagcgga tgccgatctc gcgcagccgc tggttgaccg
     1681 agaacgacag gatgccgtag atgcccagcg ccgccagcag caggccgagc gcgcccagcg
     1741 tgccgtagag ccaggcgctg aagcgctcct gtgtcgtgcg ctcggccagg tgctcctcca
     1801 tggtcatcag gtcgtagacc ggcaggtccc ggtccacctc ggccaccgcc tggcgcgccg
     1861 cgcccgcgat gctccgggtg tcaccctggg tcttcagggt gaagatgatc gactcgaaga
     1921 acggcgccgc ctgctgatag gggcggtacc aggcgtcgtg ctccaggttc ggcatccccg
     1981 gatcgaaggt ctccttgagg ttgccagcga tgcccaccac cgtgacccac ggcgccgggc
     2041 tgtcgtacgg ccccggcttc agccgtttgc cgatcgcttc ctccccgggc cagtagcgct
     2101 ccgccatcga ctggctgatg accgccacca tcggtgtctc cgccgtgtcc tgctcggtga
     2161 tcccccgccc cctgagcagc gggatgccga gcgacttcat gtagtccggc gtgaccaccc
     2221 ggtcgtgggt gaagtaggcg cccatcgggt tgttggccgg cttgccctcg gggttgaacg
     2281 gcagcgtgta ggtgcctgga tagagcggct gcgtagtggt ggcaccggcc gcctgtacgc
     2341 cggggatgcc ccgcaggcgc tccaggctct ggcggatgaa gtccatcttg cgcagcggtt
     2401 cggggaactt ctgctcgggc agagccacct cgaaggagag gacgttctcc atatcgaagc
     2461 cgcggtcctc ctgaaccacc cgccgcatgc tctggaaggt gagcgccgcg ccgaccagga
     2521 gcacgagcgc cagcgccacc tcggccacca cgaaggtgga gagcacccgc tggccggcga
     2581 cgccgaggct gccggaacgc cccccctcct tgagcgtatg gttgacgttg gccttggaga
     2641 cccgcagcgc cggcaccagg ccgaagagga gccccaccac cagcgaggtc aggaaggtga
     2701 agcccagagt gggcaggtcg atccgcggct cgatgtcgaa ctcgttgacc gcctgctgca
     2761 ccgaggccag ggccaccagc ggcttgacgc tccagaaggt cagcagcagc cccaccaagc
     2821 cgccgagcag cgccagcacc acgctgtagg tgaagaactg ccgcagcagg cggccgcggg
     2881 tggccccgag ggcgacccgc accgccacct cggtgctctg gttcaggctc tgcgcgagca
     2941 gcaggttgga gacgttgacg caggcgatga gcagcagcag gccggcggcg gcgagcagca
     3001 tcatcagcag gcgcggcagg tcgcggatca gctcctcgcg caacgggccc agcaccaccc
     3061 cctgggtcat gcccaccggc gggttgcccc ccgcgaggcg ccgcaccagg gccgtcatct
     3121 cctcctgcgc ccgctcgatg gagatccccg gcttgaggcg ggcgggcgtg tagtggcccc
     3181 agccgttcat ctgc
//
LOCUS       DASPJI010000329         5795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_362969, whole genome shotgun sequence.
ACCESSION   DASPJI010000329 DASPJI010000000
VERSION     DASPJI010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362969"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="VEL74_08695"
     CDS             complement(<1..1397)
                     /locus_tag="VEL74_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62646.1"
                     /translation="MIDLNAPVRTVAGVDLALAEGQADSKPLAYYVLPPPPRVAVAEG
                     VPQVELLRFVRDGELAGGHLRLEVELAHPPARLDEVREALAAELREEEPAIELRPLLA
                     EVATAELLFAGLDGDGEGGLATLIRRGFGRAPAGFAPPYRAAFSVDLDPDGARLVEAA
                     LRSGGAPIGIIYRLAVEGLWPAQRVVARVDWSRVYDHFSDHHKEGSFLLVTDVQRLAE
                     RLIENRVIEIQVIQGLVPEEGAPQPDPGAALAWVQRELVERFCEPVMPLDHEPAHASL
                     GTMGEIFGVGTAYAVKSLTQIERAVAEVDFQRAIVVSRTLTPQAHLADLLGGASPDQH
                     IADAGLDHPFFRRFALRIGTARPLAELGLSEMVLHVEYGTASESLRLTPGSPEARFES
                     WSDASPDGSWTLRSEVTFDAASPLGSLEGGGRAMLPPLSGTSRVLFMDLERLLGIARY
                     EVRGTTDSRVLLTQAT"
     gene            complement(1410..3722)
                     /locus_tag="VEL74_08700"
     CDS             complement(1410..3722)
                     /locus_tag="VEL74_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62647.1"
                     /translation="MLTMTNPLTIKGFTVYHDDADEIQALQSGGPPSGDAPPRRFYVL
                     PDKPAIAEDEQGNPIFSLIVYRRDEERIDPARSGEDVGGGILTFTVELKVPDETFEAI
                     RRELRMKVFGDASDPDRDVDLSYVPFLEGKVSVAVAGEAGDGGAGNEFVRTMVGAGKV
                     SGIGNNRKAVMVKLSQDGAALMSSIENLRTLPINVQYELSFEHRLQGVSMRVWLDMDS
                     SYVLIQETLHQDKEFDDGYLGLSENHVSIDKVTHVTEILTRNKTAGVTVIPQTSEIDD
                     ETLVQLEKFGFDMLNKEMEKALQASPPPAELDRTYLESFVAIYGQSFNFSLDRRMVLV
                     RNHTVSANVGNVFQGGNLAEMIAFVDLRIGFFTFLKVPIRVNADFSKLPVDSVTVTVI
                     YERQRFDGSGREERVDSFNFTDGSTIQTFLAYANRLDDVTYDWSATVHYKGEHRNFTF
                     NRKGVKDNFLVVDVGSLGMLQVDMGLGLVNIEKFPAARVSVRYRSQALGQMLEQSFLL
                     NKEKQSVSWTEVIHETMSPEGYEYKVDWLNAGGEVVQGEWQRSTASRQRFDAPVLGSF
                     EIAVTPSGNFKDGNDALSTIGVSLRYRDEENHYTEEGRLVFTDEKQQQTWTVEMLNPD
                     LRDYEYRYSLIYKDGVVKEVPEDGGWLKGQPGFLTVGEKYTLEVELFPTLLTYPDHAK
                     LVQVNLIYSDPANSVRSVDSFVFTKETSQPKTWRVRGVPGGPKTYTAEIAYFSAAGDV
                     ITVPPSVREAEVLVLPPPPAPVAPAPPPIT"
     gene            3721..4560
                     /locus_tag="VEL74_08705"
     CDS             3721..4560
                     /locus_tag="VEL74_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62648.1"
                     /translation="MATPAGEPDEKGFDLLLRCLAVPEPTVLPPLAEAFHRAADAGGA
                     PFEVIAGAPFRRYGARVTFRPTGEAGSLAAAFGLDSHPWGDPTWVGIRVRPDGSVQAK
                     AYHRLEGSLGRLDGRFVLPPGLPAGLRPVMAALHEGSRELYLRLGPAAPSWTSFAAAV
                     GAALEMNGIEKDLPFSPPPRPAEDGFCLSLRWQDGALTTLSLFADQRSLPGDGEIRRA
                     WPEGMDETDRNAYEAALAAVRGLGRLPPSHAWHGMLAWTLELSPEGSGFWHRAASLRV
                     PPL"
     gene            4580..>5795
                     /locus_tag="VEL74_08710"
     CDS             4580..>5795
                     /locus_tag="VEL74_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62649.1"
                     /translation="MLLLDSEIEVAGLTVFRDHASARTFHYLPGTPRVVTLDGGKPGL
                     QLLRYRGGREGGFLTLDVDLTLDETARTAAHDELRGRFGGAADLVPVLFHEGTVWLTA
                     LGVAAGSGDNRFVERVLGTTVPSLLGGARAIFSLELDREGASLVEAAIGTEGTPLAVV
                     YDLAYTGLRPARGLRARVRYGMAYDFLRTRLAANTLFFKADLDREAEALSREGHLEIE
                     DVDYQGTDPEILARRQEEIRATLRELSEALFFRPAASPDSVRPGAPSAIRPQAAFVLR
                     ALEQREEQELAYDLRETAAVTRRVAPQGMLRLPAGADPAELVLDVTFEGPAVTEVRAF
                     TLPGADWEGVEAIEVEVRGGGESRTLILSPETPDGAALLPPPSEEGPLEHRVRVLTRD
                     EPEALGTPPDPDT"
BASE COUNT          858 a         2012 c         2012 g          913 t
ORIGIN      
        1 gtcgcctggg tgaggagcac ccgtgagtcc gtcgtccccc gcacctcgta gcgggcgatt
       61 cccagcagac gctccagatc catgaagagg acgcggctgg tgccggaaag ggggggcagc
      121 atcgcccggc cgcctccttc gagagagccg aggggcgagg cggcgtcgaa cgtgacctcg
      181 gagcgcagcg tccaggagcc gtccggcgag gcgtcggacc aggattcgaa gcgcgcctcg
      241 gggctgccgg gggtcaaccg caagctctcg ctcgcggtac cgtactccac gtgcagaacc
      301 atctcggaga ggcccagctc ggccagcggc cgggctgtgc cgatgcgcag ggcaaagcgc
      361 cggaagaagg ggtgatccag gcccgcgtcg gcgatgtgct gatcaggaga cgctccgccc
      421 aggagatcgg cgagatgggc ctgcggggtg agcgtgcggg agaccacgat ggcgcgctgg
      481 aagtccacct cggccacggc gcgctcgatc tgcgtgaggc tcttcacggc ataggcggtg
      541 cccacgccga agatctcccc catcgtgccg agcgaggcgt gcgccggctc gtggtcgagc
      601 ggcatgaccg gctcgcagaa ccgctccacc agctcccgct gcacccaggc cagggccgct
      661 ccgggatcgg gctgcggcgc cccttcctcc ggcacgagcc cctggatcac ctgaatttcg
      721 atgacacggt tttcgatcag ccgctcggca agccgctgga cgtccgtcac cagcaggaat
      781 gagccttcct tgtgatggtc cgaaaagtga tcgtagaccc ggctccagtc gacccgcgct
      841 accacgcgct gggcgggcca gagaccctcc acggccagcc gatagatgat gccgatgggc
      901 gcgccaccgg agcgcagcgc cgcctcgacc agccgcgcgc cgtccggatc gagatcgacg
      961 gaaaacgccg cccggtaagg tggggcgaag ccggccgggg cgcggccgaa gccgcggcgg
     1021 atcagggtgg cgagcccccc ttcgccatcc ccgtcgaggc cggcgaagag caactccgcg
     1081 gtcgccacct cggccaggag cggccgcagc tcgatggccg gctcctcctc gcgaagctcg
     1141 gcggccagcg cttcgcgcac ctcgtcgagg cgggccggcg gatgggccag ctccacctcc
     1201 aaccgcagat ggccgccggc cagctcgccg tcgcgcacga agcggagcag ctccacctgc
     1261 ggcacccctt cggccaccgc cacccgcggg ggcggcggca gcacgtaata ggcgagcggc
     1321 ttgctgtccg cttgcccctc cgcgagcgcc aggtcgacgc cggcgacggt gcggaccgga
     1381 gcgttgagat cgatcaccgt cgtcttctcc taggtgatcg gaggcggcgc cggtgccaca
     1441 ggggccggcg gcggcggcag caccagcacc tcggcctcgc ggaccgacgg cgggacggtg
     1501 atcacatcgc ccgcggccga gaagtaggcg atctcggccg tgtaggtctt gggcccgccg
     1561 ggcacgccgc gcacgcgcca ggtcttcggc tggctggtct ccttggtgaa aacgaaactg
     1621 tccacggaac gcacggagtt cgccggatcg ctgtagatca ggttgacctg caccagcttg
     1681 gcatggtcgg gataggtgag gagcgtgggg aacagctcca cttcgagagt gtacttctct
     1741 cccacggtca ggaagccggg ctgtcccttc agccagccgc cgtcttcggg gacctccttc
     1801 accacgccgt ccttgtagat gaggctgtag cggtactcat agtcccgcag gtcggggttg
     1861 agcatctcca cggtccacgt ctgctgctgc ttctcgtcgg tgaagaccag ccgcccctcc
     1921 tcggtgtagt ggttctcctc atcgcgatag cgcagactca caccgatggt agagagggcg
     1981 tcgttgccgt ccttgaagtt gccggagggg gtcaccgcga tttcgaagct gccgaggacg
     2041 ggcgcgtcga agcgctgccg cgaggccgtc gagcgctgcc actccccttg caccacctcg
     2101 ccgccggcgt tcagccagtc gaccttgtac tcgtagcctt cgggagacat cgtctcgtgg
     2161 atcacctcgg tccacgagac gctctgcttc tccttgttga ggaggaagct ctgctcgagc
     2221 atctgaccca gcgcctggga gcgatagcgg acgctcaccc gcgccgccgg gaacttctcg
     2281 atgttcacga ggccgaggcc catgtcgacc tgcagcatgc cgagggagcc cacgtccacc
     2341 accaggaagt tgtccttcac ccccttgcgg ttgaaggtga agttcctgtg ctcccccttg
     2401 tagtggacgg tggccgacca gtcgtaggtc acgtcgtcga gacggttggc gtaggcgagg
     2461 aaggtctgga tggtggagcc atcggtgaag ttgaaggagt cgacccgctc ctcgcgcccg
     2521 ctgccgtcga agcgctggcg ctcatagatc acggtgacgg tgaccgaatc gaccggcagc
     2581 ttcgagaaat cggcgttgac ccggatggga accttcagga aggtgaagaa gccgatgcgc
     2641 aggtcgacga aggcgatcat ctcggccagg ttgccgccct ggaagacgtt tccgacgttc
     2701 gccgacacgg tgtgattgcg caccagcacc atgcggcggt cgaggctgaa gttgaagctc
     2761 tggccataga tggcgacgaa gctctcgaga taggtgcggt cgagctcggc cggcggcggc
     2821 gaggcctgga gcgccttctc catctccttg ttgagcatgt cgaagccgaa cttctcgagt
     2881 tgcaccagcg tctcgtcgtc gatctccgag gtctgcggga tgacggtcac gccggccgtc
     2941 ttgttgcggg tcaggatctc ggtgacgtgg gtgaccttgt cgatggagac gtggttctcg
     3001 gagaggccca ggtagccgtc atcgaactcc ttgtcctgat ggagggtctc ctggatcagc
     3061 acgtagctcg agtccatgtc gagccagacg cgcatggaga cgccctgcag ccggtgctcg
     3121 aaggagagct cgtactggac attgatgggg agcgtccgca gattctcgat gctggacatg
     3181 agagcggcgc cgtcctggct gagcttgacc atgaccgcct tgcggttgtt gccgataccg
     3241 ctcaccttgc cggcgcccac catggtgcgg acgaactcgt tgccggctcc tccatccccg
     3301 gcctcgccgg ccactgccac ggacaccttg ccctcgagga acgggacgta ggaaaggtcg
     3361 acgtcgcggt cgggatccga ggcgtcgccg aagaccttca tgcggagctc ccgccggatc
     3421 gcctcgaagg tctcgtccgg taccttcagc tcgacggtga aggtaaggat gccgccaccg
     3481 acgtcctccc cggagcgcgc gggatcgata cgctcctcat cgcgccggta gacgatcaat
     3541 gagaagatgg gattgccctg ctcgtcctcg gcgatggcgg gtttgtcggg cagcacgtag
     3601 aaacgccggg gaggggcgtc gcccgagggg gggcctccgg actgcagcgc ctggatctcg
     3661 tcggcgtcat cgtggtatac ggtgaaccct ttgatggtga gaggattggt catcgtcaac
     3721 atggcaactc ccgccggcga gccggatgaa aaaggtttcg atctgctact gcgctgtctc
     3781 gcggttcctg agccgactgt cctgcccccg ctggcggagg ctttccatcg ggcggcagac
     3841 gcggggggcg cgcccttcga ggtcatcgcc ggcgccccat tccggcgcta cggcgcccgt
     3901 gtcacgttcc gacccaccgg tgaggccggc tccctggcgg ccgccttcgg cctggacagc
     3961 catccgtggg gcgatcccac atgggtcggg atccgggtgc ggccggacgg ctcggtccag
     4021 gccaaggcct accaccgcct ggaggggagc ctggggcggc tggacggccg cttcgtcctg
     4081 ccgcccggcc tgcccgcggg gctccgcccg gtgatggccg ccctccacga ggggtcccgg
     4141 gagctctacc tgcggctcgg cccggccgcc ccctcgtgga cgtcgttcgc cgccgcggtg
     4201 ggcgcggcgc tggagatgaa cgggatcgag aaggacctcc ccttctctcc ccctcccagg
     4261 ccggccgaag acggcttctg tctcagcctg cgctggcagg acggcgcgct gaccaccctg
     4321 tcgctcttcg ccgatcaacg ctcgctgccc ggcgacggcg agatccgccg cgcctggccg
     4381 gagggcatgg acgagaccga ccggaacgcc tacgaggcgg ccctcgccgc ggtccgcggc
     4441 ctgggacgcc tgcctccgtc gcacgcctgg catggcatgc tggcctggac cctggagctg
     4501 tcccccgagg ggagcggctt ctggcaccgg gcggcgtcgc tgcgcgtgcc cccactctga
     4561 cctcattccg gaggaaccga tgctgctcct cgacagcgag atcgaagtcg ccggcctcac
     4621 cgtcttccgc gaccacgcct cagcgcgcac gttccactac ctccccggga cgccgcgcgt
     4681 cgtcaccctc gacggcggca agcccggcct ccagctcctc cggtatcgag ggggacgcga
     4741 gggcggcttc ctcaccctgg acgtggacct gacgctggac gagaccgcgc gcaccgccgc
     4801 ccacgacgag ctgcgcggcc gcttcggcgg cgcggccgac ctcgtgccgg tgctcttcca
     4861 cgagggcacc gtgtggttga ccgccctcgg ggtggcggct ggttccggcg acaatcgttt
     4921 cgtggagaga gtgctcggca ccaccgtgcc gtcgctcctc ggcggcgcgc gggccatctt
     4981 ctcgctggag ctcgaccgcg agggggcgtc gctggtcgag gcggcgatcg gcaccgaggg
     5041 gacgccgctg gccgtggtct acgacctggc ctacaccggc ctgcgccccg cccgcgggct
     5101 gcgtgcccgg gtccgctacg gcatggccta cgacttcctg cgcacccgtc tggcggcgaa
     5161 caccctcttc ttcaaggccg acctggaccg cgaggccgag gccctctccc gcgaggggca
     5221 cctcgagatc gaggatgtcg actaccaggg gaccgatccc gagatcctgg cgcggcgcca
     5281 ggaggagatc cgggcgacgc tgcgcgagct ctccgaggcg ctgttcttca ggccggccgc
     5341 ctcgcccgac tcggtgcgcc cgggtgctcc atcggcgatc cggccgcagg ccgcgttcgt
     5401 gctgcgcgcg ctggaacaac gggaggagca ggagctggcc tatgacctga gggagacggc
     5461 ggcggtcacc cgccgagtgg cccctcaggg gatgctgcgc ctgcccgccg gcgccgatcc
     5521 cgccgagctg gtgctggatg tcaccttcga agggcccgcc gtcaccgagg tgcgcgcctt
     5581 caccctgccc ggcgccgact gggagggggt cgaggccatc gaggtggagg tccgcggcgg
     5641 cggcgagtcg cgcaccctga tcctctcgcc ggagaccccg gacggcgcgg cgctgctccc
     5701 tcccccttcc gaggaggggc cgctggaaca ccgcgtccgc gtcctcaccc gggacgagcc
     5761 cgaggccctc ggcacgccgc cagaccccga cacgg
//
LOCUS       DASPJI010000330        13349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_651931, whole genome shotgun sequence.
ACCESSION   DASPJI010000330 DASPJI010000000
VERSION     DASPJI010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13349)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13349
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651931"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VEL74_08715"
     CDS             complement(<1..729)
                     /locus_tag="VEL74_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYG62650.1"
                     /translation="MRLGDLELTLVSDGEFRLDGGAMFGVVPRTLWEKQKPPDASNRI
                     RMGTNCLLVARGNDLLLIDTGIGDKNDAKFRAIYGMDPAAVRLPESIRQAGYEPGDVT
                     HVLLSHLHFDHCGWNTREEGGRVVPTFPNARYWLQRGEVEHARDPNDRDRASYDPRNW
                     EPLFEAGIVELFGDEAEPIPGVRAVRTPGHNADMCIVLIDGGGEKAVYWADLVPTAAH
                     VPYPWIMGYDLYPMTTLENKRRWLP"
     gene            complement(785..1300)
                     /locus_tag="VEL74_08720"
     CDS             complement(785..1300)
                     /locus_tag="VEL74_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYG62651.1"
                     /translation="MRTVLALLVSAALLVVAALAFIYSGLYDVAASAPDAGVVGWALG
                     TIQSRSVHRRAEEVAVPPLGDPAMIRQGLVLYHEMCAACHGAPGVAISAIGQGLNPPP
                     PELASEAEEPGELFWVTKHGIKMTGMPAFGVTLDDEELWAIVAFLQKMPEMSPEEYQA
                     AVRAAGPAPPL"
     gene            1329..2483
                     /locus_tag="VEL74_08725"
     CDS             1329..2483
                     /locus_tag="VEL74_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYG62652.1"
                     /translation="MSDPTSSHRGDLGLVLSGGGARAAYQAGVLRCLARNFPGLRFPI
                     VAGVSAGAINAAFLAGHTGPPAEAAPKLCELWGALEVENIFEVDVPSLARSFIQWATR
                     LASGGSALLAPDVKGLVDTRPLESTMRRATAVVDGELIGIEHNLERGLLKAIALTALN
                     YTTGQTVTWVQGADIGPSHHPQRPSFNTRMTVDHIMASAALPLFFPAVRLGDAWYGDG
                     GVRLTAPMSPALRLGASRILSISPHYSPNTEEAGRHQIRGYPPPAQILSHMLNSVFLD
                     VMDEDVRRLESINHLLAKIEPEERRGLRLVDILVIRPSRDLGKLAAAYEPRLPRAFRY
                     LTRSLGTRETSTPDFLTYIMFQPDYLQCLIEIGESDAEARLPELRELIGD"
     gene            2624..2929
                     /locus_tag="VEL74_08730"
     CDS             2624..2929
                     /locus_tag="VEL74_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62653.1"
                     /translation="MRSRSALLVVCLLVTAVAATPLSARDLRPPQPASASWLEEMTGW
                     VASLWEKLSGLAGGAEQGPKNLELPPPANGIEEGGETNPGGSCIDPNGPPSCGLSNS"
     gene            2938..4242
                     /locus_tag="VEL74_08735"
     CDS             2938..4242
                     /locus_tag="VEL74_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG62654.1"
                     /translation="MDAHSIPRDVLERFAANTATREEKTAVARHLFTGCPECTRRLEL
                     KLQRRAPENEDRQEEPYEKALDRCFGRVTSLLAAEPRELLEELESHPLRRQETMARNH
                     PRFREASLCDLLIERSRAARHRDPKSAFRSARLALLVASQLDGVPGEVLERRARAWAT
                     LGNAYRLQGDFLAAEKALQEAHKLAVAGDCDERLRAYVLSLWGSLRFDQRRFEESVRF
                     CRDCGAIHRRLGDAHEAGRALIQEGTALGESGRPGPGVRLLIEAARMIDIEREPTLAL
                     IAGHNIVRFYTDAGRTRDAMQLYLEMRDLYRQVNDPLIQLKAIWLEGQMLAAEAHPEP
                     ALTLLLRARSEFLRLGMTYEAALASLDLAAAFAKLGRFSALRDLVGRILPIFKALGVH
                     REAIASALLLRQSTDASAALQIIRSMAAQVKKTTWVLQRRAD"
     gene            complement(4267..4716)
                     /locus_tag="VEL74_08740"
     CDS             complement(4267..4716)
                     /locus_tag="VEL74_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62655.1"
                     /translation="MQGHPEERLLLERLRTLIDTRLGSVSVLEERLGLAPGALDELRP
                     DAAPPQSPLSLRWLYRILDILGIEPREFFSQVYELAPRAPLSTAGISGGIAEPEGSEF
                     PPFGRVLDTVRLLVRDAEDQQGPDGSEEEKNGEALNGEEAPEPPARR"
     gene            complement(4721..5809)
                     /locus_tag="VEL74_08745"
     CDS             complement(4721..5809)
                     /locus_tag="VEL74_08745"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HYG62656.1"
                     /translation="MATTMERVAGGSIDRITEHLGSEAESLLSYTCKGMPKEQLMLPG
                     PDHVERAWGSSDRKPGVLVNLQRLYGTGRLANTGYVSILPVDQGIEHSGAASFAPNPE
                     FFDPENIVRLAIEGGCNAVASTMGVLGALSRKYAHKIPFIAKINHNELLTYPNQFSQI
                     LFGQVEQAWDMGAAAVGATVYFGSEDSGREIVEIAEAFYRAHELGMATILWAYLRNNA
                     FKKDGKDYHTAADLTGQANHLAATMQADIVKQKQATLNGGYNAVSFGKTHPRVYSELV
                     ADHPIDWNRWQVVNCFMGRASLINSGGESKGQDDLGQAVRTAVINKRAGGAGLISGRK
                     AFQRPMREGVELLHAIQDVYLSPEVTIA"
     gene            5936..6601
                     /gene="trmH"
                     /locus_tag="VEL74_08750"
     CDS             5936..6601
                     /gene="trmH"
                     /locus_tag="VEL74_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019506391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HYG62657.1"
                     /translation="MSTPRRFHRVREVLDRRQPDLTVLLEDVQVPRNLAAILRSCDAA
                     GVFAAHAVWPGGRLKISRPASGGNRKWLPVHKHRTLDEALAHLRERGLRVLAAHPSPD
                     AAVPFREVDYTLPTALLLGNEDNGLTPAALERADGLVAIPMEGMGTSLNVSVAAALLL
                     FEAQRQRRAAGLYDAPRLDPETYARTLFEWAHPQLAAQCRKRQAPYPPLGPEGEILGP
                     LPR"
     gene            complement(6892..7806)
                     /locus_tag="VEL74_08755"
     CDS             complement(6892..7806)
                     /locus_tag="VEL74_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYG62658.1"
                     /translation="MRSLCALLPVAFLLAACASTATPTDPLAARVHQIAGRIAKRHGE
                     QAAMAVAYRDAGTGASLFVLPNQRMHPASTMKVPVMMALFDAVDRGEMRLDQPVPVRN
                     RFESIMDGSPYELDPAEDGDPDLYQAAGGTKTLEELIRRMIVRSSNLATNLLIERIGA
                     TRVTDLTRQLGAEDLRVLRGVEDDKAFQAGMNNVTTAKDLLVLLEALLPEAQGSPFSP
                     ASRERMLEILKAQEFNEKIPAGLPPGTPVAHKTGDITGIHHDAAVVFPPGERPYVLVV
                     LTRGILDEKEADATIAEISRAVWEERRR"
     gene            complement(7831..9294)
                     /locus_tag="VEL74_08760"
     CDS             complement(7831..9294)
                     /locus_tag="VEL74_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photolyase"
                     /protein_id="HYG62659.1"
                     /translation="MSNREPVCPEADYVLYWMIASRRLGWSFALDRAAGWARELGRPL
                     VVLEPLRCGYRWANDRIHRFVLDGMAEHARRLAGSNVLYHPYVEPSPGAGQGLLAALA
                     RRACVVVTDDYPAFFLPRMVAAAARQVSVRMEAVDSNGLLPLRAADRVFPAAYPFRRF
                     LQKELPRHLEDFPAEDPLEDLPPRLAALPEEVTRRWPAAPEALLRGERGGLAALPIDH
                     EVAPVDGVRGGDEAAAAALDRFLDERLLSYGEGRNDPDEEVTSGLSPYLHFGHVSSHQ
                     LLAALAARERWTPERLGRATTGAKEGWWGMSPAAEKFLDEAVTWREVGYNMAWQREDF
                     ERYESLPEWAQATLAKHEKDPRPHLYEHADFEAARIEDEIWNAAQTQLLREGRIHNYL
                     RMLWGKKVLHWSATPRQALASLIELNNRYALDGRDPNSYSGIFWCFGRYDRPWAPERP
                     VFGVVRYMSSDSTRRKLRLRGYLGRYAPGGQIPLLPG"
     gene            complement(9380..9730)
                     /locus_tag="VEL74_08765"
     CDS             complement(9380..9730)
                     /locus_tag="VEL74_08765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HYG62660.1"
                     /translation="MTSYSFGDILLVPFPFTDQSDSKKRPAVVVSSTAYQRERPDVIL
                     MAVTSQIRPSTGLGESLVTHWKDAGLLKPSLLKPILFTLERTLVLRRLGTLRGEDLIS
                     LRETLGVILGDGRG"
     gene            complement(9717..9923)
                     /locus_tag="VEL74_08770"
     CDS             complement(9717..9923)
                     /locus_tag="VEL74_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015170018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, antitoxin component, Xre
                     family protein"
                     /protein_id="HYG62661.1"
                     /translation="MANPLREQDLIEKIRMLPEEKLGEVEDFIDFLRLRTEDRRLTEA
                     AARLSEDALRRVWDNPDDADYDQL"
     gene            complement(9942..10535)
                     /locus_tag="VEL74_08775"
     CDS             complement(9942..10535)
                     /locus_tag="VEL74_08775"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HYG62662.1"
                     /translation="MTEPMRSWIMELRERLATPAPRRLGPKDLRQASVLVPLYVDAGE
                     LWTILTKRTDTLPSHRSQIAFPGGGRELKEDPWQAALREAQEEIGLDPKRVLKIGELD
                     EGETPAGFRVIPCVGAVPFPYEVNPNEAEISEVFSLPLSAFANPRLVEERTITLNGVD
                     RDIRIYHIGNRQIWGLTARILQNLLQRLGLETGEEME"
     gene            complement(10550..12301)
                     /locus_tag="VEL74_08780"
     CDS             complement(10550..12301)
                     /locus_tag="VEL74_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62663.1"
                     /translation="MGERSAVAETRTAAPRRLALGWWVLGLLLLATLAASIFFSPAAR
                     PPVGDEVTVAMAAASLAWDRDLTWAPADSERFAEQWGTRPDRLRLRPRDGGFTYDTPS
                     LYALLAAPMVRVAPVRGTIILNALLLAAAALLTARALSLHLGGAAPVWVTVLLFASVA
                     FAYVFRVQSDVFLLAATATGFALIYGGERSRRKDEPLPEMYEPEPNARGGAALVRWLA
                     AGALLAVPATVHPLYLLLLVPALLAATRVNRVVVVLGAAALLAAVLWTQGDLPDVQET
                     WGSEAPAFDMRSGTPREDRRSGSEEQREGMTLHPRLAGWNGLYFLAGRSTGVLPYFLP
                     LLLGFAAYRGDRGRWAILLAVVAIAAGFLLLRPFDFIGGEGAIANRLFLPLYAALWFL
                     AARPVRVLPALVVIILAVPFLYILWSHPAAVALAGEPRSYTSPVAERWLPYETTQRHA
                     PGRALFQNALWLKLLDRDLWTPRGADSLRLLGGAELLAGSSLPVQVFVLELDANAPSR
                     LEIGGQELRPVLLRPDGSLAFEVPAGEPRAVHPLWWSRGEDYYLYDLDFRLPGAPAGP
                     IRFRVLPRRDSMQRSDG"
     gene            complement(12280..>13349)
                     /locus_tag="VEL74_08785"
     CDS             complement(12280..>13349)
                     /locus_tag="VEL74_08785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG62664.1"
                     /translation="EEDPAIRVRAVPMGIPLPPAADAEAGRALRARFGLPADRPVLGS
                     FGFQTPIKRTEMAVRALARPGLEEVHLLIVGEAAGVMDLAGEARRVGVADRVHVTGFV
                     PFSDFEAAIAAVDLCLNLRYPTAGETSASLLRVLAAGRPAVVSDFAQFADLPSEVAVR
                     VPLGDGEPEALAALLADLLAQPGQLRRMGEAAREYVRSRHDLDRAAAAVAEACLEWRD
                     LDPPTEEEPRVQPPSSIGWESLPGDLEVAGADLPWPEGERRSLRIRLRNRGFARWLAG
                     ERGPGGVAVVVKLFSGDQDLYEGQRWLALPRDLAPGEEVEFETEIRRPPGSSRLWIEP
                     HMFGGLGFSKLGGPLWVRDLQ"
BASE COUNT         2011 a         4775 c         4601 g         1962 t
ORIGIN      
        1 cggcagccag cgcctcttgt tctccagagt cgtcatcgga tacaggtcgt accccatgat
       61 ccaggggtag gggacgtgag cggcggtggg caccaggtcg gcccagtaga cggccttctc
      121 gccccctcca tcgatgagaa caatacacat gtcggcgttg tgcccggggg tgcggaccgc
      181 ccggacgccg gggatcggct cggcctcgtc gccgaacagc tccacgatgc cggcctcgaa
      241 cagcggctcc cagttgcgcg ggtcgtagct cgcccggtcc cggtcattgg gatcgcgggc
      301 gtgctccacc tcgccgcgct gcagccagta cctggcgttg gggaaggtcg gcaccacccg
      361 gcccccctcc tcccgcgtgt tccagccgca gtggtcgaag tggaggtggg agaggagcac
      421 gtgagtgaca tcccccggct cgtagccggc ctgccggatc gactcgggca ggcgcaccgc
      481 cgccgggtcc atgccgtaga tcgcgcggaa cttggcgtcg ttcttgtcgc cgatgccggt
      541 atcgatgagc agcaggtcgt tgccacgggc gaccagcagg cagttggtcc ccatgcggat
      601 gcggttcgac gcgtccggcg gcttctgctt ctcccaaagc gtccgcggga cgacgccgaa
      661 catggcgccg ccgtccaggc gaaattctcc gtcgctgacc agcgtcagct cgaggtctcc
      721 cagccgcatc ggcgggaagt ctatccgaag gaatccgcga ggggccaccc ggccagctac
      781 ctggtcagag cgggggcgcc ggtccggcgg cgcggacggc ggcctggtac tcctcggggg
      841 acatctcggg catcttctgg aggaaggcga cgatggccca gagctcctcg tcgtccaggg
      901 tcaccccgaa agcgggcatg ccggtcatct tgatgccgtg cttggtgacc cagaacagct
      961 cccccggctc ctccgcctcg ctcgccagct cgggaggcgg ggggttgagc ccctggccga
     1021 tggcggagat ggcgacgccc ggggcgccgt ggcaggcggc gcacatctca tggtagagga
     1081 cgaggccttg ccggatcatg gccgggtcgc cgagcggcgg gacggccacc tcctcggccc
     1141 gccggtgcac cgagcgcgac tggatggtgc ccagggccca gccgaccacg ccggcgtcgg
     1201 gagccgaggc ggcgacgtcg tagaggcccg agtagatgaa ggccagcgcg gccaccacca
     1261 gcagagccgc ggacaccagc agcgccaaga ccgttctcat ttctcccttc ctaccgtaaa
     1321 gtggatccgt gtccgacccg acatcatctc atagaggcga cctgggcctg gtgctctcgg
     1381 gcggcggggc gcgggcggcc taccaggccg gcgtgctccg gtgcctcgcc cgcaacttcc
     1441 cggggctgcg cttccccatc gtcgccgggg tctcggccgg cgccatcaac gccgccttcc
     1501 tcgccggcca caccggcccg ccggccgaag cggcgccgaa gctctgcgag ctctggggcg
     1561 cgctggaggt cgagaacatc ttcgaggtgg acgtgccgtc cctcgcccgc agcttcatcc
     1621 agtgggcgac gcgcctcgcc tcgggcggct cggccctcct cgcgcccgac gtgaaggggc
     1681 tggtggacac ccgcccgctg gagagcacca tgcgccgggc gaccgccgtg gtggacggcg
     1741 agctcatcgg catcgagcac aacctggagc gcgggctcct caaggccatc gccctgaccg
     1801 ccctgaacta caccaccggg cagacggtga cctgggtgca gggggcggac atcggcccct
     1861 cgcaccaccc ccagcgcccc agcttcaaca cccgcatgac cgtggaccac atcatggcct
     1921 cggccgccct gccgctcttc tttcccgcgg tacgcctggg ggacgcctgg tacggcgacg
     1981 gcggcgtgcg cctcaccgcc ccgatgagcc cggcgctgcg cctgggcgcg agccgcatcc
     2041 tctccatctc gccccactat tcacccaata ccgaagaggc cgggcgccac cagatccggg
     2101 ggtacccgcc gccggcgcag atcctctccc acatgctcaa ctcggtcttc ctggacgtga
     2161 tggacgagga cgtgcgccgg ctcgagagca tcaatcacct cctcgccaag atcgagccgg
     2221 aggagcgccg aggcctgcgg ctggtcgata tcctggtgat ccgcccctcc cgcgacctgg
     2281 gcaagctcgc cgccgcctac gagccgcgcc tcccccgcgc cttccgctat ctcacccgca
     2341 gcctcggcac ccgcgagacc agcacgccgg acttcctgac ctacatcatg ttccagccgg
     2401 actacctgca gtgcctcatc gaaatcggcg agtccgacgc cgaggcccgc ctgcccgagc
     2461 tccgggagct gatcggggat tgacaactca tagataatcc gtgataaggt gaatcgtcac
     2521 cctcttgtca cgagttggcc ttttgggcta cttacttgtt taggagctgt cctcggatgc
     2581 gggttgcatc cgtcgttccc tgtgttctgt tgagaggaga gaaatgagat cccggtccgc
     2641 actgctcgtt gtctgcctgc tcgttacggc cgttgccgcc acgccgctgt ccgcgcgtga
     2701 cctgcggccc cctcagcccg cctccgccag ctggctggag gagatgaccg gctgggtcgc
     2761 ctctctatgg gaaaagctct ccggcttggc cggaggagcc gagcagggcc cgaagaacct
     2821 ggagctgccg ccgccggcca acggaatcga ggagggtggt gagacgaacc ccgggggctc
     2881 ctgcatcgat cccaacggtc cgcccagttg cggcctttcc aactcgtagt tgtcgccatg
     2941 gacgctcatt caattccccg ggacgtcctc gagcggttcg cggccaacac cgcgacccgg
     3001 gaggagaaaa cggcggtggc gcgccacctc ttcaccggct gcccggagtg cacccgccgg
     3061 ctcgagctca agctgcagcg ccgcgcgccc gagaacgagg accggcagga ggagccctat
     3121 gagaaggctc tggaccgttg cttcggacgg gtcacctcgc tgctggccgc ggagccgcgg
     3181 gagctcctgg aagagctcga gtcccatccc ctgcgccggc aggagacgat ggcccgcaac
     3241 cacccgcgct tccgcgaggc gagcctgtgc gacctgctga tcgagcgcag ccgcgcggcg
     3301 cggcaccggg atccgaagag cgccttccgg tcggcccgcc tggccctgct ggtggccagc
     3361 cagctcgatg gcgtccccgg cgaggtgctg gagcgccgcg cgcgcgcctg ggcgaccctg
     3421 ggcaacgcct accgtctcca gggtgatttc ctggcggccg agaaggcgct gcaggaggcg
     3481 cacaagctcg ccgtggccgg ggactgcgac gagcgtctca gggcctatgt gctcagcctc
     3541 tggggatcgc tgcggttcga ccagcggcgc ttcgaggagt cggtccgctt ctgccgcgac
     3601 tgcggcgcca tccaccgccg gctcggcgac gcgcacgagg cgggacgcgc cctgatccag
     3661 gaggggaccg ccctcggcga gtcgggccgc ccgggtccgg gcgtgcgcct gctcatcgag
     3721 gcggcgcgga tgatcgacat cgagcgcgag ccgacgctgg cgctgatcgc cggtcacaac
     3781 atcgtccgct tctacacgga cgcgggccgg acccgtgacg ccatgcagct ctacctcgag
     3841 atgcgggacc tctaccggca ggtgaacgat ccgctgatcc agctcaaggc gatctggctc
     3901 gaggggcaga tgctggcggc cgaggcccac cccgaacccg ccctgaccct gctgttgcgc
     3961 gcccgttcgg agttcctgcg tctggggatg acctacgagg cggccctggc gtcgctggac
     4021 ctggcggcgg ccttcgccaa gctggggcgc ttctcggcgc tgcgcgacct cgtcggccgg
     4081 atcctcccca tcttcaaggc cctgggagtc caccgcgagg ccatcgcctc ggctctgctc
     4141 ctgcgccagt cgaccgacgc ctccgccgcc ttgcagatca tccgcagcat ggcggcccag
     4201 gtgaagaaga cgacctgggt cctccagcgc cgcgccgact gaccccagag gctccgaagc
     4261 ccccgatcac cgccgtgccg gcggctcggg ggcttcttcc ccgttcagag cctccccgtt
     4321 cttctcttct tcggagccgt ccggtccctg ctggtcctcc gcatcccgca cgagcagccg
     4381 caccgtgtcc agcacccggc cgaaaggagg gaactcgctc ccctccggct ccgcgatgcc
     4441 ccctgagatg ccggccgtgc tcaacggggc acgcggcgcc agctcgtaga cctgggagaa
     4501 gaactctctc ggctcgatcc ccaggatgtc caggatgcgg tagagccacc gcaggctgag
     4561 cggggactgc ggtggcgccg cgtccggacg cagctcgtcg agcgcgccgg gcgcgaggcc
     4621 caggcgctcc tcgaggaccg acaccgatcc cagacgcgta tcgatcagcg tccgcagcct
     4681 ctcgaggagg agcctctctt ccggatggcc ctgcatgagg tcaggcgatc gtgacctcgg
     4741 ggctgaggta gacgtcctgg atcgcgtgga gcagctcgac cccctcgcgc atcggccgct
     4801 ggaacgcctt gcggccggag atcaggccgg cgccgccggc gcgcttgttg atcaccgccg
     4861 tgcgcaccgc ctggccgagg tcgtcctgcc ccttggactc gccgccggag ttgatgaggc
     4921 tcgcccggcc catgaagcag ttgaccacct gccagcggtt ccagtcgatc gggtggtcgg
     4981 ccaccagctc gctgtagacc cgggggtggg tcttgccgaa gctgaccgcg ttgtagccgc
     5041 cgttcagcgt cgcctgcttc tgcttgacga tgtcggcctg catggtggcg gccaggtggt
     5101 tcgcctggcc ggtgaggtcg gccgcggtgt ggtaatcctt gccgtccttc ttgaaggcgt
     5161 tgttccgcag gtaggcccag aggatcgtcg ccatccccag ctcatgagcg cgatagaacg
     5221 cctcggcgat ctcgacaatc tcccggcccg agtcctcgct gccgaagtag accgtggcgc
     5281 cgacggccgc cgcccccatg tcccaggcct gctcgacctg gccgaacagg atctggctga
     5341 actggttggg gtaggtcagc agctcgttgt ggttgatctt ggcgatgaac gggatcttgt
     5401 gggcgtactt gcgggagagc gcgccgagaa cccccatggt ggaggccacg gcgttgcagc
     5461 cgccctcgat cgccagccgg acgatgttct ccgggtcgaa gaactcgggg ttcggggcga
     5521 aggaggccgc gccggagtgc tcgatcccct ggtccaccgg caggatcgag acatagccgg
     5581 tgttggcgag gcggccggtg ccgtagaggc gctggaggtt gaccagcacg ccgggcttgc
     5641 ggtctgagga gccccaggcc cgctccacgt ggtccgggcc gggcagcatg agctgctcct
     5701 tgggcatccc cttgcaggta taggaaagga gactctcggc ctcgctgccg aggtgctcgg
     5761 taatgcggtc gatgctgccg cccgcaacgc gttccatggt cgtggccatc cgcaactcct
     5821 ccgtgaacag acttcttcgg gacagggttt tcggggtgcg ccgctacggc ggcgcccgcc
     5881 tatcctatca ggcgaacgcg acgccgccgc gtgctcgtga tagatagcgc atgcaatgag
     5941 cacgccgcgc cgtttccatc gcgtccggga ggttctggac cgccgccagc ccgacctcac
     6001 ggtcctgctg gaagacgtcc aggtgccgcg caacctggcc gccatcctgc ggagctgcga
     6061 cgccgccggc gtcttcgccg cccacgcggt ctggccgggc gggcgcctga agatctcgcg
     6121 gcccgcctcg ggcggcaacc gcaagtggct gccggtgcac aagcacagga cgctggacga
     6181 ggccctcgcc cacctgcggg agcgcggctt gcgggtgctg gcggcccacc cctcgccgga
     6241 cgccgccgtc cccttccgcg aggtggacta cacgctcccc accgcgctcc ttctgggaaa
     6301 cgaggacaac ggcctgacgc cggcggcgct ggagagggcc gatggcctgg tggcgatccc
     6361 catggagggg atgggcacgt cgctcaacgt ctcggtggcc gcggcgctcc tcctcttcga
     6421 ggcccagcgc cagcggcggg ccgccggcct ctacgacgcg ccgcgcctcg atcccgagac
     6481 ctacgcccgc accctcttcg aatgggccca tccgcagctc gccgcccagt gccgcaagcg
     6541 ccaggcgccc tacccgccgc tcggccccga gggcgagatc ctgggtccgc tgccccggta
     6601 gccgcggaac ccatcccact cgacgggaca ccatccggag cgctcgggaa gcttcccggg
     6661 ccgtctggaa tccatcctat tgaattggac ggcatccgga gcgcccggac tccatccgga
     6721 tgagctggac gcgttccgag gcgcccggaa gcttcccgcg ccgtctggat ggcttccaag
     6781 cgaaccggat gagatccgga gcgtccggga aacatcctga gctcccggat tccatccgcg
     6841 ccgcccggac gggatctcgg gcgcccgggc ggcacccccg gcgcccggcg gttaccggcg
     6901 ccgctcctcc cagaccgccc gcgagatctc ggcgatcgtg gcgtcggcct ccttctcgtc
     6961 gaggatgccg cgggtgagga cgaccaggac gtagggcctc tcccccgggg ggaagacgac
     7021 cgccgcgtcg tggtggatgc cggtgatgtc acccgtcttg tgggccaccg gcgtcccggg
     7081 aggaaggccg gcggggatct tctcgttgaa ctcctgagcc ttgaggatct ccagcatccg
     7141 ctcccgcgag gccggggaga acggcgagcc ctgggcctcg ggcaggagcg cctccaggag
     7201 gaccagcagg tccttggcgg tggtgacgtt gttcatcccc gcctggaagg ccttgtcgtc
     7261 ctcgacgccg cgcagcaccc ggaggtcttc ggcgccgagc tgccgcgtca ggtccgtcac
     7321 ccgggtggcg ccgatcctct cgatgaggag gttggtggcg aggttggagc tgcggacgat
     7381 catccgccgg atcagctcct ccagggtctt cgtgccgccc gccgcctggt acaggtcggg
     7441 gtcgccgtcc tccgccgggt ccagctcgta gggggagccg tccatgatgc tctcgaagcg
     7501 gttgcggacg gggaccggct ggtccaggcg catctcgccg cggtccaccg cgtcgaacag
     7561 cgccatcatc accggcacct tcatcgtgct cgccggatgc atccgctggt tggggaggac
     7621 gaagaggctg gccccggtcc ccgcgtcgcg gtaggccacg gccatggcgg cctgctcgcc
     7681 gtgccgcttc gcgatccggc cggcgatctg gtggacccgg gccgccagcg ggtcggtcgg
     7741 ggtcgcggtc gaggcgcagg cggcgaggag gaacgccacg gggaggagcg cgcagaggga
     7801 tctcatggcc gtggattcta ctcgggctgc tcatcccggc agcagcggga tctggccgcc
     7861 gggggcgtag cggccgaggt agccgcgcag gcgcagcttg cgccgggtgc tgtcggagct
     7921 catgtagcgg accacgccga aaaccgggcg ctcgggggcc caggggcgat catagcggcc
     7981 gaagcaccag aagatgccgc tgtaggagtt gggatcgcgg ccgtccaggg cgtaccggtt
     8041 gttcagctcg atcaggctcg ccagcgcctg ccgcggcgtc gccgaccagt ggagcacctt
     8101 cttgccccag agcatccgca ggtagttgtg gatgcgcccc tcgcgcagga gctgcgtctg
     8161 ggcggcgttc cagatctcgt cctcgatacg ggcggcctcg aaatcggcgt gctcatagag
     8221 atgggggcgc gggtccttct cgtgcttggc gagggtcgcc tgcgcccact cgggcagcga
     8281 ctcgtagcgc tcgaagtcct cccgctgcca ggccatgttg tagccgacct cgcgccaggt
     8341 gacagcctcg tccaggaact tctcggccgc cgggctcatc ccccaccacc cctccttggc
     8401 acccgtggtg gcgcgcccca gccgctccgg cgtccagcgc tcccgggcgg ccagggcggc
     8461 gaggagctga tgcgaggaga cgtggccgaa gtggaggtag ggggagaggc cgctggtgac
     8521 ctcctcatcg ggatcgttcc gtccctcgcc atagctcagc agccgctcgt cgaggaagcg
     8581 gtcgagagcc gcggcggcgg cttcgtcccc gccgcggaca ccgtccaccg gcgccacctc
     8641 gtgatcaatg gggagcgccg ccagcccccc gcgttcgccg cgcagcaacg cctccggcgc
     8701 cgcgggccag cggcgggtca cctcctccgg gagcgcggcg aggcgcggcg gcaggtcctc
     8761 gagcgggtcc tccgcgggga agtcctccaa gtgcctgggc agctccttct gcaggaaacg
     8821 gcggaaaggg taggccgcgg gaaagacccg gtcggcggcg cgcaggggca ggagcccgtt
     8881 cgaatccacg gcctccatcc gcaccgatac ctggcgggcg gcggccgcca ccatccgggg
     8941 aaggaagaaa gccgggtagt cgtccgtcac caccacgcag gcgcgccggg cgagggcggc
     9001 gaggagccct tggccggcac cgggggacgg ctccacatac gggtgataga ggacgttcga
     9061 gccggccagg cgccgggcgt gctccgccat gccgtcgagg acgaaacggt ggatgcggtc
     9121 attcgcccac cggtagccgc agcgtagcgg ctcgaggacc acgagcgggc gccccagctc
     9181 ccgggcccag cccgccgccc ggtccagggc gaagctccag cccagacgcc tcgaagcgat
     9241 catccagtac aggacgtaat cagcttcggg acaaaccggc tcccgattgg acatccgaac
     9301 gcggatctcg ggtacgttgg tcacaagatc tctacccgtg gctggattga agtagcaccc
     9361 ccggacaggg agcagcaggt cagccccgcc catcccccag gatgaccccc agggtttccc
     9421 gaagagagat cagatcttcg cctctcaggg ttccgagccg ccgtagtacg agagtcctct
     9481 ccagagtaaa gaggatcggc ttcagaaggg aaggcttgag gagcccagcg tccttccaat
     9541 gagtgacgag cgattcgccc aatcccgtgg atggcctgat ctggctggtc acggccatca
     9601 ggatcacgtc cggccgctct ctctgatagg cggtggagct gaccacgacg gctggacgct
     9661 tcttgctgtc ggactgatcg gtaaaaggaa aggggacgag aaggatgtcg ccaaagctat
     9721 agctggtcat agtcggcgtc atcaggattg tcccagactc tgcgcaatgc atcctcggac
     9781 agccgcgccg cagcttcggt gaggcgccga tcttcggtcc gaagtcgaag gaaatcaatg
     9841 aagtcctcca cttcgccgag cttctcctcc ggcaacatcc gtatcttctc gatcaggtcc
     9901 tgctctctca gaggatttgc catgccttat tgtacgccgg cctactccat ctcctccccc
     9961 gtctccagcc ccagccgctg cagcaggttc tggaggatgc gggcggtcag gccccagatc
    10021 tggcggttgc cgatgtggta gatgcggatg tcccggtcga cgccgttgag ggtgatggtg
    10081 cgctcctcca ccaggcgagg gttggcgaag gcgctcaagg ggaggctgaa gacctcggag
    10141 atctcggctt cgttgggatt cacctcgtaa gggaagggca cggcgccgac gcaggggatc
    10201 acccgaaatc ccgccggggt ctccccctcg tccagctcgc cgatcttcag gacccgcttg
    10261 ggatcgagcc cgatctcctc ctgggcctcc cgcaaggccg cctgccaggg gtcctccttg
    10321 agctcgcggc cgccgcccgg gaaagcgatc tggctgcggt ggctggggag ggtgtccgtg
    10381 cgcttggtga ggatcgtcca cagctcgccg gcgtcgacgt agaggggcac cagcaccgag
    10441 gcctggcgga ggtctttcgg acccagccgc cgcggcgccg gagtcgcgag ccgctcccgc
    10501 aattccatga tccagcttct catcggctcg gtcatacgct caacatcctt cagccgtcgc
    10561 tcctttgcat cgaatcccgg cgcggcagga cgcggaaccg gatcggaccc gccggcgccc
    10621 cgggcagcct gaagtcgaga tcatagaggt aatagtcttc tccccgcgac caccagagcg
    10681 gatgcacggc gcgcggctcc cccgccggca cctcgaaggc gagcgagccg tccggacgca
    10741 gcaggaccgg acgcagctcc tgtcctccga tctccaggcg cgagggcgcg ttggcgtcga
    10801 gctccaggac gaacacctgg accggcagcg agctcccggc cagcagctcc gcaccgccga
    10861 ggagacgcag gctgtccgcc ccgcgtggag tccagaggtc ccggtcgagg agcttgagcc
    10921 agagggcgtt ctggaagagt gcccggccgg gagcgtggcg ctgggtggtc tcatagggca
    10981 gccaccgctc ggccaccggc gacgtgtagg agcgtggctc tccggcgagc gcgaccgccg
    11041 cggggtggct ccacaggatg taaaggaagg gaacggccag gatgatgacc accagcgccg
    11101 gcagaacccg caccggccgg gccgccagga accagagggc ggcgtagagc ggcaggaaca
    11161 gccggttggc gatggccccc tcgccgccga tgaagtcgaa gggacgcagg aggaggaagc
    11221 cggcagcgat cgccaccacc gccagaagga tggcccagcg cccccgatcc cctcgatacg
    11281 cggcaaaccc caggagcagc ggcaggaagt acgggagcac accggtgctc ctccccgcca
    11341 ggaagtagag gccattccag cccgcgagcc gggggtggag cgtcatcccc tccctctgct
    11401 cctcgctgcc gctccgccgg tcctcgcgcg gcgtgccgct ccgcatgtcg aaggccggcg
    11461 cctcgctccc ccaggtctcc tggacatctg gcaggtcccc ctgcgtccag agcaccgcgg
    11521 ccaggagcgc cgccgcgccg aggaccacca ccacccggtt gacccgggtc gcggccagca
    11581 gcgccggaac caggagcagc agatagagag gatggacggt cgcgggaacg gcgagcagcg
    11641 ctccggcggc cagccagcgg acgagcgccg cccctccccg tgcgttgggc tccggctcgt
    11701 acatttccgg cagcggctcg tctttccggc gggagcgctc gcctccgtag atcagagcga
    11761 agccggtggc ggtcgcggcc agcaggaaga cgtcggactg gaccctgaag acgtatgcga
    11821 aggcgaccga ggcgaagagc aggaccgtta cccagaccgg cgccgcccct cccaggtgca
    11881 gggagagcgc gcgggcggtc agcagagccg ccgccgccaa cagcagcgcg ttgaggatga
    11941 tcgtcccgcg gaccggagcg acgcgcacca tgggggccgc cagcagcgcg tacaggctcg
    12001 gcgtgtcgta ggtgaatccg ccatcccgcg gccggaggcg caggcggtcc gggcgggtcc
    12061 cccactgctc ggcaaagcgc tcagaatccg ccggtgccca ggtgaggtcc cggtcccagg
    12121 cgaggctcgc cgcggccatg gccacggtga cctcgtcgcc gacgggcggc cgcgccgcgg
    12181 gactgaagaa gatagaggca gccagggtcg ccaggagcag gagccccagg acccaccatc
    12241 cgagggcaag ccgccgcggc gcggccgttc gggtctcggc tactgcagat ctctcaccca
    12301 cagggggcct cccagtttcg agaaccccag gccgccgaac atgtggggct cgatccacag
    12361 gcgcgaggag ccggggggcc gccggatctc ggtctcgaat tccacctcct cacccggcgc
    12421 cagatcgcgc ggcagggcga gccagcgctg accttcatag aggtcctggt ctcccgagaa
    12481 gagcttgacc acgaccgcga ctccgcccgg ccctctttcc ccagccagcc agcgggcgaa
    12541 gccccggttc cggagccgga tgcggagcga ccgccgctcc ccttcgggcc aggggagatc
    12601 cgcgcccgcc acctccaaat cccccggcag gctctcccag ccgatgctgc tcggcggctg
    12661 cacccgcggc tcctcctctg tgggcggatc cagatcccgc cactccaggc aggcctcggc
    12721 cacggctgcg gcggcccggt ccaggtcgtg acgggaacgg acgtactcgc gggcggcctc
    12781 gcccatccgc cgcagctggc cgggttgagc gagaagatcc gccagcagcg cggccagcgc
    12841 ctccggctcc ccgtcgccga gcggcacgcg gaccgccacc tcggagggga ggtcggcgaa
    12901 ctgcgcgaag tcggagacca cggccggccg gcccgccgcc agcacccgca gcagcgaggc
    12961 cgacgtctcc cccgccgtgg gatagcggag gttgaggcag aggtccacgg cggcgatggc
    13021 ggcctcgaag tccgagaacg gcacgaagcc ggtgacgtgc acccggtccg ccactccgac
    13081 ccgccgcgcc tcccctgcga ggtccatgac gccggcggcc tcgccgacga tcagcagatg
    13141 gacctcctcg aggccgggcc gggccagcgc ccgcaccgcc atctcggtcc gcttgatggg
    13201 cgtctggaag ccgaaggagc cgaggaccgg ccggtccgcc ggcagcccga agcgggcgcg
    13261 cagggcccgt cccgcctccg cgtccgccgc cggcggcagg gggatgccca tcggcaccgc
    13321 ccgcacccgg atcgccgggt cctcctcgc
//
LOCUS       DASPJI010000331         3165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_826946, whole genome shotgun sequence.
ACCESSION   DASPJI010000331 DASPJI010000000
VERSION     DASPJI010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826946"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="VEL74_08790"
     CDS             <1..1282
                     /locus_tag="VEL74_08790"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HYG62665.1"
                     /translation="YAAGDEAPLALKQAPVAPPEPEAAVQTEDLSEARQEEPRSTAPN
                     PTAGGAPDSPQPLAAQQEQPQHETETESLPVHREAGAAPPARQRLDRKAGELPGSSAP
                     LPAAPAPAPPPPPAAAEAAAPQGFVAFEEITVTGVASEARSSSTGSSAGLRRSADGFV
                     FTERAPLSPFGLDVGTASYETARHSLGEGELPGPGSVRVEEWVNYFHYGDRAPLRTDF
                     NVTAEGAPSPFTPGAQYRLVRFNIRGREVDPRNRVIAREAWAQVEWNATMVERYRLLG
                     FENGGSAGPRRGLQSAGDEIEAGHSVTALYEIKLQPGIEEEADRRLASLRLRWRSPET
                     GRVSETETPLMGFHIATGWEAASPALRLAAVVAEMAEILAGSPAAREGSLDTVFQEAQ
                     RIGGRFPGDAEVAEFVSLAGKAARLKGRAAEEPR"
     gene            complement(1251..>3165)
                     /locus_tag="VEL74_08795"
     CDS             complement(1251..>3165)
                     /locus_tag="VEL74_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HYG62666.1"
                     /translation="YVPPRWADRARRSFGKTPEMMEYFSTVTGRPYPYAKYAQTAVAD
                     FTWGGMENITATTQTLRTLHDERAHNDSRSDSLVAHELAHQWFGDLVTCEDWSHLWLN
                     EGMADYLTVLYLGHAGGAEEAAVELETIRQAYLREDARRYRRPIVTDRYPDPITLFDR
                     HSYEKGALVMRMIHGLLGEEGWWKGIRAYLDRFALQTVKTSDFQSVLEKSTGVTLGPL
                     FDQYVYGAGHPELEVRWDYQADRELVRLEVRQTQEVTGETGLFSFPVEIGLAGDVGDG
                     GLEIQTVQILARAEQEIYLHSPARPRTVVFDPRDWVLAAVDFDKPTAEWVAQLSAAAG
                     AAETPARLEALRALGELADAPAVEALAKALRQDPFHGARQVAAEALGQAGTEAARQAL
                     EAGAGDRDSRVRTMVLASLGNFPRDRESLPLLRRALEGDDSYKARAAAATALGRRRDA
                     AREVTPWLVRALSQESHQEDVRAAAIEALAELDSPAVVDHAFRLAAYGAPAESRDEAV
                     TALARIAARRQDPELRARVRRTLEGILAERDSRMREEALRALMELGDAEAIPALERTG
                     RTDPDPELRLRAGQVIERIRGNAAAGPSGDGEGLRLRVEQLEREIDVLKARLEEAQGT
                     LEGATGAPPPPSP"
BASE COUNT          400 a         1169 c         1164 g          432 t
ORIGIN      
        1 gtacgctgcc ggggatgagg cgccgctggc gttgaagcag gctccggtgg cgcctcccga
       61 gcccgaggcc gccgtccaga ccgaagacct gtccgaggct cggcaggagg agccgcggag
      121 cacggcgccg aatcccaccg ctggcggggc tcccgattct ccgcagcccc tggcggccca
      181 gcaggagcag ccgcagcatg agacggagac ggaatccctg cccgtccaca gggaagccgg
      241 cgcggcccct cctgccagac agcgcctgga ccggaaggcc ggcgagctgc ccggttcctc
      301 cgcgccgctg ccggcggccc cggcgccagc cccgccgcct ccgccagcgg cggcggaggc
      361 ggcggctccc caggggttcg tggccttcga ggagatcacc gtcaccgggg tcgccagcga
      421 ggcgcggtcc tcgtccacgg gctccagcgc cggcctccgc cggtccgccg acggcttcgt
      481 cttcaccgag agggcaccgc tgtcgccctt cgggctggac gtgggcaccg cctcctacga
      541 gacggcccgc cattctctcg gcgagggcga gctgccgggc cccggctcgg tgcgggtgga
      601 ggagtgggtg aactacttcc actacggtga ccgagcgccg ttgcgcaccg acttcaacgt
      661 caccgccgag ggcgctccct ccccgttcac cccgggagcg cagtaccggt tggtgcgctt
      721 caacatccgc ggccgggagg tggacccccg gaaccgcgtg atcgccaggg aggcctgggc
      781 gcaggtggag tggaacgcga ccatggtgga gcgctaccgg cttctcggct tcgagaacgg
      841 cggcagcgcc ggcccgaggc gtggcctgca gagcgcgggc gacgagatcg aggccggcca
      901 ctcggtgacc gcgctctacg agatcaagct gcagcccggc atcgaggagg aggcggaccg
      961 ccgtctcgcc agcctgcgcc tgcgctggcg ctcgcccgag acgggacggg tctccgagac
     1021 cgagacgccc ctgatgggct tccatatcgc caccggctgg gaggccgcgt cgccggccct
     1081 gcgcctcgcg gccgtggtcg ccgagatggc cgagatcctc gccggctctc ccgcggccag
     1141 ggagggcagc ctcgacacgg tcttccagga ggctcagcgg atcggcggcc ggttccccgg
     1201 tgacgccgag gtggccgaat tcgtcagcct ggcgggcaag gcggcgcggc tcaaggggag
     1261 ggcggcggag gagccccggt agccccctcc aacgtcccct gcgcctcttc cagccgcgcc
     1321 ttgagcacgt cgatctcgcg ttcgagctgc tcgacgcgca ggcgcaggcc ctcgccgtct
     1381 cccgatgggc cggcggcggc gttgccgcgg atcctctcga tgacctggcc ggcgcgcaag
     1441 cggagctcgg gatcggggtc ggtccggccg gtgcgctcca gggcggggat ggcctccgcg
     1501 tctcccagct ccatcagggc ccggagggcc tcttcgcgca tccggctgtc gcgctcggcg
     1561 aggatcccct ccagggtgcg gcggacccgg gcccgcagct ccgggtcctg gcggcgcgcg
     1621 gcgatccggg cgagggcggt gaccgcctcg tcccggctct cggcgggggc gccgtaggcg
     1681 gccaggcgga acgcgtggtc cacgacggcg ggggagtcca gctccgccag cgcctcgatc
     1741 gccgcggccc gcacgtcctc ctggtggctc tcctgggaga gggcccggac cagccagggg
     1801 gtcacctccc gcgccgcgtc gcggcgcctc cccagcgccg tggccgccgc cgcccgcgcc
     1861 ttgtagctgt cgtccccctc cagggcgcgg cggagcaacg gcagcgactc ccggtcccgc
     1921 gggaagtttc ccagagacgc gagcaccatg gtcctgaccc ggctgtcgcg gtcgccggct
     1981 ccggcctcca gagcctggcg cgccgcctcc gtcccggcct ggcccagggc ctcggcggcg
     2041 acctgccgcg ccccatggaa cgggtcctgg cgcagcgcct tcgccagggc ctcgaccgcc
     2101 ggcgcgtccg ccagctctcc cagggcgcgc aacgcctcca gacgggcggg cgtctcggcg
     2161 gcgcccgccg ctgccgagag ctgggccacc cactccgccg tcggcttgtc gaagtcgacg
     2221 gccgcgagga cccagtcccg cggatcgaag accacggtcc gcggccgggc cgggctgtgg
     2281 agatagatct cctgctccgc ccgggcgagg atctgcaccg tctggatctc caggccaccg
     2341 tctccgacgt cgccggccag cccgatctcg accgggaagg agaagaggcc ggtctcgccg
     2401 gtgacctcct gggtctgccg cacctccagg cgcaccagct cgcggtccgc ctggtagtcc
     2461 cagcggacct cgagctccgg atgaccggcg ccgtagacgt attgatcgaa cagcggcccc
     2521 agggtgacgc ccgtcgactt ttccaggacg ctctggaagt cggaggtctt cacggtctgc
     2581 agggcgaagc ggtcgagata ggcgcggatc cccttccacc acccctcttc accgaggagc
     2641 ccgtggatca tgcgcatcac cagcgccccc ttctcatagg agtggcggtc gaacagggtg
     2701 atgggatcgg ggtagcggtc ggtgacgatg gggcgcctgt accggcgggc gtcctcccgc
     2761 aggtaggcct ggcggatggt ttccagctcc acggcggcct cctccgcgcc gccggcatgc
     2821 cccaggtaga ggacggtgag gtagtcggcc atcccctcgt tgagccagag gtgcgaccag
     2881 tcctcgcagg tgacgaggtc gccgaaccac tggtgcgcca gctcgtgggc caccagcgag
     2941 tcgctccgcg agtcgttgtg ggcccgctcg tcgtgcaggg tgcggagcgt ctgggtggtg
     3001 gcggtgatgt tctccatgcc gccccaggtg aagtccgcca cggcggtctg ggcgtacttg
     3061 gcgtaggggt acggccggcc ggtgacggtg gagaagtact ccatcatctc gggggtcttg
     3121 ccgaaggagc ggcgcgcccg gtcggcccag cgcggcggca cgtag
//
LOCUS       DASPJI010000332        11292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_534253, whole genome shotgun sequence.
ACCESSION   DASPJI010000332 DASPJI010000000
VERSION     DASPJI010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11292)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11292
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534253"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..5583
                     /locus_tag="VEL74_08800"
     CDS             157..5583
                     /locus_tag="VEL74_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62667.1"
                     /translation="MSQSEIEFAYELSPIQKGMLFHSAYASGSGVYVVQISLRLTGKL
                     DIGAFERAWRHVMDRHDILRAGFYWEDLEKPVQVVYRDVPLEIERASLRSFDPGEQRV
                     RLQRFMDEEREHGFALDEPPLMRLALFELDEGVHHFVWSQHHLLTDGWSQGLVLKELF
                     VCYAAFAGGREPSLGRARPFRDYIAWLQRQDLARAEAAWRRSLAGFSAPTFLAQGGER
                     EADEVEFQDSRRRGLRLPAEATAALREVARRHRLTLNTLVQAAWAIVLGRLTGTRDVV
                     FGATGAGRPTDLPGVESIAGPFINTLPVRVTMAADSGLVSWLARLQEQQVELRLYEYA
                     PLYEVQKWSEVPPGVALFDNILVFENYPFDQALTDLLPDLAISQVVANELTNYPLNVV
                     VFADAELALDILYDSGRFETVAVVRMLEHLSALLRTFATGSDMKVWQLPLLLAGERHQ
                     ILAEWNDTAEGWPAEASLHGLFERQVEQSPDAVAAWFEEAGISYAELDRRADRLARHL
                     ARADRGIYVALCMERSAEMLIALLGILKAGLAYAPLEPAWPADRKRWILDSLNVRCAV
                     TGTSQLRAMHDLQWQLPALAELIVLDAGDRRPPAEPVVADAVRRLWDHVAERAVDRVS
                     AGGFVSSYTGRPFSEAEVNEYRDRVVSLASPWIRPDARVLEIGCGSGLILFELAPRVG
                     RYVGLDPSGLTQERNRREAAARGLANVELPTAFAHELEAAGEGPFDLVILASTVHFFP
                     GPFYLERVLAAAFERLAPGGAVLLADVPDLRRREEYRASLDAFRAAHRGEEGLRTRSR
                     IDDLYLDEDFFSDLAAGLPAAAEVLHRRQGFDNELRFRYDVLLRRPAAPTPRPAQRHK
                     EVRTLWHLDGPGAPARVPALPPVSADALAYVIFTSGSTGTPKGVVVRHRPAVNLVDWV
                     NRTFEVGPRDRVLFVTSPCFDLSVYDVFGLLAAGGSLRIASDSDLRNPERLVRWLVED
                     GVTFWDSAPAALQQLVPFLPQGSGGGRGLRLVFLSGDWIPLPLPESMSASFPAARVIG
                     LGGATEATIWSNYFPVVALDPRWTSIPYGRPINNAVYYVLDSGLEPCPVGVAGDLYIG
                     GECLASGYAGDPALTASKFLPDPWSVLPGGRRYRTGDLARFFADGNIEFLGRADHQVK
                     IRGYRIELGEIETVLASHPAIRESVVAVREDTPGARRLVAYVVGSDAAEGELRAFLHE
                     KLPDYMVPAAYVVLDAMPVTANGKLDRKALPAPQKAGSEGDGAGPRTAAEVTLAEIWS
                     QVLGVERVGVRDNFFELGGDSILAIQIVFRARQEGLWLDPKDLFRHQTLGDLAAAAGA
                     ARAESADEEDGGDIPLLPIQRWFLELGLRDAHHFNHAVLLQPREELAPALLAGALDTI
                     AAHHVALRVRFLPGEGGQWRQTVEGASSVLAFLDLSALPAALRPAALEAAAAHLQPSL
                     DLERGPVWRAVLFSLGEGGQRLLLLLHHLIVDGVSWRILLEDLDLAYRTLARERRPVL
                     PPVATSLRGWATRLVVHARSAAVAQEASYWLSGARAAVLPLPLDWPEGDNLVASARTV
                     FAELDEDETHALLHEVPAVYHTRINDVLLTALAQAFADWTGESRLLVDLEGHGRVDLF
                     EGVDTSRTMGWLTSLFPVLLEVSGRDPGERLKAIKEQLRSVPGEGIGHGLLRYLGDKG
                     TADLLRDMPIADVSFNYLGQLDRVLADSSLFAPATETAGPARSPYQARHHLLEINGGV
                     SGGRLRMGWTYSVSLHDSYTVESLAERFLASLRELIEHCRLEEAGGFTPSDFPEARAS
                     QKELDKLMAQIDKRGPGAVAPPSR"
     gene            5606..>11292
                     /locus_tag="VEL74_08805"
     CDS             5606..>11292
                     /locus_tag="VEL74_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62668.1"
                     /translation="MSRKDVELVYELSPVQQGMLFQTVYAPGSGVYVIQLSLRLTGRL
                     HLAAFERAWRQVVARHAILRTAFYWEDLEKPLQVVYRQVDVRLTRQSWRGLGADEQRD
                     RLARYLEADRERGFDLAQPPLMRLALFDLGDGAHQFVWSHHHLLSDGWSQGLLLRELF
                     TLYAALADGQDPALGEPRPYRDYIGWLQRQDLREAEVFWRRNLAGLSQPTLLADGDTR
                     QAAAADYRSSRGRGFLLGREETAALRDMARRRRLTLNTVVQGAWGLLLARSLGAREAV
                     FGSTVAGRPADLPGADSIIGPFINTLPVRVAVAPEHRLVPWLTGLQERQLELRRHDYV
                     PLYQVQRWSELPAGTPLFDSFLAFENYPIDSTLGALLPGLDFSEARTNDLTDYPLALV
                     VLPGNELSFESRYDASRLEDATVARLLERLAGLLRSLPAASGVTLGELPLLLPAERHQ
                     VLREWNDAATALASAGPGRCLHELVWARAAAAPDTLAVECEGRTLSYSELERRSRQLA
                     SHLAGLGARPDSRVGVLLERSPEMVVALLGVLEAGAAYVPLDPVHPAERLAYLAGDSG
                     VSLLLAPGAVPEGLRGGGFAILEAESWDGVAGGSEEPARGPWPESLAYVIYTSGSTGE
                     PKGVGVSHEVTAAHMGVAARAYGIGPRDRILQFSSFAFDAGLEQVFTALTQGATLVLR
                     GEQAWDTADLTERLARLEVTVANFPSSYWERWTAVLPAGNGSGPEPPLRLMIAGGEAM
                     LPEWAERWQVSRFGAVRLLNAYGPTEASVTAAFEKVTWTSASRAAVPIGRCVAGRAAY
                     VLDEGLEPVPPGAPGELCLGGPLLARGYLGRPGPTAAAFVPDPFAATSGARLYRTGDR
                     VRALPDGRLEFLGRVDRQVKVRGFRVEPGEIEAALERHSGVREAAVDLRGDLPGGSAL
                     VAYVAASVSATDLRSFLSDNLPAPLVPGHFVILDELPRTPGAKVDRHALSRLEVRPDG
                     QLADGADAAALHPPRNPVEALLAGIWQSVLGIPSVGIHDDFLALGGHSLLAMQLLSRV
                     RETFRIDLPLASLFEAPTISGQAELVEQRLRTGTGLAQPPLVPMPRQGDLPLSFAQQR
                     LWFLEQLQPGGAAYNLPTAVRLTGDLPVPLLARIFAEIVRRHESLRTVFVAEEDGRED
                     PAQRIAPPQDVDLPVVELAGLAEADREAEARRLAGEEAWRPFDLATGPLLRLTLLRLA
                     AREHVLLMTMHHIVSDGWSAGVLLREIAALFEAFSAGRPSPLPELPVQYADFASWQRS
                     WLQGEVLESQLSYWRHQLAGAPRVLDLPLDRPRPAVQSQQGVWWPVALSPELSERVRQ
                     LCRGTGMTPFMALMAGWALLLGRHAGQQEVLVGTPIAGRNRREIEGLIGYFVNTLVLR
                     TDLAGNPIFGELLERVREASLGAYTHQDVPFERLVEELVPDRDLSRSPLFQVMLVLQN
                     DGGPGLSLPGLTLSPVPLRGQVAKFDLTLTLQEEERFRGGLEYDTSLFDGSTVTRLWA
                     RFENLLEGAVHAPDRPLGDLPLLSVAERHQALVEWNDTRRPAGDLGRLPLAHELIAIQ
                     ARRSPHALAVIAGDRRLSYGDLDAQANRLAWELVGRGVGPDVAVALFLERSAELVIGL
                     LAILKAGGAFVPLDPGHAGERLAFTLSDSGAALVLTRRALLASLPALPGVMPLLLDEP
                     PQVPTVNGEPVSPPGGAKSENLAYVLYTSGSTGRPKGVMVPHQGLANYLAWCLQAYPL
                     AEGSGAPVHSSVAFDLTITSLLAPLAGGKSVRLLPESPGGEALTEAFAEAAVGGERYS
                     LIKITPAHLELLSTQLRDVTAAGGGAACLVIGGEALTGAALSFWQAHAPRTRLINEYG
                     PTETVVGCSVYEVPLGVPFANAVPIGRPIADTRIYLLEPGMEPTLPGSRGELF"
BASE COUNT         1428 a         3918 c         4104 g         1842 t
ORIGIN      
        1 ccggtcggag gccggcccga tgagctttgt cctggccctc ctcgtggctc tggtgatcgc
       61 ttggctggcg gcgagctacg gggcgctgcg ggcggcccgt gacgagccgt cactcgaagc
      121 tgcggggcga ggggcggagg agctgcggag ggcgatgtga gccagagcga gatcgagttt
      181 gcctacgagc tgtccccgat ccagaagggg atgctgttcc acagcgcgta tgcctccggc
      241 tcgggcgtct acgtcgtcca gatcagcctg cggctgaccg gcaagctcga tatcggcgcc
      301 ttcgagcggg cgtggcggca cgtgatggac cgtcacgaca tcctgcgcgc cggcttctac
      361 tgggaggacc tggagaagcc ggtgcaggtg gtctatcgcg acgtccccct ggagatcgag
      421 cgcgcgtccc tgcgcagctt cgacccgggg gagcagcgcg tccggctgca gcgcttcatg
      481 gatgaagagc gggagcacgg cttcgcgctc gacgagccgc ccctgatgcg gctggcgctg
      541 ttcgagctgg acgagggggt ccaccacttc gtctggagcc agcaccacct gctgacggac
      601 ggctggtccc aggggctggt cctcaaggag ctgttcgtct gctacgcggc gttcgccggc
      661 ggccgggagc cgtcgctggg ccgggcgcgc cccttccgcg actacatcgc ctggctccag
      721 cggcaggacc tcgccagggc cgaagccgcc tggcggcgga gcctcgcggg gttctccgcc
      781 cccaccttcc tggcgcaggg gggcgagcgc gaggcggacg aggtcgagtt ccaggattcg
      841 cggcggcgcg ggctgcggct tcccgccgag gccaccgccg ccctgcgcga ggtggcgcgg
      901 cgccaccggc tgacgctgaa cacgctggtg caggcggcct gggcgatcgt gctgggccgc
      961 ctgaccggca cgcgggacgt ggtcttcggc gccaccggcg cgggccggcc gaccgacctg
     1021 ccgggcgtcg agtccatcgc cggccccttc atcaataccc tgccggtgcg ggtgacgatg
     1081 gcggcggaca gcggcctcgt ctcctggctg gccaggctgc aggagcagca ggtcgagctg
     1141 cgcctctacg aatacgctcc gctctacgag gtccagaagt ggagcgaggt gccgccgggc
     1201 gtggcgctgt tcgacaacat cctggtcttc gagaactacc ccttcgacca ggcgctcacc
     1261 gatctcctgc cggacctcgc gatctcgcag gtggtggcga acgagctgac gaactacccg
     1321 ctgaacgtgg tggtcttcgc cgacgccgag ctggcgctcg acatcctcta cgacagcggc
     1381 cgcttcgaaa ccgtggccgt ggtccgcatg ctcgagcacc tctcggccct gctgcggacc
     1441 tttgcaaccg gttccgacat gaaggtgtgg cagttgccgc tgctgctcgc gggcgagcgc
     1501 caccagatcc tggcggagtg gaacgacacg gcggagggct ggcccgcgga ggcctctctc
     1561 cacggcctgt tcgagcggca ggtggagcag tcgcccgacg cggtggccgc ctggttcgag
     1621 gaggcgggga tcagctatgc cgagctggac cgccgggcgg accgtctcgc ccggcatctc
     1681 gcccgagccg accgggggat ctacgtggcg ctctgcatgg agcgctcggc cgagatgctc
     1741 atcgccctcc tcggcatcct caaggcgggg ctggcgtacg ccccgctcga gcccgcctgg
     1801 ccggcggacc gcaagcggtg gatcctggac tcgctgaacg tccgctgcgc cgtgaccggc
     1861 acctcgcagc tccgggcgat gcacgacctg cagtggcagc tcccggcgct cgccgagctg
     1921 atcgtcctcg atgccgggga ccgccgtccg ccggccgagc ccgtggtggc cgacgcggtg
     1981 cgccggctct gggaccacgt ggcggagcgc gcggtggacc gtgtcagcgc cggcggcttc
     2041 gtcagcagct acaccggccg gcccttctcc gaggccgaag tgaacgagta ccgcgaccgg
     2101 gtggtctccc tggcgtcgcc ctggatccgg ccggacgccc gggtgctgga gatcggctgc
     2161 ggctccggtc tgatcctctt cgagctcgcc ccccgggtcg gccgctacgt cggcctcgac
     2221 ccgtccgggc tgacgcagga gcgcaaccgc cgcgaggccg ctgcgcgcgg gctcgccaac
     2281 gtggagctcc ccaccgcttt cgcccatgag ctggaagcgg ccggtgaggg tccgttcgac
     2341 ctggtgatcc tcgccagcac ggtgcacttc ttcccgggcc cgttctatct cgagcgggtc
     2401 ctggccgccg ccttcgagcg cctcgccccg ggtggcgccg tgctgctggc tgacgtgccc
     2461 gacctgcgcc gccgcgagga gtatcgcgcc tctcttgacg ctttccgtgc cgcccaccgc
     2521 ggtgaggagg ggctgcgcac ccgctcccgg atcgacgatc tgtacctgga cgaggacttc
     2581 ttcagcgatc tggcggccgg gttgcccgcc gccgcagagg tcctccatcg ccggcagggc
     2641 ttcgacaacg agctgcgctt ccggtatgac gtgctgctgc ggcggccggc ggcgcccacg
     2701 ccgcggccgg cgcagcgtca caaggaggtg cggacgcttt ggcacctgga cggccccggc
     2761 gcccccgcca gggtgccggc cctcccgccc gtctctgccg acgccctggc ctacgtcatc
     2821 ttcacttcgg gctccaccgg tacgcccaag ggggtcgtgg tgcgccaccg gcccgccgtc
     2881 aatctggtgg actgggtcaa ccgcacgttc gaggtcgggc cacgtgaccg agtcctgttc
     2941 gtgacctccc cctgcttcga cctgtcggtc tacgacgtct tcggcctgct ggcggccggc
     3001 ggctcgctgc ggatcgccag cgactctgac ctcaggaacc cggagcgtct ggtgcggtgg
     3061 ctggtggagg acggcgtcac cttctgggac tcggcgccag cggccctgca gcagctcgtc
     3121 cccttcctgc cgcaggggag cggagggggc aggggcctgc gtctggtctt cctgagcggc
     3181 gactggatcc ccctccccct gccggagagt atgagcgcga gcttcccggc agctcgggtg
     3241 atcggcctgg gcggcgccac cgaggcgacg atctggtcca actacttccc cgtggttgcg
     3301 ctggatcccc gctggaccag catcccctac ggcaggccca tcaacaacgc cgtctactac
     3361 gtgctcgact ccgggctcga gccctgtccg gtgggtgtgg ccggcgacct gtacatcggc
     3421 ggcgagtgcc tggcctccgg ctatgccggc gatccggcgc tgaccgcctc caagttcctg
     3481 cccgatccgt ggagcgtcct gccgggaggc cggcgctacc gcaccggtga cttggcgcgc
     3541 ttcttcgccg acggcaacat cgagttcctc gggcgcgccg accaccaggt caagatccgc
     3601 ggctaccgca tcgagctggg ggagatcgag acggtgctgg cgagccatcc ggcgatacgg
     3661 gagtcggtcg tcgccgtccg cgaggacacg ccgggggccc gccgtctggt ggcctacgtt
     3721 gtgggctctg atgccgcgga aggcgagctg cgcgctttcc tgcacgagaa gctgcccgac
     3781 tacatggtgc cggcggccta cgtggtcctg gatgccatgc cggtgacggc caacggcaag
     3841 ctcgatcgca aggccctgcc ggcgccgcag aaggcggggt cggagggtga cggggcaggc
     3901 ccgcggacgg cggccgaggt caccttggcg gagatctggt cacaggtgct gggggtcgag
     3961 cgcgtcgggg tccgcgacaa cttcttcgag ctgggcggtg actccatcct ggcgatccag
     4021 atcgtcttcc gcgcgcggca ggaggggttg tggctcgatc ccaaggacct cttccggcac
     4081 cagaccctgg gcgacctggc cgccgccgcg ggggcggcac gggcggagtc cgcggacgag
     4141 gaggatggtg gggatatccc tctgttgccg atccagcgct ggttcctcga gctcggcctc
     4201 cgcgacgcgc atcacttcaa tcacgcggtg ctcctccagc cgcgggagga gctggccccc
     4261 gccctcctcg ccggtgcgct ggacaccatt gccgcccatc acgtagccct gcgcgtccgc
     4321 ttcctgcccg gagagggggg gcagtggcgc cagacggtgg agggggcgtc gtcggttctg
     4381 gcattcctgg acctctccgc gctgccggcc gctctgcggc cggcagcgct ggaagcggcg
     4441 gccgcccacc tccagccgag cctcgacctg gagcgcggcc cggtgtggcg ggcggtgctg
     4501 ttctcgctcg gggagggcgg acagcggctg ctgctgctgc tccatcacct catcgtggac
     4561 ggggtctcct ggcgcatcct gctggaggat ctcgacctgg cctaccggac gctggcacgg
     4621 gaacggcggc cggtgctgcc gccggtggcc acctcgctgc gcggctgggc aacgcgtctc
     4681 gtcgtgcacg cccgttcagc ggccgtggcg caggaggcga gctactggtt gtcgggcgcc
     4741 cgcgccgcgg tcctgccgct gccgctcgac tggccggagg gggacaacct ggtggcctcg
     4801 gcccgcacgg tattcgcgga gctggacgag gacgagaccc acgcgctcct gcacgaggtg
     4861 ccggccgttt accacacgcg gatcaacgac gtgctgctga ccgcgctggc gcaggccttt
     4921 gcggactgga ccggtgagtc gcgcctgctg gtggacctgg aggggcacgg ccgggtggac
     4981 ctgttcgagg gtgtggacac gtcgcgcacg atggggtggc tcacgtccct gttcccggtg
     5041 ctcctggagg tctccggccg cgatccgggg gagcggctca aggcgatcaa ggagcagttg
     5101 cggtcggtgc ccggagaggg gatcggccat ggcctgctgc gctatctggg ggacaagggg
     5161 acggcggacc tcctgcgcga catgcccatc gccgacgtga gcttcaacta tctgggccag
     5221 ttggatcggg tgctggcgga ctcctccctg ttcgccccgg ccacggagac ggcagggccg
     5281 gcgcggagcc cataccaggc gcgccaccac ctgttggaga tcaacggcgg ggtgtccggt
     5341 gggcgtctgc gcatgggctg gacctacagc gtgagcctcc acgattccta taccgtcgag
     5401 tctctggcag agcgcttcct tgcctcgttg cgggagctga tcgagcactg ccgtttggag
     5461 gaggccgggg gcttcacgcc gtcggacttc ccggaggcgc gcgccagcca gaaggaactc
     5521 gacaagctga tggcccagat cgacaagcga ggcccgggag cggtggcgcc cccgagccgc
     5581 tgagacctgc cgtcgcgagg ccgtcatgag tcgcaaggac gtcgagctcg tctacgagct
     5641 gtcccccgtt cagcagggga tgctcttcca gaccgtctat gccccaggct cgggcgtcta
     5701 cgtcatacaa ctgagcctgc gtcttacggg caggctgcat ctcgccgcct tcgagcgggc
     5761 gtggcgccag gtcgtcgccc ggcacgccat cctgcgcacc gccttctatt gggaggacct
     5821 cgagaagccg ctgcaggtcg tctaccggca ggtggacgtg aggctgacgc ggcaatcgtg
     5881 gcgcgggctt ggcgcggacg agcagcgcga ccggctcgcg cgctacctgg aggccgaccg
     5941 ggagcgcggg ttcgatcttg cccagccgcc cctgatgcgg ctggcgctgt tcgacctcgg
     6001 agatggggcg caccagttcg tctggagtca tcaccacctg ttgagcgacg gctggtccca
     6061 gggccttctc ctgcgcgagc tgttcaccct ctacgcggca cttgcggacg gccaggatcc
     6121 ggctctcggc gagccgcggc cctatcgcga ttacatcgga tggctccagc ggcaggacct
     6181 gagggaggcg gaggtcttct ggcgccgcaa cctcgcgggg ctctcccaac ccaccctcct
     6241 ggcggacggc gacactcgtc aggcggcggc agcggactac cgcagctccc ggggccgcgg
     6301 cttcctgctt ggccgggagg agaccgcggc tctccgcgac atggcgcgac gccggcggct
     6361 gacgctgaac acggtggttc agggcgcctg ggggctcctg ctggcgcgct cgcttggggc
     6421 ccgcgaggcg gtcttcggga gcacggtggc gggccgaccg gcggaccttc cgggagcgga
     6481 ctccatcatc ggccccttca tcaacacgct gccggtgcgg gtggctgtgg cgccggaaca
     6541 ccggctcgtg ccctggctga ccgggctcca ggagcggcaa ctcgagctcc gccgccatga
     6601 ctacgtgccc ctttaccagg tgcagaggtg gagcgagctg cccgcgggca ctccgctgtt
     6661 cgacagcttc ctggcattcg agaactaccc catcgactcg actctcggcg cgctgcttcc
     6721 agggctcgac ttctccgagg cgaggaccaa cgacctgacg gactatccgc tcgccctggt
     6781 agtcctgcca ggaaacgagc tgtcgttcga gagccgctac gacgcgagcc gcctggaaga
     6841 cgccaccgta gcccgcctgc tcgagcgcct cgcggggctc ctgcgtagcc tcccggccgc
     6901 ctcgggggtg accctggggg agctgccgct gctgctgccg gcggagcggc accaggttct
     6961 gcgcgagtgg aacgacgcgg cgaccgcgct ggcgtcggcc ggtccgggcc gctgtctgca
     7021 cgagctggtc tgggcccggg ccgcggcagc gccggatacg ctcgccgtgg agtgcgaggg
     7081 ccggacgctg agctatagcg agctggagcg gcggtcccgt cagctggcga gccacctcgc
     7141 cggcctgggt gcccggccgg attccagggt gggcgtgctc ctcgagcgct cgccggagat
     7201 ggtcgtcgcg ctactcggcg tgttggaggc cggggcggcc tacgtgccgc tggacccggt
     7261 gcatccggcc gagcggctgg cctacctggc cggtgactcc ggcgtgtcgc tgctgctcgc
     7321 gccgggcgcg gtacccgagg ggctgcgcgg cggcggtttt gcgatcctgg aggcggagtc
     7381 ctgggatggg gtggcggggg gctctgagga gccggcgcgc gggccctggc cggaatccct
     7441 ggcctatgtc atctacacct cgggatcgac cggcgagccc aagggggtgg gagtctccca
     7501 tgaggtgacc gccgcgcaca tgggggtggc cgcgcgggcc tacggcatcg gcccgcgcga
     7561 ccggatcctg cagttctcct cgttcgcgtt cgacgccggg ttggagcagg tcttcaccgc
     7621 cctcacgcaa ggcgcgacgc tggtgctgcg cggcgagcag gcctgggata cggcggacct
     7681 caccgagcgc cttgcgaggc tggaggtgac cgttgccaat tttcccagct cgtactggga
     7741 gcggtggacg gcggtgttgc cggcgggcaa cggaagcggt ccggagccgc ccctgcggct
     7801 gatgatcgcc ggcggcgagg cgatgctccc cgagtgggcc gagcgctggc aggtctcgcg
     7861 gttcggcgcc gtccgcctgc tcaacgccta tggaccgacg gaggcatccg tcaccgcggc
     7921 cttcgaaaaa gtgacctgga cgtccgcctc ccgcgccgcc gtacccatcg gccggtgcgt
     7981 cgctgggcgc gctgcctacg tcctcgacga ggggctcgag cccgttcccc ctggagctcc
     8041 gggtgagctg tgcctgggcg gtccgctcct cgcccgcggc tatctcggcc gtcctggccc
     8101 gaccgccgcg gctttcgtgc ccgatccctt cgccgcaacc tccggagccc ggctctaccg
     8161 caccggcgat cgggtgcggg cactgccgga tggacgtctg gagttcctcg ggcgcgtcga
     8221 ccgccaagtc aaggtgcgcg gcttccgtgt cgagcccggc gagatcgaag ccgccctcga
     8281 gcgccattcc ggtgtgcgcg aggccgctgt ggacctgcgg ggcgatctgc ccggcggctc
     8341 ggcgctggtg gcgtacgtgg ccgcgtccgt ctctgcgacc gatctccgca gcttcctctc
     8401 ggacaacctc ccggcacccc tggttccggg acacttcgtg attctcgacg agctgccgcg
     8461 gacaccggga gccaaggtcg accggcatgc cctgtcgcgt ctggaggtcc ggccggacgg
     8521 ccagctcgct gacggagccg atgccgcggc cctccatccg cctcgcaatc cggtcgaggc
     8581 tctgctcgcc ggaatctggc agagcgtcct ggggatccca tccgtcggca tccatgacga
     8641 cttcctggcg ctgggcggcc actccctgct ggccatgcag ctgctgtcac gggtgcgcga
     8701 gacgttccgg atcgatctgc cgctcgcgtc gctattcgaa gcgccgacga tctctggcca
     8761 agccgagctc gtggagcagc gcctgcggac cggaacgggc ctcgctcagc cgccgctcgt
     8821 cccgatgccg cggcagggag acctgccgtt gtcgttcgcc cagcagcggc tctggttcct
     8881 ggagcagctc caaccgggcg gcgctgccta caacctcccc acggccgtgc gcctgacggg
     8941 agacctcccg gtgccgttgc tggcgcggat cttcgccgag atcgtgcgcc ggcacgagtc
     9001 gctacggacc gtgttcgtgg cagaggagga cggccgggaa gatccggcgc agaggatcgc
     9061 tccgccacag gacgtcgatc tgccggtcgt ggagctggcg ggcctggcgg aggcggatcg
     9121 ggaggccgag gcgcgccggc tggccggaga ggaggcttgg cgaccgttcg acctcgcgac
     9181 cgggccgctg ctgcggttga cgctgctgcg cttggccgcg cgcgagcacg tgctgctgat
     9241 gaccatgcac cacatcgtct cggatggctg gtccgcgggt gtgctgctgc gtgagatcgc
     9301 ggcactgttc gaggcttttt cggcgggccg tccttcgccg ctgcccgagc tgccggttca
     9361 gtacgccgat ttcgcctcct ggcagaggag ctggctgcag ggggaggtgc tggagagcca
     9421 gctctcctac tggcggcacc agctcgccgg ggctccgcga gtgctcgatc tgccgctgga
     9481 ccgtcctcgc ccggcggtgc agagccagca gggcgtttgg tggccggtgg ccctctctcc
     9541 ggaattgtcg gagcgggtgc gccagctctg ccgcggcacc ggaatgacgc cgttcatggc
     9601 gctgatggcg ggctgggcgc tgctgctggg ccgccacgcg ggccagcagg aggtgctggt
     9661 cggtactccg atcgcgggac gcaatcgccg tgagatcgag gggctgatcg gctacttcgt
     9721 caacaccctg gtgctgagga ccgacttggc gggcaatccg atctttggtg agctgctgga
     9781 aagggttcgg gaggcctctc tgggcgccta cacgcaccag gacgtgccgt tcgagcggct
     9841 ggttgaggag ctggtgccgg atcgcgacct gtcccgctcg ccgctgttcc aggtgatgct
     9901 cgtcttgcag aacgacggtg gcccgggact gtcgttgccg gggctcacgc tctcgccggt
     9961 gcccctgcga gggcaggtgg cgaagttcga cctgacgttg actctccagg aggaggagcg
    10021 gttccggggt gggctcgagt acgacacgag tctgttcgac ggcagcaccg taacgcggct
    10081 gtgggctcgc ttcgagaatc tgctggaggg ggcagtccac gcgcccgacc ggcccctcgg
    10141 ggacctcccg ctcctctccg tggccgaacg ccatcaggcg ctggtcgaat ggaacgacac
    10201 ccggcgtcct gcaggagacc tcgggcgact gcctctggcc cacgagctca tcgccatcca
    10261 ggcccgccgc tcgccgcacg cgctggcggt gatcgcgggc gaccggcggc tgagctatgg
    10321 ggatctcgac gcgcaggcga accggctggc gtgggagctg gtagggcgcg gcgtcgggcc
    10381 cgacgtggcg gtagccctgt ttctggagcg ctccgccgag ctggtcatcg gcctgctcgc
    10441 catcctcaag gccggagggg ccttcgttcc cctcgacccg gggcatgccg gagagcggct
    10501 cgccttcacc ctgagcgact cgggcgcggc tctcgtcctc acccggcggg ctctcctggc
    10561 ttcattgcct gccctccctg gcgtgatgcc gctgctgctc gacgagcctc cccaggtgcc
    10621 tacagtcaat ggtgagccgg tcagcccccc tggaggcgcc aagtcagaga acctggccta
    10681 tgtcctgtac acctccggct cgacgggcag gccgaagggg gtgatggtgc cgcaccaggg
    10741 gctggccaac tacctggcct ggtgtctcca ggcgtacccc ctggccgagg gctcgggcgc
    10801 gccggtccat tcttcggtgg cgttcgatct gaccatcacc agtctcctcg caccgcttgc
    10861 cgggggaaag agcgtccgcc tgctgcccga atcgccaggg ggcgaggcct tgaccgaagc
    10921 gttcgcggag gcggcggtcg gcggcgagcg gtacagcctg atcaagatca cgccggcgca
    10981 tctggagctg ctgtccactc agctccggga tgtgactgcg gccggtggcg gtgccgcttg
    11041 tctggtgatt ggcggcgagg cgctcaccgg agccgcgctg tcgttctggc aggcgcacgc
    11101 tcccaggacc cggctgatca acgagtacgg gccgacggag accgtcgtcg gctgctccgt
    11161 ctatgaggtg cctctcggag tccccttcgc caacgctgtc cccatcggac ggccgatcgc
    11221 cgatacccgg atctatctgc tggagcccgg catggagccg acgctccccg ggtcgcgggg
    11281 agagctgttc at
//
LOCUS       DASPJI010000333         5979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_591546, whole genome shotgun sequence.
ACCESSION   DASPJI010000333 DASPJI010000000
VERSION     DASPJI010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5979)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591546"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VEL74_08810"
     CDS             complement(<1..964)
                     /locus_tag="VEL74_08810"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYG62669.1"
                     /translation="MRIFVTGATGYIGRTLAQRLVEGGHEVRVVVRATSRVEPLHELG
                     VTTFTGDIGDRYSLREGMSGADWVIHAAAVLDPDAPPERMREANVQGSENVASLAYKL
                     GVPRFLSVSSIAYFGGSPEDGSAATETSPPLQPFPTLYSATKHSGERAIREWGRRGLA
                     VNTVYPSLVYGPPGKKEGANALLRALLRGRFPALVGADRKSCWVYLEDVVDGMLRVIE
                     RAPVGRDYLLAGEIVTLRELVERVCALGGVKPPRLSLPVGLARVGLKASGPLFRMRGR
                     RPPFSTEQLGSLERHWAFDDSRARTELDWHPRGLAEGMPPTVAFL"
     gene            complement(1211..1966)
                     /locus_tag="VEL74_08815"
     CDS             complement(1211..1966)
                     /locus_tag="VEL74_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HYG62670.1"
                     /translation="MSVTIATWNVNGIRAREAQFLEWVGRDRPDIICLQEIKASPSQL
                     SETLCGLEGYSCYWHGAGGYSGVSLNVRDGLSPEPPRYSHPAFDHETRIVTAEIGDLV
                     VASVYVPNGGKDFAAKMRFLESLREYAAAMRAEGRRLVICGDLNVARTDQDLHPKERK
                     PGLIGQRPEERELFEAILAEDLVDVGRHLDPDNDGLFTWWAPWRNMRQRNIGWRIDYI
                     LASRPVAERATASRVLADVGTSDHAPVVATFDL"
     gene            complement(2030..2419)
                     /locus_tag="VEL74_08820"
     CDS             complement(2030..2419)
                     /locus_tag="VEL74_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG62671.1"
                     /translation="MRFLLDTCVLLWWVGDPARLSGPARELLLQPDLTLYVSAISAFE
                     ISIKHRKGKLQLPLPPRQWFLRALSAYPLTELPVTSEVAGLAPEVDAPHSDPCDRIIM
                     ATAQIHGIPILTPDSLIAAAREVTVIW"
     gene            complement(2423..2668)
                     /locus_tag="VEL74_08825"
     CDS             complement(2423..2668)
                     /locus_tag="VEL74_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62672.1"
                     /translation="MPMHAHKLKVNVPPDHQIEIHLPEDFPQGPAEVIVLASVAERRS
                     GAREAGIRSLEPHPLLGKIIFHEDPSLPLDAEDWPEE"
     gene            2765..5185
                     /locus_tag="VEL74_08830"
     CDS             2765..5185
                     /locus_tag="VEL74_08830"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62673.1"
                     /translation="MSSLRSALRAASVALAAACLAAPAFSAPVPRREREALIDFYRAT
                     GGDAWGRRDRWLGAPGTECSWYGVSCDASGSVASLVLNTNGLTGGVPASIAGLENVRT
                     LVLRENRLASLPADLGRLQRLASLDVEANALRGLPPELGQLQGLRFLRLSRNPLGALP
                     GETLGALSSLESLYADGTGLSELPAEIGRLEKLRFLSLRDNGLTALPAETGSLRELLL
                     LDLVNNRLETLGPEIGRLVALTTLVADGNLLEALPPEIWRLPALTSLSLPGNRLSSLP
                     PLPPETTPEPALLYLDLSGNRFTTFPADVGRLRSLTTLRLADNQIAEVSGILGDLPAI
                     ERLDLSRNLLTAVPAGLVRLGTLESLLLAGNRISAVPAELAALGQLFSLDLSDNRIAA
                     LPPELSRMAALRWLLLPDNLLADLPAELGGLGLEFLDLAGNHLETLPAAVLGMASLEI
                     LNLSSNRIWGDIPRELAGLERLELLYLSGNPLTPGPIPDWLRGLPLRGLGLRATNRTG
                     EIPAWLGDSRLESLDLGQNPFDPAPVPDSLRRLTTLRSLWLDHTLRLGAIPAIQEWLG
                     ARDLVHLSLASNPFTPGPIPDLSGSRNLFLLDLGETNRTGSLPEWLAGARSLGYLLLE
                     RNRLEGGIPESWGALTSLRTLWLRSNRLTGQVPAALTALGLLEERAGLDLRWNSLRQS
                     EPGTLAFLNAKQAGRVDFRDTQTLTPRGVAARSLGPDSVLLTWDPIRYRADEGYYAVF
                     KATAPEGPYTLAGRTASKHDTALEVIGLTPRTVYWFIVRAVTEPHAENANRVVSPPTP
                     PVRARTFP"
     gene            complement(5142..5798)
                     /locus_tag="VEL74_08835"
     CDS             complement(5142..5798)
                     /locus_tag="VEL74_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62674.1"
                     /translation="MAPLLLRRLTLSAALLTLAACGSSEEAELDVAPPLATARISSYT
                     GWFPGPWRQEIWIRLDEGVELSEVAQLSVFGKLRPGMSLGEAVAVAGRPAGQSTDPRG
                     TRWSHWFSGSDSVQVGCVESCSGDDCFSSWELRAQGSGLSPEGIFIPAIVEILQQAEA
                     SRPKVGYRAIHVATQDASHRLTLELPSRRGRALSWGGEMAPCESPKGTSAPGRAASGG
                     "
BASE COUNT          870 a         2194 c         2033 g          882 t
ORIGIN      
        1 ggaggaaggc gacggtcggc ggcatcccct cggcgaggcc gcgggggtgc cagtccagct
       61 cggtccgggc gcgggagtcg tcgaacgccc agtggcgctc gaggctgccg agctgctcgg
      121 tggagaacgg cggccggcgc ccgcgcatcc ggaagagcgg cccgctcgcc ttgagcccga
      181 cgcgggcgag acctaccggc aggctgaggc gggggggctt cacgccgccg agggcgcaga
      241 cccgctcgac cagctcgcgc agggtgacga tctcccccgc cagcaggtag tcccggccca
      301 ccggcgcccg ctcgatcacc cgcagcatgc cgtcgaccac atcctccaga tagacccagc
      361 aggatttgcg gtccgccccc acgagggcgg gaaagcggcc gcggagcagc gcgcggagca
      421 gggcgttggc cccctccttc ttgcccggcg gaccgtagac caggctgggg tagacggtgt
      481 tgaccgcgag gccgcgccgc ccccactcac ggatcgcacg ctcgcccgag tgcttggtgg
      541 cgctgtagag ggtggggaac ggctggaggg gcgggctggt ctcggtggcg gcggagccgt
      601 cctccgggct ccccccgaaa taggcgatcg aggagacgga caggaaccgc ggcaccccca
      661 acttgtaggc cagggaggcc acgttctcgc tcccctggac gttggcctcg cgcatccgct
      721 ccggcggcgc gtcgggatcg agcaccgcgg cggcgtggat cacccagtcg gcgccggaca
      781 tcccctcgcg cagggagtag cggtcgccga tgtccccggt gaacgtcgtc accccgagct
      841 cgtggagagg ctccaccctg ctggtcgccc gcaccaccac ccggacctcg tgccccccct
      901 ccaccaggcg ctgagccagg gtcctgccga tgtagcccgt cgctccggtc acgaagatcc
      961 gcatggcgcg gattctagcg gagatcccgg ggagatcccg gaagcgccat ccaaaaaatc
     1021 ggatcggctc catccggaaa tttgaagatc ccggaagagc cgggaatctg gggctggggg
     1081 ctggtgttat ccaatgcgtg ggcgggtctc gggagttgag cccgccccgg agtccaaact
     1141 ccgacaactc cttcagctcc ctctcctccc cccgcaccgt ctccctccac ggtgcggggc
     1201 tttttattgg ctaaaggtcg aacgtcgcca ccaccggcgc gtggtcgctg gtaccgacgt
     1261 cggcgaggac gcgggaggcg gtggcgcgct cggccaccgg gcggctggcc aggatgtagt
     1321 cgatgcgcca gccgatgttg cgttgccgca tgttcctcca gggggcccac caggtgaaca
     1381 ggccgtcgtt gtcgggatcg aggtggcggc cgacgtccac caggtcctcg gcgaggatgg
     1441 cctcgaacag ctcccgctcc tcgggccgct ggccgatgag ccccggcttc ctctccttgg
     1501 gatggaggtc ctggtcggtc cgcgccacgt tcaggtcgcc gcagatcacc aggcgccgcc
     1561 cctcggcccg catggcggcg gcgtactcgc gcagcgactc caggaaccgc atcttggcgg
     1621 cgaagtcctt gccgccgttg ggcacgtaga ccgaggccac caccaggtcg ccaatctctg
     1681 ccgtgacgat gcgcgtctcg tggtcgaagg ccggatggga gtaccggggc ggctcgggag
     1741 agaggccgtc gcggacgttg aggctgaccc ccgaatagcc gccggcgccg tgccagtagc
     1801 acgagtaccc ctccaggccg cacagcgtct ccgagagctg ggagggggac gccttgatct
     1861 cctgcagaca gatgatgtcg ggccggtcgc ggccgaccca ctcgaggaac tgcgcctcgc
     1921 gggcgcggat gccgttgacg ttccaggtgg cgatggtcac agacatgcgc ggatcatagc
     1981 ccggttccct cccggcgtgc cgcgggtggc ggggaggggc gaatgatctt caccagatca
     2041 cggtgacttc acgcgcggcc gcaatgagag agtcgggggt caggatcggt atcccgtgga
     2101 tctgggccgt cgccatgatg atccggtcac agggatccga gtgaggagcg tccacctcgg
     2161 gagccaggcc ggcgacttca gaggtaacgg gcagctcggt cagcggatag gcactgagag
     2221 cccgcagaaa ccactgccgg ggaggcagcg gcagctggag tttccccttg cgatgcttga
     2281 tcgagatttc aaatgccgag atcgcggaga catagagggt gagatccggc tggagcaaga
     2341 gctcccgtgc cgggccggag agccgcgcgg gatctcctac ccaccagaga aggacacagg
     2401 tgtccaggag gaagcgcagc gcctactcct cgggccagtc ctcggcatca aggggtagag
     2461 aggggtcctc gtggaagatg atctttccca ggagagggtg aggttccaaa gaccggatgc
     2521 ctgcttccct cgcgcccgat cgtcgctcag cgaccgacgc aaggacgatc acctcggccg
     2581 ggccctgcgg gaagtcctcc ggcagatgga tctcgatctg atggtccggc gggacgttga
     2641 ccttgagctt gtgagcgtgc atcggcatca cctgtccgtc cctgaagcat acgcctccgc
     2701 cctcgagagc gccacacgca cagccattgc cgagggcggc cgggaccgct aagctcgcgg
     2761 cagcatgagc tctctccggt ccgccctccg cgcggcttcc gtggccctgg cggccgcctg
     2821 tctggcggcg cccgcctttt cggctcccgt tccccgccgg gaacgcgagg cgctgatcga
     2881 cttctatcgc gccaccggcg gcgacgcctg gggccgccgc gaccgctggc tgggcgctcc
     2941 gggaaccgag tgctcctggt atggcgtgtc ctgtgacgcc tccggctccg tggccagcct
     3001 ggtgctgaac accaacggcc tgaccggcgg cgtgcccgcg tcgatcgcgg gcctggaaaa
     3061 cgtccgcacc ctggtcctgc gggagaaccg gctggcctcg ctgccggcgg acctcggccg
     3121 gttgcagcgc ctggcctcgc tcgacgtcga ggccaacgcg ctccgcgggc tgccgccgga
     3181 gctgggccag ctccagggcc tgcgcttcct ccggctctcc cgcaaccccc tgggcgcgct
     3241 cccgggggag accctgggcg ccctctcctc gctcgagagt ctctacgccg acgggaccgg
     3301 tctctccgag ctgcccgccg agatcggccg cctcgagaag ctccgcttcc tgagcctccg
     3361 cgacaacggg ctgaccgccc tgccggcgga gaccgggagc ctccgggagc tgctcctcct
     3421 cgacctggtg aacaaccggc tggagactct gggacccgag atcggccgcc tcgtcgcgct
     3481 gaccaccctc gtcgccgacg gcaacctcct cgaggcgctg ccgccggaaa tctggcgcct
     3541 cccggcgctg acgagcctgt cgctgccggg caaccggctc tccagcctgc cgccgctgcc
     3601 gcctgaaacc acgccggagc ccgccctcct ctatctcgac ctctccggca accggttcac
     3661 caccttcccg gccgacgtgg gccgtctccg gtcgctgacc accctgcgtc tggcggacaa
     3721 ccagatcgcc gaggtctccg gcatcctcgg cgacctcccc gccatcgagc ggctggacct
     3781 ctcgcgcaac ctcctcaccg cggtgcctgc cggcttggtg cgtctcggca ccctggagag
     3841 cctgctgctc gcgggcaacc ggatctcagc cgtgccggcg gagctggcgg cgctcggcca
     3901 gctcttctcc ctggacctgt cggacaaccg gatcgcggcg ctgccgccgg agctgtcacg
     3961 gatggcggcg ctccgctggc tgcttctgcc ggacaacctg ctggcggacc tgcctgcgga
     4021 gctgggcggc ctcgggctcg agttcctcga cctggcgggc aatcacctcg agaccctgcc
     4081 ggcggcggtc ctcggcatgg cgagcctgga gatcctgaac ctgtcatcca accggatctg
     4141 gggggacatc ccgcgcgagt tggcggggct cgagcggctg gagctgctct acctctccgg
     4201 caacccgctg accccgggcc cgatccccga ctggctgcgg ggcctgccgt tgcgcggcct
     4261 cggcctgcgc gccaccaacc gcaccgggga gatccccgcc tggctcgggg acagccggct
     4321 cgagtcgctg gacctcggcc agaacccctt cgatccggcg ccggtccccg actccctgcg
     4381 ccggctcacc accctgcggt cgctctggct ggaccacacc ctccgcctgg gggccatccc
     4441 cgccatccag gaatggctcg gcgcgcggga cctcgtccac ctgtcgctgg cgagcaaccc
     4501 gttcaccccc ggcccgatcc cggacctgtc ggggagccgc aacctcttcc tcctcgacct
     4561 gggggagacg aaccggacgg gctccctgcc ggagtggctg gccggcgccc gctcgctggg
     4621 ctacctgctg ctcgagcgca accggctgga gggggggatc cccgagtcct ggggcgcgct
     4681 gaccagcctg cggacgctct ggctgcgctc gaaccgcctc accggccagg tccccgccgc
     4741 cctcaccgcc ctggggctgc tggaggagag ggccggcctc gacctgcgct ggaactccct
     4801 gcggcagagc gagccgggaa ccctggcctt cctgaacgcc aagcaggccg gccgggtgga
     4861 cttccgcgac acccagaccc tcaccccccg cggcgtggcg gcgcgctccc tgggaccgga
     4921 cagcgtgctc ctcacctggg atcccatccg ctaccgggcg gacgagggct actacgccgt
     4981 cttcaaggcg accgcgccgg aggggcccta caccctcgcc ggacggacgg ccagcaagca
     5041 cgacaccgcg ctggaggtca tcggcctcac cccccgcacc gtctactggt tcattgtgag
     5101 ggcggtcacc gagccccacg ccgagaacgc caaccgcgtc gtcagccccc cgacgccgcc
     5161 cgtccgggcg cggacgttcc cttagggctc tcgcacggag ccatctcccc tccccacgag
     5221 agcgccctcc cccgccggga gggcagctcc agcgtcagcc tgtgggaagc gtcctgcgtg
     5281 gcgacatgga tggcccggta cccgaccttg ggccggctcg cctccgcctg ctgaaggatc
     5341 tcgacgatgg ccgggatgaa aatcccttcg ggtgacaggc cgctgccctg ggcccggagc
     5401 tcccaggacg aaaagcagtc gtcccccgag cagctctcga cacagccgac ctggacgctg
     5461 tcgctgccgc tgaaccagtg gctccatctc gttccccgag ggtccgtgga ttgcccggcc
     5521 gggcgtccag ctaccgcgac cgcttcgccc aggctcatcc ctggccgcag cttgccgaag
     5581 accgacagct gggcgacctc ggacagctcc accccctcgt ccagccggat ccagatctcc
     5641 tgacgccacg ggccggggaa ccagccggtg tacgagctga tccgggccgt ggcgagcgga
     5701 ggggccacgt ccagctccgc ctcctcggat gagccacagg cggcgagggt caggagggcg
     5761 gcggacaggg tgaggcgacg gagaaggagg ggagccatgg tgtcagaact ctagcgcggt
     5821 tccagcgggt tcactccgcg gcacgctcgg cccactccgc gacactctat agactatgcc
     5881 agccatgaat ttccatgaag actgcatcgt catcgacgga cacaccgatg tccccatgcg
     5941 gttgtgggag gagccggcgg acctgtctca gcggctgac
//
LOCUS       DASPJI010000334         3412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_534604, whole genome shotgun sequence.
ACCESSION   DASPJI010000334 DASPJI010000000
VERSION     DASPJI010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534604"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2212)
                     /locus_tag="VEL74_08840"
     CDS             complement(<1..2212)
                     /locus_tag="VEL74_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYG62675.1"
                     /translation="MRPATVAGLLRFRAAERPERVAFAFLADGEVEAERLTYAELDRR
                     ARAIAARLRQILGPGERALLLYPPGLEFIAAFFGCLHAGVVAVPAYPPRARDRAQTRL
                     RAIACDAEPKAALTTSAILGGLEARSDELAEMEWIATDTLPVEGGLDLPEPSPDAVAF
                     LQYTSGSTATPKGVMVTHANLLHNERMIGEAFAQDEDSVVVGWLPLYHDMGLIGNVLQ
                     PVHAGATCVLMAPVAFLQRPARWLEAISRYRATTSGGPNFAYELCVRKVGPEERSRLD
                     LSSWRVAFNGAEPVREATLARFAEAFGPCGFRREAFYPCYGLAEATLFVTGGAPGKAP
                     RVLAVDPAALERHEVMAGRDEEARRLVSCGGPWEDQRVVVVEPETELECPPGRVGEIW
                     IAGPSVARGYWRNAEATGRDFGAVLTGPPSPPETLDGPFLRTGDLGFVADGELYVTGR
                     LKDLVIIRGRNHYPQDLELTAESSHPDLRAGSGAAFAVEVEGEERLVLVQEVERRLRG
                     DVSEVAEAIRQAVAAEHEVQVWEVVLVRPGGVPKTSSGKVRRSACRAAYLDGELPVVG
                     RSAVPSLPANLLAEGPFPLGGGGLGQGEGQDGGRLREGNLDGGHPHVGPPPAPTRPLP
                     GGGSPLPWLRAAAARLLGMAPEAVALDRPLTGLGLDSLSAVELKGQAEAALGVELPLA
                     DLLQGASLGELAERLEAGAPLEEMPPLRPLPREGDQPVSFGQRSLWFLERLAPEA"
     gene            complement(2464..>3412)
                     /locus_tag="VEL74_08845"
     CDS             complement(2464..>3412)
                     /locus_tag="VEL74_08845"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HYG62676.1"
                     /translation="RPEEVGTPVSIGRPVGNTRVLVLDRELRPVPIGVPGELCAGGPG
                     VARGYVGRPDLTAERFVPDPEGGFGERLYRTGDLGRFRANGLIEFLGRRDEQVKLRGF
                     RIELEEIEAVVARQPGVRAAVVTVREDAPGIRRLVGYVVPDREPAPTPQELRSSLQQS
                     LPEYMVPSAFVALQSLPLTGNGKVDRKALPPPPLPEGGGSGYTAPRNELERTIAAIWR
                     EVLGLQQVGVHDNLFELGGNSLVVIKLHGRLEQELERKLPIMELFRHTTIDALARALA
                     GDAPQREGGAEQARTRTQTRRESLAQLNEARARRRTRPE"
BASE COUNT          483 a         1367 c         1116 g          446 t
ORIGIN      
        1 cggcctccgg cgccagccgc tccaggaacc agagcgaccg ctggccgaag gagaccggct
       61 ggtccccttc ccgcggcagg ggccgcaacg gcggcatctc ctcgagaggg gcgccggcct
      121 ccagccgctc cgccagctct ccaaggctcg ccccctggag cagatcggcc agcggcagct
      181 ccacgcccag cgccgcctcg gcctgcccct tgagctccac cgccgacagc gagtccagcc
      241 ccagacccgt caggggacgg tccagcgcca ccgcctcggg cgccatcccg agcagccgcg
      301 ccgcagccgc ccgcagccag gggagggggc ttcctccccc tgggaggggt cgggttggag
      361 cagggggagg accgacgtgg gggtggcccc cgtcaaggtt cccttctctg aggcggcccc
      421 catcctgtcc ttccccctgt cccaacccgc cccctcccag gggaaaggga ccctccgcca
      481 gaaggttggc cgggagtgag gggaccgcgc tccgccctac caccggcagc tcaccgtcca
      541 ggtaggctgc tcgacacgcg ctccgccgca ccttgccgct ggaggtcttg gggactccgc
      601 ccggacgcac cagcaccacc tcccagacct gcacctcgtg ctccgccgcc accgcctgac
      661 ggatcgcctc ggccacttcc gagacatccc cccggaggcg gcgctccacc tcctgcacca
      721 ggaccagccg ctcctcccct tccacctcca ccgcgaacgc cgcccccgac ccggcgcgca
      781 ggtccggatg gctcgactcc gccgtcagct cgaggtcctg agggtagtga ttgcgcccgc
      841 ggatgatcac caggtccttg agccggccgg tgacgtacag ctcgccgtcc gccacgaatc
      901 ccaggtcgcc ggtgcgcagg aaggggccgt ccaatgtctc gggggggctc ggcggcccgg
      961 tcagcaccgc cccgaagtcc cgccctgtag cctcggcatt gcgccagtat ccgcgggcga
     1021 cgctcgggcc ggcgatccaa atctccccta cccggcccgg cgggcactcc agctccgtct
     1081 ccggctccac cacgaccacc cgctgatcct cccaggggcc gccacagctc accagccgcc
     1141 gcgcctcctc atccctccct gccatcacct cgtggcgctc cagcgcggcc gggtccaccg
     1201 ccagcactcg cggcgccttg cccggggctc ccccggtgac gaacaacgtc gcctccgcca
     1261 ggccgtagca gggatagaac gcttcgcgcc ggaagccgca cgggccgaac gcctcggcga
     1321 agcgcgccag cgtcgcctcc cgcaccggct ccgcgccgtt gaacgccacc cgccagctcg
     1381 acaggtccag ccgcgagcgc tcctccgggc cgaccttcct cacacacagc tcgtaggcga
     1441 agttcggacc gccactggtc gtcgcccggt agcggctgat cgcctccagc cagcgcgcgg
     1501 gcctctgcag gaacgccacc ggcgccatca gcacgcaggt ggccccggcg tggaccggct
     1561 gcaggacgtt cccgatcagc cccatgtcgt ggtacagcgg cagccagccc accacgacgg
     1621 agtcctcgtc ctgggcaaag gcctcgccga tcatccgctc gttgtgcagc aggttggcgt
     1681 gggtgaccat cacccccttc ggagtggcgg tcgagcccga ggtgtactgg aggaaggcca
     1741 ccgcgtccgg cgacggctcg ggcaggtcca gccccccctc caccggcagc gtgtcggtgg
     1801 cgatccactc catctccgcc agctcgtccg accgcgcctc cagcccaccg aggatggccg
     1861 aggtggtcag cgccgctttc ggctcggcgt cgcaggcgat ggcgcgcagc cgggtctggg
     1921 cacggtcccg cgcccgggga gggtaggcgg gcaccgccac cacgccggca tggaggcagc
     1981 cgaagaaggc ggcgatgaac tccaggccgg gggggtagag cagcagcgcc cgctccccgg
     2041 gtcccaggat ctgccgcagg cgcgccgcga tggcgcgggc ccgccggtcc agctcggcat
     2101 aggtgagccg ctcggcctcc acctcgccgt cggcgaggaa ggcgaaggcc acccgctccg
     2161 gccgctcggc ggcacggaaa cgcagcagcc cggccaccgt cgccggcctc acctcggacc
     2221 atactgcggg ctcggtcact ggctcattcc cccctccagg gaaagagaac gacagagagc
     2281 ctccgagccc ctcgccggga ggggaagaga ggtgcgcaga gagaggcgaa gcggatgtac
     2341 ttcattgatt tttcagggtt tgcgtctacg tcttcggccg cctaccagag ccggccctat
     2401 tatgcacggt gcaggggcgg gctccaggcg agccgccccc ctctgaggta aagaaagcgt
     2461 aaatcattcg ggccgggttc gtctacgggc ccgcgcttcg ttgagctgcg ccagcgactc
     2521 gcgccgtgtc tgcgtgcggg tgcgggcctg ctccgccccc ccctcccgtt gcggcgcgtc
     2581 cccggcgagc gcccgcgcca gggcgtcgat ggtggtgtgc cggaacagct ccatgatcgg
     2641 cagcttccgc tccagctcct gctccaggcg gccgtggagc ttgatgacga ccagcgagtt
     2701 gcccccgagc tcgaacaggt tgtcgtgcac cccgacctgt tgcaggccca gcacctcccg
     2761 ccagatcgcg gcgatggtcc gctccagctc gttgcgcggc gccgtgtagc cgctcccgcc
     2821 cccctccggc aacggtggcg gcggcaaggc cttgcggtcg accttgccgt tgccggtcaa
     2881 gggcaggctc tgcagtgcta caaaggccga cggcaccatg tactcgggga gcgactgctg
     2941 gagggaggag cggagctcct gcggcgtggg cgccggctca cggtccggca ccacataccc
     3001 gaccaggcgg cggatccccg gcgcgtcctc gcgcaccgtc accaccgccg cccggacccc
     3061 gggctgccgg gccaccaccg cctcgatctc ctcgagctcg atgcggaagc cgcggagctt
     3121 cacctgctcg tcgcggcggc cgaggaactc gatcaggccg ttggcgcgga aacgtcccag
     3181 gtcccccgtc cggtagagcc gctcgccaaa gcctccctcc gggtccggca cgaaccgctc
     3241 cgccgtcagg tccggccgtc ccacgtagcc gcgcgccacc ccgggaccgc cggcgcacaa
     3301 ctcgccgggg acgccgatgg gcaccggccg cagctcccgg tccagcacca gcacccgggt
     3361 attgcccacc ggccggccga tggagaccgg cgtccccacc tcctccggcc gg
//
LOCUS       DASPJI010000335         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_135203, whole genome shotgun sequence.
ACCESSION   DASPJI010000335 DASPJI010000000
VERSION     DASPJI010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135203"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2185
                     /locus_tag="VEL74_08850"
     CDS             <1..>2185
                     /locus_tag="VEL74_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG62677.1"
                     /translation="VAGELWIGGAGVARGYLGRPDLTAERFLPDPYAEDMDEPGSRLY
                     RTGDLARYLPGGRMEVLGRVDHQIKLRGFRIEPGEIEAALTALPEVREAAVVVREDSP
                     GDRRLVAYVAGDLEPGTAEELRRSLRERLPEHMVPAALVTLTALPLTPNGKVDRRALL
                     ATAAPEWRSPAGHRAPRTQVEEVLAGTWAELLGLERVGAGDHFFNLGGHSLLATQAMS
                     RLRSAFGIEMPLRELFEAPRLADFAARVEEALRAGAGRSTPPLVPIARPAAAGPFPLS
                     FAQQRLWLAAQLETDGALYNIPVALRVAGPLRAEVLGRCLAEIVRRHEVLRTVFTAPK
                     GSPAQWIQPSVQPSLPVVDLSALPEWEREPEAVALAREEASRPFDLTDLTERHRGPLL
                     RGLLLRLAADDHVVALTVHHIAGDGWSMGVLVREVTALYAAFAAGLPSPLPELPVQYV
                     DFAVWQRSWLEGEVLDGQLDYWRHRLAGLAPAELPADRPRPPARSGRGATLPVTLPRD
                     LAEALRALGRQEDATLFMVLLAGFSVLVHRHSANDRPVFGTDIANRRQAELEGLIGLF
                     VNQLVLSVDLAGDPTFRELLRRVRRDTLEAYTHQDAPFERLVELLRPDRDVSRTPLFQ
                     LKLVLQNAPFSAQSLPDLGVAPLEVPGQTAKFDLLLNLMETGAGIGGHAEYSTDLFEA
                     ATVARWLGGFALVLRTAAERPDAHLHEIEAVLARQEALEEERREQE"
BASE COUNT          258 a          817 c          809 g          301 t
ORIGIN      
        1 gtggccggcg agctgtggat cggcggcgcc ggcgtggcgc gcggctacct ggggcggccg
       61 gacctcaccg ccgagcgctt cctgccggac ccctacgcgg aggacatgga cgagccgggg
      121 agccgcctct accggacggg agacctggcc cggtatctcc cgggaggtcg gatggaggtc
      181 ctcggccgcg tcgatcacca gatcaagctg cgcggcttcc gcatcgagcc gggggagatc
      241 gaggccgccc tgacggcgtt gccggaggtg cgcgaggcgg ctgtggtggt acgggaggac
      301 tcgcccggcg accgccggct ggtcgcctac gtggccggcg acctggaacc ggggacggcc
      361 gaggagctgc gccgctcctt gcgcgagcgg ctgccggagc acatggtgcc ggccgcgctc
      421 gtgacgctca ccgcgctccc cctcacgccc aacggcaagg tggaccgccg ggcactcttg
      481 gcgacggcgg cgcccgaatg gcggagcccg gcgggccacc gggcgccgcg cacccaggtg
      541 gaagaggtcc tcgcggggac ctgggccgag ctgctcggtc tggagcgcgt cggcgcgggc
      601 gaccatttct tcaatctggg cggccactcg ctgctggcga cccaggcgat gtcgcgcctg
      661 cggagcgctt tcggcatcga gatgccgctg cgcgagcttt tcgaggcccc gcggctggcg
      721 gacttcgcgg cgcgggtgga ggaggcgctg cgggcgggcg ccggccggtc cacgccgcct
      781 ctggttccca tcgcccgccc ggcggcggcc ggaccgttcc ccctctcctt cgcgcagcag
      841 cggctctggc tcgcggccca gctcgagacg gacggcgcgc tctacaacat cccggtggcg
      901 ctgcgggtgg cgggcccgct ccgcgccgag gtgctggggc gctgcctggc ggagatcgtc
      961 cgccgtcacg aggtcctgcg caccgtcttc accgcgccca agggctcacc ggcgcagtgg
     1021 atccagccgt ccgtccagcc gagcctgccg gtggtcgacc tgtcggccct gccggaatgg
     1081 gagcgcgagc ccgaggccgt cgccctggcc cgcgaggagg cgtcccgccc gttcgacctc
     1141 actgacctca ccgagcgcca tcggggcccg ctgctccgcg gcctcctgtt gcggctggcg
     1201 gcggacgatc acgtcgtggc tctgacggtg caccacatcg ccggcgacgg ctggtccatg
     1261 ggcgttctgg tccgcgaggt cacggccctc tacgcggcgt tcgccgccgg tctcccctcg
     1321 cccctgccgg agctgccggt ccagtacgtc gacttcgccg tctggcagcg gagctggctg
     1381 gagggcgagg ttctggacgg ccagctcgac tactggcggc accggctcgc cggcctcgcc
     1441 ccggcggagc tcccggccga ccgcccgcgg ccgccggcgc gcagcggcag gggcgccacc
     1501 ctgcccgtga cgctgccgcg cgacctggcg gaggccctgc gcgcgctggg gcggcaggag
     1561 gacgcgacac tcttcatggt cctgctcgcc ggcttctcgg tcctcgtgca ccgccattcg
     1621 gcgaacgacc ggccggtctt cggcaccgac atcgccaacc gccggcaggc cgagctcgag
     1681 ggcctcatcg gcctgttcgt caaccagctc gtgctgagcg tcgacctcgc gggcgaccct
     1741 accttccgcg agctcctgcg gcgggtgcgc cgcgacaccc tggaagccta cacgcaccag
     1801 gacgccccct tcgagcggct ggtggagctg ctgcggccgg atcgcgacgt gagccgcacg
     1861 ccgctgttcc agctcaagct ggtgctccag aacgccccgt tctccgcgca gagcctgccc
     1921 gacctcggag tggctcccct cgaggtcccc ggacagaccg cgaagttcga cctgctgctc
     1981 aatctgatgg agacgggggc cggcatcggc ggccacgcgg agtacagcac cgacctgttc
     2041 gaagcggcga cggtcgcccg ttggctcggg ggcttcgctc tcgtcctgcg gaccgcggcc
     2101 gagcgcccgg acgcccacct gcacgagatc gaagcggtgc tcgcccgcca ggaggccctg
     2161 gaggaggagc ggcgggagca ggaga
//
LOCUS       DASPJI010000336         5198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_827655, whole genome shotgun sequence.
ACCESSION   DASPJI010000336 DASPJI010000000
VERSION     DASPJI010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827655"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..414
                     /locus_tag="VEL74_08855"
     CDS             181..414
                     /locus_tag="VEL74_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HYG62678.1"
                     /translation="MPIHDELKDELLQTDEEFRRLYDEHKDFERRLAEINQKSLLDQD
                     DEIEEKKIKLHKLALKDRMESILRNHREARVSA"
     gene            451..1089
                     /locus_tag="VEL74_08860"
     CDS             451..1089
                     /locus_tag="VEL74_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HYG62679.1"
                     /translation="MTFAKEGWPFVLPFAFLAALFFFLGRPGWGVAMLVLGFLVLLFF
                     RDPARSYEGEVGVVLAPADGLITRIDEIEDPELGPGRFHRIVTFLSVFDIHVQRVPAA
                     GEVVVSRYAVGRKVAAFREDAGDVNEKHLSVIRRPNGDLVGVRQIAGLLARRVVCYLK
                     EGDQVHRGQSMGLIKFSSRVDLLVPASYKLLVEKGQRLQNGKTPVAEPGDPR"
     gene            1086..1928
                     /gene="pssA"
                     /locus_tag="VEL74_08865"
     CDS             1086..1928
                     /gene="pssA"
                     /locus_tag="VEL74_08865"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HYG62680.1"
                     /translation="MTVKDAPRPRIRPVRRGAYMLPSLFTIGNILLGFYAVVLGLRAT
                     GAVPGALLPHDVFGTAALLVFGAAILDSLDGRIARMTGTESDFGKEYDSLADVFTFGA
                     VPALLTYLWGLSELGRAGWLVPFFFLVCTATRLARFNVQTKVVDARYFVGLPTPAAAG
                     TVCSILFFAPYAEWRSWMLVFTLIAMALIGALMVSTIRYHSFKKLDLRKRWSYRVFVI
                     IAAVILVIVFEPRATFLAIAVTYTLSGPAGYLWGRLRRRPGDADTMPPPLPPDAGGPG
                     PHLP"
     gene            1925..2869
                     /locus_tag="VEL74_08870"
     CDS             1925..2869
                     /locus_tag="VEL74_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HYG62681.1"
                     /translation="MSAIAIFHPTTLLGKELRETLGRQAKSWQDIRLLSTREDEVGTL
                     TEAAGAAALVKGFEPEELQGVATAFFCGPIAWNRPVLQALPADALALILSFDATIEDG
                     RPVVSGVNDEAAQPGSPLLSPHPAVVLLAHLLHPLVPLGLERTVATVVQPASMEGDAG
                     IEEVFEQTRQIVAMISRTPTPVFGAQLSFNLLPVPLPADPVSAQLDAVLPGAPPVSLQ
                     LVQGGIFHGISASVYVRLREGLDAKALRKALAGHPVLETADDPKHLGPIDSAASDKVL
                     VGSVRKSNDGGFWLWAVMDNLTRGGALNALEIAASATG"
     gene            3173..>5198
                     /locus_tag="VEL74_08875"
     CDS             3173..>5198
                     /locus_tag="VEL74_08875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG62682.1"
                     /translation="MSSIFQPLRSTVRGLLKTPGFTAVAIFTLAVGIGANSALFTVVN
                     SVLLEPLPYPQPERLVGVWSTAPGLGMDKFEQSDAAYILFRKETRTLEELALYRADTA
                     ALTGGDRTEQLPIARATASLFEVLQAQPVAGRALQEADERHGAEPVALLSHGLWQRRF
                     QGAESAIGSLLRLDGVAHRVVGVMPQGFHFPEAETELWIPVEIDAARASTGDFNYQAV
                     GRLRPGASTEAVAKELSALIPRLPEEFADSDLQPAMIEQAKFGTVVSPLRDDLVGEVE
                     RTLWLLLGSVGSILLIACANVANLFLVRAESRQREVAVRTALGATRGVIARVFLSESL
                     ALALAGGALGLLLAMAGTRVLVSLRPEGLPRLEEIGVDGSVVAFTVLLALLSGLLFGG
                     IAVLRYGAPALVPALKEGGRGGTAGRGRHRARISLVAAQVALALVLLVGSGLMVKSFW
                     RLHGVDPGVEPQGVLTAYMSIPQSEYPDGASVARFVQQLLERVQAVPGVVSAGTVTVL
                     PLNEGGSNSGHSIEDHPVPPGAVPHILPTRYVSPGYFETMGIPLLSGRTFDRIDPART
                     SDGVVISQALAERFWPGKSPLGRRLYPGLPDGDVKWFTIIGVVGSVREEGLHEKPAES
                     LYYSIRPLDLGRGEGGIPRSFRLVVRGRVDPATLAGPVREALWSIDRNVPL"
BASE COUNT          690 a         1934 c         1712 g          862 t
ORIGIN      
        1 ccacgcatga cgacactctc ttccatcctc acgaccacct ccgaccgggg gattggccgt
       61 gtccgggagg cggttctacg gtgacccagc tcgccgcagg ccgctccttg ctttttatcg
      121 ttcatcgcag gcgccggtcc acctcgtgga ccgcacttcc acagacaaag gaggcctttc
      181 atgccgattc atgacgagct gaaggatgag cttctccaga cggacgagga attccgccgc
      241 ttgtatgacg agcacaagga ctttgagcgg cggctggccg agatcaatca gaagtccctc
      301 ctcgaccagg acgacgaaat cgaagagaag aagatcaagc tccacaagct ggccctcaag
      361 gaccggatgg agagcatcct gcgcaaccac cgcgaggccc gggtctcggc ctgagccgcg
      421 accggacgcc acgattcgag cccgacgccc atgacgttcg ccaaagaggg ttggcccttc
      481 gtcctgccgt tcgcctttct ggcggcgctg ttctttttcc tgggccggcc cggttggggc
      541 gtggccatgc tcgtgctggg gttcctggtc ctcctcttct tccgtgaccc ggcccggagc
      601 tacgagggtg aggtcggcgt ggtgctcgcc cccgccgacg gcctgatcac ccggatcgac
      661 gagatcgagg accccgagct cggccccggc cgcttccatc gcatcgtgac gttcctctcc
      721 gtctttgaca tccacgtcca gcgcgtgccg gcggcgggcg aagtggtcgt ctcccgctac
      781 gccgtcggcc gcaaggtggc cgccttccgc gaggacgccg gcgacgtcaa cgagaagcac
      841 ctgagcgtga tccggcgtcc caacggcgac ctcgtcggcg tccgccagat cgcgggcctg
      901 ctggcgcgcc gcgtggtctg ctatctgaag gaaggggacc aggtgcaccg ggggcagtcc
      961 atggggctga tcaagttcag ctcgcgggtg gacctcctgg tgccggcgag ctataagctg
     1021 ctggtcgaga aggggcagcg gctccagaac ggcaagacgc cggtcgccga accgggggat
     1081 ccgcgatgac ggtgaaggac gccccgcggc cgcgcattcg cccggtccgg cgcggcgcct
     1141 acatgctgcc gagcctgttc accatcggca acatcctgct ggggttctac gccgtcgtcc
     1201 tggggctgcg cgcgacgggg gccgtaccgg gcgccctgct gccgcatgac gtcttcggca
     1261 cggcagcgct gctcgtcttc ggtgccgcca tcctcgactc gctggacggg cgcatcgcgc
     1321 ggatgaccgg cacggaaagc gatttcggca aggagtacga ctccctggcc gacgtcttca
     1381 ccttcggcgc ggtccccgcc ctgctcacct atctgtgggg gctctccgag ctgggccgcg
     1441 ccggctggct ggtcccgttc ttcttcctgg tctgcaccgc cacccgtctg gcccgcttca
     1501 acgtgcagac caaggtggtg gacgcccggt acttcgtcgg cctgcccacg ccggccgccg
     1561 ccgggacggt ctgctccatc ctcttcttcg ccccctacgc ggagtggcgg agctggatgc
     1621 tggtcttcac cctgatcgcc atggcgctga tcggcgccct gatggtctcc accatccgct
     1681 atcacagctt caagaagctg gacctgcgga agcgctggag ctaccgggtg ttcgtgatca
     1741 tcgccgcggt gatcctggtc atcgtcttcg agccgcgggc cacgttcctg gcgatcgcgg
     1801 tcacctacac cctctcgggg ccggcgggtt atctctgggg ccgcctgcgc cgccgtcccg
     1861 gcgacgcgga caccatgcct ccgccgctgc cgcccgatgc cgggggcccg ggaccccatc
     1921 tgccatgagc gctatcgcca tcttccatcc gacgacgctg ctcggcaagg agctgcgcga
     1981 gaccctcggc cggcaggcca agtcctggca ggacatccgc ctgctctcga cccgtgagga
     2041 cgaggtcggt accctgaccg aggccgccgg cgccgccgcc ctggtgaagg gcttcgagcc
     2101 ggaggagctc cagggggtcg ccaccgcctt cttctgcggc ccgatcgcct ggaaccgccc
     2161 ggttctccag gccttgccgg cggacgccct ggcgctgatc ctctccttcg atgccacgat
     2221 cgaggacggc cggccggtgg tctccggggt caacgacgaa gcggcccagc ctggatcgcc
     2281 gctgctctcg cctcacccgg cggtggtgct cctcgcccac ctgctccacc ctctggtccc
     2341 cctgggattg gaacggacgg tggccacggt ggtccagccg gcctcgatgg aaggggacgc
     2401 gggcatcgag gaggttttcg agcagacccg ccagatcgtt gccatgatct cgcgcacccc
     2461 gacgccggtc ttcggcgccc agctctcgtt caacttgctg ccggtacccc tgcccgccga
     2521 tcccgtgtcg gcccagctcg acgccgtgct ccccggagcg ccgccggtct ccctccaact
     2581 cgtccagggc gggatcttcc atggcatctc ggcgagcgtc tacgtccgct tgcgggaggg
     2641 cctcgacgcc aaggcgctgc gcaaggccct cgccggccat ccggtgctgg agacggccga
     2701 cgacccgaag cacctcggcc ccatcgactc cgccgccagc gacaaggtgc tggtgggctc
     2761 cgtccgcaag tccaacgacg gcggcttctg gctctgggcc gtgatggaca acctcacccg
     2821 cggcggagcg ctgaacgccc tcgagatcgc ggcctcggcg acaggctgac ggcgggctag
     2881 cctccgccgc tccccctctc cctcgtcccc ctgaggggat ccccttctat ctttcgttcc
     2941 ttttcttgtc cgcgcggcgt gccggccgcg ctcccgagtc gagacgaccc tggaagccgg
     3001 cagccgatat cccacctcct gccccaccac cctcgcccac ccccgagggc cccgaggcga
     3061 acgcttcggg gccctcttcc taggggctgg aaccttctcg ctccacgctc cgtaagggtc
     3121 ccagagggag acgacggtcc ttccccgttc cggctctctg gagggtgcca tcatgagttc
     3181 gatctttcag ccgctccgct ccaccgtgcg cggccttctc aagaccccgg ggttcaccgc
     3241 ggtcgcgatc ttcaccctgg cggtggggat cggcgccaac tcggccctgt tcaccgttgt
     3301 caactccgtg ctcctcgagc cgctccccta cccgcagccg gagcgcctcg tgggcgtctg
     3361 gtccaccgcg ccggggctcg gcatggacaa gttcgagcag tcggacgcgg cctacatcct
     3421 gttccgtaag gagacccgga cgctagagga gttggcgctc tatcgcgcgg acaccgcggc
     3481 gctgaccggc ggcgaccgaa ccgagcagct ccccatcgcc cgcgccaccg cctcgctgtt
     3541 cgaggtgctc caggcccagc cggtcgccgg gcgggcgctc caggaggcgg acgagaggca
     3601 cggcgccgag cccgtcgccc tgctctccca cggcctctgg cagcgccgct tccaaggggc
     3661 ggagtcggcc atcggctcgc tcctccggct cgacggcgtg gcacatcgcg tggtgggggt
     3721 catgccccag ggcttccact tcccggaggc cgagaccgag ctctggatcc cggtcgagat
     3781 cgacgccgcg cgcgccagca ccggcgactt caactaccaa gcggtgggac gcctgcgccc
     3841 cggcgcctcg acggaggccg tcgccaagga gctctccgcc ctgatcccgc gcctccccga
     3901 ggagttcgcc gacagcgacc tccagcccgc catgatcgag caggccaagt tcgggaccgt
     3961 cgtcagcccg ttgcgggacg acctcgtggg cgaggtcgag cggaccctct ggctgctcct
     4021 cgggagcgtc ggctccatcc tcctcatcgc ctgcgccaac gtcgccaacc tgttcctggt
     4081 gcgggccgag tcgcgccagc gcgaggtggc ggtgcgcacc gccctcggcg ccacgcgcgg
     4141 tgtgatcgcc cgggtgttcc tctccgagag cctcgccctc gccctggccg gcggagcgct
     4201 ggggctcctg ctggccatgg cggggacccg cgtcctggtg agcctgcggc ccgaagggct
     4261 cccccggctg gaggagatcg gcgttgacgg aagcgtggtc gccttcaccg tgctcctggc
     4321 cctcctctcg ggcctccttt tcgggggcat cgcggtgctc cgctacggcg cgccagccct
     4381 ggtgcctgcc ctgaaggagg gaggcagagg gggcactgcg ggccgcgggc ggcatcgcgc
     4441 gcggatctcc ctggtggccg cgcaggtggc actggccctg gtgctcctcg tgggatccgg
     4501 cctgatggta aagagcttct ggcggctgca cggcgtcgat cccggagtcg agccgcaggg
     4561 tgtcctcacc gcctacatgt cgatccccca atccgagtac ccggacggcg cctccgtcgc
     4621 ccgcttcgtc cagcagctcc tggagcgggt gcaggcggtc cccggcgtcg tctcggcggg
     4681 cacggtgacg gtgctccccc tgaacgaagg cggctcgaac agcggccaca gcatcgagga
     4741 ccatccggtg cccccgggcg ccgtgcccca catcctcccc acccgctacg tcagccccgg
     4801 ctacttcgaa accatgggca tccccctgct ctccggcagg acgttcgacc gtatcgatcc
     4861 ggcgcggacg tcggacggcg tggtcatcag ccaggccctg gcggagcggt tctggccggg
     4921 caagagcccc ctgggccggc gcctgtaccc cggtctcccc gacggagacg tgaagtggtt
     4981 caccatcatc ggcgtggtgg ggagcgtgcg cgaggagggg ctgcacgaga agccggccga
     5041 gagcctctac tactccatac ggcccctgga cctcggccgc ggcgaggggg gcatcccccg
     5101 ctccttccgg ctggtggtgc gcggccgggt ggaccccgcc accctggccg ggccggtgcg
     5161 cgaggccctc tggtccattg accgcaacgt gccgctgg
//
LOCUS       DASPJI010000337         5124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_79590, whole genome shotgun sequence.
ACCESSION   DASPJI010000337 DASPJI010000000
VERSION     DASPJI010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79590"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1072..1857)
                     /locus_tag="VEL74_08880"
     CDS             complement(1072..1857)
                     /locus_tag="VEL74_08880"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HYG62683.1"
                     /translation="MLTPPQHPFDLRGKVAVVTGASRGIGEAIATALAAAGADLVVAS
                     RKAENVQPIADLLNANGARAVAVACHTGRADQVAALAERAGEAFGGVDIVINNAATSP
                     HFGPLLAAEESHWDKTIEVNLKGYVHVIKACLPMLRERGGGSIVNVASVAGFVPHSGL
                     GVYGVTKAAVLMLTKTLAAELAPDNIRVNALAPGLIQTKFSEVLWSSPEAREKALATI
                     PQGRLGQPEDLTGAVLYLASDASRFTTGSVLIVDGGQTLEGVY"
     gene            1907..2062
                     /locus_tag="VEL74_08885"
     CDS             1907..2062
                     /locus_tag="VEL74_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62684.1"
                     /translation="MKGSRSAGMLLLGIYLILAGLTSLLGLSFNGMGLLMGILALLAG
                     ILLLIGR"
     gene            2100..2915
                     /locus_tag="VEL74_08890"
     CDS             2100..2915
                     /locus_tag="VEL74_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62685.1"
                     /translation="MRKTYFVKYYDKGSTVMAADQIAEALSARGYESRSIYPAEIPAV
                     RDSILVFIKTSKLPDLVRARRLGNKTVLDVQDTVVFKRRIKNRWLFDGLIFKNDRQVR
                     DFATPRHLSRVIYHQWDPRYEPNRVPPGEFRIAYLGLARSFSQWGQCPGLVCFDKDYF
                     RRALDFNCHFSVRETEREFLYKPNCKVSTAAGCDANLVTTRDITTLELLGPDYPFYAE
                     DTTESLTATIQRARESLGGPEWRRGLEIMKEVKERTSMDRIIDDYVRYFEDLG"
     gene            2927..3496
                     /locus_tag="VEL74_08895"
     CDS             2927..3496
                     /locus_tag="VEL74_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HYG62686.1"
                     /translation="MDLNLPYPLMTVPLLIFLARLCDVPLATLRIILLSRGLRRVAPL
                     VGFFEALVWLLAIGQVMQHLDRWTNYLAYAGGFAAGTWVGILVEGRLALGLLAVRIIT
                     QEDAPDLIERLRAKHFGVTDFAARGLSGNVRLIFTVIQRKDQGQVLEIIRAIHPKAFV
                     SVSDVRSVSEGFLPARSGGGLFGFLGKRR"
     gene            3619..4032
                     /locus_tag="VEL74_08900"
     CDS             3619..4032
                     /locus_tag="VEL74_08900"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011119515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HYG62687.1"
                     /translation="MAVEITGRYVGNLKVEMTHGPSGAVIRSAAPVDNQGDGSSFSPT
                     DLAAAALGSCMLTLIAIVGQRDGLDLTGLSFRLEKHMASNPRRIGSIPVVIEMPAGLT
                     PEQRKKLENAALTCPVHKSLSEEVEKPVRFVYEDG"
     gene            complement(4029..>5124)
                     /locus_tag="VEL74_08905"
     CDS             complement(4029..>5124)
                     /locus_tag="VEL74_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG62688.1"
                     /translation="LAMLGHELRNPLAPIRAATEVLRLSGAGDSSTRWASDVIHRQTR
                     QLSRLVDDLLDVSRVTRGKIELHREDIDLAAVVAQAVETSRPLIDSRRHTLTLALPAE
                     PVHLHADLTRLAQVVANLLNNAAKYMQEGGDIRLSAELQGDRVVLRVKDEGIGIPREM
                     LPRVFDLFTQVHGTLDRAESGLGIGLALVRSLVEQHGGTVEARSEGSGRGSEFVVTLP
                     VVEDRQLPLPGLAGAAAGRQSGGRRILVVDDNLDVAESMGLFLEMAGHEIRMAHDGEA
                     VLPLVESYRPEAVLLDIGLPGIDGYEVARRLRLDPRNDGVLLVAITGYGREQDRDRGK
                     MAGFDHYLVKPVDPDVLRALLASPRQMSVA"
BASE COUNT          745 a         1785 c         1793 g          801 t
ORIGIN      
        1 tgagcttcac cgagctcatc cgcaaggcct ctgagatcgc ccgtcacgtc ttggactctc
       61 cccgagcccg cagcaaacag gccccccaag ctgtccaggc tgcttttcag cttggagggt
      121 taagttagcg cctatgcccc ccggccccct ctcccatctc ccaccttccc ccgccgggag
      181 agggggagaa aaacatccgg ccgcaggggg cggtggtgcc tccccccctg ggcggtcggg
      241 cggtagggag tgggggaggt caggaggggg gttctgcaac gccgcgctcc tcccccaccc
      301 tcgtgcctgc gccgcttcgc ggctgaatgg cgtggctccg ccagaatcca tccctctgaa
      361 tcggatcgca tccggagcgc cgaggcagct tcccgggacg tccggactcc atccgattgg
      421 attggatacc atccggagcg ttcggcagct tcccgggacg tccggactcc atcccgttga
      481 tgtggatgag atccggagcg ctcgggaagt gtccgtcggg ctcgggatga ggtccgcggc
      541 gtccggatgg catcccgcct gctgtcgaag cttccaacgc gctgtcgaag cttccggccg
      601 gctagcgaag ctcccgacgc gctgtcagag tctccgccgg accgttgcaa gcttcgacgg
      661 gctgccggag tctccgacga gctgttggaa gcttccggcg ggttaccgga gcttccggcg
      721 cgccgtcaga gtttccaacg ggccgtcgga gcctccgacg agctgtccta ctctccggca
      781 cgccgccgaa cgctccgacg agttgtggaa gcttccggcg cgctgtcgga gtcttcgacg
      841 ggctgtcgga atctccggcg cgccgccgga agctccgcca agagaaaagc gcctcccccg
      901 cgacggcgga gaaggcgctg cgtccttttc tatagatagg cgggccgcgc gaggcggcgg
      961 ccgtcacggc gggggcgggg aggcaccacc gcccctacac caccacccct gttccggcgt
     1021 ccgtgatggt ccgtgacggt ccgtgttggt ccgtgttgtc cgtgtccggg cctagtacac
     1081 cccttccagc gtctggccgc cgtcgacgat cagcaccgag ccggtggtga agcgcgaggc
     1141 gtcgctcgcc aggtagagga cggcgccggt gaggtcctcg ggctggccca gcctcccttg
     1201 ggggatcgtc gccagcgcct tctcgcgcgc ctccggcgac gaccagagca cctcgctgaa
     1261 cttggtctgg atcaaccccg gcgcgagggc gttgacccgg atgttgtcgg gcgccagctc
     1321 ggcggccagc gtcttggtca gcatcaggac cgcggccttg gtcacgccgt agacgccgag
     1381 gccgctgtgg gggacgaacc cggccaccga ggcgacgttg acgatcgagc cgccgccgcg
     1441 ctcccgcagc atcggcaggc aggccttgat cacgtggacg tagcccttga ggttgacctc
     1501 gatcgtcttg tcccagtggc tctcctcggc ggcgaggagc ggcccgaagt gggggctggt
     1561 ggcggcgttg ttgatcacga tgtcgacgcc gccgaaggcc tccccggccc tctcggccag
     1621 ggcggccacc tggtcggcgc ggccggtgtg acaggcgacg gccaccgccc gggcgccgtt
     1681 ggcgttgagc aggtcggcga tcggctggac gttctcggcc ttgcggctgg ccaccacgag
     1741 gtcggcgccg gccgcagcca gcgccgtcgc gatcgcctcg ccgatccccc gcgaggcgcc
     1801 ggtgaccacc gccaccttgc cgcggaggtc gaacggatgc tgaggtggag ttagcatagg
     1861 gggccaacga tatcctacgc ggtacgaaga ggaggaggag gagacgatga aaggctctcg
     1921 aagtgccggc atgctcctgc tgggaatcta cctgatcctg gccgggttga cctcgctgct
     1981 cgggctgtcc ttcaacggca tgggcctgct catgggcatc ctcgccctgc tggcgggcat
     2041 cctgttgctg attggacgct gacgggacgc tgacacctga cgaccggctc cccatccgca
     2101 tgcgcaagac ctacttcgtc aagtactacg acaagggctc gacggtcatg gccgccgacc
     2161 agatcgccga agccctgtcg gcgcgcggct acgagagccg gtcgatctac ccggcggaga
     2221 tcccggcggt ccgcgactcc atcctggtgt tcatcaagac gtccaagctg ccggacctgg
     2281 tgcgcgcccg gcggctgggc aacaagacgg tgctggacgt ccaggacacg gtggtcttca
     2341 agcgccggat taagaaccgc tggctgttcg acggcctgat cttcaagaac gaccgccagg
     2401 tgcgggactt cgcgacgccg cgccacctgt cgcgggtcat ttaccaccag tgggacccgc
     2461 gctatgagcc gaaccgggtg ccgccggggg agttccgcat cgcctacctc gggctcgccc
     2521 gctccttctc gcagtggggg cagtgcccgg gcctcgtctg cttcgacaag gactacttcc
     2581 ggcgggcgct cgacttcaac tgccacttct cggtgcggga gaccgagcgc gagttcctct
     2641 acaagccgaa ctgcaaggtg tcgacggccg cggggtgcga cgccaacctg gtgaccaccc
     2701 gcgacatcac caccctggag ctgctcgggc cagactatcc gttctacgcc gaggacacga
     2761 cggagagcct gaccgccacc atccagcggg cgcgcgagag ccttgggggg cccgagtggc
     2821 ggcgcgggct ggagatcatg aaggaggtca aggagcggac ctccatggac cggatcatcg
     2881 acgactacgt ccgctacttc gaggacctgg gctgaagggc gagaccgtgg acctgaacct
     2941 cccctatccg ctgatgaccg tgccgctgct catcttcctg gcgcggctgt gcgacgtgcc
     3001 gctggcgacg ctgcgcatca tcctgctctc ccgcggcctg cggcgggtgg cgccgctggt
     3061 cggcttcttc gaggcgctgg tctggctgct ggcgatcggc caggtgatgc agcacctcga
     3121 ccgctggacc aactacctgg cctacgccgg ggggttcgcg gccggcacct gggtggggat
     3181 cctggtcgag ggccgcctgg ccctcgggct cctggcggtg cgcatcatca cccaggagga
     3241 cgcccccgac ctcatcgagc ggctgcgggc gaagcacttc ggcgtcaccg acttcgcggc
     3301 ccgggggctc tccggcaacg tgcggctgat cttcaccgtg attcagcgca aggaccaggg
     3361 gcaggtgctg gagatcattc gcgccatcca ccccaaggcc ttcgtctcgg tctccgacgt
     3421 gcgctcggtc agcgaggggt tcctgcccgc ccggagcggc ggcgggctgt tcgggttcct
     3481 ggggaagcgg cgctgataga ctcggccggc gagcccggtc tgcccggcgc aggcgactgg
     3541 caggtggtgg cagggagggg ggagcgcaca cgaaggagcc gagcagggca gaccgggcgt
     3601 gagactagga ggatggagat ggcggtcgag atcacgggca ggtacgtcgg caacctcaag
     3661 gtggagatga cgcacgggcc gtcgggggct gtgatccgca gcgccgcgcc ggtggacaac
     3721 cagggggacg gcagctcctt ctcccccacc gacctggccg ccgcggccct cggctcctgc
     3781 atgctgacgc tgatcgccat cgtcggccag cgcgacggcc tcgacctgac cggcctctcc
     3841 ttccggctgg agaagcacat ggcgtcgaac ccccgccgca tcggctcgat cccggtggtc
     3901 atcgagatgc ccgccggcct caccccggag cagagaaaga agctggagaa cgccgccctc
     3961 acctgcccgg tccacaagag cctctcggaa gaggtggaga agccggtgcg gttcgtgtac
     4021 gaggacggct aggcgacgct catctgccgt ggcgaggcga ggagggcgcg caggacgtcg
     4081 gggtccaccg gcttgaccag gtagtggtcg aagccggcca tcttcccccg gtcgcggtcc
     4141 tgctcccggc cgtagccggt gatcgccacc agcagcaccc cgtcgttgcg cggatcgagc
     4201 cgcaggcgcc gcgccacctc gtagccgtcg atcccgggca ggccgatgtc gagcaggacc
     4261 gcctccggcc ggtagctctc caccagcggc aggaccgcct cgccgtcgtg ggccatgcgg
     4321 atctcgtggc ccgccatctc caggaagagc cccatgctct cggccacgtc gagattgtcg
     4381 tccaccacca ggatgcggcg cccgccggac tggcggcccg ccgccgcgcc ggccagcccg
     4441 gggagcggga gctgccggtc ctccaccacc ggcagcgtca ccacgaactc gctcccccgg
     4501 ccgctcccct cgctgcgcgc ctcgaccgtc ccgccgtgct gctccacgag gctgcgcacc
     4561 agcgccagcc cgatgccgag gccgctctcg gcgcggtcga gggtgccgtg gacctgggtg
     4621 aagaggtcga aaacccgggg cagcatctcg cgcgggatgc cgatcccctc gtccttcacc
     4681 cgcaggacga cccggtcccc ctggagctcc gccgagagcc ggatgtcgcc cccctcctgc
     4741 atgtacttgg cggcgttgtt gagcaggttg gcgaccacct gggcgagacg ggtcaggtcg
     4801 gcatggaggt ggaccggctc ggccggcagc gccagggtca gggtgtgacg gcggctgtcg
     4861 atcagcggcc ggctcgtctc cacggcctgg gccaccaccg cggcgaggtc gatgtcctcc
     4921 cggtgcagct cgatcttgcc gcgggtcacc cgggagacgt cgagcaggtc gtccaccagg
     4981 cgggagagct gccgggtctg gcggtggatc acgtcgctcg cccagcgcgt gctggagtcg
     5041 cccgcgccgg agagccggag cacctcggtg gcggcacgga tcggcgccag cgggttgcgc
     5101 agctcgtggc cgagcatggc gagg
//
LOCUS       DASPJI010000338        10226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_534844, whole genome shotgun sequence.
ACCESSION   DASPJI010000338 DASPJI010000000
VERSION     DASPJI010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10226
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534844"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VEL74_08910"
     CDS             complement(<1..921)
                     /locus_tag="VEL74_08910"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HYG62689.1"
                     /translation="MARRRLLCLSLWTILTLPLPGLSQEGAPQEAPQARQLPAPRQLE
                     VRRAASPIQVDGVLDEPAWADALVFDIPFEWFPGDNTPGPVKTDFLVTYDDDSLYAAW
                     RAYDPDPASIRAHLADRDTLTTFAQDDHVVLMVDPFNDERRGFQFRINPLGVQADALF
                     SQNEQLEDFSYDMIWDSAARITDEGYVVEVAIPLDQIRFPRTPGPQTWGFNVDRSYPR
                     NVRHRMSASPIPRGLACVLCQAVKVTGFEDIEPGRNLEITPTLTAGRTDVIDRFPDGD
                     LEAGDEETDPGVSLRWGITPNITLSAAANPD"
     gene            1454..1526
                     /locus_tag="VEL74_08915"
     tRNA            1454..1526
                     /locus_tag="VEL74_08915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1487..1489,aa:Leu,seq:aag)
     gene            1639..2802
                     /gene="rnd"
                     /locus_tag="VEL74_08920"
     CDS             1639..2802
                     /gene="rnd"
                     /locus_tag="VEL74_08920"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HYG62690.1"
                     /translation="MPEQSHPPYVCIETDSELAAVARAWAQSPALGLDTEFVRERTFY
                     HRLGLVQVSDGTTAWLVDPLKIGDLGPFVEVLRSPGTVKVLHSASEDVEVFYRGLGAM
                     PAPLFDTQVAAGLAGIGPFLSYQKLVATLLGVELPKGETRTDWLARPLSEAQLAYAAE
                     DVVYLLPLYDHLRQELSRLGRLGWVEEDSAALLDPGRFEEDFDASYLRVKGSGRLNRR
                     QLAALQALSAWREREARRRDLPRNFVLREGFLLGLATRLPKTPQEVRRVPEFDANQAG
                     RDEATWLEILHHALELPDAELPPRVSHLPFSPGVKELTNRIRERAKKRAEALGVPPEL
                     LAPRRTLDALLRKALTGAADPLPRELDGWRREAVGEDLVQEVQAARAEGLLRG"
     gene            2894..3355
                     /locus_tag="VEL74_08925"
     CDS             2894..3355
                     /locus_tag="VEL74_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG62691.1"
                     /translation="MTIDEEVRRATKLLETVMQAAGLTRKDLDQKLGAGPGYVSQVLT
                     GRMELKYRHILAILRALDVDPGVFFQTLYPESKPSSDVAMMEEFLKRFQKLGFGRPPE
                     PVPPPPSARLDSGELEKLIQNAVRAALSDEGGPPRAGKGRGGKRTRTTEDD"
     gene            complement(3476..4786)
                     /locus_tag="VEL74_08930"
     CDS             complement(3476..4786)
                     /locus_tag="VEL74_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62692.1"
                     /translation="MLGELPSGETRRVLAHLLHGCDQCRAAMSPLAAAMFLPSHQEDE
                     VAVEAGAEYEFPIFRALASAKRVATLARGSADQALGAFPKEVPVLDALSSESTLPSVP
                     VLCERLLATCHALRHRDPEAMVLAATMATGLTERLEQSDVQGPKALADLRARAWAELG
                     NARRVANDPNGAEADFARALRQAASGTGDPLLLARLMDLAASLYIAQRRFNEAFRLLD
                     WVYQIYQREGDAHSAGRALIKKGISAGYALDSEQAVYLLGQGLGLIDAACDPKLVLSA
                     VHNLIWCLVDCSRLDEANRLLSQSRKLYRAHAERLDELKARWVEGRIAAGFGNEEEAE
                     RAFLEVRAGYEETDLSYDAALVSLDLAAIWLHEGRTAEIRSLIDEMLAIFRARNIHRE
                     AIGALLMLREAFEKDRATVTLLRNVASELQRLDREPTRRARPEP"
     gene            complement(4941..5258)
                     /locus_tag="VEL74_08935"
     CDS             complement(4941..5258)
                     /locus_tag="VEL74_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62693.1"
                     /translation="MSRRTVRRVTAAAVLALALLFAGPGTAAAGSLEVAVPAFQGSSL
                     LDRFLDWLDGLWDDQAAASDKSILTTGSPNSGTNAGNPTGSTSTVGGNGLGEQGGMID
                     PDG"
     gene            5300..5446
                     /locus_tag="VEL74_08940"
     CDS             5300..5446
                     /locus_tag="VEL74_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62694.1"
                     /translation="MAPADRGYPITGKPANLQGNFEKKDFREGATRFPDGPHALPAAE
                     TSIR"
     gene            complement(5751..6137)
                     /locus_tag="VEL74_08945"
     CDS             complement(5751..6137)
                     /locus_tag="VEL74_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62695.1"
                     /translation="MAAKAKTSYSIALVERYQLAKSLEANTSQIPHLESSRFRLQRML
                     EELRELMTEQDRLQARKQEVTRRIQDLEEQSGKLAAFLKVGIKEHYGTRSERLVEFGL
                     QPFRGRRRETPEAPLSDPEAPGRNTP"
     gene            complement(7217..7561)
                     /locus_tag="VEL74_08950"
     CDS             complement(7217..7561)
                     /locus_tag="VEL74_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62696.1"
                     /translation="MSNTRFHKLTLIVPEDRRLELTLPNDFPTGPAELIVLSAAAMSA
                     AAGAPPQPANRAEPRTSVLDELRSRLRTGRETHGWEELDAIRLRRKRGSARWNGSDRT
                     AGADKRRSPGRG"
     gene            complement(7662..8171)
                     /locus_tag="VEL74_08955"
     CDS             complement(7662..8171)
                     /locus_tag="VEL74_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62697.1"
                     /translation="MRDRRDSTVVPSKVFSPEYLRWLAETTNEPRSASEGDLAGPWRI
                     VPYEGNGYALLRQGERLRRDRRPPGLFSDLGTAQVAAAILPGVGRESLYRLRTEEKSL
                     GYAIEGEGDVVGWLDIFHQDVTAAMHVVACLLRSPGALARVLQAASGLTLEHTAEILL
                     ERAQHPEAP"
     gene            9148..>10226
                     /locus_tag="VEL74_08960"
     CDS             9148..>10226
                     /locus_tag="VEL74_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG62698.1"
                     /translation="MDTLASDLRQAARALARRPGFLAVAVLTLALGIGANTALFSVVH
                     AVLLRPLPYQDPERLVSIVHLIPLLDAELVGGADFIAWRDGARTLSAVTAYSATRFTL
                     TGGEQPERVRGARVSANFLSMLGLRPERGRDFRKEEERLNAGRVALVGRRLWDRLYGA
                     RGDVQEPGITLNGTSYTVIGVLPRDFQFPGESNIEVLLPFTLDETVESRRESMTLLNV
                     IGRLAPGVSIPQVHAELEVIRKRSEEEAARAAAARPPEPPPALPPGFEPPPGFPLPGG
                     APPAGGGGRQMRRMVVQGGPGAPGGAPAGPPGGGRVVAPGGPRGPGGPGSGAGRPPAF
                     LQDLVLEITPLRDKIVGDVRPALLVL"
BASE COUNT         1661 a         3486 c         3455 g         1624 t
ORIGIN      
        1 gtcggggttg gccgcggcgc tgagggtgat gttgggggtg atcccccagc gcagggagac
       61 gccggggtcg gtctcctcgt cccccgcctc gaggtcgccg tcggggaagc ggtcgatgac
      121 gtccgtgcgg ccggcggtga gcgtgggagt gatctcgagg ttgcggccgg gctcgatgtc
      181 ctcgaaaccg gtgaccttga cggcctggca gaggacgcag gcgaggccgc gcgggatcgg
      241 cgaggccgac atgcggtggc ggacgttgcg cggataggag cggtcgacgt tgaagcccca
      301 ggtctggggg ccgggggtgc gcgggaagcg gatctggtcg agggggatcg ccacctcgac
      361 cacgtagccc tcatcggtga tgcgggcggc cgagtcccag atcatgtcgt aggagaagtc
      421 ctcgagctgc tcgttctggc tgaacagggc gtctgcctgc acgccgagcg ggttgatccg
      481 gaactggaag ccgcggcgct cgtcgttgaa cgggtcgacc atgaggacca cgtggtcgtc
      541 ctgcgcgaac gtggtcaggg tgtcgcggtc cgccaggtgg gcgcggatgc tcgccggatc
      601 cgggtcgtag gcgcgccacg cggcgtacag gctgtcgtcg tcataggtga ccaggaagtc
      661 ggtcttgacc ggtcctggcg tgttgtcccc cgggaaccat tcgaacggaa tgtcgaagac
      721 cagagcgtcg gcccaggccg gctcgtcgag gacgccgtcg acctggatgg gcgaggcggc
      781 gcgccgcacc tcgagctggc ggggagccgg gagctggcgc gcctgcggcg cctcctgcgg
      841 ggccccctcc tgggacaggc cgggcagcgg cagggtcagg atcgtccaca acgacagaca
      901 caacaggcga cggcgggcca tacggggtct cctccgcatc ggaaaaatca gattgacgcc
      961 tccagggaag ccggggaagc ttacacgact cctacgcagc ccgcaccccg gagttgcgct
     1021 cgtggcggcg cccgtttcac cttgtcccgg agatggggcc gcggagcacc tagacgtcag
     1081 aagggtaaga agctagactg ttaacaagag aaagagaaag tcatggactg cgtgcgcctc
     1141 ctccccggtt tcaccggcat ccgagccttc caccggcctg cggacggacg aacctccgtc
     1201 cagcgagagg tcagtcggaa aacatcggat ctcggctgga atgaaccggc gaggcgcgcg
     1261 ggagctgtcg cgccttcctg acgccccgac tccagagcct gacattttca ggccttccgg
     1321 ggttggcgcc ccggaaggcc ttttcacttc cgcgcgcttc gcctccgatc catcccgctg
     1381 ccgggtccag aggaggaccg aggctcggcc ggaaccgtcc ggcgccgcgg atgcggcgcg
     1441 gagggtccgg ccccgcggct agctcagtca ggtagagcgc cgggcaaagg ccctggagag
     1501 ggcaggttcg aatcctgcgc cgcgcgacaa gaaccgggta tcgtctggac catcgcatgt
     1561 gccgccgccg cacccctccg gcgtctgctg gaggggacgg cggtttttct tcgcgcacct
     1621 tccccgtaca atcccccggt gccagagcag agccacccac cctacgtctg catcgaaacc
     1681 gactccgagc tcgccgccgt cgcccgcgcg tgggcgcagt ctcccgctct cggcctggac
     1741 accgaattcg tccgggagcg caccttctac caccgtctcg gcctcgtgca ggtctcggac
     1801 ggcaccaccg cgtggctggt cgatccgctg aagatcggcg acctcgggcc gttcgtcgag
     1861 gtcctccgct cgccgggtac ggtcaaggtc ctccactcgg ccagcgagga cgtggaggtc
     1921 ttctaccgcg gcctcggcgc catgcccgcg ccgctgttcg acacccaggt ggcggcgggc
     1981 ctcgccggca tcggcccctt cctgagctac cagaagctgg tcgcgaccct gctgggggtc
     2041 gagctgccca agggggagac gcgcaccgac tggctcgccc ggccgctatc ggaagcgcag
     2101 ctcgcctacg cggccgagga cgtggtctac ctcctccccc tctacgacca tctgcgtcag
     2161 gagctgtcgc ggctggggcg cctcggctgg gtagaggagg actcggcggc gctcctcgac
     2221 cccggccgct tcgaggagga cttcgacgcc tcctacctgc gggtcaaagg gtccggccgg
     2281 ctcaaccgcc ggcagctcgc ggccctgcag gcgctctccg cctggcggga gcgcgaggca
     2341 cggcggcgcg acctgccgcg caacttcgtg ctgcgcgagg ggttcctcct cggcctggcg
     2401 acgcgcctcc ccaagacccc gcaggaggtg aggcgggtgc cggagttcga cgccaaccag
     2461 gcagggcgcg acgaggcgac ctggctggag atcctccatc atgccctcga gctgcccgac
     2521 gccgagctgc cgccccgcgt ctcccacctc cccttctcgc ccggggtcaa ggagctgacg
     2581 aaccggatcc gcgagcgggc gaagaagcgg gccgaggcgc tcggggtccc gccggagctc
     2641 ctggcccccc gccgtaccct ggacgccctg ctgcgcaagg cgctcaccgg ggccgccgac
     2701 ccgctccccc gggagctcga cggctggcgc cgtgaggccg tgggcgagga cctcgtccag
     2761 gaggtccagg cggcccgggc cgaggggctg ttgaggggct agaggatcgc cggacgtggg
     2821 gggacggacc ggcggcacgt ctttccacca cgaaaatctt tggcgaagcc gccgagtgcg
     2881 atagactttg tgtatgacga tcgacgaaga ggtccggcga gcgactaagc tgctggagac
     2941 cgtcatgcag gccgcggggt tgacacgcaa agacttggac cagaagctcg gagccggtcc
     3001 aggctatgtc agccaggtgc tcacgggaag gatggaactt aagtatcgtc acatattggc
     3061 gatcctgaga gccttggacg tcgacccagg cgtctttttt cagaccctgt atccggaaag
     3121 caagccctcc agcgatgtgg cgatgatgga agaattcctg aagcgcttcc agaagctggg
     3181 attcggcaga ccaccggagc cggtgccgcc tccgcccagc gcgaggttgg attccggaga
     3241 gctcgagaag ctgatccaga acgctgtccg ggcggccctg tcggacgagg gtggcccacc
     3301 gagagcgggg aagggcaggg gcgggaagag gacgaggacc accgaggacg attgacctcg
     3361 gcggggcgtg actgcaggac caatttccag ctcagatcca atgagagagg gagggagagg
     3421 gggccgcggg gcggagaagg agtgtcttcg ccccgcggag gtgtacgcgc cctgatcagg
     3481 gctcggggcg agcccggcgc gtcggctcgc ggtcgagccg ctgcaactcg gacgccacat
     3541 tgcgcagcag cgtgaccgtg gcccggtcct tctcgaacgc ctcgcgcagc atcagcagcg
     3601 cgccgatcgc ctcgcgatgg atgttgcgcg cccggaagat cgccagcatc tcgtcgatga
     3661 ggctgcggat ctcggccgtc ctgccttcgt ggagccagat tgccgccagg tcgagggaga
     3721 ccagggcggc gtcgtacgac agatcggtct cttcatagcc tgcccggacc tccaggaagg
     3781 cacgctccgc ctcctcttcg ttgccgaatc cggccgcgat ccgcccttcg acccagcggg
     3841 ccttgagctc atccaaacgc tcggcgtgag cgcgatagag cttgcggctc tgagagagaa
     3901 gccgattcgc ctcatcgagg cggctgcagt cgacgagaca ccagatcagg ttgtgaaccg
     3961 ctgacaggac gagcttggga tcgcaggcgg catcgatcaa gcccagacct tggccgagca
     4021 gatacaccgc ctgctcgctg tccagcgcgt aaccggccga gatgcctttc ttgatcaggg
     4081 cgcggcccgc cgaatgagcg tctccttcgc gctgatagat ctggtagacc cagtcgagga
     4141 gccggaacgc ctcattgaaa cgccgctgtg cgatatagag ggaagcagcg agatccatca
     4201 acctcgccag gagcaaggga tcgccggtgc cgctagcggc ctgtcggagc gctctagcga
     4261 aatccgcttc cgcgccgttg ggatcgttcg cgacacggcg ggcattgccc aactctgccc
     4321 aagccctggc acgaaggtcc gcgagtgcct tcgggccttg aacgtccgac tgctcgaggc
     4381 gctcggtcaa gcccgtcgcc atcgtggccg caagcaccat cgcctctgga tcccgatgcc
     4441 gcagcgcgtg gcaggttgcc agcagccgct cgcagagcac tggcacggac ggaagggtgc
     4501 tctcggaaga gagcgcgtcc agtaccggaa cctccttcgg gaaagcaccg agcgcctggt
     4561 ccgcgctacc gcgggcaagg gtcgcaacac gcttcgccga agcgagagcc cggaagatcg
     4621 ggaactcgta ctccgctccg gcttcgacgg caacctcgtc ctcctggtgg ctcgggagga
     4681 acatcgcggc cgccaaggga gacatggcgg cgcggcattg gtcacagccg tgcagaagat
     4741 gagcgagcac ccgccgggtc tccccggatg gcaactctcc gagcatgaaa cgctccagct
     4801 cggtagagct agggtggtaa ccgcctctat ctcctcccga catcgaggcc tccaattcaa
     4861 atcatgcagt aagtcattgt ctcacgacgc ggacaactct gtcagcaccc acgcgcgagc
     4921 ggcgtcgagc aacccgtgct ctagccgtcg ggatcgatca tccccccctg ctcccccagg
     4981 ccattgccac caacggtaga cgtcgagccc gtgggatttc ccgcgttcgt ccccgaattc
     5041 gggctcccgg tcgtcaggat gctcttgtcg gaggccgcgg cctggtcgtc ccagaggccg
     5101 tcgagccagt ccaggaagcg gtccaacagg ctggagccct ggaaggcggg gaccgcgacc
     5161 tccagggacc cggcggccgc cgttcccggg cccgcgaaca ggagcgccag ggccagcacg
     5221 gcggcagcgg tcacacgtcg aacggttcta cgggacatcg cgctctcctt ttccccggaa
     5281 gagccggcac ggagccacca tggcccccgc cgaccggggc tatcccatca ccggcaaacc
     5341 ggcgaatctg caaggcaact tcgaaaaaaa ggacttccgg gagggcgcga cacgctttcc
     5401 ggacgggccg catgcactgc cggccgcaga gacgtccatc cggtaaccag ctccctccat
     5461 ccatgcaccc aaacgaagca aagtttccgc gcatcgtatc acacaggacg accgtccggc
     5521 gatatcaagc aattcctgca agactgacgc cggccgcgcc gagtacgtct ggcaagagaa
     5581 aagtttccgc gcgtcaactt aagaggtgca tccaggtgac caagacgtga caacgccgcc
     5641 ccaacctcgc atagacggaa ggttatgagc cgaacggtat gggcttacct gctgctccga
     5701 gctggaggac cgccagcgcc tggcctacaa cctggagctg ctcgaccggc tcaaggagtg
     5761 tttcgtcccg gtgcctcggg atcgctgagg ggagcctccg gcgtctcgcg ccgcctgccg
     5821 cggaacggct gcaggccgaa ctccaccagc ctctcactcc gggtgccgta gtgctccttg
     5881 atgccgacct tgaggaaggc ggccaacttc ccgctctgct cctcgagatc ctggatgcgc
     5941 cgggtgacct cctgtttacg ggcctggagc cggtcctgct ccgtcatgag ctcccggagc
     6001 tcctccaaca tccgctgcag acggaagcgc gaagattcca ggtggggtat ctgggaggtg
     6061 ttcgcctcca gcgacttggc gagctggtat cgctccacca acgcaatgga atagctggtc
     6121 ttggcttttg ccgccatgtc ttgatctcct ctgagtgacc gcctccgcta gtccggagcc
     6181 gttctgattt gttgtcgtcg attgtacacc aggagaggag atgcaaggaa caaggtctag
     6241 ccttctcgag gaggaggcct gcctttcggg agaggtgtga tgccttccca aggaggcgcg
     6301 tgacctctcg tgtgaggcgc gcggcctccc cggacaggag gtccgccttc ttgaagaggc
     6361 agagctcctc gtcgggaagg cgtccggcct tcacgggaag gcacctctcc tccccttgga
     6421 gtcgaagccc ctcctccgga aggccgagcg cttgctccgg caggcgggtc tccttccgga
     6481 gtaggaacag ctcctactga aagaggcgcg gctccttccc agggaggcgg accgcctgcc
     6541 tcggaaggag ccccatcttc ccgggtagac ggctcgcctc ctccgcaagg tgtcgcgcct
     6601 gctgcgggag gcggcgcgcc tcctcgcaaa ggcgaggcgc cttcccgggg aggagaagcg
     6661 ccttctcatg actccatgga ggcttggcgg cacggatccg ccgctacccg cgctcgaagc
     6721 gcaggccggg gtcgtgccgg gccttctctc cgctccggcc gtgcggtcag agccgttcca
     6781 gcgggcgctg cctctcttgc ggcggagacg gatggcgtcc agctcttccc agccgtgggt
     6841 ctcgcggccg gtgcggagac gggagcgcag ctcgtccagg acggaggtcc taggctcggc
     6901 gcggttcgcg ggctggggcg gcggctaatc tttgagccgg ggggcggtgc gaagagctgg
     6961 gagcgtccgc tccctttacc ttcaccgaag ccgaagaaac tcctcaacgg agaagcccgc
     7021 atccttcagg atgcggattg cgagaccacg tccgagatcc acattgtcgt gcaatggaat
     7081 ggtgaccgcg actctcttgg cttcattggc taccgctttg gcgatcttct acaaaccctt
     7141 gagacttcaa gaagcgcagg agctcccgcg ccagtcctct ctcgtcgtag tggcctattc
     7201 agcccgtcgt cccccttcac ccccgcccgg ggctcctccg cttatccgct ccggccgtgc
     7261 ggtcggagcc gttccagcgg gcgctgcctc tcttgcggcg gagacggatg gcgtccagct
     7321 cttcccagcc gtgggtctcg cggccggtgc ggagacggga acgcagctcg tccaggacgg
     7381 aggtcctcgg ctcggcgcgg ttcgcgggct ggggcggcgc ccctgccgcg gcggacatgg
     7441 cggcggccga gaggacgatc agctcggcgg ggccggtggg aaagtcgttc ggcagggtca
     7501 gctccaggcg ccggtcctcc gggacgatca gcgtcagctt gtggaatctc gtgttcgaca
     7561 tctagatacc tctctcctga attcaatggg aggagaaggg ggagcggcct ccggagaccg
     7621 cccccttctc tatgatgacg agtcgcctac ccccgggtgg gtcagggcgc ctcgggatgc
     7681 tgcgcccgct cgagcaggat ctcggcggtg tgctccaagg tgagaccgct ggccgcctgg
     7741 agaacccggg ccagcgcgcc cggcgagcgc aagagacagg ccacgacatg catcgccgcg
     7801 gtcacgtcct gatggaagat gtccagccaa ccgacgacgt ccccttcccc ttcgatggca
     7861 tagcccaggg acttctcctc cgtccgcaga cggtacagag actcccggcc cacacccggg
     7921 aggatcgccg ccgcgacctg cgccgtcccc agatccgaaa agagacccgg cggacggcgg
     7981 tcccggcgta accgctcccc ctgacgcagc agcgcgtacc cattcccctc gtacggcacg
     8041 atccgccacg gaccggccag gtcaccctcc gacgcgctcc gcggctcgtt cgtggtctcg
     8101 gccagccaac gcaggtactc cggggaaaac accttcgatg gaacgacggt agaatcacga
     8161 cgatctcgca tggtttgctc cttgcggggt cgggcccggt ttgaggcctc aagactcatt
     8221 ccgggccgct ggttttgtct ctccggccgg ggacaccccg gccgcttcgc ttcagctctt
     8281 cagatttccg gtacggccaa tcttacacct cctcgcatga cgcttcacac gtctccaggc
     8341 cggccctcac ctcccctcgg aaaacttatc tccgactgac ggatgagagt ctatgacgaa
     8401 tcctgagcgt aagtcaacag ggattttgga gagaatgagg ggaaagttgg tcctcacccc
     8461 tcgccagagg tgaggaccca tgccggtcag cagaggaacc cggtctcgcc ggggcaccag
     8521 gtagcgacgc cgtcgcacat ggcgaagcag tagcccggtc ctccgctgca gctgtacgga
     8581 tccgaccccc agcaccagac ggagccacca cttggacaag gcgtttcaac gaagcaatct
     8641 ggcggcggcg ggcaggggga cgggcaatag accttcgagc cgccggtgca ttgggcgtag
     8701 cccctctggc cggcggagca gttgcggtcc gtcgccgtgc aggttccccc gcagttcacg
     8761 tagaccgacg ttccgccgcc acaatcggcc gagcaatacc ccttttcacc tgtgactccg
     8821 gacggtttgg ctggccggcg cgccgacggc tggtccgccg cctccctttg gagctgggcg
     8881 aagaactccg cgcgagacgc gccatcgccc cgggcctcca ccgcgacaaa gccgagacac
     8941 agagccaccg acagaatcca gccgagccta cggtctttca tgctgcctcc tttcaaaatc
     9001 ctgcgtctac acttaaaggt gcatgaacga ctgcgccgcg agtcgacctg cccgcttccc
     9061 cctggaacct ttgccctcgc cgtgcgttct aagagacaaa aagcccgcgc ggctctcccg
     9121 ccggtgccat gggaaggggt ctcctggatg gatacgctcg cctccgatct ccggcaggcc
     9181 gcgcgcgccc tggcgcgccg gccggggttc ctcgccgtcg cggttctcac cctggcgctg
     9241 gggatcggcg ccaatacggc cctgttcagc gtcgtccatg cggtgctgct ccgccccctc
     9301 ccctaccagg atccggagcg gctggtgtcg atcgtccacc tcatcccgtt gctggatgcc
     9361 gagctggtgg ggggcgccga cttcatcgcc tggcgggacg gcgccaggac gctgtcggcg
     9421 gtcacggcct actccgccac ccgcttcacc ctcaccggcg gcgagcagcc cgagcgcgtg
     9481 cggggcgccc gggtctccgc caacttcctg tcgatgctgg ggctccggcc ggagcgcggg
     9541 cgcgatttca ggaaggagga ggagcggctg aacgccggcc gcgtcgccct cgtcggccgc
     9601 cgcctgtggg accgcctgta cggcgcgcgg ggggacgtcc aggagccggg catcaccctg
     9661 aacggcacca gctacacggt gatcggcgtc ctcccccgcg atttccagtt ccccggggag
     9721 tccaacatcg aagtgctcct ccccttcacc ctggacgaga cggtggagag ccgccgggag
     9781 agcatgacgc tgctcaatgt catcggccgg ctcgccccgg gggtcagcat cccccaggtg
     9841 cacgcggagc tcgaggtgat ccggaagcgg tccgaggagg aggcagcccg cgccgccgcg
     9901 gcgcgtcccc cggagccgcc gcccgccctg cccccgggat tcgagcctcc ccccggtttc
     9961 cctctcccgg gaggcgctcc gcccgcggga gggggcggca ggcagatgcg gaggatggtg
    10021 gtccagggtg gaccgggcgc gccgggcggg gcgccggcag gcccgcccgg cgggggccgg
    10081 gtcgtcgctc ccggcgggcc gcggggaccc ggagggccgg ggagcggggc cgggaggccg
    10141 ccggccttcc tgcaggatct cgtcctcgag atcaccccgc tgcgggacaa gatcgtgggc
    10201 gacgtccggc cggcgctgct ggtgct
//
LOCUS       DASPJI010000339         9170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_364507, whole genome shotgun sequence.
ACCESSION   DASPJI010000339 DASPJI010000000
VERSION     DASPJI010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9170)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9170
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364507"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..6497
                     /locus_tag="VEL74_08965"
     CDS             <1..6497
                     /locus_tag="VEL74_08965"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62699.1"
                     /translation="LTEIGHAAAGELPRRPFAHEVIAARAAERPAAVAVSHGGEVVTY
                     GELDRRAEGIAHRLRAWGIGCEDRVGIFGERGVGMLATILGTLKAGTVFVPLDPRHPD
                     PRLRAVLETAELRLIATQNELAARARELGPSVLCWDEPDTSDAADAAAVVEIDPRQLA
                     CVFYTSGSTGIPKGAMVEHAGMLNHLWAKIDLLGLDAASVVVQNASHCFDISVWQFLA
                     ALMTGGRVMIYGEETAIDPAGLLRAIRRDRVTVLETVPTLLEPMLQAAEEFAAAGESV
                     DLPDLTRLISNAETLPVPLCRRWFERYPSVPLLNTYGATECSDDTTHQVMHTPPPPDT
                     LRVGVGRPIPGFRIHVLDPHLDLAPFGCPGQIGMAGVGVGRGYLGDPVKTAATFVPDP
                     FAEEPGDRLYLTGDLGRWTPDGELDFLGRRDHQVKIRGHRVELGEVEAVLARYHGLRE
                     VVVLALEAPAGGQQLVAYLVPGAAGLDVAAVRAFLRERLPEPMVPTAFVELAALPLTA
                     NGKVDRRALPAPEPQEREAGEHPRTAVQVLLATVWAEVLGRERVGLADDFFALGGHSL
                     LATQVTSRVRAVFGVELPVRAVFERPTVVEMAELVAAALGSGSSPHTPPLVPVPRDRP
                     LSLSFAQQRLWFLDQLEPGSPLYNIPAAVRLDGPLDPRLFGRVLGEIVRRHEVLRTTF
                     LTVDGRPVQAISPPQPFPLPIVNLTGLGGAERQTQAARLAAAESVRPFDLERGPVLRA
                     ALLRLQERQHVLLVTLHHIAGDGWSVDLFLRELALLYHAFAEGRPSPLPELAIQYADF
                     AVWQRSWLAGEVLESQIAYWRERLAGAPAVLDLTTDRPRPAVPGHRGERWPVEPPAAL
                     AEELRRLGRREGATLFMVVLAAFQTLLARHSGQGDVVIGTPIAGRDRLETEELIGFFV
                     NTLALRIDSAGDPSFRELLARVREVTLGAYTHQELPFERLVETLQPERHLNRSPLFQV
                     MFAFQNVPQGGPGIPGLAISALPVDLEVAKFDLVLTLGESAAGLTGTLEYDADLFDRS
                     TAARLASHLNRFLDEIAADPARRVSEVPQLAPAEQHQLLVEWNDSAADYAGAVCLHEL
                     IESQVERSPDAVALSCGGEELSYAELERRSNLLAHRLRELGAGPEVPVAMCVERSLAM
                     LVGLLAVLKAGGAYVPLDPDYPSERLASMIEDSGASLLLAQSHLVPRLPAGNARVLLL
                     DHEDGAAAPGTGRPPARPIGGAMPDSLVYVLFTSGSTGRPKGAAISHRAILNRLLWMQ
                     EALRLSAKDRVLQKTPFGFDVSVWELFWPFVTGACLVVARPGGHRDNAYLAHLIAEEE
                     ITVLHFVPSMLQLFLEESSDRGGAGGCGTLRDVVCSGEALPSELVRRFAARLGHARLH
                     NLYGPTEAAVDVTSWVCDEAGNDHGGIPIGRPIANTRIHLLDRNLLPVPLGVAGELFI
                     AGVNLARGYIGRPDLTADRFLPDPEAKDPGERVYRTGDLARWRGDGAIEYLGRLDHQV
                     KIRGVRIELGEIEAALTELPGVREAVVMARSEKGDGDKRLVAYVVGDVPAGELRQALW
                     ERLPEAMVPSAFVKLAALPLNANGKVDRKALSAGAVPEQPGAGEEGYVAPRTREEEIL
                     AGVWTQILRLPRVGVNDNFFELGGDSILSVQIVARARQAGLLFTTRQVFEHQTVAGLA
                     RHAEVADAAAVSRAEQGPVTGEVPLAPMQQWFLGDDPVDAHHFNQSLLLQSGQALDPV
                     ALEQVIGYLLAHHDALRLRFVREREGWRQRNDPPGRVPFHRVDLTGLPAARRAPSLEA
                     AAAQAQACLNLSDGPLLRAVFFTFGGEKGERLLLAVHHLGMDGVSWRILLTDLETGYT
                     QITRGQALRLPQKTTSFKGWAERLAGYARSQDLRSELGYWLAGPRMQAARLPLDGSGD
                     EDGWSPATLSISLSTEETRCLVQEVPAASRAHVQELLLAALALALRRWTGKDALLIDM
                     EGHGREEVFGDVDLTRTVGWFTTLFPLLVDLQGEREPGAVLARVQGQVRAVPGRGFGY
                     GVLRYLSPDPEVAERLAVLPPAQIVFNYLGRFDGVAETSSLFHLAPESTGPTSSPRLK
                     RKHLLEVGAWVGGGRLVVTFTYLETAHRVATIEALTGDFLASLRTLIALCLAGGPPEV
                     LPPDLALGIDPKELEEALHEIEFEGWETNEQA"
     gene            6484..>9170
                     /locus_tag="VEL74_08970"
     CDS             6484..>9170
                     /locus_tag="VEL74_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62700.1"
                     /translation="MSKRNVESAYTLSPLQEGMLFHSLYEPEAGVYVTQLGCVLDGQL
                     DLEAFTQAWRRLMERHSILRTAFAWKNTGRPLQVVGREVRLPLVTEDWSDLPSDERER
                     RWQDYLAADRKRGLDPAKAPLMRLALVREAPDLHRFLWTHHHILLDGWSIAKVFEELF
                     VLYEAHRQKRDVDLPLSPPFRDYIVWLGRQDLTQAEAFWRAELAGFSAPTPLWPGASA
                     PSPTKEGAAAEGQDLQIRQLPGSTVDALRAWARSCQLTPNTLVQAAWALMLARYSGEE
                     DVVFGITVSGRSPSLPGVEAMVGLFINTLPLRVDVRRGQELLPWLRRLQERQFGLRQY
                     EFSPLVEIQGWSEVPRGLPLFESFLVFDNYPVERRVGENGNGLEVRDVQAVEHVSYPL
                     SLTSSSDLRLQVSYDAKRFEATAVSRLLGHLEMLLESFVARPEGRLEDMVLLTGPERR
                     QLLVEWNSAGEPVAAGECLHELFEAQAARIPDAVALAWEGERLTYRDLDVRANRLARH
                     LRRLGVGPEIPVGVCLRRSPDLMVSLLAILKAGGAYVPLDPEYPAQRLAAMVPALVIV
                     ARDTAECLPVSQGIRLVRLEDAALAVAAEPGVAPAPLAVSGNLAYLLYTSGSTGAPKG
                     VAIEHHSVVALIDWARRAFSSDDLAGVLASTSINFDLSVFELFLPLSTGGTVVLARDA
                     LHLAELAEAGQVSLVNTVPSAMAELVRLAAVPASVRTVNLAGEPLRAELVREIYRQPT
                     VDRVLNLYGPSEDTTYSTWAEVPRGFEGAPSIGRSVAGSRAYLLDRWREPVPPGVQGE
                     LYLGGPGLARGYFRQPAWTAERFGPDPLADTEPGSRLYRTGDLARFRPDGQIEFLGRT
                     DHQVKVRGFRIELHEIEVALAAHPQIREAVVVVREDTPG"
BASE COUNT         1229 a         3142 c         3326 g         1473 t
ORIGIN      
        1 tgctcaccga gatcggccac gccgcggcgg gggagctgcc gcggcggccc ttcgcccatg
       61 aggtcatcgc cgcacgggcg gccgagcgcc ccgcggccgt ggccgtgagc catggcggcg
      121 aggtggtgac ctacggcgag ctggaccggc gcgccgaagg aatcgcccac cggctgcgcg
      181 cctggggtat cggctgcgag gaccgggtgg ggatcttcgg cgagcgcggc gtcggcatgc
      241 tggcgacgat cctggggact ctcaaggcgg ggacggtctt cgtgccgctc gacccccgcc
      301 accccgaccc gcgtctgcga gcggtcctgg agacggcgga gctgcgcctg atcgccacgc
      361 aaaacgagct ggcggcgcgg gcccgggagc tggggccgag tgtgctctgc tgggacgagc
      421 ccgatacctc cgatgccgcc gatgccgccg ccgtcgtcga gatcgacccc cgccagctcg
      481 cctgtgtctt ctacacctcc ggctcgaccg ggatacccaa aggggcgatg gtggagcacg
      541 cggggatgct caaccaccta tgggcgaaga tcgacctgct gggtctggac gccgccagcg
      601 tcgtggtgca gaacgcctcc cactgtttcg acatctcggt ctggcagttc ctcgccgccc
      661 tgatgaccgg cgggcgcgtg atgatctacg gggaggagac ggcgatcgac ccggccggcc
      721 tgctgcgcgc catccgccgc gaccgcgtca ccgtgctgga gaccgtaccg acgctgctcg
      781 aaccgatgct gcaggcggcc gaggagttcg cggcggcggg ggagagcgtc gacctgccgg
      841 acctgacccg gctcatctcc aacgccgaga cgctgcccgt ccccctctgc cgccgctggt
      901 tcgagcgcta cccgtccgtg ccgctgctca acacctacgg cgcgacggag tgctcggacg
      961 atacgaccca tcaggtgatg cacaccccgc cgccgccgga cacgctgcgg gtcggggtgg
     1021 gaaggcccat cccggggttc cgcatccatg tgctggaccc gcacctcgac ctggcaccct
     1081 tcggctgtcc gggccagatc ggcatggccg gggtcggcgt gggccgtggc tacctgggcg
     1141 acccggtgaa gacggcggcg accttcgtgc ccgacccgtt cgcggaggag cctggcgacc
     1201 ggctctatct gaccggggac ctggggcgct ggacgccgga cggggagctc gacttcctgg
     1261 gccgccggga tcatcaggtg aagatccgcg ggcaccgcgt cgagctcggt gaggtcgagg
     1321 cggtgctggc tcggtaccac gggctgcggg aggtggtggt gctggccctc gaggcacccg
     1381 ccggcggcca gcaactggtc gcctacctcg tgccgggtgc ggcggggctc gacgtcgctg
     1441 cggtacgtgc cttcctgcgg gagcggctgc cggagcccat ggtgcccacc gcattcgtcg
     1501 agctcgccgc gctgccgctc accgccaacg gcaaggtgga ccgccgggcg ctgcccgcgc
     1561 cggagccgca ggagagagag gcgggagagc acccgcggac cgcggtccag gtgctgctcg
     1621 ccaccgtctg ggccgaagtc ctcggtcgcg agcgcgtcgg cctcgcggac gacttcttcg
     1681 cgctgggtgg gcactcgctg ctggccactc aggtgacctc gcgggtccgc gcggtcttcg
     1741 gggtcgagct gccggtgcgc gcggtcttcg agcggccaac ggtggtggag atggccgagc
     1801 tggtggcggc agcgctcggc tccgggtcct cgccgcacac cccgcctctg gtgccggtgc
     1861 cgcgcgaccg cccgctgtcg ctgtcgttcg cccagcagcg cctctggttc ctcgaccagc
     1921 tagagcccgg gagcccgctg tacaacatac ccgccgccgt gcgcctcgac ggaccgctcg
     1981 atccgcgcct cttcggcagg gtgctcggcg agatcgtccg ccggcacgag gtgctgcgca
     2041 ccaccttcct cacagtggat ggacgcccgg tccaggcgat ctcgccgccg cagccgttcc
     2101 cccttcccat cgtgaacctt accggcctgg gaggcgcgga gcggcagacc caggcggcgc
     2161 ggctggcggc ggcggagtcg gtgcggccgt tcgacctgga gcgcgggccg gtgctgcggg
     2221 cggccctgct gcggctccag gaacggcagc acgtgctgct cgtcactctc catcacatcg
     2281 cgggggacgg ctggtcggtg gacctcttcc tgcgggagct ggcgctgctc taccacgcct
     2341 tcgccgaggg ccggccgtcg ccgctgcccg agttggcgat ccagtacgcg gactttgcgg
     2401 tctggcagcg gagctggctg gcgggagagg tgctggagag ccagatcgcc tactggcggg
     2461 agcgcctggc cggagcgccg gccgtcctcg acctgacgac ggaccgcccc cgtcccgccg
     2521 tcccgggcca ccgcggggag cgctggccgg tagagccgcc ggcagctctc gcagaggagc
     2581 tccgccggct cggccggcgg gaaggcgcca ctctcttcat ggtcgtgctg gccgccttcc
     2641 agaccctgct ggcgcgccac tccggtcagg gcgatgtggt gatcggcacg cccatcgcgg
     2701 ggcgcgaccg gctggagacg gaggagctga tcggattctt cgtcaacacc ctggcgctgc
     2761 gcatcgactc tgccggagac ccctccttcc gcgagctgct ggcgcgagta cgggaggtca
     2821 ccctgggcgc ctacacgcac caggagctgc ctttcgagcg tctggtcgaa acgctgcagc
     2881 cggagcggca cctcaaccgc tctccgctgt tccaggtgat gttcgcgttc cagaacgtgc
     2941 cgcagggcgg ccccgggatc ccgggactgg cgatctccgc gctaccggtc gacctggagg
     3001 tggcgaagtt cgatctcgtg ctgacgctcg gcgaatcggc cgccggcctc accgggacgc
     3061 tggagtacga cgcggatctg ttcgaccgct cgacggccgc gcggctggcc tcccacctca
     3121 atcgtttcct cgacgagatc gccgccgatc ccgcacggcg cgtcagcgaa gtgccgcagc
     3181 tcgctcccgc ggagcagcac cagttgctgg tcgagtggaa cgactccgcg gcggactacg
     3241 ccggggcggt ctgcctgcac gagctgatcg agtcccaggt ggagcgctcg ccggacgccg
     3301 tggcgctgtc gtgcggaggc gaggagctga gctacgccga gctggagagg cggtccaatc
     3361 tcctggctca ccgtctgcgc gagctgggag cggggcccga ggtgccggtg gcgatgtgcg
     3421 tcgagcgctc gctcgccatg ctggtgggcc tgctggcggt gctcaaggcg ggcggggcct
     3481 acgtgccgct cgatccggac tacccgtccg agcgcctggc gtccatgatc gaagactccg
     3541 gcgcgtctct cctgctcgcc cagagccacc tggtgccgcg gctgcccgcc ggcaacgccc
     3601 gggtgctgct gctggatcac gaggacggag ccgcggctcc cggcaccggc cggccgccgg
     3661 cgcggccgat cggcggcgcg atgccggaca gcctggtcta cgtcctcttt acctcgggct
     3721 ccaccggccg gcccaaaggc gcggcgatct ctcaccgcgc catcctcaac cggctgctct
     3781 ggatgcagga ggcgctgcgg ctctccgcca aggaccgggt gctgcaaaag accccgttcg
     3841 gcttcgacgt gtcggtctgg gagctgttct ggcccttcgt gaccggtgcc tgcctggtgg
     3901 tcgcccggcc cggcggccac cgcgacaacg cctacttggc gcacctgatc gccgaggagg
     3961 agatcacggt cctgcacttc gtcccctcca tgctgcagct tttcctggag gagagttcgg
     4021 atcggggtgg ggccggcggg tgcggcaccc tgcgcgacgt ggtgtgcagc ggcgaggcgt
     4081 tgccctccga gctggtccgg cgtttcgccg cccgcctcgg ccatgcccgg ctgcacaacc
     4141 tctatgggcc caccgaggcc gcggtggacg tcacctcctg ggtctgcgac gaggccggca
     4201 acgaccacgg tggcattccc atcgggcggc ccatcgccaa cacccgcatc cacctgctcg
     4261 accgcaacct gctgccggtg ccgttggggg tcgcgggcga gctgttcatc gccggcgtca
     4321 acctggcgcg tggctacatc gggcgtcccg acctcacggc agatcgcttc cttcccgacc
     4381 ccgaggccaa ggacccgggc gagcgcgtct accgcaccgg cgacctggcg cggtggcgcg
     4441 gcgacggtgc catcgagtac ctcggccgcc tggatcatca ggtcaagatc cgcggcgtcc
     4501 gcatcgagct gggcgagatc gaggcggcgc tgaccgagct gcccggagtg cgcgaggcgg
     4561 tcgtgatggc gcggtccgaa aagggggacg gcgacaagcg tctggtcgcc tatgtggtgg
     4621 gcgatgtccc ggccggcgag ctgcgccagg ccttgtggga gcgactgccg gaggccatgg
     4681 tgccctctgc cttcgtcaaa ctcgcggcgc tgcccctcaa cgccaacggc aaggtggacc
     4741 gcaaggcgtt gtctgccggg gccgtcccgg agcagcctgg cgcgggcgaa gaaggttacg
     4801 tggcgccgag aacacgcgag gaggagatcc tggcgggcgt ctggacgcag atcctgcgcc
     4861 tgccccgggt gggcgtgaac gacaacttct tcgagctcgg cggcgattcg atcctctcgg
     4921 tccagatcgt agcccgggcg cgtcaagccg gcctgctctt caccacgcgg caggtcttcg
     4981 agcatcagac cgtggccggg ctcgcccgcc acgccgaggt cgcggacgcc gcggctgtct
     5041 cccgtgccga gcagggaccg gtgacgggcg aggtgccgct ggccccgatg cagcagtggt
     5101 tcctcggcga cgatcccgtc gatgcgcacc atttcaatca atccctgctg ctccagtctg
     5161 gacaggccct ggacccggtc gccctcgagc aggtgatcgg gtatctgctg gcgcaccacg
     5221 atgcgctccg cctgcgcttc gtccgggagc gggagggctg gcgccagcgc aacgatcccc
     5281 ccggccgggt gcccttccat cgggtggacc tcacggggct ccctgctgcc cggcgggccc
     5341 cgtctctgga ggctgcggcc gcgcaggcgc aagcctgcct gaacctctcc gacgggccgc
     5401 tgctgcgcgc ggtcttcttc accttcggtg gcgagaaagg cgagcggttg ctgctggcgg
     5461 tccatcacct tgggatggac ggggtctcct ggcgcatcct gttgaccgat ctggagaccg
     5521 gctataccca gatcacgcga gggcaggctc tccgtctccc tcagaagacg acctccttca
     5581 agggttgggc ggagcggctg gccgggtatg cccgttcgca ggacctgcga agcgagttgg
     5641 gttactggct cgccggtccg cggatgcagg cggcgcgcct gccgctcgac ggcagcgggg
     5701 acgaggacgg ctggagcccg gccactctct ccatctccct gagcaccgag gagacacggt
     5761 gcctcgtgca ggaggtgcct gcggcgtcac gagcccacgt gcaggagctc ctgttggccg
     5821 ctctcgccct ggctctgcgc cgatggacgg gcaaggacgc cctgctgatc gacatggaag
     5881 ggcatggacg ggaggaggtc ttcggcgacg tcgatctgac gcgcaccgtc ggctggttca
     5941 ccaccctgtt cccgttgctg gtagacctgc agggagagag ggagccgggg gccgtcctgg
     6001 ccagagtgca gggccaggtc cgggcagtgc ccggccgcgg cttcggatat ggcgtgctgc
     6061 gctatctgtc ccctgatccc gaagtcgcgg agcggctagc ggtgctgccg ccggcgcaga
     6121 tcgttttcaa ttatctcgga cgtttcgacg gcgtggcgga gacctcgtcc ctcttccatc
     6181 tggcgcccga gtcgaccggt cctaccagca gcccgaggtt gaagcgtaag cacctcctgg
     6241 aggtcggcgc gtgggtgggc ggtggacggc tggtcgtgac cttcacctac ctcgagaccg
     6301 cccaccgcgt cgccaccatc gaagccttga cgggcgattt cctcgcatcg ttgcgcacgc
     6361 tgatcgcgct ctgcctggcc ggaggaccgc cggaggtgct gccgccggat ctcgcgctgg
     6421 gcatcgatcc gaaggagctc gaagaggcgt tgcacgagat tgagttcgaa ggatgggaaa
     6481 cgaatgagca agcgtaacgt cgagagcgcc tataccctgt cgccgctgca ggaggggatg
     6541 ctgttccact ccctctacga gcccgaggcg ggggtctacg tcacccagct cgggtgcgtc
     6601 ctggacggcc aactcgatct cgaagccttc acgcaggcat ggcggcggtt gatggagcgc
     6661 cactcgatcc tgcgcaccgc cttcgcctgg aagaacaccg gcaggccgct ccaggtggtg
     6721 ggccgcgagg tgcggctgcc cctggtgacg gaggactgga gcgatctccc atcggatgag
     6781 cgggagcgcc gctggcagga ctatctggcg gccgaccgga aacgcggtct ggatcccgcg
     6841 aaggcgccgt tgatgcggct cgccctggta cgggaggcgc cggacctcca ccgtttcctg
     6901 tggacccacc accacatcct cctggacggc tggtccatcg ccaaagtgtt cgaggagctc
     6961 ttcgtcctct acgaggctca tcgccagaag cgggacgtcg atcttcctct ctccccgccc
     7021 ttccgcgact acatcgtctg gctggggcgc caggacctga cgcaggccga agccttctgg
     7081 cgcgcggagc ttgccgggtt cagcgcgccc acgccgctgt ggccgggggc gtccgcgcca
     7141 tctcccacca aggagggcgc tgctgcggag gggcaggatc tgcagatccg gcagctgcca
     7201 gggtcgacgg tggacgcgtt gcgcgcgtgg gcccggagct gccagctgac tcccaacacc
     7261 ctggtccagg cggcctgggc actgatgctc gcacggtaca gcggcgagga ggacgtcgtc
     7321 ttcggcatca cggtctctgg caggtcgccc tccctccccg gggtggaggc catggttggg
     7381 ctgttcatca acactctgcc gttgcgcgtc gatgtgcgcc gcgggcagga gctgctgccc
     7441 tggttgcggc gcctgcagga gcggcaattt ggactgcggc agtatgagtt cagcccgctg
     7501 gtggagatcc agggctggag cgaggtcccg cgcggcctgc ctctgttcga gagcttcctg
     7561 gtcttcgaca actaccccgt ggagcgccgg gtgggcgaga acggaaatgg cctggaggtg
     7621 cgggacgtcc aagccgtcga gcatgtcagc tacccgctca gcctgacgtc cagctcggac
     7681 ctgcggctgc aagtcagcta tgacgccaag cgcttcgagg ccaccgcggt ctcccgtctg
     7741 ctcggtcatc tggagatgct gctggagagc ttcgtggccc ggccggaggg gcgtctcgag
     7801 gacatggtcc tgttgaccgg tccagagcgc aggcagttgc tggtggaatg gaacagcgcc
     7861 ggggagccgg tcgccgccgg ggagtgtctg cacgagttgt tcgaagccca ggccgcgcgg
     7921 attcccgacg cggtggcgtt ggcctgggag ggagagcggc tgacctaccg tgatctcgac
     7981 gtgcgggcga accggctggc gcgtcacctg cggcgcctcg gcgtcgggcc cgagatcccg
     8041 gtgggcgtct gcctgaggcg ctcgccggac ctcatggtga gcctgctggc gatcctcaag
     8101 gcgggtggtg cctatgtgcc gctcgatccg gagtatccgg cccagcgtct ggcggcgatg
     8161 gtgcctgcac tggtgatcgt ggcgagggac accgcggagt gcctgcccgt cagccagggg
     8221 atccggctcg tgcggctgga ggatgcggcc ctggccgtgg cggccgagcc cggagtggct
     8281 ccggcgccgc tggcggtgag cgggaacctc gcctatctcc tctatacctc cggttcgacc
     8341 ggagcaccca aaggggtcgc catcgagcac cacagcgtgg tggcgctgat cgattgggca
     8401 cgccgagcct tctcctccga cgatctggcg ggtgttctgg cttcgacctc gatcaacttc
     8461 gatctttcgg tcttcgagct gttcctgccg ctgagcaccg gaggaacggt ggtgctggcg
     8521 cgcgacgccc tgcatctggc cgagctggcc gaggccggcc aggtgagcct ggtgaacacc
     8581 gtcccttcgg ccatggcgga gctggtacgc ctggccgcgg tgcccgcgtc ggtgcgcacg
     8641 gtgaatctgg caggagagcc gctcagggca gagctggtgc gggagatcta ccgccagccg
     8701 acggtggacc gggtgctgaa cctctacggt ccttcggagg acacgaccta ctccacctgg
     8761 gcggaggtcc cgcggggttt cgaaggggca ccctcgatcg gacggtctgt cgcgggctca
     8821 cgggcctacc tgctggatcg ttggagggag ccggtccctc cgggtgtcca gggtgagctc
     8881 tacctggggg gcccgggact ggcgcgcgga tacttccggc aaccggcctg gacggcggag
     8941 aggttcgggc ccgatccctt ggccgatacc gagcccggga gccggttgta tcggaccggg
     9001 gatctggcgc ggttccggcc ggacggtcag atcgagttcc tcggccggac cgatcaccag
     9061 gtcaaggtgc gaggtttccg gatcgagctg cacgagatcg aggtggcgct cgccgcgcat
     9121 ccccagatcc gggaggcggt ggtggtggtg cgggaggaca cccccggcga
//
LOCUS       DASPJI010000340        14163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_828173, whole genome shotgun sequence.
ACCESSION   DASPJI010000340 DASPJI010000000
VERSION     DASPJI010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14163)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14163
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828173"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VEL74_08975"
     CDS             complement(<1..950)
                     /locus_tag="VEL74_08975"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HYG62701.1"
                     /translation="MNSPRLDIYCMAIGGTGMAPLACLLQEQGHAVRGTDGPLYPPMS
                     TLLEGAGIRPTVGYDPAHLEPRPDLVVIGNAIRRDNPEAVEVERQGIPYLSMPQALSR
                     FFLESRRPLVVAGTHGKTTTTAMAAWVYAQCGADPGFLIGGVPRDLPVSFHAGTGERF
                     VVEGDEYNAAYFDRGPKFLHYRPDTLILTSVEYDHADLYASPEALHAAYAELIRLLPP
                     TGYLVACGDTPKVRELAALARCPVLFYGVDQRGESNDLHPLGGIEAMEEGSRFRVRDV
                     DAGEVEISLRLVGGHNVSNALAVWAAARRDGLPADRVAAA"
     gene            1030..2067
                     /locus_tag="VEL74_08980"
     CDS             1030..2067
                     /locus_tag="VEL74_08980"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HYG62702.1"
                     /translation="MKGPLKNAVLASCAALLLALSAGAAAAAGPKAVAVEAIKDVGTL
                     PKGEKIVHDFVIRNEGDAVLQITEVRAACGCTVANYDKTIAPGQTGKVNVVIDTATFS
                     GAVAKGVTVLTNDPQTPEIELTVKAKLEPMISVKPGYARYITVQKEPKQGVIVQTLWA
                     PDGSDFDIVKVDSPYPFLTTTHREAKPEERQAVEGVPADKGKQWRVEMTLSNETAPVG
                     PLSDFVTVHTNHAKQKLVQIPISGFVRPVVTVTPPVADFGQIELKEPLRKAINIRNFA
                     TEPIKVTGIEEDMQGVDVALEPLNEGREYQVRVVLKPELGKGPFNGKLTIRTDSPKIP
                     VIAVDLKGTVL"
     gene            2251..2466
                     /locus_tag="VEL74_08985"
     CDS             2251..2466
                     /locus_tag="VEL74_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006165215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HYG62703.1"
                     /translation="MHRKPVESSAVSSVGYDPRSEVLEVEFRSGAVYDYFEVPRGVYE
                     SLMKASSKGRFVSRRIRDRFPFERRGS"
     gene            2622..4112
                     /locus_tag="VEL74_08990"
     CDS             2622..4112
                     /locus_tag="VEL74_08990"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HYG62704.1"
                     /translation="MKHFSRNGLLALALMALAVAPAAAGVYIPTKTADTRDGACTETD
                     CSLREAVIAANATAEPDVILLHSGVYVLGLAGAGEDAAATGDLDIADDLTLLGDGVGT
                     TTVEGAGIDRVFHILAGASVEIRDVTVAFGAAGAGQDGGGILNAGTLTLVRTVIAANQ
                     AAGGAGGGLRNQGDSAVLTVRQSLLEGNEAAQGGGIAAQGTMTLANVTITNNTASGSG
                     GGIYSYAGLDATINNATIVLNTATTQSGGGVFAESGAFTTVDYPLFTNSIIAANVAPA
                     NRDCSGAATSGGYNVLGVNGDCIDFQAVKGDKVGTSAAPLDARLSPLADNGGPLPTFA
                     LLANSPAVNAGNPAAPGSGGGACEATDQRGQARTDRCDIGAFERASAGCVSGTNTLCL
                     NNDRFRVTATFTPPQGAGGTARAVEFTSDSGYLTFFDPNNVEVTVKVLNGCGLNNRYW
                     VFLAGMTNVEVTVTVTDTLTGAVKTYANPQGRLFRTVADTAAFPCS"
     gene            4182..6662
                     /locus_tag="VEL74_08995"
     CDS             4182..6662
                     /locus_tag="VEL74_08995"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HYG62705.1"
                     /translation="MATTTAPAPSILERQELDEKTLNIYRQLGFLQADLDPLSRIEGE
                     PLPIEDSEPATAAHARRFYCGTVGVEFMHIPEPARRHWIQERMEGEPVQVDQGRILEQ
                     LVRAETFEQVIQNRYLGTKRFSIEGVESLLPLLDQMLEAAAAQRTRQVVLAMSHRGRL
                     NVMLHTVGRTAAELFAGFEDVDPRSILGGGDVKYHMGATGTHRGKNGHEVSIHLVSNP
                     SHLEAVDPVALGRVRAKQVRLGDGDQKQVFSIVLHGDAAFSGQGITAETLNLADLPAY
                     RVGGTVHVIVNNLIGFTTEPHSLQSSRYASDVAKRIPIPIFHVNGEDPDAVARVAAMA
                     VEYRFAFKSDVVVDLIGYRRHGHSEVDDPTITQPLLYKKIHAHPPVWKLYAERTGRQQ
                     EAERLVAQVKEEMNAAHQEATAIEKKPMLRTMPSYWDLYKGGRYDRSLEVPTGLPAAA
                     IAELTAPLTRWPEGFHIHEKIKRLLEQRAEMGNGKRPIDYGMAEALALASLVKQGVPV
                     RLTGQDSRRGTFNQRHSVLIDIENNSEYMPLAHVSPDQAWIEIYDSMLSELAVLGFEY
                     GFSRDFPEALVMWEAQFGDFANGAQIIVDQFVSAGEDKWGLLSGVVMLLPHGYEGQGP
                     EHSSARLERYLQLCGEDNIQVCQPSTAAQYFHMLRRQALRPWRKPLVVMTPKSMLRHK
                     LSSSTVEELGRDRFLNVIPDPQVQDAQRVILCTGKIAHELRQERERRKDTATAILVLD
                     QLYPFPKDELRAELERHPNVRDLVWVQEEPANMGALFFVQPRIERIAGRPMRSVKRSA
                     SGSPATGSAKAHGMEQQTLLTLAFSTGV"
     gene            6672..10466
                     /locus_tag="VEL74_09000"
     CDS             6672..10466
                     /locus_tag="VEL74_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG62706.1"
                     /translation="MSGHPHPADGSLAFLSGGGDMGARMRALDWRRTPLGPPEGWPQS
                     LKTAVRIMLTSRQPIWIGWGEQLTYLYNDAYKSIVGGKHPGALGQPVSAVWREIWDEI
                     APMLSTAMTGDEGTYVEEQLLVMERNGYPEETYYTFSYSPIPEDGAGGGRAGGIICAN
                     TDDTRRVIGERQIALLRELAAGTAEARTWEEACERSARALASNPWDILFALIYMADPG
                     DRSVSLAGACGLEAGLAAALRGAPWPIEEVLRTHEPQLVSGLGAALGTGLPTGAWRQP
                     SSRAAVLPIAAAGETGRPGVLVVGLNPFRQFDDGYQGFLTLAAGQISASIGNARAYEE
                     ERRRAEKLAELDRAKTTFFSNVSHEFRTPLTLMLGPIEDILAKPEPEVLADNRRLLAL
                     VQRNGLRLQRLVNMLLDFSRIEAGRVQASYEPADLSAFTRELASTFRSACEKAGLELT
                     VDCPPLPAPVYVDRDMWEKVVLNLVSNAFKFTLEGGIEVALRAVDGGARLTVRDTGAG
                     IPPAEMPKLFERFHRVAGTPGRTQEGTGIGLALVQELVRLHGGEVRAESALGRGTTFF
                     VTLPFGKDHLPADRVGAARTLASTTVGAAPFVEEALRWIPPTPDAGSEWRPLDASLIH
                     VGPRPAGDGTRPRILLADDNSDVRDYIRRLLSLEYEVTAVADGVAALHAARRERPDLV
                     LTDVMMPGLDGFGLLRELRADAGTRSLPIIMLSARAGEESRVEGMEAGADDYLVKPFS
                     ARELLARVGAHLQMARLRREAAEALRESHARFEAVVDAAPIGIYFVDSGLKIRQVNPK
                     ALPVFGDIENLIGSDLADVMRILWTQPYADEVVEWFRHTLETGEPYFVPERIEERCDR
                     KVLEYYEWQTHRISLPDGQHGVVCYFNDISRHVLARQALAEADRRKDEFLATLAHELR
                     NPLAPIRNMLEIMKRTDGDARLVEQARATMERQLNHMIRLIDDLLDVSRISRGKVELK
                     RERVELASAVYQAVEACRPLAQAARHEVEVVLPPDPVYLDADPVRLAQIFGNMLNNSC
                     KYTDPGGKIRLTAERRDGDVLVKIRDNGLGIPPDKLGSIFEIFTQVDRTLERSQGGLG
                     LGLTLVKRLVEMHGGTVAAHSDGPGRGSEFVVRLPALQEQRAGEPSAEPDVPKATAGH
                     RILVVDDNQDSALSLAMLLEISGHEVRTAYDGLEAIDAAAEFRPDIVLMDVGMPKLNG
                     YDAARRIRAEPWGERMLLVAVTGWGQEEDRRRTTEAGFDGHLVKPVDFGAVTKLLDSM
                     RG"
     gene            10719..11117
                     /locus_tag="VEL74_09005"
     CDS             10719..11117
                     /locus_tag="VEL74_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62707.1"
                     /translation="MKRETTYTGIMGELRRTLDAFTANAGGELGYLETQRNRVSTLLD
                     RAFELARQQAALTASRQDVSKRLKATIEEGLRALTVLRVSLKDHYGPRSEKLVEFGIQ
                     PFRGRKPKTEVPAVEQQGPDAGPSTPSSDS"
     gene            11658..12227
                     /locus_tag="VEL74_09010"
     CDS             11658..12227
                     /locus_tag="VEL74_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPTL-CTERM sorting domain-containing protein"
                     /protein_id="HYG62708.1"
                     /translation="MRLSFASAAVALFLAFAAPAAGQVTYSYQGNPFTTASAPYTTSM
                     SISGSFEVGVALAPSSTIDLSGSLVGFSFFDGVETRDQTNSLTCQFEVTTNAAGAITG
                     WNIWLRESGGADPQHSVETRSTTDLAGFLSPNSACGAGALNPFASTVAQPGVWSGGPR
                     IVVVPTASEIGLVLLTALLAACAVWRLRF"
     gene            12428..14092
                     /locus_tag="VEL74_09015"
     CDS             12428..14092
                     /locus_tag="VEL74_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYG62709.1"
                     /translation="MRRKPNFMIVITDQQSGKPYWPEGWADKNLPAMRWLKERGISFN
                     RAYTNSCTCSPSRVTMLTGLYPAQHGVTEVLEFDNVASVASDLTAESGQVSGLAMNIK
                     ERRQRGMVSNLQNLAKVLKTAGYNVIYKGKWHLTKPANFVNDPKNGIYQKYWTPDDAR
                     QLAERYGFDGWTVPDAGDNLAIENMGGGATKNDQRFVDGRGKAAKYGDVPYDLLEPHS
                     VVRFLETYDSDKPFCLIVSLVNPHDVLAYPGNPVGRTGEPIYQAAGYDLAKFQDLPID
                     RPPTWNEDLSTKPLSQTQFRALANAGNGPITADDTDLQLNYCRFYAYLCKHVDHQILQ
                     VLHALEKSRHYDDTVVVRISDHGDMAMSHGLQRQKMFNMYEETLNIPFVISHPHMFKH
                     CAGTETDSLASLIDLLPTLSNLAGVPDLDRWVFKGKDLTPILSNPKAEVQSYIHFTYE
                     DLYFYVAAANHIRAIIEGRWKYAVYYDIFTDQPFEYEMYDLAVPLDQREVRNLGHPDT
                     PVTAEEKAERERLHQKLIHVMETLGTLPHEIEWPKHWGEQRPPYSG"
BASE COUNT         2299 a         5027 c         4794 g         2043 t
ORIGIN      
        1 aaggcggcgg ccacgcggtc ggccggcagg ccgtcgcggc gggcggcggc ccagaccgcc
       61 agcgcgttcg agacgttgtg cccgcccacc agccgcagcg agatctccac ctctcctgcg
      121 tcgacatcgc gtacccggaa gcggctcccc tcctccatgg cctcgatccc gcccaggggg
      181 tggaggtcgt tgctctcacc cctctgatca acgccataga agaggacggg gcagcgggcg
      241 agggcggcca gctcccgcac cttcggggta tcgccgcagg cgaccaggta gcccgtgggg
      301 gggagcagcc ggatcagctc cgcgtaggcg gcgtgcagcg cctccgggct cgcgtagagg
      361 tcggcgtggt cgtactcgac gctggtgagg atcagcgtat ccggccggta gtggaggaac
      421 ttggggccgc ggtcgaagta ggcggcgttg tactcgtccc cctcgacgac gaagcgctca
      481 ccggtgccgg cgtggaagct caccggcagg tcgcgcggca cgccgccgat gaggaagccg
      541 gggtcggcgc cgcactgcgc gtagacccag gccgccatgg ccgtggtcgt ggtcttgccg
      601 tgggtccccg ccaccaccag ggggcggcgg ctctccagaa agaaccggga gagcgcctgg
      661 ggcatggaga ggtaggggat gccctgccgc tccacctcca cggcctcggg gttgtcgcgg
      721 cggatcgcgt tgccgatgac caccaggtcg gggcgcggct ccaggtgggc cgggtcgtag
      781 ccgacggtcg gccggatgcc ggcgccttcg agcagcgtgc tcatcggcgg gtagaggggg
      841 ccgtcggtgc cgcggacggc gtggccctgc tcttgcagca ggcaggcgag cggagccatc
      901 ccagtaccgc cgatcgccat gcagtaaatg tcgagtcgag gggaattcac gcgcgggtct
      961 atctcgttca agggtgccga gtgtagcatt cccccagtct gggtccacat tcccgaagga
     1021 gttggtcgta tgaaaggtcc tctgaagaac gccgttttgg catcctgtgc cgcgctgctc
     1081 ctggcacttt cggcaggcgc cgcggcggcg gccggcccca aggccgtggc cgtcgaagcc
     1141 atcaaggacg tgggcacctt gccgaagggc gagaagatcg tccacgactt cgtcatccgg
     1201 aacgaagggg acgcggtcct ccagatcacc gaggtgcgcg ccgcctgcgg ctgcaccgtg
     1261 gccaactacg acaagacgat cgcgcccggc cagaccggca aggtcaacgt ggtgatcgac
     1321 accgccacct tcagcggcgc cgtcgcgaag ggggtgacgg tgctcaccaa cgatccgcag
     1381 acgccggaga tcgagctcac cgtcaaggcc aagctggagc ccatgatctc agtcaagccc
     1441 ggctatgccc ggtacatcac cgtgcagaag gagcccaagc agggcgtcat cgtccagacg
     1501 ctgtgggcgc cggacggcag cgacttcgac atcgtcaagg tggactcccc ctatccgttc
     1561 ctcaccacga cccaccgcga ggccaagccc gaggagcgcc aggcggtcga gggcgtcccc
     1621 gcggacaagg gcaagcaatg gcgggtggag atgacgctgt cgaacgagac cgcgcccgtc
     1681 gggccgctct ccgacttcgt caccgtgcac accaaccacg ccaagcagaa gctggtgcag
     1741 atcccgatct cgggcttcgt ccggccggtg gtgacggtga cgccgccggt ggccgacttc
     1801 ggccagatcg agctcaagga gcctctccgc aaggcgatca acatccgcaa cttcgccacc
     1861 gagccgatca aggtgaccgg tatcgaggag gacatgcagg gcgtcgacgt cgccctcgag
     1921 ccgctgaacg agggacgtga gtaccaggtg cgcgtcgtcc tcaagccgga gctgggcaag
     1981 ggcccgttca acggcaagct caccatccgc accgacagcc ccaagatccc ggtcatcgcc
     2041 gtggacctca aggggaccgt gctgtaggcc cggcccttct cgacccttcc tgacggccgg
     2101 cgccctccga gggcgccggc cgtttttgct gacggcaaac tacttttgac ggcctgaagc
     2161 ttcagcctca ggccgtggta tcccggttgc agcacttgtc gcttcaggcg gagacgcggg
     2221 gacgtctttg cggcctggag gactggtgcc atgcacagga agcccgtgga gtcctcggcg
     2281 gtctcctcgg tgggctacga cccgcgctcg gaggtcctgg aggtcgaatt ccggagcggc
     2341 gcggtctacg actacttcga ggtaccgcgc ggagtctacg agtcgctgat gaaagcctcg
     2401 tcgaagggcc ggttcgtctc ccgccgcatc cgggaccggt tccccttcga gcgccgggga
     2461 agctgaccgg tccatcctcc ttgggatagc ctcctcgtgg cctccttggg acggcacctg
     2521 gacgttcgtg ccgtcccgtc cgtaaacaag ataggagaca ttgagacttg ccctggagac
     2581 cgataggctc cgaggccctg agatccacca aggaggacat catgaagcat ttcagccgta
     2641 acggcctgct cgccctcgcc ctgatggcgc tcgccgtcgc ccccgccgcc gcgggggtct
     2701 acatccccac caagaccgcc gacacgcgcg acggcgcctg caccgagacc gactgctcgc
     2761 tgcgcgaggc ggtcatcgcc gccaacgcca ccgccgagcc cgacgtcatc ctgctgcact
     2821 ccggcgtcta cgtcctgggg ctcgcgggag cgggcgagga cgccgccgcc accggcgacc
     2881 tggacatcgc ggacgacctg accctcctgg gtgacggcgt cggcaccacc accgtcgagg
     2941 gcgccggcat cgaccgcgtc ttccacatcc tcgccggcgc gagcgtcgag atccgcgacg
     3001 tgaccgtcgc cttcggcgcg gccggcgccg gccaggacgg cggcggcatc ctcaacgccg
     3061 gcacgctgac cctggtgcgc accgtgatcg ccgccaacca ggcggccggc ggcgccggcg
     3121 gcggcctccg caatcagggg gattcggccg tgctcaccgt gcgccagagc ctgctcgagg
     3181 gcaacgaggc cgcccagggc ggcggcatcg cggcccaagg cacgatgacg ctcgccaacg
     3241 tcacgatcac caacaacacc gcctcgggct cgggcggcgg catctactcc tacgcgggcc
     3301 tggacgcgac gatcaacaac gccaccatcg tgctgaacac cgcgacgacg caatcgggcg
     3361 gcggcgtctt tgccgagagc ggcgccttca ccaccgtcga ctacccgctg ttcaccaact
     3421 cgatcatcgc cgccaacgtc gcccccgcca accgtgactg ctcgggcgcg gcgacctcgg
     3481 gcggctacaa cgtcctgggg gtcaacggcg actgcatcga cttccaggcc gtcaaggggg
     3541 acaaggtcgg cacctccgcg gcgccgctcg acgcccggct ctccccgctc gccgacaacg
     3601 gcggcccgct gccgaccttc gccctgctgg ccaacagccc ggcggtcaac gccggcaacc
     3661 cggccgcgcc gggcagcggc ggcggcgcct gcgaggcgac cgaccagcgc ggccaggcgc
     3721 ggaccgaccg ctgcgacatc ggcgccttcg agcgcgccag cgccggctgc gtctccggca
     3781 ccaacactct gtgcctgaac aacgaccgct tccgggtgac cgccaccttc acgccgccgc
     3841 agggcgccgg cggcaccgcc cgcgccgtcg agttcacctc cgactccggc tatctgacct
     3901 tcttcgaccc caacaacgtc gaggtcaccg tcaaggtgct gaacggctgc ggcctcaaca
     3961 accgctactg ggtgttcctc gccggcatga ccaacgtcga ggtcacggtg acggtcaccg
     4021 acaccctgac gggcgcagtc aagacctacg ccaacccgca gggccgcctg ttccgcaccg
     4081 tcgcggacac cgccgccttc ccctgctcgt agcgctcctc cctcccgccg cccgtctccg
     4141 cgggcggcgg gtcctttcct ggtggtatcc tgaccggtgc tatggctaca acgaccgcac
     4201 ccgcaccgtc cattctcgag cgccaggagc tcgacgagaa gactctcaat atctaccgcc
     4261 agctcggctt cctgcaggcc gacctcgacc cgctgagccg gatcgagggg gagccgctgc
     4321 cgatcgagga cagcgagccg gccaccgccg cccatgcccg ccgcttctac tgcggcaccg
     4381 tcggcgtgga gttcatgcac atccccgagc ccgcgcgccg gcactggatc caggagcgga
     4441 tggaagggga gcccgtccag gtcgaccagg ggcgcatcct cgagcagctc gtgcgcgccg
     4501 agaccttcga gcaggtgatc cagaaccgct acctcggcac caagcgcttc tcgatcgagg
     4561 gggtcgagtc gctgctgccg ctcctcgacc agatgctcga ggccgcggcc gcccagcgga
     4621 cgcggcaggt ggtgctggcg atgagccacc gcggccgcct gaacgtgatg ctccacaccg
     4681 tcggccgcac ggccgcggag ctcttcgccg gcttcgagga cgtggacccc aggagcatcc
     4741 tgggcggtgg cgacgtcaag taccacatgg gcgccaccgg cacccaccgc ggcaagaacg
     4801 gccacgaggt gtcgatccac ctggtgtcga accccagcca cctcgaggcg gtcgacccgg
     4861 tcgccctcgg ccgggtgcgg gccaagcagg tccgcctcgg cgacggcgac cagaagcagg
     4921 tcttctccat cgttttgcac ggcgacgccg ccttctccgg ccaggggatc acggcggaga
     4981 cgctgaacct ggcggacctc ccggcctacc gggtgggagg cacggtgcac gtcatcgtca
     5041 acaacctgat cggtttcacc accgagccgc acagcctcca gtcgtcccgc tacgcctcgg
     5101 acgtggcgaa gcgcatcccg atcccgatct tccacgtcaa cggcgaggac cccgacgccg
     5161 tggcgcgcgt ggcggcgatg gcggtcgagt accggttcgc cttcaagagc gacgtggtgg
     5221 tggacctcat cggctaccgc cggcacggcc acagcgaggt ggacgacccg acgatcaccc
     5281 agccgctcct ctacaagaag atccacgcgc acccgccggt ctggaagctg tacgccgagc
     5341 gtaccggccg gcagcaggag gccgagcgcc tggtcgcgca ggtgaaggag gagatgaacg
     5401 ccgcccacca ggaggcgacg gcgatcgaga agaagccgat gctgcggacc atgccctcct
     5461 actgggacct ctacaagggc gggcgctacg accgctcgct cgaggtgccg acggggctgc
     5521 cggcggcggc gatcgccgag ctgaccgcgc cgctcacccg ctggcccgag gggttccaca
     5581 tccacgagaa gatcaagcgc ctgctggagc agcgggcgga gatggggaac ggcaagcggc
     5641 ccatcgacta cggcatggcg gaggcgctgg cgctcgcctc gctggtcaag cagggggtgc
     5701 cggtccgcct caccggccag gacagccgcc gcggcacctt caaccagcgg cactcggtgc
     5761 tgatcgacat cgagaacaat tcggaataca tgccgctcgc tcatgtttcg ccggaccagg
     5821 cgtggatcga gatctacgac tcgatgctct ccgagctcgc cgtcctcggc ttcgagtacg
     5881 gcttctcgcg cgacttcccg gaggcgctgg tgatgtggga ggcgcagttc ggcgacttcg
     5941 ccaacggcgc gcagatcatc gtcgaccagt tcgtcagcgc gggcgaggac aagtgggggc
     6001 tgctgtcggg ggtcgtgatg ctcctccccc acggctacga ggggcagggg ccggagcact
     6061 cgagcgcccg cctggagcgc tacctccagc tctgcggcga ggacaacatc caggtctgcc
     6121 agccgtcgac cgcggcgcag tacttccaca tgctgcggcg gcaggcgctg cgcccctgga
     6181 ggaagccgct ggtggtgatg accccgaaga gcatgctgcg ccacaagctc tcctcctcga
     6241 cggtggagga gctcgggcgc gaccgcttcc tgaacgtcat ccccgacccg caggtgcagg
     6301 acgcccagcg ggtgatcctc tgcaccggca agatcgccca cgagctgcgc caggagcggg
     6361 agcgccgcaa ggacaccgcg acggccatcc tcgtcctgga ccagctctac cccttcccga
     6421 aggacgagct gcgggcggag ctggagcgcc acccgaacgt ccgcgacctc gtctgggtgc
     6481 aggaggagcc ggcgaacatg ggcgccctct tcttcgtcca gccgcgcatc gagcgcatcg
     6541 ccggccgccc catgcgctcg gtgaagcgct ccgcctcggg gagcccggcc accggctcgg
     6601 ccaaggcgca cggcatggag cagcagacgc tgctgacgct ggcgttctcg acgggggtct
     6661 gagcccggca cgtgagcggg cacccccatc ctgcggacgg cagcctcgcc ttcctctccg
     6721 gcggcggtga catgggcgcg cggatgcgcg ccctcgactg gcggcgcacg ccgctgggcc
     6781 cgccggaggg ctggccgcag agcctgaaga cggcggtccg catcatgctc acctcgcgcc
     6841 agcccatctg gatcggctgg ggcgagcagc tcacctatct ctacaacgac gcctacaagt
     6901 ccatcgtcgg cggcaagcac ccgggagcgc tggggcagcc ggtctcggcc gtgtggcgcg
     6961 agatctggga cgagatcgcc ccgatgctct cgaccgcgat gaccggggac gaggggacct
     7021 acgtcgaaga gcagctcctc gtcatggagc gcaacggcta tcccgaggag acctactaca
     7081 cgttctccta cagccccatc ccggaggacg gggcgggggg cgggcgggcc ggcgggatca
     7141 tctgcgccaa cacggacgac acccggcgcg tcatcggcga gcgccagatc gcgctgctgc
     7201 gcgagctggc cgccggcacc gccgaggccc gcacctggga ggaggcctgc gagcggagcg
     7261 cccgcgcgct ggcgagcaat ccctgggaca tcctcttcgc gctgatctac atggccgatc
     7321 ccggagaccg gagcgtttcg ctggcgggcg cctgcggtct cgaagcgggc cttgcggcgg
     7381 ctctccgcgg cgccccctgg cccatcgaag aggtgctgcg gacgcatgag ccgcagctgg
     7441 tctccggcct cggagcggcg ctcggcaccg ggctgcccac cggcgcctgg cggcagcctt
     7501 cgtcccgggc cgcggtgctg ccgatcgcgg ccgcgggaga gaccggccgg ccgggcgtgc
     7561 tcgtggtcgg tttgaatccg ttccggcagt tcgacgacgg ctaccagggg ttcctcacgc
     7621 tcgccgccgg gcagatctcc gccagcatcg gcaacgcccg cgcctatgaa gaggagcggc
     7681 ggcgggcgga gaagctggcc gagctggacc gggcgaagac cacgttcttc tccaacgtga
     7741 gccatgagtt ccgcacgccc ctcacgctga tgctcggccc gatcgaggac atcctggcca
     7801 agccggagcc ggaggtcctg gcggacaacc ggcggctcct cgcgctggtg cagcgcaacg
     7861 gcctgcggct gcagcggctg gtgaacatgc tgctggactt ttcgcgcatc gaggcggggc
     7921 gggtccaggc ctcgtacgag ccggccgacc tgtcggcgtt cacccgcgag ctggcgagca
     7981 ccttccggtc ggcctgcgag aaggcgggcc tggagctcac ggtcgactgc ccgccgctgc
     8041 ccgcgccggt ctacgtcgac cgcgacatgt gggagaaggt cgtcctcaac ctcgtctcca
     8101 acgccttcaa gttcacgctc gaagggggca tcgaggtcgc cctgcgggcg gtggacggcg
     8161 gcgcgcggct gaccgtgcgc gacacggggg ccggcatccc gcccgcggag atgccgaagc
     8221 tgttcgagcg cttccaccgg gtcgcgggga cgccgggccg gacgcaggag ggcaccggga
     8281 tcggcctggc tctcgtccag gagctggtgc ggctccacgg cggcgaggtg cgggccgaga
     8341 gcgccctggg ccggggcacg acgttctttg tcaccctccc cttcggcaag gaccatctgc
     8401 cggccgaccg cgtcggcgcc gcccgcaccc ttgcctccac gacggtgggg gccgcgccct
     8461 tcgtcgagga ggccctgcgc tggatccccc ccaccccaga cgccgggtcc gagtggcggc
     8521 cgctggacgc gtcgctgatc cacgtggggc cgcgcccggc aggagacggc acccgccccc
     8581 gcatcctgct ggccgacgac aacagcgacg tgcgggacta catccgccgg ctcctcagcc
     8641 tggagtacga ggtgacggcg gtggccgacg gggtggccgc gctccatgcg gcgcgccggg
     8701 agcggcccga cctggtcctc acggacgtga tgatgcccgg cctcgacggc ttcgggctcc
     8761 tccgcgagct gcgggccgac gccggcacga ggagcctgcc gatcatcatg ctgtcggccc
     8821 gcgccggtga agagagccgg gtcgagggga tggaggcagg ggccgacgat tacctggtga
     8881 agccgttcag cgcccgcgag ctgctggcgc gcgtcggggc gcacctgcag atggcgcggc
     8941 tgcgccgcga ggccgcggag gccctgcgcg agagccacgc ccgcttcgag gccgtcgtcg
     9001 atgccgcgcc gatcgggatc tatttcgtgg attccggtct gaagatccgg caggtgaacc
     9061 cgaaggcgct gcccgtgttc ggcgacatcg aaaacctgat cggcagcgac ctcgcggacg
     9121 tcatgcgcat cctctggacg cagccgtacg cggacgaggt ggtggagtgg ttccgccaca
     9181 cgctcgaaac cggcgagccg tacttcgtcc ccgagcgcat cgaggagagg tgcgaccgca
     9241 aggtgctgga gtattacgaa tggcagaccc accgcatctc gctgcccgac gggcagcacg
     9301 gagtggtctg ctacttcaac gacatctccc ggcacgtcct ggcccggcag gcgctcgccg
     9361 aagccgaccg ccgcaaggac gagttcctgg cgacgctggc gcacgagctg cgcaacccgc
     9421 tcgcgcccat ccgcaacatg ctggagatca tgaagcggac ggacggcgac gcgcgcctgg
     9481 tcgagcaggc ccgcgccacc atggagcgcc agctgaatca catgatccgg ctcatcgacg
     9541 acctgctcga cgtgagccgc atcagccggg gcaaggtcga gctgaagcgg gagcgggtcg
     9601 agctggcgtc ggcggtgtac caggcggtcg aggcgtgccg cccgctcgcc caggccgccc
     9661 gccacgaggt cgaggtcgtg ctgccgccgg accccgtcta tctggacgcc gatccggtac
     9721 ggctggcgca gatcttcggc aacatgctca acaactcctg caagtacacc gatccgggag
     9781 ggaagatccg gctgaccgcc gagcgccggg acggtgacgt gctggtgaag atccgggaca
     9841 acggcctcgg catcccgccc gacaagctgg gcagcatctt cgagatcttc acgcaggtgg
     9901 accggacgct ggagcggtcg cagggcgggc tcggcctcgg cctgacgctg gtcaagcggc
     9961 tggtcgagat gcacggcggc acggtggccg cccacagcga cgggccgggc cgcgggagtg
    10021 aattcgtggt gcgcctgccg gcactccagg agcagcgggc cggtgagccg tcggcggagc
    10081 cggacgtgcc gaaggccacg gcgggccatc gcatcctggt ggtcgacgac aaccaggact
    10141 ccgcgctcag cctggccatg ctgctggaga tcagcggcca cgaggtccgt accgcctacg
    10201 acggcctgga ggcgatcgac gcggcggcgg agttccggcc ggacatcgtg ctgatggacg
    10261 tcgggatgcc gaagctcaac ggctacgacg ccgcacgacg gatccgcgcc gagccctggg
    10321 gcgagcgcat gctcctggtc gcggtcaccg gctggggcca ggaagaggac cgccgccgca
    10381 ccaccgaggc cggcttcgac ggccacctgg tcaagccggt ggacttcggc gcggtgacga
    10441 agctgctcga ctccatgcgg gggtagggcg gtcccccctc cccgcttccg ggcgcggcac
    10501 ctccccccgt gccgcgccgg tacgtgtcct tcagttgtca aagagcccac ggatcgatcg
    10561 tatcggatcg aacgccgcga ggctaccacg ggacagcggc ttccgcaaga ctgattgacc
    10621 ataaacggtc ctcgtgctct atacttcctc tctcggcgcc atggaggcgc caggtttgag
    10681 ccggaacggc tcgttcgaga aaggaagaag gtatggctat gaagagagag acgacgtaca
    10741 cgggcatcat gggggagctg cggcggaccc tcgacgcgtt caccgccaac gcgggtgggg
    10801 agctgggcta cctggagacg cagcgcaacc gggtgtccac cctgctggac cgcgccttcg
    10861 agctcgcccg ccagcaggcg gccctgacgg cgagccgcca ggacgtgtcc aagcggctca
    10921 aggcgacgat cgaggagggg ctgcgcgcgc tgacggtgct gcgggtctcg ctgaaggacc
    10981 actacggccc gcgctcggag aagctggtcg agttcgggat ccagcccttc cgcggccgca
    11041 agccgaagac ggaggtgccg gcggtcgagc agcaggggcc ggacgccggc ccgtccactc
    11101 cctcctccga cagctgatcc cctgatcccg acgcttcacc ggggggcgcg caccgcgcgc
    11161 cccctttctt tgtctcgccg tcttctcccc cgcctctcct cccgcgtcga cagctcatcg
    11221 ccatctccga cggcatggcg gagggttccg tcgtcttgcc ggagtttccg tggccctgcc
    11281 ggaggcttcg gcgagttgcc ggaggctccg ggggcctgcc ccaacctccg atgcgctggt
    11341 ggagtgcccg gcaggacgcc ggaatcctcg gcgcgctgcc ggagatcccc gtaggccgtc
    11401 gcaggccccg gcgtgcgtgc agacgatccg tggacccctc gaaggctccg gcgcctcacc
    11461 agaggttgcg acgagccggc gggatctgcg gcgccggcgc gtaagcctcg taatatcgca
    11521 taagcgcaac cgaagtgtct ccgtcttcgc agaagaggga gacgagggat tgtgctcgcg
    11581 tcattcccgc actatgatcc gctcatctcg gtcgttcagc cgtttgctgt cccatgctcg
    11641 acgaaaaggg gggagtcatg cgtttgtcgt tcgccagtgc tgctgtcgcc ttatttcttg
    11701 ccttcgcggc tccggccgcc gggcaggtca cctattccta tcaggggaac ccgttcacca
    11761 ccgcctcggc cccctacacg acgtccatga gcatctcggg gagcttcgag gtgggcgtcg
    11821 cgctggcgcc gagctcgacc atcgacctca gcggctccct cgtcggcttc agctttttcg
    11881 atggggtgga aacgcgcgat cagacgaaca gcctcacctg tcagttcgag gtgaccacga
    11941 atgccgcagg tgccatcacg ggctggaaca tctggctccg cgagtccggc ggggccgatc
    12001 cgcaacacag cgtggagacc cggagcacga ccgacctggc aggcttcctg agcccgaaca
    12061 gcgcctgtgg cgcgggagct ctcaacccgt tcgcctcgac cgtggcccag ccgggggtgt
    12121 ggagtggagg gccgaggatc gtcgtggtgc cgacggcctc ggagatcggc ctcgtcctcc
    12181 tcacggcgct gctagccgcc tgcgcggtct ggcgccttcg gttctagagc gggtccttct
    12241 cctccgctcc gtccgtggtg ccgaggacgg gcggagcccc tcgcgagccc ttcctgacgt
    12301 cgggtgccct ctcccgatcg cctgtcgtat agtcgccccc gctaaacaga gacagaggcc
    12361 agggcaccgg acgggcaagg ctgccgtccg tgctggagcc tctcagacat tgaccagggg
    12421 aggactcatg cgacgcaagc cgaacttcat gatcgtcatc acggaccagc agagcggcaa
    12481 gccgtactgg ccggagggct gggcggacaa gaacctgccg gccatgcgct ggctcaagga
    12541 gcgcgggatc agcttcaacc gcgcctatac caactcctgt acctgctcgc cctcgcgtgt
    12601 caccatgctg accggcctct accccgccca gcacggcgtc accgaggtgc tcgagttcga
    12661 caacgtcgcc agcgtcgcct cggacctgac ggccgagtcg gggcaggtgt ccggcctcgc
    12721 gatgaacatc aaggagcggc gccagcgcgg catggtcagc aacctccaga acctcgccaa
    12781 ggtgctgaag accgccggct acaatgtgat ctacaagggc aagtggcacc tcaccaagcc
    12841 cgccaacttc gtcaacgatc ccaagaacgg gatctatcag aaatactgga cgccggacga
    12901 cgcgcggcag ctcgcggagc gctacggctt cgacggctgg accgtgcccg acgccggcga
    12961 caacctggcg atcgagaaca tgggcggcgg cgccacgaag aacgatcagc ggttcgtcga
    13021 cggccgcggc aaggccgcca agtacggcga cgtgccctat gatctgctcg agccgcacag
    13081 cgtcgtccgc ttcctcgaga cctacgactc cgacaagccg ttctgcctca tcgtctcgct
    13141 ggtcaacccg cacgacgtcc tcgcctatcc gggcaacccc gtgggcagga cgggagagcc
    13201 gatctatcag gccgcgggct atgatctggc gaagttccag gacctgccca tcgaccggcc
    13261 gccgacctgg aacgaggacc tctccaccaa gccgctcagc cagacgcagt tccgcgccct
    13321 ggccaacgcc ggcaacggtc cgatcaccgc cgacgacacc gacctgcagc tcaactattg
    13381 ccggttctac gcgtatctct gcaagcacgt cgaccaccag atcctgcagg tgctccacgc
    13441 gctcgagaag tcccgtcact acgacgacac ggtcgtcgtc cgcatcagcg atcacggcga
    13501 catggccatg tcgcacggcc tgcagcgcca gaaaatgttc aacatgtatg aggagacgct
    13561 gaacatcccg ttcgtgatct cccaccccca catgttcaag cactgcgcgg ggaccgagac
    13621 cgactcgctg gccagcctga tcgatctgtt gccgacgctc tccaacctcg ccggcgtccc
    13681 cgacctggac cgctgggtgt tcaagggcaa ggacctgacg ccgatccttt ccaatcccaa
    13741 ggccgaggtg cagagctaca tccacttcac ctacgaggat ctctacttct acgtcgccgc
    13801 cgcgaaccac atccgggcga tcatcgaggg gcgctggaag tacgcggtct actacgacat
    13861 cttcaccgac cagccgttcg agtacgagat gtacgacctg gccgtgcctc tggaccagcg
    13921 ggaggtgcgg aacctggggc atccggacac gccggtcacc gcggaggaga aggccgagcg
    13981 cgagcgcctg caccagaagc tgatccacgt catggagacg ctcggcaccc tgccgcacga
    14041 gatcgagtgg cccaagcact ggggcgagca gcggccgccc tacagcggct gatccccggg
    14101 cttcatccct gggctcggcg gccggacccc tgtagaatga ggcccgctct aaaggagggg
    14161 atc
//
LOCUS       DASPJI010000341         3297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_193846, whole genome shotgun sequence.
ACCESSION   DASPJI010000341 DASPJI010000000
VERSION     DASPJI010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193846"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VEL74_09020"
     CDS             complement(<1..527)
                     /locus_tag="VEL74_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HYG62710.1"
                     /translation="MATEVVMPQMGESIAEGTITKWLVKVGDRVERDQPLFEISTDKV
                     DAEIPSPGSGVLLEIRNQEGETVPVNQVVAVIGEAGEQPGAGAAAPAATGPAQQPEAV
                     AAAAPPESTEEIKQVEAAREPGAAATDSAPKVASPEPQPAPRQEAQPAAGSTADREQS
                     VRRDSGESSQAKESEG"
     gene            complement(555..1529)
                     /locus_tag="VEL74_09025"
     CDS             complement(555..1529)
                     /locus_tag="VEL74_09025"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HYG62711.1"
                     /translation="MAVVTYLEAIRQALWEEMEQDERVFMLGQDIGTYGGAFRVTSGF
                     LERFGPERIIDTPISESAMVGCAIGASMMGLRPVVEMQFIDFIACSFNQMVNFAAPNH
                     YRWGQSVPIVVRGPSGGNVHGSAFHSQNPEAFFHHAPGLKIVSPATPADARGLLKAAI
                     RDPNPVLFCEHKYLYRRIKEELPAGDGLVPIGKARLAREGRHLSVITWGAMLHVALEA
                     AGRLEADGIDLEVLDLRTIKPLDQEAILATVRKTSRVIVLTEEQLTGSVAGEVASRIS
                     EHAFPWLDGPVVRLCCPDTPVPFSPTLEEAYLPNVDKLVAKVRELASY"
     gene            complement(1529..2599)
                     /locus_tag="VEL74_09030"
     CDS             complement(1529..2599)
                     /locus_tag="VEL74_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HYG62712.1"
                     /translation="MAKLSSKSWGPLHPQTEQTDKLARTELPRERQLDLYRFMKLTRM
                     VEGRLGNLYRQGKVVGGLYSSLGQEATSVGSAYALDPQDVVGPLIRNLGTMLVRGVQP
                     REVMMQYMAKSDSPTGGKDANTHFGDISRGLVAPISMLGAVIPVMAGVALAGRKLGKD
                     LVAMTYIGDGGTSTGDFHEGLNLAAVLNVPFVLVAEHNGWAYSTPTSKQMRIQNIATR
                     AAAYGIPGETVDGNDVLAVYEATRRAVERARRGGGPSLIEAKTFRMKGHAEHDDAGYV
                     PAEQFEVWKKKDPIERFERHLLGNGLATEEELRAITADIDSRLNAEVDFALASPMPPP
                     ERAFEGVYAEDAGTGNGKGGVV"
     gene            complement(2611..>3297)
                     /locus_tag="VEL74_09035"
     CDS             complement(2611..>3297)
                     /locus_tag="VEL74_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYG62713.1"
                     /translation="KMTSFEKTDNGVRLKLEGGKVDSVEAEALLVAIGRKPVTEGLGL
                     EALGIELDRGYVKVNQLMQTAVPHIYAIGDVVNTPWLAHVASAEGILAVEHMAGKPVR
                     PVNYDRVPSCTYCEPEVASVGLTEAKARERGYDVAVGKFPFTALAKAAILGKTAGFVK
                     VVRDKKYDEVLGVHIVGAHATDLIAEACVALQMEGTDEELFRTMHAHPTLSEAVAEAA
                     HAAHGHAIHI"
BASE COUNT          438 a         1173 c         1133 g          553 t
ORIGIN      
        1 ccctcgctct ccttcgcctg ggacgactcg ccggaatcgc ggcgcacgga ctgctcgcgg
       61 tcggcggtgg agccggcggc cggctgggcc tcctgccgcg gagccggctg gggctccggc
      121 gacgccacct tgggcgcgct gtcggtggcc gccgcgcccg gctcgcgggc cgcctcgacc
      181 tgcttgatct cctcggtgct ctcgggcggc gccgcggccg cgaccgcctc gggctgctgc
      241 gccggacccg tggcggcggg agccgccgcg ccggcgccgg gctgctcacc ggcttcgccg
      301 atcaccgcca ccacctggtt gacggggacc gtctccccct cctggttgcg gatctccagc
      361 aggacgcccg agcccgggct gggaatctcg gcatcgacct tgtcggtcga gatctcgaac
      421 agcggctggt ccctctccac ccgatcgccc accttcacca gccacttggt gatggttccc
      481 tcggcaatcg actcccccat ctggggcatg acgacttcag ttgccatatt gctctctccc
      541 gtctccggtt cctgtcagta gctggccagc tcccgcacct tggcgacgag cttgtccacg
      601 ttgggcagat aggcctcttc gagcgtcggg ctgaacggca ccggggtgtc cgggcagcag
      661 agccggacga ccggcccgtc caaccagggg aaggcatgct cggagatgcg cgacgccacc
      721 tcgccggcga ccgacccggt caactgctcc tcggtcagga cgatgacccg gctggtcttg
      781 cggacggtgg cgaggatcgc ctcctggtcc aggggcttga tggtgcgcag gtcgaggact
      841 tcgaggtcga tcccgtcggc ttcgaggcgc ccggcggcct ccagcgccac gtgaagcata
      901 gcaccccagg tgatgaccga caggtgccgg ccctcccgcg ccaggcgcgc cttgccgatc
      961 ggcaccaggc cgtcgcccgc cggcagctcc tccttgatcc gccggtagag gtacttgtgc
     1021 tcgcagaaga gcaccgggtt ggggtcgcgg atcgccgcct tgaggagccc ccgcgcgtcc
     1081 gccggcgtcg ccggcgacac gatcttgagg cccggcgcgt ggtggaagaa ggcttccggg
     1141 ttctggctgt ggaaggccga gccgtggacg ttcccccccg aggggccgcg caccacgatc
     1201 ggcacgctct gcccccaacg gtagtggttg ggcgcggcga agttgaccat ctggttgaag
     1261 gagcaggcga tgaagtcgat gaactgcatc tccaccaccg gccgcaggcc catcatggag
     1321 gcgccgatgg cgcagcccac catggccgac tcgctgatcg gggtgtcgat gatccgctcc
     1381 gggccgaaac gctcgaggaa cccggacgtc acccggaagg cgccgccgta ggtgccgatg
     1441 tcctggccca gcatgaagac ccgctcgtcc tgctccatct cctcccagag cgcctggcgg
     1501 atggcttcga gataggtgac gacggccatc agaccacccc tcccttgccg ttgccggtgc
     1561 cggcatcctc agcgtagacc ccctcgaagg cccgctcggg cggcggcatg ggcgaggcca
     1621 gggcgaagtc gacctcggcg ttgagccggg agtcgatgtc cgccgtgatc gcccgcagct
     1681 cctcttcggt ggccaggccg ttgccgagga ggtggcgctc gaagcgctcg atggggtcct
     1741 tcttcttcca gacctcgaac tgctccgccg gcacgtagcc ggcgtcgtcg tgctcggcgt
     1801 gccccttcat gcggaaggtc ttggcttcga tgaggctcgg cccgccgccg cggcgcgccc
     1861 gctccaccgc ccggcgggtg gcctcgtaga ccgccagcac gtcgttgccg tccacggtct
     1921 ccccggggat gccgtaggcc gcggcgcggg tggcgatgtt ctggatgcgc atctgcttcg
     1981 aggtgggcgt cgagtaggcc cagccgttgt gctcggcgac gaggacgaag gggacgttca
     2041 gcacagcggc gaggttgagc ccctcgtgaa aatcccctgt ggaggtgccg ccgtccccga
     2101 tgtaggtcat ggctaccagg tccttgccca gcttcctccc cgccagggcg acgccggcca
     2161 tgaccgggat caccgcgccg agcatggaga tgggcgccac gaggccacgg gagatgtccc
     2221 cgaagtgggt gttggcgtcc ttgccgccgg tggggctgtc gctcttcgcc atgtactgca
     2281 tcatgacctc gcgcggctgg acgccgcgca cgagcatggt gccgaggttg cggatgaggg
     2341 gccccaccac gtcctgggga tccagcgcgt aggcgctgcc gacggaggtc gcctcctgcc
     2401 ccaggctcga atagagcccg cccaccacct tgccctgacg gtagaggttg cccagccggc
     2461 cctcgaccat gcgggtcagc ttcatgaacc ggtacagatc gagctgccgc tcgcgcggca
     2521 gctcggtccg ggccagcttg tcggtctgct cggtctgggg gtggagagga ccccagctct
     2581 tgctgctgag cttcgccatg gactcctttc ctagatgtgg atcgcgtggc cgtgggccgc
     2641 gtgggccgcc tcggccaccg cctccgacag agtcgggtga gcgtgcatgg tgcggaacag
     2701 ctcctcgtcg gtgccctcca tctggagcgc gacgcaggcc tcggcgatca ggtcggtggc
     2761 atgggcgccg acgatgtgga cgcccagcac ctcgtcgtac ttcttgtcgc gcaccacctt
     2821 gacgaagccg gcggtcttgc cgaggatcgc ggccttggcg agcgcggtga aggggaactt
     2881 gcccaccgcc acgtcgtagc cccgctcgcg cgccttggcc tcggtcaggc cgacgctcgc
     2941 cacctcgggc tcgcagtagg tgcaggaggg gacgcggtcg tagttgaccg gccggaccgg
     3001 cttgcccgcc atgtgctcca ccgccaggat cccctcggcc gaggcgacgt gggcgagcca
     3061 cggcgtgttg accacgtcgc cgatggcgta aatgtggggc accgccgtct gcatgagctg
     3121 gttgaccttg acgtagccgc ggtcgagctc gatccccagc gcctccagcc ccaggccctc
     3181 ggtcaccggc ttgcggccga tggccaccag cagcgcctcg gcctcgaccg agtccacctt
     3241 gcccccttcg agcttcaagc ggacaccgtt gtcggtcttc tcgaagctgg tcatctt
//
LOCUS       DASPJI010000342         6991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_828702, whole genome shotgun sequence.
ACCESSION   DASPJI010000342 DASPJI010000000
VERSION     DASPJI010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828702"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VEL74_09040"
     CDS             complement(<1..306)
                     /locus_tag="VEL74_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62714.1"
                     /translation="MPIHKRESHRVPSHLSELSRRPSAPPRDLVLVSAGLDLDGGGRA
                     LAGRLLAGACAELARSRGVGFRVLTLDGQLPPGVTAEARSFGGRQKALALRVWRDQVR
                     "
     gene            complement(802..1605)
                     /locus_tag="VEL74_09045"
     CDS             complement(802..1605)
                     /locus_tag="VEL74_09045"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HYG62715.1"
                     /translation="MGTDSNNGNGWTAEIERIHTALEQAAGLLRRYTPGDIAARRKQG
                     DDPVTEADTSVDTLLRELLPRDGEGWLSEETTDDLARLSCRRVWVVDPLDGTREFVEG
                     IPEWSVSVGLVVDGEPVAGGICNPATGQTIVGAVGAGVTLNGKPAGVSGRTQLAGARV
                     LASRSEVKRGEWQGYREGAFEMEPTGSVAYKLAQVAAGLADATWTLTPKHEWDVAAGV
                     ALVLAAGGRVITGKPEETRFNRAKPLLTRLIAATPELVPAIEEEITRRG"
     gene            complement(1608..3410)
                     /locus_tag="VEL74_09050"
     CDS             complement(1608..3410)
                     /locus_tag="VEL74_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HYG62716.1"
                     /translation="MAQVTPFPSPAPDRDRDRNPDSPASHLIEPHGGELVDLMVEPER
                     AAELKAASRDWTSWDLTPRQFCDLELLMSGAFSPLNGFMSRADHDAVCEGMRLADGTL
                     WPIPIVLDVPAEMAQKLGKSGSVLALRDPEGVMLAALHVDEVWEMDRERSAKSVFGTT
                     DLVHPGVAHLLQRSHPFAVSGRIEGLQMPIHYDFRQLRHTPAELRAEFAREGWRRVVA
                     FQTRNPMHRAHCELNLRAAKEAQANLLIHPTVGMTKPGDLDHYTRVRCYQAVINRFPQ
                     HTVKLSLLPLAMRMGGPREALLHAIIRKNYGCTHFIVGRDHAGPGKDSQGKPFYGPYD
                     AQELLRKHQEELGVTMVPFQQLSYVEDLDTYLPQDEIPEGARVLDISGTDLRRRLAEG
                     REIPSWFTFPEVAEELRRTHPPRHQQGFTVFLTGLSGAGKSTIASVLLVKFMEMGGRP
                     VTLLDGDIVRKNLSSELNFSKEHRDINIRRIGFVASEITKNGGIALCAPIAPYDSVRR
                     EVRATIEPGGGFVLVHIATPIETCEERDRKGLYAKARAGILKEFTGISDPYEVPTDAD
                     LVIDTTVMSPEEAAQEILLYLEREGYVGASMTAQ"
     gene            complement(3560..4216)
                     /locus_tag="VEL74_09055"
     CDS             complement(3560..4216)
                     /locus_tag="VEL74_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62717.1"
                     /translation="MRARALFAAACGLLLGYGATAAHAQTITVEDPGCLPREENGVVR
                     VRVNGNGHGNGAAGQAGRLYFRWKDHKAFYWVPLEMEPDGRYWAVPPKPERRNESVEL
                     YAALVDGAGNVTARSEPRQVKVNGDCRVQLSAKERGVAENLTVGETAPDQQGKEVYAF
                     LCDGVVTRVNSQGIRRSDEICRACVIAWWPRKSVLLPAAAVVGVIVSDDPEPSPSRPR
                     "
     gene            complement(4255..5202)
                     /locus_tag="VEL74_09060"
     CDS             complement(4255..5202)
                     /locus_tag="VEL74_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62718.1"
                     /translation="MRRTRIFLAAALLLAPGAARAADPFYTGMLRDGMIAYDRKDYAA
                     AARDLRVACFGMLDEPKPLADCLVRLALAQDRAGNATGFGDTFRLLLDVEERFGAYSQ
                     AELPADLKAAFEQRLVATMPAAVLESVAAFRGLAARQKPEAQGAKTTPAGRNERGRRD
                     SPPGQPAQAAKPEPVVQAPAAIAQAAPPAPRPVSEEERQKMASAREILEGDGKTRDLK
                     RAFQLAREVADAHAGSTEAQHLAAEAAYRLSRWQEAAAYFRRGGEPAADQPELLFYMA
                     VALYESGDKAAAAGALQRSLPNLQKTPYVESYAKKILGS"
     gene            complement(5199..5924)
                     /locus_tag="VEL74_09065"
     CDS             complement(5199..5924)
                     /locus_tag="VEL74_09065"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HYG62719.1"
                     /translation="MIDLHCHILPGIDDGARSLAEAVNMCRLAAEEGCEAMVATPHQR
                     RGVWWNSDLAQLAALREELQAAVGPVPRILPGGEIHVDAELLAEVERLPDAGILPLAG
                     SRYLLVEFGSNHTGAEAIHLIHELVVAGWRPIVAHPEFIPWLDLPLMERLVELGATAQ
                     VTAMSLTGDFGRRPQQDALALIDAGLVHFVASDAHGVQRRPPGLKRACLVIAGRWNEE
                     LARRLTVDNPRAVVEDRPLPEMA"
     gene            complement(5924..>6991)
                     /locus_tag="VEL74_09070"
     CDS             complement(5924..>6991)
                     /locus_tag="VEL74_09070"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HYG62720.1"
                     /translation="KALMPGGAFRPSLRQDLTYGLLMGLLLGVGCAFLIEFLDRTVKS
                     PEEIERKLGLAPLAVIQDISDAGKAYGYARNGGYGYGYGTEPPSPPTEGTRVRPKRAA
                     GTPAGWLERKKGAAPQTAQIELVPHEQPRTLISESYRSLRTALLLSSARELKLVAVTS
                     AVAGEGKTATAANLAVVLAQLGRPVLIVDCDLRKPRLHQVFRVSNRVGVVNHLTSTAD
                     PESVFLPTEVPNLWVTPSGPIPPNPSELLASDRMREWLRTVRQRFEYVIIDTPPALAV
                     TDSTIIGVLADGVVLTMRCGKVTREEARMCRDRLRLADVRILGAVLNRYRSLQAGFGK
                     RYRAYETYAADEDAAKAGSAA"
BASE COUNT          976 a         2438 c         2538 g         1039 t
ORIGIN      
        1 cctgacctgg tcgcgccaga cccgcagcgc cagcgccttc tggcgtcccc cgaaggaccg
       61 ggcctccgcc gtcaccccgg gagggagctg gccgtccaag gtgaggacgc ggaagccgac
      121 cccccgggag cgggcgagct cggcgcaggc cccggccagg agccgccccg ccagggcacg
      181 gccgccgccg tccaggtcca ggccggccga gacgagcacc aggtcacggg gtggagcgga
      241 tggccgtcgg gacagctcgg acaggtggga cgggacgcga tgagattcgc gcttgtggat
      301 cggcacgtcg ggctacagct caactccaga gatcagcttt tgaaacagaa ggcgcggggg
      361 gattgtacaa cagggtgtgc aaaccggagt cgggccttct gctccggcgg acctcgcggc
      421 gaaagctccg aagtcgcggc gcggcgttcg agcttgcggt gagccgtctg ggcttgcggt
      481 gaaccgcctg aagttgcggt gaacgctttg agcttgcggt ggatccttcg atcttgcggt
      541 gagccaagcg aagtcgcggt gaatcttttc agcttgcggc gaatctttgg aagtcgcggt
      601 gaaccgattc gagtcgcggc gagcggttcg aggtcgcggt gaagccgccg gagtcgcggc
      661 ggacggaccc gagtcgcggt gaagagtttg gagtcgcggt ggagggatcg aagtcgcggc
      721 cagcgaattg ggaacgcggc caacgaatcg gagtcgcggc gccgataccg agggaagagg
      781 gaggggggga gggacgagcc ctcacccccg ccgggtgatc tcttcttcga tcgccggcac
      841 cagctccggc gtggccgcga tcagccgggt gaggagcggc ttggcgcggt tgaagcgggt
      901 ctcttcgggc ttgccggtga tgacgcggcc ccccgcggcc aggaccagcg ccacgccggc
      961 ggccacgtcc cactcgtgct tcggggtgag ggtccaggtg gcgtcggcga gaccggccgc
     1021 cacctgcgcc agcttgtagg ccaccgagcc ggtgggctcc atctcgaagg ccccctcgcg
     1081 gtagccctgc cactcgccgc gcttcacctc gctgcggctc gccagcaccc gggcgccggc
     1141 gagctgcgtc cgcccgctca ccccggcggg cttgccgttc agggtgacgc cggcgccgac
     1201 cgcgccgacg atggtctgcc cggtggcggg gttgcagatc ccccccgcga ccggctcgcc
     1261 gtccaccacc agccccaccg acaccgacca ctcggggatc ccctcgacga actcacgcgt
     1321 gccgtcgagc gggtccacca cccagacgcg gcggcaggag agacgggcga ggtcgtccgt
     1381 cgtctcctcc gacagccacc cctcgccgtc gcgcggcagg agctcgcgca gcagcgtgtc
     1441 cactgaggtg tcggcctcgg tcacggggtc gtccccctgc ttgcgccgcg ccgcgatgtc
     1501 gcccggcgtg taacgccgga gcaggccggc ggcctgctcc agcgccgtgt ggatccgctc
     1561 gatctccgcg gtccagccgt tgccgttgtt ggagtcggtg cccatggtca ctgcgccgtc
     1621 atgctcgccc cgacgtagcc ctcccgctcc agatagagca ggatctcctg ggccgcctcc
     1681 tcgggggaca tgaccgtggt gtcgatcacc aggtcggcgt cggtgggcac ctcgtacggg
     1741 tcggagatac cggtgaactc cttgaggatg ccggcgcgcg ccttggcgta cagccccttg
     1801 cggtcccgct cctcgcaggt ctcgatcggc gtcgcgatgt ggaccagcac gaagccgccg
     1861 cccggctcga tcgtcgcccg cacctcccgc cgcacgctgt catagggggc gatcggggcg
     1921 cacagggcga tgccgccgtt cttggtgatc tcggaggcga cgaagccgat gcgccggatg
     1981 ttgatgtccc ggtgctcctt cgagaagttg agctcggacg acaggttctt gcgcacgatg
     2041 tcgccgtcga gcagtgtcac cggccggccg cccatctcca tgaacttcac cagcagcacc
     2101 gaggcgatgg tcgacttgcc ggcgcccgag aggccggtga ggaacaccgt gaagccctgc
     2161 tggtgccgcg gcggatgggt gcggcgcagc tcctccgcca cctcggggaa ggtgaaccag
     2221 gacgggatct cccggccctc ggccaggcgc cggcgcaggt ccgttcccga gatgtccagc
     2281 acgcgcgccc cctcggggat ctcgtcctgc ggcaggtagg tgtccaggtc ctcgacgtag
     2341 ctgagctgct ggaacggcac catcgtcacc cccagctcct cctggtgctt gcgcagaagc
     2401 tcctgagcgt cgtaggggcc gtagaacggc ttgccctgcg agtccttgcc cgggccggcg
     2461 tggtcgcggc cgacgatgaa gtgggtgcag ccatagttct tgcggatgat ggcgtgcagc
     2521 agcgcctccc gcgggccgcc catgcgcatg gcgagcggga gcagcgacag cttcaccgtg
     2581 tgctgcggga agcggttgat caccgcctgg tagcagcgca cgcgggtgta gtggtcgagg
     2641 tcgcccggct tggtcatgcc caccgtcggg tggatcagca ggttggcctg cgcctccttg
     2701 gccgcccgca ggttcagctc gcagtgcgcc cggtgcatcg ggttgcgcgt ctggaaggcc
     2761 accacccggc gccagccctc gcgggcgaac tccgcgcgca gctcggccgg cgtgtggcgg
     2821 agctgccgga agtcgtagtg gatcggcatc tgcagccctt cgatacggcc ggagacggcg
     2881 aacggatgac tgcgctggag caggtgggcc actccgggat gaaccaggtc ggtggtgccg
     2941 aacacggact tggcgctccg ctcgcggtcc atctcccaga cctcgtccac gtgcagcgcc
     3001 gcgagcatca ccccttcggg gtcgcgcagc gccaggacgc tcccgctctt gccgagcttc
     3061 tgggccatct ccgccggcac gtcgagcacg atcgggatcg gccagagggt gccgtcggcc
     3121 aggcgcatcc cctcgcagac cgcgtcgtgg tcggcgcggc tcatgaagcc gttcagcggc
     3181 gagaaggcgc cggacatcag cagctccagg tcgcagaact ggcgtggcgt cagatcccag
     3241 gacgtccagt cgcgggacgc cgccttgagc tcggcggcgc gctccggctc gaccatcagg
     3301 tcgacgagct cgccgccatg gggctcgatg aggtggctcg cggggctatc gggattccgg
     3361 tcgcggtcgc gatccggagc ggggctggga aacggagtga cttgtgccat tatagacaat
     3421 gctcctcatg cgggcgttga gactcggata cacaggtgcg gggaaacccc aaaccgggac
     3481 acacgctccc cctccgggag cgcccggtgg gtttattcaa gaatagggcc agccggtctg
     3541 ccggggcgag gaggggaggt cagcggggac gggatggaga gggctccgga tcgtcggaga
     3601 cgatcacgcc caccaccgcg gcggcgggga ggaggaccga cttgcggggc caccaggcga
     3661 tgacgcaggc gcggcagatc tcgtccgacc ggcggatccc ctgggagttg acgcgggtga
     3721 cgacgccgtc gcagaggaag gcgtagacct ccttgccctg ctggtccggc gcggtctcgc
     3781 ccaccgtgag gttctcggcc acgccgcgct ccttggcgga gagctgcacc cggcagtcgc
     3841 cgttcacctt cacctggcgg ggctcggagc gggcggtcac gttgccggcg ccgtcgacca
     3901 gggcggcata gagctccacc gactcgttgc gccgctccgg cttcggcggc acggcccagt
     3961 agcggccgtc cggctccatt tccagcggca cccagtagaa ggccttgtgg tccttccaac
     4021 ggaagtagag ccgccccgcc tgaccggccg cgccatttcc gtggccgttg ccgttcaccc
     4081 gcacccggac gacaccgttc tcctcccgcg gcaggcagcc ggggtcctcc accgtgatcg
     4141 tctgggcgtg cgccgcggtg gcgccgtatc ccagaagcag gccgcaggcc gcggcgaaga
     4201 gggctcgtgc tctcatggtt ttctcccgtt tggccgattt tacctcactc ggcctcagct
     4261 ccccagaatc ttcttcgcgt acgactccac gtagggcgtt ttctgcaaat tcggcagcga
     4321 gcgctggagg gcgccggccg cggcggcctt gtccccggac tcgtaaagag cgaccgccat
     4381 gtagaacagc agctccggct ggtccgccgc cggctcgccg ccgcgccgga agtaggcggc
     4441 cgcctcctgc cagcgggaga gacgataggc cgcctcggcc gccaggtgct gcgcctccgt
     4501 cgacccggca tgggcgtcgg ccacctcgcg ggcgagctgg aaggcgcgct tgaggtcccg
     4561 ggtcttgccg tcgccctcga ggatctcgcg cgccgaagcc atcttctgcc gttcttcctc
     4621 ggagaccggc cggggagccg ggggcgccgc ctgggcgatg gccgcggggg cctggaccac
     4681 cggctccggc ttggcagcct gcgccggttg ccccggcgga gaatctcgcc gtccccgctc
     4741 gttcctgccg gcgggcgtgg tcttcgcccc ctgggcctcc ggcttctggc gggcggcgag
     4801 gccgcggaag gcggcgacgc tctccaggac cgccgccggc atggtggcca ccaggcgctg
     4861 ctcgaaagcg gctttcaggt ccgccggcag ctcggcctgg gagtaggcgc cgaaccgctc
     4921 ctccacatcc aacaggagac ggaacgtgtc gccgaagccg gtggcgttgc cggctctgtc
     4981 ctgcgccagg gccaggcgca ccaggcagtc ggctagcggc tttggctcgt ccagcatgcc
     5041 gaagcaggcg acgcgcaggt cgcgtgccgc cgccgcgtag tccttgcggt cgtaggcgat
     5101 catgccgtcg cgcagcatgc cggtgtagaa cggatcggcc gcgcgggcgg ctccgggagc
     5161 cagcagcagg gcggcggcga ggaagatacg ggtacgtctc atgccatctc cggcagcggg
     5221 cggtcctcga cgaccgccct cgggttgtcc acggtcaggc gcctggccag ctcctcgttc
     5281 catctcccgg cgatgaccag gcaggcccgc ttgaggcccg gggggcggcg ctggacgccg
     5341 tgggcgtcgc tggccacgaa gtggacgagc ccggcgtcga tcagcgccag cgcgtcctgc
     5401 tgcgggcggc gcccgaagtc gccggtgagg ctcatcgccg tcacctgggc cgtcgccccg
     5461 agctcgacca gccgctccat cagcggcagg tccagccagg ggatgaattc ggggtgcgcc
     5521 acgatggggc gccagccggc caccaccagc tcgtggatca ggtggatcgc ctcggcgccg
     5581 gtatggttgg agccgaactc gaccagcagg tagcgcgagc cggccagggg gaggatgccg
     5641 gcgtccggca gccgctccac ctcggcgagg agctccgcgt cgacgtggat ctcacccccg
     5701 ggcaggatgc gcggcaccgg ccccaccgcc gcctgcagct cctcgcgtag cgcggcgagc
     5761 tgggcgaggt cggagttcca ccagacgccg cgccgctggt gcggggtggc gaccatggcc
     5821 tcgcacccct cctccgcggc gaggcggcac atgttgaccg cctcggccag gctgcgggca
     5881 ccgtcgtcga tgcccggcag gatgtggcaa tgcagatcga tcatcaggcg gccgatccgg
     5941 ccttggccgc gtcctcgtcg gcggcgtacg tctcataggc gcggtagcgc ttgccgaagc
     6001 cggcctggag cgagcggtag cggttgagca ccgcgcccag gatccgcacg tcggcgaggc
     6061 ggagccggtc gcggcacatc cgcgcctcct cgcgcgtcac cttgccgcag cgcatcgtca
     6121 gcaccacacc gtcggccagc acgccgatga tcgtcgagtc ggtgaccgcg agcgccggcg
     6181 gcgtgtcgat gatcacgtac tcgaagcgct ggcgcaccgt ccgcagccac tcccgcatcc
     6241 ggtccgaagc cagcagctcc gacgggttgg gcgggatcgg ccccgacggc gtcacccaga
     6301 ggttgggcac ctcggtgggc aggaagaccg attccgggtc ggccgtggag gtcaggtggt
     6361 tgacgacgcc gacccggttc gacacccgga acacctggtg caggcggggc ttgcgcaggt
     6421 cgcagtcgac gatcagcacc ggccggccga gctgggccag gaccacggcg aggttggcgg
     6481 cggtggcggt cttgccctcg ccggcgaccg ccgaggtcac cgccaccagc ttgagctcgc
     6541 gggcggacga gaggaggagc gccgtacgca gcgaccggta ggactcggag atcagcgtcc
     6601 gcggctgctc gtgcggcacg agctcgatct gcgccgtctg cggcgcggcc cccttcttgc
     6661 gctccagcca ccctgccggc gtgcccgccg cccgcttggg gcgtacccgc gtcccctcgg
     6721 tgggggggga cggcggctcg gtgccatagc cgtagccata gccgccgttg cgcgcgtagc
     6781 cgtaggcctt gccggcgtcg gagatgtcct ggatcaccgc cagcggggcg agccccagct
     6841 tgcgctcgat ctcctccggc gacttgaccg tgcggtccag gaactcgatc aggaaggcgc
     6901 agccgacgcc gaggagcagg cccatcagca ggccgtaggt gagatcctgg cgcagcgacg
     6961 gacggaacgc gccgccgggc atcagcgcct t
//
LOCUS       DASPJI010000343        10640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_828781, whole genome shotgun sequence.
ACCESSION   DASPJI010000343 DASPJI010000000
VERSION     DASPJI010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10640)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10640
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828781"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..10210)
                     /locus_tag="VEL74_09075"
     CDS             complement(<1..10210)
                     /locus_tag="VEL74_09075"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62721.1"
                     /translation="MKSLVELLHRQATEQPGRGYTFLTQGEEAGARLSYDGLQRRART
                     IAAVLARSVPRGERVLLLYPSGVELIAAFFGCLYAGVIAVPVQPPHGRRPDPRLGGVA
                     ADCRPRAVLTTAALLVRREGIAGQVPELASALWLDTGTVDEDEDAGREPGPEEVAFLQ
                     YTSGSTGTPKGVVVTHANLLHNLEAIRRAFRQTAESVVVGWLPLFHDMGLIGNVLQPC
                     YVGCDCVLMPPAAFLQRPARWLRAIERFGGTTSGGPNFAYDLCVRALGPEEREGLDLS
                     SWSVAFNGAEPVRAETLRRFAEAFAPCGFRREAFFPCYGLAEATLFVAGGPPDGEPGI
                     GTFASGPLEAGTAVPAGDGAGRELVSCGRASAEQRIVVVDADSGVPCPEGRVGEVWVA
                     GPSVAAGYWGRPDESAATFRARLGGEAFLRTGDLGFLAGGELFLTGRLKDLIILRGRN
                     LYPQDLELTAERSHPALRAGGAAFSVDGQGEERVVLALEVERHAGDLGAVADAVRRAV
                     ADEHGVAVADVLLLRGGTVPKTSSGKIRRRECRARYLAGSLAPLHRSAPPGAAPGAGL
                     LTRFDLLALDPPERSAALTAWLRGEIARRAGVATEALDPAAPIAAAGLDSLALFELQA
                     RLESDLGLAPAAASLADLSVPELRDRLLDHLTEVSESTPLVTGEAAGDHPLAPGQEAL
                     WFLESSTAADGVFHIAAAARLGPEVDGAALFRAAQALAERHPALRTTFGLWDGEPRQR
                     VHATLPPDLGEVGATAWSAAELEAGLRREVRRRFDLERESPLRLRVLTRPGGERLLLL
                     VLHHLAGDFGSLAVLLHDWAALYARERDAGRPPLPPLLPLSGAYTDFVRWQRRLLAGP
                     AGERLERYWLDRLRGAPLALDLPADRPRPPSPAHAGARLGLRLGSGETGRLRERARAH
                     GATLFTALLATFQILLGRLTGQDDLLVGSPATGRRDRDLDGVVGYFVNPMVLRAELAG
                     DPPVPELLARSRRAVSEALAHRDFPFPWLTRRLQPVRDPARPPVFQVQLVFQRAAPGQ
                     EPGLAAFAVGEEGARVELEGLALESLRLDPGTSQLDLTLFAAEAGDALALACEYDTAL
                     FDGVTVARWLGHLRTLLAAAAAWPERRIGELPLLGDAERHQLLREWSDTAVAREGAGA
                     CLHHLFEEQARRTPDAVALIAGGREVRYHELERDAARLARRLRREGAGPEVVIGVCLG
                     RSAALVTALLGVLKAGAAYLPLDPQLPRPRLAALLAESRASLVVADRERAAGLPASGS
                     LILVDQILEDEDGGDERPAEEPGAEPANLAYVLYTSGSTGAPKGVAVTHRSAVELVRW
                     AATVYAPEELAGVLAATSAAFDLSVFEIFVPLSWGGTVILARNALELPDLLASAPAAG
                     RVTLVNTVPSAMAELVRTGGLGPSVRTVNLAGEPLPRALADRIYTTGTVERVWNLYGP
                     TEDTTYSTFARVAPEGTAAPTIGRPVASTRALVSSGGVEPLPVGVAGELCLGGAGLAR
                     GYLHRPDWTAERFVPDPFAAEPGARLYRTGDLARWTTAGELDFLGRVDHQVKVRGFRI
                     EPGEIEAALHEYPGVHQAVVVARRDGPAGPARLVAYTVAAPASAPAAAELRSFLAERL
                     PAYMVPAAWVALAALPLTPNGKVDRAALPVPDGALPSREHVAPRTRAEEILAGLWAEV
                     LRLPRVGVHDNFFELGGDSILSLQIVARARRAGLLLSTRQVMEHQTVAELASLCSDEA
                     AAGPGAAGSAEQGSVVGEVPLTPIQRWFFAQRFADLHHFNQALLLEPAEPLVPTALER
                     ALAAVVEHHDALRLRFHRLRRGGEGGWRQENAAAEPVPPFHRIDLSGLPAACRPEARE
                     RAVALLQTGFDLAAGPLTRLALFDTGELLWAAHHLVVDAVSWRVLLEDLEDAYRQAAR
                     GLRPTLPPKTSSFRDWAVRLAAHAVQGAPAHELEHWLAVARTAVPPLPVDLQGGSDVA
                     GAEDAVSFELTEEETGGLLQAVPAVCHSRVDEALLSALVRSLAAWTGSPRLRVDVEGH
                     GRDPLFDDLDLSRTVGWFTSIHPVILEAGGGGPGEALVSVKERLRAVPGRGLGYGLLR
                     YLGEKETASLLAAAPAAEISFNYLGRLDSGESSLFRPSLAPAASTRSPRALRPHRLEV
                     GALVTGGRLRVTLAYGSRVLRRETAERLAAAFAGALDELIRHGRACERQVFTPSDFPK
                     AGLDAGRFARLAADLPLEEVEDIYPLSPLQEGILLHSQLASASGVYVNQIACTLPADL
                     EPGLFRQAWEILVARHGALRTAFLWAGLDEPLQAVHRRVPLPWQELDWRGLPGDEQRR
                     RFEELLHRDRHTPLSLKASHGTVPPLRLSLVHLDGALGFVWTFHHLLLDGWSVQVLVG
                     ELGSVYGALREGRTPALPPIRPFSDFIVWLRRQDPARAEPVWRRELAGFTAPTSLSLA
                     RPAGAGVSGEAEHTAGLAPAVTADLQSLAARHRLTLQTVTLGAWAVLLGRYGGEEDVV
                     FGSVVSGRPAALPGVESMVGLFINSVPVRARVKGGEALPAWLRDLQERRLALADGEHA
                     PLSLIQGWSEVPSGAPLFETLHVFENYPAADLGGLRAADLRSFETTHYPVTLAVTAGD
                     RLSLRLTVDRARVEETAAPRLIHHLGVLLAGMAEAPERRLGEIPLLSAGERQQLQLEW
                     NDAALPAAPGVVELFTAQAHRSPEAMAVVPAPAGDGGEGLTYAELDRRSDRLAGRLRS
                     LGVGPEVPVGLCVEHTPELAVAVLGIFKSGGALLALDPAHPPARLSLLLEDAAVPVLV
                     TQPHLQKGLPPHGARVVLLDGEEDGDEDGRESAAPPAPDSLAYLIYTSGTTGRPKAVQ
                     VEHGTLAATLAATRERFGFAAGERMPCLALPTFDIFLFELLTPLLTGGAAVLLPLRPV
                     LDVERLVDRLGELTCLHAVPALMRQIVDALRRRSAAAADVGGVRHVFVGGDVVPSELL
                     EELRETFPAARVWVLYGPTEATIVCAAHPVPPPPAPARSLLGRPLGGTVLHVCAPDGG
                     LLPPGVAGELWIGGAGVARGYRGRDELTAERYVRRGGDRFYRSGDRVRRLPDGTLEFL
                     GRLDQQVKVRGIRVEPGEIEAALAAQPGVREAVVVLREDTAGERRLVAYVAGDAAPEE
                     LRRALRERLPQALVPAAFVRLAALPLTPNRKVDRQALPAPERQGDGEDHRAPRTPVEE
                     VLAGIWAELLGRERIGADDHFFDLGGHSLLATRVMSRLWSAFGIELPLTAIFEAPVLA
                     DLAARVETALRAGTANRRPPLLPRAPGSRDAALPLSFAQQRLWFLDRLEPGRPTYNMP
                     AALRVEGTLSAGVLARCLEEIESRHEALRTVFAELEGAPVQVVRPPAPFRLPVVDLSG
                     LPDREAAALALAGEEATRPFGLARGPLLRCLLLRLGEGDHVLALTLHHIAGDG"
     gene            10386..>10640
                     /locus_tag="VEL74_09080"
     CDS             10386..>10640
                     /locus_tag="VEL74_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HYG62722.1"
                     /translation="MDERQQSGRPLAASGRDARVIAMVVLALLGAWISFHLTRYHLAG
                     SGHGGSGVFHTVCEMTGGGCDQILKSTWSTLPRQIPTAFAG"
BASE COUNT         1519 a         3968 c         3997 g         1156 t
ORIGIN      
        1 agccgtcgcc ggcgatgtgg tgcagggtga gggcgaggac gtggtccccc tcccccaacc
       61 gcagcaggag gcagcgcagc agcggaccgc gggcgaggcc gaacggccgg gtggcctcct
      121 ccccagccag ggccagggcc gccgcctcgc ggtccggcag ccccgaaagg tccaccacgg
      181 gcaaccggaa cggcgccggc ggccggacca cctgcaccgg cgctccctcc aactcggcga
      241 aaaccgtgcg cagggcctcg tgacggctct cgatctcctc caggcagcgc gccagcacgc
      301 cggcgctcaa ggtgccctcg acgcgtaggg ccgccggcat gttgtaggtc ggccggccag
      361 gctcgaggcg gtcgaggaac cagaggcgct gctgggcgaa cgacaggggc agggcggcgt
      421 ctcgcgatcc cggagcgcgg gggaggagag gcggtctgcg gttcgctgtg ccggcgcgta
      481 gcgccgtctc gacccgcgcg gcgagatccg ccagcaccgg ggcctcgaag atggcggtca
      541 gcggcagctc gatgccgaac gcgctccaca ggcgcgacat cacacgggtg gccagcaacg
      601 agtggccgcc cagatcgaag aaatggtcgt ccgctccgat ccgctcacgg ccgagcaact
      661 cggcccagat gccggcgagg acctcctcga caggcgtccg cggcgcgcga tggtcttcgc
      721 catcaccctg gcgttccgga gccggcaggg cctggcggtc caccttacgg ttgggcgtca
      781 gggggagcgc cgcgaggcgg acgaaggcgg ccggcaccag ggcctgtggc agccgctccc
      841 gcaacgccct gcgcagctcc tcgggagcgg cgtcgccggc cacgtaggcc accagccgcc
      901 gctctccggc ggtgtcctcg cgcaagacca ccaccgcctc gcgcactccg ggctgcgcgg
      961 cgagcgccgc ctcgatctcc cccggctcca cgcggatgcc gcgcaccttg acctgctgat
     1021 cgagacggcc gaggaactcg agggtgccgt cgggcagcct ccgcacgcgg tcgccgctgc
     1081 ggtagaatcg atcgccgcca cgccgcacgt acctctccgc ggtcagctcg tcccgccccc
     1141 tgtagccgcg ggcgaccccg gcaccgccga tccacagctc gcccgcaacg ccgggcggca
     1201 gcagcccgcc gtccggcgcg cagacatgaa gcacggtgcc cccgagcggc cggccgagca
     1261 gcgatcgcgc cggcgcgggc ggcggcggca ccgggtgcgc ggcgcagacg atggtcgctt
     1321 cggtgggacc gtagagcacc cagacccggg cggccgggaa ggtctcccgc agctcctcca
     1381 gcagctcgga cggcaccacg tcgccgccga caaagacgtg gcgcaccccc ccgacgtccg
     1441 ccgccgcggc gctgcggcgg cggagcgcgt cgacgatctg gcgcatcagc gccgggaccg
     1501 cgtgcaggca ggtcagctcc cccaggcggt cgaccagccg ctccacatcg agcaccggcc
     1561 gaagagggag gagcacggcg gcgccgccgg tgagcagagg ggtcaacagc tcgaagagga
     1621 agatgtcgaa cgtggggagc gccaggcaag gcatgcgctc gccggccgcg aaaccgaacc
     1681 gctcccgcgt cgccgcgagg gtggccgcga gcgtgccgtg ctcgacctgg acggctttgg
     1741 gccggcccgt ggtgccggag gtgtagatca ggtaggcgag gctgtccgga gccggagggg
     1801 cggcactctc tcgcccatct tcatccccat cctcctctcc atccagcagg acgaccctcg
     1861 cgccgtgcgg cggcaggccc ttctggagat gcggctgagt caccaggacc gggaccgccg
     1921 cgtcctccag cagcagggac agccgtgccg gcggatgggc ggggtccagc gccagcaggg
     1981 cgccgccgga cttgaagatc cccagcaccg ccacagccag ctccggcgtg tgctcgacgc
     2041 acaggcccac cggcacctcc ggcccgactc ccagcgagcg caggcgcccg gcgagacggt
     2101 ccgaccggcg gtcgagctcg gcataggtga ggccctcccc cccgtctccg gcgggggcgg
     2161 gtaccacggc catcgcctcc ggcgagcggt gcgcctgcgc cgtgaagagc tccaccactc
     2221 ccggcgccgc cggcagtgcc gcgtcgttcc actcgagttg tagctgctgg cgctcgccgg
     2281 ccgagagcag cgggatctcg ccgaggcggc gctccggcgc ttcggccatc ccggcgagca
     2341 gcacgcccag gtggtggatc aggcgtggcg ccgcagtctc ctcgacgcgg gcgcggtcga
     2401 cggtcaaccg cagcgagagc cggtctccgg ccgtgaccgc cagggtcacc gggtaatggg
     2461 tggtctcgaa gctgcgcagg tcggcggcgc gcagacctcc gagatcggcg gcggggtagt
     2521 tctcgaagac gtggagcgtc tcgaacagcg gcgcgccgga gggcacctcg ctccaaccct
     2581 ggatcagcga gagcggagcg tgctcgccgt cggccagggc gagacggcgc tcctggagat
     2641 ccctcagcca ggcgggcaga gcctcgccgc ccttcacccg cgcccgcacc ggcacggagt
     2701 tgatgaacag gccgaccatg gactcgacgc caggcagggc ggcagggcga ccggagacca
     2761 cgctgccgaa gacgacgtcc tcctccccgc cgtagcggcc gaggagcacc gcccaggcgc
     2821 cgagggtgac ggtctgcaag gtgagccggt gacgcgccgc caggctctgg aggtccgccg
     2881 tgaccgcggg cgcgagcccg gccgtgtgct cggcctcccc gctgactccg gccccggcag
     2941 ggcgcgcgag actcaaggag gtcggggcgg tgaagcccgc cagctcccgc cgccagacgg
     3001 gctcggcacg tgccgggtcc tgccgccgca gccagacgat gaagtcgctg aacggccgga
     3061 tcggtggcag cgcgggtgtc cggccctccc ggagggcgcc gtagacggac cccagttcgc
     3121 ccaccaggac ctgcaccgac cagccgtcca gcagcaggtg gtggaaggtc cagacgaacc
     3181 ccagcgcccc gtcgaggtgg accagggaga gccgcagcgg cggcacggtg ccatgggagg
     3241 ccttcaggga taacggggtg tgccggtcgc ggtgaagcag ctcttcgaag cggcgccgct
     3301 gctcgtcccc cgggaggccg cgccagtcga gctcctgcca ggggagcggg acccggcggt
     3361 ggacggcctg caggggctcg tccagccccg cccaaaggaa ggcggtgcgc aacgcgccgt
     3421 ggcgcgccac caggatctcc caggcctgcc ggaacagccc gggctccagg tccgccggca
     3481 gggtgcaggc gatctggttg acgtagacgc cggacgccga cgccagctgg ctgtggagca
     3541 ggatgccttc ctggagcggt gaaagggggt agatgtcctc gacctcctcc agagggaggt
     3601 cggccgccag ccgggcgaag cggccggcgt cgagcccggc cttggggaag tcggacgggg
     3661 tgaagacctg cctctcacag gcccggccgt gccggatcag ctcgtcgaga gcgccggcaa
     3721 aggccgcggc cagacgctct gcggtctccc ggcggagcac ccgcgagccg taggccagag
     3781 tgacgcggag ccggccgccg gtcaccaggg cgccgacctc gagccggtgc gggcgaagag
     3841 cgcgcgggct gcgggtggag gccgccggcg ccagggaggg ccggaacagc gaagattcgc
     3901 cggagtcgag ccggcccagg tagttgaagc tgatctcggc ggcgggggcc gcggccagca
     3961 aggaggccgt ctccttttcc cccagatacc gcagcagacc gtagccgagg ccccgcccgg
     4021 gaaccgcgcg caaccgctcc ttgacgctga ccagggcctc gccgggaccg ccgccccccg
     4081 cctcgaggat caccggatgg atgctggtga accagcccac ggtgcgcgag aggtcgagat
     4141 cgtcgaaaag gggatcgcgg ccgtgccctt cgacgtccac ccgcagccgc ggcgagccgg
     4201 tccaggccgc cagcgagcgg accagggcgc tgagcagcgc ctcgtcgacg cggctgtggc
     4261 agacggcggg cacggcctgc agcagaccgc cggtctcctc ctccgtcagc tcgaaggaga
     4321 ccgcgtcctc ggctccggcg acgtccgaac cgccttgcag gtccaccggc agcgggggaa
     4381 ccgccgtccg ggccacggcg agccagtgct ccagctcgtg ggccggcgcg ccctgcacgg
     4441 cgtgggcggc gaggcggacg gcccagtcgc ggaaggagct cgtcttcggc ggcagggtgg
     4501 ggcggagccc gcgggccgcc tggcgatagg cgtcttccag gtcctcgagc agcacgcgcc
     4561 aggagacggc atccaccacc aggtgatggg cggcccacaa cagctcacca gtgtcgaaca
     4621 gggcgaggcg ggtgagcggc ccggcggcca ggtcgaagcc tgtctggagg agcgcgaccg
     4681 cccgctcgcg cgcctcgggc cggcacgccg ccggcaggcc cgagaggtcg atgcgatgga
     4741 agggggggac aggctcggcc gcggcatttt cctgccgcca gccgccctcc ccccctcgtc
     4801 ggagacggtg gaaccgcagg cgcagggcgt cgtggtgctc caccacggcg gccagggcgc
     4861 gctccagggc ggtggggacg aggggctccg ccggctccag caggagcgcc tggttgaagt
     4921 ggtggaggtc cgcgaagcgc tgcgcgaaga accagcgctg gatcggggtg agagggacct
     4981 cgcccaccac ggacccctgc tcggcagagc cggcggcgcc aggaccggcc gcggcctcat
     5041 ccgagcagag gctggcgagc tcggccacgg tctggtgctc catgacctgc cgcgtgctga
     5101 ggagcaggcc ggcccggcgc gcccgcgcca cgatctggag ggagaggatc gaatcgccgc
     5161 cgagctcgaa gaagttgtcg tgcactccga cccgcggcaa ccgcaggacc tcagcccaga
     5221 gaccggccag gatctcctcg gcgcgggtgc gcggcgccac gtgctcgcgc gacggcagcg
     5281 cgccgtcggg caccggcagg gcggcgcggt ccaccttgcc gttgggggtg agcggcagcg
     5341 ctgccagcgc cacccaggcc gccggcacca tgtaggcagg aagccgttcc gccaggaagc
     5401 tccgcagctc ggcggcggcc ggtgcggagg ccggcgcagc gacggtgtag gccaccagcc
     5461 gcgccggccc cgccggaccg tcgcgccggg ccacgaccac cgcctggtgc accccggggt
     5521 actcgtggag cgccgcttcg atctcccccg gctcgatgcg gaagccgcgg accttcacct
     5581 ggtggtcgac gcgtccgagg aagtcgagct ctccggcggt ggtccaccgc gccaggtcgc
     5641 cggtgcggta gaggcgggcg ccgggctcgg ccgcgaacgg atccggcacg aagcgctcgg
     5701 ccgtccagtc cggacggtgc aggtaccccc gggccagccc cgcgcctccc aggcacagct
     5761 ctcccgccac acccaccggc agcggctcca ccccacccga cgagaccagg gcccgggtgg
     5821 acgcgaccgg ccggccgatg gtgggcgccg cggtcccttc cggagccacc cgcgcgaagg
     5881 tcgagtacgt cgtgtcctcg gtcgggccgt acaggttcca cacccgctcc accgtcccgg
     5941 tggtgtagat ccggtcggcg agggcgcgcg gcagaggctc tccggcgagg ttgacggtgc
     6001 gcaccgacgg gccgagcccc cccgttcgca ccagctcggc catggcggag gggacggtgt
     6061 tcaccagcgt cacccgcccg gccgccggcg cggaggcgag gaggtccggc agctccaggg
     6121 cgttccgggc caggatcacc gtgccccccc acgacagggg gacgaagatc tcgaagaccg
     6181 acaggtcgaa ggccgccgag gtggcggcga gcacgccggc cagctcctcc ggcgcgtaca
     6241 ccgtggccgc ccagcgcacc agctcgacgg cgctgcggtg ggtcaccgcc acccccttgg
     6301 gggcccccgt cgaaccggag gtgtagagca cgtaggccag gttcgccggc tcggcgcccg
     6361 gctcctccgc cggccgctcg tctccaccgt cctcatcctc caggatctga tccaccagga
     6421 tcagcgaccc gctcgccggg agcccggcgg cgcgttcccg gtcggcgacc accagggagg
     6481 cccgggactc ggcgagcagg gcggcgaggc gcgggcgcgg cagttggggg tcgagcggca
     6541 ggtaggccgc tcccgccttc agcacgccca gcagagcggt caccagggcg gccgagcgcc
     6601 ccaggcagac gccgatcacc acctccggcc cggcgccctc ccggcgcagc cggcgcgcca
     6661 gccgcgcggc gtcccgttcg agctcgtggt agcggacctc ccgaccgccg gcgatcaacg
     6721 ccaccgcgtc cggcgtccgc cgggcctgct cctcgaacag gtggtgcagg caggcgccgg
     6781 ccccctcccg cgccaccgcg gtgtcgctcc actcgcggag gagctggtgc cgctccgcgt
     6841 cgccgaggag ggggagctcg ccgatccgcc tctccggcca ggccgccgcg gcggccagca
     6901 gggtccgcag gtgccccagc cagcgcgcca ccgtcacccc gtcgaacagg gcggtgtcgt
     6961 actcgcacgc gagcgccagg gcgtccccgg cctcggcggc gaacagcgtc aggtcgagct
     7021 gggaggtgcc ggggtccagc cggagcgatt ccagcgcgag cccctccaac tcgacgcgcg
     7081 ccccctcctc tcccactgca aaggcggcca ggcccggctc ctggcccgga gcggcgcgct
     7141 ggaagacgag ctgcacctgg aagaccggcg gccgggcggg atcacgcacc ggctgcagac
     7201 gccgggtgag ccaggggaac gggaaatcgc ggtgcgccag ggcctcgctc accgcccggc
     7261 gggagcgcgc caggagctcc gggaccgggg gatcgccggc cagctcggcg cgcaggacca
     7321 tcgggttgac gaaatacccg acgacgccgt cgagatcgcg gtcgcgccgt ccggtggcgg
     7381 gagagccgac caggaggtcg tcctggcccg tgaggcgtcc gaggaggatc tggaaggtgg
     7441 cgaggagagc cgtgaacagg gtcgcgccgt gcgcccgggc gcgctcgcgc agacgtccgg
     7501 tctcgccgga gccgagccgc aggccgagcc gcgcgcccgc gtgggccggg ctcggcgggc
     7561 gcggccggtc ggccggcagg tcgagggcga gcggcgcgcc gcgtaggcgg tcgagccagt
     7621 atcgctccag gcgctcgccg gcggggccgg ccagaagccg tcgctgccag cggacgaaat
     7681 cggtgtaggc cccggacagc ggcagcagcg gcggcagcgg cggccggccg gcgtcccgtt
     7741 cccgggcgta cagggcggcc cagtcgtgca gcagcaccgc cagcgagccg aagtctcccg
     7801 cgaggtgatg gaggaccagc agcagcagcc gctcgccgcc ggggcgggtc aagacccgga
     7861 ggcgcagcgg cgactccctc tcgaggtcga accgccgccg gacctcgcgc cgcaggccgg
     7921 cctcgagctc cgccgcactc caggcggtcg cgcccacctc tcccagatcg ggcggcaggg
     7981 tggcgtggac ccgctgacgg ggctcgccgt cccagaggcc gaaggtggtg cgcaacgccg
     8041 ggtggcgctc cgcgagcgcc tgcgcggcgc ggaagagagc ggcgccgtcg acctccggcc
     8101 cgagccgggc ggcggcggcg atgtggaaga ccccgtccgc cgccgtggag ctctccagga
     8161 accagagcgc ctcctgcccc ggcgccagcg ggtggtcgcc ggcggcctct ccggtgacca
     8221 gcggcgtgct ttccgagacc tccgtcaggt gatcgagcag gcggtcccgc agctccggca
     8281 ccgagagatc ggccagggag gcggccgccg gggcgaggcc gaggtccgat tccagccgcg
     8341 cctgcagctc gaagagagca agagagtcca ggcccgcggc cgcgatcggc gccgccggat
     8401 cgagagcctc cgtggcgacc ccggcgcggc gggcgatctc cccgcgcagc caggcggtga
     8461 gcgcggcgct ccgctccggc ggatcgaggg cgagcaggtc gaagcgggtg aggagccccg
     8521 cgcccggcgc ggcgcccggc ggcgcgctgc ggtggagcgg cgccaggctg ccggcgagat
     8581 agcgggcgcg gcactcgcgg cggcggatct tgccgctcga cgtcttgggg accgtccccc
     8641 cgcggagcag cagcacgtcc gcaacagcca ccccgtgctc gtcggcgacg gcgcggcgga
     8701 ccgcgtcggc gaccgcgccg agatcgccgg cgtggcgctc cacctcgagc gccaggacga
     8761 cccgctcctc cccctgcccg tcgacggaga aggccgcgcc gccggcgcgc agggccgggt
     8821 ggctgcgctc cgcggtcagc tcgaggtcct gcgggtaaag gttgcgcccg cgcaggatga
     8881 tcaggtcctt gagccggccg gtgaggaaca gctccccccc ggccaggaaa cccaggtcgc
     8941 cggtgcgcag gaaggcctcg ccccccagcc gggcgcggaa ggtggccgcg ctctcgtccg
     9001 gccggcccca gtagccggcg gcgacgctcg ggcccgccac ccagacctcc cccacccgcc
     9061 cctcggggca gggcacgccc gaatccgcat cgacgaccac gattcgctgc tccgcggagg
     9121 cgcggccgca ggagaccagc tcccgcccgg cgccgtcccc cgccggcacc gccgtgccgg
     9181 cttccagggg gccgctcgcg aaggtgccga tccccggctc cccgtccggc ggtccgccgg
     9241 cgacgaagag cgtcgcctcg gcgaggccgt aacaggggaa gaacgcctcc cgccggaagc
     9301 cgcagggggc gaacgcctcc gcgaaccgcc gcagcgtctc ggcgcgcacc ggctcggcgc
     9361 cgttgaaggc cacgctccag gacgacaggt cgagcccctc ccgctcttcc gggccgaggg
     9421 cgcggacgca gaggtcatag gcgaagttgg ggccgccgct ggtggtcccg ccgaagcgct
     9481 cgatcgcccg cagccagcgc gcgggccgct gcaggaacgc cgccggcggc atgagcacgc
     9541 aatcgcaccc gacgtaacag ggctggagca cgttgccgat cagccccatg tcgtggaaga
     9601 gggggagcca tcccacgacc accgactccg ccgtctggcg gaatgcgcgg cggatcgcct
     9661 ccagattgtg caggaggttg gcgtgggtga ccacgacccc cttgggcgtg cccgtggagc
     9721 cggaggtgta ctggaggaac gcgacctcct cggggcccgg ctcccggccg gcgtcctcgt
     9781 cctcgtccac cgtcccggtg tcgagccaga gcgcggacgc gagctccggg acctgcccgg
     9841 cgatcccctc gcggcggacg agcagcgccg cggtggtcag caccgcccgg ggacggcaat
     9901 cggccgcgac gccgcccagg cgcgggtccg gacgccggcc gtgcggcggc tggacgggga
     9961 cggcgatcac tccggcatac aggcagccga agaaggccgc gatgagctcc acgcccgacg
    10021 ggtagagcag gagcacccgc tcgccgcggg ggaccgaccg cgccagcacc gcggcgatgg
    10081 tgcgggcccg ccgctggagg ccgtcatagg agaggcgggc cccggcctcc tctccctggg
    10141 tgagaaaggt atagccgcgt cccggctgct ccgttgcctg acggtgaagg agctccacca
    10201 gggatttcat cggtcccgca cctcaagcct ctcgctcaag ccgacctccg gagtggtgca
    10261 tcggagcagg gggcacctgt atacagagtc cgccacaggc cgtcaactct cctggtgcgc
    10321 gccggagctc ctccggaaca ttgtgctacc atcccgccgc acttcgcctg cgaggggatc
    10381 tggtcatgga tgaacggcaa cagagcggca ggccgctggc ggcctcggga cgtgacgcgc
    10441 gcgtcatcgc catggtggtc ctggccctcc tgggcgcctg gatcagcttc catctcaccc
    10501 gctaccacct ggcgggcagc ggccatgggg gctcgggcgt cttccacacc gtctgcgaga
    10561 tgaccggcgg agggtgtgat cagatcctca agagcacctg gtccacgctg ccccggcaga
    10621 tcccgaccgc gttcgcgggg
//
LOCUS       DASPJI010000344         4159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_315620, whole genome shotgun sequence.
ACCESSION   DASPJI010000344 DASPJI010000000
VERSION     DASPJI010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315620"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VEL74_09085"
     CDS             <1..723
                     /locus_tag="VEL74_09085"
                     /inference="COORDINATES: protein motif:HMM:NF044415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine-dependent deoxygenase like protein"
                     /protein_id="HYG62723.1"
                     /translation="TTRAYLQIQATLHKRGTLHPVRLWNYIPGIHDPMHVDQDRYMSF
                     NAGRFEALCRWLGGAEQFDTLVASASGVGHDGQDLVIHCLAADSPGRAVANPRQIAPY
                     HYSQRYGPLPPCFARATLIEGRAGQPLVLVGGTASIVGEESVHAGDLARQTGETLTNL
                     AVLLREAAGTPIEPVIGPTGARAAALARFRDVRVYYRHPENLPELCRLLQDAFPAALV
                     EWVRADICREDLLVEIEGVAEL"
     gene            811..1671
                     /locus_tag="VEL74_09090"
     CDS             811..1671
                     /locus_tag="VEL74_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG62724.1"
                     /translation="MLRKLIVGLLLTSLLAPAALDAAQQREKRRGGGKKPKPGQQQQQ
                     QPAKPGSENPQQGQGNPPPAPEPPPAAIGTVDRKLLEYRTSEARSAINGVAGQADSNA
                     YVAAALGRVLEQEKSYGEAVTRLRKATELAPSDPAPWVHLGAVYARQNQKGEADAAFR
                     KATEVAQGRSDGDSLYYLGVAQQQLGQYDQAVQTLERARSGRESLASYQIGVTRAYQE
                     NWTAAVEQLDRAISADSGLALAYYYRGLANEKLGRKDKLINDMERFVDLAPSSPEADR
                     ARAILRAARR"
     gene            1891..3108
                     /locus_tag="VEL74_09095"
     CDS             1891..3108
                     /locus_tag="VEL74_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62725.1"
                     /translation="MKKNVMARSASAVIAGLLLAVLGSAMASDHDTVVVAVPARAGAV
                     DGTTQNSDEFIWNLFTQFAAPVYKEKPSPVVFETWASDLDTFAASPRWPSPDEPKKLQ
                     RSRLGAKTTPHHSPIDVPCNPPAGAAVGGFPTSGTPTPCIAEEVKRNRPQFDYIVSNN
                     LNTQVGLAAAYAKSFKVTMPTSAISVKGDWIPVQTMLQWIPQLGSVEKIRTLYYMATV
                     DSVEYALVSLHVSSRQNPNWVWGTFEHQMNPGRCDSIGCFDTFGSTIPVVRPNKATVN
                     AQYGACPKTEPLKALMAKANLSTVWENYCLKSSQVDYTAADGTPYVLGNSVIEGITGN
                     GTVAASSCIGCHVYASFGSTGTPTTAVQHILPYNPTGQPIPAVLDGSLQYDFMWGVLN
                     AAAPVTTATTSSK"
BASE COUNT          706 a         1472 c         1335 g          646 t
ORIGIN      
        1 accacccgcg cctacctcca gatccaggct accctccaca agcgcggcac cctccatccg
       61 gtccggctgt ggaactacat cccgggcatc cacgacccca tgcacgtgga ccaggaccgc
      121 tacatgtcct tcaacgccgg gcgcttcgag gcgctctgcc ggtggctcgg cggcgccgag
      181 cagttcgaca ccctggtggc gtcggcctcc ggcgtcggcc atgacgggca ggacctggtc
      241 atccactgcc tggcggcgga cagccccggc cgggcggtcg ccaacccgcg gcagatcgcc
      301 ccctatcact actcgcagcg atacggtccg ctgcccccct gcttcgcccg cgccacgctc
      361 atcgagggcc gcgccgggca gcctctggtg ctggtggggg gcaccgccag catcgtcggc
      421 gaggagtcgg tgcacgccgg agacttggcg cgccagaccg gcgagacgct gactaacctc
      481 gccgtcctgc tgcgcgaggc ggccggcacg cccatcgagc cggtgattgg tccgaccgga
      541 gcgcgggcgg cggcgctggc ccggttccgc gacgtgcggg tctattaccg gcacccggag
      601 aacctgcccg agctgtgccg cctgctccag gacgccttcc ccgccgcctt ggtcgagtgg
      661 gtccgggccg acatctgccg ggaggacctc ctggtggaga tcgaaggcgt cgccgagctg
      721 tgacgccgga gccttgaaag agcggcgccc ctgatagtgt tatcagggtg agccgccccg
      781 aaccgggagc ggccgggagg aatcgacccc atgctccgca aactgatcgt cggcctgctc
      841 ctgacttcgc tgctcgcgcc ggcagccctc gacgccgccc agcagcgcga gaagagaagg
      901 ggtggcggca agaagcccaa gccgggccaa cagcaacagc aacagccggc caagccgggc
      961 tcggagaacc cccagcaagg ccaggggaac cctccgccgg cccccgagcc gccgccggcc
     1021 gccatcggca ccgtcgaccg caagctcctg gagtaccgga cctccgaggc ccgctcggcg
     1081 atcaacggcg tggccggcca ggcggacagc aacgcctacg tcgccgcggc gctgggccgc
     1141 gtcctggagc aggagaagag ctacggcgag gccgtgaccc ggctgcgcaa ggccacggag
     1201 ctggcgccgt ccgatcccgc cccctgggtc cacctcggcg ccgtctacgc gcgccagaac
     1261 cagaaggggg aggccgacgc cgccttccgc aaggccacgg aggtggcgca gggcaggagc
     1321 gacggcgact ccctctacta cctgggcgtg gcgcagcagc agctcggcca gtacgaccag
     1381 gccgtccaga ccctggagcg cgcccgctcc ggacgcgagt ccctggcctc ctaccagatc
     1441 ggcgtcaccc gcgcctatca ggagaactgg accgccgccg tggagcagct cgaccgcgcc
     1501 atcagcgccg actccggtct ggccctggcc tactactacc gcggcctcgc caacgagaag
     1561 ctcggacgca aggacaagct gatcaacgac atggagcgct tcgtcgacct cgctccgagc
     1621 tcccccgaag ccgaccgcgc ccgcgccatc ctgcgggcgg cgcgccggta gaccggcccg
     1681 gtccgaaaga aagcccgccc cccgcggagg ctcccagcgc cgggagcccc tgcggggggc
     1741 gctctttttt gacaccggcg ggccgtgagg ctacactcct tccatcattt tctgtaacag
     1801 gctttctcgg cgtgacccgt ggaggaccgc gcgctcacgg gagcgttccg gagcgaagcc
     1861 attcgtctac cttcttttcg aggagatcgt atgaagaaaa acgtgatggc ccgatccgcg
     1921 tccgcagtca tcgccgggct tctcctggcg gtgctgggat ctgccatggc ctcggatcac
     1981 gacaccgtcg tggtcgcggt ccccgcgagg gccggagccg tggacgggac gacccagaac
     2041 tccgatgagt tcatctggaa cctgttcact cagttcgccg cgccggtgta caaggagaag
     2101 ccatcaccgg tggtgttcga aacctgggcg tccgacttgg acaccttcgc cgcctcgccg
     2161 cgttggccga gcccggacga gccgaagaag ctgcagcgga gcaggctcgg cgccaagacg
     2221 accccgcatc acagcccgat cgatgttcca tgtaatcccc ctgccggcgc ggcggtgggc
     2281 ggatttccga ccagcggcac gccgacgccc tgcatcgccg aagaggtgaa gcggaatcgc
     2341 ccgcaattcg attacatcgt cagcaacaac ctgaatacgc aggtgggctt ggcggccgcc
     2401 tacgcgaagt ccttcaaggt cacgatgccg acgtcggcca tctcggtcaa gggcgactgg
     2461 atccccgtgc agacgatgtt gcaatggatc cctcaactgg gcagcgtcga gaagatccgg
     2521 acgctctatt acatggccac cgtcgactca gtcgaatatg ccctcgtgtc tctgcacgtg
     2581 agcagccgcc agaacccgaa ctgggtgtgg ggaacgttcg agcaccagat gaatccgggc
     2641 cgctgtgaca gcatcggctg tttcgacact ttcggctcca ccatcccggt cgtccgcccg
     2701 aacaaggcca cggtcaacgc acaatatggc gcctgcccca agacggagcc gctgaaggca
     2761 ttgatggcca aggcgaacct ctcgaccgtc tgggagaact attgcctcaa gtcgagccag
     2821 gtggactaca ccgctgccga cggcacgccg tacgtgctcg gcaattcggt gatcgaaggg
     2881 atcacgggca acggcaccgt tgccgcctcg tcctgcatcg gttgccacgt ctacgcctcc
     2941 ttcggctcga ccggcacgcc gaccaccgcc gtccagcaca tccttcccta caatccgacg
     3001 gggcagccga tccccgccgt tctcgacggc tctttgcagt acgacttcat gtggggcgtc
     3061 ctgaatgcgg cggcgccggt cacgacggca acgacctctt cgaaatagtg gaaaaggagc
     3121 gctggctgcg tctgttcggt ctggctgtcc ttggggctcg ccatctactt cggctatgga
     3181 agacgccaca gccaggtggg gaagctggcg gggcgaggct tcgccgccgg tgtcctgatc
     3241 aagacacgtc caagcgcggc gcccgccccc cggcaccgcg cttcggacac gtacatcgat
     3301 cgtcaaagag ctggaggagc gactctcatt cgaccagagt cgcgctcctg tcagtcggca
     3361 gcataccaca gagtcgtgga gaatgattcc attgacgcag gcgagtcgtc gcttgtagtc
     3421 tctcctctga gctgccttcc tttcaggagc tgagtggacc cggatcggcg gcgtttctgt
     3481 gtgttccttg acaactgaat cgtgaaaggg ggcgccgccg tgtccaaggc cgcgctccct
     3541 gaggacacct ccgtccgaca ggaggacccc ggggaggcgg aggtccgatc cctccgcctc
     3601 ccactcggga gcacaacggt gcgctgccgg aacacaacgg cgcctggcga gatcgctatc
     3661 cccccgtcgc ggatcattcc cctggagctc tggatcgcaa tctccaccgg gggagtgttc
     3721 ccgccggccc gccggatcat tcccccgtgt tcccgaacct ctccgtgggc cgcggttgtg
     3781 atccggaacg ctgggagaat gaccctcccc gtgtccggga tcattcttcc gcaccgggta
     3841 gtgatctccc cgccttaccg aatgatctcg ccgagggcgc ttgtgatccg tggatgtcgc
     3901 ggggtgatgg tcgtgaggac tggaatcgat ggtggtggag gggggagcct ccggcagact
     3961 gaatccgccg aaggcaccct atagataagg atgtcctact cggggctcgc cgccgccgat
     4021 tccgccgcca cccgctcgtc gaggatggcg ccgcaccgtt cgagggtcac ggacccggcc
     4081 gcctccagca cgaaggccag agactcgggc gaccgcagca ggtggacgag cacgttcatg
     4141 gcgtccaccg ctcgctcct
//
LOCUS       DASPJI010000345         3453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_422319, whole genome shotgun sequence.
ACCESSION   DASPJI010000345 DASPJI010000000
VERSION     DASPJI010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422319"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3453)
                     /locus_tag="VEL74_09100"
     CDS             complement(<1..>3453)
                     /locus_tag="VEL74_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62726.1"
                     /translation="AKVAEAPEAVALVSAVGHAVTYGELGRRAARLAHCLRAAGVGPE
                     RRVGVLLDRSPEEVTSLLAVFEAGGAYVPLDPVWPRERVALVLEDAGVRVVLSTSNLA
                     DRLPGPSPLLLDRVWDKLDLWSSEPPAEGAAEVDHLAYVIYTSGSTGRPKGVEIEHRG
                     LLRLLAWHRRAFGVTAADRATRLSGLAFDAAVWELWPYLVAGASVHVPDEEVRTSPSA
                     LHDWLNAEGITIGFVSTPVAEALLPLSLTGPAAIPLRVLLTGGDRLHHDPGPLPFTLV
                     NNYGPTESTVVATSGAVQARGPGRAPDIGQPIDDTRVLLLDRELRLVPPGVAGEICIG
                     GPGLARGYLGQPGLTAERFVPDPYAAEPGERLYRTGDLARHLTDGAIEFLGRIDFQVK
                     IRGLRIELGEIEAALSAHPQVREAAVLVREDRPGEPSLVAYVAAHAEVSPEALRSWLR
                     GRLPEAMVPSAFVLLDSLPLTPNGKLDRKALEKIAPVRPDHDRLDAYVAPRTPAEEVL
                     AGIFAEVLRLERVSVEDGFFDLGGHSLLATQVISRVREAFGAELPVRELFEEPTAAGL
                     AGRLALLRDSTLAAPPIVSVPRPHGEDGELPLSFAQQRLWFLERLAPGSPAYNVPLAV
                     RISGPLSLRLLAQALEEVARRHEALRTTFAEREGHPVQVIAPLLRVPLEVVDLSGLPP
                     AQRETELVRRATAEALLPFDLEAGPLVRAALLRLAGEDHALLVTMHHIVTDGWSMGIF
                     LRELTALYGGRELPVLPVQYADFAAWQRAWLTGDVLAAQLGYWRQRLSGAPARLILPT
                     DRPRPPVETHRGASRPLDLPPALSRSVALLARREGATPFMVLLAGFAALLGRYSGQGD
                     VVVGSPIAGRNRREIEGLIGFFINTLALRVELADAPDFPSLLCQVRRTALDAYAHQDI
                     PFERLVEELVSERGGLDHSPLFQVMLTVQNTPAMTGEAEGLGVPGVELRRVTVESATA
                     KFELSLGFSPYGEELAGGLEYNTDLFDAATAERMARHFVRLLDAAVASPETPLPELPL
                     LAPAEERQILGEWNDTTVRFPESDRCLHELIREQARRRPEAVAVAIGDEQLSYGELQR
                     RADGLARRLVAMGAVPGSVVGVRLERSLELIVALVGVLEAGAAYLPLDPSYPQERLAF
                     LVEDA"
BASE COUNT          541 a         1356 c         1144 g          412 t
ORIGIN      
        1 gcgtcttcca ccaggaaggc caggcgctcc tgcggatacg acgggtccag aggcaggtag
       61 gccgcgccgg cttccagcac gcccaccagc gccacgatca gctccaggga gcgctccagg
      121 cggaccccca ccaccgaacc ggggacggcg cccatcgcca ccagccgccg cgccaggccg
      181 tccgcccgcc gctgcagctc gccatagctc aactgctcgt cgccgatggc cacggcgacc
      241 gcttcgggcc ggcggcgcgc ctgctcccgg atcagctcgt gcagacaccg atcgctctcc
      301 gggaacctca ccgtcgtgtc gttccactcg cccaggatct gccgctcctc ggccggcgcc
      361 agcagcggca gctccggcaa cggggtctcc ggcgacgcca ccgccgcgtc cagcagccgc
      421 acgaaatgcc gcgccatccg ctccgccgtg gcggcgtcga acaggtcggt gttgtactcc
      481 agcccgcccg ccagctcctc gccataggga gagaagccca ggctcagctc gaacttggcg
      541 gtcgcggact ccaccgtcac ccgccgcagc tccacgcccg gcacccccaa gccctcggcc
      601 tcccctgtca tcgcgggcgt gttctgtacc gtcagcatca cctggaagag cggcgagtga
      661 tccagtccac cccgctcgct caccagctcc tcgaccaggc gctcgaacgg gatgtcctgg
      721 tgcgcgtagg cgtccaacgc cgtccgccgc acctggcaca gcaggctcgg gaaatccgga
      781 gcgtccgcca gctccacccg caaagccagg gtattgatga agaagccgat cagcccctcg
      841 atctcgcgcc ggttgcggcc cgcgatgggc gagcccacca ccacgtcacc ctgaccggag
      901 tagcggccca gcagagccgc gaagcccgcc agcaggacca tgaacggcgt cgccccctca
      961 cgccgggcga gcaacgccac cgagcgcgac aacgccgggg gcagatccag cggccgggag
     1021 gcgccgcgat gggtctccac cggtggacgc ggccggtccg taggcaggat caggcgcgcc
     1081 ggcgccccgc tcagacgctg ccgccagtag ccgagctgag ccgccagcac gtcgcccgtg
     1141 agccaggccc gctgccaggc cgcgaagtcc gcgtactgca ccggcagcac cggcagctca
     1201 cgaccgccgt agagcgccgt cagctcgcgc aggaagatgc ccatggacca gccgtcggta
     1261 acgatgtggt gcatggtcac cagcagcgcg tggtcttctc ccgccagccg cagcagagcc
     1321 gcccgcacca acgggcccgc ttcgaggtcg aacggcagca acgcctcggc cgtggcccgg
     1381 cgcaccagct ccgtctcgcg ctgcgccggc ggcagaccgg acaggtccac cacctccagg
     1441 gggacccgta acaagggggc gatcacctgg acagggtgcc cctcccgctc ggcaaaggtg
     1501 gtacgcagcg cctcgtggcg ccgcgccacc tcctccagag cctgcgcgag caggcggagg
     1561 ctcagaggac cggagatacg caccgccagg ggcacgttgt aggccgggct gcccggcgcc
     1621 aaccgctcca ggaaccagag ccgctgctgg gcgaaggaca gcggcagctc gccgtcctct
     1681 ccgtgcgggc gcggtacgga cacgatgggc ggcgccgcca gcgtggagtc gcgcagcagg
     1741 gccagccgtc cggccagccc cgcggccgtc ggctcctcga acagctcccg caccggcagc
     1801 tccgcgccga aggcctcgcg tacccgcgag atcacctggg tcgccagcag cgagtgccct
     1861 cccaggtcga agaagccgtc ctcgacgctc accctctcga gccgcaggac ctccgcgaag
     1921 atccccgcca gcacctcctc ggccggggtg cgtggcgcca cgtacgcgtc gagccggtcg
     1981 tggtctggac gaaccggggc gatcttctcc agcgccttgc ggtccagctt gccgttggga
     2041 gtcagcggca gggagtccag cagcacgaac gccgacggca ccatcgcctc gggcaggcgg
     2101 ccgcgcagcc agctccgcaa ggcctccggg ctcacttcgg cgtgcgccgc cacgtaggcc
     2161 accaggctcg gctctccagg gcggtcttcc cgtaccagaa ccgccgcctc cctcacctgc
     2221 gggtgagccg aaagcgcagc ctcgatctcc cccagctcga tgcgcaggcc gcggatcttg
     2281 acctggaagt cgattcgccc caggaactcg atggccccat ccgtcaggtg gcgcgccagg
     2341 tctcccgtgc ggtacagccg ctcgccgggt tcggcggcat acgggtccgg cacgaagcgc
     2401 tcggcagtga ggcccggctg gcccaggtag ccccgggcca ggcccgggcc gccgatgcag
     2461 atctccccgg ccaccccggg cggcaccagc cgcagctccc ggtccagcag cagcacccgg
     2521 gtgtcgtcga tcggttggcc gatgtccggc gcccggccag gacctcgcgc ctggacggcg
     2581 cccgaggtgg cgacgaccgt gctctccgtc gggccgtagt tgttgaccag agtgaacggt
     2641 agcggaccgg gatcatggtg cagacggtcg ccgccggtca gcagcacccg cagggggatg
     2701 gcggccggac ctgtcagcga cagcggcagc agcgcttcgg caacaggcgt cgacacgaag
     2761 ccgatggtga tcccctcggc gttcagccaa tcgtgcagag cggagggcga ggtgcggacc
     2821 tcctcgtccg gtacgtggac gctggctccg gccaccaggt agggccacag ctcccagacc
     2881 gcggcatcga aagccaggcc ggacagccgg gtcgcccggt ccgctgccgt cacgccgaag
     2941 gcccgccggt gccaggccaa caggcgcagc agaccgcgat gctcgatctc cacccccttg
     3001 gggcggccgg tcgacccgga ggtgtagatc acgtaggcca ggtgatccac ctcggctgcg
     3061 ccctccgcag gaggctcgga gctccacagg tccagcttgt cccagacgcg gtccagcagc
     3121 agcgggctcg gccccggcag cctgtcggcg aggttcgaag tggacagcac cacccggacg
     3181 ccggcgtctt cgagcaccag ggccacgcgt tcgcgcggcc acaccggatc gagcggcacg
     3241 taggcgcctc ccgcctcaaa gaccgcgagc aggctcgtga cctcctccgg cgagcggtcc
     3301 agcagcaccc ccacccgccg ctccggccct acccccgcgg cgcgcagaca gtgggccagc
     3361 ctcgccgcac gccggcccag ctcgccataa gtcacggcgt ggccgaccgc cgagaccagc
     3421 gccaccgctt ccggtgcttc ggccaccttc gcc
//
LOCUS       DASPJI010000346         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_653359, whole genome shotgun sequence.
ACCESSION   DASPJI010000346 DASPJI010000000
VERSION     DASPJI010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653359"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2046)
                     /locus_tag="VEL74_09105"
     CDS             complement(<1..>2046)
                     /locus_tag="VEL74_09105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62727.1"
                     /translation="PRSAAQSFHGASLPVRLPAGLTRELQALGRREGATLFMVLLAGF
                     QTLLGRYSGQQDLAVGTPVAGRNHVEIEDLIGFFVNTLVLRADLSRGAAGEPSFRELL
                     GQTRETALDAHTHQDLPFDRLVQELSPERSLAHVPLFQVMLALQSLPVDRVEMGGLVL
                     TPLEADAGTAKFDLSLSLEEKDGELAGSLRYSTDLFDRPTVLRMMDHLERLLATVAAA
                     PEQRLADLEIFSAAQRAQLLVEWNDSAQEAPGLPVHRLFARQAERTPAAVAVRTDGGS
                     LTYGELAARVNAWAVHLRALGAGPEQVVAVCLERSSDLVAALLAVLTSGAAYLPLDPR
                     DPEERREQILADARPLLSLTRSALAAGSGGSWAGPSLFVEDLPAVADEAVHSEDRPVP
                     AGTLAYVLYTSGSTGRPKGVAVEHASLSGYLAWIAGLLEGAGVRWLPLLSSVTFDASL
                     KQLFAPLARGEAVRVISEDALLRPVRLLEILGEAPGAGCNTVPTLWDSLLGTLERGEA
                     APPADLRALFLGGERLPEELAARTRRLLPGIAIYNLYGPTEATSNAAWSRLPEAGETR
                     RVVLGRPVGGAHIVLVDRHLRPVPPGAVGELCAGGTGVARGYRERPGLTAEQFVPDPF
                     GGGPGARLYRTGDLARRLPDGSLDFLGRLDHQVKVRGFRIELGEIEAVLEEHPEVRAA
                     "
BASE COUNT          313 a          749 c          748 g          236 t
ORIGIN      
        1 gccgcgcgca cctcgggatg ctcctccaag accgcctcga tctctcccag ctcaatacgg
       61 aagccccgca ccttgacctg atggtccagg cggccgagga aatcgaggga gccgtcgggc
      121 agacggcgcg caaggtcgcc tgtgcggtac agccgcgcac cgggaccgcc gccgaaggga
      181 tcggggacga actgctcggc ggtcagcccg ggcctctccc ggtaaccgcg ggcgactccg
      241 gtgccgccgg cgcagagctc gcccacggcg cccggcggca ccggccgcag gtgccggtcc
      301 accagaacga tatgagcgcc ccctacgggc cggccgagga ctacgcggcg ggtttcgccg
      361 gcctccggca ggcgggacca ggcggcattg gaggtcgcct cggtcgggcc gtagaggttg
      421 tagatggcga tgccgggcag gagccggcgc gtgcgggcgg ccagctcttc cggcagccgc
      481 tcgccgccca ggaagagggc gcgcaggtcc gccggcggcg ccgcctcgcc ccgctccagc
      541 gtgccgagca gagagtccca cagcgtcggc acggtgttgc agccggcgcc gggcgcctcg
      601 cccaggatct ccagcaggcg caccggccgc agcagcgcgt cctcggagat cacgcgcact
      661 gcctcgccgc gcgccagagg ggcgaagagc tgcttgagcg atgcgtcgaa ggtcactgag
      721 ctcaggaggg gcagccagcg caccccggcc ccctcgagga ggccagcgat ccaggccagg
      781 taaccggaca ggctggcgtg ctccaccgcc acccccttcg gccggccggt ggagcccgag
      841 gtgtagagga cataggcgag ggtcccggcg ggcaccggcc ggtcttcgga atgcacggcc
      901 tcgtccgcca ctgccggcag atcctccacg aagagcgacg ggccggccca ggagccgccg
      961 gagccggcgg ccagagcgct ccgggtcagc gacaggagcg gccgggcatc ggcgaggatc
     1021 tgctcccggc gctcctcggg atcgcgcggg tccagaggga gataggcggc gccggaggtc
     1081 aggaccgcca ggagggccgc caccaagtcg gaggagcgct ccaggcagac ggccaccacc
     1141 tgctccggcc cggcgccgag cgcgcgcagg tggacggccc aggcgttcac ccgcgccgcg
     1201 agctcgccat aggtgaggct gccaccatcc gtgcggaccg ccaccgccgc gggcgtacgc
     1261 tccgcctgcc gcgcgaagag acggtgcacc ggcagccccg gcgcctcctg cgccgagtcg
     1321 ttccactcca ccaggagctg ggcgcgctgc gccgcggaga agatctccag gtccgccagg
     1381 cgctgctccg gcgcggccgc gaccgtggcg agcagccgct ccagatggtc catcatccga
     1441 aggacggtcg gacggtcgaa gagatcggtc gaatagcgca acgagcccgc cagctccccg
     1501 tccttctcct ccagagagag ggagaggtcg aacttggccg tacccgcgtc cgcttccagg
     1561 ggggtgagga cgaggccccc catctcgacc cggtccacgg gcaggctctg cagggcgagc
     1621 atcacctgga acagggggac gtgggcgagg ctcctctccg gcgagagctc ctggaccagc
     1681 cgatcgaacg gcaggtcctg gtgcgtgtgg gcgtctagcg cggtctcgcg ggtctggccg
     1741 aggagctcgc ggaacgacgg ctcccccgct gcccctctcg acaggtcagc acgcagtacc
     1801 agcgtgttga cgaagaaccc gatgaggtcc tcgatctcca cgtggttgcg tccggcgacc
     1861 ggggtgccca ccgccaggtc ctgctgcccg ctgtagcgcc ccaggagagt ctggaagccg
     1921 gccagcagca ccatgaagag cgtcgctccc tcgcggcggc cgagcgcctg cagctcccgg
     1981 gtcagcccgg ccggcagccg cacgggcaac gaggcgccat ggaagctttg ggctgccgac
     2041 ctcggc
//
LOCUS       DASPJI010000347         8510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_365185, whole genome shotgun sequence.
ACCESSION   DASPJI010000347 DASPJI010000000
VERSION     DASPJI010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8510)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8510
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365185"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1927
                     /gene="thiC"
                     /locus_tag="VEL74_09110"
     CDS             17..1927
                     /gene="thiC"
                     /locus_tag="VEL74_09110"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HYG62728.1"
                     /translation="MIELAPAPSAPSESAEPRRLDPALTRPLPNSTRIYVEGTLPGVR
                     VPMREVRLSPTRPRDGAAVDNPPVTVYDTSGPYTDPAAGIDVRRGLPPLRLGWIAGRG
                     DVEGLPQVSSEYGRRRAADPRLADLRFQHIRKPLRGLPGSNVTQMHYARKGIVTPEME
                     FIAIRENQRRELAREETGGKAWGGGVAQHPGQSWGARILERITPEFVCDEVARGRAII
                     PSNINHPESEPMIIGRNFLVKINANIGNSAVTSSIEEEVGKMIWAIRWGADTVMDLST
                     GKNIHETREWILRNCPVPVGTVPIYQALEKVGGKAEDLTWEIYRDTLIEQAEQGVDYF
                     TIHAGVRLGYVPLTAGRMTGIVSRGGSIHAKWCLAHHRESFVYTHFEEICEIMKAYDV
                     AFSLGDGLRPGSIADANDAAQFAELETLGELTKVAWSHDVQVMIEGPGHVPMHLIQEN
                     MEKQLAACGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGL
                     PNREDVKAGVIAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRWEDQFNLSLDPDTA
                     RDFHDETLPQEVAKVAHFCSMCGPHFCSMKITEEVRDYAARQQLDETAALEAGMREKS
                     EEFREAGAEIYR"
     gene            complement(1918..3048)
                     /locus_tag="VEL74_09115"
     CDS             complement(1918..3048)
                     /locus_tag="VEL74_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HYG62729.1"
                     /translation="MTDQAPLLMIPGPVELSPAVRAAASQAPPGHTTPRFIESFATCL
                     ERMRQVWKAAGSSQPFILAGSGTLAMETAAANLIEPGDKALVVNSGYFSDRMVEILRR
                     YGAEVAEVAAAVGEAPAPEEVRQMLERGSPFKALFATHVDTSTGVRVDPEPLCRIARE
                     AGVLSVLDGVCATAGERFEMEAWNADLYFTASQKAIGVPPGLALLVASERALAVRASR
                     KAAPPALYLDWDAWLPIHRAYEERRPSYFATPSTSLVLALEAGLGEILENGIDERIAA
                     HARAAETLRAAWRDLGLRFVPRTEAETANTLSALLFPDGVDASLLPRIAARGVIIAGG
                     LHPAVRTTSFRVGHMGYTVTRPDYLQRSIEAVTGALREAGYR"
     gene            complement(3068..5188)
                     /locus_tag="VEL74_09120"
     CDS             complement(3068..5188)
                     /locus_tag="VEL74_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62730.1"
                     /translation="MSGDDPRKRRSRELAERLAMPEGLRAARLSPHPLAPSPIPSPQP
                     GEGEGPKGRRAPEPISIWQPQMLEDSLAFVRPEPSPLGDHHAATRVLRPKRAELPLRV
                     CFFGESVAAGYLYAPHLTPAGVLERQLRAVGGADNFEVIDLARTNETLPSLAATVRSA
                     LQIQPDMLVLFVGNNWNLLETPEVSPYAPSVRARQRYAEALRKSGLLGPVWLAADELR
                     RSAEATLGFIADIARTIGIPVIVVIPEVNLVDWETRQPLTWFPGDAAARWYALYDRTQ
                     ALLDGGAFAEAASVAREMLDLDGGTCSTSWRLLARARLGEGDLDEARRACLAEIDASA
                     YATLGFLSAPQATTAARDLLKEAARRPGVFSVDLRQVFAEHTGSALPDRRLFLDYCHL
                     TAEGIHVAMAAVAAEVLNLSGMREQDVAWPELLPRLPAPDLAPEVEATARLGAAIHTA
                     HRMLPVGPKRPILEYWLEAALDASPGIEATMLDLLAVRAAPCPAVLTAEQQRNLDSPY
                     RLTLQHGWRWDHLDADLLQAIVEVLERRGRPAREELTRHLLAHHALREDGTELAHPAF
                     YHWEPLERFYPEVMSFEDLAQRATYRSPWPVSSFCLICDGERDLELDLTARLSRTGPV
                     HLSIDGEDVGTAEVGERWTRAVLRVDRRLLRPGLNRLALRWPEPPPRGEEALQQAIER
                     LELGIAADLHPVFGEVFSLIARPC"
     gene            complement(5185..6993)
                     /locus_tag="VEL74_09125"
     CDS             complement(5185..6993)
                     /locus_tag="VEL74_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HYG62731.1"
                     /translation="MNVLGISAFFHESACCLLRDGHLVAAAEEERFSRVKHDPRLPSS
                     AFRFCLRQGGIGITDLDAVAYYESPVEKLSRQLWAAMPEGGEGEEGLPPDLPWLDPGQ
                     PERAIREGLGWEGPLRIYRHHLSHAASAFFFSGFPEAALLTVDGVGEWATTTYGRGEG
                     AAVELFEEVEFPHSLGLLYSTITSYLGFEVNDGEYKVMGLAPYGRPRYLDQMRALVAS
                     EPGGQYRLDLSYFDFVDFMRGRRMYSERLCDLFGAPPRMRESEITPFHQDVARSLQVA
                     LEEILLEKARYLHERTGSRHLCMAGGVALNVVANGRILREGPFERLFVQPAAGDSGGC
                     LGAAALAYAEISELTGSRPSYEPLRHVYLGPSWAADEVARLLEATGLRALDFRGREAE
                     LLEAVVDRLEQRQVVGWFHGAMELGPRALGARSLLADPRDPEMRERLNRLVKKREAFR
                     PFAPSVLAAHAAGHFDLDHPSPFMLETCQVTSPLDLPAITHVDGSARPQTVDAAVAPR
                     YAALLEAFHRRTGCPVLVNTSFNVRGEPIVCSPVDALFCLGSSGIDCLVLEDFLIDRR
                     DLPPNWADLLPTWRERRRSGFARSEGAVSEDLYTFV"
     gene            complement(7185..8354)
                     /locus_tag="VEL74_09130"
     CDS             complement(7185..8354)
                     /locus_tag="VEL74_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HYG62732.1"
                     /translation="MPAPLRLIATCALGLEETVEAELVSLGVSDPERQRGAVAFSGTW
                     QDCWRANWRLRTANRVLVELATWPGRDGAELAAGARALVGGRTGKRPKGPQGGENDLG
                     ALFHPDRSFRIQATSTASEVRDVRWAALSVKDGLVDGQRDRYGRRSDVSREDPDLHLR
                     VRLHQDRATLLLDTSGEPLDRRGYRAVTSAAPVREQLAAACVLASGWDGRGPVVDPMC
                     GSGTLLIEAAWIALGRAPGHLRRSWAFQRLPGFDARAFEAIRRETIPTPGPDVHLLGN
                     DRSAEALAAARTNLERAELAGRATLTRGDAFALAPPAGPGLVVVNPPHGERIGADAGA
                     WRALGDLFKQQFKGWTAVVLAGGPDRGKHIGLRPRRRIPVMNGPLEGRILVFDLF"
BASE COUNT         1323 a         2913 c         3158 g         1116 t
ORIGIN      
        1 gacaggagag gacaccatga tcgagctcgc gccggcgccg tccgcgccat ccgagtcggc
       61 ggagccccgc cgtctggacc ccgccctcac ccggccgctg cccaactcta cccggatcta
      121 cgtcgagggg accctgccgg gcgtgcgcgt gccgatgcgc gaggtccggc tctctcccac
      181 ccgcccccgc gatggcgccg ccgtagacaa cccgccggtg accgtctacg acacctccgg
      241 gccctatacc gatccggcgg ccgggatcga cgtgcggcgc ggcctgccgc cgctgcggct
      301 cggctggatt gcgggccggg gcgacgtcga agggctgccg caggtctcct ccgagtacgg
      361 gcggaggcgg gcggccgacc ctcgccttgc ggacctccgt tttcagcaca tccgcaagcc
      421 gctgcgaggc ctgccgggct ccaacgtcac ccagatgcac tacgctcgca aagggatcgt
      481 caccccggag atggagttca tcgccatccg cgagaatcag cggcgggagc tggcccgaga
      541 ggagaccggc ggcaaggcct ggggtggcgg ggtggcccag caccccgggc agtcctgggg
      601 ggcgcgcatc cttgagcgca tcaccccgga attcgtgtgc gacgaggtgg cgcgcggccg
      661 ggcgatcatc ccgtcgaaca tcaaccatcc ggagagcgag ccgatgatca tcggccgcaa
      721 cttcctggtg aagatcaacg ccaacatcgg caactccgcc gtcacctcct cgatcgagga
      781 ggaggtgggc aagatgatct gggccatccg ctggggcgcc gacacggtca tggacctgtc
      841 gacggggaag aacatccacg agacgcggga gtggatcctg cgcaactgcc cggtgccggt
      901 gggaaccgtg ccgatctatc aggcgctgga gaaggtgggg ggcaaggccg aggacctcac
      961 ctgggagatc tatcgcgaca ccctgatcga gcaggcggag cagggggtgg actacttcac
     1021 catccacgct ggcgtgcggc tgggctacgt gccgctgact gccggccgca tgaccggcat
     1081 cgtctcgcgc ggcggctcga tccatgccaa gtggtgtctg gcgcaccacc gggagagctt
     1141 cgtctacacg cacttcgagg agatctgcga gatcatgaag gcctacgacg tggccttcag
     1201 cctgggggac ggcctgcgcc ccggctccat cgcggacgcc aacgacgccg cccagttcgc
     1261 cgagctcgag actctcggag agctgacgaa ggtagcctgg agccatgacg tccaggtgat
     1321 gatcgagggg ccgggccacg tccccatgca cctgatccag gagaacatgg agaagcagct
     1381 cgcggcgtgc ggcgaggccc ccttctacac cctggggccg ctgaccaccg acatcgcccc
     1441 cggctacgac cacatcacct cggccatcgg cgcggcgatg atcggctggt atggcacggc
     1501 catgctctgc tacgtcaccc cgaaggagca cctgggactg ccgaaccggg aggacgtcaa
     1561 ggcgggcgtc atcgcctaca agatcgcggc gcacgcggcg gacctcgcca aagggcaccc
     1621 cggcgcgcag gtgcgggaca acgcgctatc caaggcgcgc ttcgagttcc gctgggagga
     1681 ccagttcaac ctctcgctgg acccggacac cgcccgcgac ttccacgacg agaccctgcc
     1741 gcaggaggtg gccaaggtgg cccatttctg ttccatgtgc gggccccact tctgttcgat
     1801 gaagatcacc gaggaggtgc gggactacgc cgcccgccag cagctcgacg aaacagcggc
     1861 gctggaggcc gggatgcggg agaagtccga ggagttccgg gaggcggggg cggagatcta
     1921 ccggtaaccg gcttctcgaa gcgctccggt caccgcctcg atgctccgtt gcaggtagtc
     1981 cggccgcgtc accgtgtagc ccatgtggcc gacgcggaac gaggtggtcc gcaccgccgg
     2041 atggaggccg ccggcgatga tgacgccgcg ggcggcgatg cgcggcagga gggaggcgtc
     2101 gacgccgtcg gggaagagga gcgcgctcaa ggtgttggcg gtttcggcct cggtgcgcgg
     2161 cacgaagcgg agccccaggt cccgccaagc ggcgcgcagg gtctcggcgg cgcgggcgtg
     2221 cgccgcgatc cgctcgtcga tgccgttctc caggatctca ccgaggccgg cttccagggc
     2281 caacaccagc gacgtcgagg gggtggcgaa gtagctcggc cgccgctcct cgtaggcgcg
     2341 gtggatgggg agccaggcat cccagtccag atagagagcg ggaggggcgg ccttgcggga
     2401 ggcgcggacg gccagggcgc gctcgctcgc caccaggagc gcaagccccg gcggcacgcc
     2461 gatcgccttc tgggaggcgg tgaagtagag gtccgcgttc caggcctcca tctcgaaccg
     2521 ctcgccggcc gtggcgcaga ccccgtctaa gaccgagagg acgccggcct cgcgggcgat
     2581 ccggcagagc ggctcgggat cgacgcggac gccggtcgag gtgtccacgt gagtggcgaa
     2641 gagggctttg aacggactgc cgcgctccag catctgccgg acctcctcgg gggcgggggc
     2701 ctcgcccact gcggccgcga cctcggccac ctcggcgccg taacggcgga ggatctccac
     2761 catgcggtcc gagaaatagc cgctgttgac caccagcgcc ttgtcgcccg gctcgatcag
     2821 gttggccgcc gcggtctcca tggccagcgt gccgcttccc gcgaggatga acggctggct
     2881 ggagcccgcg gccttccaga cctggcgcat ccgctccagg caggtcgcga acgactcgat
     2941 gaagcgggga gtggtatgcc caggaggtgc ctgggaggcg gcggcgcgca cggccgggga
     3001 gagctccacc gggccgggga tcatcaggag cggggcttgg tcggtcatgg gccgggatcg
     3061 tagcagctca acagggccgg gcgatcagcg agaacacctc gccgaagacc gggtggagat
     3121 cggcggcgat ccccagctcc aggcgctcga tcgcttgttg gagcgcctcc tcccctcgcg
     3181 ggggaggttc cggccagcgc agggcgaggc ggttgagccc cgggcggagg agacggcggt
     3241 ccacccgcag gacagcccgg gtccagcgct cgcccacctc ggccgtcccg acgtcctcgc
     3301 cgtctatcga gaggtggacg ggcccggtcc gggacaggcg ggcggtcagg tccagctcca
     3361 gatcgcgctc gccgtcacag atcagacaga agctcgacac cggccagggt gatcgatagg
     3421 tggcgcgctg ggcgaggtcc tcgaagctca tgacttcggg ataaaacctt tccaacggct
     3481 cccagtgata gaacgccgga tgagccagct cggtcccgtc ctcgcgaaga gcgtgatggg
     3541 cgaggaggtg acgggtcagc tcttcccgcg ccggccggcc acggcgctcc agcacttcca
     3601 cgatcgcctg gaggaggtcg gcgtccaggt gatcccatcg ccagccgtgc tggagggtca
     3661 ggcgataggg ggagtcgagg ttgcgttgct gctcggcggt gagcacggcc gggcacgggg
     3721 cggcgcggac ggcgaggagg tcgagcatcg tcgcttcgat gccgggggag gcgtccaggg
     3781 ccgcttccaa ccaatactcc aggatcggcc gcttgggccc caccggcagc atgcggtgcg
     3841 cggtgtggat ggcggcgccc agcctagccg ttgcctccac ctccggcgcg agatcgggag
     3901 ccggcaggcg ggggaggagc tccggccagg ccacgtcctg ctcccgcata cccgagaggt
     3961 tcagcacctc ggcggccacg gcggccatcg ccacgtggat gccttcggcc gtgaggtggc
     4021 aatagtcgag gaacaggcgc cggtccggga gggccgatcc ggtgtgctcg gcgaagacct
     4081 gccgcaggtc tacggagaag acgccaggcc gccgcgccgc ctccttcaga agatcgcggg
     4141 ccgccgtcgt ggcctggggg gcggagagga aacccagggt ggcataggcg ctggcgtcga
     4201 tctcggcgag acaggcccgc cgcgcctcgt ccaggtcccc ttcgccgagc cgcgccctgg
     4261 cgaggagacg ccaggaagtg gagcaggtcc cgccgtcgag gtcgagcatc tcccgggcga
     4321 cggaggcagc ttcggcaaag gcgcctccgt cgaggagcgc ctgggtacgg tcgtagaggg
     4381 cataccagcg ggcggcggcg tcgccgggga accaggtcag aggctggcgg gtctcccagt
     4441 ccaccaggtt gacctctggg atcaccacga tcaccgggat gccgatggtc cgggcgatgt
     4501 cggcgatgaa gcccagcgtc gcctcggccg accggcgcag ctcgtcggcg gcgagccaga
     4561 cggggccgag gaggcccgac ttcctcagcg cttcggcgta gcgctgccgg gcgcgcaccg
     4621 acggggcata gggggagacc tcgggcgtct ccagcaggtt ccagttgttg cccacgaaga
     4681 ggaccagcat gtccggctgg atctggaggg cgctgcgcac cgtggcggcc aggctcggga
     4741 gtgtctcgtt ggtgcgggcg aggtcgatca cctcgaagtt gtccgcgccg cccacggcgc
     4801 ggagctgccg ctcaaggacg cccgcgggcg tcaggtgcgg ggcatagagg tagccggcgg
     4861 ccacggactc gccgaagaag cagacccgca gggggagctc cgcgcgcttg ggccggagca
     4921 cccgggtggc cgcgtgatgg tcccccagcg gcgacggctc ggggcggacg aaggcgaggc
     4981 tgtcctccag catctggggt tgccagatgg agatgggttc gggggcgcgt ctcccctttg
     5041 gaccttcccc ctctcccggt tggggggagg gaatgggaga gggggccagg gggtgagggg
     5101 acagccgtgc cgcgcgcagc ccctccggca tcgccagccg ctcggccagc tcgcggctgc
     5161 ggcgcttcct gggatcgtcg ccgctcatac gaaggtgtag aggtcttcgc tgacggcgcc
     5221 ctcacttcgc gcgaagccgc tccggcgccg ctccctccag gtgggcaaca ggtcggccca
     5281 attcgggggg aggtcccggc gatcgatcag gaaatcttcg agcaccaggc agtcgatgcc
     5341 cgagctgccc agacagaaga gggcgtccac cggcgagcag acgatgggct ccccgcgcac
     5401 gttgaacgag gtgttgacca gcaccgggca gcccgtgcgg cggtggaagg cctcgagcag
     5461 ggccgcgtag cgaggagcta cggccgcgtc cacggtctgg gggcgcgccg agccgtccac
     5521 gtgggtgatc gccggcaggt ccagcggcga ggtgacctgg caggtctcca gcatgaacgg
     5581 cgacgggtga tcgagatcga aatgcccggc ggcgtgggcg gccagcacgc tcggggcgaa
     5641 gggccggaac gcctcgcgct tcttgaccag acggttgagg cgctcgcgca tctccgggtc
     5701 ccgcggatcg gccaggaggc tccgcgcccc cagcgcccgg gggccgagct ccatggcgcc
     5761 gtggaaccat ccgaccacct gccgttgctc cagccggtcc accacggcct ccagcagctc
     5821 cgcctcccgg ccgcggaaat ccagcgcccg cagaccggtg gcttcgagga gacgggcgac
     5881 ctcgtccgcg gcccagctcg gccccaggta gacgtgccgg aggggctcgt aggacggccg
     5941 gctccccgtc agctcactga tctcggcata ggcgagggcc gcggcgccga gacagccccc
     6001 ggagtcaccg gccgccggct gcacgaagag gcgctcgaac ggcccctccc gcaggatgcg
     6061 gccgttcgcc accacgttca gcgccacgcc gccggccatg caaaggtgcc tggaccctgt
     6121 ccgctcgtgg agatagcggg ctttttcgag gaggatctcc tccagcgcca cctggaggct
     6181 ccgcgccacg tcctggtgga acggggtgat ctccgactcg cgcatccggg ggggcgcgcc
     6241 gaagaggtcg cacagccgct ccgagtacat gcggcggcca cgcatgaagt cgacgaagtc
     6301 gaaataggag aggtccagcc ggtactgccc gcccggctcc gaggcgacga gcgcccgcat
     6361 ctgatccagg tagcgtggcc ggccgtaggg ggcgagcccc atcaccttgt actcgccgtc
     6421 gttgacctcg aagcccaggt aggaggtgat cgtcgagtag aggagcccga gggagtgcgg
     6481 gaactccact tcttcgaaga gctcgaccgc cgccccctca ccccgcccgt acgtggtggt
     6541 cgcccactcg ccgacgccgt cgaccgtcag cagcgccgcc tcggggaaac cggagaagaa
     6601 gaaggcgctg gcggcgtggg agaggtggtg ccggtagatc cgtagcgggc cctcccagcc
     6661 gagcccctcg cggatcgccc gctcgggttg cccggggtcc agccagggga ggtccggggg
     6721 cagcccctcc tctccttccc ctccttccgg catcgccgcc cagagctggc gcgacagctt
     6781 ctcgaccggc gactcatagt aggccaccgc gtccaggtcg gtgatcccga tccccccctg
     6841 gcgcaggcag aagcggaacg ccgacgaggg cagccggggg tcgtgcttga cccgggagaa
     6901 gcgctcctcc tcggccgcgg ccaccagatg cccgtcccgc agcaggcagc aggccgactc
     6961 gtggaagaag gcggagatcc cgaggacgtt cacggccggg gattctacag aaaaaggccg
     7021 ccggccccgg ggacgggccg gcggtgaagc ggacgatggc acacggccgg gacggccgtg
     7081 tggtgaggcg tgccatcgcc cgccaaatta gacggacacc cggcgcgcgc tcccttccgc
     7141 gcttggagag ggaatatagc agaaaattct aaataacgga tggatcagaa gaggtcgaag
     7201 acgaggatgc ggccttcgag agggccgttc atcaccggga tgcgccgccg gggacggagg
     7261 ccgatgtgct tgccgcggtc ggggccgccg gcgaggacca cggcggtcca ccccttgaac
     7321 tgctgcttga agaggtcgcc gagcgcgcgc caggcgccgg cgtcggcgcc gatccgttcg
     7381 ccatgggggg gattgaccac gacgagcccg ggaccggccg ggggggcgag cgcgaaggcg
     7441 tcgccgcggg tgagggtggc gcggccggcc agctccgccc gctccaggtt ggtccgggcg
     7501 gcggcgaggg cctcggcgct gcggtcgttg cccaggagat ggacgtccgg tccgggcgtg
     7561 gggatcgtct cgcggcggat ggcctcgaag gcgcgggcgt cgaagcccgg gaggcgctgg
     7621 aaggcccagc tccggcgcag gtgacccgga gcgcggccca gggcgatcca ggcggcctcg
     7681 atgagcagcg tgcccgagcc gcacatggga tcgacgaccg ggccccggcc gtcccagccg
     7741 gaagcgagca cgcaggcggc ggcgagctgc tcgcgcaccg gcgccgcgga ggtcaccgcg
     7801 cggtagccgc gccggtccag gggctcgccg gaggtgtcga ggagcagcgt cgcccggtcc
     7861 tggtggagcc gcacccgcag gtggaggtcc gggtcctcgc ggctcacgtc ggagcgccgg
     7921 ccgtagcggt cgcgctggcc gtccaccagg ccgtccttga cggagagcgc cgcccagcgc
     7981 acgtcccgga cctccgaggc ggtcgaggtg gcctggatcc ggaagctccg gtccgggtgg
     8041 aacagggccc ccaggtcgtt ctcgcccccc tgcggcccct tcgggcgctt ccccgtacgg
     8101 cctcccacca gggcgcgggc ccccgccgcc agctcggcgc cgtcccgccc cggccaggtc
     8161 gccagctcca ccagcacacg gttggcggtg cgcagacgcc agttggcgcg ccagcagtcc
     8221 tgccaggtgc cgctgaaggc gacggcgccg cgctggcgct cgggatcgga gacaccgagc
     8281 gacaccagct cggcttcgac ggtctcctcg aggccgaggg cgcaggtggc gatgagtcgg
     8341 agaggcgcag gcatggatca gtccccctgc ccggagacgc gggcgaggaa gtccttcatc
     8401 agccgcagga gctcctccgg ccgctcttcc atgggcagat ggccggtctc ctccaaggcg
     8461 acgaactcac cgcgggggag gcgcgccgcc gcggcccgcc catctcccac
//
LOCUS       DASPJI010000348         3461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_315843, whole genome shotgun sequence.
ACCESSION   DASPJI010000348 DASPJI010000000
VERSION     DASPJI010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315843"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VEL74_09135"
     CDS             complement(<1..459)
                     /locus_tag="VEL74_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62733.1"
                     /translation="MTLRDVRAPLLDLATVLAREAEPLALAGNAPLSLDSADSVCFVE
                     EGTVEVFAVPLDGSGPRTHLCTVPAGQLLCGAEPDGNGLGFLAVGHTGTRLSRLDLSR
                     LQELAQDPDTAADLAGRLDAWLIRLAAGVFPAAVPRVFEELRAGINIYIEE"
     gene            complement(467..2680)
                     /locus_tag="VEL74_09140"
     CDS             complement(467..2680)
                     /locus_tag="VEL74_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015130247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="HYG62734.1"
                     /translation="MPSLSRLLSWKKKEGAGEAAPPEAVRSPTVLQMEAVECGAAALA
                     IVLAHHGRWVPLEELRIACGVSRDGSKASNIVKAARQYGLEAKGYKQEPQSLRKHRPP
                     MILHWNFNHFVVLDGFHKGQVRINDPGMGPRVVTEEELDQAFTGVVLTFKPGASFEKS
                     GEAPRLVPALRRRLTGAAAALAFVILAGLALVIPGLVIPSFSKVFVDGVLLEGRRDWL
                     PPLLLGMGAAALVTGVLTWLQQTYLLRLETRMAVAGSSRFLWHVLRLPAEFFSQRFAG
                     DISSRVAINDRVAQLLSRDLATNALGALMIAFFAVVLVQYDPVLTLVGIAVVSLNVVA
                     LRFVSRKRVDGNRRLVQDQGKLVGTAIGGLQTIETLKATGGESDLFTRWAGYQAKVVN
                     GRQELERYTQLLDAVPPMLTAVNTALILGIGGLRVMNGDLTLGGLVAFQLLMVSFVTP
                     VNRLVSLGGRLQMVEGDMNRLDDVLRYRLDDSVKAGEDPRLLAEAPVKLTGHLELRGV
                     SFGYSRLDPPLIEDFDLLLRPGSRVALVGGSGSGKSTLAKLVTGLYRPWSGEILLDGR
                     PRADLPGTVVANSMAVVDQNVFLFEGTVRENLTLWDSTLPLPEVVAAAKDACIHEEIA
                     ARPGGYNSPVEEGGANWSGGQRQRLEIARALVGRPTLLVLDEATSALDPLTEAAIDEN
                     LRRRGCTCLIIAHRLSTIRDADEIIVLDKGKVVQRGTHDELKGVEGSYSRLISTE"
     gene            complement(2680..>3461)
                     /locus_tag="VEL74_09145"
     CDS             complement(2680..>3461)
                     /locus_tag="VEL74_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NHLP bacteriocin system secretion protein"
                     /protein_id="HYG62735.1"
                     /translation="RDVELARDQLQAEQELLKDGLVTRQTLVTSEQRLNSAQDQLAQA
                     RLELNGLELSRLEAEQQVDQQIEVRQAQIRDLELELRERQARLEESARVVAPRAGRVV
                     ELLVDRGDVVNPGTPLLSLEFPAEDLMAVLFVPASAGKKVQRGMPARITPSTVKREEY
                     GFIQGKVVSAGEFPASQRGMVRLLGNEALVQKLMAEGPPIQVNITLDQDTKTPTGYRW
                     SSSTGPNLRISSGTLAAGSVIVRRERPAALVLPTLREKLGL"
BASE COUNT          519 a         1204 c         1229 g          509 t
ORIGIN      
        1 ttcctctata tagatgttga taccggcgcg cagctcttcg aagactctgg gcacggcggc
       61 ggggaagaca cccgcggcga ggcggatcag ccaggcgtcc aggcggccgg ccaggtccgc
      121 ggcggtgtca gggtcttggg ccagctcctg gagccgggag aggtccaggc ggctcaggcg
      181 ggtgccggtg tggccaacgg ctaggaagcc caggccgttc ccatccggtt cggcgccgca
      241 gaggagctgg ccggccggca cggtgcagag gtgcgtccgg gggcccgagc cgtccagggg
      301 cacggcgaag acttcaacgg tcccctcttc gacgaagcag acggaatccg cggagtccaa
      361 ggatagggga gcgttgccgg cgagggcgag cggctcggct tcccgggcga gcacggtcgc
      421 caggtcgagg aggggcgctc ggacatcccg cagggtcatc gcgtcctcat tccgtgctga
      481 tgagccggga gtaggagcct tcgaccccct tgagctcatc gtgggtgccg cgctggacca
      541 ccttgccctt gtcgaggacg atgatctcgt cggcgtcccg gatggtgctc aggcggtggg
      601 cgatgatcag gcaggtgcag ccgcggcgcc ggaggttctc gtcgatggcc gcctcggtca
      661 acgggtcgag ggcgctggtc gcctcgtcca gcaccaggag ggtcggccgg cccaccagcg
      721 cacgggcgat ctccagccgc tgccgttggc cgccgctcca gttggcgccc ccctcctcga
      781 cggggctgtt atagccgccg ggccgcgccg cgatctcctc gtggatgcag gcgtccttgg
      841 ccgcggccac cacctccggc agggggaggg tcgagtccca cagcgtcagg ttctcccgca
      901 ccgtcccctc aaagaggaag acgttctggt ccaccacggc catggagttg gctacgacgg
      961 tcccgggcag atcggcgcgt ggccggccat ccaggaggat ctcgccggac caggggcgat
     1021 agaggccggt caccagcttg gcgagcgtcg acttgccgct ccccgagccc cccaccaggg
     1081 cgacccggga gccgggcctc agcagcaggt cgaagtcttc gatgagcggc gggtcgagcc
     1141 ggctgtagcc gaaggagacg ccgcgcagct ccaggtggcc ggtgagcttg accggcgcct
     1201 cggccagcag cctggggtcc tcgcccgcct tgacgctgtc gtccagccgg tagcgcagga
     1261 cgtcgtccag ccggttcatg tccccttcga ccatctggag gcgcccgccc aggctcacca
     1321 gacggttcac cggcgtcacg aagctcacca tgaggagctg gaaggcgacg aggccgccca
     1381 gggtgaggtc gccgttcatc acccgcaggc cgccgatccc caggatgagg gcggtgttga
     1441 ccgcggtcag catcgggggc acggcgtcga ggagctgggt gtagcgctcc agctcctgcc
     1501 ggccgttgac caccttggcc tgatagccgg cccagcgggt gaagaggtcc gactcgcccc
     1561 ccgttgcctt caacgtctcg atggtctgga gcccaccgat ggccgtgccc accagcttcc
     1621 cctggtcctg gaccagccgc cggttgccgt ccacccgttt gcgggagacg aagcgcaggg
     1681 ccaccacgtt gagggagacg acggcgatcc ccaccagggt cagcaccgga tcgtactgga
     1741 cgaggaccac ggcgaagaag gcgatcatca gcgcgcccag cgcgttggtc gccaggtccc
     1801 gggagaggag ctgggccacg cggtcgttga tcgccacccg cgagctgatg tcgccagcga
     1861 agcgttggct gaagaactcc gccggcaggc gcagcacgtg ccagaggaag cggctcgagc
     1921 ccgccaccgc catccgggtc tccagacgaa ggagataggt ctgctgcagc caggtgagga
     1981 cgcccgtcac cagcgccgcg gcccccatcc ccaggaggag cggcggcagc cagtcgcggc
     2041 gcccttcgag gaggacaccg tcgacgaaca ccttggagaa ggaggggatc accaggccgg
     2101 ggatcaccag cgccaggccg gcgaggatga cgaacgccag cgccgccgcc gccccggtca
     2161 ggcgccgccg cagcgccggg accagccgcg gcgcctcgcc gctcttctcg aacgacgcgc
     2221 ccggcttgaa ggtcagcacc acgccggtga aggcctggtc cagctcctcc tcggtgacca
     2281 cccgcgggcc catgcccggg tcgttgatcc gtacctgccc cttgtggaag ccgtccagca
     2341 ccacgaagtg gttgaagttc cagtgcagga tcatcggcgg ccggtgcttg cgcaggctct
     2401 gcggctcctg cttgtatccc ttggcctcaa ggccgtactg gcgggccgcc ttgacgatgt
     2461 tcgaggcctt gctgccgtct cgcgacacgc cgcaggcgat gcgcagctct tcgagcggca
     2521 cccagcggcc atggtgggcg agaacgatgg cgagcgccgc ggcgccgcac tcgaccgcct
     2581 ccatctgaag gacggtgggg ctgcgcaccg cctcgggagg cgcggcctcc ccggcaccct
     2641 ccttcttctt ccacgagagg aggcgcgaca ggctcggcat cagagcccca gcttctcccg
     2701 cagggtgggc aggacaaggg cggccggcct ctcccggcgc acgatcacgc tgccggcggc
     2761 gagggtaccg ctgctgatcc gcaggttggg tccggtggag gaggaccaac ggtagccagt
     2821 gggggtcttc gtgtcctggt ccagcgtgat gttgacctgg atgggggggc cctcggccat
     2881 cagcttctgg accagggcct cgttgccgag caggcgcacc atgccccgct ggctggcggg
     2941 gaactccccg gccgagacca ccttcccctg gatgaagccg tactcctcgc gcttgacggt
     3001 ggacggggtg atccgcgccg gcatgccgcg ctgcaccttc ttgcccgccg aggcagggac
     3061 gaagagcacc gccatcagat cctccgcggg aaactccagg ctgaggagcg gggtgccagg
     3121 attgaccacg tcgccccgat ccaccagcag ctccaccaca cggccggcgc gcggagcgac
     3181 gacgcgggcg gactcttcca gacgggcctg gcgctcccgc agctccagct ccaggtcgcg
     3241 gatctgcgcc tgccgaacct cgatctgctg gtccacctgc tgctcagctt ccaggcgcga
     3301 cagctccagg ccgttgagct cgagccgggc ttgggcgagc tggtcctggg cgctgttcag
     3361 gcgctgctcg gaggtgacga gggtctgccg ggtgacgagg ccgtctttga gcagctcctg
     3421 ctcggcctgg agctggtccc gcgccagctc cacgtctctc t
//
LOCUS       DASPJI010000349         3283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_365457, whole genome shotgun sequence.
ACCESSION   DASPJI010000349 DASPJI010000000
VERSION     DASPJI010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365457"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /gene="hemG"
                     /locus_tag="VEL74_09150"
     CDS             <1..839
                     /gene="hemG"
                     /locus_tag="VEL74_09150"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HYG62736.1"
                     /translation="AVPRIHALEREHGSLIRGAFAAGRQRKGPAPRGAMISFRQGLEE
                     LPRRLAREIGDVRTGVACERLVPTASGLRAETSAGAFEAARAVLAVPADVAARLLDEA
                     TGGRSRPLAEILYAPVAVVSLGYRRQDVAHPLGGFGFLAPRKESLRVLGCLFPSEIFA
                     GRAPEGHVALSAFLGGRTAPEIVGWDEDRIAAVVDEDLRRSLGVRGEPVFRLIRRWPR
                     AIPQYELGHGRYLDLARELETALPGLRLGGNYLSGISVPDCIQNGTALAEELLRGEGP
                     GW"
     gene            complement(841..1188)
                     /locus_tag="VEL74_09155"
     CDS             complement(841..1188)
                     /locus_tag="VEL74_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018400976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HYG62737.1"
                     /translation="MKGERSYLDYLEDILDAIAKAGEFVEGMSLEEFSEDDKTVFAVI
                     RALEIVGEAAKKIPLDLRAQANDVPWREISGMRDKLTHDYFGVNLLVVWKTVEKDLPV
                     LEYAIRELLAKSS"
     gene            complement(1185..1487)
                     /locus_tag="VEL74_09160"
     CDS             complement(1185..1487)
                     /locus_tag="VEL74_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYG62738.1"
                     /translation="MRQAGDLDQHLVKLRQHMPELEKRFRVRSLGVFGSYVHRQQKPG
                     SDLDLLVSFEELPSLFGFLALENYLSDLLGLKVDLVMADALKPRIGARILREVVPV"
     gene            complement(1532..2092)
                     /locus_tag="VEL74_09165"
     CDS             complement(1532..2092)
                     /locus_tag="VEL74_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62739.1"
                     /translation="MTDFTKEAGAETPENAFAADFEQFLGEEGEREAKVQALRAEKVE
                     DWLVPWDIEPTDDGRWAIVPPGETLAGGAEVAAYCDDPREAELYTVLVPVSRREPRFT
                     LGAEQTSEYGYPIYEKGEVVGHVREHDEELVMRMNMATALVSSPKALSLILERAGGTK
                     LEQAVEYSFAEALQLEKQKREREEEE"
     gene            2280..>3283
                     /locus_tag="VEL74_09170"
     CDS             2280..>3283
                     /locus_tag="VEL74_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYG62740.1"
                     /translation="MEASAFNDPALTIAIALAAGMLAQVAARHLRMPGIVLLLATGVL
                     LGPDLLGVIRPGTLGTSLQDLVGFAVAVILFEGGMNLNLGRLRREARSIRQLVTLGAL
                     VTAAGGAVAARLLMGWEWPTAVLFGTLVMVTGPTVITPLLRRLKIKTRVATVLEAEGV
                     LADAIGAVAAVVALEVVISPAGSLAHGAWDLVARLGFGALLGVLGGVAAAALLRFEKV
                     VPEGLESVLTLSIAVALFQGSNAILGETGIVAVVVAGMVVGNAHSHFLAEMREFKEQV
                     SVMLIGLLFVLLAADVRVSQVVSLGWAGVATVLALMLVVRPLNILVGTLGSDLTARER
                     L"
BASE COUNT          440 a         1181 c         1114 g          548 t
ORIGIN      
        1 gggcggtgcc caggatccac gcgctggagc gggagcacgg cagcctcatc cggggcgcct
       61 tcgctgccgg cagacagcgc aaggggccgg cgccccgggg cgccatgatc tccttccggc
      121 aggggctcga ggagcttccc cgccggctcg cgcgggagat cggggacgtg cggaccggcg
      181 tcgcctgcga gcggctggtg cccaccgcct cggggctccg cgccgagacc tccgcgggcg
      241 ccttcgaggc ggcccgcgcc gtcctcgccg tgccggcgga cgtggcggcg cgcctcctgg
      301 acgaagcgac cgggggccgg agccggcccc tggccgaaat cctctacgcg ccggtggcgg
      361 tggtgtccct gggctatcgc cgccaggacg tagcccaccc gctgggcggc ttcggtttcc
      421 tggcgccgcg caaggagagc ctgcgggtgc tgggctgcct cttcccctcg gagatcttcg
      481 ccggccgggc ccccgaaggg cacgtcgccc tctccgcctt cctcggcggc cgcaccgcgc
      541 ccgagatcgt gggctgggac gaggaccgga tcgccgccgt cgtggacgag gatctccgcc
      601 gctccctggg tgtcaggggg gagccggtct tccggctcat ccgccgctgg ccgcgcgcca
      661 tcccccagta cgagctgggg cacggccgct acctcgatct ggcccgggag ctggagaccg
      721 cgctccccgg cctgcgcctg ggaggcaact acctgagcgg catctcggtc ccggactgca
      781 tccagaacgg gaccgcgttg gcggaggagc tgctgagggg agaggggccg gggtggtagc
      841 tcaggagctc ttggccagca actcgcggat cgcgtactcc agaaccggga gatctttctc
      901 tacagtcttc cagaccacca ggagattcac tccgaaataa tcgtgcgtga gcttgtctcg
      961 catgcccgag atctcgcgcc acgggacatc gttcgcttgc gctctcaggt ccagggggat
     1021 cttcttcgcg gcttcgccga cgatctcgag ggcgcggatg acggcgaaca ccgtcttgtc
     1081 gtcctccgag aactcctcca aggacatccc ctcgacgaac tctcccgcct tggctatagc
     1141 gtccaggatg tcttcgaggt agtccaggta cgaccgttcg cccttcaaac cgggaccacc
     1201 tcgcgcagaa tccgcgctcc gatgcggggc ttgagtgcgt ccgccatcac caggtctacc
     1261 ttcaggccca gcagatcgct caagtagttc tccagggcaa ggaagccaaa gaggctcggc
     1321 agctcctcga agctcaccag gaggtcgagg tcactccccg gcttctgttg gcgatggaca
     1381 taagacccga agacccccag cgatctcacg cgaaaccgct tctccagctc cggcatgtgc
     1441 tgccgaagct tcaccagatg ctgatcgagg tcgcctgcct gtcgcatttc agggattcta
     1501 gccgtgccgc gtgcgcgatg cgcaccctgc cctactcctc ctcttccctc tcccgcttct
     1561 gcttctccaa ctgcaaggcc tcggcgaagg aatactcgac ggcctgctcg agcttggtgc
     1621 cgccggcccg ctccaggatc agggacaggg ccttggggga gctgaccagg gcggtggcca
     1681 tgttcatgcg catcaccagc tcctcgtcgt gctcgcggac gtggccgacg acctccccct
     1741 tctcatagat cggatagccg tactcgctgg tctgctcggc gcccagggtg aagcgcggct
     1801 cgcgccggct caccggaacg aggacggtgt acagctccgc ctcccgcggg tcgtcacaat
     1861 aggccgcgac ctcggcgcca ccggccagcg tctcgcccgg cggcacgatc gcccagcggc
     1921 cgtcgtccgt cggctcgatg tcccagggga ccagccagtc ctccaccttc tcggccctga
     1981 gggcctggac cttggcctcc cgctccccct cctcgcccag gaactgctcg aaatcggcgg
     2041 cgaacgcgtt ctccggcgtc tcggcacccg cctctttggt gaaatcggtc atcgacaagt
     2101 ctcctcgcct gctctgtttc tgccgaggag tctagagaac atcaacgcgc gaagtcaatg
     2161 cccgatcgcg ggaggaggag atctgtcgcc ggacgccggg accctgacgg cgctcccgcc
     2221 agactggccc ggtggaagcc accggatatg atgcccatgg accgcgtcgt gggagagcca
     2281 tggaagcatc cgcgttcaac gaccccgctc tgaccatcgc catcgccctc gccgccggga
     2341 tgctcgccca ggtggccgcc cggcacctgc ggatgccggg catcgtgctg ctcctcgcca
     2401 ccggcgtgct cctcggcccc gacctcctgg gggtgatccg gcccggcacc ctgggcacgt
     2461 cgctccagga cctggtcggg ttcgccgtcg cggtgatcct cttcgaaggg ggcatgaacc
     2521 tcaacctcgg gcggctccgc cgcgaggccc ggagcatccg ccagctcgtc accctcggcg
     2581 cgctggtgac cgcggccggc ggcgcggtgg cggcgcggct cctgatgggt tgggagtggc
     2641 ccaccgcggt cctcttcggg acgctggtga tggtgaccgg gcccaccgtg atcacaccgc
     2701 tgctgcggcg cctcaagatc aagacccgcg tcgccacggt gctcgaagcc gagggggtgc
     2761 tggcggacgc catcggcgcc gtcgcggcgg tggtggcgct ggaggtggtc atcagccccg
     2821 ctgggtcgct ggcgcacggc gcctgggacc tggtggcgcg cctcggcttc ggcgccctcc
     2881 tgggggtcct cggcggggtc gcggcggcgg ccctcctccg cttcgagaag gtggtgcccg
     2941 aggggctgga gagcgtgctg accctgtcca tcgcggtcgc cctcttccag gggagcaacg
     3001 ccatcctcgg cgagaccggg atcgtcgccg tggtcgtggc cggcatggtg gtgggcaacg
     3061 cccactccca cttcctggcg gagatgcggg agttcaagga gcaggtgagc gtgatgctca
     3121 tcggccttct cttcgtgctc ctcgccgccg acgtccgggt gtcccaggtc gtctccctgg
     3181 gatgggcggg ggtggcgacg gtcctcgccc tgatgctcgt ggtccgcccg ctgaacatcc
     3241 tggtgggcac cctggggtcc gacctgacgg cgcgcgagcg gct
//
LOCUS       DASPJI010000350        28558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_484610, whole genome shotgun sequence.
ACCESSION   DASPJI010000350 DASPJI010000000
VERSION     DASPJI010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 28558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28558)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28558
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484610"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..284
                     /locus_tag="VEL74_09175"
     tRNA            212..284
                     /locus_tag="VEL74_09175"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:245..247,aa:Ile,seq:cat)
     gene            432..1187
                     /locus_tag="VEL74_09180"
     CDS             432..1187
                     /locus_tag="VEL74_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HYG62741.1"
                     /translation="MNQHLEAIVELQSAIDHLREADKRLHGIPDWMRELHEEHAARKA
                     EIDKLNETAEEATRERRTAEAAVADAQEKLKKYQQQINRVTTQREYGALLQEIDTVKT
                     QITASEEQALSALERNEQAKRDLDAKMESFRELDERYATELARWEAEKPGVAEQADGL
                     RARIKTLRESLPRPVVSQFDRILDRHPGNALAPVRPIERPGRAMREWHCVACNYRVRP
                     QTVVEIRNGGALVQCDSCKRILYIEPEETEAAS"
     gene            1209..1976
                     /locus_tag="VEL74_09185"
     CDS             1209..1976
                     /locus_tag="VEL74_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HYG62742.1"
                     /translation="MTEADARGGVAERLARIRERIDAACRAAGRAADGVVLVGVSKTQ
                     PAEALAEAWRAGLHVFGENRVQEALSKSRGLAPLLAASSGDGARSPDWHLIGPLQSNK
                     VRAALGLFSTVHSVDRPGIAEALDREAASRGLTVTGFLEVNLGGEESKHGFPAEGFAE
                     AVRPLADLRHLRIAGLMAIPPMGDDPEESRPWFRRLRALRDGLAARPEWHGFPGWLSM
                     GMSDDFEVAIQEGATHVRVGTALFGPRQPRPSGPSNE"
     gene            2069..2566
                     /locus_tag="VEL74_09190"
     CDS             2069..2566
                     /locus_tag="VEL74_09190"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HYG62743.1"
                     /translation="MALTPLEIQKMRFTQKMRGYDPAEVESFLSLMAEELTTRLAQIE
                     KLERENRWFRQRLDEAEQREHQLQQTLLRAQKVSDEITGNARREAELTVKEAELAADK
                     IVQQALEQAARIESKINELRIQRREVHLKFKNTLDLFQSILEADVAEERGPANVVTLS
                     RKRQA"
     gene            2587..2892
                     /locus_tag="VEL74_09195"
     CDS             2587..2892
                     /locus_tag="VEL74_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62744.1"
                     /translation="MVDIPPGTSRLFPDHDPGDLAGGDPSFVIARLLEDGDSADLAWL
                     VRTLPETALAAWLERRGARGLSLRSRAFWEVVLGRPAGPAGGSAPEVEAVRRAVWPL"
     gene            2880..3572
                     /locus_tag="VEL74_09200"
     CDS             2880..3572
                     /locus_tag="VEL74_09200"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HYG62745.1"
                     /translation="MAALTPRHPGALSPHAQALLGRLAERPWSDDFYLAGSAALALYT
                     GHRPVRDLDLMAGTNRLTSPERRDLLGDLLALEPATEVETARDGYLYVRVAGEAIQGT
                     GIRFFYYPYPLADPFQEFAGFPVISAVDLGLMKLGAIISRGTRRDFVDLYMLCRELPL
                     AGLLERADDKFGHVRDFPLQALKGLADLSVAAEDPMPCLALPLEWEEVESWVLGQVRD
                     LGRSRFGMEALP"
     gene            3569..4843
                     /gene="hutI"
                     /locus_tag="VEL74_09205"
     CDS             3569..4843
                     /gene="hutI"
                     /locus_tag="VEL74_09205"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HYG62746.1"
                     /translation="MSSGVPVPDLLIENLAEVATPEGSTPRRGADQGRLRRLRDAEVL
                     CRDGRIAFAGPSEERRRLFGELADTPRLDGRRGTLVPGFVDPHTHLPWAGSREEEFAA
                     RLAGKTYQEIAAAGGGILSTVAATRGAGEEELAAAVLRRLDLMLAHGTTTAEAKSGYG
                     LSLESELRQLQAIRRAAAAHPVELVPTLLAAHEVPPEWRHDRGGYVDLVCNEIVPAAA
                     EQGLARFCDVFCERGVFSAEESRRVLEAGARHGLAPRLHADEFEDSGGAELAAELGAL
                     SADHLMAVSPAGIDALARSAVTAVLLPGTSFFLMKHRYAPARRLVEAGVPVALATDCN
                     PGSSYTESMPMILTLAVFQLGLSIEESLTAATLNSACSLGLGAEIGSIEVGKRADLLL
                     LDAPNLLHLAYHYGVNPVAAVVKGGRVVRRAV"
     gene            5008..5379
                     /locus_tag="VEL74_09210"
     CDS             5008..5379
                     /locus_tag="VEL74_09210"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HYG62747.1"
                     /translation="MITKSGLRYVDLKVGEGEEAKPGKVVEVQYTGWLQNGDGTPFET
                     SRDCDEPLTFRVGAGDVLKGWDEGIQGMKVGGKRKLVVPPELGYGRQGAGSVVPPNAT
                     LVFEFELLAVRGAPAPQGKGR"
     gene            5379..5732
                     /locus_tag="VEL74_09215"
                     /pseudo
     CDS             5379..5732
                     /locus_tag="VEL74_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015132140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
     gene            5868..7646
                     /locus_tag="VEL74_09220"
     CDS             5868..7646
                     /locus_tag="VEL74_09220"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HYG62748.1"
                     /translation="MSERVDEVAEQGTESRGQVDSPGLSRRRFLGLGGAAAVAATSGL
                     AGFEAPAHAVEAPDLGLAAEAATLTPAARARRNACMKMRKDMAEAWWKKGMPDHPVNG
                     DEDRYPNKIASYSKSMPHNEFGEVDLDAYESLLRAVRSGKAADYDAMILHPGARRQTS
                     PQCGLAFDMEGVDSHGVAVPAPPRFDSREQAAEIVEHYWMSMLRDVSFLDYGGSPLVE
                     EALADLNRFGADYKVPKRNGRATAQTLFRDIAPGTAAGPYISQFMWLPTPFGVEYVER
                     KMWTLSPGSDHMQRFDEWLAVQNGLVPGKESFLGARRYIINGRDLSAWVHVDVLFQAY
                     FNACLILMTPPDGSDVGGGLGCPFNPGNPYLGNATQDGFCTFGPPAAKSLLCEVASRC
                     LKATWHKKWQVHRRLRPEAYAGRLHVHLNESPGRYEGAFHPDLFGARALDRIESFNGG
                     GHLLPQAFPEGSPTHPSYTAGHATVAGACVTILKALFDTVGRLIASPVVPNADGSALV
                     PYTGPTLTVEGELNKLASNIASGRNVAGVHWRTDSHASLRIGQEVAISLLEEQTLIHN
                     EMSPSFHFRGFDGETIVVRDGGVFDS"
     gene            7721..7996
                     /locus_tag="VEL74_09225"
     CDS             7721..7996
                     /locus_tag="VEL74_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HYG62749.1"
                     /translation="MSADRSAKRWPVQDAKARFSEFLEASLNEGPQIVTRRGVETAVL
                     VPFEEWRRLQSSARPTLKELLLAEESRAEIPVPSRHRWRRRTPEALE"
     gene            8003..8410
                     /locus_tag="VEL74_09230"
     CDS             8003..8410
                     /locus_tag="VEL74_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG62750.1"
                     /translation="MYLLDTNVISELRRPRPHGAVVAWLEGVADHDLHLSAVTLGELQ
                     AGVEITRDQDPGKASQIEDWIDQVAQTWNVLPLDGRTFRVWAKLMHRRSDDLLADALI
                     AATAMVHHLVVVTRNVRDFAPLGVETLDPFKAG"
     gene            8701..9075
                     /locus_tag="VEL74_09235"
     CDS             8701..9075
                     /locus_tag="VEL74_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62751.1"
                     /translation="MQSDDDRNTPRLPNIFLREFLDLLGRRNEPPAAAEAAVAGPWHI
                     EEVHATFYAVYRSGESVARGHRPLAILTDPNHALLIAALLPATGRDPLYRLHKDRTPS
                     GYAIEAAGGEVIGPWPSSTTSS"
     gene            complement(9346..10980)
                     /locus_tag="VEL74_09240"
                     /pseudo
     CDS             complement(9346..10980)
                     /locus_tag="VEL74_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
     gene            11751..12140
                     /locus_tag="VEL74_09245"
     CDS             11751..12140
                     /locus_tag="VEL74_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYG62752.1"
                     /translation="MTRAIISPADLSQRTQEILERVREGELAIVSSEGEERIVLLDAL
                     DYRLLRALALSATGTGADGPGELLDARSLRDYLDEKINLGKAAELLHLSRFELEESFQ
                     RLGLPLQRGPRSIEDAQAEISAARRHA"
     gene            13384..13659
                     /locus_tag="VEL74_09250"
     CDS             13384..13659
                     /locus_tag="VEL74_09250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYG62753.1"
                     /translation="MSQHTVGIRQLKEQLSHYVEQVKTGDTVLITEKGKPVGRIVPVA
                     PSIRERLEQLAEANLPAWSGKKLGPQAPVVPSGGAKTVADLLLEDRE"
     gene            13978..14808
                     /locus_tag="VEL74_09255"
     CDS             13978..14808
                     /locus_tag="VEL74_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62754.1"
                     /translation="MRLRTMSFLLMLSLLLGFSPVSADPVIYRGIDVFATAANGKTFY
                     DFAQRPIPAGFFCKGSKPFAGRVAFKGLPLATEVPGQLWGADTVVERLDDAVFDEKGV
                     ATTRLQFRALSLVSIAPIKTGCGAFDVYLSLGGKQRVTTMSIHRTQERGGEFVGPLAV
                     DVRMRFVPVKPARGKGSPRKLELPGSITFPGAPMPWSLSSSPLAKRIDLVVVDTDGDQ
                     APDSLLPGTSNFAPGQSPDSTWSNKGLCSCCPGEQCHADDGEMHCFTVTMCPNTHSCC
                     "
     gene            complement(14859..16589)
                     /locus_tag="VEL74_09260"
     CDS             complement(14859..16589)
                     /locus_tag="VEL74_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62755.1"
                     /translation="MRLMILCILCLGSLATLACTGGEDASAGAPANGEAASAGSGSTA
                     PAGAEGKIVPDIASRVAKFAPIPLQADLSALSASDRQVLDLLIQASHYMDDIFLRQAW
                     AGNPEMQREIAGWQGENAEAAREYFRINFGPWDRLAESEPFIGDLKHPEGAGYYPEDM
                     TKEEFEAWIAKNPGDKERFTSNFTVIRRGPDKELTAVPYSQEYKAWLEPAAKLLRQAA
                     GITDNASLKRYLTTRADAFLSDDYYESDVAWMDLDAPVEVTIGPYETYEDGLFGYKAA
                     FESFVTVNLPKESAALARYKERLPWLEGNLPIPDEHKNKNRGTESPIRVVDTVFTAGD
                     TRAGVQTIAFNLPNDERVREAKGSKKVLLRNTMRAKYDKILVPIAERVIAKDQVADLT
                     FEAYFDEVLHHELSHGLGPGSITVNGRKTEARLELKELYATLEEAKADVMGMYNILAL
                     IDRKEMPAELRKSLEPTYVAGLFRSARFGVDEAHGQGVVAQFNYLIAKGALEVDQEGR
                     FRAVSAKFPGALRELLAEMLMLQARGDYAGTKKFLDTYGKAGDNLRNAIAKLSDVPVD
                     IRPIYQADRK"
     gene            16698..17156
                     /locus_tag="VEL74_09265"
     CDS             16698..17156
                     /locus_tag="VEL74_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HYG62756.1"
                     /translation="MADRVKPYRASVKYWLLSTDPEDYSYDDLDADGSTVWDGITDYV
                     DLKNLRDMDKGDLALIFETGAEPAVVGLATIASDPYPDPEQGDATVYVVDVEPEQWFE
                     EPVRLADFQDDPAFQELDFLNAPERGVVSVPEPLWNRILEMGRVPAEERV"
     gene            17153..17635
                     /locus_tag="VEL74_09270"
     CDS             17153..17635
                     /locus_tag="VEL74_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62757.1"
                     /translation="MRRAATAVVLAVLAAGAAVGPAAAQDIAFNRWGVRAGASDDPDQ
                     VVVGAQADFGTFVRNVRFQPHADLGLGDDVTIFSLTAPAHYLLPVEGGFTLYGGGGLT
                     LAWIDSDRPEDRDDSDFEITPMVAGGAEWPAGPGLLSVELNLSGGDLPGVKLVAGWMF
                     "
     gene            complement(17641..18303)
                     /locus_tag="VEL74_09275"
     CDS             complement(17641..18303)
                     /locus_tag="VEL74_09275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HYG62758.1"
                     /translation="MAQRSSMAGRLPAALLSLLLPGLALSCQSSGPAQAPGIGGLLDG
                     PTRWLMLPEEQRQARQLTSTRDAALFTETFWDRRDPDPATPANEYARTFYERVEAADK
                     LYSEDGTRGSMTDKGRAFILLGSPPVLRYSQKQVPAWDPGRPGTRPSVEARGIAVESW
                     IYQLDQLDPRLAELIRQEDGEETLEVVVTFAVEPHRTYLLEGEEYLELAILTARQDAP
                     DH"
     gene            complement(18319..18600)
                     /locus_tag="VEL74_09280"
     CDS             complement(18319..18600)
                     /locus_tag="VEL74_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HYG62759.1"
                     /translation="MAEPGEGLNVKIDDDELKGRYSNLLRIAHTREEFILDFINLMPP
                     QGVVTARIITSPGHMKRIIRALSMNLERYEQTFGPIHEAPEPGGDQGVH"
     gene            complement(18658..19953)
                     /locus_tag="VEL74_09285"
     CDS             complement(18658..19953)
                     /locus_tag="VEL74_09285"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HYG62760.1"
                     /translation="MEIQDFARRLAAVAVAAADPEKLVREELTRRLGQMSGARFGAIL
                     ALGKAAAGLARGTRGVLSSPARRLLIRPHSSPALLDPGWEQLAGGHPLPDRQSLAAGD
                     RAVRWLGQLAPDQALLALLSGGASACVEVPAPGITLEDLAATQRALLASGLPIQAVNA
                     VRKHLSSFKGGGALRTASAGRVLALLLSDVPGDDPSTLASGPFAADPTTFAEALAAVE
                     GVGLPEAVRRHLEAGARGVVPETVKPGDPALVRVETVVVGGNGTAVAAVLAEARRNGF
                     TAVDGPLEGEAARTARVLVARGRALGHGENGVALVLGGETTVTLTGGTGRGGRNGELA
                     LAAARELAAAPPEAGAREVVLALATDGEDGPTRWAGAVVDGGTWEAVRRAGKDPRAAL
                     ERHDSYGALSAVPGSLLETGPTGTNVADLAVYLRMPDAP"
     gene            complement(19969..21345)
                     /locus_tag="VEL74_09290"
     CDS             complement(19969..21345)
                     /locus_tag="VEL74_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG62761.1"
                     /translation="MSEPEASLSPATPAAPLGADEAIRVEGLSKLYRRTMPGDRLRTL
                     KSALVGGSLTRDLKREEAIAALEGVDFTVAPGEAFGVIGGNGSGKSTLLKLVAGMLKP
                     TTGRITVRGRVAALIELGAGFHPEISGRENVFINGAVLGLSRKLIESRYDEIVEFSGL
                     GDFMEEPVKNYSSGMYVRLGFAVAVHTDPEVLLVDEVLAVGDEAFAHRCIRRIEEFLA
                     AGKTLLLVSHSLDLVENVCDRVLWLDNGVQRLVGTPRRVIDAYRQAVAEGEGEANRAA
                     KQQRDEAAAEQPPAAEPPAGGASGSGAAAQGEAEPLRWGSRDAEITGVRLLVGGTERY
                     HLETGEEAVFEIRARADRPLCDFVFGVAVSTPRGFECWGTNTDLAGFVPGELAGETTV
                     RLTCPALRFAPGDYLVDVAVHARDGAPYDYQRKALAFTVSSPQRGVGVYFPEHRWEFS
                     GGVRWEEE"
     gene            complement(21342..22145)
                     /locus_tag="VEL74_09295"
     CDS             complement(21342..22145)
                     /locus_tag="VEL74_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG62762.1"
                     /translation="MFRVASQIVRFRGLLATLTSRELKARYRGSVLGFLWSLANPLLL
                     LAVYTFVFSVVFQSRVPGIEPYALFVVCGLFPWIWLSTSTLEGSMSLIANSGLIRKAV
                     FPAELLPMVTVLSNFVHLLFALPIVAVALFAGWLLQYPVGGSGAVLLPAILLLHLPMV
                     AGIALGAAALTVHFKDVRDILQNLLTLMFFLTPILYPLDSVRAFPLIWWIVRLNPFTP
                     FTLAYQEVLFRGRAPEPDLWIQMALVSLLAWVVGAGLFERLRETLVEAA"
     gene            22255..24519
                     /locus_tag="VEL74_09300"
     CDS             22255..24519
                     /locus_tag="VEL74_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62763.1"
                     /translation="MPEWSPVDLLPGLWAGALLLVLAAALRRWYDPVPAPVLAAFGAA
                     VALLFGGVLFGGGILLPLDNLRGHVPFQGLAPAEPHGNILQGDLIELVAPSQAAVGEA
                     LGERRWPLWNPRVGAGMALISDPQAQPFQPLVLLAAPMPLERAPGVTAALRVLVALTF
                     SFLWMRRQGLGAVPALAGAFAYGLGGFLLLWVGWPLANSAALLPLVLYALTLCGQRGL
                     RRDAALLAGGLLALLLAGHPETILFGLALAAVFLLDQVRRRPPGTRRPLLRTAALAAV
                     AAALAAAPVLLPVAHDVPRSMRAARVSDPAVHSSPAGGLAPRWLPIAAPNAYGNSRFI
                     HYWGLSNTNEDAAGFIGTAALLAALLSLGARRRFPQEGTALGIAGLCLVGLALPAFVP
                     LPDSLASRRVLLPFVLCLAYLAACALERFRQGEVRRRAVLAAAAVLAGVVVWGYVTHP
                     DPSDPERLAVFRYGWLLWQMRFLVLGALLLALPGRGEGNRWVRTAAAGGLALLVAVEL
                     VLIHRPANPPMPRRLALPVTPPLELLRESLAEDRAGLTGLRMAALGRALPPNLASLYG
                     LPDARVYNPMAPQAYVEVTAPITAAWWGELPEFGAPDHPLYPRLGVRYLMTEPGERLP
                     APWRLTLEDAGAWIWEHPRALPRLFLEGRGRGALRITRLKSAWITARVRPREGGLLDS
                     SLYQDGGWRLLVDRRLRPGHPNGGPFVVAELPEGARRVDLVYRPRAFLLGCLLAPLGL
                     ALAAALSVPPPGRR"
     gene            24529..24837
                     /locus_tag="VEL74_09305"
     CDS             24529..24837
                     /locus_tag="VEL74_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62764.1"
                     /translation="MSETRKLTIRCPDCGSDLVVDAATGQVLSHRKPKQPLAGGKDFD
                     ALLRGLDEDKAKAEDIFQREFEAMKDRDRIMEEKFREAMKRAEEDPDEGPPRRPFDLD
                     "
     gene            25054..26733
                     /locus_tag="VEL74_09310"
     CDS             25054..26733
                     /locus_tag="VEL74_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HYG62765.1"
                     /translation="MRPTAEGAAKDDNREQDKSPSTPPPPAEAPQPAAPEPAEAPVEA
                     RADEARTAEARQPETWPADPRVEEPHTGVTGHGGTSEAGEPPLPWETPRESPAELAGE
                     PDTPELLPGVPEAVELSLEAVEPLPVGYTAEDGDDDSEDDEIPGAVAETAEGEGAERS
                     EEGPARKKRSRRSRGGRRRNGRDAATDSAEAADDEDEALAEEPTPITVPPPPPPSIPA
                     RTAREGREREGREGREGREGRERDRGDREPREPRESRESRESYSFDAQPPFQPVRGRD
                     SYVFAPSDMEGGFGGGPFGGREIVISVPERQRAGSDERKIALFCDLENIALGVRDSEI
                     SKFDIDLVLERLLEKGKIIVKKAYADWERYSEYKRPFHEAAIELIDIPQKYYSGKNSA
                     DIKMVVDAMDLSYSKEHLDTFVMLSGDSDFSPLVSKLKENNKYVIGVGVKNSSSNLLI
                     DNCDEFLYYEDVWRDAQKGPKLDGLNKKSAEVFSLMVDAIQALLRENKDVLWGSMIKQ
                     TMQRKKPSFSESYYGYTTFSELLEDAERKQIIKLKKDQRSGTYIVTGFAKR"
     gene            complement(26744..28267)
                     /locus_tag="VEL74_09315"
     CDS             complement(26744..28267)
                     /locus_tag="VEL74_09315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG62766.1"
                     /translation="MRHLLREGDFFHLFAENLKDYAVFLLDTEGRVATWNVAAERILG
                     YREDEILGRPSACFFTPEQLEHDYPRRELEMAAATGKAGDDRWQVRKDGSWFFANGVT
                     VALCDEAGTLQGFAKILRDYTERKRLDEELQSRAEALARADRAKDEFLAVLAHELRNP
                     MAPIFNALHLLDRHPGDERTERQAKSIIERQVRQLARLIDDLLDVSRITLNKIELRKE
                     RVELHRIVENAVQTAYPLIDARRHELTQSLPAETVWLEGDATRLEQVVANLLNNAAKY
                     TEPGGRIHIGAERQRDQVVLRVRDTGVGIPPELLASMFDPFTQGSRTLARSQGGLGVG
                     LTLARKLLELHGGTIEAHSEGPEKGSELTVRLPVAEPGGGQSEQPSPPTKTSALRVLV
                     VDDSEDTAETMGMLLHLAGHEVRLAFDGLAAVEMATADRPDAILLDIGLPVMDGYEVA
                     RRLRQNPAMREVVLIAASGYGQETDKAQSREAGFDVHLVKPIDPQELLRLLGTIRKE"
     gene            complement(28269..>28558)
                     /locus_tag="VEL74_09320"
     CDS             complement(28269..>28558)
                     /locus_tag="VEL74_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62767.1"
                     /translation="QTTESLQADYSIVIPFLVKALLDNRARYARWAERMGEEALFEKH
                     PKARGYLRPREGYRLFERREALVSALVEDVRQNREWLLESIHYPLAVGVTP"
BASE COUNT         4367 a         9848 c         9819 g         4524 t
ORIGIN      
        1 gccggacagt ctccgatccg cggcgcacgg cggagatggc gcgccgcctc gccgccgcct
       61 ggagcacctt cgtgccgggt gagctggcgt cagggctcga gtcccgggag agcggggcgg
      121 gtgccgggga cacgaagtcc ggagagaccc cgggtctgcc tttctgaaat cggggtgata
      181 gactgcaaag ccgccctggc cagggctttc cgggtccata gctcagcggt gagagcatcc
      241 ggctcataac cggttggtcc ctggttcgaa tccaggtgga cccacttcgg gcatgcctgt
      301 acaagatctc ttctgccgcg gctcgatccc ggggcctcgt ccagcaagga agagcgggat
      361 ccggcccgct cgtgcctgcg aggacgccgc ggtggagtaa cggttcccgg ggaggagcga
      421 gaaatctctc gatgaatcaa cacttagagg ccatcgtgga gctacagagc gccatcgatc
      481 atctgcggga ggccgacaag cgcctgcatg gcatccccga ctggatgcgc gagctgcatg
      541 aagagcacgc cgccaggaag gcggagatcg acaagctgaa cgagacggcg gaggaggcga
      601 cgcgggaacg gcgcacggcc gaggccgcgg tcgccgacgc ccaggaaaag ctcaagaagt
      661 accagcagca gatcaaccgc gtcaccaccc agcgcgaata cggcgccctt ctccaggaga
      721 tcgacacggt caagacgcag atcacggcgt ccgaggagca ggccctgtcg gcgctcgagc
      781 gcaacgagca ggcgaagcgg gacctcgacg ccaagatgga gagcttccgc gagctcgacg
      841 agcgctacgc caccgagctg gcgcgctggg aggccgagaa gcccggcgtc gccgagcagg
      901 ccgacgggct gcgcgcccgc atcaagacgt tgcgcgagtc gctcccccgg ccggtggtgt
      961 cgcagttcga ccgcatcctc gaccgtcacc cggggaacgc cctggcgccg gtccgcccga
     1021 tcgagcggcc cggccgcgcc atgcgcgaat ggcactgcgt cgcctgcaac taccgggtgc
     1081 ggccgcagac cgtggtggag atccgcaacg gcggcgccct ggtgcagtgc gactcctgca
     1141 agcgcatcct ctatatagag cccgaggaga ccgaggcggc ctcctgaggc cggcgcaggg
     1201 gcgaggggat gaccgaggcc gacgcgcggg gcggggtggc ggagcgcctc gcccgtatcc
     1261 gtgagcgcat cgacgccgcc tgccgcgccg ccggccgggc ggcggacggc gtggtgctgg
     1321 tcggggtgag caagacccag ccggcggagg cgctcgccga ggcctggcgg gcagggcttc
     1381 atgtgttcgg cgagaaccgc gtgcaggagg ccctgtccaa gagccgcggc ctcgcccccc
     1441 ttctggccgc ttcgtcggga gacggcgcgc ggagtccgga ctggcacctc atcggccccc
     1501 tccagtccaa caaggtccgc gccgccctcg gcctcttctc cacggtgcac tcggtcgacc
     1561 ggccgggcat cgccgaggcg ctggaccgcg aagcggcctc ccgcgggctg accgtcaccg
     1621 gcttcctcga ggtcaacctg ggcggcgagg agtccaagca cggtttcccg gccgaggggt
     1681 tcgccgaggc ggtgcgcccc ctggccgatc tccggcacct gcggatcgcc ggcctcatgg
     1741 cgatcccccc catgggcgat gaccccgaag agtcccgtcc ctggttccgc cgcctgcgcg
     1801 cgctgcggga cggcctggcc gcccggccgg agtggcacgg cttccccggc tggctctcca
     1861 tgggcatgag cgatgacttc gaggtggcca tccaggaggg cgccacccat gtgcgggtgg
     1921 gcaccgccct cttcgggccg cggcagcccc gtccttccgg gccttccaat gaatgagcgg
     1981 gattcatgct aagatggcta tccattttcg gcacgaactt cgcgacgagg aacgccccgc
     2041 aggaggctgt tcgccaggag gctgcaccat ggctctgacc ccgctcgaga tccagaagat
     2101 gcggttcacc cagaagatga ggggatacga cccggccgag gtggagagct tcctctccct
     2161 catggccgag gagctgacga cccggcttgc ccagatcgaa aagctggagc gcgagaaccg
     2221 ctggttccgc cagcgcctgg acgaggccga gcagcgggag caccagctcc agcagacgct
     2281 gctgcgggcc cagaaggtct cggacgagat caccggcaac gcccgccgtg aggccgagct
     2341 gacggtcaag gaggccgagc tggcggccga caagatcgtg cagcaggcgc tcgagcaggc
     2401 ggcccgcatc gagagcaaga tcaacgagct gcgcatccag cgccgcgagg tgcatctcaa
     2461 gttcaagaac accctggacc tcttccagag catcctcgag gccgatgtgg ccgaggagcg
     2521 gggtccggcg aacgtcgtga ccctgtcgcg caagcggcag gcctgagatc ccctgataac
     2581 atctagatgg tggacatccc tcccggcaca tcgcggctct ttccggatca cgatccgggt
     2641 gacctcgccg ggggggatcc ctcgttcgta atcgcccgcc tgctggagga cggcgactcc
     2701 gccgacctcg cctggctcgt ccgcaccctc cccgagaccg ccctggccgc ctggctcgag
     2761 cgccgcggcg cccgcggcct gtcgctgcgc agccgggcgt tctgggaggt ggtgctcggt
     2821 cgaccggcgg ggccggcggg tggctcggcg ccggaggtgg aggctgtgag gagggcggtt
     2881 tggccgctct gaccccgcga catcccggag cgctgtcccc ccacgcccag gcccttctcg
     2941 gccggctggc ggagcgcccc tggtccgacg acttctatct cgccggctcg gccgcgctgg
     3001 cgctctacac cggccaccgg ccggtccgcg acctcgacct gatggccggg accaaccgcc
     3061 tcaccagccc cgagcgccgc gacctgctgg gcgacctcct ggctttggag cccgccaccg
     3121 aggtggagac ggcgcgcgac ggctatctct atgtccgcgt ggccggcgag gccatccagg
     3181 gtaccggcat ccgcttcttc tactatcctt acccgctggc cgacccgttc caggaattcg
     3241 ccggcttccc ggtgatctcg gcggtcgacc tcggactcat gaagctcgga gccatcatca
     3301 gccgcggcac ccgccgcgac ttcgtcgacc tctacatgct ctgccgcgag ctgccgctcg
     3361 ccgggctgct ggagcgggcg gacgacaagt tcggccacgt gcgggatttc cctctgcagg
     3421 ccttgaaggg gttggccgac ctctccgtcg cggccgagga cccgatgccc tgcctggcac
     3481 ttcccctgga gtgggaggag gtggagtcct gggtgctcgg gcaggtgcgc gacctgggcc
     3541 gctcccgctt tggaatggag gccctgccat gagctccggc gttcccgtcc ccgatctcct
     3601 gatcgagaac ctcgccgagg tggcgacccc cgaaggctcg acgccgcgcc gcggtgctga
     3661 ccaggggcgg ttgcggcgcc tgcgggacgc ggaggtgctc tgccgcgacg gccgcatcgc
     3721 cttcgccggc ccgtccgagg agcgccggcg cctgttcggc gagttggcgg acacgccgcg
     3781 cctcgacggc cggcgcggta cgctggtgcc ggggttcgtc gatcctcaca cccacctgcc
     3841 ctgggcgggg agccgcgagg aggagttcgc cgcccggctc gccggcaaga cctaccagga
     3901 gatcgccgcc gccggcggcg gcatcctctc gacggtcgcc gccacccgcg gcgccggcga
     3961 ggaggagctg gccgcggccg tgctccggcg cctcgacctg atgctcgccc acggcaccac
     4021 cacggccgag gcgaagagcg gctacggcct gtcgctggag agcgagctgc ggcagctcca
     4081 ggcgatccgc cgcgccgccg ccgcgcatcc ggtggagctg gtgccgaccc ttctcgccgc
     4141 gcacgaggtg ccccccgagt ggcggcacga ccgcggcggc tacgtcgacc tggtctgcaa
     4201 cgagatcgtg ccggcggcgg cggagcaggg gctcgcccgc ttctgcgacg tcttctgcga
     4261 gcgcggcgtc ttctccgccg aggagtcgcg ccgcgtcctc gaggcgggcg cgcgccacgg
     4321 cctcgccccc cggctgcacg ccgatgagtt cgaggactcc ggcggcgccg agctggccgc
     4381 cgagctaggc gcgctgtcgg ccgaccacct gatggctgtc tcgcccgccg gcatcgacgc
     4441 cctggcgcgc tccgccgtca ccgccgtgct cctcccgggg accagcttct tcctgatgaa
     4501 gcaccgctac gcgccggcgc ggcggctggt ggaggcgggc gtgccggtgg ccctggccac
     4561 cgactgcaat ccgggctcct cctacaccga gtccatgccc atgatcctga ccctggcggt
     4621 gttccagctc ggcctctcca tcgaggagag cctgacggcc gccaccctca attccgcctg
     4681 ctccctgggg ctgggcgccg agatcggttc catcgaggtg ggcaagcggg ccgatcttct
     4741 ccttctcgat gctcccaacc tcctccattt ggcctaccat tacggagtca acccggtcgc
     4801 cgccgtcgtc aaggggggcc gggtggtgcg acgggccgtt tgaccgtaga ataggcgacc
     4861 atcctgatca cgaatccgct ggcgtctgcc ggcaggggaa tcagcatgaa tcgcaggcat
     4921 caactctggt gtgccccgtt cgctgccttt ctggccgtag cgggcgtcct ctccgccgcg
     4981 gaggagaccc agcgccccga gcgggaagtg atcaccaagt ccgggctccg ctacgtcgac
     5041 ctcaaggtgg gggaggggga ggaggccaag ccggggaagg tggtggaggt gcagtacacc
     5101 ggctggctcc agaacggcga cggcactccc ttcgaaacca gccgcgactg tgacgagccg
     5161 ctgaccttcc gggtcggggc cggtgacgtc ctcaagggtt gggacgaagg gatccagggg
     5221 atgaaggtgg ggggcaagcg caagctggtc gtcccgcccg aactgggtta tggcaggcag
     5281 ggcgcgggca gtgtggttcc ccccaacgcg accctggtct tcgagttcga gctgctggcc
     5341 gtgcgggggg cgccggcgcc acagggaaag ggacgatgat gagcgacaac aacgaggtca
     5401 ccacgggctc cggtctccgc tacaccgaac tgcagacagg cgagggggat gaggccaagg
     5461 cgggcaagac cgtcgacgtc cactacaccg gctggctgga gaacggcacc aagttcgatt
     5521 ccagccacga ccgccgacag ccgttcagct tccatctggg cggtggccag gtcatccgcg
     5581 gctgggacga gggcgtcgcc ggcatgaaag tcggcggcaa acgccggctg gtcatcccct
     5641 ccgacctcgg ctacggcgcc cgcggcgccg gcggcgtcat tcccccgaac gccaccctga
     5701 tcttcgaagt cgagctgctc aaggtgcggt agcacctctt ctggggcccg cagggggagg
     5761 cggttcctcc cccctcgccg ggctcctgct tcgtcccgcc tctattctcg ctggcgaaca
     5821 atttctatac agtttggggg ctacccgaag cctgggggag gtcgcgcatg tccgagcgtg
     5881 tggacgaggt cgccgagcag gggacggaga gtcgagggca ggtggattca cccggtctca
     5941 gccgccgcag gttccttggt ctgggcggag ccgccgcggt cgcggcgaca tcgggcctgg
     6001 cgggcttcga agctccggcg catgccgtgg aggctccgga cctcggcctc gcggcggagg
     6061 ctgccaccct gacgcccgcc gcccgggcgc gccggaacgc ctgcatgaag atgcgcaagg
     6121 acatggccga ggcgtggtgg aagaagggca tgcccgacca tccggtcaac ggcgacgagg
     6181 accgctatcc gaacaagatc gcgtcctact cgaagagcat gccgcacaac gaattcggcg
     6241 aggtcgacct cgacgcctac gagagcctgc tcagggcggt ccgctccggc aaggcggcgg
     6301 actacgacgc catgatcctc catccgggcg cccgccggca gaccagcccg caatgcggcc
     6361 tggcgttcga catggagggg gtcgacagcc acggggtggc agtgccggcg ccgccgcggt
     6421 tcgacagccg ggagcaggcg gcggagatcg tcgagcacta ctggatgtcg atgctgcgcg
     6481 acgtgagctt cctcgactac ggcggctcgc cgctggtgga ggaggcgctc gccgacctga
     6541 accggttcgg cgccgactac aaggtcccca agaggaacgg ccgcgccacg gcccagacgc
     6601 tgttccgcga catcgcgccc ggcaccgcgg ccggacctta tatctcgcag ttcatgtggc
     6661 tccccacccc gttcggcgtg gagtacgtcg aacgcaagat gtggaccctg agccccggct
     6721 ccgaccacat gcagcgcttc gatgaatggc tggccgtgca gaacggcctg gtgccgggga
     6781 aggaatcctt cctgggagcc cggcgataca tcatcaacgg ccgcgacctc tctgcctggg
     6841 tccacgtcga cgtcctgttc caggcctact tcaacgcctg cctgatcctg atgacgccgc
     6901 cggacggctc cgacgtgggc ggcggcctcg gctgtccgtt caatcccggc aacccttacc
     6961 tgggcaacgc gacgcaggac ggcttctgca ccttcggccc gccggccgcc aagtccctgc
     7021 tctgcgaggt cgcctcccgt tgtctcaagg ccacctggca caagaagtgg caggtccacc
     7081 gccgtctgcg gcccgaggcc tacgccggcc gcctccacgt ccacctgaac gaatcgccgg
     7141 gccgctacga aggcgccttc caccccgacc ttttcggcgc ccgcgccctc gacaggatcg
     7201 aatccttcaa cggcggcggc cacctgctgc cgcaggcctt ccccgaggga tcgccgacgc
     7261 acccctccta cacggccggc cacgccacgg tcgccggcgc ctgcgtcacc atcctcaagg
     7321 cgctcttcga caccgtgggc cggctgatcg cgagcccggt cgtcccgaac gccgacggct
     7381 cggccctggt cccgtacacc ggcccgaccc tcaccgtcga gggcgagctc aacaagctgg
     7441 cctccaacat cgcctccggc cgcaacgtcg cgggcgtcca ctggcgcacg gacagccatg
     7501 cctcgctccg catcggtcag gaggtcgcca tcagtctcct cgaggagcag acgctgatcc
     7561 acaacgagat gtccccgtcc ttccacttcc ggggcttcga cggcgagacg atcgtggtgc
     7621 gggatggcgg agtgttcgac agctaggggc agccgccccg gggcttgcca acgaattgca
     7681 ctagtcatat aactagtctg cttgaggcct ggaggtgccc atgagtgcag atcgatccgc
     7741 aaagcgatgg ccggtccagg atgccaaggc tcgcttcagt gagttcctgg aggcgagcct
     7801 gaacgaggga ccgcagatcg tcaccaggcg gggtgtggag accgctgttc tggtcccttt
     7861 cgaggagtgg cgccgcctcc agagctcggc acggccgaca ctcaaggagt tgctactcgc
     7921 cgaggagtcg cgtgccgaga tcccggtgcc ctcgcgtcat cggtggcgcc ggcgtacgcc
     7981 cgaagcgctc gagtaggtgc acatgtacct ccttgacacc aacgtgatct cggagctgag
     8041 gcgcccccgg cctcacgggg ctgtggtcgc ctggcttgaa ggtgtggcgg accacgacct
     8101 acatctgtcc gccgttaccc tgggcgagct tcaggccggc gtcgagatca cccgcgacca
     8161 ggatccgggg aaggcttccc agatcgagga ctggatcgac caggttgccc agacctggaa
     8221 tgtcctgcct ctggatgggc gtaccttccg tgtctgggcg aagctgatgc accggcgctc
     8281 tgacgacctg ctggcagacg cgctcatcgc cgctacggcc atggtccatc atctagtcgt
     8341 cgtcactcgg aacgtccgcg acttcgcgcc gctgggggtc gagactctcg acccgttcaa
     8401 ggcggggtga ccggtccggc atccgaaacg ctcttcaaaa tgccgcttga cttacacaag
     8461 gcgtcctcat agaattccat caaacctcac ggcgcgatcc tcatcgcgtc ccctggaaag
     8521 gaggatctcc ggacctggaa ggacttgaac ccgcggtacc cgctggagac gtcgtagccg
     8581 tgcagtcgct gtacgcagct cgaagcgggg ccgggtcgtc cggcccctga cctgctgagc
     8641 ggcccgggag cgaggcggaa tctcgccccc gggcctactt cacaagggag ttttcatccc
     8701 atgcaaagcg atgacgatcg taacaccccc cgtctgccca acatcttcct ccgggagttc
     8761 ctggacctcc tcggccgccg caacgagccg cccgcggcgg ccgaggccgc cgtcgccggt
     8821 ccctggcaca tcgaggaggt ccacgccacc ttctacgccg tctaccggtc cggagagtcc
     8881 gtcgcccggg gtcaccgtcc cctggcgatc ctcaccgacc ccaaccacgc cctcctgatc
     8941 gccgccctcc tccccgccac cggccgggac ccgctctacc gtctccacaa ggaccgcacc
     9001 ccctccggct acgccataga ggccgccgga ggagaggtca tcggcccctg gccgtcttcg
     9061 accacaagct cgtagatgcc ctgcatgtcg tcgagtgcct gctccggtcg ccggcggcgc
     9121 tggcgaacct gttcgaggcg gcgggctcag tggggctgga gcggtcgggg gcggtgttgg
     9181 tggggcgggt gaatcgggag taggtaagtt gtaggatttg gcggccggcg agagccggcc
     9241 gccgtacgca aaccacagtc gtcttctctt cgagatggca cctcacgggc acgcctccag
     9301 gcgggttgag atcccgttat ccccgcgagg ggatgctaat aggtatcagt acccacttga
     9361 tcctggggac tgatcgctgg gattggctga gctttctccg cgatcgttct gtagcttttt
     9421 gatcagcagc agtgcctcgc tgaagccgag ctgacgatag gcctcaaaac gcctccatcc
     9481 ataccattgc ttgaacgtag ttgtatgagg gaagctgctg ctgtttgccg cgaacgcccg
     9541 aacataagat gggatctcgt ctccgccggg aagaatagct gccttaacat aatagacgtt
     9601 catcctctgc gtctgagggc aagagcagga gatcgagcct ttcagaacat agtcgggagg
     9661 atcctgattc ataaagtcgt caggaaatgc cttctcctca tctgcaacta cgacgggatc
     9721 gaatttccat tcatatccgg cgcacgtcat ggagcaaccc tccaggctga ccttctcctg
     9781 agagctcctc aggctgccat aggtgtagtt gctgtcggcg ctggcatcga agaccagaat
     9841 ggtgggagcg cgccgcttga ccaaagcgac gatcccaaga ttgtcgtagt gaccaccgtc
     9901 ggagatcgat atgctgttgg atcttacggt aggatagaag cgatcgatga tgacttcccg
     9961 gattacccgg tcgtatgccc acagccatcc ctgtgaggct gattgaccga agttccgcgt
    10021 atcataatgg gcgttcaggt tgaaaaacct gaggatcgcc ccgatagtat acctcttgcc
    10081 gcctcctacg aagtagggcg agattgcggc ccccgaagca gtcaccgcat tcgacaaagg
    10141 caggtccgct gtcggcctca agaatggcag aggcctttct ttgatcttta ctcgtgctgg
    10201 gaactttctg gacgggaaga agtggtcctc atcaaagtcc acgaccttga ccggcttgtc
    10261 gaaggctaca gttggtaggt agccgatgcc gggtgcgccg caatagtcgc gagtgaattc
    10321 gaacggagag ctgtcgtagt gaggccatcc tgaggcacgg gccggcagtc gatgcctctc
    10381 tttgctgccg tgatttgcaa gcgcggcatt gacaatcaga taaggagctc gggagccaag
    10441 cgggttgata ttgtgcagga gcctttttcc actcccaggc accgtctcct gatccaggag
    10501 gtaggttctt tcaattcggc ttctgtatgg atagaaagta tggtggcgat tgaacttccc
    10561 gctgacaggg cgaaagtgta ggccgacgtc aaagagcagg ttcggaacga ggcttgagac
    10621 ccagcggaat gctaggcggg gccagccgat cccggagttc ttgagcagaa aggaggtatt
    10681 tccccggagg tgcgcgacag gatccgctcc ttcgagtccc attggcccat ccccggcggg
    10741 gtccagcaga agcttgcgcg agttggagga ggcgaccaga agaccctcct gatcaagatg
    10801 attaacctct ttcccgttcc tcggcggcac aagactgctg acgtaccatg ctcctatgta
    10861 agcgccgcct gacaccgcgc tcagatagtc catctccctg agaaggttct cgtgctccaa
    10921 ggcttgaagc actcccaggt tgaacgacgc gctgcggatc ccgccaccgg agaaggccaa
    10981 tgcgatctgg gagacagagc cgggaacgtt agccactttg cgtcgttctt tgatgtactc
    11041 gcgctccaga cgatagtagt catcctgcct gccaggatct ctgcgatcct gaatggagta
    11101 gacattctga gcttgaggat ccagagtcgt gcaaccggaa agggacagcg ccagcaagag
    11161 aagcagaagg gctttcgtga gtgccgggcg tgaggctggc ataacgctct ctccttgaat
    11221 gaagaggtca gtctgcaggg gtgacagcct gccaggccat tcccgtacta ggccatctct
    11281 tctcttgcgc ccggtgcggc ggcgactctg gctgctctca tccgattcgc tttattcaaa
    11341 gcacatatag gactctgggg gttcggacgc cgtgatcgcg gatcgcaggt tcctggttgc
    11401 tctaagtagt tactgaatct agtccggctt gcaggcaatg tcaaccggct ctcgaagagc
    11461 gaacgaggaa gaacgcaacg gggctctggt gcgagggcct cgatgggaag aatggcgacg
    11521 ccggggggaa gactgtgtcc agccaagcga ggcggaggga tcgggcctcc ggcgggatga
    11581 ggtgcaggag gatgaggtgc caggcgcctg ttctcctctt tctctcctct cctgcctgtc
    11641 gtggcgggcc accggcggag cgttccgcga tgacttccct tggagcctgt caccggcgtc
    11701 gatgggcaat cggcgccatg gtgcggtact cttgagggga ggcgcctccg atgacgcgag
    11761 ccatcatctc tcctgccgac ctttcccaga ggacccaaga gatcctggag cgggtgcgcg
    11821 agggagagct tgcgatcgtc tccagcgagg gtgaagagcg gattgtcctc ctggacgccc
    11881 tcgactaccg gcttctgcga gccctggccc tctccgcgac cggcacgggc gccgatggcc
    11941 ccggagagct tctcgatgcg cgctctctcc gtgactacct cgatgagaag atcaacctcg
    12001 ggaaggcggc cgagttgctc catctttctc gcttcgagct cgaggagagc ttccagcgtc
    12061 ttgggcttcc ccttcagcgc gggcctcgct ccattgagga cgcacaagcc gagatctccg
    12121 ccgcacgccg gcatgcctga atccttcgtc cgctccaatg ccggccctcg ctgacacgac
    12181 ggtcctgaac aactttgccc agattcggag gtccgacctc ctgcgatcgg cctatccaga
    12241 cctcgctgct ccgatcccgg tgtggaaaga gctggaggtg ggcgtccgcc ggaggttggt
    12301 tcccgcctgc gactggtcct ggttgagcat cctcgtcctg acggagcggg agcaggctcg
    12361 cgccgaggaa atcggtcgcc agttgggtgc cggcgaagcc gcttgcatcg cggtcgccgc
    12421 ctctcgggga tggctcctgc tttcagatga ctggggagcc agagagctcg cccgttctct
    12481 gggcctcgag gtctcggggt ccctcggagt cctcgaccgc ctgatcgcga agcaagtcct
    12541 gactctcgaa gaggcggatg ctcatctggc agggatggtg cggcgcggtt tccggagccc
    12601 attcagatcg ctacgcgaga tgtctcgctg agggtgacac ccgacatcga cgagcgggtg
    12661 gtgaaggacg gcggggattg atctgaggat cgaggcacac gtgtaaccaa cccggcgagg
    12721 cggaggggac ctccgcctcc cggctgtcct cctgtcggac ggaggaatcc tccggggcgc
    12781 ggccttggac ccggcggcgc ctcatcacca ttcagttgtc aatgaaccct ggactcctca
    12841 tgggccgccg gcccttcggg gccaacgaca ggccctgagg ggcgatccac tcgccctgtg
    12901 gggccggcct tcggccccaa gtccaatccc gccggacccg cggggccggc cggctgcccc
    12961 gaagggcatc ccgctcgccc cttggggcca acctttcgcc cgatggggca gcgggcttgc
    13021 cccgcgggtc ctccaactag cccggggggc cgatgatggg ccctccaggg cctcctgccc
    13081 gcccctcggg gcctactacg actgcagcga gcaggcccac aacgactcga ccgactgaaa
    13141 acgatattcc cgccagatcc gtgggttgac acacggctcc ggtgaatcta gagtgtgcca
    13201 cacagaccga ctacggtcca cgaggccgcc gcggtcctgc tctttgacaa tcgatgtaca
    13261 cggttcgagc gcggcgcctc ggcggagcgc cgcgttcgga cgtttttttc aggaggcctc
    13321 cgccggccgg cgcttcctct tgtggcctcc ttcgttagga tgtaggcaca aggtggaggc
    13381 gagatgagcc agcacacggt gggtattcgg caattgaagg agcagctcag tcactatgtg
    13441 gagcaggtga agactggaga cacggtgctg atcaccgaga agggaaagcc cgttggccgg
    13501 attgtccctg tagcgccgtc tatccgggag cgtctggagc aactcgcgga agcgaacctc
    13561 cctgcctgga gcggcaagaa actcggtcct caggcgcctg tggtgcctag cgggggggca
    13621 aagactgtcg cagatctgct cctcgaagac cgggaatgat tctctatctg gatgcgagcg
    13681 cttggtcaag cgcgatgtct acgagcgagg cactgacgag atcctacggg tgatcagcga
    13741 aacggctgcc cgcttgcccg aatgacctgc caaacctcct cgattcctgg aagccggtcg
    13801 ggtagcgccg gacgtttcct tccggaggcc tccgccggcc ggcgcgccga gccttcattt
    13861 aagcacttac ataagtaggg gatgacttga ggccgcggga gccatcctcc tctcatccgg
    13921 ccacatccaa cggtgtaggt gcttgcggct cgatgacctt tggataagga ggcgtacgtg
    13981 agactcagaa cgatgtcctt cctgctcatg ctgtcactcc tgctcggctt ctccccggtg
    14041 tccgcagacc ccgtcatcta ccgcggcatc gacgtcttcg ccaccgcggc gaacggcaag
    14101 acgttctatg acttcgccca acgcccgatc cctgccggct tcttctgcaa gggctccaag
    14161 cctttcgccg gtcgggtggc attcaagggc ctgccgctcg cgactgaggt tccggggcag
    14221 ctctggggtg ccgacacggt ggtggagcgg ctcgacgatg ccgtcttcga cgagaagggg
    14281 gtcgcaacga cccgcctcca gtttcgggct ctgtcgctgg tcagcatcgc cccgatcaaa
    14341 accggttgcg gcgccttcga cgtctatctc tccctcggcg gcaagcagcg ggtgacgacc
    14401 atgagcatcc accgcaccca ggagcgcggc ggggagttcg tcggccctct tgccgtcgat
    14461 gtgcggatga ggttcgtccc ggtgaagccg gcgcgtggca agggcagccc gcggaagctc
    14521 gagctacccg ggagcatcac cttccccggc gcgccgatgc cgtggagcct ctcgtccagc
    14581 ccgctggcga agcggatcga cctcgtggtc gtcgacaccg atggggatca ggctccggat
    14641 agcctgcttc ccgggacctc gaacttcgcg ccgggtcagt cgccggacag cacctggtcg
    14701 aacaagggcc tctgcagctg ctgtccggga gagcagtgcc acgctgatga cggcgagatg
    14761 cattgcttca cggtgaccat gtgcccgaat acgcattctt gttgctgaag gcgggcagcg
    14821 atcccccctc tccgggcagg agagggggaa cgtgacagct acttgcggtc ggcctggtag
    14881 atgggacgga tgtccaccgg gacgtccgag agcttggcga tggcgttgcg gaggttgtcg
    14941 ccggctttgc cgtaggtgtc gaggaacttc ttggtccccg cgtagtcgcc gcgggcctgg
    15001 agcatcagca tctcggcgag cagctcgcgc agagcgccgg ggaacttcgc cgagacggcg
    15061 cggaagcggc cctcctggtc cacctccagg gcgcccttgg cgatcaggta gttgaactgc
    15121 gccaccacgc cctggccgtg cgcctcgtcg acgccgaagc gggccgaacg gaagaggccg
    15181 gcgacgtagg tgggctcgag cgacttccgg agctcggccg gcatctcctt gcggtcgatc
    15241 agggcgagga tgttgtacat ccccatcacg tccgccttgg cctcctcgag cgtcgcgtac
    15301 agctccttga gctccagccg cgcctcggtc ttgcggccgt tcacggtgat gctgcccggt
    15361 cccaggccgt gcgacagctc gtggtgcagc acctcgtcga agtaggcctc gaaggtgagg
    15421 tcggcgacct ggtccttggc gatcacgcgc tcggcgatcg gcaccaggat cttgtcgtac
    15481 ttggcgcgca tggtgttgcg cagcagaacc ttcttcgagc ccttcgcctc ccgtacccgc
    15541 tcgtcgttcg gcaggttgaa ggcgatggtc tgcacgccgg cccgcgtgtc gccggcggtg
    15601 aagacggtgt ccacgacgcg gatcgggctc tcggtgccgc ggttcttgtt cttgtgctcg
    15661 tcggggatcg gcaggttgcc ctccagccag gggaggcgct ccttgtagcg cgccagcgcc
    15721 gccgactcct tcggcaggtt caccgtcacg aaggattcga acgccgcctt gtagccgaac
    15781 aggccgtcct cgtaggtctc gtaggggccg atggtcacct cgaccggcgc gtccaggtcc
    15841 atccacgcga cgtcactctc atagtagtcg tcggagagga aggcgtcggc gcgggtggtc
    15901 aggtagcgct tgagcgaggc gttgtcggtg atcccggccg cctggcgcag cagcttcgcg
    15961 gccggctcga gccaggcctt gtactcctgc gagtagggca cggccgtcaa ttccttgtca
    16021 gggccgcggc ggatgacggt gaagttcgag gtgaagcgct ccttgtcgcc gggattcttg
    16081 gcgatccagg cctcgaactc ttccttggtc atgtcctcgg ggtagtagcc ggcgccctcg
    16141 gggtgcttga ggtcgccgat gaacggctcc gactccgcca gccggtccca cggcccgaag
    16201 ttgatgcgga agtactcgcg ggcggcttcg gcgttctccc cctgccagcc ggcgatctcg
    16261 cgctgcatct cggggttccc cgcccaggcc tggcgcagga agatgtcgtc catgtagtgc
    16321 gaggcctgga tcagcaggtc gagcacctgg cggtcggagg ccgacagggc ggagaggtcc
    16381 gcctggagcg ggatgggggc gaacttcgcc acccgggatg cgatatcagg cacgatcttt
    16441 ccctccgccc ctgcgggagc cgtggagccg gagcccgccg aggcggcctc gccattggcg
    16501 ggcgcgcccg ccgacgcatc ctccccgccg gtgcacgcca gcgtggcgag gctcccgaga
    16561 cagaggatgc agaggatcat cagacgcatc ggttctcctt tcgttcacgt ccatcgcgcg
    16621 tatcgtggca caccgatcgc atggctccaa accggaggct ccgatggacg gagcccggct
    16681 tcgaggtggg gaagcacatg gccgaccgag tcaaaccgta tcgtgccagc gtcaagtact
    16741 ggctgctgag cacggacccc gaggattaca gctacgacga tctggacgcc gacggcagca
    16801 ccgtctggga cggcatcacc gattacgtcg acctcaagaa cctgcgcgac atggacaagg
    16861 gcgacctggc gctgatcttc gagaccggcg ccgagccggc cgtcgtgggc ctcgccacca
    16921 tcgcctcgga cccgtacccc gatcccgagc agggggacgc caccgtctat gtggtggacg
    16981 tggagcccga gcagtggttc gaggagccgg tgcgcctggc cgacttccag gacgatccgg
    17041 cgttccagga gctcgatttc ctcaacgcgc cggagcgcgg agtggtctcc gtccccgagc
    17101 cgctgtggaa ccggatcctc gagatgggcc gcgtgccggc ggaggagcgg gtgtgagacg
    17161 ggcggccacg gcggtggtcc tggcagtcct ggcggccggt gccgccgtcg ggccggccgc
    17221 cgcccaggac atcgccttca accgctgggg cgtccgcgcc ggcgcttcgg acgatcccga
    17281 ccaggtcgtg gtcggggccc aggccgactt cggcacgttc gtgcgcaacg tgcgcttcca
    17341 gccgcacgcc gacctggggc tgggcgacga cgtgacgatc ttctcgctga cggcgccggc
    17401 gcactacctg ctgccggtcg aggggggctt caccctctac ggcggcggcg ggctcaccct
    17461 cgcctggatc gacagcgacc gccccgagga ccgggacgac tcggatttcg agatcacccc
    17521 catggtggcc ggcggcgccg agtggccggc cggtcccggc ctgctctccg tggagctgaa
    17581 cctgagcggc ggcgacctgc ccggggtgaa gctggtggcg ggctggatgt tctagcggct
    17641 ctaatggtcc ggcgcgtcct gccgggcggt caggatggcg agctccaggt actcctctcc
    17701 ttccagcagg taggtgcggt gcggctccac cgcgaaggtg acgaccacct ccagggtctc
    17761 ctccccgtct tcctgccgga tcagctccgc cagccgggga tcgagctggt cgagctggta
    17821 gatccaggac tcgacggcaa tgccgcgggc ctcgacggag ggacgggtgc cgggcctgcc
    17881 cgggtcccag gcggggacct gcttctggct gtagcgcagc accggcggcg agcccagcag
    17941 gatgaaggcg cgccccttgt cggtcatgct cccccgcgtc ccgtcctctg aatagagctt
    18001 gtcggcggcc tcgacccgct cgtagaaggt acgggcgtac tcgttggcgg gggtcgccgg
    18061 gtcggggtcc cggcggtccc agaacgtctc ggtgaatagc gccgcgtcgc gggtcgaggt
    18121 cagttgccgg gcctgccgct gctcctcggg gagcatgagc cagcgcgtgg ggccgtccag
    18181 caggccgccg atccccggcg cctgcgccgg gccggaggac tggcagctca gggcgagccc
    18241 cgggaggagc agggacagga gcgccgccgg gagccgtcct gccatgctgg agcgctgggc
    18301 catgggcgcc attcgccgtc agtgtacgcc ctggtcccca cccggctccg gcgcctcgtg
    18361 gatcgggccg aaggtctgct cgtagcgctc caggttcatg ctgagcgccc ggatgatcct
    18421 cttcatgtgg ccggggctgg tgatgatgcg cgcggtgacc accccctggg gaggcatcag
    18481 gttgatgaag tcgaggatga actcctcgcg ggtatgcgcg atgcgcagca ggttcgagta
    18541 acggcccttc agctcgtcgt catcgatctt gacgttgagt ccctcgccgg gctcggccat
    18601 gcatgacctc cttggagagt gcccgttgaa tgttccggat gccctgatgc tatcccatca
    18661 cggggcatcc ggcatccgca ggtagaccgc gaggtcggcg acgttggtgc cggtggggcc
    18721 ggtctcgagc agcgacccgg gcacggcgga cagcgcgccg taggagtcgt ggcgctccag
    18781 ggcggcccgc ggatccttcc ccgcccgccg caccgcctcc caggtgccgc cgtccaccac
    18841 cgcgccggcc cagcgggtgg ggccgtcctc gccgtcggtg gccagggcga ggaccacctc
    18901 ccgcgctccg gcttcgggag gcgccgccgc cagctcccgc gccgccgcga gggccagctc
    18961 accgttgcgg ccgccgcgcc cggtccctcc ggtcagcgtg acggtggtct cgccgcccag
    19021 gaccagggcc acgccgttct ccccgtgacc cagggcgcgc ccccgtgcca ccagcacgcg
    19081 ggcggtgcgc gccgcctccc cctccagcgg gccgtccacc gccgtgaagc cgttgcgccg
    19141 cgcctcggcc aggacggcgg cgacagcggt gccgttgccc cccaccacga cggtctccac
    19201 ccgcaccagc gccgggtcac ccggcttgac cgtctcgggg accacccccc tggcccccgc
    19261 ctccaggtgg cggcggaccg cttccggcag gccgactcct tcgacagcgg cgagggcttc
    19321 ggcgaaggtg gtgggatcgg cggcgaacgg gccggaggcg agggtcgacg ggtcgtcccc
    19381 ggggacgtcc gagaggagca gggcgagcac gcggccggcg gaggcggtcc gtagcgcccc
    19441 tccccccttg aaggaggaga ggtgcttgcg cacggcgttg accgcctgga tgggtagccc
    19501 ggaggcgagg agggcgcgct gggtggcggc gaggtcctcc agggtgatac cgggggccgg
    19561 cacctcgacg caggccgagg cgccgcccga cagcagggcg agcagggcct ggtccggagc
    19621 gagctggccg agccagcgga ccgcgcggtc gccggccgcg aggctctggc ggtcggggag
    19681 ggggtggccg ccggcgagct gctcccagcc gggatcgagg agggcggggc tggagtgggg
    19741 gcggatcagc aggcggcgcg ccgggctgga gaggacgccg cgggtgccgc gcgccaggcc
    19801 ggcggcggcc ttgccgaggg ccaggatggc gccgaaccgc gcgccggaca tctgtccgag
    19861 gcgccgggtc agctcttccc gcaccagctt ttcgggatcg gcggccgcca ccgccacggc
    19921 cgccagcctg cgcgcgaagt cctggatctc caagtcctct ccctccctct actcctcctc
    19981 ccagcggact ccgcccgaga actcccagcg gtgctccggg aagtagaccc cgacgccgcg
    20041 ctgtggcgag ctcactgtga aggcgagcgc cttccgttga tagtcgtacg gcgcgccgtc
    20101 gcgcgcgtgg acggccacgt cgaccagata gtcgccgggg gcgaagcgca gcgccgggca
    20161 ggtcagccgc accgtcgtct cgccggccag ctcgcccggc acgaagccgg cgaggtcggt
    20221 gttggtcccc cagcactcga agccgcgcgg ggtggagacg gcgacgccga agacgaagtc
    20281 gcagagcggc cggtcggccc gggcgcggat ctcgaacacc gcctcctcgc cggtctccag
    20341 gtggtagcgc tcggtgccgc ccacgagcag ccggacgccg gtgatctcgg cgtcccgcga
    20401 cccccagcgc agcggctcgg cctccccctg ggccgcggcg ccggacccgc tggcccctcc
    20461 agccggtggc tccgcggcgg gcggctgctc ggcggcggct tcgtcgcgct gctgcttggc
    20521 ggcgcggttc gcctccccct cgccctcggc gaccgcctga cggtaggcgt cgatgacccg
    20581 gcgcggcgtc cccaccaggc gctgcacgcc gttgtcgagc cagagcaccc ggtcgcagac
    20641 gttttccacc aggtccagcg agtggctcac caggagcagg gtcttgccgg cggcgaggaa
    20701 ctcctcgatg cgccggatgc agcggtgggc gaaggcctcg tcgccgaccg ccagcacctc
    20761 gtcgaccagc agcacctcgg ggtcggtgtg gacggcgacc gcgaacccga ggcggacgta
    20821 catgcccgag gagtagttct tgaccggctc ctccatgaag tcgccgaggc cggagaactc
    20881 gacgatctcg tcgtagcggc tttcgatcag cttcctcgac aggccgagga cggcgccgtt
    20941 gatgaagacg ttctcgcgcc cggagatctc ggggtgaaag ccggcgccga gctcgatcag
    21001 cgccgccacc cggccgcgca cggtgatccg cccggtggtg ggcttgagca tgccggcgac
    21061 cagcttgagc agcgtcgact tgccggagcc gttgccgccg atgacgccga acgcctcgcc
    21121 cggagcgacg gtgaagtcga ccccttcgag ggcggcgatg gcctcctcgc gcttgaggtc
    21181 gcgggtgagg ctgccgccga ccagggcgct cttgagcgtc cgcagccggt cgccgggcat
    21241 ggtgcggcga tagagcttgc tcagcccctc gacacggatc gcctcgtcgg ccccgagggg
    21301 ggctgccggg gttgccggag aaagggaagc ttcgggctcg ctcatgccgc ctccaccagc
    21361 gtctcccgca ggcgctcgaa gagccccgcg cccaccaccc aggcgagcag gctgaccagc
    21421 gccatctgga tccagaggtc gggctccggc gcccgtcccc ggaagaggac ctcctggtag
    21481 gccagggtga acggggtgaa cgggttgagc cgcacgatcc accagatgag gggaaaggcg
    21541 cgcaccgagt ccagcgggta gaggatcggg gtgaggaaga acatcagggt gagcaggttc
    21601 tggaggatgt cccgcacgtc cttgaagtgg acggtgagcg ccgcggcgcc gagggcgatc
    21661 cccgccacca tcggcaggtg gaggaggagg atggccggca gcagcaccgc gccggagccg
    21721 cccaccgggt actgcagcag ccagccggcg aacagggcga ccgccacgat cggcagggcg
    21781 aatagcaggt gcacgaagtt ggagagcacg gtcaccatcg gcagcagctc ggccgggaag
    21841 accgccttgc ggatcagccc cgagttggcg atcagcgaca tggagccttc gagggtcgag
    21901 gtggagagcc agatccaggg gaagaggccg cagaccacga acagcgcgta cggctcgatc
    21961 cccggcaccc gcgactggaa aacgacgctg aagacgaagg tgtagaccgc cagcagcagc
    22021 agcgggttgg ccagcgacca gaggaacccc agcaccgacc cccggtagcg cgccttgagc
    22081 tcgcgggagg tcagcgtcgc cagcaggcca cggaagcgga cgatctggct ggcgacccgg
    22141 aacatgatgg tcttttcgag ggatgccttg gacgggacgt ggctgttcta acccaccccc
    22201 gggtgggtgt caaggcgacc gggggtggga gagggctgct agactccgcc cgcaatgccc
    22261 gagtggagcc cggtggacct gctgcccggc ctctgggccg gcgctctgct ccttgtcctc
    22321 gccgccgcgc tgcggcgctg gtatgacccg gtgccggcgc cggtcctcgc cgccttcggc
    22381 gccgccgtgg ccctgctgtt cggcggcgtc ctgttcggcg gcggcatcct cctccccctg
    22441 gacaacctgc gggggcatgt gccgttccag gggctggcgc cggccgagcc gcacggcaat
    22501 atcctccagg gggacctgat cgagctggtc gcgccgtccc aggcggcggt gggggaggcg
    22561 ctgggggaga ggcgctggcc gctctggaac ccgcgggtgg gcgccggcat ggcgctgatc
    22621 tccgatcccc aggcgcagcc gttccagccg ctggtgctcc tcgccgctcc catgccgctg
    22681 gagcgggcgc cgggggtgac cgccgcgctg cgggtcctcg tggccctgac cttctccttc
    22741 ttgtggatgc gccgccaggg gctgggggcg gtccccgccc tcgccggcgc cttcgcctat
    22801 ggcctcggcg gcttcctcct gctctgggtg ggatggcccc tcgccaactc ggcggctctc
    22861 ctccccctgg ttctctacgc tctcaccctg tgcggccagc gggggctgcg ccgcgacgcc
    22921 gccctcctcg ccggcggtct cctcgccctg ctgctggccg gccatcccga gaccatcctc
    22981 ttcggactgg cgctcgccgc cgtcttcctc ctggaccagg tccgccggcg cccgccgggg
    23041 acgcgccggc ctctcctccg taccgccgcc ctggccgccg tggccgcggc gctggccgcg
    23101 gcgccggtgc tgctccccgt ggcccacgat gtcccccgtt ccatgcgggc ggcccgggtg
    23161 agcgatccgg ccgtccattc atcgcccgcc ggcggccttg ccccccgctg gctgccgatc
    23221 gcggcgccca acgcctacgg caacagccgc ttcatccact actgggggct ctccaacacc
    23281 aacgaggacg ccgccggctt catcgggacc gccgctctcc tcgccgcgct cctgtccctg
    23341 ggagcccggc ggcgcttccc gcaggagggc acggccctgg ggatcgcggg cctctgcctc
    23401 gtggggctcg ccctgcctgc cttcgtcccc ctgccggact ccctggcgag ccggcgggtt
    23461 ctcctgccgt tcgtgctctg cctcgcctac ctcgccgcct gcgcgctgga gcgcttccgt
    23521 cagggcgagg tccggcggcg cgcggtgctg gccgcggcgg cggtgctggc cggggtcgtc
    23581 gtctgggggt acgtgaccca tccggacccc tccgatccgg agcggctggc ggtcttcagg
    23641 tacggatggc tcctctggca gatgcggttc ctggtgctgg gcgcgctgct cctcgccctg
    23701 cccggccggg gagaggggaa ccgctgggtc cggacggcgg cggccggggg gctcgcgctg
    23761 ctggtggcgg tggagctggt cctgatccac cggccggcca atccgcccat gccgcgccgg
    23821 ctcgccctgc cggtgacccc gccgctcgag ctcctgcgcg agagcctcgc cgaggaccgc
    23881 gccgggctga ccggcctgcg aatggcggcg ctgggccggg cgctcccccc caacctggcc
    23941 tccctgtacg gtctccccga cgcccgggtc tacaacccca tggcgcccca ggcctatgtc
    24001 gaggtgacgg cgccgatcac cgccgcctgg tggggggagc tgccggaatt cggcgcgccg
    24061 gaccacccgc tctatccgcg gctcggcgtc cgctatctga tgacggagcc cggcgagcgc
    24121 ctgcccgcgc cgtggcgtct caccctggag gatgccggcg cctggatatg ggagcacccg
    24181 cgggcgctcc cccggctctt cctggagggg cggggccggg gcgccctgcg gatcacgcgg
    24241 ttgaagagcg cctggatcac cgcgcgggtc cggcccagag aaggtggcct cctcgactcc
    24301 agcctctatc aggacggggg atggcggctg ctggtggacc ggcggctccg cccggggcac
    24361 ccgaacggcg ggccgttcgt cgttgccgag ctgcccgaag gggcgcggcg ggtcgacctc
    24421 gtctaccggc cgcgggcatt cctcctcgga tgcctgctgg cccccctggg tctcgccctc
    24481 gcggcggcgc tttcggtgcc gccgcccggt agaaggtaga atggcgccat gagcgagacc
    24541 cggaagctga cgatccgctg ccctgattgc gggtccgacc tggtggtgga cgccgccacc
    24601 ggacaggtgc tgtcccaccg caagcccaag caacccttgg ccggaggcaa ggacttcgac
    24661 gccctgttgc gcggcttgga cgaggacaag gccaaggccg aggacatctt ccagcgcgag
    24721 ttcgaggcga tgaaggaccg cgaccgcatc atggaagaga agttccgcga ggcgatgaag
    24781 cgggcggagg aagacccgga cgaggggcct ccgcgccggc ctttcgacct ggattgaggg
    24841 ccccgatgag gacaatcggg ctccgatccc ggccgtggta aaatcttccc cggtttttcg
    24901 gcccgccccc gccgccgtgg tccgagccca acctcgagat ccgccgccgc ccgtgagcat
    24961 cgccgggcgc ggcgtcgatc gaacgaagac tgagcatccg acgagcccgg ccccgggctc
    25021 gggaaaggga atcgaacctt gagcccatgg tggttgcgcc cgacagcaga gggcgccgcg
    25081 aaagacgaca accgagagca ggacaagtcc ccctccacgc cccctccccc cgccgaggct
    25141 ccgcagccgg ccgccccgga gcccgcagag gctcccgtcg aggcacgggc tgacgaggcc
    25201 cggacggccg aggctcggca gcccgagacc tggccggcgg atccgcgcgt cgaggagccc
    25261 cataccgggg tcaccggaca cggcgggacc agcgaggccg gcgagccgcc gctgccgtgg
    25321 gagactcccc gcgagtcccc cgccgagctg gccggtgagc ccgatacccc cgagcttctc
    25381 cccggcgtac ccgaggccgt cgagctctcc ctggaagccg tcgagccgct ccccgtcggc
    25441 tacaccgccg aggacggcga cgacgattcc gaggacgacg agatccccgg cgcggtggcc
    25501 gagaccgccg aaggggaggg cgccgaacgg tcggaggagg gcccggcccg caagaagcgc
    25561 tcccgccgca gccgcggcgg ccgccgccgc aacggccgcg acgccgccac ggacagcgcc
    25621 gaagccgcgg acgacgagga cgaggctctc gccgaggagc cgacccccat caccgtgccg
    25681 ccgcctcctc ccccctccat tcccgcccgc accgcccggg aggggcgtga acgggaggga
    25741 cgcgaagggc gcgaaggccg ggagggacgg gagcgcgacc ggggggaccg cgagccgcgt
    25801 gagccccgcg agtcccgcga gagccgcgag agctactcct tcgacgccca gccgccgttc
    25861 cagcccgtcc gcgggcgcga cagctacgtc ttcgccccga gcgatatgga gggcggcttc
    25921 ggcggcggcc ccttcggcgg ccgcgagatc gtcatctccg tgccggagcg acagcgcgcc
    25981 ggcagcgacg agcgcaagat cgccctcttc tgcgacctcg agaacatcgc cctcggcgtg
    26041 cgcgactcgg agatcagcaa gttcgacatc gacctggtcc tcgagcgcct cctcgaaaag
    26101 ggcaagatca tcgtcaagaa ggcctatgcc gactgggagc gctacagcga gtacaagcgc
    26161 cccttccacg aggccgccat cgagctgatc gacatcccgc agaagtacta ctcgggcaag
    26221 aacagcgccg acatcaagat ggtggtcgac gccatggacc tctcctactc caaggagcac
    26281 ctcgacacct tcgtcatgct gtccggcgac agcgacttct cgccgctggt gtcgaagctc
    26341 aaggagaaca acaagtacgt catcggcgtc ggcgtcaaga actcgtcgtc caacctgctg
    26401 atcgacaact gcgacgagtt cctctactac gaggacgtct ggcgcgacgc ccagaagggg
    26461 cccaagctcg acggcctcaa caagaagtcg gccgaggtgt tcagcctgat ggtcgacgcc
    26521 atccaggcgc tcctgcgcga gaacaaggac gtgctctggg gctcgatgat caagcagacc
    26581 atgcagcgca agaagccctc cttctcggag agctactacg gctacaccac cttctccgag
    26641 ctgctcgagg acgccgagcg caagcagatc atcaagctta agaaggatca gcgctccggg
    26701 acctacatcg tcaccggctt cgccaagcgg taggaccgcc ccactactct ttccggatcg
    26761 tccccaggag cctgagcagc tcctggggat cgatcggctt caccaggtgg acgtcgaagc
    26821 ccgcctcccg ggactgcgcc ttgtccgtct cctgcccata gccggaggcg gcgatgagca
    26881 cgacctcccg catcgccgga ttctggcgca gccgccgggc cacctcgtag ccgtccatca
    26941 ccggcaggcc gatgtcgagc aggatggcgt ccggccggtc ggcggtcgcc atctccacgg
    27001 ccgccaggcc gtcgaaggcc agccgcacct catgccccgc gagatgcagc agcatgccca
    27061 tggtctcggc ggtatcctcg ctgtcgtcca ccaccaggac ccgcagggct gacgtcttgg
    27121 tgggaggaga gggctgctcg ctctggccgc cgcccggctc cgcgacgggc agccggacgg
    27181 tgagctcgct gcccttctcc ggcccctcgc tgtgtgcctc gatggtgccg ccgtgcagct
    27241 ccagcagctt cctggcgagg gtcaggccga cgccgaggcc cccctgcgat cgggccagcg
    27301 tgcggcttcc ctgggtgaag gggtcgaaca tactggcgag gagctcgggg gggatgccga
    27361 cgcccgtgtc acggacccgc aggacgacct ggtccctctg ccgttctgcg ccgatgtgga
    27421 tgcggccccc cggctcggtg tacttcgccg cgttgttcag caggttggcg acgacctgct
    27481 ccagccgggt ggcgtcgccc tcgagccaca ccgtctcggc ggggagcgac tgggtgagct
    27541 cgtgccgccg cgcatcgatc agggggtagg cggtctggac ggcgttctcc acgatgcggt
    27601 gcagctccac ccgctccttg cgcagctcga tcttgttcag ggtgatgcgg ctgacgtcga
    27661 gcaggtcgtc gatgaggcgg gcgagctggc gcacctgccg ctcgatgatg ctcttggcct
    27721 gccgctcggt gcgttcgtcc ccggggtgac ggtcgaggag gtgaagggcg ttgaagatgg
    27781 gggccatggg attgcgcagc tcgtgcgcca gcacggcgag gaactcgtcc ttggcccggt
    27841 cggcccgggc cagcgcctcg gcgcggctct gcagctcctc gtcgagccgc ttgcgctcgg
    27901 tgtagtcgcg caggatcttg gcgaagccct ggagggtccc tgcctcgtcg cagagcgcca
    27961 ccgtgacgcc gttggcgaag aaccaggagc cgtccttgcg cacctgccag cggtcgtcgc
    28021 ccgccttgcc cgtcgcggcg gccatctcca gctctctccg gggataatcg tgctcgagct
    28081 gctccggggt gaagaagcag gcggagggcc ggccgaggat ctcgtcctcg cgatagccca
    28141 ggatccgctc cgccgcgacg ttccaggtgg cgacccgccc ttccgtatcc aggaggaaga
    28201 cggcgtagtc cttcagattc tccgcaaaga gatgaaagaa gtccccctca cggagcaggt
    28261 gtctcatgtc agggggtgac gcccacggcc agcggatagt ggatcgattc cagcagccac
    28321 tcgcggttct gccggacgtc ctcgacgagc gccgacacca gcgcttcacg cctctcgaac
    28381 agccggtagc cttcgcgcgg gcgcaggtag ccgcgggcct tggggtgctt ctcgaacaac
    28441 gcctcctcgc ccatgcgctc ggcccagcgg gcgtagcggg cgcggttgtc cagcagcgct
    28501 ttgaccagga aggggatgac gatggagtag tcggcctgca ggctctcggt ggtctgct
//
LOCUS       DASPJI010000351         3167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_592528, whole genome shotgun sequence.
ACCESSION   DASPJI010000351 DASPJI010000000
VERSION     DASPJI010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592528"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VEL74_09325"
     CDS             complement(<1..836)
                     /locus_tag="VEL74_09325"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HYG62768.1"
                     /translation="MKAALALVAALASLAHAAPAPPSKPVQYTVAPIMAEKRLAGLAV
                     EIRLAGDPDGETRLVLPQEWAGSNELWRHVADVRVDGAEAVREEGPAVRVISHKPGAP
                     LTVRYRVNSAWDGEPGFEYEKARPLILPGWFLFHGEGVFAAPEGRQDAPARFSWKGFP
                     ADWKLASDLDHLTGAQAGTVDDVVESVAMGGPDLHVSRRDIDGAPLRVALRGTWRFSP
                     EAFSEMVATIIRAENELWGAPARPFFVPLAPLGGGESGYSFTGTGRGDAFSVASTPAF
                     DLA"
     gene            complement(861..1304)
                     /locus_tag="VEL74_09330"
     CDS             complement(861..1304)
                     /locus_tag="VEL74_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG62769.1"
                     /translation="MTPPGAEATLRLVLDTSAYSHFRAGHPRVLDLVAAAEIVLLPVI
                     VLGELEAGFSLGRRERENQVLLAEFLAEPFVSVLPVTPSVARQYGRLFTDLRRAGTPI
                     PINDVWIAATTLDCGGHLLTFDGDFDKLPIVDRTVLPVPDPPRRS"
     gene            complement(1285..1527)
                     /locus_tag="VEL74_09335"
     CDS             complement(1285..1527)
                     /locus_tag="VEL74_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62770.1"
                     /translation="MGKQLTIRGVPDEVGQRLENLSRARGQSLNATVLSILEAAVGVD
                     ERRQRLTRYTTWTPEDLAEFNDALAAQRTLDDPAWR"
     gene            complement(1704..2087)
                     /locus_tag="VEL74_09340"
     CDS             complement(1704..2087)
                     /locus_tag="VEL74_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG62771.1"
                     /translation="MSSVVLDASAVLALLNDEPGSEVVEEAIPGAAISAVNLSEVVAK
                     LVENGMPEEAARLALGSVQLEIHAFGAELAYQAGILRPATRKAGLSLGDRACLALGKQ
                     LSLPVWTTDRNWKDVEVGVEVRVIR"
     gene            complement(2084..2326)
                     /locus_tag="VEL74_09345"
     CDS             complement(2084..2326)
                     /locus_tag="VEL74_09345"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HYG62772.1"
                     /translation="MDRVLARLGEGGRLVIPAEYRKALGIDTGDELVLVLEDQSLRVL
                     TPREGIRRAQALVRSYIPKGRRLSEELVRERRQESE"
     gene            complement(2966..>3167)
                     /locus_tag="VEL74_09350"
     CDS             complement(2966..>3167)
                     /locus_tag="VEL74_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HYG62773.1"
                     /translation="MMLWKDLRAVMLASVVVGVGITLGGLGLSFAYDLPSGPAIILLG
                     AVLLLAAYAVRRLSRRGAFPRP"
BASE COUNT          569 a         1087 c         1024 g          487 t
ORIGIN      
        1 gcgagatcga acgcgggggt ggaggccacc gagaaggcgt caccgcggcc ggtgccggtg
       61 aaggaatacc cggactcccc ccctcccaac ggcgccaggg gaacgaagaa gggccgcgcg
      121 ggagcacccc acagctcgtt ctcggcccgg atgatcgtcg ccaccatctc cgagaacgcc
      181 tccggtgaga agcgccaggt tccccggagg gcgacgcgca gcggcgcgcc atcgatgtcg
      241 cgccgggaga cgtgcagatc gggccctccc atcgcgacgc tctcgacgac atcgtcgacc
      301 gtaccagcct gggcgccggt gagatggtcg agatcggagg cgagcttcca gtccgcgggg
      361 aagcccttcc aggagaaccg ggcgggggcg tcctgccggc cctccggagc ggcgaagacc
      421 ccctcgccgt gaaataagaa ccagccgggg aggatcagag ggcgggcctt ctcatactcg
      481 aagcccggct cgccgtccca ggccgaattc acccgatagc gcaccgtcag cggcgcgccg
      541 ggcttatggg agatgacgcg caccgccggc ccctcctcgc ggaccgcctc ggcgccgtcc
      601 acccggacat cggcgacgtg ccgccacagc tcgttgctgc cggcccactc ctgaggcagc
      661 accagccggg tctcgccatc cggatcaccc gcgagtcgga tctcgacggc cagcccggcg
      721 agccgcttct cggccatgat gggggcgacc gtgtattgga ctggtttgct gggtggagcg
      781 ggtgcggcgt gggcgagcga tgcgagagcg gcgacgagag ccaatgctgc cttcatgatc
      841 tctctactcg atgatgaggt tcaggaccgc cgcggtggat ccggaacagg caagacggtg
      901 cggtcgacga tcgggagctt gtcgaagtcc ccatcgaagg tcaggagatg gccgccgcaa
      961 tcgagggtgg tcgcggcgat ccagacatca ttgatcggga tcggagtgcc ggctctccgc
     1021 aggtcggtaa agagcctccc gtactggcga gcgaccgaag gtgtgaccgg caagaccgac
     1081 acgaagggtt cggccaggaa ctcggccagg aggacttgat tctcgcgctc tcgccggccc
     1141 aggctgaagc cggcctccag ctcgccgagg acgatgacgg gaaggagaac gatctccgcc
     1201 gcggcgacca gatcgaggac ccgagggtga ccggctcgga agtgcgaata cgccgaggtg
     1261 tccaggacga gccggagagt cgcctcagcg ccaggcgggg tcatcgaggg tccgctgagc
     1321 cgcaagagcg tcgttgaatt ccgccagatc ctccggcgtc caggtcgtat agcgggtcag
     1381 cctttgccgt cgctcgtcca ccccgaccgc cgcctcgaga atggagagta cggtggcgtt
     1441 gaggctctgg ccccgtgccc ggctcaaatt ctcgaggcgc tggcctacct catccggaac
     1501 cccgcggatc gtcagttgct tgcccatgca ggcagtgtag cacgagagtg taggcacgca
     1561 ggcaagatcc ggtcctcggc tacagtctcg accgtgccgt cagcagctcc gcggcggaga
     1621 gcgcgccgac tccccgggcg tcgcccgtca agtggaccag gaaatcccgc gtctccaggt
     1681 gatgctcccg gatcggctcc tcgttatcgg atcaccctca cctcgacacc gacctccaca
     1741 tccttccagt tcctatccgt ggtccagacc gggagagaaa gctgctttcc cagcgcgagg
     1801 caggcacgat cccccaacga gagccccgcc ttcctggttg cgggcctcag aatgccggcc
     1861 tgataagcca actcggctcc gaacgcgtgg atctccagtt ggacgctgcc cagagcaagc
     1921 cgggcagcct cctcaggcat gccgttctcg acgagcttgg ccacgacttc cgagaggttc
     1981 acggcgctga tcgccgcccc gggtatcgcc tcctcgacca cctcgctccc gggttcgtca
     2041 ttgaggagag ccaggacagc ggaggcatcg aggacgacac tactcactct gactcctgac
     2101 ggcgctctcg aaccagctcc tcggacaggc ggcgcccctt cggaatataa gaccgcacca
     2161 gggcctgggc cctccggatc ccttcgcggg gagtcagaac ccgaaggctc tgatcctcca
     2221 gcaccagtac gagctcatct cccgtgtcga tccctagcgc cttgcggtac tccgcgggaa
     2281 tgaccagcct gccaccttcg ccaagccttg ccagaactct atccatctgc cacctcacgc
     2341 gaagcttacc atgacaacct aagagtaccg agctcctccc tctagccagc gcagcggcga
     2401 cagagtaccc gcctatgtgc tggatgcgcg aaagcggagg gatcggacct ccgcctacac
     2461 gctctctttc caccggtaca cagcccctca gggagcgcgg ccttgggtac ggcggcgccc
     2521 cctcacgatt cagttgtcaa agatcacggc ccgaatgctt ccggcgccgc ccgaaccctt
     2581 cggaatccat ccggaaggct cggatcgctt ccgaagcccc tccctgcctt cccgagtctc
     2641 ctggaacctg tccggacggc tcggatcgcg tccgaacgcc ttcgctgccg tccgaacgcc
     2701 ttcgccacct tcccgagccc cgcggatcgc tgcggaaagg tctggatgcc tgcgcagcga
     2761 tgcggaacag aactctagcc actggaacca tctatgtcaa tataatcatc tcgtcagaca
     2821 tcatggtacg ctctccggac gagaggggtg gcatgggcga agcccggtcc ggctcctcca
     2881 gctctttgac agatgacgta catgtccgac gcggcgccga gggggaggcg ccgcgtgtgg
     2941 gcgtgctttg tttctcaggg cccagctatg ggcgcgggaa ggcgccgcgc cgggacagcc
     3001 ggcggacggc gtaggcggcg aggaggagga cggctcccag gaggatgatc gccgggccgg
     3061 aggggaggtc gtaggcgaag gagaggccga ggccgcccag ggtgatcccc actcccacga
     3121 cgacggaggc gagcatcacc gcgcgcaggt ccttccacag catcatc
//
LOCUS       DASPJI010000352         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_829531, whole genome shotgun sequence.
ACCESSION   DASPJI010000352 DASPJI010000000
VERSION     DASPJI010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829531"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2144)
                     /locus_tag="VEL74_09355"
     CDS             complement(<1..>2144)
                     /locus_tag="VEL74_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG62774.1"
                     /translation="HEILRTTFDVAGGEPVQRVAPSVEWSLPLVDLHALPTEARRGEA
                     KRLARRVGNHPFDLARGPMLRVVLIRLTEDEHVAGTSMHHIVGDALSTGILFREMAVA
                     YDALSTGRPVALPELPFQYADFARWQRRHLTGEVLEEQLAYWRQRLDGAPEVLELPAD
                     HPRAAVSNRGGRFASTLPASLVERLRAVAQVEGATLFMGMLAGFDALLQRWSGQDDLV
                     VGFPVGSRGRTELEGLIGIFVNLLALRVSVAGDPGLRELLSRVRQATVEAYAHQDLPF
                     EKLVAELRPDRQLSRAPVFQISFGFQNVDSPARLSSFGMSLLPSVEEPEAFDLSCGSS
                     ETPEGIQISWRYKADLFEPPTIARLAAHFETLLEGAVADPERPLSQLPLLTPAERHQV
                     VMEWSPAVNDPGTLRPPVVHEMFEEVARERADAVALQDEDLQLTYAELNRRANRLARH
                     LAAMGVGPEVAVGLCMERSPALIESMLGVLKTGGAYVPLDPAYPHERLAYLVDDAALP
                     VWVTRGEAGETLAPLARRGAAAIDVDLDGPWIAAHDGGDLGVPVDPDTLAYVMYTSGS
                     TGRPKGVEVPHRGVARLVRGDFIPFGPGEVFLPLAPPSFDASTLEVWGPLLHGGRLAL
                     PPRSGPGPLSIAALEEAVVRHGVTSLWLTTGLFHQVVEGQVEAVRQVPRLLTGGDILS
                     PAHVRRALADRPGRVLVQAYGPTENTTFTTCQ"
BASE COUNT          331 a          792 c          767 g          254 t
ORIGIN      
        1 tctgacacgt ggtgaaggtg gtgttctcgg tggggccgta ggcctggacc agcacccgcc
       61 ccggccggtc cgccaaggcg cggcggacgt gcgcaggcga caggatgtcg cccccggtca
      121 gcaggcgcgg cacctgccgc acggcctcga cctgcccctc caccacctga tggaagagcc
      181 cggtggtcag ccacaggctg gtgacgccgt ggcggacgac ggcctcctcc agcgccgcga
      241 tcgacagcgg cccgggcccc gaccggggcg gcagggccag gcgcccgcca tggagcagcg
      301 ggccccagac ctccagcgtc gaggcgtcga acgacggcgg cgcgaggggc aggaagacct
      361 cgccggggcc gaagggaatg aagtcgcccc gtaccagccg cgccaccccc cggtgcggca
      421 cctccacccc cttgggccgg cccgtcgagc ccgaggtgta catcacgtac gccagggtgt
      481 cggggtccac cggcacgccg aggtcgccac cgtcgtgcgc ggcgatccac ggcccgtcca
      541 ggtccacgtc gatggcggcg gcgccacggc gggccaatgg cgcgagcgtc tcccccgcct
      601 cgccgcgggt cacccagacc ggcagcgcgg cgtcatccac cagataggcc agccgctcgt
      661 gtggataggc ggggtccagg gggacgtagg ccccgccggt cttgagcacg ccgagcatgc
      721 tctcgatcag cgccggcgag cgctccatgc agagccccac ggccacctcg ggccccaccc
      781 ccatggccgc gaggtggcgc gccagacggt tggcgcgccg gttgagctcg gcataggtga
      841 gctggagatc ctcgtcctgc agggccacgg cgtcggcgcg ctcccgcgcc acctcctcga
      901 acatctcgtg gaccaccggg ggccggagag tccccgggtc gttgacggcc gggctccact
      961 ccatcaccac ctgatggcgc tcggccggtg tgaggagcgg caactgcgac agcgggcgct
     1021 cgggatcggc caccgccccc tcgagcaagg tctcgaaatg cgcggcgaga cgggcgatgg
     1081 tgggaggctc gaacaggtcg gccttgtagc gccaggagat ctggatcccc tccggcgtct
     1141 cgctgctgcc gcagctcagg tcgaaagcct ccggctcctc gaccgacggc agcaggctca
     1201 tgccgaagga gctgaggcgg gcgggagagt cgacgttctg gaaaccgaag gagatctgaa
     1261 agaccggcgc gcggctgagc tggcggtccg gccgcagctc cgcgaccagc ttctcgaacg
     1321 ggaggtcctg gtgagcgtag gcctccaccg tggcctggcg gacgcgggag aggagctccc
     1381 gcaggccggg gtcccccgcc acgctcaccc gcagggccag caggttcacg aagatgccga
     1441 tcagcccctc gagctcggtc cgcccgcggc tccccaccgg gaaccccacc accaggtcgt
     1501 cctgcccgga ccagcgctgg aggagcgcgt cgaagccggc gagcatgccc atgaacagcg
     1561 tcgcgccctc cacctgggca acagcgcgca gacgctccac cagggatgca ggcagcgtgg
     1621 aggcgaaccg gccgccacgg ttcgagaccg cggcgcgggg gtggtcggcc ggcagctcca
     1681 gcacctcggg cgccccatcg agccgctgcc gccagtaggc aagctgctcc tccagcacct
     1741 ctcccgtcag gtggcgccgc tgccagcggg cgaagtcggc gtactggaac ggcagctccg
     1801 gcaaggcgac cggacgcccc gtcgagagcg cgtcgtaggc caccgccatc tcccggaaga
     1861 ggatgcccgt ggacagcgcg tcacccacga tgtggtgcat ggaggtaccg gcgacatgct
     1921 catcctctgt cagccggatc agcaccaccc gcagcatggg gccgcgcgcc agatcgaacg
     1981 gatggttgcc cacgcgccgc gccaggcgct tggcctcgcc gcggcgcgcc tccgtcggca
     2041 gggcatgaag atccaccagg gggagggacc actccaccga tggcgccacc ctctggaccg
     2101 gctcgccgcc cgcgacgtcg aaggtggtgc ggaggatctc gtgg
//
LOCUS       DASPJI010000353         8065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_485112, whole genome shotgun sequence.
ACCESSION   DASPJI010000353 DASPJI010000000
VERSION     DASPJI010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485112"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3170
                     /locus_tag="VEL74_09360"
     CDS             <1..3170
                     /locus_tag="VEL74_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62775.1"
                     /translation="VPLDPEYPRNRLAFMLDDLRQGQPAPVLVTHEWLVERLPGNEEA
                     RRPIFLGDSGEMDDLDDDLPGYVSVPVDPDDLAYLIYTSGSTGRPKGVALPHRALSNL
                     LAWHLGTLAGGVPILGFASLSFDVSFYEMFLAWSSGGTLVLAGEDERRDPAALDLLLR
                     SERVEKAVLPVVVLQQLAAEWDRQGGPPPELGEITTTGERLQITPAVRDLFQRSGGEA
                     RLHNHYGPAETHVATAWTLDPDPAAWDGFPPIGRPIANTTIHLLDAWGRPAPPGAAGD
                     LHIGGVCLARGYFGRPDLTAERFVPDAFAVSAGAPGGGRLYRTGDRARLRPDGAIEYQ
                     GRFDHQVKIRGFRVEPGEIEVALARHPAVAEAVVLPRDGKLVAYVVPRGNAAPDPGSL
                     ADALWEVVPSFMVPAAFVLLPSLPLNRNGKVDRRALPAPDAAATLEAGESGRRVAPRT
                     PAEEVVVAAWGEALGIDPAAISVHDDFFRLGGHSLLATRVVSRLRASFGVEVAVRVVF
                     EASTVEALARRVEELSREGRGVAPPPILPLPRPAPGGPPSEAPLSFAQEWMWLADRLD
                     PGGSAYLSPMPVRLRGRLDVPVLERSIHEVVRRHEMLRTTFPVSGSGPVQRVVPALEL
                     ELPMVDLQQVPRGEEEAARLLTVELRLLLDLERGPLLRLLLLRLGPEEHHLLLTAHHI
                     LFDGWSNGLLLAEVAALYGAFSRGEPSPLPELPIQYADFASWQRGWLQGETLERLLGY
                     WRRELEGELPVLRLPGARGAAVTLRSARQTVELGMELSAAIQAFARSRGTTVFAVLLA
                     ALQAHLHRLSGQDDILVGTPVAGRTRPEVEKLIGYFVNTLVMRGRLGGDPTFGDLLAQ
                     VWETALGAWAHQDLPFTRLVAELRPDRAASRTPLFQVLFLLQNAPMPAAALPGLEMEG
                     VNADVPIATFDLALSLKETPFGIAGWFFYNRDLFEPDAVARLAEGYRRLLEEALSAPE
                     RRLSELPVTGIAAAAAPVAGPQGAAAEEPAVPEPSGSRVADLSPEQRALLMLRLRRKA
                     RGEEAEVAEVAEVAVAASDRDNHGR"
     gene            3167..>8065
                     /locus_tag="VEL74_09365"
     CDS             3167..>8065
                     /locus_tag="VEL74_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62776.1"
                     /translation="MTEPRDIAGLSPEQRALLMLRLRRKAAREEEPGSAGIPRVPREG
                     PLPLSFAQQRLWFLDRWVPESPAYNIPAAVLLTGNLEVRALTGALTVIVQRHETLRTT
                     FPAVDGEPRQVIAPPGPLPVPLVDLTELPVDRGEEEGRRLGRLEARLPFDLARGPLLR
                     VRLLRLDRRKHLLLVNMHHIVSDGWSVGVLIRELTELYGACVEGRSPSLPPLPVQYAD
                     FAVWQRGRLQGEELERQLAYWRERLGDPPPPLELPTDRPRPAARTFEGGLRRFLFPEG
                     LWRAIEALCQHQGTTPFMVLLAGFAALLHRLTGQDGMTLGTPIANRNRQEIEGLIGFF
                     VNLLVLRADLAGDPPFGELLARVREETLGAYSHQDLPFEKLVEELQPERRLDAAPLFQ
                     VSFSLQNARLDRLELPGLGLEALPAETGATRFDLGLDIIDLDQTLGRWEYAAELFDPP
                     TLDRLSGHFCTLLVAATADPGLRISELPLLTEPERHQLLVGWNDTEAAYEKDLAIHEL
                     LEIQAAARPDAVAIVSATADNEQLTYAGLVRESRRLARHLRALGAGPDTVVGVSTERS
                     LRLPVAMLGILQAGGAYLPLDPTYPEARLALMREETGARIVLEDSGWAAARLSTDCLP
                     GGWSGAAPESLAYVLYTSGSTGRPKGVAIPHQGVLRLARGGGHARLDAGEVVLQVAPA
                     SFDASTLEIWGALLNGGRLVMMPPGRPTIDGLADTLVRHGVTTLLLTSGLFHAVVDER
                     LDALAGLRQIIAGGDVVSPTHVRRAAAALPGCDVWACYGPTEVTTFISCHPFWRRHLE
                     RSVPLGRPIADTQVYVLDRRLSPVPAGAVGELAAGGVALARGYLGRPDLTAERFVPDA
                     WGGEPGGRLYLTGDLVRYLPDGTLEFLGRADQQVKLRGFRVEPQEVEAVLLDHPAVAQ
                     AAVLVLGESESSRRLVAYVQGDGASGTREDLRAWLRERMPDFMVPSAVVAIPEMPLTF
                     HGKVDRRALASLAAVGPQADEETPFVPLRGPVEEVLGGIWAAVLKRERVGARDGFFDL
                     GGHSLLAIRVLSRVRRAFGIELPLRALFEEPTVAGLARRIERALDGSPPPPAIVPMAP
                     AARYRGLPLSFAQQRLWFIDQLEPDSAVYNTPFFLKLTGELDVSALAAAFAGAVERHE
                     VLRTTFAAAEEGSAFQVIGPPFAPDLPVIDVGGLSPRTRDAEARRLAALEAGRPFNLR
                     RGPVLRTSLLRLSPGEHVLLVNAHHIATDAWSMEILWREVALLYEGFRQERPAVLPPL
                     PVQYADFAVWQRGWLEGEVLESQLAYWRRRLGDEPAVVELPADRPRPAVQSYRGADLP
                     LALPRDASAGLAVLSRREGATPFMTLLALFVALLGRMTGQRDLPVGTAISGRRQPEVE
                     GLVGFFLNTLVIHAQLAEEATFRELVGQVRTLALEAYAHQDVPFEKLVDELRPRRSLS
                     HSPLFQVLFVLLHQAAGAEAVPRGLTAGPFPVERGTSKFDLMLALFSGGTEGDDFTGT
                     IEYATDLFDRSTIARLAEHLTTLASAVVAAPDAALSGLPLLAEPERHQLLAEWNDTAR
                     PLPDLPLHHLFERQAERTPDAPAASFGDHWLTYAELARRAARLARHLRRMGTGPESRV
                     GLGLDRSLEMVTGVLGVLTAEAA"
BASE COUNT          944 a         2948 c         2972 g         1201 t
ORIGIN      
        1 acgtccccct ggaccccgag tacccgcgca atcggctagc cttcatgctg gacgacctgc
       61 ggcagggaca gccggcgccg gtcctggtga cccacgaatg gctggtcgag cggctgccgg
      121 gcaacgagga ggcgcgccgc cccatcttcc tcggcgactc cggggagatg gacgacctgg
      181 acgacgacct acccgggtac gtatcggtcc cggtggaccc ggacgacctc gcttacctca
      241 tctacacatc agggtcgacc ggccgcccca agggggtcgc cctgccgcac cgggcgctct
      301 cgaacctcct cgcctggcac ctgggcaccc tggccggcgg cgtgccgatc ctgggcttcg
      361 cctcgctctc cttcgacgtc agcttctacg agatgttcct cgcctggtcc tccggcggca
      421 cgctggtcct ggccggcgag gacgagcggc gcgaccccgc ggccctggat ctcctcctgc
      481 ggtcggagcg ggtggagaag gcggtccttc ccgtggtcgt cctgcagcag ctcgcggcgg
      541 agtgggaccg gcaaggaggg ccgccgccgg agctggggga gatcaccacc acgggggagc
      601 ggctgcagat cacgcccgcc gtccgggacc tcttccagcg gagcggcgga gaggcgcggc
      661 tgcacaacca ctacgggccg gccgagaccc acgtggccac ggcctggacc ctggaccccg
      721 atccggcggc gtgggacggc ttcccgccga tcggccggcc gatcgccaac accaccatcc
      781 acctgctgga tgcctggggc cggccggctc ccccgggtgc cgcgggcgac ctccacatcg
      841 gcggcgtctg tctggcgcga ggctacttcg gccggccgga cctcaccgcc gagcgcttcg
      901 ttccggacgc cttcgcggtg tcggccgggg ctcccggggg aggacgcctc taccgcaccg
      961 gggaccgggc gcggctgcgg cctgacggtg ccatcgagta ccagggacgc ttcgaccacc
     1021 aggtgaagat ccgcggcttc cgggtggagc cgggggagat cgaggtggcg ctggcgcgcc
     1081 atccggcggt cgcggaggcc gtggtcctgc cgcgggacgg caagctggtg gcctacgtgg
     1141 tgccgcgggg gaacgccgct ccggaccccg gcagcctggc cgacgcgttg tgggaggtcg
     1201 tcccgtcctt catggtgccg gcggcgttcg tcctcctgcc gtcgctgccg ctgaaccgca
     1261 acggcaaggt ggaccgccgg gcgctccccg ctccggatgc cgcggcgacg ctggaggccg
     1321 gagagtccgg ccggcgggtg gctccccgca ccccggcgga ggaggtggtg gtggcggcgt
     1381 ggggcgaggc gctggggatc gatccggccg ccatctcggt gcacgacgac ttcttccgtc
     1441 tgggcggcca ttcgctcctc gccacgcggg tggtctcccg gctgcgcgcc tccttcggcg
     1501 tcgaggtggc ggtgcgcgtg gtcttcgagg cctccaccgt cgaggcgctg gcgcgacggg
     1561 tggaggagct gtcgcgcgaa gggcgcggtg tggcgccgcc tcccatcctc cccctcccac
     1621 ggcctgctcc cggggggccg ccctccgagg cgccgctctc cttcgcccag gagtggatgt
     1681 ggctcgctga ccgtctcgat ccggggggct cggcttacct gtcgcccatg cccgtccgct
     1741 tgcgtggacg tctggacgtg ccggtgctgg agcgcagcat ccacgaggtg gtgcggcgcc
     1801 acgaaatgct gcgcaccacc ttcccggttt ccgggagcgg gccggtgcag cgcgtcgttc
     1861 cggcgctcga gctggagctt ccgatggtgg acctccagca ggtgccgcga ggggaggagg
     1921 aggcagcccg gctgctgacg gtcgagctgc ggctccttct cgacctcgag cgcgggccgc
     1981 tcctccgcct cctgctgcta cgcctcgggc cggaggagca ccacctgctg ctgaccgcgc
     2041 accacattct gttcgatggc tggtccaacg gcctgctgct cgccgaggtg gcggcgctct
     2101 atggcgcctt ctcgcgcggc gagccgtcgc cattgccgga gctgccgatc cagtacgccg
     2161 acttcgcctc ctggcagcgc ggctggctgc agggggagac gctggagcgg ctgctcggct
     2221 actggcggcg ggagctggaa ggggagctgc cggtgctgcg gctgcccgga gcccgcgggg
     2281 cggccgtcac cctgcgaagc gcgcggcaga ccgtggagct ggggatggag ctgtcggccg
     2341 ccatccaggc ctttgcgcgg agccggggga ccaccgtgtt cgcggtgctc ctcgccgccc
     2401 tccaggcaca tctccaccgg ttgagcgggc aggacgacat cctggtgggg actccggtgg
     2461 ccggccgcac ccggcccgag gtggagaagc tcatcggcta cttcgtcaac accctggtga
     2521 tgcgcggccg gctcggcggc gatcccacct tcggcgacct cctggctcag gtctgggaga
     2581 cggcgctcgg tgcctgggcg caccaggacc tgccgttcac ccggctggtg gccgagctgc
     2641 ggccggaccg cgccgccagc cgcacacccc tgttccaggt cctgttcctg ctccagaacg
     2701 cgccgatgcc ggcggcggcg ttgcccggcc tggagatgga gggggtgaac gccgacgtcc
     2761 ccatcgccac cttcgacctc gccctgagcc tcaaggagac gccgttcggc atcgccggct
     2821 ggttcttcta caaccgcgac ctcttcgagc cggacgcggt cgcccgcctc gccgaggggt
     2881 accggcggct cctcgaggag gcgttgtccg cccccgagcg ccggctctcg gagctgccgg
     2941 tgacagggat cgcggccgcc gcggcaccgg ttgccggccc gcagggtgcc gcggcagagg
     3001 agcccgctgt gccggagccg tccgggagtc gcgtggcgga cctctctccc gagcagcggg
     3061 cgctcctcat gctgcgcctg cggcgcaagg cgcgggggga ggaggccgag gtggccgagg
     3121 tggccgaggt ggccgtggcg gcctccgaca gggataatca cggccgatga cggagccgcg
     3181 ggacatcgcc ggactctctc ccgagcagcg ggcgcttctg atgctgcgcc tgcggcgcaa
     3241 ggcggcgcgg gaagaggagc cggggtcggc cggcatcccc cgcgtgccgc gcgaggggcc
     3301 cctgccgctg tcctttgccc agcagcggct ctggttcctc gaccgctggg tgcccgagag
     3361 cccggcgtat aacatcccgg ccgccgtcct cctcaccggg aacctcgagg tgcgggcgct
     3421 gaccggcgcc ctcacggtca tcgtccagcg ccacgagacc ctgcgcacca cgtttccggc
     3481 cgtggacggc gagccccgcc aggtgatcgc gccgcccggc ccgttgccgg tgccgctcgt
     3541 cgacctgacg gagctcccgg tcgacagggg agaggaggag gggcggcggc tcggccggct
     3601 cgaggcccgt ctccccttcg acctcgcgcg cggcccgctg ctccgcgtcc gtctcctgcg
     3661 gctggaccgg cgcaagcacc tgctgctggt caacatgcac cacatcgtct ccgacggctg
     3721 gtcggtgggg gtgctgatcc gtgagctgac ggagctctat ggcgcgtgcg tggaagggcg
     3781 ctcgccgtcc ctgccgccgc tgccggtgca gtacgccgat tttgccgtct ggcagcgcgg
     3841 ccggttgcag ggcgaggagc tggagcgcca gctcgcctac tggcgcgagc ggctcggcga
     3901 tccccctccg ccgctggagc tgcccaccga ccggccgcgt ccggcggccc gcacgttcga
     3961 gggggggctg cggcggttcc tcttccccga aggcctgtgg cgggccatcg aggccctctg
     4021 ccagcaccag ggaacgacgc cgttcatggt gctgctcgcc ggcttcgcgg cgctgctgca
     4081 tcgcctcacc ggccaggatg ggatgaccct ggggacgccc atcgccaacc ggaaccgcca
     4141 ggagatcgag gggctgatcg gcttcttcgt caacctcctg gtgctgcggg ccgatctcgc
     4201 gggcgatccc cccttcggcg agctgctcgc ccgggtgcgc gaggagactc tgggcgccta
     4261 ctcccaccag gacctgccgt tcgagaagct ggtggaggag ctgcagcccg aacgccgcct
     4321 ggacgcggcg ccgctgttcc aggtgtcgtt ctcgctccag aacgcgcggc tggaccggct
     4381 ggagctcccc gggctggggc tggaagcgct gccggccgag accggcgcga cccgtttcga
     4441 cctcgggctc gacatcatcg atctcgacca gacgctgggg cgctgggagt acgcggccga
     4501 gttgttcgac ccgcccacgc tcgaccggct gtccgggcat ttctgcaccc tgctcgttgc
     4561 ggctacagcc gatcccggcc tccggatctc cgagctgccc ctgctcaccg agcccgagcg
     4621 tcatcagctc ctggtcggct ggaacgacac cgaggcggcc tacgagaagg acctcgccat
     4681 ccacgagctg ctcgagatcc aggcggcggc gaggccggat gccgtggcca tcgtttcggc
     4741 caccgctgac aatgagcagt tgacctatgc cggcctggtc cgggagtcgc ggcgcctcgc
     4801 ccgccacctc cgggcgctcg gagccgggcc cgacaccgtg gtgggggtct cgaccgagcg
     4861 ctccctccgc ctgccggtgg ccatgctggg catcctccag gccggaggcg cctatctccc
     4921 actcgatccc acctatcccg aggcgcggct ggcgctgatg cgcgaggaga ccggagcgcg
     4981 gatcgtgctc gaggattcag gctgggcggc ggcccggctg agcacggatt gcctgcccgg
     5041 cggatggagc ggtgcggcgc cggagagtct ggcctacgtc ctctacacct ccggctccac
     5101 cggccggccc aagggggtgg cgatcccgca ccagggcgtg ctgcggctgg cgcgcggcgg
     5161 cggccatgcg cggctcgatg ccggcgaggt ggtgctgcag gtcgcgccgg cctccttcga
     5221 tgcctccacg ctcgagatct ggggggcgct cctcaacggc ggcaggctgg tgatgatgcc
     5281 gccgggccgg ccgacgatcg acggtctcgc cgacacgctg gtacgccacg gggtcaccac
     5341 gctcctcctc acctcgggac tcttccacgc ggtggtggac gagcgcctcg acgccctggc
     5401 cggcctgcgc cagatcatcg ccggcggcga cgtggtctcg cccacccacg tgcgccgggc
     5461 cgccgccgcc ctccccggct gcgacgtctg ggcctgctac ggtcctaccg aggtcaccac
     5521 gtttatctcc tgccacccct tctggagaag gcatttggag aggagcgtcc cgctcgggcg
     5581 ccccatcgcc gacacccagg tctacgtgct ggaccgccgg ctctcgccgg tgcccgccgg
     5641 agccgtgggg gagctggcag ccggcggcgt cgcgctggcc cggggctacc tggggaggcc
     5701 cgacctcacg gccgagcgct tcgtgcccga cgcctggggc ggcgagccgg gggggcggct
     5761 ctacctcacc ggcgacctgg tgcggtacct gccggacggc accctggagt tcctgggccg
     5821 ggccgatcag caggtgaagc tgcggggatt ccgtgtcgag ccccaggagg tggaggcggt
     5881 gctcctcgat cacccggcgg tcgctcaggc cgcggtgctg gtgctggggg agagcgagag
     5941 cagccggcgc ctggtggcgt acgtccaggg cgatggagcc agcgggacgc gggaggacct
     6001 ccgggcctgg ctgcgggagc ggatgccgga cttcatggtg ccgtcggcgg tggtggccat
     6061 ccccgagatg ccgctcacct tccacggcaa ggtggaccgg cgggcgctgg ccagcctggc
     6121 ggcggtgggt ccgcaggcag acgaggagac ccccttcgtg ccgctccgcg ggccggtgga
     6181 ggaggtgctc ggggggatct gggccgcggt cctgaaacgc gagcgggtcg gagcgcggga
     6241 tggcttcttc gacctcggcg ggcactcgct cctggcgatc cgggtgctct cgcgcgtccg
     6301 ccgggcgttc gggatcgagc tgccgctacg cgctctgttc gaggagccga cggtggcggg
     6361 gctcgcgcgg cggatcgagc gcgccctgga cggctcccct ccgccgccgg cgatcgtgcc
     6421 catggcgccg gcagcgcgct accgcggcct gccgctctcc ttcgcccagc agcggctctg
     6481 gttcatcgac cagctcgagc cggacagcgc ggtctacaac acgccgttct tcctgaagct
     6541 gaccggcgag ctggacgttt cggcgctcgc cgcggccttc gccggggcgg tcgaacggca
     6601 cgaggtgctg cgcaccacct tcgccgccgc agaagagggc tccgccttcc aggtgatcgg
     6661 accccccttc gctccggacc tgccggtgat cgacgtggga ggcctctccc cgcggacccg
     6721 cgacgccgag gcgcgccggc tggcggcgct ggaggccggg cgccccttca acctgcggcg
     6781 cggcccggtc ctccgcacct cgctgctgag gctgtcgcct ggcgagcacg tcctcctggt
     6841 caatgcccat cacatcgcca ccgacgcctg gtcgatggag atcctgtggc gcgaggtggc
     6901 gctcctctac gagggattcc ggcaggagag gcccgccgtc ctgccgcccc tgccggtcca
     6961 gtacgccgac ttcgcggtct ggcagcgcgg gtggctggaa ggcgaggtgc tggagtccca
     7021 actcgcctac tggcgccgcc gcctcggcga tgagccagcg gtcgtggagc tgccggcgga
     7081 ccggccgcgg ccggcggtgc agagctaccg cggcgccgac ctgccgctgg cgctcccgcg
     7141 cgacgcctcg gcggggctcg cggtgctgtc ccggcgcgag ggggcgactc ctttcatgac
     7201 cctgctggcc ctgttcgtcg cccttctcgg gcggatgacg gggcagcggg acctgccggt
     7261 cgggacggcg atctcgggcc gccggcagcc ggaggtcgag gggctggtcg gcttcttcct
     7321 gaacaccctg gtgatccacg cccagttggc ggaggaggcc acgttccgcg agttggtggg
     7381 gcaggtgagg acgctggcgc tcgaagccta tgcccaccag gacgtcccct tcgagaagct
     7441 ggtggacgag ctgcgcccgc ggcgcagcct cagccactca ccgctcttcc aggtgctctt
     7501 cgtcctgctg caccaggccg cgggcgcgga ggccgtgccg cgagggctca cggccggccc
     7561 gtttccggtc gagcgtggga cctccaagtt cgacctcatg ctcgcgctgt tctccggagg
     7621 caccgagggc gacgacttca ccggtacgat cgagtacgcg accgacctct tcgaccgctc
     7681 gaccatcgcc cgtctggcgg agcacctgac gactctggcc tcggcggtcg tggcggcgcc
     7741 ggacgcggcg ctctccggcc tgcccctgct cgccgagccc gagcggcacc agctcctggc
     7801 cgagtggaac gacaccgcgc gcccactacc ggacctgccg ctccaccacc tcttcgagcg
     7861 gcaggcggag cggacgccgg acgccccggc ggctagcttc ggcgaccact ggctgaccta
     7921 tgccgagctg gcgcgccggg cggcgcggct cgcccgccat ttgcggcgga tgggaaccgg
     7981 ccccgagtcg cgggtggggc tgggcctgga ccgctcgctc gagatggtga ccggcgtcct
     8041 cggcgtcctc acggctgagg cggcc
//
LOCUS       DASPJI010000354         6526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_829892, whole genome shotgun sequence.
ACCESSION   DASPJI010000354 DASPJI010000000
VERSION     DASPJI010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829892"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>6526)
                     /locus_tag="VEL74_09370"
     CDS             complement(<1..>6526)
                     /locus_tag="VEL74_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62777.1"
                     /translation="RPPLLSDRGASLALRLGPETVGRLRELGRSQGTTLFTVLLTAFQ
                     TLLHRHSGQLDVLVGSPAAGRAHRRFADLVGYLVQPVVLRADFTGDPSATDLLRRTRQ
                     SVAGALEHQDYPFPLLVERLQPERQAGRSPLFQAMFVLYPGQPAGGRLAGLAAAVLGE
                     EGARLDVGGLELESLSLGESRVQLDLTLAMAEISETGEDGENGGGLLASFQYSRDLFD
                     ASSIALMGGHLEALLAGLSEDAGRPVGDLPLLTLAERRQLLAWNAPAETAPDACLHQL
                     FEAQATRTPEAEALVAGGERLTYGELDRRAGRLAQRLRAQGVGPESVVGVYLERSAGL
                     VTALLAVLKAGGAYLPLDPAYPGERLGLLLEDAGAAVLVTREPLLQRLPRHPGRVVLV
                     EGGEPAAGSEPGAIDPGSARAVLPGDLAYLIYTSGSTGHPKGVAIAHRNAVALVRWAR
                     RTFEERELAGVLASTSAAFDLSVFELFVPLANGGRVILAANALELASLAAASEVTLVN
                     TVPSAIAELVELRALASGELTVNLAGEALTPDLVRRLHGQPAVRRLLNLYGPSETTTY
                     STFAVLPHGEEGIPPIGHPVAGTRVYLLDAGLRPVPSGAAGEVYIGGDGVSRGYHARP
                     ALTAERFVPDPFSPVPGERLYRTGDLARRRPDGSLVYLGRIDDQVKIRGFRVEPGEVQ
                     AALAAHPEVREAVVLALAGAGGERRLTAWVVPSGGAAPSTADLRRYLRERLPDSMVPA
                     DFVFLASLPLTINGKVDRRALAVMSTGLPAAGRPESAGGPRDPRDPITEVLAGLWADL
                     LRAERVGIHDGFFELGGHSLLAARVVARVREAFGVELPLSWVFAQPTVAELAAEVRRV
                     RDAGIAGEAPLLLLEQRSEPRPAEIPLSFAQERLWFLDRLVPGQAVYNIPVAARLRGR
                     LDPALLQRALDGLLARHESLRTVFADGSTGPVQRVLPAAPFPLEMADLELDTEPEDEA
                     WRRAAAEVRRPFDLATGPVVRGLLLRLAPEDHLLVLTLHHIAADGWSIGVLFRDLEAL
                     YRPAGEEPALPPLPVQYADYALWQRGWLTGEALDRRLGWWREALAGAPEALELPADRP
                     RPPAPSWRGTAVPFALPAPTVARLREVARRQDATLFMAVLAGFGALLSRLSGQTDGVA
                     GTMMANRDRSELDGVAGFFVNALPLRLRLNGAPGLGALLGPTREMALGAFDHGDLPFE
                     RLVEALAPRRASSQDGSRGALFQVCLSFEETPARAPRLPGLAVELAPLHTGTSRFDLT
                     LLLESDGGGLSGGAEASADLFDTATVERMLHHLSTLLGGAAADPERPLDDLPLLSPAE
                     RRQAIVEWNRPAYEVPAGAAEATLTGLFAAAAARSPEAVAVTGDGGDGHHLTYAGLDR
                     AANRLAHRLRRLGVGPETRVALFVERSPELVVGLLGILKAGGAYVPVDPEVAGERLAP
                     ILADSRPRALVTQGSLAHRLPALDDGPVIVRVDDPGLAQGDQGDDAAPADLTAPSHAA
                     YVIYTSGSTGRPKGVVVEHGQVARLLAATDGFFGFGPGDVWSLFHSIAFDFSVWEVWG
                     AMARGGRLVVVPYWTSRSPAELSALIAAEGVTVLNQTPSAFRQLFHAEGQSLAAPRPP
                     LRWVIFGGEALEPAALAPWFERHGDVRPRLVNMYGITETTVHVTFRPLSRDDAGTGGR
                     PAPSLLGRPIPDLRIYVLDPRGEPVPLLAAGEIHVGGAGVARGYLEQPALTAEAFVPD
                     PFSGRSGARLYRSGDLARRLPGGDLEYLGRRDHQVKIRGFRIELAEIEAALAAVPGVR
                     EAVVVARGEGLEAEPGDRRLVAYVVGDVAAGELSRSLRERLPGYMVPATFVPLPALPL
                     TPNGKVDRKALPAPERQGPKGGHVAPRTPAEEILAGIWAETLGLERVGVADDFFGLGG
                     HSLLATRVMSRLREAFDVEMPLRDLFEAPRLEDFAARVEAARWSGVGRVLPPLVPRAP
                     ELRGEPLPLSFAQQRLWLIDQLEPGRPLYNIAVALRAEGPLDGGVLARCLGEIVRRHE
                     ALRTVFTGLDGSPVQVIEPAAPFTLPVDDLSGLPVSQREATALARVGEEAGRPFDLTL
                     SPMLRGLLLRLGPEDQIVALTLHHIAGDGWSMGILVRELTALYAAFAEGGPSPLPELP
                     VQYADFSVWQRSWLDGEALESEVAYWRRQLTGLPPRLELPTDR"
BASE COUNT          951 a         2440 c         2441 g          694 t
ORIGIN      
        1 cggtcggtgg ggagctcgag ccgtggcggc aggcccgtga gctgccggcg ccagtaggcg
       61 acctcgctct ccagcgcctc gccatccagc caggagcgtt gccataccga gaagtccgcg
      121 tactgcaccg gcagctccgg caggggcgat ggcccgccct cggcgaaggc cgcgtacagg
      181 gccgtgagct cccgcaccag gatccccatc gaccagccgt cgccggcgat gtgatgcagg
      241 gtcagcgcga cgatctggtc ttccggtccg agccgcagca gcaggccgcg cagcatcgga
      301 ctgagggtga ggtcgaacgg gcggccggcc tcttcgccca cccgggcgag ggccgtcgcc
      361 tcgcgctggc tcaccggcaa tcccgacagg tcgtccaccg gcagtgtgaa gggcgccgcc
      421 ggctcgatca cctgcaccgg cgagccatcc aacccggtga acaccgtgcg caacgcctcg
      481 tgccggcgca cgatctcgcc gaggcaacgt gccagcacgc cgccgtccag cggcccctcg
      541 gcgcgtagcg ccaccgcgat gttgtagagc ggccggcccg gctcgagctg gtcgatgagc
      601 cagaggcgct gctgagcgaa ggagagaggc agcggctcac cccgcagctc cggagcgcgg
      661 gggaccagcg gcggcaggac ccggcccacc ccggaccatc gcgccgcctc gacccgggcg
      721 gcgaagtcct ccagccgggg agcctcgaac agatcgcgca gcggcatctc gacgtcgaag
      781 gcctcccgca ggcgcgacat cacccgcgtc gccagcagcg aatgcccccc gaggccgaag
      841 aagtcgtcgg ccaccccgac ccgctcgagg ccgagggtct cggcccagat gccggcgagg
      901 atctcctccg ccggcgtcct cggcgccaca tggcctccct ttgggccctg ccgctccggc
      961 gccggcaggg ccttgcggtc cactttgccg ttcggggtga ggggcagggc cgggagcggc
     1021 acgaaggtgg ccggcaccat gtagcccggc aggcgctcac gcagtgaccg gctcagctcg
     1081 ccggcggcaa cgtcgcccac cacgtaggcg accagccgcc ggtcgccggg ctccgcctcc
     1141 agtccctccc cccgcgccac cacgaccgcc tcgcggaccc ccggcacggc ggccagcgcc
     1201 gcctcgatct ccgccagctc gatgcggaag ccgcggatct tgacctggtg atcgcggcgg
     1261 cccaggtact cgaggtcgcc gccgggcagg cggcgggcga ggtcgccgga gcggtagagg
     1321 cgggcgccgg agcggccgga gaaggggtca ggaacgaagg cctccgccgt gagcgcgggc
     1381 tgctccaggt agccccgggc gacgccggca cccccgacgt ggatctcgcc ggcggcgaga
     1441 agaggcacgg gctcgccccg gggatcgagc acgtagatcc gcaggtccgg gatcggccgg
     1501 ccgaggaggc tcggcgccgg ccgccctccc gtccccgcgt cgtcccggga gagagggcga
     1561 aaggtgacgt gcaccgtggt ctcggtgatg ccgtacatgt tcaccagccg cggccgcacg
     1621 tcgccgtggc gctcgaacca gggggcaaga gccgccggct ccagggcttc gccgccgaag
     1681 atcacccagc gcaggggcgg ccgcggggcg gccaggcttt gcccctcggc atggaaaagc
     1741 tgccggaagg ccgagggcgt ctggttgagc accgtcaccc cctcggcggc gatcagcgcg
     1801 gagagctcgg ccggcgagcg gctcgtccag tacggtacga ccaccagccg gccgccccgg
     1861 gccattgccc cccagacctc ccagaccgag aagtcgaagg cgatggagtg gaacaggctc
     1921 cagacatctc ccggaccgaa gccgaagaag ccgtccgtgg ccgccagcag acgggccacc
     1981 tggccgtgct cgacgacgac gcccttcggg cggccggtgg agccggacgt gtagatcacg
     2041 tacgccgcgt ggctaggcgc agtgaggtcg gccggcgccg cgtcgtcgcc ctgatcgccc
     2101 tgcgccaggc cagggtcgtc gacgcgaacg atcaccggcc catcgtccag cgccggcagc
     2161 cggtgcgcca gggagccctg ggtgaccagg gcgcgcgggc gggagtcagc gaggatgggg
     2221 gccaggcgct cccccgccac ctccggatcg accggtacgt acgcgccgcc agccttgagg
     2281 ataccgagca ggcccacgac caactcggga gaccgctcga cgaagagggc gacccgcgtc
     2341 tccgggccga cgccgaggcg gcgcaggcgg tgggccagcc ggttggcggc gcggtccagc
     2401 ccggcatagg tgaggtggtg cccatccccg ccgtctccgg tgacggccac cgcttcgggc
     2461 gaccgcgccg cggcggcggc gaagaggccg gtgagggtcg cttcggccgc gccggccggc
     2521 acctcgtacg ccggccggtt ccactccacg atcgcctgcc ggcgctccgc cgggctcagc
     2581 agcggcaggt cgtccagcgg gcgctcggga tcggcggccg ctccgccgag gagggtcgac
     2641 aggtgatgga gcatgcgctc caccgtggcg gtgtcgaaga ggtccgcgct ggcctcggcg
     2701 ccgccggaga gcccgccgcc atcgctctcg aggagcagcg tcaggtcgaa gcgcgaggtg
     2761 ccggtatgga gcggcgcgag ctccaccgcc aggccgggga gccgcggcgc gcgggccggc
     2821 gtctcctcga agctcagaca gacctggaac agcgcgcccc gggacccgtc ctgagaggag
     2881 gcgcgccgcg gcgccagggc ctccaccagg cgctcgaagg ggaggtcgcc gtggtcgaag
     2941 gcgccgaggg ccatctcccg cgtcgggccg agcaacgccc ccaggccggg cgcgccgttc
     3001 aggcgcagac gcaggggcag ggcgttgacg aagaagccgg ccacaccgtc gagctcggac
     3061 cggtcgcggt tcgccatcat agtgccggcg acgccatcgg tctggccgct cagacgggaa
     3121 agcagggcgc cgaagccggc gaggacagcc atgaacagcg tcgcgtcctg gcgccgggcc
     3181 acctcccgca ggcgcgccac cgtgggcgcc ggcagggcga agggcaccgc cgtcccgcgc
     3241 cagctcggcg caggaggccg cggccggtcc gccggcagct ccagggcttc gggggcgccg
     3301 gccagggcct cacgccacca gccgagccgg cggtccaggg cctcgccagt cagccagccg
     3361 cgctgccaca gggcgtagtc cgcgtactgg acgggaagcg gcggcaaagc cggctcctcg
     3421 ccggccggcc ggtagagggc ctccagatcg cggaacagga cgccgatgga ccagccgtcg
     3481 gcggcgatgt ggtggagcgt gagcaccagc agatggtcct ccggcgcgag gcgcaggagg
     3541 aggccgcgga cgaccggccc agtggccagg tcgaagggcc ggcggacctc cgcggcggcg
     3601 cgacgccatg cctcgtcctc cggctcggtg tcgagctcca ggtcggccat ttccaggggg
     3661 aacggcgcgg ctggcaggac gcgctgcacc ggccccgtgc tcccgtcggc gaagaccgtc
     3721 cgcagggact cgtggcgggc cagcagaccg tccagcgcgc gctggaggag cgcggggtcg
     3781 aggcgtcccc gcaggcgcgc ggccacgggg atgttgtaga ccgcctggcc gggcaccagc
     3841 cggtcgagga accagagccg ctcctgcgcg aaggagaggg ggatctcggc cggccgcggc
     3901 tccgaccgct gctccagcag caagagcggc gcctcaccgg ctatgcccgc gtcacggacg
     3961 cgcctcacct cggcggccag ctcggccacg gtcggctgcg cgaagaccca ggagagcggc
     4021 agctccacgc cgaacgcctc gcgcacccgc gccaccaccc gggccgccag cagcgagtgg
     4081 ccgcccaatt cgaagaagcc gtcgtggatc cccacccgct cggcccgcag caggtcggcc
     4141 catagtccgg ccagcacctc cgttatcgga tcccgcggat cccgcggccc gccggcgctc
     4201 tccggccggc cggcggccgg cagcccggtg ctcataacgg ccagcgcccg ccggtccacc
     4261 ttgccgttga tcgtcagcgg cagggacgcc aggaagacga aatccgccgg caccatcgaa
     4321 tcgggcaggc gctcgcgcag gtaccgtcgc aggtcggcgg tggacggggc ggcgcccccg
     4381 gaaggcacca cccaggcggt caggcgccgc tctccgcccg cgccggccag ggcgaggacc
     4441 acggcctcgc gcacctccgg atgggccgcg agagccgcct ggacctcccc gggctcgacc
     4501 cggaagccgc ggatcttgac ctggtcgtcg atgcgtccca ggtacacaag cgagccgtcc
     4561 ggccgccggc gggcgaggtc gccggtccgg tacagacgct cgcccggtac gggcgagaag
     4621 ggatccggga cgaagcgctc ggccgtcagg gcgggccgcg cgtgatagcc gcgcgacacc
     4681 ccgtcgccgc cgatgtagac ctcgccggcg gcgccggagg gcaccggccg cagcccggcg
     4741 tcgagcaggt acacgcgggt gcccgcgaca ggatggccga tcggcgggat cccctcctcg
     4801 ccgtgcggca ggaccgcgaa ggtcgagtag gtcgtggtct ccgaggggcc gtagaggttg
     4861 agcagccgcc ggaccgcggg ctgcccgtgg agccgccgca ccaggtccgg ggtcagcgcc
     4921 tcgcccgcca ggttcaccgt cagctcgcca gaggcgaggg cccggagctc gaccagctcg
     4981 gcgatggcgg acggcacggt gttgaccagg gtcacctcgg acgcggcggc cagcgaggcg
     5041 agctccaggg cgttggcggc gaggatcacc cggccgccat tggccagggg gacgaacagc
     5101 tcgaagaccg agaggtcgaa ggcggcggac gtcgacgcca gcacgccggc gagctcccgc
     5161 tcctcgaatg tccggcgcgc ccagcgcacc agtgccacgg cgttccggtg cgcgatggcg
     5221 acccccttgg ggtgcccggt ggagccggag gtatagatca gataggcgag gtcgcccggc
     5281 aggacggccc gcgcggatcc aggatcgatg gctccaggct cgctcccggc ggccggctcc
     5341 cccccctcca ccagcaccac ccggcccggg tggcgcggca gccgctgcag cagcggctcg
     5401 cgggtgacca gcaccgccgc gcccgcgtcc tccagcagca ggcccagccg ctccccggga
     5461 taggccgggt ccaggggcag gtaggcccct cccgctttca gcaccgccag gagcgccgtc
     5521 accaggccgg ccgagcgctc caggtagacg ccgaccaccg actcggggcc tacgccctga
     5581 gcgcgcagcc gctgggccag ccggccggcc cgccggtcca gctcgccata ggtcaaccgc
     5641 tcgccgccgg ccaccagcgc ctccgcctcc ggggtccgcg tggcctgggc ttcgaacagc
     5701 tggtgcagac aggcgtccgg cgccgtctcc gcgggggcgt tccaggccag gagctggcgc
     5761 cgctcggcaa gggtaagtag cggcaggtct cctacgggcc ggccggcgtc ctcgctgagg
     5821 ccagcgagca gggcctccag gtgcccgccc atcagcgcga tggaggaggc gtcgaacaga
     5881 tcgcggctgt actggaagga ggccagcagg ccgccgccgt tctcgccgtc ctcgccggtc
     5941 tcgctgatct cagccatcgc cagagtcagg tcgagttgca cgcggctctc gcccagggag
     6001 agggactcga gctccagccc gccgacgtcg aggcgcgccc cctcctcgcc gaggacggcc
     6061 gcagccagtc cggcgagccg cccaccggcg ggttgccccg gatagaggac gaacatcgcc
     6121 tggaacagcg gcgacctgcc cgcctggcgc tccggctgca gccgctcgac cagcaacggg
     6181 aagggatagt cctggtgctc cagcgctccc gccacggatt gccgcgtgcg gcgcagcagg
     6241 tcggtggccg aagggtcgcc ggtgaagtcg gcccgcagga ccacgggctg gacgaggtag
     6301 cccaccaggt ccgcgaagcg gcggtgggct cggcccgcag cgggcgatcc caccagcacg
     6361 tcgagctgac ccgagtgccg gtggagcagc gtctggaacg cggtgaggag cacggtgaag
     6421 agcgtcgtcc cctgggagcg gcccagctcg cgcaggcggc ccacggtctc cggccccaag
     6481 cgcagcgcca gcgaggcgcc acggtccgag agcagcggcg ggcgcg
//
LOCUS       DASPJI010000355        20683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_366341, whole genome shotgun sequence.
ACCESSION   DASPJI010000355 DASPJI010000000
VERSION     DASPJI010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 20683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20683)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20683
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366341"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /locus_tag="VEL74_09375"
     CDS             complement(<1..1711)
                     /locus_tag="VEL74_09375"
                     /inference="COORDINATES: protein motif:HMM:NF020928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HYG62778.1"
                     /translation="MSKSAVLESRFVEIIASEVGCRPQQVAAASDLFAEGATVPFVAR
                     YRKEATGGLEDLQLETVFKRREYFLELAERRDAILDSIREQGKLTGELEAAIRAAVGK
                     QELEDLYLPYKPKRRTRAQIAREKGLEPLAEAVLAAATGSTPADELARPFIDPEKGVE
                     DAAAALAGARDILAERLSENAEHRAELRRVLANEGRLTVAVMPGKEKDPEALVYRDYF
                     DHQEAGKDIPSHRLLAILRGEREGFLLSDLRIEDEREVGHLAMSWGVPVDTPCGREVV
                     AATADGYKRLLRPSITNEVRSEMRERAEAQAIAVFRANLEALLLQAPLGQYAVMGLDP
                     GYRTGCKMAVVDPTGRVVATDVIHAIPPHADEARAAAIVVGLIRQHAVKAVAVGNGTA
                     SRETEAFARKAVREAGIEEVVVAIVPETGASVYSASAVAREELPQLDVSLRGAVSIAR
                     RLQDPLAELVKIEPRSMGVGQYQHDVDQKALERELDTAVEGAVNRVGVELNTASAPLL
                     RRVSGLSERLARAVVEHRDKNGPFRSRKSLLSIAGFGPKTFELSAGFLRIRGAEHPLD
                     STAV"
     gene            complement(1808..2104)
                     /locus_tag="VEL74_09380"
     CDS             complement(1808..2104)
                     /locus_tag="VEL74_09380"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYG62779.1"
                     /translation="MKRSLSLAPLAKKELDEAVDYYDAESPGLGRAFVSEIEDAFEQI
                     LDYPESAPIVRGTVRRKILRRFPYDLLYSVRPDSVRILAVMNQKRRPFYWWGRK"
     gene            complement(2101..2325)
                     /locus_tag="VEL74_09385"
     CDS             complement(2101..2325)
                     /locus_tag="VEL74_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HYG62780.1"
                     /translation="MNLADIEAEALKLNPNGRAKLATTLLHSLEDLSEAEIENLWFEE
                     AERRDRELDEGGATAIPAEEVLREIRARLR"
     gene            2418..3029
                     /locus_tag="VEL74_09390"
     CDS             2418..3029
                     /locus_tag="VEL74_09390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG62781.1"
                     /translation="MIDPIEAVHLGDEEQPDDAELMARVRTGDRHAFARLVDRHKDAV
                     VNYVARLTGSRDRAEDLAQETFLRLFRAAAGYLEQGHLRAYLYRIATNLVRSEERRER
                     RLRLLLPFLGPDRPHAEPAAPSGLLRQEIHRKVAAAVAELPLRFRVPLVLHEIEGWPY
                     ADIARELGCREGTVKSRIHRGRQQLKQELEPYWNGGTPWKINR"
     gene            3011..3442
                     /locus_tag="VEL74_09395"
     CDS             3011..3442
                     /locus_tag="VEL74_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62782.1"
                     /translation="MEDQPLGRLLRELPRERAGDRFTARVLEGLDGGRRPARRSGWAL
                     ATAALLALLVSGVLVERRGLRQGEPAHSEAGEARQALEEIRDEHERLARELERLQSLS
                     EGQGDAPGIVYLGGNERMDLVLDLGRMPEGTLPASYGSETF"
     gene            3471..4214
                     /locus_tag="VEL74_09400"
     CDS             3471..4214
                     /locus_tag="VEL74_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HYG62783.1"
                     /translation="MNSRALFRKTAWALALGLVTLAPAVSANAGADQGDDKEKKVEKR
                     RIVIVDKNGERQVIEGEGPSVRRGYLGVGLTDLTPELRAHFGASESAGVMVSSVEPGS
                     PADKAGVKVGDIITSFDRDAVKSSWDVRSKVRGYEDGQQVALEVVRDGRAQSFTASMV
                     QRERPEFDVTPFFFGKDGKVDPKLFHFEGDLRNIETIQIPSWDKEGGPGGPGPRVQVL
                     RSREVELEKQLQKLEKRIAELEKQLDRKN"
     gene            complement(4281..6395)
                     /locus_tag="VEL74_09405"
     CDS             complement(4281..6395)
                     /locus_tag="VEL74_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HYG62784.1"
                     /translation="MTTTALLQPRAFRNPFHTPRERLLRYAFMKLTRDLDSRFEALLL
                     TGRVSKWYSEVGNEATTIPAGLALESGDALCTLHRDLGAILPVYLDPARAFPGLGFGD
                     PDGRRPDPERILYRLACQLLGKADGFSQGFERSFHYGYLAPENGILHLGMISHLGSMI
                     PVAAGCGFAFRQRGTDRVAINFIGDGATSTGDFHEGLNMAAVWKLPLILIIENNRYAF
                     STPAHLQYAAKRLSDRGPGYGIPAETVDGNDPDAMAEVLDRAVARARAGHGPTLIEAM
                     LGRMRGHSEGDDSLKVVPADELAAYRAADPVPAYAKRLEEEGVLDAGLRARLEARTAE
                     LVERAISSALDAAYPPPDWAMRPALAPVPETPEAPKAPAVHTGRSAGAKETTYADASN
                     QALLEEMERDESVILMGQDIGPFEGAFRTFKGLHARWPDRVLDTPIAESGTIGIAIGA
                     AVLGFRPVIEMQFGDFISCGFNQLVNVAAKLYYRWQVPCPIVIKLPTGGGVGAGPFHS
                     QNPEGWFAHTAGLKVVCPATAADARSLLKAAVRDPNPVIVCEHKFLYRRIKEVLPEGD
                     DVAVLGEARVLRPGRHLTLVGYGASTWTAMELAGELAKGGVEAEVIDLRTLVPYDEAT
                     VLASVKKTGRALVVHEAQLTLGFGAEVAARLADAAFPWLDAPVRRVTYPDRPVPYSRV
                     LENELLPGKDKVLAAARELLAF"
     gene            complement(6429..8786)
                     /locus_tag="VEL74_09410"
     CDS             complement(6429..8786)
                     /locus_tag="VEL74_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYG62785.1"
                     /translation="MNVREPGTVLDGKYEIVQRLASGGMGEVYQVRHLHLHELRVVKI
                     LRQDLAADSSAQKRFMREARLATQIKHPNVAILYDFSTLPDGSFYMVWEHIEGRDIGD
                     RIRQQGPFPLPMAIQLGIQALRGLEAVHHAGIVHRDVSPDNLMITHDRRGQPLLKIID
                     LGLAKTLEDSTANRDFEITQVGMFMGKLRYCSPEQARAVDGEALDLRTDIYSFGQVLY
                     EMICALPPFDSESQHGFIFKRLSEDPLPLTGRNTNVQVPKELDRVVLKALERERDNRY
                     PDAAVFIQELDAVARGLSAAETQEIPIPPMVRPAAPKPRSSSELSRDERAELLAQIER
                     AGRRTQESAPDVSRAEAALAAGRLDEARDLIGKMEVSHPRAVGTARLKERLSQALEAQ
                     AAARAAEQAAQAAAQAAQAAQAAADRERRTAEAEQVLDGYLQKKQLPLARMALETLLE
                     LQPGHPRRAELEERVEALARELAPPPPAPVPAPAPVQSPQPPQEAQRVAENQEAPSPE
                     RRVAEAEQVLTNYLQKKQLPLARLALETLLELQPKHPRRSELQGRVDALAQELERQKR
                     LTTAQTAGREAIARRDFKAARRELDALVRSDPTGERAAAFHLEMEAAEKEARQESELE
                     EGRRLFEAALGAGKLEEAQRQLDQLASLDLPRVTLDFYRERLDEVRTRTQQEGQIAVF
                     DRRYKAAVKAHDWLAAREVARELAASLPGTPRAAAMFGEVERLEAEHRRQQSIEQGVK
                     QVEAFLGQKDLPKAELAFKILLQMDPENHHRKRFEREIRALQAAK"
     gene            complement(8888..9406)
                     /locus_tag="VEL74_09415"
     CDS             complement(8888..9406)
                     /locus_tag="VEL74_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62786.1"
                     /translation="MRSFTHGSSASEQPNAFTASFLDRMAERDEGPAAPEADVAGPWR
                     IEEVPGEGFGVFRLGESPARGFAPTVAFPSAWLALVAAGVLPGTGREPMLLLRKDRNP
                     DGTYSITLESGEVVGRSHLFDEKLVDSMNMAIHLLRAPESLANVLDAAGLSPLERCGA
                     ILDERVAARAGE"
     gene            10173..10544
                     /locus_tag="VEL74_09420"
     CDS             10173..10544
                     /locus_tag="VEL74_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62787.1"
                     /translation="MASLSFAEELNLNTLLTNNLRSHLGEMPHLTGLHNELEGLVSQG
                     RLLEDQRGFYEGQLRHTNAQRRELEKKGRELRQRLASALRSSFGLDNQKLLEFGVKPR
                     AVRRRRPKPDEPVLEDRADAQ"
     gene            complement(10744..11196)
                     /locus_tag="VEL74_09425"
     CDS             complement(10744..11196)
                     /locus_tag="VEL74_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HYG62788.1"
                     /translation="MTRGTISLKVVHDRLGLVESGLRDLRALPVDSFETFLEDFRNAA
                     AAESLLRRTIEALLDTARHLLAKQFGEGTLEYKKVAQLAVEHGLVLDPETGKRFVLIA
                     GYRNRLTHFYDQVTPEELYGILREELGDLESIVAELRAAAGRISGMGR"
     gene            complement(11193..11624)
                     /locus_tag="VEL74_09430"
     CDS             complement(11193..11624)
                     /locus_tag="VEL74_09430"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYG62789.1"
                     /translation="MDGGAKREALDRICRDRGLLAVYLFGSRADDGLRLLAGEAVDPA
                     GSDLDVGVAFRKPDADASFRVLAQLQIEFEDLFEPLRVDLVPLHRVDPIFQFNAIDGH
                     RIAAPDSDAADFYELRVMREASELLPIQRQLEKDFFGASTT"
     gene            11672..12031
                     /locus_tag="VEL74_09435"
     CDS             11672..12031
                     /locus_tag="VEL74_09435"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HYG62790.1"
                     /translation="MSERELWDLERQFWTGGAPIYEERLAPESLMVFPQPAGVLDRAA
                     TIAAIQQAPRWEDVRFSSQRIAFPGPQVAVLVYEATADRSAPDTEYAAQCSSTYVKLG
                     GSWKLALHQQTPAARRA"
     gene            complement(12115..14037)
                     /locus_tag="VEL74_09440"
     CDS             complement(12115..14037)
                     /locus_tag="VEL74_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62791.1"
                     /translation="MSEDWRAKAVPQSVEVKPPEITAPPAEALSEEKTRGLEDDFKGI
                     REARKRPAKPSRPSKIAVLALHGMGQQIQFETLDQVQRNFCAAAERQGVKLPKPGVQV
                     ARLNGEVMRRAEVRLGEEKGAPEVHFYESYWAPLTEGRVGLRDVISFLRSGGWNGLQH
                     CIRPSSSRYMFGEEVPLPRSGRYAVYLTLALLAVSALVMINTIVLAVAAATNWLGGGS
                     SGWLTPLLLEDLTGVASAVCLVGAVLLSGLLLSTMRRELEGRPWRLPADRRFILDSLF
                     YLTLITLTGVAGALILLVKSHSSSRPLESLLFIVAGGKERAMQIATVLTSAILLSLLT
                     VVAGMLVKAALGKRLPSLARLVVGLAFYLPVACLAGAGGFLIAILLGANVSFFSVSEL
                     FWPLDWLLANRNLWVWSVLIALSAFARSFLVQYLGDVAAYVSPHKLDRFNEIRDQIKK
                     EVLKTARAVYSRSADGSPEYDRIAVVGHSLGSVVAYDTLNCLINEDLLSGSTSDVADR
                     TCLFLTFGSPLDKIAYLFQIQGSRTSDTREALANAVQPLIRNYDHRTFPWINVFSKDD
                     IICGSLELFDDGKGHPRSVHNKEDRYSSIPLVAHTEYWRTPLVWDELFRIVDSEWKSG
                     AQAASQPVEEQQREAG"
     gene            14571..14885
                     /locus_tag="VEL74_09445"
     CDS             14571..14885
                     /locus_tag="VEL74_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62792.1"
                     /translation="MKKLAVATFVFIALVSVFAPAVASACTSPCENICWRPNSCYWSC
                     RYYTGECITCSQFGCGFGFSLQEEPTPQVSTPAQASPKATASDLRQAFWNDLTTAAPA
                     AR"
     gene            complement(14944..15657)
                     /locus_tag="VEL74_09450"
     CDS             complement(14944..15657)
                     /locus_tag="VEL74_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HYG62793.1"
                     /translation="MTLRDLLTDPAYGEMALRLSTSLLIGALIGLERTYHSRPAGFRT
                     HALVCLSTALLMLLTAFGTHWFTGMEGGRVNLDPTRMAQGIMTGIGFLGAGAIIKDGM
                     SVRGLTTAASLWITASIGILIGAGLFFPAILATAFTLGLLALFKRVENRIPVHFVADF
                     FVRFALDGALLEAELRRMMREHGVNAGACSQRLDRDAGFVEYRMSIQSRRDENVRSFA
                     RALGQHPAVRETGYTPAGD"
     gene            15757..16671
                     /locus_tag="VEL74_09455"
     CDS             15757..16671
                     /locus_tag="VEL74_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HYG62794.1"
                     /translation="MAVTMWLRWSACTVVLALGLCAARAQTMTVPAAPPWQEEWLSRF
                     NQERARIGAPPLRLLPVLTRVAQQQAEEMAARRLRSPSTATVSERLRRAGYSARDWRL
                     DYAVATLSPQTGRVDWSWSPGPRAALGKHLRNVGIGAASINGGTFYVFLFGLHEGDYF
                     AGVTGRLDDRARIAAEMLSRVNDIRRQSGLPALALNPLLDRLSQAQAEDMLVRSYYAH
                     QSPEGLGPSDRARAAGYRAGIGENIAEQRFSVDDVLKAWLDSPGHRRNILDPGCREMG
                     LGLAVGAGYDAAPGGYRVVWVQSFGRGE"
     gene            16701..17969
                     /locus_tag="VEL74_09460"
     CDS             16701..17969
                     /locus_tag="VEL74_09460"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG62795.1"
                     /translation="MGQTLRRRQAGADPFGTVQSLEHMAALKKEQSDYAAARAILQET
                     LAAKRRLLGNGDPEVAKTLNQLGETVALAGSLRQAEALHQEALSIARQAEGPQGVTVA
                     ESLLALASRAERRGDYPQAETYHRQGFAILSRAWGEDDPRLAEEQAVLAEVLIDQGKL
                     REAEAFLTPALAAQRKALGDEHPHVSRLFELLATIRGQLGDYRGAEALYRQALAGLLR
                     HYGEQTKRTADVIASLGASLKHQGRYEEAAALLERSLAIRRRILGDRHEEVGHSLLHL
                     AECRRKLGQPEAALTLARQSLSTLETTLGREHPYVAYPLQAIALVLRDQGRTAAAERH
                     FRRALELLSRSVPAGHPQIASAKVELVGCLITLERPDEALRLLRDAEPILAATFGPGS
                     PKGAQGPGAARNARARRQRLKGGRELRPPR"
     gene            complement(17973..18722)
                     /locus_tag="VEL74_09465"
     CDS             complement(17973..18722)
                     /locus_tag="VEL74_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HYG62796.1"
                     /translation="MTILLSFAHPDDESFFTAGISRQHAEAGRSVVLCCATRGERGSA
                     GNPPLTSIEELPATRERELRAAAEIMGIGRIEMLPYRDRELADAPPDEIRASLVSLIR
                     RHRPEVVITFDPQGGNLHPDHIAISRFTTDAVTAAADARFHPESGPPHRVPRLLWVGT
                     VLPWEETDPARLAERPGVDFLIDIRPWRDIKARALAAHRTQHQSIGRYFLERPDRDAI
                     LSFETFRLAAGEPVEAPPLDDLFTGLTAESA"
     gene            complement(18788..19663)
                     /locus_tag="VEL74_09470"
     CDS             complement(18788..19663)
                     /locus_tag="VEL74_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HYG62797.1"
                     /translation="MFFHDKRMQYRAKPDRSDPLYARKLQEVLGGQFGEMTVAMQYLF
                     QGWNCRGPAKYRDMLLDIGTEELAHVEMLATMICQLLEGAPVEDQEAAVRSNSVVGAI
                     MGGSSPRDMLLAGSMSPQHVIVSGLGAMPVDSVGFPWTSRYMIASGNLLADFRHNLTA
                     ESQGRLQVVRLYEMTEDKGVRDMLSFLIARDTMHQNQWIAAIAELEADGLDTTPVPSS
                     FPQNLEHTEVSYQFWNCSVGTESSEGRWARGATPDGKGEFQYVANPEPMGPRPDPAPP
                     APEVHAMNMELVEVG"
     gene            19814..20491
                     /locus_tag="VEL74_09475"
     CDS             19814..20491
                     /locus_tag="VEL74_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62798.1"
                     /translation="MIDDPEQDETEERGQPEGLDPLDAAPFSADEKLPGSEEIFETYF
                     ASWLAAEDLHRHDEEGMRPDVEDGLTSGLPARELSPLTDKGRTIIDEQIRGMVAAAAE
                     DWQGLLSTAGDPSLEWIDAFDRRFGRAEVADIIADADPADDSSDLVVLACELGAVLGE
                     VLRREAPGLEWLHDSPAWTSALYDPRQGRRFNVFHWGIRKFSEDGVDDPLRSKVLRCV
                     GLVRYGA"
     gene            complement(20537..>20683)
                     /locus_tag="VEL74_09480"
     CDS             complement(20537..>20683)
                     /locus_tag="VEL74_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HYG62799.1"
                     /translation="APPAPAAPAAAGGAAAAPGGTPAFCYNCGAKLPPGAKFCPSCGT
                     KMGE"
BASE COUNT         3187 a         7066 c         7349 g         3081 t
ORIGIN      
        1 ggaccgccgt cgagtccagc ggatgctcgg cgccgcggat gcggaggaag ccggcggaca
       61 gctcgaaggt cttggggccg aagccggcga tggagaggag cgacttgcgg gagcggaacg
      121 ggccgttctt gtcccggtgc tccaccaccg cccgcgccag ccgctccgag aggcccgaca
      181 cccggcggag cagcggcgcc gaggcggtgt tgagctccac gccgacgcgg ttgaccgccc
      241 cctcgaccgc ggtgtccagc tcccgctcca gcgccttctg gtcgacgtcg tgctggtact
      301 ggcccacccc catcgaacgc ggctcgatct tcaccagctc ggcgagcggg tcctgaaggc
      361 gccgggcgat cgagaccgcg ccgcgcagcg agacgtcgag ctgcggcagc tcctcgcgtg
      421 ccacggccga ggccgagtag acggaggcgc cggtctccgg gacgatggcg acgaccacct
      481 cctcgatccc ggcctcgcgc accgccttcc tggcgaacgc ctcggtctcg cggctggcgg
      541 tgccgttgcc caccgccaca gccttgaccg cgtgctggcg gatcagcccc acgacgatgg
      601 ccgccgcccg ggcctcgtcg gcgtgcggcg ggatggcgtg gatgacgtcg gtcgccacca
      661 cccggccggt ggggtcgacc accgccatct tgcagccggt gcggtagccg gggtcgagcc
      721 ccatgaccgc gtactggccg agcggcgcct ggaggagcag cgcctcgagg ttggcccgga
      781 agacggcgat ggcctgggct tcggcgcgct cccgcatctc ggagcgcacc tcgttggtga
      841 tcgacggccg gagcagccgc ttgtagccgt cggcggtggc ggccaccacc tcccggccgc
      901 agggggtgtc gaccggcact ccccaggaca tggcgaggtg gccgacctcc cgctcgtcct
      961 cgatgcggag gtcggagagc aggaaaccct cgcgctcgcc gcgcaggatg gcgagcaggc
     1021 ggtgggaggg gatgtccttg cccgcctcct ggtgatcgaa gtagtcgcga tagacgagcg
     1081 cctccggatc cttctccttg ccgggcatga cggcgacggt gagccgcccc tcgttggcga
     1141 gcacccggcg cagctcggcg cggtgctcgg cgttctcgga caggcgctcg gcgaggatgt
     1201 cgcgcgcccc ggccagggcc gccgcggcgt cctccacccc cttctccgga tcgatgaagg
     1261 ggcgcgccag ctcgtcggcc ggggtggagc ccgtagcagc ggccaggacg gcctcggcca
     1321 gcggctcgag ccccttctcg cgggcgatct gggcgcgggt gcggcgcttc ggcttgtagg
     1381 ggaggtagag gtcctccagc tcctgcttgc cgacggcggc gcggatggcc gcctccagct
     1441 cgccggtgag cttgccctgc tcgcggatgc tgtcgaggat ggcatcgcgg cgctcggcga
     1501 gctccaggaa gtactcgcgc cgcttgaaca cggtctcgag ctgcaggtcc tcgaggcccc
     1561 ccgtggcctc cttgcggtag cgcgccacga aggggacggt cgccccctcc gcaaaaaggt
     1621 cggacgcggc cgccacctgc tgcggccggc accccacctc cgacgcgatg atctcgacga
     1681 aacggctctc caggactgcg ctcttgctca tcgactcctc cagctcctcc aagatgacgg
     1741 acagcgtatc ctccagaaag aggggccgga ccgcaagggg gtagaaacag tacagggaga
     1801 ggcccgacta cttgcggccc caccagtaga agggacgccg cttctggttc atgacggcaa
     1861 ggatacggac ggagtcgggg cggaccgagt agagcaggtc gtaaggaaaa cggcgaagga
     1921 tcttgcggcg gaccgtcccc cggacaatgg gcgcggattc aggatagtcg aggatctgtt
     1981 cgaacgcgtc ctcgatctcg ctgacgaatg ccctcccgag gccgggactt tcggcatcgt
     2041 agtagtccac cgcttcatcg agctcttttt tggcgagagg agccagcgag agagagcgtt
     2101 tcacctgagc ctggccctga tctcgcgcag aacctcctcg gcagggatcg cggtggcccc
     2161 cccctcgtcg agctcccggt cgcggcgctc tgcttcctcg aaccacaggt tctcgatctc
     2221 ggcctcagat agatcctcga ggctgtgcag cagcgtcgtg gcgagcttcg cccgaccgtt
     2281 ggggttcagc ttgagggctt cagcttcgat atccgctagg ttcatggcga tcacctcctc
     2341 gggcaggtca gaattatagc cgcgacttcc cgggaacctt tcccccgctg ccgcgttatg
     2401 agggctggag agcgacgatg atcgacccca tcgaggcggt gcacttggga gacgaggagc
     2461 agcccgacga cgccgagctg atggcgcggg tgcggaccgg cgaccgccat gccttcgcgc
     2521 ggctggtcga ccggcacaag gacgcggtgg tcaactacgt cgcccgcctg accggcagcc
     2581 gcgaccgcgc cgaggatctg gcccaggaga ccttcctccg gctgttccgc gccgcggcgg
     2641 gctacctgga gcagggacac ctccgtgcct acctctaccg catcgccacc aacctggtgc
     2701 gctccgagga gcggcgggag cggcggctgc gcctcctcct tcccttcctg gggccggacc
     2761 ggccgcacgc cgagccggcg gcgccgtccg ggctgctgcg ccaggagatc caccgcaagg
     2821 tggccgcggc ggtggcggag ctgcccctgc gcttccgggt ccccctcgtg ctgcacgaga
     2881 tcgaggggtg gccctacgcc gacatcgccc gggagctggg gtgccgcgag gggacggtca
     2941 agtcgcgcat ccaccgcgga cggcaacaac tgaagcagga gctcgagccc tactggaacg
     3001 gaggtacgcc atggaagatc aaccgctagg ccggctactg cgcgagctgc cccgggaacg
     3061 ggcgggggac cgcttcaccg cccgcgtcct cgaaggattg gacggcggcc gccgccccgc
     3121 gcggcgctcc gggtgggcgc tcgctacggc ggccctcctc gccctcctgg tctcgggcgt
     3181 cctggtggag cgccgggggc ttcggcaggg cgagccggcc cattccgagg ccggggaggc
     3241 gcggcaggcg ctcgaggaga tccgcgacga gcacgagcgc ctagcccgcg agctggagcg
     3301 gctccagtcc ctcagcgagg ggcagggcga cgcgccgggg atcgtctacc tcggcggcaa
     3361 cgaacgcatg gacctggtcc tggacctggg acggatgccg gaggggaccc tccccgcatc
     3421 ctatggctcc gagaccttct gacatcaacg tgaacaggga gatatccacg atgaacagca
     3481 gagcactctt tcgcaagacg gcctgggccc tcgccctcgg cctggtgacc ctcgcgcccg
     3541 ccgtttccgc caatgccggg gcggatcagg gggacgacaa ggagaagaag gtcgagaagc
     3601 ggcgcatcgt catcgtcgac aagaacggcg agcggcaggt gatcgagggc gaagggccca
     3661 gcgtccgccg cggctatctg ggcgtcggcc tcaccgacct cacccccgag ctgcgcgccc
     3721 acttcggcgc cagcgagagc gccggggtca tggtctccag cgtcgagccc ggcagcccgg
     3781 ccgacaaggc gggggtcaag gtcggcgaca tcatcacctc gttcgaccgc gacgccgtga
     3841 agtcgagctg ggacgtccgc tccaaggtgc gcggctacga ggacggccag caggtggcgc
     3901 tcgaggtggt gcgcgacggc cgggcgcagt ccttcaccgc ctccatggtc cagcgggagc
     3961 ggccggagtt cgacgtcacg cccttcttct tcggcaagga cggcaaggtg gacccgaagc
     4021 tcttccactt cgagggcgac ctccggaaca tcgagacgat ccagatcccg agctgggaca
     4081 aggagggcgg ccccggcggc cccggtcccc gcgtccaggt gctccgcagc cgtgaggtcg
     4141 agctcgagaa gcagcttcag aagctcgaga agcggatcgc cgagctggag aagcagctcg
     4201 accggaagaa ctaggcgctg cggctccctt ctcttcggcc ccggggggac gtaccccccg
     4261 gggccgctct ttcttcagcc tcagaaagcc agcagctcgc gggccgcggc gagcactttg
     4321 tccttgccgg ggagcagctc gttctcgagc acccgggagt agggcaccgg gcggtccgga
     4381 taggtgaccc ggcgcaccgg cgcgtcgagc caggggaagg cggcgtccgc caggcgggcc
     4441 gccacctcgg cgccgaagcc cagggtgagc tgcgcctcgt ggaccaccag cgcccggccg
     4501 gtcttcttca ccgacgccag caccgtcgcc tcgtcgtacg gcaccagcgt ccgcaggtcg
     4561 atgacctcgg cctcgacgcc ccccttggcc agctcgccgg ccaactccat cgccgtccag
     4621 gtggaggcgc cgtagccgac cagggtcagg tgccggccgg ggcgcagcac ccgcgcctcg
     4681 ccgagcaccg ccacgtcgtc cccctcgggg agcacctcct tgatccgccg gtagaggaac
     4741 ttgtgctcgc agacgatcac cgggttgggg tcgcggaccg ccgccttgag cagcgaccgg
     4801 gcgtccgcgg ccgtcgccgg gcagaccacc ttgagcccgg cggtgtgggc gaaccacccc
     4861 tccgggttct gggagtggaa cgggccggca ccgacgccgc cgccggtggg gagcttgatc
     4921 acgatcgggc agggcacctg ccaccggtag taaagcttcg ccgccacgtt gacgagctgg
     4981 ttgaagccgc aggagatgaa gtcgccgaac tgcatctcga tcaccggccg gaagccgagg
     5041 accgcggcgc cgatggcgat gccgatcgtg ccgctctcgg cgatcggcgt gtcgagcacc
     5101 cggtcgggcc agcgggcgtg gagccccttg aaggtgcgga acgccccctc gaacgggccg
     5161 atgtcctggc ccatgaggat caccgactcg tcgcgctcca tctcctccag cagcgcctgg
     5221 ttggaggcgt cggcgtaggt ggtctccttg gcccccgcgg accggcctgt gtggaccgcc
     5281 ggcgccttgg gagcctccgg agtctccggg acgggggcga gcgccggccg catcgcccag
     5341 tccggcggcg ggtaggcggc gtcgagggcg gacgagatcg cccgctccac cagctcggcg
     5401 gtgcgggcct cgagccgggc gcgcagcccg gcgtcgagca ccccctcctc ctccagcctc
     5461 ttggcgtagg ccggcaccgg atcggcggcg cggtaggcgg cgagctcgtc ggcggggacg
     5521 accttcagcg agtcgtcccc ttcgctgtgg ccgcgcatgc ggccgagcat cgcctcgatg
     5581 agggtggggc cgtggccggc gcgggcgcgg gcgacggcgc ggtccagcac ctcggccatg
     5641 gcgtccgggt cgttgccgtc caccgtctcg gccgggatgc cgtagcccgg gccgcggtcg
     5701 ctcaggcgct tggcggcgta ctggagatgg gccggggtcg agaaggcgta gcggttgttc
     5761 tcgatgatca ggatcagcgg cagcttccag acggcggcca tgttgagccc ctcgtggaag
     5821 tcgccggtcg aggtggcgcc gtcgccgatg aagttgatgg cgacgcggtc ggtgccgcgc
     5881 tgccggaacg cgaagccgca gccggcggcg accgggatca tcgagccgag gtggctgatc
     5941 atgcccaggt ggaggatgcc gttctccggc gccaggtagc cgtagtggaa cgagcgctcg
     6001 aacccctggg agaagccgtc ggccttgccg aggagctggc aggccagccg gtagaggatc
     6061 cgctccgggt cggggcgccg gccgtcgggg tcgccgaagc cgaggccggg gaaggcgcgc
     6121 gccggatcga gatagaccgg caggatcgcc ccgaggtcgc ggtgcagcgt gcagagcgcg
     6181 tcccccgact ccaaggccaa gccggcgggg atggtggtgg cctcgttgcc gacctcgctg
     6241 taccacttgg acacgcggcc ggtgagcagc agggcctcga agcgggagtc gaggtcccgc
     6301 gtcagcttca tgaaggcata tcgcagcagg cgctcccgcg gagtatggaa cgggttgcgg
     6361 aacgcccgcg gctggagaag ggctgtcgtc gtcataaccg gcgcagtgta tcaggggccg
     6421 tgaacaggtt atttcgcggc ttgcagggcg cggatctcgc gctcgaagcg cttgcgatgg
     6481 tggttctcgg ggtccatctg gagcaggatc ttgaacgcca gctcggcctt gggcaggtcc
     6541 ttctggccga ggaaggcctc gacctgcttg accccctgct cgatcgactg ctgccgccgg
     6601 tgctcggcct cgagccgctc cacctcgccg aacatggcgg ccgcgcgcgg cgtccccggc
     6661 agcgaggccg cgagctcgcg cgccacctcg cgggcggcga gccagtcgtg cgccttgacc
     6721 gcggccttgt agcggcggtc gaagacggcg atctgcccct cctgctgggt gcgggtgcgc
     6781 acctcgtcca ggcgctcgcg gtagaagtcg agggtgaccc gcggcaggtc gagggaggcg
     6841 agctggtcga gctggcgctg ggcctcctcc agcttgccgg cgccgagcgc ggcctcgaac
     6901 aggcggcgcc cctcctccag ctccgactcc tggcgcgcct ccttctcggc ggcctccatc
     6961 tccagatgga aagccgcggc ccgctcgccg gtcggatcgc tgcgcaccag ggcgtccagc
     7021 tcgcgccggg cggccttgaa gtcgcgccgg gcgatcgcct cgcggccggc ggtctgtgcc
     7081 gtggtcaggc gcttctgccg ctccagctcc tgggccaggg cgtcgacgcg cccctgcagc
     7141 tcggagcggc gggggtgctt cggctgcagc tccagcagcg tctccagcgc caggcgggcg
     7201 agggggagct gcttcttctg cagatagttg gtgagcacct gctcggcttc ggcgacccgg
     7261 cgctccgggg agggcgcttc ctggttctcg gccacgcgct gcgcctcctg cgggggctgg
     7321 ggggactgga cgggagccgg cgcgggcaca ggcgcgggag gcggcggcgc gagctcgcgg
     7381 gcgagcgcct cgacccgctc ctccagctcg gcccggcggg ggtgcccggg ctgcagctcc
     7441 agcagcgtct ccagggccat gcgggcgagg gggagctgct tcttctgcag atacccgtcg
     7501 agcacctgct ccgcttcggc ggtgcggcgc tcgcggtcgg ccgcggcttg cgcggcctgg
     7561 gccgcctggg ccgccgcctg cgcggcctgc tccgccgccc gcgccgccgc ctgggcttcg
     7621 agggcctggc tcagccgctc cttgagccgg gcggtcccga cggcccgggg gtgggacacc
     7681 tccatcttgc cgatgaggtc ccgcgcctcg tcgaggcgtc cggcggcgag ggcggcctcg
     7741 gcgcgggaga cgtcgggggc gctctcctgg gtccgccggc cggcgcgctc gatctgggcg
     7801 agcagctccg cccgctcgtc gcgcgacagc tcgctcgagc tgcgcggctt cggtgcggcg
     7861 ggacggacca tgggcgggat ggggatctcc tgcgtctcgg cggcgctcag gccgcgggcc
     7921 accgcgtcca gctcctggat gaagacggcg gcgtcggggt agcggttgtc gcgctcgcgc
     7981 tccagcgcct tgaggaccac gcggtcgagc tccttgggga cctggacgtt ggtgttgcgg
     8041 ccggtgagcg gcagcgggtc ctcggacagg cgcttgaaga tgaagccgtg ctggctctcg
     8101 gagtcgaacg gcggcagggc gcagatcatc tcgtagagca cctggccgaa cgagtagatg
     8161 tcggtgcgca ggtcgagcgc ctcgccgtcg accgcccgcg cctgctcggg ggagcagtag
     8221 cggagcttgc ccatgaacat ccccacctgg gtgatctcga agtcgcggtt ggcggtcgag
     8281 tcctccagcg tcttggcgag gccgaggtcg atgatcttga gcagcggctg gccgcgccgg
     8341 tcgtgggtga tcatcaggtt gtccggcgac acgtcgcggt ggacgatgcc cgcgtggtgg
     8401 accgcctcga ggccgcgcag ggcctggatg ccgagctgga tggccatggg caacgggaac
     8461 ggcccctgct ggcggatgcg gtcgccgatg tcgcgcccct cgatgtgctc ccagaccatg
     8521 tagaagctgc cgtccggcag ggtcgagaag tcgtagagga tggcgacgtt ggggtgcttg
     8581 atctgggtgg cgaggcgggc ctcgcgcatg aagcgcttct gcgccgacga atcggcggcg
     8641 aggtcctggc gcaggatctt gaccacccgc agctcgtgca ggtggaggtg gcgcacctga
     8701 tacacctcgc ccatcccgcc gctggccagg cgctgcacga tctcgtactt gccgtcgagg
     8761 acagtccccg gttcacgaac gttcaaggcg gttcccctcg gagtgagggt ccattgtacc
     8821 gtgaagggga ggtggggggc ggccccccgc gaccacgaga gccgcccacc tatctaaaag
     8881 gaagggccta ctcccccgcc cgggccgcca cccgctcatc caggatggcg ccgcagcgct
     8941 ccagggggga gaggccggcg gcgtccagga cgttcgccag cgactccggc gcccgcagca
     9001 ggtggatggc catgttcatg gagtccacca gcttctcgtc gaagagatgg cttcggccga
     9061 cgacctcgcc gctctccagg gtgatggagt aggtgccgtc ggggttgcgg tccttccgga
     9121 ggaggagcat cggctcgcgg ccggtgccgg ggaggacgcc ggcggcgacg agtgcgagcc
     9181 atgcggacgg gaaggccacc gtgggcgcga agccgcgggc cgggctctcg ccgagccgga
     9241 acaccccgaa gccctccccc gggacctcct cgatccgcca cggaccggcg acgtccgcct
     9301 ccggcgcggc gggaccctcg tcccgctcgg ccatgcggtc caggaacgaa gcggtgaagg
     9361 cgttgggctg ctcggacgca ctgctaccat gagtgaagct acgcataaag ggttacctcc
     9421 ctttttgtgt ggcgaggctc gggagggagg tctgaactcc cacccgggcc gccacacggt
     9481 gtctgtgaac cgcggccggc accacgccgg accacgggtg ctgcgtactt cctgatacaa
     9541 gaatcctaga acgaaggtgg ggcagaagtc aacgcccctt cgcgggactt catacccccg
     9601 gtaccgacag cggtatcccc tcggcaaaaa accaaggtac atccgaacgc ggcgccgccc
     9661 gacccaggcg ccgcgctccg gatatgcacg tcaattgtca aagaacctgg cgccggccct
     9721 gcggcttcgc gccatcaaca tcaatgatgc cacagatccc cccggcaaga cactgttgac
     9781 aactagatct catgcgttgt agattctcgc cgctggattc aacgcgagag gagaaagatc
     9841 taccgatgag tcggctgtcc aaccaaaacg gatgctgtcc agggaagctg gatgccgtcc
     9901 ggtccgttcg ggaagcgatc cggggcgtca ggacggctac tggagcggcg ggtatcgatc
     9961 cgccgcgtcc gggaagccgc ccgagccgtc cggatggaat ccgcggcgcc gggaaggcat
    10021 ccggcgggtc cgggaagcgt ccgagccgtc cgggaagcgc ccggggaccg ccccggggcc
    10081 gttcgaggag atctggcgtc cccatccggg ccgggagcgc gtcgctgccg ggcccgtaca
    10141 agccgaacca tgcttataaa aggagaagga ccatggcgag tctgtcgttt gcggaagagc
    10201 tgaacctgaa caccctgctg accaacaacc tgcgttccca tctcggggag atgccgcacc
    10261 tgaccgggct ccacaacgag ctcgagggcc tggtttccca ggggaggctc ctggaagacc
    10321 agcggggctt ctacgagggc cagctgcggc acaccaacgc ccagcggcgg gagctggaga
    10381 agaaggggcg cgagctgcgg cagcgcctgg cctcggcgct gcgcagcagc ttcgggctgg
    10441 acaaccagaa gctgctggag ttcggcgtca agccgcgcgc cgtccgccgc cgccggccga
    10501 agcccgacga gccggtgctc gaagaccgtg ccgatgctca gtaatcagta ggaagcaagc
    10561 ccggcgctcc agtccttgga ccggagcgcc gggtgccccg ttctttgaca actgaattgt
    10621 gacgggggcg ccgccgtgtc caaggccgcg cccccccgag ggctcctccg tccgacagga
    10681 ggaaacccgc ccggcggagg tccgatccct ccggcgggcg ggggatttgc cgccggcttc
    10741 agatcaccgc cccatccctg aaatccgccc ggccgccgcc cgcagctccg cgacgatgct
    10801 ctcgagatcc cccagctcct cccgcaggat tccgtacagc tcctcgggcg tgacctggtc
    10861 gtagaagtga gtcagccggt tgcggtagcc ggcgatgagc acgaacctct ttccagtttc
    10921 gggatcgaga accaggccgt gctcgaccgc gagctgcgcc accttcttgt actccagcgt
    10981 gccctctccg aactgcttcg ccagcagatg gcgcgctgtg tccaggagcg cctcgatggt
    11041 tcgccggagc aaggactccg cggccgcggc gttgcggaaa tcctccagga atgtttcaaa
    11101 ggaatccacc ggcagagcgc gcaggtcgcg caatccactc tcgacaaggc cgagacggtc
    11161 gtggacgacc ttgaggctga tggtcccccg ggtcacgtcg tgctggctcc gaagaaatcc
    11221 ttttccaact gccgctggat cggcagcagc tccgaggcct cccgcatcac ccgcagctca
    11281 tagaagtcgg ccgcgtcgga gtcgggggcg gcgatgcggt gaccgtcgat ggcgttgaac
    11341 tggaagatgg ggtccacgcg gtgcagaggg acgagatcga cccggagcgg ctcgaagagg
    11401 tcctcgaatt cgatctggag ctgggcgagg acgcggaacg acgcgtcggc atcgggcttc
    11461 cggaaggcga ctcccacatc gagatccgag cccgccgggt ccaccgcctc gccggcgagc
    11521 agccgcagcc cgtcgtcggc gcgcgagccg aagagataga ccgccaggag gccgcggtcg
    11581 cggcagatcc ggtccagagc ctcgcgcttc gcccctccgt ccatggcgcc gctgattatg
    11641 gcatgctccc caagagcaga aggagcagac gatgtccgaa cgagagctat gggacctcga
    11701 acgacagttc tggaccggcg gcgcgccgat ctatgaagag cgcctggctc cggaatctct
    11761 catggtgttc ccccagcccg cgggcgttct ggaccgggcc gccacgatcg ccgcgattca
    11821 gcaagctccg cgctgggagg acgtccgttt cagctcgcaa cggatcgcct ttccgggccc
    11881 acaggtcgcg gtcctggtct acgaagcgac agccgaccgc agcgcgccgg atacggagta
    11941 cgcggcgcaa tgcagctcga cctacgtaaa gctcggcggg agctggaagc tcgctctcca
    12001 ccagcagact cctgcggccc gccgagcgta ggccgatcgg cctgcgcctg cgtctcgagc
    12061 ccgccacggc cgaagaggcg ccaccggccg agaccaaggc cggcggcgac ctcctcaacc
    12121 cgcctcgcgc tgttgctcct ctaccggctg agaagcggcc tgagccccac tcttccactc
    12181 ggagtccacg atacgaaaga gctcgtccca gaccagggga gtccgccaat actcggtgtg
    12241 ggcgaccagg gggatgctgg aatagcggtc ctccttattg tgaacgctcc gcggatggcc
    12301 cttcccgtcg tcgaagagct cgagcgagcc gcagatgatg tcatccttcg agaagacgtt
    12361 gatccatggg aacgttcgat ggtcgtagtt cctgatcagc ggctggacag cattggccag
    12421 agcctcgcga gtgtccgagg tgcggctccc ctggatctgg aacaggtagg cgatcttgtc
    12481 gaggggggag ccgaaggtca ggaacaggca ggtcctgtcg gcgacatcgg aggtcgagcc
    12541 cgacagcagg tcctcgttga tgaggcagtt gagcgtatcg taggccacca cagagcccag
    12601 ggaatgcccc accactgcga tccggtcgta ctcgggactc ccgtccgccg agcgcgaata
    12661 gacggcgcgg gcggtcttca acacctcttt cttgatctga tcccggatct cattgaaccg
    12721 gtccagcttg tggggcgaca catacgccgc cacgtctccc agatattgga caaggaagct
    12781 ccgcgcgaag gcgctgagcg cgatcagcac cgaccagacc cagagattcc ggttggcgag
    12841 gagccagtcc agaggccaga agagctccga caccgagaag aaagacacgt ttgcgccgag
    12901 caagatcgcg atcaggaatc ctccggcgcc tgccagacag gccacgggca gatagaaagc
    12961 gagccccacg accaggcggg ccagggacgg cagcctcttg ccgagagccg ctttgaccag
    13021 catgccggcc acgacggtca ggagggaaag gagtatcgca gaggtcagta ccgtggcgat
    13081 ctgcatcgcc cgctctttgc cgccggccac gatgaagaga aggctttcta acggccggct
    13141 ggaggaatgg ctcttgacga gcaggatgag ggcccctgcc acaccagtga gagtgatcag
    13201 ggtcagatag aacagagaat cgaggatgaa gcgtcgatcg gccggcagcc gccagggccg
    13261 gccttccagt tcacgcctca tcgtactgag caagaggccc gacaagagga ccgccccgac
    13321 gaggcagacg gcgctcgcca ccccggtcag gtcttccaga aggagcgggg tcagccagcc
    13381 ggacgagccg cctcccagcc agttcgtcgc cgcggccacg gccagcacga tcgtgttgat
    13441 catgacgagg gcgctcaccg cgagcagggc cagcgtcagg tagacggcgt atcggcccga
    13501 gcgtggcaag gggacctcct caccgaacat gtatcgcgag gaagaggggc ggatgcagtg
    13561 ctggagtccg ttccatccgc cgctgcgcag gaaggagatg acgtcgcgca gtccgacccg
    13621 tccctccgtc aagggggccc agtagctctc atagaagtgc acctccgggg cccctttctc
    13681 ctcccccaat cggacttcgg cacgccgcat gacttcgccg ttgagccgcg ccacctggac
    13741 ccccggcttg ggcagcttga caccctgccg ctccgctgcg gcgcaaaagt tgcgctgcac
    13801 ctgatccaag gtttcgaact ggatctgctg ccccattcca tgcagggcaa ggaccgcgat
    13861 cttggagggc ctggacggct tcgcgggccg cttcctggcc tcgcgtatcc ccttgaaatc
    13921 gtcttccagc ccccgggtct tctcctctga cagagcttcg gccggcgggg cggtgatctc
    13981 agggggcttg acctcgacac tctgcgggac ggccttggca cgccagtctt cggacatcca
    14041 tacctccttg agggatgctt ctctagagaa gttgccgcag gggtgcctgg aatggacact
    14101 ctctcggcct catcccctga tggacggaca cccgcgctcc ttttttcttc ctccctttcc
    14161 tgcgaacgcc atatagccat cggcacactt gagaagaacc agccggtggt gcggccggtt
    14221 cacgtctcag ctcggaaaga aatcggccct tttctttcaa ggcctcgttg acttggaaag
    14281 ccggctttca aagctcgggg agcttggaaa gaaatcgggc caattctttc cgagttggac
    14341 gcgcttggcg agtgggcgcg tgccgcgaag accgaaagcc cgggccgcca tgatcgtagc
    14401 ggcgctgatg atcgtgtccg ctcttcccgg agggagccag tacgcaccgg cgcccgccgg
    14461 tcggcccagc ccgtcgagcc ctaggggcga ggctcggccc cggccggctc acgcaccttg
    14521 aatgtattgc gaagcgattc attcaggacg cttcattcag ggagacaagc atgaagaagc
    14581 ttgcggtcgc gacttttgtg ttcatcgcct tggtcagtgt gttcgcgccg gccgtcgcct
    14641 cggcctgtac cagcccctgc gagaacatct gctggcggcc gaactcctgc tactggtcct
    14701 gccgctacta cacgggcgag tgcatcacct gctcgcagtt cgggtgcggc ttcggcttca
    14761 gcctgcagga ggagcccacg ccgcaggtct ccacccccgc ccaggcctcg cccaaggcca
    14821 cggccagcga ccttcgccag gcgttctgga acgacctcac caccgccgcg cccgcggcgc
    14881 gctgagatcc tcgccgcccg atggggagcc ggacgttcct cctagggacc caggctcccg
    14941 gcctcagtcg cccgccggcg tgtagccggt ctcccggacc gcggggtgct gtccgagggc
    15001 gcgggcgaag ctcctgacgt tctcgtcccg gcggctctgg atcgacatcc ggtactcgac
    15061 gaagccggcg tcgcggtcga gccgctggct gcaggcgccg gcgttgaccc cgtgctcccg
    15121 catcatccgg cggagctcgg cctcgaggag ggcgccgtcg agcgcgaacc gcacgaagaa
    15181 gtcggccacg aagtgcacgg ggatccggtt ctcgacccgc ttgaacagcg ccagcaggcc
    15241 aagcgtgaac gccgtcgcga ggatcgcggg gaagaagagc ccggcgccga tcaggatgcc
    15301 gatcgaggcg gtgatccaca gcgaggccgc ggtggtgagg ccgcgcaccg acatcccgtc
    15361 cttgatgatc gcgccggccc cgaggaagcc gatgccggtc atgatcccct gtgccatccg
    15421 cgtcgggtcg aggttgacgc gccccccttc catgcccgtg aaccagtggg tgccgaacgc
    15481 cgtcagcagc atcagcagcg ccgtggacag gcagaccagg gcatgggtgc ggaagccggc
    15541 gggccggctg tggtaggtcc gctccagccc gatgagcgcg ccgatgagga gggaggtgga
    15601 cagccgtagc gccatctcgc cataggccgg gtcggtgagg aggtcgcgca acgtcatggg
    15661 cgcattctag tccgcggcaa gccatgcgcg tcctgctata ccttcaggtc cctcttggag
    15721 tatcgtagag ccacctgttc ctggaggcga agtgtcgtgg ccgtaacgat gtggctccgc
    15781 tggtctgcct gtaccgtggt cctcgcgctc ggcctctgcg ctgcgcgcgc ccagacgatg
    15841 accgtaccgg ccgcgccccc ctggcaggag gagtggctga gccgtttcaa ccaggagcgg
    15901 gcgcggatcg gcgcgccgcc gctacgtctg ctgccggtgc tgacccgggt ggcccagcag
    15961 caggcggagg agatggccgc gcggcggctc cgctccccct ccaccgcgac ggtctccgag
    16021 cggctgcggc gggcggggta ctcggcgcgc gactggcggc tggactacgc cgtggccacc
    16081 ctctctcccc agaccgggcg cgtcgactgg agctggagcc cgggcccccg ggcggcgctg
    16141 gggaagcacc tccgcaacgt gggcatcggc gccgcctcca tcaacggcgg cacgttctac
    16201 gtcttcctct tcggcctgca cgagggggac tacttcgccg gcgtcacggg ccggctcgac
    16261 gaccgggcgc ggatcgcggc cgagatgctg tcgcgggtca atgacatccg ccggcagtcc
    16321 gggctgccgg cgctcgcgct caaccccctc ctcgaccggc tgtcgcaggc gcaggccgag
    16381 gacatgctgg tgcgctccta ctacgcgcac cagagccccg aaggcctggg tccgtccgac
    16441 cgcgcccggg cggcggggta ccgcgccggc atcggcgaga acatcgccga gcagcgcttc
    16501 tccgtcgacg atgtcttgaa agcctggctg gacagcccgg gccaccgccg gaacatcctc
    16561 gacccgggct gccgcgagat gggcctgggg ctcgccgtcg gtgccggcta cgacgcggcg
    16621 ccgggcggct accgcgtcgt ctgggtgcag agcttcgggc gcggggagtg aggcggccgg
    16681 ggccgccccg ttgacgtcag ttggggcaga ccctacgccg acggcaagct ggagccgatc
    16741 cgttcgggac ggtccagagc ctcgagcaca tggccgcgct gaagaaggag cagtccgatt
    16801 acgcggcggc gcgggccatc ctgcaggaaa ccctggccgc caagcgccgc ctgctgggga
    16861 acggcgatcc ggaggtcgcc aagaccctca atcaactggg ggaaaccgtc gccctggcgg
    16921 gatctctccg gcaggccgaa gccctccacc aggaggcgct ctcgatcgcc cggcaggccg
    16981 agggtccgca gggggtgacg gtggcggagt ccctgctggc gctggcgagc cgagcggaac
    17041 gcaggggtga ctacccgcag gccgagacgt atcaccggca gggcttcgcg attctgagcc
    17101 gcgcgtgggg cgaggacgat cccagactcg ccgaggagca ggccgtcctg gcggaggtcc
    17161 tgatcgacca ggggaagctt cgcgaggccg aggcattcct gacgcctgcc ctcgcggcac
    17221 agcggaaggc gctgggggac gaacatcctc acgtgtcccg gcttttcgag ctgctcgcga
    17281 ccatacgggg gcagctaggg gactaccgtg gtgccgaggc cctctaccgg caggcactgg
    17341 cagggctgct gcgccattac ggagaacaga ccaaacggac cgccgacgtg atcgccagcc
    17401 tcggagcatc cctcaagcac cagggacggt acgaagaggc ggccgctctg ctggagcgga
    17461 gcctggcgat ccgccggcgg attctcggtg accggcatga agaggtgggg cacagcctgc
    17521 tccatctcgc cgagtgccgg cggaagctcg ggcagcccga ggccgcgctc accctggcgc
    17581 gacagtcctt gtcgacgctg gagacgacgc tgggccggga acatccgtac gtcgcctatc
    17641 cgctccaagc cattgccctc gtcctgcgcg accagggtcg gacggcggcg gcggagcgcc
    17701 atttccgccg ggcgctcgag ctcctcagca ggtccgtgcc ggcgggccat ccgcagatcg
    17761 cgtccgccaa ggtggagctc gtggggtgcc tgatcacgct ggaacggccg gacgaggcct
    17821 tgcgcctgct gcgcgacgcg gagcccatcc tcgccgccac cttcggcccc ggctccccga
    17881 aaggtgcgca aggtccggga gcagctcgca acgctcgagc gcgccggcag cggctcaagg
    17941 ggggccggga gctccggccc cctcggtaga gattaggcgc tttccgcggt caggccggtg
    18001 aagaggtcgt ccagcggcgg tgcttccacc ggctctcccg ccgcgagcct gaaggtctcg
    18061 aagctcagga tggcgtcgcg gtccggccgc tccaggaagt accggccgat ggactggtgc
    18121 tgcgtgcggt gggcggcgag ggcccgcgcc ttgatgtcgc gccagggccg gatatcgatc
    18181 aggaaatcga cgcccggccg ctccgcgagg cgcgccgggt cggtctcctc ccaggggagg
    18241 accgttccca cccagaggag gcgcggcacc cggtgcggcg ggccggactc gggatggaag
    18301 cgggcgtccg cggcggcggt cacggcgtcg gtggtgaaac ggctgatggc gatgtgatcg
    18361 gggtgcaggt tgccgccttg ggggtcgaag gtgatcacga cctccgggcg atggcggcgg
    18421 atcagggata ccagcgaggc gcggatctcg tcggggggtg cgtcggcgag ctcgcggtcc
    18481 cggtaaggca gcatctcgat ccggccgatg cccatgatct ccgccgccgc gcgcagctcc
    18541 cgctcgcgcg tcgcgggcag ctcctcgatg gaggtcaacg gcggattgcc ggcggagccg
    18601 cgctcgcccc gcgtggcaca gcagagcacg acgctcctcc ccgcctccgc gtgctggcgg
    18661 gagatcccgg cggtgaaaaa gctctcgtcg tccggatgcg cgaagctgag gaggatggtc
    18721 atgagagaga tggaaaaggg gccccggatg caccgggacc cccgtggata ctagcaggcg
    18781 caggcgctca gccgacttcc accagctcca tgttcatggc atggacctcc ggcgccggcg
    18841 gggccgggtc gggacgcggc cccatgggct cgggattggc gacgtactgg aactccccct
    18901 tcccgtccgg agtggcgccg cgggcccagc ggccctcgct gctctccgtg cccacggagc
    18961 agttccagaa ctggtaggag acctcggtgt gctcgaggtt ttgcgggaag ctgctcggca
    19021 cgggcgtggt gtccaggccg tcggcttcca gctccgcgat cgcggcgatc cactggttct
    19081 ggtgcatcgt gtcgcgggcg atcaggaagc tcagcatgtc ccgcaccccc ttgtcctcgg
    19141 tcatctcata gaggcgcacc acctgcaggc gcccctgcga ctccgccgtg aggttgtgcc
    19201 ggaagtcggc cagcaggttg ccgctggcga tcatgtagcg cgaggtccag gggaacccga
    19261 cgctgtccac cggcatcgcc ccgagccccg agacgatgac gtgctggggg ctcatggagc
    19321 cggcgaggag catgtcgcgc gggctgctcc cgcccatgat cgcgccaacc accgagttcg
    19381 accgcaccgc cgcctcctgg tcctccaccg gcgccccttc gaggagctgg cagatcatgg
    19441 tggcgagcat ctcgacgtgg gccagttcct cggtgccgat gtccaggagc atgtcccggt
    19501 acttggctgg cccgcgacag ttccacccct ggaagaggta ctgcatggcg accgtcatct
    19561 cgccgaactg gccgcctagg acctcctgga gcttgcgggc gtagagcgga tcggagcggt
    19621 caggcttggc gcggtactgc attcgcttgt cgtggaaaaa catcgtgcct cctttgaagt
    19681 actgcctcgg atgaatgacc ggagacgtta aaccggcggg cacgcgccgc cggagatcta
    19741 ttccatgcaa ttccgatgcg aggtctccgg aaccggccgg accgggcgcg gcgcgtagta
    19801 cgatcgctcc gccatgatcg acgaccccga acaggacgaa accgaagagc gtgggcagcc
    19861 ggagggtctc gatcctctgg acgccgcccc cttctccgcc gacgagaagc tgccggggag
    19921 cgaggagatc ttcgagacct acttcgcctc ctggctcgcc gccgaggacc tgcaccggca
    19981 cgacgaggag gggatgcgcc cggacgtgga ggacgggctg acctccggcc tgccggcgcg
    20041 ggagctgagc ccgctcaccg acaaggggcg gacgatcatc gacgagcaga tccgcggcat
    20101 ggtggcggcc gcggccgagg actggcaggg actgctgagc accgcgggcg atccctcgct
    20161 cgaatggatc gacgccttcg atcgccgctt cggccgcgcc gaggtggccg acatcatcgc
    20221 cgacgccgac ccggcggacg acagctccga cttggtggtc ctagcctgcg agctgggcgc
    20281 cgtgctgggg gaggtgctgc gccgcgaggc ccccgggctc gaatggctcc acgactcgcc
    20341 ggcctggacg tcggccctct acgacccgcg ccaaggccgc cgcttcaacg tcttccactg
    20401 ggggatccgg aaattcagcg aggacggcgt cgacgaccct ctgcgcagca aggtgctgcg
    20461 ctgcgtcggc ctggtgcgct acggagcttg aggaagagcg gaggcggggc gaggccccgc
    20521 ctcccgaagc ggtctactac tcacccatct tcgtgccgca ggagggacag aacttggccc
    20581 ccggcggcag cttggcgccg cagttgtagc agaaggcggg ggtgccgccc ggcgccgcgg
    20641 cggcacctcc ggccgcagca ggagcggcgg gtgccggcgg cgc
//
LOCUS       DASPJI010000356         4077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_254123, whole genome shotgun sequence.
ACCESSION   DASPJI010000356 DASPJI010000000
VERSION     DASPJI010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254123"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VEL74_09485"
     CDS             complement(<1..849)
                     /locus_tag="VEL74_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HYG62800.1"
                     /translation="MTEWDRFLSAQEAAAELRVSLPTLYAYASRGMLRSEAVPGEPRA
                     RRYPREDVLRLKERKEMRREPERVAPKALDWGAPVLESAITLVRGGRLYYRGHDVVEL
                     ARQRTVEEVAALIWAGSFAAAEELFQGDPPELPLLALPELGPVERCQVALPLAGAADL
                     AAWDLRPRAVAITGGRILRLLARVAAGAEKGPAPAGVAEALQQGWAPGRPEASGPIAA
                     ALILAADHELNVSAFTARCVASSQANPYDVVTAGLAALKGRRHGGCTERMEALFREAE
                     AGSGPRG"
     gene            955..2100
                     /locus_tag="VEL74_09490"
     CDS             955..2100
                     /locus_tag="VEL74_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase/methylcitrate synthase"
                     /protein_id="HYG62801.1"
                     /translation="MSSSLETETREGLEGVVAATTRLSSVDGEAGVLLIAGFPVEDLA
                     PVASFEEVVYLLWNGALPRPDELAAFARRMAARRALPGAALDLLRAAAGRRVPVMDAL
                     RMAAGTLSLLPGTGEDAREDGLLLVAAFPTIVASYWRLLNSEEPVEPREDLGHAASFL
                     HLLSGEEPAAERARGVETYLNTVVDHGFNASTFTARVIVSTRSDLVSAVTGAVGALKG
                     PLHGGAPGPALDMVFEIGSAERAEAVLRDKLARGERLMGFGHRVYRVRDPRADVLAEA
                     AERFYKDKGDRGVYELARAVEATALRLLREHKPDRRIDTNVEFYTALLLHGLGLPTEL
                     FTPAFAIGRVAGWVAHGLEQLAHGRLIRPQSAYVGERDRRWVPVEAR"
     gene            complement(2424..>4077)
                     /locus_tag="VEL74_09495"
     CDS             complement(2424..>4077)
                     /locus_tag="VEL74_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG62802.1"
                     /translation="CREKDAALAELEGRLTDQQRIEEERAQLFRILESERSRLARVFM
                     KAPAFIATLRGPDQVFELVNPTFEQLVGFRDILGKPVRQALPELAGQGFFELLDQVYR
                     TGESFTGRERPIHLQREPGGPLDERAVDFVYLPLLDPGEKVSGIFVHGVDVTEPVRAR
                     RRLEELVQELQQAEEALKEADRRKDEFLAMLGHELRNPLAPIRNAVFVMRQLGSGVPE
                     IERGRDMIDRQVSHLARLVDDLLDVSRISRGKILLRQEALDLVALARTAAEDHRSDLE
                     AAGLHLSVRLPEGPVWVQGDPTRLSQVLGNLLHNAGKFTDAGGRVELEMVADPAAAFV
                     GITLRDTGIGMEPRMVERLFQAFSQADHSLDRSRGGLGLGLALVKGLVDLHGGEVHAA
                     SDGLGRGSVFTLRLPRREAAEPAGSRPEAAPAPPGLHRILVIEDNVDAAESMQLLLEL
                     AGHQVTTAFDGPSGVSEAHRFQPSVVLCDIGLPGDMDGYAVARELRRDGLPASAKLIA
                     LTGYGQEEDQRRSREAGFDLHLTKPVDPAALGKLLAELPAGA"
BASE COUNT          557 a         1497 c         1491 g          532 t
ORIGIN      
        1 cccacggggc ccgctccccg cctccgcctc gcggaacagc gcctccatcc gctccgtgca
       61 gccgccgtgg cgccgcccct tgagcgccgc gaggcccgcc gtcaccacgt catagggatt
      121 ggcctgcgac gaggcgacgc agcgcgccgt gaaggccgag acgttgagct cgtggtcggc
      181 cgccaggatc agcgccgccg cgatgggccc ggacgcttcc gggcgtcccg gtgcccaccc
      241 ctgctggagg gcctcggcga cgccggcggg ggcgggcccc ttctccgccc ccgccgccac
      301 ccgcgccagc aggcggagga tgcgcccgcc ggtgattgcc actgcgcgcg gccgcaggtc
      361 ccaggccgcg aggtcggcgg cgccggcgag cggcagcgcc acctggcagc gctcgaccgg
      421 ccccagctcg ggtagcgcga gcaggggcag ctccggtggg tcaccctgga acagctcctc
      481 cgccgcggcg aacgagccgg cccagatcag cgccgccacc tcctcgacgg tccgctgccg
      541 cgccagctcc acgacatcgt ggccgcggta gtagaggcgc ccgccgcgca ccagcgtgat
      601 cgccgactcg agcaccggcg ccccccagtc cagcgccttc ggcgccaccc gctccggctc
      661 gcgccgcatc tccttgcgct ccttgaggcg cagcacgtcc tcgcgcgggt agcgccgcgc
      721 ccgcggctcc cccggcaccg cctcggaccg cagcatgccg cggctcgcgt aggcatagag
      781 cgtcggcagc gacaccctca gctccgccgc ggcctcctgc gcgctcagga aacgatccca
      841 ctccgtcacc ggcacctcct ccccctccaa gctctcatac attgattggc gaatcaagat
      901 tgacaatctt ttgggccgga gtgaatctgg tggggagatg gaaggagacg aaccatgagc
      961 agcagcctgg agacagagac gagagaagga ctggaagggg tcgtggcggc cacgacccgt
     1021 ctgagcagtg tcgacggcga ggccggcgtg ctgctgatcg ccggcttccc ggtggaagac
     1081 ctggcgccgg tggcgagctt cgaggaggtg gtgtacctgc tgtggaacgg cgccctgccg
     1141 cggccggacg agctggccgc cttcgcgcgg cggatggcgg cgcggcgggc gctccccggg
     1201 gcggccctgg acctgctgcg ggcggcggcc gggcggcggg tgccggtgat ggacgcgctg
     1261 cggatggcgg cgggcaccct gagcctgctg cccggcacgg gcgaggacgc gcgggaggac
     1321 ggcctgctgc tggtggcggc cttccccacc atcgtggcct cgtactggcg cctgctgaac
     1381 agcgaggagc cggtggagcc gcgggaggac ctcgggcacg ccgccagctt cctgcatctg
     1441 ctgtcgggcg aggagccggc ggccgagcgg gcgcggggtg tcgagaccta cctcaacacc
     1501 gtagtggacc acggcttcaa cgcctccacc ttcacggcgc gggtgatcgt gtccacgcgc
     1561 tcggacctgg tgtcggcggt gaccggggcg gtcggagcgc tcaagggccc cctccacggc
     1621 ggcgcccccg gccccgcgct ggacatggtg ttcgagatcg gcagcgccga gcgggccgag
     1681 gcggtgctgc gcgacaagct ggcccgcggc gagcggctga tgggcttcgg ccaccgggtc
     1741 taccgggtgc gggacccgcg ggcggacgtc ctggccgaag cggccgagcg cttctataag
     1801 gacaaggggg accgcggcgt ctacgagctc gcccgtgctg tggaggcgac ggccctgcgc
     1861 ctgctgcggg agcacaagcc ggaccgccgc atcgacacca acgtggagtt ctacacggcg
     1921 ctgctgctgc acggcctggg tctgcccacg gagctgttca cccccgcgtt tgccatcggc
     1981 cgcgtcgccg gctgggtcgc ccatggcctg gagcagctcg cccacggtcg cctgatccgc
     2041 ccgcagtcgg cgtacgtcgg cgagcgggac cgccgctggg tgccggtgga ggcacggtag
     2101 ggaaggtgct tttccctggg tgaagtttcc ggcggcggat cggaagctcc ggcggctcgt
     2161 cagagatcct tgcagggcgt cagaccttcc ggcggcacgc cggcaggttg cggcggcccg
     2221 ccggaggctc cgagaacccg ccggaaactc tgacggctcg ccgaagcttc cacagcctgc
     2281 cgaagatacg gcgacgcgcc ggagatcacg acagcgcgtc gaaaactccg atggctcgct
     2341 ggaagctctg acagcgcgcc ggaagcatcg gccgcacgcc gggagctccg acaaagcgcc
     2401 ggaagctccg acaacaagcc ggatcaggcc ccggcgggca gctcggcgag gagctttccc
     2461 agggcggcgg ggtcgacggg cttggtgaga tggaggtcga agccggcttc ccgtgagcgc
     2521 cgctgatcct cctcctggcc gtagccggtg agggcgatca gcttcgccga ggcgggcagg
     2581 ccatcgcggc gcagctcccg cgccacggcg tagccgtcca tgtccccggg caggccgatg
     2641 tcgcagagca cgacgctggg ctggaagcgg tgcgcctcgg acacgccgga cgggccgtcg
     2701 aaggccgtcg tcacctggtg gccggccagc tcgagcagga gctgcatgct ctcggccgcg
     2761 tcgacgttgt cctcgatcac caggatgcgg tggaggccgg gcggggcggg cgccgcctcg
     2821 ggccggctgc cggccggctc cgccgcctcc cggcgcggga gccgcagggt gaacaccgag
     2881 ccgcggccca gcccgtcgct ggcggcgtgg acctcgccgc cgtgcaggtc gaccagcccc
     2941 ttgaccagcg cgaggcccag gccgaggccg ccgcggctgc ggtcgaggct gtggtcggcc
     3001 tggctgaagg cctggaacag ccgctccacc atgcgcggct ccatgccgat accggtgtcg
     3061 cgcagggtga tcccgacaaa agcggccgcc ggatcggcca ccatctccag ctccacccgc
     3121 ccgccggcgt cggtgaactt gccggcgttg tgcaggaggt tgccgagcac ctgcgacagc
     3181 cgggtcggat ccccctggac ccagaccggc ccctccggca ggcggaccga gaggtgcagc
     3241 ccggccgcct ccaggtcgct gcggtggtcc tccgccgccg tccgcgccag cgccaccagg
     3301 tcgagggcct cctggcgcag caggatcttg ccgcgcgaga tccgcgatac gtcgagcagg
     3361 tcgtccacca gacgggcgag gtgggagacc tgccggtcga tcatgtcgcg cccccgctcg
     3421 atctcgggca ccccggagcc gagctggcgc atgacgaaga cggcgttgcg gatgggggcg
     3481 agcgggttgc gcagctcgtg gccgagcatg gccaggaact cgtccttgcg ccggtcggcc
     3541 tccttgagcg cctcctccgc ctgctggagc tcctgcacca gctcctccag acgccggcgg
     3601 gcccgcaccg gctcggtgac gtcgacgccg tggacgaaga tgccggagac cttctcgccc
     3661 gggtcgagca gcggcaggta gacgaagtcc acggcccgct cgtcgagcgg gccacccggc
     3721 tcgcgctgca gatggatggg cctctcacgg ccggtgaagg actcgcccgt ccggtagacc
     3781 tggtccagca gctcgaagaa cccctgcccc gcgagctccg gcagcgcctg ccggaccggc
     3841 ttgccgagga tgtcgcggaa gccgacgagc tgctcgaagg tcgggttcac cagctcgaac
     3901 acctggtccg ggccgcgcag ggtggcgatg aacgccggcg ccttcataaa cacccgggcg
     3961 aggcgcgagc gctcgctctc caggatacgg aagagctgcg cccgctcctc ctcgatccgc
     4021 tgctggtcgg tgagccgccc ctccagctcc gccagggccg cgtccttctc ccggcac
//
LOCUS       DASPJI010000357         5516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_830360, whole genome shotgun sequence.
ACCESSION   DASPJI010000357 DASPJI010000000
VERSION     DASPJI010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830360"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="VEL74_09500"
     CDS             <1..1456
                     /locus_tag="VEL74_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG62803.1"
                     /translation="APMAGTAGGTRAEQGPVTGPVPLTPIQSWYFAQEPSEPWHDNQA
                     LILASTRCLDPVRLDAALARTAVHHDALRLRFRSGDEGWEQWLDAPAPVSPLLAVDLS
                     AVPAAARSRQVEEVAALAQRSLNLTAGPVHRPVLFQGGGEREGWPDRFLWVIHHLAVD
                     GVSWRLLLEDLAAAYDGRALPPKTTSFRLWSERLVELSGSEVVRREAGWWLERPWNEV
                     APLPVDGPDSGAGSVADERSVAIALGPEETRALLEEVPRAYNSQINDALLSALARAFH
                     AWSGSPLLLVDLEGHGREDLFPDVDLSRTTGWFTSVFPVLVDLRQGADPGAALKTTKE
                     LLRAVPNGGLGWGLLARSPEAERLAGLPRPQVSFNYLGQVDQGLPQDLPFFPAAESAG
                     ANRSRQAPRAHLLEWNGSVSGGRLRFLCTYAEGAHRAASIERLAAGFAKALRELIAHC
                     RAPEAGGYTPSDFPLAGLSQGQLDKLLTRRKRPQ"
     gene            1453..>5516
                     /locus_tag="VEL74_09505"
     CDS             1453..>5516
                     /locus_tag="VEL74_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62804.1"
                     /translation="MSSAPSIEDIYPLSPMQQGMLFHLLDEPEAGLNVIQMSCALRGS
                     LDVDAFRRALGRVIERHAVLRTSFLWEEVDQPLQVVDREVPVPLAVEDWSGLGVEERR
                     HRLEEYLESDLARGFDLTQAPLMRMLLAREEADLHRLVWSIQLILFDGWSLPAVIGEV
                     LFHYEAFRAGDEPRLPTPRRYRDYIAWLLQREAGDGERFWRRELAGFTEPTPLPGDRG
                     PGRRPAMAQRRVFLPEPDTAALQALARRERLTLNTVVQAAWALLLARAGGLDEVVFGA
                     TVSGRPHDLPGVEGMIGLFINTLPVRVRLDPGGDPVEWLRGLQERQSEARQHEHVPLV
                     DVQGWSEVPRGLPLFESIVVFENYPVSEAVAGGRGTLELDEIRVLDANSYPLTLEIQP
                     GARLSLRLTYDGSRFDLRTIEELLSRLGDLLRQLAAGGARLSDLLLTAAERQQLAKWN
                     DTAVPRPPGLSLHGLFLARAATAPDAVALSFEGAGVSYGELRRRAGLLARRLRSLGCG
                     PETRAAVALERSPELVIALLGVLESGAAYVPLDPEQPAERLGRMLEDARPVALLTRAD
                     LLPRLPETAVPTLLFAGEEPGRRSPGEPPALGDDDQLAYVIFTSGSTGRPKASMLHHR
                     GIVNRLLWMAETYGLSPADVVLQKTPVGFDVSVWELFTPLATGGRLVLARPDGHREPD
                     YLAGLIAREGVTVLHFVASMLRSYLDTPDLPACPSPRLLVASGEALTADLVARYGGRF
                     PAAALENLYGPTETSVEVSAWSCAGQEDGPVPIGHPVANVRLHLIGRHGEPAAVGAPG
                     ELWIAGVAVGRGYLGRPDLTAERFVPDPFPGLPGDRGYRTGDLARRRPDGAIEFLGRI
                     DQQVKIRGFRIEPEEIAAVLTSHPAVRQAVVMAVEGDGGGPRLVAWVVAKDGDGAGVA
                     ELRQHLRGHLPEPMVPELIVPLAALPLTASGKVDRRALPLPETWDGGAAAGEHVAPRN
                     GFEEILADIWAAVLGRERVGAQDDFFALGGHSLLGVQVISRVRAAFGVELPVRALFNA
                     RTIAGLAALVEEATRGGAAPLPPLVAEPRGEGGLPLSFAQQRLWFLHQLDPDSTAYNM
                     PVAVRLAGELDRPALERALREVVRRHEALRTTFRQVDGRAVQVIAPWPDLALPLVDLA
                     GLGTAREAEALRLATSESRRPFDLERGPLFRPVLLRVAAGDHIVSATMHHIVSDERSL
                     EVFVREVTALYAAFGTGLPSRLPELPVQYGDYAVWQRRWLAGAAMEGELAFWRRELQG
                     LPPVLELPADRPRPEVRSTRGGSLDRDLGAAPATRLRDLARELGATPFMVALAAYQAF
                     LGRFAGVEDLAVGVPVAGRTLQEVEDLIGFFVNTL"
BASE COUNT          640 a         2001 c         2037 g          838 t
ORIGIN      
        1 cgcgcccatg gccggtaccg ccggcgggac ccgggccgag caggggccgg tcaccggccc
       61 ggtgcccctc accccgatcc agagctggta cttcgcccag gagccgtccg agccatggca
      121 cgacaatcag gcgctgatcc tggcgagcac ccgctgtctg gacccggtcc ggctcgacgc
      181 ggcgctggcg cggaccgccg tccatcacga cgcactgcgt ctccgcttcc gcagcggcga
      241 tgagggttgg gagcagtggc tggacgcccc ggcgccggtc tctccgctcc tcgccgtgga
      301 cctttcggcg gtgcccgcgg cggcgcgctc ccgtcaggtg gaggaggtgg ccgctctcgc
      361 ccagcgcagc ctgaatctta ccgccggccc cgtccatcgc ccggtgctct tccagggcgg
      421 tggcgagagg gaggggtggc ccgaccgctt cctctgggtg atccaccacc tggcggtgga
      481 cggcgtctcc tggcggctgt tgctagagga cctggccgcc gcttatgacg ggcgcgcgct
      541 gccgccgaag acgacctcgt tccggctctg gtcggagcgc ctggtggagc tctccggctc
      601 cgaggtggtg cggagggagg ccggctggtg gttggagcgc ccgtggaacg aggtggcccc
      661 gctgccggtg gacggcccag actcgggagc cggcagcgtc gcggacgagc ggagcgtcgc
      721 catcgccttg ggtcccgagg agacccgcgc ccttctggaa gaggtgccgc gggcctacaa
      781 cagccagatc aacgacgccc tgttgtcggc cctggcgcgg gccttccacg cctggtccgg
      841 ctcgccgctg ctgctggtcg acctggaggg gcacgggcgc gaggacctct tccccgatgt
      901 cgacctgtcg cgcaccacgg gttggttcac cagcgtcttc cctgtgctcg tcgacctgcg
      961 ccagggggcg gaccccggcg ccgccctgaa gaccaccaag gagctgctgc gcgccgtccc
     1021 caatggcggt ctgggttggg ggctgctggc ccgctcgccg gaggcggagc ggctggcggg
     1081 cctgccgcgg ccgcaggtga gcttcaacta cctgggacag gtcgaccagg gcttgccgca
     1141 ggatctgccg ttctttcccg ccgccgagtc cgcgggcgcc aaccgcagcc ggcaggcgcc
     1201 gcgggcgcat ctcctggagt ggaacggcag cgtctccggc ggccggctcc gcttcctctg
     1261 cacctatgcc gagggggcgc accgcgccgc cagcatcgag cgcctcgccg cgggcttcgc
     1321 gaaggcgcta cgcgagctca tcgcccattg ccgcgcgccc gaggccggcg gctacacccc
     1381 ctcggacttc cctctggccg gcctgagcca agggcagctc gacaagctcc tgacacggag
     1441 gaagaggccg cagtgagctc ggcgccttcg atcgaggaca tctaccccct gtcgccgatg
     1501 cagcagggga tgctgttcca tctcctggac gagcccgagg cggggctgaa cgtcatccag
     1561 atgagctgcg cactgcgcgg cagcctggac gtggacgcct tccgtcgggc cctgggcagg
     1621 gtcatcgagc gccatgccgt gctgcgcacc tcgttcctct gggaagaggt ggaccagccg
     1681 ctgcaggtgg tggaccggga ggtgccggtg ccgctggcag tggaggactg gagcggcctc
     1741 ggcgtggagg agcggcggca tcggctggag gagtacctcg aaagcgacct ggcgcgcggc
     1801 ttcgacctga cccaggcgcc gctcatgcgg atgctcctgg cgcgtgagga ggcggacctc
     1861 caccgcctgg tgtggagcat ccagctcatc ctcttcgacg gctggtcgct gcccgcggtc
     1921 atcggcgagg tgctattcca ctacgaggcg ttccgggccg gtgacgagcc gcgcctgccg
     1981 acgccgcggc gctatcgcga ctacatcgcc tggctgctgc agcgcgaggc cggcgacggt
     2041 gagcgcttct ggcgccgcga gctggccggc ttcacagagc cgacgccgct gcccggcgac
     2101 cgcggccccg gacgtcggcc cgcgatggcg cagcggcggg tcttcctgcc cgagccggac
     2161 accgccgccc tgcaggcgct ggcacggcgc gagcggctga ccctcaacac tgtggtgcag
     2221 gccgcctggg cgctgctcct cgcccgcgcc ggcgggctgg acgaggtggt cttcggggct
     2281 acggtctccg gccggccgca tgacctgccc ggcgtcgagg ggatgatcgg cctcttcatc
     2341 aacaccctgc cagtgcgcgt ccgtctcgat ccggggggcg acccggtgga gtggctgcgc
     2401 ggacttcagg agcgccagtc cgaggcgcgc cagcacgagc acgtgccgct ggtcgacgtc
     2461 caggggtgga gcgaggtgcc ccggggcctg ccgctcttcg agagcatcgt ggtgttcgag
     2521 aactaccccg tgtccgaggc ggtggcggga ggccgcggca ccctcgagct cgatgagatc
     2581 cgggttctgg acgccaacag ctatccgctc accctggaga tccagcctgg agcgcggctc
     2641 tccctgcgcc tcacctatga cggctcccgc ttcgatctcc ggaccatcga agagctgctg
     2701 tcgcggctcg gcgacctcct ccgccagctg gccgcgggcg gcgcgcggct ctcggacctc
     2761 cttctgaccg ctgccgagcg ccagcagctt gccaagtgga acgatacggc ggtgccacgg
     2821 ccgccagggc tctccctgca cggcctcttc ctggcgcggg cggcgaccgc cccggatgcc
     2881 gtcgccctca gctttgaggg cgccggcgtc tcttatggcg agctgcgccg ccgggcgggt
     2941 ctcctcgccc gccgcctccg ctccctgggc tgcggccccg agacccgggc cgccgtcgcc
     3001 ctggagcgct cgccggagct ggtgatcgcc ctgctcggcg tactcgagtc gggggcggcc
     3061 tacgttcccc tggaccccga gcagccggcc gagcgtctgg gccggatgct ggaggacgcg
     3121 cggcctgtcg ccctcctcac ccgggccgac ctgctgccac gcctgcccga gacggccgtc
     3181 cccacgctcc tcttcgccgg cgaagagccc gggcggaggt cgccggggga gccgccggct
     3241 ctgggggatg atgaccaact cgcctacgtg atcttcacct ctggctccac cggccggccc
     3301 aaggcttcga tgctgcacca ccgcggcatc gtcaaccgcc tgctgtggat ggcggagacc
     3361 tacggcctct cgcctgccga tgtggtgctc cagaagacgc ccgtcggctt cgacgtctcg
     3421 gtttgggaac tgttcacgcc gctcgccacc ggtggccggc tggtgctggc gcggcccgat
     3481 gggcaccgtg agcccgacta tctggccggg ttgatcgccc gcgagggggt gaccgtcctg
     3541 cacttcgtcg cctccatgct gcggtcctac ctcgacacgc cggacctgcc ggcctgcccg
     3601 tcgccacgcc tgctggtggc gagcggcgag gcgctgaccg cggacctggt ggcccgctac
     3661 ggcggccgct tcccggcggc ggcgttggag aacctctacg gtccgaccga aacctcggtg
     3721 gaggtgagcg cctggagctg cgccggccag gaggacggcc cggtgccgat cggccaccct
     3781 gtggcgaacg tgcgcctgca cctgatcggc cggcatggcg agccagcggc tgtcggcgcg
     3841 ccgggggagc tgtggatcgc tggtgttgcg gtgggccgcg gctatctcgg gcgtcccgac
     3901 ctcaccgccg agcgtttcgt ccccgatccg ttccccggcc tgccgggtga ccgcggctac
     3961 cgcaccggcg acctggcgcg gcgccggccg gacggcgcta tcgagttcct cggccgcatc
     4021 gaccagcagg tgaagatccg cggcttccgt atcgagccgg aggagatcgc ggcggtcctg
     4081 acctctcacc ccgccgtgcg ccaggcggtg gtgatggcgg tggagggcga cggcggcggc
     4141 ccccgcctgg tcgcttgggt agttgccaaa gatggagacg gtgcgggcgt ggcagagctg
     4201 cggcagcacc tgcgtggcca ccttcccgag ccaatggtgc cggagctgat cgtgccgctg
     4261 gcggcgctgc cgctcaccgc cagcggcaag gtagaccggc gcgcgctgcc gctcccggag
     4321 acgtgggacg gcggggcggc cgccggcgag cacgtcgccc cgcgcaacgg cttcgaggag
     4381 atcctcgccg acatctgggc cgcggtgctc ggccgcgagc gggtcggggc ccaggacgac
     4441 ttcttcgccc tcggcggcca ttcgcttctc ggcgtgcagg tgatctcccg ggtgcgcgcc
     4501 gccttcggcg tcgagctgcc ggtgcgcgcc ctgttcaatg cccgcaccat cgccggcctc
     4561 gcggccctgg tcgaggaggc gacgcgcggc ggcgccgcgc cgctgccacc gcttgtggcc
     4621 gagcctcgcg gtgagggggg gctgccgctc tccttcgcgc agcagcggct ctggttcctg
     4681 catcagctcg atccggactc gacggcctac aacatgccgg tcgcggtgcg gctggccggg
     4741 gagctcgacc gtcccgccct cgagcgggcg ctgcgcgagg tggtgcggag gcacgaggcg
     4801 ctgcgcacca ccttccggca ggtggacggc cgggccgtgc aggtgatcgc accatggccg
     4861 gacctggcgc tgccgctcgt cgacctggcg ggcctgggca cggctcgaga ggccgaggcc
     4921 ctgcggctcg ccacgtcgga gtcccggcgg cccttcgatc tcgagcgcgg gccgctgttc
     4981 cggccggtct tgttgcgcgt cgcggcgggc gaccacatcg tgtcggctac catgcaccac
     5041 atcgtcagcg acgagcggtc gctggaggtt ttcgtccgtg aggtgacggc cctctacgcc
     5101 gccttcggta ccggcctccc atcgcggctg ccggagctgc cggtgcagta cggcgactac
     5161 gcggtctggc agcggcgctg gctcgccggc gccgcgatgg aaggcgagct ggcgttctgg
     5221 cgccgcgagt tgcaggggct gccgccggtc ctggagctgc cggccgaccg gccgcgtccc
     5281 gaggtgcgga gcacccgcgg cgggtcgttg gaccgcgacc tgggagcggc gccggcaacg
     5341 cggctgcggg acttggcgcg ggagctgggg gcgacacctt tcatggtggc actggccgcc
     5401 taccaggcct tcctcggccg cttcgccggg gtggaggact tggccgtggg cgtgccggtg
     5461 gcggggcgca ccctgcagga ggtcgaggat ctcatcggct tcttcgtcaa caccct
//
LOCUS       DASPJI010000358         4793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_592986, whole genome shotgun sequence.
ACCESSION   DASPJI010000358 DASPJI010000000
VERSION     DASPJI010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592986"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VEL74_09510"
     CDS             <1..1017
                     /locus_tag="VEL74_09510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007356797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG62805.1"
                     /translation="SPSLPSGVPISIVVATRDRPEDLRRCLASLVAQESPRPVEIVVV
                     DNHPGSGQTPPVVAEFPGVVLVSETRAGLSYARNRGFLVSRGEILVTTDDDVVVPPGW
                     LEALLAPFARADVAAVTGNVLPLELETGSQRLFEAYGGLGRGYRRREYGRDFFDRFRR
                     AVPTWELGATANAAFRASLFADPRIGLLDEALGAGMPPGVGEDTLLFYRLLKEGHTLI
                     YEPSAWVWHRHRADDRAFRRQIFAYSKGHVAYHLTTLLRDGDPRALVRLLFELPRAHA
                     SRILRRLLGKSPYPVSMILLEIWGNLLGPLALLQARLRVRRWGRSGEPRGMDAPGDPV
                     VTRS"
     gene            1024..4749
                     /locus_tag="VEL74_09515"
     CDS             1024..4749
                     /locus_tag="VEL74_09515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG62806.1"
                     /translation="MDVTVVIPARNAAETLAETLDSVLSQSFGGWEAVVVDDGSTDAT
                     AEVADAFARRDPRVRLVRQPPLGVGMARNTGLAEARSDWLLFLDADDWLCPRHLERMT
                     AILAAEEASGSEGIGGAYCGWRRVAPGGLPGPALHAPADDDLFHVLARGCAFAIHACV
                     VRRGLVEAVGGFDAGLVTCEEWDLWQKVARTGARFLRVPEVLALYRMRAGSASLDPHR
                     LLADGLRVIGRGHAPDPRVPSAAAHYIEGVSRQNLPAARLLFLCWCAGLAIGRGDDPL
                     PLLASVPMTAGLPDPPLDPTAAARSLFLTTVLPRGLPPESWLDLWPEVEGSLGALLAL
                     LGRRTSTPAFARRTRRVLERLILEHAPAPTAGGTTLGSTRVEPVEITEPFQDITVPIS
                     VDRLHARVLLEGEPLGSLELPVCDRVVPAAVLADAVAAEYAWQILGRFIERTTQRPEP
                     APLISPSPLTAWQQARRRTAVRGAGEEARPASAPAASGENGSRGDFDRTGWTSFLQEI
                     WGRPGWPVSHFYDPQAVREDDEPRRRAERGWIVVEVAGELPRVEIPHGETLEELRVVL
                     RVGGVETGIVPVAVRGEISAHELRVALTEAAGFELCRAAVREALLGVPLGRPGNLRER
                     LAAASARHRRETPSSAGPPEAWPLAGSEPAPGWQQTLTRAVGPEGRGAVLARHASVRR
                     AALPSAALLDLEESAAVAGEPLLRVEGTASTPGPPRIVYAPDLLWVPHPPAPSRASPG
                     KGEDGERVDREGVPYGRSHFEALFASGQDPWRYTSAYEATKYDQTLDLLDGLTGSGPI
                     PRALELACAEGHFTVRLAPRVGHLVAVDISRIALERAAQRCQGLDNVELHALDLFRDP
                     LPGPFPLIVCSEVLYYAGGLEDLARTASSLAGALEPGGHLLTAHANLIVDEPDRPGFD
                     WDHHPFGAKTISDALEAAGLHRIRELRTPLYRVQLFRRPRGLLSVLPPRVQLTETDEL
                     PPLPPKVAERARLQGGTLLSRQVNEAAGVLPGVVTYRLPILMYHRVASGGGAGDPARW
                     RVSPEAFEEQLRYLRDSGYHTPRLEDWRRAAESKRPLAGRAVLLTFDDGYRDFAEHAW
                     PLLRRYGFSALVFLVAGAVGSTNRWDAGYGHGLPLLGWDEVRRLRAEGVELGSHSGTH
                     RPLTSLAATEVVREAAHSRAVIARALGEPPRAFAYPYGDTDPAVRHLVSACGYVYGLT
                     TRPGRCGFGDGMLDLPRIEVAGDAPFERFVARLEEVS"
BASE COUNT          532 a         1897 c         1685 g          679 t
ORIGIN      
        1 tcgccgtccc tcccctctgg ggtccctatc tccatcgtcg tcgccacccg cgaccggccg
       61 gaggatctcc gccggtgcct cgcttctctg gtcgcccagg agtcaccgcg cccggtggag
      121 atcgtggtgg tggacaacca ccccggctcc ggccagaccc cgccggtggt ggcggagttc
      181 cccggcgtcg tcctggtgag cgagacacgg gccggactct cctatgcccg caaccgcggc
      241 tttctggtca gccgggggga gatcctggtg accaccgacg acgacgtcgt ggtgccgccg
      301 ggctggctcg aggccctcct cgcccccttc gcccgggccg acgtggccgc ggtgaccggc
      361 aacgtcctgc cgctggagct ggagaccggg tcgcagcgcc tgttcgaggc ctatggcggg
      421 ctgggccggg gctaccggcg gcgcgagtac ggccgcgact tcttcgaccg cttccggcgc
      481 gccgtgccca cctgggagct gggggccacg gccaacgccg ccttccgcgc ctccctcttc
      541 gccgacccgc gcatcggcct cctcgacgag gccctcggcg ccggcatgcc ccccggcgtc
      601 ggcgaggaca ccctgctctt ctatcgtctc ctcaaggagg gtcacaccct gatctacgag
      661 ccttcggcct gggtctggca ccgccaccgc gccgacgacc gggccttccg gcggcagatc
      721 tttgcctaca gcaaggggca cgtcgcctat cacctgacca ccctgctccg cgacggcgac
      781 ccccgcgctc tggtgcgcct cctcttcgag ctcccccgcg cccacgcctc gcgcatcctc
      841 cggcgcctcc tcggcaagag cccctacccg gtgtcgatga tcctcctcga gatctggggc
      901 aacctcctcg gaccgctggc cctgctgcag gcccggctgc gggtgcggcg ctgggggcgg
      961 agcggagagc cgagggggat ggatgcgccc ggcgaccccg tggtgacgag gtcctgaacg
     1021 gccatggacg tcaccgtcgt catcccggcc cgcaacgccg ccgagaccct cgccgagacc
     1081 ctcgattcgg tgctctccca gagcttcggc ggctgggagg cggtggtggt ggacgacggt
     1141 tcgaccgatg ccacggcgga ggtggcggac gccttcgccc gccgtgaccc ccgcgtccgc
     1201 ctggtccgcc agccgcccct gggggtgggc atggcacgca acaccggctt ggccgaagcc
     1261 cggtccgact ggctcctctt cctggatgcc gacgactggc tctgcccgcg gcacctcgaa
     1321 cggatgaccg ccatcctcgc cgccgaggag gcctccggct cggagggaat cggcggcgcc
     1381 tactgcggct ggcgccgggt ggcgcccggg gggctgcccg gacccgcgct ccacgccccc
     1441 gcggacgatg acctcttcca cgtgctggcg cgcggctgcg ccttcgccat ccacgcctgc
     1501 gtcgtccgcc gcggcctggt cgaggcggtc ggcggcttcg acgcagggct cgtcacctgc
     1561 gaggagtggg acctctggca gaaggtggcg cggaccggcg cccgcttcct ccgcgtgccc
     1621 gaggtgctgg cgctctaccg catgcgcgcc ggctcggcct ccctcgatcc ccaccggctg
     1681 ctcgccgacg gcctgcgggt catcggccgc ggccacgcgc cggacccgcg ggtccccagt
     1741 gccgcggccc actatatcga gggggtctcc cggcagaacc tgccggcggc gcggctcctc
     1801 ttcctctgct ggtgcgccgg ccttgccatc ggacgggggg acgacccgct ccccctgctc
     1861 gcttcggttc cgatgacagc ggggcttccc gatccacccc tcgatcccac ggccgcggcc
     1921 cggtctctct tcctcaccac cgtcctgccg cggggactgc ccccggagtc ctggctcgac
     1981 ctctggccgg aggtggaggg ctcgctgggc gcgctcctcg ccctcctggg ccggcggacc
     2041 tccacgccgg cgttcgcgcg ccgcacccgg cgggtcctgg agaggctgat cctggagcac
     2101 gcgccggctc ccacggccgg cgggacgacc ctcggcagca cccgggtgga gcccgtcgag
     2161 atcaccgagc cgttccagga catcacggtc cccatctccg tcgaccgtct ccacgcccgg
     2221 gttctcctgg agggtgagcc cctgggcagc ctggagctgc cggtctgcga ccgcgtcgtg
     2281 cccgccgcgg tgctcgccga cgccgtggcc gccgagtacg cttggcagat cctgggccgc
     2341 ttcatcgaac ggaccacgca gcggccggag ccagcgcctc tgatctcccc ctcccccctc
     2401 accgcctggc agcaggcccg gcgccgtacc gcggtccgcg gggccgggga ggaggcccgg
     2461 ccggccagcg cgccggcggc gtccggcgag aacggctccc gcggcgactt cgaccggacc
     2521 ggctggacct ccttcctgca ggagatctgg ggacggcccg gctggccggt ctcccacttc
     2581 tacgatcccc aggccgtgcg ggaggatgac gagccgcggc gccgggcgga gcgcggctgg
     2641 atcgtcgtcg aggtggcggg cgagctgccg cgggtggaga taccccacgg ggagacgctg
     2701 gaagagctgc gggtggtcct ccgggtgggc ggagtcgaga ccgggatcgt gccggtggcg
     2761 gtccgcggcg agatctccgc ccacgagctg cgggtggcgc tcaccgaggc ggcgggcttc
     2821 gagctctgcc gcgccgccgt gcgcgaggcc ctgctcggcg tccccctggg ccggccgggc
     2881 aacctccggg agcgtctcgc cgcggcgtcc gcccgccacc gccgcgagac gccctcgtcc
     2941 gccggcccgc cggaagcctg gcctctggcg ggctccgagc cggcgccggg ctggcaacag
     3001 acgctgaccc gcgccgtggg cccggagggc cgaggcgcgg tcctggcacg gcatgcctcc
     3061 gtccgccggg cggccctgcc ttcggcggcc ctgctcgatc tggaggagtc ggccgccgtc
     3121 gccggggagc cgctgttgcg ggtcgagggc acggcgagca ccccgggacc gccacgcatc
     3181 gtctacgcgc cggacctgct gtgggtccct caccccccag ccccctctcg cgcctccccg
     3241 ggaaaggggg aggacggcga gagggtggac cgggaggggg tgccctacgg ccgctcccac
     3301 ttcgaggcgc tgttcgcgtc cggccaggac ccctggcgct acaccagcgc ctatgaggcc
     3361 accaagtacg accagacgtt ggacctcctc gacggcttga cggggagcgg ccccatcccg
     3421 cgggctctgg agctcgcctg cgccgagggg cacttcaccg tccgcctggc tccccgggtg
     3481 ggccacctcg tcgccgtcga catctcccgc atcgccctgg agcgggcggc ccagcgatgc
     3541 cagggcctgg acaacgtcga gctccacgcc ctcgacctgt tccgggaccc gctgcccggc
     3601 ccgttccccc tgatcgtctg cagcgaggtc ctctactatg ccggcggctt ggaggacctg
     3661 gcgcggacgg cgagctcgct cgccggtgcc ctggagcccg gcgggcatct cctcaccgcc
     3721 cacgccaacc tgatcgtgga cgagccggac cgcccggggt tcgactggga ccaccacccg
     3781 ttcggcgcca agaccatcag cgacgccctg gaggccgcgg gcctgcatcg gatccgcgag
     3841 ctgcgcacgc cgctctaccg ggtccagctc ttccggcgcc cccgcgggct cctcagcgtc
     3901 ctgccgccgc gggtccagct caccgaaacg gacgagctgc cgccgctgcc gcccaaggtg
     3961 gccgagcgcg cccgcttgca gggagggacc ttgctctccc gacaggtcaa tgaagccgcc
     4021 ggggtcctcc cgggggtggt cacgtaccgg ctgcccatcc tcatgtacca ccgggtggcg
     4081 tcaggaggcg gcgcggggga tccggcccgc tggcgagtga gcccggaggc gttcgaggag
     4141 cagctccgct acttgcggga ctcgggctac cacaccccgc gcctggagga ctggcggcgg
     4201 gcggcggagt ccaagcgccc gcttgccggc cgggcggtcc tcctcacctt cgacgacggc
     4261 taccgcgact tcgccgagca cgcctggccc ctgctgcggc gatacggttt ctcggccctg
     4321 gtcttcctgg tggccggtgc ggtcgggagc accaaccgct gggacgccgg ctacggccac
     4381 ggcctgccgc tcctcggctg ggacgaggtc cgccgcctgc gggccgaagg ggtggagctc
     4441 ggctcccact cggggaccca ccgcccgctg acctcgctgg cggccaccga ggtggtgcgc
     4501 gaggccgccc actcccgcgc cgtgatcgct cgcgccctcg gcgagccgcc gcgggccttc
     4561 gcctacccct acggcgacac cgacccggcc gtccgccacc tggtaagcgc ctgcggctat
     4621 gtctatggcc tcaccacccg gccggggcgc tgcggcttcg gcgacggcat gctcgacctg
     4681 ccgcgcatcg aggtcgcggg ggacgccccc ttcgagcgct tcgtcgcccg cctggaggag
     4741 gtctcgtgat ccgcccgccg accaccggca tcaccgtcgt ggtctgcacc cgg
//
LOCUS       DASPJI010000359        23406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_535878, whole genome shotgun sequence.
ACCESSION   DASPJI010000359 DASPJI010000000
VERSION     DASPJI010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 23406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23406)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23406
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535878"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2488
                     /locus_tag="VEL74_09520"
     CDS             <1..2488
                     /locus_tag="VEL74_09520"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYG62807.1"
                     /translation="RREARQLLAADLAIEGRRPLPPDFDLARAGLADVRRTDITELVT
                     VAAAPPRGSAPGPSQLVELKAIRGDYPFYGVLELRPDRPLAALLRADTVVVAAELLSR
                     LGLRVGDELRIGGQPFRIAGVVVSEPDRVSVSLTLGPRVFISADGLARTALIQRGSRV
                     GYRTLLKLPAGFPGAGVEPAAERLRLALPDTTFYEVESYRAAQPELRDNLSRVERYLG
                     LVALLSLFVGGIGVAQSVRAWLASRLDAIAVLKCLGLRPREIFPLYLGQTALLGLAGS
                     VVGIVAGAAVQLALPSLFPDLIPAELIRPWQPASLLRGLGLGLGVALLFSLPPLSAVL
                     RVPPARVLRRDAEPLPRHRWAAAVTGVALVAGVWGMATLQSRSWDLGARFTGGVALVT
                     VVLAAAALLVSRGAQRIPRDLSPSWARLWLRHGLAALARPGAGTVGAIVAMGLGVLVV
                     LGMSLVERRLSRELSTAIPENAPSAFLVDIQPAQWPGIEGVLRQAGATRIDSVAVVMA
                     RLSAIDGKGIEDLAAPEPAPAEEAGTQTNNRPDERARQGRGRRRGGEDGARRWALTRE
                     QRLTYMKELPADNEIVAGALWSDPARAEVSVEKDYADDLDLKLGSVLRFDIQGVPVEV
                     TVTSLRTVDWGTFGINFFLVVEPGVLEEAPQQRLTAVLLPRGAEQRVQDEIAANYPNV
                     TMLRIREILEKVLGVMKRIGLAIRFLGAFTVLAGIAILAGAISAGSARRGREVALLKT
                     LGMTRRGVAATFAVEYALIGLVAGVIGALGAAVLARGVVTQGFELTWQAEPGPVTLAI
                     LASVVLTVAAGLAASFRALERRPIEVLRSE"
     gene            2513..3370
                     /locus_tag="VEL74_09525"
     CDS             2513..3370
                     /locus_tag="VEL74_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYG62808.1"
                     /translation="MDGLTSRTIPTNGIHLHAVEAGPADGPLVVFLHGYPEFWYAWRK
                     QIAPFAAAGFRVLAPDLRGYNLSDKPKGISSYRIDHVARDVLGLIDEAGRDKAFIAGH
                     DWGGVAAWWLGMAHPDRVEKLGLLNIPHPAVARQHILKNGKQRRRSWYIFFFQLPWLP
                     ERAFKARNFTFAAKSLRGSARRGTFTDEDLARYREAWRQPGAARSMLNWYRAAPWAPT
                     AKLPSQRVTVPTLLLWGAKDIALGRELAQPSIDRCDDGRLVFIEEAGHFVQHEEPERV
                     NGMLGDFFS"
     gene            4034..4246
                     /locus_tag="VEL74_09530"
     CDS             4034..4246
                     /locus_tag="VEL74_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62809.1"
                     /translation="MRKKMLLLALALTAAASLSIQRAEAAGGYSCPQCVTYADGSRCC
                     VSCWCNSSGFPIACTDNYCPPPGGIN"
     gene            4598..4888
                     /locus_tag="VEL74_09535"
     CDS             4598..4888
                     /locus_tag="VEL74_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HYG62810.1"
                     /translation="MSEQEIRSRIKQIISNIAGLDPQRIGDDDSFTGDLSLDSLSMLE
                     IGVDVDMAFQLGLPDERYKEVRTLPQMVALVQQRRSEIGGGVPAGALQEAVS"
     gene            4888..6114
                     /locus_tag="VEL74_09540"
     CDS             4888..6114
                     /locus_tag="VEL74_09540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014179313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HYG62811.1"
                     /translation="MHGRIAVTGLGWVTPIGAGRDEVWEGLLAGRSGFAPVESFDTAR
                     FSVHVGAEVRGFDARPWIRRLDPGRLGRASQLAIAAARMALEDAGLADLDAESHQGRE
                     RTGVVMGTTSGEPREVERFDDRYLAGDTAAIGAEFMGLYPCHMIAAHIAREMGIRGPV
                     EMIPTACAAGNYAIARAFDLLRTGRSDLVLAGGADAFSRITYAGFHRLGAIAPERCQP
                     FDRDRKGMIPGEGAAVLVLEPLERARARGARVYAEVAGYGLSCDAHHMTAAHPEGDGA
                     ARAMLAALADAGLEARDVSYISAHGTGTPTNDRLETLAVQRVFGEAAARTPMSSIKSM
                     IGHTMGAASAIEAGVCSLAVATGRVPPTMNMEAPDGDLDYVPNAARELDVRVAMNNAY
                     AFGGNNASVLFRSTEH"
     gene            6116..7345
                     /locus_tag="VEL74_09545"
     CDS             6116..7345
                     /locus_tag="VEL74_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYG62812.1"
                     /translation="MRRVVITGAGVLSPLADSPAGLHAALCAGQSALKPVELFPLDGV
                     TPRPAGEIRPFDPKAYLGERNLRPVDRTSRLLLVAAQQALESAGWTAGERAAREVGLV
                     LGTTFCSVHTIAEFDRRAVQLGPSYASPMDFANSVINAAAGQAAIWHGLRGVNSTVSA
                     GEASGLLALAHAADLIRAGRAEALLAGGAEELCFESFLGCERAGRLGLSVTVPFDARR
                     DGFALAEGAALLMLEEAGSAAARGARVLAEVRGHGKAFNPAGAEDEASAVSAVARAVH
                     LALEDAAVGPTDLDVLSLSANGSVHGDHNEAWGVGMALDGAGDRPVAAIKAMLGEALG
                     ASGAFQAAALLGTFGDGVVPGVLGLERTEGGFPLAGVRAETRRLGRAPRLGLLHAMAW
                     DGQSCALVLEAPPEARP"
     gene            7342..8556
                     /locus_tag="VEL74_09550"
     CDS             7342..8556
                     /locus_tag="VEL74_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HYG62813.1"
                     /translation="MNGPERPGIVPRFEEQGYSAEAIAERRRWLTERTGAPLTHVGAF
                     TVPGEEMRGNIENPVGAVQVPLGVAGPLLVQGEHARGTFYVPLATTEGALVRSYERGA
                     AAITRAGGATVRLYADENRVSPVFLFDSVAEAADFVRGLPGWFEAVRAAAESTTRHGK
                     LLRLEPRPLGREVVVHFCYSTGDAHGMNMIVRATDHACRWLLANTPARRHLVFSGLEA
                     EKRAAGSLFAGGKGKKVVAGALVPDGICRAWLHATPGQLTEVWRHTLLGHVQGGTLGY
                     NGHFANGLTALFIACGQDVANVTNSAVGVTSFEVTGGGDLYASVTLPSLTVATVGGGT
                     GLGTARECLEVLGCAGDAARGGAPKLAEIAAALLLGGELSMAGAIASGEFVQAHEAYG
                     RNRPAAPVEAGA"
     gene            8553..10103
                     /locus_tag="VEL74_09555"
     CDS             8553..10103
                     /locus_tag="VEL74_09555"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYG62814.1"
                     /translation="MKRLFRGLRRPPDATYDAVVIGAGIGGLVTANLLAREGLRVLLV
                     EQHYMVGGYCSTFKRRGYVFDAATHFYPLLGNAETLTGRLLAELGATTGWVKMDPVDT
                     FHFPDGSRFEVPADFDAYLARLKAEFPEEAAALDSFFQAVRETYLLGLLHYFRGRPLS
                     QLGRLAPYRDLTVREALDRWFRDPKLKLLLTADCPHWGSPPGRTSFVFDSMLRLSYFL
                     GNYYPQGGSQAFADELARCFEERGGHVLTSAMAQRILIAGGTACGVELEILRGPLAGS
                     SFTVAAGAVVSNADLLLTLERLIGAAHVAPETLAGLRSLRPTYPCFLTYIGTAGIPDS
                     VLEDVQGYYWDGWDMDRVGTDALRFKIFAPTLYEPGMAPPGGQVILVQKVLEMDYGAV
                     TDWPAHKRQVEEQAFQHLERLIPGFSGHVVVTDSSSARSAWRFTLNHQGAMLGWEMSP
                     WQLGEGRPDIAGEIRNLYFTGHWVRPGGGITPVIVSAQQAAAAVLRGAGDVDTREELC
                     STLMTATS"
     gene            10073..11869
                     /locus_tag="VEL74_09560"
     CDS             10073..11869
                     /locus_tag="VEL74_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62815.1"
                     /translation="MQHANDCDVLILGAGLAGVCLARQILLADPEKRIIMVDRRSDLP
                     PTKQKVGEATVQMSGYYYAKVLELEEYLLQEHYLKYNLRFFWPSAQGADRYEELSQSY
                     IRGLSNIPTYQLDRNKFEGEVLRRNLADPRFTLYAPVKNIEIDLAETVAAGPHSFGFE
                     TGDGKVSGQAGWVVDATGRGRLLSKRRQLQRPSPIHHGTTFFWVDGLVDIERLTDLTP
                     KEVRLRPDRSTLGHTPAFLATNHFCGEGWWFWVIPLHNRTSLGLVYDNRLVPREEVAT
                     AEKTIDFVCRHYPLFERALRNREIIDASGFVSFAHDCGQTLSTARWAMVGEACRFTDP
                     LYSPGGDLISTYNTLITDAILTDDPRELESKVRLYEGLARAVYESYVPSFAVSYSTLG
                     DQECFSLRYTWELAIYFSFFVFPFINDLFTTRAFVPGFLQRFGRLGPMNRGVHELLAG
                     YYEWKKEHPRGPAREPLFFDFYELWPLKAAETCFYRVGVTPEEARQILDEHLGNLREN
                     ARWIYAYVAATVLGDERAVTHPEFVGGIDVDNLAFDPREMRERLERCGETDERWSWNF
                     EPPALCRFRKEVAAVEPMEEAEEPMMAAGGAR"
     gene            11866..13314
                     /locus_tag="VEL74_09565"
     CDS             11866..13314
                     /locus_tag="VEL74_09565"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HYG62816.1"
                     /translation="MITAQRPVLDPPPDLAPGLERAFAFVPEERSGPVQVEGSLPASL
                     RGTYYLNGPARFARAGLRYRHWLDGDGMVCALRFDDAGESGAHLTARFVRSAKWTAEE
                     EAGRPLFRAFGTAFPGDRLVRGVALESPVNVSVVPWRDTLLAFGEQGLPYELDPVTLE
                     TRGLYNFGGTLNPVSPLAAHAKVDPTNGELLDFGVSFASARPLLNLYRYSAEGALLYR
                     RRLPLPFPCSLHDFALSPRHAVFYLAPYVLDVERLLREGSTLMESLVWRPELGTRLLV
                     VDRQTGEAVADLPIGDRYCLHTINAFEEGDLLAVDVVELDRPVYDQYEVVPDLFTSVS
                     PGRPVRLLVDLRGGSLVERRELGPTEAPDFPAIAPCRTGLPTEDVWMLDISTAGRPGR
                     KFFDRLVHLPAGGTPDVWQAPPGVYLGGEPAFAPLAGEERGAVLCQRFDAVANESAFL
                     VFDAFGVAAGPRAVLPLESPLHLGFHAVFVPE"
     gene            13338..13895
                     /locus_tag="VEL74_09570"
     CDS             13338..13895
                     /locus_tag="VEL74_09570"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014446375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HYG62817.1"
                     /translation="MPDTTPVLYPETIHDPAAVRSRTVTWQDPALGLSRAREMSGLDW
                     LNEMFRGKLPRPPLNNLLGVDEGGAEPGRAWMSFPAGEHLYNPMGTVHGGVLSTVLDS
                     AMGCAVQSTLGPGEGYTTIDLHVTFVRPVTAASGRLRCEGEMVHAGRRLAMAQARLTD
                     AQGRLCAHAVSSCMIRRAGDHDGRV"
     gene            13916..15289
                     /gene="gdhA"
                     /locus_tag="VEL74_09575"
     CDS             13916..15289
                     /gene="gdhA"
                     /locus_tag="VEL74_09575"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HYG62818.1"
                     /translation="MHDYVASLVSEVKAKNPGEPEFHQAVAEVMESLALVLGRRPEYR
                     RAKILERLVEPERVILFRVPWQDDRGEVHVNRGFRVEMCSAIGPYKGGLRFHRSVNLG
                     ILKFLAFEQIFKNSLTTLPMGGGKGGSDFDPKGKSDDEVMRFCQSFMCELYRHIGPNT
                     DVPAGDIGVGGREIGYLFGQYKRLRNEFTGVLTGKGLHWGGSLIRPEATGYGCVYFAQ
                     EMLATRGQTLEGKTCLVSGSGNVAQYTVEKLLDLGARPVTLSDSDGFIHDEAGLDRDK
                     LAFVMELKNVRRGRIQEYAERYGTATYVPNGPGPAQGLGHNPLWDRQADCAFPSATQN
                     EINGRDAANLLRNGVFLVSEGANMPSDIDAVNRYLDAGILYGPGKAANAGGVATSGLE
                     MAQNSMRYRWTREEVDGKLQQIMKEIHRACLETAEAYGVPGNYVHGANIAGFLKVAEA
                     MMDQGLV"
     gene            complement(15742..16995)
                     /locus_tag="VEL74_09580"
     CDS             complement(15742..16995)
                     /locus_tag="VEL74_09580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG62819.1"
                     /translation="MAAIAVFAVRENRLLLLAVEALFALSATLGVLLVRSFFVPLELI
                     RTGAELMSEREFTSSFREVGQPEMDALVRVYNEMVARLREERLKLQEQHFFLERILAA
                     SPAGILTLDFDGRIAQANPSAAALLETSAEGGLLAASAGPLGAALAALRVGESRVLPL
                     QGGRRIKASRAEFFDRGFPRSFFLLEELTEELRASESAAYGKLIRMMSHEVNNSVGAV
                     GSLLDSFRGYAGQLGDEDRDDFLAALGVAIARMERLSAFMNGFATVVRLPEPDPRPTD
                     VRRLVDEILLLLRPELERRRVACAWERAEALAPIAPIPMDRNQMEQVLVNVFKNALES
                     IGEDGRIALRLEREGDGAVLAVRDTGPGIPAGVEPLLFTPFFSTKRDGRGLGLTLVRE
                     ILSRHGFAFGLRNAPAGGAELWIRF"
     gene            complement(17050..18483)
                     /locus_tag="VEL74_09585"
     CDS             complement(17050..18483)
                     /locus_tag="VEL74_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYG62820.1"
                     /translation="MILIVDDDPSVAASLGLLLKRHGYSTRAARSQEEALAAVRGQEP
                     GPDEIGLVLQDMNFSRGTDGEEGLELLAEIRRLRPHLPVILITGWGSIALAVEGMKAG
                     AADFITKPWSNAQLLQSVETALSLAASRSGADEGQGRTPSREELDRRFDFRGLVGNEP
                     RFLKLLEVVGRVSPTDASVLITGESGTGKELIAEAIHRNSRRRDAPFVKVNLGGISST
                     LFESEMFGHVRGAFTDARADRKGRFELADRGTIFLDEIGEIDPSAQVKLLRVLQDRSY
                     EVLGSSVTRTVDVRVVSATNRDLAEQVEKGTFREDLLYRLNLIAVRLPPLRERRGDIP
                     LLARHFLGLSARVYRREPREPLEITGRALEWLQEQSWPGNIRQLKQTLERAMLVLDGG
                     RLDVGDLRSLAELGSREVRGQGSALPSPGSMTLDEMEKAMIVQCMRHYDGNVTRVAEA
                     LGLSRAALYRRFEKYGIDPHGSGPHEG"
     gene            complement(18488..19684)
                     /locus_tag="VEL74_09590"
     CDS             complement(18488..19684)
                     /locus_tag="VEL74_09590"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYG62821.1"
                     /translation="MIRHLFKLVWNRKRANALLILEIFIAFLVLFVVATSSLYYWDSY
                     RRPLGFDSGNVWDISLMVNDSGFHWIPGQREGFSRLLEEIAALPQVEGAASVLLPPFG
                     LCAATTQGFRGRVLWSEYEEVMPGFDRVMGLHLVAGRWFRPGDEGLSWKPVVIDQDLA
                     RDAFAGKDPVGQVLFPPDPATREPEMRVIGVISEFRMGGELSGTGKNFFFKLRPEEPW
                     MRPPHHLLVKLRPGTPESFEKELYERLQRAAPGWSFQITPLAQLREAALRSRLGRLVL
                     GGTLAFFLLMMVALGLIGVLWQNLLQRTREFGLRRATGASGPQVRRQVLMEQLFLTGL
                     GLLLGTLLVVQLPLLGFAGFLSPEVFTAGLLTTLATMLFLTLACASYPSWRTSRIPPA
                     EALRYE"
     gene            complement(19681..20940)
                     /locus_tag="VEL74_09595"
     CDS             complement(19681..20940)
                     /locus_tag="VEL74_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG62822.1"
                     /translation="MLANYLKIAWKVLQRQKFYTFISLFGISLTLLILLVATAMFDSI
                     YGPQPPEVHSDRTLHLETLAMRGPKDVIFPGGPGYLFLDHFIRPMAAMPEVEGVAVIG
                     GQETAASYAGGQKALSTLRRTDGELWRILEFDFLEGRPFTVEEDRQGRFVAVINEATR
                     QRVFGRGPAVGRTFEIGGQTFRVVGVVRNVSSLRETSFADVWVPISTIKSDVYKRELV
                     GGFQALLLARSRSDFPAIRAELDRRLRQAKSHLPSYFDRLEGGADTLFESTSRYLPPG
                     GDPYERSRPGLLRAALFLSMLLFMLLPAINLVNINLSRILERSSEIGVRKAFGASSRA
                     LVGQFVVENLMLTLVGGAIGLALAAAVLHGLNASGLISYSQLSVNVRVFVYGLGIAVL
                     FGLLSGVYPAWKMSRLHPAEALRGRSR"
     gene            complement(20953..21618)
                     /locus_tag="VEL74_09600"
     CDS             complement(20953..21618)
                     /locus_tag="VEL74_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG62823.1"
                     /translation="MIHLQNVSKLYRAERIETLALQRINLQVSEGETVSIMGPSGCGK
                     STLLHIMGLLDEPSEGHVELDGRPVKRYADRDLARVRNEKVGFIFQSFHLIPDLKVVD
                     NVEIPLLYRRMPHGERRSMALAALERVGLTSRAHHFPAQLSGGQQQRVAIARAIVGRP
                     RLLLADEPTGNLDSQTGDEIIGILMGLNSGEGTTVVMVTHDLRLAERTGRVVRLFDGR
                     QVQ"
     gene            complement(21776..22171)
                     /locus_tag="VEL74_09605"
     CDS             complement(21776..22171)
                     /locus_tag="VEL74_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007660314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYG62824.1"
                     /translation="MNRFFLSPHADRIYALLRIVAGFMFLCHGLQKLFGVLGGQQQPI
                     ASLAGVAGVIEFVAGVLIMVGFLTHWAAFLASGTMAVAYFMVHQPQGTLPIQNQGELA
                     ALYCFVFLYIAARGSGPWSVDASMRGSRR"
     gene            complement(22513..23130)
                     /locus_tag="VEL74_09610"
     CDS             complement(22513..23130)
                     /locus_tag="VEL74_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62825.1"
                     /translation="MSHPLSKLALASLLILPLGTVGCSKSQSPTEPAVDLESAVVSAE
                     SNTASVTEESRGRGRGGDDEAGDDRGRRRGRGSDDAAGDDRGRRGRRGRGGDDATTNN
                     GGGRRGGRNGGNGGNGEQRPRTGQEFEARVVSVDVGGGSLTLAGGATVRVNGQTQWSG
                     RGDLLSLTEIAGSVRSGDPTRVEGRGTRQGDGSILALTLKAEVDN"
     gene            complement(23226..>23406)
                     /locus_tag="VEL74_09615"
     CDS             complement(23226..>23406)
                     /locus_tag="VEL74_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62826.1"
                     /translation="GLTRARSRLFLFHSRKRTLRGQTMETRPSPFLADVEEALLERRD
                     EPVLPPRRPEQLSLL"
BASE COUNT         3383 a         8527 c         8121 g         3375 t
ORIGIN      
        1 ccgccgcgag gcccgccagc tcctcgccgc cgacctcgcc atcgaagggc gccgccccct
       61 gcccccggac ttcgacctcg cccgcgccgg cctcgccgac gtccggcgca ccgacatcac
      121 cgagctggtc accgtcgccg ccgcgccgcc gcgcggctcg gcccccggcc ccagccagct
      181 cgtggagctc aaggccatcc gcggggacta ccccttctac ggcgtgctcg agctgcggcc
      241 ggaccgcccc ctcgccgcgc tcctccgcgc cgacaccgtc gtggtcgcgg cggagctgct
      301 gtcgcgcctc ggcctgcgcg tgggcgacga gctgcgcatc ggcggccagc cgttccgcat
      361 cgccggcgtg gtggtctccg agcccgaccg ggtgagcgtc tcgctcaccc tgggcccccg
      421 cgtcttcatc tccgccgacg gcctcgcccg caccgccctg atccagcgcg gcagccgcgt
      481 cggctaccgc acgctcctca agctcccggc cggcttcccc ggcgccggcg tcgagcccgc
      541 cgccgagcgg ctgcgcctcg ccctgcccga caccaccttc tatgaggtgg agagctaccg
      601 cgccgcgcag ccggagctgc gcgacaacct ctcccgggtg gagcgctacc tcggcctggt
      661 ggccctgctc tccctcttcg tcggcggcat cggcgtggcg cagagcgtcc gcgcctggct
      721 cgccagccgc ctcgacgcca tcgccgtcct caagtgcctg ggcctgcgcc cgcgcgagat
      781 cttccccctc tacctcggcc agaccgccct gctcggcctc gccggcagcg tggtagggat
      841 cgtggccggt gccgcggtgc agctcgccct cccctcgcta ttcccggacc tgatcccggc
      901 ggagctgatc cgcccctggc agccggcgtc gctgctgcgc ggcctcggcc tggggctggg
      961 ggtggcgctg ctgttcagcc tgccgccgct ctccgcggtg ctgcgcgtgc cgccggcgcg
     1021 ggtgctgcgc cgggacgccg agccgctccc ccgccaccgc tgggcggcgg cggtcaccgg
     1081 cgtcgccctt gtcgccggcg tctggggcat ggcgaccctc cagtcccgct cgtgggacct
     1141 gggcgcccgc ttcaccggcg gcgtcgccct ggtgacggtg gtgctcgccg ccgcggcgct
     1201 cctggtatcg cgcggcgcgc agcgcatccc ccgcgacctc tccccctcct gggcccgcct
     1261 ctggctgcgc cacggcctcg ccgccctggc ccgccccggc gccggcacgg tgggggcgat
     1321 cgtggccatg ggcctcggcg tgctggtggt gctcggcatg tcgctggtcg agcgccggct
     1381 ctcccgcgag ctgtcgaccg ccatcccgga gaacgccccc agcgccttcc tcgtcgacat
     1441 ccagccggcg cagtggccgg gcatcgaggg ggtgctgcgg caggccggcg ccacccgcat
     1501 cgattcggtg gcggtggtga tggcccgcct gagcgccatc gacggcaagg ggatcgagga
     1561 cctggccgcc ccggaacccg ctccggccga ggaggccggg acgcagacga acaaccgtcc
     1621 tgacgagcgg gcccgccagg gacgggggcg gcgccgcggg ggcgaggacg gcgcgcgccg
     1681 ctgggccctc acccgcgagc agcgcctcac ctatatgaag gagctgccgg ccgacaacga
     1741 gatcgtcgcg ggggcgctgt ggagcgaccc cgccagggcc gaggtgagcg tcgagaagga
     1801 ctatgcggac gacctggacc tgaagctcgg ctcggtgctc cgcttcgaca tccagggggt
     1861 gccggtcgag gtgacggtca ccagcctgcg caccgtcgac tgggggacct tcggcatcaa
     1921 cttcttcctg gtggtcgagc ccggggtgct ggaggaggcg ccgcagcagc gcctgaccgc
     1981 cgtgctcctc ccccgcggcg cggagcagcg ggtgcaggac gagatcgccg ccaactaccc
     2041 caacgtcacc atgctgcgca tccgcgagat cctggagaag gtcctcgggg tgatgaagcg
     2101 gatcggcctc gccatccgtt tcctgggcgc cttcacggtg ctcgccggca tcgccatcct
     2161 ggccggcgcg atctccgccg gctcggcgcg ccgcggccgc gaggtggcgc tcttgaagac
     2221 cctggggatg actcggcgcg gcgtggccgc caccttcgcg gtggagtacg ccctgatcgg
     2281 cctggtggcg ggggtcatcg gcgccctcgg cgccgcggtg ctcgcccggg gcgtcgtcac
     2341 ccagggcttc gagctcacct ggcaggccga gccgggcccg gtcactctcg ccatcctggc
     2401 gagcgtcgtc ctgacggtgg cggccggcct cgccgccagc ttccgggcgc tggagcggcg
     2461 gcccatcgag gtcttgcgga gcgagtaggg aacttgcagg ggtggagatg gcatggacgg
     2521 gctcacgtct cgcaccatcc ccaccaacgg catccacctc cacgccgtgg aggccggccc
     2581 ggcggacggc ccgctggtcg tcttcctcca cggctacccc gaattctggt acgcctggcg
     2641 taagcagatc gcgcccttcg ccgctgccgg cttccgcgtc ctcgcccccg acctgcgggg
     2701 ctacaacctg agtgacaagc ccaaggggat ctcctcctac cggatcgacc acgtggcgcg
     2761 cgacgtcctc gggctgatcg acgaggccgg ccgcgacaag gcgttcatcg ccgggcacga
     2821 ttggggaggg gtggcggcct ggtggctcgg catggcgcat ccggaccgcg tcgagaagct
     2881 ggggctcctc aacatccccc atcccgcggt ggcgcggcag cacatcctga agaacgggaa
     2941 gcagcggcgc cggagctggt acatcttctt cttccagctc ccctggctcc ccgagcgggc
     3001 cttcaaggcc aggaacttca ccttcgccgc caagtcgctg cggggcagcg cccgccgtgg
     3061 caccttcacg gacgaggacc tcgcccgcta ccgtgaggcc tggcgccagc ccggggcggc
     3121 gcgctcgatg ctgaactggt accgcgccgc gccatgggcg ccgaccgcca agctgccgag
     3181 ccagcgggtg acggtgccca ccctcctcct ctggggagcg aaggacatcg ccctgggccg
     3241 cgagctcgcc cagcccagca tcgaccgctg cgacgacggc cgcctcgtct tcatcgagga
     3301 ggccgggcat ttcgtccagc acgaggagcc ggagcgggtg aacgggatgc tgggggattt
     3361 cttctcgtag aggctgaagc tcccggcggg gtatcagagt ctccgacggg ccgttgcaag
     3421 cttcgacggc ctgccgaagc tcccgacgcg ctgtcagagt ttccgacgga ccgttgcaag
     3481 tttcgacggc ttgccggagt gttcgacgcg ccgttggaag cttccgacgc gctgtcgaag
     3541 cttccgacgc gccgtcagag tttccagcgc gccgccgaag tctccgacgg gctgtcgtac
     3601 tctccgacgc gccagcgagc tctccgacga gtcgtcggaa gcttcgacgg gccgacggaa
     3661 gctccggcgc gccgccggaa gctctgagaa gccgtcggaa acttcgccaa gaaaaacccc
     3721 cctccttgac ctcccccact ccctaccgcc cacccgccca gggggggagg caccgccgcc
     3781 ccctgctccg gccgtccgtg atggtccgtg agcgtctgtg ttcgtccgtg tgtccgtgtg
     3841 tccccgcccg ggatcgtttt cggctcctcg tgtgtctaac ggagccagga gggtcgatca
     3901 tgcgcaagcc gctggcaaat tccggtcccg tctgtcccct tctgtcccca tctgtccccc
     3961 gaaggggcag ccgggcaccg gtttcttcag ggagcccaga aaaactgtgg ctccctttgc
     4021 atggaggtgt gagatgcgca agaagatgct gttgctggcc ctcgccctga ccgccgccgc
     4081 gtcgctttcc atccagcgcg ccgaggcggc cggtggctat agctgccccc agtgcgtgac
     4141 gtacgccgac ggctcccggt gctgcgtgag ctgctggtgc aactcgtccg gattccccat
     4201 cgcctgtacc gacaactact gtccgcctcc gggcggcatc aactaggacg gaggaggcgg
     4261 gcgaccagcc cgctcccggc gaagccggcg ggccctcccg ccggccgttt gctgttagca
     4321 tagtcgtggt ggtacgagca gccggaccgc ctccggccct cgttccttct cctgttcggg
     4381 agtgatgggg atcctccagt acgccttacc aattcttcgc cggggccttc cgcgcccggt
     4441 ggggccaccg ccgggtcgcc caggcccgtg gcccctccct catagattca cccatgacga
     4501 tctgtaacgc cgcaacgggg tgggacgccc aaccagggcg agtggcgggg agagccccga
     4561 ttccatgcat gcatgcatgc aaaaaggagg atgtctgatg agcgagcagg agatcaggtc
     4621 caggatcaag cagatcatct cgaacatcgc cgggctcgat ccccagagga tcggcgacga
     4681 cgactccttc accggtgacc tcagcctcga ctcgctgtcc atgctggaga tcggcgtcga
     4741 cgtcgacatg gcgttccagc tcggcctgcc ggacgagcgc tacaaggagg tccggacgct
     4801 gccgcagatg gtggcgctgg tgcaacaacg acgaagcgag atcggcggcg gcgtcccggc
     4861 cggggccctc caggaggccg tctcctgatg cacggccgca tcgcggtcac cggcctgggg
     4921 tgggtcaccc cgatcggcgc cggccgcgat gaggtctggg aggggttgct cgccgggcgc
     4981 agcggtttcg ctcccgtcga gtcgttcgat acggccaggt tcagcgtcca cgtcggagcc
     5041 gaggtgcgcg gcttcgacgc ccggccctgg atccgccgcc tcgaccccgg ccgcctcggc
     5101 cgcgcctccc agctcgccat cgccgccgcc cgcatggcgc tcgaggacgc gggcctcgcg
     5161 gacctcgacg cggagagcca ccagggccgg gagcggaccg gcgtggtcat gggcaccacc
     5221 tccggcgagc cgcgcgaggt ggagcgcttc gacgaccgct acctcgccgg cgacacggcg
     5281 gcgatcggcg ccgagttcat ggggctctac ccgtgccaca tgatcgccgc ccacatcgcc
     5341 cgcgagatgg ggatccgcgg cccggtcgag atgatcccca ccgcctgcgc cgccggcaac
     5401 tacgccatcg cccgtgcctt cgacctcctg cgcaccggcc gctccgacct ggtgctggcc
     5461 ggcggcgccg acgccttctc ccgcatcacc tatgccggct tccaccgtct gggcgccatc
     5521 gcccccgagc gctgccagcc gttcgaccgc gaccgcaagg ggatgatccc cggcgagggc
     5581 gcggcggtcc tcgtgctgga gccgctggag cgggcgcggg cgcgcggcgc gcgggtctac
     5641 gccgaggtgg ccggctacgg gctctcctgc gacgcccacc acatgacggc cgctcacccg
     5701 gaaggggacg gcgcggcgcg ggcgatgctc gccgcgctgg ccgacgccgg cctcgaggcg
     5761 cgcgacgtct cctacatcag cgcccacggc accggcaccc cgaccaacga ccggctggag
     5821 accctggcgg tgcagcgggt gttcggcgag gcggcggcgc ggacccccat gagctccatc
     5881 aagtcgatga tcggccacac gatgggggcc gcctcggcga tcgaggccgg ggtctgctcc
     5941 ctggcggtgg cgaccggccg cgtccccccc accatgaaca tggaggcgcc cgacggcgac
     6001 ctcgactacg tccccaacgc cgcccgcgag ctcgacgtgc gggtggcgat gaacaacgct
     6061 tatgccttcg ggggcaacaa cgcctccgtc ctcttcaggt ctacggagca ctgacgtgcg
     6121 gcgggtcgtg atcaccggcg ccggcgtcct gtcgcccctg gccgactccc ccgccgggct
     6181 ccacgcggcc ctgtgcgccg ggcagtccgc cctgaaaccg gtggagctct tcccgctgga
     6241 cggcgtcacc ccccgtccgg cgggcgagat ccgccccttc gatcccaagg cctacctcgg
     6301 cgagcgcaac ctgcggccgg tggaccgcac ctcgcggctg ctcctggtgg cggcccagca
     6361 ggcgctcgag tccgccggct ggaccgccgg ggagcgggcg gcgcgggagg tcgggctggt
     6421 cctggggacc accttctgca gcgtccacac gatcgccgag ttcgaccgcc gcgccgtgca
     6481 gctcggcccc tcctacgcga gccccatgga cttcgccaac agcgtcatca acgccgccgc
     6541 gggccaggcc gccatctggc acggcctgcg cggcgtcaac tccacggtat cggcgggcga
     6601 ggcgtcgggg ctcctcgccc tggcccatgc cgccgacctg atccgcgccg gccgggcgga
     6661 agcgctgctc gccggcggcg ccgaggagct ctgcttcgag tcgttcctgg gctgcgagcg
     6721 cgccggccgc ctggggctct cggtgaccgt ccccttcgac gcccggcgcg acggcttcgc
     6781 gctggccgag ggcgccgccc tcctcatgct ggaggaggcc gggtcggccg cggcccgcgg
     6841 cgcccgcgtc ctcgccgagg tgcgcgggca cggcaaggcg ttcaacccgg cgggcgccga
     6901 ggacgaggcg agcgccgtct ccgccgtcgc ccgcgccgtc cacctggcgc tggaggacgc
     6961 ggcggtcggt cccacggacc tcgacgtcct gagcctctcg gccaacggca gcgtccatgg
     7021 cgaccacaac gaggcctggg gcgtcggcat ggccctggat ggagcgggcg acaggcccgt
     7081 agccgccatc aaggcgatgc tcggcgaggc gctgggcgcc tccggcgcct tccaggccgc
     7141 ggccctcctg ggcaccttcg gcgacggcgt ggtccccggc gtcctggggc tggagcggac
     7201 cgagggcggg ttcccgctcg ccggcgtccg ggcagagacg cggcgcctcg gccgcgcgcc
     7261 gcggctcggg ctgctccacg ccatggcctg ggacggccag agctgtgccc tggtcctcga
     7321 ggcacccccc gaggcgaggc catgaacggt ccggagcggc ccgggatcgt cccccgcttc
     7381 gaggagcagg gctactcggc cgaggccatc gcggagcgcc gccgctggct gaccgagcgg
     7441 accggcgccc ccctcaccca cgtcggcgcc ttcaccgtcc ccggcgagga gatgcggggc
     7501 aacatcgaga acccggtggg cgccgtccag gtgccgctgg gcgtcgccgg gccgctcctc
     7561 gtccagggcg agcacgcgcg ggggaccttt tacgtgccgc tcgccaccac cgagggggcg
     7621 ctggtgcgct cctacgagcg cggggccgcc gccatcaccc gcgccggcgg cgccaccgtc
     7681 cgcctctacg ccgacgagaa ccgcgtctcc ccggtcttcc tgttcgacag cgtggccgag
     7741 gccgccgact tcgtccgcgg cctgcccggc tggttcgagg ccgtgcgcgc cgcggccgag
     7801 tccaccaccc ggcacggcaa gctgctgcgc ctggagcccc ggccgctcgg ccgcgaggtg
     7861 gtggtccatt tctgctactc gaccggcgac gcgcacggca tgaacatgat cgtccgcgcc
     7921 accgaccacg cctgccggtg gctgctggcg aacaccccgg cgcggcggca cctcgtcttc
     7981 agcggcctgg aggccgagaa gcgggcggcc ggctcgctct tcgccggcgg caaggggaag
     8041 aaggtggtcg ccggcgccct ggtgccggac ggcatctgcc gcgcctggct ccacgccacc
     8101 cccgggcagt tgacggaggt ctggcgccat accctcctcg gccacgtcca aggggggacg
     8161 ctgggctaca acggccactt cgccaacggc ctgaccgcgc tgttcatcgc ctgcggccag
     8221 gacgtcgcca acgtcaccaa ctccgccgtg ggcgtcacct ccttcgaggt caccggcggc
     8281 ggggacctct acgccagcgt caccctgccg tcgctcaccg tcgccacggt cggcggcggc
     8341 accggcctgg ggaccgcccg cgagtgcctg gaggtgctgg ggtgcgcggg agatgccgcc
     8401 cgggggggcg cgccgaagct ggccgagatc gccgccgccc tgctcctggg gggcgagctg
     8461 tccatggccg gcgccatcgc cagcggcgag ttcgtccagg cgcacgaagc ctacggccgc
     8521 aaccggccgg ccgcccccgt ggaggccggc gcttgaaacg gctgttccgc ggcctgcggc
     8581 gaccgccgga cgccacctac gacgccgtgg tcatcggcgc cggcatcggc ggtctggtca
     8641 ccgccaacct gctggcgcgg gaggggctcc gggtcctcct tgtcgagcag cactacatgg
     8701 tggggggcta ctgcagcacc ttcaagcggc gcggctacgt cttcgacgcc gccacccact
     8761 tctatccgct gctgggcaat gcggagacgc tgaccggccg cctcctcgcc gagctggggg
     8821 ccaccaccgg ctgggtgaag atggacccgg tcgacacctt ccatttcccg gacggctcgc
     8881 gcttcgaggt gccggccgac ttcgacgcct acctggcgcg cctcaaggcc gagttcccgg
     8941 aggaggccgc cgccctcgac tccttcttcc aggcggtccg cgagacctac ctgctcggcc
     9001 tcctccatta cttcagagga cggccgctct cccagctcgg ccggctggcg ccgtaccgcg
     9061 acctgaccgt ccgcgaggcg ctggaccgct ggttccgcga tcccaagctg aagctgctgc
     9121 tcaccgccga ctgcccgcac tgggggtcgc cgccgggccg cacctccttc gtcttcgact
     9181 ccatgctgcg gctctcctac ttcctcggca actactaccc gcaggggggc tcccaggcct
     9241 tcgccgacga gctcgcgcgc tgcttcgagg agcgcggggg gcacgtcctc accagcgcga
     9301 tggcgcaacg catcctgatc gccggcggca cggcgtgcgg ggtcgagctc gagatcctcc
     9361 gcggcccgct cgccggctcg tcgttcaccg tggccgccgg cgccgtggtc tccaacgccg
     9421 acctgctcct caccctggag cggctgatcg gcgccgcgca cgtggcgccg gagacactcg
     9481 ccgggctgcg gtcgctgcgg ccgacctatc cctgcttcct gacctacata gggacggccg
     9541 gcatccccga ctcggtcctc gaggacgtcc agggctacta ctgggacggc tgggacatgg
     9601 accgggtggg caccgacgcg ttgcgcttca agatcttcgc cccgaccctc tacgagccgg
     9661 gcatggcgcc gcccgggggg caggtgatcc tggtgcagaa ggtcctggag atggactacg
     9721 gcgccgtcac cgactggccg gcgcacaagc gtcaggtgga ggagcaggcc ttccagcacc
     9781 tggagcggct gatccccggc ttctccgggc acgtggtggt caccgacagc tcgtcggcgc
     9841 gcagcgcctg gcggttcacc ctcaaccatc agggggccat gctgggctgg gagatgtccc
     9901 cctggcagct cggcgaaggg cggcccgaca tcgccggaga gatccggaac ctctacttca
     9961 ccggccactg ggtccgcccc ggcggcggca tcaccccggt catcgtctcg gcgcagcagg
    10021 ccgcggcggc cgtgctgcgg ggagctggag acgtggatac aagggaggag ctatgcagca
    10081 cgctaatgac tgcgacgtcc tgattctcgg cgccggcctc gccggcgtct gcctcgcccg
    10141 ccagatcctg ctcgccgatc cggagaagcg gatcatcatg gtggaccgcc ggagcgacct
    10201 gccgcccacc aagcagaagg tgggggaggc cacggtccag atgagcggct actactacgc
    10261 caaggtcctg gagctcgagg agtacctgct ccaggagcac tacctcaagt acaacctgcg
    10321 cttcttctgg ccgtcggcgc agggggcgga ccgctacgaa gagctcagcc agtcctacat
    10381 ccgcggcctc tccaacatcc cgacctacca gctcgaccgc aacaagttcg agggcgaggt
    10441 gctgcggcgc aacctggccg acccgcgttt caccctctac gcgccggtga agaacatcga
    10501 gatcgacctc gccgagaccg tcgcggccgg cccccactcc ttcggtttcg agaccgggga
    10561 cgggaaggtt tcgggccagg ccggctgggt ggtggacgcc accggccgcg gccggctgct
    10621 gtccaagcgc cggcagctcc agcggccgag cccgatccat cacggcacca ccttcttctg
    10681 ggtggacggc ctggtggata tcgagcggct gaccgacctg acgccgaagg aggtgcggct
    10741 gcgccccgac cgctccaccc tcgggcatac gcccgccttc ctggccacca accacttctg
    10801 cggcgagggg tggtggttct gggtcatccc cctccacaac cgcaccagcc tggggctggt
    10861 ctacgacaac cggctggtgc cgcgggaaga ggtcgcgacg gcggagaaga cgatcgattt
    10921 cgtctgccgg cactaccccc tcttcgagcg cgccctgagg aaccgcgaga tcatcgacgc
    10981 gagcggcttc gtcagcttcg cgcacgactg cggccagacc ctgagcaccg cccgctgggc
    11041 catggtcggc gaggcctgcc gcttcacgga cccgctctac agccccggcg gcgacctgat
    11101 ctccacctac aacacgctga tcaccgacgc catcctcacc gacgacccgc gggagctgga
    11161 gagcaaggtc cgcctctacg agggcctggc gcgagccgtt tacgaatcct acgtgccgag
    11221 cttcgcggtg agctacagca ccctcggcga ccaggagtgc ttctccctgc gctacacctg
    11281 ggagctcgcc atctacttct ccttcttcgt cttcccgttc atcaacgacc tcttcaccac
    11341 ccgcgccttc gtgcccggct tcctgcagcg cttcggccgg ctcgggccga tgaaccgggg
    11401 ggtgcacgag ctcctcgccg gctactatga atggaagaag gagcacccgc gcggcccggc
    11461 acgcgagccc ctgttcttcg atttctacga gctctggccg ctcaaggcgg cggagacctg
    11521 cttctaccgc gtcggcgtca cccccgagga ggcgcgccag atcctcgacg agcatctcgg
    11581 caacctgcgg gagaacgcgc gctggatcta tgcgtacgtc gccgccacgg tcctgggaga
    11641 cgagcgggcg gtgacacatc cagagttcgt cggcggcatc gacgtggaca acctcgcctt
    11701 cgatccccgg gagatgcggg agcgtctgga gcgctgcggc gagaccgatg agcgctggag
    11761 ctggaacttc gagccgccgg cgctctgccg gttccgcaag gaggtggcgg ccgtggagcc
    11821 catggaggag gccgaggagc cgatgatggc cgccggaggc gcccggtgat caccgcgcag
    11881 cgcccggtgc tggacccgcc gcccgatctg gcgccgggcc tggagcgggc gttcgccttc
    11941 gtgccggagg agcgctccgg ccctgtccag gtggagggga gcctccccgc ctccctgcgc
    12001 ggcacctact acctgaacgg ccccgcccgc ttcgcccgcg ccggcctgcg ctatcgccac
    12061 tggctggacg gcgacggcat ggtctgcgcc ctgcgcttcg atgacgccgg ggaaagcggc
    12121 gcccacctca cggcccgctt cgtgcgcagc gccaagtgga cggccgagga ggaggccggc
    12181 cgccccctgt tccgcgcctt cggcaccgcc tttcccggcg accggctggt gcggggggtg
    12241 gcgctggagt cgccggtcaa cgtcagcgtc gtcccctggc gggacaccct gctggccttc
    12301 ggcgagcagg ggctccccta cgagctcgac ccggtgacgc tcgagacccg cggcctctac
    12361 aacttcggcg gcacgctcaa cccggtgagc ccgctcgccg cccacgccaa ggtcgatccc
    12421 accaacggcg agctgctgga cttcggcgtc tccttcgcca gcgcccggcc gctcctcaac
    12481 ctctatcgct attcggccga gggggccctc ctctatcgcc ggcggctccc cctccccttc
    12541 ccctgctccc tgcacgactt cgccctcagc ccgcgccacg ccgtcttcta tctcgccccc
    12601 tacgtgctcg acgtcgagcg gctgctgcgc gagggctcca ccctgatgga gtcgctggtc
    12661 tggcggcccg agctcgggac ccgcctcctg gtcgtggacc ggcagaccgg cgaggccgtg
    12721 gccgatctcc cgatcgggga ccgctactgc ctgcacacca tcaacgcctt cgaggagggg
    12781 gacctcctgg cggtcgacgt ggtggagctg gaccgtccgg tctacgacca gtacgaggtg
    12841 gtgccggacc tcttcacctc cgtctccccc ggccgccccg tgcgcctgct ggtggacctg
    12901 agagggggga gcctcgtgga acgccgggag ctgggcccca ccgaggcgcc ggacttcccg
    12961 gcgatcgccc cctgccgcac gggcctgccc accgaggacg tctggatgct ggacatctcc
    13021 accgccggcc gccccggccg caaattcttc gaccggctgg tccaccttcc ggccgggggg
    13081 acgccggacg tctggcaggc gccgcccggc gtctatctgg gaggagagcc cgccttcgcg
    13141 ccgctcgccg gcgaggagcg gggcgccgtc ctctgccagc gcttcgacgc ggtggcgaac
    13201 gagagcgcgt tcctggtctt cgacgccttc ggcgtggccg ccggcccgcg cgccgtgctg
    13261 ccgctcgagt ccccgcttca cctgggtttt cacgccgtct tcgttccgga atgatcccca
    13321 tccgagagga gagcgtcatg cccgatacca cccctgtcct ctaccccgag accatccacg
    13381 atcccgcggc cgtccgcagc cggaccgtca cctggcagga ccccgcgctc ggcctctccc
    13441 gggcgcgcga gatgtccggg ctcgactggc tgaacgagat gttccgcggc aagctgccgc
    13501 gcccgccgct caacaacctg ctgggcgtcg acgaaggggg cgccgagccg ggccgcgcct
    13561 ggatgagctt cccggcgggc gagcacctgt acaaccccat gggcacggtg cacggcggag
    13621 tgctgtccac ggtgctcgac tcggcgatgg gctgcgcggt gcaatccacc ctcggcccgg
    13681 gcgagggcta caccacgatc gacctccacg tcaccttcgt gcgtccggtg accgcggcct
    13741 ccgggcggct gcgctgcgaa ggcgagatgg tccacgccgg ccggcgcctc gccatggcgc
    13801 aggcgcgcct gaccgatgcc cagggacggc tctgcgccca cgcggtctct tcctgcatga
    13861 tccgccgggc cggcgaccac gacggacggg tctgagctgt gatctgatac ccgccatgca
    13921 cgactacgtc gcgtcgctgg tatccgaggt caaggccaag aaccccggag agcccgagtt
    13981 ccatcaggcg gtcgcggagg tcatggagtc gctggcgctg gtcctcggcc ggcggcccga
    14041 gtaccggcgc gccaagatcc tcgagcggct cgtcgaaccc gagcgcgtca tcctcttccg
    14101 ggtcccctgg caggacgacc gcggcgaggt ccacgtcaac cgcggcttcc gggtcgagat
    14161 gtgcagcgcc atcggcccct acaagggggg gctgcgcttc caccgctcgg tcaacctcgg
    14221 catcctgaag ttcctcgcct tcgagcagat cttcaagaat tcgctgacca ccctccccat
    14281 gggcggcggc aaggggggct cggatttcga ccccaagggc aagagcgacg acgaggtcat
    14341 gcgcttctgt cagagcttca tgtgcgagct gtaccggcac atcggcccga acaccgacgt
    14401 gccggcgggc gacatcggcg tcggcggccg cgagatcggc tacctcttcg gccagtacaa
    14461 gcggctgcgc aacgagttca ccggcgtgct caccggcaag gggctgcact ggggcggctc
    14521 gctgatccgc ccggaggcca ccggctacgg ctgcgtctat ttcgcccagg agatgctcgc
    14581 cacccgcggc cagacgctgg agggcaagac ctgcctggtc tcgggcagcg gcaacgtcgc
    14641 ccagtacacc gtcgaaaagc tcctcgacct gggcgcccgg ccggtgaccc tgtcggactc
    14701 cgacggcttc atccatgacg aggccggcct ggaccgcgac aagctcgcct tcgtcatgga
    14761 gctcaagaac gtccgccgcg gccgcatcca ggagtacgcc gagagatacg gcaccgccac
    14821 ctacgtgccg aacggccccg ggcccgccca ggggctcggc cacaaccccc tgtgggaccg
    14881 ccaggcggac tgcgccttcc cgagcgcgac gcagaacgag atcaacggcc gcgacgccgc
    14941 caacctcctg cgcaacggcg tcttcctggt ctccgagggg gccaacatgc cgtcggacat
    15001 cgacgcggtg aaccggtacc tcgacgccgg catcctctat ggaccgggca aggccgccaa
    15061 cgccggcggc gtcgccacct cggggctgga gatggcgcag aacagcatgc gctaccgctg
    15121 gacccgcgag gaggtggacg gcaagctgca gcagatcatg aaggagatcc accgcgcctg
    15181 cctggagacc gccgaggcct acggcgtgcc cggcaactac gtccacggcg ccaacatcgc
    15241 cggcttcctg aaggtcgccg aggcgatgat ggatcaggga ctggtctagt cctgcgggca
    15301 cgtccaggcg cggcgccccg gccactggcg ccacgccccg gacctgtgct tcgattgtca
    15361 aagatcccga ggagccgagt ctaaactaga cggatcactc cgctcctcga gcaggacaat
    15421 acatctatgg agatccaatt gcaacttcat gccgtacctc gtagactctg gccggagatg
    15481 ctgccgctga agccttgacg gcgactgggg agcgctccgg acggcctgga tgccgtccag
    15541 accgcccggg agctctccag atccgccggc agaggtccga gacgcacgga tggagtccgg
    15601 ggcgcccgga ttcactccac ggggctcggg atggcatccg gggcccctgg atcgtatcca
    15661 tggcgcctgg acggcatccg gagcgtccgg atggaatccg ggccgccgct ctttgacaac
    15721 tgaattcagg aggcctcggc ctcagaaccg gatccagagc tccgccccgc cagcgggggc
    15781 gttcctcagg ccgaaggcga agccgtggcg ggagaggatc tcgcgcacca gggtgaggcc
    15841 caggccgcgg ccgtcccgct tggtgctgaa gaagggggtg aagaggaggg gctccacgcc
    15901 ggcggggatg ccggggccgg tgtcgcggac ggcgagcacc gccccatccc cctcgcgttc
    15961 caggcgcagg gcgatccggc cgtcctcgcc gatcgactcc agggcgttct tgaacacgtt
    16021 gaccagcacc tgctccatct ggttgcggtc catggggatg ggcgctatcg gagccagggc
    16081 ctcggcccgc tcccaggcac aggcgacgcg ccgccgctcc agctccggcc gcagcaggag
    16141 gaggatctcg tcgaccagcc ggcggacgtc ggtggggcgc gggtccggct ccggcagccg
    16201 cacgacggtg gcgaagccgt tcatgaaggc gctcaggcgc tccatgcggg cgatggccac
    16261 gccgagggcg gcgaggaagt cgtcccggtc ctcgtcgccg agctggccgg catagccgcg
    16321 gaaggagtcg aggagcgacc ccacggcccc caccgagttg ttcacctcgt gcgacatcat
    16381 gcggatgagc ttgccgtacg ccgcgctctc cgaggcccgc agctcctccg tcagctcctc
    16441 caggaggaag aagctgcgcg ggaagccgcg gtcgaagaac tcggcccgcg aggccttgat
    16501 ccgccgcccc ccctggagcg gcagcacccg cgactccccc acccggagcg ccgccagcgc
    16561 ggcccccagc ggcccggcgc tcgcggccag cagccccccc tccgccgacg tctccagcaa
    16621 cgccgcggcg ctggggttcg cctgcgcgat ccggccgtcg aagtcgaggg tgaggatgcc
    16681 ggcgggcgag gcggccagga tccgctccag gaagaagtgc tgctcctgga gcttcaaccg
    16741 ctcctcccgc agccgggcca ccatctcgtt gtagacgcgg accagggcgt ccatctccgg
    16801 ctgccccacc tcccggaagg aagaggtgaa ctcccgttcg gacatcagct cggcgccggt
    16861 gcggatcagc tccaggggga cgaagaacga ccgcaccagc agcaccccca gggtggccga
    16921 cagcgcgaac agcgcctcca ccgccaggag cagcagccgg ttctcgcgca ccgcgaagac
    16981 cgcgatcgcc gccatgatcc cgtggatcag gacgaggtag aggatgacct tggcgcggag
    17041 ggacagggct tacccctcgt gcggaccaga gccgtgcgga tcgatgccgt acttctcgaa
    17101 ccgccggtag agggcggcgc gggagaggcc cagggcctcg gccacccggg tgacgttgcc
    17161 gtcatagtgg cgcatgcact ggacgatcat cgccttctcc atctcgtcga gcgtcatgct
    17221 gcccggcgag gggagggcgc tcccctggcc gcggacctca cggctgccga gctcggcgag
    17281 gctgcgcagg tcgccgacgt ccagccggcc gccgtcgagc accagcatcg cccgctcgag
    17341 ggtctgcttg agctgccgga tgttgcccgg ccagctctgc tcctgcagcc attcgagggc
    17401 gcggccggtg atctccaatg gctcccgggg ctcccgccgg tagacgcggg cggagaggcc
    17461 gaggaagtgg cgggcgagga gggggatgtc gccgcgccgc tcgcgcagcg ggggcaggcg
    17521 cacggcgatc aggttcaggc gatagaggag gtcctcgcgg aacgtcccct tctccacctg
    17581 ctcggcgagg tcgcggttgg tggccgagac cacccgcacg tcgaccgtcc gggtgacgct
    17641 cgaccccagc acctcatagg agcggtcctg gaggacgcgc agcagcttca cctgggcgct
    17701 ggggtcgatc tcgccgatct cgtcgaggaa gatggtgccg cggtcggcga gctcgaagcg
    17761 ccccttgcgg tccgcccggg cgtcggtgaa ggcgccgcgc acgtggccga acatctcgct
    17821 ctcgaacaga gtcgacgaga tccccccgag gttgaccttg acgaacgggg cgtcgcgccg
    17881 ccggctgttg cggtggatgg cctcggcgat cagctccttg ccggtgccgc tctcgccggt
    17941 gatcagcacc gaggcgtcgg tggggctcac ccggccgacg acctccagga gcttgaggaa
    18001 ccggggctcg ttgcccacca gcccccgaaa gtcgaagcgc cggtccagct cctcgcgcga
    18061 gggggtcctg ccctgacctt catcggcgcc ggagcgggag gcggcgagcg acagcgcggt
    18121 ctccaccgac tggaggagct gggcgttgct ccacggtttg gtgatgaagt cggcggcgcc
    18181 agccttcatc ccctcgaccg ccagggcgat ggagccccag ccagtgatca ggatcaccgg
    18241 caggtgcggg cgcaaccggc ggatctcggc cagcagctcc agcccctcct caccatcggt
    18301 gccgcgggag aagttcatat cctggagcac caggccgatc tcgtctggac cgggctcctg
    18361 cccacgcacg gccgccaggg cctcctcctg ggagcgcgcc gcccgggtcg agtagccgtg
    18421 gcgcttcagg aggagaccca gcgaggcggc caccgaagga tcgtcgtcga cgatcaggat
    18481 catggcgtca ctcgtagcgc agcgcctcgg ccggagggat gcggctggtc ctccagctcg
    18541 gataggaggc gcaggccagg gtcaggaaga gcatggtcgc gagggtggtc agcaggccgg
    18601 ccgtgaagac ctcggggctc aggaagccgg cgaagccgag gagcgggagc tggaccacca
    18661 acagcgtgcc gaggaggaga cccaggccgg tgaggaagag ctgctccatc aggacctgcc
    18721 tccggacctg ggggcccgac gcccccgtcg cccgccgcag gccgaactcc cgggtgcgct
    18781 gcagcagatt ctgccacagc accccgatca gccccagcgc caccatcatc aggaggaaga
    18841 aggcgagcgt gccgcccagc accagccggc cgaggcggga ccggagagcg gcctcccgca
    18901 actgcgccag cggcgtgatc tggaacgacc agccgggagc cgcccgctgg agccgctcgt
    18961 acagctcctt ctcgaaggac tccggcgtac ccggccgtag cttcaccaac aggtggtgcg
    19021 gcggccgcat ccacggctcc tcgggccgga gcttgaagaa gaagttcttg ccggtccccg
    19081 agagctcccc ccccatccgg aactccgaga tgacgccgat gacccgcatc tcgggctccc
    19141 gggtcgccgg atcgggagga aagagcacct gcccgaccgg gtctttgccc gcgaaggcgt
    19201 cccgcgccag atcctgatcg atgaccaccg gtttccagct caggccctcg tcgccgggcc
    19261 tgaaccagcg cccggccacc agatgcaggc ccatgacacg gtcgaagccc ggcatcacct
    19321 cctcgtactc cgaccagagg acccggccgc ggaagccctg agtcgtcgcg gcgcagaggc
    19381 cgaacggagg gagcaggaca ctggcggcgc cctccacctg gggcagcgcg gcgatctcct
    19441 cgagcagccg ggagaacccc tccctctgcc cggggatcca gtggaacccg gagtcgttca
    19501 ccatgagcga gatgtcccag acgttcccgg agtcgaaccc caacgggcgg cgatagctgt
    19561 cccaataata gagactggac gtcgccacca cgaagagcac caggaacgcg atgaagatct
    19621 cgagaatgag caaggcattc gcgcgcttgc ggttccagac cagcttgaac aggtgacgga
    19681 tcatcgtgac ctcccgcgca gggcttcggc cggatggagc ctggacatct tccaggccgg
    19741 atagaccccg gagagcagcc cgaacaggac ggcgatgccg aggccgtaga cgaacacgcg
    19801 gacattgacg gagagctggg agtaggagat gagaccgctg gcgttcagcc cgtggagcac
    19861 ggccgcggcc agggccaggc cgatcgcgcc gcccaccagg gtgagcatca ggttctccac
    19921 cacgaactgc cccaccagcg cgcgcgacga ggcgccgaac gccttgcgga cgccgatctc
    19981 cgacgatcgt tcgaggatcc ggctcaggtt gatgttcacc aggttgatcg cgggcagcag
    20041 catgaagagc agcatcgaga ggaagagagc ggcccgcagc aggcccggcc ggctccgctc
    20101 gtaggggtcg ccgccggggg ggaggtaccg ggaggtggac tcgaagaggg tatcggcgcc
    20161 gccctccaac cggtcgaagt agctgggcag gtggctcttc gcctgccgca gccggcggtc
    20221 gagctcggcc cggatggcgg ggaagtcgga gcggctacgg gcgaggagga gcgcctggaa
    20281 gccgccgacg agctcccgct tgtagacgtc ggacttgatg gtggagatgg gcacccagac
    20341 atcggcgaag gaggtctcgc gcaggctcga gacgttgcgc accaccccga ccacccggaa
    20401 cgtctggccg ccgatctcga acgtcctgcc cacggcgggc ccgcggccga agacccgctg
    20461 ccgggtggct tcgttgatca ccgccacgaa gcggccctgc cggtcttcct cgacggtgaa
    20521 gggcctcccc tcgaggaagt caaactccag gatgcgccag agctcgccgt cggtccgccg
    20581 cagagtcgac agcgccttct gtccgccggc gtaggaggcg gcggtctctt gcccgccgat
    20641 gaccgcgacc ccctcgacct cgggcattgc cgccatggga cggatgaagt ggtccaggaa
    20701 gagatagccc ggcccccccg ggaagatgac gtccttcggc cctcgcatgg ccagggtctc
    20761 gagatggagg gtgcggtcgg agtggacctc cggcggctgc gggccataga tggaatcgaa
    20821 catcgccgtg gcgaccagca ggatcaggag ggtcaggctg atgccgaaca ggctgatgaa
    20881 ggtgtagaac ttctggcgtt gcaggacctt ccaggcgatc ttgaggtaat tggcgagcat
    20941 ggcggcctcc cttcactgca cctggcggcc gtcgaagagg cgcaccaccc ggccggtgcg
    21001 ctcggccagg cgcaggtcgt gggtcaccat gaccacggtg gtcccctcgc cggagttgag
    21061 ccccatgagg atgccgatga tctcgtcgcc cgtctggctg tccaggttgc ccgtgggctc
    21121 gtcggcgagg aggagccggg gccggccgac gatggcgcgg gcgatcgcca cccgctgctg
    21181 ctggccgccg gagagctggg ccgggaagtg gtgcgcccgc gaggtcagcc ctacccgctc
    21241 cagggcggcg agagccatgc tccgccgctc gccatggggc atccggcgat agaggagcgg
    21301 gatctcgacg ttgtccacca ccttgaggtc tgggatcaga tggaaggatt gaaagatgaa
    21361 ccccaccttc tcgttgcgca cccgggcgag gtcgcggtcg gcgtagcgct tcacgggccg
    21421 gccgtcgagc tcgacgtgcc cctccgaggg ctcgtcgagg aggcccatga tgtggaggag
    21481 tgtgctcttg ccacagcccg agggccccat gatggagacg gtctccccct ctgacacctg
    21541 caggttgatt ctctggaggg cgagggtctc gatcctttcg gcccggtaca gcttgctgac
    21601 gttctgtagg tggatcattg gaatgcctcc gtattctcct gtccggagaa cctggagagc
    21661 acgcgccgtg ccaccagcta aaccgctctg gtggcaagac ttacgagaat cgagggggat
    21721 cggggaccgt ccgcggccgg acggggtgtc cggaagcgga cggtccgggg caggctcagc
    21781 gccgggagcc gcgcatcgag gcgtcgacgc tccaggggcc ggagccgcgg gcggcgatgt
    21841 agaggaagac gaagcagtag agggcggcga gctcaccctg gttctggatc ggcagcgtgc
    21901 cctgcggctg gtggaccatg aagtaggcca ccgccatggt gccgctcgcc aggaacgcgg
    21961 cccaatgggt gaggaacccg accatgatca agacgccggc gacgaattcg atcactccgg
    22021 cgacccccgc cagagaggcg atgggctgct gttgcccacc caggactccg aacagcttct
    22081 gcaggccatg acagaggaac atgaagccgg ccacgatgcg gagcagggcg tagatgcggt
    22141 cggcgtgggg ggagagaaag aacctgttca tgacacctcc ttggttgagg gttccgggga
    22201 ttgtacgccg cgcagcccgg ttcagggagg agattccttc gtcgcccccc gaggaagggc
    22261 cgtcattccc tgcgaaacgg gctccactcc ccgggaaacg atgaccattc gcggaggaat
    22321 gatcgccgtt tccttcgaaa tgatcgtcct tcctcaagga atggtggccg tttctcgaga
    22381 aattaaggcc acttcgccag gaatgaaggc cattccgcaa gaaatggcgg ccgttccccg
    22441 aaggacggcc accattcctg cgggaacgac agcggtttct gctggtatgg gggagggctt
    22501 tggcgccctg gttcagttgt cgacctcggc cttgagggtc agggcgagga tggagccgtc
    22561 gccctgacgg gtgccgcggc cctcgacgcg ggtcgggtcg ccgctccgca cggagcccgc
    22621 gatctcggtg agggagagca ggtcgccgcg gccgctccac tgggtctggc cgttgacgcg
    22681 gaccgtcgcg ccgccggcca gggtcaggct gccgccgccg acgtcgaccg agaccacgcg
    22741 cgcctcgaac tcctggcccg tccgggggcg ctgctcgccg ttgccaccgt tgccgccatt
    22801 gcggccaccc cggcggccgc cgccgttgtt ggtggtcgcg tcatcgccac cacggccgcg
    22861 ccggccacgc cggccgcggt cgtctccggc cgcgtcgtcg ctgccgcgcc cgcgccggcg
    22921 accccggtcg tcgccggcct cgtcatcgcc gccgcgccca cggccgcggc tctcctcggt
    22981 cacggaagcg gtgttggact cggcggagac cacggcggat tcgaggtcca cggcgggctc
    23041 ggtggggctc tggctcttcg agcagcccac ggtccccagc gggaggatca gcagggaggc
    23101 gagagcgagc ttggaaagag gatgagacat cgggtgctcc tttcgaatca gaggcgggac
    23161 cacgagaagg cccaccggtt tcgagaagtg agacagccgg aggtggcgaa tggaacaggg
    23221 gagtctcaca gcaggctgag ctgctccggc cgccgcgggg ggaggacggg ctcgtcgcgc
    23281 cgctcgagga gggcctcttc gacgtcggcg aggaaggggg aggggcgggt ctccatggtc
    23341 tgcccgcgca gggtccgctt gcggctgtgg aagaggaaga ggcgcgagcg ggcccgggtg
    23401 aggccg
//
LOCUS       DASPJI010000360         3350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_19644, whole genome shotgun sequence.
ACCESSION   DASPJI010000360 DASPJI010000000
VERSION     DASPJI010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19644"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3350)
                     /locus_tag="VEL74_09620"
     CDS             complement(<1..>3350)
                     /locus_tag="VEL74_09620"
                     /inference="COORDINATES: protein motif:HMM:NF019927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HYG62827.1"
                     /translation="PCRATVEALTGLDLAALHTHLSRGGRLDGAAPPEPRGHALQVPL
                     SARDPEAGFAPSPGVVDTLRVPAGPGLRADAAVEEGEVPAEPDLEIVRLTASGRSRSE
                     ALGRLQDGLARTVALVHGGTTDKPFLSELLDRSEVEDGTADPAWLARLVSGGEHLARR
                     GAEAALLDAAALTYQEEVDQARGRFFASAARGRPEVPGEIGGSVELRYRGRAYPFRVA
                     RLARDLYRVEPPGLRLEIALGPESRTGRRLTCGGRTWRVFATRQGRSLLIEVDGVPHR
                     VDRGAARVVRSPAPAVVTSLAVAAGDEVAAGAPLAVLEAMKMETTLVAPAAGRVARLL
                     VRRNVQVPLGAPILELEPASGAVSSGSAASEAPVRFDALPQEPGARTLGEVRRLMLGY
                     DYEPAAVERLVTGGVGGSQGADGDAAAVLAILRAFVDIGALFRRPGAREEEGRHSAEE
                     YLFTYLRDLDAGGEGLPPAFLDDLRRALAHYGVDVLGRSPRLEESLFRIAISHSRLAR
                     QVPPVLALLEGCLESGQPLGGDDLRGLLDRMIAESQGREPALYDLAREVRYRCFDRPI
                     LLASQERILAAAEKDLARLATSLPAGERSARIKALVEYPQPLHHRLSMRFAEAGPHLR
                     EAMLEVMVRRYYRIREIGDVRVRTVGDQSLAITDYDYQEERIRLLATHAEHERLASQA
                     RALRGLAEEARRDGRDVVIDLYARRADGPLADAEAAAEVADLLASPGFPRGLQRVAVA
                     LSSPAGVQHFTFRRTDNGGYAEDAVYRGIHPMMARRMELWRLSNFHLQRLPAPEGVYL
                     FHGRAQENPGDERLFALAEVRELTVRRDEAARDGAGRVGLPQLEHTLMEALAGMRRFQ
                     ARRAAGDRLQWNRVLLNLWPVIDLEMEELRGMVERLSPLTEGLGIEKVAIRCRIPDRE
                     TREPRAWVLEISNFAEGGLVLRFRKPSDVPLKPLREYAQKVIELRRRGLTYPYELVKR
                     LAPSRDGSQGDLPPGDFAEHDLDAEGRLVPVHRPYGQNRANVVVGVVRSFTGRYPEGI
                     TRVIVLGDPSRGMGSLAEPECRRIVAAIELAGRMGVSLEWFAVSAGARISMESGTENM
                     DWIGLVLRRLVEFTQAGGEVNVVVLGIN"
BASE COUNT          434 a         1219 c         1300 g          397 t
ORIGIN      
        1 cgttgatgcc gaggacgacg acgttcacct cgccgccggc ctgggtgaat tccaccagcc
       61 gccgcagcac caggccgatc cagtccatgt tctcggtgcc gctctccatg gagatcctgg
      121 cgccggcgga gacggcgaac cactccaggg agacgcccat ccggccggcg agctcgatgg
      181 cggccacgat gcgccggcac tcgggctcgg cgagggagcc catgccgcgg ctcggatcgc
      241 ccagcacgat gacgcgggtg atcccctcgg gataacggcc ggtgaagctg cgcaccaccc
      301 ccaccacgac gttggcccgg ttctggccgt aggggcggtg gaccgggacg agacgcccct
      361 cggcgtcgag gtcgtgctcg gcgaagtcgc cgggcggcag gtcgccctgc gagccgtcgc
      421 gcgagggggc caggcgcttg accagctcat agggataggt gagcccccgc cggcgcagct
      481 cgatgacctt ctgggcgtac tcccgcaagg gcttgagcgg cacgtccgac ggtttccgga
      541 agcgcagcac cagcccgcct tcggcgaagt tggagatctc cagcacccag gcccggggct
      601 cgcgggtctc gcggtccggg atgcggcagc ggatggctac cttctcgatc cccaggccct
      661 cggtcagggg ggagaggcgc tcgaccatgc cgcgcagctc ctccatctcc aggtcgatca
      721 ccggccagag gttgaggagc acgcggttcc actgcagccg gtcgcccgcg gcgcggcggg
      781 cctggaagcg gcgcatgccg gcgagcgcct ccatcagcgt gtgctcgagc tgggggagcc
      841 cgacgcggcc ggcgccgtcc cgagccgcct cgtcgcgccg gacggtcagc tcccgcacct
      901 cggcgagggc gaagagccgc tcgtccccgg ggttctcctg cgcccggccg tggaacaggt
      961 agaccccttc gggggccggc aggcgctgga ggtggaagtt ggagaggcgc cagagctcca
     1021 tgcgccgggc catcatcgga tggatccccc ggtacacggc gtcctcggcg tagccgccgt
     1081 tgtccgtgcg ccggaaggtg aagtgctgga cgcccgcggg gctcgacagc gcgaccgcca
     1141 cgcgctggag cccccgcggg aagccggggc tcgccagcag gtcggcgacc tcggccgccg
     1201 cttcggcgtc ggccaagggc ccgtccgccc gccgggcata gaggtcgatg accacgtcgc
     1261 ggccgtcgcg ccgggcctcc tcggcgaggc cgcgcagggc ccgcgcctgg gaggccagcc
     1321 gctcgtgctc ggcgtgggtc gccagcaggc ggatgcgctc ctcctgatag tcgtagtcgg
     1381 tgatggccag gctctggtcg cccacggtcc gcacacggac gtcgccgatc tcgcggatgc
     1441 ggtagtagcg ccgcaccatg acctcgagca tcgcctcgcg caggtgcggc ccggcctcgg
     1501 cgaagcgcat ggagaggcga tggtggagag gctgcgggta ctcgacgagc gctttgatgc
     1561 gggcggatcg ctcgccggcc ggcaggctgg tggcgagccg ggccaggtcc ttctcggccg
     1621 cggcgaggat gcgctcctgg gacgcgagga ggatcggccg gtcgaagcag cggtagcgca
     1681 cctcccgcgc caggtcgtag agcgccggct cgcgcccctg gctctcggcg atcatccggt
     1741 ccaggaggcc gcgcaggtcg tcgcccccga gcggctggcc gctctcgagg cacccttcga
     1801 gcagcgcgag aaccggcggc acctgacgcg ccagccggga gtgggagatc gcgatgcgga
     1861 acaggctctc ctcgagccgc ggcgagcggc cgaggacgtc gacgccgtag tgggcgaggg
     1921 cgcggcgcag gtcgtccagg aaggccgggg ggagcccctc gccgccggcg tccaggtcgc
     1981 gcaggtaggt gaacaggtac tcctcggcgc tgtgtcggcc ctcctcctcg cgggcgcccg
     2041 gccgccggaa gagggcgccg atgtcgacga aggcgcgcag gatggcgagc acggccgcgg
     2101 cgtcgccgtc cgccccctgg cttccgccga cccctcccgt gacgagacgc tcgacggcgg
     2161 caggctcgta gtcgtagccc agcatcagcc gccggacctc gcccagggtc cgggcgccgg
     2221 gctcctgcgg cagggcgtcg aagcgcaccg gcgcctcgct ggcggcagac cccgaggaga
     2281 cggcgccgga agcgggctcg agctccagga tcggcgcgcc cagcggaacc tggacattgc
     2341 ggcgcaccag cagccgggcc acccggccgg cagccggcgc caccagcgtg gtctccatct
     2401 tcatggcctc gagcacggcc agcggcgcgc ccgccgccac ctcgtccccc gcggccacgg
     2461 ccagtgaggt caccacggcg ggggccggcg agcgcaccac ccgggcggca ccgcggtcga
     2521 cgcgatgggg cacgccgtcg acttcgatca gcaagctcct gccctggcgt gtggcgaaca
     2581 cccgccaagt ccggccgccg caggtgagcc ggcggccggt ccggctctcg gggcccagcg
     2641 cgatctcgag gcggagcccc gggggctcga cgcggtagag gtcccgggcc agccgggcga
     2701 cgcggaacgg gtaggcccgg ccgcggtagc gtagctcgac ggagccgccg atctcgcccg
     2761 gtacctccgg gcggccgcgc gccgccgagg cgaagaagcg gccgcgggcc tggtccacct
     2821 cctcctgata ggtgagggcg gcggcgtcga gcagcgccgc ctcggcgccg cgccgggcga
     2881 ggtgctcgcc gccggacacc agccgcgcga gccaggccgg gtcggcggtg ccgtcctcga
     2941 cctcggagcg gtccagcagc tcgctgagga agggcttgtc ggtggtgccg ccgtggacca
     3001 gggccacggt gcgggcgagc ccgtcctgca gccggcccag ggcttcgctc cggctccggc
     3061 cgctggcggt caggcggacg atctccaggt ccggctccgc aggaacctcc ccttcctcca
     3121 ccgccgcgtc ggcacgcagc ccggggccgg cggggacgcg cagggtgtct acgacgccgg
     3181 ggctcggcgc gaagccggcc tcgggatcgc gggcggagag ggggacctgg agggcgtgcc
     3241 cccgcggctc gggaggagcg gcgccgtcga gccgcccgcc gcgggagagg tgggtgtgga
     3301 gcgcggcgag gtcgaggccg gtgagggcct cgaccgtggc gcggcagggg
//
LOCUS       DASPJI010000361        14949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_485791, whole genome shotgun sequence.
ACCESSION   DASPJI010000361 DASPJI010000000
VERSION     DASPJI010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14949)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14949
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485791"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VEL74_09625"
     CDS             <1..471
                     /locus_tag="VEL74_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62828.1"
                     /translation="EALGHFDTCSDCRSRAIAADPTLLFRRVPALELSPSQEAAEVEA
                     VRNAVAAMRTAGRVEAKARRAHRLESWPRWAAAAALAVAALSLDGDDAWRLRPVPAPA
                     DRVEVEAMAPAPAVPAGTELAPAPRFEDLSRPWVYHEEGKDFAVAMFIAEDLEI"
     gene            523..1236
                     /locus_tag="VEL74_09630"
     CDS             523..1236
                     /locus_tag="VEL74_09630"
                     /inference="COORDINATES: protein motif:HMM:NF015891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HYG62829.1"
                     /translation="MLPVRRIVVALLWALLMGPALLAQPSGIPDERVLYAHTFKHQPA
                     AEAVEIVRPLLSKRGTVELQPGDNTLVVRDTAAHIKRIAPVLQSYDHPPLPLDLEIFI
                     VKASRSSATPPVRRSGLPEPITRRLREMLPFDIYDIRARAYLASSEGQAVTYGMGKEF
                     EVSFRMGTLVDDGRVRLTDFQVARRLSAGGTRPLIHTTLNLWLDQPMSLGFARSESSR
                     EALMVVLSLKRGDARKVQR"
     gene            1245..2462
                     /locus_tag="VEL74_09635"
     CDS             1245..2462
                     /locus_tag="VEL74_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYG62830.1"
                     /translation="MQFVCRVGTPDGRVIEDVFNASDESALRADLGKRGMHIFEVRRR
                     GVPQRMSVPSLGRRRKRIKDQEFVIFNQELAALLKAGLPLLQALELMLERMHDPHFKS
                     VLTEIRDQVKSGADLSDAFASHGEMFPRLYPSSLKAGERSGELEQVIRRFIRYMRLVL
                     DARRRVVSALIYPAVLVGLSAAMIAVMAVYVVPKFMVFFENLDAELPLVTRLLLAVSG
                     FANRNWLLLLGGTVAVVMALRSWNRTDAGRLYLDGLRLKLPILGGVLHRFSLAEFCRS
                     LSTLLSGGIPLVPAFEIAVSSVGNTWVRSRIEPNIQMVREGKAFHAALEKSEVFTDMS
                     IDMVKVGEATGSLDEMLSNVADFLDEQIETRMQRILSLIEPMMLVFMGIIIAILLVAI
                     YLPLFGLLGQSKF"
     gene            2501..4171
                     /locus_tag="VEL74_09640"
     CDS             2501..4171
                     /locus_tag="VEL74_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HYG62831.1"
                     /translation="MVVLNGKAAPPARAQVKEPEVETPEQARQRLVLERREAEAFAER
                     YGLEFVDMSRFRIDNDLFRRVPFELMLRYGFIPEMQLEGRMAVVMADPSDVTKIDEME
                     MLLGQPVEVKVGVRSAIEEILQKSESAQRVLDEASEDFRIQLVQEDEDGEEILSIDRI
                     TADTSPIIKLVDSTMFNAIQRRASDIHIETRDTEVIIKYRIDGVLYQAMEPIDKRHHQ
                     TIISRIKVMSELDIAEKRIPQDGRFKLRLKGRTIDFRVSIMPSVHGEDCVIRILDKES
                     MNKEFKNLRLDILGFDEETMRKVRKFIREPYGMVLVTGPTGSGKTTTLYACLSEIQTS
                     EDKIITIEDPVEYQLRGITQIPVNEKKGLTFARGLRSILRHDPDKVMVGEIRDEETAQ
                     IAIQSALTGHLVFTTVHANNVVDVLGRFLNMNVDLYNFVSALNCVLAQRLVRKICPHC
                     RRPAKLPPQVLEESALDPAVYADFTFHEGAGCIECNGTGFLGRLAVSELLDLSDHIRE
                     LILDRRPASEIKRAAKEEGMTFLRESALEMVFRGVTTLREINKVTFVD"
     gene            4140..5129
                     /locus_tag="VEL74_09645"
     CDS             4140..5129
                     /locus_tag="VEL74_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62832.1"
                     /translation="MRSTKLPSWIERLLGFDPVPAPPHVLALDAERLRYGQFLRDRHG
                     WRFRHFREVALPRDAFHHGPLGGPLRDPAAFQELARGLLADIPGGVTEASLVIPDGWL
                     RVTFTESGELPRAGEARDEVLRWKLRRLVPFRVEELRLAAVEVTPLAVQEEPQRLLLG
                     FAVEQLLAQVEEAFTALGVRIGRVTNTSLALLSALDVARPGSLAAMVLVDEEGYTLVF
                     SRGDEPVLHRYKGLNRTLPEGARTSFVARDLKLTRNFLDEQYAGTGIAPLLLVAPPEL
                     EPLWVDRLEEGLGRDAAPLDGRHLPPLRAEEPVPAPPWRVLAPMLGAARQEVP"
     gene            5126..5962
                     /locus_tag="VEL74_09650"
     CDS             5126..5962
                     /locus_tag="VEL74_09650"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HYG62833.1"
                     /translation="MSTAEPVQKTAAPAAAGPAPAARRHEALNLARRPFVNTRPAVRV
                     ALLLWAVGLLLLFGNVTLFWSYLASSGEMRAELDRTEEQIDTERQTVSRLRQRLEAAE
                     LAELNEQVEYLNTKIDERTFSWSLLFDRIAEVLPNDVHLQRLAPSGVVQDDKRRTARR
                     TGEDDGTVALAISGAAKSDEALTRVLDNLFAHPAFPDANLSRESRSEETGEIEFEVTA
                     SYLQDLEPVQGAAAGRVEVIEETPAAGATPTPGQIFGQAPGTAPGPTPGPAQPAPQGG
                     RS"
     gene            5959..6591
                     /locus_tag="VEL74_09655"
     CDS             5959..6591
                     /locus_tag="VEL74_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62834.1"
                     /translation="MSGRESIWRLRLWVWLPALIFFLANVVVFTVYRLGYAGTVQSLE
                     RDLEDEKVELEKQASERRRLEELLAKAETNRRQIQQLYGQRFSTRQARLTLIDREVKT
                     LARQAGMAPRSVEYLQQEIEDFNLIKRSFNFSVHGTYPELRQFINLLELSDSFLILEA
                     MTLSQGTGEDPRLRIGLTLSTLFEDRSRTPAVPPASRARQAAASGVGGVP"
     gene            6588..7250
                     /locus_tag="VEL74_09660"
     CDS             6588..7250
                     /locus_tag="VEL74_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62835.1"
                     /translation="MNATPRQKVLLGILGFLLVFALWQTFGPSDDGGTAPPPAAVELE
                     DQEDGPARPQLPGSRRSRQRETVEEVTVLRVADLDIQARDYAPGRDPWRYYEPPPPGP
                     SPEELARQRAEAERLRLLEEQRRREAEQRQAEMLAQQPPPEPPVPQPPPFPYKYLGNF
                     GPPGRQIAVFSDGEGKTIINRRQGDVIDGKFVVAQIGYESVDIQFVGFPNTPAQRLPV
                     GR"
     gene            7269..9935
                     /locus_tag="VEL74_09665"
     CDS             7269..9935
                     /locus_tag="VEL74_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HYG62836.1"
                     /translation="MRDPNVRRSISILLLVALVAVSGCTSYRAAREAETAAKMGNWDD
                     AVLHYLAALEEDPNNVEYRAALLRAKIQSSREHFEKGQEFEKAGVIERALVEYQQAVQ
                     LDPTNQYAMARLEQARQAYNAQRQGRGATSLEQLKESTRASRPQPPELNPRSKDPIDV
                     NFPEPTSIFEIYRALGQAFGINVMFDPNLKDQEIAIELKQVTAQAALETLIRTAGHFY
                     KVMDDHTVIIAQDTPQNRRVYEDLVIQTFFLSNAEVKDMMTILRSLIDARKIATNEQL
                     NAIILRDTADKVKVAERIIEANDKSKAEVVVDVELLQIDSARLRNLGVSLTEYSVGQS
                     LDTGVGTGTGGTGGGTGGGSLTTPIRLSDIEFLNQSNWILTIPNFIYNFVKRSTQAQI
                     LARPQLRITDGEKANLIIGDKVPIPLTTFNTQNVGTQGGIVPITSFQYQDVGIRIDLE
                     PRVHHNQEVTLKIKVEVSNLSGTVEGSGGQEQPIIGTRTIESVIRLQDGETNFLAGLI
                     RTADVDSDSGFPGLSEIPVLGRLFSSKSTDRQRTDVILTLTPHIIRNAQITEEDLLPI
                     WVGTEANVSFRGGSPRVESDVEGPFDGNEGTPEEIQDAIRRRIQRLPRGLRPGEGGMP
                     DDIIQEEEPAPQPPPPPTGVELVPATPPTDIFRPPAPAPTEEPPVDEEPPAGADLARL
                     QPTAAEAGMALASQKSTATQAGEARARGAAVRLWLAPQRLEVAPGDRFEMRVQVAAGQ
                     PISHLPLSLDFDARMLAVEKVEAGDFLGGEGEAQVMSDFSRPGSLVVGASRMGQVPGV
                     KGDGTVARITFRALAKGSTRIGFVTGKALDASLKPVASSLRPARVEIGEIEPDAEALE
                     SEQETDQPASPAASRPRTAALLPREASPAAGR"
     gene            9958..10425
                     /locus_tag="VEL74_09670"
     CDS             9958..10425
                     /locus_tag="VEL74_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HYG62837.1"
                     /translation="MMARRRAQSGYTLAELVMVAAVMVTLASVTLPVVKFTAKRTKEM
                     ELRAALREMRSAIDEYKRYSDMGLVPVDLGTEGYPAELEVLVEGVELVGQVDKKHKFL
                     RRIPIDPMTGKDEWGLRSFQDEFDSTSWGGEDVFDVYSLSAGVGLNGVPYRKW"
     gene            10469..10888
                     /locus_tag="VEL74_09675"
     CDS             10469..10888
                     /locus_tag="VEL74_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYG62838.1"
                     /translation="MNERRESGFTLLELIIVVAIIGILATVAMPALKDVPRRSSEAVL
                     KNNLRTLRDVIDQHYGDKGKYPTSLEALVEGGYLRKIPEDPITKRSDTWQLVYEEIDP
                     DNPPAESEESEDGQPGIVDIHSGSPLTSLDGTPYSEW"
     gene            10894..11745
                     /locus_tag="VEL74_09680"
     CDS             10894..11745
                     /locus_tag="VEL74_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62839.1"
                     /translation="MRSSHPGRGESGYNLVVLIVIITVMNVMVAAALPLWSGAIQRDK
                     EEELIFRGLQYAEAIRVFQNKQSRLPVRLEELIEAEPRAIRQLWKDPMTEDGKWQLIF
                     QNMQGAPLQPQPGGIGGDPAAGEDPAARPRPRRERDDDEGESENNGRDGDGDSGFGGP
                     AKGDQVAVGPIIGVRSRSTKASFITWNGRQRYDEWQFTINLLNQPRPSPQPPPGGFDE
                     NMMGGRIDLSNRWIGRPLPPHLTQGQPQDGNLPNDPMQLGPNRRKPSGPGAPGNRRRP
                     SSGSDDE"
     gene            11742..12185
                     /gene="dut"
                     /locus_tag="VEL74_09685"
     CDS             11742..12185
                     /gene="dut"
                     /locus_tag="VEL74_09685"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HYG62840.1"
                     /translation="MKVRIQRLPAALDLPLPTPASPGSAGFDLRAAVDGELVLRPGER
                     ALVPTGLRLEIPPGWEGQVRPRSGLALRHGIGVANAPGTIDSDYRGEVGVILINLGES
                     PFSVNRGDRVAQLVIAPVQRVEWDEVDALEPSARGEGGFGSTGVG"
     gene            12182..14701
                     /locus_tag="VEL74_09690"
     CDS             12182..14701
                     /locus_tag="VEL74_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HYG62841.1"
                     /translation="MTGRPAALLDSWFEQRGWASFEFQRQVWEAYLAGESGLVHAATG
                     TGKTYAVWLGPLLEWLAENPDGSAEQAAKRPVRPARRQTTPPLRVLWITPLRALAADT
                     EGSLRAPLAGLGIPWSLERRTGDTSSALRSRQRGRLPTALVTTPESVSLFLSYPEARE
                     LFSTLHLVVVDEWHELLGGKRGVQTELALARLRRWCPGLRIWGLSATLGNVDEALRTL
                     LGEGARPGRVVRGLDAKPVRIDSLLPDAVERFPWAGHLGLALLPRAVAAAEEGQSALI
                     FTNTRSQTELWYQAILDARPDWAGEVALHHGSLDRKVRDWVEEALREGRLRCTVCTSS
                     LDLGVDFAAVDRVLQVGSPKGVARLLQRAGRSGHRPGAESRVTCVPTHAFELVEVAAA
                     RRAIGDGRIEPRTPQSKPLDVLAQHLVTAAMGGGFEAGEMLAEVRTARAYHDLTAAEW
                     EWVLDFVTRGGPALAAYPEYRRVAENDGRYGVADRGVAHRHRLSIGTITSDASVVVQY
                     LSGGRLGTVEESFIARLRPGDRFVFAGKTLELESVRDLTAYVRKARGRSSTVPRWLGG
                     RLPLSTHLAAAVREELEAARDGRFEGAEMEAVRPLLELQARWSILPAADELLIERLRS
                     REGHHLFIYPFEGRLVHEGLAALFAWRLARRRPLTFTLAVNDYGFELLAADPAPLEEA
                     VADGLLSPEGLVDDIPASLNAAEMAKRQFREIARIAGLLFEGLPGRRKPARHLQASSG
                     LFYEVFTQYDPGNLLLLQAHREVLEQQLERSRLGRTLARLSDSRLTVVELARPSPLAF
                     PLLIERLRATLSSEKLADRVRRMQAQLEKAAGPIERKGRRR"
     gene            14698..>14949
                     /locus_tag="VEL74_09695"
     CDS             14698..>14949
                     /locus_tag="VEL74_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYG62842.1"
                     /translation="MRAGDVRIDIAGEPLVLLPERALLWERRATLVVADAHLGKAAAF
                     RAAAIPLPGGTTTEALGRLAAALSRTGARRLLLLGDFFHA"
BASE COUNT         2279 a         5268 c         5279 g         2123 t
ORIGIN      
        1 gaggctctcg ggcacttcga cacctgctcc gactgccggt cccgcgccat cgcggccgac
       61 ccgaccctgc tcttccggcg ggtgccggcg ctggagctgt cgccgagcca ggaggcggcc
      121 gaggtggagg cggtgcgcaa cgccgtcgcc gccatgcgca ccgccgggcg ggtggaggcg
      181 aaggcccgcc gcgcccaccg gctcgagagc tggccgcgct gggcggccgc ggccgcgctg
      241 gcggtggcgg ccctgtccct ggatggcgat gacgcctggc gcctccggcc cgtgccggcg
      301 ccggccgaca gggtggaggt cgaggccatg gcgcccgccc ctgccgttcc tgccggcacc
      361 gagctggcgc cggccccgcg cttcgaggac ttgagccggc cctgggtcta tcacgaggaa
      421 ggcaaggact tcgccgtggc gatgttcatc gccgaggacc tcgagatctg acgccggctc
      481 tgtcgttcct acctgttttg cgctaagtta ctggattctc cgatgttgcc cgttcgccga
      541 atcgtcgtgg ccctcctttg ggccttgctc atggggccgg ccctgctggc ccagccgtcc
      601 ggcatcccgg acgagcgggt gctctatgcc catacgttca agcaccagcc ggcagcagag
      661 gccgtggaga tcgtccggcc gctcttgtcg aagcgcggca ccgtggagct gcagccgggg
      721 gacaacaccc tggtggtccg cgacaccgcg gcccacatca agcgcatcgc gccggtgctg
      781 cagagctacg accacccgcc gctgccgctg gatctggaga tcttcatcgt caaggcgagc
      841 cgctcgtcgg cgacgccgcc ggtgcggcgc tcgggcctgc ccgagcccat cacccgccgc
      901 ctgcgcgaga tgctgccgtt tgacatctat gacatccggg cgcgcgccta cctcgcctcg
      961 tcggaggggc aggcggtgac ctatggcatg ggcaaggagt tcgaggtgag cttccgcatg
     1021 ggcaccctgg tcgacgacgg ccgggtgcgg ctcacggact tccaggtggc gcgccgcctc
     1081 agcgccggcg ggacgcggcc gctcatccat accaccctga acctctggct ggaccagccc
     1141 atgagcctcg gcttcgccag gagcgagtcg agccgcgagg cgctgatggt cgtgctcagc
     1201 ctcaagcgcg gcgacgcccg caaggtccag cgctaggaga cgcgatgcag ttcgtttgcc
     1261 gagtgggaac gccggacggc cgggtgatcg aggacgtctt caacgccagc gacgagtcgg
     1321 cgctgcgcgc cgacctgggc aagcgcggca tgcacatctt cgaggtgcgg cgccgcggcg
     1381 tgccgcaacg gatgtcggtg ccgtccctcg gccgccgccg caagcgcatc aaggatcagg
     1441 agttcgtcat cttcaaccag gagctggcgg cgctgctcaa ggccggcctg ccgctgctcc
     1501 aggcgctgga gctgatgctc gagcggatgc acgacccgca cttcaagtcg gtgctgaccg
     1561 agatccgcga ccaggtcaag agcggcgccg acctgtcgga cgccttcgcg tcgcacggcg
     1621 agatgttccc gcgcctctac ccgtcgtcgc tcaaggcggg cgagcgcagc ggcgagcttg
     1681 agcaggtgat ccgccgcttc atccgctaca tgcggctggt gctcgacgcc cgccggcggg
     1741 tggtctcggc gctgatctac ccggcggtgc tggtcggcct gtccgccgcc atgatcgccg
     1801 tcatggcggt ctacgtggtg cccaagttca tggtcttctt cgagaacctg gacgccgagc
     1861 tgcccctggt cacccgcctg ctgctggcgg tgtcgggctt cgccaaccgc aactggctgc
     1921 tgctcctggg gggcacggtg gcggtggtga tggcgctgcg ctcgtggaac cgtaccgacg
     1981 cggggcggct ctacctcgac gggctgcgtc tgaagttgcc gatcctcggc ggcgtcctcc
     2041 accgcttctc gctcgccgag ttctgccggt cgctgtcgac gctgctgtcc ggcggcatcc
     2101 cgctggtgcc ggcgttcgag atcgcggtct cgtcggtggg caacacctgg gtgcgcagcc
     2161 gcatcgagcc caacatccag atggtgcgcg agggcaaggc cttccacgcc gcgctcgaga
     2221 agagcgaggt gttcacggac atgtccatcg acatggtcaa agtcggagag gcgaccggct
     2281 cgctcgacga gatgttgagc aacgtcgccg attttctgga cgagcagatc gagacccgca
     2341 tgcagcggat cctgtccctg atcgagccga tgatgctggt gttcatgggg atcatcatcg
     2401 ccatcctgct ggtggcgatc tatctcccgc tcttcggcct gctcggtcag tcgaagttct
     2461 agcactgttt gaggagtagg agactcccga ggagacgaag atggtggttc tgaacggcaa
     2521 ggcggcgcct ccggcacggg ctcaggtcaa ggagcccgag gtcgagacgc cggagcaggc
     2581 gcggcagcgc ctggtcctcg agcggcgcga ggccgaagca ttcgccgagc gctacgggct
     2641 cgagttcgtc gacatgagcc ggttccgcat cgacaacgat ctgttccggc gggtgccgtt
     2701 cgagctgatg ctgcgctacg gcttcatccc cgagatgcag ctcgaggggc ggatggcggt
     2761 ggtgatggcc gacccctccg acgtcaccaa gatcgacgag atggagatgc tcctcggcca
     2821 gccggtggag gtcaaggtcg gcgtccgctc ggcgatcgag gagatcctcc agaagtcgga
     2881 gagcgcccag cgggtgctcg acgaggcgag cgaggacttc cggatccagc tcgtccagga
     2941 ggacgaggat ggcgaggaga tcctgtccat cgaccggatc accgccgaca ccagcccgat
     3001 catcaagctg gtcgactcga cgatgttcaa cgccatccag cgccgggcga gcgacatcca
     3061 catcgagacg cgggacaccg aggtcatcat caagtaccgc atcgacggcg tcctctacca
     3121 ggcgatggag ccgatcgaca agcgccatca ccagaccatc atcagccgca tcaaggtcat
     3181 gtcggagctc gacatcgccg agaagcggat cccccaggac ggccgcttca agctgcgcct
     3241 caagggacgg actatcgact tccgtgtctc catcatgccg tcggtccacg gcgaggactg
     3301 cgtcatccgt atcctcgaca aggagtcgat gaacaaggag ttcaagaacc tccgcctcga
     3361 catcctcggc ttcgacgagg agacgatgcg caaggtccgc aagttcatcc gcgagcccta
     3421 cggcatggtg ctggtgaccg ggcccaccgg ctcgggcaag acgacgacgc tctacgcctg
     3481 cctgtcggag atccagacct ccgaggacaa gatcatcacc atcgaggatc cggtggagta
     3541 ccagctgcgc gggatcaccc agatcccggt caacgagaag aagggcctga cgttcgcccg
     3601 cggcctgcgc tccatcctgc ggcacgaccc cgacaaggtg atggtcggtg agatccgcga
     3661 cgaggagacg gcgcagatcg ccatccagtc ggcgctcacc ggccacctgg tgttcaccac
     3721 cgtccacgcc aacaacgtgg tcgacgtgct ggggcggttc ctcaacatga acgtcgacct
     3781 gtacaacttc gtctcggcgc tcaactgcgt gctggcccag cggctggtgc gcaagatctg
     3841 cccgcactgc cggcggccgg ccaagctgcc gccgcaggtg ctggaggaga gcgccctgga
     3901 cccggcggtg tacgccgact tcaccttcca cgagggggcg ggctgcatcg agtgcaacgg
     3961 cacgggcttc ctcggccgcc tggccgtctc cgagctgctg gacctgtccg accacatccg
     4021 cgagctgatc ctggaccgcc gtccggcctc cgagatcaag cgtgccgcta aagaagaggg
     4081 aatgaccttc ctgcgcgagt cggcgctgga gatggtcttc cggggagtga cgacgctgcg
     4141 tgagatcaac aaagttacct tcgtggattg agcgcctcct cggcttcgat ccggtgccgg
     4201 cgccgccgca cgtcctcgcc ctggacgcgg agcggctgcg ctacggccag ttcctgcgcg
     4261 accggcacgg ctggcgcttc cgccacttcc gggaggtggc gttgccccgt gacgccttcc
     4321 accacgggcc gctcggcggg ccgctgcgcg accccgcggc gttccaggag ctggcccgcg
     4381 gcctcctggc cgacatcccg ggcggcgtca cggaggcgtc gctggtgatc ccggacggct
     4441 ggctgcgcgt caccttcacc gagagcggcg agctgccgcg ggcgggcgag gcgcgcgacg
     4501 aggtgctgcg ctggaagctg cggcgcctgg tgccgttccg ggtggaggag ctgcgcctgg
     4561 cggcggtcga ggtcacgccg ctcgccgtgc aggaggagcc gcagcgcctg ctgctcggct
     4621 tcgccgtcga gcagctcctc gcccaggtgg aggaggcatt caccgccctg ggcgtgcgga
     4681 tcggccgcgt caccaacacc agcttggcgc tgctgtcggc cctggacgtg gcgcggccgg
     4741 ggagcctcgc cgccatggtg ctggtcgacg aggagggcta cacgctggtg ttctcccgcg
     4801 gcgatgagcc ggtgctccac cgttacaagg ggctcaaccg cacgctgccc gagggtgccc
     4861 gcaccagctt cgtcgcccgc gacctgaagc tcacccgcaa cttcctcgac gagcagtacg
     4921 ccggcaccgg catcgcgccg ctgctgttgg tggcgccgcc cgagctcgag ccgctgtggg
     4981 tcgaccgcct cgaggaaggg ctggggcgcg acgccgcgcc gctcgacggc cggcacctgc
     5041 cgccgctgcg ggccgaggag ccggtccccg cgccgccgtg gcgtgtgctg gccccgatgc
     5101 tgggcgcggc ccgccaggag gtgccatgag cacggccgaa ccggtgcaga agacggcggc
     5161 ccccgctgcc gccgggcccg cccccgccgc ccggcggcac gaggcgctca acctggcccg
     5221 ccggccgttc gtcaacaccc gcccggcggt gcgcgtagcg ctgctgctgt gggcggtggg
     5281 gctcctcctc ctcttcggca acgtgacgct gttctggagc tacctcgcca gctccggcga
     5341 gatgcgcgcc gagctcgacc ggacggagga gcagatcgac acggagcggc agacggtctc
     5401 ccggcttcgg cagcggctcg aagccgccga gctggcggag ctgaacgagc aggtggagta
     5461 cctcaacacc aagatcgacg agcgcacctt ctcgtggagc ctgctcttcg accgcatcgc
     5521 cgaggtgctg cccaacgacg tccacctcca gcggctggcg ccctccggcg tggtgcagga
     5581 cgacaagcgg cgcaccgcgc gccgcaccgg cgaggacgac ggcacggtgg cgctggcgat
     5641 ctccggcgcc gccaagagcg acgaggcgct gacccgcgtc cttgacaacc tcttcgcgca
     5701 cccggccttc cccgacgcca acctctcccg tgagtcgcgg agcgaggaga ccggtgagat
     5761 cgagttcgag gtgaccgcga gctatctcca ggacctcgag ccggtgcagg gagcggcggc
     5821 ggggagggtg gaggtcatcg aggagacgcc cgcggcggga gccaccccca ccccgggcca
     5881 gatcttcggt caggcacccg gtacggcgcc cggcccgacg ccgggtccgg cccagccggc
     5941 gccccagggg ggacggtcat gagcggccgc gagtccatct ggcggctgcg gctctgggtc
     6001 tggctgccgg ccctgatctt cttcctggcc aatgtcgtgg tgtttaccgt ctatcggctg
     6061 ggctatgccg gcaccgtgca gtcgctggag cgcgacctgg aagatgagaa ggtggagctc
     6121 gagaagcagg cgtccgagcg gcgccggctc gaggagctgc tggccaaggc cgagaccaac
     6181 cgccggcaga tccagcagct ctacgggcag cgcttctcga cacgccaggc gcgcctgacc
     6241 ttgatcgacc gcgaggtcaa gacgctggcc cggcaggccg gcatggcgcc gcggagcgtc
     6301 gaatatctgc agcaggagat cgaggacttc aacctgatca agcgctcgtt caacttctcg
     6361 gtgcacggca cctacccgga gctgcggcag ttcatcaacc tgctggagct gtcggactcg
     6421 ttcctgatcc tggaggcgat gaccctttcc caggggaccg gggaggaccc gcggctgcgc
     6481 atcggcctga ccctctcgac gctgttcgag gatcggagcc ggacccccgc cgtgccgccc
     6541 gccagccggg cgcgccaggc ggcggcctcc ggggtgggag gggtgccatg aacgcgaccc
     6601 cgcgccagaa ggtgctcctg gggatcctcg ggttcctgct cgtcttcgcg ctctggcaga
     6661 ccttcgggcc gtcggacgat ggcggcaccg cgccgccgcc ggcggccgtg gagctggagg
     6721 accaggagga tggcccggcc cggccgcagc tccccggctc ccgccggagc cgccaacggg
     6781 agacggtgga ggaggtgacg gtgctgcgtg tggccgacct cgacatccag gcccgcgact
     6841 acgcgccggg gcgcgaccct tggcgctact atgagccgcc gccgccgggc ccctccccgg
     6901 aggagctggc ccgccagcgg gcggaggccg agcggctgcg gctgctcgag gagcagcggc
     6961 gccgggaggc ggagcagcgc caggcggaga tgctggcaca gcagccgccg cccgagccgc
     7021 cggtgcccca gccgccgccg ttcccgtaca agtacctcgg caacttcggc cccccgggca
     7081 gacagatcgc cgtcttctcg gacggcgagg gcaagacgat catcaatcgc cggcaggggg
     7141 acgtgatcga cggcaagttc gtcgtggccc agatcggcta cgagtcggtg gacatccaat
     7201 tcgtcggatt tcccaatacg cccgcgcagc ggctgccagt cggccgctag aacggaggat
     7261 ttttcaagat gcgtgatccc aacgtgcgga ggtcgatctc gattctcctc ctggtcgccc
     7321 tggtggccgt cagcggctgc accagctacc gtgccgcccg cgaggcggag accgcggcca
     7381 agatggggaa ctgggacgac gccgtgcttc actacctcgc ggctctcgag gaagacccga
     7441 acaacgtcga ataccgcgcc gccctgctgc gggccaagat ccaatcctcc cgcgagcact
     7501 tcgagaaggg gcaggagttc gagaaggcgg gtgtcatcga gcgcgctctg gtcgagtacc
     7561 agcaggcggt ccagctcgac ccgaccaacc agtacgccat ggcgcggctc gagcaggcgc
     7621 gccaggccta caacgcgcag cgccagggcc ggggggccac gtccctcgag cagctcaagg
     7681 agagcacccg cgcgtcgcgg ccacagccgc cggagctcaa cccgcggtcc aaggacccca
     7741 tcgacgtcaa cttccccgag ccgacctcga tcttcgagat ctatcgcgcc ctgggccagg
     7801 cgttcggcat caacgtgatg ttcgatccga acctcaagga ccaggagatc gccatcgagc
     7861 tcaagcaggt gacggcgcag gccgcgctcg agaccctgat ccgtaccgcc ggccatttct
     7921 acaaggtgat ggacgaccac acggtcatca tcgcccagga cacgccgcag aaccggcggg
     7981 tctacgagga cctcgtcatc cagaccttct tcctctccaa cgccgaggtg aaggacatga
     8041 tgacgatcct ccgcagcctg atcgacgccc gcaagatcgc caccaacgag cagctcaacg
     8101 ccatcatcct gcgcgatacc gcggacaagg tgaaggtggc ggagcggatc atcgaggcca
     8161 acgacaagtc caaggccgag gtggtggtgg acgtcgagct gctgcagatc gactccgccc
     8221 gcctgcgcaa cctgggggtg agcctcaccg agtattcggt gggccagagc ctcgacaccg
     8281 gcgtcggcac gggcaccggc ggcaccggtg gcgggaccgg cggcggctcg ctgacgacgc
     8341 ccatccggct gtcggacatc gagttcctga accagagcaa ctggatcctg acgatcccca
     8401 acttcatcta caacttcgtc aagcggagca cccaggcgca gatcctggcg cggccgcagc
     8461 tccgcatcac tgatggcgag aaggcgaacc tgatcatcgg cgacaaggtg ccgatcccgc
     8521 tgaccacgtt caacacccag aacgtcggta ctcagggggg gatcgtcccc atcacctcct
     8581 tccagtacca ggacgtgggc atccgcatcg acctcgagcc gcgggtgcac cacaaccagg
     8641 aggtgacgct caagatcaag gtggaggtgt ccaacctgag cggcacggtg gagggctcgg
     8701 gcggccagga gcagccgatc atcggcaccc gcaccatcga gtcggtgatc cgcctgcagg
     8761 acggcgagac caacttcctc gccggcctca tccgcaccgc cgacgtcgat tcggactcgg
     8821 gcttcccggg cctgtcggag atcccggtgc tggggcgcct gttctccagc aagagcaccg
     8881 accgccagcg gacggacgtc atcctgacgc tcacgccgca catcatccgc aacgcccaga
     8941 tcaccgagga ggacctgctg ccgatctggg tgggcacgga ggccaacgtg agcttccgcg
     9001 gcggcagccc gcgcgtcgag tccgacgtcg aggggccgtt cgacggcaac gagggcacgc
     9061 cggaggagat ccaggacgcc atccgccgtc gcatccagcg cctgccgcgc ggcctgcggc
     9121 cgggtgaggg gggcatgccc gacgacatca tccaggagga ggagccggct ccgcagccgc
     9181 cgcctccgcc gaccggcgtc gagctggtgc ccgccacgcc gccgacggac atcttccggc
     9241 cgccggcccc ggcgccgacc gaggagccgc cggtggacga ggagccgccc gccggggccg
     9301 acctggcgcg gctccagccc accgctgccg aagccgggat ggcgctcgcc agccagaagt
     9361 ccacggccac gcaggccggg gaggcgcggg cgcgcggggc cgcggtgcgg ctctggctcg
     9421 ccccgcaacg cctcgaagtg gcgccgggcg accgcttcga gatgcgcgtc caggtggcgg
     9481 ccgggcagcc catctcccac ctgccgctct ctctcgactt cgacgccagg atgctggcgg
     9541 tggagaaggt ggaggccggc gacttcctgg gaggtgaggg ggaggcgcag gtgatgtccg
     9601 acttcagccg ccccggctcc ctggtggtgg gggcgagccg catgggccag gtgcccgggg
     9661 tgaagggcga cggcacggtg gcgcgcatta ccttccgtgc cctggccaag ggctccaccc
     9721 gcatcggctt cgtcaccggc aaggcgctgg acgcgtccct caagccggtg gcgtcgtcgc
     9781 tgcggccggc gcgcgtggag atcggcgaga tcgagccgga cgccgaggcc ctcgaaagcg
     9841 agcaggagac ggaccagccc gcgagcccgg cggccagccg gccgcggacc gccgccctgc
     9901 tgccgcggga agcttccccc gccgccgggc gttgaaggtt ttgaggagaa cgtagcgatg
     9961 atggctcgtc gacgcgcaca gtcgggctac accctggccg aattggtcat ggtggcggcc
    10021 gtgatggtga ccctggcctc ggtcacgctg ccggtggtga agttcaccgc caaacggacc
    10081 aaggagatgg agctgcgggc ggcgttgcgc gagatgcgct cggccatcga cgagtacaag
    10141 cgctacagcg acatgggcct ggtccccgtc gacctgggca cggaaggcta cccggccgag
    10201 ctcgaggtcc tggtcgaggg ggtcgagctc gtcgggcagg tggacaagaa acacaagttc
    10261 ctgcgccgga tccccatcga tccgatgacc ggcaaggacg aatgggggct gcgcagcttc
    10321 caggacgagt tcgattcgac ctcctggggg ggagaggacg tcttcgacgt ctactccctg
    10381 tcggccgggg tcggcctgaa cggtgtcccc taccggaagt ggtagccgag cgcggccgga
    10441 gcccggaaga gagggacgga ggtgtgtcat gaatgagcgc cgcgagagcg gattcaccct
    10501 gctggagctg atcatcgtgg tggctatcat cggcatcctc gccacggtgg ccatgccggc
    10561 tctcaaggac gtcccccggc gcagctccga ggcggtcctc aagaacaacc tgcgcaccct
    10621 ccgcgacgtc atcgaccagc actacggcga caagggcaag tacccgacca gcctcgaggc
    10681 gctggtcgag ggggggtacc tgcgcaagat cccggaggac ccgatcacca agcgcagcga
    10741 cacctggcag ctcgtctacg aggagatcga tcccgacaat ccgccggccg agagcgagga
    10801 gagcgaggac ggccagcccg gaatcgtcga catccactcg ggttcccccc tcacgtcgct
    10861 ggacgggacc ccctacagcg agtggtgacg gccatgcgct cttctcatcc aggaaggggc
    10921 gaaagcggct acaacctggt cgtcctcatc gtgatcatca cggtgatgaa cgtgatggtg
    10981 gccgcggcgc tgccgctgtg gagcggagcg atccagcgcg acaaggagga ggagctcatc
    11041 ttccgggggc tccagtacgc tgaggcgatc cgcgtcttcc agaacaagca gtcgcgcctc
    11101 ccggtgcgcc tcgaggagct gatcgaggcg gagccgcggg cgatccggca gctctggaag
    11161 gacccgatga ccgaggacgg caagtggcag ctcatcttcc agaacatgca gggagcgccg
    11221 ctgcagccgc agcccggcgg catcggcggg gatcccgcgg cgggggagga tcccgccgcc
    11281 cggcctcgtc cccgccgcga gcgggatgac gacgaagggg agagcgagaa caatggccgg
    11341 gatggagacg gcgacagcgg cttcggcggc ccggccaagg gggatcaggt ggccgtcggc
    11401 ccgatcatcg gcgtgcgcag ccgcagcacc aaggcctcgt tcatcacctg gaacggccgg
    11461 cagcgctacg acgagtggca gttcaccatc aacctgctga accagccgcg gccgtctccc
    11521 cagccacccc ccggcggctt cgacgagaac atgatggggg gccggatcga cctgagcaac
    11581 cgctggatcg gccggccgct gccccctcac ctcacccagg gccagccgca ggacggcaac
    11641 ctgcccaacg acccgatgca gctcggcccc aaccgcagga agccgtccgg tccgggcgcg
    11701 cccggcaatc gccggcgccc ctcctcggga agcgacgacg agtgaaggtc cggatccagc
    11761 ggctgcccgc cgcgctggac ctccccctgc ccacgcccgc ctcgccgggg agcgccggct
    11821 tcgacctccg cgccgccgtc gacggggagc tggtgctgcg gccgggcgag cgcgccctgg
    11881 tgcccaccgg cctcaggctg gagatcccgc ccggctggga ggggcaggtc cggccgcgca
    11941 gcgggctcgc cttgcgtcac ggtatcggcg tggccaacgc tccgggcacc atcgacagcg
    12001 actaccgggg cgaggtgggg gtgatcctga tcaacctggg agagtccccc ttcagcgtca
    12061 accgtggcga ccgcgtcgcc cagctcgtca tcgcgccggt gcagcgcgtc gagtgggacg
    12121 aggtggacgc cctcgagccg agcgcgcgtg gagagggggg attcgggtcg acgggagtgg
    12181 ggtgaccggg cggccggccg ctctcctcga ctcctggttc gagcagcggg gctgggcgtc
    12241 tttcgagttc cagcgtcagg tctgggaggc ctacctcgcc ggggagagcg ggctggtcca
    12301 tgccgccacc gggactggga agacgtacgc cgtctggctg ggcccccttc tcgagtggct
    12361 ggcggagaac ccggacgggt ccgcggagca ggccgcgaaa cgccccgtgc ggccagcgcg
    12421 ccggcagacg acaccccccc ttcgcgtcct ctggatcacg ccgctccgcg ccctggccgc
    12481 cgacaccgaa ggatcgctgc gggcgccgct cgccggcctc ggtatcccct ggtccctgga
    12541 gcgccggacg ggtgacacct cgtccgccct gcgctcgcga cagcgcgggc gtctccccac
    12601 ggccctggtc accacccccg agagcgtgtc cctgttcctc tcctatcccg aggcgcggga
    12661 gctcttttcc actctccatc tggtggtggt ggacgagtgg cacgagctcc tcggcggcaa
    12721 gcggggggtg cagaccgagc tcgcgctggc gcggctgcgc cgctggtgcc ccggcctgcg
    12781 catctggggg ctctcggcca ccctcggcaa cgtcgacgaa gcactccgga ctctcctggg
    12841 agagggcgcg cgcccgggca gggtggtccg cggcctcgac gccaagccgg tgcgcatcga
    12901 ttcgctgctg ccggacgcgg tcgaacgctt cccctgggcc gggcacctcg ggctcgcgct
    12961 cctcccccgc gccgtcgccg ccgccgagga agggcagagc gccctgatct tcaccaacac
    13021 ccgctcgcag accgagctct ggtaccaggc catcctcgac gcgcgccccg actgggcggg
    13081 cgaggtggcg ctccaccacg gctccctcga ccgcaaggtc cgcgactggg tggaggaggc
    13141 gctgcgcgag ggacggctgc gctgcaccgt ctgcacgtcc agcctcgacc tgggggtgga
    13201 cttcgcggcg gtggaccggg tgctccaggt gggaagcccc aagggggtgg cgcgcctcct
    13261 ccagcgcgcc ggccgcagcg gccaccgccc gggcgccgag agccgggtaa cctgcgtccc
    13321 gacccatgcc ttcgagctgg tggaggtggc ggcggcgcgg cgggcgatcg gcgacggccg
    13381 gatcgagccg cggacgccgc agtccaagcc gctggacgtc ctcgcccagc acctggtgac
    13441 cgcggccatg gggggcggct tcgaagccgg cgagatgctc gccgaggtgc ggaccgcccg
    13501 cgcctaccac gacctgaccg ccgccgagtg ggagtgggtg ctcgacttcg tcacccgcgg
    13561 cggccccgcg ctcgccgcct accccgagta ccggcgggtc gccgagaacg acggccgcta
    13621 tggggtggcc gaccgcggcg tggcccaccg ccatcgcctc tccatcggca ccatcaccag
    13681 cgacgcctcc gtcgtggtcc agtacctctc gggaggccgg ctggggacgg tcgaggagtc
    13741 gttcatcgcc cgcctacgcc ccggggaccg cttcgtgttc gccggcaaga cgctggagct
    13801 ggagtcggtc cgcgacctga ccgcctacgt gcgcaaggcg cgcgggcgga gcagcaccgt
    13861 gccgcgctgg ctcggcggcc ggctgccgct gtcgacccac ctggcggcgg cggtgcggga
    13921 ggagctggag gcggcgcgcg acggccgctt cgaaggggcc gagatggagg cggtgcgccc
    13981 cctcctcgag ctgcaggccc gctggtcgat cctgccggcc gccgacgagc tgctcatcga
    14041 gcgcctccgc tcccgcgagg ggcaccacct cttcatctac ccgttcgagg ggcggctggt
    14101 gcacgagggg ctggcggcgc tcttcgcctg gcggctggcg cggcggcgcc ccctcacctt
    14161 caccctggcc gtcaacgact acggcttcga gctgctggcg gcggacccgg cgccgctcga
    14221 ggaggcggtg gcggatgggc tgctgtcacc cgaggggctg gtggacgaca tcccggcgag
    14281 cctcaacgcc gcggagatgg cgaagaggca gttccgggag atcgcccgca tcgccggcct
    14341 cctgttcgag gggctgccgg ggcgccgcaa gccggcccgg cacctccagg cttccagcgg
    14401 cctcttctat gaggtgttca cccagtacga ccccggcaac ctcctgctcc tccaggcgca
    14461 tcgcgaagtg ctggagcagc agctcgagcg cagccgcctc ggtcgcaccc tggcgcgcct
    14521 gtcggactcg cggctcaccg tggtggagct ggcgcgcccc agccctctcg ccttccccct
    14581 cctcatcgag cggttgcgcg cgacgctcag ctcggagaag ctggcggatc gcgtgcggcg
    14641 gatgcaggcg cagctcgaga aggcggcggg ccctatagaa aggaagggcc ggcggcgatg
    14701 agggcggggg acgtccgcat cgacatcgcc ggggagccgc tggtgctgct gccggagcgg
    14761 gcgctgctct gggagcgccg ggcgacgttg gtggtggccg acgcccatct cggcaaggcg
    14821 gcggcgttcc gcgccgcggc catcccgctg ccgggcggca ccacgactga ggcgctgggc
    14881 cggctggcgg cggcgctcag ccgcaccggc gcgcgccggc tcctgctcct cggcgatttc
    14941 ttccacgcc
//
LOCUS       DASPJI010000362         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_708769, whole genome shotgun sequence.
ACCESSION   DASPJI010000362 DASPJI010000000
VERSION     DASPJI010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708769"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VEL74_09700"
     CDS             <1..557
                     /locus_tag="VEL74_09700"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HYG62843.1"
                     /translation="FAWIYSPTEDPNVFAWDGVVPHPGSLSVEINDGANGNNWIAVDT
                     MGTVGLTQGGKVNRDGIGAVVSVLPAGGVTAMKPVLGGSSYASQDSLELLFGLGSGQD
                     AAVEVLWPGGVRNRLYGVRASERILFPEIPCSFDAEWRNQGEYVSCLARELGRLQRAG
                     VLDHEGRMRFFRSAMEAYAKRHGS"
     gene            574..1203
                     /locus_tag="VEL74_09705"
     CDS             574..1203
                     /locus_tag="VEL74_09705"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HYG62844.1"
                     /translation="MKKTNRKPWTAAAALVTAAVLSLHPAAAQKTAKEAEQPQTSPEY
                     AKLLPGENHKPLHPLVGKWEATLRGWGHGSPPPEFTFQQTLDTRWVMRNNWLQTDFTT
                     KFPQVPEPFRGIVYRGYNAATENYVAILLSDGDVRETVSKGTWDPAKKTFVFIGPEKD
                     PVTQDTFQRKEIYTLLDADSFKYQLIYVYQDGSESRAAEGTFTRVKDKK"
     gene            complement(1501..>2482)
                     /locus_tag="VEL74_09710"
     CDS             complement(1501..>2482)
                     /locus_tag="VEL74_09710"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HYG62845.1"
                     /translation="NDEVRALVARDGRRFSFDLYLDCSGFRSLLLEKSLGSPWIGFEG
                     SLFTDRAVVGPVPHGGRVRPYTTAETMSSGWCWSTPQGDADHRGYVFASAFQTPEEAE
                     MEMRRVNPGMGAARLVYFRAGRHAHFWKGNAVALGNAYGFVEPLESTALHMLIRQIGL
                     LVRSFPLRRGERGFQALLNRKVGAWWDYLRWFLAIHYRFNRRLDSPFWRACREEVDVS
                     SHAELLDAFRERGPLSYDPAARAAFDYPDPLWGPEGIDTLLLGQGVRGRLPRPALSPD
                     AWAGRLRLARSVASRAEPHERSLEILARNPELLDRMVAELRAAGPAFPAL"
BASE COUNT          411 a          858 c          895 g          318 t
ORIGIN      
        1 tcttcgcctg gatctactcc cccaccgagg acccgaacgt cttcgcctgg gacggggtgg
       61 tgccgcatcc cggctcgctg tcggtggaga tcaacgacgg cgccaacggc aacaactgga
      121 tcgcggtgga cacgatgggt acggtgggcc tcacccaggg cggcaaggtg aaccgcgacg
      181 gcatcggcgc ggtggtgagc gtgctgccgg cgggcggggt gacggcgatg aagccggtgc
      241 tgggcggctc gagctacgcc tcgcaggaca gcctggagct gctcttcggc ctcggcagcg
      301 gccaggacgc cgccgtcgag gtgctctggc cgggcggcgt gcgcaaccgg ctgtacggag
      361 tgcgggcgtc cgagcgtatc ctcttccccg aaattccgtg cagcttcgac gcggagtggc
      421 ggaatcaggg tgagtacgtg agctgcctcg cccgcgagct gggccggctg cagcgcgccg
      481 gcgtgctcga ccacgagggc cgcatgcgct tcttccggag cgccatggag gcctacgcga
      541 agcggcacgg gtcctgaagg agacgaagag accatgaaga agacgaatcg caagccctgg
      601 accgccgccg ccgccctggt gacggcggcg gtcctgtccc tccaccccgc ggcggcgcag
      661 aagaccgcca aggaggcgga gcagccgcag acctccccgg agtacgcgaa gctgttgccg
      721 ggtgagaacc acaagccgct ccacccgttg gtgggcaagt gggaggcgac gttgcggggc
      781 tggggtcacg gctcgccgcc gccggagttc acgttccagc agaccctgga cacccgctgg
      841 gtgatgcgca acaactggct gcagaccgac ttcaccacca agttccccca ggtgccggag
      901 cccttccgcg gcatcgtcta ccggggctac aacgcggcga cggagaacta cgtggccatc
      961 ctgctgtcgg acggcgacgt ccgggagacc gtctccaagg gcacctggga cccggcgaag
     1021 aagaccttcg tcttcatcgg ccccgagaag gacccggtga cgcaggacac cttccagcgc
     1081 aaggagatct acaccctcct ggacgccgac agcttcaagt accagctgat ctacgtctac
     1141 caggacggct ccgagagcag ggccgccgaa ggcaccttca cccgcgtgaa ggacaagaag
     1201 taacccgcgg tccgggtgag gggcgcgagc ccctcacccc ggacgctgcg attccatccc
     1261 tttgaacagg acgccgtccg gaacgctcgg gaagcttccc gggccgtccg gattccagtc
     1321 gcttgaacgg gacgaggtcc ggagcgcccg gactccatcc ggatgaagcg gacgcgatcc
     1381 ggaacgcccg ggacgcttcc cgggccgtcc ggatgccatc cgcggcgtcc ggacgggatc
     1441 cccggcgtcc gggagagatc cgcggcaccc ggacggaatc cgaagggtga agatcgaatc
     1501 tcagagcgcc gggaacgccg gccccgccgc gcggagctcg gcgaccatcc ggtccagcag
     1561 ctcgggattc cgggcgagga tctccaggct ccgctcgtgc ggctcggcgc gggacgccac
     1621 cgagcgggcc aggcgcagcc ggccggccca ggcgtccgga gagagcgccg gacgcggcag
     1681 cctccccctc accccctggc ccaggaggag ggtgtcgatc ccctcgggcc cccagagcgg
     1741 gtcggggtag tcgaaggcgg cgcgggcggc agggtcgtag gagagcgggc cgcgctcgcg
     1801 gaaggcgtcc agcagctcgg cgtgggagga gacgtccacc tcctcgcggc aggcgcgcca
     1861 gaacggggag tcgagccgcc ggttgaagcg gtagtggatg gccaggaacc agcgcaggta
     1921 gtcccaccag gcccccacct tgcggttgag gagcgcctgg aagccccgct ccccgcgccg
     1981 tagcgggaag ctccgcacca gcagcccgat ctggcggatc agcatgtgga gcgccgtgga
     2041 ctccagcggc tcgacgaagc cgtaggcgtt gcccagcgcc acggcgttgc ctttccagaa
     2101 gtgggcgtgc cgccccgccc ggaagtagac gagccgcgcc gcccccatgc ccgggttgac
     2161 gcgccgcatc tccatctcgg cctcctcggg ggtctggaac gccgaggcga agacgtagcc
     2221 gcggtggtcg gcgtcgcctt gcggcgtgct ccagcaccag ccgctgctca tcgtctccgc
     2281 cgtggtgtac gggcgcaccc ggccgccgtg cggcaccggc ccgacgacgg cgcggtcggt
     2341 gaacaggctc ccctcgaatc cgatccaggg ggagccgagg ctcttctcca ggaggagcga
     2401 gcggaagccc gagcaatcca gatagaggtc gaaggagaag cggcggccgt cgcgggccac
     2461 cagggcgcgc acctcgtcgt tg
//
LOCUS       DASPJI010000363        10357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_367245, whole genome shotgun sequence.
ACCESSION   DASPJI010000363 DASPJI010000000
VERSION     DASPJI010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10357)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10357
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367245"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /locus_tag="VEL74_09715"
     CDS             complement(<1..1511)
                     /locus_tag="VEL74_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYG62846.1"
                     /translation="MASKDLQNISELESRKVAEASREQEWSQPSFLRELFLGNFRLDL
                     IHPYPLPEQERPEFTAFYNAMREFLRDHVDPVEIDATGEYPESVVDGLRKLGAFGMKI
                     PKKYGGLGFTNVEYQKIMLLLGSIDGNLSALLSAHQSIGVPQPLKLFGSDQLKQKYLP
                     RCAKGEISAFALTEAQVGSDPARLETTAEKTADGSAYILNGEKLWCTNGTIAKLLVVM
                     ARDPKTKKISAFVVEADAPGVKVEHRCHFMGLRALANAVISFKSVRVPAENLIGAEGK
                     GLKIALTTLNDGRLSIPNGSVGTSKLCIEICRKWASERVQWGKAVGKHEAIAHKIADM
                     AATTFAMESIAQLATEMSDRGGYDIRLEAAACKEWNSERTWEIVDDTMQIRGGRGYET
                     EKSLAARGEEPIGVERIMRDYRINRIFEGSSEIMHLFMAREAVDKHLEVAGALIDPEK
                     SMNEKLSALPGMAAFYASWYPTRWLGWGQWPRYAEFGDLAGHLRFVERSSRKLS"
     gene            complement(1730..2632)
                     /locus_tag="VEL74_09720"
     CDS             complement(1730..2632)
                     /locus_tag="VEL74_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYG62847.1"
                     /translation="MSSTSPSRPVVYASVGVLILIWGTTWAAIRIGLEGIPPLTGVTL
                     RFAIAAAVLLPLAPALGVRLGRSQVERRLWLASTVLNFCASYGILYWAEQWVPSGLTA
                     VLFATFPLWMGLLAHLLLPGERLTPRAFAGALAGFAGVAVIFSEDFRSLGGPQVAFAA
                     AVVLLSPVSSALGSVAIKRWGQGIHPLSLSAVPMGLTALVMAPAAWLLERGREVTFDT
                     RSVAALVYLAVFGSAITFTLYYWLLKRLPATSLALITYATPVVAVVVGSLFLDEPFTL
                     RILLGALLVIAGVAVAMRPRPKAA"
     gene            complement(2646..4007)
                     /gene="asnS"
                     /locus_tag="VEL74_09725"
     CDS             complement(2646..4007)
                     /gene="asnS"
                     /locus_tag="VEL74_09725"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HYG62848.1"
                     /translation="MSETTPVTPAPAEAPATPEASAPVPVTSVRELRQNVGRRVELSG
                     WLDNKRSSGKIAFLQVRAGGSTVQAVAGRNDLPEQEWAEVERVTQESTIRVVGTVKED
                     KRSPIGVEIQMSGFSILQLTEDYPITPKEHGTAFLMEHRHLWLRSSRQRAALRVRSEV
                     EQAIRDFFYERDFTLIDSPILTPAACEGTSTLFETDYFGDKAYLSQSGQLYLEPAAAA
                     LGKVYCFGPTFRAEKSKTRRHLMEFWMVEPEVAFLEFEGLCQLAEDFVVYLAGRVLER
                     CQEQLKVLERDTAKLQQILPPFPRITYTQAVEILNRAGNPIQWGDDFGADEETLVASQ
                     YDRPVMVTHFPAAFKAFYMQPAPDNPEVVLGMDVIAPEGYGEIIGGSQRIHDHDLLLQ
                     RIQQHGLPIEAFQWYLDVRKYGGYPHSGFGMGIERFVAWMTGLTHLRETIPYPRMLYK
                     IYP"
     gene            complement(4026..4721)
                     /gene="rnc"
                     /locus_tag="VEL74_09730"
     CDS             complement(4026..4721)
                     /gene="rnc"
                     /locus_tag="VEL74_09730"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HYG62849.1"
                     /translation="MAFGRRPTSPFERRLGYRFKRLDLLELALTHRSWANEKSLPEHY
                     ERLEFLGDAVLGMITAEWLFQNHPELPEGELSKLKAQLVSGTSLARHAEKLKLGEELR
                     LGVGEERSGGRTKSSLLADSLEAVFGAIFLDNGLDAARAVVLPMIEEASGEKAILQTR
                     DAKTQLQEIAQALGWDLPEYRLVDSAGPDHSKVFTVECWLNGECSGRGDGPSKKLAEQ
                     RSAAEALARMPES"
     gene            complement(5171..7006)
                     /gene="mutL"
                     /locus_tag="VEL74_09735"
     CDS             complement(5171..7006)
                     /gene="mutL"
                     /locus_tag="VEL74_09735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HYG62850.1"
                     /translation="MARIQLLPDPLVSQIAAGEVVERPASVVKELLENALDAGARTVE
                     VQLEGGGKSRIAIADDGCGMGRDDALLAFDRHATSKIGSFEDLERVATLGFRGEALAT
                     IAAVSRVELTTADQPGDGHRVVIEGGRVRVAEPFSRPRGTTLDVRSLFFNVPARRKFL
                     KSPQTELRRAVEVVQGYALAREDVRFCLVHEGRDLLDVLPAPPSTGGARERIGQIFGT
                     DFAAQLVDIPREEGRHGEVIGGFVGMPATARGRRQFVYVNRRLIRDRAVLAAFYRAVR
                     EEWRSEDFPALFLFLDVPPEEVDVNVHPQKAEVRFRDPGLVDRVHEAFRRALEKARGE
                     EPAPLRPPSSEPVVPFAWQGLGERRRPETPWGSREWAAPVAETAQAAPLSAAAAPVPE
                     PQTRLATPSYTPVERPMVPLSGRRGEARPFRLLGQYKGTLILLEGPDGLYLIDQHVAH
                     ERILYERLRRALLAEATPVQHLLTPVLLELGPAERLRLLELAPSLDDCGFGVASLSGN
                     TLALTSVPAVLSADEAESLVRALAGEEGANQAAHDLRRQILEALAASLACKGAVKMHH
                     PLAPLEMEALVAELFVAEQPYACPHGRPIVLQMTDSDLERRFGRR"
     gene            complement(7006..7353)
                     /locus_tag="VEL74_09740"
     CDS             complement(7006..7353)
                     /locus_tag="VEL74_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62851.1"
                     /translation="MDDSERSYGYSEDIYHGKKPGEESYEYGPEPEGLDGNVNEEVSP
                     EEAAMHIEDRGLATGRGEESPLDAGDTDGTGGTYDYDRSGASGNVESFTWSQPDSEAK
                     EEELAETTVQSRP"
     gene            complement(7449..7721)
                     /locus_tag="VEL74_09745"
     CDS             complement(7449..7721)
                     /locus_tag="VEL74_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62852.1"
                     /translation="MKQGVAMAELIHTHTAVFRDEAGVVYSIQTRGEERPDGTWSGWL
                     EFHPVDPGLPVRTTGQETTQPNRDALVYWATGLEPLYFDGAFDRAG"
     gene            complement(7737..>10357)
                     /gene="ppdK"
                     /locus_tag="VEL74_09750"
     CDS             complement(7737..>10357)
                     /gene="ppdK"
                     /locus_tag="VEL74_09750"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HYG62853.1"
                     /translation="AHLLRLERQSGRRFGDPAKPLLVSVRSGAPRSMPGMMDTILNLG
                     LSEEIVEASIARGTDARFVLDAYRRLISMYGDVVLRVPHGGFEKILAAARQEHGAASD
                     ADLPAEALREVSRRYLDLVAAHGTPFPQQPRQQLWGAIGAVFQSWNNQRAREYRRIEG
                     IPETFGTAVNVQTMVFGNRGADCATGVAFTRNPATGERRVYGEYLVNAQGEDVVAGIR
                     TPRPILADDGKGGLGADFPEAHRDLEKVCEVLERHFRDMQDVEFTIEHGKLFMLQTRS
                     GKRTGPAAVRIAVEMVEEGLIDRREALSRVEPQQLGQMLAPEFDAEEKKRAVAGRDLL
                     AKGLPAGPGAASGRIALTAEDAAAMAASGPVLLVRAETSPEDIVGMHASAGILTSRGG
                     MTSHAAVVARGLGKPCVVGAGDLEIDEETGDVRLGDRVFRPGDQLSIDGTTGEVIAGA
                     LSTRPSEVLRSLLDGGEITSPAAHAFFEILDWADAERRLEVRANADTPHDARVARALG
                     AQGIGLCRTEHMFFADDRIPWVRQMILAETAELRDKALAALLPMQQKDFEGIFAALSG
                     LPITVRLLDPPLHEFLPHGQKALRQLAGQMGVDPEEVEARAHALEEANPMLGHRGCRL
                     GLTTPEIYDMQVEAIVRAACVRRRAGDDVRPEIMVPLVGTETEMVQLRERTAAVVRRV
                     LAEEGTELPVLIGTMIEVPRAALVADKIARHADFFSFGTNDLTQMTYGYSRDDSGRFL
                     PKYLEAKILECDPFQRIDEEGVGQLVRMASELGRGTNPKLHLGICGEHGGDPQSVDFF
                     DRVGLEYVSCSPYRVPIARLAAARATLNRAAEKAALEKQAAEKPARKPEPAKGGRRPA
                     ASKVEAPVPVMAAPRS"
BASE COUNT         1456 a         3581 c         3770 g         1550 t
ORIGIN      
        1 gacagcttgc ggctgctgcg ctcgacgaac cgcaggtgcc cggcgaggtc gccgaactcc
       61 gcgtagcgcg gccactgccc ccagcccagc cagcgggtcg ggtaccagga ggcgtagaag
      121 gccgccatgc cgggcagcgc cgagagcttc tcgttcatcg acttctcggg gtcgatcagt
      181 gcgcccgcca cctcgaggtg cttgtccacg gcctcgcggg ccatgaacag gtgcatgatc
      241 tcgctcgatc cctcgaagat ccggttgatc cggtagtcgc gcatgatgcg ctcgacgccg
      301 atcggctcct cgccgcgggc cgccagcgac ttctcggtct cgtagccgcg gccgccgcgg
      361 atctgcatgg tgtcgtcgac gatctcccag gtgcgctcgc tgttccactc cttgcaggcc
      421 gcggcctcga ggcggatgtc gtagccgccg cggtcggaca tctcggtggc gagctgggcg
      481 atggactcca tggcgaacgt ggtggccgcc atgtcggcga tcttgtgggc gatcgcctcg
      541 tgcttgccga ccgccttgcc ccactgcacc cgctcgctgg cccacttgcg gcagatctcg
      601 atgcagagct tcgaggtgcc caccgagccg ttggggatgg agagccggcc gtcgttgagg
      661 gtggtgaggg cgatcttgag ccccttgccc tcggcgccga tcaggttctc cgccggcacg
      721 cgcacgctct tgaagctgat gacggcgttg gccagggcgc gcaggcccat gaagtggcag
      781 cggtgctcga ccttgacgcc gggcgcgtcg gcctcgacca cgaaggcgct gatcttcttg
      841 gtcttgggat cgcgcgccat caccaccagg agcttggcga tggtgccgtt ggtgcaccag
      901 agcttctcgc cgttcaggat gtaggcgctg ccgtccgccg tcttctcggc ggtggtctcc
      961 aggcgcgccg ggtcggagcc cacctgcgcc tcggtgaggg cgaaggccga gatctcaccc
     1021 ttggcgcagc gcggcaggta cttctgcttg agctggtccg agccgaagag cttcaacggc
     1081 tggggcacgc cgatcgactg gtgggcggac agcaacgccg agaggttgcc gtcgatgctc
     1141 cccaggagca gcatgatctt ctggtactcc acgttggtga agcccaggcc gccgtacttc
     1201 ttggggatct tcatgccgaa ggcgccgagc ttgcgcaggc cgtcgaccac gctctcgggg
     1261 tactcgccgg tggcgtcgat ctcgaccgga tcgacgtggt cgcgcaggaa ctcccgcatc
     1321 gcgttgtaga acgcggtgaa ctcggggcgc tcctgctcgg gcagcggata cggatggatc
     1381 aggtccagcc ggaagttgcc caggaacagc tcgcgcagga agctgggctg actccactcc
     1441 tgctcgcgag acgcctccgc aaccttccgc gactccagtt cgctgatgtt ctgcaggtcc
     1501 ttgctcgcca ttttggtctg tctccccttt ttccctggct ggggttgctt gcaatgggcg
     1561 ttccggagtc tctctccccg ggagccgccg gctccgcttg ccgcactgcg acgtcagtgt
     1621 aacacagccg ccgcggcaag gggacacaaa tctcctggaa gtagtgccac ttgcagaggg
     1681 agagatcgcg aaagagatcg gcaagccgca gtgcggcgaa ggcgtggcct caggccgcct
     1741 tgggccgcgg ccgcatggcc acggcgacgc cggcgatcac caggagggcg ccgaggagga
     1801 tgcgcagggt gaagggctcg tcgaggaaga ggctgccgac gaccaccgcc accaccggcg
     1861 tggcgtaggt gatcagggcg agcgacgtgg ccgggaggcg cttgagcagc cagtagtaga
     1921 gggtgaaggt gatggccgag ccgaagaccg ccaggtagac gagggcggcg accgagcggg
     1981 tgtcgaaggt gacctccctc ccccgctcca gcagccaggc cgccggcgcc atgaccagcg
     2041 ccgtcagccc catgggcacc gccgacagcg acagggggtg gatcccctgg ccccagcgct
     2101 tgatggccac gctccccagg gccgagctga ccggcgacag cagcaccacg gcggcggcga
     2161 aggcgacctg cgggccgccc agcgaccgga aatcctcgga gaagatcacc gccaccccgg
     2221 cgaagccggc gagcgcgccg gcgaaggcgc ggggcgtcag gcgctccccc ggcaggagga
     2281 ggtgggccag caggcccatc cacagtggga aggtggcgaa gagcacggcg gtgaggcccg
     2341 agggcaccca ctgctcggcc cagtagagga tgccgtagga ggcacagaag ttgagcaccg
     2401 tgctcgccag ccagagccgc cgctcgacct ggctccgccc caggcgcacg ccgagcgccg
     2461 gcgccagcgg cagcaggacc gccgcggcga tggcgaagcg cagggtgacg ccggtgagcg
     2521 gcgggatccc ctccaggccg atgcggatgg cggcccaggt ggtaccccag atcaggatca
     2581 ggaccccgac cgaagcgtag acgacggggc ggctcgggct cgtcgagctc aggggtggag
     2641 gtcccctacg ggtagatctt gtacagcatc cgcgggtacg ggatggtctc ccgcaggtgg
     2701 gtgaggccgg tcatccaggc gacgaaccgc tcgatgccca tgccgaagcc gctgtgcggg
     2761 tagccgccgt acttgcggac gtcgaggtac cactggaagg cctcgatcgg caggccgtgc
     2821 tgctggatac gctgcagcag caggtcgtgg tcgtggatcc tctggctgcc gccgatgatc
     2881 tcgccgtacc cctcgggcgc gatgacgtcc atccccagca ccacctccgg gttgtccggc
     2941 gcgggctgca tgtagaaggc cttgaacgcc gccgggaagt gggtgaccat caccggccgg
     3001 tcgtactggc tggcgaccag ggtctcctcg tcggcgccga agtcgtcccc ccactggatg
     3061 gggttcccgg cgcggttgag gatctcgacc gcctgcgtgt aggtgatgcg cgggaacggc
     3121 ggcaggatct gctggagctt ggcggtgtcg cgctccagca ccttgagctg ctcctggcag
     3181 cgctccagca cccggcccgc caggtagacg acgaagtcct cggcgagctg gcagagcccc
     3241 tcgaactcca ggaaggcgac ctcgggctcc accatccaga actccatgag gtggcggcgg
     3301 gtcttggact tctcggcccg gaaggtgggg ccgaagcagt agaccttgcc gagggcggcg
     3361 gcggccggct cgaggtagag ctggccggac tgggagaggt aggccttgtc gccgaagtag
     3421 tcggtctcga agagcgtcga cgtcccctcg caggcggcgg gggtgaggat cggcgagtcg
     3481 atgagggtga agtcgcgctc gtagaagaag tcgcggatcg cctgctcgac ctcgctgcgc
     3541 acccgcaaag cggcgcgctg gcggctggag cgcagccaga ggtggcggtg ctccatgagg
     3601 aacgccgtgc catgctcctt cggggtgatc gggtagtcct cggtgagctg gaggatcgag
     3661 aagccgctca tctggatctc gacgccgatc ggcgagcgct tgtcctcctt gaccgtgccc
     3721 accacccgga tcgtcgactc ctgggtgacc cgctccacct ccgcccactc ctgctcgggg
     3781 aggtcgttgc ggcccgccac ggcctggacg gtgctgccgc cggcgcggac ctggaggaac
     3841 gcgatcttgc cgctcgagcg cttgttgtcc agccagccgc tcagctcgac gcggcggccg
     3901 acgttctggc gcagctcgcg gacggaggtg acggggacgg gcgcgctggc ctcaggggtc
     3961 gcgggggcct cggcgggcgc aggagtgacg ggggtggtct cgctcatagg aggcgggatt
     4021 gtagctcaac tctccggcat gcgcgcgagc gcttccgcgg cggagcgttg ctccgccagc
     4081 ttcttgctcg gaccgtcgcc gcggcccgag cactcgccgt tgagccagca ctcgaccgtg
     4141 aagactttgc tgtggtccgg cccggcggag tcgacgagcc ggtactcggg gaggtcccag
     4201 ccgagcgcct gggcgatctc ctggagctgc gtcttggcgt cgcgggtctg gaggatcgcc
     4261 ttctcccccg acgcctcctc gatcatcggc agcaccacgg cgcgggcggc gtcgaggccg
     4321 ttgtccagga agatggcgcc gaagaccgcc tccagggagt cggcgaggag cgacgacttg
     4381 gtccggccgc cggagcgctc ctcccccacc cccagccgca gctcctcgcc gagcttgagc
     4441 ttctcggcgt gccgcgccag cgaggtgccg ctcacgagct gcgccttgag cttcgacagc
     4501 tccccctccg gcagctccgg gtggttctgg aacagccact cggcggtgat catgcccagc
     4561 accgcgtcac ccaggaattc gagccgctcg tagtgctcgg gcaggctctt ctcgttcgcc
     4621 caggagcggt gggtgagcgc cagctcgagc aggtcgagcc gcttgaagcg gtatccgagc
     4681 cgacgctcga acgggctggt gggacggcgg ccgaacgcca tggccgggga atggtagcag
     4741 gcggccgggg tcctggtagc accggccgga cgagaggtga gggggttgcc ccgctacgcg
     4801 ttaccttgtg accacaagtc gacgcagacc gatcaccatc cccaccgaaa cggaggccgt
     4861 acccgcgggg aggacgatcc tacccagggt agagccttca tccccgggga gatggcgtcc
     4921 ctaccccggg tacggtcttc atccccacgg gtacggcgat gctaccccgg gtatggcggc
     4981 cataccctgg gagatgacgg ccctacctcg ggtaggacga tcgtttggcg agggatgacg
     5041 gctctctcca cgggtatggc gatcgtctgg cgtgaaatcg cggctctcta ctcggggatg
     5101 agcttcatcc gcaggcgcgc gacgggccgg gcgaccgtcc ggatcggcgc ggaccggggg
     5161 acggccggct ttagcgtctc ccgaaccgcc gttccaggtc ggaatccgtc atttgaagga
     5221 cgatgggccg tccgtggggg caggcgtagg gctgctcggc gacgaagagc tcggcgacca
     5281 gggcctccat ctccagcggc gccagggggt ggtgcatctt gaccgcgccc ttgcaggcga
     5341 ggctcgcggc cagcgcctcc aggatctggc gccgcaggtc gtgggccgcc tggttggccc
     5401 cctcctctcc ggcgagcgcg cgcaccaggc tctccgcctc gtccgcggag agcaccgccg
     5461 gcaccgaggt cagcgccagg gtgttgcccg acagggaggc gacgccgaag ccgcagtcgt
     5521 ccagggaagg cgccagctcc agcaggcgca gccgctcggc ggggcccagc tcgaggagga
     5581 ccggcgtcag caggtgctgg accggcgtcg cttccgcgag gagcgcccgg cgcaggcgct
     5641 cgtagaggat gcgctcgtgc gccacgtgct ggtcgatcag gtagaggccg tccggcccct
     5701 ccagcaggat cagcgtcccc ttgtactggc cgagcagccg gaaagggcgc gcctcccccc
     5761 gcctccctga cagcggcacc atggggcgct ccaccggcgt gtagctggga gtcgccaggc
     5821 gggtctgggg ctcggggacc ggcgccgccg ctgcggagag cggcgcggcc tgcgccgtct
     5881 cggcgacagg agccgcccac tcccggctcc cccagggggt ctcggggcgg cgccgctccc
     5941 ccagcccctg ccaggcgaag ggcacgacgg gctccgacga cgggggccgc agcggcgccg
     6001 gctcctctcc gcgggccttc tccagcgccc gccggaacgc ctcgtggacc cggtcgacca
     6061 gcccgggatc gcggaagcgc acctcggcct tctgggggtg gacgttgacg tcgacctcct
     6121 ccggcggcac gtcgaggaag aggaagaggg ccgggaagtc ctcgctccgc cactcctccc
     6181 gcacggcgcg atagaaggcg gcgagcaccg cccggtcgcg gatcagccgg cggttgacgt
     6241 agacgaactg gcggcgcccc cgggcagtcg cgggcatgcc gacgaagccg ccgatgacct
     6301 caccgtgcct cccctcctcc cgagggatgt cgacgagctg ggccgcgaaa tcggtgccga
     6361 agatctggcc gatgcgctcc cgcgctccgc cagtggaggg gggcgccggc agcacgtcca
     6421 gcaggtccct cccctcgtgc accaggcaga accgcacgtc ctcgcgggcc agggcgtatc
     6481 cctgcaccac ctcgacggcg cggcgcagct cggtctgcgg gctcttgagg aacttgcggc
     6541 gcgccggcac gttgaagaac agcgaccgca cgtcgagggt ggtgccgcgc ggccgcgaga
     6601 acggctccgc gacccgcacg cgccccccct cgatgaccac gcggtggccg tcccccggct
     6661 ggtcggcggt ggtcagctcg acccgggaga cggcggcgat ggtggccagg gcctcgccgc
     6721 ggaagcccag cgtcgccacc cgctccaggt cctcgaagct cccgatcttg ctggtggcgt
     6781 ggcggtcgaa ggcgaggagg gcgtcgtccc ggcccatgcc gcagccgtcg tcggcgatgg
     6841 cgatgcggct cttgccgccc ccttcgagct gcacctccac cgtccgcgcc ccggcgtcga
     6901 gggcgttctc cagcagctcc ttgacgaccg aggcagggcg ctccaccacc tcgccggcgg
     6961 cgatctggct gacgagcggg tcggggagga gctggatcct ggccatcagg gccgggattg
     7021 taccgtcgtc tccgccagct cttcctcctt cgcctcggaa tcgggttgac tccaggtgaa
     7081 cgactcgaca ttgccggacg cgccgctgcg gtcgtagtca taagtgccgc ccgtgccgtc
     7141 ggtatcgccg gcatcgagcg ggctctcctc gccgcgcccc gtggcgagcc cccggtcctc
     7201 gatgtgcatg gcggcctcct ccgggctcac ctcttcattg acgttgccat ccagcccctc
     7261 gggctccggc ccgtactcgt agctctcctc gccgggcttc ttcccgtggt agatgtcctc
     7321 gctgtatccg taactccgtt ccgaatcgtc catgggccgg acctcctgct cgtaaaaggt
     7381 tctcccccct ccgcgagggg ccgcgaaggg gggagtgcaa agagcaggcc gcccggctcc
     7441 ggctggacct aaccggcgcg gtcgaacgcc ccgtcgaagt agaggggctc caggccggtg
     7501 gcccaataga cgagggcgtc gcggttgggc tgggtggtct cctgcccggt ggtgcggacg
     7561 ggcaggccgg ggtccaccgg gtggaactcg agccaccctg accaggtgcc gtccggccgc
     7621 tcctcccccc gggtctggat gctgtagacc acgcccgcct cgtcgcggaa caccgccgtg
     7681 tgggtgtgga tcagctcggc catggcaacc ccctgcttca tggtgtacct ccgtcctcag
     7741 ctccggggag cggccatcac cgggaccggc gcctcgacct tggaagcggc cggccgcctc
     7801 ccccccttcg ccggctccgg cttgcgggcg ggcttctccg cggcctgctt ctccagggcg
     7861 gccttctcgg cggcgcggtt gagggtcgcc cgcgcggcgg cgaggcgggc gatgggcacc
     7921 cggtacggcg agcaggagac gtactccagg cccacgcggt cgaagaagtc caccgactgc
     7981 ggatcgccgc cgtgctcgcc gcagatgccc aggtgcagct tcgggttggt gccgcggccc
     8041 agctcggacg ccatgcggac gagctgcccc accccctcct cgtcgatgcg ctggaagggg
     8101 tcgcactcca ggatcttcgc ctccaggtac ttcggcagga agcggccaga gtcgtcccgg
     8161 gagtagccgt aggtcatctg ggtgaggtcg ttggtgccga aggagaagaa atcggcgtgg
     8221 cgggcgatct tgtcggcgac cagggcggcg cgggggacct cgatcatggt gccgatgagc
     8281 accggcagct cggtcccctc ctcggcgagc acgcgccgga ccacggcggc ggtgcgctca
     8341 cggagctgga ccatctccgt ctcggtcccc accaggggga ccatgatctc gggccgcacg
     8401 tcgtcgccgg cgcgccggcg gacgcaggcg gcgcggacga tggcctccac ctgcatgtcg
     8461 tagatctccg gcgtggtgag gcccaggcgg cagccgcggt ggccgagcat ggggttggcc
     8521 tcctccaggg cgtgggcgcg ggcttcgacc tcctcggggt cgacgcccat ctggccggcg
     8581 agctggcgca gggccttctg gccgtggggg aggaactcgt ggagcggcgg atcgaggagc
     8641 cgcacggtga tcgggaggcc cgagagggcg gcgaagatcc cctcgaagtc cttctgctgc
     8701 atgggcagga gggccgccag cgccttgtcg cgcagctcgg cggtctcggc caggatcatc
     8761 tgccgcaccc aggggatgcg gtcgtcggcg aagaacatgt gctcggtgcg gcagaggccg
     8821 atgccctggg cccccagggc gcgggcgacg cgcgcgtcgt gcggcgtgtc ggcgttggcc
     8881 cgcacctcga gccggcgctc ggcgtcggcc caatccagga tctcgaagaa ggcgtgcgcc
     8941 gcgggggagg tgatctcgcc gccgtccagc aggctgcgca gcacctcgga cgggcgggtg
     9001 gagagcgcgc cggcaatcac ctcaccggtg gtgccgtcga tggagagctg gtcccccgga
     9061 cggaagacac ggtctcccag ccgcacgtcg ccggtctcct cgtcgatttc caggtccccg
     9121 gcgccgacga cgcagggctt gcccaggccg cgggccacca ccgccgcgtg ggaggtcatg
     9181 ccgccgcgcg aggtgaggat gccggccgag gcgtgcatgc cgacgatgtc ctcgggcgag
     9241 gtctcggcgc gcaccagaag gaccgggccg gaggcggcca tcgcggccgc gtcctcggcg
     9301 gtcagggcga tgcggcccga cgccgcgccg ggacccgcgg ggagcccctt ggccaggagg
     9361 tccctgccgg cgaccgcccg cttcttctcc tcggcgtcga actccggggc cagcatctgg
     9421 ccgagctgct ggggctcgac gcggctcagc gcctcgcggc ggtcgatcag cccctcctcc
     9481 accatctcga cggcgatgcg cacggcggcg gggccggtgc gcttgccgct gcgggtctgg
     9541 agcatgaaga gcttgccgtg ctcgatggtg aactccacgt cctgcatgtc ccggaagtgg
     9601 cgctcgagga cctcgcagac cttctccagg tcgcggtggg cctccgggaa gtcggcgccc
     9661 aggccgccct taccatcgtc ggccaggatc ggccggggcg tgcggatgcc ggccaccacg
     9721 tcctcgccct gcgcgttgac caggtactcg ccgtagaccc ggcgctcgcc ggtggccggg
     9781 ttacgggtga aggcgactcc cgtggcgcag tcggcgcccc ggttgccgaa gaccatcgtc
     9841 tgcacgttga ccgcggtgcc gaaggtctcg gggatcccct cgatgcgccg gtactcgcgg
     9901 gcgcgctggt tgttccagct ctggaacacg gcgccgatag cgccccagag ctgctgccgg
     9961 ggctgctgcg ggaacggggt gccgtgcgcg gccaccagat cgaggtaccg ccgcgacacc
    10021 tcgcgtagcg cctccgcggg caggtcggcg tcggaggcgg cgccgtgctc ctggcgggcg
    10081 gcggcgagga tcttctcgaa gccgccatgg ggaaccctca ggacgacatc gccgtacatg
    10141 ctgatcaggc ggcgataggc gtcgagcacg aagcgcgcgt cggtgccgcg ggcgatggag
    10201 gcctccacga tctcctccga cagcccgagg ttcaggatgg tgtccatcat gcccggcatc
    10261 gagcgcgggg cgccggagcg caccgagacc agcagcggct tggccgggtc accgaagcgc
    10321 cggccggact gccgctccag ccgcaggagg tgggcct
//
LOCUS       DASPJI010000364         3784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_655433, whole genome shotgun sequence.
ACCESSION   DASPJI010000364 DASPJI010000000
VERSION     DASPJI010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655433"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VEL74_09755"
     CDS             <1..641
                     /locus_tag="VEL74_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62854.1"
                     /translation="ARHLDAVRVESSTADGVDALVWTRQFREDLQVMADEMGNDLEAQ
                     EMPLDFLPMTRIVRRLMRSEPMSRFEDQPGRAPGLIAVADAYAMSGVERLAPRLLTDL
                     WMSGVEEWEAEEVLADADVEKGDVLPCRSCWARYVCTHSSLASSGLEGDDAREPSEVR
                     CGIWKDEVEASLRLYHRMAHADPIQVLRLFEEATTVPGEPLAGWSGDLIKPS"
     gene            complement(980..2956)
                     /locus_tag="VEL74_09760"
     CDS             complement(980..2956)
                     /locus_tag="VEL74_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002694751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HYG62855.1"
                     /translation="MKRFEVIRMRRSTLPLSSLLPVLLLAACSGQPQPMDPAPAPAAA
                     PAAAAPAATQTAPAAPAAAPDRDPHSFSRPDEVVVQHLKLDLNVDFSRRQLSGRASLR
                     LDNRSGANRLVLDTRDLEIRQVLLDSGTAARYTLGEEVEFLGRPLEVEITPRTQWVHV
                     DYSTRPEAAALQWLDPQQTAGGRHPFLLTQSQAILARTWIPLQDTPGVRMTYEATVRV
                     PQPQGLLALMSAENPTAKRADGTYTFKMPQPIPSYLMALAVGDLEFRPLGPNSGVYAE
                     PPVIELAAWELADTPRMITTAERLFGPYRWGRYDMLVLPPSFPYGGMENPRLTFLTPT
                     MLARDRSLVSLIAHELAHSWSGNLVTNATWNDFWLNEGFTTYAEGRIMEALYGNEYAD
                     MLAVLGRQDLDDAFEEMGGPQSPDTHLFLALAGRDPDEGTSNVAYEKGALFLRMLEQA
                     VGREAFDRFLRGYFDSHAFQSIDTTRFVALLKRDLLGGNAQRYASLRIDQWIYGPGVP
                     DNAPQPRSTAFAAVDAAVAAFAKGTPAAQLGTQGWTTHHWLHFLRTLPKPLSREQMAD
                     LDAAFKLSQSGNSEILTEWLVRVVENRYDPVYPVLERFLTSVGRTKFLRPIYTAMAKT
                     PAGADMALRIYEKARPGYHAVSRAALEKILDWQG"
     gene            complement(2997..>3784)
                     /locus_tag="VEL74_09765"
     CDS             complement(2997..>3784)
                     /locus_tag="VEL74_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HYG62856.1"
                     /translation="EALERFARLLERYEPREVEEVSRLWAQEVPCEQTGYRELLAELP
                     AVLAAFFALSPNAVELIREHVLRTVHGMAGFVARMDGGELRLRDIPDLQVYCYVVAGI
                     VGELLTELFLRERPGLAPAAGRLRERARSFGEGLQLVNILKDSASDAVQGRIYLPEGV
                     ERTEVLALARRNLETAAEYVHTLQEAGAERGVVAFTALPVRLAHATLDRVEASGPGSK
                     LTRHEVYAILQRLHQALDRNEPALSSVSGGSAAEPGVPFVPGL"
BASE COUNT          536 a         1307 c         1413 g          528 t
ORIGIN      
        1 gcgcccgcca cctggacgcg gtgcgcgtcg agtcctcgac cgccgacggg gtggacgcgc
       61 tggtctggac gcggcagttc cgcgaggatc tgcaggtgat ggcggacgag atgggcaacg
      121 acctggaagc gcaggagatg cccctggact tcctgcccat gacccggatc gtccggcgcc
      181 tgatgcggag cgagccgatg tcgcgcttcg aggaccagcc cggccgcgcc cccggtctga
      241 tcgcggtggc cgacgcctac gccatgtccg gcgtggagcg cttggccccc cggctgttga
      301 ccgacctctg gatgagcggc gtcgaggagt gggaggcgga ggaggtgctc gccgacgccg
      361 acgtcgagaa gggggacgtc ctgccgtgcc ggagctgttg ggcccgctac gtgtgcaccc
      421 acagctcgct cgcctcctcg ggcctcgagg gggacgacgc gcgagagccg tcggaggtcc
      481 gctgcggcat ctggaaggac gaggtggagg cctccctgcg cctctaccac cggatggcgc
      541 acgccgatcc catccaggtc ctgcgcctgt tcgaggaggc caccaccgtc ccgggcgagc
      601 ccctggccgg ctggtccggc gacctgatca agccctcctg agcgatcagg ggggcgggcg
      661 ccttccgccc ccccgaattc cacccgaacg tcccggatct ctcccggagg ccgcggacgc
      721 gatccgaaag ctccgggaag cttcccggaa gccccggatc gcatccgggg cacgcggatg
      781 tcatccagag ggtccgggag cttcccgaac gtcccggatc acgtccagct catccggatg
      841 gagtccggcc gcaccggatc gcgtccaatt cattcggatg gattccaggc cgcccgggaa
      901 gctcccgagc gctccggatc gcatcccgtg gaggcggatg gagcccgaag cgccggggct
      961 ggcgccgtgg agtccggccc tacccctgcc agtccaggat cttctccagc gcggcgcggg
     1021 agaccgcgtg gtagcccgga cgcgccttct cgtagatgcg gagcgccatg tcggcgccgg
     1081 ccggggtctt ggccatcgcg gtgtagatcg gccgcaggaa cttcgtccgg ccgacgctgg
     1141 tcaggaacct ctccagcacc ggatagaccg ggtcgtagcg gttctccacc acccgcacca
     1201 gccactcggt caggatctcg gagttgcccg actggctcag cttgaacgcc gcgtccaggt
     1261 ccgccatctg ctcccgggag agcggcttgg ggagggtgcg caggaagtgc agccagtggt
     1321 gggtggtcca gccctgggtg ccgagctgcg cggcgggcgt ccccttggcg aaggcggcga
     1381 cggcggcgtc caccgcggcg aaggctgtgg agcgcggctg cggcgcgttg tccggcacgc
     1441 ccgggccgta gatccactga tcgatccgca gcgacgcgta gcgctgggcg ttgcctccca
     1501 gcaggtcgcg cttgagcagg gcgacgaagc gggtggtgtc gatggactgg aaggcgtggc
     1561 tgtcgaagta gccgcgcagg aagcggtcga acgcctcgcg gcccacggcc tgctccagca
     1621 tgcgcaggaa gagcgccccc ttctcgtagg cgacgttgct cgtcccctcg tcggggtcgc
     1681 ggccggcgag ggccaggaag agatgggtgt cgggggactg cggcccgccc atctcctcga
     1741 acgcgtcgtc caggtcctgg cggccgagca cggcgagcat gtccgcgtac tcgttgccgt
     1801 agagggcctc catgatccgc ccctcggcgt aggtggtgaa cccctcgttc agccagaagt
     1861 cgttccaggt ggcgttggtc accaggttcc ccgaccagga gtgcgccagc tcgtgcgcga
     1921 tgagcgacac cagcgaccgg tcgcgcgcca gcatggtggg ggtgaggaag gtcagccgcg
     1981 ggttctccat gccgccgtag gggaagctcg gcggcagcac cagcatgtcg tagcgccccc
     2041 agcggtaggg gccgaagagc cgctcggcgg tggtgatcat gcgcggcgtg tcggccagct
     2101 cccaggcggc cagctcgatc accggcggct cggcatagac gccgctgttc ggccccagcg
     2161 gccggaactc caggtcgccc acggccagcg ccatgagata cgacgggatc ggctgcggca
     2221 tcttgaaggt gtaggtgccg tcggcccgct tcgcggtcgg gttctcggcg ctcatcagcg
     2281 ccagcaggcc ctgcggctgc ggcacccgga cggtcgcctc gtaggtcatg cgcacgcccg
     2341 gcgtgtcctg gagcgggatc caggtgcggg cgaggatggc ctgcgactgg gtgaggagga
     2401 acgggtgcct gccgcccgcc gtctgctgcg ggtcgagcca ctgcagcgcg gccgcctccg
     2461 gccgggtgga gtagtccacg tgcacccatt gcgtccgcgg cgtgatctcc acctccagcg
     2521 gcctgcccag gaactccacc tcctcgccca gcgtgtagcg ggccgccgtg ccgctgtcca
     2581 gcagcacctg ccggatctcc aggtcgcggg tgtcgaggac cagccggttt gccccggagc
     2641 ggttgtccag gcgcaggctc gcccggcccg agagctggcg gcggctgaag tccacgttca
     2701 ggtcgagctt cagatgctgg accacgacct cgtcgggacg ggagaaggag tgcgggtccc
     2761 ggtcgggagc cgcggccggc gccgccggtg ccgtctgcgt ggccgcggga gccgccgccg
     2821 ccggagccgc ggctggagcg ggagcggggt ccatgggctg gggctggccg gagcaggccg
     2881 ccagcagcag aacggggagg agcgacgaaa ggggcagagt ggaacgcctc atgcggatca
     2941 cctcgaatct cttcatcctc cccttcaacc gggaggcggg tgcgcgactt cccggctcag
     3001 agcccgggaa cgaacgggac gccgggctcg gccgcggagc cgccggagac actggagagc
     3061 gccggctcgt tgcggtcgag cgcctggtgg aggcgctgga ggatggcgta gacctcgtgg
     3121 cgcgtcagct tggagcctgg accggaggcc tccacccggt ccagggtggc gtgggcgagc
     3181 cggacgggca gcgcggtgaa cgccaccacc ccgcgctccg cgccggcctc ctgcagggtg
     3241 tgcacgtact ccgccgcggt ctccaggttg cgccgggcga gcgccaggac ctcggtgcgc
     3301 tccaccccct cgggcaggta gatccgcccc tggaccgcgt ccgacgccga atccttgaga
     3361 atgttgacga gctgcagccc ctcgccgaag gagcgggcgc gctctcgcag ccgcccggcg
     3421 gccggcgcga gaccggggcg ctcgcgcagg aagagctcgg tcagcagctc gccgacgatg
     3481 ccggcgacga cgtagcaata gacctggagg tcggggatgt cgcgcagccg cagctcgccg
     3541 ccgtccatgc gggcgacgaa cccggccatg ccgtggacgg tgcgcagcac gtgctcgcgg
     3601 atcagctcga cggcgttggg ggacagggcg aagaaggcgg cgagcaccgc gggcagctcg
     3661 gcgaggagct cgcggtagcc cgtctgctcg cacggcacct cctgcgccca gaggcgcgac
     3721 acctcctcga cctcccgggg ctcgtaccgc tccaggagcc gggcgaagcg ctccagcgcc
     3781 tcga
//
LOCUS       DASPJI010000365         7737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_831013, whole genome shotgun sequence.
ACCESSION   DASPJI010000365 DASPJI010000000
VERSION     DASPJI010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7737)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831013"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VEL74_09770"
     CDS             complement(<1..402)
                     /locus_tag="VEL74_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62857.1"
                     /translation="MRAVKLFSFCALALCAVPLTAQTRQAAGLSTPRSGLPTLQAQDS
                     PLMEEALASRFLFFALTPEQAAGTPESLGFRIDLNGQPFVEETLDLRQVPDAQSAAFE
                     ILATRPDLRDRLYELGRKGSNRLTVQVVVDGA"
     gene            complement(566..1534)
                     /locus_tag="VEL74_09775"
     CDS             complement(566..1534)
                     /locus_tag="VEL74_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62858.1"
                     /translation="MKRALKLWSCSCVLTLCALPLVAQPRTPSLPADRAVPAPRGVVD
                     SRFLFFALTPELAAGTPESLAFRIDVDGQPFLVETLDLRQVRGAESFAFELLATRPDL
                     RDRLHELGRDPKSRVVVQVAVDGAVVREFASFGELLRYNREIKRFLRPAAAPSEVYGP
                     AAGESPKPAPLARLFEKGYQADPACLSQCQYEYQYCISGPCYEAGASCVFCEDLYQSC
                     LESCPIICVEPKSVTEYYTPWQTISTIYGSYNCYENWWENDFLWGTYYQHVSYSLKRD
                     RVRRTESCNGQVTETVLSTQYSNSSCWQPTGASCYMPPSRFSGWSC"
     gene            complement(1649..1723)
                     /locus_tag="VEL74_09780"
     tRNA            complement(1649..1723)
                     /locus_tag="VEL74_09780"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1689..1691),aa:Asn,seq:gtt)
     gene            complement(1804..3516)
                     /gene="rpoD"
                     /locus_tag="VEL74_09785"
     CDS             complement(1804..3516)
                     /gene="rpoD"
                     /locus_tag="VEL74_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HYG62859.1"
                     /translation="MTISSLAPVEEKYSSVKQLIELGKEKGYLLYDEIYEMLPEEVVS
                     LPDELDGIYIRFADLGIDVIDRPERYQNRDDFESSGASEFDKKEDETTEFGLAAHEKT
                     NDPVRMYLREMGTVPLLDREGEVEIAQRIEQGEWMIYEALCENPVVLRELLRLNEMAA
                     KDKRILRELLAADPDEPLDPKATDRIRGNLKIFEQIAANDQEIQELRKRQKRCKPGGD
                     KYLEFEREVDRLVAKIAKDIRTIDFSVQTRNRLVEFLRDIDKQFSRYEQDIKRAQTAL
                     KRESNEELKALHRRRIDKYRGHLRELEERYGTTHAEIAATIGKIHQGEALCERAKEEL
                     IVANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVEKFEYRRGYKFSTYATWWIRQ
                     AITRAIADQARTIRIPVHMIETINKLTRTSRSLVQELGREPTAEEIGERMDLPANKVR
                     KIMKIAQEPISLETPIGEEEDSHLGDFIEDKNAVSPIDSVIFGNLKDQTRKVLKTLTP
                     REELVLKMRFGVGDGSEHTLEEVGRSFNVTRERIRQIESKALRKLRHPSRATKLKPFL
                     DNTV"
     gene            complement(3574..5436)
                     /gene="dnaG"
                     /locus_tag="VEL74_09790"
     CDS             complement(3574..5436)
                     /gene="dnaG"
                     /locus_tag="VEL74_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HYG62860.1"
                     /translation="MALGNIHLTPQLVQAVRDAVDILAIASEHTRLRKAGRRYQGLCP
                     IHKEKTPSFSVDPVQGLFYCFGCGSGGDAIKLHMLATGDDFPGAIEALATRYGVPLPT
                     RNEARFAGGRPERDIEGALRAAAEFFTDQLRKAPLPQGYLAKRRIPGELIERFGLGYA
                     PEGWRNLVQALHPRISLADLEAAGLVARPERGGDPYDRFRNRLMFPIRTASGRLVGFG
                     GRTLGDDAAKYINTNETEQFHKGNLLYGLDLAKREVRESGRAVLVEGYFDVIGAVACG
                     LEGAVAGMGTALTPEQAKLLSRYAEEVVVAYDGDNAGENAFRRALPLLLAEGLGARRA
                     RFGEGHDPDSLRLEQGEAAVAAAVDAAEDAVVAELHRLIPPEAPREPPVQAKAASAVA
                     DLLRPIPDAVLRFGYARLGAERLGLPVEMLGRRIGGQGGQQAGGGRDGRDGRREGDRS
                     AAQPGGPSPQARRPVPRAPRETRNVEEQVLQYLLQGEGTVPPLEELPSPGWFFDTECR
                     NIYQAYSDLYARGAGAPPDARAVLAELGSQGATVDRVAEILLEESFDPRTHGLRESLE
                     KLKRRWIGQRRQEIQRRIHEAVRSGDEALANRLTDEKRDLDRQYYGATRPGAGG"
     gene            complement(5495..>7737)
                     /locus_tag="VEL74_09795"
     CDS             complement(5495..>7737)
                     /locus_tag="VEL74_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HYG62861.1"
                     /translation="TGRPPLEGLDLVRLADLGKACRTVAARIQEADPPCPALGALVAG
                     LPDISPLLHRIDRALDRRGEVREDASPRLAALRQQIRRVRDQIYRELGSYAEGHREDL
                     AEDTVPLRGGRLVVMLQAGAKGRSGGLVHGRSATGKSLYFEPLEVVETNNTLQQSVED
                     EEAERRRILAELMGEARAALPALETHAHLFAELDLLQSMVRFGDLCGAALPDIGPRFD
                     LRLLSARHPLLDPGLAELREEALGHAGHTGTIVPLDIELTSERRALVVTGPNAGGKTV
                     ALKTTGLLTLAAQCGLPVPAAAGSRVPFVTRLVATVGDEQDLLADRSTFSGRLLRLKE
                     AWEAAGPDSLVLLDELGSGTDPEEGAALSIALLEGLLTKGSFAVITTHLAQLAAAALE
                     MGGASCAAMEFDPETGRPTFRLLPGPPGGSEALALARRLGLPAEWLDRAEARLGSEHR
                     DLRRLLAEVERVRRELAETQERLEIEASDAEKLRRRLADERAALEEERRSVGRRLRAE
                     LDGFRRETGDRLRQEVERLRESFEEGRRRNLAAEAVERLFAEAPVLGEEEPEPAGPVS
                     VGQPVRHVGLGWEGVLEKLEAGRAEVLVRGKRVRCRPEELAPARPGAAGGSAVNRATP
                     STRAREGKPAIAGANRTGQDRTAQDLPGRDRPRGDLEEAGVPKELNLIGQRVEAALEE
                     LDAYLDQALLASRKEVRVIHGHGTGRLRQAVREHLRGHPGVTAQRPGAPDEGGNGATV
                     VVLRGA"
BASE COUNT         1060 a         2640 c         2857 g         1180 t
ORIGIN      
        1 ggcgccatcg acgacgacct ggaccgtcag ccggttcgag cctttgcggc ccagctcata
       61 aagcctgtcg cgcaggtcgg ggcgggtggc gaggatctcg aaggcggcgg attgcgcgtc
      121 cggcacctgc cgcaggtcca gggtctcctc gacgaagggc tggccgttca ggtcgatgcg
      181 gaaacccagg gactccgggg ttccggccgc ctgctccggg gtgagggcga agaagaggaa
      241 acggctcgcc agcgcctcct ccatcaacgg agagtcctgc gcctggagag tgggcaggcc
      301 ggagcggggg gtcgagagac ccgcggcctg ccgggtctgg gcggtcaagg gaacggcgca
      361 cagcgccagg gcacagaaag agaaaagctt cacagctcgc atcgagtggc tcctgttgaa
      421 gggggcggag aggaatcctc cgccgcttgc accagataac gtcagcgggt gcgcaagaac
      481 ctcaccccgg cgcgaggcct gaagcgagga gggccacccg cgtgcgggtg gccccctcgt
      541 ggaccgtgat gctcccgcgc ggggatcagc aggaccaacc cgagaagcgc gagggcggca
      601 tgtagcagct cgcccccgtg ggctgccagc aggagctgtt cgagtactgg gtactgagca
      661 ccgtctccgt cacctgaccg ttgcaggact cggtcctgcg cactctgtcc cgcttgaggg
      721 agtagctcac gtgctggtag taggtgcccc agaggaagtc gttctcccac cagttctcgt
      781 agcagttgta ggagccgtag atggtgctga tggtctgcca gggcgtgtag tactcggtga
      841 cgctcttcgg ctccacgcag atgatcgggc aggactccag gcaagactgg tagaggtcct
      901 cacagaagac gcagctcgcc ccggcctcat agcagggtcc gctgatgcag tactgatact
      961 cgtactggca ctgcgagaga caggcggggt cggcttgata gcccttctcg aagagacggg
     1021 cgagaggcgc gggcttcggg gactcgccgg cggccgggcc gtagacctcg gaaggcgcgg
     1081 cggccgggcg gaggaagcgc ttgatctcac ggttgtagcg cagcagctcc ccgaacgagg
     1141 cgaactcgcg gaccacggcg ccgtcgacgg cgacctggac gaccacgcgg ctcttcgggt
     1201 cgcggcccag ctcgtggagc cggtcgcgca ggtccggccg ggtggcaagg agctcgaagg
     1261 cgaacgactc ggcgccgcgg acctgccgca ggtcgagggt ctcgacgagg aacggctggc
     1321 cgtccacatc gatccggaag gccagggact ccggcgtccc cgccgccagc tcgggggtca
     1381 gggcgaagaa gaggaagcgg ctgtcgacca cgccgcgggg agcgggaacc gcacggtccg
     1441 cggggaggga cggggtacgg ggctgcgcca ccaggggaag agcgcagagc gtcaggacac
     1501 agctgcagga ccagagcttc aaggcacgtt tcatggttct cctcctgaag acgggcgtca
     1561 agcgacgtct tatacatcga caggtgtgca tgaaacctgg aggcgagccg gagccggccg
     1621 ggaacgttgc gaagggaaag ggtaggaatg gctcctgggg agggatttga acccccgacc
     1681 aatcggttaa cagccgatcg ctctaccgct gagctaccca ggagtgtgaa cgaacacgcc
     1741 tccggagggt tgcccctccg aaggccggaa tcttaggcgg atcgcgccgc cctgtcaatt
     1801 cctctagacg gtgttgtcga ggaacggctt cagcttggtc gcccggctgg ggtgacggag
     1861 cttgcgcagc gccttgctct cgatctggcg gatgcgctcg cgcgtcacgt tgaacgagcg
     1921 gccgacctcc tccagggtgt gctcggagcc gtcgccgacg ccgaagcgca tcttgagcac
     1981 cagctcctcg cgcggcgtca gggtcttcaa caccttgcgg gtctggtcct tgaggttgcc
     2041 gaagatcacc gagtcgatgg gggagacggc gttcttgtcc tcgatgaaat ccccgaggtg
     2101 cgaatcctcc tcctcaccga tgggcgtctc gagcgagatg ggctcctgcg cgatcttcat
     2161 gatcttgcgc accttgttgg ccggcaggtc catgcgttcg ccgatctcct cggcggtggg
     2221 ctcgcggccc agctcctgca ccagcgagcg gctggtgcgg gtgagcttgt tgatggtctc
     2281 gatcatgtgc accgggatgc ggatcgtccg ggcctggtcc gcgatggcgc gggtgatggc
     2341 ctggcggatc caccaggtgg cataggtcga gaacttgtag ccgcggcggt actcgaactt
     2401 ctccaccgcc ttcatcaggc cgatgttccc ctcctggatc aggtcgagga actggaggcc
     2461 ccggttggtg tacttcttgg cgatcgacac cacgagccgc aggttggcga cgatcagctc
     2521 ctccttggcg cgctcgcaga gcgcctcgcc ctggtggatc ttgccgatgg tcgcggcgat
     2581 ctcggcgtgc gtcgtgccgt agcgctcctc gagctcccgc aggtggccgc ggtatttgtc
     2641 gatccgccgc cggtgcaggg ccttgagctc ctcgttcgac tcgcgcttca acgccgtctg
     2701 cgcccgcttg atgtcctgct cgtagcggga gaactgcttg tcgatgtcgc gcaggaactc
     2761 caccaggcgg ttgcgcgtct gcacagaaaa gtcgatggtg cggatgtcct tcgcgatctt
     2821 ggccaccagc cggtcgacct cgcgctcgaa ctccaggtac ttgtcgcccc ccggcttgca
     2881 gcgcttctgg cgcttgcgca gctcttggat ctcctggtcg ttggcggcga tctgctcgaa
     2941 gatcttgagg ttgccacgga tgcggtcggt cgccttgggg tcgagcggct cgtcgggatc
     3001 ggcggcgagg agctcgcgca ggatgcgctt gtccttggcc gccatctcgt tgaggcgcag
     3061 gagctcccgc agcaccaccg ggttctcgca cagcgcctcg tagatcatcc actcgccctg
     3121 ctcgatgcgc tgggcgatct ccacctcgcc ctcgcggtcg aggaggggga cggtgcccat
     3181 ctcgcgcagg tacatgcgga cggggtcgtt ggtcttctcg tgggcggcga gaccgaactc
     3241 cgtggtctcg tcctccttct tgtcgaactc gctcgcgccg gaggactcga agtcgtcccg
     3301 gttctggtac cgctcgggac ggtcgatgac gtcgatgccg aggtcggcga agcggatgta
     3361 gatcccgtcg agctcgtccg ggaggctcac cacctcctcg ggcagcatct cgtagatctc
     3421 atcgtagaga aggtagccct tctccttacc cagctcgatc aactgcttga ccgaggagta
     3481 cttctcttcg acgggtgcca ggctggaaat ggtcaatgtg ctctcctaac ggcttgcacc
     3541 gaagacccgg tgaggacaca cacctccacc gggtcatccg cctgcgccag gacgggttgc
     3601 cccgtagtac tggcggtcta gatctctctt ctcatccgtg agacgattgg cgagcgcctc
     3661 gtcccccgag cgtacggcct cgtggatcct tcgctggatc tcctggcggc gctgtccgat
     3721 ccatcgccgc ttcagctttt cgagggactc ccggagcccg tgcgtgcgcg ggtcaaacga
     3781 ctcttctaac agaatctcgg ccacccggtc aactgtcgcc ccctgggagc ccagctcggc
     3841 gaggaccgcc cgtgcgtccg gaggggcgcc tgcccctctt gcatatagat cggaataggc
     3901 ctgataaata ttcctgcatt ccgtatcgaa gaaccagccg gggctcggca gctcttccag
     3961 ggggggcacg gtcccctctc cctgtaacaa gtactgcagc acctgctcct cgacgttgcg
     4021 ggtctcccgc ggcgcccgcg ggactgggcg gcgcgcctga ggggaggggc cgcccggctg
     4081 ggctgcggag cggtcgccct ccctgcgccc atcccgcccg tcgcgcccgc cgccggcctg
     4141 ctggcctccc tggccgccga tacgccggcc cagcatctcg accggcaggc cgagccgctc
     4201 cgcccccagc cgcgcatagc cgaaccgcag caccgcgtcg gggatcggcc gcagcaggtc
     4261 ggcgaccgcc gaggcggcct tcgcctggac cggcggctcg cgcggcgcct ccggggggat
     4321 caggcggtgg agctcggcga ccaccgcgtc ctcggcggcg tcgacggcgg cggccaccgc
     4381 cgcctccccc tgctccaggc gcagggagtc ggggtcgtgc ccctcgccga agcgggcgcg
     4441 gcgcgccccc agcccctcgg cgaggagcag cggcagggcg cgccggaagg cgttctcccc
     4501 cgcgttgtcg ccgtcgtagg cgaccaccac ctcctcggcg tagcgggaga ggagcttcgc
     4561 ctgctcgggg gtcagcgcgg tgcccatgcc cgccaccgcc ccctccaggc cgcaggcgac
     4621 ggcgccgatg acgtcgaagt agccctcgac cagcacggcg cgcccgctct cgcggacctc
     4681 gcgcttcgcc aggtcgagcc cgtagagcag gttgcccttg tggaactgct cggtctcgtt
     4741 ggtgttgatg tacttggcgg cgtcgtcgcc cagggtgcgg ccgccgaagc cgaccaggcg
     4801 gcccgaggcg gtgcggatgg ggaacatcag ccggttgcgg aagcggtcgt aggggtcgcc
     4861 gccgcgctcc ggccgggcga ccaggccggc ggcctcgagg tcggccaggg agatgcgcgg
     4921 gtggagcgcc tgcaccaggt tgcgccagcc ctccggggcg tagccgaggc cgaagcgctc
     4981 gatcagctcg ccggggatgc ggcgcttcgc caggtagccc tgggggagcg gcgccttgcg
     5041 gagctggtcg gtgaagaact cggcggcggc gcgcagcgcc ccctcgatgt cgcgctccgg
     5101 ccggccgccg gcgaagcgcg cctcgttgcg ggtgggcagc ggcacgccgt agcgggtggc
     5161 gagggcctcg atggcgccgg ggaagtcgtc gccggtggcc agcatgtgca gcttgatggc
     5221 gtcgccgccg gagccgcagc cgaagcaata gaagagcccc tgcaccggat cgacgctgaa
     5281 cgagggggtc ttctccttgt ggatcgggca cagcccctgg tagcgccgac cggccttgcg
     5341 caggcgggtg tgctcggagg cgatggcgag gatgtcgacc gcgtcgcgca ccgcctgcac
     5401 gagctgcggg gtgagatgga tgttgcccag ggccatggcg gcggcgatag cggcgatgtc
     5461 ggcgatagcg gcgatgtcgg gcgcggtgcg gcggtcaggc gccgcggagg acgaccacgg
     5521 tggcgccgtt gcccccctcg tcgggggccc ccggccgctg ggcggtgacc cccgggtggc
     5581 cgcgcaggtg ctcgcgcacc gcctgccgca gccggccggt gccgtggccg tggatgaccc
     5641 gcacctcctt gcgcgaggcg aggagggcct ggtcgaggta ggcgtccagc tcctcgagcg
     5701 ccgcctcgac ccgctggccg atcaggttca gctccttcgg cacgccggcc tcttccaggt
     5761 ccccgcgcgg gcggtcccgt ccaggcaggt cctgggccgt ccggtcctgc ccggtccggt
     5821 tggcgccggc gatggccggc ttcccctccc gcgcgcgggt ggacggggtg gcccggttga
     5881 cggcactgcc gccggcagcg ccgggccggg cgggggcgag ctcctcgggg cggcagcgca
     5941 cccgcttgcc gcgcaccagg acctcggcgc ggccggcctc cagcttctcc agcaccccct
     6001 cccagccgag gccgacgtgg cgcaccggct ggccgacgct gacggggccg gcgggctccg
     6061 gctcctcctc gcccaggacc ggggcttcgg cgaacaggcg ctcgaccgcc tcggcggcga
     6121 ggttgcggcg ccgcccctcc tcgaagctct cgcgcagccg ctcgacctcc tggcgcaggc
     6181 ggtcgccggt ctcgcgccgg aagccgtcga gctcggcgcg cagccggcgc cccaccgagc
     6241 gccgctcctc ttccagggcg gcgcgctcgt cggcgaggcg gcggcggagc ttctcggcgt
     6301 cggaggcctc gatctccagc cgctcctggg tctccgccag ctcgcggcgc acccgctcca
     6361 cctcggcgag gaggcggcgc aggtcgcggt gctcggagcc gaggcgggcc tcggcgcggt
     6421 cgagccactc ggcgggcagg ccgaggcggc gggcgagggc cagggcctcg ctccctcccg
     6481 gcggcccggg cagcagccgg aacgtcggcc ggccggtctc cgggtcgaac tccatggcgg
     6541 cgcacgaggc gccgcccatc tccagggcgg cggcggcgag ctgcgccagg tgcgtggtga
     6601 tgacggcgaa gctccccttg gtgaggagcc cctcgagcag ggcgatggag agggcggccc
     6661 cctcctccgg gtcggtgccc gagcccagct cgtcgagcag caccaggctg tccggcccgg
     6721 cggcctccca ggcctccttg aggcgcagca ggcggccgct gaaggtggag cggtcggcca
     6781 gcaggtcctg ctcgtcgccg acggtggcca ccaggcgggt gacgaacggc acccggctgc
     6841 ccgcggcggc ggggaccggc aggccgcact gcgccgccag ggtgaggagg ccggtggtct
     6901 tgagggcgac ggtcttgccg ccggcgttgg ggccggtgac gaccagggcc cgccgctcgg
     6961 aggtgagctc gatgtccagc ggcacgatgg tgccggtgtg cccggcatgg cccagcgcct
     7021 cctcgcgcag ctcggcgagg ccggggtcga gaagcgggtg gcgggcggac agcagccgca
     7081 ggtcgaagcg cgggccgatg tccggcaggg cggcgccgca gaggtcgccg aagcggacca
     7141 tggactgcag caggtcgagc tcggcgaaca ggtgggcgtg ggtctcgagg gcggggaggg
     7201 ccgcccgcgc ctcgcccatc agctcggcga ggatgcggcg ccgctcggcc tcctcgtcct
     7261 ccaccgactg ctgcagcgtg ttgttggtct ccaccacctc gagcggctcg aagtagaggc
     7321 tcttgccggt ggccgatcgg ccgtgcacca ggccgccgga gcgccccttg gcgccggcct
     7381 ggagcatcac caccagccgg ccgccacgca gggggacggt gtcctcggcc agatcctcgc
     7441 ggtgcccttc ggcgtaggag ccgagctcgc ggtagatctg gtcgcgcacc cggcggatct
     7501 gctggcgcag ggcggcgagg cgggggctcg cgtcctcccg cacctcgccg cggcggtcga
     7561 gggcgcggtc gatacggtgg agcagggggg agatatcggg cagcccggcc accagcgcgc
     7621 cgagggcggg gcagggcggg tcggcctcct ggatgcgcgc cgcgacggta cggcaggcct
     7681 tgcccaggtc ggccaggcgc acgaggtcca gcccctcgag cggcgggcgg ccggtga
//
LOCUS       DASPJI010000366         3375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_655526, whole genome shotgun sequence.
ACCESSION   DASPJI010000366 DASPJI010000000
VERSION     DASPJI010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655526"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3375)
                     /locus_tag="VEL74_09800"
     CDS             complement(<1..>3375)
                     /locus_tag="VEL74_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62862.1"
                     /translation="HSLLATQVVSRVRQAFGVELPIRDLFEEPTVAGLAGRLGRLGRT
                     EVEAPSIVPVPRQADLPLSFAQQRLWFLHRLAPDSAVYNVPLAVRLSGRLDAAALAGA
                     LRGIGRRHEALRTTFDERQGHPVQVIEPEPRLTLTLLDLSDLPPAEREAEVLRQAHTE
                     ALAPFDPAAGPLVRATLLVLGEEEHTLLVTMHHIVTDGWSMGIFLRELTALYAGRELP
                     ELPVQYGDFAAWQRAWLSGEVLESQISYWRERLAGAPVRLTLPTDRPRPAVETHRGAS
                     LPVTLPPELSQSLTALARREGATPFMILMAGFAVLLGRYSGQGDVVVGAPIAGRNRRE
                     IEGLIGFFVNTLALRVELADEQGFLDLVRQVRRTALDAYAHQDIPFERLVEELVTERD
                     LSHSPLFQVVLTLQSASVSSEGGGVTGLELRPVEVESGTAKFELSLGFAQREEELAGS
                     LEYNTDLFDTATAERLARHLARLLEGAAGSPGLRLAELPLLDESERQQLLQEWGQGLA
                     TPANDQPVHRLVAARAAETPDAVALVSAAGHAVTYGELSRRAARLARRLRSLGAGPEE
                     RVGVLLDRSPEEVTSLLAVLTAGGAYVPLDPAWPRERVELVLADAGVRALLTTSDRSD
                     LPGPILLVDRVWDEPYPGSSEASVDVNHLAYVIYTSGSTGRPKGVEITHRGLLRLLAW
                     HRRAFGVTAADRATRLSGLAFDAAVWELWPYLAAGASVHVPDEETRSSPPALRDWLLG
                     EEITIGFVSTPVAEALLPLPWPASTSLRVLLTGGDRLHRDPGPLPFTLVNNYGPTEST
                     VVATSGPVQARGPGRAPDIGQPIDGTRVLLLERELRLVPPGVAGEICIGGPGLARGYL
                     GQPGLTAERFLPDPFTTDPGERLYRTGDLARHLPDGTIDFLGRIDFQVKIRGLRIELG
                     EIEAALAAHPQVRDAAVLVREDRSGQPGLVVYLAAHSDAGPEELRRWLRGRLPEAMVP
                     PSFVLLESLPLTPNGKLDRKALAGIGPAREEGGGPETYVAPRTPAEEVLAGIFAEVLG
                     QERVGIHDGFFELGGHSLLATQVVSRVRQAFGVELAIRELFEAPTVARLAERIERIGT
                     STVEAPPIVPVPRDRDLPPSFAQQRLWFVDRLKPGSS"
BASE COUNT          512 a         1318 c         1132 g          413 t
ORIGIN      
        1 ggagctgccc ggcttgagcc ggtccacgaa ccagaggcgc tgctgggcaa acgacggcgg
       61 caggtctcga tcccgcggca ccggcacgat cggcggcgcc tccacggtgg aggtcccgat
      121 ccgctcgatc ctctcggcca gccgcgccac cgtgggtgcc tcgaacagct cacggatcgc
      181 cagctccacc ccgaaggcct ggcgcacccg cgacaccacc tgggtggcca gcagcgagtg
      241 cccgccgagc tcgaagaagc cgtcgtggat cccgacccgc tcctgcccca gcacctccgc
      301 gaagatcccc gccagcacct cctccgccgg ggtgcgcggt gctacgtacg tctccggccc
      361 gccaccctcc tctcgggccg gcccgatccc ggccagcgcc ttgcggtcca gctttccgtt
      421 gggggtcagc ggcagggact ccagcagcac gaacgacgga ggcaccatcg cctcggggag
      481 ccggccgcgc agccagcgcc gcagctcctc cggtcccgcg tcgctgtgcg ccgccaggta
      541 gaccaccagg cccggctgtc cggagcggtc ttcccgcacc aggaccgctg catcccgcac
      601 ctgcgggtgg gccgccagcg ccgcctcgat ctcacccagc tcgatccgca gcccgcggat
      661 cttgacctgg aagtcgatcc gccccaggaa gtcgatggtt ccgtcgggca ggtggcgcgc
      721 caggtctccc gtgcggtaca gcctctcgcc gggatcggtc gtgaaggggt ccggcaggaa
      781 gcgttcggcg gtgaggcccg gctgccccag gtagccccgg gccaggcccg ggccgccgat
      841 gcagatctcc ccggccaccc caggcggcac cagacgcagc tcccgttcca gcagcagcac
      901 ccgggtgccg tcgatcggct ggccgatgtc tggcgcgcgg ccagggcccc gtgcctggac
      961 cggacccgag gtcgcgacca ccgtgctctc cgtcggaccg tagttgttga ccagcgtgaa
     1021 cggcagcggg cccggatcgc ggtgcaggcg gtcgccgccg gtcagtagca cccgcagaga
     1081 ggtggaagcg ggccagggaa gtggcaacag cgcctcggcc accggcgtcg agacgaagcc
     1141 gatggtgatc tcctcgccga gcagccagtc gcgcagggcc ggcggcgagc tgcgggtctc
     1201 ctcatccggc acgtggacgc tggcgccggc ggcgagatag ggccacagct cccagaccgc
     1261 ggcatcgaag gccaggcccg acagccgggt cgcgcggtcc gccgccgtca cgccgaaggc
     1321 ccgccggtgc caggccagca ggcgcagcag gccgcggtgg gtgatctcca cccccttggg
     1381 acggcccgtc gagcccgagg tatagatgac ataggccaag tggttgacat cgacggaggc
     1441 ctcggagctc cccgggtacg gctcgtccca gacgcggtcc accagcagga ttggccccgg
     1501 caggtcgctc cgatccgagg tggtcagcaa cgcccggacg cccgcgtccg cgagcaccaa
     1561 ctccacccgc tcgcgtggcc aggccggatc gagcggcaca taggcgcccc cggctgtgag
     1621 caccgcgagc aggctcgtga cctcctccgg cgagcggtcc agaaggactc ccacccgctc
     1681 ctccgggccc gctcccaagg agcgcagacg gcgggcgaga cgcgccgcgc gccggctcaa
     1741 ctcgccgtag gtaacggcat ggccggccgc cgagaccagc gccaccgcgt ccggcgtctc
     1801 ggctgcccgc gcggcgacca gccggtgcac cggctggtcg ttcgcgggcg tcgccagccc
     1861 ctgcccccac tcctgaagga gctgctgccg ttcggactca tccagcaatg gcagctccgc
     1921 gagccggagc cccggactgc cggcagcccc ctccagcagg cgcgccaggt gccgggcgag
     1981 acgctccgcc gtagccgtat caaacaggtc ggtgttgtac tccaggctcc ctgccaactc
     2041 ctcctcacgt tgcgcgaagc ccaggctgag ctcgaacttt gccgtccccg actccacctc
     2101 caccggccgc agctccaatc ccgtcacccc gccgccttcc gagctcacgg acgcgctctg
     2161 cagcgtcagc accacctgga acagcggtga gtggctcagg tcccgctccg tcaccagctc
     2221 ttccaccagg cgctcgaacg ggatgtcctg gtgcgcgtaa gcgtccagcg ccgtccgccg
     2281 cacctgccgc accagatcca agaagccctg ctcgtccgcc agctccacgc gcagcgccag
     2341 ggtgttgacg aagaagccga tcagcccctc gatctcccgc cggttgcggc cggcgatggg
     2401 ggcccccacc accacgtcgc cctgacccga gtagcggccc aggaggaccg cgaacccggc
     2461 catcaagatc atgaacggcg tcgccccctc gcgccgggcc agcgccgtga gcgactgcga
     2521 aagctccgga ggcagcgtca ccggcaggga ggccccacgg tgggtctcca ccgccggccg
     2581 cggccggtcc gtgggcaggg tgagacggac cggcgccccg gccagccgct cccgccagta
     2641 ggagatctgc gactccagca cctctccgga cagccaggcc cgctgccaag ccgcgaagtc
     2701 gccgtactgt accggcagct ccggcaactc gcgacccgca tacagcgccg tcagctcgcg
     2761 caggaagatc cccatggacc acccgtcggt gacgatgtgg tgcatggtca ccagcagcgt
     2821 gtgctcctct tcgcccaata ccaggagcgt cgcgcgcacc agcggcccgg ccgccgggtc
     2881 gaacggcgcc agcgcttcgg tgtgggcctg acgcagcacc tcggcctcgc gttccgccgg
     2941 tggcaggtcc gagagatcga gaagggtcag cgtcagccgc ggctccggct cgatcacctg
     3001 cacggggtga ccctggcgct cgtcgaaggt ggtgcgcagc gcctcgtgac ggcggccgat
     3061 cccgcggagc gcaccggcga gcgcggcggc atccagccgg cccgacagcc gcaccgccag
     3121 aggcacgttg tagaccgcgc tgtccggcgc cagccggtgg aggaaccaga gccgctgctg
     3181 agcaaaggac agcggcaggt cagcctgccg aggcacgggt acgatcgacg gcgcctccac
     3241 ctccgttcgc ccgagccgcc cgagccgccc ggccaggccg gccaccgtcg gttcctcgaa
     3301 caggtcgcgg atcggcagct ccacaccgaa ggcctggcgc acccgcgaca ccacctgcgt
     3361 cgccagcagc gagtg
//
LOCUS       DASPJI010000367        18798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_137367, whole genome shotgun sequence.
ACCESSION   DASPJI010000367 DASPJI010000000
VERSION     DASPJI010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18798)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18798
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137367"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..854
                     /locus_tag="VEL74_09805"
     CDS             315..854
                     /locus_tag="VEL74_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62863.1"
                     /translation="MPVYDQPSTQSVHPNVFKTQFLARLNERDEPPTAGDADVAGPWR
                     IEAIPGEGFGLFRIGESLARGGSPLAVVPSRWLALVLAAVLPGTGRDPLIRIEKDADE
                     DGAYPLVLPGLDGREVVGRLRVFDEDLVSAVNVAIHLLQAPESLAWLLEAAGGVALER
                     CGAILDERTMETTETTAAE"
     gene            2298..3080
                     /locus_tag="VEL74_09810"
     CDS             2298..3080
                     /locus_tag="VEL74_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HYG62864.1"
                     /translation="MPDETDASDVIGLIPAAGRAERLGPLPCSKELLPVGFRETPGGP
                     APKVASHYLLERFRAAGIQRVFMVVHEAKTDVPRYLKTGELADVSLAYVVIPGSGSVP
                     ETLDRAWLFLQDARVALGFPDILFEPVDAYARLIERQEATGADLVLGLFPTERHRTTD
                     MVELAPDGRVRRIEVRPETTALRYNWLIAVWGPAFTRHLHETVRSAPSSDRELQIGAV
                     VQAAVEAGLHVQGVEFPEGSFRDIGTPGDLAAAMREGAGLGL"
     gene            complement(3087..3437)
                     /locus_tag="VEL74_09815"
     CDS             complement(3087..3437)
                     /locus_tag="VEL74_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HYG62865.1"
                     /translation="MSQPDFHEHLFDIQQACSLIQEFTMGLTFDEYRDDARTYSAVER
                     QFEILGAALPRMLQAFPELASALPDAPHIVELREVLIQATAPSHGAVWSALESHLPQL
                     AREVEMLLDLDASS"
     gene            3528..4583
                     /locus_tag="VEL74_09820"
     CDS             3528..4583
                     /locus_tag="VEL74_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYG62866.1"
                     /translation="MKKQPKIRLAVVGCGAVAAIHHLPAIAASDLVEAAVLVDADEKR
                     ARTLAERWGVPTVLTDYREAVGRVDAAVVAVPNHLHAPVAGDLLRRGVHVLVEKPMAL
                     TGAECGAMIEAAEAGRAVLAVGLEFRFFDSTRFVRDLLASEVIGPLRRFELRQGVSSR
                     WPFATDFLLRKEMSGGGVLMDFGVHVLDLLLAWLGDWESVRYRDDAMGGVESDCEIEL
                     TMRSGLTGFVEISRTRNLANTCRFEGEKGTLEVGIWDPDPEIALRLDGQEMLLSGRAR
                     ARGTTGRAKGGGLEFGSSFVRQVDDFAAAIREGREPRVSGREGRRSMDLIDACYAVRE
                     PLELPWMNPPQLLEAGA"
     gene            4580..5767
                     /locus_tag="VEL74_09825"
     CDS             4580..5767
                     /locus_tag="VEL74_09825"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYG62867.1"
                     /translation="MSLLQGKRVLVTGAGGFIGGRLVERLVLEQGAEVRALVRSVAGA
                     VRVARFPVTVLRGDVTDAGALAEAARDCDAIFHCAYGTSGSQKHRSWVNREGTRRILD
                     AAAAAKVRRVVYLSTLMVYGKTADGDLDETAPRRRFGNEYSDSKLDAEETALGYARAG
                     KVPVTVLQPTAVYGPYGGVWTETVLRTLESGRVILIDGGRGLCNHVYVDDLVSAMLLA
                     AVRDEAVGEAFLVSAEEPVTWTEMYGRFERMLEGESRTVSMTQEEARAHWKQSQREHP
                     RALGELLRAVKGDRAFRDRLMRTRELLTLRELASNTLPEAWQQRIKGRLGMGSAPAPV
                     ARPSGDLPIHPLSPLMIGFFSTRSRVRIDKAKRLLGFRPAFDFEAGMDLTERWARWAN
                     LVP"
     gene            5746..7182
                     /locus_tag="VEL74_09830"
     CDS             5746..7182
                     /locus_tag="VEL74_09830"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HYG62868.1"
                     /translation="MGEPGALTPSPEPGAPSTEQRVLRSTLAGYSIQLARLLVTFGAK
                     LLLARLILPEGHGLYELALRIVTIASAFRDLGLPYHLVRDARRPYGTVLAVTTFLGGL
                     ITVGLIVAAPATSVLTPELPAVLRVMAFWVLLDGLAVVPRAFFERELSIGRLIGPEVW
                     RGVIIAAVSLSLAWLGWGVWSFVIADLAGALLLAVWSWSRTWGRIPLKPDLHLVPDLI
                     RRSSWLFFIWVVIQLVTYVDIFIIEVFGNTEQVGLYSNSYRIAFLVATLVYPRALFPT
                     LVEYLGDPPRFFDSFRLSTLQLLGFQVLGSYFLLFNAATSIRILLGEGWEGAVPMLQV
                     IAFIPFFDQFTILGGEMLKARHEDRAWLWAVALNLVSLVVLGVIFTQRWGAVGMGAAN
                     YLLVGNLLMGWWIWRVFRSRFWTILADLAQLYLIPLPFFAAAAWLFPEGSWARFAASI
                     GAAALATSALAARYHRVFRAFFGRRGQA"
     gene            complement(7183..8568)
                     /locus_tag="VEL74_09835"
     CDS             complement(7183..8568)
                     /locus_tag="VEL74_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62869.1"
                     /translation="MKRKVLCSLAVSALVLAAPAFAGTVYVPVVDQDGTNGSSHTTEV
                     WVSNSGTADRAFSTLFLAADSDGTKRTAKPQQANVQAGKTSVLNGLSGSGKVGLLEVT
                     STSRFLIEARLTSAHINANAISYATLPVISSDNALAGGQWAYLVGLERDAAKGDHTGL
                     AVVNLGKTTSQCQIKVVRANGTQVAGTSIVSVPPLSLRYYTDALALLNEPGAKDARAE
                     VSCNQQFFAFASVFKASNSQYLFLVPSSGGASTLTAPGEQPDTNEPGSSGGLVFTKSG
                     LIHTVTQTNQKGIVDIPVPRAMGLRRMIVDVDFIPGPWNRIRTPANHNIIWIHRNVGA
                     NEFRSNSVVNVNAFSGKNTIKMNQNVDMPTRTQTVGEQGADLIQGQTYHLRYIYDAQN
                     ANVTAVVTHNGNPIVNMRMDGTANNRSLFVEPNKLRAEFGHYNFQAHEGPEVASFGWQ
                     YLNLKVELVPY"
     gene            8823..10073
                     /locus_tag="VEL74_09840"
     CDS             8823..10073
                     /locus_tag="VEL74_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62870.1"
                     /translation="MKKAFVHATLCALAMAVPAAASERVFVPALGTLSEDGSALATKV
                     WVTDAEGKGMLRPAAAREEAGLIALEADETQDVSAWMVGGAKVAAEVPVFTDREAYSA
                     GVDVLLADLPSPKSMTRLFVGAANLSDHVASCVATLYRPNGNLLAEIPFEVAPESFTH
                     EDAFTRTGKARVGEVRVTCDQTFYPVGAATENDKPQVVFAKAFGPNGPCNYNVMLTRQ
                     PATGFYTAASQAGTFHDATRNNPKGIICVKVPQELRLAKAIFEWDVKVGPWSSKNKAG
                     MHNVAYYFLERYRSGVVGNINAAGGKKRLVKFMQNVGMPRKQNTNAKAGYELKEGLTY
                     HFTYVFDAANKTATLQVFLNGTEVTRMFKDVKPGGQQLVLKPYAKEGLAMVAEFGNFN
                     NQHDPEVPSWGWDFANWKMTAIPK"
     gene            10177..12717
                     /locus_tag="VEL74_09845"
     CDS             10177..12717
                     /locus_tag="VEL74_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HYG62871.1"
                     /translation="MPGRAGFVAGVLLGCTLWAGLWAASGRAPSATASPPESPVRQAA
                     PARPATVPWDPPGEGFIVWESNRSGRWRLWTRRLDGTGLRQLTPEAPEAPEDSDRPHC
                     CAHVSPDGRWISYLSLEPGPERYPEGRPETGVLRLIRPDGSGNRVLAPAARAYGEHRA
                     AVWHGPGALVYIDGEGHTVRLDVATGKSERLTTAPHPMSGWLINPGLTHAVAGVPTFS
                     PYDPERRKVTELQALGGCQAYFTQDGRWGYWIAGAGGPIHKMDLRSRSVSVLFGKNDE
                     RMPGGQGYLYFPMLSADRRLLAFGASAGEHDHFTADYDIYVTEVDPERLEVLGPVVQV
                     APHPATDRFPDVYLAPVPLGVHTGEAPFTVRLTAPVQGDWNLGDGSQAQGAAVEHTWT
                     RPGSYRVVVRSGQQALEGRVRVTAPRPPEVEGVTVRERRLVVVRFDEPVETGGLRATL
                     ASGREVRGARLEGGGRELVIELAAPLTSPDTLRLDGITDRAGRPNPMPAREIALAPDV
                     WPVDRDGLLFLWENAGAPNRVFDPLQNVERSFPVESRGEARIDAHQRMDLGAGAFVAA
                     NEASEAVVEGARRANQLTLEVTLEPRRRAQNAPIVSLAGAGAQKTNLVLAQAGEELVL
                     RFGISQHGFVEKEYRLGRVAPGRSTHLVVSYRPGLLAAYRDGERVVETDLQGDFFRWR
                     PRPLVFGADTRSENPWSGFLEGIAFYGRAFGPEESRESARRSLERAGKRPEVPAVRIR
                     GRLLARSRIPTLQDISPYREALAVYEYRVEEVIAGAAPPVSPGNVVRVAHWVILDGRT
                     LDTARRREGETFEILLTPFARNPQLESIFLSDTLPADRTRVLQFDAGR"
     gene            12721..14175
                     /locus_tag="VEL74_09850"
     CDS             12721..14175
                     /locus_tag="VEL74_09850"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HYG62872.1"
                     /translation="MVFSSHLFVFYFLPVVLLLYYVLPSRTRHWTLTLSSYVFYGWAN
                     PYFVLLMLVSTLIDYFAGLAIAHGGRMSLRQPAELLTPGGPRNRAQRIALIVSICGNL
                     GLLGFFKYFNFAADNYNALVGALGISQLAWEETLRVTLPLGISFYTFQSMSYTIDVYR
                     GEARALRSLLDFACYVSMFPQLVAGPIVRFSEVADQLQSRTHTLEKLARGCSMFCLGM
                     AKKVLLANPCGQVADAAFEAGSLAAPDAWFGVAAYAFQIYFDFSGYSDMAIGLGLMLG
                     FMFPKNFDSPYRAESVTDFWRRWHISLSTWLRDYLYIPLGGNRRGVRRTYVNLLLVML
                     LGGLWHGASWNFLLWGGFHGAMLAGERAAGGSLYRWLPRPLRMAATFVLVLLSWVLFR
                     ARDLPHAMGYYQALFSAAPLQEGAALLGGILYQPYFLLCVALAGLIAWLAPQAWDWTR
                     RLTWVRASACAGLFLAALLAMETQGYNPFIYFIF"
     gene            14175..15485
                     /locus_tag="VEL74_09855"
     CDS             14175..15485
                     /locus_tag="VEL74_09855"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62873.1"
                     /translation="MQTDPPPAPVPYLHREVQAREELGHTAVSPTVAGLLAGLFLGLV
                     VGVPALQLTGGWAGGNPFRQLAESLPGAMEASRQEGLWSANRTLLASMNRFEDRLEEE
                     SWVVERLLPPVQGVLTGVLGVGNQQVYTGRDGWLFYRPAVDSLTGPGFLGSGVLAARR
                     AGGDAWEAPVEPDPLPALLGLARDLERRGIRLLVLPVPVKAAIHPERLTRRPARPPLR
                     NPSFGELVRSLEAAGIEVLDLGPLLTEAARREGRPQYLATDTHWAPGGMDAAARAVAG
                     WVERRLPPGRTRAYRSEERTVRGIGDIARMLRLPAGQRLYPPQDVTVTRVSEPDGSPW
                     RSDLEAEILLLGDSFTNVFSQPDLGWGSGAGFAEQLSLRLQRPIDRIAVNAGGAHASR
                     QSLTRALAAGEDRLAGKRLVIYEFSARELAVGDWRRLGLAPGGD"
     gene            15488..16183
                     /locus_tag="VEL74_09860"
     CDS             15488..16183
                     /locus_tag="VEL74_09860"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYG62874.1"
                     /translation="MAGPRSLGRRAFKLFYITYYKLLYGRRFPLADRAERWVRDWEQE
                     TGRGDTPASKDTWEEQYQKGGWEFMRRLDELPRYSVLAGYLRHLKPGGSVLDVGSGEG
                     ILRDEMRVHGYARWVGVDISETAVARAREVPDDRATFLAANAEEYRPAGRDDRFDAVV
                     FNECVYYFEDPLGTVERYRGFLEPGGVILVSMFRSRRADVIRDRLKAALPLREEVELR
                     HRKGTWVVSVFGG"
     gene            16296..16994
                     /locus_tag="VEL74_09865"
     CDS             16296..16994
                     /locus_tag="VEL74_09865"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HYG62875.1"
                     /translation="MLYRVLRIRTATETGSAFSIEIDGRQYLITARHVLRGFGAEGDV
                     DLWIEGRWTRAHARAIYPRKEVVDMAALDFGRPVTVTFPLEASSGGLTLGQQAYFLGY
                     PYGLSSDASSVPPGFGEIPFLKSGIVSAMDYRDPEANVLYLDGHNNPGFSGGPIVFWH
                     SGSRRFRVAGVVRGYRNEALPVLKRKDLNDPDAKAHNDLYTKANSGIVVGYDIRHIIE
                     AIRGANPRILPPGG"
     gene            17030..17590
                     /locus_tag="VEL74_09870"
     CDS             17030..17590
                     /locus_tag="VEL74_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62876.1"
                     /translation="MEKRPSLGNLWKVVVTVLTVATTLMALYVQLFERRSRQEEDRLA
                     AVRLEAALAESRARLKTEILAELRAELARAAAEQPDGQPRPDTVLRRPESDGNGPLQQ
                     APLGLPSGRSSERLDSLARQMEDSDRALRQDLEELRAASRREAEISSRATSLILLALV
                     LLAAHLLVSLWRPRQRGQGTAPRPEG"
     gene            complement(17634..>18798)
                     /locus_tag="VEL74_09875"
     CDS             complement(17634..>18798)
                     /locus_tag="VEL74_09875"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYG62877.1"
                     /translation="DGLPAKANTLAATDLDGDGAEEILLGEPGKLWALGSPDSPTAPR
                     PLLEASSFDLRRWSSGTPGLFQAPEAGRLRTWRLAGGRLVPGPEHSLPLRVARERQAL
                     RFSSLPVTAIPGPGGAPPFQAVGPESNGKLRLRTVLLGPDGQQTEAWSRLPGREDVDS
                     FRYVTLDGRPALIVMTSDAEKVGIFAKQRFRLFPLNADRSRAGQPPSLAFETESNRWF
                     QVDPVLRDLDGDGKQDLVVIQPEGMGGGDLVIDTFFGQGDGRFERPRRQKLSNLDSRS
                     WIYSGDVTGDGVADLVAVAKSGLRVFAGTREPRRELLDRRSRPAIGVSGEREMVTVAL
                     GVGGEGANMTSSRPTNLGAPRLEDLDGDGRSEILMSSPSSGGRGRVTVVRLGG"
BASE COUNT         2696 a         6540 c         6496 g         3066 t
ORIGIN      
        1 cacggaaacg atagcacgga ggcggtgcgg cgacccatcc ggccatcgtc gcggatgagt
       61 cgaaaactcc gccccaccct cttgactccc cattttcacc tcacatagga tcgcatcact
      121 cgaatgtcgc cgtaggacga tcgaggcccg gcagcccctc ggaaggtgtg agcgggccgg
      181 tagccgggaa tcgcggcttc cggtgatgtt cctggatgtg gggtcggccg gcaatggatg
      241 ccggtttagc ggcccgggag tgagctggtg actcaccccg agcctagacc acacagtcct
      301 cagaaggagg acttatgccg gtctatgacc agcctagcac gcagtctgtc catccgaacg
      361 tcttcaaaac ccagttcctc gcccgtctga atgagcggga cgagccgccg acggccggtg
      421 acgccgatgt cgccgggcca tggcggatcg aagcgattcc gggagagggc ttcggcctgt
      481 tccggatcgg cgagagcctg gcccgggggg gctcccccct ggccgtggtg ccgtcccgct
      541 ggctcgcact cgtcctcgcc gccgtgctcc ccggcacggg gcgggacccg ttgatccgca
      601 tcgagaagga cgccgatgag gacggcgcct atcccctcgt gcttccgggc ctggacggcc
      661 gggaggtggt aggacggctc cgggtcttcg acgaggatct cgtcagcgcc gtgaacgtcg
      721 cgatccacct gctccaggcg ccggagtccc tcgcctggct gctggaggcg gcgggcggcg
      781 tcgccctgga gcggtgcggg gcgatcctcg atgagcggac gatggagacg acggagacaa
      841 cggcggcgga gtagggccgt tcccggctac cggcaggccg aggcctgccg gtagctcttt
      901 tccggatcag gcagagccct tccgcgcctt tagacggttg gattgtctcc acgtacgagg
      961 actgctgcgg tcagtggcac cgctgcgagg gtgcggcgcc ggcggtgacg gatctacttc
     1021 aacctagaag cttggactgt gggcgccccg cagtttagct cgcccaacag ttggtgacca
     1081 tggtgttcgc cggtttaaca ggggttccac caccacaaaa gcaagtaagt gtgtcggtgc
     1141 cgcctgtgtt ataggttgta cacattatcg tgtcggcgcc cgcaatgtct ttcatggatc
     1201 tgccgtcgag acccctcagg gtctccctcg tcaaccccag cttctttacc gtcttcttca
     1261 tagtcggctc cttagtaggt acccgtcgaa ccagtgcccg cacccgccct acctccttgg
     1321 gggtcggaaa ccgccctcga caaccggcac aatagcatat ttctaacagc gaatagtccg
     1381 ctcctccggg tccattcaac cgcccgccgg ctgtgccgcc cgcgacatct ggaggcgagc
     1441 cgcgcagcgg ccgcaagagc cgccacgctc cgcctagcgg tccaacattt gcatttgatt
     1501 ctttgctgtc cgaaggcgct ggagccttcg gccgagaagg aggcacgacc atgaaactca
     1561 agagaaggat tgtacagacc gggtacatgg gtaacagatt ggaccgggta catgggtaac
     1621 actcagaagc ccttctacta catagattta cgctccccgg gcaggttgaa gaagtggcct
     1681 gtggaagctg cggaaagctg atatctttca aagacttagg tcacctttcc gcagctccca
     1741 caggcccgac ggcgacgggc tcaaggaccg caggagaccg gcagaacagg ctttctgagt
     1801 gtcacccatg tacccggtct gaagtgttac ctaggtcccc ggctgtacac tcccgtcacc
     1861 tcgatgccat cccggagctt cggactcgat cccttggagc tggacgcaat ccggagcgct
     1921 cggaaagctt cccgggactc gggaatccat ccgcttgaat gggaccgcga tccggagcgc
     1981 tcggactccg tccggatgag ctggacgcga tccatggcgt tcgggagctt cccgggccgt
     2041 ccggatggta tccgagcgac tcggacgcca tcccgagcgt tcgggaaatg tcccggggct
     2101 tccggattcc atccgcggcg ttcggacgag atcctggacg tccgggcgag accgcagcgt
     2161 ccaaaacccc cctcctggcc tcccccgctc cctaccgtcc acccttccag gggggaggca
     2221 ccaccgcccc cttgcgccac aagtcaacgc gtagggggac gactgcctct tacaatcgcc
     2281 gcgagaggag gacgaccgtg cccgatgaaa ccgacgcgag cgacgtgatc ggcctgatcc
     2341 ccgcggcggg gcgggccgag cggctggggc cgctgccgtg cagcaaggag ctgcttcccg
     2401 tgggattccg cgagaccccc ggcgggccgg cgccgaaggt ggcgagccat tacctgctgg
     2461 agcgcttccg ggcggccggg atccagcgcg tcttcatggt ggtccacgag gccaagacgg
     2521 acgttccccg ttatctgaag acgggcgagt tggcggacgt ctccctcgcc tacgtggtga
     2581 tccccggctc agggagcgtc cccgagaccc tggaccgggc ctggctgttc ctccaggacg
     2641 cccgcgtcgc cctcggtttc ccggacatcc ttttcgagcc cgttgatgcc tacgcgcggc
     2701 tgatcgagcg ccaggaggcc acgggcgccg acctcgtcct gggactgttc ccgacggagc
     2761 ggcaccgcac caccgacatg gtcgagctgg cgccggacgg ccgggtgcgc cggatcgagg
     2821 tccgcccgga gaccacggcc ctgcgctaca actggctgat cgccgtctgg gggcccgcct
     2881 ttacccggca tctccacgag accgtgcgtt cagccccgtc gtccgaccgg gagctccaga
     2941 tcggcgccgt ggtgcaggcc gccgtcgagg cggggctgca cgtccagggg gtagaattcc
     3001 ccgaagggag cttccgggac atcggcaccc cgggggacct ggccgcggcg atgcgggagg
     3061 gggctgggct cgggctctga ggatcctcag gagctggcgt cgaggtcgag cagcatctcg
     3121 acctcgcggg cgagctgggg gagatggctc tcgagggcgc tccagacggc gccatgggag
     3181 ggggcggtcg cctggatcag gacctcgcgc agctccacga tgtggggggc gtcgggcagg
     3241 gccgaggcca gctcggggaa ggcctgcagc atcctcggca gagccgcgcc gaggatctcg
     3301 aactgcctct cgacggccga gtaggtccgg gcgtcgtccc ggtactcgtc gaaggtcagc
     3361 cccatggtga actcctggat caggctgcag gcttgctgga tgtcgaacag gtgctcgtgg
     3421 aaatcgggtt ggctcataga ggtgggagga tatccgatgc cgggccgttc tccccaggtc
     3481 tgataccatt cgccgccgct ccggcaccgc cggggactga cctccacatg aagaagcagc
     3541 ccaagatccg cctcgccgtg gtcggctgcg gcgccgtcgc cgccatccac cacctgccgg
     3601 ccatcgccgc ctcggacctc gtcgaggccg ccgtgctcgt ggacgcggac gagaagcgcg
     3661 cccggacgct cgccgagcgc tggggcgtcc ccacggtgct caccgactat cgcgaggccg
     3721 tggggcgggt ggatgccgcc gtggtcgccg tgcccaacca cctgcacgcg ccggtggccg
     3781 gcgacctgct gcggcgcggc gtccacgtgc tggtggagaa gccgatggcc ctcaccggcg
     3841 ccgagtgcgg cgcgatgatc gaagccgccg aggccggccg ggccgtcctc gcggtggggc
     3901 tcgagttccg gttcttcgac tccacccggt tcgtccgcga cctgctggcc agtgaggtca
     3961 tcggcccgct ccggcgcttc gagctgcgcc agggggtgag ctcccgctgg ccgttcgcca
     4021 ccgacttcct gctgcgcaag gagatgtcgg gcggcggcgt gctgatggac ttcggcgtcc
     4081 acgtgctcga cctcctgctc gcctggctcg gcgactggga gagcgtgcgc taccgcgacg
     4141 acgccatggg cggcgtcgag tcggactgcg agatcgagct gacgatgcgc tcgggcctga
     4201 ccggattcgt cgagatcagc cgcacccgca acctcgccaa cacctgccgc ttcgaggggg
     4261 agaaggggac gctcgaggtg gggatctggg acccggaccc cgagatcgcc ctgcggctcg
     4321 acggccagga gatgctcctc tccggccgcg cccgcgcccg ggggacaacc ggcagggcga
     4381 agggaggagg cctcgagttc ggcagctcct tcgtccgcca ggtcgacgac ttcgccgccg
     4441 cgatccgcga ggggagggag ccgcgggtct ccggccgcga ggggcggcgc tccatggacc
     4501 tcatcgacgc ttgctacgct gtccgcgagc cgctggagct cccctggatg aatccgccgc
     4561 agctcctgga ggccggagcg tgagcctgct gcaggggaag cgggttctgg tcaccggcgc
     4621 cggcggcttc atcggcggcc ggctggtgga gcggctcgtc ctggagcagg gcgccgaggt
     4681 gcgcgccctg gtgcggagcg tcgccggcgc cgtccgcgtc gcccgctttc ccgtcaccgt
     4741 cctgcgcggc gacgtcaccg acgccggtgc cctggccgag gcggcccggg actgcgacgc
     4801 catcttccac tgcgcctacg ggacgtcggg cagccagaag caccgctcgt gggtcaaccg
     4861 cgaggggacg cgccgcatcc tggatgccgc ggccgccgcc aaggtccgcc gggtggtcta
     4921 cctgagcacc ctcatggtct acggcaagac cgccgacggc gacctcgacg agaccgcgcc
     4981 aaggcgccgc ttcggcaacg agtactcgga ctccaagctc gacgccgagg agaccgccct
     5041 ggggtacgcc cgcgccggca aggtgccggt caccgtcctc cagcccaccg ctgtctacgg
     5101 cccctacggc ggcgtctgga ccgagacggt gctgcggacg ctggagagcg gccgggtgat
     5161 cctgatcgac ggcggccggg ggctctgcaa tcacgtctac gtggacgacc tggtgagcgc
     5221 catgctgctc gccgcggtgc gcgacgaggc ggtgggggag gccttcctgg tctccgccga
     5281 ggagccggtg acctggaccg agatgtacgg ccgcttcgag cgcatgctgg aaggggagtc
     5341 ccggacggtc tcgatgaccc aggaggaggc gcgggcgcac tggaagcagt cccagcgcga
     5401 gcacccccgc gccctcggcg agctgctgcg ggcggtcaag ggggaccgcg ccttccgcga
     5461 ccggctgatg cggacgcgcg agctcctcac cctccgcgag ctggcgagca acaccctccc
     5521 cgaggcctgg cagcagcgga tcaagggtcg cctcggcatg gggagcgccc cggcgccggt
     5581 ggcgcgcccg agcggcgatc tgcccatcca cccgctgagc ccgttgatga tcggcttctt
     5641 cagcaccagg tcccgggtgc ggatcgacaa ggcgaagcgc ctcctgggct tccgtcccgc
     5701 cttcgacttc gaggccggca tggacctgac cgagcggtgg gcgcgatggg cgaacctggt
     5761 gccctgacgc cgagccccga gcccggggct ccttccaccg agcagcgggt cctgcgctcg
     5821 accctggccg gctactcgat ccagctcgcg cggctgctgg tcaccttcgg cgccaagctc
     5881 ctcctggcgc ggctcatcct ccccgagggg cacggcctct acgagctggc gctgcgcatc
     5941 gtcaccatcg cctcggcgtt ccgcgacctg gggctgccgt accacctggt gcgcgacgcc
     6001 cgccggccgt acggcaccgt cctcgcggtc accaccttcc tcggcgggct catcaccgtc
     6061 gggctgatcg tggcggcgcc ggcgacgtcg gtgctcaccc ccgagctgcc ggcggtgctg
     6121 cgggtgatgg ccttctgggt gctgctcgac ggcctcgcgg tcgtcccccg cgccttcttc
     6181 gagcgcgagc tgtccatcgg ccggctgatc ggccccgagg tctggcgcgg cgtgatcatc
     6241 gcggccgtct ccctgagcct cgcctggctc ggctggggcg tctggagctt cgtcatcgcc
     6301 gacttggcgg gcgctctcct gctggcggtc tggagctggt cgcggacgtg ggggaggatt
     6361 ccgctcaagc ccgacctcca cctggttccg gacctgatcc gccgcagctc ctggctcttc
     6421 ttcatctggg tcgtgatcca gctcgtcacc tacgtcgaca tcttcatcat cgaggtgttc
     6481 ggcaataccg agcaggtggg gctctactcg aactcctacc gcatcgcctt cctggtggcg
     6541 accctggtct atccgcgggc gctcttcccg acattggtcg agtacctggg ggacccgccg
     6601 cgcttcttcg actccttccg gctcagcacc ctccagctcc tcggcttcca ggtgctgggg
     6661 agctatttcc tgctgttcaa cgccgccacc tcgatccgca tcctgctggg ggagggttgg
     6721 gaaggggcgg tccccatgct ccaggtgatc gccttcatcc cgttcttcga tcagttcacg
     6781 attctcggcg gcgagatgct caaggcgcgg cacgaggacc gggcctggct ctgggccgtg
     6841 gcgctcaacc tggtctcgct ggtggtgctg ggagtgatct tcacccagcg ctggggggcg
     6901 gtggggatgg gggcggccaa ctatttactg gtggggaacc tgctcatggg ctggtggatc
     6961 tggcgggtct tccggagccg gttctggacg atcctggcgg acctcgccca gctctatctc
     7021 atccccctgc cgttcttcgc cgccgcggcc tggctgttcc ccgaaggctc gtgggcgcgt
     7081 ttcgccgctt ccatcggagc ggcggccctg gccaccagcg cgctggcggc caggtaccac
     7141 cgggtgttcc gcgccttctt cgggcggcgc gggcaggcgt gatcagtaag gcaccagctc
     7201 caccttcaga ttgaggtact gccagccgaa gctcgccacc tccgggcctt catgggcctg
     7261 gaagttgtag tgcccgaact cggcccggag cttgttgggc tcgacaaaga gcgaccggtt
     7321 gttggccgtg ccgtccatcc tcatgttcac gatcgggttg ccgttgtggg tgaccaccgc
     7381 ggtcacgttg gcgttctgcg cgtcgtagat ataccgcagg tgatacgtct gcccctggat
     7441 cagatcggcg ccctgctcgc ccaccgtctg ggtacgcgtc ggcatgtcca cgttctggtt
     7501 catcttgatc gtgttcttgc cggagaacgc gttcacgttg acgacggagt tgctgcgaaa
     7561 ctcgttcgcg cccacgttgc ggtggatcca gatgatgttg tgattggccg gggtgcggat
     7621 gcggttccag gggccgggga tgaagtccac gtccacgatc atgcggcgca gccccatggc
     7681 gcgcggcacc gggatgtcca cgatcccctt ctgattagtc tgggtgaccg tgtggatgag
     7741 gccggacttc gtgaagacca ggccgccgga gctgccgggc tcgttggtgt ccggctgctc
     7801 gcccggcgcc gtcagggtcg aggcgccgcc gctcgacggc accaggaaga gatattgcga
     7861 gttgctcgcc ttgaacaccg aggcgaaggc gaagaactgc tgattgcagg agacctcggc
     7921 gcgcgcgtcc ttggcgcccg gctcgttgag gagcgccagg gcgtccgtgt aataacggag
     7981 cgagagcggc ggcacgctga cgatgctcgt gccggcaacc tgtgtgccgt tggcgcggac
     8041 gaccttgatc tggcactggg aggtggtctt gccgaggttg accaccgcca gtccggtgtg
     8101 gtcccccttc gccgcgtcac gctccaggcc gaccaggtag gcccactggc cgccggccag
     8161 cgcgttgtcc gacgagatca ccggcagggt ggcgtaggag atcgcgttgg cgttgatgtg
     8221 ggcgctggtc agccgcgcct cgatcaggaa cctggaggtg ctggtgacct ctagcagccc
     8281 caccttgccg gagccgctga gcccattcag caccgaggtc ttgccggcct ggacgttggc
     8341 ctgctgcggc ttggcggtgc gcttggtgcc gtccgagtcg gcggcgagga acagggtgga
     8401 gaaggcgcgg tcggcggtgc ccgagttgga gacccatacc tccgtcgtgt ggctgctgcc
     8461 gttcgtcccg tcctggtcga ccacggggac gtagaccgtg cccgcgaagg cgggggcggc
     8521 gagcaccagg gcggagacgg cgagactgca cagaaccttc ctcttcatcc atcgacctcc
     8581 tgttcagacg acggctgcgg cacagcgggt acagagattg tagaggagat ggtacctatg
     8641 caggaccttc gatgcaagcg cggcggcggg gccggcgagg agaaatgtgc acgaagagaa
     8701 tctcttttgc acaacagggg ttgcatccgc agctttcaag ccctactata accccacgtc
     8761 gcaggccacg tagcttgtta ggcaacaaga agggtagcaa accgtctagg agggttccac
     8821 cgatgaagaa ggcattcgtc cacgcgacac tgtgcgcact cgccatggcc gtaccggccg
     8881 ccgcctctga aagggttttc gttccggctc tgggcaccct gtccgaagac ggcagcgccc
     8941 tggccaccaa ggtgtgggtc accgacgcgg aaggcaaagg catgctgcgg cccgccgcgg
     9001 ctcgggagga ggcggggctg atcgccctcg aagccgacga gacgcaggac gtgagcgcct
     9061 ggatggtggg aggcgccaag gtcgccgccg aggttccggt cttcaccgac cgcgaggcct
     9121 actccgccgg ggtcgacgtg ctgctcgccg acctgccgag ccccaagtcc atgacgcgcc
     9181 tcttcgtcgg cgccgccaac ctgtccgacc atgtcgcctc ctgcgtcgcc accctctacc
     9241 gtcccaatgg caacctcctc gccgagatcc ccttcgaggt ggctcccgag tcgttcaccc
     9301 atgaggatgc cttcacccgc accggcaagg cccgcgtcgg cgaggtccgc gtcacctgcg
     9361 accagacctt ctacccggtc ggcgccgcca ccgagaacga caagccgcag gtggtcttcg
     9421 ccaaggcctt cggcccgaac ggcccctgca actacaacgt gatgctcacc cgccagccgg
     9481 ccaccggctt ctacaccgcg gcgagccagg ccggcacctt ccacgacgcg acccgcaaca
     9541 atcccaaggg gatcatctgc gtcaaggtgc cgcaggagct gcgcctcgcc aaggccatct
     9601 tcgagtggga cgtcaaggtc ggcccctgga gctcgaagaa caaggccggc atgcacaacg
     9661 tcgcctacta cttcctggag cgctaccgta gcggcgtggt gggcaacatc aacgccgccg
     9721 gcggcaagaa gaggctggtc aagttcatgc agaacgtcgg catgccgcgc aagcagaaca
     9781 ccaacgccaa ggccggctac gagctgaaag agggcctgac ctaccacttc acctacgtct
     9841 tcgacgccgc caacaagacc gcgaccctcc aggtgttcct caacggcacc gaggtcaccc
     9901 ggatgttcaa ggacgtcaag cccggtggac agcagctcgt cctcaagccg tacgccaagg
     9961 aaggcctcgc gatggtggcc gagttcggca acttcaacaa ccagcacgat cccgaggtgc
    10021 cctcctgggg ctgggacttc gccaactgga agatgaccgc catcccgaag taagccgcgc
    10081 cgcgaggctt ggctccgaga cgaaggccgc ggctgcccgc cgcggccttt tctctttttc
    10141 atccgctacc atgtgcgccg catgaggaga ggttctttgc ctggacgggc tggtttcgtg
    10201 gcgggggtgc tgctgggctg caccctgtgg gccggcctgt gggccgcttc gggaagggct
    10261 ccgtccgcca ccgcctcccc gccggagagc ccggtccgcc aggcggcgcc cgccaggccg
    10321 gcgacggtgc cctgggaccc gccgggcgag ggcttcatcg tctgggagtc gaaccgctcc
    10381 ggccgctggc gcctctggac ccggcgcctc gacggcacgg ggctgcggca gctcaccccg
    10441 gaggccccgg aggcccctga ggatagcgac cgcccgcact gctgcgccca cgtctcgccc
    10501 gacggccgct ggatctccta tctgagcctg gagccggggc ccgagcgcta tcccgagggg
    10561 cggcccgaga ccggcgtcct gcgcctcatc cgccctgacg gctccggcaa tcgggtgctg
    10621 gcgcccgccg cccgcgccta tggggagcac cgcgccgccg tctggcacgg tcccggagcc
    10681 ctggtctata tcgacggcga gggtcacacc gtccgcctgg acgtggcgac gggaaagagc
    10741 gagcgcctca ccacggcgcc gcaccccatg tccggctggc tgatcaatcc cggcctgacg
    10801 cacgcggtgg cgggcgtgcc caccttttcc ccctatgacc cggagcggcg caaggtgaca
    10861 gagctccagg ccctcggcgg ctgccaggcc tacttcaccc aggacggccg ctggggctac
    10921 tggatcgccg gcgccggcgg gccgatccac aagatggacc tgcggagccg ctcggtctcg
    10981 gtgctcttcg gcaagaacga cgagcggatg cccggcggcc agggctacct ctacttcccc
    11041 atgctgtcgg cggaccggcg cctcctggcc ttcggcgcct cggcgggcga gcacgaccac
    11101 ttcacggccg actacgacat ctatgtgacc gaggtggacc cggagcgtct ggaggtgctc
    11161 gggcccgtgg tccaggtcgc cccgcacccc gccaccgacc gcttcccgga cgtctacctg
    11221 gcgcccgtcc ccctgggcgt gcacaccggc gaggccccct tcacggtccg cctcacagcc
    11281 cctgttcagg gggattggaa cctgggcgac ggctcgcagg cacagggcgc tgccgtggag
    11341 cacacctgga cgcgccccgg ctcctaccgg gtggtggtcc gctcggggca gcaggctctg
    11401 gaggggcggg tgagggtgac ggcgccgcgc cccccggaag tggagggggt gaccgtgcgc
    11461 gagcgccggc tcgtggtggt gcgcttcgac gagccggtgg agaccggagg cctgcgcgcc
    11521 accctggcct cgggacgaga ggtccggggg gcccgtctgg aagggggcgg ccgggagctg
    11581 gtgatcgagc tggcggcgcc gctgacctct ccggacacgc tgcgcctcga cggcatcacc
    11641 gaccgggcgg ggcggcccaa ccccatgccc gcgcgggaga tcgccctggc ccccgatgtc
    11701 tggccggtgg accgcgacgg cctcctcttc ctttgggaga acgccggcgc tcccaaccgc
    11761 gtcttcgatc ccctgcagaa cgtggagcgg agcttcccgg tggagagccg gggggaggcc
    11821 cggatcgacg cccaccagcg catggacctg ggggcgggcg ccttcgtcgc ggccaacgaa
    11881 gcctccgaag ccgtggtgga gggggcgcgg cgcgccaatc agctcactct ggaggtcacc
    11941 ctcgagccgc ggcggcgcgc ccagaacgcg ccgatcgtct cgctcgccgg agccggcgcg
    12001 cagaagacca acctggtgct ggcccaggcg ggggaggagc tggtgctccg cttcggcatc
    12061 tcccagcacg gcttcgtaga gaaggagtac cggctgggcc gggtcgcccc gggccgctcc
    12121 acccacctgg tggtgagcta ccggcccggc ctcctcgccg cctaccggga cggcgagcgg
    12181 gtggtggaga ccgatctcca gggggacttc ttccggtggc gcccccggcc gctggtcttc
    12241 ggtgccgata cccggagtga aaacccgtgg agcggtttcc tggaggggat cgccttctac
    12301 gggcgggctt tcgggcccga ggagtcgcgg gagagcgctc gccgctcgct ggagagagcc
    12361 gggaagcggc cggaggtccc cgccgtccgt atccgagggc ggctcctggc ccgctcgcgc
    12421 atcccgaccc tgcaggacat ctccccctac cgggaggcgc tggcggtcta cgagtaccgg
    12481 gtggaggagg tgatcgcggg agctgcgccg ccggtctccc cgggcaatgt cgtgcgggtg
    12541 gcccactggg tgatcctgga cggccggacg ctggacaccg cccgccgccg cgaaggggaa
    12601 acgttcgaga tcctcctcac ccccttcgcc cgcaacccgc agctcgagag catcttcctc
    12661 tcggacaccc tgccggcgga ccggacgcgg gtcctccagt tcgacgcggg gcgctgatcc
    12721 atggtcttca gctcccacct gttcgtcttc tacttcctgc cggtggtgct gctgctctac
    12781 tacgtcctgc cgtcccgcac ccggcactgg acgctgaccc tgtccagcta cgtcttctac
    12841 gggtgggcga atccctactt cgtcctcctg atgctggtct ccaccctgat cgattacttc
    12901 gccggcctgg cgatcgccca tggcggacgg atgagcctgc gccagccggc ggagctcctc
    12961 acgccgggag gcccccgcaa ccgggcgcag cggatcgcgc tgatcgtttc gatctgcggc
    13021 aacctcggac tgctcggatt cttcaagtat ttcaacttcg cggccgacaa ctacaacgcc
    13081 ctggtggggg cgttggggat ctcccaactc gcatgggagg agacgctgcg ggtcaccctg
    13141 ccgctgggga tcagcttcta tacctttcaa tccatgagct acaccattga cgtctaccgg
    13201 ggggaggcgc gggcgctccg cagcctgctc gacttcgcct gctacgtgtc gatgttcccg
    13261 cagctcgtgg ccgggccgat cgtccgcttt tccgaggtgg ccgatcagct ccagagccgt
    13321 acccacacac tggagaagct cgcccgcggc tgctcgatgt tctgtctggg catggccaag
    13381 aaggtgctgc tggcgaaccc ctgcggccag gtggcggacg cggccttcga agccggctcg
    13441 ctcgccgcgc cggacgcctg gttcggggtg gcggcctacg ccttccagat ctatttcgat
    13501 ttcagcggct actcggacat ggcgatcggc ttggggctga tgctgggctt catgttcccg
    13561 aagaacttcg attcacccta ccgggccgaa tcggtcaccg atttctggcg gcgctggcac
    13621 atctcgctct ccacctggct gcgggactac ctttacatcc cgctgggagg gaaccgccgc
    13681 ggtgtgcggc gcacgtacgt caacctcctg ctggtgatgc tcctgggagg gctgtggcac
    13741 ggcgcctcgt ggaacttcct tctctggggc ggcttccacg gcgcgatgct ggcgggggag
    13801 cgggcggctg gtggaagcct ctatcgctgg ctgccgcggc cgttgcggat ggcggccacc
    13861 ttcgttctcg tgctcctctc ctgggtgctg ttccgggccc gcgatctccc ccatgccatg
    13921 ggctactatc aggccctgtt cagcgccgcg ccgctccagg agggggcggc cctgctgggt
    13981 ggcatcctgt accagcccta tttcctgctc tgcgtcgccc tcgccggtct catcgcttgg
    14041 ctggcgccgc aggcctggga ctggacccgc cgcctcacct gggtgcgggc ctccgcctgc
    14101 gccggcctct tcctggcggc gctcctggcg atggagacgc agggctacaa cccgttcatc
    14161 tacttcatat tctgatgcag accgatccgc ccccggcccc cgttccctat ctgcaccgcg
    14221 aggtccaggc ccgcgaggag ctgggccaca cggccgtgag ccccaccgtg gccgggctcc
    14281 tggccggcct cttcctgggg ctggtggtcg gggtgccggc gctccagctc accggcggct
    14341 gggccggggg caacccgttc cgccagctcg cggagagtct gccgggggcg atggaggcgt
    14401 cccggcagga gggcctctgg tctgccaacc ggactcttct cgcctccatg aaccgtttcg
    14461 aggaccgcct ggaagaggag tcctgggtgg tggagcggct gctgccgccg gtgcaggggg
    14521 tgctcaccgg cgtcctgggc gtcggcaacc agcaggtcta taccggacgg gacggctggc
    14581 tcttctaccg gccggccgtg gactctctca ccggaccagg cttcctgggg agcggcgtgc
    14641 tggcggcgcg gcgggcggga ggcgacgcct gggaggcgcc ggtcgagccc gatccgctcc
    14701 ccgcgctcct cggcctggcc cgtgacctgg agcggcgcgg catccgcctc ctcgtgctgc
    14761 ccgtgccggt gaaggcggcg atccatcccg agcggctgac gcgccgtccg gcccggccgc
    14821 cgctccggaa tccctccttc ggcgagctcg tccgctcgct ggaagcggcg ggtatcgagg
    14881 tgctggacct cggacccctc ctgacggagg cggcccggcg cgaggggcgg cctcagtatc
    14941 tcgccacgga cacccactgg gcccccgggg gcatggacgc cgccgcccgc gccgtggccg
    15001 gctgggtcga gcgccgcctg ccgcccggcc gcacccgggc ctaccggagc gaggagcgga
    15061 cggtccgggg gatcggcgac atcgcccgca tgctgcgcct cccggcgggg cagcggctct
    15121 atccgccgca ggacgtgacc gtcacccgtg tatccgagcc cgacggctcc ccctggcgct
    15181 ccgatctgga agcggagatc ctcctcctgg gggacagctt cacgaacgtc ttctcgcagc
    15241 cggacctggg ctgggggagt ggggccggct tcgcggagca gctcagcctc cggttacagc
    15301 ggccgatcga tcgcatcgcc gtcaatgccg gaggcgccca cgccagccgg cagtccctga
    15361 ccagggccct cgccgcggga gaagaccggc tggccggcaa gcggctggtg atctatgaat
    15421 tttccgcccg cgagctggcg gtgggggact ggcgccgtct cggcctcgcc ccgggagggg
    15481 actagacatg gctggaccgc gaagcctcgg ccgccgggcg ttcaagctct tttacatcac
    15541 ctattacaag ctcctctacg gccgccgctt tcccttggcg gaccgtgccg agcgttgggt
    15601 gcgggactgg gagcaggaga ccggccgcgg cgacaccccg gcttccaagg acacctggga
    15661 ggagcagtat cagaaaggtg gctgggagtt catgcgccgg ctggacgagc tgccccgcta
    15721 cagcgtcctc gccggctacc tccggcatct gaaaccgggc ggctcggtgc tggacgtggg
    15781 cagcggcgag ggcatcctga gggacgagat gcgcgtccac ggctacgccc gctgggtcgg
    15841 cgtagacatc tccgagaccg ccgtggcgag ggcccgtgag gtcccggacg accgtgccac
    15901 tttcctggcc gccaacgccg aggagtaccg gccggccggc cgcgatgacc gctttgacgc
    15961 cgtggtcttc aacgaatgtg tctattattt cgaagaccca ctcggcaccg tggagcgcta
    16021 ccgcggcttc ctcgagcccg gcggtgtgat cctggtttcc atgttccgtt cccgccgcgc
    16081 cgacgtgatc cgtgaccgct tgaaagccgc cctcccgctc cgcgaggagg tggagctgcg
    16141 gcaccgcaag ggaacctggg tggtgagcgt gttcggtggg taggggtgct ctcctccggg
    16201 tgctagcatg agcggccatg aggcgagtct tacctgccct gttgtccctg tttctcatcg
    16261 gttccctggc gtgggctcag gcgccgacgt cgaacgtgct ctatcgtgtc ctccggatca
    16321 ggaccgccac cgagacgggg tccgcctttt ccatcgagat cgacggcagg cagtacctca
    16381 tcacggcgcg gcacgtgctg cggggcttcg gagccgaggg ggacgtcgat ctctggatcg
    16441 aggggagatg gacccgggcc cacgcccgcg cgatctatcc gcgcaaggag gtggtggaca
    16501 tggcggctct cgacttcggc cggccggtca ccgtcacctt tcctctcgag gcctcctctg
    16561 gtgggctcac gctgggccag caggcctact tccttggcta tccctacggc ttgagctcgg
    16621 acgcctcctc cgtgccgccg gggttcggcg agatcccgtt cctcaagtcc gggatcgtct
    16681 cggcgatgga ctacagggat cccgaggcca acgtgctcta cctggacggt cacaacaatc
    16741 caggcttctc cggcggcccg atcgtgttct ggcactccgg gagccgcagg ttccgggtgg
    16801 ccggggtcgt gcgcggatac cggaacgagg cgctgccggt gctcaaaagg aaggatctga
    16861 acgacccgga cgccaaggcc cacaacgatc tctatacgaa ggccaactcg gggatcgtcg
    16921 tgggctacga catccggcac atcatcgaag cgatccgcgg cgccaacccc cggattctcc
    16981 cgccgggcgg ttgatccggc ggctggttca ctgacgagag gagccgcgca tggagaaaag
    17041 gccgagcctg gggaacctct ggaaggtggt ggtcacggtc ctcaccgtgg cgaccacgct
    17101 catggcgctc tacgtccagc tcttcgagcg gaggtcgagg caggaggagg accggctcgc
    17161 cgccgtccgg ctggaggcgg cgctcgccga atcccgggcc aggctcaaga ccgagatcct
    17221 tgccgagctg cgggccgagc tggcccgggc cgcggccgag cagccggacg gccagccacg
    17281 ccccgatacg gtcctccgcc ggccggagtc cgacggcaac ggcccgctgc aacaggcgcc
    17341 tctggggtta ccctccggac ggtcgagcga gcgcctcgat tccctggcgc ggcagatgga
    17401 ggactctgac cgggctctcc ggcaggatct cgaagagctg cgggccgcga gccggcgcga
    17461 agcggagatc tcctccaggg cgacgagcct catcctcctc gctctggtcc tgctggccgc
    17521 ccacctcctc gtctccctct ggcggccacg gcagcgcggg cagggaacgg ctccccgccc
    17581 tgagggatga agttgaaggg gctcccgtcc cagcggcgga accggagggg ccctcacccg
    17641 cccagccgca ccaccgtcac ccgtcccctc cctccggagc tcggggagga catcaggatc
    17701 tcggaccggc catcgccgtc caggtcttcg aggcggggag cgcccaggtt ggtggggcgg
    17761 ctggaggtca tgttcgcacc ctcgccgccg accccgaggg cgacggtgac catctccctc
    17821 tccccgctga ccccgatggc cggccgggag cggcggtcca ggagctcccg gcggggctcg
    17881 cgggtgccgg cgaagacgcg gaggccggac ttcgccacgg ccaccaggtc ggccacgccg
    17941 tcgcccgtga cgtccccact gtagatccag gaacgcgagt cgagattgct cagcttctgg
    18001 cgccgggggc gctcgaagcg gccgtctccc tggccgaaaa aggtatcgat caccagatcg
    18061 ccgccgccca tgccctcggg ctggatgacc accaggtcct gcttgccgtc tccatccagg
    18121 tcgcggagga ccgggtccac ctggaaccag cggttgctct cggtttcgaa ggcgagcgac
    18181 ggcggctggc ccgcgcggct gcggtcggcg ttcaacggga acagccggaa acgctgcttg
    18241 gcaaagatac cgaccttctc cgcgtcggag gtcatcacga tcagcgccgg ccgcccgtcg
    18301 agggtgacgt agcggaagct gtccacgtcc tcccggccgg ggagccggga ccacgcttcc
    18361 gtctgctggc cgtcggggcc gaggaggacc gtgcgcagcc ggagcttgcc attgctctcg
    18421 gggccgaccg cctgaaacgg cggcgcgccg ccgggacccg ggatggccgt caccggcagg
    18481 gaggagaacc gcagggcctg gcgctcccgc gcgacgcgca gcggcagcga gtgctccggc
    18541 ccggggacca gccgccctcc cgcgagcctc caggtgcgca ggcggccggc ctccggggcc
    18601 tggaacagac ccggcgtgcc ggagctccag cgccggagat cgaagctgga cgcttccagc
    18661 aggggacggg gagcggtggg gctatccggc gaccccagcg cccagagctt gcccggctcg
    18721 ccgagcagga tctcttcggc gccgtcgcca tcgaggtctg tcgcggcgag ggtgttggcc
    18781 ttcgcgggaa gcccgtcc
//
LOCUS       DASPJI010000368         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_831146, whole genome shotgun sequence.
ACCESSION   DASPJI010000368 DASPJI010000000
VERSION     DASPJI010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831146"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2493
                     /locus_tag="VEL74_09880"
     CDS             <1..>2493
                     /locus_tag="VEL74_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62878.1"
                     /translation="NLVQGVRLDGALRPGVLAGCFTELTRRHRVLATRYEPTDSGPVQ
                     VIDQGIGSGGPFPLRQVDLSGLPAERRDAEVERVGSREGGRPFDLVRGPVMRATLIRL
                     GPDEHVLLLVIHHIATDGWSMGILLQELAASYPVLAAGGAPSLPAPRLQYADYAVWQR
                     RTLHGEALEAQFAYWRRQLAPPLPVLRLPADRPRVEAQALRLDAEELLLPAALAADLR
                     VLGNARGASLFMVLLAGLTAVLQRHGGDDQILVGAPVAGRNRVEVEGLVGFFLNTLVL
                     RVDLDGDPGFGTLLERSAATALGAFSHQDLPLETLLQELRIAGEGGRSGPFQVMLLLQ
                     NLRPRRFEVPGLALTSLATETQADLGTSIFDLSLTVGDGPGDLRAEMAYNALLFEPAT
                     IRRLLGHLRTLLEGAAAAPATSLSELPLLSPEERREVLAWNPPVEPPAAASIPDLFAA
                     WAARTPAAEAVRHGGRIVTYGELDRMSRRIAERLRVLGVGPEVPVAVLLERSPELIAA
                     LLGVLRAGGVYAPLDPALPGERLAWIAEDLAPAVLVTESALAGRLPWQGRALFLDKAE
                     IPEVPGSPTVPALPEQAAYVIYTSGSTGRPKGVTVPHGALSAFVAAARSLYDIGASDR
                     VLQLASIGFDASVEEIYPCLAAGGTLVLRTDEMAATLDGFLDGCESQRITVLDLPTAF
                     WHELAASPELERRGLPPAVRLVILGGERPLPERVAAWLRSTDPGVRLFNTYGPTEATV
                     VATAAELSPERDGEGWREVSMGRPLGAARAHAVDGALRPVPVGVRAELLLGGPALARG
                     YLGRPDLTAERFLPDPFADLPGARLYRTGDLAR"
BASE COUNT          256 a          941 c          941 g          355 t
ORIGIN      
        1 caacctggtc cagggcgtgc gcctcgacgg cgcgttgcgt cccggcgtgc tggccggttg
       61 cttcaccgag ctgacccgcc ggcaccgggt gctcgccacc cgctacgagc ccaccgattc
      121 cgggccggtg caggtgattg atcaggggat cgggtccggc gggccgttcc ccctgcggca
      181 ggtggacctc tccggcctgc cggcggagcg gcgggacgcg gaggtggagc gggtgggtag
      241 ccgggagggc ggccggccgt tcgacctcgt ccgcgggccg gtgatgcgcg ccaccctcat
      301 ccggctcggc ccggacgagc acgtgctgct gctggtgatc caccacatcg ccaccgacgg
      361 ctggtcgatg ggcatcctgc tccaggagct ggccgcctcg tacccggtgc tcgccgccgg
      421 cggcgccccg tcactccccg cgccgcgcct ccagtacgcg gactacgcgg tctggcagcg
      481 ccgcaccctg cacggcgagg cgctggaggc ccagttcgcc tactggcggc gccagctcgc
      541 gccgccgctg ccggtgctgc ggctgccggc cgaccggccg cgggtggagg cccaggcgct
      601 ccgcctcgac gccgaggagt tgctcctgcc ggccgccctg gccgcggacc tgcgcgtcct
      661 cggcaacgcc cgcggcgcct ccctgttcat ggtcctcctg gccgggctga ccgccgtcct
      721 ccagcgccat ggcggggacg accagatcct ggtgggagcg ccggtggccg gccgcaaccg
      781 ggtggaggtg gaagggctcg tcggcttctt cctcaacacc ctggtgctgc gggtcgacct
      841 ggacggcgat ccgggcttcg gcacgctgct ggagcggtcc gcggccacgg ccctaggggc
      901 gttctcccat caggacctgc cgctggagac cctgctccag gagctgcgga tcgccggcga
      961 gggggggcga agcggcccct tccaggtgat gctgctgttg cagaacctgc ggcccaggcg
     1021 cttcgaggtg ccggggctgg ccctgacctc cctggcgacg gagacgcagg ccgatctggg
     1081 cacgtcgatc ttcgacctca gcctcacggt cggcgacggt ccgggcgatc tgcgcgccga
     1141 gatggcctac aacgccctgc tcttcgagcc cgccaccatc cggcgcctcc tcggccacct
     1201 gcggaccctc ctcgagggcg cggcggcggc cccggcgacg tcgctatccg agctgccgct
     1261 gctgtcgccg gaggagcggc gcgaggtgct ggcctggaat ccgccggtgg agccgccggc
     1321 cgccgcgtcc atcccggatc tcttcgcggc ttgggcggcg cgcacgccgg cggccgaggc
     1381 ggtgcgccac ggcggccgta tcgtcaccta tggcgagctc gaccggatgt cacggaggat
     1441 cgccgagcgg ctgcgcgtcc tgggcgtcgg ccccgaggtg ccggtggcgg tgctcctgga
     1501 gcgctccccc gagctgatcg cggcgctcct gggcgtgctg cgggcgggcg gcgtctacgc
     1561 gccgctcgat cccgcgctcc ccggtgagcg cctggcctgg atcgccgagg acttggcgcc
     1621 cgccgtcctg gtcaccgaga gcgccctcgc cgggcggctc ccctggcagg ggagggcgct
     1681 gttcctggac aaagcggaga tcccggaggt tcccggctct cccacggtcc cggcgctgcc
     1741 ggagcaggcg gcctacgtca tctacacctc cggctccacc ggccgtccga agggggtcac
     1801 ggtgccgcac ggcgcgctgt ccgcgttcgt ggccgcggcg cggtcgctgt atgacatcgg
     1861 cgcctcggac agggtgctgc agctcgcctc gatcggcttc gacgccagcg tggaggagat
     1921 ctatccctgc ctggccgccg gcggcaccct ggtcctgcgc accgacgaga tggccgcgac
     1981 cctggacggc ttcctcgacg gctgcgagtc ccagcggatc accgtgctcg acctgccgac
     2041 ggcgttctgg cacgagctgg cggcgagccc ggagctggag cgccgcggcc tgccccccgc
     2101 cgtgcgcctg gtaatcctcg gcggcgagcg cccgctgccg gagcgggtgg cggcctggct
     2161 gcgaagcacg gacccgggcg tgcgcctgtt caacacctac ggtcccacgg aggcgacggt
     2221 ggtggccacg gcggccgagc tgtcgccgga gcgcgacggc gaggggtggc gcgaggtgtc
     2281 catgggccgg ccgctcggcg ccgcccgggc gcatgcggtg gacggcgcgc tgcggccggt
     2341 gccggtgggg gtgcgggccg agctgctgct cggcggtccc gcgctggcgc gcggctatct
     2401 cggccggccg gacctgaccg ccgagcgctt cctcccggat cccttcgcgg acctgcccgg
     2461 cgcccgcctc taccgcaccg gcgacctggc gcg
//
LOCUS       DASPJI010000369         7735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_424795, whole genome shotgun sequence.
ACCESSION   DASPJI010000369 DASPJI010000000
VERSION     DASPJI010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7735)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424795"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VEL74_09885"
     CDS             complement(<1..451)
                     /locus_tag="VEL74_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62879.1"
                     /translation="MNRSLSRFTLTLLLLTLPLVVVPAGTAQDGGASPTYINRYEIRF
                     LDLHAAELLAWNQCPTKERCKVTSLVSPGEQDKPSRTYLEVSADATVHERIVQALAKE
                     DAAPRTRTFQVVLLLADSKPEGSAPRLPEGAQKALQDIRDLLPFKSYH"
     gene            complement(483..1124)
                     /locus_tag="VEL74_09890"
     CDS             complement(483..1124)
                     /locus_tag="VEL74_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62880.1"
                     /translation="MNEEIRAREERLRAILRQGDPAGGEAGLAQEEVRRVRRAVLAAL
                     PPFGEPRRRAFWMPAVAMTAAAALTLAIALGLWRAHTDPAGPARETGRQAVRVHVPPG
                     PAPVLREAPEEVSPAPVAPSRESALPKAEAPRRPRSRGAVTPAAPPAAPPSLESARLA
                     SASEEGPEVPAVEPDSTLLPEEPAMRQVQFAAPGGTRVLWMLSTESVVDEFAE"
     gene            complement(1124..1669)
                     /locus_tag="VEL74_09895"
     CDS             complement(1124..1669)
                     /locus_tag="VEL74_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG62881.1"
                     /translation="MERTYSSDATERLARLFDAHQERLFRLARRLSTDREEALDLVQE
                     TFLRAAGRPGSVPAGNSAEEAWLVRTLVNLVRDRYRRLSVRSRVHADLRRAGHGASHP
                     EEAAVARATVQAALARLAPRRRAVVVLHELEGVPVSQVARLLGVSEVTVRWHLLAARR
                     ELAKALLPQPSEEVLEERRQR"
     gene            complement(1811..3883)
                     /locus_tag="VEL74_09900"
     CDS             complement(1811..3883)
                     /locus_tag="VEL74_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62882.1"
                     /translation="MSVRAPLLSTLLAVAPLMATAVGAQPSIGADVVRQTVDKLVARH
                     GQEQSGRIRQGVQQVAQRWWKEDGDAQAFTTFCEANFLADPAALGQTFVRLQEVMEQV
                     DGRMLEINRMLTSPSQLDTGPISPLDQLLGNVDLAPHVDEDLFENKVAFLALLNFPVH
                     TLEERLEKGASWDRDTWARSRMMDRFATRVPAEVAQAQSRAFVAADQYIADYNIRMDR
                     LVTPEGQRLFPEGLRLITHWGLRDELASHYAGTGAESLAKQRMILRVMERIVRQEIPA
                     AVIDNPDLLWDPAGNTVRPASPGAGAADKPFDAKTAAAREPDTRYARLLEVFRAVRLA
                     DPYSPTEPTFMERRFERDRQIPEKDVEALLVSVLESAEVKELARLIERRLGRKLEPFD
                     IWYAGFKSRGSYGEEELDKIVQARYPTVESFQAGLPDILQKLGFAPGKARWLADHIVV
                     DPSRGAGHALGAVRREDKAHLRTRIPQGGMNYKGYNIAVHELGHNVEQVFSLNGIDHW
                     WLSGVPNNAFTEALAFVFQNRDLEVLGLKTPGAEARHAEALGTLWNTYEIAGVSLVDM
                     RVWNWMYQHPDATPAELREAALGIARDVWNRYYAPVFGVRDSDILAIYSHMIVYGLYL
                     PDYAIGHIIAFQVADKLEEGNFGTEFERVARQGRLTPDAWMRGAVGGPISAKALLDAA
                     REALRAQN"
     gene            complement(3970..5061)
                     /locus_tag="VEL74_09905"
     CDS             complement(3970..5061)
                     /locus_tag="VEL74_09905"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYG62883.1"
                     /translation="MKPVDINDFLAQRVIGQEEALRYVSVAIFKHLQGEKYGNLLLIG
                     NSGTGKTTIMRAVEALYSAHEELQEHRVVLIMNASTLATEEGAVDTSRLFYRLEERAR
                     QILGPGATPEQIGRTMERATVCLDEIDKISGLIGGKPYVTGINIQQAVLTLIEGERVL
                     HPITGMRDGVAEASSVWVDTGKMLFLCAGAFETLYDQVFARVISPKSGIKLPTTTTYV
                     NGKIQIREYFTLRHHFRQEDLFEYGMQPQFLSRFDNAVILEDLTSATLSRIFTEPEDG
                     VLKSSRSFFQNYGIDLEVTDGAVQKIAEEASKSSRIGARALKSVYGKIIKPFEFDPFS
                     REEVRKNGDDRHRLVIDEPLVSNALKPLV"
     gene            5263..7593
                     /locus_tag="VEL74_09910"
     CDS             5263..7593
                     /locus_tag="VEL74_09910"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HYG62884.1"
                     /translation="MTRSAVHDLERQLNPEQIAAVTHGEGPQLVLAGAGSGKTRVITY
                     RVAWLVEVQGIDPGHIAAMTFTNKAAAEMRERVEDLLGLHPLPASVGTFHRFALVLLR
                     RWGERVGLRRDFAILDGADQLALIKEALSGEGLSESAFSPRAVLHAISAAKNRLIEPP
                     AYESMAQNFFEKKVANLYRRYQGLLQQASGVDFDDLISLSVKLLTEDRDVRERIRFRT
                     RYLMVDEYQDTNHAQLRLVQELAGTDGNITAVGDEDQGIYRWRGADLDNILRFEDTFP
                     GAVVRKLERNYRSTQTILDVSGALIANNVNRRGKRLWTEGEQGVKVVLYRAADEQEEA
                     RWVVDTLQKNRREVRLSEMAVLVRTNAQTRALEEELLRQEVPYSLVGGVRFYDRAEIK
                     DLVAYLRVLRNPRDNFSLMRVVNQPPRGIGKSTLELLRDRAVQLGQPLWDVLYLDDLG
                     TLPARSATALRRFRDLIVGLQRSASELPLPALLDRLLEATAYLDLYKTEDPDDQARLE
                     NIREFLSAAQEFTESISYNSRDEDTLTAFLDHVSLVSDLDSLQDERGVSLMTLHSAKG
                     LEFRTVVVAGLEDGLLPHFNSQGAREDLEEERRLLYVGMTRARERLHLTCCRRRRIAG
                     RYQDQMESPFLRELPDQLLDQRQSPNLFIPETRFQERAQDVYSFFGQGRPAAPAAAPP
                     RTAPAPLPTSARRPAPPSAPSSPSPSYRERTVVPDVQEAPRRPLRRGSRVRHPTLGQG
                     VVLELEGQGEEAKITVFFEKSGKRKLVARFANLEML"
BASE COUNT         1125 a         2741 c         2703 g         1166 t
ORIGIN      
        1 catggtagct cttgaagggc aggaggtcgc ggatatcctg cagggccttc tgcgccccct
       61 ccggcagccg gggagccgag ccctccggct tgctgtcggc cagcagcagc accacctgga
      121 aggtgcgggt gcggggcgcg gcgtcctcct tcgccagggc ctgcacgatc cgctcgtgga
      181 cggtggcgtc cgccgagacc tcgaggtagg tcctggaggg cttgtcctgc tctccggggc
      241 tgacgaggga ggtcaccttg cagcgctcct tggtggggca ctggttccag gcgaggagct
      301 cggcggcatg gagatcgagg aaccggatct catagcggtt gatgtacgtg ggagaggccc
      361 cgccgtcctg cgcggtcccc gcgggcacca cgacgagcgg cagcgtcagg agcaggagag
      421 tcagggtgaa acgagacagc gaacggttca tgtagtttcc tcctgggccg ccgcggcggc
      481 cctcattcgg caaactcgtc gacgacggat tcggtagaga gcatccagag gacccgggta
      541 ccaccgggtg cggcgaactg gacctgccgc atggcgggct cttccggcag cagggtgctg
      601 tccggctcga cagcgggaac ctcgggcccc tcctcggagg cggacgcgag ccgcgccgac
      661 tccagagagg ggggagcggc cggaggagcc gccggtgtca cggcgcctcg cgaccgcgga
      721 cgccggggag cctcggcctt cgggagggcg ctctccctgg agggcgccac gggtgctggg
      781 gaaacctcct cgggtgcctc ccgcagtacc ggggccgggc cgggaggcac atgcaccctg
      841 acggcctgcc ggccggtctc ccgggctggg cccgcggggt cggtgtgagc acgccagagg
      901 ccgagggcga tggcgagggt gagggccgcc gccgcggtca tggccaccgc gggcatccag
      961 aaggcccgcc ggcgcggctc gccgaatgga ggaagcgccg ccaggaccgc gcggcgcacc
     1021 cgccggacct cctcctgtgc cagcccggcc tcgcctccgg ccggatcccc ctggcgcagg
     1081 atggcgcgca gccgctcctc gcgggctcgg atctcctcgt tcatcatcgc tgcctccttt
     1141 cctccagtac ttcctcggac ggctggggga gcagagcctt cgccagctcc cggcgcgccg
     1201 ccagcagatg ccatcgcacc gtcacctcgc tcacccccag cagccgggcc acctggctca
     1261 ccggtacccc ttccagctcg tgcagcacca ccacggcgcg gcggcggggg gcgagccggg
     1321 cgagggccgc ctgcaccgtg gcccgggcca ccgcggcctc ttccgggtga gaggccccgt
     1381 ggccggcgcg ccgcagatcc gcgtgtaccc gcgagcggac ggagaggcgc cggtagcggt
     1441 cgcggacgag gttgaccagg gtccgcacca gccaggcctc ctcggccgag ttccccgccg
     1501 gcaccgagcc cggccggccg gcggcccgca gaaacgtctc ctggaccagg tctagggcct
     1561 cttcgcggtc ggtagagagc cggcgcgcga gccggaacag ccgctcctga tgcgcatcga
     1621 acaaccgggc caggcgctcc gttgcgtctg agctgtaggt tctttccacg gttgcgctcg
     1681 tcgtcagggg tgggttcaca acctgtaaga cgcaccagcc cggaaaaacg ttggggggcc
     1741 cctgaagagg aaaaggccgg cccacccgtc gagagggagg ccggccgctt cgtcttcgcc
     1801 ctgccggttt ctagttctgc gcccgcagcg cctcccgggc cgcgtcgagc agggccttgg
     1861 ccgagatggg gccgcccacg gcgccgcgca tccaggcgtc cggcgtcagg cgcccctggc
     1921 gcgccacgcg ctcgaactcg gtgccgaagt taccctcctc cagcttgtcg gccacctgga
     1981 aggcgatgat gtggccgatg gcgtagtcgg gcaggtagag cccgtagacg atcatgtggg
     2041 aatagatggc gaggatgtcg gagtcgcgga cgccgaagac cggggcgtag tagcggttcc
     2101 agacgtcccg ggcgatgccg agcgccgcct cgcgcagctc cgccggagtg gcgtccgggt
     2161 gctggtacat ccagttccac acccgcatgt ccaccagcga cacgccggcg atctcgtagg
     2221 tgttccacag cgtgccgagg gcctcggcgt gccgggcctc ggcgccgggg gtcttgaggc
     2281 ccaggacctc caggtcccgg ttctggaaga cgaacgccag cgcctcggtg aaggcgttgt
     2341 tgggcactcc cgagagccac cagtggtcga tgccgttgag cgagaagacc tgctcgacgt
     2401 tgtggccgag ctcgtggacc gcgatgttgt accccttgta gttcatgccc ccctgcggga
     2461 tgcgggtccg cagatgggcc ttgtcctcgc gccgcaccgc gccgagcgcg tggccggcgc
     2521 cgcgggacgg gtccaccacg atgtggtcgg cgagccagcg cgccttgccg ggagcgaagc
     2581 ccagcttctg caggatgtcc ggcaggccgg cctggaagga ctcgaccgtc ggatagcggg
     2641 cctggacgat cttgtccagc tcctcctcgc cataggagcc gcgcgacttg aatccggcgt
     2701 accagatgtc gaacggctcg agcttgcggc cgagccggcg ctcgatgagc cgggcgagct
     2761 ccttgacctc cgcggactcg aggaccgaga ccagcagcgc ctccacgtcc ttctccggga
     2821 tctgccggtc gcgctcgaag cgccgctcca tgaaggtcgg ctcggtgggg ctgtacggat
     2881 cggccaggcg caccgcccgg aagacctcca gcaggcgggc atagcgggtg tccggctcgc
     2941 gcgccgccgc cgtcttggcg tcgaacggct tgtccgccgc cccggcgccc ggcgaggccg
     3001 gacggacggt gttgccggcc gggtcccaga gcaggtcggg gttgtcgatc accgccgccg
     3061 ggatctcctg gcgcacgatc cgctccatga cgcggaggat catccgctgc ttcgccaggg
     3121 actcggcgcc ggtccccgcg tagtgcgagg ccagctcgtc gcgcagcccc caatgggtga
     3181 tgagccgcag cccctcgggg aagaggcgct gcccctccgg cgtgaccagc cggtccatgc
     3241 ggatgttgta gtcggcgatg tactggtccg ccgccacgaa ggcgcggctc tgggcctgcg
     3301 ccacctccgc cgggacccgc gtcgcgaagc ggtccatcat gcgcgaccgg gcccaggtgt
     3361 cgcggtccca cgacgcccct ttctccagcc gctcctccag cgtgtgcacc gggaagttga
     3421 ggagcgccag gaaggccacc ttgttctcga acaggtcctc gtcgacgtgg ggggcgaggt
     3481 cgacgttgcc caggagttgg tccagcgggg agatcgggcc ggtgtcgagc tgcgacggcg
     3541 aggtgagcat ccggttgatc tcgagcatcc ggccgtcgac ctgctccatc acctcctgga
     3601 ggcggacgaa ggtctggccg agggccgccg ggtcggccag gaagttggcc tcacagaagg
     3661 tggtgaaggc ctgggcgtcg ccgtcctcct tccaccagcg ctgggccacc tgctggacgc
     3721 cctggcggat gcggccggac tgctcctggc cgtgccgggc gacgagcttg tcgacggtct
     3781 gccgcaccac gtcggcgccg atcgagggct gggctcccac ggcggtggcc atgagcggcg
     3841 ccacggccag gagggtgctc aagagagggg ctcgcacgga catgggatct ccttttcgct
     3901 gccggcgacg ttagcgcatc catggcgggc gcgccagcgg tcgcctacgg cctaaggggg
     3961 tgagggggat cagacgagcg gcttcagggc gttcgagacc agcggctcgt cgatgaccag
     4021 ccggtggcgg tcgtcgccgt tcttgcggac ctcttcccgg gagaaggggt cgaactcgaa
     4081 cggcttgatg atcttgccgt agaccgactt cagggcccgg gcgccgatcc ggctgctctt
     4141 cgaggcctcc tcggcgatct tctgcaccgc gccgtcggtc acctccaggt cgatgccgta
     4201 attctggaag aagctccggg agctcttgag caccccgtcc tccggctccg tgaagatgcg
     4261 ggaaagggtc gcggaggtca ggtcctccag gatcacggcg ttgtcgaagc gcgacaggaa
     4321 ctgcggctgc atgccgtact cgaagaggtc ctcctgccgg aagtgatggc gcagggtgaa
     4381 gtactcgcgg atctggatct tgccgttgac gtacgtcgtc gtcgtgggga gcttgatgcc
     4441 gctcttgggc gagatgacgc gggcgaagac ctggtcgtag agggtctcga aggcgccggc
     4501 gcagagaaag agcatcttgc ccgtgtccac ccagacgctg gacgcctccg ccaccccgtc
     4561 ccgcatgccg gtgatgggat ggagcacccg ctccccctcg atcagggtga ggacggcctg
     4621 ctggatgttg atgccggtga cgtagggctt gccgccgatg aggccggaga tcttgtcgat
     4681 ctcgtccagg cagacggtgg cgcgctccat ggtgcggccg atctgctccg gcgtggcccc
     4741 ggggccgagg atctggcggg cgcgctcctc cagacggtag aagagccggc tcgtgtccac
     4801 cgccccctcc tcggtcgcca gggtgctggc gttcatgatg aggacgaccc ggtgctcctg
     4861 gagctcctcg tgcgccgagt agagggcctc gaccgcccgc atgatggtgg tctttccggt
     4921 gccggagttg ccgatgagca gaagattgcc gtacttctcc ccctggaggt gcttgaagat
     4981 ggccaccgag acgtagcgca gcgcctcttc ctggccgatg accctctggg cgaggaagtc
     5041 gttgatgtcg acgggcttca tggattcctt tctgcgggag cgcctccccg gccccccctt
     5101 cctctatata tacggaagac cggcgccgcg cgccgtctct tcctctctat ataccaggag
     5161 tgcctgccgg ccgctccccg ccctcacaac ctcgtcaacc cccggaagct tcccgcggca
     5221 ctctcccttg ctatccaggc gggggtcgga gaaacttcgc ccgtgaccag aagcgcagtc
     5281 cacgacctcg agcgccagct caacccggag cagatcgccg ccgtcaccca tggtgagggg
     5341 ccgcagctcg tcctcgcggg ggcgggctcg ggcaagaccc gggtcatcac ctaccgcgtc
     5401 gcctggctcg tcgaagtgca ggggatcgac cccgggcaca tcgcggccat gaccttcacc
     5461 aacaaggcgg cggcggagat gcgcgagcgc gtcgaggacc tcctcggcct ccacccgctg
     5521 cccgcctccg tggggacgtt ccaccgtttc gccctggtcc tcctccgccg ctggggggag
     5581 cgggtgggcc tgcgccgcga cttcgccatc ctcgacggcg ccgaccagct cgcgctgatc
     5641 aaggaggcgc tgtccggaga ggggctgtcg gagtccgcct tctccccccg cgccgtgctc
     5701 cacgccatca gcgccgccaa gaaccgcctg atcgagcctc ccgcctacga gtccatggcc
     5761 cagaacttct tcgagaagaa ggtggccaac ctctaccggc gctaccaggg cctgctccag
     5821 caggcctcgg gggtggactt cgacgacctc atctcgctct ccgtgaagct cctcaccgaa
     5881 gaccgcgacg tgcgcgagcg gatccgcttc cgtacccgct atctgatggt cgacgagtac
     5941 caggacacca accacgccca gctccgcctc gtccaggagc tggcgggcac cgacggcaac
     6001 atcaccgcgg tgggcgacga ggaccagggc atctaccgct ggcgcggtgc cgacctggac
     6061 aacatcctgc gcttcgagga caccttcccc ggcgccgtcg tgcgcaagct ggagcgcaac
     6121 taccggtcga cccagaccat cctcgacgtc tccggcgccc tcatcgccaa caacgtgaac
     6181 cggcgcggca agcgcctgtg gaccgagggg gagcagggcg tcaaggtggt gctctaccgc
     6241 gccgccgacg agcaggagga ggcccgctgg gtcgtggaca ccctgcagaa gaaccgccgg
     6301 gaggtccgcc tgagcgagat ggccgtcctc gtccgcacca acgcccagac ccgggcgctg
     6361 gaagaggagc tgctgcggca ggaggtcccc tactcgctgg tcggcggcgt ccgcttctac
     6421 gaccgtgccg agatcaagga cctggtggcc tacctccggg tcctgcgcaa cccgcgcgac
     6481 aacttctccc tgatgcgggt ggtcaaccag cccccccggg gcatcggcaa gtcgaccctg
     6541 gagctgctgc gcgaccgcgc cgtgcagctc ggccagccgc tctgggacgt gctctacctg
     6601 gacgacctgg gcaccctgcc ggcgcggagc gccacggcgc tgcgccgctt ccgcgacctc
     6661 atcgtcgggc tccagcgctc cgcctccgag ctgccgctgc cggccctcct cgaccggctc
     6721 ctcgaagcca ccgcctacct cgacctctac aagacggagg acccggacga ccaggcgcgc
     6781 ctcgagaaca tccgcgagtt cctctccgcc gcccaggagt tcaccgagtc gatcagctac
     6841 aacagccggg acgaggacac cctcaccgcc ttcctcgacc acgtctcgct ggtgagcgac
     6901 ctcgactcgc tgcaggacga gcgcggggtc tccctgatga cgctccacag cgccaagggg
     6961 ctggagttcc ggacggtggt ggtggcgggg cttgaggacg gcctcctccc ccacttcaac
     7021 tcccagggcg cccgcgagga cctcgaggag gagcggcgcc tgctctatgt cggcatgacc
     7081 cgcgcccgcg agcggctcca cctcacctgc tgccggcggc ggcgcatcgc cggccgctac
     7141 caggaccaga tggagtcgcc gttcctgcgg gagctgccgg accagctcct ggaccagcgg
     7201 cagagcccca acctgttcat cccggagacg cgcttccagg agcgggccca ggacgtctac
     7261 tccttcttcg gccagggccg tccggccgcg ccggcggcgg cacccccgag gaccgcgccg
     7321 gccccccttc cgacatcggc gcggcggccg gcgccgccct ccgccccctc ctctccttct
     7381 ccttcctacc gggagcggac ggtggtcccc gatgtccagg aggcgccaag gcgcccgttg
     7441 cggcggggga gccgggtgcg ccatccgacc ctcggccagg gggtggtcct ggagctcgaa
     7501 ggccaggggg aggaggcgaa gatcacggtc ttcttcgaga agtcgggcaa gaggaagctg
     7561 gtggcccgct tcgccaacct cgaaatgctg taaaaaagcg ggacagaccc ccccgactgg
     7621 catcccgccg ccggaggggc ctgccccgct ggggggacag gggggcgggg gctaggtgcc
     7681 gcccgccgag gcctgggacg cggccgcgcc gctcaccagg ttcacgctcg ccgtc
//
LOCUS       DASPJI010000370         7611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_766701, whole genome shotgun sequence.
ACCESSION   DASPJI010000370 DASPJI010000000
VERSION     DASPJI010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766701"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VEL74_09915"
     CDS             <1..207
                     /locus_tag="VEL74_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62885.1"
                     /translation="NAPLYYVRWNLLARTARRDTKYEAPVLGRIEDRTYAAADPLNRA
                     NSANGTERMFRRRIIQTVVNLRNL"
     gene            207..794
                     /locus_tag="VEL74_09920"
     CDS             207..794
                     /locus_tag="VEL74_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62886.1"
                     /translation="MNAREASPRRPHHRPRRKAESGSAYVVALLALLILTFLALALVF
                     VTQTEVQLGSNQRTISRTFFSAETGLAAATARVLTKRGYEPFRFIQNEMQIGNQRMAD
                     EIRVSLVAPIAMEPCDWCPVNDDGFPAFYRVNHAMTIDARRVAWTDNGQVLPPANPDR
                     VLSSRSVSVMYEFQPWQEPPPEAAGTEAQLQQVRF"
     gene            822..5057
                     /locus_tag="VEL74_09925"
     CDS             822..5057
                     /locus_tag="VEL74_09925"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HYG62887.1"
                     /translation="MRRSSQCFQAAVSLALFGLLLLTGGSGAFADDRDLLRESSGEPY
                     VFILLDTSGSMNWTPKCTAAQQAAGECETVCEFGDCYARLQADDPSSKFFQAKEALYE
                     VLKGINDVHFGFATYNQDEMSMRAKHWMYRVRENGVTLPGSGSTPSVRFPAAGAEEVV
                     GYLWGCDTGGGDHEIGCYADDPADLNDSWELARVRRLPKGGRTFSQDVTIYVRHAGTR
                     YRVIYSPDGGGDPGDATLRMDVRVDRCNNSSCSNRTFIGEPTLNFDRATNSLGQAAEF
                     ASWDNGGGSLVRTPTKQDPSVSYFSQGAAADSTASNLCEGWDPNDDTNADSPGYNLRW
                     PTIPGDPRGSLFHYGDVIPMDWQTDHNLDIRRRLAPNTVLDPTADPDFRISSYFRDLP
                     NGSESYLRLKDERARPFLAYGSTPLGNSIRSFRTWYAGCANGNCPKGTGWKHTAAAQD
                     PNWGCRKKFLLILTDGDDTCPGADACSGTASLNAQEGILTYVVAFGVENTSGNRLNCM
                     AANGGTGDPIYPQNKQELISALTQIFGQIREQAAAFASAAVPTVQAEAADKIYVSNFT
                     PLNAESVWNGHIHAFLKPLPRTQDGRPDTTVTCPPEGDPERGSCHLWDAAAEIQEQAP
                     EMADLGPINSIGAAQLRLGMGANQRRVFYSLADDGAIPRDLRLFAPPPGAPPAGTPAA
                     GDLWMDLFDAMRLPSSPAAIRPASALRAKTIIAETLTIKHSTIQMAAGGTEPVSYLLG
                     DTFHADPVIADRPADFLRSSRNLFTRGDDCVEGAASDTGYRCFADKHRRRRKMLVVGS
                     NDGQLHFFDAGTWQPGGASTEGRFNDGTGAELFSYIPRLSLPIIRDLAEDTQHIFGLD
                     STPRIDDVFIDPAHNGTPTAADRQWRTVVIGGYREGGDPIGGSRMADFVSGYYALDIT
                     QPDPIEKTHEEGIGPDGLPRQVRWEPETNDSAVPGCLTTNGSIPSGCGPAPFPALLWE
                     FTDSIGASQLDEDRNGFADLGQTWAAPTIGRIRVIESGNVVDKFVAFFSGGMDADSKA
                     NPRRGNYLYMVDVETGDVIYKRALLGATPGEPAVVDTNSDGVLDKVYVATTAGLLYKV
                     DLSTPAELRSHVILRTQALPNLAADTVVQRVTDSLWEPFAIFDTIDRPIYYAPTALFI
                     SRLNTVALAFGTGDRENLWEPAGNQEGRFYLIVDDGFTAAQAASGVLPRDESFYQAVL
                     PGDLKTNKDYVLNPEPGKSRGWFLRLDPGERVITPAFGLVGFLTFSSYEPQVTVSGGG
                     GGAVCGRTGVSRVYVIFASNANGVLRGPTAGTTQRYSEVPVFLAPPTIDSGPTQNRPR
                     GGPGSGPGGGPGGGPGGGGPSGGTTDHDGDGFEDTLTLDQREIMEELKKYFPDGTRFG
                     HYWYDVSAMGSDTRYVGIAAIPIGILQKNWKEN"
     gene            5063..5830
                     /locus_tag="VEL74_09930"
     CDS             5063..5830
                     /locus_tag="VEL74_09930"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HYG62888.1"
                     /translation="MKSRRPALLGALGFSALLVLPGTVGADWLVTREGARMEIQGHWQ
                     VKGKLVVFTRTDGSLASLRLSEVDLEASERATAAAMEAAVAAETEEATRQTAPEPEKK
                     KSKFVLTDADFRKAPPPPATQEEGGEAQTVTPEGQDVGEAGSQEKVVVGSWRQAARTE
                     GDGLDVFGVLQNTGNELATDVAVTVELFAETGEQVGTSQAVLSAPGIRPNASITFRAA
                     FPNVFTFSEARFNVRSRSLTLAPLPAEGEERETQAQP"
     gene            complement(5886..7223)
                     /locus_tag="VEL74_09935"
     CDS             complement(5886..7223)
                     /locus_tag="VEL74_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYG62889.1"
                     /translation="MPTGRILIVEDRDSLRRLLERALSQEGYEVLSAADGKEGIRLAR
                     ESPVDLVLTDLMLPEASGLEVLAASRAAQPRVPVVVLTGFGTVGTAVEAMKLGAFDFL
                     EKPVELDDLARLVERALAEKGDRGEKGDDAALRAPGAPPIIGRHPRLRAAVRLAEKVA
                     PTESTVLLTGESGTGKELFARAIHALSPRRGGPYVAINCAAIPEALLENELFGHEKGA
                     FTGADRRQQGRFEFAEGGTLFLDEIGELPLAVQGKVLRVLEERTFERVGSGRTQRADI
                     RLVAATNRDLARMVEEGTFRSDLFFRLNVFPIELPALRERASDVPLLARHLLSEIARR
                     HRLEPPRLEEDAAELLSTQAWPGNVRELGNVLERALILAEAPALRAHDLRPLLQPAAG
                     ATTAAGERDRLRQALIETDGDKRRAALLLGMGYRTLLRKVKEHDLEGVPRYRE"
     gene            complement(7242..>7611)
                     /locus_tag="VEL74_09940"
     CDS             complement(7242..>7611)
                     /locus_tag="VEL74_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HYG62890.1"
                     /translation="VLRLHLAGAELPIDRLWPDPRAHRARLESAAAEINAGGDPVAAV
                     ATIVPADAFLGGGSAPEEPIPGEALALPGDDELLARLRRGDPPVVGYIRRGRLLLDLR
                     TVAPEDDEALVDAVRRALAS"
BASE COUNT         1151 a         2814 c         2613 g         1033 t
ORIGIN      
        1 aacgcgccgc tctactacgt gcggtggaac ctgctcgccc gcaccgcccg ccgcgatacg
       61 aagtacgagg cgcccgtcct gggccgcatc gaggaccgca cctacgcggc cgccgacccc
      121 ctgaatcggg ccaactccgc caacggcacc gagcgcatgt tccggcgccg catcatccag
      181 accgtggtga acctgaggaa cctctgatga atgctcgcga agcttcgccg cgccgtccgc
      241 atcaccgtcc gcgccgaaaa gcggagtccg gcagcgccta cgtggtcgcc ctgctcgccc
      301 tgctgatcct caccttcctg gccctggccc tggtgttcgt cacccagacc gaggtccagc
      361 tcggctccaa ccagcgcacc atcagccgca ctttcttctc ggccgagacc ggcctcgccg
      421 cggcgaccgc ccgggtgctc accaagcggg gctatgagcc cttccgcttc atccagaacg
      481 agatgcagat cggcaaccag cggatggccg acgagatccg ggtgtcgctg gtggcgccga
      541 tcgccatgga gccctgcgac tggtgcccgg tcaacgacga cggcttcccc gcgttctacc
      601 gggtcaacca cgccatgacc atcgacgccc ggcgcgtcgc ctggaccgac aacggccagg
      661 tgctgccgcc ggccaacccc gaccgggtgc tgtcgagccg ttcggtgagc gtgatgtacg
      721 agttccagcc ctggcaggag ccgccgcccg aggcggccgg caccgaagct caattgcagc
      781 aggtccgatt ctgatccgtt actttgttgg aggagatcat catgcgccgt tcctcccagt
      841 gttttcaggc cgccgtctcg ctggccctgt ttggactgct gctcctgacc ggcgggtccg
      901 gagcgttcgc cgacgaccgt gacctgctgc gggagagcag cggcgagccg tacgtcttca
      961 tcctcctgga cacctccggc tccatgaact ggacgccgaa gtgcaccgcc gcccaacagg
     1021 ctgccggcga gtgcgaaacc gtctgcgagt tcggcgactg ctacgcccgt ctccaggcgg
     1081 acgatccttc gtccaagttc ttccaggcga aggaggcgct gtacgaggtg ctcaagggca
     1141 tcaacgacgt gcacttcggc ttcgccacct acaaccagga cgagatgagc atgcgggcga
     1201 agcactggat gtaccgcgtg cgcgagaacg gcgtgaccct gccgggcagc ggctcgaccc
     1261 cgagcgtccg cttcccggcc gccggggccg aggaggtcgt cggctacctc tggggctgcg
     1321 ataccggcgg cggcgaccat gagatcggct gctacgcgga cgatccggcc gacctcaacg
     1381 actcctggga gctcgcccgc gtccgccgcc tgcccaaggg gggccggacc ttcagccagg
     1441 atgtgacgat ctatgtccgc cacgccggca cccggtaccg cgtgatctac agccccgacg
     1501 gcggcggcga tccgggcgac gcgaccctgc gcatggacgt gcgggtggac cggtgcaaca
     1561 acagctcctg ctccaaccgc accttcatcg gtgagccgac ccttaacttc gaccgggcca
     1621 ccaactccct gggccaggcg gccgagttcg cctcctggga caacggcggc ggctcgctgg
     1681 tccgcacccc caccaagcag gatccgtccg tctcctactt cagccaggga gcggcggccg
     1741 acagcaccgc cagcaacctc tgcgagggct gggacccgaa cgacgatacc aacgcggaca
     1801 gccccgggta caacctgcgc tggccgacga tccccggcga cccccgcggc tcactgttcc
     1861 attacggcga cgtcatcccg atggactggc agaccgacca caacctggac atccggcggc
     1921 ggctggcccc gaacacggtg ctcgacccga cggccgaccc ggacttccgg atcagctcct
     1981 acttccggga cctgccgaac ggcagcgaga gctacctgcg gctcaaggac gagcgggcgc
     2041 gcccgttcct cgcctacggc tcgacgccgc tcggcaactc gatccggtcg ttccgcacct
     2101 ggtacgccgg ctgcgccaat ggcaactgcc ccaaggggac cggctggaag cacaccgccg
     2161 ccgcccagga cccgaactgg ggctgccgca agaagttcct gctgatcctc accgacggtg
     2221 acgacacctg ccccggcgcc gacgcctgct ccggcacggc gtccctcaac gcccaggagg
     2281 ggatcctcac ctacgtggtg gcgttcggcg tcgagaacac ctcgggcaac cggctgaact
     2341 gcatggcggc caacggcggc accggggacc cgatctaccc gcagaacaag caggagctga
     2401 tcagcgccct gacccagatc ttcggccaga tccgcgagca ggccgcggcc ttcgcctcgg
     2461 ccgcggtgcc gacggtccag gccgaggccg cggacaagat ctacgtctcc aacttcacgc
     2521 cgctcaacgc cgaatcggtc tggaacggcc acatccacgc cttcctgaag ccgctgccgc
     2581 ggacccagga cggccggccc gacaccacgg tgacctgccc gcccgagggg gaccccgagc
     2641 gcggcagctg ccatctctgg gacgcggcgg ccgagatcca ggagcaggca cccgagatgg
     2701 ccgacctcgg tcccatcaac agcatcggcg cggcgcagct ccgtctcggc atgggggcga
     2761 accagcggcg cgtcttctac agcctggccg acgacggcgc gatcccccgc gacctgcggc
     2821 tgttcgcgcc gcctccgggc gcgccgcccg ccggcacacc ggccgccggc gacctctgga
     2881 tggacctgtt cgacgccatg cgcctgccct cgtcgccggc cgccatccgg ccggcctcgg
     2941 cgttgcgggc gaagaccatc atcgccgaga cgctcaccat caagcactcc accatccaaa
     3001 tggcggccgg aggcaccgag ccggtgagct acctgctggg cgacaccttc cacgccgacc
     3061 cggtgatcgc cgaccggcct gccgacttcc tgcgctcgtc gcgcaacctg ttcacccgcg
     3121 gcgacgactg cgtcgagggg gcggcgagcg acaccggcta tcgctgcttc gcggacaagc
     3181 accggcggcg ccgcaagatg ctggtggtgg ggagcaacga cggccagctc cacttcttcg
     3241 acgccggcac ctggcagccc ggcggcgcca gcacggaggg gcgcttcaac gacggcacgg
     3301 gcgcggagct gttctcctac atcccgcggc tgtccctgcc catcatccgc gacctcgccg
     3361 aggacacgca gcacatcttc ggcctggaca gcacgccgcg catcgacgac gtgttcatcg
     3421 atccggcgca caacggcacc cccaccgccg ccgaccgcca gtggcggacg gtggtgatcg
     3481 gcggctaccg ggaagggggc gaccccatcg gcggctcgcg catggcggac ttcgtcagcg
     3541 gctactacgc gctggacatc acccagccgg accccatcga gaagacgcac gaggagggga
     3601 tcggccctga cggcctgccg cggcaggtcc ggtgggagcc ggagaccaat gattcggcgg
     3661 tgccgggctg cctgaccacg aacggctcga tcccgtcggg ctgcggcccc gctcccttcc
     3721 cggcgctgct ctgggagttc accgactcga tcggcgcctc gcagctcgac gaggaccgca
     3781 acggcttcgc cgacctcggg cagacctggg ccgctcccac catcgggcgc atccgggtga
     3841 tcgagtccgg caatgtggtc gacaagttcg tcgccttctt cagcggcggc atggacgccg
     3901 actccaaggc caacccgcgg cgcggcaact acctctacat ggtggacgtc gagacgggcg
     3961 atgtgatcta caagcgcgcc ctcctcggcg cgacgccggg cgagccggcg gtggtcgaca
     4021 ccaactccga cggcgtgctc gacaaggtct acgtcgccac cacggcgggc ctcctctata
     4081 aggtggacct ctccacgccg gcggagctgc gcagccacgt catcctgagg acccaggcgc
     4141 tgcccaacct ggcggccgac acggtggtgc agcgcgtgac cgactcgctt tgggagccgt
     4201 tcgccatctt cgacaccatc gaccggccca tctactacgc gccgacggcg ctcttcatct
     4261 cgcgcctcaa caccgtggcg ctggccttcg gcaccggtga ccgcgagaac ctgtgggagc
     4321 cggcgggcaa ccaggaaggc cgcttctacc tcatcgtcga cgacggcttc acggcggccc
     4381 aggcggccag cggcgtcctg ccgcgggacg agagcttcta ccaggcggtc ctgccgggcg
     4441 acctcaagac caacaaggac tacgtgctca accccgagcc gggcaagtcg cgcggctggt
     4501 tcctgcgcct cgaccccggc gagcgggtga tcaccccggc cttcggcctg gtgggcttcc
     4561 tgaccttctc gagctatgag ccgcaggtga cggtgtccgg cggcggcggc ggcgcggtct
     4621 gcggccgtac cggcgtcagc cgcgtctacg tcatcttcgc gagcaacgcc aatggcgtgc
     4681 tgcgcggacc gaccgccggc accacccagc gctactcgga ggtgccggtg ttcctggcgc
     4741 cgcccaccat cgactcgggt ccgacccaga accggccgcg cggcggcccg ggctcgggcc
     4801 ccggcggcgg tcccggtggt ggtcccggcg gtggtgggcc ctcgggcggc accaccgatc
     4861 acgacggtga cggtttcgag gacacgctca ccctggatca gcgggagatc atggaagagc
     4921 tcaagaagta cttccccgac gggacccgct tcggccacta ctggtacgac gtgagcgcga
     4981 tgggatcgga cacccgctac gtgggcatcg ccgccatccc catcggcatc ctgcagaaga
     5041 actggaagga gaattgatgg tcatgaagtc gcgacgtccg gcgctcctgg gcgccctggg
     5101 attttcggcc ctgctggtcc tccccgggac cgtgggggcc gattggctgg tgacccggga
     5161 gggggcgcgg atggagatcc aggggcactg gcaggtgaag ggaaagctcg tggtcttcac
     5221 ccggacggac gggagcctgg cctcgctgcg gctctccgag gtggatctgg aggcgagcga
     5281 gcgggccacg gccgcggcca tggaggccgc ggtggcggcc gagaccgagg aggccacccg
     5341 gcagacggcg cccgagccgg agaagaagaa gtccaagttc gtcctcaccg acgccgactt
     5401 ccgcaaggcg ccgcctcccc ccgccaccca ggaggagggt ggggaggccc agacggtcac
     5461 ccccgagggg caggatgtgg gggaggcggg gagccaggag aaggtggtcg tgggctcctg
     5521 gcgccaggcc gcacgcaccg agggggacgg tctggacgtc ttcggcgtgt tgcagaacac
     5581 gggcaacgag ctggccaccg acgtggcggt gacggtggag ctgttcgcgg agaccggcga
     5641 gcaggtgggg accagccagg ctgtgctgag cgcgccgggc atccgcccca acgcctccat
     5701 caccttccgc gccgccttcc cgaacgtctt caccttctcc gaggcgcgct tcaacgtccg
     5761 gagccgcagc ctcaccctgg cgccgctgcc ggccgagggc gaggagcggg agacccaggc
     5821 gcagccgtag aggctaaccc gtcccccgtc ccccgtcccc cggcctcggg ccgggggacg
     5881 gcccctcact ccctgtaccg cggcaccccc tccaggtcgt gctccttcac cttgcgcagc
     5941 agggtgcggt agcccattcc caggagcagg gcggcgcggc gcttatcgcc gtcggtctcg
     6001 atgagggcct ggcggaggcg gtcgcgttcg ccggctgcgg tggtcgcccc ggccgcgggc
     6061 tggagcaggg ggcggaggtc gtgagcgcgc agcgccggag cctcggcgag gatcagcgcg
     6121 cgctccagga cgttccccag ctcccggacg ttgcccggcc aggcctgggt ggagaggagc
     6181 tccgcggcat cctcctcgag ccgcggcggt tccagacggt gccggcgggc gatctcggag
     6241 aggagatggc gggcgagcag cggcacgtcg ctcgcccgct cccgcagcgc cggcagctcg
     6301 atcggaaaga cgttcagccg gaagaagagg tcggaccgga aggtgccctc ctcgaccatc
     6361 cgcgcgaggt cccggttggt ggccgccacg agccggatgt cggcgcgctg cgtccgcccg
     6421 ctccccacgc gctcgaaggt ccgctcctcc aggacgcgca gcaccttgcc ctggaccgcc
     6481 agggggagct cgccgatctc gtccaggaag agggtgcccc cctcggcgaa ctcgaagcgg
     6541 ccctgctgcc ggcggtcggc gccggtgaag gcccccttct cgtgaccgaa cagctcgttc
     6601 tcgaggagcg cctcggggat cgccgcgcag ttgatcgcca cgtacggccc gccgcggcgg
     6661 ggcgacagcg cgtggatcgc ccgggcgaac agctccttgc ccgtgccgct ctcgccggtc
     6721 agcagcaccg tgctctccgt cggcgccacc ttctccgcca ggcggacggc ggcgcgcagc
     6781 cgcgggtggc ggccgatgat gggcggcgct cccggcgcgc gcagcgccgc gtcgtccccc
     6841 ttctccccgc ggtccccctt ctctgccagc gcccgctcca cgagacgggc gaggtcgtcc
     6901 agctccaccg gcttctccag gaagtcgaag gcgcccagct tcatcgcctc gaccgcggtg
     6961 cccaccgtgc cgaagccggt gaggaccacc accggcaccc gcggctgggc cgcgcggctg
     7021 gcggcgagga cctccaggcc cgatgcctcc gggagcatca ggtcggtgag cacgaggtcc
     7081 accgggctct cccgggcgag gcggatcccc tccttcccat cggcggcgga gaggacctcg
     7141 tacccctcct gggagagggc gcgctccagc aggcggcgga gcgagtctcg gtcctccacg
     7201 atcaggatgc ggccggtggg catggcgggg tggctcggag gtcaggaggc gagggcgcgg
     7261 cgcactgcgt ccacgagggc ctcgtcatcc tcgggggcga cggtgcggag gtcgaggagc
     7321 agccggccgc ggcggatgta gcccacgacc gggggatctc cccggcgcag gcgggccagg
     7381 agctcgtcgt cgccggggag cgccagcgcc tcgccgggga tgggctcctc gggcgccgag
     7441 ccgccgccga ggaaggcgtc ggccggcacg atcgtggcca ccgcggccac gggatcgccg
     7501 ccggcgttga tctcggccgc ggcgctctcc aggcgggcgc ggtgggcccg cgggtcgggc
     7561 cagagacggt cgatggggag ctcggcgccc gcgaggtgca ggcggaggac c
//
LOCUS       DASPJI010000371         8626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_593349, whole genome shotgun sequence.
ACCESSION   DASPJI010000371 DASPJI010000000
VERSION     DASPJI010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8626)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593349"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VEL74_09945"
     CDS             complement(<1..400)
                     /locus_tag="VEL74_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62891.1"
                     /translation="MDTLRRHIETWHSPALGQSMPIVSYGHWGHPLLLFPTAAADFLE
                     NERFGLVGAVGQAIDEGRVRVFSINSINNQSWMDRGLPVPEKGRRQALYNQYIEGEVV
                     PYIRSVCRSGDIRIATAGASFGAFHAANTLF"
     gene            459..1250
                     /locus_tag="VEL74_09950"
     CDS             459..1250
                     /locus_tag="VEL74_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYG62892.1"
                     /translation="MERIAILEEGLENGREVSHLAVRYDEPPGGGSLCLLYLHGFGSD
                     QWGEKAEFFRDRALAAGFGFCSLDFQGHGRSGGTMQGLTLSRNLDDVARVHAWLRERG
                     AVDENGRLVLVGSSMGGGTALWYSACHPEGIAAGLHIAPALDLERSLLAWAGPERTRT
                     WQETGAIHFENEFVSCELSWELIEDLRRHPMEDLLASYRTPALLLQGKKDASVPWKSV
                     ADFAVRVPFEEVELHLFADGDHRLTDRKHRLWDLMLEFLRGRALA"
     gene            complement(1293..2102)
                     /locus_tag="VEL74_09955"
     CDS             complement(1293..2102)
                     /locus_tag="VEL74_09955"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HYG62893.1"
                     /translation="MLRNKLRVLLCLCAFLPVVATLAACGGGTEEGTPEAAQAPAGPP
                     VDLTDAGGVKGVIRYTGPDTDTRLPLEADPVCSGLHKDGPVDSGAVVAKDGQLANAFV
                     YVKSGLEGKSFPVPAEAKLLDQQGCIYTPRVLGVQVGQTLKIKNSDPTLHNVSALPKV
                     NGEFNESQPIPGMEFDKKFDKPEVMVHFKCNVHPWMTAFVGVLPHPYFAVTGENGAFD
                     LAKLPPGTYTLEAWHESLGTQTQQVTVAPNQTAEVTFEFPAAASPAAATGG"
     gene            complement(2208..3662)
                     /gene="moeB"
                     /locus_tag="VEL74_09960"
     CDS             complement(2208..3662)
                     /gene="moeB"
                     /locus_tag="VEL74_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013051199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HYG62894.1"
                     /translation="MAVTIHIPTPLRRFTGAHGEVRADGMTVGEALRDLVRQHPAIER
                     HLYADDGSLRSFVNLFLNDEDVRHLQRGDTPLSSGDTLMIVPSIAGGAGAALEGKAEE
                     VAAEELTPAEIRHYSRHLIMPEIGPDGQKRLKAAKVLMVGAGGLGSPLGLYLAAAGVG
                     TLGIVDFDVVDESNLHRQVLYGAQDVGRPKLHAAIERLRGVNPHIRLVPHEMRLDSGN
                     ALDLCAGYDIVVDGTDNFPTRYLVNDACVLLGKPNVFGSIFRFEGQVSIFWGARGPCY
                     RCLFPEPPPPGLVPSCAEGGVLGVLPGIIGSLQANEVIKLIAGVGDPLIGRLLLFDAL
                     KMKFRELKLRKDPGCPVCSEHPTQTGLIDYNQFCGIPQEQARVEDDFEMSVTDLKRWM
                     DEGRDFKILDVRTPQEHQIAHLPGTRLIPLQELPGRLGELDPADTLVVHCHHGGRSAQ
                     AVELLRRKGFAKSINLAGGIHAWSEEIDPSVPRY"
     gene            complement(3662..4141)
                     /locus_tag="VEL74_09965"
     CDS             complement(3662..4141)
                     /locus_tag="VEL74_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HYG62895.1"
                     /translation="MNRLVITPRHLHTIGRIASMTYPEECCGVLVGRPQGDSTIVERI
                     LTVQNERQESRHNRYLISPETVLAAQKEARALGLDIVGYFHSHPDHPARPSEFDREHA
                     WPGYSYLIVSVQNKKVMEARSWRLSDDRERFDEEAIDQAAGAPIPGYLPMGVHREAV"
     gene            complement(4330..5154)
                     /locus_tag="VEL74_09970"
     CDS             complement(4330..5154)
                     /locus_tag="VEL74_09970"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYG62896.1"
                     /translation="MFRRPQRSGSILCPSCGRLVGVNDEVCLNCGRRNPGMWGLTSVL
                     GKLGRQDLGFEKAVIGGSVLLYLLMLVVTPGFGLGGGMGPASLLAPSQEAMLRFGASG
                     AIPVLGHGRWWTLLSAGWLHAGILHILFNVMWVRQLAPQTAELYGASRMVILYTVSSV
                     CGFLASTLLMTPITLGASAPIFGLLGALVCYGRRAGSSAIGQQAWTYAIILFAFGFIF
                     RGVDNWAHLGGFVGGYLTALVLDPRKGERPGHTILAIGCLAATALSILASLVVPLR"
     gene            complement(5188..5424)
                     /locus_tag="VEL74_09975"
     CDS             complement(5188..5424)
                     /locus_tag="VEL74_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62897.1"
                     /translation="MEIDRPDQTPAIDPPLDPYDERPDRDPREPVDPPNVEEPQEDPD
                     EPLQDEPIEDPLDAPGVGEPERRDPDPGEPPMQV"
     gene            5510..6001
                     /locus_tag="VEL74_09980"
     CDS             5510..6001
                     /locus_tag="VEL74_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62898.1"
                     /translation="MAPPDSSEWLLQLFWLLILAVPIACVAWTVTHEEIFREPREYCV
                     EKSKNAPSLLQRKFFYLFTCEYCFSHYVTVFFLFVTRYKLLLDDWRGYLLSFFALVAV
                     ANVYMSAFGRLRQEVKSEKLEAKRKEKVLERVERQAEQEKEEQEQEQENGRVVPVPVG
                     QRR"
     gene            complement(6009..6548)
                     /locus_tag="VEL74_09985"
     CDS             complement(6009..6548)
                     /locus_tag="VEL74_09985"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HYG62899.1"
                     /translation="MTVATFAYPWEAELARARLDAEGVESVIADEHLSRLYGAGIVGG
                     VKLRVRREEADRASALLRELRPIPEIYLVKPEDTGAMDEPEEAEEMEDGEERIGDLVT
                     VARFTTPWEAHLARTRLESEGIEACVLEERLPPVNLFTGEILALNRLAVNESDAGRAR
                     EILDRPGLLEDDLDESLAD"
     gene            complement(6633..6821)
                     /locus_tag="VEL74_09990"
     CDS             complement(6633..6821)
                     /locus_tag="VEL74_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62900.1"
                     /translation="MAVPLTIYVLFIVLSMVGGYLAAAHHHPRGRAAGVWALVIIFLF
                     YTGLTLGLYLLLRSGGHA"
     gene            complement(6837..>8626)
                     /locus_tag="VEL74_09995"
     CDS             complement(6837..>8626)
                     /locus_tag="VEL74_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HYG62901.1"
                     /translation="GLPEVQLGLIPGLGGTQRLPRLIGVPDALDLILTGKQVDAKKAR
                     RLGLVDDTCHPSDLREAAQRWVGLGRKGGKELKGIEKKRKAGRSLPMRAGDLLAHTPL
                     VADRFVWEKARAGVMEKTGGHYPAPLVAISVVRDGLKLPLRRALDVEAGAFSELVLSD
                     TAKSLMSIFFMKNEVEARAGRIARQGRPVDVVGILGAGFMGSGIAQVLAYKDMPIVLK
                     DKDLDALGRGMKHCGEQLNELKKRRRLSDAEARRAMARIIPTVDYHDFRGVDFVVEAV
                     FEDVGIKHTVIRETEAVAPDNLIFASNTSTIPIARLAEGSSRPENVVGMHFFSPVAKM
                     PLLEVVRHPGTSDEALGTAVEIGRTMGKTVIVVNDGPGFFTTRVLAPFLNEAAWLLVE
                     GARVDKVDKVMREWGWPVGPFELMDEVGLDIGKHVGEILLAYKGERFSPPAVFARMID
                     DGRLGRKAKKGFYLYGDEDGKKKKGGEGSRKAKKVDEAVYRLLDWRESPVDGREVVER
                     CWMQMLNETARCMEEGIITNPADVDIGVIFGFGFPPFRGGLLHEADRLGLRYVVDRLD
                     AYAAIHGQRLEPAPLLRQMAEKGETFYKD"
BASE COUNT         1319 a         2904 c         3051 g         1352 t
ORIGIN      
        1 ggaacagggt gttggcggcg tggaaggcgc cgaagctcgc gccggcggtg gcgatccgga
       61 tatcgcccga gcggcagacg ctccggatgt agggcaccac ctcaccttcg atgtactggt
      121 tgtagagggc ttggcggcgc cccttctcgg gcactggcag gccgcggtcc atccacgact
      181 ggttgttgat gctgttgatc gagaagacgc ggacccggcc ctcgtcgatg gcctggccga
      241 cggccccgac gagcccgaag cgctcgttct ccaggaagtc cgccgcggcc gtagggaaca
      301 gcagcagggg gtgcccccag tggccgtagg agacgatggg catggattgg cccagcgccg
      361 ggctgtgcca ggtttcgata tggcgtctca gggtgtccat gggcgtcggc ctctctcggt
      421 caaaggtgcg ccatcatatc gcgacgagag gaggacgcgt ggaacggatc gccatcctgg
      481 aagagggttt ggaaaacggc cgtgaggtct cccacctcgc cgtccgctac gacgagccgc
      541 cgggaggggg aagtctctgc ctcctctacc tgcacggctt cggctccgac cagtgggggg
      601 agaaggccga gttcttccgg gaccgcgccc tcgccgccgg tttcggcttc tgctcgctcg
      661 acttccaggg gcacggccgc tccggcggca cgatgcaggg gctgacgctg tcgcgcaacc
      721 tggacgacgt cgcccgggtc cacgcctggc tgcgcgagcg cggcgccgtc gatgaaaatg
      781 ggcgcctggt gctcgtcggc tcctcgatgg gaggcggcac ggccctctgg tactccgcct
      841 gccacccgga agggatcgcc gccggcctcc acatcgcgcc ggccctcgac ctcgagcggt
      901 cgctgctcgc ctgggcgggg ccggagcgga cccggacctg gcaggagacg ggggccattc
      961 acttcgagaa cgaattcgtc tcctgcgagc tgagctggga gctgatcgag gacctccgcc
     1021 gccaccccat ggaggatctg ctcgcctcct accgcacccc cgcgctcctc ctccagggca
     1081 agaaggacgc ctcggtcccc tggaagagcg tggccgactt cgccgtccgc gtcccctttg
     1141 aagaggtgga gctccacctc ttcgccgacg gcgaccaccg gctcaccgac cgcaagcacc
     1201 ggctatggga tctgatgctg gagttcctgc ggggccgggc actggcgtga agccccctct
     1261 cccgcgatgg ggagaggggg gacaaaggcg gcctagccgc cggtcgcggc ggccggcgag
     1321 gccgcggccg ggaactcgaa ggtgacctcg gcggtctgat tcggcgccac cgtcacctgc
     1381 tgcgtctggg tgccgagcga ctcgtgccag gcctccaggg tataggtgcc cggcggcagc
     1441 ttcgccaggt cgaaggcgcc gttctcgccc gtcaccgcga agtaggggtg gggcagcacg
     1501 cccacgaagg cggtcatcca ggggtggacg ttgcacttga agtgcaccat cacctcgggc
     1561 ttgtcgaact tcttgtcgaa ctccatgccc gggatcggct gcgactcgtt gaactcgccg
     1621 ttcaccttgg ggagcgccga gacgttgtgg agcgtggggt cgctgttctt gatcttgagc
     1681 gtctggccga cctgaacgcc caggacccgc ggggtataga tgcagccctg ctggtcgagc
     1741 agcttcgcct cggccggcac cgggaacgac ttcccctcga ggccgctctt cacgtagacg
     1801 aaggcgttgg ccagctggcc gtccttggcg acgacggcgc ccgagtcgac cgggccgtcc
     1861 ttgtggagcc cggagcagac cgggtcggcc tccaggggga gccgggtgtc ggtgtccggg
     1921 ccggtatacc ggatcacgcc cttgacgccg ccggcgtcgg tcaggtccac cggcgggccg
     1981 gcgggcgcct gggccgcttc gggggtcccc tcttcggtgc cgccgccaca ggctgccagc
     2041 gtggccacga ccgggagaaa ggcgcagaga cagaggagta cccgcagttt gttcctcaac
     2101 atttcttcga gaacctccct gaacaatctt gggatcggga atgagcttca cagggccggc
     2161 cgggaggccg gcgcgccatt attgcacagc gcttcgggag gacccgatca gtagcgcggc
     2221 accgacggat cgatctcctc gctccaggcg tggataccgc cggccaggtt gatcgacttg
     2281 gcgaagccct tgcggcgcag gagctcgacg gcctgggcgg agcggccacc gtgatgacag
     2341 tggacgacca gggtgtccgc cgggtcgagc tcccccagcc ggcccggcag ctcctggagc
     2401 gggatcagcc gggtgcccgg caggtgggcg atctggtgct cctgcggcgt gcgcacgtcg
     2461 aggatcttga agtcgcgtcc ctcgtccatc cagcgcttga ggtcggtcac ggacatctcg
     2521 aagtcgtctt ccacgcgcgc ttgctcctgg ggtatgccac agaactggtt gtagtcgatg
     2581 agccccgtct gcgtcgggtg ctcggagcag accgggcagc cggggtcctt gcgcagcttc
     2641 agctcgcgga acttcatctt gagggcgtcg aacagcaaca ggcggccgat caggggatca
     2701 cccacgccgg cgatcagctt gatgacctcg ttcgcctgca gcgagccgat gatgcccggc
     2761 aacaccccca ggacgccccc ctcggcgcag gacggcacca ggcccggcgg cggcggctcc
     2821 gggaacagac agcggtagca gggcccccgc gctccccaga agatggagac ctgcccctcg
     2881 aagcggaaga tcgagccgaa gacgttgggc ttgcccagca ggacgcaggc gtcgttgacc
     2941 aggtaccggg tcgggaagtt gtccgtgccg tcgacgacga tgtcgtagcc cgcgcagagg
     3001 tccagggcgt tgccggagtc gagccgcatc tcgtgcggga cgagccggat gtgcgggttc
     3061 accccccgca ggcgctcgat cgcggcgtgc agcttcggcc gtcccacgtc ctgtgcgccg
     3121 tagagcacct ggcggtggag gttgctctcg tccaccacgt cgaagtccac gatccccagg
     3181 gtgccgacgc cggccgcggc caggtagagg cccagcggcg aaccgaggcc gccggcgccg
     3241 accatcagca ccttggccgc cttgagccgc ttctgcccgt cggggccgat ctccggcatg
     3301 atgaggtgcc ggctgtagtg gcggatctcc gccggtgtca gctcctccgc cgccacctcc
     3361 tccgccttcc cctccagagc ggcgccggcc ccgcccgcga ttgacgggac gatcatcagc
     3421 gtgtcgccgg acgagagcgg ggtgtccccc cgctggaggt ggcgcacgtc ctcatcgttc
     3481 aggaacaggt tgacgaagct gcgcaacgat ccgtcatccg cgtagaggtg ccgctcgatg
     3541 gcggggtgct ggcggacgag gtcgcgcagc gcctccccca ccgtcatccc gtcggcccgc
     3601 acctcgccgt gcgcgccggt gaagcggcgc agcggcgtcg ggatgtggat cgtcacggcc
     3661 atcagaccgc ctctcggtga acccccatgg gaagataacc ggggatgggc gcccccgcgg
     3721 cctggtcgat ggcctcctcg tcgaaccgct cgcggtcgtc ggagagacgc cagctccggg
     3781 cctccatgac tttcttgttc tgcaccgaca cgatcaggta cgagtagccg ggccaggcgt
     3841 gctcgcggtc gaactcgctg ggccgggccg ggtggtccgg gtgcgagtgg aagtatccca
     3901 cgatatcgag gcccagggcc cgcgcctcct tctgggcggc cagcacggtt tccgggctga
     3961 tcaggtaccg gttgtgccgg ctctcctggc gctcgttctg gaccgtgagg atccgctcga
     4021 cgatggtgct gtcgccctgc ggacggccga cgagcacgcc gcagcactcc tccggatagg
     4081 tcatggaggc gatccgcccg atggtgtgca gatgacgggg cgtgatgacg agacggttca
     4141 tgccgggatt tcctcccaga aacgttcgga cagagacttg tccgcgttgt cacacacgtg
     4201 gtgacgagcg tgcctccctg caaaccggaa gccattcccg gagctccggc cccagggggg
     4261 cggccacgat catgacctac acgctaacta aatcagtgtc agtttggcag gccggcggga
     4321 cagcctctgt caacgcaggg ggacgacgag ggaggcgagg atggagaggg cggtggcggc
     4381 caggcagccg atggccagga tcgtatggcc ggggcgctcc cccttccgcg ggtccaggac
     4441 cagggcggtc aggtagccgc cgacgaagcc gccgaggtgg gcccagttgt ccactcccct
     4501 gaagatgaag ccgaaggcga agaggatgat ggcgtaggtc caagcctgct ggccgatggc
     4561 gctgctgccg gcgcggcggc cataacagac cagggcgccg aggaggccga agatgggagc
     4621 cgaggcgccg agggtgatcg gagtcatgag cagggtgctg gccaggaagc cgcagaccga
     4681 ggagacggtg tagaggatga ccatgcggct ggcgccgtag agctccgcgg tctgcggggc
     4741 gagctggcgc acccacatga cgttgaagag gatgtggagg atcccggcgt ggagccagcc
     4801 ggcggacagg agggtccacc accggccgtg gcccaggacg gggatggcgc cgctggcgcc
     4861 gaagcgcagc atcgcctcct ggctcggggc gaggagcgac gccggcccca tgccgccgcc
     4921 caggccgaag cccggggtga cgacgagcat caggaggtag aggaggacgg agccgccgat
     4981 gaccgctttc tcgaaaccca ggtcctgccg gccgagcttg ccgagcacgg aggtgagccc
     5041 ccacatcccg gggttgcgcc ggccgcagtt gaggcagacc tcgtcgttga cccctacgag
     5101 gcggccgcag gaggggcaga ggatcgagcc ggagcgctgg gggcggcgga acatggtccg
     5161 gcgtactcta ccgcaggagg aggctcctca cacctgcatc ggcggctccc cgggatccgg
     5221 atcgcgccgc tcgggctcgc cgacgcccgg agcgtccagc ggatcctcga tcggctcgtc
     5281 ctggagcggc tcgtccgggt cctcctgcgg ctcctcgacg ttgggagggt ccaccggctc
     5341 ccgcgggtcg cggtccggcc gctcgtcgta cgggtccagg ggcggatcga tggcaggcgt
     5401 ctggtcgggc cggtcaattt ccatggtcgt cctcctcgct ccgcggcagt ccgcaagaat
     5461 cgggcccggg cagccttctt gctttcctcc ccgtccctgg aggtgaagca tggccccccc
     5521 cgacagctcc gagtggttgc tccaactttt ctggctcctg atcctggcgg tacccatcgc
     5581 ctgtgtggcg tggacggtga cgcacgagga gatcttccgg gagccgcggg agtactgcgt
     5641 cgagaaatcc aagaacgcgc cgagccttct ccaacgcaag ttcttctatc tgttcacctg
     5701 cgagtactgc ttcagccact acgtcacggt cttcttcctc ttcgtcaccc gctacaagct
     5761 gttgctggac gactggcggg gttatctcct ctcgttcttc gccctggtgg cggtggccaa
     5821 cgtctacatg agcgccttcg gccgtctgcg tcaggaggtc aagagcgaga agctggaggc
     5881 caagcgcaag gagaaggtgc tggaaagggt ggagcggcag gcggagcagg agaaggagga
     5941 gcaggagcag gagcaggaga acggccgggt cgtgcctgtc cccgtgggac agcggaggtg
     6001 aggacggctc aatccgccag cgactcgtcc agatcgtcct ccagcaggcc cggacgatcg
     6061 aggatctcgc gggcgcggcc ggcgtcggat tcgttgacgg cgagacggtt cagggccagg
     6121 atctcgccgg tgaacaggtt caccggcggc aggcgctcct ccagcacgca ggcctcgatc
     6181 ccctcggact cgagacgggt gcgcgccaga tgggcctccc agggcgtggt gaagcgggcg
     6241 acggtgacca agtctccaat cctctcctcg ccgtcctcca tctcctccgc ctcctccggc
     6301 tcatccatgg cgccggtgtc ctccggtttg accaggtaga tctccggaat gggacgcagc
     6361 tcccggagca gggcggacgc gcggtcggcc tcctcccggc gcacccggag cttgacgccg
     6421 ccgacgatgc cggcgccgta caaccggctc aggtgctcgt ccgcgatcac ggactcgacc
     6481 ccttcggcgt ccagacgcgc ccgggccagc tccgcctccc aggggtaggc gaaggtcgcc
     6541 acggtcacga gagaatccgg ttcgtacctc aggcccagca ttgtcgcacg gctacaagca
     6601 aaaggcgcgc cgggccccgg aggagcacgg gctcaagcgt gcccgccgct gcggagcaac
     6661 aggtacagcc cgagggtgag gccggtgtag aagaggaaga tgatgaccag cgcccacacc
     6721 ccggcggcgc ggccccgggg gtgatggtgg gcggcggcga gatagccgcc caccatcgag
     6781 agcacgatga agaggacgta gatcgtcagc ggaacggcca tgccggtgcc ggggtctcag
     6841 tccttgtaga aggtctcgcc cttctccgcc atctgccgca gcagcggcgc cggctccagg
     6901 cgctggccgt gaatggcggc gtaggcgtcc agccggtcca ccacgtagcg caggcccagc
     6961 cggtcggcct cgtgcaggag gccaccccgg aacggcggga agccgaagcc gaagatgacg
     7021 ccgatgtcca cgtcggcggg gttggtgatg atcccctcct ccatgcagcg ggcggtctcg
     7081 ttgagcatct gcatccagca gcgctccacc acctcccgcc cgtccaccgg cgattcccgc
     7141 cagtcgagga gcctgtagac ggcctcgtcc accttcttgg ccttcctgga gccttcgccg
     7201 cccttcttct ttttcccgtc ctcgtcgccg tagaggtaga agcccttctt cgccttccgg
     7261 ccgagccggc cgtcgtcgat catgcgcgcg aagacggcgg ggggcgagaa gcgctccccc
     7321 ttgtaggcca gcaggatctc gcccacgtgc ttgccgatgt cgaggcccac ctcgtccatc
     7381 agctcgaacg gccccacggg ccagccccac tcccgcatca ccttgtccac cttgtcgacg
     7441 cgggcgccct ccaccagcag ccaggccgcc tcgttgagga acggcgccag cacacgggtg
     7501 gtgaagaagc cggggccgtc gttgaccacg atgacggtct tgcccatggt gcggccgatc
     7561 tcgaccgccg tgcccagggc ctcgtccgag gtgccggggt ggcggacgac ctccagcagc
     7621 ggcatcttgg ccaccggact gaagaagtgc atgcccacca cgttctccgg ccggctgctc
     7681 ccctcggcca gacgggcgat ggggatcgtc gaggtgttgg aggcgaagat caggttgtcc
     7741 ggggccacgg cctcggtctc gcggatgacc gtgtgcttga tccccacgtc ctcgaacacc
     7801 gcctcgacga cgaagtcgac gccgcggaaa tcgtggtagt cgaccgtcgg gatgatgcgg
     7861 gccatggcgc gccgcgcctc ggcgtcggac aggcggcggc gcttcttgag ctcgttgagc
     7921 tgctcgccgc agtgcttcat cccccggccg agggcgtcga ggtccttgtc cttgaggacg
     7981 atgggcatgt ccttgtaggc cagcacctgg gcgatgccgg aacccatgaa gccggcgccc
     8041 aagatgccca ccacgtccac cggccgcccc tgtctggcga tgcggccggc ccgcgcctcc
     8101 acctcgttct tcatgaagaa gatggacatg aggctcttgg cggtgtcgga gagcaccagc
     8161 tcggagaagg cgccggcctc gacgtccagg gcgcggcgca gggggagctt gaggccgtcg
     8221 cgcaccacgg agatggcgac cagcggcgcc gggtagtggc cgccggtctt ttccatgacg
     8281 ccggcgcgtg ccttctccca gacgaagcgg tccgccacca ggggggtgtg ggccaggagg
     8341 tctccggcgc gcatggggag cgaccggccc gccttgcgct tcttctcgat ccccttgagc
     8401 tccttccccc ccttgcggcc gaggcccacc cagcgctggg cggcctcccg caggtcggag
     8461 gggtggcagg tgtcgtccac gaggccgagg cggcgggcct tcttggcgtc gacctgcttg
     8521 ccggtgagga tgaggtcgag agcgtcgggc acgccgatga gccggggcag ccgctgggtg
     8581 ccgccgagtc ccgggatgag gccgagctgg acctcgggga ggccga
//
LOCUS       DASPJI010000372         3646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_81370, whole genome shotgun sequence.
ACCESSION   DASPJI010000372 DASPJI010000000
VERSION     DASPJI010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81370"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /locus_tag="VEL74_10000"
     CDS             complement(<1..1455)
                     /locus_tag="VEL74_10000"
                     /inference="COORDINATES: protein motif:HMM:NF023855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HYG62902.1"
                     /translation="MSIHIDREIARDLSRSAGMEWLETNGLGGWAGTTVAGLHSRRCH
                     GLLVAATEPPGGRAVLLSRLDETLHVAGETFELSCNQFPGAIAPRGFEHLASFSKDLF
                     PVFEYEAGGIRLRKTVAAVDGENTTLVLYEVLEAPGPFLLSLRPFVAGRHQHALAHAN
                     PALSEETVFEDSVLRLHPYADLPEVFLQVPGAELRANPHWWYRFEYELDRRRGSDFQE
                     DLWTPGLFGRELGPGDRLGIVLSTRDPRGRDAFELLDRERRRREKVVRAVPVRDETAR
                     ALTLAADGFLVRRGSVQGEQKGQRAVVAAYPAPGERSRDTMIALPGLCLATGRYEEAK
                     KILRTFARGLEGGLFPGDTSVDASLWFFVAGYRFFKATDDEAFTRGTLLPALRKVIHA
                     YDRGTLHDIKADEDGLLLANEPEIPLTWMDAVVAGRPVTSRAGKAVEVQALWHNALCI
                     LAELEARLGDPAEANWLEKRARRVQKRFADLFWDE"
     gene            complement(1627..>3646)
                     /gene="parC"
                     /locus_tag="VEL74_10005"
     CDS             complement(1627..>3646)
                     /gene="parC"
                     /locus_tag="VEL74_10005"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003285192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HYG62903.1"
                     /translation="DVLGKYHPHGDTACYEAMVLMAQPFSLRYPLIDGQGNWGSQDDP
                     KSFAAMRYTEARMTPFSQILLSELEQGTVEWGPNFDGTLQEPKLMPARLPHVLLNGAS
                     GIAVGMATDIPPHNVGEITAACLRLLEEPNAPDDEIFASILGPDFPTDAEVITPAAEI
                     RKIYETGSGSLRMRATFEQEDGEVIMTALPYQVSGSKVLTQIAAQMQAKKLPMVEDLR
                     DESDHENPTRLVIVPRSNRVDVEALMAHLFATTDLERSYRVNLTMIGLDGRPRQFNLR
                     GLLEEWLRFRIETVRRRLRFRYDKVLARLHILEGFLAAYLNLDEVIRIIRTEDEPKPV
                     LMDRFTLTDIQAEAILELKLRHLARLEEMKIRGEQTSLMEERDALEKTLGSETRLRRK
                     VRDELAEDAKTFGDARRSPIVERGAARALDESELMPTEPVTVVLSERGWVRAAKGHEV
                     DPKELSYKAGDAYLQSVKGRSNQFAVFFDSTGRSYSLPAHGLPSARGQGEPLSSHLTP
                     PSGASFVSVMMGSDEDLYILATTAGYGFLVKLGDLQSRNRAGKAVLNVPAGAKVLPPQ
                     KVNNLQTDRIAAATDSGRLLIFPLQDLPLLPRGKGVKILNIPGGKGEPEALAAAIALP
                     PGAHLMVYAGKRHLNMRPADYETYIGSRALRGNKLPRGFQNVAGLAVE"
BASE COUNT          519 a         1210 c         1359 g          558 t
ORIGIN      
        1 ctcgtcccag aacagatcgg cgaagcgctt ctggacccgg cgcgcccgct tctccagcca
       61 gttggcctcg gccggatcgc ccagtcgggc ctcgagctcg gcgaggatgc agagggcgtt
      121 gtgccagagg gcctggacct ccaccgcctt gcccgcccgg gacgtcaccg gccggccggc
      181 caccaccgcg tccatccagg tgagggggat ctcgggctcg ttcgctagca ggaggccgtc
      241 ttcgtcggcc ttgatgtcgt ggagggtgcc gcggtcgtag gcgtggatca ccttgcgcag
      301 cgccggcagg agcgtgccgc gggtaaaggc ctcgtcatcg gtggccttga agaagcggta
      361 gccggcgacg aagaaccaga gggaggcgtc gacggaggtg tcgccgggaa agagcccgcc
      421 ttcgaggccg cgggcgaagg tgcggaggat cttcttcgcc tcctcgtagc ggccggtggc
      481 cagacagagg ccgggcaggg cgatcatcgt gtcgcggctc cgctcgccgg gcgccgggta
      541 ggcggcgacc actgcccgct gccccttctg ctccccctga acgctgccgc ggcgcaccag
      601 gaagccgtcg gctgccaggg tcagcgcgcg ggcggtctcg tcccggacgg ggacggcccg
      661 cacgaccttc tcgcgccgcc gccgctcccg gtcgagcagc tcgaaggcgt cgcgcccgcg
      721 cgggtctcgg gtggagagga cgatccccag gcggtcgccc ggcccgagct cgcggccgaa
      781 gaggccgggg gtccagaggt cttcctggaa atcgctgccg cgccggcggt cgagctcgta
      841 ctcgaaccgg taccaccagt gcgggttggc gcggagctcg gcgcccggaa cctggaggaa
      901 gacctcgggg aggtcggcgt aggggtggag ccgcagcacg ctgtcctcga agaccgtctc
      961 ctcggagagg gcggggttgg catgggccag ggcgtgctgg tgccggccgg cgacgaacgg
     1021 gcgtagcgac aggaggaagg ggccgggtgc ctccagcacc tcgtagagca ccagggtggt
     1081 gttctcgccg tcgacggcgg ccacggtctt gcgcaggcgg atgccgccgg cctcgtactc
     1141 gaagacgggg aagaggtcct tactgaagga ggccagatgc tcgaacccgc gcggagcgat
     1201 ggcgccgggg aactggttgc agctcagctc gaaggtctca cccgccacgt gcagggtctc
     1261 gtcgagacgg gacagcagga cggcccggcc accgggcggt tcggtagcgg cgacgagcag
     1321 gccgtggcag cggcggctat gaagcccggc gacggtggtg ccggcccagc cgccgagacc
     1381 gttggtctcg agccactcca tgccggccga gcgcgagagg tcccgggcga tctcccggtc
     1441 gatatggatg gacatctaca tctccttcgg ccggacggcg ccggctatga ctccggaccc
     1501 cggagggtcg aaccatgagg gagccccgtg gaggtccgcg gggcacggcg gggccggatc
     1561 gctcctgccg gccggagttt agcaggatga aaaatcagga agggagggcg aggggcggcg
     1621 gagggcctac tccaccgcca gcccggccac gttctggaag ccgcgcggca gcttgttgcc
     1681 gcgcagagcc cgggagccga tgtaggtctc gtagtcggcg ggacgcatgt tgaggtgacg
     1741 cttgccggcg taaaccatga ggtgggcccc cggcggcagc gcgatggcgg cggccagcgc
     1801 ctcgggctcc cccttccctc ccgggatgtt gaggatcttc acccccttgc cgcgcgggag
     1861 cagcggcagg tcctgcaacg ggaagatcag gagacgcccg gagtcggtgg cggcggcgat
     1921 ccggtcggtc tgcaggttgt tcaccttctg cggcggcagg accttggcgc cagcggggac
     1981 gttcagcacc gccttacctg cccggttgcg gctctggagg tctcccagct tcaccaggaa
     2041 gccgtagccg gcggtggtcg ccaggatata gaggtcctcg tcgctgccca tcatcaccga
     2101 cacgaagctg gcgcccgacg ggggcgtcag gtggctcgac agcggctcgc cctggccccg
     2161 ggccgagggc aggccgtgcg ccggcagcga gtagctgcgg ccggtcgagt cgaagaagac
     2221 ggcaaattga ttgcttctcc ccttgaccga ctggaggtag gcgtcgccgg ccttgtagga
     2281 cagctccttg ggatccacct cgtgtccctt ggccgcgcgc acccagccgc gctcggagag
     2341 caccacggtc accggctcgg tgggcatcag ctcggactcg tccagcgccc gggcggcgcc
     2401 gcgctcgacg atcggcgagc ggcgggcgtc gccgaaggtc ttcgcgtcct cggccagctc
     2461 gtcgcggacc ttgcggcgca ggcgcgtctc ggagccgagc gtcttctcca gggcgtcccg
     2521 ctcctccatc aggctcgtct gctcgccccg gatcttcatc tcctcgagac gggccaggtg
     2581 gcgcagcttg agctcgagga tcgcctcggc ctggatgtcc gtcagggtga agcggtccat
     2641 cagcaccggc ttgggctcgt cctcggtgcg gatgatccgg atcacctcgt cgaggttgag
     2701 gtaggcggcg aggaacccct ccaggatgtg gagccgcgcc agcaccttgt cgtagcggaa
     2761 gcgcaggcgc cgccgcaccg tctcgatgcg gaagcgcagc cactcctcga gcagtccgcg
     2821 caggttgaac tgccgcggcc ggccgtcgag gccgatcatg gtcaggttca cccggtagct
     2881 ccgctcgagg tcggtggtgg cgaagaggtg ggccatcagc gcctcgacgt ccacccggtt
     2941 cgagcgcggc acgatcacca ggcgggtcgg gttctcgtgg tccgactcgt cccgcaggtc
     3001 ctccaccatg gggagcttct tggcctgcat ctgggcggcg atctgggtca gcaccttgct
     3061 gcctgagacc tggtagggca gggcggtcat gatgacctcg ccgtcctcct gctcgaaggt
     3121 ggcccgcatg cgcagcgacc ccgagccggt ctcgtagatc ttgcggatct cggccgccgg
     3181 cgtgatcacc tcggcgtcgg tcgggaagtc gggccccagg atggacgcga agatctcgtc
     3241 gtccggcgcg ttcggctcct cgaggaggcg caggcaggcc gcggtgatct cgccgacgtt
     3301 gtgcggcggg atgtcggtcg ccatgccgac ggcgatgccc gaggcgccgt tgagcagaac
     3361 gtgcggcagg cgcgccggca tcagcttcgg ctcctggagg gtgccgtcga agttgggccc
     3421 ccattcgacc gtcccctgct ccagctccga cagcaggatc tgcgagaacg gcgtcatgcg
     3481 cgcctcggtg tagcgcatgg cggcgaacga cttgggatcg tcctgggagc cccagttacc
     3541 ctggccgtcg atgagcgggt agcgcaggct gaacggctgc gccatgagga ccatcgcctc
     3601 gtagcaggcg gtgtcgccgt gcgggtggta cttgcccagc acgtcg
//
LOCUS       DASPJI010000373         3311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_137691, whole genome shotgun sequence.
ACCESSION   DASPJI010000373 DASPJI010000000
VERSION     DASPJI010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137691"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VEL74_10010"
     CDS             complement(<1..265)
                     /locus_tag="VEL74_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62904.1"
                     /translation="MPEHAPEIWRTACLQMAWRARGGVVVYPALVGFLGLGSDLSRRA
                     PTLFAALLASVTLIAAVRQILIRRFDPIFDRWPRLWETGFFGGL"
     gene            464..1654
                     /locus_tag="VEL74_10015"
     CDS             464..1654
                     /locus_tag="VEL74_10015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HYG62905.1"
                     /translation="MAFPDQPPSAPSHVAPPLAARPAWAVPRVWPWLIGSFVVIGLVE
                     VVRGYTLAYLGGEPQTWWHVFVSTFPFWGCLGFLLAPLVPVIHRFPLELRRWRRSLAI
                     HLAIGLPFGVLCAVVAPLIADLLHRGYLQPPIRYFLRLFHWTAVPGFYSYAALVAILS
                     ALDSHRRYVEEERAAARLAVRASELEASLAQSRLETLRMQLNPHFLFNTLNATSVLAL
                     KGERDKVAAMLTRLSDLLRAVLESSAQVVPLADELALLERYLEIERVRFGDRLTVVVE
                     ADPAVLEAEVPGLVLQPLAENAVRHGISRQPGPGRLEVRAKAGGGRMVIEVRDSGPGF
                     GGAEGGARGLGLANTRERLAQLYGGAAVLELLDAPGGGGLVRLELPLRAYVAGGTVGE
                     GHHA"
     gene            1647..2417
                     /locus_tag="VEL74_10020"
     CDS             1647..2417
                     /locus_tag="VEL74_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HYG62906.1"
                     /translation="MPETIRTLIVDDEPLAREGVRLHLAGEPDIEVVGECGDGEAAVE
                     AIRDLAPDLLFLDIQLPGLDGFEVLDRVPERVPAVVFVTAYDRHAVAAFERHALDYLL
                     KPYDAPRFARALARVRDELARRDREAVGREVLEVLRELRGRPGYLQRVAVRTAGRYVF
                     LRLDEIDWIEAAENYVRLHAGGRGHLLRETMAGLEGRLDPERFLRVHRSAIVRVDAIQ
                     ALERLSNNEFAVVLADGTRLVSSRGYRERIEDLLRRSR"
     gene            2499..>3311
                     /locus_tag="VEL74_10025"
     CDS             2499..>3311
                     /locus_tag="VEL74_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62907.1"
                     /translation="MDRRNSQDNRQDRGAGLPWIEVSPGVPYFQTEVGESWTPIGQND
                     AITWPDLEGLFRRRDLPGTERYLAGLAASGVTCVRLMLEYAQGEHRYLERPPGTFWPA
                     MVQLWDDLFALCARHRIRVLLTPFDTFWMWRRWKHHPYNRKNGGVCAARKRFLSCPDT
                     RAAIRRRLLFATERWGGTGTLFAWDLWNEIHPAYCGDDTAPFTELIEDLSGTVRRREL
                     ELYGRSHPQTVSIFGPLIKERPEVAESVFRHPRLDFASTHLYETGTIDFPRNT"
BASE COUNT          424 a         1227 c         1166 g          494 t
ORIGIN      
        1 gcaggccgcc gaagaagccg gtctcccaga gccggggcca gcggtcgaag atcgggtcga
       61 agcggcggat caggatctgc cggacggcgg cgatcagggt gacggacgcc aggagggccg
      121 cgaagagggt gggggcccgc cgtgacaggt cggagccgag gccgaggaag ccgaccagcg
      181 ccgggtagac gaccacgccg ccgcgggcgc gccaagccat ctggaggcag gccgtccgcc
      241 agatttcggg cgcatgctcg ggcaccgtct cccgcaaacc ggaacctcga ttcgatgcaa
      301 ttcagaacga cagggcttgg tgacagccta gtcgaggtcg tccgggcggt caagccgagc
      361 ccgtccccgg atcccgccgg atcgtccctg gcggcaggcg acccgtcccg gcgcccgggg
      421 ggagccctgg cggcgcgggc gcccctgccg tacccttccg cggatggcgt tcccggacca
      481 gcctccctcc gccccctccc acgtggcgcc gccgctcgcg gcgcggccgg cctgggcggt
      541 gccccgggtc tggccctggc tcatcggctc cttcgtcgtg atcgggctcg tcgaggtggt
      601 gcgcggctac accctggcct atctcggagg cgagcctcag acttggtggc acgtcttcgt
      661 ctccaccttc cccttctggg ggtgcctggg cttcctgctg gcgcccctcg tcccggtgat
      721 ccaccgcttc ccgctggagc tgcggcgatg gcgccgctcg ctggccatcc acctggcgat
      781 cgggctccct ttcggcgtcc tctgtgccgt cgtcgctccc ctgatcgccg accttctcca
      841 tcgcggatat ctccagcccc cgatccgcta cttcctgcgc ctgttccatt ggacggcggt
      901 gccgggcttc tactcctacg cggccctggt ggcgatcctc tccgccctcg actcccatcg
      961 ccggtacgtc gaggaggagc gcgccgccgc ccgcctcgcg gtgcgcgcct cggagctgga
     1021 ggcgagcctg gcccagtccc ggctcgagac cctgcgcatg cagctcaacc cgcactttct
     1081 gttcaacact ctgaacgcca cctcggtgct cgccctcaag ggggagcgcg acaaggtggc
     1141 ggccatgctc acccggctga gcgacctgct gcgcgccgtg ctcgagagct ccgcccaggt
     1201 ggtgcccctg gcggacgagc tcgccctgct ggagcgctac ctggagatcg agcgggtgcg
     1261 gttcggggac cgcctgaccg tggtggtgga ggcggacccc gcggtcctgg aggcggaggt
     1321 gcccggcctg gtgctccagc cgctggccga gaacgccgtc cgccacggca tctcgcgcca
     1381 gcccggcccg gggcgcctgg aggtccgggc gaaggccggc ggcgggagga tggtcatcga
     1441 ggtgcgcgac agcgggcccg gcttcggcgg cgccgagggt ggggcacggg ggttggggct
     1501 cgccaacacc cgcgagcggc tggcgcagct ctacggcggc gcggcggtcc tggagctgct
     1561 cgacgcgccc ggggggggcg gcctggtccg gttggagctg ccgctgcggg cctacgttgc
     1621 cgggggtacg gtgggggagg gacaccatgc ctgagacgat ccggaccctg atcgtcgacg
     1681 acgagccgct ggcgcgcgaa ggcgtccggc tccacctggc cggcgagccg gacatcgagg
     1741 tggtgggcga gtgcggcgac ggcgaggccg ccgtcgaggc gatccgcgac ctggcgccgg
     1801 acctcctctt cctcgacatc cagctccccg gcctggacgg cttcgaggtg ctcgaccgcg
     1861 tgccggagcg agtgccggcg gtggtcttcg tcaccgccta cgaccgccac gccgtggccg
     1921 ccttcgagcg ccacgccctg gactatctgc tcaagcccta cgacgcgccg cgcttcgccc
     1981 gcgccctggc ccgggtccgc gacgagctcg cccggcgcga ccgcgaggcg gtcggccgcg
     2041 aggtgctgga ggtgctgcgc gagctacgcg gccgccccgg ctatctgcaa agggtcgcgg
     2101 tgcgcacggc cgggcgctac gtcttcctgc gcctggacga gatcgactgg atcgaggcgg
     2161 ccgagaacta cgtgcgcctc cacgccggcg ggcgcggcca cctgctgcgc gagaccatgg
     2221 ccggcctcga aggacggctc gacccggaac gcttcctgcg cgtccaccgc tcggccatcg
     2281 tgcgcgtcga cgccatccag gcgctggaaa ggttgtcgaa caacgagttc gccgtcgtcc
     2341 tcgccgacgg cacccgtctg gtgtcgagcc gcggctaccg cgagcggatc gaggacctgc
     2401 tccgccgctc gcgctgaatc cccggtggct ctggcctgcc gtttgcaact ctgcgcctct
     2461 cctgcggccc catttgttcg gaggccgtgc cgtacgggat ggatcgacgc aactcacagg
     2521 acaaccggca ggatcggggg gcggggctcc cctggatcga ggtatctccg ggagtgccct
     2581 acttccagac ggaggtgggg gagagctgga cgcccatcgg ccagaacgac gccatcacct
     2641 ggccggacct cgaggggctc ttccggcgcc gcgacctgcc gggaaccgag cgctacctcg
     2701 ccggcctcgc cgcgagcggg gtcacctgcg tccggctgat gctcgagtac gcccaggggg
     2761 agcaccgtta cctcgagcgg ccgcccggca ccttctggcc ggccatggtc cagctctggg
     2821 acgacctctt cgccctctgc gcccggcacc gcatccgggt cctcctcacg ccgttcgaca
     2881 ccttctggat gtggcggcgc tggaagcacc acccctacaa ccggaagaac ggcggcgtct
     2941 gcgccgcccg caagcggttc ctctcctgtc ccgacacacg cgccgcgatc cggcgccggc
     3001 tcctcttcgc caccgagcgc tggggcggca ccggtaccct gttcgcctgg gatctctgga
     3061 acgagatcca ccccgcctat tgcggcgacg acacggcgcc gttcaccgag ctgatcgaag
     3121 acctgagcgg gacggtccgc cgccgcgagc tggagcttta cgggcggtcc catccgcaga
     3181 ccgtctccat cttcgggccg ctgatcaaag agcggcccga ggtggccgag agcgtcttcc
     3241 ggcatccgcg gctcgacttc gccagcaccc acctgtacga gacgggcacc atcgatttcc
     3301 cccggaacac g
//
LOCUS       DASPJI010000374         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_766965, whole genome shotgun sequence.
ACCESSION   DASPJI010000374 DASPJI010000000
VERSION     DASPJI010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766965"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VEL74_10030"
     CDS             complement(<1..667)
                     /locus_tag="VEL74_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HYG62908.1"
                     /translation="MKRVNLAWILALLAGLVACGPSEEGAADREADRAPAVEAVPART
                     GTLPLEERLNGVVRADNQITIQSEIEAPILEVLGRNGQAVERGQPLVRLDGDELRQQL
                     RQAEADVRLAEATAREARARVAEVEALVTRTRRLAQEELVSALDLETQEARLAATRAS
                     ADQARARVEQARAVVQERRSALDKTLIRAPVAGHLGQRNAEVGMLADASDVLFVLGNL
                     DDLV"
     gene            782..>2585
                     /locus_tag="VEL74_10035"
     CDS             782..>2585
                     /locus_tag="VEL74_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYG62909.1"
                     /translation="MKVAVLGGGPAGLYFALLMKKADPRHQVTVVERNPPDATFGWGV
                     VFSDQTLGNFKAADPESFQEILDNFARWDDIDIHFKGKTITSGGHGFAGIARVKLLQI
                     LQERAAGLGADLRFGVEVRDDSELPGLGLGDADLIVAADGVNSAIRRRYAGEFRPDVI
                     SGKAKFIWLGTTRRFDAFTFVIVENQAGVFQLHAYQFSDDRSAFIVECDELSWRNAGF
                     DRMDIDATIDACEQMFEPWLQGHRLLANIPPHQRAAPWLNFQRVHCARWWHDNVVLIG
                     DAAHTAHFSIGSGTKLAMEDAIGLSRILNEGRPLPESLQAYEEERRIEALRLQNAARN
                     SMEWFENVRRYARLDPEQFAYSLLTRSQRVSHENLRLRDRVYLEGVERWFASRASHVR
                     RQELPKAPPPMLTPFTLRGMTVDNRVVVSPMDMYSAVDGTPNDFHLVHLGSRALGGAG
                     LVVTEMTCVSPEGRITLGCTGLYRPEHLEAWRRVTGFVHQWSRSKICLQLGHSGGKGS
                     TRLMWEGMDEPLAEGAWPVIGPSPIPYKPGMQVPREMTRADMDKVRDDFVRATRMAID
                     AGFDMVELHCAHGYLLSSFITPLTNKRTDEYGGSL"
BASE COUNT          376 a          938 c          878 g          393 t
ORIGIN      
        1 ccacgaggtc gtccaggttg ccgaggacga agaggacgtc ggaggcatcg gccagcatcc
       61 ccacttcggc gttccgctgc ccgaggtggc cggccaccgg cgcccggatg agggtcttgt
      121 cgagcgccga ccgccgctcc tggacgaccg cgcgggcctg ctcgacgcgg gcccgcgcct
      181 ggtcggcgct cgcccgggtg gcggcgagcc gggcctcctg ggtctccagg tccagcgcgc
      241 tcaccagctc ctcctgggcg agccggcggg tgcgggtcac cagggcctcg acctcggcca
      301 cgcgggcccg ggcctcccgg gcggtggcct cggccagccg cacgtccgcc tcggcctgcc
      361 ggagctgctg ccgcagctcg tcgccgtcca ggcgcaccag gggctgtccg cgctcgacgg
      421 cctggccgtt gcgtcccagg acctcgagga tcggggcctc gatctcggac tggatggtga
      481 tctggttgtc ggcccggacg acgccgttca gccgttcctc cagggggagg gtgccggtgc
      541 gggccgggac ggcctcgacc gccggcgcgc ggtccgcttc ccggtccgcg gcgccctcct
      601 ccgaggggcc gcaggcgaca aggcccgcca gaagggccag gatccatgcc aggttgactc
      661 gtttcatcgt gtttacaagg ctcacacttc ccccccgggc cggggacttg cagattccaa
      721 gccgtgctag catccggccg gtgatgccca ccgtcctccc cccatgctcc cggaggttcc
      781 tatgaaggtc gccgtcctcg gtggcgggcc ggcggggctg tacttcgccc tgctgatgaa
      841 gaaggcggac ccgcggcacc aggtcacggt ggtggagcgc aatccgcccg acgccacatt
      901 cgggtggggc gtcgtgttct ccgaccagac gctcggcaac ttcaaggcgg ccgatcccga
      961 gtcgttccag gagatcctgg acaacttcgc ccggtgggac gacatcgaca tccacttcaa
     1021 ggggaagacc atcacctccg gcggccatgg gttcgccggc atcgcccggg tcaagctgct
     1081 gcagatcctc caggagcgcg ccgccgggct gggcgccgac ctccgcttcg gcgtcgaggt
     1141 gcgggacgac tccgagctgc cggggctggg ccttggggac gcggacctga tcgtcgcggc
     1201 ggacggggtc aacagcgcca tccgcaggag gtacgcgggg gaattccggc cggacgtcat
     1261 ctccggcaag gccaagttca tctggctcgg caccacgcgc cggttcgacg ccttcacctt
     1321 cgtcatcgtc gagaaccagg ccggggtctt ccagctccat gcgtatcagt tcagcgacga
     1381 ccggtcggcg ttcatcgtcg agtgcgacga gctgtcctgg cgcaatgccg gcttcgaccg
     1441 gatggacatc gacgccacca tcgatgcctg cgagcagatg ttcgagccct ggctccaggg
     1501 gcaccggctg ctggccaaca tcccacccca ccagcgcgcg gcaccgtggc tcaacttcca
     1561 gcgcgtccac tgcgcccgct ggtggcacga caatgtcgtc ctcatcggcg acgcggccca
     1621 caccgctcat ttctccatcg gctcgggcac caagctcgcc atggaggacg ccatcggcct
     1681 gtcgcgcatc ctcaacgagg ggcggccgct ccccgagtcc ctccaggcct atgaagagga
     1741 gcggcgcatc gaggcactgc gtctccagaa cgccgcccgc aactccatgg agtggttcga
     1801 gaacgtccgg cgctacgcgc ggctcgatcc cgagcagttc gcctacagcc tgctcacccg
     1861 cagccagcgg gtcagccacg agaacctccg gctgcgcgac cgcgtctacc tcgaaggggt
     1921 cgagcgctgg ttcgccagcc gtgccagcca cgtccgcaga caggagctgc ccaaggcgcc
     1981 gccgccgatg ctcacgccgt tcaccctgcg cggcatgacg gtcgacaacc gggtggtggt
     2041 ctcgcccatg gacatgtaca gcgccgtgga cggcacgccc aacgacttcc atctcgtcca
     2101 cctgggctcc cgggcgctgg gcggcgccgg gctggtggtc accgagatga cctgcgtctc
     2161 tcccgagggg cggatcacgc tggggtgcac cggtctgtac cggcccgagc acctggaggc
     2221 ctggcgccgg gtcaccgggt tcgtccacca gtggagccgt tcgaagatct gcctgcaact
     2281 cggccactcc ggcggaaagg ggtccacccg gctcatgtgg gaggggatgg acgagccgct
     2341 cgcggagggc gcgtggccgg tcatcgggcc gtcgcccatc ccctacaagc cgggcatgca
     2401 ggtgccgcgg gagatgaccc gcgcggacat ggacaaggtg cgggacgact tcgtccgcgc
     2461 cacccggatg gcgatcgacg ccgggttcga catggtcgag ctccactgcg cccacggcta
     2521 tctgctttcg agcttcatca cgcctctgac caacaaacgc accgacgaat atggcggcag
     2581 cctcg
//
LOCUS       DASPJI010000375         3738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_318370, whole genome shotgun sequence.
ACCESSION   DASPJI010000375 DASPJI010000000
VERSION     DASPJI010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318370"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3738
                     /locus_tag="VEL74_10040"
     CDS             <1..>3738
                     /locus_tag="VEL74_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62910.1"
                     /translation="DAFGVELPLRVLFEAPVLSDLAARVDAARQGASPAPPIVRSAGE
                     LPPLSFAQERLWFLDRLEPGSAAYNLATALRLSGDLDVPALAASLAEIARRHAALRTT
                     FAEVAGEPRQIIAPPSDLPLPLVDLAALPGAEREAEATRLVHEDAVRPFDLARGPLVR
                     ALLLRFESREHGLILDTHHIVSDGWSQGVLVRESASLYEAFLHRRPSPLPELGIQYAD
                     FAVWQRRWLTGEVLEAQLAYWRETLESAPALLELPLDRPRPPVQTFRGAVEPVALTEE
                     LTAALRELGRREGVTLFMTLLAGFQALLHRLSGQDDVLVGSPVANRNRSETEGLIGFF
                     VNTLVLRARFGSSQPEGLDVRTLLATARAAALEAYAHQDLPFERLVEELRIERSLAHG
                     PLFQVMLALQNAPVGRLELPGLLLAPMGTGGGTAKFYLTLSLGESEGPLAGSLEYNTD
                     LFDGSTVRRLGEQLEVLLAAAVADPDRRVRELPLLTTAQRSQLLVEWNDTARVRPLSP
                     LVHERVAAWAELRPNAEAVVSRSRKLTYGELAARANRLAWHLRSLGVGPEVRVAVCMG
                     WTVERVVAALAVLQAGGAYVTLDPTYPPERLAYLIADSRAPVVITEARYRDHLTETGA
                     TVLSLDADWESITGREDVPPESGAGPENLAYVVYTSGSTGEPKGVEVPHAGLLNLVLW
                     HQERYAVHPDDRAGQVASPAFDASVWDLWPYLAAGAASYIPDEETRLSPTAMVRWWME
                     EGITLTFLPTPLAEAVLDEAVPPESELALRALLVGGDRLHHAPGPEWPFRLVNHYGPA
                     ECSVVSTALDIPAEPWGHRGGVPPIGRGIDTARLYVLDTEGELVPPGVPGELYIGGAG
                     LARGYLGRPELTAAAFLPNPFAGEAGSRMYRTGDRVRWRTGGVLDFLGRFDHQVKVRG
                     IRIELGEIEAVLARHPAVREAAVVLLRGGRQGEVRLVACVTPAEEGRTSTDELRAFLR
                     ERLPEAMVPAAFLFVDALPLTPNGKVDRRALEALAPGAEAGTESLSAAPRTPTEEIIA
                     GIWADLLGRERVSTGDDFFALGGHSLLATRVVSRVRDAFGVELPLRRLFDAPTVEALA
                     AAIDAAVGAVRGEAGGLPVPPLTAGLRGDRPRLSFAQERLWFLDQLDPSAASYSVSLA
                     LRLHGGLDPEVFARILTEVVRRHEVLRTTFAHAGGQPYQVIGPPAAVPVPVVDLRGMS
                     GAERDEEVALRARAEAARPFDLANGPLLRALLLRTGEEDWVA"
BASE COUNT          435 a         1393 c         1364 g          546 t
ORIGIN      
        1 gacgccttcg gcgtggagct gcccctgcga gtgttgttcg aggccccggt gctgtccgat
       61 ctggcggcgc gggtcgacgc cgcgcgccag ggggcgagcc cggcgcctcc catcgtgcgc
      121 tcggcgggcg agctgccacc cctctccttc gcccaggagc ggctctggtt cctcgaccgc
      181 ctggagccgg gcagcgccgc ctacaacctc gccaccgcgt tgcgcctgag cggcgacctg
      241 gacgtgcccg cactggccgc gagcctggcc gagatcgccc gccgtcacgc ggcgctgcgc
      301 accaccttcg ccgaggtggc gggggagccg cggcagatca tcgctccccc ctcggacctg
      361 ccgctgccgc tggtggacct cgcggcgctg cccggtgcgg agcgggaggc cgaggcgacg
      421 cgcctggtcc acgaggacgc cgtgcgcccg ttcgacctcg cccgcgggcc gctggtccgt
      481 gccctgctgc tgcggttcga gagccgggag cacgggctga tcctcgacac gcatcacatc
      541 gtgagcgacg gctggtcgca gggcgtgctg gtgcgcgaga gcgcctccct gtacgaagcc
      601 ttcctccatc ggcggccgtc gccgctgccc gagctgggta tccaatatgc cgacttcgcc
      661 gtctggcagc gccggtggct caccggcgag gtgctggagg cccagctcgc ctactggcgc
      721 gagacgctgg agagtgcgcc ggctctcctg gagctgcccc tcgaccggcc gcggccgccg
      781 gtccagacgt tccgcggcgc cgtcgagccg gtggcgctca cggaggagct gaccgcagcc
      841 ctgcgtgagc tcggccggcg cgagggggtc accctgttca tgaccctgct cgccggcttc
      901 caggcgctgc tgcaccgcct ctccggccag gacgacgtgc tggtgggctc gcccgtggca
      961 aaccgcaacc gcagcgagac cgaggggctc atcggcttct tcgtcaacac cctggtgctg
     1021 cgggcgcggt tcggctcctc acagcccgaa ggcctggacg tccgtaccct cctggccacg
     1081 gcgcgcgccg cggcgctgga agcctacgcc caccaggacc tgccgttcga gcgtctggtg
     1141 gaggagctgc ggatcgaacg gagcctggcc cacgggcctc tgttccaggt catgctcgcc
     1201 ctgcagaacg cgccggtcgg acgtctggag ctgccgggcc tcctcctggc gcccatgggc
     1261 accggcggcg gcacggccaa gttctacctc acgctctctc tgggcgagag cgaagggccg
     1321 ctggcgggaa gcctggagta caacaccgac ctgttcgacg gctccaccgt gcgccgcctc
     1381 ggcgagcagc tcgaagtgct cctcgccgcg gcggtggccg atccggaccg gcgtgtccgg
     1441 gagctgccgc tgctcaccac ggcccagaga tcgcagctcc tggtggagtg gaacgacacc
     1501 gcccgggttc gcccgctgtc tcccctggtc cacgagcggg tggcggcctg ggccgagctg
     1561 cggcccaacg ccgaagccgt ggtctcccgg agccggaagc tcacctacgg tgagctcgcg
     1621 gcacgcgcca accgcctggc ctggcacctg cggtcgctgg gagtcggtcc cgaggtccgg
     1681 gtggccgtct gcatgggctg gacggttgag cgggtggtgg ccgccctggc cgtcctccag
     1741 gctggcggcg cctacgtcac gctcgacccg acctatccgc cggagcgcct ggcctacctg
     1801 atcgccgact cccgggcgcc ggtggtgatc accgaggcac gctaccgcga tcacctcact
     1861 gagaccggcg cgacggtgct gtcgctggac gcggattggg agtcgatcac tggccgcgag
     1921 gacgtcccgc cggagagcgg cgccggcccg gagaacctgg cctacgtcgt ctacacctcg
     1981 ggttccacgg gcgagcccaa gggggtcgag gtccctcacg cggggctgct caacctggtg
     2041 ctctggcacc aggagcggta cgcggtgcac cccgacgacc gggcaggcca ggtggcgagc
     2101 ccggcgttcg atgcctccgt ctgggacctc tggccctacc tggcggccgg cgccgcctcg
     2161 tacatcccgg acgaggagac ccggctgtcg cccacggcca tggttcgctg gtggatggaa
     2221 gaggggatca ccctcacctt cctgccgacg cctctggccg aggcggtgct ggacgaggct
     2281 gtgcctcccg agtcagagct ggcgctgcgt gcccttctgg tcggcggcga ccggctgcat
     2341 cacgctcccg gtccggagtg gccgttccgc ttggtgaacc actacggccc ggcagagtgc
     2401 agcgtggtga gcacggcgct ggacatcccg gccgagccct ggggacaccg cggcggcgtg
     2461 ccccccatcg gccggggcat cgacaccgcc cgcctctacg tcctggatac cgaaggcgag
     2521 ctcgtgccac ccggcgttcc cggcgagctg tatatcggcg gcgctggcct ggcccgcggc
     2581 tacctggggc gtccggagct gacggcggcg gccttcctgc cgaacccatt cgccggcgag
     2641 gcggggtccc ggatgtaccg cacgggcgac cgcgtgcgct ggcgcaccgg cggcgtcctc
     2701 gacttcctcg gccgcttcga ccaccaggtg aaggtgcggg ggatccgcat cgagctcggg
     2761 gagatcgagg ctgtgctggc ccgccacccc gctgtccgcg aggccgcggt ggtgctgctg
     2821 cgcggcggcc gacagggaga ggtccggctc gtcgcctgcg tgacgccggc ggaggagggg
     2881 aggacctcca cggacgagct gcgcgccttc ctgcgcgagc gtctgcccga ggccatggtg
     2941 ccggccgcct tcctgttcgt cgacgccctg cccctgacgc cgaacggcaa ggtggaccgg
     3001 cgggcgttgg aggccctggc tcccggagcg gaggccggca cggagtcgct ctcggcggca
     3061 ccgcgcaccc ccaccgagga gatcatcgcc ggcatctggg ccgacctcct gggccgcgag
     3121 cgtgtcagca cgggcgacga cttcttcgcg ctcggcggcc attcgctcct cgccacccgg
     3181 gtcgtctccc gcgtgcgcga cgccttcggg gtcgagctgc ccctgcggcg cctcttcgac
     3241 gcgccgaccg tcgaggccct cgccgcggcc atcgacgcgg cggtcggcgc ggtgagggga
     3301 gaggccggag ggctgccggt accgccgctc accgccgggc tccgcggtga ccggccgcgc
     3361 ctctcctttg cccaggagcg gctctggttc ctggaccagc tcgatcccag cgccgcttcc
     3421 tatagcgtct ccctggcgct gcggctccat ggcggtctcg accccgaggt gttcgcccgc
     3481 atcctgacgg aggtggtgcg gcgccacgag gtgctgcgca ccaccttcgc gcacgccggc
     3541 ggccagccct accaggtcat cggcccgccg gccgccgtgc cggtgcccgt ggtggacctg
     3601 cgcgggatgt ccggggcgga acgggacgag gaggtggctc tgcgggcgcg ggccgaggcc
     3661 gcgcggccgt tcgatctcgc caacgggccc ctgctgcgcg cgctcctcct gcgcaccggg
     3721 gaggaggact gggtggca
//
LOCUS       DASPJI010000376         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_656379, whole genome shotgun sequence.
ACCESSION   DASPJI010000376 DASPJI010000000
VERSION     DASPJI010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656379"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="VEL74_10045"
     CDS             <1..1495
                     /locus_tag="VEL74_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYG62911.1"
                     /translation="ETALRVALGAGRRDLVRQALAESLPPALAGGALGFLLGIWGTRL
                     LLAAAPPLARPAEIAFEPRGLLFALAACMVVAFLAGLAPALQDAGALREGRARTAGPG
                     GPRGARVLALLVALEVALSLPLLTGAGLLGRSLAHLSSTPLGFTDQGVVTLRLEPPPA
                     RYAGTAELGAFYDRLLARVAAVPGVRAAGLTSSLPLSGLYAITLNVSLEDGERPVEAG
                     YRGVSPGYFAALRIPLRSGRLPGSGDGPDSAKVALVNESFARAAWPASSAIQAVGRSI
                     LLTGAAGMVSREVIGVVGDVRHAGPAEEPRPEIYVPYSQSPLRFATLAARTDRGPEAV
                     LAAVRSAVWEVDRDLALAGVQPLERIVEAATARPRFHRLLAGVLAAVAVALAATGLFG
                     VTAWSVTRRTREIGVRLALGADRGRVVRGVLRGALAPVLAGLVLGLAASLALTRLLAG
                     LLHDVAPTDPVTLSAAPLLLLLIAVAAALLPARRAARTDPAAGLRAE"
     gene            complement(1503..>2794)
                     /locus_tag="VEL74_10050"
     CDS             complement(1503..>2794)
                     /locus_tag="VEL74_10050"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HYG62912.1"
                     /translation="IPPFQTPDEPAHFFHSYAVSEGRLRAVRGVDRVGDVLPASLGVV
                     AYGLLDDVPFHPERRVDPARVRVALEVPLAPERRDFLDFPGSALTSFVPYLPQAAGAL
                     AGRLVGAPPLVLLYLMRLANLLVSTLVIVLAIRLAPGLRWFAAAVALTPMSLCVRSSA
                     SADGLTIAAAFLLAALAARLAWGEGEVRWRDWALLLGSSVLTCLTKAAYFPLMLVALI
                     VPRQRFPWRRRGAVLLVHFLLSGLATAYALDVTRLVDVSLRPGTAVDADRQIRDALAE
                     PWRFAAVVSADYVRHAPRYVAQFVGKLGWADVALPTAFLLAYAALVAALLVLDTSPRI
                     RVKPWQRGALAAAVLLVMGLISASQYAVWTPYRADYIDGIQGRYFLPIATAGAWLLHV
                     ARLGETVPNERLGLGLAVASAVSLAVTVTTVWVRYYG"
BASE COUNT          341 a         1054 c         1041 g          358 t
ORIGIN      
        1 ggagacggcg ttgcgggtgg cgctgggcgc cgggcgccgg gacctggtgc ggcaggcgct
       61 cgccgagagc ctgccgccgg cactcgccgg tggcgccctg ggtttcctcc tggggatctg
      121 gggcacccgt ctcctcctgg ccgccgcccc acccctggcg cggccggccg agatcgcctt
      181 cgagcctcgc ggcctcctct tcgccctggc tgcctgcatg gtggtggcgt tcctggccgg
      241 cctcgcgccc gccctgcagg acgccggcgc gctccgggaa ggccgcgccc gcaccgcggg
      301 cccgggtggc cccagggggg cccgggtgct ggcgctcctg gtggctctgg aggtggcgct
      361 ctcgctgcca ctcctcaccg gcgccggtct cctcggccgg agcctggcgc atctctccag
      421 cacgcctctg ggcttcacgg accaaggggt ggtgacgctc cggctcgagc cgccgccggc
      481 tcgctatgca ggtacggcgg agctgggcgc cttttatgat cggctcctcg cacgcgtcgc
      541 ggccgtgccc ggggtccggg ccgccgggct cacctccagc ctgccgctgt cggggctgta
      601 cgccatcacc ctgaacgtct ctctggagga tggggagcgg cccgtggaag ccggctaccg
      661 cggggtgagc ccgggctact tcgcagcact gcgcattccg ttgcggtcgg gccgccttcc
      721 cggctcgggc gacggtcccg actccgccaa ggttgccctg gtgaacgagt ccttcgcccg
      781 cgccgcctgg ccggcatcat ccgctatcca ggccgtgggg cggtcgatcc tcctgacggg
      841 agcggctggc atggtgagcc gggaggtgat cggcgtcgtc ggggacgtgc ggcacgccgg
      901 cccggccgag gagccgcgtc cggagatcta cgtcccttac tcccagagcc cgctccgctt
      961 cgccaccttg gcggcgcgga cggaccgcgg ccccgaggcg gtgctggccg ccgtccgctc
     1021 ggccgtctgg gaggtggacc gcgacctcgc cctggcgggc gtgcagcccc tggagcgcat
     1081 cgtggaggcc gccacggccc gcccgcgctt ccaccgcctg ctagccggcg tgctcgccgc
     1141 ggtggccgtg gccctggccg cgaccgggct gttcggggtg acggcttggt ccgtgacgcg
     1201 gcggacgcgg gagatcgggg tgcggctggc cctgggagcg gaccgcggcc gggtggtgcg
     1261 cggcgtgctc cgcggcgccc tggccccggt gctcgccggc ctggtcctgg ggctcgccgc
     1321 ctccctggcg ctcacccgcc tgctcgccgg gctcctccac gacgtcgccc ccaccgatcc
     1381 ggtcaccttg agcgcggcgc cgctcctgct ccttctgatc gccgtggccg ccgccctcct
     1441 ccccgcccgc cgcgccgccc gcaccgaccc ggcggcgggt ctgcgggcgg agtagaatcg
     1501 gctcagccgt agtatcgcac ccagaccgtc gtcaccgtta cggcgagcga gaccgcgctc
     1561 gcgacggcca gcccgaggcc gaggcgctca ttcggcacag tctctccgag ccgggcgacg
     1621 tgcagcagcc aggcgccggc ggtggcgatg ggcaggaagt atctcccctg gatgccgtcg
     1681 atgtagtccg cccggtaggg ggtccatacc gcgtattggg aggcgctgat cagccccatg
     1741 accaggagca ctgccgcggc gagggctccc cgttgccagg gtttcacccg gatccggggc
     1801 gaggtgtcga ggaccagcag cgccgcgacc agcgccgcgt aggccaggag gaacgccgtg
     1861 ggcaacgcca cgtccgccca gccgagcttg cccacgaact gcgccacgta gcgcggcgcg
     1921 tggcggacgt agtcggccga gacgaccgcg gcgaaccgcc agggctcggc cagcgcgtcc
     1981 cggatctgcc ggtccgcgtc caccgccgtg cccggccgta gcgagacgtc gacgaggcgg
     2041 gtgacgtcca gggcataggc cgtggcgagg cccgagagca ggaagtggac cagcagcacc
     2101 gccccgcgcc gtcgccaggg gaaacgttgc cgcggcacga tcagcgccac gagcatcaac
     2161 gggaagtagg ccgccttcgt gaggcaggtc agcacggaag agcccagtag tagggcccag
     2221 tcgcgccacc ggacctcgcc ctccccccac gcgagccggg ccgctagggc ggcgagcagg
     2281 aaggcggcgg cgatggtcag cccatccgcc gatgccgagg agcgcacgca gagcgacatc
     2341 ggggtcaggg ccaccgccgc cgcgaaccag cgcagccccg gcgccagccg gatcgccagg
     2401 acgatgacca gcgtggacac caggaggttg gcgaggcgca tgagatagag gagcaccagc
     2461 ggcggcgctc ccacgagcct tcccgcgagg gcccccgccg cctgcggcag gtagggcacg
     2521 aagctggtga gggcggagcc ggggaagtcg aggaagtcgc gcctctccgg cgccagcggc
     2581 acctccagcg cgacccggac cctcgccggg tcgacccgac gctcggggtg gaacggcacg
     2641 tcgtccagca gcccgtaggc caccaccccc aggctcgccg gcagcacgtc gccgacgcgg
     2701 tccacgccgc ggaccgcccg cagccgcccc tcgctcaccg cgtaggagtg gaagaagtgc
     2761 gccggctcgt ccggcgtctg gaaggggggg atga
//
LOCUS       DASPJI010000377         9372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_425473, whole genome shotgun sequence.
ACCESSION   DASPJI010000377 DASPJI010000000
VERSION     DASPJI010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425473"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2423
                     /locus_tag="VEL74_10055"
     CDS             <1..2423
                     /locus_tag="VEL74_10055"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYG62913.1"
                     /translation="ALLLGPVQVIPREMPEIACQSLDVLLPEPGSAAEEDLADDLVAE
                     MAPAAVPPGATAVAFRDGARWLRGFGPARLEGRNEVERLREGGVYLITGGLGGVGLTL
                     AEELAGRTRAKLVLTGRTALPPREEWGAWLTVHGDGDEVSGRIRKLQGLETLGAEVAT
                     VAADVTDPGALAAAVRLAEERFGALHGVIHAAGVPGGGIVHLETAESAERVMAAKVRG
                     TLALEEALAGRRLDFVVLCSSITAVLGDFGQSGYCAANAFLDAYAQAAALRSGRRGPY
                     VVSLGWDRWEEVGMAARSTSPAMSAARPGRHPLLDTLLAETPERDVYLTEMSPERHWV
                     LSEHWIAGRPTVPGTTYLEMARAAFARRAAGRPVEIREAVFLAPLAIEAGAKRQVLTL
                     LEKEGDAFAFRVVSRSTDGGELWQEHARGRIAALEEAAPLAGRRQDPAALLEACAGRE
                     VTSLAKHEGNGNGDGLHDAFLVTGPRWRSLKSLRVADDGQESVAVLDLGEELAGDLEV
                     FSLHPALLDVAAGAVQLLGEGDFLPLAYESLRLHAPLTRRGYSHFRLRGGGAGEILTC
                     DITLLDEEGRELVEIEGFSMRRVGKAAASQLHAGGTGGVRIAGLPALGESPKAAGHGI
                     RPREGMEVFRRVLREGTVPHLVVSTRDLGAAMEEAAARDRSRVAAELEAGAVASSMAV
                     AHARPEVSTSFAPPADDFERRIAQVWESVLGIDSVGVHDNFFELGGTSLTGIQLVTEL
                     KKSLSVDLPTVSIFEAPTVAALARYLRPAGGEAGSVASALGQTRSRAEKKRQALYQAQ
                     AQAKKRRR"
     gene            2420..7111
                     /locus_tag="VEL74_10060"
     CDS             2420..7111
                     /locus_tag="VEL74_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016866914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYG62914.1"
                     /translation="MSEPEELETGGESRDIAVIGMAGRFPRAARLEDFWRNLADGVEA
                     ITFLSDEELAAAGVSRELLQHPNYVKAASLLDGVDTFDAPFFGYSAREAEIMDPQQRL
                     FLEHAWEALEGAGYVPERFEGLIGVYAGVAWNTYLLSNLTSNRELFEGGGGFQVFITN
                     DKDFMPTRVSYKLNLKGPSVVVQTSCSTSLVAIHLACLSLLNYECDMALAGGVTVRVP
                     QKEGYFHHEGGLSSPDGHCRAFDARAAGTIFGSGIGVVVLKRLSEALDDGDPIRAVIK
                     GSAINNDGSVKVSYTAPSVEGQAGVIAAAHAIAGFESGEDLETLRYVECHGTGTSLGD
                     PIEVTALTKAFREHTDRRAFCALGSVKTNLGHLDAAAGVAGFIKTVLALEHRQLPPSL
                     HFEEPNPVIDFANSPFFVNTALREWETEDGAPRRAGVSSFGVGGTNAHVILEEAPEPE
                     PSGPSRPWQLLRLSARSETALQAATANLRAWLEENEEASLPDVAYTLALGRSVFRHRQ
                     VLVCRDRDEALSLLAGENAPRLLTETDAEDPRDRPVVFLFSGQGSQHHGMARELSETE
                     AVFRQEMDRCAELLRPRLGLDLRRVLWPAPGSEEEAARQLARTALTQPALFAVEYALA
                     RLWMSWGVRPQAMLGHSIGEYVAACLAGVFSLEDALALVAARGRLMQDLPAGGMLSVP
                     LPEEELAPLLGPHLSIAAVNEPARTAVSGPFGALEELERDLTARGVEYRRLQTSHAFH
                     STMMEPALQPFLAEVRRLRLQPPAVPFLSNVTGTWITPEQATDPEYWARHLRQPVRFA
                     AGVAELLRDPRRILLEVGPGRTLATLAARHPGRTGQPVLASLRHPQDDTSDLRMMLEA
                     LGRLWLSGLAVDPAAFYGGEHRRRVALPTYPFERQRYWIDPKPLALVEPAASRPGTPV
                     KRADLADWFYLPSWKPSLATGTAESTGPRRWLVLAHRSGPSARLAELLSHRQAEVVTV
                     HPGDDFEQRLASLASDAPLTVLHAWALGWDLPAASDAPGFEAAQEQGFHALVSLARTL
                     GRRPGPVDLLVVADGLAALTAAEPTAPEKATLLGLLKVLPQELPHVTCRAIDVALPPS
                     GSRQESALLERLLAEISHSPSDELVVLRGRERWVPDFEPVRLDSRSTLLREGGVYLLT
                     GALAGNGFALARLLARDARARLVLLEPPSTPGGGGGPDRPAQATGSVGAGEGGAHTKE
                     PSRDDPAPRAGGDRHARIGLLESLGASVLLLETDLADESDLRRALAEAEAHFGPLHGV
                     IHAASTQGERTFRALAELGPEEAAWHFRPKAHALYSLDRVLAGRHLDFFAVISSLATV
                     LGGVAYGAYAAANAFLDAFVRERNRQEGSSWLALDWDVWQFEDETEQILAVREDLAGL
                     AMTPREGEETFRRALAAPADRLLVSTADLAARLAQRRERIEAARGRHTQVPSQGTAGT
                     MTSKALHPRPLQTPYTPPETELERRIVEVWQRTLGFEQIGVHDNFFELGGDSFLAIQV
                     VAKIKEELQVDLPVARLYQGLTVRALAILLDEEKAAGQRAAQLEERRESMGKRKQFLE
                     QRRAGRKTEVS"
     gene            7113..>9372
                     /locus_tag="VEL74_10065"
     CDS             7113..>9372
                     /locus_tag="VEL74_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HYG62915.1"
                     /translation="MSADETQYDGSEIAIIGMACRFPGASTPEAFWRNLVAGVESLTT
                     LSEEELLAAGVPREALRDPRYVPRAGFAEGIEYFDPAFFGYTPLEAKIMDPQHRLFLE
                     CAWEAFEHAGYNPEGNRDRVGVFTGAKTNTYLFSLFSNRELFRTLDSFQLALGNDLAA
                     MATRISYKFNLKGPSYGLHTACSTSLVAVHLACQSLLLGECRMALAGGSAVNIPYRTG
                     YLYQKGGILSPDGSCRTFDEKAQGSNFSNGSGAVLLKRLEDALADGDHVHAIIRGTAS
                     NNDGSAKASFTAPGVEGQADLLIDALACAGVDADTISYIEAHGTGTDLGDTIEMLALT
                     EAFRASTPRRGFCAIGSVKTNVGHLEVAAGVAGLIKTALALEHRQIPPSLHYEKPNPR
                     IDFESSPFYVATQLTGWQSDGPRRAGVSSFGIGSTNAHVIMEEPPPAVPVTPSRPWQL
                     LLLSARSEPALNTMTANLARRLESLTDTDLPDAAYTLQTGRKDFGWRRAVLCRGTADA
                     VAALSGADPERVLTHLREPADLPVAFLFPGLGDHHAEMGLGLYRTERVFREELDRCAE
                     LLRPHLGVDLREVLYPRGTDAGEAGTGKLDLRRMLGRGEKETGPRRIDETWLAHPALF
                     AVEYALARLWMSWGIVPQSLVGYSLGEYVAACLAGVLELPCALALVARRARLIQDLEP
                     GAMLAVSLPEAELMPHLESHGLSLAAVNGPAICVAAGPVTAVDTLERDLGALGVACRR
                     LPTHHAFHSSMMA"
BASE COUNT         1269 a         3502 c         3265 g         1336 t
ORIGIN      
        1 aggcgctgct cctcggtccg gtgcaggtga tccccaggga gatgccggag atcgcctgcc
       61 agagcctgga cgtgttgctg cccgagccgg ggagcgccgc cgaggaggat ctggcggacg
      121 acctcgtggc cgagatggcc ccggcggcgg tcccccccgg cgcgaccgcc gtggcgttcc
      181 gcgatggcgc ccgctggctg cgcggattcg ggccggcgcg cctggaggga cggaacgagg
      241 tcgagcgcct ccgggagggc ggcgtctacc tgatcaccgg cggtctcggc ggcgtgggcc
      301 ttaccctggc cgaggagctg gccggccgga cgcgggcgaa gctcgtcctc accggccgga
      361 cggccctgcc gccgcgcgag gagtgggggg cctggctcac cgtgcacggc gacggcgacg
      421 aagtgagcgg ccgcatccgc aagctccagg gtctggagac gctgggagcc gaggtcgcca
      481 ccgtcgcggc cgacgtcacc gatccggggg ccctggccgc ggcggtgcgc ctggccgagg
      541 agcgcttcgg cgccctgcac ggcgtgatcc acgccgccgg cgtgccgggc ggcggcatcg
      601 tccacctgga gaccgccgag tccgccgagc gcgtgatggc ggccaaggtc cgggggacgc
      661 tggcgctgga ggaggccctc gccgggcggc ggctcgactt cgtggtcctc tgctcgtcga
      721 tcaccgcggt gctgggcgac ttcggccaga gcggctactg tgcggccaac gccttcctgg
      781 acgcctatgc ccaggccgcc gccctgcgct ccggccggcg cggcccctac gtcgtctccc
      841 tcggctggga ccgctgggag gaggtgggca tggcggcccg ctccacctcg cccgcgatgt
      901 ccgcagcgag gccggggagg cacccgctgc tggacaccct gctcgccgag acaccggagc
      961 gggacgtcta cctcaccgag atgagccccg agcgtcactg ggtgctctcc gagcactgga
     1021 tcgccggccg tcccaccgtg cccggcacca cctatctgga gatggcccgc gccgccttcg
     1081 cccgccgcgc cgccggccgg ccggtggaga tccgcgaggc cgtcttcctc gctccgctgg
     1141 cgatcgaggc cggggcgaag cgccaggtgc tgaccctcct ggagaaggag ggcgacgcct
     1201 tcgccttccg tgtggtcagc cggagcacgg acggaggcga gctgtggcag gagcacgccc
     1261 gcggccggat cgccgcgctg gaagaggccg cgccgttagc aggccgccgg caggatccgg
     1321 cggccctgct ggaggcctgc gccggacggg aggtcacctc cctggcgaag cacgagggca
     1381 acggtaacgg cgacggcctc cacgacgcct ttctggtaac ggggccgcgc tggcgcagcc
     1441 tcaagagcct gcgggtggcg gatgacggcc aggagagcgt cgcggtgctc gacctcggcg
     1501 aggagctggc cggtgacctg gaggtcttct ccctccaccc cgccctgctg gacgtggcgg
     1561 ccggcgcggt gcagctcctg ggagaggggg atttcctccc gctggcctac gagagcctgc
     1621 gcctccacgc gccgctcacc cgccggggct acagccattt ccgtctgcgc ggcggcgggg
     1681 ccggcgagat cctgacctgc gacatcactc tcctggacga ggaggggcgc gagctggtgg
     1741 agatcgaagg cttctccatg cgccgggtcg gcaaggccgc ggcttcccag ctccacgcgg
     1801 gtgggacggg gggtgtgcgg atcgccgggc tgccggccct cggggaatct cccaaggcgg
     1861 ccggtcacgg catccggccg cgagagggga tggaggtttt ccggcgcgtg ctgcgggagg
     1921 ggaccgtgcc ccacctggtg gtctccaccc gcgatctggg ggcagccatg gaagaggccg
     1981 cggcccgcga ccggtcacgg gtggccgcgg agctggaagc gggcgccgtt gcctcttcca
     2041 tggccgtcgc ccacgcgcgg ccggaggtga gcacttcctt cgcgccgccg gccgacgact
     2101 tcgagcgccg catcgcccag gtctgggaga gcgtcctcgg cattgacagt gtgggcgtgc
     2161 atgacaactt cttcgagctg ggcggcacct ctcttacagg gatccagctg gtcaccgagc
     2221 tcaagaagag cctgagcgtc gacctgccga cggtgagcat cttcgaggcc cccaccgtgg
     2281 cggccctggc ccgctacctc cggccggcgg gcggcgaggc aggcagcgtg gcctccgcgt
     2341 tggggcagac ccgcagccgg gccgagaaga agaggcaggc cctatatcag gcccaagcgc
     2401 aggcgaagaa gaggagacga tgagcgagcc ggaagagttg gagacaggag gggagagccg
     2461 ggacatcgcc gtcatcggca tggcggggcg cttcccgcgg gccgcgcgcc tggaggattt
     2521 ctggcgcaac ctggcggacg gcgtcgaagc catcaccttc ctctccgacg aggagctggc
     2581 cgcagcgggc gtctcccggg agctgctgca gcaccccaac tacgtcaagg ccgccagcct
     2641 cctcgacggc gtcgacacct tcgacgcgcc gttcttcggc tacagcgccc gcgaggccga
     2701 gatcatggac ccccagcagc gcctcttcct ggagcacgcc tgggaggcgc tggagggggc
     2761 cggctacgtc cccgagcgct tcgaggggct catcggcgtc tacgccggcg tcgcctggaa
     2821 cacctacctg ctgtccaatc tcacctccaa ccgcgagctg ttcgaggggg gcggcggctt
     2881 ccaggtgttc atcaccaacg acaaggactt catgcccacc cgggtctcct acaagctgaa
     2941 cttgaagggg cccagcgtcg tggtgcagac gagctgctcc acctctctgg tggcgatcca
     3001 cctcgcctgc ctgtcgctgc tcaactacga gtgcgacatg gcgcttgccg gcggcgtcac
     3061 cgtgcgggtg ccgcagaagg aagggtactt ccatcacgaa ggtgggctct cctccccgga
     3121 cggccactgc cgcgccttcg acgcccgggc cgcgggcacc atcttcggca gcggcatcgg
     3181 cgtggtggtg ctgaagcgcc tgtccgaggc gctggacgac ggcgacccca tccgcgcggt
     3241 gatcaagggc tcggcgatca acaacgacgg ttcggtcaag gtgagctaca ccgcgccgag
     3301 cgtcgagggg caggccgggg tcatcgccgc ggctcacgcc atcgctggct tcgagtccgg
     3361 cgaggacctc gagaccctcc gctacgtcga gtgccacggc accggcacct cactggggga
     3421 cccgatcgag gtcacggcgc tcaccaaggc gtttcgtgag cacacggacc ggcgcgcctt
     3481 ctgcgccctg gggtcggtca agacgaacct cgggcacctg gacgccgcgg cgggggtcgc
     3541 cggcttcatc aagacggtgc tggccctgga gcaccggcag ctccccccca gcctgcactt
     3601 cgaggagccg aacccggtca tcgacttcgc caacagcccg ttcttcgtca acaccgcgtt
     3661 gcgcgagtgg gagacggagg acggcgcgcc gcggcgggcg ggggtgagct ccttcggcgt
     3721 cggcggcacc aacgcccacg tgatcctcga ggaggcgccg gagcccgagc cctcggggcc
     3781 gtcccgcccc tggcagctcc tgcgcctgtc cgcccgcagc gagacggctc tccaggcggc
     3841 gacggccaac ctccgggcct ggctcgagga gaacgaagag gcgagccttc cggacgtggc
     3901 ctataccctg gccttgggcc gttcggtctt ccggcaccgc caggtcctgg tctgccgcga
     3961 ccgtgacgag gccctgagcc tgctcgcggg ggagaatgcc ccgcgcctcc tcaccgagac
     4021 cgacgccgag gacccgcgcg accggccggt ggtcttcctc ttctccggcc agggctcgca
     4081 gcatcacggc atggcccgcg agctgtccga gaccgaggcc gtgttccggc aagagatgga
     4141 ccgctgcgcc gagctgctgc ggccccgcct ggggctcgac cttcggcggg tgctgtggcc
     4201 cgcccctggc agcgaggagg aggcggcgcg ccaactcgcc cgcaccgccc tcactcagcc
     4261 ggccctgttc gctgtcgagt acgccctggc ccgcctgtgg atgagctggg gggtgcgtcc
     4321 ccaggccatg ctcggccaca gcatcggtga atacgtggcg gcctgcctcg ccggcgtctt
     4381 ctcgctggag gacgcgcttg ctctggtggc cgcccgcggc cggctgatgc aggacctgcc
     4441 ggcgggaggg atgctgtcgg tgccgctgcc cgaggaggag ctggcgccgc tcctggggcc
     4501 gcacctctcc atcgccgcgg tcaacgagcc ggcgcgcacg gcggtgtcgg ggccgttcgg
     4561 tgcactcgaa gagctggagc gtgatctcac ggcgcgcggc gtggagtacc ggcgcctgca
     4621 gacctcccac gctttccact cgacgatgat ggagcccgcg ctccagccct tcctcgccga
     4681 ggtgcggcgg ctgcgcctgc agccgccggc ggtccccttc ctctccaacg tcaccggcac
     4741 ctggatcacg ccggagcagg cgaccgaccc ggagtactgg gcccgccacc tgcgccagcc
     4801 ggtgcgcttc gcggccggcg tggccgagct gctgcgcgac ccccggcgca tcctcctgga
     4861 ggtggggccg ggccgcaccc tggcgacgct ggcggcccgt caccccggcc ggaccggcca
     4921 gcccgtgctc gcatcgctcc gccacccgca ggacgacacc tccgacctgc ggatgatgct
     4981 cgaagccctg ggccgcctct ggctctccgg tctggccgtg gatcccgcgg cgttctacgg
     5041 cggcgagcac cggcggaggg tggcgctgcc cacctatccc ttcgagcgcc agcgctactg
     5101 gatcgatccc aagcccctgg ccctcgtcga gcccgccgct tcgcggcccg gtactccggt
     5161 caagcgcgcc gacctggccg actggttcta tctgccgtcc tggaaaccgt cgcttgccac
     5221 cggaaccgcg gagagcacgg gtccgcgccg ctggctcgtg ctggcccacc ggtccggtcc
     5281 ctcggcacgg ctcgccgagt tgctgtctca ccggcaggcc gaggtggtga cggtccatcc
     5341 cggcgacgat ttcgagcagc gacttgccag tctcgcgagc gacgcgcctc tcactgtcct
     5401 tcacgcctgg gccctcggct gggacctgcc cgccgcctct gacgcccccg gcttcgaggc
     5461 cgcccaggag cagggcttcc acgccctggt ctccctcgcc cgaacgttgg gccgtcgtcc
     5521 cggtcccgtc gatctcctgg tggtggccga cggcctcgcc gccctcaccg ccgcggagcc
     5581 gaccgcgccg gagaaggcca ccctgctcgg ccttctcaag gttctccccc aggagctgcc
     5641 ccacgtcacc tgccgggcca tcgacgtggc gctgccaccc tccggctccc gtcaggagtc
     5701 cgccctcctg gagcgcctcc tggccgagat ttctcactca ccctccgacg agttggtcgt
     5761 cctgaggggc cgcgagcgct gggtccctga cttcgagccc gtccgtctcg actccaggtc
     5821 tacgctcctc cgcgaagggg gcgtctatct cctcaccgga gctctggccg gcaatggctt
     5881 cgccctggcg cgcctcctgg cccgtgacgc ccgggcccgc ctcgtcctcc tggaacctcc
     5941 atccacgccg ggtgggggag gggggccgga tcgtcccgcg caggcgactg gcagcgtggg
     6001 ggccggggag ggcggagcgc acacgaagga gccgagcagg gacgatccgg ccccccgggc
     6061 ggggggcgac cgccacgctc gaatcggcct cctcgaatcc ctgggagcct ccgtcctcct
     6121 cctggagacc gatctggccg acgagtccga cctccgccgc gcgctggccg aggccgaagc
     6181 ccacttcggc cccctgcacg gcgtcatcca cgccgccagc acccaggggg agcggacctt
     6241 ccgcgccctg gccgaactgg gacccgagga agccgcctgg cacttccggc ccaaggccca
     6301 cgccctgtac tccctggacc gggtcctggc cggccgtcac ctggacttct tcgccgtgat
     6361 ctcctccctg gccaccgtcc tgggaggcgt ggcgtacggc gcgtacgcgg cggccaacgc
     6421 tttcctggac gccttcgtcc gcgagaggaa tcgccaagag ggttcctcct ggctcgccct
     6481 ggactgggat gtctggcagt tcgaggacga gaccgagcag atcctggcgg tgcgtgaaga
     6541 cctcgccggc ctcgccatga ctccgcgcga gggggaggag accttccggc gggccctcgc
     6601 cgcgccagct gaccgcctcc tcgtctccac cgcggacttg gcggcgcgcc tcgcccagcg
     6661 gcgggagcgg atcgaggccg cccgcggccg ccacacccag gtccccagcc aggggaccgc
     6721 gggcaccatg acctcgaagg ccctccatcc gcgccccctc cagactccgt acacgccccc
     6781 ggagaccgag ctggagcggc gcatcgtcga ggtctggcag cggaccctgg ggttcgagca
     6841 gatcggcgtc catgacaatt tcttcgagtt gggtggagac tccttcctcg ccatccaggt
     6901 ggtggcgaag atcaaggaag agctgcaggt ggacctcccc gtcgcccggc tctatcaggg
     6961 gctgaccgtc cgcgccctgg ccatcctcct cgacgaggag aaggcggccg gacagcgggc
     7021 ggcgcagctc gaagagcggc gcgagagcat ggggaagcgc aagcagttcc tggagcagcg
     7081 ccgtgcaggc agaaagaccg aggtgagctg agatgtccgc agacgagacc cagtacgacg
     7141 gctccgagat cgccatcatc ggaatggcct gccgcttccc cggcgcctcc actcccgagg
     7201 cgttctggcg caacctggtg gccggggtgg agtccttgac gacgctcagc gaggaggagc
     7261 tgctggccgc cggcgtgccg cgcgaggccc tgcgcgaccc gcgctacgtc ccccgcgccg
     7321 gctttgccga ggggatcgag tacttcgatc ccgccttctt cggctacacg ccgctcgaag
     7381 ccaagatcat ggacccccag caccgcctct tcctggagtg cgcctgggag gccttcgagc
     7441 acgccggcta caaccccgag gggaaccgcg accgggtggg cgtcttcacc ggcgccaaga
     7501 ccaacaccta cctcttcagc ctgttctcca accgcgagct cttccgcacc ctcgacagct
     7561 tccagcttgc cctgggcaac gacctggcgg cgatggcgac tcgcatctcc tacaagttca
     7621 acttgaaggg gccgagctac ggcctgcaca ccgcctgctc gacatcgctc gtggccgtcc
     7681 acctcgcctg ccagagcctg ctgctcggcg aatgccgcat ggcgctcgcc ggcggctccg
     7741 cggtcaacat tccgtaccgc accggctacc tctatcagaa gggggggatc ctctcccccg
     7801 acgggagctg ccgcaccttc gacgagaagg cccaagggag caacttcagc aacgggtcgg
     7861 gcgcggtgct gctcaagcgc ttggaggacg ccctcgccga cggcgaccac gtgcacgcca
     7921 tcatccgcgg cacggcgagc aacaacgacg gctccgccaa ggcgagcttc acggcgcccg
     7981 gcgtcgaggg gcaggccgac cttctcatcg acgccctggc ctgcgccggc gtcgacgccg
     8041 acaccatctc ctacatcgag gcccacggta ccggcaccga cctcggcgac accatagaga
     8101 tgctggcgct taccgaggcg ttccgcgcct ccaccccacg ccggggcttc tgcgccatcg
     8161 gctcggtgaa gaccaacgtc ggtcatctgg aggtcgccgc cggcgtcgcc gggctcatca
     8221 agacggcgct cgccctggag cacaggcaga tcccccccag cctccactac gagaagccga
     8281 acccgcgcat cgacttcgag tcgagcccgt tctatgtcgc cacccagctc accggttggc
     8341 agtccgacgg cccgcgccgc gccggcgtca gctcgttcgg catcggcagc accaacgccc
     8401 acgtgatcat ggaggaaccc cctccggcgg tcccggtgac gccgtcgcgc ccctggcagc
     8461 tcctgctgct gtccgcccgc agcgagcccg cgctgaacac catgacggcg aacctggcgc
     8521 ggcggctgga gtcgctcacc gataccgacc tgccggacgc ggcctacacg ctgcagaccg
     8581 gccgcaagga cttcggctgg cgccgcgccg tcctctgccg tggcaccgcc gacgcggtgg
     8641 cggcgctctc gggtgcggac cccgagcggg tgctcaccca tctgcgcgag ccggccgacc
     8701 tccccgtcgc cttcctcttc cccggcctgg gggatcacca cgcggagatg ggcctcgggc
     8761 tctaccgcac cgagcgggtg ttccgggagg agctcgaccg ctgcgccgag ctgctccggc
     8821 cccacctcgg cgtcgacctg cgggaggtgc tctacccacg cggcaccgac gcgggggagg
     8881 cggggaccgg caagctcgac ctgcggcgca tgctcggccg cggggaaaag gagacggggc
     8941 cgcggcgcat cgacgagacc tggctcgccc atcccgccct cttcgccgtc gagtatgccc
     9001 tcgcccggct gtggatgtcc tggggcatcg tgccgcagtc gctggtgggc tacagcctcg
     9061 gcgagtatgt cgccgcctgc ctggccggcg tgctggagct gccgtgcgcc ctcgctctgg
     9121 tggcgcggcg ggcgcggctg atccaggacc tcgagcccgg cgccatgctg gccgtctcgc
     9181 tgcccgaggc ggagctgatg ccccacctgg agagccacgg cctctccctc gccgcggtca
     9241 acggcccggc gatctgcgtc gccgccgggc ctgtgacggc cgtggacacg ctggagcgcg
     9301 acctcggcgc gctgggggtg gcctgccgcc gcctgcccac ccaccacgcg ttccactcca
     9361 gcatgatggc ga
//
LOCUS       DASPJI010000378         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_138053, whole genome shotgun sequence.
ACCESSION   DASPJI010000378 DASPJI010000000
VERSION     DASPJI010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138053"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VEL74_10070"
     CDS             complement(<1..752)
                     /locus_tag="VEL74_10070"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYG62916.1"
                     /translation="MLFLVLILPVAVSAQTAPPPSSTPASPATPATTSAEPVTLTLAE
                     ALQMAVTQNPGIGRSRAEVSVLEAQEDVVFSAILPKINFQGNYTRNDREVAFGSGEDS
                     TTILPENDWSYRLTLSQPIYAGNRERKALQQARLGIQSARQGLSGTTDQVIASVVANY
                     LTVLQAEDLVAVEQRNLELAGRRRDQAQIFFEAGETTRVDVLRAEADSKEVERRLATA
                     RQIREQAVGRLRLDLALDAPIRVTEPGDLFPAL"
     gene            complement(792..1445)
                     /locus_tag="VEL74_10075"
     CDS             complement(792..1445)
                     /locus_tag="VEL74_10075"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HYG62917.1"
                     /translation="MTETLSPTTEQVDTREALLDAAESLFSELGIQASSLRAITQRAG
                     ANLAAVHYHFGSKEGLVRAVFSRRLKPLNEQRLRLLEACDLTADDAVEQVLNAFLSPL
                     LRMSRETPEGVRDFARLMGRAYMEPSEEVREMLLEEIGEVVRRFTEALGRILSPLPGQ
                     ELLWRLHFVAGAMGHTVACSHALEKHSHGLCQPSNPDEALRFMIPFLAAGLRAGTTP"
     gene            complement(1577..1792)
                     /locus_tag="VEL74_10080"
     CDS             complement(1577..1792)
                     /locus_tag="VEL74_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62918.1"
                     /translation="MRKRTTKLSLHRETLRHLDPMQKVAGGVGTSEECLAPTGCECPG
                     TVGCVAPTVFHTCGTTCTVPTGYHQCG"
     gene            complement(1927..>2450)
                     /locus_tag="VEL74_10085"
     CDS             complement(1927..>2450)
                     /locus_tag="VEL74_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HYG62919.1"
                     /translation="APKATLMSYIIKGILDRQLPWGLVLLGVMIAVVLEMSGIPSLAF
                     AVGVYLPLSASTPILAGGIVRWLVDRHLRRKLAHRKLTEEELAAEGDKSPGVLMASGY
                     IAGGAIAGIAIAIMASQMAGFSDRITRFMEGSNPFFAGPWADALSLIPFAVICLLLYL
                     AGRELILAGRPKS"
BASE COUNT          395 a          807 c          877 g          371 t
ORIGIN      
        1 agggccggga acaggtcccc cggctcggtc acccggatgg gggcgtcgag ggcgaggtcg
       61 agccgcagcc ggccgaccgc ctgctcgcgg atctgccgcg ccgtggccag gcgccgctcg
      121 acctccttcg agtccgcctc ggcgcgcagg acgtcgacgc gggtggtctc gccggcctcg
      181 aagaagatct gcgcctggtc gcgccggcgg ccggcgagct ccaggttgcg ctgctcgacc
      241 gcgacgaggt cctcggcctg cagcacggtg aggtagttgg cgacgacgct ggcgatcacc
      301 tggtccgtgg tgccggagag cccctggcgg gcgctctgga tgccgagccg ggcctgctgg
      361 agcgccttgc gctcgcggtt gccggcatag atcggctgcg acagggtcaa ccggtagctc
      421 cagtcgttct cgggcaggat cgtggtcgag tcctcccccg agccgaaggc gacctcgcgg
      481 tcgttgcggg tgtagttgcc ctggaagttg atcttcggca ggatcgccga gaagaccacg
      541 tcctcctggg cctcgagcac cgacacctcg gcgcgcgacc ggccgatgcc ggggttctgg
      601 gtcacggcca tctgaagggc ctcggccaga gtcagggtca ccggctcggc cgacgttgtt
      661 gccggggtgg ccgggctggc cggggtggac gaaggcggcg gcgccgtctg cgcggacacc
      721 gccaccggca gtatcagaac aaggaacagc aaggttccga gcctcaaaag cgtatctcct
      781 tggaaagggg cctagggggt cgtgcccgcc cgcaggcccg cggcaagaaa cgggatcatg
      841 aagcgcagcg cctcgtccgg attggacggc tggcagagcc cgtgggagtg cttctccagg
      901 gcatggctgc aggccacggt gtggcccatg gcgccggcga cgaagtggag ccgccagaga
      961 agctcctggc ctggcagggg agagaggatc cggcccaagg cttcggtgaa gcggcggacc
     1021 acttcgccga tctcctccag cagcatctcc cgcacctcct cggagggctc catgtaggcc
     1081 cggcccatca gccgggcgaa gtcccgcacc ccctccggcg tctcgcggga catccggagg
     1141 agcggcgaca ggaacgcgtt cagcacctgc tccaccgcgt cgtccgcggt gaggtcgcag
     1201 gcttcgagca ggcgcaggcg ctgctcgttc agcggcttga ggcgccgcga gaagacggcg
     1261 cgcaccagcc cctccttgga gccgaagtgg tagtgcacag ccgccaggtt cgcccccgcc
     1321 cgctgggtga tggcgcgcaa agacgacgcc tggatgccca gctcggagaa gaggctctcc
     1381 gcggcatcca gcagcgcctc acgggtatcg acttgctctg tcgtagggct cagggtctca
     1441 gtcatgattc aaacgtccgt ttcaaacaga cgtttgaatc atagcgggat tcggctggct
     1501 gtcaaccccc ctgaagcgga aaggccctcc accccggagg gcggagggcc tggaggtcaa
     1561 aggcgggagc ccgccctcaa ccgcactggt ggtagccggt cggcacggtg caggtcgtcc
     1621 cacaggtgtg aaacacggtg ggagccacgc atccgaccgt gcccgggcat tcgcagccgg
     1681 tcggcgccag gcactcttcg ctggtgccga caccgcccgc caccttctgc atcgggtcga
     1741 ggtgacggag ggtctcgcga tggagcgaca gcttggtggt cctctttctc attgagcatt
     1801 actcctattt acggtcaagc cagccgtagc cggcgggtaa acccgtccag agtgcagagc
     1861 cgttcttgga tgaacctagg gctctgactc tatacgacgg cgcccaccct ttattacacc
     1921 gctgcttcag ctcttcggcc ggccggcgag gatgagctcg cgcccggcca ggtagagcag
     1981 caggcagatc acggcgaacg ggatcagcga cagggcgtcg gcccaggggc cggcgaagaa
     2041 ggggttggag ccctccatga agcgggtgat ccggtcgctg aagccggcca tctggctggc
     2101 catgatggcg atggcgatac cggcgatggc gccgccggcg atgtagccgg acgccatgag
     2161 cacgccgggg ctcttgtccc cctcggcggc aagctcctcc tcggtcagct tgcggtgcgc
     2221 cagcttgcgg cgcaggtggc ggtcgaccag ccagcggacg atgccgccgg cgaggatcgg
     2281 cgtcgacgcc gagagcggca ggtagacgcc gaccgcgaag gcgagggagg ggatgcccga
     2341 catctccagc acgacggcga tcatcacccc caggagcacc aggccccagg ggagctgccg
     2401 gtcgaggatg cccttgatga tgtaggacat cagcgtcgcc ttcggcgcgt
//
LOCUS       DASPJI010000379         2966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_138207, whole genome shotgun sequence.
ACCESSION   DASPJI010000379 DASPJI010000000
VERSION     DASPJI010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138207"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="VEL74_10090"
     CDS             complement(<1..1197)
                     /locus_tag="VEL74_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62920.1"
                     /translation="MTRLRLLRPLLCLLSILAAAPVPSAVAGTGFWSPLGPEGASVTA
                     LVVDPTGAVYAAARRGGIYKSSTGGDTWGLLSRGTGSETIVALAATPRPFGPIYAAGE
                     SGAVFKTVDGGGNWVRVWAGAPQPDGNVAATFSLATDARRPDTLFAGTRQGVYRSTDG
                     GITWARRGNLAGAVRSLANDAAGGWLYAGVDGQGVFRSFNQGGTWERISAGLPPNPFF
                     FSLAFDPDRPRTLLATTPAGLYRSVDRGRRWQRVAGAPDVNALAVVFQRGGRAYAAFT
                     GGIFYSTDSGATWRRSANLPGRVFGDPRSLAAGTDRVFVGVLGDSDGPGLYRSLDFGA
                     TFQPAIEDLQGLAVTTVAVAPSDPETIYAAVSNSLFKTTDGGTNWTRVSIPHLESSFY
                     PISDILV"
     gene            1794..2165
                     /locus_tag="VEL74_10095"
     CDS             1794..2165
                     /locus_tag="VEL74_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62921.1"
                     /translation="MALEKARRQTEEISAAAAALERVMPGVEVNLSGVTGGPSEVVDR
                     RGALTGAAPGAGSETIVREFLGRHSALTGSRPPTSRTSWSSATGPAARAVCACCAWSS
                     GSTAVPSSRARPASSSTVKAG"
     gene            2233..2568
                     /locus_tag="VEL74_10100"
     CDS             2233..2568
                     /locus_tag="VEL74_10100"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HYG62922.1"
                     /translation="MADPAGANGDVPTGNQTTLQLVIDGLKMTPVDPAFTDGRDAILD
                     ADCATNACAHEDFIWQTSPSAGSAGERRSRAGEARLIPPGAAVAPAERPSSPLVWPPY
                     PANQPPYPR"
BASE COUNT          444 a         1009 c         1073 g          440 t
ORIGIN      
        1 gaccaggatg tcgctgatcg gatagaagct gctctccagg tgggggatgg agacgcgggt
       61 ccagttggtt cccccatccg tggtcttgaa gaggctgttg gagaccgcgg cgtagatcgt
      121 ctcgggatcg gagggggcca cggccacggt ggtcacggca agcccctgga ggtcctcgat
      181 cgccggctgg aaggtggcgc cgaagtccag gctgcggtag agcccggggc cgtcggagtc
      241 gcccaggacg ccgacaaaga cccggtccgt tcccgcggcg agggacctgg ggtctccgaa
      301 gacgcggccc ggcaggttgg ccgagcgccg ccaggtggcg ccggaatccg tgctgtagaa
      361 gatcccgccg gtgaacgccg cataggcccg gccgccacgc tggaaaacga cggccagagc
      421 gttgacgtcg ggcgcgccgg caaccctctg ccacctccgg ccacggtcga cgctccggta
      481 gaggcccgcc ggtgtggtgg ccagcagcgt ccttggccgg tccgggtcga aggccagtga
      541 gaagaagaag gggttcgggg ggaggccggc gctgatccgc tcccaggtgc cgccctggtt
      601 gaagctccgg aagacgccct ggccgtcgac gccggcatag agccagccgc ccgcggcgtc
      661 gttggccagc gagcgcaccg cgcccgcgag gttgccgcgg cgggcccagg tgatcccccc
      721 gtcggtcgag cggtagaccc cctggcgcgt cccggcgaac agagtgtccg gccgccgcgc
      781 gtcggtcgcc agcgagaacg tggcggcgac gttgccgtcg ggctgcggcg cgcccgccca
      841 gacacggacc cagttgccgc cgccgtcgac ggtcttgaag acggcgccgc tctcgccggc
      901 ggcatagatg gggccgaagg ggcgaggggt ggcggcgagg gcgacgatcg tctcggagcc
      961 ggtaccgcgg ctcaggagcc cccaggtgtc gccgccggtg gagctcttgt agatgccccc
     1021 ccgccgggcg gcggcataga cggcgccggt cgggtcgacc accagcgccg tcaccgacgc
     1081 gccctcgggg ccgagcgggc tccagaagcc ggtgccggcg accgcggagg ggacgggagc
     1141 cgcggcgagg atcgacaaca ggcagaggag ggggcgcagc agtcgcagtc gagtcatgtt
     1201 tcagctccta gaagaagatc agttgtcgaa ggcctccatc taattgtacg gatgaggagc
     1261 ctcgcgcgtt gtgcgagcga cgatgcggag gcgacgatcc acctctatat aatgtcgtca
     1321 ctcgctcctg aggtcccagc gtctcgtctg tccgctcgtc ctgctcgccc tcctgccggc
     1381 cgcctcggcg gcggcctccg gttccaatcc ctcccggttc gcctccctgg acggcatcac
     1441 cctgttcacc gccgacgacg gcgtccacgg cctccgcggt gctctggaaa cttcccgcgg
     1501 cgtccgtctg aagagatagc cagccggcgg ctcaaccttt tcgccgccga tggtgtctct
     1561 tcatcaggaa gggagaacct gccgtctcct ggccctgaag aggcgcgggg cacttccgat
     1621 gcggtagcga tcggacgggg aagtgcccgg cgcccttcag ggctcatgct tcttctctcg
     1681 ccagagcctc cggctctccc tcctcccaac ccccagggca aggtgtaggt gatcaggctg
     1741 cccaacgcag gggagccggc cgcgggggcc aagtcggcgc ggcccgaggc ccgatggccc
     1801 tcgagaaggc ccgccgccag accgaggaga tctcggcggc ggccgcggcg ctcgaacggg
     1861 tcatgcccgg cgtcgaggtg aatctctccg gcgtgaccgg ggggccgtcg gaggtcgtcg
     1921 accggcgcgg cgcgctgacc ggggcggccc ccggcgcggg cagcgagacg atcgtgcgcg
     1981 aattcctggg ccgtcacagc gccctcacgg gctctcggcc gccgacgtcg cggacctcgt
     2041 ggtcctcggc gacaggcccg gcggcacgag cggtctgcgc atgttgcgca tggagcagcg
     2101 gatcgacggc cgtcccgtct tccagagcga gacccgcttc ctcctcgacg gtgaaggccg
     2161 gctgatcaag agcgtcgggc tcacagcggc gaggtctggg cggtcaagct gtgggggctc
     2221 cgcaaccgga tcatcgccga tccggccggc gccaacggcg acgtgccgac cggcaaccag
     2281 acgaccctgc agctggtcat cgacggcctc aagatgacgc cggtcgatcc cgcgttcacc
     2341 gacggccggg acgccatcct cgatgccgac tgcgccacca acgcctgtgc ccacgaggac
     2401 ttcatctggc agacttcgcc gagcgcgggc tcggctgggg agcggcgcag ccgtgctggc
     2461 gaagcgaggc tcattcctcc tggggcagcc gttgccccgg cagaacggcc atcatccccg
     2521 ctggtatggc ctccgtaccc ggcgaaccag cctccgtacc cgaggtagga aggccgtacc
     2581 cacggggatg gccttcgcgc cctgggtatg gccttcgtac ccgacgggat agcttccgta
     2641 ccccgggtag ggaagcggta cctccgggga tggtctccct acccggggta tggcggtcaa
     2701 atcgccgggg aggacggccc tctccccggg tatggccctc gtttgtctct cccatctata
     2761 tagatgcagg ggatgggctt gtagctgcag ggggaagggc gtgcccctcc gggcgggggc
     2821 ttcctcccat aggcgctaac ttaaccctcc aagctgaaaa gcagcctgga cagcttgggg
     2881 ggcctgtttg ctgcgggctc ggggagagtc caagacgtga cgggcgatct cagaggcctt
     2941 gcggatgagc tcggtgaagc tcagct
//
LOCUS       DASPJI010000380         4858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_536581, whole genome shotgun sequence.
ACCESSION   DASPJI010000380 DASPJI010000000
VERSION     DASPJI010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536581"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VEL74_10105"
     CDS             complement(<1..273)
                     /locus_tag="VEL74_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62923.1"
                     /translation="MDPGLIGFAAIVSVPFALLVYAMGIVLRKEWRKTQEREKAAARP
                     GVERRLDRLLKRAEAQGLAMEEAALRLEEQVEELDQVREEHGRRLEN"
     gene            322..999
                     /locus_tag="VEL74_10110"
     CDS             322..999
                     /locus_tag="VEL74_10110"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HYG62924.1"
                     /translation="MRGVLVMMLGAVLVTVSGCSALVGVIDQATGADEARLLRQTGMP
                     AEAEVLRIWDTGTTVNDDPVIGMEVEVLPAEGEPFQAVIPRTWISRLDIPRFQPGRVI
                     AVRFDPETPARVALDDPPFPANDEEAGEPEGAAGVETSVGFGLRLCARTREGDGEGVE
                     TVFLVTGPAGRRFESQRIAPARGRWTCVLFPEDFAPPVPVPPGRYRYEIRVNGETAER
                     GVLTLDG"
     gene            complement(1112..1549)
                     /locus_tag="VEL74_10115"
     CDS             complement(1112..1549)
                     /locus_tag="VEL74_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62925.1"
                     /translation="MKMRTLLLVSLIILFTAGAALAQGSNMDCSCTAAGCGNGGVGGD
                     QGDIFINVPPAEVKQYFGPNTGWTCVVPSLVRGSGGPLGCFCAGYCGNGGIGADPNTF
                     DLGLSQDTINSSYGGGKPGNKTGWLCGDYRGSWNAAEHAPKKQ"
     gene            1835..2980
                     /locus_tag="VEL74_10120"
     CDS             1835..2980
                     /locus_tag="VEL74_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62926.1"
                     /translation="MRTLAWGLFLGLFLSIPVAAGVGAWTPEGGIEPVFSRLLADPGD
                     PETLYALVYMQDPDSSHALWKSTDGGESWRSIQNGIPPVHLLAVDPLQAGRLYAWDSS
                     NYSLPKLWASEDGGETWALRYDAEVRATVSQLWQILASPVRPGVLYGLSETVAGEPPF
                     GFAIYRSADGGATWTRRGAVGTSRASIESLLHHPARNRLEYFDNDGFYTSDDNGLTWM
                     VRGTFRGQGFQFVTRSHAAPDRLYGIPAMGAPCLVRSDDGGARWTRTRRPEFPGSLSC
                     TSLAVAPDDPGLLWVKAEGQLGSRYVHMISTSRDGGATWSRPRSFPSSFPIAVAGDPA
                     TLFAAGGDGYTAEGPFKSTDGGQTWTPSWDGIVTGDLRQALVALPSS"
     gene            3161..3922
                     /locus_tag="VEL74_10125"
     CDS             3161..3922
                     /locus_tag="VEL74_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62927.1"
                     /translation="MRSTDSGLSWGEATPGGPAFLGSLFADPFQSGRLFAATEESSTG
                     PTRVAVWRTADAGRSWARRHAGLPVTCTHIASVDVCPSLYAVTSDPRNPDRMLMSFGG
                     SGGLFSADPGVYVSPNGGRRWQLAAQSPPGIVISFAVDPTPSGTVFLAGTSKGIYRSA
                     DGGNHWSKLATSGLPVDAAVRQLLHDGQSGAWYAVTEGEGIFRSTDGGTTWAGINAGL
                     PDVARPVVVVDPQAPGRLFAAVRGQGLWSWSTPGS"
     gene            3965..>4858
                     /locus_tag="VEL74_10130"
     CDS             3965..>4858
                     /locus_tag="VEL74_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HYG62928.1"
                     /translation="MEPVEPSPESPSAPPPAPPSTTPSDLREQPPAGGSWRADALARS
                     GHSRLGEEIRPPQEDLDEDRSHEVCATICRNLLGDFTPALLLLSAAERRRAQAFLAYT
                     FTLFDFARQRGVEGERLAQINRWEFTLEAALSGQPVGQPVFVGMAREQKRRPWPPEAL
                     DAVATCARRRATRRRPATPEEADAEAERLARAVGHGLIGAPPPGEVSAFAGALVRLRT
                     LQVLGSEMRGRRCPIAVSELPDGDDEPGPEPARLLAAAQRECRRLRPRLLRASRGLVD
                     LPPGYRRGAIFCLLAGLHLLTA"
BASE COUNT          647 a         1793 c         1680 g          738 t
ORIGIN      
        1 gttctcgaga cgcctgccgt gttcttcacg gacctggtcc agctcttcga cctgctcttc
       61 caggcgcagc gcggcctctt ccatggcgag cccctgcgcc tcggctcgtt tcagcaaccg
      121 gtcgagccgc cgttccacac cgggcctcgc cgcggccttt tccctctcct gggtcttgcg
      181 ccactccttg cgcaggacga tacccatcgc atacaccagg agggcgaagg gtacggagac
      241 gatggcggcg aaaccgatca gtccgggatc catggcggcc cccctagtct atccttctcc
      301 catctggaca ggaggtagcc gatgcgcggc gtgctcgtga tgatgctggg ggcggtgctg
      361 gtgacggtgt ctgggtgctc tgcgctggtc ggggtgatcg atcaggcgac cggcgcggac
      421 gaggcccggc tgctgcggca gaccggcatg ccggccgagg ccgaggtcct gcggatctgg
      481 gacaccggga cgacggtcaa cgacgatccg gtgatcggga tggaggtcga ggtgctcccc
      541 gcggagggcg agccgttcca ggccgtcatc ccgcggacct ggatctcgcg cctcgacatc
      601 ccgcggttcc agcccggccg ggtcatcgcc gtccgcttcg accccgagac tccggcccgc
      661 gtggcgctcg acgatccccc cttcccggcc aacgacgagg aagccggcga gccggagggc
      721 gccgccggcg tcgagaccag cgtgggcttc ggcctgcggc tgtgcgccag gacccgggag
      781 ggggacggcg aaggcgtcga gaccgtgttc ctcgtcaccg gaccggcggg ccggcgcttc
      841 gagtcccagc ggatcgcccc ggcgcgcggc cggtggacct gcgtcctctt tcccgaggac
      901 ttcgccccgc cggtgcccgt ccccccgggc cgctaccgct acgagatccg cgtcaacggc
      961 gagaccgccg agcgcggcgt cctcaccctt gacggctgac ggcggaacgg tgagacgacg
     1021 cggccggcgc gcagaggttc ccggcctgat ccaaaaggaa gcgccggccc ggaagcccgc
     1081 ggccggcgct cctacaccct ccggaccggt gctactgctt cttgggcgcg tgctcggccg
     1141 cgttccacga gccgcgatag tcgccgcaca gccagccggt cttgttgccc ggcttgccgc
     1201 cgccgtagct cgagttgatg gtgtcctggc tcaggcccag gtcgaaggtg ttggggtcgg
     1261 cgccgatgcc accgttgccg caataaccgg cgcagaagca ccccagcgga ccgccggagc
     1321 cacggaccag cgacggcacg acgcaggtcc agccggtgtt ggggccgaag tactgtttca
     1381 cctcggcggg cgggacgttg atgaagatgt ccccctggtc gccaccgacg ccgccgttgc
     1441 cgcagcccgc ggcggtgcac gagcagtcca tgttggagcc ctgggcgagg gccgcgccgg
     1501 cggtgaacag gatgatgaga ctgacgagaa gcagggtacg cattttcatg acagacctcc
     1561 tccttggggt ttcgttttca ctccctcgaa tgcgcttgcg cgttcgcttc gaaccaggat
     1621 aaaggcaacc tttgtgccag cctggcgaac gctgaaacga tgagcggaaa tcccttttag
     1681 atcagtcatt tggccggacc tgagcgcccc tggcagccga ttgtcgacgg atgacgctct
     1741 atgtcggcca atgtcgatcg acatgtcggc tcgcctcctc tccttgactt ccattcgctc
     1801 ccggacagac gatggaggga gaggagatcc attcatgaga actctcgcct gggggttgtt
     1861 ccttggcctt tttctgtcga ttcccgtcgc ggcgggcgtg ggggcttgga cgccggaggg
     1921 agggatcgag ccggtctttt cgcggctgct ggcggacccc ggagacccgg agacgctcta
     1981 cgcgctggtc tacatgcagg accccgacag ctcccacgcg ctctggaaga gcaccgacgg
     2041 cggggagagc tggagatcca tccagaacgg catccccccg gtccacctgc tggcggtcga
     2101 tccgctgcag gccgggcggc tctacgcctg ggacagctcc aactactcgc tgccgaagct
     2161 ctgggcgagc gaggacggcg gcgagacctg ggcgctccgc tacgacgccg aggtccgcgc
     2221 gacggtctcc cagctctggc agatcctcgc cagcccggtc cgccccggag tcctctatgg
     2281 cttgagcgag acggtggccg gcgagccgcc gttcggcttc gccatctacc ggtcggccga
     2341 cggcggcgcg acgtggacgc gccgcggcgc ggtgggcacc agccgcgcct ccatcgagtc
     2401 gctcctccac catccggcgc gcaaccggct cgagtatttc gacaacgacg ggttctacac
     2461 cagcgacgac aacggcctca cctggatggt gcgggggacc ttccgcggcc aggggttcca
     2521 gttcgtgacg cggagccacg cggccccgga ccggctctac ggcatcccgg ccatgggcgc
     2581 gccgtgcctg gtccgcagcg atgacggcgg cgctcgctgg acgaggacgc gccggcccga
     2641 gttcccgggc agcctgtcct gcacctccct tgccgtcgcg ccggacgatc ccggccttct
     2701 atgggtgaag gccgaaggac agctcggcag ccggtacgtc catatgatct cgacctcgcg
     2761 ggacggcggc gccacctggt cgcgtccccg gtccttcccc tcctccttcc cgatcgcggt
     2821 ggcgggcgat cccgcgacgc tcttcgcggc cggcggcgac ggctacacgg ccgaaggtcc
     2881 tttcaagagc accgacggcg ggcagacctg gacaccgtcc tgggacggca tcgtgaccgg
     2941 cgacctgcgc caggccctcg tcgccctccc ctcgtcctga ccgaaccctc tcctcgtctc
     3001 cgtcgtccgc accgcaggct ccgcgggcgg ccggatcgct cgcagcctgg acggcggcgc
     3061 cacctgggag ctgcccgcgc tcccccgcga gtactggaac cttgccgccg acgccaacgg
     3121 ccggctcctc tatgcgctcc ggtccgacac cggccgggtg atgaggagca ccgacagcgg
     3181 cctgagctgg ggcgaggcga cgcccggtgg cccggccttc ctgggatctc tcttcgccga
     3241 tccgttccag tcgggacggc tcttcgccgc caccgaggag agctcaaccg ggccgacgcg
     3301 ggtcgccgtc tggcgcaccg ccgacgcagg gaggagctgg gcgcgccgcc acgccggtct
     3361 cccggtcacc tgcacgcaca tcgcctcggt cgacgtctgc ccctccttgt acgcggtgac
     3421 ctccgatcca cggaaccccg accggatgct gatgtccttc ggtggatccg gcggcctctt
     3481 ctccgccgat ccgggggtct atgtcagccc caacggaggg cggcgctggc agctcgcggc
     3541 tcagagcccg cccgggatcg tcatctcctt tgccgtggac cccaccccct ccggcaccgt
     3601 cttcctggcc ggaacgtcga agggcatcta ccgatcggcg gacgggggga accactggtc
     3661 gaagctcgcg accagcggcc tgccggtgga cgcggcggtc cggcagctcc tacacgacgg
     3721 ccagagcggc gcctggtacg ccgtcaccga aggcgaaggc atcttccgca gcaccgacgg
     3781 cgggaccacg tgggccggca tcaacgccgg cctgccggat gtcgccaggc cggtcgtcgt
     3841 ggtcgatccc caggccccgg gccggctctt cgccgcggtc cgcggccagg gcctgtggag
     3901 ttggagcacg ccgggctcct gaatcgcctg acacgggctc catccctctg ctacgctggg
     3961 agggatggag cctgtcgagc cctctccgga atccccgtcc gctccgccgc ccgcgcctcc
     4021 ctccaccacg ccatccgacc tccgcgagca gccgcccgcg ggggggagct ggcgggcgga
     4081 cgcgctggcg cgcagcggcc acagccgcct cggggaggag atccgaccgc cgcaggagga
     4141 tctggacgag gaccggtcgc acgaggtctg cgccaccatc tgccgcaacc tgctggggga
     4201 cttcaccccg gccctgctcc tgctctcggc ggccgagcgc cggcgggcgc aggcgttcct
     4261 cgcctatacc ttcaccctgt tcgacttcgc ccgccagcgc ggcgtcgagg gggagcgcct
     4321 ggcgcagatc aaccgctggg agttcacgtt ggaggcggcg ctttcggggc agccggtggg
     4381 gcagccggtg ttcgtaggca tggcgcgcga gcagaagcgg cgcccctggc cgccggaggc
     4441 cctggacgcg gtggccacct gcgcccgccg gcgcgccacc cgccgccgcc ccgcgacccc
     4501 cgaggaggcg gacgccgagg ccgagcgcct ggcgcgggcc gtgggtcacg gcctcatcgg
     4561 cgcgccgccg ccgggcgagg tgagcgcctt cgcgggcgcc ctggtgcggc tgcgcacgct
     4621 gcaggtcctg ggctccgaga tgcgcggccg gcgctgtccc atcgccgtct ccgagctgcc
     4681 cgacggcgac gacgagccgg gacccgagcc ggcgcgcctc ctcgcggccg cccaacggga
     4741 gtgccggcgc ctgcgccccc ggctgctacg ggcctcgcgg ggactggtgg acctgccgcc
     4801 gggctatcgg cgaggcgcca tcttctgtct gctggcgggc ctccacctcc tcaccgcg
//
LOCUS       DASPJI010000381         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_832455, whole genome shotgun sequence.
ACCESSION   DASPJI010000381 DASPJI010000000
VERSION     DASPJI010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832455"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VEL74_10135"
     CDS             <1..628
                     /locus_tag="VEL74_10135"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYG62929.1"
                     /translation="YLDRQVGRLLDFLESRGALDDTLVVLVADHGESLGEHGIVFRHV
                     GLHDTTTHVPMMIRWPGPGKKGRRIEGLVQTIDLFPTILGAVGLPVPQQDGEDLRKLT
                     GDGRKGRRAVFAEHAAKLGLMARTREHKYILSQGNPQFFPDGPSLYDVNADPGETRNL
                     AGQNLAAERQMADLLFRWLADRRHHTETTPKDLSPEEIERLRALGYIR"
     gene            complement(603..1358)
                     /gene="tatC"
                     /locus_tag="VEL74_10140"
     CDS             complement(603..1358)
                     /gene="tatC"
                     /locus_tag="VEL74_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HYG62930.1"
                     /translation="MSLLDHLEELRSRLVWALLTVAIAFAPCWYFVDRIFDFLQQPIL
                     AELEPGQRLVYTGIADPLFLYIKVAALAAVFLSAPFLLFQVWRFVAPGLYRREKLFVL
                     PFLIFGTLFFVGGGAFAYYIAFPKAVDFLLGMAAQFQPMIEVDRYFGFLMTIILGMGL
                     MFELPILVVALSAMGVVTPRFLLRNFRWAVLIVFTVSAIITPTPDILNLSLFAVPALV
                     LYLLGIGVAWFVSPRQRAEEADESTPTGYTPAP"
     gene            complement(1416..1841)
                     /gene="tatA"
                     /locus_tag="VEL74_10145"
     CDS             complement(1416..1841)
                     /gene="tatA"
                     /locus_tag="VEL74_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009350244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HYG62931.1"
                     /translation="MFGPLGVPELIFILVLALLIFGPKRLPEIGRTLGKGMSEFRKAS
                     NDLKRTINTELALEETPAPPSSPSSSVSPGTTRRLPAMEAMDQPPAEPEPPAFRMPDG
                     PPAEVEARSPARPLPPEWTEPPEPQSAANASTAEPLEPR"
     gene            1938..>2652
                     /locus_tag="VEL74_10150"
     CDS             1938..>2652
                     /locus_tag="VEL74_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HYG62932.1"
                     /translation="MRRPWRIAAITAFTLALILGGLLGNRLLALTDEARDSLRLYTEL
                     VTVAHERYGADVRYSDLVYSSVDGMVRTLDPHTSFLPPDIYQGMREKQQTTYYGLGLL
                     VGFRNGQLTVISPMEGTPGARLGVMAGDVISTIDGEPTETLSLDEAVDRLKGEKGTQV
                     RITILRRGLERPLEMTVTRAEIPQTTVRQAYMLTPAAGYISISEFNRSTGREVAEAVA
                     KLKQQGMRQLLIDLRNNGGG"
BASE COUNT          472 a          909 c          924 g          347 t
ORIGIN      
        1 ctacctcgac cgccaggtcg gacgcctgct cgacttcctg gagagccggg gggcactgga
       61 cgacaccctg gtggtgctcg tggcggacca cggcgagagc ctgggcgagc acggcatcgt
      121 cttccggcac gtcggtctcc acgacaccac cacccacgtg ccgatgatga tccgctggcc
      181 cggcccgggg aagaaggggc ggcggatcga ggggctggtg cagaccatcg acctcttccc
      241 caccattctg ggcgcggtgg gtctgccggt gccgcagcag gacggcgaag atctgcggaa
      301 gctgaccggc gacggccgca aggggcggcg ggcggtcttc gcggagcacg cggccaagct
      361 cggcctgatg gcgcgcaccc gcgagcacaa atacatcctg agccagggca atccccagtt
      421 cttccccgac ggcccctccc tctatgacgt gaacgccgac cccggcgaga cccgcaacct
      481 cgccggccag aacctcgcgg ccgagcggca gatggccgac ctcctcttcc gttggctcgc
      541 cgaccggcgg caccacacgg agaccacgcc caaggacctg tcgccggaag agatcgagcg
      601 cctcagggcg ctggggtata tccggtaggg gtcgactcgt ccgcctcctc cgcccgctga
      661 cgggggctga cgaaccaggc gacgccgatg cccaggaggt agaggaccag ggccggcacg
      721 gcgaacaggg agaggttcag gatgtccggc gtcggcgtga tgatcgccga cacggtgaag
      781 acgatcagca ccgcccaccg gaaattgcgc agcaggaagc gcggcgtcac cacccccatg
      841 gccgacagcg caaccaccag gatcggcagc tcgaacatca gccccattcc caggatgatg
      901 gtcatcagga agccgaagta gcggtccacc tcgatcatcg gctggaactg cgccgccatc
      961 ccgagcagga agtcgaccgc cttggggaag gcgatgtagt aggcgaaggc gccgcccccg
     1021 acgaagaaga gcgtgccgaa gatcaggaac ggcaggacga agagcttctc gcgccggtag
     1081 agccccggtg ccacgaagcg ccagacctgg aagaggagga agggggcgga gaggaagacc
     1141 gccgccagcg ccgccacctt gatatagagg aagagcgggt cggcgatccc ggtgtagacc
     1201 aggcgctgcc ctggctccag ctcggcgagg atcggctgct gcaggaagtc gaagatccgg
     1261 tcgacgaagt accagcaggg cgcgaaggcg atggcgacgg tcagcagcgc ccagacgagg
     1321 cgcgagcgca gctcctcgag gtggtcgagg agcgacatgc gctccaggtc ttcggggggg
     1381 aggctcatga ggagagggag ggcggagccc cggacctagc ggggctccag cggttccgcg
     1441 gtgctcgcgt tggccgcaga ctggggctcc ggcggctccg tccattccgg cggcaggggc
     1501 cgcgccggcg acctggcctc gacctcggcc ggcgggccgt cgggcatccg gaacgccggc
     1561 ggctcgggct cggcgggcgg ctggtccatc gcctccatcg ccggcaaccg gcgcgtcgtc
     1621 cccggagaca cggacgatga cggcgacgag ggcggcgcgg gcgtctcctc gagggccagc
     1681 tccgtgttga tggtgcgctt gaggtcgttg gaggccttgc ggaactccga catccctttg
     1741 cccagggtgc ggccgatctc cggcagccgc ttggggccga agatgaggag cgccaggacc
     1801 aggatgaaaa tcagctcggg tacgcccagg ggaccgaaca tcgagaagcc tccaggctcg
     1861 gatcatcggg ccacgccgcc gccaaagtca agagcgcgcg ggaagaccgc cccgccccct
     1921 ggtgttacac ttctccaatg agacgtccat ggcgtattgc agcgatcacg gccttcactc
     1981 tcgcgctgat cctgggtggc cttctcggca accggctcct cgccctgacc gatgaggccc
     2041 gggacagcct ccggctctat accgagctgg tcaccgtggc tcacgagcgc tatggcgccg
     2101 atgtgcgcta cagcgacctc gtctactcct ccgtcgacgg catggtgcgg accctggatc
     2161 cgcacaccag cttcctcccg cccgacatct accagggcat gcgggagaag cagcagacca
     2221 cctactatgg gctgggcctg ctggtgggct tccgcaacgg gcagctcacc gtcatctccc
     2281 ccatggaggg gacccccgga gcgcggctcg gcgtcatggc gggcgacgtc atcagcacga
     2341 tcgacggcga gcccaccgag accctgtccc tcgacgaggc ggtcgaccgc ctcaaggggg
     2401 agaaggggac ccaggtccgc atcaccatcc tgcggcgcgg cctcgagcgc ccgctggaga
     2461 tgacggtgac ccgcgccgag atcccccaga ccacggtccg tcaggcctac atgctgaccc
     2521 ccgccgcggg ctacatctcg atctccgagt tcaaccgctc caccggccgc gaggtggcgg
     2581 aggcggtggc caagctcaag cagcagggga tgcggcagct cctcatcgac ctgcgcaaca
     2641 acggcggcgg cg
//
LOCUS       DASPJI010000382         4648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_657188, whole genome shotgun sequence.
ACCESSION   DASPJI010000382 DASPJI010000000
VERSION     DASPJI010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657188"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4648)
                     /locus_tag="VEL74_10155"
     CDS             complement(<1..>4648)
                     /locus_tag="VEL74_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG62933.1"
                     /translation="ALGRPIAHTRAYLLDRAGLPVPLGVAGELCAAGDGLARGYLGRP
                     DSTAQAFIPDPFGAPGGRLYRTGDLARHLPDGRLEFLGRLDQQVKLRGFRIEPGEIEA
                     ALLLHPRVREAAILVREDIPGDRRLVAYVAADGIEAGDLRQRLQQRLPDYMVPSAFVV
                     LDALPLNQNGKVDRRALPAPARELPAAAAALRTPTEELLAAIFATVFGVEEVGLEESF
                     FDLGGHSLLATRVISRVRETFGADLSLSVLFDRPTVAGLAAAMESEQSAGRLAAAPPL
                     VPVEWDRPLPLSFAQERLWFLDRLQPGSAVYNMPAALRLDAGVDAAALARALGAVVSR
                     HEALRTTFHLAADGPVQRIAEPPSAAGILARIDLAGLPVARRRDEALRLATAEAARPF
                     DLAAGPLLRAALLHLGDGGALLLLTLHHIVADGWSMDLLADEVTAHYQAALSGGAAPM
                     PELPIQYADFAVWQRQWLSGEELERQLAHWRERLAGAPTVLELPTDRPWLALPDSRGA
                     ERRRVLPAGLARDLRALGRAQGTTLFMTLLAAFGALLSRYSGQEDLVLGTPVAGRNRV
                     EVEPLIGLFVNTLALRVDLSGSPSFRELAHRARETALAAYAHQDLPFEKLVEELRVER
                     DLGRSPVFQAMLALPVTEHSADAADAAEVDETDWAAGTGTAKFELALEARETAAGLAL
                     EAEYRTDLFDGVTIERLLGHLELLLRQVVADPKRPLVDAALLAGAERHALLAEWNDTA
                     SPGTMPALCVQELFARRAALAPEATALVMAAKRLSYGELEGRANQLARHLRRLGVGPG
                     VPVGVCLERSLEMGEAILGVLKAGGCYVPLDPAYPRERLAFMIEDAGLDVLLTQDALA
                     GLLPGARRLLRVDADAHSWAGESDADPGVEVHPESLAYVIYTSGSAGRPKGVQVPHRA
                     LANHATAVAERYGLTPADRVLQFASLSFDVAAEELFPAWISGAALVPRPAGLFPSFQE
                     LEHLLSHQRITVANLPTPYWHEWVDDLARTGTAPPASLRLLVVGTEQAAPERLTAWRR
                     LAGDHVRWINAYGPTEATITATLHEAGATAAAAPAARVPIGRPIARLQAHVLDAAFEP
                     APLGVVGELYLGGAGVARGYLHHPDRTAAVFLPDPFSGLAGARLYRTGDRARRFPSGE
                     LDFLGRADDQVKIRGFRIEPGEIEAALRRLPEVQDCCVVVREDAGERRLVAYAVPGAA
                     AARGGSLIDALRASLAASLPSYMMPSAFVLLEALPLTPNGKVDRQALSAAAAPAAAAP
                     PEVTAPRTPVEERLAGIFAAVLGVERVGVEESFFELGGHSLLATRVMSRVSEALGLDL
                     PLRTLFEAPSVARLAAVVEAAGGASLQALPPLEPVRRDQPLPLSFAQERLWFLAQLEP
                     ESAAYNMPQALPLEAGLDPWLLERALADVARRHEALRTTFPPSSGQPVQRIAPPAAAW
                     SLPRVDLAALPAVARAAEASRLAGLEAGRPFDLTAGPLLRTTLLQLGAPGQWLLFTLH
                     HIIADGWSLDLLEEEVTALVQAFARGEASPLPELPVQYADFAVWQRRTLTEDRLA"
BASE COUNT          686 a         1672 c         1772 g          518 t
ORIGIN      
        1 gcgagccgat cctcggtcag ggtgcggcgc tgccagacgg cgaagtcggc gtactggacc
       61 ggcagctccg gcagcggcga ggcctcgccc cgcgcgaacg cctggaccaa ggcggtcacc
      121 tcctcctcca gcaggtccag agaccagccg tccgcgatga tgtggtggag ggtgaagagc
      181 agccattggc ccggcgcgcc caactgaagc agggtggtcc gcagcagcgg gccggcggtc
      241 aggtcgaagg gacggccagc ctcgaggccg gcgaggcgtg aggcttcggc ggcccgcgcc
      301 accgccggca gagccgcgag atccacccgg ggcaggctcc aggcggcggc aggtggagcg
      361 atccgttgca ccggctgacc actggacggc gggaaggtgg tgcgcagcgc ctcgtggcgg
      421 cgggccacgt ccgccagcgc gcgctccagc agccacgggt cgaggccggc ctccagaggc
      481 aaggcctgtg gcatgttgta ggcggcgctc tcgggctcga gctgggcgag gaaccagagc
      541 cgctcctggg cgaaggagag agggagcggc tggtcgcggc gcaccggctc cagggggggg
      601 agggcctgga gggaagctcc tccggccgcc tccaccacgg cagccagccg ggccacggag
      661 ggggcttcga agagggtccg cagcggcagg tccagcccga gcgcttcgct cacccgcgac
      721 atcacccggg tcgccagcaa cgagtggccg cccagctcga agaagctctc ctccacccct
      781 acccgttcga ctccgagcac ggcggcgaag atcccggcca gccgctcctc gaccggcgtg
      841 cgcggcgccg tgacctccgg cggcgccgcc gctgccggcg ccgcggcggc ggacaacgct
      901 tggcggtcca ccttgccgtt gggggtgagc ggcagggcct ccaggagaac gaaggccgac
      961 ggcatcatgt aggacggcag ggatgcggcc agggatgccc gcaacgcgtc gatcagagag
     1021 ccgccccggg cggccgcggc gccggggacg gcgtaggcca ccagacgcct ctcccccgcg
     1081 tcctcgcgca ccacgacaca acagtcctgg acctcgggca ggcggcgcag agccgcctcg
     1141 atctctcccg gctcgatacg gaaaccgcgg atcttcacct gatcgtcggc gcgccccagg
     1201 aagtccagct cgccggaggg gaaacggcgc gcccggtcgc cggtgcggta gaggcgggct
     1261 ccagcaaggc ccgagaaggg atcgggcagg aagaccgccg cggtacggtc gggatggtgc
     1321 aaatagccgc gggcgacgcc ggcgccgccc aggtagagct cgcccaccac ccccaggggg
     1381 gcgggctcga acgcggcgtc cagcacgtga gcctggagcc gggcgatggg tcggccgatg
     1441 gggacccgcg ccgccggggc cgcggcggcc gtcgcgccgg cctcgtggag tgtggctgtg
     1501 atcgtggcct cggtgggacc gtaggcgttg atccagcgga cgtggtcccc ggccagccgg
     1561 cgccaggccg tgagacgctc gggcgcggcc tgttcggtgc ccaccaccag gaggcggagc
     1621 gaggccggag gagccgtgcc ggtgcgggcg aggtcgtcca cccactcgtg ccagtagggc
     1681 gtggggaggt tcgccacggt gatccgctgg tgagagagca ggtgctccag ctcctggaac
     1741 gaagggaaga ggccggcggg ccgcggtacc agagcggcgc ccgagatcca ggccgggaac
     1801 agctcctcgg cggcgacgtc gaaggagagc gaggcgaact gcaggacgcg gtcggccggc
     1861 gtcagcccgt accgctcggc caccgcggtg gcgtggttgg cgagggcccg gtggggcacc
     1921 tgcaccccct tgggacggcc ggccgagccc gaggtataga tcacgtacgc cagcgactcg
     1981 gggtggacct ctacccccgg atcggcgtcg ctctcgccgg cccacgagtg ggcgtcggca
     2041 tcgacgcgca ggagccgccg cgcgccgggc agaagaccgg ccagcgcgtc ctgggtcagc
     2101 aggacgtcca ggccggcatc ctcgatcatg aaggccaggc gctcccgtgg gtaggccggg
     2161 tccaggggaa cgtagcagcc gccggccttg agcacgccga ggatcgcctc ccccatctcc
     2221 agcgaccgtt ccaggcagac gccgaccggt acgccgggtc ccacacccag gcgccggagg
     2281 tggcgcgcca gctggttggc ccggccctcg agctcgccgt agctcagacg cttcgccgcc
     2341 atcaccagcg ccgtggcttc cggcgccagc gccgcccggc gcgcgaacag ctcttgaacg
     2401 cagagcgccg gcatcgtgcc gggcgaggcc gtgtcgttcc attccgccag caacgcgtgg
     2461 cgctcggcgc cggcgagcag cgcggcatcc accagcgggc gcttggggtc ggccaccacc
     2521 tgccgcagca gcagctccag gtggccgagc aggcgttcga tggtgacgcc gtcgaacagg
     2581 tcggtcctgt actcggcttc gagggcgagg ccggcggccg tctccctggc ctccagcgcg
     2641 agctcgaact tggccgtccc cgtgcctgcc gcccagtccg tctcgtccac ctcagcggca
     2701 tcggcggcat cggcggaatg ctccgtcacc ggcagcgcca gcatcgcctg gaacaccggc
     2761 gaccggccga gatcccgctc gacccgcagc tcctccacca gcttctcgaa cggcaggtcc
     2821 tggtgggcgt aggccgcgag ggcggtctcc cgggcccggt gcgccagctc gcggaaggag
     2881 ggagagccgg acagatccac ccgcagcgct agggtattga cgaagaggcc gatgagcggc
     2941 tccacctcca ctcggttgcg gccggccacc ggcgtgccca ggacgaggtc ctcctgacca
     3001 gagtagcggc tcagcagcgc gccgaacgcc gccagcaggg tcatgaagag ggtcgtcccc
     3061 tgagcgcggc ccagagcccg aagatcacgc gcgaggccgg ccgggagcac ccgccgccgc
     3121 tcggctcccc gggagtccgg cagcgcgagc cacggccggt cggtgggcag ctccaggacc
     3181 gtgggcgcgc cggccagccg ctcccgccag tgggcgagct ggcgctccag ctcctcgccc
     3241 gacagccact gccgctgcca gacggcgaag tccgcgtact ggatcggcag ctcgggcatc
     3301 ggcgcggccc cgccggagag tgcggcctgg tagtgggccg tcacctcgtc cgccagcagg
     3361 tccatggacc agccgtcggc gacgatgtga tgcagggtga ggagcagcaa cgctccacca
     3421 tcgcccagat gcagcagcgc cgcgcgcagc agcggcccgg cggccaggtc gaagggacgc
     3481 gccgcctccg ccgtggccag ccgcagcgct tcgtcgcggc gccgggccac cggcaggccg
     3541 gcgaggtcga tccgggccag gatgccggcg gcggaggggg gctccgcgat ccgctgcacc
     3601 ggcccgtccg cggccagatg aaaggtggta cggagcgctt cgtgccggct caccaccgcg
     3661 ccgagcgccc gcgccagggc cgcggcgtcg accccggcgt ccaggcggag cgcggcgggc
     3721 atgttgtaga cggcgcttcc cggctggagg cggtcgagga accagaggcg ctcctgggcg
     3781 aaggagagcg gcagcggccg gtcccattcc accggcacca gggggggcgc cgcggccagc
     3841 cgtcccgccg actgctcgga ctccatcgcc gcggccagac ccgccacggt cggccgatcg
     3901 aacagaaccg acagcgacag gtcggcgccg aacgtctccc gcacccgcga gatcacccgg
     3961 gtggcgagga gcgagtgccc gcccagatcg aagaagctct cctccaggcc gacctcctcg
     4021 acgccgaaga cggtggcgaa gatcgccgcc aacagctctt cggtgggagt acggagggcg
     4081 gcggccgccg ccggcagctc tctggcaggc gcgggcagcg cccggcggtc caccttgccg
     4141 ttctggttca ggggcagagc gtcgaggacg acgaaagccg aggggaccat gtagtccggc
     4201 aggcgttgct gcagccgttg ccgcaggtcg ccggcctcga tgccgtccgc ggccacatag
     4261 gccaccagcc gccggtcgcc ggggatgtct tcgcgcacca ggatggccgc ctcccgcacg
     4321 cgcgggtgaa ggagcagcgc ggcctcgatc tcgccgggct cgatgcggaa gccgcgcagc
     4381 ttgacctgct gatcgagccg ccccaggaac tcgagccggc cgtcgggcag gtggcgggcc
     4441 aggtctcccg tgcggtagag gcggccgccc ggggcgccga agggatcggg gatgaaggcc
     4501 tgggcggtcg agtccggccg gccgaggtag ccgcgcgcca ggccgtcgcc ggcggcgcac
     4561 agctctccag ccacgccgag cgggaccggc agtccggcac ggtccaggag gtaggcccgg
     4621 gtgtgggcga tgggccggcc gagtgcca
//
LOCUS       DASPJI010000383         6286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_138772, whole genome shotgun sequence.
ACCESSION   DASPJI010000383 DASPJI010000000
VERSION     DASPJI010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138772"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..789)
                     /locus_tag="VEL74_10160"
     CDS             complement(85..789)
                     /locus_tag="VEL74_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HYG62934.1"
                     /translation="MSTASAAPPLPVLYQDDALIAVAKPSGLAVHRGWAEDRVVLMTL
                     VRDLLGRQVHPVHRLDRGTSGVVLLAFDPEITRRLQEQFEAGRVEKRYLALVRGIPPE
                     EGVIDNPVPRAPDGPRVPAVTEYRRLGTFERYALVEAFPRTGRLHQIRRHFKHISHHL
                     IGDVRYGKGEHNRLFRERFGLHRLALHALELAFDHPVDGRPLRLFAPVPEDLAGPLRA
                     MGLPVPVSRYPAEAAP"
     gene            complement(818..1441)
                     /locus_tag="VEL74_10165"
     CDS             complement(818..1441)
                     /locus_tag="VEL74_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HYG62935.1"
                     /translation="MAEAEDGSKRFEILCGVVIALIAAVLAVTDLGAGKYGDDELIAH
                     NEKNGAFLWYQSKGIKESLVEGQRDTLKALVEAGSIQPAQLTGVQSQIAHLEQEAERY
                     DKEKKEILLGSKTVGQANWVQDVDGEMGKVTGAQEWESLGEGLGAAGDTFDYSTFFLQ
                     LSLVIGAISLILQHDRMRTIFFTIMVVLGATGTLYSVLAFLQAGRVG"
     gene            complement(1571..2257)
                     /locus_tag="VEL74_10170"
     CDS             complement(1571..2257)
                     /locus_tag="VEL74_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011134897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HYG62936.1"
                     /translation="MEALKDLIDIFLHLDKHLKPILETYGAWTYGLLFAIVFCETGLV
                     VTPFLPGDSLLFAAGTFAATGSLSLWWLMLLLFIAAVLGDTVNYWIGHSLGPRLLKMR
                     RIIKPEHLQRTHDFYERYGGKTIVIARFVPIVRTFAPFVAGLGSMTYGRFMTYNVAGA
                     ALWVVLCTMAGYLFGNLPFVQENFSLVILGIIFVSILPAVIEILRARGAKRRGDAAAA
                     ARDAANLPTD"
     gene            2439..4211
                     /locus_tag="VEL74_10175"
     CDS             2439..4211
                     /locus_tag="VEL74_10175"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HYG62937.1"
                     /translation="MTLSIFIVLALLVAVVVVFAFEWVSVDVATLLLLSALVLTGILS
                     VEEAFSGFSNDIIIILASIFILSGALMKGGVMDRLGDAIHRVAGGSRTKVMLGVLPVT
                     TIVSSFMNNTTTTAILMPATLGLCRKSKLSASKVLIPLAYASMLGGTCTLIGTSTNVA
                     ASGYVASSGLEPFSMFEFLPAGLAICLLGIGYLILVGHRLLPDVRETGYLEEYAIREF
                     LSEVVITPGSPLSGQAIRDSRLAQMDLQVRAVHRGERRLDPEPGLRLEEGDLLIVQTS
                     REGLLQIKETAGIEIKPDITLAAEDLTSETMTIGEAILMPGSSLAGRSVKELDFRRRF
                     GVIVIAIYRRGHTLATRLGTLPLRVGDVLLLQGRPDRFRALADNSDLWILQEVEHRPA
                     RRRKGFYAMGIFLLAVAASAFELVPLPIAFLLAALGVIGTRLITMEEAYDLIDWRLLI
                     LIAGMTSFGIAMRKTGAAEFIAGLVVQWVSPLGVTAVMLAFVILTMLLTQPMSNAAAA
                     LVVLPVAMHTATSLGLDPRTFAVLVTLAASLSFITPFEPSCLIVYGPGKYRFRDFVTC
                     GLPLTALVVVSLILLVPMIWPLRQ"
     gene            complement(4219..5298)
                     /gene="mnmA"
                     /locus_tag="VEL74_10180"
     CDS             complement(4219..5298)
                     /gene="mnmA"
                     /locus_tag="VEL74_10180"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HYG62938.1"
                     /translation="MSGGLDSSVTAWLLSKGDRPVIGLSMLLWDHSGVEAHGRCCGAL
                     DLGDAKRVAQQAGIPHYTLRMDEDFRRQVVDPFVQDYLEGRTPSPCVRCNTWIKFDLL
                     LERARRLGAERVATGHYARILDGPGGPELHTAVDAGKDQSYYLFELTQEQLAASLFPL
                     GAMTKGEVRALAREAGLAVAGKGESMEVCFVSGGVREFVEAQVAEHPERFGTVAEPAP
                     STLVDTAGRELGTGQPYYRYTVGQRRGLGLAARSPLYVLAIEPEENRVVVGEEEALLA
                     PGLQGERLHWIGADPSLGEAGEVEATVKIRSRHPGVAARIRPLDKGEVEVRFAEPQRG
                     VTPGQAAVFYQGTRVLGGCWIRGRL"
     gene            complement(5331..>6286)
                     /locus_tag="VEL74_10185"
     CDS             complement(5331..>6286)
                     /locus_tag="VEL74_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HYG62939.1"
                     /translation="SSGTEANNAVLFGISSRADGPGHLVISAFEHPSVREAAARLEKS
                     GWSVTRVGPGPDGVVPAAAVVSALRPDTRLVCLMLANNELGTVQPVAEVAAACRARGI
                     PVLCDAVQAVGKIPVSAEALGADYLTLGAHKFHGPPGVAALWVRKGVELPGYLVGGSQ
                     ERRRRASTENVPGIVGLGEAAARTAAELPERAARLAALRDRFEAGLRAIPDAAVHCQG
                     SPRLPNTSHVAFHGVEGESLLIRLDLAGFAVSTGSACSSGAVEPSKTLLAIGLSPEEA
                     LSSLRVSVGMTNRPEEIDAFLAALAREVAALRRVAPLEVAR"
BASE COUNT          900 a         2241 c         2218 g          927 t
ORIGIN      
        1 ccactcccgt gcgggggacg gcgcggaagg cctggagcga gccgctgcgg agggcggcgg
       61 gggaagacat cacgaccaca tcgttcatgg tgcggcctcc gccggatacc gagacaccgg
      121 caccggcagc cccatggcgc gcaacgggcc ggccaggtcc tcggggaccg gggcgaagag
      181 ccgcaggggg cggccgtcca ccgggtggtc gaaggcgagc tccagggcgt ggagggcgag
      241 ccggtggagc ccgaagcgct cccggaacag ccggttgtgc tcccccttgc cgtagcgcac
      301 gtcaccgatg aggtggtggc tgatgtgctt gaagtgccgg cggatctggt ggagccgccc
      361 ggtccggggg aacgcctcca ccagggcgta gcgctcgaag gtgccgagcc gccggtactc
      421 ggtgaccgcc ggcacccgcg gcccgtccgg cgcccggggg accggattgt cgatcacccc
      481 ctcctccggg gggatgccgc gcaccagggc gaggtagcgc ttctccaccc gcccggcctc
      541 gaactgctcc tggaggcgcc gggtgatctc gggatcgaag gccaggagca ccacgccgct
      601 cgtcccccgg tcgagacggt ggacggggtg gacctggcgg ccgaggaggt cccgcaccag
      661 ggtcatgagc acgacccggt cctccgccca gccgcggtgg accgccaggc ccgacggctt
      721 ggcgaccgcg atcagggcgt catcctgata gaggaccggg aggggcggcg cggcgctcgc
      781 ggtgctcatc gcggcccccc tctcgatggc tccccgatca gccgacgcgg cccgcctgca
      841 ggaaggcgag gaccgagtag agcgtgccgg tggcgcccag caccaccatg atggtgaaaa
      901 agatcgtccg catcctgtcg tgctggagga tcaggctgat ggcgccgatc accagcgaga
      961 gctggaggaa gaaggtggag tagtcgaagg tgtcgccggc ggcgccgagc ccctctccca
     1021 ggctctccca ctcctgggcg cccgtcacct tccccatctc gccgtcgacg tcctgcaccc
     1081 agttggcctg gcccacggtc ttggagccga ggaggatctc cttcttctcc ttgtcgtagc
     1141 gctcggcctc ctgctccaga tgggcgatct gggactggac gccggtgagc tgggccggct
     1201 ggatggagcc cgcctccacc agcgccttga gggtgtcccg ctgcccctcg accaggctct
     1261 ccttgatgcc tttcgactgg taccaaagga acgccccgtt cttctcgttg tgcgcgatca
     1321 gctcgtcgtc gccgtacttg ccggccccca gatcggtcac cgcgagcacc gccgcgatca
     1381 gcgcgatgac gacgccgcac aggatctcga accgcttgct cccatcctcc gcctccgcca
     1441 tggagtcctc tcccattgga tgcccggagg caggcacgga gggagcaggc atgcgccgcc
     1501 caccgcggcc cgtcggcctc tccctcgaac gctgtccggg actgagggcc ctattgtagc
     1561 ggaggacgcc tcagtccgtc ggcaggttgg cggcgtcgcg cgcggccgcg gcggcgtcgc
     1621 cccgccgctt ggcgccgcgg gcgcggagaa tctcgatgac cgccggcagg atcgacacga
     1681 agatgatgcc caggatcacc agcgagaagt tctcctggac gaaaggcagg ttgccgaaga
     1741 ggtagcccgc catcgtgcag agcaccaccc agagcgccgc gccggcgacg ttgtaggtca
     1801 tgaagcgccc gtaggtcatg ctgccgagcc cggcgacgaa gggggcgaag gtacggacga
     1861 tggggacgaa gcgggcgatg acgatggtct tgccgccgta gcgctcgtag aagtcgtgcg
     1921 tccgctgcag gtgctcgggc ttgatgatcc gccgcatctt gagcaggcgc gggccgaggg
     1981 agtggccgat ccagtagttg accgtgtccc ccaggaccgc ggcgatgaag agcagcagca
     2041 tcagccacca cagcgagagc gagccggtgg cggcgaaggt gccggcggcg aacagcagcg
     2101 agtcgcccgg caggaacggc gtcaccacca ggccggtctc gcagaagacg atggcgaaga
     2161 gcagcccgta cgtccaggcc ccgtaggtct ccaggatcgg cttcagatgc ttgtcgagat
     2221 gcagaaagat gtcgatcagg tccttcaacg cttccattgt gctctccggg agaccgccgg
     2281 cgtgttccag caggcgccgg aagagtaagg gctacccgcc cggatggcaa ggggtgtgca
     2341 gagcaggagg caccccctcc gccacggggc cgttgcccgc gcaggaggtt ggtgacagac
     2401 tctcggacct tgctccactc cacacggaga gagctcacat gacgctttcg atcttcatcg
     2461 tcctggccct gctggtggcc gtggtcgtcg tcttcgcctt cgaatgggtg tcggtcgacg
     2521 tggccacgct gctgctgctg tcggctctgg tgctcaccgg catcctgtcg gtggaggagg
     2581 ccttttccgg cttctccaac gacatcatca tcattctcgc ctccatcttc atcctgtcgg
     2641 gggcgctgat gaagggcggg gtcatggacc gcctggggga cgcgatccac cgggtggccg
     2701 gcggcagccg caccaaggtg atgctcggcg tgctgccggt caccaccatc gtctcgtcct
     2761 tcatgaacaa cacgaccacg acggcgatcc tgatgccggc gaccctgggg ctctgccgca
     2821 agagcaagct gagcgccagc aaggtgctga tccccctggc ctacgcttcg atgctcggcg
     2881 gcacctgcac gctgatcggc acctcgacca acgtggcggc cagcggctac gtggcaagct
     2941 cgggcctcga gccgttctcc atgttcgagt tcctgcccgc ggggctcgcc atctgcctgc
     3001 tcggcatcgg ctacctgatc ctggtcggcc accggctgct gccggacgtg cgcgagaccg
     3061 gttacctgga ggagtacgcc atccgcgagt tcctctcgga ggtggtgatc accccgggct
     3121 cgccgctgtc gggccaggcg atccgcgaca gccgcctggc gcagatggac cttcaggtgc
     3181 gcgccgtgca ccgcggcgag cggcggctcg accccgagcc cgggctgcgc ctggaggagg
     3241 gggacctgct gatcgtccag accagccgcg aggggctgct gcagatcaag gagacggccg
     3301 gcatcgagat caagcccgac atcaccctcg ccgccgagga cctgaccagc gagacgatga
     3361 cgatcggcga ggcgatcctg atgcccggct cgtcgctcgc cggccgcagc gtcaaggagc
     3421 tcgatttccg gcgccgcttc ggcgtgatcg tgatcgcgat ctatcgccgt ggccacaccc
     3481 tggccacccg cctcggcacg ctgccgctgc gggtgggcga cgtgctgctc ctccaggggc
     3541 ggccggaccg cttccgcgcc ctcgccgaca actcggacct gtggatcctg caggaggtgg
     3601 agcaccggcc ggcccggcgg cgcaaggggt tctacgcgat ggggatcttc ctgctggcgg
     3661 tggcggcctc ggcgttcgag ctggtgccgc tgcccatcgc cttcctgctc gccgccctgg
     3721 gggtgatcgg cacgcggctg atcaccatgg aagaggccta cgacctcatc gactggcggc
     3781 tgctgatcct gatcgcaggc atgacctcct tcggcatcgc catgcgcaag accggcgccg
     3841 ccgagttcat cgccggcctg gtggtgcagt gggtctcgcc tctgggggtg acggcggtga
     3901 tgctcgcctt cgtgatcctc accatgctgc tgacgcagcc gatgtccaac gcggcggcgg
     3961 cgctggtggt gctgccggtg gcgatgcaca cggcgacgag cctcggcctc gatccccgca
     4021 ccttcgcggt gctggtgacg ctcgcggcct cgctctcctt catcaccccg ttcgagccgt
     4081 cgtgcctgat cgtctacggt cccggcaagt accggttccg cgacttcgtc acctgcggcc
     4141 tgccgctgac cgcgctggtg gtcgtctcgt tgatcctgct ggtgccgatg atctggccgc
     4201 tgcggcagta ggcggggtct acagccgccc gcggatccag cagccgccga ggacgcgggt
     4261 gccctgatag aagacggcgg cttggccggg ggtgacgccg cgctggggct cggcgaagcg
     4321 gacctcgacc tcccccttat ccaaaggacg gatgcgggcg gccacgccgg ggtggcggga
     4381 gcggatcttg accgtcgcct ccacctcgcc ggcctctccc agggacggat cggctccgat
     4441 ccagtggagg cgctccccct ggaggcctgg agcgagcagc gcctcctcct cccccaccac
     4501 cacccggttc tcctccggct cgatcgcgag cacatagaga gggctccggg cggccaggcc
     4561 gaggccgcgc cgctgtccca ccgtgtagcg atagtacggt tgcccggtcc ccagctcgcg
     4621 gcccgcggtg tccaccaggg tggacggcgc cggctcggct accgttccga agcgctccgg
     4681 gtgctcggcg acctgggcct cgacgaactc gcggacgccg ccggagacga agcagacctc
     4741 catgctctcc cccttcccgg ccacggcgag ccccgcctcc cgggcgagcg cccgcacctc
     4801 ccccttggtc atggcgccga gggggaacag ggaggcggcg agctgctcct gggtcagctc
     4861 gaagaggtag tagctctggt ccttgccggc gtcgacggcg gtgtgcagct cggggccgcc
     4921 ggggccgtcg aggatgcggg cgtagtggcc cgtcgccacc ctctccgccc ccagccggcg
     4981 cgcccgctcc agcagcaggt cgaacttgat ccaggtgttg cagcgcacgc aagggctggg
     5041 ggtgcgaccc tcgaggtagt cctggacgaa cgggtccacc acctggcgcc ggaagtcctc
     5101 gtccatgcgc agggtgtagt gggggatgcc ggcctgctgg gcgacgcgct tggcgtcccc
     5161 caggtcgagg gcgccgcagc agcggccgtg cgcctccacc ccggagtggt cccagaggag
     5221 catcgacaaa ccgatcaccg gccggtcccc cttcgacagc agccaggccg tcaccgacga
     5281 gtcgaggccg ccgctcatgg cgaccgcgag gagccccttc tgcggcccgc tcaccgtgcc
     5341 acctccagcg gcgccacccg gcgcagcgcc gccacctctc gggcgagggc ggcgaggaag
     5401 gcgtcgatct cctcggggcg gttggtcatg ccgacgctca cccgcaggga ggagagcgcc
     5461 tcctcggggg agaggccgat ggcgaggagc gtcttgctgg gctcgaccgc ccccgaggag
     5521 caggccgagc cggtcgacac cgcgaagccg gccaggtcga gccggatcag cagcgactcc
     5581 ccctcgacgc cgtgaaaggc gacgtgcgag gtgttgggca gccgcggcga gccctggcag
     5641 tggacggcgg cgtccgggat cgcgcgcagc cccgcctcga agcggtcgcg cagcgccgcc
     5701 aggcgcgccg cgcgctccgg cagctcggcg gcggtccgcg ccgcggcctc gcccagcccg
     5761 acgatccccg gcacgttctc ggtgctcgcc cggcgccggc gctcctggct gccccccacc
     5821 aggtagcccg gcagctccac ccccttgcgc acccacagcg ccgccacgcc cggcgggccg
     5881 tggaacttgt gggcgcccag ggtgaggtag tcggcgccca gcgcttcggc gctcaccggg
     5941 atcttgccca ccgcctgcac ggcgtcgcag agcaccggga tgccgcgcgc gcggcaggcg
     6001 gcggccacct cggccaccgg ctggaccgtc cccagctcgt tgttggcgag catcaggcag
     6061 accaggcgcg tgtccggccg gagcgccgac acgaccgcgg cggcggggac gacgccgtcc
     6121 gggccgggcc cgacccgcgt gaccgaccag ccgctctttt ccagccgcgc cgccgcctcc
     6181 cgcaccgagg ggtgctcgaa ggcggagatg accaggtgcc ccggaccatc ggcgcgggat
     6241 gaaatcccga agaggacggc gttgttggct tcagtccccg aggagg
//
LOCUS       DASPJI010000384         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_319247, whole genome shotgun sequence.
ACCESSION   DASPJI010000384 DASPJI010000000
VERSION     DASPJI010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319247"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VEL74_10190"
     CDS             <1..919
                     /locus_tag="VEL74_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HYG62940.1"
                     /translation="FSVGIAAAAFIAIFFFEVPFPAIVVGAGILGWLGRRFLPVPSPP
                     GTPASSSEAEPVVDVLATQIRPSLGRSLRVLAVCLTLWFVPLLAVRAALGPDHVLAVQ
                     GEFFSKMAVVTFGGAYAVLAYVAQQAVETYRWLGPGEMLDGLGMAETTPGPLIMVLQF
                     VGFMGAWRHPGGLDPLMAGILGSVLTTWVTFVPCFLWIFLGAPYVETLRNRKALSAAL
                     AGITAAVVGVILNLAVWFSLHTLFGEVAEVHRFGVRLLVPAWETLDPAALVLAAGAFL
                     ALFKFKRGMLETLALSAAAGLVYYLLSGR"
     gene            923..2008
                     /locus_tag="VEL74_10195"
     CDS             923..2008
                     /locus_tag="VEL74_10195"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYG62941.1"
                     /translation="MKRMLLLLMIGCLAGCASQPARPGTDPSIEGFAARLDALRQEAK
                     IPGLAVAVVRDGEVVLARGFGYADLEARVPVTEDTPFNIASVTKPLAGVVALRLVELG
                     RLDLDRPLTAYEEFAEWCADIQGAESIFFRDFNCATEALTERHLLTMTSNGKPGQRFF
                     YNPPAFSWASRPMMQVTGQAFSDLVAEHVFRPAGMTRSARQHRRLPLPPDTAAALAKP
                     YRLDESGNLVQADAPPPQGDGAAGGVISTARDLARFDLALDRDVLLKPESKAAMWRPT
                     VNSAGETLPYGIGWYVEQHEGRRLLWHSGWWEKAYSALYLKIPDERLALILLANSEGL
                     WWGKPLDKAEVQKSRFAAAFLEHFGRR"
     gene            complement(2009..>2269)
                     /locus_tag="VEL74_10200"
     CDS             complement(2009..>2269)
                     /locus_tag="VEL74_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020467190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG62942.1"
                     /translation="TVASALEAAESETFDAVVSDIGLPDGNGHDLMRRLVARFGLRGI
                     AVSGYGMEEDIQRSREVGFAAHLTKPVDLRRLRAELRELVGG"
BASE COUNT          282 a          846 c          771 g          370 t
ORIGIN      
        1 gttctcggtg gggatcgccg cggcggcgtt catcgccatc ttcttcttcg aggtgccgtt
       61 tccggcgatc gtcgtcggcg cggggatcct gggatggctc gggcggcggt tcctgccggt
      121 gccctccccg cccgggaccc cggcatcctc ttcagaagcc gagccggtgg tggacgtcct
      181 cgcgacgcag atccgcccat ccctcggccg gtcgctccgg gtgctcgcgg tctgcctgac
      241 gctctggttc gtccccctgc tcgcggtgcg ggcggccctc gggccggacc acgtgctcgc
      301 cgtccagggg gagttcttca gcaagatggc ggtggtgacc ttcggcggcg cgtacgcggt
      361 gctggcgtac gtcgcccagc aggcggtcga gacctaccgg tggctcgggc cgggcgagat
      421 gctcgacggc ctcggcatgg ccgagaccac cccggggccg ctcatcatgg tcctccagtt
      481 cgtcgggttc atgggcgcct ggcgccaccc cggcgggctc gatccgctga tggccggcat
      541 cctcggatcg gtcctcacca cctgggtgac gttcgtcccc tgcttcctct ggatcttcct
      601 cggcgcgccg tacgtcgaga cgctccgcaa ccgcaaagcg ctctccgccg ccctggccgg
      661 catcaccgcc gccgtggtgg gggtgatcct caacctggcg gtctggttct ctctccacac
      721 cctgttcgga gaggtcgccg aggtccaccg cttcggcgtc cgcctcctcg tccccgcctg
      781 ggagaccctg gaccccgccg ccctcgtcct cgccgccggg gccttcctcg ccctgttcaa
      841 attcaagcgc gggatgctcg agaccctggc cctcagcgcc gcggccgggc tcgtctatta
      901 cctgctctcc gggagataga tcatgaagcg gatgctcctc ctcctgatga tcggctgcct
      961 cgccggctgc gcctcccaac cggcccggcc cgggacggac ccctcgatcg agggcttcgc
     1021 cgcccgcctc gacgctctcc ggcaggaggc gaagatcccc ggcctcgccg tcgccgtggt
     1081 gcgggacggc gaggtggtcc tcgcccgcgg attcggctac gcggacctgg aggcccgggt
     1141 gccggtgacg gaggacacgc cgttcaacat cgcgtcggtc accaagcccc tcgccggcgt
     1201 cgtcgccctg cggctcgtcg agctgggccg gctcgacctc gaccgcccgc tcacggccta
     1261 cgaggagttc gcggagtggt gcgccgacat ccagggcgcc gagtccatct tcttcaggga
     1321 cttcaactgc gccaccgagg ccctgaccga gcgccacctg ctgaccatga cctccaacgg
     1381 caagccgggc caacggttct tctataaccc gccggccttc tcctgggcgt cgcgccccat
     1441 gatgcaggta accgggcagg cgttctcgga cttggtggcc gaacacgtgt tccggcccgc
     1501 cggcatgacc cgctcggcgc gccagcaccg ccggctgccg ctgcctcccg acaccgccgc
     1561 cgccctggcg aagccgtacc ggctggacga gagcggcaac ctggtgcagg cggacgcccc
     1621 gccgccgcag ggggacggcg cggccggcgg cgtcatctcg acggcgcggg acctggcacg
     1681 cttcgacctc gcgctggacc gcgacgtcct cctgaagccg gaatcgaagg cggcgatgtg
     1741 gcgaccgacg gtcaatagcg cgggagagac tctcccttac ggcatcggct ggtacgtgga
     1801 gcagcacgag gggcgccggc tgctctggca ctccggctgg tgggagaagg cctactcggc
     1861 gctctatctg aagattccgg acgagcggct cgcgctcatc ctgctggcca acagcgaagg
     1921 cctctggtgg ggaaaacccc tggacaaggc cgaggtccag aagtcccgct tcgcggcggc
     1981 gttcctggag cacttcggcc gccgctgatc agcccccgac gagctcccgc agctcggcgc
     2041 gcaggcgccg gaggtccacc ggcttggtga ggtgggcggc gaagccgacc tcgcggctgc
     2101 gctggatgtc ctcctccatg ccgtagccgc tgacggcgat ccccctgagg ccgaagcggg
     2161 cgacgagccg gcgcatcagg tcgtggccgt tgccgtcggg caggccgatg tcgctgacca
     2221 cggcgtcgaa cgtctcggac tccgccgcct ccaatgccga ggcgacggt
//
LOCUS       DASPJI010000385         4772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_196139, whole genome shotgun sequence.
ACCESSION   DASPJI010000385 DASPJI010000000
VERSION     DASPJI010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196139"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1715)
                     /locus_tag="VEL74_10205"
     CDS             complement(<1..1715)
                     /locus_tag="VEL74_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HYG62943.1"
                     /translation="MFEKYNEKARRALFFARYEASKLGSRVIESEHILLGILREGEET
                     VTEIFRRFQAKPEDIRREIEGERVFVERISSTAELPLSEESKKILAYASHEAESMLHA
                     TVGSEHLLIGILRVEGCTAMRILSQHGFDVYTVREEVLAIAKEREASQQKKELPFLSE
                     YGRDLTALAAEGSFDPLIGRDDEVDRIVQILSRRTKNNPILLGEPGVGKTAIVEGLAD
                     RIVEGKVPIFLANKRIVALDLSLIVAGTKYRGQFEERLKGILKELKESRELIVFIDEI
                     HSLIGAGSAEGSLDAANILKPALSRGEISCIGATTLKEYRKYIEKDRSLLRRFQAIQV
                     NPPTPEQTLEILDGVKDRYESFHKVRYRPEALRTAIYQSSRYIPDRHLPDKAIDVIDE
                     AGARVKLRRVRDTQNLRRLEQDIRQVVKEMKVAISDKDFERAVYLREREIELREDLER
                     MSVTIDETGTLEVTRHDVEDVISSWTGIPVAALQSEEAERLLRMEDTLKQRVIGQDRA
                     ISAISRAIRRSRLGVSNPQRPVGSFIFLGPSGVGKTEVARQLSEFLFGSQRALVRFDM
                     SEYME"
     gene            complement(2044..2721)
                     /locus_tag="VEL74_10210"
     CDS             complement(2044..2721)
                     /locus_tag="VEL74_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG62944.1"
                     /translation="MSVTVRSIGLTKTYQDGVRRVEVLKGIDMTIEPGEMVAVVGPSG
                     SGKSTLLHLLGGLDRADAGSVEIGGQTLSGLSSSRLAAFRNRTIGFVFQFHQLLPDFT
                     ALENVMLPGRIAGLEPKGVLDNARRLLLEVGLGERLEHFPNQLSGGERQRVALCRALA
                     LEPPLLLADEPTGNLDPASGEQVFQLLLDLQARHGTTGILVTHNPEIAGRCTRTLRLE
                     SGLLLPY"
     gene            complement(2718..3920)
                     /locus_tag="VEL74_10215"
     CDS             complement(2718..3920)
                     /locus_tag="VEL74_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYG62945.1"
                     /translation="MARSSSLLLRKLPFPVVLAMRYLRSTRRDAFASFLSAVAAGGIA
                     LGVAALILSLAVISGFQNALRGELLSRTPQIEIELPPGLGDEAAALETVRRVPGVASA
                     DLQVQGGGWLVAGDNVDAVLLVGYDGPVPSTFPGAQGLPPGLYVPTALAARWGLAPGK
                     TVNIVSPRPTLTPFGPQPRIRSLTLAGTYDSGRTQEDRERVALPRAVAETLLGTSGRQ
                     ITVTAADLDAALEVAESLGPVLPEGSVVRTWKDLNRPLLFALMLEKALMFVAVSLIVL
                     VAALALVADLALIISNKRPEIGILGTMGATPAALRNAFLILGGMVSGSGALLGTILGA
                     GGAWVLDRYRLLPVPGRVYFLDYVPFLVQGRDLALVLLLTVTLAFASAFYAAQRAASL
                     DPVEALRR"
     gene            complement(3933..>4772)
                     /locus_tag="VEL74_10220"
     CDS             complement(3933..>4772)
                     /locus_tag="VEL74_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HYG62946.1"
                     /translation="MRIAPELYLKRLLVGGIPRVYEINRNFRNEGISTRHNPEFTMLE
                     FYWAYADYRDMMDFTEEMVVELAEKVLPADVAGQGPALTWKGERIELSRPFARFTMRD
                     AVAHFAGIAPERLDSADTLREELAARRLPVPPGGTYGHLLMALFEETVEEKLTAPTFI
                     TDHPVEVSPLAKQRPDDPRFTERFELYIGGMELANAFSELNDPDVQAERFQQQIAARE
                     RGDQEAHLFDADYVRALEHAMPPAAGNGTGIDRLTMLFTDRASIRDVILFPLMRPEQD
                     AAG"
BASE COUNT          720 a         1606 c         1714 g          732 t
ORIGIN      
        1 ttctccatgt attcgctcat gtcgaagcgg accagggccc gctggctgcc gaacaggaac
       61 tcggagagct ggcgcgccac ctcggtcttg ccgacgcccg aggggccgag gaagatgaac
      121 gagcccaccg gccgctgcgg gttgctcacc cccaggcggg agcggcggat cgcccggctg
      181 atggcgctga tcgcccggtc ctggccgatg acccgctgct tgagggtgtc ctccatgcgc
      241 agcagccgct cggcctcctc gctctgcagc gccgccaccg ggatgccggt ccacgacgag
      301 atgacgtcct cgacgtcgtg ccgcgtcacc tccagggtgc cggtctcgtc gatcgtcacg
      361 ctcatccgct cgaggtcctc gcgtagctcg atctcccgct cgcgcaggta gacggcgcgc
      421 tcgaagtcct tgtcggagat cgcgaccttc atctccttga ccacttggcg gatgtcctgc
      481 tccaggcggc gcaggttctg ggtgtcgcgc acccggcgca gcttgacgcg ggcgccggcc
      541 tcgtcgatga cgtcgatcgc cttgtccggc aggtggcggt ccgggatgta gcggctcgac
      601 tggtagatcg ccgtgcgcaa cgcctccggg cggtagcgca ccttgtggaa ggactcgtag
      661 cggtccttga ccccgtcgag gatctccagc gtctgctccg gcgtcggcgg gttgacctgg
      721 atcgcctgga agcggcgcag cagcgaccgg tccttctcga tgtacttgcg gtactccttg
      781 agcgtcgtcg cgccgatgca ggagatctcg ccgcgcgaga gggccggctt caggatgttg
      841 gccgcgtcga gcgacccctc ggccgagccg gcgccgatca gcgagtggat ctcgtcgatg
      901 aagacgatca gctcgcggct ctccttgagc tccttcagga tccccttgag ccgctcctcg
      961 aactggccgc ggtacttggt gccggcgacg atcagcgaca ggtcgagcgc cacgatccgc
     1021 ttgttggcca ggaagatcgg gaccttgccc tcgacgatgc ggtcggccag accctcgacg
     1081 atggcggtct tgccgacgcc cggctcgccc agcaggatcg ggttgttctt ggtgcggcgc
     1141 gacaggatct ggacgatgcg gtcgacctcg tcgtcgcggc cgatcagggg gtcgaacgac
     1201 ccttcggcgg cgagagcggt caggtcgcgg ccgtactcgg agagaaacgg cagctccttc
     1261 ttctgctgcg aggcctcgcg ctccttggcg atcgccagca cctcctcgcg gacggtgtac
     1321 acgtcgaagc cgtgctggga gaggatgcgc atcgcggtgc acccctcgac gcgcaggatg
     1381 ccgatcagga ggtgctccga gccgacggtg gcgtggagca tgctctccgc ctcgtgggag
     1441 gcataggcca ggatcttctt ggactcttcg gaaagcggca gctccgcggt ggaggagatg
     1501 cgctcgacga agacccgctc cccttcgatc tcgcggcgga tgtcctcggg cttggcctgg
     1561 aaccgccgga agatctcggt caccgtctcc tcgccctcgc ggaggatgcc cagcaggatg
     1621 tgctcggatt cgatgacgcg gctgcccagc ttgctggctt cgtagcgggc gaagaagagg
     1681 gctctgcggg ccttctcgtt gtacttctca aacatgctgt tcgacccctc ggtgatacgc
     1741 gacggccctg caccaaacct ccaaaactcg gtgcggacga ggtcttctgc gcgccgtctt
     1801 caagttcccc gattctaaca cacgcctctt ccccggaacg gaagggcctg aaaggaggcc
     1861 cctccgcccc atccaccgcg ttggacgcca ctgacgcgga ccgcacggac ggggcatctc
     1921 acctcttgga agccggggag gagcggccaa aattcccggc gggacgaacc acgtcgaaga
     1981 gaccccgccc cgcccgctgc tcacagtttt tgccccgatg cactggtgtc aatgtttctt
     2041 ttgtcaatag ggcaggagca ggccgctctc cagacgcagg gtgcgggtac agcggcccgc
     2101 gatttcgggg ttgtgggtga ccaggatgcc ggtggtgccg tgccgtgcct ggaggtcgag
     2161 gaggagctgg aagacctgct cgccgctcgc cgggtcgagg ttgccggtag gctcgtcggc
     2221 gaggagcagc ggcggctcga gcgccagggc gcggcagagg gcgacacgct gccgctcgcc
     2281 gccggagagc tggttcggga agtgctccag gcgttcgccg aggccgacct ccagcaggag
     2341 gcggcgggcg ttgtccagca cccccttggg ctccaggccg gcgatccgcc cggggagcat
     2401 gacgttctcc agggcggtga agtcgggcag gagctggtgg aactggaaga cgaaaccgat
     2461 ggtgcggttg cggaaggccg cgaggcgcga gctcgacagg ccggacaggg tctggccgcc
     2521 gatctcgacg ctgccggcgt cggcccggtc gagcccgccc agcaggtgga gcagcgtcga
     2581 cttgccggag cccgaggggc cgaccacggc caccatctcg ccgggctcga tcgtcatgtc
     2641 gatccccttg agcacctcca cccgccggac gccgtcctgg taggtcttgg tgaggccgat
     2701 ggagcggacg gtcacgctca tcggcggagc gcctccacgg gatcgagcga cgcggcgcgc
     2761 tgggcagcgt agaaggcgga ggcgaaggcc agcgtcacgg tgagcagcag gaccagcgcc
     2821 aggtccctcc cctgcaccag gaagggcacg tagtccagga aatagacccg gccgggcacc
     2881 ggcagcagcc ggtagcggtc gagcacccag gcgccgccgg cgccgaggat cgtgccgagc
     2941 agggcacccg agccggacac catgccgccg aggatcagga aagcgttgcg cagcgccgcc
     3001 ggggtcgccc ccatggtccc gagtatgccg atctccggcc gcttgttgga gatgatgagc
     3061 gccaggtccg ccaccagcgc cagggcggct accagcacga tcagcgacac ggcgacgaac
     3121 atcagcgcct tttcgagcat cagagcgaag aggagcggcc ggttgaggtc cttccaggtg
     3181 cggaccacgc tcccctccgg caggaccggc cccaggctct cggccacctc gagggcggcg
     3241 tcgaggtccg cggccgtgac cgtgatctgg cgcccggagg tgccgagcag ggtctcggcc
     3301 acggcgcgcg gcagggcgac ccgctcgcgg tcctcctgcg tccggccgct gtcgtaggtg
     3361 ccggcgaggg tcaggctgcg gatgcgcggc tgcggcccga acggcgtcag ggtggggcgc
     3421 ggcgacacga tgttcaccgt cttgccgggg gcgagccccc agcgggcggc cagggcggta
     3481 gggacgtaca gccccggcgg cagcccctgg gcgccgggga aggtggaagg caccggaccg
     3541 tcgtagccga cgagcagcac cgcgtcgacg ttgtccccgg ccaccagcca gccgcccccc
     3601 tgcacctgga ggtcggccga agccacaccg ggcacccggc ggaccgtctc gagggcggcg
     3661 gcctcatccc cgagccctgg cggcagctcg atctcgatct gcggcgtccg ggagagcagc
     3721 tccccacgca gggcgttctg gaagccggag atcaccgcca gggagaggat cagcgccgcc
     3781 acgccgaggg cgatcccccc ggcggccacc gccgacagga agctggcgaa ggcatcccgc
     3841 cgcgtgctcc gcaggtagcg cattgcgagg accaccggga agggcagctt ccgcagcagc
     3901 agtgacgagg atctggccat gaggggctgg cgtcagccgg cggcgtcctg ctcgggccgc
     3961 atgaggggga agaggatgac gtcgcggatc gacgcccggt cggtgaacag catggtgagc
     4021 cggtcgatgc ccgtgccgtt gccggcggcc ggcggcatgg cgtgctccag cgcccgcacg
     4081 tagtcggcgt cgaacaggtg ggcctcctgg tcgccgcgct cgcgggcggc gatctgctgc
     4141 tggaagcgct cggcctggac atccgggtcg ttcagctcgg agaaggcgtt ggccagctcc
     4201 atgccgccga tgtagagctc gaaccgctcg gtgaaccgcg gatcgtccgg ccgctgcttg
     4261 gcgagggggg atacctccac cgggtggtcg gtgatgaagg tgggggcggt gagcttctcc
     4321 tccaccgtct cctcgaacag cgccatgagg aggtggccgt aggtgccccc gggcggcacc
     4381 ggcagcctgc gggccgccag ctcctcgcgc agggtatctg ccgagtccag ccgctcgggg
     4441 gcgatgccgg cgaagtgggc cacggcgtcg cgcatggtga agcgggcgaa gggacggctg
     4501 agctcgatcc gctccccctt ccaggtgagc gccggcccct ggcccgcaac atccgccggg
     4561 agcaccttct ccgccagctc gacgaccatc tcctcggtga agtccatcat gtcccggtag
     4621 tcggcgtagg cccagtagaa ctcgagcatg gtgaactcgg ggttgtgccg ggtggagatc
     4681 ccctcgttgc ggaaattgcg gttgatctcg tagacccggg ggatgccgcc gaccagcagc
     4741 cgcttcaggt agagctccgg cgcgatccgc at
//
LOCUS       DASPJI010000386         2237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_768029, whole genome shotgun sequence.
ACCESSION   DASPJI010000386 DASPJI010000000
VERSION     DASPJI010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768029"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2237
                     /locus_tag="VEL74_10225"
     CDS             <1..>2237
                     /locus_tag="VEL74_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG62947.1"
                     /translation="EAVDATLDLAVLSVGADVWPVQDMERAVRRVAPHTRLVNSYGVT
                     EASVDSSLCDCPLVRPFAGPVVPIGRPFPGTRLLVLDTALRPVPAGVSGELFLGGPVL
                     ARGYFGRPDLTAGRFVPDPFPGLDGQPGARLYRTGDRARLLPDGVVELLGRVDHQIKV
                     RGVRIEPGEIEAALAGQTGVAQAVVVAAGPAADRRLVAFVVPVDPDSPPSVSSLRIGL
                     RQRLPEALVPAAFEVLDSLPLTSNGKVDRRALGLLAAESGTPESSPAKPRTPVEEIVA
                     GIFAEVLGEAAVVGIQDDFFELGGHSLLATQVVARLRAVFGVDLPLRRLFDVPTVAGL
                     AAEIAALLLESPSEVPPLVPVPRSGGEALPLSFAQERLWFLEQLFPGAGLYHMPLVAR
                     LAGPLDAAALAWSLAAVVERHEILRIVCPAVEGRPVQRVVPARGIALPRIDLAALPGP
                     QREAAAREWVEEASVRPFELGEAPPWRALLLRLGPEDHVLALVLHHIAADGWSLEVLI
                     RDLSALYAGTPLQELAVQYTDFAVWQREWLQGEVLEGQLHYWRERLAGLPPALDLPFD
                     RPRPAIPSGRGAVRGFTLPRLAVPLRGLGRQSGATLFMALLAGVEALLARLSGQTDIA
                     VGTAVAGRERVETEGLIGLFINTLVLRCDLADDPAFLPVLLARAREAALGAYAHQTLP
                     FERLVEELQPERAFSYTPLFQVMLALQNTPAAGLSLPGLRAEPAQAERRAAAFELGLQ
                     LFELG"
BASE COUNT          223 a          851 c          811 g          352 t
ORIGIN      
        1 ggaggcggtg gacgccaccc tcgacttggc ggtgctctcg gtcggggccg acgtctggcc
       61 ggttcaggac atggagcggg cggtccgccg cgtcgcccca cacacccggc tggtcaactc
      121 ctatggcgtg accgaggcga gcgtcgactc gagcctctgc gactgtccgc tcgtccgccc
      181 gttcgccggg ccggtggtcc ccatcggccg tcccttcccg gggacgcgcc tcctggtcct
      241 cgacaccgcc ctgcggccgg tgccggccgg cgtgtccggc gagctcttcc tcggcggccc
      301 ggtcctggcc cgcggctact tcggacggcc ggacctcacg gccgggcgct tcgtgcccga
      361 tccgtttccg ggactggacg gtcagcccgg tgcccggctc tatcggacgg gcgaccgggc
      421 gcggctcctg ccggacgggg tggtcgagct gctcggccgc gtcgaccacc agatcaaggt
      481 gcgcggcgtg cgcatcgagc cgggggagat cgaagcggcg ctggccggcc agaccggggt
      541 ggcccaggcg gtggtggtcg ccgccggacc ggcggccgac cgccggctgg tcgcgttcgt
      601 cgtccctgtg gacccggact cgccgccctc cgtctccagc ttgcggatcg gtctgcggca
      661 gcggctgccc gaggcgctgg tcccggcggc gttcgaggtt ctcgacagcc tccccctgac
      721 gtccaacggc aaggtggacc gccgcgctct cggcctgctc gcggccgaga gcggcactcc
      781 ggagagctcg ccggccaaac cgcgcactcc cgtagaggag atcgtggcgg ggatcttcgc
      841 cgaggttctc ggcgaggccg cggtcgtggg gatccaggac gatttcttcg agctcggcgg
      901 ccactctctc ctcgccaccc aggtggtggc gcggctgcgg gccgtcttcg gggtggacct
      961 gccgttgcgc cgcctgttcg acgtcccgac cgtggcgggc ctggcggcgg agatcgcggc
     1021 cctgctcctg gagagcccct ccgaggtccc gccgctcgtg cccgtgccgc ggagcggcgg
     1081 cgaggccctg cctctctcgt ttgcccagga gcgcctctgg ttcctggaac agctcttccc
     1141 gggcgccggc ctctaccaca tgcccctggt ggcgcggctc gccggtccgc tcgacgccgc
     1201 cgccctggcc tggagcctgg ccgcggtggt ggagcgccac gagatcctgc gcatcgtctg
     1261 cccggcggtg gagggccggc cggtgcaacg cgtcgtaccc gcgcgaggga tcgccctccc
     1321 ccgcatcgac ctcgccgccc ttcccggccc acaacgcgag gccgccgccc gcgagtgggt
     1381 ggaggaggcg tcggtccgtc ccttcgagct gggagaggcg ccgccctggc gggcgctgct
     1441 gctccgcctc ggccccgagg accatgtcct ggcgctggtg ctccaccaca tcgcggcgga
     1501 cggctggtcg ctggaggtcc tgatccgcga cctgtccgcc ctttatgccg gaacccccct
     1561 gcaggagctg gccgtgcagt acaccgactt cgcggtctgg cagcgcgaat ggctgcaagg
     1621 ggaggtcctc gaggggcagc tccactactg gcgtgagcgt ctggccggcc ttccaccggc
     1681 cctcgacctg ccgttcgatc gtccccgccc ggcgatcccg agcggccggg gcgcggtgcg
     1741 cggctttacc ctgccgcgcc tcgccgtacc gctgcgcggt ctcggccggc agtcgggcgc
     1801 gaccctgttc atggccctgc tcgccggtgt cgaagccctg ctggcccgcc tctcgggcca
     1861 gacggacatc gcggtcggca ccgccgtcgc cggccgtgag cgggtggaga ccgaggggct
     1921 gatcggcctc ttcatcaata ccctggtgct gcgctgtgac ctggcggacg accccgcctt
     1981 cctgcccgtc ctgctggccc gggcccgcga ggcggcgctc ggcgcctacg cccatcagac
     2041 cctgcccttc gagcgtctgg tcgaggagct gcagccggag cgggcgttct cctacacacc
     2101 gctcttccag gtgatgctcg cgctccagaa cacccctgcg gccgggctgt cgctgccggg
     2161 gctcagggcc gagccggcgc aggccgagcg ccgggcggcc gcgttcgagc tgggcctcca
     2221 gctcttcgag ttggggg
//
LOCUS       DASPJI010000387         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_319437, whole genome shotgun sequence.
ACCESSION   DASPJI010000387 DASPJI010000000
VERSION     DASPJI010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319437"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VEL74_10230"
     CDS             <1..213
                     /locus_tag="VEL74_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62948.1"
                     /translation="YMVGSMFLNTAYSELIYHLIALSVCLEIVAHRETAEAAEGAAAE
                     RPDAASDAGQPWWKRPLPQPSLSGRG"
     gene            220..2178
                     /gene="asnB"
                     /locus_tag="VEL74_10235"
     CDS             220..2178
                     /gene="asnB"
                     /locus_tag="VEL74_10235"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HYG62949.1"
                     /translation="MCGIAGLFHYRGESSGAGFARRETLASPLTAMVGCIRHRGPDDQ
                     GFHVEGPFGLGHARLSIVDLAGGHQPIFNEDRSIAVVLNGEIYNHRALRQELEGRGHR
                     FATRSDTEVIVHLYEELGEGCVARLAGMFTIAVADLRHRRLLLARDRAGKKPLYLADD
                     GRRLGFASELKSLLAAGLAAPEIDPEALDLYLTLGYVPSPWSIVRGARKLEAGHLAVC
                     DARGVRIERYWDVEARPQESLTLAQWTEELEFLLREAVRDRLESEVPLGAFLSGGIDS
                     GTVVALMSEALGEPVRTHTVGFHEPGFDEREDAAAVARALGTDHMEFEVRPDLVDVLP
                     RIAWHLDEPFADPSAVPTWYVSRETRRRVTVALSGDGGDELFAGYPGRYGQPALEARA
                     RRLVPGPVRQHLFPWLSRRWPRSPRLPRALRLGGFLGNVAVDADRAWFRDRHHVPPHL
                     LERLFGHRLAERRGRFDPWAAVEPHVSRAPQGDPLSRLLYLDFKTWLADDGLVKVDRM
                     SMAHGLEVRCPLLDHRVVELAARVPSRFKMADGRTKILLREVAARHLPAEILSRPKRG
                     FAPPVDRWLRHELRDLSRDLLLGSDAMARGLFERRELERLLADHQARRLEAGWAIWTL
                     LMLEVWGRAVLRSEQPSLPQEVSHAAGA"
     gene            2162..>2486
                     /locus_tag="VEL74_10240"
     CDS             2162..>2486
                     /locus_tag="VEL74_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYG62950.1"
                     /translation="MPPAPEPSRKTRVLLLSSVFPNAAQPVHGVFVRERMRGLPEELE
                     VRVVAPTPWFPLVSGLRPGFRPRVPPQEEQGGVEVLHPRFLSVPAVAKCLDGVLMFLS
                     LLPVLL"
BASE COUNT          287 a          899 c          916 g          384 t
ORIGIN      
        1 tacatggtgg gcagcatgtt cctgaacacc gcttactccg agctgatcta ccacctgatc
       61 gccctgtccg tctgcctcga gatcgtggcc catcgcgaga ccgccgaggc cgccgagggg
      121 gcggcggcgg agaggcccga cgctgcgtcc gacgcggggc agccctggtg gaagcgtccg
      181 ctgcctcagc cgagtctttc cgggagggga tagccaatca tgtgcggtat cgccggtctg
      241 ttccactatc ggggggagag ctccggcgcc ggcttcgccc ggcgcgagac gctggcttcg
      301 cccctcaccg cgatggtggg gtgcatccgc caccgcggcc ccgatgacca ggggttccat
      361 gtcgagggtc cctttggcct gggccacgcc cgcctgagca tcgtcgactt ggcgggcggc
      421 caccagccga tcttcaacga agaccgctcc atcgccgtgg tgctgaacgg cgagatctac
      481 aaccaccgcg ccctgcgcca ggagctggag ggccggggcc accgcttcgc cacccgctcg
      541 gacaccgagg tgatcgtcca cctctacgag gagctggggg aagggtgcgt cgcgcggttg
      601 gccggtatgt tcaccatcgc cgtggccgac ctccggcatc gccggctgct gctggcgcgc
      661 gaccgggcgg gcaagaagcc gctctatctc gccgacgacg gccggcggct cggtttcgcc
      721 tccgagctca agagcctgct ggccgcgggc ctcgccgcgc ccgagatcga tcccgaggcc
      781 ctggacctct atctcacgct cggctacgtg ccgtccccct ggtcgatcgt ccgcggtgcg
      841 cgcaagctgg aggccggcca tctggcggtc tgcgacgcgc gcggcgtgcg catcgagcgc
      901 tactgggacg tggaggcccg gccgcaggaa tcgctgacgc tggcgcagtg gaccgaggag
      961 ctcgaattcc tgctgcggga ggcggtgcgc gaccggctgg agagcgaggt gccgctgggg
     1021 gcgttcctct ccggcggtat cgactcgggc acggtggtgg cgctgatgag cgaggccctg
     1081 ggcgagccgg tgcgtactca taccgtcggc ttccacgagc cgggcttcga cgagcgcgag
     1141 gacgccgccg cggtggcccg cgccctgggc accgaccata tggagttcga ggtgcgcccg
     1201 gacctggtgg acgtcctgcc gcgcatcgcc tggcacctgg acgagccctt cgccgacccg
     1261 tcggcggtgc ccacctggta cgtgtcgcgc gagacccgcc ggcgggtgac cgtggccctg
     1321 tcaggggacg gcggcgacga gctgttcgcc ggctatcccg gccgctacgg ccagccggcg
     1381 ctggaggcgc gggcgcgccg cctggtgccg ggcccggtgc gccagcacct gttcccctgg
     1441 ctgtcgcgcc gctggccccg ctcgccccgg ctcccgcggg cgttgcgcct cggcggcttc
     1501 ctgggcaacg tggcggtgga cgccgaccgc gcctggttcc gcgaccgcca ccacgtgccg
     1561 ccccacctgc tggagcgcct cttcggccac cgtctcgccg agcggcgcgg gcgcttcgac
     1621 ccctgggccg ccgtcgagcc gcacgtctcc cgggcgcccc agggcgatcc gctgtcccgc
     1681 ctgctctacc tggatttcaa gacctggctc gccgacgacg gtctggtcaa ggtggaccgg
     1741 atgagcatgg cgcacggcct ggaggtgcgc tgcccgctgc tcgaccaccg ggtggtggag
     1801 ctcgccgcgc gggtgccgtc gcgcttcaag atggcggacg ggcgtaccaa aatcctgctg
     1861 cgcgaggtgg cggcgcgcca tctgccggcg gagatcctgt cccggccgaa gcgcggcttc
     1921 gcgccgccgg tggaccgctg gctgcggcac gagctgcgcg acctgtcgcg cgacctgctg
     1981 ctggggtccg atgccatggc ccgcggcctc ttcgagcgcc gcgagctgga gcggctcctg
     2041 gccgaccacc aggcgcggcg gctggaggcg ggctgggcga tctggacgct actgatgctg
     2101 gaggtatggg ggcgcgcggt gctccgctcc gagcaaccgt cgctacccca ggaggtgagc
     2161 catgccgccg gcgcctgagc cgtccaggaa gacccgggtg ctgctgctga gcagcgtctt
     2221 ccccaacgcg gcgcagccgg tgcacggcgt tttcgtgcgc gagcggatgc gcggcctgcc
     2281 ggaggagctg gaggtgaggg tggtggcgcc caccccctgg ttccccctcg tgtcgggcct
     2341 ccgcccgggc ttccggccgc gggtgccccc gcaggaggag cagggcggcg tggaggtcct
     2401 ccacccccgc ttcctctcgg tgcccgcggt ggccaagtgc ctcgacggcg tgctgatgtt
     2461 cctctctctg ctgccggtgc tgctgc
//
LOCUS       DASPJI010000388         8398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_426386, whole genome shotgun sequence.
ACCESSION   DASPJI010000388 DASPJI010000000
VERSION     DASPJI010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8398)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426386"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2284
                     /locus_tag="VEL74_10245"
     CDS             <1..2284
                     /locus_tag="VEL74_10245"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYG62951.1"
                     /translation="IELYDGALVSRDGRSTAVLVGAPDGVDRTPFHGAVRETAQSHQG
                     GGDTLEVVGAPVAEALLGSHILADLGVPPALLGQAAGDYESNRPGLMPLVLAIMALVF
                     LAVFRHPLAALSPVVMIGACLAFTFGVMGWLGIPVYLTTVVLPVILSAVGVADQIHIF
                     LRYRELRRERPDLTPAALAGATMDEMASPVVQISVTTIAGFLSFAFSPLAPVRAFGVF
                     AAVGSLASLTWSLMVIPALLTLAHPSFGKRGQDQRPAGATSRRGFAGLARVATRRRAA
                     VLAGALLSALVAGDGVRRLRVQDSWVDGFAPESRFAQAMRRFDHDFLGTHLLQITVEA
                     DPLTFQGEIAGSAVDHKRLVLPARQMPAGADPVRLAGSRIEVRQLPAPDRPAPLDWSG
                     SVESARRQGDRLVLDLPLRGGSPRFWLEPRPDERLRYAVHLEPLLAPAVLRRVGDLEA
                     FLAGRPGVGGVRGPAGYLEATGFMKAPHRPDSRALPDRTETARALWSSYGFVRGPERL
                     RQLVDPEYTRALVTVFLKAPDYAGTRRLMAEVRDYERRHLAPHGLRLGFAGDVAVSQA
                     LIGAVVTTQVRSLALSLVGIFAVTALLGRSLRRGLYCVVPPTFAILLSFAAMGWLGIP
                     LGVATSMFAAMTLGIGVDYSIHLLERHRRAREEGLAGEAALTEALAATGPAIAVDTLS
                     VGLGFAVLLLSQVPANARLGGIVALSLVICLAATLVVVPALVGRRGDLRARSEPLEPR
                     RLDQPRVTGDEEDREAEPVA"
     gene            complement(2189..2581)
                     /locus_tag="VEL74_10250"
     CDS             complement(2189..2581)
                     /locus_tag="VEL74_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG62952.1"
                     /translation="MKRYVAEPESGAIRNFFEDGMCAALRLSEAEVASALARRCREGA
                     FPESERDRALTALRQDFGSLLIVEVTGEVVARSVTLLVRHRLRAADALQLSSCLELRD
                     RLRLPVLFIACDARLVEAARFEGLGTGP"
     gene            complement(2608..2904)
                     /locus_tag="VEL74_10255"
     CDS             complement(2608..2904)
                     /locus_tag="VEL74_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYG62953.1"
                     /translation="MEKRSIGSRELKTRLGTYLRQVQEGMTLIVTDRGRPVAELRPYE
                     PHDGDLEDRLYELDALGVVSWEVRENEPLPAFRPIISRGKSASEAVLEDREDRF"
     gene            2990..4723
                     /locus_tag="VEL74_10260"
     CDS             2990..4723
                     /locus_tag="VEL74_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HYG62954.1"
                     /translation="MLRRILNKKQDELLAENRHLVAELQKALARFDAAEEDQATLERS
                     ARQLDELFLLVVAGEFNAGKSAFINALLGDRILEEGVTPTTSRVHLIKHGPQVGSTVV
                     ESALDVVTAPVDLLQDINIVDTPGTNAIHREHEAITREFVPRSDMVLFVTSADRPFTE
                     SERAFLQGIREWGKKVVVVVNKIDILETPEDWERIRVFIADNAKALLGFTPDIFPVAA
                     KPALRGKQSGDKELLASSRFEALERYIVETLDEKERVRLKLMNPLGVAEALSRKYLQI
                     TDRRLELLKEDFSAIEDIERQLTVYREDMGREFRFRLSDVDKVLHEFENRGVEFFDET
                     MRITRVFDLVNKSKMKADFESKVVGDAPQVIERRVSEVIDWIVASDLRQWQAVMEHLD
                     KRRHAHADRIVGKVGGSFDFDRSRLLDSVGRAAQRSVETYDREAEANRMADSVQVAVA
                     GTALVEVGAIGLGAIITHVAVTAAADFTGILAASTIAVLGLFIIPNRRHEAKKDLREK
                     IEAMRKDLMGALTGTFDREIEQSLRRIDEAIGPYTRFVRAERQRLDELRGELRGVDEG
                     IARLRARVESL"
     gene            4770..6380
                     /locus_tag="VEL74_10265"
     CDS             4770..6380
                     /locus_tag="VEL74_10265"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYG62955.1"
                     /translation="MSEESAPTPIPQKRPGTFQSIARAFTSWRTASVTLMSFASGMPL
                     GLVWLAIPDWMRDIGVDIRVVGLFTLAQVPWTFKFLWSPLMDRFVPPLGRRRGWAIIA
                     QIGLFVSTLALAGVGSHPEAPWVVGAIALAIAFAGATQDIAIDAYAVDVLRPEEQSVA
                     VGARNALWRLGYYIAGSISITLAGTWGWPAVNVFLACLYLPMILVLRKSPDPGSVGAP
                     PRTLKEAVWFPFLGFLARHRALEILAFVLLYKLADNLVQSLQRPFLIDMGYNEFDRGV
                     ALTIIGLFANILGTLIGGLLTAPLGLGRALWIFGFFQIFSNIGYAILAGEPGVNRPMM
                     WGAFGFEALCSGLGSGAFSVMLLRMTQKRFSATQYALFSSLFGLPRIVSGPIAGFTAY
                     AIGWEDFFWSTLALGIPGLIMLARFVPWSAREVTFAVEPPKIRQPLTGAQLTLRGLAG
                     AAVGFASGALLLSFLEALRVMKDSPGTAFDMWTPLIAMGRPQDQGAWIGLVGLLVFGA
                     CCGLFTAAVVAARHGAGQDLAMDSSGDV"
     gene            6466..6678
                     /locus_tag="VEL74_10270"
     CDS             6466..6678
                     /locus_tag="VEL74_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HYG62956.1"
                     /translation="MSLQRSRQNRMIAGVCGGLADWLGWSPTVVRIAYVLVSILSAGF
                     PGILVYLILWLAMPKAPRGGWATGQV"
     gene            6807..>8398
                     /gene="ade"
                     /locus_tag="VEL74_10275"
     CDS             6807..>8398
                     /gene="ade"
                     /locus_tag="VEL74_10275"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HYG62957.1"
                     /translation="MDLPRLLAVARGDQPADLLLRNARLVNVFSGEVYLTEIAIAGPL
                     ISGIGEGLAARRELDLDGAFVAPGLIDAHVHIESSLVPPAELSRAVVPRGVTTVITDP
                     HEIANVMGLDGIRFMLRDSEGIALDVRVNAPSCVPATHLETSGAHLEADDLARLLGEP
                     RLLGLAEVMNFPGVIGGDPGILAKLRAFQGRPVDGHCPGVTGKGLDAYAAAGIRNDHE
                     SVTAEEALEKLRRGLAVFIREASNARNLRALLPLVTPAAERRICLCTDDRQPGDLLGE
                     GSIDHMVREAIAGGVDPVTAIRLATLNTAEHYGLTDRGAVAPGRLADLIVFPDLRAPR
                     AERVFRAGALVAKEGRLLVEPPRRSYDDLPRTVHVDLGRLDFSIPAQPGPARVIGVVP
                     DQLITEDLRLEPAIRDGLADADPERDLLKMAVVERHRGTGATGLGFVKGVGLRRGAIA
                     GTVAHDHHNLVVIGADDVSMCTAARALAEAGGGFAVAEGEEVRALVPLPIAGLMSDRP
                     IEEVRDRMRELLAAARDLGASLR"
BASE COUNT         1148 a         3064 c         2854 g         1332 t
ORIGIN      
        1 catcgagctc tacgacggtg ccctcgtctc ccgggacggg aggagcacgg cggtcctcgt
       61 gggcgccccc gacggcgtgg accggacccc cttccatggc gccgtccggg agaccgcgca
      121 gagccaccag ggaggcggag acaccctcga ggtggtcggt gcgcccgtgg ccgaggctct
      181 gctgggaagc cacatcctgg ccgatctggg ggtgccgccg gcgctgctcg ggcaggccgc
      241 cggagactac gagagcaatc gcccgggttt gatgcccctc gtgctggcga tcatggccct
      301 cgtcttcctg gcggtcttcc ggcaccctct ggccgcgctc tccccggtag tgatgatcgg
      361 agcctgcctg gcgttcacct tcggcgtgat gggctggctc gggatcccgg tgtatctcac
      421 cacggtggtg ctgccagtga tcctatcggc cgtgggggtg gccgatcaga tccacatctt
      481 tctccgctac cgcgaattgc ggcgggagag acccgatctc acccccgccg ccctggcggg
      541 cgccaccatg gacgagatgg cctcgccggt ggtccagatc tccgtcacca ccatcgccgg
      601 cttcctctcg ttcgcgttct cgccgctggc gccggtgcgg gccttcggtg tgttcgcggc
      661 ggtgggcagc ctggcgagcc tgacgtggtc tctgatggtg atccccgcgc tgctgacgtt
      721 ggcgcacccg tctttcggga aacgggggca ggatcagcgg ccggccggcg ccacgtcgcg
      781 gcgaggcttc gccggtctcg cccgggtcgc cacccgccgg cgagctgccg tgctggccgg
      841 agccctcctg agcgccctgg tggccggcga cggtgtacgc cgtctccggg tgcaggacag
      901 ttgggtcgac ggcttcgctc cggagagccg ctttgcccag gccatgcggc ggttcgacca
      961 cgacttcctg ggcacccacc tcctccagat caccgtcgag gccgatccac tcaccttcca
     1021 gggagagatc gccgggagcg ccgtggacca caagaggctg gtcctacccg cccggcagat
     1081 gccggcgggc gccgacccgg tccggctggc cgggagccgg atcgaggtgc ggcagctccc
     1141 ggctccagac cgcccggccc ctctcgactg gtcgggctcc gtcgaaagcg cccgccggca
     1201 gggggaccgg ctggtcctcg acctcccgct gcggggcggc tcaccgcgct tctggctgga
     1261 gccccggccg gacgagcggc tgcgctacgc ggtgcatctg gagccgctcc tggcacccgc
     1321 cgtcctgcgc cgggtcggcg acctcgaggc gttcctcgcc ggcaggcccg gcgtgggtgg
     1381 cgtccgcggg cctgccggct atctggaggc gacgggcttc atgaaggcgc cccaccgtcc
     1441 cgattcacgc gctctgcccg accggaccga gacggcgcgc gccctctggt cgagctacgg
     1501 cttcgtgcgt ggtcccgagc gcctgcgcca gctcgtcgat cccgagtaca cccgggccct
     1561 ggtcaccgtc ttcctcaaag ctcccgacta cgccggcacc cggcggctca tggcggaggt
     1621 gcgggactac gagcgccggc acctcgcgcc gcacggcctg cgcctgggct tcgccggcga
     1681 tgtcgccgtg agccaggccc tgatcggcgc ggtggtgacg acgcaggtcc gctcgctggc
     1741 gctctccctg gtggggatct tcgcggtgac ggccctcctc ggacggtcgc tccgccgcgg
     1801 cctctattgc gtggtgccgc ccaccttcgc gatcctcctg agcttcgccg ccatgggttg
     1861 gctgggaatc cccctgggag tcgccacctc catgttcgcc gccatgacgc tggggatcgg
     1921 agtggattac tccatccacc tcctggagcg ccaccgccgc gcccgggagg agggcctcgc
     1981 cggggaggcg gcgctgaccg aggccctggc cgccaccggg ccggccatag cggtcgacac
     2041 cctgagcgtc ggcctgggct tcgcagtgct gctcctctcc caggtgccgg ccaacgcccg
     2101 gctcggaggc atcgtggccc tgagcctggt catctgcctg gccgcgacgc tggtggtggt
     2161 gccggcgctg gtggggcggc ggggcgatct acgggcccgt tccgagcccc tcgaaccgcg
     2221 ccgcctcgac cagccgcgcg tcacaggcga tgaagaggac cgggaggcgg agccggtcgc
     2281 gtagctcgag gcaggaggag agttggagag cgtccgcggc gcggagccgg tgacggacga
     2341 gcagggtgac gctccgcgcc accacttctc ccgtcacctc cacgatcaga agggacccga
     2401 aatcctgccg aagagcggtc agggcccgat ccctctcgga ctccgggaac gcgccttcac
     2461 gacagcgccg ggcgagggcg gacgcgacct cggcctcgga gaggcgtagc gcggcacaca
     2521 tcccgtcttc aaagaagttg cggatggctc cgctctccgg ctccgccacg tagcgcttca
     2581 ccagcgcgct ggcatcgaag tacaggatca gaagcgatcc tcgcggtctt ccagcacggc
     2641 ctcgctggcg ctcttgcccc gactgatgat cggcctgaaa gcaggcagcg gctcgttctc
     2701 ccggacctcc cagctcacca cgcccagagc atccagctcg tacagccggt cctccagatc
     2761 cccatcatgc ggctcatagg gccgcaactc ggcgacgggc ctgccgcggt cggtcacgat
     2821 caaggtcata ccctcctgga cttggcggag ataggtgccg agtcgcgtct tgagctcgcg
     2881 agaaccgatg ctccgtttct ccatgtggtc attgtaacca ccagccttga gcgaagcaag
     2941 agcaccctgg cacaccccat gctatcgtct ccacgggagg cggggggtca tgctacggcg
     3001 cattctcaac aaaaaacagg acgagctgct ggcggagaac cgccatctgg tagccgagct
     3061 ccagaaggcg ctcgcccggt tcgacgccgc cgaggaggac caggcgaccc tcgagcggtc
     3121 ggcgcggcag ctcgacgagc tgttccttct cgtcgtggcc ggcgagttca acgccggcaa
     3181 gagcgccttc atcaacgccc tgctcggcga ccggatcctc gaggaggggg tcacccccac
     3241 caccagccgt gtccatctca tcaagcacgg gccccaggtc ggcagcaccg tggtcgagtc
     3301 cgccctggac gtcgtgaccg cgccggtcga cctgctccag gacatcaaca tcgtcgacac
     3361 cccggggacc aacgccatcc atcgcgagca cgaggcgatc acccgcgagt tcgtgccgcg
     3421 ctcggacatg gtcctcttcg tcacctccgc cgaccgcccg ttcaccgaga gcgagcgcgc
     3481 cttcctccag ggcatccgcg agtggggcaa gaaggtcgtg gtggtggtca acaagatcga
     3541 catcctggag acgccggagg actgggagcg catccgcgtc ttcatcgccg acaacgccaa
     3601 ggccctcctc ggcttcaccc ccgacatctt cccggtcgcc gccaagcccg ccctgcgcgg
     3661 caagcagagc ggcgacaagg aactgctcgc cagcagccgc ttcgaggccc tcgagcgcta
     3721 catcgtcgag accctggacg agaaggagcg cgtccgcctc aagctgatga acccgctcgg
     3781 cgtcgccgag gcgctgtcgc gcaagtacct ccagatcacc gaccggcgcc tcgagctgct
     3841 caaggaggac ttctcggcca tcgaggacat cgagcgccag ctcaccgtct accgggagga
     3901 catgggccgg gagttccgtt tccgcctgtc ggacgtggac aaggtcctcc acgagttcga
     3961 gaaccgcggc gtcgagttct tcgacgagac catgcgcatc acccgcgtct tcgacctggt
     4021 caacaagtcg aagatgaaag cggacttcga gagcaaggtg gtgggcgacg cgccgcaggt
     4081 catcgagcgg cgggtgtccg aggtcatcga ctggatcgtc gcctcggacc tgcgccagtg
     4141 gcaggcagtc atggagcacc tggacaagcg ccgccacgcg cacgccgacc gcatcgtcgg
     4201 caaggtcggc ggcagcttcg acttcgaccg cagccgcctg ctcgacagcg tcggccgcgc
     4261 tgcccagcgc tcggtcgaga cctatgaccg ggaggccgag gccaaccgca tggccgactc
     4321 ggtgcaggtg gcggtggccg gcaccgccct ggtcgaggtg ggggccatcg gcctcggcgc
     4381 catcatcacc catgtcgcgg tcaccgccgc ggccgacttc accggcatcc tcgccgccag
     4441 caccatcgcg gtcctcggcc tgttcatcat ccccaaccgc cggcacgagg ccaagaagga
     4501 cctgcgcgag aagatcgaag ccatgcgcaa ggacctgatg ggggcgctga ccggcacgtt
     4561 cgaccgcgag atcgagcaga gcctgcggcg catcgacgag gcgatcggcc cctacacccg
     4621 cttcgtgcgc gccgagcgcc agcgcctgga cgagttgcgc ggcgagctcc ggggggtgga
     4681 cgaggggatc gcccgcctgc gcgcccgggt cgaatcgctg taagctcctc cggttgcggc
     4741 ccggcgtgcc gcggaggagc gtcgagacct tgagcgaaga atccgccccg actccgatcc
     4801 ctcagaagcg gcccggcacc ttccagagca tcgccagagc cttcacctcc tggcgcaccg
     4861 cctcggtgac cctcatgtcc ttcgcctcgg gcatgcccct ggggctggtc tggctcgcca
     4921 tccccgactg gatgcgcgac attggggtcg atatccgggt ggtcggcctg ttcaccctgg
     4981 cgcaggtgcc gtggaccttc aagttcctct ggtcgccgct gatggaccgc ttcgtgcccc
     5041 ccctcggccg gcggcggggg tgggcgatca tcgcgcagat cgggctcttc gtctcgaccc
     5101 tcgccctggc cggcgtcggc agccacccgg aggccccctg ggtggtgggg gccatcgccc
     5161 tcgccatcgc cttcgccggg gcgacgcagg acatcgccat cgacgcctac gcggtggacg
     5221 tgctgcgtcc cgaggagcag tcggtggcgg tgggcgccag gaacgccctc tggcggctcg
     5281 gctactacat cgcgggcagc atctcgatca ccctcgcggg gacctggggc tggccggcgg
     5341 tcaacgtatt cctcgcctgt ctctatctgc cgatgatcct ggtgctccgg aagtcgcccg
     5401 acccgggctc ggtgggcgcg ccgccgcgga cgctcaagga ggcggtctgg ttcccgttcc
     5461 tcggcttcct cgcccgccac cgcgcgctcg agatcctcgc cttcgtcctc ctctacaagc
     5521 tggcggacaa cctcgtccag tcgctgcagc ggccgttcct catcgacatg ggctacaacg
     5581 agttcgaccg cggcgtcgcc ttgaccatca tcgggctctt cgccaacatc ctcggcaccc
     5641 tgatcggcgg gctgctgacg gcgccgctcg ggcttggccg ggcgctctgg atcttcggct
     5701 tcttccagat cttctcgaac atcggctacg ccatcctcgc cggcgagccg ggggtcaacc
     5761 ggccgatgat gtggggcgcc ttcggcttcg aagcgctgtg cagcggcctc ggcagcggcg
     5821 ccttctcggt gatgctcctg cgcatgactc agaagcggtt ctcggccacc cagtacgccc
     5881 tcttctccag cctcttcggc ctgccgcgca tcgtctcggg tcccatcgcc ggcttcaccg
     5941 cttatgccat cggctgggag gacttcttct ggtcgacgct ggcgttgggc atccccggcc
     6001 tgatcatgct ggcccggttc gtcccctgga gcgcgcgcga ggtgaccttc gcggtggagc
     6061 cgccgaagat ccggcagccc ctgaccggcg cgcagctcac gctgcgcggg ctcgccggcg
     6121 ccgccgtggg gttcgcctcc ggcgccctgc tcctctcctt cctcgaggcg ttgcgggtga
     6181 tgaaagacag cccggggacg gcattcgaca tgtggacgcc gctcatcgcg atgggccggc
     6241 cgcaggatca gggagcctgg atcggcttgg tggggctcct ggtcttcggc gcctgctgcg
     6301 gcctgttcac cgccgcggtg gtggcggcgc ggcatggcgc gggccaggac cttgcaatgg
     6361 actcttccgg cgacgtctga ccgtcgcccg gcgaaaccgt ggcggccctc tcctcgtaat
     6421 agaggtgggg gccggcggcg ccggcccgaa aggaggaaga agccgatgtc actccaacgt
     6481 tcccgccaaa accggatgat cgccggcgtc tgtggcggcc tggccgactg gctgggctgg
     6541 agcccgacgg tggtccgtat cgcctacgtc ctggtctcga tcctctccgc gggcttcccg
     6601 gggattctcg tctacctgat cctctggctg gcgatgccca aggcgccccg cggcggctgg
     6661 gccaccgggc aggtctagga tctcacgagc cgggggaggc acgcgcctcc cccatgcctc
     6721 ttcattccgc atacccattg ccggagtagg accgggacgg tgactggcga tatgctgggc
     6781 cctccttccg ccgggaggac ctctccatgg atctgccacg cctcctcgcc gtcgcccggg
     6841 gtgaccagcc cgcggacctg ctgctgcgca acgcgcggct ggtgaacgtc ttctcgggcg
     6901 aggtctacct cacggagatc gccatcgccg gcccgctgat ctccgggatc ggtgagggct
     6961 tggcggcgag acgggagctg gacctggacg gcgccttcgt cgctccaggg ctcatcgacg
     7021 cacacgtcca catcgaaagc tctctcgttc cccccgccga gctctcccgg gcggtggtcc
     7081 cgcgcggagt caccaccgtc atcaccgacc cgcacgagat cgccaacgtc atggggctcg
     7141 acggcatccg cttcatgctg cgggactcgg agggaatcgc gctggatgtg cgggtcaacg
     7201 ccccgtcctg cgtgccggcc acccatctgg agacctcggg agcgcatctg gaggctgacg
     7261 atctggcgcg cctcctcggc gagccccggc tcctgggtct cgccgaggtc atgaacttcc
     7321 ctggagtgat cggcggcgat cccggcatcc tcgccaagct ccgcgccttc caggggcggc
     7381 cggtcgacgg ccactgcccc ggcgtcaccg gcaagggcct cgacgcctat gccgccgccg
     7441 gcatccgcaa cgaccacgag tcggtgacgg cggaggaggc gctggagaag ctgcggcggg
     7501 ggcttgccgt gttcatccgc gaggcgagca acgcgcggaa cctgcgggct ctcctccccc
     7561 tggtcactcc ggcagccgag cgccggatct gtctctgcac cgacgaccgc cagccaggcg
     7621 acctcctggg agagggttcc atcgatcaca tggtgcggga ggcgatcgcc ggcggcgtcg
     7681 atccggtgac cgccatccgc ctcgcgaccc tcaacacggc cgagcactac ggcctcaccg
     7741 accgcggcgc cgtggcgccg ggccgccttg ccgacctcat cgtcttcccg gacctccgcg
     7801 ccccccgggc cgagcgggtg ttccgggcgg gcgcgctggt ggccaaggag ggccgcctcc
     7861 tcgtggagcc cccgcgccgt tcctatgacg acctgccgcg cacggtgcac gtcgatttgg
     7921 gccggctgga cttctcgatc ccggcccagc cgggaccggc gcgggtcatc ggcgtggtgc
     7981 cggaccagct catcaccgag gatctgcggc tggagcccgc catccgcgat ggcctggccg
     8041 atgcggaccc ggagcgcgac ctcctgaaga tggcggtggt ggaacgccac cgcggcaccg
     8101 gtgccacggg cctcggcttc gtcaagggtg tgggcctgcg gcggggcgcc atcgccggca
     8161 ccgtggccca cgaccaccac aacctggtgg tgatcggcgc cgacgacgtc tccatgtgca
     8221 ccgccgcccg cgccctggcc gaggccgggg gcggcttcgc ggtcgccgag ggcgaggagg
     8281 tgcgagcgct ggtgccgctg cccatcgccg ggctgatgtc cgaccggccg atcgaggagg
     8341 tgcgcgaccg tatgcgcgag ctgctggccg ccgcccgcga cctgggcgcc agcctgcg
//
LOCUS       DASPJI010000389        18097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_832896, whole genome shotgun sequence.
ACCESSION   DASPJI010000389 DASPJI010000000
VERSION     DASPJI010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18097)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18097
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832896"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VEL74_10280"
     CDS             <1..493
                     /locus_tag="VEL74_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62958.1"
                     /translation="PETRKAYDRGLDPKARATLVAAIAPTQDASRDLARGYYQRASSL
                     VDAEDYHFAVELLQQAVNIDPRGEYFALLGRIQARNPQWLEDAAASLQRAIDLGTPDR
                     SLPDALAEVRRLIATGEASEEEMEISVVEVEEEDEAPRKPAPKPRTGSKRGRGKAAGK
                     PRG"
     gene            519..931
                     /gene="rnpB"
                     /locus_tag="VEL74_10285"
     ncRNA           519..931
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VEL74_10285"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            934..1899
                     /locus_tag="VEL74_10290"
     CDS             934..1899
                     /locus_tag="VEL74_10290"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HYG62959.1"
                     /translation="MIRSARLPPPVGPGDRVGVAALSGPVDPGRLAAGMDALRRLGFE
                     PVPADNLASRHGFFAGTDAERLAAFHRLAADPDLPAILFARGGHGLLRLLPAIDWDLL
                     RRRPRAYVGYSDLTPLLLGIVERLGWVTFHGPLVAGDLARGLSADEEASFLGVLAGRY
                     PVVEPLGRVLRGTASGRGAAAAGPFVGGCLSLLAAVQGTPYFPDLEGALLFWEDINEP
                     PYKIDRMLTQLGLSGRLRAIEGMIVGHLGWTEAPVTGGGAEGGAVGTTDWPAVVAESV
                     AGFDWPLVWGLESGHLRPNHTLPLGLGARLEARPDAAAGEIVFEG"
     gene            1903..2712
                     /locus_tag="VEL74_10295"
     CDS             1903..2712
                     /locus_tag="VEL74_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYG62960.1"
                     /translation="MANPVFKTTGSGAAKKSSGANIIDLKAGEYIFQEGDLGTEMYII
                     HEGKVEILSQAAGEEQLLAVLEKGDFFGEMSVLEDMPRAASARALTHSVLLQINGSTF
                     DQMLQANPEIAVRMMRKLSRRLRETDELLRGLQQLQGTAIRSAAGGARELPKPMVQVP
                     AEGPERLVHAPSGMEFPLSTSSETTIGRKDPVTGIYPDVDLTPIDGQRSVSRRHSKIY
                     RRGTKFFLAEEIGTMNSTYLNGARLDTGVPAELKPGDEVRCGVVILRFEVP"
     gene            2736..6011
                     /gene="recG"
                     /locus_tag="VEL74_10300"
     CDS             2736..6011
                     /gene="recG"
                     /locus_tag="VEL74_10300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HYG62961.1"
                     /translation="MDLGAPLRRVPGVGPARASALSNAGLVTVRDLLFHLPVRYEDRR
                     QARRVAELVPDEATTVRGWLRDVRRLRTRRRGFSIVRSVLDDGTGQIPVVWFNRPYLA
                     GQAAGEGEGEWILHGKVREGKGGPELLNPSCEPARGPEALHAGQIVPVYPSAGSVGPA
                     ALRRILDAVLGEVDLPAQVQETLPAELLERYHLPSLGEALEALHRPGPDADAGLLERR
                     RSPAHLRLIFGELLELQLALALLRRRELAEPRRLRYRLGRPGARLEKRLRELPPFPLT
                     AAQERAVREILADLRAPHPMLRLLQGDVGSGKTVVAALALAAALENGYQGAFMAPTEM
                     LAEQHFASLERLLGGRYRLGLFTGSRRDPALLAALAAGEVQLAVGTHALIQDGVAFQR
                     LALAVVDEQHRFGVIQRGLLRRKGERPDLLVITATPIPRSLALAAYGDLPVSVLDEMP
                     PGRPPVETRVVPAARRRQVYKVLREDLMEGGRAYVVFPLIEESGQVAAASLTEMGERV
                     RRLLSGIPSAVVHGRMPAAERERAMRAFATGEARVLIATTVVEVGVDVPEATWMVIES
                     AERFGLAQLHQLRGRVGRGTQPSRCLALYGPLSDAARRRLEVFQETTDGFVIAQADLE
                     MRGPGDILGTRQAGLPALRVADPVRDFEWLERARDVVGELLVRLGEPGWRISSTAPAP
                     CCRRAEEPGEENGTMTINHPPAEPLRVLLVANTLPPRDVSGVGEQVLQLAAGLRDAGC
                     EVEILGRGPGGVAGPKVLFPLFVVPALRRALARFRPHVVQVHESDGGLAALAASVVAP
                     LLEPRPLVAALLQVSYVEELRAVRPLVSSGGRALGRPGGVERRFRLGKAPLQILLGWL
                     TAWVADVVLAPSAATAGELRRDYGVAEVEVVPNVTGGLEIEPPRDEPGEAPSGYLLFV
                     GRLRIRKGVEVLLEALRDFPGARLLIAGDGEHRGALERKAAELDLGPAAVFLGRCDAS
                     RVRGLLRHAAALVVPSIYEGMPLVVLEAMEAGVPVVASRVSGIPEVVEDGRTGWLVEP
                     EDPAALAAALAAVLNEDGEARRRGEAGRLRVEERFRPAKAAARWLELMRPKIRESGRG
                     TLP"
     gene            6008..6544
                     /locus_tag="VEL74_10305"
     CDS             6008..6544
                     /locus_tag="VEL74_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62962.1"
                     /translation="MKLGRALLAVGVIATLGLIATAVLGYMLPGPADPAVPRHVLAAL
                     VASLLLLFSHCWILLYLIATGRAIREAVREYGVEASLEAEAWRLRRGVLPWLLLAAAT
                     ALGTFLLGAAAMAGAASADAHHILFYVTLVVQALALWIESRGLAANERLLGDIDQRLG
                     MAPTPPAPPVSAGARGAA"
     gene            6541..7233
                     /locus_tag="VEL74_10310"
     CDS             6541..7233
                     /locus_tag="VEL74_10310"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HYG62963.1"
                     /translation="MSPERLFTGGVEILVLLLAISAHESAHAWTADRCGDSTARSLGR
                     VSLNPLRHLDIFGSLLLPLLQVSFQAPVFGWGRPAPVQPQSFRDPRRGELLVAAAGPF
                     ANVLLAAVAVFGLLIAVHVLGPDARRAAYYTLMQQTGSAADLPGFPVVFTLVRMATIN
                     AFLVVFNLIPLPPLDGGQIALYMLPPDWAAKLAGVRPYGFIIGIALAAFGVVTLLLIP
                     FYGVLSVVIHLL"
     gene            7252..8298
                     /gene="trpS"
                     /locus_tag="VEL74_10315"
     CDS             7252..8298
                     /gene="trpS"
                     /locus_tag="VEL74_10315"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HYG62964.1"
                     /translation="MATESKKIVLSGMRTTGKIHLGNYFGALKNWVGLQDQYRCYYMA
                     ADWHALTSDYADPSQVSSNTLAMVADWIAAGLDPEKSVIFVQSMVPDHAELHLLLSMM
                     APLGWLERVPTYKEQIQQLENKDLSTYGFLGYPLLQTVDIIIYRAHFVPVGEDQASHI
                     EISRELVRRFNHLYGGGIFPEPQALFTPTPKVPGIDGRKMSKSYGNAINLSDPPDVIR
                     DKCKQMFTDPQRLTRKDPGRPEVCNLYEFHKLMSPADVQERVYHQCRAAEIGCVDDKK
                     LLAQIIIDFLEPMQRRRHELERDPNMLMDILVEGSRRARERTAETLDMVRGALGLDYS
                     RLRGAVPGSAGSVG"
     gene            8295..9167
                     /locus_tag="VEL74_10320"
     CDS             8295..9167
                     /locus_tag="VEL74_10320"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HYG62965.1"
                     /translation="MSRPRRIVPKPAPGAEAGEGAPVAPGMETAASGSLLPEAWRVQL
                     PVFEGPLDLLLHLIRINKVEITDIPVATICDQFHAYLGLMEELNLDVAAEYIYEAALL
                     IHLKSKLLLPRPAKAAGEPEEDPRQELVERLLEYRRMKEVAQSFAEVDRLRMGIWTRK
                     PQPLPQVEEEEEQAIDMGEVSLFDLLSALRQAMVRYDKEHPPPLQLSGEAFSVRGQFD
                     RLLAHLDAGHPYDLINDLRGLSCRAEAIATFLAVLELARLNLIRVHQTEVGDVLLYRT
                     TRELSDRDWEEIGG"
     gene            9164..9874
                     /gene="scpB"
                     /locus_tag="VEL74_10325"
     CDS             9164..9874
                     /gene="scpB"
                     /locus_tag="VEL74_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HYG62966.1"
                     /translation="MTERHEMESAIEAILFVSSEPVPRAKLLEMFDEEEREQANEALN
                     AVLARFEAGDGRGVMAEDVAGGVRLVTRPEAVGWLRRFFDVAGGSKLSMAALETLAII
                     AYRQPITGPEIQELRGVSSAGVIKTLLERRMVRIAGRKEVVGKPFLYGTTREFLVHFG
                     LNSLKDLPPLEEFEETFGPGGEATMALSAAALEAVAPPGPVPLAGEDREERILQEVAA
                     IEEAASEAPDPDEGPGEE"
     gene            9911..11035
                     /locus_tag="VEL74_10330"
     CDS             9911..11035
                     /locus_tag="VEL74_10330"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HYG62967.1"
                     /translation="MSEERLQKILARAGVAARRKAEELIRDGLVTINGQVAELGSKAD
                     PERDAIKVDGKRIHPSAAHRYLLLNKPKGVMSTVSDPEGRPTVIDLVPPGLRRALVPV
                     GRLDFDTEGLLLLTDDGDFAQQVAHPRFGCTKTYEVKVKGRPEESQLERLRTGIVLDG
                     RRTAPCRITPHSRTITATRRRGRFDDGDDNTTWWMVELNEGRTRQIREMFHRIGHPVQ
                     KLRRVAIGPVRDPRLPVGAVRELSEQEVEKLTKASGGPKPAPGAKKAPAKKVAAKKTA
                     AKKGTAGTEAAKAEKRPRGERRTLAEVSRESSRAPKGPKVTAAGRKVMAGARKGAAPT
                     AKATPAAKAGPRKGSPRTGGGAKDPAGPRSSGPRSGHGRG"
     gene            11049..11729
                     /gene="cmk"
                     /locus_tag="VEL74_10335"
     CDS             11049..11729
                     /gene="cmk"
                     /locus_tag="VEL74_10335"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005390185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HYG62968.1"
                     /translation="MRPLIVAIDGPSGVGKSTTARRLARRLGVPFLDTGAMYRAIGVK
                     VLESGIDPADQKGVEAVAAAVDVQLGHLDDGSFEVLLDGQPVEHRIRTPEVSEATSAI
                     SVYPEVRRRLVALQQEAARQFGGVLEGRDIGTHVFPDTPYKFYLDADPEVRGRRRYDE
                     LVAAGRQVVYGDVVEDIRRRDHRDSTRADSPLTCLPSHIFIDASNLTVDEVVATMERA
                     IEERGGEG"
     gene            11748..11984
                     /locus_tag="VEL74_10340"
     CDS             11748..11984
                     /locus_tag="VEL74_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62969.1"
                     /translation="MSALRAPQSRPVVVLQRPPLGAEVAEYMDGRGPDITSVWWMPRA
                     WPSSWGAVVWMSRPLPVVCHFWAALKEMSDSTMT"
     gene            complement(12154..12402)
                     /locus_tag="VEL74_10345"
     CDS             complement(12154..12402)
                     /locus_tag="VEL74_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62970.1"
                     /translation="MTETLSRLALRVFVVGVLLVWAVPSQAYRMIQNRSVGRVSASAA
                     AACDVSNGFVHWENQQIEWFVNHRRRRIDRRPASRRRP"
     gene            complement(12511..13287)
                     /locus_tag="VEL74_10350"
     CDS             complement(12511..13287)
                     /locus_tag="VEL74_10350"
                     /inference="COORDINATES: protein motif:HMM:NF015709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HYG62971.1"
                     /translation="MTCRALILVVVLASGCATAPTPTAAPPAQPAPAATPSQTAPSQA
                     APAAPPAAIHWVRDSAEHHAALLQAYLLATERIEKLAKGVPAGTWAVALDGDETVIDN
                     SDYMKERAAQGAGFDSASWKQWTEKKAATPLPGAVAFLTRVHELGGKIAIVTNRSNAE
                     CPDTEADFRNDGIPYDLMLCMENDSEKEPRWQKVENGTAAPGVPPLRILLWVGDNIKD
                     FPDLDQSLLAKQDSAYSDFGRRFILIPNPMYGSWERNPAR"
     gene            13439..13984
                     /gene="lysM"
                     /locus_tag="VEL74_10355"
     CDS             13439..13984
                     /gene="lysM"
                     /locus_tag="VEL74_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HYG62972.1"
                     /translation="MGLIDFAKSVGRKLGLGDDDDKKPQGQKVPGPGAQAAPTAQQVE
                     ELKERRKAAALVKLVQEMGFKVQDLGIRVDNDRVTLTGTADTQENREKIVLLVGNVEG
                     IGSVDDQLKVSTPAPEATYYEVKSGDTLSKISKQFYGDANKYMVIFEANKPMLKDPDE
                     IYPGQRLRIPAAVESAAGRHS"
     gene            complement(14039..15181)
                     /locus_tag="VEL74_10360"
     CDS             complement(14039..15181)
                     /locus_tag="VEL74_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62973.1"
                     /translation="MPFMRPLLLAALALLVSLPTAAAPLAICDGSTAVTLLGMDTASG
                     RMLFSIPSTGEDGRGWLVELDPEGREARAWPNPPKGLFGGSVGPGPVLAVTSCGTSCL
                     QPMRWTGGASDSGWEPLGDPLTSPTAATVTPTYDNTGAPWVILHGASRQDGQIQAWAF
                     RMEGREWKNVGGQMVTAVGQPSALPAPQRKDGVLTGTALFSSSGRPEVWVTGVPSLPA
                     ERRGQIIALTGTSAAYVSADGVVYLSDDSGKGWRRSTWTPWGTQSTAGMWRQGTDYWV
                     DLPVGDHRGALRLVWFDRRRPTEETVVLTRLAPSGDWIRLSETRSEVRSKSGEQLPVT
                     QVLVPKGEIWLLLSGCAATAQGSGFVLRSFDGTALSASRFVPFRTR"
     gene            complement(15202..16305)
                     /locus_tag="VEL74_10365"
     CDS             complement(15202..16305)
                     /locus_tag="VEL74_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009373861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HYG62974.1"
                     /translation="MDLFQQMASMGHERVLVCSNPEVGLEAIIAVHSTALGPGLGGVR
                     MWPYKSMDDAVTDVLRLSRGMTYKAAAAGLNLGGGKAVIIGDPKKDKSEALFRSFGRY
                     VESLGGLYITAEDVGSDMEDMEVVLEETRWVTGVSPAHGGGGDPSPVTAYGVLQGIKA
                     ASHWQLGGNDGVLTGRSVAIQGLGSVGYHLAKYLKDEGAKVFGADIDTEATAKANEDL
                     GVEIVPTAEILSVDCDIVAPCALGAILNDQTIPALRCKVVAGAANNQLADEAQHGQAL
                     HDRGILYAPDFVINAGGLINVYNELTGYNRERAMRMTRGIYLNMMRVFQISRDQSVPT
                     YVAADRVAEERIAKVKSMRAQHWGRTIREARRG"
     gene            complement(16498..16968)
                     /gene="bcp"
                     /locus_tag="VEL74_10370"
     CDS             complement(16498..16968)
                     /gene="bcp"
                     /locus_tag="VEL74_10370"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HYG62975.1"
                     /translation="MSDWIEEGQTAPDFTLAADDGRQVKLSELRGKPVVLYFYPQDDT
                     PGCTKEACAFRDRRQDLADRGAVVLGVSPDDVASHGRFRDKYSLNFPLLADTGHQVAE
                     QYGAWREKNMYGKTSMGIQRSTFLIDADGKVRKVWKRVSVDGHDEEVLAALGKL"
     gene            complement(17032..17949)
                     /locus_tag="VEL74_10375"
     CDS             complement(17032..17949)
                     /locus_tag="VEL74_10375"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG62976.1"
                     /translation="MPKRDTPKIAAPSSASRHILNGCLTGDTHPALGLVRLLDELGSV
                     EAVADLIGEAVATGHPVAAELEGLLLDHRDACERITSMLRHHPAPAPPSPPGTAVDEI
                     RRFFDRAVRQDEVASVAAYSLGSPELLEQATEEILVLLDRWGLLDPDRSALEIGCGIG
                     RVLAALAPRLAEIHGIDVSPGMVAAARRRCAGLANVHLAECSGLDLAGFPDARFDLVL
                     AVDSLPYIHQGGPDLVEAHFREVARVLRPGGDFAILNFSYRSDLQADCEEVEELAERH
                     GLFLEAGGFQPFAFWDGIAFLCRRLPELR"
BASE COUNT         2634 a         6225 c         6611 g         2627 t
ORIGIN      
        1 ccccgagacc cggaaggcct acgaccgcgg cctcgacccg aaggcccgcg ccaccctggt
       61 cgccgccatc gctcccaccc aggacgcctc gcgcgacctg gcccggggct actaccagcg
      121 cgcctcctcc ctggtcgacg ccgaggacta tcacttcgcc gtcgagctgc tgcagcaggc
      181 cgtcaacatc gatccgcggg gcgagtactt cgcgctgctg ggcaggatcc aggcccgcaa
      241 cccgcaatgg ctcgaggacg ccgccgccag cctccagcgc gccatcgacc tggggacgcc
      301 cgaccgctcc ctgccggacg ccctcgccga ggtccgccgg ctgatcgcca ccggcgaggc
      361 ctccgaggag gagatggaga tctccgtcgt cgaggtggag gaggaggacg aggcaccgcg
      421 caagccggcg cccaagccca ggacggggag caagcgcggc cggggcaagg cggcggggaa
      481 gccgcgcggc tagaaaagcc gttacactat ccggcgccgg agagctcctg acggtcgctc
      541 cgcacggccc tcgggccggc gcggggagga aagtccgagc tcccacgggt agcatgctcg
      601 ctaacggcga ggcgcggcga cgcgacggaa agtgcaacag aaagtatacc gcctggctct
      661 tcggagcccg gtaagggtga aatggtgcgg taagagcgca ccgctccggc ggcgacggcg
      721 gaggcacgga aaaccccatg cggagcaaga ccaaataggg gagcggtacg cgggccgccc
      781 gtcccgcctc cggttcaggg gctcgtcccc ggggccgggg ccgcttccgg gtaggttgct
      841 ggaggccggc ggcaacgccg gtcctagagg aatgaccgtc ctcggtgctt cggcgccggc
      901 gacagaactc ggcttacgga gctctccggc cctttgattc gatccgcacg cctgccgcct
      961 cccgtgggac ccggtgaccg ggtgggggtc gccgccctct ccgggcccgt cgatcccggg
     1021 aggctcgcgg ccggcatgga cgccctccgg cgcctgggct tcgagccggt gcccgccgac
     1081 aacctcgcct cgcgccacgg cttcttcgcc ggcactgatg ccgagcgcct cgccgccttc
     1141 catcgcctgg cggcggaccc cgaccttccg gccatcctgt tcgcccgcgg cggccacggc
     1201 ctgctccggc tcctccccgc catcgactgg gacctcctgc gccgccgccc tcgcgcctac
     1261 gtcggctact ccgacctcac gccgctcctc ctcggcatcg tcgagcgcct ggggtgggtc
     1321 accttccacg ggcccctcgt ggcgggcgac ctcgcccgcg gcctctcggc cgacgaagag
     1381 gcgagtttcc tcggcgtcct cgccggccgc tatcctgtcg tggagccgct cggccgcgtg
     1441 ctgcgcggga cggcttccgg gaggggggcc gccgccgccg ggccgttcgt cggtggctgc
     1501 ctcagcctgc tggccgcggt gcaagggacc ccctactttc cggatctgga gggggctctg
     1561 ctgttctggg aagatatcaa cgagcccccc tacaagatcg accggatgtt gacccaacta
     1621 ggcctgtccg gtaggctccg ggccatcgag gggatgatcg tcggccatct gggctggacg
     1681 gaggcgccgg tgacgggagg gggggccgag gggggggctg tcggcacgac cgactggccc
     1741 gccgtggtcg cggagagcgt cgccgggttc gactggccgc tcgtctgggg tctcgagagc
     1801 ggccacctgc ggcccaacca tacgctgccc ctcggtctcg gcgcccgttt ggaggcacgg
     1861 ccggatgcgg cggccggcga gatcgtgttc gaagggtgaa tcatggcgaa ccccgtattc
     1921 aagaccaccg gcagcggcgc ggcgaagaag agctccggcg cgaacatcat cgacctcaag
     1981 gcgggggagt acatcttcca ggagggggat ctcggcacgg agatgtacat catccacgag
     2041 gggaaggtcg agatcctgag ccaggcggcc ggcgaggagc agctcctggc ggtgctcgag
     2101 aagggggact tcttcggcga gatgtcggtg ctggaggaca tgccccgggc ggcctcggcg
     2161 cgggcgctca cccactccgt gctcctgcag atcaacggtt ccaccttcga tcagatgctg
     2221 caggcgaacc cggagatcgc ggtgcgcatg atgcgcaagc tgtcgcgccg gctgcgcgag
     2281 acggacgagc tgctccgcgg cctgcagcag ctccagggta cggccatccg ctccgccgcc
     2341 gggggcgcgc gtgagctccc caagccgatg gttcaggtgc cggccgaagg gccggagcgg
     2401 ctggtgcacg ccccctcggg gatggagttt ccgctctcca ccagctcgga gacgaccatc
     2461 ggccgcaagg acccggtcac cggcatctac ccggacgtcg acctcacccc gatcgacggc
     2521 cagcgctcgg tcagccgccg ccactccaag atctaccggc gcgggaccaa gttcttcctc
     2581 gccgaggaga tcggcaccat gaacagcacc tacctgaacg gggcgcggct cgacaccggc
     2641 gtgccggccg agctcaagcc tggcgacgag gtgcgttgcg gtgtggtcat cctccgtttc
     2701 gaggtcccct aacgccatcg ggtagcgcta tcggattgga cctcggcgcc cccctccggc
     2761 gggtcccggg cgtcggcccg gcccgcgcct cggccctgag caacgccggg ctcgtcaccg
     2821 tccgcgacct cctgttccac ctgcccgtcc gctacgagga ccgccgccag gcccggcgtg
     2881 tggcggagct ggttccggac gaggcgacca ccgtgcgcgg gtggctgcgg gacgtccggc
     2941 gcctccgcac ccggcgccgc gggttctcga ttgtccgcag tgtgctcgac gatgggaccg
     3001 gccagatccc ggtggtctgg ttcaaccgtc cctatctggc ggggcaggcg gccggggagg
     3061 gcgaaggcga gtggatcctc cacggcaagg tgcgggaggg caaggggggg ccggagctgc
     3121 tcaacccgag ctgcgagccg gccagggggc cggaggccct gcacgcgggg cagatcgtgc
     3181 cggtctaccc gtccgccgga tcggtgggac ccgcggcgct gcggcggatt ctggacgccg
     3241 tgctcggcga ggtggacctt ccggctcagg tgcaagagac cctgccggcg gagctgctgg
     3301 agagatacca tctgccctct ctcggcgagg cgctggaggc gctgcaccgg cctgggccgg
     3361 acgccgacgc cggtctgctc gagcggcggc gcagcccggc ccacctgcgg ctgatcttcg
     3421 gcgagctcct ggagctgcag ctcgccctcg ccctgctgcg gcgccgggaa ctggccgagc
     3481 cgcggcgcct gcggtatcgg ctggggcggc cgggcgcgcg gctcgagaag aggctccggg
     3541 agctgccgcc gttcccgttg accgccgccc aggagcgggc ggtccgggag atcctcgccg
     3601 acctgcgggc gccccacccc atgctccgcc tgctccaggg ggacgtaggg agcggcaaga
     3661 ccgtggtggc ggccctggcg ctggctgcgg cgctggagaa cggctatcag ggagccttca
     3721 tggcgccgac ggagatgctg gccgagcagc acttcgcgag cctggagcgc ctgctggggg
     3781 gacgctaccg gctggggctc ttcaccggca gccgccgcga cccggccctc ctcgcggcgc
     3841 tggcggccgg cgaggtccag ctcgcggtgg ggacccacgc cctgatccag gatggggtgg
     3901 cgttccagcg gctggccctg gcggtggtgg acgagcagca ccgattcggc gtgatccagc
     3961 gcgggctcct gcggcgcaag ggggagcggc ccgacctgct ggtgataacg gcgacgccca
     4021 tcccgcgctc cctcgccctg gccgcctatg gcgacctgcc ggtctcggtg ctcgatgaga
     4081 tgccgccggg gcggccgccg gtggagacgc gggtggtccc ggcggcgcgg cgccgccaag
     4141 tctacaaggt gctgcgcgag gatctgatgg aaggggggag ggcctacgtg gtcttccccc
     4201 tgatcgagga gagcggccag gtcgccgccg cgtcgctcac cgagatgggg gagcgggtcc
     4261 gccggctcct gtcaggcatc cccagcgcgg tcgtgcatgg gcggatgccg gcggccgagc
     4321 gcgagcgggc catgcgcgcg ttcgccaccg gggaggcacg ggtgctgatc gcgaccacgg
     4381 tggtggaggt cggggtggac gtgcccgagg ccacctggat ggtgatcgag agcgccgagc
     4441 gcttcggcct cgcccagctc caccagctcc gcggccgggt ggggaggggt acgcagccgt
     4501 cacgctgcct tgccctctac ggcccgctat cggacgcggc gcggcggcgg ctcgaggtct
     4561 tccaggagac cacggacggc ttcgtcatcg cccaggcgga cctcgagatg cggggcccag
     4621 gggatatcct cggcacccgc caggcgggtc tgccggcgct gcgggtggcg gatcccgtcc
     4681 gtgacttcga gtggctggag cgggcccggg acgtcgtcgg ggagttgctc gtccgcctgg
     4741 gagagccggg ctggaggatc tcctccaccg cgccggcccc ttgctgccgg agggcggagg
     4801 agccgggtga agagaacgga accatgacca tcaatcatcc ccctgccgag cccctgcgcg
     4861 ttttgctcgt ggccaacacg ctccctcccc gggacgtttc cggcgtcggt gaacaggtgc
     4921 tacagttggc ggccggtctg cgggatgccg gttgtgaggt ggagatcctg ggacgcggcc
     4981 ccggcggggt cgccggcccg aaggtcctgt tccccctctt cgtggttccg gccctccggc
     5041 gggcgctcgc gcggtttcgc ccccacgtgg tgcaggtgca cgagtcggac ggtggcctgg
     5101 cggccctggc ggcgtcggtc gtggcgcccc tgttggagcc ccggcccctg gtcgcggcgc
     5161 tgctccaggt gagctatgtc gaggagctgc gggcggtgcg cccgctggtg agcagcggtg
     5221 ggagggcgtt gggccggccg ggtggggtgg agcgccgctt ccggctgggc aaggcgccgc
     5281 tccagatcct ccttggctgg ctgaccgcct gggtggcgga cgttgtcctg gcccccagcg
     5341 cggccaccgc cggcgagctg cggcgtgatt atggggtcgc ggaggtggag gtggtcccca
     5401 acgtcaccgg cgggttggaa atcgagccgc cgcgggacga gccgggcgag gcgccgagtg
     5461 gctacctcct gttcgtcggc cgactgcgca tccgcaaggg ggtggaggtg cttctcgaag
     5521 ctcttcgcga ctttcccggc gcgcggctgc tgatcgccgg cgatggcgag caccgggggg
     5581 cgctggagcg caaggccgcc gagctggatc tcggccccgc cgccgtcttc ctgggccgct
     5641 gcgacgcctc gcgggtgcgc gggctgctgc ggcacgctgc cgccctggtg gtcccctcga
     5701 tctacgaggg catgccgctg gtggtgctgg aagccatgga ggccggcgtg cccgtggtgg
     5761 ccagccgggt gagcggcatc cccgaggtgg tggaggacgg gcggaccggc tggctggtgg
     5821 agcccgagga cccggcggcg ctggccgcgg cgctggccgc cgtcctgaac gaggacggcg
     5881 aggcgcggcg gcggggcgag gcgggccgcc tccgggtgga ggagcgcttc cggcccgcca
     5941 aggcggccgc gcgctggttg gagctgatga gaccgaagat ccgggagtcc gggagaggaa
     6001 ccctgccgtg aaattgggaa gagccctgct cgctgtgggc gtcatcgcga ccctcggcct
     6061 gatcgccacc gccgtgctcg gctacatgct gcccgggccc gccgacccgg cggtgccgcg
     6121 gcacgtcctg gcggccctgg tggcgagcct gctcctcctc ttctctcatt gctggatcct
     6181 gctctacctg atcgccaccg gccgtgccat ccgtgaggcc gtccgcgagt acggcgtgga
     6241 ggcctccctg gaagccgagg cctggcggct ccggcgtggc gtcctgccct ggctgctgct
     6301 cgccgcggcg acggccctgg gcaccttcct cctgggagct gcagcgatgg ccggcgcggc
     6361 atcggccgac gcccatcaca tcctcttcta tgtcaccctc gtggtgcagg cgctcgccct
     6421 gtggatcgag agccgcgggc tggccgccaa cgagcggctc ctcggcgaca tcgaccagcg
     6481 gttgggcatg gcgcccacac cgccggcacc gccggtatcc gccggagcac ggggagcggc
     6541 atgagcccgg agcgcctgtt caccggcggg gtggagatcc tcgtcctgct cctggccatc
     6601 tccgcccacg agtcggcgca cgcctggacg gcggaccgct gcggcgactc gacggcgcgc
     6661 agcctcggcc gcgtcagcct caacccgctg cgtcacctgg acatcttcgg ctcgctcctc
     6721 ctgccgctgc tccaggtgtc cttccaggcg ccggtcttcg gctgggggcg gccggcgccg
     6781 gtgcagcccc agagcttccg cgacccccgg cgaggagagc tgctggtggc cgccgccggg
     6841 ccgttcgcca acgtgctgct ggcggccgtc gccgtcttcg gcctgctgat cgcggtccac
     6901 gtcctcgggc cggacgcgcg gcgggcggcc tactacaccc tgatgcagca gaccggctcg
     6961 gccgccgacc tgccggggtt cccggtggtc ttcaccctgg tgcggatggc gaccatcaac
     7021 gccttcctgg tggtgttcaa cctgatcccg ctgccccccc tggacggcgg ccagatcgcc
     7081 ctctacatgc tgccgccgga ctgggccgcc aagctggccg gcgtgcggcc gtacggcttt
     7141 atcatcggca tcgctctggc cgcgttcgga gtggtgacgc tgctgctcat ccccttctat
     7201 ggggtgctga gcgtggtcat tcatctgctt tgatcgactc cttgaggatt tatggcgacg
     7261 gaatccaaga aaatcgtcct ctcgggcatg cgcacgacgg gcaagatcca cctggggaac
     7321 tacttcggcg cgctcaagaa ctgggtgggg ctgcaggacc agtaccgctg ctactacatg
     7381 gccgccgact ggcacgccct gaccagcgac tacgcggacc cgtcccaggt gtcgagcaac
     7441 acgctggcga tggtggccga ctggatcgcc gccggcctcg accccgagaa gtcggtgatc
     7501 ttcgtccagt cgatggtgcc ggaccacgcg gagctgcacc tgctcctctc catgatggcg
     7561 ccgctgggct ggctcgagcg ggtgccgacc tacaaggagc agattcagca gctcgagaac
     7621 aaggacctgt cgacctacgg cttcctgggc tatccgctgc tccagacggt ggacatcatc
     7681 atctatcgcg cccacttcgt gccggtgggg gaggaccagg cgagccacat cgagatcagc
     7741 cgcgagctgg ttcgccgctt caaccacctc tacggcggcg gcatctttcc cgagccccag
     7801 gccctgttca ccccgacccc caaggtgccg ggcatcgacg gccgcaagat gtccaagtcc
     7861 tacggcaacg ccatcaatct gtcggacccg ccggacgtca tccgcgacaa gtgcaagcag
     7921 atgttcaccg acccgcagcg gctgacccgc aaggacccgg gccgtcccga ggtctgcaac
     7981 ctctacgagt tccacaagct gatgtcgccg gcagacgtcc aggagcgggt ctaccaccag
     8041 tgccgggccg ccgagatcgg ctgcgtcgac gacaagaagc tcctggcgca gatcatcatc
     8101 gacttcctgg agccgatgca gcggcggcgc cacgagctgg agcgcgaccc taacatgctg
     8161 atggacatcc tcgtcgaggg atcgcggcgc gcgcgggagc ggacggcgga gaccctggac
     8221 atggtgcgcg gagccctggg gctcgactac tctcggctgc gcggggccgt gcccggatca
     8281 gcgggatctg tgggatgagc cggccccgcc gtatcgtgcc gaagccggcg ccgggggccg
     8341 aagcggggga gggagcaccg gtcgctcccg ggatggagac cgccgcctcc ggcagcctgc
     8401 tgcccgaggc gtggcgcgtc cagctcccgg tgttcgaggg gccgctggac ctgctgctcc
     8461 atctcatccg gatcaacaag gtggagatca cggacatccc ggtggcgacc atctgcgacc
     8521 agttccacgc ctacctgggg ctgatggagg agctcaacct cgacgtcgcg gccgagtaca
     8581 tctacgaggc ggcgctgctg atccatctca agtccaagct gctcctgccg cggccggcca
     8641 aggccgcggg cgagcccgaa gaggacccgc gccaggagct ggtggagcgc ctcctcgagt
     8701 accggcgaat gaaagaggtc gcccagtcgt tcgccgaggt ggaccggctg cgcatgggca
     8761 tctggacccg caagccgcag ccgctgcccc aggtggagga agaggaggag caggccatcg
     8821 acatgggcga ggtctccctg ttcgacctcc tctcggcctt gcggcaggcg atggtccgct
     8881 acgacaagga gcacccgccg ccgctgcagc tctcgggcga ggccttctcg gtgcgcgggc
     8941 agttcgaccg gctcctggcg catctcgacg ccgggcaccc gtacgacctc atcaacgacc
     9001 tgcgtgggct ctcctgccgg gcggaggcga tcgccacctt cctggcggtg ctggagctcg
     9061 cccggctcaa cctcatccgt gtccaccaga ccgaggtggg cgacgtcctg ctgtatcgca
     9121 ccacgcgtga gctgtccgat cgagattggg aggagatcgg cggatgacgg agcggcacga
     9181 gatggaatcg gccatcgagg ccatcctctt cgtctcctcg gagccggtgc cccgggccaa
     9241 gctcctggag atgttcgacg aggaggagcg ggagcaggcg aacgaggcgc tgaacgcggt
     9301 gctcgcccgc ttcgaggccg gcgacggccg cggcgtcatg gccgaggacg tcgccggtgg
     9361 cgtgcggctg gtgactcggc ccgaggcggt aggctggctg cgccgcttct tcgacgtcgc
     9421 cggcggctcg aagctgtcga tggcggcgct cgagaccctg gccatcatcg cttaccggca
     9481 gccgatcacc ggtccggaga tccaggagct gcgcggcgtc agctcggcgg gcgtcatcaa
     9541 gaccctgctg gagcgccgca tggtgcgcat cgccggccgc aaggaggtgg tgggcaagcc
     9601 cttcctctac ggcaccacgc gggagttcct ggtccacttc gggctcaaca gcctcaagga
     9661 cctgccgccg ctcgaggagt tcgaggagac cttcggaccc ggcggcgagg ccaccatggc
     9721 attgagcgcc gccgccctcg aggcggtggc gccgccaggg ccggtgccgc tggccggcga
     9781 ggatcgcgag gagcgcatcc tccaggaggt ggcggcgatc gaggaggcgg ccagcgaggc
     9841 gcccgacccg gacgaggggc cgggagagga gtagggtgcg ggtacgcgcc atgcagaggg
     9901 ggaaccggtg atgagcgaag agcggctaca gaagatcctt gcccgggccg gcgtggccgc
     9961 ccgccgcaag gcggaggagc tgatccggga cggcttggtc accatcaacg gccaggtggc
    10021 ggagctgggc tccaaggccg accccgagcg cgacgccatc aaggtggacg gcaagcggat
    10081 ccacccgagc gccgcccacc gctatctgct cctcaacaag cccaaggggg tcatgtccac
    10141 ggtctccgac cccgaggggc ggccgacggt gatagacctg gtgccgcccg gcctgcgcag
    10201 ggccctggtg ccggtggggc ggctggattt cgacaccgag gggctgctcc tgctgactga
    10261 cgacggcgac ttcgcccagc aggtcgccca tccgcgcttc ggctgcacca agacctacga
    10321 ggtcaaggtc aaggggcggc ctgaggagag ccagctcgaa cggctgcgca ccggcatcgt
    10381 gctcgacggc cggcgcacgg cgccctgccg gatcacgccc cacagccgga ccatcaccgc
    10441 cacccgccgc cgcggcaggt tcgacgacgg ggacgacaac actacttggt ggatggtgga
    10501 gctgaacgag ggacggacgc gccagatccg cgagatgttc catcgtatcg gacatccggt
    10561 acagaagctg cgccgggtgg ccatcgggcc ggtgcgcgac ccgcgcctgc cggtaggcgc
    10621 cgtgcgcgag ctctccgagc aggaggtgga gaagctcacc aaggcctcgg gcgggcccaa
    10681 gccggctccg ggagccaaga aggccccggc gaagaaggtg gccgccaaga agactgcggc
    10741 gaagaagggg acggccggga cggaggccgc gaaagcggag aagcggccgc gcggcgagcg
    10801 gcggactctg gccgaggtga gccgggagag ctcccgcgct cccaagggac ccaaggtgac
    10861 ggcggcgggc cgcaaggtga tggcgggcgc gcggaaaggg gcggcgccaa cggccaaagc
    10921 tacaccggcg gccaaggccg ggccgcgcaa gggctccccc cggaccggcg gcggcgcgaa
    10981 ggaccccgcg ggtccgcgct cctcggggcc acgttcgggc catgggcggg gttgagggcg
    11041 tgtccatcgt gcggcctctc atcgtcgcca tcgacggccc ctcgggggtc ggcaagagca
    11101 ccacggcacg gcggctcgcc cgccggctgg gcgtcccctt cctcgacacc ggcgccatgt
    11161 accgggcaat cggcgtgaag gtgctggaga gcggcatcga tcccgccgat cagaaggggg
    11221 tggaggcggt tgcggccgcg gtcgacgtcc agctcgggca cctcgacgac ggcagcttcg
    11281 aggttctcct cgacggccag cccgtggagc accgcatccg cacgcccgag gtcagcgagg
    11341 cgacgtccgc catctccgtc tacccggagg tccgccgccg cctggtcgcc ctgcagcagg
    11401 aggccgcccg gcagttcggg ggtgtcctcg agggccgcga catcggcacc cacgtcttcc
    11461 ccgacacacc ctacaagttc tacctggacg ccgaccccga ggtccgcggc cggcgccgct
    11521 acgacgagtt ggtcgccgcc ggccgccagg tcgtctatgg cgacgtcgtc gaggacatcc
    11581 gccgccgcga ccaccgcgac agcactcgcg ctgattcccc tctgacctgc ctgcccagcc
    11641 acatcttcat cgacgcctcg aacctcaccg tcgacgaggt cgtggccacc atggagcggg
    11701 cgatcgagga gcggggcggg gagggctgag ctggcgccct cgtctcagtg tcggctctcc
    11761 gagcaccgca gagccgcccg gtcgtcgtcc tccagcgacc gcccctcggc gccgaagtag
    11821 ccgagtacat ggacggccgc gggccggaca tcacctcggt gtggtggatg cccagggcgt
    11881 ggccgagctc gtggggagcc gtggtctgga tgtcgaggcc cttgccggtg gtctgccact
    11941 tctgggcggc gttgaaggag atgtccgact cgacgatgac ctgaccggac tggaggacca
    12001 gcgaggtgat ggccagacag gtgcccgtgc agccgttccc cttggcccac gagatggtgt
    12061 tgcggtcgtc cacggcgaag ccggcggtcg tggtgcccgc aagggtcagc acgtgctcgg
    12121 agccggcgcc ggtccagctc ttgaccgccg ccgtcagggc cgccgccgac ttgcgggccg
    12181 cctgtcgatt cgccttcgcc ggtggttcac gaaccactcg atctgctgat tctcccagtg
    12241 tacgaagccg ttggagacat cgcacgccgc cgccgcgctg gcgctcaccc ggcccacgga
    12301 ccggttctgg atcatgcgat aggcctgcga cggaacggcc cagacgagca acacccccac
    12361 cacgaagaca cgaagggcca gacgagagag ggtctcggtc acgatacacg gctcccgcgg
    12421 gcgcgaaggt cgatgctgct cttgcggcca gcttggttgt tgggcctact tctagcccga
    12481 atcaatatga ccgcaagagc gcgggggagc tcaccgtgcc gggttgcgtt cccaggagcc
    12541 gtacatgggg ttggggatca ggatgaagcg ccggccgaag tcggagtagg cggagtcctg
    12601 cttcgccagc aggctctggt cgaggtcggg aaagtccttg atgttgtcgc cgacccagag
    12661 gaggatccgc agcggcggca cccccggcgc ggcggtgccg ttctcgacct tctgccagcg
    12721 cggctccttt tcgctgtcgt tctccatgca gagcatcagg tcgtaaggga tgccgtcgtt
    12781 ccggaagtcg gcctcggtgt ccgggcactc ggcgttgctc cggttggtca cgatggcgat
    12841 cttgccaccg agctcgtgga cgcgggtgag gaaggcgacg gcaccgggca gcggcgtcgc
    12901 cgccttcttc tccgtccact gcttccacga ggcggagtcg aagccggccc cctgcgccgc
    12961 gcgctccttc atgtagtcgg agttgtcgat gaccgtctcg tcgccgtcca gggccacggc
    13021 ccaggtgccg gccgggacgc ccttggccag cttctcgatc ctctcggtgg ccaggaggta
    13081 ggcctggagc agggccgcgt ggtgctcggc ggagtcgcgc acccagtgaa tggcggcggg
    13141 cggcgccgcc ggagctgcct gggacggggc tgtctgggac ggggtcgcgg cgggagccgg
    13201 ctgggccggg ggagccgccg tcggcgtagg ggccgtggca cagccggagg ccaggacgac
    13261 gacaaggatc agggcacggc acgtcatttt catcctcctc agcggttgga ccccgagtct
    13321 ataggaggcg gggggatccc cggcaatcct cttgcagaga acctcgagga aggagcggtg
    13381 tgtgggcgtg tggctgcctc gcttccgtaa gactgacaac cagaaaggag agtcagacat
    13441 gggtctaatc gatttcgcga agagtgtggg ccggaagctg ggcctgggag atgatgacga
    13501 caagaagccg cagggccaga aggtgccggg ccccggcgcg caggccgctc cgaccgcgca
    13561 gcaggtcgag gagctgaagg agcgccgcaa ggccgcggcc ctggtcaagc tggtccagga
    13621 gatgggtttc aaggtgcagg acctaggcat ccgcgtcgac aacgaccgag tcaccctgac
    13681 cggtaccgcg gacacccagg agaaccggga gaagatcgtc ctcctcgtcg gcaacgtcga
    13741 ggggatcggc agcgtcgacg accagctcaa ggtcagcacg cccgcgcctg aggctaccta
    13801 ctatgaggtc aagtcgggcg acaccctgtc caagatctcc aagcagttct acggcgatgc
    13861 caacaagtac atggtgatct tcgaggcgaa caagccgatg ctcaaggatc cggacgagat
    13921 ctaccccggc cagcggctgc gcatcccggc ggccgtggaa tccgccgccg gccgtcactc
    13981 ctgaacggct cggcttcctg ataggagcgg ccccgggttt ccgggggccg ttcctgcttc
    14041 agcgggtgcg gaaggggacg aaccgggagg ccgaaagcgc cgtcccgtcg aaggagcgaa
    14101 ggacgaagcc cgagccctgg gcggtggcgg cacagccgga gagaaggagc cagatctccc
    14161 ccttgggcac cagcacctgg gtgaccggga gctgctcgcc gctcttgctg cgcacctcgc
    14221 tgcgggtctc ggagaggcgg atccagtcgc cgctcggcgc caagcgggtg aggaccacgg
    14281 tctcctcggt tggccggcgc cggtcgaacc agacgagccg cagggcaccc cggtgatcgc
    14341 cgaccggcag gtcgacccag tagtcggtgc cctgcctcca catccccgcc gtgctctggg
    14401 ttccccaggg tgtccaggtg gagcggcgcc agcccttgcc ggagtcgtcc gacagataga
    14461 ccactccatc ggccgagacg taggcggcag aggtgccggt gagggcgatg atctgccccc
    14521 gccgctccgc cggcaggctg ggcacgcccg tcacccagac ctccggccgg cccgagctgg
    14581 agaagagggc ggtgccggtg agcaccccat ccttgcgctg gggcgccggc agcgccgacg
    14641 gctggcccac ggcggtcacc atctggccgc ccacgttctt ccactcgcgg ccctccatcc
    14701 ggaaggccca ggcctggatc tggccgtcct gccggctggc gccgtggagg atcacccacg
    14761 gcgcgccggt gttgtcgtac gtcggagtga ccgtggcggc cgtgggcgag gtcaagggat
    14821 cacccagcgg ctcccacccg ctgtccgagg cgccgccggt ccagcgcata ggctggaggc
    14881 aggaggtgcc gcaggaggtg accgccagca ccggacccgg cccgaccgag ccgccgaaga
    14941 gccccttcgg agggttcggc caagcccggg cctcgcggcc ttcggggtcc agctccacga
    15001 gccagcccct gccgtcctcg cctgtggaag ggatggagaa gagcatccgg ccggacgccg
    15061 tatccattcc gaggagcgtc acggcggtcg agccgtcgca gatcgcgagt ggcgcggccg
    15121 ccgtggggag gctcacgagg agggccaagg cggccaggag cagcggacgc atgaacggca
    15181 cggttttcct ccagagcgcg atcagccgcg acgggcttcg cggatggtcc gtccccagtg
    15241 ttgcgcgcgc atcgatttga ccttggcgat ccgctcctcc gccacccggt cggcggcgac
    15301 gtaggtcggc accgactggt cgcgcgagat ctggaacacc cgcatcatgt tgaggtagat
    15361 gccccgggtc atacgcatcg cccgttcgcg gttgtagccc gtcagctcgt tgtagacgtt
    15421 gatcaggccg ccggcgttga tcacgaagtc gggcgcgtag aggatgcccc ggtcgtggag
    15481 cgcctggccg tgctgggcct cgtcggcgag ctggttgttg gccgcccccg ccaccacctt
    15541 gcagcgcagg gccgggatcg tctggtcgtt gaggatggcc ccgagcgcgc agggcgccac
    15601 gatgtcgcag tcgacggaga ggatctcggc cgtggggacg atctccaccc cgagatcctc
    15661 gttggccttg gccgtggcct cggtgtcgat gtcggcgccg aagaccttgg caccctcgtc
    15721 cttgaggtac ttcgccagat gatagccgac gctgcccagc ccctgaatgg cgacggagcg
    15781 gccggtgagc acgccgtcgt tcccgccgag ctgccagtgc gaggcggcct tgatgccctg
    15841 caggacgccg taggcggtca ccggcgacgg gtcgccgccg ccgccgtggg cgggggagac
    15901 gccggtcacc cagcgggtct cctcgaggac gacctccatg tcctccatgt cggacccgac
    15961 gtcctcggcg gtgatgtaga ggccgcccag cgactcgacg tagcggccga agctgcggaa
    16021 cagagcctcg ctcttgtcct tcttcggatc gccgatgatc acggccttgc cgccgcccag
    16081 gttgagcccg gcggccgcgg ccttgtaagt catgccacgg gacagacgca gcacgtcggt
    16141 gacggcgtcg tccatggact tgtagggcca catccgcact cctcccagcc cgggacccag
    16201 cgcggtcgaa tgaacggcaa tgatggcctc gagccccacc tcggggttgg agcagacgag
    16261 cacccgctcg tgccccatgc tcgccatctg ctggaagaga tccatgaacc tccctttctc
    16321 aggagacgat gtcgttggaa acacgccgca cccaggggaa gccgtcccgt gtggatggcc
    16381 acacgaatac cgtgtgggcg cgaacggccg gaagtgtact cgaaggggtc gcgagggatg
    16441 aagccgcgga gcggcgcggg cgtgccgcgg tgcgacgccc ggcgcccccc ggcgccctca
    16501 gagcttcccg agggctgcca gaacctcctc gtcgtgcccg tcgacgctga cccgcttcca
    16561 gaccttgcgc accttgccgt cggcgtcgat gaggaaggta gagcgctgga tgcccatcga
    16621 ggtcttgccg tacatgttct tctcccgcca ggcgccgtac tgctcggcca cctgatggcc
    16681 ggtgtcggcg aggagcggga agttgaggct gtacttgtcg cggaaccggc cgtggctcgc
    16741 cacgtcgtcg ggagagacgc cgaggacgac ggcgccgcgg tcggcgaggt cctgccggcg
    16801 gtcccggaag gcgcacgcct ccttggtgca gccgggcgtg tcgtcctgcg ggtagaaata
    16861 cagcaccacg ggcttgccgc ggagttccga gagcttcacc tggcggccgt cgtcggcggc
    16921 gagggtgaaa tcgggcgcgg tctgcccttc ttcgatccaa tcggacatgc tttcgtctcc
    16981 tcggagaaaa agtcacggcc gggcgggccg cccgggccag ggaagtgaag tctaccgcag
    17041 ctccggcaga cggcggcaga ggaaggcgat gccgtcccag aaggcgaagg gctggaagcc
    17101 ccccgcttcc aggaagagcc cgtgccgctc cgcgagctcc tcgacctcct cgcagtcggc
    17161 ctggaggtcg gagcgatagg agaagttgag gatggcgaag tcgccgccgg ggcgcagcac
    17221 gcgggccacc tcccggaagt gggcctccac caggtcgggc cctccctgat ggatgtaagg
    17281 aagggagtcg acggcgagca cgaggtcgaa gcgggcgtcg gggaagccgg cgaggtcgag
    17341 gcccgagcac tcggcgaggt ggacgttggc gagcccggcg caccgcctcc gggcggcggc
    17401 gaccatgccg ggggagacgt cgatgccgtg gatctcggcc agccggggag cgagcgccgc
    17461 caggacgcgg ccgatgccgc acccgatctc cagggccgag cggtcgggat cgagcaggcc
    17521 ccagcggtcg aggaggacga ggatctcctc cgtcgcctgc tccagcagct ctgggctgcc
    17581 cagggagtag gcggcgacgc tcgccacctc gtcctgccgc accgcccggt cgaagaagcg
    17641 gcggatctcg tcgacggctg tcccaggagg cgaggggggg gcgggagcgg ggtggtggcg
    17701 cagcatcgag gtgatgcgct cgcaggcgtc gcgatggtcg aggagcaggc cctccagctc
    17761 ggccgccacc ggatgcccgg tcgccaccgc ctccccgatc aggtccgcca ccgcctcgac
    17821 gctccccagc tcgtcgagca gccgcacgag cccgagcgcc gggtgggtgt cgcccgtcag
    17881 gcatccgttc aggatgtgcc gcgacgccga ggagggggcg gcgatcttgg gggtgtcccg
    17941 cttcggcatg ggaggaatgg tacaaccccg ggggtatccg ccctggtatc cgccctcacc
    18001 cccgggtgct gtcttcatca ggccatgaac cccaggtccg ggttgtcgaa gcgcccgagg
    18061 ttggggaaga ggagggggag gtcggcgttg ctcactc
//
LOCUS       DASPJI010000390         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_21603, whole genome shotgun sequence.
ACCESSION   DASPJI010000390 DASPJI010000000
VERSION     DASPJI010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21603"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VEL74_10380"
     CDS             <1..552
                     /locus_tag="VEL74_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62977.1"
                     /translation="SPSNTELILKVLDFGDVVKIFYGQLTNLRFTLIVTDTQTGTSRV
                     YGNTPGECGAIDQSAFAGSGAAPISAAKAGPCRPGPGTLCLMGNRFEVTAEWRNPGDG
                     SSGQAGAVPLSALTGAFWFGDRNNLEVMTKILDLGGRIDFFYGTLSDLEYTLTVTDTA
                     TGTVKTYRNPAGTYCGGLDDAAF"
     gene            complement(603..830)
                     /locus_tag="VEL74_10385"
     CDS             complement(603..830)
                     /locus_tag="VEL74_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62978.1"
                     /translation="MSWNRREFLGRSLMGVGLACLPLAGVGAAPAAASPAPALPDFGA
                     TAAPGMERWLLDTGRSHIDAPIYDLVSSRIR"
     gene            complement(860..1720)
                     /locus_tag="VEL74_10390"
     CDS             complement(860..1720)
                     /locus_tag="VEL74_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62979.1"
                     /translation="MLRIELDIFSGRPNPSWILSPKEERELVDRVKADPSLMLPVTAA
                     TGGLGYRGYIVTREKEGEESQGKAALPSSFRLGGEPLDKDAATALWLLDTSEKADTEV
                     DDFLRGAAAETIRQPPARLGKLAPGQGPEAQDKGSLVACASNYLTSSTDFSFWNGSTY
                     IRKNNCYNFAANHRTNTFAQPGRISGHPITGLTCSAVASSAEWDGWFNGCQAANNLTI
                     ALVIWPGVDFHFYRKVANGVWGHKPGQTAARNYDNSGVVITNPQTCNRGNYTSFCGYW
                     YADNNIIMVQ"
     gene            1917..>2289
                     /locus_tag="VEL74_10395"
     CDS             1917..>2289
                     /locus_tag="VEL74_10395"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYG62980.1"
                     /translation="MRRNDTLLDLFLELQILDRIPRSGYTLRGVADPESVTEHSWHVV
                     FLVWTLGARIEGLDVQRAVEIALVHDLAELRVGDLPRTASHYFPEGAKKSAEMAAMQD
                     VLYPLPERSMGLYREYQEGTSP"
BASE COUNT          359 a          786 c          763 g          381 t
ORIGIN      
        1 tcgccgtcca acaccgagct gatcctcaag gtcctggact tcggcgacgt ggtgaagatc
       61 ttctacggcc agctcaccaa cctgcggttc accctaatcg tcaccgacac gcagaccggc
      121 acatcccggg tctacggcaa caccccgggc gagtgcggcg ccatcgacca gtcggccttc
      181 gccggctccg gcgctgctcc catctcggcc gccaaggcag gcccctgccg ccccggcccc
      241 gggaccctct gcctcatggg caaccgcttc gaggtgaccg ccgagtggcg caacccgggg
      301 gacggctcga gcggccaggc cggcgcagtc ccgctgtcgg ccctcaccgg cgccttctgg
      361 ttcggcgacc gcaacaacct cgaggtgatg accaagatcc tcgacctggg cggccggatc
      421 gatttcttct acggcaccct gtcggacctc gagtacaccc tcacggtgac cgacaccgcc
      481 acggggacgg tcaagaccta ccgcaacccc gccggcacct attgcggcgg gctcgacgac
      541 gcggcgttct aggatgggat ggggggcggc ccctctcggg agagaggagc cgccagggcg
      601 gactagcgga tgcggctcga caccagatcg tagatcgggg cgtcgatatg ggaacgcccg
      661 gtgtccagca gccagcgctc catgccgggg gcggcggtgg caccgaagtc gggcagcgcc
      721 ggggccgggc tcgcggcggc cggcgccgcg ccgacacccg ccagcggcag gcaggcgaga
      781 ccgacgccca tcagggagcg gccgaggaat tcacgtcgat tccaactcat ctgtcgttcc
      841 tcctgggttc agccgttctc tactggacca tgatgatgtt gttgtcggca taccagtacc
      901 cgcagaagct ggtgtagttc ccacggttgc aggtctgcgg gttggtgatg acgacaccgg
      961 agttgtcgta gttgcgcgcg gcggtctggc cgggcttgtg gccccagacg ccattggcga
     1021 ccttgcggta gaagtggaag tcgacgcccg gccagatcac cagcgcgatg gtcaggttgt
     1081 tggccgcctg gcagccgttg aaccagccgt cccactcggc ggaggaggcg acggcggagc
     1141 aagtgagacc cgtgatcggg tggcccgaga tgcgtccggg ctgggcgaac gtattggtgc
     1201 ggtggttcgc cgcgaagttg tagcaattgt tcttgcggat gtaggtggag ccgttccaga
     1261 agctgaaatc cgtcgagctc gtgaggtagt tcgacgcgca ggcgacgagg cttcccttgt
     1321 cctgggcctc ggggccctgc cccggcgcaa gcttccccag acgtgcggga ggctgccgga
     1381 tggtctcggc ggccgcaccc cgcaggaaat cgtcgacctc cgtgtccgcc ttctccgagg
     1441 tgtccaggag ccagagggct gtcgcggcgt ccttgtccag cggttcgccg ccgagccgga
     1501 agctggaggg cagggcggcc ttgccctggc tctcctcccc ctccttctca cgggtgacga
     1561 tgtatccccg gtagccgagc ccgccggtgg cggcggtgac cggcagcatg agcgaagggt
     1621 ccgccttgac gcggtccacc agctcacgct cctccttggg agacaggatc caggacgggt
     1681 tgggacggcc tgagaagatg tcgagctcga tccgcagcat gaatacactc ctcctgtaga
     1741 agttcatggg gccgacgcct ggaactgcac gaaggcctcg acaatattct tcggtgcgca
     1801 tagcctctcc tggcgagccg ccccgccggg tccacagtga ccggacctct ctcgccggca
     1861 cttgttccca ctttccgtct cgacgctccc ctctctttgc agtaccctcc cccaccatgc
     1921 gccgcaacga cacccttctc gacctcttcc tcgagctgca gatcctcgac cgcatccccc
     1981 gctccggcta tacgctgcgc ggcgtcgccg acccggagag cgtgacggaa cacagttggc
     2041 acgtcgtctt cctggtgtgg acgctgggcg cgcggatcga agggctggac gtccagcggg
     2101 cggtggagat cgcgctggtc cacgacttgg cggagctgcg ggtgggagac ctcccccgga
     2161 ccgcctcgca ctacttcccc gagggggcca aaaagtccgc ggagatggcc gccatgcagg
     2221 acgtgctgta cccgctgccg gagcgctcca tgggcctcta ccgggagtac caggagggca
     2281 ccagccccg
//
LOCUS       DASPJI010000391         7588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_536956, whole genome shotgun sequence.
ACCESSION   DASPJI010000391 DASPJI010000000
VERSION     DASPJI010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7588)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536956"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VEL74_10400"
     CDS             <1..749
                     /locus_tag="VEL74_10400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HYG62981.1"
                     /translation="AYLDVTDHLGGHGSATALAREIRRRVREERGLTVSVGVGPNRLV
                     AKIASDFHKPDGLTVVPPGKVQAFLDPLPVRRLHGVGPATERALGELGVQTVAELRAV
                     PLDVLSGRFGKHGRILFEFACGIDERPVETHQERKSLGTENTYAVDLVGLARIEEEID
                     KMAEEVAAGLERRSLAGCTVTLKVRYSDFTTVTRSRTFNIPVRDRTTIAGCAKDLLRK
                     TDAASRPVRLIGVTASTLVHGLIEQLRLFE"
     gene            793..1173
                     /locus_tag="VEL74_10405"
     CDS             793..1173
                     /locus_tag="VEL74_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYG62982.1"
                     /translation="MEAAALSPREYHARRERERQDERESLRQDVLERVRQSIRTHAPR
                     FPAIRAVYLFGSLVQPGRFTSGSDVDLAVDVDDIQVETPFWRALEEELERNVDLRPRA
                     GAVARAVESYGELCYEREVGPPGK"
     gene            1142..1609
                     /locus_tag="VEL74_10410"
     CDS             1142..1609
                     /locus_tag="VEL74_10410"
                     /inference="COORDINATES: protein motif:HMM:NF044627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HYG62983.1"
                     /translation="MSAKWVLLESSIRDDLRSIERLYEDLGHPRLDGSETEEERIVVA
                     YRLHNLYSAFENIFRNIAAAFENRLDERGGWHRELLRRMRLDLQPVRPALIDDETYEK
                     LDEMLRFRHLFRAAYSVSFDPARLRLVLDKALDLRPLYPPQVERFLEFLHLLE"
     gene            1632..2660
                     /locus_tag="VEL74_10415"
     CDS             1632..2660
                     /locus_tag="VEL74_10415"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HYG62984.1"
                     /translation="MPSSSSPLLRIDDHGLFCEAGGFHIDPWKPVERAVISHGHGDHA
                     RPGSRRYLAPSPSLPILEKRLGGGARIETAPFGETVRVRDVTVSFHPAGHILGSAQVR
                     VERGGEVWVFSGDYKRAADPSCEPFEPVRCHTFITEATFGLPIYVWDDTAAVAREILE
                     WWEGNRALDRTSVLFCYALGKAQRILAELARLTDRPVWVHGAIETLTRCYRQAGVPML
                     PTRRVGDTVKGQPFAGELVLAPPAAGGSLWMRRFGRASTGFASGWMRVRGPRRRRGCD
                     RGFVISDHADWPDLLRTIADSGASRVLTTHGSAGPLARYLCEQGVEASPLATPFEGEE
                     GVESGGET"
     gene            2657..3715
                     /locus_tag="VEL74_10420"
     CDS             2657..3715
                     /locus_tag="VEL74_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62985.1"
                     /translation="MRRFAALIEVLDRTASVNEKAAALAGYFRTAPPADGAWALYFLT
                     GRRLERRLPARLLAGWAMAAAGVADWLFEECYGTVGDLVETIVLVLDTLPRSPGDGAD
                     AALSLSAWIEERVLPLKELPAEEQRTRLLAWWAALDRGQAFVLTKILTGELKAGVSKT
                     VLERALGEVAGLPPATMAARLEGGWEPTAASLRQLLSAGDSSGDGGGTAPSVPPREET
                     VEEPKERPYSVDAVLVYARPGQGWRASHFTDYTFAVWSGAELVPFTKADAGLSETEVV
                     ELDRWIRRHTLQRFGPVRAVEPVHVFEVAFEGIAASSRHRSGVAVRSPRIARWRTDKA
                     AAEADTLERVQALVESPA"
     gene            3781..3945
                     /locus_tag="VEL74_10425"
     CDS             3781..3945
                     /locus_tag="VEL74_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010120894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HYG62986.1"
                     /translation="MLRWALIFLIVALVAAVFGFGGIAAGAADVARILFYLCLAVFAV
                     SLGMGLRRRA"
     gene            4038..5168
                     /locus_tag="VEL74_10430"
     CDS             4038..5168
                     /locus_tag="VEL74_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HYG62987.1"
                     /translation="MLETLQREPADSGEERPRPNIKKLRELLTGPFDVRSLALTGLLV
                     LATFYTLYLARSFFLPIILAVLLSFLLTPVVRLLRKLRVPQGMAAGLVVFGLLGGLGW
                     GVYELSGPAYEWAQKAPQSLRRLERKLRDFKRPVQTMSKATEQVEKITQMGGGASPRT
                     VAVQTETLGERMFSQATELVAGATVMFILLFFLLASGDLFLRKLIRVLPRLSDRKRAV
                     EIARQIESDISAYLSTITVINIGLGLAVWGLMALIGVPNPLLWGVVATVTNYIPYLGA
                     IIMIVVLAGVGFLTFPDLAHSLMPPAAFIGLNILESYIVTPLVLGRRLTLNPVVIFLG
                     LTFWGWLWGITGALLAVPIMVVFKIFCDHSESLASIGEFLGD"
     gene            complement(5404..6414)
                     /locus_tag="VEL74_10435"
     CDS             complement(5404..6414)
                     /locus_tag="VEL74_10435"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYG62988.1"
                     /translation="MPASTLPRHPIGVVARRTGLKPDLIRAWERRYGAVEPGRSDTRR
                     RFYSDADIERLLLLRRVVGTGRGIGQVAGLPNAQLEELLAQEPGRAEPAAQAQGTVLP
                     APPAMPLVGPPGDGAEPFLTLCLSAAQRLDVRDLEMQLERASVALSRPQLMEKLLVPL
                     MHRIGDLWQQGALRPVHEHMASAVVRSFLGGLRSAGGSEATAPHLVATTPARQLHELG
                     ALIAAATAASEGWQITYLGPDLPPEEIAAAALQKGARAVALSLTYPPDDPMLMDELRR
                     LRRLLPSGTEIVVGGRAAPGYHALLQEIGARAVRDMEELRRELRALRAGPMPLTEETG
                     VP"
     gene            complement(6666..7055)
                     /locus_tag="VEL74_10440"
     CDS             complement(6666..7055)
                     /locus_tag="VEL74_10440"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HYG62989.1"
                     /translation="MSVRLDKWLQVARMFKSRTQATHACDLGRVKVNGQPAKPHRQLA
                     LGDRVEVTQGDWDRVLIVQQLADKPVPKAEAATLYEDQSPPRPAADPLARLLRRPPVL
                     RESGAGRPTKKERRDIERWMDGEVEEP"
     gene            complement(7065..>7588)
                     /locus_tag="VEL74_10445"
     CDS             complement(7065..>7588)
                     /locus_tag="VEL74_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62990.1"
                     /translation="EISLASPWNVLFAPVHGLFLFHPALALAAAGLLWMAGRELARRS
                     PGWGTLGAVWFLTVAVLHGWWSEWANTGGYGQRFLIDALPALALGFAAWPRAEPRDRG
                     AWRWGPALAAALFGYLLFLGAVTELAPAPEPYPWPQRLGDYAALVRHPPGPGAIREGL
                     GEASFLLRPLLSR"
BASE COUNT         1008 a         2706 c         2698 g         1176 t
ORIGIN      
        1 aggcctacct cgacgtcacc gaccacctgg gggggcacgg cagcgccacg gcgctggcgc
       61 gggagatccg gcggcgggtg cgggaggagc ggggcctcac ggtgagcgtc ggcgtcggcc
      121 ccaaccggct ggtcgccaag atcgcctcgg actttcacaa gccggacggc ctgacggtgg
      181 tgccccccgg caaggtccag gccttcctcg atcctctccc cgtgcgccgg ctccacggcg
      241 tgggccccgc caccgagcgg gcgctggggg agctgggggt ccagaccgtg gccgagctgc
      301 gggcggtccc tctggacgtc ctctccggcc gcttcggcaa gcacggccgc atcctcttcg
      361 agttcgcctg cggcatcgac gagcggccgg tggagacgca tcaggagagg aagagcctcg
      421 gcaccgagaa cacctacgcg gtcgacctgg tggggctcgc ccgcatcgag gaggagatcg
      481 acaagatggc ggaggaggtg gccgccgggc tggagcgccg gagcctcgcc ggctgcaccg
      541 tcacgctcaa ggtccgctat tcggacttca ccaccgtcac ccgctcgcgg accttcaaca
      601 tcccggtccg cgaccggacg accatcgccg gctgcgccaa ggacctgctg cgcaagaccg
      661 acgccgcctc gcgcccggtc cgcctgatcg gcgtcaccgc ctccacccta gtccacggcc
      721 tgatcgagca gctccgtctg ttcgagtgag gggcagcccg ggcccgtacg gctgagctac
      781 actggcccca ccatggaggc ggctgctctc tccccgcggg agtaccatgc ccggcgcgaa
      841 cgggagcgtc aggacgaaag ggagtctctg cgccaggacg ttttggagcg ggtgcggcag
      901 tccatacgga cgcacgcccc ccgttttccc gcgatccggg ccgtctacct cttcggctcc
      961 ctcgtccaac cggggcggtt cacctcgggc tcggatgtgg acttggccgt cgacgtcgat
     1021 gacatccagg tggagacccc attctggcgc gccctggaag aggagctgga gaggaacgtc
     1081 gacctgaggc cccgtgccgg cgccgtggcg cgggcggtgg aaagctatgg ggagctctgc
     1141 tatgagcgcg aagtgggtcc tcctggaaag tagcattcgc gacgacctcc ggtccatcga
     1201 gaggctctac gaggatctcg gtcaccctcg gctcgacggc tccgagaccg aggaggagcg
     1261 gatcgtcgtc gcctacaggc tgcacaatct ctacagcgct ttcgagaaca tcttccggaa
     1321 cattgccgcg gccttcgaga acaggttgga cgagcgcggc gggtggcatc gcgagctcct
     1381 gcggcggatg cgtctggatc tccagcccgt gcgcccagcg ctgatcgacg acgagaccta
     1441 cgaaaagctg gatgagatgc tccggttccg gcatctcttc cgggctgcct attccgtctc
     1501 cttcgacccg gcgcggctcc ggctcgtcct ggacaaggca ttggatctcc ggccgctgta
     1561 cccgcctcag gtcgagcggt tcctcgagtt cctgcacctg ttggagtagg tcgagggcca
     1621 gcacctgctc catgccgtcc tcgtcctccc ccctgctgcg catcgacgac cacggcctct
     1681 tctgcgaggc cggcgggttc cacatcgatc cgtggaagcc ggtcgagcgt gcggtgatct
     1741 cccacggcca cggcgaccac gcgcggccgg ggagccggcg ctacctggcg ccgtcgccgt
     1801 cgctgcccat cctcgagaaa cggctggggg gaggggcacg gatcgagacg gcgccgttcg
     1861 gggagacggt gcgggtgcgg gacgtcaccg tgtcgttcca ccccgcgggg cacatcctgg
     1921 gctcggccca ggtgcgggtg gagcgggggg gcgaggtctg ggtcttctcg ggggactaca
     1981 agcgcgccgc cgatccctcg tgcgagccgt tcgagccggt gcgctgccat accttcatca
     2041 ctgaggccac cttcggcctg ccgatctacg tctgggacga caccgccgcg gtggcgcggg
     2101 agatcctgga gtggtgggag gggaaccggg cgctggaccg cacgtccgtg ctcttctgct
     2161 atgcactcgg caaggcgcag cgcatcctcg ccgagctggc ccggctcacc gaccgcccgg
     2221 tctgggtcca cggcgccatc gagaccctga cccggtgcta ccggcaggcg ggtgtaccca
     2281 tgctccccac ccgacgggtg ggcgatacgg tcaaggggca gccgttcgcc ggcgagctgg
     2341 tgctggcgcc tccggccgcc ggcggctctc tctggatgcg ccgcttcggc cgcgcctcca
     2401 ccggcttcgc ctccggctgg atgcgggtgc gggggccccg gcggcggcgc ggctgcgacc
     2461 gcggcttcgt catctccgat cacgccgact ggccggacct gctgcgcacc atcgccgaca
     2521 gcggagcctc gcgggtgctc accacccacg gctctgccgg acccctggcc cgctacctgt
     2581 gcgaacaggg tgtcgaggcc agcccactcg ccaccccctt cgagggggag gagggggtgg
     2641 agtccggggg cgagacgtga ggcgcttcgc cgccctgatc gaggtgctgg accggaccgc
     2701 ctcggtcaac gagaaggcgg cggccctggc cggctatttc cgaaccgcgc ctccggccga
     2761 cggcgcgtgg gcgctctact tcctcaccgg ccgccggctc gagcggcggc tgccggcccg
     2821 gctcctcgcc ggctgggcca tggcggcggc gggcgtcgcg gactggctgt tcgaggagtg
     2881 ctacggcacg gtgggggacc tcgtggagac gatcgtcctg gtgctggaca ccctgccgcg
     2941 ttctccgggc gacggcgccg acgcggcgct ctccctctcc gcctggatcg aggagcgggt
     3001 cctgcctctc aaggagctgc cggcggagga gcagcgtacc cgccttctcg cctggtgggc
     3061 ggccctcgac cgcggacagg ccttcgtcct caccaagatc ctcaccggcg agctgaaggc
     3121 gggcgtctcc aagacggtgt tggagagggc cctcggcgag gtggcggggc tccccccagc
     3181 cacgatggcc gcccgtctcg agggagggtg ggagcccacc gcggcgtccc tgcgacagct
     3241 cctctccgcc ggcgattctt ccggcgatgg cggcgggacc gccccctccg tgcccccccg
     3301 ggaggagacg gtggaggagc cgaaggagcg cccctacagc gtcgacgccg tcctcgtcta
     3361 tgcccggccc gggcaggggt ggcgcgccag ccatttcacc gactacacct tcgccgtctg
     3421 gtccggtgcc gagctggtcc ccttcaccaa ggcggacgcg ggcctctcgg aaaccgaggt
     3481 ggtggagctc gaccgctgga tccgccggca caccctgcag cggttcgggc ccgtacgcgc
     3541 cgtcgagccc gtgcatgtct tcgaggtcgc cttcgagggg atcgccgcct cgtcccgcca
     3601 ccgctcgggc gtcgccgtcc gctccccccg cattgcccgc tggcgcaccg acaaggccgc
     3661 cgccgaggcc gacaccctgg agcgggtcca ggcgctcgtc gaatctcctg cctgagcgca
     3721 gggcatggat tttgcttaaa ttcatgacca caagctgttg tcttgcgagg agaacgggtc
     3781 atgctgaggt gggcgctcat ctttctgatc gtcgctctgg ttgccgcggt cttcggcttc
     3841 ggcggcatcg ccgccggcgc ggcggacgtc gcgaggattc tgttctatct gtgtctggca
     3901 gtgtttgccg tctccctggg gatggggctg aggcggcggg cctgaggggg cgtttggccc
     3961 tacaatcgcg gaggcagtga gccgccgccg ctcccgggcg tggtacgcgg accctgtcgt
     4021 tcttacaagg actgcgtatg ctcgagaccc tccagcggga gcccgccgac agcggcgagg
     4081 agcggccccg gcccaacatc aagaagctcc gcgagctgct gacggggccg ttcgacgtcc
     4141 ggtcgctggc gctcaccggc ctgctggtcc tggcgacttt ctacacgctc tacctggccc
     4201 gctctttctt tttgcccatc atcctggcgg tgctgctcag cttcctgctg acgccggtgg
     4261 tgcgcctgct ccgcaagctc cgcgtccccc agggcatggc ggcggggctg gtggtcttcg
     4321 gtctgctggg ggggctgggc tggggggtgt acgagctgtc cgggcccgcc tacgagtggg
     4381 cgcagaaggc gccccagagc ctgcggcggc tcgagaggaa gctgcgggac ttcaagcggc
     4441 cggtgcagac catgagcaag gccaccgagc aggtggagaa gatcacccag atggggggcg
     4501 gggcctcccc ccgcacggtg gcggtgcaga cggagaccct gggggagagg atgttcagcc
     4561 aggccaccga gctggtcgcc ggggccaccg tgatgttcat cctcctcttc ttcctgctcg
     4621 cttcggggga cctcttcctg cgcaagctga tccgggtcct gccgcggctg tcggaccgca
     4681 agcgggcggt cgagatcgcc cgccagatcg agtcggacat ctcggcctac ctgtccacca
     4741 tcaccgtgat caacatcggc ctggggctcg ccgtctgggg cctcatggcc ctgatcggcg
     4801 tccccaaccc gctgctctgg ggggtggtgg cgacggtgac caactacatc ccctacctcg
     4861 gggcgatcat catgatcgtg gtgctggccg gggtcggctt cctcaccttc cccgacctgg
     4921 cccacagcct gatgccgccg gcggcgttca tcgggctgaa catcctggag agctatatcg
     4981 tgacgccgct ggtcctcggg cgccgcctga cgctcaaccc ggtggtgatc ttcctcggcc
     5041 tcaccttctg gggctggctc tgggggatca ccggggcgct gctcgccgtg ccgatcatgg
     5101 tcgtcttcaa gatcttctgc gaccacagcg agtccctcgc ctcgatcggc gagttcctgg
     5161 gcgactgacc cgctgttgtc caggcgttgt caaggttctt ccggcacggg tggatagaca
     5221 gaggcgcccc ccccgtctgg gtgagcgcct ggtgatcgcc gccgtcctct tcacccggag
     5281 aaggatcgcc ccgccccccc gtctcgcccc tcgctcgcgc tctcgccccc gctccccctc
     5341 tccctccccg ctctctcgct ccctcccccg atccttcgcc gggttgaaaa ggacggcgcc
     5401 gtttcagggc acgcccgtct cctctgtcag gggcatcggg cccgcgcgga gggcccgcag
     5461 ctcccgccgc agctcttcca tgtcccggac ggcgcgggct ccgatctcct ggagcagggc
     5521 gtggtagccc ggcgcggccc tgccgcccac cacgatctcg gtccccgagg ggagcagccg
     5581 gcggagccgc cggagctcgt ccatgagcat ggggtcgtcg ggcgggtagg tgaggctcag
     5641 ggccaccgcc cgcgccccct tctgaagggc ggcggcggcg atctcctcgg ggggcaggtc
     5701 cgggccgaga taggtgatct gccaaccctc cgaggcggcc gtggcggcgg cgatcagcgc
     5761 tcccagctcg tggagctgcc gcgccggcgt ggtcgccacc aggtgcggcg ccgtggcctc
     5821 ggagcctccg gcgctccgca ggccccccag gaaagagcgg accacggccg aggccatgtg
     5881 ctcgtgcacg ggccgcaggg ccccctgctg ccagaggtcg ccgatgcggt gcatgagggg
     5941 caccagcagc ttctccatga gctggggacg gctgagggcg accgacgcgc gctcgagctg
     6001 catctccagg tcccgcacgt ccaggcgctg cgccgccgac aggcagaggg tgaggaacgg
     6061 ctccgcgccg tcccccggcg ggcccaccag cggcatcgcc ggtggcgccg gtagtaccgt
     6121 gccctgcgcc tgggcggccg gctcggccct ccccggctcc tgcgcgagca gctcctcgag
     6181 ctgggcgttc ggcaggccgg cgacctggcc gatcccccgg cccgtcccca ccacccgccg
     6241 cagcagcagc agccgctcga tgtccgcgtc cgagtagaag cgccggcgcg tgtccgagcg
     6301 ccccggctcc actgcgccgt agcgcctctc ccaggcccgg atgaggtcgg gcttgaggcc
     6361 ggtgcggcgg gcgaccacgc cgatcgggtg ccgggggagg gtcgaggcag gcatggggtc
     6421 cgtggccatt gggcgagagg ctaacaaagc cgtctacggc ttgtcaagga agaagagaga
     6481 atatttgtgg acaacatatt gacaggtggt ctgcgtttct ctattctcag ctttgcaagg
     6541 acgttgcggc cacccggccc aacgcctctc cagggggggc cgcgacggtc cggcagacgc
     6601 agcaagggac ccaacccgcg gctgcgggtt ctcctccagt gggccgatcg gcccactctt
     6661 tttatctacg gctcctcgac ctccccgtcc atccagcgct cgatgtcacg ccgctccttc
     6721 ttggtggggc ggccggcgcc ggactcgcgc agcacggggg ggcggcggag gaggcgggcg
     6781 agcggatcgg cggcggggcg gggcgggctc tggtcctcgt agagggtggc ggcctcggcc
     6841 ttggggaccg gcttgtcggc gagctgctgg acgatcagca cccggtccca gtcgccctgg
     6901 gtcacctcga cccggtcgcc gagcgcgagc tggcggtgcg gcttcgcggg ctggccgttg
     6961 accttcaccc gccccaggtc gcaggcgtgg gtggcctggg tgcggctctt gaacatgcgg
     7021 gccacctgga gccacttgtc gaggcgtacg gacatgccgg gagtctatcg cgacaggagc
     7081 ggccgcagca ggaacgaggc ctcgccgagc ccctcccgga tcgcgccggg tcccggcgga
     7141 tggcgcacca gcgccgcgta gtcgccgagg cgctgcggcc aagggtacgg ctcgggcgcc
     7201 ggcgccagct ccgtcaccgc tcccaggaag aggagatacc cgaagagggc cgccgccagt
     7261 gccggccccc accgccaggc cccgcggtcc cgaggctcgg cccgcggcca ggcggcgaat
     7321 cccagcgcca gcgcgggaag ggcgtcgatg aggaaccgtt gcccatagcc gccggtgttg
     7381 gcccactcgg accaccagcc gtgcaggacg gcgaccgtca ggaaccagac ggcgccgagg
     7441 gtgccccatc ccgggctccg gcgggcgagc tcgcgcccgg ccatccagag gaggcccgcc
     7501 gccgccaggg cgagggcagg atgaaaaaga aagaggccgt ggaccggcgc gaagagcacg
     7561 ttccagggcg aggcgaggga gatctccc
//
LOCUS       DASPJI010000392         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_82994, whole genome shotgun sequence.
ACCESSION   DASPJI010000392 DASPJI010000000
VERSION     DASPJI010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82994"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VEL74_10450"
     CDS             complement(<1..540)
                     /locus_tag="VEL74_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYG62991.1"
                     /translation="MRRLLPCLLLVLIAAAVPLAAAEPLKVATLLPYIEEALRAAAPG
                     RARVVAGVRRTEGGALAQGVSDLGSPHAPNFERLAASGAEVVVVDERIHKALEPRLRA
                     VGVEVLSVRGDSVEGTFAGIQTAARRAGADREMAARVQEARKALQRLALPRPVPVLVL
                     FGAPGSFLVVTSDTWLGDLL"
     gene            complement(540..965)
                     /locus_tag="VEL74_10455"
     CDS             complement(540..965)
                     /locus_tag="VEL74_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62992.1"
                     /translation="MSTMTSHGNVGTSWTARRFFDALAALGPLRVISQSGPSTFEAIC
                     EVGAFGIAQGYVNAITASYHWHLKLDGFRHLRSRDEIHQRSGRRVLFFELRTEEDAAP
                     FLLVYLHREKGEELAPGREGLFAELHQELAAGLPVEEVG"
     gene            complement(981..>2455)
                     /locus_tag="VEL74_10460"
     CDS             complement(981..>2455)
                     /locus_tag="VEL74_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYG62993.1"
                     /translation="AKLAFIPGGSSMWTLSAEVLRAEAETEIYSARGVTTPGTTTTDA
                     DAVDERRRTRVSLSQSLEATSPLFDRAEWRLHAQEDDTEQRTVDELTSAAGGIARRGL
                     FTFEQDGLGGEVELQKSLARGHLLTYGVAYTRDRFDQIRDRRDRDLVTGRDDVYRGPL
                     VFPSKYFPESDVEELGVYLQDEMDLLGGRLKLVPGLRYDRYSLDADETDPVYLAGNAG
                     IETPVDLSSSALSPRLGLVMALPAGLSAFGQYARGFRAPAFSSVNSGFTNVASGYTTL
                     PNPDLEPETSATVEAGLRLTRSRGNASLTLFHNRFHDFIESVATGINPATQLLEFQPR
                     NVGEAVISGVELGGEARLGQAWTLRASAALIDGENETTEQPLNSIAPATGVLGLRYVR
                     PGRRWGGELITTLTAAKDEDDVDRTVVNQLATDGSEVVDLTLFFDPTDRISLTVGVFN
                     LFDVTWFAWPDVIGRTEGSPVLDRYTRPGRSFAAAFRLRR"
BASE COUNT          350 a          831 c          941 g          333 t
ORIGIN      
        1 gaggaggtcg ccgagccagg tgtcggaggt gaccacgagg aaggagcccg gcgcgccgaa
       61 cagcaccagc accggcaccg gccgcggcag ggcgagacgc tggagcgcct tccgggcctc
      121 ctggacgcgt gccgccatct cccggtccgc gcccgcccgc cgcgctgcgg tctggatccc
      181 ggcgaaggtg ccctccaccg aatcgccgcg cacggacagc acctcgacgc ccaccgcccg
      241 gagccgcggc tcgagcgcct tgtggatccg ctcgtcgacg accacgacct cggccccgga
      301 ggcggcgagc cgctcgaagt tgggggcgtg ggggctgccc aggtcggaca ccccctgggc
      361 gagcgcgccc ccctcggtcc ggcgcacccc ggcgacgacc cgggcacggc cgggcgccgc
      421 cgcccgcagc gcctcctcta tataaggaag gagcgtcgcg accttcagcg gctcggccgc
      481 ggcgaggggg accgccgcgg cgatcaggac gagcaggagg caggggagga gccggcgcat
      541 cagcccacct cctcgacggg caggcccgcg gcgagctctt ggtggagctc cgcgaacagg
      601 ccctcgcgcc cgggggcgag ctcctccccc ttttcccgat ggaggtagac gaggaggaac
      661 ggcgcggcgt cctcctcggt ccgcagctcg aagaacagca ccctccgccc cgagcgctgg
      721 tggatctcgt cgcgggagcg gaggtgccgg aagccgtcga gcttcaggtg ccagtgatag
      781 gaggcggtga tggcgttgac gtagccctgg gcgatgccaa aggcgccgac ctcgcagatc
      841 gcctcgaagg tgctcggccc cgactgcgag atcacccgca gcggcccgag cgccgccagg
      901 gcgtcgaaga agcggcgggc ggtccagctc gtgcccacgt tgccgtggct ggtcatggtg
      961 ctcatggtgt cgtcctcctc tcagcggcgg agccggaacg ccgcggcgaa gctgcgcccg
     1021 ggccgggtgt agcggtcgag gaccggggag ccctccgtcc ggccgatgac gtccggccag
     1081 gcgaaccagg tcacgtcgaa caggttgaag acccccaccg tcagcgatat ccggtcggtg
     1141 ggatcgaaga agagggtgag gtcgaccacc tcggagccgt cggtggcgag ctgattgacg
     1201 acggtgcggt cgacgtcgtc ctcgtccttg gcggcggtga gcgtggtgat cagctcgcca
     1261 ccccagcggc ggccgggacg gacgtagcgc agcccgagga cgccggtcgc gggggcgatg
     1321 gagttgagcg gctgctccgt agtctcgttc tcgccgtcga tgagggcggc gctggcgcgc
     1381 agggtccagg cttggccgag ccgggcctcg ccgccgagct cgacaccgga gatcacggcc
     1441 tcgccgacgt tcctgggctg gaactccagg agctgggtgg cgggattgat gcccgtcgcc
     1501 acgctctcga tgaagtcgtg gaagcggttg tggaagagcg tcaggctcgc gttcccccgg
     1561 gagcgggtga gccgcagccc ggcttcgacg gtggcgctgg tctccggctc taggtccgga
     1621 ttgggcaagg tggtgtagcc gctggccacg ttggtgaagc cgctgttgac cgagctgaac
     1681 gccggcgcgc ggaagccgcg ggcgtactgg ccgaacgccg agaggccggc cgggagcgcc
     1741 atcaccagcc ccagacgggg ggagagggcg gaggaggaga ggtcgaccgg cgtctcgatg
     1801 ccggcgttgc ccgcaaggta gaccggatcg gtctcgtcgg cgtcgagcga gtagcggtcg
     1861 tagcgcaggc cggggacgag cttgaggcgg ccgccgagga ggtccatctc gtcttgcagg
     1921 tagacgccca gctcttcgac atcgctctcc gggaagtact tcgaggggaa gacgagcggc
     1981 ccccggtaga cgtcgtcgcg gccggtaacc aggtcacggt cgcggcggtc gcggatctgg
     2041 tcgaagcggt cccgcgtgta ggcgactccg taggtgagaa ggtggccgcg ggcaaggctc
     2101 ttctggagct ccacctcgcc gccgaggccg tcctgctcga aggtgaagag gccgcggcgc
     2161 gcgatcccgc ctgccgccga ggtcagctcg tcgacggtgc gctgctcggt gtcgtcctcc
     2221 tgcgcgtgga ggcgccactc ggcgcgatcg aacagcgggc tcgtggcctc caggctctgg
     2281 gacaggctta cccgcgtccg ccgccgctcg tccacggcgt ccgcgtccgt cgtggtggtg
     2341 ccgggggtgg tcaccccgcg ggcggagtag atctcggtct cggcctcggc gcgcaggacc
     2401 tccgcggaca gcgtccacat cgacgagccg ccggggatga acgccagctt ggcga
//
LOCUS       DASPJI010000393         6195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_21747, whole genome shotgun sequence.
ACCESSION   DASPJI010000393 DASPJI010000000
VERSION     DASPJI010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21747"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="VEL74_10465"
     CDS             complement(<1..1279)
                     /locus_tag="VEL74_10465"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYG62994.1"
                     /translation="MSKDKILLVEDEPVIRFGIRDFLENKGFEVDEAADLESGRVVFQ
                     RSRPDAVILDYLLPDGNALDLIEELRTLDPEVPVVVLTGHGSIDLAVRAIKEGAENFL
                     TKPVELPALHVVLERALEGQRLRRRQAARVTRRDRDELDPFLGTSEAVRAYAREAAVA
                     MRSEGPVLIQGETGSGKGVLARWLHERGPRRDEPFVDLNCAGLSREFLDSELFGYARG
                     AFTGAVGDKKGLFEVAHKGSLFLDEMGDLDPALQPKVLKVLEEKRFRRMGETSDRQVD
                     VFLIAATNHEVTRLVREGKFRSDLYFRISTIPITVPPLRTRREDIPLLAEHLLRRVSR
                     DLGHPGLRLGRDAVEALQGYSWPGNVRELRNVLERAVLRTEGEALRAGDLQFATGLAV
                     TQGSGHSGHDLTLEEMERRQIEKILEEERGRVIN"
     gene            complement(1281..2426)
                     /locus_tag="VEL74_10470"
     CDS             complement(1281..2426)
                     /locus_tag="VEL74_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG62995.1"
                     /translation="MPTDEMLREKVWLAPDGEGEQPLLRALERAAIEWRTTFDAIEHP
                     VLLLGLDGRVRRLNRAARDLLGRAYRDLVGRHVSELMGGQPWEAVAALSARLLRTLTP
                     EICEARDDRSARTWEVEGCVSGIAEEGEGRVIIQLRDITKTVRLQESLRRSETMAVLG
                     AVVAGVAHEVRNPLFGMSAVLDAFENRFVDRSEYRPYLQLLRTELRRMSDLMQALLDY
                     GKPARFDMALRGLAEPLEAALALCRPLAESRKVTLAAGEPPADALVLLDLERLTQAIK
                     NVVENAIQHSPPGGEVALALRVLRIEGEDWLRLTVRDHGPGFSAADLPKIFEPFFSKR
                     KGGTGLGLSIVSRAVEGHGGKVGVANHPDGGGVVDIDLPCTLRSEGG"
     gene            complement(2560..3996)
                     /locus_tag="VEL74_10475"
     CDS             complement(2560..3996)
                     /locus_tag="VEL74_10475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HYG62996.1"
                     /translation="MSDETLADQTPVIAVVRVAAADAAPSGAPVTEYLMEAERVLKGY
                     LPGSTFVVRVPGGEMPGGAFLKVWGAPRLAPGGRALLFLTPGDDGGYEVVHLMLGAFH
                     LVGEGEEALAVRDLSEATPVRTSKAQEVDRPRRLAGFAGWLADRAAGLERPRDYMAPL
                     DGKAAVSLPFTLNNMADGVPLRWFRFDEGSTVAWRVHSAGQPGMGFDASAARLRDALD
                     VWNGDAGSNIRFGYLGSTTSNRGLTGTDGVHAVLFEDPGDRVPGRYSCPGGGVVALGA
                     AYAQSAVRSFRGQRFHDIIEADVVTNDGSECFFRDNPAGAAEVFAHELGHTLGLGHSA
                     ESDALMWPKAHNDGRGARLGHDDRAAVSSLYGDGSYTPPPPPPPPPANEPPAAPARLT
                     ARGVSASAIELAWEDKAGGAAEFSIERRFGKTFREVQVAAAGATRALIEGLKPGSYVF
                     RVRARSAAGASPYSNTVTVKLKAGRARR"
     gene            complement(4346..4711)
                     /locus_tag="VEL74_10480"
     CDS             complement(4346..4711)
                     /locus_tag="VEL74_10480"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HYG62997.1"
                     /translation="MTMEEQRLYRRMAAGVRVLYRGHSLRKPEAQYLTGIAEDLGLGG
                     MFISTRHPLPKGTLLSLEFHTSGDDGRPVRARAIVRWRRLFGRPRGMGVQFVEFEGLG
                     HRQVENWIDGVLLPEPIAC"
     gene            4996..5295
                     /locus_tag="VEL74_10485"
     CDS             4996..5295
                     /locus_tag="VEL74_10485"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HYG62998.1"
                     /translation="MTWIRNLFSPKPTPKGQEKAALGRNDLCWCGSGKKYKKCHLLKD
                     EANQREEAYAARVAAQVRQRQGKGGVVPTSSKSAGSKSQRRRPLPPEASKGPGGR"
     gene            5478..>6195
                     /locus_tag="VEL74_10490"
     CDS             5478..>6195
                     /locus_tag="VEL74_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG62999.1"
                     /translation="MGHSGRSRSPFLSLILGFLVLPSALSAQNPPAQPTAAPPPGEIL
                     QRVREAYAKLSSLSEEGLLEVVTSEGGAERIHRRRFELDLAAEGDFYFILGEMEPSEV
                     RETLWRSDGKVVHHDSLTETHKSMPSLAAGLAAMLGKPERAALVVPSLLAGDRAVWPE
                     AGAVSLQGEEPCGGRTCWVLAFPPGGGILESRHWIDRETLLVRKSVLRVGPETAARTY
                     RVDHAVHSVDGAAVETGLALD"
BASE COUNT          942 a         2245 c         2113 g          895 t
ORIGIN      
        1 cgttgatcac gcggccgcgc tcctcctcga ggatcttctc gatctgccgg cgctccatct
       61 cctccagcgt caggtcgtgg ccggagtggc cggagccctg ggtgacggcc aggccggtgg
      121 cgaactggag gtcgcccgcg cggagagcct ccccctcggt ccgcagcacg gcccgctcga
      181 ggacgttgcg cagctcccgg acgttacccg gccaggagta gccctggagc gcctccacgg
      241 cgtccctgcc gaggcgcagg ccggggtggc ccaggtcgcg ggagacgcgg cgcagcaggt
      301 gctcggcgag gagggggatg tcctcccgcc gggtgcgcag cggcggtacg gtgatcggaa
      361 tggtgctgat ccggaaatag aggtcgctgc ggaacttccc ctcgcgcacc agccgggtca
      421 cctcgtggtt ggtggcggcg atgaggaaga cgtccacctg ccggtcggag gtctccccca
      481 tccgccggaa ccgcttctcc tcgagcacct tgaggacctt cggctggagc gccggatcga
      541 ggtcccccat ctcgtcgagg aacaggctcc ccttgtgcgc cacctcgaac agccccttct
      601 tgtcgccgac ggcgccggtg aaggcgccgc gggcgtagcc gaacagctcg ctgtcgagga
      661 actcccgtga caggccggcg cagttgaggt ccacgaacgg ctcgtcgcgg cgcggcccgc
      721 gctcgtgcag ccagcgcgcc aggaccccct ttccgctgcc cgtctccccc tggatcagca
      781 ccgggccctc cgagcgcatc gccaccgccg cctcgcgggc gtaggcgcgc accgcctcgc
      841 tggtgcccag gaacgggtcg agctcgtccc ggtcgcggcg ggtgacgcgg gcggcctgcc
      901 gccggcgcag gcgctgcccc tccagggccc gctccagcac gacgtgcagc gccggcagct
      961 ccaccggctt ggtgaggaag ttctcggccc cctccttgat cgcccgcacg gcgaggtcga
     1021 tcgagccgtg gccggtgagc acgacgaccg ggacctctgg gtcgagggtc ctcagctcct
     1081 cgatgaggtc gagggcgttg ccgtcgggca gcaggtagtc caggatcacg gcgtccggcc
     1141 gcgaccgctg gaacaccacc cgcccgctct ccagatcggc ggcctcgtcg acttcgaacc
     1201 ccttgttctc caggaaatcg cggatgccga accggatgac cggctcgtcc tctacgagaa
     1261 ggatcttgtc ttttgacacc tcaaccccct tccgaccgca gcgtgcaggg aagatcgata
     1321 tcgacgaccc cgccgccgtc cgggtgattg gcgactccga ccttcccgcc atgcccttcg
     1381 acggcacggg agacgatgga gagcccgagc ccggtcccac ccttgcgctt ggagaagaac
     1441 ggctcgaaga tttttggcag gtccgccgcg gagaagccgg gcccgtggtc gcggaccgtc
     1501 aggcgcagcc agtcctcgcc ctcgatccgc agcacccgga gggcgagggc gacctcgccg
     1561 ccgggcggcg agtgctggat ggcgttctcc acgacattct tgatggcctg ggtgagacgc
     1621 tccaggtcga gcagcaccag ggcgtccgcc ggcggctcgc cggccgccag ggtgaccttg
     1681 cggctctcgg ccagcggccg gcagagggcg agggccgcct ccaggggctc ggccaggccc
     1741 cgtagcgcca tgtcgaaccg cgccggcttt ccatagtcga gcagcgcctg catcaggtcg
     1801 ctcatccgcc gcagctcggt gcgcagcagc tgcagatagg gccggtactc ggaccggtcc
     1861 acgaagcggt tctcgaaggc atcgaggacc gccgacatgc cgaagagcgg attgcgcacc
     1921 tcgtgcgcca cgcccgccac cacggcgccc agcaccgcca tcgtctccga gcggcgcagc
     1981 gactcctgca ggcgaaccgt cttggtgatg tcgcggagct ggatgatgac ccgcccctcc
     2041 ccctcctccg cgatgcccga gacgcacccc tccacctccc aggtgcgggc gctccggtcg
     2101 tccctggcct cgcagatctc gggggtcagc gtgcgcagga gccgcgcgct cagcgccgcc
     2161 accgcctccc agggctgccc ccccatgagc tcgctcacgt gccgccccac gaggtcgcgg
     2221 taggcgcgcc cgaggaggtc ccgggcggcg cggttgagcc gccgcacccg gccgtccagc
     2281 cccagcagca ggacaggatg ctcgatggcg tcgaacgtgg tacgccactc gatggccgcc
     2341 cgctccagcg cgcgcagcag gggctgctcg ccctcgccgt ctggcgccag ccatactttc
     2401 tcgcggagca tctcgtcggt gggcatggat tactaaagta tctgtgcaat aggtatacca
     2461 cctcctgaca cgcccgggcg ccaaaaaaag aaggatggaa gccgtcccga tcctggacgg
     2521 gcgtccagaa tcgggacggc ggtgccgcca gagccgccgt cagcggcggg cgcggccggc
     2581 cttgagcttg accgtgaccg tgttgctgta gggcgacgcg ccggcggcgc tcctggctct
     2641 cacccggaag acgtaggagc cgggcttgag cccctcgatc aaggcccggg tggcgccggc
     2701 ggcggcgacc tggacctcgc ggaaggtctt gccgaagcgg cgctcgatac tgaattcggc
     2761 cgcaccgccg gctttgtcct cccaggccag ttcgatggcc gaggcggaca ccccgcgcgc
     2821 cgtcaggcgg gccggcgcgg ccggcggctc attggccggc gggggcggcg gcggcggggg
     2881 cggcgtatag gagccgtcgc cgtagagcga cgagaccgcg gcccggtcgt catggccgag
     2941 acgggcgccg cggccgtcgt tgtgcgcctt gggccacatc agggcgtccg attccgcgga
     3001 gtggccgagg cccagggtgt ggccgagctc gtgcgcgaag acctcggcgg cgcccgccgg
     3061 gttgtcccgg aagaagcact cgctgccgtc attggtgacc acgtcggcct cgatgatgtc
     3121 gtggaaacgc tgcccacgga aggagcgcac cgcggattgc gcataggcgg cgccgagcgc
     3181 cacgacgccg ccgcccgggc agctgtagcg gccggggacg cggtcccccg gatcctcgaa
     3241 caggacggcg tgcacgccgt cggtgccggt cagcccgcgg ttggacgtcg tagaaccgag
     3301 atagccgaag cggatgttgc tgcccgcgtc gccgttccag acgtcgaggg cgtcccggag
     3361 gcgcgcggcc gaggcgtcga accccatccc cggctgcccc gcggagtgga cgcgccacgc
     3421 caccgtgctc ccctcgtcga aacggaacca ccgcaacggc acgccgtccg ccatgttgtt
     3481 caaggtgaag ggcagactga cggccgcctt cccgtccagc ggcgccatgt aatcccgcgg
     3541 ccgctcgagg ccggcggcgc ggtcggcgag ccagccggcg aagccggcga ggcggcgcgg
     3601 gcggtccacc tcctgcgctt tggaggtgcg gaccggagtc gcctcggaaa gatcgcggac
     3661 cgccagggcc tcctccccct cccccaccag atggaaggcg cccagcatga ggtggacgac
     3721 ctcatagccg ccgtcgtctc cgggcgtcag gaacagtagg gcccgcccgc cgggcgcgag
     3781 ccgcggcgcg ccccagacct tgaggaaggc gccgccgggc atctcgccgc cgggcacgcg
     3841 cacgacgaag gtgctgccgg gcagataccc cttgagcacc cgctccgcct ccatcagata
     3901 ctccgtcacc ggcgcgcccg agggcgccgc gtccgccgcg gccacgcgca ccacggcgat
     3961 caccggcgtc tggtcggcca gggtctcgtc actcatcatc acgtagctcg tcgccagcgc
     4021 tccacggctc ccggcggcaa gcagagcaag ggctaacaga aaacctttca tcctcatctc
     4081 ggatctcctc tcaggcttgt gattcagcgg aatcgcaacc ctcgtgccag atcggctgtc
     4141 cagagacaag gcccgtcaga tcaatggttt ggacgaatcg tccagacttt ggaacatttt
     4201 tatcgccaac ccctgttcga tcactggaca tccggtccag acgctggacg gcaggaaaca
     4261 tggggcgagc cgaaaaaaag agcgcggccc accaggagcc gcgcgggaag gtatccgagg
     4321 gaagggcccg ggaagattcg ggctgtcagc aggcgatggg ctcggggagc agcacgccgt
     4381 cgatccagtt ctccacctgc cggtgcccga gcccctcgaa ctcgacgaac tggaccccca
     4441 tgccgcgcgg gcggccgaag agccggcgcc aacgcacgat ggcccgggcc cgtaccgggc
     4501 ggccgtcgtc gccggaggtg tggaactcca acgagagcag agtgcccttg gggagggggt
     4561 ggcgcgtcga gatgaacatg ccgcccaggc ccaggtcctc ggcgattcca gtgagatatt
     4621 gcgcctcggg tttgcgcagc gagtgccccc ggtagagcac ccggaccccg gccgccatgc
     4681 gccggtacag cctctgttcc tccatcgtca tggcctctcc tctcctgctg cccgcgcagc
     4741 gatgaaatcg tatttcagcc gtccatagag caaaggatgg gccagcctcc cgggccctgg
     4801 ctttccccgg gagacccggg gctggaccct ccctttctgg tcccgaagcg ggacgggagg
     4861 tccgggatct ggacgatcgg gagccgaatg cgagatgatc ccccggctcc gctcccgatc
     4921 cgcgaggaat gaagggcccc ggagcgcccg ttgtatcgac cgtccgaaca ccgaaggagc
     4981 tcactcgtgt ccctgatgac ctggatccgc aatctcttct cccccaagcc gacccccaag
     5041 gggcaggaga aggccgctct tggccgcaat gacctgtgct ggtgcggaag cggcaagaag
     5101 tacaagaagt gccatctgct caaggacgag gcgaaccagc gcgaggaggc ctatgccgct
     5161 cgcgtcgccg cgcaagtgcg ccagcgccag ggcaaggggg gcgtcgttcc caccagctcc
     5221 aagtccgccg gctccaagtc gcagcgccgg cgccccctgc cgcccgaagc gagcaagggt
     5281 ccgggcggac gctgaggcaa cgccggtccc gccggtgcgc ctccaccctc agccgaaaac
     5341 actcgctgga tttttcggct ggggggtatc cgccctttgc gccgagcatt tcgggaactt
     5401 cttcgaagct cgaacagtcc aagaagatca gtgctgagcc aactcgccgt gggcgggtcc
     5461 cccagcaagg aggtcacttg ggtcattccg gtcgctccag gtcaccgttt ctgtccctga
     5521 ttctcggctt cctcgtcctc ccctctgcgc tttccgccca gaatccaccc gcccagccca
     5581 ccgcggctcc acctcctggc gagatcctcc agcgggtccg ggaggcctac gccaagctct
     5641 cctccttgag cgaggagggg ctcctcgagg tggtcacctc cgagggcggc gccgagcgga
     5701 tccaccggcg ccgcttcgag ctcgacctcg ccgcggaggg cgacttctac ttcatccttg
     5761 gcgagatgga gccgagcgag gtccgcgaga cgctctggcg cagcgacggc aaggtcgttc
     5821 accacgacag cctcaccgag acccataaga gcatgccctc gctggccgcc gggctggcgg
     5881 ccatgctcgg caagcccgag cgggcggccc tggtggtccc ctctctcctc gccggcgacc
     5941 gcgcggtctg gccggaggcc ggagccgtct ccctccaggg tgaggagccg tgcggcggcc
     6001 gtacctgctg ggtgctcgcc ttcccgccgg ggggcggcat cctcgagtcg cgccactgga
     6061 tcgaccgcga gaccctcctg gtgcggaaga gcgtcctgcg cgtcggtccc gaaacggccg
     6121 cgcggacgta tcgggtggac cacgcggtcc acagcgtgga cggcgccgcc gtcgaaaccg
     6181 gcctggccct cgacg
//
LOCUS       DASPJI010000394         5154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_657854, whole genome shotgun sequence.
ACCESSION   DASPJI010000394 DASPJI010000000
VERSION     DASPJI010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657854"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3199)
                     /locus_tag="VEL74_10495"
     CDS             complement(<1..3199)
                     /locus_tag="VEL74_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63000.1"
                     /translation="MSEPVSLTGLSREEKLALLERLARQKERAAKTFPLSFAQQRLWF
                     LDQLDPGNHVNNIFRALVWRGPLDEAALRRALTEIARRHESLRATFPSTANEPRQRVA
                     PPAEVPLSVEDLRGLTEDERRGHAAELARLESRHAFDLARGPLFRPRLLRLDAEEHVL
                     LLNMHHIVSDGWSMGLLFRELTALYRASAAGLPSPLPEPPLQYPDFAAWQRERFAGGA
                     LEEGLAFWREHLAGADTVLELPLDHPRPALESFRGAIHLFEVPAAEARALAALARREG
                     ATLFMTLTAAFEVLLHRFTGQSDFLLGTPVAGRDREELEDLIGFFANTLLLRARLGGD
                     PTFQDLLARVRRDTLDAYAHQDVPFERLVEELRPERHLSHNPLFQAMFALQNQPAGSV
                     EIPGVTLEPLRVERGLSKLDLTLEMGETASGLAGYFEYNTDLFDAGTIERLTGCFQAL
                     LAAMAANPERRISDLPLLAPDERRRLLAWNPPQSEAPATPVHRLIAARAAEAPEADAL
                     LFGGGRLSRLSYGELDRRANRLARHLRRLGVGPDVPVGICLERSLEMGEAILGVLKAG
                     GCYVPLDPAYPRERLAYMIEDTGMEVLLTQDGLAGTLSEVSHHLRVLRVDADAARWAG
                     ESDADPAVEVHPESVAYIIYTSGSTGRPKGVQVPHRAIANHATAIAARYGLTPADRVL
                     QFASLSFDIAAEELFPTWISGAALVPRPNGLFPSFLELERLLENHRVSVANIPTPYWH
                     EWVDDLARTGALPPSWLRLLVVGTEQAAPERLAAWCRLAGDRIRWINAYGPTESTITA
                     TVYEPSCHHSMWGEGEAGEERPLPPRVPIGQPIANLQAHVLDAAFEPVPLGVVGELYL
                     GGAGIARGYLHHPDRAAAVFLPDPFSGRPGARLYRTGDRARRLPSGELDFLGRADDQV
                     KIRGFRIEPGEIEAVLRRRPEVRECTVVVREDGGEKRLVAYAVPRAFARSAQGLLVDE
                     LRAVLSEALPSYMVPAAFVLLESLPMTPSGKIDRRALPAPEQPAGEVAASSTERSPLE
                     ELVAGIWSEVLRLDRFGDHQSFFELGGHSLLATQVI"
     gene            complement(3306..>5154)
                     /locus_tag="VEL74_10500"
     CDS             complement(3306..>5154)
                     /locus_tag="VEL74_10500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HYG63001.1"
                     /translation="FKGRRVTFAQLEDRANRLAHHLRRLGAGPGQLVGLCAERSLDLV
                     PAILGILKSGAGYAPLDPSYPAERLGWMLADTGAKILVGHERLLSALPEGPDGAVRVA
                     LDGDAASIIGEESAKNPVPLAGPADVAYVMYTSGSTGRPKGVAVSHRNVVRLVRETDF
                     ARFGPEEVFLQLAPVSFDASTLELWGPLLNGGRLAVFPPRQPSLEELGEVIARHRVTT
                     LWLTAGLFHQMVEGNLEGLRPVRQLLAGGDVLSPAHVRRVLAELPGTTLINGYGPTEG
                     TTFTCCHAMTEGSPVEHTVPIGRPIANTRVHVVDGSCRVLPVGLPGELLVGGDGLAVG
                     YLHRPELTAERFVPDPFDAPGGRLYRTGDQARWLADGTVEFFGRRDGQVKIRGYRIEI
                     GEIEAALAEHPEVREAAVVAREDAAGGRRLVAYVAAGTGDSADLVTALRSYLESRLPA
                     YMVPTAWVVLDSLPLTENGKVDRRALPAPEAPRRAERVAPRTPTEIAVAEIWREVLKV
                     ESVGVDDDFFALGGHSLAATRVLSRLRQRLGAQLSLANVFECSTLESLAAAVDEVAGA
                     GPAAMELIPAVPAPVATPAGEAARDLSDDQLDALLAEMITDGGVVELPK"
BASE COUNT          791 a         1805 c         1920 g          638 t
ORIGIN      
        1 agatgacctg cgtggccagg agcgagtggc cgcccagctc gaagaagctc tggtgatcgc
       61 cgaagcggtc caggcgcagc acctccgacc agatccccgc caccagctct tccagcggcg
      121 agcgttccgt cgaggacgcg gcaacctctc cggcgggctg ctccggcgcg ggcagcgccc
      181 tgcggtcgat tttgccgctg ggcgtcatcg ggagcgactc gaggagcacg aaggccgccg
      241 gcaccatgta ggacggcagg gcttcggaca ggaccgcccg cagctcgtcc accaggagcc
      301 cctgggcgga tcgggcgaag gcgcgcggga cggcgtaggc gaccaggcgc ttctcccccc
      361 cgtcctcccg caccaccacg gtgcactccc ggacctcggg ccggcggcgc agcaccgcct
      421 cgatctcccc cggttcgatg cggaagccgc ggatcttcac ctggtcgtcg gcccggccca
      481 ggaagtccag ctccccggaa gggaggcggc gcgcccggtc gccggtgcgg tagaggcggg
      541 cgccgggccg ccccgagaag gggtcgggca ggaagacggc cgcggcgcgg tccggatgat
      601 ggagatagcc ccgggcgatg ccggcgccac ccaggtagag ctcgcccacc acgcccagag
      661 ggaccggctc gaaagcggcg tccagcacgt gggcctggag gttggcgatc ggctggccga
      721 tggggactcg tggcggcagc ggtctctctt cgcccgcttc gccctctccc cacatcgagt
      781 ggtggcagga gggctcgtag accgtggccg tgattgtgga ctcggtgggg ccataggcgt
      841 tgatccaccg gatgcggtcg ccggccagcc ggcaccaggc cgccaagcgc tcgggcgccg
      901 cctgctcggt gcccaccacc aggaggcgga gccaggacgg agggagcgcg ccggtgcggg
      961 cgaggtcgtc cacccactcg tgccagtagg gggtggggat gttcgccacg gagacccgat
     1021 ggttctccag caggcgctcc aactccagga atgacgggaa caggccgttg ggccgcggca
     1081 ccagggcggc gcccgagatc caggtcggga acaactcctc ggcggcgatg tcgaaggaga
     1141 gggaggcgaa ctggaggacg cggtcggccg gggtcagccc gtagcgggcg gcgatggcag
     1201 tcgcgtggtt ggcgatcgcc cggtggggca cctgcacccc cttgggacgg ccggtcgagc
     1261 ccgaggtata gatgatgtag gccaccgact cggggtggac ctccaccgcc ggatcggcgt
     1321 cgctctcgcc agcccaccgc gcggcgtcgg cgtcgacgcg cagcacgcgg aggtggtgcg
     1381 acacctcgga gagggttccg gccaggccgt cctgggtcag caggacctcc atgccggtgt
     1441 cctcgatcat gtaggccagg cgctcccgcg ggtaggccgg gtccagcggg acgtagcagc
     1501 cgccggcctt gaggacgccg aggatcgcct cccccatctc cagagagcgt tccaagcaaa
     1561 tccccaccgg cacgtccggg cccactccga gacggcgcag gtggcgggcg aggcggttgg
     1621 cccggcggtc gagctcacca taggagagcc gggagagccg gccgccgccg aagaggaggg
     1681 cgtcggcctc gggggcctcc gccgcccgcg ccgcgatgag gcggtgcacc ggggtggccg
     1741 gcgcctcgct ctgcggcggg ttccaggcga ggagacgccg tcgctcgtcg ggcgccagca
     1801 gggggaggtc ggagatccgg cgctcggggt tggcggccat ggccgcgagg agagcctgga
     1861 agcagccggt caggcgctcg atggtgccgg cgtcgaagag gtcggtgttg tactcgaagt
     1921 agccggcgag cccgctggcg gtctccccca tctcgagggt caggtcgagc ttggacaggc
     1981 cgcgctccac ccgcagcggc tccagggtca cacccgggat ctccaccgag ccggcgggct
     2041 ggttctggag cgcgaacatg gcctggaaca gcgggttgtg ggagaggtgg cgctccggcc
     2101 gcagctcttc gaccaaccgc tcgaagggga cgtcctggtg agcgtaggcg tccagggtgt
     2161 cgcgccgcac ccgggccagc agatcctgga aggtcgggtc ccccccgagc cgggcgcgca
     2221 gcagcagggt gttggcgaag aaaccgatga ggtcctccag ctcctcgcgg tcacggccgg
     2281 ccacgggcgt gcccagcagg aagtcgctct ggccggtgaa gcgatggagc agcacctcga
     2341 aggcggcggt cagagtcatg aagagggtag ccccctcgcg ccgcgccagg gcggccaggg
     2401 cgcgggcctc ggccgccggc acctcgaaga ggtggatggc gccgcggaac gactccaagg
     2461 ccggacgcgg gtgatccagc ggcagctcca ggacggtgtc ggctccggcc aggtgctcgc
     2521 gccagaaggc gagcccctcc tccagtgccc cgccggcgaa gcgctcgcgc tgccaagcgg
     2581 cgaagtcggg gtattggagc ggcggctcgg gtagcggcga cggcaggccg gcggcggacg
     2641 cccggtagag cgccgtcagc tcacggaaga gcaggcccat ggaccagccg tccgagacga
     2701 tgtggtgcat gttgaggagg aggacgtgct cctcggcgtc caggcgcagg aggcggggcc
     2761 ggaacagcgg gccacgggcc aggtcgaagg cgtgccggga ctccaggcgg gccagctccg
     2821 ccgcgtggcc gcggcgctcg tcttcggtca ggccgcgcag atcctccacc gacagcggca
     2881 cctcggccgg aggcgccacc cgctgccgcg gctcgttggc cgtcgacggg aaggtggcgc
     2941 gcaacgactc gtgacggcga gcgatctcgg tcaacgcccg gcgcagcgcc gcctcgtcca
     3001 gcggcccccg ccagaccagg gcgcggaaga tgttgttgac gtgattgccc ggatcgagct
     3061 gatccaggaa ccagaggcgc tgctgcgcga acgacagggg gaacgtcttg gcggcgcgct
     3121 ccttttgccg cgccagacgt tcgagcaaag cgagcttctc ctcgcgcgac agacccgtca
     3181 gactcaccgg ctcactcatc gacttccttc ctccacagca gacatgaaca cgatcgaaat
     3241 caaaggatgc aggggagggg cttgccccct cccggaaaga accctccccg acaccgaagg
     3301 gcctcctact tcggcagctc caccaccccg ccgtcggtta tcatctcggc gagcagggcg
     3361 tcgagctgat cgtcggagag atcccgggcc gcctcgccgg caggcgtcgc caccggtgcc
     3421 gggacggccg ggatcagctc catcgccgcc ggtccggcac ccgccacctc gtccaccgcc
     3481 gccgccagag actccagggt cgagcactcg aagacgttgg ccagggagag ctgggcgccg
     3541 agccgctggc gcagccgtga cagcacccgg gtggccgcga gcgagtggcc gcccagggcg
     3601 aagaaatcgt cgtccacgcc gacgctctcc accttgagca cttcgcgcca gatctcggcc
     3661 accgcaatct cggtgggcgt gcggggcgcg acccgctcgg cgcgccgggg cgcctcgggc
     3721 gccggcaggg cccggcggtc caccttgccg ttctcggtca gcggcaggga atcgaggacc
     3781 acccacgccg tgggcaccat gtaggcgggc agcctggact ccagatagga gcgcagagcg
     3841 gttaccaggt ccgccgaatc acctgtgccg gccgcgacgt aggcgaccag ccggcggccc
     3901 ccggcggcgt cctcgcgggc gaccacggcc gcctcgcgca cctccggatg ctcggccaga
     3961 gccgcctcga tctccccaat ctcgatacgg tagccacgga tcttcacctg cccgtcacgg
     4021 cggccgaaga actccacggt gccgtcggcc agccagcggg cttggtcacc ggtacggtag
     4081 agccggccgc ccggcgcgtc gaacggatcg ggcacgaagc gctcggcggt gagctcggga
     4141 cggtggagat agcccacggc gaggccgtcg ccccccacca gcagctcgcc cggcaagccc
     4201 acgggcagga cacggcagga gccgtccacc acgtggaccc gggtgttggc gatcggccgg
     4261 ccgatgggaa cggtgtgctc caccgggctg ccctccgtca tggcatggca gcaggtgaag
     4321 gtggtcccct cggtgggacc gtagccgttg atcagcgtcg tccccggcag ctcggccagc
     4381 acccgccgga cgtgggcggg cgacaggacg tcgccgccgg ccaggagctg gcgcaccggc
     4441 cggaggccct ccaggttccc ctcgaccatc tgatggaaga ggccggcggt gagccagagg
     4501 gtggtcaccc gatgacgagc gatgacctcg cccagctcct cgagcgacgg ctggcgcggc
     4561 gggaagaccg ccaggcggcc gccgttgagc agcgggcccc agagctccag ggtggaggcg
     4621 tcgaaggata ccggagcgag ctggaggaag acctcctccg gtccgaagcg ggcaaaatcg
     4681 gtctcccgca cgaggcgcac cacgtttcgg tggctcaccg cgacaccctt gggacgcccg
     4741 gtggaacctg acgtgtacat gacgtaggcg acgtcggccg gaccggccag cggaactggg
     4801 ttcttggcgc tctcctcccc gatgatcgag gcggcgtcgc cgtcgagggc cacccgcacg
     4861 gcgccgtccg gcccctcggg gagagccgac agcagccgct cgtgcccgac caggatcttc
     4921 gccccggtgt ccgccagcat ccagcccaga cgctccgccg ggtacgaagg gtccagaggc
     4981 gcgtagccgg cgccgctctt caggatgccg aggatcgccg gcaccaggtc cagcgagcgc
     5041 tcggcacaga ggccgacgag ctggccgggg cccgccccca ggcgccgcag gtggtgcgcc
     5101 aggcggttgg ctcggtcctc gagctgggcg aaggtgaccc gtcggccctt gaag
//
LOCUS       DASPJI010000395        21214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_768475, whole genome shotgun sequence.
ACCESSION   DASPJI010000395 DASPJI010000000
VERSION     DASPJI010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 21214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21214)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21214
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768475"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VEL74_10505"
     CDS             complement(<1..238)
                     /locus_tag="VEL74_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63002.1"
                     /translation="MSNIEIRTGTIEMASAQFTFSKKVQSAQVALQGFDCRYGNKHEV
                     REIKVGAKVTNIAGAQVTVEAWASMKDEDHHFANG"
     gene            351..524
                     /locus_tag="VEL74_10510"
     CDS             351..524
                     /locus_tag="VEL74_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63003.1"
                     /translation="MESSLAALPFSQSGDPAYLERGLEVAGRARELAPEDPRPVGSLF
                     DLTLAAPEAPGRR"
     gene            1312..2133
                     /locus_tag="VEL74_10515"
     CDS             1312..2133
                     /locus_tag="VEL74_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYG63004.1"
                     /translation="MAEPTVAEPTAAQRTLVHYEVKEGVAYITLDDPPANTYTHEMMR
                     QLDDAVLQARFAKDVYVIVLAGAGSKFFCAGANINMLKSSDPYFKYYFCLHANETLNR
                     LEQTPKLVIAALEGHTVGGGLEIAMAADFLIAKKNSGKLGLPEINLGVLPGTGGTQRL
                     ARRISKSKAIQLMVTGELISFEDARDLGIVNDIFEGDDFMDQIHKYARQFCPPNRAAK
                     AVGRIKRSVQSGAEVPFESGLAIERELQQQLFESPDAKEGLDAYVSKRTPSFQGY"
     gene            2204..3415
                     /locus_tag="VEL74_10520"
     CDS             2204..3415
                     /locus_tag="VEL74_10520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HYG63005.1"
                     /translation="MPDQHDNDIVLAAPVRTPIGKFLGSLMPLSAADLGTAAAKAALE
                     RARLDPGQVDQVIFGHARQAGGGPNTARQVSWRAGVPVDRPAYTVNQACGSGLQAVLS
                     AARTILTGEARVILAGGTESMSNTPYLLTRARTGFRLGHGEIVDGMYKDGFNDPLSDL
                     VMGETAEELAKDMGIDRAEADEYALESQLRCERARAAGRFDPEVVPVKVPGRKGEVVM
                     EKDEHPRDGTTLESLRKLAPVFREGGTVTAGNASGITDGASAIVVASRRAALELGLPV
                     IARLTAWQVCGVEPRIMGIGPVPAIRTLLDRTGLDLGAIDEIELNEAFATQVIACMRD
                     LKLEHAKVNPDGGAISLGHPIGATGCRVLVTLLHGMQARDLQRGIATLCVSGGMGLAA
                     LVEREAAREAA"
     gene            3412..4149
                     /locus_tag="VEL74_10525"
     CDS             3412..4149
                     /locus_tag="VEL74_10525"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HYG63006.1"
                     /translation="MILVATGAEEYAVNYVAALKTVGVLAEEIRVVTPALLAEAGIDS
                     PGRELVPGAAGLVLCGGVDVDPARYGEEVLPHGRVEIDAARDAMEWDLLEAARAARLP
                     VWGVCRGIQTLNVFLGGSLWQDLPSQRPAGVEHSVVDPLDSLVHDVRVLDRGTSLGEI
                     LSRETPLVNSRHHQAPKRLAPGFTPVAASPDGLIEAAVLTNEEGQRWWVRGVQWHPEN
                     LVAMAQHRALWEDFVRVTTPATAPEGA"
     gene            4146..4916
                     /locus_tag="VEL74_10530"
     CDS             4146..4916
                     /locus_tag="VEL74_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYG63007.1"
                     /translation="MSTESVLYERRDRMATVTLNRPPLNILDIPTIHELNNVLGRIYQ
                     EEGLQLVVVRGAGERAFSAGVAVQDHTPDKIQPMLEGLHGAIRRLRDLAPVTVAAVRG
                     HCLGGGMEVALACDLVVATDDARFGQPEIELGCYPPVAAALYPSLIGYGRTLDLLLTG
                     RTLACEEAERLGLVTRRVEAGGLDAAVENLTAEITGKSAAVTRLTKKTVRAGRELGFD
                     AALAETERVYLEELARTEDMGEGIAAFMEKRRPVWKHR"
     gene            4939..5715
                     /locus_tag="VEL74_10535"
     CDS             4939..5715
                     /locus_tag="VEL74_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYG63008.1"
                     /translation="MPETILVEREGRVAILTINRPDKLNALNDQVREDMLEILGQIEN
                     DDAVGVVVITGAGDKSFIAGADIGEFAGRSPFDQRHAMRSPRIFDVMGSFSKPVIAMI
                     NGFCLGGGCELALSCDLRIASEKARFGQPEINLGLIPGGGGTQRLPRLVGLGHALRLI
                     LTGDMVGAAEAKEIGLVEMVVPHEELRAKTLELANKMASKSPLTVKVAKEAVRASQRL
                     AVEEGILYERDLFCLCFSTEDKEEGVKAFLEKRPAQWKGR"
     gene            5720..6874
                     /locus_tag="VEL74_10540"
     CDS             5720..6874
                     /locus_tag="VEL74_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYG63009.1"
                     /translation="MSAPHADLLALRSEFPILERSTYLINNSLGAMPRGVRDGLDTFA
                     REWEERGVRAWHEGWWEMPVNVGDLLAPLLGVRPGGISMHQNVAVAAEIFFSCFDYPA
                     ERNRIVYSELNFPNVMYLAEGERRKGAEIVTVPSDDGIGVPIERLLAAIDERTRLVPV
                     SHTLFRSAFVQDAQAIARRCREVGAILLLDVYQSTGVMPLELEAWGVDAAVGGSVKWL
                     CGGPGAGYLWVSPELAGRLYPTFAGWQADEEPFAFRPGAIRHAKNPWRFLTGTPNIPA
                     LYSCQAGYRIIASVGARKIRERSLALTDHLIALAEEAGFEVRTPRDHAHRGGTVSVWH
                     PDAERLSKELIAREVVCDYRPKAGIRLSPHFYNTEEECEHAIRTLRELAG"
     gene            7202..8260
                     /locus_tag="VEL74_10545"
     CDS             7202..8260
                     /locus_tag="VEL74_10545"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HYG63010.1"
                     /translation="MKLPTKVFLLTAVVAAGASLIALFLVAHLVTALGDADFARQVGW
                     NLLVIAVSAGALASAAAWLVVRRVTLPLKRLAATMSGMALSGALESDFPSAGGGGEVQ
                     LIEETFRSLVVSLKESQRARERSYVEAVGAVVTAADARDHETTGHSFRVALYAVALAK
                     RLGIHGEQLKAIEWGGLLHDVGKMVVPDGILRKGSPLTEDEWYVMKQHPVWGADMLGE
                     VSFLQPAALEIIQSHHERWDGTGYPRRLNGESIPLAARIFAVVDTYDAITSDRPYRRA
                     RSHQAAIAELQRVAGQQLDPRLVQAFVEMPEVEIRRLRDRCKRVHPGLSIPADLLDSL
                     TDGDDEKPAEKARPAASA"
     gene            8270..8689
                     /locus_tag="VEL74_10550"
     CDS             8270..8689
                     /locus_tag="VEL74_10550"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HYG63011.1"
                     /translation="MRTLSTGRCGPLHPTFSAEERRHLVREGIELFNRGDFYDAHETW
                     EDVWRSTTPEPKDLFQGLIQVAAGMHQILDLHRTAGPRTTLAKARRRLEPYLPAALGL
                     DVAGLLEAVRPWQEWLERGGERPAVPALRVIDWEAAV"
     gene            complement(8693..9619)
                     /locus_tag="VEL74_10555"
     CDS             complement(8693..9619)
                     /locus_tag="VEL74_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYG63012.1"
                     /translation="MSASASAAPFSLPRLIRDFPSLGEALKGAERAAATDAPILILGE
                     PGTGRSTVARALHAASPRAGGPLVEVDPGAIPSTLFESELFGYQAGAFTGAQRASEGR
                     VAQAQAGTLVLDHVEELPLAAQPKLLRLLAERRYAPLGAAEVEADVRFLAAGAEDLPR
                     RVERGAFRPDLFYRLEVVTFRLPPLRERRRDLPAILDHLLADLGDRLGRPLELAAPAR
                     AWIEEHPWPGNLRQLRNVLERGAILADGRLVDPPPPEGLLDGRPRPLLEMEVELIRHA
                     LAYTRGHQGKAAALLGISRKALWEKRKRHGIP"
     gene            complement(9627..10013)
                     /gene="rsfS"
                     /locus_tag="VEL74_10560"
     CDS             complement(9627..10013)
                     /gene="rsfS"
                     /locus_tag="VEL74_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HYG63013.1"
                     /translation="MSAETSTATPVSADTLARVRGAVAAAEDRKAVDLQVLHLQEVSD
                     FTDFFLICSGTSERQVQAIADAIDETLRKERVRPLHVEGYNRGQWVLLDYGDFVAHIF
                     QDETRRFYALERLWGDAPDVTQQFRQ"
     gene            complement(10010..10612)
                     /gene="nadD"
                     /locus_tag="VEL74_10565"
     CDS             complement(10010..10612)
                     /gene="nadD"
                     /locus_tag="VEL74_10565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HYG63014.1"
                     /translation="MKIGLFGGSFDPIHAGHLLPVREARRALGLERVIYLPTAVPPHK
                     PGRKLAPPYARYAMVELALLDEEGLFASPYELTLDRPAYTIDTLEHFQREMPEAEIHL
                     LIGGDSFVDLPLWRRWRDIAAAARLIVFTRPGWELEEAALPPELVSLARSERVHFLRQ
                     PPVDVSSTRLRALLARGEPVPDGAVSPRVVQYVQKYLLYR"
     gene            complement(10618..11631)
                     /gene="obgE"
                     /locus_tag="VEL74_10570"
     CDS             complement(10618..11631)
                     /gene="obgE"
                     /locus_tag="VEL74_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HYG63015.1"
                     /translation="MIFIDQTTIHVRGGRGGNGCMAFRREKYVPRGGPSGGDGGHGGS
                     VWLVGEEARNTLYHLQFTSLYAAERGRHGEGSNKTGRSGGDLDVAVPLGTQVYDAETG
                     ELLGELLEEGQRLLVAAGGGGGRGNARFATSTNQAPRRCEPGREGDERRLRLELKLLA
                     DVGVVGLPNAGKSTLISVVSAARPKIADYPFTTLVPQLGVVAEGPFSEPFVIADLPGL
                     IAGAAQGAGLGHQFLRHVERCRVLVHLVDLSNPEGSATEDLVTVERELEAFNPDLMRR
                     PRLMVGSKLDSALPERRDELRQAAGERGLPYFEISAATNEGVRPLVAEMARRLEAAKV
                     PVS"
     gene            complement(11724..11996)
                     /gene="rpmA"
                     /locus_tag="VEL74_10575"
     CDS             complement(11724..11996)
                     /gene="rpmA"
                     /locus_tag="VEL74_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HYG63016.1"
                     /translation="MAHKKGQGSSRNGRDSNPQHLGVKAYGGQQVTGGTIIVRQRGTV
                     FFPGLNVGRGKDDTLFAKRDGVVRFQNRGQKGKFVHIMAPEAAASE"
     gene            complement(12018..12338)
                     /gene="rplU"
                     /locus_tag="VEL74_10580"
     CDS             complement(12018..12338)
                     /gene="rplU"
                     /locus_tag="VEL74_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HYG63017.1"
                     /translation="MFAVIESGGKQYRVAPGDVIDVELLQGAGEQGSQVRFDRVLMVT
                     GDGEPRFGNPVVQGAAVTASLVAAVRGPKIRVFKMKRRKQYRRTRGHRQDLHRVRIDG
                     IELP"
     gene            12443..13282
                     /locus_tag="VEL74_10585"
     CDS             12443..13282
                     /locus_tag="VEL74_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYG63018.1"
                     /translation="MLRLFFLLAKALLFLAGARRRFIRSGPVRLAYYRLGPEKGEPWV
                     LLHGLGANAVSWYPVLRALKGECRLVVPELSMLGGTRGPRHGLGVAEASRVLADLIRR
                     ELGSRPVNLAGISLGGWIAVRLTLDRPDLVSRLLLIDAAGYRDQDWDEIERLIRVEDL
                     AGVDRLYGAMFVRPPWLLRRTRPAFLQTFTSRAVRDILEDLSEDDAYDDADLARIQVP
                     TGLIWGECDGLFTVATARAMTAALPHASLEVLPGCAHALHIESPRQLTAAIRRFRRAP
                     TRR"
     gene            13319..13477
                     /locus_tag="VEL74_10590"
     CDS             13319..13477
                     /locus_tag="VEL74_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63019.1"
                     /translation="MASPEYLVCLECETPCYTFEWEDGKLSEVLCTACGNDKPDQFAL
                     PEEIEDMA"
     gene            13792..14241
                     /locus_tag="VEL74_10595"
     CDS             13792..14241
                     /locus_tag="VEL74_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HYG63020.1"
                     /translation="MLSTTRWNPQTAYLTRDPFFRLVDSFFGNGAMTSGNEDVSNRSW
                     MPPVDIQETTDSYRLYAELPGLTKENIEITLENNVLRLSGERKFEKDAQKDNYHRIER
                     TYGAFSRSFTLPSQVNAEGVQAQFENGVLTLTVPKAEQAKPRRISIS"
     gene            complement(14751..16130)
                     /locus_tag="VEL74_10600"
     CDS             complement(14751..16130)
                     /locus_tag="VEL74_10600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG63021.1"
                     /translation="MSRRLLIGSVIFGLFILFDIALFGWLIFRSLSQREVDRVLGEMR
                     VEAESLAQQIHSRAEGQGKDLYTAVAVERQTRTYIDSILRERATVQTLTIRDRNGVLV
                     FEQHSRRREPVIEAEPLLGSQELPQAVEEMGEERVVIVEGPEIQVPIGEIGMIEIGIS
                     RTALDQRIQVLRRDLIEQASVIGVLTLLLLGTAYATIWWLMRRARVLELQAAEAERMA
                     YIGTLASGLAHEIRNPLNSLNLNMQMLEEEVDDLGSPPMSKRLLSITRSEISRLERLA
                     TDFLAYAKPRPLELEEVPAVWLLERARDVLGPDIQRREAQVELEDRSGGARVRVDPGQ
                     FTQLLLNLLQNALAATEESGQPPLLRLTAYRQGAAVVLELADNGIGIPPEEQGKIFDL
                     FYSTRKGGTGLGLAIVDRITRAHGGRVAVESTPGVGTTVKVEVPAVGGAAEAATRPAL
                     ETSYTPTAP"
     gene            16242..17921
                     /locus_tag="VEL74_10605"
     CDS             16242..17921
                     /locus_tag="VEL74_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYG63022.1"
                     /translation="MLTTTFLRSAGRLAPLLLATALLTAGAAGCDRREEPARPDGQAA
                     QAAETPRRGGTIVTGWISEPNGVNEFIVPSNQPTNELLHQIFLQLVDERPDFEQHPPT
                     FAPELARSYEFSPDHKTLTFHLRDDVLWSDGVPVTAEDVRFTWQAQTHPDVAWDNAFM
                     KESITDAEVVDPHTVRFHFSHAYARQMLDVNEGMIIPKHAWEKLPFAQWRQSREWFKE
                     NLVVSGPFTVASWQPQQQVVLQRNERYFKKDRPLLDRVVMRVIPEQSTLMAELNSGQV
                     DFVAQVGPGDAPSIRANPRLQLYAYWFSRLTVGVAWNLTREPFSDPEVRRALTLAIDR
                     QSIADTLWGELGRVASSPISTGFWGHDRSLRPWPYDPEQAKRILAARGWKDSDGDGIL
                     DRNGKRFSFEILSNAGNQQRIDAGVMIQEQLKRIGIQVVPRVVEFNTMLEQADAGRFD
                     AVTSGMTVDTGLDLTSYFHSRSVPPEGINFMRYSNPEVDRLIEQSLKVMEPQAAKPAL
                     DRIQQILHQDQPVTFLWESQRLSAASQRLRDAEPNVLRTLYNVEDWWVREP"
     gene            17969..18931
                     /locus_tag="VEL74_10610"
     CDS             17969..18931
                     /locus_tag="VEL74_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG63023.1"
                     /translation="MLGFLLRRLAASLILLVLVLTATFILMHLAPGDPTNREASRLTA
                     EQRENLRRIYGLDRPLPEQYLRWMTSVALRGDFGISWSQQRPVADALKAALPPTLLLA
                     FAALFVEYAIALPLGVAVARRPGSALDHGVRVVSLIIFSQPVFWLALMAILLFSQVWP
                     VLPSSLMRSLGYEQMSPGERLLDLLRHLALPALVLGVWASGATLRLVRGSLLEVMSRD
                     YIRTARAKGLSERRVVWVHGMRTALAPILQIFAVSLVGLLNGSLVAEVIFSWPGMGRL
                     AFEAVLARDYPVVLATTALAAVFVLAANFLADVIHALADPRVRE"
     gene            18928..19962
                     /locus_tag="VEL74_10615"
     CDS             18928..19962
                     /locus_tag="VEL74_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG63024.1"
                     /translation="MSRLRGGGPLFHWGLVLVAALLLVAVLAPLLSPYEPTEQTDPAV
                     SQYRPPGTVLAAVHLAHGDWRLADRVERTPEGLRLHRLGRVEELAAAEVRNLTPDGVA
                     DSRRFLLGSDKFGRDLLSRMIHGTRVSLGVALGSVLLALTLGVAVGSAAALGGPVIDG
                     LLMRMVDALLAFPSLFLLIVLAAFFRPSTLVIVIVLATGSWMGISRMIRAEILSLRSR
                     EFVLAARAMGQHPFAILVRHLLPNAFTPALIQATLLVGQLILVESTLSFLGLGIQPPT
                     PSWGNMIAEGREALARAWWIATFPGVAIAVTVIAFNFLGDGLRDALDPRQRRGSGRDE
                     RAAEAEAPAA"
     gene            20107..>21214
                     /locus_tag="VEL74_10620"
     CDS             20107..>21214
                     /locus_tag="VEL74_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63025.1"
                     /translation="MKRTLRAVLTGMAALLVAGAGWASPVSRIWTVNLGSEPLEVSIE
                     RTRGGSSRMDSLLVAPEEMAEVVRADDEEVRIVDGPDLLVINAPAGFDPTAIGLGGAT
                     LITPEIERPAGRLPAIRNKALAGSRLKRGEVASSTAVWTGSEPLTVRVGLHGSPALAE
                     VRATRLDGQILGYVTLAASREIEVTVDLRGFLDKHGYWGPVTREVLSRRGEVSASLGT
                     AGKRQAMQKAVGAASFNRRTHYTVFSATYPLYYYVDAGPANTCGELSSYRNGSWLFAS
                     GWLCTNGVGDSTKGPWYTANGNQTDNPLFIRWPDNSTTNNEWHIWDNNCPTATISTRT
                     STTFAGGATDVQWGVCFAAGAKITTYYRDLTTDRL"
BASE COUNT         3095 a         7686 c         7267 g         3166 t
ORIGIN      
        1 ggccgttggc gaagtgatgg tcctcgtcct tcatggacgc ccaggcctcg accgtgacct
       61 gtgcaccggc gatgttggtc accttcgccc cgaccttgat ctcgcggacc tcgtgcttgt
      121 tgccgtaccg gcagtcgaag ccctgcagcg ccacctgggc cgattggacc ttcttcgaga
      181 aggtgaactg ggcgctggcc atctcgatgg ttccggtccg gatttcgatg ttggacatgg
      241 cactctccct tgttcgttct gtccggtgtt ggacgcctcc gggagagagc aggcgccgta
      301 ccagctccgc accatagatg taagcacctg aaaggaaaga gcttgatgtc gtggagagca
      361 gcctggccgc ccttcccttc tcccagtcgg gcgatccggc ctatctggag cggggcctgg
      421 aggtggctgg acgggcgcgg gagctggcgc ccgaggaccc gcggccggtg ggcagcctct
      481 tcgacctgac gctggcggca ccagaggctc ctgggcgacg atgatggccg ggtcgtgcac
      541 gtggacgtgc ccgggggtac ccagccggag gccgtagccc aggagcgcga gcccctgctt
      601 ctggcggttc tccacatcca tccagtactg cgtggcgctc acccgcctct cggcgtggac
      661 cacgacgtga acggcatcct ggagcttcag gaagacgccg ccgtccggca cgaaagtctc
      721 cggcttcacc ccctcggtgt cgagatggag ggtcaccagg ttccagacca cgctccatct
      781 tcccaggggc agccagatgg gcgccgcgat gacggcgtgc ggcagggtca ggggatcgac
      841 gtagatccag atgtgcaggg tgcttcccag cagctgagcg ctctggtagg gcgtttcgga
      901 catggtcctc tgcctcccgt caggagccgg gcctcggggc acggccgggg ttggaaggga
      961 gctgccccga ctctatccca cccctcttcc ttcgttttgt cccgcatctg ctaaacggag
     1021 caccctgaag acatagacgg gattgcataa gtcccttcgc ctgccggccg ctggcggagg
     1081 gtggcgagca gacccgtcga tcctgcctcg cgggtggagc ccgaagtttt cgggcccctc
     1141 cctccctccc acccggcccg acgtctcctc tcctccctgg ggacggcggg ccgggtgaat
     1201 cctcccccgg tggaccgccc ttccatctcg tgcgagaatc ccccggagtc ctgccagggg
     1261 ggcacgcctt ccctgacccc aatcccatcc aacgtacggg agatcactac gatggctgag
     1321 ccgaccgttg cagaaccgac tgccgcccag cgcacccttg tccattacga ggtcaaggag
     1381 ggcgtcgcct atatcaccct cgatgacccg ccggccaata cctataccca cgagatgatg
     1441 cgccagctcg acgacgccgt cctccaggcc cgcttcgcca aggacgtcta tgtcatcgtc
     1501 ctcgccggcg ccgggtccaa gttcttctgt gccggcgcca acatcaacat gctcaagtcg
     1561 tcggacccct acttcaagta ctacttctgc ctgcacgcca acgagaccct caaccgcctc
     1621 gagcagaccc ccaagctggt catcgcggcg ctcgaggggc acaccgtcgg cggcggcctc
     1681 gagatcgcca tggcggccga cttcctcatc gccaagaaga actccggcaa gctcggcctg
     1741 cccgagatca acctcggcgt gctccccggc accggcggca cccagcgcct cgcccgccgg
     1801 atcagcaaga gcaaggcgat ccagctcatg gtcaccggcg agctcatcag cttcgaggac
     1861 gcccgggacc tcggcatcgt caacgacatc ttcgagggcg acgacttcat ggaccagatc
     1921 cacaagtacg cccgccagtt ctgcccgccg aaccgcgccg ccaaggccgt cggccggatc
     1981 aagcggtcgg tccagtccgg cgccgaggtg ccgttcgaga gcggcctcgc catcgagcgc
     2041 gagctgcagc agcagctctt cgagagcccc gacgccaagg agggcctgga cgcctacgtg
     2101 tccaagcgca cccccagctt ccaggggtat tgaccaacac cgcttccgag gcgcggcggg
     2161 cggagccccg gctcccgccc tgccgccctc ttcaggaatc gaaatgcccg atcagcacga
     2221 caacgacatc gtcctcgccg cgccggtgcg gacgcccatc ggcaagttcc tggggtcgct
     2281 catgcccctc tccgccgccg acctcggcac cgccgcggcc aaggcggccc tggagcgcgc
     2341 ccggctcgac ccgggccagg tcgaccaggt gatcttcggc cacgcccggc aggcgggcgg
     2401 cggccccaac accgcgcggc aggtctcctg gcgcgccggc gtgccggtcg accgccccgc
     2461 ctacacggtg aaccaggcct gcggctccgg cctccaggcg gtcctctcgg cggcgcgcac
     2521 catcctgacc ggcgaggcgc gggtgatcct cgccggcggc acggagagca tgtcgaacac
     2581 cccgtacctc ctgacccgcg cccgcaccgg cttccggctc ggccacggtg agatcgtcga
     2641 cggtatgtac aaggacgggt tcaacgaccc gctctccgac ctcgtcatgg gcgagaccgc
     2701 cgaggagctg gccaaggaca tggggatcga ccgcgccgag gccgacgagt acgcgctcga
     2761 gagccagctc cgctgcgagc gggcacgcgc cgccggccgc ttcgaccccg aggtggtccc
     2821 ggtcaaggtg cccggccgca agggcgaggt cgtcatggag aaggacgagc acccgcgcga
     2881 cggcacgacg ctggagtcgc tcaggaagct cgctccggtg ttccgcgagg ggggaacggt
     2941 caccgccggc aacgcctcgg ggatcaccga cggcgcctcg gcgatcgtcg tggcctcgcg
     3001 ccgcgccgcg ctggagctgg gcctccccgt gatcgcccgc ctcaccgcct ggcaggtctg
     3061 cggcgtcgag ccgcggatca tggggatcgg ccccgtgccg gccatccgca ccctcctcga
     3121 ccgcaccgga ctcgacctcg gcgccatcga cgagatcgag ctcaacgagg cgttcgccac
     3181 ccaggtcatc gcctgcatgc gggacctgaa gctcgagcac gccaaggtca acccggacgg
     3241 cggcgccatc tcgctcggcc acccgatcgg cgccaccggc tgccgggtgc tggtgaccct
     3301 gctgcacggc atgcaggcgc gggacctcca gcgcggcatc gccaccctct gcgtctccgg
     3361 cggcatgggt ctggccgcgc ttgtcgagag agaagcggcg agggaggcgg cgtgatcctg
     3421 gtcgccaccg gcgccgagga gtacgccgtc aactacgtcg ccgccttgaa aacggtgggc
     3481 gtgctggcgg aggagatccg tgtcgtgacc ccggccctcc tcgccgaggc agggatcgac
     3541 agccccggcc gcgagctggt ccccggcgcg gccggcctcg tcctctgcgg cggcgtggac
     3601 gtcgacccgg cgcgctacgg cgaggaggtg ctcccccacg gccgcgtgga gatcgacgcc
     3661 gcccgcgacg ccatggagtg ggacctgctg gaggcggcgc gcgcggcacg gctgccggtc
     3721 tggggggtct gccgcggcat ccagacgctc aacgtcttcc tcggcggctc tctctggcag
     3781 gatctgccgt cccagcgccc ggccggcgtc gagcacagcg tcgtcgatcc gctcgactcg
     3841 ctggtccacg acgtgcgggt gctggaccgc gggacctccc tcggcgagat cctctcccgc
     3901 gagacgccgc tggtgaacag ccgccaccac caggcgccca agcgcctggc ccccggcttc
     3961 acccccgtgg ccgcgtcgcc cgacgggctc atcgaggcgg cggtcctcac caacgaggag
     4021 gggcagcgct ggtgggtgcg gggggtccag tggcaccccg agaacctcgt cgccatggcc
     4081 cagcaccgcg ccctgtggga ggacttcgtg cgcgtgacga cgcctgcgac agccccggag
     4141 ggagcatgag caccgagagc gtcctctacg agcggcggga ccgcatggca accgtcacgc
     4201 tgaaccggcc gcccctgaac atcctggaca tccccaccat ccacgagctg aacaacgtgc
     4261 tcggccggat ctatcaggag gagggcctcc agctcgtcgt cgtgcgcggc gcgggcgagc
     4321 gcgccttctc cgccggggtg gcggtccagg accacacgcc ggacaagatc cagccgatgc
     4381 tcgaggggct ccacggcgcc atccgccggc tgcgcgacct ggcgccggtg acggtggcgg
     4441 cggtgcgcgg ccactgcctg ggcggcggca tggaggtcgc cctggcctgt gacctggtgg
     4501 tcgccacgga cgacgcccgc ttcggccagc cggagatcga gctcggctgc tatccgccgg
     4561 tggccgcggc gctctatccg tcgctgatcg gctacggccg caccctcgac ctgctgctca
     4621 ccggccgcac cctcgcctgc gaggaggccg agcgcctcgg cctggtcacc cggcgggtgg
     4681 aggcgggggg gctggacgcc gcggtggaga acctgaccgc cgagatcacc ggcaagagcg
     4741 ccgccgtcac ccggctgacc aagaagacgg tccgcgccgg acgggagctg ggcttcgacg
     4801 ccgccctcgc cgagaccgag cgcgtctacc tcgaagagct ggcccgcacc gaagacatgg
     4861 gagaagggat cgccgccttc atggaaaagc ggcgcccggt ctggaagcac cgttagaacc
     4921 ttctgaggag agatttcgat gcccgagacc attctggtgg agcgcgaagg ccgcgtcgcc
     4981 atcctgacca tcaaccgccc cgacaagctc aatgccctga acgaccaggt gcgcgaagac
     5041 atgctggaga tcctggggca gatcgagaac gacgacgcgg tcggcgtggt ggtgatcacc
     5101 ggcgccggcg acaagtcgtt catcgccggc gccgacatcg gcgagttcgc cggccgcagc
     5161 ccgttcgacc agcgccacgc catgcgcagc ccgcgcatct tcgacgtcat ggggagcttt
     5221 tccaagccgg tcatcgccat gatcaacggc ttctgtctgg gcggcggctg cgagctggcg
     5281 ctgtcatgcg acctgcgtat cgcgtcggag aaggcgcgct tcggccagcc ggagatcaac
     5341 ctcggcctga tcccgggcgg cggcggcacc cagcgcctgc cgcgcctggt cggcctcggc
     5401 cacgccctgc ggctgatcct gaccggcgac atggtcggcg ccgccgaggc gaaggagatc
     5461 ggtctcgtcg agatggtggt gccgcacgag gagctgcggg cgaagacgct ggagctggcg
     5521 aacaagatgg cgtccaagag cccgctcacc gtcaaggtgg ccaaggaggc ggtgcgcgcc
     5581 agccagcgcc tggcggtcga ggaggggatc ctctacgagc gcgacctgtt ctgtctctgc
     5641 ttctccaccg aggacaaaga ggagggcgtc aaggccttcc tggagaagcg ccccgcccag
     5701 tggaagggcc ggtgagccga tgagcgctcc gcacgccgat ctcctggccc tgcgctccga
     5761 gttcccgatc ctcgagcgct cgacctatct gatcaacaac tcgctcgggg ccatgccgcg
     5821 gggtgtccgc gacggcctcg acaccttcgc ccgtgagtgg gaggagcgcg gcgtgcgcgc
     5881 ctggcacgag gggtggtggg agatgccggt gaacgtcggc gacctcctcg cccctctgct
     5941 gggggtccgc cccggcggca tctcgatgca ccagaacgtc gccgtggcgg cggagatctt
     6001 cttctcctgc ttcgactacc cggcggagcg gaaccgcatc gtctactcgg agctgaactt
     6061 ccccaacgtc atgtacctgg ccgaggggga gcgccgcaag ggggccgaga tcgtcaccgt
     6121 cccttcggac gacggcatcg gcgtgcccat cgagcggctg ctggcggcga tcgacgagcg
     6181 cacccgcctg gtgccggtgt cgcacacctt gttccgcagc gccttcgtgc aggacgccca
     6241 ggcgatcgcc cggcgctgcc gcgaggtggg ggcgattctc ctcctcgacg tctaccagtc
     6301 caccggggtg atgccgctgg agctggaggc ctggggggtg gacgcggcgg tcggcggctc
     6361 ggtgaagtgg ctgtgcggcg gtccgggggc gggctacctc tgggtgagcc cggagctcgc
     6421 cggccggctc taccccacct tcgccggctg gcaggctgac gaggagccgt tcgcgttccg
     6481 gcccggcgcc atccgccatg ccaagaaccc ctggcgcttc ctcaccggca cgccgaacat
     6541 cccggccctc tactcctgcc aagccggcta ccgcatcatc gcctcggtcg gcgcccggaa
     6601 gatccgcgag cgctcgctgg cgctcaccga tcacctgatc gcgcttgccg aggaggccgg
     6661 cttcgaggtg cgcacgccgc gggaccatgc ccaccgcggc ggcaccgtct cggtctggca
     6721 ccccgacgcc gagcggctca gcaaggagct gatcgcccgc gaggtcgtct gcgactatcg
     6781 gcccaaagcc ggcatccgcc tctctcccca cttctacaac accgaagagg agtgcgagca
     6841 cgccatccgg acgctccgcg agctggccgg ctaggagcgc gtaccgcttc cacccggcag
     6901 ccgcacctct ataggtagga tgagccggca aaacctgccc ccgcttccag cgtcggggcg
     6961 accggagcga gggtggcatc gtttctccct gggaaaggat gctccccgct cgcggcgaca
     7021 gcccctccag ggctcacgca acgagtgagg aatggcgtat actgcatgcg cttccttcac
     7081 cgtgagagcc cgagtccggc caagtggcgc cttctgccgt agaggggcgc cgccggatgg
     7141 cgggtccgtc cggagacgtt cgggcaggtc ttttcgcagt cggcccaggg agtgatttga
     7201 gttgaagctg cccacgaaag tgttcctcct caccgccgtc gtggcggcgg gggcctctct
     7261 gatcgcactc ttcctggtcg ctcatctggt caccgctctc ggtgacgccg acttcgcccg
     7321 ccaggtcggc tggaacctgc tggtgatcgc cgtctccgcc ggtgccctcg ccagtgccgc
     7381 cgcctggctg gtggtccggc gcgtcaccct ccctttgaag cgcctggccg ccaccatgtc
     7441 gggcatggcc ctgtccggcg ccctggagag cgacttcccc agcgccggcg gcggcggcga
     7501 ggtccagctc atcgaagaga cgttccgttc gctggtggtg tcgctcaagg agtcgcagcg
     7561 ggcgcgcgag cgctcctacg tcgaggccgt gggcgcggtg gtgaccgccg ccgacgcccg
     7621 cgatcacgag accaccggcc actcgttccg cgtcgccctc tacgcggtgg ccttggcaaa
     7681 gcggctcggc attcatggcg agcagctcaa ggccatcgag tggggcggcc tgcttcacga
     7741 cgtcggcaag atggtggtgc cggacggcat cctgcgcaag gggagcccgc tgaccgagga
     7801 cgagtggtac gtgatgaagc agcacccggt ctggggggcg gacatgctgg gcgaggtgag
     7861 cttcctgcag ccggcggcgc tggaaatcat ccagagccac cacgagcggt gggacggcac
     7921 cggctacccg cgccggctga acggcgagtc gatccccctg gccgcccgca tcttcgcggt
     7981 ggtggacacc tacgacgcca tcacctcgga ccgcccctac cggcgggcgc gctcgcacca
     8041 ggccgccatc gccgagctgc agcgcgtcgc cggccagcag ctcgacccgc ggctggtcca
     8101 ggctttcgtc gagatgccgg aggtcgagat ccgccgcctg cgcgaccgct gcaagcgcgt
     8161 ccaccccggg ctcagcatcc cggccgacct cctcgactcg ctcaccgacg gcgacgacga
     8221 gaagcccgcg gagaaggcgc ggcccgccgc cagcgcgtag aatccgggcg tgcggacctt
     8281 gtcgaccggc aggtgcggcc ccctccaccc caccttctcc gccgaggagc gccggcacct
     8341 cgtgcgcgag gggatcgagc tcttcaaccg cggcgacttt tacgacgccc acgagacctg
     8401 ggaggacgtc tggcgctcca ccacgccgga gcccaaggac ctcttccagg gactgatcca
     8461 ggtcgccgcc ggcatgcacc agatcctcga cctgcaccgc accgccggcc cccgcaccac
     8521 cttggccaaa gcgcgccgcc gtctcgagcc ctacctcccc gcggccctgg ggctcgacgt
     8581 cgccgggctg ctggaagccg tgcggccgtg gcaggagtgg ctggagcgcg ggggggagcg
     8641 gccggcggtg ccggccctgc gggtgatcga ctgggaggcg gcggtctgac ccctacggga
     8701 tcccgtgccg cttcctcttc tcccacaacg ccttgcggct gattcccagg agggcggcgg
     8761 ccttcccctg gtgaccgcgg gtgtacgcca gggcgtggcg gatcagctcc acctccatct
     8821 ccagcagcgg acgggggcgg ccgtcgagga gcccctcggg gggcggcggg tccaccagcc
     8881 ggccgtcggc gaggatggcg ccgcgctcga gcacgttgcg gagctgccgg aggttgcccg
     8941 gccaggggtg ctcctcgatc caggctcggg ccggcgccgc cagctccagc ggccgtccga
     9001 ggcggtcccc caggtcggcc agcaggtgat cgaggatggc ggggaggtcc cggcggcgct
     9061 cgcgcagcgg cggcagccgg aaggtcacca cctccagacg gtagaagagg tcgggccgga
     9121 acgcgccgcg ctccacccgc cgggggaggt cctcggcgcc ggcggcgagg aagcgcacgt
     9181 cggcctccac ctcggcggcg cccagcggcg cgtagcggcg ctcggcgagc aggcgcagca
     9241 gcttgggctg cgcggccagc ggcagctcct cgacgtggtc gagcaccagg gtccccgcct
     9301 gggcctgcgc cacccgcccc tcgctcgccc gctgcgcgcc ggtgaaggcg cccgcctggt
     9361 agccgaagag ctcgctctcg aagagggtgc tggggatcgc gccggggtcc acctccacca
     9421 gcggcccgcc ggcgcggggg ctcgcggcgt ggagggcgcg ggcgacggtc gaccggccgg
     9481 tgccgggctc gccgaggatc aggatggggg cgtcggtggc ggcggcgcgc tcggcccctt
     9541 tcagggcttc acccaacgag gggaaatcgc ggatcagccg cggcaggctg aagggggccg
     9601 ccgatgccga cgcgctcacg ggcggctcac tgccggaact gctgggtcac gtcgggggca
     9661 tccccccaga ggcgctccag ggcatagaac ctgcgggtct cgtcctggaa gatgtgggcg
     9721 acgaagtcgc cgtagtcgag cagcacccac tgcccgcggt tgtagccctc gacgtgcagc
     9781 gggcgcacgc gctccttgcg caacgtctcg tcgatggcgt cggcgatggc ctggacctgg
     9841 cgctcgctgg tgccgctgca gatcaggaag aagtcggtga agtcgctgac ctcctgcaga
     9901 tgcagcacct ggaggtcgac ggctttgcgg tcttcggccg cggcgacggc gccgcggacg
     9961 cgggccaggg tgtcggcgga tacgggggtg gctgtagagg tctcggcgct catcggtaca
    10021 agaggtattt ctgcacgtat tgtaccaccc gcggcgaaac cgccccatcg gggaccggct
    10081 cgccccgcgc caggagggcc cgcaggcggg tggaggagac atcgaccggc ggctggcgca
    10141 ggaaatggac ccgctcggac cgcgccaggc tcaccagctc cggcggcagc gcggcctcct
    10201 ccagctccca gcccggacgg gtgaagacga tcaggcgcgc cgcggccgcg atgtcgcgcc
    10261 agcggcgcca gagcggcagg tcgacgaagg agtcgccgcc gatcagcagg tggatctccg
    10321 cctcgggcat ctcgcgctgg aagtgctcga gggtgtcgat ggtgtaggcc ggccggtcca
    10381 gggtcagctc atacggggag gcgaagagcc cctcctcgtc gaggagggcc agctccacca
    10441 tcgcgtaccg ggcgtacggg ggcgccagct tgcggcccgg cttgtggggg ggcaccgccg
    10501 tgggcaggta gatgacccgc tccaggccca gggcgcgccg agcctcccgc accggcagga
    10561 ggtggccggc gtggatcggg tcgaagctgc cgccgaagag gccgatcttc atggcgctca
    10621 ggacaccggg accttcgcag cctccaaacg ccgcgccatc tcggcgacga gcgggcgcac
    10681 cccctcgttg gtggcggcgc tgatctcgaa gtaaggaagg ccgcgctctc cggccgcctg
    10741 ccgcagctcg tcccgccgct cgggcagcgc cgagtcgagc ttgctcccca ccatcagccg
    10801 cggacggcgc atcaggtcgg gattgaacgc ctccagctcg cgctccaccg tcaccagatc
    10861 ctcggtggca ctcccctcgg ggttcgacag gtccaccaga tggacgagga cgcggcagcg
    10921 ctcgacgtgg cggaggaact ggtggccgag cccggccccc tgcgccgcgc cggcgatgag
    10981 ccccggcagg tcggcgatga caaagggctc ggagaacggc ccctcggcga cgacgccgag
    11041 ctgcggcacc agggtggtga acggatagtc ggcgatcttc ggccgcgccg ccgagacgac
    11101 gctgatcagc gtcgacttgc cggcgttggg caggccgacg acgccgacgt cggcgaggag
    11161 cttgagctcc agccgcagcc gccgttcatc cccttcgcgc cccggctcgc agcggcgcgg
    11221 cgcctggttg gtggaggtgg cgaagcgggc gttgccccgg ccgccgccgc cgccggccgc
    11281 caccagcagg cgctggccct cctccagcaa ctcgccgagg agctcgccgg tctcggcgtc
    11341 gtagacctgg gtgcccaggg ggacggcgac atccaggtcg ccgccgctcc ggccggtctt
    11401 gttggagccc tcgccgtgcc ggccgcgctc ggcggcgtac agcgaggtga actggaggtg
    11461 gtagagcgtg ttgcgcgcct cttcgcccac cagccacacc gagccgccgt gaccgccgtc
    11521 gccgcccgac gggccgccgc gcggcacgta cttctcgcgc cggaaggcca tgcagccgtt
    11581 gccgccacgg ccgccgcgga cgtggatcgt ggtctggtcg atgaagatca aggtcgtcgg
    11641 gctcgaaggt gagaagagag gacgggagcc ggacgggccg gctcccggat cactcgcggg
    11701 attgcctgga gatgaatcgg agattactcg gaagcggcgg cttcgggggc catgatatgg
    11761 acgaacttgc ccttctggcc cctgttctgg aagcgcacca ccccatcccg cttggcgaac
    11821 agcgtgtcgt ccttgccgcg gccgacgttc aggccgggga agaagacggt gccgcgctgc
    11881 cgcacgatga tggtgccgcc ggtgacctgc tgcccgccgt aggccttgac gcccaggtgc
    11941 tgcgggttgg agtcacggcc gttgcggctg gagccctgtc cctttttatg tgccattgtc
    12001 gttgactcct tcttgtccta cggcagctcg atgccgtcga tgcggacccg gtgcaggtcc
    12061 tgccggtgac cgcgggtccg ccggtactgc ttgcggcgct tcatcttgaa gacccggatc
    12121 ttcggcccgc gcacggcggc caccagcgag gcggtcaccg ccgcgccctg gaccaccgga
    12181 ttgccgaacc gcggctcgcc gtcgcccgtc accatcagga cccggtcgaa ccggacctgg
    12241 ctgccctgct caccagcacc ctgcaggagc tccacgtcga tgacgtcgcc gggcgcgacg
    12301 cggtattgtt tgccaccact ttcgatcact gcgaacatag acactcctga cagtcgtcgt
    12361 accagacgcg tggacgggag aacctaccac agaggtcccg cccggtcaac catccgggcc
    12421 ctcctgttat tctccggggg ccatgctgcg cctcttcttc ctgctcgcca aggccctgct
    12481 cttcctggcg ggggcacggc gccggttcat ccgctccggc ccggtgaggc tggcctacta
    12541 ccggctgggc ccggaaaagg gggagccctg ggtgctgctc cacgggctcg gcgccaacgc
    12601 ggtgagctgg tacccggtgc tgcgcgccct caagggggag tgccggctgg tggtgccgga
    12661 gctctccatg ctcggcggca cccgcggccc gcggcacggg ctgggcgtcg ccgaggcctc
    12721 gcgggtgctg gcggacctca tccgcaggga gctcggcagc cggccggtca acctggcggg
    12781 catcagcctc ggcggctgga tcgcggtccg cctgaccctc gaccgccccg acctcgtctc
    12841 ccggctcctc ctcatcgacg ccgccggcta ccgcgaccag gactgggacg agatcgagcg
    12901 gctgatccgg gtggaggacc tcgccggcgt cgaccggctc tacggcgcca tgttcgtccg
    12961 ccccccctgg ctgctgcggc ggacccgccc cgccttcctt cagaccttca cctcgcgcgc
    13021 ggtgcgcgac atcctggagg acctgtcgga ggacgacgcc tacgacgacg ccgacctcgc
    13081 ccgcatccag gtgcccaccg ggctgatctg gggggagtgc gacggcctgt tcaccgtcgc
    13141 caccgcccgg gcgatgaccg cggccctgcc ccacgcctcc ctggaggtgc tgcccggctg
    13201 tgcccacgcc ctccacatcg aaagcccgcg gcagctcacg gcggccatcc gccgcttccg
    13261 gcgcgccccc acccggcgct agactggtcc cgacgacctt atttataagg aggaagccat
    13321 ggccagcccc gagtacctgg tctgcctgga gtgcgagacc ccctgttata ccttcgagtg
    13381 ggaggacggg aaactgtcgg aggtcctctg tacggcctgc ggcaacgaca agccggacca
    13441 gttcgcgctg cctgaagaga tcgaagacat ggcgtagggt cccctcccct cctgccagcc
    13501 ctcccctcag ctcccctggt gtcagccgga tcggtccgat cggtcggatc ggccgatcgc
    13561 ctttgcctgt gcgcgccacc caaaatctga gcacgactac atgagatctt gagccccggc
    13621 tttgtccggc aagtagacct gatggatttc agctaagatt tctactgagg ggggttgaca
    13681 agatgtgagc gccgatctat catgcgcatc gtcgggcggg tggaccggag ccaatcccgg
    13741 acaccgagcc agaaccgaac cgatgacgcg atgaacaagg aggatcaaac aatgctcagc
    13801 acgactcgtt ggaatcccca gaccgcttat ctcacccgcg atccgttctt ccgcctggtc
    13861 gacagcttct tcggcaacgg cgccatgacc tccggcaacg aggacgtctc caaccggagc
    13921 tggatgccgc cggtggacat ccaggagacc accgactcct accggctgta tgccgagctg
    13981 cccgggctga ccaaggagaa catcgagatc accctcgaga acaacgtcct gcggctgtcc
    14041 ggtgagcgga agttcgagaa ggacgcccag aaggacaact accaccgcat cgagcggacc
    14101 tacggcgcct tctcccgctc cttcaccctg ccgagccagg tgaacgccga gggcgtgcag
    14161 gcgcagttcg agaacggcgt gctgaccctc acggtcccca aggccgagca ggccaagccg
    14221 cgccggatct ccatcagctg agccggccga ccgggccgga tcgaaggggg tggccccgcc
    14281 tcacggcggg gcctttcttt ttccagggaa atcttatgca ggggaggggc ttgccccatc
    14341 ggcgctaccc tcagcgccgc cagggggcgg gtggtgcctc cccccctggg cggtcgggtg
    14401 gcaggaagcg ggggaggtca aggagggggg ttttcacacc gctccgcgct ccgcgaccgg
    14461 ccccctaggc gctgactcaa cggttgtccg gaaggatttt cgtcgcagac cagggttccc
    14521 gagggtcgca acccgaggag aacatcgcag agccgtgttc attcgtcgca gacgacctcc
    14581 gctctgcgac gaatgaaggc agctctgcga cgattgagcg ctactctgcg gcgaacgaac
    14641 gatgctctgc gacgaatgga cgcaactctg cgatgtttca ggacagctct gcgacgtttc
    14701 aggacagctc tgcaacgatc ggatcggaag ggcaagcccc tctcctacat ctaaggtgca
    14761 gtcggggtgt acgacgtctc gagcgccggg cgggtggcgg cctcggcggc tccgccgacg
    14821 gccggcacct cgaccttgac ggtggtgccc acgccgggcg tgctctccac cgccacccgc
    14881 ccgccgtggg cgcgggtgat ccggtcgacg atggcgagcc ccaggccggt gccccccttc
    14941 cgcgtcgaat agaagaggtc gaagatcttc ccctgctcct cgggcgggat gccgatgccg
    15001 ttgtccgcca gctcgagcac caccgccgcc ccctgccggt aggcggtgag ccggagcagc
    15061 ggcggctggc cggactcctc ggtggcggcg agggcgttct gcagcaggtt gaggaggagc
    15121 tgggtgaact ggccggggtc gacccgcacc cgggcgccgc cgctcctgtc ctccagctcc
    15181 acctgcgcct cccgccgctg gatatccggg ccgagcacgt cgcgcgcccg ctccagcagc
    15241 cagaccgcgg gcacctcctc gagctccagg gggcgcggct tggcataggc gaggaagtcg
    15301 gtggccaggc gctccagccg gctgatctcc gagcgggtga tcgagaggag ccgcttgctc
    15361 atcggcggcg agccgaggtc gtcgacctcc tcctccagca tctgcatgtt gaggttcagc
    15421 gagttcagcg ggttgcggat ctcgtgcgcc aggcccgagg cgagggtgcc gatgtaggcc
    15481 atgcgctcgg cctcggccgc ctgcagctcc aggacccggg cgcgccgcat cagccaccag
    15541 atggtggcgt aggcggtgcc gagcagcagc agggtgagga cgccgatgac gctcgcctgc
    15601 tcgatcaggt cgcgccgcag cacctggatg cgctggtcca gcgcggtgcg gctgatgccg
    15661 atctcgatca tcccgatctc gccgatgggc acctggatct ccggcccctc gacgatcacg
    15721 accctctcct cccccatctc ctccaccgcc tgggggagct cctggctgcc gagcagcggc
    15781 tcggcctcga tgaccggctc gcgccgccgg ctgtgctgct cgaagaccag cacgccgttc
    15841 ctgtcgcgga tggtcagcgt ctggacggtg gcgcgctcgc gcaggatgga gtcgatgtag
    15901 gtccgcgtct ggcgctcgac ggcgacggcg gtgtagaggt ccttgccctg accctcggcg
    15961 cggctgtgga tctgctgggc cagcgactcc gcctcgaccc gcatctcgcc gagcacgcgg
    16021 tcgacctccc gctgcgacag cgagcggaag atcagccagc cgaagagggc gatgtcgaac
    16081 aggatgaaga ggccgaagat gaccgagccg atcagcagcc ggcgggacat gacggagtct
    16141 gcggcccgtt ccatacgcat gaagaggata acaccggcat cgccaccctc cttccggggg
    16201 gcttcatttg acgcccccgc cggagtagga tggccgcgcc gatgctaacg acgacattcc
    16261 tccgttccgc ggggcgcctc gcccctctgc tgctcgccac cgccctgctg accgccgggg
    16321 ccgccggctg tgaccgccgg gaagagcccg cccggcccga cggccaggcc gcacaggccg
    16381 ccgagacgcc gcggcgcggc ggcacgatcg tcaccggctg gatctccgag ccgaacggcg
    16441 tcaacgagtt catcgtcccg tcgaaccagc ccaccaacga gctgctgcac cagatcttcc
    16501 tgcagctcgt ggacgagcgg cccgacttcg agcagcaccc gccgacgttc gcccccgagc
    16561 tcgcccgctc gtacgagttc tctcccgacc acaagacgct caccttccac ctgcgcgacg
    16621 acgtgctctg gagcgacggc gtgccggtga ccgccgagga cgtgcgcttc acctggcagg
    16681 cccagaccca ccccgacgtg gcctgggaca acgccttcat gaaggagtcc atcaccgacg
    16741 ccgaggtggt ggacccgcac accgtccgct tccacttctc gcacgcctat gcccgccaga
    16801 tgctcgacgt caacgagggg atgatcatcc ccaagcacgc ctgggagaag ctccccttcg
    16861 cccagtggcg gcagagccgc gagtggttca aggagaacct ggtggtctcc ggcccgttca
    16921 ccgtcgcctc ctggcagccg cagcagcagg tggtcctcca gcgcaacgag cgctacttca
    16981 agaaggaccg gccgctgctc gaccgcgtgg tgatgcgggt gatcccggag cagagcaccc
    17041 tgatggccga gctgaacagc ggccaggtgg acttcgtcgc gcaggtcggg ccgggcgacg
    17101 cgccgtcgat ccgggccaac ccgcgcctcc agctctatgc ctactggttc tcccgcctga
    17161 ccgtgggggt cgcctggaac ctgacccgcg agccgttctc cgatccggag gtgcgccgcg
    17221 ccctcaccct cgccatcgac cggcagtcca tcgccgacac cctctggggc gagctcggca
    17281 gggtggcgag ctcgcccatc tcgaccggct tctggggcca cgaccggtcg ctgcggccct
    17341 ggccctacga ccccgagcag gcgaagcgca tcctggcggc ccgcgggtgg aaggacagcg
    17401 acggcgacgg catccttgac cgcaacggca agcggttctc cttcgagatc ctgagcaacg
    17461 ccggcaacca gcagcgcatc gacgccggag tgatgatcca ggagcagctc aagcggatcg
    17521 gcatccaggt cgtgccgcgc gtggtggagt tcaacaccat gctggagcag gccgacgccg
    17581 ggcggttcga cgccgtcacc tccggcatga cggtggacac cggcctcgac ctcacctcct
    17641 acttccacag ccgctccgtg ccgcccgagg gcatcaactt catgcgctac tccaacccgg
    17701 aggtggaccg gctcatcgag cagtcgctga aggtgatgga gccgcaggcg gccaagcccg
    17761 ccctcgaccg catccagcag atcctccacc aggaccagcc cgtcaccttc ctctgggagt
    17821 ctcagcgcct gagcgcggcg agccagcgtc tgagggacgc cgagccgaac gtcctcagga
    17881 ccctctacaa cgtcgaggac tggtgggtcc gcgagccctg agggcgcccg aggacgccct
    17941 gaccggcgcc tgaaggtccc aagacgccgt gctcggcttc ctcctgcggc gcctcgccgc
    18001 ctcgctgatc ctcctcgtcc tcgtcctcac cgccaccttc atcctgatgc acttggcgcc
    18061 gggcgacccg accaaccggg aagcctcgcg cctgaccgcg gagcagcggg agaacctgcg
    18121 gcggatctac gggctcgacc ggccgctccc cgagcagtac ctgcgctgga tgacgtccgt
    18181 ggcgctgcgc ggcgacttcg ggatctcctg gtcgcagcag cggccggtgg cggacgcgct
    18241 caaggcggcg ctgccgccga ccctcctcct cgccttcgcc gccctgttcg tggagtacgc
    18301 catcgccctg ccgctgggcg tcgccgtggc gcggcggccc ggctcggcgc tcgaccatgg
    18361 ggtgcgggtg gtgtcgctga tcatcttctc gcaacccgtg ttctggctcg ccctgatggc
    18421 gatcctgctc ttcagccagg tctggccggt gctgccgtcg agcctgatgc gctccctcgg
    18481 ctacgagcag atgagcccgg gggagcggct cctcgacctc ctccggcacc tcgccctgcc
    18541 ggcgctggtg ctcggagtct gggcgagcgg cgcgacgctg cggctggtgc gcggcagcct
    18601 gctcgaggtg atgagccggg actacatccg caccgcgcgg gcgaagggcc tctcggagcg
    18661 gcgcgtcgtc tgggtgcacg gcatgcggac ggcgctcgcg ccgatcctcc agatctttgc
    18721 cgtctcgctg gtcggcctgc tcaacggctc gctggtcgcc gaggtgatct tctcctggcc
    18781 cgggatgggg cggctcgcct tcgaggcggt cctcgcacgc gactatccgg tggtgctggc
    18841 gaccaccgcg ctcgccgccg tcttcgtcct ggcggccaac ttcctggccg acgtgatcca
    18901 cgcgctggcc gacccgaggg tacgggagtg agccggcttc gcgggggtgg ccccctcttc
    18961 cattggggcc tggtcctggt cgccgccctg ctgctggtgg cggtcctggc gccgctcctc
    19021 tcgccctacg agccgacgga gcagaccgac cccgcggtct ctcagtatcg gccgccgggc
    19081 acggtgctcg cggcggtcca cctcgcccac ggcgactggc ggctggctga cagagtggag
    19141 cggacgccgg aggggctgcg cctccatcgc ctgggacggg tcgaggagct cgccgccgcg
    19201 gaggtgcgca acctcacccc ggacggcgtg gccgacagcc gccgcttcct gttgggcagc
    19261 gacaagttcg ggcgcgacct cctgtcgcgg atgatccacg gcacccgcgt ctccctgggg
    19321 gtggcgctgg gctcggtgct gctggcgctg acgctgggcg tggcggtcgg ctcggccgcg
    19381 gcgctcgggg ggccggtgat cgacggcctg ctgatgcgga tggtcgacgc cctgctcgcc
    19441 ttcccgtcgc tcttcctgct gatcgtgctg gcggccttct tccggccgag cacgctggtg
    19501 atcgtcatcg tcctggcgac cgggagctgg atggggatca gccgcatgat ccgggcggag
    19561 atcctgagcc tgcggagccg cgagttcgtg ctggccgcgc gggccatggg ccagcacccg
    19621 ttcgccatcc tggtccgcca cctgctgccg aacgccttca ccccggcgct gatccaggcg
    19681 acgctgctgg tcggccagct catcctggtg gagtcgacgc tctccttcct gggcctgggc
    19741 atccagccgc cgacgccgag ctggggcaac atgatcgccg agggccgcga ggccctcgcc
    19801 cgcgcctggt ggatcgccac cttccccggc gtcgccatcg ccgtcaccgt catcgccttc
    19861 aacttcctgg gcgacggtct gcgggacgcg ctggacccgc ggcagcggcg cgggagcggc
    19921 cgggacgagc gggcggccga agccgaagcg ccggcggcct gaaacgtttc cccccctccc
    19981 gacactccgg acagtagtcc ccatcgctag atgcgcacat ccaccctggc ggctggccgt
    20041 ccggggcgct ggagatgtct tttcgctccg gggcattccg tcccggggct ttctggaggg
    20101 gtgtctatga agagaacgtt gcgcgcagtg ctcaccggaa tggccgcgct gctggtggcc
    20161 ggggcgggat gggcttcgcc ggtgtcacgg atctggaccg tgaacctggg gagcgagccg
    20221 ctggaggtgt cgatcgagcg gacccgcggc ggcagctcgc ggatggacag cctcctcgtc
    20281 gcgccggagg agatggccga ggtggtgcgg gcggacgacg aggaggtccg gatcgtcgac
    20341 gggcccgacc tcctggtcat caacgcgccc gccggtttcg acccgacggc gatcggcctc
    20401 ggcggcgcca ccctcatcac acccgagatc gagcgtcccg ccgggcgcct gcccgccatc
    20461 cgcaataagg cgctcgccgg cagccggctg aagcggggcg aagtcgcctc ctccaccgcc
    20521 gtctggaccg gaagcgagcc gctgaccgtg cgcgtcgggc tccacggctc cccggcgctc
    20581 gccgaggtcc gcgccacccg gctggacggg cagatcctgg gctacgtcac cctcgccgcc
    20641 tcccgcgaga tcgaggtgac ggtcgacctc cgcggcttcc tcgacaagca cggatactgg
    20701 ggcccggtca cccgcgaggt cctgtcccgg cgcggcgagg tctccgcgtc cctggggacc
    20761 gccggcaagc gccaggcgat gcagaaggcg gtgggcgccg cctcgttcaa ccggcgcacc
    20821 cactacaccg tgttcagcgc gacgtacccc ctgtactact acgtcgacgc cggaccggcg
    20881 aatacctgcg gcgagctctc cagctaccgc aacggctcct ggctcttcgc ctcgggctgg
    20941 ctgtgcacca acggtgtcgg cgattccacc aaggggccct ggtacaccgc caacggcaac
    21001 cagaccgaca atcccctctt catccgctgg cccgacaact cgacgacgaa caacgagtgg
    21061 cacatctggg acaacaactg cccgaccgcg accatcagca cgcggacgtc gaccaccttc
    21121 gcgggcggtg ccaccgacgt gcagtggggc gtctgcttcg cggccggcgc caagatcacg
    21181 acctactacc gggacctcac cacggaccgc ctct
//
LOCUS       DASPJI010000396         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_657865, whole genome shotgun sequence.
ACCESSION   DASPJI010000396 DASPJI010000000
VERSION     DASPJI010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657865"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VEL74_10625"
     CDS             <1..556
                     /locus_tag="VEL74_10625"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HYG63026.1"
                     /translation="TQQGAPLKIGGLPDPEARVTRWAIDIPYGLSILAYGDPQATVTG
                     LEDIPRENWPNVVLVHLSFQVMVACGVAMAALTLWIGFLWLRRRRLPDSPRLLRLILL
                     VSPLGVLAIKAGWFVTELGRQPWIIQGVMRTRDALTPMPGLVVPLLAITLVYVVLGVI
                     VVFLLKRHVISTVPTAGAQAKRAA"
     gene            557..1555
                     /locus_tag="VEL74_10630"
     CDS             557..1555
                     /locus_tag="VEL74_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HYG63027.1"
                     /translation="MDLALATALVIALALNVYAISGGADYGGGVWDLLARGPRAARQR
                     DLIAHSIGPIWEANHVWLILAIVALFTGFPAAYARVTTILHVPLLLMLLGIVARGAAF
                     SFRSYGRPEGEDQGHWGRVFAISSLVTPLLLGIILGAIASGEMEAVPRSVAAFFDPWV
                     GLFPFAVGVYALVMFAFLAAVYLCNEAEEADLAEDFRRRALASGVLLGVLAAAVLLAA
                     GLRVPDLLHELTRTPWSWALHAATALAALFTFRALWSRRYRLARAGAALQVSLILWGW
                     ALAQYPYLVKPDLTVGSAAAPDVTLRLLLGALAVGAVVLVPSFLYLYRVFGKLRRA"
     gene            complement(1452..1802)
                     /locus_tag="VEL74_10635"
     CDS             complement(1452..1802)
                     /locus_tag="VEL74_10635"
                     /inference="COORDINATES: protein motif:HMM:NF008333.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HYG63028.1"
                     /translation="MKLLQIDFPSQGPWGEEMAEAFAGLAGMIAGTPGLLWKIWTESS
                     VAHEAGGIYLFADEESLDRYLKEHTERLRSFGIDDIRALKLDAACRTPGRDRGRTAPG
                     RPLPRPGLPGGAAG"
BASE COUNT          243 a          707 c          730 g          364 t
ORIGIN      
        1 gactcagcag ggggcgccgc tcaagatcgg cggcctgccg gacccggagg cgcgggtcac
       61 ccgctgggcg atcgacatcc cctatggcct cagcatcctg gcctacggcg atccgcaggc
      121 gacggtgacc ggcctcgagg acatcccgcg ggagaactgg ccgaacgtgg tcctcgtcca
      181 cctgtcgttc caggtgatgg tggcctgcgg cgtggcgatg gcggcgctga cgctgtggat
      241 cggtttcctg tggctgcgcc ggcgccggct gccggactcg ccgcgcctgc tgcggctcat
      301 cctcctggtc tcgccactgg gggtgctggc gatcaaggcg ggctggttcg tcaccgagct
      361 cggccgccag ccctggatca ttcagggggt gatgcggacg cgggacgccc tgacgcccat
      421 gccggggctg gtggtgccgc tcctggcgat cactctggtg tacgtcgtcc tcggcgtgat
      481 cgtcgtcttc ctgttgaagc gccacgtgat ctcgacggtg cccaccgccg gcgcccaggc
      541 caagagggcg gcttagatgg acctcgccct ggccacggcg ctggtgatcg ccctggcgct
      601 caacgtctac gcgatctcgg gcggcgcgga ctacggcggc ggggtctggg acctcctggc
      661 ccgcggcccc cgggcggcgc ggcagcgcga cctgatcgcc cactcgatcg gcccgatctg
      721 ggaggcgaac cacgtctggc tgatcctggc gatcgtggcg ctcttcaccg gcttccccgc
      781 ggcctacgcg cgggtcacca cgatccttca cgtgccgctc ctcctgatgc tgctgggtat
      841 cgtcgcccgc ggcgccgcgt tcagcttccg gtcgtacggc cggcccgagg gggaggatca
      901 gggccattgg ggccgcgtgt tcgccatctc cagcctggtg acgccgctgc tcctgggcat
      961 catcctgggg gccatcgcct cgggggagat ggaggcggtt ccgcgctcgg tggcggcgtt
     1021 cttcgatccc tgggtgggac tcttcccctt cgccgtcggc gtctatgcgc tggtgatgtt
     1081 cgccttcctc gcggcggtct acctgtgcaa cgaggccgag gaggcggacc tggcggagga
     1141 tttcaggcgg cgggcgctgg cctcgggggt gctgctggga gtgctggcgg cggcggtcct
     1201 gctcgccgca gggctgcgcg tgccggacct cctccacgag ctgacgcgga cgccgtggtc
     1261 ctgggcgctg cacgccgcca ccgccctcgc ggcgctcttc accttccggg ccctctggag
     1321 ccggcgctac cgtctggccc gcgccggagc cgccctccag gtgtcgctca tcctctgggg
     1381 ctgggcgctc gcgcagtatc cctacctggt caagccggac ctcaccgtcg gctcagccgc
     1441 cgcgccggac gtcaccctgc ggctcctcct gggagccctg gccgtgggag cggtcgtcct
     1501 ggtgccgtcc ttcctctatc tctaccgggt gttcggcaag ctgcgtcgag cttgagggcg
     1561 cggatgtcgt cgattccgaa ggagcggagc cgctcggtgt gctccttcag ataacggtcc
     1621 agcgactcct cgtccgcgaa gagatagatg ccgccggctt cgtgggcgac ggagctctcg
     1681 gtccagatct tccagagcag gcccggcgtg ccggcgatca tcccggcgag acccgcgaag
     1741 gcctcggcca tctcttcgcc ccacgggcct tgtgatggga agtcgatctg tagcagcttc
     1801 atggtggtcc tcccttgatc tctgaagaaa gagacgacgg tggaccggcg tttccctcgc
     1861 cgtgtactct ggagatggcg ctccgagtcg agggcgagga ggtgagagat ggtccgaatc
     1921 cccaaagccc tggccaccct gctggtcgtg acgaccctga gctgcaccgc caacgcgggc
     1981 ggtggggacc ggagcggtgg acagccggcc ggaagctggc gcgaggtgat ccacgacgcc
     2041 gacg
//
LOCUS       DASPJI010000397        10018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_488445, whole genome shotgun sequence.
ACCESSION   DASPJI010000397 DASPJI010000000
VERSION     DASPJI010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10018)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10018
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488445"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VEL74_10640"
     CDS             <1..733
                     /locus_tag="VEL74_10640"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYG63029.1"
                     /translation="MEKHAVSKLIGSPPGYVGHEEGGQLTERVRRQPYSLILFDEIEK
                     AHPDVANLLLQILEDGVLTDAYGNQVDFRNTLVLMTSNIGSKLVLRGGRMGFGEGSEQ
                     ADFRRIEEEILAELRRSFSPEFINRIDEVIVFNPLGELELRSIVDILLDDVNLTLSER
                     GLTVTVSPDARQWLLGKADIEPSTGARPLRRVIQRHIQDAVSEILIHQHGGESIERID
                     VDLEGGELKFDSVHLPTETPAITAP"
     gene            824..3319
                     /gene="bamA"
                     /locus_tag="VEL74_10645"
     CDS             824..3319
                     /gene="bamA"
                     /locus_tag="VEL74_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HYG63030.1"
                     /translation="MKLDFVVNRRSSYSPLNPIGLRGRIWRSLLVLALLPLLVSRGVP
                     AVAQEGGIAGAPAAEAPRIDAVEFRGLKALTEETLLYYLGLEVGQPLDQALLDQNIKT
                     LWERSLIDDIQVEAIPAALPAGNAVRLVITVAERPVLRSIEYEGLKRISKTDIQDKIA
                     TQRIRVREGEPLSLGELQRVKALIEELHAEKGYRFASARYNVEDIGTNEKRVVFTVDE
                     GNRVRIEDIAFEGNTVYNDLRLRLSMRKTKETGLISRFSKKDIYDPAKLQEDLEKVRD
                     LYRAAGYKNVVIGEPQIDVRALKPEAAATSEQKRRMFITVPIEEGDRWRFGEISIDGN
                     DVYTDQALLRVFAHEPNGWLRSKVIDDGVKSVTDLYHNTGYVYAQVEPELVEKPGRVA
                     DLIVHVQEGEQFTVGRIEFQGNERTQDKVLRREIRLYESGVVNIAAIRNSVLKINQLG
                     YFKLNEEDPVEFVADNEDKTVDLIFKGQESERTELQFGGGWSEVDEFFGQFSINTKNF
                     LGRGEQVGVSLQQGKYRNFYDVSYFVPWFMDRRQSIGLRAFDQDLDYRGFDARERYAR
                     QSRGAILTYGRSFRLFQSASISYNRSRYNDQSTVLDELTGNPIALPTLSFDNSSLRPA
                     YLFDSRDNPYEPTRGKRLSLSAEYAGGFLGGDTYFLRPELAFSMFQPVGSLPVRQVVA
                     LNVEAGLVDPFDPKEYPLSPLDLFFLGGENSIRGFSFRGISVRNADGTVRRDTFGAIV
                     GGDKYAQVNLEYHFLLGGPFRVLVFADAGNVYADDQSFDLSNLRYTAGAELRVLVPVF
                     GAPLRFIYALNLDPLPGDDDRFENFQFSIGTSF"
     gene            3338..3889
                     /locus_tag="VEL74_10650"
     CDS             3338..3889
                     /locus_tag="VEL74_10650"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HYG63031.1"
                     /translation="MLIRRFPKTLLALGLGLALSAPAMAQGPKIAVIDTEQILLASNT
                     GKKALADLKKLQEQKETEGKNRSQEIKDLQTKLNDGRLSLAADKIGDMEKQLEDKVIA
                     LKRFQDDANRELGKRRDEILASVDQKVMPVINQLGKEQGYTLIFRKFESGLIYADEAV
                     DITGQVIQRLDGGGGAAQGSKGK"
     gene            3894..4976
                     /gene="lpxD"
                     /locus_tag="VEL74_10655"
     CDS             3894..4976
                     /gene="lpxD"
                     /locus_tag="VEL74_10655"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HYG63032.1"
                     /translation="MTRRLAELAELVGGTVEGDPERSIEGVRTLDAAGPRDLSFLNHP
                     RYREQASASQAGALLVPRTLAGLAGLPGDTGKDLLVVSDAAYALSRLIALFNEPEARE
                     PGVHPTAVLEPGCAVDPAAHVGPYVVIGAGSRVAAGAVLHAHVVVGRRCAVGERAVLY
                     PHVVLYDGTEVGAGGIVHSGAVLGSDGFGYATARGVHHKVPQVGRVVVHEDVEIGANT
                     TIDRATLGETVIGAGTKIDNLVQIGHNVRVGRHCILCGQAGIAGSTHLGDGVVMAGQA
                     GAAGHIHLGDRAQVAAKSAALATVPAGTTVAGIPAVEMRKWRRQAVLVSRLEEMSRRL
                     RALERRLHLEHGSGAPEAPEDIGEEE"
     gene            4978..5433
                     /gene="fabZ"
                     /locus_tag="VEL74_10660"
     CDS             4978..5433
                     /gene="fabZ"
                     /locus_tag="VEL74_10660"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HYG63033.1"
                     /translation="MSESEPTPKWDIEWIQSILPHRYPILLVDRVLEIEPKKRIVAIK
                     NVTINEPFFAGHFPGRPVVPGVLLVEGMAQAGGILLLQDIPDREKKLVYFMGIEEAKF
                     RRPVVPGDQVRYEVEVLRLRTAYCKLAGKIMVDGNLAAEAVITSAMVNR"
     gene            5444..6286
                     /gene="lpxA"
                     /locus_tag="VEL74_10665"
     CDS             5444..6286
                     /gene="lpxA"
                     /locus_tag="VEL74_10665"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HYG63034.1"
                     /translation="MAMTTELIHPQALVDPGAVLGEGVRIGPFAVVGPGVVLGDGTEV
                     GAGAQVQGPARIGRENRIFPYACIGFEPQDLKFRGEEVSVEIGDRNQFREFTTVHRGT
                     SKGGGVTRIGSDNLFMAYTHVAHDCQVGSRTIFANNATLAGHVEVHDDANISAFTSVH
                     QFCRVGRHAYIGGYSVITMDALPFVKTVGISPAFYGLNSIGLKRKGFSPEGVKRLDRA
                     VRILVRSGLNTSQALERMRAELAGDPDVDYLIAFVEGSSRGVHKSMPRGGRSGGAEAP
                     PEAS"
     gene            6283..7227
                     /locus_tag="VEL74_10670"
     CDS             6283..7227
                     /locus_tag="VEL74_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYG63035.1"
                     /translation="MSAPLQSLRVGVVGVGAMGRHHVRILSSLPGAELVGIHDPRIEA
                     AEAVAAEHGARVFTSLDELAGGIDAAVLAAPTVTHAETGCALLERGLHLLVEKPIASS
                     LAEADRLIAAADGEPGRRRVLAVGHVEFYNPAVQSLLGLGVPPRFVEIQRLGVFSPRS
                     LDVDVVLDLMIHDLQILHGLDPSPVHEVRATGIAVLSPRIDIANARVELASGCVANLT
                     ASRVSAERVRKLRAFLPSRYYSLDYQAQEIKGYRLEEGEGARRIAPDDLPVEKGEPLR
                     RELEAFVAACRGESAPLVTGGAGRRALETALAVVEAIG"
     gene            7318..8190
                     /gene="mtnP"
                     /locus_tag="VEL74_10675"
     CDS             7318..8190
                     /gene="mtnP"
                     /locus_tag="VEL74_10675"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HYG63036.1"
                     /translation="MSDIRIGVIGGSGLYQMEGLVVREERRLETPFGDPSDVYVIGEL
                     DGRPVAFLPRHGRGHRLLPTELNYRANIFGFKQLGVEQLISVSAVGSLKIEYKPTDIV
                     VPDQFFDRTRHRVDTFFGGGLVAHVSLAKPVCPRLIGHAAEAARAAGATVHQGGTYVC
                     MEGPQFSTRSESESYRREGFDIIGMTNLQEARLAREAEICYVSLSMVTDYDCWHETEE
                     AVSGEAVMEVVRQNAKMSQEVVRHMLSRLGDGRERGCLCESALAMSLITERAAIPAET
                     VQKLQPIIGRYIKA"
     gene            8191..8961
                     /gene="surE"
                     /locus_tag="VEL74_10680"
     CDS             8191..8961
                     /gene="surE"
                     /locus_tag="VEL74_10680"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HYG63037.1"
                     /translation="MTRILVTNDDGIFSQGIRLLASALSELGEVTVVAPDREQSAMGH
                     ALTLSRPLRMQKVEEKWYAVDGTPTDCVNLAVLSLLQESPPDLVVSGINFGLNLGDDV
                     TYSGTVSATFEGTLLGIPSVAFSQEVAEGFSFAPAARFARDLCEVLLAAGELPRDLLL
                     NVNVPAGPVQGVSFTRLGRRIYKQSVIEKLDPRGRKYYWIAGTPQWQKAEGTDYEAIS
                     QNRISVTPLHLDLTYYPGLESFGSLWDKLSALTFHKPE"
     gene            8958..9611
                     /locus_tag="VEL74_10685"
     CDS             8958..9611
                     /locus_tag="VEL74_10685"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HYG63038.1"
                     /translation="MSDFAVQRERMVRDHIAARGIKDERVLAALRRIPRHLFVRDHLR
                     SRAYGDHALPIGASQTISQPYVVAKMSEMLEVGKEHSVLEIGTGSGYQTAILAVLARR
                     VYSLERIAELAHQAIPRMRELGLDNVKVQVFDGTVGWSEWAPYDRILVTAAAPKVPEP
                     LLEQLAPGGTLLVPEGSINAQRLVLYKKSARGEVRRKEGEEVAFVPLLGRHGWKADK"
     gene            9626..9883
                     /locus_tag="VEL74_10690"
     CDS             9626..9883
                     /locus_tag="VEL74_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HYG63039.1"
                     /translation="MLLSGRVQGVGFRWFTRAAAQELGLAGLVRNLPDGRVEVEVAGG
                     PEGLEAFRRRLREGPPGARVEALEEQELDTVPAWDGFRIDR"
     gene            9907..>10018
                     /locus_tag="VEL74_10695"
     CDS             9907..>10018
                     /locus_tag="VEL74_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017289709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HYG63040.1"
                     /translation="MNDLKQYIREVPDFPKPGILFYDITTLLQNPLALRMT"
BASE COUNT         1593 a         3439 c         3481 g         1505 t
ORIGIN      
        1 catggagaaa cacgccgtgt ccaagctcat cggctcgccg ccgggctacg tcggccacga
       61 ggaggggggc cagctcaccg agcgcgtgcg gcggcagccc tactcgctga tcctgttcga
      121 cgagatcgag aaggcgcacc cggacgtcgc caacctgctg ctgcagatcc tggaggacgg
      181 cgtgctcacc gacgcctacg gcaatcaggt ggacttccgc aacacgctgg tgctcatgac
      241 ctccaacatc ggctccaagc tggtgctgcg cggtggccgc atgggcttcg gcgaggggtc
      301 cgagcaggcc gatttccggc gtatcgaaga agagatcctg gctgagctgc ggcgcagctt
      361 cagccccgaa ttcatcaacc ggatcgatga agtgatcgtg ttcaacccgc tcggcgaatt
      421 ggagctgcgg tcgattgtcg acatcctgct cgacgacgtg aacctcacgt tgagcgagcg
      481 ggggctgacg gtcacggtct cccccgacgc caggcagtgg ctgctgggga aggcggacat
      541 cgagccgtcc accggcgctc ggccgctccg ccgggtcatc cagcggcaca tccaggacgc
      601 ggtgtcggag atcctcattc accagcacgg cggcgagtcg atcgagagga tcgatgtgga
      661 cctcgagggc ggagagctca agttcgactc cgtccacctg cccacggaga cgccggccat
      721 caccgcgccg tagtgtcgac ccccgcaggg agcggcagct cccatccccg agcacattct
      781 ctccggcggc ccttcggggc cgccggtacg aggcgagaac ggaatgaagc ttgatttcgt
      841 cgtgaatcgc cggtcttcgt acagtccgtt gaatcccatc gggctgcgtg gccggatctg
      901 gcggtccctg ctggtgctgg ccctcctgcc gctgctggtg tcgcgcggcg tccccgccgt
      961 cgcccaagag gggggtatcg cgggcgctcc ggccgccgag gcgccgcgga tcgacgccgt
     1021 cgagttccgc ggcctcaagg ccttgaccga ggagacgctc ctctactacc tcgggctcga
     1081 ggtgggccag ccgctcgacc aggcgttgct cgaccagaac atcaagaccc tctgggagcg
     1141 cagcctgatc gacgacatcc aggtcgaggc gattccggcc gcgctccccg ccggcaacgc
     1201 ggtgcgcctg gtgatcaccg tcgccgagcg ccccgtcctg cgctccatcg agtacgaggg
     1261 gctcaagcgc atctcgaaga ccgatatcca ggacaagatc gccacccagc gcatccgcgt
     1321 gcgggagggc gagccgttga gcctgggcga gctgcagcgg gtcaaggcgc tgatcgagga
     1381 gctgcacgcc gagaagggct accgcttcgc ctccgcccgt tacaacgtcg aggacatcgg
     1441 caccaacgag aagcgcgtgg tgttcacggt cgacgagggc aaccgcgtgc gcatcgagga
     1501 catcgccttc gagggcaaca cggtctacaa cgacctgcgc ctgcggctgt ccatgcgcaa
     1561 gaccaaggag acgggcctga tctcccgctt ctccaagaag gacatctacg atccggccaa
     1621 gctccaggag gatctggaga aggtgcgcga cctctaccgc gccgccggct acaagaacgt
     1681 ggtcatcggc gagccgcaga tcgacgtgcg ggccctcaag cccgaggcgg ccgcgaccag
     1741 cgagcagaaa cggcgcatgt tcatcaccgt gcccatcgag gagggggacc gctggcgctt
     1801 cggcgagatc tcgatcgacg gcaacgacgt ctacaccgac caggcgctgc tccgcgtctt
     1861 cgcgcacgag cccaacggct ggctgcgctc caaggtgatc gacgacggcg tcaagtcggt
     1921 caccgacctc taccacaaca ccggctacgt ctatgcccag gtggagcccg agctggtgga
     1981 gaagccaggc cgcgtcgccg acctcatcgt ccacgtgcag gagggcgagc agttcacggt
     2041 cggccgcatc gagttccagg gcaacgagcg cacccaggac aaggtgctgc ggcgcgagat
     2101 ccggctctac gagagcggcg tggtcaacat cgcggcgatc cgcaacagcg tgctcaagat
     2161 caaccagctc ggctacttca agctcaacga ggaggacccg gtcgagttcg tcgccgacaa
     2221 cgaggacaag accgtcgacc tcatcttcaa ggggcaggag tcggagcgca ccgagctgca
     2281 gttcggcggc ggctggagcg aggtcgacga gttcttcggc cagttctcga tcaacaccaa
     2341 gaacttcctg ggccgcggcg agcaggtggg cgtctccctg cagcagggca agtaccgcaa
     2401 cttctacgat gtctcctact tcgtcccctg gttcatggac cgccgccaga gcatcggcct
     2461 gcgcgccttc gaccaggacc tcgactaccg gggcttcgac gcgcgggagc gctacgcgcg
     2521 ccagagccgc ggcgccatcc tcacctatgg ccgcagcttc cggctgttcc agtcagcgag
     2581 catcagctac aaccgctcgc gctacaacga ccagtcgacg gtgctggacg agttgacggg
     2641 caacccgatc gccctgccga ccctgagctt cgacaactcg tcgctgcggc cggcctatct
     2701 gttcgacagc cgggacaacc cgtacgagcc gacccgcggc aagcgcctgt cgctgtctgc
     2761 ggagtacgcc ggcggcttcc tcgggggcga cacctacttc ctgcggccgg agctggcgtt
     2821 cagcatgttc cagccggtgg gcagcctgcc ggtccgccag gtggtggcgc tcaacgtcga
     2881 ggccggcctg gtcgatccgt tcgaccccaa ggagtacccg ctgtcgccgc tcgacctctt
     2941 cttcctcggt ggcgagaaca gcatccgcgg cttctcgttc cgcggcatct cggtacgcaa
     3001 cgcggacggg acggtgcggc gcgacacctt cggcgcgatc gtcggcggcg acaagtacgc
     3061 ccaggtcaac ctggagtacc atttcctcct tggcggccca ttccgggtgc tcgtattcgc
     3121 ggatgccggc aacgtgtacg ccgacgacca atcgttcgat ctgtcgaacc tgcgctacac
     3181 cgccggtgcc gagctgcggg tcctggtgcc ggtgttcggc gcgccgctgc ggttcatcta
     3241 tgccctcaac ctggacccgt tgccgggcga cgatgacagg ttcgaaaatt tccagttcag
     3301 tatcggaacc agcttctaag acgtaaggag aagacccatg ttgattcgca gattccccaa
     3361 gaccctgctc gccctcgggc tggggctggc gttgtcggcg ccggccatgg cccaggggcc
     3421 gaagatcgcc gtcatcgaca ccgagcagat cctgctcgcg tcgaataccg gcaagaaggc
     3481 cctggccgac ctcaagaagc tccaggagca gaaggagacc gagggcaaga accggtcgca
     3541 ggagatcaag gacctgcaga ccaagctcaa cgacggccgc ctgtccctgg ccgccgacaa
     3601 gatcggcgac atggagaagc agctcgagga caaggtcatc gccctcaagc gcttccagga
     3661 cgacgccaac cgcgagctcg gcaagcggcg cgacgaaatc ctcgcctcgg tcgaccagaa
     3721 ggtcatgccg gtgatcaacc agctcggcaa ggagcagggg tacaccctga tcttccgcaa
     3781 gttcgagagc gggctcatct acgccgacga ggccgtggac atcaccggcc aggtcatcca
     3841 gcgcctcgac ggcggcggcg gcgcggccca gggaagcaag ggcaagtaag cgcgtgacgc
     3901 gccgcctcgc cgagctcgcg gagctggtcg ggggcacggt cgaaggggat ccggagcgct
     3961 ccatcgaagg ggtgcggacc ctcgacgcgg ccggcccccg cgatctctcc ttcctcaatc
     4021 atccgcggta ccgggagcag gcgtcggcca gccaggccgg cgccctgctc gtcccccgga
     4081 cgctcgccgg tctggccggc ctgcctggag acaccggcaa ggacctgctg gtcgtctccg
     4141 acgccgccta cgcgctttcc cgtctgatcg ccctcttcaa cgagcccgag gcgcgcgagc
     4201 cgggggtcca ccccacggcc gtgctggagc cggggtgcgc cgtcgacccg gccgcccacg
     4261 tcggtcccta tgtggtgatc ggcgccggca gccgggtcgc cgccggagcc gtgctccacg
     4321 cccacgtcgt cgtcgggcgg cgctgcgcgg tgggggagcg ggccgtcctc tacccccacg
     4381 tggtgctcta cgacggcacg gaggtcggcg ccggcggcat cgtccactcc ggtgccgtgc
     4441 tgggaagcga cggtttcggc tacgctaccg cgcgcggcgt gcaccacaag gtgcctcagg
     4501 tagggcgggt ggtggtgcac gaggacgtgg agatcggcgc caacaccacg atcgaccgcg
     4561 ccaccctggg cgagacggtc atcggcgccg ggaccaagat cgacaacctg gtccagatcg
     4621 gtcacaacgt gagggtcggc aggcactgta tcctgtgcgg ccaggcaggg atcgcgggga
     4681 gcacccatct tggggacggg gtggtgatgg caggccaggc gggcgccgcc ggtcacatcc
     4741 acctcggcga ccgggcgcag gtggcggcca agtcggccgc gctggccacg gtccccgcgg
     4801 gtacgacggt ggccggcatc ccggccgtgg agatgcgcaa gtggcggcgc caggcggtcc
     4861 tcgtctcgcg cctcgaggag atgagccgcc ggctccgggc tctcgagaga cggctgcacc
     4921 tcgagcacgg gagcggcgcg ccggaggcgc cggaggacat tggcgaggag gaatgagttg
     4981 agcgagagcg agccgacccc caagtgggac atcgagtgga tccagtcgat cctgccgcac
     5041 cggtacccga tcctgctggt cgaccgggtg ttggagatcg agccgaagaa gcggatcgtg
     5101 gcgatcaaga acgtgaccat caacgagccg ttcttcgccg gccacttccc gggccgcccg
     5161 gtcgtccccg gcgtgctgct ggtggagggg atggcgcagg ccggcggcat cctcctcctt
     5221 caggacatcc cggaccggga gaagaagctc gtctacttca tgggcatcga ggaggcgaag
     5281 ttccggcgcc cggtcgtccc cggcgaccag gtgcgctacg aggtcgaggt gctgcgcctg
     5341 cgcacggctt actgcaagct ggccggcaag atcatggtgg acggcaacct ggcggccgag
     5401 gcggtcatca cgtcggccat ggtgaaccgc tgagggcggc gcgatggcaa tgaccaccga
     5461 gctcattcat ccccaggccc tggtcgatcc cggcgccgtg ctgggcgagg gggtgcggat
     5521 cggccccttc gccgtcgtgg gccccggcgt ggtgctgggc gacggcaccg aggtcggagc
     5581 cggcgcccag gtccaggggc cggcgcgcat cggccgcgag aaccgcatct tcccctatgc
     5641 ctgcatcggc ttcgagccgc aggacctcaa gttccgcggc gaagaggtgt cggtggagat
     5701 cggcgaccgc aaccagttcc gggagttcac caccgtccac cgcggcacct ccaagggggg
     5761 cggcgtcacc cggatcggca gcgacaacct cttcatggcc tacacccacg tggcgcacga
     5821 ctgccaggtg gggagccgga cgatcttcgc caacaacgcg acgctcgccg ggcatgtcga
     5881 ggtgcacgac gacgccaaca tcagcgcctt cacctcggtg caccagttct gccgcgtcgg
     5941 ccgccacgcc tacatcggcg gctactcggt gatcaccatg gacgccctgc ccttcgtcaa
     6001 gacggtgggc atctcgccgg cgttctacgg cctcaactcc atcggcctga agcgcaaggg
     6061 gttctccccg gagggggtga agcgcctcga ccgggcggtg cgcatcctgg tgcgctcggg
     6121 gctcaacacc tcgcaggcgc tggagcggat gcgcgccgag ctggccggcg accccgacgt
     6181 cgattacctg atcgcgttcg tcgagggctc cagccggggc gtccacaagt cgatgccgcg
     6241 cggcggccgg tcgggaggcg ccgaggcgcc tccggaggcc tcttgagcgc gcccctgcag
     6301 tcactgcggg tcggcgtcgt cggcgtgggc gccatggggc gccaccacgt ccgcatcctg
     6361 tcgtcccttc ccggcgccga gctggtgggg atccacgacc cccgtatcga ggccgccgag
     6421 gcggtggccg ccgagcacgg cgcccgcgtc ttcacctctc tggacgagct ggccgggggg
     6481 atcgacgccg cggttctggc ggcgccgacc gtcacccacg ccgagaccgg ctgcgcgctg
     6541 ctggagcgcg ggctccacct gctggtggag aagcccatcg cctccagcct cgccgaggcc
     6601 gaccgcctga tcgcggcggc ggacggcgag ccggggaggc gacgggtgct ggccgtgggg
     6661 cacgtggagt tctacaaccc ggcggtgcag tcgctgctcg gtctgggggt gccgccgcgc
     6721 ttcgttgaga tccagcgcct cggcgtcttc tcgccgcgca gcctggacgt cgacgtggtg
     6781 ctcgacctga tgatccacga cctgcagatc ctccacggcc tggacccgag cccggtgcac
     6841 gaggtgcgcg cgacgggaat cgccgtgctg tcgccgcgga tcgacatcgc caatgcccgg
     6901 gtggagctcg cctcgggatg cgtggcgaac ctgaccgcct cgcgggtgtc ggccgagcgg
     6961 gtgcgcaagc tgcgggcttt cctgccgagc cgctactact ccctcgacta ccaggcgcag
     7021 gagatcaagg ggtaccgcct ggaggagggg gagggggcgc gccgcatcgc cccggacgac
     7081 ctgccggtgg agaaggggga gccgctgcgg cgggagctgg aggccttcgt cgccgcctgc
     7141 cggggtgaat ccgcccccct ggtgaccggc ggtgccggcc gccgcgccct ggagaccgcc
     7201 ctggcggtgg tggaggcgat cggctagccg ctggccgggc tgtccgcccc gcctcccgcc
     7261 tgctagactg tgcggccggg tcgccccggc atcccaatca aggagaggag ctttttcatg
     7321 agtgatatcc gtatcggtgt gatcggcggc agcggtctgt accagatgga gggcctcgtg
     7381 gtccgcgagg agcgccgtct ggagaccccg ttcggcgatc cgtcggacgt ctacgtcatc
     7441 ggcgagctgg acggccgccc ggtggccttc ctgccgcgcc acggccgcgg ccatcgtctg
     7501 ctgcccaccg agctcaacta ccgggccaac atcttcggct tcaagcagct cggcgtcgag
     7561 cagctcatct ccgtctccgc cgtcggctcg ctcaagatcg agtacaagcc gaccgacatc
     7621 gtcgtccccg accagttctt cgaccgcacc cgccaccggg tggacacctt cttcggcggc
     7681 ggcctggtgg cccacgtctc cctcgccaag ccggtctgcc cacgcctcat cggccacgcg
     7741 gcggaggcgg cccgggcggc cggcgccacc gtccaccagg gcggcaccta cgtgtgcatg
     7801 gaggggccgc agttctcgac ccgctccgag tccgagagct accgccgcga gggcttcgac
     7861 atcatcggca tgaccaacct gcaggaggcg cggctcgccc gcgaggccga gatctgctac
     7921 gtctcgctct ccatggtcac cgactacgac tgctggcacg agaccgagga ggccgtctcc
     7981 ggcgaggcgg tgatggaggt cgtgcgccag aacgccaaga tgtcccagga ggtcgtgcgg
     8041 cacatgctct cccggctggg ggatggccga gagcgcggct gtctgtgcga gagcgcgctg
     8101 gcgatgtcgc tgatcaccga gcgcgcggcg atcccggccg agacggtcca gaagctgcag
     8161 ccgatcatcg gcaggtacat caaggcttag atgacgcgca tcctggtcac caacgacgac
     8221 ggcatcttct cgcaggggat ccgcctcctc gcgtccgccc tctccgagct tggggaggtg
     8281 accgtggtgg cgccggaccg cgagcagagc gccatggggc acgccctgac cctctcccgc
     8341 ccgctacgca tgcagaaggt ggaggagaag tggtatgccg tggacggcac gcccacggat
     8401 tgcgtgaacc tcgcggtcct ctccctgctc caggagagcc cgccggacct ggtggtctcc
     8461 gggatcaact tcggcctcaa cctgggcgac gacgtgacct actccggcac ggtgagtgcg
     8521 acgttcgagg ggaccctgct ggggatccct tcggtcgcgt tcagccagga ggtcgcggag
     8581 ggcttctcgt tcgccccggc ggcgcgcttc gcccgcgacc tctgcgaggt gctgctggcg
     8641 gcgggggagc tcccccggga cctgctgctc aacgtcaacg tgccggccgg gccggtccag
     8701 ggggtgagct tcacccgtct ggggcggcgg atctacaagc agtctgtcat cgagaagctc
     8761 gacccgcgcg gccgcaagta ctactggatc gccggcacgc cgcagtggca gaaggcggag
     8821 gggaccgact acgaggcgat ctcccagaac cgcatctcgg tgacgccgct ccacctggac
     8881 ctcacctact accctggcct cgagagcttc ggctccttgt gggacaagct ctcggccctc
     8941 accttccata aacccgaatg agcgacttcg cggtccagcg cgagcggatg gtccgcgatc
     9001 acatcgccgc gcgcggcatc aaggacgagc gcgtgctggc ggcgctgcgc cgcattccgc
     9061 gccacctctt cgtgcgcgat catctgcgga gccgggccta cggcgaccac gcgctgccga
     9121 tcggcgcgtc gcagaccatc tcgcagccct acgtggtggc gaagatgagt gagatgctgg
     9181 aggtgggcaa ggagcactcg gtgctggaga tcggcaccgg ctccggatac cagaccgcca
     9241 tcctggccgt gctggcgcgg cgggtctaca gcctggagcg gatcgccgag ctggcccacc
     9301 aggcgatccc gcgtatgcgc gagctggggc tcgacaacgt taaggtccag gtgttcgacg
     9361 gcaccgtggg gtggagcgag tgggccccct atgaccgcat cctggtgacc gcggcggcgc
     9421 ccaaggtgcc ggaaccgctg ctcgagcagc tcgcgccggg cggcacgctg ctggtgcccg
     9481 aggggtcgat caacgcccag cggctcgtcc tctacaagaa gtcggcgcgc ggcgaggtgc
     9541 gtcgcaagga aggcgaggag gtggccttcg tgccgctcct cggccgccac ggctggaagg
     9601 cggacaagta gtgccggcgg tgcggatgct gctgagcggc cgggtgcagg gggtcggctt
     9661 ccgctggttc acccgcgcgg ccgcccagga gctgggcctc gccgggctgg tgcgcaatct
     9721 gccggacggc cgcgtcgagg tggaggtggc cggcggtccg gaggggctgg aggcgttccg
     9781 ccgccggctg cgggaggggc cgcccggggc gcgggtggag gcgctggagg agcaggagct
     9841 cgacaccgtc cccgcttggg acggattccg aatcgatcgc tagccggtgg gctggccgga
     9901 gggagcatga acgatttgaa gcagtacatc cgcgaggtcc ccgacttccc gaagccgggg
     9961 atcttgttct acgacatcac caccctgctg cagaacccgc tcgccctgcg catgacgg
//
LOCUS       DASPJI010000398         7502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_83310, whole genome shotgun sequence.
ACCESSION   DASPJI010000398 DASPJI010000000
VERSION     DASPJI010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7502)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7502
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83310"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2945)
                     /locus_tag="VEL74_10700"
     CDS             complement(<1..2945)
                     /locus_tag="VEL74_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYG63041.1"
                     /translation="MKSLNRQDATLIEVLRRRAAEMPDRPAYAFLADGEVEAERLTWA
                     ELEARVRTVAAALSAEVPRGERALLLYPPGLEFVAAFLGCLAAGIIAVPAYPPRPRRD
                     QPRLRSILADATPRIALTTAELAGGLPEMARISRLATDTLPVAPDWDGPLPDPESPAF
                     LQYTSGSTSLPKGVTITHANLVHNSGMIQRAFRQDAESVVVGWLPLYHDMGLIGNVLQ
                     PLWCGGRCVLMSPVAFLQRPRRWLEAIGRYRATTSGGPNFAYQLCAQKIGPAEREGLD
                     LSSWQVAFNGAEPVRAEALERFAAAFGPQGFRREAFYPCYGLAEATLFVSGATLGEPV
                     RVEAVDAAALERHEAVPAGEETARRLVSCGRAWDGQRIAIVDPESGAELPPGRVGEVW
                     VAGPSVAAGYWGRPEVTERDFQARLDLGDGPFLRTGDLGFLKDGELFVTGRLKDLIIL
                     RGRNHYPQDLERTAEAGHAELRPGGAAAFAVEVNGEERLVVAAEVERRWRAELEELAA
                     AVRRTVAEEHEVQVYEVVLLKPGGLPKTSSGKVQRHACRAGYLDGTLEALGTSAVGHG
                     GRRDEVPAKRPAPPREELAALPEAERREAVLAWLGDRVANLAGLGTRPAPDQPLTELG
                     LDSLSAVELKGEVEAAFGGTLPLAELLGGWGTGEVAERVLTPRPPLPSPSHTPAGRGG
                     EEGEYPLSIGQEALWFLERLAPTGGAYNIAVAARVAGELDGEALHRALLRLVERHPAL
                     RTTFQALEDGAPVQRVGPAAVDFAVAESEERWVQEAWRPFDLENGPLLRVRLLAGGLI
                     LFAVHHLVADFWSLAVASRELGALYREETGGAPADLPSLEGDYSGFVQRQREMLAGPR
                     EEELWSFWRETLAGPPPDLDLPTDHPRPTVPSFRGGARALVLPPELAERARSLARQSG
                     ATLFATLLAVWQTLLGRWTGQEDFAVGSPTVGRPDPALAGLVGYFVNPIPLRAELAGD
                     PPFAAHLER"
     gene            complement(2990..3652)
                     /locus_tag="VEL74_10705"
     CDS             complement(2990..3652)
                     /locus_tag="VEL74_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63042.1"
                     /translation="MAMAPLLFLAVAVLSLKTVWDLVPRAGSFPDWFVPTFAAGVTVL
                     VTVAGSTALGLWRARDFARPAGGRWALAVWVLLVLSFVCTSSVHQFHPFRLAPAAAYE
                     GISPVLHFNLVVTGVSFLAAVGLGVLYARQRRGVALAGLVMLGLVLLVPNDDCANPFN
                     DWWLATVGASPLMFLPNLYANLFGMAALMGVRPRFHLLALAATCAGVTLLGLGHMTRV
                     IW"
     gene            complement(3984..4241)
                     /locus_tag="VEL74_10710"
     CDS             complement(3984..4241)
                     /locus_tag="VEL74_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HYG63043.1"
                     /translation="MECRVGCGACCIALSISSPIPGMPEGKPAGVRCVQLTGDNLCKL
                     FGDRRRPVVCERLTPLEEMCGESRDEALVYLGWLERATAPR"
     gene            complement(4336..7227)
                     /locus_tag="VEL74_10715"
     CDS             complement(4336..7227)
                     /locus_tag="VEL74_10715"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HYG63044.1"
                     /translation="MRRFPLSVLALSILSIAALPFAGWADPPPAVVEEQGDEVPGLIA
                     PVRVFRDTHDIPHIFARNDRDALYALGQAHARDRFFQMDVLRRQFSGTLAELVGPPGL
                     ETDVQLRTLGLRRAAEASLAVLSRETRVWLNAYARGVNDYIYDTANPMPPEYAALELT
                     RSSLEPWTPVDSLVIAKGLAFGLSFDLSDIDLTVALGAFQQAGQAAGFNGTLLFSQDT
                     YRAAPFDPAVSIPGWLRGRTVSTIAGIRPEASGPKSATVPEYLTERTLDLARRYRDQA
                     RRVPLLRDALERRAAGNGSNWWVAGGSVTESGNPILANDPHLQLSVPSIFYEAQLRVS
                     GGAGPAMNAFGVTFPGIAGIVLGCNTRVCWGATTNPMDVTDVYQERLVIDPVLGLPTH
                     TLFDGRREPLVVIPQTFSVNRTGNGTMDDLADAGLGPLEGGITLVVPRRNNGPIVSVD
                     LSDPANATAISVQYTGWSATRELDTFRMWHRTADLDDFRAALQLFDVGSQNWSYADVD
                     GNIAYFTSAEMPLREDLQLLERPDGGIPPYLIRDGSHTLRHEWLPLRGRQPGQAIPFE
                     ILPFEEMPQEVNPERGWIANANNDPVGTSNDNDPLNQRRRAGGLFYLGIAYDRGYRIG
                     RIERLLEAELARDGSFSVEDFERMQANHQMLDAEVLVPHILNAYHRATRPAADGQLRD
                     LVSDIRVQQAVERFRNWDFSTPTGIREGYDPGDDPTALPEPSAEEIANSVAATLYSVW
                     RGQVLEKTIDRTLSRVGLGDFVPPGDVALADLRHLLESFGRTKGVGASGLQFFNAPGF
                     SAPADARDFIILESLLHALYILSGDSFAPAFNRSLDLNDYRWGRLHRLVLDHPLGGPF
                     DIPPGGGLSHLGPHLPGIARSGGFGVVDASNHDPRASSYDDFMFGSGPARRFIGEMTR
                     RGPEAEEVIPGGESGIPGSPFQTDQLRLWLTNRYHPWLWRPADVAGASR"
     gene            complement(7300..>7502)
                     /locus_tag="VEL74_10720"
     CDS             complement(7300..>7502)
                     /locus_tag="VEL74_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYG63045.1"
                     /translation="GVNADMPPQWIVYIIVEDLDHSVRQCVEMGGKLLRQPTSMGPHS
                     RYCVIQDPAGAVAALFESAKEG"
BASE COUNT         1175 a         2712 c         2647 g          968 t
ORIGIN      
        1 atccgctcca ggtgggcggc gaagggggga tcaccggcga gctcggcgcg gaggggtatg
       61 ggattgacga agtagcccac caggccagcc aaggccggat cgggccgtcc caccgtgggc
      121 gagccgacgg cgaagtcctc ctgcccggtc cagcgcccca ggagggtctg ccagacggcg
      181 agcagggtgg caaacagagt ggcgcccgac tgccgagcca gcgaccgtgc ccgctccgcc
      241 agctccggcg gaagcaccag agcccgagcg ccgccccgga agctcggaac cgtcggccgg
      301 ggatggtccg tgggcagatc gagatccggc ggcggtccgg ccagcgtctc gcgccagaag
      361 ctccacagct cctcttctct cggacccgcc agcatctccc gctgccgctg gacgaacccg
      421 gagtagtccc cctccagaga cggcaggtcc gcgggagccc cgccggtctc ctcccggtag
      481 agcgccccca gctcccggct cgccacggcc agggaccaga agtcggccac caggtgatgg
      541 acggcgaaga ggatcaggcc gcccgccagg agccggacgc gcagcagcgg cccgttctcc
      601 agatcgaacg gccgccacgc ctcctgcacc cagcgctcct cggactcggc caccgcgaag
      661 tccaccgccg ccgggcccac ccgctgcacg ggagcgccgt cctccagggc ctggaaggtc
      721 gtccgcaggg ccggatgccg ctcgacgagc cgcagcaggg cacggtgcaa cgcctcgccg
      781 tccagctctc ccgcgacccg cgccgccacc gcgatgttgt aggcaccacc cgtaggcgcc
      841 agccgctcca ggaaccagag cgcctcctgt ccgatggaga ggggatactc gccctcctct
      901 ccccctctcc cggcgggggt gtgggaggga gatgggagag ggggccgggg ggtgaggact
      961 cgctcggcca cctccccggt cccccacccg cccagcagct cggccagcgg cagcgtcccg
     1021 ccgaacgccg cctccacctc ccccttgagc tccacggccg agagcgaatc gaggcccagc
     1081 tctgtcagcg gctgatccgg cgccggacgg gtcccgagcc cggccagatt cgccacgcga
     1141 tctccaagcc aggcgagcac cgcctcccgc cgctccgcct ccggcaacgc cgccagctcc
     1201 tcccgcggcg gcgccggccg cttcgccggc acctcgtccc ggcgcccacc gtggccaaca
     1261 gcgctcgtcc ccagcgcctc cagagtgccg tccagataac ccgcccggca ggcgtggcgc
     1321 tgcaccttgc cgctggaggt cttgggcaga ccgcccggct tcagcagcac cacctcgtag
     1381 acttggacct catgctcctc ggccacggtc cgccgcaccg ccgcggccag ctcctccagc
     1441 tccgcgcgcc aacgccgctc cacctcggca gcgacgacca gccgctcctc gccgttcacc
     1501 tcgacggcga acgccgccgc gccgccgggc cgcagctccg cgtggcccgc ctcggccgtc
     1561 cgctccagat cctgggggta gtggttgcgg ccgcgcagga taatcaggtc cttgagacgg
     1621 ccggtgacga acagctcccc gtctttcaag aacccgaggt ctccggtgcg caggaacggc
     1681 ccgtccccca aatcgagccg ggcctggaag tcgcgctccg tcacctcggg ccgtccccaa
     1741 tagccggcag cgacgctggg cccggccacc cagacctccc ccactcgtcc cggcggcagc
     1801 tcggcaccgc tctcgggatc gacgatggcg atccgctgcc cgtcccaggc ccggccgcag
     1861 ctcaccagcc gccgcgccgt ctcctcccca gcgggtacgg cctcgtgtcg ctccagcgcg
     1921 gcggcgtcca ccgcctccac ccggaccggc tcgcccagcg tggcgccgga gacgaacagc
     1981 gtcgcctcgg ccaggccgta gcaggggtag aacgcctccc gccggaatcc ctgcggcccg
     2041 aacgccgcgg cgaagcgctc cagggcctcc gctcgcactg gctcggcacc gttgaaggcc
     2101 acttgccagc tcgacaggtc gagcccctcg cgctccgccg gcccgatttt ctgggcgcac
     2161 agctggtagg cgaagttggg gccccccgag gtggtcgccc ggtagcggcc gatggcctcc
     2221 agccaccgcc ggggccgctg caggaaggcc accggcgaca tcagcacgca ccggccgccg
     2281 caccacaggg gctgcagcac gttcccgatg agccccatgt cgtggtacag cggcagccac
     2341 cccaccacca ccgactccgc gtcctgccgg aaggcgcgct ggatcatccc ggagttgtgc
     2401 accaggttgg cgtgggtgat cgtcaccccc ttgggcaggg aggtggagcc cgacgtgtac
     2461 tgaaggaagg cgggggactc cgggtccggc agcgggccgt cccaatccgg agccacgggc
     2521 agagtatccg tggccaaccg ggagatcctg gccatctccg ggagcccacc cgccagctcc
     2581 gcggtggtca gggcgatccg gggcgtggca tccgccagga tcgagcggag gcgcggctga
     2641 tcacgccgcg gccggggggg atacgccggc accgcgatga tcccggcggc caggcacccg
     2701 aggaaggcgg cgacgaactc cagacccggc ggatagagca gcagcgcccg ctccccccgg
     2761 ggcacctcgg cggacagcgc cgcggccacg gttcggaccc gggcctccag ctccgcccag
     2821 gtcagccgct ccgcctccac ctccccgtcc gccaggaagg cataggcagg cctgtcgggc
     2881 atctccgcgg cccggcgccg gagcacctcg atcaacgtcg cgtcttgccg gttcagggac
     2941 ttcaagggcg gcacacctct aaggtgcgcc cgaccgggtt tccgcgcctc taccagatca
     3001 cccgcgtcat gtgccccagc ccgagcaggg tcacgccagc acaggtcgcg gcgagcgcca
     3061 gcaggtggaa gcgcggccgg acgcccatga gcgccgccat cccaaagagg ttcgcataga
     3121 ggttgggcag gaacatgagc ggcgacgcac cgaccgtcgc cagccaccag tcattgaacg
     3181 ggttggcgca gtcgtcgttg ggtaccagca gcaccagccc taacatcacg agcccggcga
     3241 gcgccacccc tcggcgctgc cgggcataca gcacacccag ccctaccgcc gcgaggaagc
     3301 tcacccccgt cacgaccaga ttgaagtgga gcaccgggct gatcccctcg taagccgccg
     3361 ccggcgccaa ccggaaagga tggaactggt ggacggacga ggtgcagacg aaggagagga
     3421 ccaggagaac ccagaccgcc agcgcccacc gtcccccggc gggccgggcg aagtcgcggg
     3481 cccgccagag ccccagggcc gttgatcccg cgacggtcac gaggacggtc acccccgccg
     3541 cgaacgtggg gacgaaccag tccggaaagc tccctgcccg ggggacgaga tcccagacgg
     3601 tcttgaggga caggaccgcg acggcgagaa agaggagggg ggccatcgcc atggagcgac
     3661 tctactcgct gtagatctcg tccggacagc ctggacgcga tcctgacggc ccgggaagct
     3721 tcccgaacgc cttggaatcc atcccattga attggacgcg atccggaagg gccgggaagc
     3781 ttcccgaacg ccgtggatct catccggacg gcctggacgc gatccgaaag gctcgggagc
     3841 ttcccggagc gtctggattc catccgcggc gtccgggcgg gatccggagc gcccgggaag
     3901 ctcaggagag gtccggatcg aagccgcggc atccgggcga ggaacacggc gttccggatg
     3961 cgctccatcg gcccgggaga aggctaccgc ggcgccgtgg cccgctccag ccagcccagg
     4021 tagaccaggg cctcgtcgcg gctctcgccg cacatctctt ccagcggcgt cagcctctcg
     4081 cacaccacgg gccggcgacg gtcgccgaac agcttgcaga ggttgtcgcc ggtgagctgg
     4141 acgcaacgga cgccggccgg tttgccttcc ggcatgccgg ggatgggcga cgagatggac
     4201 agcgcgatgc agcaggcgcc gcagcccacc cggcactcca cctacccggc cctccgcggg
     4261 aagaggagga ccccgaggac gaacagcagc accaccgggg tctttctcat ctcatgtctc
     4321 cagcgtcgtt gcagctcagc gcgacgcccc ggccacatcc gccgggcgcc acaaccaggg
     4381 atgataccgg ttcgtcagcc agagccggag ctggtcggtc tggaaggggc tccccgggat
     4441 gccgctctcg ccgcccggga tcacctcctc ggcctcgggg ccgcggcggg tcatctcgcc
     4501 gatgaagcgc cgcgccggac cgctgccgaa catgaagtcg tcataggagc tcgcccgcgg
     4561 gtcgtggttg gacgcgtcca ccaccccgaa gccgccggac cgggcgatcc ccggtagatg
     4621 ggggcccagg tgcgacaggc cgccgccggg cgggatgtcg aacggcccgc ccagcggatg
     4681 gtcgaggacg agccggtgga gccggcccca gcgatagtcg ttgaggtcga gagaccggtt
     4741 gaacgccggg gcgaaggagt cgccggacag gatatagagg gcgtgcagca ggctctccag
     4801 gatgatgaag tcgcgcgcgt ccgccggcgc cgagaacccc ggagcgttga agaactggag
     4861 acccgaggcg cccaccccct tcgtgcggcc gaagctctcc agcaggtgac gcaggtccgc
     4921 cagcgccacg tcgcccggcg gcacgaagtc gccgagcccg acgcgcgaca gggtccggtc
     4981 gatggtcttc tccagcacct ggccgcgcca gaccgagtag agggtggcgg cgacgctgtt
     5041 ggcgatctcc tcggccgaag gctcgggcag ggccgtgggg tcatccccgg ggtcgtaccc
     5101 ctcgcggatg cccgtgggag tgctgaaatc ccagttgcgg aaccgctcca ccgcctgctg
     5161 gacccggatg tccgacacca ggtcccggag ctggccgtcc gcggccgggc gggtggcacg
     5221 gtggtaggcg ttcaggatgt gcggcaccag gacctcggcg tccagcatct ggtggttggc
     5281 ctgcatgcgc tcgaagtcct cgaccgagaa gctgccatcc cgggccagct ctgcctcgag
     5341 gagccgctcg atgcggccga tgcggtagcc gcggtcgtag gcgatcccca gatagaagag
     5401 gccgccggcg cgccgccgct ggttcagcgg gtcgttgtcg ttgctggtgc ccaccgggtc
     5461 gttgttggcg ttggcgatcc agccgcgctc ggggttgacc tcctgcggca tctcctcgaa
     5521 cggcaggatc tcgaagggga tcgcctgccc aggctgccgg ccccggaggg gcagccactc
     5581 gtgccgcagg gtgtgcgagc cgtcgcggat caggtagggg gggatcccgc cgtcgggccg
     5641 ctccaggagc tggagatcct cgcgcagcgg catctcggcg ctggtgaagt aggcgatgtt
     5701 gccgtcgacg tcggcatagc tccagttctg ggagccgacg tcgaagagct gcagggcggc
     5761 ccggaagtcg tccaggtccg ccgtgcggtg ccacatccgg aaggtgtcca gctcccgcgt
     5821 cgcgctccag ccggtgtact ggaccgagat cgccgtggcg ttcgccgggt cggagaggtc
     5881 gaccgacacg atgggcccgt tgttgcgccg cgggaccacc agggtgatcc ccccttccag
     5941 cgggccgagg ccggcgtcgg ccaggtcgtc catcgtgccg ttgccggtcc ggttgacgga
     6001 gaaggtctgg gggatgacca ccagcggctc ccggcggccg tcgaagaggg tgtgggtcgg
     6061 caggcccagg accgggtcga tcaccagccg ctcctggtag acgtcggtga cgtccatggg
     6121 attggtggtg gcgccccagc agacgcgggt gttgcacccg aggacgatgc ccgcgatgcc
     6181 cgggaaggtg acgccgaagg cgttcatggc cggcccggcg ccgccggaga cacggagctg
     6241 ggcctcatag aagatgctgg ggacgctgag ctgcagatgg gggtcgttgg ccaggatcgg
     6301 attccccgac tcggtcacgc tgccgcccgc cacccaccaa ttgctgccgt tgccggcggc
     6361 gcgccgctcc agggcgtcgc ggagcagggg gacccgccgc gcctggtcgc ggtaacggcg
     6421 ggccaggtcc agcgtgcgtt cggtgaggta ctccggcacc gtggcgctct tcggacccga
     6481 ggcctcgggg cggatgccgg cgatggtgct gacagtgcgg ccgcgcagcc agccggggat
     6541 ggagacggcc gggtcgaagg gggcggcgcg gtaggtgtcc tggctgaaca ggagcgtgcc
     6601 gttgaagccg gccgcctggc ccgcctgctg gaaggcgccc agggcgaccg tgaggtcgat
     6661 gtccgacagg tcgaaggaga ggccgaaggc gagccccttg gcgatcacca ggctgtcgac
     6721 cggcgtccag ggctcgaggc tggagcgggt cagctccagg gccgcgtact cgggcggcat
     6781 cgggttggcg gtgtcataga tgtaatcgtt gaccccgcgg gcataggcgt tgagccagac
     6841 ccgcgtctcg cgggagagga cggcgagcga ggcttcggcg gcgcggcgca gccccagggt
     6901 gcggagctgg acgtcggtct cgagccccgg cggccccacc agctcggcga gggtgccgct
     6961 gaactggcgg cgcagcacgt ccatctggaa gaagcggtcc cgcgcgtgcg cctggcccag
     7021 ggcatagagg gcgtcgcggt cgttcctggc aaagatatgg gggatgtcat gggtgtcgcg
     7081 gaacacccgc accggcgcga tgagccccgg cacctcgtcc ccctgctcct ccaccacggc
     7141 cggaggcgga tcggcccacc ccgcgaaggg cagggccgca atgctgagga tggacagagc
     7201 gagcactgag agcgggaatc tcctcatgac atctcctttc ggcagaaacg gaaagaacca
     7261 atcggcgctt cagcttagcc gaaaggagat gcgcagggcc tacccctcct tcgccgattc
     7321 gaacagcgcc gcaacggcgc cggcggggtc ctggatgaca cagtagcgac tatgggggcc
     7381 catgctggtg ggctggcgca ggagcttgcc gcccatctcg acgcactgcc ggacgctgtg
     7441 gtcgagatcc tccacgatga tgtaaacgat ccattgcggc ggcatgtccg cgttcacgcc
     7501 cc
//
LOCUS       DASPJI010000399         4637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_768780, whole genome shotgun sequence.
ACCESSION   DASPJI010000399 DASPJI010000000
VERSION     DASPJI010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768780"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VEL74_10725"
     CDS             complement(<1..523)
                     /locus_tag="VEL74_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HYG63046.1"
                     /translation="MSEPAAHVTDAARPTLRRAVSRWQIVGLSLNDVIGSGVYLLPAE
                     AALILGPSSIWAVVLAGLAVLLLVLCFAEAASYFDRPGSAYLYARHAFGDLVGLEVGW
                     MTWLARVSSVASLSVGFARALGFLWPATRGGWGELLAICLPLLALTAINIVGVRSGVR
                     TAVFLAVTKIVPLL"
     gene            complement(551..1819)
                     /locus_tag="VEL74_10730"
     CDS             complement(551..1819)
                     /locus_tag="VEL74_10730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYG63047.1"
                     /translation="MVASPQPARLSLHERPVGAHRKILAFAWGAWCTGFYSLMLLSFL
                     LPPIQAELGLSEGSLARLTAVGVGMTGAGGLFFGWMSDRLGRRASLAASIAAFSIGNA
                     ACGLATGEGWLIVARALAGLGIGGTWGGGQALLGETFPPALRGRYGAIAQSGAPVGLG
                     LATIVGSFLAPELGWRTTFLLSALPGLILPFVPRLPESDLWLEHRRRLLEGHPDDVAH
                     ARRPILAQMVAPDLLSLFVRAFLLTAFNMSAYWFAVVWLPRYLQSRGLSIFGSGWWTL
                     TFVAGSLLGYLTFGWVSDAIGRRRAFALYCAVTAAGLAMVTLFWDAFASRPLVGLGCM
                     FIAGVGTGTWSCYGPYFSEIFPTRVRGATMSVIMNVTRGIQFVAPLVIAAVAGRWGLA
                     GGLALAAGFAVLAGLWVWTLPETAGARLEA"
     gene            2103..2990
                     /locus_tag="VEL74_10735"
     CDS             2103..2990
                     /locus_tag="VEL74_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HYG63048.1"
                     /translation="MRTRSLLSVLALLLPLTAAAQTGDVRPELLRLINQERARVGAPP
                     MRLSTPLGRAAQEHAGEIAGSGTFRLPGGSDKAMMARIERVGYEAAEWAENVTHTTAG
                     PQELMRDWKREAPNSYRQLMDPAFVDLGIGVSRLKGMPLYTLLFAVPRGEQFARATAG
                     LRDAERVRAAILREVNEVRRREGLRPLVLDPLLNQAAQKHAQDMLARSYFAHESPSGT
                     TVRERSRAQGYAWRSIGENIAEGQTTVDEVMQTWMDSPGHRKNILSPKFTELGVGLVV
                     GGRAGAYRVLWVQNFGSPR"
     gene            complement(3021..3863)
                     /locus_tag="VEL74_10740"
     CDS             complement(3021..3863)
                     /locus_tag="VEL74_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HYG63049.1"
                     /translation="MQKQAGFTARERFAALAAFPDRDIDIFEASLLIAAEEQPGLDPD
                     PWLRRLDLLSRELWLRLEGVEGDFDRLNQLTAFLFGELGLRGNDQDYYDPRNNFIDQV
                     LDRRLGIPLTLGIICMEVGRRVGIPLDGVGFPGHFLVRHSLHPNLILDPYEGGRLLTV
                     RDCEEILERVSGGKLTFEPRLLRPVGPRQILVRMLNNLRGIYLNQGEFGRALSVVERV
                     QLLIPEDPEVLRDHGFLSLQWGDPSQGIEELERYLDCEPEAEDQEEVRAVLAEARRRQ
                     AMPH"
     gene            3944..>4637
                     /locus_tag="VEL74_10745"
     CDS             3944..>4637
                     /locus_tag="VEL74_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HYG63050.1"
                     /translation="MIRRNPRLVLPALAAAMVLAAAAPQSARPPAPAAPRAAKPSPAP
                     RAAAPAAPRDQVLLRINEERTRTGAPPLAPSEALDRMARKRAAAIAAKGALPTEAESL
                     ALFNRLQQELIKEGYLAQGWTESVTITAGGAADVISYWKEDPSYREAMRHDYQDVGVG
                     VAEFSGIPLYTFLFAWPKSELYARQTAPLKDLQAVRESMLALVNARRKAEGRPPVNLD
                     PRLNEAAQKHAED"
BASE COUNT          756 a         1684 c         1645 g          552 t
ORIGIN      
        1 ccaggagcgg cacgatcttg gtcaccgcca ggaagaccgc ggtgcgcacg ccggacctca
       61 cccccacgat gttgatggcg gtcagggcga ggagcggcag gcagatggcg aggagctccc
      121 cccatccccc gcgcgtcgcc ggccagagga accccagggc gcgcgcgaag ccgaccgaca
      181 gcgaggcgac cgacgacacg cgggcgagcc aggtcatcca ccccacctcc aggcccacca
      241 ggtcgccgaa ggcgtgccgc gcgtagaggt aggcgctgcc cgggcggtcg aagtagctcg
      301 ccgcctccgc gaagcagagc actagcagga ggaccgccag gccggcgagc accaccgccc
      361 agatgctgga cggcccgagg atcagcgccg cctccgccgg cagcaggtag acgccgctgc
      421 cgatgacgtc gttcagggag agcccgacga tctgccagcg cgagacggcg cggcggaggg
      481 tcgggcgggc ggcgtcggtc acgtgagcgg caggctcgga catggatccc tttctctctc
      541 aggcgtcctg tcaggcttcg aggcgggcgc cggcggtctc ggggagcgtc cacacccaga
      601 ggccggcgag gacggcgaag ccggcggcca gggcgagccc tccggcgagc ccccagcgcc
      661 cggccacggc ggcgatgacc aggggggcga cgaactggat gccgcgggtg acgttcatga
      721 tcaccgacat ggtggcgccc ctgacccggg tggggaagat ctccgagaag tagggcccgt
      781 agcaggacca ggtcccggtc cccaccccgg cgatgaacat gcacccgagg ccgacgagcg
      841 gccgggaggc gaaggcgtcc cagaacaggg tgaccatggc gaggccggcg gcggtcaccg
      901 cgcagtacag cgcgaaggcc cggcggcggc cgatggcgtc ggacacccag ccgaaggtca
      961 agtagccgag gagcgagccg gcgacgaacg tcagcgtcca ccagccggag ccgaagatcg
     1021 acaggccgcg gctctgcagg tagcgcggca gccagaccac ggcgaaccag taagccgaca
     1081 tgttgaaggc ggtcagcagg aacgcgcgga cgaacagcga cagcaggtcc ggcgccacca
     1141 tctgcgccag gatcggccgc cgggcatggg ccacgtcgtc gggatgccct tccagcagcc
     1201 ggcgccggtg ctcgagccag aggtcggact cgggcagccg cggcacgaag gggaggatca
     1261 gccccggtag cgccgacaac aggaaagtcg tccgccatcc cagctcgggg gcgaggaagg
     1321 agccgacgat ggtggcgagg ccgagcccga ccggggcacc gctctgggcg atagcgccgt
     1381 agcggccgcg cagcgccggc gggaaggtct ccccgagcag cgcctgcccg cccccccagg
     1441 tgccgccgat ccccaggccg gcgagcgccc gcgccacgat cagccacccc tcccccgtcg
     1501 cgaggccgca cgccgcgttg ccgatggaga acgccgcgat cgacgccgcc agcgacgccc
     1561 ggcgccccag ccggtcgctc atccacccga agaagagccc gccggcgccg gtcatgccga
     1621 cgcccacggc ggtcagccgc gccaggctcc cctccgacag cccgagctcg gcctggatcg
     1681 gcggcagcag gaacgacagc aacatcagcg agtagaagcc ggtgcaccag gccccccacg
     1741 cgaaggcgag gatcttccgg tgagcaccga ccggacgctc atgcagggag agacgcgcgg
     1801 gctgcgggga cgcgaccaag gccgacaatc ctaccaggat cggggcggac ctcgcccgtc
     1861 gcgaaagcga ttcgaccctg ctcaaagttg gttcgaccct gctcaaagtt ggctcgaccc
     1921 tgctcagagt gggttcgatc ctgttcaaag ctggctcgac cccagggtcg agcctctcga
     1981 ccctgctcgg agctggtttg acccggttcg gagttggttc gacccgcggg tcgcgagccc
     2041 tccggcgagg gtcgaaccgc tccggaatgg cccctcacgc ggcctgtgtt gaaaggagcg
     2101 tcatgcgaac ccgttctctg ctgtccgtcc tcgccctcct cctccccctg accgccgccg
     2161 cccagaccgg cgacgtccgg ccggagctcc tgcggctgat caaccaggag cgggcgaggg
     2221 tgggggcgcc gccgatgcgc ctctccactc cgctgggccg ggcggcccag gagcacgccg
     2281 gggagatcgc cggcagcggc acgttccggc tgccgggcgg gtccgacaag gcgatgatgg
     2341 cgcggatcga acgcgtcggc tatgaggctg ccgagtgggc cgagaacgtc acccacacca
     2401 cggccggccc gcaggagctg atgcgcgact ggaagcggga ggcccccaac tcctaccggc
     2461 agctcatgga cccggcgttc gtggacctgg ggatcggcgt ctcgcggttg aagggcatgc
     2521 cgctgtacac cctcctcttc gccgtccccc ggggggagca gttcgcccgc gccaccgccg
     2581 gcctgcggga cgccgagcgg gtgcgcgccg ccatcctgcg ggaggtcaac gaggtgcgcc
     2641 ggcgcgaggg gctccgcccc ctggtgctcg acccgctgct caaccaggcg gcccagaagc
     2701 acgcccagga catgctggcg cgctcctact tcgcgcatga gagcccgagc ggcacgacgg
     2761 tgcgcgagcg ctccagggcc cagggctacg cctggcggtc gatcggcgag aacatcgccg
     2821 aggggcagac gacggtggac gaggtgatgc agacctggat ggacagcccg gggcaccgga
     2881 agaacatcct gtctcccaaa ttcaccgagc tgggcgtggg gctggtggtc ggcgggcggg
     2941 cgggggccta ccgtgtcctg tgggtccaga acttcggcag cccccgctga ccgcggcgcg
     3001 ggggtgatct ctcgatgacc tcagtggggc atcgcctgcc ggcgccgggc ctcggcgagc
     3061 accgcccgga cctcttcctg gtcctcggcc tcgggctcgc agtcgagata ccgctccagc
     3121 tcctcgatcc cctgggaggg atcgccccac tgcagcgaca ggaacccgtg gtcgcgcagc
     3181 acctcggggt cctccgggat cagcaattgg acccgctcga ccaccgacag cgcgcggccg
     3241 aactccccct ggttgaggta gatgccccgc aggttgttga gcatccgcac caggatctgg
     3301 cgcggcccca ccggccgcag cagccgcggc tcgaaggtca gcttgccgcc gctcacccgc
     3361 tccaggatct cctcgcagtc gcggacggtg agcagccgcc ccccctcgta ggggtcgagg
     3421 atcaggttcg gatgcaggct gtggcgcacc aggaagtgcc ccgggaagcc caccccgtcg
     3481 agagggatgc cgacccgccg gcccacctcc atgcagatga tcccgagcgt cagggggatc
     3541 cccaggcggc ggtcgagcac ctggtcgatg aaattgttgc gggggtcgta gtagtcctgg
     3601 tcgttgcccc gcagccccag ctcgccgaag aggaaggcgg tgagctggtt gagccggtcg
     3661 aagtccccct ccaccccttc gaggcggagc cagagctccc gggacaggag atcgaggcgc
     3721 cgcagccagg ggtcgggatc gagccccggc tgctcctcgg cggcgatcag cagcgacgcc
     3781 tcgaagatgt cgatgtcgcg gtcgggaaat gccgcgagcg ccgcgaaccg ctcgcgcgcg
     3841 gtgaagccag cctgtttttg catcctggac gcattataga cgatgacgca tgcttccgca
     3901 cccctttttc ggcggcgggt gcgctatcgt tcgaggtaga cccatgatcc gcaggaatcc
     3961 gagactcgtc ctcccggcgc tcgccgccgc gatggtcctg gcggcggcgg ctccccagag
     4021 cgccaggccc ccggccccgg ccgcgccccg ggctgcgaag ccgtcccccg cgcccagagc
     4081 ggcggcgccg gcggccccgc gcgaccaggt cctcctccgc atcaacgagg agaggacccg
     4141 gaccggcgcg ccccccctgg ccccctccga ggcgctcgac cggatggccc ggaagcgggc
     4201 cgcggccatc gccgccaagg gggccctgcc caccgaggcg gagagcctgg cgctgttcaa
     4261 ccggctgcag caggagctca tcaaggaggg ctacctcgcc caggggtgga ccgagagcgt
     4321 caccatcacg gcgggcggcg cggccgacgt catctcctac tggaaggagg acccgagcta
     4381 ccgcgaggcc atgcgccacg attatcagga cgtcggtgtc ggcgtcgccg agttctccgg
     4441 catccccctc tacaccttcc tcttcgcctg gcccaagagc gagctctacg cccggcagac
     4501 ggcgccgctc aaggacctgc aggcggtacg cgaatccatg ctcgccctgg tcaacgcccg
     4561 ccgcaaggcc gagggccggc cgccggtgaa cctcgacccg cgcctcaacg aggcggcgca
     4621 gaagcacgcc gaggaca
//
LOCUS       DASPJI010000400         6012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_319840, whole genome shotgun sequence.
ACCESSION   DASPJI010000400 DASPJI010000000
VERSION     DASPJI010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319840"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VEL74_10750"
     CDS             complement(<1..537)
                     /locus_tag="VEL74_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG63051.1"
                     /translation="MNILIIDDEEVLQDVLTFLLRKEGHTPFSAMSGEEGLEVLAREE
                     VDLVLLDLMLPGMHGMDVLREIRRKHPEQVVVVITAFSSIESAIQAMREGAFHYIPKP
                     FKNEEVLLTVRKGLEQRSLRAENRSLREQLRQRFAFDNIVGKSKPMQQVYELIQLAAP
                     AKSNMLILGESGTGKELVA"
     gene            complement(548..3355)
                     /locus_tag="VEL74_10755"
     CDS             complement(548..3355)
                     /locus_tag="VEL74_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG63052.1"
                     /translation="MAHARTLRLTSSLVLALLVATLCGVSVHRKVQTFQPLGFEPVIV
                     EGGGGLAVGAVTDAGTGLVQGDLIMMVNGGEVGNPAAMAERLSQQGESELTLLRADQL
                     QVIEYHRPPLAIDFSYLILALVGVGYLLIGLYMLLRQGGAQGMLFYLWCLASAAVYML
                     TRVPPLDPIFVALDLGDKLARIFLPALTLHLFLVFPSPLETGRLSRRLAPFLYLPAAA
                     LLALQLDLALANGRWIFGRPTVARLEALDRVDLLHLGVFALAALGILVYRMARSHGWE
                     QQRQLQWVAFGLGGGYLPFLLLYVVPRVLEVRLPMPQVLTPLAVLPLALVPLTFAYAI
                     LRYKLWDIEIIVRDTISSTLTLLLGIIGFSLVNLAITRGVSQELVVARNLLSFVAGLA
                     IAGLLVPTRRHLGSALERLQYRGTFGKRRALSAMGRELLHQRNLSQLCQVLVQDIQDG
                     VSLQRAALYLCQKGELVPFSADDPELPRGLACDSLGEELWQEDYRRFSGVALPCEPMS
                     AEQRLFVAGYRYAFPMTVRGRGVGLALAGYRQDQTPLNSEDLDLIRHLLDQASLAIEN
                     AQLMGQLHQQLEEVQRLQSYTEGIFESSPAGTAVLDRDGRLVSVNAAFAALAGRATRE
                     SLLGERLEDVLPLGTLPAPGEMPVQVSYMDGDATIGGGRRERFLQVSVADFERGRGLR
                     ILMVYDVTERVAMENALREKDRLAALGMLAAGVAHEVNTPITGISSYAQMLLAEIPET
                     DPHHAILKKVERQTFRASRIVNNLLEFARNRQKDRQHVAVAPLVSECLDLLSDRLQKR
                     QIRLEWAPPQEEILVLGCDGELQQVIANLVLNALDAMTETGGGTLGLEVEGDAATVRL
                     RVRDTGPGIPPEKLETIFQPFYSTKLNRGGTGLGLSISHEIARRHGGDLRVESEPGRG
                     ACFILELPRAVPGARSLPQPA"
     gene            complement(3634..5535)
                     /locus_tag="VEL74_10760"
     CDS             complement(3634..5535)
                     /locus_tag="VEL74_10760"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYG63053.1"
                     /translation="MENIRKTVPLLLAIALLGSGSVRGEGRQTGVEGQPGVEGRVHGR
                     TAPIASAGVYAYELADLSLRKVKTDAQGNFRFSDLPAGLYKVIAHKSGFLPVVVELTR
                     TSAQTYQRLELELAARSPSKASGLARDDFWALRAQVPSDVLRSIQMDQDLRLTSFEPV
                     AAGDSLANFHTHMEAMTGVAQAGSAGDGQMADGRLGIAGKVGPVQVDISGRFTQFNPG
                     NLAPSGAGGQARALSLSLEAGGSSRLHLTSLNNRLVNPNDESAPVDLEHYQVSWTQDV
                     GQNGRSDFAAVYTTESNYHRLGWLDPVDIPEASTTWRVEGAYTTSLGDRSTLQTGLRY
                     RERQFGLGARGHRAEEPGQSSVDLFSRGGLQVRPAILVEYGLYSTLSDGSLSLTPQGG
                     VVFQLGQNWQAAASASRRVYQDPVRYPDFLPALYEEGDLCEQGSEACYQVRLTRKLGD
                     ENEVSLSALHRTVGETLRLYFSDQLMDRLESLYLVRGDELPELQFTVTRRLSPQVLTT
                     LQSSVASGGGGTFVSAGQQPYENRVRYMVTSLDTHFQSTSTGVFVAFHQLQQDLEPIG
                     FSPLRKASLEHERLRLMLSQDLNILLDLAAQWAVQLNMELSRGPEQSSSQDADDVHRR
                     FMGGIAVKF"
     gene            complement(5627..>6012)
                     /locus_tag="VEL74_10765"
     CDS             complement(5627..>6012)
                     /locus_tag="VEL74_10765"
                     /inference="COORDINATES: protein motif:HMM:NF018534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HYG63054.1"
                     /translation="PHLFRMLTVYPAELVAWWRAQGNPVEDLPRLAPGCRGIPGGEPP
                     RIVSPDASTPYRLRRDAPPEHQRIPLIARAGPGTSRLFWYQDGLLVAATAPGESRFLD
                     PSPGEHRLVVADDLGRSDGMTYTIE"
BASE COUNT          949 a         2035 c         2136 g          892 t
ORIGIN      
        1 cgccaccagc tccttgccgg tgccgctctc gccgaggatc agcatgttgc tcttcgccgg
       61 cgcggcgagc tggatcagct cgtagacctg ctgcatcggc ttgctcttgc cgacgatgtt
      121 gtcgaacgcg aagcgctggc ggagctgctc gcgcagcgac cggttctccg cccgcagcga
      181 ccgctgctcc agccccttgc ggacggtgag cagcacctct tcgttcttga acggcttggg
      241 gatgtagtgg aacgccccct cgcgcatcgc ctggatggcg ctctcgatcg aggagaaggc
      301 ggtgatgacc accaccacct gctcggggtg cttccggcgg atctcgcgca gcacgtccat
      361 gccgtgcatg cccggcagca tgaggtcgag gagcaccagg tccacctcct cgcgggcgag
      421 gacctccagc ccctcctcgc ccgacatggc cgagaacggg gtgtgcccct ctttgcgcag
      481 gagaaaggtc aggacgtcct ggaggacctc ctcgtcatcg atgatgagga tgttcatgca
      541 ggtgcgttca tgccggctgg ggcagcgagc gggcacccgg caccgcccgg ggcagctcga
      601 ggatgaagca ggcgccccgg cccggctcgc tctcgacgcg cagatcgccc ccgtgccgcc
      661 gcgcgatctc gtggctgatg gacaggccca gcccggtgcc gccgcggttc agcttggtcg
      721 agtagaacgg ctggaagatc gtctccagct tctcgggcgg gatccccggg ccggtgtcgc
      781 gcacccgcag gcggaccgtc gcagcgtccc cctcgacctc gaggccgagg gtgccgccgc
      841 cggtctcggt catcgcgtcc agcgcgttga ggacgaggtt ggcgatgacc tgctgcagct
      901 cgccgtcgca gccgagcacc aggatctcct cctgcggcgg agcccattcc aaccggattt
      961 gccgcttctg cagccggtcg gagagcaggt ccaggcactc gctcaccagc ggcgccaccg
     1021 cgacgtgctg ccggtccttc tggcggttac gggcgaactc cagcaggttg ttgacgatcc
     1081 gcgaggcacg gaaggtctgc cgctccactt tcttgaggat ggcgtggtgg gggtcggtct
     1141 ccgggatctc ggccagcagc atctgcgcgt agctcgagat gccggtgatc ggggtgttca
     1201 cctcgtgcgc cacgccggcg gccagcatgc ccagggcggc caggcggtcc ttctcccgca
     1261 gggcgttctc catcgccacc cgctcggtga cgtcgtacac catgaggatc cgcagccccc
     1321 gcccgcgctc gaagtcggcg acgctcacct ggaggaagcg ctcgcgccgt cccccgccga
     1381 tcgtggcgtc accgtccatg tagctcacct ggaccggcat ctcgcccgga gcgggcagcg
     1441 tccccagcgg cagcacgtcc tccagccgct cgccgagaag ggattcgcga gtggcgcggc
     1501 cggcgagggc ggcgaaggcc gcgttcaccg ataccagccg gccgtcgcgg tcgagcaccg
     1561 cggtgccggc gggagaggac tcgaagatcc cttcggtgta gctctgcagc cgctgcacct
     1621 cttccagctg ctggtgcagc tgccccatga gctgggcgtt ctcgatcgcc agcgacgcct
     1681 ggtccagcag gtgccggatg aggtccaggt cctcgctgtt gagcggcgtc tggtcctggc
     1741 gatagccggc gagggcgagg ccgacgccgc ggccgcgcac cgtcattggg aaagcgtacc
     1801 ggtagccggc gacgaacagc cgctgctcgg ccgacatcgg ctcgcacggc agcgccacgc
     1861 ccgagaagcg ccggtaatcc tcctgccaga gctcctcgcc cagggagtcg caggccagcc
     1921 cccgcggcag ctccgggtcg tcggccgaga acggcaccag ctcgcccttc tggcagaggt
     1981 agagggcggc gcgctgcagg ctcaccccgt cctggatgtc ctggaccagg acctggcaga
     2041 gctggctgag gttgcgctgg tggagcagct cgcggcccat cgccgacagc gcccggcgct
     2101 tgccgaacgt gccgcggtac tggagacgct ccagggcgga gccgagatgg cggcgcgtcg
     2161 gcaccaggag gccggcgatg gcgaggccgg cgacgaagga gaggaggttc cgcgccacca
     2221 ccagctcctg cgacaccccg cgcgtgatcg ccaggttcac cagcgagaag ccgatgatgc
     2281 ccagcagcag cgtcagggtc gaggagatcg tgtcgcggac gatgatctcg atgtcccaca
     2341 gcttgtagcg caggatggcg taggcgaagg tcagcggcac cagcgccagc ggcagcaccg
     2401 ccagcggggt cagcacctgc ggcatcggca gccgcacctc cagcacccgg ggcaccacgt
     2461 agaggagcag gaacggcagg tagccgccgc cgaggccgaa ggccacccac tgcaactggc
     2521 gctgctgctc ccagccatgg gagcgggcca tgcggtagac caggatgccc agcgcggcca
     2581 gggcgaacac ccccaggtgg aggaggtcga cgcggtcgag ggcctcgagc cgcgccacgg
     2641 tgggccggcc gaagatccag cgcccgttgg ccagggcgag gtccagctgc agggcgagca
     2701 gcgccgcagc tggcaggtag aggaacggcg ccagccgccg gctcaaccgt ccggtctcga
     2761 gcggggacgg gaacaccagg aagaggtgca acgtcagcgc cggcaggaag atgcgggcga
     2821 gcttgtcccc cagatcgagg gccacgaaga tcgggtcgag cggcggcacc cgcgtcagca
     2881 tgtagacggc ggccgaggcc aggcaccaga ggtagaagag catcccctgg gctccgccct
     2941 ggcggagcag catgtacagg ccgatcagca ggtagccgac gccgacgagc gccaggatca
     3001 gataggagaa gtcgatcgcg aggggcggcc ggtggtactc gatcacctgg agctggtcgg
     3061 cccgcagcag ggtcagctcg ctctcaccct gctggctcaa ccgctcggcc atcgcagcgg
     3121 ggttccccac ctcgccgccg ttcaccatca tgatcaggtc accctggacc agacccgtgc
     3181 cggcgtcggt cacagccccg accgccagac cgcctccgcc ctccacgatc accggctcga
     3241 aacccagcgg ctggaaggtc tggaccttgc ggtgcacgct cacgccgcac agcgttgcga
     3301 cgagcaacgc caggaccagc gaagaggtca gtcggagggt gcgggcgtgt gccatggaga
     3361 acgtactcgg ccgtctttcg gcaaaagcac tttccatgcc ataccagcaa gtttttgaac
     3421 cgcctctaag tccttgtttt tgagtgtttt ttgccgctgc cgtccaaaaa accggtggcg
     3481 attcaatttt ttgagttcaa tggcttgggg cgggtgtgcc accttggcgg gggcccgggg
     3541 tcgtcctgcc cggctccttc gggtacgccc accatcccac ccctaaacag gggaggtggg
     3601 aggtgggcca ggatggggag aggggagtcc ggatcagaac ttgacggcga tgccacccat
     3661 gaaacgccgg tgcacgtcgt cggcgtcctg ggaggaggac tgctcggggc cgcggctcag
     3721 ctccatgttg agctgcaccg cccactgggc cgccaggtcg agcaggatgt tgagatcctg
     3781 gctcagcatc aggcggaggc gctcgtgctc cagggaagcc ttgcggagcg gcgagaagcc
     3841 gatgggctcc agatcctgct ggagctggtg gaaggccacg aacacgccgg tggaggtgct
     3901 ctggaagtgg gtgtcgagcg acgtcaccat gtagcgcacc cggttctcat agggctgctg
     3961 gccggcggag acgaacgtgc cgccgccgcc ggaggcgacg ctggactgca gcgtggtgag
     4021 cacctgcggg gaaagccggc gggtgacggt gaactgcagc tccgggagct cgtcgccgcg
     4081 caccaggtag aggctctcca ggcggtccat gagctggtcg ctgaaataga gccggagggt
     4141 ctcgcccacc gtgcggtgca gggcgctcag cgacacctcg ttctcgtcgc ccagcttgcg
     4201 ggtgaggcgg acctggtagc aggcctcgct gccctgctca cagaggtccc cctcctcgta
     4261 gagggcgggg aggaagtccg gatagcgcac ggggtcctga tagacccgtc ggctggccga
     4321 cgcggccgcc tgccagttct ggccgagctg gaacacgacg ccgccctgcg gggtgagcga
     4381 caggctgccg tccgacagcg tgctgtagag gccgtactcg accaggatcg ccggccggac
     4441 ctgcaggccg ccgcggctga acaggtcgac actcgactgc ccgggctcct cggcgcggtg
     4501 cccccgggcg ccgagcccga actggcgctc gcgataacgc aggccggtct ggagcgtgct
     4561 gcggtcgccg agagacgtag tgtaggcgcc ctcgacccgc caggtggtgg aggcctcggg
     4621 gatgtccacc gggtccagcc agccgagacg gtggtagttg ctctcggtgg tgtacaccgc
     4681 ggcgaagtcg gagcggccgt tctggcccac gtcctgggtc cagctcacct ggtagtgctc
     4741 gaggtcgacg ggcgcgctct cgtcgttcgg gttgaccagc cggttgttga gggaggtgag
     4801 gtggaggcgg ctcgagccgc cggcttcgag gctcagggag agggcccgcg cctggccgcc
     4861 ggcgccggac ggcgccagat tgcccgggtt gaactgcgtg aagcgcccgg agatgtccac
     4921 ctggaccggg cccaccttgc ccgcgatccc cagccggccg tccgccatct gcccgtcgcc
     4981 cgcggacccg gcctgagcca ccccggtcat cgcctccatg tgggtatgga agttcgcgag
     5041 gctgtctccg gcggccaccg gctcgaagct cgtcaggcgg agatcctggt ccatctggat
     5101 gctgcgcagc acgtcggagg ggacctgggc gcgcagggcc cagaagtcgt cccgcgccag
     5161 gccggacgcc ttggaggggg accgcgcggc cagctccagc tccaggcgct ggtaggtctg
     5221 ggccgaggtg cgcgtcagct ccaccacgac cggcaggaac cccgacttgt gggcgatgac
     5281 cttgtagagg ccggcgggga gatccgagaa ccggaagttc ccctgcgcgt ccgtcttgac
     5341 cttgcgcagg ctgagatcgg ccagctcgta ggcgtagacg ccggccgagg cgatcggggc
     5401 ggtgcggccg tggaccctgc cttcgacgcc gggctggccc tcgacgcccg tctggcgccc
     5461 ttcgccccgc acgctgccgg aacccagcag cgcgatggcg agaagcagcg gaacagtctt
     5521 gcggatgttc tccatccctc ttacctcaac gagccacttt actcccgcat cgttgacctg
     5581 tcaatggaca agaatcagtg cacccctgac cagtttttga gcactttcac tcaattgtat
     5641 aagtcatccc atcggaccgc ccgagatcat cggcgaccac caggcggtgc tcgccgggac
     5701 tgggatcgag aaagcggctc tctcccggcg cggtcgccgc caccagcagt ccgtcctgat
     5761 accagaacag gcgcgaggtc cccggaccgg cgcgggcgat cagcgggatg cgctggtgct
     5821 cgggcggagc gtcgcgccgc agccggtagg gggtagaggc gtccggcgag acgatgcgcg
     5881 gcggctcgcc cccggggata ccgcggcacc caggggccag acgggggaga tcctccaccg
     5941 ggttgccctg ggcgcgccac caggcgacca gctccgccgg gtagaccgtc aacatgcgga
     6001 agaggtgggg gc
//
LOCUS       DASPJI010000401         7143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_427202, whole genome shotgun sequence.
ACCESSION   DASPJI010000401 DASPJI010000000
VERSION     DASPJI010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427202"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>7143)
                     /locus_tag="VEL74_10770"
     CDS             complement(<1..>7143)
                     /locus_tag="VEL74_10770"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63055.1"
                     /translation="DALGRALAEVVRRHEVLRSTIRTEEGQAVQALAPAGPFPLPVID
                     LERLPGEARQAEARRLVDEEARRPFDLAAGPLLRAAALRLGAEEHVLVLLQHHIVSDG
                     WSMGILVREVVTLYEAFAAGRPSPLPDLPIQYADFAAWQHRRLQGEALDELLGWWRER
                     LAGAPDLLDLPTDRPRPAVQGWAGSTLSFVLPSGVRDGLDALARREGATLFMVLLAAF
                     AIVLQRYSGRDDVVVGSPIANRNRDEVEALIGFFVNTLALRADLSGNPTFRELLVRVR
                     EATLGAYDHQDIPFEMLVDELGLSRQVSHTPLFQVTFALQNAPAPAVEVRGLSLAPLP
                     APTGTSKFDLTLSMTEMPGGLAGSIDYSTDLFDRTTVERLAGHFGTLLEEIARDPDRR
                     LSELPLLSAEERRQATAWSAATARFPVAGSLHRRFEEQAAQAPQAEAVTGDGERITYA
                     ELDARAGRLAGRLRALGVGPESRVCLLAERSPALVVGVLGILKAGGAYVPLDPAHPDE
                     RLAFMARDSGAALLVAGPGFEERLAALGNGLQVVALEGEMAIGEELPDAAPDQAAYVI
                     YTSGSTGRPKGVVVTHANVLRLFAATDAWFGFGPDDVWTLFHSYAFDFSVWEIWGALL
                     HGGRLVAVPYLVSRSPDHFLDLLVRERVTVLNQTPSAFRQLVQAEGARGEAAGLALRW
                     VVFGGEALDIPSLAPWFNRHGDERPVLVNMYGITETTVHVTYRPMRRADLAAGRSLIG
                     QAIPDLSLHVLDLAGSPAPVGVPGEICVGGAGLARGYLGRPELTAQRFVPDPSGGEPG
                     ARLYRSGDLARRRPDGDLEYLGRIDQQVKIRGFRIELGEIEAAVAERPEVRQAVVLPS
                     EGGGETRLVAYVVPAGEALSTATLREDLRRRLPDYMVPAVWVVLDELPLTANGKLDRR
                     ALPAPGPARRDLDEEFAVPSTPTEELLAAVWAQVLGVDRVGVRDNFFALGGDSIRSIQ
                     VISLARERGLDFNLQQLFQHQTVEALAAELAPATEGATAVLKSEPFSLVSAADRALLP
                     EDVEDAYPLAALQAGMLFHMVLSREDAPYHNIDSWLLEARFETEVFQRAVDTVVARHA
                     VLRTSFHLEGFSEPLQLVHREAVLPVAEEDLRHLSRDAQEAVIDTFIEAEKRQLHDLR
                     QAPQLRIAVHRRSDATFQLTIAENHAIFDGWSLHSTLAELFELYFALLAGTPPREEPA
                     PAVTYRDFVRIERETVASEEARRFWEQALAGVEITEIPRWPVPHRAGGRRTRTLDAVT
                     TPEVGTGIHRLARIAAVPLKSVLLAAHLRVLALASGRSDVVTGLVTNGRPEVPGGDHL
                     RGLFLNTLPLRLRLEGGTWKDLARQAFTAEWEQLPYRRYPLALLQQARGGRPLFEVMF
                     NYIHFYVVDDLLGTGDVQVLGMKRLEGTNYPLQVALQPRSSGQQVGVAMEYDGEALHT
                     EQVRAMSGWFQRVLAAMAADPDGRYETIGLLTEAERHQLLLEWNDTRVDFPRDLLLHH
                     AIAAQAARTPDAVAVVFEEASLSYAELARRAGELAGRLRRLGVGPEARVGIAVERSLE
                     MLVGLLGILEAGGAYVPIDPSYPAERLEMMLEDARLPVLLTQERLLAALPLPAGGTAG
                     PFRVIALDVPPPDPEPPVGPAAPAVPVVGAGPGQLAYVIFTSGSTGRPKGAMNSHAGI
                     VNRLLWMQRTYGLTPADRILQKTPFSFDVSVWELFWPLLTGACLVVARPGGHQDAAYL
                     VETVLEREITTLHFVPSMMRLFLEERGLGRCAALRCVIASGEALPPDLRQRFFERLGR
                     SVELHNLYGPTEAAVDVTFQPCDIGGNGGELSIVPIGRPVANTRIHLLDEFLQPVPVG
                     TPGELYIGGVQVGRGYHDRPGLTAERFVPDPFGEPGARLYRTGDLARRLPDGPVDFLG
                     RMDHQVKVRGFRIELGEVEAALTLHPGIREAAVLAHTDGGEVRLTGYVVPRLEVGDLT
                     PANLREHLLARLPEPMVPALWVFLGEMPLSPSGKLDRRRLPAPDIALRSEAEYVAPRD
                     AMEQELAAIWSRLLGVERVGIHDNFFALGGHSLLAHRVLAAVREELQAEIELRALFEN
                     STVAELAAVISGVQAPAGSNRIPRQPRTPGEPGRFPVSFSQLREWILHQIEPGSSAYN
                     IPGGVLARGPLSIPRIIAAAQEIVGRHETLRTYFAAESGGEPVQVVVPRLEIPVPVVD
                     LNGLPEPLRDPVARQLAQENLRQPFDLARGTLLRLLVVRISPSDHLVHFTMHHIISDG
                     WSMSVFSRELTALYAALSAGQAPPLPELPVQYADFAVWQREQLQGGRLESHLEYWRRQ
                     LDGLPPLMRLPTDRPRPPVQTYRGARQTLFISREESAAIRALAVREGTSPFVVLLAAF
                     QALLGRWSHEEDLAVGT"
BASE COUNT         1145 a         2441 c         2618 g          939 t
ORIGIN      
        1 ggtgccgacg gcgagatcct cctcgtggct ccagcgcccg agtagggcct ggaacgccgc
       61 cagcaggaca acgaatgggc tggtcccttc acggacggcc agcgcccgga tcgcggcgct
      121 ctcctcccgc gagatgaata gggtctgtcg cgcgccgcgg taggtctgga ccggggggcg
      181 cggccggtcg gtgggcaggc gcatcaaggg ggggaggccg tcgagctgcc ggcgccagta
      241 ttcgagatgg ctctccagcc gcccgccctg gagctgctcg cgctgccaga cggcgaagtc
      301 ggcatactgc accggcagct ccggcaacgg cggtgcctgt ccagcgctga gcgcggcata
      361 cagggccgtc agctcacggg agaagacgct catcgaccag ccgtccgaga tgatgtggtg
      421 catggtgaag tgcaccagat ggtcggaggg cgagatccgc actacgagca ggcgcagcag
      481 cgtccctcgg gccaggtcga acggctggcg caggttctcc tgggcgagtt gccgggcgac
      541 cggatcgcgc aggggctccg gcaggccgtt caggtcgacg acgggcacgg ggatctcgag
      601 tcggggcacg acgacctgga cgggctcgcc gccgctttcc gccgcgaaat aggtgcgcag
      661 ggtctcgtgc cggccgacga tctcctgtgc tgcggcgatg atccgcggga tggacaacgg
      721 ccccctggcg aggacccctc ccgggatgtt gtacgccgag ctgccgggct cgatctggtg
      781 gaggatccac tcgcggagct gcgagaaaga gactgggaat cggccgggct ctcccggggt
      841 ccggggctgg cgtgggatgc gattggaccc cgccggcgcc tgcaccccgg agatcacggc
      901 ggccagctcg gccacggtgg aattttcgaa cagcgcccgt agctcgatct ccgcctggag
      961 ctcttcccgt accgccgcga gcacacgatg ggcgagcagc gagtggccgc ccagggcgaa
     1021 gaagttgtcg tggatgccga cccgctcgac gccgagaagg cgggaccaga tggcggcgag
     1081 ctcctgctcc atcgcgtcgc gcggcgccac gtactcggcc tccgaacgga gcgcgatgtc
     1141 cggagcgggc aggcgccgcc ggtcgagctt gccactgggc gaaagcggca tctctccgag
     1201 gaagacccag agggcgggaa ccatgggctc gggcaggcgg gccaggaggt gctcgcgcag
     1261 gttggccggc gtgagatccc ccacctcaag ccgcggcacg acgtagccgg tcaggcggac
     1321 ctccccccca tctgtgtgag ccaggaccgc ggcttcgcgg atgccgggat gcagcgtgag
     1381 cgccgcttcg acctcgccca gctcgatccg gaagccccgt accttgacct gatggtccat
     1441 ccgacccagg aagtcgaccg gcccgtccgg cagccggcgt gccaggtcac cggtgcggta
     1501 gagacgcgct ccaggctcac cgaaggggtc ggggacgaag cgttcggcgg tgaggcccgg
     1561 ccggtcatgg tagccacgtc cgacctggac accaccgata tacagctccc ccggggtacc
     1621 caccggcacc ggctggagga attcgtcgag caggtggatc cgggtgttgg ccaccggccg
     1681 gccgatgggc acgatggaga gctcgccgcc gttgcccccg atatcgcagg gctggaacgt
     1741 cacgtcgacc gccgcctcgg tggggccgta gaggttgtgc agctccacgc tccggcccaa
     1801 ccgctcgaaa aagcgctgcc gcaggtcggg cggcagggcc tcaccgctcg ctatgacgca
     1861 ccgtagggcg gcgcagcggc cgagcccccg ctcctccaga aagagccgca tcatggaggg
     1921 gacgaagtgg agcgtggtga tctcccgctc aaggacggtc tccaccagat aggcggcgtc
     1981 ctgatgccct ccggggcggg ccaccaccag gcacgcaccg gtgagcagcg gccagaagag
     2041 ctcccagacc gagacatcaa agctgaacgg cgtcttttgg agaatccggt cagcgggggt
     2101 gagcccgtac gtccgctgca tccagagcag ccggttgacg atccccgcat gggagttcat
     2161 cgcgcccttg ggcctgccgg tggagcccga agtgaagatg acgtaggcga gctggccggg
     2221 gccggcaccg acaaccggca cagccggcgc ggccggcccg accggaggct ccgggtccgg
     2281 cggcggtacg tcgagagcga tcaccctgaa cggaccggcg gtgccgcccg cggggagggg
     2341 cagggcggcg agcagccgct cctgggtgag cagcaccggc aggcgggcgt cctccagcat
     2401 catctccagg cgttccgccg ggtaggaggg atcgatgggc acgtaggccc ccccggcctc
     2461 gaggatcccc agaaggccca caagcatctc cagagaccgc tcgaccgcga tccccacccg
     2521 cgcttcgggg ccgacgccca gccgccgcag gcgtcccgcc agctctcccg cgcgccgcgc
     2581 cagctcggcg tacgagaggc tcgcctcctc gaaaactacg gccaccgcgt cgggcgtgcg
     2641 cgccgcctgc gccgcgatgg catggtggag caggaggtct cgcgggaagt ccacgcgggt
     2701 gtcgttccac tccaggagga gctggtgccg ctccgcctcg gtgagcagac cgatggtctc
     2761 gtaccggcca tcgggatcgg cggccatcgc cgcgagcacg cgctggaacc agccggacat
     2821 tgcccgcacc tgctcggtgt gcagggcctc accgtcgtac tccattgcca ccccgacctg
     2881 ctggccggag gagcggggtt ggagcgccac ctggagcggg tagttggtcc cttcgaggcg
     2941 cttcatcccg agcacctgga catcgccggt gcccagcagg tcgtcgacga catagaagtg
     3001 gatgtagttg aacatcacct cgaagagcgg acggccgccc cgcgcctgct ggaggagagc
     3061 gagggggtac cggcggtagg ggagctgctc ccactccgcc gtgaaggcct ggcgcgcgag
     3121 gtccttccag gtgcccccct ccaggcgcag acggaggggc agggtgttca ggaagaggcc
     3181 gcgcaggtgg tcgccgccgg gcacctcagg ccggccgttg gtgaccaggc cggtgactac
     3241 gtcggaacgg ccgctcgcca gggcgagaac ccgcaggtgc gcggcgagca gcacgctctt
     3301 gagcggcacg gcggcgatgc gggccagccg gtgaatcccg gtccccactt cgggagtggt
     3361 gacggcatcg agcgtgcggg tcctccgtcc gccggcacgg tgcggcacgg gccagcgggg
     3421 aatctcggtg atctctaccc ccgcgagcgc ctgctcccag aagcggcgcg cctcctccga
     3481 ggcgaccgtc tcgcgctcga tccgcacgaa gtcgcggtag gtgacggccg gggcgggctc
     3541 ctcacgtggc ggtgtgccgg cgagcagggc gaaatagagc tcgaagagct cggccagcgt
     3601 cgagtggaga ctccagccgt cgaagatcgc gtggttctcg gcgatggtga gctggaaagt
     3661 cgcgtcgctt cgccggtgaa cggcgatgcg gagctgcggc gcctggcgaa ggtcgtggag
     3721 ttggcgcttc tccgcctcga tgaaggtgtc gatcacggct tcctgcgcgt ctcgcgagag
     3781 atggcgcagg tcctcctcag cgaccggcag caccgcctcc cggtgcacga gctgcagcgg
     3841 ctcgctgaaa ccttcgaggt gaaacgaagt gcggagcacc gcgtggcgcg cgacgaccgt
     3901 gtcaacggct cgctggaaga cctccgtctc gaaacgcgcc tccaggagcc agctgtcgat
     3961 attgtggtag ggcgcgtcct cgcgtgagag gaccatgtgg aaaagcatcc ctgcctgaag
     4021 ggcggctagc ggataggcgt cctccacatc ctctggcagg agggcccggt ccgccgcgga
     4081 aaccaggctg aacggctcac tcttgaggac ggcggtggcc ccctccgtgg caggagccag
     4141 ctcggctgcc agcgcttcga cggtctgatg ctggaaaagc tgctgcaggt tgaagtcgag
     4201 cccccgctcg cgcgccagtg agatcacctg gatgctgcgg atcgaatccc cgcccaaggc
     4261 aaagaagttg tcgcgcacgc cgacccggtc gactcccagc acctgggccc agaccgcggc
     4321 cagtagctcc tcggtggggg tggacggcac cgcgaattcc tcgtcgaggt cgcgacgcgc
     4381 cggcccgggc gccggcaggg cgcggcgatc gagcttgcca ttggcggtga gcggcagctc
     4441 gtcgaggacc acccagacgg cggggaccat gtagtcagga agacgacgcc gcaggtcttc
     4501 ccggagggtg gcggtcgaga gagcttcgcc ggcgggaacg acgtaggcaa ccaggcgcgt
     4561 ctctccgccc ccctcgcttg gaaggacgac cgcctgccgc acctcgggcc gctcggcgac
     4621 ggcggcctcg atctccccca actcgattcg gaaaccgcgg atcttcacct gctggtcgat
     4681 ccgcccgagg tattcgaggt cgccgtccgg ccgccgccgg gccaggtcgc cgctgcggta
     4741 aaggcgcgcg ccaggctcgc cgccggaggg atcgggcacg aaacgctggg cggtcagctc
     4801 cgggcggccg agatagccgc gggccaggcc ggcgccgccg acgcagatct cccccggcac
     4861 gcccaccggc gccggtgaac ccgccaagtc cagaacgtgg agggacaggt ccgggatcgc
     4921 ttggccgatc aggctcctcc ctgccgccag gtcggcgcgg cgcatcggcc tgtaggtcac
     4981 gtgcacggtg gtctcggtga tgccgtacat gttgaccagc accggacgct cgtcgccatg
     5041 gcggttgaac cagggggcaa ggctcggaat gtccagggcc tcgccgccga agacgaccca
     5101 gcgcagtgcc aggcccgcgg cctcaccgcg ggcgccttcc gcttggacaa gctggcggaa
     5161 agccgagggg gtctggttga gcaccgtcac ccgctccctg accaggaggt ccaggaaatg
     5221 gtccggcgac cggctgacga ggtaggggac ggccacgagc cggccgccgt ggaggagggc
     5281 gccccagatc tcccataccg agaagtcgaa ggcgtaggag tggaacaggg tccagacgtc
     5341 gtccggacca aagccgaacc aggcatcggt ggccgcgaac aggcgcagga cgttggcgtg
     5401 ggtcaccacg acacccttgg ggcggccggt ggaccccgag gtgtagatca cgtaggccgc
     5461 ctgatccggc gccgcgtcgg ggagctcctc gccaatcgcc atctccccct ccagcgccac
     5521 gacctgaagg ccgttcccca aggcggcgag ccgctcctca aagccgggtc cggccacgag
     5581 cagagcggcg ccggagtccc gcgccatgaa ggccagccgc tcgtcgggat gggccgggtc
     5641 gagagggacg taggcgccgc cggccttgag gatgcccagc acgccgacca ccagcgccgg
     5701 gctccgttcg gccagcaggc agacccggga ctccggaccg acacccaagg cccgcagacg
     5761 gccggcaaga cggccggcgc gggcgtccag ctcggcgtag gtgatccgct cgccgtctcc
     5821 agtgaccgcc tcggcctgcg gcgcctgggc cgcctgttcc tcgaagcggc ggtggagcga
     5881 gcccgcgaca gggaacctgg cggtggcggc actccacgcc gtcgcctgcc gcctctcctc
     5941 ggcggagagg aggggcagct ccgacaggcg ccggtccgga tcgcgggcga tctcctccag
     6001 caaagtcccg aaatgcccgg ccaaacgctc gacggtagtg cggtcgaaga gatcggtgct
     6061 gtagtcgatg gagccggcga ggccgcccgg catctccgtc atggagagcg tcaggtcgaa
     6121 cttggaggtg cccgtgggcg ccggcagcgg cgccagtgac agcccccgga cctcgacggc
     6181 cggcgccggc gcgttctgca gggcgaaggt gacctggaag agcggcgtgt gggagacctg
     6241 ccgggagagg ccgagctcgt ccacaagcat ctcgaacggg atgtcttgat ggtcgtaggc
     6301 gccgagcgtc gcctcccgca cccggacgag caactcgcgg aacgtggggt ttcccgagag
     6361 gtccgcgcgt agggccaggg tattgacgaa gaagccgatc agcgcttcga cctcgtcccg
     6421 gttgcggttg gcgatgggag agcccacgac cacgtcatcg cgtccgctgt accgctggag
     6481 cacgatcgcg aaggcggcga ggagcaccat gaacagggtc gcgccctcgc gcctcgccag
     6541 ggcgtcgagc ccgtcgcgca cccccgaggg caggacgaag gagagggtcg aacccgccca
     6601 gccctgaacc gccggccgcg gccggtccgt cggcaggtcc agcaggtcgg gggcgccggc
     6661 caatcgctcg cgccaccagc cgagcagctc atccagggcc tcgccctgga ggcggcggtg
     6721 ctgccaggcg gcgaaatcag cgtactggat gggcagatcg gggagagggg atggccgccc
     6781 ggccgcgaag gcttcgtaga gcgtcacgac ctcgcgcacc aggatgccca tggaccagcc
     6841 gtcggagacg atgtggtgct ggaggaggac gagcacatgc tcctcggcgc cgagacggag
     6901 ggcggcggca cgcagcagcg gccccgcggc caagtcgaag gggcggcgcg cctcctcgtc
     6961 cacgagccgc ctggcctcgg cctgtcgggc ctcgccgggg agccgctcca gatcgatcac
     7021 cggcagcggg aagggaccgg ccggcgccaa tgcctggacc gcttgtccct cctccgtacg
     7081 gatcgtcgag cgcagcacct cgtgccggcg gacgacctcc gccagggcgc ggccgagggc
     7141 gtc
//
LOCUS       DASPJI010000402         6015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_710289, whole genome shotgun sequence.
ACCESSION   DASPJI010000402 DASPJI010000000
VERSION     DASPJI010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710289"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VEL74_10775"
     CDS             complement(<1..256)
                     /locus_tag="VEL74_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD regulator"
                     /protein_id="HYG63056.1"
                     /translation="METTPMGKAVLGLNAVIVAVTEEVPRILTVRTPGPTPETPEEDA
                     LPFGPLEPETDRTLDRALRRWVHEQTGLELGYVEQLYTFGD"
     gene            complement(320..1759)
                     /locus_tag="VEL74_10780"
     CDS             complement(320..1759)
                     /locus_tag="VEL74_10780"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HYG63057.1"
                     /translation="MVPIEQVDETRWRIPRHGKMRVDGLVFASAEMMEDIQKDESLQQ
                     VANVAHLPGILKASMAMPDIHWGYGFPIGGVAATDADEGVVSPGGVGYDINCGVRLLR
                     SSLHRDEIRPRLKELVNQLFRDVPTGMGATRHDLRLGKKDLQQVLERGASWAVEKGLG
                     TSRDLEVIEEGGTIHGADPSQVSDRAYERGGPQLGSLGSGNHFAEIQYVAEIYDEEAA
                     RAFGLTEGQVTVMIHSGSRGMGHQVCTDHLKVMLEAAARYGIELPDRQLCCAPIHSPE
                     AKRYFAAMAAAANFAFANRQVMCHSARQTFAKVLEVAPHQLGMETVYDVCHNIAKFET
                     FEMDGKKVRACVHRKGATRAYPAGHPQVPRPYREVGQPVMIPGDMGRYSYVLAGGDRA
                     YAETFGSTCHGAGRKLSRNEAKRVAKGRSILKELEEQGVLVKAAGMGTVAEEMPEAYK
                     DVADVVDVVHKAGLSRKVAQLRPMGVVKG"
     gene            1957..2292
                     /locus_tag="VEL74_10785"
     CDS             1957..2292
                     /locus_tag="VEL74_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63058.1"
                     /translation="MLIRTCRIALLTLCLGLTVQVLGAANDDLRQGPAARNAKSTSVA
                     GHFQFQPNDMEKVYEESLDDSFDDSFGGKINCRWTCANGNTGSATTDTVQQCYAACEG
                     ACGGRCGPA"
     gene            complement(2352..2774)
                     /locus_tag="VEL74_10790"
     CDS             complement(2352..2774)
                     /locus_tag="VEL74_10790"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HYG63059.1"
                     /translation="MGYRFLNHTADVAFDVEAPTRDELFAEALAAFTDTLTERETVEE
                     RVTRRFELRSSLLDLLLVDWLTELLYSFETEGLLFRRADVRVTEEDGAFLLTADARGE
                     AEDPERHQGKVLVKAVTYHGLEVVEEGDGWRARVVFDI"
     gene            complement(2756..3469)
                     /gene="lipB"
                     /locus_tag="VEL74_10795"
     CDS             complement(2756..3469)
                     /gene="lipB"
                     /locus_tag="VEL74_10795"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HYG63060.1"
                     /translation="MTAATPSRPLHWSFLGRVPYAEAVELQHSTREEVKRGGPDHLLL
                     LEHPHVYTLGRNADQGDVLAGEEWLRARGVEVAECDRGGQVTYHGPGQLVGYPIINLS
                     PDRRDIRRYVRDLQESLIRTLADHGVAAEPRDGQAFVGVWAGERKIASIGVHLSRWVT
                     THGFALNVSTDLSYFAGIVPCGLHQVEMTSIERLAGRAPALPEIAEVFAGHFARIFER
                     DLVSSPSPAAVPRVWATGS"
     gene            complement(3539..3850)
                     /locus_tag="VEL74_10800"
     CDS             complement(3539..3850)
                     /locus_tag="VEL74_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63061.1"
                     /translation="MKISARSLLIAALAVCAGLTMTVPASACYQECVEVEPFCERCQD
                     AGYLTGIACRNIGTCACKYYFPVCPDPLAAQPEPETQASLADLGISTEPEPAQCSATT
                     E"
     gene            complement(4382..4909)
                     /locus_tag="VEL74_10805"
     CDS             complement(4382..4909)
                     /locus_tag="VEL74_10805"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HYG63062.1"
                     /translation="MTGEELFRRPDLNPCELVNGRVVPLSPAGAIHGKVELRLGSKLL
                     TYADESGRGLAMSGEVGIWIRRDPDTVRGADLLFISRERYARRGTSGFLDVAPELVIE
                     ILSPTDRWSEVVEKIADYFAAGVNRVWVVDPTRRRVSSYGSSTEIRYFEIGGILTDEE
                     LLPGFGVQTSDLFRE"
     gene            complement(5048..>6015)
                     /locus_tag="VEL74_10810"
     CDS             complement(5048..>6015)
                     /locus_tag="VEL74_10810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HYG63063.1"
                     /translation="VNLEEVQGTGAQGRVTKEDIERYLEQRKGAPQAAPAAAPAPQAA
                     APAPAPAPAPAPAAAAAAPAGQRPQPAGFHVPAYGPGENVEIEPMSKMRQITAAHMVY
                     SKATSAHVTTVFHIDMSKVHRARLRAKDGFQKKEGTKLTYMPFIFKAVATALKAHKTV
                     NAAIDGTNIVYKKDINIGMAVDLPGRGLIVPVIKNADQLSLVGLAKTANDLADRARGK
                     KLKPDEAQGGTFTITNPGVFGSLFGTPVINQPQVAILGIGAIEKRPIVVEDADGNDAI
                     VIKTMSYFGISYDHRLVDGADADRFMIDVKKVLEQDTWTELEAYV"
BASE COUNT          962 a         2005 c         2055 g          993 t
ORIGIN      
        1 ggtcgccgaa ggtgtagagc tgctcgacgt agccgagctc caggccggtc tgctcatgca
       61 cccagcggcg cagggcacgg tcgagcgtcc ggtcggtctc cggctccagc ggaccgaagg
      121 ggagggcatc ctcttccgga gtttcggggg tggggcccgg cgtgcgcacg gtcagaatgc
      181 gcggcacctc ctcggtgacc gccacgatga ccgcgttgag accgaggacc gccttgccca
      241 tgggagtcgt ctccattccc ctcatcttct ctcgtgaagg gagcggaaga aagggggaca
      301 agcggccccc caggaaccgc tagcccttga ctacacccat ggggcggagc tgcgccacct
      361 tgcgggagag gccggcctta tggaccacgt ccaccacgtc cgccacgtcc ttgtacgcct
      421 cgggcatctc ctcggcgacg gtgcccatgc cggcggcctt caccagcacc ccctgctcct
      481 ccagctcctt gaggatggag cggcccttgg ccaccctctt cgcctcgttg cggctcagct
      541 tgcggccggc gccgtggcag gtggagccga aggtctcggc gtaggcgcgg tcgccgccgg
      601 ccagcacgta ggagtagcgg cccatgtcgc ccgggatcat caccggctgc cccacctcgc
      661 ggtagggccg gggcacctgg gggtggcccg ccggataggc ccgcgtcgcc cccttgcggt
      721 ggacgcaggc gcggaccttc ttgccgtcca tctcgaacgt ctcgaacttg gcgatgttgt
      781 ggcagacgtc gtagacggtc tccatgccca actggtgcgg ggccacctcc agcaccttgg
      841 cgaaggtctg ccgggcactg tggcacatca cctggcggtt ggcgaaggcg aagttggcgg
      901 cggcggccat ggccgcgaag tagcgcttcg cctcggggga atggatcggc gcgcagcaga
      961 gctggcggtc gggcagctcg atgccgtagc gcgccgcggc ctccagcatc accttgaggt
     1021 ggtcggtgca gacctgatgc cccatgccgc gggaaccgct gtggatcatc accgtgacct
     1081 gcccctcggt gaggccgaac gcccgggccg cctcctcatc gtagatctcg gcgacgtact
     1141 ggatctcggc gaagtggttg ccggagccca gggagccgag ttgcgggccg ccccgctcgt
     1201 aggcgcggtc gctcacctgc gacgggtccg ccccgtggat cgtccccccc tcctcgatga
     1261 cctcgaggtc gcgggaggtg ccgagcccct tctcgacggc ccagctggcg ccgcgctcca
     1321 ggacctgctg gagatccttc ttgcccaggc gcaggtcgtg gcgcgtcgcc cccatgccgg
     1381 tgggcacgtc gcggaacagc tggttcacca gctccttgag gcgcggccgg atctcgtcgc
     1441 gatggaggga cgagcgcagc agccggacgc cgcagttgat gtcgtagccg acgccccccg
     1501 gggagaccac cccctcgtcg gcgtccgtcg ccgccacgcc accgatgggg aagccatacc
     1561 cccaatggat gtccggcatg gccatggagg ccttgaggat tccgggcaga tgcgcgacgt
     1621 tcgccacctg ctggaggctc tcgtccttct ggatgtcctc catcatctcc gcgctggcga
     1681 agaccagccc gtccacccgc atcttgccgt gccgcggaat ccgccagcgt gtctcgtcga
     1741 cctgttcgat cggaaccatg ttctcgcctc ctcccgctcc cccgccgcct gcacgacccg
     1801 taccgtgggg accggagcca gggcgaatac tacatgctga tccgaggagg aggatcgcct
     1861 cggctcccga aaaagctata tagacatttg gctctgcatc gcctaaggta cagaaccaga
     1921 cgcgcatcac gccgtcttca ttctttggag gagagcatgc tgatcaggac atgcaggatt
     1981 gctctactga cgttgtgtct gggactcacg gtgcaagtac ttggagcggc caacgacgac
     2041 ctgcgccagg gtcccgcggc acgtaacgcc aagagcactt ctgtcgctgg acacttccag
     2101 ttccaaccca acgatatgga aaaggtctac gaagagtctc tcgacgactc tttcgatgac
     2161 tcgttcggcg gcaagatcaa ttgccgttgg acctgcgcca atggcaacac cggttcagct
     2221 acgaccgaca ctgttcagca gtgttacgct gcctgtgagg gtgcctgcgg tgggcgttgc
     2281 ggcccggcgt gaaatctgag gtaaaaatgt agtcttctcg ctggtggagg gccccttggg
     2341 ctctccacca gtcagatgtc gaacactacc cgcgcccgcc agccgtcccc ctcctccacc
     2401 acctccagcc cgtgataggt caccgccttg accaggacct tgccctggtg acgctcgggg
     2461 tcctccgcct cgccccgggc gtcggcggtg agcaggaagg cgccgtcttc ctcggtcacc
     2521 cggacgtcgg cgcgccggaa gaggagcccc tcggtctcga aggagtagag cagctcggtc
     2581 agccagtcca ccagcagcag gtcgagcagg gacgaccgca gctcgaaccg gcgcgtcacc
     2641 cgctcctcca ccgtctcgcg ctcggtgagc gtgtcggtga aggcggcgag ggcttcggcg
     2701 aagagctcgt cgcgggttgg cgcctccacg tcgaaggcga cgtcggcggt gtggttcagg
     2761 aaccggtagc ccataccctg gggacagccg ccggggaggg agaggagacc aagtcgcgct
     2821 cgaagatccg cgcgaagtgg ccggcgaaga cctcggcgat ctcggggagc gccggagccc
     2881 tgcccgccag cctctcgatg gaggtcatct ccacctggtg caggccgcag gggacgatgc
     2941 cggcgaagta cgagaggtcc gtggagacgt tgagggcaaa gccgtgggtg gtcacccagc
     3001 gggagaggtg gacgccgatg gaggcgatct tcctctcccc ggcccagacg cccacgaagg
     3061 cctgcccgtc gcgaggctcc gcggcgacgc catggtcggc gagcgtccgg atcagcgact
     3121 cctggaggtc gcggacgtag cggcggatgt cccgccggtc ggggctcagg ttgatgatgg
     3181 gatagccgac gagctggccc ggcccgtggt aggtgacctg gccgccgcgg tcgcactccg
     3241 ccacctcgac gccgcgggcc cgcagccact cctcgcccgc cagcacgtcg ccctggtcgg
     3301 cgttgcgtcc cagggtgtag acgtgcgggt gctcgagcag gagcaggtga tccggcccgc
     3361 cccgcttgac ctcctcgcgg gtcgagtgct ggagctccac cgcctcggcg tacgggacgc
     3421 ggccgaggaa gctccagtgc agggggcggg agggggtggc agccgtcatg ccggcgattc
     3481 tagccggatt cggggagcgg cggggcggcg gccggagcac cgccccgcac ttgagggact
     3541 actccgtcgt ggcggagcac tgagccggct cgggctcggt ggagatcccc agatccgcga
     3601 gggatgcctg ggtctccggc tccggctgcg cggccaacgg atcagggcag acgggaaagt
     3661 agtacttgca cgcgcaggtg ccgatgttcc tgcaggcgat gccggtcaag tagccggcgt
     3721 cctggcaccg ctcacagaag ggctcgacct ccacgcactc ctgatagcag gcggacgcgg
     3781 ggaccgtcat ggtgaggccg gcgcagaccg ccagcgcagc aatcagcaac gacctggcag
     3841 agatcttcat aggctctcct ctctcgcggg atgcgacctg tcgcacctac tcaagaaggt
     3901 gcggcgcggc ggagagaagg gacagggggg cagccccccg gaggcgcgct ccggggggcc
     3961 accgcctaga cgcagtaggg atacggggga ttgccgcagg ggcagtaggc gcactggccc
     4021 gggagacagc cgatactcga ccagtagccg ttggcgcact gccgggcgca cgaagaggaa
     4081 caggaggttc ctccccccgt gcccgggtct ttcgtccacg cgctcagcgg cagactcgac
     4141 agaacgagaa cgagccccaa cagaagcagc ttgaccttta ccttgatgct caatgccttc
     4201 tcctttcgac gaagaggacc ctggcccatc gcggacctcc ggtacgtggg cgcacctgga
     4261 aagctcctcg atgacagagg cgcgacttca gggtgttttc cgtcaggcag ctgccggctg
     4321 ccggcacaga cacgacgggc ccggatcgct ccgggcccgt cgggggaaca gcggtgtgag
     4381 gctactcgcg gaacaggtcg gaggtctgca cgccaaaacc gggcagcagc tcttcgtcgg
     4441 tcagaattcc cccgatctcg aagtaccgga tctcggtcga ggagccatag gaggagaccc
     4501 ggcgacgtgt gggatcgaca acccatactc gattcacacc ggctgcgaaa tagtcggcga
     4561 tcttctctac cacttcgctc cagcgatcgg tgggtgaaag gatctcgatc accagctcgg
     4621 gggcgacgtc gagaaagccc gaggtgccgc ggcgggcata gcgctcacgc gagatgaaca
     4681 ggaggtcggc gccgcggacg gtgtctggat cccggcggat ccagatcccc acctcgcctg
     4741 acatcgccaa gcccctcccg ctctcgtctg cgtaggtgag cagcttagag ccgagtctga
     4801 gctccacctt gccatggata gcccctgccg ggctcaaagg cacgaccctc ccgttcacga
     4861 gctcgcaggg attgaggtca ggacgccgga aaagctcctc gccggtcatc aacctctctt
     4921 cggatctctg gagcagagtc acgttctacc tcctctccta ttctatcaaa catcagtcgc
     4981 gccaagacac gacgggcccg gatcgctccg ggcccgtcgg ggaacagcgg tgtgaagcag
     5041 gagaggccta gacgtaggct tccagctcgg tccaggtgtc ctgctcgagc accttcttga
     5101 cgtcgatcat gaagcggtcg gcgtcggcgc cgtccaccag ccggtggtcg taggagatgc
     5161 cgaagtagga catggtcttg atgacgatgg cgtcgttgcc gtcggcgtcc tccaccacga
     5221 tcggccgctt ctcgatggcg ccgatgccca ggatcgccac ctgcggctgg ttgatcaccg
     5281 gcgtgccgaa gaggctgccg aagacgccgg ggttggtgat ggtgaaggtg ccgccctggg
     5341 cctcgtcggg cttgagcttc ttgccgcgcg cccggtcggc caggtcgttg gcggtcttcg
     5401 ccaaacccac caggctcaac tggtcggcgt tcttgatgac cggcacgatc aggccacggc
     5461 ccggcaggtc caccgccatg ccgatgttga tgtccttctt gtagacgatg ttggtgccgt
     5521 cgatggcggc gttgaccgtc ttgtgggcct tgagcgcggt ggccacggcc ttgaagatga
     5581 agggcatgta ggtgagcttg gtgccctcct tcttctggaa gccgtccttg gcgcggagcc
     5641 gggcacggtg caccttcgac atgtcgatgt ggaagaccgt cgtgacgtgg gccgacgtgg
     5701 ccttggaata gaccatgtgg gcggccgtga tctgccgcat cttggacatc ggctcgatct
     5761 cgacgttctc ccccgggccg taggcgggca cgtggaagcc ggcgggctgc ggacgctgtc
     5821 cggccggagc cgccgccgcg gcagccgggg ctggcgcggg ggccggagcg ggagcgggcg
     5881 ccgcggcctg aggagcggga gccgccgccg gagccgcctg gggagcgccc ttgcgctgct
     5941 ccaggtaacg ctcgatgtcc tccttggtga cccggccctg ggcgccggtg ccctgcacct
     6001 cctcgaggtt caccc
//
LOCUS       DASPJI010000403         3132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_196733, whole genome shotgun sequence.
ACCESSION   DASPJI010000403 DASPJI010000000
VERSION     DASPJI010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196733"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="VEL74_10815"
     CDS             <1..1286
                     /locus_tag="VEL74_10815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYG63064.1"
                     /translation="PDGRRIAFVASPGGEPVRSYNQPDLFVVDTAAGSAPRNLTADYD
                     FDINARLTGDQEPPRGERPDQPLWSADGRSLLVRAAERGRAGLVRVDAASGRVEPFLP
                     GDHEVASYTASRDGSRLALVISTPLVLNDLHLLETATGRMRPLERPNEALFSELALSA
                     PEEITYTSFDGRQIQAWLLKPPGFDPAKKYPVILEIHGGPHAAYGHVFDHEFHWLAAR
                     GYLVLFPNPRGSSSYGQEFGNVIQFDFPGNDSKDLVAGVDELIRRGMVDPQRQGVTGG
                     SGGGVLTNWIITQTDRFAAAVSQRSIADWTAFWYGADFTLFEPFWFRGAPWEEPEDFA
                     ARSPITHVKNVKTPLMLMEGEADWRTPSWAGGELMFRALRYLGKPTVMIRFPGEDHDL
                     SRTGGPWHRVERLQHMVAWFDKYLLGRGGEQYDLN"
     gene            1331..1903
                     /locus_tag="VEL74_10820"
     CDS             1331..1903
                     /locus_tag="VEL74_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63065.1"
                     /translation="MTEPGPEGNPNAMSQKVGDEALRAKTGRIWEEWFAVLDDVGAVE
                     KGHTEIARYLHEEMGVDGWWAQMVTTGYEQARGKRVKHQKADGYEVSASKTLPVPLDT
                     LFQAWQDDTVRGRWLPEPGVVVRKANPGKTMRLGWPDKTLVAVYFSAKGEDKSAVQVQ
                     HGKLPDSGAAARMKSWWGETLERLRAELQA"
     gene            complement(1907..3100)
                     /locus_tag="VEL74_10825"
     CDS             complement(1907..3100)
                     /locus_tag="VEL74_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63066.1"
                     /translation="MPFFTDFIYRLREYGVPVSLTDTLDFYKGLEKGLAPDLESLFVF
                     ARLCFVRRVEHMDPYERAFAFHFHGIDLPRVAEGDPELLYTPQFREWLQEMIRKGELP
                     PHALWTMEPEELMKRFWDTVRQQMEKHQGGSRWVGTGGSSPFGHSGNSSGGVRVHGAS
                     MGRSALKVIGERRYVEYSDTTTLQGANLGQALASLKTMVPSGPRDQLDLPATIDQSAR
                     NGGEIELAFRRDLLDRIEVLLLIDNGGSSMLAHVPLTQLLFAKARDRFKRLTTCFFHN
                     TIYGSVFRDSRHSSVIQTAELLRRPSGTRLLLVGDASMAPAELIYRRGSIGWGIDDEE
                     PSLVWLQRLRDRFRHSVWLNPIPKESWPDTYGRATIQKIGEVFRMEDLTLGGIKRAVE
                     YLSRP"
BASE COUNT          498 a         1127 c         1065 g          442 t
ORIGIN      
        1 cgcccgacgg gcgccggatc gccttcgtcg cctcccccgg cggggagccg gtgcgctcct
       61 acaaccagcc cgacctcttc gtcgtcgaca ccgccgccgg gagcgcgccg cgcaacctca
      121 ccgcggacta cgacttcgac atcaacgccc gcctgaccgg cgaccaggag ccgccgcgcg
      181 gcgagcgccc cgaccagccg ctgtggagcg ccgacggccg ctccctcctg gtgcgcgccg
      241 ccgagcgcgg ccgggccggc ctggtgcggg tcgacgccgc ctcggggcgg gtcgagccct
      301 tcctcccggg cgaccacgag gtcgcctcct acaccgcctc gcgcgacggc tcgcgccttg
      361 ccctggtgat ctcgacgccg ctggtgctca acgacctcca cctgctggag acggccaccg
      421 ggcggatgcg ccctctggag cggcccaatg aagccctgtt ctcggagctc gccctctccg
      481 ctcccgagga aatcacctac accagcttcg acggccggca gatccaggcc tggctgctga
      541 agccgccggg cttcgacccg gcgaagaagt acccggtcat cctcgagatc cacggcggcc
      601 cgcacgccgc ctacggccac gtcttcgatc acgaattcca ctggctggcg gcccgaggct
      661 acctcgtcct ctttcccaat ccccggggga gctcgtccta cggccaggag ttcggcaacg
      721 tcatccagtt cgatttcccc ggcaacgact ccaaggatct ggtggccggc gtcgacgagc
      781 tgatccgccg cggtatggtg gacccgcaac gccagggggt gaccggcggc agcggcggcg
      841 gcgtgctcac caactggatc atcacccaga ccgaccgctt cgccgccgcg gtctcgcaac
      901 gctccatcgc cgactggacg gcgttctggt acggcgccga cttcaccctc ttcgagccct
      961 tctggttccg cggcgccccc tgggaggagc cggaggactt cgccgcccgc tcgccgatca
     1021 cccatgtgaa gaacgtgaag accccgctga tgctgatgga gggggaggcc gactggcgca
     1081 ctccgtcctg ggcgggcggc gagctgatgt tccgcgccct gcgctatctc ggcaagccca
     1141 ccgtcatgat ccgcttcccc ggcgaggacc acgacctgtc gcgtaccggc ggcccctggc
     1201 accgcgtcga gcgcctccag cacatggtcg cctggttcga caagtacctc ctaggcaggg
     1261 ggggcgagca gtacgacctg aactgagggg gctctggtac aatggcggcg taaccgagag
     1321 gagaagcgcc atgacggaac ccggccccga aggcaacccg aacgcgatgt cgcagaaggt
     1381 gggcgacgag gccctgcgcg ccaagaccgg caggatctgg gaggagtggt tcgccgtcct
     1441 cgacgacgtg ggcgccgtgg agaaggggca cacggagatc gcccgctacc tccatgagga
     1501 gatgggggtc gacggctggt gggcccagat ggtgaccacc ggctacgagc aggcgcgcgg
     1561 caagcgggtg aagcaccaga aggcggacgg ctacgaggtc agcgccagca agacgctgcc
     1621 cgtccccctc gacaccctgt tccaggcctg gcaggacgac accgtgcgcg gccgctggct
     1681 gcccgagccc ggcgtcgtgg tccgcaaggc gaaccccggc aagaccatgc gcctcggctg
     1741 gcccgacaag accctggtcg ccgtctactt ctccgccaag ggcgaggaca agagcgcggt
     1801 gcaggtccag cacggcaagc tccccgactc cggcgccgcc gcccgcatga agtcctggtg
     1861 gggcgagacg ctggagcgcc tgcgggcgga gctgcaggcc tgaggtctag ggccggctca
     1921 gatactcgac agcccgcttg atgccgccga gcgtcagatc ctccatgcgg aagacctcgc
     1981 cgatcttctg gatggtggcg cgaccgtagg tgtcgggcca gctctccttg gggatcggat
     2041 tcagccagac ggagtggcgg aagcggtcgc gcaggcgctg cagccagacg aggctcggct
     2101 cctcgtcgtc gatcccccaa ccgatggagc cccggcgata gatcagctcc gccggcgcca
     2161 tcgaggcgtc gccgaccagg agcagccgcg tcccggacgg gcgccgcagc agctccgcgg
     2221 tctggatcac cgagctgtgc cgggagtccc ggaagaccga gccgtagatg gtgttgtgga
     2281 agaaacaggt ggtgagccgc ttgaagcggt cccgcgcctt ggcgaacagg agctgggtga
     2341 gcggcacgtg ggcgagcatc gacgagccgc cgttgtcgat cagcagcagc acctcgatgc
     2401 ggtccagcag gtcgcgccgg aaggcgagct cgatctcgcc gccgttccgg gcgctctggt
     2461 cgatggtcgc cggcaggtcg agctggtccc gcggtcccga ggggaccatg gtcttgagcg
     2521 aggccagcgc ctgccccagg ttggcaccct ggagggtggt ggtgtccgag tactcgacat
     2581 agcgccgctc gccgatcacc ttgagcgccg agcggcccat ggacgcgccg tggacacgca
     2641 cgccgcccga ggagttgccg gagtggccga acggcgagct ccccccggtc cccacccagc
     2701 ggctgccgcc ctggtgcttc tccatctgct ggcgcaccgt gtcccagaag cgcttcatca
     2761 gctcctcggg ctccatcgtc cacagcgcgt gcggcggcag ctcgcccttc cggatcatct
     2821 cctgcagcca ctcccggaac tgcggcgtgt agagcagctc cgggtccccc tccgccaccc
     2881 gcggcaggtc gatgccatgg aaatggaagg caaaggctcg ctcgtaaggg tccatgtgct
     2941 ccacccggcg gacgaagcag aggcgggcga agacgaacag cgactcgagg tcgggggcca
     3001 accccttctc gagccccttg tagaagtcga gggtgtcggt gaggctcacc ggcaccccgt
     3061 actcccgcag gcgatagatg aaatcggtga agaacggcac cgccgggcct ccccgtcagt
     3121 tcacccgcgc gc
//
LOCUS       DASPJI010000404         8426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_320117, whole genome shotgun sequence.
ACCESSION   DASPJI010000404 DASPJI010000000
VERSION     DASPJI010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320117"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1387
                     /locus_tag="VEL74_10830"
     CDS             242..1387
                     /locus_tag="VEL74_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HYG63067.1"
                     /translation="MRIELSDEHREVRDQVRRFAEEVVKPRAKEIDESGEFPRSFFDQ
                     AGELGLAGVAVPEEYGGAGMDNIAYCLVIEEISRVCATSGVILSVNNSLVCDPIMKFG
                     NDEQKREFLTPLAAGQKLGCFALTEPGAGSDAASLRATARRDGDDYVLNGNKVFITNG
                     THADVAIVFATVDPAKKHKGITAFLVSTDTPGYHHGTHEFKLGVNASGTTELAFNDMR
                     VPAKNRLGEEGDGFKIAMSTLDGGRIGISAQAVGIAQGAFEEATAYSQQREQFGQAIS
                     NFQAIQFYLADMATEIDAARLLTWKAAWTKDNGKRYTLEAAQAKLFSAEMAQRVTNKA
                     LQIHGGYGYTKEYNVERYFRDARITEIYEGTSEVQKMVIADWVLNKA"
     gene            1466..2605
                     /locus_tag="VEL74_10835"
     CDS             1466..2605
                     /locus_tag="VEL74_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYG63068.1"
                     /translation="MNFALSEDQEMVREQVRRFAEERIQPGVAERDREHRFPEEIIRE
                     IGELGLLGMMVAEEYGGAGMDTLSYLLAVEEIARVCPATAVTMSVTNSVCCWPIHRFG
                     SEELKRRVLPPLASGEAIGGFGLTEPGSGSDAGSMRTTARRDGDFWVLNGEKAWITNA
                     GFARYYVVVARTNPEAGKRGLSAIVVPADAPGFSVGQPEEKLGLKASRTAAIHFSDCR
                     VPVANLLGEEGQGLKIALATLDHSRLGIAAQSVGIHQRALELAVQYARDRVQFGVPIA
                     QHQAIQFKIAQIATELAAARALTYAGAAAEHSRDAGRLAAQAKVYASEAANRACQDSL
                     QIHGGNGFHEDYEIARLYRDVRVTTIYEGTSEMQRLVIARHLLQK"
     gene            2654..3955
                     /gene="rmuC"
                     /locus_tag="VEL74_10840"
     CDS             2654..3955
                     /gene="rmuC"
                     /locus_tag="VEL74_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HYG63069.1"
                     /translation="MDLSPEVFLLTLVAAALGAAIGWLLARQRAVSERLALETRVARL
                     ETALEKERQAAAEKLAVLEEARQTLSDSFQALSADALRNNNASFLHLARATLEKFQET
                     ARGDLERRQQAIGELVRPLRDSLQKVDLKLQEIETARAVAYNSLTEHLKNLASAHTNL
                     ERETSKLVGALRAPSARGRWGEIQLQRVVEMAGMVAYCDFATQATVETEEGRQRPDLV
                     VRLPNQKQIVIDAKAPLSAYLDALEVHDDEGRRAKLREHARQIRTHLAKLSQKTYWAQ
                     FEPAPEFVVLFLPGEIFFSAALEQDPSLIELGVEQRVILATPTTLIALLRAVAYGWRQ
                     AQVEESARTISELGRALHDRLRTFVVHLGTMRRGLDQAVDAYNRAVGSLEGRVLPAAR
                     RFKDLGAAGGDEIETLESVDKVTRSIESVEAVDAADLSQSL"
     gene            4058..4459
                     /locus_tag="VEL74_10845"
     CDS             4058..4459
                     /locus_tag="VEL74_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63070.1"
                     /translation="MSSSDSKAQSFVRRRALILGVAVILGTLALDVQAEPIPDGALAP
                     HRVGWFDNGGTMTCAETCKAKAPGSSAEHEPSPIAPTKRVFVCKVAGKPNGDLRTWLY
                     GNQFDDRPACYTTGLDLKGSYSKKFSCLCVR"
     gene            complement(4525..4803)
                     /locus_tag="VEL74_10850"
                     /pseudo
     CDS             complement(4525..4803)
                     /locus_tag="VEL74_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            5033..6055
                     /locus_tag="VEL74_10855"
     CDS             5033..6055
                     /locus_tag="VEL74_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63071.1"
                     /translation="MATLREQLTKILPSVLPDRAEDAINGTDLLATVRGKLDGDYSEA
                     SVRTHFSVMSADPTSPIAKVDQGHGYYRRSLGTTAAAIPIDEGGAKSVTEEAAEEFLG
                     QRASQREEKFRSIYMRYAEITNQFPMHVEHTKGARQRAGVNKWKFPDVVVLRWEVGEV
                     SDAGFRLAREVLEVKKSLGEQPFKLISSELKVELAIGSFREAFFQCVSNSKWAHSAQL
                     AVAAKINDESLAEELRRLGTSYDVSVVSYGLEPEYLDSLPPADKILKMQTGDFEAIAA
                     NVAVTRVATGRDRETLDWEHLRDLRAQSEEFSNLFRWTAYCLEKKSPYRYEDWEKIAR
                     LESRYT"
     gene            complement(6101..6514)
                     /locus_tag="VEL74_10860"
     CDS             complement(6101..6514)
                     /locus_tag="VEL74_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYG63072.1"
                     /translation="MKLLLDTHFLIWLTMGSARLDEFPWLDRYHPWGISPVSLLEIQF
                     LAEVGRIGIRNPGFTETVTGDQRFLLDEAPLQPLVRHAIELTWTRDPFDRLLAAHSSV
                     RRIPLCTLDSVIRKHHRLLPNELAIDEEAPTRPTS"
     gene            complement(6511..6771)
                     /locus_tag="VEL74_10865"
     CDS             complement(6511..6771)
                     /locus_tag="VEL74_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63073.1"
                     /translation="MKRVTATEARKNWFRYLDEAAEGEVLLIERGGTRLVLHREAREE
                     EAPRHVPSYEGLIWAPHAEDADRWGWEWKEPGEALSPVDRDR"
     gene            complement(6850..8004)
                     /locus_tag="VEL74_10870"
     CDS             complement(6850..8004)
                     /locus_tag="VEL74_10870"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYG63074.1"
                     /translation="MIDNRTSYPAKLMSLALTFVVTGLLACSPASEGEPADAQAQGQA
                     QTQAQPGTAAPAPGATVAPQGQPLPGQVAAPGSAPAAAAPPMPADKIPAVVARINGQE
                     IRKDALLAEAERMQQQFAARLGQQTPATQEFYQKILDGMVAQQLLLAEAQAQGITVTE
                     AEVNAQIEPLRKRMGDEKFKQALTTEKMTEDKFKQEVRKNAVVEKLLASKVVSTVKVT
                     DQDIQAYYDQNKDEFKRPERARLRHILVRVEQNATAEAKQAARQKAEGLLARVKGGED
                     FAKVAQEASDDPGSKPRGGDLGWVAPGQTVEPFEKAAFALKPNEISPVVESRFGFHII
                     QLQEKQAADTAPLSEVKERLTTFLRTKQTGEAIEAHVNQLRSKAKIETFI"
     gene            complement(8116..>8426)
                     /locus_tag="VEL74_10875"
     CDS             complement(8116..>8426)
                     /locus_tag="VEL74_10875"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HYG63075.1"
                     /translation="AVLRLLAIGLLFSALGSVSAAVLQALGRPDLTARLRLAELLVFL
                     PAGALLIRQWGAEGAALGALLRAVLDASALHLIAGRQLRNGSPSSLGVESNKVPVRSG
                     "
BASE COUNT         1443 a         2752 c         2832 g         1399 t
ORIGIN      
        1 aggccccccc ggggttgccc atcaggttcc agcccgtggg caggacactg cggatctccg
       61 tctcggtgta gccgatcgct tcgttcgtgg tcttcatgac gggggcattc tgcaacaact
      121 ggcgccccgc agggaagccg cccttccttg accccccgca gggcggttgc tacgatccgg
      181 cgggcggtcc cgcaggtccc gcccccatcc agagagaaag cccagaggcc agaggagaaa
      241 catgcgcatc gagctcagcg acgagcacag ggaggtccgc gaccaggtcc ggcgcttcgc
      301 cgaggaggtg gtcaaaccgc gggccaagga gatcgacgag agcggggagt tcccgcgctc
      361 gttcttcgac caggcgggcg agctcggcct cgccggggtg gccgtccccg aggagtacgg
      421 cggcgccggc atggacaaca tcgcctattg cctggtcatt gaggagatca gccgggtctg
      481 cgccacctcg ggcgtcatcc tgtcggtgaa caactcgctc gtctgcgatc cgatcatgaa
      541 gttcggcaac gacgagcaga agcgggaatt cctgaccccg ctcgccgccg gccagaagct
      601 cggctgcttc gccttgaccg aaccgggcgc cggcagcgac gccgccagcc tccgcgccac
      661 cgcccgccgc gacggcgacg actacgtgct caacggcaac aaggtgttca tcaccaacgg
      721 cacccacgcc gacgtcgcca tcgtcttcgc caccgtcgac ccggcgaaga agcacaaggg
      781 gatcaccgcc ttcctggtgt ccaccgacac cccgggctac caccacggca cccacgagtt
      841 caagctcggg gtcaacgcct cgggcaccac cgagctggcc ttcaacgaca tgcgggtgcc
      901 ggcgaagaac cgcctcggcg aggaggggga cggcttcaag atcgccatgt ccaccctgga
      961 cggcggtcgc atcggcatct ccgcccaggc cgtcggcatc gcccagggtg ccttcgagga
     1021 ggccacggcc tactcgcagc agcgcgagca gttcggccag gcgatctcca acttccaggc
     1081 catccagttc tacctggcgg acatggcgac ggagatcgac gccgcccgcc tcctcacctg
     1141 gaaggcggcc tggaccaagg acaacggcaa gcgctacacc ctcgaggcgg cgcaggccaa
     1201 gctgttctcc gccgagatgg cgcagcgggt gaccaacaag gcgctccaga tccacggcgg
     1261 ctacggctat accaaggagt acaacgtcga gcgctacttc cgtgacgcgc gcatcaccga
     1321 gatctacgag ggcaccagcg aggtccagaa gatggtgatc gccgactggg tgctcaacaa
     1381 ggcctgacgt ccggaactgg agagttttgt catgggcgcc accctcgccg aacccgccac
     1441 ccccgccggc accacctccg ccggtatcaa cttcgccctg agcgaggacc aggagatggt
     1501 ccgcgagcag gtgcggcgct tcgccgagga gcgcatccag cccggcgtgg ccgagcgcga
     1561 ccgcgagcac cgctttcccg aggagatcat ccgcgagatc ggcgagctcg gcctcctcgg
     1621 catgatggtg gcggaggagt acggcggtgc cggcatggac acgctgagct atctcctggc
     1681 ggtggaggag attgcgcggg tctgcccggc gacggcggtg accatgagcg tcaccaactc
     1741 ggtctgctgc tggccgatcc accgcttcgg cagcgaggag ctgaagcgcc gcgtgctgcc
     1801 ccccctggcc tcgggcgagg cgatcggcgg cttcggcctc accgagccgg gctcggggag
     1861 cgatgccggc agcatgcgca ccacggcgcg ccgcgacggt gacttctggg tgctcaacgg
     1921 cgagaaggcc tggatcacca acgccggttt cgcccgctat tacgtcgtgg tggcccgcac
     1981 caaccccgag gcgggcaagc gcggcctgtc ggcgatcgtg gtgccggcgg acgctcccgg
     2041 gttctccgtc ggccagcccg aggagaagct ggggcttaag gcgtcgcgca cggcggccat
     2101 ccacttctcc gactgccggg tgccggtggc gaacctgctg ggtgaggagg ggcaggggct
     2161 caagatcgcc ctcgccaccc tcgatcattc acgtctgggc atcgcggccc agtcggtggg
     2221 catccaccag cgggcgctgg agctggcggt gcagtacgcc cgcgaccggg tgcagttcgg
     2281 ggtgcccatc gcccagcatc aggccatcca gttcaagatc gcccagatcg ccaccgagct
     2341 ggccgccgct cgcgccctca cctacgcggg agccgccgcc gagcactccc gtgacgccgg
     2401 gcgtctcgcc gcccaggcca aggtctacgc ctccgaagcc gccaaccgcg cctgccagga
     2461 cagcttacag atccacggcg gcaacggctt ccacgaggac tacgagattg cccgtctcta
     2521 tcgcgacgtg cgcgtcacca ccatctacga aggcaccagc gagatgcagc ggctggtgat
     2581 cgcgcggcac ctgcttcaga aataagcctt cgggcgtagg accggcggaa tccagggcgt
     2641 atactggagt ccgatggatc tatctcccga ggtttttctc ctgaccctcg tcgccgctgc
     2701 cctgggtgcg gcgatcgggt ggctcctcgc tcggcaacgg gccgtctcgg agcgcctcgc
     2761 cctcgagacc cgggtggcgc ggctcgagac cgctctcgag aaggagcgcc aggcagcggc
     2821 ggagaagctg gcggtgctcg aagaggcccg ccagacgctg tccgactcct tccaggccct
     2881 ctccgccgac gcactccgca acaacaacgc ctccttcctc cacctcgccc gcgcgactct
     2941 ggagaagttc caggagacgg cgcggggtga cctggagcgg cgccagcagg cgatcggcga
     3001 gctcgtccgg ccgctgcgcg actccctcca gaaggtggac ctgaagctcc aggagatcga
     3061 gaccgcccgc gccgtcgcct acaacagcct cacggagcat ttgaagaacc tggcctcggc
     3121 gcataccaac ctggagcgcg agacctcgaa gctggtgggg gcgctacggg caccgtctgc
     3181 ccgcggccgg tggggggaga tccagctcca gcgggtggtg gagatggccg gcatggtggc
     3241 ctactgcgac ttcgccaccc aggccacggt cgagaccgag gaggggcggc agcgccccga
     3301 cctggtggtg cggctgccga accagaagca gatcgtcatc gacgccaagg ccccgctctc
     3361 ggcctatctg gacgccctgg aggtccacga cgacgagggc cgccgggcga agctgcgcga
     3421 gcacgcgagg cagatccgca cgcacctcgc gaagctgtcg cagaagacct actgggcgca
     3481 gttcgagccg gcgccggagt tcgtcgtcct cttcctgccg ggggagatct tcttctccgc
     3541 tgccctggag caggacccgt cgctcatcga gctgggcgtc gagcagcggg tgatcctggc
     3601 gacgccgacg acgctgatcg cgctgctccg ggcggtggcc tatggctgga gacaggcgca
     3661 ggtggaggag agcgcgcgga cgatcagcga gctcggccgg gcgctccacg acaggctgcg
     3721 caccttcgtc gtccacctgg gcacgatgcg ccgcggcctc gaccaggcgg tggacgccta
     3781 caaccgcgcc gtcggttcgc tcgaggggcg agtcctgccg gccgcccgcc gcttcaagga
     3841 cctgggcgcg gccggcggcg atgagatcga gaccctggag agcgtcgaca aggtgacccg
     3901 ctccatcgaa tcggtcgaag ccgtcgatgc cgctgatctc tcgcaaagcc tgtaacagcg
     3961 gcctcccgga ggagcctatg gaggtaacgc cggcccggat acgttctcaa gactcccggg
     4021 gaccggcctc atctccgaac gccgaggagg ctgaaccatg tcatccagcg actcgaaagc
     4081 acagtcgttc gtccgccgcc gcgccctcat cctgggagtg gccgtcatcc tgggcaccct
     4141 cgccctcgat gtccaggccg agcccattcc ggacggcgcc ctggcgccgc accgcgtcgg
     4201 ctggttcgac aacggcggca ccatgacgtg tgccgagacc tgcaaggcaa aggcgcccgg
     4261 tagctcagcc gagcacgagc cgagccccat cgcgccgacc aagcgggtct tcgtgtgcaa
     4321 ggttgcgggt aagcccaacg gcgatctccg gacctggctc tacggcaatc agttcgacga
     4381 ccgccccgcc tgctacacga ccggcctcga tctcaagggc tcgtactcca agaagttctc
     4441 ctgcctctgc gtcaggtgaa agccatccaa caaaaagccc cggccggagc cggggctttt
     4501 tgcttcagcg gccggaggcc ggggtcagat gaaggtcttg atcttcgcct tgctgcggag
     4561 ctggttgacg tgggcctcga cggcctcgcc ggtctgcttg gtgcgcagga aggtggtgag
     4621 ccggtccttg acctcggcga gcggggcggt gtcggctgcc tgcttctcct ggagctggat
     4681 gacgtggaag ccgaagcggc tctcgaccac tgggctgatc tcgttcggct tcagggcgaa
     4741 ggccgccttc tcgaacggct ccacggtctg gccaggggcc acccagccga ggtcgccgcc
     4801 gcgcggtttc gagttcgaat gaggctctca tacgttccgc caacgccttg cccactttgg
     4861 ggctcttttc cgcaaggcaa gcgggctcag gagacgagct gcgcgccggg tatggcctga
     4921 cttttagggt gggctgagct actataggtg ccaagaaaat gggtggtagt tcgttgccgg
     4981 atatgcttcg tagacaccgg ctggctactc gattgactcc aagagaggca aaatggctac
     5041 acttcgcgag cagctgacaa agattcttcc ttcggttctt ccggaccgag cggaggacgc
     5101 aatcaatgga actgatctcc tcgccacggt gaggggcaag ctcgacggcg attactctga
     5161 ggctagcgtt cggacgcact tctcagttat gtctgcagat ccgacctctc ccattgcgaa
     5221 ggtcgaccaa ggccatgggt actaccggcg ctccttgggg acaaccgcag cggctattcc
     5281 cattgacgaa ggtggagcga agagcgtgac tgaagaggcc gctgaagagt ttcttggaca
     5341 acgtgcgtcc cagcgggaag agaagttccg ctcaatctat atgaggtatg cagagatcac
     5401 aaatcagttt cccatgcatg tagagcatac taagggtgcg cggcagcgtg ccggcgtcaa
     5461 caagtggaag ttcccagatg ttgtggtact tcggtgggag gttggagagg tctctgatgc
     5521 ggggttccgt cttgctcgag aagtactcga ggttaaaaag agccttgggg aacagccatt
     5581 caagctaatc agttccgagc tcaaagtaga gcttgccatc gggtcctttc gtgaggcgtt
     5641 ttttcagtgc gtgagtaact cgaagtgggc gcactccgcc cagcttgcag tggcagcaaa
     5701 gataaatgat gagagccttg cagaggagct acggcgtctg ggtacatcat atgatgtctc
     5761 ggtagtgtct tacggtttag agccggaata ccttgattcg ttgcccccgg cagacaagat
     5821 cttgaaaatg caaacaggtg acttcgaggc gatagctgca aatgttgcgg taactcgtgt
     5881 cgctaccggg agagaccgag agacgctgga ctgggagcac ctccgtgatt tgcgcgccca
     5941 aagtgaagaa tttagtaatt tattccggtg gaccgcatac tgtctagaga agaaatcgcc
     6001 atacaggtat gaggactggg agaagatcgc acgtcttgag agccggtaca cataggtcag
     6061 caagcgatgc ctgatgaagg tgcccataaa ccgtccatca tcaggaggtg gggcgggtcg
     6121 gagcctcctc atcgatcgcc aactcgttcg gcaacaagcg atgatgcttg cggatcacgg
     6181 aatccagggt gcacagagga attctacgaa ccgagctgtg ggcggcgagc aaccggtcga
     6241 aggggtcgcg tgtccaggtc agctcgatcg cgtggcggac caatggctgg aggggcgcct
     6301 catccaggag gaatcgctga tccccggtga ccgtctcggt aaaacccgga ttgcggattc
     6361 ctatccggcc cacttcggcc agaaactgga tctcgagcag ggagacgggc gagatccccc
     6421 agggatgata gcgatcgagc caggggaact catcgagccg agcagacccc atggtcagcc
     6481 agatcagaaa gtgcgtgtcc agtagcagct tcaccggtcc cggtccactg gcgagagagc
     6541 ttcgcccggc tccttccact cccaccccca gcggtccgcg tcttcagcgt ggggcgccca
     6601 gatcaggcct tcgtagctag ggacgtggcg gggtgcttcc tcctcccgag cttcgcggtg
     6661 cagcaccaat cgcgtgcctc cccgttcgat cagcagaacc tcgccctcgg cagcttcgtc
     6721 gaggtaacgg aaccagttct tccgggcttc tgtcgcggtg actcgcttca tgtgtacagt
     6781 gtacacttcc tggaatgaaa agccccggcc ggagccgggg ctttttcctt cagcggccgg
     6841 ccgccggggt cagatgaagg tctcgatctt ggccttactg cggagctggt tgacgtgggc
     6901 ctcgatggcc tcgccggtct gcttggtgcg caggaaggtg gtgagacgct ccttgacctc
     6961 ggagagcggg gcggtgtcgg ccgcctgctt ctcctggagc tggatgatgt ggaagccgaa
     7021 gcggctctcg accaccgggc tgatctcgtt cggcttcaag gcgaaggccg ccttctcgaa
     7081 cggctccacg gtctggccgg gggccaccca gccgaggtcg ccgccgcgcg gcttggagcc
     7141 cgggtcgtcc gacgcctcct gggccacctt ggcgaagtcc tctccgccct tgacgcgggc
     7201 gaggagcccc tcggccttct gccgggccgc ctgcttggcc tcggcagtgg cgttctgctc
     7261 cacccgcacc aggatgtggc gcaggcgggc gcgctcgggc cgcttgaact cgtccttgtt
     7321 ctggtcgtag taggcctgga tgtcctggtc ggtgaccttg acggtgctga ccaccttgct
     7381 ggcgaggagc ttctcgacca cggcgttctt gcgcacctcc tgcttgaact tgtcctcggt
     7441 catcttctcg gtggtcagag cctgcttgaa cttctcgtcg cccatccgct tgcgcagcgg
     7501 ctcgatctgg gcgttcacct ccgcctcggt gaccgtgatc ccctgagctt gagcctccgc
     7561 gaggaggagc tgctgggcga ccatgccgtc caggatcttc tggtagaact cctgggtcgc
     7621 cggcgtctgc tggccgagcc gcgccgcgaa ctgctgctgc atccgctcgg cctcggcgag
     7681 gagggcgtcc ttgcggatct cctggccgtt gatcctggcg accacggcgg ggatcttgtc
     7741 agccggcatg ggcggggcgg cggcggccgg ggcgctcccc ggggccgcga cctggcccgg
     7801 cagcggctgg ccctgggggg ccacggtcgc gccgggggcc ggagcggcgg tcccgggctg
     7861 ggcctgggtc tgcgcttggc cctgcgcctg ggcatcggcc ggctcgcctt cggaggcggg
     7921 gctgcaggcg aggagcccgg tgaccacgaa ggtgagcgcc agggacatca atttcgcggg
     7981 gtacgaggtg cggttgtcga tcatggatgg cttcctttga cgggcgggac ggtacgcccg
     8041 ccggggcgat atggggcggt cttgggcgat tccagacgtc cagccgcgga tcttcggccg
     8101 cggctcagaa ggatattaac cggaacggac agggacttta ttcgattcca ctcccaaaga
     8161 cgagggggac ccgttccgga gctgccggcc ggcgatcagg tggagggccg aggcgtccag
     8221 gacggcgcgc aggagagcgc ccagcgcggc cccctcggct ccccactgcc ggatcagcag
     8281 cgcgccggcc gggaggaaga ccagcagctc ggccagccgg agccgcgcgg tgaggtccgg
     8341 ccgtcccagg gcctggagga ccgcggcgga gacgctcccc agcgccgaga agagcaggcc
     8401 gatggcgagc aggcgcagga ccgcgg
//
LOCUS       DASPJI010000405        13131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_140258, whole genome shotgun sequence.
ACCESSION   DASPJI010000405 DASPJI010000000
VERSION     DASPJI010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13131)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13131
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140258"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..975)
                     /locus_tag="VEL74_10880"
     CDS             complement(142..975)
                     /locus_tag="VEL74_10880"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HYG63076.1"
                     /translation="MRYDRRVYLLPPDLEDLLVAELWSAGTAGVESAPEKDGRVRLTA
                     YFPAGEDPELETPPGVELVADEPVPETDWLAVYRERAKPFPVARTLFVDPREPDEPPA
                     EVPEGRTLLRLPARAAFGTGSHESTGLALELLEAAGVRGRRVLDVGTGTGVLSFAALH
                     FGARLAVAFDVDPAAPFHGRENSRLNDLHPLFFAGRLAALAPRPLFDLALVNIVPEQI
                     LPEMGTLDRLLGPAGEAILSGILTERGGQVLARMQGLGFAEIDRRVGGDWIAFRVRRR
                     T"
     gene            1190..1402
                     /locus_tag="VEL74_10885"
     CDS             1190..1402
                     /locus_tag="VEL74_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63077.1"
                     /translation="MSWRARVRLMVLCVVLGVVSAAQTMALTAMKQTWCDQSGCGCAQ
                     TSNGQNLLTFACGCDSTGSWRTCTYG"
     gene            1556..3595
                     /locus_tag="VEL74_10890"
     CDS             1556..3595
                     /locus_tag="VEL74_10890"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYG63078.1"
                     /translation="MQFQVKDSKAPVVWKGGPEGLALVCFGGEGIAVSCEQMYLARNA
                     SVPPVLQKGVRITALVRIGESASQGAKVALIPLHLRARSYDVFLPLGKDEKSGKMVRE
                     LVSDRSGRFTTPQLAPGDYLFLITSQDGRTLRHEVRVPRPEALRSGKKPPEPPTEPLL
                     DIGEIVFPRGVTLEAWVTARDGRPLRSATFAARQGPQMGEPRNFEAVTNAEGKAKLVG
                     LDPAAETRLTCQAKGFASFENRYDVPPPALSCVLDPLSRIDGEVVDLEGGPVAGATIS
                     NFEYGRLAATGDDGTFSIPEIDIPQLDLTIAAPGFRVDRRTVEIGPGEEKKLTVELRP
                     GQEIRGLVRDAVTREPVSGAVIVSTEPLGAVSTLSDSEGAFDISTDSEGTLWLEVSSE
                     GYPKTRVSLEPQRIATLAGKDEPLVVDLKEGGRVRVAVWDEEADAPCQGCGVLVGGET
                     IVTDRNGEGLSGLIAEGSHLAILSQVKNWGGEVQVQGGGITKQVDVVAGQITPIRLGE
                     PRSTLEVHFRPRVPEGWTLRAESAMEPGSTERLSNGAFKVRKPKSGPLLLRLGLADGL
                     RDGVTWIDQAIVPEDYTSPVLDLPLPQGGVRGKLIKEGEVPVAGRMLEIFSTSSGTRR
                     AYTYSRGDGSFLIPFLPAGVYAVVVDGQAIRPFSLEEGADMDLGSVELPSAPTRR"
     gene            complement(4187..4606)
                     /locus_tag="VEL74_10895"
     CDS             complement(4187..4606)
                     /locus_tag="VEL74_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG63079.1"
                     /translation="MIVLDTNVVSALMRSEPDPIVVEWLDSLPPESIWTTSVTVFEVR
                     LGLEILVAGRRRRQLEDAFEKALEEDFENRVLSFDQAAAQVAGRIAAERRRAGRTVEI
                     RDLQIAGIAKVRKATLATRNTRHFEGCGLVLLDPWSA"
     gene            complement(4603..4848)
                     /locus_tag="VEL74_10900"
     CDS             complement(4603..4848)
                     /locus_tag="VEL74_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011778953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system"
                     /protein_id="HYG63080.1"
                     /translation="MAQLLVRHLDDDVKAKLQLRARRHGHSTEEEAREILRNAVREEG
                     SARPPLGSRLAGRFAGLGLTEDVPELRGEQARPADLE"
     gene            complement(5192..5449)
                     /locus_tag="VEL74_10905"
     CDS             complement(5192..5449)
                     /locus_tag="VEL74_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG63081.1"
                     /translation="MHNETVTPISTQDPFAGLGEALHRLRKTRGLSQTDLASQTGLAH
                     GQVSAIEAGKQKPSLESLSKLMRTLGCDLDELSSALKSVRG"
     gene            complement(5561..6679)
                     /gene="dnaJ"
                     /locus_tag="VEL74_10910"
     CDS             complement(5561..6679)
                     /gene="dnaJ"
                     /locus_tag="VEL74_10910"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HYG63082.1"
                     /translation="MASKRDYYEVLGVAKTASAQEIKSAYRKIAVRDHPDRNPDNKEA
                     EERFKEAAEAYAVLSDADKRARYDRFGHQGVQGAGAGGFDPTIFADFSDILGDFFGLG
                     GGFGRRGGGSGMARGADLRYDLTLNFEEAAFGHETTLRIPRLETCGKCSGHGSADGSA
                     PKTCTACGGRGQVRFTQGFFTVARTCPQCQGEGQVVSNPCPECKGEGRVERERSIVVK
                     IPAGVDTGARLRLAGEGEHGRRGGPPGDLYVVLQVRPHARFRRDGSTVFSRESISYPQ
                     AVLGTTLEVDTIHGKAPLEIPAGTANGRDFRLRSQGISRLDGSGKGDHVVTIEVAVPH
                     PKDLGDEEVTLLRRLAEIGGHPVKEERTVLDRVKNLFG"
     gene            complement(6695..8524)
                     /gene="dnaK"
                     /locus_tag="VEL74_10915"
     CDS             complement(6695..8524)
                     /gene="dnaK"
                     /locus_tag="VEL74_10915"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HYG63083.1"
                     /translation="MSKILGIDLGTTNSCASIVEATSPRVLANREGSRTTPSIVAFAE
                     EGDRLVGQIAKRQAITNPENTVFAVKRLIGRKYNDPQVAKARGVLPYTLGQAGNGDLK
                     IEIRDRQYSPEEISAQILREIKEFAEEALGEEVKEAVITVPAYFDDSQRQATKDAGRI
                     AGLEVQRIINEPTAAALAYGLDRREGGSGTIAVYDLGGGTFDVSVLELSKGIFEVRST
                     SGDTYLGGEDFDQRIMDWLIQEFLRDTGIDLKADRMALQRLKEAAERAKCELSSAEEA
                     AINLPFISADRSGPRHLARNLTRAHFEALVADLIERTEGPCRDALKAAGLRADQIDEV
                     LLVGGQTRTPAVIRAVERIFGRKPNGDINPDEVVAMGAAIQGGILRGDIKDLVLLDVT
                     PLSLGVETQGGLFTRLIERNSTIPTKNTQIFTTVVDNQDTVEIHILQGERQIANENRS
                     LGRFELVGIPPAPRGVPQIEVTFALDSNGIVNVSARDLATSLSQSVQVTPAGGLSEDE
                     VSRLVEEADKQSQQDLQKREVQQIKNRLEGLIYSNERVFEQFRDMLQEADSKRIHEAL
                     LQARMAVAAEQRAELEAAIFDLNTVSRTLSDVMLTKAGGGPKA"
     gene            complement(8714..9388)
                     /gene="grpE"
                     /locus_tag="VEL74_10920"
     CDS             complement(8714..9388)
                     /gene="grpE"
                     /locus_tag="VEL74_10920"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HYG63084.1"
                     /translation="MADDTTERNEAAGQDVAELDLDAAQNEDLDAAMRAAVAAVEEVE
                     SGGRHEEAAAGGGSGDGDTSELFMLRREVADLRNRSMRTLADFDNFRKRSERERQEAR
                     RYALFEPLRDFLDVVDNLDLALASEGSVEDLKRGVEMIQRQMQELLRRHGVKEVPAAG
                     EPFDPAVHEAVSREEDPAVKAPTVSAELRRGYMMHERLLRPAMVKVAVPAEGARPSGE
                     GAGDGE"
     gene            complement(9394..10467)
                     /gene="hrcA"
                     /locus_tag="VEL74_10925"
     CDS             complement(9394..10467)
                     /gene="hrcA"
                     /locus_tag="VEL74_10925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HYG63085.1"
                     /translation="MNDPESRGKLSPRDQEILRDVIVTYILSAEPVSSRSVAKRGRLG
                     LSAASIRNVMADLEEMGYLAQPHTSAGRVPTRAAYHLFIQSLMEERQLTPSERRYIDE
                     TLHGRPADADELMGVTSHLLSEMSNQVGLVVAPVMGETVLKTVDFVPVADRKVLCVVV
                     STTGFIDNKVIETDEEIPREELVRISNYLTDNFAGQTLRQIRDRLLRTMAEERAQVDR
                     MLARTIDLARKGLEERPGQEVLMDGTSALLAQPELSDIHRVRRMFEAFADKARLVQML
                     NQCMSGDGVRVLIGDDNALTSELDFSLVAAPYGVGDRHLGTLGIFGPSRMEYQKIIPL
                     VHYLGERLSRALSESFSGGPDRR"
     gene            10705..11568
                     /locus_tag="VEL74_10930"
     CDS             10705..11568
                     /locus_tag="VEL74_10930"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HYG63086.1"
                     /translation="MSEPLAGVRFSLVGPGKVGTSLAAWAVASGARLMSTAGRVSLEE
                     MESAGQDLLLVAVSDSALPAVAATLAGRPQAAVALHTSGALDASVLSPLRQAGSAVGS
                     LHPLKAFPRPLPDPAQARGVFFGVDGDPAARAMAGRLAEAWGAVAGEVPAEARLLYHF
                     AATLAAGGVVTLLAAAEDLAGRLGLPPQVTAGYLELSRGAMAAALETGAAGDSLPGAI
                     TGPVARGDVGLVLRQLDALRRTDPGKLPLVLQLARETLRQVSRRAPLDPGQEELREEI
                     ERLLRDPLSPH"
     gene            11609..12187
                     /locus_tag="VEL74_10935"
                     /pseudo
     CDS             11609..12187
                     /locus_tag="VEL74_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
     gene            12780..>13131
                     /gene="nrdR"
                     /locus_tag="VEL74_10940"
     CDS             12780..>13131
                     /gene="nrdR"
                     /locus_tag="VEL74_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HYG63087.1"
                     /translation="MRCPFCAENHDRVVDSRESRDGVTIRRRRECMACGRRFTSYEQI
                     EDIPYMVVKHDGSREEFSRKKLLNGLLKACEKRPVPVRKLESIVDETESMLHDREDRE
                     ISTTEIGAFVMDRLR"
BASE COUNT         1879 a         4620 c         4590 g         2042 t
ORIGIN      
        1 cccagcgggc gtggccgcgg ccatccacca cccgcagctt ctcccccacc tcggcgcgcc
       61 gggcgcggaa caggtggcga taggggtccc cctcgacgcg gacctcgtcc ccttcgaagc
      121 cggtctcggc gagcagggtg atcatgtacg acgcctcacc cggaaggcga tccagtcgcc
      181 gccgacgcgc cggtcgatct ccgcgaagcc cagaccctgc atgcgggcga gcacctggcc
      241 gccgcgctcg gtgaggatcc ccgacaggat cgcctcgccc gccgggccga gcagccggtc
      301 gagcgtcccc atctccggca ggatctgctc cggcacgatg ttgaccagcg ccaggtcgaa
      361 gagcggcctg ggcgccagcg ccgcgaggcg gccggcgaag aagagcgggt ggagatcgtt
      421 gaggcggctg ttctcacggc cgtggaaggg ggcggcgggg tcgacgtcga aggcgacggc
      481 gaggcgggcg ccgaaatgga gggcggcgaa ggagaggacg ccggtgccgg tcccgacgtc
      541 gaggacgcgc cggccgcgca cgccggccgc ctccagcagc tccagagcga ggccggtcga
      601 ctcgtggctg ccggtgccga aggcggcgcg ggcgggcagg cgcagcagcg tccgcccctc
      661 cggcacctcg gccggcggct cgtccggctc gcggggatcg acgaacagcg tcctggcgac
      721 ggggaacggc ttcgcccgct cccggtagac ggcgagccag tcggtctcgg gcaccggctc
      781 gtcggcgacc agctcgacgc cggggggcgt ctccagctcc ggatcctctc cggccgggaa
      841 gtaggccgtg aggcgtaccc gcccgtcctt ctccggcgcc gactccaccc cggcggtccc
      901 ggcggaccac agctcggcga ccaggaggtc ttcgaggtcg ggggggagca ggtagacgcg
      961 gcggtcgtag cgcatggggg gcggccctcc tggagcgccc accttatata gaagagatgc
     1021 ccgcggggca acttcgcgtt gcggatcttg ccgcgcctgt cacgaaatct cttctccagg
     1081 ctaccaggaa gtttgaggga gcttcacttc ttttcttgct gttcctctga gaagccggcg
     1141 tcaacttccg caatcccgcg ggagggtctc gagttgggag gaggctgtca tgagctggag
     1201 agcgcgagtc cggttgatgg tgctgtgcgt ggtgctgggg gtggtgtccg cggcgcagac
     1261 gatggcgctg accgccatga agcagacctg gtgcgatcag tcggggtgtg gatgcgccca
     1321 gacttccaac ggccagaatc tgctcacctt cgcctgcggg tgcgactcca ccggcagttg
     1381 gcggacctgc acctacggct agcgcgggga gattctggtg cggaagcgat ctcgtatcct
     1441 actggcctct cttctcgtgg ctctgcccgg ggcgatcttt gcccaggggg caagcccgac
     1501 ggctccgccc gtttcctggg ccgagctccg gcctctcggc cagtccggag agcccgtgca
     1561 gttccaggtg aaggactcga aggcgccggt cgtctggaag ggcggccccg aggggctggc
     1621 gctggtctgc ttcgggggcg aggggatcgc cgtctcctgt gagcagatgt atctcgccag
     1681 gaacgcctcc gttcccccgg tgcttcagaa gggggtccgg atcaccgctc tggtccggat
     1741 cggagaatcc gcatcccagg gggccaaggt cgccctgatc cccctgcacc tccgagcccg
     1801 gagctatgac gtcttccttc ccctgggcaa ggacgagaag agcggcaaga tggtccgcga
     1861 gctggtgagc gaccgctccg ggcgcttcac caccccgcag ctcgcgccgg gggactacct
     1921 tttcctgatc acctcccagg acgggcgcac tctgcgccac gaggtgcggg tcccccggcc
     1981 cgaggccctg cggtctggca agaagccgcc ggagccgccg accgagccgc tgctcgatat
     2041 cggcgagatc gtctttcccc gcggggtcac cctcgaggca tgggtcaccg cccgcgacgg
     2101 ccggcccctc cgcagcgcca ccttcgccgc gcggcagggg cctcagatgg gagagccgcg
     2161 caacttcgag gcggtgacca acgccgaggg gaaggcgaag ctcgtcggcc tcgatccggc
     2221 ggccgagacc cggctcacct gccaggccaa ggggttcgcc agcttcgaga atcgctatga
     2281 cgtcccgccg ccggccctga gctgcgttct cgatcccctc tcgcgcatcg acggcgaggt
     2341 agtcgatctg gaggggggac cggtagccgg tgccacgatc tccaacttcg aatacggccg
     2401 gctggcggcc acgggcgacg atggaacgtt ctccatcccc gagatcgata ttccccagct
     2461 cgacctcacg atcgccgccc cgggattccg ggtggaccgc cggaccgtgg aaatcgggcc
     2521 gggggaggag aagaagctga cggtcgagct ccggccaggc caggagatcc gcgggctggt
     2581 gcgcgacgcc gtgacccggg agccggtgag cggtgcggtc atcgtctcca ccgagccgct
     2641 gggcgccgtc tccaccctca gcgactccga gggggcgttc gacatcagca cggattccga
     2701 gggcacactc tggctggagg tctcctccga gggctatccc aagactcgcg tgagccttga
     2761 gcctcagagg attgccaccc tggccgggaa ggacgagccg ctcgtggtgg atctcaagga
     2821 aggcggccgg gtgcgcgtcg ccgtctggga cgaagaggcc gatgctccct gccagggctg
     2881 cggcgtcctt gtcggtggag agaccatcgt gaccgaccgg aacggtgaag gcctatccgg
     2941 cctcatcgcc gagggatccc acctcgccat cctgtcgcag gtgaagaact gggggggaga
     3001 ggtgcaggtc caaggcggcg gcatcacgaa gcaggtcgac gtggtcgcgg gccagatcac
     3061 accgatccgc ctcggggagc cccggtcgac gctggaggtc cacttccgtc cccgggtgcc
     3121 agaaggttgg accttgcggg ccgagtccgc tatggagccg ggaagcaccg agcgcctttc
     3181 caacggcgcc ttcaaggttc gcaagcccaa gagcgggccg ttgctgctac ggctcggttt
     3241 ggcggatggc ttgcgggatg gcgtgacatg gatcgatcag gccatcgtgc cggaggacta
     3301 cacgtctccg gtgctcgacc tgcctcttcc tcaagggggc gtccgcggca agctgatcaa
     3361 ggaaggcgag gttccggtcg ccggacgcat gctcgagatc ttttcgacct catcgggcac
     3421 ccgccgcgcc tacacctact ccagaggcga cggctcgttc ctcatcccct tcctgccggc
     3481 tggcgtctac gccgtcgtag tggatgggca ggcaatccgg cccttctctc tggaggaggg
     3541 cgcagacatg gacctgggga gcgtagagct gccgtcggcg cctacgcgcc gctgaaccag
     3601 ccgcgaagcg acgcacggat cgctccgctc cggccgctcg ccgagaggcc agagcgcgtg
     3661 cggctccggc cagtccggcc actcctgcga ggaggtgggc aggatcaccc agggaccctc
     3721 cccctcgggg tcgtcccccc gggcggctcg tcccgtcccg atccgcagct tcggacacgc
     3781 tccacacaga aaccgcggcc atttccccgg aacagccgtc gcccgcgcag agacgtccgc
     3841 cgtccttcgg ggtacggcct tcctacccgg gaaacggaca ccatccccgc gggtacggac
     3901 tccatcgcca ggggtacggt gccatcccct caggtacgga ctccgtaccc gaggagatga
     3961 caatcatgcc gtgggtacgg ccttcctacc cgcggggaga ccacgatccc ccggggtacg
     4021 gaggtccgtc gccgggggat ggcgatgatt tcggcgggaa tgatgaccgt tccgaggcgg
     4081 atgacggtcc tctcgccggg aacgatgttc acggcgagtg ggaaagcggc aatctcacca
     4141 gggacgagct cggcaaggcg gcgcgaaccg ccgtttcgaa ggcgagtcac gccgaccagg
     4201 ggtcgaggag caccagaccg cagccttcga agtggcgagt gttgcgggtc gccagggtcg
     4261 ccttgcgtac cttggcgatg ccggcaatct ggagatctcg aatctcgacg gtgcggccgg
     4321 cacggcgtcg ctccgcggca atgcggccgg cgacctgagc cgcagcttga tcgaaggaca
     4381 ggacccggtt ctcgaagtcc tcttcgagcg ccttctcgaa ggcatcttcc agttgacgcc
     4441 tgcgccggcc cgccaccagg atctccaagc ccaagcggac ctcgaagaca gtgaccgaag
     4501 tcgtccatat ggactcgggc ggcaggctgt cgagccactc gaccacgatc ggatcgggct
     4561 cgctccgcat cagagccgag accacgttgg tgtccagcac aatcattcga ggtcggccgg
     4621 ccgggcctgc tccccgcgca attcgggaac atcctcggta aggcccagac cggcaaagcg
     4681 ccctgccagc cgggagccga ggggcggccg agcgctgccc tcctccctga ctgcattgcg
     4741 gaggatctcg cgcgcttcct cttcagtgct gtgaccgtgg cggcgggcac ggagctgaag
     4801 tttggctttg acgtcgtcgt cgaggtgtcg aaccaggagt tgagccatag cggaatgata
     4861 tcacgatatc aatcatggcg agatgtgtgc caatggacgg caacgccgtg cccatcatcg
     4921 gcagaccgct ccccgtggca ggccgcgcgc ggccgacgac aggccgcgcc gtgccgacga
     4981 cgggccgcgc ccagccgcca gcaggcaagg ccgcgccgat gaccggccgc gccgtgccgg
     5041 caaccggccg agccctcccc aggacgggcg ccgcgtggcc agccgtcggc ggagtttttc
     5101 cgaggatcgg gagagcgggg ccgacatctc ctccggcggc ggcgcctcgc ggcgccgccg
     5161 cccgatcggt cctgcaagaa gaggggatgc ctcagccgcg gacgctcttg agcgccgagc
     5221 tgagctcgtc caggtcgcag ccgagggtgc gcatcagctt gctcaggctc tcgaggctcg
     5281 gcttctgctt gccggcttcg atcgcggaga cctggccgtg ggcgaggccg gtctggctgg
     5341 cgagatcggt ctgcgacagg ccgcgcgtct tgcgcagacg gtgcagcgcc tcgccgaggc
     5401 cggcgaacgg gtcctgcgtc gaaatgggcg tgacggtttc gttgtgcatg gtggcaatct
     5461 accaccaagt ttccgggacg gcaacagggc gaggagtcgc ccgggggcgc ggattcgcac
     5521 ccccggagac gctcgccttc tgaagagaga tgccggcggg tcagccgaag aggttcttca
     5581 cccggtcgag gaccgtccgc tcctccttca ccgggtggcc gccgatctcg gccagccggc
     5641 gcaggagcgt gacctcctcg tcccccaggt ccttggggtg ggggaccgcc acctcgatgg
     5701 tgaccacgtg gtcccccttg ccgctgccgt ccagccggct gatcccctgg ctgcgcagac
     5761 ggaaatcgcg gccgttggcg gtgcccgccg ggatctccag cggcgccttg ccgtggatgg
     5821 tgtccacctc cagggtcgtc cccagcaccg cctgcggata gctgatcgac tcgcggctga
     5881 acaccgtcga gccgtcgcgg cggaagcggg catggggccg cacctggagc accacgtaga
     5941 ggtctcccgg cggaccgccg cggcgcccgt gctccccctc gcccgcgaga cgcagccgcg
     6001 cgccggtgtc gacgccggcc gggatcttca ccacgatcga gcgctcgcgc tccacccgcc
     6061 cctccccctt gcactcgggg caggggttcg acaccacttg gccctcgccc tggcactgcg
     6121 ggcaggtccg cgcgacggtg aagaagccct gggtgaagcg cacctggccc cggccgccgc
     6181 aggccgtgca ggtcttcggc gccgagccgt cggcgctgcc gtggcccgag cacttgccgc
     6241 aggtctcgag gcgcgggatg cgcagcgtcg tctcgtggcc gaacgccgcc tcctcgaagt
     6301 tgagcgtcag gtcgtagcgc aggtcggcgc cgcgggccat gccgctgccg ccgccgcgcc
     6361 ggccgaagcc gccgcccagg ccgaagaagt cgcccaggat gtcggagaag tcggcgaaga
     6421 tggtcggatc gaagccgccg gcgccggccc cctgcacccc ctggtggccg aagcggtcgt
     6481 agcgcgcccg cttgtcggcg tccgagagca ccgcgtaggc ctcggccgcc tccttgaagc
     6541 gctcctcggc ctccttgttg tcagggttcc ggtccggatg gtcgcgcacg gcgatcttgc
     6601 ggtacgcgga cttgatctcc tgcgcggagg cggtcttcgc cacgcccagg acttcgtaat
     6661 agtctcgttt ggaagccatt cctgaaacct tcggctacgc cttgggaccg ccgcccgcct
     6721 tggtcagcat gacgtcggag agtgtccggg acaccgtgtt gagatcgaag atggcggcct
     6781 cgagctccgc ccgctgctcg gccgccaccg ccatccgcgc ctggagcagc gcctcgtgga
     6841 tcctcttcga gtcggcctcc tgcagcatgt cgcggaactg ctcgaagacc cgctcgttgc
     6901 tgtagatgag gccctccagc cggttcttga tctgctggac ctcgcgcttc tggaggtcct
     6961 gctgcgactg cttgtccgcc tcttccacca gccggctcac ctcgtcttcc gagaggccgc
     7021 ccgcgggagt gacctggacg ctctgcgaca ggctggtggc caggtcccgc gccgatacgt
     7081 tgacgatgcc gttggagtcg agcgcgaagg tgacctcgat ctgcggaaca ccccggggcg
     7141 ccggcgggat tcccaccagc tcgaagcggc cgagcgagcg gttctcgttg gcgatctggc
     7201 gctcgccctg caggatgtgg atctccaccg tgtcctggtt gtccaccacg gtggtgaaga
     7261 tctgggtgtt cttggtcggg atcgtcgagt tgcgctcgat cagccgggtg aagaggcccc
     7321 cctgggtctc gacccccagc gacagcggcg tcacgtcgag cagcaccagg tccttgatgt
     7381 cgccgcgcag gatgccgccc tggatggcgg cgcccatggc cacgacctcg tcggggttga
     7441 tgtcgccgtt cggcttgcgg ccgaagatcc gctcgacggc gcggatcacc gccggggtgc
     7501 gggtctggcc gccgaccagc agcacctcgt cgatctggtc cgcgcgcagg ccggccgcct
     7561 tcagggcgtc acggcagggg ccctcggtgc gctcgatgag gtccgccacc agcgcctcga
     7621 agtgggcacg ggtcaggttg cgggcgaggt ggcgcggccc cgagcggtcg gccgagatga
     7681 acggcaggtt gatcgccgcc tcctccgccg aggagagctc gcacttggcc cgctccgccg
     7741 cctccttgag gcgctggagg gccatgcggt ccgccttgag gtcgatgccg gtgtcgcgca
     7801 ggaactcctg gatcagccag tccatgatcc gctggtcgaa gtcctcgccg ccgaggtagg
     7861 tgtcgcccga ggtggagcgc acctcgaaga tgcccttgga gagctccagg accgagacgt
     7921 cgaaggtgcc gcccccgagg tcgtagacgg cgatggtgcc gctccccccc tcgcgccggt
     7981 cgaggccgta ggccagggcg gcggcggtgg gctcgttgat gatgcgctgc acctcgaggc
     8041 cggcgatgcg gccggcgtcc ttggtcgcct ggcgctggga gtcgtcgaag taggcgggga
     8101 cggtgatgac cgcctccttg acctcctcgc ccagggcctc ctcggcgaac tccttgatct
     8161 cgcgcaggat ctgcgccgag atctcctccg ggctgtactg gcggtcgcgg atctcgatct
     8221 tgaggtcgcc gttgccggcc tgccccaggg tataggggag cacgccccgc gccttggcca
     8281 cctgcgggtc gttgtacttg cggccgatca ggcgcttgac cgcgaagacg gtgttctccg
     8341 ggttggtgat cgcctggcgc ttggcgatct ggccgacgag gcggtccccc tcctcggcga
     8401 aggccacgat cgacggcgtc gtgcggctcc cctcgcggtt ggcgagcacg cgcggcgagg
     8461 tcgcctcgac gatggacgcg cagctgttgg tagtccctag atcgatcccg agtatcttgc
     8521 tcacgcggtc tgcctcatcg agcccgttgg acggtgaagg acggctccga gggccggacg
     8581 ccctgactcc aggacgccgg gagcggacgg accgcccggg ccggcgcctg gagaatggcc
     8641 tctacctcgg agagctctac cttattgtaa aggctcgaag gaaagattgg gcgccggacc
     8701 gggcccggcg ctcctactcg ccgtctccgg cgccctctcc cgaggggcgg gccccctcgg
     8761 cgggcacggc gaccttgacc atcgccggcc gcaacagccg ctcgtgcatc atgtagccgc
     8821 gccgcagctc cgcggagacc gtcggcgcct tgaccgccgg atcctcctcg cgcgacaccg
     8881 cctcgtggac cgcggggtcg aacggctcgc cggccgccgg gacctccttg acgccgtggc
     8941 ggcgcagcag ctcctgcatc tggcgctgga tcatctcgac gccgcgcttc aggtcctcga
     9001 cgctcccttc ggaggcgagg gccaggtcga ggttgtccac cacgtcgagg aagtcgcgca
     9061 ggggctcgaa cagggcgtag cgccgggcct cctggcgctc gcgctcgctg cgcttgcgga
     9121 aattgtcgaa gtcggccagg gtgcgcatgg agcggttgcg caggtcggcg acctcgcgcc
     9181 gcagcatgaa cagctcgctg gtgtcgccat cgccactgcc gccgccggcg gccgcctctt
     9241 cgtgcctgcc gccgctctcc acctcttcca ccgccgcgac ggccgcgcgc atggcggcgt
     9301 cgaggtcttc gttctgggcg gcgtccaggt ccagctcggc cacgtcctgg cctgccgcct
     9361 cgttgcgttc ggtcgtatcg tctgccatcg ccgtcatctc ctgtcaggcc cgccggagaa
     9421 gctctcggag agggcccggc tcagccgctc cccgagataa tgcaccagcg ggatgatctt
     9481 ctgatactcc atgcgggaag ggccgaagat gcccagcgtc ccgaggtgcc ggtcgccgac
     9541 gccgtagggg gcggcgacca ggctgaaatc gagctccgaa gtcagggcgt tgtcgtcgcc
     9601 gatgagcacc cgcaccccgt ccccgctcat gcactggttg agcatctgca cgagccgcgc
     9661 cttgtcggcg aaggcctcga acatccgccg cacgcggtgg atgtcggaca gctcgggctg
     9721 ggccagcagg gccgaggtgc cgtccatcag cacctcctgc ccgggccgct cctccagccc
     9781 cttgcgggcg aggtcgatgg tgcgggcgag catccggtcg acctgggcgc gctcctcggc
     9841 catggtgcgc agcagccggt cgcggatctg ccgcagcgtc tgcccggcga agttgtcggt
     9901 caggtagttg gagatccgca ccagctcctc gcgcgggatc tcctcgtccg tctcgatgac
     9961 cttgttgtcg atgaacccgg tggtggagac gaccacgcag agcaccttgc ggtcggccac
    10021 gggcacgaag tccaccgtct tgagcacggt ctcgcccatc accggggcga ccacgaggcc
    10081 cacctggttc gacatctccg acagcaggtg ggaggtgacc cccatcagct cgtcggcgtc
    10141 ggcggggcgg ccgtgcaggg tctcgtcgat gtagcgccgc tcgctggggg tgagctggcg
    10201 ctcctccatg agggactgga tgaacaggtg ataggccgcc cgcgtgggca cccgcccggc
    10261 cgacgtgtgg ggctgggcga ggtagcccat ctcctccagg tcggccatga cgttgcggat
    10321 gctcgccgcc gacagcccca ggcgcccccg cttcgccacc gagcgcgagc tcaccggctc
    10381 ggcgctcagg atataggtga cgatgacgtc cctgaggatc tcctggtcgc ggggcgacag
    10441 cttgcctcgg ctctccggat cgttcatggg gccatctcgt tcagactctc tgggggattt
    10501 agcactcacc cgcggagagt gctaaggaat ggtagccgcg cccgccgcat ttgtcaaggc
    10561 aggaggagct aagtgactga gagtaaaggg tcctcaccgt gcctggggtt gacaccgccg
    10621 tcagccccgg gctgagggag gccctctcct ctacctcctc tatctatata gatgtcggcg
    10681 cgagaagcgg ctatcgtagg cggcgtgtca gagcccctcg ccggtgtgcg cttctccctg
    10741 gtcggcccgg ggaaggtcgg gaccagcctc gccgcctggg ccgtggcctc cggcgcgcgg
    10801 ctgatgagca cggccggccg ggtctccttg gaggagatgg agagcgccgg ccaggacctg
    10861 ctgctcgtcg ccgtgtccga ctccgcgctg cccgcggtgg cggcgacgct ggccgggcgg
    10921 ccgcaggcgg cggtggcgct ccacacctcc ggcgccctgg acgcctcggt gctctccccc
    10981 ctgcggcagg ccggctcggc ggtcggctcg ctccatcctc tgaaggcgtt cccccggccg
    11041 ctgcccgacc cggcgcaggc gcggggggtg ttcttcggcg tggacggcga cccggcggcg
    11101 cgggcgatgg cgggccggct ggcggaggcc tggggcgcgg tggccggcga ggtgccggcg
    11161 gaggcccgcc tcctctacca cttcgcggcg accctggcgg ccggcggcgt ggtgacgctg
    11221 ctcgccgcgg ccgaagacct ggccggacgg ctcggcctgc cgccgcaggt gaccgccggc
    11281 tacctggagc tcagccgggg cgcgatggcc gcggccctgg agaccggtgc cgccggcgat
    11341 tccctccctg gcgccatcac cggcccggtg gcgcgcgggg acgtcggcct cgtcctgcgg
    11401 cagctcgacg ccctgcggcg gaccgatccc ggcaagctcc ccctggtcct ccagctcgcc
    11461 cgggagaccc tgcggcaggt gtcccgccgg gcccccctgg accccgggca ggaggagctg
    11521 cgcgaggaga tcgagaggct gctgagggac cccctttccc cgcattagcc ttgtgctatg
    11581 cttcgaggcc cgtctcaggt tttcatttat ggtgttcttc aactacagca cgatgcagat
    11641 ggccgccaag gtcgtctatt acggccctgg tctctgcggc aagacgacca acctgcacta
    11701 catctacggc cacaccacgg gtgagtcgcg cggcgagatg gtgtcgctgg agaccgagac
    11761 ggaccggacg ctgttcttcg acctgctgcc gatcgacgtc ggcaacatcg ccggcttcgc
    11821 cactcgcatc cagctctaca ccgtgcccgg gcaggtgttc tacaacacca cccgcaagct
    11881 ggtgctcaag ggggtggatg gcgtggtctt cgtggccgac tcgcagcggg ccatggtgca
    11941 ggcgaacctc gactccttcc gtaacctgga ggagaacctg gcggagatgg ggctgtcggt
    12001 cgacaccctg ccgctggtcc tccagttcaa caaacgcgac ctgccggaca tctgcacggt
    12061 cgaggagatg aaccgctccc tcaaccggaa caactggccc tggttcgagg cgtcggcggt
    12121 caccggcgaa ggcgtcttcg agaccctcaa ggggatctcc aagcacaccc tgctggcgct
    12181 caagaagcgg ctcggccgcc ccgacgcgcc ggccacctcg gcccccggcc cggcccgccc
    12241 cgctccggcg cccgcggcgc gtccggcacc ggcgccaccg gcgccagcac cggccgcggc
    12301 ccctccggca ccggctccca ggccggtgcc ggtgccggcg ccgccgccgg cggcagcggt
    12361 ccgcccggcc cccgcgacgg caccggccgc cgccgctccg gccagacccg ccgctgccgc
    12421 gcccgccgcc aagtcgggcc gctcctccct ggatgccctg ggggagctgg agaagctgcg
    12481 caagcaggcc atgcagccgt cggcctcggc gccaccggcg gcgaagcccg cggctgcggc
    12541 ggccggctct ccggcgacca atggccgcgg cgagctgtcc cgcaccatcg agatgacgct
    12601 caagcggtcc gacctcgcgc gggcccgccg cttcctggtc agcttccagg tcgaggacga
    12661 ccagaaccag atcgtcgacg ccgtccgcga cctccgggtg gacatcaagg acgcggccca
    12721 cctcgaaaag ctcctcctgc gactcaacat cgccttgaac gccaaggagt gacctcggga
    12781 tgaggtgccc cttctgcgcc gagaaccacg accgggtcgt ggactcacgg gagagccgcg
    12841 acggcgtcac catccgccgc cgccgggagt gcatggcctg cggccgccgc ttcacctcct
    12901 acgagcagat cgaggacatc ccctacatgg tcgtcaagca cgacggctcg cgggaggagt
    12961 tcagccgcaa gaagctgctc aacggcctgc tcaaggcgtg cgagaagcgg ccggtgccgg
    13021 tgcgcaagct cgaatccatc gtcgacgaga ccgagagcat gctccacgac cgcgaggacc
    13081 gggagatctc gaccaccgag atcggcgcct tcgtcatgga ccggctgcgc g
//
LOCUS       DASPJI010000406         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_196862, whole genome shotgun sequence.
ACCESSION   DASPJI010000406 DASPJI010000000
VERSION     DASPJI010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196862"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..302)
                     /locus_tag="VEL74_10945"
     CDS             complement(138..302)
                     /locus_tag="VEL74_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63088.1"
                     /translation="MRSPLARAVLALVLFTAWLALLFAGFALGGAVHLLLAGSLALFP
                     WRSLRRAEGG"
     gene            339..1088
                     /locus_tag="VEL74_10950"
     CDS             339..1088
                     /locus_tag="VEL74_10950"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HYG63089.1"
                     /translation="MRIEAITFDVTHTLLHCPRLGEIYSEVLGRHGIPVSPPDALRLV
                     KTVWQELSCAADPGRDRFSSHPDGERGWWARFLERLCELLDAPPPSRFAAAELFHRFG
                     RAESWEVYPEVPRVLAELRGQGLRLAVISNWDHRLPGLLAELGLAPSFEAVVYSSAVG
                     VEKPDRRIFGRALELLQLAPAAVLHVGDGRLEDFEGAAAVGFAALHLVRQERGKVPAS
                     PGRGEILDLTALPGIVAARASGTRVLNSRQL"
     gene            1132..1620
                     /locus_tag="VEL74_10955"
     CDS             1132..1620
                     /locus_tag="VEL74_10955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HYG63090.1"
                     /translation="MKISTKGEYGIRAMLYVAMHAENGPVTSSAIARHQGIPEPYLRQ
                     ILAQLSKDRLIQSNRGPQGGHILARPAAQISMRHILMSLEGQTTHIDQILSLPCTIDV
                     GTEHCVIREVFLEVKKAVDRILSTTTLQDIVDRQREILQLGILVPHDVPASPSLLPVI
                     YE"
     gene            1643..1990
                     /locus_tag="VEL74_10960"
     CDS             1643..1990
                     /locus_tag="VEL74_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HYG63091.1"
                     /translation="MKREGEGLLVRIFIGEADRWDDLPLYEAIVQCARDKGLAGATVI
                     RGVEGFGAHNLIHTTSILSQDLPMVVEIVDQRDKIEAFLPELDGMISEGLVTLEPVHI
                     ILYRVRGPGDHAN"
BASE COUNT          348 a          819 c          788 g          338 t
ORIGIN      
        1 gcgctcgtgc ggccgctgct gcagcccatg gcgagcatca gcggcaggag caggaaggcg
       61 aagagggctc ggggcatggt cttgggtctc ctccggatca gggttcgagc acctgtgaaa
      121 aggcgagcga ttccctttca gcccccctcg gcccgccgca gcgagcgcca ggggaaaagg
      181 gccagggaac cggcgaggag gaggtggacc gctcccccga gggcgaagcc cgcgaagagc
      241 agggcgagcc aggcggtgaa gagaacgagc gccagaacgg cgcgggcgag cggcgagcgc
      301 acgccggcaa gctatcacag gagtatcctc taggggctgt gcgcatcgag gccatcacgt
      361 tcgacgtcac ccacaccctc ctccactgcc cccggctggg cgagatctac tcggaggtgc
      421 tcggccgcca cggcatcccg gtctccccgc ccgacgccct gcgcctggtc aagacggtct
      481 ggcaggagct ctcctgcgcc gccgaccccg gccgcgaccg cttctcctcc cacccggatg
      541 gcgagcgcgg ctggtgggcg cggttcctgg aacggctgtg cgagctcctg gacgcccccc
      601 ctccgtcgcg cttcgccgcc gccgagctgt tccaccgctt cggccgcgcc gagtcgtggg
      661 aggtctaccc cgaggtgccg cgggtcctcg cggagctccg cggccagggg ctgcgcctcg
      721 cggtcatcag caattgggac caccggctgc ccggcctgct ggcggagctg ggcctcgccc
      781 cctccttcga agcggtggtc tactcctcgg cggtgggggt ggagaagccc gaccggcgga
      841 tcttcggccg ggcgctcgag ctgctgcaac tggcgcccgc ggcggtgctc cacgtcggcg
      901 acggccgcct ggaggacttc gagggggccg cggccgtcgg cttcgcggcg ctgcacctcg
      961 tccgccagga gcggggcaag gtcccggcgt cgccgggccg tggcgagatc ctcgacctga
     1021 cggcgctgcc cggcatcgtg gcggcccggg cgagcggcac ccgtgtgcta aactcgcgac
     1081 agttgtgaga cgaggacacg ccttcccccc tcgcacaccg gacggccctc catgaagatt
     1141 tcgaccaagg gtgaatacgg tatccgggcg atgctctatg tcgccatgca tgccgagaac
     1201 gggccggtca ccagcagcgc gatcgcgcgc caccagggga ttcccgagcc ctacctgcgg
     1261 cagatcctcg cccagctctc caaggaccgg ctcatccagt cgaaccgcgg gccgcagggg
     1321 gggcacatcc tcgcccggcc ggccgcgcag atctcgatgc gccacatcct gatgtcgctg
     1381 gaagggcaga cgacccatat cgaccagatc ctctccctgc cgtgcaccat cgacgtcggc
     1441 acggagcact gcgtcatccg cgaggtcttc ctcgaggtga agaaggcggt cgaccgcatc
     1501 ctctccacca ccaccctgca ggacatcgtc gaccggcagc gggagatcct gcagctcggc
     1561 atcctggtgc cccacgacgt gcccgcgtcg ccgtcgctcc tgcccgtcat ctacgagtag
     1621 accgcggagg tccgttccat ccatgaagcg tgaaggcgaa gggctgctcg tccgcatctt
     1681 catcggcgag gccgaccgct gggacgacct gccgctctat gaggccatcg tccagtgcgc
     1741 ccgcgacaag gggctggccg gcgccacggt gatccgcggc gtcgagggct tcggcgccca
     1801 caacctcatc cacaccacca gcatcctctc ccaggatctg cccatggtgg tggagatcgt
     1861 cgaccagcgg gacaagatcg aggccttcct cccggagctc gatggcatga tctccgaggg
     1921 gctggtcacc ctggagccgg tccacatcat tctctatagg gtccgcggcc ccggcgacca
     1981 cgcgaactag ggccggccga gcagggccgc atgggcttcg ctcgggcccc gcttgtgaaa
     2041 ctcggcacaa gctgctagac tccgccgcgc ctggagaggg tgtgtgcgcg tgcgcgctcc
     2101 ggacccggag gacgcgatgc ccgaagaaca gaagcgatac ccgagctggg tccgcctgtc
     2161 cggaatcggc gtcgagctgg tcggggccgt ggtgggcttc acgctggtgg gctactggat
     2221 cgaccgccat ttcggcagca gcccgtgggg cctgctgatc gggctcggcc tgggactcgt
     2281 cggcggcatg tac
//
LOCUS       DASPJI010000407         5065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_22829, whole genome shotgun sequence.
ACCESSION   DASPJI010000407 DASPJI010000000
VERSION     DASPJI010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22829"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2765)
                     /locus_tag="VEL74_10965"
     CDS             complement(<1..2765)
                     /locus_tag="VEL74_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63092.1"
                     /translation="MCGISGPVRVGGWFACLACLVLAAAPAAASQVSLVSRAEPTLPN
                     DAGIGFGAGRPSSDGRFVVFLSAEAHLVPGQVDTNRTTDVFLRDRATGTTVLVSRSAT
                     SPTTAANDSSTAPVLSADGRYVAFSSLATDLVAGQTDNNFLADLFLFDRVTGTVTLVS
                     HAASSPATTGNHTAGGAVSLSADGAYIAFTSPAGNLVAGQGGGAGVQVFLYDRAAGTN
                     RLVSHAAGSATFASAAGADRPTLSADGRWVVYESGSEDLVAGSDLPATAVFLFDRTTG
                     TNILVSRSSGSMTQGGDGASTSPRISADGLWVAFQSTASNLVAGVTGSSSQDIFLWER
                     QTGAMTLVTHDASSASTEAGVFSFLAGISADGGRVLFSALSTNLVPGQSGPAGLTKIF
                     LFDRATGANTLVNHAAGSPAAVADQGDFRAFLSGDGRFVAFTTSARNILPGAPETNLD
                     DDVFLYDHAGGGTSLASRVAGTAAAYPQGNSVGSGISDGGEHIVFVSTVANLASGVRD
                     FNGIEDVYTHDRVAGTAELVSRLASPSLSAGAFTDSTDLPTLTSTDGRWVVFTSSAPN
                     LVPGQVEDEAAPGATRDVFLHDRATGTTLLVSRKAGATPATAASNSSSNPVISADGRW
                     VAFQSLAEDLVADQAQSWTDYFNVFLYDRDTGIITLLSHAAGLPSTSANNHSGLPSIS
                     ADGSRILFRSLATDLGATDGNGMSDLFLYDRAAGTRTLISHAAGAPATTANGPSTYGR
                     LSADGAFIVFTSWASDLLAGQTDPSYSEDVFLHQIAAGTTTLVSHIPGSATTVAAGDS
                     RQPVISADGAVVAFESFAGDLVAGQTDTNGGYDVFLFARATGVVTLVSRASGSATTTG
                     DASSWQPVLSADGAWLAFHSFASNLVAGQVAGLSTFEGDVFLHERATGQTRLMSHAPG
                     APLVAAAS"
     gene            complement(2951..3172)
                     /locus_tag="VEL74_10970"
     CDS             complement(2951..3172)
                     /locus_tag="VEL74_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63093.1"
                     /translation="MRKLVLLSLALVFAVAGVVTPPAATAGRCDYNNCVTYCSSPNAC
                     GYVCCYQQCCGDCCIDLLCVPPPPCEGEN"
     gene            3285..>5065
                     /locus_tag="VEL74_10975"
     CDS             3285..>5065
                     /locus_tag="VEL74_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYG63094.1"
                     /translation="MALSDFHPAVSRWFEKSLGSPTAPQAQAWPEIRARRHTLIAAPT
                     GSGKTLAAFLAAIDDLVRQALVGEDGLPDETEIVYVSPLKALSNDIQKNLQEPLEGIR
                     RELAAAGLPDVEIRTLVRTGDTPASERAAMLKRPPHILVTTPESLYILLTSEGGRGIL
                     KTTRTVIVDEIHAVADDKRGSHLSLSLERLEALCNKEGRSLVRVGLSATQKPIEEVAR
                     FLVGTANVEADGTPRCAIVDAGHGRRLDLCLEVPLSPLEAVMPNEVWDEVYDRLAGLI
                     RQHETTLVFTNTRRTAERVTRHLSERLGPGKVTSHHGSLAKEQRLESERRLKAGELSA
                     LVATASLELGIDIGSVDLVCQLGSTRSIATLLQRVGRSGHHLGGLPKGRIFPLTRDEL
                     VECTALLAAVRRGELDRLVIPERPTDVLAQQIVAAVAAEEWEEDDLYALARRAWPYRD
                     LPREDFDEIVEMLATGFTTRRGRRGAWLHHDAVNGRLRARQGARLAAVTSGGAIPDTA
                     DYQVVLEPSGAVVGTLNEDFAVESLAGDIFQLGNTSWRILKIESGRVLVEDARGQPPT
                     IPFWLGEAPARTEELSAAKSSFRVPTTA"
BASE COUNT          768 a         1645 c         1931 g          721 t
ORIGIN      
        1 gaggccgcgg ccaccagcgg ggcgccggga gcgtggctca tcagccgcgt ctggccggtg
       61 gcccgctcat ggaggaagac gtcgccctcg aaagtagaga ggcccgccac ctgcccggcc
      121 acgaggttgc tcgcgaagct atggaaggcg agccaggcgc catccgcgct gagcaccggc
      181 tgccaggagg aggcgtcgcc ggtggtggtg gccgagccgc tggcgcggct gacgagggtg
      241 accacaccgg tggcccgggc gaagaggaag acgtcgtaac cgccgttggt gtcggtctgc
      301 ccggcgacga ggtcgccggc aaagctttcg aaggcgacga ccgcgccgtc cgcgctgatc
      361 actggctgcc gggagtctcc agcggcgacc gtggtggccg atcccgggat gtggctcacc
      421 agggtcgtgg tgccggcggc gatctgatgg aggaagacat cttcgctgta gctcgggtcg
      481 gtctgcccgg ccaacaggtc cgaggcccag ctcgtgaaga cgatgaacgc gccatccgcg
      541 ctgagcctcc cataggttga ggggccgttg gcggtggtgg cgggcgcgcc ggcggcgtgg
      601 ctgatcaggg tgcgggtgcc ggcagcacgg tcgtagagga acaggtccga catgccattc
      661 ccgtccgtcg ccccgagatc ggtggccagg ctgcggaaca ggatccggga gccgtccgcg
      721 ctgatggacg ggaggcccga gtggttgttg gcgctcgtcg aggggaggcc ggcggcgtgg
      781 ctgagcaggg tgatgatgcc ggtgtcgcgg tcgtacagga agacgttgaa atagtccgtc
      841 caggactggg cctggtccgc caccagatcc tcggccaggc tctggaaggc gacccagcgg
      901 ccgtcggcgc tgatcaccgg gttggaggat gaattcgagg cggcggtcgc cggagtggct
      961 ccggccttgc ggctgaccag gagggtcgtg ccggtggcgc ggtcatggag gaagacgtcc
     1021 ctggtggcgc cgggagcggc ctcgtcctcc acctggccgg gcaccaggtt gggcgcggag
     1081 ctggtgaaga ccacccagcg gccgtcggtg ctggtgagcg tcggcagatc cgtggagtcc
     1141 gtgaaggcgc ccgcgctgag ggaaggggag gcgagccgtg agacgagctc ggcggtgccc
     1201 gcaacacggt cgtgggtata gacgtcctcg atgccgttga agtcgcggac gccgctcgcg
     1261 aggttcgcca cggtgctgac gaagacgatg tgctcgccgc cgtcgctgat ccctgacccc
     1321 accgagtttc cctgcgggta ggcggcggcc gtgcccgcga cgcggctggc cagggaggtc
     1381 cccccgccgg cgtggtcata gaggaagacg tcgtcgtcca ggttcgtctc gggagccccg
     1441 gggaggatat tgcgcgcgct ggtggtaaag gcgacgaagc ggccgtcgcc gctcaggaag
     1501 gcgcgaaagt ctccctggtc ggcgacggcg gccggtgagc ccgcggcatg gttcaccagg
     1561 gtattggcgc cggtggcccg gtcgaagagg aagatcttgg tcaggcccgc agggccgctc
     1621 tggccgggca ccaggttggt ggagagggcg ctgaagagca cccgaccccc gtcggcgctg
     1681 atccctgcga ggaaggagaa gacgccggcc tcggtgctgg ccgacgaggc gtcgtgggtc
     1741 accagggtca tggcaccggt ttgccgctcc cacaggaaga tatcctgcga gctggagccg
     1801 gtgacgccgg cgaccaggtt cgaggcggtg ctctggaagg cgacccagag tccgtcggcg
     1861 ctgatcctgg ggctggtcga ggcgccgtca cctccctggg tcatggagcc gctgctgcgg
     1921 ctgaccagga tgttggtgcc ggtcgtgcgg tcgaagagaa agacggcggt ggcgggcagg
     1981 tccgagccgg cgacgagatc ctcggagccg ctttcataga ccacccaccg gccatcggcg
     2041 ctcaaggtcg ggcggtcggc ccctgccgcg ctggcaaagg tggcagagcc cgccgcgtgg
     2101 ctgaccagac ggttggtgcc cgccgcgcgg tcataaagaa acacctgaac accggcgccg
     2161 ccgccctggc cggccaccag gttgccggct gggctggtga aggcgatgta ggcgccgtcg
     2221 gcgctgaggg agactgcgcc gccggcagtg tggttgccgg tggtggccgg cgaagaggcg
     2281 gcgtgggaga ccagagtgac cgtgcccgtc acccggtcga agaggaagag gtccgccaag
     2341 aagttgttgt cggtctggcc ggccacgagg tcggtggcga ggctcgagaa cgcgacgtag
     2401 cggccgtcgg cgctcaacac gggtgcggtg gaggagtcat tcgccgccgt cgtgggcgat
     2461 gtggcggagc ggctgaccag cacggtcgtc ccggtggccc ggtcgcgcag gaagacgtcg
     2521 gtggtgcggt tggtgtcgac ctgtcccggg acgaggtgag cctcggcgct caggaagacg
     2581 acaaagcgac cgtcggagct tggtctgccc gcgccgaagc cgatcccggc gtcattgggc
     2641 agcgtcggct cggcgcggga gacgagggag acctggctcg ccgcggcggg ggcggcggcc
     2701 aggacgagac aggccagaca ggcgaaccat ccgcccaccc tcacgggacc cgaaattcca
     2761 cacatgatga cctcctggac gatgcggacc cgccggtgag cgtgggaagc agccctattc
     2821 taccctgagg agggtgatcg cccaggagga ggtgtcggct ccggccgttc cgtttctgat
     2881 cctgcggttt acaaggtgga gggagatccc ccctcccgcg gggaggaggg ggaggacggg
     2941 aggggacggc ctagttctcg ccctcgcacg gcggcggcgg gacgcagagg aggtcgatgc
     3001 agcagtcgcc gcagcactgc tggtaacagc agacgtagcc gcaggcgttg ggagagctgc
     3061 agtaggtgac acagttgttg tagtcgcagc gcccggccgt ggcggcgggc ggggtcacga
     3121 caccggcgac ggcgaagacg agggccaggg acagcagaac cagcttgcgc atgttcagac
     3181 ctccagcaaa aggttgagga ctccattata ggccgggtgc ggggaagctt ttccttcctt
     3241 tctctgttag agtctacggg gcattttcgc ctttatcgcg cgccatggca ctgtccgact
     3301 tccatcccgc cgtctcccgc tggttcgaga agagcctggg gagcccgacc gcgccgcagg
     3361 cgcaggcctg gcccgagatc cgggcgcgcc gccacaccct gatcgccgcg ccgaccggct
     3421 cgggcaagac gctggccgcc ttcctcgccg ccatcgacga cctcgtgcgc caggccctgg
     3481 tgggggagga tggcctgccc gacgagaccg agatcgtcta cgtctcgccg ctcaaggcgc
     3541 tttcgaacga catccagaag aacctgcagg aacccttgga ggggatccgc cgcgagctgg
     3601 ccgccgctgg cctgcccgac gtcgagatcc gcaccctggt gcgcaccggc gacaccccgg
     3661 cgtccgagcg ggcggcgatg ctcaagcggc cgccccacat cctggtgacc accccggagt
     3721 cgctctacat cctcctcacc agcgaggggg ggcgcggcat cttgaagacc acccgcacgg
     3781 tgatcgtcga cgagatccac gccgtggcgg acgacaagcg gggctcccac ctctccctct
     3841 ccctggagcg gctcgaggcc ctatgtaata aggaagggcg cagcctggtg cgggtcggcc
     3901 tgtcggccac ccagaagccg atcgaggagg tggcccggtt cctggtgggg accgcgaacg
     3961 tcgaggcgga cggcacgccg cggtgcgcca tcgtcgacgc cggccatggg cggcggctcg
     4021 atctctgcct cgaggtcccc ctgtcccctc tggaagccgt gatgcccaac gaggtctggg
     4081 acgaggtcta cgaccgcctc gccggcctca tccgccagca cgagacgacg ctggtcttca
     4141 ccaacacccg gcggacggcg gagcgggtga cgcgccacct ctccgagcgg ctggggccgg
     4201 ggaaggtcac ctcccaccac ggcagcctgg ccaaggagca gcggctggag tccgagcgcc
     4261 ggctcaaggc cggggagctg tccgccctgg tggccacggc gtcgctggag ctggggatcg
     4321 acatcggctc ggtggacctg gtctgccagc tcggctcgac gcgctccatc gccacgctcc
     4381 tccagcgggt cggccgctcc ggccaccatc tgggagggct gcccaagggg cggatcttcc
     4441 cgctcacccg cgacgagctg gtggagtgca cggcgctcct cgcggcggtg cggcgcggcg
     4501 agctcgaccg gctggtgatc cccgagcgcc ccacggacgt gctggcgcag cagatcgtcg
     4561 ccgccgtggc cgccgaggag tgggaggagg acgacctcta cgcgctggcg cggcgggcct
     4621 ggccctaccg cgacctgccg cgcgaggact tcgacgagat tgtggagatg ctcgccaccg
     4681 gcttcaccac ccgccgtggg cggcgcggcg cctggctcca ccacgacgcg gtcaacggcc
     4741 ggctgcgggc gcgccagggg gcgcggctgg cggcggtgac ctccggcggc gccatccccg
     4801 acacggccga ctaccaggtg gtgctggagc cctcgggggc cgtggtcggc accctgaacg
     4861 aggacttcgc ggtggagagc ctggcgggcg acatcttcca gctcggcaac acctcgtggc
     4921 ggatcctcaa gatcgagtcg ggccgcgtgc tggtggagga cgccaggggg cagccgccga
     4981 ccatcccctt ctggctcggc gaggcgccgg cgcggaccga ggagctgtcc gccgcgaagt
     5041 cctcgttcag ggtgccgacc acggc
//
LOCUS       DASPJI010000408         9867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_22904, whole genome shotgun sequence.
ACCESSION   DASPJI010000408 DASPJI010000000
VERSION     DASPJI010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9867)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9867
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22904"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4644
                     /locus_tag="VEL74_10980"
     CDS             <1..4644
                     /locus_tag="VEL74_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG63095.1"
                     /translation="ALPDTGAAAALCLDRDAAAVAAESPERPGLPVDPGALAYAIYTS
                     GSTGRPKGVAIEHRSAAVLCHWARETFPAADLAGMLASTSINFDLSVFEIFVPLAWGG
                     TVILAENALALPRLPAAGRVTFVNTVPSAMAELVRGEGLPPSVRAAGLAGEPLKGVLA
                     ERIYSGGGVRAVYNLYGPSEDTTYSTGVRVGPGDPEEPTIGRPIANTELYLLGPDLEP
                     AAAGEPGEVHLGGEGLARGYLGRPELTAERFLPDPFSGRPGARLYRVGDLGSFRPDGE
                     VRFLGRVDHQVKIRGFRIELGEIEAALSRHPAVEEAVVMARPAPGEEGEGERRLVAYL
                     EPRPGTEIDVQELRAVLRQRLPAHMVPSLFVVLAPLPRTPNGKVDRKALPEPDWSAGA
                     GHEEASAPRTPLEDLLAGLWGDVLGLPRVGLHDPFLELGGHSLLAARLLTRVREAVGV
                     QLDLPTLFAHPTVAAFAGAVERARGASVSGGAPELPPLVPVPRAGDLPASLLQTRMWF
                     LDLIHPGSPVNNVAVAAHLAGPLRPEVLELGLREIVRRHEALRTTFAERQGTLLQRIA
                     PRLEPDLPVLDLSALPAAQREAETGRLGAARAAAPFDLRRGPVLRAELLRLGPEEHRL
                     LLGMHHIVSDDWSVWVLFRELGALYRAALGGAPHPLPAPPRIQYADFAEWQNRWLEAP
                     EALDGFTAYARRLLADVPGTLALPADRPRPPLQTFRGRHHHFALPATLPAALRGLARR
                     EGATLFMALLAGFEALVAQLTGQERFLLGSPTASRGRSELEGLIGYFANTAVWRADLT
                     GSPGFRGLLARVRDAVLEASRFEATPFERVPAELRPARQTGDHQFLQAVFVLQTVPRP
                     EPELAPGLRLRTWEMDTGTSKVDLSLFLWDDAGLTGAIEYSTDLFDESTIRRLVGYYQ
                     QVLEEALAAPDRPVGEIGRLAAAERRQVLSQQAGETAADETAPRGVVEELIAGIWSRV
                     LGVDTVGRIGRHQSFFALGGHSMLASRVYMRLRDLLPVELPFRALFEHPTVAGLALAV
                     EGARQASPLSPIQPGERSDRPPLSFDQQRLWFVERLDPGTAVLHLPALYALRGPLQVP
                     LLGEALAGIVGRHEALRTTIATADEVPFQVVHPPAAPALPLVDLSALPADRRDGEAGR
                     AADVAARHPFDLERGPLVRFLLLRAAEEDHRLLALFHHLVADGASMALFEQELARLYD
                     ALAAGRPSPLATLPVQYADYAAWQRRWLTGDRLAPQVAFWRERLAAPLPALRLPTDRP
                     RPEVPDPRGALARAVLPKELAAALRQLSLREGATLYMTLLAGFAALLHRATGQRDLAL
                     GTPVSLRDRAELEELIGLFVNTLVIRAEVSPEMGFRDLLAHVRERSLEAFAYKEVPFE
                     RLVEELRPERGAHDTPFFQVMFALQSSPETARRPGGLDPRPLDLTTGTAQFELTLYMV
                     EEGPDLVALAEYRTELFGATTIDAMLAAYGALLDAAVADPGRPVADLPLAGWPPAWVA
                     GVAGAAAPEPPKPAPPPESDAAREARLAEARSQLSAQQRDLLRQRLQRSRRTV"
     gene            complement(4758..5174)
                     /locus_tag="VEL74_10985"
     CDS             complement(4758..5174)
                     /locus_tag="VEL74_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYG63096.1"
                     /translation="MILLDTNVLIYASTAGSPFLEWARHTIAEGVSGAGAAVNAVSLA
                     EICVGDAEPETVADRLRSWGITILDVPAAVAEPCAKAYSEYRERRRVESGRDAPSLPL
                     PDFFIGAHAQVMSWPLATADEGRFRTYFPSVTLRTP"
     gene            complement(5471..6007)
                     /locus_tag="VEL74_10990"
     CDS             complement(5471..6007)
                     /locus_tag="VEL74_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63097.1"
                     /translation="MRNSEEGNTPRPSSRFNAFSESFLQLDLEANEPPRGDDPEGEGP
                     WVILPTSSQEWPDWPEPHALWPLGERPERHDPPSYVCSQRATALLTSIVRPIVGREPF
                     YELGAERWRGGFPLLRHGRPEAWMGLFHPELAAGVSLLEAVTRNPSDMALLLEAIGPS
                     ARRRVGSILLERLYGVQG"
     gene            complement(6877..9276)
                     /locus_tag="VEL74_10995"
     CDS             complement(6877..9276)
                     /locus_tag="VEL74_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG63098.1"
                     /translation="MGKTTLCAEVAPVLAETLSPGVRVLALDGRYAATQAGPVVALWQ
                     EVQAARTDEGWSQVLAGLQEKGLTAEALARALWALAQMEAGLLVYLDDAESLQVPVGE
                     GELGRWRDPGFAEFWQYLVLVAEEGGPFGVLASSRYQPEGTPSRAVLPLPEMSRYEVV
                     RLLAWMPTLGRMPAADRAWVAERVEGHPRTVEYLEALARAKEECLGLPRGHYEGENWR
                     QDILEPILPETKEKVGTDLLLGKLWDALPPAAQEHLGRCSVLTQPVPLEGIKNLEPKE
                     GTCKRLLELGLFSPFLGPTGNEWWAPHRLVTEEAQQRWKGEAREAHLMVAEWLEKQFE
                     AESNQYWAERTVEHFLAAGKADRAWPTTRLLILHLRRTGRYREALEWVERALAVGPTG
                     AILGLALTFQAQLQRMAGVSATGAEVSLLHSLDLVEQEDKPFVLNELGKLYRHQGQIQ
                     KAADYHTRSVEAEIAIKGEVHPDVAAPLHSLAGVLKEQGDLVGARQRLERSLEIKAQV
                     FGTELHPDVAISMHELAGVLKEQGDLAGARQRLERSLEIQAQIFGTEAHPSVAASLHE
                     LAGVLEAQGDLPGARQRLERSLEIQAQVFGTEAHPSVAASLHELAGVLQAQGDLAGAR
                     QRLERSREIQAQVFGTEAHPSVATSLHSLAGVLQAQGDLAGAREKLERSLAIKVQVFG
                     TEVHPSVAASLHELAGVLQAQGDLAGARQRLERSLEIQAQVYGTREHYLMAITEGNLG
                     FLLLHQGEVQQGVELLVHAFTVFQGQLGSDHPYTRRLTQFFAQFAPQSPETPPPDTAA
                     G"
BASE COUNT         1351 a         3709 c         3361 g         1446 t
ORIGIN      
        1 gccttgccgg acaccggcgc ggcggcggcc ctctgcctcg accgcgacgc ggcggccgtc
       61 gccgccgaga gcccggagcg gcccggcctg ccggtggacc ccggcgccct cgcctacgcg
      121 atctacacct cgggctccac cgggcggccg aagggggtcg ccatcgagca ccggagcgcc
      181 gccgtcctct gccactgggc gcgggagacc ttcccggccg cggacctggc ggggatgctc
      241 gcctccacct cgatcaactt cgacctgtcg gtgttcgaga tcttcgtccc cctcgcctgg
      301 gggggcacgg tgatcctggc cgagaacgcc ctcgccctcc cccggctccc cgcggccggc
      361 cgggtcacct tcgtcaacac cgtgccgtcg gcgatggcgg agctggtgcg gggggagggg
      421 ctccccccct cggtgcgcgc ggcgggactg gcgggcgagc cgctcaaggg ggtgttggcc
      481 gaacgcatct actccggtgg cggagtccgc gcggtctaca acctctacgg cccgtccgag
      541 gacaccacct actccaccgg cgtccgcgtc gggcccggcg acccggagga gccgaccatc
      601 ggccggccca tcgccaatac cgagctctac ctgctcggcc cggacctgga gccggccgcc
      661 gccggcgagc ccggcgaggt ccacctcggc ggcgaggggc tggcgcgcgg ctacctcggc
      721 cgcccggagc tgaccgccga gcgcttcctc cccgatccgt tcagcggccg gccgggggcg
      781 cgcctctacc gcgtcggcga cctcggcagc ttccggccgg acggcgaggt ccggttcctc
      841 ggccgcgtcg atcatcaggt caagatccgc ggcttccgca tcgagctggg ggagatcgag
      901 gccgcgctca gccgccaccc ggcggtggag gaggccgtgg tcatggcccg cccggccccc
      961 ggcgaggagg gcgaggggga gcggcgcctg gtggcctacc tggagccgcg accgggcacc
     1021 gagatcgacg tccaggaact gcgtgctgtc ctgcgccagc gcctgccggc ccacatggtg
     1081 ccgtccctgt tcgtggtcct cgctcccctg ccgcgtaccc ccaacggcaa ggtggaccgc
     1141 aaggcgctgc cggagcccga ctggagcgcc ggcgccggcc atgaggaggc atcggcgccg
     1201 cgcacgccgc tcgaggatct gctcgccggc ctctggggag acgtcctggg gctcccccgc
     1261 gtcggcctgc acgacccctt cctggagctg ggcggccact cgctgctcgc cgcccggctg
     1321 ctgacgcggg tacgcgaggc cgtcggcgtc cagctcgacc tccccaccct gttcgcccac
     1381 cccaccgtgg cggcgttcgc cggggcggtg gagcgggcgc gcggcgcctc ggtcagtggc
     1441 ggcgcgccgg agctgccgcc gctcgtcccg gtgccgcgcg cgggagacct gccggcctcg
     1501 ctgctccaga cccgcatgtg gttcctggac ctgatccacc ccggcagccc ggtcaacaac
     1561 gtggcggtgg cggcgcacct cgccgggccg ttgcggccgg aggtcctgga gctcgggctg
     1621 cgcgagatcg tccgccgcca cgaggcgctg cgcaccacct tcgccgaacg gcagggcacc
     1681 ctgctgcagc gaatcgcgcc gcggctggag ccggacctgc cggtcctcga cctctcggcc
     1741 ctgccggcgg cgcagcggga ggccgagacc ggccgcctcg gcgccgcccg cgccgccgcc
     1801 cccttcgacc tgcggcgcgg cccggtgctg cgcgccgagc tgctgcggct gggtcccgag
     1861 gagcaccggc tgctgctggg gatgcaccat atcgtgtcgg acgactggtc ggtctgggtg
     1921 ctgttccggg agctgggggc gctctaccgg gcggccctgg gtggcgcgcc ccacccgctg
     1981 ccggcgccgc cgcggatcca gtacgccgac ttcgcggagt ggcagaaccg ctggctggag
     2041 gccccggagg ccctggacgg cttcaccgcg tacgcccggc ggctgctggc ggacgtgccc
     2101 ggcaccctgg cgctgccggc cgaccggccg cgcccgccgc tccagacctt ccgcggccgg
     2161 caccaccact tcgcgctccc cgccaccctg ccggccgccc tgcgcggcct ggcgcggcgc
     2221 gagggggcga cgctgttcat ggcgctgctc gccggcttcg aggccctggt ggcccagctc
     2281 accggacagg agcgcttcct cctcggctcc cccaccgcaa gccgcggccg gagcgagctc
     2341 gaggggctga tcggctactt cgccaacacc gcggtctggc gggcggacct cacgggaagc
     2401 cccggcttcc gcggcctcct ggcccgggtg cgcgacgccg tgctggaggc gagccgcttc
     2461 gaggccaccc cgttcgagcg ggtgcccgcc gagctgcggc cggcgcggca gaccggcgac
     2521 catcagttcc tgcaggcggt cttcgtgctc cagaccgtgc cgcggccgga gcccgagctg
     2581 gccccggggc tgcgcctccg cacctgggag atggacaccg gtacgtccaa ggtggacctc
     2641 tccctcttcc tctgggacga cgcggggctc accggcgcca tcgagtacag caccgacctg
     2701 ttcgacgaga gcaccatccg ccgcctggtg ggctactacc agcaggttct ggaggaggcc
     2761 ctcgccgcgc cggaccggcc ggtgggcgag atcggccggc tcgccgccgc cgagcgccgg
     2821 caggtcctga gccagcaggc tggcgagaca gcggccgacg agaccgcgcc gcgcggcgtg
     2881 gtggaggagc tgatcgccgg catctggtcg cgggtgctcg gcgtcgacac cgtcgggcgc
     2941 atcgggcgtc accagagctt cttcgccctc ggcgggcatt ccatgctggc gtcccgcgtc
     3001 tacatgcgcc tgcgggacct cctgccggtg gagctgccgt tccgcgccct gttcgagcac
     3061 cccacggtcg ccgggctggc gctggcggtg gagggcgcgc gccaggcctc ccccctgtcc
     3121 cccattcagc caggtgagag gagcgaccgc ccgccgctct ccttcgacca gcagcgcctc
     3181 tggttcgtgg aacggctcga tcccggcacc gccgtgctcc acctgccggc gctctacgcg
     3241 ttgcgcggcc cgctccaggt ccctctcctg ggtgaggccc tggccgggat cgtcggccgg
     3301 cacgaggcgc tgcgcaccac catcgccacg gcggacgagg tccccttcca ggtggtccat
     3361 ccccccgccg cgccggccct gcctctggtg gacctgtccg cgttgccggc ggaccgccgc
     3421 gacggcgagg ccggacgggc cgcggacgtc gccgcccgcc atccgttcga cctggagcgg
     3481 gggccgctcg tccgcttcct gctgctgcgc gccgcggagg aggaccaccg gctgctggcg
     3541 ctcttccatc acctggtggc ggacggcgcc tcgatggccc tcttcgagca ggagctggcg
     3601 cgcctctacg acgccctggc cgccgggcgt ccgtcgcctc ttgccacgct gcccgtgcag
     3661 tacgccgact acgcggcctg gcagcggcgg tggctcaccg gggaccgcct ggcgccacag
     3721 gtggcgttct ggcgcgagcg gctggccgcc ccgctgccgg cgctgcgtct gcccacggac
     3781 cggccgcggc ccgaggtgcc ggacccgcgc ggcgccctgg cccgcgccgt cctccccaag
     3841 gagctggcgg cggcgctccg gcagctctcc ctgcgcgagg gggccaccct ctacatgacg
     3901 ctgctcgcgg ggttcgccgc tctgctccac cgcgccaccg gacagcgcga cctggcgctg
     3961 ggtacgccgg tgtcgctacg cgaccgcgcg gagctggagg agctgatcgg cctgttcgtg
     4021 aacacgctgg tgatccgcgc cgaggtgagc ccggagatgg gcttccggga cctcctggcg
     4081 cacgtccgcg agcgctcgct cgaggccttc gcctacaagg aggtcccctt cgagcgcctg
     4141 gtcgaggagc tgcggccgga gcgcggcgcg cacgacaccc ccttcttcca ggtcatgttc
     4201 gcgctccagt cgtcgcccga gacggcgcgg cggccgggcg ggctggatcc gcggccgctc
     4261 gacctgacca ccggcacggc gcagttcgag ctgacgctct acatggtcga ggaaggcccc
     4321 gatctggtgg ccctggccga gtaccggacg gagctgttcg gcgccaccac catcgacgcg
     4381 atgctcgccg cctacggagc gctgctggac gccgcggtgg ccgatcccgg ccgccccgtc
     4441 gccgacctcc ccctcgccgg ctggcccccc gcatgggtcg ccggggtcgc cggggccgcc
     4501 gcgccggagc ccccgaagcc ggcgcccccg ccggagtccg acgccgcccg cgaagcccgc
     4561 ctcgccgagg cacgctcgca gctcagcgcc cagcagcggg acctcctgcg ccagcgcctg
     4621 cagcggtccc gcaggacggt ctgacggggt cgcggaaccc atggaaatgg ccttccggcg
     4681 gatcgtctca tcgggcgggg agggcggccg tcagcccccg gggggtgcct tgggaccggt
     4741 gggaggcccg ttccccgtca aggcgtccgc aaggtgaccg acgggaagta ggtccggaac
     4801 cggccctcgt cggctgtcgc gagtggccag ctcatgacct gggcatgggc gccgatgaag
     4861 aaatcgggca gcggcagcga aggcgcgtct cgtccggact ccacccggcg gcgctcccga
     4921 tactccgagt acgccttggc gcaaggttcc gccacggcgg cggggacgtc caggatcgtg
     4981 atgccccaac tccgaaggcg gtcggcaacg gtctccggct ccgcatcgcc cacgcagatc
     5041 tccgccagac tgaccgcatt gaccgccgct cccgctcccg atactccttc ggcaatcgtg
     5101 tgccgcgccc actcgaggaa aggcgacccg gcggtggagg cgtagatcag gacgttcgtg
     5161 tcgaggagaa tcatggctcc cgggtcagag ctttcaagga gtcccaggtc attttgggct
     5221 gcaggggggc cgcagcaatg gcttccaggc agcgatccat gctcatcccg gctcccagct
     5281 ccggacgttc caggcgtacc aggagcaggc gtccctcacc ctggctctgg atgtcgaaga
     5341 cgtcccccgg tcttgccgcg ggaagcacca ctcgccgttt tgcatcagcc gtcgaaatcg
     5401 ccatggtggg attatcccac catggcctga gccagttcaa cggcccccta cctgatcacg
     5461 acgccgcccc ctacccctgt accccgtaca gccgttccag gaggatggag cccacccgcc
     5521 gccgggcgga ggggccgatg gcctccagga ggagcgccat gtcggacgga ttgcgcgtca
     5581 ccgcctccag cagactcacc cccgccgcca gctccggatg aaagagcccc atccacgcct
     5641 cgggccggcc gtgccgcagc aatgggaagc cgccgcgcca gcgctccgct cccagctcgt
     5701 agaacggctc gcgtcccaca atcgggcgga cgatggaggt caggagcgcc gtcgcccgct
     5761 gcgagcagac gtacgacggc ggatcgtgcc gctccggccg ctcgccgaga ggccacagcg
     5821 cgtgcggctc cggccagtcc ggccactcct gggaagacgt gggaaggatg acccagggac
     5881 cctcgccctc ggggtcgtct ccccgggggg gctcgttggc ctccagatcg agctgcagga
     5941 agctctcgga gaaagcgttg aaacggctgg acggacgggg ggtgttaccc tcttcagagt
     6001 tgcgcatggg attaacgctc ctttgcgtga caggctcggg taaggtctga tcacctcacc
     6061 cgggccgctt ggttgtgatg ctgcacaccg gccggcacca tgccggccgc ttcgctttgt
     6121 ggcgccgggt ccgcgccact cgccttctct ctctccccgc gatcaccccc tgtccggccg
     6181 tgaacttgtg cttccaggcg gtactctatg acgggcaagt tgggaggtca agggggaaaa
     6241 tgaaagacgc cgaggaccgg caaagctcga ccgatcgacg gccaagccga gcccgtcacc
     6301 ggcacaccgc tccccgtgac aggcaccgcg cggccgacga caggccgcac cgtgccgatc
     6361 gcaggccgcg ccatgcccgc gacaggccga gcagtgcccg cgacaggcca cgccgtgccg
     6421 atgaaaggca gcgccgtgcc ccggacgggc gccacagagc ccttcgtcgg cgcggctgtc
     6481 ccgagaatcg gcacagcatg tccagcaacg gcgacggccc gccccggggt tcccgaggac
     6541 gcctgggaag ggggcttcct ccccccctgg gcgggtgcgt gggagggagc gggggaggac
     6601 cgaggagggg ggtttcaacc gcttgccaag acccggacgc cagatctcgg caccatcgct
     6661 ccccagccgc gaagcggttc aggcctacag tttggcattt caatgccagg tacgcgcctc
     6721 ccccccaccc tccaagccct gagagggcgc agggaccgcc ccggaccgcg aactgcctgc
     6781 gtcccttcgg gactttagat tcaatggttt gtatctacct gggattgaaa tcccaggctc
     6841 tatgcctgcg ccgcttcgcg tctggatggg gcgcctctac cccgctgccg tgtctggcgg
     6901 aggcgtttcg ggggactggg gtgcgaactg ggcgaagaac tgggtcaacc ggcgggtata
     6961 gggatgatcg gagccgagtt ggccttgaaa gacagtgaag gcgtggacca gcagttccac
     7021 gccctgctga acctcgccct gatggaggag caggaagccc aagttgccct ctgtgatagc
     7081 catcaagtag tgctcgcggg tgccgtagac ctgcgcctgg atctccagag acctctccag
     7141 cctctgccgg gcacccgcca gatctccctg cgcctggagc actccggcca attcgtgcag
     7201 cgacgccgcc acggacgggt ggacctccgt gccaaagacc tgcaccttga tcgccagaga
     7261 cctctccaac ttctcgcggg cccccgctag atctccctgc gcctgaagaa ctccggccaa
     7321 cgagtgcagc gacgtcgcca cagaggggtg agcctccgtg ccaaagacct gcgcctggat
     7381 ctcccgagac ctctctagcc tttggcgggc acccgccaga tctccctgcg cctggagcac
     7441 tccggccaac tcgtgcagcg acgccgccac ggacgggtga gcctccgtgc caaagacctg
     7501 tgcctgaatc tccagagatc tctccagcct ctgccgggct cccggcagat ctccctgcgc
     7561 ctcgagcact ccggccaact cgtgcagcga cgccgccacg gacggatgag cctccgtgcc
     7621 aaagatctgc gcctggattt ccagagatct ctccagcctc tggcgggccc ccgccagatc
     7681 tccctgctcc ttcagcactc cggccagttc gtgcatcgat atcgctacgt cggggtgaag
     7741 ctccgtcccg aacacctgcg ccttgatctc cagagacctc tccagcctct ggcgggcccc
     7801 caccagatct ccctgctcct tcagcactcc ggccaacgag tgcagcggcg ccgccacgtc
     7861 tgggtgaacc tcacccttga tggcaatctc ggcttccacg gatctcgtgt ggtaatcggc
     7921 agctttctgg atctgcccct ggtgccgata tagttttcct agctcattga gaacgaaggg
     7981 cttatcctcc tgctcaacca agtccaagga atggagcagc gaaacttcgg cgcctgtggc
     8041 tgagactcca gccattctct gcaactgggc ctgaaaagtc agcgccagtc ctaggatcgc
     8101 accagtcggg ccaaccgcga gagcccgctc cacccactcg agcgcctccc ggtagcgacc
     8161 ggttcgccga aggtgcagga tcaaaagacg ggttgtaggc caagcacggt ctgcctttcc
     8221 cgccgcgagg aagtgctcaa ccgtccgttc ggcccaatac tggttgctct ccgcctcgaa
     8281 ctgcttttcg agccactcgg cgaccatcag gtgggcctcg cgagcctccc ccttccaccg
     8341 ctgctgggct tcctccgtga ctaggcggtg cggagcccac cattcattgc cggtcggccc
     8401 taggaagggg ctgaacagcc ccaattccag cagacgcttg caggtcccct ccttgggctc
     8461 aaggttctta atcccctcca gaggcaccgg ctgggtcagc acggagcagc gccccaggtg
     8521 ctcctgggcg gcgggcggga gcgcatccca gagcttcccc aggaggaggt cggtcccgac
     8581 cttctccttc gtctccggga ggatgggctc caggatatcc tgccgccaat tctctccctc
     8641 gtagtgccct cgcggcaggc cgaggcactc ttccttcgct cgtgcgagtg cctctaggta
     8701 ctccaccgtt ctgggatgcc cctccacccg ctccgccacc caggccctgt cggcggcggg
     8761 catccgcccc aaggtgggca tccaggcgag gaggcggacg acttcgtagc ggctcatctc
     8821 cggaagaggc aagacggctc tggagggcgt cccctcaggc tgataccgcg acgaggcaag
     8881 gactccgaag ggtcctccct cctccgccac caggaccagg tactgccaaa actcagcaaa
     8941 accgggatcg cgccagcgcc ccaactcgcc ctccccgacc gggacctgga ggctttcggc
     9001 gtcatccagg tagacgagca ggcccgcctc catctgggcg agggcccata gtgcccgggc
     9061 cagcgcctcg gcggtgaggc ctttctcctg aaggccggcc agcacctggc tccatccctc
     9121 atccgtacgc gccgcctgga cctcctgcca gagggcaacg acggggcccg cctgagttgc
     9181 cgcgtagcgg ccatcgaggg cgaggacccg gactcccgga ctcaacgtct cggccaggac
     9241 cggcgccacc tcggcgcaga gggtggtctt tcccaaaccg cccagaccct gcacgaccag
     9301 caggcgctgc ccccgcttcc acctgcggat cgcctccgcc ctctcgcgcc gccggcccac
     9361 gaagccgaag cggaggcgct ggacgccgac gatcccgtgc tcgacccgcg ggctgccgcc
     9421 ggcgtggtcc agacgctcga agatccgctt cctctcttcc tggggcaggt ccacggtggc
     9481 gccgcccgat gccgggggta gggtggtggc atgacccggg ccccgatgat agagggcgag
     9541 ctgcgcccag cccatcgggt agacgtgggt ggcacgccca ccgtcctcga gcggctcgga
     9601 ggaaatccgg cgcgcctggc gtagagcctc ccaggcggtg cgcccgcggc ccagggcggt
     9661 gtagaaggcg gcggcggcgc gggtggcctg tcggtcttgg accgggccga agtaggcgac
     9721 cacctggggg aagccggcgc gatggagaga ggcggcggtg gagggggcgg tctggtccac
     9781 ggcggcggtg tccaccatcc gctctcccga atgggccggg ggggctgccg gctccgcggt
     9841 ggtgccatga caggcggaaa ggtagat
//
LOCUS       DASPJI010000409        13357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_595008, whole genome shotgun sequence.
ACCESSION   DASPJI010000409 DASPJI010000000
VERSION     DASPJI010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13357)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13357
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595008"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VEL74_11000"
     CDS             complement(<1..707)
                     /locus_tag="VEL74_11000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG63099.1"
                     /translation="MPDRPTIAVDVPKVVVDLRALVPEPTGIGVYTRSLLLELAGRDG
                     GLHYLGMAHRQPRGAEELTAAGIALEHQPAPLGVLWQQLTLPRRLARGDADLLWSPLI
                     TLPLRCPLPAVATIHDLTTLLLPEAHRLKVRWSILPFLRPSLEQARRLVTISKATADD
                     IAFHFPQCAGKVRVVYPGIDPELQPGTPDEIEATRRELGAPHGYLLYVGTLEPRKNVG
                     AILDAWAGLRDADPATPP"
     gene            complement(720..2603)
                     /locus_tag="VEL74_11005"
     CDS             complement(720..2603)
                     /locus_tag="VEL74_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63100.1"
                     /translation="MEERASTTADPASDRLAHAVALLGYLGLSLLYLRPIWRLFGSHI
                     PPNPGDPVFNLWVLKWGAHQFRLGLPDFWNAPIFFPAPATITYSDHLLGPAAFFASFT
                     SLFPNPLAAFNTLFLGSFVLCGWTTWWVFRRSGLSRTAAFLGGCVFAFSSFRWDQMSH
                     IQVLLAQWIPLALWSWDRLLAEATWKRAGLFVLFYVLHVTGGSYLAYMIHYPMLAILV
                     CRLPQIRIEPRRILRVAAPAAGVAAAVFVLLNIPYLQSARKLGLERNPLEIRLNSATT
                     VSYVTPSASNFYTDEWFESWRRNENSLFPGLLATGLLLTGAAQGWRRRRQAPLRPLSP
                     VRKAALIALVLLALLGWAAGDVWTWTGTSPLGRLGLGSSEKAATWLLAGGLLALLLRR
                     RWGGNWPLRLSDMDPWHRGLLLSAVFCVLLTFPVFYLPLAKLIPGLSGMRVTARFYAF
                     ASLSIAFFAGRTLDALLARMGRRAKAAFALLAAAVLLIEVFPKPLTFHPLPQERDFPE
                     VYHWLAGRDDVRAFLELPLRDDLTAISYMYFGTLHWKPMINGYSGYLPADYYWLRENC
                     CWPAPGGSTLERLRSLGMSHIVVHEDKLGKWKRDALEEWEREEAVRLVHRSGDARVYQ
                     VLQ"
     gene            2661..4544
                     /locus_tag="VEL74_11010"
     CDS             2661..4544
                     /locus_tag="VEL74_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63101.1"
                     /translation="MPNRVLSAVCLALLLLGLSWSLYKAHVPPGPLGEEATHVLLTES
                     LWHDRDLTYDHRDLLRSYRIWNRGPKGLTLISMDGGRSMHFGEPFVYALASLPFYALL
                     GVQGLLVFNMGLFLLCLAAWWFYREGAWGGGPVGPAQATGSVGDREGGAHTKEPSRAD
                     PGLLPTRGLLLAASFFASAAFAYVFRLEPAVFTMACLFLPLVLWQALRRRSAWSTRDH
                     LLLAGAGLLLGAAFLAAGPAALLGLPILIDMALQGRWKALAAILIPALLAVSLASAAQ
                     RRATGTWSPYQAVQRSTFTDEFPVESARDLWQLRLGAQPGPWSAGPGQDASPRLLGRN
                     AWYFLAGRHTGLLPYFPFALLALALYLAGPRDRSRHLLLGAVAAYCLLILLLHPLDWH
                     GGPDALGNRSFALVYPALLLLPRWDAWSRTSRRALILLPFAAAGIWTLPAVAMPIPSL
                     AHEATPQNHVRVPAFQALPLELTLLAGNSIAGWSARTWGQAIWIVPRHNFYVDEPHPN
                     GVWVRGASSSDVLMVSPVQVDRVRLVAFSLSPENLLTLETNEEEVLVFFDSEPKRQGT
                     RIELALSPVARDLGFFASSPQDFVYRVAVTTSDGLVPARRDPQSPDTRYLGTFLDFTG
                     GGL"
     gene            4582..4998
                     /locus_tag="VEL74_11015"
     CDS             4582..4998
                     /locus_tag="VEL74_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63102.1"
                     /translation="MSEPPQPLVHEVLQQIRSGVRQRQAQVATVTAGTEEARHKLVEL
                     TSKEYIQEPMALSPRPVLGRLLVFSRKAMFHLFMKWFMRPVLEQQNGFNQTASRLLQD
                     LVQSQEALAKQVRDLSSKVDSLERRLREDSADARDR"
     gene            5000..6046
                     /locus_tag="VEL74_11020"
     CDS             5000..6046
                     /locus_tag="VEL74_11020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYG63103.1"
                     /translation="MSKRIVITINRVPFAYGGAEMLVESLRDQLVLRGHEVDVVAVPF
                     AWSSRTQLLKSALSWRLLDLTSAGGQRIDLVIATRFPSYVVKHPNKVVWLIHQMRQVY
                     DLLGTPYSDFREDEPRDRQVIDGIRAMDRRTLSEARAVYTISGNVAERLKRFNEVEGT
                     PLYPPPRLEGRYHEGDFGDYVFTAGRLDRLKRFDLLIRALEHTQAPVRCRIAGEGPER
                     GKLEELIARLRLGDRVELLGWASDDAMVEHYAGSLAVFYAPYDEDYGYVTVEAFKSGK
                     PVVTAADSGGVLEFVEEGRNGFICPPDAPREIAARLDTLYKDRERARKMGQVGMETVA
                     GIGWDAVIRGLVGE"
     gene            6494..7891
                     /locus_tag="VEL74_11025"
     CDS             6494..7891
                     /locus_tag="VEL74_11025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYG63104.1"
                     /translation="MRVAYFSPLPPMRSGIADYSAELLPALAAHLDLELFVDDGFRVD
                     PALAARFPVRGHRAFPALREEGRYDAVVYQIGNNVDYHAGIYEMLLRYPGITVLHEYV
                     LHHLVRGMTLVRGDMPGYVEEMRYAYGRTGQAMARRSLDTGIPLDVWTYPLFERVVDA
                     SLGIIVHNDCTGRRVLASRPLAPVARVPHHLSLGEAEGSGPCPAELRARFGIAPDAFV
                     VGSFGFITPAKRLDVSLRAFARLRREVPDAVYLLVGETSPYYDFASLLTPELSPGVVQ
                     AGRVEIGDFLRLMQVPDVTVNLRYPSAGETSGTLIRLLGAGKPVIVSDTGAFSEIPDD
                     CCAKVDLDETEEELLLAYLRRFALDEGLRRRTGDNARRYIAAHHTLEGSARGYADVIR
                     QVVESGRPPFRPAPPLLPYPPEDLLTEMVSDVTAEAVDLGADEGDEEMMRELAGTFTE
                     LGIVPRRPAGREAGR"
     gene            7888..9225
                     /locus_tag="VEL74_11030"
     CDS             7888..9225
                     /locus_tag="VEL74_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63105.1"
                     /translation="MRRLLKPGLFLVFLALAAGHVLYWYGARERPAVPDRDGLPARLI
                     ASGAYEACLWAPYPHQNVGELGESIDDGAAWLGAASRMAGMPPPVLPSFGPFALPPSH
                     EIAACSDLDGNRYMAVARVYPALGFVARLAGRIAGNPWLAGGDASEVAGTGEQVLERR
                     VRVEWRDGLWILTSGEPPGRDEIPASAPSGAPDGDGTPILGMLSLRQEVSQFPRGDYR
                     LRRLADGDLELALDGAPDGLGQQLLALDLASVAPREESPALLAVAGPAWPADSDRPLP
                     PAAFTLFNTRDGIKVNLLEDLPGIAVLHPRGEERWTLPGQQVIRLLARGLPKGNAAGW
                     DIVAVDAASMRRARALAPRLSALVPPDATGAVGSDGRLILGLWFQPRPTQQVVGRVRR
                     VLQKIPLVDREQVRKWRDGEILLDPLTSCRQVTLAATETPASFRLRFHRCQGE"
     gene            9304..9846
                     /locus_tag="VEL74_11035"
     CDS             9304..9846
                     /locus_tag="VEL74_11035"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HYG63106.1"
                     /translation="MKIWLDQVRDEPFNWDETQTVPLEALERPELLELGPVSWRGQVV
                     SAEPGFFLRGRLSYDQTLSCNRCLKPIVESAGSDVELLILVEPQPGAAGGEHELQERD
                     LGVYYVDEEEVLETDPILLEQLQLNIPMKPLCQPDCKGLCPNCGADLNAGPCSCEETS
                     VDPRWAGLAALKSRLDGEQG"
     gene            9887..10072
                     /gene="rpmF"
                     /locus_tag="VEL74_11040"
     CDS             9887..10072
                     /gene="rpmF"
                     /locus_tag="VEL74_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009869122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HYG63107.1"
                     /translation="MPNPKTRHSKARRDKRRAHDALARPTTSTCPNCGEVKLPHRACG
                     HCGHYRGRQVVEGKDAQ"
     gene            10200..11255
                     /gene="plsX"
                     /locus_tag="VEL74_11045"
     CDS             10200..11255
                     /gene="plsX"
                     /locus_tag="VEL74_11045"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HYG63108.1"
                     /translation="MPIAVDAMGGDFAPQSAVEGSFRAAAEDGASILLVGDRARVEAE
                     LRRHGHLDGLPNGGRIEVVHAEEVVGMDEPAISPIRKKRRSSVRVCAELVKQGQAQAM
                     VTAGNTGAAMISAKMVIGTVAGVDRPALAAVLPNLKGKTVLLDVGANVNSKPLHLREF
                     AVMGHFYAQAVLGTVSPRIGLLSIGEEEGKGTDLTREVFKVLKNTGLNFVGNVEGRDV
                     FNGEVDVVVCDGFVGNVVLKSAEGVAELVGRMLREELERNPRTKSGYLLAKPAFDRFR
                     SRTDYREYGGAPLLGVNGGCLIGHGRSNARAIQNAIRRASEFVAARLDSKIRDKIAEL
                     HSQEARLLGSPQEASAG"
     gene            11252..12253
                     /gene="fabD"
                     /locus_tag="VEL74_11050"
     CDS             11252..12253
                     /gene="fabD"
                     /locus_tag="VEL74_11050"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HYG63109.1"
                     /translation="MTISPDSRIAGRTVAFVFPGQGSQKPGMGRAWAEARAEARQTFE
                     EADRVLGFPLSRLCWEGPEDELGLTVNTQPALLTTSVAIHRCLQDSPLAAVAHAGHSL
                     GEYSAFVAAGALDFADALRLVRRRGELMQEAVPVGVGGMAAIIGLDTDAVTAIAQESG
                     TAGEVCAVANLNGPGQTVLAGHKGAIDRAVALAKERGARKATVLAVSAPFHSPLMRPA
                     REGMAELLDKTTFADPRVPVVSNVDAAPVTTGAAARDALIRQIDNPVRWVESVVWMAE
                     QAGVGAFLEVGPGNVLSGLNRRIVTGVVTAGLAEPDQLRKLLEGGPEEGKEGKEGKE"
     gene            12253..13002
                     /gene="fabG"
                     /locus_tag="VEL74_11055"
     CDS             12253..13002
                     /gene="fabG"
                     /locus_tag="VEL74_11055"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HYG63110.1"
                     /translation="MFRLDGKTALVTGASQGIGEAIARRLAAQGARVVLAARNEEKLT
                     RLAGEIEGAAGSGGARPLVLDVSQPETFGERLKSLPEDYQAVDILVNNAGITADNLLA
                     RMSLEDWERVLRTNLTGAFALTKEVVRGMMRRRWGRVVNVSSVVGLMGNAGQANYAAS
                     KAGLIGFSKSLARELGSRNITVNVVAPGYIETAMTGALGDDQKSALTDSIALKRLGTV
                     DDIAWAVLYLASEEGGYVTGQALNISGGLYI"
     gene            13114..13350
                     /locus_tag="VEL74_11060"
     CDS             13114..13350
                     /locus_tag="VEL74_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HYG63111.1"
                     /translation="MGVQERVKSIIVEQLGVDEDEVSLEASFTDDLGADSLDIVELVM
                     AFEEEFGIEIPDEDAEKLQTVGDAITYIRQNQGE"
BASE COUNT         1973 a         4643 c         4754 g         1987 t
ORIGIN      
        1 ggcggtgtgg ccgggtcggc gtcgcgcagc ccggcccagg cgtcgaggat cgcgccgacg
       61 ttcttgcgcg gctcgagggt accgacgtag agcaggtagc cgtggggagc gcccagctcc
      121 cgccgcgtcg cctcgatctc gtccggcgtg cccggctgga gctccgggtc gatgccgggg
      181 tagaccaccc gcaccttgcc ggcgcactgc gggaagtgga aggcgatgtc gtcggcggtg
      241 gccttggaga tggtcaccag gcggcgcgcc tgctcgaggg agggccggag gaaagggagg
      301 atgctccagc gcaccttgag ccggtgggcc tcgggcagca gcagggtcgt caggtcgtgg
      361 atggtcgcca cggccggcag cgggcagcgc agcggcaggg tgatgagcgg cgaccagagg
      421 aggtcggcgt ccccgcgggc gagacggcgg gggagagtga gctgctgcca gaggacgccc
      481 agcggcgccg gctggtgctc cagggcgatg cccgcggccg tcagctcctc ggccccgcgc
      541 ggctgccggt gggccatgcc gaggtagtgg agaccgccgt cgcggccggc cagctccagg
      601 agaagggagc gggtatagac gccgatgccg gtgggctcgg ggacgagggc gcgcaggtcg
      661 accacgacct tggggacatc gacggcgatc gttgggcgat ccggcatggc gggcggatct
      721 tactggagga cctggtagac gcgggcgtcc ccggatcggt ggacgaggcg gaccgcctcc
      781 tcccgctccc actcttcgag ggcgtcgcgc ttccacttcc ccagcttgtc ctcgtggacc
      841 acgatgtggc tcatgcccag ggaccgcagg cgctccagag tcgagccgcc gggggccggc
      901 cagcagcagt tctcgcgcag ccagtaatag tcggccggca ggtagccgct gtagccgttg
      961 atcatcggct tccagtggag ggtgccgaag tacatatagg agatcgccgt caggtcgtcc
     1021 cgcaggggca gctctaggaa ggcgcggacg tcgtcgcggc cggccagcca gtggtagacc
     1081 tccgggaagt cccgctcctg ggggagcgga tggaaggtca ggggcttggg aaagacctcg
     1141 atgaggagga cggcggcggc aaggagcgcg aaggccgcct tcgccctcct ccccattcga
     1201 gcgaggaggg cgtccagggt gcggccggcg aagaaggcga tcgagaggga ggcaaaggcg
     1261 tagaagcggg cggtcaccct catgcccgag aggcccggga tcaacttggc gagtgggaga
     1321 tagaagaccg ggaaggtgag gaggacgcag aagacggccg agaggagtag accgcggtgc
     1381 caagggtcca tgtccgacag gcggaggggc cagttgcccc cccagcggcg gcggaggagg
     1441 agggcgagga ggcctccggc caggagccag gtggccgctt tttcggagga gcccaggccg
     1501 aggcggccca gggggctggt gccggtccag gtccagacgt ctccggccgc ccagcccagg
     1561 agggcgagga ggacgagggc gatgagggcg gctttgcgga caggggagag cgggcgcagt
     1621 ggggcttggc ggcggcggcg ccagccctgg gcggcgccgg tgaggagcag gccggtcgcc
     1681 aggaggccgg ggaagagcga gttctcgttg cggcgccagg actcgaacca ttcgtccgtg
     1741 tagaaattgc tcgccgaggg ggtgacgtag ctgacggtgg tggccgagtt gagccggatc
     1801 tccagggggt tgcgctccag gcccagcttc cgcgcgctct ggaggtaggg gatgttgagg
     1861 aggacgaaga ccgcggcggc gaccccggcg gccggggcgg ccacccgcag gatgcggcgc
     1921 ggctcgatcc ggatctgggg aaggcggcag accaggatgg ccagcatcgg ataatggatc
     1981 atgtaggcga gatagctccc gccggtcacg tgcaggacat agaagaggac gaagagcccc
     2041 gcccgcttcc aggtggcttc ggccaggagg cggtcccagg accagagggc cagagggatc
     2101 cactgggcga ggaggacctg gatgtggctc atctggtccc agcggaagga ggagaaggcg
     2161 aagacgcagc cgccgaggaa cgccgccgtc cgggagaggc cgctgcgccg gaagacccac
     2221 caggtggtcc agccgcaaag gacgaaggaa ccgaggaaca gggtgttgaa ggcggccagg
     2281 ggattgggga acaggctggt gaacgacgcg aagaacgccg ccggccccag gaggtggtcc
     2341 gagtaggtga tggtggccgg ggccgggaag aagatgggcg cgttccagaa gtccggcaac
     2401 cccagccgga actggtgggc cccccatttg agaacccaga ggttgaagac cgggtccccc
     2461 gggtttggcg ggatgtggct gccgaacagc cgccagatcg gccgcaggta gagcagggac
     2521 agcccgagat accccagcaa cgccacggcg tgcgccaggc ggtcggaggc tggatcggca
     2581 gtcgtcgagg ctctttcttc cattggggcg gggcgtattc tagaggcagg cccctggatc
     2641 ttgtatggtt cccccctgga atgccgaacc gtgtcctctc agccgtctgc ctggccctgc
     2701 tgctcctcgg actctcctgg agcctgtaca aggcccacgt ccccccgggc cccctgggag
     2761 aagaggcgac gcacgtcctc ctgaccgaga gcctctggca cgatcgcgac ctcacctacg
     2821 atcaccggga cctcctccgt tcctaccgca tctggaaccg gggtcccaag ggcctcaccc
     2881 tgatcagcat ggacggcggc cggagcatgc acttcggcga gcccttcgtc tacgccctgg
     2941 catccctccc cttctacgcc ctcctcggag tccagggcct cctcgtcttc aacatgggcc
     3001 tctttctcct ctgtctggcc gcctggtggt tctaccgaga gggggcgtgg ggaggaggcc
     3061 cggtcggccc ggcgcaggcg actggcagcg tgggggacag ggagggcgga gcgcacacga
     3121 aggagccgag cagggccgat ccgggcctcc tccccacgag ggggctcctc ctcgccgcct
     3181 ccttcttcgc ctccgccgcc ttcgcctacg tcttccggct ggagccggcg gtcttcacca
     3241 tggcctgcct cttcctgccg ctcgtcctct ggcaggccct gcgccgccgc tctgcctgga
     3301 gcacccggga ccacctcctc ctcgccggag ccggcctcct cctgggggcg gccttcctgg
     3361 cagccggccc cgccgccctg ctggggctcc ccatcctcat agacatggcc ctccaaggcc
     3421 gctggaaagc tctcgccgcc atcctgatcc ccgcgctcct ggcggtctcc ctcgcctccg
     3481 ccgcccagcg gcgcgccacc ggcacctgga gcccctacca ggcggtccag cggagcacct
     3541 tcaccgacga gttcccggtc gagagcgccc gcgacctatg gcagctccgc ctgggcgcgc
     3601 agccgggccc ctggtcggcc ggcccggggc aggacgcgag cccgcgcctc ctgggccgca
     3661 acgcctggta cttccttgcc ggccgccaca cggggctcct cccctacttc ccgttcgccc
     3721 tgctggcgct ggccctctac ctcgccggac ccagggaccg ctcccgccat ctcctcctgg
     3781 gggccgtagc ggcctactgc ctgctgatcc tgctgctcca tcccctggac tggcacggcg
     3841 gcccggacgc cctgggcaac cgctccttcg ccctggtcta tccggcgctc ctcctgctgc
     3901 cccggtggga cgcgtggagc cggacctccc ggcgcgcgct catcctcctc ccctttgccg
     3961 ccgcggggat ctggaccctg ccggcggtgg ccatgcccat cccctccctg gcgcatgagg
     4021 cgacgcccca gaaccacgtc cgcgtcccgg ccttccaggc gctccccctg gagctgaccc
     4081 tgctcgccgg caacagcatt gccggctggt cggcccggac ctggggacag gcgatctgga
     4141 tcgtgccgcg gcacaacttc tacgtcgacg agccccatcc caacggcgtc tgggtgcggg
     4201 gcgcctccag ctccgacgtg ctgatggtct cgccggtcca ggtcgaccgg gtccggctcg
     4261 tcgccttctc cctgtcgccg gagaacctcc tgaccctgga gaccaacgag gaggaggttc
     4321 tggtcttctt cgacagcgag cccaagcgcc aggggacgcg gatcgagctc gcgctctctc
     4381 ccgtcgcccg ggacctcggc ttctttgcct cctctcccca ggacttcgtc taccgcgtcg
     4441 ccgtgaccac gtccgacggg ctggtgccgg cccgccgcga tccccagagc ccggacacgc
     4501 gatatctggg caccttcctc gacttcacgg gcggcggcct gtaatctctc ccaccgtttc
     4561 cccgtttccg agagcacgac catgagtgag cccccccagc ctcttgtcca tgaggtcctg
     4621 cagcagatcc gctcgggcgt ccgccagcgc caggcccagg tggccaccgt caccgccggc
     4681 accgaggagg cgcggcacaa gctggtcgag ctgacctcca aggagtacat ccaggagccg
     4741 atggccctct ccccccggcc ggtgctcggg cgcctcctgg tgttctcgcg caaggccatg
     4801 ttccacctct tcatgaagtg gttcatgcgc cccgtcctcg agcagcagaa cggcttcaac
     4861 cagaccgcca gccggctgct ccaggatctg gtgcagtcgc aggaggcgct ggccaagcag
     4921 gtgcgggacc tctcctctaa ggtggactcg ctggagcggc ggctgcgcga ggattccgcg
     4981 gacgcgagag accgctgaca tgtcgaagcg catcgtgatc accatcaacc gcgtcccctt
     5041 cgcctacggc ggggcggaga tgctggtcga gtcgctgcgc gaccagctcg tgctgcgcgg
     5101 ccacgaggtg gacgtcgtcg ccgtgccctt cgcctggtcg agccgcacgc agctcctgaa
     5161 gagcgccctc tcctggcgcc tcctggacct cacctccgct ggggggcagc ggatcgacct
     5221 ggtgatcgcg acgcgctttc cctcgtacgt ggtgaagcat ccgaacaagg tggtctggct
     5281 gatccaccag atgcggcagg tctacgacct cctggggacg ccctacagcg acttccggga
     5341 ggacgagccg cgggaccgcc aggtcatcga cggcatccgg gccatggacc ggcggacgct
     5401 atccgaggcg cgggcggtct acaccatctc ggggaacgtg gccgagcggt tgaagcgctt
     5461 caacgaggtg gaggggacgc cgctctatcc gccgccgcgc ctggagggga gataccacga
     5521 gggggacttc ggcgactacg tcttcaccgc cggccggctc gaccgcctca agcgcttcga
     5581 cctcctgatc cgggcgctgg aacacacgca ggcgccggtg cgctgccgca tcgccggcga
     5641 ggggccggag cggggcaagc tcgaggagct gatcgcccgg ctccggctcg gcgaccgggt
     5701 ggagctcctc ggctgggcga gcgacgatgc catggtcgag cactacgccg gcagcctcgc
     5761 cgtcttctat gctccctacg acgaggacta cggctacgtg accgtcgagg cgttcaagag
     5821 cggcaaaccg gtggtgaccg ccgccgactc cggcggcgtc ctcgaattcg tcgaggaggg
     5881 gaggaacggc ttcatctgcc cgccggacgc cccgagagag atcgccgccc gcctcgacac
     5941 cctttataag gaccgggagc gcgcccgcaa gatggggcag gtggggatgg agacggtagc
     6001 cgggatcggg tgggacgcgg tgatccgggg gctggtgggg gagtaggagg gcctgagcct
     6061 tcccgcccgc cgttccccat tcatgatccc gagtgccccc gtctccgtac ccgtggagac
     6121 ggggttcatt cctcccgggg cagccgttgc tccgcgagag atcgccgtcc taccccggga
     6181 aaagttcgtt ctatccgagg caaggccatc atcccgaggg ggaagacgac cgtacccgcg
     6241 gggtgatcgc cgtacccgca gggatgacct ccgtacctcg ggtaggaagg ccgtagccgc
     6301 ggggatggtg tccggctgcc cggagacggc cttgtttcgg cgggtagggc ctttctctcc
     6361 gggtaccgtc ttcgacttcc gtggatcgcc ttcgtttttc ggagatgctt gcggtttcac
     6421 ccagctctcc caagaagcaa gcggcctatg gggcctaagg gggggacggc tgctacaatc
     6481 ccgcctcccc ctcatgcgcg tcgcctattt cagccctctg cccccgatgc gctcggggat
     6541 cgccgactac agcgccgagc tgttgccggc cttggccgcg cacctcgacc tcgagctgtt
     6601 cgtcgacgac gggttcaggg tggacccggc gctggcggcc cgcttcccgg tgcgggggca
     6661 ccgcgcgttc ccggccctcc gggaggaggg acggtacgac gccgtggtct accagatcgg
     6721 caacaacgtc gattaccacg cgggcatcta cgagatgctc ctgcgctatc ccgggatcac
     6781 cgtgctccac gagtacgtgc tccaccacct ggtgcggggc atgacgctgg tccgggggga
     6841 catgcccggc tacgtggagg agatgcgcta tgcctatggc cgcaccggtc aggccatggc
     6901 ccggcgcagc ctcgacaccg gcatccccct cgacgtctgg acctacccgc tgttcgagcg
     6961 ggtggtcgac gccagcctgg ggatcatcgt ccacaacgac tgcacgggac ggcgggtgct
     7021 ggcgagccgg cctctcgccc cggtcgcccg ggtgccccat cacctgagcc tgggggaggc
     7081 ggagggctcc gggccatgtc ccgcggagct gcgggcgcgt ttcggcatcg cgccggacgc
     7141 gttcgtcgtc ggctcgttcg gcttcatcac cccggccaag cggctggacg tcagcctccg
     7201 cgccttcgcc cggctgcgcc gcgaggtccc ggacgccgtc tacctgctgg tgggggagac
     7261 ctccccctac tacgacttcg ccagcctcct cacccccgag ctctcgccgg gggtcgtgca
     7321 ggccgggcgg gtggagatcg gcgacttcct gcggctcatg caggtgcccg acgtcaccgt
     7381 gaacctccgg tacccctcgg ccggcgagac ctccggcacc ctgatccgcc tgctaggggc
     7441 cggcaagccg gtgatcgtca gcgacaccgg cgccttctcc gagatccccg acgactgctg
     7501 cgccaaggtg gacctcgacg agaccgaaga ggagctgctc ctggcctacc tgcgccgctt
     7561 cgccctcgac gagggcctcc gccgccgcac cggcgacaac gctcgccgct acatcgccgc
     7621 ccatcacacg ctggagggct cggcgcgcgg ctacgcggac gtcatccggc aggtggtgga
     7681 gtcgggccgg ccgccgttcc ggccggcgcc gcccctcctg ccgtatccgc cggaggacct
     7741 gttgaccgag atggtgagcg acgtcacggc cgaggcggtg gacctgggcg ccgacgaggg
     7801 ggacgaagag atgatgcgcg agctcgccgg gacctttacc gagttgggaa tcgttccgcg
     7861 gcgtccggcc ggccgggagg cgggccggtg aggcgcctcc tcaagcccgg gctcttcctc
     7921 gtcttcctgg ccctcgccgc cggtcacgtc ctctattggt atggcgcgcg cgagcggccg
     7981 gcggtgccgg accgcgacgg cctgccggcc cggctgatcg cctccggcgc ctacgaggcc
     8041 tgcctctggg ccccctaccc gcaccagaac gtcggcgagt tgggtgagtc gatcgacgac
     8101 ggcgccgcct ggctcggggc cgcctcccgc atggccggga tgccgccgcc ggtccttccc
     8161 tcgttcggcc cgttcgccct gcccccctcc cacgagatcg ccgcctgttc cgacctggac
     8221 ggcaaccgct acatggccgt ggcgcgggtc tatccggccc tcggcttcgt cgcccggctg
     8281 gccggccgca tcgccggcaa cccctggctc gccggcggcg acgcctccga ggtggcgggc
     8341 accggcgagc aggtgctcga gcgccgggtg cgcgtggagt ggcgggacgg gctgtggatc
     8401 ctgacctccg gcgagcctcc cggccgcgac gagatcccgg cgtccgcgcc gtctggtgct
     8461 ccggacggcg acgggacacc gatcctgggg atgctctcgc tgcgccagga ggtctcccag
     8521 ttcccccgcg gcgactatcg cctgcggcgc ctcgcggacg gcgacctgga gctggccctc
     8581 gacggggctc cggacggcct cggccagcag ctcctcgcgc tcgacctggc ctccgtggct
     8641 cccagggagg agagcccggc gctgctggca gtggcgggcc cggcctggcc ggcggactcg
     8701 gaccggccgc tgccgccggc cgcctttacc ctgttcaaca cccgcgacgg catcaaggtc
     8761 aacctgctgg aagacctgcc cgggatcgcc gtgctccacc ctcggggaga ggagcgctgg
     8821 acgctgcccg gccagcaggt gatccgcctg ctcgcccgcg ggctgccgaa ggggaatgcc
     8881 gccggttggg acattgttgc cgtcgatgcc gcgagcatgc ggagagccag ggccctcgcc
     8941 ccgcgtctct ccgccttggt cccaccggat gcaaccggcg cggtgggatc ggatgggcgc
     9001 ctgatcctcg gcctctggtt ccagccacgg ccgacccagc aggtggtcgg ccgggtccgc
     9061 cgcgtcctcc agaagatccc tctggtcgac cgcgagcagg tccggaagtg gcgggacggg
     9121 gagattctcc tcgatccgct gacctcctgc cgccaggtga cgctcgcggc gaccgaaacc
     9181 cccgcctcgt tccgtctccg gttccaccgg tgccagggag agtagagtcg ccgtctttgg
     9241 ttgacagcgg gggcggtgat cccttagcct ccacgtttgc ccggttgaat gaggtgaatt
     9301 ggtatgaaaa tctggctcga tcaggtacgc gatgagccgt tcaactggga cgagactcag
     9361 actgtgccgt tggaggccct ggagcgtccc gagctcctgg agctgggccc cgtctcctgg
     9421 cggggtcagg tggtgtccgc cgaacccggg ttcttcctgc gggggcggct ctcctacgac
     9481 cagacgctga gctgtaaccg ttgtctgaaa ccgatcgtcg agagtgccgg ctcggacgtg
     9541 gagctcctca ttctggtgga gccccagccc ggggcggcgg gtggtgagca cgagctgcag
     9601 gagcgggacc tgggcgtcta ctatgtggac gaggaagagg ttctggagac cgatccgatc
     9661 ctgctcgagc agctccagct caacatcccg atgaagccgc tctgccagcc cgactgcaag
     9721 ggtctgtgcc ccaactgcgg ggcggacctg aacgccgggc cgtgctcgtg cgaggagacc
     9781 tcggtcgacc cgcgctgggc cggattggcg gccttgaaga gccggctcga cggcgagcag
     9841 ggctgaaaga acccgtagaa cgactcgaac aacagaggct acgaccatgc cgaatccgaa
     9901 gacccgccat tcgaaggccc gccgcgacaa gcgccgggcg cacgacgccc tcgcccggcc
     9961 gaccacctcc acctgcccca actgcggcga ggtcaagctg ccgcaccgcg cctgcggcca
    10021 ctgcggccac taccgcggcc ggcaggtcgt cgagggcaag gacgcacagt agccctggca
    10081 gagcgccctc acccccagtc ctccccgggg tgagggccga cgcgccggcg tcaggggctc
    10141 cccggcaccg tctgcgctag aatccgctgg ccaagccgaa aggttcgccg agtccgagca
    10201 tgccgatcgc ggtagatgcc atgggtgggg acttcgcgcc gcaatccgcg gtcgaggggt
    10261 ctttccgcgc cgccgccgaa gacggggcct ccatcctcct cgtgggggac cgcgcccgcg
    10321 tcgaggcgga gctgcgccgc catggccatc tcgatggctt gccgaacggc ggccgcatcg
    10381 aggtcgtcca cgccgaggag gtggtcggca tggacgagcc cgccatctcc ccgatccgca
    10441 agaagcgccg ctcctcggtc cgcgtctgcg ccgagctggt caagcaggga caggcccagg
    10501 ccatggtcac cgccggcaac accggcgccg ccatgatctc ggccaagatg gtgatcggca
    10561 ccgtcgccgg tgtcgaccgc ccggccctcg ccgccgtgct ccccaacctc aagggcaaga
    10621 ccgtgctcct cgacgtcggc gccaacgtca acagcaagcc gctccacctg cgcgagttcg
    10681 ccgtcatggg ccatttctat gcccaggcgg tgctcggcac cgtgtcgccg cgcatcggcc
    10741 tcctctccat cggcgaggag gagggcaagg gcaccgacct cacccgtgag gtcttcaagg
    10801 tgctcaagaa caccggcctc aacttcgtcg gcaacgtcga gggcagagac gtgttcaacg
    10861 gcgaggtgga cgtggtggtc tgcgacggct tcgtcggcaa cgtggtgctc aagagcgccg
    10921 agggggtggc cgagctggtg ggccgcatgc tgcgcgagga gctggaacgc aacccgcgca
    10981 ccaagagcgg ctacctgctg gccaagcccg ccttcgaccg cttccgttcg cgcaccgact
    11041 accgggagta cggcggcgcg ccgctcctcg gggtcaacgg cggctgcctg atcggccacg
    11101 gccgctcgaa cgcgcgcgcc atccagaacg ccatccgccg ggcgagcgag ttcgtcgccg
    11161 cccgcctcga cagcaagatc cgcgacaaga tcgccgagct ccacagccag gaggcgcggc
    11221 tgctcggctc tccccaggag gcttccgccg gatgaccatc tcccccgaca gcaggatcgc
    11281 aggccgcacc gtcgccttcg tctttcccgg ccagggctcc cagaaaccgg gcatgggccg
    11341 cgcctgggcc gaggcccgcg ccgaagcccg ccagacgttc gaggaggccg accgcgtcct
    11401 cggcttcccc ctctcgcgcc tctgctggga gggtccggaa gacgaattgg gtctgactgt
    11461 caacacacag cccgctctgc tcactacttc tgtggcgatc caccgttgcc tccaggattc
    11521 gccgctcgcg gccgtggccc acgccggcca cagcctcggc gagtactcgg ccttcgtcgc
    11581 cgcgggcgcc ctggacttcg ccgacgccct gcgcctggtg cgccggcgcg gcgagctgat
    11641 gcaggaggcg gtgccggtcg gcgtcggcgg catggccgcg atcatcggcc tggataccga
    11701 cgcggtgacg gcgatcgccc aggagtcggg cacggccggc gaggtctgcg ccgtggccaa
    11761 cctgaacggg ccggggcaga ccgtgctcgc cggccacaag ggggcgatcg accgcgccgt
    11821 ggccctggcc aaggagcggg gggcgcgcaa ggcgacggtc ctcgcggtgt cggcgccgtt
    11881 ccactcgccg ctgatgcgcc cggcacgcga agggatggcc gagctgctgg acaagacgac
    11941 gttcgccgat ccgcgcgtgc cggtggtgag caacgtcgac gccgcgccgg tgaccacggg
    12001 cgccgccgcg cgggacgcgc tgatccgcca gatcgacaac ccggtgcgct gggtggagtc
    12061 ggtggtctgg atggcggagc aggccggagt cggtgccttc ctggaagtcg ggccgggcaa
    12121 cgtgctgtcg gggctgaacc gtcgtatcgt caccggcgtg gtgaccgccg gcctggcgga
    12181 gcccgaccag ctccgcaagc tgctggaggg tggccccgag gaaggcaagg aaggcaagga
    12241 ggggaaggag tgatgttcag gctcgacggc aagacggccc tggtgaccgg agcttcgcag
    12301 gggatcggcg aggcgatcgc ccgccgccta gcggcgcagg gggcgcgggt ggtgctggcc
    12361 gcgcgcaacg aggagaagct gacccggctg gccggggaga tcgagggcgc cgctggctcc
    12421 ggtggtgccc ggccgctggt cctagacgtg tcgcagcccg agaccttcgg cgagcggctc
    12481 aagagcctgc ccgaggacta ccaggcggtc gacatcctgg tcaacaacgc cggcatcacc
    12541 gccgataacc tgttggcacg catgtcgctg gaggattggg agcgggtgct gcgcaccaac
    12601 ctgaccggcg ccttcgcgtt gaccaaggag gtggtccggg ggatgatgcg ccggcgctgg
    12661 ggacgggtgg tcaacgtctc gtcggtggtg ggcctgatgg gcaacgccgg ccaggcgaac
    12721 tacgccgcct ccaaggcggg cctcatcggg ttcagcaagt cgctggcccg ggagctgggg
    12781 agccgcaaca tcacggtcaa cgtggtggcc cccggctaca tcgagacggc gatgaccggc
    12841 gcgctggggg acgaccagaa gagcgcgctc accgactcga tcgcgttgaa gcggctgggc
    12901 accgtggacg acatcgcctg ggcggtgctc tacctggcca gcgaagaggg gggctacgtc
    12961 accggacagg ccctcaacat ctcgggcggc ctctacatct gaggtccgga aaccgcgcgg
    13021 agccgctgtt tcggcattgg actccgccat tgtctttgtg ttagaaccgc agttcgtgcg
    13081 cgctccgcgc gaacgcagaa caaggaggga atcatgggag tacaggagcg ggtcaagtcc
    13141 atcattgtgg agcagctcgg cgtcgatgag gacgaggtgt ccctggaggc cagcttcacc
    13201 gatgacttgg gagccgattc tctggatatc gtcgagctgg tgatggcctt cgaggaggag
    13261 ttcgggatcg agatccccga cgaggacgcc gagaagctgc agacggtcgg cgacgccatc
    13321 acctacatcc gtcagaacca gggcgagtag gcgcggc
//
LOCUS       DASPJI010000410         5036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_595017, whole genome shotgun sequence.
ACCESSION   DASPJI010000410 DASPJI010000000
VERSION     DASPJI010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595017"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..845)
                     /locus_tag="VEL74_11065"
     CDS             complement(69..845)
                     /locus_tag="VEL74_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63112.1"
                     /translation="MKRIVLACVAALSLTGCFDVENKLSLNKDLSGKAGFAMKVNMEP
                     MVLMMARMQRQMAGQEGDPTPAELAELKKSFKSTGTSPDKAKMNAEMDKFRKSLPPGV
                     KLVENTFAEKELGFDLGLGFEFDNVSKLALIKLPGKTDAQGQAAPGPENPFDSPFGGL
                     EVKDEGATILLTSKTVNPAAEAEQQSPMGEMDPEMKKQVAEMFKGLRLAYRIEAPFEV
                     VEHNATRKQGNTLIWEYNIENLEKMKPEDLAQGVRVRYKK"
     gene            complement(1009..2088)
                     /gene="prfA"
                     /locus_tag="VEL74_11070"
     CDS             complement(1009..2088)
                     /gene="prfA"
                     /locus_tag="VEL74_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HYG63113.1"
                     /translation="MLEQLSQLEKTHEELTQKLTDPTLLSDRKAYAETNRTLSEIAHV
                     VGLFREYRSVQRQIEENDELLASLGKDDDLFEMAHEERRELAERLERLEGDLRRELTP
                     KDPNDSRNVVLEIRAGTGGDEASLFAAELFRMYSRYAEQQGWRIEVIDLSESGVGGVK
                     EVSAIVEGRGAYSRLKYEGGVHRVQRVPETEASGRIHTSAVTVAVLPEADDVEVEVDE
                     KDLRIDRFCASGPGGQGVNTTYSAVRLTHLPTGIVVSCQDERSQIKNKAKALRVLKAR
                     ILEIEREKAESAMSEERRKMVGSGDRSEKIRTYNFPQNRVTDHRIGVTVHQLPSVLQG
                     DLDSLIEPLVAHYQAQKMEEQARTH"
     gene            complement(2106..3134)
                     /locus_tag="VEL74_11075"
     CDS             complement(2106..3134)
                     /locus_tag="VEL74_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HYG63114.1"
                     /translation="MSASKSPESELLVGGQALIEGVMMRSPGAWGIAVRRPDGSLALQ
                     RGKVNPWGRRFPILKLPILRGCAVLFQSLALGIRALNFSAAEAMPEEMKQKDPGDNGK
                     ADTSGLAIAGAMVTGLGLGVVVFLLLPLWGTQLAERFVFGPLSNVAFNLIDGGLRGVI
                     FLLYILAISRMKDIHRVFMYHGAEHKVVFAYEARGPLTVDAARVQSRLHPRCGTSFLL
                     FVLLVSVVLFAFIPKDAPFLIKFGGRLLLMPLVAGLSYEVLRLTARLRGLPLFSAMVA
                     PGLALQRITTQEPTDDMLEVAIVALEEALREDGLLPEAAPGTAAVTEATAPPAAATAA
                     TAAAALGA"
     gene            complement(3198..3407)
                     /gene="rpmE"
                     /locus_tag="VEL74_11080"
     CDS             complement(3198..3407)
                     /gene="rpmE"
                     /locus_tag="VEL74_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HYG63115.1"
                     /translation="MKKDMHPTLNLVTVHCACGNTFDTHSTKDELRLEVCSNCHPYFT
                     GRQKLLDTAGRVERFNRRYAGAKRA"
     gene            3652..3855
                     /locus_tag="VEL74_11085"
     CDS             3652..3855
                     /locus_tag="VEL74_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63116.1"
                     /translation="MKKPLTKLVFALALSTAALVTSAQPSHALYCGQRPGCTFQGVFD
                     YGGAICCTYDCNGLTRIGPCQQV"
     gene            complement(3931..>5036)
                     /locus_tag="VEL74_11090"
     CDS             complement(3931..>5036)
                     /locus_tag="VEL74_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63117.1"
                     /translation="RSLLVRPGDTFRGLMELTGPASLRYAARLAGVGQTGGMGVYLPV
                     LTSENMVPANRAVTLLGLLSAGTRTTDIAVVNLAHQAAQCTIALARADGTPIGSTATI
                     QVKPLSQRYFANALGGLVDPGTGVSDARATVSCTQGYYAYAMLANSANGEVAFVGPAG
                     NGDSALRVPGSNPNPNPTCGAGAICFTANGVVHRPTRANPVGRLSVNAPVGTFTRLRL
                     TMEITVGDWYIGDADGKHLIYWFVINRNLDMPGMLFFRGPEQYTVLGRHGIGLRHADK
                     LRIEQPFQAVPGRTYRIVNDYDMGRGVNTITITDLGSNQVTTLTSVPNVRQHTFKDGD
                     VFLLDMGFPEGRIADEVPSYNWTYSNVLLEVVP"
BASE COUNT          725 a         1670 c         1795 g          846 t
ORIGIN      
        1 ccacgctgcc agtcgcctgc gccgggcgcc cctcccctcg ggccccccgg cgcggttccg
       61 gagcaggatt acttcttgta gcggacgcgg acgccctggg cgagatcctc gggcttcatc
      121 ttctcgaggt tctcgatgtt gtactcccag atgagggtgt tcccctgctt gcgggtggca
      181 ttgtgctcga ccacctcgaa gggggcctcg atgcggtagg cgagccgcag gcccttgaac
      241 atctccgcca cctgcttctt catctcgggg tccatctccc ccatgggaga ctgctgctcg
      301 gcctcggccg cggggttcac ggtcttgctg gtcagcagga tcgtcgcccc ctcgtccttc
      361 acctcgagcc cgccgaacgg gctgtcgaag ggattctccg gaccgggcgc ggcctgcccc
      421 tgagcgtccg tcttgccggg gagcttgatg agcgccagct tcgagacgtt gtcgaactcg
      481 aagccgagcc ccaggtcgaa cccgagctcc ttctcggcga acgtgttctc gaccagcttg
      541 acccccggtg gcaggctctt gcggaacttg tccatctcgg cgttcatctt ggccttgtcc
      601 gggctggtgc cggtggactt gaagctcttc ttgagctcgg ccagctccgc gggggtggga
      661 tccccctcct gcccggccat ctgccgctgc atcctggcca tcatcaggac catgggctcc
      721 atgttcactt tcatggcaaa gccggccttg ccggacaggt ccttgttcag gctcagcttg
      781 ttctcgacgt cgaagcagcc ggtcagcgac aacgcggcaa cgcaggccag cacgatccgt
      841 ttcatgggtc tccctccttc tgaaaggcca gtctagcgca gggtgggggc gggggagagg
      901 gcgggattcg tccggcgcag gcgactggca gcgtggaagg gagggagggg gagcgcacac
      961 gaaggagccg agcaggacga atcccgccct cccccccgga ggggggcctc agtgcgtgcg
     1021 cgcctgctct tccatcttct gggcctgata atgagcgacc aacggctcga tcagcgagtc
     1081 caggtccccc tgcaggacgc tggggagctg gtggacggtg acgccgatgc gatgatcggt
     1141 gacccggttc tgcgggaagt tgtaggtgcg gatcttctcg gaccggtcgc cgctgcccac
     1201 catcttccgg cgctcctcgg acattgccga ctccgccttt tcgcgctcga tctcgagaat
     1261 gcgggccttc aacactcgta gcgccttggc cttgttcttg atctggctgc gctcgtcctg
     1321 gcagctcacc acgatgccgg tggggaggtg ggtcaggcgc accgccgagt aggtggtgtt
     1381 gaccccctgg ccgccggggc cggaggcgca gaagcggtcg atacgcaggt ccttctcgtc
     1441 gacctcgacc tcgacatcgt cggcctcggg gaggacggcg acggtcaccg ccgaggtgtg
     1501 gatgcggccg gaggcttcgg tctcggggac ccgctgcacg cggtggacgc ccccctcgta
     1561 cttgaggcgg ctgtaggcgc cgcgcccctc gacgatggcc gagacctcct tgacgccgcc
     1621 gacgccggac tcggagaggt cgatgacctc gatgcgccac ccctgctgct cggcgtagcg
     1681 gctgtacatg cggaacagct cggccgcgaa caggctggcc tcgtcgccgc cggtgccggc
     1741 gcggatctcg aggacgacgt tgcggctgtc gttcgggtcc ttcggggtga gctcgcggcg
     1801 caggtccccc tcgaggcgct cgaggcgctc cgccagctcg cggcgctcct catgggccat
     1861 ctcgaagagg tcgtcgtcct tgccgaggct ggcgaggagc tcgtcgttct cctcgatctg
     1921 gcgctggacg ctgcggtact cgcggaagag cccgaccacg tgcgcgatct cggaaagggt
     1981 ccggttggtc tccgcgtagg ccttgcggtc ggagagcagg gtgggatcgg tgagcttctg
     2041 ggtgagctcc tcgtgagtct tttcgagctg ggaaagctgt tcgagcatgg acttgtcctc
     2101 agagatcagg cgccgagggc cgcggcggcc gtcgcagcgg tggcggccgc gggaggcgca
     2161 gtggcctccg tcacggcggc cgtgccgggc gccgcctcgg gcagcagccc gtcctctcgc
     2221 agcgcctcct ccagggcgac gatcgccacc tccagcatgt cgtccgtggg ctcctgggtc
     2281 gtgatccgct ggagcgccag gccgggggcg accatggccg agaagagggg caggccgcgc
     2341 aggcgggcgg tcagccgcag cacctcgtac gagaggccgg cgaccagggg catcagcagc
     2401 agccggccgc cgaacttgat caggaacggc gcgtccttgg ggatgaaggc gaacaggacc
     2461 accgagacca gcagcacgaa cagcaggaag ctggtgccgc agcgcgggtg cagccggctc
     2521 tggacgcggg cggcgtccac cgtcagcggg ccacgggcct cgtaggcgaa gaccaccttg
     2581 tgctcggcgc cgtggtacat gaacacccgg tggatgtcct tcatccgcga gatggccagg
     2641 atatacagca ggaagatgac cccccgcagg ccgccgtcga tgaggttgaa ggcgacgttc
     2701 gagagggggc caaagacgaa gcgctcggcg agctgggtgc cccacagcgg caggagcagg
     2761 aagacgacga cgccgagccc aagcccggtc accatggcgc cggcgatcgc caggcccgag
     2821 gtgtccgcct tgccgttatc cccggggtcc ttctgcttca tctcctcggg catcgcctcg
     2881 gcggccgaga agttgagcgc gcggatgccc agcgccagcg actggaagag gacggcgcac
     2941 ccccgcagga tggggagctt gaggatgggg aagcgccgtc cccacgggtt caccttgccg
     3001 cgctggaggg cgagcgagcc gtcggggcgg cgcacggcga tgccccaggc gccgggggag
     3061 cgcatcatca ccccctcgat cagcgcttgg ccaccgacca gcagctcgct ctccgggctc
     3121 ttgctcgctg acataccgtg gactcccgaa gggcccgccg cctctcggtc catgggacgg
     3181 cgaggcggcg gcgcggctca ggcgcgcttg gcgcccgcgt accggcggtt gaagcgctcg
     3241 acgcggcccg cggtatccag cagcttctgc cggccggtga agtacgggtg gcagttgctg
     3301 cagacttcca ggcgcagctc gtccttggtg gaatgggtgt cgaaggtgtt gccgcacgcg
     3361 cagtgcacgg tcaccaaatt cagggtcgga tgcatgtctt tcttcacggc cttgctctcc
     3421 aggtggagtc ggtctcggaa tcgcagaggg acttttgccc aagaacccgg aagcctaccc
     3481 gaggacggca ttcttttcaa gggttgcggg ccggggctcc ctccggcgtt ctataagcgc
     3541 ttgcgggagt ggaaaattcc ggagggcccc tcccctggac ccggcaaccg gcgggcgggc
     3601 actggagaga gtagaggctg tgctcggcct cgattctgaa aggagacgtc gatgaagaag
     3661 cccctcacca agctcgtctt cgccctggcc ctctccaccg ccgccctggt cacctcggcc
     3721 cagccctctc acgccctcta ctgcgggcag aggccgggct gcaccttcca aggcgtcttc
     3781 gactacggcg gcgccatctg ctgcacgtac gactgcaacg gcctgacccg gatcggcccg
     3841 tgccagcagg tttgacccca ccttcagaag gaagggcgcc ggtctcccgg cgcccttcgc
     3901 gcgttcagag gcgaggcgga gggcctcctc ttacgggacc acctcgagca ggacgttgct
     3961 gtaggtccag ttgtaggacg gcacctcgtc ggcgatccgc ccctccggga agcccatgtc
     4021 gaggaggaag acgtcgccgt ccttgaaggt gtgctggcgc acgttgggga cgctggtgag
     4081 ggtggtcacc tggttggagc ccaggtcggt gatggtgatg gtgttgacgc cgcgccccat
     4141 gtcgtagtcg ttgacgatcc ggtaggtgcg gccgggcacc gcctggaagg gctgctctat
     4201 ccgcagcttg tcggcgtggc gcaggccgat gccgtggcgg cccaggacgg tgtactgctc
     4261 cgggccccgg aagaagagca tgccgggcat gtccaggttg cggttgatga cgaaccagta
     4321 gatgaggtgc ttgccgtccg cgtccccgat gtaccagtcg cccaccgtga tctccatggt
     4381 cagccgcagg cgggtgaagg tgccgaccgg cgcgttgacg ctcaggcggc ctacggggtt
     4441 ggcgcgcgtc ggccggtgga ccacgccgtt ggccgtgaag cagatggcgc ccgcgccgca
     4501 ggtcgggttg gggttcgggt tggagcccgg cacccgcagg gccgagtcgc cgttacccgc
     4561 cgggccgacg aaggccacct cgccgttggc gctgttggcg agcatggcgt acgcgtagta
     4621 cccctgggtg cagctcaccg tcgcccgcgc gtcgctcacc ccggtcccgg ggtccaccag
     4681 gccgcccagg gcgttggcga aatagcgctg ggacagcggc ttgacctgga tggtggcggt
     4741 cgagccgatg ggggtgccgt ccgcccgtgc cagggcgatc gtgcactgcg ccgcctgatg
     4801 ggcgaggttg accaccgcga tgtcggtggt gcgcgtgccg gcggagagca gccccagcag
     4861 ggtgaccgcc cggttcgccg gcaccatgtt ctccgaggtg agcaccggca ggtagacgcc
     4921 catgccgccg gtctggccga cgccggcgag gcgcgccgcg tagcgcaggg aggcggggcc
     4981 ggtcagctcc atcaggccgc ggaacgtgtc gcccggccgc accagcagcg agcggc
//
LOCUS       DASPJI010000411         7524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_834314, whole genome shotgun sequence.
ACCESSION   DASPJI010000411 DASPJI010000000
VERSION     DASPJI010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7524)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7524
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834314"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..591)
                     /locus_tag="VEL74_11095"
     CDS             complement(295..591)
                     /locus_tag="VEL74_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63118.1"
                     /translation="MSSQSPRRTLVLAALLAVLAVPALQAQPLTVPSMDNWLSRLWSH
                     VEVLFEKEGASIDPDGRNAAPPAPPSTGGDAGVSIDPDGIHAAGGEGVIAGPKS"
     gene            794..2593
                     /locus_tag="VEL74_11100"
     CDS             794..2593
                     /locus_tag="VEL74_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HYG63119.1"
                     /translation="MPVNTLSELFLKAAGYQKPDCLLQKVGGTFQPVSTAELVDRVRR
                     LAKALVDLGVQRGDRVALMSENGPHWPTVDFATLCMGGVLVPIYPTLLPEQAAYIAGN
                     CGAKVVFAETTAHLEGLLAHASQLSEVRQFVLIRGTSSDPRVITMADLVERGAGVDPA
                     WFEREARAAKPDDLATLIYTSGTTGTPKGVMLTHGNIASNVEAGLSVLGLEGSYTALS
                     FLPLSHSFERTVDYCYFHTGCTIAYAESVQAVAQNLQEVNPHVFVAVPRVYEKVLSRV
                     QETVASGSPVKQKIFAWAQGVAREALPYRLKGKTPGGLLGVKLGLADKLVFGKIKTRL
                     GSRFQFAISGGAPLAREVAEFFWGIGVPIYEGYGLSETSPVISVNAVGAVKLGTVGRP
                     IPGVQVRIAPDGEILSKGPHIMKGYWNMPNETSEVIDAEGWFHSGDIGEVDSDGFLRI
                     TDRKKELIVNAYGKNVAPAPIENALKASRFIGQAVVLGDRRKFLTALVVPDFEALRPW
                     AERQGIGAKENAALIQDAQVRALIGKEVEQVNAGLAHFERIVGWELMPAEWTLETGEL
                     TPTQKVKRRVINQKYGDVIERLYGETEARAGAA"
     gene            2609..4750
                     /locus_tag="VEL74_11105"
     CDS             2609..4750
                     /locus_tag="VEL74_11105"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HYG63120.1"
                     /translation="MSEIFRLEVGGDRLATLTFYLPGKKLNVFTRAALAELEERIDEL
                     GRRRDIGCLILLSGKDGGFIAGADVEEIARVADPLEAEAGARLGQRLFAAWEALPFPT
                     VAAIRGVCVGGGTELSLASTWRVASDRKDTKIGLPEVRLGILPAWGGSNRLPRLIGIP
                     EALEMILTGRNLSGRRALKVGLIDALIPDARFLDHVRDFALDKRDMPRKEGSGGVDLK
                     ELLLEKNPLGRKILFDQARKKTLEQTRGHYPAPLRAIEVVRIGLEDGRKAGFDAEARA
                     LADLATAQTSKNLVHVFGLQEGAKRAKGVPGGEAREVRRAAVLGAGVMGGGIAQIIAQ
                     EAGIPVRMKDLNDEALVSGMAHASGLFDRLVKKRRLESAEARRRMALLLPSLDYSGFG
                     GADLVVEAIVEKMDVKQDAFAELARHVPETAVLASNTSSLSVAGIGAKTPNPGRVVGM
                     HFFNPVHKMPLVEVIATDVSDPAAVNTVFDFTRRLGKIPVLVKDAPGFLVNRLLMFYS
                     IEAYWLLHEGHRIEDLDTAMLDWGMPVGPLALGDDVGLDVAIKVAHILGEAFADRLPL
                     PPWLNKMTESGRLGVKNGSGFYRYDGKERKGPDPSAYEVLGVRPRVENPDPNALAERM
                     VLPMVNEAARCLEEGVVRSAAELDLAMIFATGFPPFRGGLCRWADHEGLPRLVATLER
                     LEVAAGERFRPSDALRATAEAGGFYARFGGG"
     gene            4928..6025
                     /locus_tag="VEL74_11110"
     CDS             4928..6025
                     /locus_tag="VEL74_11110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG63121.1"
                     /translation="MNRTIRAVALSMLAALTIGGAAFAQDAQRGEFGEEVTVNEVLLD
                     VLVTDNKGNVVVGLNEDDFVVKEGGKPVDLTGITFYSSNRLTESPESLAQKGIRGVDT
                     APEDRYFILFFQDQKNAASEAPVLLTKQMEANQRAREWIGELGLHDHVAVAGYDQKLK
                     IYQDFTTDKAALVRALGQAMKGKELESWPSRQQQAQGPSILDTLPTGKELRNQTTNIY
                     DAIRVLAEATADIRGRKNLLLFTPGFGVVRTGQLRPDLRYYPDMADALNDANVAVYPI
                     DLVPEGARPALNETLNQIAEDTGGQYLFSFVNFITPLRQIGDENGGYYLLSYRSEHPA
                     GKSGFQKVEVDTANPQFRIRTREGYSYGSGK"
     gene            6134..7207
                     /locus_tag="VEL74_11115"
     CDS             6134..7207
                     /locus_tag="VEL74_11115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG63122.1"
                     /translation="MMHAVRKNAALGLAALASLIAFPSLAARAQPPAEPSAEISSSDP
                     GDSPQALLDVIVTGADGKPVLGLEADDFVLKVGGKPVPVQDVTFSSVRRLVKNTGLNT
                     DEIPEERYFILLFEKDFLASMTRNTQLWEQRKRVLEVAKKWLRLLEPNDWVAVATYDQ
                     SLELLQDFTRDPDVLISALDRTLVAKAGEPSPGPSPNSPTLAALPKGKELVARTSSIH
                     EALEEIARATRGIKARKSLLLITDGISEGSGGSRQVSMGLPYLDALRELNAANVSIYP
                     VDIWERGSPQRALLEFAEDTGGKITPRVGTSFEGVLTDSAAYYLLHYPPPQTGRTSKK
                     VEVTTTRPGLVVRTRSGVSQGKG"
     gene            complement(7216..>7524)
                     /locus_tag="VEL74_11120"
     CDS             complement(7216..>7524)
                     /locus_tag="VEL74_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYG63123.1"
                     /translation="ALADPLTREGIRYGMLSGLWAAESLLAGRPEAYPERLAAELAGE
                     LDRAGRACGLFFEGPIGQWMVPVARVHPGIRRVLADLLSCRQPYRGLKRRLLSAAVGG
                     "
BASE COUNT         1229 a         2680 c         2519 g         1096 t
ORIGIN      
        1 ggcctacctc ctcctggagc cgcagaatct cctggtacct ctcccggcag gcggggcaat
       61 gctccagcag atgggggacc acctcggcca ccaccttctc gtgctccagc cctccagaga
      121 gcagcttcgc caagtcctcg atcaagatat gagcgtgctc caagagatcc tctcctccag
      181 ccggcttgcc ggtcagtcgc tcctcactag ctttagttac cgtgcacggc taatccgtga
      241 cacccacggc aaagaaagag aggggccggc ccggagaccg gcccctcccc cggatcagct
      301 cttggggccg gcgatcacac cctcccctcc ggcggcgtgg atgccatcag gatcgatcga
      361 caccccggcg tcgcctccgg tgctgggagg agcgggcggc gcggcgttgc ggccatcggg
      421 gtcgatggac gccccctcct tctcgaacag cacctcgacg tggctccaca accgcgaaag
      481 ccagttgtcc atcgagggga ccgtcagcgg ctgggcctgc agagcgggga cggccaggac
      541 ggcgaggagg gcggcgagca cgagggtacg gcggggggat tgcgaagaca tggattcctc
      601 tcttccggga cggatctccg gcgccggcac cacgccagcc gggtccgtcc tctataatag
      661 gttgcctgag acggcaaaaa cgcgcacccc cctccaaaga gatcccctcc ttcctcgccg
      721 ccgcctcgtc ggtacctcgt cggcaagcgc cgcccgcgat agaatgccgg cggacatcac
      781 gccaggaggc cccatgccgg tcaatacgtt gtcggagctg tttctaaagg ccgccggata
      841 ccagaagccg gactgccttc tccagaaggt cggaggtacc ttccagcccg tctcgacggc
      901 ggagctggtg gaccgggtcc ggcgcctcgc caaggccctc gtcgacctcg gggtccagcg
      961 cggcgaccgc gtcgccctga tgtccgagaa cggcccgcac tggccgacgg tggacttcgc
     1021 caccctctgc atgggcggcg tgctggtgcc catctacccg acgctgctgc ccgagcaggc
     1081 cgcctacatc gcgggcaact gcggcgccaa ggtggtcttc gccgagacca cggcgcacct
     1141 cgagggcctc ctcgcccacg cctcccagct ctccgaggtc cggcagttcg tgctgatccg
     1201 cggcacctcg tccgacccgc gggtgatcac catggccgac ctcgtcgagc gcggcgccgg
     1261 cgtcgacccc gcctggttcg agcgcgaagc ccgcgccgcc aagccggacg acctggcgac
     1321 cctcatctac acgagcggca ccaccggcac ccccaagggg gtcatgctca cccacggaaa
     1381 catcgcctcg aacgtcgagg cgggcctctc cgtgctgggg ctcgagggga gctacaccgc
     1441 gctctccttc ctccccctct cgcactcctt cgagcggacg gtcgactact gctacttcca
     1501 caccggctgc accatcgcct acgccgagtc ggtgcaggcg gtggcgcaga acctccagga
     1561 ggtcaacccg cacgtcttcg tcgcggtacc gcgcgtctat gagaaggtgc tctcccgggt
     1621 gcaggagacg gtggcctccg gctcgccggt caaacagaag atcttcgcct gggcccaggg
     1681 cgtggcccgt gaggcgctcc cctatcggct gaagggcaag acgcccggcg gcctgctcgg
     1741 agtcaagctc ggcctcgccg acaagctggt cttcggcaag atcaagacgc gcctgggcag
     1801 ccgctttcag ttcgccatct ccggcggcgc gccgctcgcc cgcgaggtgg ccgagttctt
     1861 ctggggcatc ggagtcccga tctatgaagg ctacggcctg tcggagacct cgcccgtcat
     1921 ctcggtcaac gccgtgggcg cggtcaagct cggcaccgtc ggccggccga tccccggcgt
     1981 ccaggtgcgg atcgcgccgg acggcgagat cctctccaag ggcccgcaca tcatgaaggg
     2041 ctactggaac atgccgaacg agacctccga ggtcatcgac gccgagggct ggttccacag
     2101 cggcgacatc ggcgaggtgg acagcgacgg cttcctgcgc atcaccgacc gcaagaagga
     2161 gctgatcgtc aacgcctacg gcaagaacgt ggcgccggcg ccgatcgaga acgctctgaa
     2221 ggccagccgc ttcatcggcc aggccgtggt cctcggcgac cgccgcaagt tcctgaccgc
     2281 cctggtcgtg cccgacttcg aggcgctgcg cccgtgggcc gagcggcagg ggatcggtgc
     2341 caaggaaaac gccgcgctca ttcaggatgc ccaggtccgc gccctgatcg gcaaggaggt
     2401 cgagcaggtc aacgccggcc tcgcccactt cgagcgcatc gtcggctggg agctgatgcc
     2461 ggccgagtgg accctggaga ccggcgagct gaccccgacg cagaaggtca agcggcgggt
     2521 gatcaaccag aaatacggcg acgtcatcga gcggctctac ggcgagaccg aggcgcgcgc
     2581 cggcgcggcc tgagaagggg ggttgggggt gagcgagatc tttcggctgg aggtgggcgg
     2641 ggaccggctc gccaccctca cgttctacct gccgggcaag aagctgaacg tcttcacccg
     2701 cgccgcgctg gcggagctgg aggagcggat cgacgagctg ggccggcgcc gggacatcgg
     2761 ctgcctgatc ctcctctccg gcaaggacgg cggcttcatc gccggcgccg acgtcgagga
     2821 gatcgcccgc gtcgccgacc cgctggaggc cgaggccggc gcgcgcctcg gccagcgcct
     2881 gttcgccgcc tgggaggcgc tccccttccc caccgtcgcc gccatccgcg gtgtctgcgt
     2941 cggcggcggc accgagctct ccctcgcctc cacctggcgg gtggcgagcg accgcaagga
     3001 caccaagatc ggcctgcccg aggtgcggct cggcatcctc cccgcctggg ggggctccaa
     3061 ccgcctgccc cgcctcatcg gcatccccga agcgctggag atgatcctca ccggccgcaa
     3121 tctgtccggc cgccgggccc tcaaggtcgg cctgatcgac gccctgatcc ccgacgcccg
     3181 cttcctcgac cacgtgcgcg acttcgccct ggacaaaagg gacatgccgc gcaaggaggg
     3241 gagcggcggc gtggacctca aggagctgct gctcgagaag aacccgctgg gccgcaagat
     3301 cctcttcgac caggcgcgca agaagacgct ggaacagacg cgcggccact acccggcgcc
     3361 gttgcgggcc atcgaggtgg tgcgcatcgg cctggaggac ggccgcaagg ccggcttcga
     3421 cgccgaggcg cgggccctcg ccgacctggc gaccgcgcag acgtcgaaga acctggtcca
     3481 cgtcttcggc ctgcaggagg gggcgaagcg ggccaagggg gtgccggggg gcgaggcgcg
     3541 ggaggtgcgc cgggccgcgg tcctgggcgc cggcgtcatg ggcggcggca tcgcccagat
     3601 catcgcgcag gaggccggca tcccggtccg catgaaggac ctcaacgacg aggcgctggt
     3661 cagcggcatg gcccacgcct ccggcctctt cgaccggctg gtcaagaagc ggcggctgga
     3721 gagcgccgag gcgcggcgcc gcatggccct gctcctcccc agcctcgact actccggctt
     3781 cggcggcgcc gacctggtgg tcgaggcgat cgtcgagaag atggacgtca agcaggacgc
     3841 cttcgccgag ctggcgcgcc acgtgccgga gacggcggtg ctcgcctcca acacctcgtc
     3901 gctgtcggtc gccggcatcg gcgccaagac cccgaacccc ggccgggtgg tggggatgca
     3961 cttcttcaac ccggtccaca agatgccgct ggtcgaggtg atcgccaccg acgtcagcga
     4021 cccggcggcg gtcaacaccg tcttcgactt cacccgccgg ctcggcaaga tcccggtgct
     4081 ggtgaaggac gcccccggct tcctggtcaa ccgcctgctg atgttctact cgatcgaggc
     4141 ctactggctg ctgcacgagg ggcaccgcat cgaggacctg gacacggcga tgctcgactg
     4201 gggcatgccg gtgggcccgc tggcgctggg ggacgacgtg ggcctcgacg tggcgatcaa
     4261 ggtggcccac atcctgggcg aggcgttcgc ggaccgcctg cccctccccc cctggctgaa
     4321 caagatgacg gagagcggcc ggctgggggt caagaacggc tcgggcttct accgttacga
     4381 cggcaaggag cgcaagggtc cggacccctc cgcctacgag gtcctggggg tgcggccgcg
     4441 ggtggagaac ccggacccca acgccctcgc cgagcgcatg gtgctgccga tggtcaacga
     4501 ggcggcgcgc tgcctggagg agggtgtggt gcggagcgcc gccgagctcg acctcgccat
     4561 gatcttcgcc accggcttcc ctccgttccg cggcggcctc tgccgctggg cggaccacga
     4621 ggggctcccc cggctcgtcg ccaccctgga gcggctggag gtcgccgccg gcgagcgctt
     4681 ccgtccctcc gacgccctcc gggccaccgc cgaggcgggg ggcttctacg cccggttcgg
     4741 gggcggatag gcgtcctcta tcgaggacgg cgggaggccg ccgccgtcct ctccgggcac
     4801 cgggccccgg accggcctcc gaggctcaaa gtcaagaata ttctagggtt tacccgttgt
     4861 tagcctctac aggaagcact ggcgcggcct ttgctctcct gctttctccg tgaaagggag
     4921 gttacaaatg aatcgaacga tccgagccgt cgcgctttcc atgctcgcgg ccttgacgat
     4981 aggcggtgca gccttcgccc aggatgccca gcgtggggag ttcggcgaag aggtgacggt
     5041 caacgaggtg ctgctcgacg tcctggtgac cgacaacaag ggcaacgtcg tggtcggcct
     5101 caacgaggac gacttcgtgg tcaaggaggg cggcaagccc gtcgacctga ccggcatcac
     5161 gttctacagc agcaaccggc tgaccgagtc ccccgagtcc ctggcccaga aggggatccg
     5221 gggcgtggac acggcccccg aggaccgcta cttcatcctg ttcttccagg accagaagaa
     5281 cgcggcgtcc gaggcgcccg tcctcctgac caagcagatg gaggccaacc agcgggcccg
     5341 cgagtggatc ggcgagttgg gtctccacga tcacgtggcg gtggccggct acgaccagaa
     5401 gctcaagatc taccaggact tcaccaccga caaggcggcg ctggtgcggg ccctcggcca
     5461 ggcgatgaag ggcaaggagc tggagagctg gccgtcgcgg cagcagcagg cccagggccc
     5521 gtcgatcctg gacaccctgc ccacgggcaa ggagctgcgc aaccagacca ccaacatcta
     5581 tgacgccatc cgcgtgctgg cggaggccac cgccgacatc cgaggccgca agaacctgct
     5641 gctcttcacc cccggcttcg gcgtggtgcg gacgggtcag ctccggcccg acctccggta
     5701 ctacccggac atggccgacg cgctgaacga cgccaacgtg gcggtctacc cgatcgacct
     5761 ggtgcccgag ggggcgcggc cggcgctcaa cgaaacgctg aaccagatcg ccgaggacac
     5821 cggcggccag tacctgttca gcttcgtcaa cttcatcacg ccgttgcgcc agatcggcga
     5881 cgagaacggc ggctactacc tgctgagcta ccggagcgag catccggccg gcaagtcggg
     5941 gttccagaag gtcgaggtcg acacggccaa cccccagttc cggatccgga cccgcgaagg
     6001 ctactcctac ggttcgggca agtaagcccg accctggcgg ggccgctcca ttcggggcgg
     6061 ccccggccct ttccttcatc ccccgcttct ttgacgtgag cttttggttc cttctatttt
     6121 caacaggagg ttcatgatgc acgctgtcag gaagaatgcc gccctcgggc tggccgcgct
     6181 cgcctcgctc atcgccttcc cctctctcgc cgcccgggcg cagccgcccg cggagccctc
     6241 cgccgagatc tcctcgtccg atccgggaga cagcccccaa gccctgctgg acgtcatcgt
     6301 caccggcgcc gacggcaagc ccgtcctggg cctggaggcg gacgacttcg tcctgaaggt
     6361 cggaggcaag ccagtgcccg tccaggacgt gaccttctcc agcgtccggc ggctggtgaa
     6421 gaacacgggc ctcaacaccg acgagatccc cgaggagcgg tacttcatcc ttctcttcga
     6481 gaaggacttt ttggcgtcca tgacccggaa tacccagctc tgggagcaga ggaaaagggt
     6541 gctcgaggtc gccaagaagt ggctgaggct gctcgagccc aacgactggg tggcggtggc
     6601 cacctacgac cagagcctcg agttgctcca ggacttcacc cgggaccccg atgtcctgat
     6661 ctccgccctg gaccgcaccc tggtagccaa agccggcgag ccgagccccg gcccatctcc
     6721 gaacagcccc actctggcag ccctgcccaa ggggaaggag ttggtcgccc gcacctcctc
     6781 gattcacgag gcgctggaag agatcgccag agccacgcgc ggtatcaagg cccggaagag
     6841 cctcctcctg attaccgatg ggatctccga agggtctggt gggtcgcgcc aggtctcgat
     6901 gggcctcccc tacctcgacg cgctccggga gctcaacgcc gcgaacgtgt ccatctaccc
     6961 ggtggatata tgggagaggg ggtctcctca gcgcgcgctc ctcgaattcg cggaggatac
     7021 cggcggcaag atcactccga gggtaggcac gagcttcgaa ggcgtgctga cggactccgc
     7081 ggcctactac ctgctccact acccgccacc ccaaaccggc cgcacctcga agaaggtcga
     7141 ggtcaccacc acccgcccgg gcctggtggt ccggacgcgg agcggcgtgt cgcaagggaa
     7201 gggctgagcg gctgactacc cacctaccgc ggccgagagc agacggcgct tgaggccgcg
     7261 gtagggctgg cggcaggaca ggaggtccgc cagcacccgc cggatgcccg ggtggacgcg
     7321 ggcgaccggc accatccact gcccgatggg accctcgaag aagaggccgc aggcgcggcc
     7381 ggcccggtcg agctcgccgg ccagttcggc ggcgagacgc tccgggtagg cttcgggcct
     7441 ccccgccagc agactctccg ccgcccacag cccggacagc atcccatagc gtatgccttc
     7501 ccgggtcagc ggatcggcga gagc
//
LOCUS       DASPJI010000412         5644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_320773, whole genome shotgun sequence.
ACCESSION   DASPJI010000412 DASPJI010000000
VERSION     DASPJI010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320773"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>5644
                     /locus_tag="VEL74_11125"
     CDS             <1..>5644
                     /locus_tag="VEL74_11125"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63124.1"
                     /translation="EYRDGVCLHELIEEQVERTPGAVAVTFNGLETTYRELNRRANRL
                     AHHLRRLGVGPECLVGVVAERSTEMVTGLVATLKAGGAYVPLDPGYPRDRVQFMIEDA
                     GIGVLLTESHLEAALPHHSARVVLLDAESSWESLRISNPEAGARPESPAYAIYTSGST
                     GRPKGAMIPHRGIVNRLLWMQDAYGLGPDDRVLQKTPFSFDVSVWELFWPLITGARLV
                     MAPPGAHQDAAWLARIIHDEEITTLHFVPSMLQLFLEGKDLATACRTVRRVMASGEAL
                     PYELQERALERIGAELHNLYGPTEASVDVSFHACERGSARRTVPIGRPISNISLHILD
                     RHSEPVPPGIPGELLIGGVGLARGYLHRPALTAERFVPDPLGDPGERLYRTGDLARFR
                     PDGAIEFLGRIDHQIKIRGVRVELGEIEAALDHHPGVREAVVMARTEGVGETRLVAYL
                     VASGEAAPSAGELRSFLRDSLPEAMVPSAFVILPAFPLTPSGKVDRRALPAPDFEGAS
                     RETTPFAEPRTPLEERLAGMWRELLRVERLGIHDSFFELGGDSIQGAMFINRLQEELG
                     QIVYVMALFDAPTVAGFAAYLERSYPVAVVRLGGRAAGDQAGEGGPVETAAALESLHA
                     AVVRRLGRTPEEPEVPRALPKNPRAVFVLAPFRSGTTLFRVMLAGHPRLFAPPELELL
                     AFRTMGERRRAYMGRNSFALEGLLRAVMELRGCDADEARAWVAGFEDRDLPVQSFYGF
                     LQEESGGRFLVDKTPSYPLDPATLRRAEEMFEEPLYLHLVRHPRATADSYVEAHMDRV
                     YDFPFPPNEQADLVWLLSHRNIVEFLSGVPAERVHRLHFEDLVKAPGESIEAVCAFLG
                     LPFEPAMLDPYQGRRMTDGLHSESRMMGDPKFHRHRGIEAKVADRHQEAAHRGLRAET
                     LELAALLGYPVEEPKPAPEVLGPRPVPRAVGDEDIEMALSYSQERLWFLAQLDPGSPA
                     YNMPATVHLEGILDVPSLARSFGEVRRRHEVLRTTFPSAGDRPVLRVADVADIAGALP
                     VIDLSELPETRQEMERLAFAEGRRPFDLERGPMLRTTLLRLGSAEHALLVTMHHIVSD
                     GWTIGILIRELVALYSAFSQGLPSPLPELPLQYADYAVWQRRWLDGEAMAKHLDYWRQ
                     RLAGRLPALEMPTDRPRPAYQTFGGARLSRTLSAEASQELREWSRRQGTTPFLTLLAG
                     FNALLHRYTAQDDLLLGVPIANRNRLEVENLIGFFLNMVVQRTDVSGDPTLRELLGRV
                     SEGFLGSVPHQEVPFEKIVEALQPERDLSRAPIFQVQFSLQNTPSAALELPGLKLELL
                     EITNRTTKFDFTVFLFDNPDGLKTTLEYNTDLFDAVTIARLLGHWETLLAGVPANAER
                     RLSDLPVLTAAELGQLLGDWSGPAARPGAFPAQTCLHRLFEERARRDSKAVAVVYEVQ
                     EISYGRLNERANRLARRLRALGVGPDVPVALCVERSIDMIVGLLGILKAGGAYVPLDP
                     AYPPDRLTYVLEDALAGSRAPVLVTQERLAGLFGEELHFPVLKLDARLDADRALLAAE
                     SGDDLEEAGGPWPESLAYVIYTSGSTGRPKGVPVTHANAVRLLEATELWFGFGPDDVW
                     TMFHSFAFDFSVWEIWGALAYGGRLVIVPRETTLSPASFHELLATEGVTVLNQTPSAF
                     RQLVQLDRAEAHRRLDLRWVIFGGEALELSALAPWFARHGDETPRLINMYGITETTVH
                     VTYRPISQGDLRTPGHSPVGAPIPDLSVHLLGPRGELVPVGVPGEIHVGGAGVARGYL
                     GRPALTAERFVPDPLAGQPGARLYRSGDLARRRPDGDLEYLGRIDHQVKIRGFRIELG
                     EIEAALARHSGVAATVVMARPGASGAGTGADRRLVAWVV"
BASE COUNT          753 a         2057 c         1976 g          858 t
ORIGIN      
        1 tggagtaccg ggacggagtc tgcctgcacg agctgatcga ggagcaggtg gagcggaccc
       61 ccggtgccgt ggcggtcacg ttcaatggtc tggagacgac ctaccgcgag ctcaaccggc
      121 gcgccaaccg gctggcgcat cacctgcgcc ggctgggggt ggggccggag tgcctggtgg
      181 gcgtggtggc cgagcgctcc accgagatgg ttaccggcct cgtggcgacg ctcaaggccg
      241 gaggcgccta cgtccccctc gatccgggct atccgcgcga ccgcgtgcag ttcatgatcg
      301 aggacgcggg gatcggcgtc ctcctcaccg agagccacct cgaagccgcg ctgccgcatc
      361 actctgcgcg tgtcgtgctg ctggacgcgg agtcttcgtg ggaatcgttg cggatcagca
      421 accccgaagc gggggcccgt cccgagagcc cggcctacgc catctacacc tcgggatcga
      481 ccggccggcc gaagggggcg atgatcccgc accgcggcat cgtcaaccgg ctgctgtgga
      541 tgcaggacgc ctatgggctg gggccggacg accgcgtgct ccagaagacg ccgttcagct
      601 tcgacgtctc ggtctgggag ctcttctggc cgctgatcac cggcgcgcgg ctggtgatgg
      661 cccccccggg cgctcatcag gacgcggcct ggctggcgcg catcatccac gacgaggaga
      721 tcaccaccct tcacttcgtc ccctcgatgc tccagctctt cctggagggg aaggatctgg
      781 ccaccgcctg ccgcacggtc cggcgggtga tggcgagcgg cgaggcgctg ccgtacgagc
      841 tgcaggagcg ggccctcgag cggatcggag cggagctgca caatctctac ggccccaccg
      901 aggcgtcggt ggacgtcagc ttccacgcct gcgagcgggg gagcgcccgg cgcacggtcc
      961 ccatcgggcg gccgatctcg aacatctctc tccacatcct cgaccgccac agcgagccgg
     1021 tgccgccggg aatcccgggc gagctgctca tcggcggcgt gggcctggcg cgcggctatc
     1081 tgcaccggcc cgcgctcacc gcggagaggt tcgtccccga tcctctgggg gatcccggcg
     1141 agcggctgta ccgcacgggc gacctcgccc gcttccggcc ggacggcgcc atcgagttcc
     1201 tcggccgcat cgatcaccag atcaagatcc gcggcgtgcg cgtcgagctg ggggagatcg
     1261 aggcggcgct cgaccatcac cccggcgtgc gcgaggccgt cgtcatggcc cgtaccgagg
     1321 gagtggggga gacgcggctg gtggcctatc tggtggcgag cggcgaggcg gcgccgtcgg
     1381 ccggggagct gcggagcttc ctgcgcgaca gcctgcccga ggctatggtg ccctcggcgt
     1441 tcgtgatcct gccggcgttc cccctgaccc ccagcggcaa ggtcgaccgc cgcgccctgc
     1501 ccgctcccga tttcgagggc gccagccggg agacgacgcc gttcgcggag ccgcgcacgc
     1561 cgctcgagga gcgcctggcc ggcatgtggc gcgagctgct gcgcgtcgag cggctgggta
     1621 tccacgacag cttcttcgag ctgggcggcg attccatcca gggggcgatg ttcatcaacc
     1681 ggttgcagga ggagctgggc cagatcgtct acgtgatggc cctgttcgac gcgcccaccg
     1741 tcgccggctt cgccgcctat ctggagcgga gctatcccgt ggctgtggtg cgtctgggtg
     1801 gccgggcggc cggcgaccag gccggggagg gcgggccggt ggaaacggcc gcggcgctcg
     1861 aatccttgca tgccgcggtg gtccggcgcc tcggccggac gccggaggag cccgaggtac
     1921 cgcgggctct gccgaagaac ccgcgggcgg tcttcgtcct cgccccgttc cgctcgggca
     1981 cgaccctgtt ccgcgtcatg ctggccggcc acccgcgcct gttcgcgccg ccggagctgg
     2041 agctgctcgc cttccggacg atgggggagc gccggcgcgc ctacatgggc cgcaacagct
     2101 tcgccctgga agggctgctc cgcgcggtga tggagctgcg cggctgcgac gccgacgagg
     2161 cccgcgcctg ggtggccggt ttcgaggacc gggacctgcc ggtgcagagc ttctatggct
     2221 tcctccagga ggagagcggc gggcggttcc tggtggacaa gaccccgagc taccccctcg
     2281 accccgcaac cctgcggcgg gccgaggaga tgttcgagga gccgctctat ctccatctgg
     2341 tgcgccaccc cagggccacg gccgactcct acgtcgaggc ccacatggac cgggtctacg
     2401 acttcccgtt cccaccgaac gagcaggcgg acctggtctg gctcctcagc caccgcaaca
     2461 tcgtcgagtt cctctccggg gtgccggcgg agcgggtgca tcgcctgcat ttcgaggacc
     2521 tggtcaaggc gccgggcgag agcatcgagg cggtctgcgc gttcctcggg ctgccgttcg
     2581 agcccgccat gctcgatccc taccaggggc gccgcatgac cgacggcctc cactccgagt
     2641 cgcggatgat gggagacccg aagttccatc gccaccgcgg catcgaggcc aaggtcgccg
     2701 accggcatca ggaggccgcc caccgcgggc tgcgcgccga gacgttggag ctggccgccc
     2761 tgctcggcta cccggtggag gagccgaagc cggcgccgga ggttcttggc ccccgcccgg
     2821 tgccgagggc tgtcggggac gaggacatcg agatggcgct gtcgtactcc caggagcggc
     2881 tctggttcct cgcccagctc gatccgggga gcccggccta caacatgccg gcgacggtcc
     2941 acctcgaagg gatcctcgac gtccccagcc tcgcccgcag cttcggcgag gtgcggcgcc
     3001 gtcacgaggt gctgcgcacc accttcccct ccgccggcga ccggccggtc ctgcgggtcg
     3061 ccgacgtcgc cgacatcgcg ggcgccctgc cggtgatcga tctctcggag ctgcccgaga
     3121 cccggcagga gatggagcgc ctcgcctttg cggagggtcg gcggccgttc gacctggagc
     3181 gcgggccgat gctgcgcacc accctgctgc ggctcggcag cgccgagcac gcgctgctgg
     3241 tgacgatgca ccacatcgtc tcggacggct ggaccatcgg catcctgatc cgggagctgg
     3301 tggcgctcta cagcgccttc tcccaggggc tcccctcgcc gctgccggag ctccccctcc
     3361 agtacgcgga ctacgccgtc tggcagcgcc gttggttgga tggtgaggcc atggcaaagc
     3421 atctcgacta ctggcgccag cgcctcgccg ggcggctgcc ggccctggag atgcccaccg
     3481 accggccgcg tcccgcctac cagaccttcg gcggcgcgcg cctgtcgcgc acgctctccg
     3541 ccgaggcctc gcaggagctg cgcgagtgga gccgccggca agggacgacg cctttcctta
     3601 ccctgctcgc cgggttcaac gccctgctcc accgctacac cgcccaagac gacctgctgc
     3661 tcggcgtccc catcgccaac cgcaaccgcc tggaggtcga aaacctgatc ggtttcttcc
     3721 tcaacatggt ggtgcagcgt accgacgtct ccggcgatcc gacgctgcgc gagctgctcg
     3781 gccgggtgag cgagggcttc ctcggatcgg tgccgcacca ggaggtgccg ttcgaaaaaa
     3841 tcgtggaggc cctgcagccg gagcgcgacc tgagccgcgc gccgatcttc caggtgcaat
     3901 tctcgttgca gaacacaccg tcggcggcgc tggagctgcc cggcctgaag ctcgagctgc
     3961 tggagatcac caaccggacg accaagttcg acttcacggt cttcctgttc gacaacccgg
     4021 acggcctcaa gacgacgctc gaatacaaca ccgacctctt cgacgccgtc accatcgccc
     4081 gcctcctcgg ccactgggag acgctgctcg ccggcgtccc cgcgaacgcc gagcgtcggc
     4141 tgtcggacct ccccgtgctg acggcagccg agctcggcca gctcctcggt gattggagtg
     4201 gtcccgctgc ccggcccgga gcgttccccg ctcagacctg cctccaccgg ctgttcgagg
     4261 agcgggcgcg gcgcgactcc aaggcggtcg ccgtggtcta cgaggtccag gagatctcct
     4321 acggccggct caacgagcgc gccaaccgcc tggcgcgccg cctgcgcgcg ctaggcgtcg
     4381 gccccgacgt gccggtggcg ctgtgcgtcg agcgctccat cgacatgatc gtcggcctgc
     4441 tcggcatcct gaaggccggc ggcgcctatg tgccgctcga cccggcctat ccgccggacc
     4501 ggctgaccta cgtgctggag gacgccctcg ccggcagccg cgcgccggtc ctcgtcaccc
     4561 aggagcgcct ggccggcctc ttcggcgagg agctgcattt ccccgtcttg aagctggacg
     4621 cgcggctgga cgccgaccgt gccctgctgg cggccgagag cggtgacgac ctggaggagg
     4681 ccggcggtcc ctggcccgag agcctcgcct acgtcatcta cacctccggc tccaccgggc
     4741 ggcccaaggg ggtgccggtg acgcacgcca acgccgtgcg gctcctcgag gccaccgagc
     4801 tctggttcgg cttcggtccg gacgacgtct ggacgatgtt ccactcgttc gcattcgact
     4861 tctcggtatg ggagatctgg ggcgccctcg cctacggcgg ccggctggtg atcgtgccgc
     4921 gggagaccac gctgtccccc gcctcgttcc acgagctcct cgccaccgag ggggtcaccg
     4981 tcctcaatca gaccccctcg gcgttccggc agcttgtgca gctcgaccgg gccgaggcgc
     5041 accgccggct cgatctgcgc tgggtgatct tcggcggcga ggcgctggag ctgtcggccc
     5101 tggccccctg gttcgcccgc cacggcgatg agacgccgcg gctgatcaac atgtacggca
     5161 tcaccgaaac cacagtgcac gtcacctacc ggccgatcag tcagggggat ctcaggaccc
     5221 ccggccactc gccggtcggc gccccgatcc ccgatctgtc ggtgcacctg ctggggccgc
     5281 gcggcgagtt ggtgcccgtc ggcgtcccgg gtgagatcca cgtcggcggc gccggggtgg
     5341 cgcggggcta tctcggccgt cccgccctga ccgccgagcg cttcgtcccc gatccgttag
     5401 ccggccagcc cggcgcgcgg ctctaccgca gcggagactt ggcgcggcgg cggccggacg
     5461 gcgacctcga gtacctgggc cgcatcgatc atcaggtgaa gatccgcggc ttccgtatcg
     5521 agctggggga gatcgaagcc gccctcgccc gccactccgg agtggcggcg acggtcgtga
     5581 tggcccggcc cggcgcttcc ggagccggca cgggcgccga ccgacggctg gtggcctggg
     5641 tggt
//
LOCUS       DASPJI010000413         4841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_371072, whole genome shotgun sequence.
ACCESSION   DASPJI010000413 DASPJI010000000
VERSION     DASPJI010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371072"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..2011)
                     /gene="nuoD"
                     /locus_tag="VEL74_11130"
     CDS             complement(281..2011)
                     /gene="nuoD"
                     /locus_tag="VEL74_11130"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HYG63125.1"
                     /translation="MPTLAPPLPDRTTTAGNGDAAAEAVRAKFPGMITRQEGLDQETW
                     IVRREVLSDLARELKHNPATRYDLMLDLGGVDFPDREERFEAIYHLYSIQRNTRLRLK
                     VPLREDDAVLPSLIPVWKGCNWFERELYDMFGIRVEGHPNLRRILCHEGFVGHALRKD
                     YHPTKRWILTEDKIYKPHFDLPEGEDDLFERMTVNIGPSHPAMHGTFRLMAVLDGETI
                     VASDVEIGYLHRCFEKMSETHTYQQVIPYTDRLNYCSAIINNVGYCMTVEKLLGIEAP
                     PRAQHVRLMLSEFYRISDHIICIGTNLVDMGALTNFWWLFQPREEIIGLAESCSGARL
                     LPSYLRVGGIAIDVPSTFLTHAQHLVNTLPKYIDDVEALIMKNRIFRDRVDGVGVISR
                     EDAINFGFTGPCLRATGVPFDVRKASPYLGYDTYDWDVPVAEGGDTYARFLVRFEEMR
                     QSIRILQQAIDRGLPPGPVMVDNPYVALPPKEKVYNEMESLIYHFKLIMHGIQPPVGE
                     TYFQVEGGNGEVGFYIVSDGTKHPYRVRARPPCFTIFEAFGKMITGQTIPDAIAALGS
                     LNIIAGELDR"
     gene            complement(1983..2564)
                     /gene="nuoB"
                     /locus_tag="VEL74_11135"
     CDS             complement(1983..2564)
                     /gene="nuoB"
                     /locus_tag="VEL74_11135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HYG63126.1"
                     /translation="MSSTIIGESWVTTKFENLEEWAQRHSFWPFPFGTACCAIEFMST
                     VSSHYDVSRFGSEVVRFSPRQSDVLLVAGTINDKMAPVLKKIYDQMADPKWVISMGAC
                     ACSGGFYRAYHVMQGIDEIIPVDVYVPGCPPTPEGLLYGILQLREKVVRDRRKHGREY
                     PEPALASSEGKAEVEDQSRKESRESAHTGAATA"
     gene            complement(2780..3223)
                     /locus_tag="VEL74_11140"
     CDS             complement(2780..3223)
                     /locus_tag="VEL74_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63127.1"
                     /translation="MSRIPRFQEHLKTGFTAEAASAARCRAFAVQAERDGLPNLAKNW
                     LRMAAEKDKLAIQLLDASEQVRGMDADLGTAIAEERYENDVLYPKMVRDVDQDTADVL
                     LKVVNAQKEHLRQLESLRVSLNAAQGDVQLPAEVDRGTAPSAPAS"
     gene            3406..>4841
                     /locus_tag="VEL74_11145"
     CDS             3406..>4841
                     /locus_tag="VEL74_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HYG63128.1"
                     /translation="MDKALSRRAILLWAAALAAALFLALSLLSIPFVSVFAQVMLAGL
                     GLLVFLWLLVGFYRAFLWKVGRRLAFSYFLIGVLPIPMVLLLLTVVSYLLAGFFLGHL
                     YRDAVEEVQVELEQAVRVRAEAFARTGAVPEEPAPENIIFGYYRNGRRVGGDPSMPAT
                     MPAAWPAGIRSEGRRGEIADGEPALRFYPARNGDPTLAASAGEAGRGVVALYAGDLSG
                     EVSRRAGLWVEIEKPGELDLEVVNLAIGEHRIPLQRLGGMGRGADAEAEKFMKRLSKG
                     ERFWDDSLLSWVEVSGPLIDPGSGRMLSEYVPAVLSGTPRIVASHLFTGSSVDTATWG
                     ALLVLSLLLLNVYGVAVLMAVFMIFGLSYAVNRLSRATEAVRQGDFSVRIPVRRKDQV
                     GDLQRSFNQMAGDLESLVATATQKELLEKELSLARDLQKSLLPSDLPSGHGAAIAALF
                     EPSAAIGGDYFDVLRISEGVLAVFIADVS"
BASE COUNT          705 a         1597 c         1673 g          866 t
ORIGIN      
        1 ctccgggtag gagatgatcg gcagcttctt gtagttgaag aggttcttcg tgaggtgcct
       61 gagggtgcgg aacatgccgc cgatgacctg cggcaggtag aggcggtcga gggccgacag
      121 ctcctgccgc tcgaccttga ccacaccttc ggagacgcgc gttacggtcg tcatgcaatc
      181 tgtctcgttt caacgatgaa cgatgaggga tgaacgatga accagcggga tcgacggcgt
      241 gcttcgttcc tcgttccgtg ttccgcgttc cgcgttccgg tcaccggtcg agctccccgg
      301 cgatgatgtt cagcgagccg agcgcggcga tcgcgtcggg aatggtctgt ccggtgatca
      361 tttttccgaa tgcctcgaag atcgtgaagc aggggggcct ggcgcggacg cggtacgggt
      421 gtttcgtgcc gtccgacacg atgtagaagc cgacctcccc gttgccaccc tcgacctgga
      481 agtaggtctc gccgaccggc ggctggatgc cgtgcatgat cagcttgaag tggtagatga
      541 gcgactccat ctcgttgtag accttctcct tcgggggaag ggccacgtac gggttgtcga
      601 ccatgaccgg gccgggcggc agcccgcggt cgatggcctg ctggaggatg cggatcgact
      661 ggcgcatctc ctcgaagcgc accaggaagc gggcgtaggt gtcgcctccc tcggccaccg
      721 ggacgtccca gtcgtaggtg tcgtagccga ggtagggaga ggccttgcgg acgtcgaacg
      781 gcacgccggt ggcgcgcagg cacggtccgg tgaagccgaa gttgatcgca tcctcgcgcg
      841 agatcacgcc gacgccgtcg acgcggtcgc ggaagatgcg gttcttcatg atcagcgcct
      901 cgacgtcgtc gatgtacttc ggcagcgtgt tgacgaggtg ctgcgcgtgc gtgaggaacg
      961 tcgacgggac gtcgatggcg atgccgccga cgcggaggta gctcggcagc aggcgcgcgc
     1021 cggagcacga ctcggccagg ccgatgatct cctcgcgcgg ctggaagagc caccagaagt
     1081 tcgtcagcgc ccccatgtcg acgaggttcg tgccgatgca gatgatgtgg tcggagatgc
     1141 ggtagaactc cgacagcatc agccggacgt gctgcgcgcg gggaggcgcc tcgatgccga
     1201 ggagcttctc gaccgtcatg cagtagccga cgttgttgat gatggccgag cagtagttca
     1261 gccggtcggt gtacgggatg acctgctggt aggtgtgcgt ctccgacatc ttctcgaagc
     1321 agcggtgcag gtagccgatc tcgacgtccg aggcgacgat ggtctcgccg tcgagcaccg
     1381 ccatcagccg gaaggtgccg tgcatcgccg ggtgggacgg gccgatgttg accgtcatcc
     1441 gctcgaagag gtcgtcctcc ccctccggca ggtcgaagtg gggcttgtag atcttgtcct
     1501 cggtcaggat ccagcgcttg gtcgggtggt agtccttgcg cagggcgtgg ccgacgaacc
     1561 cctcgtggca caggatgcgc cgcaggttcg ggtgcccctc gacccggatg ccgaacatgt
     1621 cgtacagctc gcgctcgaac cagttgcacc ccttccacac cgggatgagc gacggcagca
     1681 cggcgtcgtc ctcgcgcagc ggcaccttca ggcgcaggcg cgtgttgcgt tggatcgaat
     1741 agagatggta gatggcctcg aagcgctcct cccggtcggg gaagtcgacg cctccgaggt
     1801 cgagcatcag gtcgtaccgc gtcgccgggt tgtgcttgag ctcccgggcg aggtcggaga
     1861 ggacctcgcg gcggacgatc caggtctcct ggtcgagacc ctcctgccgg gtgatcatcc
     1921 ccgggaactt cgcgcggacc gcctcggccg cggcatcccc gttgccggcc gtcgtcgtcc
     1981 ggtcaggcag tggcggcgcc agtgtgggca ctttcgcggc tctcctttcg gctctggtct
     2041 tctacctcgg ccttcccctc tgaggaggcc agggcgggct ccgggtactc ccggccgtgc
     2101 ttgcggcggt cgcggacgac cttctcgcgg agctggagaa tgccgtacag cagcccctcc
     2161 ggcgtcggcg ggcagccggg aacgtagacg tccaccggga tgatctcgtc gataccctgc
     2221 atcacgtgat aggcgcggta gaagccgccc gagcaggcgc aggcccccat ggagatcacc
     2281 cacttgggat cggccatctg gtcgtagatc ttcttgagca ccggcgccat cttgtcgttg
     2341 atggtgcccg ccaccagcag cacgtcgctc tgccgggggg agaagcgcac cacctccgag
     2401 ccgaagcgcg agacgtcgta gtggctcgac accgtcgaca tgaactcgat ggcgcagcac
     2461 gcggtcccga aggggaaggg ccagaacgag tgccgctgcg cccactcctc caggttctcg
     2521 aacttcgtcg tgacccagct ctcaccgatg atggttgatg acatggtctt atatcctcgc
     2581 gcgcgcagtc tatcacttgt gaccaccggt cacaagcgga acaagtagct ttgtttcctg
     2641 ttacttaacg gcgccggacc cgcttatcag atcatcgggt gggatctctg gggaggggcg
     2701 ccgggcaggg gacttccccg ctgactgcaa cgcggccggc cctcccgggc attccagctc
     2761 cgggagggcg gggacaggat caggaggccg gtgccgaggg ggcggtgccc cggtcgacct
     2821 cggcggggag ctggacgtcg ccctgggcgg cgttcaagga gacgcgcagc gactcgagct
     2881 ggcgcaggtg ctccttctgg gcgttgacga ccttgagaag gacgtcggcg gtgtcctggt
     2941 cgacgtcgcg caccatcttg ggatagagga cgtcgttctc gtacctctcc tcggcgatgg
     3001 cggttcccag gtcggcgtcc atgccgcgca cctgctccga cgcgtccaga agctggatcg
     3061 ccagcttgtc cttctcggcc gccatgcgca gccagttctt cgccaggttg ggcaggccgt
     3121 cccgttccgc ctggacggcg aaggcccggc aacgggcggc gctcgcggcc tcggcggtga
     3181 agccggtctt caggtgctcc tggaaacgcg gaatcctcga cacgatgtcc tctcctcctg
     3241 cttcacgcta agatgccccg gcgcgcaccg cccgccgggg agggagccgt cattctatgc
     3301 aactccatcc ggcgagccaa atggtggcac ccggtggaaa ctttgctccc tacctctcgt
     3361 agagctttcc ggggcccctg aaggcgcccc cggcagacac gccccgtgga caaagccctt
     3421 tcgcgcagag ccattctcct gtgggcggcc gccctggccg ccgcgctgtt cctggcgctg
     3481 tcgctcctgt cgatcccgtt cgtcagcgtc ttcgcccagg tcatgctggc ggggctgggc
     3541 ctcctggtct tcctctggct gctggtcggg ttctaccggg ccttcctctg gaaggtgggc
     3601 cggcgcctcg ccttctccta cttcctgatc ggcgtcctcc ccatcccgat ggtgctgctg
     3661 ctcctgacgg tggtctccta tctcctggcc ggcttcttcc tcggccacct ctaccgcgac
     3721 gccgtcgagg aggtccaggt ggagctggag caggcggtcc gcgtccgcgc cgaggccttc
     3781 gcccgcaccg gcgcggtccc cgaggagccg gccccggaga acatcatctt cggctactac
     3841 cggaacggcc gccgggtggg aggcgatccc tcgatgcctg caacaatgcc cgcggcctgg
     3901 cccgcgggga tccgctccga gggccggcgc ggcgaaatcg ccgacggcga gccggccctc
     3961 cgcttctacc ccgcgcgcaa cggcgacccg acgctcgcgg cgtccgccgg agaggcgggg
     4021 cggggggtgg tcgccctgta tgccggcgac ctgagcggcg aggtcagccg gcgggcgggc
     4081 ctctgggtgg agatcgagaa gccgggcgag ctcgacctgg aggtggtgaa cctggccatc
     4141 ggcgagcacc gcatccccct gcagcggctg ggcggcatgg gccgcggcgc cgacgcggag
     4201 gccgagaagt tcatgaagcg gctctccaag ggggagcggt tctgggacga ctccctgctc
     4261 tcctgggtgg aggtctccgg cccgctcatc gatcccggca gcggcagaat gctctccgag
     4321 tacgtgccgg cggtcctctc cggcacgccg cgcatcgtcg ccagccacct cttcaccggc
     4381 tcgtcggtgg acaccgccac ctggggggcg ctcctggtgc tcagcctgct gctcctgaac
     4441 gtctacggcg tggcggtgct gatggcggtg ttcatgatct tcggcctgtc ctacgcggtg
     4501 aaccggctga gccgggccac cgaagcggtc cgccaggggg acttctcggt ccgcatcccg
     4561 gtgcggcgca aggaccaggt cggggacctc cagcgctcgt tcaaccagat ggccggcgac
     4621 ctcgagtccc tggtggcgac cgcgacccag aaggagctgc tggagaagga gctgtccctg
     4681 gcgcgggacc tccagaagag cctcctccct tcggacctcc cgtcgggcca cggcgccgcc
     4741 atcgccgccc tcttcgagcc cagcgccgcc atcggcggcg actacttcga cgtgctgcgg
     4801 atctcggagg gggtgctggc ggtcttcatc gccgacgtct c
//
LOCUS       DASPJI010000414        15759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_769965, whole genome shotgun sequence.
ACCESSION   DASPJI010000414 DASPJI010000000
VERSION     DASPJI010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15759)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15759
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769965"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /locus_tag="VEL74_11150"
     CDS             complement(<1..1451)
                     /locus_tag="VEL74_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HYG63129.1"
                     /translation="MSETAGQHEEEVLGKAYDSRLMRRLLAYLRPYRWMTAGAVGLIL
                     LGSLFQLVGPLAMAVALDLFIQPQGAEGEVSAVSRQIGELLLARGIDPAAVRLEGVAA
                     VSLVYLASLILTFVLLFAQGYVMQLIGQYIMRDLRQEIFGHLQRLPISFYDRNPIGRL
                     VTRVTTDVDALNEMFSSGIVAIFGDILMLSGIVGVLFWLDWRLALVTFSIVPLLLLLT
                     FWFKRRVRDSFRDVRVRIARINSFLQEHITGMAVVQLFNREQGVYRDFQRINDEHREA
                     NVRAIFYYAVFYPGVELITALGLGLILWFGGGEVISGAVSIGALIAFLQYAQRFYQPL
                     ADLSDKYNILQGAMASSERVFKLLDTPVTITSPAEAYSPPEIRGAIEFDDVWFSYKEN
                     EPVLKGISFRVEPGKTLAVVGHTGAGKSTLANLLLRFYDISQGTVRVDGVDVREWDLP
                     RLRKSIAMVLQDVFLFSGTVGANIRLGEEEIDL"
     gene            1575..2690
                     /locus_tag="VEL74_11155"
     CDS             1575..2690
                     /locus_tag="VEL74_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013942228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HYG63130.1"
                     /translation="MPPTLNQLLKAMVDQGGSDLHVTTNTPPQVRVDGVLKALNLPPM
                     TPSETKQLAYSILTDNQKHRLEENLEIDLSFGIKGLARFRANIYHQRGAIAAAFRQIP
                     YEIRTFRELGLPATIEKLCEKPRGLLLVTGPTGSGKSTTLAAMIDKVNRERAEHIVTI
                     EDPVEYLHSHKKCIVNQRELHADTHSFANALRSALRQDPDVVLIGEMRDLETVEAALR
                     IAETGHLTLATLHTNSAAQTINRIIDVFPAHQQGQIRVQLSFVLEGIVCQSLLPKAGG
                     KGRALAMEVLVPTAAIRNLIREDKVHQIYGMMQAGQAKHGMQTFNQSLAALYFKRLIS
                     LQTALARSSYPDELQEIINRGPASLPTMGVGPEEARS"
     gene            2694..3899
                     /locus_tag="VEL74_11160"
     CDS             2694..3899
                     /locus_tag="VEL74_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HYG63131.1"
                     /translation="MPSYIWKGKTRAGQLQEGVLLADSRDAAAAVLRRQQIQVTAIRE
                     KGREVKLLPRLPQSIGQKRIAIFTRQFSVMLDAGLPLVQCLEILGEQEENRTFQTILQ
                     QVRGDVEQGMSLADSMRKHPKAFNDLFVNMIAAGEAGGILDIILQRLSSYIEKAVKLN
                     SQVKSALIYPVAVIVIAALVVFIILWKVIPVFAQLFAGLGGEMPFLTRMVIGASNFVA
                     RFFPFILLLVVLTYMAITRYHKTHRGRRVLDGLLLKVPVIGMLLRKIAVARFCRTLAT
                     LTSSGVPILEGLEITAKTSGNAIIEDSIMSVRKSVEEGRTISEPLAQTKVFPAMVVQM
                     INVGEQTGALDQMLSKIADFYEDEVDAAVAGMMKLIEPLLISFLGVVIGTIVAAMYLP
                     LYSILTKIG"
     gene            3988..5688
                     /locus_tag="VEL74_11165"
     CDS             3988..5688
                     /locus_tag="VEL74_11165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG63132.1"
                     /translation="MYPLSRQLQWYIALRVVVVVTVLLAYSFLGVYPETTGEVGFRIW
                     LYLGLPCFAATLLYIVLLRLLRGNPGVHAYIQFFGDLLLVTALVYSDAGGGIPSPFST
                     LYLIIIAVAATLLRRRAGVIVATAALVLYALPALFPRLHLLLRPGDGYVLLPFGPDLF
                     NNLVVHGFGFYAMAFLTSYLAHNITRAERELEEKSEHLADLQVAHRDVIQSIGSGLIT
                     TDLDGVITSVNQAGLAILERPETGLVGTPIQESGLFSAERWGEMTAASEQRRKLRSEV
                     DLERGGDIRPVGFSISQLTDAEGTQRGYIVVFQDLSRWRRMQEELRMKDRMAAVGELA
                     AGLAHEIGNPLAAISGSVQMLSSSLQGDPAQRKLLEILLKESQRLDRTIKGFLRFARP
                     RERSSVAFDVARLLAENFELLKNSEEVLPGHQLELALDPPSARLLADPDQMSQIFWNV
                     TRNSLRAMPGGGTLRVVGRLTQDTYCIEFMDTGRGMSEEQRANLFHPFQSFFDGGTGI
                     GMAIVYRIVQDHGGRLHVDSRPGVGTTISVELPADAPGTPGADEPLSQTLSAVPAEVP
                     "
     gene            5688..7073
                     /locus_tag="VEL74_11170"
     CDS             5688..7073
                     /locus_tag="VEL74_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYG63133.1"
                     /translation="MAARLLIVDDEQSLVEFLSLLFLGEGYEVDTASSAEEARRALEG
                     RSFDLVLCDILMPDGNGLDLLKEIKAANGNTAVIMMTAYTSTKSAIEAMKLGAYDYIS
                     KPFDVEELKVLAQKALERAELADENIYLRRELEQKYTFNNIIGKSPRMQAIFSMVERV
                     ARTASTVLVHGESGTGKELIARAIHFASPRAGRRFLSINCGALPENLLESELFGHERG
                     AFTGAVRDKKGLFQEADRGTLFLDEIGEMTPTMQVKLLRALQEKVVRKVGGTEEESVD
                     VRIIAATNQDLESRISTGDFREDLYYRINVLPIHLPPLRQRREDIPLLVEFFLQKYCR
                     QMELPPRRISVEAMEMLESYDWPGNVRELENVIERTLALSTSETITTRDLPVHLLTHR
                     KASSDMIHLPAEGMDLEAYLEGIRAQLMAEALERTGGVQTQAAELLNMSFRSFRYYAK
                     KAGLKGGDNGE"
     gene            complement(7088..8944)
                     /locus_tag="VEL74_11175"
     CDS             complement(7088..8944)
                     /locus_tag="VEL74_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63134.1"
                     /translation="MRNRTLSRPAVAVALFLLILAAAASAKEWRPAPLWGADVRSLAI
                     HPEDPDLVLAGTSAGQVYLSKNGGKRWSDAGPALPFPGWVVSALRFDPNRPSRIWASL
                     WGIWGSGHVAFSDDLGKSWVSRAKGLPDEPVYSLALVPGREGRLYAGTLSGVYGSTDD
                     GESWRYLTGDLPEIQKVTSLLVDTEAGTVIAGTWRRAYRSGDDGKTWAGIFDGMVLDS
                     EVFSLTPIPGRPGEIWATTCGWVYQSKNGGDKWERFKDGFTERRTPSFAALPDGRLLA
                     GTVAGLHVSEDGKNWKLAGDPALAIHTIAHHPARPGRVILATEGSGVWISEDGAANFR
                     RSSAGMTNTRVSALALSGDDVIVALSHAGPLSGAYVSKDRGRTWAAEFAPLPTVLELA
                     ASSGRVYAATERGLYERRGSGWFRLKELGEGRVEQVAIHGERVVARTAGALYELAGGR
                     FVPRPFKEGTPRSIAFFGDALWVSDGKGLYQLLEKANTPVAAPPAPMSGGRLQRLQDQ
                     LLLWGPGGAWLRGGPDSLWVELTGKPSRVLPTGDPRFPALMVSGETARLYDREARKFR
                     ILEVPVPARDVSAALVVDGKLLMGTSGYGLLIHELAPAVEKPVAVAAGGGTE"
     gene            complement(8937..9782)
                     /locus_tag="VEL74_11180"
     CDS             complement(8937..9782)
                     /locus_tag="VEL74_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HYG63135.1"
                     /translation="MSTIPSVPEVLAEAERSLQFHGRVVLCQIVRAEGSTPGKVGWKL
                     LARPDGSAYGNLGGGAFEALVKVDALAKLNGTPAGSEVKRYYFTEDAVKGEPTGMVCG
                     GMAEVFLDVVKAAPLLVICGGGPVGQALARAGSLAGFEILLAEDREEFRDPALFPAGT
                     RLAGVGRDYEEDFLAPVRGRDLHVAVVSRCWETDVAALASVLRQELPGLRYLGLMGSR
                     RKIARVRQEVADLGFDLSGVRLRAPIGLPIGGDSPGEIAISILAEVIQTRYERDEKVE
                     MSKDA"
     gene            complement(9790..12279)
                     /locus_tag="VEL74_11185"
     CDS             complement(9790..12279)
                     /locus_tag="VEL74_11185"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HYG63136.1"
                     /translation="MSGWSPADLLPGLYVLALGGLLALALRRWYDAVPRRVWAVFAVL
                     LVVLFGRVLFGGGVLLPLDNLRINIPFSHLPPPEPRGIAMQGDLVHQIAPWLLEVRRA
                     VSEGRWPLWSSRTGAGMPLLGDPQAQVLQPLVLLAYPFDLWSAVGITAALRVLLALVF
                     LFLLLRRQGLGEPAALCGSLAFGLGGFLMLWLGWPMANSAALLPLALYAVVRCDQSGR
                     TRDHLLLTLAAASLLLGGHPETFVYALAFTGLFLLARCLERWRQDRAAALALLRRCAL
                     SLILAGFLVAPVLLPIQDYLPKTNRGAVVAYQLSFKPLPELWRELQKPETLERWRERA
                     EKRLVPVAAPRAFGRFDFPYWGLDNLIEDASGFAGSATLLLALLSLLSLFGKDRLPQE
                     RLAGLTLLAGLALIAQPPGFERLAGQLPVIGATAIHRHHRILLLVCFCLAYLAACEVE
                     RRRRGEGKRWPVIGAMAVVAALIAWGYLGHPHPEHPRMIIDEQMRWLALQLGAVALAA
                     ATLLLAGRRRWLPWGPLPWAIAAVVAIELLAVHLPAVPPAPRRLAYPVTPPIRFLLDH
                     LHGTDGRMVGVGQAFLANFPAVYGLNDARIDNPSLPDGYAHVTWTLRRQFLAPVFSRP
                     SHPLYDLLGVRYVIVRPGVKMPLPLVFQSPAGWIYQRPRALPRLFLPASAEVFQGGLW
                     GTWLERNRDFAARALVQPSPEQRRQTWRSRAPGSSNVVLESLEPQHLRARALLTERRL
                     LASSVYQDGHWVLLANGEPAPRLLANGPFVAAWLPPGDWRLDLLYRPPVFIAGCLLAA
                     LALTAAAARWVPPPSLSRRAGAAGGRPRVLS"
     gene            complement(12280..13623)
                     /locus_tag="VEL74_11190"
     CDS             complement(12280..13623)
                     /locus_tag="VEL74_11190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG63137.1"
                     /translation="MTLAFPSDPVSPFVAALMSELRREVPGMAGVDLSVDERDEMLGF
                     LTSLHGGDRDRALFAYFQSGWSIARSMEQVLRWRFGSERPGRLLDFASGYGRVTRFLL
                     RDLPPERVWVSDLYADAVRFQEERFGVHGLVSTVRPEDFACSERFDVILVTSLFTHLR
                     EDRFVAWLAALCRLLAPGGLLAWSVHDESVLTPPLQMPAGGIRFEAVSESGSIEGSDY
                     GSAWVTEAFVRRSLEKVASGASVHRLERALCNYQDLYVAVLEPEVDFSGLAFRGEPQL
                     FLEGAALPEPGVLEVWGWAALRGGSLREVQVVLDGEVLAAGPVAGPRPEVAAMLGAGP
                     DLRPGWRLACPLPPALSRATGILMLRVVDDQGSPFPVWAGTAESLLLRSSRNEAGILE
                     RELARARDHIARLEPEAVRAGDLDARIAAMRASRFWKMRDAWFRVKRSLGLTDEE"
     gene            13841..14185
                     /locus_tag="VEL74_11195"
     CDS             13841..14185
                     /locus_tag="VEL74_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63138.1"
                     /translation="MKLKLLLSLALVTLTAGSALAAEPVPVAPESAVTSAAAAEEVAL
                     PDELLAQLTPEPTDKIGWKQGPCTVSVTCAPGYVVRCTGQDSCSWKGHSSWSNPGYVE
                     CDGFRTTCTILP"
     gene            14679..15026
                     /locus_tag="VEL74_11200"
     CDS             14679..15026
                     /locus_tag="VEL74_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63139.1"
                     /translation="MKLKLLLSLALIILTAGSALAAEPVTVAPESAVTSDAAADEVAL
                     PDELAAQLTPEPTYKIGVKHGPCSVSVTCAPGWVISCSGQSICYWKVHSSWSNPGYVE
                     CDGRRTTCLLTPE"
     gene            15265..>15759
                     /locus_tag="VEL74_11205"
     CDS             15265..>15759
                     /locus_tag="VEL74_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63140.1"
                     /translation="MRKLPLLLAVFATALVLPAAAHAYCTGVAPGSPGHVCGSIYKLG
                     ASGWKAAGPTNVLLCPRGSRTGCRGTTTITLNDGWGRPYQNFTIPNFSQYYGMTTYRD
                     FDIYLWSASSTDYWGSQYAVHGYTTIGPYGLDGLSWAMAPRPLPPTPVYPTGTSVPSS
                     YTVRW"
BASE COUNT         2562 a         5524 c         5510 g         2163 t
ORIGIN      
        1 tcgaggtcga tctcctcctc ccccaggcgg atgttggccc ccaccgtgcc ggagaagagg
       61 aagacgtcct gcagcaccat ggcgatgctc ttgcgcagcc gcggcaggtc ccactcgcgg
      121 acgtcgacgc cgtcgacgcg caccgtcccc tgggagatgt cgtagaagcg cagcagcagg
      181 ttggcgaggg tgctcttgcc ggcgccggtg tggccgacca cggcgagcgt cttgccgggc
      241 tcgacgcgga acgagatccc cttgagcacc ggctcgttct ccttgtagga gaaccagacg
      301 tcgtcgaact cgatggcgcc gcggatctcc ggcggcgagt aggcctcagc cggcgaggtg
      361 atggtcaccg gcgtgtcgag gagcttgaac acccgctccg agctcgccat cgccccctgc
      421 aggatgttgt acttgtccga caggtcggcg agcggctggt agaagcgctg ggcgtactgg
      481 aggaaggcga tcagcgcgcc gatcgacacc gcgccggaga tcacctcgcc gccgccgaac
      541 cagaggatca gcccgagccc caaggcggtg atcagctcaa cgccggggta gaagaccgcg
      601 tagtagaaga tggcgcggac gttcgcctcg cggtgctcgt cgttgatgcg ctggaagtcg
      661 cggtagaccc cctgctcccg gttgaagagc tgcaccacgg ccatgccggt gatgtgctcc
      721 tggaggaagg agttgatgcg cgcgatccgg acccgcacgt cgcggaagct gtcgcgcacc
      781 cggcgcttga accagaaggt caggagcagc agcagcggca cgatcgagaa ggtgaccagc
      841 gccagccgcc agtcgagcca gaagagcacg ccgacgatgc cggagagcat caggatgtcg
      901 ccgaagatcg ccacgatgcc cgacgagaac atctcgttca gggcgtcgac gtcggtcgtg
      961 acccgggtga ccagccggcc gatggggttg cggtcgtaga aggagatggg cagccgctgg
     1021 aggtggccga agatctcctg ccgcaggtcg cgcatgatgt actgcccgat gagctgcatc
     1081 acgtaaccct gggcgaagag gaggacgaag gtgaggatca ggctcgccag gtagaccagg
     1141 gagacggccg cgaccccctc cagccgcacc gccgccgggt cgatcccgcg ggcgaggagc
     1201 agctccccga tctgccggct gaccgccgac acctccccct ccgcgccctg gggctggatg
     1261 aagaggtcga gcgccaccgc catggccagg gggccgacga gctggaacag cgacccgagc
     1321 aggatcagcc ccaccgcgcc cgccgtcatc caccggtagg ggcgcaggta ggccaggagg
     1381 cgccgcatca ggcgggagtc gtaggctttg ccgaggacct cctcctcgtg ctgtccggcc
     1441 gtctcgctca cgggggtacg atatcaaacc gcctccagcc acggttcggg actgcttatg
     1501 tcttttcctc tataccctaa tggtgtagac tgcgctccag ctctcgtagc cagacttctt
     1561 taggggtatg tcgcatgccg ccgacgctga accagcttct gaaggctatg gtcgatcagg
     1621 ggggatcgga cctgcacgtc accaccaaca cccccccgca ggtccgggtg gacggtgtcc
     1681 tcaaggcgct gaacctgccg ccgatgacgc cgtcggagac caagcagctc gcctactcga
     1741 tcctgaccga caaccagaag caccggttgg aggagaacct ggagatcgac ctctccttcg
     1801 ggatcaaggg gctggcccgc ttccgggcca acatctacca ccagcggggc gccatcgccg
     1861 cggccttccg gcagatcccg tacgagatcc gcacgttccg cgagctcggg ctgccggcga
     1921 cgatcgagaa gctgtgcgag aagccgcgcg gcctgctcct ggtgaccggg cccaccggct
     1981 cgggcaagtc gacgaccctc gccgccatga tcgacaaggt caaccgcgag cgcgccgagc
     2041 acatcgtcac catcgaggac ccggtggagt acctgcacag ccacaagaag tgcatcgtca
     2101 accagcggga gctgcacgcc gacacccaca gcttcgccaa cgccctgcgc tcggcgctgc
     2161 ggcaggaccc ggacgtggtg ctgatcggtg agatgcgcga cctcgagacg gtggaggcgg
     2221 cgctgcgcat cgccgagacc ggccacttga cgctcgcgac gctgcacacc aactcggcgg
     2281 cgcagacgat caaccgcatc atcgacgtct tccccgccca tcagcagggg cagatccggg
     2341 tgcagctctc cttcgtgctg gaggggatcg tctgccagtc gctgctcccc aaggcggggg
     2401 ggaaggggcg ggcgctggcg atggaggtgc tggtccccac cgcggcgatc cgcaacctga
     2461 tccgcgagga caaggtgcac cagatctacg gcatgatgca ggccggccag gccaagcacg
     2521 gcatgcagac gttcaaccag tcgttggccg cgctgtactt caagcggctg atctcgctgc
     2581 agacggccct cgcccgctcc tcgtacccgg acgagctgca ggagatcatc aatcgcggcc
     2641 ccgcgagcct ccccacgatg ggggtcgggc cggaggaggc aaggagttag accatgccca
     2701 gctacatttg gaaaggcaag acccgagccg gtcagctcca ggagggcgtg ctgctggccg
     2761 actcccgcga cgccgcggcg gcggtcctga gacggcagca gatccaggtc accgcgatcc
     2821 gcgagaaggg gagggaggtc aagctcctgc cccgcctgcc gcagagcatc ggccagaagc
     2881 ggatcgccat cttcacccgc cagttctcgg tcatgctgga cgccggcctg ccgctcgtcc
     2941 agtgcctcga gatcctcggc gagcaggagg agaaccggac cttccagacc atcctccagc
     3001 aggtgcgggg cgacgtcgag cagggcatgt cgctggccga ctcgatgcgc aagcacccca
     3061 aggccttcaa cgacctgttc gtcaacatga tcgccgccgg cgaggcgggc ggtatcctcg
     3121 acatcatcct gcagcgcctc tcgagctaca tcgagaaggc ggtcaagctg aacagccagg
     3181 tcaagtcggc gctgatctac ccggtggcgg tcatcgtcat cgccgccctg gtggtgttca
     3241 tcatcctgtg gaaggtcatc ccggtgttcg cccagctctt cgcgggcctg ggcggcgaga
     3301 tgcccttcct cacccgcatg gtgatcgggg cgagcaactt cgtggcgcgg ttcttcccgt
     3361 tcatcctgct gctggtggtc ctcacctaca tggcgatcac ccgctaccac aagacccacc
     3421 gcggccggcg ggtgctcgac gggctgctgc tcaaggtgcc ggtcatcggc atgctgctgc
     3481 gcaagatcgc cgtcgcccgc ttctgccgga cgctcgccac cctgacctcc tcgggcgtgc
     3541 cgatcctcga aggcctggag atcaccgcca agacctcggg caacgcgatc atcgaggact
     3601 cgatcatgtc ggtccgcaag agcgtcgagg agggccgcac catctccgag ccgctcgccc
     3661 agaccaaggt cttcccggcc atggtcgtgc agatgatcaa cgtcggcgag cagacgggcg
     3721 ccctcgacca gatgctctcc aagatcgccg acttctacga ggacgaggtg gacgccgccg
     3781 tcgccggcat gatgaagctg atcgagccgc tcctgatctc gttcctcggc gtggtcatcg
     3841 gcacgatcgt cgcggcgatg tacctgccgc tctactcgat cctgaccaag atcggctgac
     3901 ggccgcggac tctctacaat agccggaggc ggtgacccgc cgcctccggc tctcatgtcc
     3961 cagacggcac caccctcgcg agccccgatg tacccgctct cccgccagct ccagtggtac
     4021 atcgccctgc gcgtggtggt ggtcgtcacc gtcctcctcg cctactcgtt cctcggcgtc
     4081 tacccggaga ccaccggcga ggtggggttc cgcatctggc tctacctggg gctgccgtgc
     4141 ttcgcggcga ccctcctcta catcgtcctg ctgcggctgc tccgcggcaa cccgggcgtc
     4201 cacgcctaca tccagttctt cggcgacctg ctgctggtca ccgcgctggt ctactcggac
     4261 gcgggcggcg gcatcccgag cccgttctcg accctctacc tgatcatcat cgcggtggcc
     4321 gcgacgctgc tgcgccgccg cgccggcgtc atcgtcgcca cggcggcgct ggtgctctac
     4381 gcgctgccgg ccctgttccc gcgcctccat ctgctgctgc gtcccggcga cggctacgtg
     4441 ctgctgccgt tcgggcctga cctgttcaac aacctggtgg tccacggctt cggcttctat
     4501 gccatggcct tcctcacctc ctacctggcg cacaacatca cccgcgccga gcgcgagctg
     4561 gaggagaaga gcgagcacct cgcggacctc caggtggcgc accgggacgt catccagtcg
     4621 atcggcagcg gcctcatcac caccgacctc gacggggtga tcaccagcgt caaccaggcc
     4681 ggcctggcga tcctggagcg gcccgagacc ggtctggtgg ggacgccgat ccaggagtcg
     4741 ggcctgttct cggccgaacg ctggggggag atgaccgccg ccagcgagca gcgcaggaag
     4801 ctgcgcagcg aggtggacct cgagcgcggc ggcgacatcc ggccggtcgg cttctcgatc
     4861 tcgcagctca ccgacgccga ggggacgcag cgcggctaca tcgtggtctt ccaggatctg
     4921 tcgcgctggc ggcggatgca ggaggagctg cggatgaagg accggatggc ggcggtgggc
     4981 gagctcgcgg ccggcttggc gcacgagatc ggcaacccac tggcggcgat ctccggctcg
     5041 gtccagatgc tctcctcgtc gctccagggg gaccccgcgc agagaaagct gctggagatc
     5101 ctgctcaagg agagccagcg cctggaccgc acgatcaagg ggttcctgcg cttcgcccgc
     5161 ccgcgggagc gctccagcgt ggcgttcgac gtcgcgcgcc tgctggccga gaacttcgag
     5221 ctgctcaaga acagcgagga ggtgctgccg gggcaccagc tcgagctcgc gctcgacccg
     5281 ccgtcggcgc gtctgctcgc cgatccggac cagatgagcc aaattttctg gaacgtcacg
     5341 cgcaactccc tgcgggcgat gccgggcggc ggcaccctga gggtagtggg gcggctgacg
     5401 caggacacct actgcatcga gttcatggat acgggacgcg gcatgtccga ggaacagaga
     5461 gcgaacctct tccacccctt ccagtccttc ttcgacggcg gcaccggcat cggcatggcc
     5521 atcgtctacc gcatcgtcca ggaccacggc ggccgcctgc acgtcgacag ccgtccgggg
     5581 gtgggcacca ccatcagcgt cgagctgccg gcggacgccc ccggcacccc gggagccgac
     5641 gagccgctgt cccagaccct ctcggccgta cccgcggagg tgccgtgatg gcggcgcgcc
     5701 tgctgatcgt cgacgacgag cagagcctgg tcgagttcct ctccctcctt ttcctgggcg
     5761 agggctacga ggtggacacc gcctcctcgg ccgaggaggc ccgccgtgcg ctcgaggggc
     5821 ggagcttcga cctggtgctg tgcgacatcc tgatgccgga cggcaacggc ctcgatctgc
     5881 tcaaggagat caaggcggcc aacggcaaca ccgcggtgat catgatgacc gcctacacct
     5941 ccaccaagtc ggccatcgag gcgatgaagc tgggggccta cgactacatc tcgaagccgt
     6001 tcgacgtcga ggagctcaag gtgctggcgc agaaggcgct cgagcgcgcc gagctggcgg
     6061 acgagaacat ctacctgcgg cgcgagctgg aacagaagta caccttcaac aacatcatcg
     6121 gcaagagccc gcgcatgcag gcgatcttct cgatggtcga gcgggtggcg cgcaccgcct
     6181 ccacggtgct ggtgcacggc gagagcggca ccggcaagga gctgatcgcg cgcgccatcc
     6241 acttcgccag cccgcgcgcc ggccgccgct tcctgtcgat caactgcggg gccctccccg
     6301 agaacctgct ggagagcgag ctgttcggcc acgagcgcgg cgccttcacc ggcgcggtgc
     6361 gcgacaagaa ggggctgttc caggaggcag accgggggac gctgttcctc gacgagatcg
     6421 gcgagatgac gccgacgatg caggtcaagc tcctgcgcgc cctgcaggag aaggtcgtcc
     6481 gcaaggtcgg cggcacggag gaggagtcgg tggacgtgcg gatcatcgcc gccaccaacc
     6541 aggacctgga gagccgcatc tccacggggg acttccggga ggacctctac taccggatca
     6601 acgtcctgcc catccacctg ccgccgctgc ggcagcggcg cgaggacatc ccgctgctgg
     6661 tcgagttctt cctgcagaag tattgccggc agatggagct gccgccgcgc cgcatctcgg
     6721 tcgaggcgat ggagatgctg gagagctacg actggccggg caacgtccgc gagctggaga
     6781 acgtgatcga gcggaccctg gcgctgtcaa cgtccgagac catcaccacg cgtgacctgc
     6841 cggtccacct gctcacccac cgcaaggcga gctcggacat gatccatctc ccggcggagg
     6901 ggatggatct cgaggcctac ctggagggga tccgcgccca gctcatggcc gaggcgctgg
     6961 agcgcaccgg cggcgtccag acccaggccg ccgagctgct gaacatgagc ttcagaagtt
     7021 tccgctacta cgcgaagaag gcgggcctca agggcgggga caacggggag taggggggcg
     7081 gcgcccccta ctcggtgcct ccccccgccg ccacggccac gggcttctcc accgccgggg
     7141 cgagctcgtg gatcagcagg ccgtagccgg aggtccccat gaggagcttg ccgtccacca
     7201 ccagggcggc ggagacgtcg cgggcgggca ccggcacctc gaggatccgg aacttcctgg
     7261 cctcgcggtc gtagaggcgg gcggtctcgc cgctcaccat cagggcgggg aagcggggat
     7321 cgccggtcgg gagcacccgc gacggcttgc cggtcagctc gacccagagc gagtcggggc
     7381 cgccgcgcag ccaggcgccg ccggggcccc agagcaggag ctggtcctgg aggcgctgca
     7441 gccggccgcc ggacatcggc gccggcggcg ccgctaccgg cgtgttcgcc ttctccagga
     7501 gctggtagag ccccttgccg tcgctcaccc agagggcgtc gccgaagaag gcgatcgagc
     7561 gcggcgtccc ctccttgaaa gggcggggga cgaagcggcc gccggccagc tcgtagagcg
     7621 ctccggcggt gcgcgccacc acccgctcgc cgtggatcgc gacctgctcg acccgcccct
     7681 cgccgagctc cttcaaccgg aaccagccgg agccccggcg ctcgtagagg ccgcgctcgg
     7741 tggcagcgta gacgcggccg gagctggcag cgagctccag caccgtcggc agcggcgcga
     7801 actccgccgc ccaggtgcgc ccgcggtcct tggacacgta ggcccccgac agcggcccgg
     7861 cgtggctcag cgccacgatc acgtcgtcgc cggagagggc gagggcggag acccgggtgt
     7921 tggtcatccc cgcgctggag cgccggaagt tggcggcgcc gtcctcggag atccagacgc
     7981 ccgagccctc ggtggcgagg atcacccgcc ccggccgggc cgggtggtgg gcgatggtgt
     8041 ggatggcgag cgccgggtcc cccgccagct tccagttctt gccgtcctcg gagacgtgga
     8101 ggccggcgac ggtgccggcg aggagccggc cgtcgggcag ggccgcgaag ctcggcgtgc
     8161 ggcgctcggt gaagccgtcc ttgaagcgct cccacttgtc gccgccgttc ttcgactggt
     8221 agacccagcc gcaggtggtg gcccagatct ccccggggcg gccggggatg ggggtcaggc
     8281 tgaacacctc ggagtcgagc accatgccgt cgaagatgcc ggcccaggtc ttgccgtcgt
     8341 cgccgctgcg gtaggcgcgg cgccaggtgc cggcgatgac cgtgcccgcc tcggtgtcga
     8401 ccagcaggct ggtgaccttc tggatttccg gcaggtcacc ggtgaggtag cgccagctct
     8461 cgccgtcgtc ggtcgagccg tagacgcccg agagggtgcc ggcgtagagg cgcccctcgc
     8521 ggccggggac cagcgccagc gagtagaccg gctcgtccgg cagccccttg gcgcgcgaca
     8581 cccagctctt gcccaggtcg tccgagaagg cgacgtggcc gctcccccag atcccccaga
     8641 gggaggccca gatgcgcgac gggcggttgg ggtcgaagcg cagggcgctc accacccagc
     8701 cggggaaggg gagggccggg ccggcgtcgg accagcgctt gccgccgttc ttcgacaggt
     8761 agacctggcc ggccgaggtg ccggcgagca ccaggtccgg gtcctcgggg tgaatcgcca
     8821 gggagcgcac gtcggcgccc cagagcggcg ccgggcgcca ctccttcgcg ctcgcggcgg
     8881 ccgccaggat caacagaaag agcgccaccg cgacggcggg gcgggaaagg gtccggttac
     8941 gcatccttcg acatctccac cttctcatcg cgctcatagc gcgtttgaat cacttcggcc
     9001 aggatcgaga tcgcgatctc gcccgggctg tcgccgccga tcggcaggcc gatcggcgcg
     9061 cgcagccgca ccccggacag atcgaagccg aggtccgcga cctcctggcg cacccgcgcg
     9121 atcttgcgcc ggctgcccat gagccccaga taccggagcc ccggcagctc ctggcgcagc
     9181 accgaagcca gcgccgccac gtcggtctcc cagcagcggc tgacgacggc gacgtggagg
     9241 tcgcggccgc ggaccggcgc caggaagtcc tcctcgtagt cgcgccccac cccggcgagg
     9301 cgggtcccgg cggggaagag cgccgggtcg cggaactcct cccggtcctc ggcgagcagg
     9361 atctcgaagc cggcgaggga gccggcccgc gccagcgcct gccccaccgg gccgccgccg
     9421 cagatcacga gcagcggcgc cgccttcacc acgtcgagga agacctcggc catgccgccg
     9481 cagaccatgc cggtgggctc ccccttcacc gcgtcctcgg tgaagtagta gcgtttcacc
     9541 tcggagccgg ccggcgtgcc gttcagcttc gccagggcat ccaccttgac cagggcctcg
     9601 aaggcgccgc cgcccaggtt gccgtaggcc gagccgtccg gccgcgccag cagcttccag
     9661 cccaccttgc cgggggtcga gccctcggcg cgcacgatct ggcacagcac caccctgcca
     9721 tggaactgca aagaccgttc cgcctccgcc aacacttcgg gcacggaggg aatcgtggac
     9781 ataccgggct caggatagca cccgggggcg tcccccggcc gctccggccc ttcgagataa
     9841 cgacggcggc ggcacccagc gggccgccgc ggcggtgagg gcgagcgccg cgagcagaca
     9901 gccggcgatg aagacgggcg gccggtagag gaggtcgagc cgccagtccc ccggcggcag
     9961 ccacgcggcg acgaacgggc cgttggcgag caggcgcgga gccggctcgc cgttcgccag
    10021 caggacccag tggccgtcct ggtagacgct ggaggcgagc agccgccgct cggtgaggag
    10081 cgcccgcgcc cgcaggtgct gcggctccag ggactccagg acgacattgg aggagcccgg
    10141 ggcgcgggag cgccaggtct ggcgccgctg ctccgggctc ggctggacga gggcgcgggc
    10201 ggcgaagtcg cggttgcgct cgagccacgt cccccagagc cctccctgga agacctccgc
    10261 cgacgcgggg aggaagaggc gcggcagcgc ccgcgggcgc tggtagatcc agccggccgg
    10321 gctctggaag accaggggga gcggcatctt cacgccgggg cggacgatga cgtagcgcac
    10381 cccgagcagg tcgtagagcg ggtggctggg ccgcgagaag accggcgcca ggaactggcg
    10441 gcggagcgtc caggtgacgt gcgcgtagcc gtcggggagc gacgggttgt cgatgcgcgc
    10501 gtcgttcagg ccgtagaccg ccgggaagtt ggcgaggaac gcctggccga cccctaccat
    10561 gcgcccgtcg gtcccgtgca ggtggtcgag gaggaagcgg atggggggcg tcaccggata
    10621 ggcgagccgg cgcggggccg gaggcacggc gggcaggtgc accgccagca gctcgatggc
    10681 gacgacggcg gctatcgccc agggaagcgg cccccagggg agccagcgcc gccggccggc
    10741 cagcagcagc gttgcggccg cgagggccac ggcgccgagc tggagggcga gccagcgcat
    10801 ctgctcgtcg atgatcatcc gcgggtgctc ggggtgcgga tgcccgagat agccccaggc
    10861 gatcagggcg gcgacaacgg ccatggctcc gatcaccggc cagcgcttcc cctccccccg
    10921 gcggcgacgc tccacctcgc aggcggcgag ataggccagg cagaagcaga cgagcagcag
    10981 gatgcggtgg tggcgatgga tggccgtggc gccgatcacc ggcaactgac cggccagccg
    11041 ctcgaagccc ggcggctggg cgatgagggc caggccggcg agcagtgtca ggccggcgag
    11101 gcgctcctgc ggcaggcgat cctttccgaa gagggagagc agggagagca gcgccagcag
    11161 cagggtggcg ctgccggcga agccgctcgc gtcctcgatg aggttgtcca gcccccaata
    11221 cgggaagtcg aagcggccga aggcccgcgg cgccgccacc ggcaccagcc gcttctccgc
    11281 ccgctcgcgc cagcgctcca acgtctccgg cttttgcagc tcgcgccaca gctccggcag
    11341 cggcttgaac gacagctggt aggccacgac cgccccccgg ttggtcttcg gcagatagtc
    11401 ctggatgggc agcagcaccg gcgccaccag gaagccggcc agaatcagag acagggcgca
    11461 gcggcggagg agggcgagcg ccgccgcccg gtcctgcctc caccgctcca ggcagcgggc
    11521 gaggaggaag aggccggtga aggcgagggc gtagacgaag gtctccgggt ggccgccgag
    11581 gaggagcgag gccgcggcga gcgtcaggag gaggtggtcg cgggtacggc cggactggtc
    11641 gcagcgcacc acggcgtaga gggccagggg gaggagcgcg gcggagttgg ccatcggcca
    11701 gcccagccag agcatgagga agccgccgag gccgaacgcc aggctgccgc agagggcggc
    11761 cggctctccc agcccctgcc gtcgcaggag aagaaagagg aagaccagcg ccaggaggac
    11821 ccgcaacgcc gcggtgatcc ccacggcgga ccagagatcg aagggatagg cgaggaggac
    11881 cagcggctgc agcacctggg cctgcgggtc gccgagcagc ggcatgccgg caccggtgcg
    11941 ggagctccag agcggccacc gcccttcgct cacggcccgg cgcacctcca gcagccaggg
    12001 ggcgatctgg tgcacgaggt cgccttgcat ggcgatgccg cgcggctccg gcggcgggag
    12061 gtggctgaac gggatgttga tccgcaggtt gtcgagcggc agcaggaccc cgccgccgaa
    12121 cagcacccgg ccaaagagca cgacgagcag cacggcgaac accgcccaca cccggcgcgg
    12181 cacggcgtcg taccagcgcc ggagcgccag cgccaggagg ccccccagcg cgaggacata
    12241 caggccgggg aggaggtccg ccgggctcca gccgctcatc tactcctcat ccgtgagccc
    12301 cagggagcgt ttgacccgga accaggcgtc gcgcatcttc cagaagcggc tcgcccgcat
    12361 ggccgcgatc cgggcgtcga ggtctccggc gcggaccgcc tcgggctcga gccgggcgat
    12421 gtgatccctg gcccgggcga gctcgcgttc gaggatgccc gcctcgttgc ggctggagcg
    12481 cagcaggagc gactcggccg tcccggccca gacggggaac ggcgaaccct ggtcgtcgac
    12541 cacccgcagc atcaggatgc cggtggcgcg ggagagggcg ggagggagcg ggcaggcgag
    12601 ccgccagccg ggccggaggt cggggccggc gccgagcatc gccgccacct cgggacgggg
    12661 acccgccacc gggccggcgg ccagcacctc gccgtcgagg accacctgga cctcgcggag
    12721 gctgccgccg cgcagggccg cccagcccca gacctccagc acgcccggct ccggcagggc
    12781 ggcgccttcg aggaagagct gcggctcgcc ccggaaggcg aggcccgaga aatccacctc
    12841 cggctccagg accgccacgt agaggtcctg atagttgcag agggcgcgct ccagccggtg
    12901 gacggaggcg cccgaggcca ccttctccag cgaccgccgg acgaacgcct cggtcaccca
    12961 ggcggacccg tagtcgctcc cctcgatcga gccgctctcg ctgaccgcct cgaagcggat
    13021 gccgcccgcg ggcatctgga gcggcggggt cagcacgctc tcgtcgtgga cgctccacgc
    13081 cagcaggccg cccggcgcga ggagccggca gagcgcggcc agccaggcga cgaagcggtc
    13141 ctcccgcagg tgggtgaaga gcgaggtgac gaggatcacg tcgaagcgct cggagcaggc
    13201 gaagtcctcg ggccgcaccg tcgagaccag cccgtggacg ccgaagcgct cctcctggaa
    13261 gcgcacggcg tcggcataga ggtcggaaac ccacacccgc tcgggcggca gatcgcggag
    13321 caggaagcgg gtgacccgcc cgtagccgct ggcgaagtcg aggagccggc cgggtcgctc
    13381 ggagccgaag cgccaccgca gcacctgctc catggagcgg gcgatgctcc acccggattg
    13441 gaagtaggcg aacagggcgc ggtcgcggtc cccgccgtgc agcgaggtga ggaagccgag
    13501 catctcgtcc cgctcgtcga cggacaggtc cacgccggcc atccccggaa cctcccgacg
    13561 gagctcggac atcagggcgg ccacgaaggg gctcaccgga tcggagggga aggccagtgt
    13621 catgacagaa ggagcatccg cgcgagacga cgcgggagtt tagcagggac gggagaggga
    13681 ggccgttccc tatctaataa ggacgcgttt ccggctcgat cagcttctcg ggtgacctcg
    13741 ccggcttccg gcctggattt ggaggctccc atgaggcggc gccgggcacc ctccctggta
    13801 gggcgactat ccgcccgcga atcagtaagg agagcgcacg atgaagttga agctcctgct
    13861 gtctttggcc ctcgtcaccc tgaccgccgg ctccgctctg gcggccgagc ccgttcccgt
    13921 ggcgccggag agcgccgtca cgtccgctgc cgccgccgaa gaggtcgcgc tgcctgacga
    13981 gctgctggcc cagctcaccc ccgagcccac cgacaagatc ggctggaagc agggcccctg
    14041 caccgtctcg gtcacctgcg ctcccggcta tgtggtccgt tgcacggggc aggactcctg
    14101 ctcctggaag gggcacagct cctggtccaa ccccggctac gtggagtgcg acggcttccg
    14161 caccacctgc accatccttc cctagtagat acgctccagt tggttcggct gacctcaagt
    14221 cgcctccgct aagcctattt cgggcccgat aagcgcatct ggcccgaatc aagcttggtg
    14281 ggctcggact gagcctggct gccgcgagct ggcggtagcg ggcccgagac aagcttggct
    14341 ggaaccggat agcgttagca ggcctgactc gagctcaact ggaacgagat gacgttagca
    14401 ggggcgagcc aagcccatct ggcgcgagac aaccttggcg gagacgggat aagcctatct
    14461 ggaacggctt ggcgttagcg ggcgcgacgt aagcttatcc ggcacgggcc aagctcaggt
    14521 cagatggacc cgagcttggc tgagacgttt cgctcttccg gcgcgcagaa gcctgtcgat
    14581 gtcagccgat cttggcgccg ccggctccca gggagcggcg ccgggcaccc tctctgtagg
    14641 gcgactatcc gcccgcgaat cagtaaggag agcgcacgat gaagttgaag ctcctgctgt
    14701 ctttggccct catcatcctg accgccggct cagctctggc ggccgagccc gttaccgtgg
    14761 cgccggagag cgccgtcacc tccgatgccg ccgccgacga ggtggcgttg ccggacgaac
    14821 tggccgccca gctcaccccc gagcccacct acaagatcgg ggtcaagcac ggcccctgct
    14881 cggtctcggt cacctgtgct cccggctggg tcatcagttg ctcgggccag agcatctgct
    14941 actggaaggt gcacagctcc tggtcgaacc ccggctacgt cgagtgtgac ggccgccgca
    15001 ccacctgcct cctcaccccc gagtagccgc cgtggtcccg gggcgcggct ccgtcgcccc
    15061 gggacgcccg tgctttccac ctgccggtcg gcctcgccgc cccggggctg ctgtccacgc
    15121 cgtttggcgc cggccgagcc atggacgacg gttctgcccc cgggatcgcg catggttggt
    15181 caagggaccc tcggaaggat tcgacagcac gccggcggtg gtcgccggcc ctccgcggtt
    15241 ccgcttcgac caaggagcgt gtccatgaga aagctacctc tcctgctcgc agtcttcgcc
    15301 actgcgctcg tcctccccgc cgccgcccac gcctactgca ccggcgttgc cccgggatca
    15361 cccggccacg tgtgcggctc catctacaaa ctgggagcca gcggctggaa ggctgccggt
    15421 cccaccaatg tgctgctgtg ccccaggggc tcgcggaccg gctgcagggg caccacgacg
    15481 atcaccctga acgatggttg gggcaggccg taccagaact tcacgatccc caacttcagc
    15541 cagtattatg gaatgaccac gtaccgcgac ttcgacatct atctctggag cgcctccagc
    15601 acggattact ggggcagcca atacgcggtt catggctaca ccacgatcgg gccctacggc
    15661 ctggatggcc tctcgtgggc tatggcgccg cgtccgctgc cgccgacgcc ggtctatccc
    15721 accggaacct cggttccgag ctcgtacacg gtccgctgg
//
LOCUS       DASPJI010000415         2909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_489975, whole genome shotgun sequence.
ACCESSION   DASPJI010000415 DASPJI010000000
VERSION     DASPJI010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489975"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VEL74_11210"
     CDS             <1..220
                     /locus_tag="VEL74_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017315250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG63141.1"
                     /translation="GEDGYSLMRKVRARDATQGGRLPALALTAYARAEDRIQAFAAGF
                     QMHLAKPVDPDELVAVVTSLAGRTGAAS"
     gene            233..>2909
                     /locus_tag="VEL74_11215"
     CDS             233..>2909
                     /locus_tag="VEL74_11215"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HYG63142.1"
                     /translation="MTERDTEQDPSSSLPGGQDRRGSKHPCPIVGMGASAGGLDAFQT
                     FFSRMPPDSGMAFVLVQHLHPRHDTLMPELLARHTSMPVQLVTDRTVAESNRIYVIPP
                     NSTLTIDGCVLYVSRQGGGRGRRAPIDGFFRSLAEDQGDDAVGIILSGAGADGALGLK
                     SIKEHGGMAMVQAPATASYDSMPRSAILTGAADYVLPVEDMPGALMEYLGKLKEARGG
                     DGVEGFREQIEDHLDKIAALLRRKTGHDFSRYKRSTLVRRIRRRMHVLGAPSVSSYLE
                     RLREGPKEADQLFRDLLIGITHFFRDPESFDLLATRVIPRLFEGKGMDEQVRVWVPGC
                     ATGEEAYSIAILLREHLEGLEDPPRVLIFASDIDDQALETARQGWYPESIAGQVSPQR
                     LERFFLRHGNLYQVSREVREMCLFSLHNLITDPPFSRLDLISCRNLLIYLESDLQKKV
                     VPLYHYALRSGGYLFLGPSESVVGHPELFRIVDKRHRIFQRKETVLRSPMSFPLVERG
                     RSGTFRSHFEGARRSNPREPDVSKVLEAILLESYAPACVVVNQRSEVVYLSPRTGRYL
                     ELPAGAPSLHLVEMARDGLRLDLRTALHKAMAGGVPVVHENVPLEPGRDSGSGPRVNI
                     VVRPLPELGDDSGLYMVVFQEVRPAPIEPGAEPGGGAARDAAGTPAGSLAETQQAAVV
                     QRLEAELRSTKDHLQATLEELESSNEELVSSNEELLSINEELQSANEELQTSKEELQS
                     VNEELESVNAELKKKIEQLDRAHSDLQNLFRSTQIATVFVDRDLRIQKFTPAATDVFR
                     LIGSDIGRPIADIAPRFPDGDLLVAIREVLRTLSHHEREVRVAERQAWYILRILPYRT
                     LEDVIDGVVITFADVTELKRAQEQNTQLAAIVESSQ"
BASE COUNT          447 a         1036 c         1007 g          419 t
ORIGIN      
        1 cggcgaggac ggctattcgc tgatgcgcaa ggtgcgggcg cgcgacgcca cccaaggggg
       61 ccgcctgccg gccctggccc tcaccgccta cgcgcgcgcc gaggaccgca tccaggcctt
      121 cgccgccggt ttccagatgc acctcgcgaa gccggtcgat cccgacgagc tggtggcggt
      181 ggtgaccagc ctggccgggc gcaccggggc ggcaagctag tagaatcccc gcgtgaccga
      241 acgggacacc gagcaggacc cctcctcttc actgccgggc ggccaggacc gccgcggcag
      301 caagcatccg tgccccatcg tcgggatggg cgcttcggcc ggcggcctcg acgcgttcca
      361 gacgttcttc tcgcgcatgc cgccggacag cggcatggcg ttcgtgctgg tccagcacct
      421 ccacccccgg cacgacaccc tcatgccgga gctgctggcg cggcacacct ccatgccggt
      481 gcagctggtc accgaccgca ccgtggccga gtcgaaccgg atctacgtca tccctcccaa
      541 ttcgacgctg accatcgacg gttgcgtgct ctacgtcagc cggcaggggg gcggccgggg
      601 ccggcgcgcc cccatcgacg gcttcttccg gtcgctggcc gaggaccagg gggatgacgc
      661 cgtgggcatc atcctctccg gcgccggcgc cgacggcgcc ctggggttga agtccatcaa
      721 ggagcacggc ggcatggcca tggtccaggc gccggcgacg gcgtcctacg acagcatgcc
      781 gcgcagcgcc atcctgaccg gcgcggcgga ctacgtgctg ccggtcgagg acatgccggg
      841 cgccctgatg gagtacctgg ggaagctcaa ggaggcgcgc gggggcgacg gtgtcgaggg
      901 gttccgcgag cagatcgagg accacctgga caagatcgcg gccctcctgc gccgcaagac
      961 cggccacgac ttcagccgct acaagcgcag caccctggtg cgccgcatcc gccgccgcat
     1021 gcacgtgctg ggcgctccat cggtctcctc ctatctggag cggctcaggg agggcccgaa
     1081 ggaggccgac cagctcttcc gcgacctgct catcggcatc acccacttct tccgggaccc
     1141 cgagtcgttc gacctgctgg cgacccgggt catcccccgg ctgttcgagg ggaaggggat
     1201 ggacgagcag gtgcgggtct gggtgccggg ctgcgccacg ggcgaggagg cctactcgat
     1261 cgccatcctg ctgcgcgagc acctggaagg gctcgaggac ccgccccgcg tcctgatctt
     1321 cgcctccgac atcgacgacc aggcgctcga gaccgcccgc caggggtggt atccggagtc
     1381 gatcgccggc caggtctccc cccagcggct cgagcgcttc ttcctccggc acggcaacct
     1441 ctaccaggtg tcgcgagagg tccgggagat gtgcctgttc tcgctgcaca acctgatcac
     1501 cgatccgccg ttctcgcggc tggacctgat ctcgtgccgg aacctgctga tctacctgga
     1561 aagcgacctg cagaagaagg tggtgccgct gtaccactac gccctgcgct ccggcggcta
     1621 cctcttcctg ggcccgtccg agagcgtggt cggtcacccg gagctgttcc ggatcgtcga
     1681 caagcggcac cggatcttcc agcgcaagga gacggtgctg cggtcgccga tgagcttccc
     1741 gctggtggag cgcggccgtt cggggacctt ccggtcccac ttcgaggggg cgcggcggtc
     1801 caatccccgg gagcccgacg tttccaaggt gctcgaggcc atcctgctcg agagctacgc
     1861 gccggcctgt gtcgtggtca accagcgcag cgaggtggtc tacctctcgc cgcgcacggg
     1921 gaggtacctg gagctgccgg ccggcgcccc cagcctgcac ctggtcgaga tggcgcggga
     1981 cggcctgcgc ctcgacttgc ggacggccct ccacaaggcg atggccggcg gcgtgccggt
     2041 ggtccacgag aacgtgccac tcgagcccgg ccgcgactcc ggtagcgggc cccgggtcaa
     2101 catcgtcgtc cggccgctgc cggagctcgg ggacgactcg gggctctaca tggtcgtctt
     2161 ccaggaggtc aggcccgcgc ctatcgagcc gggcgctgag ccgggcgggg gggcggcccg
     2221 ggacgccgcc ggcacgccgg ccgggagcct ggccgagact cagcaggcgg cggtggtcca
     2281 gcggctggag gcggagctgc ggtcgaccaa ggaccacctg caggcgacgc tggaggagct
     2341 ggagagctcc aacgaggagc tggtctcctc gaacgaggag ctgctgtcga tcaacgagga
     2401 gctgcagtcg gccaacgagg agctgcagac gtccaaggag gagctgcagt cggtcaacga
     2461 ggagctggag tcggtcaacg ccgagctcaa gaagaagatc gagcagctcg accgggccca
     2521 cagcgacctc cagaacctgt tccgcagcac ccagatcgcc accgtcttcg tcgaccgcga
     2581 cctgcgcatc cagaagttca ccccggcggc caccgacgtc ttccggctca tcggcagcga
     2641 catcggccgg ccgatcgccg acatcgcccc gcgctttccg gacggcgacc tgctggtggc
     2701 catccgggag gtgctgcgca ccctatcgca ccacgagcgg gaggtgcggg tcgccgagcg
     2761 gcaggcctgg tacatcctgc gcatcctgcc gtaccggacg ctggaggatg tcatcgacgg
     2821 cgtggtgatc acctttgccg acgtcaccga gctcaagcgg gcgcaggagc agaacaccca
     2881 gctcgccgcg atcgtcgaat cctcccagg
//
LOCUS       DASPJI010000416         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_321002, whole genome shotgun sequence.
ACCESSION   DASPJI010000416 DASPJI010000000
VERSION     DASPJI010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321002"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2748)
                     /locus_tag="VEL74_11220"
     CDS             complement(<1..>2748)
                     /locus_tag="VEL74_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63143.1"
                     /translation="FAEIVRVQEALRTRFPEAGGRPVQVVEPPLPLPLPAVDLSGLPS
                     ERRLPEAERLALAESRHPFDLARGPLIRLTLLRLGEGGEESRVLLASLHHIVSDAWSV
                     AIFYRSLTRLYAAFAAGQAARLEEPPVQYADFAVWQRRWLEGEAREREVAYWGRQLAG
                     LPVLELLTDRPRPLTQRFHGAALQLRLPATLSAAVQALARSRYASPFMLLLAGFQALL
                     HRYTGQDDIVVGSPVANRQRGEIEGLIGFFVNTLVLRTGLSGEPSFGELARRVRETAL
                     EAYAHQSLPFEMLVEALEPERDLTRQPLFQVMFQLQNVPLPEVDLGGARLEPLEVESG
                     TAPFDLGVDLIETGEGLALVARYSTDLFDGTTIRRLVEHYGVLLAAALADPERPVGEI
                     PLLGEAETHQLAREWNDTATVVAAGPVHRLFERRAAEVPSAVAVAFADEKLSYGELDR
                     RANLLAHHLIALGVRPGSPVALLLERSVEIAVAMLGVWKAGGAFVPLDPESPRPRLER
                     MLEEVFAGGAPLVVDPDRLALALERDGRPEVPDVLPDVAVGPGSPAYLIFTSGTTGHP
                     KAVRVDHGSLANVLSVSQRDLAWDSRDRMPVLAAYSFDIFLFELWNPLLAGGRADLFA
                     LRPMPDVRRLVVALPAMTRLHAVPSLMRGIVEEIRGQGGRIGGLRTVFVGGEAVPPDL
                     LADLLEVFPGAEVRVLYGPTEGTIICASHEVEDARAWRPLIGRPLGNTLLELRDRRGQ
                     RVPPGVAGEIWIGGAAVSRGYWNRPELTSERFSREDSGWYRTGDLARYRADGVLEYLG
                     RADEQVKVRGFRIEPGEVEAALRDHPAVREAVVVGREDEPGQRRLVAYLTCEPEAAVL
                     DEGLPELESEQVERWRTVYDTDVFGRSAAELEDPAFNVSGWNSSLTGEPIPAEAMREW
                     VG"
BASE COUNT          402 a         1084 c          937 g          325 t
ORIGIN      
        1 cccacccact cccgcatcgc ctcggccggg atcggctccc ccgtgaggct gctgttccag
       61 cccgagacat tgaacgcggg gtcttcgagc tcggcggccg agcggccgaa gacgtcggtg
      121 tcgtagaccg tccgccagcg ctccacctgc tcactctcga gctccggcaa cccctcgtcc
      181 aggaccgccg cctccggctc gcaggtcaga taagccacca gacgccgctg ccccggctcg
      241 tcctcccggc ccacgaccac cgcctcccgc accgccgggt ggtcgcgcag cgccgcctcc
      301 acctcccccg gctcaatgcg gaagccgcgc accttgacct gctcgtcggc gcggcccagg
      361 tactccagga ctccgtcggc ccggtagcgc gccaggtcgc ccgtccggta ccagccgctg
      421 tcctcccggc tgaaccgctc gctggtcagc tccgggcggt tccagtaccc ccggctgacg
      481 gcggcgccgc cgatccagat ctccccggcc acccccggcg gcacccgctg cccgcgccgg
      541 tcccgcagct ccaggagggt gttgcccagc ggccggccga tcagcggccg ccacgcccgc
      601 gcgtcctcca cctcgtggct ggcgcagatg atcgtgcctt cggtggggcc gtagaggacc
      661 cgcacctcgg cccccgggaa cacctccagc agatccgcca gcaggtccgg cggcaccgcc
      721 tcgccgccga cgaagacggt ccgcagaccg ccgatccgcc ccccctgacc gcggatctcc
      781 tcgacgatcc cgcgcatcag cgacggcacc gcgtgcagcc gcgtcatcgc cggcagtgcc
      841 acgaccagac gccgcacgtc cggcatcggc cgcagcgcga acaggtccgc ccggccgccg
      901 gccagcagcg ggttccacag ctcgaacagg aagatgtcga acgagtacgc cgccagcacg
      961 ggcatccggt cccgggagtc ccaggccaga tcgcgctggc tcaccgacag gacgttggcc
     1021 aggctgccgt gatcgacccg caccgccttg gggtgcccgg tcgtgcccga ggtgaagatc
     1081 aggtaggcag gcgagccggg ccccactgcc acatcgggca gcacatcggg cacctcgggc
     1141 cggccatccc gctccagcgc gagggctaga cgatccgggt ccaccacgag gggggcgccg
     1201 ccggcgaaca cctcctcgag catccgctcc aggcgcgggc gcggcgactc cgggtccagc
     1261 ggcacgaagg cgcccccggc cttccagacc ccgagcatcg ccaccgcgat ctccacggag
     1321 cgctccagga gcagagccac cggcgagccc ggccgcaccc ccagcgcgat caggtgatgg
     1381 gccaggagat tggcgcggcg gtcgagctcg ccgtagctca gcttctcgtc cgcgaaggcc
     1441 accgccaccg ctgacggcac ctccgcggcg cggcgctcga acagccggtg caccggcccg
     1501 gcggccacca ccgtcgcggt gtcgttccac tcgcgggcga gctggtgcgt ctcggcctcg
     1561 cccagcagcg ggatctcgcc cacgggccgc tccgggtccg ccagtgccgc ggccagcaac
     1621 acgccatagt gctccaccag acggcggatc gtcgtgccgt cgaacaggtc ggtgctgtag
     1681 cgcgccacca gcgccaggcc ctctccggtc tcgatcaggt ccacaccgag gtcgaacggc
     1741 gccgtcccgg actccacctc caggggctcc agccgcgccc ctcccaggtc cacctccggc
     1801 aggggcacgt tctggagctg gaacatcacc tggaagaggg gctggcgggt caggtcgcgc
     1861 tccggctcga gagcctcgac cagcatctcg aagggcaggc tctgatgggc gtaggcctcg
     1921 agagccgtct cccgcacccg ccgcgccaac tcgccgaagg aaggctcgcc ggacaggccg
     1981 gtccgcagca ccagggtgtt gacgaagaaa ccgatcagcc cctcgatctc tccgcgctgc
     2041 cggttggcca ccggtgagcc caccacgatg tcgtcctgcc ccgtgtaccg gtgcagcagc
     2101 gcctgaaagc cggccagcag cagcatgaat gggctggcgt agcggctccg cgccagggcc
     2161 tggaccgcgg cgctcagcgt tgcgggcagg cggagctgga gggcggcgcc gtggaagcgc
     2221 tgcgtcaggg gacgcggccg gtcggtgagg agctccagga ccggcaggcc ggcgagttgc
     2281 cgtccccagt aggcgacctc ccgttcccgt gcctccccct ccagccaccg ccgctgccag
     2341 accgcgaagt cggcgtactg gaccggcggc tcctccagcc gcgcggcctg tccggcggcg
     2401 aaggcggcgt agagccgggt gagggaccgg tagaagatgg cgaccgacca ggcatccgag
     2461 acgatgtgat ggagcgacgc caggagcacc cggctctcct cgcccccctc tccgaggcgc
     2521 agcagggtca ggcggatgag tgggccccgg gccaggtcga aggggtggcg cgactcggcg
     2581 agggcgagcc gctccgcctc gggcagccgc ctctccgacg gcagcccgga caggtcgacc
     2641 gccggcaggg gaagaggcag cggcggctcc accacctgca cggggcggcc gccggcctcg
     2701 gggaagcggg tgcggagcgc ctcctggacg cggacgatct cggcgaag
//
LOCUS       DASPJI010000417         6190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_834702, whole genome shotgun sequence.
ACCESSION   DASPJI010000417 DASPJI010000000
VERSION     DASPJI010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834702"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>6190
                     /locus_tag="VEL74_11225"
     CDS             <1..>6190
                     /locus_tag="VEL74_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63144.1"
                     /translation="PLRAPLDGEPAFRELVRRVADRLLGARSHAGVPFEKLVEELNPD
                     RGSGHPPLVQVLFGFLSGPPGGAAALPGLGMSLMEVEAGPGAFDLALNLHERAGYVRG
                     WLGYRTDLFERSTAARWSGHLLELLRRAAAEPDRGIGELSLLSAAERHQLLVEWNEAE
                     WETLAGPPVLRRIEEQAERRPDALALVSGGEHVTYGELDRRACLLAAHLRGLGIGLES
                     RVGLRLDRSPAMVAAMLGVWKAGGAFLALPPDLPAERLAWMTKDAGLAAVLEEGPLPE
                     RGSLAGAAALPVLHGSLAYLIYTSGTTGQPKAVMVEHGSLAATLLGSQECFPAGPDDW
                     QPDLFYFSFDASLLDLFGPLRAGATVELLSREEILDLPRLASAFRSASRLHAIPSLLR
                     QVVDFLDTTGEDGGRAARRVFTGGDRVPPELLRDLARAFPAASLTVLYGPTEATIICS
                     SHMASAGEDAERTLIGRPLPGVRLEVVDRWGSPAPIGVAGELCVGGKGVSRGYLGRPD
                     LTAERFTPSPRGSRVYRTGDLVRRWPDGVLEFLGRVDQQVKVRGFRVEPGEVEAALGG
                     LPEVRQAVVVAPEPERRLVAYVVPADAAALAPGAGEQLFTNLRAALARRLPDYMVPSV
                     WVALPALPLSATGKVNRRALPAPAAPRGAGGPETPRRPVEELLAGLWAEVLGLESVGL
                     HGSFFDLGGHSLLATRLLSRVRQALGVELPLAALFDGPTVAELARTAEALLRRKTGGG
                     EELPPVLPRPDPGPAPLSFAQERLWFLDRLEPGPLYNVPVGARLAGPLDVAALAASLG
                     EIVRRHEALRTRFLEERGSPLQEPLPWEPGLVPLSVLDLSELPDRAREAEARRLATQE
                     ARRPFDLASGRLLRLALVRLETRDHLLLLTVHHVAADGWSMGVLLAELSVLYPALSVD
                     LSSPLPEPPVQYVDFAVWQRAWLTGEPLEREVEHWRERLAGAPEVLEIPADRPRHPGG
                     TIQGGQRPFHLAAGVWGGLSALARREGGTPFMGLLAAFQALLSRATGQRDVVVGAPIA
                     NRTRPEIEGLIGFFANTLVLRLDLTGDPSFQELARRARAVSLEAYAHQDVPFEKLVVE
                     LAPERALGRNPLFQAMLVWQDAVPSLSLPGMDVELVEGATGTAKFEMVLALTAAEGGL
                     VGGMEYSADLYDPATIERVLGHLRTLLEGAVADPGTRLSDLPLLTPAERQELAAWSRP
                     PLPYPLPRLVHEMVAARAAAAPEAEAVVFGDEWLSYGELVGRARRMARRLVDLGVGPD
                     VPVGVFLERSPEMMLAILAVLEAGGAYVPLDPSYPEERLRVMLEDSGAPVVVTQAGLA
                     KMVPEGVRVVVDGEGMPGESSPPGPLSHLPPTPPPGEGERRHVTPENLAYVTYTSGST
                     GRPKGVAMTHAAISAMLAWQLRTSAAGGGRTLQFTSLSFDVSFQEIFGTWCAGGAVVL
                     VSEEVRRDPPLLVQLLSEQRVERLFLPFVALQQVAVAAAAGPFPRHLREMMSAGEQLY
                     VTPQVAALFTGLSGAALHNHYGPSETHAVTWLTLEGDPARWPERPTIGGPVDHARTLL
                     LDAGLQPVPRGVPGEIWVGGAGLARGYLGQPALTAERFLPDPFQEIEGWTPGARMYRT
                     GDLARLLPDGELDFLGRADAQVKVRGHRIEPAEVEITLARHPAVRQAAVTVRGESSGA
                     RRLVAYVAFHPEVEAPPFIELRSFLEESLPDPMVPSLWMRLDALPLTPSGKVDRRALP
                     APVTEDDAAGAPHSPVEELLAGIWADVLEVERVGVHDGFFDLGGHSLLATRVMSRVRE
                     AFGIELPLRRLFESPTVSGLARAVEAALAGDAPVAGGISRTPRQGALPLSFSQERLWF
                     VERLGSTGTAYSLPIALHLRGDLDVPALAAVLDEITRRHEILRTVFTDHDGTPAQVVL
                     PFVPRPLPEVDLSGLETAARQGEALRFAGEEAKRPFAVETGPLLRATLAHIAPGERLL
                     LVNMHHIVSDGWSMGVLLRELVALYDAFRAGQPSPLPELPIQYVDFAVWQRQSLSGEV
                     LERHLDYWHRRLTGAPAALELPTDRLRPTVQSFRGGHIDFTLDRHLG"
BASE COUNT          721 a         2198 c         2313 g          958 t
ORIGIN      
        1 gcccctccgc gcgccgctgg acggcgagcc ggccttccgg gagctggtgc ggcgcgtcgc
       61 agaccggctc ctgggggccc ggtcgcacgc cggcgttccc ttcgagaagc tggtggagga
      121 gctgaacccg gaccgcggct cgggtcatcc gccgctggtg caggtgctct tcggcttcct
      181 gagcggcccg ccgggcggag cggccgcgct gccgggcctc ggcatgagcc tgatggaggt
      241 ggaggccggt cccggcgcct tcgacctggc gctcaacctc cacgagcggg ccggctacgt
      301 ccgaggctgg ctgggctacc ggaccgatct cttcgagcgg tccaccgccg cgcgttggtc
      361 cggccatctg ctggagctgc ttcggagagc ggcggcggag ccggaccgtg gcatcggcga
      421 gctgtcgctt ctgtccgccg ccgagaggca tcagctcctc gtcgagtgga atgaggcgga
      481 gtgggagacg ctggccgggc cgccggtgct gcgccgcatc gaggagcagg ccgaacggcg
      541 acccgatgcc ctggcgctgg tcagcggagg cgagcacgtg acctacggcg agttggaccg
      601 gcgggcctgc ctcctggccg cgcacctgcg cgggctcggc atcggcctgg agtcgcgggt
      661 ggggctccgg ctcgatcgtt cgccggcgat ggtggcggcg atgctgggcg tgtggaaggc
      721 cggcggcgcc ttcctggccc tcccgcccga cctccccgcg gagcgtctgg cctggatgac
      781 gaaggacgct ggcctcgccg cggttctcga agaggggccg ctgcccgaac gggggagcct
      841 cgccggagca gcggccctcc cggttctcca cggctccctg gcctatttga tctacacgtc
      901 gggcaccaca ggccagccca aggcggtcat ggtggagcac ggcagcctgg ccgccaccct
      961 tctgggcagc caggagtgtt tccccgccgg tcccgacgac tggcagccgg acctcttcta
     1021 cttctcgttc gacgcctcgc tcctggacct gttcggaccg ctccgggccg gcgccacggt
     1081 ggaactgctc tcccgcgagg agatcctcga cctcccccgg ctggccagcg ccttccggag
     1141 cgccagccgt ctgcacgcca tccccagcct gttgcggcag gtggtggact ttctggacac
     1201 cacgggggag gacggcggcc gggccgcgcg ccgggtcttc accggcggcg accgcgtgcc
     1261 gcccgagctg ctgcgggacc tggcgcgggc cttccccgcc gcttcgctca ccgtgctgta
     1321 cggcccgacc gaggccacca tcatctgctc gtcccacatg gcgagcgccg gggaggacgc
     1381 ggagcgcacc ctgatcggcc ggccgctgcc cggcgtccgc ctcgaggtgg tggaccggtg
     1441 ggggagcccg gcaccgatcg gagtcgccgg cgagttgtgc gtcggcggta agggggtgag
     1501 ccgcggctat ctcggccgcc cggacctcac cgccgagcgc ttcacgccct cgccgagagg
     1561 ctcccgggtc taccggaccg gcgacctggt gcggcggtgg ccggacgggg tgctcgaatt
     1621 cctcggccgc gtcgaccagc aggtcaaggt gcgcggattc cgggtcgagc cgggggaggt
     1681 ggaggcggcc ctcggcgggc tgccggaggt gcggcaggcc gtggtcgtgg cgccggagcc
     1741 cgagcggcgg ctcgtcgcct atgtggtgcc tgccgacgcc gcggccctcg ccccgggggc
     1801 aggggagcag cttttcacaa acctgcgcgc cgccctcgcc cggcgcctgc ccgactacat
     1861 ggtgccgtcg gtctgggtgg cgctgccggc gctgccgctg tcggcgacgg gtaaagtgaa
     1921 ccgccgggcc ctgcccgcgc ccgcggcgcc gcgcggcgcc ggcggcccgg agaccccgcg
     1981 caggcccgtg gaagagctgc tggcgggcct ctgggccgag gtgctgggcc tggagagcgt
     2041 cggcctccac ggcagcttct tcgacctcgg cggccattcg ctcctggcca cccgactatt
     2101 gtcgcgggta cggcaggcgc taggtgtgga actgccgctc gcggcactgt tcgatggtcc
     2161 cacggtggcg gagctggcgc ggacagccga ggcgctcctg cgccggaaga cagggggagg
     2221 cgaggagctg ccgccggtgc tgccgcggcc cgatcctggt ccggcccccc tctccttcgc
     2281 tcaggagcgg ctctggttcc tcgaccggct ggagcccggc ccgctctaca acgtccccgt
     2341 cggcgcgcgc ttggcaggac cgttggacgt ggccgccctg gcggcctccc tgggtgagat
     2401 cgtccgccgc cacgaggctc tgcgcacccg tttcctggaa gagcggggga gcccgcttca
     2461 ggagcctctg ccctgggagc cggggctcgt gccgctgtcc gtgctcgacc tgtcggagct
     2521 cccggaccgg gcccgcgagg ccgaggcccg ccgccttgcg acccaggagg cgcgccggcc
     2581 gttcgatctc gcctcaggac ggctgctgcg cctcgccctg gtgcggctcg agactcggga
     2641 ccacctgctc ctcctgaccg tccatcatgt ggccgcggac ggctggtcca tgggtgtgct
     2701 actcgcggag ctgtccgtcc tctaccctgc gctgtcggtg gacctgtcct cgccattgcc
     2761 ggagccgccc gtccagtacg tcgacttcgc ggtgtggcag cgggcgtggc tgaccggcga
     2821 gccgctggag cgcgaggtgg agcactggcg cgagcgtctc gctggcgcac ccgaggtgct
     2881 ggagatcccc gccgaccggc cacggcatcc gggcggcacg attcaaggcg ggcagaggcc
     2941 gttccatctg gccgccgggg tatggggagg gctgagcgcc ctggcccggc gcgagggggg
     3001 gacgccgttc atggggcttc tcgccgcctt ccaggctctc ctctcccgtg ccaccggcca
     3061 gcgggacgtc gtcgtggggg cgccgatcgc caaccgcaca cggccggaga tcgaagggct
     3121 aatcggcttc ttcgccaaca ctctggtgct gcggctggat ttgacgggcg atccgtcgtt
     3181 ccaggaactg gcgcgacgcg cacgggcggt gagcctcgaa gcctatgccc accaggacgt
     3241 ccccttcgag aagctggtag tggagctggc gccggagcgg gcgctgggcc gcaatccgct
     3301 gttccaggcc atgctggtgt ggcaggacgc ggtgccctct ctctccctgc ccggcatgga
     3361 tgttgagctg gtggaggggg cgaccggcac cgccaagttc gaaatggtcc tcgccctgac
     3421 ggcggccgag ggcggccttg tcggtgggat ggagtacagc gcggacctct acgaccctgc
     3481 caccatagaa cgcgtgctcg ggcatctgcg gactctcctt gaaggcgccg tggccgatcc
     3541 cgggacgcgc ctctccgacc tgccgctgct cacgccggcc gagaggcaag agctggcggc
     3601 ctggagccga ccgccgctgc cgtaccctct gccccggcta gtccacgaga tggtggcggc
     3661 ccgggccgcg gcggcgccag aggccgaggc ggtggtgttc ggcgacgagt ggctgagcta
     3721 tggagagctg gttggacggg cgcgacggat ggctcggcgg ctggtggatc tgggcgtggg
     3781 gccggacgtg ccggtggggg ttttcctgga gcgctcgccg gagatgatgt tggcgatcct
     3841 ggcggtgctg gaggcgggcg gcgcctacgt gccgctcgat ccgagctatc cggaggagcg
     3901 gctgcgggtg atgctggagg actccggggc gccggtggtg gtcacgcagg cggggttggc
     3961 gaagatggtg ccggagggag tgcgggtggt ggtggatggg gaaggcatgc ccggcgagtc
     4021 ctcacccccc ggtcccctct cccatctccc tcccacgcct ccgccgggag agggggagag
     4081 gagacatgtc actccagaga acctagccta cgtgacctat acctcgggct ctaccgggcg
     4141 gccgaagggg gtggcgatga cgcacgcagc catctccgcg atgctcgcgt ggcagctccg
     4201 cacgtcggcg gcgggtggcg ggcggacgct ccagtttacc tcgctctcgt tcgacgtctc
     4261 cttccaggaa atcttcggga cctggtgcgc gggcggcgcg gtggtgctgg tctccgagga
     4321 ggtgcggcgc gatcctcctt tgctggtaca gctcctctcc gagcagcggg tggagcgctt
     4381 gttcctccct ttcgtcgcct tgcagcaggt ggcggtggcc gctgcggccg ggccgttccc
     4441 tcgccacctg cgggagatga tgagcgccgg cgagcagctc tacgtcaccc cacaggtcgc
     4501 cgcgctcttc acgggcctgt cgggagcggc cctgcacaac cactacggcc cgtccgagac
     4561 ccacgccgtc acctggctga cgctggaggg ggatccggcg cgctggcccg agcgccccac
     4621 catcggcggg ccggtggacc acgcccggac gctgctactg gacgcgggac tccagccggt
     4681 cccgcgcggc gtccccgggg agatctgggt cggcggcgcc ggcctagctc gcggatatct
     4741 cggccagccc gccctcacgg ccgagcggtt cctccccgat ccattccagg aaatcgaggg
     4801 ctggaccccg ggggcgcgga tgtaccgcac cggcgacctc gcccggctgc ttccagacgg
     4861 cgagctggac ttcctgggcc gggccgacgc ccaggtgaag gtacggggac accggatcga
     4921 gccggcggag gtggagatca cgctggcgcg tcatcccgcg gtgcggcagg ccgccgtcac
     4981 cgtgcgcggt gagtcgtcgg gcgcgcgccg cctggtggcc tacgtggctt tccatccgga
     5041 ggtggaagcg ccccccttca tcgagctgcg gtccttcctg gaggagagcc tgcccgaccc
     5101 gatggtcccc tccctctgga tgcgcctgga tgcgctcccc ctgactccca gcggcaaggt
     5161 ggaccgccgg gccctgcccg cgcccgtgac ggaagacgac gcggccggcg ctccccactc
     5221 gccggtcgag gagttgctcg ccggcatctg ggccgacgtc ctggaggtcg agcgggtggg
     5281 cgtccacgac ggcttcttcg acctcggcgg gcactcgctg ctggcgaccc gggtgatgtc
     5341 gcgggtgcgg gaggccttcg gcatcgagct gccgctgcgc cgcctgttcg agtcgccgac
     5401 tgtgtccggc ctcgcccggg cggtggaggc ggcgctggcg ggggacgcgc ccgtggccgg
     5461 agggatttcc cggacgccgc gccagggtgc tctgccgctc tcgttctccc aggagaggct
     5521 ctggttcgtc gagcgcctgg gctcgaccgg caccgcctac agcctgccca ttgcactgca
     5581 cctgcgggga gatctcgacg tgcccgccct cgccgccgtg ctggacgaga tcacgcggcg
     5641 ccacgagatc ctgcgcacgg tcttcaccga ccacgacggc accccggccc aggtggttct
     5701 cccgtttgtc ccccggccgc tgccggaggt ggacctgtct gggctggaga cagcggcgcg
     5761 gcagggagag gcgctccgct ttgccggcga ggaggccaag cgccccttcg ccgtggagac
     5821 cggcccccta ctgcgcgcca cgctggccca catcgctccg ggagagcggc tgctgctggt
     5881 caacatgcac cacatcgtct ccgacggctg gtccatgggc gtgctgctgc gcgagctggt
     5941 ggcgctctat gacgccttcc gagcgggcca gccgtccccc ttgccggagt tgccgatcca
     6001 gtacgtggac ttcgccgtct ggcagcggca atcgctctcg ggcgaggtgc tggagcggca
     6061 tctggactac tggcatcggc gccttaccgg cgcgccggcg gcgctggagc tgcccaccga
     6121 ccggctgcgg cccacggtgc agagcttccg cggtggccac atcgacttca ccctggaccg
     6181 ccacctgggc
//
LOCUS       DASPJI010000418         4502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_428362, whole genome shotgun sequence.
ACCESSION   DASPJI010000418 DASPJI010000000
VERSION     DASPJI010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428362"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /locus_tag="VEL74_11230"
     CDS             complement(<1..1700)
                     /locus_tag="VEL74_11230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HYG63145.1"
                     /translation="MIRPALAVLILAGGLLLGGASSAQEAKGALPADLPDRYHTWLEE
                     VAPLIGAGERDAFLALQRDYQRDAFIRKFWAVRDPFPKTSRNELQAAWEERAKAARER
                     FGGLDNDRARMLLFNGEPDNVLRASCNLLAPLEIWSYKGTERINDSFSLVFVNQGGHR
                     LWYPSQGLLMLITMGMAAERVDLSTIQRACPRGDDIAGRLAEALDWKQAEEKVYPRPS
                     DEWLTTFSGQSTDVPAGAAQLPARVDLSFPGRYGSRTVVQTVVSVAREAGATGGMAEG
                     LLVDGEVLRNDELFENFRYRFAPPANAASGAQPAETLPLVFQRYLRPGRYTFIVKVED
                     PAGQRFFREQRELEVPSVEAAAAAAAPAVSTAPAVGAPSAPAAVPALAEANAGDLGSD
                     ETSIRLLPPKEGLLTGKVRLEAITTGEGIARVSFELDGKPVLTKGKPPYSVELNLGAQ
                     PRLQRITARALAADGRQLAEDELQLNSGPHRFSIRLVEPQDGKVYRESLRAHAQVEVP
                     EGETLERVELYLNDGLVATLYQPPFIQPILLPGAAENSYVRAVAYMKDGNSTEDLVLI
                     "
     gene            complement(1697..4396)
                     /locus_tag="VEL74_11235"
     CDS             complement(1697..4396)
                     /locus_tag="VEL74_11235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG63146.1"
                     /translation="MSDPKRLHSRPLGRTPARRLLLLILAAALLPSASAWPAEQPAEQ
                     PAAPPAAAPQQAPPDGAAAPGSGEKADDKKAERAKKMTRKEIKEATEKLPGKYKTWLE
                     EVDVLITDDERQSFLELEKDYQRDAFIKRFWEVRDTFKGSRNEFYDRYQVRLAEARAA
                     FDNNLKDQRARIFLLNGPPTVLLRPRCSVLVPVEAWYYRESERLNFEFIVVFYRRWGA
                     GPFMIWQPAEGLNALFQDANAANAGPSRTRNLNAVVEGCIDGDKVAAAINWVARQGIG
                     YSSIQARFEGTPEGPGGEWIATFDSYSTDLPPDAPLLPASLEVGFPGRHQNRTVLQGV
                     VDVPASAAGQIQLADHRSHNFVLTGEILQGKELFDSFRYKFDFPVGDQTADGGLPLIF
                     QRYLRPGDYKMILKLEDLNAKKFFRIEKPLKVPQVDTAMPPPPPTTPQEIESARMLAE
                     ANAAISNGETTVKIIQPHGELQTGMLRFDTMTTGTNIDKVTFALDGKPLLTKRKPPFS
                     VELDMGSVPRTRTLSATAYDAGGNQLAGDELLVNAPGHRFRVKLVEPQRGKRYQSSLL
                     ARAETEVPEGEAVDRVEFYLNETLIATLYQEPYVQPIVLPKEEPVAYVRTVAYLTDGN
                     STEELVFVNAPDNLEELEVQYVELYTSVLDRGGRPVEGLEQKDFTVAEDGTRQEVSRF
                     EKVTDLPIHATVALDVSASMDDNVQTAREAALQFFQQAIRPKDRGALITFNDRPTLAV
                     KFTKDPNTLAGGLAGIKAERGTALYDTVIFSLYYFNGIKGQRALLLLSDGKDESSRFS
                     FEDAVDYARRAGVTIYTIGLGEDLDRKKLTRLAEETGGRSFFLQDVNDLAGIYTEVEK
                     ELRSKYLIAYQSSNTSNESTFRSVEVKVNRPGMEAKTIRGYYP"
BASE COUNT          597 a         1466 c         1683 g          756 t
ORIGIN      
        1 ttgatcagca ccaggtcctc ggtggagttg ccgtccttca tgtaggcgac ggcgcggacg
       61 tagctgttct ccgcggcgcc cgggagcagg atgggctgga tgaacggcgg ctggtagagc
      121 gtcgccacca ggccgtcgtt caggtagagc tcgacgcgct ccagggtctc cccctcgggg
      181 acctcgacct gggcgtgggc gcgcaggctc tcgcggtaga ccttgccgtc ctgcggctcc
      241 accagccgga tggagaagcg gtgcggcccg gagttgagct gtagctcgtc ctcggcgagc
      301 tggcggccgt cggcggccag ggcgcgggcg gtgatgcgct gcagccgcgg ctgggcgccg
      361 aggttcagct cgacgctgta cggcggcttg cctttggtga gcaccggctt gccgtcgagc
      421 tcgaagctga cgcgggcgat cccctcgccg gtggttatgg cctccagccg caccttgccc
      481 gtgagcagcc cctcctttgg ggggaggagg cggatggagg tctcgtccga gccgaggtcg
      541 ccggcgttgg cctcggccag cgccggtacg gcggctggag cgctcggagc gcccaccgcg
      601 ggggccgtgg acacggccgg agcggcggcc gccgcggccg cctccaccga cggcacctcc
      661 agctcgcgct gctcccggaa gaagcgctgg cccgccgggt cctccacctt gacgatgaac
      721 gtgtagcggc ccgggcgcag gtagcgctgg aagacgaggg gcagggtctc cgccggctga
      781 gcgcctgacg ccgcgttggc gggcggggcg aagcggtacc ggaaattctc gaacagctcg
      841 tcgttgcgga gcacctcgcc gtcgaccagc aggccctccg ccatgccgcc ggtagcgccg
      901 gcctcgcgcg ccacgctcac cacggtctgg accaccgtgc ggctgccgta gcggccgggg
      961 aacgacaggt cgacccgggc cgggagctgc gccgcgccgg ccggcacgtc ggtcgactgg
     1021 ccgctgaagg tggtgagcca ctcgtcgctg ggcctggggt agaccttctc ctcggcctgc
     1081 ttccagtcga gggcctcggc gaggcggccg gcgatgtcgt cgccgcgcgg gcaggccctc
     1141 tggatggtgg agaggtcgac ccgctccgcc gccatgccca tggtgatcag catcaggagg
     1201 ccctggctcg ggtaccagag ccggtggccc ccctggttga cgaagaccag ggagaaggag
     1261 tcgttgatgc gctccgtgcc cttgtagctc cagatctcca gcggcgcgag gaggttgcag
     1321 ctcgcgcgca ggacgttgtc gggctcgccg ttgaagagca gcatgcgggc gcggtcgttg
     1381 tcgaggccgc cgaagcgctc gcgggcggcc ttggcccgct cctcccaggc cgcctgcagc
     1441 tcgttcctgc tcgtcttggg gaaggggtcg cggaccgccc agaacttgcg gatgaaggcg
     1501 tcgcgctggt agtcgcgctg gagggccagg aaggcgtcgc gctccccggc cccgatcagc
     1561 ggcgccacct cctccagcca ggtgtggtag cggtcgggga ggtcggccgg cagggcgccc
     1621 ttcgcctcct gggcggagga cgccccgccc aggagcaggc cgccggcgag gatcaggacg
     1681 gccagcgccg gccggatcac gggtaatagc cccggatcgt cttggcctcc ataccgggcc
     1741 ggttcacctt gacctcgacg gaacggaagg tgctctcgtt gctggtgttg gacgactggt
     1801 aggcgatgag gtacttggag cgcagctcct tttcgacctc cgtgtagatg cccgccagat
     1861 cgttgacgtc ctggaggaag aagctgcggc cgccggtctc ctcggccagg cgggtgagct
     1921 tcttgcggtc gaggtcctcg cccaggccga tggtgtagat ggtgacgccg gcacgccgcg
     1981 catagtcgac ggcgtcctcg aacgagaagc ggctgctctc gtccttgccg tcggagagca
     2041 gcagcagggc gcgctgcccc ttgatgccgt tgaagtagta gagcgagaag atcaccgtgt
     2101 cgtagagcgc cgtgccgcgc tcggccttga ttcccgcgag gccgccggcg agggtgttcg
     2161 ggtccttggt gaacttcacc gccagcgtcg gccggtcgtt gaaggtgatc agggcgccgc
     2221 ggtccttggg acggatcgcc tgctggaaga actggagcgc cgcctcgcgg gcggtctgca
     2281 cgttgtcgtc catggaggcc gagacgtcga gggcgacggt ggcgtggatc ggcaggtcgg
     2341 tgaccttctc gaaccggctc acctcctgcc gggtgccgtc ctcggcgacg gtgaagtcct
     2401 tctgctcgag cccctcgacc ggccggccgc cgcggtcgag caccgaggtg tacagctcga
     2461 cgtactggac ctccagctcc tcgaggttgt cgggggcgtt gacgaagacc agctcctcgg
     2521 tcgagttgcc gtcggtcagg taggcgacgg tgcggacgta ggcgaccggc tcctccttgg
     2581 gcagcacgat cggctgcacg tacggctcct ggtagagcgt cgcgatgagc gtctcgttga
     2641 ggtagaactc cacccggtcg acggcctcgc cctcgggcac ctcggtctcg gcgcgggcga
     2701 ggaggctgct ctggtagcgc ttgccccgtt gcggctcgac cagcttcacc cggaaacggt
     2761 ggccgggggc gttgaccagc agctcgtcgc cggcgagctg gttgccgccg gcgtcgtagg
     2821 cggtggccga gagcgtccgc gtgcgcggca ccgagcccat gtccagctcg acgctgaacg
     2881 gcggcttcct cttggtgagg agcggcttgc cgtcgagcgc gaaggtcacc ttgtcgatgt
     2941 tggtgccggt ggtcatggtg tcgaagcgca gcatgccggt ctgcagctcg ccgtgcggct
     3001 ggatgatctt gaccgtcgtc tcgccgttcg agatggcggc gttggcctcg gcgagcatgc
     3061 gggcgctctc gatctcctgc ggcgtggtgg gcggcggcgg gggcatcgcc gtgtccacct
     3121 ggggcacctt gagcggcttc tcgatgcgga agaacttctt ggcgttcagg tcctcgagct
     3181 tgaggatcat cttgtagtcg ccgggccgga ggtagcgctg gaagatcagg gggaggccgc
     3241 cgtcggccgt ctggtcaccc acggggaagt cgaacttgta gcggaagctg tcgaacagct
     3301 ccttgccctg caggatctcg ccggtgagga cgaagttgtg ggagcggtgg tcggcgagct
     3361 ggatctggcc ggcggcgctg gccggcacgt cgaccacccc ctgaagcacc gtccggttct
     3421 ggtgacggcc ggggaagccg acctccagcg acgccggcag cagcggcgcg tcgggcggca
     3481 ggtcggtcga gtaggagtcg aacgtggcga tccactcgcc gcccggccct tcgggcgtcc
     3541 cctcgaagcg cgcctgaatg gaggagtagc cgatcccctg ccgcgccacc cagttgatgg
     3601 cggcggcgac cttgtcgccg tcgatacacc cctcgacgac cgcgttgagg ttgcgggtgc
     3661 ggctcgggcc ggcgttggcg gcgttggcgt cctggaagag ggcgttcagc ccctcggcgg
     3721 gctgccagat catgaacggc ccggcgcccc agcggcggta gaagacgacg atgaactcga
     3781 agttcaggcg ttcgctctcc cggtagtacc aggcctccac gggtaccagc acgctgcagc
     3841 gcggccggag cagcaccgtc ggcgggccgt tcagcaggaa gatccgggcc cgctggtcct
     3901 tgaggttgtt gtcgaaggcg gcgcgcgcct cggccaggcg cacctggtag cggtcgtaga
     3961 actcgttgcg cgaccccttg aaggtgtcgc gcacctccca gaagcgcttg atgaacgcgt
     4021 cccgctggta gtccttctcc agctccagga agctctgccg ctcgtcgtcg gtgatcagga
     4081 cgtccacctc ttcgagccag gtcttgtact tgcccggcag cttctcggtg gcctccttga
     4141 tctccttgcg ggtcatcttc ttggccctct cggccttctt gtcgtccgct ttctcgccgg
     4201 acccgggggc ggcggcgccg tccggaggcg cctgctgggg cgcggcggcg ggcggggccg
     4261 cgggctgctc ggcgggctgc tcggcgggcc aggcggaggc ggacgggagg agggcggcgg
     4321 cgaggatcag caggaggaga cggcgggcgg gcgtcctgcc aagaggacgc gagtggagcc
     4381 ttttggggtc ggacatcatg taccctttga cgacctcgat cgagagtcta accacttcaa
     4441 tacgcgggcg ggccggcgag ccattccggc gggacctcac cttgccccca cggttacaat
     4501 ag
//
LOCUS       DASPJI010000419        10172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_659436, whole genome shotgun sequence.
ACCESSION   DASPJI010000419 DASPJI010000000
VERSION     DASPJI010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10172)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10172
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659436"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1789
                     /locus_tag="VEL74_11240"
     CDS             <1..1789
                     /locus_tag="VEL74_11240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG63147.1"
                     /translation="AAKTELPQAAAPLEIGQVLIDFPDLQSQRMITRTLVTIPAASLK
                     AAPGEKGDEVRVVVEGLVEQASGVFEDFRVRFKVPAPKAAEPVALSFERQLRPDRQFL
                     MRLRVRDEVSGAEAMLTRGFKVPRLPEPSPEWDAINVAQGEEMKVALQTADSLVLLPP
                     AEEVVFGLWRADALVTGERIQKVTFLLDGKAQVSRGRPPFTAEIRLPAVPTEGIVRAE
                     GYDAQNRLVAADEVVLNRPREAFRVAVTEPAEGAAVSGRTRVTAEVTVPEARKVEKVE
                     FSLNDQPVATLTQPPWQVQVDIPGDEIVYVTVAATLDNDDRLEEVRFLRAPENLEHID
                     VQLVELFATVTDRSGQVVRGLVADDFEVLEQGKPQPLSKFELVENLPLTVGLVLDTSG
                     SMEASLSEAQRAAADFLKSVVKPKDRAFAMQFAGKPNLLIAPTDDIEAVSQSLQNFQA
                     VGMTALHDAVVASLHYFRSTRGQRALVLLSDGDDTASAVSFEDALEYARRSGVAVYTI
                     GLKVGLGGVSVRNKLNRLAESTGGRSFYIDKAEELSSVYGQIEQELRSRYLLAFQTQH
                     PEGEKTYRAVEVRVKKSGLKARTARGYYP"
     gene            1786..4407
                     /locus_tag="VEL74_11245"
     CDS             1786..4407
                     /locus_tag="VEL74_11245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG63148.1"
                     /translation="MRALALAAVLLAGVSGAAAAAAQDTDPVLWPEEQRAFFQDGPGL
                     LLSDEQRSAFLAMDEAARGAFVRELLGRDPVPGTPDNELLLGIERRRRLATQTFPSPL
                     DVRAQLLFLRGEPAERRIIDCAAGFRPLEIWVYGTPPEPLRPLVVYKPDTSEPFRIWL
                     PIDAKRALYNPGMIGLMEDAARMSRGRGAPRRLDAQICPDARLVDRATGIAGLGGPES
                     GLPEEDGEVVARITLDSTLDDLPEGRKDPGKPVAFRWVAPDDRARVLERPKDLAAWAR
                     EASLTKLPEEPRELELGSVELDFPRRQGQRMAVRTLIPVPGGALQVTTEKSKPAVHLT
                     VTAVVEQGGQVFEDLRVRYRLPEPKQGEVVALLLERQLRPDLPFLLRLKVRDDVSGAE
                     TRIARGFLVPSQPVVRLGAAARTAALGQIIVPEGGVGKNSIVLPPPPDQVVLNVWRAD
                     PLVNGERIVKVVFLVDGQSQLSRTRPPYSAEVRLASFPKEQTVRVEGYDENGELVAAD
                     EVVINRARGTFRVTLTEPKAGSKLAGKVRARAEVVVPEERRVESVDLKVNDVVVATLT
                     APPWEAQIEVPAREEIVYVSAVAKLDDGSQAEDTQFLRAPANLEQVDVHLVELYTAVT
                     DGAGNLVQDLQAADFEVTEGGTAQALSKFELVKNLPLNVGLVLDTSTSMASSLAEAQR
                     AASEFLTKVVTPRDRCFALGFGSRPYLLMPPTDDAEAVAQAVEGVRAVGATALHDALV
                     TALYYFRAGQGQRALVLLSDGDDTASHTTYERALEFAQRSGVTVYSVGLGLSALDFEA
                     RGKLTQLAETSGGRAFFISRAEELEAVYGRIEEELRSRYLLAYQTERAEEGGFRPVDV
                     KVKKRGLKARTARGYYP"
     gene            4503..7127
                     /locus_tag="VEL74_11250"
     CDS             4503..7127
                     /locus_tag="VEL74_11250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG63149.1"
                     /translation="MRALLGLLLAALPVIAAEPVPPPPPAVDPLALPEEQRAFFQDGP
                     GLLLTEKQRVEFLWNTDAGRRRLIDKFWERDPVPETPENELRLGIEKRRRLALLHFPS
                     PLDVRWQILFLRGLPTERRQVTCRALNPLEIWEYGPGQPPLVIYQPLPRTLPSPTSRL
                     WWPEDTLIVLYDAYLLPNSLPKKGEALAAELQKLGRSARATCPAFTEVERATGAAAGS
                     DSREARKTQDFELPEDRVWSVLRPADLAAWSRAAAATAVTLPPPLATGAVELDFPRTL
                     GEQLTVRALLPVSSTGSGARIIVSALTEGEGKVLDEYRAVYRLPEGGRGGEAVPLLLE
                     RQVPPGLPFLLRLRVRDEATGAEALVTRSAVAPSMPEARDPEVVRAAVLGMPLLLGDW
                     VGPDSLLLPPPPERAAPGIWRVEPMITGRRITRVVYRVDGQVWTTLDRSPFAADVLLT
                     GEAREQVIRAEGYDEDNLLVAVDEVAVPGEQREQEGSGARPFRADLETPAPVRAGGGT
                     VLARASVSVPEGRRVETVEIRLNDAPVTTLTAPPWEARVDVPASEPMVYLSAVARLDD
                     GSQAEDVHFLRAPDNLQEIDVRLVELYATVTDRSDRPVADLRERDFEILEEGRPQALA
                     RFERVQDLSLNLGLVIDSSHSMTESIAETQRAAAGFLSRLIRPQDRSFVIGLAARPYL
                     LFAPTNDAAAMAKASLPELRARGGTALYDSLATAVLLLREGRGQRAIVLLSDGGDSLS
                     HATLQQAVDLARQGGVTIYAIGLGVRTPAAPLVAVGAPLSHEQQARQLERMTERRPRA
                     RHTPLDDLAQETGGRVFYIDKAKELEEVYGQIEEDLRSRYLLAYQTERGEDPGDPSVE
                     VRVKKRGLKARTARGYYP"
     gene            7177..9246
                     /locus_tag="VEL74_11255"
     CDS             7177..9246
                     /locus_tag="VEL74_11255"
                     /inference="COORDINATES: protein motif:HMM:NF015530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HYG63150.1"
                     /translation="MTRYGIIGVCSLAAALGTGALAPAAAQPPAPATGKPAGKPASNP
                     IVERIGNRAFIQVESPSFANLTPKQKLLAYHLSRAAIQLDPVFYDQMSDYGIPAKRLL
                     GALVERPERLPAAMRQPVVDYAKQFFANSGNHDGATGRKFLPAFSFEDFAEAAEQARQ
                     KGAPLGTKDQLKTVLGELRQPLFDPNYQVSVTNKNPPPGEDILTASSNNLYQGVTLED
                     LKGFTERFPLNSRLVKKDGKIVEEVYRAGTPDGKVPPGLYARELKAVIRELEQAIPYA
                     EPDQAKVLQALIRFYQTGSAEDWAAYNVLWIRNDPEVDMASGFIEVYRDARGAKGSAQ
                     TVVSVRDKELGPLMQKLAANAVYFEGKAPWADKYKKLDVEPPVGKAIETVIATGDFTV
                     NTVGVNLPNEQDIREKHGTKSVLLTNASRALNATRGVKLATEFSMEPDEQELFEKHGT
                     MAGVLHVAMHEVIGHGSGKVAVPNDPRTYLREYYSTLEEARADLVSYWNIYDPKLAEL
                     GVEDVQGVAKELYRALARQGLTTLNRYPTGDEAEEDHDRNRLLIVNYLIEAGAFERLQ
                     KDGHWYITVKDYDKAHATVGKLLAELMRIKAEGDYDAIKALVDKYGVHFDPAVRDDVV
                     ARFKKLDIPTYYTGIYPDFAPKKDKSGKVTAVEVSYPRDFLRQQLEWARLNGTLGFTA
                     DGKQATK"
     gene            9243..9908
                     /locus_tag="VEL74_11260"
     CDS             9243..9908
                     /locus_tag="VEL74_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63151.1"
                     /translation="MTRAVPSAALLLATLLVAGGALSQAEAPGQLQFARLNRTYSDVI
                     TEIEPIRQGPVTVRLSSPRHQLTVRNHLLRLEPGTGGSHSAELRVDFSGKGWLVADVD
                     VAGMGGRLEDDVTVPAQSQTVEGRARVRKVAEGYLITPEQLPKSVRVRIQSGVGSRIA
                     GFCDRAAALLFSNVDCGGLNRALSTAVVPLPAAGESYLLENAELTAAERQRIDSYLAR
                     ESR"
     gene            9911..>10172
                     /locus_tag="VEL74_11265"
     CDS             9911..>10172
                     /locus_tag="VEL74_11265"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYG63152.1"
                     /translation="MRASRGTPVTLLLLVAIGAVFVLETLRGGSTNPEVLIALGANHP
                     ELVLNQGEWWRLVTSMFLHIGIAHLLLNGWALFQIGAIVESWM"
BASE COUNT         1527 a         3704 c         3544 g         1397 t
ORIGIN      
        1 ggcggcgaag accgagctcc cccaggccgc ggcgccgctg gagatcggtc aggtcctgat
       61 cgactttccg gacctccaga gccagcgcat gatcacccgc accctggtca ccatcccggc
      121 ggcgagcctc aaggccgctc cgggagagaa gggggacgag gtccgcgtcg tggtggaggg
      181 gctcgtcgaa caggcgagcg gcgtgttcga ggacttccgg gtgcgcttca aggtgccggc
      241 ccccaaggcg gccgagccgg tcgccctctc cttcgagcgc cagctccgcc ccgaccggca
      301 gttcctgatg cgcctgcggg tccgggacga ggtgagcggc gccgaggcga tgctcacccg
      361 cggcttcaag gtgccacgcc tgcccgagcc gtcgcccgag tgggacgcga tcaacgtcgc
      421 ccagggcgag gagatgaagg tggcgctcca gaccgccgac agcctcgtcc tcctccctcc
      481 ggccgaggag gtggtgttcg gcctctggcg cgccgacgcc ctggtaacgg gggagcgcat
      541 tcagaaggtg accttcctgc tcgacggcaa ggcccaggtc tcccgcggca ggccgccgtt
      601 caccgccgag atccgcctcc ccgccgtccc taccgagggg atcgtgcggg ccgaggggta
      661 cgacgcccag aaccggctgg tcgccgccga cgaggtggtg ctcaaccgcc cgcgcgaggc
      721 cttccgggtg gcggtcaccg agcccgccga gggggccgcg gtctccggcc gcactcgcgt
      781 caccgccgag gtgaccgtcc ccgaggcgcg gaaggtggag aaggtggagt tcagcctcaa
      841 cgatcagccg gtggccaccc tcacccagcc cccctggcag gtccaggtgg acatccccgg
      901 cgacgagatc gtctacgtca ccgtcgccgc caccctggac aacgacgacc gcctcgagga
      961 ggtgcgcttc ctgcgcgccc ccgagaacct ggagcacatc gacgtccagc tcgtcgagct
     1021 gttcgccacc gtcaccgacc gctccggcca ggtggtgcgc ggcctcgtcg ccgacgactt
     1081 cgaggtgctg gagcagggca agccgcagcc gctctccaag ttcgagctgg tggagaacct
     1141 gccgctgacc gtcggcctcg tcctcgacac ctcgggctcc atggaggcgt cgctctccga
     1201 ggcccagcgc gccgcggcgg acttcctgaa gagcgtcgtc aagccgaagg accgggcctt
     1261 tgccatgcag ttcgccggca agcccaacct cctgatcgcg ccgacggacg acatcgaggc
     1321 cgtctcccag tcgctccaga acttccaggc ggtgggcatg accgccctcc atgacgccgt
     1381 cgtggcgagc ctccattact tcaggtcgac ccgcgggcag cgcgccctgg tcctgctctc
     1441 cgacggcgac gacacggcga gcgccgtctc cttcgaggac gccctggagt acgcccgccg
     1501 tagcggcgtc gccgtctata ccatcggact caaggtgggg ctgggcggcg tatcggtccg
     1561 caacaagctg aaccggctgg cggagagcac cggtggccgc agcttctaca tcgacaaggc
     1621 cgaggagctg tccagcgtct acgggcagat cgagcaggag ctgcgcagcc gctacctgct
     1681 ggctttccag acccagcacc cggaggggga gaagacctac cgggcggtcg aggtacgggt
     1741 gaagaagtcc ggtctcaagg cgcgcacggc ccggggttac tacccgtgag ggcccttgcc
     1801 ctcgccgccg tcctgctcgc cggggtctcc ggggccgccg cggcggctgc tcaggacacc
     1861 gatcccgtcc tctggcccga ggagcagcgg gcgttcttcc aggacggccc cggcctcctg
     1921 ctgtccgacg agcagcggtc cgccttcctg gcgatggacg aggcggcgcg cggggcgttc
     1981 gtgcgcgagc tcctcgggcg tgacccggtc ccgggaacgc cggacaacga gctgctcctg
     2041 gggatcgagc gccgccggcg cctcgccacc cagacgttcc cgtcgccgct ggacgtccgc
     2101 gcccagctcc tgttcctgcg cggcgagcca gcggagcggc ggatcatcga ctgcgccgcc
     2161 ggcttccggc cgctggagat ctgggtctac ggcacccctc ccgagccgct ccggccgctg
     2221 gtggtctaca agcccgacac cagcgagccg ttccgcatct ggctgcccat cgacgccaag
     2281 cgcgccctct acaacccggg gatgatcggc ctcatggaag acgccgcgcg gatgtcccgc
     2341 ggccgcggcg cgccccggcg cctcgacgcc cagatctgcc cggacgcccg gctggtggac
     2401 cgcgccaccg gcatcgccgg cctcggcggc ccggagagcg ggctgcccga ggaggacggc
     2461 gaggtcgtgg cgcgcattac cttggacagc accctcgacg atctgccgga gggcaggaag
     2521 gaccccggca agcccgtggc gttccggtgg gtggcgccgg acgaccgggc gcgcgtcctg
     2581 gagcggccga aggacctggc ggcctgggcc cgcgaggcgt ccctcaccaa gctgcccgag
     2641 gagccacgcg agctggagct ggggtcggtc gagctggact tcccgcgccg gcaggggcag
     2701 cggatggcgg tccgcaccct gatcccggtg cccggcggcg ccctgcaggt gaccaccgag
     2761 aagagcaagc ccgcggtgca cctcaccgtc accgcggtgg tggagcaggg gggccaggtg
     2821 ttcgaggacc tccgcgtgcg ctatcgcctg cccgagccca agcagggcga ggtggtggcc
     2881 ctgctcctcg agcgccagct ccgtccggac ctgcctttcc tcctgcgcct caaggtgcgg
     2941 gacgacgtga gcggcgccga gactcggatc gcccgcggct tcctggtgcc gtcccaaccg
     3001 gtggtgcggc tcggcgccgc cgcccgtacc gcggctctgg ggcagatcat cgtccccgag
     3061 ggcggggtgg ggaagaacag catcgtgctg ccgccgcctc cggaccaggt ggtgctcaac
     3121 gtctggcgcg ccgacccgct ggtcaacggc gagcgcatcg tcaaggtggt cttcctggtg
     3181 gacggccagt cgcagctctc ccgcacccgg ccgccgtact ccgccgaggt gcgcttggcg
     3241 tcgttcccca aggagcagac cgtccgcgtc gagggctacg acgagaacgg cgagctggtg
     3301 gcggccgacg aggtggtgat caaccgcgcc cgcggcacct tccgggtgac cctcaccgag
     3361 ccgaaggccg gctccaagct cgcgggcaag gtacgcgccc gcgccgaggt ggtggtgccc
     3421 gaggagcggc gggtcgagtc ggtggacctc aaggtcaacg acgtggtcgt ggcgaccctc
     3481 accgcgcccc cctgggaggc gcagatcgag gtgccggcgc gcgaggagat cgtctacgtc
     3541 tcggcggtgg cgaagctcga cgacggcagc caggccgagg acacgcagtt cctgcgcgcg
     3601 cccgccaacc tggagcaggt ggacgtccat ctcgtcgagc tctacaccgc ggtgaccgac
     3661 ggcgccggca acctggtgca ggacctccag gccgccgact tcgaggtgac cgaggggggc
     3721 acggcccagg cgctcagcaa gttcgagctg gtcaagaacc tgccgctgaa cgtcggcctg
     3781 gtcctcgaca cctcgacctc catggcgtcg tcgctcgccg aggcgcagcg cgcggcctcg
     3841 gagttcctca ccaaggtggt gacgccgcgc gaccgctgct tcgccctcgg cttcggcagc
     3901 cgtccctatc tgctgatgcc gccgacggat gacgccgaag ccgtggccca ggcggtggag
     3961 ggggtgcggg cggtgggggc cacggcgctc cacgacgccc tggtgaccgc cctctattac
     4021 ttccgggccg gccaggggca gcgcgccctg gtgctgctct cggacggcga cgacacggcg
     4081 agccacacca cctatgagcg ggcgctggag ttcgcccagc gcagcggcgt caccgtctat
     4141 tccgtcggcc tgggcctgtc ggccctcgac ttcgaggcgc ggggcaagct gacccagctc
     4201 gccgagacca gcggcggccg cgccttcttc atcagccgtg ccgaggagct ggaagcggtc
     4261 tatggccgga tcgaggagga gctgcgcagc cgctacctcc tcgcctacca gaccgagcgc
     4321 gccgaggaag gcggcttccg gcccgtcgac gtcaaggtca agaaacgggg cctcaaggcc
     4381 cgcaccgcga gaggttacta cccgtgagcc cagacccccg gcctcttccc cgccacgccg
     4441 ctcccacgag tctccccctc tcccggggag gggggcgggg aggcgggaga gggggccggg
     4501 gggtgagggc cctcctcggc ctcctcctcg ccgctctccc agtcatcgcc gcggagccgg
     4561 tgcctccccc tccgcccgcc gtcgatccgc tggcccttcc cgaggagcag cgagcgtttt
     4621 tccaggacgg tcccggcctc cttctcaccg agaagcagcg cgtcgaattc ctatggaaca
     4681 cagacgccgg ccgccggagg ctcatcgaca aattctggga gcgtgacccg gtcccggaga
     4741 cgccggagaa cgagctgcgg ctcggcatcg agaagcgccg gcgcctggcg ctcctgcact
     4801 tcccgtcccc cctggacgtc cgctggcaga tcctgttcct gcgcggcctg cccacggaac
     4861 gccgacaggt cacctgccgg gccctgaatc cgctggagat ctgggagtat gggccgggcc
     4921 agccgccgct cgtcatctat cagccgctcc ctcgcaccct cccgtctccc acgtcccgtc
     4981 tctggtggcc cgaggatacc ctgatcgttc tctacgacgc ctaccttctc ccgaacagcc
     5041 tcccgaaaaa gggggaggcg ttggcggcgg agctacaaaa gctggggcgg agcgcgcggg
     5101 cgacctgtcc cgccttcacg gaggtggagc gtgccaccgg cgctgccgcg ggtagcgaca
     5161 gcagggaggc ccggaagacg caggatttcg agcttccgga ggaccgggtc tggtcggtgc
     5221 tgaggccggc cgacctggct gcttggtcgc gcgcggccgc ggccacggcc gtgaccttgc
     5281 cgccgccgct ggcgacgggc gccgtggagc tggacttccc acgcaccctg ggagagcaac
     5341 tgacggtccg cgcgctgttg ccggtctcgt caaccggctc gggggcgcgg atcatcgtgt
     5401 ccgccttgac cgaaggcgag ggcaaagtcc tcgacgagta ccgtgccgtt taccggctgc
     5461 cggaaggggg ccgcggcggt gaggccgtgc cgctgcttct ggaacgccag gtccctccgg
     5521 ggctcccctt cctcctgcgt ctccgggtgc gcgacgaggc gaccggcgct gaggccctgg
     5581 tgacgcggag cgccgtagcg ccgagcatgc ccgaggcgcg agaccccgag gtcgtccgcg
     5641 ccgccgtact gggcatgccg ctcctcctgg gggattgggt ggggcctgac agcctgctgc
     5701 tgcctccgcc gcccgaacgc gccgccccgg gtatctggcg cgtcgagccg atgatcaccg
     5761 gccgccgcat cacccgcgtt gtctaccggg tggacggcca agtctggact actctcgacc
     5821 ggtctccctt cgccgccgat gtgctcctta ccggagaggc ccgggagcag gtgatccgcg
     5881 ccgagggcta cgacgaggac aacctgctgg tagcagtgga cgaggtggcg gtcccggggg
     5941 agcagcggga gcaggagggc tccggagcgc gccccttcag ggctgatctg gagactccgg
     6001 cccctgtgcg agccggcggc ggtacggtcc tcgcccgggc cagcgtcagc gtccccgagg
     6061 ggcggcgggt cgagacggtg gagatccgcc tcaacgacgc gccggtcacg accctgacgg
     6121 cgcccccctg ggaggcgcgg gtcgacgtcc cggccagcga gcccatggtc tacctctccg
     6181 ccgtcgcccg cctggatgac ggcagccagg ccgaggacgt ccacttcctg cgcgcgccgg
     6241 acaacctgca ggagatcgac gtccgtctgg tggagctgta cgccacggtg accgatcgct
     6301 cagaccggcc ggtggcggac ctcagggaaa gagacttcga gatcctggag gagggccggc
     6361 cgcaggctct cgcccggttt gagcgggtcc aggacctctc cctgaacctc gggctcgtca
     6421 tcgacagctc gcactcaatg accgagtcca tcgccgagac ccagcgggcg gccgccggct
     6481 tcctgagccg cctcatccgg ccgcaggacc gctcgttcgt catcggcctc gccgcccgtc
     6541 cctacctcct cttcgctccg accaacgacg ccgcggcgat ggcgaaggcc agccttcccg
     6601 agctacgggc gcgcggcggg acggcgctct acgactcctt ggcgaccgct gtcctcctcc
     6661 tccgcgaggg ccggggccag cgcgccatcg tcctgctgtc ggacggcggc gactcgctga
     6721 gccatgccac cctgcagcag gcggtcgatc tcgcccggca gggcggagtg acgatctacg
     6781 cgatcggact gggcgtccgg acgcctgccg ccccgctcgt cgccgtcggt gccccccttt
     6841 cccacgagca gcaggcccgc cagctggagc gcatgaccga gcgccgcccg cgggccaggc
     6901 acacgccgct cgacgacctc gcccaggaga ccggcggccg ggtgttctac atcgacaagg
     6961 ccaaggagtt ggaggaggtc tacggccaga tcgaggagga tctgcgcagc cgctacctcc
     7021 tcgcctacca gacggagcgc ggggaggacc cgggggatcc ctctgtcgag gtgcgggtca
     7081 agaagcgcgg gttgaaggcc cggacggccc gcggttacta tccttgacgc cgcctcctgc
     7141 ctgcccggcg tcgcggcctc gcgaaaggaa acgagaatga cacgttacgg aatcatcggc
     7201 gtgtgctcgc tggcggcggc cctgggaacc ggggcgctgg ctccggcggc cgcgcagccg
     7261 cccgccccgg cgaccggcaa gccggccggg aagcccgcct ccaacccgat cgtcgagcgc
     7321 atcggcaacc gcgccttcat tcaggtggag tcgccgagct tcgccaacct gacgccgaag
     7381 cagaagctcc tcgcctacca cctgagccgg gcggcgatcc agctcgatcc cgtcttctat
     7441 gaccagatgt cggactacgg catcccggcc aagcgcctgc tgggcgccct ggtcgagcgc
     7501 cccgagcggc tgccggcggc catgcgccag ccggtcgtcg actacgccaa gcagttcttc
     7561 gccaacagcg gcaaccacga cggggcgacc ggccgcaagt tcctcccggc cttctccttc
     7621 gaggacttcg ccgaggccgc cgagcaggcg cgccagaagg gcgcccccct gggcaccaag
     7681 gaccagctca agaccgtgct cggcgagctg cgccagccgc tgttcgaccc caactaccag
     7741 gtctccgtca ccaacaagaa cccgccgccg ggcgaggaca tcctcaccgc ctccagcaac
     7801 aacctctacc agggggtgac gctggaggac ctcaaggggt tcaccgagcg gttccccctc
     7861 aactcgcggt tggtgaagaa ggacggcaag atcgtcgagg aggtctaccg cgccggcacc
     7921 cccgacggca aggtgccgcc cggcctctac gccagggagc tcaaggcggt catccgcgag
     7981 ctcgagcagg cgatccccta cgccgagccc gaccaggcca aggtgctcca ggcgctcatc
     8041 cgcttttacc agaccggcag cgccgaggac tgggcggcct acaacgtcct ctggatccgc
     8101 aacgatccgg aggtcgacat ggcctccggc ttcatcgagg tctaccgcga cgcccgcggc
     8161 gccaagggct cggcccagac cgtggtctcg gtgcgcgaca aggagctcgg gccgctgatg
     8221 cagaagctgg ccgccaacgc cgtctacttc gagggcaagg cgccgtgggc cgacaagtac
     8281 aagaagctgg acgtcgagcc gccggtgggc aaggcgatcg agaccgtcat cgccaccggc
     8341 gacttcacgg tcaacaccgt gggcgtcaac ctgcccaacg agcaggacat ccgcgagaag
     8401 cacggcacca agagcgtgct gctgaccaac gcctcgcggg cgctcaacgc cacccgcggc
     8461 gtcaagctgg cgaccgagtt ctccatggag cccgacgagc aggagctgtt cgagaagcac
     8521 ggcaccatgg cgggcgtcct ccacgtcgcc atgcacgagg tcatcggcca cggctccggc
     8581 aaggtggcgg tgccgaacga cccgcgcacc tacctgcgcg agtattactc gacgctcgag
     8641 gaggcgcgcg ccgacctggt ctcctactgg aacatctacg atcccaagct cgccgagctg
     8701 ggggtcgagg acgtccaggg ggtggccaag gagctctacc gggcgctcgc ccggcagggc
     8761 ctcaccaccc tcaaccgcta ccccacgggc gacgaggcgg aggaggacca cgaccgcaac
     8821 cgcctgctga tcgtcaacta cctcatcgag gccggggcgt tcgagcgcct ccagaaggac
     8881 ggccactggt acatcacggt caaggactac gacaaggcgc acgcgaccgt cggcaagctc
     8941 ctcgccgagc tgatgcgcat caaggccgag ggggactacg acgcgatcaa ggcgctggtc
     9001 gacaagtacg gcgtccactt cgacccggcg gtgcgcgacg acgtggtggc ccgcttcaag
     9061 aagctggaca tccccaccta ctacaccggc atctacccgg acttcgcgcc gaagaaggac
     9121 aagagcggca aggtgaccgc cgtcgaggtc tcctatccgc gggacttcct gcgccagcag
     9181 ctcgagtggg cgcgcttgaa cggcaccctc gggttcacgg cggacgggaa gcaggcgacg
     9241 aagtgacgcg cgcggtcccc tccgccgccc tcctgctcgc caccctcctg gtggcgggcg
     9301 gagccctgtc gcaggccgaa gcgccggggc agctccagtt cgcccgcctg aaccgcacct
     9361 acagcgacgt gatcacggag atcgagccga tccggcaggg gccggtaacg gtgcggctct
     9421 cgtcgccgcg gcaccagctc acggtgcgca accacctgct gcggctggag cccggaaccg
     9481 gggggagcca cagcgccgag ctgcgggtcg acttctcggg caaggggtgg ctggtggcgg
     9541 acgtcgacgt cgcgggcatg ggcgggcggc tggaggacga cgtcaccgtg ccggcgcagt
     9601 cgcagacggt ggaggggcgg gcgcgcgtcc gcaaggtggc cgaggggtac ctgatcaccc
     9661 ccgagcagct gccgaagagc gtccgcgtcc gcatccagag cggcgtcggc tcccgcatcg
     9721 ccggcttctg cgacagggcg gcggcgctgc tcttctccaa cgtcgactgc ggggggctga
     9781 accgggcgct gtccacggcg gtggtgccgc tgccggcggc gggggagagc tacctgctgg
     9841 agaacgccga gctgaccgcc gccgagcggc agcgcatcga ctcctacctg gcgcgggaga
     9901 gccgctgacc atgcgggcct cgcgcggcac tccggtcacg ctgctcctcc tggtagccat
     9961 cggcgccgtc ttcgtgctgg agacgctgcg cggcggctcg accaaccccg aggtgctgat
    10021 cgcgctgggg gcgaaccacc cggagctggt gctcaaccag ggggagtggt ggcggctggt
    10081 gaccagcatg ttcctccaca tcggcatcgc ccacctgctg ctgaacggct gggccctctt
    10141 ccagatcggc gccatcgtcg agtcctggat gg
//
LOCUS       DASPJI010000420         6844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_710867, whole genome shotgun sequence.
ACCESSION   DASPJI010000420 DASPJI010000000
VERSION     DASPJI010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710867"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1834
                     /locus_tag="VEL74_11270"
     CDS             <1..1834
                     /locus_tag="VEL74_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYG63153.1"
                     /translation="APTYMVFEPLTRRGFPWLHLVARLRPGVSVAQAGVEIETIGRRR
                     AAQQAEDQKDPVAQVVPAAAAAFAPDRQDALTRMSWILLGVVVAVLLIACAVVSGLLL
                     VRLERRQKETAMQVALGISRSRLCAELLVESLLLSALGAVAGLALSSFARRLLLTQIP
                     ADMPVATKAASSLADPRVLAFSAVACVVVGLLFGLAPALRAMRTAPGAVLKGQGPGAG
                     ASRRGLALRNAFVVLQVALSTVLLIAAGLLLRTLGKVSAIEPGFQPEGAVTASFDLAQ
                     QGYDEAAGGRFAQQLLERLQQSPGIRSAAIAQSVPVSPLSMSLSFQVAGYSPPDGQPL
                     VADIDAVTPGYFTTLGIPLLRGRDFTPQDTATSQPVVIISDTMARRFWSGKDPIGQRL
                     GDVAPEGALIVGVVADVQAKSLREPPEPMFYLPLAQFYAPRLSVVVRSSGDPRLVQRQ
                     LLDAVASLDRNLPLFDLQTLPERLAKALVRERTVAGLFSAFGLLALVLAVGGLYSVIS
                     HATELRTREFGIRIAMGSDAGGVQRLVLRQGLVIVLAGLFLGVLASLGLTSWLSSLLV
                     EVSATDPATFAAIPLLLIMAALLACLVPAFRATRVNPMLALRRE"
     gene            1839..>6844
                     /locus_tag="VEL74_11275"
     CDS             1839..>6844
                     /locus_tag="VEL74_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63154.1"
                     /translation="MVQLYSRSSFKDVLRTRSLEGEGRRVFTFLRDGEEASEALSYAE
                     LDLRARGIAARLQAVAAPGERALLLYPPGLEFIAAFCGCVYSGVLAVPTPPPRPNRPD
                     SRLQSIVADSAPRIVLTTRALLARAERIREQVPALRDALWIAYDELDDQEGDDWREPA
                     LGPETPAFLQYTSGSTSTPKGVLVSHGNLLHNQESIRRAFGQDESSVVVGWLPLYHDM
                     GLIGNVLQPLYAGASCVLMSPQSFLQRPARWLEAISRYRGTTSGGPNFAYDLCGRAGL
                     DLTGLDLGSWRVAFNGAEPVRADTLERFAAAFAPAGFRADAFYPCYGLAEATLFVAGE
                     APGRAPAVRAFDKAALERDLAVPGGDGAAQRLVGCGRSWLGQQVAIVRPETGERCAEG
                     QVGEIWVSGPSVARCYWNRAEETAETFGARLAGEGPWLRTGDLGFVDGGELFVSGRLK
                     DLVIIRGRNHYPQDIERTAELSHPGLRSGGAAAFSVDAGGEERLIIVQEVERHRGRET
                     EALSEAIRRAVAEEHEVQVYEVVLLRPGSLPKTSSGKVRRQACRAGYLAGTLAVVGRS
                     ALGGAPLVETMEAGASLRTREELLALPAAERRAAVLSFLEERAAALLGRRPEPGQPLT
                     GLGLDSLRAVELKIVIETALATPVPLAELLEGCGTGELADRIAAALAGAPTAEEELAA
                     ASRLLPMWPVAPDAGDHPLSVGQRSLWLLDRLAPQEAVYNIAVAARSETDLHAEALLA
                     ALLRLAERHGALRASFHEVDGEVVQRIRERPEVDFQVVDAAGWSGRSLAERLGDEAWR
                     PFDLTAGPLLRLRLFTRPAGPSVLLLAVHHIVADFGSLAVMSRELGALYRQETGGPAA
                     ALPPLALRYADFVRWQEERLSGPRGRILWDYWRHRLAGPPPDLDLPADRLRPPVQTYR
                     GGARALGLPRALAEGARSLARDQGSTLFATLLALWQAQLGRYAGQEDFTVGSPMAGRG
                     APELQGLVGYFVNPVALRAGLAGEPTVAELVARTGRTAVEALEHSDWPFALLVERLRP
                     VRDPARPPLFQVMLVMQGALPGDDAGLPAFALGEAGARLALGGVALESVRIEERRAQV
                     DLLLRVAVTDEGLGLSLEFNSDLFDGPTADRMLGHFRTLLDGAVAEPGRRAATLPLLT
                     AGERAQAVTEWNDTVCPASESCLHEMFEAQVRRIPESEALVAGEERLTYGELNARANR
                     LARHLRCLRVGPETRVGVGLERSADLVVALLAVLKAGGAYVPLDPSYPAERLAFLAAD
                     SGARLLIARGVVPGELAACGLPVLDLDRETFAAQSAEDLEPWAAPDNLAYLIYTSGST
                     GRPKAVAIEHRSPVALLLWAREVFTPGELAGVLASTSISFDLSVFELFAPLAWGGRVI
                     LARDALALPSLPARDEVTLVNTVPSAMAELLRGWALPPSFTVDLAGEPIPAALVEELG
                     RHPAIRRVFNLYGPSEDTTYSTWARLAPEEPNGAPIGRPVAGTRAHVVDRGGELLPAG
                     VPGELCLGGAGLARGYLGRPELTAERFVPDPFSGLSGARLYRTGDLSRRRPDGILEFL
                     GRFDYQVKVRGFRIELGEIETALASHPAVREAAVLAQEDEAGGRRLVAYLALAPGARV
                     APGELRDGLRQRLPEPMLPAAFVTLEALPRNANGKLDRRALPRPEGLQTATERSYTAP
                     R"
BASE COUNT          761 a         2443 c         2577 g         1063 t
ORIGIN      
        1 cgcgccgacc tacatggtgt tcgagcctct gacccgccgc ggctttccct ggctccatct
       61 ggtggcgcgt ctgcgcccgg gggtgtcggt cgcgcaggcc ggcgtggaga tcgagaccat
      121 cggccgccgg cgggcggcgc agcaggccga agaccagaag gacccggtcg cgcaggtggt
      181 gcccgcggcg gcggccgcct tcgctcccga ccggcaggac gcgctcaccc gcatgtcgtg
      241 gatcctgctg ggagtggtgg tggcagtcct gctgatcgcc tgcgcggtcg tctcggggct
      301 gttgctggtg cgcctggagc ggcgtcagaa ggagaccgcg atgcaggtcg cgctggggat
      361 ctcccgcagc cggctgtgcg ccgagctgct ggtggagagc ctgctgctct ccgcgctcgg
      421 cgcggtggcc ggcctcgcgc tgtcgagctt cgcgcgacgg ctgctgttga cgcagatccc
      481 ggccgacatg ccggtggcga ccaaggcggc ctcgtcgctc gccgatccgc gggtgctggc
      541 gttctccgcc gtcgcctgcg tggtggtcgg cctgctcttc gggctcgcgc cggcgttgcg
      601 cgccatgcgc accgcgcccg gggcggtgct caaggggcag gggccgggcg cgggggccag
      661 ccggcggggc ctggcgttgc gcaacgcctt cgtcgtcctc caggtggccc tctccaccgt
      721 gctgctgatc gccgccggcc tgctgttgcg cacgctcggc aaggtgagcg ccatcgagcc
      781 gggtttccaa ccggaaggcg cggtgacggc gtcgttcgat ctcgcccagc agggctacga
      841 cgaggccgcc ggcgggcgtt tcgcccagca gcttctcgag cgcctccagc agagccccgg
      901 catccgttcg gcggcgatcg cccagagcgt gccggtgagt cccttgtcga tgagcctgag
      961 cttccaggtg gcgggctatt cgccgccgga cgggcagccg ctcgtcgccg acatcgacgc
     1021 ggtgacgccg ggatacttca cgaccctggg catccccctg ctgcggggac gtgacttcac
     1081 gcctcaggac accgccacgt cgcagccggt ggtgatcatc agcgacacga tggcccggcg
     1141 cttctggtcg gggaaggacc cgatcggtca gcggctcggc gatgtggcgc cggaaggggc
     1201 cctgatcgtc ggcgtggtcg ccgacgtcca ggccaagagt ctgcgcgagc ccccggaacc
     1261 gatgttctac ctgccgctcg cgcagttcta cgcgccgcgc ctgtcggtcg tcgtccgctc
     1321 gtcgggcgat ccccgcctcg tccagcggca gctcctcgac gcggtggcga gcctcgaccg
     1381 gaatctgccg ctgttcgacc tccagacgtt gcccgagcgc ctcgccaagg ctctggtgcg
     1441 ggagcgcacg gtcgcgggcc tgttctccgc tttcggtctg ctcgccttgg tgctggccgt
     1501 cggcgggctg tacagcgtga tctcccatgc cacggagctg cgcacgcgcg agttcggtat
     1561 ccgcatcgcc atgggctcgg atgccggcgg ggtccagcgt ctggtgctgc ggcagggcct
     1621 ggtgatcgtg ctggccgggc tcttcctcgg ggtgctcgcg tcgctgggcc tgacctcatg
     1681 gctgtcgagt ctgttggtcg aggtcagcgc aaccgatccc gcgaccttcg ccgccattcc
     1741 cttgctgttg atcatggcgg ctctgctcgc ctgcctggtg cccgctttcc gggcgacccg
     1801 ggtcaatccg atgttggcgt tgcgacggga gtagacgttt ggttcagctg tactcccggt
     1861 cttcgttcaa agacgtcctg cgcacccgat ccctcgaggg ggaggggcgc cgcgtcttta
     1921 cctttctgcg cgacggcgaa gaggcctcgg aagccctctc ctatgccgag ctggacctgc
     1981 gggcgcgcgg gatcgccgcc cgcctccagg cggtcgccgc gcccggcgaa cgggccctgc
     2041 tgctgtaccc gccgggcctg gagttcatcg ccgccttctg cggctgtgtc tacagcggcg
     2101 tcctcgccgt tccgactccg ccgccgcgcc cgaaccgtcc cgactcgcgg ctccagtcga
     2161 tcgtcgccga ttcggcgccg cggatcgtgc tcacgacgcg cgccctgctc gcccgggccg
     2221 agcgcatccg cgagcaggtc ccggccctgc gcgacgcgct ctggatcgcc tacgacgagc
     2281 tcgatgacca ggagggggac gactggcgcg agcccgcgct gggaccggag acgccggcct
     2341 tcctgcaata cacctccggc tcgaccagca cgcccaaggg ggtgctggtc agccacggca
     2401 acctgctcca caaccaggag tcgatccgcc gcgccttcgg ccaggacgaa agctcggtgg
     2461 tggtcgggtg gctgccgctc taccacgaca tggggctgat cgggaacgtc ctccagcccc
     2521 tgtacgccgg cgcctcctgc gtcctgatgt cgccgcagtc cttcctgcag cggccggcgc
     2581 gctggctgga ggcgatctcg cgttaccgcg ggacgaccag cggcgggccg aacttcgcct
     2641 acgacctgtg cgggcgcgcc ggcctcgatc tgacgggtct cgatctgggg agctggcggg
     2701 tcgccttcaa cggcgccgag ccggtgcgcg ccgacaccct ggagcggttc gccgcggcct
     2761 tcgcgccggc cggtttccgg gctgatgcgt tctatccctg ttacgggctg gcggaggcga
     2821 ccctgttcgt tgccggcgag gcgccgggcc gggcgcccgc ggtgcgcgcc ttcgacaagg
     2881 cggccctgga aagggacctc gcggtgccgg gaggggatgg cgcggcacaa cggctcgtgg
     2941 gctgtggccg ttcctggctc ggccagcagg ttgccatcgt ccggcctgag accggcgaga
     3001 ggtgcgccga ggggcaggtg ggggagatct gggtcagcgg tccaagcgtc gcccggtgct
     3061 actggaaccg ggccgaagag accgctgaga cgttcggcgc ccggctcgcc ggcgaggggc
     3121 cctggctgcg caccggcgat ctcggcttcg tcgacggcgg cgagctgttc gtctccgggc
     3181 ggctcaagga tctggtcatc atccgcggcc gcaatcacta cccccaggac atcgagcgta
     3241 ccgccgagct cagccatccc gggttgcggt ccggcggcgc cgcggccttc tcggtcgacg
     3301 ccggcggcga ggagcggctg atcatcgtgc aggaggtcga gcggcaccgc ggccgggaga
     3361 ccgaagccct gagcgaggcg atccggcggg ccgtcgccga ggagcacgag gtccaggtct
     3421 acgaggtggt gctgctgcgg ccggggagcc tgccgaagac ctcgagcggc aaggtccggc
     3481 gccaggcctg ccgcgccggt tacctcgcgg ggaccctggc ggtggtgggg aggagcgcgc
     3541 tcggcggcgc gccgctggtg gagaccatgg aggccggcgc gtctctccgg acgcgcgagg
     3601 agctgctcgc cctgccggcg gcggagcggc gcgcggccgt cctctctttc ctcgaggagc
     3661 gcgccgccgc cctcctcggc cggcggccgg agcccggcca gccgctgacc ggcctggggc
     3721 tcgattcgct gcgggctgtc gagctcaaga tcgtgatcga gaccgcgctc gcgacaccgg
     3781 tgccgctcgc cgagctgctc gaagggtgcg gcacgggaga gctagcggac cggatcgcgg
     3841 ccgcgctggc aggcgctccc acggcggagg aggagctcgc cgcggcgagc cgactgttgc
     3901 caatgtggcc cgtggcgccg gatgccggcg atcacccgct ttcggtaggc cagcgctccc
     3961 tctggctgct ggaccggttg gcgccgcaag aggccgtcta caacatcgcg gtcgcggcgc
     4021 ggtcggagac cgatctccac gccgaagcgc tgctcgccgc cctgctgcgc ctcgccgagc
     4081 gccacggcgc cttgcgcgcc agcttccatg aggtggacgg ggaggtggtg caacggatcc
     4141 gggaacgccc cgaggtcgac ttccaggtgg tggacgccgc cggctggagc ggccggtcgc
     4201 tcgcggagcg gctcggcgat gaggcctggc ggccgttcga cctgacggcc gggccgctgc
     4261 tccggctgcg gctcttcacc cggccggccg gtccgtccgt gctgctcctg gccgtccacc
     4321 acatcgtggc ggacttcgga tcgctcgccg tgatgagccg cgagctgggg gcgctctacc
     4381 ggcaggagac gggggggccg gcggccgcgc tcccgccgct cgcgctgcgc tacgccgact
     4441 tcgtgcgctg gcaggaggag aggctgtccg gtcccagggg ccggatcctc tgggactact
     4501 ggcgtcaccg tctggcaggg cccccgcccg acctggacct gccggccgat cgtctccggc
     4561 cgcccgtcca gacctaccgc ggcggcgccc gagcgctggg gctgccgcgc gcgctggccg
     4621 agggcgcccg ctccctggca cgcgatcagg gatccacgct cttcgcgacg ctgctcgccc
     4681 tctggcaggc gcagctcggc cgctatgcgg ggcaggagga cttcacggtc ggctctccca
     4741 tggcgggccg cggcgcgccg gagctccagg ggctcgtcgg ctacttcgtc aacccggtgg
     4801 cgctgcgggc cggcctcgcc ggagagccga ccgtcgcgga gctcgtcgcc cggaccggcc
     4861 ggacggcggt cgaggcgctg gagcactccg actggccgtt cgcactgctc gtcgagcgtc
     4921 tgcggccggt gcgcgatccc gcccgtccgc cgctcttcca ggtgatgctc gtgatgcagg
     4981 gggcgctgcc gggggatgac gcggggctgc ccgccttcgc gctcggcgag gccggggcgc
     5041 gcctggcgct gggcggcgtg gcgctcgaat cggtgcggat cgaggagcgg cgggcccagg
     5101 tcgacctact gctgcgggtg gcggtgacgg acgaggggtt ggggctgagc ctcgagttca
     5161 actccgatct gttcgacggc ccgaccgcag accgcatgct gggccacttc cgtactctgc
     5221 tggacggggc ggtggccgag ccgggccggc gggccgccac cctgccgctg ctcaccgctg
     5281 gcgagcgtgc gcaggcggtc accgagtgga acgacaccgt ctgtcctgcg agcgagtcct
     5341 gtctgcacga gatgttcgag gcccaggtcc ggcgcatccc tgagtctgaa gcgctcgtcg
     5401 ccggagagga gcggctgacc tatggcgagc tcaatgcccg ggccaaccgg ctggcgcggc
     5461 acctgcggtg cctgcgggta gggccggaga cccgggtcgg cgtcgggctc gaacggtccg
     5521 ccgacctggt ggtggccctg ctcgccgtgc tcaaggcggg aggggcctac gtgccgctcg
     5581 acccctccta ccccgcggag cggctggcct tcctggccgc ggactccggg gcgcgcctgc
     5641 tgatcgcccg cggggtcgta ccgggggagc tcgcggcctg cggcctgccg gtgctcgatc
     5701 tcgatcgcga gaccttcgca gcccagagcg cggaagacct ggagccctgg gcggcgccgg
     5761 acaatctggc ctatctgatc tatacctcgg gctcgaccgg ccggccgaag gcggtggcga
     5821 tcgagcatcg cagcccggtc gccctgttgc tctgggcacg cgaggtcttc acgccggggg
     5881 agctcgcggg ggtcctcgcc tcgacgtcga tcagcttcga cctctccgtc ttcgagctct
     5941 tcgcgccgct cgcctggggg ggacgggtga tccttgcccg cgacgccctg gcgctgccgt
     6001 cgctcccggc ccgggacgag gtgacgctgg tcaacacggt cccctcggcg atggccgagt
     6061 tgctgcgggg gtgggccctg ccgccgtcgt tcacggtcga tctggcgggg gagccgatcc
     6121 ccgccgcgct ggtcgaggag ctcggccggc accccgcgat ccggcgggtc ttcaatctct
     6181 acggcccctc cgaggacacc acctactcga cctgggcgcg gctggcgccg gaggagccga
     6241 acggtgcgcc gatcggccgg ccggtcgccg gcacgcgcgc tcatgtcgtg gaccgcggcg
     6301 gcgagctgct gccggccggc gtgccgggcg agctctgcct gggtggggcg ggcctggcgc
     6361 gcggctatct cggccgcccg gagctgaccg ccgagcgctt cgtgcccgac ccgttctccg
     6421 gtctgtccgg agcccggctc tatcgcacgg gcgatctctc ccgtcgccgg ccggacggta
     6481 tcctcgagtt cctcgggagg ttcgattacc aggtcaaggt gcgcggtttc cggatcgagt
     6541 tgggggagat cgagacggcg ctcgcctccc atccggcggt gcgcgaggcg gcggtgctgg
     6601 cgcaggagga cgaggccggc ggccggcggc tggtcgccta tctggcgctg gcgccggggg
     6661 ctcgggtcgc gccgggggag ctgcgcgacg gcctgcggca gcggctgccc gagcccatgc
     6721 tgccggcggc cttcgtcacc ttggaggcgt tgccgcggaa cgccaacggc aagctcgacc
     6781 gccgggcgct gccacggccg gaggggctcc agaccgcgac ggagcgctcc tataccgcgc
     6841 cgcg
//
LOCUS       DASPJI010000421         9692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_428406, whole genome shotgun sequence.
ACCESSION   DASPJI010000421 DASPJI010000000
VERSION     DASPJI010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9692
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428406"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..882
                     /locus_tag="VEL74_11280"
     CDS             295..882
                     /locus_tag="VEL74_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63155.1"
                     /translation="MQDNLKGTTVPNPQHPNAFSSSFLNRLAERDEPPTAGDADVAGP
                     WRIEPVVSNGRHGFGLFRVGESLSRGFAPSAVFPSRWLALVCAAILPGTGRDPLLILS
                     RQPDAHGAYPVALAGGGSDGGGGDDDGEGGGGTAGFFPLFDERAVDAMNVLAHLLRSP
                     ESLAFVLEAAGSVALERCGAILDERVAADGAAGSE"
     gene            complement(987..1919)
                     /locus_tag="VEL74_11285"
     CDS             complement(987..1919)
                     /locus_tag="VEL74_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63156.1"
                     /translation="MARSSAQALVSPQRAGRRSQEGQPVQLDLFGPGTPALPAASIAR
                     ELPPPTSRPTLVPHPCDPEAELLRRLNRWNNGRLRSLTFTDNRRTILTVRPGAPGDSS
                     KLCLRIHRSFAVAPEEVLRAVATFVDSKRGSERARQALVVIREHFARHRQTEGRRRVP
                     LRPAGEAYDLRRIADDLNQRYFEGRLTAEITWGRGPAGVAHRCRRGARSRTSSLQLGS
                     YSYEDNLIRIHRLLDDPGIPRYVVEAVVYHELLHADMPPVIQNGRRLFHTPEFRRRER
                     QFRQLGRADEWIQENLPDLLQARAESARRSRRKA"
     gene            complement(2305..3279)
                     /locus_tag="VEL74_11290"
     CDS             complement(2305..3279)
                     /locus_tag="VEL74_11290"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYG63157.1"
                     /translation="MHSANKIPDPALLYAPHGEPGRFFCPWQPFNTGLLDVLRWKLKG
                     KGPYDRKTPPCVPVVENDGAYLKDGGAPPSVTWVGHSSFAIHDGGDVVLTDPHWGARA
                     LLPPRLSPPGIPLAAIPPGAFAVLSHNHYDHLDAWTVRQLPADLPWYVPLGLGTWFRS
                     RDRTRVTELDWWQSVRHGRWTLTFLPAQHWSNRIGMGRNASLWGSWMLDSGERRYYFA
                     GDSGSYHGFAEIGRRFPGIDVALLPIGAYEPRWFMRYQHVDPGEAYQAFLDLGARWMI
                     PMHWGAFDLTDEPVDLAPQVLRQALAERGADAGRVPILAVGERWVVGP"
     gene            3317..4648
                     /locus_tag="VEL74_11295"
     CDS             3317..4648
                     /locus_tag="VEL74_11295"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG63158.1"
                     /translation="MSLVEILQDHNPWWSDGAKRRAADYPIRRDLQPLLLAQVLRVED
                     RRAMVLVGPRQVGKTVLLFQLADDVLAAGWPPSNLTYFDFSDDRLTTPVTAREIVEAE
                     PVGLDPRYPRVFLFDEIRSAPEWDRWLKQAVDSKVGRIVATDSAAGLLREGGLESGQG
                     RWDQVLIEGLSFREFAKLHSLTGEAAEDVVHRLPNLHDLYVERGGFPEHATSRNPFEV
                     RRRLRADIAERAILRDVSGNVSDVQRVLDLFVYLVQDSGAELNADARGRDLGADPRSV
                     RDWVRRLTDTSLLCPLERLASRASAALRSKVKLYAADPGLVMSFALSPVRDPKVRARA
                     FEAAVFRHLRELARTEDCRLGYFRQGDDLEVDFVLEMGERRVAIEVTSSSRLRPEKLA
                     RLRKAGEILGVSRPLLIHGGLVEQKVEGVQPVALPSFLMNPGSCLQEGADE"
     gene            4641..5885
                     /locus_tag="VEL74_11300"
     CDS             4641..5885
                     /locus_tag="VEL74_11300"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG63159.1"
                     /translation="MSEEIPLWERESLQREFKDRLALDNPEIIAREAVGMLNASGGTV
                     WVGLRDDKEGRAVEVQPIPESEREKKRLLDFLVDAIEPSPRGRELDVRIVEREGGALL
                     TVVLHPSETRKPYALLRKGGRHFVTRVGDRIRPMTREEIASRFMGGGEEKEVVEETEK
                     SLRSELQLFQREQPDSEVLWLRLAPTARLSLDLNQLESSDLLIDPAATGNRRGGSTFL
                     AARAWGSLPGRARESGATSYLAVGKPGSSELTVHPDGSLRFLVPLKSLHAGADPEVSK
                     PLYPEALAEYPTSVFRLAAKLYAQPSLWEGGDVPPPDSILVVGLALLGLRGWSLRPGS
                     PHLPGSIGHHMFLASAPRVYQEDDFILEPLLHFKNRDVCEDPDSCGFRLVRRIYGDAF
                     LFSLKDLPAEFDRKTGRLVLPE"
     gene            complement(5892..6356)
                     /locus_tag="VEL74_11305"
     CDS             complement(5892..6356)
                     /locus_tag="VEL74_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYG63160.1"
                     /translation="MPSGSDRLKVHEHRTEILLPRSREEVFGFFADAGNLQAITPDWL
                     RFGILTPLPIEMRQGALIEYRLRLRGIPLRWKTGITAWDPPRRFVDEQLRGPYRLWVH
                     EHTFREADGGTLVGDYVRYAVLGGTLVNKLFVERDVRRIFEYRQERLRERFG"
     gene            complement(6343..6723)
                     /locus_tag="VEL74_11310"
     CDS             complement(6343..6723)
                     /locus_tag="VEL74_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015138210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG63161.1"
                     /translation="MIVLDTHAWVWWSSSPEKLSPRAKQLIRDAEEVGISAISPWEVS
                     MLVAKGRLRLDRDVLAWVEEALAAGISLLPISPRIAVAAGSLEELHGDPADRLIVATA
                     METGSLLISKDSGIRAFAGVNAVW"
     gene            complement(6720..6944)
                     /locus_tag="VEL74_11315"
     CDS             complement(6720..6944)
                     /locus_tag="VEL74_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYG63162.1"
                     /translation="MSIKARSVAAPEFESHCLSLLDEVAATGETLIVTKEGKPVAKVV
                     PFERPRPLLGSVLYEDDIVSPTGEKWDAEF"
     gene            complement(6976..7347)
                     /locus_tag="VEL74_11320"
     CDS             complement(6976..7347)
                     /locus_tag="VEL74_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HYG63163.1"
                     /translation="MPTSRLAPCPRTPNCVSTQAPPGPQHMDPIPYTGTRAEAQERLL
                     RVLRAHPRTRIVKQEPGYLHAECRSLIFRFVDDVELEIDEAARLIHFRSASRLGRKDF
                     GVNRARMEQIRAAFLAQTPRS"
     gene            7411..8517
                     /locus_tag="VEL74_11325"
     CDS             7411..8517
                     /locus_tag="VEL74_11325"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HYG63164.1"
                     /translation="MESIHDVPTPALVLDLDVLEANLRRMAAKTAALGVSLRPHTKTH
                     KCVEVAERQRALGAAGITVSTLYEARVFADHGFSDITWAFPVILSRLREVEELAARVT
                     LRLILDSPAAVDALEALRRPLHVWLEVDSGQHRSGVDPESPAALELARALAESPWLIF
                     DGLLTHAGHSYDGKSREEIAAVAEEERRVMAGLAERLRGEGIEVPAVSVGSTPGMSAR
                     PETARLDGVTEARPGNYVFYDYTQVALGSCRVVDCGVTVLASVVSHRPGDEQCVVDAG
                     ALAMSKDLGPRGHAGFGEVFADSGGGTGAGTLSGARLTSLSQEHGIVGAPFPVGTRLR
                     VLPNHSCLTVAQFDEYLVVRGDEVVDRWKIWRGR"
     gene            8552..>9692
                     /locus_tag="VEL74_11330"
     CDS             8552..>9692
                     /locus_tag="VEL74_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYG63165.1"
                     /translation="MLMLDAETRDALWRRLIEAIEDYASRVGEARVAPELDPEAIRSL
                     LAPFDFARPVEPLAALDFAVENLWRHQVHTPHPRYFGLFNPAPATMGIAGDALVAAFN
                     PQLAAWSHSPFANEVENHLLRCLGERLGYDPARVEGTFCSGGAEANHTALLTAITRAF
                     PEVGRRGLLALRSQPVVYATRETHDSIWKAARVSGLGTEAVHEVRIDAGRRMRADDLA
                     ARIREDREQGFAPFMVVATAGSTGAGVIDPLEELAEIADQERLWLHVDAAWGGGAALV
                     PELRSRLAGIGRASSITFDAHKWLSVPMGAGVYLTRHAGILERTFRVAAGYMPREASA
                     LPVADPWAHTIQWSRRFTGLKLFLSLAVAGWSGYEEAIRHQTAMGD"
BASE COUNT         1463 a         3214 c         3431 g         1584 t
ORIGIN      
        1 cgacagcgcc acccccgaca cggccgcgag gagaaggttc agcgtccccg ggcaaaaaat
       61 ttcctcctgc cctgttgaca cctctccgcc cctctcgata gactctggac atcagtacaa
      121 agcccggcgc gttgttcttc ccgggaggaa gggggatcga ggagaagagg aagaagaggc
      181 aggaccccac gacttgaacg gggcaggccg gcgcgtggct ggcttgcggc ccggaagtga
      241 gcttggaccc tcactcccag gcctatccca cacaacctcg acacaggagg atttatgcag
      301 gacaacctga agggtaccac tgtacccaat ccgcagcatc ccaacgcttt cagcagttcc
      361 ttcctcaacc gtctggccga gcgggacgag cctcccacgg ccggtgacgc cgacgtggcc
      421 ggaccgtggc ggatcgagcc cgtggtctcg aacgggcggc atggcttcgg cctgttccgg
      481 gtgggcgaga gcctctcccg cggcttcgcc ccgtcggcgg tcttcccgtc ccggtggctc
      541 gcgctcgtct gcgccgccat cctccccggc accggccggg accctctcct gatcctctcc
      601 aggcagccgg acgcccacgg cgcctatccc gtcgctctcg ccgggggtgg cagcgatggc
      661 ggaggcggcg atgatgacgg cgagggcggc ggcgggacgg ccgggttctt ccccctgttc
      721 gacgagcggg cggtcgatgc catgaacgtc ctcgctcatc tcctgcggtc gccggagtcg
      781 ctcgccttcg tgctggaggc ggccgggtcc gtggccctgg agcggtgcgg cgccatcctc
      841 gacgagcggg tggcggcgga cggggcggcc ggctccgagt aggacatcct catagatagg
      901 gggcgtcacc gccgggtcct tcggccggtg gcgcccctcc ttttccgcat ctcctgccgg
      961 agcggccctg tctggaggcg gagaggtcag gccttgcggc gggagcgccg ggcggactcg
     1021 gcgcgcgcct ggaggaggtc ggggagattc tcctggatcc actcgtcggc cctgccgagc
     1081 tgacggaact gccgctcgcg gcgccggaac tccggggtgt ggaagaggcg ccggccgttc
     1141 tggatcaccg gcggcatgtc cgcgtgcagc agctcgtggt agacgaccgc ctcgacgacg
     1201 tagcggggga tgccggggtc gtcgagcagc cggtggatgc ggatcaggtt gtcctcgtag
     1261 gagtagctgc cgagctgcag actggaggtg cggctgcggg cgccgcggcg gcagcgatgg
     1321 gcgacgccgg cggggccgcg gccccaggtg atctcggcgg tcagccgccc ctcgaagtag
     1381 cgctggttga ggtcgtcggc gatgcgccgc aggtcgtagg cctcgccggc cggacgcaac
     1441 ggcacgcggc ggcgcccctc ggtctggcgg tggcgggcga agtgctcgcg gatgaccacc
     1501 agcgcctggc gcgcccgctc gctgccgcgc ttgctgtcga cgaaggtggc gacggcgcgc
     1561 agcacctcct cgggggcgac cgcgaacgag cggtggatgc gcaggcagag cttgctcgag
     1621 tcccccggcg cgcccgggcg gacggtgagg atggtgcgcc ggttgtcggt gaaggtcagg
     1681 gagcggaggc ggccgttgtt ccagcggttg agccggcgga gcagctcggc ttccggatcg
     1741 caggggtggg ggacgagcgt ggggcggctg gtcggcggcg gcagctcccg ggcgatcgat
     1801 gccgccggca gcgccggggt cccgggcccg aacaggtcga gctggaccgg ctggccctct
     1861 tggctgcggc gccccgcccg ctggggagac acgagagctt gtgcggaaga tctcgccatc
     1921 cgtgatcctc tcgtcatccg gtcccatggg cccggctcct gacgacagcc cgaagacttt
     1981 agcacaggga gccggggcct ccggtgctct ccgtcctcct cgaaacggcc ctcgtttcgg
     2041 gagaaacggc cgtcctctcg cgcgagacgg cagccattcc ctccgtaagg gccttcgttt
     2101 ctcgcgaaat gcccgccatt cctcgagaaa cggcgatcat ttcgcacgaa aggtcggccg
     2161 tttccaggga aatggccgcc atttctcgag gaatgaagag cattcctgcc ggggcagcgg
     2221 ctccccccgg aaggatggtt gtcattccgg cgggaatggc gatcgtttcg gctgcccgaa
     2281 gtctgtttcc gcggcttgcc tgctttacgg acctaccacc cagcgttccc ccaccgccag
     2341 gatcgggacc cggcccgcgt ccgccccgcg ctcggccagc gcctgccgga ggacctgggg
     2401 ggcgagatcc acgggctcgt cggtgaggtc gaacgccccc caatgcatgg ggatcatcca
     2461 gcgcgcgccg aggtcgagga acgcctggta ggcctcgccg gggtccacgt gctgatagcg
     2521 catgaaccac cgcggctcat aggcgccgat ggggaggagg gcgacgtcga tgccggggaa
     2581 gcggcggccg atctcggcga agccgtggta ggagcccgag tcgccggcga agtagtagcg
     2641 gcgctcgccg gagtcgagca tccaggagcc ccagagcgag gcgttcctcc ccatgccgat
     2701 gcggttggac cagtgctggg cgggaaggaa ggtgagggtc caccggccat gccggacgct
     2761 ctgccaccag tcgagctcgg tgacgcgggt ccggtcgcgg gaccggaacc acgtcccgag
     2821 ccccagcggc acgtaccagg ggaggtccgc cggcagttgc cggaccgtcc aggcgtccag
     2881 gtggtcgtag tggttgtgcg acagcaccgc gaacgccccc ggagggatcg ccgccagggg
     2941 gatgcccggc gggctgagcc gtggagggag cagagcccgc gccccccagt ggggatcggt
     3001 gagcaccacg tcgccgccgt cgtggatggc gaacgacgag tgccccaccc aggtgaccga
     3061 cggcggcgcc cctccatcct ttagataggc gccgtcgttt tccaccaccg gcacgcaggg
     3121 gggcgtcttg cggtcatacg gccccttgcc cttgagcttc cagcgcagga cgtccagcag
     3181 cccggtgttg aacggctgcc acggacagaa gaaccgaccc ggctcgccgt ggggggcgta
     3241 gaggagggcg gggtcaggaa tcttgtttgc ggagtgcatt ggagtgccgc tagaatgaga
     3301 ggcgaggtgc ggtgggatga gccttgtcga gattcttcag gaccataacc cctggtggag
     3361 cgatggcgcc aagcgacggg cggcagatta tcccatccgc cgggatctgc agccgctgct
     3421 ccttgcgcag gtgcttcggg tggaggaccg ccgggctatg gtcctggtcg ggcctcgcca
     3481 agtgggaaag actgtgctcc tgttccaact cgcggacgat gtgctcgccg ccggctggcc
     3541 accttcgaac ctgacctact tcgatttctc cgatgaccgt ttgacgacac ccgtgaccgc
     3601 gcgggagatc gtcgaggcgg agccggtagg gctggacccc aggtatccaa gggtgtttct
     3661 tttcgatgag atccgctcgg ctccggagtg ggaccgctgg ctcaagcaag cggtggattc
     3721 caaagtgggt cgtatcgtgg cgacggattc tgccgccggt ctactacggg aggggggcct
     3781 cgagtccgga caggggcgtt gggaccaggt gctcatcgaa gggctgtcct ttcgagaatt
     3841 tgcaaagctc cactccctga ctggcgaagc agcggaggac gttgtccatc ggcttcccaa
     3901 cctgcacgac ctctatgtgg agcgaggggg ctttccagag cacgcgacga gcaggaatcc
     3961 gttcgaggtg cggcgacggc tacgggccga catcgcggaa cgggcgatcc tgcgggacgt
     4021 gtccgggaat gtctcggacg tacagagggt gctcgatctc ttcgtctatc tggtccagga
     4081 ctccggagcg gagctgaacg ccgacgctcg cggacgggat ctgggtgccg acccgcggtc
     4141 ggtacgggat tgggtacggc gcctcacgga tacatcgctc ctctgtcctc tggagcgcct
     4201 tgcttcccgg gcctccgcgg ccttgcgttc gaaggtcaag ctctatgccg ccgacccggg
     4261 tctcgtcatg tcgttcgcac tctccccggt gcgggatccg aaggtgcggg cacgggcttt
     4321 cgaagccgcc gtctttaggc atcttcgcga gcttgcccgc accgaagatt gccggctcgg
     4381 ctacttccgg cagggggacg atctggaagt cgatttcgtg ctcgagatgg gggagaggcg
     4441 ggtggccatt gaggtgacga gcagctcacg gctgcgcccg gagaagctgg cgcgtctacg
     4501 aaaggccgga gagatcctcg gggtgagccg tccgctgctc attcatggag ggctggtgga
     4561 gcagaaggtg gagggggtcc agcccgtggc gcttccgagt tttctgatga accccgggtc
     4621 ctgcttgcag gagggcgctg atgagtgagg agatccccct ctgggaaaga gagagcctcc
     4681 agcgcgagtt caaggaccgc ttggcgctcg acaaccccga gatcatcgcg cgtgaggccg
     4741 tcggcatgct caacgcttcg ggaggaacgg tgtgggtcgg cctcagggac gacaaggagg
     4801 ggcgcgcggt cgaggttcag cccatccccg agtcagaacg ggaaaagaaa cgcctgctgg
     4861 atttcctggt cgatgccatc gagccgtccc caaggggaag ggagctggat gtccggatcg
     4921 ttgagcgaga gggaggtgct ctcctcacgg tcgtcctgca cccgagcgag acccggaagc
     4981 cctatgccct ccttaggaag ggaggccgcc acttcgtcac gcgcgtggga gaccggatca
     5041 ggccgatgac cagagaagag atcgcgtcgc gattcatggg gggcggcgaa gagaaagaag
     5101 tcgtcgaaga gaccgagaag agtctgaggt cggagctcca gctcttccag agagagcagc
     5161 cagactctga ggtcttgtgg ctccgcctgg caccgacggc tcggctcagc ctcgacctca
     5221 accagctcga gtccagcgat ctcttgatcg accctgcagc gaccggaaat cgcagggggg
     5281 ggagtacttt cctcgctgcc cgggcttggg gctcgctgcc cggcagggct cgggagtctg
     5341 gcgccacctc ataccttgct gtgggcaagc ccggctcttc cgagctgacc gtccatccgg
     5401 atggcagctt gcgctttttg gttcccctca agagccttca tgcgggtgcc gatcccgagg
     5461 tttcgaaacc cctctatccc gaagcactgg ccgagtatcc gacctccgtt tttcggctgg
     5521 ccgccaagct ctatgcccaa ccctctctct gggagggtgg agacgtgcca ccgcctgact
     5581 cgatcctggt ggttggcctc gctcttctgg gtctccgggg ttggtcattg cgaccgggct
     5641 ctccgcatct cccgggatct atcggccatc acatgttcct ggcatctgct cctcgggtgt
     5701 atcaggaaga cgacttcatt ctcgagcctc ttctgcactt caagaataga gatgtctgtg
     5761 aggatccgga ttcctgtggt ttccggctgg tacgtcgtat ctatggagac gcctttctct
     5821 tctcgctcaa agatctcccc gcggagttcg accggaagac gggccgcctg gtccttcccg
     5881 agtaggctcc tctacccgaa cctctcccgc aaccgctcct gcctgtactc gaagatccgc
     5941 cgcacgtccc tctccacgaa aagcttgttg accagcgttc ccccgaggac ggcatagcgg
     6001 acgtagtcgc cgaccagggt gccgccgtcc gcctcccgga aggtgtgctc gtggacccag
     6061 agacggtagg ggccgcggag ctgctcgtcg acgaagcggc gcggcgggtc ccaggcggtg
     6121 atcccggtct tccagcgcag ggggatgccg cgcaggcgga gccggtactc gatgagcgcc
     6181 ccctgccgca tctcgatggg cagaggggtc aggatcccga agcgcagcca gtccggggtg
     6241 atcgcctgga ggttgcccgc gtcggcgaag aagccgaaga cctcctccct ggaccggggg
     6301 aggaggatct cggtgcggtg ctcgtggacc ttcaagcggt cgctaccaga cggcattcac
     6361 cccggcaaag gctcgaatgc cactatcctt cgagatcagg agcgagcccg tctccatggc
     6421 tgtcgcgacg atcaagcggt ccgcgggatc accgtgaagc tcttccaggg agccggcggc
     6481 gactgcgata cgaggagaga tcggtagaag ggagatcccg gcagccagtg cctcttccac
     6541 ccaggccagt acgtcccgat ccaaccggag gcgtcccttc gcgaccaaca tggagacctc
     6601 ccaagggctg atcgccgaga tacccacctc ttcagcatcc cggatcagtt gcttggcacg
     6661 aggggagagc ttctcaggag aagagctcca ccacacccag gcgtgggtgt cgaggacgat
     6721 caaaactcgg catcccactt ctcccctgtg ggggagacga tgtcgtcctc atacagcacg
     6781 cttcccagca gcggcctggg cctctcgaac gggaccacct tggccaccgg cttgccttcc
     6841 ttggtcacga tgagggtctc accggttgcc gcaacctcgt ccagcaggga gaggcagtgg
     6901 gactcgaact cgggagcggc cacggaacgc gccttgatcg acatggcttg tgcttacagg
     6961 aaccgcgagc ctctgtcaag aacgaggcgt ctgtgccaga aacgccgccc ggatctgctc
     7021 catccgtgcc cggttgaccc cgaagtcctt ccttcccagg cgcgaggccg accggaagtg
     7081 gatcagccgc gccgcttcgt cgatctccag ctccacgtcg tcgacgaaac ggaagatcag
     7141 ggaacggcac tcggcatgaa ggtagccggg ctcctgcttc acgatgcgcg tccgggggtg
     7201 cgcccggagc acccgcagga ggcgctcctg ggcctccgcc cgggtgccgg tgtaggggat
     7261 cgggtccatg tgctgggggc ccggcggcgc ctgggtcgag acgcagttcg gggtgcgcgg
     7321 gcagggggcg aggcgtgagg tgggcatagg gtctgtctcc ggtcggcttc gtattctatg
     7381 atgtgccccc ttctgcccga ggccctagcc atggagtcca tccacgacgt ccccaccccc
     7441 gcgctggtcc tggacctcga cgtgctcgag gccaacctgc gccgcatggc ggcgaagacc
     7501 gcggcgctcg gcgtctccct gcgcccccac accaagaccc acaagtgcgt cgaggtggcg
     7561 gagcggcagc gcgccctggg agcggccggc attacggtct ccaccctgta cgaggcccgc
     7621 gtcttcgcgg atcatggctt ctcggacatc acctgggcct tcccggtgat cctctcgcgc
     7681 ctgcgggagg tggaggagtt ggcggcgcgg gtcaccctgc gcctgatcct cgactcgccc
     7741 gccgccgtcg acgccctgga ggccctccgc cggccgctgc acgtctggct ggaggtcgac
     7801 tcggggcagc accgctcggg cgtcgacccc gagtcgccgg cggctctgga gctggcgcgg
     7861 gcgctggcgg agtccccctg gctcatcttc gacggcctcc tcacccacgc cggccactcc
     7921 tacgacggca agagccggga ggagatcgcc gccgtcgccg aggaggagcg gcgggtcatg
     7981 gccggcctcg ccgagcggct gcgcggggag gggatcgagg tgccggcggt gagcgtcggc
     8041 tcgacgcccg ggatgtccgc ccggccggag acggcgcggc tcgacggcgt caccgaggcg
     8101 cggcccggca actacgtctt ctatgactac acccaggtcg ccctcggctc gtgccgcgtc
     8161 gtggactgcg gggtgaccgt gctcgccagc gtcgtctccc accggccggg ggacgagcag
     8221 tgcgtggtgg acgccggggc gctcgctatg tccaaggacc tcggccccag ggggcacgcc
     8281 gggttcggcg aggtgttcgc ggattccgga gggggtaccg gcgcgggcac cctgtccgga
     8341 gcgcggctca cttcgctgtc acaggagcac ggtatcgtgg gagcgccgtt ccccgtgggg
     8401 acccgcctgc gcgtcctgcc gaaccactcc tgtctcacgg tggcgcagtt cgacgagtac
     8461 ctggtggtcc gcggcgacga ggtcgtggac cgttggaaga tctggcgcgg ccgctagcct
     8521 tcgctagcac ccggccgagg agagaagcgc gatgttgatg ctggacgccg agacccggga
     8581 cgccctgtgg cgccgcctga tcgaggccat cgaagactat gcctcccgtg tgggcgaggc
     8641 ccgtgtcgcc ccggagctcg accccgaggc gatccggtcg ctcctcgctc ccttcgactt
     8701 cgcgcgtccc gtagagcccc tggccgccct cgacttcgcg gtggagaacc tctggcgcca
     8761 ccaggtgcac accccccatc cgcgctactt cgggctgttc aaccccgccc cggccaccat
     8821 ggggatcgcc ggcgacgccc tagtggcggc gttcaacccg cagctcgcgg cctggagcca
     8881 cagcccgttc gccaacgagg tcgagaatca tctgctccgt tgcctgggcg agcgcctggg
     8941 gtacgacccg gcacgcgtcg aggggacctt ctgctcgggc ggcgccgagg ccaaccacac
     9001 ggcgctcctc accgccatca cccgcgcctt ccccgaggtc ggccgccgcg ggctcctcgc
     9061 cctgcgcagc cagccggtgg tctacgcgac gcgcgagacc cacgactcca tctggaaggc
     9121 ggcgcgggtg tcggggctcg gcaccgaggc ggtgcacgag gtgcgcatcg acgccggccg
     9181 gcggatgcgg gccgacgacc tggcggcgcg catccgggag gaccgggagc aggggttcgc
     9241 cccgttcatg gtggtggcca cggcagggtc gaccggcgcc ggggtgatcg acccgctgga
     9301 ggagctggcg gagatcgccg accaggagcg gctctggctg cacgtggacg ccgcctgggg
     9361 cggcggcgcg gcgctggtgc cggagctgcg gtcgcgcctc gccggcatcg ggcgcgccag
     9421 ctccatcacc ttcgacgccc acaagtggct gtccgtcccc atgggcgccg gcgtctacct
     9481 gacccgtcac gcgggcatcc tggagcggac gttccgggtg gccgccggct acatgccgcg
     9541 ggaggcgtcg gcgctgccgg tggccgaccc ctgggcgcac acgatccagt ggtcgcgccg
     9601 gttcaccggc ctcaagctct tcctgtcgct ggcggtggcg ggctggagcg gctacgagga
     9661 ggcgatccgt caccagaccg cgatggggga cc
//
LOCUS       DASPJI010000422         4649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_197387, whole genome shotgun sequence.
ACCESSION   DASPJI010000422 DASPJI010000000
VERSION     DASPJI010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197387"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VEL74_11335"
     CDS             complement(<1..431)
                     /locus_tag="VEL74_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018962499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HYG63166.1"
                     /translation="MPMQPGLIDLRSDTVTRPDAEMRRAMAEAEVGDDVWGEDPTAIR
                     LEEEAAAALGHEAALFLPSGTMGNQIALHLHARPGTEVICEARSHIFLYEMGAPAALS
                     GLILRTLPSAPGAEGQLDPGAVEAAILPPSPFKAATGLIVL"
     gene            complement(447..1190)
                     /locus_tag="VEL74_11340"
     CDS             complement(447..1190)
                     /locus_tag="VEL74_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63167.1"
                     /translation="MTDLNAPRTAWRLAALLLLLVSVAGPAAAWDLAADPPRRDFEAF
                     HRRLSGDVYHYPRHGAAPLGLTGFDVYADASVDRGFDEEPFYGTVVSGDLPGGLLSFA
                     RVGARKGLPGGVDIGAAYGRALGGDLKLVSADLQWAMIKGGTLSPALSLRLTGSRTVD
                     ADRFELDQYGAELLLSKGFAVLTPYIGGGVIYSDARLERELLPALEDDHTQSIFYAGL
                     TLNLLLPKITVEVEKAEEIQGAVRVAFGF"
     gene            complement(1183..2283)
                     /locus_tag="VEL74_11345"
     CDS             complement(1183..2283)
                     /locus_tag="VEL74_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63168.1"
                     /translation="MRAQSVPENEAPRPGVRLPEPGRFWIRYVPRLWEPPEMPWLDFA
                     EGKLGPWGRGKGRHLEPADLHQLAFPLDDLLYLPPVPEHLEAARREVAEKFLAAGTPV
                     LRQVFPGDELPANGDAEDSAETVEEFPYLPRPATPEFVFDLLPALLELDFTRLARLPS
                     GAAVVWPLLPGLTDSPSLWEEGCEVLAAAGVRHVQALAPALTPQDRRRLAERWGDEAT
                     FDALFHHAPPSEREFACTANGFGLFPFLPRPLPRPPIRGASNRRIAGVLALAAELWLR
                     LGRPVEQGQAIYRAARWIDSANYDLKGLARERNLAVLTPLDALSRKLIAELFESGEPP
                     LLRELLTEYVSPELSGGWADSGDTGTQEDSDD"
     gene            complement(2280..2930)
                     /locus_tag="VEL74_11350"
     CDS             complement(2280..2930)
                     /locus_tag="VEL74_11350"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HYG63169.1"
                     /translation="MFTGIVAERGRLVADPAPSGQGGVRLVIEHSQNLGKRLEIGASL
                     AVSGVCLTVTGLEPETGEPPIRSTVELSPETLARTNLGDLRGGGFVNLEPALRAGDAL
                     GGHWVQGHVDATARVVDRRDLSDHRVLAFEIPAGMEAYLVSKGSVTIDGVSLTVSALH
                     PGRFEVALIPHTLEVTTLDARVPGDRVNLEVDVLAKYVERILAVRGLVHEPAVSLP"
     gene            3039..3428
                     /locus_tag="VEL74_11355"
     CDS             3039..3428
                     /locus_tag="VEL74_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009480395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYG63170.1"
                     /translation="MSHRLNISSGGPWEARVGYSRAVRVGQHVHVAGTTAMTPEGLVG
                     KGDLYAQAKQTFATIEAALREAGAGLEHVVRTRTYVTDISRWEEAGRVHGELFGDIRP
                     ASTMVEVSRLIDPEMLVEIEVDAYVPE"
     gene            3474..3701
                     /locus_tag="VEL74_11360"
     CDS             3474..3701
                     /locus_tag="VEL74_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006618693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HYG63171.1"
                     /translation="MKQTHYLTAVIEREDDGYIALCPELDIASQGDTVEEAKENLREA
                     VELFFECADPSEVERRLHPEVFVTSLEIRVG"
     gene            3694..3918
                     /locus_tag="VEL74_11365"
                     /pseudo
     CDS             3694..3918
                     /locus_tag="VEL74_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            4007..>4649
                     /locus_tag="VEL74_11370"
     CDS             4007..>4649
                     /locus_tag="VEL74_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63172.1"
                     /translation="MRSRTVCGFFMLLLVLGLVPVSADSVIQRGIDVFTTPGDGKTFY
                     DFAKSPIPAGFFCKGSKPFAGKVAFKGLPLATGSPDQLWGADTVIERLDDAAFDEKGV
                     AMTRLQFRALSLVSLSPVKTSCGAYHVYVSLGGRQRITEMSILRTQEGGGNFLAPLAV
                     DVRMTFIPVKPGLGKAARKLELAEGFTFPPSPLPWSFRDRAQAKGIGLVSVDTD"
BASE COUNT          718 a         1583 c         1681 g          667 t
ORIGIN      
        1 tccagcacga tcagcccggt ggccgccttg aacggcgacg gagggaggat cgcggcctcc
       61 accgcccccg ggtcgagctg cccctcggcg ccgggagcgg aggggagggt gcggaggatc
      121 aggccggaga gcgccgccgg cgctcccatc tcatagagga agatgtggct gcgcgcctcg
      181 cagatcacct cggtgccggg gcgggcgtgg aggtggaggg cgatctggtt gcccatggtg
      241 cccgagggga ggaagagcgc cgcctcgtgc ccgagggccg ccgcggcctc ctcctccagg
      301 cggatcgccg tggggtcctc gccccagacg tcgtcgccga cctcggcctc ggccatggcg
      361 cggcgcatct cggcgtcggg ccgggtgacg gtgtcggatc gcaggtcgat caggccgggc
      421 tgcatgggca tctcctcact ctctcctcag aagccgaaag ccacccggac ggcgccctgg
      481 atctcctcgg ccttctccac ttcgacggtg atcttgggca gcaggaggtt cagggtcaac
      541 ccggcgtaga agatgctctg ggtgtggtcg tcctccagag cgggcaggag ctcgcgctcg
      601 agccgggcat cgctgtagat cacgccgccg ccgatgtagg gggtgaggac ggcgaagccc
      661 ttggagagca gcagctcggc gccgtactgg tccagctcga agcggtcggc gtccacggtg
      721 cgggagccgg tcaggcggag cgacagggcc ggcgagaggg tgccgccctt gatcatcgcc
      781 cactgcaggt cggccgagac gagcttgagg tcgccgccga gtgcgcggcc gtaggcggcg
      841 ccgatgtcca cgccgccggg gagccccttc ctggcgccga cgcgggcaaa ggagaggagg
      901 ccaccgggca ggtcgccgct cacgaccgtc ccgtagaagg gctcctcgtc gaagccgcgg
      961 tctacggagg cgtcggcata gacgtcgaag ccggtgaggc cgagaggggc ggcgccgtgc
     1021 cgggggtagt ggtagacgtc gccgctcaag cgccggtgga aggcctcgaa gtccctgcgc
     1081 ggcggatcgg cggcgaggtc ccaggcggcg gcggggccgg ctaccgaaac caggaggagc
     1141 agcagggcgg ccagtcgcca agccgtccgc ggcgcattga gatcagtcat cggaatcctc
     1201 ctgcgtcccg gtgtccccgg agtccgccca tcctccgctc agctcgggcg acacgtactc
     1261 ggtgaggagc tcgcggagga gcggcggctc tcccgactcg aagagctcgg cgatcagctt
     1321 gcggctcagc gcgtccagcg gggtgagcac ggcgaggttg cgctcgcggg ccagcccctt
     1381 gaggtcgtag ttggcgctgt cgatccagcg cgcggcgcgg tagatggcct gcccctgctc
     1441 caccgggcgt cccaggcgga gccacagctc ggcggccagg gcgaggacgc ccgcgatccg
     1501 ccggttggag gcgccgcgga tcggcggccg gggcagcggg cgcggcagga aggggaacag
     1561 gccgaagccg ttggccgtgc aggcgaactc ccgttccgac ggcggcgcgt ggtggaagag
     1621 ggcgtcgaac gtggcctcgt caccccagcg ctcggcgagc cgccggcggt cctggggggt
     1681 gagcgccggg gcaagcgcct ggacgtggcg cacgccggcc gcggccagca cctcgcaccc
     1741 ctcctcccag agcgacggcg aatcggtgag cccgggcagc agcggccaga cgacggcggc
     1801 gccgctcggc aaccgggcca gccgcgtgaa gtcgagctcc aggagcgccg gcaggaggtc
     1861 gaagacgaac tccggcgtcg ccggccgggg gaggtacggg aactcctcca ccgtctcggc
     1921 ggaatcctcg gcgtcgccgt tcgccggcag ctcgtcgccc gggaacacct gccgcagcac
     1981 cggtgtaccg gcggccagga acttctccgc tacctcgcgg cgcgccgcct cgaggtgctc
     2041 cggcacgggc ggcaggtaga ggaggtcgtc gagcgggaac gcgagctgat ggaggtccgc
     2101 cggctccagg tgccgcccct tgccgcggcc ccaagggccc agcttcccct cggcgaagtc
     2161 cagccagggc atctcgggcg gctcccagag ccgcgggaca tagcggatcc agaaccggcc
     2221 cggctccggc agacggactc ccggccgggg cgcctcgttc tcgggtacgg actgagctct
     2281 cacggcaggc tgacggccgg ctcgtggacg agcccgcgca cggcaaggat ccgctccacg
     2341 tacttggcga ggacgtccac ctcgaggttg acccggtcgc cggggacacg ggcgtccagg
     2401 gtggtgacct cgagggtatg ggggatgagc gccacctcga aacggccggg gtggagggcg
     2461 gacacggtaa ggctcacacc atcgatggtg accgagccct tggaaacgag ataggcctcc
     2521 atgccggcag gaatctcgaa ggcgagcacc cggtggtcgg agaggtcacg gcggtcgacc
     2581 acgcgggcgg tggcgtcgac atgcccctgg acccagtggc cgcccagggc gtcgccggcg
     2641 cgcagggcgg gctccaggtt gacgaagccg ccgccgcgca ggtcgccgag gttggtgcgg
     2701 gccagcgtct cgggggacag ctcgaccgtg gaccggatcg gcggctcgcc ggtctccggc
     2761 tccaagccgg tgacggtgag gcagactccg gacacggcga ggctggcgcc gatctccagg
     2821 cgctttccta ggttctggct gtgctcgatg accagccgga ccccgccctg tcccgacggc
     2881 gccgggtctg cgacgagccg tccccgctcc gcaacgatcc ccgtgaacat tcgtcctccc
     2941 taagatcccg cggagattat cccgattctg gagcgccggg tccacgcccg ggtgtcacct
     3001 gaggtagcct acgcgccgag atccaaggag aagacaccat gtcccaccgg ctcaacatct
     3061 ccagcggcgg cccctgggag gccagggtcg gctactcgcg ggccgtgcgc gtcggccagc
     3121 acgtccacgt ggcgggcacc accgccatga cccccgaagg gctggtcggc aagggggacc
     3181 tctacgccca ggcgaagcag accttcgcga ccatcgaggc ggcgctccgc gaggccggcg
     3241 ccgggctgga gcacgtcgtc cggacgcgta cctacgtgac cgacatcagc cgctgggagg
     3301 aggccggccg ggtccacggc gagctgttcg gggacatccg gcccgcctcc acgatggtcg
     3361 aggtgagccg gctcatcgat cccgagatgc tggtggagat cgaggtggac gcgtacgtgc
     3421 cggagtagga tgcgatcacc gagtgctacg atgctgctcc tgtggagatt cctatgaagc
     3481 agacccacta cctcaccgcc gtcatcgaac gagaagacga tggctacatc gccctatgcc
     3541 ccgagctcga catcgcgagc cagggcgata ccgtcgaaga agccaaagag aatctccgcg
     3601 aggcggtgga gctcttcttc gaatgtgccg atccctcgga agttgagcgc cggctgcatc
     3661 ccgaagtgtt cgtcacgagc ctggagatcc gggttgggta gactgcgggt gaggtccggc
     3721 cgggaagcct gccggattct cgtacaccac gggtttgtgg agatccgatg acgtggaagc
     3781 cacgtcatca tgcagaagaa ggtccccaac tctaccttga cggtcccggt gcctgatcac
     3841 gacgagctca ggcaggggac actgctttcc atcatccgcc agtcgggcct cccgcggagt
     3901 ctcttcgagg cgccctagct ccggcgcgcc aatctcgcac tcacgcacct acccaagcat
     3961 aggccggctc actatctggt cgtccttatg gtcaaggagg ttgtgcgtga gaagcaggac
     4021 cgtctgcgga tttttcatgt tgttgctcgt gctcggcctg gtccccgtgt ccgctgattc
     4081 cgtgatccag cgcgggatcg acgtcttcac cacccccggg gatgggaaga ccttctacga
     4141 cttcgccaag agccccattc cggccggctt cttctgcaag ggctccaagc cgttcgccgg
     4201 caaggtggcc ttcaagggcc tgccgctcgc caccggcagc ccggatcagc tttggggcgc
     4261 cgacacggtc atcgagcggc tcgacgacgc cgccttcgat gagaagggcg tcgccatgac
     4321 ccggctccag ttccgggcgc tgtcgctggt gagcctcagc ccggtcaaga cgtcctgcgg
     4381 cgcctaccac gtctatgtct ccctcggcgg ccggcagcgg atcaccgaga tgagcatcct
     4441 ccggacccag gagggcggcg gcaacttcct cgcgccgctc gcggtggacg tgcgcatgac
     4501 cttcatcccg gtgaagccgg ggctcggcaa ggccgctcgc aagctggagc tcgccgaggg
     4561 cttcaccttc ccgcccagcc ccctgccgtg gagcttccgg gaccgtgctc aggccaaggg
     4621 gatcggcctc gtctccgtcg acaccgacg
//
LOCUS       DASPJI010000423         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_537963, whole genome shotgun sequence.
ACCESSION   DASPJI010000423 DASPJI010000000
VERSION     DASPJI010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537963"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VEL74_11375"
     CDS             complement(<1..506)
                     /locus_tag="VEL74_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HYG63173.1"
                     /translation="MSEPAWMIYGVTGYTGELAAREAVRRGQRPVLAGRDPEKVGAIA
                     RELGLESRAFSLDDPQALAAGLAGLSAVLHCAGPFVRTSAPMVHACLAAGVHYLDITG
                     EIPVFEEVLSRGEEARRANVALLPGVGFDVVPSDCLAARLAAALPDATDLTLAFYNDR
                     GTLSRGTM"
     gene            complement(499..1656)
                     /locus_tag="VEL74_11380"
     CDS             complement(499..1656)
                     /locus_tag="VEL74_11380"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HYG63174.1"
                     /translation="MSRAILPGATVGVLGSGQLGRMFAIAARRMGYRVHTLSPDGDTP
                     TGQVADREVRAAYDDLDAVREFAGRVQVVTFEFENVSAEAAAAAAERAPVRPAGAVLH
                     TTQNRLREKTFLSGAGFPVTPFAPVRSEEDLREGLARLGTPAVLKTAGWGYDGKGQVK
                     IFHPSEAAAAWEGLGRQEAVLEAFVDFQAEVSVVAARGLDGSFAHWGVIENLHRNHIL
                     DVSVAPAAVPQEVEAEAIEIARGILEALDVVGVLCVELFLTGDDRLMVNELAPRPHNS
                     GHLTFDACVTSQFEQQLRAICGLPLGATDLLRPAAMANLLGDLWQKGEPDWAAACRFP
                     GVKLHLYGKAAARPGRKMGHLTAVAGDRETARRLVLDAREALTTKREDRHV"
     gene            complement(1653..2183)
                     /gene="purE"
                     /locus_tag="VEL74_11385"
     CDS             complement(1653..2183)
                     /gene="purE"
                     /locus_tag="VEL74_11385"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HYG63175.1"
                     /translation="MPQETPQENPQESRPGGPPLVAVIMGSKSDWETMRHADEMLTRF
                     GVAHECRIVSAHRTPEWMREFATGAEARGIEVIVAGAGGAAHLPGMVASQTLLPVLGV
                     PVQSHALNGLDSLLSIVQMPSGVPVGTLAIGKAGAANAALLAIRFLAVTRPELRERLR
                     EFVDEQTRRVREETLP"
     gene            2512..>2994
                     /locus_tag="VEL74_11390"
     CDS             2512..>2994
                     /locus_tag="VEL74_11390"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYG63176.1"
                     /translation="MWLALLLLLAVTPWAAAQTDVTTSRISGTVSDAGDSPLPGVTVT
                     ASNTETGLQVSGVTDERGFYRLLNLPTGIYDVAASLDGFVTSQADNVRLLLGSTPTIN
                     FTLSSSSVAETITVTSEAPVVEVTNTTIGTTIQEEQIEALPVAGRDFKNLVLLTPETR
                     L"
BASE COUNT          420 a         1084 c         1092 g          398 t
ORIGIN      
        1 ttcatggtgc cgcggctcag cgtgccgcgg tcgttgtaga aggccagggt gaggtcggtg
       61 gcgtccggca gcgcggcggc caggcgggcg gcgaggcagt cgctcggcac cacgtcgaag
      121 ccgacgccgg gcagcagggc gacgttggcg cgccgcgcct cctccccccg cgacagcacc
      181 tcctcgaaga ccgggatctc gccggtgata tccagataat ggacgccggc ggccaggcaa
      241 gcgtggacca tcggcgcgct ggtgcgcacg aaggggccgg cgcagtggag gacggcggag
      301 aggccggcca agccggcggc cagcgcctgg gggtcatcga gggagaaggc gcgggattcc
      361 agccccagct cgcgggcgat cgcccccacc ttctccggat cgcgcccggc gaggacggga
      421 cgctggccgc ggcgcaccgc ctcccgcgcc gccaactcgc cggtgtagcc cgtcaccccg
      481 tagatcatcc acgccggctc agacatgacg gtcctccctc tttgtggtca acgcctcccg
      541 ggcgtccagc accaggcgcc gggcggtctc ccggtcgccg gccacggcgg tcaggtggcc
      601 catcttgcgg ccgggccgcg ccgccgcctt gccatagagg tggagcttga cacccgggaa
      661 ccggcaggcg gcggcccagt ccggctcccc cttctgccac aggtccccca ggaggttggc
      721 catggccgcc ggccgcagca ggtcggtggc gccgaggggg aggccgcaga tcgcccggag
      781 ctgctgctcg aactggctgg tgacgcaggc gtcgaaggtc aggtggccgg agttgtgggg
      841 gcgcggcgcc agctcgttga ccatcagacg gtcatctccc gtgaggaaga gctcgacgca
      901 gagcacgccg accacgtcca gcgcctcgag gatgccgcgg gcgatctcga tcgcctcggc
      961 ctccacctcc tgcggcaccg ccgccggggc caccgacaca tcgaggatgt ggttgcggtg
     1021 gaggttttcg atgacgcccc agtgggcgaa ggagccgtcg agcccccggg ccgccaccac
     1081 cgacacctcc gcctggaagt cgacgaaggc ctcgagcacc gcctcctgcc gtcccaggcc
     1141 ctcccaggcc gcggcggcct cggacggatg gaagatcttc acctgcccct tgccgtcgta
     1201 gccccagccg gcggtcttga gcaccgccgg ggtgccgagc cgggcgagcc cctcccgcag
     1261 gtcctcctca gagcgaacgg gggcgaaggg ggtgaccggg aagccggcgc cggagaggaa
     1321 cgtcttctcc cgcagccggt tctgggtggt gtggaggacg gcgcccgcgg ggcggaccgg
     1381 cgcccgctcg gcggcggccg ccgcggcctc ggcggagacg ttttcgaact cgaaggtgac
     1441 cacctgcacc cggccggcga actcgcgcac cgcgtcgagg tcgtcgtagg cggcgcggac
     1501 ctcgcggtcg gccacctggc cggtgggggt gtcgccgtcg ggggagaggg tatggacgcg
     1561 gtagcccatg cgccgggcgg cgatggcgaa catgcggccg agctggccgc tgcccagcac
     1621 cccgacggtg gcaccgggca ggatggcgcg gctcacggca gggtctcttc gcgcacccgg
     1681 cgcgtctgct cgtccacgaa ctcgcggagc cgctcccgca actccgggcg ggtgacggcg
     1741 aggaagcgga tcgccagcag ggcggcgttg gcggcccccg ccttgccgat cgccagcgtc
     1801 cccaccggca cgccggaagg catctggacg atggagagga gggagtcgag gccgttgagg
     1861 gcgtggctct ggaccggcac gccgaggacg gggagcagcg tctgcgaggc gaccatcccc
     1921 ggcaggtggg cggcgccgcc ggcgccggcg acgatcactt cgatgccccg cgcctcggcg
     1981 ccggtggcga actcgcgcat ccactccggc gtgcggtggg cggagacgat ccggcactcg
     2041 tgggcaacgc cgaagcgggt cagcatctcg tcggcgtggc gcatggtctc ccagtccgac
     2101 ttgctgccca tgatgacggc gaccaggggc ggcccgccgg ggcggctctc ctgggggttc
     2161 tcctggggtg tctcctgggg catgggtcgg gtccggtccg ggagagggat ggggctgccg
     2221 tccgagctta gcacagggcc gagtcgcggg tccgcgggac cggcgcaggc gactggcagc
     2281 gaggacgggt ggcgggcgga gcgcacacga aggagccgag cagggcccgc ggacccgcga
     2341 ctcggccctg tgcggcacgg ctccgccccc gggccgtcac acagtgctca cacctccccc
     2401 ctcgccccgc ccgccgtcct cgcgtagaat tcaccgaggt ttcctttcgc acttcttctc
     2461 aaggagagaa ctatgttgcg ttcctgtcca gcacatcgca agggagcgtg tgtgtggctc
     2521 gctctgctcc tcctgctcgc ggtgactccg tgggcggcgg cgcagaccga cgtcacgacc
     2581 agccgtatct ccggcacggt gtccgacgcc ggcgactccc ccctgccggg ggtgacggtc
     2641 acggccagca acaccgagac gggcctccag gtgagcggcg tcaccgacga gcgcggcttc
     2701 taccgtcttc tcaacctgcc cacgggcatc tacgacgtcg cggccagcct cgacggcttc
     2761 gtcacctcgc aggccgacaa cgtgcgcctg ctcctcggct cgacccccac catcaacttc
     2821 accctgtcga gctcgtccgt cgccgagacg atcacggtca ccagcgaggc ccccgtggtg
     2881 gaggtcacca acaccacgat cggcaccacc atccaggagg agcagatcga ggcgctgccg
     2941 gtcgccggcc gcgacttcaa gaacctggtg ctgctcaccc ccgagacccg tctc
//
LOCUS       DASPJI010000424         3697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_141421, whole genome shotgun sequence.
ACCESSION   DASPJI010000424 DASPJI010000000
VERSION     DASPJI010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141421"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3697)
                     /locus_tag="VEL74_11395"
     CDS             complement(<1..>3697)
                     /locus_tag="VEL74_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63177.1"
                     /translation="LQPSRDPSRSPLFQTMLILQAVRPGEDPGLAAFSLGEAGARAGL
                     EGLSLESVRLGERRAQFDLTLRLAPVADGGLGAVLEYAADLFEDDTAGRMLGHFPSLL
                     AGAVADPARRLWDLPLLTGAERHQLLVVWNEAGSELPRRDVCLHDLFAEQVQRTPDAE
                     ALVDGRTRLTYGELAARSRRWAGALRDLGVGPEVRVGVCLPRSADLVAVLLGVLEAGG
                     AYVPLDPAYPRERIAFMLEDSGAQVLVTSPEVAPEVPGFKGRRIEMKAGGPHDSPPVP
                     TRPHPAAGPHNLAYLIYTSGSTGRPKGVAIEHRSAAALVEWSRGVFSPEDLAGVLAVT
                     SVCFDLSIFEIFVPLSRGGRVILAENALALPKLPASSEVTLVNTVPSAMAELVRSGKV
                     PRSVRVINLAGEPIPPALAAAIHDLEGPRLYDLYGPSEDTTYSTGTLLVPGGDVTIGR
                     PIAGTRGYVLDHRGGPVPAGVPGELYLGGAGLARGYLGRPDLTAERFVPDALGEVPGG
                     RLYRTGDLVRLRRDGDLDFLGRIDHQVKVRGYRIELGEVEAAIAREPEVRGVAVLALD
                     DGAAADSGRRLAAFVATDLTDLAPVRDSLRRALPEPMVPTRWVALPELPLTPNGKVDR
                     RALARLEVRSGPLEPGGNAPRGGTEERLAALWSEVLGVPRVGRHDDFFDLGGHSLLAT
                     RLAARLHAASGFELPLSAVLRNPTVARLAAFLEQTGGAAGVPKSPPIRPVSREGGLPL
                     SFAQERLWFLDRLVPGSAAYNIPAAIHLSGPLRAAALERALGEVVRRHEALRTVFGGD
                     GGPPVQIVLLPAPPALPVIDLAAGESEAARLAGLEAARPFDLQGGPLLRALLLRLAPE
                     EHVLVLVLHHIVADGWSAGVLLGELAALYDAFAAGRPSPLPELPVQYADYAAWQREHM
                     RGEVLASHVEGWRARLAGLPVLELPADHPRPAVPGFRGAARRTTLPASLAADLRALGQ
                     SQGSTPFMTLLAAFAVLLGRAAGEERLAVGSPVANRGRVEVEGLIGLFVNSLVLPLDL
                     AGDPVFPDLLARVRETCLAAFAHQDLPFERLVEELRPEREPGRNPLFQVLFALEEPLA
                     AREAAGVRFRPEPIENGTAKLDLTFTLTPRAERGLAGRLVVEARYDSDLFEAVTIERL
                     LGQFQILLEGLAADPGARLSALPLLGAAERHQLLREWNDTEAPPPELPVYRLIAAQAA
                     RTPGRTAVALGNRAMTYGELAERAGDLA"
BASE COUNT          555 a         1359 c         1382 g          401 t
ORIGIN      
        1 gccagatcgc ccgcccgctc cgccagctcg ccgtaggtca tcgcccggtt ccccagggcc
       61 accgccgtcc ggccgggggt gcgcgccgcc tgggccgcga tgagccggta gacgggcagc
      121 tccggcggcg gggcctccgt gtcgttccac tcccgcagga gctggtggcg ctcggccgcg
      181 ccgaggagcg gcagggccga aaggcgggct ccgggatcgg ccgccaggcc ttccagcagg
      241 atctggaact ggcccaggag ccgctcgatg gtgaccgcct cgaagaggtc ggagtcgtag
      301 cgcgcctcca ccacgagacg gccggcgagc cccctttcgg cccgaggcgt cagggtgaag
      361 gtgaggtcca gcttggcggt gccgttctcg atcggctcgg gccggaaccg gacaccggcc
      421 gcctcgcgcg cggccagggg ctcctcgagg gcgaagagga cctggaagag cgggttgcgt
      481 ccgggctcgc gctccggccg cagctcctcg accagacgct cgaagggcag gtcctggtgg
      541 gcaaaggcgg cgaggcaggt ctcgcgcacc cgggccagca ggtccgggaa caccggatcg
      601 ccggcgaggt ccagcggcag gaccaggctg ttgacgaaca ggccgatcag cccctccacc
      661 tccacccggc cccggttggc taccggagac cccaccgcca gccgctcctc cccggcggcg
      721 cggccgagga ggaccgcgaa ggcggcgagg agggtcatga acggcgtgct cccctggctc
      781 tggccgagcg cgcgcaggtc cgccgcgaga ctggcgggga gcgtggtccg gcgggccgcg
      841 ccgcgaaagc cggggaccgc ggggcgcggg tggtccgccg gcagctccag caccggcaat
      901 ccggccagac gcgcccgcca gccctccacg tgggatgcca gcacctcccc gcgcatgtgc
      961 tcccgctgcc aggccgcgta gtcggcgtac tgcaccggca gctccggcag cggggaaggc
     1021 cggccggcgg cgaaggcgtc gtacagcgcc gccagctcgc ccagcagcac gccggccgac
     1081 cagccgtccg ccacgatgtg gtggagcacc aggaccagca cgtgctcctc cggagcgaga
     1141 cgcaggagga gcgcccgcaa cagcggccct ccctggagat cgaagggccg cgcggcctcc
     1201 agccccgcca ggcgcgccgc ttcgctctcc cctgccgcga gatcgatcac cggcagggcc
     1261 gggggggcag gaagcaggac gatctggaca gggggcccgc cgtctccgcc gaacaccgtg
     1321 cgcagggctt cgtgccggcg gaccacctcc cccagggcgc gctccagggc ggcggcccgc
     1381 aaggggcccg acaggtggat ggcggccggg atgttgtagg cggcgctccc gggcaccagc
     1441 cggtcgagga accagagacg ctcctgggcg aaggacaggg ggaggccacc ctccctggaa
     1501 accgggcgaa tgggtggtga cttcgggaca cccgcggctc cccccgtctg ctccaggaag
     1561 gcggccaggc gggccaccgt ggggttccgg aggacggcgg acagcggcag ctcgaatccg
     1621 gacgccgcat gcagccgggc ggcgagccgg gtggccagga gcgagtgccc gcccaggtcg
     1681 aagaagtcgt cgtggcggcc gacacgaggg acacccagga cctcggacca gagcgccgcg
     1741 agccgttcct cggtcccgcc gcgcggcgcg ttccctcctg gctcgagcgg cccggaccgg
     1801 acctccagtc gagcgagcgc ccgccggtcc accttgccgt tgggggtcag ggggagctcc
     1861 ggcaacgcca cccagcgggt gggtaccatg ggctcgggaa gcgcccgccg caacgagtca
     1921 cgaactgggg caagatcggt caggtccgtg gccacgaacg ccgccagccg gcggccgctg
     1981 tccgcagcag cgccgtcgtc gagagcaagg accgccaccc cccgcacctc cggctcgcgg
     2041 gcgatggcgg cctcgacctc ccccagctcg atgcgatagc cgcgcacctt cacctggtgg
     2101 tcgatccgcc ccaggaagtc gaggtcaccg tcgcggcgca ggcgcaccag gtctcccgtg
     2161 cggtagaggc ggcctccggg cacttccccc aaggcgtcag gcacgaagcg ttcggcggtg
     2221 aggtccggac ggccgaggta gccgcgggcg agcccggcgc ctcccaggta cagctccccc
     2281 ggaacgccgg ccggcacggg cccgccgcgg tggtcgagga catagccccg ggtccccgcg
     2341 atcggccggc cgatcgtgac atctccaccc gggacgagga gcgtgccggt cgagtaggtg
     2401 gtgtcctccg aggggccgta gaggtcgtag agccgcggcc cctccaggtc atggatcgcc
     2461 gccgcgagag cgggagggat cggctcgccc gccagattga tcacccgcac cgaccgcggc
     2521 accttgccgc tccgcaccag ctcggccatc gccgagggca cggtgttgac caaggtgacc
     2581 tccgacgacg ccggcagctt cggcagggcc agggcgttct ccgccaggat cacccggccg
     2641 ccgcgggaga gcgggacgaa gatctcgaag atggagaggt cgaagcagac cgaggtgacg
     2701 gccaggacgc cggccaggtc ttcgggggag aagacgccgc ggctccactc gaccagcgcg
     2761 gcggcgctcc ggtgctcgat cgccacccct ttggggcggc cggtggagcc ggaggtatag
     2821 ataaggtagg cgagattatg cggaccagcc gcaggatgtg ggcgggttgg gacaggggga
     2881 gaatcgtgag ggcctcccgc tttcatctcg atccgccgcc ccttgaagcc cggcacctcc
     2941 ggcgccacct caggagaggt gaccagcacc tgggcgcccg agtcctccag catgaaggcg
     3001 atgcgctccc ggggataggc ggggtcgagg gggacgtagg cgccgccggc ctccagcacc
     3061 ccgaggagga cggcgacgag atcggcggag cgcggcaggc agaccccgac ccggacctct
     3121 gggcccacac ccaggtcgcg caacgctccg gcccagcggc gggagcgggc agccaactcg
     3181 ccataggtga ggcgcgtcct gccgtccacc agcgcctcgg cgtcgggggt ccgctggacc
     3241 tgctccgcga acaggtcgtg caggcagacg tcccggcgcg gcagctcgct cccggcctcg
     3301 ttccagacca ccaggagctg gtgacgctcg gccccggtga gcagcggcag gtcccagagc
     3361 cgccgcgcgg gatcggccac ggccccggcg aggaggctcg ggaagtggcc cagcatgcgg
     3421 ccggcggtgt cgtcctcgaa aaggtccgcc gcgtattcca gcaccgctcc cagtccgccg
     3481 tccgccaccg gagccagacg cagggtgagg tcgaactgcg cccgccgctc cccgaggcgc
     3541 acggactcca gggacaagcc ttccaggccg gcgcgcgccc ccgcctcccc cagcgagaag
     3601 gcggcgaggc ccgggtcctc ccccggccgc accgcctgga ggatcagcat cgtctggaac
     3661 agcggcgagc gcgacggatc gcgcgacggc tgcaggc
//
LOCUS       DASPJI010000425         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_141461, whole genome shotgun sequence.
ACCESSION   DASPJI010000425 DASPJI010000000
VERSION     DASPJI010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141461"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VEL74_11400"
     CDS             complement(<1..409)
                     /locus_tag="VEL74_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYG63178.1"
                     /translation="MRLSLLLTGGDDAPLLLEVAREADRRGLAALWLPEQHGDGLFPN
                     PAVAAAALAAATRRIGLRAAGLAVPLHNPIRVAEEWSLVDNLSKGRVGIALALADAAA
                     DEGIETVRRLWRGDAARVPDGEGRPIDIRILPRP"
     gene            complement(409..>2755)
                     /locus_tag="VEL74_11405"
     CDS             complement(409..>2755)
                     /locus_tag="VEL74_11405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63179.1"
                     /translation="VYVPLDPQLPTERLTFLVADSGASLLLTEEALLARLPDGPRVVR
                     LDWDGDRDDGSWPETPPFVELHPDALAYVFYTSGSTGAPKGVAVPHGPLAWHEADLAR
                     RFELGPGDRLLGVYAEGFDPSLEQMLATLVSGGAYFPRAGRMWSVPELADRFERWGIT
                     AAMMPSAYWRQAVADLAASGRLLGSGALRVLETGGESMPLELARRWPAVAPGARLLNS
                     YGPTETVITPTLFDVSGEIRSTPSGSVPIGRPVAGRSARVVDRQGRPVPIGVAGELWL
                     GGPLARGYLGRPAATAEAFIPDPDPRAAPGSRLYRTGDRVRLLADGNLEFLGRLDRQV
                     KVRGHRVEPGEVEAALATEPAVREAAVVVQGESLVAWLVPRDPDAAPPDPELLRASLR
                     RRLPEPMIPAAFGWLDALPLTATGKVDRRALAAREIDRPATASQPPRDDLERELARIW
                     EDLLSVRPIGARDDFFALGGHSLLAVRLASRIEAHFGRSVPIATLFERPTVEALADWL
                     RPSFAPVEDSPLVRIQPRGSRPPLFLVHPGGGGVLCYAELARALGPDQPLYGLQAPGL
                     DGERPPLDRIGEMADLYIEALRAVQPAGPWHLGGWSFGGLVAYEMACRLAERGEPAGL
                     VAILDVSPRVAVVSPEEEAELLVRGLDEGFLQDVPVSPAELLGLDTRSQVALVLDRAR
                     RAGRMGDDLDERRAVGLVEVFKANLRAANTWEPRPFPGRITVVRAAGSGHLDAGPDGD
                     WSALAAADMVTVPGDHHSMVTSPHVESLARALRSVLDGAAR"
BASE COUNT          394 a         1013 c         1045 g          303 t
ORIGIN      
        1 ggggccgcgg caggatccgg atgtcgatcg ggcgcccctc tccatcgggg acgcgggcgg
       61 cgtctccccg ccagagccgg cggacggtct cgatcccctc gtccgcggcg gcgtccgcca
      121 gggccagggc gatccccacc cgtcctttag acaggttgtc caccagcgac cactcctcag
      181 cgacccggat cgggttgtga agcgggaccg cgaggccggc ggccctgagg ccgatccgcc
      241 gggtcgcggc cgccagggcc gcggcggcga ccgccgggtt gggaaagagg ccgtcgccgt
      301 gctgttcagg gagccacagc gcggcgagcc cgcggcggtc ggcctcacgg gcgacctcca
      361 ggagaagggg cgcgtcgtcc ccgccggtga ggaggaggct gagccgcatc agcgtgccgc
      421 tccgtccagg accgagcgca gcgcccgcgc cagggattcc acgtgagggg acgtgaccat
      481 gctgtggtga tcgccgggaa cggtcaccat gtcggcggcg gcgagggcgc tccaatctcc
      541 gtccgggccg gcgtcgaggt ggccgctgcc ggcggcccgg acgacggtga tccgcccggg
      601 aaaggggcgc ggctcccagg tattggcggc ccgcaggttg gctttgaaga cctccaccag
      661 gcctaccgcg cggcgctcgt ccaggtcgtc ccccatccgg cccgcccgcc gcgcccggtc
      721 caggaccagg gcgacctggc tccgggtgtc gagccccagc agctcggccg gggagaccgg
      781 cacgtcctgg aggaagccct cgtcgaggcc gcggaccaga agctccgcct cttcctccgg
      841 agacaccacg gcgacacgag gcgacacgtc caggatggcc acgagaccgg ccggctcgcc
      901 tcgctccgcc agccggcagg ccatctcgta ggccaccagc ccgccgaagg accagccgcc
      961 gagatgccag ggcccggcgg gttgaacggc gcgcaacgct tctatataaa ggtcggccat
     1021 ctcgccgatc cggtcgaggg gagggcgctc gccgtcgagc cccggcgcct gcaggccgta
     1081 cagcggctgg tccgggccga gggcgcgggc cagctcagcg tagcagagca ctcccccgcc
     1141 gcccggatgg accaggaaca gcggcggacg gctcccccgc ggctgaatgc ggaccagcgg
     1201 cgagtcttca acgggtgcga aggacgggcg gagccagtcc gccagcgcct cgacggtcgg
     1261 ccgctcgaac agggtggcga tgggtacgga ccggccgaag tgggcctcga tgcgcgaagc
     1321 gaggcggacc gccagcagcg aatggccgcc cagggcgaag aagtcgtcgc gcgccccgat
     1381 cggacgcacg ctgagcaggt cttcccagat gcgcgccagc tcgcgctcca gatcgtcgcg
     1441 cggcggctgg gaggcggtcg cgggccggtc gatctcgcgc gccgcgagcg cccggcggtc
     1501 caccttgccg gtggcggtca gggggagggc gtcgagccag ccgaaggcgg cagggatcat
     1561 gggctcgggc agcctccggc gcagggaggc gcggaggagc tccggatccg gaggcgcggc
     1621 gtccgggtcc cgtggcacca gccaggccac cagactctcc ccctgcacga cgaccgcggc
     1681 ctcccgcacc gccggctccg tggcgagcgc cgcctcgacc tcccccggct cgacgcggtg
     1741 gccccgcacc ttgacctggc ggtccagccg ccccaggaac tcgaggttgc cgtcggccag
     1801 cagtcgtacg cggtcccccg tgcggtagag ccgggatccc ggggcggccc gcgggtccgg
     1861 atcggggatg aaggcttcag cggtggcggc ggggcggccg aggtagccgc gcgccaacgg
     1921 tccgccgagc caaagctcgc cggcgacgcc gatggggacg ggccggccct gccggtccac
     1981 cacccgggcg gagcgccccg ccaccggccg gccgatcggg acggagccgg agggcgtgga
     2041 ccggatctcg ccgctcacgt cgaacagcgt gggggtaatg accgtctccg tcgggccgta
     2101 ggaattgagc agccgggctc ccggcgccac ggccggccat cgccgtgcca gctcgagcgg
     2161 catggactcg cctccggtct ccaggacacg gagcgcgccg gagccgagta gacggccgga
     2221 ggcggccaga tccgcgacgg cctggcgcca gtaggcgctc ggcatcatgg ccgcggtgat
     2281 gccccagcgc tcgaaccggt ccgccagctc gggcacgctc cacatccggc cggcccgggg
     2341 gaagtaggcg ccgccgctca ccagcgtcgc cagcatctgc tccagggagg gatcgaaccc
     2401 ctcggcatag actccgagca gacgatcgcc cggccccagc tcgaaccgcc gggccaggtc
     2461 ggcctcgtgc caggcgaggg gcccgtgcgg gacggccacc cccttcgggg ctccggtcga
     2521 gcccgaggtg tagaacacat aggccagggc gtcgggatgg agctccacaa agggcggggt
     2581 ctccggccag gagccgtcgt cccggtcgcc gtcccagtcg aggcggacaa cccgcggacc
     2641 gtccggcagc cgcgccagga gcgcctcctc ggtcagcagc agcgaggcgc cgctgtcggc
     2701 gaccaggaac gtcaggcgct cggtgggcag ttggggatcg agcggcacgt acacc
//
LOCUS       DASPJI010000426         8931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_770428, whole genome shotgun sequence.
ACCESSION   DASPJI010000426 DASPJI010000000
VERSION     DASPJI010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8931)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8931
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770428"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VEL74_11410"
     CDS             <1..700
                     /locus_tag="VEL74_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYG63180.1"
                     /translation="SLARSNKLPNAEELFSNGPHLATNAFEVGNPALDEETSLGLDLT
                     LRKTTGRLTGELTLFSNRFDDFIFERATGEEEDELPVFQYVQRDAEFRGAELTGVLEV
                     FHAEPYHVDLEFGADFVRAELRDTGEPLPRIPPRRFRLGVHYRGEKLDALIEGQRVSQ
                     QDRVAAFETPTEGHTLLNASVGYRIFTSRAVYDLLLRGTNLTDEEARNHVSFLKDRVP
                     QPGRDIGLALRVGF"
     gene            829..2031
                     /locus_tag="VEL74_11415"
     CDS             829..2031
                     /locus_tag="VEL74_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015118792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HYG63181.1"
                     /translation="MRLVSLLGLVVMLGIAWLLSNNRRRIPWRTVVTGTLLQFAFALL
                     ILKTSGGRAFFGAASEYVTAFLDFSDAGARFVFGDNFTEHFFAFKVLPTIIFFSSFIT
                     VLYYLGVLQWIVKLFAWIMMRLLGTSGAESLSASANIFVGQTEAPLLIRPYVGTMTKS
                     ELMAVMTGGFATIAGGVLAAYVGMGVSAGHLIAASVMSAPAALVMAKIMFPETEESLT
                     AGEVRVEVEKPWANLIDAAAEGATAGLKLVLNVAAMLIAFLALIAVINAILGQLGVWI
                     GFPGLTLEWLLGMVCRPLAWVMGVPWHEADEVGTLLGIKTVLNEFVGYVELEKMAAAG
                     LSERSQAIATYALCGFSNFSSIAIQIGGIGGIAPERKGDLARLGLRAMVAGSLACFQT
                     ATIAGMLL"
     gene            2155..3654
                     /gene="dacB"
                     /locus_tag="VEL74_11420"
     CDS             2155..3654
                     /gene="dacB"
                     /locus_tag="VEL74_11420"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HYG63182.1"
                     /translation="MTGARRFIISSFVVLAVLLGPVSSSVQTASSQGARGAEAQTAQS
                     AAQSALQRTVGFEVNRALQVTAALGVHVMDVDTGETVYAYNPDELRIIASNTKLLTTA
                     AALDALGPGYFFETRFVQRGPVQDGVLRGDLGVIGGGDPHISGRDYDGDSLAVFREWA
                     RALQQRGVRRVTGDLYLDHGLFRPPVVHPDWPPEQLTSWYEAPVDALSFSDNCVLVRV
                     SPASRGGKALVEMVPNVGVFEVDNTSRIVDSRGAHSLVVARKGSTLVVRGGMWKSSGP
                     IETWVTVPDPVTYFGLGVKAALASEGVTLEGGLRPVPRLPGDIWERVAVHRTDLGTAV
                     RVTNKRSQNFYAESLAKLVGATRCSEGSWEEGVRAISEFAVSTGIPEGSFEMADGSGM
                     SRGNRFSPRHLTTLLRHMFFHPASLEFAQSLPYGGEDNGSWKRRLAQSPYRGNVFAKT
                     GTLSGVSALSGYAKAVSGKTYAFSILLNRVGGNAHAAQDRIVMALIDNG"
     gene            3732..5012
                     /locus_tag="VEL74_11425"
     CDS             3732..5012
                     /locus_tag="VEL74_11425"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HYG63183.1"
                     /translation="MITTEARPVNYRTLMKKVEQVVASMGREDDAATSIPSLLDSIID
                     KFRDELGIYGGRLYRRRGGYYVLQGIFGDAKEVPRGLKVPLSYPPIQLLLEQGAVYMD
                     AEDPHIDKSLEDLLGVQQFAAIEVGDEEYILAFNVAPGHHRDDVLFSLGILRHSVNQR
                     LRQERLQGIFEEARKIQASILPRRVPTYGDFDLFGRSEPMEEVGGDFYDYIPLTEKIM
                     GLAIADVSGHGLPAALQVRDIYMGLRMGLARDFKIVRTVERMNRIIHQSTLTSRFVSM
                     FYGELEQNGVFIYVNAGHPPPFHLSADGKVREMTEGGPVLGPLADASYERGFVIMKPG
                     DMMLFYTDGICEAHGREGESGPLEEYGLERLLRVARQSLGKPARDVVDAVFADVEAFT
                     GGADPEDDRTLMVVTYPQVDVNVTQAFPRPVGLI"
     gene            5139..5393
                     /locus_tag="VEL74_11430"
     CDS             5139..5393
                     /locus_tag="VEL74_11430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family RiPP precursor"
                     /protein_id="HYG63184.1"
                     /translation="MSKTELDRLIADLQRHPDLMAELRRLIPDLDRMVRWVAEKGYRV
                     TLAELRQLLDSDRELSDDELEEAAGGEDGWTPPPPPPPAE"
     gene            5525..6361
                     /locus_tag="VEL74_11435"
     CDS             5525..6361
                     /locus_tag="VEL74_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63185.1"
                     /translation="MAPGSAPVDFSLRLKGPEQVKLIAPGLLPPHLEDFLARWAEPGG
                     PFVPVRALWLEFDLDRPVEEAPVPAVIAKLGQGTDPVWALDVLWPALQGSPLHPAQRE
                     RVRACWEAIPPESFLLYAFSLLSRPGGAVRMELFGLDLEGIAGYLGRIAPAAVSRVRE
                     AAALLRDAERLHLSFDVTDEVLPRIGIEGSYPRLPRREPAWVALLDRFVATGLCAPEK
                     RDTLLAWPGYDTFWTAPASWPVGEVGTNGLCVRGLSHLKVVSSPCREPEAKAYLLAGW
                     RA"
     gene            complement(6358..>8931)
                     /locus_tag="VEL74_11440"
     CDS             complement(6358..>8931)
                     /locus_tag="VEL74_11440"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYG63186.1"
                     /translation="QVKGKEEPVLAFKLLGAAPAPSAQVTLSGRALSPFIGRQREMAI
                     LEEVRDQAASGQGQVVGIAAEAGTGKSRLLYELRRRPGTGAGTQSTAWLSGRCLSYGT
                     GTPYLPILYMLRGEWAIGDGDPPAVVTAKVQAGLERLGLDPAAPLPYLLRLLGVEEGT
                     EPLAELSPQALQARTFSVLRQVILASGGGDGLVVLEIEDLHWADQTSEDFLTFLVEGI
                     PAARALLLGTYRPGYRPPWIEKSYATQIPLRRLTDSESRSLVEASLHKLELPEESLSA
                     ILDKAEGNPFFLEELTRSLLERPDVSVPDTVQGVILARIDRLPEEQKRLLQTASVLGR
                     EFSQRLLAAVWERPETLVPLLADLKRWEFLYEEPTAEEPFYFFKHGLTQEAVYQTLLT
                     SRRRALHDSAGEAFETLYSDRLEEVYNRLAYHYSKAEAPARAVRYLALSAEKAARGYA
                     HAEAAQALREALVHAERLPLAERDHSTLELALRLAESLLPLARFAETLEVLSLHQDAL
                     DRVADAALAGRYHFWMAHTHSYMGNQEEVEVHAAHAIDAARQSGDTRIEGRTWYLLCR
                     DAFWGGRFKEGIEDGRRALDLLETSEDRWWQGQALWVAGFHYYVLGRFEEALEAMDQA
                     HAIWEALQDPRLDPSWSTGFFLASMGDWERGIELCRGGLERAGDPLNTAAAQGFLGYA
                     YLEKGDLPRALETLDEAVRSMRQAGMQQLLGWYSAFLAEACLLAGRPAEARERALEAL
                     TVTDGAGFRYGSGFAQRTLGHIAAAEDDPELAEAWLNQALATFRSLDAPFEAARTLLD
                     LVQLAGDQGDLPEAGSRLEEARALFEELRVPAYSARAERLTGSLGLGAQTERRVAPFG
                     V"
BASE COUNT         1275 a         3023 c         3217 g         1416 t
ORIGIN      
        1 gtcgctggcg cggtcgaaca agctccccaa cgccgaggag ctgttctcga acggcccgca
       61 cctcgccacc aacgcgttcg aggtgggcaa tccggccctg gacgaggaga ccagcctcgg
      121 gctcgacctg acgctgcgca agacgacagg ccggctcacc ggcgagctca ccctgttcag
      181 caaccgcttc gacgacttca tcttcgagcg ggcgacgggg gaggaggagg acgagctgcc
      241 ggtcttccag tacgtgcagc gcgacgccga gttccgcggc gccgagctga ccggcgtcct
      301 ggaggtcttc cacgccgagc cgtaccacgt cgacctggaa ttcggcgccg acttcgtgcg
      361 cgccgagctg agggacacgg gcgagcccct gccccgcatc cccccccggc gcttccggct
      421 gggggtccac taccgcggcg agaagctgga cgccctgatc gaggggcagc gcgttagcca
      481 gcaggaccgg gtggcggctt tcgagacccc gaccgaaggg cacacgctgc tcaacgccag
      541 cgtcggctac cggatcttca cctcgcgggc ggtctacgac ctgctgctgc gcggcaccaa
      601 cctgaccgac gaggaggcgc gcaaccacgt ctccttcctc aaggaccggg tgccccagcc
      661 cggccgggac atcggcctgg cgctgcgggt gggcttctag ggagccgggg agagggggcc
      721 gccggtcccc tctccttgcc ttcccccgcc gcgtcgggta gcctcccacg ccaggaaatc
      781 aaccgctcaa cttcccgggg cgcggtcccc ccgcgcgagg aggcaaactt gcggctcgtc
      841 tctcttctgg gcctcgtcgt catgctcggc atcgcctggc tgctgtccaa caaccggcgg
      901 cggatcccct ggcgcaccgt ggtcacgggc accctgctcc agttcgcgtt cgcgctgctg
      961 atcctcaaga cctccggcgg gcgggcgttc ttcggcgcgg cgagcgagta cgtgacggcg
     1021 ttcctggact tctcggacgc gggagcgcgc ttcgttttcg gcgacaactt caccgagcac
     1081 ttcttcgcct tcaaggtgct ccccaccatc atcttcttct cgagcttcat caccgtcctc
     1141 tactacctgg gcgtgctgca gtggatcgtg aagctcttcg cctggatcat gatgcggctc
     1201 ctggggacct cgggcgccga gtcgctctcc gcctcggcca acatcttcgt cggccagacc
     1261 gaggcgccgc tgctgatccg cccctacgtc ggcaccatga ccaagtcgga gctgatggcg
     1321 gtgatgacgg gcggcttcgc caccatcgcc ggtggtgtgc tggcggccta cgtgggcatg
     1381 ggcgtctcgg ccgggcacct catcgcggcg agcgtcatgt cggcgccggc ggcgctggtg
     1441 atggccaaga tcatgttccc ggagaccgag gagagcctga ccgccggcga ggtgcgggtg
     1501 gaggtggaga agccgtgggc caacctcatc gacgccgcgg ccgaaggggc cacggccggc
     1561 ctcaagctgg tcctgaacgt cgccgccatg ctcatcgcct tcctcgccct gatcgcggtg
     1621 atcaacgcca tcctggggca gctcggcgtc tggatcggct tccccggcct gaccctggag
     1681 tggctcctgg ggatggtctg tcggcccctc gcctgggtca tgggggtgcc ctggcacgag
     1741 gcggacgagg tggggacgct cctgggcatc aaaaccgtgc tcaacgagtt cgtcggctac
     1801 gtggagctcg agaagatggc ggcggcgggg ctctcggagc gctcccaggc catcgccacc
     1861 tacgcgctgt gcgggttctc caacttctcc tccatcgcca tccagatcgg cggcatcggc
     1921 ggcatcgcgc ccgagcgcaa gggggacctg gcccggctcg gcctgcgtgc catggtggcc
     1981 ggctccctcg cctgcttcca gacggcgacc atcgcgggga tgctgctgta gcggggcacg
     2041 gttttcgcag agccctccag cggattttct tggctgttct cagcggcggg ggttcatgaa
     2101 tagaagactt tcgatttata gtttcccgtc ggctttttcc gtggaggtcg cagtttgacg
     2161 ggagctcgtc ggttcatcat ctcctcgttc gtggtcttgg cggtgttgct ggggccggtg
     2221 tcttccagtg tccagacggc gtcgtcccag ggggcacgtg gagcggaggc ccagaccgcc
     2281 cagtcggccg cccagtcggc tctccagcgg acggtgggct tcgaggtcaa ccgcgccctc
     2341 caggtgacgg cggcgttggg cgtccacgtg atggacgtcg ataccggcga gaccgtctac
     2401 gcctacaacc cggacgagct gcggatcatc gcctcgaaca ccaagctgct caccaccgcg
     2461 gcggcgctcg acgccctcgg gccggggtac ttcttcgaga cccggttcgt ccagcggggc
     2521 ccggtgcagg acggcgtgct gcgcggcgac ctgggggtga tcggcggcgg ggacccccac
     2581 atctcgggcc gcgactacga cggtgactcg ctggcggtgt tccgtgagtg ggcccgggcc
     2641 ctccagcagc ggggggtgcg ccgggtgacc ggggacctct acctggacca cgggctgttc
     2701 cggccgccgg tggtccatcc ggactggccg ccggagcagc tcacgagctg gtacgaggcg
     2761 ccggtggacg ccctctcctt cagcgacaac tgcgtgctgg tgcgggtgtc gccggcgagc
     2821 cggggtggga aggcgctggt ggagatggtg cccaacgtcg gcgtcttcga ggtggacaac
     2881 acctcgcgca ttgtggactc gcgaggcgcg cacagcctcg tggtggcgcg gaagggctcg
     2941 acgctcgtgg tgcgcggcgg gatgtggaag agctccggcc ccatcgagac ctgggtgacc
     3001 gtccccgatc ccgtgaccta cttcgggctg ggggtcaagg cggccctggc gtcggagggg
     3061 gtgacgctcg agggggggct gcggccggtg ccgcgcctgc ccggggacat ctgggagcgg
     3121 gtggcggtgc accggaccga cctgggcacg gcggttcggg tcaccaacaa gcggagccag
     3181 aacttctacg ccgagagcct ggcgaagctc gtcggggcga cgcgctgcag cgaggggagc
     3241 tgggaggagg gggtgcgggc catctcggag ttcgcggtct ccaccgggat cccggagggc
     3301 tcgttcgaga tggcggacgg ctccgggatg agccgcggca accggttctc gccgcggcac
     3361 ctgacgacgc tcctgcggca catgttcttc catccggcaa gcctcgagtt cgcccagtcg
     3421 ctcccttacg gcggcgagga caacggctcc tggaagcggc gcctggcgca gtccccctac
     3481 cgggggaatg tgttcgccaa gaccgggacg ctgtcgggcg tctcggccct gtcgggctat
     3541 gccaaggcgg tctccggcaa gacgtacgcc ttttcgatcc tgctcaaccg ggtggggggc
     3601 aacgcccacg ccgcccagga ccggatcgtc atggccctca tcgataatgg ttgacgggct
     3661 gacgggctga ccggctccgg ccgtttccgc tatccttccc cggggccatc ccccaagagg
     3721 agaccactgc gttgatcacg accgaggccc gtccggtcaa ctaccggacg ctgatgaaga
     3781 aggtggagca ggtggtggcg tccatggggc gcgaggacga cgccgcgacg tcgatcccct
     3841 ccctgctcga cagcatcatc gacaagttcc gggacgagtt gggcatctac ggcggccggc
     3901 tctaccggcg gcgtggtggc tactacgtgc tccaggggat cttcggggac gccaaggagg
     3961 tgccgcgcgg cctcaaggtc cccctgtcct atccgccgat ccagctcctc ctcgagcagg
     4021 gcgcggtcta catggatgcc gaggacccgc acatcgacaa gagcctggag gatctcttgg
     4081 gagtgcagca gttcgccgcc attgaggtgg gggacgagga gtacatcctg gcgttcaacg
     4141 tggcgcccgg gcaccaccgg gacgacgtgc tcttctcgct ggggatcctg cgtcacagcg
     4201 tcaaccagcg gctgcggcag gagcgtctgc aggggatttt cgaggaggcg cggaagatcc
     4261 aggcctcgat cctgccgcgg cgggtcccca cctacggcga tttcgacctc ttcggccgct
     4321 ccgagccgat ggaagaggtg gggggcgact tctatgacta catcccgttg accgagaaga
     4381 tcatgggcct cgccatcgcc gacgtctcgg gtcacggcct gccggcggcg ctgcaggtgc
     4441 gggacatcta catgggcctg cggatgggcc tcgcccgcga cttcaagatc gtgcgcaccg
     4501 tggagcggat gaaccggatc atccaccaga gcaccctgac cagccgcttc gtctcgatgt
     4561 tctacggcga gctggagcag aacggcgtct tcatctacgt caacgccggc cacccgccgc
     4621 cgttccacct ctcggccgac ggcaaggtgc gggagatgac ggagggcggg ccggtgctgg
     4681 ggccgctggc cgacgccagc tacgagcgcg gcttcgtgat catgaagccg ggcgacatga
     4741 tgctgttcta caccgacggc atctgcgagg cgcacggccg ggaaggggag agcgggccgc
     4801 tggaggagta cggcctggag cgtctcctcc gggtcgcccg gcagagcctg ggcaagccgg
     4861 cccgcgacgt ggtggacgcc gtcttcgccg atgtcgaggc cttcaccggc ggcgccgacc
     4921 ccgaggacga ccgtaccctg atggtggtca cctatcccca ggtggacgtc aacgtcaccc
     4981 aggccttccc gcggccggtg gggctgatct aggcacgccc cgcccgccgt cctcccctcc
     5041 ccggctcggc gccatccttg caggtaacaa cgcaggactt gatttagtcc ccgcgatggc
     5101 gcattgttag cgcatacccc agaagtgagg agacctagat gagcaagaca gagttggacc
     5161 gcctcatcgc ggatctgcag aggcatccgg acttgatggc ggagctccgc cgcctgatcc
     5221 cggacctcga ccggatggtc cgctgggtgg ccgagaaggg ctaccgggtg acgctggccg
     5281 agctgcggca gctcctggac agcgaccgcg agctgtcgga cgacgagctc gaagaggccg
     5341 ctggtggtga ggacggctgg acgccgccac cgccgccgcc ccccgctgag tgacctggga
     5401 cggctggctc tccgtcctac atccgcatct ggcaggcccc ctgtccgatg agcgggctct
     5461 ggatcgtctt ggcgcattga cttgcaggct tccgggcgag tgcctgggag ccttggaggt
     5521 gcgcttggcg cccgggtccg ctccggtgga cttctcgcta cggctgaagg gacccgagca
     5581 ggtcaagctg atcgcgcccg gactccttcc tccccatctc gaagatttcc tcgcgcgctg
     5641 ggcggagccg ggcggcccct tcgtcccggt ccgtgccctc tggctcgagt tcgacctcga
     5701 cagacctgtg gaggaggcgc ccgttcccgc cgtcatcgcc aagctcgggc agggaaccga
     5761 tccggtttgg gccctggacg tgctctggcc ggctctccag gggagccccc tccatccggc
     5821 ccaacgcgag cgggtgcgcg cctgctggga ggccattcct cccgagagct ttcttctcta
     5881 tgccttcagc ctgctctcgc ggccgggtgg ggccgtccgc atggagctct tcgggctaga
     5941 tctggagggc atcgccggct atctgggccg gatcgccccc gccgccgtct cgcgggtacg
     6001 agaggccgcg gccctcctcc gggacgccga gcgcctccac ctctccttcg atgtcacgga
     6061 cgaggtcctg ccgcgcatag gcatcgaagg ttcctatcca aggctgcccc ggcgcgagcc
     6121 cgcctgggtg gcgctgctcg accgcttcgt cgctacgggg ctctgtgcgc ccgagaagcg
     6181 cgacaccctc ctcgcctggc cggggtacga caccttctgg acggcgccgg cgtcctggcc
     6241 ggtgggggag gtggggacca acgggctctg cgtccggggg ttgagccacc tgaaggtggt
     6301 ttcatcgccc tgccgggagc cggaggccaa ggcgtatctg ctggcggggt ggcgggccta
     6361 gaccccgaag ggggcgaccc gtctttcggt ctgggccccc aaccccaagc tgccagtcag
     6421 gcgctccgcc cgcgccgagt acgcggggac gcggagctcc tcgaacaggg cgcgggcctc
     6481 ctcgagacgg ctgccggcct cggggaggtc gccctggtcg ccggcgagtt ggacgaggtc
     6541 gagcagggtg cgggcggcct cgaagggggc gtcgagggaa cggaaggtgg cgagcgcctg
     6601 gttcagccag gcctccgcga gctcgggatc gtcctcggcg gcggcgatgt ggccgagggt
     6661 gcgctgggcg aagccggagc cgtagcggaa gccggcgccg tcggtgacgg tcagcgcttc
     6721 cagcgcccgt tcccgcgcct ccgccggccg gccggcaagg agacaggcct cggcgaggaa
     6781 ggcggagtac caccccagga gctgctgcat cccggcctgc cgcatgctgc ggaccgcctc
     6841 gtccagcgtc tccagggcgc gcgggaggtc ccccttctcc agataggcgt agccgaggaa
     6901 gccctgagcg gctgcggtgt tgaggggatc gccggcgcgt tcgaggccgc cccggcagag
     6961 ctcgatgccg cgctcccagt cccccatgga ggcgaggaag aagccggtgc tccaggaggg
     7021 gtcgagccgc gggtcctgga gagcctccca gatggcgtgg gcttggtcca tggcctccag
     7081 cgcctcctcg aagcggccga gcacgtagta gtggaagccg gccacccaga gcgcctggcc
     7141 ctgccaccag cggtcctcgc tcgtctccag cagatccaga gcgcggcggc cgtcctcgat
     7201 cccctccttg aaccgtcctc cccagaaggc gtcgcggcag aggagatacc aggtgcggcc
     7261 ttcgatccgc gtgtcgccgc tctgccgggc ggcgtcgatg gcgtgagcgg cgtgcacctc
     7321 cacctcctcc tggttcccca tgtagctgtg ggtgtgcgcc atccagaagt gatagcgccc
     7381 cgcgagagcg gcgtcggcca cgcggtccag cgcgtcctgg tgcagagaca ggacttcgag
     7441 ggtctcggcg aagcgggcga gggggaggag ggactcggcg agccgtaggg ccagctcgag
     7501 ggtggagtgg tcacgctcgg ccaggggcag ccgctcggca tggacgagag cctcgcgcaa
     7561 cgcctgcgcc gcctcggcgt gggcatagcc gcgggcggcc ttttcggcgg agagggcgag
     7621 gtagcgcacg gcgcgcgccg gggcctcggc cttggagtag tggtaggcca gccggttgta
     7681 gacctcctcc aggcgatccg agtagagcgt ctcgaaggct tcgcccgccg agtcgtggag
     7741 ggcgcggcgg cggctggtga gaagggtctg gtagaccgcc tcctgggtga gcccgtgctt
     7801 gaagaagtag aagggctcct cggctgtggg ctcctcgtag agaaactccc agcgtttgag
     7861 gtcggcgagc aggggcacca gggtctccgg ccgctcccac accgcggcca gcaggcgctg
     7921 ggagaactcg cggccgagga ccgaggcggt ctggaggagg cgcttctgct cctcgggcag
     7981 ccggtcgatg cgggcgagga tgacgccctg gaccgtgtcg ggcaccgaga catccggccg
     8041 ctccagcagc gaccgggtca gctcctcgag gaagaacggg ttcccctccg ccttgtccag
     8101 gatggcgctc agcgattcct ccggcagctc gagcttgtgc agggaggcct ctaccagcga
     8161 gcggctctcc gaatcggtga gccgccgcag cgggatctgg gtggcatagg atttctcgat
     8221 ccagggcggc cggtagccgg gccggtaggt gccgagcagg agcgcccgcg ccgccgggat
     8281 cccttcgacc aagaaggtga ggaagtcctc ggacgtctgg tcggcccagt ggaggtcctc
     8341 gatctccagg acgaccaggc cgtctccgcc accactggcg aggatcacct ggcgcagcac
     8401 ggagaacgtc cgggcctgga gagcctgggg gctgagctcg gccagcggct cggtgccctc
     8461 ctcgacgccg aggagacgca ggagataagg aaggggggcg gcgggatcga gaccgagccg
     8521 ctcgaggccc gcctggacct tggcggtcac cacggccggc ggatcgccgt cgccgatcgc
     8581 ccactcgccg cgcagcatat agaggatggg gaggtagggg gtgccggtgc cgtaggagag
     8641 gcagcggccg gagagccagg ccgtggactg ggtgccggcg ccggtgcccg gtcgccggcg
     8701 gagctcatag aggagccgcg acttgcccgt gccggcctcg gccgcgatgc ccaccacctg
     8761 cccctgcccg gaggcggcct ggtcgcggac ctcctcgagg atcgccatct cccgctgccg
     8821 tccgatgaag ggggagagcg cccggccgct cagcgtcacc tgggccgacg gcgcgggagc
     8881 ggcgccgagg agcttgaacg ccaggaccgg ctcctccttg cccttcacct g
//
LOCUS       DASPJI010000427         6626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_428523, whole genome shotgun sequence.
ACCESSION   DASPJI010000427 DASPJI010000000
VERSION     DASPJI010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6626)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6626
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428523"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1984)
                     /locus_tag="VEL74_11445"
     CDS             complement(128..1984)
                     /locus_tag="VEL74_11445"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HYG63187.1"
                     /translation="MSPGPLYTNPVYPESFPDPYVLKVRGEYWAYCTGFWPDSRCFGV
                     LRSRDLVHWTPQAGALAPLPGGHPCYWAPEVVEHAGLFYLYYSVGNEERMEIRVAVAD
                     RPGGPFADCGHRLTSEPFAIDAHVFTDPASGVRYLFYATDFLESPQIGTGTVVDRLLD
                     PFTLEGRPRPVALPRFDWHVYHPNRPEKGGVRWHTVEGPFVLERKGLYYEMFSGGNWQ
                     NPTYGVSYATIATLDGPGEWVQAADGEGVLPILRTSGEVVGPGHNSVVRGPDNRQLWC
                     VYHRWSFDGGAPGARVLAIDPLDWAGERLLVLGPTTGPQPAPIPPTLYDESPSQDLYD
                     AGATSFLVEVSARGPGFGLALHGLERPVWRLRVGDDIRLSWREREDLEQTLPLPAGFD
                     VRSFHLLRLDLDGTRVRLAMDGVDLPWTGRLAAPAHGVSLLADGSATELAGFALTVGW
                     EELFVEECDATGLGWQPLHSGSQEKVTGGRLLLASGHSLLVRGPALSSYLLVVNARLD
                     EPADRGWTVHPAAGSGDPGPGLTLERRDGGWALAARDAAGERLFPLPESFDPSMDQQL
                     RCRREGSRVMVEWEGLRLGEVTAPPGPSRIGIGTAGGAVSFEMVRVTALPEI"
     gene            complement(1981..3288)
                     /locus_tag="VEL74_11450"
     CDS             complement(1981..3288)
                     /locus_tag="VEL74_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HYG63188.1"
                     /translation="MKFHPLLPGPRPEEFLWATGIEDTFVPQTRTGHRALDEYQLMGH
                     YEHWREDLDLVRELGVGAMRWGVPWYRVEPFPGEFDWSWTDQVVPYMVDELGITPVID
                     LMHYGCPFWLRREFASYEYPQAVADYAAAFARRYAGRVRWYTPLNEPLVNALFCGKRG
                     LWPPYLRGDAGYIRIMLQLVKGILRTVAALKEVDPGALMIHVEATGLARAARADLEAL
                     AVEDQRRGFLAYDLLTGRLTPEHPLFTWLLRNGARPDELAGLAESAIPLDALGLNFYP
                     QWSTQQVSVDRKGRIAYQAVEQDGAGFTDLIKDYWERYRVPILITETSARGPVEVRER
                     WLEASVAAIRQLREKGIPVLGYTWFPLFTMIDWRYRFGRQPLREYLLDLGLYTLAPED
                     SGRRWDATPLVEDFKHLVRSPAASVGELRALPDRPELQAAAAS"
     gene            complement(3320..4210)
                     /locus_tag="VEL74_11455"
     CDS             complement(3320..4210)
                     /locus_tag="VEL74_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017297678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HYG63189.1"
                     /translation="MRHTPLIPAGPVRDSLAGRLSLKLECFQVTGSFKARGATNKVLA
                     LPAGQAARGLVTASGGSHGLGVAYAGWIAGAPVTIYLPANAPRSKAEKLARWGAKVVW
                     HGEVWDEANLAARQAAERDGLTYVHAFADPAVIAGQGTIGLEILEDAPETGVLLVSIG
                     GGGLIAGIASAVKAVRPDVRVVGVEPVGAPTLRESLRAGRVVELPEIRTVANTLAPRK
                     SHELNLGIIRDTVDEIVLVEDEEMREAAHWLWFEMGVAAELSGAAVLAALRCGRVPEA
                     REVPVCALICGAGTDGLDLC"
     gene            4468..4980
                     /locus_tag="VEL74_11460"
     CDS             4468..4980
                     /locus_tag="VEL74_11460"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG63190.1"
                     /translation="MENTIFNELGHALRWLRDRQGKKQYQVAEAAGITKGMLSAYETG
                     RQKPSLDTLEKILMTLEVNLNDLHAAIQLVNGRPIDPRPRTVSWAGGGGEPAGFGLES
                     GQSVDPRADLYRILAPPDRLPAEEELALTEMLSGIYRLIRYLHRSLRQDFPRASPTPL
                     PFPPVRGDDE"
     gene            complement(5028..6263)
                     /locus_tag="VEL74_11465"
     CDS             complement(5028..6263)
                     /locus_tag="VEL74_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HYG63191.1"
                     /translation="MVVQPIEGGGAVLPPAEPDEFRYARTMPAMSQSTLREVLALASR
                     PGVLSLAVGLPAVELFPAADLARVTSDLLARDPGALNYGIPYQPLKARIVEMMAARGV
                     DCRPEQVFLTTGSQQSMDLLVHMLVDPGAPVLLEETVYDGIQMAMKRLGARPVTVPTD
                     PETGIDVDAIEALLEGGLRPALLYTITDGHNPLGVSISLEKRRRLAELARRHRLPILE
                     DDAYGFLYFGSEPAPPLRAFEDRWVFYLGSFSKTLAPALRAGWAVVPEELVPRLAALK
                     HAADLDTPSFTHRVIAAYLETGRLPEHLALLRGEYRRRRDVILQALDRYFPPGLRWTR
                     PEAGMFVWVELPAGLDTAQILRRAVEDEQVAFSPGVAFAAGGSHANHCLRLNFTFCQP
                     EQIEEGVRRLARVLTAVLG"
     gene            complement(6281..>6626)
                     /locus_tag="VEL74_11470"
     CDS             complement(6281..>6626)
                     /locus_tag="VEL74_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HYG63192.1"
                     /translation="SEEAVRALRPDFGRLRTLPVRGVIATARASTAPFDFVSRFFAPG
                     AGVDEDPVTGSAHCTLGPYWAGRLGKEDFLAYQASERGGVLQVRVAGERVELAGQAVT
                     VLRGHLLDEIAH"
BASE COUNT         1040 a         2336 c         2354 g          896 t
ORIGIN      
        1 ccttgctgac gacaggagcg agcggcgggg cggcaccggc gcccagccga ggagggttct
       61 ctggtcagac aacttggcaa ttcccgcaac gggactcctc cccgcccgcc tcgtcgccag
      121 ttttctttca gatctccggc aacgctgtca ccctgaccat ctcgaaggac accgcgcccc
      181 cggcggtccc gatcccgatc cgggacgggc ccggtggggc cgtgacctct cccagacgca
      241 gcccctccca ctcgaccatc acccggcttc cctccctgcg gcagcggagc tgctgatcca
      301 tcgagggatc gaagctttcg gggagaggga agagccgctc cccggcggcg tcccgcgccg
      361 ccagcgccca gcctccgtcg cgccgctcca gggtcagccc agggcccggg tcgccggagc
      421 cggcggcggg atggacggtc cagcctcggt ccgccggctc gtcgaggcgg gcgttgacca
      481 ccaggagata gctggaaaga gctgggcccc tgaccagcag ggagtggccc gaggcgagca
      541 ggaggcgccc tcctgtgacc ttttcttgcg atccggaatg taaaggctgc caaccgaggc
      601 cggtggcatc acattcttcc acgaacagct cctcccagcc cacggtcagg gcgaacccgg
      661 ccagctccgt ggcgctcccg tcggcgagaa gggagacacc gtgagccggc gcggcgagcc
      721 gtccggtcca cggcaggtcg acgccatcca tggccaggcg gacgcgggtg ccgtccagat
      781 ccaggcggag cagatggaag gaccggacgt cgaaccccgc cggcaggggc agcgtctgct
      841 cgagatcctc cctctcacgc caggacagcc ggatgtcgtc gcctacacgc aaccgccaaa
      901 ccggcctttc cagaccgtgg agcgcgaggc cgaagcccgg tccgcgggcg ctcacctcga
      961 cgaggaagga ggtggctccg gcgtcgtaga ggtcctggga ggggctctca tcgtagagag
     1021 tcggagggat gggcgcgggt tgcgggccgg tggtgggtcc gaggaccagc aggcgctccc
     1081 ccgcccagtc cagcggatcg atggccagca cccgggcgcc gggggcaccg ccgtcgaagc
     1141 tccatcggtg atagacgcac cagagctggc ggttgtccgg gccgcggact acggagttgt
     1201 gtcccggccc caccacctcg ccggaggtcc gcaggatggg cagcaccccc tcgccgtcgg
     1261 cggcctggac ccactccccg gggccgtcga gggtggcgat ggtggcgtag ctcaccccgt
     1321 aggtgggatt ctgccagttg ccgccgctga acatctcgta gtagaggccc ttgcgttcga
     1381 ggacgaacgg cccctcgacg gtgtgccagc gcacgccccc cttctccggg cggttcgggt
     1441 ggtagacgtg ccagtcgaag cgcggcagcg ccaccggccg cggccgtccc tccagggtga
     1501 aggggtcgag gagccggtcg accacggtgc cggtcccgat ctgggggctc tccaggaagt
     1561 cggtggcata gaagaggtag cggacgcccg aagccggatc ggtgaagaca tgggcgtcga
     1621 tggcgaacgg ctcggaggtg agacggtgcc cgcagtcggc gaacgggccg cccgggcgat
     1681 cggcgacagc gacccggatc tccatcctct cctcgttgcc cacgctgtaa tagagataga
     1741 agaggccggc gtgctcgacc acctcgggcg cccaatagca gggatggccc cccggcagcg
     1801 gcgccagggc tccggcctga ggggtccagt ggaccaggtc gcgggagcgc agcaccccga
     1861 agcagcggct gtcgggccag aaaccggtgc aataggccca gtactccccc cggaccttga
     1921 ggacgtaggg gtccgggaac gactccgggt agaccgggtt cgtgtacagc gggcccgggc
     1981 tcacgacgcg gcggccgctt gcagctcggg acggtccggc aacgcgcgca gctcccccac
     2041 cgacgccgcg gggctccgga ccagatgctt gaagtcctcg acgaggggtg tcgcgtccca
     2101 ccggcggccg gagtcctcgg gcgccagggt gtagaggccc aggtccagca gatactccct
     2161 caggggttgc cggccgaagc ggtaccgcca gtcgatcatg gtgaacaggg ggaaccaggt
     2221 gtagccgagg acggggatcc ccttctcccg gagctggcgg atggcggcca cggacgcctc
     2281 gagccagcgc tcgcgcacct ccaccgggcc ccgggcgctg gtctcggtga tgaggatggg
     2341 gacgcggtag cgctcccagt agtccttgat gaggtccgtg aagccggcgc cgtcctgctc
     2401 cacggcctga taggcgatcc ggcccttgcg gtccaccgac acctgctgcg tggaccactg
     2461 gggatagaag ttgaggccca gggcatccag ggggatagcg ctctcggcga ggcccgccag
     2521 ctcgtccggg cgggcaccgt tgcgcagcag ccacgtaaac agcgggtgct ccggggtgag
     2581 ccggccggtg aggaggtcgt aggccaggaa gccccgacgc tggtcctcga ccgccagggc
     2641 ctccaggtcg gcccgggccg cgcgggcgag gccggtcgcc tccacgtgga tcatgagcgc
     2701 tccggggtcc acctccttca gggccgccac cgtgcggaga atccccttca cgagctggag
     2761 catgatccgg atgtagccgg cgtcgccccg caggtagggg ggccagaggc cccgcttgcc
     2821 gcagaagagg gcgttgacca gcggctcgtt gagcggcgtg taccagcgta cccggcccgc
     2881 gtagcgccgg gcgaaggcgg cggcgtagtc cgccacggcc tgcgggtact cgtagctggc
     2941 gaactctctg cgcagccaga aggggcagcc gtagtgcatc aagtcgatga ccggcgtgat
     3001 gcccagctcg tccaccatgt aggggaccac ctggtcggtc cagctccagt cgaattcgcc
     3061 ggggaacggc tcgactcggt accacggtac cccccaccgc atggcgccga cgcccagctc
     3121 ccggacgagg tccaggtcct cgcgccagtg ctcgtagtgg cccatgagct ggtactcgtc
     3181 gagagcccgg tgacccgtgc gggtctgggg gacgaacgtg tcttcgatgc cggtcgccca
     3241 gaggaactct tcgggccggg gacccggcag cagaggatgg aacttcaact acgtgctcct
     3301 tcggatgaag gggcagacct tagcacagat cgaggccgtc ggtgccggcg ccgcagatca
     3361 gggcgcagac agggacctcg cgggcctccg gaaccctccc gcaacggagg gccgccagca
     3421 cggcggcgcc cgagagctcg gcggccaccc ccatctcgaa ccagagccag tgcgcggcct
     3481 cgcgcatctc ctcgtcctcc accaggacga tctcgtccac cgtgtcgcgg atgatgccca
     3541 ggttgagctc gtggctcttc cgcggcgcca aggtgttggc gaccgtccgg atctccggca
     3601 gctccaccac ccggccggct cgcaggctct cccggagggt gggcgcgccc accggctcga
     3661 cgccgaccac ccggacgtcc ggacgcactg ccttcaccgc ggaggcgatc cccgcgatga
     3721 gaccgccgcc gccgatggac accagcagca cgccggtctc gggagcgtcc tcgaggatct
     3781 ccaggccgat cgtcccctgc cccgcgatca ccgccgggtc ggcgaaggca tggacatagg
     3841 tcagcccgtc ccgctcggcc gcctgtcgcg ccgccaggtt cgcctcgtcc cagacctccc
     3901 cgtgccagac caccttcgct ccccagcgcg ccagcttctc cgctttggac cgcggcgcgt
     3961 tggccggcag gtagatggtc acgggggcgc ccgcgatcca accggcgtag gccaccccga
     4021 ggccgtggct gccgcccgag gcggtgacca ggccgcgggc cgcctgcccg gcgggcagcg
     4081 ccagcacctt gttcgtcgct ccccgggcct tgaacgagcc ggtgacctgg aagcactcga
     4141 gcttgaggga gagccggccg gccaggctgt cgcgcacggg acccgccggg atcagcggcg
     4201 tgtgccgcac gtgggctcgg atacgccgct ccgcggcacg aatgtcttcg aggagcaccc
     4261 tggaagaccc cgcttcgacg ggagaagaag attcttcggg ttggaccatt gacttacgct
     4321 atcattgcgc ctaagctacc cacatgcaga aacgagccgg aacactgacg gtcgattcgt
     4381 ggggcgagac gggcgcgcac gtcctctccg ggaaccggcg gcggtggcag acccggtgct
     4441 cgtcaaagga ttcaggagac tcataccgtg gagaacacaa tcttcaacga gctcgggcac
     4501 gccctgcgct ggctccggga ccgtcaagga aagaaacaat accaggtggc cgaggccgcc
     4561 ggaatcacca agggcatgct gtcggcctac gagaccggcc ggcagaagcc gtcgctcgat
     4621 acgctggaga agatcctcat gacgctggag gtcaatctga acgacctcca cgccgcgatc
     4681 cagcttgtca acggccggcc gatcgacccg cggccgcgga cggtgtcctg ggcgggcgga
     4741 ggcggcgagc cggccggctt cggcctcgaa agcgggcaga gtgtggaccc tcgcgccgac
     4801 ctctaccgca tcctggcgcc gccggacagg ctgccggccg aggaggagct ggcgctgacg
     4861 gagatgctct cgggcatcta ccggctgatc cgctacctcc accggtcgct gcggcaggac
     4921 ttcccgcgcg ccagccctac ccctctccct ttcccgcccg tcaggggcga cgacgagtga
     4981 ggcacggcac cggcgcgtcc ggtgcccttc ctctcgatcc gcgcggctca gcccaggacc
     5041 gcagtcagta cccgggcgag ccgccggacc ccctcttcga tctgctcggg ctggcagaag
     5101 gtgaagttga gcctcaggca gtggttggcg tggctgcctc ccgccgcgaa cgccacgccc
     5161 gggctgaacg ccacctgctc gtcctccacc gcccggcgga ggatctgcgc ggtgtccagg
     5221 ccggccggca gctccaccca gacgaacatg ccggcctccg gccgcgtcca gcggagcccc
     5281 ggagggaagt accggtcgag ggcctggagg atcacgtccc ggcggcgccg gtactcgccc
     5341 cgcagcaggg cgaggtgctc ggggagcctg ccggtctcca gataggccgc gatgacgcgg
     5401 tgagtgaagg aaggcgtgtc gaggtccgcc gcatgcttga gtgccgcgag ccgggggacc
     5461 agctcctcgg ggaccacggc ccagcccgcc cgtagcgccg gcgccagggt cttggagaag
     5521 gaccccaggt agaagaccca gcgatcctcg aaggcacgca gcggcggcgc cggctcgctg
     5581 ccgaagtaga ggaagccgta ggcgtcgtcc tccaggatcg gcaggcggtg gcggcgggcg
     5641 agctctgcca gccggcgccg cttctccagg ctgatgctaa cccccagcgg gttgtgcccg
     5701 tcggtgatcg tatagagcag agccgggcgg aggccgccct ccagcagcgc ctcgatggcg
     5761 tccacgtcga tgccggtctc tggatcggtc gggacggtga ccggcctcgc ccccaggcgc
     5821 ttcatggcca tctggatacc gtcgtagacc gtctcctcga ggagcaccgg cgcgccggga
     5881 tcgacgagca tgtggaccag gaggtccatg gactgctgcg agccggtggt caggaacacc
     5941 tgctccggcc ggcagtcgac gccgcgggcg gccatcatct ccacgatgcg cgccttgagc
     6001 ggctggtacg ggatgccgta gttgagcgcg cccgggtccc gcgccagcag gtcggaggtc
     6061 acccgcgcca ggtcggcggc ggggaacagc tcgacggcgg gcagaccgac cgccagcgac
     6121 aggacgcccg ggcgcgaggc cagcgccagc acctcgcgca gggtggactg gctcatcgcc
     6181 ggcatggtgc gggcgtaccg gaactcatcc ggttcggcgg ggggcagaac ggcgcctcct
     6241 ccttcgatgg gttgaacgac catggtgacc tccacctccc tcagtgcgcg atctcgtcca
     6301 acaggtgacc ccgcagcacg gtcacggcct gcccggcgag ctcgacgcgc tcccccgcta
     6361 cccgtacctg gaggacgccg ccgcgctcgg aggcctgata cgccaggaag tcctccttcc
     6421 ccaggcggcc ggcccagtag ggaccgaggg tgcagtgggc ggagccggtc accgggtcct
     6481 cgtcgactcc agcgccgggg gcgaagaacc gggagacgaa gtcgaagggc gcggtcgagg
     6541 ctcgggcggt ggcgatcacg ccgcggaccg gcagcgtccg cagccgcccg aagtcgggac
     6601 gcagggcgcg gacggcctcc tcggag
//
LOCUS       DASPJI010000428         5422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_141511, whole genome shotgun sequence.
ACCESSION   DASPJI010000428 DASPJI010000000
VERSION     DASPJI010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141511"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1642)
                     /locus_tag="VEL74_11475"
     CDS             complement(158..1642)
                     /locus_tag="VEL74_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HYG63193.1"
                     /translation="MGIRSRIRGLACGLVLLAAAWPAAGQGNRAALYEEGEQAQRLIV
                     RLTAANTEGAGILFHADAQYAYGITAKHVVFQQGKAVQGLQALLYAWPGRQLPVAAEG
                     LRFHYQEDLAVFRVDLRPLGKSLQEVLQGIPLEQLGSSEELDPGSGLFTIGHATAGAW
                     ISSKSTVPFVRDEGSAFLFNTLCSQGHSGGGVFDDKWRLVGMMIKDESPLCRALRIEP
                     ILRIVQGWKLEIGLRPPAGDKPDKGVSKGIRVAIVDFDNRSGRDMPKLGFMAQDHTTT
                     ALYTVPGVTLVSRDRLESIQREHQIPGSIQTSAEEASRVGKLLKADVLVTGSILRYDV
                     ERRTFEGFGTSALQDTSLMAIGFQILDVESGEVRFSKTFDVERTKQYPKATSAPSQPI
                     DLTSDLLAALLELARNDLRSALSQVAAGKGRAGQFVQVPVTSKPAGADVIVDGTYLGS
                     TPMTLQLTTNSEKEIAIELAGYESWRRRVKVQPGITIDVSLLRR"
     gene            complement(1645..2436)
                     /locus_tag="VEL74_11480"
     CDS             complement(1645..2436)
                     /locus_tag="VEL74_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63194.1"
                     /translation="MTRCVIDRLIVAVFFSLLSGLAGAESFCDPNLVDRSDSSLAYRE
                     REGRCEGIYGQQVSAVSIDVRSLVAVFAPFDPARDTELVLAWTAPPGTELNVRVRAFS
                     LQPRKYFRMDTAVPASRGAYRWPTDVLSRLGLGQEALGVIAWMALPGPGGTTREVYLP
                     LRAGSGAAAPEEGYQVALVPSKRLREVHVSVSRLDDRGGVAADLRRGEELGYGYYPSN
                     EPTVFSTGRLGSAGFYLVEIAATPVEGTPVKQGFELYHSGDSKGD"
     gene            complement(2454..3308)
                     /locus_tag="VEL74_11485"
     CDS             complement(2454..3308)
                     /locus_tag="VEL74_11485"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HYG63195.1"
                     /translation="MFLSRLYLLIPLMLCLAVPAPAEGEKSIYKLGLILGDEKQNKGH
                     NDEAINAVTDAFVDSRRFTLVERKQLNAVLTEKNLQEFIGGQVNNKLSDLLGLDLVGV
                     VAYNVEKIQSPEGKLVPNWILEVRLIDVKSAQVLSTLTSERASLLSSTTPREAGRRLF
                     HSIREAFPPMGYVVRVDGKEILINLGSEAGVKDGDTLEIVQQGEQIPDPVTGQLLSAP
                     MKVIGELKVLSTSPQISICKFKSKKGEVGPTTLVRLQGKSSLIIEWLSKVPRLKQELK
                     RKNSEIRE"
     gene            complement(3220..5382)
                     /locus_tag="VEL74_11490"
     CDS             complement(3220..5382)
                     /locus_tag="VEL74_11490"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYG63196.1"
                     /translation="MPEMTYLESVLDLSSLRSARFSWGGRDYRGATGLDEERAVDLCA
                     LELEPESYGRELYDAVFPPGSELREGLREAVLAAEREKSRLRFLLHLSPELPEWVHAL
                     YWELLADQSRHLALARSPDTAFSRYLSVRRQPGAPTTRRPRLLYAVAAPADLARFGLA
                     AIDRDVIVQRLAEPFSVLADAVEITLLEPPVTLERLRQRLTTDGGFHLLHFFGHGDRR
                     SGVSSLVLEDEQGRAKFVGEEILAELFLGLRELRLVTLVACHGGAPSSHDPFSGLAGR
                     LVQRGVPAVIAMRRAVSVETGHLFTDHLYRQIAHTGRADAAVNEARQQLYLADPQGIA
                     WSSPVLYSRLVDGRLWVPQTEPPPAGESVAGRPLVRFAPRRLLRPLPWVPALAALALF
                     GLGRWPAAEAEAAFDLRVSQLSFRLAKAAVVLESLELAELAATRLAALRLPSLLPESP
                     PPSPGPAGGMTGILVAAGAGARLTLNAQPLPAGTEVVFEHQDGALYRLSLAGSGRELE
                     ATFQGDVLVKPLHAAPARLRLEQPESLALSPQGGVAELDLTFSRFTPDALSPDIPIDR
                     LSLRRIGEQHTLGTTLVREESTVLGGTVSVRRTGRIHTLEKSEWLRYAALDGSLAGLR
                     LAEDGVRLEFQGRVSGLERAAEGFEPESLMPSVLDLWITGPLQAAVQRGAAVLAVLIL
                     FFMLIERRTNVPITAVSADPSDAVPRGAGAGGGREEYL"
BASE COUNT          881 a         1856 c         1847 g          838 t
ORIGIN      
        1 cgggccgcgg cacttccatc tgccgttggc gctcgctatc ctcacggtgg ccgaaagccg
       61 catgccggag atcctggccg tggagtgagg cggccggacg ccactggagc tgccgattgg
      121 cgagcaccag tgaggcccac agccgtagag ctcagtgcta cctgcgaagc aggctgacat
      181 caatggttat cccgggctgg accttgacgc gacggcgcca ggactcgtac ccggccagct
      241 cgatggcgat ttccttctcg ctgttggtgg tgagctggag cgtcatcggt gtgctgccca
      301 gataggtgcc gtccacgatg acgtccgccc cggccggttt cgaggtgacc ggcacctgga
      361 cgaactggcc cgcccgcccc ttgcccgccg ccacctgcga cagggcgctc cgcagatcgt
      421 tcctcgccag ctcgagcagt gccgccagca ggtcgctcgt cagatcgatc ggctgcgagg
      481 gggccgaagt cgccttcggg tactgcttcg tgcgctcgac gtcgaaggtc ttcgaaaagc
      541 gcacctctcc actctcgacg tcgaggatct ggaagccgat ggccatcaac gaggtgtcct
      601 gcagcgcgct ggtgccgaag ccctcgaagg tgcggcgctc gacgtcgtag cgcaggatcg
      661 agccggtgac caggacatcg gccttgagca gcttccccac ccggctggcc tcctcggccg
      721 acgtctggat cgagcccggg atctgatgct cgcgctggat ggactccagc cggtcgcgag
      781 acaccagcgt cacccccggt acggtgtaca gggcggtggt ggtgtggtcc tgggccatga
      841 agcccagctt cggcatgtcc ctgccgctgc ggttgtcgaa gtccacgatc gccacccgga
      901 tccccttgga tacccccttg tccggcttgt cgcccgccgg cggacgtagg ccgatctcca
      961 gcttccagcc ctgaacgatg cgcaggatcg gctcgatgcg gagggcgcgg cagagggggc
     1021 tctcgtcctt gatcatcatg cccaccaggc gccacttgtc gtcgaacaca ccgccgcccg
     1081 agtgcccctg cgagcagagg gtgttgaaca ggaacgcgga gccctcgtcg cggacgaacg
     1141 gcacggtgct cttggagctg atccaggcgc ctgccgtggc gtgcccgatc gtgaacagtc
     1201 cgctgcccgg gtccagttcc tcggaggagc ccaactgctc cagcgggatt ccctggagca
     1261 cctcctggag cgacttgccc agcgggcgca gatccacgcg gaacaccgcg aggtcttcct
     1321 gataatgaaa gcgcagcccc tcggcggcga ccgggagctg gcggccgggc caggcgtaga
     1381 ggagcgcctg gagcccctgg acggccttgc cctgctggaa gacgacatgc ttggcggtga
     1441 tgccgtaggc gtactgcgcg tcggcatgga agaggatacc ggccccctcg gtgttggccg
     1501 cggtgaggcg cacgatcagc ctctgcgcct gctcgccctc ttcatagagg gcagcgcggt
     1561 tgccctggcc cgccgccggc cacgccgccg cgagcagaac aagaccgcag gcaagccctc
     1621 gaatcctgga tctgatcccc acgttcaatc tcctttcgaa tcgccagagt gatagagctc
     1681 gaagccctgc ttgaccgggg taccctcgac gggcgtggcc gcgatctcca cgagatagaa
     1741 gcccgccgag ccgagccggc cggtggaaaa gaccgtcggc tcgttcgacg ggtagtagcc
     1801 gtagcccagc tcctccccgc gacgcagatc cgccgccact ccgccgcggt cgtccagccg
     1861 gctcacgctg acgtggacct cccgcaggcg cttcgatggg acgagggcga cctggtatcc
     1921 ctcctccggt gcggcggcac ccgatcccgc gcggagcggg agatagacct cccgggtcgt
     1981 gccgccgggg ccgggcagcg ccatccaggc gatgaccccg agggcctcct ggccgagccc
     2041 caaccggctc aggacgtcgg tgggccagcg atacgcgccg cgggaggccg gcaccgccgt
     2101 gtccatgcgg aagtacttcc tcggctgcag gctgaacgcc cgcacacgga cgttcagctc
     2161 cgtgccgggc ggcgccgtcc acgccagcac cagctccgtg tcccgcgccg gatcgaaagg
     2221 ggcgaagacg gcgacgagcg agcgcacgtc gatgctcacc gcgctcacct gctggccgta
     2281 gatcccttca caacggcctt cgcgctcgcg gtaggccagg gagctgtcgg accggtccac
     2341 caggttcgga tcacagaagc tctccgctcc cgccagcccg gacaggagag agaagaagac
     2401 ggcgacgatg agtcgatcga tcacacaacg ggtcatggct ctctcctcct cggtcattcg
     2461 cggatctcgc tgttcttcct cttcagctcc tgcttgagcc gaggcacctt cgacagccac
     2521 tcgatgatga gagagctctt cccctggagt cgtaccaggg tggtggggcc gacttctccc
     2581 ttcttcgact tgaacttgca gatggaaatc tgaggcgagg tggagaggac cttgagctca
     2641 ccgatgacct tcatgggagc agagagcagc tgtcctgtca ccgggtcggg gatctgctcg
     2701 ccctgctgca cgatttccag ggtgtcgcca tccttgaccc ccgcctcgct ccccagattg
     2761 atcaggatct ccttgccatc gacccggacc acatagccca tgggagggaa cgcttctcgg
     2821 atgctatgga agagccgccg ccccgcttcc ctgggagtcg tggaggaaag aaggctcgct
     2881 cgctcgctgg tcaacgtcga caggacctga gccgatttca catcgatgag ccgtacctcg
     2941 aggatccagt tcggaacgag ctttccctcg ggtgactgga tcttctcgac gttgtaggcg
     3001 acgaccccca cgaggtcgag gccgaggagg tcggagagct tgttgttgac ttggccgccg
     3061 atgaactcct gcaggttctt ctccgtcagg acggcattga gctgcttgcg ctccaccagg
     3121 gtgaagcgcc tggaatcgac gaaggcgtcg gtgactgcgt tgatcgcttc gtcgttgtgg
     3181 cctttgttct gcttctcatc gccgaggatg aggcccagct tatagatact cttctcgccc
     3241 tccgccggcg ccggcaccgc gaggcacagc atcagaggga tcagcagata cagccgtgat
     3301 aggaacattc gttctccttt cgattagcat gaagaacagg atcagcaccg ccaggacagc
     3361 agcccccctc tgcacggcgg cctggagcgg tccggtgatc catagatcga gtacggaggg
     3421 catgaggctc tcgggctcga agccttccgc cgcgcgctcc agcccggaga cgcggccctg
     3481 gaattcgaga cggacgccgt cctcggccag acgcagtccg gccagcgagc cgtcgagcgc
     3541 ggcgtaccgc aaccactcac ttttctcgag cgtgtggatc cggccggtgc gccgcacgct
     3601 caccgtgccc cccaacaccg tcgactcttc ccgcaccagg gtcgtgccga gggtgtgctg
     3661 ctcgccgata cggcgcaggg acaggcgatc gatggggata tcgggagaga gggcgtccgg
     3721 cgtgaaccgc gagaaggtga ggtcgagctc cgcgacgcct ccctgcggcg agagggcgag
     3781 cgactcgggc tgctccaggc ggagccgggc cggcgcggcg tggagcggct tcaccaggac
     3841 atcgccctgg aacgttgctt cgagctcccg gcccgagcct gccagggaga gccggtacag
     3901 cgcgccgtcc tgatgctcga agaccacctc cgtacccgcc ggcaagggtt gggcgttgag
     3961 ggtgagccgc gccccggcgc ccgcggcgac cagaatccct gtcatcccac ccgcgggacc
     4021 ggggctcggc ggcggcgatt ctggtaggag ggacggcagg cgcagggcag cgagccgggt
     4081 ggccgccagc tccgccagct ccaggctctc gaggaccacg gcggctttcg ccagccggaa
     4141 ggagagctgc gagacccgga gatcgaaggc ggcttcggcc tccgcggccg gccagcggcc
     4201 gagcccgaag agggcgaggg cggcgagggc cggcacccag gggagcggac gcagcagtcg
     4261 gcgtggagcg aaacggacga gcgggcgccc ggcaacgctc tcccccgccg gcggcggctc
     4321 ggtctgcggc acccagagtc tcccgtccac cagacgcgag tacagcaccg ggctgctcca
     4381 cgcgatcccc tgaggatccg ccaggtagag ctgctgccgc gcttcgttga ccgcggcgtc
     4441 ggcgcggccg gtatgagcga tctgacggta gaggtgatcg gtgaagagat ggccggtctc
     4501 gacgctcacc gcgcggcgca tggcgatcac cgccgggacg ccgcgctgga ccaggcggcc
     4561 agccaggccg ctgaacgggt cgtgggacga cggtgccccg ccgtggcagg cgaccagagt
     4621 caccagacgc agctcgcgca ggccgaggaa gagctccgcg agaatctctt cgccgacgaa
     4681 cttcgccctc ccctgctcgt cctcgagcac gagggaggag acgccgctgc ggcggtcgcc
     4741 atggccgaag aagtggagga gatggaagcc gccgtccgtc gtcagccgct gccgcagccg
     4801 ttccagcgtc accggcggct cgaggagcgt gatctcgacc gcatcggcca acacgctgaa
     4861 cggctcggcg aggcgctgca cgatgacgtc gcggtcgatc gcggccaggc cgaagcgcgc
     4921 gaggtcggcg ggcgcggcga ccgcgtagag caggcggggc cgccgcgtgg tgggcgctcc
     4981 cggctgccgg cgcacgctca gatagcggga gaaggcggtg tcgggggacc gggccagggc
     5041 caagtggcgg ctctgatcgg cgagcagctc ccaatagagg gcgtggaccc actcgggcag
     5101 ctcgggagag agatgcagca ggaaccgcag gcggctcttc tcccgttcgg cggcgaggac
     5161 cgcctcccgc aggccctccc gcagctcact ccccggcggg aagaccgcgt cgtacagttc
     5221 ccggccgtag ctctcgggct ccagctccag ggcgcagaga tccaccgctc gctcctcgtc
     5281 gagccccgtt gccccccggt agtcccgtcc gccccaggag aagcgagccg agcggaggct
     5341 ggacaggtcc agcacgctct cgagataggt catctccggc atggctccgg ctcccccgct
     5401 ttctggaagc acggcgtcag gc
//
LOCUS       DASPJI010000429        20828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_711181, whole genome shotgun sequence.
ACCESSION   DASPJI010000429 DASPJI010000000
VERSION     DASPJI010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 20828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20828)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20828
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711181"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VEL74_11495"
     CDS             <1..974
                     /locus_tag="VEL74_11495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYG63197.1"
                     /translation="EGELRLAGVQTAVAERRSLTPTSRAVGVVTADESRVRRVQVRIT
                     GWVEKLHVNTTGQPVRAGQALLSIYSPELLATQEEFLRAREAAARFSDSSLPEVRRGG
                     QDLLEAARRRLELFDVPRGVISRLESTGKAQRAITLTSPASGYVTGKEVFEGMQVGPG
                     MDLLTVTDLSRVWVEADFYQFESRSVGVGQRATISLPDDPGTRLAGRAAFVYPALDPQ
                     SRTLKVRYEFANPGLVLKPGMYVDVMPELGTSEGVVVPDSAIIDTGTRQVVFVAKDGT
                     FEPRQVRVGTRAEGEAMILSGLSEGESVAVRANFLLDSESRLRAAIQ"
     gene            971..4192
                     /locus_tag="VEL74_11500"
                     /pseudo
     CDS             971..4192
                     /locus_tag="VEL74_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002734646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
     gene            complement(4977..5879)
                     /locus_tag="VEL74_11505"
     CDS             complement(4977..5879)
                     /locus_tag="VEL74_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63198.1"
                     /translation="MVWDSEQYWRKAIQYAEMASEPEREAWERPFWLSLALEFLARAS
                     ITKVHPALNADPEGDGLNLLYAFGYELKGQPKSLPIHAVFLRLQKILPDTFTKPRREF
                     CDYFSNLRNQELHTSELPFESLSETKWLARCYDVCEVLCNHLGKTLIDLFGEEAGSAG
                     ALIRALKAEKTSAVKSKIAAHEKVFDEKPEEEREQLVLQQATLSEGWFGTATRIECPA
                     CESWARLLGTVERVSKPIYDDGALFVKNVVLANRLECKACGLVLADIDELHIAEIGPH
                     FECEEVTELHEYHEADFGPEYDNM"
     gene            complement(5863..6552)
                     /locus_tag="VEL74_11510"
     CDS             complement(5863..6552)
                     /locus_tag="VEL74_11510"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HYG63199.1"
                     /translation="MFSTLLLPENWKRLVCDGYCVLNNVDAEALLPIARELGHPHFDP
                     RDRVLVKDIRPQPVAVANANTLSSRYGMGAFPLHTEAAYLPRPPRFLLLYCVRPGSGG
                     RTTILLDAAKVFSRISCRRRLGTWVVKAGRRPFLCHALWGSPPKDFGIRYDRECLFPR
                     GPAAQAEDQLISDFISRSTPTKIVWTEKKLLVVDNLRMLHGRGEANDDDRDRWLKRVL
                     VAEEALNGLGF"
     gene            7081..7638
                     /locus_tag="VEL74_11515"
     CDS             7081..7638
                     /locus_tag="VEL74_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63200.1"
                     /translation="MRSFTNNSTPSVRSNAFTTTFLDRLAERDEPPTAGDADVAGPWR
                     IESIPSHGFGLFRVGESLARGFAPVAVFPSRWLALLMAGLLPGTGRDPLLLLSKQPDA
                     HGAYPVTLAGGGDDGGDGGDDDGNGRRPIGFLQLFDERAIDALNVVIHLLQSPESLAY
                     VLEAAGSVTLERCGAILDERAAAPE"
     gene            complement(8932..10206)
                     /locus_tag="VEL74_11520"
     CDS             complement(8932..10206)
                     /locus_tag="VEL74_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HYG63201.1"
                     /translation="MKRSCFCLLLAVMTISFAAHAQDQPPNPAVVHLMEAYSVDQAEA
                     QKRLDLQFKVAELSDRLNKENDPAYADMYITHKPRYRVIVRFAGNSNKSRKALVDSLD
                     PQLRPHVKLRGAKRSRGATARSLDQLNAALRQLSIPFTSEYNLETQRFSVIVEDPSDV
                     ERVESVLPRARRRDTTVEVGLIPKIEALPTGVQPGDRLYGGNPIFTSPGGAGGYCSLG
                     YAVSFTLNGVAKRGILTAGHCPDTMYVDFGDHSVTLSGPVINKPHRDAPPDGADGISD
                     KYDYQIWDTTGLTVDNTIAYEDRNGIPEFPAAGTFRMTSIMTFLNQKAGMIVCKSGDA
                     TGITCGEIVNGNATRDGVAGWIRVSNTQQADISEGGDSGGPWFLYPGTSSTITGVGIH
                     TAGSGTGSTSTAVYMPIDYIDDHIGSVNTIKQ"
     gene            complement(10208..10546)
                     /locus_tag="VEL74_11525"
     CDS             complement(10208..10546)
                     /locus_tag="VEL74_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63202.1"
                     /translation="MPAGEQPTSMPFIAETRSPVEVISLASPGRLEVIDGCLTVAIQG
                     SERATAVFPPGVKPELRGNDVVAVSFEGRRFLIGEETLIPGGGIRLSSAELVKPIPSY
                     CPKVLFGLGG"
     gene            complement(11134..11544)
                     /locus_tag="VEL74_11530"
     CDS             complement(11134..11544)
                     /locus_tag="VEL74_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYG63203.1"
                     /translation="MKITIETVVKAELNQVWDAWNNPEDIKKWNSAQDDWHTTQSSVD
                     LREGGKFSSRMEARDGSMGFDFEGTYTRVEPHKAIEYRMSDGREVQIEFVEQADGVLV
                     KETFDAETENSPEMQREGWQAILDNFGRYVEAKG"
     gene            11671..12294
                     /locus_tag="VEL74_11535"
     CDS             11671..12294
                     /locus_tag="VEL74_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYG63204.1"
                     /translation="MSPDTTTWNRWRYTVWAPIYDAVARFGRQRRRSLALLALQPGER
                     VLVVGAGTGADLPFIPAGVEVLATDLTPAMLARARSHARPGVELQVMDGQALDLPDAS
                     FDAVILHLIVAVIPDPVACLREAARVLKPGGRMAVFDKFLPDGARPSPPRRAANALTS
                     FLFTDINRRFGDILAASGAPLRVEHDEPALFGGMFRILRLVKLQSVQ"
     gene            12325..12753
                     /gene="mscL"
                     /locus_tag="VEL74_11540"
     CDS             12325..12753
                     /gene="mscL"
                     /locus_tag="VEL74_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HYG63205.1"
                     /translation="MGMIDEFKEFAVKGNAIDLAVGVVLGAAFGKIVTSLVDDIIMPP
                     IGLALGGVDFTDLFVNLGPGFYASLEEAKAAGAPTLNYGNFLQNIVDFLLVALALFLI
                     VRVINRLRRRREEAPAAPAAPPEEVALLREIRDSLQRRPV"
     gene            complement(12775..13101)
                     /locus_tag="VEL74_11545"
     CDS             complement(12775..13101)
                     /locus_tag="VEL74_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003880942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYG63206.1"
                     /translation="MAFYTQLGFKLEQRPGPAFASVTNGNLSLWLSGPQSSGSRPLPD
                     GRRQEPGGWNRLVLEVDDLPSRVAAMKAAGFRFRNEIETGPGGKQIQLEDPDGNPVEL
                     FEPARP"
     gene            complement(13362..14183)
                     /locus_tag="VEL74_11550"
     CDS             complement(13362..14183)
                     /locus_tag="VEL74_11550"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYG63207.1"
                     /translation="MHYRSHTPAAPLSSFIERFWLCSDAPPHARERILPSGTIELVIN
                     LREDEIRTYDSSQPDRCTRYSGAVVSGTYSRFFVIDPFQHASILGVHFKPGGAFPFLG
                     LPADELADAHVDLGWLWGAQAAELRERLCAAATAAARFSLLEQALLSRLIHSPARHGA
                     VPAALGAFGQTGSEVRVREVAERVGLSHRRLIQVFAAEIGMTPKLYCRVRRFQRARAL
                     AQTAAPDWASIAVDCGYFDQSHLIRDFLAFSGLSPADYLRRRTGQVLPNHIPLSG"
     gene            complement(14191..15516)
                     /locus_tag="VEL74_11555"
     CDS             complement(14191..15516)
                     /locus_tag="VEL74_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HYG63208.1"
                     /translation="MPNTPTRRLAILSFLLVFAGSGSILRSASATVVPGSDPRRPVVI
                     TVDDLPIVSAKLHPDPAERERITREMLLALRKHGIRAVGLVTWDRVQGEKDVELLRMW
                     LEQGHELGNHSYKHLSYTRTAPAEYIADVEAGRKQVAALLEPSGRTVRFFRFPFLREG
                     DTPEKLDAMRAYLAQSGQRNLPVTLDNQDWSFEEPWVEARKKGDRQAMDRVAEQYHES
                     LHLEIRGHEAVGDRLFGRQTPQILLLHATEVSAAQWDRLFTWLTETGHRFAGSDEVLG
                     DPAFSEPHRYVGHFGPGLWDRIAAERRTKTDVAEVEALLKKQAEAWSRGDLEAFTSVY
                     ADDATFLTSSGVTQGRQAVLDRYRKRYPDRQAMGTLTLEVLETRPASGTEVTMLGGAR
                     PSRVHGVSVAARWKLSYPEGGERKDAEGLTLIVLRRGANGWEIVQDASM"
     gene            complement(15527..16330)
                     /gene="truA"
                     /locus_tag="VEL74_11560"
     CDS             complement(15527..16330)
                     /gene="truA"
                     /locus_tag="VEL74_11560"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HYG63209.1"
                     /translation="MPTYRLTLEYEGTRYRGWQEQQNAKSVAGELRRAVEETGGAVQE
                     IGGAGRTDAGVHALAQAAHLRLRRPIDPEPFRRELNDLLPPDIHVLSLTPAPDRFHAR
                     HDAISRSYVYQICRRRTALAKRFVWWVKRPLDVRRMSEAAALLPGRHDFRLFCEKPAE
                     QTSTLVVVEGLEVKTDGALILVRVTASHFLWKMVRRVVGTLVQVGGGDLDLAGFEKLL
                     AGQAPRSGKGLPAEWTAPPSGLFLERVLYKGDPPLGPLVPAVPVAGEGF"
     gene            complement(16337..18670)
                     /locus_tag="VEL74_11565"
     CDS             complement(16337..18670)
                     /locus_tag="VEL74_11565"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HYG63210.1"
                     /translation="MVDRMETSNTVEPGETPRWGLAAALVLLALVLALVVMRSQAPAP
                     RPADAPAAEFSAGRARQVQQELLGDQRPHPVGSPANALVRDRIIARLRALGYEPDVQR
                     AFSCNLGGVGNCAWVENVFARLPGRQSTGVVMMMAHYDSVAGGPGASDAMSGVAAILE
                     VARILKAGPPLRNDVLLVLDDGEEAGLLGAKAFADYHPAAREVKAIINLEARGSAGPS
                     LMFETSGPNAWLVSSYARNAPRPVTSSVFATIYDQLPNDTDLTVFKRRPDPVPGLNFA
                     YIRKPAHYHTALDNLETASPASLQHHGDNALAAVRGLGEADIANPPRGSAVFFDVLSL
                     FVVRWPEPWTLGLAALALILVVAAVARSVRSRTLTGRAVVAGVLGFLLVLIAGALAAF
                     LLQFALRALGAMNVAWLANPLPYLAALWLLVLGVVLLAAGLFRRAGLGGLWAGVWLWW
                     SVLGLVVAFLATGISYLFVVPALVAGLAGALLPRGRWALAAILPLVVAALLWFPVILP
                     LYDGLGGGAFLVIGVLLTLLLTALAPLVPGSGALWRRWVPLGAVVLAVVLLMVGRQSP
                     VFTETSPQPVSLQYHLDGDTGQARWLARFAPPFPESLRQGAKFGDSAVQPFPWSPPFA
                     RAFAAPAPAAALPAPDLTVLENTATGGKRTVRFRLSSPRGAWIAGLIMPAAAQVESME
                     VGGIPLRLDELTGRGGPQPGSREAPAYRTLTFLSVPPQGIEVQAVLGASAPADWYVLD
                     RSPGLPAGGEALVKARPATASTFQDGDTTMVSRKVRL"
     gene            18815..20206
                     /locus_tag="VEL74_11570"
     CDS             18815..20206
                     /locus_tag="VEL74_11570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HYG63211.1"
                     /translation="MNLSQEELLFGRIALHYKLASQEQLNAAAAILTSEGGLRGLGEI
                     LVDQGILQQIQLDQLRAVQRDYVAKKQGQTATAASAPMTIEAADAAPLPESPMPTEAP
                     EPAPPPEPSWRTAERPLLELLVQAIRQGASDIHVHCGATPKVRLQGTLEEVDSTLLTA
                     AETDSWVRDVLTPEQIEALEKHGQVDFAYNIPGVGRFRSNAYRQQRGVDAVFRAIPPT
                     PPTLEGLGLPGSLARFTDFHQGMVLITGPSGCGKSSTLAALINILNESRPDHVITIED
                     PIEVLHPSKRCVVNQRQVGRHTGSFARALRAALREDPDIIAIGELRDLETIQLALTAA
                     ETGHLVLATLHTSNAIRTVNRILGVFPPAQQEQIRTMFSESLRAVVSQRLVNRADGAG
                     RVPALEVLVANKAVGNLIRDNKTFQLRSVIQTGGSQGMCLLDASLQELVKNKVITRQE
                     ALRHCEEPQRFAG"
     gene            20221..>20828
                     /locus_tag="VEL74_11575"
     CDS             20221..>20828
                     /locus_tag="VEL74_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYG63212.1"
                     /translation="MARLHELFLHLKNHGASDLHLASGLEPRVRVHGELEAVPGWPVL
                     DNEAMLGLLREIASDEQWEEYRRCGDLDFAYGFEGVARFRANYLEQSNGAGAVFRMIP
                     EEIVPLEKLKLPKAIESLAHLQQGLVLVTGPTGSGKSTTLAALIDYINTTRAAHIMTV
                     EDPIEYLHRDHHSIVNQREVAVDTRTFSHALRSALRQDPDVIL"
BASE COUNT         3467 a         7143 c         6805 g         3413 t
ORIGIN      
        1 aggagggcga gctgcgcctg gccggcgtgc agaccgccgt ggccgagcgc cgctccctga
       61 cccccaccag ccgcgccgtg ggagtggtca ccgccgacga gagccgggtc cgccgggtac
      121 aggtgcggat caccggctgg gtcgagaagc tccatgtcaa caccaccggt cagccggtgc
      181 gtgcagggca ggcactgctg tcgatctact cgccggagct gctcgccacc caggaggagt
      241 tcctgcgcgc ccgcgaggcc gcggcgcgct tctcggattc gtcgctgccg gaggtgcggc
      301 gcggcggcca ggacctgctg gaggcggccc gccgccggct cgagctgttc gacgtgccgc
      361 gcggcgtcat ctcccgcctg gagagcaccg ggaaggccca gcgcgccatc accctcacct
      421 cccccgcgtc gggctacgtc accggcaaag aggtcttcga ggggatgcag gtggggcccg
      481 gcatggacct cctcaccgtc accgacctct cccgcgtctg ggtggaggcc gacttctacc
      541 agttcgagag ccgctccgtg ggcgtgggcc agagggcgac catctcgctg cccgacgatc
      601 ccggcacccg gctcgccggc cgcgccgcct tcgtctaccc ggccctcgac ccgcagagcc
      661 ggaccctcaa ggtgcgctac gaattcgcca accccggcct ggtgctcaag cccgggatgt
      721 acgtggacgt gatgccggag ctcggcacca gcgagggcgt ggtggtgccc gactcggcca
      781 tcatcgacac cggcacgcgg caggtggtct tcgtggccaa ggacggcacc ttcgagccgc
      841 gccaggtgcg ggtaggcacc cgcgccgaag gcgaggcgat gatcctctcc ggcctctccg
      901 aaggcgagag cgtggccgtg cgcgccaact tcctgctcga ttcggagtcg cgtctcaggg
      961 cggcgatcca atgatcaaaa agatcatcgc cttctcggcc cacaaccgct ggctcgtcct
     1021 gctggccgtg gccgcgctct gcgtgctggc gggctggatg ctccaggaga tccggctgga
     1081 cgccctgcca gacctctcgg acgcccaggt catcatctac tcgcgctggg accgctcccc
     1141 cgacatcctc gaggaccagg tcacctatcc catcgtcacc gctctcctcg gcgcgcccgg
     1201 ggtcaaggcg gtgcgcggct tctccgactt cggtttctcg tacgtctacg tcatcttcca
     1261 ggacggcacc gacctctact gggcgcgctc gcgcgtgctc gaataccttt cgaagatcca
     1321 gccccgcctg cccgaggggg tgcagaccga gctcggcccg gacgccaccg gcctcggctg
     1381 ggtcttccag tacgccctgg tcgaccgctc gggcaagcac tcgctggacg agctgcgctc
     1441 ctaccaggat tggaccctgc gctacgccat ccagtcggtg cccggcgtcg ccgaggtggc
     1501 ctcaatcggc ggcttcgtca agcagtacca gatcaccgtc gatcccaacc ggctggccgc
     1561 ctacggcatg ccgctcgacg aggtggtgga ggcggtgcgc agctccaacg acgagatggg
     1621 cggccggctg atcgaaggca gcggcaccga gatcatggtg cgcgcccgcg gctatgcccg
     1681 gagcgtccag gatttcgagc agatcgtgct ggacgtgggc cctgccggca accccgtgct
     1741 gctgcgcgac gtcgcccggg tggagctcgg cccggagatc cgccgcggcg tggccgacct
     1801 cgacggtctg ggggatcacg tgggcggcat cgtggtcatg cgccacggcg agaacgcgct
     1861 caacgtgatc catcgcgtca aggagcggct gcacgagctc cagcccggga tgcccgaagg
     1921 ggtcgaggtg gtcaccacct acgaccgctc ggacctcatc acccgggcgc tggacacgct
     1981 gaaacacgag ctggtgctgg agatgatcgt cgtctccctg atcatcctgc tcttcctctg
     2041 gcacatcccg tcggcggcgg tgccgatcct caccatcccg atctcggtgc tgctcgcgtt
     2101 cattccgctg tacctgttcg ggatcacggt gaacatcatg tcgctcgccg gcatcgccat
     2161 ctccatcggc gtgctggtgg acggcgccat cgtcgaggtg gagaacgcct acaacaagat
     2221 ccaacgctgg caggcggcgg gcaagccggg ggatttccat cagatccgcc tggaggcgct
     2281 ccaggaggtg gggccgtcgg tcttcttctc gctgctggtg atcgcggtcg cgttcctgcc
     2341 ggtcttcgcc ctggtggacc aggaggggcg gctcttcaag cccctagcct actcgaaaaa
     2401 cctgacgatg ttcatcgccg cgctgctggc tatcacgctg gacccggcga tgcgcatgct
     2461 cttctcgcgc atggaaccgt tcacgttccg gccgcgctgg ctcgcccgtc tggccaccgg
     2521 cgcgaccgtg ggcacctact accccgagga gcggcacccg atcagccggg cgattcacaa
     2581 ggtttatgat ccggcctgcc gcttcgtgct gcggcgaccg ttcctcacca tcggcgtggc
     2641 cttcgcactg gtggccgtga gcatcccggt ctacttcaag ctaggctcgg agttcatgcc
     2701 gccgctgaac gaggggagca tcctttacat gcccacgacg ctccccggcg tgtcggtggc
     2761 gcaggcgcag gagctgctcc aggcgcagga ccgggtgctc aaggccttcc ccgaggtgga
     2821 gcgggtcttc ggcaaggcgg gccgcgccga gacctcgacc gacccggcgc cgttctccat
     2881 gttcgagacc accatcattc tcaagcccga ggaccagtgg cggccgaagg agcgctggta
     2941 ctcggactgg ccggagtgga caaaacccgc cttccgtccc ttctggcccg accgcatcac
     3001 ctgggacgag ctggtggccg agatggaccg cgcggtcaag gtgccgggca tcaccaacgc
     3061 ctggaccatg ccgatcaagg cgcgcaccga catgctctcc accggcatcc gcacgccgat
     3121 cggcatcaag gtctttggcg aggatctgaa cgtgatcgaa cgcctcggcg ccgagatcga
     3181 ggggatgctc caggacgtcg agggcacgcg cagcgtctac gccgagcggg tggccggcgg
     3241 ctacttcctg gacatcgtgc cgcgccgcga tcagctcgcc cgctacggcc tcaccgtcgg
     3301 ccagatgcag atggtgatcg ccagcgccgt aggcggcgag aacgtgacca ccaccatcga
     3361 gggacgggag cgttatccag tcaacgtgcg ctatccgcgc gagctgcggg acagcccgga
     3421 gcgcctgggc cgcgtgttga tcaccacctc gtcggggcag caggtgccgc tcgcccaggt
     3481 ggcggacatc caactcgtca ccggcccgtc catggtgcgc gacgagaacg gcttcctcgc
     3541 cggctacgtt tatgtggaca tcaccggccg cgacgtcggc ggttatgtgg aagatgccaa
     3601 gaaggtggtg cgcaaccacc tgaccctgcc cgccggctac tccctgcagt ggagcggaca
     3661 gtacgagaac atgctgcgcg tgcgggagcg gctgaagatc gtggtgccga tcaccttggc
     3721 actgatcttc atcctgctct acgcgaacac gaagtcggcg ttcaagtcca tggtggtgat
     3781 gctggccgtg ccgttctcgg ccatcggcgc catctggctc ttctatctcc tggattacaa
     3841 cgtctccatc gccgcctggg tggggatgat cgcgctgctc ggcctggacg ccgagaccgc
     3901 ggtcttcatg ctgctcttcc tcgacctcgc ctacgacgag gccaagcggg aggggcggct
     3961 gcgcacggtg gcggagttgg atgaggcgat cctccacggc gcggtgaagc gggcgcggcc
     4021 gaagatgatg accgtggccg ccgccttcat gggcctgctg ccgatcatgt ggtccacctc
     4081 ggccggctcc gacgtgatga agcgcatcgc ggcgccgatg atcggcggcc ttgtcacttc
     4141 gttcctgctg gagctgctgg tctatccggc catctacaaa atctggaaac agcgcacgga
     4201 cctgcgtgcg accgatatga atgtgaaagg agccaacgtt atgaagaagc agatcccgac
     4261 cgtcacggcc ctcctcctgg tgctgggcct cgccactctc tcccccgccg tcgcctctgc
     4321 cgcgacaggt ttcgaatccc tggccggccc ctacgaagcc gtccgccagg ccctcctcca
     4381 cgacaagatg accggcgtgg ccgagcaggc gaagctgctc cagcagcggt cccaggccct
     4441 ctccaagggc tttaccccgc aggccgccgg cgtggccgcg gacaaggctc cgatggtcaa
     4501 gaccctcctc cccgaggtga ccgccgccgc ggagcgcctc gccgccgcga aggacctgac
     4561 ggcggcccgc gccgcctttg gtgatctctc gaagccgatg gtgcgctacc gcgaggtgtc
     4621 caacggcaag cggcccgtgg tcgcctactg cccaatggcc aagaagtcct ggctgcagcc
     4681 ggccggtgag attggcaacc cgtactacgg ccagtcgatg gccaagtgcg gggatgtggt
     4741 gtcgaagtag acgaagtcgt caggaactcg gatgaccggc cgcagagagg cggcaacctc
     4801 caggctgcct cacagaccag tcatccgagt tttctagtcc ctgccctgcc gagtacaagc
     4861 ctttttctat aaacgcctcc agttaggccc tcagttacaa ttgcagctct ggccaaaaag
     4921 caggcaactg ctgccacaat tggtttaaca tcgctcgcta gccaccacag gactcgctac
     4981 atgttgtcat actcaggacc gaagtccgcc tcatgatatt cgtgcagctc tgttacctct
     5041 tcgcattcga aatgaggccc gatctcggcg atatggagtt catcaatgtc cgcgaggacc
     5101 agaccgcatg ccttgcactc caggcggtta gccaggacca cgttctttac aaacagcgcg
     5161 ccgtcgtcat agattggctt ggagacgcgc tcaacagttc ccaggaggcg ggcccatgat
     5221 tcgcacgccg gacactcaat cctggtggcg gtcccgaacc aaccttccga gagagttgcc
     5281 tgttggagca cgagctgctc cctctcttct tctggtttct catcgaagac tttttcatgt
     5341 gccgcgatct tcgacttcac cgccgaagtc ttttcggctt tcagcgccct gatcagcgcc
     5401 ccggcgctac cagcctcttc cccgaaaaga tcgatgagag tcttgcctag atggttgcac
     5461 aagacttcgc agacgtcata gcagcgcgcg agccactttg tttcggacag gctctcgaac
     5521 ggcaattcgg acgtgtgcag ctcttggttt cggaggtttg aaaaatagtc acaaaactct
     5581 cgccggggct tagtgaatgt gtcgggcaag attttctgta gccgcaggaa gaccgcgtga
     5641 atcggaagcg atttcggctg gcccttcagc tcgtagccga acgcatacaa aaggttcagg
     5701 ccgtcgccct ccggatcggc gtttagggcg ggatggacct tcgtgatgga tgcccgggca
     5761 agaaattcca atgccagaga aagccagaag ggcctctccc acgcctctcg ttcaggctcg
     5821 gaagccatct ctgcatactg gattgctttc cgccagtatt gctcagaatc ccagaccatt
     5881 aagagcttcc tccgcgacga gaacacgctt tagccagcgg tctcgatcgt catcattggc
     5941 ctcccctcgg ccatgcagca tccgtaagtt atcgaccacc agaagcttct tctcggtcca
     6001 aacgatcttc gttggtgtcg atcttgaaat aaaatccgaa atcaactggt cctccgcctg
     6061 ggccgctggc ccccttggga atagacattc acgatcatac cggatgccaa aatccttggg
     6121 tggacttccc cacagcgcgt gacagaggaa tggacgccgc ccagctttta caacccatgt
     6181 gcccagccgt cggcggcaag aaatgcgact gaaaactttc gccgcgtcaa gcaggatagt
     6241 agtgcgtcca ccactgccgg gccttacgca gtacagcaac agaaatcggg gaggcctcgg
     6301 caggtaggca gcctcagtgt ggagagggaa ggctcccata ccataccgcg atgacagtgt
     6361 attggcgttg gcgaccgcca cgggctgggg acggatgtct ttgaccagaa cgcggtctct
     6421 tggatcaaaa tgcggatgcc caagttcccg cgcgatggga agcaaagcct ccgcgtcaac
     6481 gttattaagg acgcaatagc cgtcgcagac tagtcgcttc cagttttcag gtaacagcag
     6541 cgtggaaaac acatctagct cgctgaaggc tgtcagtcag ctaagcctaa tccgtctggc
     6601 tctcggcgtc aaatggatcg gatgttggcc ataggaggac tctggcgcta cctatcgggg
     6661 tggtgacgcc cagcggtgag ccgcaggtcg ggcgagccgt gtcagcgtct cacctgccgg
     6721 gggttctcgt tgtccctgaa gggctgctgg gatgcaagaa gccggatggg gcttgtggtg
     6781 catccggccg gcccccttat agccgctcat cgatggatcg agcacataga atctcacaaa
     6841 atcttcgctc accctcttga ctcccctctc caccctcgat agactccaag cataaacccg
     6901 gcgttcgcag tcttcccggg gaggagggtg gtcggccaga agaggaagaa agacaggcag
     6961 ttccccgacg acatctctgg aaggggggac ggctggcatg aggccagctt acggccagga
     7021 agagagctgg taactctctc cccaggcctg accccgcaaa cgtcctttct tggaggacct
     7081 atgcgaagct tcaccaacaa tagcactccg tccgttcgtt caaacgcctt caccaccacc
     7141 ttcctcgacc ggctggccga gcgggacgag ccgcccacgg ctggcgatgc cgacgtcgcc
     7201 gggccttggc ggatcgagtc catcccgagt cacggcttcg gcctgttccg ggtgggcgag
     7261 agcctcgctc gcggcttcgc cccggtggcg gtcttcccct cccgctggtt ggcgctcctc
     7321 atggcgggcc tcctccccgg caccggccgg gacccgctcc tcctcctctc caagcagccc
     7381 gacgcccacg gcgcctatcc cgtcaccctc gccggtggcg gtgatgatgg cggcgacggc
     7441 ggggacgacg acggcaacgg ccgccgtccg atcggcttcc ttcagctctt cgacgaacgg
     7501 gcgatcgacg cactcaacgt cgtgatccac ctgctccagt cgccggagtc tctggcctat
     7561 gtgctggagg cggcgggttc ggtgaccctc gagcggtgcg gggccatcct cgacgagcgg
     7621 gcggcggcgc cggagtagga cgtccttatc tatagggggc gccgccttgg tggcggcgcc
     7681 cccgtttctc ctcggccgtc ccggcacggg atgggccgcc gccccccgca cctggggttg
     7741 acggcccagg ccgctggcac gctcactccc atggtgcggc cctcatcccc cgagaaacga
     7801 aggccgtacc tcgggtacga gagccatccc tggagggatg accgtcctcc ctccggggac
     7861 gtgctgatcc ccgcgggcag gaccaccatc tccgcgggta ggatgaccct accccgggta
     7921 cggtcatcat cccccgcggt agggtgacca tctccttggg tatggacctc atcccccgat
     7981 cgccaatgcc tcggcgaccg cctagcctcg ggaggcggag gacggttccc tccgcctccc
     8041 acttcggggc ggcagttctg aggggacggc cttggtcacg gcggccccct ctagattcag
     8101 ttgtcaaaga gcaacctgtc gccgcgcccc agacaggacg aatgaagaca ggagcgcgca
     8161 tcataccctg ctcctccgtc actattgaca aaacaggcat cggcatgcca ctctccggct
     8221 ccagaaggga ataagacgcc cacccttggg gaggggtccg aatggcagga aggttctttg
     8281 acaactgatg caccaagtcc cgggcgcggc gagggcggcg ccgtgcccgc ggatcgcctg
     8341 cctttccgtc ctggcgttcc cgtttttccg gcccaggccg ctggcacgct cactcccagg
     8401 gtgcggcggt catccctcga gaaacgaagc ccgtacctcg gggacgccta cccgcggaga
     8461 tggtggtcct acccaccgga ttcgcacgtc ccccgaggga ggacgggcac gctccctctc
     8521 ccgcaagcgc tgatgcgaag ctcgggagat acgaatcgtg gctccaggca gctcaaatcc
     8581 ccctctggag agaagaatca gaatccgcct cggctgattc gaattcaact ctgttcgact
     8641 cggcgccggc ggcctccgat tcgaatccag cggcctacga atcaaccgga gcggaatacg
     8701 ctacgccact cggaggggac gagaccgctg aggcaggagt cggatcagcg cctccaggat
     8761 ttggatcaag tctcgccgga gtcgaatcga atcgccctcc ttccgatgcg aacgacggcg
     8821 gagtcgaatc gaaacctcag gtttaacccc gctcacccgg agggggaggg tcgggctgtg
     8881 ctgctgctgt tggcacctgc tccgggacaa gctcggagca ggtgcccagc gttactgctt
     8941 gatggtattc acagagccga tgtggtcgtc gatgtagtcg atgggcatat acaccgccgt
     9001 gctcgtgctg cccgtgccgg agcccgcggt gtgtatgcca acacccgtga tggtgctcga
     9061 ggtccccggg taaaggaacc acggtcctcc gctgtcgcca ccttcactga tgtctgcctg
     9121 ttgggtattg ctcacccgga tccatccggc gacgccgtcc cgtgtcgcgt tcccgttgac
     9181 gatctcgcca caggtgatgc cggttgcatc gccggacttg cagacgatca ttccggcctt
     9241 ctggttcagg aaggtcatga tggatgtcat gcggaacgtg ccggccgccg ggaattccgg
     9301 aatgccgttc ctatcttcat aggcgatcgt gttgtccacc gtgaggccgg tcgtgtccca
     9361 gatctgatag tcgtacttgt cgcttatacc atccgcaccg tccggcggtg catctctgtg
     9421 gggtttgtta atcaccggcc ccgacaaggt cacggagtga tcaccgaagt cgacatacat
     9481 ggtatccggg caatgcccgg ccgtgagaat gccccttttc gccacaccgt tcaaggtaaa
     9541 gcttacagca tacccaagcg agcagtagcc gccagcacct cctggcgacg taaagatcgg
     9601 gttacccccg tacagccggt ctccgggctg cacaccggtc ggcagcgcct cgattttcgg
     9661 gatgagcccg acctctaccg tcgtatcacg cctgcgagct cgcggcagga cggactcgac
     9721 ccgttcgacg tcactggggt cttcgacgat gacggagaat ctttgggtct cgagattgta
     9781 ttccgaggtg aaagggatgg aaagctgacg aagcgccgca ttgagctggt cgaggctcct
     9841 cgcagtcgcg ccccgagatc tcttggcacc cctgagcttc acatggggac gaagctgggg
     9901 atccaatgaa tccaccagcg ccttgcggct cttattgcta ttgcctgcga acctgacgat
     9961 aactcgatac cttggcttat gcgtaatgta catgtcagcg taagccgggt cgttctcttt
    10021 gttgaggcgg tcggaaagct ccgccacctt gaactgtagg tcgagccgct tttgcgcctc
    10081 ggcctggtcg acactgtaag cttccatgag atgaacaacc gccgggttgg gcggctgatc
    10141 ctgggcgtgg gccgcaaaag agatcgtcat cacggccaaa agcagacaga aacagcttct
    10201 cttcatttca acctcctaag ccaaatagta ctttcgggca gtaagatggt attggcttca
    10261 ccaactcggc agatgacaaa cggatcccac ctccaggaat gagggtttcc tcgccgatga
    10321 ggaagcggcg gccctcaaag gagaccgcca ccacgtcatt tcccctcagc tcgggcttga
    10381 cgccgggagg gaaaacggcc gttgcgcgct cgctcccctg gatggcgacc gtgagacagc
    10441 cgtcaatgac ttcgaggcgg ccagggctcg caaggctgat gacctcaaca ggggacctgg
    10501 tttccgcaat gaagggcata gaggtcggct gttcaccggc cggcatgggg ctcgacgtac
    10561 tgtttccccc tggaggaggg gtggctgcaa gcccagaatc agaggaaggc gggtcttgag
    10621 ccggcgtggc accagtagcg ctcacctgtg gtgcctcccc gcgcctgtcg ttattatcaa
    10681 cgatcggtgc gcacccgaag gccatcacca atagagtccc caggatgaag atatgagtga
    10741 ttcgcatcag agcctccagc cctcaggcca cttcccaccc tgccgaattc gtacggcgct
    10801 gtcgccgaat cttcttcaac tctgcaatcc agatgccatc ctactcaagg gagagggcgt
    10861 cgccggctga acggccgtct ccatcctacg gggaatggcc ttcattcctg aagaaacgga
    10921 gaccatttcc tccgaaaccg ccttcattcc tggagaaacg tccgccgttc ccacggaaac
    10981 gaggatgatt tcgggtgaaa cggccgtcct ttcgggagag atggccacga ttcctcgggg
    11041 aacggcgggc attccccgag gaacggcgat catttcggcg ggaatgacga tgatttcctg
    11101 cccgctcagc aacccaccac gtaatcggtc cggtcagccc ttcgcctcca cataccggcc
    11161 gaagttgtcc aggatggcct gccaaccctc gcgctgcatc tcgggcgagt tctcggtctc
    11221 ggcgtcgaac gtctccttca cgaggacgcc gtcggcctgc tcgacgaact cgatctggac
    11281 ctcccggccg tcgctcatcc ggtactcgat ggccttgtgg ggctcgacgc gggtgtaggt
    11341 gccctcgaag tcgaagccca tgctgccgtc cctggcttcc atgcgcgacg agaacttccc
    11401 gccctcgcgc agatcgacgg agctctgggt ggtgtgccag tcatcctggg ccgagttcca
    11461 cttcttgata tcctccgggt tgttccaggc atcccagact tggttcagtt cggccttgac
    11521 gacagtctcg atggtgatct tcatgcttgc ctccttgggt gaagcccgtg agcgggccta
    11581 ctttaccctc tagtcgaacg gggagggagc cgatcgacag gcaggggcga tgactcccag
    11641 tctgctagga tctttccagg tcctgagccg atgtctcccg acaccaccac ctggaaccgc
    11701 tggcgctaca cggtctgggc gcccatctac gacgccgtgg cccgtttcgg gcggcagcgg
    11761 cggcgctccc tggcgctcct cgctctgcag ccgggcgaac gggtgctcgt cgtcggcgcc
    11821 gggaccgggg ccgacctgcc cttcatcccg gcgggggtgg aggtgctggc gacggatctg
    11881 acgccggcca tgctcgcccg ggcgcgctcg catgcccggc ccggcgtcga gctccaggtg
    11941 atggacggcc aggccctgga cctcccggac gccagcttcg acgccgtgat cctccacctg
    12001 atcgtggcgg tcatcccgga ccccgtggcc tgcctgcgcg aggcggcgcg tgtgctcaag
    12061 cccgggggaa ggatggccgt cttcgacaag ttcctgccgg acggcgcccg gccgagcccg
    12121 ccgcgccgcg ccgccaacgc cctcaccagc ttcctcttca ccgacatcaa ccgccgcttc
    12181 ggcgacatcc tcgccgcctc cggagcgccg ctccgcgtcg agcacgacga gccggcgctc
    12241 ttcggcggga tgttccgtat cctgcggctg gtgaagctac aatccgttca gtagctcaga
    12301 ttcttcaaaa atgggagatg tgccatgggt atgatcgacg agttcaagga gttcgccgtc
    12361 aagggcaacg ccatcgatct ggcggtcggc gtggtgctgg gggccgcgtt cgggaagatc
    12421 gtgacgtctt tggtcgatga catcatcatg cctcctatcg gcctcgccct gggaggcgtc
    12481 gacttcaccg atctcttcgt caacctgggc ccggggttct acgcctctct ggaggaggcg
    12541 aaggcggcgg gggcgccgac gctcaactac ggcaacttcc tccagaacat cgtcgacttc
    12601 cttctggtgg cgctggcctt gttcctcatc gtccgggtga tcaaccggct gcgacggagg
    12661 agggaggagg ccccggcggc gccggccgct cccccggaag aggtggcgct gctgcgcgag
    12721 atccgcgact ccctccaacg ccggcccgtg tagcggtcca gcgacgccgt cacatcacgg
    12781 gcgggccggc tcgaacagct ccacggggtt gccgtcggga tcctccaact ggatctgctt
    12841 cccgccggga ccggtctcga tctcgttgcg gaagcggaac ccggccgcct tcatcgccgc
    12901 cacccgggac ggcaggtcat cgacctcgag caccagccgg ttccagccgc ccggctcctg
    12961 ccggcgaccg tccggcaggg ggcgggagcc ggagctctgg ggaccgctca gccagaggga
    13021 gagattgccg ttggtgacac tggcgaacgc cggccccggt cgctgctcga gcttgaagcc
    13081 cagttgcgta taaaaggcca cggaacggtc gacgtcctga cctggtagcg gacccccacc
    13141 gcccgcgcgg cgggtggcgc gggctcgccg gcctgacccg cgaagccccc tatcaccagc
    13201 agccccatcc cactcagtgc cgagacgtat ctcttcaaca tgggtctcac ctcctgatcg
    13261 cgccgtgcgg gacggcggcc gtggcggccg tcccgcggtg tggagctcat tgtgagaccg
    13321 ctggcgcggc cggtattgga aataattgac ccggccggcg atcagccgga cagggggatg
    13381 tggttcggca ggacctgccc ggtccgccgg cggaggtagt ccgcggggct gagcccggag
    13441 aaggccagaa agtcgcggat caggtgggac tgatcgaagt acccgcagtc caccgcgatc
    13501 gacgcccagt ccggggccgc ggtctgggcc agcgcccgcg cgcgctggaa tcgcctgacg
    13561 cggcagtaca gcttcggcgt catgccgatt tcggccgcga acacctggat caaccggcga
    13621 tggctcaggc cgacacgctc cgcgacttcg cgcaccctca cctccgagcc tgtctgcccg
    13681 aacgccccga gcgccgccgg gaccgcgcca tggcgcgctg gtgagtggat gaggcgcgag
    13741 aggagagcct gctcgagcag ggagaaccgc gccgcggcgg tggcggcggc gcagagccgt
    13801 tcgcgaagct cggcggcctg cgctccccag agccatccaa ggtcgacgtg cgcatcggcc
    13861 agctcgtccg ccggtaggcc gaggaacggg aacgcgccgc ccggcttgaa gtgcacgccc
    13921 aggatcgacg cgtgctggaa cggatcgatc acgaaaaatc ggctgtaggt cccggagacg
    13981 actgccccgg agtaccgcgt gcagcggtca ggctgtgagg agtcgtaggt ccggatctcg
    14041 tcctcgcgaa gattgatcac cagctcaatc gtcccgctcg gcaggatccg ctccctcgcg
    14101 tgcggcggcg catcggagca cagccagaag cgctcgatga agctgctgag aggagccgcg
    14161 ggagtatggg atcggtagtg catggcagga ctacatcgac gcgtcctgga cgatctccca
    14221 gccgttggcg ccgcggcgca ggacgatcag ggtgagcccc tcggcgtcct tgcgctcgcc
    14281 gccctcggga tatgagagct tccagcgggc ggcgacggag acgccatgga cacggctcgg
    14341 ccgggcgccg cccagcatgg tgacctccgt gcccgacgcc ggccgggtct ccagaacctc
    14401 cagcgtcagg gtccccatgg cctggcggtc cggatagcgt ttccggtagc ggtcgaggac
    14461 ggcctggcgc ccctgggtga cgccactgga ggtgaggaag gtggcgtcgt cggcatagac
    14521 cgaggtgaag gcctcgaggt caccccgact ccaggcctcc gcctgcttct tcaacagagc
    14581 ctcgacctcg gccacgtccg tcttcgtgcg ccgctcggca gcgatgcggt cccagaggcc
    14641 ggggccgaag tggccgacat agcggtgggg ctcggagaag gcgggatcgc cgagcacctc
    14701 gtcggagccg gcgaagcggt ggccggtctc ggtgagccag gtgaagaggc ggtcccactg
    14761 cgcggcgctc acctccgtgg catggaggag gaggatctgc ggcgtctgcc ggccgaagag
    14821 gcggtcgccc acggcctcgt ggccgcggat ctccaggtgg agcgactcgt ggtactgctc
    14881 ggccacccgg tccatcgcct gccggtcccc cttcttccgt gcctcgaccc agggctcttc
    14941 gaacgaccag tcctggttgt ccagcgtcac cgggaggttg cgctggccgg actgagcgag
    15001 gtaggcacgc atggcgtcga gcttctccgg cgtatccccc tcgcgcagga aggggaaacg
    15061 gaagaagcgg acggtgcggc cgctgggctc caggagcgct gcgacctgct tgcggccggc
    15121 ttcgacgtcg gcgatgtact ccgccggcgc ggtgcgggtg tagctgaggt gcttgtagga
    15181 gtggttgccc agctcgtgcc cctgttcgag ccacatccgg aggagctcga cgtccttctc
    15241 tccctgcacc cggtcccagg tgacgagccc gacggcgcgg atgccgtgct tgcggagcgc
    15301 caacagcatc tcgcgggtga tccgctcgcg ctccgccggg tccgggtgga gcttggccga
    15361 cacgatcggc aggtcatcca ccgtgatcac caccggccgg cgcggatccg agcccggcac
    15421 caccgtcgcc gatgcggaac ggaggatcga gcccgagccg gcgaagacca ggaggaaaga
    15481 gaggattgcg aggcggcgcg tgggagtgtt gggcatggag acctttctag aatccttcgc
    15541 ccgccaccgg aaccgccggc accagcggcc ccagcggcgg atctcccttg tagaggaccc
    15601 gctccaggaa cagccccgac ggcggggcgg tccactcggc ggggagacct ttgccggagc
    15661 ggggggcctg gccggcgagg agcttctcga agccggcgag atcgaggtct cccccgccca
    15721 cctggaccag cgtccccacc acccgccgca ccatcttcca gaggaagtgc gaggccgtga
    15781 cccggaccag gatcagggcg ccgtcggtct tcacctccag gccctccacc acgaccaggg
    15841 tgctggtctg ctccgccggc ttctcacaga agagccggaa atcgtgccgg cccggcagga
    15901 gggcggcggc ctcgctcatg cggcggacat ccagcgggcg cttgacccac cagacgaaac
    15961 gcttcgccag ggccgtgcgg cggcggcaga tctggtagac gtatgagcgg gagatggcgt
    16021 cgtgccgggc gtggaagcgg tccggcgccg gggtcaggga gaggacgtgg atgtccggcg
    16081 gcagcaggtc gttgagctcg cggcggaacg gctcgggatc gatcggccgc cgcagccgca
    16141 ggtgggcggc ctgcgccagg gcgtggacgc cggcgtcggt tcggccggct ccgccgatct
    16201 cctggacggc gccgccggtc tcctcgacgg cccggcgcaa ctcgccggcc accgacttgg
    16261 cgttctgctg ctcctgccag ccacggtagc gggtcccttc gtattcgagg gtgagacggt
    16321 aggtcggcac ggcgtcctac agcctcacct tccgcgacac catcgtggtg tcgccgtcct
    16381 ggaaggtgga ggccgtggcg ggacgggcct tcaccagcgc ctcgccgccc gccggcaagc
    16441 ccggagagcg gtccagcacg taccagtccg cgggggcgga cgcgcccagc acggcctgca
    16501 cctcgatccc ctggggtggc acggagagga aggtcagagt gcggtaggcg ggggcctccc
    16561 gcgagcccgg ctggggtccg ccgcggcccg tgagctcgtc caggcgcagg gggatgcccc
    16621 ccacctccat gctttcgacc tgcgcggccg ccggcatgat cagcccggcg atccaggcgc
    16681 ctcggggtga ggacagccgg aagcggaccg tccgcttgcc acccgtcgcc gtattctcca
    16741 ggaccgtgag gtccggcgcc ggcagggcgg ccgccggagc cggcgcggcg aaggcccgcg
    16801 cgaacggcgg cgaccagggg aagggctgga cggcgctgtc accgaacttg gccccctgcc
    16861 gcaaggactc ggggaagggg ggagcgaacc gcgccagcca gcgggcctgg cccgtgtccc
    16921 cgtccaggtg gtactggagg gagaccggct ggggcgaagt ctccgtgaag accggcgact
    16981 ggcgccccac catcaagaga acgacggcga ggaccaccgc gcccagcggc acccagcggc
    17041 gccagagcgc cccgctcccg gggacgaggg gcgccagagc ggtcagcagg agggtgagga
    17101 ggacgccgat caccaggaac gccccgccgc cgaggccgtc gtagagcggc aggatcaccg
    17161 ggaaccagag gagcgccgcg accaccagcg gcaggatcgc cgccagcgcc caccggcccc
    17221 tcgggagaag cgccccggcg agacccgcga ccagcgcagg gacaacgaaa agatagctga
    17281 tgccggtggc cagaaaggcc accaccaggc ccagcaccga ccaccagagc cagacgccgg
    17341 cccacaggcc gccgaggccg gcccgccgga agaggcccgc ggccaacagc accacgccca
    17401 ggaccagcag ccagagggcg gccaggtacg gcagcgggtt cgccagccag gccacgttca
    17461 tcgcgcctag cgcccgcagg gcgaattgca gcaggaaggc cgcgagtgcg cccgcgatca
    17521 gcaccaggag gaagccgagg acgccggcga cgacggcccg gccggtgagg gtccggctcc
    17581 gtaccgaccg cgccacggcg gccaccacca ggatcagcgc cagcgccgcg agccccaggg
    17641 tccagggctc cggccaccgc accacgaaca ggctcaaaac gtcgaagaag acggcgctgc
    17701 cgcgcggcgg gttggcgatg tccgcctccc ccaggccgcg caccgccgcc agggcgttgt
    17761 cgccgtggtg ctggaggctc gccggcgagg cggtctccag gttgtccagg gcggtgtggt
    17821 agtgggccgg cttgcggatg taggcgaagt tgagccccgg caccgggtcc ggacggcgct
    17881 tgaagaccgt caggtccgtg tcgttgggga gctggtcgta gatcgtggcg aagaccgagc
    17941 tggtcaccgg ccggggcgcg ttgcgggcat agctcgagac cagccaggcg ttggggccgc
    18001 tggtctcgaa catcaggctc ggtccggcgc tcccccgggc ctccaggttg atgatcgcct
    18061 tcacctcgcg cgcggccggg tggtagtcgg cgaaggcctt ggcccccagc agcccggcct
    18121 cctcgccgtc gtcgaggacg aggaggacgt cgttcctgag cggcggcccg gccttgagga
    18181 tgcgcgccac ctccaggatg gcggccacgc cgctcatcgc gtccgaagcc ccggggcctc
    18241 cggccaccga gtcgtagtgc gccatcatca tcacgacacc ggtggactgc cggccgggca
    18301 ggcgggcgaa cacgttctcc acccaggcgc agttgccgac ccctccgagg ttgcaggaga
    18361 aggcccgctg cacgtccggc tcatagccga gggcgcgcag ccgggcgatg atgcggtccc
    18421 gcaccagcgc gttggccggg ctgccgacgg gatgcggccg ctggtccccg aggagctcct
    18481 gctgcacctg ccgcgcccgc ccggcggaga actccgccgc cggcgcgtcc gccggccgcg
    18541 gcgccggcgc ctgcgaccgc atcacgacca gcgccaggac cagcgccagg agaacgagcg
    18601 cggcggccag cccccagcgc ggcgtctccc cgggttctac ggtgttgctc gtctccatcc
    18661 ggtcaaccat cggccccaac ctcctccaag agaacaggtc catgcgaggc aattcgcgcc
    18721 gggattctac cagtacatcc ccggggggct ccgcgcggct cttccacagc aatcggttga
    18781 cgacgcgccc ctcctcctcg tactctcgcc gcgcatgaat ctcagccagg aagagctcct
    18841 gttcgggcgc atcgcgctcc actacaagct cgccagccag gagcagctca acgctgccgc
    18901 cgccatcctg accagtgagg gagggctgcg cgggctgggc gagatcctgg tcgaccaggg
    18961 aattctccag cagatccagc tcgaccagct ccgggcggtg cagcgggact acgtcgccaa
    19021 gaagcagggg cagaccgcca ccgcggcctc ggctcccatg accatcgagg ccgcggacgc
    19081 cgccccgctg ccggagtcgc cgatgcccac ggaggctccg gagccggcac cgccgcccga
    19141 gccgtcgtgg cgcacggcgg agcgcccgct cctggagctg ctcgtccagg cgatccgcca
    19201 gggggcgagc gacatccacg tgcactgcgg cgccaccccc aaggtgcggc tccaggggac
    19261 gcttgaggag gtggactcca cgctcctcac cgccgccgag accgacagct gggtgcgcga
    19321 cgtgctgacg ccggagcaga tcgaggccct ggagaagcac ggtcaggtgg acttcgccta
    19381 caacatcccc ggcgtggggc gcttccgcag caacgcctac cggcagcagc gcggcgtcga
    19441 cgcggtcttc cgggcgatcc cgccgacgcc tccgaccctg gagggcctcg ggctaccggg
    19501 gtccctcgcc cgcttcaccg acttccatca gggcatggtg ctgatcaccg gcccgtcggg
    19561 ctgcggcaag tcgtcgacgc tggcggccct gatcaacatc ctcaacgagt cgcggccgga
    19621 ccacgtcatc accatcgagg acccgatcga ggtgctccat ccgtccaagc gctgcgtcgt
    19681 caatcagcgc caggtggggc gccacaccgg ctccttcgcc cgtgccctcc gggcggcgct
    19741 gcgcgaggac cccgacatca tcgccatcgg cgagctgcgc gacctggaga cgatccagct
    19801 cgccctgacc gccgccgaga ccggccacct ggtgctggcg accctccaca ccagcaacgc
    19861 catccgcacc gtcaaccgca tcctgggcgt cttcccgccg gcgcagcagg agcagatccg
    19921 caccatgttc tccgagtcgc tgcgggcggt ggtctcccag cgcctggtca accgcgccga
    19981 cggcgcgggg cgggtgccgg cgctcgaggt gctggtggcg aacaaggccg tgggcaacct
    20041 gatccgggac aacaagactt tccagctccg ctcggtgatc cagaccggcg gctcgcaggg
    20101 catgtgcctg ctggacgcct cgctccagga gctggtcaag aacaaggtga tcacccgcca
    20161 ggaggcgctg cggcactgcg aggagccgca acgcttcgcg ggctgaccgg agcccccgcc
    20221 atggcccgac tgcacgagct cttccttcac ctcaagaacc acggcgcctc ggacctccac
    20281 ctggcctccg ggctcgagcc gcgcgtccgc gtccacggcg agctcgaggc ggtgccgggc
    20341 tggccggtgc tggacaacga ggccatgctc ggcctgctgc gcgagatcgc ctccgacgag
    20401 cagtgggagg agtaccggcg ctgcggcgac ctcgacttcg cctacggctt cgaaggggtc
    20461 gcccgcttcc gcgccaacta tctggagcag tcgaacggcg ccggcgcggt cttccgcatg
    20521 atccccgagg agatcgtgcc gctcgagaag ctcaagctcc ccaaggcgat cgagagcctg
    20581 gcccacctgc agcaggggct ggtgctggtc accggcccca cgggctcggg caagtccacc
    20641 accctcgccg cgctgatcga ctacatcaac accacccgcg ccgcacacat catgacggtc
    20701 gaggatccca tcgagtacct gcaccgggac caccactcga tcgtcaacca gcgcgaggtc
    20761 gcggtcgata cccgcacctt ctcgcacgcg ctgcggagcg cgctgcgcca ggatcccgac
    20821 gtgatcct
//
LOCUS       DASPJI010000430        20185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_141597, whole genome shotgun sequence.
ACCESSION   DASPJI010000430 DASPJI010000000
VERSION     DASPJI010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 20185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20185)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20185
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141597"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VEL74_11580"
     CDS             <1..450
                     /locus_tag="VEL74_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63213.1"
                     /translation="MLALLPVGWLFSLSSRFLMSAVLFHVCLWAIGLLLAWRFLGPAL
                     RASGAATGALLLWLGLFFLVSLQSTTLLRPVLARDTGAPLFVEGKKSFFEHLGDAGRW
                     DEKKERERAEALKKEKAKQEALKKEREKKQQEAHAKKKGTAKKVEAK"
     gene            534..695
                     /locus_tag="VEL74_11585"
     CDS             534..695
                     /locus_tag="VEL74_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63214.1"
                     /translation="MRKKLIKLTLALTVLAAASLMPAPAAEAACNTYCCPDGRCVTCC
                     RPCAIRCAP"
     gene            764..2806
                     /locus_tag="VEL74_11590"
     CDS             764..2806
                     /locus_tag="VEL74_11590"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HYG63215.1"
                     /translation="MNTVARLLLIPVTAAALALPAAAQDEPAAPPRLVVFDPTNMDTT
                     ADPCQDFYQYACGGWNRRNPLPADRSTFSTFSQLGERNELILRDLLEKAAAPAPGRGA
                     VEKKIGDYYASCMDEKAIEAKGIAPIQADLDRIARMKDKAALAKTLAHFHLQGTDAAF
                     RLTASQDAKDSTRFIASAGQGGLGLPSSEDYSAKDPKSVEIREKYVAYLQKALELLGE
                     PADRAAAQAKAVMALETALASGALSPVERRKPENRYNLVKTGELAKLAPAFDWQAYFA
                     AVGLSNLAEINAAPPAYFQRLGEQVANAPLDDWKAYLRTRLVSESAPALPKAFVDLRF
                     DFFERTLRGVQQQEPRWRRCVDAVDRDLGEALGQRFVETAYGEKSRERMAELITALRK
                     AMSREIQSLEWMGDATKKEAQAKLAKMGPQIGYPKTWRDYSALEVVRGDALGNRRRAR
                     GFDLRRRLDKIGKPVDPNEWSMSPPTVNAYYRPSMNDINFPAGILQPPFFDPNADDAV
                     NFGAIGSVIGHEMTHGFDDAGRKFDAQGNLRDWWTPEDAKEFEARAACVDRQYSGYSA
                     VDDLKVNGKLTMGENIADLGGMRVAYMALQDTLNGKEPPKKDGFTADQRFFLAYAQVW
                     CFDATPEARRQRALTDPHSPGRYRANGAVSNMPEFHKAFGCKAGQPMVREEVCRVW"
     gene            complement(2813..3082)
                     /gene="xseB"
                     /locus_tag="VEL74_11595"
     CDS             complement(2813..3082)
                     /gene="xseB"
                     /locus_tag="VEL74_11595"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HYG63216.1"
                     /translation="MSEPTKGAVAGGSGEEPGFRQAMDELEGILERIEGEEIDIDRLA
                     EELRRAAFLLDLCRGKIRKAEVEVTQIVQSLEETDRPAGNSGNED"
     gene            complement(3079..4452)
                     /gene="xseA"
                     /locus_tag="VEL74_11600"
     CDS             complement(3079..4452)
                     /gene="xseA"
                     /locus_tag="VEL74_11600"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HYG63217.1"
                     /translation="MNLNLPLFEPTYSVSRLCGEIRDFLGEAFSAVWVAGEAQRVRPS
                     ARGHLYFELVEKGDRDEITGKLDAVIWKTDFQKVRRMLAATGQDLKDGMQIRVRGSLD
                     FYGPGGRLQLCIREVDPTFTLGLLEMRRRETLAALSAAGLLERNRTLELPELPLTIAL
                     ITSHGSAAYHDFLSGLQESGYGFRVLFVHASVQGKEAEREVVSALRSLQGLEIDCAVV
                     IRGGGSRSDLAVFDSRVIAEAIAQAPFPVLTGLGHEIDQSIADLVAHTSVKTPTKVAE
                     LLVGRVAEVEMALLEVRRCLLREALEPLRSGREALGRAEKGVQLARMRLASAGARIEE
                     HARALGRLGRSALRQTRRRQDELRRRLADLGPRQVEAEERERRGLAGRLTSVSRGHLR
                     EAQATLSGLERLCQQLGPERTLERGFSITRDAAGKVLRHPGQVTSGDRLTTRVAGGGV
                     VSRVEES"
     gene            complement(4472..5023)
                     /locus_tag="VEL74_11605"
     CDS             complement(4472..5023)
                     /locus_tag="VEL74_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63218.1"
                     /translation="MAPRRMMNRLFYFTIEDESILAESFAQLDKETYAITYKVVGTED
                     VFVTTSETKDAMDRNDIPYNLLAEEDGAKISLYHSPLSREELGEFEDAVKALALACRA
                     IAMACVGVNGESNLGFDLTNGGAKHYTYFTAPAGHTFIWRLFHERDDAVDFLSRLTAG
                     EKKAVEWAESIPLGSTKELKSYH"
     gene            complement(5305..6495)
                     /locus_tag="VEL74_11610"
     CDS             complement(5305..6495)
                     /locus_tag="VEL74_11610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HYG63219.1"
                     /translation="MGASDGYTHVHLKEGRDRPVREGHPWIFSGAVARVEEEDAAPLA
                     QVFDAAGAPLGVGFYSPRSQILVRMLDAGAATADRAFFAARLAEAVALRQSVLPPDTT
                     GYRLVNAEGDGIPGWTVDRFGDVLVSQVTVSGLEAVRSEAYAALAETLPGLAILQANG
                     LPVRSAAEGLTGEDEVIAGEPPAETLFTESGLTFTAELAGGQKTGFYCDQRENRRLAG
                     KMAAGRDVLDLFAHSAAFGLYAARGGARRVVHVESAPRLLERGRRHFQLNGLDPDAAA
                     EWVKANVFQDLRERQDTYGLVICDPPPLVRKRPDLDAAARAYKDLNRLALQRVAPGGF
                     LLTFTCSGAVDAKLFRQILFAAAVESGRRVTLLTPLAAAPDHPIAITHPQGEYLKGWL
                     AAVS"
     gene            complement(6615..8738)
                     /locus_tag="VEL74_11615"
     CDS             complement(6615..8738)
                     /locus_tag="VEL74_11615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG63220.1"
                     /translation="MRHLPKPRFLTAALCAALLLPPALPAAAQGFDAATDVVVVEVPV
                     QVVRDGAPVRGLTAADFEVYEGRKKQEVTGFEVIDLAAVPGAGARPALSASARRHFLL
                     LFDLSNSESKSILKARGAAREAVLGKLHPSDLVAVATYSSRGPQVVLGFTPDRSQVER
                     AIETLGVPELVDRNADPLKLTAAEVRSVVASGAAREREAMVLEYIEGLERDSRKGEVE
                     AQRARVSAFTRSLADLAKLLNSVAGRKHVIFLSEGFDSSILTGTVDRAEQERMQEAVA
                     SGEFYNVDSDERYGSSQAGNALEQMLEEFRRADCVIQSVDIGGLRGEGDLGPRRASGQ
                     DALMAMAKDTGGEMYRNFNDLGAAMDQMLQRTGVTYVLAFQPERLKRDNSFHRLRVEL
                     KNAPRGTRVVHRPGYYAPRPFSEQSALEKLLQAANLLTSPGDTGTIPTAVLAAPFRPA
                     AGEKAYVPVLVEVDGKALLAGKQGSSIPTEIYVYAFDGSGAVHDFLTQTVGLDLGKLE
                     PALRQTGFKFFGHLDLLPGDYSVRVLVRNGVTGQTGLKAVPLTVPAFAQAGPVLLPPL
                     FPEPPTKWVLVPETLQPGETRPDYPFMLQQQPYVPASRPTVAPGQEVQVSLVGYNLGD
                     GELQAVARVTTPDGKEVAPATLRLAHRERGTGSAPDRWTAAFQAPGLQPGEYLLSVQL
                     TGPAGSAPAAVLPFVVGSAARGGRS"
     gene            complement(8735..10972)
                     /locus_tag="VEL74_11620"
     CDS             complement(8735..10972)
                     /locus_tag="VEL74_11620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG63221.1"
                     /translation="MSARIRHFLSLVLPLLLAAPAAPQATASQAGAQPDRQVFQDEAR
                     VFAVEVPVHVVGRDGQPVRGLTAADFEVFDEGKRQTLSNFEVVDLERLSRMEPAAGAA
                     AGAPPLHAAARRRLLLLFDLSFSTPASVVKARQAARDFVLTSLQPSDLVAVATFSLEA
                     GPRLLVTFTPDRAQLARAVDTLGTLGETQGKADPLRFVVEQPSSVSASRTLDAETSDL
                     KRANADAALYEHLTAISLYAEKMSRSFEQSRITAMTRSLGDMARWLDNVKGRKHVLFF
                     SEGFDSRLFLGRTETDPETVTDQDYAMHGRLEYVDSDSRFGNTALQGDVQRMLEEFRR
                     ADCVIEAVDVGGLRNQADATEAPRRAGKDVLFYMANETGGELMEDSNDLGRHLGRVLT
                     RTSLTYLLTFQRSDLEPDGAYRRLRIKVKGLPPGARVSHRSGYYAPRPFQSLHPLEKN
                     LLASDGIANALPRRDLDLRVLAAPFRATPDRAYVPVIIEVGGGALLAGRQGDQLNVEL
                     FAYASDEKGEMKDFFTRTVGLDMGKGGKSRDAVRDTGIKYYGHLDLPPGRYRVRILVR
                     DAETGKTAVEAVPLTVPDWRDARLQLLPPLFLDAARWLLVREPGDGDGAVGEGSVVYP
                     FTVNGEPYVPAARPRLAAGDEARLCLVAYNLGEGNPLLHGEVLAADGQVVTGPAGDTA
                     GGRLSGVERTATGLAGVDKLLATFRPAGLVPGDYTLRVRVTDPVTGAPEISSAPFSVV
                     ERGDS"
     gene            complement(11009..12142)
                     /locus_tag="VEL74_11625"
     CDS             complement(11009..12142)
                     /locus_tag="VEL74_11625"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG63222.1"
                     /translation="MFRKAGLLLSSVLLASAVLTAGCSEYKRFDSAAYLRGEYTQRIG
                     PQAAAALEVPFEVSPELRAELQGRLRRTSGERRNLKQVIDFIFDRLDLQYELAPTRNA
                     AETFRTRRGNCLSFVNLFVGIAREQGLAPFYVEVTDYQKWSSREGMVVSQGHIVAGMY
                     LEGQLETYDFLPYRPKGYKGFKPIDDLTAAAHFFNNLGAEALLAGDTESARRLLATAT
                     EIAPRFEKALNNLGVLLARGGEPERALDLYRRGLEAAPDNPTLLTNLVRLYHQLGRTD
                     EAASLAARIEEGNSSNPFFFVYQGEMALGRGDTTAALDLMARALRQDSELPEVHLGLV
                     KVYLALGDMEKARHHLDRALKLDATHPEARRYAGMLGRAARSE"
     gene            12501..15512
                     /locus_tag="VEL74_11630"
     CDS             12501..15512
                     /locus_tag="VEL74_11630"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HYG63223.1"
                     /translation="MKSTNFWGRALAVAAVLLLVAGGSAFAQLQTGALFGTAIDDQGN
                     VLPGVTVTLTGAAGPPQVQVTDAQGRFRYPTLGPGSYSVKAELEGFSPVDYPNISINI
                     GRNTEIEITMNSAVKEVITVTTESPLLDQRRISAGATIEQTELEKIPTSRDPWAIMQS
                     TPGVLTDRINVGGNESGQQSQYVGPGSSGDQAVWSVDGVTITDMGALGSSPAYYDFDS
                     FEEIQITTGGSDTTVATGGIVLNMVTKRGTNEWRGSGRYYVADNDWQSDLDFDENDLA
                     QAGPWSNDTPQEAFNQGNRIVKVEDYGVELGGPVVKDRFWVWGSFGKQEVGLLTIDDV
                     SDFTELETMNVKLNAQLSQSNSATASGLQSDKIKIGRNASPTRPQPTTWNQSKFGDDP
                     TTWKVEDTHIFSPSFYLTGMYSVVNGGFQLVPQGGMDINATWDEDFVWQNSFLLYQTP
                     RPQEQFKADASTFVNTGSVAHELKYGAGYREADVSSLSRWPGFGIQLNFYQSLGNEDP
                     ADDVPYNVIELTRDALPSSHLEYTNFYLQDTLSAGNLTANIGLRYDRQEPTLDRVTVR
                     ANPAFPDLLPEATFEGGSGGFSWESITPRLGLTYALGQERKTLLRASYSQFADQLGTG
                     IGTWISPLYPGTYLYLYYDDQNGDGIAQQGEVLANTPCNACSVPYNGTYNPLTRGLLV
                     SNGVDPDLKPPMTHELLFGIDHAVRPEFLIEFKASYRLLTDLLEQERLVFDGDIDYNS
                     IGRLHRADDYVPVTTTTTLPDGRVQESTYYVLRDGVTTRNGFWLENGDREQNFFGASL
                     GFNKRLANRWMLRGNVSWQDWEWDVPNSEREDPTRLLGGGPLGSGSQDGDPVLQGSGS
                     GSGAKGGVYINSNWSYSLTGMYQIAPERPWGFNAALSAYGREGYPNPYFRRVDRGSIS
                     DFLARNTSILVAPDQDSYRLDDIHMMDARLEKEFTVSDLGLTLTADVFNVLNQSYVIQ
                     RAHRLNRANGDFVREITSPRIFRLGLRISFR"
     gene            15669..16592
                     /locus_tag="VEL74_11635"
     CDS             15669..16592
                     /locus_tag="VEL74_11635"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG63224.1"
                     /translation="MVLKMLRRRRLPLAAPSAVIVAIAAFVLSLAAPGTAQEWKGKGR
                     LQGDVLDAEGKPVAGAKVTLRPGAPPVDPAAGGPAPITTDKRGKWAILGLDNGPWAVL
                     IEKEGFVRSEGQVQVNEYAPAKPIRIALNPAAQETRQAAAANAAGEEALAALEAGNAK
                     LQAGQFAEARAEYEKAMATLEPANHPAVLRGIARTYYQEKKLDQAIASLQKALELKPD
                     DAESRKLVANIYVDKGIEAYNATQVPEAAQQFSLAIENDGTLADAYYYRGLALMAQTK
                     NAEAKADFQKLLELDPNYPQAADVREFLQSL"
     gene            16621..18762
                     /locus_tag="VEL74_11640"
     CDS             16621..18762
                     /locus_tag="VEL74_11640"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYG63225.1"
                     /translation="MRHPLMPALALAAALMAAGSLPGCAPADDRGTAEAAPASSAPLP
                     ASARGADVILITLDTLRADTLGFAGHPRAATPVLDRLAAAGRVYTDAHAHNVVTLPSH
                     ANILTGRYPYQHGIRDNSGFTLPASVPTLGTMLRQAGYRTGAFVAAYPLDSRFGLDQG
                     FDLYDDDYPRGSNPADFVVAERRGDQVIAPALRWWRSQRGHRRFLWVHLYDPHAAYAP
                     PEPFASRFRDNPYLGEVAATDSFLAPLLQPLLGGKEPPALVVVTSDHGEALGEHGELT
                     HGLFAYEATLKVPLVLWGAGVRPGKDTRPARHIDIVPTVLGALGVKSPSGLPGRSLLG
                     PASQTDTSYFESLSTALNRGWAPLRGTLRERRKYIELPLPELYDLARDPGEQKNLFGE
                     ERRAANALRQALPEESVWPPRKGQVSPEEEARLRSLGYMTGDAGDKASFAPADDPKNL
                     VALDRQLHQVVDAYSRGRYEEAARVARQVVAARPTLAEGYEHLALALRQLERQDEAVT
                     VLRQGVTRIGQGGGRDSLRRQLGQALAEAGRPAEAVEVLAPVASGSSDASVLGAYGIA
                     LSDAGRHGEAAAVLQRALSADPSNPKVLENLGIIALRMRQPAAARDHLRQALALNDRL
                     PIAWNTLGVALYQLEGPPAAAAALDAWERAVAIDPAQYDALYNIGLVAREAGRREQAR
                     RALERFVRTAPPSRFGPDLAKARQILAGLGA"
     gene            18765..>20185
                     /locus_tag="VEL74_11645"
     CDS             18765..>20185
                     /locus_tag="VEL74_11645"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYG63226.1"
                     /translation="MIRRLPLLSLVLLLAGAAACSDAGGGGGRGGGEVFEDAPVILIS
                     IDTLRSDRLPAYGYEKVETPAIDALAKDSILYERAYSHYPLTLPSHVSMMSGLLPPRH
                     GVRDNVGYSFDAEKHPSLARLFKEAGYETMAAVSAFVLRGSTGMGEGFDTYDDRLIPK
                     EGQSLDAVQRAGDEATRSATAWVRERAGRPFFLFLHLYEPHAPYMPPEPFKSRFADPY
                     DGEVAAADAYVGELLAELKKLEIYDKAVIVLLSDHGEALGDHGGQTHGVFLYREALQV
                     PLLVKLPGARHGGTRVAAPARLVDVFPTLLATAGLDVPQGDGASLLELLSPGAPPRQV
                     YSETFYPRIHFGWSDLASVIDGPHHYIEGPDPELYDLVKDPRELENRFGSDRRAVGTL
                     RQALAKVDRTFNPPGEVDAETARKLAALGYAAGGGPVPEGPLPDPKSQLHVVQKMEAS
                     FSAFDHGDYEKAAQGFRELVTENA"
BASE COUNT         3022 a         7247 c         7061 g         2855 t
ORIGIN      
        1 atgctcgccc tgctgccggt ggggtggctc ttctcgctga gcagccgatt cctgatgtcg
       61 gccgtgctct tccacgtctg cctctgggcg atcggcctgc tgctcgcctg gcgcttcctc
      121 ggaccggcct tgcgggcgag cggcgcggcg accggcgcgc tgctgctctg gctcggcctc
      181 ttcttcctcg tctccctcca gtccaccacc ctcctgcggc cggtgctcgc ccgggacacg
      241 ggagcccccc tcttcgtcga aggcaagaag tccttcttcg agcacctggg cgatgccggg
      301 cggtgggacg agaagaagga gagggagcgg gcggaggcgc tgaagaagga gaaggcgaag
      361 caggaggcgc tcaagaagga gagggagaag aagcagcagg aggcgcacgc caagaagaag
      421 ggaacggcga agaaggtgga ggcgaagtga gcccggcacc gccaatcccc tccgcggcac
      481 cagggttaga ggagggggcc cgaacgccct ctccctcttc tggaggttcg atcatgcgca
      541 agaagctcat caagctcacc ctggccctga cggtcctggc ggccgcctcc ctgatgccgg
      601 cgcccgccgc cgaggcagcc tgcaacacgt actgctgccc ggacggccgc tgtgtcacct
      661 gctgccgccc ctgcgccatc cgctgcgccc cgtagggcgt ccacgaccgg aatcgacgag
      721 atcgggagga ggaggggaga cggctcgcgg taaagtacgc tccatgaaca ccgtagctcg
      781 cctcctcctg atccctgtca ccgccgccgc cctcgccctg cccgccgccg cgcaggacga
      841 gccggccgcg ccgccgcggc tcgtcgtgtt cgatccgacg aacatggaca cgaccgccga
      901 tccctgccag gacttctatc agtacgcctg cggcggctgg aaccggcgca acccgctgcc
      961 ggcagaccgc tccaccttct ccacgttcag ccagctcggc gagcgcaacg agctcatcct
     1021 gcgcgacctg ctggagaagg ccgccgcacc cgcaccgggg cgcggcgccg tcgaaaagaa
     1081 gatcggcgac tactacgcct cgtgcatgga cgagaaggcc atcgaagcca aggggatcgc
     1141 gccgatccag gccgacctcg accggattgc ccgcatgaag gacaaggccg ccctggcgaa
     1201 gaccctggcg cacttccatc tccaggggac cgacgcggcc ttccggctga ccgccagcca
     1261 ggacgccaag gactccaccc ggttcatcgc ctccgccggc cagggggggc tcgggctgcc
     1321 cagctccgag gactactccg ccaaggaccc gaagtcggtc gagatccgtg agaagtacgt
     1381 cgcctacctc cagaaggcgc tggagctgct gggcgagccc gccgaccggg ccgccgccca
     1441 ggccaaggcg gtcatggcgc tggagacggc cctcgccagc ggggcgctgt ccccggtcga
     1501 gcgccgcaag cccgagaacc gctacaacct ggtcaagacc ggggagctgg cgaagctcgc
     1561 ccccgccttc gactggcagg cctactttgc cgccgtcggg ctgtccaacc tcgcggagat
     1621 caacgccgcg ccgcccgcct acttccagcg gctcggcgag caggtcgcca acgcgccgct
     1681 cgacgactgg aaggcctacc tgcgcacccg gctggtgtcg gagagcgcgc cggcgctgcc
     1741 caaggccttt gtcgatctgc gcttcgactt cttcgagcgc actctgcgcg gcgtccagca
     1801 gcaggagccg cgctggcgcc ggtgcgtcga cgcggtcgac cgcgacctcg gggaggcgct
     1861 gggccagagg ttcgtcgaga ccgcctatgg cgagaagagc cgggagcgca tggccgagct
     1921 gatcacggcg ttgcgcaagg cgatgtcacg ggagatccag agcctggagt ggatgggcga
     1981 cgccaccaag aaggaggcgc aggccaagct ggcgaagatg ggcccccaga tcggctatcc
     2041 caagacctgg cgcgactact ccgccctgga ggtcgtccgc ggcgacgccc tgggcaaccg
     2101 ccggcgcgcc cgcggcttcg acctgcggcg gcggctggac aagatcggca agccggtcga
     2161 tcccaacgag tggtcgatgt ccccgccgac ggtcaacgcc tactaccggc cgtcgatgaa
     2221 cgacatcaac ttcccggcgg gcatcctgca gccgccgttc ttcgacccga acgccgacga
     2281 cgcggtgaat ttcggcgcca tcggctcggt gatcggccat gagatgaccc acggcttcga
     2341 cgacgccggc cggaaattcg acgctcaggg caatctgcgc gactggtgga cgccggagga
     2401 cgccaaggag ttcgaggccc gcgccgcctg cgtcgaccgg cagtactcgg gatacagcgc
     2461 ggtggacgac ttgaaggtca acggcaagct gaccatgggc gagaacatcg ccgacctcgg
     2521 cggcatgcgc gtcgcctaca tggcgctcca ggacacgttg aacggcaagg agccgccgaa
     2581 gaaggacggc ttcacggccg accagcgttt cttcctcgcc tatgcccagg tctggtgctt
     2641 cgacgccacg cccgaggcgc ggcgccagcg ggcgctcacc gatccgcatt cgccgggccg
     2701 ctaccgcgcc aacggcgcgg tgtcgaacat gcccgagttc cacaaggcct tcggctgcaa
     2761 ggcaggccag ccgatggtgc gcgaggaggt gtgccgggtc tggtagagaa ggtcaatctt
     2821 cgttgccgct gttgccggca gggcggtcgg tctcttcgag gctctggacg atctgggtga
     2881 cctcgacctc ggctttgcgg atcttgccgc ggcagaggtc gaggaggaag gcggcgcgcc
     2941 gcagctcctc ggcgaggcgg tcgatgtcga tctcctcgcc ctcgatacgc tcgaggatcc
     3001 cctcgagctc gtccatggcc tgacggaagc ccggctcctc gccgctgccg ccggccaccg
     3061 cccctttcgt cggttcgctc atgattcctc cactcggctg acaacgcccc ctccggccac
     3121 ccgcgtggtg agccggtcgc cagaggtgac ctgcccggga tgtcgcagga ccttgcccgc
     3181 cgcgtcccgg gtgatgctga aaccccgttc gagcgtgcgc tccggcccga gctgctggca
     3241 gaggcgctcg aggccggaga gggtcgcctg cgcctcccgc aggtggccgc gggagacgct
     3301 ggtgagccgg ccggccagac cgcgacgctc gcgctcctcc gcctcgacct gccgcggccc
     3361 gaggtcggcg agacgccggc gcagctcgtc ctggcgccgc cgggtctggc gcagggcgct
     3421 gcggccgagg cggccgaggg cgcgggcgtg ctcctcgatg cgcgcgccgg ccgaggcgag
     3481 gcgcatacgc gcgagctgca cccccttctc cgcccgtccc agcgcctcgc ggcccgagcg
     3541 cagcggctcc agggcctcgc gcagcaggca gcgccgcacc tccaggagcg ccatctccac
     3601 ctccgccacc cgcccgacga ggagctcggc gactttggtc ggcgtcttca cggaggtgtg
     3661 ggccaccagg tccgcaatgg actggtcgat ctcgtgcccc aggccggtga gcaccgggaa
     3721 cggcgcctgg gcgatggcct cggcaatgac gcggctgtcg aagaccgcca ggtcgctacg
     3781 cgagccgccg ccgcggatga cgacggcgca gtcgatctcc agcccttgca gggaccgcag
     3841 ggccgagacc acctcgcgct ccgcctcttt cccctgcacc gaggcgtgga cgaagagcac
     3901 ccggaagccg tagccgctct cctggagccc ggacaggaag tcgtgatagg cggcgctgcc
     3961 gtgcgaggtg atgagggcga tggtcagcgg cagctccggc agctccagag tccggttgcg
     4021 ctccaggaga cccgcagcgg agagggccgc cagggtctcg cggcgccgca tctcgaggag
     4081 cccgagggtg aaggtggggt ccacctcgcg gatgcagagc tgcaggcggc cgcccggacc
     4141 gtagaagtcg aggctgccgc ggacgcggat ctgcatgccg tccttgaggt cctggccggt
     4201 cgccgcgagc atccggcgca ccttctggaa gtcggtcttc cagatgacgg cgtcgagctt
     4261 gccggtgatc tcgtcgcggt cccccttctc caccaactcg aagtagagat ggccgcgggc
     4321 gctcggccgg acgcgctgcg cctcgccggc cacccagacg gcggagaagg cctcgcccag
     4381 gaaatcgcgg atctcgccgc agagccggga gaccgagtag gtgggctcga aaagggggag
     4441 gttcaggttc agggccgggc tccgcgacgg ctcagtggta cgacttgagc tccttcgtgg
     4501 agccgagcgg gatggactcg gcccattcca cggccttctt ctcgccggcg gtcaggcgcg
     4561 acaggaagtc gacggcgtcg tcgcgctcgt ggaacaggcg ccagataaag gtgtggccgg
     4621 cgggcgcggt gaagtaggtg tagtgcttgg cgccgccgtt ggtgaggtcg aagccgaggt
     4681 tggactcgcc gttgacgccg acgcaggcca tggcgatggc gcggcaggcg agggcgagcg
     4741 ccttgaccgc atcctcgaac tcccccagct cctcgcgcga cagcggcgag tgatagaggg
     4801 agatcttggc accgtcctcc tccgccagca ggttgtaggg gatgtcgttg cggtccatcg
     4861 cgtccttggt ctcgctggtg gtgacgaaga cgtcctcggt gccgacgacc ttgtaggtga
     4921 tcgcgtacgt ctctttgtcg agctgggcga agctctcggc gaggatgctc tcgtcctcga
     4981 tggtgaagta gaagagacgg ttcatcatgc gtcgtggtgc catggcggcg ttcctccaaa
     5041 cgggatctgg gccactcgcc ctccccttgc aggtggtggg cggacccgga aacccggtct
     5101 tttttgcttt acgggtgaaa tgagcggcag gtcgagactc ggctccgcac cccgggcacc
     5161 cattgtaaca gaggggtcac ggcgggcctt cccggccccc gccgtgggct gccggcccct
     5221 gctcggctcc tttcgagtgc gctccgccct cccccggacc acgctgccag tcgcctgcgc
     5281 cgggcccggc aggccatggc ctcttcagct caccgccgcc agccagccct tgaggtactc
     5341 gccctggggg tgggtgatgg cgatggggtg gtcgggggcg gcggcgaggg gggtgaggag
     5401 ggtgacgcgg cggccggatt cgacggcggc ggcgaagagg atctgccgga agagcttggc
     5461 gtccaccgcg ccgctgcagg tgaaggtgag caggaagccc cccggcgcca cccgctgcag
     5521 ggcgaggcgg ttcaggtcct tgtaggcgcg tgccgcggcg tccaggtcgg ggcgcttgcg
     5581 caccagcggc ggcggatcgc agatcaccag tccgtaggtg tcctgcctct cgcgcaggtc
     5641 ctggaagacg ttggccttca cccactcggc ggcggcgtcc ggatcgaggc cgttgagctg
     5701 aaagtggcgc cgccctcttt ccagcaggcg cggcgccgac tcgacgtgca ccacccggcg
     5761 ggcgcccccc cgcgcggcgt acagcccgaa agccgccgag tgggcgaaga ggtccaggac
     5821 gtcgcggccg gccgccatct tcccggcgag gcgccggttc tcccgctggt cacagtagaa
     5881 gccggtcttc tgtccgcccg ccagctcggc ggtgaaggtg aggccgctct cggtgaacag
     5941 ggtctcggcc ggcggctccc cggcgatcac ctcgtcctcc ccggtgagcc cctcggcggc
     6001 gctacgcacg ggcaggccgt tcgcctggag gatggcgaga cccggcagcg tttcggcgag
     6061 ggcggcgtag gcctcggacc gcaccgcctc caggccgctc accgtcacct ggctcaccag
     6121 gacgtcgccg aagcggtcga cggtccagcc cgggattccg tccccctcgg cgttcaccag
     6181 ccggtagccg gtggtgtccg gcggcagcac ggactgccgg agggccacgg cctcggcgag
     6241 gcgggcggcg aagaaggcgc ggtccgcggt cgcggcgccg gcgtccagca tgcggaccag
     6301 gatctgcgag cgggggctgt agaagccgac ccccaggggg gcgccggcgg cgtcgaagac
     6361 ctgggccagg ggggccgcgt cttcctcctc cacccgggcg acggcgccgg agaagatcca
     6421 ggggtgcccc tcccgcacgg gacggtcacg cccttccttc agatggacgt gggtgtagcc
     6481 gtcggaggct cccaccttat gtgtaggggc gggacctgct cccgcccggg aggggtcggg
     6541 cccctcccct gcagggcgct gcggagacct cctgggcttc ttccccggat gataggcgcg
     6601 tccacctctc gccatcagct ccggcccccg cgggcggcgg agccgacgac gaaggggagg
     6661 acggcggccg gggcgctgcc cgccggaccg gtgagctgca ccgagagcag gtactcgccg
     6721 ggctggaggc cgggggcctg gaaggccgcg gtccaccggt cgggcgcgct gccggtgccg
     6781 cgctcacggt gcgccagccg cagggtggcc ggagccacct ccttgccgtc cggcgtggtg
     6841 acgcgggcga ccgcctggag ctcgccgtcc cccaggttgt agcccaccag cgacacctgc
     6901 acctcctggc ccggggcgac ggtgggacgg gaggccggca cgtagggctg ctgctggagc
     6961 atgaacggat agtcgggacg ggtctcgcca ggctgcagcg tctcggggac cagcacccac
     7021 ttggtggggg gctccgggaa gagcggcggc aggagcaccg gcccggcctg ggcgaacgcc
     7081 ggcacggtca gcggcaccgc cttgaggccg gtctggccgg tgacgccgtt gcgcaccagc
     7141 acccgcaccg agtagtcgcc cggcagcagg tccaggtggc cgaagaactt gaagccggtc
     7201 tgccgcagcg ccggctcgag cttgcccagg tcgagcccca ccgtctgggt gaggaagtcg
     7261 tggacggcgc cgctgccgtc gaaggcatag acgtagatct cggtggggat cgaggagccc
     7321 tgcttgccgg ccagcagcgc cttgccgtcc acctccacca gcaccggcac gtacgccttc
     7381 tcgcccgcgg ccggccggaa gggggcggcc aggaccgccg tagggatcgt cccggtgtcg
     7441 ccggggctgg tgagcaggtt ggccgcctgc agcagcttct cgagcgccga ctgctcggag
     7501 aacgggcgcg gggcgtagta gccgggccgg tggaccaccc gcgtcccccg cggggcgttc
     7561 ttgagctcca cccgcaggcg atggaaggag ttgtcgcgct tgagcctctc gggctggaag
     7621 gcgaggacgt aggtgacgcc ggtgcgctgg agcatctggt ccatggccgc gcccaggtcg
     7681 ttgaagttgc ggtacatctc gccgcccgtg tccttggcca tggccatcag agcgtcctgc
     7741 ccggaggcgc ggcgcggccc caggtccccc tcgccgcgca ggccgccgat gtccaccgac
     7801 tggatgacgc agtcggcgcg ccggaactcc tccagcatct gctccagggc gttgccggcc
     7861 tggctgctgc cgtagcgctc gtcgctgtcg acgttgtaga actcgccgga ggctaccgcc
     7921 tcctgcatcc gctcctgctc cgcccggtcg acggtgccgg tcaggatcga gctgtcgaac
     7981 ccctcggaga ggaagatcac gtgcttgcgg ccggcgacgc tgttgagcag cttggcgagg
     8041 tcggcgagcg agcgggtgaa ggcggagacg cgcgccctct gcgcctcgac ctcccccttg
     8101 cgcgaatccc tctccagccc ctcgatgtac tccagcacca tcgcttcgcg ctcgcgggcg
     8161 gcgcccgagg cgacgacgga ccggacctcg gccgccgtga gcttcaacgg gtcggcgttg
     8221 cggtccacca gctccggcac gcccagggtc tcgatcgccc gctcgacctg cgagcggtcg
     8281 ggggtgaagc cgaggacgac ctgcggtccg cggctgctgt aggtcgccac cgccaccaga
     8341 tcgctggggt ggagcttgcc gagcaccgcc tcgcgggcgg caccccgggc cttgaggatg
     8401 gacttggact cggagttgga gaggtcgaag aggagcagga agtggcgccg ggcggaagcc
     8461 gacagggcgg gccgggcccc ggcgcccgga acggcggcca ggtcgatgac ctcgaagccg
     8521 gtgacctcct gcttcttgcg gccctcgtag acctcgaagt cggcggcggt gagcccccgc
     8581 accggagcgc cgtcgcgcac cacctgcacg gggacctcga ccaccacgac gtcggtcgcc
     8641 gcgtcgaagc cctgggcggc ggccggaagc gccggaggca gcaggagggc ggcgcagagc
     8701 gcggcggtca ggaaacgcgg cttcggaagg tgtctcatga gtctcctcgc tcgacgacgg
     8761 aaaagggggc ggagctgatc tccggcgctc ccgtgacagg gtcggtcacc cgcaccctca
     8821 gggtgtagtc gcccggcacc aggccggcgg gacggaaggt ggcgagcagc ttgtcgacgc
     8881 cggcgagccc ggtcgcggtc cgctccacgc cgctcagccg gccgccggcg gtgtctccag
     8941 ccgggccggt cacgacctgc ccgtcggccg ccagcacctc gccgtgcagg agcgggttcc
     9001 cctcccccag gttgtaggcg accaggcaga ggcgcgcctc gtcccccgcc gccagccggg
     9061 ggcgggccgc cggcacgtag ggctcgccgt tgacggtgaa gggatagacg acggagccct
     9121 ctccgaccgc cccgtccccg tcgccgggct cgcgcaccag cagccagcgc gccgcgtcga
     9181 gaaacagcgg ggggaggagc tgcagccgcg cgtcgcgcca gtcggggacg gtgaggggca
     9241 ccgcctcgac ggcggtcttg ccggtctcgg cgtcccgcac caggatgcgg acgcggtaac
     9301 ggcccggcgg caggtcgagg tggccatagt acttgatccc ggtgtcgcgc accgcgtcgc
     9361 ggctcttgcc ccccttgccc atatcgagcc ccacggtgcg ggtgaagaag tccttcatct
     9421 cccccttctc gtcgctcgcg taggcgaaga gctcgacgtt gagctgatcc ccctggcggc
     9481 cggcgaggag ggcgccgccc cccacctcga tgatcaccgg cacgtaggcg cggtcgggag
     9541 tggcgcggaa cggcgcggcc agcacccgca ggtcgaggtc gcgccgcggc agggcgttgg
     9601 cgatgccgtc cgacgccagc aggttcttct ccagcgggtg gaggctctgg aaggggcgcg
     9661 gcgcgtagta gccgctgcgg tgcgagacgc gggcgccggg ggggagcccc ttgaccttga
     9721 tgcgcagccg gcgataggcg ccgtcgggct ccaggtcgct gcgctggaag gtcagcagat
     9781 aggtgaggga ggtgcgggtg agcacccggc cgaggtgccg gccgaggtcg ttgctgtcct
     9841 ccatcagctc gccgccggtc tcgttcgcca tatagaaaag gacgtccttg ccggcgcggc
     9901 ggggggcctc ggtggcgtcc gcctggttgc gcagcccgcc cacgtccacc gcttcgatga
     9961 cgcagtcggc gcgccggaac tcctccagca tgcgctggac atccccctgg agcgccgtgt
    10021 tgccgaaccg gctgtcgctg tccacgtact cgagccgccc gtgcatggcg tagtcctggt
    10081 cggtgacggt ctcggggtcg gtctcggtgc ggccgaggaa cagccggctg tcgaagccct
    10141 ccgagaagaa gaggacgtgc ttcctcccct tgacgttgtc cagccagcgg gccatgtcgc
    10201 cgagcgaccg ggtcatcgcc gtgatgcggc tctgctcgaa gctgcgcgac atcttctcgg
    10261 catagaggct gatggcggtc aggtgctcgt agagggcggc gtcggcgttc gcccgcttca
    10321 ggtccgacgt ctcggcgtcg agggtgcggc tggccgagac ggaggagggc tgctcgacca
    10381 cgaagcgcag ggggtcggcc ttgccctggg tctcccccag ggtccccagg gtgtcgacgg
    10441 cgcgggcgag ctgggcgcgg tccggcgtga aggtcaccag caggcgcggc ccggcctcca
    10501 gggagaaggt ggccaccgcc accagatccg acggctggag cgaggtgagc acgaagtcgc
    10561 gcgccgcctg gcgcgccttg acgaccgagg ccggggtcga gaacgacagg tcgaagagca
    10621 gcagcaggcg gcgccgcgcc gcggcgtgga ggggcggcgc cccggccgcc gcgcctgccg
    10681 cgggctccat ccggctcaga cgctcgaggt ccaccacctc gaagttgctg agggtctggc
    10741 gtttgccttc gtcgaagacc tcgaagtcgg cggcggtcag cccccgcacc ggctgcccgt
    10801 cgcggccgac cacatggacg ggcacctcca cggcgaagac gcgcgcctcg tcctggaaga
    10861 cctggcggtc tggctgggcg ccagcctggg aggcggttgc ctggggcgcg gcgggggcgg
    10921 ccagcagcag ggggagtacg agggagagaa agtgacggat ccgcgcgctc atggttcagc
    10981 tccttcttcc agggtgcgcg ttccgagtct actccgaccg cgccgcccgg cccagcatgc
    11041 cggcgtagcg gcgggcctcc gggtgggtgg cgtccagctt gagggcgcgg tccaggtgat
    11101 gccgcgcctt ctccatgtcg cccagggcga gatagacctt gaccagcccc aggtggacct
    11161 cgggcagctc gctgtcctgg cgcagggcgc gggccatgag gtccagggcg gcggtggtgt
    11221 ccccccgccc cagcgccatc tccccctggt agacaaagaa gaacgggttg gaggagttgc
    11281 cctcctcgat gcgcgcggcg aggctcgccg cctcgtcggt ccgcccgagc tggtggtaga
    11341 gccgcaccag gttggtgagg agggtcgggt tgtccggggc ggcttcgagg ccgcggcgat
    11401 agaggtcgag cgcccgctcc ggctcgccgc cgcgggcgag gagcacccca aggttgttga
    11461 gcgccttttc gaagcgtggg gcgatctcgg tggcggtggc cagcaggcgg cgggcggact
    11521 cggtgtcgcc ggccagcagc gcctcggccc ccaggttgtt gaagaagtgg gccgccgccg
    11581 tcaggtcgtc gatcggcttg aaccccttgt agcccttggg acggtagggt aggaaatcgt
    11641 aggtctcgag ctgcccttcg aggtacatgc cggcgacgat atgaccctgg ctgaccacca
    11701 tcccctcgcg gctgctccac ttctggtagt cggtgacctc gacgtagaac ggggcgagac
    11761 cctgctcgcg ggcgatgccg acgaagaggt tgacgaacga gaggcagttg ccgcggcggg
    11821 tacggaacgt ctccgccgcg ttgcgggtcg gagcgagctc gtactggagg tcgaggcggt
    11881 cgaagatgaa gtcgatgacc tgcttgaggt tgcggcgctc gccggaggtc cggcgcagcc
    11941 ggccctgcag ctcggcgcgc agctcggggg agacctcgaa cggcacctcg agggccgcgg
    12001 cggcctgcgg gccgatccgc tgcgtgtact cgccgcgcag gtaggccgcg gagtcgaagc
    12061 gcttgtactc ggagcagccc gctgtcagga ccgcggaggc cagcaggacc gacgagagca
    12121 gcagacccgc cttccggaac atggagcacc aactatagca ggggcacgct caagagtccg
    12181 ggtcgggatc gagttcaaac atttgaagga gcaatgttca agccgttgaa tacgggcgcc
    12241 cgccggcgcc ccaccgaatg tggtaagtcg ctcatgatca aggagttaag acctgccggc
    12301 aggcgcctgc tttccgggca ccccgcttgc ttgatcccag ttggcgatgt cggctacagt
    12361 tcccgccaga aatattcccg ctgtggaagg aggtgtttgc aggcaccggt acctacccat
    12421 cgacgtcacg cgtccgagcc ggcgcgagtc caaaggctcg agttgtcgat gatcaactac
    12481 ccaagggagg ttcattccac atgaagagca ccaatttctg ggggcgggcc ctagccgtcg
    12541 ccgcggtgct gctcctggtg gccggcggct ccgccttcgc ccagttgcag accggcgcac
    12601 ttttcggcac cgccattgac gaccagggca acgtgttgcc cggcgtcacc gtcacgttga
    12661 ccggcgcggc gggaccgccg caggtccagg tcacggatgc gcaggggcgc ttccgttatc
    12721 ccaccctcgg ccccggctcc tacagcgtca aggccgagct cgagggcttc tcgcccgtcg
    12781 actaccccaa catctcgatc aacatcggcc gcaataccga gatcgagatc acgatgaact
    12841 cggcggtcaa agaggtgatc acggtcacca ccgagagccc gctgctcgac cagcgccgga
    12901 tctcggccgg cgcgacgatc gagcagaccg agctcgagaa gatcccgacc tcgcgcgacc
    12961 cgtgggcgat catgcagtcg accccgggcg tgctcaccga ccgcatcaac gtcggtggca
    13021 acgagagcgg ccagcagtcg cagtacgtgg gccccggctc cagcggtgac caggccgtct
    13081 ggtcggtcga cggcgtgacc atcaccgaca tgggcgccct cggctcctcg cccgcctact
    13141 acgacttcga ctccttcgag gagatccaga tcaccaccgg cggcagcgac accaccgtcg
    13201 ccaccggcgg catcgtcctc aacatggtga ccaagcgcgg caccaacgag tggcgcggct
    13261 ccggccgcta ctacgtcgcc gacaacgact ggcagtcgga cctcgacttc gacgagaacg
    13321 acctcgccca ggccggcccg tggagcaacg atacccccca ggaggctttc aaccagggca
    13381 accggatcgt caaggtcgag gactatggcg tcgagctcgg cggcccggtg gtcaaggacc
    13441 gtttctgggt ctggggctcc ttcggcaagc aggaggtggg cctgctgacg atcgacgacg
    13501 tcagcgactt caccgagctc gagacgatga acgtcaagct caacgcccag ctctcgcaga
    13561 gcaactcggc cacggcctcg ggcctgcaga gcgacaagat caagatcggc cgcaacgcgt
    13621 cgccgacccg gccgcagccg accacctgga accagagcaa gttcggtgac gacccgacga
    13681 cctggaaggt cgaggacacc catatcttca gcccgagctt ctacctgacc ggtatgtact
    13741 cggtggtcaa cggcggcttc caactcgtcc cccagggcgg catggacatc aacgccacct
    13801 gggacgagga cttcgtctgg cagaacagct tcctgctgta ccagaccccg cggccgcagg
    13861 agcagttcaa ggccgacgcc tcgaccttcg tcaacaccgg cagcgtggcg cacgagctca
    13921 agtacggcgc cggctaccgc gaggccgacg tcagctcgct ctcccgctgg cccggcttcg
    13981 gcatccagct aaacttctac cagtcgctcg gcaacgagga tccggccgac gacgtcccct
    14041 acaacgtcat cgagctgacg cgcgacgcgc tgccctcgtc gcacctcgag tacaccaact
    14101 tctacctcca ggacacgctg agcgccggca acctgacggc caacatcggc ctgcgctacg
    14161 accggcagga gccgaccctc gaccgggtca ccgtgcgcgc caacccggcc ttcccggatc
    14221 tccttccgga agccaccttc gagggtggca gtggaggctt ctcctgggaa tcgatcacgc
    14281 cgcgcctcgg cctgacctac gctctgggtc aggaacgcaa gacgctgctg cgggccagct
    14341 actcgcagtt cgccgaccag ctcggcaccg gcatcggcac ctggatcagc ccgctctacc
    14401 ccggcaccta cctctacctc tactatgacg accagaacgg cgacggcatc gcccagcagg
    14461 gcgaggtcct cgccaacacg ccgtgcaacg cctgcagcgt cccctacaac ggcacctaca
    14521 acccgctgac ccgcggcctg ctggtctcga acggcgtcga cccggacctc aagccgccga
    14581 tgacccacga gctgctgttc ggcatcgacc acgccgtccg gccggagttc ctgatcgagt
    14641 tcaaggcctc ctaccggctg ctgaccgacc tgctggagca ggagcgcctg gtcttcgacg
    14701 gtgacatcga ctacaacagc atcggccggc tgcaccgcgc cgatgactac gtgccggtga
    14761 ccaccaccac caccctgccc gacggccggg tccaggagtc cacctactac gtcctccggg
    14821 acggcgtcac cacccgcaac ggcttctggc tggagaacgg tgaccgcgag cagaacttct
    14881 tcggtgcctc gctgggcttc aacaagcgcc tggcgaaccg ctggatgctg cgcggcaacg
    14941 tgagctggca ggactgggag tgggacgtgc cgaacagcga gcgtgaagac cccacccgcc
    15001 tgctgggcgg cggcccgctg ggctccggct cgcaggacgg cgacccggtg ctccagggct
    15061 ccggctccgg ctccggcgcc aagggcggcg tctacatcaa cagcaactgg tcctacagcc
    15121 tcaccggcat gtaccagatc gcccccgagc gcccgtgggg cttcaatgcc gccctgagcg
    15181 cctacggccg cgagggctac ccgaacccct atttccgtcg ggtggaccgc ggcagcatca
    15241 gcgacttcct ggcccgcaac acctccatcc tggtggcgcc ggatcaggac agctaccgtc
    15301 tcgacgacat ccacatgatg gacgcgcgcc tggagaagga gttcacggtc agcgacctcg
    15361 gcctgacgct cacggccgac gtcttcaacg tgctcaacca gtcgtacgtc atccagcgcg
    15421 cccaccggct gaaccgggcc aatggcgatt tcgtccgcga gatcaccagc ccccgcatct
    15481 tccgtctggg tctgcggatc agcttccggt aagcgtgtaa gtccttctgc cgtaacgcat
    15541 ggagccgccg ggggaaaccc cggcggctct tttttgttgt agactctgcc gccggccggt
    15601 acgggctctc ccctcttttt cctctcagga ccccgtcgac cgaggccggc cgaaaggagc
    15661 gcttcctcat ggtcctgaaa atgctgcgcc gccgccgtct gccgctcgcc gccccctccg
    15721 cggtcatcgt ggccatcgcg gccttcgtgc tgtccctcgc cgcgccgggc acggcccagg
    15781 agtggaaggg gaagggacgc ctccaggggg atgtcctcga cgccgagggc aagccggtcg
    15841 cgggggccaa ggtcaccctc cgtccgggcg ccccgccggt cgacccggcc gccggcggac
    15901 cggcgccgat caccaccgac aagcgcggca agtgggcgat cctcggcctc gacaacggcc
    15961 catgggcggt gctgatcgag aaggagggct tcgtgcgctc cgaggggcag gtccaggtca
    16021 acgagtacgc cccggcgaag ccgatccgca tcgcgctcaa cccggccgcg caggagacgc
    16081 ggcaggcggc ggcggccaac gccgcgggcg aggaggcgct cgccgcgctc gaggccggca
    16141 acgccaagct ccaggccggc cagttcgccg aggcgcgggc cgagtacgag aaggccatgg
    16201 ccaccctcga gccggccaac cacccggcgg tcctgcgcgg catcgcccgc acctattacc
    16261 aggagaagaa gctcgaccag gcgatcgcgt cgctgcagaa ggcgctggag ctcaagccgg
    16321 acgacgccga gtcgcgcaag ctggtggcca acatctacgt cgacaagggc atcgaggcct
    16381 acaacgccac ccaggtcccg gaagccgccc agcagttcag cctggcgatc gagaacgacg
    16441 ggacgctcgc cgacgcctac tactaccgcg ggctggcgct gatggcccag accaagaacg
    16501 ccgaggccaa ggccgacttc cagaagctgc tggagctcga tcccaactat ccgcaggccg
    16561 cggacgtgcg ggagttcctc cagtcgctgt agcctccccg gccggcgcag gatccgtccc
    16621 atgcggcacc ccctgatgcc ggcgctcgcc ctcgccgccg ccttgatggc ggcggggtcg
    16681 ctcccgggct gcgcgcccgc cgatgaccgc gggaccgcgg aggccgcgcc ggcttcgtcg
    16741 gcgccgctcc ccgcctcggc ccgcggcgcc gacgtcatcc tgatcaccct cgacacgctg
    16801 cgggccgaca ccctggggtt cgccggccat ccgcgcgccg cgacgccggt cctcgaccgc
    16861 ctggcggcgg cgggccgggt ctacaccgac gcccatgccc acaacgtggt gaccctgccg
    16921 tcccacgcca acatcctcac cgggcgttac ccctaccagc acggcatccg cgacaacagc
    16981 ggcttcaccc tgccggcctc ggtccccacc ctggggacga tgctgcgcca ggccggctac
    17041 cgcaccggcg ccttcgtcgc cgcctacccc ctggactcgc gcttcggcct cgaccagggg
    17101 ttcgacctct acgacgacga ctacccgcgg gggtccaacc cggccgactt cgtcgtcgcc
    17161 gagcggcggg gggaccaggt gatcgcgccc gccctccggt ggtggcggtc gcaacgcggg
    17221 caccggcgct tcctctgggt ccacctgtac gacccgcacg ccgcctacgc gccacccgag
    17281 ccgttcgcca gccgcttccg cgacaatccc tacctgggcg aggtggcggc caccgactcg
    17341 ttcctcgcgc cgctcctcca gccgctcctc ggaggcaagg agccccccgc cctggtggtg
    17401 gtcacctccg accatggcga agcgctcggc gagcatggcg agctgaccca cggcctgttc
    17461 gcctacgagg cgacgctcaa ggtgccgctg gtgctctggg gagccggcgt ccgtcccggc
    17521 aaggacaccc ggccggcgcg gcacatcgac atcgtgccga cggtgctcgg agcgctgggc
    17581 gtcaagtctc cctccggcct ccctggccgc tcgctcctcg ggccggcgtc ccagacggac
    17641 accagctact tcgagtcgct ctccaccgcg ctcaaccgcg gctgggcgcc gttgcgcggt
    17701 acgctccgcg agcgcaggaa gtacatcgag ctgccgctgc ccgagctcta cgacctcgcc
    17761 cgtgaccccg gcgagcagaa gaacctcttc ggcgaggagc gccgcgccgc caacgccctg
    17821 cgccaggcgc tgccggagga gtcggtctgg ccgccgcgca aggggcaggt gtcgcccgag
    17881 gaggaggcgc gcctgcgcag cctcggctac atgacgggtg acgccggtga caaggccagc
    17941 ttcgccccgg cggacgaccc caagaacctg gtcgccctgg accgccagct ccaccaagtg
    18001 gtggacgcct actcgcgcgg ccgctacgag gaggcggcgc gggtggcccg gcaggtcgtg
    18061 gcggcccgcc ccaccctcgc cgagggctac gagcacctgg cactggcgct gcgccagctc
    18121 gagcgccagg acgaggcggt caccgtgctg cgccaggggg tgacccgcat cgggcagggc
    18181 ggcggccggg actcgctgcg ccgccagctc ggccaggcgc tcgccgaggc gggccggccc
    18241 gccgaagcgg tcgaggtgct ggcgccggtc gcctccggca gctccgacgc ctcggtcctc
    18301 ggcgcctacg gcatcgccct ctccgacgcc ggacggcacg gcgaggccgc cgcggtcctc
    18361 cagcgcgccc tgtcggccga tccgtccaac cccaaggtgc tggagaacct cggcatcatc
    18421 gcgttgcgga tgcggcagcc ggcggcggcg cgcgaccacc tccgccaggc gctcgccctg
    18481 aacgaccggc tgcccatcgc ctggaacacc ctcggcgtcg ccctctacca gctcgagggg
    18541 ccgccggccg cggccgccgc actcgacgcc tgggagcggg cggtggccat cgacccggcg
    18601 cagtacgacg ccctctacaa catcggcctg gtggcccgtg aggcggggcg gcgggagcag
    18661 gcgcggcgcg ccctcgagcg cttcgtccgc accgcgccgc cgtcccgctt cggccccgac
    18721 ctggccaagg cccggcagat cctcgccggc ctgggagcat gagcatgatc cgacgcctcc
    18781 ccctcctctc cctcgtcctc ctgctggcgg gagccgccgc ctgctccgac gccgggggcg
    18841 gcggcggccg gggcggcggc gaggtgttcg aagacgcgcc ggtgatcctg atctccatcg
    18901 acaccctgcg ctcggaccgg ctgccggcct acggctacga gaaggtggag acgccggcga
    18961 tcgacgccct ggccaaggac tccatcctct atgaacgggc ctacagccac tatccgctga
    19021 ccctcccttc ccacgtctcg atgatgtcgg gcctgctgcc cccgcggcac ggcgtgcgcg
    19081 acaacgtcgg ctactcgttc gacgccgaga agcacccctc cctcgcccgg ctgttcaagg
    19141 aggccggcta cgagaccatg gccgcggtct cggccttcgt gctgcgcggc tcgaccggca
    19201 tgggggaggg gttcgacacc tatgacgacc ggctgatccc caaggagggg cagagcctgg
    19261 acgccgtgca gcgcgccggc gacgaggcca cgcggtcggc cacggcgtgg gtgcgcgagc
    19321 gcgccggccg cccgttcttc ctcttcctcc acctgtacga gccgcacgcg ccctacatgc
    19381 cgccggagcc gttcaagtcg cgcttcgccg acccgtacga cggcgaggtg gcggcggccg
    19441 acgcctacgt cggcgagctg ctggcggagc tcaagaagct ggagatctac gacaaggcgg
    19501 tcatcgtgct cctctccgac cacggcgagg cgctgggcga ccacggcggc cagacccacg
    19561 gcgtcttcct ctaccgtgag gcgctccagg tgccgctgct ggtgaagctc cccggcgccc
    19621 gccatggcgg cacgcgggtc gcggcgccgg cgcggctggt ggacgtcttc ccgacgctgc
    19681 tcgccaccgc cggcctcgac gtcccgcagg gggacggggc gtcgctgctg gagctcctgt
    19741 cccccggcgc gccgccgcgg caggtctact ccgagacctt ctacccgcgc atccacttcg
    19801 gctggagcga cctcgcctcg gtgatcgacg gcccgcacca ctacatcgag gggccggacc
    19861 ccgagctgta cgacctcgtg aaggacccca gggagctgga gaaccgcttc ggctccgacc
    19921 ggcgggcggt ggggaccctg cgccaggcgc tggcgaaggt cgaccgcacc ttcaacccgc
    19981 cgggcgaggt ggacgcggag accgcccgca agctcgccgc cctgggctat gccgcgggcg
    20041 gcggaccggt gccggagggg ccgctccccg accccaagag ccagctccac gtggtccaga
    20101 agatggaggc ctcgttcagc gccttcgatc acggcgatta tgagaaggcg gcgcaggggt
    20161 tccgggagct ggtcaccgag aacgc
//
LOCUS       DASPJI010000431         3229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_141688, whole genome shotgun sequence.
ACCESSION   DASPJI010000431 DASPJI010000000
VERSION     DASPJI010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141688"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VEL74_11650"
     CDS             complement(<1..211)
                     /locus_tag="VEL74_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63227.1"
                     /translation="MAAGDRQDSWTGRTISHYRLLERLGSGGMGVVYKARDLLLERLV
                     ALKFLHAGRHDDVSRQRLLHEARSVS"
     gene            complement(245..2701)
                     /locus_tag="VEL74_11655"
     CDS             complement(245..2701)
                     /locus_tag="VEL74_11655"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63228.1"
                     /translation="MTSQRPGLPPEIEPVMARLLARDRAARYQRADEAARDLLALQVD
                     LPVHPADRPVDRPEEDGTAAPTLVLEPGWRPAVKRAIEVHPRVSDVFVGRQEELTALR
                     QALLTEPAEGLPATVCAIQGMPGVGKSYLADRFAVDHMDRFPGGCLRLVVDPKAPAAP
                     RAMLDELAARLELAGGDGVAARVRERLLQPRTLLHIENVDSPAAEVEAGRLLRELPGC
                     AVLVTGRLHDLGFAMGWRQVRMTAFDEETALLQLRGELGWTPSPEDEAGYRELVRSLG
                     HLPLAVHLAAGHLRSGRSATGFLRMLRQRRLAITPADRTELAVGIAGEARNVLATSFS
                     ISLEILREEIGPEAGRLLAGLHALGHAPLTGFGRGLGAALAGLPDDDFEELVFHAQKL
                     CQLLPIPRQERPDGAWRLHPLLAELLAAEASGEAAMDRMTRWFAARLPLGAPGEEAEQ
                     GRRWREVRAESAALAWWLPRVPAGDRWTVQQAGTWYARFNGPFHAWSEFCEEMLQNFD
                     DPRQRGEILWTLGQVSLRQGRLDRAMEAAEEKLWLDRRRGDRFEEATAWELLADVLQA
                     RGELDDALATFQERQLPILEELGDSRSRAICLAKVGYLLKLRGCFGQALEVLREAQSA
                     LEEAGDVRSRAVALGFIADTLEAQGDRDESLRIRQLEELPIYQVLGELRATAVTMGKI
                     AGILHARGEHETALAIRQEQEKPIYESLGDARQLSLCLGRIADIRRGLGEIDEAIRVR
                     QAEQLPLLERLGSGYDLLFAREGLALLLLERRQPGDLEQAAALLRQALDAAGRLRLPE
                     ADRIRALLDEHGLVTRTVYC"
     gene            complement(2698..>3229)
                     /locus_tag="VEL74_11660"
     CDS             complement(2698..>3229)
                     /locus_tag="VEL74_11660"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYG63229.1"
                     /translation="LLEARAASALDHPNVCAVYEIEETPDGYLFFAMGFCDGESLRAR
                     IDRGAVEAGEALGLAAQIAAGLAHAHDRGVIHRDIKPANLILTPDGPLKRVKIVDFGV
                     ALRNGGSRLTRYGSTVGTVAYMSPEQLRGETVDHRADLWSLGVVLYELLTGALPSMPP
                     PRWPMPRRSSARSRGA"
BASE COUNT          435 a         1152 c         1232 g          410 t
ORIGIN      
        1 aggagacgga acgggcctcg tgcaggaggc gctggcggga gacgtcgtcg tgacggccgg
       61 cgtggaggaa cttgagggct accaggcgct ccaggagcag gtcccgggct ttgtagacca
      121 cgcccatgcc accactgccg agcctctcca gcagccggta gtgggagatg gtacgtccgg
      181 tccaggaatc ctggcggtcg ccggcggcca tgagctcccc tgagcaagcg ttgggacgcg
      241 catcttagca ataaacggtt cgggtgacga ggccgtgctc gtcgaggagc gcgcggatgc
      301 ggtccgcctc gggaaggcgg aggcgcccgg cggcgtccag cgcctggcgg aggagggcgg
      361 cggcctgttc caggtctccc ggctgccggc gctccaggag gagcagcgcc agaccttccc
      421 gggcgaagag caggtcgtag ccggagccga gccgctccag cagggggagc tgctcggcct
      481 gccggacgcg gatggcctcg tcgatctccc ccaggccgcg gcggatgtcg gcgatccggc
      541 ccaggcagag ggagagctgg cgggcgtccc ccaggctctc gtagatcggc ttctcctgct
      601 cctggcggat ggcgagggcg gtctcgtgct ccccccgggc atggaggatg cccgcgatct
      661 tgcccatggt caccgcggtg gcccggagct cgccgagcac ctggtagatg ggcagctcct
      721 cgagctggcg gatgcgcagg gactcgtccc ggtccccctg ggcctcgagg gtgtcggcga
      781 tgaaacccag ggccacggcg cgggagcgca cgtccccggc ctcctcgagg gccgactggg
      841 cctcgcgcag cacctccagg gcctggccga agcagccgcg cagcttcaga aggtagccga
      901 ccttggccag gcagatggcg cgggagcggg agtcgcccag ctcctccagg atcgggagct
      961 gccgctcctg aaaggtggcg agggcgtcgt cgagctcgcc gcgggcctgg agcacgtcag
     1021 ccaagagctc ccacgcggtc gcctcctcga aacggtcacc gcgccgcctg tcgagccaga
     1081 gcttctcttc ggccgcttcc atcgcgcgat cgaggcgtcc ctgccgcaga gagacctggc
     1141 cgagcgtcca gaggatctcg ccgcgctggc gcggatcgtc gaagttctgc agcatctcct
     1201 cacagaactc gctccaggcg tggaaggggc cgttgaacct ggcataccac gtccccgcct
     1261 gctgcaccgt ccagcggtcg ccggcgggga cccgtggcag ccaccaggcg agggcggcgc
     1321 tctcggcgcg gacctcccgc caccggcgcc cctgctcggc ctcttcgccg ggcgcgccca
     1381 gcggcaggcg ggcggcgaac caccgggtca tccggtccat ggccgcctcg ccgctggctt
     1441 cggcggccag caattcggcc agcagcgggt ggagccgcca ggcgccgtcg ggtctctcct
     1501 gccgggggat ggggagcaac tggcagagct tctgggcgtg gaagaccagc tcctcgaagt
     1561 cgtcgtccgg caggccggcg agggcggcgc cgaggccgcg gccgaaaccg gtgagcggcg
     1621 cgtgtcccag ggcgtggagg ccggcgagca gccgccccgc ctccgggccg atctcttcgc
     1681 gcaggatctc cagcgagatc gagaagctgg tggcgaggac gtttcgggcc tcgcccgcga
     1741 tccccaccgc cagctcggtc cggtccgccg gcgtgatcgc caggcggcgc tgccgcagca
     1801 tgcgcaggaa gccggtggcc gagcgccccg accgcaggtg ccccgccgcc aggtggacgg
     1861 cgagaggcag gtggcccagg ctccgcacca gctcccggta gccggcttcg tcctcggggg
     1921 acggcgtcca gccgagctcc ccccgcagct ggaggagggc ggtctcctcg tcgaaggcgg
     1981 tcatccgcac ctgccgccag cccatggcga agcccagatc gtggagccgt ccggtgacga
     2041 ggacggcgca acccggcagc tcccgcagca gccgccccgc ctccacctcg gcggccgggg
     2101 agtccacgtt ctcgatgtgg agcagggtcc gcggctggag cagccgctcc cgcacccgcg
     2161 ccgccacccc gtcgccgccc gccagctcga ggcgcgcggc gagctcgtcg agcatcgccc
     2221 ggggcgccgc cggcgccttg gggtcgacga cgagacggag gcagccgccg gggaaccggt
     2281 ccatgtggtc gacggcaaag cggtccgcca ggtaggactt gcccacgccg ggcatcccct
     2341 ggatggcgca gaccgtggcc ggcagcccct cggcgggctc ggtgagcagg gcctggcgga
     2401 gcgccgtgag ctcttcctgc cgcccgacga agacgtcgga cacccgcggg tggacctcga
     2461 tcgcccgctt caccgccggc cgccagccgg gctcgaggac cagggtgggg gccgcggtac
     2521 cgtcctcctc cggccggtcc accggccggt ccgccgggtg gacggggagg tctacctgca
     2581 gggcgaggag gtcccgggcg gcctcgtcgg cccgctggta gcgcgcggcc cggtcgcgcg
     2641 ccaggaggcg cgccatgacc ggctcgatct ccggcggcag gccgggccgc tgggaggtca
     2701 cgcgccgcgg ctccgtgccg aggaccgccg cggcataggc cacctcggtg gcggcatcga
     2761 gggcagggcg ccggtcagca gctcgtagag cacgacgccc agggaccaga ggtcggcgcg
     2821 gtggtccacc gtctcgcccc ggagctgctc gggggacatg taggccacgg tgccgacggt
     2881 cgagccatag cgggtgagcc gggagccgcc gttccgcagg gcgacgccga agtcgacgat
     2941 cttgaccctc ttcaggggac cgtccggggt gaggatgagg ttggcgggct tgatgtcccg
     3001 gtggatcacg ccgcggtcgt gggcatgggc cagcccggcg gcgatctgcg ccgcgaggcc
     3061 gagcgcctcc ccggcctcga cggcgccgcg gtcgatccgc gcccgcaggg attccccgtc
     3121 gcagaacccc atggcgaaga agaggtagcc gtcgggcgtc tcctcgatct cgtagacggc
     3181 gcagacgttg gggtggtcca gggcggaggc ggcgcgggct tcgaggagc
//
LOCUS       DASPJI010000432        12794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_371954, whole genome shotgun sequence.
ACCESSION   DASPJI010000432 DASPJI010000000
VERSION     DASPJI010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12794)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12794
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371954"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VEL74_11665"
     CDS             <1..923
                     /locus_tag="VEL74_11665"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYG63230.1"
                     /translation="RLVIVADGALHYLPFAALPDPASGDPLLITHEVVHLPSASVLAI
                     QRRELQDRTPAPAALAVLADPVFSPSDPRVGGGRGPSGPAQATGSVVGEEGGAHTKEP
                     SRAGSGLAPEGVFDRLPATRREAESIAALASPAESLTALDFQASRQTALQGGLDRYRI
                     VHFATHGVIQAETPELSGLALSMVDEAGRPADGFLGLRDIYNLRLRAGLVVLSGCETA
                     LGREIRGEGLAGLARGFMYAGAARVVASLWRVEDRATAELMTRFYQGLLVDRLPPAAA
                     LRRAQLAIRAERRWRDAYHWAPFVLMGDWN"
     gene            926..1534
                     /locus_tag="VEL74_11670"
     CDS             926..1534
                     /locus_tag="VEL74_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG63231.1"
                     /translation="MATPETLLEPGEEEIAAGLVRRIGEGDTRAEGELVERYSRGLLY
                     MLRRRAGDPALADDLHQETFRIVLERLRRQGIEDPARLSGFILQTARNLFLGDYRKKT
                     RRGEGHDVEESPEPADPAPGQLTGVLRREEAARVRQVLAEMGTDRDRQILFRFYIAEE
                     DKEAICADLGLSSLHFNRVLFRARERFKSLMTRAGPAPERPG"
     gene            1592..2449
                     /locus_tag="VEL74_11675"
     CDS             1592..2449
                     /locus_tag="VEL74_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63232.1"
                     /translation="MDHSYIDEQQVAERYVLGQLSQEEADLFEEHYLHCTQCLDRLEA
                     AEGLQRGLERVAEEEVARAVVARAGVLAAVGRLLRSRQAPLAVTLLLLVALLPSGLLL
                     WRVNDLEQAVESRPVSSTPAPSPSPATPTPPLPPAVDLRPELEAVRGELAEERRRSEE
                     LAGELSRAREPQVNVPILTLAPERSAPGQEDEPTHRVSLAASPEWIVLALEVGGPDHP
                     FYRAVLTGPDRQPVWQASGLQADATGLITLSLPTSLLRAGDFAVRLEGMPAAASPVTV
                     ARFSFRVTP"
     gene            2525..2956
                     /locus_tag="VEL74_11680"
     CDS             2525..2956
                     /locus_tag="VEL74_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63233.1"
                     /translation="MSDQKSRPPLGLELWTLKEWTNHRQAPFDLKKDNFSVAWHPTDG
                     GYVQFRLVGGGDHEHEFKRHTDGCFRGELKIGKRQFEFSFVVTGTTPGRLMKGEFYEI
                     SKETGPDPVASWGAEERGGGPIIGHEGHGRGERRPEPPVRR"
     gene            3174..3572
                     /locus_tag="VEL74_11685"
     CDS             3174..3572
                     /locus_tag="VEL74_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63234.1"
                     /translation="MSEQHNDRERELKNPATVQQELLAAGNGGGQMEKSAVQAGNVQV
                     CLGAENGSFKLHWAGPTFRSDDFVALFANDGSPDDQWLLNQWQWAVRGTSYVTGTSVN
                     PSYQARYMTWMNNRWTSIARSPAFPDKVCG"
     gene            complement(3631..4572)
                     /locus_tag="VEL74_11690"
     CDS             complement(3631..4572)
                     /locus_tag="VEL74_11690"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYG63235.1"
                     /translation="MKLAVAFLALLTGCATTTAVQPPAPAVDYHQHLVSAAFAPIVKF
                     PERDGAALVRELDAAGIDRAVVLSVGYSFADERKGLSDPDRLTREENDWTSAEVVKHR
                     RRLIGFCSANPMREVALQELERCLGLPGMTGIKVHMGNGGVTLRNPAHLARIQELFAL
                     AQRLGAPVLVHMRARGGESYGAEDARLFLDQVMPKAPGIEVVVAHLGASGPGYPAQND
                     EVMAVFGEAAQRRDPRLRNVYFDVATNVTAEATPADGALVAQRVRQVGVERVLYGSDL
                     NPPGGGIRQGWEIFRTKVPLTPAELQQIAGNRTRFAR"
     gene            4763..5164
                     /locus_tag="VEL74_11695"
     CDS             4763..5164
                     /locus_tag="VEL74_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63236.1"
                     /translation="MRAHHRSLRTRGFGNESAQIGDVPPETAVNPFGMDTVLRGTASI
                     PEETVFISSETAFISEGTPALSLEMRSIPGEMAILPEEMETIPGGMAAISWETESVSA
                     GMTMISVEATMGCCAPPVPLEEIFSRFFPED"
     gene            5373..5924
                     /locus_tag="VEL74_11700"
     CDS             5373..5924
                     /locus_tag="VEL74_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63237.1"
                     /translation="MRNYLDGSTGDRVPSSTPNTAPGTSGGPENAFTPEYLDLLNQRD
                     EPPTASDADAAGPWHLEPAPDGRWAVLRVGEALGRGDVPAGDFQMKQAAQLAAALSPG
                     TGKSKRYRQGKEDDGRGYPVYYDGELAGHLRVFDEDLIASMNAADALLSAPYDFAWLL
                     DAAGGLALERTGKVSLARARGDL"
     gene            complement(6152..7744)
                     /locus_tag="VEL74_11705"
     CDS             complement(6152..7744)
                     /locus_tag="VEL74_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HYG63238.1"
                     /translation="MERLRQEEERIRLGGGAKAQDKQRAQGKMTARERVAALCDPGAP
                     FLELGLWVADGFYKEYGGAPSAGVVIGIGAIHGRDVVVVANDATVKAGAWFPLTCKKV
                     LRAQEIALENRLPIVYLVDSAGVFLPMQDEIFPDKEHFGRAFYNNARLSAEGIFQMAA
                     IMGPCVAGGAYLPIMSDESHIVEGTGSIFLAGSHLVQAAIGEKIDNEELGGALVQCDV
                     SGVVDHRHPDDASCLAKIRSQFAQMAAGEMAPFARRAPADPLYPSEDLYGLVPIDRSR
                     PYDTHEILARLLDGSEFDEYKATHGQTLVCGTGWIGGWAVGIVANQRSIVPRRTGTGP
                     ADRELQIGGVIYSDSAAKGARFVELCNQKKIPLLFLQDVTGFMVGSRAERGGIIKDGA
                     KMVNAVANSTVPKLTLFVGNSFGAGNYAMCGKAYGARFLYAWPSATISVMGGDQASKT
                     LLSIQLKNRGEDVSEEEKKQRLSEIKARYDAASNPRYAAARLWVDALIDPAETRDILA
                     RSLACAAMNPHLAEFKTGVLQT"
     gene            7965..9017
                     /locus_tag="VEL74_11710"
     CDS             7965..9017
                     /locus_tag="VEL74_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63239.1"
                     /translation="MRPFLVLGVLLLCLTVAAPALAVTGTADHVPAATLLVPFFETGI
                     NVTTHPHDTLMVVTNWLFADVTFHYHVWDVDGNATGLSGNILLDDLDTWSAAMRDLLN
                     ASSPAVRAQLTRGAFYRGFVTIDVVTAATALNPRQAGYPFGTTNALEGFTYYTRLSQG
                     SANGLAMVPLEHVPATTDSFMRGFYSAGDNREEIDSTGRFCANQLASNPNGTTGCNTA
                     GDDSDIDRFHLRIFRSAPLTGNSRAVIFTWRPGLVGGPSIFCDTQPCESAYVFRQYDE
                     AGTAVQSSTIRLNHVVNVIENSQLVGTQAGWLSIFDVPNNQVDTHVYAFSFNSAAPAG
                     NPNLTWDAIFEGYIVP"
     gene            complement(9044..12142)
                     /locus_tag="VEL74_11715"
     CDS             complement(9044..12142)
                     /locus_tag="VEL74_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63240.1"
                     /translation="MGTRRALWLLVVPLLAGAVTMAVGAPAAESEVGTDADPAALCSA
                     PLPLPSRFPADKSAYERLLGRFLAARCYLELGWKHDKQVRPTGPTIAALGGDPHAPAW
                     SPTTWGTHNTVAIYYSPDVYRWMCERDAREERRFVDQCRTTCPDCRLDDRAPVRPIAD
                     GSMILKLMYGNMTAARLEDPSIPDGPPNMVALMVRDRRGSRDGWFWGSWDPQATKASQ
                     LDWPPPVNLPYPWMGFGYYCVNCHASAQDEFTFSSLNNVLGDPDTFLKYYAQDQPPIV
                     GAAPRPAATPVETLSSHERFDPVRDMDAEAGETPWNQVPWPNPEVQRVSQPRASYSPA
                     FLATFGARSIQPPDGRQITRLYMPPEVYDSVPPPADKPALFLTSDQCLGCHAAGSTGM
                     HFDMTLQQAGQPAYQKLLNLAPYGEWRGSPMGLAGRDPIFFSQLESEETLHPKLGKLV
                     QDLCLHCHGVMGQRQNCLDQFPGDPARANEVCDNTDLLGLDESSQPIVPRKLFSRAAL
                     NTVPYGAATPEQQQEARYGALARDGVSCTTCHHIRVDQTTPIGHTFTGDFRVGAPEEI
                     HGPFEGPQQVPMEHTLGAKPMAYPLIRSSKVCGSCHSVVLPVFDGDQPWVRPGESKPE
                     IIIEQATYPEWVFSDFRDDGATPRSCQDCHMASSYPGVTGPLSFKIASIQEASNMPQT
                     ENRRPESEIDLKPRSPFGRHTLVGLNVFFNLFAQQFPDILGIRIQDPMLGGRGVAPLQ
                     TTYDSMIQQADTATAKVSVTGVRRTASDLVAEVRVENLAGHKLPSGVGFRRAFLTFEV
                     LDGSGKPLWVSGRSTSTGVLVDEAGKPLPGEHFWKSDCRPMTVAEQRDHFQPHYEVIT
                     RQDQAQIYQELVRDPRGRFTTSFLSLADEVKDNRLLPRGWEPSLELALREGLGSPKLP
                     AEDLVERVLPDLPDGRGGHVRDPWYEPKSRGGLGGGGDALTYAVPLNALRGRKPAGVR
                     VTLYYQSIPPFYLQDRFCTTPQRPDTQRLFFLAGHVDLDGTRAEGWKLQVVSSGVVGV
                     DG"
     gene            12282..12527
                     /locus_tag="VEL74_11720"
     CDS             12282..12527
                     /locus_tag="VEL74_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HYG63241.1"
                     /translation="MEDVFEGRWKQLKGDAKRAWGKLTDDDWDRAEGNRDKLVGSLQE
                     RYGWEKVHAESEFDRFLESIRTPAGAGAGTDRTGDRY"
     gene            12606..>12794
                     /locus_tag="VEL74_11725"
     CDS             12606..>12794
                     /locus_tag="VEL74_11725"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HYG63242.1"
                     /translation="MKIRLLAFATAADALGTDETEMELPDGSRVSDLRARLDREHPSL
                     SPLWPRLAVAVDGRVVPPD"
BASE COUNT         1932 a         4431 c         4504 g         1927 t
ORIGIN      
        1 cgcgcctggt catcgtcgcc gacggagcgc tccactatct ccccttcgcc gccctgcccg
       61 acccggcctc cggcgacccc ctgctcatca cccacgaggt cgtccatctc ccctcggcct
      121 cggtcctcgc catccagcgc cgcgagctgc aggaccgcac accggccccg gccgccctcg
      181 ccgtcctggc tgaccccgtt ttcagcccca gtgacccccg ggtgggggga ggccgaggcc
      241 cgtccggccc ggcgcaggcg actggcagcg tggtcgggga ggagggcgga gcgcacacga
      301 aggagccgag cagggccgga tcgggcctcg cgccggaggg ggtcttcgac cgcctgcccg
      361 ccacccgccg cgaggcggag tccatcgccg ccctcgcttc gcccgcggag tccctgaccg
      421 ccctggactt ccaggccagc cgccagaccg cccttcaggg cggcctcgac cgctaccgta
      481 tcgtccactt cgccacccac ggcgtgatcc aggccgagac gccggagctc tccggcctcg
      541 cgctctccat ggtggacgag gccggccggc ccgcggacgg cttcctgggg ctgcgcgaca
      601 tctacaatct ccgcctccgc gcgggcctgg tggtgctctc cggctgcgag acggcgctgg
      661 gccgggagat ccgcggcgag gggctcgcgg gcctggcccg cggcttcatg tacgccggcg
      721 cggcgcgggt ggtggcgagc ctctggcgcg tcgaagaccg ggccaccgcg gagctgatga
      781 cacgcttcta tcaaggtctc ctcgtcgacc ggctcccgcc ggccgcggcc ctgcgccggg
      841 cgcagctcgc catccgcgcc gagcgccgct ggcgcgatgc ctatcactgg gcccccttcg
      901 tcctcatggg ggactggaac tgacgatggc tacccccgag accctcctgg agcccggcga
      961 agaggagatc gcggccggcc tggtccgccg gatcggcgag ggcgacaccc gcgccgaggg
     1021 agagctggtc gagcgctaca gccgcgggct cctctacatg ctgcggcgcc gcgccggcga
     1081 tccggcgctg gcggacgacc tccaccagga gaccttccgt atcgtcctcg aaaggctgcg
     1141 ccgccagggg atcgaggacc ccgcccggct ctccggcttc atcctccaga cggcgcgcaa
     1201 cctcttcctg ggcgattacc gcaagaagac ccgccgcggc gaggggcacg acgtggagga
     1261 gtcccccgag ccggcggacc cggcgccggg ccagctcacc ggcgtgctgc gccgcgagga
     1321 ggcggcgcgg gtgcgccagg tcctcgccga gatggggacc gaccgcgacc gccagatcct
     1381 gttccgcttc tacatcgcgg aggaggacaa ggaggcgatc tgcgccgacc tggggctgtc
     1441 gagcctccac ttcaaccgcg tcctgttccg tgcgcgggag cgtttcaagt ccctgatgac
     1501 gcgcgccggc cccgcgccgg agcggccggg gtgagatggc gaggcgggcg gcgacagtcc
     1561 catcatgagg aggaagggcc gaggtcaagg gatggatcat tcatacatcg acgagcagca
     1621 ggtggccgag cgctacgtcc tgggccagct ctcccaggag gaggccgacc tcttcgagga
     1681 gcactacctc cactgcacgc agtgcctgga ccgcctggag gcggcggaag ggctgcagcg
     1741 gggattggaa cgggtggccg aggaggaggt ggcgcgagcc gtggtcgccc gcgccggcgt
     1801 cctcgccgcc gtcggccggc tcctgcgctc gcgccaggcg ccgctggccg tcaccctgct
     1861 gctcctggtg gcgctcctcc cctcggggct actcctctgg cgcgtcaacg acctcgagca
     1921 ggccgtggag agccgtcccg tctcctccac gccggccccc tctccctctc ccgccacccc
     1981 cactccgccc ctgcctcccg cggtggatct ccggccggag ctggaggcgg tccgcggcga
     2041 gctggccgag gagcggcgcc ggagcgaaga gctggccggc gagctctccc gtgcccgcga
     2101 gccgcaggtc aacgtgccga tcctcaccct cgccccggaa cgctcggcgc cgggccaaga
     2161 ggacgagccg acccaccgcg tctccctcgc cgcctcgccg gagtggatcg tcctcgccct
     2221 ggaggtgggc ggtcccgacc acccgttcta tcgcgccgtc ctgacggggc cggaccgcca
     2281 gcccgtgtgg caggcgagcg gcctgcaggc ggacgctacg ggcctgatca ccttgagcct
     2341 tcccacgagc ctgctccggg ccggcgactt cgccgtccgc ctcgaaggga tgcccgcggc
     2401 ggcatccccc gtcacggtcg cgcgcttctc gttccgggtg acgccatagg agccgggtga
     2461 gatagcgggc gcgctcgcgg cagtccaaat tcgagaacga cacgatttcc aacaaggagg
     2521 cgacatgagc gatcagaaga gtaggccgcc cctcggtctc gagctctgga ccttgaagga
     2581 gtggacgaat caccggcagg cgccgttcga tctgaagaag gacaacttct ccgtcgcctg
     2641 gcaccccacc gacggcggtt acgtgcagtt ccgtctggtg ggtggtggcg accacgagca
     2701 cgaattcaag cgccacacgg acggatgctt tcggggcgag ctcaagatcg ggaagcggca
     2761 gttcgagttc agcttcgtcg tcaccgggac gactcccggc cggttgatga agggcgagtt
     2821 ctacgagatc agcaaggaga ccggtccgga cccggtggcg agctggggcg ccgaggagag
     2881 gggcggcggc ccgatcatcg gccacgaagg gcatgggcgc ggcgagcgcc ggcccgaacc
     2941 ccccgtgcgg cgctgagccg cgctttccat ccttccctct ctcttccctt tccatccact
     3001 ctccctcctc gccgcgggca cgcggggggg agggattccg ctttccgaac cggtcgcgaa
     3061 attcgttcga agaagggtga gatagccagc ttcctggtga cagtccatct atgaaagcga
     3121 cgcgcgcgtt ctgatcacaa ccacgaaacc aaacgattcg aaaggagatc accatgtccg
     3181 agcagcacaa cgaccgcgag agagagctca agaaccccgc caccgttcag caggagcttc
     3241 tggccgccgg caacggcggc ggccagatgg agaagagcgc cgtgcaggcc ggcaacgtcc
     3301 aggtctgcct gggagccgag aacggctcgt tcaagctgca ctgggcgggc cccaccttca
     3361 ggagcgacga cttcgtggcc ctgttcgcga acgatggctc gcccgacgac cagtggctcc
     3421 tcaaccagtg gcagtgggcc gtccgcggca cctcgtatgt gaccggcacc tcggtcaacc
     3481 ccagctacca ggcgcgctac atgacctgga tgaacaaccg ctggacgagc atcgcccgca
     3541 gccccgcctt ccccgacaag gtctgcggct agccctcttc ccttcacacc cgaggaggca
     3601 ggccggagac cggcctgcct cctggctcgt tcagcgggcg aaccgggtcc ggttgccggc
     3661 gatctgttgc agctcggccg gggtcagcgg caccttggtt cggaaaatct cccagccctg
     3721 gcggattcct ccccccggcg ggttgaggtc cgatccatag agcacgcgct ccacgccgac
     3781 ctggcggaca cgctgggcga cgagcgcgcc atcggcaggc gtcgcctcgg cggtcacgtt
     3841 cgtggcgacg tcgaagtaga cgttgcgaag acgcgggtcg cggcgctgcg ccgcctcgcc
     3901 gaacaccgcc atcacctcat cgttctgcgc cggatatccg gggccggagg cgccgaggtg
     3961 cgcgacgacg acctcgatgc ccggcgcctt cggcatcacc tggtcgagga acagccgcgc
     4021 gtcctcggcg ccgtagctct cgccgccgcg ggcgcgcatg tggacgagga ccggcgcgcc
     4081 gagccgctgg gcgagcgcga acagctcctg gatgcgggcg aggtgcgccg ggtttcgcag
     4141 ggtgacgccg ccattgccca tgtggacctt gatgccggtc atgcccggca gacccaggca
     4201 gcgctccagc tcctgcagtg ccacttcgcg catcggattg gccgagcaga agccgatcag
     4261 ccgccggcgg tgcttgacga cctcggccga cgtccagtcg ttctcctccc gggtcagccg
     4321 gtcgggatcg ctcaacccct tgcgctcgtc ggcgaagctg tagccgaccg acagcacgac
     4381 cgcgcggtcg atgccggccg cgtcgagctc acgcaccagc gccgcgccgt cccgttcggg
     4441 gaacttgacg atcggagcga acgcggcgct gaccagatgc tggtggtaat cgacagccgg
     4501 ggcggggggc tggacggcgg tggtcgtcgc gcagcccgtg agcagcgcca ggaaggcaac
     4561 ggcgagtttc atgggggcta cgctacccag gtcgcgcgat ggaagcaagc gggtgcgccg
     4621 gccgtcccga ggggcccggt ggcgggatcg ttgggattgg gagagcggtg tgcgccaccg
     4681 cggaggaggg tggggagaag gtgagggcgg ggcgggcggt tcgcttctac gtctgcggcg
     4741 cttgcctctc gagttccaag acgtgcgagc tcatcaccgc tcgctccgta ctcgcggctt
     4801 cggcaacgaa tcggcacaga taggcgatgt tcctcccgaa accgccgtca acccttttgg
     4861 aatggacacc gtccttcggg ggacggcctc cattcctgaa gaaacggtct tcatttcctc
     4921 cgaaacggcc ttcatttctg aaggaacgcc cgccctttcc ctggaaatga ggagcattcc
     4981 tggagaaatg gccatccttc ctgaagaaat ggagacgatt cccggaggaa tggcggccat
     5041 ttcttgggaa acggagagcg tttcggcggg gatgacgatg atttccgtgg aagcgacgat
     5101 gggctgttgc gcgccgccgg tccccctgga ggaaatcttt tcgagattct tcccggagga
     5161 ttgacttccc tccgcctcct tcatacgcta gggaatgtca acccgccgca gtcgttcgtc
     5221 gccggggagg aggtgagacg gagagagacg aagcctgaag aatcccgctc gtgaagtgaa
     5281 gaggccggcg tgtgtccggc caaaacaagg agcgggcccg agtgaggcgt aggaccctcg
     5341 cccgagccct agtcacgcaa aggagcatgc caatgcgcaa ctacctggat ggtagcaccg
     5401 gagatcgtgt acccagttcc acccccaata ccgcccccgg gacctccggg ggaccggaaa
     5461 acgccttcac cccggagtat ctggacctcc tgaatcaacg cgacgagccg ccgacggcga
     5521 gcgacgcgga tgccgccggc ccctggcatc tggagccggc gccggatggc cgctgggcgg
     5581 tgctgcgggt gggggaggcg ctcggccggg gcgatgtccc ggcgggggac ttccagatga
     5641 agcaggcggc gcagctcgcc gccgccctct ctcccgggac aggcaagagc aagcgctacc
     5701 gtcagggcaa ggaggatgac ggccggggct atcccgtcta ctacgacggc gagctggccg
     5761 gacacctccg ggtcttcgac gaggacctga tcgcgtcgat gaacgccgcc gacgccctgc
     5821 tcagcgcccc ctacgacttc gcctggctgc tcgacgccgc cggaggtctg gcgctggagc
     5881 ggacggggaa ggtgtcgctg gcccgggcga ggggggatct ttaaagaagg gggcgcttga
     5941 gctcgccacg cctgggaggg ggggcttcct ccccccctgg gcgggggcgt ggtagggagt
     6001 gggggaggac cgaggtgggg ggttgctttt ggaagggcgg gggagatccg ccacggtcga
     6061 ggacaaaaaa gctgcccccc tcctgacctc ccccactccc taccgcccac cacccagggg
     6121 gggaggcacc acctccccct gggaccagaa cctacgtctg caacaccccc gtcttgaact
     6181 ccgccaggtg cgggttcatc gccgcgcagg cgagggagcg ggcgaggatg tctcgcgtct
     6241 cggcggggtc gatgagggcg tccacccaga ggcgggcggc ggcgtagcgc gggttcgagg
     6301 cagcgtcgta gcgcgccttg atctccgaga ggcgctgctt cttctcctcc tcggagacgt
     6361 cctcgccccg gttcttgagc tggatcgaca agagcgtctt gctcgcctgg tcgcccccca
     6421 tcaccgagat cgtcgccgag ggccaggcgt agaggaaccg cgcgccgtag gccttgccgc
     6481 acatcgcgta gttgccggcg ccgaaggagt tgccgacgaa gagggtgagc ttcggcaccg
     6541 tcgagttggc gacggcgttg accatcttcg ccccgtcctt gatgatgccg ccccgctcgg
     6601 cgcggctccc caccatgaag ccggtcacgt cctggaggaa gaggaggggg atcttcttct
     6661 ggttgcacag ctcgacgaag cgcgccccct tggccgccga gtcggagtag atgacgccgc
     6721 cgatctggag ctcgcggtcc gccgggccgg tgccggtccg gcgcggcacg atggagcgct
     6781 ggttggcgac gatgcccacc gcccagccgc cgatccagcc ggtgccgcag accagcgtct
     6841 ggccatgagt ggccttgtac tcgtcgaact cggagccgtc gagcagacgc gccaggatct
     6901 cgtgcgtgtc gtagggccgc gagcggtcga tggggaccag gccgtagagg tcctcggatg
     6961 ggtagagcgg gtccgccggc gcgcggcggg cgaacggggc catctccccg gcggccatct
     7021 gcgcgaactg gctccggatc ttggccaggc acgaggcgtc gtccggatgc cggtggtcga
     7081 cgacgcccga gacgtcgcac tggacgaggg cgccgcccaa ctcctcgttg tcgatcttct
     7141 cgccgatcgc ggcctgcacc aggtgggagc cggcgaggaa gatggagccg gtcccctcga
     7201 cgatgtggga ctcgtcgctc atgatcggca ggtaggcgcc gccggccacg cacgggccca
     7261 tgatggcggc catctggaag atcccctcgg cggagaggcg ggcgttgttg tagaaggcgc
     7321 ggccgaagtg ctctttgtcg gggaagatct cgtcctgcat cggcaggaag acgcccgccg
     7381 agtccaccag gtagacgatg ggcaggcggt tctccagggc gatctcctgg gcgcgcagca
     7441 ccttcttgca ggtcaggggg aaccaggccc ccgccttcac cgtggcgtcg ttggccacca
     7501 cgacgacatc gcggccgtgg atggcgccga tgccgatgac gacgccggcc gacggggcgc
     7561 cgccgtactc cttgtagaag ccgtcggcca cccagaggcc gagctcgaga aacggcgcac
     7621 cggggtcgca gagggcggcc acccgctcgc gggcggtcat cttgccctgg gcgcgctgct
     7681 tgtcctgggc cttggcgccg ccgccgaggc ggatcctctc ctcttcctgc cgcagccgct
     7741 ccatctcccg ccgccagtga tccgcctggc gctcgaacgc ctcgccgcgg ctggtcttgc
     7801 tctccagtat cgccatcgat tcctcctcgc gggcgggatt atacgggggg gctcctgatc
     7861 ctgtaacgag tggcaggctg accacgccta ctgtcgcgca ggacccgtgt agggaggagc
     7921 ccgctcctcc cgcgccccaa actcgattcg agaggagatc ttcgatgcga ccgttcctcg
     7981 tcctcggtgt gctgctcctg tgtctgaccg tcgccgcgcc ggccctcgcc gtgactggca
     8041 ccgccgatca tgtgcccgcc gccacgctgc tggtcccgtt cttcgagacc gggatcaacg
     8101 tcaccaccca tccgcacgac acgctcatgg tggtgacgaa ctggctcttc gccgacgtca
     8161 ccttccacta ccacgtgtgg gacgtcgacg ggaacgcgac cggcctcagc ggcaacatcc
     8221 tcctggatga cctcgacacc tggagcgcgg cgatgcgcga cctcctcaac gcctcgtcgc
     8281 cggccgtccg cgcccagctc acgcggggcg ccttctaccg cggcttcgtc accatcgacg
     8341 tggtcaccgc ggcgacggcc ctgaaccccc gccaggccgg gtatccgttc ggcaccacca
     8401 acgcgctgga gggcttcacc tactacaccc ggctgtcgca gggctcggcg aacggtctgg
     8461 cgatggtgcc gctggagcat gtccccgcga cgaccgactc gttcatgagg ggcttctaca
     8521 gcgccggcga caaccgggag gagatcgact cgaccggccg cttctgtgcc aaccagctcg
     8581 ccagcaaccc gaacggcacc acgggctgca acaccgccgg ggacgacagc gacatcgacc
     8641 gcttccacct gcggatcttc cgttcggcgc cgctgaccgg caacagccgc gcggtgatct
     8701 tcacctggcg gccgggcctg gtcggcggac cgtcgatttt ctgtgacacg cagccctgcg
     8761 aatccgcgta cgtcttccgg cagtacgacg aggccggcac cgccgtgcag agctccacga
     8821 tccgtctcaa ccacgtggtg aacgtcatcg agaattcgca gctcgtgggc acgcaggcgg
     8881 ggtggctctc gatcttcgac gtgccgaaca accaggtcga tactcacgtc tacgcgttct
     8941 cgttcaactc cgccgcgccg gcggggaacc cgaacctgac ctgggacgcc atcttcgaag
     9001 gctacatcgt cccctgagag gaagccgggc cggggggagg gccttacccg tccaccccca
     9061 ccacgccgct cgacaccacc tgcagcttcc aaccctcggc ccgggtgccg tccaggtcga
     9121 cgtggccggc gaggaagaag aggcgctggg tgtccgggcg ctgcggcgtg gtgcagaagc
     9181 ggtcctggag atagaagggc gggatcgact ggtagtagag cgtcacccgc acgcccgccg
     9241 gcttccggcc gcgcagggcg ttgaggggga ccgcgtaggt cagggcgtcg ccgccccctc
     9301 ccaggccgcc cctggacttc ggctcgtacc aggggtcccg cacgtggccg ccgcggccgt
     9361 ccggcaggtc gggcagcacc ctttccacca gatcttcggc cgggagcttc gggctcccca
     9421 gcccctcccg gagcgccagc tcgagcgacg gctcccagcc gcggggcagc aggcggttgt
     9481 ccttcacctc gtcggccagg gagaggaagc tggtggtgaa ccggccgcgg gggtcgcgca
     9541 ccagctcctg atagatctgt gcctggtcct ggcgggtgat gacctcgtag tggggctgga
     9601 agtgatccct ctgctcggcg acggtcatgg gccggcagtc gctcttccag aagtgctcgc
     9661 cggggagcgg cttgcccgcc tcgtccacca gcacgccggt ggacgtgctg cggcccgaga
     9721 cccagagcgg cttgccagag ccgtccagca cctcgaaggt gaggaaggcg cgacggaatc
     9781 ccaccccgga cggcagcttg tggccggcca ggttctccac ccggacctcg gccacgaggt
     9841 cgctggcggt gcgccgcacc ccggtcaccg agaccttcgc cgtggccgtg tcggcctgct
     9901 ggatcatgga gtcgtaggtg gtctggagcg gcgccacgcc gcggcccccc agcatcgggt
     9961 cctggatgcg gatgcccagg atgtccggga actgctgggc gaagaggttg aagaagacgt
    10021 tcagccccac cagggtgtgc cggccgaacg gggagcgggg cttgaggtcg atctccgact
    10081 ccggccgccg gttctccgtc tgcggcatgt tggaagcctc ctggatggag gcgatcttga
    10141 aggacagcgg gccggtcacg ccggggtagc tcgacgccat gtggcagtcc tggcacgagc
    10201 gcggagtggc gccgtcgtcg cggaaatcgc tgaacaccca ctccggatag gtggcctgct
    10261 cgatgatgat ctccggcttg ctctcccccg gccgcaccca gggctggtcg ccgtcgaaca
    10321 ccggcagcac tacgctgtgg cagctcccgc agaccttgga cgagcggatc agcggatagg
    10381 ccatcggctt cgcccccagc gtgtgctcca tgggcacctg ctgcggccct tcgaaggggc
    10441 catggatctc ctccggcgcg ccgacccgga agtcgccggt gaaggtgtgg ccgatggggg
    10501 tcgtctggtc cacccgaatg tgatggcagg tggtgcacga gacgccgtcc cgcgccaggg
    10561 cgccgtagcg cgcctcctgc tgctgctccg gcgtcgccgc gccatagggg acggtgttca
    10621 gcgcggcgcg ggagaagagc ttccgcggca cgatgggctg cgagctctcg tccaagccca
    10681 ggaggtcggt gttgtcgcag acctcgttgg cccgcgccgg atcgcccggg aactggtcga
    10741 ggcagttctg gcgctgcccc atgacgccat ggcagtggag acagagatcc tgcaccagct
    10801 tgccgagctt cgggtggagc gtctcctcgg actcgagctg ggagaagaag atggggtcgc
    10861 gcccggcgag gcccatgggg gagccccgcc actcgccgta gggggcgagg ttgagcagct
    10921 tctggtaggc cggctggccg gcctgctgga gcgtcatgtc gaagtgcatg ccggtggagc
    10981 cggcggcgtg gcagcccagg cactggtccg aggtgaggaa gagcgcgggc ttgtcagccg
    11041 gcggggggac gctgtcgtag acctcgggcg gcatgtagag gcgggtgatc tgccggccgt
    11101 cggggggctg gatggaccgg gcgccgaagg tggcgaggaa ggcgggcgag tagctggccc
    11161 gcggctgact cacccgctgc acctcgggat tcggccaggg cacctggttc cacggcgtct
    11221 cgcccgcctc cgcgtccatg tcgcggacgg ggtcgaagcg ctcgtgggac gacagggtct
    11281 ccactggcgt cgccgctggc cggggcgccg cgccaacgat gggcggctgg tcctgggcgt
    11341 agtacttgag gaaggtgtcg gggtcgccca ggacgttgtt caggctggaa aaggtgaatt
    11401 cgtcctgcgc cgaggcgtgg cagttgacgc agtagtagcc gaagcccatc caggggtagg
    11461 ggaggttcac cggcggcggc cagtcgagct gcgacgcctt ggtcgcctgc gggtcccagg
    11521 agccccagaa ccagccgtcc cgggagcccc ggcggtcgcg caccatgagg gcgaccatgt
    11581 tcggcgggcc gtcggggatg gacgggtcct ccagccgggc ggcggtcatg ttgccgtaca
    11641 tcagcttgag gatcatggag ccgtcggcga tggggcgcac gggagcccgg tcatccagcc
    11701 gacagtccgg gcaggtcgtc cggcattgat cgacgaagcg gcgctcctcg cgggcgtcgc
    11761 gctcgcacat ccagcgatag acgtcgggcg agtagtagat ggcgaccgtg ttgtgggtgc
    11821 cccaggtggt ggggctccag gccggcgcgt ggggatcgcc cccgagggcg gcgatggtgg
    11881 ggccggtggg ccgcacctgc ttgtcgtgct tccagccgag ctcaaggtag cagcgggcgg
    11941 cgaggaaacg gccgaggagc cgctcatagg cggacttgtc ggcggggaac cggctgggca
    12001 gggggagagg ggccgagcag agcgccgccg ggtccgcgtc cgtgccgacc tccgattcag
    12061 cggccggcgc tcccacggcc atcgtcaccg ctcccgccag cagcggcacc accagcaacc
    12121 agagagcccg ccgggttccc atcgccaacc tcctcctgct cgatttttgg tggcccgcag
    12181 gatagcacgg cgcccccggc ccggtccttg cactcccagt tagccgggtg cccgcgggag
    12241 tgccgccggc acccacgtca acgacgagaa aggagatcac catggaagac gtgttcgagg
    12301 gtcgctggaa gcagctcaag ggggacgcca agcgtgcctg gggcaagctg accgacgacg
    12361 actgggaccg tgccgagggt aaccgggaca agctcgtcgg ctccctgcag gagcgttacg
    12421 ggtgggagaa ggtgcatgcc gaatccgaat tcgaccgctt cctcgagtcg atccgcacgc
    12481 cggccggcgc cggtgccggc accgaccgta ccggcgaccg ttactagata gcctggcgct
    12541 ccggcgccgt tgccgaaggg cggcccacgg gccgcccttt tgccgttcga tgtagcatct
    12601 cccgcatgaa gatccgcctg ctcgccttcg ccaccgccgc cgacgccctg gggacggacg
    12661 agaccgagat ggagctgccc gacggcagcc gggtgtccga cctgcgggcc cggctcgacc
    12721 gggagcaccc ctccctctcc cctctctggc cacgcctggc ggtggccgtc gacggccggg
    12781 tggtcccccc ggac
//
LOCUS       DASPJI010000433         9853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_660012, whole genome shotgun sequence.
ACCESSION   DASPJI010000433 DASPJI010000000
VERSION     DASPJI010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9853)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660012"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VEL74_11730"
     CDS             <1..299
                     /locus_tag="VEL74_11730"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG63243.1"
                     /translation="LLEFASDCEPQNSLYRAEMAWCRFLQSPSLPNQPVRELEETLKR
                     DPRCGLAAYYLGEVLRQSGRFAEAEEPLQRAIKLMSPDRRPIEALKALSAEKKR"
     gene            complement(296..661)
                     /locus_tag="VEL74_11735"
     CDS             complement(296..661)
                     /locus_tag="VEL74_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63244.1"
                     /translation="MAFEYVLANLLADNDQALGALFMDGTGETVDLACSEFTPYQMRV
                     VGAYLGIYLRQMEKLLASNDLGAPRLIHIEKRDIHIYAMPLPDGYFLALVQRHPALVA
                     RTRLSMEDAAEQLRREFFS"
     gene            complement(688..2460)
                     /locus_tag="VEL74_11740"
     CDS             complement(688..2460)
                     /locus_tag="VEL74_11740"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYG63245.1"
                     /translation="MKSIQRFSRLKLRHVLLIALLLSGIIPLAISSILLILQNQEVLR
                     NAEQDYLVRKAGSLSREVNSYLVNVRRQLRQIGGGVLSPPGPPEAPQTAADRLNEGWV
                     SGYLQGFLRENPDFAALGVVDPATTIGPVIAPRNISKPAVDTLKAVFGTARNGKEAVY
                     GFVKDGSGGEPLAAVAVPVAQGGSRVVVETLVKFEPLERLEQEARGGVTIFLLDGQGK
                     LLWSNGGDPVMEKSFMSLPVIRSFLRRPMAMTTEYEVDSAKGRQDVFAQVSAMEESGW
                     IMVVQKPLDETFVAVDRMIFNTVLSSLLLVALALFFAFMASRWVSLPIQRLAEKSHDI
                     AAGNFGGRVEMEGLAFELADLADDFNRMSGHVETYVQQLRQAAQANRDLFIGSLRAFV
                     AAIDAKDPYTRGHSERVAAVSRTISRYLGLPEEVQHKVWIGALLHDVGKIGVEDRILR
                     KEGVLTPEEFEQMKAHATMGADIMMPIEQLKEMIPAIRWHHEAWNGRGYPDGLKGEQI
                     PLFARIVAVADTFDAITTNRPYQQAYSLQFAVETITRLTGSRFDAKIVTAFLRAFEAG
                     EIRAGAARQSREATQVEVKAAAQR"
     gene            complement(2534..3634)
                     /gene="aroB"
                     /locus_tag="VEL74_11745"
     CDS             complement(2534..3634)
                     /gene="aroB"
                     /locus_tag="VEL74_11745"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HYG63246.1"
                     /translation="MPLHRLALRHPTGSTPVLVGEDALAAARPLLEEWLAGRTVFLIS
                     TPRVLGLHGERLAPFRQAAARWVVLEVEEGEAAKTVESAGRLWNEMLAAGGKRDSRLV
                     AFGGGSVGDLSGFVAGCFLRGIPFIQVPTTLLAQVDAAIGGKTGVDLPGGKNTVGLFH
                     HPAMVVCDTSVLSTLPREELRSGLVEVVKMAALLDPDLLDRVESGLGRLLAGDRERLG
                     PVVAAAAAAKIGVVERDPDEQGDRRLLNFGHTLGHALETACGYAGLRHGEAVGFGLLF
                     ALRLALRRGLDPAVAERLRTLLGRFGLPPLPRDLDPRELMALMARDKKAREAGLTWVL
                     PVALGEGRMVEGIGGPEVEEEIRQFLKAPLGT"
     gene            complement(3644..5428)
                     /gene="aspS"
                     /locus_tag="VEL74_11750"
     CDS             complement(3644..5428)
                     /gene="aspS"
                     /locus_tag="VEL74_11750"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008881030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HYG63247.1"
                     /translation="MKRRGAGTIRKEDIGGTVRLQAWVHRRRDLGGLLFLDLRDRSGI
                     VQVVVYPEDHPDTARALDPVRPEWVVEVEGEVVARSPETVNRDLATGEVEVVAHRAAV
                     LSRSEPPPFPLDSKAEVSEDLRLRYRYLDLRRPELQRNLLLRDRVTLEVRNYFHEHGF
                     VDVETPILTRSTPEGARDYLVPSRVHRGSFYALPQSPQLFKQLLMVGGLERYVQIARC
                     FRDEDLRADRQPEFTQVDVEMSFPTEEDVIELIEGLFTRIFPLGGIEPPVPFPRMTYA
                     EAIARYGSDRPDLRFGLEIADLTARLGESGFRGFKETVEKGGVIRGFTVPGASEASRK
                     DVDGWAEIARRSGAAGVLTLRRKGGETVFQVKNALTPEELEGAAQALGLEEGGLALIV
                     AAPAAVAAPALGTLRLELARHYKLIPPGSHAFLWVTEFPLVEWNEEDGRWFSTHHPFT
                     SPDPRDMEILESDPGAVRSRAYDVVLNGIELGGGSIRIHDQAVQSRVFQVLGIDPEEA
                     KARFGFFLDALRYGAPPHGGIALGLDRMVMLLSGGSSIREVMAFPKTASASDLMTDAP
                     SEVDERQLRELGIALRGAERRGEDREEG"
     gene            complement(5467..5997)
                     /locus_tag="VEL74_11755"
     CDS             complement(5467..5997)
                     /locus_tag="VEL74_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HYG63248.1"
                     /translation="MPIHPYQGRLPKLGSRVFLAPGAHVVGDVELGDDVSFWFHTVAR
                     GDVNYIRVGAGTNIQDGSVLHVNHGTHPLVIGERVVVGHQAVVHGCTIEDGALIGIGA
                     QVLDGAVVERGAQVGAGAVVPPRMRVPAGHLVLGVPAKVARPLTDEEIRRIGENADRY
                     IALKDEYLTVFGTGQG"
     gene            6154..6873
                     /locus_tag="VEL74_11760"
     CDS             6154..6873
                     /locus_tag="VEL74_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYG63249.1"
                     /translation="MLRPGVPSPSLTIDVVIPALNEEASLPLVLADLPRSMVRRVVVG
                     DNGSVDGTARVAREGGAEVVEAPRRGYGSACLAALDHLRRIGPPDVVVFVDADYSDHP
                     EELPHLVAPILSGEADVVIGSRVLGEREPGALLPQARAGNLVACLLIRILYGHRYTDL
                     GPFRAARWDALERVGMSDPNFGWTAELQVKALRHGLRVTEVPVSYRRRVGVSKITGTV
                     TGTLRAGYKILWTVLRYSRGR"
     gene            6879..7301
                     /locus_tag="VEL74_11765"
     CDS             6879..7301
                     /locus_tag="VEL74_11765"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012959243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HYG63250.1"
                     /translation="MSEATRHVTDVEVRYAETDQMGVVHHANYIVWFELARTGLCALS
                     GHHYADIEKMGYLLMVTGVEVRYRRPARYGDVVQVVCWNERLGSRGLRFAYEVRKGGE
                     VLVTGATDHVWVESATGRPCRAPEVVQERFRELAGMTS"
     gene            7480..8130
                     /locus_tag="VEL74_11770"
     CDS             7480..8130
                     /locus_tag="VEL74_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYG63251.1"
                     /translation="MIRVVVADDQAIVREGVAALLRLQADMDVVGEAGSVGETIDLTL
                     RHRPDVVVIDISMPVGGGLRAVTQLREAGVQSRFLTLTMHQEPAIVRSAITAGVTGYV
                     LKSDGITELGAAIRAVHQGRSYINVPLAPGGLESMLGSSARPVNGRGAALSRREEEVL
                     KLLAAGHTYRAIAASLHVSEKSVETYRARLWEKLHLQSRVDLIRYAMLHGLLDEAS"
     gene            complement(8137..>9853)
                     /locus_tag="VEL74_11775"
     CDS             complement(8137..>9853)
                     /locus_tag="VEL74_11775"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HYG63252.1"
                     /translation="LEADQHRDRLLAGLRAAEGNPTLQRDSVDELRQRFASYYTMARG
                     TSARMIRGDAGLDMVASLREMTNRYNELQRGIAGLTESSRLGMRAAFGEEQSNQRFAV
                     WAIILVTLVCLLTLAGISLYTARSLVLPVREAVGVARRLAEGDFTADIAPRSQDEIGE
                     LTSAMQRVVTYLREMALVADGIAGGELRRSVTPRSEADLFGNALQRMTLRLRQVIGDV
                     KASAGQLATTADELSASALQIKHGAEVQSTSTEETSATMVEMATQLDSVNRSTQALAA
                     NAVATSESILLMNGAIEEVAEGSERLLGDVSQTSAVIEQMAATTRAVAGRVAVMEEVS
                     QETSQAVTAGGERLVSIVQSIGSSAKDIGKIVRTIEEFADQTNLLALNAAIEAARAGE
                     AGRGFAVVAEEVKRLAERSMQSTREITTFVDTAQRDTQEAVAVSRTIVQQIVAAVGRT
                     TELVRDVHTATRDQSEGAAQILATATTMHEVTRNLAETAREQAEEARKITGSVEAMNQ
                     MTHQVAAATEEQMRGGDQVVKAMEQIAQVAQHYLGATEQMSTAARDLAVEAERLKDMA
                     AVFQA"
BASE COUNT         1469 a         3496 c         3391 g         1497 t
ORIGIN      
        1 aactcctgga gttcgcctcg gactgcgagc cgcagaacag cctctaccgg gcggagatgg
       61 cctggtgccg cttcctccag tcgccctccc tccccaacca gccggtccgg gagctggagg
      121 agaccctcaa gcgcgacccg cgttgcggcc tggccgccta ctacctgggc gaggtgctgc
      181 ggcagagcgg ccgcttcgcc gaggccgagg agcccctgca gcgcgccatc aagctcatga
      241 gccccgaccg gcggccgatc gaggcgttga aggctctgtc ggccgagaaa aaacgttagg
      301 agaagaactc gcggcggagc tgctccgcgg cgtcttccat gctgaggcgg gtgcgggcga
      361 ccagggcggg gtggcgctgc accagggcca ggaagtagcc gtcgggcagt ggcatggcgt
      421 agatgtggat gtcgcgcttc tcgatgtgga tcaggcgcgg cgctcccagg tcgttgctcg
      481 cgaggagctt ctccatctgc cgcaggtaga tgccgagata ggcccccacc acccgcatct
      541 gatagggggt gaactcggag caggcgaggt cgacggtctc tccggtgccg tccatgaaca
      601 gggcgcccaa ggcctgatcg ttgtcggcga ggaggttggc gaggacgtac tcgaaagcca
      661 ttcagatgcc cggcccgctt gttctcatca acgctgtgcc gccgccttca cctcgacctg
      721 cgtcgcctcc cgcgactggc gcgcggcgcc ggcgcggatc tccccggcct cgaaggcgcg
      781 caggaaggcg gtcacgatct tggcgtcgaa gcggctcccg gtgaggcggg tgatggtctc
      841 gacggcgaac tgcaggctgt aggcctgctg gtagggacgg ttggtggtga tggcgtcgaa
      901 cgtgtcggcc accgccacga tgcgggcgaa gagggggatc tgctcgccct tcaagccgtc
      961 cggatagccc cggccgttcc aggcctcgtg atgccagcgg atggccggga tcatctcctt
     1021 gagctgctcg atcggcatca tgatgtcggc gcccatggtg gcgtgcgcct tcatctgctc
     1081 gaactcctcg ggggtgagga ccccctcctt gcgcaggatg cggtcctcga cgccgatctt
     1141 gcccacgtcg tggagcagcg cgccgatcca caccttgtgc tggacctcct cgggcaggcc
     1201 gagatagcgg gagatggtgc ggctcacggc cgccacccgc tcggagtggc cgcgggtgta
     1261 agggtcctta gcgtcgatcg ccgcgacgaa ggcgcgcaac gacccgatga acaggtcgcg
     1321 gttcgcctgg gccgcctgac ggagctgctg cacgtacgtc tccacgtgac cgctcatgcg
     1381 gttgaaatca tcggcgaggt cggccagctc gaaggcgagc ccctccatct ccacccggcc
     1441 gccgaagttg ccggctgcga tgtcgtggct cttctcggcc aggcgctgga tcggcaggct
     1501 cacccaccgc gacgccatga aggcgaagaa gagcgccagc gcgaccagaa gcagggatga
     1561 gagcacggtg ttgaagatca tccggtcgac ggcgacgaag gtctcgtcca gcggcttctg
     1621 caccaccatg atccagccgc tctcttccat cgcgctgacc tgggcgaaga cgtcctggcg
     1681 ccccttggcg gagtcgacct cgtactccgt cgtcatcgcc atgggacggc gcaggaacga
     1741 ccggatcacc ggcaggctca tgaacgactt ctccatcacc gggtcgccgc cgttcgacca
     1801 caggagcttg ccctggccgt ccagcaggaa gatggtgacg ccgccccgcg cctcctgctc
     1861 gagcctctcc aggggctcga acttcaccag cgtctcgacc accaccctgg agccgccctg
     1921 cgccaccggc accgcgacgg ccgccaacgg ctcaccgccg gagccgtcct tgacgaagcc
     1981 gtagaccgcc tccttgccgt tccgggccgt gccgaacacc gccttgaggg tgtcgaccgc
     2041 gggcttgctg atgttgcgcg gggcgatgac agggccgatg gtggtcgcgg gatccaccac
     2101 tcccagcgcc gcgaagtccg ggttctcccg gaggaagccc tgcagatagc cgctgaccca
     2161 cccctcgttc aggcggtcgg ccgccgtctg cggcgcctcg ggcgggcccg gcggcgacag
     2221 cacgccgccg ccgatctgcc ggagctgccg gcggacgttg accaggtagg agttgacctc
     2281 ccgcgacagc gagccggcct tgcggaccag gtagtcctgc tcggcgttgc gcagcacctc
     2341 ctggttctgg aggatcagca ggatgctgct gatggcaagc gggatgatgc ccgacagcag
     2401 cagtgcgatc aggaggacgt gccgaagctt caatcggctg aatcgctgga tgctcttcat
     2461 gcggggccgg ctgcttgagc gctcgatcct cgaaggttac tcacagattt ctatgagtat
     2521 accgcattcg gggtcacgtt ccgagcgggg ctttcagaaa ctgccggatc tcctcctcca
     2581 cctccggccc gccgatcccc tcgaccatcc tgccctcccc cagcgctacg gggagcaccc
     2641 aggtcaggcc cgcctcgcgc gccttcttgt cccgcgccat cagcgccatc agctcgcgcg
     2701 gatcgagatc gcgcggcagc ggcggcaggc cgaagcgccc gaggagggtg cggagccgct
     2761 cggccacggc gggatcgagg ccgcgccgca gggccagccg cagggcgaag aggaggccga
     2821 agcccaccgc ctctccgtgg cgcagcccgg cgtagccgca ggccgtctcc agggcgtgcc
     2881 ccagggtgtg gccgaagttg aggaggcgcc ggtccccctg ctcgtcgggg tcccgctcga
     2941 cgacgccgat cttggccgcc gcggcggcgg ccaccaccgg acccagccgc tcccggtcgc
     3001 cggcgagcag ccggcccagg ccggactcca cccggtcgag gaggtcggga tcgaggagcg
     3061 ccgccatctt gaccacctcc accagcccgg agcgcagctc ctcccgggga agggtcgaca
     3121 ggacggaggt gtcgcagacc accatcgccg ggtggtggaa cagcccgacg gtgttcttgc
     3181 cgcccggcag gtcgacgccg gtcttgccgc cgatggcggc gtccacctgg gcgagaaggg
     3241 tggtgggcac ctggatgaag gggataccgc gcaggaagca gccggcgacg aagccggaga
     3301 ggtcgccgac gctgccgccg ccgaaggcga cgagccggct gtcgcgcttg ccgccggcgg
     3361 cgagcatctc gttccacagc cggccggccg actccacggt cttggccgcc tccccctcct
     3421 ccacctccag caccacccag cgggcggcgg cctgccggaa gggcgcgagc cgctcaccat
     3481 gcagaccgag cacccgcggc gtggagatca ggaagacggt acggccggcg agccactcct
     3541 cgagaagagg acgcgccgcg gcgagggcgt cctcccccac caggacgggg gtggagccgg
     3601 tggggtggcg gagggcgagc cggtggagcg gcaaggcctt gcctcagccc tcctcgcgat
     3661 cctcgccgcg ccgctccgca ccccgcaacg cgatccccag ctcccggagc tgccgctcgt
     3721 ccacctcgga cggcgcgtcg gtcatcaggt cgctcgcgga ggcggtcttg gggaaggcca
     3781 tgacctcacg gatggagctg ccgccggaga ggagcatcac catacggtcc aggcccaggg
     3841 cgatgccgcc gtgcggcggc gcgccgtagc gcagggcgtc caggaagaag ccgaagcggg
     3901 ccttcgcctc ctccggatcg atgcccagca cctggaagac ccggctctgc accgcctggt
     3961 cgtggatacg gatggagccg ccgcccagct cgatgccgtt cagcaccacg tcgtaggcgc
     4021 gggagcggac cgcgccggga tcgctctcca ggatctccat atcccgcggg tccgggctgg
     4081 tgaacgggtg atgcgtggag aaccagcgcc cgtcctcctc gttccactcc accaggggga
     4141 actcggtgac ccagaggaag gcgtggctcc ccggggggat cagcttgtag tggcgcgcca
     4201 gctccagacg cagggtgccg agggccggcg ccgccaccgc ggccggcgcg gcgacgatca
     4261 gggccagccc cccctcttcg aggccgagcg cctgggcggc cccttcgagc tcctccgggg
     4321 tcagggcgtt cttgacctgg aagacggtct cccccccctt gcggcgcagg gtgagcacgc
     4381 cggccgcgcc gctgcggcgg gcgatctcgg cccagccatc gacgtccttg cggctcgcct
     4441 cggaagctcc cggcacggtg aagccgcgga tgacgccccc cttctccacc gtctccttga
     4501 agccgcggaa gccgctctcc cccagccgcg ccgtgaggtc ggcgatctcc aggccgaagc
     4561 gcaggtccgg ccggtcgctg ccgtagcggg cgatggcttc ggcataggtc atccggggga
     4621 acggcaccgg cggctcgatg ccgccgaggg ggaagatgcg ggtgaacagc ccctcgatca
     4681 gctcgatgac gtcctcctcc gtcgggaagg acatctccac gtccacctgg gtgaactcgg
     4741 gctgccggtc ggcccgcagg tcctcgtcgc ggaagcagcg ggcaatctgg acgtagcgtt
     4801 cgaggccgcc gaccatcagg agctgcttga agagctgcgg cgactgcggc agcgcgtaga
     4861 agctgccgcg gtggacccgc gacggcacca ggtagtcgcg cgccccctcg ggcgtcgagc
     4921 gggtgaggat cggcgtctcc acgtcgacga agccgtgctc gtggaagtag ttgcggacct
     4981 cgagcgtcac ccggtcgcgc agcagcaggt tgcgctgcag ctcggggcgg cgcaggtcga
     5041 ggtagcggta gcggagacgc aggtcctcgg agacctcggc cttgctgtcg agcgggaacg
     5101 gcggcggctc ggagcgggag aggacggcgg cgcggtgggc caccacctcg acctcgcccg
     5161 tggccaggtc gcggttgacc gtctccgggg agcgggccac cacctcccct tccacctcga
     5221 ccacccactc ggggcgcacc gggtccaggg cccgggccgt gtccggatgg tcctcgggat
     5281 agaccacgac ctggacgatg ccgctccggt cccggaggtc gagaaagagg aggccgccca
     5341 agtcgcggcg gcgatgcacc caagcctgga gcctgaccgt gccgccgatg tcctctttcc
     5401 ggatagtgcc cgcgcctcgc cgcttcatac cgtttccttt cctttcgaca atgccactca
     5461 cctcgcctat ccctgtccgg tcccgaagac cgtgaggtac tcgtccttca aggcgatata
     5521 gcggtcggcg ttctcgccga tccggcggat ctcctcgtcg gtcagcgggc gcgcgacctt
     5581 ggcggggacg cccagcacca gatgccccgc cggcacccgc atgcgggggg gcaccacggc
     5641 tccggcccct acctgggcgc cgcgctcgac gaccgcgccg tccagcacct gggcgccgat
     5701 gccgatcagc gcgccgtcct cgatggtgca gccgtggacc accgcctggt gccccaccac
     5761 cacccgctcg ccgatgacca gcggatgggt gccgtggttg acgtggagca ccgagccgtc
     5821 ctggatgttg gtgccggcgc cgacccggat gtagttgacg tcgccgcgtg ccaccgtgtg
     5881 aaaccagaac gacacgtcgt cgcccagctc cacgtcgccg accacatggg cgccgggcgc
     5941 caggaagaca cggctcccca gcttgggaag acggccctga taggggtgga tcggcatacg
     6001 cacctcgtac gggagatctc tatctatata gatggagccc cggcccggag gccggcaggg
     6061 gggagagcat ggcacaaagg gcagggggct tccaagcgag gagaaggacc cgggaccgct
     6121 ccgccagcct ccccttcggc ataaactccg ccggtgctcc ggcccggcgt cccctccccc
     6181 tccctgacca tcgacgtggt catcccggcc ctcaacgagg aggcgtcgct ccccctcgtc
     6241 ctggccgacc tgccgcgttc catggtccgc cgggtggtgg tgggcgacaa cggctcggtg
     6301 gacggcacgg cgcgggtggc gcgggagggc ggggccgagg tggtggaggc gccgcggcgg
     6361 gggtatggca gcgcctgcct cgccgccctc gatcacctgc ggcgcatcgg ccccccggac
     6421 gtggtggtct tcgtcgacgc cgactactcg gaccaccccg aagagctgcc gcacctggtg
     6481 gcgccgatcc tctccggcga ggccgacgtg gtcatcggct cgagggtgct gggcgagcgc
     6541 gagccggggg ccctcctccc ccaggcgcgg gccggcaacc tggtggcctg cctgctgatc
     6601 cggatcctct acgggcaccg ctacaccgac ctgggcccgt tccgtgccgc ccgctgggac
     6661 gccctggagc gcgtcggcat gtccgacccg aacttcggct ggacggccga gctccaggtc
     6721 aaggccctgc ggcatgggct acgggtgacg gaggtccccg tctcctaccg ccgccgcgtg
     6781 ggcgtctcga aaatcaccgg caccgtcacc ggcaccctcc gggccggcta caagatcctc
     6841 tggacagtgc tccgttacag ccgagggaga tgagagagat gtccgaagcg acccgccacg
     6901 tgaccgacgt cgaggtgcgc tacgccgaga ccgaccagat gggcgtcgtc caccacgcca
     6961 actacatcgt ctggttcgag ttggcgcgca ccggcctgtg cgccctgtcg ggccatcact
     7021 acgccgacat cgagaagatg ggctatctcc tgatggtgac cggcgtcgag gtgcgctacc
     7081 ggcgccccgc ccgctacggc gacgtggtcc aggtggtctg ctggaacgag cgcctgggca
     7141 gccgcggcct ccgcttcgcc tacgaggtcc gcaagggggg cgaggtcctg gtcaccggcg
     7201 ccaccgatca cgtctgggtc gaatccgcca ccggccgccc ctgccgcgcc cccgaggtcg
     7261 tccaggagag attccgtgag ctggccggaa tgacgtctta gcgctcccct ggagttgcca
     7321 tttcgccgaa atactgaatc ccgcctccta cttgcaggcc ccgccggaaa gccctatgat
     7381 gctgtaacaa agctcgtaac acttggtttt gtctctccga gactggagaa ggctcctctc
     7441 ggtctggaga cttgtttttt ctaccgatgg agtaaaaaca tgatccgggt cgtagtggcc
     7501 gatgatcaag cgatcgtccg ggagggggtc gctgctctgc tccggctaca ggccgacatg
     7561 gacgtggtcg gggaggcggg cagtgtcggc gaaaccatcg acttgacgct acggcaccgt
     7621 cccgatgtcg tggtgatcga catctcgatg ccggtcggcg gcggcctgcg ggccgtcacg
     7681 cagctccgcg aagccggagt gcagagccgg ttcctgacgc tcacgatgca tcaggagccg
     7741 gccatcgtcc gttcggcgat caccgccggc gtcacgggat acgtcctcaa gagcgacggc
     7801 atcaccgagc tcggcgccgc catccgtgcc gtccaccaag ggcgctcgta catcaacgtc
     7861 cctctggcgc ccggcggcct cgagtccatg ctgggcagct cggcacgccc ggtcaacggc
     7921 cggggcgccg ccctgagccg gcgcgaggag gaggtgctga agctgctggc cgccggccac
     7981 acctaccgcg ccatcgccgc gtcgctccac gtcagcgaga agtcagtgga gacctaccgc
     8041 gcacgcctct gggagaagct ccacctccag agccgggtcg acctgatccg ctacgccatg
     8101 ctccatgggc tgctcgacga ggcgtcctga tccgcctcag gcctgaaaga cggccgccat
     8161 gtccttgagc ctctcggcct cgaccgccaa gtcgcgcgcc gcggtggaca tctgctcggt
     8221 cgcgcccaga tagtgctggg ccacctgggc gatctgctcc atcgccttga ccacctgatc
     8281 gccgccgcgc atctgctcct cggtggcggc ggccacctga tgcgtcatct ggttcatcgc
     8341 ctcgaccgag ccggtgatct tgcgggcctc ctcggcctgc tcccgcgccg tctccgccag
     8401 attccgggtc acctcgtgca tggtggtggc ggtcgccagg atctgcgccg ccccctccga
     8461 ctgatcacgg gtggcagtgt ggacgtcgcg gacgagctcg gtggtccggc ccaccgccgc
     8521 cacgatctgc tggacgatgg tccgggagac agccaccgcc tcctgtgtat cgcgctgggc
     8581 ggtgtccacg aaggtggtga tctcacgggt cgactgcatg gagcgctcgg cgagccgctt
     8641 gacctcctcg gcgacgacgg cgaagccgcg gccggcctcg cccgcgcggg cggcctcgat
     8701 ggcggcgttg agcgccagga gattggtctg atcggcgaac tcctcaatgg tgcggacgat
     8761 cttgccgatg tccttggccg acgagccgat ggactggacg atggacacca gcctctcgcc
     8821 gccggcggtg accgcctgtg acgtctcctg cgaaacctcc tccatcaccg ccactcgtcc
     8881 ggccaccgcc cgcgtcgtcg ccgccatctg ctcgatgacc gcggacgtct ggctgacgtc
     8941 cccgagcagg cgctccgagc cctccgccac ctcctcgatg gcgccgttca tcaacaggat
     9001 cgattccgag gtggcgaccg cgttggccgc cagcgcctgg gtcgaacggt tgacgctgtc
     9061 gagctgcgtc gccatctcca ccatggtggc cgaggtctcc tcggtggagg tcgactggac
     9121 ctcggcgcca tgcttgatct gcagcgccga ggccgacagc tcgtcggcgg tggtcgcgag
     9181 ctgccccgcc gaagccttga cgtctccgat cacctggcgc agccgcaggg tcatgcgttg
     9241 cagcgcgttg ccgaagaggt cggcttcgga gcggggcgtg acgctgcgcc gcagctcgcc
     9301 gccggcgatg ccgtcggcga ccagagccat ctcccgcaaa taggtgacga cgcgttgcat
     9361 cgccgaggtg agctcgccga tctcgtcctg cgagcgcggt gcgatgtcgg cggtgaagtc
     9421 gccctccgcg agacgccggg cgacgcccac cgcctcgcgc accggcagca cgagggagcg
     9481 cgcggtataa agggagatgc cggcgagggt caggagacag acgagggtga cgaggatgat
     9541 cgcccacacc gcgaagcgct gattcgactg ctcttcgccg aaggcggccc gcatccccag
     9601 gcggctgctc tcggtcaagc cggcgatccc ccgctggagc tcgttgtagc ggttcgtcat
     9661 ctcgcgcagg gaggcgacca tgtcgagccc cgcgtcgcca cggatcatac gggcggaggt
     9721 gccgcgcgcc atggtgtagt agctcgcgaa ccgctgccgc agttcatcca ccgagtcccg
     9781 ctgcaaggtc gggttcccct cggccgcccg caggccggcc agcaaccggt cccggtgctg
     9841 atcggcctcc agg
//
LOCUS       DASPJI010000434         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_372084, whole genome shotgun sequence.
ACCESSION   DASPJI010000434 DASPJI010000000
VERSION     DASPJI010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372084"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1556
                     /locus_tag="VEL74_11780"
     CDS             216..1556
                     /locus_tag="VEL74_11780"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HYG63253.1"
                     /translation="MFWQYLWFGIMVLLVFGGPTFALGHGRKRLKKWRQAAESSRLTV
                     VKDSNLPLVRHLTARSGPVEMRLSEQKDDVRVVASIMEGPPDLFEVTIRREVLPLPGR
                     REIKTGDEKFDDAFVIKGPQPFVRALLDASTRRMLMEAKIEGWVELVDGELRLDFREA
                     KLAHVLGLFRDLGSRFADQQELPRRLLENVRHDPEAGVRLQNLLVLIREYPGDPSTVR
                     ALHVGTKDSNPEVRLRAAQALGAEGRDVLQELAKGLKEDAVSAQAVTALGLALPLERL
                     QSILGGARQKRMTETARACLEALAILGGAAVGTLVKVLDDDSSELAATAARGLGKIGD
                     PAAEPPLLAALERDNPDLQEAAAEALGRVGSAAAVLPLQEAADRSSALHDAALQAIAG
                     IQERLQGASPGQLSLAATDAGQLSLADDAGQLSLAPHPTEQLPLPTPPPPQRTR"
BASE COUNT          349 a          777 c          797 g          360 t
ORIGIN      
        1 gccgaatcgg ggacctgtcc cacggactgc cagaactccg acctctgccc cccgcagacc
       61 aatggctgcc cccctggcgg aaccggctgt acctgtacgt gcccgccgac ctccttcatc
      121 gaggggagct gcagctgctg atcggctgcc tctctgtcat cgctgccgga ttgaggccgc
      181 tggcgggcgc ctgcgccgcc ggataaactg cggggatgtt ctggcagtat ttgtggttcg
      241 ggatcatggt gctgctggtc ttcggcggcc cgacgttcgc cttggggcat ggccgcaagc
      301 gcctgaaaaa atggcggcag gccgcggaga gcagccgcct gaccgtggtg aaggattcca
      361 acctgccgct ggtccgtcac ctcacggctc gctcgggacc ggtggagatg cggctctccg
      421 aacagaaaga cgacgtccgc gtggtggcgt cgattatgga ggggccgcca gatctttttg
      481 aggtgacgat ccgccgcgag gtcctcccgc tgcccggcag gcgcgagatc aagaccggcg
      541 acgagaagtt cgacgacgct ttcgtgatca aggggccgca gccgttcgtc cgcgcgctgc
      601 tcgacgcgtc gacgcgccgc atgctgatgg aggcgaagat cgagggctgg gtcgagctgg
      661 tggacgggga gctgcggctc gattttcggg aggccaagct cgcccatgtc ctgggcctct
      721 tccgggacct cggcagccgc ttcgcggacc agcaggagct gccgcggcgc ctgctggaga
      781 acgtccgcca cgaccccgag gccggcgtgc ggctccagaa cctgctcgtg ctgatccgtg
      841 agtaccccgg ggatccgagc accgtccggg cgctccacgt cggcacgaag gattccaacc
      901 cggaggtccg gctgcgggcg gctcaggcgc tcggagccga aggacgcgac gtcctccagg
      961 aactggccaa ggggctgaag gaggatgcgg tgagcgccca ggcggtgacg gccctcgggc
     1021 tggcgctgcc cctggagcgc ctccagtcga tcctcggcgg cgcgcggcaa aagcgcatga
     1081 ccgagaccgc ccgcgcctgc ctggaagcgc tcgcgatcct cggcggcgcg gctgtcggga
     1141 cgctggtgaa ggtgctcgac gacgacagct ccgagctcgc cgcgaccgcg gcccgtgggc
     1201 tcggcaagat cggcgaccct gccgccgagc cgccgctcct cgcggctctg gagcgcgaca
     1261 acccggacct gcaggaagcc gcggccgagg ccctgggccg cgtcggctcg gccgccgccg
     1321 tgctgccgct ccaggaagcg gccgaccgtt ccagcgcact ccacgatgcc gcgctccaag
     1381 ccatcgccgg gatccaggag cggctccagg gcgcctcgcc cggccaactg tcgctggcgg
     1441 cgaccgacgc cggtcaactc tccctggccg acgacgccgg ccagctctcg ctcgcccccc
     1501 atccaaccga acagctcccg ctcccgactc cgccaccacc gcagagaacc cgctgaccca
     1561 ctacctcaca gcccgagggg gcggtggtgc ctccccccct gggtggtggg cggtagggag
     1621 cgggggaggt gaaggagggg gggttttctt tttctctcgc cgtcttgact ccgcggcggc
     1681 cgtccccatc gaaatgacga ccatcctcga gcgaatgacc gctatacccc gggtaggagc
     1741 gccgtccccg gagggaggac ggccgtacct cgggtacggc ggccatacct ggagggatgg
     1801 cggtcgtgcc cacgggaagc atgccgtccc caccggtagg acctctttct cgcgggtacg
     1861 gcgaccatcc ctggagggat gacggccgtc ccgggttggg caatcgtcga gcggcccatt
     1921 ccaccggggt tttgggaggc ggagacgttc ctccgcctcc cacgacagag gcgccgcgct
     1981 tcaggggtgc ggccttggac ccggcggcac ccctgtgcga ttcagttgtc aaagaactcg
     2041 gcagcggtct cccggcccgg aggcaaggga agtcgctgag cgagcagaat tgtaggcgaa
     2101 tcgagactgt ttggctagat cgccttgaca tttcaggctg tcatgatgac aatagaggcg
     2161 acggaggcga attgacggcc tccgtcaggc ggttctttga caattgatgg accaagtctc
     2221 gggcacgacg agggcggcgc cgtgcccggg gcctgggttg tcttactctt ctcctatcgg
     2281 ctg
//
LOCUS       DASPJI010000435         4501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_595991, whole genome shotgun sequence.
ACCESSION   DASPJI010000435 DASPJI010000000
VERSION     DASPJI010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595991"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..1450)
                     /locus_tag="VEL74_11785"
     CDS             complement(203..1450)
                     /locus_tag="VEL74_11785"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HYG63254.1"
                     /translation="MKTRLAASALLALAVLCGAAPAAAQDKTLRLAVGDPARKGREAP
                     VVLDAVTDTRTGDLLTPAQMAARLAGARLVLVGESHTDVDFHRAQLRVIQELRKAGRQ
                     VLIGLEMYPYPEQPFLDQWTRGLLTEQGFVELSRWYGNWGYHWDYYRDIFLYARDHGL
                     PMFAVNAPRAVVTAVRQKGFDSLTPEEAAHVPRRVDTASDEHRALFRAYFGDDDPLHG
                     GMSPEQLDGMFAAQATWDAAMAHNALKALGRHGGPNAVMVVLAGTGHVAYGLGIQRQA
                     LVQDPALQGKVATVVPVPVRDGDGHAVAAVQASYADFVWGLPPQAAPLYPSLGLSMAS
                     GEGEAGRKVIFVAEGSAAARAGFQVGDLLLAMDGAPLTDKESFHRALAAKRWGDAASF
                     SVRRGDAALDLQAVFRRQATLSE"
     gene            complement(1463..2521)
                     /locus_tag="VEL74_11790"
     CDS             complement(1463..2521)
                     /locus_tag="VEL74_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HYG63255.1"
                     /translation="MGILSLPIEEILASGIAPPSGRLIGRSRQGRDIVGHVLGTGGPH
                     VSLIGGCHSDEPVGPEMLRRLAGFLAALPADHPLLISFTWYVVPHVNPDGEAVNSAWS
                     GHPLPAVDSRGAPDRVYDLGLYLRHVMREPPGDDMEFGFPRDEEDAGARPENRAVAAF
                     LSEGAPFALHGSFHGMAFATGPWFLIERAWTDRTAGLRESLRRRVRDLGYRVFDVDRG
                     GEKGFSRIDEGFTTRPDSVAMRAYFLEKGDPAMAERFRPSSMEYVRSLGGDPFTLVSE
                     MPLFLRPLEEGESGRPDDPRFRAWLERMATLRDAEEVRAEAERQGVRGMPLRDQMRLQ
                     LAFLEEALRAVASAAASR"
     gene            complement(2528..2701)
                     /locus_tag="VEL74_11795"
     CDS             complement(2528..2701)
                     /locus_tag="VEL74_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63256.1"
                     /translation="MKKKSVKKLHLNMETLNRMNLEAVTGGATFLPCQERTRDYTNCP
                     MCGQTNYISCLNC"
     gene            2917..3624
                     /locus_tag="VEL74_11800"
     CDS             2917..3624
                     /locus_tag="VEL74_11800"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HYG63257.1"
                     /translation="MQISAFTDRHTPEELLGPLNANELRHAPERLFLAGDTTLLWSAP
                     QVAVIGSRNPSDLGRWHAARLARFLTDEGITIVGGLSTGIETAAHETALQGLGRTIAV
                     LAAPLGVPPSPRNAELHRQILERHLVVSQFPAGHPVTREDLRRRNCTMALLSDALVLV
                     EAADGSASLEVAWEMLRLRRPVFIPRPVADAGSLEWPEEMLAQGASVLDRPAALLAAA
                     VRGSARRLSATTLRLAS"
     gene            3726..4304
                     /locus_tag="VEL74_11805"
     CDS             3726..4304
                     /locus_tag="VEL74_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015213196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HYG63258.1"
                     /translation="MAVSVRKRLLTIDEYHQMVESGILAEGERVELIYGEILEMNPIG
                     HRHATCLRRLIRLFGPGVVSDSVAPDVLLDVQNPLRLPNEQSEPQPDLMLLRGRDDCY
                     ASKPPAAEDVLLLVEIADHSLAYDRDVKLPLYAQAGIPEVWLVDLTSDTIRVHRRPSP
                     EGYLEARQAGRGEAIAPQALPRLLVRVELVLG"
     gene            complement(4313..>4501)
                     /locus_tag="VEL74_11810"
     CDS             complement(4313..>4501)
                     /locus_tag="VEL74_11810"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009146507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HYG63259.1"
                     /translation="PFEEMTAFVIACGGDVDTIGAMAGALWGAANGSAGLPPVKVEKR
                     SYLEDVADRLFRRFVGGG"
BASE COUNT          668 a         1616 c         1563 g          654 t
ORIGIN      
        1 agaacagacc tgcacacacg cgccatagac tcctcccgga taagaggcgc tgaacggaaa
       61 cgacagcggt ttgccctagg tgtacgagcg ggggaaaacg tttcacccgc ccgtccgggc
      121 caacccgatg ttcgctaggt acggcgctga agtgcgcgag agggggtcaa gcgggacaag
      181 aacagaaaaa atagctgaat cgctattcag atagcgtcgc ctgcctccgg aacacggcct
      241 ggaggtccag cgccgcatcg ccccgccgga cggagaagct ggcggcgtcc ccccagcgct
      301 tggcggccag ggcgcggtgg aaggactcct tatccgtgag aggcgcgccg tccatggcga
      361 ggaggaggtc ccccacctgg aagccggcgc gggccgcggc cgagccctcg gcgacgaaga
      421 tcaccttgcg gcccgcctcc ccctcgcccg aagccatgga gaggccgagg ctcgggtaga
      481 ggggagcggc ctgcggcggc agcccccaga cgaagtcggc gtaggaggcc tggacggcgg
      541 caaccgcgtg gccgtcgccg tcgcgcaccg gcaccgggac gacggtggcg accttcccct
      601 gcagtgccgg gtcctggacc agcgcctggc gctggatgcc caggccgtag gccacgtgtc
      661 cggtgccggc gaggacgacc atgaccgcgt tcggcccgcc gtgccgcccg agggccttaa
      721 gggcgttgtg ggccatggcg gcgtcccagg tggcctgggc ggcgaacatc ccgtcgagct
      781 gctccgggct catgccgccg tggagcgggt cgtcgtcccc gaagtaggcg cggaagaggg
      841 cgcggtgctc gtcgctcgcc gtgtccaccc gccgcgggac gtgcgcggcc tcctcggggg
      901 tcaggctgtc gaaccccttc tgccgcactg cggtgaccac ggcgcggggg gcgttgacgg
      961 cgaacatcgg caggccgtgg tcgcgggcat agaggaagat gtcgcggtag tagtcccagt
     1021 ggtagcccca gttgccgtac cagcgggaga gctcgacgaa cccctgctcg gtgaggaggc
     1081 cgcgggtcca ctggtcgagg aacggctgct cggggtaggg gtacatctcg aggccgatga
     1141 gcacctgccg tcccgccttg cggagctcct ggatcacccg cagctgcgcc cggtggaaat
     1201 cgacgtcggt atggctctcc cccaccagga cgagccgggc gccggccagc cgcgccgcca
     1261 tctgcgccgg ggtgaggagg tcgcccgtcc gggtgtcggt gaccgcgtcg agcaccaccg
     1321 gcgcctcgcg ccccttgcgg gcggggtcgc cgaccgcgag gcggagggtc ttgtcctggg
     1381 cggcggcggg ggcggccccg cagaggacgg cgagggcgag gagggcggac gcggcgagcc
     1441 tggtcttcat ggccggccta cgctaccgcg atgcggcagc ggaggcaacg gcccgcagcg
     1501 cctcctccag gaaggcgagc tgcaggcgca tctggtcgcg caggggcatg ccgcggacgc
     1561 cctggcgctc cgcctcggcg cggacctcct ccgcgtcccg cagggtggcc atcctttcga
     1621 gccaggcgcg gaagcgggga tcgtcgggcc ggccgctctc cccctcttcc agaggacgga
     1681 ggaagagggg catctcggag accagggtga aggggtcgcc gcccagggag cggacgtact
     1741 ccatggagct gggccggaag cgctcggcca tggccggatc ccccttctcc aggaagtagg
     1801 cgcgcatcgc caccgagtcg gggcgggtgg tgaagccttc gtcgatgcgg gagaaaccct
     1861 tctcgccgcc gcggtccacg tcgaagaccc gataccccag gtcgcgcacc cggcggcgca
     1921 ggctctcccg caagccggcg gtgcggtcgg tccaggcccg ctcgatgagg aaccaggggc
     1981 cggtggcgaa ggccatgccg tggaagctgc cgtggagggc gaacggcgcc ccttcagata
     2041 agaacgcggc cacggcgcgg ttctcgggcc gggcgccggc gtcctcttca tcgcgcggga
     2101 agccgaactc catgtcgtcc ccgggcggct cccgcatcac gtgccgcagg tagagcccga
     2161 ggtcatagac ccggtccgga gcgccccggc tgtccaccgc gggaaggggg tggccgctcc
     2221 aggcgctgtt gaccgcctca ccgtccggat tgacgtgggg gacgacgtac caggtgaagg
     2281 agatgagaag ggggtggtcc gccggcaggg ccgcgaggaa gccggcgagc cggcggagca
     2341 tctccggccc cacgggctcg tcggagtggc agccaccgat gagactgacg tggggcccgc
     2401 ccgttcccag gacgtgcccc acgatgtccc gaccctgccg tgaccggccg atcagccgcc
     2461 ccgatggagg cgcgatgccc gaggccagga tctcctcgat cgggagcgag aggattccca
     2521 tccctgtcta gcagttgaga caggagatgt agttcgtctg gccgcacatc gggcagttgg
     2581 tgtagtcccg cgtgcgctcc tggcagggca ggaaggtcgc gccgccggtg accgcctcga
     2641 ggttcatgcg gttcagggtc tccatattga ggtgcagctt cttgacgctc ttcttcttca
     2701 tggtcgattc ctttccgggg agtctcctcc cctcgagggt ggttcccatc tgcggagacc
     2761 acctaaatct accatgcgat tttcgccggc caatccggta acaacccacc cccgtccccc
     2821 gcctgtggtg tctaactgag gggaaacccg ggggcggagc gttcatcccg taaacaggag
     2881 gccggtgcgc tccgtatacc tagcaggagg gtgacgatgc aaatctcagc tttcaccgat
     2941 cgccacacac ccgaggagct cctgggaccg ctgaacgcca acgagctccg ccacgcgccc
     3001 gagcgcctct tcctggcggg cgacaccacc ctgctgtgga gcgcgccgca ggtggccgtc
     3061 atcggctcgc gcaacccgtc cgatctcggc cgctggcacg cggcgcgcct cgcccgcttc
     3121 ctgaccgacg aagggatcac catcgtcggc ggcctgtcga ccggcatcga gacggcggcc
     3181 cacgagacgg ctctccaggg cctggggcgc accatcgccg tgctcgccgc gccgctgggg
     3241 gtgccgccgt cgccgcgcaa cgccgagctg caccggcaga tcctggagcg gcacctggtg
     3301 gtctcgcagt tccccgccgg ccatccggtg acgcgcgagg acctgcggcg ccgcaactgc
     3361 accatggctc tcctctcgga cgccctggtc ctcgtcgagg ccgccgatgg cagcgcctcg
     3421 ctggaggtgg cctgggagat gctgcgcctg cgccggccgg tgttcatccc gcgcccggtg
     3481 gcggacgccg gctcgctgga gtggcccgag gagatgctgg ctcaaggcgc cagcgtcctc
     3541 gaccggcccg ccgccctcct cgccgccgcc gtccgcggct ccgcccgccg cttgagcgcg
     3601 accaccctcc gcctcgcctc ctgatcaccg gccgaatggc ccgcgggcag ggactctccc
     3661 ctgcccgtcc tggccctgcc cccacgctct agctttccct atagaatctc ccctggaggt
     3721 gtcccgtggc ggtctcggtc cgtaagcggc ttctgacgat cgacgagtac caccagatgg
     3781 tggagagcgg cattctcgcc gaaggcgaga gggtcgagct gatctacggg gagatcctgg
     3841 agatgaaccc gatcggccac cggcacgcca cctgcctgag gcgcctcatc cgcctcttcg
     3901 gccccggtgt cgtttccgac agtgtcgctc ccgacgtcct tctggacgta cagaaccccc
     3961 tccgcctccc caacgagcag tccgagcccc agcccgatct gatgctgctc cgcgggcggg
     4021 acgactgcta cgcctccaag ccccctgccg ccgaagacgt gctgctcctg gtcgagatcg
     4081 ccgaccactc cctggcctac gaccgggacg tcaagctgcc gctctatgcc caggcaggca
     4141 tccccgaggt ctggctcgtg gacctgacct cggacaccat ccgggtccac cgccggccct
     4201 ctcccgaagg ctacctggag gcccgccagg cgggccgtgg cgaggccatc gccccccaag
     4261 cgctcccccg tctgctggtg agagtcgagt tggtcctcgg ctgatcgatc cctcagccgc
     4321 cgcctacgaa gcgcctgaac aggcggtccg cgacatcctc cagatagctc cgcttctcga
     4381 ccttcaccgg cggcaggcct gccgacccgt tcgccgcccc ccagagcgca ccggccatgg
     4441 cgccgatcgt gtccacgtcc cctccgcagg cgatgacgaa ggccgtcatc tcctcgaaag
     4501 g
//
LOCUS       DASPJI010000436         4049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_256583, whole genome shotgun sequence.
ACCESSION   DASPJI010000436 DASPJI010000000
VERSION     DASPJI010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256583"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VEL74_11815"
     CDS             complement(<1..624)
                     /locus_tag="VEL74_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HYG63260.1"
                     /translation="MSKTPPPANARRSIRRHTAIGLASAAFLVGGLGGWAVVTELSGA
                     VVAPGQVVVDSHVKKVQHPTGGVVGEILVRDGDRVRAGDVVVRLDETVAKANLAMIDK
                     SLDELGVRQARLEAERDGLAAVVFPAGLLDRMAEAEVAGLLEGEQRLFATRREARAGQ
                     QAQLKERIAQLEEQIDGLSLQAQAKADEIKLIQDELTGVQELWKKNLV"
     gene            complement(684..2411)
                     /locus_tag="VEL74_11820"
     CDS             complement(684..2411)
                     /locus_tag="VEL74_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HYG63261.1"
                     /translation="MSAPSSPLRLLASCRGAVLGVALLSGLINILYLSGSIFMMEVYD
                     RVLPSRSIPTLVGLSLIVVVLYLFQGLFDALRGRILARIGAAVDEDLSQSVFQHQLAA
                     PLKGRPEGDGQQPLRDLDQIRAFLSGGGPSALFDLPWMPLYLFICFAFHPLIGLVTLA
                     GAACLVVITLLTEVLTRSAAREAVGAALVRNGLGEGARRNAEVVRALGMAGRLGARWG
                     EANARYLAYQQRTSDVAGGLGALSKVLRMLLQSAVLGVGAYLVIDGQATGGIMIASSI
                     LTSRALAPVELAIANWKGFVSARQGWARLRTQLAAAPAVTPLRLPAPKALLVVDNAGA
                     GAPGTQRFTVQDVSFQLAAGAGLGVIGPSASGKSSLARLLVGVWPAWRGKVRLDAAAL
                     EQWRPEDLGEHIGYLPQDVELFAGTVAQNIARFEAQPDPQAVIAAAQAANVHEMILHL
                     PDGYETAVGEAGAALSGGQRQRLGLARALYGNPFLVVLDEPNSNLDAEGEQALTQAIL
                     GVRARGGVVVVIAHRPSALAGVDQVLVMAEGRMQSFGPKDEVLGRVLRPTAVGPEPRL
                     PAAAPFKVA"
     gene            complement(2747..3481)
                     /locus_tag="VEL74_11825"
     CDS             complement(2747..3481)
                     /locus_tag="VEL74_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HYG63262.1"
                     /translation="MGEPPVAGLECHGGGGHDRLWGGAGDDRMWGGGGHDRFHGGQGA
                     DLMWGGRGNDTFYVDQAGDRVHESRGQGTDAVHASVSYSLAGTHVEKLVLTGAADLSA
                     TGNSLANTLTGNSGHNLLKGGAGHDVLKGNAGNDQLHGGSGHDRLNGGSGDDLLKGGS
                     GHDRLTGGSGRDTFVFEKRGGHDTVTDFRHGHDRIDVSQLAGVDGLGDLARVQVGRDT
                     MIWHNQDVLVLKGVSAAELDQSDFIF"
BASE COUNT          623 a         1423 c         1429 g          574 t
ORIGIN      
        1 caccaggttc ttcttccaca gctcctgcac ccccgtgagc tcgtcctgga tcagcttgat
       61 ctcgtccgcc ttggcctgcg cctgcaggct cagcccgtcg atctgctcct ccagctgcgc
      121 gatccgctcc ttgagctgcg cctgctggcc ggcgcgcgcc tcgcgccggg tggcaaacag
      181 ccgctgctcg ccctccagaa gcccggccac ctccgcctcg gccatgcggt cgagcaggcc
      241 ggccgggaac accaccgccg ccagcccgtc ccgctccgcc tccagccgcg cctggcgcac
      301 gccgagctcg tccaggctct tgtcgatcat ggccaggttg gccttggcca cggtctcgtc
      361 caggcgcacc accacgtccc cggcccgcac ccggtcgccg tcgcgcacca ggatctcgcc
      421 caccaccccg ccggtggggt gctgcacctt cttgacgtgg ctgtccacca ccacctggcc
      481 cggcgccacc acggccccgg acagctcggt caccaccgcc cagccgccca ggccgccgac
      541 cagaaaggca gcagacgcca ggccgatcgc cgtgtggcgg cggatcgagc ggcgggcgtt
      601 ggcgggcggc ggggtcttgc tcatgggatc ggtccttgac gggctcgggc gacggtatgg
      661 gtacggcgat gttgtggttg ggctcaggcg accttgaacg gggcggcggc cggcagccgc
      721 ggctcgggcc ccaccgcggt gggccgcagc acccggccca gcacctcgtc cttgggccca
      781 aagctctgca tgcgcccctc ggccatcacc agcacctggt cgaccccggc cagcgcgctc
      841 ggccggtggg cgatcaccac caccaccccg ccccgggccc gcacccccag gatcgcctgg
      901 gtgagcgcct gctcgccctc cgcgtccagg ttggagttgg gctcgtccag caccaccagg
      961 aacgggttgc cgtacagcgc gcgcgccagg ccgaggcgct ggcgctggcc gccggagagc
     1021 gccgccccgg cctcgcccac ggcggtctcg tagccgtcgg gcaggtgcag gatcatctcg
     1081 tgcacgttgg cggcttgcgc cgcggcgatc accgcctgcg ggtcgggctg agcctcgaag
     1141 cgggcgatgt tctgcgccac cgtgccggca aacagctcca cgtcctgcgg caggtagccg
     1201 atgtgctcgc ccaggtcctc cggccgccac tgctccagcg ccgccgcatc gagccgcacc
     1261 ttgccgcgcc aggccggcca caccccgacc agaagccgcg cgagcgacga cttgcccgag
     1321 gcgctcgggc cgatgacgcc caggccggct ccggcggcga gctggaagct cacgtcctgc
     1381 acggtgaagc gctgggtgcc gggcgcgccg gcgccggcat tgtccaccac cagcagcgcc
     1441 ttgggcgccg gcaggcgcag gggcgtgacc gccggggcgg cggccagctg cgtcctgagc
     1501 cgggcccagc cctggcgggc ggacacgaag cccttccagt tggcgatcgc cagctcgacc
     1561 ggggcgagcg cccgggaggt gaggatcgag gaggcgatca tgatgccgcc tgtggcctgg
     1621 ccgtcgatga ccagataggc gcccaccccc agcaccgccg attgcagcag catgcgcagc
     1681 accttggaca gggcgcccag gcccccggcc acgtcggagg tgcgctgctg gtaggcgaga
     1741 tagcgggcgt tggcctcgcc ccagcgggcg ccgaggcgcc cggccatgcc cagggcgcgc
     1801 accacctcgg cgttgcgccg cgcgccctcg cccaggccgt tgcgcaccag cgccgcgccc
     1861 accgcctcgc gggcggcgga gcgggtcagc acctcggtga gcagggtgat caccaccagg
     1921 caggcggcgc cggccagcgt caccaggccg atgagcgggt ggaaggcaaa gcagatgaac
     1981 aggtagagcg gcatccaggg caggtcgaac agggccgagg ggccgccgcc ggacaaaaag
     2041 gcgcggatct ggtcgagatc gcgcaggggc tgctggccgt cgccctcggg ccggcccttg
     2101 agcggggcgg cgagctggtg ctggaacacg ctctggctca ggtcctcgtc cacggcggcg
     2161 ccgatgcggg ccaggatgcg cccgcgcagg gcgtcgaaca ggccctggaa caggtagagc
     2221 accaccacga tcagcgacag gccgaccagg gtggggatgg agcggctggg cagcacccgg
     2281 tcgtagacct ccatcatgaa gatcgagccg gacaggtaca ggatgttgat caggccgctg
     2341 agcagggcca cgccgagcac ggccccgcgg cacgaggcca gcagacgaag aggggaagac
     2401 ggagcgctca tgggtgcctc gggcagggtg ccttgggaag ggggccgtga gaaggacgcg
     2461 gaggaagcca tcctggcctg gaaccgccgg ggccgatagc gtcgaagggc tgatgcgggc
     2521 tggggctaag gtctgatggg cccaaggtcc gagggtcgaa gcaggcgggg ccaggaacac
     2581 agggtccaaa gaacacaggg tccaagaaca cagggtccaa gaacacaagg tccaaggatg
     2641 cccatcgagg gatgcgggtc gaaggggtcg tccgccgggg gctgtggggg gctttggcct
     2701 ccggcggagg agatgcggcc cgggggccgc atcgaggggt gggggatcag aagatgaagt
     2761 cggactgatc cagctcggcg gcggagacgc ccttgagcac cagcacatcc tggttgtgcc
     2821 agatcatcgt gtcgcgcccg acctgaaccc gggccagatc gcccaggccg tcgacgccgg
     2881 caagctggct gacgtcgatg cggtcgtggc cgtggcggaa gtcggtgacg gtgtcgtggc
     2941 cgccgcgctt ctcgaacacg aaggtgtcgc gcccggagcc gccggtgaga cggtcatgac
     3001 cggagccgcc cttgagcagg tcgtcgccgg agccgccgtt gaggcggtca tggcccgagc
     3061 cgccgtggag ctggtcgttt ccggcgttgc ccttgaggac gtcgtggccg gccccgccct
     3121 tgagcaggtt gtggccgctg ttgcccgtca gggtgttggc caggctgttg ccggtggcgg
     3181 acagatcggc cgcgccggtg aggaccagct tctccacgtg ggtgccggcg agcgagtagg
     3241 acacgctggc gtggacggca tcagtgccct ggccgcggct ctcatggacg cggtcgccgg
     3301 cctgatcgac gtagaaggtg tcgttgccgc gcccgcccca catgaggtcg gcgccctggc
     3361 cgccgtggaa gcggtcgtgg ccgcccccgc cccacatgcg gtcgtcgcct gccccgcccc
     3421 agaggcggtc gtggccgccc ccgccatggc actccagccc cgccaccgga ggctcaccca
     3481 ccggaggtgc cggaggctga tccaccggag gctgagacac aggagggtca tcgtccgtat
     3541 caggaagatc tcggacgttt atcgttatgc tcttggtgta catcaggcca tgtgaatccc
     3601 tcgcagtaac cagaaggtca tgtgctgcgg cattctcgaa atcgatcaca gccccttgcc
     3661 taaccttgat ctgctcaccg tccacctcaa acagtgctgc tgatggaccg tccaaggata
     3721 gaatgggggt atctccggag tctggatcaa tcgtcttaag cttagcgacc acagtgccgg
     3781 cgacagcatt ttccagcaca acccctcccc cgacgatcac gatatcgatc ggagcctcgt
     3841 tcacgttggt cagcgccagg gtatagtact cgcttgacgt atggccgccg accgtcgccg
     3901 tgatggaaac tgcgtaggca ttcttgcttt cgtagtcgag agcggtcccg gctttcaggt
     3961 aaagggcgtt gccgtcgatc tcgaatgccc cagcgtcagc gcccgacaga gcaagcgctc
     4021 cagctattcc agccccggcg atatccgca
//
LOCUS       DASPJI010000437         3090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_596032, whole genome shotgun sequence.
ACCESSION   DASPJI010000437 DASPJI010000000
VERSION     DASPJI010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596032"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VEL74_11830"
     CDS             complement(<1..603)
                     /locus_tag="VEL74_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63263.1"
                     /translation="MRKLCCVLALAGLLGFGGQAFAVINTIDDVPGATLLLPYFEVDL
                     NDPQGVTTLFSINNASATAVLAHVTLWTDQSVPAIDFDVYLTGYDVQTINLRDIFVNG
                     TLPVTASAGQDPGDAISPQGNVSQDINFASCTGVLPYANPAVSASFRAHLQAWFTGNQ
                     SPATGNCAGARIRDGIARGYVTIDTVNACNLLFPSSPGYTA"
     gene            complement(809..1426)
                     /locus_tag="VEL74_11835"
     CDS             complement(809..1426)
                     /locus_tag="VEL74_11835"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HYG63264.1"
                     /translation="MNPRRPLRWLLWSLLVVALVAVAAAAAVQWLRNPDAPPVMGQVP
                     DFSLTNRDGSTVRLADLAGAPWIADFVFTRCNGSCPLMSLRMQRLNRELPPDLGVRLV
                     SITVDPDYDTPQILQAYAESFSAPGRWLFLTGAKEEVYGLIRQGFKLALDPSPPPGAA
                     TAEEPIIHSTRFVLVDGRGQIRGYYGAFDEEAMARLAEDLRAIAD"
     gene            complement(1423..2184)
                     /locus_tag="VEL74_11840"
     CDS             complement(1423..2184)
                     /locus_tag="VEL74_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG63265.1"
                     /translation="MSSTALLATGTLWRRELVRFFRQPSRIAGAAGSPLLFWILIGSG
                     LSGSFRLPGGPSDVDFLEYFFPGTVVLVLLFAAIFSTISVIEDRQQGFLQGVLVAPVP
                     RAAVVAGKVLGGATLAWLQALVFLALAPLSGIRLTLLSALAAAGVMALLAVGLTAIGF
                     VFAWRVDSVQGFHAVMNLLLVPMWLLSGAFFPLAGAPGWLAALMSVNPLTYGVSALRG
                     ALYGAVPPPAAGVTAAVALAAFAASVLATRGGRVE"
     gene            complement(2181..>3090)
                     /locus_tag="VEL74_11845"
     CDS             complement(2181..>3090)
                     /locus_tag="VEL74_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYG63266.1"
                     /translation="RRGGARKALAGVGFQVAAGEVFGFLGPNGGGKSTLFRILATLAR
                     PERGEVRIFGIDPARQVREVRRLLGVVFQSPGLDLQLTVGENLLHHGHLYGLRGRALA
                     RAIDEALARFGLADRREERARTLSGGLRRRVEIAKALLHGPRLLLLDEPSTGLDPGAR
                     RDLWDTLESLRAGGVTVLLTTHSMEEADRCDRLALLDRGSLVAEGTPAALKEEVGGDV
                     VTLTGRDPESLARDLAARFPGLAPAVWEGAVQITRERAHELVARLVEALPGRIDSVTV
                     ARPSLEDVFLRRTGHRLYGGEGEAEA"
BASE COUNT          497 a         1061 c         1167 g          365 t
ORIGIN      
        1 ggcggtgtag cccggcgacg acgggaacag caggttgcag gcgttgaccg tgtcgatcgt
       61 cacgtagccg cgggcgatgc cgtcgcggat ccgggcgccg gcgcagttgc cggtggccgg
      121 cgactggttg ccggtgaacc acgcctggag gtgggcgcgg aacgaagcgc tgaccgccgg
      181 gttggcgtac ggcagcacgc ccgtgcacga ggcgaagttg atgtcctgcg acacgttgcc
      241 ctgggggctg atggcgtcac ccgggtcctg gccggcggag gccgtgaccg gcagggtgcc
      301 gttgacgaag atgtcgcgca ggttgatcgt ctgcacgtca tagcccgtca ggtagacgtc
      361 gaagtcgatg gccggcaccg actggtcggt ccacagggtg acgtgggcca gcaccgcggt
      421 ggccgacgcg ttgttgatgg aaaagagcgt ggtgacccct tgcggatcgt tgaggtcgac
      481 ctcgaagtag gggagcagca gggtggcgcc gggcacgtcg tcgatcgtgt tgatgacggc
      541 gaaggcctgg ccaccgaacc ccagcagacc cgccagagcc agtacgcagc aaagctttct
      601 catcggactg atctcctcac agagactacg gtttctcgct tccagcagtc gggctcccct
      661 cagtggaagc cggcccgccg ggcggtggag caaaagggaa gagacgtgat gacgtctctg
      721 tacaacgaat gcttgcgggg actataacgt ttcgtggcga ccggtacaac tacccggcgt
      781 gcaggcggtg ccctccgccg ccgccgggct agtccgcgat cgcccgcagg tcctcggcga
      841 gcctggccat cgcctcctcg tcgaaggcac cataatagcc gcggatctgg ccgcggccgt
      901 ccaccagcac gaagcgggtg ctatgaatga tgggctcctc ggcggtggcg gcgccggggg
      961 gcggcgaggg atcgagcgcc agcttgaagc cctgccggat caggccgtag acctcctcct
     1021 tggcaccggt caggaagagc caccggccgg gggccgagaa ggattccgcg taggcctgga
     1081 ggatctgggg cgtgtcgtag tccgggtcga cggtgatgga gacgagccgg acgccgaggt
     1141 ccggcggcag ctcccggttc agccgctgca tgcgcaggct catcagcgga caggagccgt
     1201 tgcagcgggt gaagacgaag tcggcgatcc acggcgcgcc ggccaggtcc gcgagccgga
     1261 ccgtgctgcc gtcgcggttg gtgaggctga aatccggcac ctgtcccatg accggcggcg
     1321 cgtcagggtt gcgcagccac tgcacggcgg cggccgcggc caccgccacc agcgccacca
     1381 cgagcaggct ccagaggagc catcggaggg ggcgtcgcgg attcactcca ccctcccccc
     1441 gcgggtcgcc agaacgctcg cggcgaaagc tgccagggcg acggcggcgg tcacgcccgc
     1501 ggccggcggc ggcacggcgc cgtacaacgc gccgcgcagc gccgacacac cgtaggtcaa
     1561 cgggttcacg gacatcagcg cggcgagcca gccgggcgcc cccgccagcg ggaagaaggc
     1621 gcccgagagg agccacatgg gcaccagcag gaggttcatc accgcgtgga acccctggac
     1681 cgagtccacc cgccaggcga agacgaagcc gatggcggtc aggcccacgg cgagaagggc
     1741 catgacgccg gccgccgcca gcgccgacag aagcgtcagc cggatgcccg agagcggcgc
     1801 cagggcgagg aagaccaggg cctggagcca ggcgagcgtc gccccgccca gcaccttccc
     1861 cgccaccacg gccgcccgcg gcaccggcgc caccagcacc ccctggagga acccctgctg
     1921 gcggtcctcg atcaccgaga tggtcgagaa gatggcggcg aagagcagca ccagcaccac
     1981 ggtgccgggg aagaagtact ccaggaagtc gacgtcggac ggccctcccg gcagccggaa
     2041 cgagccggag aggccgctgc cgatgaggat ccagaagagg aggggcgagc cggccgcgcc
     2101 cgcgatgcgg ctgggctgcc ggaagaagcg caccagctcg cgccgccaga gggtgcccgt
     2161 cgccaggagc gcggtggagc tcacgcctcg gcctcccctt ccccgccgta cagacggtga
     2221 cccgtgcggc gcaggaagac gtcctcgagg ctcggccggg cgacggtgac cgagtcgatc
     2281 cgccccggga gcgcctcgac gaggcgggcc accagctcgt gggcacgctc gcgggtgatc
     2341 tgcaccgcgc cctcccatac cgccggcgcg aggccgggga agcgggccgc caggtcgcgg
     2401 gcgagcgact cggggtcgcg gccggtgagg gtgaccacgt cccctcccac ctcctctttc
     2461 agcgccgccg gcgtcccctc ggccaccagg gagccacggt cgaggagggc gagccggtcg
     2521 cagcgatccg cctcctccat cgaatgggtg gtgaggagca cggtgacgcc gccggcgcgg
     2581 agcgactcca gcgtgtccca caggtcgcgc cgggcgccgg ggtcgagccc ggtgctgggc
     2641 tcgtccagca ggagcaggcg cgggccgtgg agcagggcct tggcgatctc gacgcgccgg
     2701 cgcaggccgc cggagagggt gcgggcgcgc tcctcccgcc ggtccgcgag gccgaagcgg
     2761 gccagcgcct cgtcgatcgc ccgggcgagg gcccggccgc gcagcccata gagatgtcca
     2821 tggtgcagga ggttctcgcc gacggtgagc tggaggtcca ggccggggct ctggaagacc
     2881 acgccgagca ggcgccgcac ctcacgcacc tggcgggccg ggtcgatgcc gaagatccgg
     2941 acctcgcccc gctccggccg ggccagggtg gcgaggatgc ggaacagggt gctcttgccg
     3001 ccgccgttgg ggccgaggaa gccgaagacc tcgccggcgg ccacctggaa gccgacgccg
     3061 gccagggcct tgcgggcgcc cccccggcgg
//
LOCUS       DASPJI010000438        13136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_372381, whole genome shotgun sequence.
ACCESSION   DASPJI010000438 DASPJI010000000
VERSION     DASPJI010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13136)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13136
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372381"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1392
                     /locus_tag="VEL74_11850"
     CDS             100..1392
                     /locus_tag="VEL74_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HYG63267.1"
                     /translation="MNRRVAILLTLAALVLAGLGLWIRFRSGDGEDLAGILGEIREPA
                     EDRLELRPARFADLPGWGEDAIAEALPALLRSCGRIANLPDGASMAPRQEGLGFAGTA
                     ADWKPACAEAAGLPAGDHAAARAFFERRFRPMAATNNGHPLGLFTGYYEPLLQGSRTR
                     GGRFTVPLYLRPPELVTVDLGDFRESLKGQRIAGRVEDGALVPFPDRDAIEEGALAGR
                     RLELVWVDSPVDAFFLHVQGSGRVRLAEGGEMRVGYAAQNGHPYFAIGRDLIERGALE
                     QEEVSMQSIRAWLEAHPEEASEVMARNASYVFFQELEGDGPLGAEGVPLTPGRSLAVD
                     LRFMPLGLPVWLSAGMPGAREGDPDRKLRRLLVAQDTGGAIRGPVRGDVFWGYGEEAA
                     EIAGRMKHRGRLWVLVPAVSEPRVPAGRPASSAPAPPG"
     gene            complement(1331..2158)
                     /locus_tag="VEL74_11855"
     CDS             complement(1331..2158)
                     /locus_tag="VEL74_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63268.1"
                     /translation="MYNRNPAGPAGRSTLAGIGGFGGPVPRDLLVILGVVFATFALQF
                     FDATRGIVALLRLTPLVWQSGFVWQLATYPFIGYGGPSIWFLLELLILFWFGRDVFAG
                     LRRRHFWRMLVWSTVGSALVAVAVQLLAGLTTGGVGMADFTLMQGQRMLMAIFIAAFA
                     TANRDATILLFFILPIQARWFLALEILFAFMGFLATKDLPGFLGICAAVGLTWGYIKA
                     SGTMKGGGRTLRETRLRLERWWIQRKLERNRKKRGFRVIQGEREQKTPDGRRGPWVH"
     gene            complement(2162..3469)
                     /locus_tag="VEL74_11860"
     CDS             complement(2162..3469)
                     /locus_tag="VEL74_11860"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG63269.1"
                     /translation="MRLENIGVVAKREYLLRVKSKGFWIGTLVLPLFIVALSVLPTLF
                     LTKSRTDHQVVVVDETGRIGQEFAANPNGAQPQKEGEEGKDQRERVARFEVEVEAPAA
                     DREAQRAALDRRVLAEEIDSWVWIGEGVLRGDPVEYHARSVSNFMTQEVLEDRLSELV
                     RRTRLTEAGYDPDRVGELSGSIDLKTVRISEEGSRAEGGLGSVAFAYILFFLLYTMMM
                     LWGQQVMNGVLEEKGSRIVEVVLSSIQPFELMMGKLTGICLAGLTQFVIWLGTMMLVT
                     APGMLAAMATLPEELVLPTVTLGMVLNYILLFVLGFFVYATMYAAIGSAFNNLQEAQQ
                     VASVAVFFLVVPFMIMFQIINDPNSTLAVVTSLIPPFTPMLMTLRVSLDMPPLWQLLL
                     GYVLTIAFIWGMVWVCARIYRVGILMYGKKPTLQEIWRWVRYA"
     gene            complement(3459..4370)
                     /locus_tag="VEL74_11865"
     CDS             complement(3459..4370)
                     /locus_tag="VEL74_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYG63270.1"
                     /translation="MNPMTPTLELREVRKRYGDFAAVDGVSLAVPPGTIYGLLGPNGA
                     GKTSTIRMIMNITAPDSGEVHLFGHPRRPEDLRRIGYLPEERGLYRKMTVIDQLLFLG
                     EIRGLKRRELLPRIERWLERVGLAEWGKKKVEELSKGMQQKVQLVGTVLHEPDLLILD
                     EPFSGLDPLNQELFKELLVDYKQQGKSVVLSTHGMELAERMCEHICLIFKGRAVLAGE
                     LRQIKREVAGNTYRLVADGDLDRLPQVPGVEQASVQNGVVKLMLRPEAEGPEVLREMV
                     QFLRVHEFRSEEPELEQIFLKAVRDAA"
     gene            complement(4437..7187)
                     /locus_tag="VEL74_11870"
     CDS             complement(4437..7187)
                     /locus_tag="VEL74_11870"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYG63271.1"
                     /translation="MIDPERWQEVDRVFAEALERAPAERPAFLDAACAGDAALRRGVE
                     RLLAADQAGTDFLESPPGELLRLALDDREEGGRLGPYRLLRRIGRGGMGTVYLARRED
                     EHYQRDVAVKVLRASLASTEAYHRFLAERQILAHLEHPNIARLYDGGSTEDGRPYLVM
                     ERVEGMPVDLYCDHHRLTVDQRLVLFQKICAAVQYAHQSLLVHRDLKPGNILVTADGE
                     PKLLDFGIAKRLAQEKDALDETWDGLRMLTPSYASPEQVRGEAITTASDVYSLGVILY
                     ELLAGRRPCRAPGDQPQDIERAVCEQEPEPPSAALVRPGPSDVDETARARRTRPRALA
                     RRLEGDLDGIALMALRKDPRQRYGSAAQLAQDLERHLRGRPVTARPPTVRYRTGKFIR
                     RHRAGIASAALVVLIVAAFLASLVAQGRQLARERDKARSTLSFLVDTFKQADPYQTEG
                     ERLTARQILDQGAERSARELSGQPDVQAAVMDTIGEVNLGLGRYDAAEPLLERALALR
                     REAFGPESLEVAESLEHLAALQGERADRAGAESRLRQALAIRRLHQGDDHLAVARTLN
                     RLGEVLVARGVSPEESAEIEALHQEALTIARRVEGPAGLTVSETLLALSDLQRAQGDY
                     AAAERLFREGLAIERKVFGERAPRLWRDQSRLAQLLLEAGKLKEAEALSRRCLEVQGQ
                     ILDRGHADIVETSSVLAMAVHNQGRYVEAEALHRRVLALSRAHYGPGHWQLSMALGNL
                     AANLDAQGRGPEAIPLYEEALEIRRRKLGDGDPLVAQILLLLAELHRNDESYPRGLEL
                     ARQALEILEKAEGPDHPHVAFALREIGRNYLIQRRFTEAEPFLRRSLEIRRRALEPRH
                     PDLAKAQVSYSRCLIGLGRDREAVPLLREARTTFAAVYGPAHEMTRTPEDLLEEIEGR
                     TP"
     gene            complement(7213..7794)
                     /locus_tag="VEL74_11875"
     CDS             complement(7213..7794)
                     /locus_tag="VEL74_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG63272.1"
                     /translation="MPLPEGTEATITLLLKGWSSGDQEAAERVLPLVYEELRHIAVRQ
                     LRQERDAHTLQATAIVHEAYLRLSGQAGLEWPSRAHFFGFAAHLIRRVLVDYARYRNR
                     AKRGGRIEKVSLAEVAELALEKGTDLLALDEALSALEKVDPRKAAVVELKFFAGLSLD
                     EVAEQLGISAETVSRDWRRAKAWLYDALQSGAS"
     gene            8254..8634
                     /locus_tag="VEL74_11880"
     CDS             8254..8634
                     /locus_tag="VEL74_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63273.1"
                     /translation="MSKEVTYAGKRGAWRQRLNTVAANAGDLEHLQGLANRLEATLQA
                     VEGVARQQAIFIAGKQESSQQLAALMGDGERLDTLLRKGLQQHYGIGSEKLAEFGVQP
                     YRGRTRKAVLPVEDTSPPSADPAE"
     gene            9247..10563
                     /locus_tag="VEL74_11885"
     CDS             9247..10563
                     /locus_tag="VEL74_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008017889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HYG63274.1"
                     /translation="MFIAKKSIVVLAVVAVGLSGCASSRLPVSATTGPDPELPPPNKS
                     LIPVVNVVKAIGWPEGVTPAAAEGTAVAAFARDLDHPRWLYVLPNGDVLVAETNAPPR
                     PEDSKGIKGFFFKLFQKKAGGAVPSANRITLLRDTDGDGTAETRTAFLSGLNSPFGME
                     LVGDVLYVANSDAVVKFPYTQGATQLTAPAVKVVDLPAGPRNHHWTKNVIASRDGSKL
                     YVAIGSNSNVAEHGMAEEEGRAAIWEVDAATGSHRVFASGLRNPVGMAWEPESGALWT
                     AVNERDELGNDLVPDYMTSVRDGGFYGWPYSYYGQNVDRRVKPPRPDLVAKALVPDYA
                     LGPHTASLGLAHSQGTSLPDRFGRGMFVGQHGSWNRKPRSGYKVIFVPFSGSRPAGQP
                     LDVLTGFVKENGEAMGRPVGVAVDKKGALLVADDVGNVIWRVTSAR"
     gene            complement(10547..12139)
                     /locus_tag="VEL74_11890"
     CDS             complement(10547..12139)
                     /locus_tag="VEL74_11890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG63275.1"
                     /translation="MSDQDGKPSPPQPSLLEESAEDLYENAPCGYLSALPGGMILKVN
                     QTFLAWTGYRREDLAGRKRFYELLTAGGRIFHETHYAPLLQMQGTVRELALDLVCADG
                     HRLPVLINSVLRKDEDEKPLLIRTTVFNATDRKEYERELLRERQRAEQASQTKSDFLA
                     MISHEIRTPLNAITGVAHLLGQTDLSPQQQKLVRILGSSSGNLLHLVNQILDFGKIEA
                     GKMSLEERSVDLRQLVREVAESFEPRAQAKGLALLVHVDERVPRTVAGDPMKLGQVLS
                     NLAGNAIKFTSEGSVILALGVRTIEPEAVSIDFRVSDTGIGIAPDRLARIFDDFTQAN
                     YDIGLTYGGTGLGLSISRKLVEMHGSRILVASEPGKGTTFSFELRFKTVAEAETAPAQ
                     ALVTSGLEGLEVLVADDNEVNVFVLTGLLKGWGVAFDVVSNGRQAVERVRARDYDLVL
                     MDLRMPELDGYAATREIRSLPGERFAKLPIFALSASTRMAHQHEIDAAGFNEFVGKPI
                     SPDVLFGKMARYAPARHATAPR"
     gene            complement(12155..12946)
                     /locus_tag="VEL74_11895"
     CDS             complement(12155..12946)
                     /locus_tag="VEL74_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYG63276.1"
                     /translation="MNVLQRNNVQLSGRATGQPMVFAHGFGCDQNMWRFVTPAFEDEY
                     RIVLFDHVGAGRSDLSAYNQAKYSSLQGYASDVLEICRELDLTRVVFVGHSVSAAIGV
                     LAANEEPERFDALVLVGPSPCYIDDGDYVGGFSREDIEGLLESLDSNYLGWSSAMAPV
                     IMGNPDRPELGTELTNSFCRTDPAIARHFARVTFLSDNRADFPKVKVRSLVLQCSEDA
                     IAPRCVGEYVHRNIPGSELVLMNATGHCPNLSAPEETIAAIQRFL"
     gene            complement(12943..>13136)
                     /locus_tag="VEL74_11900"
     CDS             complement(12943..>13136)
                     /locus_tag="VEL74_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63277.1"
                     /translation="KPLISSAREEIAAARASLSLSIEGAAYQPPDPDVTETLSRGNVL
                     SSCRRKSPGGDARHGIMGP"
BASE COUNT         2006 a         4430 c         4644 g         2056 t
ORIGIN      
        1 ttccggggct gccccggctg cggccgcgtc tattggagag ggacccacgt cgaccggatg
       61 gggcgctggc tcgagcgggc cggagtggag ctgtaggcga tgaaccgccg cgtcgccatc
      121 ctgctgaccc tggccgccct ggtgctcgcc ggcctcggcc tctggatccg cttccggtcc
      181 ggggacgggg aggatctggc cggcatcctg ggggagatcc gcgagccggc cgaggaccgg
      241 ctggagctga ggccggcccg cttcgccgac ctcccgggtt ggggggagga cgcgatcgcg
      301 gaggccctgc ccgccttgct ccgctcctgc gggcggattg ccaacctgcc cgatggcgca
      361 tccatggctc cccgtcagga ggggctcggg ttcgccggca cggcggcgga ctggaagccg
      421 gcctgcgcgg aggcggccgg gctgccggcc ggggaccacg cagcggcgcg cgccttcttc
      481 gagcgccggt tccggcccat ggcggccacc aacaacggcc accccctggg cctcttcacc
      541 ggctactacg agccgctcct ccagggcagc cgcacgcggg ggggccgctt caccgtcccc
      601 ctctacctgc ggcccccgga gctggtgacg gtcgacctcg gggatttccg cgagtcgctg
      661 aaagggcagc gcatcgccgg cagggtggag gacggcgccc tggtcccctt cccggaccgc
      721 gacgccatcg aggagggggc cctggccggc cggaggctcg agctggtctg ggtggacagt
      781 ccggtggacg ccttcttcct ccacgtccag ggctcggggc gggtccgctt ggccgaaggg
      841 ggggagatgc gggtgggcta cgcggcgcag aacggccacc cctacttcgc catcggccgc
      901 gacctcatcg agcgcggcgc cctggagcag gaggaggtct ccatgcagtc gatccgcgcc
      961 tggctcgagg cccatccgga ggaggcctcg gaggtgatgg cgcggaacgc ctcctatgtc
     1021 ttcttccagg agctcgaagg ggacgggccg ctgggcgccg agggggtgcc gctgacgccc
     1081 ggacggtcgc tggcggtgga cctgagattc atgcccctgg gattgccggt ctggctctcc
     1141 gccggcatgc cgggagcccg ggagggggac cccgaccgca agctccgccg ccttctggtc
     1201 gcccaggaca ccggtggcgc catccgcggg ccggtgcgcg gcgatgtctt ctgggggtac
     1261 ggcgaggagg ccgcggagat cgccggccgg atgaagcacc gcggccggct ctgggtgctg
     1321 gtgccggccg tcagtgaacc cagggtcccc gccggccgtc cggcgtcttc tgctcccgct
     1381 ccccctggat gacccggaag ccgcgcttct tgcggttccg ctccagcttg cgctggatcc
     1441 accagcgctc cagccgcagc cgcgtctcgc gcagagtcct ccccccgccc ttcatcgtgc
     1501 cgctcgcctt gatgtagccc caggtcagcc ccaccgcggc gcagatgccg aggaagccgg
     1561 ggaggtcctt ggtcgccagg aagcccatga aggcgaacag gatctccagg gcgaggaacc
     1621 agcgcgcctg gatggggagg atgaagaaga gcaggatcgt cgcgtcgcgg ttggcggtgg
     1681 cgaaggcggc gatgaagatc gccatcagca tccgctgccc ctgcatcagg gtgaagtcgg
     1741 ccatgccgac accacccgtg gtcagcccgg cgaggagctg gacggcgacc gcgaccagcg
     1801 ccgagcctac ggtgctccag acgagcatcc gccagaagtg gcggcgccgc aggccggcga
     1861 agacgtcacg gccgaaccag aagaggatca gcagctcgag caggaaccag atcgacgggc
     1921 cgccgtagcc gatgaacgga taggtggcga gctgccagac gaagccgctc tgccagacga
     1981 gcggcgtgag ccgcagcagg gcgacaatcc cgcgggtggc gtcgaagaac tgcagggcaa
     2041 aggtggcgaa caccaccccg aggatgacga ggaggtctct gggcaccggc ccgccgaagc
     2101 cgccgatgcc ggccagggtg gaacgtccag cggggccggc ggggttgcgg ttgtacatcg
     2161 gctaagcgta gcgcacccag cgccagatct cctggagcgt cggcttcttg ccgtacatga
     2221 ggatgccgac ccggtagatg cgggcgcaga cccagaccat gccccagatg aaggcgatgg
     2281 tgaggacata gccgaggagg agctgccaga gggggggcat gtccagggag acccggagcg
     2341 tcatcagcat cggcgtgaac ggcgggatca gcgacgtcac caccgccagc gtcgagttcg
     2401 ggtcgttgat gatctggaac atgatcatga acggcacgac caggaagaac accgcgacgc
     2461 tcgccacctg ctgcgcctcc tggaggttgt tgaaggcgga gccgatcgcg gcgtacatgg
     2521 tcgcgtagac gaagaagccg agcacgaaga ggaggatgta gttgagcacc atccccaggg
     2581 tgacggtggg gagcaccagc tcctcgggca gggtggccat ggcggcgagc ataccgggcg
     2641 ccgtcaccag catcatggtg ccgagccaga tgacgaactg ggtgagcccc gccaggcaga
     2701 tgccggtgag cttgcccatc atcagctcga acggctggat cgaggagagc accacctcga
     2761 cgatgcgcga gcccttctcc tcgagcacgc cgttcatcac ctgctggccc cagagcatca
     2821 tcatcgtgta gagcaggaag aagaggatat aggcgaaggc gacgctgccc agcccccctt
     2881 cggcgcggct cccctcctcg gagatccgca ccgtcttgag gtcgatggag ccggacagct
     2941 ctcccaccct gtcggggtcg tagccggcct cggtgagccg agtccgccgc accagctcgg
     3001 agagccggtc ctccagcacc tcctgggtca tgaagttgga gacgctgcgg gcgtggtact
     3061 ccaccgggtc gccgcgcagc accccttcgc cgatccagac ccaggagtcg atctcctcgg
     3121 cgaggacgcg gcggtcgagc gcggcgcgct gcgcctcgcg gtcggcggcc ggcgcctcga
     3181 cctccacctc gaaccgggcg acccgctccc gctggtcctt cccctcctcc ccttccttct
     3241 gcggctgcgc cccgttgggg ttggccgcga actcctggcc gatccggccg gtctcgtcca
     3301 ccaccaccac ctggtggtcg gtccgggact tggtgaggaa cagggtcggc agcacgctca
     3361 gggcgacgat gaacaggggg agcaccaggg tgccgatcca gaatcccttg ctcttgaccc
     3421 gcagcaggta ctcccgcttg gccaccactc cgatgttctc aagccgcatc gcgcaccgcc
     3481 ttcaggaaga tctgctcgag ctccggctcc tcggagcgga actcgtggac acgcaggaac
     3541 tggaccatct cgcgcagcac ctcgggcccc tcggcctcgg ggcgcagcat cagcttcacg
     3601 acaccgttct ggacgctcgc ctgctcgacg ccgggtacct gcggcaggcg gtcgaggtcg
     3661 ccgtcagcca ccagccggta ggtattgccg gcgacctcgc gcttgatctg ccggagctcg
     3721 ccggccagaa cggcccggcc cttgaagatc aggcagatgt gctcgcacat gcgctcggcc
     3781 agctccatgc cgtgggtgga gaggacgacg ctcttcccct gctgcttgta atccaccagc
     3841 agctccttga acagctcctg gttgagcgga tcgaggccgg agaagggctc gtcgaggatc
     3901 agcaggtcgg gctcgtggag caccgtgccc acgagctgca ccttctgctg catgcccttg
     3961 gacagctcct cgaccttctt cttgccccat tcggccagtc cgacccgctc gagccagcgc
     4021 tcgatgcggg ggaggagctc gcggcgcttc aggccgcgga tctcgcccag gaagaggagc
     4081 tggtcgatga ccgtcatctt ccggtagagg ccgcgctcct ccggcaggta gccgatccgc
     4141 cgcaggtcct cgggccggcg cgggtggccg aagaggtgca cttcgcccga atccggggcg
     4201 gtgatgttca tgatcatgcg gatggtcgac gtcttgccgg cgccgttggg tcccagcagg
     4261 ccgtagatgg tgccgggggg caccgccagc gagacgccgt cgaccgccgc gaagtcgccg
     4321 tatctcttac ggacctcgcg cagctccagg gtgggtgtca tggggttcat cgtagccgaa
     4381 tcctccgtgg aagatacggc gaagaggcgg gaaggtttgc ggaccggccc cgtggcctac
     4441 ggcgtccggc cctcgatctc ctccagcagg tcctcggggg tccgcgtcat ctcgtgcgcc
     4501 gggccataga cggcggcgaa cgtcgtccgc gcctcgcgca gcagcggcac cgcctccctg
     4561 tcgcggccga gcccgatcag gcagcgcgaa tacgagacct gcgccttggc caggtcgggg
     4621 tgccggggct ccagcgcccg gcgccggatc tccagcgagc ggcgcaggaa gggctcggcc
     4681 tcggtgaagc ggcgctggat caggtagttc ctgccgatct cccgcagcgc gaaggccacg
     4741 tgggggtggt ccggcccctc ggccttctcg aggatctcga gcgcctgccg ggcgagctcg
     4801 agccccctcg ggtaggactc gtcgttgcgg tggagctcgg cgaggagcag gaggatctgg
     4861 gccaccagcg ggtccccgtc gccgagcttc cggcgccgga tctccagcgc ctcctcatag
     4921 agagggatgg cttccggccc cctgccctgg gcgtcgaggt tggccgccag gttgcccagc
     4981 gccatggaca gctgccagtg cccgggaccg tagtgggcgc gggacagcgc cagcacccgc
     5041 cggtgcaggg cttcggcttc gacataccgg ccctgattgt ggaccgccat cgccaggacg
     5101 ctcgaggtct ccacgatgtc cgcatgcccg cggtcgagga tctgcccttg cacctccagg
     5161 cacctccggc tgagagcctc cgcctccttg agcttgcccg cctccaacag gagctgcgcg
     5221 agccgggact ggtcgcgcca gagccggggg gcccgctccc cgaagacctt cctctcgatc
     5281 gccagcccct cccggaagag acgctcggcc gcggcgtagt ctccctgggc ccgttgcagg
     5341 tcggagagcg cgaggagggt ctcggacacg gtgagccccg ccgggccctc cacgcggcgg
     5401 gcgatggtca gggcttcctg gtggagcgcc tcgatctccg cgctctcctc cggggagacg
     5461 ccccgggcga cgaggacctc gcccaggcgg ttgagcgtcc gcgccacggc gaggtgatcg
     5521 tccccctggt ggagacggcg gatcgccagc gcctggcgca ggcgcgactc cgccccggcc
     5581 cggtcggcgc gctcgccctg gagcgcggcc aggtgctcga ggctctcggc gacctccagc
     5641 gactccgggc cgaaagcctc gcggcgcagc gccagggccc gttcgaggag gggctccgcc
     5701 gcgtcgtacc ggccgaggcc gaggttcacc tcgccgatgg tgtccatcac ggcggcctgc
     5761 acgtccggct ggccggacag ctcccgcgcg ctgcgctccg ccccctggtc gaggatctgc
     5821 cgcgccgtga gccgctcccc ctcggtctgg taggggtcgg cctgcttgaa ggtgtcgacc
     5881 aggaacgaca gcgtgctgcg cgccttgtcc cgctcccggg cgagctgccg gccctgggcg
     5941 accaggctgg ccaggaaggc ggccacgatc aggaccacca acgccgccga ggcgatcccg
     6001 gcccggtggc ggcggatgaa cttaccggtc cggtaccgta ccgtcggagg ccgcgcggtg
     6061 accgggcggc cacggagatg ccgctccagg tcctgggcga gctgggcggc cgagccgtac
     6121 cgctgccgcg ggtccttgcg caacgccatc agggcgatgc cgtcgaggtc cccctccagc
     6181 cgccgggcca gggcccgggg gcgcgtccgg cgggcgcgcg cggtctcgtc cacgtcggag
     6241 gggccgggcc ggaccagggc ggcgctgggg ggctccggct cctgctcgca gacggcgcgc
     6301 tcgatgtctt ggggctggtc ccccggggcc cggcagggcc ggcggccggc gagcagctcg
     6361 tacaggatga cgccgagaga gtagacgtcg ctcgccgtgg tgatcgcctc gccccggacc
     6421 tgctcggggc tggcgtagct gggggtcagc atccgcaacc cgtcccaggt ctcgtcgaga
     6481 gcgtccttct cttgcgcgag ccgtttggcg atgccgaagt ccagcagctt cggctccccg
     6541 tccgcggtga ccaggatgtt gcccggcttg aggtcgcggt ggaccagcag gctctgatgg
     6601 gcgtactgga cggcggcgca gatcttctgg aagaggacga gccgctggtc gaccgtcagc
     6661 cggtggtggt cgcagtacag gtcgacgggc attccctcga cccgctccat gaccaggtag
     6721 gggcggccgt cctcggtgct gccgccgtcg tacagccggg cgatgttggg gtgctcgagg
     6781 tgggccagga tctgccgctc ggcgaggaag cgatgatagg cctcggtgct cgcgaggctg
     6841 gcgcggagga ccttgacggc gacgtcccgc tgatagtgct cgtcctcgcg gcgggcgagg
     6901 tagacggtcc ccatgccgcc tctgccgatc cgccgcagca gccggtaggg gccgagcctc
     6961 cccccctcct cccggtcgtc gagcgccagg cgcagcagct caccgggtgg gctctcgagg
     7021 aaatcggtgc cggcctgatc ggcggcgagc agccgctcga cgccgcgccg gagggcggca
     7081 tcgccggcgc aggccgcgtc gaggaaggcg ggccgctccg ctggagcccg ctccagggcc
     7141 tcggcgaaga cgcggtcgac ctcttgccag cgttcgggat cgatcacgga cctacctcga
     7201 acgacccccg cgtcatgagg ccccggattg tagcgcgtcg tacagccagg ccttggcgcg
     7261 gcgccagtcg cggctgaccg tctccgccga gatgccgagc tgctcggcga cctcgtcgag
     7321 gctcaggccg gcgaagaact tgagctccac gaccgccgcc ttgcgcggat ccaccttctc
     7381 gagcgccgag agggcttcgt ccagggccag caggtcggtg cccttctcca gcgccagctc
     7441 ggccacctcg gcgagggaga ccttctcgat ccggccgccc cgtttggccc ggttgcggta
     7501 gcgggcatag tccacgagga cgcggcggat caggtgcgcc gcgaagccga agaagtgggc
     7561 gcggctcggc cactccagcc ctgcctggcc ggagaggcgg agataggcct cgtggacgat
     7621 cgcggtcgcc tgcagcgtgt gggcgtcgcg ctcctggcgg agctgccgca cggcgatgtg
     7681 gcgcagctct tcatagacga gagggaggac ccgctccgcc gcctcctggt ctcccgagct
     7741 ccagcccttg aggagcagag tgatggtcgc ttcggttcct tcgggcaacg gcatatccaa
     7801 catcctcgat tgaagtgcag tcggatcggg aaacatttcg gctcgccccc gtctacgaga
     7861 aggcgctcgc cgcgccggcc gcgcagagga gcgtggccac accgaagagc agcagcgtca
     7921 gggcggtcgg tctcatggat gtcctctatc tatatatacg agtgcggcca gcacggtgtt
     7981 caggtgacgc tccggctctc gggcgccgaa cggaggagag cttccggtcc gccgcttgtt
     8041 cattatagat ccagccacag catttcctct ctcattagca gagattgacg cgaaccgtct
     8101 agtcggtcta cgattcggcc atagcagccg ttaagcgttc ccgaagggcc ggaacggacc
     8161 aggagtggca gggggatctc tggaagaact tagcttccgg cgtcatgaag gcgccgggat
     8221 tcgagccgga aggctcaagg aaggagaagc atcatgtcga aggaagtcac gtatgcggga
     8281 aagcgtgggg cctggcgcca gcgcctgaac acggtggccg cgaacgccgg tgacctcgag
     8341 cacctccagg ggctggcgaa ccggttggag gccacgctgc aggcggtgga gggggtggcc
     8401 cggcagcagg cgatcttcat cgcgggcaag caggagagct cgcagcagct cgcggcgctg
     8461 atgggcgacg gcgagcgtct ggacaccctg ctccgcaagg ggttgcagca gcactacggc
     8521 atcggctcgg agaagctggc cgagttcggc gtgcagccct accgcggccg gacccgcaag
     8581 gcggtgctcc cggtcgagga cacgagcccg ccgtccgccg atcccgccga gtagccgacc
     8641 gctgatttcg tgtcctattg agggggcgtg ccctgcacgc cccctctttt tttgtccctc
     8701 tcctccgtcc ccggcccgca gtcatttccc gcggctcaac ccgaaaaaag gcggcgcgac
     8761 ttcaaatgcc acgggccgac ttcattcgcg gcggcgcggc tcgagaagag gcggcgcgac
     8821 ttcgttgggg atggcccggc tcaaacgcgg cgagccggct tcgtttgcgg cggcgcgact
     8881 tcaaacgcgg cggcaggact tcgtttgaag tggggcgact cgagacgcgg cgaggcgatt
     8941 ccatctgtgg cgggccgact tgaaagacgg cgagccacct tggtttccgg cgaggcgact
     9001 tcgtttggaa tgagacggct tgaaacgcgg cgaggcgact tcgtttcaag cgagctggcg
     9061 gatctggtca gaccaccggc cgcgagcagg acaggcagga ctgcgcgcca cacctcgcgc
     9121 ggagagcgac acgctccgga gatcgacatg cttctcgagt tggacgttcc gcagcgtagc
     9181 acatcggccc gattcttgct gtcgagagac aatgaacgcc gtctcgctcc ttatttgagg
     9241 ttgacaatgt tcatagccaa gaaaagcatc gtggtcctcg cggtcgtcgc cgtgggcctc
     9301 tcgggctgtg cctcctcgcg gcttcccgtc tccgccacga cgggccccga tcccgagctg
     9361 cctcctccca acaagtcgct gattccggtg gtgaacgtcg tcaaggcgat cggctggccg
     9421 gaaggggtca cgccggcggc cgccgagggg acggcggtgg ccgcgttcgc ccgggatctg
     9481 gatcacccgc ggtggctcta cgtgctgccg aacggcgacg tgctcgtggc cgagaccaac
     9541 gcgccgccgc ggccggagga cagcaaggga atcaaggggt ttttcttcaa gctcttccag
     9601 aagaaggccg gcggtgccgt gcccagcgcc aaccgcatca cgttgctgag ggacaccgac
     9661 ggggatggca ccgcggagac gcgcacggct ttcctctccg ggctcaactc acctttcggc
     9721 atggagctgg tgggtgacgt gctctatgtg gcaaacagcg atgctgtggt gaagttcccc
     9781 tatacgcagg gggcgacgca gctcacggca cccgccgtca aagtcgtcga tctccccgcg
     9841 gggccgcgca accatcactg gaccaagaac gtgatcgctt cccgcgacgg ctcgaagctc
     9901 tacgtcgcca tcgggtccaa cagcaacgtg gccgagcacg gcatggcgga ggaggagggg
     9961 cgcgcggcaa tctgggaggt cgacgccgcg accggcagcc atcgcgtgtt cgcctccggg
    10021 ttgcgcaatc cggtgggcat ggcctgggag cccgagagcg gcgcgttgtg gacggcggtc
    10081 aacgagcgcg acgagctcgg caacgatctc gtgcccgact acatgacgtc cgtgcgcgat
    10141 ggcggcttct acggctggcc ctacagctat tacggccaga acgtcgaccg gcgggtgaag
    10201 ccgccgcggc cggatctcgt cgccaaagcc ctcgtgcccg actatgccct ggggccgcac
    10261 actgcctcgc ttggcctggc ccattcgcaa ggcacgtccc tgccggaccg gttcggccgc
    10321 ggcatgttcg tggggcagca cggctcctgg aaccgcaagc cgcgcagcgg ttataaagtg
    10381 atcttcgtcc ccttcagcgg cagccggcca gccggccagc cgctcgacgt gctgaccggc
    10441 ttcgtcaagg agaacggtga ggcgatgggg cgtcccgtgg gcgtcgcggt cgacaagaag
    10501 ggcgcgctgc tggtggcgga tgacgtgggc aacgtcatct ggcgggtcac ctcggcgcgg
    10561 tagcgtgacg ggctggtgcg tagcgggcca tcttgccgaa gaggacgtcc gggctgatgg
    10621 gttttcccac gaactcgttg aagcccgcgg cgtcgatctc gtgctgatgg gccatgcggg
    10681 tcgaggcgga gagcgcgaag atcggcagct tcgcgaacct ctctcccggc agcgagcgga
    10741 tctcccgcgt cgcggcatag ccgtcgagct ccggcatgcg aagatccatc agcaccaggt
    10801 cgtagtcccg cgcacggacg cgctccacgg cctgccggcc gttggacacg acgtcgaagg
    10861 cgacccccca acccttcagc agcccggtca ggacgaagac attgacctcg ttgtcgtccg
    10921 cgaccaggac ctcgagcccc tcgagcccgg aggtcaccag cgcttgggcg ggcgccgtct
    10981 ccgcctccgc caccgtcttg aagcgcagct cgaaggagaa cgtggtcccc ttccctggct
    11041 cgctcgcgac gaggatgcgg ctgccgtgca tctccaccag tttccggctg atcgacaggc
    11101 cgagcccggt gccgccgtat gtcaggccga tgtcgtagtt ggcctgggtg aagtcatcga
    11161 agatgcgcgc cagccggtcc ggagcgatgc cgatcccggt gtcgctcacc cggaaatcga
    11221 tcgataccgc ttccggctcg atcgtacgca cccccagcgc caggatcacc gatccttccg
    11281 aggtgaactt gatcgcgttg ccggccaggt tgctcagcac ctggccgagc ttcatgggat
    11341 ctccggctac ggtccgcggc acccgctcgt ccacgtggac caggagcgcc agccccttcg
    11401 cctgcgcccg gggctcgaag ctctcggcga cctcccggac gagctgccgg agatcgacgg
    11461 accgctcttc cagggacatc ttgccggcct cgatcttgcc gaagtcgagg atctgattga
    11521 ccagatgcag caggtttccc gacgaggagc ccaggatccg cacgagcttc tgttgttgcg
    11581 gcgagagatc ggtttgtcca agcaggtgtg ccacgccggt gatcgcgttg agcggcgtac
    11641 ggatctcgtg actgatcatc gccagaaaat ccgacttcgt ctgcgatgcc tgctcggccc
    11701 gctgcctctc gcgcagcagc tcgcgttcgt attccttgcg gtcggtggca ttgaagaccg
    11761 tggtgcggat cagcaggggc ttttcgtcct cgtccttccg gagcaccgaa ttgatcagca
    11821 ccggcaggcg gtggccgtcc gcgcacacca ggtccagggc gagctcgcgc accgtcccct
    11881 gcatctgcag cagcggcgcg tagtgtgtct cgtggaagat ccggccgccg gcggtgagga
    11941 gctcgtagaa gcgcttgcgg ccggcgagat cttcccgccg gtatcccgtc caggccagaa
    12001 atgtctgatt gaccttgagg atcatcccgc cgggaagcgc cgagagatag ccgcaggggg
    12061 cgttctcgta gaggtcctcg gcgctctcct cgagcaggga gggctgggga ggggagggtt
    12121 ttccgtcctg gtccgacacg gcgagtgaaa acgtctacaa aaagcgctgg atggctgcga
    12181 tcgtctcctc gggagcactc agattggggc agtgcccggt cgcattcatc agaaccagct
    12241 cgctgccggg aatgttccgg tggacgtatt ccccgacgca tcgcggcgcg atcgcgtcct
    12301 cggagcactg caggaccagc gaccggacct tcaccttcgg gaagtcggcc cggttgtcgg
    12361 aaaggaaggt gacgcgggcg aagtggcgcg cgatcgccgg atcggtgcgg cagaagctgt
    12421 tggtcagctc cgtgccgagc tccggccggt ccgggttccc catgatgacc ggggccatgg
    12481 cgctggacca gcccaggtag ttgctgtcga gggactcgag gagcccttcg atgtcctcgc
    12541 ggctgaaccc gccgacgtag tctccgtcgt cgatgtagca gggggacggc cccacgagga
    12601 cgagggcgtc gaaccgttcc ggctcctcgt tcgccgcgag caccccgatg gccgcgctga
    12661 ccgagtggcc gacgaagacc acccgggtca ggtcgagctc gcggcagatt tcgaggacgt
    12721 cgctcgcgta gccctggagc gaggagtact tggcctggtt gtaggcggac aggtcggacc
    12781 ggccggcgcc gacgtggtcg aagaggacga tccggtactc gtcctcgaag gcgggcgtga
    12841 cgaaccgcca catgttctgg tcgcagccga agccgtgggc gaagaccatg ggctggcccg
    12901 tcgcgcggcc ggagagctgg acgttgttcc tctggagcac gttcatgggc ccatgatacc
    12961 gtgccgggcg tctccccccg ggctcttcct tctacatgag gaaaggacgt tccccctgga
    13021 gagagtttct gtgacgtcgg ggtccggcgg ctgataggct gcgccttcga tggagagact
    13081 gagggacgcc ctggctgccg cgatctcctc ccgggcggag gagatgagcg gcttcc
//
LOCUS       DASPJI010000439         8168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_660346, whole genome shotgun sequence.
ACCESSION   DASPJI010000439 DASPJI010000000
VERSION     DASPJI010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8168)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660346"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..6413
                     /locus_tag="VEL74_11905"
     CDS             15..6413
                     /locus_tag="VEL74_11905"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63278.1"
                     /translation="MAVTGEGGSLTYTELDERAGRLAARLRRLGVGPEVRVALWLDRT
                     VELVVAILGVLKAGGAYIPIDPSYPEDRLRFLLADAGTPVLVSVEGLRGWLDVEGITT
                     VLLDRLDEAGAAPSAAPAADLPESTAYVIYTSGSTGRPKGVPVSHANVARLLAATAEW
                     FRFGESDVWTLFHSYAFDFSVWELWGALLHGGRLVIVPYWVSRSPEEFHDLLVRERVT
                     VLNQTPSAFHQLAAVDEGRTAAGRQQELVLRWVIFGGEALEPRRLADWFARHGDCRPT
                     LVNMYGITETTVHVTFRPLGQTDLERGSVIGVPIPDLYIQMLDDEREPVPLGVAGEIH
                     VGGAGLARGYLGRPALTAERFVPDPFSPWPGTRLYASGDLARRRTDGELEYLGRADQQ
                     VKIRGFRIEPGEIEAALASLAGVREAVVVVREDTPGDRRLVAYVAGDIAFLEPGALRR
                     SLQERLPEHMVPAAFVALPTLPLTPNGKLDRQALPAPGWQGPGESYQSPRTPAEEILA
                     GIWAELLALERVGAGDHFFDLGGHSLLATQVMSRVQSAFGVEMQLRDLFEAPVLAALA
                     TRVEAALRSHAGSVAPPLVPVPRQGPLPLSFAQQRLWFIDQLEPGSSLYNMPVALRLE
                     GPLSRAVLERSLGEVVRRHETLRTVFAILDGSPVQEILPAASFELPVIDLSGLPEDGR
                     EETALALTADEAGRPFDLVRGPLLRGLLLRLSAEDHIAALTLHHIVSDGWSMGILVRE
                     ATALYEAFFEGRPSPLAELPMQYADFAVWQRSWLRGATLESEISYWRRQLAGLPPLLE
                     LPTDRPRPAVQSYRGASRPLRLSAGLTRQAQALALRQGATLFMVLLAAFQAVLARYSG
                     QRDFTVGTLIAGRNRTEIEGLIGFFLNTLALRGDLTGALSFRQLVVQARETALAAHLH
                     QDVPFEKLVEELTPERSLAHTPLFQVMLVLQNTPSEELKIRDLHLRSMSEDKATEHFD
                     LSLVLVEHQGELQGGLKYATDLFDASTVERLVVHYERLLTAALNAPDAGIADLTLLSP
                     AERHQLLTEWNDTLVLTPREILLPDLFAAQVARTPDALAATFEEESLTYAQLATRAER
                     VARHLRKLGCGPESRVGVALERNLDLLISFLGVLQAGAAYVPLDPDYPRERLRHLLED
                     SCPTVLLTQESLRDRLPVPDRVPVLLLAALPETDGTIDLATQRDLGDDLLAYVIYTSG
                     STGRPKGAMVVHRGMVNHLWAKVVDLGLEPGCRVAQTASQCFDISVWQLLSPLVVGGS
                     VHIAEETTVHDPELLLRFAARERITVLEVVPSLLSNMFDPLVAADSTIDLSVLEWLLV
                     TGEACPPDLADRWLALAPYTRLLNAYGPTECSDDVTHYERAQPGSGPSLSLPIGRTVA
                     NTRIHVLDPTGQPAALGVSGELCVAGRGVGRGYLGLPARTAQVFVPDAWSGEPGARLY
                     RTGDLARWLADGTLEFLGRLDHQTKVRGFRIELGEIEAVLVAQAGVREAAVVAREDTP
                     GDQRLVAYVVGDAAAEALRQSLRERLPEYMVPGALVSLAALPLTPNGKVDRKALPAPE
                     QQSAGESWVAPRTPIEEVLAGIWAELLGLERVGAADDFFDLGGHSLLATRVKSRLRSA
                     FGVEMPLRVFFEAPTLAGLAALIEVARGTASRRLAPPLTPIAPALREGPLPVSFAQQR
                     LWFIDQLEPGSPLYNMPVALRAEGPLDRSVLARTLTEIVRRHETLRTVFASREGEPVQ
                     VIKPASSFPLPLVDLSGLLESARETALLTLTGEEAGRPFDLARGPLLRGLLLRLAERE
                     HIVALTMHHIVSDAWSLGILVHEVVALYAAFAAGRPSPLPELSVQYADFAVWQHGWLD
                     QEMLEELSEHWRQRLAGVPAQLDLPGARPRPAVLSPRGAARQHLFPPPLLEQLRALGR
                     RESATLFMTLLAPLQALLHARTGATDLVVGTDVAGRDRHETEGLIGFFINQLPLGADV
                     TGDPTLRELLGRAREMALEAYAYQDLPFDQLVDALRVERSLQRAPVFQVKLVLQNAPR
                     EGLDLPGLTFEVVPSSTATAQLDLHWSFVESGDDLWLTLTYSTDLYDEPLIESLLEQY
                     EIWLYAFTERPEARLGEVVAEFAQAERSRRAERGLELRSKGLGQLRSLRRQADSPMEV
                     "
     gene            6417..>8168
                     /locus_tag="VEL74_11910"
     CDS             6417..>8168
                     /locus_tag="VEL74_11910"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG63279.1"
                     /translation="MTESLEVGFRPSPQQLRAWRLQKERPAPCSVLCALRIEGNLDPH
                     ALRRALAATVARHEILRTAFRLPAGLSMPLQVILEPGSVSLPEVDLRSVPEPDRPAGA
                     AALLRSLGDPPFELERGEGWRALLARLGPREHELLLALSAACADPGACEPLAADLLAA
                     YEREVTGGESDPEPPLQYADVAEWQHRLLEGEDAAEGIEAWREQWRVHDIDARLTLPL
                     PLEESEAAPGPFTSRETAVPLDRETAGALARLAGVWDCPLPVLLLAAWHAVIQRGTGR
                     DESLVGVLYAGREAEELRGVVGPLARYLPVAARFSPESPLREAVAQVRQTMTEAEPWA
                     EYFSWDHVIGNGPAAGGFPVCFEAAPGEWELAMNGLRVASVRAGTSALLDRFALALSC
                     QGGEEGLRLTLRYDSRRFRGQAARRLAGRLAALLGSVAARPEAPLADHPALAEEELGL
                     WRELSVAPPAAGAAARGRDGKTLSAVFEEQVDRDPERLAVVAGEERLTFRELDARANR
                     LAWHLRATGAGPEVPVALCLERSAEALVALLAVWKAGAFYVPLDPTQPAGRLAYILTD
                     TGAPLLVTDSRLAAGLPA"
BASE COUNT         1118 a         2916 c         2797 g         1337 t
ORIGIN      
        1 gcacaccgac ggccgtggca gtcaccggcg aggggggctc gctgacctac accgagctcg
       61 acgagcgcgc cggccggctg gcggcccgcc tgcgccgcct cggcgtcggc ccggaggtgc
      121 gagtggccct gtggctcgac cgtacggtcg agctggtcgt ggcgatcctc ggtgtgctca
      181 aggccggagg cgcctacatc cccatcgacc cgtcgtatcc ggaggaccgc ctgcgcttcc
      241 tgctcgccga tgccggcaca ccggtcctgg tcagcgtcga ggggctgcgc ggctggctgg
      301 acgtcgaggg gatcaccacc gtcctgctcg accggctcga cgaggccgga gccgcgccgt
      361 cggccgcgcc cgccgccgac ctgccggagt ccaccgccta tgtcatctac acctcgggct
      421 ccaccggcag gcccaaggga gtaccggtca gccatgccaa cgtcgcgcgc ctgctcgccg
      481 ccacggccga gtggttcagg ttcggcgaaa gcgacgtctg gacgctgttc cactcctacg
      541 ctttcgactt ctcggtctgg gagctttggg gggcgctgct ccacggtggg cgcctggtga
      601 tcgtccccta ctgggtgagc cgctcgcccg aagaattcca cgacctgctg gtacgggagc
      661 gggtgaccgt gctcaaccag acaccctcgg cattccacca gctcgccgcc gtggacgagg
      721 ggcggacggc ggcggggcgg cagcaggagt tggtgttgcg ctgggtgatc ttcggcggcg
      781 aggccctgga gccgcgccgg ctggccgact ggtttgcccg ccacggcgac tgccgcccga
      841 cgctggtcaa catgtacggt atcaccgaga ccaccgtgca cgtgacgttc cgtcccctcg
      901 gccagaccga cctggagcgg gggagcgtca tcggcgtgcc gattccggac ctctacatcc
      961 agatgctcga cgacgagcgc gagccggtgc ccctgggcgt agcgggcgag atccacgtcg
     1021 gcggcgccgg tctggcccgc ggctacctgg gccggccggc cctcaccgcc gaacgcttcg
     1081 tgcccgaccc gttctccccc tggcccggca cacgcctcta cgcctcgggc gacctcgccc
     1141 ggcggcggac ggacggagag ctcgagtacc tggggcgggc cgaccagcag gtcaagatcc
     1201 gcggcttccg catcgagccg ggcgagatcg aggcggcgct cgcttcactg gccggagtcc
     1261 gtgaggcggt cgtggtggtg cgcgaggaca cgcccggaga ccggcggctg gtcgcctatg
     1321 tggcgggcga catcgccttc ctggagcccg gagcgttgcg ccgctctctg caggaacggc
     1381 tgccggaaca tatggtgccg gcagccttcg tcgcgctgcc cacgctgccc ctcaccccca
     1441 acggcaagct ggaccgccag gcgctgccgg cgcccggatg gcaggggccc ggggagagct
     1501 atcagtcgcc gcgcacgccg gccgaagaga tcctcgccgg gatctgggcc gagctactcg
     1561 ctctcgaacg ggtcggagcg ggcgaccact tctttgacct gggcggtcac tcgctgctgg
     1621 cgacgcaggt gatgtcgcgc gtccagagcg ccttcggcgt cgagatgcag ctccgcgatc
     1681 tgttcgaagc tcccgtcctt gcggccctcg ccacccgggt agaggcagcg ctgcggagcc
     1741 acgccggcag cgtggctccg cccctggtcc cggtgccgcg acaggggccc ctgcccctgt
     1801 ccttcgcgca gcagcgtctc tggttcatcg atcagctcga accgggcagc tcgctctaca
     1861 acatgcccgt ggccctgcgc ctcgaggggc cgctgagccg cgcggtgctg gaaaggagcc
     1921 tcggcgaagt cgtgcgccgt cacgagacgt tgcgtacagt cttcgcaatc ctggacggct
     1981 caccggtaca ggagattctt ccggcggcct ccttcgagct gcccgtgatc gatctctcgg
     2041 ggctgccgga agacggccgt gaggagacgg cgctcgccct gaccgcggac gaggcgggcc
     2101 gtccgttcga tctcgtccgc gggccgctgc tccgcggcct gttgctgcgc ctgagcgcag
     2161 aggaccacat cgccgcgctg actctgcacc acatcgtcag tgacggttgg tcgatgggca
     2221 tcctggtccg cgaggccacg gctctgtatg aggccttttt cgagggccgg ccctcccctc
     2281 tcgccgagct cccgatgcag tacgcggact tcgcggtctg gcaacgttcc tggctgcgcg
     2341 gcgccaccct ggagagcgag atctcctact ggcgccggca actcgcgggt ctgcctcctc
     2401 tgctggagct cccgacggat cgcccgcggc ccgcggtgca gagctatcgc ggcgcttcac
     2461 gccccctccg gctgtccgcc gggctcactc ggcaggcaca ggccctggcg ctacgccagg
     2521 gggcaacgct cttcatggtg ttgctggctg cattccaggc cgttctggca cgctacagcg
     2581 gtcagagaga ctttacggtc ggcaccctga tcgccggacg aaaccggaca gagatcgagg
     2641 gtttgatcgg cttcttcctc aatacgctgg ccctgcgcgg cgatctgacc ggcgctctct
     2701 cgttccggca gctggtcgtc caggcccgcg agactgcgct ggccgcccat cttcaccagg
     2761 acgtgccgtt cgaaaagttg gtggaggagc tcacacctga gaggagtctc gcccacaccc
     2821 ctctgttcca ggtgatgctg gttctccaga acactccctc cgaggagctg aagatccggg
     2881 atctgcacct gcggtcgatg agtgaagaca aggcgacgga gcatttcgat ctgtcgctcg
     2941 tcctcgtgga gcaccagggc gagctacaag gcgggctgaa gtacgccacc gatctcttcg
     3001 acgcgtccac cgtcgaacgt ctggtcgtcc actacgagag gctgctcacc gccgccctga
     3061 acgcgccgga cgcagggatc gcagatctga cgctgctttc ccccgccgag cgccatcagc
     3121 tcctcaccga atggaacgat acgctggtgc tgacgccgcg ggagatcctc ctccccgacc
     3181 tgttcgcggc tcaggtcgcg cgtacaccgg acgcactggc cgcgaccttc gaggaggaga
     3241 gtctcaccta tgcccagctc gctacccggg cagagcgagt ggcgcgccac ctgcggaagc
     3301 tgggatgcgg cccggagagc cgcgtcggtg tcgcactgga gcgcaacctc gacctgctca
     3361 tctcgttcct cggcgtgctc caggctggag ccgcctacgt gccgctggac ccggattatc
     3421 cgcgcgagcg cctacgccat ctgctggaag actcctgtcc aacggtcctc ctgacccaag
     3481 agagcctgcg ggaccggctg cccgttccgg accgcgtgcc ggtattgctc ctcgcagcac
     3541 tgccggagac ggacggcacg atcgatctgg cgacacagcg ggacctcggc gacgatctcc
     3601 tggcctacgt catttacacc tcgggctcga ccggtcgccc caagggagcc atggtggtgc
     3661 accgcggcat ggtcaaccat ctttgggcca aggtcgtgga tctgggcctc gaacccggct
     3721 gtcgggtggc acagaccgct tcccaatgtt tcgacatctc ggtctggcag ctcctctccc
     3781 ctctcgtggt cggcgggagc gtgcacatcg ccgaggagac caccgtccat gaccccgagt
     3841 tgttgctgcg tttcgccgcc cgggaacgga tcaccgtgct cgaagtggtc ccctccttgc
     3901 tgagtaacat gttcgacccg cttgtcgcgg ccgacagcac gatcgatctt tcggttctgg
     3961 aatggttgct cgtgaccggc gaggcgtgcc caccggatct agccgaccgc tggttagcgc
     4021 tggcgccgta cactcgcctg ctcaacgcct atggcccgac cgagtgctcg gacgatgtga
     4081 cgcactacga gagggcccag ccgggctccg ggccgtcgct ctccctacca atcggcagga
     4141 cggtcgccaa tacgcggatc catgtgctcg atcctaccgg ccagccggcg gcactaggcg
     4201 tctccggcga gctctgcgta gcggggcgcg gcgtggggcg tggctatctc ggcctgccag
     4261 cccgcacggc gcaggtcttc gttccggatg cctggagtgg cgagccgggc gcacgcctgt
     4321 atcgcaccgg cgatctggca cgctggctgg ccgacggaac tctggagttc ctgggccggc
     4381 tggaccatca gaccaaggtg cgaggtttcc gcatcgagtt gggagagatc gaggccgtcc
     4441 ttgtcgcgca ggccggagtc cgcgaggcgg ccgtcgtggc gcgcgaggac acgcccggcg
     4501 atcagcgcct ggtcgcctat gtcgtcggtg atgccgcagc ggaggcgctg cgccagtcac
     4561 tgcgcgagcg cctgcccgag tacatggtgc ccggcgccct cgtctccctc gcggcgctcc
     4621 ccctgactcc caacggcaag gtcgatcgca aggccctacc cgctccggag cagcagagcg
     4681 ctggcgagag ctgggtggcg ccgcgcacgc cgatcgaaga ggtcctcgcc ggcatctggg
     4741 ccgagctcct cggcctggaa cgggtcggag ccgcggacga tttcttcgat ctgggcggcc
     4801 actcgctcct ggcgacacgg gtgaagtcac gcctgcgcag tgccttcggt gtcgagatgc
     4861 cgctgcgcgt gttcttcgag gcgccgacgc tggcgggcct cgcggcattg atcgaggtgg
     4921 cgcgcggcac cgcctcgcgc cgcctcgcgc cgcctctgac gcccatcgcc ccagcgctgc
     4981 gggaggggcc tttgcccgtc tctttcgcgc agcagcgact ctggttcatc gatcagctgg
     5041 agccgggcag tccgctatac aacatgccgg tggcgctacg tgcagaaggg ccgctcgacc
     5101 gcagcgtgct ggcgcgcacc ctcaccgaga tcgtgcgccg tcacgagacg ctgcgcacgg
     5161 tcttcgcctc gcgcgaggga gaaccggtac aagtcataaa gccggcgtcg tccttccctc
     5221 tgcctctggt ggatctgtcg gggctgctgg agagcgcgcg cgagacggca ctcctcaccc
     5281 tgaccggtga agaagccggc cggccgttcg atctcgcgcg cggcccccta ctgcgcggcc
     5341 tgctgctgcg gctggcggag agggagcaca tcgttgcact cactatgcac cacatcgtga
     5401 gcgacgcctg gtcgctgggc atcctggtcc acgaggtcgt ggcgctctat gccgcgtttg
     5461 cagcgggtag gccttcgccc ttgccggagc tgtccgtgca gtacgccgac ttcgctgtct
     5521 ggcagcacgg ctggttggat caagagatgc tggaggagct gagcgagcat tggcgtcagc
     5581 gcttggccgg ggtgccggcg caactcgacc tgccgggagc ccgcccgcga ccggcagtgc
     5641 tgtcgccgcg cggggccgcg cgccaacatc tcttcccccc gcccctgctg gagcaactcc
     5701 gcgctcttgg ccgccgcgag tccgcgaccc tgttcatgac tctgctagca cctctccagg
     5761 ccctcctcca cgcccgcacc ggcgccaccg acctggtcgt ggggacggac gtggccggac
     5821 gcgaccgcca cgaaaccgag ggtttgatcg gctttttcat caaccagctc cctttaggcg
     5881 ccgacgtgac cggcgatccc actctgcgag agctgctggg gcgggctcgc gagatggcac
     5941 tggaagcgta cgcctaccaa gacttgcctt ttgaccaact cgtcgatgca ctgcgggtgg
     6001 agcggagcct ccagcgcgct ccagtcttcc aggtgaagct ggtgctccaa aatgcccctc
     6061 gggaaggcct ggatttgccg ggcttgacct tcgaggtcgt accctcgagc actgcgacgg
     6121 ctcagctcga cctccactgg agctttgtgg agagtggcga tgacctttgg ctgactctca
     6181 cctacagcac ggacctttac gacgagccgc tgatcgagag cctgctcgag cagtatgaga
     6241 tctggctcta cgccttcacg gagcgaccgg aggcacggct cggcgaggtg gttgccgagt
     6301 tcgcccaggc ggaacgctcc cggcgagcgg agcgcggcct ggagctcagg tcgaaaggtc
     6361 tcggccagtt gcgaagcctg cgccggcagg cggattcccc catggaggtc tgatcaatga
     6421 cggaatcact cgaggtgggc ttccggccct cgccgcagca gctgcgtgcc tggcggctgc
     6481 agaaagagag gcctgccccg tgctctgttc tttgcgctct gcgcatcgag gggaatctcg
     6541 atccgcacgc cttgcgccgt gccctggccg ccaccgtggc gcgacacgag atcctgcgta
     6601 ccgccttccg gctgccggcc ggtctcagca tgccgctcca ggtcatcctg gagcccggct
     6661 ccgtgtccct ccccgaagtg gatctgcgga gcgttccgga gccggaccgg ccggccggcg
     6721 ccgccgctct cctccgttcc ctgggagacc cgccgttcga actcgagcgc ggcgagggtt
     6781 ggcgcgccct cctggcgcga ctcggccccc gcgagcacga gctgctgctc gcgctgtccg
     6841 ccgcctgcgc cgatccgggc gcctgcgagc ccctggcggc cgacctcctc gccgcctatg
     6901 aacgcgaggt gaccggcgga gagagcgacc cggagccgcc cctccagtac gccgacgtgg
     6961 cagagtggca gcacaggctg ctggaggggg aagacgcggc ggaggggatt gaggcctggc
     7021 gggagcagtg gagggtccat gacatcgacg cccggctcac cctgccgctc cccctggagg
     7081 agagcgaggc ggcgcccggc cccttcacgt cgcgagagac ggccgtccct ctggatcgcg
     7141 aaaccgctgg cgcgctggcg cgtctggccg gggtatggga ctgcccgctg ccggtcctgc
     7201 tcctcgccgc ctggcacgcc gtgatccagc gcggcacagg ccgtgacgag agcctggtag
     7261 gggtgctcta tgcgggacgc gaggcggagg agctgcgagg cgtggtcggc cccctggccc
     7321 gctatctgcc cgtcgcagcc cgcttcagcc cggagtcccc gctgcgggag gccgtggccc
     7381 aggtccggca gaccatgacg gaagccgagc cgtgggcgga atacttcagt tgggaccacg
     7441 tgatcgggaa cggcccagcg gccggcggtt tccccgtctg cttcgaagcc gcgccgggag
     7501 agtgggagct ggccatgaac ggcctgcggg tcgcctcggt ccgtgccggc accagcgccc
     7561 tcctcgaccg cttcgctctc gccctgtcgt gccagggcgg ggaggagggc ctccggctca
     7621 ccctgcgcta cgactcgcgg cgctttcgcg gccaggcggc gcgccgcctg gccggccgcc
     7681 tggccgcgct gctgggctcg gtggccgccc ggcccgaagc cccgctagcc gaccatcccg
     7741 cgctcgcaga ggaagagctg ggcctctggc gggagctgtc ggtggcgccg cccgctgccg
     7801 gggccgccgc tcgcggtcgg gatggcaaga ccctctccgc ggtcttcgag gagcaggtgg
     7861 accgcgaccc ggagcgtctc gccgtggtgg ccggagagga gcgtctgacc ttccgcgagc
     7921 tcgatgcgcg ggcgaaccgc ctggcctggc acctgcgcgc cacgggagcc gggccggagg
     7981 tgcccgtggc cctgtgcctg gagcgctcgg ccgaggcgct ggtcgccctc ctagccgtct
     8041 ggaaggcggg cgccttctac gtccccctcg atcccaccca gccggcgggg cgcctggcct
     8101 acatcctcac cgatacggga gcgcctctcc tggtcaccga ctcgcgcctg gccgccggcc
     8161 tgcccgcc
//
LOCUS       DASPJI010000440        14884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_85479, whole genome shotgun sequence.
ACCESSION   DASPJI010000440 DASPJI010000000
VERSION     DASPJI010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14884)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14884
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85479"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="VEL74_11915"
     CDS             <1..1403
                     /locus_tag="VEL74_11915"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HYG63280.1"
                     /translation="GVPHTSLRWHYRSTHESLITFSNVSFYDGGLFTFPSTETDSHAR
                     GVQFEYVEDGVYEGKGVNLTEARRVADAVMRHARETPDLSLGVGTFSLRQQLAIQDEL
                     EARRRQDPSAEGFFAPKEEGGFFVKNLENIQGDERDVIFLSVTYGRGPDGKIRHHFGP
                     INGENGWRRLNVLTTRARRHMRVFSSMRGDEINSAQTTSRGAKLLHEFLLYAEHGRLE
                     VPMEAAAGSDDERLLEKDVHNELTRHGIRLQPRVGMAGYRIDFGVLDDAAPGRFLCGI
                     ECDGPAYRDAETVRDRDRLRHEVLEARGWTLLRVWSTDWFKDRPGQVDRLLRQIEEAR
                     QRAALIPAPSPMIEMEDVRAGLSLARDGASPSPTSENSSPQARLPFPQYPPASRYAGL
                     PAESIPPEAYREMIVGVLRQAGSLERSELIKEVRALLGYKRTGSKLEKAIGSVIDDLL
                     ARGVLGEGSSGLRLRE"
     gene            1415..2920
                     /locus_tag="VEL74_11920"
     CDS             1415..2920
                     /locus_tag="VEL74_11920"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYG63281.1"
                     /translation="MLALVNAVDPFISTLALVGVVVIVSALLSGLIERSGVPQVGVLL
                     ALGAILGPVGLGLIDVTLDSPVLRAVSVLSLALVLFTDALSINPAEVRQHSKLTLLIL
                     GPGTILAAALISFAAWWLLGLSVPNAAILGAALASTDPVLLRGLLRRRDIPTVARLGL
                     KLESGLNDVVLLPIVLVAMVFLGSRHIDDGRDWSSMGIQLFLLGPGAGVVVGLLGVAA
                     LDLVRRRIGVRRDYESLYSLGIAFAAYAGAEALHGSGFLAAFAAGLTISIFDVDLCDC
                     FMEYGETTSELALLFTFVLLGSSQIWSGLTVLSGPALLFALIAMLARPAALLAALGPA
                     RLPRRSLILITWFGPRGLSSLLLVLLPVFAGLPGSDQLFAYCSLVVLLSVLIHGGSLM
                     LVGRWSLTGKTAGPAETAPSAPPSPSPLRVLPSEPDLPSPTEERISLNEVRRLWDQGE
                     PVIVLDVRRERALAESDSTVQGAVRLDPDRAVADAERLHLPKEAWLIAFCA"
     gene            3091..3393
                     /gene="yhbY"
                     /locus_tag="VEL74_11925"
     CDS             3091..3393
                     /gene="yhbY"
                     /locus_tag="VEL74_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HYG63282.1"
                     /translation="MEKLKGSQRKWLRGMAHALKPVVQIGKQGLTEGVISQVDTALAD
                     HELIKVQAVASKEEKQEVGRQLEEATGAEVVGLIGHILILFRQNADPEKQLYELPE"
     gene            complement(3762..4118)
                     /locus_tag="VEL74_11930"
     CDS             complement(3762..4118)
                     /locus_tag="VEL74_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63283.1"
                     /translation="MKEIVQRSPLTAFVLIAFGITWPLAAGMRYSLLLPLLGLFGPLI
                     GAFVVLWALEGREGLRGIWRRFALRRHHAKWLVAAVVLPLLLLVPVWPVDRSGSGALP
                     DADRAGGHRSGLPDRG"
     gene            4537..6132
                     /locus_tag="VEL74_11935"
     CDS             4537..6132
                     /locus_tag="VEL74_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYG63284.1"
                     /translation="MTRHPGEWGEELAASTLSGPSRAPGAGAARVPGLTLVAHPDVRR
                     VGERVALPEISSGRAVELSRLVPLFSPPDGGAPEPLADLHLSRNPLLLGPGTEPGSVL
                     LRRGGAPTSLEVDGRPVGSEHLCSAADVERGAVLLLAERIVLLLHRMPPAPPREAERF
                     GLLGDGAAMARLRADIRRVADLEVAVLLRGETGTGKELVARAIHRSSPRRSRPYLAVN
                     MGAIPPPLAAAELFGAARGAFTGADQRRTGYFSQAHNGTLFLDEIGETPAEVQALLLR
                     ALETGEVQPVGAEAPRRVDVRVLAATDADLEEAIAAGRFRAPLLHRLNGYEIRLPALC
                     ERREDFGQLFFAFLRQELETLGEEERLADPGPRGAAWLPAPLVARLAAADWPGNVRQL
                     RNAARQIAISNRGAAQAVLPPAVLGPLERQRAGASGERPAAPAPRPTYRRPADVGEEE
                     LLAALAANRWKLVATAAQLGLSRTALYTLVDRCAGIRKAADLDRAEIAEAMARHGKDL
                     DAMVEELRVSRHGLQLRMTELGL"
     gene            6140..8692
                     /locus_tag="VEL74_11940"
     CDS             6140..8692
                     /locus_tag="VEL74_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYG63285.1"
                     /translation="MESSRVGPYLLTGRLGAGGMGVVYDAWDERLERRVAIKRVQPDP
                     AGGPPDPRRRERLRREARAAARLSHPAIVQVFDVVETDEADWIVLELAEGPTLAELLR
                     QGPLDFARLLPLARELAEGLAEAHGRGILHRDLKAENVVVTRSGHAKILDFGLAKRFW
                     PAASGSGDGLSREGEIVGTPRAMSPEQANGEALDPRSDLFSLGTLLYETVTGVSPFAA
                     ATPVETLHRVCSHQPPPAAALNPAAPLVLSELIGQLLEKDRERRPADASQVATRLAAL
                     QESCSGSGARSSRPELEQEPTVADVTVPAPRRLLSKSRAGWKRWGAGGLILLVVGATV
                     LAWVLRHREPLYVAVAEPQVPAGAADAETAALAAAGLRAALLRDLSGLNRIAVLTPEP
                     GSSPAPRSLARELAADEVLVPRLDCAGRDCRITLSRLLAADGRLLRSETFSAPVDDLL
                     SIAAAVGPALRRAYAGFSWRPAIAGREIGRSEWERYVRLNREYWEHRGGSPPQRLLAE
                     AGALRRESPRFVEVILLEANLRSLRFFASRDPVDAKRARELLDKARRLAPGDPRPLRL
                     LAIVTVSSEQLGDAEAAIAELEKRLPGDAGAGVLRAHLDERAGRPDEALAGMRAAVRR
                     RPGQSNLLGLANMESRLGRMDEARRTLATLLQRFPHDRNARSFLAQLELGSGSAETAA
                     RLYAELVRTEPTFTELTNLGVAQLLLGRYDTAAASFRQALGLVPDSPAAVLNLADAEW
                     LRGRKTEAAALYRNVIDLAARDPAPTFWQTQTIRAQALAHLGRRDEAVAAVQQALRAA
                     PDNPQVAYEASLVYALVGDAASARVNAGRARAAGYDRRWFSFPWFDRVGLER"
     gene            complement(8699..9109)
                     /locus_tag="VEL74_11945"
     CDS             complement(8699..9109)
                     /locus_tag="VEL74_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63286.1"
                     /translation="MNTTQREVNVKFRDNRVSFSGSGISQDGRILIPTESGLIVLRLK
                     TSRVRFPSFPIQWTIQQSAAPPATGTEFAPIAQPSEATVIRKSDRESDIRIVSPEGTR
                     EFSFFVLVETPDGKFFGTDPTIVTMRPPDPNDAR"
     gene            9368..11164
                     /locus_tag="VEL74_11950"
     CDS             9368..11164
                     /locus_tag="VEL74_11950"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HYG63287.1"
                     /translation="MIIQTPFVRRLLPLALACGLAAMAPATQAQPAPDLFTPLPTEAP
                     RGLNATQRQYLGHIEQRPTTISVTLGRVDVAALERDKMRITVSPTKALVLTKDEVTAR
                     DEAGFTWVGSFPGASGQTILVVSDGNITGTIRDETTFYQLEPVGGGMHALVAVDPSRF
                     PPDDSPEFNREQKRRRTGPDKTPVFVPRTNQSLVEIDVLVAYTTKAKATVADIDAMVD
                     LAIAETNQAYKNSGIAIELKLVDSFELVGYSETGKSPADVLADFKGRQEVNTRRAFSG
                     ADVAVLLENLNTDNCGLADVILAGPSTAFAVVKIYCAAANLSFAHEVGHLQGARHNPE
                     NDTNDWPFSYGHGLQHPASNATQRFRTVMAYPCTTPCDPRISYFSTPNRSYNGVPVGT
                     AARNDNARVLNETAKQVADFMNRPFHGYRFCALEHGRCGFYGTKNVAYGANGKFYYKT
                     AAGGLDCNNGTFGDPIFVNKACYVGPEFYDFCAKEGERCNFTGTMNVAYGTDGKFFYR
                     TATSGIDCNNGTFGDPIKENKACYVGPVGTTFCATQDDQCSFVGSKTVVFGTHGYWVS
                     TRATDGVRCDTSTFGEVVFPDGVKASCYIVVE"
     gene            11212..12348
                     /locus_tag="VEL74_11955"
     CDS             11212..12348
                     /locus_tag="VEL74_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HYG63288.1"
                     /translation="MISKNAVFFLTLFLAAALPAAAADPKPMTPQISPESPATVDAAA
                     AERLAKLALACVHKEYPNKIAHVLNGDGDVKPPRELTPAFYGCYDWHSAVHGHWLLAR
                     LARRFPEAPFAAPARAALARSLTPANIAAEVKYIEGTGRATFERPYGLAWLLQLDAEL
                     TEWDDPQARQWAATLHPLTQAAVGRLRTWLPKLSHPIRTGEHNQTAFGLGLILDWARS
                     KAGDPEAAALFETRARDLFLGDRLCPIDYEPSGEDFLSPCLGQADLVRRLLTPAQFAV
                     WLEAFLPQIPRDGGTGWLTPGVVTDPTDGKLAHLDGLNLSRAWMLEGIASGLPQGDPR
                     LPALRAAAAAHRESGLRAVTGEHYEGGHWLGSFATYLVTGRGLK"
     gene            complement(12424..>14884)
                     /locus_tag="VEL74_11960"
     CDS             complement(12424..>14884)
                     /locus_tag="VEL74_11960"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYG63289.1"
                     /translation="NIDGAEANSDFFGEERGGTEAPFVFSQAAIREFQVIRSTYSAEY
                     ARGVGATINAVTKSGTNELEGELFFFRRDSDWGDERSPTVNGLPVTETAEARDSDQYG
                     LALGGPIVRDRLFFFVNADFQDISEPLLAPDVRTTSRFTSLPADVQQAFLTRVEGLIG
                     NSLDNEVRFDSRQDQETYLVKLDGNLATSHHASLRYNSSDYNNFPSEGSGDILSNNGD
                     EFNKVESTVLQADSIFGSSVANQLIVQYGIEERPIFAFTDNLPETIVSEVNNFTFGRS
                     EFLPNRTDETKWQIKDQLSYAVGRNQFKVGAEYLQTEIDNLFPREAGGQWMFNTVADF
                     LANRPSRLDQGYGPTLGLNSFDYDSWGAFLQDTLSVGERLTLDFGVRYDFQSIPEPVG
                     NVYPTRPEFLQSFNDDDDNFAPRFGFAYDVRGDGRSVLRGGVGQYYNYLPSILYAAPL
                     AEIAGLYNRISVNCATATCPTYPAILTPEQFAVFARTASTVTTVSPDLEATESLRSSL
                     GFEQQIGASFSVGLEAVYAQIDNAQRLINSNIAPAGFSYGNLPVYSQTSPSRPYPELQ
                     AVRQHRSDGEGEYTSLTVSARKRAIGALPLTLLAHYTWSEAIDQDSNERSTSSSFSLD
                     PFNPSLSEGRADYDVTHRVVMSGTYELPWGVMLSGIFLWRSGTPYTAGIDTGFIGLNG
                     IDSQSVDTPVFVDGSGNVIDLTLANGMSPQQLAAFLAGQGARMEDRNERDQPDFTNFD
                     LRLSKSFDFNIIELELIGEVFNVFNTENEFITTTNQTMFTGTLSNNAWRFTRNPNFGR
                     ANAYNNLGVPRQYQAAVRVRF"
BASE COUNT         2248 a         5182 c         5144 g         2310 t
ORIGIN      
        1 cgggcgtccc ccacaccagc ctgcgctggc actaccggag cacccacgag tcattgatca
       61 cgttttcgaa cgtcagcttc tacgacggcg gcctcttcac cttccccagc accgagaccg
      121 acagccacgc gcggggcgtc cagttcgaat atgtggaaga tggcgtctat gagggcaagg
      181 gggtgaacct gacggaggcc cggcgagtgg ccgacgcggt catgcgccac gcccgggaga
      241 cgccggacct ctccctgggc gtcgggacgt tcagcctgcg ccagcaactc gccatccagg
      301 acgagctcga ggcgcggcgc cgccaagacc cgagcgccga ggggttcttc gcgccgaagg
      361 aggagggggg cttcttcgtc aagaacctgg agaacatcca gggcgatgag cgtgacgtca
      421 tcttcctctc cgtcacctat ggtcgaggtc cggacggcaa gatccgtcac catttcggac
      481 cgatcaacgg cgagaacggc tggcggcgcc tgaacgtgct gaccacccgg gcgcgccggc
      541 acatgcgggt cttctcctcc atgcgcgggg acgagatcaa ctccgctcag accacctcgc
      601 gcggcgccaa gctgctgcac gagttccttc tctatgccga gcacggccgc ctggaggtcc
      661 ccatggaggc ggcggccggc agcgacgacg agcggctgct ggagaaggac gtccacaacg
      721 agctgacccg gcacggcatc cgtctccagc cgcgggtggg gatggcgggc tatcggatcg
      781 acttcggcgt cctggacgac gccgcgcccg gccgcttcct ctgcggcatc gagtgcgacg
      841 gcccggccta cagggatgcc gagacggtgc gcgaccgcga ccggctgcgc cacgaagtgc
      901 tggaggcccg gggctggacg ttgctgcggg tctggtcgac cgactggttc aaggaccgtc
      961 cgggacaggt ggatcgcctg ctgcggcaga tcgaggaggc ccggcagcgg gcggccctca
     1021 tccccgcccc ctctcccatg atcgaaatgg aagatgtacg ggcggggctt tccctcgccc
     1081 gggacggggc gagcccctcc cctacatcag agaattcgtc cccccaggcc cggctcccct
     1141 tcccccagta ccctccagca agccgctacg ccggcctgcc cgccgagagc atcccgcccg
     1201 aagcctatcg ggagatgatc gtcggggtcc tgcggcaagc cgggagcctg gagaggagcg
     1261 agctgatcaa ggaggtccgc gccctcctgg gctacaagcg cacgggatcg aagctcgaaa
     1321 aagcgatcgg ttccgtgatc gacgacctgc tggcccgcgg ggtcctgggc gaaggcagct
     1381 cggggctccg gctgcgggag tgaatcggag gaccatgctc gccctcgtca acgccgtcga
     1441 tccattcatc tccaccctgg ccctggtcgg cgtggtggtc atcgtctcgg ccctgctctc
     1501 cggcctgatc gagcggagcg gggtccccca ggtcggggtg ctgctggctc tcggggcgat
     1561 cctcgggccg gtgggcctgg ggctgatcga tgtcaccctg gactcgccgg tgctgcgcgc
     1621 ggtctccgtg ctcagcctgg cgctggtgct gttcaccgac gcgctgtcga tcaacccggc
     1681 cgaggtccgg cagcacagca agctgaccct tttgatcctc gggccgggga cgatcctggc
     1741 ggcggcgctg atcagcttcg cggcctggtg gctcctcggc ctctcggtcc cgaacgccgc
     1801 catcctgggc gccgcgctcg cctcgaccga tccggtgctg ctgcgcggcc tgctgcgccg
     1861 cagggacatc ccgacggtgg cccggctggg gcttaagctc gagagcggcc tgaacgacgt
     1921 ggtgctcctg ccgatcgtgc tggtcgccat ggtctttctg gggagccgcc acatcgacga
     1981 cgggcgggac tggtcgagca tggggatcca gctcttcctg ctcggacccg gggccggcgt
     2041 ggtggtgggg ctcctgggag tggcggcgct cgacctggta cggcggcgca tcggcgtgcg
     2101 acgcgactac gagtccctct attcgctggg gatcgccttc gccgcctatg ccggcgccga
     2161 ggcactccac ggcagcggct tcctggccgc cttcgccgcc ggcctgacga tctcgatctt
     2221 cgacgtcgac ctctgcgact gctttatgga gtacggcgag accacgtcgg agctggcgct
     2281 cctcttcacc ttcgtgctgc tcggcagctc gcagatctgg agcgggttga cggtgctgag
     2341 cggtccggcg ttgctcttcg cgttgatcgc catgctggcc cggccggccg ccctgctggc
     2401 cgcgctggga ccggcgcggc tgccccgtcg cagcctgatc ctgatcacct ggttcggacc
     2461 gcgcggcctg agctcgctgc tcctggtgct gctcccggtc ttcgccggcc tcccgggcag
     2521 cgatcagctc ttcgcctatt gcagcctggt ggtcctgctc tccgtcctga tccacggcgg
     2581 ctcgctgatg ctggtcggcc gctggagtct caccggcaag accgccgggc cggcggagac
     2641 cgcgccgtca gcgccaccct ccccttcccc cctgcgcgtc ctcccttcgg agccagacct
     2701 cccctccccg accgaagaaa ggatcagcct caacgaagtc cgccgcctct gggaccaggg
     2761 agagccggtg atcgtgctgg acgtgcggcg cgagcgggcg ctggcggaga gcgattcgac
     2821 ggtccagggg gccgtgcggc tcgatccgga ccgcgccgtc gccgacgcgg agcgcttgca
     2881 tctgccaaag gaggcttggc tgatcgcctt ctgtgcctga aaggccgaag ccacgagcgc
     2941 ccgtgtggcg ctcgagctcc ggcggaccgg ctggccccgc gccttcgccc tcaccggcgg
     3001 ctgggaagcc tggcaggccg ccggcctgcc gatggagccc ttgaagaagg agtgaggggg
     3061 ccggttcagc cccgtgctag catccggcgc atggagaagc tcaagggttc ccaacggaag
     3121 tggctgcgtg gcatggccca cgcgttgaag ccggtggtcc agatcggcaa gcagggtctc
     3181 acggaggggg tgatcagcca agtggacacg gcgctcgccg accacgagct gatcaaagtc
     3241 caagccgtcg cctccaagga agagaagcag gaagtcgggc ggcagctcga ggaggccacc
     3301 ggcgccgagg tggtcggcct catcggccac atcctcatcc tgttccgtca gaacgccgac
     3361 cccgagaagc agttgtacga gctgccggag tgagcatcgc cgcttcgcgg ccgggccgtg
     3421 ttcccaccat ggccgcaacg gcaccgtata ccgccgccct gcaccagtac tcccgcacgg
     3481 gatcgatccc ggagagagaa aagaggctca acgcggcgtg gaacaccacg gccagcggaa
     3541 ggcttccgtc cgtcctgaga gacagccacg tgaacagaac cgaatacgaa acgatcagca
     3601 gcacgaaggc ggtgaacgga aggccgtgat gagggtagcc tggtagaaag aagttcggca
     3661 gatgccagag agcccacacc aggccgagca ccaggctgct cgcgagagcc ggccatcgtc
     3721 cgaggagata gggcagagcg aagccccgcc atcccacctc ctcacccacg atcaggaagg
     3781 ccagaacgat gcccaccggc gcgatcggcg tcaggaagag cgccggagcc ggaccggtca
     3841 accggccata cgggaaccag caacagcagt gggagcacca cagcggccac caaccacttc
     3901 gcgtggtgac ggcggagggc gaatctccgc cagatccccc tcaggccttc acgaccttcg
     3961 agtgcccaga ggacgacgaa ggcgccgatc aatgggccga agaggccaag cagcggaagc
     4021 agcagcgaat agcgcatgcc ggccgcaagg ggccaggtga tgccgaaggc gatcagcacg
     4081 aacgctgtca acggagaccg ctgaacgatc tctttcatca gccgcggccg aagcgtgccg
     4141 cgatgccgcc gaagagcagg attttcatcg gggctcagag tagcacgggg tgcgggagcg
     4201 gaccctcccc ggcaccgctc ccgcgtgcgc cttaccggcc cggcggcgcc cgtctcgggc
     4261 caagccgtgc gcgtgtcggg cgaagccagg cctctcgcag gccgcgccac gccctcgaca
     4321 ggcgaagcca tgcctgcctc cggccacgcc atgcctgcgt caggcacggc cgagccctcg
     4381 acaggcatgg tggtgcccgc ctcgggcacc gccgtgcccg cgacaggccg cgccatgcca
     4441 gccgcgggcg acgccacgcc cgtgtcaggc gtggccgtgc ccacctcacc agcggtcctt
     4501 gccccctcgg ctctccctgt ttagactgct cgcatcatga cgcgtcatcc cggggagtgg
     4561 ggggaggagc tggcggccag cactctttca ggaccctcca gagctcctgg cgcgggcgct
     4621 gcacgcgtcc ccgggctcac gctcgtggcg catccggacg tccgccgggt cggcgagcgc
     4681 gtggcgctgc ccgagatctc ctcggggcgc gccgtggagc tgtcgcggct ggtccctctg
     4741 ttctctccac cggacggcgg cgctcccgag cctctcgccg atctccattt gagccggaac
     4801 cctctgctcc tcggcccggg gaccgagccc ggatcggtgc tgctgcgccg cggcggcgcg
     4861 ccgaccagcc tggaggtgga cggccggccg gtggggagcg agcacctgtg ctcggcggcc
     4921 gatgtcgaac ggggagccgt gctactgctc gccgagcgga tcgtcctgct gctccaccgg
     4981 atgccaccgg cgccgccccg ggaggccgag cggttcgggt tgctcggaga cggcgccgcg
     5041 atggcccggt tgcgcgcgga catccggcgc gtcgcggacc tggaggtcgc cgtcctgctg
     5101 cggggagaga ccggcacagg caaggagctg gtcgcccgcg cgatccatcg gagcagcccg
     5161 cggcgctccc gtccctatct agcggtcaac atgggcgcga tccctccccc gctggctgcc
     5221 gcggagctgt tcggcgcggc gcgcggcgcc ttcaccggcg ccgaccagcg gcgcaccggc
     5281 tatttcagcc aggcgcataa cggcacgctg ttcctcgacg aaatcggcga gaccccggcg
     5341 gaggtgcagg ccctgctgct gcgcgccctg gagaccggcg aggtccagcc ggtgggggcg
     5401 gaggcgccgc ggcgcgtgga cgtgcgggtc ctcgctgcca cggacgcgga cctggaggag
     5461 gccatcgccg ccggccgctt ccgcgcgccg ctcctccacc ggctgaacgg ctacgagatc
     5521 cgtctcccgg cgctgtgcga gcggcgagag gatttcggcc agctcttctt cgcctttttg
     5581 cgccaggagc tcgaaaccct gggagaggag gaacggctgg ccgaccccgg gccgcgcggc
     5641 gccgcctggc tgccggctcc cctggtcgcc cgcctggccg ccgcggactg gccgggcaat
     5701 gtgcgccagc tccgcaacgc ggcccgccag atcgcgatta gcaaccgggg cgctgcgcag
     5761 gccgtgctgc cgccggccgt gctgggaccc ctcgagcgcc agcgcgccgg cgcctccggc
     5821 gagcggccgg ccgcgccggc tccccgcccc acctaccggc gccccgcgga cgtgggagag
     5881 gaagagctgc tggccgcgct cgccgccaat cgctggaagc tggtcgccac cgcggcgcag
     5941 ctcgggctct cgcgcaccgc gctctacacc ttggtcgatc gctgcgccgg catccgcaag
     6001 gcggcggatc tggaccgggc cgaaatcgcc gaggcgatgg cgcggcacgg gaaggatctg
     6061 gacgccatgg tcgaagagct gcgggtctcc cgtcatgggc tgcagctccg catgaccgag
     6121 ctggggctgt aggagacgct tggaaagctc ccgcgtcggc ccctatctgc tcaccggccg
     6181 gctcggcgcc ggcggcatgg gtgtggtcta cgacgcctgg gacgagcggc tggagcgtcg
     6241 cgtcgccatc aagcgggtgc agcccgatcc ggccggcggt ccgcccgatc cgcgccgccg
     6301 cgagcgcctg cgccgggagg cccgcgccgc cgcccggctg tcccatccgg ccatcgtcca
     6361 agtcttcgac gtcgtggaga cggacgaggc ggactggatc gtcctcgagc tggcggaggg
     6421 gccgaccctg gccgagctgc tgcgccaggg gccgctggat ttcgcgcgcc tgctcccgct
     6481 cgcccgcgag ttggcggagg gcctcgccga ggcgcacggc cgggggatcc tgcaccggga
     6541 cctcaaggcc gaaaacgtgg tggtcacccg ttcgggtcac gcgaagatcc tggacttcgg
     6601 cctcgcgaag cgcttctggc ccgcggcctc cgggtccggc gacggcctct cgcgcgaagg
     6661 cgaaatcgta ggcacgcccc gggccatgtc tcccgagcag gccaacggcg aggcgctcga
     6721 tccgcgttcg gacctcttct ctctcggcac cctcctctat gagacggtca ccggcgtctc
     6781 cccgttcgcg gccgccacgc cggtcgagac gctgcaccgg gtctgctccc accagccgcc
     6841 gccggcggcg gcgctcaacc cggccgcccc cctggtcctc tccgagctga tcggccagct
     6901 cctggagaag gaccgggagc gccggcccgc ggacgcgtcc caggtggcca cccggctcgc
     6961 cgcgctccag gagagctgca gcggcagcgg cgcccgctcc tcgcggcccg agctcgaaca
     7021 agagcccacg gtcgcggacg tcaccgtgcc cgccccgcgc cgactcctgt cgaagtcccg
     7081 ggcgggatgg aagcgatggg gcgccggcgg gctcatcctc ttagtagtgg gcgccaccgt
     7141 gctcgcctgg gtcctgcgcc atcgcgagcc cctctacgtc gccgtggcgg agccgcaggt
     7201 ccctgccggg gccgccgatg ccgagacggc ggcgctcgcc gcggcaggtc tccgggccgc
     7261 cctgttgcgt gacctctccg ggctcaaccg gatcgcggtc ctgacgccgg agcccggctc
     7321 ctcgccggcg ccgcgaagcc tcgcccggga gctggccgcg gacgaggtgc tggtgccgcg
     7381 cctcgactgt gccggacggg actgccggat caccctgagc cgccttctgg ccgcggatgg
     7441 acgcctgctg cggagcgaaa cgttctcggc gccggtcgac gacctcctgt cgatcgccgc
     7501 cgcggtcggc ccggcgctcc ggcgggccta tgcgggcttc tcctggcgtc cggcgatcgc
     7561 gggccgagag atcgggcgca gcgaatggga gcggtacgtg cggctcaacc gggagtattg
     7621 ggagcaccgc ggcggctcgc cgccgcagcg cctcctggcc gaggcggggg cgttgcggcg
     7681 ggagtcgccg cgtttcgtcg aggtgatcct cctggaggcg aacctgcgga gcctccgctt
     7741 cttcgcctcg cgggacccgg tggacgcgaa gagggcgcgc gagctcctgg acaaggcgcg
     7801 gcgcctcgct cccggcgatc cccggccctt gcggctcctc gcgatcgtca ccgtgtcctc
     7861 cgagcagctc ggcgacgccg aagccgccat cgccgagctc gaaaagcggc tcccgggaga
     7921 tgcgggggcg ggcgtcctgc gggcgcacct cgacgaacgc gcgggccggc cggacgaagc
     7981 tctggcagga atgcgcgccg cggtccggcg ccgcccggga caatccaatc tcctgggcct
     8041 ggcgaatatg gagtcgcgcc tgggacggat ggacgaggcg cggcggaccc tggccaccct
     8101 cctccagcgt ttcccgcatg atcgcaacgc ccgctcgttc ctggcgcagc tcgagctggg
     8161 ctccggcagc gccgagaccg cggcccgtct ctacgccgag ctggtgcgga ccgagcccac
     8221 cttcaccgag ctcacgaacc tgggagttgc gcagctcctg ctggggcgat acgacaccgc
     8281 ggcggcgagc ttccggcagg ccctcggtct cgttccggac agccccgcgg ccgtcctcaa
     8341 tctcgccgat gccgaatggc tgcgcggccg gaagaccgaa gcggcggcgc tctatcgcaa
     8401 cgtgatcgat ctggcggccc gcgatcccgc gcccaccttc tggcagaccc agaccatccg
     8461 cgcccaggcg ctggcccacc tgggcaggcg cgacgaggcg gtggccgccg tccagcaggc
     8521 gctccgcgcc gcgccggaca atccccaagt cgcgtacgag gcgtccctgg tctacgccct
     8581 cgtcggcgat gccgcgtcgg cgcgcgtcaa tgccgggcgg gcgcgcgccg ccggctatga
     8641 ccggcgctgg ttttccttcc cctggttcga ccgcgtcggc ctcgagcgct gacccgctct
     8701 accttgcatc gttaggatca ggagggcgca tggtgacgat cgttggatcg gtgccgaaga
     8761 actttccatc aggggtctcg acgaggacga aaaatgagaa ttcccgggtc ccctccgggc
     8821 tcacgatccg aatatcgctc tccctatcac tctttcggat gaccgtggcc tcggaaggct
     8881 gggcaatcgg cgcaaactcc gttcctgtcg ccgggggagc cgcggattgc tgaatggtcc
     8941 actggatcgg gaaggaggga aagcggacac gggatgtctt gagccgaagg acgatcagtc
     9001 cgctttcagt agggatgaga atccggccgt cctgcgagat ccccgatcct gaaaaggaga
     9061 ccctgttgtc tctaaacttg acattgacct cacgctgcgt ggtgttcatg ccgttcctcc
     9121 ttgcttggat ggccggaagc ctagcatgcc tcggagccga ttgtcggacc gtccggcgga
     9181 cggtccgaca gtgatggaca gtcggcgccg ctcctgcgct cgacctatta attcctttgt
     9241 tttcagccac ttgaccctcc atgtaccacg ggtacggcgc ttgcccttcc agcggggcat
     9301 ccgcccggaa gcgcgtggct cccgcggcgg cggaagagaa agattttgat ttgaaaagga
     9361 gggtttcatg atcatccaga cgccgttcgt ccgccgtctt ctccctctcg ccctggcctg
     9421 cggcctcgcc gcgatggctc cggccactca ggcccagccc gctcccgacc ttttcacgcc
     9481 tctgcccacg gaagcgcctc ggggcctgaa cgcgacgcag agacagtatc tcggccacat
     9541 cgagcagcgc cccactacga tttcagtcac cctcgggcgc gtagacgtgg ccgcgctcga
     9601 gagggacaag atgcgcatca ccgtgtcgcc gacgaaggcg ctcgtcttga cgaaggacga
     9661 ggtgacagcg cgcgacgagg cgggcttcac ctgggtcggc tcgttccccg gcgcctccgg
     9721 ccagacgatc ctcgtcgtca gcgacggcaa catcaccgga accatccggg atgagacgac
     9781 cttctatcag ctcgagccgg tcggcggcgg catgcacgcg ttagtcgccg tcgacccctc
     9841 gcgcttccca ccggacgact ctcccgagtt caaccgcgag cagaagcgcc gtcgcaccgg
     9901 accggacaag accccggttt tcgttccgcg gacgaatcaa agcctcgtcg agatcgacgt
     9961 gctggtcgcc tatacgacca aggcaaaggc gacggtggca gacatcgacg cgatggtgga
    10021 cctcgcgatc gcggagacaa accaagccta caagaacagc ggcatcgcca tcgagctgaa
    10081 gctcgtggac agcttcgagt tggtgggcta ttcggaaacc gggaagtcgc ccgcggacgt
    10141 cctcgccgac ttcaagggca ggcaggaggt caacacgagg cgggcgttca gcggcgcaga
    10201 cgtcgccgtc cttctggaga acctcaacac cgacaactgc gggctggcgg acgtgatcct
    10261 ggccggacca tcgactgcct tcgccgtcgt caaaatctac tgcgcggccg caaacctgag
    10321 cttcgctcat gaggtcggcc atctacaggg cgcgcggcac aacccggaga acgacacgaa
    10381 cgactggccc tttagctacg gtcacgggct tcagcacccg gcgagcaatg cgacgcagcg
    10441 cttccgcacg gtcatggcct acccctgcac cacaccctgc gatccgcgga tttcgtactt
    10501 ctcgaccccg aatcggagct acaacggcgt ccccgtgggg acggcggccc ggaacgacaa
    10561 cgcccgcgtg ctcaacgaga ccgcgaagca ggtggccgat ttcatgaacc gcccatttca
    10621 cggttatcgc ttctgcgcgc tcgagcacgg gcgctgcggc ttctacggca ccaagaacgt
    10681 cgcatacggt gcgaacggca aattctacta caagacggcg gccggcggcc tcgactgcaa
    10741 caacggcacc tttggtgatc cgatcttcgt gaacaaagcc tgctatgtcg gacctgagtt
    10801 ttacgacttt tgcgcaaaag agggcgagcg ctgtaatttc accggcacca tgaacgtcgc
    10861 ctacggtacg gacggcaagt tcttctatag gacggccacc agcggtatcg actgcaacaa
    10921 tggcaccttt ggcgatccca tcaaggagaa caaggcctgc tacgtcgggc ccgtgggaac
    10981 cacgttctgt gccacccagg acgaccagtg cagcttcgtc gggtccaaga ccgtcgtctt
    11041 cggcacccac ggatactggg tctcgacccg cgcgaccgac ggcgtgagat gcgacaccag
    11101 caccttcggc gaagtcgtct tcccggacgg tgtgaaagca tcctgctata tcgtcgtcga
    11161 gtgagctcga agtcgtgagg ttcgggcggc gcccggtggt accatccgcg cgtgatctcc
    11221 aagaacgccg ttttcttcct cactctgttt ctggccgcgg ccctgccggc cgcggccgcc
    11281 gatcccaagc ccatgactcc ccagatctct cccgaatctc cggcgacggt ggacgccgcg
    11341 gcggccgagc ggctggcgaa gctggccctg gcctgcgtcc acaaggagta ccccaacaag
    11401 atcgcccatg tgctcaacgg cgacggcgac gtcaagccgc cgcgcgagct gactccggcg
    11461 ttctatggct gctacgactg gcactcggcc gtgcacggtc actggctgct ggcccgcctg
    11521 gcgcgccggt tcccggaggc tcccttcgcc gcaccggcgc gggcggcgct ggccaggagc
    11581 ctgacgccgg ccaacatcgc ggccgaggtg aagtacatcg aggggacggg gcgcgccacc
    11641 ttcgagcgcc cctacggcct cgcctggctg ctccagctcg acgccgagct gaccgagtgg
    11701 gacgacccgc aggcgcggca gtgggcggcg accctccatc cgctgaccca ggcggcggtg
    11761 ggacgcctcc gcacctggct ccccaagctc tcccacccca tccgcaccgg cgagcacaac
    11821 cagaccgcct tcggcctggg gctgatcctc gactgggcgc ggagcaaggc gggtgatccc
    11881 gaggccgcgg cgctgttcga gacccgcgcc cgcgacctgt tcctcggcga ccgcctctgc
    11941 cccatcgact acgagccctc gggcgaggac ttcctctccc cctgcctggg ccaggccgat
    12001 ctggtccggc ggctcctcac tcccgcccaa ttcgccgtct ggctggaggc cttcctgccc
    12061 cagatcccgc gggacggcgg caccggctgg ctcacccccg gcgtggtcac cgatcccacg
    12121 gacggcaagc tcgcccatct ggacgggctc aacctgagcc gcgcctggat gctcgaaggg
    12181 atcgcctcgg gcctgcccca gggcgacccg cgcctccccg ccctgcgcgc cgccgcggcc
    12241 gcccatcgcg agtccggcct gcgcgccgtc accggcgagc actacgaggg cggccactgg
    12301 ctcggcagct tcgccaccta cctggtcacc ggacgcgggt tgaagtaggt cgcgccgcgc
    12361 accccccaat aaaaagggcc accctcgcgg gtggccctct tcacttcagc ctggtgggtt
    12421 cggttagaac cgcacccgga ccgccgcctg gtactgccgc ggcacgccga ggttgttgta
    12481 cgcgttcgcc cggccgaagt tcgggttgcg ggtgaagcgc caggcgttgt tcgacagggt
    12541 gccggtgaac atcgtctggt tggtggtggt gatgaactcg ttctcggtgt tgaagacgtt
    12601 gaagacctca ccgatcagct cgagctcgat gatgttgaag tcgaaggact tcgagagccg
    12661 caggtcgaag ttcgtgaagt ccggctggtc gcgctcgttg cggtcctcca tgcgcgcccc
    12721 ctgcccggcc aggaaggccg cgagctgctg cggcgacatg ccgttggcga gcgtcaggtc
    12781 gatgacgtta ccgctgccgt cgacgaacac cggggtgtcc acgctctggg agtcgatgcc
    12841 gttcaggccg atgaagccgg tgtcgatgcc ggccgtgtag ggggtgccgc tgcgccagag
    12901 gaagatgccg gagagcatga cgccccaggg cagctcatag gtgccggaca tgacgacgcg
    12961 gtgggtgacg tcgtagtcgg cgcgcccctc gctcagcgag gggttgaagg gatcgaggct
    13021 gaacgagctc gaggtgctgc gctcgttgga gtcctggtcg atcgcctcgg accaggtgta
    13081 gtgggcgaga agggtcagcg gcagggcgcc gatggcgcgc ttgcgggccg agacggtcag
    13141 cgaggtgtac tcgccctcgc cgtcgctgcg gtgctggcgg accgcctgca gctctgggta
    13201 cggccggctg gggctggtct ggctgtagac cggcaggttg ccgtagctga acccggcagg
    13261 cgcgatgttg gagttgatca gccgctgggc gttgtcgatc tgcgcgtaga ccgcctcgag
    13321 gccgacgctg aacgacgcgc cgatctgctg ctcgaagccg aggctgctgc gcaggctctc
    13381 cgtcgcctcc aggtccggcg agacggtggt gacggtgctg gcggtccgcg cgaagacggc
    13441 gaactgctcc ggcgtcagga tggcgggata ggtcggacag gtcgcggtgg cgcagttgac
    13501 gctgatgcgg ttgtagaggc cggcgatctc ggcgagcggc gccgcgtaga ggatcgacgg
    13561 caggtagttg tagtactggc cgacgccgcc gcgcagcacg gagcggccgt ccccccggac
    13621 gtcataggcg aagccgaagc gcggggcgaa gttgtcgtcg tcgtcgttga aactctgcag
    13681 gaactcgggg cgggtgggat agacgttgcc caccggctcg gggatcgact ggaagtcgta
    13741 gcggacgccg aagtcgaggg tcagcctctc gcccacagac agggtgtcct ggaggaaggc
    13801 gccccagctg tcatagtcga aggagttcag gcccagcgtc gggccgtagc cctggtccag
    13861 gcggctcggc cggttggcca ggaagtccgc gacggtgttg aacatccact gtccgcccgc
    13921 ctcgcggggg aagaggttgt cgatctcggt ctgcaggtac tcggcgccca ccttgaactg
    13981 gttgcggccc acggcgtagc tgagctggtc cttgatctgc cacttggtct cgtcggtgcg
    14041 gttgggcagg aactcgctgc ggccgaaggt gaagttgttc acctcgctga cgatggtctc
    14101 gggcaggttg tcggtgaagg cgaagatggg ccgctcctcg atgccgtact gcacgatgag
    14161 ctggttggcc accgagctgc cgaagatcga gtcggcctgc aggacggtgc tctccacctt
    14221 gttgaactcg tcaccgttgt tcgacaggat gtcgcccgag ccctcgctgg ggaagttgtt
    14281 gtagtcggac gagttgtagc gcagcgaggc gtggtgcgag gtggccaggt tgccgtccag
    14341 cttgaccagg taggtctcct ggtcctgccg gctgtcgaag cggacctcgt tgtcgagcga
    14401 gttgccgatg agcccctcca cccgcgtcag gaacgcctgc tggacgtcgg ccggcaggga
    14461 ggtgaagcgg gaggtggtgc ggacgtccgg cgccagcagc ggctcggaga tgtcctggaa
    14521 gtcggcgttg acgaagaaga agaggcggtc gcgcacgatg gggccgccca gcgccagacc
    14581 gtactggtcg gagtcgcggg cctcggcggt ctcggtgacc ggcaggccgt tgaccgtggg
    14641 cgagcgctcg tcaccccaat cggagtcgcg gcggaagaag aagagctcgc cttccagctc
    14701 gttggtgccg ctcttggtga cggcgttgat ggtggcgccg acgccgcggg cgtactcggc
    14761 gctgtaggta gagcggatga cctggaactc gcggatcgcc gcctggctga acacgaacgg
    14821 cgcctcggtg ccgccgcgct cctcgccgaa gaagtcggag ttggcctcgg cgccgtcgat
    14881 gttg
//
LOCUS       DASPJI010000441         8456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_491114, whole genome shotgun sequence.
ACCESSION   DASPJI010000441 DASPJI010000000
VERSION     DASPJI010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8456)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8456
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491114"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VEL74_11965"
     CDS             complement(<1..342)
                     /locus_tag="VEL74_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HYG63290.1"
                     /translation="MSEDHAGDLPPGDLPDHELRRALHRAADRVADYLGRVGDYPVLP
                     PVSPGDVAGALPAAPPASPETLDRLLDDYERLIEPNVTHWNHPGFLAYFAITGSAPGI
                     LGELLAAGLNVN"
     gene            complement(439..1524)
                     /locus_tag="VEL74_11970"
     CDS             complement(439..1524)
                     /locus_tag="VEL74_11970"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HYG63291.1"
                     /translation="MKAFFSNWTARRSASGAVLILALGVWLPACSAQDKAAAGQNAQG
                     GNRELARVNGKVITEADVQATAKDAFAQLDRQYQQQRRELLEGTLNQAVQQRLVEAEA
                     AAKGVTKEQLLAEVKPAPVTDADVDAFYEQNKAQIPPNMTKEAVTPRIKQYLEGQRKT
                     EAEEGFYKQLEAKYKVDYLLEPDRVEVAATGPSKGPANAPVTIVEFSDFECPFCSRIN
                     PTLEQVRAKYGDKVRLVFRQYPLSFHQNAQKAAEASLCAHEQGKFWEMHDAMFQNQQA
                     LAVDQLKTAAAGLGLDAGKFNTCLDSGQYAAQVKADFEAGSAAGVSGTPAMFINGRFI
                     NGAVPLEQITKVIDDELKRKGVATNAP"
     gene            complement(1688..2572)
                     /locus_tag="VEL74_11975"
     CDS             complement(1688..2572)
                     /locus_tag="VEL74_11975"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63292.1"
                     /translation="MSALQATRETARRGRLYPSVILHGAAAATRLAAAVDLGRTLLCD
                     AAPEERPCGVCRHCRRIGAPGEEEGGGKKEAPFHPDFQVLERDLKTSTSVEATRELLK
                     GAQVSPFEARGQVFVVASAETLSGEAANALLKNLEEPHVSAPRHFFLLAPSRLDLLPT
                     LRSRSLAVFLGPAEPVDPESLEPLARGFAAALEAYAGSGAPIYLLTAAEALLQAGKAG
                     GWEDPRSGRPWAMAASAVLRSLDLAPGAPPGTAGRRGRLALAEALLEGPSMRLRSVSA
                     ERILEGLVAKYLASGTGI"
     gene            complement(2596..3243)
                     /gene="tmk"
                     /locus_tag="VEL74_11980"
     CDS             complement(2596..3243)
                     /gene="tmk"
                     /locus_tag="VEL74_11980"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017254069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HYG63293.1"
                     /translation="MRPRFITFEGLDGSGKSTHLRRAAAWLAERGIPHVVTHEPGGTP
                     LGDALRAVFLDPQWGSVDGTVELLLVFASRRQHLIEVIEPALAEGRVVLCDRFTDSTR
                     AYQGYGRGVPLDLIEQVDRLATGGRRPDFTLLFDLPAEDARARGHSPARRGRGTADRL
                     DAEDLGFYGRVRQGFLDLVAREPGRFRLVDSSGSAARTQERVLAILESLLGEGAA"
     gene            3494..3799
                     /locus_tag="VEL74_11985"
     CDS             3494..3799
                     /locus_tag="VEL74_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HYG63294.1"
                     /translation="MASKKASTAGKKASGGEKQGGGAFMKPMQPDDALAAVIGSDPIP
                     RTEVTKRIWDYIRSNNLQDPNDKRTIRADAKLKPVFGGKESVSMFEMTKLVNGHLKG"
     gene            3924..4985
                     /locus_tag="VEL74_11990"
     CDS             3924..4985
                     /locus_tag="VEL74_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HYG63295.1"
                     /translation="MEATAAIGTPLQWGMFLLLVGVMLALDLGVINRKEHEIGLREAL
                     FWSVVWTVIALIFNGWVYYEFGSQAGLEFLTGYIIERSLSFDNIFVFIVIFNYFAVPA
                     RYQHRVLFWGILGALISRGIFIAMGTALLTRFDWLIYVFGAFLVYTGFKILKEKETEV
                     HPENNPVVKLFQRLMPMTAEYHGKRFFVRQAGRRVATPLMLVLVVVEATDVVFAVDSI
                     PAVFGVTQNPFIVFTSNIFAILGLRALYFLLAGLMHKFRYLGVGLGLVLVFVGAKMLI
                     HHWYVIPTVWSLALVMLILTVAIVVSLLRPASPDEVPHAPAPGEPADFQHSQAARELH
                     LSPAADEPPAHPRGSGGAA"
     gene            5064..6206
                     /locus_tag="VEL74_11995"
     CDS             5064..6206
                     /locus_tag="VEL74_11995"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HYG63296.1"
                     /translation="MVTIFIPREQRPGETRVAATPESVRRMVKQGLEVMVERGAGTAA
                     FYPDSGYEAAGGRLVDDAAAGWSTADAVFKVNAPEPGEGAGQRPGSLLVAFLAPYQNL
                     DLVRRLADGGISSIAMELIPRITRAQSMDALSSQASIAGYKAVLIAAGRLGKYFPLLM
                     TAAGTIPPARVVVMGAGVAGLQAIATARRLGAVVEVSDVRAVVKEQVESLGGKFIELP
                     EMPSGEGSGGYAKEMGEEFLRRQRAIVKERIAASDVVITTALVPGRRAPVLVTADMVE
                     GMRAGSIIVDLAVEQGGNCELSRPNEEVVHQGVLILGPSNLPGTLPHDASNLYARNVL
                     TLFQTLCKKDGGIVIDTADEVIAGSLLTHGGKVHHGPTAALLEVPA"
     gene            6324..7007
                     /locus_tag="VEL74_12000"
     CDS             6324..7007
                     /locus_tag="VEL74_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63297.1"
                     /translation="MTRDDGHGPAFALVENSLPQSEESPLSVRLAAAEAALERERAEA
                     PLLCRLLLSHPPERRPEVAAGDPRFQTWGVGERLLARSLELLERDTAEAEHLAVLALT
                     VARRLGRHSPALVRDLEARAWACAGKARLRSGDLAGAGEALRAAAACLAQGTGDLLVE
                     ALLLEFEADVCQAQGHRGEAAALLHQASSRYDQAGDTPQSARLARRRDQLLDDGGSRH
                     SAVPGLGRR"
     gene            7422..>8456
                     /locus_tag="VEL74_12005"
     CDS             7422..>8456
                     /locus_tag="VEL74_12005"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HYG63298.1"
                     /translation="MRLLPLLLLAAVCLPLAIPARAAAPPAPIGTGGAVSSADPAATV
                     AGLEMLRAGGNAVDAAVATALALAVVYPEAGNLGGGGFAVVRMGEQVSTLDFREAAPA
                     AARRDMYLDPKGEPIPQASFVGPLASGVPGSPAGLHELHRRHGKLPWPQVVAPAIRLA
                     RAGLRVDRHLHEVLDQKKEVLARFPETARVWLRDGEAPPVGTVIPLPTLAATLSAYAG
                     QGPAALASGPLAAALEATSRKHGGILTAADLAAYRPVWRDPVLFEAFGWRFASMPLPS
                     SGGIILGQTLGALERLEWAKLPPLGAERHHLLTEVWRRAFADRYLLGDPGTTRATAAQ
                     LLDPAWIGKRA"
BASE COUNT         1165 a         3041 c         3022 g         1228 t
ORIGIN      
        1 gttgacgttg agccccgcgg ccagaagctc ccccaggatg ccgggggccg agccggtgat
       61 ggcgaagtag gcgaggaacc cggggtggtt ccagtgggtg acgttgggct cgatcagacg
      121 ctcgtagtcg tcgagcagcc ggtccagggt ctccggggac gcgggcggcg ccgccggcag
      181 ggcgccggcc acgtcgccgg gggagaccgg cggcagcacg gggtagtccc ccacccggcc
      241 gagatagtcg gccacccggt cggcggcgcg gtggagcgcc cgccggagct cgtggtccgg
      301 gaggtctccg ggcggaaggt ctccagcatg gtcttcggac atggggggat tgtccgggat
      361 cggccggaaa gagaaaaggg gcggccggca cgtggccgga cgcccctccg cgatccgagc
      421 ggtctgacgc ggtgcgggtt acggggcgtt ggtcgccacg cccttgcgct tgagctcgtc
      481 gtcgatcacc ttggtgatct gctcgagcgg cacggcgccg ttgatgaacc ggccgttgat
      541 gaacatggcc ggggtgccgc tgacgccggc ggcggagccg gcctcgaagt cggccttgac
      601 ctgggcggcg tactggccgg agtcgaggca ggtgttgaac ttcccggcat cgaggccgag
      661 gccggcggcg gccgtcttga gctggtcgac ggcgagcgcc tgctggttct ggaacatggc
      721 atcgtgcatc tcccagaact tgccctgctc atgggcgcag agggaggcct cggccgcctt
      781 ctgggcgttc tgatggaagc tcagggggta ctgacggaag accaggcgca ccttgtcccc
      841 gtacttggcc ctcacctgct ccagggtcgg attgatgcgg gagcagaacg ggcactcgaa
      901 gtcggagaac tcgacgatgg tcaccggcgc gttggcgggt cccttggagg gaccggtcgc
      961 cgccacctcg acccggtcgg gctcgaggag gtagtccacc ttgtacttgg cctcgagctg
     1021 cttgtagaag ccctcttcgg cctcggtctt gcgctgcccc tccaggtact gcttgatccg
     1081 gggggtcacc gcctccttgg tcatgttcgg cggaatctgc gccttgttct gctcgtagaa
     1141 agcatccacg tcggcgtcgg tgaccggggc cggcttgacc tcggcgagga gctgctcctt
     1201 ggtgacgccc ttggccgcgg cctcggcctc gaccagacgc tgctggacgg cctggttgag
     1261 cgtgccctcc aagagctcgc ggcgctgctg ctggtactgc cggtcgagct gggcgaaggc
     1321 gtccttggcg gtcgcctgca cgtcggcctc ggtgatgacc ttgccattga cgcgggcgag
     1381 ctcgcggttg ccgccctggg cgttctggcc ggcggcggcc ttgtcctgag ccgagcaggc
     1441 cggcagccag acgccgagcg cgaggatcag gaccgcgccg gatgccgagc ggcgcgctgt
     1501 ccagttactg aagaatgctt tcatgaggtg actcctttgg ccattagctt tccaatacaa
     1561 cctgcatacg aacgtcgcgg actctccacg tcgaaatgga cgggatattt tagcacaagc
     1621 ccagactagc gcccggaccg aagccgacgg ttgagctctc gccgggggag aacctgaaac
     1681 cgtctcctca aattccggtg ccgctggcca ggtatttcgc gacaagccct tcgagaatgc
     1741 gttcggccga gacgctgcgc aggcgcatcg agggcccttc gaggagcgcc tcggccaggg
     1801 cgagccggcc gcggcgcccc gccgtaccag ggggagcgcc gggggcgagg tcgagcgagc
     1861 gcaggacggc cgaggcggcc atcgcccagg ggcggccgga ccgcgggtcc tcccagccgc
     1921 cggccttgcc ggcctgcaga agcgcctcgg ccgcggtcag gagatagatg ggggcgccgc
     1981 tgccggcgta ggcttcgagg gcggcggcga agccccgcgc cagcggctcc aggctctccg
     2041 ggtcgaccgg ctcggccggc ccgaggaaga cggcgagcga gcggctgcgc agcgtcggca
     2101 gcaggtcgag gcgcgacggc gccagcagga agaaatggcg cggcgccgag acgtggggct
     2161 cctccaggtt cttgagcagg gcgttggccg cctcgccgga gagggtctcg gcgctggcga
     2221 cgacgaagac ctgcccgcgc gcctcgaaag gggacacctg ggcgcccttg agcagctccc
     2281 gggtcgcctc gaccgaggtg gaggtcttga ggtcccgctc cagcacctgg aagtcgggat
     2341 ggaagggcgc ctccttcttc ccacccccct cctcctcccc gggggcgccg atccgccggc
     2401 agtgccggca gacgccgcac ggccgctcct ccggcgccgc gtcgcagagc agggtgcggc
     2461 cgaggtctac ggcggcggcg agccgcgtgg cggcggcggc gccgtgcagg atgaccgaag
     2521 ggtagagccg cccccggcgg gcggtctccc gcgtggcctg cagcgccgac atctgcggta
     2581 tctccggcaa cgcgctcacg ccgccccctc ccccagcagg ctctccagga tggcgaggac
     2641 ccgctcctgg gtcctcgccg ccgaccccga cgagtccacc agccggaacc gccccggctc
     2701 ccgcgccacc aggtcgagga agccctgccg cacccggccg tagaacccca ggtcctcggc
     2761 gtccaggcgg tcggcggtgc cgcgcccccg ccgcgccggc gagtggccgc gggcgcgggc
     2821 gtcctcggcc ggcaggtcga agagcagggt gaagtccggc ctccggccgc cggtcgccag
     2881 ccggtccacc tgctcgatga ggtcgagggg gacgccgcgg ccgtagccct ggtaggccct
     2941 ggtggagtcg gtgaagcggt cgcagagcac gacgcggccc tcggcgaggg cgggctcgat
     3001 gacctcgatc aggtgctggc ggcggctggc gaagaccagc agcagctcca ccgtgccgtc
     3061 gacgctcccc cactgcgggt cgaggaagac ggcgcgcagg gcgtcgccca gcggcgtccc
     3121 gcccggctcg tgggtgacga cgtgcgggat gccgcgctcc gcgagccagg ccgccgcccg
     3181 ccgcaggtgg gtcgacttgc cgctgccgtc gagcccctcg aaggtgatga accggggacg
     3241 catgcggggg caacttagca cgaaccgggg ctggagggcc ctcgatcgac ctccagggag
     3301 gacacagagg aggccaaaat agacatccgg ctattctgga ggaggagcgg agggccaatc
     3361 tcgcctaaaa tggtgggcag ggagcacgat caacggaggg actccccggg gccggaattt
     3421 gcatattgtt ccggagcgag tcgtataccc cggtccgtca actctcaacc atctcatact
     3481 gaggaggagc agcatggcaa gcaagaaggc gagcacggca ggaaagaaag cgtccggcgg
     3541 cgagaagcag gggggcggtg ccttcatgaa gccgatgcag ccggacgatg ccctcgcggc
     3601 ggtgatcggg tccgacccca tcccgcgcac ggaagtcacc aagaggatct gggactacat
     3661 ccgctccaac aacctgcagg accccaacga caagcggacc atccgtgccg acgcgaagct
     3721 caagccggtc ttcggcggca aggagtcggt cagcatgttc gagatgacca agctggtcaa
     3781 cggccacctc aagggctgag gctttccacc ccgcccaggc cggtacggag gcgcccccgg
     3841 cggggcgcct ccgtgttttc ccccccttcg agtacactcc cccggttcgg gagcacagcc
     3901 cggcgacgga gacccccagc cccatggaag ccacagccgc gatcggtacc cccttgcagt
     3961 ggggcatgtt cctcctcctg gtcggggtca tgctggccct cgacctcggg gtcatcaacc
     4021 gcaaggagca cgagatcggc ctgcgcgagg cgctcttctg gagcgtcgtc tggacggtca
     4081 tcgcgctgat cttcaacggc tgggtctact acgagttcgg ctcgcaggcg gggctggagt
     4141 tcctcaccgg ctacatcatc gagcgctcgc tcagcttcga caacatcttc gtcttcatcg
     4201 tcatcttcaa ctacttcgcg gtgcccgccc gctaccagca ccgggtgctg ttctggggca
     4261 tcctcggggc gctgatctcg cgcggcatct tcatcgccat gggcacggcg ctgctcaccc
     4321 gcttcgactg gctgatctac gtcttcggcg ccttcctggt ctacaccggc ttcaagatcc
     4381 tcaaggagaa ggagaccgag gtccacccgg agaacaaccc ggtggtcaag ctgttccagc
     4441 gcctcatgcc gatgaccgcc gagtaccacg gcaagcgctt cttcgtgcgc caggccgggc
     4501 ggcgggtcgc caccccgctg atgctggtgc tggtggtggt ggaggcgacg gacgtggtct
     4561 tcgccgtcga ctcgatcccg gccgtcttcg gggtgacgca gaacccgttc atcgtcttca
     4621 cttccaacat cttcgccatc ctggggttgc gcgcgctcta cttcctgctg gcggggctga
     4681 tgcacaagtt ccgctacctg ggcgtcggcc tcggcctggt gctggtcttc gtcggcgcca
     4741 agatgctgat ccaccactgg tacgtcatcc cgacggtctg gtcgctggcc ctcgtgatgc
     4801 tgatcctgac ggtggcgatc gtggtgtcgc tgctgcggcc ggcctcgccg gacgaggtgc
     4861 cgcacgcgcc ggcgccgggg gagccggcgg acttccagca ctcgcaggcc gcccgcgagc
     4921 tgcacctgag cccggcggcg gacgagccgc cggcccatcc acggggctcc ggcggcgccg
     4981 cctaggccgg ccaccattgc gccgtccccc gcggggcggt tacgatgcgg cgtcctccga
     5041 acgcgacacc agaggggcca ccgatggtca cgatcttcat tccgcgagag cagcgccccg
     5101 gcgagacgcg ggtggcggcg acccccgagt ccgtccgccg catggtcaag caggggctgg
     5161 aggtgatggt ggagcgcggc gccggcaccg ccgccttcta tcccgactcc ggctacgaag
     5221 cggccggcgg ccgcctggtc gacgacgccg ccgcgggctg gagcacggcc gacgccgtct
     5281 tcaaggtcaa cgcaccggag ccgggcgagg gggccgggca gcggccgggg tcgctgctgg
     5341 tcgccttcct cgccccctac cagaacctgg acctggtgcg ccgcctggcc gacgggggga
     5401 tctcctccat cgccatggag ctgatcccgc gcatcacccg ggcgcagtcg atggacgccc
     5461 tctcctcgca ggcctcgatc gccggctaca aggcggtgct catcgccgcc ggccggctgg
     5521 gcaagtactt cccgctgctg atgaccgccg ccggcacgat cccgccggcg cgggtggtgg
     5581 tgatgggggc cggcgtcgcc ggcctgcagg cgatcgccac cgcccgccgc ctgggcgcgg
     5641 tggtcgaggt gtcggacgtc cgcgccgtgg tcaaggagca ggtggagtcg ctcggcggca
     5701 agttcatcga gctgccggag atgccgagcg gcgaggggag cggcggctac gccaaggaga
     5761 tgggcgagga gttcctgcgc cgccagcgcg ccatcgtcaa ggagcggatc gccgcctcgg
     5821 acgtggtcat caccaccgcc ctggtgccgg gcaggcgcgc ccccgtcctg gtcaccgccg
     5881 acatggtgga gggcatgcgc gccggctcca tcatcgtcga cctggcggtg gagcaggggg
     5941 gcaactgcga gctctcccgg ccgaacgagg aggtggtcca ccagggcgtg ctgatcctcg
     6001 gcccctcgaa cctgccgggg acgctgcccc acgacgcgag caacctctac gcgcgcaacg
     6061 tcctgacgct cttccagacg ctctgcaaga aagacggggg gatcgtcatc gacaccgccg
     6121 atgaggtgat cgcgggctcg ctcctcaccc atggcggcaa ggtccaccat gggcccacgg
     6181 cggcgctgct cgaagtcccc gcctgaggag ccgccgagga gagggccggg gcgtcccgtt
     6241 tccccggcct cgccgcactg tccaggaaac ggacctattt cttcggcgag cggccgtgat
     6301 agccgcggac cggaaacgga ttcatgactc gagacgacgg ccacggcccg gcgttcgcgc
     6361 tggtagagaa ttccttgcct caaagcgagg agtcgccgct gtccgtgcgt ctcgccgcgg
     6421 ccgaggcggc gctggagcgc gagcgcgccg aggccccgct cctctgccgg ctgctcctgt
     6481 cccatcctcc cgagcgccgg cccgaggtcg ccgccggcga tccgcgcttc cagacctggg
     6541 gggtcggcga gcggctgctg gcgcggagcc tggagctgct ggagcgggac acggccgagg
     6601 ccgagcacct cgccgtcctg gccctcaccg tggcgcggcg cctcggccgc cactcccccg
     6661 ccctggtgcg cgacctcgag gcccgcgcct gggcctgcgc cggcaaggcg cggctgcggt
     6721 ccggcgacct cgcgggcgcc ggcgaggccc tgcgcgccgc cgccgcctgc ctcgcccagg
     6781 gcactggcga cctgctggtc gaggcgctcc tgctggagtt cgaggccgac gtctgccagg
     6841 cccagggcca ccggggcgag gccgccgccc tgctccacca agcctcctcg cgctacgacc
     6901 aggcggggga caccccgcag tccgcccgcc tggcgcgccg ccgcgaccaa ctcctagacg
     6961 acggcggctc ccggcactcg gcggtgcccg gcctcggcag gaggtgaggg ggccgtcttc
     7021 ccgcagcccc caccaaagca ccacgagcca tcgaagattc cggcacgctg ccgaaggttc
     7081 cggcgccccg tcgaagtctc cgacgtgctg tcggaagcgt ccgacgtgct ggcgaagctt
     7141 ccagcgagct gccggagtct tcgctgggct gtcgacgctt ccggcggcct gccggaatct
     7201 ccgatgggct gccggaacct tccaacgcgc cggcggagct tccgaccggc cggcggagcc
     7261 ttcggcgggc tgccgaaggt tgcaacgcgc cgccggagtc tccggcgcgc tgccgaaggt
     7321 tgcaacgtac ggccgaagct tccggcgcgc cgccggagtc tccgccaaga gaaaagcgcc
     7381 tcccccgatc gcccgcggag cgccggggta aactcttcct catgcgccta ctccccctcc
     7441 tgctgctcgc ggccgtctgc ctccccctgg ccatccctgc ccgcgccgcg gccccgcccg
     7501 ctcccatcgg caccggaggc gcggtgtctt cggccgatcc ggcggcgacc gtggcggggc
     7561 tcgagatgct gcgcgccggc ggcaacgccg tggacgccgc cgtggccacg gccctggccc
     7621 tcgccgtggt ctatccggag gccggcaacc tcggcggcgg cggcttcgcc gtggtcagga
     7681 tgggggagca ggtgtccacc ctcgatttcc gggaggcggc gccggccgcg gcccgccgcg
     7741 acatgtacct ggatccgaag ggggagccga tcccccaggc gtcgttcgtc ggccccctcg
     7801 cttccggcgt gcccggctcc cccgcgggac tgcacgagct gcaccgccgg cacggcaagc
     7861 tcccctggcc ccaggtcgtg gcccccgcca tccgcctggc ccgggccggg ctgcgggtgg
     7921 accgtcatct ccatgaagtg ctggaccaga agaaggaggt cctcgcccgc ttccccgaga
     7981 ccgcccgggt ctggctgcgc gacggcgagg cgccgccggt ggggaccgtc atccccctgc
     8041 cgacgctggc ggcgaccctg tccgcctacg ccgggcaggg gccggcggcc ctcgcctccg
     8101 gtccgctggc cgcggcgctg gaggcgacgt cccggaagca cggcggcatc ctgaccgccg
     8161 ccgacctggc ggcctaccgt ccggtctggc gcgatcccgt gctcttcgag gcgttcggct
     8221 ggcggttcgc ctccatgccc ctcccctcct ccggcggcat catcctgggg cagaccctgg
     8281 gggcgctgga gcggctcgag tgggcgaagc tcccccccct cggcgccgag cgccaccacc
     8341 tcctgaccga ggtctggcgc cgcgccttcg ccgaccgcta cctgctgggc gaccccggca
     8401 ccacccgcgc caccgcggcg cagctcctcg acccggcctg gatcgggaag cgcgcc
//
LOCUS       DASPJI010000442         3909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_835756, whole genome shotgun sequence.
ACCESSION   DASPJI010000442 DASPJI010000000
VERSION     DASPJI010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835756"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VEL74_12010"
     CDS             complement(<1..865)
                     /locus_tag="VEL74_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63299.1"
                     /translation="MRRPKLLPAALLVVAALFTPRLALAEPAPPPPLERLARLGRLWG
                     EVRYLHPYLAYKEEIDWDAALLAAIPKVREAKTREEYAAAAGGMLAALNDPVTRLADA
                     PAPPSAAAPGEGPPSLFERRDDVLVVDLPRFSRAATFMDLYQQSLLFDQELGRSPAAP
                     VVVDLRFGDEPAGIASFIFMDAAERIGATLMSRPCRAPAARYLLHSGYRPQSGLDSGG
                     YFSGFVHQAARNYTPLPGAAPRRVVFLTGAGTRSLPPAVLALQACGDGRIVSAGKLSE
                     EMVASSRRVDLG"
     gene            complement(862..3183)
                     /locus_tag="VEL74_12015"
     CDS             complement(862..3183)
                     /locus_tag="VEL74_12015"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYG63300.1"
                     /translation="MHRKLVPAALVLAFLFALGPPLAAENAAAPAPQSSIERLTHVGK
                     LWGMVRYLHPYIAYKEIDWDAALVAAVPEIREAKTREEYRAAVEGMLAALGDPDTKVM
                     DVPAQPSSTPMSRPGPPEKKPQAPPVVRRLKDGVVAFDLGPYLSAASPRELGPHYQAL
                     RQEIGQARAVVVDLRSSDKAGSYGYAIPGVLEQSASFLAGRRCQGPGERYLMHSGYRP
                     QFGSLTGGYYSGFLTLASNAYGPAPGTAESPKRVVFLVDQGNRSLPNIVSALQACGDG
                     RVVSVGKLAAQSPAETRSFPLGEGLMANVRVSEVLPLPGWNGLAADVELPEGTSPEAA
                     YQAALAEARRPIQDTPRDASAFTPLPDFVLRLDKTYPEMVEPDLPHRQLAVIRAWNVI
                     HHFYPYLHLLDDWEAVLPEFLTRMETAATGRDYAQALLEMMTRVADSHTGVWGHPEMS
                     KDLGNGWLPLEVRWIEEAAVVRAVGEEARPSGILPGDAILAVDGEATAALIERRSRTM
                     PASTPSGRLARICREILRGPAGSTAVLSVQSPGGTPREVRLQRDPRHGAFFPPSEGET
                     VRILPGNLGYADLTRLTIPEVDAMFEKVKDTRALIFDMRGYPHSTAGEIAARINTRKA
                     RIGAQFRRSQVSAFSTEETASGYYFSQPLTEDPRGLPLYTRPTVMLIDDQAISAAEHT
                     GLFLEAANGTRFIGTPTAGANGDITRFTLPGGVNVMFTGHDVRHADGRQLQRVGLIPE
                     VEVAPTRQGLHDGRDEVLERAVRFLEEELGGGK"
     gene            3359..3502
                     /locus_tag="VEL74_12020"
     CDS             3359..3502
                     /locus_tag="VEL74_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63301.1"
                     /translation="MESVQRVESATEARSGGTVEPYYVSSPGELSAALESILDRLRAE
                     VIP"
     gene            complement(3541..3720)
                     /locus_tag="VEL74_12025"
     CDS             complement(3541..3720)
                     /locus_tag="VEL74_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63302.1"
                     /translation="MRKMLIALTLTLTAAAAASTTSAAAATKCPRNTYLVVCSSTLSI
                     CCPNGAACLCPPRES"
BASE COUNT          581 a         1295 c         1483 g          550 t
ORIGIN      
        1 cgccgaggtc gacccgcctg gagctcgcca ccatctcctc ggacagcttg ccggcggaga
       61 cgatccgtcc gtcgccacag gcctggaggg cgagcacggc cggcggcagc gagcgggtcc
      121 ccgcgccggt gaggaagacc acccgcctcg gggcggcgcc ggggaggggc gtgtagttcc
      181 tggcggcctg atggacgaag ccggagaagt agccgccgga gtcgagcccg ctctgcggcc
      241 ggtagccgga gtggaggagg tagcgggcgg ccggcgcccg gcagggccgg ctcatcagcg
      301 tggcaccgat ccgctccgcc gcgtccatga agatgaagga ggcgatcccg gccggctcgt
      361 ccccgaaccg caggtcgacc accacgggag ccgccggcga gcgcccgagc tcctggtcga
      421 agagcaggga ctgctggtag aggtccatga aggtcgccgc gcgggagaag cgggggaggt
      481 cgacgaccag cacgtcgtcc cggcgctcga acagggaggg cggcccctcg ccgggagcgg
      541 cggcggaggg gggcgccggc gcgtccgcca ggcgggtgac cggatcgttc agggcggcga
      601 gcatcccgcc ggccgccgcg gcgtactcct cccgcgtctt cgcctcccgg accttcggga
      661 tcgccgcgag gagggcggcg tcccagtcga tctcctcctt gtaggcgagg tacggatgga
      721 ggtagcggac ctccccccag agcctgccga gccgggccag ccgctcgagg ggcggcggag
      781 gggctggctc ggcaagggcg agccgggggg tgaagagggc ggcgacgacg aggagggccg
      841 ccgggaggag cttcggccgc ctcactttcc gcctcccagc tcctcctcca ggaagcgcac
      901 cgcccgctcc agcacctcgt cgcgcccgtc gtgcagcccc tggcgggtgg gggcaacctc
      961 cacctccggg atcaggccga cgcgctggag ctggcggccg tcggcgtggc ggacgtcgtg
     1021 gccggtgaac atcacgttga cgccccccgg cagggtgaag cgggtgatgt cgccgttggc
     1081 cccggcggtc ggggtgccga tgaagcgggt gccgttggcc gcttcgagga aaaggccggt
     1141 gtgctcggcc gcgctgatcg cctggtcgtc gatcagcatg accgtggggc gggtgtagag
     1201 agggaggccc cgcgggtcct ccgtgagcgg ctgggagaag tagtagccgg aggcggtctc
     1261 ctcggtggag aaggcggaga cctgggaacg ccggaactgg gcgccgatcc tggccttcct
     1321 ggtgttgatc cgcgccgcga tctcacccgc cgtgctgtgc gggtagccgc gcatgtcgaa
     1381 gatcagcgcc ctcgtgtcct tgaccttctc gaacatggcg tccacctccg ggatcgtgag
     1441 ccgggtcagg tccgcgtagc ccaggttgcc cgggaggatg cgcacggtct ccccctcgct
     1501 gggagggaag aaggccccgt gcctgggatc gcgctggagc cgcacctccc gcggcgtgcc
     1561 acccgggctc tggaccgaga gcacggcggt cgagccggcc gggccccgca ggatctcgcg
     1621 gcagatccgg gccaaccggc cggatggagt ggaggcaggc atggtccggc tccgccgctc
     1681 gatcagggcc gcggtggcct cgccgtccac cgccaggatc gcgtcgcccg gcaggatgcc
     1741 ggacgggcgc gcctcctcgc ccaccgccct caccacggcg gcctcctcga tccagcggac
     1801 ctcgagggga agccacccat tacccaggtc cttgctcatc tcggggtggc cccacactcc
     1861 ggtgtggctg tccgcgacgc gggtcatcat ctcgaggagg gcctgcgcgt agtccctgcc
     1921 ggtggcggcc gtctccatcc gggtgaggaa ctcgggcagt accgcctccc agtcgtccag
     1981 gaggtggaga tagggataga agtggtggat cacgttccag gcgcggatca ccgcgagctg
     2041 gcggtgaggg aggtccggct ccaccatctc cggataggtt ttatcgagcc gtaagacgaa
     2101 gtccggcagc ggggtgaagg cggaggcatc ccgcggcgtg tcctggatcg ggcgtcgggc
     2161 ctcggcgagc gccgcttgat aggccgcctc ggggctcgtc ccctccggca gctcgacgtc
     2221 cgcggcgagg ccgttccatc ccgggagcgg caggacctcg ctcacccgta cgttggccat
     2281 cagaccctcg cccaggggga acgaccgcgt ttccgcgggc gattgcgccg ccagcttgcc
     2341 gaccgacacg accctgccgt cgccacaggc ctggagcgcg gagacgatgt tgggcaggga
     2401 ccggttcccc tgatccacca ggaagaccac ccgcttgggg gactccgcgg tgccgggggc
     2461 gggcccgtag gcgttcgagg ccagagtgag gaagccggag tagtagccgc ccgtgaggga
     2521 gccgaactgc ggccggtagc ccgagtgcat gaggtagcgc tcgccgggcc cctggcaccg
     2581 gcggccggcc aggaacgagg cgctctgctc caggacgccg ggaatggcgt agccgtagct
     2641 gccagccttg tcgctggagc gcaggtcgac caccacggcc cgggcctgcc cgatttcctg
     2701 ccgcaacgcc tggtagtgcg ggccgagctc ccgggggctg gcggcgctga ggtaggggcc
     2761 gaggtcaaag gcgacgacgc cgtccttcag ccggcggacc accggcgggg cctgcggctt
     2821 tttctccggc ggccctggcc tggacatggg cgtggaggag ggctgcgccg gcacgtccat
     2881 caccttcgtg tccggatcgc ctagcgccgc gagcatcccc tcgaccgccg ccctgtactc
     2941 ctcccgggtc ttcgcctcgc ggatctccgg gaccgccgcg acgagggccg cgtcccagtc
     3001 gatctccttg taggcgatgt agggatggag gtagcgcacc atcccccaga gcttgcctac
     3061 atgggtgagc cgctcgatgg acgactgggg agcaggagcg gccgcgttct ccgcggcgag
     3121 aggcgggccc agggcgaaga ggaaggcgag gacgagagct gccgggacga gcttccggtg
     3181 catgaggcct ccatgaacgt gtgtgggtgc tcatgatacg tccaccatag gccgaggtta
     3241 cgtcctgcgt cttccccacg gctcgatagg gtgagccgcc aagcgcacga aaggagaaga
     3301 accatgatgg gcaagcttgg tggagttgag gtaggccgcc ttgtaggggg tgccgtagat
     3361 ggagtcggtg cagagggtcg agtcggcgac cgaggcgcga agcggcggca ccgtagagcc
     3421 gtactacgtg agcagccccg gcgagctctc ggccgcgctg gagagcatcc tcgaccggct
     3481 ccgtgccgag gtcatcccct agcgcggcga gagcgacaga cggggcggct cacttcgagg
     3541 tcaagactcc cgtgggggac agagacaggc tgccccgttc gggcagcaga tgctgagggt
     3601 gctgctgcac accacgaggt aggtgttccg cgggcacttg gtggcggctg cggccgaggt
     3661 ggtgctggcg gccgcggcgg cggtgagagt cagggtcagg gcgatgagca tctttcgcat
     3721 aaatgagact cctcaagaaa aaggagggca gtggatgcgc cctccctccc cattctaggc
     3781 gcaaccgtgc ggcgacaggc acggtaatgt cgcatcctgg accggctccg tgccgagatc
     3841 atcactagct cctggagaac gacgccagct cgatgccggc ctcccgcagc atcccgggga
     3901 ggccggggt
//
LOCUS       DASPJI010000443         2691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_197933, whole genome shotgun sequence.
ACCESSION   DASPJI010000443 DASPJI010000000
VERSION     DASPJI010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197933"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VEL74_12030"
     CDS             <1..1089
                     /locus_tag="VEL74_12030"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HYG63303.1"
                     /translation="PSTSARLCLEEVERRTGFRARWKELEEARQGAGDGETRRGIGLS
                     LYFHGAGFTGNGERKMKSPVAARLREDGRIEVLTAMTDMGQGCAVVFPQIAAAAGGIG
                     TDDVVFAEPDTGEVPDSGPTVASRTTMIVGGAIARAVAELRDKVIAGCSQGSQGGEGF
                     SVRDGWIVGPDGPVEPFREAARKLRQTAGPVEVTTRYEPPDWQTFDESTYQGTAYPTY
                     AWGADVVEVEADPDTLAVRPMQVTAVCEVGKAIHPVLCAGQIEGGTLQALGWALMEEV
                     KLQDGRYLNDRLATYIIPTILDSPRMDVHLLERAWEGGPFGAKGVGELPMDGGAPAAA
                     QAIENALGIPVREIPATPEKLLAEVSRT"
     gene            1086..1535
                     /locus_tag="VEL74_12035"
     CDS             1086..1535
                     /locus_tag="VEL74_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYG63304.1"
                     /translation="MKVPFAVNGRAVEIDGHPMERLLDVVREGLGLTGTKEGCGEGEC
                     GACTVLLDGEPVLSCLVPLFQCEGRRVETVEGIAGGPARGFMDRFVATGGVQCGACTP
                     GIAVTAWALLERNPRPGREEVREALAGNLCRCTGYEGILRAFVEPES"
     gene            1535..2419
                     /locus_tag="VEL74_12040"
     CDS             1535..2419
                     /locus_tag="VEL74_12040"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HYG63305.1"
                     /translation="MPETSEIIALRPRSLDEAVRHLGEDPALVPTAGCTDLMVRGPEA
                     LHRMDRVIDLLGIPELRGIQEVDGAIEIGAIEIGATVTFSEIRNSAVVREAFPALVEA
                     AAQIGGWQIQNRATLGGNVANASPAGDSLPVLLALDAVIVAAGPQGLREIPYADFHTG
                     YRKTALRPGEIVARIRLPRPAEGSFQAFRKVGTREAQAISKVVVALAGRVEGDRIAEL
                     RLGAGSVAPVPVRLRAAEDAVRGMAAGPEAADMAGLEAAREVAPIDDVRSTADYRRFA
                     LGRVVRRMVLEMIRRESR"
     gene            2416..>2691
                     /locus_tag="VEL74_12045"
     CDS             2416..>2691
                     /locus_tag="VEL74_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate kinase"
                     /protein_id="HYG63306.1"
                     /translation="MTTTLAAGSVREVFERLRGANLEFNRRHPGESERRQPVHSVYGG
                     AHLFRSDSAARFGALALRALEEHAPTPGDLARAVGLPEELAPRIYERV"
BASE COUNT          371 a          943 c         1014 g          363 t
ORIGIN      
        1 cccagcacct cggcccgcct ctgcctcgag gaggtggagc ggcggaccgg cttccgcgcc
       61 cgctggaagg agctggagga ggcgcggcag ggggctggtg acggcgagac ccggcggggg
      121 atcggcctct ccctctactt ccatggcgcc ggcttcaccg gcaacggcga acgcaagatg
      181 aagtcgccgg tcgccgcccg gctgcgcgag gacggccgca tcgaggtgct gaccgccatg
      241 accgacatgg ggcaggggtg cgccgtggtc ttcccgcaga tcgccgccgc ggccggtgga
      301 atcgggacgg acgacgtcgt cttcgccgag ccggacaccg gcgaggtccc cgactccggc
      361 ccgacggtcg cgtcgcggac caccatgatc gtcggcggcg ccatcgcccg tgccgtggcc
      421 gagctgcgcg acaaggtgat cgccggatgc tcgcaggggt cgcagggggg agagggcttc
      481 tccgtccgtg acggctggat cgtggggccc gacggtccgg tcgagccgtt ccgcgaggcg
      541 gcccggaagc tccggcagac agccgggccg gtggaggtca cgacccgcta cgagccgccg
      601 gactggcaga ccttcgacga gtccacctac caggggaccg cctaccccac ctatgcctgg
      661 ggtgccgacg tggtggaggt cgaggccgat cccgacacgc tggccgtccg tcccatgcag
      721 gtcaccgcgg tctgcgaggt ggggaaggcg atccacccgg tcctctgcgc cgggcagatc
      781 gaggggggca cgctccaggc gctcggctgg gcgctgatgg aagaggtcaa gctccaggac
      841 ggccgctatc tcaacgaccg cctcgccacc tatatcatcc cgaccatcct cgacagcccg
      901 cggatggacg tccacctgct ggagcgcgcc tgggagggcg ggcccttcgg cgccaagggg
      961 gtcggcgagc tgccgatgga cggcggcgcc ccggccgccg cgcaggcgat cgagaacgcc
     1021 ctgggcatcc cggtgcgcga gatcccggcc actccggaga agctgctggc ggaggtatcc
     1081 agaacatgaa ggtccccttc gcggtcaacg gccgtgccgt cgaaatcgac ggccacccga
     1141 tggaacggct gctcgacgtg gtgcgggagg ggctcggcct taccggcacc aaggaggggt
     1201 gcggcgaggg ggagtgcggc gcctgcacgg tgctgctcga cggcgagccg gtcctctcct
     1261 gcctcgtccc cctcttccag tgcgaagggc ggcgggtcga gaccgtcgag gggatcgccg
     1321 gcgggccggc gcgtggcttc atggatcgct tcgtggcgac cggcggcgtc cagtgcggcg
     1381 cgtgcacgcc ggggatcgcg gtcaccgcct gggcgctcct cgagcgcaat ccccggccgg
     1441 ggcgcgagga ggtgcgcgag gcgctggcgg gcaacctctg tcgctgtacg ggctacgagg
     1501 gaatcctgcg cgccttcgtg gagccggaat cgtgatgccg gagacgtcag agatcattgc
     1561 gctccgccca cgctcgctgg acgaggcggt ccgccatctg ggagaggatc cggcgctcgt
     1621 ccccacggcc ggctgcaccg atctcatggt gcgcggcccc gaggccctcc atcgcatgga
     1681 ccgggtgatc gatctcctcg gcatccccga gctgcgcggg atccaggagg tggatggggc
     1741 gatcgagatc ggcgctatcg agatcggtgc gacggtcacg ttcagcgaga tccgcaattc
     1801 ggccgtggtg cgcgaagctt tcccggccct cgtcgaggcc gccgcgcaga tcggcggctg
     1861 gcagatccag aaccgggcca ccctcggggg caacgtcgcg aacgccagcc ccgccggcga
     1921 ctcgctgcct gtcctcctcg ccctggacgc ggtgatcgtg gcggccggcc cccagggcct
     1981 gcgcgagatc ccctacgcgg atttccacac gggctaccgc aagaccgcgc ttcggcccgg
     2041 agagatcgtc gcccgcatcc gtctcccgag accggccgaa ggctcgttcc aggccttccg
     2101 caaggtgggc acccgggaag cccaggcgat cagcaaggtg gtcgtcgccc tggcaggccg
     2161 agtggagggg gaccggatcg ccgagctccg tctcggcgcc ggcagcgtgg cgccggtgcc
     2221 ggtccgcctg cgcgccgccg aagacgcggt gcgggggatg gcggccggcc ccgaggcggc
     2281 ggacatggcg ggcctggagg cggcgcgcga ggttgccccg atcgacgacg tccgctcgac
     2341 cgcggactac cggcgcttcg ccctggggag ggtggtgcgg cggatggtgc tggagatgat
     2401 ccggagagag agccgatgac cacgaccctc gccgcgggct ccgtccgcga agtcttcgag
     2461 cgcctgcgtg gcgccaacct cgagttcaat cgccgccacc ccggcgagtc ggagcgccgg
     2521 cagcccgtcc actcggtcta cggcggcgcg catctgttta gatccgactc cgccgcccgg
     2581 ttcggagcgc tcgccctgcg cgcgctggag gagcacgccc ccacgcccgg ggacctcgcc
     2641 cgcgccgtgg gtctgccgga ggagctggcg ccgaggatct acgagcgggt g
//
LOCUS       DASPJI010000444         4824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_322253, whole genome shotgun sequence.
ACCESSION   DASPJI010000444 DASPJI010000000
VERSION     DASPJI010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322253"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VEL74_12050"
     CDS             <1..663
                     /locus_tag="VEL74_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYG63307.1"
                     /translation="VPYLVDGCQVVGQMPVDLARLRCDFFGGTARKFLRGPRGIGFLY
                     VADRMLERGAYPLGLDMHGATLQGYAGFDLSPDARRFENWEFSYALVLGLGEAARYAL
                     ELGIERVGGRAIENAAWIRERLRETPGIRVADRGRRLCAIVTAEVIRHDAADVVQRLR
                     GCGINVHVSNPEDGDGGHARPVVRISPHYYNTRKELERTVSEIAEIAGRVSGREGHSG
                     ER"
     gene            660..1931
                     /locus_tag="VEL74_12055"
     CDS             660..1931
                     /locus_tag="VEL74_12055"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HYG63308.1"
                     /translation="MIESMQDTTGLASRAPGLRVRVRRVEGAPVMAVRLWLQGGARAE
                     SLPGQALLSGRMLTEGTRRRDWRRIADDAESKGMILSSTGTFECQCLSIDALAKDWEL
                     ALDWTAELLLEPAFPEDRCAWLAKQAAGELESLADQPDIKTAWGFLEHLYTPHPRSRP
                     IHGSVESLLSLTPADCAGLHRRSLESGVIVAVAGVLDEAAVHERLQQLFAGLPAASRP
                     QPEPAAPSGRAERVSTVETEAEDQAHLYVGHITVPRRHPDYAALELLAVVLGSGAGLT
                     GRIPARIREKEGLAYNSYAQTVAGSGLDPGRLLAYVGTSPATVEKAEKGVIEEITRVV
                     EQGITDAELEEARSYLLGREPFRRETARQWADLLVEAEHYGLPLENLELRHAELKHPD
                     RQAVEAATRRHIHPDQLKVTVGLPGEEDGEE"
     gene            1947..2567
                     /locus_tag="VEL74_12060"
     CDS             1947..2567
                     /locus_tag="VEL74_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HYG63309.1"
                     /translation="MWSLLQPSQQTLQDFIARQRREPFSYPEVGSTGNQYMPVGYDHD
                     HNRWQIGQGRAAFEAACAALRRWEMFPRPWTRIEAAGAPTAAGTTVAMMARVYGLWWL
                     NACRIVYVVDEARPVRRFGFAYGTLLAHVERGEELFSVEHHADDTVWYELRAFSLPRF
                     WMARLARPLARGLQRRFQRDSRDAMTRAVAAHPAGAQGQVLNAPVS"
     gene            complement(2534..3874)
                     /locus_tag="VEL74_12065"
     CDS             complement(2534..3874)
                     /locus_tag="VEL74_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63310.1"
                     /translation="MNGTRRCLPFSWVTAILLLAAPALTAQPGGPLPPLFPADNWWNL
                     DVSAAPVDPRSADFIRFIGESTRLHPDFGGDADVSPEIYGMVYVSVPGSQPLVPVTFV
                     EYPDESDAGAPGRPAGYPIPEEARTQAKWIEGGYPGNVDAGGDRHMLIVDRDRRILYE
                     LYHTFWNAGAGRWEAGSGAIFPLDSNARRPEGWTSADAAGLAILPGLVRHDEVFGAGP
                     IRHAFRFTVRATNGHVWPASHTAGSTSGAPPMGTRLRLKASKDISGHPPEVRKIFQTM
                     KTHGLILADNGSDMYISGAHDRRWDNDVLNPAFASLRASDFEVVQLGWRPAATNRCTP
                     ASTRLCLGDGRFQLEAQWRLADGRTGSGQAVAMTGDTGYFWFFDNANVEMVVKVLNGC
                     GTNNRYWVFAGGLTNARVRLVVTDTQRGTVKEYVNPQGAAFQPVQDTGAFNTCP"
     gene            3965..>4824
                     /locus_tag="VEL74_12070"
     CDS             3965..>4824
                     /locus_tag="VEL74_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYG63311.1"
                     /translation="MLEAEILNTAGLMAVAGLLMATSVLFSRASARFGFPVFLLFLAV
                     GMLAGSEGIGGVEFSDYGFSLRLGTLALALILFDGGLNTPVEAFRRSSRPASVLATLG
                     VAGTMAGVAVAGRLLGLTWSQAFLLGAIISSTDAAVVFAVLRASGLQLRGRVGTTLEL
                     ESGLNDPMAVILTTELTLTLAEGRSFSAWSLLEVPLQLAVGAGLGLAFGYAARWLLRW
                     SRLSTGGLYPVFTLGITLLAFGVPTLLHGSGFLGVYLAGVVLGNGDLPYQAGLRRFHD
                     AAAWFGQVSM"
BASE COUNT          643 a         1657 c         1797 g          727 t
ORIGIN      
        1 gtcccctatc tggtggacgg ctgccaggtg gtgggtcaga tgccggtgga cctggcgcgg
       61 ctgcgctgcg acttcttcgg cggcaccgcc cgcaagttcc tgcgcggccc gcgggggatc
      121 ggcttcctct acgtcgcgga ccggatgctg gagcgcggcg cctaccccct gggcctggac
      181 atgcacggcg ccaccctcca ggggtacgca ggcttcgacc tctccccgga cgcccgccgc
      241 ttcgagaact gggagttctc ctacgcgctg gtgctggggt tgggcgaggc ggcgcgttat
      301 gccctggagc tggggatcga gcgcgtcggt gggcgggcga tcgagaacgc ggcctggatc
      361 cgcgagcggc tgcgggagac gccgggcatc cgcgtcgccg accgcgggcg gcgcctctgc
      421 gccatcgtga ccgcggaggt gatccggcac gacgccgcgg acgtggtcca gcggctgcgc
      481 ggctgcggca tcaacgtcca cgtctccaac cccgaggatg gagacggcgg ccacgccagg
      541 ccggtggtcc ggatctcccc tcattactac aacacccgca aggagctgga gaggaccgtg
      601 tcggagatcg cagagatcgc ggggcgggtg tcgggccgcg aaggccattc gggagagcga
      661 tgatcgagag catgcaggat acgacgggac tcgcttcccg ggccccgggg ctgcgggtcc
      721 gggtgcggcg ggtcgagggg gcgccggtga tggcggtgcg cctctggctg caggggggag
      781 cgcgggcgga gtcgctgccc ggccaagccc tgctctcggg gcggatgctc accgagggga
      841 cccgccggcg cgactggcgg cggatcgccg acgacgccga gagcaagggg atgatcctgt
      901 cgagcaccgg taccttcgaa tgccagtgcc tgtcgatcga cgcgctggcc aaggactggg
      961 agctggccct ggactggacg gccgagctgc tcctcgagcc cgcgttcccc gaggaccgct
     1021 gcgcctggct ggccaagcag gcggcggggg agctggagag cctggccgac cagccggaca
     1081 tcaagaccgc ctgggggttc ctggagcatc tctatacccc tcatccgcgg agccggccca
     1141 tccatggctc ggtggagagt ctcctctccc tgacccccgc ggactgcgcc ggcctccacc
     1201 gccggtccct tgagagcggc gtgatcgtgg ccgtggccgg cgtgctggac gaggccgcgg
     1261 tgcacgagcg cctgcagcag ctcttcgccg gcctgccggc ggcgagccgg ccgcagccgg
     1321 agccagccgc cccgtctggc cgtgccgagc gcgtctccac cgtcgagacc gaggccgagg
     1381 accaggctca cctctacgtc ggccacatca cggtgccgcg ccggcatccg gactacgcgg
     1441 cgctggagct gctggcggtg gtgctgggct cgggggcggg cctcaccggg cgcatcccgg
     1501 cgcgcatccg cgagaaggag gggctcgcct acaactccta cgcgcagacc gtggcaggct
     1561 cgggcctcga ccccggccgt ctcctggcct acgtcggcac ctcgccggcg acggtggaaa
     1621 aggcggagaa aggggtgatc gaggagatca cccgggtggt ggagcagggc atcaccgacg
     1681 cggagctgga ggaggcccgt tcctacctgc tcggccgcga gcccttccgg cgcgagaccg
     1741 cccgccagtg ggcggacctc ctggtggagg ccgagcacta cggcctgccg ctcgaaaacc
     1801 tcgagcttcg ccacgccgag ctcaagcacc ccgaccgcca agccgtggaa gcggccaccc
     1861 gccgccacat ccaccccgac cagctcaagg tgaccgtggg gctgccgggg gaagaggacg
     1921 gggaggagta ggcgcgccct caggccatgt ggagcctcct ccagccctcc cagcagaccc
     1981 tgcaggactt catcgcccgc cagcgacggg agccgttctc ctatcccgag gtgggctcga
     2041 ccggcaatca gtacatgccg gtcggctacg accacgacca caaccgctgg cagatcggcc
     2101 aggggcgggc ggcgttcgag gcggcctgcg cagcgctgcg gcggtgggag atgttccccc
     2161 gcccctggac gcggatcgag gcggccgggg cgcccaccgc ggcggggacg acggtggcca
     2221 tgatggcccg cgtctacggg ctctggtggc tcaacgcctg ccggatcgtc tacgtcgtcg
     2281 acgaggcccg gcccgtccgc cggttcggct tcgcctacgg gacgctgctc gcgcacgtgg
     2341 agcgcggcga ggagctgttc agcgtcgagc accatgccga cgacaccgtc tggtacgagc
     2401 tgcgggcctt ctcgctcccc cggttctgga tggcccggtt ggcccgtccc ctcgcccgcg
     2461 gtctccagcg gcggttccag cgggactccc gggatgccat gacgcgggcc gtggccgccc
     2521 atcccgccgg cgctcagggg caggtgttga acgcccccgt gtcctgaacc ggctggaagg
     2581 ccgcgccctg ggggttgacg tactccttga ccgtgccgcg ttgcgtatcg gtgaccacca
     2641 gccgcacgcg ggcgttggtg agcccgccgg cgaagaccca gtagcggttg ttggtcccgc
     2701 agccgttcag caccttcacc accatctcca cgttggcgtt gtcgaagaac cagaagtagc
     2761 cggtgtcgcc ggtcatggcc accgcctgcc cggacccggt gcggccgtcg gcgaggcgcc
     2821 actgggcctc gagctggaag cgcccatcgc cgagacagag gcgggtgctc gccggggtgc
     2881 agcggttggt ggcggcgggt cgccagccga gctgcaccac ctcgaagtcc gacgcccgca
     2941 gcgaggcgaa ggccggattc agcacgtcgt tgtcccagcg ccggtcgtgg gcccccgaga
     3001 tgtacatgtc cgaaccgttg tcggcgagga tcaggccatg ggttttcatc gtctggaaga
     3061 tcttcctcac ctcgggggga tggccggaga tgtccttgct ggccttcaag cgcaggcggg
     3121 tccccatggg cggggcgccg ctcgtcgagc cggcggtgtg cgaggcgggc cagacgtggc
     3181 cgttggtcgc ccgcaccgtg aaccggaagg cgtggcggat cgggccggcg ccgaagacct
     3241 cgtcgtggcg caccagcccc ggcaggatgg cgaggccggc ggcgtcggcg ctggtccacc
     3301 cctcgggccg ccgggcgttc gagtccagcg ggaagatcgc cccggagccc gcctcccagc
     3361 gccccgcgcc cgcgttccag aaagtgtggt acagctcgta gaggatacgg cggtcgcggt
     3421 cgacgatcag catgtgccgg tcgccgccgg cgtccacgtt ccccgggtat cccccttcga
     3481 tccacttcgc ctgcgtcctg gcctcctccg ggatcggata gccggccggc cggcccggcg
     3541 ctccggcgtc gctctcgtcc ggatactcca cgaaggtcac cggcaccagc ggctggctgc
     3601 ccgggacgct cacgtagacc atgccgtaga tctcgggcga gacgtcggcg tcccctccga
     3661 agtcggggtg gaggcgcgtg ctctcgccga tgaagcggat gaagtcggcg ctccgcgggt
     3721 ccaccggcgc ggcgctgacg tccaggttcc accagttgtc cgccgggaac aggggaggca
     3781 gagggccgcc gggctgcgcc gtcagagccg gcgcggcgag caggaggatc gcggtcaccc
     3841 aggagaaggg gaggcatcgc cgggttccat tcatagttgt cgatgagctt agcagagggt
     3901 gagcggggag aagaggggag aggataagat gcgcgggcac gacgggaccc ctggatagaa
     3961 caccttgctc gaagcggaga tcctcaacac tgcgggactg atggccgtcg ccgggctgct
     4021 gatggcgacg agcgtcctct tcagccgggc ctcggcgcgc ttcggcttcc ccgtcttcct
     4081 gcttttcctg gccgtcggca tgctggccgg ctccgagggg atcggcggcg tcgagttctc
     4141 ggactacggc ttcagcctgc gcctcggcac gctcgccctg gcgctgatcc tgttcgacgg
     4201 cggcctcaac acgccggtgg aggccttccg caggagctcg cggccggcct cggtgctggc
     4261 gacgttggga gtggcgggga ccatggcggg ggtggccgtg gcgggacgcc tgctcgggct
     4321 cacctggagc caggccttct tgctgggggc gatcatctcg tccaccgacg ccgcggtggt
     4381 cttcgcggtc ctgcgggcca gcggtctgca gctccggggt cgggtgggca cgacgctgga
     4441 gctggagtcg gggttgaacg atcccatggc ggtgatcctg acgacggagc tgaccctgac
     4501 cctggcggag gggcggagct tcagtgcttg gagcctgctg gaggtgccgc tccaactcgc
     4561 ggtgggggcc ggcctcgggc tcgcgtttgg gtacgcggcc cgctggctcc tgcgctggag
     4621 ccggttgtcg acgggcggcc tctatcccgt cttcactctg gggatcacgc tgctcgcctt
     4681 cggcgtgccg acgctgctcc acggcagcgg cttcctcggg gtctatctgg cgggggtggt
     4741 gctgggcaac ggcgatctgc cataccaggc gggtctccgc cgcttccacg atgccgcggc
     4801 ctggttcggc caggtctcca tgtt
//
LOCUS       DASPJI010000445         3539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_198077, whole genome shotgun sequence.
ACCESSION   DASPJI010000445 DASPJI010000000
VERSION     DASPJI010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198077"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VEL74_12075"
     CDS             <1..506
                     /locus_tag="VEL74_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63312.1"
                     /translation="STHVVADVTGYFTRFPLEQFTTKGDILVVANQPNPTDLSSGTCQ
                     RITTCTVTSPPNTIGKVVIRSMAQVSLNHTQGTHDRVTVGSKLGAEVPPNCTAINDQV
                     ANMDFELPDIHPTSSDVDVTLTHGRDFPQPGGVTRTYSLLTNMIIGASTGDQIESARM
                     ICMFIPD"
     gene            794..2554
                     /locus_tag="VEL74_12080"
     CDS             794..2554
                     /locus_tag="VEL74_12080"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HYG63313.1"
                     /translation="MNRTQRTLSMAVLLVGLAALPAVAADVATAPVAVMSAGSASLDF
                     TTAVEHEALVLTVSGPNDYLFRKEFKAGETPSFSLFNKRGQRLADGHYNYELRVAPVV
                     GKEVRDRLTAAREAGNEKGVLAELRRTGVLPEVTVQSGAFAIANGALMTPGQAEPEPR
                     RARRNKAAGDKAAGIANTFLADQVIPDDLIVEGSGCFGFDCVDGESFGLDTIRLKENS
                     LRIKFEDTSVSPYPTNDWQLTANDSASGGANKFSIEDITGAKVPFTITAGASTNSIFV
                     DSTGRVGFRTATPVLDLHVATSNTPAMRLEQNNSGGFTAQTWDIGANEANFFVRDVTG
                     GSKLSFRIRPGAPTSSIDISADGDVGIGTASPDEKLHIEENVNENTFLVVENTFAGTE
                     AGGFVRAKSDTATLNFQAHSSARTISRFGQVLGGWGELLFAAGNGLILGTNTATPLIL
                     GTNSANRIHIQSGGNVGIGTSTPAHPLQMASGAHVTAGGVWTSVSTRDAKHDIEFLEA
                     EEASKALTTLQPVRFKYNAEPGEEYVGFIAEDVPDLVAANDHKSLNPMDVVAVLTKVV
                     QDQQKTIAELAARVAELEKQ"
     gene            complement(2608..>3539)
                     /locus_tag="VEL74_12085"
     CDS             complement(2608..>3539)
                     /locus_tag="VEL74_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63314.1"
                     /translation="CSILYNLLLSRQDDRPFLLPANICPIVPITFLKARRPFQLVDVA
                     PPGWTLDARQCLDLAPGAAGVLFVRAYGAEHQDPGTLFRALKEQRPDLLVIDDRCLSR
                     PDVDGERLCPWADVSLYSTGRRKHVDLGYGGFAHLALGVPYRRHSAGFRQDQLDAVEA
                     RYLEALERREPFTGGDEEWLDMALPDEPWPHYRERVLDTLAAVDEQKRRLNAIYAAEI
                     PEEARLPAAFQGWRFHVLVPEPDALADRLFAGGLFASRHYQPLAGVFGEGPFPHAEAL
                     HARVVNLFNDFYFDEERARRAAGLVREHLAGRG"
BASE COUNT          618 a         1255 c         1163 g          503 t
ORIGIN      
        1 gctccaccca cgtggtggcc gacgtcaccg gctacttcac ccgcttcccg ctggagcaat
       61 tcaccaccaa gggggacatc ctggtggtgg ccaaccagcc gaacccgacg gacctgtcgt
      121 ccggaacctg ccaacggatc accacctgca ccgtcacctc gccccccaac acgatcggca
      181 aggtggtcat ccggagcatg gcccaggtgt ccctcaatca cacccagggc acccacgacc
      241 gggtcacggt gggctccaag ctcggcgccg aggtccctcc caattgcacg gcgatcaacg
      301 accaggtggc taacatggac ttcgagctgc ctgacatcca ccccacctcg tccgatgtcg
      361 acgtcacgct cacccacggc cgggacttcc cgcaaccggg tggcgtcacc cgcacctact
      421 cgctgttgac caacatgatc atcggggcca gcaccggcga ccagatcgag agcgcccgta
      481 tgatctgcat gttcatcccg gactgacggc gtcccatcct accccctgac ccgaagcccg
      541 gcggtccccc gccgggcttc gggcttcagc ccgcctgtaa cgggttgccc ctccggcccg
      601 cctcttcttg gggtaacccg ggggcaaact gtctcgctct gtgttagtct cggcactcaa
      661 tagtcgattc catgttgttc tagaggatcg ctctgatcct catcctgtct cgaggccggt
      721 gctgctcccg gaggattccg ccggtgtacg gcgggtccaa ccggaaacgt agcaaaagag
      781 ggagtagcaa aagatgaaca gaacgcagcg taccctgtcg atggccgttc tcctggtcgg
      841 gctcgcggcc ctgcctgccg tggcggcgga cgtggccacg gctcccgtgg ccgtgatgag
      901 cgcgggctcg gcgagcctcg acttcaccac ggcggtggag cacgaagccc tcgtcctgac
      961 ggtgagcggg cccaatgact acctgttccg caaggagttc aaggcggggg agacgccctc
     1021 gttcagcctc ttcaacaagc ggggacagag gctggccgac ggccactata actatgagct
     1081 gcgggtcgcg ccggtggtcg gcaaggaggt ccgcgaccgc ctgaccgccg cgcgcgaggc
     1141 gggcaacgag aagggcgtgc tggccgagct gcgccggacg ggcgtgctgc cggaggtgac
     1201 ggtgcagtcc ggcgccttcg ccatcgccaa cggcgccctg atgactccgg gccaggcgga
     1261 gcccgagccg cggcgggcgc gccgcaacaa ggcggccggc gacaaggcgg ccggcatcgc
     1321 caacaccttc ctcgccgacc aggtgatccc ggacgacctc atcgtggagg ggagcggctg
     1381 cttcggcttc gactgcgtcg acggcgagag cttcggcttg gacaccatcc ggctcaagga
     1441 gaacagcctc cggatcaagt tcgaggacac gagcgtcagc ccgtatccca caaacgattg
     1501 gcagctcacc gccaacgact ccgcgagcgg cggcgccaac aagttctcca tcgaggacat
     1561 caccggcgcg aaggtgccct tcaccatcac cgccggcgcc tccaccaact cgatcttcgt
     1621 cgacagcacc ggccgggtgg gcttccgcac cgccactccg gtcctcgacc tgcacgtggc
     1681 caccagcaac acgccggcca tgcggctgga gcagaacaac tccggcggct tcacggccca
     1741 gacctgggac atcggagcga acgaggccaa cttcttcgtc cgcgacgtga ccggcggctc
     1801 gaaactgtcg ttccgcatcc ggcccggcgc gcccaccagc agcatcgaca tctcggcgga
     1861 cggcgacgtc ggcatcggca ccgccagtcc ggacgaaaaa ctccacatcg aagagaacgt
     1921 gaacgagaac accttcctgg tggtggagaa cacgttcgcc ggcaccgaag cgggcggctt
     1981 cgtgcgggcg aagtccgaca ccgccaccct caacttccag gcccacagca gcgctcggac
     2041 aatctcccgc ttcggccagg tcctcggcgg atggggcgag ctcctcttcg ccgccggcaa
     2101 cggcctgatc ctcggcacca acaccgcgac gccgctgatc ctggggacga acagcgccaa
     2161 ccggatccac atccagagcg ggggcaacgt cggtatcggc acctcgaccc cggcgcatcc
     2221 gctgcagatg gcgagcggcg ctcacgtcac ggccggcggc gtctggacca gcgtctccac
     2281 ccgtgacgcc aagcacgaca tcgagttcct ggaggcggag gaggccagca aggcactgac
     2341 gaccctccag ccggtccgct tcaagtacaa cgcggagccc ggcgaagagt acgtgggctt
     2401 catcgccgag gacgtgccgg acctcgtcgc cgccaacgac cacaagagcc tgaacccgat
     2461 ggacgtcgtg gcggtgctca ccaaggtggt ccaggaccag cagaagacca tcgccgagct
     2521 ggcggcgcgc gtcgccgagc tcgagaagca gtagcaggtc tcccaggggc gagggccgcg
     2581 ggtcctcgcc tcaccccctt ctttctccta tccgcggccg gcgaggtgct cgcgcaccag
     2641 cccggcggcc cgccgcgccc tctcctcgtc gaagtagaag tcgttgaaca ggttgaccac
     2701 ccgcgcgtgg agggcttcgg cgtgggggaa ggggccctcg ccgaagacgc cggcgagcgg
     2761 ctggtaatgg cggctggcga acaggccgcc ggcgaacaga cggtccgcca gagcgtccgg
     2821 ctcgggcacc agcacatgga agcgccatcc ctggaatgcc gcggggagcc gggcctcctc
     2881 ggggatctcc gccgcgtaga tcgcgttcag ccgccgcttc tgctcgtcca ccgcggcgag
     2941 cgtgtcgagg acgcgctccc ggtagtgcgg ccagggctcg tcgggcagcg ccatgtcgag
     3001 ccactcctcg tctccgccgg tgaagggttc ccggcgctcc agggcttcga gataacgggc
     3061 ctccaccgcg tcgagctggt cctgccggaa accggcgctg tgacgccggt agggtacgcc
     3121 cagcgccagg tgggcgaatc cgccataccc gagatcgacg tgcttgcgcc ggccggtgct
     3181 gtagagggag acgtccgccc aggggcagag gcgctcgccg tcgacatcgg gacggctgag
     3241 gcaccggtca tcgatgacca gcaggtcggg ccgctgctcc ttgagcgccc ggaagagcgt
     3301 gcccgggtcc tggtgctccg ccccgtatgc gcggacgaag aggacaccgg cggccccggg
     3361 ggccaggtcg aggcactgcc gcgcgtccag ggtccagccg ggcggagcga cgtccaccag
     3421 ctggaacggc cgccgcgcct tcagaaaggt gatgggaacg atggggcaga tgttggccgg
     3481 cagcaggaac gggcggtcgt cctgtcgcga cagcagcagg ttgtagagga tgctgcagg
//
LOCUS       DASPJI010000446        15040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_256722, whole genome shotgun sequence.
ACCESSION   DASPJI010000446 DASPJI010000000
VERSION     DASPJI010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15040)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15040
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256722"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..15004)
                     /locus_tag="VEL74_12090"
     CDS             complement(<1..15004)
                     /locus_tag="VEL74_12090"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63315.1"
                     /translation="MAAVWCEVLGVPQVGSEENFFDLGGHSLLLPQVQALLRDRLGRE
                     VSLLDLLTHTTVRALARHLEPGLVVAEPMPVRADGPRPPGSGSIAIVGLAGRFPGAPG
                     VEALWDNLRAGIASIARFSEEELAASGIPPELRRDPRYVPAAGLLEGVELFDAGFFGY
                     NPREAELLDPQQRVFLECAWEALENAGYDSQRVPGPVGVFASLGFSRYLHQVLNSADP
                     AAAGGLQLLLGNDKDFLSTRVSYKLDLAGPSMTVQTACSSSLVAIHLACQNLRLGACD
                     MALAGGVTIALPQRAGYVYEEGGIVSPDGYCRTFDAEARGTVGGSGVGIVVLKRLEDA
                     LAHGDTIHAVILGSAVNNDGGSSKAGYTAPSVAGQAAVISAALADAGVSPESIAYVEA
                     HGTATPLGDPIEVTALTQAFRRGTDRTGYCLLGSVKTNVGHLDAAAGVTGLIKTALAL
                     EHRQIPPSLHYTAPNPAIDFAASPFRVVDRLIEWPTNGEPRRAGVSAFGIGGTNVHAV
                     LEEAPPVAPSGPSRSWQVLLLAARTPTALERMTEALADRLESDPELSLADAAYTLRVG
                     RRPFAHRRAIVATTREEAVAALRARDPQRVWTAAAARETGGRRSLAFLLPGVGDQYPG
                     LARGLYHEEPVFRQEIDRCAELLLPHVGLDLREVLFAAEGPRAEAPAGHDLRALLGRG
                     DQKGERESAARALLRETRIAQPAMFAVGYALARLWMSWGVRPSALLGYSLGEYTAACL
                     AGVMDLPDALALVARRARLIGELAPGALLAVPLSEEEMRARLAPELSLAAVNAPAVSV
                     VSGPPEAVARLEQRLAGEGVPCRRLQADQAFHSWMMQPVAAELRKMLRAIPLTPPRIP
                     YLSNVTGTWISPAQAMDPDYWVSHLVSPVRFADGVAELWREPGRILLEMGPGQTLGSL
                     ALQQIPTEGAADRTVLSSLRHELDRQADQRFLLQGLGRLWLAGAEIDWSGFHGDERRR
                     RVPLPAYPFERQRYWIEPRIEPVRAGLPAAVPVAFVLPEGAAVDPAWAAALEARGVRL
                     VSLPSGPPTPEAIDELLGLQPQPIAETASAEGAPSSAHIRPGIDTPYEEPRTDVERRL
                     AAIWGELLGIDRVGAHDSFFALGGHSLLGLQLVSRLQAGFGVEVPLRTLFEAPTVAAL
                     ALEVEERRNQGETADVPPLVPMPRHGALSLSFSQQRLWFIDQLQPGTSLYNLPVVLRI
                     EGPLRPAVLERCLGEVVRRHESVRTVFALQGDAPVQVIQLPAPFALPLVDLSGLPEPA
                     REAAALALGEEEAGRPFDLARDLMLRGLLLRLTGEDHALALIMHHVASDGWSMGILVG
                     EVAELYAALSQGEPPSLPELPIQYADFSLWQRSWLHGQVLEREIDWWRRQLAGLPPLL
                     ELPTDRPRPAVQSFRGATRPVRLPAGLTRQAKELGQSEGATLFMVLMATYQAVLARYS
                     GQDDLAVGTPVAGRNRVEVERLIGFFVNNLVLRSDLTGDPTFRELLGRVRETELAAQA
                     HQDVPYEKLVEELAAERSLAHAPLVQVMFALQNTPGGSLEVQDLRLRPVNPEVTSAKF
                     DLTLNLGEYDGGLTGTVEYATDLFAAATVDRLIASFERLLVAATANPDLRVAELPLLS
                     TGECHQVLTEWNDTGEEGWRGSVTSLVERWCRERPDDPAVVDAAGRTLTYGELGERAS
                     RLAGFLRSLGQGPESIVAVLMERSVELLVAQLGVLKAGAAYLSLDPAHPVERLAFMLE
                     ETATPVVLTQEALTDRLAGTTARIIRLDQDLAQGSPMAALPVEPDHLAYVIYTSGSTG
                     RPKGVQVTHRGLLNLVRWDLRAHGTGPGDHRTQVASLGFDASVWEIWSCLASGATLHL
                     PAEEARLDPSRLAAWMAGHGVTDAFLPTPLAETLLAAGGPQIPSLRRLMVGGDRLHLQ
                     PNPGCGFTLVDLYGPAEASVVTSASSLPPRTRGEELEAGGTPTIGRPVDGLRVYLLDR
                     SLQPVPPGVAGELWVGGPALARGYLGDPGRTAERFLPDPWRAGERLYRTGDLCRYRGD
                     GEIVFLGRVDHQVKIRGQRIELGEIEAALAALPGVREAVVVAREGGRGDRHLVAYVTG
                     DAAVDDLRRSLRERLPEAMVPSAFVKLEALPLTPNGKVDRKALPTPERESALEAFLAP
                     RTPVEGVIAGIWAELLGLERVGLDGHFFALGGHSLLAAQVISRLRRAFDIELPVRDLF
                     EAPTVADLAARVEAARRTGAAAAVPPLLPVRREGPLPLSFAQQRLWFIDQLAPGSPLY
                     NMPVVLRAEGPLQPALLRRCLGEIVRRHEALRTVFAVQGDSPVQVIQPPAPFVLPLVD
                     LSGLPEPAREAVALALATREAGRPFDLAGLRGEAMLRGLLLRLAAEDHVVALTMHHIA
                     SDGWSMGILVREVTVLYAAFAQDEPSPLPELPVQYADFSVWQRSWLLGEVLESEIAWW
                     RDQLAGLPPLLELPTDRPRPAVQSYRGATRPVRLPAELTRRAEALGRHEGATLFMVLL
                     AGFQALLARTSGQDDLAVGSPAAGRNRVETEGLIGFFVNTLVLRGNLAGTPTFPELLG
                     RVRETALAAYLHQDVPFEKLVEELAPERSLAHAPLFQVMLVLQNAPVESLEIEDLRLR
                     PAGDTGMMAKFDLTLTLEEGQGGLGGVMEYATDLFDAATIDRLVVHYERLLVAFLATP
                     ERSIWEVPLLSPAERHQAIIEWNDTVVPPAEEVLILDLLAERAEQAPELPAVTQSGEQ
                     LSYGELAASTDRLAAHLRALGVGPDVIVALFLERSIDLVVALLAVLKAGGAYLPLETS
                     LPRPRLSFMFYDARPSLVLTRTRLLPALPELSRVVCLDDLPEIEGAADPAMRPAADHL
                     AYVLYTSGSTGNPKGVAVTHRGLANYLLWAADAYPADEGRGAPVHSPVSFDLTVTSLF
                     LPLLAGRCVDLVPEEEGIEGLAAALTEGGFGLVKLTPAHLEVLQRLLPPERVAGCASA
                     FVIGGEPLSGEQLAFWRDHAPGLRLINEYGPTETVVGCSTYEVPMSMPTAGPVPIGRP
                     IANTRMLLLDRRLEPAPIGVAGELYLGGAGICRGYLRRPDLTAERLVPDPFGTRGERL
                     YRTGDLARRLPDGMVEFLGRTDHQVKVRGFRIELGEIEAALLSLPGVREAVVQAREDA
                     PGDRRLAAYVVADVAAQELRRSLQERLPDYMVPATFVTLAELPLTPNGKVDRKALPAP
                     ERQSAPEMYLAPRTPVEEVLAGIWAEVLGLERVGVDDHFFELGGHSLLATRVMSRLRA
                     AFEVELPVRDLFEAPTVADLAARVEAARRTGAELTVPPLVAVPRQGDLPLSFAQQRLW
                     VIDQLEPGSPLYNMPVALRVEGPLEAGVLALTLGAIVRRHEALRTVLVAPEGKAVQVI
                     QPPAPFILPLVDLSGLPEPALETAALALAEEEAGRPFDLARDLMLRGLLLRLAARDHV
                     VALTMHHVASDGWSMGILVRELSTLYAALAAGRPSPLPELPVQYADFALLQRSWLHGE
                     TLENEIAWWRRQLAGLPPLLELPTDRPRPAVQSFRGATRPLRMPAEISRHAEALGRRE
                     GATLFMVLLAGFQALLARESGQEYLAVGSPVAGRNQVEIEGLIGFFVNTLVLRGDLAD
                     SPTFRELLGRARETALAAYLHQDVPFEKLVEEIAPERSLAHAPLFQTMLVLQNTPVES
                     LEVRDLRLRPVEGTVRTAKFDLTLTLEERAGELAGLVEYATDLFDGTTIDRLILRFER
                     LLAAALVEPDRRVTELPLLGGTENQQILREWNDTAVDHGVPPLLPLLMEEQAAREPRR
                     VAVEQGSRTLTAGELEARANRLAHLLRRHGVRTEDRVAVCCERSLEMVASLLAVWKAG
                     AAWVPLDPDYPAARLAAVLDDARPALLITGPGAPAELPAGTIQCLDLADAELELMEDA
                     GPVEIDITPDQLAYAIYTSGSTGLPKGVLVSHGAIANRLLWMQRLFPLGEADAVLQKT
                     PFVFDASIWEIFLPLLAGARLVLASPGAHREPAVMAREVRERGVTVLQLVPSVLGPFL
                     DEDLHGSPLQRLFCGGEALPAPLCQRVFERLPGVGLCNLYGPTECAIDVTFHPCRPGM
                     EEAIAPLGRPLDNLLVRILDRWGQPVPPGQPGELCAGGIGGSGLARGYLGRPDLTAER
                     FVPDPCAAAPGARLYRTGDLVRQRPDGTIHFLGRIDHQVKIRGVRIELGEVEAALSGL
                     AGVRQAVAMAREGRLVAWVAGDLDADALHALRQGLRERLPDAMVPSAFVVLAELPTTP
                     TGKVDRKALPAPEASAGREGYVAPRSREEEILATVWAQVLRLPRVGVNDNFFELGGDS
                     ILSVQMVARARQAGLGFTMRQVFEHQTVAELARHVTAAETAVAIRAEQGPVTGEVPLT
                     PIQRWFFEQGFADPHHYNQALVLEAPEPVAPAALDRAMAAIVEHHDALRMRFDGLRQE
                     NAPAEPVAPFYQVDLSGLPATRRSEAFERAAAALQAGFDLSRGPLTRLCLFNAGAGEP
                     ARLLWVNHHLVVDGVSWRVLLEDLEAAYRQAALPPKTTSFQEWARRLAEHAGSEALAR
                     ELDYWRETVEVPVSPLPVDFPSGVTSEAGNLVADEATVSFELSAEETSDLLQTVPSVY
                     RSRIEDALLSALVRALADWTGSSRLRVDLEGHGREPLFAGVDDLDVSRTVGWFTSMYP
                     VVLEAGDAGPGEALMSAGERLRAVPGRGIGYGLLRHLDAEAARLLDEAPPAEILFNYL
                     GQAGASSGESSSFRASAASAGPSRSPRAHRTHLLEVGGIVADGRLRISLTYGSRTHRR
                     ETAERLAAAYADALRQLIRHSRESAPAPRPEAEETPWSPLVPIQPLGTGAPLFCVHAL
                     GGEVLAYYRLARELGTDRPFYGIQARPLVGETEAPRLTIEEMAAEYLDAVRSLQPAGP
                     YLLSGYSFGAVVAFEMARQLNLAGEEVALLALLDEGVPAGDAAAEVDTADVVAGIVQY
                     LARRQGRTVEIDAEVLRDLSLDDQLARGLEILGSTEGLGPDVDIPLLRGLAIGWSARA
                     TAIERYRVSAYPGRITLLRSSSVDLAA"
BASE COUNT         2405 a         5369 c         5311 g         1955 t
ORIGIN      
        1 cggcggccag atcgacgctg ctcgaccgca acagggtgat ccttcccgga taggcggaga
       61 ctctgtaacg ctcgatggcc gtcgccctgg cgctccaccc gatcgccaag ccgcgcagca
      121 gcgggatgtc gacatccggt cccagcccct ccgtgctgcc gaggatttcc aggccccgcg
      181 cgagctggtc atcgagggaa agatcgcgca gcacctcggc atcgatctcc accgtccgcc
      241 cctggcggcg ggcgagatac tgcacgatgc cggcgacgac gtccgcggta tcgacctccg
      301 cggccgcgtc gcccgcgggg accccctcat ccaggagggc gagcagcgcc acctcctctc
      361 cggcgaggtt gagctgccgc gccatctcga acgccacgac cgcgccgaag gagtacccgg
      421 acaggaggta ggggccggcg ggctggaggc tccgcacggc atcgagatac tccgccgcca
      481 tctcttcgat ggtgaggcgt ggcgcctcgg tctcccccac caggggtcgt gcctggatgc
      541 cgtagaacgg acggtcggtg ccgagctcgc gggcgaggcg atagtaggcg agcacctcgc
      601 ctccgagcgc gtggacgcag aagagcggtg cccccgtccc gaggggctgg atgggtacca
      661 gcggcgacca gggagtctcc tccgcctcgg gccgcggcgc cggcgcgctc tcccggctat
      721 gccggatcag ttgccgtagc gcgtccgcat aggccgcggc cagtcgctcc gcggtctccc
      781 ggcgatgcgt ccgcgagcca taggtcaagg agatccggag ccggccatcg gccacgatac
      841 cgccgacctc caacaggtgg gtacggtgag cgcgcgggct gcggctgggg ccggccgagg
      901 ccgcggaggc ccggaagctg gagctctcgc cggagctggc gccggcttgg cccaggtagt
      961 tgaaaaggat ctcggctggc ggcgcctcgt ccaggaggcg ggccgcctct gcatcgaggt
     1021 ggcgcagcag gccgtagccg atgccgcgtc cgggaacggc gcgcaaccgc tccccggcac
     1081 tcatcagagc ttcgccagga ccggcgtcgc ccgcctccag gacgaccggg tacatggagg
     1141 tgaaccagcc cacggtccgc gagacgtcga ggtcgtcgac gccggcgaac aacggctcgc
     1201 ggccgtgccc ctccaggtcc acccgcaggc gcgacgagcc ggtccagtcc gccagggccc
     1261 gtaccagggc gctgagcagc gcgtcctcga tgcggctacg gtagacggac ggcacggtct
     1321 ggagcaggtc cgacgtctcc tcggcgctga gctcgaagga gaccgtggcc tcgtcggcga
     1381 cgagattgcc ggcctccgag gtgacgccgg acgggaagtc caccggcaac ggagacacgg
     1441 gaacctcgac ggtctcgcgc cagtaatcca gctcacgggc caatgcctcc gagccggcat
     1501 gctcggccag gcggcgggcc cactcctgga acgaagtggt cttgggaggc aaagcagcct
     1561 ggcggtacgc cgcctcgagg tcttccagca gcacgcgcca ggagaccccg tctacgacca
     1621 ggtggtggtt gacccagagc agccgtgccg gctccccggc tccggcattg aacagacaca
     1681 ggcgggtgag aggtcccctg gacaggtcga agccggcctg gagagccgcg gccgcccgtt
     1741 cgaaggcctc gctccggcga gttgccggca ggcccgagag gtcgacctgg tagaagggcg
     1801 ccaccggctc ggccggcgcg ttctcctgcc gtaggccgtc gaacctcatg cgcagggcgt
     1861 cgtgatgctc cacgatggcg gccatggccc ggtccagagc ggccggggcg acgggctccg
     1921 gcgcctccag cacgagggcc tggttgtaat ggtgggggtc cgcgaagccc tgctcgaaga
     1981 accagcgctg gatcggagtg agcggcacct cacccgtcac cggtccctgc tcggcacgga
     2041 tggcgacggc ggtctccgcg gccgtgacgt ggcgcgcgag ctcggcgacg gtctggtgct
     2101 cgaagacctg ccgcatggta aagcccaggc cggcctggcg cgcccgggcc accatctgga
     2161 cggagaggat cgagtcgccg cccagctcga agaagttgtc gttcaccccc acccgcggca
     2221 ggcgcaggac ctgcgcccag accgtcgcca ggatctcctc ctcgcgactg cgtggcgcga
     2281 cgtacccctc gcgcccggcg gacgcctccg gagccggcag ggccttgcgg tccactttgc
     2341 cggtgggagt ggtggggagc tccgcgagga ccacgaaggc gctgggcacc attgcgtccg
     2401 gcagccgctc gcgcaaccct tgccggagcg catggagcgc gtcggcatcg agatcgccgg
     2461 ccacccaggc gaccagccgg ccctcgcggg ccatcgcgac cgcctggcgc acaccggcga
     2521 ggccggagag cgccgcctcg acctcaccca gctcgatgcg gacgccgcga atcttcacct
     2581 ggtggtcgat gcggcccagg aaatggatcg tgccgtccgg ccgctgccgg acgaggtcac
     2641 ccgtgcggta gaggcgggct cccggcgcag cagcgcaagg atccggaacg aagcgctccg
     2701 ccgtcaggtc gggacgtccg agatagcccc gtgccaagcc actgccgccg ataccgcccg
     2761 cgcacagctc ccccggctgg cccggcggca cgggctgacc ccaccggtcc aggatccgca
     2821 ccaacaggtt gtcgagcggc cggccgagag gcgcgatcgc ctcctccatg cccggccggc
     2881 acgggtggaa ggtgacatcg atggcgcact ccgtggggcc gtagaggttg cagagcccga
     2941 cgccgggcag gcgctcgaac acccgctggc agagcggcgc cggcagggcc tcaccgccgc
     3001 agaacaggcg ctggagcggc gagccgtgca ggtcctcatc caggaacggc cccaggacgg
     3061 aggggacgag ctgaagcacg gtgacaccgc gctcccgcac ctcccgcgcc atgaccgccg
     3121 gctcccggtg cgcgccgggg gacgccagca ccagccgcgc cccggcgagg agcggcagga
     3181 agatctccca gatcgacgcg tcgaagacga agggcgtctt ctggagcacg gcgtcggcct
     3241 ccccgagggg gaagagacgc tgcatccaga gcagccggtt ggcgatggcg ccgtgggaga
     3301 ccagaacccc cttggggagg ccggtggagc ccgaggtgta gatcgcgtac gcgagctggt
     3361 ccggcgtgat gtcgatctct acgggaccgg cgtcttccat cagctccagc tccgcatccg
     3421 cgaggtcgag gcactggatc gtgccggccg gcagctcagc cggagcaccc ggtccggtga
     3481 tcaggagcgc cggccgggcg tcgtccagca cggcggccag gcgcgcggcg gggtagtcgg
     3541 gatccagggg gacccaggcc gcaccggcct tccagaccgc gagcaggctg gcgaccatct
     3601 ccagcgagcg ctcgcagcag acggctaccc tgtcttcggt ccgtacaccg tggcgtcgca
     3661 ggaggtgggc cagccggttc gcccgcgcct ccagctcgcc ggcggtgagg gtacggctcc
     3721 cctgctccac cgcgacgcgc cgcggttccc gcgcggcctg ctcctccatc agcaagggga
     3781 ggagcggtgg aacgccgtgg tccaccgcgg tgtcgttcca ctcgcgcagg atctgctggt
     3841 tctccgtgcc gccaagcagc ggcagctccg tgacgcgccg gtccggctcc accagggccg
     3901 cggcgagcag ccgctcgaac ctgaggatca ggcgatcgat ggtggtgccg tcgaacagat
     3961 cggtggcgta ttcgaccaga ccggccagct cgccggcccg ctcctccagg gtgagggtga
     4021 gatcgaactt cgccgtcctg acggtcccct cgactggccg caggcgcagg tcccggacct
     4081 ccaggctctc gacaggagtg ttctgcagga ccagcatcgt ctggaacaga ggtgcgtggg
     4141 cgaggctcct ttccggggcg atctcctcga ccagtttttc gaacggtacg tcctgatgca
     4201 gataggcggc caatgccgtc tcgcgcgccc ggccgagaag ctcgcggaac gtgggtgagt
     4261 ccgccaggtc cccacgcagg accagcgtgt tgacgaagaa cccgatcagt ccctcgatct
     4321 ccacctggtt gcgtccggcg acgggtgagc ccaccgcgag atactcctga ccgctctcac
     4381 gcgcgagcag cgcctggaag ccggccagga gcaccatgaa gagcgtcgct ccctcgcgcc
     4441 ggcccagggc ctcggcgtgg cggctgatct cggccggcat ccgtagagga cgagtggcgc
     4501 cccggaagct ctgcaccgcc ggccgcggcc ggtcggtggg cagctccagg agcggcggca
     4561 ggcccgcgag ttggcgccgc caccaggcga tctcgttctc cagggtctcg ccatgcagcc
     4621 atgagcgctg caagagcgcg aaatccgcgt actgcaccgg cagctcgggc aacggcgacg
     4681 gccggccggc tgcaagggcc gcatagagcg tcgagagttc gcgcaccagg atgcccatgg
     4741 accagccgtc gctcgccacg tggtgcatgg tgagagccac gacgtggtcc cgcgccgcca
     4801 gacgcagcag caggccgcgc agcatcagat cgcgtgcgag gtcgaaggga cggccggcct
     4861 cttcttcggc gagggcgaga gccgccgtct cgagcgcggg ctccggcagg cccgacaggt
     4921 ccaccagcgg caggatgaag ggtgccggcg gctggatcac ctgcaccgcc ttgccctccg
     4981 gcgcgacgag gaccgtgcgc agcgcctcgt gacggcgcac gatggcgccc agagtgagcg
     5041 ccagcacacc ggcctcgagc ggcccttcga cgcgcagcgc caccggcatg ttgtagagcg
     5101 ggctccccgg ctcgagctga tcgatgaccc agagccgctg ctgggcgaac gacaggggca
     5161 ggtccccctg ccgcggcacc gccacgagag ggggaacggt cagctccgcg ccggtcctgc
     5221 gcgccgcttc gacgcgtgcg gcgagatcgg ccaccgtcgg ggcctcgaag agatcgcgca
     5281 ccggcagctc gacctcgaag gcggcgcgca ggcgcgacat cacccgggtc gccaacagcg
     5341 agtggccccc cagctcgaaa aagtgatcat ccaccccgac ccgttcgaga ccgagcacct
     5401 cggcccagat gccggcgagg acctcttcga ccggcgtgcg cggcgccaga tacatctccg
     5461 gagcgctctg ccgttccggc gccggcaggg ctttcctgtc gaccttgccg ttgggcgtga
     5521 ggggcagctc cgcgagcgtg acgaaggtag ccggcaccat gtagtccggc agccgttcct
     5581 gtaacgaccg gcgtagctcc tgggccgcga cgtcagcgac cacgtaggcc gccagccgcc
     5641 ggtcgccagg ggcatcctcc cgcgcctgta cgaccgcttc gcggactccg ggcagagaga
     5701 gcagcgccgc ctcgatctcc cccagctcga tgcggaagcc cctgaccttg acctgatgat
     5761 ccgtgcggcc caggaactcg accatcccgt ccggcagccg gcgcgcgaga tcgcctgtcc
     5821 ggtagagccg ctctccccgg gtaccgaagg gatcgggtac gagcctctcg gccgtcaggt
     5881 cgggccggcg caggtacccg cggcagatac ccgcgccacc aagatagagc tcacccgcca
     5941 ctccgatcgg agccggctcc agccgccgat ccaggagcaa catccgggta ttggcgatcg
     6001 ggcggccgat gggcaccggc ccggccgtag gcatcgacat cggaacctcg taggtgctgc
     6061 acccgaccac cgtctccgtc ggaccatact cgttgatcaa gcgcaggccg ggagcgtggt
     6121 ctcgccagaa ggcgagttgc tcgccggaca gcggctcgcc accgataacg aacgcactcg
     6181 cgcatcccgc gacccgctcg gggggcagca gccgctgcag gacctccaga tgcgcgggag
     6241 tgagcttgac cagcccgaag ccaccctcgg tgagcgccgc ggccagtccc tcgattccct
     6301 cctcctcggg caccaggtcg acacagcggc cagcgagcag cggcaggaat aggctggtca
     6361 ccgtcagatc gaagctgacc ggtgagtgga ccggagcgcc tcggccctca tccgccggat
     6421 aggcgtccgc cgcccagagc aggtagttcg cgagcccgcg gtgggtgacc gccactcctt
     6481 tgggattgcc ggtcgagccc gaggtataga ggacataagc gagatggtcg gcagccggtc
     6541 gcatggcggg atcggcggca ccttcgatct ccgggagatc gtcgaggcag acgacgcggg
     6601 atagctccgg gagggccggc agcagccgcg tccgggtgag cacaagggat ggacgcgcgt
     6661 cgtagaacat gaacgacagc cgcgggcgcg ggagggacgt ctcgagcggc agataggcgc
     6721 cgcccgcctt gagcaccgcc agcagggcca cgaccagatc gatcgagcgc tcgaggaaga
     6781 gggccacgat gacgtcggga ccgacgccca gagcccgcag gtgagcggcc agacgatcgg
     6841 tgctggccgc cagctcgccg tacgagagct gctctccgct ctgtgttacg gccggcagct
     6901 ccggagcctg ctctgcccgc tccgccagaa ggtcgaggat gagcacctcc tccgccggcg
     6961 gcaccaccgt gtcattccat tcaatgatcg cctggtggcg ttccgccggg gagagcagcg
     7021 gcacctccca gatcgaccgc tccggcgtcg ccaggaacgc gaccaacaac ctttcgtaat
     7081 ggaccaccag ccggtcgatc gtggcggcgt cgaacagatc agtggcgtac tccatcacgc
     7141 cgccgagccc gccctgcccc tcttcgaggg tcagcgtcaa atcgaacttc gccatcatcc
     7201 ccgtatcgcc ggcgggccgc aggcgcagat cctcgatctc caggctttcg acgggagcgt
     7261 tctgcaggac gagcatcacc tggaacagag gagcgtgggc gaggctccgc tccggggcca
     7321 gctcctcgac cagtttttcg aagggcacgt cctgatggag atacgcggcc agcgccgtct
     7381 cgcgcactcg gccgagcagc tcggggaacg tgggcgttcc agccaaattt ccccgcagga
     7441 ccagggtgtt gacgaaaaat ccgatcagtc cttccgtctc caccctgttg cgtccagcgg
     7501 cgggtgagcc caccgcgaga tcatcctgtc cgctggtacg cgcgagaagg gcctggaagc
     7561 cggccagcag caccatgaac agcgtcgctc cctcatgccg gcccagagcc tcggcccgcc
     7621 gggtgagctc ggccggcaac cgtaccggcc gggtggcacc ccggtagctc tgcaccgccg
     7681 gccgaggccg atcagtggga agctccagga ggggaggcag gcccgcgagc tggtcgcgcc
     7741 accaggcgat ctcgctctcc agcacctcgc caagcaacca ggagcgctgc cagaccgaga
     7801 agtccgcgta ctgcaccggc agctccggaa gaggcgaggg ctcatcctgt gcgaaggcgg
     7861 catagagcac ggttacctcg cgcaccagga tgcccatgga ccagccgtcg ctcgcgatgt
     7921 ggtgcatggt gagggcgacg acgtggtcct ccgccgccag acgcaagagc aggccgcgca
     7981 gcatcgcctc gcccctgaga ccggcgagat caaatggccg gccggcctct ctcgtggcca
     8041 gggcgagagc caccgcctcg cgcgcgggct ccggcagacc cgacaggtct accagcggca
     8101 ggacaaaggg agccggcggt tggatcacct gcaccggcga gtcgccctgt acggcgaaaa
     8161 cggtgcgcag agcctcgtgg cggcgcacga tctctcccag gcagcgccgc aggagcgcgg
     8221 gctgcagcgg cccttccgca cgcagcacca ccggcatgtt gtagagcggg cttcccggcg
     8281 cgagctgatc gatgaaccag agccgttgct gggcgaagga cagcggcagg ggcccctcgc
     8341 gccgtaccgg caggagaggg ggaacggccg cggccgcacc ggtccggcgc gccgcttcga
     8401 cccgggcggc gagatccgcc accgtcgggg cctcgaagag atcgcgcacc ggcagctcga
     8461 tgtcgaaggc gcggcgcagg cgcgagatca cctgcgccgc gagcaatgag tgaccaccaa
     8521 gggcaaagaa gtgaccatct agccccactc gctcgaggcc gaggagctcg gcccagatgc
     8581 cggcaatgac cccttctacc ggtgtgcgcg gcgccaggaa cgcctccaga gcgctctccc
     8641 gctccggcgt cggcagggcc ttcctgtcca ccttgccgtt aggggtgagg ggcagggctt
     8701 caagcttcac gaaggcggac ggcaccatgg cttccggcag ccgctcgcgc aacgacctgc
     8761 ggagatcgtc taccgcggcg tcgccggtca cataggcgac caggtgccgg tcgccgcgtc
     8821 cgccctcccg cgccaccacc acggcctcgc gcaccccggg caaggcggcc agcgccgcct
     8881 cgatctcgcc gagctcgatg cgctggccac ggatcttgac ctggtggtcc acccggccca
     8941 ggaacacgat ctcgccgtcg ccacggtagc gacagaggtc tcctgtgcgg tagagccgtt
     9001 cacccgccct ccaggggtcg ggcaggaacc gttccgccgt acggcccggg tccccgaggt
     9061 agccgcgagc gagggcgggc ccgcccaccc acagctcacc cgccacgccc ggcggtaccg
     9121 gctgcagaga ccggtccagc agatagaccc gcaggccgtc gacgggcctg ccgatagtgg
     9181 gggtcccccc tgcctccagc tcttccccac gcgtccgcgg gggcagcgag ctggcgctgg
     9241 tcaccaccga cgcctccgcc gggccgtaga ggtcgaccag ggtgaagcca catcccgggt
     9301 tcggctggag gtgaaggcgg tcgcctccca ccatcagccg gcgcaggctg gggatctgcg
     9361 gtcccccggc ggcgaggagc gtctcggcga ggggagtggg caagaaggcg tcggtgaccc
     9421 cgtgcccggc catccaggca gccagccggg acgggtccag ccgcgcctcc tccgcaggca
     9481 gatggagggt cgcgccggag gccagacagg accagatctc ccagaccgaa gcatcgaagc
     9541 cgaggctcgc cacctgggta cgatggtccc ccggacccgt gccgtgggcc cgcaggtccc
     9601 aacgcaccag gttgagcaga ccgcggtgcg tgacttgcac ccctttcggc cgtcccgtcg
     9661 agccggaggt gtagatcacg taggcgaggt ggtccggttc caccggcaag gccgccattg
     9721 gcgagccctg ggcgagatcc tggtccaggc gaatgatccg cgccgtggtc ccggccaacc
     9781 ggtcggtcag tgcctcctgc gtcagcacca ccggggtcgc ggtctcttcg agcatgaagg
     9841 ccaggcgctc gacgggatgg gcgggatcga gggagaggta ggccgctccg gccttcagga
     9901 cgccgagttg ggccaccagc agctccaccg agcgctccat cagcacggcc acgatggact
     9961 ccggtccctg gcccagagag cgcaggaaac cggccagccg gctggcgcgc tcgccgagct
    10021 cgccataggt cagcgtacgg cccgccgcgt ccaccactgc cggatcgtcg ggccgttcgc
    10081 ggcaccagcg ctccaccagg gacgtcacgg agcctctcca gccctcctct cccgtgtcgt
    10141 tccactcggt gagcacctgg tggcactcgc cggtggagag gaggggcagc tccgcgaccc
    10201 gcaggtccgg attggcggtg gcggccacga gcaggcgctc gaaagacgcg atcaggcggt
    10261 cgacggtcgc ggcggcgaag agatcggttg cgtactcgac cgtaccggtc agcccgccgt
    10321 cgtactcccc gagattgagc gtgagatcga acttcgccga cgtcacctcc gggttcaccg
    10381 gccgcagccg caggtcctgg acctccaggc tcccgccagg agtgttctgc agcgcaaaca
    10441 tcacctgaac caggggggcg tgggcgaggc tcctctccgc ggccagctcc tccaccagct
    10501 tttcgtacgg cacgtcctgg tgagcctggg cagcgagctc ggtctcgcgc acgcggccga
    10561 ggagctcgcg gaaagtgggg tctcccgtca ggtcgctacg cagcacgagg ttgttgacga
    10621 agaacccgat cagcctctcg acttccaccc ggtttcgccc ggcgaccgga gtacccaccg
    10681 cgaggtcgtc ctggccgcta tagcgcgcca gcacggcctg ataggtggcc atgagcacca
    10741 tgaagagcgt cgctccctcg ctctggccca gttccttggc ctgtcgggtg agcccggccg
    10801 gcagccgtac cggccgggtt gccccccgga agctctgtac ggcaggccgc ggccggtccg
    10861 tgggcagctc caggagaggc ggcagaccgg cgagttgacg ccgccaccag tcgatctctc
    10921 gctccagaac ctgcccgtgc agccaggaac gctgccagag cgagaaatcc gcgtactgaa
    10981 tcggcagctc cggcagggac gggggctcac cctgtgacaa ggcggcgtag agctcggcca
    11041 cctcgcccac caggatgccc atggaccagc cgtcgctcgc cacgtggtgc atgatgaggg
    11101 cgagagcatg atcttctccc gtcaggcgga gcagcaggcc acgcagcatc agatcgcggg
    11161 cgagatcgaa cggccggccg gcctcttcct cgccgagggc aagggccgcc gcctcgcgcg
    11221 ccggctccgg cagtcccgac aggtcgacca gcggcagggc gaagggcgcc ggcagctgga
    11281 tcacctgcac cggcgcgtcc ccttgcagcg cgaagacggt gcgcacggac tcgtggcggc
    11341 gcaccacctc tcccaggcag cgctccagca ccgcgggccg caacggccct tcgatgcgca
    11401 gcaccaccgg caggttataa agcgatgtgc ctggctggag ctggtcgatg aaccagagcc
    11461 gctgctgaga gaaggacagg gacagagccc catgtcgcgg catcgggaca aggggcggca
    11521 cgtccgccgt ctcgccctgg ttgcggcgct cctctacctc cagagccaag gcggccacgg
    11581 tgggcgcctc gaagagggtg cgtagaggga cctccacgcc gaatccggcc tggaggcgcg
    11641 agacgagctg taggcccagc agcgaatggc cgcccagcgc gaagaagctg tcgtgggctc
    11701 ccacccggtc aatgccgagc agctctcccc agatcgcggc gagccgccgc tcgacgtcgg
    11761 tgcgcggctc ctcgtaggga gtgtcgatcc cgggccggat atgagccgag ctcggcgccc
    11821 cctcggcgga cgctgtctca gcgatcggct ggggctgcaa gccgagaagc tcgtcgatgg
    11881 cttcgggagt gggggggccc gagggcaggg acaccaaccg gacgccacgc gcctccagcg
    11941 cggcggccca ggccgggtcc accgcggccc cttccggaag cacgaaggcc acgggtaccg
    12001 ccgccggcag gccggcgcgg acgggctcga tccgcggctc gatccaataa cgctgccgct
    12061 cgaacggata cgccgggagg gggacccgcc ggcggcgctc gtcgccgtgg aagccggacc
    12121 agtcgatctc cgcgccggcg agccagaggc ggcccaggcc ctgcagaagg aaccgctggt
    12181 cagcctgccg gtcgagctcg tgtcgcaagg acgagagaac ggtgcggtca gccgcccctt
    12241 cagtcgggat ctgctggaga gccaggctgc ccagcgtctg gcccggtccc atctcgagca
    12301 ggattcgtcc cggctcgcgc cacagctctg cgacgccgtc ggcgaagcgg accggcgaca
    12361 cgaggtggct cacccagtag tccggatcca tggcctgggc cggagagatc caggtccccg
    12421 tgacgttcga gagatacggg atgcgcgggg gcgtcaaggg gatggcgcgc agcatcttcc
    12481 gcaactccgc ggcgaccggc tgcatcatcc aggagtggaa ggcctggtcc gcctgcaacc
    12541 ggcggcaggg caccccctcc ccggcaagcc gttgctcgag ccgggcgacc gcctcgggag
    12601 ggccggacac caccgacacc gccggcgcgt tgaccgcggc gagggagagc tccggagcga
    12661 gccgcgcccg catctcctcc tcggagagcg gcacggcgag cagggcgccc ggcgcgagct
    12721 cgccgatcag gcgggcgcgc cgagccacca gcgccagggc gtcggggaga tccatcactc
    12781 cggccagaca ggcggccgtg tactccccaa ggctgtagcc gaggagggcc gaaggccgca
    12841 ctccccaact catccacagc cgggcgaggg catagcccac ggcgaacatc gccggctggg
    12901 cgatgcgggt ctcccgcagg agcgcccgcg ccgccgattc ccgctcgccc ttctgatcgc
    12961 cacgcccgag cagggcgcgc aggtcgtggc ccgcgggagc ctcggcccgc ggcccctcgg
    13021 ccgcgaacag cacctcgcgc aagtcgagcc cgacgtgcgg caggagcagc tcggcacagc
    13081 ggtcgatctc ctgccggaac accggctcct cgtgatagag gccgcgagcc aaccctggat
    13141 actggtcgcc gaccccaggc agcaggaagg ccaagctgcg acggcccccc gtttcccggg
    13201 cggccgcggc agtccatacg cgctgcgggt cccgcgctct tagcgccgcc acagcctcct
    13261 cgcgcgtcgt tgccacgatc gcacgacggt gggcgaaggg ccgccggccc acgcgcaggg
    13321 tgtaggcggc gtccgccaag ctcagctccg gatcgctctc caggcgatcg gcaagggcct
    13381 ccgtcatccg ttccaaggcg gtgggggtgc gcgccgccag cagcagcacc tgccaggagc
    13441 gtgacgggcc ggagggcgcg acgggcggcg cctcttcgag caccgcgtgc acgttggtgc
    13501 cgccgatgcc gaacgcgctc actccggcgc ggcggggctc cccattcgtg ggccattcga
    13561 tcaggcgatc gaccacccgg aacgggctcg cggcgaagtc gatagccggg ttcggtgctg
    13621 tgtagtgcag gcttggcggg atctggcggt gctccagggc gagcgccgtc ttgatcaggc
    13681 cggtgactcc ggcagccgcg tccaggtggc cgacgttggt cttgaccgag ccgaggaggc
    13741 agtagccggt gcgatcggtc ccgcggcgga aggcctgagt gagagcggtc acctcgatgg
    13801 gatcgcccag aggcgtggcg gtgccgtgcg cctcgacgta ggcgatgctc tctggcgaga
    13861 ctccggcgtc ggcgagcgcc gcggagatga ccgccgcctg cccggcgacg ctgggcgcgg
    13921 tgtagcccgc cttggaggag ccgccgtcgt tgttgaccgc cgagccgagg atcacggcat
    13981 ggatggtgtc gccgtgcgcc agcgcgtctt cgagccgctt gagcacgacg atgccgacgc
    14041 cgctcccccc caccgtgccg cgcgcctcgg cgtcgaaggt gcggcaatag ccgtcgggag
    14101 agacgattcc cccctcttca tacacgtagc ccgcgcgctg cggcaaggcg atggtcacac
    14161 cgccggccag cgccatgtcg caggcgccaa ggcggaggtt ctggcacgcc aggtggatcg
    14221 ccaccaacga gctggagcag gccgtctgca ccgtcatgct ggggcccgcg agatccagct
    14281 tgtaggagac gcgggtggag aggaaatcct tgtcgttgcc cagcaggagc tggagcccgc
    14341 ccgccgccgc gggatcggca ctgttgagga cctgatgcag gtagcggctg aaccccaggc
    14401 tggcgaacac gcccaccggc cccggcacac gctgggagtc gtagccggcg ttctccaggg
    14461 cctcccaggc gcactcgagg aatacgcgct gctggggatc caggagctcg gcctcgcgag
    14521 ggttgtagcc gaagaagccg gcatcgaaca gttccacccc ttcgagcaat cctgcggccg
    14581 gcacgtagcg cgggtcgcgc cgcaactcgg gagggatgcc ggaggcggcg agctcttctt
    14641 cgctgaagcg ggcgatggag gcgatcccgg cgcgcaggtt gtcccacagg gcctcgacgc
    14701 cgggcgcgcc ggggaagcgg ccggcgagtc cgacgatggc aatgctgcca ctgcccggtg
    14761 gccgcggtcc gtcggcacgc accggcattg gctctgctac caccagaccg ggttccaggt
    14821 ggcgcgccag ggcgcggacg gtggtgtggg tcaggaggtc cagcagactg acctcccgtc
    14881 ccagccggtc acgcaaaagc gcctgcacct gggggagcag cagcgagtgg ccgcccaggt
    14941 cgaagaagtt ctcctcgctg cccacctgcg gcacaccgag gacctcgcac cagacagcgg
    15001 ccacggcgtc ctcgagcgcg ctacggccca cctcaggcag
//
LOCUS       DASPJI010000447         4612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_596443, whole genome shotgun sequence.
ACCESSION   DASPJI010000447 DASPJI010000000
VERSION     DASPJI010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596443"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..998)
                     /locus_tag="VEL74_12095"
     CDS             complement(147..998)
                     /locus_tag="VEL74_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63316.1"
                     /translation="MMAWAEMEHRSGVLPLLAAFFAAPEPFAEVIAGRTRGALGLFTP
                     APEPGWGAAALAAEPVGGGALLHGEVRLPGAEAGGSIVLVRLPDAEYRLAWVSHDAPG
                     VERRASRTGGPVPASGEGPCWLHLEGVFVGAGLLSRPVTLEPDGELARHLVSYAGVWA
                     ATAAHCAREGCRALRRAARTAGFHTSQLVALGITEVEIEADLTAAAVRRAVPVDTGRL
                     AVAAAAARTLSAVAAKAEEMRDVYGLDIDGPLAGAAAKSLIAFLGGPLLLENELARAL
                     GIQGAHA"
     gene            complement(995..1270)
                     /locus_tag="VEL74_12100"
     CDS             complement(995..1270)
                     /locus_tag="VEL74_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HYG63317.1"
                     /translation="MNEDDLAAMKKRVQRLVYMAAGGKIQEEDLGHHDTDLRTIGLDS
                     LGYLNLLEGLERTFGVSIDLDQEEDHSFLHTIDNIVRHLLARRGEAA"
     gene            complement(1267..2733)
                     /locus_tag="VEL74_12105"
     CDS             complement(1267..2733)
                     /locus_tag="VEL74_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HYG63318.1"
                     /translation="MSGRSIIRDALRDCLDRHRRRPAVAGGSEALSYEELGRRIEAAA
                     PLLPAGHVGFCLANRPEYVILYFATLVAGHLPLLLDANFNTGEIEAIRGDCGLDALVM
                     ERTKAETLDLPGKGEPIPYGDEVVVLPLARDAGPAFAPRPDTEVCRFTSGTTGRPKCL
                     EFSGHAVVSAARNWVLGTAMTVDDRTLCLAALSNGLAFNTSLLSTFLAGGELCFLKGV
                     PLTRPVARRIAESGITRLVAFPTLYRNFAAPGGPDPSLLAGLKHAISAGAPLWPDVRE
                     EFRALYGLDISDYYGIAETGPCTFERDADHHAGLGQPLPGVEIRIAPGAEDAPGTGEV
                     LVRTASMTSGYLNHPGLFEARVDAEGFYHSGDRGRMIDGRLHLVGRTQDHINVAGRKI
                     DPTEIIAVVSALDGVADAVAFPDEDLNREALVHLVVVASTQEVTRSAVAEACRARLAP
                     YKVPGRISFVQEIPRNGIGKPRIALLREQLGTRKETVA"
     gene            complement(2730..3914)
                     /locus_tag="VEL74_12110"
     CDS             complement(2730..3914)
                     /locus_tag="VEL74_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYG63319.1"
                     /translation="MERSLLDHPALARHFTPEHRELRSRARSYFDPARLPDWVGPGGY
                     LTKAQWEELGRQELLGVSLPRELGGQGLGLLGALILSEAASGLGDLGVSLGMHCQTEI
                     TAHWLATAHAPEVRDRYLPGMIAGRLVGCACDTEPGPQEGQRERQVESTAVRDGDELV
                     VHARKLYVVNGANADLCFVTLKAAGEMATVLVEKERPGVHVLEVFDKLGTRSIDQARL
                     EFDGVRVPAANLSTKRGVRQLMHWNKVMTAPRFLMCADACFLHRRLLDRMLEYGGRRI
                     VEGRPLAAWPINRQALARAAADQELMQAGLLDAFEQLESQRNAVAEIAALKWFCVDRT
                     ARFAALCAELHGGAGYMWTSEFLFAQAQILGLKMAGGSLITMKSIAGQALAYREELEA
                     LA"
     gene            complement(3914..>4612)
                     /locus_tag="VEL74_12115"
     CDS             complement(3914..>4612)
                     /locus_tag="VEL74_12115"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HYG63320.1"
                     /translation="AGAPLLVEDPEVPADSFLPLPEARPPLSAAAEWRSAILGRRSAK
                     GFRQEVVSVAQIGELLGALRATGLTADCSREGSVRLGVRLVARNVEGLAGVFAYLPQG
                     HALQRLEAQAADPRPACMQQGLAGSAAAVLVFHAPLSALIRRHGYSAFAELHFRAAEL
                     GQRLHLAATRLGRLGLTCVGGFDGEECASLAKLDAGEEALYAILIGVPDESVFKHDRL
                     NVAFSHGYTNTLED"
BASE COUNT          698 a         1664 c         1616 g          634 t
ORIGIN      
        1 cgtcacctcg gagagcgccc ggcgcgtcgt cacgccggcg cgcagggggc ggttgcgggt
       61 gaagcggtcg acgacctcga caccgcggga gatggcgacc tcttcgacgg cgcgccccat
      121 cttgccgtaa ccgaccagag ccaccttcat gcgtgcgccc cctggattcc gagggcgcgc
      181 gcgagctcgt tctcgagcag cagcggcccg ccgaggaagg cgatgaggct cttcgcggcg
      241 gctccggcga gcggaccgtc gatgtccagg ccgtacacgt cgcgcatctc ttcagccttg
      301 gcggcgacgg cgctcagcgt ccgggcggcg gcggcggcca cggcgagacg gccggtatcg
      361 accggtacgg cgcgtcgcac ggcggcggcg gtcaggtccg cctcgatctc cacctccgtg
      421 atgcccaggg ccaccagttg cgacgtgtgg aagcccgccg tcctcgcggc ccgccgcagc
      481 gcccggcacc cttcgcgggc gcagtgcgcc gcggttgcgg cccagacgcc ggcgtaggac
      541 acgaggtgcc gcgcgagctc gccatccggc tccagagtca ccggccgcga aagcaggccg
      601 gccccgacga agaccccctc gagatgcagc cagcagggcc cctccccgga agccgggacc
      661 ggtcctcccg tccgcgaggc gcgccgctcg acgcccggcg cgtcgtggct cacccacgcc
      721 aggcgatact cggcgtccgg cagccgcacc agcacgatgg agccgccggc ctccgctccc
      781 ggcagccgta cctccccgtg cagcagcgct cccccaccga cgggctcggc ggcgagcgcc
      841 gcggctcccc agcccggctc gggcgcgggc gtgaagaggc cgagcgcgcc gcgcgtccgc
      901 cccgcgatca cctccgcgaa tggctccggc gccgcgaaga acgccgcgag cagggggagc
      961 acgccggagc gatgctccat ctcggcccac gccatcatgc cgcttcgccc cgccgggcca
     1021 ggaggtgacg cacgatgttg tcgatggtat ggaggaagct gtggtcctct tcctggtcga
     1081 ggtcgatgga gacgccgaac gtccgctcca gcccctccag caggttcagg tagccgagcg
     1141 aatcgagccc gatggtccgc agatcggtat cgtgatgccc gagatcctcc tcctggatct
     1201 tgccgcccgc ggccatatag accagtcgct ggacccgctt cttcatcgcg gccagatcgt
     1261 cttcgttcat gccaccgttt cctttctcgt gccgagctgc tcgcggagca gggcgatccg
     1321 cggcttgccg atcccgttac gcggaatctc ctggacgaaa ctgatgcggc ccgggacctt
     1381 gtagggagcg agccgcgcgc ggcaggcctc cgccaccgcc gagcgggtga cctcctgcgt
     1441 agaggcgacg accacgaggt gaaccagcgc ctcccggttc aggtcctcgt cgggaaaggc
     1501 gacggcgtcc gccactccgt cgagtgccga gaccacggcg atgatctccg tgggatcgat
     1561 cttgcgcccc gccacgttga tgtgatcctg ggtccggccc accaggtgca ggcgtccgtc
     1621 gatcatcctt ccgcggtcgc cggaatgata gaaaccttcc gcgtccacac gcgcctcgaa
     1681 cagccccggg tggttgagat atccgctggt catcgaagcc gtgcgcacca gcacctcgcc
     1741 ggttcccggc gcgtcttcag ctccgggagc gatccggatc tccacgcccg gcagcggctg
     1801 gcccaacccg gcgtgatgat ccgcatcccg ctcgaaggtg cagggaccgg tctcggcgat
     1861 gccgtagtag tcggagatgt cgaggccgta aagtgcccgg aactcctcgc gcacgtccgg
     1921 ccagagcggc gcgccggccg agatcgcgtg cttcaggccg gccaggagcg acgggtccgg
     1981 gccgcccgga gccgcgaagt tgcggtagag ggtgggaaag gcgaccagcc gggtgatccc
     2041 ggactccgcg atgcggcgcg ccaccggacg ggtcagcggc acccccttga ggaagcacag
     2101 ctcgccgccg gccaggaagg tcgagaggag ggacgtgttg aacgccagac cgttggagag
     2161 agcggccagg cagagcgtcc ggtcgtcgac tgtcatcgcc gtaccgagca cccagttgcg
     2221 cgccgccgag accacggcat ggccgctgaa ctccaggcat ttcggccgtc ccgtggtgcc
     2281 ggatgtgaac cggcatacct cggtatcggg ccgcggcgcg aaggcaggcc cggcgtcgcg
     2341 ggcgaggggc aggacgacga cctcgtcgcc gtatgggatc ggctccccct tccccggcag
     2401 gtcgagcgtc tcagccttcg tccgctccat gaccagagcg tcgaggccac aatcgccacg
     2461 gatagcctcg atctcgccgg tgttgaagtt ggcgtcgagc aggagaggga gatggccggc
     2521 aacgagcgtc gcgaagtaga ggatcacgta ctccggccgg ttggcgagac agaagcccac
     2581 gtggcccgcg ggcagcaggg gcgccgcggc ctcgatgcgg cggccgagct cttcgtagga
     2641 cagcgcctcg gagccccccg ccaccgccgg gcggcgtcga tggcgatcga gacagtcgcg
     2701 cagcgcgtcc cggatgatcg aacgcccgct cacgccagcg cctccagctc ctcgcggtag
     2761 gcgagcgcct ggccagcgat ggatttcatc gtgatcaggc tcccgccagc catcttgagg
     2821 ccgaggatct gcgcctgggc gaaaaggaac tcgctcgtcc acatgtagcc cgcaccgccg
     2881 tggagctcgg cgcacagcgc ggcgaagcgg gctgtccggt cgacacagaa ccacttgagc
     2941 gcggcgatct cggccacggc attgcgctgg ctctcgagct gctcgaaggc gtcgagcagg
     3001 cccgcctgca tcagctcctg gtccgccgcg gcgcgagcga gggcctgccg gttgatcggc
     3061 caggccgcga gcggcctgcc ttccacgatg cgccggccgc cgtactccag catgcggtcg
     3121 agcaggcggc gatggaggaa gcaggcgtcg gcgcacatca ggaagcgcgg cgcggtcatc
     3181 accttgttcc agtgcatgag ctggcggacg ccccgcttgg tagagaggtt cgccgctggc
     3241 acccggacac catcgaactc gagcctggcc tgatcgatgg agcgggtccc gagcttgtcg
     3301 aacacctcga ggacgtggac gccggggcgc tccttctcca ccagcacggt cgccatctcg
     3361 ccggcggcct tcagcgtcac gaagcagaga tccgcgttgg cgccgttgac gacgtagagt
     3421 ttgcgggcgt ggaccaccag ctcgtcacca tcgcgcaccg ccgtcgattc cacctgccgc
     3481 tccctctggc cttcctgcgg gccgggctcg gtgtcacagg cgcagccgac gaggcggccg
     3541 gcgatcatcc cggggaggta gcggtcccgg acttccggcg cgtgcgcggt ggcgagccag
     3601 tgagcggtga tctcggtctg gcagtgcatg ccgagggaga cgccaaggtc cccgagcccc
     3661 gacgccgctt cgctcaggat cagcgcgccc agcaggccga ggccctggcc gcccagctcg
     3721 cgcgggagag agacgcccag gagctcctgg cggccgagct cctcccactg cgccttcgtc
     3781 aggtagcctc ccgggcccac ccagtcgggc agccgcgccg ggtcgaaata gctccgcgca
     3841 cggctccgca gctcccggtg ctcgggcgtg aagtgccgcg ccagggcggg atggtcgaga
     3901 agcgagcgct ccatcagtct tccagggtgt tggtgtatcc atggctgaac gccacgttca
     3961 gccggtcgtg cttgaacacc gactcgtcgg ggactccgat gaggatcgcg tagagggcct
     4021 cctcgccggc atcgagcttg gccagagagg cgcactcctc gccgtcgaag ccgccgacgc
     4081 aggtgaggcc gagccgtccg agccgtgtcg ccgccaggtg gagccgctgg cccagctccg
     4141 cggcccgaaa gtgcagctcg gcgaaggccg aatagccgtg ccggcggatc agcgcgctga
     4201 ggggagcgtg gaagaccagc accgccgcgg cgctgccggc gagcccctgc tgcatgcagg
     4261 ccggccgggg gtccgccgct tgcgcctcga gccgttggag ggcatggccc tgcggcaggt
     4321 aggcgaagac tcccgcgagt ccctcgacgt tcctcgccac caggcgcaca cccagccgca
     4381 ccgagccctc ccgggagcag tccgcagtga gcccggtggc gcgtagcgcg cccagcagct
     4441 cgccgatctg cgccacgctc acgacttcct gacggaagcc tttggccgag cgccggccga
     4501 ggatcgccga gcgccactca gccgccgccg acagcggtgg acgggcttcc ggaagcggca
     4561 ggaaggaatc cgccgggacc tccgggtcct cgaccaggag cggagcaccc gc
//
LOCUS       DASPJI010000448         9187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_660893, whole genome shotgun sequence.
ACCESSION   DASPJI010000448 DASPJI010000000
VERSION     DASPJI010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9187)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9187
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660893"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /gene="selA"
                     /locus_tag="VEL74_12120"
     CDS             complement(<1..1063)
                     /gene="selA"
                     /locus_tag="VEL74_12120"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HYG63321.1"
                     /translation="MLEAAMISPDSSDSPDSPDFPGDARRGIPAIDRLLGDPALRALI
                     SLYGRDPVRITARRVVDGLRERWTAASPPENERESTVAGLPGRIAAEVEAALGGPLRR
                     VLNATGLFLHTNLGRAPLPREVAASLPPLLDAYCDLEFDLETGRRSQRLRRVERLLTA
                     LTGAEAAAVVNNNAAALVLILSTLAQGREVVVSRGELVEIGDSFRIPEIMAAAGARLA
                     EVGATNRTRPADYRNAAGPETALLLKVFPSNFRLTGFVASVSPAELVAVGREVCVPVL
                     VDEGSGLLRRHPAPQLAGHPSMTELVAAGCDLVCGSGDKLPGGPQAGLIVGREALVTR
                     CRRHPLYRALRPDRLALAAL"
     gene            1076..1771
                     /locus_tag="VEL74_12125"
     CDS             1076..1771
                     /locus_tag="VEL74_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63322.1"
                     /translation="MDPKRTPRPGAGPAPTLKPGPAKPGAAPGVPKRPGAGPATPQPP
                     GRRGTTSYKEPLDRLATDIRQLQIEFERFFAGALPIPPEDLRNRVQAQLRTLRNATIT
                     AAVDSFRLGDLEARFNTYNELFNRRLREREEGRHRAPRPAPVEVQRFDPLKGVVVGAD
                     VDPEAAEALYQGLASRPASTPRFDLDAFRTYLSRQVSALQAKTGCAEVQFRLAEEEGT
                     LKLKARPVNRQGS"
     gene            1796..2836
                     /locus_tag="VEL74_12130"
     CDS             1796..2836
                     /locus_tag="VEL74_12130"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG63323.1"
                     /translation="MRSRTIRLTAFSLAAVLVAVLALSAIAAAPAPPNIGKALEEQRR
                     LAAERPQDPAVFNDMGNLLMLAHRPADAETAYRRAVELDPAKVSALFNLGLLLQQRGE
                     DREALSLFERVVKQDERYAWAHYQIGTIRESRGQEAGAIDAYAAAFSLDPQLAFSDVN
                     PHIVENRLVTQAMLRAYKADRELPHAPKFYDDPNRIAALLVPPPAPRTPTSDEEGTTQ
                     QAAQAQPGAQTRPGGQELNRNDLQPGRRMGQPQPPGMGGRPAPGGSVRGGYVPPGGYT
                     PPDPNVPRGLRQWNRPEPQTEVNPGEGMQPGQVITPPPGGVYYQPGLPSTGRLDIRLL
                     PGSKPRDREARS"
     gene            3058..3705
                     /locus_tag="VEL74_12135"
     CDS             3058..3705
                     /locus_tag="VEL74_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63324.1"
                     /translation="MKALNLRKSYLVGLAILALAVTAIFINTSGLSAQSSEDEYHTLL
                     GYHGYQSPGGVIFESASTESISANVEEKYLDRKGLATRELNVRGMAGRGYSEGLGETM
                     YWLDKSRPVKGSVVRAKTPGAEYPAVHEMHFHLLLVSDIFPGKTLRSINPAIMVNNNA
                     TSFPPKPGARYQLKNVVELEDVENPGEVMFRIVSNRNQIIGAGRLPLDPAMEPQR"
     gene            3880..4704
                     /locus_tag="VEL74_12140"
     CDS             3880..4704
                     /locus_tag="VEL74_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63325.1"
                     /translation="MIRSTRLIVAISALALALAVVPAQALDNVIYNGSDLWMTPADGS
                     TYAEFKSNPIPAGFFCNKSEPFTGRIAFKGAPLATADGSLGNTDTIVQRLDDAVFNKR
                     GIASTRIQVRALQFESLAPVKTACGSFRVFVRLQGEQPVTRMKIVRESEDGGRFFSPI
                     AVKVRMSFVPVDGKGRERLEVTRSLRFKPSDHAFWSVPSEQALEKRGVALVDTDSDNR
                     PDTYVPGTSNFAAGRRASQNKAIQQDDSVGILHDGYEHYHYTTAAESTTGPTAGAN"
     gene            4871..5695
                     /locus_tag="VEL74_12145"
     CDS             4871..5695
                     /locus_tag="VEL74_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63326.1"
                     /translation="MLRKTRLMVSMAALALVLAVVPAQALDNVIYNGSDLWMTPADGS
                     TYAEFKSNPIPAGFFCNKSEPFTGRIAFKGAPLATADRSLGNTDTIVQRMDDAVFNKR
                     GVASTRIQVRALQFESLAPVKTACGSFRVFVRLQGEQPVTRMKIVRESENGGRFFSPI
                     AVNVRMSFVPVNGKGRERLEVTRSLRFKPSNHAFWAVPTEQAIEKRGVALVDTDSDGR
                     PDTYVPGTSNFAAGWRASQNKEILSSEAERSAVLHDGYEHYHYTYPSETADTQIYN"
     gene            complement(6166..6738)
                     /locus_tag="VEL74_12150"
     CDS             complement(6166..6738)
                     /locus_tag="VEL74_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63327.1"
                     /translation="MHGYPKGTTVPNPPHHPNAFTVAFLSRLAERDEPPTAGDADVAG
                     PWRIEPIPGHGFGLFRVGESLARGFAPAAVFPSRWLALVCAAILPGTGRDPLLLLSKQ
                     PDAHGAYPLALAGGGSGDGGGDDEDGGPVGFFPLFEERAVDAMNVLVHLLRSPEALAF
                     VLEAAGAVALERCGAILDERVAADMAANRE"
     gene            7150..7839
                     /locus_tag="VEL74_12155"
     CDS             7150..7839
                     /locus_tag="VEL74_12155"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HYG63328.1"
                     /translation="MQRIAGIDGCQSRWLAITHEPGTRSFVPQVLETEELPGQPWALA
                     AIDIPIGLPDRGSREADRAARKFVGPRASSVFPCPIRPALDGLSWEAACEITYQINGH
                     RISRQTFAILSKIRAVDECIRSTNLQKRLFEVHPEVSFASWNGAPTLLPKKDARGHEQ
                     RRTLIARHFGPEAFASVVAQIGERSVAADDIADAFAALWSAERILNGTAQRLPSDSVS
                     DSYSLPMHIWY"
     gene            8050..8298
                     /locus_tag="VEL74_12160"
     CDS             8050..8298
                     /locus_tag="VEL74_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63329.1"
                     /translation="MTTTLAYLERLSREIHKPEAEIMTMAFEAGLRQLWREHILGQYL
                     RGEITREKAIESVGIDWVDLAERQRVATNEDLDWALNT"
     gene            8280..8795
                     /locus_tag="VEL74_12165"
     CDS             8280..8795
                     /locus_tag="VEL74_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HYG63330.1"
                     /translation="MGSEHLIAVADAGPLIHLAEIDCLSLLAVFPELHVPEGVWLEVE
                     RPSSIRTELTFAKRHVLAQDEVQKFTAEHGLGKLQAGERESLLLCVRISASVLLTDDL
                     AVRRAAKDLALTPVGSLGVIARAHRMGRISAEVAERLLRQLDTASSLYVTPAIVDLAI
                     ERLRDEGSSKS"
     gene            complement(8805..>9187)
                     /locus_tag="VEL74_12170"
     CDS             complement(8805..>9187)
                     /locus_tag="VEL74_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HYG63331.1"
                     /translation="APIEQEIGQLSPEEQKDFLSDLGLTEPSLDRVNRASYELLGVIS
                     FFTVGEDEVRAWTIRRGNKAREAAGAIHSDIERGFIRAEVVHCDDLIRLKTLAACKDA
                     GLLRLEGKEYVMQDGDVAHFRFNV"
BASE COUNT         1550 a         3027 c         3102 g         1508 t
ORIGIN      
        1 cgagcgccgc cagcgccagc cggtcagggc ggagcgcccg gtagagcgga tggcgccggc
       61 agcgcgtgac cagcgcctcg cgtcccacga tgaggccggc ctggggtccg cccggcagtt
      121 tgtcgccgct gccgcagacc aggtcgcagc cggccgcgac cagctcggtc atgctggggt
      181 ggccggcgag ttgcggggca gggtggcggc gcagcaggcc gctcccctcg tccaccagca
      241 cgggcacgca cacctcccgg cccaccgcga ccagctccgc gggggagacc gaggcgacga
      301 agccggtaag ccggaaattg ctggggaaca ccttgaggag cagggcggtc tccgggccgg
      361 cggcgttccg gtagtcggcg gggcgggtgc ggttggtggc ccccacctcg gccaggcggg
      421 caccggcggc ggccatgatc tcggggatgc ggaacgagtc gccgatctcc accagctcgc
      481 cgcgggagac caccacctcg cggccctggg cgagggtaga gaggatgagc accagggcgg
      541 cggcgttgtt gttgaccacg gcggcggcct cggcgccggt gagggccgtg agcaggcgct
      601 cgacgcggcg gaggcgctgg ctgcggcggc cggtctccag atcgaattcg aggtcgcagt
      661 aggcgtcgag gagcggcggc agggacgcgg ccacctcgcg ggggagcggc gcccggccca
      721 ggttcgtgtg gaggaacagg ccggtggcat tgagcacccg gcggaggggg cctcccaagg
      781 cggcctccac ctcggcggcg atgcggccgg gaagcccggc gacggtgctc tcccgctcgt
      841 tttcgggcgg cgaagcggcg gtccagcgct cccggaggcc gtcgaccacg cgccgcgctg
      901 tgatgcggac ggggtctcgt ccgtataatg aaatgagggc tcgaagcgcc ggatcgccga
      961 gcagccggtc gatggcaggg atgccgcggc gggcatcccc gggaaaatcg ggggaatcgg
     1021 gcgaatcgga ggagtcgggg gagatcatcg cggcttcgag cattctagga gaatcgtgga
     1081 ccccaagcga accccgaggc cgggagccgg ccctgcaccc accctgaagc ccggaccggc
     1141 caaacccggc gcggcccccg gcgtgccgaa gcgtcccggg gcgggaccgg cgacgccaca
     1201 gccgccgggg cggcggggga ccaccagcta caaagagccc ctggaccggc tggcgaccga
     1261 catccggcag ctccagatcg aatttgagcg cttcttcgcc ggcgcgctgc cgatcccgcc
     1321 ggaggatctc cgcaaccgcg tccaggcgca gctcaggacc cttcgcaacg ccaccatcac
     1381 cgcggccgtg gacagcttcc ggctgggaga cctggaggcg cgcttcaaca cctacaacga
     1441 gctgttcaac cgccggctgc gcgagcggga ggaggggcgt caccgggcac ctcgcccggc
     1501 gccggtggag gttcagcgct tcgatccgct caagggcgtc gtggtgggcg cggacgtgga
     1561 ccccgaggcg gcggaggccc tgtaccaggg tctcgcctcg cgcccggcgt cgacgccgcg
     1621 cttcgacctc gacgccttcc ggacctatct ctcccgccag gtgtccgccc tgcaggccaa
     1681 gaccggctgc gccgaggtgc agttccggct ggccgaggag gagggcacgc tcaagctcaa
     1741 ggcccgtccg gtgaaccgcc agggctcgta gctctcgcga accaaggaga tgacattgcg
     1801 cagcaggacc attcgactca ccgcattctc actcgccgcc gtcctggtgg cggttctcgc
     1861 cctgtcggcc atcgcggccg ccccggcacc gccgaacatc ggcaaggccc tggaggagca
     1921 gcggcgcctc gccgccgagc gtccccagga cccggccgtc ttcaacgaca tgggcaacct
     1981 cctgatgctg gcccatcgtc cggccgacgc cgagacggcc tatcgccgcg ccgtcgagct
     2041 cgatccggcc aaggtctcgg cactcttcaa cctcggcctg ctgctccagc agcgggggga
     2101 ggaccgggag gcgctgagcc tgttcgagcg ggtggtgaag caggacgagc ggtacgcctg
     2161 ggcccactac cagatcggca ccatccggga gagccggggc caggaggcgg gagccatcga
     2221 cgcgtacgcc gcggccttct ccctggatcc gcagctcgcc ttctcggacg tcaatccgca
     2281 catcgtggag aaccgtctgg tgacccaggc gatgctccgc gcctacaagg ccgaccgaga
     2341 gctgccgcac gcgcccaagt tctatgacga cccgaaccgg atcgcggccc tgctggtgcc
     2401 gccgccggca ccccggaccc cgacgtccga tgaggagggc acgacccagc aggcggcgca
     2461 ggcccagccc ggtgcgcaga cgcgtcccgg tggccaggag ctgaaccgga acgacctcca
     2521 gccgggccgc cggatgggtc agccgcagcc gccgggcatg ggcggccgcc cggcgcccgg
     2581 tggcagcgtc cgtggtggct acgtcccgcc gggcggctat accccgcccg acccgaacgt
     2641 gccgcgtggc ctgcggcagt ggaaccgtcc ggagccgcag acggaggtga atcccggcga
     2701 aggcatgcag ccgggtcagg tgatcacccc gccgccgggc ggcgtctact accagcccgg
     2761 cctgccgagc accggccgcc tcgacatccg cctcctcccg ggctccaagc cgcgcgaccg
     2821 cgaagcgcgg agctgagagc cccttccctg cccgggggcg gagagactcc gcctccgggt
     2881 ttctcagttc ttccagaaca atctcaaaat agcaatttgg aagaagactt cgtctgcggc
     2941 tccggtcatg attcggctgc tgccagtctc tggccttctc cgtccggccg gacgggtttt
     3001 ctggatgccg tcctggggga ggaatgggcc tggcgcattt accacaggag gctgaagatg
     3061 aaagctttga acctgcgcaa gtcgtatctc gttgggctgg cgatcctcgc cctggccgtc
     3121 accgcgatct tcattaacac gtccggcctt tctgcccagt catcggaaga cgaataccac
     3181 acccttctgg gctatcacgg gtaccaaagc ccgggtggag tgatcttcga gtcggcctcg
     3241 acggagtcga ttagcgccaa cgtcgaggaa aagtatctag accgcaaggg cctggcgacc
     3301 cgggagctca acgtccgcgg catggcgggc aggggctatt ccgaggggct cggcgagacg
     3361 atgtactggc tggacaagag ccgtccggtg aaggggagcg tggtgagggc gaagactcct
     3421 ggggcggagt accctgcggt tcatgagatg catttccatc tgctcctcgt gtccgatatt
     3481 ttcccaggca agactctgcg gtcgatcaac ccggcgatca tggtgaacaa caacgccacc
     3541 tctttcccgc cgaagccggg tgcccggtat cagctcaaga acgtggtgga gcttgaggac
     3601 gtcgagaatc caggcgaagt catgttccgc atcgtgagca acaggaacca gattatcggt
     3661 gccgggcgtc tgccgctgga cccggcgatg gagccccagc gctgatctga acggcgaggc
     3721 cgggcgtgcc ggcgcccggc cgttctcgtg ggtggcggct tcctcccccc ctgtctgaag
     3781 attagcaatc tggaagaagt ctcgcggccg ggctccgtcc tacgatggcg gacgtacccg
     3841 agagcccgcg ggcctctcat tcaggagacg gaggattcca tgatcagaag cacgcgtctc
     3901 attgttgcca tcagcgccct cgccctggct ctggcagtcg tgccggccca ggcgttggac
     3961 aatgtcatct acaacggcag cgatctgtgg atgaccccgg ccgacggctc gacgtacgcc
     4021 gagttcaaga gcaatccgat ccctgccggt ttcttctgca acaagtccga gccgttcacc
     4081 ggccggatcg ccttcaaggg cgcgccgctg gcgaccgcgg acggctccct gggcaacacc
     4141 gacacgatcg tgcagcggct ggacgacgcg gtgttcaaca agcggggtat cgcctcgacc
     4201 cggattcagg tgcgggcgct gcagttcgag agcctggcgc cggtcaaaac ggcctgcggc
     4261 agcttccgcg tattcgtgcg cttgcagggt gagcagccgg tcacccggat gaagatcgtc
     4321 cgcgaaagcg aggacggcgg ccgcttcttc tcgccgatcg ccgtcaaggt gcggatgtcg
     4381 ttcgtgccgg tcgacggcaa gggccgtgag cgtctggagg tcacccgcag cctgcgcttc
     4441 aagccgagcg accacgcgtt ctggtcggtg ccgtcggagc aggcgctgga gaagcggggc
     4501 gtggcgctgg tcgacaccga cagcgacaac cgtccggaca cctacgtgcc gggcacctcg
     4561 aacttcgccg ccggccggcg tgcttcccag aacaaggcta tccagcagga cgactcggtg
     4621 ggcatcctcc acgacggcta cgagcactac cactacacca ccgccgccga atccaccacc
     4681 ggacccaccg ccggagccaa ctgacgcact ctccctttgg gccggcgccg cattccgatg
     4741 cggtgccggc tcggtcgcga tctttcaaca gttagcaatc tggaagaagt attggatcgg
     4801 tctttcctcc tatcctggcg tgtagacccg agagcccctc gcgactctca ttcaggagac
     4861 ggaggattcc atgctcagaa agacgcgtct gatggtgtcc atggccgccc tcgccctcgt
     4921 tctggcggtg gtgccggccc aggcgttgga caacgtcatc tacaacggca gcgatctgtg
     4981 gatgaccccg gccgacggct cgacgtatgc cgagttcaag agcaatccaa ttccggcggg
     5041 cttcttctgc aacaagtccg agccgttcac cggccggatc gccttcaagg gcgcgccgct
     5101 ggccacggcg gaccgctccc tgggcaacac cgacacgatc gtgcagcgga tggacgacgc
     5161 ggtgttcaac aagcgtggcg tcgcctcgac ccgcatccag gtgcgggcgc tgcagttcga
     5221 gagcctcgct ccggtcaaga cggcctgcgg cagcttccgc gtgttcgtgc ggctgcaggg
     5281 cgagcagccg gtcacccgga tgaagatcgt ccgcgagagc gagaacggcg gccgcttctt
     5341 ctcgccgatc gcggtcaacg tccggatgtc gttcgtgccg gtcaacggca agggccgtga
     5401 gcgtctggaa gtcacccgca gcctgcgctt caagccaagc aaccatgcgt tctgggcggt
     5461 gccgacggag caggcgatcg agaagcgggg cgtcgccctg gtcgacaccg acagcgacgg
     5521 ccgtccggac acctacgtgc cgggtacctc gaacttcgcc gccggctggc gtgcttccca
     5581 gaacaaggag atcttgagct ccgaggcgga gcgctccgcg gtcctccatg acggctacga
     5641 gcactaccac tacacctacc cctccgagac cgccgacacc cagatctaca attgagctcc
     5701 catcacccgg aggcggagag ccttcgcctc cgggtccttt ctccccgggc gggctccgtt
     5761 cccggggttt cccttcggca gtcttgggcc gccggcaaac aaagtcgtgt cacggcattt
     5821 gccggcgcct catggcgttt gaagtcgtcc cactggaaac gaagtcggct cccggcgttt
     5881 gaagtcgacc ggcggcgttt ggagcgtccc gccgcgtctt cagtcgagcc acggcgttta
     5941 ccggcgtctc acggcgtttg aagtcgtccc attggaaacg aagtcggctc acggcgtttg
     6001 aagtcggcct gcggcgtttg gaggcgtctc gccgcatctt cagtcgagcc acggcgtttg
     6061 ccggcggctc gcgggaggtg aaggaggggg gtagctttta gggcggaggg aggcgccacc
     6121 ggcagactcc ttactcgccg gtggcaccct actgcaggga atgtcctact cgcggttcgc
     6181 cgccatgtcc gccgccaccc gttcatcgag gatggcgccg caccgctcga gcgccaccgc
     6241 cccggccgcc tccagcacga aagccagggc ctcgggcgac cgcagcagat gcaccagcac
     6301 gttcatggcg tcgacggctc tctcctcgaa cagcgggaag aacccgaccg gcccgccgtc
     6361 ctcgtcatcg ccaccgccgt ccccgctgcc acccccggcg agagccaagg gataggcgcc
     6421 gtgagcgtcg ggctgcttgg agaggagaag gagcggatcc cgacccgtgc cgggaaggat
     6481 ggccgcgcag acgagcgcga gccagcgaga ggggaagacc gccgccgggg cgaagccgcg
     6541 ggccaggctc tcgcccaccc ggaacaggcc gaagccatgt ccaggaatgg gctcgatgcg
     6601 ccagggcccg gccacgtcgg catcgccggc cgtgggcggc tcgtcccgct cagccagacg
     6661 gctgaggaag gcgacagtaa acgcgttggg atgatggggc ggattgggta cggtggtacc
     6721 cttcgggtag ccatgcatat gtcctccaca cggactgtgt agggttaggc ctgggagcga
     6781 gggtccaagc tcgcttccgg gccgcaagcc ggcaccatgc cggccgaccc tccttcaaga
     6841 gatgttgtcg ggatcttcct gacttcgctt ccgcttctcc tcgataccct ccctcctccc
     6901 ggaagactcc acgccgggtt tctgtatgac tccgactata gcgagggagg ggagggcggt
     6961 caagaggtga gacggagatg ttctgctctg gagataggta ggaggtggtg cacctgaggg
     7021 atgacctcct tgccgtttga cacctcatgg ttgctggcct accctgtgct aaaggtttgg
     7081 acgtagaaag ctttacttcg tgtgccccac aatcgagtgc gggttcaggt tgggtgccct
     7141 ggaggaagca tgcagcgaat tgcgggaatt gacgggtgcc agtcacgttg gcttgcgatc
     7201 acccatgagc ccggcactcg atcgttcgtg ccgcaggtgc tcgagaccga ggagcttcca
     7261 ggtcaaccat gggcgctggc tgctattgac atcccaatcg ggttgcctga ccgtggctcc
     7321 cgagaggccg atcgcgcggc ccgtaaattc gtgggacccc gcgccagcag cgtgtttccg
     7381 tgcccaatcc gtccagcgct tgacggactt tcctgggaag cagcatgcga gatcacatac
     7441 cagatcaacg gccatcgtat ctcacggcaa acgtttgcaa ttctgtcgaa gattcgggcc
     7501 gtcgatgagt gcatacggtc aaccaacttg cagaaacgcc tctttgaggt ccatcccgag
     7561 gtctcgttcg cctcttggaa cggcgctccg acgcttcttc cgaagaagga tgcccgcgga
     7621 catgagcagc gccgaactct catcgcaagg cacttcgggc cagaggcgtt cgccagtgtt
     7681 gtcgcacaga tcggcgagcg tagtgtcgcc gcagacgata tcgccgacgc ctttgccgca
     7741 ctctggtccg cggagcgaat tctgaacggc accgctcaac gactgccgag tgactcagtt
     7801 agcgactctt atagcctccc aatgcacatc tggtattagg cctctccctt gcctgagcag
     7861 gttctgcggt gagcgaggtc agctcacgga tgtcgagcgg cgggaactgc tccggtatct
     7921 ggtcgcttac ctggagggta ggcgggtcta accgggcccg gcgatgctct ggtctgctac
     7981 catgcaggag ggcttccggc gccgtatcga gcctaggttg gccgggaccc cgaagggagc
     8041 ttgcagacca tgaccaccac cttggcctat cttgaacgtc tctcgcgtga gatccataag
     8101 ccggaagccg agatcatgac gatggccttc gaggccggcc tccgccaact ctggcgcgag
     8161 cacatcctcg gacagtatct gcggggtgag attaccaggg agaaggctat cgagagcgtg
     8221 gggatcgact gggtggattt ggcggagcgc cagcgcgtgg cgacgaacga ggatctggat
     8281 tgggctctga acacttgatt gcggtcgccg atgccggtcc cctgatccac ctcgcagaaa
     8341 ttgactgcct atccctcctc gcagtttttc cagagctcca tgtaccggag ggcgtgtggc
     8401 ttgaggtgga aaggccttcg tcaatccgga ccgagttgac cttcgcgaag cggcatgttc
     8461 tcgcgcagga cgaggttcaa aagttcaccg cggaacacgg gctggggaag cttcaggcag
     8521 gagaacggga aagcctattg ctctgtgtga ggatctcggc ttcggtgctg ctgaccgacg
     8581 atctggcagt acggcgggct gccaaggatc tcgcgctgac gcctgttggc tctctgggtg
     8641 tcatcgcgag agcccaccgg atgggccgga tctccgctga ggttgccgag cgcctcctcc
     8701 gtcagttgga tacagcgagc agcctctacg tcacgccggc tatcgtggac ctggcaatcg
     8761 agcgcctacg ggatgagggt tcgagtaaat cttgacctga ccccctacac attgaacctg
     8821 aagtgagcca catccccatc ctgcatcacg tactccttcc cctccagccg cagcagcccc
     8881 gcgtccttgc aggcggcgag ggtcttgagg cggatgaggt cgtcgcagtg gacgacttcg
     8941 gcgcggatga agccgcgctc gatgtcggag tggatggcgc cggcggcttc acgggctttg
     9001 ttgccgcggc ggatggtcca ggcccgcact tcgtcctctc caacggtgaa gaaggagatc
     9061 acgccgagga gctcgtagct ggcgcggttc acgcggtcga ggctcggctc ggtcaggccg
     9121 aggtcggaga ggaagtcctt ctgctcttcg ggggagagct ggccgatctc ctgctcgatg
     9181 ggggcgc
//
LOCUS       DASPJI010000449         6795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_491795, whole genome shotgun sequence.
ACCESSION   DASPJI010000449 DASPJI010000000
VERSION     DASPJI010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491795"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VEL74_12175"
     CDS             <1..532
                     /locus_tag="VEL74_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63332.1"
                     /translation="VSANDNDKFQYNARVTFQPFGDVKYSEGDFESSDRPLFAIAGQY
                     ESNDRQGATTSNDIDKEIVGGDVVFKYKGLFAFAEYFAGTNDPETGIDVDDEGWNAQL
                     GYFVIPQKLEIAGRYAVLDRNADRDDDEQEERGIALGYFFNKHPHKLQLDYRQLENKA
                     TDATDDELRLQYQIIF"
     gene            complement(621..1280)
                     /gene="phoU"
                     /locus_tag="VEL74_12180"
     CDS             complement(621..1280)
                     /gene="phoU"
                     /locus_tag="VEL74_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HYG63333.1"
                     /translation="MERRLDQDLDRVRQSLLRMGGMVEGMVAQATQALLERDSVLCKK
                     VIDGDRDVDQLEIEIDEICHSVLGRKQPTAVDLRFLVAVMKINSDLERIGDSAVNIAQ
                     SVEQLNEQPPLKPYIDLPHMSQLVQTMVRKSLDAFVRRDAALATEVCRSDDAVDGLYK
                     QIFRELLTYMIEDPKTVSRALHLLLISRNMERIADHATNIAEDVIYYVEGRDIRHSMA
                     G"
     gene            complement(1368..1745)
                     /locus_tag="VEL74_12185"
     CDS             complement(1368..1745)
                     /locus_tag="VEL74_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HYG63334.1"
                     /translation="MKTIAVLGASKDRSKFGNKCVRAYRQPGYEVFPVNLGSSDPIEG
                     LTTYRSLADVPGELERISVYLPPPVTMELLGDIAAKGAQEVWFNPGSADSQVLEEARR
                     RGIKAVPGCSIIDVGMSPAQFPG"
     gene            complement(1758..3689)
                     /gene="selB"
                     /locus_tag="VEL74_12190"
     CDS             complement(1758..3689)
                     /gene="selB"
                     /locus_tag="VEL74_12190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HYG63335.1"
                     /translation="MRRVIVGTAGHIDHGKTSLVRALTGIDCDRWAEEKARGITIDIG
                     FAHLVRDDLQVGFVDVPGHERFLHNALAGLGGIRLMLLVVAADEGVKPQTREHLAVCS
                     LLGIPAGLVALTKADLVAPDLLELAQLEVEDLLAGTPFAGAPILPVSSTTGQGIPELE
                     ATLVDLAGLWATPPDPERPARLPIDRAFHLKGLGAIVTGTLASGAIRAGDTLDVLPPR
                     PGSEGRARVRSIQVHGEAREEAEAGERTSLQLTGISLEELERGLHLTTPGAFAAAPSL
                     LARFTLLADAPAPLRGFLPVRLHLYASEVVGRMRPLAEGGLAPGETGPVEIRLEAPVV
                     AVRGDRYVIRRPSPAATLGGGEILDPQWRRHRGAVLRPALAALQGDLRAALAFWVQEA
                     GERGVEAPEIARRLGTRPEKVAAELSALAKAKGLLLEVPEGHGHGRRWIAAGAVERVS
                     ERARRVLKEHFQKDRLAEAMPKAEAVRRILRGRAAELAHVYFGWLEAQKVLAVQGEKV
                     TLPGRSADLTGEESKLSAEVLAAFDKGGLTPPSPSELQQALSAKPQILEGVVRHLVSR
                     GRLVKLPSGLILAASAVQELRRQLLSTSWDQFSVADFKDRFGLSRKWAIPLLEHLDST
                     GATRRMGDNRMVVRPGPPT"
     gene            complement(3689..4144)
                     /locus_tag="VEL74_12195"
     CDS             complement(3689..4144)
                     /locus_tag="VEL74_12195"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HYG63336.1"
                     /translation="MDTTPQQRIEADVKAAMKSGEKEKLSTLRMLLTEIKNERIRKGS
                     DVDEAGFVALVRKTIKQREESATQYRKGNREDLAVKEDSEKEFLATLLPAQVDEGQIR
                     AAIEALVAERGLSGPAAIGVVMKETLARFGSSADGSTINRIAREVLAKG"
     gene            4208..4579
                     /locus_tag="VEL74_12200"
     CDS             4208..4579
                     /locus_tag="VEL74_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HYG63337.1"
                     /translation="MERDTQQRRALWSAIEGAGRPLAPRELLELAQASCPGLGIATVY
                     RALKAWTEDGRLAQVDLPGEPPRWEPTGKGHHHHFHCRACDRVYELEGCPDGLRSMTP
                     EGFSLESHEVVLYGRCSECAG"
     gene            4576..5496
                     /locus_tag="VEL74_12205"
     CDS             4576..5496
                     /locus_tag="VEL74_12205"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HYG63338.1"
                     /translation="MTTGLLLSVLLLAGCRLAPVAPGAAAPARPSGGPLQVAVSVAPQ
                     AYFVERIGGRHVRVEVMIPPGYSHVDYPLTPWKVMALQRAALYIEVGHPGFEFESRFI
                     DPYLAGRPDVRVVDMSEGMDLIEEGHGEGSHPEDEHGHDHSSGDPHVWVSPGSVRVAA
                     GNIAAALIELDPAHAADYRANLRRFLADIDRLDREIRARLAGREGAAFMVYHPDWGYF
                     AREYGLRQVAIESGGKEPSARRLIRLIEEARADGVKVIFVQGGFPRKSAQVIADAVGA
                     RVVTADPQREEWLDNLREVSVALEEALPRA"
     gene            5489..6271
                     /locus_tag="VEL74_12210"
     CDS             5489..6271
                     /locus_tag="VEL74_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HYG63339.1"
                     /translation="MPETSSSPLISVRDVTFGYGREVVLDRVSLEIHSHDFLALIGPN
                     GGGKTTLIKVMLGLLAPWSGEVVYHLPERHAGPRKGRLGYVPQFSTFDRNFPLRVSEM
                     VLMGRAGGRSLVRPYSREDRERVGHELERLGLTGIAGSHVGEVSGGQLQRALIARALV
                     ADPEVLFLDEPTASIDAESRETLRGLLEELNQRIPVVVVTHDVSSISPQVRTIACINR
                     KLHYHGDAEIGPDVLQEVYGEPVELVTHGVPRRVLHDHHHHH"
     gene            6284..>6795
                     /locus_tag="VEL74_12215"
     CDS             6284..>6795
                     /locus_tag="VEL74_12215"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HYG63340.1"
                     /translation="MPTLSAALSYDFLQNAVLAGVLAAVLCGVIGTFVVIKRLAFISD
                     GISHAAFGGMGVCFFLGVDPVLGAVPVALACAVALGAVDTETIRSYDSIIGVLWAVGL
                     AVGIVFVYKTPGYAPNLMNYLFGNILMVSHRDVVTILGLAASVLLILLLFWKGLVAVA
                     FDEVFARVQGL"
BASE COUNT          966 a         2365 c         2395 g         1069 t
ORIGIN      
        1 ggtgagcgcc aacgacaacg acaagttcca gtacaacgcc cgtgtgacct tccagccctt
       61 cggcgacgtc aagtactccg agggggactt cgagagctcc gaccggccgc tcttcgccat
      121 cgccgggcag tacgagtcca acgaccgcca gggcgccacc accagcaacg atatcgacaa
      181 ggagatcgtg ggcggcgacg tggtgttcaa gtacaagggg ctgttcgcct tcgccgagta
      241 cttcgccggc accaacgacc ccgagaccgg gatcgatgtc gacgacgagg ggtggaatgc
      301 ccagctcggc tacttcgtga tcccgcagaa gctcgagatc gccggccgct acgccgtcct
      361 cgaccgcaac gccgaccgtg acgacgacga gcaggaggag cgggggatcg ccctcggcta
      421 cttcttcaac aagcaccccc acaagctgca gctcgactac cgtcagctcg agaacaaggc
      481 gacggacgcc accgacgacg agctccgcct ccagtaccag atcatcttct aggctagagt
      541 gggaatgggc cgggggccgc gcttcggcgc ggcccccttt ttacttccgg tttcagcgag
      601 cggatgggga agggaagggg tcagcccgcc atggagtggc ggatgtcgcg gccttcgacg
      661 tagtagatga cgtcctccgc gatgttggtg gcgtggtccg cgatgcgctc catgttgcgc
      721 gagatgagca gcaggtggag cgcgcggctg accgtcttgg ggtcttcgat catgtaggtc
      781 agcagctcac ggaagatctg cttgtaaagg ccgtccaccg cgtcgtcgga gcggcagacc
      841 tcggtggcca gggcggcgtc gcggcgcacg aaggcgtcga ggctcttgcg gaccatcgtt
      901 tgcacgagct gcgacatgtg gggcagatcg atgtagggct tgagcggcgg ctgctcgttg
      961 agctgctcca ccgactgggc gatgttgacc gccgaatcgc cgatccgctc gaggtcgctg
     1021 ttgatcttca tcaccgccac cagaaagcgc agatcgacag ccgtcggctg tttgcgtccc
     1081 aggacgctgt ggcagatctc gtcgatctcg atttccaact gatcgacgtc gcggtcgcca
     1141 tcgatcactt tcttgcagag cacggaatcg cgctcgagca gcgcctgcgt cgcctgcgcg
     1201 accatcccct cgaccatgcc gcccatcctg agcaggctct gccgtacgcg atcgaggtct
     1261 tgatccagcc gtcgttccat gatggtgttc ctttcgtccc gccggatctt aacccgcgac
     1321 agtcacaaga tcgggacgag gccgtgacag tcgtgtggcg gcggtcctca ccccgggaac
     1381 tgggccgggc tcatccccac gtcgatgatg ctgcaccccg gaacggcctt gatcccccgg
     1441 cggcgggcct cctccagcac ctgggagtcg gcggagccgg ggttgaacca gacctcctgc
     1501 gcccccttgg cggcgatgtc gcccagcagc tccatcgtga ccggcggcgg caggtagacg
     1561 cttatccgct cgagctcgcc cggcacgtcg gcgagactgc gataggtggt gagcccctcg
     1621 atcgggtccg agctgccgag gttcaccggg aacacctcgt agcccggctg ccggtaggcg
     1681 cggacgcact tgttgccgaa cttgctgcgg tctttggagg ctcccaacac ggcgatggtc
     1741 ttcatgatgt ctccttctca ggtaggtgga ccggggcgta ccaccatgcg gttgtcgccc
     1801 atgcgccggg tggcgccggt ggagtccaga tgctcgagca gcgggatcgc ccacttacgg
     1861 ctgagcccga agcggtcctt gaagtcggcg acggagaact ggtcccagga ggtggagagg
     1921 agctggcggc gcagctcctg cacggccgag gcggcgagga tcaggccgct gggcagcttg
     1981 accagccggc cccgcgacac caggtggcgg acgaccccct ccaggatctg cggcttggcg
     2041 gagagggcct gctgcagctc cgaggggctg ggcggggtga gccccccctt gtcgaaggcg
     2101 gcgaggacct cggccgagag cttggactcc tcgcccgtga ggtcggcact gcgcccgggg
     2161 agcgtcacct tctccccctg gacggcgagg accttctgcg cctccagcca gccgaaatag
     2221 acgtgcgcca gctcggcggc ccggccgcgg aggatgcggc gcaccgcctc ggccttgggc
     2281 atggcctcgg cgaggcggtc cttctggaag tgctccttga gcacccgccg ggcgcgctcg
     2341 gagacgcgct ccaccgcgcc ggccgcgatc cagcggcggc catggccatg gccctcggga
     2401 acctccagca gcagcccctt ggccttggcc agggcggaca gctccgccgc caccttctcg
     2461 ggccgcgtgc ccaggcgccg ggcgatctcc ggcgcctcga cgccgcgctc gccggcctcc
     2521 tgcacccaga acgcgagggc ggcgcggagg tccccctgga gggcggcgag ggccggccgc
     2581 agcaccgcgc cgcgatggcg ccgccactgc gggtcgagga tctcgccgcc gccgagcgtc
     2641 gcggcggggg aggggcggcg gatcacgtag cggtcgccgc gcacggccac cactggcgcc
     2701 tcgagacgga tttccaccgg ccccgtctcc cccggcgcga gccccccttc ggcgagcggg
     2761 cgcatgcgcc ccaccacctc gctggcgtag aggtggaggc gcaccggcag gaagccgcgc
     2821 agcggcgccg gggcgtccgc cagcagggtg aagcgggcga gcaggctggg cgccgccgcg
     2881 aaggctcccg gcgtggtgag atggaggccg cgctccagct cctccagcga gatgccggtg
     2941 agctggaggg aggtgcgctc gccggcctcc gcctcctccc gcgcctcgcc atgcacctgg
     3001 atgctccgca cccgcgcccg cccttcgctc cccggccgcg gcggcagcac gtccagcgtg
     3061 tcgcccgcgc ggatggcgcc gctggcgagc gtcccggtga cgatggcgcc caatcccttc
     3121 agatggaacg cccggtcgat gggcaggcgc gccggccgct ccgggtccgg cggcgtggcc
     3181 cagaggccgg cgagatcgac cagcgtggcc tcgagctcgg ggatcccctg gccggtggtg
     3241 ctggagaccg gcaggatcgg cgcgccggcg aacggggtgc cggcgaggag gtcctcgacc
     3301 tcgagctggg cgagctccag caggtcgggc gccaccaggt cggccttggt gagggcgacg
     3361 agcccggcgg ggatgccgag gagcgagcag acggccaggt gctcgcgcgt ctgcggcttc
     3421 accccctcgt ccgccgccac caccagcagc atgaggcgga tgccgcccag gccggcgagc
     3481 gcgttgtgga ggaagcgctc gtggccggga acgtcgacga agccgacctg gaggtcgtcc
     3541 cggaccaggt gggcgaagcc gatatcgatg gtgatgccgc gggccttctc ctcggcccag
     3601 cggtcgcagt cgatgccggt gagggcgcgg acgaggctgg tcttgccgtg gtcgatgtga
     3661 ccggcggtgc cgacgatgac gcgccgcatc agcccttcgc gaggacctcg cgggcgatgc
     3721 ggttgatggt ggagccgtcg gccgacgagc cgaagcgggc gagcgtctcc ttcatcacca
     3781 cgccgatcgc ggccggtccc gaaagccccc gctcggcgac gagcgcctcg atggcggcgc
     3841 ggatctgccc ctcgtccacc tgggcgggga ggagggtggc gaggaactcc ttctcggagt
     3901 cctccttgac cgccaggtcc tcccggttcc ccttgcggta ctgggtcgcc gactcctcgc
     3961 gctgcttgat ggtcttgcgg accagggcga cgaagcccgc ctcgtccacg tcgctgccct
     4021 tgcggatgcg ctcgttcttg atctcggtca ggagcatccg cagggtggac agcttctcct
     4081 tctccccgga cttcatggcg gccttgacgt cggcctcgat gcgttgctgc ggtgtcgtgt
     4141 ccatgcggcg gattatagga gagggggtgg aataccctga tcctgagaat gcattatcat
     4201 ccttgccatg gaacgggaca cccaacagcg ccgcgccctg tggagcgcca tcgagggggc
     4261 gggacggcct ctcgcgccgc gggagctgct ggagctggca caggcgtcct gcccggggct
     4321 ggggatcgcc acggtctacc gcgcgctcaa ggcgtggacg gaggacgggc ggctggcgca
     4381 ggtcgacctc cccggcgagc cgccgcgctg ggagcccacc ggcaaggggc atcatcatca
     4441 cttccattgc cgggcctgcg accgggtcta cgagctggag ggatgccccg acggtctccg
     4501 cagcatgacg ccggagggct tctccctgga gtctcacgag gtggtgctct acgggaggtg
     4561 ctccgagtgc gcgggatgac cacgggtctg ctcctctccg tccttctcct cgccggctgc
     4621 cgcctggctc cggtcgctcc gggcgccgcc gcgccggccc gtccgtccgg cggccccctc
     4681 caggtggcgg tgagcgtcgc tccgcaggcc tacttcgtcg agcggatcgg cggccggcac
     4741 gtgcgggtgg aggtgatgat ccctcccggc tactcccacg tggactaccc gctgaccccc
     4801 tggaaggtga tggccctcca gcgcgccgcc ctctatatcg aggtagggca ccccggcttc
     4861 gagttcgaga gccgcttcat cgatccctac ctggccggca ggccggacgt ccgggtggtg
     4921 gacatgtccg agggcatgga cctcatcgag gagggacatg gggaaggaag ccatcccgaa
     4981 gacgagcacg gccacgatca ttccagcggc gatcctcacg tctgggtgtc gcccgggagc
     5041 gtgcgggtgg cggccggcaa catcgccgcc gcgctgatcg agctcgaccc ggcgcacgcc
     5101 gccgactacc gggcgaacct gcggcgcttc ctcgccgaca tcgaccgcct cgaccgggag
     5161 atccgcgcgc ggctcgccgg ccgcgagggt gcggcgttca tggtctacca cccggactgg
     5221 gggtacttcg cccgcgagta cggcctgcgc caggtcgcca tcgagagcgg cggcaaggag
     5281 cccagcgccc ggcggctgat ccggctcatc gaggaggcgc gggcggacgg ggtgaaggtg
     5341 atcttcgtcc aggggggctt cccccgcaag agcgcccagg tgatcgccga cgccgtcggc
     5401 gcccgggtgg tcaccgccga tccgcaacgc gaggagtggc tcgacaacct gcgtgaggtg
     5461 tccgtcgcgc tggaggaggc gttgccccgt gcctgagacg tccagctccc cgctgatctc
     5521 cgtccgcgac gtcaccttcg gctacggccg cgaggtggtg ctggaccgcg tgagcctgga
     5581 gatccattcc cacgacttcc tggccctcat aggcccgaac ggcggcggca agacgacgct
     5641 gatcaaggtg atgctggggc tgctcgcgcc ctggtccggc gaggtggtct accacctgcc
     5701 cgagcgtcat gcaggaccac gcaaggggag gctgggctac gtgccgcagt tctcgacctt
     5761 cgaccgcaac ttcccgctgc gcgtctcgga gatggtgctg atgggcaggg ccggcggccg
     5821 tagcctcgtc cgcccctact cgcgggagga ccgggagcgc gtcggtcacg agctggagcg
     5881 cctcggcctc accggcatcg ccgggtcgca cgtcggcgag gtctccggcg gccagctcca
     5941 gcgggcgctc atcgcccgcg ccctggtggc cgacccggag gtcctcttcc tggacgagcc
     6001 gaccgcctcc atcgacgccg agtcccggga gaccctgcgc ggcctcctgg aggagctcaa
     6061 ccagcgcatc cccgtggtgg tggtcaccca cgacgtcagc tccatctcgc cgcaggtgcg
     6121 gacgatcgcc tgcatcaacc gcaagctcca ctatcacggc gacgccgaga tcggccccga
     6181 cgtgctgcag gaggtctacg gcgagccggt ggagctggtg acccatggcg tgccgcgccg
     6241 cgtgctccac gaccatcacc accaccacta gacgaggctc ctgttgccca ccctctccgc
     6301 cgcgctgtcg tacgacttcc tgcagaacgc cgtcctcgcc ggagtgctgg ccgccgtgct
     6361 gtgcggcgtc atcggcacct tcgtggtcat caagcggctg gccttcatca gcgacggcat
     6421 cagccacgcg gccttcggcg gcatgggagt ctgcttcttc ctgggggtcg acccggtgct
     6481 cggcgccgtg ccggtggccc tggcctgcgc cgtcgccctg ggagcggtcg atacggagac
     6541 catccgctcg tacgactcga tcatcggcgt cctctgggcg gtgggcctgg ccgtgggcat
     6601 cgtcttcgtc tacaagacgc cgggctacgc gccgaacctg atgaattacc tgttcggcaa
     6661 catcctcatg gtgtcgcacc gggacgtggt gaccatcctg gggctggctg cttcggtgct
     6721 gctgatcctg ctgctcttct ggaaggggct ggtcgcggtg gccttcgacg aggtcttcgc
     6781 ccgggttcag ggcct
//
LOCUS       DASPJI010000450        10142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_661031, whole genome shotgun sequence.
ACCESSION   DASPJI010000450 DASPJI010000000
VERSION     DASPJI010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10142)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10142
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661031"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..6764
                     /locus_tag="VEL74_12220"
                     /pseudo
     CDS             60..6764
                     /locus_tag="VEL74_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
     gene            7509..>10142
                     /locus_tag="VEL74_12225"
     CDS             7509..>10142
                     /locus_tag="VEL74_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG63341.1"
                     /translation="MVPGAFVFLEALPITAHGKVDRAALPDLEVERVGVEFVAPRTPL
                     EARIVEACAEVLGLEAVGVGDNFFELGGHSLLATQLVSRLRERWGIELPVRAVFEART
                     LGDLAERVTGWEPAGRIPRRPEGSGPAPLSFAQERLWFLDRIEAGTAYSMPMALALRG
                     ELSVPAMEAAFGELVRRHENLRTTFHDLQEGAVQVVEPAGDWRLLLVDLTGVAEGREA
                     EVGHLLATGAWRRFDLENGPLLRAALLRKGATEHLLILDMHHIVSDGWSMGVLIREVS
                     ALYAAALAGRPSLLPELPIQYADFAVWQRAWLEAGELERQLGYWRERLAGAPALDLPT
                     DRPRPAVQSFRGATLRFEIAPEETRGLETLARQRDASLYMVLLALSQTLLGRYTGQED
                     LVTGGAIANRNRSELEPLIGFFVNSLVLRADLAGDPPFSDLLDRTRRTTLEAYSHQDL
                     PFERLVEELQPERRLSHNPLFQVMCVLQNAPGGRIDLPGLRFEPMQLHFPTTRFDLEM
                     MFWEQGEGLGGHFTYATDLFEEATVQRMIGHLLNLASGAIETPGRRLSDLPLLAPAER
                     CQLLHEWNDTAAPLPDATLLDLFREQVAHRPDAIALSSPEGDLTYFALHHRATRLAHH
                     LATLGAGQEVPVALLADRSPSMIIGLLAILEAGGAYLPLDPSYPPDRLAHMRETAGAD
                     LLLANPALVPEELHQGTLIPLTHLGEGGTWESGPGAGDWTRWAGGEGGAPEGAEQGPL
                     PQVRGDNLAYVMFTSGSTGRPKATGVTHRNVVRLVRESRFADLGADQVFLQLAPISFD
                     ASTLEIWGALANGGRLVLAPAGRTALEDLASTIETGGVTTLWLTAGLFHQMVDGPIAS
                     LRGLRQLLAGGDVLSPDHVRRA"
BASE COUNT         1206 a         3615 c         3738 g         1583 t
ORIGIN      
        1 cgtcggcggc gccacgcact ccgatcgagg agatcgtcgc cggcctctgg gccgatctgc
       61 tggagacgag gccggccgga cggggcgacg acttctttgc cctcggcggc cattcgcttc
      121 tcgccgcgcg gctcgtggcc agggtgcgcg aggccttcgg tgtcaacctc cccctgcgcg
      181 ccgtcttcgc gcggccgacg ttggcggcct tcgccgccga gatcgagacg gctctcggcg
      241 ccggcacacc ctcggagccc ctggcgctcc tcccccggga cggcgcgccg ctgcccgcct
      301 ccttctccca ggagcagctc tggctgttcg accgcctcca gccggggagc gccgcctaca
      361 acctccccgt ggccttccgc atcctgacgg atggctcgaa gctggacgtg acagggctcg
      421 ccgccgcgct gacccgcctg gcggagcggc acgaggcgct ccgtaccacc ctggaggccg
      481 acgcccaggg acggatcttc cagcgtgtcc acgctcccgc ggcggccgtc ctccccctgg
      541 tggacctctc agggctgccc ggggaggcgg gctgcgccga ggcggcgtgt ctggtcgtgg
      601 ccgaggccgg tcggccgttc gacctggccg ccgggttgct cttccggacc accctcctgc
      661 gtctgtcttc tgaagagggg gctggcgagc accggctgct gctgaccctc caccacgcca
      721 tcgccgacgc gcgctccctg gagcttctct tcggtgagct cgtggagctg tacgagggcg
      781 cctccctgcc gccggtgccg gcgcagtacg ccgactttgc cgcctggcag cgcgctcgcc
      841 tgtcgggcga ggccctggag agccttctcg gcttctggcg ccggagcctc gcgggggcat
      901 cgccggtgct ggatctcccg gcggaccacc cgcggccggc gatccagagc ttccggggag
      961 cgatccgcca gacggctctc cccaatgtgg acccggagcg gcttcgcgcg ctgtgccggg
     1021 ccgagggggt cacccccttc atgctccacc tggcggcttt cgagaccctg gtccaccggc
     1081 ttaccgggct ggaggacttc gcgctcggca ccccggtctc catccgctcg cgagaggagc
     1141 tcgaaggggt ggtgggcttc ttcgtcaacg tcctcgtcct gcgcgccgag ccgaaggggg
     1201 acgcctcatt tcgcgagctg ctgggccgcg tccgtgccgc ggccctcgcc gcctatgccc
     1261 actccgagct gccgttcgag gcgctggtgg cggatctggc gccggagcgg agcgccagct
     1321 acacacccgt cgtccaggtg atgttcgcgc tggaggagga gccggacgcg cggaccggcg
     1381 gcgggctggt gtggaacccc caggggaccg atcgcggcgc cgcgcagttc gacctcagcc
     1441 tctcggtgcg gcagggggga gacgggctgc ggatcgcggc cgagtacgcc tccgccctgt
     1501 tcgacgcgcc gacggtagac cgcttcctgg gctggatggg gaccctcctc gccggggtcg
     1561 caaccgatcc cggccagccg ctgggcgacc tgcccctcct gggggaggcg gagcgtgccc
     1621 agatgctggt cgagtggaac gacaccgcgg agcggatcga gtgggaggct ccggtccacg
     1681 agctgttcgc gcgccaggcg gagcggacgc ccgacgcgat ggcggtggtg gtggcgggcg
     1741 agtccttcga ggggatgacc tatggcgagt tgcgccgccg ggccggccgc ctggcgcggc
     1801 ggctgcgcgc cctggggatc gagcccgagg tgccggtggc ggccttcgtc gagcgctcgc
     1861 tggacatggc ggtgggcctc ctcggcatcc tggagtccgg gggcgtgttc gtgcccctcg
     1921 acccgggcca tcccgccgac cggctggcgc tcgtgctgga ggactcggga gcaccgttgg
     1981 ccgtcaccca ggagaggctg ctctcccggt tgccggcggg cacggtgcgt ccggtgcttc
     2041 tccacgagag caacgtcgat gaggaagcgg cgcttccagc cccgccgctg ctgccggagc
     2101 atccggtcta tatgatctat acgtccggct ccacgggccg gcccaagggc gtggtgatca
     2161 gccagcgctc cttcgctcac cacaaccggg tcttcccggc gctgtgcggg ctccggtcca
     2221 cggatagggt gctgcagttc gcctccctct ccttcgacgt ggcggtcgag gagatgatcc
     2281 ccacctggct caccggcgcc tgtgtcgtcc tgtggcccga ggctgccgtg tccgggccgg
     2341 cggagctgac ccgtctcgtc gagcgcgagg agctgacctg cttccagctc tccacccagt
     2401 actggcagga ctagacgacg gagctggcgc gggcgcgccg gcccctgtcg caggcattgc
     2461 cggtcttgcc gtcgctgcgc tggctgatgg ccggcggcga ggcggtctcg gcggcgcatc
     2521 tggcggtctg gcgcgaaaca gtgggcgacg ccgtaacctg gttgaacggc tacggcccga
     2581 ccgaagcgac ggtgacctcc agcggctacc cgctgccgcg tggtggcgtg cagccgcgcc
     2641 agggctcgac ccactcggtg ccgatcggcc gtccgttcac caacgagcgg gtctacgtcg
     2701 tcgactacct gggccggccg gtgccgcccg gggcggcggg cgagctgtgg ctcggcggcc
     2761 ccggcctggc gcgcggctac gccggccgtt cggacctcac cgcgacccgt ttcgtgcccg
     2821 acccgttcgc cggcacccct ggcgaacggc tgtaccgcac cggcgacctg gtccgcttcc
     2881 tccccacggg cgatctggag ttcctgggcc ggctcgaccg gcaggtgaag ctccgtggtt
     2941 tccggatcga gctcggagag gtggagtcgg cgctcgccgc ctgcccgttg gtaaaggctg
     3001 gagcggtgct catcgagggg gaggggacgg cacggcggct ggtcggctat gtgtctccca
     3061 gcggtccaga ggtgacagga cgggagcttc gggcctggct gcgcggcatg ctgcccgagc
     3121 ccatggtgcc cggggcgttc gccctcctgc ccgagctgcc gatcaccgcg agtggcaaga
     3181 tcgaccggca ggccctgtcg cggcttaagg tcgaggcccc ggaagcggag gaggaggcgg
     3241 tggccccccg tacgccggtg gaggagatcc tcaccggcat ctgggccgag attctgagcc
     3301 tgccgcgggt tggtgtccac ggccacttct tcgagctcgg cggccattcg ctcctggcga
     3361 cgcgggtggt gtcgcgggtg cgggaggcgc tgggggtgga actgtcgctg gcggcgctgt
     3421 tcgagcggcc cactccggcc gctctggcgc ccctggtgga ggggctgagc ctggccgccg
     3481 ggtcgccgcc ggcgccgccc atcgtgcccg tcccgcgaga tggcgacctg ccgctctcct
     3541 ttgcccagga gcgcctctgg ttcatcgacc gcctccagcc tggcggctcg gcgtacaaca
     3601 tggtgaccgg cctggagttg gccggcgacc tctcggcggc gggcctcgag tttgccctgc
     3661 gcgaggtggt gcggcgtcac gagagcctgc gcaccacctt ccggcaggga gccggcgggc
     3721 cggtccaggt catctctccg gacatgactt ggaccctgcc gcgggtcgac ctctcgggcc
     3781 tggggggcga cgtggcgcgg cgcgagtcgg agcgcctcct cgccgaggag ggggagcggc
     3841 cgttcgatct ggagcgcggg ccgctgctgc gggccggcct cctccggcgc ggcgaggagg
     3901 atcacgccct tctcgtcaac atgcaccaca tcgtctccga cggctggtcg gtcggggtgc
     3961 tggtccgcga ggcctcggtt ctctacgcgg cggcgctggc cgggcggccg ctggagcggg
     4021 agctgcccgc gctgccggtc cagtacgccg acttcgcggt ctggcagcgc agctggctgc
     4081 agggggaggt gctggagcgg cagctcgcct actggcggga gcggctcgac ggcgcgccag
     4141 ccctcgattt gcccacggac cggccgcggc ccgccgccca gagcttccgc ggcgccaccg
     4201 tgcgcttcgc gctggagccc gcactcgtcc aggggctggc agcgctggcg cggcagcggg
     4261 acgccacgct gttcatggtc ctgctcgccc tcacgcagac cctgctcggc cgtcacgccg
     4321 gccaggacga cgtggtggtg gggggcggca tcgccaaccg caaccgggcc gagatcgagc
     4381 ctctcatcgg cttcttcgtc aacaacctgg tcctgcgcgc cgacctggcg ggcgatccga
     4441 cattccttga cctgctggcg cgtgctcggc gcgagacgct cggcggctac gcccaccagg
     4501 atctcccctt cgagcgcctg gtggaggagc tgcggccgga gcggcggctg tccgccaatc
     4561 cgctcttcca ggtcgcctgc gtgctgcaga atgcgccggc tgctcgaatc gagctgccgg
     4621 gcctgcggct gtcccccatg aatctcaggt cccgggcggc ccggctcgac ctcgagatgt
     4681 cgttcgacga ggcggacgga gggctggccg cggacttcta ctacgccacc gacctcttcg
     4741 atgaggcgac ggtgtggcgc ctgaccggtc atttccggac gctgctcgaa gccgcgctgg
     4801 aggagccggg actgcgactc tcggacctgc cgctcctcac cgcagcggag cggcagcagc
     4861 tcgtccacga gtggaacgac acgccgcggg agaccgtcgc ggcgccggcg ctccgcgaca
     4921 tcgtcgagga gcagctccgg ttgcggcccg atgccgtggc cgtggccgat gacaccggcg
     4981 tcctgaccta cggcgagctg gagcggcgct ccggccggct ggcccggacc ctggccgcgc
     5041 tgggagccgg ccccgaccgc cgcgtcgggg tctgcatgga gagcagcttc gagcgggtcg
     5101 tcgccatcct ggcggtgctg cgggccggtg gcgcctacgt gccgctcgac cccgcctacc
     5161 ctgccgagcg gctgacctat ctggtgagcg actccgacat ggctctgctt cttacccggg
     5221 agtgccggac cgagggcttc gaccccgcgc cggtcctggc tctggccgag gcggccgggg
     5281 tgaccgctct ggacgtggag gctgcgggcg aaccgctggt ggacgagggg tggatctcgc
     5341 cctcgctgct gccggagaac ctggcctacg tcatctacac gtcgggctcc acagggcggc
     5401 ccaacggcgt gatgatctcc cagggcagcg cagtcaacct cctgatggag acgctgccgt
     5461 tcctgcgctt cggccccgag gacagggtgg cccatgtcac ctcgttcagc ttcgatcctt
     5521 cggtggtcga gatcttcctc accctggcca ccggcggggc cctggtgctg gtgccggagg
     5581 aggagcggct ctcgcccctg gtactggcgg agcggctggc gcgccagcag gcgacgctcc
     5641 tggtcaccac tccggcgctg ctgtccctcc ttcccgcgga gcggctgccg caggttcgag
     5701 gcctgatcag tggcggcgac gcaccgtcgg cggatctggt ggaccgttgg gcacccgggc
     5761 ggcgattcat gaactgctac ggaccgacgg agatcaccgt ctacggcctg gccgagccct
     5821 gtcaggccgg agagggcgcg ccccccattg gacgtccact gggcaacgtc cgggcctacg
     5881 cgctggacca cgacctgcgg ccggtgccgg ccggggtgcc cggcgagttg gccgtgggcg
     5941 gcgcgggact ggcccgcggc tatctgggcc ggccggcccg cacggccgag cgcttcgtgc
     6001 ccgatccgct ggtcgcgacg ggcgagcggc tgtacctcac gggcgaccgc gtccgccagc
     6061 tccccgacgg acgcttccag ttcctcggcc gcgtcgacca tcaggtgaag ctgcggggga
     6121 tccgcgtcga gcccggagag gtcgaggcgg tcctggcgcg ccacccggag gtccgggagg
     6181 cggtggtggt gccgcgggag gagcctacgg gcaagcgcct ggtcgcctat gtggtgcccc
     6241 ggcccggggc cgcgcccgaa agcggcgacg gctccgagca ggtggagacc tggcggaccc
     6301 tctacgacca gacctatcag gagggcgacg gcgcctccga gttcgtgggc tggaacagct
     6361 cctacaccgg cgagcccatc cccgaggagg agatgcggga gtgggtcgag cgcacggtgg
     6421 agcggatcct ggccctctcc ccacggcggg tgctggagat gggatgcggc agcggcctgc
     6481 tgctgcggcg catcgccccg cgggtcgagc gctaccacgg cactgacttc tctcgctcgg
     6541 ccctggagcg gctgcggcgc gaggtggggg acctgccggg tgtctcgctc gatgtgcgga
     6601 cggccgacga ctggcgcggc atcgagcccg ggaccttcga cctggtggtg atcaactcgg
     6661 tggtccagta cttcccggac gccggctacc tgctgcgggt gctggagggc gccgtgcgcg
     6721 ccgtggctcc gggcggggtg gtgttcgtgg gcgatgtgcg gagccggccg ctgctggagg
     6781 cgcaggcggt gtcggtcgag ctgtaccggt cggcggagga tacccggcag ccggtctccc
     6841 ggttgcgcgg gtgggtggcc cggcgggtgg cgggggagga ggagctggta atcgatccgg
     6901 cgctcttcca cgcgctggtg gagcagttgc cggaggtctc cggcgtttcg actctggtca
     6961 agcgggggcg ggcccacaac gagctgacgc ggttccggta cgacgtggag atccgggtcg
     7021 gctcggcggg gccggcgtcg gaagagagcg aggggatcac cctggcgggg ctggccaacg
     7081 cccggctcgc cggagaggcg gcggcgatgg cggtcctggg tggtgcggag ctgaggtccg
     7141 tggaggagat gcgccgggcg atgaaggaga agagcgttcc ggcggtcgat cccgaggagc
     7201 tggcgcggct ggccgaaggg ttggggtatg cggtcgagct gcgggtggag ccgggggctc
     7261 ccttcgagtt cgccgcggtg ttgaggagcg ccgccctcgg agtggggtcc gggtaccctt
     7321 gctcggctcc ttcgggtgcg cccaccctcc cacccgccca gcgcgtccag tcgcctgcgc
     7381 agggtccccg gaccccactc cccccctccc ggtgggcgga gtggaccaat gatccgctgc
     7441 ggacgaagca ggaacggcgg ctgggaccgg agctgcggcg gttcctccag ggggagctgc
     7501 ccgaggccat ggtgccaggt gcgttcgtgt tcctggaggc cctgcccatc acggctcatg
     7561 ggaaggtgga ccgggcggct ctgccggatt tggaggtgga gagggttgga gtggagtttg
     7621 tcgcgcctcg gactcctctg gaggcccgga tcgtcgaggc gtgtgccgag gtgctgggac
     7681 tggaggcggt cggagtcggt gacaacttct tcgagctcgg cgggcactcg ctgctggcca
     7741 ctcagctcgt gtcacggctg cgggagcgct ggggcatcga gctgccggtc cgtgcggtgt
     7801 tcgaagcccg gacgctgggg gatctggccg agcgggtcac cggctgggag ccggccggca
     7861 ggatcccgcg ccggcccgag gggagcggtc cggcgccctt gtcgttcgct caggagcggt
     7921 tgtggttcct cgaccggatc gaggctggca cggcgtacag catgcccatg gcgctggcgc
     7981 tccggggcga gctgtcggtg ccggcgatgg aggcggcctt cggtgagctg gtgcgccggc
     8041 acgagaatct gcgcactact ttccatgatc tccaggaggg ggccgttcag gtcgtggagc
     8101 ctgcggggga ttggcggctc cttctggtgg atctgaccgg tgtcgcggag ggcagggagg
     8161 ctgaggtcgg gcacctgctc gccaccggcg cctggcgccg cttcgatctg gagaacggac
     8221 cgctgctgcg cgccgccctg ctgcgcaagg gggccacgga gcacctgctg atcctcgaca
     8281 tgcaccacat cgtctccgac ggctggtcca tgggcgtgct gatccgcgag gtctcggctc
     8341 tctacgccgc ggccctggcg ggccggccgt cgctgctgcc cgagctgccg atccagtacg
     8401 ccgacttcgc cgtctggcag cgcgcttggc tggaggccgg ggagctggag cgccagctcg
     8461 gctattggcg ggagcggctg gccggggcgc cggctctgga tctgcccacc gaccggccgc
     8521 gcccggcggt ccagagcttc cgcggcgcca cgctacgctt cgagatcgcg cccgaggaga
     8581 cccgcgggct ggagaccctg gcccggcagc gcgacgcctc gctctacatg gttctcctcg
     8641 ccctctccca gaccctcctc ggccgctaca ccggtcagga ggatctggtg acgggcgggg
     8701 ccatcgccaa ccgcaaccgc tccgagctgg agccgctcat cggcttcttc gtcaacagcc
     8761 tggtgctgcg cgccgatctg gccggggatc cgccgttcag cgatctcctc gaccgcaccc
     8821 ggcgcacgac cctggaggcc tactcccacc aggatctccc cttcgagcgt ctcgtcgagg
     8881 agctgcagcc ggagcggcgg ctatcccaca atccgctgtt ccaggtgatg tgcgtgctgc
     8941 agaacgctcc gggcgggcgg atcgacctgc cgggcctgcg gttcgagccg atgcagctcc
     9001 atttccccac cacgcggttc gacctcgaga tgatgttctg ggagcaggga gaggggctgg
     9061 gcggccactt cacctacgcg accgatctct tcgaagaggc cacggtacag aggatgatcg
     9121 gccatctcct caacctggct tcgggcgcca tcgagacccc cggccgtcgt ctctcggacc
     9181 tgccgctcct ggctccggcc gagcgctgcc agctcctcca cgagtggaac gacaccgccg
     9241 cgccgctccc ggacgccacc ctcctcgacc tcttccgcga gcaggtggcc caccggcccg
     9301 acgccatcgc cctctcctca cccgaaggcg acctcaccta cttcgccctg caccaccgcg
     9361 ccacccgtct ggcccatcac ttggccaccc ttggagccgg ccaggaggtc cccgtggccc
     9421 tcctcgctga ccgttccccc tccatgatca tcggcctgct ggccatcctc gaggccggag
     9481 gcgcctacct ccccctcgac ccctcgtatc caccggaccg cctggcccac atgcgcgaga
     9541 ccgccggggc cgatctcctc ctggccaacc ccgctctagt ccccgaagag ctccaccagg
     9601 ggactctcat cccccttacc catctcgggg agggggggac ctgggagagc gggcccggcg
     9661 caggcgactg gacgcgctgg gcgggcgggg aggggggcgc acccgaagga gccgagcagg
     9721 gcccgctccc ccaggtccgg ggggacaacc tcgcctacgt gatgttcacc tcgggctcga
     9781 ccgggcgccc caaggcgacc ggcgtcaccc accgcaacgt cgtccgtctc gtccgcgaga
     9841 gccgcttcgc cgacctgggc gcggaccagg tcttcctgca gctcgcgccc atctccttcg
     9901 acgcctccac tctcgaaatc tggggcgcgc tggccaacgg cggccgcctc gtcctggcgc
     9961 ccgccggtcg gaccgcgctc gaagacctgg cctccaccat cgagaccggg ggagtcacca
    10021 ccctctggct gacggccggc ctcttccacc agatggtgga cggccccatc gcctccctcc
    10081 gcggcctgcg ccagctcctc gcggggggcg acgtcctgtc gccggaccac gtccgccgcg
    10141 cc
//
LOCUS       DASPJI010000451         6004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_322847, whole genome shotgun sequence.
ACCESSION   DASPJI010000451 DASPJI010000000
VERSION     DASPJI010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322847"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3673
                     /locus_tag="VEL74_12230"
     CDS             <1..3673
                     /locus_tag="VEL74_12230"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HYG63342.1"
                     /translation="YAVAGAWSVPAQLVVAGTDGTAQLHRPRRSPLEAEALAAGYPKA
                     EIQADPGASSADLRLAAACTRSVVVTTQDRAPGQGEAPPPRPVEGALVTADSWTLGVT
                     DAAGSLAVPAPCDRELRAEVMTRDGRRAEVTLPRGPAGASPVAVRLSPLPARLSGKVV
                     ENGSRKPVAGALVWPSDDPAAFVLTDAKGAYTIKAPAGDAPRVDGAAAGYLPAMETAR
                     AAAGPTLSLKPLATVSGSVVDAAGRPVAEAVIRAVTEGGGARAGRGSASSAGLVTRSG
                     PQGLFQLRVVPGHPVTLVASHPDFTQAKLPLPELAARASRTNLRIVLEKGLSGFGKVV
                     DAAGRPVSGAEVALIPSGEERDPFARAFMMDNPPAGPQAFTDAQGSFLLEHLEPGRFS
                     LHTSARGFAPVTVPGIQLAAGQPRVDLGTVTLAPGATLEGVVVDPRDRPIAGAQVGVR
                     AVTRTARHIAEPEEDILTSADGSFSIPDLKPGEKVMLFVSKLGYVRATFNGIEVPPVT
                     PLRVVLTPGVRVAGRVVDEGGDPVAGASVRLTPAGTTMVTSRGPMESMRGFAVSETDG
                     RFAIEGVEAGKVRLVATAQGFLEGEVPGFDVVTGRDIDDLEVVLRRGSVVTGRVTGPD
                     GAPVSGARVQMVEEAGRGSYRMGGSASSDGDGNYRLEGVPEGAHSFAAQGEGLQRAVR
                     DLEVRTGENRLDFRLEGGHEVSGRVVDSSGQPAAGAMVRLSSTGDRIPTMSRSGRPEA
                     RSGADGSFRISGVATGVYDVAADKEGYAPAQLESVNVTGRVQGLELRLEAGGGIQGRL
                     LGLDFSELAGASVYANPAEPALARTVMRLARAGQVDYQGQYRISGLTAGEWRVSARTD
                     SGRQAEGKVTVTPGAEATLDLELGGGFTLTGRVLRGGEGVPNIDVLMHGTGEGGGSGR
                     ASTDPQGAFRIQGLKPGTYQLQAMSFRTGLSHNEAMEISGDRDILIELPTRRVSGRVV
                     DATDQSPIANVVVSLEPADGAEIRSGFGKGSRTDADGAFTISEVAPGSYRVNARLDGY
                     APAESPVQVDNDDDVDGVRLALQPTQGLTLEVRSSLGTPPSEVRVALLDPSGRSVLSG
                     THTTGENGRARVSSAPPGRWRLLVASGASATTALDVQVPGPPIPVVLAPGGMLTVLAG
                     GMPTGTIISLVGSGGQPFQSLMFGGGVRSQWSLNEGAVVIEGVPPGQWRVRATTLDGQ
                     AREGTASVSSGGASQVTLR"
     gene            3763..3966
                     /locus_tag="VEL74_12235"
     CDS             3763..3966
                     /locus_tag="VEL74_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HYG63343.1"
                     /translation="MTFMNLSAGERVIRVVLGLLMLAAGWSGWVPGVWAVALKAFGWV
                     PLLTGLIGWCPFYALLGIRTAKL"
     gene            4013..5449
                     /locus_tag="VEL74_12240"
     CDS             4013..5449
                     /locus_tag="VEL74_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HYG63344.1"
                     /translation="MSEPASSIPHESSRPRDAGPSESAVTLRRLRWMAILVALVMAVL
                     LDQSRQAFYPFLRDWPGRILQGTLVLFSLIFLIGALFHVIDKMQRKLERQNRELLALH
                     EAGLDVHSELALEPLFQKVVDQARLLLDARYGALSLIDGQNRIEVFTVSGITPEQRAR
                     LGDPPVGHGLLGVVLHEGHRLRLPDISKDPRSVGFPPNHPPMRSLLAVPLPCKGPFRG
                     NLYLAEKQGEPEFTGEDEETLTRFAVKVALAIDNAHLHRQLNTLAVAEERMRIAHEMH
                     DGLAQVLAYVNTKAQAVREFLRGGRNEEAERHLEQLAAAAREIYADVRESIIGLRSAG
                     SGRTMSEAMAEYVASWERQAGIECDLAVDPELRLAAGPELQLLRIVQEAMANVRKHAA
                     AKRVEVRIERLDGRVVATVEDDGAGFNPAETRRSEFPRFGLATMRERAESIGGSVQLD
                     TAPGAGTRVRVEIPYPAPNQSPAEGRTS"
     gene            5446..>6004
                     /locus_tag="VEL74_12245"
     CDS             5446..>6004
                     /locus_tag="VEL74_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYG63345.1"
                     /translation="MRILIADDHALFRDSLKSLLCARGLEVLGEARDGKEAVELARRL
                     QPDVVLMDLAMPNVDGLSATKLISAEMPQVKVVVLTASDEDAKLFEAIKSGAQGYLLK
                     NLESEEFFALLEGVGRGEPALTPSLARKLLQEFARPTQAPAPPQDPDALTDREREVLE
                     LLVRGVTSNRKLAKQLGVSENTVKFH"
BASE COUNT          808 a         2217 c         2164 g          815 t
ORIGIN      
        1 gtacgcagtc gccggcgctt ggtcggttcc ggcccaactc gtggtcgcgg gcacggacgg
       61 caccgcccag ttgcaccgcc cgcggcgcag ccctctcgag gccgaagccc tggccgccgg
      121 atatcccaag gccgagatcc aggccgatcc cggcgcatcg tcggccgatc tgcgcctcgc
      181 cgccgcctgc acgcgctccg tcgtggtgac gacccaggac cgggcgccgg gccagggtga
      241 agcgcctccc ccccggccag tcgaaggggc cctggtgacg gcggattcct ggacgctggg
      301 cgtcaccgac gcggcgggca gtctggcggt ccccgcgccc tgcgaccgcg agctgcgggc
      361 cgaggtgatg acccgggatg gccgccgcgc cgaggtcacc ctcccccgcg gcccggccgg
      421 ggcctcgccc gtcgccgtca ggctgtcgcc gctgccggcg cggctgtccg gcaaggtggt
      481 cgagaacggc tcccgcaagc cggtcgccgg ggccctggtc tggccgagcg acgatccggc
      541 cgccttcgtc ctcaccgatg ccaagggtgc gtacaccatc aaggcgccgg ccggcgacgc
      601 cccgcgcgtc gacggcgccg cggcgggcta cctccccgcc atggagacgg cacgggcagc
      661 ggccggtccc accctctccc tgaagccgct cgccacggtg agcggctctg tggtggacgc
      721 ggcgggccgg ccggtcgccg aggcggtgat ccgggccgtc accgagggcg gaggggcccg
      781 ggccggccgc ggcagcgcct ccagcgccgg cctcgtgacc cgcagcgggc ctcaggggct
      841 cttccagctc cgcgtcgtac cgggccaccc cgtgaccctc gtcgcttcgc atccggactt
      901 cacccaggcc aagctccccc tccccgagct ggcggcgcgg gcgtcccgga cgaacctccg
      961 gatcgtcctc gaaaaggggc tgtccggctt cggcaaggtc gtcgatgccg ccggccggcc
     1021 ggtgagcggc gccgaggtgg ctctgatccc ttccggcgag gagcgggacc ccttcgcgcg
     1081 cgccttcatg atggacaacc cgccggcagg gcctcaggcc ttcaccgacg cccaggggag
     1141 cttcctcctg gagcacctgg agcccggccg gttctcgctg cacacctccg cccgcggctt
     1201 cgcgcccgtc accgtgccgg gtatccaact cgccgccggc cagccgaggg tcgatctcgg
     1261 caccgtgacc ctggcgccag gcgcgacgct ggaaggggtg gtggtggacc cgcgggaccg
     1321 ccccatcgcc ggcgcccagg tcggcgtccg cgccgtgacc cggaccgcgc ggcacatcgc
     1381 ggagcccgag gaggacatcc tcacctcggc ggacggctcc ttctccatcc ccgacctcaa
     1441 gccgggcgag aaggtgatgc tcttcgtctc caagctgggg tatgtccgcg ccaccttcaa
     1501 cggcatcgag gtgccgcccg tcaccccgct gcgcgtcgtg ctgacgccgg gcgtgcgggt
     1561 ggccggccgc gtcgtcgacg aagggggcga tcccgtggcc ggcgccagcg tgcggctgac
     1621 cccggccggc accaccatgg tgacgtcccg gggaccgatg gagagcatgc ggggcttcgc
     1681 ggtctcggag accgacggcc gcttcgccat cgagggggtc gaggcgggca aggtccggct
     1741 cgtcgccacc gcccagggct tcctggaggg agaggtgccg gggttcgacg tggtcaccgg
     1801 cagagacatc gacgacctcg aggtggtcct ccggcgcggc tcggtggtca ccggccgcgt
     1861 caccggcccg gacggggcgc ccgtttccgg cgcgcgggtg cagatggtgg aggaagccgg
     1921 ccggggcagc taccgcatgg gaggctcggc cagctcggac ggcgacggca actatcggtt
     1981 ggaaggggtc cccgaggggg cccactcgtt cgccgcccag ggggagggcc tccagcgcgc
     2041 ggtgcgggat ctggaggtcc ggaccgggga gaaccgcctg gatttccggc tggagggggg
     2101 gcacgaggtc tccggccgcg tggtggattc ctccggccag cccgcggcgg gtgccatggt
     2161 ccgtctgagc tccaccggcg acaggatccc caccatgtcg cgcagcggcc ggccggaggc
     2221 gcgtagcggc gccgacggct ccttccggat ctccggcgtc gccaccggcg tctacgacgt
     2281 cgcggccgac aaggagggct acgctccggc gcagctcgag tcggtcaacg tcaccggccg
     2341 ggtgcagggg ctggagctgc gcctggaggc gggcggcggc atccaggggc gtctcctcgg
     2401 cctcgacttc tccgagctgg ccggcgccag cgtctacgcc aaccccgccg aaccggccct
     2461 cgcccgcacg gtcatgcgcc tggcgcgcgc cggccaggtg gactaccagg ggcagtaccg
     2521 gatctccggc ctgaccgcgg gcgagtggcg ggtcagcgcc cgcaccgaca gcggccggca
     2581 ggcggaggga aaggtcacgg tcaccccggg agccgaagcc acgctcgacc tggagctcgg
     2641 cggcggcttc acgctgaccg gccgcgtcct gcgaggcggc gagggggttc ccaacatcga
     2701 cgtcctgatg cacggcaccg gggagggcgg aggctccggc cgggcctcca ccgacccgca
     2761 gggggcgttc cgcattcagg ggctcaagcc cggcacctac cagctccagg ccatgtcctt
     2821 ccgcaccgga ctctcccata acgaggcgat ggagatctcg ggcgaccggg acattctcat
     2881 cgagctcccc acccgccggg tgagcggccg ggtggtggac gccaccgacc agtcgcccat
     2941 cgcgaacgtc gtcgtctccc tggagccggc ggatggcgcc gagatccgca gcggcttcgg
     3001 caagggcagc cgcaccgatg ccgacggggc cttcaccatc tccgaggtcg cccccggctc
     3061 ctaccgggtg aacgcccgac tggacggcta cgcgcccgcc gagagcccgg tccaggtgga
     3121 caacgacgac gacgtggacg gcgtgcgcct cgccctccag cctacccagg ggctcacgct
     3181 ggaggtgcgg tcctccctgg ggacgccgcc gtcggaggtc cgggtggcgc tgctggaccc
     3241 ctccggccgt tcggtcctct ccggcaccca caccactggc gagaacggcc gcgcccgcgt
     3301 gtccagcgct ccaccgggac gctggcgcct cctggtggcc tccggcgcca gcgccaccac
     3361 ggcgctggac gtccaggtgc ccggcccccc catcccggtc gtgctggccc ccggcggcat
     3421 gctgaccgtc ctggccggcg gcatgcccac cggcaccatc atcagcctcg tggggagcgg
     3481 cggccagccc ttccaatccc tgatgttcgg cggcggtgtc cgctcccagt ggagcctcaa
     3541 cgaaggagcg gtcgtgatcg aaggggtgcc gcccgggcag tggcgggtgc gggccaccac
     3601 gctcgacggc caggcgcgcg agggcaccgc cagcgtgtct tcgggaggag cgtcacaggt
     3661 cacgctgcgg tagatcccca ccctgcgggg tggggcgggc cccagagcct ccacaccccg
     3721 ccgggtgtag cggtggcgcg acgcggccgg taccctccgg ccatgacgtt catgaacctg
     3781 tccgccggcg agcgcgtgat ccgtgtcgtc ctcgggcttc tgatgttggc cgccggatgg
     3841 agcggctggg tacccggggt ctgggcggtc gccctcaagg ccttcggctg ggtccccctg
     3901 ctgaccggcc tgatcggctg gtgccccttc tacgcgctgc tcggtatccg gaccgccaag
     3961 ctttgagccc gccgctcctc cgccggggaa atccgttagg atctcctgcg ggatgtctga
     4021 gccggcatca tccattcccc acgagagctc gcggccgcgc gacgccggtc cctcggagtc
     4081 ggcggtgacg ttgcgccgcc tccggtggat ggccatccta gttgccctgg tgatggccgt
     4141 cctcctcgac cagtcccgcc aggccttcta tccctttctc cgcgactggc cgggccgcat
     4201 cctgcagggg accctggtcc tgttcagcct gatcttcctg atcggggccc tgttccacgt
     4261 catcgacaaa atgcagcgca agctcgagcg gcagaaccgc gagctgctgg cgctgcacga
     4321 ggcggggctc gacgtgcaca gcgagctggc cctggagccg ctcttccaga aggtcgtgga
     4381 ccaggcgcgc ctcctcctgg acgcccgcta tggcgcgctg tccctgatcg acggccagaa
     4441 ccgcatcgag gtcttcaccg tctccggcat cacccccgag cagcgcgccc ggctcggcga
     4501 cccgccggtg gggcacggcc tgctcggagt ggtgctgcac gaggggcacc ggctgcggct
     4561 gccggacatc agcaaggatc cgcggtcggt gggcttcccg ccgaaccatc caccgatgcg
     4621 ctcgctgctc gcggtaccgc taccctgcaa gggacccttc cgcggcaatc tctatctggc
     4681 ggagaagcag ggggagcccg agttcacggg cgaggacgag gagacgctca cccgtttcgc
     4741 ggtcaaggtc gccctggcga tcgacaacgc ccacctgcac cggcagctca acaccctggc
     4801 ggtggccgag gagcggatgc gcatcgccca cgagatgcac gacgggctgg cccaggtgct
     4861 ggcctacgtc aataccaagg cgcaggcggt gcgggagttc ctgcgcggcg gccgcaacga
     4921 ggaggcggag aggcacctgg agcaactcgc cgccgcggcg cgcgagatct acgccgacgt
     4981 gcgggagtcg atcatcggcc tgcgctccgc cggctccggg cggaccatga gcgaggccat
     5041 ggccgagtac gtggcgtcgt gggagcggca ggctggcatc gagtgcgacc tcgccgtcga
     5101 tcccgagctg cgcctcgccg ccgggccgga gttgcagctc ctgcgcatcg tccaggaggc
     5161 catggccaac gtccgcaagc acgccgcggc gaagcgggtc gaggtacgga tcgagcggtt
     5221 ggacggccgg gtggtggcca cggtggagga cgatggcgcc ggcttcaatc cggccgagac
     5281 gcggcgctcg gagttccccc ggttcggcct cgccaccatg cgcgagcgcg ccgagagcat
     5341 cggcggctcg gtccagctcg acaccgcccc cggcgccggg acccgggtgc gggtcgagat
     5401 accctatcca gcaccgaacc agagcccagc cgaggggaga acctcatgag aatcctgatt
     5461 gccgatgacc acgccctctt ccgcgacagc ttgaagagcc tcctctgcgc ccgcggcctg
     5521 gaggtgctgg gagaggcccg cgacgggaag gaggccgtgg agctggcgcg ccggctgcag
     5581 ccggacgtgg tcctcatgga cctggccatg cccaacgtcg acggcctctc ggccaccaag
     5641 ctgatcagcg ccgagatgcc gcaggtcaag gtggtcgtgc tcaccgcctc ggacgaggat
     5701 gccaagctct tcgaggccat caagtcggga gctcagggtt acctgctgaa gaacctggag
     5761 tcggaggagt tcttcgcgct gctcgagggg gtgggccggg gggagccggc gctgacgcct
     5821 tcgctagccc gcaagctcct ccaggagttc gcccgcccca cccaggcgcc ggcgccgccg
     5881 caggaccccg acgccctcac cgaccgcgag cgcgaggtgc tggagctgct ggtgcgcggc
     5941 gtcacctcga accgcaagct ggcgaagcag ctcggcgtca gcgagaacac cgtcaagttc
     6001 cacg
//
LOCUS       DASPJI010000452         2892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_538864, whole genome shotgun sequence.
ACCESSION   DASPJI010000452 DASPJI010000000
VERSION     DASPJI010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538864"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VEL74_12250"
     CDS             complement(<1..291)
                     /locus_tag="VEL74_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYG63346.1"
                     /translation="MRYTPRVRRWTLLAPLSLLLTSCASLRPFEEVRRQVPAEEFVEV
                     DGGLVHVEQAGSGDAADAVVLLHGFGASSYGWRKVMPGLAESYRVVAPDFYGF"
     gene            417..1037
                     /locus_tag="VEL74_12255"
     CDS             417..1037
                     /locus_tag="VEL74_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63347.1"
                     /translation="MILLAFLGAALPAAAQDGWVFQASLGAVANLETPLTIRQSGFEE
                     IETGGAEYETRPFESPVYYSLRAGRWRGGVGWELELIHHKIYLQDPPPEVQHFAISHG
                     YNMVTVNRAWERGGFVWRAGLGPVVAHPENQIRGRVLRDAGYELTGPVVQAGVERRFV
                     LGERWFVSLEGKATAARAEVSVADGEAEVPNAALHGLLGIGYRWRR"
     gene            1069..1659
                     /locus_tag="VEL74_12260"
     CDS             1069..1659
                     /locus_tag="VEL74_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63348.1"
                     /translation="MPDLKKWKLQGLWVLACALTIQGCASSGRAGGAGDQVITATLAD
                     VGNTLQGTTTEPVVIHIEQFSSPQEVQQLANLLAEKGPSAVQDATWRLEKGWIRVGGS
                     LGYPLAVITRRPTSEGQRITILLDRPVTYREQWQNLRSADYPFGYIELNLDRNGRGDG
                     QFIPAGQIRLSSTGALEVVQYGFQPVRLLNVQVQRG"
     gene            complement(1672..>2892)
                     /locus_tag="VEL74_12265"
     CDS             complement(1672..>2892)
                     /locus_tag="VEL74_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63349.1"
                     /translation="ETVLNASLALARKARELTGLGTVEVVVADPEPRVALAATGEAQA
                     LSDALGAIAAQARQDGSRAAKANKAGGSVAPETAALRRQLDRLVTLVADRPRTGAGAL
                     FLVMDAFVPPPGEADLLSTALADDPGPPGTATAALRETARVLAARGLVTFAVPMREPQ
                     ERKRVEVNDTERIRVMSGPSRHTTGVPPVIPMGGTGRGPLRHEPVVDVLTRPDSAPLM
                     TLVQPTAGTVLGVEEQLGPALQGLAGRWRVWFQAPEIPDGSLRRVEVRLPSSKVPLRG
                     PRWVMASTPEGLAAARARLLAGGTSMTGGAVPVEARLEGKELRLRLPALPATAAAPAG
                     PVRISVAFDNRSEVKHTVVPEADLGKAWQTTLRIQPPTGARKAAVVVEDLARGRWGAT
                     ALDLTAAPAAQPGG"
BASE COUNT          418 a          983 c         1093 g          398 t
ORIGIN      
        1 gaagccgtag aagtccggcg ccaccacccg gtaggactcc gcgaggcccg gcatcacctt
       61 gcgccagccg tagctcgacg cgccgaagcc gtgcagcagc accaccgcat cggccgcgtc
      121 gccgctccct gcctgctcca cgtggacgag cccgccgtcc acctcgacga actcctccgc
      181 cggcacctgc cggcggacct cctcgaaggg ccggagcgag gcgcaggagg tgagcagcag
      241 agagagcggg gcgaggagcg tccagcggcg cacgcgggga gtgtagcgca tgccccgccg
      301 cggtgttggc ggcgtgggcg gcgttggcgt gtgtcctgca ggctttcatg gatgaagggc
      361 tcgccttcgg gtgcgccggg gagaaggagc tcctggtgcg acgatccata cgaaaaatga
      421 tcctcttggc tttcctggga gccgccctgc cggcggcggc gcaggacggg tgggtctttc
      481 aggcctctct cggcgcggtg gccaatctgg agacgccgct caccatccgc cagtccggtt
      541 tcgaggagat cgagaccggc ggcgccgagt acgagacccg gcccttcgag agcccggtct
      601 actactccct ccgggcgggc cgctggcggg gcggcgtcgg gtgggagctg gagctgatcc
      661 atcacaagat ctatctccag gacccgccgc cggaggtgca gcacttcgcc atctcccatg
      721 gctacaacat ggtgacggtg aaccgcgcct gggagagggg cgggttcgtc tggcgggccg
      781 gcctgggccc ggtcgtggcc cacccggaga accagatccg cggccgggtg ctgagggacg
      841 ccggctacga gctgaccggt cccgtcgtcc aggccggggt ggagaggcgt ttcgttctgg
      901 gagagcgctg gttcgtgtcg ctcgaaggca aggcgacggc ggcccgcgcc gaagtatcgg
      961 tcgcagacgg cgaggcagag gtacccaacg cggctttgca cgggctcctg ggtatcgggt
     1021 accgctggcg ccggtgaagt gaaacatcga aaggcgaagg gggacgtcat gcccgatttg
     1081 aaaaagtgga aattacaagg actgtgggtg ctcgcgtgtg ccttgacgat ccaggggtgc
     1141 gcttcgagcg gcagggccgg cggggccgga gatcaggtca tcaccgcgac cctggccgat
     1201 gttggaaata cgctccaggg caccacgacc gagccggtcg tgatccacat cgagcagttc
     1261 tccagtccgc aggaggtcca gcagctcgcg aacctgctgg cggaaaaggg gccgagcgcc
     1321 gtgcaggacg cgacctggag gctggagaag ggttggatcc gcgtcggtgg cagtctcggt
     1381 tacccgctgg cggtgatcac caggcgtccg acgtccgagg ggcagaggat cacgatcctc
     1441 ctggaccgcc cggtcacgta ccgcgagcag tggcagaacc tgcgctcggc ggactatccc
     1501 ttcggctaca tcgagctgaa cctggaccgg aacggccggg gtgacgggca gttcatcccg
     1561 gccgggcaga tccgtctgag cagcaccggt gccctggagg tggtgcagta cggtttccag
     1621 ccggtccggc tgctcaacgt ccaggtccag cgcgggtagg ctcggcccgc atcaacctcc
     1681 cggttgcgcc gcgggagccg cggtcaggtc gagcgccgtg gctccccagc gtccgcgggc
     1741 caggtcctcg accaccacgg cggccttgcg ggcgccggtg gggggctgga tccgcagcgt
     1801 ggtctgccag gctttcccga ggtcggcctc gggtacgacg gtgtgcttca cctccgagcg
     1861 gttgtcgaag gccaccgaga tgcggaccgg gccggcgggg gcggccgcgg tcgcaggcaa
     1921 cgccggcagc cgcaggcgca gctcctttcc ttccagtctg gcttcgaccg gcacggcgcc
     1981 gcccgtcatc gaggtgccgc cggccagcag ccttgcccgg gcggcggcga gaccttcggg
     2041 cgtcgaggcc atgacccagc gcgggccgcg cagcgggacc ttggacgagg ggagccggac
     2101 ctcgacccgg cggagcgagc cgtccgggat ctcgggtgcc tggaaccaga ccctccagcg
     2161 cccggcgagc ccctggaggg cggggccgag ctgctcctcg acacccaaca ccgtgccggc
     2221 cgtgggttgg accagggtca tcagcggcgc cgagtccggc cgcgtcagga cgtcgaccac
     2281 gggctcgtgc cgcaaaggcc cgcggccggt gccgcccatg gggatgaccg gcggtacgcc
     2341 ggtggtgtgc cggctggggc cggacatgac gcggatgcgc tccgtgtcgt tgacctccac
     2401 ccgcttgcgc tcctgcggct cccgcatcgg caccgcgaac gtcaccaggc cccgcgccgc
     2461 gaggacgcgg gccgtctcgc gcagcgcggc ggtggccgtc cccggtgggc cgggatcgtc
     2521 ggccaaggcg gtggagagga gatcggcctc gccgggcgga ggcacgaagg cgtccatcac
     2581 caggaacagg gcgccggcgc cggtgcgcgg ccggtccgcg accagagtga cgaggcggtc
     2641 gagctggcgg cgcagggcgg cggtctcggg cgccacggac ccgccggcct tgttcgcctt
     2701 ggcggcccgc gagccgtcct gccgcgcctg ggcggcgatg gcgccgaggg catcggaaag
     2761 ggcctgggcc tcgcccgtgg ccgcgagggc cacccgcggc tcggggtcgg ccaccaccac
     2821 ctccaccgta cccaggccgg tgagctcgcg ggccttccgg gccagcgcca gggaggcatt
     2881 cagcaccgtc tc
//
LOCUS       DASPJI010000453         5056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_430169, whole genome shotgun sequence.
ACCESSION   DASPJI010000453 DASPJI010000000
VERSION     DASPJI010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430169"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1509)
                     /locus_tag="VEL74_12270"
     CDS             complement(<1..1509)
                     /locus_tag="VEL74_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63350.1"
                     /translation="MRFRGLSALSLLLLTAAVVPGLPVRGDSQPAQFLLDEAPRCDET
                     APDVAFLPGGGFVAVWGGSRPDALLASSAVYLRVFDRSGRPLGPETMLTGDADLAGRP
                     AVAADADGTVMVVWGDDRGLEGALKGRLLSPAGAPLGGVFEIGPRFPEGGVDVTAETG
                     GGFAVVWADGGAIYLRRYTVAGEPRGPLEVVIASSGIPEDPPSRTYDPAVVPHGSGLA
                     ITYIDGFQRDWGDPALSGANLGLIYWRPGEGLLFPEVGELGFDPVPAYDQRLPRLALA
                     VGAAGRVFVAWREQTPENGLLLRGRLYTALAEPLGPAFRIDFGDWDHLSGLSVAADGA
                     GNFLVAWLGARAIPQLPGAEAYLRRFGPDGHPLGGQEPVSARFTFFDPTTSPGLAVDP
                     AGRALVAWGTGGRQLPPHVGAPHQCFSQGLFARFLDAPVPLDLPLQGGRFLAGVRWAD
                     HAGRTGVGQPVALTGDSGYFWFFDENNAELVVKVLDGRAVNGHYWVFYGALSD"
     gene            complement(1525..3498)
                     /locus_tag="VEL74_12275"
     CDS             complement(1525..3498)
                     /locus_tag="VEL74_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63351.1"
                     /translation="MRSIYKVLATTCLLTVAAGHAAAGAENLRLDTGSACQERRPDIA
                     AFSDGRFVAVWQQGAEPETISQPRIFARLFGESGTLPDPEFLVSGTLNAGRSPEVAVN
                     NSDQFMVAWLDSSGKVRGRLYGPTGAVLGPDRGLSSEAANGFALTATNDGNFALVTAH
                     DDSVVLLRLGTVGQVLAPPQVISSVTFPIAPHPTYEDPAVAPYGASGVAVAWAYEDLD
                     PRTRSLVFGVIPSPAASFEPVAEVVEPFGEDDLPRPRPAVAANATGQVLAAWNRQGRP
                     GAGTVLRAQVFRTDGIPLGSPIEFGGWDVASVSAPDAVATPEGDFLLAWQHYTGGPAL
                     SIGSVARIEANGQPAGRVFNAWNTWGFEGPSLTLDSGGQALVAWQTGDHLEGNAEACF
                     SSGLYARRIAVSSDVLPLRDGRFEVRVHWTDHSGFSGEGRATQLTADSGSFWFFGPEN
                     VELMVKVLDGWEINGHFWVFYASLTDVAFTLEVTDTATGLTRTYENPAGQLASRADTS
                     AFPTGTRAAAGTDTVAATASVAARSHVPDGSGAGPCSPPDLPVVPRPGLCLGGRFEVE
                     VAWHIPVTSGAGQGVRLTADSGYLWFFWPDNIELVVKVLDGRAVNGHFWVFYGALSDV
                     EYTVLVRDTLTGQVRTYHNPAGQLRSRADTAAF"
     gene            complement(3514..>5056)
                     /locus_tag="VEL74_12280"
     CDS             complement(3514..>5056)
                     /locus_tag="VEL74_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63352.1"
                     /translation="LTATHDGGFAIVTTMEELVILWQIGPTGQPPVASRALEWPSGPR
                     SLFADPAVAPYGPSGVVAAWVREDQGQMTRTLVFGIAPPGTAPAEPVLEVVEPYGLGD
                     LPRPRPAVAANAAGQVLAAWNQQGSPGVGAVLRAQVFAADRQPVGPRLEFGGREVESV
                     SAPEVLAAPGGDFLLSWQRLTSGPALAIGAVAHIEPDGALVEPVFNAWNTWSFEGPAL
                     TLGSRGEALVAWQTGDLLPGDAETCFSSGLYARQIPTSPGVLFLRDGRFEARVHWTGH
                     TGGSGGGYAAQLTPDATPDAGSFWFFGQENVELLVKVLDGRAVSGSFWVFYASLTDVA
                     FTLEVTDRVTGQTRTYENPAGRLASFADTAAFRATAAAGAGTASTAPRFLFPDDSGEE
                     EPTEGPCTPPELQRYSRRPGLCLDGRFEVEVAWTANGGTGDAEGVSLSSDSGYLWFFD
                     PDNIELVVKVLDGRAVNGHFWVFYGALTDVEYTVLVRDTETDEVKTYHNPAGQLRSRA
                     DTAAF"
BASE COUNT          787 a         1803 c         1859 g          607 t
ORIGIN      
        1 gtccgagagg gcgccgtaga agacccagta gtggccgttg accgcgcggc cgtccagtac
       61 cttgaccacc agctcggcgt tgttttcgtc gaagaaccag aaatagccgg agtcgccggt
      121 gagggccacg ggctgcccca ctccggtgcg gccggcgtgg tcggcccagc gcaccccggc
      181 caggaagcgg cccccctgga gcggcaggtc gagagggacg ggggcgtcca ggaaccgcgc
      241 gaagagcccc tggctgaagc actggtgagg agcgccgacg tgcgggggca actgccggcc
      301 gccggtcccc caggcgacga gcgcccggcc ggccgggtcc accgcgagcc ccggcgaggt
      361 ggtcgggtcg aagaaggtga agcgggccga aaccggctcc tgccctccca ggggatggcc
      421 gtccggaccg aagcgccgga ggtaggcctc ggccccgggc agttggggga tggcccgtgc
      481 cccgagccag gccaccagaa agttgccggc gccgtcggca gccaccgaca acccggagag
      541 atgatcccag tcgccgaagt cgatgcggaa ggcgggaccc aggggctcgg cgagggccgt
      601 gtagagccgg ccacgcagca ggaggccgtt ctccggcgtc tgctcccgcc aggcgacgaa
      661 gacgcggccg gcggccccca ccgccagggc gaggcgaggg agccgctggt cgtaggccgg
      721 cacgggatcg aagccgagct cgccgacctc cgggaagagg aggccctcgc cgggccgcca
      781 gtagatcagg cccagattgg cccccgacag ggccgggtcg ccccagtccc gctggaaccc
      841 atcgatgtag gtgatcgcca agccggagcc gtgaggcacg acggcggggt cataggtccg
      901 ggacggcgga tcttcgggga tgccggaaga ggcgatgacg acctccaggg gcccccgtgg
      961 ctcgcccgct acggtgtagc ggcgcaggta gatcgcgccg ccatcggccc agaccaccgc
     1021 gaagccgccg cccgtctccg cggtcacgtc cacgcccccc tccgggaacc gcggcccgat
     1081 ctcgaagacg ccgccgaggg gcgcccccgc ggggctgagc agccggccct tcagggcgcc
     1141 ctccagtccc cggtcatcgc cccacaccac catgaccgtg ccgtccgcat cggccgccac
     1201 ggcgggccgg cccgccaggt ccgcgtcgcc ggtcagcatc gtctcgggac ccagcggccg
     1261 gccgctccgg tcgaagaccc gcagatagac cgccgagctc gccagcaggg cgtcgggacg
     1321 cgagccgccc cagaccgcca cgaagccgcc gccgggcagg aaggccacgt ccggcgccgt
     1381 ctcgtcgcag cgcggcgcct cgtcgaggag gaactgcgcg ggctgtgagt ccccccggac
     1441 cggcaggccg gggacgacgg ccgccgtcag gagcaggagc gagagcgccg acagtccacg
     1501 gaatcgcatg acatccctcc agtctcagaa cgccgcggtg tcggcgcggc tgcggagctg
     1561 gccggccggg ttgtggtagg tccgcacctg gccggtcagg gtgtcgcgca ccaggacggt
     1621 gtactcgacg tcgctgagcg ccccgtagaa gacccagaag tggccgttga ccgcccggcc
     1681 gtcgagcacc ttgaccacca gctcgatgtt gtctggccag aagaaccaga gataaccgct
     1741 gtccgccgtc aggcggaccc cctgcccggc gcccgaggtc acggggatgt gccaggccac
     1801 ctccacctcg aagcggccgc cgaggcagag gccgggccgg gggaccaccg gcaggtccgg
     1861 cggggagcag gggccggcgc cgctgccgtc gggaacatgg gagcgcgccg ccacggaggc
     1921 ggtcgccgcc acggtgtccg tccctgccgc ggcgcgcgtc cccgtcggaa aggccgaggt
     1981 gtcggcgcgg ctggcgagct ggccggccgg gttctcatag gtcctggtca gcccggtggc
     2041 ggtgtccgtc acctcgaggg tgaaggcgac gtcggtgagg gaggcataga agacccagaa
     2101 gtgaccgttg atctcccagc cgtcgagcac cttgaccatc agctcgacgt tctccgggcc
     2161 gaagaaccag aaggagcccg agtcggcggt gagctgcgtg gcgcgcccct cgccggaaaa
     2221 gccggagtgg tcggtccagt ggacccggac ctcgaagcgg ccgtcccgga gggggagcac
     2281 gtccgaggag accgcgatcc gccgcgcgta gagcccgctg ctgaagcagg cctccgcgtt
     2341 gccctccagg tgatcgcccg tctgccaggc gacgagggcc tgcccgccgg agtccagggt
     2401 gagcgagggg ccctcgaagc cccacgtgtt ccaggcgttg aacacccgcc ccgcgggctg
     2461 gccgttggcc tcgatgcggg cgaccgaccc gatgctgagg gcgggcccgc cggtgtagtg
     2521 ctgccaggcc agcaggaagt ccccctccgg cgtcgccacg gcgtccgggg cggagacgct
     2581 cgccacgtcc cagccgccga attcgatcgg ggagccaaga gggatcccgt ccgtgcggaa
     2641 gacctgggcc cggagcaccg tcccggcccc cgggcggccc tgccggttcc aggccgccag
     2701 gacctgtccg gtggcgttcg cggccaccgc gggacgggga cgcggcaggt cgtcctcgcc
     2761 gaagggctcg accacctccg ccaccggctc gaaggacgcc gcgggcgagg gaatgacccc
     2821 gaagacgagg gaccgcgtcc tcggatccag atcctcgtac gcccaggcca ccgccacccc
     2881 ggacgccccg tagggcgcca ccgccggatc ctcatacgta ggatgcggcg cgatggggaa
     2941 ggtcacggac gagatcacct gggggggcgc caggacctgc cccacggtgc cgagccgcaa
     3001 caggacgacc gagtcgtcgt gcgccgtcac cagcgcgaaa ttgccgtcgt tcgtcgcggt
     3061 cagagcgaag ccgttcgcgg cctcggagga gagtccccga tcgggcccga ggacggcacc
     3121 ggtagggccg tagagccggc cccggacctt ccccgagctg tccagccagg cgaccatgaa
     3181 ctggtcggaa ttgttgaccg ccacctcggg cgagcggcca gcattgaggg tgccactgac
     3241 caggaactcg ggatcgggca gtgtgccact ctcgccgaag aggcgggcga agatccgcgg
     3301 ctggctgatg gtttcgggct ccgccccctg ctgccagacg gcgacgaacc ggccgtccga
     3361 aaaggccgcg atgtcgggcc gccgctcctg gcaggcggac ccggtgtcga gccggaggtt
     3421 ctccgccccg gcggccgcgt ggccggcggc aacggtgaga aggcaggtcg tggccagtac
     3481 cttatagatg gagcgcatgg atatcctcct gtctcaaaat gccgcggtgt cggcgcggct
     3541 gcgaagctgg ccggccgggt tgtggtacgt ctttacctca tcggtctcgg tgtcgcgcac
     3601 caggacggtg tactcgacgt cggtgagcgc gccgtagaac acccagaaat ggccgttgac
     3661 cgcccggcca tccagcacct tgaccaccag ctcgatgttg tccggatcga agaaccagag
     3721 gtagccgctg tcgctgctca ggctgacccc ctcggcgtcg ccggtgccgc cgttggcggt
     3781 ccaggccacc tccacctcga agcggccgtc gaggcagagc cccggccggc gggagtagcg
     3841 ctgcagctcc ggcggcgtgc agggaccttc ggtgggctct tcttcgccgg agtcgtcggg
     3901 aaagaggaaa cgcggcgccg tggaggcagt gccggcgccg gctgctgccg tcgcccggaa
     3961 ggccgcggtg tcggcgaagc tggcgagccg gccggccggg ttctcgtagg tcctggtctg
     4021 cccggtgacc ctgtccgtca cctccagggt gaaggcgacg tcggtgaggg aagcatagaa
     4081 gacccagaag ctgccgctga ctgcacggcc gtccagtacc ttcaccaaca gctcgacgtt
     4141 ctcctggccg aagaaccaga aagagccggc gtcgggggtg gcgtcggggg tgagctgcgc
     4201 tgcgtacccc ccgccggagc cgccggtgtg gccggtccag tggacccggg cctcaaaacg
     4261 gccgtcccgc agaaagagca cccccgggga cgtcgggatc tgccgggcat agagcccgct
     4321 gctgaagcag gtctccgcat cgcccggcag gagatcgccc gtctgccagg cgacgagggc
     4381 ctcaccgcgg gagcccagag tcagcgcggg cccctcgaag ctccaagtgt tccaggcatt
     4441 gaagaccggc tccaccagcg cgccgtcggg ctcgatgtgg gccaccgccc cgatggcgag
     4501 ggcggggccc gaggtgaggc gctgccagga cagcaggaag tcccctccag gggccgccag
     4561 gacctccggc gcggagacgc tctccacctc ccggccaccg aattcgagcc gcgggcccac
     4621 gggctgccgg tccgcggcaa agacctgggc tcgcagaacc gccccgactc ccgggctgcc
     4681 ctgctggttc caggccgcca ggacctggcc ggcggcgttg gcggccaccg cgggccgggg
     4741 ccgcggcaag tcgcccaggc cgtagggctc gaccacctcc aggacgggct ccgccggcgc
     4801 cgtgcccggg ggggcgatcc cgaagacgag ggtcctcgtc atctgtccct gatcctcgcg
     4861 cacccaggcc gccacaactc cggaagggcc atacggcgcc actgcggggt ccgcaaacag
     4921 ggaacgcgga ccggacggcc actcgagggc ccgggaggcg accgggggct gccccgtggg
     4981 accgatctgc cacaggatga ccagctcctc catcgtcgtg acgatggcaa agccgccgtc
     5041 gtgggtggcg gtcagc
//
LOCUS       DASPJI010000454         5122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_661250, whole genome shotgun sequence.
ACCESSION   DASPJI010000454 DASPJI010000000
VERSION     DASPJI010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661250"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /gene="dnaA"
                     /locus_tag="VEL74_12285"
     CDS             <1..1002
                     /gene="dnaA"
                     /locus_tag="VEL74_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HYG63353.1"
                     /translation="PRYTFDSFVSGPSNQLAFAASQAAGSAYPPKYNPVFICGGVGLG
                     KTHLLHAIGHEILRNRPHLRVLYLAAEQFVNELINSIRFDRMPEFRERYRTIDVLMID
                     DVQFIANKERTQEEFFHTFNTLYTSQKQIILSSDSSPRNIPTLEERLRSRFEWGLIAD
                     IQPPDLETKMAILQRKAASEGVQVPDEVAGFIAQQVRSNIRELEGLLTRVVAYGSLTG
                     KPVTLSLTRETLKDILPEEGRKPPATEIIKAVAHHYGLKLAEIKSKSNSRQVVIPRQV
                     AMYLCKKLTDLSYPEIGKLFNDKHHSTVMYSVEKVQQTRQSDPEFDRTLQAFEQQFS"
     gene            1326..2423
                     /gene="dnaN"
                     /locus_tag="VEL74_12290"
     CDS             1326..2423
                     /gene="dnaN"
                     /locus_tag="VEL74_12290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HYG63354.1"
                     /translation="MEIRVPRSEFVGELAPMQGIVERKTTIPILSHLLLTARGDKLHL
                     AATDLDVSLTSVCEADVRQEGGIAVQAKKLMEIVRSLGGDEVHLVQEEPRVLTIKAGR
                     SRFKIHGLPPDDFPTLPVVEDSQQVEIPFASFRRMVSKILFAVSAEESRFQLNGALLK
                     LKDGGLELVATDGHRLALVETSLSDGGGNDSVLVPRKALQELQRIEGDGSLAYRRGEH
                     HLSFRLGRRELICRILEGTFPDYERVIAKDNDKKVLFDRKPLSEAVQRVALLTGDRAR
                     AVRLQFGGEQLVITAANPDLGEAMEELACAYDGPEFRLGINPDYLIQFLGAVDTDRIR
                     LELKDENTQCVGYPVEGPDARYLCVIMPMRI"
     gene            2546..3613
                     /gene="recF"
                     /locus_tag="VEL74_12295"
     CDS             2546..3613
                     /gene="recF"
                     /locus_tag="VEL74_12295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HYG63355.1"
                     /translation="MLSQLTVQGFRNLEPLAWRPEAGSHLLLGGNGAGKTSLLESVYV
                     LATTRSFRAAQIADCVRHGTGSFHVRGEVETDRRATLEVGWVQGARSRSVNGKVTALA
                     EHLAVLPVVAWTAAEAQVVIGAPGARRRFLDRGVIGIRPPALEVLGRYREALRQKRQL
                     LARGESGVEIWNELLATAAFEVASLRHAYVERLREALSGVVAAAGLPFPPVEVRYRPS
                     PSRALEGVEAIADSLEKVADRERRRGTPLLGPQRDDLEILWGGHDIRRVASAGERKAL
                     SLLLLAAHGRVVEGAGRLPVYLLDDADAELAPATLHSLWGVFQGVRQLFASSNRPQVW
                     LTLEVGRVWEMERGRLIVLES"
     gene            3764..>5122
                     /locus_tag="VEL74_12300"
     CDS             3764..>5122
                     /locus_tag="VEL74_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011627759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HYG63356.1"
                     /translation="MNPESTYTAENIKVLKGLEAVRKRPGMYIGDTDDASGLHHMVFE
                     LVDNSIDEAQAGYCSRIDVTIHTDNSVTVEDDGRGIPVDIHKEEGRSAAEVIMTELHS
                     GGKFDKNSYKVSGGLHGVGVSVVNALSETMELEIWRDGQVWFQTYHQGVPDGPIKVIG
                     KTTRTGTKVRFWADPEIFAVLEFHYETLAQRLREQSFLNRGVHIHLKDERTDKEADFA
                     YAGGIASFVEYLNRNKTPLHPQPIYLFDRRDEGAELVEVALQWNDGYQELIYSFTNTI
                     NNRDGGAHLVGFKAALTRTVNAYAQASGLAKNLKENLTGEDIREGLTAIVSVKIKDPK
                     FSSQTKDKLVSSDVKGWVEQVVNDKLATFLEENPREARRIVEKCVEAAHAREAARKAR
                     DLTRRKGALDASNLPGKLADCSERNPELAEIFLVEGDSAGGSAKQGRDRKFQAILPLN
                     GKI"
BASE COUNT          877 a         1813 c         1653 g          779 t
ORIGIN      
        1 ccccgctaca cgttcgacag cttcgtctcc ggcccgtcca accagctcgc cttcgccgcc
       61 tcgcaggcgg ccggctcggc ctacccgccc aagtacaacc cggtcttcat ctgcggtggc
      121 gtcgggctgg gcaagaccca cctcctccac gccatcggcc acgagatcct gcgcaaccgg
      181 ccgcacctgc gggtcctcta cctggccgcc gagcagttcg tcaacgagct gatcaactcc
      241 atccgcttcg accgcatgcc cgagttccgc gagcgctacc gcacgatcga cgtcctgatg
      301 atcgacgacg tgcagttcat cgccaacaag gagcggacgc aggaggagtt cttccacacc
      361 ttcaacaccc tctacaccag ccagaagcag atcatcttgt cgtcggactc gtcgccgcgc
      421 aacattccca ccctggagga gcgcctgcgc agccgcttcg agtgggggct gatcgccgac
      481 atccagccgc cggacctgga gaccaagatg gccatcctcc agcgcaaggc ggcgagcgaa
      541 ggggtgcagg tgccggacga ggtggcgggc ttcatcgccc agcaggtgcg ctcgaacatc
      601 cgcgagctcg aaggcctcct gacccgcgtc gtcgcctacg gctcgctcac cggcaagccg
      661 gtcaccctct ccctcacccg ggagacgctc aaggacatcc tccctgaaga aggaaggaag
      721 ccgccggcga cagagatcat caaggcggtg gcccatcact acggcctcaa gctcgccgag
      781 atcaagagca agagcaactc gcgccaggtc gtcatcccgc gccaggtcgc catgtacctg
      841 tgcaagaaac tgacggacct ctcctacccg gagatcggca agctcttcaa cgacaagcac
      901 cactccaccg tcatgtactc ggtggagaag gtgcagcaga cccgccagtc ggaccccgag
      961 ttcgaccgca ccctgcaggc cttcgagcag cagttctcct gatcccctcc ggatcgcctc
     1021 ccgttccccc tctccggccc ggcgccggca cagggattgc ttacccgaaa aacgggggcc
     1081 atgccccgtg gggaaaactt gtggagcacc ttccggaagg cgctgtggag cgcctgcgga
     1141 ttttttccgc ccctccggtc ctccacagcc gctccgcaga ccttccacag cttccgcaca
     1201 gtacccttgc ggactcccaa agttccccct tacagtcact tagcccggtt ttcctcattt
     1261 ccgcaggcgc cttctacgaa gacggctatg ctatattcag gtctcttcta gtatggagaa
     1321 ggtgaatgga gatccgcgtc ccaaggagtg agttcgtcgg cgagctggca cccatgcagg
     1381 ggatcgtcga gcgcaaaacc accatcccga tcctctctca tctgctgctg acggcccgcg
     1441 gggacaagct ccacctcgcc gccaccgacc tcgacgtctc gctgacgtcg gtgtgcgagg
     1501 ccgacgtgcg ccaggagggg ggcatcgcag tccaggccaa gaagctgatg gagatcgtcc
     1561 gctcgctcgg cggcgacgag gtccacctcg tccaggagga gccgcgggtg ctcaccatca
     1621 aggccggccg ctcccgcttc aagatccacg gcctgccgcc ggacgacttc cccaccctgc
     1681 cggtggtgga ggacagccag caggtggaga tccccttcgc cagcttccgg cgcatggtgt
     1741 cgaagatcct cttcgccgtc tccgccgagg agtcgcgctt ccagttgaac ggcgccctct
     1801 tgaagctcaa ggacggcggc ctcgagctgg tggcgaccga cggccaccgc ctcgccctgg
     1861 tggagaccag cctctccgac ggcggcggca acgactcggt gctggtgccg cgcaaggcgc
     1921 tccaggagct gcagcgcatc gagggggacg gcagcctcgc ctaccggcgc ggcgagcacc
     1981 acctctcgtt ccggctcggc cgccgggagc tgatctgccg catcctcgag gggaccttcc
     2041 ccgactacga gcgggtgatc gccaaggaca acgacaagaa ggtgctcttc gaccgcaagc
     2101 cgctgtccga ggcggtgcag cgcgtcgccc tgctgaccgg cgaccgggcg cgcgccgtgc
     2161 gcctgcagtt cggcggcgag cagctcgtca tcacggcggc caaccccgac ctcggcgagg
     2221 cgatggaaga gctggcctgc gcctacgacg gcccggagtt ccggctcggg atcaaccccg
     2281 actacctgat ccagttcctc ggcgccgtcg acaccgaccg catccgtctc gagctcaagg
     2341 acgagaacac gcagtgcgtc ggctaccccg tcgaggggcc ggacgcccgc tacctctgcg
     2401 tcatcatgcc gatgaggatc tgagccccgc tgccgagacc cggcagtgag gtctgcccgg
     2461 cgcaggcggc tggcagcgtg gccgggaggg agggagagcg cacacgaggg agccgagcag
     2521 ggcagaccga acgacagggc cacttttgct ttcccagctc acagttcagg gattccgcaa
     2581 cctcgagccg ctggcctggc ggcccgaggc ggggtcccac ctgctcctcg gcggcaacgg
     2641 cgccggcaag accagcctcc tcgaatcggt ctacgtcctc gccaccaccc gcagcttccg
     2701 ggccgcccag atcgccgact gcgtgcgcca tggcaccggc tctttccacg tgcgcgggga
     2761 ggtagagacc gaccgccggg ccacgttgga ggtgggatgg gtgcaggggg cccgcagccg
     2821 ttcggtcaat ggcaaggtga cggcgctggc cgagcacctg gccgtgctgc cggtggtcgc
     2881 ctggaccgcc gccgaggccc aggtggtcat cggcgctccg ggggcccgcc ggcgcttcct
     2941 cgaccgcggc gtcatcggca tccgcccccc cgccctggag gttctcggcc gctaccgcga
     3001 ggcgctgcgc cagaagcggc agctcctcgc ccgtggggaa tccggagtgg agatctggaa
     3061 cgagctcctg gccaccgcgg ccttcgaggt ggcgagcctg cgccacgcct acgtcgagcg
     3121 gctgcgcgag gcgctctccg gggtggtcgc cgcggcgggg ctccccttcc cgccggtgga
     3181 ggtccgctat cggccctctc cctcccgggc cctggaagga gtggaggcga tcgccgacag
     3241 cctggagaag gtcgccgacc gggagcgccg ccgggggacg ccgctcctcg ggccgcagcg
     3301 cgacgacctg gagatcctct ggggcgggca cgacatccgg cgcgtcgcct cggccggcga
     3361 gaggaaggcg ctgtcgctgc tgctgctggc ggcgcacggc cgggtggtcg agggggcggg
     3421 gcgcctcccg gtctacctgc tggacgacgc cgacgccgag ctcgccccgg ccaccctgca
     3481 ctccctctgg ggggttttcc agggggtgcg gcagctcttc gccagctcca accggccgca
     3541 ggtctggctc accctggagg tcggccgcgt ctgggaaatg gaacgggggc gtttgattgt
     3601 tctagagagt tagaacggtt accctgcgct ggcgtaagcc gttgaatttt ctaggctcta
     3661 gccctcgctt ccggggtccc gggtgtggta tgatttacac acctcggctc ctcccgttcc
     3721 tcgaggggct cagcaatcag gcacagcgga gacaaatgtt ttggtgaatc cagagtccac
     3781 ctatacggct gagaacatca aggtcctcaa gggcctcgaa gcggtgcgca agcgcccggg
     3841 gatgtacatc ggcgacaccg acgacgcctc cggccttcac catatggttt tcgagctcgt
     3901 cgacaactcg atcgacgagg cgcaggccgg ctactgctcg cggatcgacg tcacgatcca
     3961 caccgacaat tcggtgaccg tcgaggacga cggccgcggc atcccggtcg acatccacaa
     4021 ggaggagggc cgctcggccg ccgaggtcat catgaccgag ctgcactcgg gcggcaagtt
     4081 cgacaagaac tcctacaagg tgtcgggcgg cctccatggt gtcggcgtgt cggtggtcaa
     4141 cgccctgtcg gagacgatgg agctggagat ctggcgcgat ggccaggtct ggttccagac
     4201 ctaccaccag ggggtgcccg acggtcccat caaggtgatc ggcaagacca cgcggaccgg
     4261 caccaaggta cgcttctggg ccgatcccga gatcttcgcc gtcctggagt tccactacga
     4321 gacgctggcc cagcgcctgc gcgagcagtc gttcctgaac cgcggcgtcc acatccacct
     4381 caaggacgag cgtacggaca aggaggcgga cttcgcctat gccggcggca tcgcctcctt
     4441 cgtcgagtac ctcaaccgga acaagacgcc gctccacccg cagccgatct atctcttcga
     4501 ccggcgcgac gagggcgcgg agctggtcga ggtggcgctg cagtggaacg acggctacca
     4561 ggagctgatc tacagcttca ccaacacgat caacaaccga gacggcggcg cgcacctggt
     4621 gggcttcaag gcggcgctga cccgcacggt caacgcctac gcccaggcct cggggctggc
     4681 gaagaacctc aaggagaacc tgaccggcga ggacatccgc gagggcctca cggccatcgt
     4741 gtcggtcaag atcaaggacc cgaagttctc gtcgcagacc aaggacaagc tggtctcctc
     4801 cgacgtcaag gggtgggtcg agcaggtggt caacgacaag ctcgccacct tcctggagga
     4861 gaacccgcgc gaggcgcggc gcatcgtcga gaagtgcgtc gaggcggcgc acgcccgcga
     4921 ggcggcgcgc aaggcgcgcg acctcacccg gcgcaagggc gcgctcgacg cctcgaacct
     4981 gcccggcaag ctcgccgact gctccgagcg caaccccgag ctcgccgaga tcttcctggt
     5041 cgagggcgac tccgccggcg gctcggccaa acagggccgc gaccggaagt tccaggcgat
     5101 cctgccgctc aatggcaaga tc
//
LOCUS       DASPJI010000455        14056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_323065, whole genome shotgun sequence.
ACCESSION   DASPJI010000455 DASPJI010000000
VERSION     DASPJI010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14056)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14056
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323065"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VEL74_12305"
     CDS             <1..1037
                     /locus_tag="VEL74_12305"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63357.1"
                     /translation="SHAAEAALAVRCGFPAERIAYARPVLTPEELDAALGLGVPLFHV
                     HHPDDLEKIEAAAARAGRPVRVSFRLRGAAGLSPLGRLNRRLGLDADEIVEAARRAVA
                     SPWLEPAALNFYLGTQQSSLDGFTRVFRGVLALLKRLASETGAGLHEVNLGGGIPSPT
                     LRRVGPRHVLGRLQDRLGASSSGNLETFASRLGERFRELVEASGLAAPPVLAAEPGRS
                     IVGDAAVLITRVRAVRGSWLFLDASRNHLPESPVLFARRILPLSEAGRGAERFWNLSG
                     CTLNTLDVLDLHRRLPPLTPGQGLAFCDAGAYSISRASPYAGLAPAVHLLEADGTVRL
                     VRRAEGVEDL"
     gene            1034..2002
                     /locus_tag="VEL74_12310"
     CDS             1034..2002
                     /locus_tag="VEL74_12310"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYG63358.1"
                     /translation="MSALVTGASGFLGRHLVRTLLDAGRQVVAHTRDPGALSDLRHPA
                     LQVTSGDLRDPASYSPWLVPGASVFHLAAARNDPRVPRVRAREMEEVNVGATAELARR
                     SLEAGVGRFVHVATALIHGPSDGEGHPFGAYMESKARAVREVRRLAGEGLRAVIVCPS
                     IVYGPDHPSHPNRVTSEVRRLLRRRVVRVVGGGRQARNLVYVDDVVRGLLAAERQGTV
                     GEEYVLGGEEIAPRDFGRLVLSLAGLRPLATLSLPAAPALAMARIADRIRKMEAGSGY
                     TTAVQVLLREWRVSSDRAARDLEHHPVSLAGGLAQTLRWMGESGSA"
     gene            1995..2822
                     /locus_tag="VEL74_12315"
     CDS             1995..2822
                     /locus_tag="VEL74_12315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG63359.1"
                     /translation="MHSSSWDEATRDLRERQRRDWDRIAASVRDFHAAPSTQYYRRCE
                     MALLERSVGSLRGKRVLKLDLWNEAFNTRILDWMREQGAEVVGLDISRVVAFRARRNS
                     LSNGARSNGHAPALLRADIRELPFADGSFDIVYTMGTIEHIDEYRQAIGEVQRVLKPG
                     GRAVVGVPHKWDLFLRPLMVWILDAFGQYLYAPEKSFSASELRQDVERTGLRVVERTG
                     ILTLPGFLRMADLFFYTRGLPFYRLSPLFLAPFQFAETRWRWPGRFGYLLTVVADKP"
     gene            2835..3470
                     /locus_tag="VEL74_12320"
     CDS             2835..3470
                     /locus_tag="VEL74_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63360.1"
                     /translation="MELYRHVPGFLSAEEVSRFLRLLEDHEDLYTEVHGRGGLGPRYR
                     VIDGDQIHEMLPEVEALGDRRVRPLAEEFAGQPLRPLGSSKRSMRVQTYERREHGFRW
                     HLDGHAYVVLVTLQNTNGGQTQFISPGLTRFLRFLLYPLYAFPQVFSIFPRRAVTAHA
                     GDLLAMRGARAIHRGVTLSDQGERILIAYTFDAPDKKPNPFRDWIAKRLNY"
     gene            3467..5209
                     /locus_tag="VEL74_12325"
     CDS             3467..5209
                     /locus_tag="VEL74_12325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYG63361.1"
                     /translation="MSAPALVSFLAAHLLTLAVLTATAWAAGSWVRLPMEGRAERLAI
                     PIAAGLAVLAHLGFLLASLGLLRPGILIALIVAIHCAGFRRWRSAFADLPDRSGRTIA
                     LGLVALVPVFILALYPPAAFDETMYHLPFARAFALSGGLPATPALRFPVFPALAELLL
                     AEMLLLAGDVATHLVAFVAVLATAGLLVAWGRRVFSATAGWIAAALYLGNPIVVSLAG
                     TDYVEPLLTLFVTGALFAAWSYRISGERAWLLLAAGLAGSAAGVKYLGLFFAAAIFTD
                     ILIDRRKLSAPLLFAALSFVVLAPTYARIMIHTGNPIFPYLPGIFGSTPWDPIALKPL
                     REMMSSETLIRFIRLPWDVVFHRSAVGEQPPFSPFYLLGLPLLLAGAAREPRVRRLVV
                     MPLVWGAVFLFLPRDSRYLVPILPPLSLALAGLIKIEKRSLVAAVCALAFLPGWLYAG
                     YRIGRQGPLPVSEEERDLYLARKLPLYPAVRHLDRISSVAYAFEAENMKYFYDGDLHG
                     DWIGRASYRRLFPLVETPEALHRELRELGASHLLVSKRAIQPRKGADWPRWFRKVYED
                     PAAEVFALAGGSER"
     gene            complement(5198..7273)
                     /locus_tag="VEL74_12330"
     CDS             complement(5198..7273)
                     /locus_tag="VEL74_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HYG63362.1"
                     /translation="MAPLTRRELILAAAAMGATWACAGSRALRSRSPWIERRDRFAEG
                     VASGDPGPDSVLLWTRASDRGTEPAVPLTVEVAEDPGFERVVATAPTRALLEADHTCR
                     VLVGGLRPGRTYWYRFIDEKGDGSRIGRTRTAPADDDPRPVRFAFVSCQNICEGAQNA
                     YRRMIFEDERAAPEDQLAFVLHLGDFVYEVVDYPEEVPGGHRYDRRLRDAVRFPDGEK
                     VSGFWVPASLADYRVLYRAYLRDPDLQDARARLPFVAMWDNHEFSWMGWQSFQRFEGK
                     ARSAQAIKVAANQAWFEYQPARVSHAGGPSLERFDAPPVQNAPIQRFDEQGLGQEPNN
                     LAAIGSLTAYRTLRWGRFLELLITDQHSYRSEVPDGREEARPLQSGDFPELVPLEMMQ
                     ALDAGRAYADGRPPETIRYGEVQVPNFRKDEAPQTILGAEQKAWFLDRLRASRATWKV
                     WGNSLGSLDWRADPQNLPAGLGTPWPGNGYACFGGGGDWSTAYTERGQIYDTVRDAGI
                     TGFLTVSGDRHSFWAGLAAKALPPAPFEPVGAAFITGSISAPGLVEAFEHRFPQDHPL
                     RALYMTEVGGRKQPMVNLLMHHGVRACLEYQRSGDAEQARRASNPDLAPHLSFLDMGG
                     HGYATLHLTADAAECELVCIPRPLERSEGPDGGPLRYRVVHRVPMWNAGERPRLERRV
                     VEGDPGLSL"
     gene            complement(7330..9585)
                     /locus_tag="VEL74_12335"
     CDS             complement(7330..9585)
                     /locus_tag="VEL74_12335"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYG63363.1"
                     /translation="MNYLDLTLRVDTGDGNGLVVRLVESPAGQAEAPFVLPLIEEEVQ
                     QAAIGFERCLDEEESYRDMHSRAADDESRQRAIGDQLFQALFPEPIRDLYYQSLGLLG
                     SRPDLGLRIKLQMGLGDPRLTAVHGLPWECLYRAKDGTFLGRERRSSIARHLDLPLPG
                     GRPSLKGPLRVLVVAAQPRGLRRLPHLERERHALLQLGRRQEGMEMIPLATPTLKALR
                     ETLLAGDFHVLHFLGHGGFEQTTGAGLLYLEDEAGGPAPVKGPALADLLRGVASLRLV
                     VLNACQTAKLAAPGPFGGLATALLRAGLPSVVAMQFPISDRAANSFSTAFYRRLSLGD
                     PIDAAVTEGRLAIRSDLPDSAEWATPALFLRAPDGKLFEPRRPVRKRWTAAAMVVTLC
                     LAAGLLLLQSPEGSSSRDRPDTVITADAPQSVEPGAATKSHEVRWMTNIETLVRVLGS
                     AWGVWSLVAFALGIFFAIMSTVIRKARPGDFNGEQTYRFFRLAMVLAFLLALATLITA
                     TTKQVMAKPGVAKEERIQSLTLDLKSDDPTQRRQAIENLVPYGGDAAREVVKAVGEEA
                     GVIAAEMMGRATGGDWIDILSGFLGAPPWQTPFMVSAMDCLIEIGQPSIPFILGQLST
                     DSVEAERLMAEAAQRQAPAQNPNALEAFGDWGFLLGAAGQGMRIGMTRDILSRVLLAI
                     GPPAVPELLDGLESPRLLVQMTSYQVLEQIPSATEATIQRLRRIAARSSDPAERQQIL
                     AAIQQLEARRP"
     gene            9712..10620
                     /locus_tag="VEL74_12340"
     CDS             9712..10620
                     /locus_tag="VEL74_12340"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYG63364.1"
                     /translation="MPKKSAIGRTVVLSSLRAGLAAASTLSPQLAGAGAARLFLSTRR
                     HPMPEWERAQLASARRSRLASEWGPLAVWTWGPEDAPAMVLQHGWEGRGSQLGAFVPP
                     LLAAGYRVVALDGPGHGDSPGRSSSLVALGQALRLLGGSLGPLAGVVAHSAGTVAAIH
                     ALSRGLVADRLVAIAPGVDLEAYAREFARMFGLSAAVSRSMRQRIERRIGVSWEELDP
                     RRAPAHVRIPLLVIHDRTDREAPYAGGEALARAWGARLITTEGLGHTRILRNEQVVAQ
                     TTEFLSVPKATAEVLVLATEYASAAR"
     gene            complement(11086..11544)
                     /locus_tag="VEL74_12345"
     CDS             complement(11086..11544)
                     /locus_tag="VEL74_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63365.1"
                     /translation="MAERDEPPTAGEADVAGPWRIEEVLGEGFGLFWIGESLVRGFSP
                     VAAFPSRWLALIAAALLPGTGRDPLLSIDKQPDADGAYPLLLPGLGGEEVAGRLRVFD
                     ETLVDSMNVAIHLLQAPESLAWMLEAAEAVALERCGAILDERTAASGAAG"
     gene            complement(11980..12801)
                     /locus_tag="VEL74_12350"
     CDS             complement(11980..12801)
                     /locus_tag="VEL74_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63366.1"
                     /translation="MRNRRILVGFVSSLLLSLSPLAAAPVIERGIDVFTTAADGKTSY
                     DFARNPIPAGFFCKSSKPFSGRIQLKGLPLATEIAGQLEGADTVVERLDDAVFDDYGR
                     AMTRIQFRALSLVSIAPLKTSCGAFHTYISLSGKQRVTTMNLQRTQAGGGSFTVPVAV
                     DIRMTFIPVKPARGQRARNLELTGSFIFPARPIPWSFVDSAMTKSSGPVVVDTNGDLA
                     PDAWLPGTSNFLPGRSPGSRNKATYCCPEEVCHYESPGCAHCTVTYPAGCQGHCP"
     gene            13016..13867
                     /locus_tag="VEL74_12355"
     CDS             13016..13867
                     /locus_tag="VEL74_12355"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015843525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYG63367.1"
                     /translation="MEIQPLLTDLVERVKRVQGVRAIVLGGSRARGTHTPKSDIDLGI
                     YYHPDQPLDLQALDQASAELDDRHQPGLLTPIGGWGPWINGGGWLTVQGQPVDFLYRD
                     LGKVSACIEACCAGEVEMVYQPGHPHGFSSSIYMAEVAACQILWEADGQLSALKERTD
                     PYPEPLKLALIQKFGWEMGFSLMIARKAIERGDVAYVAGCCFRCVGCLLQVVFALNGQ
                     YWMNEKGAVALADTFLVRPAMLRGRIEKAFTLLAADRLALTEAIAVLDEVKRDTDLLA
                     TPRTDPA"
     gene            complement(13842..>14056)
                     /locus_tag="VEL74_12360"
     CDS             complement(13842..>14056)
                     /locus_tag="VEL74_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HYG63368.1"
                     /translation="AMLGGRIKPENCRKPEIVADAAYLILTKDSRSTTGNFFIDEDVL
                     AGAGITDLDRYAVQPGAPLMPDLFLE"
BASE COUNT         2140 a         4702 c         4788 g         2426 t
ORIGIN      
        1 cgagccacgc ggccgaagcg gctctcgcgg tccgctgcgg cttcccggcg gagcggatcg
       61 cctacgcccg ccccgtgctc acgccggaag agctggacgc ggcgctcggc ctcggggtgc
      121 cgctcttcca tgtccaccat ccggatgacc tggagaagat cgaagcggcg gcggcgcggg
      181 ccggccgtcc ggtccgagtc tcattccggc tgcgcggcgc ggcgggcctc tcgcccctcg
      241 ggcggctcaa ccggcgtctc gggctcgacg cggacgagat cgtcgaggcc gccaggcggg
      301 ccgtggcgtc tccctggctg gagccggccg ccttgaactt ctatctcggc acccagcagt
      361 cgtcgctcga cggcttcacc cgcgtgttcc gaggcgtcct ggccctcctg aagcgactgg
      421 cctcggagac cggggcgggg ctccacgagg tgaacctcgg cggtggcatc ccgtcgccca
      481 cgctgcggcg ggtagggccg cgccatgtgc tcgggcgtct gcaggaccgg ctcggcgcgt
      541 cctcctccgg aaacctggag acgttcgcct cccggctggg ggagcggttc cgcgagctgg
      601 tcgaggcctc cggtctcgcc gcgccgccgg tcctcgcggc cgagccggga cggtcgatcg
      661 tcggcgacgc cgcggtgctg atcacccgcg tccgcgccgt ccgggggagc tggctcttcc
      721 tggacgcgag ccgcaatcac ctgcccgaat cgcccgtgct cttcgcccgg cgcatccttc
      781 cactctccga ggctggaagg ggagccgagc gcttctggaa cctgtccggc tgcaccctga
      841 acacgctcga cgtgctcgac ctgcaccgcc gcctgccgcc gctcacgccc ggccagggtc
      901 tcgccttttg cgacgcggga gcctattcga tctcccgcgc gagcccctat gcggggctcg
      961 cgcccgcggt ccacctcctt gaagcggatg gaacggtccg gttggtccgg cgggccgagg
     1021 gcgtggagga tctgtgagcg ccctggtcac gggagcctcg gggtttctgg gacgccacct
     1081 ggtcaggacc ctgctggacg cgggccgtca ggtcgtggcg cacacccgcg atccgggggc
     1141 gctctccgat ctgcgccatc cggctctcca ggtcacctcc ggcgacctcc gcgatccggc
     1201 ttcgtactct ccgtggctcg tgcccggggc ttcggtgttc catctcgccg ccgcgcgcaa
     1261 cgatccccgg gttccccgcg tccgtgcccg cgagatggag gaggtcaacg tcggcgcgac
     1321 ggccgagctc gcccggcgtt ccctggaggc cggggtgggg cggttcgtcc atgtcgcgac
     1381 ggccctgatc catggtcctt cggacggcga gggtcatccg ttcggcgcct acatggagtc
     1441 caaggcacgc gccgtccggg aggtccggcg gctcgccggc gaggggctgc gcgcggtcat
     1501 cgtctgcccg tcgatcgtgt acggcccgga tcacccgtcc caccccaacc gggtcacctc
     1561 cgaggtccgc cgcctcttgc gccggcgggt ggtgcgggtc gtgggcggcg gccggcaggc
     1621 gcgcaacctg gtctacgtgg acgacgtggt gcgcggcctc ctggccgccg agcgccaggg
     1681 caccgtgggc gaggagtacg tgctgggagg cgaggagatc gccccccggg attttggccg
     1741 tctcgtcctc tcgctcgccg gtctccggcc gctcgccacg ctctcgctgc ccgccgcccc
     1801 ggcgctcgcc atggcgcgga tcgccgaccg gatccggaaa atggaggctg gaagcggtta
     1861 cactaccgcg gtccaggtgc tcctgcgcga gtggcgtgtc agctctgacc gagcggcgcg
     1921 ggacctggag catcatcctg tctcgctggc gggggggctg gcgcagacgc tcagatggat
     1981 gggggaatca ggtagtgcat agctcgagtt gggatgaagc gactcgggac ctgcgcgagc
     2041 gccagcgccg ggactgggat cggatcgccg cgtcggtgcg cgacttccac gccgcaccga
     2101 gcactcaata ttaccggcgc tgcgagatgg cgctgctgga gcggtcggtg ggcagcctgc
     2161 ggggcaagcg ggtgctcaag ctggatctct ggaacgaggc cttcaacacg cggatcctcg
     2221 actggatgcg cgagcagggg gcggaggtgg tggggctcga catctcccgc gtcgtggcct
     2281 tccgggcgcg ccgcaattcg ctctcgaacg gcgcccgctc gaacggtcac gcgccggccc
     2341 tgctgcgcgc ggacatccgc gagctgccgt tcgcggacgg ctccttcgac atcgtctata
     2401 cgatgggcac catcgagcac atcgacgagt accggcaggc gatcggcgag gtccagcgcg
     2461 tgctcaagcc gggtggccgt gcggtggtcg gcgtgccgca caagtgggac ctcttcctgc
     2521 gcccgctcat ggtctggatc ctggacgcct tcggccagta cctctacgcg ccggagaagt
     2581 cgttcagcgc cagcgagctg cgccaggacg tcgagcggac cggcctgcgc gtggtcgaac
     2641 gcacggggat cctcaccctg ccggggttcc tgcggatggc cgacctcttc ttctataccc
     2701 gcggcctgcc gttctaccgg ctgagccctc ttttcctcgc ccccttccag ttcgcggaga
     2761 cgcgctggcg ctggccgggc cgtttcgggt acctgctgac ggttgtcgcc gacaagccct
     2821 aggcggagga gcgcatggaa ctgtaccgtc acgtgccggg gttcctctcc gccgaggagg
     2881 tgagccggtt cctccgtctg ctcgaggacc acgaggacct gtacaccgag gtgcacggcc
     2941 ggggtggcct gggcccgcgc taccgcgtca tcgacggcga ccagatccat gagatgttgc
     3001 ccgaggtgga ggcgctcggc gaccggcgag tccggccgct ggccgaggag ttcgcgggcc
     3061 agcctttgcg gcccctcggc agctccaagc gttccatgcg cgtccaaacc tatgagcgga
     3121 gggagcacgg cttccgctgg cacctggacg gacacgccta cgtggtcctg gtgacgctcc
     3181 agaataccaa cggcgggcag acgcagttca tctcccccgg cctcacgcgc ttcctgcgtt
     3241 tcctgcttta tcccctctac gcgttcccgc aggtcttctc gattttcccg cgccgcgccg
     3301 tcaccgctca cgcgggggac ctgctggcga tgcgcggcgc ccgcgccatt caccgcggcg
     3361 tcaccctgtc ggatcaaggg gagcgcatcc tgatcgccta caccttcgac gcgccggaca
     3421 agaagccgaa tccgttccgg gattggatcg ccaaaaggct gaattattga gtgcgccggc
     3481 cctcgtctcc ttcctcgcgg cgcacctgct gaccctggcc gtcctgaccg ccaccgcctg
     3541 ggcggccggc tcctgggtca ggctgcctat ggaaggccgg gcggagcggc tcgcgattcc
     3601 gatcgcggcc gggctcgcgg tgctggccca cctcggattt cttctcgcct cgctcggcct
     3661 gctgcggccc gggattctga tcgctttgat cgtggcgatt cattgcgccg gcttcaggcg
     3721 atggcgttcg gctttcgcgg atctccccga tcgatccgga cggacgatag ccctcggtct
     3781 cgtcgcgctc gtgccggttt tcatcctggc gctctatccg ccggccgctt tcgacgaaac
     3841 catgtaccat ctgcccttcg cccgcgcctt cgcgctgtcg ggagggctgc ccgccacacc
     3901 ggcgctccgg ttcccggtgt tcccggcgct cgcggagctg ctcctcgcgg agatgttgtt
     3961 gctggccgga gacgtggcga ctcatctcgt cgccttcgtc gcggtgctgg cgacggccgg
     4021 cctgctggtg gcctggggcc ggagggtctt ctcagccacg gccggctgga tcgcggcggc
     4081 gctctacctg ggcaatccga tcgtcgtctc gctggcgggc acggactacg tcgagccgct
     4141 cctgacgctc ttcgtcaccg gcgcgctctt cgcggcctgg agctatcgaa tttcgggtga
     4201 acgggcatgg ctgctgctcg ccgccgggct cgccggttcg gcggcgggcg tcaagtacct
     4261 ggggctcttc ttcgcggccg ccatcttcac cgatatcctg atcgatcgcc ggaagctgtc
     4321 cgcgccgttg ctgttcgcgg ctctgtcctt cgtggtgctc gcccccacgt acgcgcggat
     4381 catgatccat acggggaatc cgatcttccc gtatctcccc ggcatcttcg gatcgactcc
     4441 ctgggatccg atcgcgttga aacccttgcg agagatgatg tcctccgaga ccctgatccg
     4501 gttcatccgt ctcccctggg acgttgtctt ccatcgctcg gccgtggggg agcagccgcc
     4561 gttctcgccg ttctatctcc tgggccttcc cctgctcctc gccggcgccg cgagggagcc
     4621 ccgggtgcgc cgcctcgtcg tgatgcccct cgtctgggga gccgtcttcc tcttccttcc
     4681 ccgggactcc cgttacctgg tccccatcct gccgccgctg agcctggcac tcgccggatt
     4741 gatcaagatc gagaagcggt ccctagtagc ggcggtctgc gcactggcct tcctgccggg
     4801 ttggctctat gccggctacc gcatcggacg gcagggaccg ctgccggtca gcgaggagga
     4861 gagggacctc tatcttgccc gcaagctgcc gctctatccg gcggtccgtc atctcgaccg
     4921 gatctcctcc gtcgcctacg ctttcgaggc cgagaacatg aagtatttct atgacgggga
     4981 cctgcacggc gactggatcg gcagggccag ctacagacga ttgttccctc tcgtcgagac
     5041 tccggaggcc ctccatcggg agctgcggga gctcggagcc agccacctcc tggtgagcaa
     5101 gcgagcgatc cagccacgca agggggcgga ttggccgcgc tggttcagga aggtttacga
     5161 agatccggcc gcggaggtgt tcgcgctggc cggaggctca gagcgatagc ccgggatctc
     5221 cttcgacgac gcgccgctcg aggcgtggcc gctcacccgc gttccacatc ggcacgcgat
     5281 gaacgacgcg atagcggagc gggccgccgt ccgggccctc gctgcgctcg agcggccggg
     5341 gaatgcagac gagctcgcat tcggccgcgt ccgccgtcag gtgcagcgtg gcatagccgt
     5401 gaccgcccat gtccaggaaa gaaaggtgcg gcgccaggtc cgggttcgag gcgcggcgcg
     5461 cctgctcggc atcgccgctg cgctgatact ccaggcaggc ccgcacgccg tgatgcatca
     5521 gcaggttcac catcggctgc ttcctgccgc cgacctcggt catataaagg gcgcgcagcg
     5581 ggtggtcctg ggggaatcgg tgctcgaagg cctccaccag gccgggcgcg gagatggagc
     5641 cggtgatgaa cgcggcgccg accggctcga agggagcggg cggcagggcc ttcgcggcaa
     5701 ggcccgccca gaagctgtgc cggtcgcccg atacggtcaa gaatccggtg atgcccgcgt
     5761 cgcgcacagt gtcgtagatc tggccgcgct ccgtgtaggc cgtgctccag tcgccaccgc
     5821 cgccgaagca ggcgtagccg ttgcccggcc agggcgtgcc caggccggct gggagattct
     5881 gcggatcggc ccgccagtcc aggctcccca gggagttgcc ccataccttc caggtggcgc
     5941 gcgaggcgcg cagccggtcg aggaaccacg ccttctgctc agcgccgagg atggtctggg
     6001 gcgcctcatc cttccggaag ttcggcacct ggacctcgcc gtagcggatc gtctccggcg
     6061 gccgcccgtc cgcgtaggcc cgcccggcgt cgagggcctg catcatctcc aacgggacga
     6121 gctccggaaa gtcgccgctc tggagaggcc gcgcctcctc ccggccgtcc ggcacctcgg
     6181 agcgatagct gtgctgatcg gtgatcagaa gctccaggaa tcgtccccag cgcagggtgc
     6241 ggtacgccgt gaggctgccg atcgcggcga ggttgttcgg ctcctgtccg agcccctgct
     6301 cgtcgaaccg ctggatcggc gcgttctgca ccggcggcgc atcgaagcgc tcgagcgacg
     6361 gcccgcccgc gtggctcacg cgcgccggct gatattcgaa ccacgcctga ttggccgcca
     6421 ccttgatcgc ctgggccgaa cgcgccttgc cttcgaagcg ctggaacgac tgccagccca
     6481 tccacgagaa ctcgtggttg tcccacatcg cgacgaaggg gagccgcgcc cgcgcgtcct
     6541 gcaggtccgg atcgcggaga taggcgcggt agagcacgcg atagtcggcg aggctcgccg
     6601 gcacccagaa ccccgagacc ttctcgccgt cggggaagcg gacggcgtcg cgcagccggc
     6661 ggtcgtagcg gtgcccgccg gggacctcct cggggtagtc cacaacctca tagacgaaat
     6721 cgccgaggtg gagcacgaac gcgagctggt cctcgggagc ggcccgctcg tcctcgaaga
     6781 tcatgcgccg gtaggcgttc tgggccccct cgcagatgtt ctggcaactg acgaacgcga
     6841 agcggaccgg ccgcgggtcg tcgtcggccg gcgcggtgcg ggtgcgtccg atccggctgc
     6901 cgtcaccctt ctcgtcgatg aaccggtacc agtacgtgcg ccccggccgc agcccgccca
     6961 cgaggacgcg gcaggtgtgg tcggcctcga ggagggcgcg ggtgggggcc gtggcgacca
     7021 cgcgctcgaa gcccgggtcc tcggcgacct ccacggtcag ggggacagcc ggctccgtcc
     7081 ccctgtcgga ggcgcgcgtc cacagcagga cgctgtcagg accgggatcg cccgaggcta
     7141 ccccttcggc gaaccggtcg cggcgctcga tccagggcga gcgcgagcgc agagcgcgag
     7201 accccgcgca ggcccacgtc gcccccatgg cggccgcggc gaggatcagc tccctgcgtg
     7261 tcagaggtgc catggctgcc gagtgtagcg ggcaggcggc gggtcttgct agtgctctgc
     7321 caccgtccct cagggacggc gagcttcgag ctgctgaatc gcagccagaa tctgctgacg
     7381 ctcagcaggg tcgctcgagc gcgcggcgat tcggcggagc ctttggatgg tggcctcagt
     7441 cgcgctcgga atctgctcca agacctgata gctggtcatc tggaccagga gccgcgggct
     7501 ctcgagtcca tcgagcagct ctggcacggc aggcgggccg attgccagga gcactctcga
     7561 caagatgtct cgagtcatcc ctattctcat tccctggccc gcggcaccaa gcagaaaacc
     7621 ccaatcgcca aaagcctcca gcgcatttgg gttctgggcc ggagcttgac gctgtgcggc
     7681 ttctgccata aggcgctctg cttccactga atcggtggag agctggccga gaataaaagg
     7741 gatcgaaggc tgaccaatct cgatgagaca atccattgca gagaccatga acggcgtctg
     7801 ccacggtggc gctcccagaa agccggagag gatatctatc caatcgcctc cagtcgctcg
     7861 ccccatcatt tcggcagcta tgacacccgc ctcttcgccg acagctttga ccacttcgcg
     7921 cgctgcatcg ccaccgtaag gaaccagatt ctcgatggct tgtcgtcgtt gagtgggatc
     7981 atcgctcttg agatcgagag tcagactttg aatgcgctct tctttcgcta cccccggctt
     8041 cgccataact tgtttggtgg tggccgtgat cagggtggcc aaggccagga gaaaggccag
     8101 caccatggcg agcctgaaaa agcggtaggt ctgttcgccg ttgaaatccc ccggcctcgc
     8161 tttccggatg acggtggaca tgatggcaaa gaagattccc aaagcaaagg cgacgaggct
     8221 ccaaacaccc caggcactgc cgagcactct cacgagagtt tcaatattcg tcatccacct
     8281 cacctcgtgg ctcttggttg cagcccccgg ctccacgctc tgtggagcat ccgccgtgat
     8341 aaccgtatcc gggcgatcgc gagagctcga gccctccggg ctctgaagca gcagcaggcc
     8401 cgccgcgagg cagagggtga ctaccatggc ggcggcggtc catcgtttcc tgaccggccg
     8461 gcgcggttcg aagagctttc cgtccggggc acgcagaaag agggccggcg tcgcccactc
     8521 ggccgaatcg gggaggtcgc tccggatcgc caggcgcccc tcagttacgg cagcatcgat
     8581 agggtcgccc aaggagaggc ggcgatagaa cgcagtacta aaggagttgg ccgcgcggtc
     8641 cgagatgggg aactgcattg cgaccaccga cgggaggcct gcgcggagca gagccgtggc
     8701 aaggccgccg aacgggccgg gagccgccaa tttcgccgtc tgacaggcgt tcagaacgac
     8761 gagacgaagc gaggcaactc cccggaggag atcggcaagc gctggcccct ttaccggtgc
     8821 cggccctccc gcctcgtcct ccagataaag cagtcccgcc ccggtagtct gctcgaatcc
     8881 gccatgccca aggaaatgga ggacgtggaa atcgccggcg agaagcgtct cgcgcagagc
     8941 tttcagcgtg ggcgtggcga ggggaatcat ctccatgccc tcctgccggc gtcccagttg
     9001 gagtagggcg tgtcgctccc gctccaggtg aggaagtctt ctgagcccac gcggctgggc
     9061 ggcgaccacg agcacacgta gcggcccctt gagcgaaggt ctccctccgg gtagcggcag
     9121 gtccagatgg cgggcgatcg atgatctgcg ctcgcgtccg aggaatgtcc catccttggc
     9181 ccggtacaga cactcccagg gcaagccgtg gacggcagtc agccgcggat caccgaggcc
     9241 catctgcagc ttgatcctca aacccaagtc tggacgcgac ccgagaagcc ccaggctctg
     9301 atagtagagg tcccggatcg gctcgggaaa gagtgcttgg aagagttggt ctccgatcgc
     9361 ccgctggcgg ctctcgtcgt cggccgctcg cgaatgcatg tctcgatagc tctcttcctc
     9421 gtccaggcac cgttcaaaac cgatcgcggc ctgctggacc tcctcctcga ttaggggcaa
     9481 cacgaacgga gcctcggcct gcccggcagg cgactctacc agacgcacga cgaggccgtt
     9541 cccgtctccg gtgtcgacac gcagtgtcag gtcgagatag ttcatgctgg atcgctagag
     9601 tctatgtcat gagcaccgcg agtgatcgcg aatcagagag ggaacccccg cttgttgaca
     9661 aagaaacgaa cgatcgtgct attattgtcg tcgcagagag gagagctgac gatgcccaag
     9721 aagagcgcga tcggccgtac ggtagtcctg agcagcttgc gggcgggtct cgccgccgcg
     9781 agcacgctgt ccccgcagct cgccggcgcc ggcgccgcgc gcctgttcct gagcacgcgg
     9841 cggcatccca tgccggagtg ggagcgggcg cagctcgcga gcgcccgtcg ttcgcggctc
     9901 gcgagcgagt gggggccgct cgcggtctgg acctggggtc ccgaggacgc tccggccatg
     9961 gtcctgcagc acggctggga ggggagggga tcgcagctcg gcgccttcgt gccgccgctg
    10021 ctcgccgcgg gctatcgggt ggtcgctctc gacgggccgg ggcacggcga ctcgccgggc
    10081 cgcagctcct cgctcgtcgc cctggggcag gcattgcggc tgctcggcgg ttccctcggt
    10141 ccgctcgccg gggtcgtggc gcactctgcc ggcaccgtgg ccgcgatcca cgccctgtcg
    10201 cgcgggctcg tagcggaccg gctggtggcg atcgcgccgg gcgtcgacct cgaagcctac
    10261 gcgcgcgagt tcgcccggat gttcgggctg tcggccgcgg tgagccgcag catgcggcag
    10321 cgcatcgagc gccgcatcgg agtgagctgg gaggagctcg atccgcgccg ggcgcccgcg
    10381 cacgtgcgca tcccgctcct cgtcatccac gaccgcaccg accgggaagc gccctacgcc
    10441 ggcggcgagg ccctcgcccg cgcctgggga gcccggctga tcaccaccga ggggctcggg
    10501 cacacgcgca tcctgcggaa tgagcaggtc gtggcgcaga cgacggagtt tttgtcggtg
    10561 ccgaaggcga cggccgaggt cttggtattg gcgacggagt acgcttctgc cgccaggtag
    10621 tgccgccaga gggtcgagga taagcttatc tcggctccgc tgagcttagt ggggacgacc
    10681 taagcttagt gggcgcgttt aggattagcc aggacgactt aggcttagat ccggctcgcc
    10741 aagctcatag gagcctcgcc aagcctatct ggaacgacct gagcctatct ggaatacgct
    10801 aagcttatcc ggaacagact aagcttgtct gcatcgagct ggcgttagca gagccggctc
    10861 aagcttagtt gtgccggggt aggcttgtct ccgtcccgcc aagcctcgtt ggaccccgcc
    10921 aagtttagcg ggagcgggct aaggttagcg ggagcgggct gaggttagct gcggcgagtt
    10981 gacgttagca ggaccgactt gagcttgtgg atccggggga aagataggat gggggcgtca
    11041 cgctacggga acgcccccac ccggctctat ctataaggaa gggatctacc cggcggcgcc
    11101 gctcgccgcc gtccgctcgt ccagaatcgc gccgcagcgc tcgagggcga ccgcctccgc
    11161 cgcctccagc atccaggcga gggactccgg cgcttggagc aggtgaatgg cgacgttcat
    11221 ggagtcgacg agggtctcgt cgaacacccg gagccgtccg gcgacctcct cacccccgag
    11281 gccggggagg aggaggggat aggcgccgtc cgcatcgggc tgcttgtcga tgctgagcag
    11341 cgggtcccgc cccgtgccgg gcagcaaggc ggcggcaatg agggccagcc accgggacgg
    11401 gaacgccgcc acgggggaga acccccgcac caggctctcg ccgatccaga acaggccgaa
    11461 gccctcgccc aggacctcct cgatccgcca aggcccggcg acatcggcct cgccggccgt
    11521 gggcggctcg tcccgctcgg ccatccggca aagaaactcc acagtaaaga tgttggggtg
    11581 cacaccagag ctgataggct gctcgtagcc tcgcatagtt cctccttgag aggatcttcg
    11641 tggggttagg cctgggggag agctcgaact ctcttccggg ccgtaagcca gcaccatgcc
    11701 ggccgcccct ccttccagag atgttggctg gattgactga accgctcttc cgtttcccct
    11761 cgacaccccc ttctcccggg aagatagcgc gccgggttgc tgcgggagag gagtatatag
    11821 ggcggtgaag gtggaagcaa gagggttggc gaagattgtg gttaatcttc gcgtcacccc
    11881 ctaagactgg taggcctgag ggagccctag tgaccgaagg atcctacagg ggcaggaagt
    11941 ggcctggtca ccagggcctg aagaccagag aattaagatc tatgggcagt gtccctgaca
    12001 gcccgcgggg tacgtaaccg tgcagtgtgc acagccggga gactcataat ggcagacctc
    12061 ctcagggcag caatacgtgg ccttgttccg ggagcctggc gacctgcccg gcaggaagtt
    12121 ggaggtgccg ggaagccagg cgtcgggggc caggtcgcca ttggtgtcga cgaccacggg
    12181 acccgagctc ttcgtcatcg cgctgtccac gaagctccac ggtatgggtc tggccgggaa
    12241 gatgaagctg cctgtgagct ccagattccg cgcgcgctgg cctctggcgg gcttcacggg
    12301 gatgaaggtc atacggatgt cgaccgcgac gggcacagtg aagctccctc cgcctgcctg
    12361 cgtgcgctgg aggttcatgg tggtcacacg ctgcttgccg ctcagcgaga tgtaagtgtg
    12421 gaacgctccg caggaggtct tgaggggagc gatgcttacc agcgacaacg cccggaactg
    12481 gatgcgcgtc atggccctgc catagtcgtc gaagacagca tcgtcgagcc gctcgaccac
    12541 tgtgtcggcc ccctcgagtt gccctgcgat ctcggtcgcg aggggcaagc ccttgagttg
    12601 gatccggcca ctgaaaggtt tggagctctt gcagaagaat ccggcgggga ttggattccg
    12661 ggcaaagtca taggaggtct tgccgtcggc ggcggtggtg aagacgtcga tgccgcgctc
    12721 gatcacggga gcagcggcca agggggagag gctgagcagg agcgacgaca cgaaccctac
    12781 aaggatcctt ctgtttctca cgcacgtctc cttcgtccaa aggacagtca gggccgcagg
    12841 caccacaccg gcgatgtggt tgcgaaatca gcgagcatga ctccgcggca ttgagccatc
    12901 ctctatttta tttggtgctt gaggagagct ttttcgcgcc cagggtatca gctgagtctt
    12961 gtgagtcttg cgcggagtac gggatcacca gtggccgttg tagtatcgcc cgctcatgga
    13021 gatccaacct cttctcactg acctcgtcga gcgggtgaag cgtgtccaag gcgtccgggc
    13081 catcgtgctc ggcgggtcgc gggcgcgcgg cacgcacacg ccgaagtcgg acatcgacct
    13141 gggcatctac taccacccgg accagccgct cgacctgcaa gcactcgatc aggcctcggc
    13201 cgagctcgac gaccgtcacc aaccggggct cctgacgccg atcggaggat ggggaccctg
    13261 gatcaacggc gggggctggc tcaccgttca ggggcagccg gtggatttcc tttatcgtga
    13321 tctcggcaag gtctccgctt gcatcgaagc gtgctgtgcc ggcgaggtgg agatggtcta
    13381 ccagccgggg catccgcacg gcttctcctc ctctatctat atggccgagg tggccgcctg
    13441 ccagatcctc tgggaggccg atggtcagct ttcagccttg aaggagcgga ccgatcccta
    13501 tccggagccc ttgaagctcg cgctgattca gaaattcggc tgggagatgg gtttctccct
    13561 gatgatcgcc aggaaagcca tcgaacgcgg cgatgtggct tacgtcgccg gctgctgctt
    13621 ccggtgcgtg ggctgtcttc tccaggtggt cttcgccctc aacgggcaat attggatgaa
    13681 cgagaaaggc gcggtggcct tggccgatac ctttctcgtt cgcccggcga tgctccgggg
    13741 gcggatcgaa aaagcgttca cattattggc cgccgaccgg ctcgcgctca cggaggcgat
    13801 cgccgtgctc gacgaggtga agcgcgatac ggacctgttg gctactccaa gaacagatcc
    13861 ggcatgagcg gcgcgccggg ctggacggcg tagcggtcga ggtcggtgat cccggccccg
    13921 gccagcacgt cctcgtcgat gaagaagttg ccggtggtgc tgcggctgtc cttggtaagg
    13981 atgaggtagg cggcgtcggc cacgatctcg ggcttgcggc agttctcagg cttgatccgc
    14041 cctcccagca tggcaa
//
LOCUS       DASPJI010000456         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_323109, whole genome shotgun sequence.
ACCESSION   DASPJI010000456 DASPJI010000000
VERSION     DASPJI010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323109"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="VEL74_12365"
     CDS             <1..1466
                     /locus_tag="VEL74_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HYG63369.1"
                     /translation="AVKARGEQRRARSDWMKVERERGISVTASVMTFEYGGRTFNLLD
                     TPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIETQTRKLFEVCRLRNVPIVTFINKLDR
                     EGRDPFELLSEIEETLQLHVTPASWPIGMGQSFLGCYDLFNERLLLMDRSRRDVVTEG
                     EVCQGLDDPKLDRLLPAGAVARLREEVEMARGLCPEFDLQTYREGHLTPVFFGSALNN
                     FGVRDLLEGLVRIAPPPRPQPAADRVVDPEEDKVTGFVFKIQANMDPKHRDRIAFVRL
                     CSGRFERGMKLLHVRSGKTMSVHNPVLFLARERELAEEAWAGDILGIPNHGTLRIGDA
                     LTEGEELRFTGIPSFAPELLRRVRPEDPMRAKHLGKALLQLAEEGAAQIFKSRRGSEW
                     IVGVAGALQFDVLADRIRTEYNIPVHLESTSLYTARWVEADDPLLLKRFINANDGSLA
                     EDHNGLPVFLARNAWHLKSTGEDWPGMRFLTTREQVQ"
     gene            1597..>2100
                     /locus_tag="VEL74_12370"
     CDS             1597..>2100
                     /locus_tag="VEL74_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63370.1"
                     /translation="MTKLAAFALTGALAFPAFAAADVQPIDDSTLQKATARACQRAER
                     ALLGFEGECTVTSAERRVLRGDIAEYSFDVRVGAGPYDVIGVHRVVRESAPNEPVKTA
                     QAVLFAHGDIWNFDAAFLANPQQALPVFLAQNGLDVWGIDFRWTRVPASVTDFTFMKD
                     WGIEQDAR"
BASE COUNT          326 a          782 c          683 g          309 t
ORIGIN      
        1 gcgcggtcaa ggcgcgcggc gagcaacggc gcgcgcgctc cgactggatg aaggtcgagc
       61 gcgagcgcgg catctccgtc accgcctcgg tgatgacgtt cgagtacggg gggcgcacct
      121 tcaacctgct cgacacgccg ggccacgagg acttcagcga ggacacctac cggacgctca
      181 ccgccgtcga ctcggcggtc atggtcatcg acgccgccaa aggcatcgag acgcagaccc
      241 gcaagctgtt cgaggtctgc cggttgcgca acgtccccat cgtcaccttc atcaacaagc
      301 tcgaccgcga ggggcgcgac ccgttcgagc tgctctccga gattgaggag acgctgcagc
      361 tccacgtgac gccggcctcc tggcccatcg gcatggggca gagcttcctc ggctgctacg
      421 acctcttcaa cgaacggctc ctcctcatgg accgcagccg ccgcgacgtg gtcaccgagg
      481 gcgaggtctg ccaggggctc gacgatccca agctggaccg gctcctcccc gccggcgccg
      541 tcgcccgcct gcgcgaggag gtggagatgg cgcgcggcct ctgcccggag ttcgacctcc
      601 agacctaccg cgaggggcac ctgacgccgg tcttcttcgg cagcgcgctc aacaacttcg
      661 gcgtccgcga cctgctcgag gggctggtgc ggatcgcgcc gccgccgcgc ccgcagcccg
      721 ccgccgaccg ggtcgtcgac ccggaggagg acaaggtcac cggcttcgtc ttcaagatcc
      781 aggcgaacat ggaccccaag caccgggacc gcatcgcctt cgtccgcctc tgctccggcc
      841 gcttcgagcg cggcatgaag ctgctgcacg tgcgctccgg caagacgatg tcggtgcaca
      901 acccggtgct gttcctggcg cgggagcgcg agctcgccga ggaggcctgg gcgggggaca
      961 tcctcggcat ccccaaccac gggacgctgc gcatcggcga cgcgctgacc gagggggagg
     1021 agctgcgctt caccggcatc cccagcttcg cccccgagct gctgcgccgg gtgcggcccg
     1081 aggacccgat gcgggccaag cacctgggca aggcgctgct ccagctcgcc gaggaggggg
     1141 cggcgcagat cttcaagtcg cgccgcggct ccgagtggat cgtcggcgtc gccggcgcgc
     1201 tgcagttcga cgtcctcgcc gaccgcatcc gcaccgagta caacatcccg gtgcatctcg
     1261 aatcgacctc tctctacacc gcccgctggg tggaggccga cgacccgctg ctgctcaagc
     1321 gtttcatcaa cgccaacgac ggcagcctgg ccgaggacca caacggcctc cccgtcttcc
     1381 tggcccgcaa cgcctggcac ctgaagagca ccggcgagga ctggccgggc atgcgtttcc
     1441 tcaccacccg cgagcaggtg cagtagcgcg ccgaacctcc gggcggggtg aggggatcca
     1501 ccccgccgcg gcgtttctct cctcgaccgg gcgagagccc gcaacatcaa ggtaaaacac
     1561 tacttgcagc ctatcgaggc cctataagga gttttcgtga ccaagctcgc cgctttcgcc
     1621 cttaccggcg ccctggcgtt ccccgcgttc gccgccgccg acgttcagcc gatcgacgat
     1681 tccaccctcc agaaggccac cgcgcgggcc tgccagcggg ccgagcgcgc cctgctcggc
     1741 ttcgagggcg agtgcaccgt gacctccgcc gagcgtcgcg tcctccgtgg cgacatcgcc
     1801 gagtacagct tcgatgtccg tgtcggcgcc gggccgtacg acgtgatcgg cgtccaccgg
     1861 gtggtccgcg agagcgcgcc caacgagccc gtcaagaccg cccaggcggt gctcttcgcc
     1921 cacggcgaca tctggaactt cgacgccgcg ttcctggcga acccgcagca ggccctcccc
     1981 gtcttcctgg cgcagaacgg cctggatgtc tggggcatcg atttccgctg gacccgcgtg
     2041 ccggcctcgg tgaccgactt caccttcatg aaggactggg gcatcgagca ggacgcccgc
//
LOCUS       DASPJI010000457        22754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_373517, whole genome shotgun sequence.
ACCESSION   DASPJI010000457 DASPJI010000000
VERSION     DASPJI010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 22754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22754)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22754
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373517"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VEL74_12375"
     CDS             <1..227
                     /locus_tag="VEL74_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010150604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HYG63371.1"
                     /translation="TERAIQQNLRTLTAGRTALIIAHRLSTIRHADRILVLRDGQVAE
                     EGDHDDLLARGGTYADLWHVQAGDLVESGV"
     gene            345..1346
                     /locus_tag="VEL74_12380"
     CDS             345..1346
                     /locus_tag="VEL74_12380"
                     /inference="COORDINATES: protein motif:HMM:NF016629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HYG63372.1"
                     /translation="MGNHDLSYRLMFSQPRMIEEMLREFVAESWVETLDFSTLERVNA
                     DFVTPGLKGREGDLIWRLRRQSGRLVYIYLMVEFQSKIDRFMAVRLTSYVSLFYQDLI
                     SRGELSPAGRLPLVIPIVLYNGEPGWSAPLDLAELIDSVEPELEPYLPRLHYKVIDEG
                     SYTPEELRQRQSVPALLFWLEKSRKPQDLRWGVQGLVERLDRPGDAGLRRAFLVWLDR
                     VLMPDHDTVERIPEALGLEEFQDMLEKRVLEWNEELREAGRQEGRQEGRQEGRQEGRQ
                     EGRREGEAALLIRQLTLKFGPLSEETRGRVLSADPELLLEWGERILTARRLPDIFGE"
     gene            1382..2959
                     /locus_tag="VEL74_12385"
     CDS             1382..2959
                     /locus_tag="VEL74_12385"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HYG63373.1"
                     /translation="MRSAWLLLLACLAPLSPGAIIAAEAPRYDLVIRGGTVVDGSGSP
                     GFRADVAVRDGKIARVGDLAGVKAARVIDASGLVVAPGFVDVHTHADDLADKPLAENF
                     LRMGVTTIVAGNCGSSPVDVGASLAKLRQGGASVNFGTLLGQGSVRQEVMGGERRAPT
                     AAEMEKMKGLVAKAMADGALGLSTGLQYVPGSYAEPNEIVELARVACRAGGLYASHMR
                     NEGTEIEAALAETLAVGEEAGCPVQVSHLKIDSPNRWGVSGKALAMIDAARARGVDVA
                     ADQYAYTAAGSSLSIRFPDWALEGTDEEVRRRFADDATWRRIEAGLRELLAARGFSDL
                     SFATVAAYEPDPSLQGLTMKQVAEKLRGDGSAASQFETARRMMLEGGAGMVYHLMSEE
                     DVERVMRHPRVAVASDSSVLPFGEGRPHPRGYGNNARVLGHYVRDKKVIGLEEAVRKM
                     TSLPAAHFKLAGRGLVREGNAADLVLFDPAKVADPSTFEKPHAYAAGFPYVIVNGVPV
                     IDAGRHTGAKPGQVLRR"
     gene            3309..3650
                     /locus_tag="VEL74_12390"
     CDS             3309..3650
                     /locus_tag="VEL74_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63374.1"
                     /translation="MKRLSRLLLAAVLLVAAGAIPHVSNAASSVVPGTEVAGPVKPEP
                     IASTCGYSLGDEENAQSDAEGNAHRYCQAMGCKGAIITTVHPAWTDKQGYTHVCVTFA
                     CDCSVVVVAAK"
     gene            complement(3714..3935)
                     /locus_tag="VEL74_12395"
     CDS             complement(3714..3935)
                     /locus_tag="VEL74_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015228440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HYG63375.1"
                     /translation="MPELLDRITLDPEVRSGKPVIRGTRITVADILEYLAGGMTTEEI
                     VADFPDLSADDVRASLAFAALRERRLFSS"
     gene            complement(4069..4659)
                     /locus_tag="VEL74_12400"
     CDS             complement(4069..4659)
                     /locus_tag="VEL74_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63376.1"
                     /translation="MTSFSQSSTVSAHLPNAFTTTFLRRLAERDEPPTAGDADVAGPW
                     RIEPIPGHGFGLFRVGESLARGFAPTAVFPSRWLALLMAGVLPGTGKDPILLLSKQPD
                     AHGVYPVALAGGSDGGDGGDGDGPGDRVPVGFLQLFDERAVDALNVVIHLLQAPECLA
                     YVLEAAGGVALERCGAILDERLPEVGAEDADGAAAG"
     gene            4918..5253
                     /locus_tag="VEL74_12405"
     CDS             4918..5253
                     /locus_tag="VEL74_12405"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HYG63377.1"
                     /translation="MSLAGAKSEPIPIVADADGVLRVEGTRVPLATVVEAFREGATPE
                     EIVLRYPSLDLAGVYSVIGYVLHHRAEVDAYLDKQRRQSEAVRAENEARFDPTAVQER
                     LIKWRRQAG"
     gene            5426..5869
                     /locus_tag="VEL74_12410"
     CDS             5426..5869
                     /locus_tag="VEL74_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63378.1"
                     /translation="MGSDEKIKPSDLFDLFSRMKGGLDAVRRVMGGRMSSTDIQVTVS
                     PSGISVQPYSASVHAGGTVRWNVAVEGEVAPFRLTIYFSEESPFRWSSRSLTLRSGSD
                     FSRGMSVSASATRPGDYKYGVRGVIVGSGELIDDDDPYLEVLAQP"
     gene            5886..7361
                     /locus_tag="VEL74_12415"
     CDS             5886..7361
                     /locus_tag="VEL74_12415"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYG63379.1"
                     /translation="MYPIEVMVAEAELASLLDRAMVSLNALQKEFYFHRPSPELEAQV
                     GLLRGTPHHAPELFGWLESYRARAGGSRPYLILVVAGRLSTASYENAFGSHRAAQGLA
                     VFTLYDRGRFVADAVRFIRYYLVRYAISFIAPSLGTHEETRGCFLDRKEYKPSIQESL
                     LSGGICDQHRRELRAHISPQVHASLELMCKRVAEDLPYAIVMKGGGVKGLALTGALEV
                     LSQYLSFDVFVGTSAGAMTAVLLAAGYTPPELREELYELDFSRFCDSGLFRIASNILR
                     RGALHSGDSMQDWLEERLVARCGIPPPVRLSDLPSRAVVYACSANGVLAFDKLGDRAS
                     EPASFAVRCSAAIPFFFASPLMAGVRAFDGGLWQNFPLMRFIADNPQKPVIGLYLKPQ
                     FMRKRSLVSDLIESATVGEEPDVIRKYANQIVAIDPFPVRTTDFSISRNEKSFLLAAG
                     RFAAIDFLVKNLPDEAPGSSVLQEAQQQLMSLRASIDRKKL"
     gene            complement(7851..8240)
                     /locus_tag="VEL74_12420"
     CDS             complement(7851..8240)
                     /locus_tag="VEL74_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63380.1"
                     /translation="MSDETTQTGRVGDWLRLGEVMESNTAELSHLEPFRVRLTEMTRD
                     VRELFKEQAALKASKQDVSKRLDVLLREGDRLATLIRQALRQHYGIRSEKLTEFGLQP
                     FRGRKPRTKKRSTVAPAPEAAAPIDPS"
     gene            8641..8877
                     /locus_tag="VEL74_12425"
     CDS             8641..8877
                     /locus_tag="VEL74_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017326253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChpI protein"
                     /protein_id="HYG63381.1"
                     /translation="MKTAVSLPDPLFEAAERLAERLGVSRSELYAAALESYLRSHQGE
                     EITAALNRVYAEEDSSLDEGWAALQAGSLPRDAW"
     gene            8932..9390
                     /locus_tag="VEL74_12430"
     CDS             8932..9390
                     /locus_tag="VEL74_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain protein"
                     /protein_id="HYG63382.1"
                     /translation="MRARIYVDASVVGGCEDDEFAAYSLRLMESFVRGDFVLVMSTLT
                     IQELAAAPAEVRRHLASVPERHIETLQLDEEARELAETYISEGVIMARMRADAQHIAI
                     ATVARVDVLVSWNFKHIVNLHRIHGYNSVNLRRGYPTLEIRAPREVLLDE"
     gene            9662..14095
                     /locus_tag="VEL74_12435"
     CDS             9662..14095
                     /locus_tag="VEL74_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HYG63383.1"
                     /translation="MSPSTSSTIAVVAEKPAVARDIARVLGADRRGEGCFQGNGYVVT
                     WAIGHLVTLAEPHEIRPEWRKWRRDHLPMLPRDWPLVVPEATKQQFEAVRKVINAPSV
                     ERVVCATDAGREGELIFRYIYEAAGCSKPVSRLWISSLTPDAIRKGFQRLRDGHDYDP
                     LADAARGRSRADWLVGMNLSRAYTLDFNEDLSVGRVQTPTLAMVVERELAIRAFVPED
                     YLEVVATFTPDLEAAEPRTYRGTWFRGDSPTAEAKRLQSEKGKEGAEFAEAAGIVARA
                     KSGKAQVESVRAETKRMPPPLLYDLTELQRHANRLFGWSAKKTLGIAQGLYESRKLIS
                     YPRTDSRHLSADVAATLPEIVRAVSGPYQDRFAPGTGERPLSRRFVDDSKVTDHHAIL
                     PTTTSAEGMDLSADERRLYDLICRRLLSAWHDEHVWSVTTVITRITSGEVIDRYHSTG
                     TAVEQVGWKVLDLDRKKDKKAREKKEAKAEGGEEPDEPADSTDDESQDLPPGLVQGQP
                     QRVLDAKALAKKTRPPKRFTEATLLTAMETAGKTLDDKELSEAMKESGLGTPATRAEI
                     IETLVRREYVVRQGKSLEATDKGIRLIQVVHPHVKSPAMTGQWEAHLQSIQRGQADLA
                     TFMEKIEAYIKEVVGPSPPGPLSRLPDPLPGRGGDFSGRTAPDRGGSRSPSPNFVPQG
                     AGVPPPLGGRAAGSGGGQEGGSPAYGRQLRLVDSAAGGGPRSAQPSPPTQGRQLRLDG
                     FSPPASRPPSREGASPSPTQRFGSGAMPNQSAPPRQAPPASFQGDRTPTPPERLTDLL
                     RSAFRLQEFRPFQEAVCRTVTAGHDALLVMPTGAGKSLCYQLPGLARAGTTLVVSPLI
                     ALMEDQVAKLRELGLRAERIHSGRDRGASRQVCIDYLQGKLDYLFIAPERLSVPGFPE
                     MLARRKPALVAVDEAHCISHWGHDFRPDYRLLGQRLPLLRPAPVIALTATATPLVQDD
                     IATQLGLGGTERFIHGFRRTNIAVEVAELRPSARAEAVRRVLAEPGRRPAIVYAPTRK
                     EADALGELLQEDFPAATYHAGMTTAARDRVQSAFLSGALEVIVATIAFGMGVDKSNIR
                     TVIHTGLPGSLEGYYQEIGRAGRDGLPSRAILLYSFVDRRAHEFFHGRDYPDIDILTR
                     IHKALTDEPQTPGRLRKKLKIDEEEVFERALEKLWLHGGALIDADDQITRGGDGWRLP
                     YTAQSEHKLAQIDQMVRFAESHGCRMLHLVGHFGDQEDSGAPCGMCDVCAAAECVVRS
                     FREPTVAEGVVLRRIVETLAERRGASTGQLYREVCGDGSPHPMDRRSFEHLLGGLAAA
                     GLVRMMEDSFEKDGKLIHFQRAALTLEGHRAGPDGAVGVSLADQPPPPPKKKRSREKK
                     ELKKERVGERPVRVSPRVVEGEEAGEALPVLVEALRAWRRGEAQRRKVPAFRILTDRA
                     LTALAAARPADEAELLGIAGIGPTIVKKYGRELLDLLRAGG"
     gene            complement(14316..15446)
                     /locus_tag="VEL74_12440"
     CDS             complement(14316..15446)
                     /locus_tag="VEL74_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63384.1"
                     /translation="MTRRLVGNLLFEEELAAPRRSPSSSVLETAAGAATLLRAFAREG
                     DRLWTPAPVDPACLAPLPGIPVPILESGPLASLPPAGELLAWGESPAVAALRPATPPP
                     PPDLNAPLHELLWRVPPAPPGVVAAVHHRSFCLAVARDLDQALPGARMLSSPDELDRH
                     LTTFQRSWVLKAPLSAAGRSRYIHRPGESLSDPAVRRRVENLFARHGSLLFEPWMERT
                     DDFGCVAVLTSSGLRVAGFHRLLVDRRGQFTGIETTASYDGGFPNLSASEGRQMEEAL
                     EAAAGALRRAGYSGPFGLDAWRYQSPDGGTGFNPLGEINARLTFGFVARSLADRLREP
                     LGLPPDHPLRLRLGRPPGPRKTGSVLPLFQLENGYSAAWIEN"
     gene            complement(15443..15679)
                     /locus_tag="VEL74_12445"
     CDS             complement(15443..15679)
                     /locus_tag="VEL74_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HYG63385.1"
                     /translation="MIQELDTVALTQDVEAHGLERGDIGTVLHVYTRETAFEVEFVTG
                     GGSTVALLTLGADAIRPIGKSEILHVRELTESQP"
     gene            complement(15707..16489)
                     /locus_tag="VEL74_12450"
     CDS             complement(15707..16489)
                     /locus_tag="VEL74_12450"
                     /inference="COORDINATES: protein motif:HMM:NF016212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HYG63386.1"
                     /translation="MEIRDFARTVLETEDLDVKLAPPAGPLTDEDPGPAYRAAGPGRP
                     ANLRLPPPSRVNVPSIEGMPDPAQRPRILHALANHELQAAELFAWALLAFPEAPAGFR
                     RGLLHILQDEQRHTRMYRARLRAWGADLGDFPVNGYFWSKTPAIQSPLDFVCAMSLTF
                     ENANLDHTVEYSEAARRADDPKTAAVIDEVHRDEVEHVRFGWTWLHRFKGEDESAWDA
                     FQSHLTWPLRPAKAKGLRFHRAGREAAGLDPDFIRRLEESQP"
     gene            complement(16493..17752)
                     /locus_tag="VEL74_12455"
     CDS             complement(16493..17752)
                     /locus_tag="VEL74_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HYG63387.1"
                     /translation="MAFTEQDCLKCRRDFPALERSFEGRPLAFLDGPAGTQVARQVID
                     AISGYYATSNANLHGFFPTSRETDRLLIETREAVAAFLGAPSWREISFGQNMTTLTFA
                     LSHALAREMKPGDEIVITQLDHESNRGPWLHLKECGHERGITVREVALRPDGTLDPDD
                     MARQINGRTRLVALGGASNALGTVNDVVLARRLSREVGAWLLIDAVHYAAHFSVDVAA
                     LDPDFLLCSAYKFYGPHVGLLYCRPGLLDRLRTDKLSTQEDEAPSRIETGTLNHAAIA
                     GVKAAIDYIASWGEGQTLRERLVSAMEGIAAWEHSVAAYYHDNVRQIPGVRVWGPDFT
                     SPRRAPTVSITVDGVPSSEVAARLGEQGLQVWDGHFYAARAIEVLGLQEKGGVVRTGV
                     MMYNTREEIDRLLEAVAAVAAGRLVSA"
     gene            complement(17775..19067)
                     /locus_tag="VEL74_12460"
     CDS             complement(17775..19067)
                     /locus_tag="VEL74_12460"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYG63388.1"
                     /translation="MPRRAFYLVVLGLVFWAAPVRAADPPPPVIAAVGDIACDPGDPS
                     FNATNGTATACRMRATSDLLVGRDLAAVLALGDTQYENGTAAKFMASYDPTWGRLKAI
                     TRPVVGNHEYLTPGAAGYYSYFGAAAGDPAKGYYSFDVGSWHVIVLNSDCAQVGGCGV
                     GSPQEQWLAADLAANPGRCTLAAWHHPRFSSGPHGNDASTDAFWRALHAAGADVILTG
                     HDHIYERFAPQDVDGNADLGRGIRQFVAGTGGKNLTSFPVVRANSEARSAAAFGVLEL
                     TLRPTGYDWRFRPAAGSTFTDFGSAACHSAPPPPATELHTVTPCRIADTRDADGPALA
                     ANTDRRFQMAGRCGVPAEASAVAVNVTVVQPTTRGGLRLYPGDQGTPLTSTINFRTHR
                     TRANNAIVGLGADGSVGVRCDMASGSTHMVLDVFGYFQ"
     gene            complement(19115..21835)
                     /locus_tag="VEL74_12465"
     CDS             complement(19115..21835)
                     /locus_tag="VEL74_12465"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HYG63389.1"
                     /translation="MSVRSRASFRLLVCLFILLLAVPVLAQETGSISGVVQSGDGSPL
                     PGVVVTASGPLLPGGRTATSASDGSYSILRLPPGPYTVSAELSGMGNTRREVIVAVAR
                     DTQVGLTLSPATVQDEITVSAVAPVVDVRSTEIQVNYTHEFIENLPVPRTYKGLFQLA
                     PGVSENDRLTPNAGGSRMDNNFLIDGINITNPHFGDILPNITELDFEEVAIKRGGITA
                     EFGRTGGMVVNAVTKSGTNELAGEARLEYQPNDFVSDSENPNVSNTPDRSGVAASLGG
                     PLLRDHLWYYGSASFPRQSTTERTNQFGSIPDLEVNTDEYFLKFTSQPVQNHYLNISG
                     RSREVSSENEGLDVNVSPTVRRTTITDYLLATLGWTWNTTENSFLELKLNYNKEENSV
                     DPATDLGYRPAFNAARPDLVGRFTSTDDRRPGGANRTGQLVGGTDLAINDQNFERNEA
                     RATFQSFQDWFGVSHDLRAGVTYDENEETLNRRANGWGVITWNAATQQFSATYVSQQP
                     PHTGRGEALGLFAQDQFTLGQRTTIVAGVLVNRDTYYGEALGSTPGTKREVKLLTFDW
                     DQQIQPRLGVSFVPSLERGDKLYFNYGRYFNTENKSLVRAASPTRIFSTTARFDAAGN
                     FISETAQANTQSKTIDPGLDPQFTDEFVAGYARPFGRGWAAEIWGMYREVGDIMEDIS
                     RDGLGNGPFRVAQLPDAYREYTAATLQVSRRPIDGRWLGLALEASYTWSRLEGNWDID
                     FSSGDSPFYNSSFLGDGPGVLLTDNRDGLMRGDRTHVAKVFATVEPVERLRVGTYLRY
                     QSGAAWEARSVPAATVSSASHIAYLERAGSRRMDSWFNTDLLASYEFPLSIVGLELEA
                     RMTNVFDEQAQLAVDDRLLLGRAMTPNPDFGTTTTYSTPRSFVLSAIIRY"
     gene            complement(21932..22447)
                     /locus_tag="VEL74_12470"
     CDS             complement(21932..22447)
                     /locus_tag="VEL74_12470"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HYG63390.1"
                     /translation="MTLPIDQKEETSPMATAEERQKQIDRIEELPRLLEEAVRGLDDE
                     QLDTPYREGGWTVRQVVHHLADSHANAVTRMKLILTEDHPTLKTYDQDEWAGLADSAL
                     PLEPSLAIVRGLHQRCTALLRAVPEEAWPRTAFHPERGDITLDDLLGLYAGHGANHVR
                     QITDLRARQGW"
     gene            complement(22444..>22754)
                     /locus_tag="VEL74_12475"
     CDS             complement(22444..>22754)
                     /locus_tag="VEL74_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63391.1"
                     /translation="SHAVIKRPKSGDFRVQIDHGGSAAPADPPAAVLERAERILAAVA
                     GPWLYARVDGCVLSGRRGFHLMELEMLEPSLFFAQDPLAPGRFAAALAGVLGRDRAAA
                     "
BASE COUNT         3626 a         7386 c         8078 g         3664 t
ORIGIN      
        1 agaccgagcg ggcgatccag cagaacctgc ggacgctgac cgccggccgc acggcactga
       61 tcatcgccca ccgcctctcg accatccgtc acgccgaccg catcctggtc ctgcgcgacg
      121 gccaggtcgc cgaggagggg gaccacgacg acctcctcgc ccgcggcggc acctacgccg
      181 acctctggca cgtccaggcg ggggatctgg tggagagcgg ggtgtgagag gcctttccag
      241 gtgacctacc gtcattagtc tcttcaggga aggccgcgcc tcgcgctgcc gcatgctaaa
      301 gtggcgcccg ttcgtaccca attcatggac tcgaggagga cgatctgggg aaccacgacc
      361 tcagctatcg gctgatgttc tctcagccgc ggatgatcga ggagatgctc cgcgagttcg
      421 tcgccgagag ctgggtcgag acgctggact tctccaccct ggagagagtc aatgccgact
      481 tcgtcacccc agggctcaaa ggtcgcgagg gagacctgat ctggaggctc cgtcgccagt
      541 ccggccggct ggtctatatc tatctgatgg tggagttcca gtccaagatc gaccggttca
      601 tggccgtgcg cctgaccagc tacgtgagcc tgttctacca ggatctcatc tcccgtggcg
      661 agctatcccc cgccggccgg ctgccgttgg tcatccctat cgtcctctac aatggagagc
      721 cagggtggtc ggcgcccctg gatctcgccg agctgatcga ctcggtggag ccggagctgg
      781 agccctatct cccccggctg cactacaagg tcatcgacga ggggagctac actcccgagg
      841 agctgaggca gcggcagagc gtgccggccc tcctcttctg gctcgagaag agccggaagc
      901 ctcaggatct caggtggggc gtccaggggc tggtcgagcg tctcgacagg cccggggacg
      961 ccgggctgcg ccgggccttt ctggtgtggc tggatcgggt cctgatgccg gaccatgaca
     1021 ctgtcgagcg gattccggaa gctctcggac tagaggagtt tcaagacatg ttggagaaga
     1081 gagtcctcga gtggaatgaa gagctgagag aagcgggccg ccaggaaggc cgtcaggaag
     1141 gccgccagga aggccgtcag gagggccgcc aggagggccg aagggaggga gaggctgcgc
     1201 tgctcatccg ccagctcacc ctgaagtttg gacccctcag cgaagagacc cggggccggg
     1261 ttctctccgc ggacccggag cttctgctgg aatggggcga gcggatcctc accgctcgcc
     1321 ggctgcccga catcttcggc gagtgagccg gccccggtcc gccgcgacgg tctacaccgc
     1381 gatgagaagc gcctggctcc tcctcctcgc ctgcctcgcc cccctctccc cgggagcgat
     1441 catcgctgct gaggctcccc gatacgacct cgtgatccgc ggcggcaccg tggtggacgg
     1501 ctccggctcc cccggcttca gggcggacgt cgccgtccgt gacggcaaga tcgcccgggt
     1561 gggggacctc gcgggcgtca aggcggcgcg ggtgatcgac gcctccggtc tggtggtggc
     1621 tccgggattc gtcgacgtcc acacccacgc ggacgatctg gccgacaagc cgctcgccga
     1681 gaacttcctg cgcatggggg tgacgacgat cgtcgccggc aactgcggct cgtcgccggt
     1741 ggacgtcggc gcctccctgg cgaagctccg gcaaggcggc gcttcggtca acttcggcac
     1801 cctgctgggc caggggagcg tccggcagga ggtcatgggc ggcgagcggc gggcgcccac
     1861 ggcggcggag atggagaaga tgaaggggct ggtcgccaag gccatggccg acggcgccct
     1921 gggtctctcc accggcctcc agtacgtgcc gggctcctat gccgagccga atgagatcgt
     1981 cgagctcgcc cgcgtcgcct gccgggccgg cggcctctac gcgtcgcaca tgcgcaacga
     2041 ggggacggag atcgaggccg cgctcgccga gaccctcgcc gtcggggagg aggcgggctg
     2101 cccggtgcag gtctcgcacc tcaagatcga cagccccaac cgctgggggg tgagcggcaa
     2161 ggcgctggcg atgatcgacg ccgcccgcgc ccggggcgtc gacgtggccg cggaccagta
     2221 cgcctacacc gccgccggct ccagcctctc catccgcttc cccgattggg cgctggaggg
     2281 gaccgacgag gaggtgcgcc gccgcttcgc cgatgacgcc acgtggcgcc gaatcgaagc
     2341 gggtctccgt gagctgctgg ccgctcgggg cttttcggat ctctccttcg ccaccgtcgc
     2401 cgcctacgag ccggacccct ctctccaggg gctgaccatg aagcaggtgg cggagaagct
     2461 gcgcggcgac ggctcggcgg cgtcccaatt cgagaccgcc cgccggatga tgctcgaggg
     2521 cggcgccggc atggtctacc acctcatgtc cgaggaggac gtcgagcgcg tgatgcgcca
     2581 cccccgggtg gccgtggcct cggacagctc ggtcctcccc ttcggcgagg gtcgccccca
     2641 cccgcgcggc tacggcaaca acgcccgggt gctcggccac tacgtgcgcg acaagaaagt
     2701 gatcggtctc gaggaggcgg tccgcaagat gacctccctc cctgccgctc acttcaagct
     2761 cgccggccgc ggcctggtgc gggaggggaa cgccgccgac ctcgtcctct tcgatcccgc
     2821 caaggtcgcc gatccctcca ccttcgagaa gccccacgcc tacgccgccg gcttccccta
     2881 cgtgatcgtc aacggcgtgc cggtgatcga cgccggccgg cacaccgggg cgaagccggg
     2941 ccaggtgcta cgcagataag agggaggctg gcctcccgga cggctcctga ggcgtccgag
     3001 cctcgcggat gccgtccgga cgccgcggat tcgatcccga gcgctcggga cgcttcccgg
     3061 agcgtctgga ttccatccgg gcggctcgga cgcgatccga agcgttcggg aagcgtccgg
     3121 gccgtttgga cgccgtccag acgccccgga tgacatccgc ggcggccggg acggcatccg
     3181 gagcgtccgg atgagatcca gaccatccgg gagagatccg cggcctctgg gggctctccg
     3241 tctctccccg gccgcaccgt cttctgaagg agccatccgt gtcggttccc gaaaaggaga
     3301 ctgtgaccat gaagagactg tcccgtctgt tgctcgccgc cgtcctgctc gtcgccgccg
     3361 gcgccatccc gcacgtttcg aacgccgcct cctcggtggt ccccggcacc gaagtcgccg
     3421 gccccgtcaa gccggagccc atcgcctcga cctgcggcta cagcctcggc gacgaggaga
     3481 acgcccagtc ggacgccgag ggcaacgccc accggtactg ccaggccatg gggtgcaagg
     3541 gcgcgatcat caccaccgtt catcccgcgt ggacggacaa gcagggctac acccacgtct
     3601 gcgtcacctt cgcctgcgac tgctcggtcg tggtcgtcgc cgcgaagtga gctgagcgct
     3661 ccgggcggat cgatggaccg cccggagcgg ccgtcaggag cggtctttgc cggttaggat
     3721 gagaagaggc gccgctcacg tagggcagcg aaagccagag aggcgcgcac atcgtccgcc
     3781 gagaggtctg ggaaatcggc aacgatttcc tccgtggtca tcccgcccgc caggtactcc
     3841 agaatgtcgg cgaccgtgat cctcgtgccc cggatgaccg gctttccgct acgcacctcg
     3901 ggatcgaggg tgattcggtc cagcaactcg ggcatgactt gcctcccttc agccgacgct
     3961 gcaagcgtac tcggccctgc ggggcaggca aggcgctcga ggtgtggaaa cgaaggcacc
     4021 accgacaggc tcggcccgcc ggtggtgcct ctctatctaa aggatgtcct acccggcagc
     4081 cgccccgtcg gcgtcctcgg ccccgacctc cggcagccgc tcgtcgagga tggcgccgca
     4141 gcgctcgagg gcaaccccgc ccgccgcctc cagcacgtac gccagacact ccggcgcctg
     4201 gaggagatgg atgacgacgt tcagcgcgtc gaccgcccgc tcgtcaaaga gctgaaggaa
     4261 gccgaccgga acgcgatctc cggggccgtc gccgtcaccc ccgtcgccgc cgtcactgcc
     4321 accggcgagg gcgacgggat agacgccgtg ggcgtcaggc tgcttggaga ggaggaggat
     4381 cgggtccttg ccggtgccgg gaaggacgcc cgccatgagg agcgccagcc agcgggacgg
     4441 gaagaccgcc gtcggggcga agccgcgggc caggctctcg cccacccgga acaggccaaa
     4501 gccatgaccc gggatgggct cgatccgcca aggcccggcc acgtcggcat cgccggccgt
     4561 aggtggctcg tcccgctcgg ccaggcggcg gaggaaggtg gtggtgaagg cgttcgggag
     4621 gtgggcggag acagtgctac tctgagagaa gctggtcata gtccctcctt tggactatgg
     4681 gcggtcaggc ccggggtgag tttggcgctc accttcgggc cgctagcagg attgccgacc
     4741 gccctttgca gcgcttctac atctggatgc ctgcgacacc tttggagcga ccggaggtcg
     4801 cctttggagg tgcgagggaa gcatatgata ggcgggaggg tggttgcaag gggtgctaac
     4861 gaaattcttc ggctcgacgc atcagggctg gtaagatcac cgaggagagg aggatgtatg
     4921 tctctggcag gggcaaagag tgagccgatt cctattgtcg ccgacgccga cggggtcctg
     4981 cgcgtcgaag ggactcgtgt ccctctcgcg acggtcgttg aggcctttcg tgaaggagcg
     5041 accccagagg agatcgtgct ccgataccca tcgctcgatc tggccggtgt ctattctgtg
     5101 atcggctacg tcctccacca ccgggcagag gtggatgcct acctcgacaa gcagcgccgt
     5161 cagtccgagg ccgtccgggc cgagaacgag gcccgcttcg atccgaccgc agtgcaggag
     5221 cgcctcatca agtggcggcg ccaagccggg tgaactgaat gctgcgtctg gcagcggacg
     5281 agaacttcga taacgacatc ctgcgcggcg tcctgcgtcg gtacgccgag tgatgatgtg
     5341 cgcatccagg atgaagacct tgcggagcca gtgacctgcc ctgtaatggg gccatcgtga
     5401 ggtgcagatt ggctgaggac tgatcatggg gagtgacgag aagattaagc cgagcgatct
     5461 ctttgattta ttcagcagaa tgaaaggcgg cctggacgca gtccggcgtg taatgggcgg
     5521 aaggatgagc agcaccgata tccaggttac ggtatcacct agtggaatat cggtccagcc
     5581 ttattccgct tctgttcatg ccgggggcac agtcaggtgg aatgtagctg tcgaaggaga
     5641 agttgcgcca tttcggctta caatatactt ttctgaagaa tcacccttta ggtggagttc
     5701 gagatccctc acgctcagga gcgggagcga cttcagcagg ggaatgtcag tgtctgcgtc
     5761 agccacgcgg ccgggtgatt ataagtacgg ggtgaggggt gtgattgtcg gaagtggcga
     5821 gctcatcgat gacgacgacc cttaccttga ggtgcttgcc cagccgtagg gtttggagga
     5881 atccaatgta tcccattgag gtcatggtcg ctgaggccga gcttgcctca cttctggatc
     5941 gggctatggt ctcactcaac gcattacaga aagaatttta tttccacaga ccttcaccgg
     6001 aactggaagc gcaggtcggc ttgctgaggg ggactccgca ccacgcaccc gagctttttg
     6061 gttggcttga gtcgtaccgc gccagggctg gcggaagtag gccttacctc atccttgtcg
     6121 ttgcggggcg cctaagcaca gcaagctacg agaatgcctt cggaagccac agagcagctc
     6181 aagggctcgc agtattcact ctgtacgata gaggacgctt cgtggctgac gccgtacgct
     6241 tcatccggta ctacttggtt cgttatgcca ttagttttat agctccgagc cttggcactc
     6301 acgaggagac gcgcggctgc tttttggatc gtaaagagta taagcccagt atccaggaga
     6361 gcctgctgtc gggaggcatc tgtgaccagc accgccgcga actgcgggcc catatttccc
     6421 ctcaggtgca cgcctctcta gagttaatgt gtaagcgagt agcagaagat ttgccctatg
     6481 ctattgtgat gaagggtggt ggcgtgaagg ggctggcgtt aactggcgct ctcgaagttc
     6541 tgtcgcagta cttgtcgttc gatgtcttcg ttggtacttc tgcgggcgct atgacagctg
     6601 tgctccttgc tgccggctac acacctcctg aattgcgaga ggagctttat gagctagatt
     6661 ttagccgctt ctgcgactcg ggcctttttc gtatagcgtc aaacatcctg aggcgggggg
     6721 ctcttcattc aggggattct atgcaagatt ggctcgaaga gaggttggtg gctaggtgtg
     6781 gaattccgcc gcctgtgcgg ttgtctgacc ttccaagtcg agctgtcgta tatgcgtgct
     6841 ccgcgaacgg tgttctggca tttgacaagc tgggtgatag agcaagcgag ccagcgagtt
     6901 tcgctgtgcg ttgcagcgcg gcgatcccat tcttctttgc cagccccttg atggcgggag
     6961 taagggcgtt tgatggtggc ttgtggcaga actttcctct aatgcgcttt attgcggaca
     7021 acccgcagaa gcctgtcatc ggcctctacc tcaagcctca gtttatgagg aagagatctc
     7081 tggtgtccga tttaatagag tcggctaccg ttggagagga gccggacgta atcaggaagt
     7141 acgctaatca aatcgtagct atcgatcctt ttcctgtcag gacaacagat ttttctatat
     7201 cccggaatga gaagagcttc ttgctggcgg ccggacgttt tgccgcgatc gactttcttg
     7261 tgaagaactt gcctgatgag gccccgggta gtagcgttct ccaggaagcg cagcagcagt
     7321 taatgtcact tcgcgcctcc atcgatcgca agaagctttg aagtctccgc agcctggagg
     7381 cattgtttat tgtaactagc tcatgccagg cttatctcgg cccctataaa gttgtcccgg
     7441 ttccgataag cctatctcgc tcccgctagc gtcagctcgg atcgccaagc atatctcgtt
     7501 tcagatacgc tcatcttgct ccagacaatc gtagcgggag gtctgctgag cgtgtcccgc
     7561 gccagcctga cgctagctct gcgcagataa gcttgagttg ttacaaaaga gcgtagatcg
     7621 tcctgctgag cgtattccga tcccactaag ccgagcttgt tccggcctaa actcatctcg
     7681 ggcgcgataa cgctacgaga tccgatggaa gctcccctcg accggcggaa ggagatgtcc
     7741 atagggtggg cgggccgtga tgactatcgc tggcgagtgg cggggcagga ttcggcagcg
     7801 gcggatgggg ggagaaaggg agcgtgccgg agcacgcccc cggagcaacg ctagctggga
     7861 tcgataggag ccgccgcctc cggggccgga gccacggtgg aacgcttctt cgtgcggggt
     7921 ttgcggccac ggaagggctg aaggccgaac tcggtgagct tctccgagcg gataccgtag
     7981 tgctgccgga gcgcctggcg gatgagggtg gctaggcggt caccctcccg caggaggacg
     8041 tcgagccgct tggagacgtc ctgcttgctg gccttcaagg cggcctgctc cttgaagagc
     8101 tcccggacgt cccgggtcat ctcggtcaac cggacgcgaa agggctccag gtgcgacagc
     8161 tcggcggtgt tggactccat cacctccccg agccgcagcc agtctcccac tcgtcccgtc
     8221 tgtgtcgttt cgtcggacat acaaccatct cctttctggg ctcccacagg gacccgatga
     8281 gccggcgccg gagatcggcg ccggatgatg cgaccggcag cgacggagcg gagagtagac
     8341 ccgcactccg gagcttgtca atacccgcag gatcgaatat ggtccacggc gccggcagcg
     8401 ttccagggcg gcggctctgg gttcattgac aactgaatcg tagtgggctc ccctgcttcc
     8461 gcgaccgggc gggggagccc catggccgcg gcggctaacc ggatccgctc ctccggtcta
     8521 agtggaggca atccgacaag ttagtctctt cagaggtggt cacagatcgt gaccacctca
     8581 agggctttgg cctgggaaga gcccgatgag agccggcatg actggagtca tactaccctc
     8641 atgaagacag ccgtctctct cccggaccct ctcttcgaag ccgccgaacg gctggcggag
     8701 cggctcggag tttcccgtag cgagctttac gcggccgctc tcgagagcta tctccggtcc
     8761 caccagggcg aggagatcac cgcggccctg aaccgggtct atgccgagga agactcgtct
     8821 ctcgacgagg gctgggcggc gcttcaggcc gggtctctgc cacgggatgc ctggtgatcc
     8881 ggcgcctctg gtctcctgaa ggcctccccg cctgatcggt atactacgag catgagggcc
     8941 cggatctacg tcgatgcgtc cgtggtcgga ggctgcgagg acgatgaatt cgcggcgtac
     9001 tccctccggc tcatggagag cttcgtccga ggcgactttg tcctcgtcat gtccaccctg
     9061 acgatccagg agctggcggc ggctcccgcc gaagtgcgga gacatcttgc ctcagtgccg
     9121 gagaggcaca tcgaaactct ccaactcgac gaggaggcaa gggaacttgc cgaaacctac
     9181 atcagcgagg gggtcatcat ggccaggatg cgggcagatg cccagcatat cgccatcgcc
     9241 actgtggccc gcgtggatgt gttggtgagc tggaacttca agcacatcgt caacttgcac
     9301 cggattcacg ggtataactc cgtgaacctg cgcagagggt atcctacgct tgagattcgg
     9361 gcgccaaggg aggtgctgct cgatgagtag aagcgagaag aagtttgacg cagttgctat
     9421 gatgcgatcg attcgggaag aggtctcggc caagatcgaa ggcatgacgc tggaagagga
     9481 gttggagtgg ctggcttcgc aggagcttga agatccgttc ctccggcgtc tccgtgacag
     9541 ggccacacaa ccggctgttc cagtaggccg tgcttcgcgc gctcgctgag tctccggcga
     9601 cctctcctcc gtgcctcggg tccccgggtg acgtaaaata ccggcgaccc gtaggaaccc
     9661 tgtgagcccg tccacgtcat ccaccatcgc agtcgtcgcc gagaagcccg ccgtcgcccg
     9721 ggatatcgcc cgggtgctcg gggcggaccg ccgcggcgag gggtgcttcc aggggaacgg
     9781 ctatgtggtc acctgggcta tcggccatct ggtgaccctc gccgagccgc acgagatccg
     9841 ccccgagtgg aggaaatggc gccgggacca cctgccgatg ctcccgcgcg actggccgct
     9901 cgtcgtcccc gaggcgacca agcagcagtt cgaggcggtc cggaaggtga tcaacgcgcc
     9961 gtccgtcgag cgggtggtgt gcgctacgga cgccggccgc gagggggagc tgatcttccg
    10021 gtacatctat gaggcggcgg ggtgctccaa gccggtgtcg cgcctctgga tctcgtcgct
    10081 caccccggac gccatccgca aggggttcca gcgcctgcgc gacggccacg actacgaccc
    10141 gctcgccgac gccgcccgcg gccgcagccg cgctgactgg ctggtgggga tgaacctctc
    10201 ccgcgcctat accctggact tcaacgagga cctctccgtc gggcgggtgc agactcccac
    10261 tcttgccatg gtggtggagc gggagctcgc catccgcgcc ttcgttcccg aggactacct
    10321 cgaggtggtg gcgacgttca cccccgatct ggaagctgcg gagccgcgca cctatcgggg
    10381 cacctggttc cgcggcgact cgccgaccgc ggaggcgaag cggctccagt ccgagaaggg
    10441 gaaggagggg gccgagttcg cggaggcggc ggggatcgtc gcccgggcga agagcggcaa
    10501 ggcccaggtg gagtcggtgc gcgccgagac gaagcggatg ccgccgccgc tcctctacga
    10561 cctcacggag ctgcagcggc acgccaaccg actgttcggc tggagcgcca agaagacgct
    10621 ggggatcgcc caggggctct acgagtcgcg caagcttatc agctatccgc ggacggacag
    10681 ccgccacctc tcggcggacg tcgccgcgac cctgccggag atcgtgcgcg cggtctccgg
    10741 gccctaccag gaccgcttcg cccccggcac cggcgagcgc cccctgtcgc gccgcttcgt
    10801 ggacgactcc aaggtcaccg accaccacgc catcctcccc accaccacct cggccgaggg
    10861 gatggacctc tccgccgacg agcgccgcct ctatgacctg atctgccggc gcctgctctc
    10921 ggcctggcac gacgagcacg tctggtcggt gacgacggtg atcacccgca tcacgtccgg
    10981 tgaagtgatc gaccgctacc acagcaccgg caccgccgtg gagcaggtgg ggtggaaggt
    11041 gctcgacctc gacaggaaga aggacaagaa ggccagggag aagaaagagg cgaaggcgga
    11101 ggggggcgag gagccggacg agccggcgga ctccacggac gacgagtccc aggatctgcc
    11161 gccggggctc gtccaggggc agccgcagcg ggtgctcgac gccaaggcgc tcgccaagaa
    11221 gacccggccg cccaagcgct tcaccgaggc gacgctcctg accgccatgg agacggcggg
    11281 caagacgctg gacgacaagg agctgtccga ggcgatgaag gagtccggcc tcggcacgcc
    11341 ggccacccgc gccgagatca tcgagacgct ggtccgccgc gagtacgtcg tccgccaggg
    11401 caagtcgctg gaggccacgg acaaggggat ccgcctgatc caggtggttc atccccacgt
    11461 gaagagcccg gccatgaccg gccagtggga ggcccacctc cagagtatcc agcggggtca
    11521 ggcggatctc gcgacgttca tggagaagat cgaggcctat atcaaggaag tggtggggcc
    11581 ctcacccccc ggccccctct cccgcctccc cgaccccctc cccgggagag ggggggactt
    11641 ttcgggaagg acggcgccgg ataggggggg ctccaggagc ccctcaccca acttcgtgcc
    11701 gcagggggcg ggggtgcctc cccccctggg cgggcgggcg gcagggagcg ggggaggtca
    11761 ggaggggggt tctcctgcct atggccggca gctccggttg gtcgactccg cagcgggagg
    11821 cggccctcgc tcggcacagc cttcccctcc cacccagggc cggcagctcc ggctggatgg
    11881 gttcagtcct ccggcctccc ggcccccctc ccgggagggg gcaagcccct cccctacaca
    11941 gaggttcggg tccggagcga tgccgaacca gagcgcgcct cctcggcagg ctccgcccgc
    12001 ctcgttccag ggggatcgca cccccacgcc gcccgagcgg ctgaccgatc tgctgcgctc
    12061 ggcgttccgt ctccaggagt tcaggccttt ccaggaggcg gtctgccgga cggtcaccgc
    12121 gggccatgac gcgctgctgg tgatgccgac cggtgccggc aagtcgctct gctaccagct
    12181 ccccgggctg gcgcgggccg gtaccacgct ggtggtctcg ccgctcatcg cgctgatgga
    12241 ggaccaagtg gcgaagctcc gggagctagg gctgcgggcg gagcggatcc actcggggcg
    12301 cgaccggggc gcctcgcggc aggtctgcat cgactacctc caggggaagc tcgactatct
    12361 gttcatcgct ccggagcggc tgtcggtgcc gggcttcccc gagatgctcg cgcgccgcaa
    12421 gccggcgctc gtcgcggtgg acgaggcgca ctgcatctcg cactggggac acgacttccg
    12481 gccggactac cggctgctgg ggcagcgcct gcccctgctg cggccggcgc cggtcatcgc
    12541 gctgacggcc acggcgacgc cgctggtgca ggacgacatc gcgacccagc tcggcctcgg
    12601 cggcacggag cgcttcatcc acggcttccg gcgcaccaac atcgccgtcg aggtggcgga
    12661 gctgcggccg tcggcgcggg cggaggcggt gcgccgcgtc ctcgccgagc cgggccgccg
    12721 gccggccatc gtttacgcgc ccacccgcaa ggaggccgac gcgctcggcg agctgctcca
    12781 ggaggacttt ccggccgcca cctaccacgc cggcatgacc acggccgccc gcgaccgcgt
    12841 ccagtcggcg ttcctctccg gcgcgctgga ggtcatcgtc gccaccatcg ccttcggcat
    12901 gggggtggac aagtccaaca tccgtacggt gatccacacc ggcctgccgg ggagcctcga
    12961 gggctactat caggagatcg gccgcgccgg ccgcgacggc ctcccctcgc gcgccatcct
    13021 cctctactcg ttcgtcgacc ggcgcgccca cgagttcttc cacggccgcg actatccgga
    13081 catcgacatc ctgacgcgca tccacaaggc gctgaccgac gagcctcaga cgcccgggag
    13141 gctgcgcaag aagctcaaga tcgacgagga ggaggtcttc gagcgggcgc tggagaagct
    13201 ttggcttcat ggcggcgctc tgattgacgc cgacgaccag atcacccgtg gcggcgacgg
    13261 ctggcggctg ccgtacacgg cccagagcga gcacaagctg gcgcagatcg accagatggt
    13321 gcgcttcgcc gagtcccacg gctgccgcat gctccacctg gtcggccact tcggcgacca
    13381 ggaggactca ggggcgccgt gcggcatgtg cgacgtctgc gccgccgccg agtgcgtggt
    13441 ccgcagcttc cgggaaccca cggtcgccga gggggtggtg ctgcggcgca tcgtcgagac
    13501 cctggcggag cgccgcggcg cctcgaccgg gcagctctac cgcgaggtct gcggcgacgg
    13561 ctccccccat cccatggacc gccgctcctt cgagcacctc ctgggcgggc tggccgccgc
    13621 cggcctggtg cggatgatgg aggactcctt cgagaaggac ggcaagctca tccacttcca
    13681 gcgcgccgcc ctgacgctgg aggggcaccg cgccggcccg gacggcgccg tcggagtcag
    13741 cctggccgac cagccgcctc cgccgccgaa gaagaagcgg tcccgagaga agaaggaatt
    13801 gaagaaggag agggtggggg agaggccggt ccgcgtcagc ccccgggtgg tggagggaga
    13861 ggaggcgggc gaggcgctgc ccgtcttggt cgaggccctg cgcgcctggc gccgcggcga
    13921 ggcccagcgc cggaaggtgc cggcgttccg catcctcacc gaccgggccc tgaccgccct
    13981 cgccgccgcc cgccccgccg acgaggcgga gctgctgggg atcgcgggga tcgggcccac
    14041 catcgtcaag aaatacggac gcgagctgct ggacctgctg cgggccggag gctgacggga
    14101 tagagctgag tagaaaaaaa gatggggaac ccgtctagcg ggctccccga ggaaggcctt
    14161 tgtcgcttcg cccgctcggg gcgttgcgtt tcagggtggt ctcggacccc cggttttcag
    14221 aaaacgtacg aggacaccgg cggcacgacc cggagcgcga acgctcctct gaagatccaa
    14281 ggtgcatgac cctggcctaa gtgggacaaa cttctttaat tttcaatcca ggctgcgctg
    14341 taaccgtttt caagctgaaa gagaggcagc actgagcccg tcttccgggg ccccggtgga
    14401 cggccgagtc ggagcctcag ggggtggtcc gggggaaggc ccaacggctc ccggagccgg
    14461 tcggccaggg agcgggccac gaagccgaag gtgagccggg cgttgatctc gcccaagggg
    14521 ttgaagccgg tgccgccgtc cggggactga tagcgccagg cgtccagtcc gaagggtccg
    14581 gagtagccgg cccggcggag cgccccggcg gcggcttcca gggcctcctc catctgccgg
    14641 ccctcgctgg ccgagaggtt ggggaagccg ccgtcgtagg aggccgtcgt ctcgatcccc
    14701 gtgaactgcc cccggcggtc gaccagcagc cggtggaagc cggccacccg cagccccgag
    14761 gaggtgagca cggccacgca cccgaagtcg tccgtccgct ccatccaggg ttcgaagagc
    14821 agggagccgt gccgcgcgaa gaggttctcg acgcgccgcc gcaccgccgg gtcggagagg
    14881 ctctcgccgg gcctatggat ataccgggac cgccccgccg ccgagagagg ggccttgagg
    14941 acccagctcc tctggaaggt cgtcaggtgg cggtcgagct cgtccggaga ggaaagcatg
    15001 cgggcacccg ggagggcctg gtccaggtcc cgggcgaccg ccaggcagaa cgagcggtga
    15061 tggaccgccg ccaccactcc gggaggggcg ggcggcacgc gccagagcag ctcatggaga
    15121 ggggcgttca gatccggcgg gggcggcggg gtggctggcc ggagcgccgc cacagcagga
    15181 ctctcccccc aggcgagcag ctcgccggcc ggagggagcg acgcgagggg gccgctctca
    15241 aggatcggga ccgggatgcc aggcagcggg gcgagacagg cgggatcgac cggagccggg
    15301 gtccacaggc ggtccccctc gcgggcgaag gcgcggagca gcgtcgccgc gccggccgcg
    15361 gtctccagca cggagctgga gggagagcgc cggggagccg ccagctcctc ctcgaacagg
    15421 aggttgccga cgaggcggcg ggtcacggtt ggctctctgt cagctctcgt acgtggagga
    15481 tctcgctctt cccgatcggc cggatcgcat cagcgcccaa agtcaacagt gccacggtgc
    15541 tgcctcctcc ggtgacgaac tccacttcga aagcagtctc tcgggtatac acgtggagca
    15601 ccgtccctat atcccctcgc tccagcccgt gtgcctcaac gtcctgagtg agtgcgaccg
    15661 tatccagctc ctgaatcacg ttctgagcct ttcgcgaggc gccgggtcac ggttggctct
    15721 cttcgagccg gcggatgaag tcggggtcga ggccggcggc ttcgcggccg gcgcggtgaa
    15781 agcggaggcc cttggccttg gccggacgta gcggccaggt gaggtgggac tggaaggcgt
    15841 cccaggcgga ctcgtcctct cccttgaagc ggtggagcca ggtccagccg aagcggacgt
    15901 gctccacttc gtcgcggtgg acctcgtcga tcaccgcggc ggtcttgggg tcgtccgccc
    15961 ggcgcgccgc ctcggagtac tccaccgtgt ggtcgaggtt ggcgttctcg aaggtcagcg
    16021 acatggcgca gacgaagtcc aggggggatt ggatcgccgg cgtcttgctc cagaaatagc
    16081 cgttgaccgg gaaatcgccg aggtcggccc cccaggcccg gaggcgggcc cggtacatcc
    16141 gggtgtgccg ctgctcgtcc tggaggatgt ggaggaggcc gcggcggaac ccagcggggg
    16201 cttcggggaa cgccagcagc gcccaggcga agagctccgc cgcctgcaat tcatggttgg
    16261 ccagggcatg gaggatgcgt ggccgctgcg ccgggtccgg catcccctcg atggacggta
    16321 cgttgacccg ggacggcggc ggcagccgga ggttggccgg ccggcccggc cccgccgccc
    16381 ggtacgccgg gccggggtcc tcgtccgtca gcggtcccgc gggaggagcg agcttgacgt
    16441 ccaggtcctc ggtctcgagg acggtccggg cgaagtcgcg gatctccatc gcctacgcgg
    16501 agaccaggcg cccggcggcg acggcggcaa ccgcctccag cagccggtcg atctcctcgc
    16561 gggtgttgta catcatgacg ccggtacgga cgacgccccc cttctcctgg agccccagca
    16621 cctcgatggc gcgggcggcg tagaagtggc cgtcccagac ctgcagcccc tgctccccga
    16681 gccgcgccgc cacctccgag gaggggacgc cgtcgacggt gatggagacc gtgggcgccc
    16741 ggcgcggcga ggtgaagtcc ggcccccaga cgcggacgcc ggggatctgg cggacgttgt
    16801 cgtggtagta ggcagcgacc gaatgctccc aggcggcgat tccttccatg gccgagacca
    16861 gccgttcccg cagagtctgc ccctctcccc acgaggcgat gtagtcgatc gccgccttga
    16921 cccccgcgat ggccgcgtgg ttgagggtgc ccgtctcgat gcgggagggg gcctcgtcct
    16981 cctgggtgct gagcttgtcg gtgcgcagcc ggtccagcag gccgggccgg cagtagagga
    17041 ggccgacgtg ggggccgtag aacttgtagg ccgagcagag caggaagtcg gggtcgagcg
    17101 ccgccacgtc gaccgaaaag tgggcggcat agtggacggc gtcgatcagc agccaggcgc
    17161 cgacctctct ggagagtcgg cgcgccagga ccacgtcgtt gaccgtgccc agggcgttcg
    17221 aggcgccgcc gagggcgacc aggcgcgtcc tcccgttgat ctgccgcgcc atgtcgtccg
    17281 ggtccagcgt gccgtcgggg cgcagggcca cctcgcggac ggtgatcccc cgctcgtggc
    17341 cgcactcctt cagatgcagc caggggccgc ggttcgactc gtggtcgagc tgggtgatga
    17401 cgatctcgtc gccgggcttc atctcccgcg ccagggcgtg gctgagggcg aaggtcagcg
    17461 tggtcatgtt ctggccgaag gagatctcgc gccagctcgg ggctccgagg aaggccgcca
    17521 ccgcctcgcg ggtctcgatc agcagccggt ccgtctcccg cgaggtggga aagaacccgt
    17581 gcaggttcgc gttcgacgtg gcgtagtagc cggagatggc gtcgatgacc tgccgcgcca
    17641 cctgggttcc ggcggggccg tccagaaagg ccaggggccg gccctcgaag ctgcgctcga
    17701 gggcggggaa gtcccgccgg catttgaggc aatcctgttc ggtaaaggcc aagagtttcc
    17761 ctcccgggtc caccctactg gaagtacccg aagacgtcca gcaccatgtg ggtgctcccg
    17821 gacgccatgt cgcagcggac gcccacgctg ccgtccgcgc ccaggccgac gatcgcgttg
    17881 ttggcccggg tgcggtgggt cctgaagttg atggtgctgg tgagcggcgt cccctggtcc
    17941 ccgggataca gccgcaggcc cccccgcgtc gtgggctgga ccacggtgac gttgaccgcc
    18001 acggccgacg cctccgccgg cacgccgcac cggcccgcca tctggaaccg ccggtcggta
    18061 ttcgccgcca gcgccgggcc gtccgcgtcg cgggtgtcgg cgatccggca gggggtgacg
    18121 gtgtggagct cggtggcggg gggcggcggc gcgctgtggc aggcggccga gccgaaatcg
    18181 gtgaaggtgg accccgccgc cggacggaac cgccagtcgt agccggtggg gcggagcgtc
    18241 agctccagca cgccgaaggc cgcggcgctc cgtgcctcgc tgttggcgcg gaccaccggg
    18301 aacgaggtca ggttcttacc gcccgttccc gccacgaact ggcggatgcc ccggccgagg
    18361 tcggcgttgc cgtcgacatc ctggggggcg aagcgctcgt agatgtggtc gtggccggtc
    18421 aggatcacgt ccgctccggc cgcgtgcagg gcgcgccaga aggcgtccgt ggaggcgtcg
    18481 ttgccgtgcg gacccgagga gaagcggggg tggtgccagg ccgcgagggt acagcggccc
    18541 gggttcgccg cgaggtcggc ggccagccac tgctcctgag gggagcccac cccgcagccg
    18601 cccacctggg cgcagtcgga gttgaggacg atcacgtgcc agctcccgac gtcgaagctg
    18661 tagtacccct ttgcaggatc ccccgccgcc gcgccgaagt aggagtagta gccggcggcg
    18721 ccgggggtca agtattcgtg attgccgacc accggccggg tgatcgcctt cagccgcccc
    18781 caggtggggt cgtaggaggc catgaacttg gcggcggtac cgttttcgta ttgggtgtcg
    18841 cccagggcga ggacggcggc aaggtcccta cccaccagca ggtccgaggt ggcgcgcatg
    18901 cggcaggcgg tcgccgtgcc gttcgtggcg ttgaaagaag gatcgcccgg atcacaggcg
    18961 atgtcgccaa ccgcggcgat gaccggaggc gggggatcgg cggcgcggac cggggcggcc
    19021 cagaagacga gcccgaggac aacgagatag aaggcgcggc gtggcatggc ggaccggcag
    19081 gggggcgggc cgggcggccc gccccggtca gagtttagta ccggatgatc gccgacagca
    19141 cgaacgagcg cggcgtggaa taggtggtgg tggtgccgaa atcggggttc ggcgtcatgg
    19201 cccggccaag gagcaggcgg tcatccacgg cgagctgggc ctgctcgtcg aagacgttgg
    19261 tcatccgggc ctcgagctcc aggccgacga tggagagcgg gaactcgtac gaggccagga
    19321 ggtcggtgtt gaaccaggag tccatgcggc gcgagcccgc gcgctcgagg taggcgatgt
    19381 gcgaagcgct ggagaccgtg gcggccggca ccgaccgggc ctcccaggcg gcgccgctct
    19441 gatagcggag gtaggtgccc acccgcaggc gctccaccgg ctccaccgtg gcgaagacct
    19501 tggccacgtg ggtacggtca ccgcgcatca ggccgtcgcg gttgtcggtg aggagcacgc
    19561 ccgggccgtc gccgaggaac gaggagttgt agaacggcga gtcgccgctg ctgaagtcga
    19621 tatcccagtt gccctcgagg cggctccagg tgtagctcgc ctccagcgcc aggccgagcc
    19681 agcggccgtc gatcggccgg cgcgacacct gcagggtggc ggcggtgtac tcgcgatagg
    19741 cgtcggggag ctgggcgacg cggaacgggc cgttgcccag gccgtcgcgg ctgatgtcct
    19801 ccatgatgtc gccgacctcg cggtacatgc cccagatctc ggcggcccag ccgcggccga
    19861 acggccgggc gtagccggcg acgaactcgt cggtgaactg gggatcgagt ccgggatcga
    19921 tcgtcttgct ctgcgtgttg gcctgcgccg tctcgctgat gaagttgccg gcggcgtcga
    19981 accgcgcagt ggtcgagaag atgcgggtcg gcgacgcggc gcgcaccagc gacttgttct
    20041 cggtgttgaa gtagcggccg tagttgaagt agagcttgtc gccccgctcc aggctcggca
    20101 cgaaggagac gccgaggcgc ggctggatct gctggtccca atcgaaggtg aggagcttca
    20161 cctcgcgttt ggtgccggga gtcgagccca gcgcctcgcc atagtaggtg tcgcggttga
    20221 ccagcacgcc ggcgacgatc gtcgtccgct ggccgagggt gaactggtcc tgggcgaaga
    20281 ggcccagcgc ctcgccgcgg ccggtgtgag gcggctgctg cgagacgtag gtggcgctga
    20341 actgctgggt cgcggcgttc caggtgatca cgccccagcc gttggcgcgg cggttcaggg
    20401 tctcctcgtt ctcgtcgtag gtgacccccg cccgcaggtc gtggctgacg ccgaaccagt
    20461 cctggaagct ctggaacgtc gcccgggcct cgttgcgctc gaagttctgg tcgttgatcg
    20521 ccaggtcggt gccgccgacg agctggccgg tgcggttggc gccgccgggc cgccggtcgt
    20581 cggtggaggt gaagcggccc accagatcgg ggcgcgcggc gttgaacgcc ggccggtagc
    20641 cgaggtcggt cgcgggatcg acggagttct cttccttgtt gtagttgagc ttgagctcga
    20701 ggaagctgtt ctccgtggtg ttccaggtcc agcccagggt cgccagcagg tagtcggtga
    20761 tcgtggtgcg ccggaccgtc gggctgacgt tgacgtcgag gccctcgttc tcgctggaga
    20821 cctcacgcga gcggccggag atgttcaggt agtggttctg caccggctgg gaggtgaact
    20881 tcaagaagta ctcgtcggtg ttcacctcga ggtccgggat gctgccgaac tggttcgtcc
    20941 gctcggtggt gctctggcgc gggaagctcg ccgagccgta gtaccagagg tggtcgcgta
    21001 gcagcgggcc gcccagggag gcggcgacgc cgctgcggtc gggagtgttg gagacgttgg
    21061 ggttctcgct gtcgctgacg aagtcgttgg gctggtactc cagccgcgcc tcgcccgcca
    21121 gctcgttggt gcccgacttg gtgacggcgt tgaccaccat gccgccggtg cggccgaact
    21181 cggcggtgat gccgccgcgc ttgatcgcca cctcctcgaa gtccagctcc gtgatgttgg
    21241 gcaggatgtc gccgaagtgg gggttggtga tgttgatgcc gtcgatcagg aagttgttgt
    21301 ccatccgcga gccgccggcg ttgggcgtca ggcggtcgtt ctcgctgacg ccgggggcga
    21361 gctggaacag ccccttgtag gtgcgcggca ccggcaggtt ctcgatgaac tcgtgggtgt
    21421 agttcacctg gatctcggtg gagcggacgt ccaccaccgg cgccacggcg ctcaccgtga
    21481 tctcgtcctg caccgtcgcc gggctcaggg tgaggccgac ttgggtgtcc cgggcgacgg
    21541 cgacgatcac ctcgcgccgg gtgttgccca tgcccgacag ctcggcgctg accgtgtagg
    21601 ggccgggggg caggcgcagg atggagtagg agccgtcgct cgccgaggtg gcggtgcggc
    21661 cgccggggag cagggggccg ctggcggtca ccaccacgcc gggcagcggc gagccgtcgc
    21721 ccgactggac cacgccggag atggagccgg tctcctgggc gagcacgggg accgcgagca
    21781 gcaggatgaa gagacagacg agcagccgaa aactggcgcg ggatctcacg gacatgggga
    21841 gcctcctgtc gtgaagggcc ggagccgccc cgccggcggc ccacgggccc ggatctcatc
    21901 aaagaatcac aggaggcgat cctaccacct tctaccagcc ctggcgggcg cgcagatcgg
    21961 tgatctggcg cacgtggttg gcgccgtggc cggcgtagag gccgagcagg tcgtccaagg
    22021 tgatgtcacc ccgctccgga tggaacgccg tgcgcggcca ggcctcctcg gggacggcgc
    22081 gcagcagcgc ggtacagcgc tggtgcaggc cgcgcacgat ggcgagcgac ggctccagcg
    22141 gcagcgccga gtccgccagc cctgcccact cgtcctggtc gtaggtcttg agcgtgggat
    22201 ggtcctcggt gaggatgagc ttcatgcggg tgacggcgtt ggcgtgggag tcggccaggt
    22261 gatggaccac ctggcggacg gtccagcccc cctcccggta gggggtgtcg agctgctcgt
    22321 cgtcgaggcc gcggacggcc tcctccagga ggcgcggcag ctcctcgatc cggtcgatct
    22381 gcttttgacg ctcttcggcg gtagccatcg gggaagtctc ctctttctga tctataggga
    22441 gggtcaggcg gcggcgcggt cccggccgag gaccccggcc agcgccgcgg cgaagcgtcc
    22501 gggggctagc gggtcctggg cgaagaacag cgagggctcc agcatctcca gctccatcag
    22561 gtggaagccg cgcctgccgc tgaggacgca gccgtcgacc cgcgcgtaga gccaggggcc
    22621 cgcgaccgcg gcgaggatcc gctccgcccg ctccaggacc gcggccggcg ggtcggccgg
    22681 cgccgcggag ccgccatggt cgatctgcac ccggaagtcg ccggacttgg ggcgcttgat
    22741 cacggcgtgg ctga
//
LOCUS       DASPJI010000458         4212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_661416, whole genome shotgun sequence.
ACCESSION   DASPJI010000458 DASPJI010000000
VERSION     DASPJI010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661416"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VEL74_12480"
     CDS             complement(<1..673)
                     /locus_tag="VEL74_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63392.1"
                     /translation="MGVRKFETMSPQVWLQAVAVMVLAVLLYSGLRSELPVDDVLRFT
                     PAVAAGSYEWDSAHLLMQPSAVLWHRWLGFGAPARTSQERFNAFCGGLSLAILYLLLL
                     RLGVDLPRRILLTALGALAFNQILLGTSGHFKLAVLPFLTLSLYHGVIWERSVRAEPG
                     GGDGRLAASAIFLGVATAFLVNSILVAPFLGLAAAAISLRSGGGLRRAFKRLLGVGAL
                     SGGTAL"
     gene            1260..1787
                     /locus_tag="VEL74_12485"
     CDS             1260..1787
                     /locus_tag="VEL74_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63393.1"
                     /translation="MRNTGTEQPFDKVVWLAGYLLAHQAGITAEGEAAVPRALVVTRD
                     QPDDAQLLVFEAASYQEAVAQGRSLLADQKDGLRCWAFAYDAGRLSGGLTDGDALVVE
                     ANVEDGDRPLSFLQRYQPKSDSAPFRLIGGVEMAGAWSRPAADGAREPAAWDSLLREG
                     IQSHPLAGEAWTLYL"
     gene            complement(1920..2393)
                     /locus_tag="VEL74_12490"
     CDS             complement(1920..2393)
                     /locus_tag="VEL74_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63394.1"
                     /translation="MAWGKKKSGGGGGVAWLALIVALAALFLAWKAYERTGGDLDGIL
                     RLPVGEMEVGEGEGDETAVERQAGLAQARARLLARRTEVAARRNLRQAQEEVAEVRDQ
                     LQGAYKGAGDQAREGWRELDADLERLQGQLRDGGSRAVETLDSVVDKMRRIGEDG"
     gene            2464..3039
                     /locus_tag="VEL74_12495"
     CDS             2464..3039
                     /locus_tag="VEL74_12495"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HYG63395.1"
                     /translation="MENLWEGVRWALGIDVAPQALNTLQICLRAVLVYLAGWAILRAG
                     GNRFLGRETAFDIVLGFVLGSTMSRAINGSGPILLTLVASALLVALHQLLAWASFRSH
                     RFSVLMKGSPEPLIRDGKVIPESLRKHSLSEGDLEEALRLHAHLAGPSEVEEARYERN
                     GDISIVKKKDGPRVVEVRVEQGVQTVRIELG"
     gene            3177..>4212
                     /gene="htpG"
                     /locus_tag="VEL74_12500"
     CDS             3177..>4212
                     /gene="htpG"
                     /locus_tag="VEL74_12500"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HYG63396.1"
                     /translation="MATQVEEPQVEVKQFQAETKQLLDLMIHSLYSNKEIFLRELISN
                     ASDALDRLRFEALTRPELLEGDEKLQIRLEADREKRTLTIHDNGIGMSRQEVIENIGT
                     IAKSGTRELVERLKSGESKQSIAELIGQFGVGFYSSFMAADKVELVTRRAGDTEATHW
                     ESTGDGTYRIVPAEKPGRGTSITLHLKPEDRDNGIDEFSDRWVVSRIVRRYSDFVGYP
                     IVLTAEKDPEIEDLAKEKETGEKVKMPLEEKVLNSMQPIWTKSQSEVTQEEYKDFYRH
                     ISHDFSEPLKVIHYKAEGRIEYQALLFVPSQAPYDLYYVASKPGLHLYVKRVQIMEKC
                     EDLLPQYLRFI"
BASE COUNT          772 a         1422 c         1401 g          617 t
ORIGIN      
        1 caagggcggt gcccccggag agggcgccca cacccaggag gcgtttgaag gcgcgccgca
       61 ggccgccgcc ggaacgcagg gagatggccg cggccgccag cccgaggaac ggcgccacca
      121 ggatgctgtt gaccaggaag gcggtggcga cccccaggaa gatcgccgac gccgccagcc
      181 gcccgtctcc ccctcccggc tccgccctga cgctccgctc ccagatcacg ccgtggtaaa
      241 gggaaagcgt gaggaacggc aggaccgcca gcttgaagtg acccgaagtc cccagcagga
      301 tctggttgaa cgccagggcc ccgagggcgg tcagcaggat acgccgcggc aggtccaccc
      361 ccaggcgcag cagcaggagg tagaggatgg cgagcgacag gccgccgcaa aaggcgttga
      421 agcgctcctg gctggtccgc gccggcgcgc cgaaccccag ccagcggtgc cagagcacgg
      481 ccgatggctg catcagcaga tgggccgaat cccactcgta ggagccggcc gcgaccgcgg
      541 gggtgaaacg cagcacgtca tcgaccggca gctcggagcg tagaccgctg tagagaagca
      601 ccgccagcac catgacggcg acggcctgca gccagacctg cggggacatt gtctcgaatt
      661 tccggacacc catacggcgg acctcccttg gcggggtcgg acgtgagacg aggctggacc
      721 ttgttaaaaa gctgcaggaa tcgtgccttt attttagctt gtcgtgtata tatcaataag
      781 gtggggcgcg ctcaggggcg gcgcggtgcg gcagggattt ttagagtgtg agagacggga
      841 atggaaggta cgaattacat cgcgaacggt gaaaaagaaa aggccgccaa gattcacacc
      901 ccggcggccc aaaggatcac tctttcgagt tgaagatcag taccggctct ccgcacactg
      961 gagggcggca ttggcgtcgc tctccagtgt ccggccgccg gtgccgaaat acgcggccaa
     1021 agacatagaa cccctgtggg gaaagacgtt gctttcccca aaaatcccga ggagccggaa
     1081 ggctcacggg ttgaagaacc ccttcgaaga cgaaagcttc gagccacggc ccagatagtt
     1141 gagtgctcta ctctcctggg tgctcctccc ccgtcaggcg tgacacgttc cgtgcgattt
     1201 gttggagggc gggtgaaatg tcctgcctcc ggcggtcact ccggagagga ggtgaccctt
     1261 tgaggaacac gggaacggag caaccctttg acaaggtcgt ctggctcgcc ggctacctgc
     1321 tcgctcacca ggcgggcatc accgcggaag gtgaagccgc ggtcccccgc gccctggtgg
     1381 tgaccaggga tcaacccgat gatgcccagt tgctggtgtt cgaggcggca tcttaccagg
     1441 aagctgtggc gcagggacgg agtctcctcg ccgatcagaa ggacgggctc cgctgctggg
     1501 ccttcgccta cgacgccgga cggctctccg gcggcctgac ggacggcgac gccctggtgg
     1561 tcgaggcgaa cgtcgaggat ggcgacaggc cgctgtcgtt ccttcagcgc taccagccga
     1621 agagcgactc ggcccccttc cgtctcatcg gcggcgtgga gatggccgga gcgtggtccc
     1681 ggcccgcggc ggacggagcc cgggagccgg ccgcgtggga cagcctgctg cgggagggca
     1741 tccagagcca ccccctggcc ggcgaggcct ggacccttta tctataagga aggagcctgc
     1801 agcgcccgcg cgaggagcgc ctgggtggcg gccgagggga gacagggcct cccatagcgc
     1861 gcgggggagg cggcgcggat cacctcgtgc gcccgcgtta tcggatcggg cggctccact
     1921 cagccgtcct cgccgatccg ccgcatcttg tcgaccaccg agtccagggt ctccaccgcc
     1981 cgggagccgc cgtcccggag ctggccctgg aggcgctcga ggtcggcgtc cagctcgcgc
     2041 cacccctcgc gggcctggtc gccggctccc ttgtaggcgc cctggagctg gtcccggacc
     2101 tccgccacct cctcctgtgc ctgccgcagg ttgcggcggg ccgccacctc ggtccggcgg
     2161 gcgaggagcc gggcgcgcgc ctgcgccagg ccggcctggc gctcgactgc cgtttcatcc
     2221 ccctcccctt cccctacctc catctcgccg acgggcagcc gcaggatccc gtcgaggtcg
     2281 ccgccggtgc gctcgtacgc cttccaggcc aggaacagcg ccgccagcgc cacgatcagg
     2341 gccagccagg ccacacctcc tccgccgccg ctcttcttct tgccccacgc catccgcagt
     2401 cctccttgga gggggatctt acaggctcgg cggttgcagg ggagccgcct caggaggtgc
     2461 cgaatggaga acctgtggga aggagtccgg tgggcgctgg ggatcgacgt ggctccccag
     2521 gccctcaaca cgctgcagat ctgcctgcgg gcggtcctcg tctacctggc gggctgggcc
     2581 atcctgcggg ccggggggaa ccgcttcctc ggccgggaga ccgccttcga catcgtcctc
     2641 gggttcgtcc tcggctccac catgagccgc gccatcaacg gctcgggccc catcctcctg
     2701 accctcgtcg cctccgccct gctcgtcgcc ctgcaccagc tcctcgcctg ggccagcttc
     2761 cggtcccacc gcttcagcgt gctgatgaag gggagccccg agccgctgat ccgcgacggc
     2821 aaggtgatcc cggagagcct gcgcaagcac agcctcagcg agggggacct cgaggaggcg
     2881 ctacgcctcc acgcccacct cgccggcccc tccgaggtcg aggaggcgcg ctacgagcgc
     2941 aacggagaca tcagcatcgt gaagaagaag gacggcccac gggtggtgga ggtgcgggtc
     3001 gagcaggggg tgcagacggt ccggatcgag ctcgggtgac acctggaata gagggggcag
     3061 gaagggggtt gaaaaactgc gtgcgaggca ctaaactatt gcacaccgca ggatgatgct
     3121 cctccactca actttcggag gggctcatag agtcatcaag gagcaaggag aaggccatgg
     3181 ccacccaagt cgaagaacct caagtggaag tcaagcagtt ccaggcggag accaagcagc
     3241 ttctggacct gatgatccac tcgctgtact ccaacaagga gatcttcctg cgcgagctga
     3301 tctccaacgc ctcggacgct ctcgaccgcc tgcgcttcga agcgctgacc cggccggagc
     3361 tcctggaagg ggacgagaaa ctgcagatcc gtctcgaggc ggaccgcgag aagcggaccc
     3421 tcaccatcca tgacaacggc atcggcatga gccggcagga ggtcatcgag aacatcggga
     3481 ccatcgccaa gtccggcacc cgcgagctcg tcgagcgcct caagtccggc gagagcaagc
     3541 agagcatcgc cgagctcatc ggccagttcg gcgtcggctt ctactcctcc ttcatggcag
     3601 ccgacaaggt ggagctggtg acccgccggg ccggcgacac cgaggcgacc cattgggaat
     3661 cgaccggcga cggcacctac cgtatcgtgc cggccgagaa gcccggccgc ggcacctcca
     3721 tcaccctcca cctgaagccc gaggaccgcg acaacggcat cgacgagttc tccgaccgct
     3781 gggtggtcag ccgcatcgtc cggcgctact cggacttcgt cggctacccg atcgtgctga
     3841 ccgccgagaa ggaccccgag atcgaggacc tggcgaagga gaaggagacc ggcgagaagg
     3901 tcaagatgcc gctcgaggag aaggtcctca actccatgca gccgatctgg accaagtccc
     3961 agtcggaggt cacgcaggag gagtacaagg acttctaccg gcacatctcg cacgacttca
     4021 gcgagccgct caaggtcata cactacaagg ccgaggggcg catcgagtac caggcgctgc
     4081 tcttcgtgcc gtcgcaggcg ccgtacgacc tctactacgt cgcctcgaag ccgggcctcc
     4141 acctgtacgt gaagcgcgtg cagatcatgg agaagtgcga ggacctgctg ccccagtacc
     4201 tgcgcttcat cc
//
LOCUS       DASPJI010000459         4324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_539043, whole genome shotgun sequence.
ACCESSION   DASPJI010000459 DASPJI010000000
VERSION     DASPJI010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539043"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VEL74_12505"
     CDS             <1..312
                     /locus_tag="VEL74_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63397.1"
                     /translation="RVARLIGGHEQPPFPGDPYADDLALLNDADALSFFSLNSPGYMD
                     YFGPAATRRKVAFTLARLRPAARRRLAHMRLRPDVAALVVEHELLWDLPGMPLREAMA
                     S"
     gene            309..1445
                     /locus_tag="VEL74_12510"
     CDS             309..1445
                     /locus_tag="VEL74_12510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG63398.1"
                     /translation="MKLVVFGLTLSSSWGNGHATLWRGLCRALARMGHRVVFFERDVP
                     YYAVHRDLRELPGTDLVLYPDWESVLPRARREVDGADAAMVTSYCVDGVPATDLVLSA
                     QSSGPGQSVFYDLDTPITLERLRAGQPVDYLGPDGLAGFDLVLSYTGGQALTELQERL
                     GARRVAPLYGSVDPEVHRPAEPRDVYRGDLSYIGTYAADRQEVLERLFLEPARRLPDK
                     RFVLAGAQYPADFPWGSNIWFVQHLPPPDHPAFFASSPLTLNVTRAAMATMGYCPSGR
                     LFEAAACGTAVLSDTWEGLDEFFEPGREILLATTTEEAMEALSLSPEEIARIAEAARE
                     RTLAEHTAERRARELLDLLGSTDEPEGSLLSIPGLSPQDVGGAA"
     gene            1475..2272
                     /locus_tag="VEL74_12515"
     CDS             1475..2272
                     /locus_tag="VEL74_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HYG63399.1"
                     /translation="MGSRIQPLAFSKELLPVGSRVESGTERPKAVSEYLIERMILAGA
                     DKICVAISPGKSDIIEYYGGSIGPARICYVVQPQPRGLCDAIFQALPVIAPDDQVLVG
                     LPDTVWFPEDGLRRLPAGALSFLLFPVERPELFDAVVTDAAGRVLEIQVKSQAARSHW
                     IWGAFRMPASVLHDLHELWRERDGRDAYIGTLVNAWLERGGEAFGVPAGESYVDVGTL
                     HGYREAIQLLGSRPAPAQPSEARPAAAERIGSELPPVPILARGTGSP"
     gene            2565..3449
                     /locus_tag="VEL74_12520"
     CDS             2565..3449
                     /locus_tag="VEL74_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HYG63400.1"
                     /translation="MIEAVMLWNEPNNMSHWDFEIDAGWKIFAEMAKLAGAAVRSESR
                     DLPRVLGGISPIDAKFMNNMADQGVLDHVDVVAVHGFPLDWNHWQIHEWPDRLAEIQA
                     VTTLPVWVSEVGVSTFGAEEVQDFGLRRTAELLLGRAPRIHWYSLYDLPKAWPATTRH
                     REAEGSSYYRHFYMGLLREDGTPKIAAAQFPEYTPEMGICQWFHFEDHRLDDAVRWLR
                     KLGVKKLRTGLSWADSHRPNWEPWFDRQMKALEEFDVTVTYCFTPESCGVRPHHTSPP
                     TCVEEFADFCARMTRRYA"
BASE COUNT          621 a         1610 c         1441 g          652 t
ORIGIN      
        1 cgcgtcgccc ggctgatcgg cggccacgag cagccgccgt tccccggcga tccgtacgcc
       61 gacgacctgg cgctgctcaa cgacgccgac gccctgtcct tcttctcgct caacagcccg
      121 ggctacatgg actacttcgg cccggccgcg acccgccgca aggtggcctt caccctggcg
      181 cggctgcggc cggcggcgcg ccgccgcctg gcccacatgc gcctgcgccc cgacgtggcc
      241 gccctggtgg tcgagcacga gctcctctgg gacctccccg gcatgcccct gcgggaggcc
      301 atggcttcat gaagctggtg gtcttcgggc tgaccctcag ctcgtcctgg gggaacggcc
      361 acgccacgct ctggcgcggc ctctgccgtg ccctggcgcg gatgggccac cgcgtcgtct
      421 tcttcgagcg cgacgtcccc tactacgccg tgcaccgcga cctccgcgag ctgccgggca
      481 ccgatctggt cctctacccg gactgggagt cggtgctccc ccgggcgcgg cgcgaggtgg
      541 acggcgccga cgccgccatg gtcacctcct actgcgtgga cggcgtgccc gccaccgacc
      601 tggtgctctc ggcccagtca tcggggccgg gccagtccgt cttctacgac ctggacactc
      661 ccatcaccct ggaacgcctg cgcgccggcc agccggtgga ctacctgggg ccggacggcc
      721 tcgccggctt cgacctggtg ctctcctaca ccggcggcca ggcgctgacc gagctccagg
      781 agcggctggg agctcggcgc gtcgccccgc tctacggcag cgtcgacccc gaggtgcacc
      841 gcccggccga gccgcgggac gtctatcgcg gcgacctctc ctacatcggc acctatgccg
      901 ccgaccgcca ggaggtcctc gagcggctct tcctcgagcc ggcccgccgc ctgccggaca
      961 agcgcttcgt cctcgccggc gcccagtacc cggcggactt cccctggggc tccaacatct
     1021 ggttcgtgca acacctgccg ccgcccgacc acccggcgtt cttcgcctcg tcgccgttga
     1081 ccctcaacgt cacccgggcg gccatggcaa ccatgggcta ctgcccgtcg gggcgcctct
     1141 tcgaggcggc ggcctgcggc accgcggtcc tctccgacac ctgggagggg ctggacgagt
     1201 tcttcgagcc cgggcgcgag atcctcctcg ccaccaccac cgaggaggcg atggaggcgc
     1261 tgagcctctc gccggaggag atcgcccgca tcgccgaggc cgcccgcgag cgcaccctcg
     1321 ccgagcacac ggcggagcgg cgggcgcggg agctgctgga tctgctgggg agcacggacg
     1381 agccggaggg ctccctcctc tccatcccgg gcctctcgcc ccaggacgtc ggaggcgcgg
     1441 cttgagcctc tgggggatcg tgccggcggc cggcatgggg agccgcatcc agcccctggc
     1501 cttctccaag gagctgctgc cggtgggcag ccgtgtcgag agcggcaccg agcgccccaa
     1561 ggcggtctcc gagtacctga tcgagcggat gatcctcgcc ggcgccgaca agatctgcgt
     1621 cgccatctcg ccgggcaagt cggacatcat cgagtactac ggcggcagca tcggcccggc
     1681 ccgcatctgc tacgtggtgc agccgcagcc gcgcggcctc tgcgacgcca tcttccaggc
     1741 gctgccggtg atcgccccgg acgaccaggt gctggtgggg ctgccggaca ccgtctggtt
     1801 ccccgaggac ggcctgcgcc gccttcccgc cggcgccctc tccttcctgc tcttcccggt
     1861 cgagcgcccg gagctgttcg acgccgtggt gaccgacgcc gccggccggg tgctggagat
     1921 ccaggtcaag agccaggcgg cgcgctcgca ctggatctgg ggggccttcc gcatgcccgc
     1981 ctccgtcctg catgatctcc acgagctctg gcgcgagcgg gacggccggg acgcctacat
     2041 cggcaccctg gtcaacgcct ggctggagcg cggcggcgag gccttcggcg tccccgccgg
     2101 cgagtcctac gtcgacgtgg ggacgctcca cggctaccgc gaggcgatcc agctcctcgg
     2161 ctcccggccc gcgccggccc agccctccga ggcgaggccg gcggcggccg agcggatagg
     2221 atcggagcta ccgccggtgc cgatcctggc ccgcgggacc ggatctccct agagggtgtc
     2281 tcgaaagggg agcgtctacg cgtggagtcc tcaccccccg gccccctctc ccatctccca
     2341 accgcccccc gccgggagag ggggagaaaa ataagagatg atgcgggtct ccccctctcc
     2401 cggggatcgg gtgggcggga gatgggagag ggggccgggg ggtgaggact cgcgcctcgc
     2461 tggtctggac ttctgaaaca ccctctgaga cgagaaggac ccgctttttt cccatagacc
     2521 tcaccggctc ccccccgagc cgacgaccgt tgagaggtgt gcgcgtgatc gaagccgtga
     2581 tgctgtggaa cgagcccaac aacatgtccc actgggactt cgagatcgac gccggctgga
     2641 agatcttcgc cgagatggcg aagctggccg gcgcggcggt gcggtccgag agccgcgacc
     2701 tcccgcgggt gctgggcggc atctccccca tcgacgccaa gttcatgaac aacatggcgg
     2761 accagggggt gctggaccat gtcgacgtcg tcgccgtcca cggcttcccc ctcgactgga
     2821 accactggca gatccacgag tggccggacc ggctggcgga gatccaggcc gtcacgacgc
     2881 tgccggtttg ggtctccgag gtgggggtct cgaccttcgg cgccgaagag gtgcaggact
     2941 tcggcctgcg ccgtacggcg gagctgctcc tgggcagggc gccgcgcatc cactggtaca
     3001 gcctctacga cctgccgaag gcgtggccgg ccaccacccg ccaccgcgag gccgagggat
     3061 cctcctacta ccgtcatttc tacatggggt tgctgcgcga ggacggcacg ccgaagatcg
     3121 ccgccgccca gttccccgag tacacgccgg agatgggcat ctgccagtgg ttccacttcg
     3181 aggaccaccg gctggacgac gccgtccgct ggctgcgcaa gctcggggtg aagaagctgc
     3241 gcaccggcct ctcctgggcg gacagccacc gtcccaactg ggagccctgg ttcgaccggc
     3301 agatgaaggc gctcgaagag ttcgacgtca ccgtcaccta ctgcttcacc cccgaatcct
     3361 gcggcgtccg cccgcaccac accagccccc ccacctgcgt cgaggagttc gccgacttct
     3421 gcgcccggat gacgcggcgg tacgcctgag ccgcctcgcg cggccccatg tacgtcaatt
     3481 gtcaaagaac cctagagaga ggattgggct gctcccattc ctctcctcag cggcgagata
     3541 ctaccacaaa atccctctct attgacttgc ttgacagtct cgtgctccag tctctatgct
     3601 tgcgcagcgt tctcctcagc gtctcggatt caatcccagc gttgaggaca gcggcagaac
     3661 gatgcggatc gttcccgaac gttccggaat ccatcccggc cgcgcaggga agcggcggat
     3721 cgccgaggat cgttcccgaa cgctccggaa ccttcccgga tggcgcggga cgtcggcgag
     3781 ctgtcaagga gtggtcccga aggctccgga ttcatcccga agcaccggga cggcacgggt
     3841 tcattgacaa ctgaatcgtg agggaggcgc cgccgtgtcc aaggccgcgc cttctgagga
     3901 ttcctccgtc cgacaggagg agcgcggcca ggcggaggcc cccggagggg cctccgccgc
     3961 cgcgtgtctt cttgagccgg gcgggagggg gcttcctccc cccctgggcg gtgggcggga
     4021 gggagcgggg gaggaccgag gtggggggtt ttctttttgg gacgggcgga gctccgcgcc
     4081 agccccggcg aaaagaagaa aaagaaactg cccccctcct gacctccccc actccctacc
     4141 gcccaccctc ccaggggggg aggcaccacc acccggtggc gcccccctat ctaaaggatg
     4201 tgaaggatgt cctactccgg tccgcccacc ccgtccgccg ccacccgctc gtcgaggatg
     4261 gcgccgcacc gctccagggc cacggacccg gccgcctcca gcacgaaggc gagcgactcc
     4321 ggcg
//
LOCUS       DASPJI010000460        12367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_86676, whole genome shotgun sequence.
ACCESSION   DASPJI010000460 DASPJI010000000
VERSION     DASPJI010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12367)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12367
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86676"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VEL74_12525"
     CDS             complement(<1..684)
                     /locus_tag="VEL74_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HYG63401.1"
                     /translation="MSGLAVALLSFWWLRGAGQPPAEPSRPAAPPATQAAAPGPERLR
                     AQVVSIRPHDPQSYTQGLLWHDGSLYESAGHYGQSSLRQVDPATGEVRRRLDLPPQYF
                     AEGLTLVGDRLIQLTWHEGTALVYRLSDFGKLKELTYTGEGWGLCHDGKRLVMSDGSD
                     RLTFRDPETFAVQGQVRVTLEGRPLEQINELECVDGQVWANVWLRDQIVRIDPATGRV
                     TAVVDASGLL"
     gene            complement(789..3335)
                     /locus_tag="VEL74_12530"
     CDS             complement(789..3335)
                     /locus_tag="VEL74_12530"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HYG63402.1"
                     /translation="MKSSLFAFCLVAPSLVPGAAAAPAAAKPAAAPAADSGLPTVAKA
                     TAGLDRREGLLNLHYDRQKGKVWLELPPASGPRGEVGTFIYVESLRTGLGSNPVGLDR
                     GQLGSTRLVTLRRMGGRLLLEQPNLKFRARSENADELQAVRESFATSVLWAGDVTAQD
                     PDGRALVDFTSFLVRDAHDLPRSMRLAGQGSWSLDPARSAVDFENCLSFPKNLELEAL
                     LTYTSSDPGALVRETAPTGSVVSVTQHHSLIALPEPGYQTREFDPRAGSYSLDFMDYA
                     APLDAPVETKLVARFRLEKTDPTAARSTVKKPIIFYVDPGAPEPVRTALVEGAGWWRE
                     AFDKAGFVDAYRVELLPPGAHPLDVRYNVIQWVHRSTRGWSYGGGMIDPRTGEILKGH
                     VTLGSLRVRQDRLLFEGLTDAERTGKGGPDDPIQLSLWRIRQLAAHEVGHALGLNHNF
                     AASTYGGRASVMDYPAPLVKLTTDGQMDFSETYGTGMGEWDVHSIRFAYSQFPPGTDE
                     AFALDAIAREGVGRGYLFLTDEDARGLGSAHPRAHLWDNGPDPAEGLRHAMKVRGLAL
                     GRFGEKNLAPGRPLSLIQEVLAPVYFHHRYQLEAAVKVVGGLEYTYAVRGDGQPASRP
                     VDAEWQRRALSAVLATLSPEALDLPDPLIALLLPRPSGYLPNRELFKGWTDPTFDPLG
                     AAGAAADMAVLGLLQPERAARLVDFHRRDPKMPGLDEVLDGLVGAAFGTGAGKETERQ
                     AELRRTVQWVVVRRLIDLSANANASPGVRARVDAQLAALARRPGPNDALAHREFLARE
                     IGRYLDRRAQEATARPAPPEAPPGQPIGMPDPAWAPESLPEPLAGCSWEP"
     gene            3466..5502
                     /locus_tag="VEL74_12535"
     CDS             3466..5502
                     /locus_tag="VEL74_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYG63403.1"
                     /translation="MISGKLVVVTGHLFQAVCAAMIAVVLLVFYRHYRRRYLLHWAWS
                     WWAFCLYVGGAAMAQSSPSPAWVLPATTLSVIGGYAQIALLMVGTWELARGHDLARGK
                     TRRAVGLLVVFGLLMVLVTVASPYPVRLLVRIGLRTFLAGLAFLAASVGVLRHSGSGM
                     GPKVLGGAFLVYALEQFHYTTIMGLPLLDVDVDLPYLPFLGLFDFLLQTVMGMGMVLW
                     LLEEERRRVLSAAGRIEHLAYHDTLTGLPNRSLLLENLRTAMARSEVDGRKAAVLLLD
                     LDRFKLVNDSLGHAAGDRLLQMVAERIQQVARPGDTVARPGADEFVVLLPGLANEGEA
                     LRMAERLLGVIRQPLSLDGREIVVTASLGISRFPKDGSNPEDLLHKADLAMSQAKGRG
                     RDTFQIHTVSMDTRALERLSLEADLRRALDGDELLLFFQPILDVRTRRIEGGEALLRW
                     RHPERGLLRPNDFLWVAEPSSLSHQLDFWVLDCACRELRRWQAAGSRLRAAVNLSARS
                     LHHPELIRRVEDLLEENGLLPSSLELEIPERLAMQNAEVTLDVVRSLKDLGVGVALDD
                     FGTGYALPYLRSLPVNTLKIDISFVRALGEGPGSAELPAAMMTLARNLGIRVVAEGVE
                     HEKQWELLRHQGCDQVQGHLFSPPLPAEEFRELLLRRRLPEWEGLAARAEPAGL"
     gene            5587..6846
                     /locus_tag="VEL74_12540"
     CDS             5587..6846
                     /locus_tag="VEL74_12540"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYG63404.1"
                     /translation="MPSKIDMVYTENMQTAAIPQHPVETTDERFESLGLSPPILRMIG
                     NLGFEHPTPIQAKVIPVALTGRDLIGLAETGSGKTAAFCIPLAERLARGESARALILC
                     PTREIALQTQAFLDLFGQEYGLKTACLIGGVKMGPQVRAVQARPAVVVATPGRLLDHV
                     ERRTLRLDGINELVLDEADHMLDMGFMPQVNRILQLLPEKRRTMMFSATMPEPIEKLA
                     QRFMADPLRVDILPEGGTASGISHRLYMVDPENKKACILALLHLELGSTLVFTRRRTD
                     AEWLCGVLEREGHPVERIHSDLNQGQRVEALRGFREGEHRILVATDIAARGIDIPQIQ
                     HIINFDMPETVEDYIHRAGRTARGTAHGIVSSIASWMDKATIKEIEQTLGEDLPRCTV
                     PGVEPWVEMKPKNVLGGNRRGLKVKRR"
     gene            complement(6967..8229)
                     /locus_tag="VEL74_12545"
     CDS             complement(6967..8229)
                     /locus_tag="VEL74_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin D"
                     /protein_id="HYG63405.1"
                     /translation="MKRRFTKTIALAVAACLCAGPALPFTQGAGIRGTGGTPQGHEWL
                     TVRSALEVLGDESGDSPSDPRTKNQLPRAENPNITPEMAAAFKRQTTGEKTYGARYQK
                     VLDAVLGQRWVDIGGFNVVKAQSAKYNCFDMVPQLPDDIQRDHFMRMRGDAGGAGAVA
                     AMNDGVARFVQYFVDAATAPEGQLKAWDGGGYSVKIEADRNYFLFGRAIHLFQDSFSA
                     DHTVRLPADGYRSLQGIKTYLCSLHSDQHSRNKPFAIDFNLDYAKVGDIIWLNRRNDW
                     TPANVKLNALVAVEGMKDAWASFIRVMALPQAQRQGAARAEAERIARAWLSFDPEAVR
                     QRYDDPDAAKKIEGFVSDQAACDNTIGGPGLMERIRNERAICLWNVQAVDGTTERDRH
                     LHIPFRWDWKSSVSWKTPPPDWDPTKEN"
     gene            8381..8581
                     /locus_tag="VEL74_12550"
     CDS             8381..8581
                     /locus_tag="VEL74_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63406.1"
                     /translation="MLPGLDGREAVGRLRVFDEDLVSAVNVAIHLLQAPESLAWLLEA
                     AGGVALERCGVILDERVAEAGG"
     gene            8941..10755
                     /locus_tag="VEL74_12555"
     CDS             8941..10755
                     /locus_tag="VEL74_12555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYG63407.1"
                     /translation="MRFRGLSRYAHFLIAGALLAVLAVLAVHQYHWIHNVSELHRERM
                     RRDLRSTGIRFTRDFNREVTRAFLYFHPDFAERTPWRELAARQLERWRTEAPYPRLVR
                     DVFAVRRTDSGELILEALVSDAPEGGRFRDIPWPADLEPLRRRLSDGHSHFDMTSPVS
                     ADVPGLVVPLPFQPFEPAGGGAFIREGKHRWNEYLVVRLDTGVIAGEILPALTTEHFG
                     SPRGVDYAVAVVATDNPARVIYRSDPSLPAAAFRTGDMQLGLLAVGPFEELRRLLPRT
                     RGERGEGHPGHIRRHFRIQVDPPSAPDAPDVEGVEGREPRRAAGWQLVIRHRNGTLED
                     AVARVRRTQMAAGAGMLALLGVTSVLLVIATQRTQALARQQIEFVAGVSHELRTPLTA
                     IRSAGQNLADGVVTDPSQVKRYGVLIASEGRRLSNMVGQVMEFAGFQSNHKNYELQPT
                     AVADVLEGALEESRWLLEERHAQVEKDIAAGLPPVLADAPALRRAVGNLLENAAKYGG
                     RPPRIGVRARTEGREVEVTVEDHGPGIGKEDLPRLFEPFYRGRNGSTRGIPGSGLGLS
                     IVRHIAEAHRGRVSVTTNPGQGSAFTLHLPAAPGEEAA"
     gene            10752..11465
                     /locus_tag="VEL74_12560"
     CDS             10752..11465
                     /locus_tag="VEL74_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYG63408.1"
                     /translation="MIAARSQKPSRRILLVEDEPSLVLTLTDRLIAEGYEVETAGDGE
                     TGVELALQGSFDLVLLDVMLPGKDGFEVCRELRQHGIEVPILMLTARGQVVDRVVGLK
                     LGADDYVVKPFEMIELLARMEALFRRSRPAGGPASAGTYAFGDVRVDFRRGEVFRDGE
                     PVALSALEFKLLTYFIQNRGTLLSRDELLDKVWGYDAMPTTRTVDVHVASLRQKLERN
                     PSHPEFILTVHRRGYRFAG"
     gene            11593..12072
                     /locus_tag="VEL74_12565"
     CDS             11593..12072
                     /locus_tag="VEL74_12565"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HYG63409.1"
                     /translation="MKKIAFAMMVALLLTVAAFHAVQAGEHKIVHRTVMGGPGFGEHV
                     ASALDLTEDQKAEAKKIHEEVFAKSQPLMEQHSQQMEAVHALLDGDNPDATEVGTKMI
                     AAHSYMKQVRTLHDEAMARFSTLLTAEQKAKFDELRKSHDGEFGHRVRFMHRRPGRD"
     gene            complement(12148..>12367)
                     /locus_tag="VEL74_12570"
     CDS             complement(12148..>12367)
                     /locus_tag="VEL74_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HYG63410.1"
                     /translation="AVPDPFAARRMLEVARMVNPGVETIVRTHSEEESELLVKERAGK
                     VFMGEHELALSMTRSILERVAEARPAAH"
BASE COUNT         1804 a         4338 c         4361 g         1864 t
ORIGIN      
        1 gaggaggccc gaggcgtcca ccacggcggt gacccggccg gtggcagggt cgatgcgcac
       61 gatctggtcg cggagccaga cgttggccca gacctggcca tcgacgcact ccagctcgtt
      121 gatctgctcc agcggccggc cttcgagggt cacccgcacc tgcccctgga cggcgaaggt
      181 ctcgggatcg cggaaggtca gccggtccga gccgtcgctc atcaccaggc gcttgccgtc
      241 gtggcagagc ccccagccct cgccggtata ggtgagctct ttcagcttgc cgaagtccga
      301 cagccggtag accagggcgg tcccctcgtg ccaggtgagc tggatcagcc ggtcccccac
      361 cagggtcagc ccctcggcga agtattgcgg cggcaggtcc agccggcgcc gtacctcgcc
      421 cgtggccggg tccacctgcc gcaggctcga ctgcccgtaa tggccggcgc tctcgtagag
      481 gctgccgtcg tgccagagca ggccctgggt gtaggactgg ggatcgtgag ggcggatgga
      541 gacgacctgc gcccgcagcc gctccggacc cggagccgcc gcctgggtgg cgggcggcgc
      601 ggcgggacgg gacggctcgg cggggggctg gcccgccccc cgcagccacc agaacgagag
      661 cagggcgacc gcgagaccgc tcatgaggac aatgatgccc catttcgcgg ctgatttcgc
      721 gggcgctttc ccgggcggcg ccgggggcgg cgcgccggcc cccggcttcc cggtgtcacg
      781 cttccggctc acggctccca cgagcacccg gccaggggct cgggcagcga ctcgggcgcc
      841 caggccggat cgggcatgcc gatcggctgt ccgggcggcg cctcgggcgg cgcgggacgg
      901 gccgtcgcct cctgcgcccg gcggtcgagg tagcgcccga tctcccgcgc caggaactcg
      961 cggtgcgcca aggcgtcgtt cggccccggc cggcgcgcca gggcggcgag ctgggcatcc
     1021 acccgcgccc gcacgccggg cgaggcgttg gcgttggccg acaggtcgat cagccggcgt
     1081 accacgaccc actgcacggt gcggcgcagc tcggcctggc gctcggtctc cttgcccgct
     1141 ccggtgccga aggccgcacc caccagcccg tccagcacct cgtcgagccc cggcatcttc
     1201 gggtcgcggc gatggaagtc caccagccgg gcggcgcgct cgggctggag cagccccagc
     1261 accgccatgt ccgccgccgc ccccgccgcg ccgagcgggt cgaaggtggg atcggtccac
     1321 cccttgaaca gctcgcggtt cggcaggtac cccgacggcc gggggagcag cagcgcgatc
     1381 agcgggtcgg gcaggtccag cgcctcgggc gacagggtgg ccagcaccgc cgacagcgcc
     1441 cggcgctgcc actcggcgtc caccggcctg gaggccggct ggccgtcgcc gcgcacggcg
     1501 tacgtgtatt cgaggccgcc caccaccttg accgccgcct cgagctggta gcggtgatgg
     1561 aagtagaccg gcgccagcac ctcctggatc agggacaggg ggcggcccgg cgccaggttc
     1621 ttctcgccga agcggccgag ggcgaggccg cgcaccttca tggcgtggcg cagcccctcc
     1681 gccgggtccg gcccgttgtc ccacaggtgc gcccgcgggt gggcggagcc cagcccccgg
     1741 gcgtcctcgt cggtgaggaa gaggtagccg cggccgaccc cctcgcgggc gatggcgtcc
     1801 agggcgaagg cctcgtccgt gccgggaggg aactggctgt aggcgaagcg gatggagtgg
     1861 acgtcccact cccccatccc ggtgccgtag gtctccgaga agtccatctg cccgtcggtg
     1921 gtcagcttca ccagcggcgc cgggtagtcc atcaccgagg cccgtccgcc gtaggtcgag
     1981 gcggcgaagt tgtggttgag gccgagggcg tgccccacct cgtgcgccgc gagctgtcgg
     2041 atgcgccaga gcgagagctg gatcgggtcg tccggccctc ccttgccggt acgctcggcg
     2101 tcggtcagcc cctcgaagag gagacggtcc tggcgcacgc ggagcgatcc cagggtgacg
     2161 tgccccttga ggatctcgcc ggtccgcggg tcgatcatgc cgccgccgta ggaccagccg
     2221 cgggtcgacc ggtgcaccca ctggatgacg ttgtagcgca cgtccagggg gtgggcgccg
     2281 ggcggcagca actccacccg gtaggcgtcg acgaagccgg ccttgtcgaa ggcctcgcgc
     2341 caccagcccg caccctccac cagggcggtg cgcaccggct ccggcgcgcc cgggtcgaca
     2401 tagaagatga tgggcttctt gaccgtcgag cgcgccgccg tcggatcggt cttctccaga
     2461 cggaagcggg cgacgagctt ggtctcgacc ggcgcgtcga gcggcgcggc gtagtccatg
     2521 aagtcgagcg agtaggagcc ggcgcggggg tcgaactccc gcgtctgata gcccggctcg
     2581 ggcagagcga tcagcgagtg gtgctgcgtg acgctgacca cggagccggt gggcgcggtc
     2641 tcccgcacca gcgcgcccgg atcgctcgac gtataggtca gcagcgcctc gagctccagg
     2701 ttcttcggga agctcaggca gttctcgaag tcgacggcgc tgcgggcggg gtcgagcgac
     2761 cagctcccct ggccggcgag ccgcatgctg cgcggcaggt cgtgggcgtc gcgcaccagg
     2821 aacgaggtga agtcgaccag cgcccggccg tccgggtcct gggccgtgac gtcgccggcc
     2881 cacagcaccg aggtggcgaa cgactcgcgc accgcctgga gctcgtcggc gttctcggag
     2941 cgggcgcgga atttcaggtt cggctgctcg agcaggagcc tcccccccat ccgccgcagc
     3001 gtcaccagac gggtggagcc gagctggccg cggtcgagcc ccaccgggtt ggagccgagg
     3061 ccggtgcgca ggctctccac atagatgaac gtccccacct cgccgcgcgg gcccgaggcc
     3121 ggagggagct ccagccacac cttgcccttc tggcggtcat agtggaggtt gagcagcccc
     3181 tcgcggcggt cgagcccggc ggtggccttg gctaccgtgg ggagcccgct gtcggcggcc
     3241 ggagccgcgg ccggcttggc ggccgccggg gccgccgcgg cccccgggac gagggatggc
     3301 gccacgagac agaaggcaaa cagagaagac ttcaaagctg aaggcatcca attcatatca
     3361 aggcctcatg ggaagattgg gaaaagcccg cagagtctaa caccggcgcg ctgtgacaga
     3421 gatatgacgg aggctggccg gatctgataa cctttcgcct gtctgatgat cagcggaaaa
     3481 ctcgtggtgg tcaccggcca cctctttcag gcggtctgcg ccgccatgat cgcggtggta
     3541 ctactggtgt tttaccggca ctatcgccgg cgatacctgc tgcattgggc ctggagctgg
     3601 tgggccttct gcctctacgt cggcggcgcg gccatggctc agagctcgcc gtcgccggcg
     3661 tgggtgctgc cggcgacgac cctctcggtg atcggcggct atgcccagat cgccttgctc
     3721 atggtcggca cctgggagct ggcgcggggg cacgacctcg cgcgcgggaa gacgcgccgg
     3781 gcggtggggc tgctggtggt cttcggcctg ctcatggtcc tggtcacggt cgcctccccc
     3841 tatccggtcc gcctgctggt gcgcatcggc ctgcggacgt tcctcgccgg cctggcgttc
     3901 ctggcggcgt cggtgggagt gctaaggcac tccggcagcg gcatggggcc caaggtcctc
     3961 ggcggggcct ttctggtgta cgccctggag cagttccact acaccaccat catgggcctg
     4021 ccgctcctgg acgtggacgt cgacctgccg tacctgccgt tcctcggcct gttcgacttc
     4081 ctgctgcaga ccgtcatggg gatggggatg gtgctctggc tgctggagga ggagcgccgg
     4141 cgggtcctct ccgccgccgg gcgcatcgag cacctggcct atcacgacac gctgaccggg
     4201 ctgcccaacc gcagcctgct gctggagaac ctgcggaccg ccatggcccg ctccgaggtg
     4261 gacggccgca aggccgccgt cctcctcctc gacctcgacc gcttcaagct ggtcaacgac
     4321 tcgctcggcc acgccgcggg cgaccggctg ctccagatgg tggccgagcg catccagcag
     4381 gtggcgcggc ccggcgacac ggtggcgcga cccggcgccg acgagttcgt ggtgctgctc
     4441 cccggcctcg ccaacgaggg ggaggcgctc cgcatggccg agcggctcct cggcgtcatc
     4501 cgccagcccc tgtcgctgga cggccgggag atcgtggtca ccgccagcct cggcatcagc
     4561 cgcttcccca aggacggctc caaccccgag gacctgctcc acaaggccga cctcgccatg
     4621 tcccaggcca agggccgggg gcgcgacacc ttccagatcc acaccgtcag catggatacc
     4681 cgggcgctcg agcggctttc gctcgaggcc gacctgcgcc gcgccctgga cggcgacgag
     4741 ctcctcctct tcttccagcc gatcctcgac gtccgcaccc gccgcatcga agggggagag
     4801 gcgctgctgc gctggcgcca ccccgagcgc ggcctgctgc ggccgaacga cttcctgtgg
     4861 gtggcggagc cctccagcct ctcgcatcag ctcgacttct gggtcctgga ctgcgcctgc
     4921 cgggagctgc ggcgctggca ggcggcgggg tcgcggctgc gggcggcggt caacctctcc
     4981 gcccgctcgc tccatcaccc ggagctgatc cggcgggtcg aggatctgct ggaggagaac
     5041 ggcctgctcc ccagctcgct ggagctggag atccccgagc ggctggcgat gcagaacgcc
     5101 gaggtgaccc tggacgtggt ccgctccctc aaggacctgg gcgtcggcgt cgccctggac
     5161 gacttcggca ccggctacgc cctcccctac ctgcgcagcc tgccggtcaa cacgctgaag
     5221 atcgacatct cgttcgtccg cgccctgggc gaggggccgg ggagcgccga gctgccggcg
     5281 gcgatgatga ccctggcgcg caacctgggg atccgggtgg tagccgaggg ggtggagcac
     5341 gagaagcagt gggagctgct gcgccaccag ggttgcgacc aggtgcaggg gcacctgttc
     5401 agcccgccgc tgccggcgga ggagttccgc gagctgctgc tgcggcggcg cctgccggag
     5461 tgggaagggc tggcggcgcg cgccgagccc gccggtctgt aagcgccttt tgaccggcac
     5521 gccgggaaat catttgtcac ccgcggccgt tctgtggaat ggacgcgtgc gcgggagctc
     5581 cttcccttgc cttcgaagat cgacatggtc tatacagaaa acatgcagac tgctgccatt
     5641 ccacaacacc ccgtagaaac caccgacgag aggttcgaat ccctcggcct ctccccaccc
     5701 atcctccgga tgatcgggaa cctcggcttc gagcacccga ccccgatcca ggccaaggtc
     5761 atccccgtcg ccttgaccgg ccgggacctc atcggcttgg cggagaccgg ctcgggcaag
     5821 accgccgcct tctgcatccc gctcgccgag cgcctggccc gcggcgagag cgcccgcgcc
     5881 ctgatcctct gccccacccg cgagatcgcc ctccagaccc aggccttcct cgacctcttc
     5941 ggtcaggagt acggcctcaa gaccgcctgc ctgatcggcg gcgtcaagat gggtccccag
     6001 gtgcgggcgg tccaggccag gcccgccgtg gtggtggcca cccccggccg cctgctcgat
     6061 cacgtggagc gccgcaccct gcggctcgac gggatcaacg agctggtgct ggacgaggcc
     6121 gaccacatgc tggatatggg cttcatgccg caggtgaacc ggatcctgca gctcctgccc
     6181 gagaaacgcc gcaccatgat gttctcggcg acgatgcccg agccgatcga gaagctggcg
     6241 cagcggttca tggcggaccc gctgcgggtc gacatcctcc ccgagggggg caccgcttcc
     6301 ggcatcagcc accgcctcta catggtggac ccggagaaca agaaggcctg catcctggcg
     6361 ctgctccatc tggagctggg gagcacgctg gtgttcaccc gccgccgcac cgacgccgag
     6421 tggctgtgcg gcgtcctgga gcgggagggg catccggtgg agcgcatcca ctcggacctc
     6481 aaccagggcc agagggtgga ggcgctgcgc ggcttccgcg agggcgagca ccgcatcctg
     6541 gtggcgacgg acatcgcggc gcggggcatc gacatcccgc agatccagca catcatcaac
     6601 ttcgacatgc ccgagacggt ggaggactac atccaccgcg ccggccgcac cgcccgcggc
     6661 acggcccacg gcatcgtctc ctccatcgcc tcctggatgg acaaggcgac gatcaaggag
     6721 atcgagcaga cgctgggaga ggatctcccc cgctgcacgg tccccggcgt cgagccctgg
     6781 gtcgagatga agcccaagaa cgtcctcggc ggcaaccgcc gggggctcaa ggtcaagcgg
     6841 cgctgagcgc tgttcgaagg cgctcgaagc gccgaggcgg caggggtcgg ctcgcgccct
     6901 cccctgccgg cctgacagga aaccctccca cccttttcct tctccagcct cctgcccggt
     6961 cgagcctcag ttctccttcg tcgggtccca atccggaggc ggcgtcttcc aggagacgct
     7021 gctcttccag tcccaccgga aggggatgtg gaggtggcgg tcccgctcgg tggtgccgtc
     7081 gaccgcctgg acgttccaca ggcagatcgc ccgctcgttc cggatcctct ccatcagccc
     7141 ggggccgccg atggtgttgt cgcaagcggc ctggtcgctg acgaagccct cgatcttctt
     7201 cgccgcgtcg gggtcgtcgt agcgctgccg caccgcctcg gggtcgaagg agagccaagc
     7261 gcgggcgatg cgctcggcct cggcgcgggc ggcgccctgc cgctgagcct ggggcagcgc
     7321 catgacccgg atgaacgacg cccaggcgtc cttcatcccc tcgaccgcca ccagggcgtt
     7381 gagcttgacg ttggccggcg tccagtcgtt gcggcggttc agccagatga tgtctcccac
     7441 cttggcgtag tcgagattga agtcgatggc gaacggcttg ttgcgcgagt gctggtcgct
     7501 gtgcaggctg cagaggtagg tcttgatccc ctgcaggctt cggtagccgt cggcgggcag
     7561 gcgcaccgtg tgatcggcgc tgaaggagtc ctggaagagg tggatggcgc ggccgaagag
     7621 gaagtagttg cggtcggcct cgatcttcac cgagtagccg ccgccgtccc aggccttgag
     7681 ttggccttcg ggcgccgtgg ccgcgtcgac gaagtactgg acgaagcggg cgacgccgtc
     7741 gttcatggcc gccacggcgc cggcgccgcc cgcgtcgcca cgcatgcgca tgaagtggtc
     7801 gcgctggatg tcgtccggga gctgcggcac catgtcgaag cagttgtact tggccgattg
     7861 agccttgacg acgttgaagc cgccgatgtc gacccagcgc tggccgagga cggcgtcgag
     7921 gaccttctgg tacctggcgc cgtaagtctt ctcgccggtg gtctggcgct tgaaagcggc
     7981 ggccatctcc ggggtgatgt tggggttctc ggcacgggga agctggttct tggtgcgcgg
     8041 gtcgctcggc gagtccccac tctcgtcgcc cagcacctcg agggcggagc ggacggtgag
     8101 ccactcgtgc ccctgcggcg tgcccccggt gccgcggatg cccgccccct gggtgaacgg
     8161 cagggccggg ccggcgcaca ggcaggccgc gaccgccagg gcgatggtct tggtgaatct
     8221 cctcttcatg atgctctcct cgtgatcgtc gcggccccgg ggatggagcc gcggaattac
     8281 aggagagctg tcacgagggg gcgggttatc tcagggacga gagggggacc cgcgggtccg
     8341 tatcgagaag caggccgatg aggacggcgg ctatcccctc gtgcttccag gcctggacgg
     8401 ccgggaggcg gtgggacggc tccgggtgtt cgacgaggat ctcgtcagcg ccgtcaacgt
     8461 ggcgatccac ctgctccagg cgccggagtc cctcgcctgg ctgctggagg cggcgggcgg
     8521 cgtcgccttg gagcggtgcg gggtgatcct cgacgaacgg gtggcggagg cgggcgggta
     8581 ggtaggttgc cgtaccaggg ccaccggcgg gcctgggcct gccggtggcg cgccacatcc
     8641 tgccgcccgg tcaccaaggg atcgaagagt ctggcggact ccccagagtc atggcggagg
     8701 gaacccagaa gtcgggatga ccccgagaaa gtcacgaagg atccagaggt cgggatgaac
     8761 cccccggaag tcatggagga aatccacaag tctggatgac tcccggagag tcagcgagga
     8821 atccaaaagt atggatcgac ccgcggaagg tcagcgagtc ctcaagccgc cgcaagggat
     8881 ttgtaaggga tgtaaaggct tcaaccggca gggcttcgcg gctgctagac tggcgaggtc
     8941 atgcggtttc gcggcctctc ccgctacgct cacttcctca tcgccggggc tctcctggcg
     9001 gtgctggccg tgctggcggt gcatcaatat cactggatcc acaacgtcag cgagctgcat
     9061 cgcgagcgga tgcggcggga cctgcgctcc accggcatcc gcttcacccg cgacttcaac
     9121 cgcgaggtga cgcgggcgtt cctctacttc catccggatt ttgcggagcg cactccgtgg
     9181 cgcgagctgg cggcgcggca gctcgagcgc tggcgtaccg aggcgcccta cccccgcctc
     9241 gtgcgcgacg tcttcgcggt gcggcggacc gattcggggg agctgatcct cgaggcgctc
     9301 gtctccgacg cgccggaggg cggccggttc cgggacatcc cctggccggc ggacctggag
     9361 ccgttgcgcc ggcgtctctc cgatggccac tcccacttcg acatgacctc gcccgtctcg
     9421 gcggacgtcc cggggctggt ggtgccgctc cccttccaac ccttcgaacc ggctggcggc
     9481 ggcgccttca tccgggaagg aaagcaccgc tggaacgaat atctggtcgt gcggctggac
     9541 acgggggtca tcgccggcga gatcctcccc gctctcacca cggagcactt cgggagccct
     9601 cgcggggtgg actatgccgt ggctgtcgtc gccacggaca atcccgcgcg agtgatctat
     9661 cgctccgatc cgagcctccc cgccgcggcc ttccgtaccg gtgacatgca gctcggcctc
     9721 ctcgccgtgg ggcctttcga ggagttgcgc aggctcctgc cccgaacgag aggagagaga
     9781 ggagaggggc accccgggca catccggcgc cacttccgaa tccaggtcga tccgccgtca
     9841 gctcccgacg ctcccgacgt ggaaggtgtg gaggggaggg agccccggcg ggccgccggc
     9901 tggcagctcg tgatccggca ccgcaacggc accctggagg acgccgtggc ccgcgtccgg
     9961 cgcacgcaga tggccgccgg agccggcatg ctggcgctcc tgggggtgac ctccgtgctg
    10021 ctggtgatcg ccacccagcg gacccaggcg ctggcgcggc agcagatcga gttcgtggcc
    10081 ggcgtctcgc acgagctgcg cacgccgctc accgcgatcc gctcggccgg ccagaacctc
    10141 gccgacggcg tggtgaccga tccgtcccag gtgaagcgct acggcgtcct catcgcgagc
    10201 gaggggcggc ggctctcgaa catggtcggc caggtgatgg agtttgccgg cttccagtcg
    10261 aaccacaaga actacgagct gcagcccacg gcggttgcgg acgtcctgga aggcgccctg
    10321 gaggagagcc gctggctgct cgaggagcgg cacgcccagg tcgagaagga catcgccgcc
    10381 ggcctccccc ccgtgctggc cgacgcgccg gccctgcgcc gggcggtggg gaacctgctg
    10441 gagaacgcgg ccaagtacgg cgggcgcccg ccgcggatcg gcgtacgcgc ccggaccgag
    10501 gggcgcgagg tggaggtcac cgtggaggat cacgggccgg ggatcggcaa ggaggacctg
    10561 ccgcgcctgt tcgagccctt ctatcgcggc cggaacggct ccacgcgcgg catcccgggg
    10621 agcggcctgg gcctgagcat cgtgcgccac atcgctgaag cccaccgcgg ccgggtcagc
    10681 gtgaccacca atccgggcca ggggagcgcc ttcaccctgc acctgcccgc ggcgcccgga
    10741 gaggaggcgg cgtgatcgcg gcccgctcgc agaagccgtc tcggcgcatc ctcctggtgg
    10801 aggacgagcc cagcctggtc ctcaccctca ccgaccgcct gatcgccgag ggctacgagg
    10861 tggagaccgc aggggacggc gagaccggcg tcgagctggc gctccagggc tccttcgacc
    10921 tcgtgctcct ggacgtcatg ctccccggca aggacggctt cgaagtctgc cgcgagctgc
    10981 ggcagcacgg catcgaggtc cccatcctga tgctcaccgc ccgcggccag gtggtcgacc
    11041 gggtggtggg cctcaagctg ggcgccgacg actacgtggt caaaccgttc gagatgatcg
    11101 agctgctcgc ccgcatggag gccctgttcc ggcggtcgcg ccccgccggc ggccccgcgt
    11161 ccgcggggac ctacgcgttc ggcgacgtgc gggtggactt ccggcgcggc gaggtcttcc
    11221 gcgacggcga gccggtggcc ctctcggcac tggagttcaa gctcctcacc tacttcatcc
    11281 agaaccgcgg cacgctgctg tcgcgcgacg agctgctgga caaagtctgg ggctacgacg
    11341 ccatgcccac cacgcgcacc gtggacgtcc acgtcgcctc gctgcgccag aagctggagc
    11401 gcaatccctc gcatccggaa ttcatcctga ccgttcaccg gcgcggctat cgtttcgccg
    11461 gttgacgagg cttacatctc ttacaaacaa cttgcggctt cctgaggact cgcctccgga
    11521 ctcggaccta tcctttgaac catgaagagg gcctcgcggg gatgcgggag cctactgatg
    11581 aggagacctg acatgaaaaa gattgctttt gcgatgatgg ttgccctgct cctgaccgtc
    11641 gccgccttcc acgccgtgca ggctggcgag cacaagatcg ttcacaggac cgtgatgggc
    11701 ggccccggct tcggggagca tgtcgcgtcc gccctggacc tgaccgagga ccagaaggcc
    11761 gaggcgaaga agatccacga ggaggtcttc gccaagtcgc agccgctcat ggagcagcac
    11821 agccagcaga tggaagcggt ccacgccctg ctggacggcg acaaccccga cgccaccgag
    11881 gtcggcacca agatgatcgc ggcccactcc tacatgaagc aggtccgcac gctccacgac
    11941 gaggcgatgg cccgcttctc caccctgctg accgccgagc agaaggccaa gttcgacgag
    12001 ctcaggaagt cgcacgacgg cgaattcggc caccgggtcc ggttcatgca ccgccggccg
    12061 ggccgcgact gagcccgaac cgagtccttc cttccagcgg gacggcccct ggacctcctc
    12121 ccggccgccc cgcgcttttt cccgctttca gtgcgccgcg ggccgcgctt cggccacccg
    12181 ttccaggatg gagcgagtca tgctgagagc gagctcgtgc tcccccatga acaccttccc
    12241 cgccctctcc ttcaccagca gctcggactc ctcctcgctg tgagtgcgga cgatcgtctc
    12301 cacgcctgga ttgaccatgc gggccacctc cagcatgcga cgggccgcga aggggtcggg
    12361 aacggcg
//
LOCUS       DASPJI010000461        10249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_25898, whole genome shotgun sequence.
ACCESSION   DASPJI010000461 DASPJI010000000
VERSION     DASPJI010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10249)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10249
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25898"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2131)
                     /locus_tag="VEL74_12575"
     CDS             complement(<1..2131)
                     /locus_tag="VEL74_12575"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HYG63411.1"
                     /translation="MRRTWLVWVLVLVTALPGLGALPVSGEPIPLRGQVVDADGKPLR
                     GASVALLPVVSRFASQELLLQGKALPEPVARTASREDGRFEMSAPSPGMWEMQVTARG
                     FVPRRFSLFPLLEPLEIPRLEMVQEAPLPIRVVDAQGRPVAGAQVRVNAGESPVFTPL
                     SWWWPKPELVTTGTDGTATVSRWKGVEVELEAAAPGHPPAALKVAARESTAEMRLAAA
                     CPRTVQVSTRGRDGAARPVAGALAVHRSWPLGVTGEDGRVTVAAPCERDLGISVETRD
                     GRQARATLRSQRAGRANRTVDPLTRVTLPAEPVRVSGRVLTEGSRAPVAGALVWTGAD
                     PAGFVRTDAQGAYTLTLTAGEPSHVTGDAVGHLADLEEVRAGEGPTFLLTPLAVASGV
                     VVDEAGRPVTGARVRGMADDPAKSGLWNPLLSGSSDAKGEFRLRVLSSEALTLSASHP
                     DFVAADLSIPPVAPHASRSGLRIVLAKGLAASGKVVDAEGRPIAGAEVWLFPSMDGDE
                     GFAFFRQMERRLTPAVTDARGVFTLEHLKPGRMDLQASARGFAPTRVRGLQLTAGDGA
                     VDLGTVTLGPGVTLAGVVVDPADRPVAGATVQLLGTASPLPVTLHRAGEEELLTAADG
                     SFSIPGLAPEERVTLSISRKGFASADFRDLQVPPAEPLRIVLTPGSRIAGRLVDEQGD
                     PVSGMVSLRRIGGQSTGGPIVDHAGTAE"
     gene            2424..2975
                     /locus_tag="VEL74_12580"
     CDS             2424..2975
                     /locus_tag="VEL74_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63412.1"
                     /translation="MRNHRDGSTGERVPSSTPAGPSPNAFTPEYLDLLNQRDEPASAS
                     DADAAGPWHLERTSEGGWAVLRLGESLGRGDVPSGDFIRPEFAKLTAGLLPGTGKSKR
                     FRQGKDHDGRGYPVYYDGDWIGHLPLFDEDLIASLNIADALLSAPNDLAWVLDAAGGV
                     ALEHTGKISLARAREDAGTPAPM"
     gene            complement(3873..5897)
                     /locus_tag="VEL74_12585"
     CDS             complement(3873..5897)
                     /locus_tag="VEL74_12585"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG63413.1"
                     /translation="MTVAGSAQISFGGAAPPLPVSPLHQLPSPPADFTGREAELADLR
                     KAVEQGGATISGLRGMAGVGKTALALKLAEQLKPLFPDAQLYLDLKGVSSTPLFPTQA
                     MSHVVRSYYPDAQLPERQEELAALYRSVLNGKRALLLMDNAAGREQVESLIPPKGCLL
                     LVTSRFHFHLPGLLTRDLDELSPEEAKKLLLKIEPRIGESAAELAGLCGRLPIALRLA
                     GAALTERPDLSPATYVERLKDARKLLDLVEASFTLSYDLLPEDLQKRWRELAVFPGSF
                     DLKAAAAVWAMEPEPADDMLGALLKSSLVDGEDGRYRLHDLARVFAASRLEEAERAEA
                     RRRHAEHYAWVLAAANFLYLKGGDSVLVGLRLYDLEWGNIQAGFAWAAEGAKADEAAA
                     RLCSRYLDAGADCLDLRQPKRERIQWLESALAAARQLGDRSAEGRHLGNLGIAYKNLG
                     DARRATDHYEQVLQIARETGDRRGEGSALGNLGNAYAKLGDARRATEHYEQHLQIARE
                     IGDRRGEGNALGNLGNAYAGLGDVRRAIEHFEQVLQIAQETGDRRGEGQTFGNLGNAY
                     AELGDVRRAIEHHEQRLQIAREIGDRAGEARGSWNLGLLYEAEDDLERAVELMQRQVE
                     YAREIGHQEAEKWAARVDAIRARIQETPSRPKKAPKKKRPTSQPRSGDRI"
     gene            complement(5966..6421)
                     /locus_tag="VEL74_12590"
     CDS             complement(5966..6421)
                     /locus_tag="VEL74_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63414.1"
                     /translation="MVPPPLGGSGGRERGRTGGGFCTAPAKMALHAAGYNSPVLLKGE
                     LMDAQTATLVGLAILALLVIFAVVRYRGKAKFEVKGPGGLGASFEGENQQAPSVQPAA
                     TRPGISMEGVKSRAGGVHAEDRPGQGISMKDVDAHGDVQAISGDSAPKA"
     gene            6532..8214
                     /locus_tag="VEL74_12595"
     CDS             6532..8214
                     /locus_tag="VEL74_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYG63415.1"
                     /translation="MDKPILFAIDDDAEVLKAIERDLRRQYGQRYRVMRASSGPSAVE
                     ALQQLKLRNSPLALLLVDQRMPQMTGVEVLEQALPLFPEAKRVLLTAYADTDAAIRAI
                     NTARVDYYLLKPWDPPQESLYPVLDDLLEDWQASYRPPFEGVRVLGHRWSPRSFEVRD
                     FLARHQVPYQWMDLEADGEARQLLETACGPEAPTARLPVVIFPDGSPLQEPSRGEMAE
                     RLGLRTQAESRFYDLVIVGGGPSGLAAAVYGASEGLRTLMIEGDAPGGQAATSSRIEN
                     YLGFPAGLSGADLARRAVAQATRFGVEILTPQEAAGVRLEDPYRIVVLGDRSEVSCHA
                     LLISTGVSYRKLEVPGADKLTGAGIYYGAAMTEAMSCRDEDVYIVGGANSAGQAAMHF
                     ARYASKVVLLVRGGSLAKSMSQYLIDQISETPNIEVRTRTHVAEAHGEDHLEELVLAR
                     EDGDESARETVPARSLFVFIGAQPRTGWLEGVVERDEHGFILSGPDLPKGSDGRRPRG
                     WPLDRDPFLLEASVPGIFVAGDVRHGSVKRVASGVGEGAIAVSFIHQYRSRL"
     gene            8230..9654
                     /locus_tag="VEL74_12600"
     CDS             8230..9654
                     /locus_tag="VEL74_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG63416.1"
                     /translation="MLHDPREQKMFPRLEGEVLQELVELGTELELQDGDRLFSEGDRE
                     YDFFVVLDGQVRITKRVGKEETLLAIHEPGEFTGEISMLTGGPAIANGYAEGPVRVLR
                     LDPNAFRRLMAECAPVARVVLTAMAARATDVDAQLRQQEKLASLGKLAAGLAHELNNP
                     ASAAQRAAGQLRDAACGFLRFSLEREDPLSPEGRRFLCDVHREVLACAGPSSAPPALD
                     PVTQSDREDELTQWLEDRGVQDAWDLAPTLVSAGFDRQSLEEETRGVPDGELEEVVTW
                     ARSILELSAMVDQIETASTRVSDLVSSVKQYSYMDQAPRQEIDLHEGLENTLKILAYK
                     LRGIEVVREYDRTLPRVIAYGSELNQVWTNLIDNAADALDAAGNGKRRVRIRTYRCQE
                     DAVVEVTDDGPGIPEEVQGNIFNAFFTTKPMGQGTGLGLDIARRVVQVRHKGTIRFES
                     RPGETTFEVRLPIGGDSSGAVAAS"
     gene            9711..>10249
                     /locus_tag="VEL74_12605"
     CDS             9711..>10249
                     /locus_tag="VEL74_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017840972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HYG63417.1"
                     /translation="MSERAAVLAWLVASSLLVPATGWAQAEEKAEERERKEKQEAEEP
                     EQAPALQVYEEIEVTGRASDMVGVADSASEGVTGREDLQRRPVMRAGELLETVPGVII
                     TQHSGGGKANQYFLRGFNLDHGTDFRVTVDGIPVNMPSHGHGQGYSDVNFLIPEVVES
                     VRYKKGPYYADEGDFAAAGA"
BASE COUNT         1558 a         3604 c         3573 g         1514 t
ORIGIN      
        1 cctcggcggt gccggcatga tcgacgatgg ggccgcccgt ggactggccg ccgatccggc
       61 ggaggctcac catgccggac accgggtccc cctgttcgtc cacgaggcgg ccggcgatcc
      121 gcgagcccgg ggtgagcacg atgcgcaggg gctcggccgg gggcacctgg aggtcccgga
      181 agtcggcgct ggcgaagccc ttcctggaga tggagagcgt cacccgttcc tcgggcgcca
      241 ggccgggaat ggagaaggag ccgtccgccg cggtcaggag ctcctcttcg ccggcacggt
      301 ggagtgtcac cggcagcggg gacgccgtgc cgaggagctg caccgtggcg ccggcgaccg
      361 gccggtccgc tggatcgacc acgaccccgg caagcgtcac gcccggcccc agggtcaccg
      421 tccccaggtc cacggctccg tcgccagccg tcagttggag gccccgcacc cgggtcggcg
      481 cgaagccgcg ggcggaggcc tggaggtcca tgcgccccgg cttcaggtgc tccagggtga
      541 agacgccgcg ggcgtcggtc acggcggggg tgagccgccg ctccatctgc cggaagaagg
      601 caaagccctc gtctccgtcc atcgagggga agagccacac ctcggcgccg gcgatgggcc
      661 ggccctcggc gtccaccacc ttgccggagg cggccaaccc cttggcgagg acgatccgca
      721 gcccggaccg ggaggcgtgc ggcgccaccg gcgggatgga gaggtccgcg gcgacgaagt
      781 ccggatggct ggcgctcaac gtgagggcct cgctggacag cacgcggagc cggaactccc
      841 ccttggcgtc gctgctgccg gacagcaggg gattccagag gccgctcttg gcgggatcgt
      901 ccgccattcc ccggaccctg gcgccggtga ccggccggcc cgcctcgtcc accacgacgc
      961 cgctcgcgac ggccaggggc gtcaagagga aggtggggcc ctctccggcc cgcacctctt
     1021 cgaggtcggc caggtgcccc accgcgtccc cggtgacatg gctcggctct ccggccgtga
     1081 gggtcagggt gtaggccccc tgggcgtcgg tgcggacgaa gccggcggga tcggcgcccg
     1141 tccagaccag ggcgccggcc accggagccc tggagccctc agtgagcacc cggccggaga
     1201 cgcggaccgg ctctgccggc agcgtcaccc gggtgagagg gtccaccgtc cggttcgccc
     1261 ggcccgcccg ctgggagcgc agggtggccc gggcctgccg gccgtcccgg gtctcgacgg
     1321 agatccccag gtcccgctcg cacggcgcgg cgaccgtcac ccggccgtcc tcgccggtga
     1381 cgcccagcgg ccaggagcgg tgaacggcca gggcgcccgc caccggccgc gccgcgccat
     1441 cccttccccg cgtgctcacc tggaccgtgc gcgggcaggc cgccgccaga cgcatctcgg
     1501 cggtgctctc cctcgccgcc accttcagcg cggccggcgg atggccggga gcggcggcct
     1561 ccagctcgac ctccaccccc ttccagcggg agaccgtagc cgtgccgtcc gtccccgtag
     1621 tcacgagctc cggcttgggc caccaccacg acagcggggt gaagaccggc gactccccgg
     1681 cgttgaccct cacctgcgcc cctgccacgg ggcgcccctg ggcatcgacg acgcgaatag
     1741 ggagcggcgc ctcctggacc atctccagcc gggggatctc caggggctcc aggaggggga
     1801 acagggagaa gcggcgaggc acgaaaccgc gggcggtcac ctgcatctcc cacatccccg
     1861 gggaaggagc ggacatctcg aaccggccgt cctcgcggct ggcagtccgg gccaccggtt
     1921 ccggcaaggc cttgccctgc agcagaagct cctggctggc gaagcggctc accaccggca
     1981 gcagcgccac gctcgctccg cgcagaggct tcccgtcggc gtccaccact tggccccgca
     2041 gcgggatcgg ctctccggag accggcaacg ctccgagccc cggcagcgcc gttaccaaca
     2101 ccagcaccca taccagccag gtccgtctca tcgcccttcc tttcagaagt cgagcatgtc
     2161 cgcttccaaa aggatatacg gatggaagcg gcgctgtgtt caggaagagg tggggaggta
     2221 gggggttccg tttctccggc ccccctcgac ggaatctttt cgagaccgcc tctttcctat
     2281 tgatttcctc ccgcgtcctc gattacgctt gaaggcgcag taggcaggaa agcggccggc
     2341 gtgtagccgg ccacaaaaag gagcgggcac gggtgaggat gagagctcac ccgagcccta
     2401 gtcacgcaag gagtctaagt ccaatgcgca accatcgcga tggtagcacc ggagaacgtg
     2461 taccctcttc cacccccgcc gggccttcac cgaacgcctt caccccggag tatctggacc
     2521 ttctgaacca gcgcgacgag ccggcgtccg cgagcgacgc ggacgcggcc ggcccgtggc
     2581 atctggagcg gacctccgag gggggctggg cggtgctgcg gctgggggag tcgctcggcc
     2641 ggggtgacgt cccatccggc gacttcatcc gtcccgagtt cgccaagctg accgccggcc
     2701 tcctccccgg caccgggaag agcaagcgct tccgtcaggg gaaggaccac gacggccggg
     2761 gctaccccgt ctactacgat ggcgactgga tcggccatct ccccctcttc gacgaggacc
     2821 tgatcgcctc gctgaacatc gccgacgccc tgctcagcgc cccgaacgac ctcgcctggg
     2881 tcctcgacgc cgccggaggc gtggcgctgg agcacaccgg caagatctcc ctcgcccgcg
     2941 cccgggagga tgccggcacg cctgcgccta tgtaataagg aagcggacgc ttcccggggc
     3001 ggccccgccg ccccgggccc tcaccccccg gccccctctc ccgcctcccc tggccccctc
     3061 cccgggagag ggggaggctt gtttgggctt catccggacg gcctggactt tccggcctcg
     3121 ctggagcgcg ggaggtggtg cctccccccc tgggcgggcg ggagggaggg agcgggggag
     3181 gacaggaggg gggttcttct ttttcagccg cgaagcggcg caggcataga gccccaacat
     3241 gcaacgtcac ccgctcccgc cgcctctccg gaaggattct tcatcgcaga gcagcgttcc
     3301 cgcgggtcgt tacccggcta gaacgtcgca gagccgcact ccttcgtcgc cgatccgcac
     3361 agctctgcga cgtccgtgtg cagctctgcg acgttcgaac accaatctgc gacgattgag
     3421 gggaactctg cgacgaatga gcattcccct gcgatgattc agaggtactc tgcgacgttc
     3481 cagggcagct ctgcgacgat cttctcgccg ccgtagtcgt gcgtgccggg acagacccca
     3541 tcgcgtggga gagatcagga ggcggagccg tagcggagcg agaaggtgcc tccccttgcc
     3601 ttggcgctac cttgtaatcc caagggggag gcggtgcctc cccccctggg agggcgggag
     3661 gtcgggagcg ggggaggcca ggaggggggt tcgtcttttt cagccgcgaa gcggcgacag
     3721 aataaagccc cgggcgtgag cccggggaag acgcccccca catcccccag ccccagcggg
     3781 gcgacacgaa caacgttccg ggagaggtgg agtgccgccc ctgccggggc tgtgttttca
     3841 atggattggc ttcctggcgc tcacgcgcca ggctaaattc tgtcgccgct ccgcggctgg
     3901 gaggtggggc gcttcttctt gggtgccttt ttcgggcggc tcggggtttc ttggatccgg
     3961 gcgcggatcg cgtccacccg ggctgcccac ttctcggcct cctggtggcc gatctcccgc
     4021 gcgtattcaa cctgccgctg catcaactcc accgcgcgct ccagatcgtc ctccgcctca
     4081 tacagcaggc ccaggttcca actgcccctc gcttcccccg cccggtcgcc gatctcgcgg
     4141 gcgatctgga gcctctgctc gtggtgctcg atggcacggc gcacatcgcc aagttcggcg
     4201 taggcgttgc ccaggttgcc gaaggtttgt ccctcgccgc gccggtcgcc ggtctcttgt
     4261 gcaatctgga gaacctgctc gaagtgctcg atggcgcgac gcacatcgcc aagacccgcg
     4321 taggcgttgc ccaggttgcc tagggcattg ccttctccgc gccggtcgcc gatctcgcgg
     4381 gcgatttgga gatgctgctc gtagtgctcg gtggcgcgac gcgcatcgcc aagcttggcg
     4441 taggcgttgc ccaggttgcc gagggcgctg ccctcgccgc gccggtcgcc ggtctcgcgg
     4501 gcgatctgga gaacctgctc gtagtggtcg gtggcgcgac gcgcatcgcc aaggttcttg
     4561 taggcaatcc ccaggttgcc gaggtgccgt ccttccgcgg agcggtctcc gagttggcgg
     4621 gcggcggcaa gagcgctctc caaccattga attcgttctc gcttcggctg gcgcagatcc
     4681 agacagtcgg ccccggcatc cagatatcgg ctacaaagcc gcgctgctgc ctcatcggcc
     4741 ttggccccct ccgccgccca ggcgaatccg gcttggatgt tcccccactc caggtcgtag
     4801 agccgcaggc ccaccagaac ggaatcacca cctttcaggt agaggaaatt cgcggcggcc
     4861 aggacccacg catagtgttc cgcgtgcctg cgccgagcct ccgcccgctc cgcctcctcc
     4921 agccggctcg ccgcgaagac ccgggcgaga tcgtgaagcc ggtaccggcc gtcctcccca
     4981 tccacaaggc tgctcttcaa caacgctccg agcatgtcgt cggctggctc gggctccatg
     5041 gcccagaccg ccgccgctgc cttgaggtcg aaggaccccg ggaagaccgc cagttctctc
     5101 cagcgcttct ggagatcctc gggcagcagg tcgtagctca aggtaaagga agcctccacg
     5161 agatcgagca gcttccgggc gtctttcaac ctctcgacgt aggtggcagg ggaaaggtcc
     5221 ggccgctccg tgagggcggc acctgcgaga cggagggcaa tgggtaacct cccgcaaagc
     5281 ccggccaact cggcggcact ctccccgata cggggctcga tctttagcag gagcttcttc
     5341 gcctcctcgg gcgacaactc gtccagatcc ctggtgagga gaccagggag gtggaagtgg
     5401 aaccgcgagg tcacaagcag caggcaaccc ttggggggaa tcagcgattc gacctgctcc
     5461 cgcccggcgg cgttgtccat caggagaagc gcccgtttgc cgttgaggac cgagcgatac
     5521 aaggcggcca gttcctcctg cctctccggg agctgggcgt ccgggtagta ggagcggacc
     5581 acgtggctca tcgcctgagt cggaaagagg ggagtcgagg agaccccctt caaatcgagg
     5641 tagagctggg catccggaaa caagggcttg agctgctcgg cgagcttgag cgcgagggcg
     5701 gtcttgccga ccccggccat cccccgaaga cccgagatgg tggcgccgcc ctgctcgacg
     5761 gctttccgga gatccgccag ctcggcttcg cggccggtga agtcggcggg tggcgagggg
     5821 agttggtgga gaggagagac cggcagagga ggagcggcgc ctccaaaaga gatctgggcg
     5881 ctgccggcca cggtcacgac gtggcctccc gcctggatat gctgctgctc gatgctggcc
     5941 gatccccccg atcctgtgcc gggggtcagg cttttggggc ggagtctccg ctgatggctt
     6001 ggacgtcgcc gtgggcgtcg acatccttca tggagattcc ctggccgggc cggtcctcgg
     6061 cgtggacgcc tccagcgcgg ctcttcactc cttccatgct gatgccgggg cgggtggcgg
     6121 ctggttggac ggagggagct tgctggttct ctccctcgaa ggaggccccg agcccgccgg
     6181 ggcctttgac ctcgaacttg gctttcccgc gatagcgcac gacggcgaag atcacgagca
     6241 gagcgagaat ggctaggccg acgagggtgg cggtttgggc atccatgagc tcacctttca
     6301 ggagaacagg agaattatag ccggctgcgt gaagggccat cttggcgggg gcggtgcaga
     6361 acccccctcc tgtcctcccc cgctccctac ctcccgaccc gcccaggggg ggaggcacca
     6421 cctccctctc aggccgggag tggaggtgag gtacgggact tcgggcgtcg gcttgacgcc
     6481 tcggataaga tcggggttct cctctttgcc ggggggaccg accgaggacc catggacaag
     6541 ccgattctgt ttgccattga tgacgacgcc gaggtgttga aggcgatcga gcgcgacctg
     6601 cgccgccagt atggccagcg ctaccgggtg atgcgcgcca gctcggggcc cagcgccgtc
     6661 gaggcgctgc agcagctcaa gctccgcaac tcccccctgg ccctgctgct cgtcgaccag
     6721 cggatgcccc agatgaccgg cgtcgaggtg ctggagcagg cgctgcccct gttccccgag
     6781 gccaagcggg tgctcctcac cgcctatgcc gacaccgatg ccgccatccg cgccatcaac
     6841 accgcccgcg tcgactatta cctgttgaag ccctgggacc ctccccagga gtcgctttac
     6901 ccggtgctcg acgacctgct ggaggactgg caggcctcct accggccgcc gttcgagggg
     6961 gtccgcgtcc tcggccaccg ctggtcgccc aggtccttcg aggtgcgcga cttcctggcc
     7021 cgccaccagg tgccctatca atggatggac ctggaggcgg acggcgaggc ccggcagctc
     7081 ctggagaccg cctgcggccc cgaggctccc accgcccgcc tgccggtagt catcttcccc
     7141 gacggcagcc ccctgcagga gccgtcgcgc ggcgagatgg ccgagcgcct gggcctgcgc
     7201 acccaggccg agagccgctt ctacgacctg gtcatcgtcg gcggcggacc gtcggggctg
     7261 gcggcggcgg tctatggcgc ctcggaggga ctgcgcacgc tgatgatcga gggggacgca
     7321 ccgggcggcc aggcggccac cagctcgcgg atcgagaact acctcggctt ccccgcgggg
     7381 ctctccggcg ccgacctcgc ccgccgcgcc gtcgcccagg cgacccgctt cggggtggag
     7441 atcctcaccc cgcaggaggc ggcgggcgtc cgcctcgagg acccgtaccg catcgtcgtc
     7501 ctgggcgacc gctcggaggt gagctgccac gccctcctga tctcgaccgg cgtctcctac
     7561 cggaagctgg aggtccccgg ggccgacaag ctcaccggcg ccggcatcta ctacggcgcc
     7621 gccatgaccg aggcgatgtc gtgccgcgac gaggacgtct acatcgtcgg cggcgccaac
     7681 tcggctgggc aggcggccat gcacttcgcc cggtacgcca gcaaggtggt gctgctggtg
     7741 cgcggcggct cgctcgccaa gagcatgtcc cagtacctga tcgaccagat ctccgagacg
     7801 cccaacatcg aggtcaggac ccggacccac gtggccgagg cgcacgggga ggaccacctg
     7861 gaggagctgg tgctggcgcg cgaggacggg gacgagagcg cccgggagac ggtgcccgcc
     7921 cgctccctct tcgtcttcat cggcgcccag ccgcggaccg gctggctcga aggggtggtg
     7981 gagcgggacg agcacggctt catcctctcc ggcccggacc tgcccaaggg gtcggacggg
     8041 cgccgcccgc gcggctggcc gctcgaccgc gacccgttcc tcctggaagc gagcgtcccc
     8101 ggcatcttcg tggcgggcga cgtccgccac ggctcggtca agcgcgtggc ctcgggtgtg
     8161 ggagaggggg ccatcgccgt gtcgttcatc caccagtacc ggagccggct gtaggagaga
     8221 ggaggcacca tgctgcacga tcccagagaa cagaagatgt tcccccggct cgaaggcgag
     8281 gtcctgcagg agctggtgga gctcgggacc gagctggagc tccaggacgg cgaccgcctg
     8341 ttcagcgaag gggaccgcga gtacgacttc ttcgtcgtgc tggacggcca ggtccggatc
     8401 accaagcggg tggggaagga ggagacgctc ctcgcgatcc acgagcccgg cgagttcaca
     8461 ggcgagatct ccatgctcac cggcggcccc gccatcgcca acggctatgc cgaggggccg
     8521 gtccgggtgc tgcgcctcga tccgaacgcc ttccggcgcc tgatggccga gtgcgcgccc
     8581 gtggcccggg tggtcctcac ggccatggcg gcgcgggcga ccgacgtcga cgcccagctc
     8641 cgccagcagg agaagctcgc ctcgctgggc aagctcgcgg ccggcctcgc ccacgagctg
     8701 aacaacccgg cgtccgcggc gcagcgggcg gcgggccagc tccgcgacgc cgcctgcggg
     8761 ttcctccgct tctccctgga gcgcgaagac cccttgtcgc cggagggccg ccgcttcctg
     8821 tgcgacgtcc atcgcgaggt cctggcctgc gccggcccgt cgtcggcacc cccggcgctc
     8881 gatccggtga cccagagcga ccgcgaggac gagctgacgc agtggctgga ggaccggggc
     8941 gtgcaggacg cctgggacct cgcgccgacc ctcgtctccg ccggcttcga ccgccagagc
     9001 ctggaggagg agacccgcgg cgtgcccgac ggcgagctcg aggaggtggt gacctgggcg
     9061 cgctcgattc tggagctgtc cgccatggtg gaccagatcg agaccgcctc cacccgcgtc
     9121 tcggacctgg tctcgtcggt caagcagtac tcctacatgg accaggcgcc gcgccaggag
     9181 atcgacctcc acgaggggct ggagaacacg ctcaagatcc tggcctacaa gctcaggggg
     9241 atcgaggtgg tacgggagta cgaccgcacc ctgccgcggg tgatcgccta cggcagcgag
     9301 ctgaaccagg tctggaccaa cctcatcgac aacgccgccg acgccctgga cgctgccggc
     9361 aacggcaaga ggcgggtgcg catccgcacc tatcgctgcc aggaggacgc cgtggtggag
     9421 gtgaccgacg acggccccgg catcccggag gaggtccagg gcaacatctt caacgccttc
     9481 ttcaccacca agccgatggg gcaggggacc ggcctgggcc tcgacatcgc ccgccgcgtc
     9541 gtccaggtgc gccacaaggg gaccatccgc ttcgaatccc gcccgggaga gaccaccttc
     9601 gaggtgcgcc tgccgatcgg cggggactca tccggagccg tcgcggcctc gtaaccagag
     9661 gcagcgaggg gatctggtag tctctcgcgg attttggggg gaatcggcgg atgagcgagc
     9721 gtgcagcggt cctggcctgg ctggtggcct cgagccttct ggtgccggcc accggatggg
     9781 cccaggccga ggagaaggcg gaggagaggg agaggaagga aaagcaagag gcggaggagc
     9841 cggagcaggc tccggcgctc caggtctacg aggagatcga ggtcaccggc cgcgcttcgg
     9901 acatggtggg ggtggcggac tcggcgagcg aaggggtgac cggccgggag gacctccagc
     9961 gccgcccggt gatgcgggcc ggcgagctgc tggagacggt gcccggggtc atcatcacgc
    10021 agcacagcgg cggcggcaag gcgaaccagt acttcctgcg cggcttcaac ctcgaccacg
    10081 gcaccgactt ccgggtgacc gtggacggca tcccggtcaa catgccctcc cacggccacg
    10141 gccaggggta cagcgacgtc aacttcctga tccccgaggt ggtggagagc gtccgctaca
    10201 agaagggacc ctactacgcc gatgaggggg acttcgcggc ggccggcgc
//
LOCUS       DASPJI010000462         5938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_596982, whole genome shotgun sequence.
ACCESSION   DASPJI010000462 DASPJI010000000
VERSION     DASPJI010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596982"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VEL74_12610"
     CDS             <1..358
                     /locus_tag="VEL74_12610"
                     /inference="COORDINATES: protein motif:HMM:NF023454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HYG63418.1"
                     /translation="RVRSEMHLVVRALGSRDWEEAAAAIRQDPDDPQTVWDAERFEQA
                     LTPFFEEYGELLFTPEARRHQYTRLEKTGDRVWTVSQTLLDPQGDNLWAAEGEIDLRD
                     RHAAEGPLVRLLRIQP"
     gene            373..1476
                     /locus_tag="VEL74_12615"
     CDS             373..1476
                     /locus_tag="VEL74_12615"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HYG63419.1"
                     /translation="MDLGLHFWIGLQPTLDLSDHDRRLLERLRPAGVVLFRGNFQRDA
                     SYEEWTARLRRLLGDAREAIGRERILVGIDHEGGTVHRPPPPITHYSFAKEWAHQAGD
                     VGKAMGVELRSLGINVDFAPVVDIHSNPDNPVIGPRSFGTTPEAVSAAAGDFLRGLQS
                     TGVLGCPKHFPGHGDAAVDSHHAMPVLDLAPETLRERELAPYRALLTDTRLVMTAHIL
                     FPRLDDRPATLSEPILRGLLRDEIGFTGAVVTDDIGMGAVSKLFDDPDAAPRTLRAGG
                     DLILICDYWTETERALAMAAHLEDALRRGTLDETVLEASRSRVETLLSEAPQHEVEIL
                     PEEVFAHHATLAPTRTAAAVSTRAAQSVSVAER"
     gene            2077..3426
                     /locus_tag="VEL74_12620"
     CDS             2077..3426
                     /locus_tag="VEL74_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63420.1"
                     /translation="MRRAVLRTALAVLLIAPAAHALTLDQTIGGCGGLAPTRTIQANP
                     STYKSLIAGLLPGDRMLLAPGTYTQGLRLHGKNGQPNKCIVIEGPATGTPALFVGSGL
                     WNTVSLRDNSYLVIRNLQLDGKGLAGDGVKAEVESTYTHHITLENLKLVGYGQSDQRV
                     GINTKSRAWNWVIRYVTIERAGTGMYLGKSDGTGEFVNGLIEHNLVSGTLGYNVQIKH
                     QKSRATGIGIPAAGTTVIRHNVFSKANNSLTGTAARPNLLVGHWPLSGAGSTDTYQIY
                     GNLFYQNPGEALFQGEGNIALHDNLFVNHLNRAVRIQRHNNLPRKVDVYRNTIVAKTE
                     GIHLYNGDPAYPQRLTGNVVFAASPLSGGVQSANVIGAYASAVNYLVNPMGTLGTLDL
                     YPKSGQLQSLSFITSFLTGYLDWDKDFNGQVHLPTFRGAYSGDGVNPGWKPALAIKP"
     gene            3500..4402
                     /locus_tag="VEL74_12625"
     CDS             3500..4402
                     /locus_tag="VEL74_12625"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HYG63421.1"
                     /translation="MRHPSDDAHAHPTAGNLARLRDLRDLEVADDQPDVRGWPVHTSD
                     GHRVGEVHDLLVDTSALRVRYLDVEIDLDLILGSPESRHAGRQETIPGGPGDTGIHAE
                     DHPTLSKLSAAGVSGMASVAGMVTGLPPTAVAPMVTGTYGVGAPVAEDGTAVSNPHRS
                     MGEHFIRESLSDEENRLSGEPPAREGVRAHGGRRHILIPIGRARLDREDDRVFVDGLK
                     SEAVLELPEYGHGPVSRELEAEVLQRFDRGYVHSPERDFYSHSLYDQDTFYGARRSRT
                     GEETSTRQEREVREDATLPIRGRS"
     gene            4458..5438
                     /locus_tag="VEL74_12630"
     CDS             4458..5438
                     /locus_tag="VEL74_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYG63422.1"
                     /translation="MLQTPALSVSLDDVRAAHERIRPHVHRTPVMTSRQVDTLTGARL
                     FFKCEVFQRVGAFKARGAFSRVTRLTSEERSRGVVAFSSGNHAQAVALAARELGVAAT
                     IVMPADAPALKIAATRGYGAEVILYDRATESREEIARRIASERGATVIPPFDDDAVIA
                     GQGTLAVELLEDVPDLDVLVTPCGGGGLLSGCAVAGRGLRPGLRLYGVEPEAGDDVKR
                     SLEAGEPVQIPVPATIADSLQTTVPARRTLAHIAALTEGIVTVSDLELRRAMAFLAAR
                     MKLVVEPGGSAAFAAILHGRLPDLAGRKVGVVLSGGNIDPDRFGALISGV"
     gene            5528..>5938
                     /locus_tag="VEL74_12635"
     CDS             5528..>5938
                     /locus_tag="VEL74_12635"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HYG63423.1"
                     /translation="MRHDRDEMHRHPAGAAGAATTMDSSVVPLKDLKDFKVAKGDPDV
                     RGWEVLANDGRRIGKVDELLVDTAAMKVRYLDVDLDRDIRGAMGGESLAGDMKNDHVL
                     IPIGSAQIHEREDRVVVDLLHTLVGGLPSYGHGPV"
BASE COUNT          937 a         2260 c         1916 g          825 t
ORIGIN      
        1 ccgggtgcgc tccgagatgc acctcgtcgt gcgcgccctg gggagccgcg actgggagga
       61 ggccgccgcc gccatccgcc aggacccgga cgatccccag accgtctggg acgccgagcg
      121 cttcgagcag gccctcaccc cgttcttcga ggagtacggc gagctgctct tcacccccga
      181 ggcccgccgc caccagtaca cgcgcctcga gaagaccggc gaccgcgtct ggaccgtgtc
      241 ccagaccctc ctcgatccgc agggggacaa cctctgggcc gccgaggggg agatcgacct
      301 gcgcgaccgc cacgccgccg agggcccgct ggtccgcctg ctgcggatcc agccctgaag
      361 gagttgcact cgatggacct cgggctccac ttctggatcg ggctccagcc caccctcgat
      421 ctctcggatc acgaccgccg gctgctggag cggctgcgtc cggcgggggt ggtcctcttc
      481 cgcggcaact tccaaaggga cgcctcctac gaggagtgga ccgcccggct ccggcgcctc
      541 ctcggggacg cccgcgaggc gatcggccgc gagcgcatcc tcgtcgggat cgaccacgag
      601 gggggcacgg tccaccggcc gccgccgccc atcacccact actcgttcgc caaggaatgg
      661 gcccaccaag cgggagacgt ggggaaggcc atgggggtcg agctgcgctc cctgggaatc
      721 aacgtcgatt tcgcgccggt ggtggacatc cactcgaacc ccgacaaccc ggtgatcggc
      781 ccgcgctcct tcggcacgac gcctgaggcc gtgtccgccg ccgccgggga cttcctgcgc
      841 ggcctccagt ctacgggggt gctcggctgc cccaagcact tccccggcca cggcgacgcc
      901 gccgtcgact cccaccacgc catgccggtc ctggacctcg cgccggagac cctgcgcgaa
      961 agggagctcg ccccctaccg cgccctcctc accgacacgc ggctggtgat gaccgcgcac
     1021 atcctcttcc cgcgcctcga cgaccggccg gcgacgctct ccgagccgat cctccgcggc
     1081 ctgctgcgcg acgagatcgg ctttaccggc gccgtggtca ccgacgacat cggcatggga
     1141 gccgtctcga agctcttcga cgaccctgac gccgcccccc ggaccctccg cgccggcggc
     1201 gacctcatcc tcatctgcga ctactggacg gagaccgaaa gggcgctcgc catggccgcc
     1261 catctggagg acgccctccg ccggggcacc ctggacgaga cggtcctcga agcctcgcgg
     1321 tcgcgggtgg agaccctcct gtccgaagcc ccccagcacg aggtggagat cctgcccgag
     1381 gaggtctttg cccaccacgc gaccctcgcg ccgacgcgca cggcggcagc ggtctcgaca
     1441 cgggccgcgc agagcgtcag cgtagcggag aggtgagggg cgggccctga cggcgagcct
     1501 tggaccgcag ggggtggtgg tgcctccccc ttcggcgaag actcctcagt gcttcggcgg
     1561 cattcctcaa tgcttcgccg cgactcctca agactcaggg atcatcccga agcgctccgg
     1621 aacggcgcct caacgtcgag gggccgttcc tcagtgctcc gccgccgctc cggagcattc
     1681 cgggacggct cctcagtgtt gaggagtcat ccctcagtgc ttcgccgcgg ctcctcaaca
     1741 ctgagggacc acgccggagc cttccgggac ggctcctcgg tgcttcgccg ccgttcctca
     1801 acacttcggg gcggcccctc agtgctacga agtccctgcg gagcgttgag ggtcggcccc
     1861 tcagtgttcc ggcgccgcgg cggagcactc cgccgccact cctcaatgtt gaggaacgat
     1921 cccgaagcgt tgaggaaccg cggcgaagca ctgaggaacg gcggctcacc gtgttgcgat
     1981 ctcccttgac tccggttcaa tcctgggaat aatgcacact tgtctatagt ttgaacacgc
     2041 gttcggcacg acccacaacc cggaggaagt ttgtccatga gaagagccgt gctccggacc
     2101 gccctggcgg ttctcctgat tgcccctgcg gcccacgccc tgaccctcga ccagacgatt
     2161 ggaggctgcg gcggcctggc gcccacgcgc accatccagg ccaacccgtc cacctacaag
     2221 tcgctgatcg cggggctgct gccgggcgac cggatgctgc tcgcccccgg tacctacacc
     2281 cagggcctgc gcctgcacgg caagaacggc cagccgaaca agtgcatcgt catcgagggg
     2341 ccggcgaccg gcaccccggc gttgttcgtc ggcagcggtc tctggaacac ggtgagcctg
     2401 cgggacaaca gctacctggt gatccgcaac ctccagctcg acggcaaggg cctcgccggt
     2461 gacggcgtca aggccgaggt cgagtcgacc tatacccacc acatcacgct ggagaacctg
     2521 aagctcgtgg gctacggcca gtccgaccag cgggtcggga tcaacaccaa gtccagggcc
     2581 tggaactggg tcatccgcta cgtcaccatc gagcgcgccg gcaccggcat gtacctcggc
     2641 aagtccgacg gcacgggcga gttcgtcaac ggcctcatcg agcacaacct ggtctccggc
     2701 acgctgggct acaacgtcca gatcaagcac cagaagagcc gcgcgaccgg catcggcatc
     2761 cccgccgcgg ggaccacggt gatccgccac aatgtgttca gcaaggcgaa caactcgctc
     2821 accggcaccg ccgcccggcc caacctcctg gtcggccatt ggccgctctc cggcgccggc
     2881 tcgacggaca cctaccagat ctacggcaac ctcttctacc agaacccggg cgaggcgctg
     2941 ttccagggcg agggcaacat cgccctccac gacaacctgt ttgtcaacca cctgaaccgc
     3001 gccgtccgca tccagcggca caacaacctg ccgcgcaagg tcgacgtcta ccgcaacacc
     3061 atcgtcgcca agaccgaggg catccacctc tacaacggcg atccggccta cccgcaacgg
     3121 ctgaccggca acgtcgtctt cgccgcctcg ccgctctccg gcggcgtgca gagcgccaac
     3181 gtcatcggcg cctacgcctc ggcggtcaac tacctggtca acccgatggg gacgctgggg
     3241 acgctcgacc tctatcccaa gagcggccag ctccagagcc tctcgttcat caccagcttc
     3301 ctcaccggct acctggattg ggacaaggac ttcaatggcc aggtccacct gcccacgttc
     3361 cgcggcgcct actcgggcga cggcgtgaac cccggttgga agcccgccct cgccatcaag
     3421 ccctaagtcc ttcaggcgac aggctttgag tcctcccggc ccggtccttg caccggaccg
     3481 ggcccgagga ggattcacga tgagacaccc atcggacgac gctcacgccc atccaacggc
     3541 cgggaacctc gcgcggctcc gggatctccg cgatctcgaa gtggctgacg accaaccgga
     3601 cgtccgcggc tggcccgtcc acaccagtga cggccaccgg gtcggagagg tccacgatct
     3661 cctcgtggac acttccgcgc tgagggtccg ctacctggac gtggagatcg acctcgacct
     3721 catcctcggc tcgcccgagt cccggcacgc cgggcgccag gagaccatcc cgggaggccc
     3781 aggcgacacc gggattcacg cggaggatca ccccaccctg tcgaagctca gcgccgccgg
     3841 agtgtcgggc atggccagcg tggccggcat ggtcaccggc ctgccgccga cggcggtggc
     3901 ccccatggtc accggcacct acggagtcgg cgcgccggtc gccgaggacg gcacggccgt
     3961 ctccaacccc caccgcagca tgggcgagca cttcatccgc gagtcgctgt cggacgagga
     4021 gaaccggctg agcggcgagc ctccggcccg cgagggcgtc cgcgcccatg gcggccggcg
     4081 gcacatcctg atccccatcg gccgcgcccg cctggaccgg gaggacgacc gggtcttcgt
     4141 cgacggcctc aagagcgagg cggtcctcga gctccccgaa tacgggcatg gcccggtcag
     4201 ccgcgagctc gaggcggagg tcctccagcg cttcgaccgc ggctacgtcc actcgcccga
     4261 gcgggacttc tactcgcaca gcctctacga ccaggacacc ttctacggcg cccggcgaag
     4321 ccggaccggc gaagagacct cgacgaggca ggagcgggag gtccgcgagg acgccaccct
     4381 gcccatccgc ggccggtcct agcgagcagg agagtagggt gcgcgcggcg caccctactc
     4441 gtatacagtc tcccccgatg ctccagaccc cggccctctc cgtcagtctc gacgacgtcc
     4501 gcgccgccca cgagcggatc cgtccccatg tccaccgcac gccggtgatg acctcccggc
     4561 aggtcgatac cttgaccggc gcccggctct tcttcaagtg cgaggtcttc cagcgcgtcg
     4621 gcgccttcaa ggcccgcggc gccttctccc gcgtgacccg gctcacctcc gaggagcggt
     4681 cgcggggcgt cgtcgccttc tcgtcgggca accacgccca ggcggtggcc ctggccgccc
     4741 gggagctggg ggtcgccgcc accatcgtca tgcccgccga cgcgccggcg ctcaagatcg
     4801 cggccacccg cggctacggc gccgaggtga tcctctacga ccgcgccacc gagagccgcg
     4861 aagagatcgc ccgccgcatc gccagcgagc ggggcgccac cgtcatcccc ccgttcgacg
     4921 acgacgcggt catcgccggc caggggacgc tcgccgtgga gctgctcgag gacgtgccgg
     4981 acctggacgt gctcgtcacc ccctgcggcg gcggcggcct gctctcgggc tgcgccgtgg
     5041 ccggccgggg cctgcggccg ggcctgcgcc tctacggcgt cgagcccgag gccggggacg
     5101 acgtgaagcg gtccctggaa gccggcgagc cggtccagat cccggtcccg gccaccatcg
     5161 ccgacagcct gcagacgacg gtgccggcgc gccgcaccct ggcccacatc gccgcgctca
     5221 ccgagggcat cgtgacggtc agcgacctcg agctgcggcg cgccatggcc ttcctcgccg
     5281 cgcggatgaa gctggtggtg gagcccgggg gctcggccgc cttcgccgcg atcctccatg
     5341 gcaggctgcc ggacctggcg ggccgcaagg tgggggtcgt cctctccggc ggcaatatcg
     5401 accccgaccg cttcggcgcc ctgatctccg gggtctgacc tccgctccgg gggcggaccg
     5461 gcccgcgaat tgcacccctc ctctgtcagt gcggcggacc cgccgccagc aacagaggag
     5521 gtacatcatg aggcacgaca gagacgagat gcaccgccat ccggcgggag cggcgggggc
     5581 cgccaccacc atggacagca gcgtggtccc gctcaaggac ctcaaggatt tcaaggttgc
     5641 gaagggggac ccggacgtcc gcggctggga ggtgctggcc aacgacggcc gccgcatcgg
     5701 caaggtggac gagctcctgg tcgacaccgc cgccatgaag gtgcgctatc tggacgtcga
     5761 cctcgaccgg gacatccggg gcgccatggg cggcgagagc ctggccggcg acatgaagaa
     5821 cgaccatgtc ctgatcccca tcggctcggc gcagatccac gagcgcgagg accgcgtggt
     5881 ggtcgacctg ctccacaccc tggtgggggg gctgccctcc tacggccacg gcccggtc
//
LOCUS       DASPJI010000463         3215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_323295, whole genome shotgun sequence.
ACCESSION   DASPJI010000463 DASPJI010000000
VERSION     DASPJI010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323295"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3215
                     /locus_tag="VEL74_12640"
     CDS             <1..>3215
                     /locus_tag="VEL74_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG63424.1"
                     /translation="GYGPAEGATISTWHRVRDLPPGAVMVPIGRPVANTSVHVMDRRQ
                     DAAPPGAVGELWIGGEGLARGYLNRPDLTAERFVPHPWAAGERLYRTGDLVRQRGDGT
                     LEFVGRRDDQVKIRGFRIEPAEIEAVLAGHPEVRECAVLARQDVSGDRRLVAYVAGAE
                     GRPPRAEDLRRYLRSRLPEFMIPSAFVFLDGLPLTPNGKLDRKALPAPRRRSTAGYAA
                     PADAVEQLLEGIWTEVLGLEGVGVHDDFFELGGHSLLATQVASRMRHALLVDLPLRQL
                     FETPTIAGLAAAVRAAMRVTAMAGREAPPIVPLPREGGGGVFPLSFAQQRLWFLDRLE
                     PGGTAYNIPLAVRLGGAIRIPELSWVFAAIVRRHEALRTTFGTRDGQAVQVIAPFSAP
                     PLALVDLAGLPETQRESQAQTLALAEIRRPYDLQRGPLLRLCLVRLDERDHVLLMTLH
                     HIVADGWSMGVLLREIAALHDACERGRAAVLPELPVQYADFAHWQREWLRGEVLEEQL
                     AYWRRQLAGAPRVLDLPLDRPRPARQTFQGAVRSVALAPDLSAALHRLCRRQEATPFM
                     VLLAAWGLLLGRHANQQDVLVGAPIAGRNRQEIENLIGFFVNTLTLRVDWRGAPAFAE
                     LVARLRVAALDGYTHQDVPFERLVDELVSERGLAVSPLFQAVLALQNAGGPAGGEEDL
                     AGTGLALAPLALDLGVAKFDLTLTLVEREGQGFSGTLEYNTSLFDPATIDRLLDRLAA
                     LLAGIVADPAARVLEIPLLAEAERRQLLDWSGAGDLYSPAGSLAERFEAWAAARPDAV
                     AVVFDGAALTYGGLDRRANRLAHRLLDAGVEPGSRVCLAVERGLGLTAGILGILKAGC
                     AYVPLDPAYPRERLAWVLEDAGAAALVTEAETLERLPAWPGRVLLLGAAGGLEARPRV
                     AAAPEWPAYVIYTSGSTGRPKGVVVSHGNVLRLMASTEEWFGFGVEDVWTLFHSFAFD
                     FSVWELWGALLYGGRLTVVPYMVSRSPEAMLELVEREAVTVLNQTPSAFKAFQHADAQ
                     RQEAGARSLRWVIFGGEALEPRGLEGWWRRRERWGTAAPGL"
BASE COUNT          374 a         1131 c         1232 g          478 t
ORIGIN      
        1 cggctacggc cccgccgagg gggcgacgat ctccacctgg catcgggtgc gggatctccc
       61 gccgggtgcg gtgatggtac ccatcggccg tccggtggcg aacacgtcgg tgcacgtcat
      121 ggaccgccgc caggacgccg ccccgccggg cgcggtgggc gagctctgga tcggcggtga
      181 agggttggcc cgcggctatc tgaaccggcc ggatctcacc gccgagcgct tcgtccccca
      241 cccctgggcg gcgggggagc gcctgtaccg caccggcgat ctggtgcggc agcgcggcga
      301 cggcaccctc gagttcgtcg gccgccggga cgaccaggtc aagatccgcg gcttccgcat
      361 cgagcccgcg gagatcgagg ccgtgctggc gggccacccc gaggtccggg agtgcgccgt
      421 cctggcgcgg caggacgtgt ccggagaccg ccgcctggtc gcctacgtcg cgggcgcgga
      481 gggccggccg ccgcgcgccg aggacctgcg gcgctacctg cgctcccgcc tgcccgagtt
      541 catgatcccg tcggcgttcg tgttcctgga cgggctgccg ctgacgccga acggcaagct
      601 ggaccgcaag gcgctgcccg cgccgcggcg ccgtagcacg gccgggtacg ccgcgcccgc
      661 ggatgcggtg gagcagctgc tggaggggat ctggaccgag gtcctggggc tggagggcgt
      721 cggcgtccac gacgatttct tcgagctcgg tggtcactcg ctgctcgcaa cccaggtggc
      781 gtcgcggatg cgccatgcgc tcctggtgga cctgccgctg cgccagctct tcgagacgcc
      841 gaccatcgcg ggcttggccg ccgcggtgcg ggccgcgatg cgggtcactg caatggcagg
      901 ccgggaggca ccgccgatcg tgccgctccc ccgggagggc gggggagggg tctttccgct
      961 gtcgttcgcc cagcagaggc tgtggttcct ggaccgcctc gagccgggcg gcacggccta
     1021 caacatcccc ctggccgtgc gtctgggcgg cgccatccgg atccccgagc tgtcatgggt
     1081 cttcgccgcc atcgtccggc ggcacgaggc gctccgcacc accttcggca cccgcgacgg
     1141 gcaggcggtg caggtcatcg cgccattctc cgctccgcct ctggcgctcg tcgacctcgc
     1201 gggcctgccg gagacacaaa gagagtcgca ggcgcagacc ctcgctctgg cggagatccg
     1261 gcgcccctac gacctgcagc gcgggccatt gctgcgcctg tgtctggtgc gcctggacga
     1321 gcgggaccac gtgctgctga tgaccctcca ccacatcgtc gcggacggct ggtctatggg
     1381 tgtcctgctg cgcgagatcg cggccctcca cgacgcctgc gagagggggc gggccgccgt
     1441 cctccctgag ctgcccgtgc agtacgccga cttcgcccac tggcagcggg agtggctgcg
     1501 gggagaggtc ctggaggagc agctcgcgta ctggcggcgg caactagccg gcgcgccgcg
     1561 ggtgctggac ctgcctctgg accgcccgcg ccccgcccgc cagaccttcc agggggctgt
     1621 gcggagcgtc gccctggccc cggacctgtc ggcggcgttg caccggctct gccggcgcca
     1681 ggaggcgaca ccgttcatgg tcctgctggc cgcctggggg ctcctgctcg ggcgccatgc
     1741 caaccagcag gacgtgctgg tgggagctcc catcgccggc cgcaaccggc aggagataga
     1801 gaacctcatc ggcttcttcg tcaacaccct caccttgcgg gtcgactggc gtggagcccc
     1861 tgccttcgcc gagctggtgg cccggttgcg cgtcgcggcc ctcgacggtt acacccatca
     1921 ggacgtgccc ttcgagcgcc tggtggacga gctggtcagc gagcgcggcc tggccgtatc
     1981 gccgctgttc caggcggtgc tcgcgctcca gaacgccggc gggcccgctg gcggcgagga
     2041 ggacctggcg gggaccgggc tggcgctggc gccgctcgcc ttggacctgg gggtggcgaa
     2101 gttcgacctc acgctgaccc tcgtggaacg ggaaggccag ggcttctccg gcaccctgga
     2161 gtacaacacc agcctcttcg acccggcgac catcgaccgc ctgctggacc gtctcgcagc
     2221 gctgctggcg gggatcgtgg ccgatccggc ggcgcgggtc ctggagatcc cgctgctcgc
     2281 cgaggcggag cgccgccagc tcctggattg gagcggcgcc ggcgatctct attcgccagc
     2341 cggttccctg gcggagcggt tcgaagcctg ggcggcggcg cggccggacg cggtcgcggt
     2401 ggtgttcgac ggcgcggcgc tgacctacgg cgggctcgac cggcgggcca accgcctggc
     2461 gcaccggctg ctcgacgcgg gggtcgagcc cggctcgcgt gtctgcctgg cggtggagcg
     2521 cgggctcggc ctcaccgccg gcatcctcgg gatcctgaag gcgggctgcg cctacgtgcc
     2581 gctcgatcca gcgtacccgc gggagcggct cgcctgggtg ctggaggatg ccggcgcggc
     2641 ggccctggtg accgaggcgg agacgctcga acgactgccc gcctggccgg gccgggtgct
     2701 gctgctcggc gcggcggggg ggctggaggc gcgcccgcgg gtggcggccg cgccggagtg
     2761 gccggcctac gtcatctaca cctcgggctc caccgggcgg ccgaagggcg tggtggtgag
     2821 ccacggcaac gtgctgcggc tgatggcctc gaccgaagag tggttcggct tcggcgtgga
     2881 ggacgtgtgg acgctgttcc actccttcgc cttcgacttc tcggtatggg agctctgggg
     2941 ggcgctgctc tacggcggcc ggctcacggt ggtcccctac atggtgagcc gctcgccgga
     3001 ggcgatgctg gagctggtgg agcgggaggc ggtgaccgtc ttgaaccaga cgccatcggc
     3061 cttcaaggcc ttccagcacg ccgatgctca acggcaagaa gccggcgcgc ggagcctgcg
     3121 ctgggtgatt ttcggcggcg aggcgctgga gccgcgcggc ctggaaggct ggtggcggcg
     3181 gcgtgagagg tggggaaccg cggctcccgg cctgg
//
LOCUS       DASPJI010000464         6624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_712932, whole genome shotgun sequence.
ACCESSION   DASPJI010000464 DASPJI010000000
VERSION     DASPJI010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712932"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VEL74_12645"
     CDS             <1..464
                     /locus_tag="VEL74_12645"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYG63425.1"
                     /translation="DRAAGMALGLSRYTPRLRVLATADPNLSDHRAEQLRQKGIPVLT
                     GTIRRLVGEKRHLTALELEDGTRLEAEAFFVSSPALGRTDLAVQLGVEMAATGHHAQP
                     KSQRGDTNVPGVWIAGDLRPMTQQVAVAMGTGNIAAVMIDQHLRKQDVLGS"
     gene            complement(499..2307)
                     /locus_tag="VEL74_12650"
     CDS             complement(499..2307)
                     /locus_tag="VEL74_12650"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYG63426.1"
                     /translation="MNRIKTILFLAALAGTLASVAASADAAAPDPAIAITNARIFDGE
                     RVIPRGTVVVRGREIAAVGADVKVPAGAEVVDGAGATLLPGLIDSHTHTWGDALSRAL
                     VFGVTTELDMFTSPELARAMREEQAKTGAFERADLFSAGVLATAPGGHGSQFGVPIPT
                     LERPEQAGEFVDARVAEGSDYIKIVSEDGSLYRMSLKTLDTPTIAALIEAAHRRGKLA
                     VVHASTQERAKAALASGADGLVHIFHDRAPEPEFVQLAKGRFVIPTLTVTESATGTAS
                     GRSLNEDPRLKPYLTAEESGNLIRSFPPRDGAAKELGYSLEAVRQLKAAGVPILAGSD
                     APNPGTSHGASIHREMELLVQAGLTPKEALAAATSTPARVFGLKDRGRIAPSLRADLV
                     LVKGDPSTDINATRDILRVWKLGREAQRAKAPAGAPAKAPQAQVPASGEIAGFEDGTA
                     SAAFGFGWADSTDKIAGGKSEVRKEVVEAAGGGKVLAITGEVRAGFAFPWAGAVFLSG
                     PRPMAPVDLSRFAGISFKARGEGEAHQFMVFATRLGQIPVQRPLKAGPEWQTYTFDFA
                     DLNLDGSDVTGFFFGGGPAPGPFRLEIDDVRLVAKK"
     gene            complement(2464..2793)
                     /locus_tag="VEL74_12655"
     CDS             complement(2464..2793)
                     /locus_tag="VEL74_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63427.1"
                     /translation="MKVLVCTLLFSFLFVAGSMAAEPEPAAPPGPATDAAFFCSLAVT
                     PEVPVTKAPITAPVWQEKAVDYDCERLADDCLQAQCQCNEDCNGSVAQMPCVYYPRSW
                     TSGCLCA"
     gene            complement(3072..3392)
                     /locus_tag="VEL74_12660"
     CDS             complement(3072..3392)
                     /locus_tag="VEL74_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63428.1"
                     /translation="MSRYCASSAPDGEVTRIAVRPSRSTWSTRTLADAWGSPSKSSRA
                     SRYQTEVVKVVSQLVRACAFWTSATARERSFRWSQVFFRLTAQVQERIGFALPAESPG
                     TWAR"
     gene            complement(3406..4824)
                     /locus_tag="VEL74_12665"
     CDS             complement(3406..4824)
                     /locus_tag="VEL74_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63429.1"
                     /translation="MRRQAPLRAMVHDRLEPRGVAADLRPPVGDDAGGAGDQERRGRH
                     PLLDEAGHDSHGLEGLAQAHVVAQQAAEPVLPEEAQPFGADPLIGPERRPDPVRLAKR
                     LGRPQLQQALEDRGAVSTVAAAVSGNPHRPFRDRLQPQQLACREADPLLPGVVTDELL
                     QVPERRGVEVYPAAGDLEVGEALLLGVQDLGQRHPLAAEAVLDGEPPPAPLQRLLEVQ
                     LRGGAGDLDRVIGLEDLAVAQRRVEDEHPFADGAGVVVEEARHLMPAQGQAGGGAELP
                     LVALPLQLGQHPQVLEPDEAAVPVLLDLGHLEELLLAGGLEGADHRPDLDGVQLGRLA
                     DVEDGAQGDGADRQVLLVRPGLGEERQGGLHAVTRHGDLDHRLATVDPPPEGEGEGAG
                     GLVDGGVEAAGLLRLQLADAVIGEPVPEALLPGVAVRLHPCLEEKALLRRELKEDEPF
                     QQLGAVEDAGAERHLAGALQEA"
     gene            5063..5407
                     /locus_tag="VEL74_12670"
                     /pseudo
     CDS             5063..5407
                     /locus_tag="VEL74_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
     gene            5426..5974
                     /locus_tag="VEL74_12675"
     CDS             5426..5974
                     /locus_tag="VEL74_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HYG63430.1"
                     /translation="MNQLDRADAYTSGKWELFKEILEEALGSGQKVVVFTQYLGMIEL
                     MRRHLESAGVGHAILTGATTDRGGVVDRFNRDPGCRVFLGSLKAGGTGIDLVGGSVVI
                     HYDRWWNAAREDQATDRVYRLGQKRAVQVFKLVTEGTLEEKISALIDRKRRMMESVVQ
                     EDDPKLAKIFTRDELLEMLRGE"
     gene            complement(6033..>6624)
                     /locus_tag="VEL74_12680"
     CDS             complement(6033..>6624)
                     /locus_tag="VEL74_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63431.1"
                     /translation="TVVERLDDAVLDAKGTGVTRLQFRALSLVSIAPVKTSCGAFHAY
                     VSLAGEQRVTTMRIHRTEKGGGNFVAPLAADVRLTFIPVKPARNKAARKLELTGSFTF
                     PASPIPWSLTDGAMAKGLDAVVVDTNGDLTPDTRLPGASSFLPGLTPKAFEMNKLIED
                     GECCSERICHTDPATGKIHCTMYIPPGCPFAIRCTL"
BASE COUNT         1030 a         2339 c         2274 g          981 t
ORIGIN      
        1 ccgaccgcgc cgccggcatg gccctgggcc tctcccgcta cacccctcgc ctgcgcgtgc
       61 tcgccaccgc ggaccccaac ctctccgacc accgggccga gcagctccgg cagaagggaa
      121 tcccggtgct caccgggacg atccgccggc tagtcgggga gaagcgtcac ctcaccgcgc
      181 tggagctgga ggacggcacg cggctcgagg cggaggcctt cttcgtctcg agcccggccc
      241 tgggccgtac cgacctcgcc gtccagctcg gcgtcgagat ggccgccacc ggccaccacg
      301 cccagccgaa gtcccagcgg ggggatacca acgtgcccgg cgtctggatc gccggcgacc
      361 tgcgccccat gacccagcag gtggccgtcg ccatgggcac cggcaacatc gccgccgtca
      421 tgatcgacca gcacctgcgg aagcaggacg tgctgggctc ctgagagtcc agcacgcgct
      481 cgagctactt cttcggcgct acttcttcgc caccagcctc acgtcgtcga tctccagccg
      541 gaaggggcct ggggccgggc cgccgccgaa gaagaagccg gtgacgtcgc tgccgtcgag
      601 gttgaggtcc gcgaagtcga aggtgtaggt ctgccactcg gggccggcct tcaggggccg
      661 ctgcaccggg atctggccca gccgcgtggc gaagaccatg aactggtggg cctccccctc
      721 gccgcgagcc ttgaacgaga tcccggcgaa ccgggagagg tccaccggcg ccatcggccg
      781 cgggccggac aggaagaccg ccccggccca ggggaaggcg aagccggcgc gcacctcgcc
      841 ggtgatggcg agcaccttgc ctccgcccgc agcctcgacg acctccttgc gcacctccga
      901 cttgccgccc gcgatcttgt ccgtcgagtc cgcccagccg aagccgaagg cggctgaggc
      961 ggtgccgtcc tcgaagccgg cgatctcccc ggaggcgggt acctgcgcct ggggggcctt
     1021 cgccggagcg ccggcggggg ccttggcgcg ctgcgcctcg cggccgagct tccagacccg
     1081 caggatgtcc cgcgtcgcgt tgatgtccgt ggagggatcc cccttcacca gcaccaggtc
     1141 ggcgcgcagg ctgggggcga tgcgcccacg gtccttcaac ccgaacaccc gcgccggcgt
     1201 cgatgtggcc gccgccaagg cctccttggg ggtgaggccc gcctgcacca gcagctccat
     1261 ctcgcggtga atcgaggcgc cgtgcgacgt cccgggattg ggggcgtcgc tgccggcgag
     1321 gatcggcacg ccggcagcct tcaactgccg gaccgcctcc agggagtagc ccagctcctt
     1381 ggccgcaccg tcgcgcggcg ggaaggagcg gatgaggttc ccgctctcct ccgccgtgag
     1441 atacggcttc agccgcggat cctcattcag cgaccggccg ctcgccgtgc cggtggcgct
     1501 ctccgtcacc gtcagggtgg gaatgacgaa ccgccccttg gcgagttgga cgaattccgg
     1561 ctccggagcc cggtcatgaa agatgtggac gaggccgtcc gctcccgacg cgagcgccgc
     1621 cttggcgcgc tcctgggtgc tggcgtggac cacggcgagc ttgccgcgac ggtgcgccgc
     1681 ctcgatcaat gccgcgatgg tgggtgtgtc cagggtcttg agggacatcc ggtagagcga
     1741 gccgtcctcg gagacgatct tgatgtagtc cgagccctcg gcgacccggg cgtcgacgaa
     1801 ctccccggcc tgctccggcc gctcgagcgt cgggatgggg acgccgaact gcgatccgtg
     1861 gccgccgggg gccgtggcga gcaccccggc gctgaacagg tccgcccgct cgaacgcccc
     1921 ggtcttcgcc tgctcctcgc gcatcgcccg ggcgagctcg ggggacgtga acatgtccag
     1981 ctcggtggtg acgccgaaga cgagcgcccg cgacagggca tcgccccagg tgtgggtgtg
     2041 gctgtcgatc agccctggca gcagcgtcgc gccggcaccg tcgacaacct ccgcgccggc
     2101 cggcaccttg acgtcggcgc cgacggcggc gatctcgcgt ccgcgcacca cgacagttcc
     2161 ccgcgggatc acccgctcgc cgtcgaagat ccgggcgttg gtgatggcga tggccgggtc
     2221 cggagccgcg gcatcggcgc tggcggccac actggcgaga gtcccggcca gggctgccag
     2281 gaacaggatg gtcttgattc ggttcatgga tctctcctcc taggccctga gtgtcgcctc
     2341 cgggcggagg accccaaagt ctcgcctgtc actacttgag ggtgacgttt gtcacggggg
     2401 cggtgagccg aagcgggcgg aggagggggg ggacgtgccc ggggcacgtc cccgaacaga
     2461 tcctcaggcg caaagacagc cggaggtcca ggatctcgga tagtagacgc aaggcatctg
     2521 cgcgaccgag ccgttgcagt cttcattgca ctgacattga gcttgcaggc aatcgtcggc
     2581 aagcctctcg cagtcgtagt cgacagcctt ttcctgccag accggggcgg tgatgggtgc
     2641 cttcgtcacc ggcacctcgg gcgtgacggc cagggagcag aagaacgccg catccgtggc
     2701 cggacctggc ggggccgccg gctcgggctc cgccgccatc gatcccgcca cgaagaggaa
     2761 ggagaagagc agggtacaga ccaggacttt catcgcagcc tccttgttgt cgaagcgaaa
     2821 ccccggatga ggcttcgcgg ccagccaggc acatcgtagt ttagaaattc taacatgtct
     2881 tgcatgagcc cctgccggac cttccccctc cccgaagcgc ccgccctgtt ctagaatccc
     2941 ccctcccccg cgacctcgtc cggaaactgc ttctccgtgg aagaaatcat gccgccgttc
     3001 acccatctcg aattccaccg ccagggaatc ggtctcgttc cccacgaacc cggagagacc
     3061 gacaccgcca tctatctcgc ccatgtgccg ggcgactcgg cgggcagtgc gaagccgatg
     3121 cgctcctgca cctgcgcggt cagccggaaa aagacctgcg accatctgaa ggacctctcc
     3181 cgcgccgtgg ccgaggtcca gaaggcgcac gcccggacga gctgggagac gaccttcacc
     3241 acctcggtct ggtaccggct cgcccggctg ctcttcgagg gagagcccca ggcgtccgcc
     3301 aaggtccgtg tgctccaggt ggagcgcgac ggccgcaccg cgatccgcgt cacctcgccg
     3361 tccggcgcgg agctggcgca gtacctggac aactcggagg ggcgcctacg cttcctggag
     3421 cgcaccggca aggtggcgct cggcgccggc gtcttcgacc gcgccgagct gctggaacgg
     3481 ctcgtcctct ttcagctcac gccgcaggag cgccttctcc tcaaggcagg gatgaagacg
     3541 caccgccacg cctgggagga gagcttctgg caccggctcg cctatcactg cgtccgcgag
     3601 ctggaggcgg aggagtccgg cagcttccac cccgccatcg acgaggccac cggcgccttc
     3661 accctcacct tccggcgggg gatcgacggt ggcgagccga tggtccaggt caccgtgccg
     3721 cgtgaccgcg tgcaggcctc cctggcgctc ctcgccgagg cctggccgga ccaggaggac
     3781 ctggcgatcc gccccatccc cctgcgcacc atcttccacg tcggcgagac gaccgagttg
     3841 gacgccgtcg aggtccggcc ggtgatccgc gccctccagg ccaccggcga ggagaagttc
     3901 ctccagatgg ccgaggtcga gaagtaccgg tacggccgcc tcatctggct caaggacctg
     3961 cgggtgctgg ccgagctgga gcgggagggc cacgagagga agttccgcgc ccccgcccgc
     4021 ctgaccctgg gccggcatca ggtgccgggc ttcctcgacg acaacgccgg cgccatcggc
     4081 gaaggggtgc tcgtcctcga cgcgccgctg cgcgaccgca agatcttcaa gacctatgac
     4141 gcgatcgaga tcgccggcgc caccccggag ctggacctcg aggaggcgct ggagcggagc
     4201 tggtggtggc tcgccatcga gtacggcttc ggcagccagc gggtgtcgct ggccgagatc
     4261 ctggacgcca agaagcaggg cctcccctac ctcgagatct ccggcggctg gatagacctc
     4321 cacgccccgg cgttccggca cctggaggag ctcgtcggtc acgacgcccg ggagcagcgg
     4381 gtccgcctct cggcaagcga gctgctgcgg ctgcaggcgg tctcggaagg gccggtgcgg
     4441 attgccggag acggcggcgg cgacggtgga gacggcgccg cgatcctcca gcgcttgctg
     4501 gagctgcggc cggccgagtc gtttcgccag ccggacgggg tctggacgac gctccggccc
     4561 tatcagcgga tcggcgccga atggctgcgc ttcctctggg agaaccggct cggcggcctg
     4621 ctgtgcgacg acatgggcct gggcaagacc ctccagacca tggctgtcat gaccggcctc
     4681 gtcgaggagg gggtggcgac cacgccgttc ctggtcgcct gccccaccag cgtcatcccc
     4741 cactggcggg cgcagatccg ccgccacgcc ccggggctcc agccggtcgt gcaccatggc
     4801 gcgcagcggc gcctgccgtc gcacctcgtg gccggcgacg ttctgattac ctcgtacggc
     4861 gtgctgcgcc gcgacgccga ggcgctgggc gccgtcccct tcgcgctcgc cgtcttcgac
     4921 gaggtccagc aggtgaagaa ccggcagacc caggcctggc aggcggcctc ccgcgtccag
     4981 gcccaggtgc ggatcggcct caccggaaca ccgatcgaga tcgaacagcg tcttcaggtc
     5041 cgccagctcg ttcgatctcg tcctccccgg ctacctgggg agcgacgcgt ccttcgagga
     5101 gcgctacctg cggcccgccg aagggaggaa tcgcgccgcg gcggccgaag aggagcggcg
     5161 gcgggacctc ggccgggccg tggcgccgtt cgtcctgcgg cgcctcaagt cggccgttct
     5221 cgacgagctg ccggagaaga tcgaggacct ccgcacctgt gccctctcgg acgatcaggt
     5281 cagcctctac cgcgacgcga tcagcggcaa gggggccgag ctggcgcggc gcatcgagga
     5341 gtcgccggac gagccggtgc cgtacatcca cgtcttcgcg ctgctcaacc tgctgaagca
     5401 gatctgcgac caccccgccc tggcgctgaa ccagctcgac cgggcggacg cctacacctc
     5461 cggcaagtgg gaactcttca aggagatcct ggaggaggcg ctgggcagcg gccagaaggt
     5521 ggtggtcttc acccaatacc tggggatgat cgagctgatg cggcggcatc tggagagcgc
     5581 cggcgtcggg cacgccatcc tgaccggcgc caccaccgac cgcggcggcg tcgtggaccg
     5641 cttcaaccgc gacccgggct gccgggtctt cctgggcagc ttgaaggccg gcggcaccgg
     5701 catcgacctc gtcggcggct cggtggtcat ccactacgac cgctggtgga acgccgcccg
     5761 cgaggaccag gccaccgacc gcgtctatcg tctggggcag aagcgggcgg tgcaggtctt
     5821 caagctcgtc accgaaggga ctctcgaaga gaagatctcc gccttgatcg accgcaagcg
     5881 ccgcatgatg gagagcgtcg tccaggagga cgaccccaag ctggcgaaga tcttcacccg
     5941 cgacgagctg ctggagatgc tgcgggggga gtgaggaggc gccccccatt tccggggcgc
     6001 cccactcata actggcctct cacacctttt gtttagagcg tgcaacggat cgcaaacggg
     6061 cagccgggag ggatatacat cgtgcagtgg atcttgccgg ttgccggatc ggtgtggcag
     6121 atccgctcgc tgcagcactc cccatcctcg atcagcttgt tcatttcaaa cgcctttggc
     6181 gtcaagcccg gcagaaagct cgaggcgccg ggaagccggg tgtcgggggt caggtcgcca
     6241 ttggtgtcga cgaccacggc atccaagccc ttggccatgg cgccgtcggt gaggctccac
     6301 ggaatgggag aggcagggaa ggtgaagctt cccgtgagct cgagcttccg cgcggccttg
     6361 ttccttgcgg gcttcacggg gatgaaggtc aagcgcacgt cagccgcgag aggcgcgacg
     6421 aagttgccgc cgcccttctc ggtgcgatga atgcgcatcg tcgtcacccg ctgctcgccg
     6481 gccagtgaca cataggcatg gaaagccccg caggaggtct tgaccggggc gatgctcacc
     6541 aaggagagcg cacggaactg gagacgcgtc acgccagtcc ctttagcgtc gagaacggca
     6601 tcgtcgaggc gctcgaccac cgta
//
LOCUS       DASPJI010000465         3262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_257450, whole genome shotgun sequence.
ACCESSION   DASPJI010000465 DASPJI010000000
VERSION     DASPJI010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257450"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..155)
                     /locus_tag="VEL74_12685"
     tRNA            complement(63..155)
                     /locus_tag="VEL74_12685"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Ser,seq:gct)
     gene            312..2336
                     /gene="tkt"
                     /locus_tag="VEL74_12690"
     CDS             312..2336
                     /gene="tkt"
                     /locus_tag="VEL74_12690"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HYG63432.1"
                     /translation="MGEHETQGGDQLAINTIRFLAVDMVEKAASGHPGAPLGQAPLAY
                     LLWTRHMRHNPDNPDWPDRDRFVLSCGHASALLYALLHLGGYGLPMEELQRFRQLGSK
                     TPGHPEHELTRGVETTTGPLGQGIGNSVGMAIAQRMAAAHFNRDGFSLFEHRVWVIAS
                     DGDLMEGVSHEACSMAGHMRLGNLKVFYDDNEISIDGPTALSFSDDAAKRFESYGWNV
                     LRVNDGNDLAALEEAIAAAEAETERPTLVVVRTVIGFGSPKKAGSAEAHGSPLGPDET
                     RATKRNLGWPEDSAFHIPEGALAPFEAARERGRKLEAEWNERLRRYAEAHPEQAADLR
                     RRLEGKLPDGWQEAIPSFKPGEDMATRSASGKVLAGILPKLPELVGGSADLTPSNDTY
                     VKGTAAFAVNNPSGRYFHFGVREHGMGSAMNGIALSRLWIPYGGTFLIFSDYMRPPVR
                     LAALMKLQAIYVYTHDSIFLGEDGPTHQPVSQLPSLRAIPGLTVLRPADACETAEAWR
                     VAIEHRHGPVAIALTRQKVPVLEETAARAREGVGRGAYVLHDPEGGDPEALILATGSE
                     VAVALEAAKQLAGEGGEGIRARVVSMPSWELFDAQPKEYRDQVLPPAVRTRLAVEAAA
                     PFGWHQYVGLDGEVHGIDRFGASAPAKDLAKAFGFTPEAVAKRVRGMLGR"
     gene            complement(2346..2435)
                     /locus_tag="VEL74_12695"
     tRNA            complement(2346..2435)
                     /locus_tag="VEL74_12695"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2399..2401),aa:Ser,seq:tga)
     gene            complement(2454..>3262)
                     /gene="serS"
                     /locus_tag="VEL74_12700"
     CDS             complement(2454..>3262)
                     /gene="serS"
                     /locus_tag="VEL74_12700"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HYG63433.1"
                     /translation="FTVYRGAGARLERALISFMLDLHTGRHGYTEILPPFMVNRETLF
                     GTGQLPKFEQDLFKLEGTPYYLIPTAEVPVTNLHRGETLDEAVLPVRYTAYTPCFRSE
                     AGSHGRDVRGLIRQHQFNKVELVQLTSQETSYNALEELTGHAETVLQLLGLPYRKVCL
                     STGDMGFSAAKTYDLEVWLPGQGAYREISSCSNCEDFQARRANLRYRPAGGGKAKLLH
                     TLNGSGLAVGRTLIAVLENYQEEDGSVVVPEPLRPYMGGLERITASTASG"
BASE COUNT          507 a         1127 c         1165 g          463 t
ORIGIN      
        1 aagcggcgag ggagcgacga ccgaagcccg cccgctccgc ctgagggagc gcaagcgacc
       61 gatggcggag agggggggat tcgaaccccc ggcccgggtt taccccgggc aactgcttag
      121 caggcagccc tgttcggcca ctccagcacc tctccgtgcg gactgcacag ggggagtcat
      181 tctagaggcg gatcgcttcg gagtcaaagg tcggcccttt cccgccctgc cccaccctga
      241 gacagcccgc aggataaact atgcgccccg cccgttcatt cctcgcgggg agggcgaagg
      301 gaggcgaatc tatgggtgaa cacgaaacgc aggggggcga ccagctcgcc atcaacacca
      361 tccgctttct cgccgtggac atggtcgaga aggcggcgtc agggcatccg ggagcgccgc
      421 tgggccaggc gccgctcgcc tatctgctgt ggacacggca catgcggcac aacccggaca
      481 accccgactg gccggaccgc gaccgcttcg tgctctcgtg cggccacgcc tcggccctcc
      541 tctacgcgct gctgcacctg gggggctacg gcctgcccat ggaggagctg cagcgattcc
      601 gccagctcgg ctcgaagacc ccgggccacc ccgagcacga gctgacccgc ggcgtcgaga
      661 ccaccaccgg cccgctcggc caggggatcg gcaactcggt gggcatggcg atcgcccagc
      721 gcatggccgc cgcccacttc aaccgcgacg gcttctccct gttcgagcac cgcgtctggg
      781 tgatcgccag cgacggcgac ctgatggagg gggtctccca cgaggcctgc tcgatggccg
      841 gccacatgcg cctgggcaac ctcaaggtct tctacgacga caacgaaatc tccatcgacg
      901 gccccaccgc cctctccttc tcggacgacg cggccaagcg cttcgagtcc tacggctgga
      961 acgtgctgcg ggtgaacgac ggcaacgacc tcgcggcgct ggaggaggcc atcgccgccg
     1021 ccgaggccga gacggagcgc cccaccctgg tggtggtgcg gacggtcatc ggcttcggca
     1081 gccccaagaa ggcgggatcg gccgaagccc acggctcgcc cctcgggccg gacgagacca
     1141 gggcgaccaa acggaacctc ggctggccgg aggattcggc gttccacatc cccgaggggg
     1201 cgctggcgcc gttcgaggcc gcccgggagc ggggccgcaa gctggaggcg gagtggaacg
     1261 agcggctccg ccgctacgcg gaggcccatc cggagcaggc ggccgacctc cggcgccggc
     1321 tggagggcaa gctgccggac ggctggcagg aggcgatccc gtccttcaag ccaggcgagg
     1381 acatggcgac ccgctcggcg tcgggcaagg tgctggcggg catcctgccg aagctgccgg
     1441 agctggtggg cggctcggcc gacctcacgc cgtccaacga cacctacgtc aaggggacgg
     1501 cggccttcgc ggtgaacaat ccgtccggcc gctacttcca cttcggagtc cgcgagcacg
     1561 gcatgggctc ggcgatgaac ggcatcgccc tgtcgcggct ctggatcccg tacggcggca
     1621 ccttcctgat cttctcggac tacatgcggc cgccggtgcg cctggcggcg ctgatgaagc
     1681 tgcaggcgat ctacgtctac acccacgact ccatcttcct gggcgaggac gggccgaccc
     1741 accagccggt cagccaactc ccttcgctgc gcgccatccc cggtttgacc gtgctccgtc
     1801 cggccgacgc ctgcgagacc gcggaggcct ggcgggtcgc catcgagcac cggcacggtc
     1861 cggtggccat cgccctcacc cgccagaagg tgccggtgct ggaggagacg gcggcccgtg
     1921 cccgcgaggg tgtcggccgc ggcgcctatg tcctccacga cccggagggc ggcgatcccg
     1981 aggcactgat cctcgccacc ggctccgagg tggcggtggc gctggaggcg gcgaagcagc
     2041 tcgccggcga ggggggtgag gggatccgcg cccgggtggt cagcatgccc tcgtgggagc
     2101 tgttcgacgc gcagcccaag gagtaccggg accaagtctt gccgccggcg gtgcggacgc
     2161 gcctggcggt ggaggcggcg gcccccttcg gctggcacca gtacgtcggc ctggacggcg
     2221 aggtccacgg catcgaccgc ttcggcgcct cggcccccgc caaggacctg gcgaaggcgt
     2281 tcggcttcac ccccgaggcg gtggcgaagc gggtgcgggg gatgctgggg aggtaggaaa
     2341 acaattggcg gagggagtgg gattcgaacc cacgggccgc gtgaacggcc ggcggttttc
     2401 aagaccgctg ctttaaacca ctcagccatc cctccggtct tgccgacaac agcttacccc
     2461 gacgcggtcg acgcggtaat cctctccagg ccacccatgt aggggcggag gggctccggg
     2521 accaccaccg agccgtcctc ctcctggtag ttctcgagga cggcgatgag cgtccgcccc
     2581 accgccagac ccgagccgtt gagggtatgg aggagcttcg ccttgcctcc cccggccggc
     2641 cgatagcgga ggttggcccg ccgcgcctgg aagtcctcgc agttgctgca cgaggagatc
     2701 tcgcggtagg ccccctggcc cggcagccag acctcgaggt cgtaggtctt cgccgccgag
     2761 aagcccatgt cgccggtgga gaggcagacc ttgcggtagg ggagcccgag gagctggagc
     2821 accgtctcgg cgtggccggt cagctcctcc agggcgttgt agctcgtctc ctgggaggtg
     2881 agctgcacca gctccacctt gttgaactgg tgctggcgga tcagcccccg tacgtccctg
     2941 ccgtgggagc ccgcctcgct gcggaagcag ggggtgtagg cggtgtagcg gaccggcagg
     3001 acggcctcgt ccagcgtctc gccgcggtgc aggttggtca ccggcacctc ggcggtaggg
     3061 atgaggtagt agggggtccc ctccagtttg aagaggtcct gctcgaactt ggggagctgc
     3121 ccggtgccga acagggtctc gcggttgacc atgaacggcg gcagaatctc cgtgtagccg
     3181 tgccggccgg tgtggaggtc cagcatgaag gagatcaggg cccgctcgag acgggcgccg
     3241 gcgccgcggt agacggtgaa gc
//
LOCUS       DASPJI010000466         2787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_713088, whole genome shotgun sequence.
ACCESSION   DASPJI010000466 DASPJI010000000
VERSION     DASPJI010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713088"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2787
                     /locus_tag="VEL74_12705"
     CDS             <1..>2787
                     /locus_tag="VEL74_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG63434.1"
                     /translation="LTAGKFLPDPWSATPGARLYRTGDRARYRPDGNLEFLGRLDHQV
                     KIRGFRIELGEIEAALATLPGVREAVVVVREDAPGDRRLVAYVTGDAAAADDLRSSLQ
                     ERLPEPMVPAAFVSLAALPLTPNGKVDRKALPAPEWQRAEESHLAPRTPAEEILAGIW
                     AEVLGLDRVGATDHFFELGGHSLLATQVMSRVGEAFGVELPLRELFEAPVLADLAAQV
                     EAARREGVARTAPPLVPVPRGGPLPLSFAQQRLWFIEQFAPGNTLYNIPMALRVEGPL
                     RPEVLALCLGEIARRHEVLRTVFAARDGLPLQVIQAAAPFSLPLVDLSGLPAERREAT
                     ALALAGEEAGRPFDLTGLGRGPLLRAVLLRLQAADHVVAPTLHHIAGDGWSLGVLVRE
                     ITALYAAAVEGRPSPLPELPVQYADYSVWQHAWLRGEVLEGEIAFWRRQLAGLPPLLE
                     LPADRPRPAVQSPRGASEPLRLPAGLARRVQALGRREGATLFMVLLAGFDALLARYSG
                     QPDLAVGTPVAGRNRVEIEGLIGFFVNTLVLRADLSGGPSFRELLARVREAALAAHAH
                     QDVPFEKLVQELAPERSLAHTPLFQVMLSMQSFPSTAIGLPGLSMTPVALETTAVRFD
                     LELTFAEEGDELGARLDFASGLFDRATIRRLAGHLEALLEGATADPDQRLSELPLLAA
                     AEGHQLLAEWNDTGPPLPERVLLHDLVEAQARSTPDAPAVTFDGETLTYAGLHDRADR
                     LARHLRDLGCGAESRAGVALERSLDLIVALLGVLRAGAAYVPLDPGYPRERLALVLED
                     AAPRVLVTRTPLLAALPPFPGEVLCLDGEPIYKDVTARAPVPGDDRQLAYVLYTSGST
                     GRPKGVGVPHRALVSFLRSMQGAPGFAAGERLLAVTPVAFDIAALEIFLPLITGGCVE
                     LAGREEAADGRLLA"
BASE COUNT          307 a         1037 c         1034 g          409 t
ORIGIN      
        1 gctgaccgcc ggcaagttcc tccccgaccc gtggagcgcc acgcccggcg cccggctcta
       61 ccgcacgggc gaccgcgccc gctaccgtcc ggacggcaac ctggagttcc tggggcggct
      121 ggaccatcag gtcaagatcc gcggcttccg catcgagctg ggggagatcg aggcggcgct
      181 cgctaccctt cccggcgtgc gcgaggcggt ggtggtggta cgggaggacg cccccggcga
      241 ccgccggctg gtcgcctacg tgaccggcga cgccgccgcg gcggacgatc tgcgctcttc
      301 cctccaggag cggctgccgg agcccatggt cccggccgcc ttcgtgtctc tggcggcgct
      361 gccgctgacg ccgaacggca aggtggaccg gaaggcgctg cccgctccgg agtggcagcg
      421 cgccgaggag agccatctcg cgccgcgcac cccggcggag gagatcctgg cggggatctg
      481 ggccgaggtc ctggggctcg accgggtggg cgcgaccgat cacttcttcg agctgggcgg
      541 ccactcgctc ctggcgacgc aggtgatgtc acgcgtggga gaggcgttcg gggtcgagct
      601 gccgctacgc gagctgttcg aggcgccggt gctggcggac ctcgcggcgc aggtcgaggc
      661 ggcgcggcgg gagggggtgg cgcggaccgc gccgcctctg gtgccggtgc cgcggggagg
      721 gccgctgccc ctgtccttcg cccagcagcg cctctggttc atcgagcaat tcgcgccggg
      781 caacacgctc tacaacatcc ccatggcgtt gcgggtggag gggccgctcc gccccgaggt
      841 attggccctc tgcctcggcg agatcgcgcg gcggcacgag gtcctgcgca cggtctttgc
      901 cgcgcgagac ggcttgccgc tgcaggtcat ccaggcggcg gcgcccttct ccctccccct
      961 ggtcgacctg tccgggctgc ccgcggagcg tcgggaggcg acggccctcg ccctggccgg
     1021 cgaggaagcg ggccgcccgt tcgatctcac cggtctcgga cgcggtccgc tgctgcgcgc
     1081 cgtgctcctg cggctgcagg cggcggacca cgtcgtcgca cccaccctgc atcacatcgc
     1141 cggcgacggc tggtcgctcg gcgtcctggt gcgcgagatc acggcgctct acgccgcggc
     1201 ggtggagggg aggccctcgc ccttgccgga gctgccggtg cagtacgcgg actactccgt
     1261 ctggcagcac gcctggctgc gcggcgaggt cctggaaggg gagatcgcct tctggcggcg
     1321 gcagctcgcg ggcctgccgc cgctcctcga gctccccgcc gaccgcccgc ggccggcggt
     1381 gcagagcccg cgcggcgcgt ccgagcccct gcggctgccg gccgggctcg cccgccgggt
     1441 gcaggcgctg ggccggcgtg aaggggcgac gctcttcatg gtgctgctgg ccgggttcga
     1501 cgccctcctc gcccgctaca gcggccagcc ggacctcgcg gtgggcacgc cggtcgccgg
     1561 ccgcaaccgc gtcgagatcg aagggctgat cgggttcttc gtcaacacgc tggtgctgcg
     1621 cgccgacctg agcggcgggc cgtcgttccg cgagctgctc gcccgggtgc gggaggccgc
     1681 gctggccgcc cacgcgcatc aggacgtgcc cttcgagaag ctggtccagg agctggcgcc
     1741 ggagaggagc ctcgcccata ccccgctttt ccaggtgatg ctctcgatgc agagcttccc
     1801 gtccacggcg atcgggctgc ccggactctc catgactcct gtcgcactgg agacgacggc
     1861 ggtcaggttc gacctggagc tgacgttcgc ggaggagggg gacgagctgg gcgcccggct
     1921 cgatttcgcg agcggtctct tcgaccgcgc cacgatccgc cgtctggccg gtcatctgga
     1981 ggcgctcctg gaaggagcca cggccgatcc cgaccagcgc ctgtccgagc tgccgctgct
     2041 cgccgccgcc gagggtcacc agctgctggc agagtggaac gacaccgggc cgccgctccc
     2101 ggagcgggtc ctcctgcacg atctcgtgga ggcgcaggcc cggtccacgc cggacgcccc
     2161 ggccgtcacc ttcgacgggg agaccctgac ctatgccggg ctccacgacc gggcggaccg
     2221 gctggcgcgc cacctgcggg atctgggctg cggtgcggag agccgcgccg gcgtcgccct
     2281 cgagcgttcg ctggacctga tcgtcgccct gctcggcgtg ctccgggccg gcgccgccta
     2341 cgtgcccctc gatcccggct acccccggga gcggctggcc ctggtgctgg aagacgccgc
     2401 gccgcgggtg ctcgtcaccc ggacgcctct gctcgccgcc ctccccccgt tccctggaga
     2461 ggtgctctgc ctggacggcg agcctatcta taaggatgtg actgcccgcg cgccggtccc
     2521 aggggacgac cgccagctcg cctacgtcct ctatacctcg ggctccaccg gccgtcccaa
     2581 gggagtcggg gtcccgcacc gggcgctggt gagtttcctg cgctccatgc agggggctcc
     2641 gggcttcgcc gcgggggagc ggctgctcgc cgtcacgccg gtcgccttcg acatcgcggc
     2701 gctggagatc ttcctgccgc tgatcaccgg cggctgcgtc gagctggccg gccgggagga
     2761 ggccgcggac ggccggctcc tcgccga
//
LOCUS       DASPJI010000467         4723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_661767, whole genome shotgun sequence.
ACCESSION   DASPJI010000467 DASPJI010000000
VERSION     DASPJI010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661767"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VEL74_12710"
     CDS             complement(<1..545)
                     /locus_tag="VEL74_12710"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63435.1"
                     /translation="MKSISTLALATSLTLSLAAVSMPAAAQSERSEQRARRLVAELRE
                     DLRPVEDQIRNARFLADLEAGRVSQAQLRAFAGEQYQILINDVRSVAHMVSRFGTEPT
                     GVFFRGNLEGELIARGLFLDFAAALGMTEADLLAYEPRPQGQTYGHYVATMAAYATNA
                     QIAAAFLVNFPVFGENTGRMGA"
     gene            686..1483
                     /locus_tag="VEL74_12715"
     CDS             686..1483
                     /locus_tag="VEL74_12715"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYG63436.1"
                     /translation="MFAVRSQGLLTQLRMARTLFGRPLHDVFAYVFEDLLIDTGPPTT
                     AAQLAAWCRTRPEIRRVLLTHHHEDHVGGAAVLHEELGLPVLAPASALAILAEGLRMP
                     LYRRLVWGTPRPFRAAPLGETVEGEGYRLRVIPTPGHAFTHVCFFEEERRWLFSGDLY
                     VHERVRYLRRIEDVQEHMASLHRVLALEPKLLICDHAGTHPDAGERLAGKIRWWEELA
                     GKARELRDQGLEEAEIARRLLGRDGLLSWVSLGDFSHRNLIRALLSA"
     gene            complement(1490..2335)
                     /locus_tag="VEL74_12720"
     CDS             complement(1490..2335)
                     /locus_tag="VEL74_12720"
                     /inference="COORDINATES: protein motif:HMM:NF039589.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5919 domain-containing protein"
                     /protein_id="HYG63437.1"
                     /translation="MSLVALVAQAFSLSARLTALISLYVVAVIYTWLYRRWINARVSS
                     AGAIAFTLTGVMAAFLLPPGMGLRGGDLSPAGILGYGERANDWAVPKVPEYARAAKDE
                     VWYVGIDFHISSSQHQEMILKLLGEGVDVRFLIYDFLDEEALGINPNFDQLVRRFDCD
                     GAQVIADLTSTVENLRRLQRRWTNKTSGAQLEIRLYRALPWARAYFFDPEQENGTALL
                     VSYIYGQDAHNLPALLLRSHPKGLLPQYFKGAQALWRDSEDFTSWLTAYDAYKARNAV
                     KTTGR"
     gene            2535..2954
                     /locus_tag="VEL74_12725"
     CDS             2535..2954
                     /locus_tag="VEL74_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63438.1"
                     /translation="MTREEFERRKQRLEEQLHTGIELLQAGHRVQIRALEMVWMLAAQ
                     EAETAAAAVPVSRQPARQPEPPPVPRRGLPQVEDDLRAVMEGLPPVFDRSDVIQALGY
                     EPDRRTLYKLLQELTREGTLAVEEPGFGRKPSRYRKV"
     gene            complement(3428..>4723)
                     /locus_tag="VEL74_12730"
     CDS             complement(3428..>4723)
                     /locus_tag="VEL74_12730"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HYG63439.1"
                     /translation="ESRWAPSSVPCAVLSPQEMLLDSREVATLWYLLTGGELDPAGAR
                     ELCEASDGWYRPLRLALDATGGVGLDQPTPEQLLEVSPVRYFLRHEVLHSFSTEERDL
                     LLASSLENDERDDGEAAWHLLESRGLWIEGDQRDRLPRLLAAMLERERQRRGTPKAAP
                     DSSRPTPREPPPRAEASRPVYRLGLLGSPVAQFRDAQGEHPLDWRLKRSFQILAFLGS
                     SPELQATREDLIEAIWPTEGERTIERNFHPTLSHLRRALEGDRKGSGPAPLLFRNGIY
                     RLNPEITWEIDVLDFGRHIETGKELAGRQDSQGAAESLRSAWKLYRGPFLQGHYEAWI
                     TARREEYQRQYLEMLRDLGKLYVGLGKIEEAMDAYRSVLLEDPLQEKIHLEVMLLYAG
                     QGRRDLVRRQYDRLCMLLLEELGVEPMPETTREYHKLMG"
BASE COUNT          840 a         1574 c         1566 g          743 t
ORIGIN      
        1 gcgcccatgc ggccggtgtt ctcgccgaag accgggaagt tcaccaggaa ggcggcggcg
       61 atctgggcgt tggtggcgta ggcagccatc gtggccacgt agtggccgta ggtctgcccc
      121 tgcggccggg gctcgtaggc cagcaggtcc gcctccgtca tgccgagggc cgccgcgaag
      181 tccaggaaca gcccgcgggc gatcagctcc ccctcgaggt tcccccggaa gaagacaccg
      241 gtcggctcgg tgccgaagcg ggacaccatg tgggcgacac tgcgcacgtc gttgatcagg
      301 atctgatact gctcgccggc gaaggcccgg agctgggcct gcgacacccg gccggcctcc
      361 aagtcggcga ggaaccgggc gttgcggatc tggtcctcga cgggccggag gtcctcgcgc
      421 agctcggcga cgagccggcg ggcccgctgc tcggagcgct cggactgggc ggcggcgggc
      481 atggacaccg cggccaggga gagggtcagc gacgtggcca gggccagggt cgagatggat
      541 ttcatgagac ggtcctcctc attggtacga cagatcatgg gtggaacgat acggcggcct
      601 gagtgaccgc ccgaggaacg gcatccaaga taacgcttcc gacccccggc gtccctcacc
      661 ggccacggtt atgatgagag gtccgatgtt cgccgtccgc tcccaaggcc tgctcaccca
      721 gctccgcatg gcgcggaccc tcttcggcag gccgctccac gacgtcttcg cctatgtctt
      781 cgaggatctc ctcatcgaca ccgggccgcc gactacagcc gcgcagttgg ccgcctggtg
      841 ccgcacccgg ccggagatcc gccgggtgct cctcacccac caccacgagg accacgtcgg
      901 gggcgccgcg gtgctccacg aggagctggg cctgccggtg ctcgccccgg ccagcgccct
      961 ggccatcctc gccgaggggc tgcgcatgcc cctttatcgc cgcctggtct gggggacgcc
     1021 gcgccccttc cgcgccgcgc cgctgggtga gaccgtcgag ggagagggct atcgcctccg
     1081 ggtgatcccg acgccggggc acgctttcac ccacgtctgc ttcttcgagg aggagcggcg
     1141 ctggctcttc tccggagacc tctacgtcca tgaaagagtg cgctacctcc ggcgcatcga
     1201 agacgtccag gagcacatgg cgtcgctgca ccgcgtgctg gccctggagc cgaagctcct
     1261 catctgcgac cacgctggca cacaccccga cgccggggaa aggctggcgg ggaagatccg
     1321 ctggtgggag gagctggccg gcaaggcgcg ggagctgcgg gatcaggggc tggaagaggc
     1381 ggagatcgcc cgccggctgc tggggcggga tggcctactg tcctgggtga gcttggggga
     1441 cttctcccac cggaacctga tccgggcgct gctgagcgcc tgaccccgct caacgcccag
     1501 tcgtttttac tgcattccgc gctttgtagg cgtcgtaagc ggtcagccag gacgtgaaat
     1561 cttcagaatc gcgccacaac gcctgcgccc ctttgaagta ttgcggaagg aggcccttgg
     1621 gatgactacg gagcaggaga gctggcaggt tgtgcgcgtc ctggccatag atgtagctga
     1681 cgagaagcgc ggtgccgttc tcttgctcgg gatcgaagaa ataggcccgc gcccagggaa
     1741 gggcgcggta aagtcgaatc tcgagctggg cacctgaggt cttgttcgtc caacggcgct
     1801 gaagcctacg gaggttctcg acggtgctcg tcagatcggc aatgacctgt gcgccatcac
     1861 agtcgaagcg cctcacgagt tggtcaaagt tcggattgat gcccagtgcc tcctcgtcca
     1921 gaaagtcgta gatcaagaag cggacatcga ccccctcgcc caacaacttg agaatcatct
     1981 cctgatgctg agatgagctg atatgaaagt cgatgccgac ataccaaact tcatccttgg
     2041 cggctcgggc atactccggc actttgggta ccgcccaatc attggcacgc tcgccgtagc
     2101 cgaggattcc ggccggagac agatcacctc cacgcagtcc catgcccggc ggcaggagaa
     2161 atgctgccat gacgccggtc aacgtgaagg cgatcgcacc tgcggagctc acgcgcgcgt
     2221 tgatccaacg acggtataac cacgtgtaga tgacggccac cacatacagg gatataagag
     2281 cggtcaggcg ggcgctcaag gagaacgcct gggctacgag cgccaccaga gacacgggaa
     2341 ccagaacctc cccaagctcc ttcaggatgc gaagcgtctt ccggtttgca cgctcaccag
     2401 aggcttgcac tgggctcgca gttgggtccg ccataggctt aacatgatag cgcaactcag
     2461 catccccgac actgccctct ctccctcctt gtgatctaat gatccagatc acaagatcac
     2521 aaggaggcgt tacggtgaca cgagaggagt tcgagcgcag gaagcagagg ctggaggagc
     2581 agctccatac cgggatcgag ctgctgcagg cgggccaccg cgtccagata cgggcgctgg
     2641 agatggtctg gatgctcgcg gcccaggagg ccgagacggc cgcggccgcg gtgccggtct
     2701 ccaggcagcc ggcgcggcaa cccgagccac cgccggtgcc gcgcagaggt ctgcctcagg
     2761 tcgaggatga tctgcgcgcc gtcatggagg gattgccacc cgtcttcgac cgctccgacg
     2821 tgatccaggc ccttggctat gagccggacc gccgcacgct ctacaagctg ctccaggagc
     2881 tgacccgcga gggaacactg gcggtggaag agccgggctt cggacggaag ccgtcccgtt
     2941 accggaaggt ctgagggtaa cgaagagacc tgaccgcgtc attacagccc aacaacgaac
     3001 ggctctagcg tcacaagaga tcactacaga gttctacagg accatttcca ggcttcatcg
     3061 acctcgcgac cacgcgccgg agtcattcca acaggtcgcg gcgtcactac aactcgacgc
     3121 ggaatgacct ccccaactcc catagtgact ccaccagccg gtggaacgct cgctgtacct
     3181 ggagccgtga cgccaccagc tacaggaatg accccacgtc ctacagaaac gttccctgga
     3241 gctgaagaaa tgctcccacc agccggtgga atgaccccac caggtacaga aaagactcca
     3301 acggctcgtg gaatgacctc ttgaggagct gcaacgcccc tgccggctga aggaatgatc
     3361 tcggaacccg gggaaatgac gtctgcaccg ggatgaacag tccggcagga ggggcgaccg
     3421 ccgcccccta ccccatcagc ttgtgatact cgcgggtggt ttccggcatg ggctcgacgc
     3481 ccagctcttc gaggaggagc atacagaggc ggtcgtactg acggcgtacc agatcgcggc
     3541 ggccctggcc cgcgtagagg agcatcacct ccaggtggat cttctcctgg agcgggtctt
     3601 ccaggagcac cgagcggtag gcgtccatcg cctcctcgat cttccccagt ccgacgtaga
     3661 gctttcccag gtcccgcagc atctccaggt actggcgctg gtattcctcc cggcgggccg
     3721 tgatccaggc ttcgtagtgc ccctggagga aggggccgcg gtagagcttc caggcggatc
     3781 gcaaggactc ggccgcccct tgggagtcct gacggcccgc cagctcctta ccggtctcga
     3841 tgtgccggcc gaagtccagc acgtcgatct cccaggtgat ttccggattc aaccggtaga
     3901 tgccgttgcg gaacaggagc ggcgccggac cgctcccctt gcggtccccc tccagggcgc
     3961 ggcgaagatg gctgagcgtc gggtggaaat tgcgctcgat agtgcgctcc ccctccgtcg
     4021 gccagatcgc ctcgatgaga tcctcccggg tcgcctggag ctcgggcgag gaccccagga
     4081 aggcgaggat ctggaaggag cgcttgaggc gccagtcgag ggggtgctcg ccctgggcgt
     4141 cgcggaactg ggccaccggg ctccccagca ggcccaaccg gtagaccggc cgcgaggcct
     4201 ccgcccgcgg cggcggctcg cgcggcgtgg gacgggacga gtcgggggcc gccttcggcg
     4261 tcccccggcg ctgcctctcc cgttccagca tcgcggcgag cagccgcggc aggcggtcgc
     4321 gctgatcccc ctcgatccag agcccccggg actcgagcag atgccaggcc gcctcgccgt
     4381 catccctctc gtcgttctct agcgagctgg ccagcagcag gtcccgctcc tccgtcgaga
     4441 aggagtgcag cacctcgtgg cggaggaagt agcgcaccgg agagacctcc aggagctgct
     4501 cgggcgtcgg ctggtcgagc ccgacgccgc cggtggcgtc cagggccagc cggagcgggc
     4561 ggtaccagcc gtcgctcgcc tcgcagagct cgcgcgcccc ggccgggtcg agctcgccgc
     4621 cggtgagcaa gtaccagagg gtcgccacct cgcgcgagtc gaggagcatc tcctgcggcg
     4681 acagcacggc gcaggggacg gaggacggcg cccacctcga ttc
//
LOCUS       DASPJI010000468         8662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_374028, whole genome shotgun sequence.
ACCESSION   DASPJI010000468 DASPJI010000000
VERSION     DASPJI010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8662)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374028"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VEL74_12735"
     CDS             complement(<1..433)
                     /locus_tag="VEL74_12735"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611016.1"
                     /note="catalyzes the release of the N-terminal amino acid
                     from a tripeptide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HYG63440.1"
                     /translation="MSYRYSCVDRFLRYVTFDTQASETSETYPSTEKQLVLLRHLAGE
                     LRALGLADVAMDEHGYVMATIPATTRKTEVPVIGFIAHVDTSPEMSGENVKPIVHRGY
                     QGQDLVLPDDPTAVLRPADSPYLRERIGDDIITASGTTLLGA"
     gene            665..1228
                     /locus_tag="VEL74_12740"
     CDS             665..1228
                     /locus_tag="VEL74_12740"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HYG63441.1"
                     /translation="MAVDPIPRKLTYEDYLLFPEEDGLRHELIDGEHFVSPAPSRKHQ
                     KASWNLAVFIGSYLRENPLGEAYTAPFDVVLSRHDVVQPDLIFVSDERLAILGDKNAQ
                     GSPDLVIEILSTGTRKVDETLKLRLYERSGAREYWMLDPEGETARVYRREGDGFSLAR
                     DASASSGDELETPLLPGLRIPLADLFR"
     gene            1429..2742
                     /gene="lpxC"
                     /locus_tag="VEL74_12745"
     CDS             1429..2742
                     /gene="lpxC"
                     /locus_tag="VEL74_12745"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HYG63442.1"
                     /translation="MSELILIVDDEPGILSTLGAILSDEGYEVLTSTSGEEAVSLYEE
                     KRPAVVFLDIWLADRDGLETLQALREADTAAAVVMMSGHGTASTAVKAIKMGAFDYLE
                     KPLSYRRAVEAAAGALAYRRSLVTGAAGALGAGEAALPASPRDRFDGERRFTAPPALP
                     LLAETGRGQRTLRQSTVVYGLGLHSGQRTGMSLHPLPENSGIHFVTLPTGVEIPAHVR
                     AVADTDYATTLSGEGENIRTVEHLLSALHAYGVSNLLIKVHGEIPVLDGSALEFCKIL
                     EEVGLAEQDEPQREVVIDRRYEVGGAGEKVLAIEPADVFSVSYLLRYPPPVGEQSYEF
                     SMTSAEDYKREIAPARTFGFMKDLKMLNELGLGSGGRLDNFILVGEDEVINTELRFPD
                     EFVRHKILDVIGDLYLAGFPIRGKVTARLTGHRDNIALLKRIVAG"
     gene            complement(2720..4279)
                     /locus_tag="VEL74_12750"
     CDS             complement(2720..4279)
                     /locus_tag="VEL74_12750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG63443.1"
                     /translation="MSSSPQPQTPDPAERVPVEELLRRSQENLQFIADATPVNIAHCS
                     ASCHFLFVNRAYAARFGLTPDQVVGRPIAEVLGQAAYEAIRPYVDRVLTGERFEFETE
                     IDYRDIGRRYMHVAYTPEIGAEGKVVSWVASVTDLTDRHRLEEALREADRRKDEFLAT
                     LAHELRNPLAPIRNAVHLARVKGPPDPNLRWAQEVIERQVQQLTRLVDDLLDVSRITR
                     GKIDLRRERVELSAVIERAMETSRPLIEAAGHRLTVIFPERRLLLDADPTRVSQVLSN
                     LLNNAAKYTSDGGHIELIAGQEGEEAVIRVRDDGIGIPKEMLGLIFEMFTQVDTSLER
                     AQGGLGIGLTIARRLVEMHGGTLEAASEGPGRGSELTVRLPLAPAETAGPDLLEDEGA
                     AKAGAARRILVVDDNLDSAESLGMLLQIMGHDVRVAHDGLRALEVAAEHRPEVVLLDI
                     GMPGMNGYDVARRLREWDATRSSLLVAMTGWGQDEDRRRSKEAGFDHHLVKPLDPKSL
                     EGLLATPPRSA"
     gene            complement(4325..5509)
                     /gene="argH"
                     /locus_tag="VEL74_12755"
     CDS             complement(4325..5509)
                     /gene="argH"
                     /locus_tag="VEL74_12755"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HYG63444.1"
                     /translation="MARLWDKGEPLDARVLRYTAGEDHRLDARLVPYDVRASIAHAEM
                     LHGAGLLPAEDLAAIREGLEALAAGHAAGEWEITLQEEDVHTALESRLTALIGEAGAR
                     LHLGRSRNDQVLAALRLSLRDEVEALRQGALKVAAALDDLAAREKETPLPGYTHLQPA
                     MPSSVPLWAGGYAAELRDDADGLAAARRRVDKNPLGSAAGYGVPLLPIDREATRASLG
                     FAEVHEPVTAVQLSRGKAEAQVLFEIALLMQDLGRLASDLVLFAMDELGFVSLPAAMT
                     TGSSIMPQKRNPDVFELVRGRTATAQACLAEVLGIPAKLPSGYHRDLQLLKAPLFRGI
                     DLARDTLAVMAEALAGVRFRPERVRLAPGLYAAEEAYRLVLEEGIPFREAYRRVGERY
                     RS"
     gene            complement(5509..6621)
                     /locus_tag="VEL74_12760"
     CDS             complement(5509..6621)
                     /locus_tag="VEL74_12760"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYG63445.1"
                     /translation="MRLPEREVLDLHRAIVGIPSVSGSEAALADYLEDYLARYLGRQG
                     AAPLRVGNSLLALHGASAGPVLLLNTHLDTVPPAPGWSRDPWTVDEEEGKVHGLGSND
                     AKASVAAMVAAYLAFVEARLPMTLALALVEGEETRGTGTEAVLAELARRGLPLEAAVV
                     GEPTGLDVAVAQKGLMVLELVARGDACHAANASALGAVNAARLLARDLVALEGLDFGP
                     PHEHLGPITLEPTQVRAGTARNVLPGEATAILDVRTTPALSREEIVRRVRAAVQGEVR
                     VLSDRLLPKSTPAGAPLVEAARRVRPEARVFGSATLSDMAFLNGTPAIKCGPGRTERS
                     HTPDEYVLASEVLDGARFYTRLIGAYLESRGQEQAA"
     gene            complement(6618..7574)
                     /gene="argB"
                     /locus_tag="VEL74_12765"
     CDS             complement(6618..7574)
                     /gene="argB"
                     /locus_tag="VEL74_12765"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HYG63446.1"
                     /translation="MKDVGQRSAEMAALKHAAPYVRLFKGKTFVLKAGGEALGTEGAI
                     RSLLEQVDILYQVGIRVVLVHGGGGESSDLLRALGGEPRFVEGRRITDEKALEVAAMV
                     LNGTVNTRVLAAGRAVGLPTVGLSGIDAGLVQARRRPPVVMGGGERVDYGFVGDVVSV
                     APGVVQTLLDAGMVPVVSPLSAADDGTVLNINADTVAAALAAGLKAEKLILLTGAPGI
                     LERPGDGSTLVSYTDLAGLEQLREQGCLIEGMLPKASAIEAAIRGGVRRVHIISHSLR
                     DGLLAEVFTNEGVGTLVVEDLNALTPAEQFAGSKPEAVGART"
     gene            complement(7571..8584)
                     /locus_tag="VEL74_12770"
     CDS             complement(7571..8584)
                     /locus_tag="VEL74_12770"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HYG63447.1"
                     /translation="MKGFVDLADLAPGEVRELLALADRLERRPEPQALAGRVLALLFF
                     NPSLRTLASFQSGMARLGGSSFVISPGQGSWQLETRLGAVMDGAAAEHVREAIPVLAG
                     YADALGIRSFAGGTDLEADLADESFHGMAGVCPVPLVNLESAINHPCQALADWKTLDE
                     LAIPDNGGKFVLSWAWHPKALPLAVPAAAVHMAALRGMDVTVLRPEGFALPKPVMERA
                     RRAAALSGGSVRETDDRTEAMTGAHVIYAKSWTAPSRYGDPQGEADLRAGLRSWCVDE
                     GWFAPAAQDCRFMHCLPVRRNVVVRDEVLDGPRSVVIREAHNRMWTQMAVLHRLLSGG
                     GAQ"
BASE COUNT         1213 a         3103 c         3111 g         1235 t
ORIGIN      
        1 cggcgccgag gagcgtcgtg cccgaggcgg tgatgatgtc gtcgccgatc cgctcgcgca
       61 ggtaggggct gtcggccggc cgcagcaccg ccgtcgggtc gtcgggcagc accaggtcct
      121 ggccctgata gccgcggtgg acgatcggct tgacgttctc gccgctcatc tcgggcgagg
      181 tgtcgacgtg ggcgatgaag ccgatgaccg gcacctcggt cttgcgggtg gtggccggga
      241 tggtggccat gacgtagccg tgctcgtcca tggcgacgtc cgcgaggccg agggcccgga
      301 gctcgccggc gaggtgacgc aggagcacga gctgcttctc ggtgctcggg taggtctccg
      361 aggtctcgga ggcctgggtg tcgaaggtga cgtagcgcag gaagcggtcg acacaggagt
      421 agcggtagct cacgggggtc tccctcctct gggggccggc cgccatcggt caggcggcgc
      481 cggcgcttgg gggcgcctgg gaaggctagc cgaggagagg aggggccgca aggggcgcgc
      541 gaaggcaccc gctccccgtc acctctcgcc gttccgggtc gccggatgtg gtaccctccg
      601 ccgtcccggg agaggaaaga atgaccgtgc tagaatgtcc ttgcaggacc gaggaggaga
      661 cgtcatggcc gtcgacccga ttccccgcaa gctcacctac gaggactacc tcttgttccc
      721 ggaggaggac ggcctccgtc acgagctgat cgacggagag cacttcgtgt cgcccgctcc
      781 ctcccggaag caccagaaag cctcgtggaa tctggcggtc ttcatcggct cctacctccg
      841 cgagaatcct ttgggggagg cctatacggc tccgttcgac gtggtcctgt cgcggcatga
      901 cgtggtccag cccgacctga tcttcgtctc ggacgagcgg ctggcgatcc tgggagacaa
      961 gaacgcccag ggctccccgg acctggtcat cgagatcctc tccacaggca cccgcaaggt
     1021 ggacgagact ctgaagctcc ggctctacga gcgctccggc gcccgggagt actggatgct
     1081 ggacccggag ggggagaccg cacgggtgta ccggcgagag ggagacggct tctccctggc
     1141 ccgggacgcc tccgcttcct cgggcgacga gttggagacc cctctcctcc cagggcttcg
     1201 catccctctc gccgacctct tccggtaggc ctacctcccg gcggcgaggc ccccaccctc
     1261 cggggtaggc cctttagaaa gctctgcccg tactcttccc ctcggagccc aggacaaatc
     1321 ggatcgaagg ttggtggaca cctctccccc ggggctccgc cctgccctgc cggagcgatc
     1381 tccggcgggg ccttgctatg ctacggaccg gaagggatgg gtcgacgcat gtccgagctg
     1441 atcttgatcg tggatgatga gccggggatc ctctccaccc tcggcgcgat tctctccgac
     1501 gaggggtacg aggtcctcac cagtaccagc ggcgaggagg ccgtgagcct ctacgaggag
     1561 aagcggcccg ccgtcgtctt cctcgacatc tggctggccg accgggacgg gctggagact
     1621 ctccaggccc tgcgcgaggc cgataccgcg gccgccgtgg tcatgatgtc ggggcacggc
     1681 acggcgtcca cggcggtcaa ggcgatcaag atgggcgcct tcgactacct ggagaagccg
     1741 ctctcctacc ggcgggccgt cgaggccgcc gccggtgcgt tggcgtaccg gcggtcattg
     1801 gtcaccgggg ccgccggcgc tctcggcgcc ggggaggccg ccctgccggc ttcgccccgc
     1861 gaccgcttcg acggcgagcg ccggttcacc gcgccgcccg cgctcccgct gctcgccgag
     1921 accggccgcg ggcagcggac gctgcgccag tcgacggtgg tctacggact cgggctccac
     1981 tccggccagc gcaccggcat gtcgctccac ccgctgccgg agaacagcgg catccatttc
     2041 gtcaccctgc cgacaggggt ggagatcccc gcccatgtgc gcgccgtggc cgacaccgac
     2101 tacgccacga ccctctccgg ggagggggag aacatccgca ccgtcgagca cctcctgtcg
     2161 gcgctgcatg cctacggggt gagcaacctg ctgatcaagg tgcatggcga gatcccggtg
     2221 ctcgacggct cggcgctgga gttctgcaag attctggagg aggtggggct ggcggagcag
     2281 gacgagccgc agcgcgaggt ggtgatcgac cgccgctacg aggtcggggg agcgggggag
     2341 aaggtgctgg ccatcgagcc cgctgacgtc ttctcggtct cctacctgct gcgctatccg
     2401 ccgccggtcg gcgagcagtc ctacgagttc tcgatgacgt cggccgagga ctacaagcgg
     2461 gagatcgccc cggcccgcac cttcggcttc atgaaggacc tcaagatgct caacgagctg
     2521 ggtctgggct cgggcggccg gctcgacaac ttcatcctgg tgggcgagga cgaggtcatc
     2581 aacaccgagc tccgtttccc cgacgagttc gtccgccaca agatcctgga cgtcatcggc
     2641 gacctctacc tggcgggctt tcccatccgc ggcaaggtca ccgcccgcct caccggccac
     2701 cgcgacaaca tcgccctgct caagcggatc gtggcggggt agcgaggagg ccctccaggc
     2761 tcttggggtc gagcggcttg accagatgat ggtcgaagcc cgcctctttc gagcgccgcc
     2821 ggtcctcgtc ctgcccccat ccggtcatgg ccaccagcag cgagctccgg gtggcgtccc
     2881 actcgcggag ccgccgggcc acgtcatagc cgttcatccc cggcatcccg atatcgagca
     2941 ggaccacctc ggggcggtgc tccgcggcta cctccagagc ccgcaggccg tcatgcgcga
     3001 cacgcacgtc atgccccatg atctggagca gcatgcccag gctctcggcg gagtccaggt
     3061 tgtcgtcgac caccaggatg cggcgggcgg cacccgcctt cgccgcgccc tcgtcctcca
     3121 ggaggtccgg cccggcggtc tccgccggcg ccagcggcag gcgcacggtg agctcgctgc
     3181 cccggccggg cccctcgctc gcggcctcga gcgtgccccc gtgcatctcc accaggcgcc
     3241 gggcgatggt gaggccgatc cccagcccac cctgggcgcg ctccagcgag gtgtccacct
     3301 gggtgaacat ctcgaagatc aggccgagca tctccttcgg gatgccgatg ccgtcgtcgc
     3361 ggacccggat cacggcttct tctccctcct ggccggcgat cagctcgatg tggccaccgt
     3421 cactggtgta cttggccgca ttgttgagga ggttggagag gacctgggag acgcgggtgg
     3481 ggtccgcgtc caggagcagc cggcgctcgg ggaagatgac ggtgagccgg tggccggcag
     3541 cttcgatcag cgggcggctg gtctccatcg cccgttcgat gaccgccgac agctccaccc
     3601 gctcgcggcg caggtcgatc ttgccgcggg tgatgcgcga gacgtccagc agatcgtcca
     3661 ccagccgggt gagctgctgg acctgccgct cgatgacctc ctgcgcccat cgcaggttgg
     3721 ggtccggcgg ccccttcacg cgggccaggt ggacggcgtt gcggatcggt gccagggggt
     3781 tgcgcagctc gtgcgccagc gtcgccagga actcgtcctt gcgacggtcg gcctcgcgca
     3841 gcgcctcctc caggcggtgg cggtccgtga ggtcggtgac ggaagccacc caactcacca
     3901 cctttccctc ggcgccgatc tcgggggtgt aggcgacgtg catgtagcgc cggccgatgt
     3961 cccggtagtc gatctcggtc tcgaactcga agcgctcacc ggtgaggacg cgatcgacgt
     4021 agggccgtat cgcctcgtag gccgcctgtc ccagtacctc ggcgatgggg cggcccacca
     4081 cctgatcggg agtcaggccg aagcgggcgg cataggcccg attgacgaac aggaagtggc
     4141 agctggcgct gcagtgcgcg atgttgaccg gcgtcgcgtc ggcgatgaac tgcaggttct
     4201 cctggctacg gcgcagcagc tcttccacgg gcacgcgctc ggcagggtcg ggagtctggg
     4261 gctgcggaga ggaggacacg ggtaggctca tcgcaattgt atgccgcgcc gggtccggcc
     4321 ggcttcagct ccggtagcgt tcccccacgc ggcgataagc ctcgcggaag gggatcccct
     4381 cctccagcac cagccggtag gcctcctcgg cggcgtagag gccgggggcg agacggacgc
     4441 gctcgggccg gaagcggacg ccggccagcg cctcggccat caccgccagg gtgtcgcggg
     4501 cgaggtcgat cccccggaag agcggggcct tgaggagctg gaggtcgcgg tggtagcccg
     4561 aagggagctt ggcggggatg cccagcacct cggcgaggca ggcctgggcg gtcgccgtcc
     4621 ggccgcgcac cagctcgaag acgtcggggt tccgtttctg cggcatgatc gacgagccgg
     4681 tggtcatcgc cgccggcagg ctgacgaatc cgagctcgtc catggcgaag agaaccaggt
     4741 cggaggcgag cctccccagg tcctgcatca gcagggcgat ctcgaacagc acctgggctt
     4801 cggccttgcc gcgcgagagc tggacggcgg tcaccggctc gtggacctcg gcaaagccca
     4861 gcgaggcccg cgtggcctcg cggtcgatcg ggagcagggg gacgccgtag cccgccgccg
     4921 agcccagcgg gttcttgtcg acgcgccggc gggccgcggc gagtccgtcg gcgtcgtcgc
     4981 gcagctcggc ggcgtagccg ccggcccaca aagggaccga gctgggcatc gcgggctgca
     5041 ggtgggtgta gccggggagc ggcgtctcct tctcccgcgc ggcgaggtcg tccagcgccg
     5101 ccgccacctt cagcgccccc tgccgcaggg cctccacctc gtcgcgcaga gagagccgta
     5161 gcgccgccag cacctggtcg ttgcgcgacc gccccaggtg gaggcgggcg ccggcctctc
     5221 cgatcagcgc ggtcagccgc gactcgaggg cggtgtggac gtcctcctcc tggagcgtga
     5281 tctcccactc gccggccgcg tgcccggcgg ccagggcctc caggccctcg cggatggcgg
     5341 ccaggtcttc ggcggggagc aggccggcgc catggagcat ctcggcgtgg gcgatggagg
     5401 cgcgcacgtc gtaggggacg agccgggcgt ccagccggtg gtcctcgccg gcggtgtagc
     5461 ggagcacccg agcgtcgagc ggctccccct tgtcccacag acgggccatc aggccgcctg
     5521 ctcctgcccc cggctctcca ggtaggcgcc gatgagccgg gtgtagaagc gggcgccgtc
     5581 cagcacttcg ctcgccagca cgtactcgtc cggcgtgtga gagcgctcgg tccggcccgg
     5641 cccgcacttg atggccggcg tgccgttgag gaacgccatg tccgacaggg tcgccgagcc
     5701 gaagacccgc gcctcggggc ggacgcggcg ggcggcctcg accagcggcg ctccggccgg
     5761 cgtcgacttg gggaggaggc ggtcggagag cacccgcacc tccccctgca cggcggcgcg
     5821 tacccggcgc acgatctcct cccgggagag ggcgggcgtg gtgcggacgt ccaggatcgc
     5881 cgtcgcctca ccagggagca cgttgcgcgc cgtgccggcc cgcacctgcg tcggctccag
     5941 ggtgatcggg ccgaggtgct cgtggggggg gccgaagtcg agcccctcca gggcgaccag
     6001 gtcgcgggcg aggagccggg cggcgttgac ggcgccgagg gcggaggcgt tggcggcgtg
     6061 gcaggcgtcg ccgcgggcca ccagctccag aaccatcagc cccttctggg ccacggcgac
     6121 gtcgaggccg gtgggctcgc cgaccaccgc ggcctccagc ggcagcccgc ggcgggcgag
     6181 ctcggcgagc accgcctcgg tgccggtgcc ccgcgtctcc tccccctcga ccagggcgag
     6241 cgccagcgtc atgggcagcc gcgcctcgac gaaggcgagg taggccgcga ccatggccgc
     6301 caccgacgcc ttggcgtcat tcgagcccag gccgtggacc ttgccctcct cctcatccac
     6361 cgtccacggg tcgcggctcc agcccggggc cggcggcacg gtgtccaggt gggtattgag
     6421 cagcagcacc ggaccggcgc tcgccccgtg cagggcgagg aggctgttgc cgacgcgtag
     6481 cggcgcggcc ccctgccgtc cgagataacg ggcgaggtag tcttccagat agtcggccag
     6541 cgccgcctcg ctgccggaga ccgacgggat gccgacgatg gcgcggtgga ggtcgagcac
     6601 ctcccgctcc ggcagcctca cgtgcgcgct cccacggctt cgggcttgct gccggcaaac
     6661 tgctcggccg gggtcagggc gttcagatcc tcgaccacca gcgtccccac cccctcgttg
     6721 gtgaacacct cggccagcag gccgtcgcgc agcgaatggg agatgatgtg gacccgccgc
     6781 acgccgccgc ggatcgccgc ctcgatcgcg ctcgccttgg ggagcatccc ctcgatcagg
     6841 cacccctgct cgcggagctg ctcgagcccg gccaggtcgg tgtaggagac cagggtcgag
     6901 ccgtcccccg gccgctccag gatgccgggc gcgccggtga gaaggatcag cttctcggcc
     6961 ttgagccccg ccgccagcgc cgcggccacg gtgtcggcgt tgatgttgag caccgtgccg
     7021 tcgtcggcgg cggagagggg gctcaccacc ggcaccatgc cggcgtcgag cagggtctgg
     7081 accacccccg gcgcgacgct caccacgtcg ccgacgaagc cgtagtccac ccgctcgccg
     7141 cctcccatca cgaccggtgg ccggcggcgc gcctggacga gcccggcgtc gatccccgac
     7201 aggccgaccg tcggcaggcc cacggcacgg ccggcggcga ggacgcgggt gttgaccgtg
     7261 ccgttcagca ccatggccgc cacctccagc gccttctcgt cggtgatccg ccgcccctcg
     7321 acgaagcgcg gctcgccgcc cagggcgcgc agcaggtcgc tcgactcgcc gccgccgccg
     7381 tgcaccagga cgacgcggat ccccacctga tagaggatgt ccacctgctc gagcagcgag
     7441 cggatggctc cctccgtgcc gagggcctcg cccccggcct tgaggacgaa ggtcttgccc
     7501 ttgaacagcc ggacataggg cgccgcgtgc ttcaaggccg ccatttcggc ggaccgctgg
     7561 ccgacgtctt tcattgggca cctcctcccg agagcaggcg gtggaggacc gccatctggg
     7621 tccacatgcg gttgtgcgcc tcgcggatca cgacgctccg cgggccgtcc agcacctcgt
     7681 cgcggaccac cacgttgcgg cgcaccggca ggcagtgcat gaaccggcag tcctgcgccg
     7741 ccggggcgaa ccacccctcg tcgacgcacc aggagcggag cccggcgcgc aggtcggcct
     7801 ccccctgggg atcgccgtag cggctcggcg ccgtccacga cttcgcatag atgacgtggg
     7861 cgccggtcat ggcctcggtc cggtcgtcgg tctcgcgcac cgagccgcca gacaacgccg
     7921 cggcgcggcg cgccctctcc atcaccggct tcggcagggc gaacccctcg ggccgcagca
     7981 cggtgacgtc catcccccgc agcgccgcca tgtgcacggc cgcggcgggc acggccaggg
     8041 ggagcgcctt ggggtgccag gcccaggaga ggacgaactt gccgccgttg tccggaatgg
     8101 cgagctcgtc cagggtcttc cagtccgcca gcgcctggca ggggtggttg atcgccgact
     8161 ccagattgac cagcggcacc gggcagaccc cggccatgcc gtggaaggac tcgtcggcca
     8221 agtccgcttc gaggtcggtg ccgccggcga acgagcggat gcccagcgcg tcggcgtagc
     8281 cggcgaggac cgggatcgcc tcccggacgt gctccgccgc ggcgccgtcc atcaccgcgc
     8341 cgagccgcgt ctcgagctgc cagctcccct ggcccggcga gatgacgaac gacgagccgc
     8401 ccaggcgcgc catgcccgac tggaacgagg ccagggtgcg cagcgacggg ttgaagaaga
     8461 ggagcgccag caccctgccg gccagagcct ggggctccgg ccgccgctcc aggcggtccg
     8521 ccagcgccag cagctcccgc acctcgcccg gagcgagatc cgccaggtcg acgaacccct
     8581 tcacgggcgg atctccgaca gggcgcgggc gagggcgtcc acgtgggagg cctcgaggac
     8641 cagcggcggc agcagccgca cg
//
LOCUS       DASPJI010000469         9579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_87466, whole genome shotgun sequence.
ACCESSION   DASPJI010000469 DASPJI010000000
VERSION     DASPJI010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9579)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9579
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87466"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /gene="modA"
                     /locus_tag="VEL74_12775"
     CDS             <1..627
                     /gene="modA"
                     /locus_tag="VEL74_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HYG63448.1"
                     /translation="EEEGGDRIVLNLGASSTLARQIEEGAPADLFLSADEEKMNRLEK
                     RKLLLPGTRRSVLSNTLVIVVPEDSRLKITGARDLAAPAVRVLALAEPQTVPAGIYAK
                     EYLGRLGVWSRIADRVVPTENVRAALAAVEAGNADAGIVYKTDARTAKRVRVAYEVPR
                     EKGPAISYPFAVLAESARQAAARKFLAYLESAKALTVFRKHGFLILSD"
     gene            complement(674..1093)
                     /locus_tag="VEL74_12780"
     CDS             complement(674..1093)
                     /locus_tag="VEL74_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63449.1"
                     /translation="MSEKTKESSGLYLPLNHLEDSTEAVQSDVGAGTIVYHDASGPLP
                     LASQTFESPAGTLIITCSGSGSAPKAPDTIGMGISIDNRFAGVAYVRTLGTETKFFAN
                     TFAFGPVTAGTHTMYAFPLGNTVTSADDVFNVSFTAQ"
     gene            complement(1223..1405)
                     /locus_tag="VEL74_12785"
     CDS             complement(1223..1405)
                     /locus_tag="VEL74_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63450.1"
                     /translation="MKRTLRGKMTLSKETIRLLGEPEELVALRHVAGAATGDTTPCPK
                     YTFCGVVSGETVRFCS"
     gene            1575..2276
                     /locus_tag="VEL74_12790"
     CDS             1575..2276
                     /locus_tag="VEL74_12790"
                     /inference="COORDINATES: protein motif:HMM:NF021141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HYG63451.1"
                     /translation="MTRWLLRGLGLLLLLAILPSLYACVSSAPPPAVTLPGPPPASAS
                     PTVPLYVADWGYHSAIIVPQPAGWELGPPGEETAPFLEYAWGDRRFYMENNFWPHSVF
                     ATLFLPTRTVTYVDGWPGAPAMDDKPRALYTRGITVEQWATLLQELERWIPRDAAGVR
                     PPAFPAVKGYEGRFYPGYGTYFWWMDCNRWTVERLQAAGLAGSGRGVVFSGQIAPRLR
                     GFTRVTPLGDPAPIR"
     regulatory      2287..2371
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            2432..3874
                     /gene="ahcY"
                     /locus_tag="VEL74_12795"
     CDS             2432..3874
                     /gene="ahcY"
                     /locus_tag="VEL74_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HYG63452.1"
                     /translation="MTVAMSVETSVEEKSAGTQTDYHVADISLAEWGRKELLIAEGEM
                     PALMAIRQELAASQPLEGARIAGSLHMTIQTAVLIETLQALGAEVRWASCNIYSTQDH
                     AAAAIAAVGTPVFAYKGESLAEYWDFTHRIFEFPEGTANMILDDGGDATLLVHLGAKA
                     EDDPSVLDNPTSEEERVLFAAIRRRNASQPGWYKKQLAAIQGVTEETTTGVHRLYEMA
                     KRGDLRFPAINVNDSVTKSKFDNLYGCRESLVDAIKRATDVMVAGKIAVVCGYGDVGK
                     GSAQALRALSAQVWITEIDPICALQAAMEGYRVVTMDEACEVADIFVTATGNFHVITH
                     DHMRRMKHNAIVCNIGHFDSEIDVASLEQYPWEEIKPQVDHVIFPEGNRIILLAKGRL
                     VNLGCGTGHPSYVMSSSFANQTLAQVELWTKKGEYKVGVYTLPKQLDEKVARLQLTTL
                     NAKLTELRDDQAKYIGVPKEGPYKTDWYRY"
     gene            complement(3951..6245)
                     /locus_tag="VEL74_12800"
     CDS             complement(3951..6245)
                     /locus_tag="VEL74_12800"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HYG63453.1"
                     /translation="MMVSRPSRWAGARVAALALALTLVMAAGALAKPRQNDAAPVDPE
                     APLRQFEVSPAKSEIRIDGTLDEAAWQSATVVDLPYEWFPGDNVTPKAKTDSYITFDS
                     EHLYIAFRAYDPAPSAIRAHLMDRDVITPFVQDDHVGFSLDTFNDERRAYQFRINPLG
                     VQVDAVFNEMDGLEDFSWDAIWRSAGRVDEQGYTIEVAIPFKQLRFPRTTGPQTWGFE
                     AFRNYPRNVRHRMSSRYTDRAKQCLLCQENKLSGFQGIAPGRNLEITPTVTAQRDDQL
                     TDSSPDALQPGESEFEPGITARWGITPNVSLNAAVNPDFSQVEADAAQLSINTRFSLY
                     YPEKRPFFLEGADLYTTPLEAVFTRTVAEPTWGAKLNAKEGKNGVGVFMAQDDLNNLI
                     LPSNQGSSLVSLDEQVTSGVVRYRRDIGARSVLGVLYTGREGDTYHNRVGGLDGVFRL
                     TKSATVRFQYLMSDTQYPGALAEEYEQVPDAFTGDAFKVQYDHFSDNWRGYVRYTDLD
                     PAFRADSGFVPRVDVRTAEAQYERFLHGSSDKWYSQMTFGTRGLRTDDHDGRQTDENV
                     EVFGTYWGPKQSYLEVDVAQKQELYGPELYDLEQQRIYFRISPNGKTRLGFDGRFGDE
                     IDYDNARLGKITMWNPLVQLRVGRRTNLQLNHLMQKLEVDQGTVFDAGLSQMQMVYQF
                     NVRTFVRAIVQYTDIDRDSALYRETVDPSEEDVFGQFLFSYKLNPQTVFFVGYTDSRE
                     ALNERDLTQTGRTFFMKLGYALLY"
     gene            complement(6311..7681)
                     /locus_tag="VEL74_12805"
     CDS             complement(6311..7681)
                     /locus_tag="VEL74_12805"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYG63454.1"
                     /translation="MSDPKTGGMKTFGIIWIGQLVSLLGSGLTSFALGIWVLRHTDSV
                     TQYTLTILLASLPGVLISPLAGALVDRWDRRWVMFWSDVGPGVVTLVYSYLLWQDSLT
                     LWHVYVGVAINALFSAFQWPAYIAAITMLVERKDYGRVNGMLEFGQAGTSIAAPALAA
                     LFLTFMDMEGILLLDFVTFLFAAGTLLLVRIPKPEASAEGEAGKGSLWKEVAFGWHYI
                     RQRPGLFHLLMFFAMVNLVASLCGVALIPMVYGFASEAGVGTIMSLVGIGMLLGGFIM
                     TTTGGPKPRVYGVLGGGLLFSVCFILVAIKPSLPLVGLGVILWYTGIPIMNASSSAIW
                     QAKVAPDVQGRVFSMRRMIAQFTIPIGDFGAGPLADLVFNPLMMAGGALAGSAGRVIG
                     TGPGRGIALMFLSMAFIPALAAVWGLLNPRLRNVEAELPDAEKKVSSNSPDAAPEEQE
                     TAAQAS"
     gene            complement(7694..>9579)
                     /locus_tag="VEL74_12810"
     CDS             complement(7694..>9579)
                     /locus_tag="VEL74_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG63455.1"
                     /translation="PGIAQCVVLAREDTPGSKRLVAYLVPLAGAGVNGNGHAHHAPAG
                     PKAAELKEFLKERLPEYMVPALFVTLDALPLTPNGKIDRRTLPAPDQGRAEERPYVAP
                     TNPVEETLAALWAEILDVPKVGIHDDFFDLGGDSLTVIRVVAKANKGGLGITTKQLFQ
                     NRTVADLARVAGTTHILAEQGLVTGPVPFTPAQLHFLELEHANPRFHSLGALLEIKEP
                     PMDLAALEKALRAVLSQHDNLRVRLVTDEGGPHLVTDAPWKDLDLLRIDVSGLPEESR
                     EDTFRGAASLARTFDLTQGPLFRAVVFDHGHVQFPFLAAHFFTADVGSWQTLLDDLDI
                     AYRQARSGRPIRLQKKTTSARQWADRLAERAHSVDMQQEREYWLSDSRRRAPRILLDM
                     PEGANLMSSSAHVRVELNEEETHALLHDVQRAYGVQIDGLLLTSVLQAFEPWTGGRSL
                     LIDLLGHGREALYDDVDLTRTAGWFNTIYPACLELPDTGDPAAEVRAVNEQLRQIPNG
                     GIGYGILRYLSRDRDFLARIKSMPQPDIFFNYFGDDHSKELVSMKKLEGFAGYALDRE
                     TRRLRQLAIGVYVQQDRMLIKWEYSRNVFRPDTIETVAQRSRERLRWYIENHSSQQGL
                     GRI"
BASE COUNT         1566 a         3125 c         3367 g         1521 t
ORIGIN      
        1 gaggaggagg gaggggaccg gatcgtcctc aacctcgggg cctcgagcac actggcgcgc
       61 cagatcgagg aaggggcgcc ggcggatctt ttcctgtcgg ccgacgagga gaagatgaac
      121 cggctggaga agaggaagct cctgctcccg gggacgcgcc ggagcgtgct gtcgaacacc
      181 ctggtgatcg tggtgcccga ggattcgcgc ctgaagatca cgggagcccg ggacctcgcg
      241 gcgcccgccg tccgcgtcct ggccctggcg gagccccaga cggttccggc cggaatctac
      301 gccaaggagt acctcggccg gctcggggtc tggagcagga tcgcggaccg ggtggtgccg
      361 acggagaacg tccgcgccgc cctggccgcg gtggaagcgg gcaacgccga tgcggggatc
      421 gtctacaaga cggacgcgcg gaccgcgaag cgggtgcgcg tcgcctacga ggtgccgcgc
      481 gagaagggcc cggcgatctc gtatccgttc gccgtgctgg ccgaatcggc gcggcaggcg
      541 gcggcccgga agttcctggc ctacctggaa tcggccaagg cgctcacggt cttccggaag
      601 cacggcttcc tgatcctgag cgattgaggc cagccaagcc ccgcgccgga gcctggctgg
      661 ccgccggagg tctctactgg gcggtgaagc tgacgttgaa gacgtcgtcc gccgaggtca
      721 ccgtgttgcc gagcgggaag gcgtacatcg tgtgggtgcc tgccgtgacc ggaccgaagg
      781 cgaaggtatt ggcgaagaac ttggtctccg tgcccaaggt ccggacgtag gccacgcccg
      841 cgaaccggtt gtcgatggaa atgcccatgc cgatggtgtc cggtgccttc ggggcgctgc
      901 ccgagcccga gcaggtgatg atcagggtcc cggccgggct ctcgaacgtc tggctggcca
      961 ggggcagggg acccgaagcg tcgtgataga cgatcgtccc cgcgccgacg tccgactgga
     1021 cggcctcggt gctgtcctcg agatggttca ggggaaggta cagcccggag ctttccttgg
     1081 ttttctcgct catatccgtc tccttggtgg aggttgtggg cggacgaaaa gctcctcggc
     1141 aggccggcta gggtcatagg acccgccgtc ccctggggac ggcgggcatc tctactggga
     1201 gcccgggcat tatagctttg ttctacgaac aaaacctcac ggtctcacca ctcaccaccc
     1261 cgcagaaggt gtacttcggg cagggagtcg tgtcgccggt cgcggcgccg gcgacatggc
     1321 gcagcgccac caactcctcg ggctccccca aaagcctgat ggtctctttg ctcaaggtca
     1381 tcttgccacg gagcgttctt ttcatgtttg cggtctcctc gatggatgcg gtgaagggct
     1441 gcctccccat ccgctttcct ggccagcccc taccttgcag gtgcgagacg ccggtgccct
     1501 cgcgcctcgt cgcagaccgg gatagactcg cctcgatgcg cctccgccgc cgagggagca
     1561 accgaagacg cccggtgacc cgctggctgc tgcgcgggct gggattgctc ctcctgctcg
     1621 ccatcctgcc gtcgctctac gcgtgcgtca gctccgctcc gccaccggcc gtcaccctgc
     1681 ccggcccgcc ccccgcgagc gcctccccga ccgtgccgct ctacgtggcc gactggggct
     1741 accactcggc gatcatcgtg ccgcagccgg cgggctggga gctcgggccg cccggggagg
     1801 agacggcgcc gttcctggag tacgcctggg gggaccgccg cttctacatg gagaacaact
     1861 tctggcccca ttcggtgttt gccacgctgt tccttcctac ccggacggtc acctatgtgg
     1921 acggctggcc gggggcgccg gcgatggacg acaagccgcg ggcgctgtac acgcgcggca
     1981 tcaccgtcga gcagtgggcc accctgctcc aggagctcga gcgctggatc ccccgcgacg
     2041 cggccggcgt gcggcccccc gccttcccgg cggtcaaggg gtacgagggg cggttctacc
     2101 cgggctacgg gacctacttc tggtggatgg actgcaaccg ctggaccgtg gagcggctcc
     2161 aggccgccgg cctcgccggc tccggccgcg gagtggtctt ctcggggcag atcgcccccc
     2221 ggctgcgcgg attcacccgg gtcacccctc ttggcgatcc ggccccgatc cggtaaaata
     2281 cgggttccgt ccgaggagcg ttgcaggagg tcccccctcc caggctcgga cgccggcatg
     2341 accggagcac aggaaacggc gctcacctca accctttccg ggtcaggagg tgaggcctcc
     2401 ctgtcacccg gaacctccga ggaggtcaat catgaccgtc gccatgtccg tagaaacatc
     2461 cgtagaagag aagtccgcag gcacccagac cgattaccac gtcgccgaca tctccctcgc
     2521 cgagtggggc cgcaaggagc tgctgatcgc cgagggcgag atgcccgccc tgatggccat
     2581 ccgccaggag ctcgcggcga gccagccgct cgagggcgcg cgtatcgccg gctccctgca
     2641 catgaccatc cagacggcgg tgctgatcga gaccctgcag gccctcggcg ccgaggtgcg
     2701 ctgggcgagc tgcaacatct actcgaccca ggaccacgcc gccgcggcca tcgccgccgt
     2761 cggcaccccg gtcttcgcct acaagggcga gagcctggcg gagtactggg acttcacgca
     2821 ccgcatcttc gagttccccg aaggtacggc caacatgatc ctggacgatg gtggcgacgc
     2881 caccctgctc gtccacctgg gcgccaaggc cgaggacgac ccgtcggtgc tggacaaccc
     2941 gaccagcgag gaggagcggg tgctcttcgc cgccatccgg cgccgcaacg ccagccagcc
     3001 cggctggtac aagaagcagc tcgcggcgat ccagggggtc accgaggaga cgaccacggg
     3061 cgtccatcgc ctgtatgaga tggcgaagcg gggcgacctg cggttccccg ccatcaacgt
     3121 caacgacagc gtcaccaaga gcaagttcga caacctctac ggctgccgcg agtcgctggt
     3181 cgacgccatc aagcgcgcca cggacgtgat ggtggccggc aagatcgccg tcgtctgcgg
     3241 ctacggcgac gtgggcaagg ggagcgccca ggccctgcgc gcgctgagcg cccaggtgtg
     3301 gatcaccgag atcgacccca tctgcgcgct gcaggcggcg atggagggct accgcgtcgt
     3361 caccatggac gaggcctgcg aggtggcgga catcttcgtc accgccacgg gtaacttcca
     3421 tgtgatcact cacgaccaca tgcggcggat gaagcacaac gcgatcgtct gcaacatcgg
     3481 ccacttcgac agcgagatcg acgtcgcgag cctcgagcag tatccgtggg aggagatcaa
     3541 gccgcaggtg gatcacgtga tcttccccga gggcaaccgc atcatcctcc tcgccaaggg
     3601 gcggctggtg aacctcggct gcggcaccgg ccacccgagc tacgtgatga gctcctcctt
     3661 cgccaaccag acgctcgccc aggtcgagct gtggacgaag aagggcgagt acaaggtggg
     3721 cgtctacacg ctgccgaagc agctcgacga gaaggtggcg cgcctccagc tcaccaccct
     3781 gaacgccaag ctcaccgagc tgcgcgacga tcaggcgaag tacatcggcg tgcccaagga
     3841 aggcccctac aagaccgact ggtaccggta ttgataccgg cgccagccgc ctgaagcacg
     3901 aggggaccga gcgatcggtc ccccttctct tttctattgg aaagccggga tcagtacagc
     3961 agcgcgtagc cgagcttcat gaagaacgtg cgcccggtct gcgtgaggtc ccgctcgttg
     4021 agggcttcgc ggctgtcggt gtagccgacg aagaacaccg tctgcgggtt cagcttgtag
     4081 gagaagagga actggccgaa gacgtcctcc tccgacgggt cgacggtctc gcggtagagg
     4141 gcggagtcgc gatcgatgtc ggtgtactgc acgatggccc gcacgaaggt ccggacgttg
     4201 aactgataga ccatctgcat ctgcgacagg cccgcgtcga acaccgtgcc ctggtccacc
     4261 tcgagcttct gcatcaggtg gttgagctgg agattggtcc gccggccgac gcggagctgc
     4321 accagcgggt tccacatggt gatcttgccg aggcgggcgt tgtcgtagtc gatctcgtcg
     4381 ccgaagcggc cgtcgaagcc gaggcgtgtc ttgccgttgg gcgagatgcg gaagtagatc
     4441 cgctgctgct ccagatcgta cagctccggg ccgtagagct cctgcttctg cgccacgtcg
     4501 acttccaggt aggactgctt cggaccccaa taggtgccga agacctccac gttttcgtcg
     4561 gtctgccggc cgtcatggtc gtcggtgcgc aggccgcggg tgccgaacgt catctgggag
     4621 taccacttgt ccgacgagcc gtggaggaag cgctcgtact gggcctcggc ggtccgcacg
     4681 tccacccgcg gcacgaagcc gctgtcggcg cggaaggcgg gatcgaggtc ggtgtagcgg
     4741 acgtatcccc gccagttgtc cgagaaatgg tcgtactgca ccttgaaggc gtcgccggtg
     4801 aaggcgtccg ggacctgctc gtactcttcg gccagcgcac ccgggtactg cgtgtcggac
     4861 atcaggtact ggaagcgcac ggtggccgac ttggtgagcc ggaagacgcc gtcgaggccg
     4921 cccacccggt tgtgataggt gtcgccctcg cggccggtgt agagcacgcc caggaccgag
     4981 cgcgcgccga tgtcccggcg atagcgcacg acgccgctgg tcacctgctc gtccagggag
     5041 accaggctcg atccctggtt cgacggcagg atcaggttgt tgaggtcgtc ctgcgccatg
     5101 aacacgccga cgccgttctt cccctccttg gcgttgagct tcgcccccca ggtcggctcg
     5161 gcgacggtgc gggtgaagac cgcctcgaga ggcgtggtgt agaggtcggc cccctcgagg
     5221 aagaacggcc gcttctccgg gtagtaaaga gaaaagcggg tgttgatcga gagctgcgcc
     5281 gcgtccgcct cgacctgcga gaagtcgggg ttgacggcgg cgttcaggct gacgttgggg
     5341 gtgatgcccc agcgcgcggt gatgcccggc tcgaactccg attcccccgg ctgcagggcg
     5401 tcgggcgagg aatcggtcaa ttgatcatcc cgctgcgcgg tcaccgtcgg cgtgatctcc
     5461 aggttgcggc ccggggcgat gccctggaag ccgctgagct tgttctcctg gcagagcagg
     5521 cactgcttgg cgcggtcggt gtagcgcgag gacatccggt gccggacgtt cctcggatag
     5581 ttgcggaacg cctcgaagcc ccaggtctgc gggccggtgg tgcgcgggaa gcggagctgc
     5641 ttgaacggga tcgccacctc gatggtgtag ccctgctcgt cgacgcggcc ggcggagcgc
     5701 cagatggcgt cccaggagaa gtcctccagg ccgtccatct cgttgaacac cgcgtccacc
     5761 tgtacgccga gcgggttgat gcggaactgg taggcccgcc gctcgtcgtt gaaggtgtcc
     5821 aggctgaagc cgacgtggtc gtcctggacg aacggagtga tgacgtcgcg gtccatcaga
     5881 tgggcgcgga tcgccgacgg cgccgggtca taggcccgga acgcgatata gaggtgctcg
     5941 gaatcgaagg tgatgtagga gtcggtcttc gccttgggcg tgacgttgtc cccggggaac
     6001 cattcgtagg ggaggtcgac gacggtggcg gactgccacg ccgcctcgtc gagcgtgccg
     6061 tcgatgcgga tctcggactt ggccggggag acttcgaact ggcggagcgg cgcctccgga
     6121 tcgaccggcg ccgcgtcgtt ctgccggggc ttggcgagcg cgccggccgc catgaccagc
     6181 gtcagcgcca gggccagcgc ggccacacgg gcgccggccc acctgctggg acgggagacc
     6241 atcatgaaca ccctcctcca atcaaaagaa aaaactacaa aacaaccggt gctgctgaac
     6301 gttgccgcgg tcaggatgcc tgcgcagcgg tttcctgctc ttcaggtgca gcgtcgggcg
     6361 aatttgagga cacctttttt tcagcatccg gcagttcggc ctcgacattg cgcagccgcg
     6421 ggttgaggag accccatacg gcggcgaggg cggggatgaa agccatggag aggaacatca
     6481 gggcgatccc ccgtcccggc ccggtgccga tgacccgccc ggcgctgccg gcgagggcgc
     6541 cgcccgccat catcaacggg ttgaacacca ggtcggcgag cgggccggcg ccgaagtcgc
     6601 cgatggggat ggtgaactgg gcgatcatcc ggcgcatgga gaagacccgc ccctggacgt
     6661 cgggggcgac cttggcctgc cagatcgccg agctgctggc gttcatgatc gggatgccgg
     6721 tgtaccagag gatgacgccc agccccacca gcgggagcga cggcttgatc gccaccagga
     6781 tgaagcagac gctgaacagg aggccgccgc cgaggacgcc gtagacccgc ggcttggggc
     6841 cgcccgtggt ggtcatgatg aagccgccca gcagcatgcc gatgcccacc agggacatga
     6901 tcgtgccgac cccggcctcg ctggcgaagc cgtagaccat ggggatgagc gccacgccgc
     6961 agaggctcgc caccaggttg accatggcga agaacatcag caggtggaac aggcccggcc
     7021 gctggcggat gtagtgccag ccgaaggcca cctccttcca cagggagcct ttgcccgcct
     7081 ccccctcggc gctggcctcg ggcttgggga tgcgcaccag gagcagcgtg ccggcggcga
     7141 acaggaaggt gacgaagtcg aggagcagga taccttccat gtccatgaag gtgaggaaca
     7201 gggcggcgag ggcgggcgcc gcgatgctgg tgcccgcctg cccgaactcc agcatgccgt
     7261 tgacccggcc gtagtccttg cgctccacca gcatggtgat ggcggcgatg taggcgggcc
     7321 actggaacgc gctgaagagg gcgttgatcg ccacgcccac gtagacgtgc cagagggtga
     7381 ggctgtcctg ccagaggagg taggagtaga ccagggtgac gacgccgggg ccgacgtcgc
     7441 tccagaacat cacccagcgg cggtcccagc ggtccaccag cgcgccggcg agcggcgaga
     7501 tgaggacgcc cggcaggctc gccaggagga tggtgagggt gtactgggtc accgagtcgg
     7561 tgtgacggag cacccagatg ccgagggcga agctggtgag cccggatccg agcagcgaca
     7621 cgagctgccc gatccagatg atgccgaacg tcttcatgcc gccagtcttg gggtcggaca
     7681 tggatgtctc ctcctagatc ctgcccagac cctgctggga cgaatggttc tcgatatacc
     7741 agcgcagcct ctcccgggac cgctgcgcca ccgtctcgat ggtgtcgggc cggaagacgt
     7801 tgcggctgta ctcccacttg atgagcatcc ggtcctgctg cacgtagacg ccgatggcga
     7861 gctggcgcag gcggcgcgtc tcccggtcga gggcatagcc ggcgaagccc tccagcttct
     7921 tcatcgacac cagctccttg gagtggtcgt cgccgaaata gttgaagaag atgtcgggct
     7981 gcggcatgga tttgatccgt gccaggaagt cccggtcgcg gctgagatag cgcaggatgc
     8041 cgtagccgat gccgccgttg gggatctgcc ggagctgctc gttgaccgcc cgcacctcgg
     8101 ccgcgggatc gccggtgtcc ggcagctcga ggcaggccgg gtagatggtg ttgaaccagc
     8161 ccgcggtgcg cgtcaggtct acgtcgtcgt agagtgcctc gcggccgtgg cccaggaggt
     8221 cgatgaggag cgaccggccg ccggtccacg gctcgaacgc ctggagcacg gaggtgagga
     8281 gcaggccgtc gatctggacg ccgtaggcgc gctggacgtc gtgaagcagc gcgtgagtct
     8341 cctcctcgtt gagctcgacg cgcacgtgcg ccgacgagct catcaggttc gccccctccg
     8401 gcatgtcgag gagaatacga ggcgcccgcc gccgggagtc gctcagccag tactcccgct
     8461 cctgctgcat gtcgaccgag tgggcccgct cggccagccg gtcggcccac tgccgagccg
     8521 aggtggtctt cttctggaga cggatggggc gtccggaccg ggcctgccgg taggcgatgt
     8581 ccaggtcgtc caggagggtc tgccacgagc cgacgtcggc ggtgaagaaa tgggcggcca
     8641 ggaacgggaa ctgaacgtgg ccatgatcga acaccaccgc ccggaagagc gggccctggg
     8701 tcaggtcgaa ggtccgggcc aggctcgcgg caccgcggaa ggtgtcctct cggctctcct
     8761 ccggcagacc ggacacgtcg atccgcagga ggtcgagatc cttccatggc gcgtcggtca
     8821 ccagatgggg tccgccctcg tcggtcacca gccgcacccg caggttgtcg tgttgggaga
     8881 ggacggcccg cagcgccttc tccagcgccg ccaggtccat gggcggctcc ttgatctcga
     8941 gcagcgcgcc gaggctgtgg aagcgcgggt tggcgtgctc cagctcgagg aagtggagct
     9001 gcgccggggt gaaggggacc gggccggtga ccagcccctg ctccgccagg atgtgggtgg
     9061 tgccggcgac ccgcgccagg tcggccaccg tgcggttctg gaagagctgc ttggtggtga
     9121 tccccagccc ccccttgttg gccttggcga cgacgcggat gaccgtgagg gagtcgccgc
     9181 ccaggtcgaa gaagtcgtcg tggatgccca ccttggggac gtccaggatc tctgcccaga
     9241 gcgccgccag ggtctcctcc accgggttgg tcggcgccac gtaggggcgc tcctcggccc
     9301 tcccctggtc cggcgccggc agggtgcggc ggtcgatctt gccgttgggc gtgagcggca
     9361 gggcgtccag ggtgacgaac agggccggca ccatgtactc gggcagccgc tccttgagga
     9421 actccttgag ctcagcggcc ttcggcccgg ccggagcgtg atgagcatgg ccgttgccgt
     9481 tgactccggc gccggccagg gggaccaggt aggccacgag ccgcttgctg ccgggggtgt
     9541 cctcccgcgc caggacgacg cactgggcaa tccccgggt
//
LOCUS       DASPJI010000470         6375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_323825, whole genome shotgun sequence.
ACCESSION   DASPJI010000470 DASPJI010000000
VERSION     DASPJI010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323825"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2798)
                     /locus_tag="VEL74_12815"
     CDS             complement(<1..2798)
                     /locus_tag="VEL74_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63456.1"
                     /translation="MATRTVWVPIFWFEIRYHLRQPLFYLVTFFLSVLLFIAGTGHGP
                     GGAAGRLHLNAPAVILELLVQGIFLVLFLMTAFVASAAVRDFDRRTSELFFSMPISRF
                     DYLTGRFAGTMVVCTLAYLIGAGALAASSFMPWLDPARVGPFRLAPFAFALGVLIIPT
                     LLALGAAFYALATWTRSTIATYFGVVAFFAASTTAGMAASRLETEGIGQLLDPFGVAA
                     LRGALEYWTVAELNTAIPEVGGTLLWNRILWLGMGLSALGLSVATFDPSRPRKHPGRK
                     RDSTEAPPPVEAAIRERAQAARQVFSRYSVLLQLLRQVRHEATTVLGNLPFLGILAFG
                     LFVLIQAASSAGNLFGMPVHPRTHLMLEALQGGYSILLLIVVVLYSGELIWKERSLGL
                     AEIHDSMPTPSGVYFGAKLTALLLVIAVFLFAGVLALAGFQLSRGHHGLEPGLYARGA
                     AAAAVYPSLMMVLACFCHVMARSRLAGYGLVILFILSWDLLEEFGFEHHLYRFASLPP
                     APYSDFNGYGPSVAAFGWHSLYWGFLALVLAGLSTLFWKRGTDDAWRARWAGVRGRFR
                     GPVRAVITLGALGFVAAGSWIFYNTNVLNEYVPSTEAAERRASYERLYRRYQDLALPR
                     IVAVRAEVDIFPGRRSVEIRGTYLLRNRSAEPLRELHVSLPERVQVNRLDLPAHDVIL
                     ADGELGYGIYRLREPLAPGGAIELGFGLTVGQRGFVNHGAEIAVLDNGTYFTRRDFFP
                     VIGYDAHRQLADPDERRRQGLEPVLHLPGSGDLPARGNTPRASDADRVDFETTVSTAI
                     DQTAVTSGELQREWVKDGRRYFHYRSEIPITHLFAYASARYEVTRGEWKGIAIEIYHH
                     PGHGGNVGRMIDAVRKSLAYYTASFGPYQHRHLRIVEFPRYVRDATSFPGLIAFSEAM
                     GFNARLDGEEAIDFPFYVT"
     gene            complement(2811..3707)
                     /locus_tag="VEL74_12820"
     CDS             complement(2811..3707)
                     /locus_tag="VEL74_12820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix transcriptional
                     regulator/GNAT family N-acetyltransferase"
                     /protein_id="HYG63457.1"
                     /translation="MSNDPIPTVRSFYRALTLRVGVFTDRFLGRGRPIAESRLIFEIG
                     REGAEVRELRSRLNLDSGYLSRLLRSLERQGLVQVAALESDLRSRRACLTAAGLAELA
                     ELDRLGDRFAEGTLEPLSEAERARLVEAMSDVEKLLNLSFTRISVEDPDSPDAKWCIG
                     QYFAELGVRFEGGFNAGRSIPADARDLRPPRGTFLVARLDGRLVGCGAVKATEPGIGS
                     IKRMWVSREVRGTGVGRRLLLALEKEAAGLGMRLLRLETNRSLHEAQALYRRNGYREV
                     PAFNDDPYADHWFEKHIGPSNT"
     gene            complement(3864..4130)
                     /locus_tag="VEL74_12825"
     CDS             complement(3864..4130)
                     /locus_tag="VEL74_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63458.1"
                     /translation="MRIARLLMVLLALSVTALSAATQSLPSRTPRVQRGGIEFQNNQN
                     KADGGYWYCYATPEIVNECSNYWECMALCEGDCGGPCDDEGGWQ"
     gene            4383..5552
                     /gene="sucC"
                     /locus_tag="VEL74_12830"
     CDS             4383..5552
                     /gene="sucC"
                     /locus_tag="VEL74_12830"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HYG63459.1"
                     /translation="MKIHEYQAKEILRRYGVATPRGRVTDDPEEAARICEELGGRCVV
                     KAQIHAGGRGKGGGVKLAKSPDEARQLAGQILGMQLVTHQTGPEGQLVSKVLIEETAD
                     IAQELYLSITLDRAREKHVIMASAAGGMDIEEVAEKDPEAIHREWIDHQIGIVPFQAR
                     SIARHLGLKGQTAAQAGKLISALVRAYLETDAALAEINPLMVTGGGDVMALDAKMSFD
                     DNAMFRHKDVVEMRDLAEENPMEVEASKFNLNYIKLDGNIACMVNGAGLAMATMDIIK
                     LYGGEPANFLDVGGGASQDAVKNAFRILVSDPNVKAVLINIFGGIARTDRIARGVVGA
                     IEELGDIKVPVVVRLEGTNVEEGRRILREAPFQFIVAEQMADAAEKVVAAAKGGN"
     gene            5556..>6375
                     /gene="sucD"
                     /locus_tag="VEL74_12835"
     CDS             5556..>6375
                     /gene="sucD"
                     /locus_tag="VEL74_12835"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HYG63460.1"
                     /translation="MPIWVDNDTRLVVQGITGKEGTFHALGCRDYGTKVVAGVTPGKG
                     GETVEGIPVFNSVEEAREATGANASMIFVPPPFAADAIMEAAAAEIELVVCITEGIPV
                     ADMLRATAFLQDCATRLVGPNCPGVISPGMAKIGIMPGHIHKPGSIGVVSRSGTLTYE
                     AVWQLTNLGLGQSTCVGIGGDPVNGTDFIDVLTLFNEDPETEGVILIGEIGGNAEERA
                     AEYIQKYFKKPVAGFIAGTTAPPGKRMGHAGAVISGGKGTAAAKIEALEAAGVKV"
BASE COUNT         1148 a         2130 c         2187 g          910 t
ORIGIN      
        1 gtgacgtaga acggaaagtc gatcgcctcc tcgccatcga ggcgggcgtt gaaccccatc
       61 gcctcggaaa acgcgatcag gccgggaaac gacgtggcat cccggacata gcgggggaac
      121 tcgacgatcc gcaggtggcg gtgctggtag gggccgaagc tcgccgtgta ataggcgagc
      181 gactttctga ccgcgtcgat catgcggccg acgttgccgc cgtgtccggg gtgatggtag
      241 atctcgatcg cgatgccctt ccactcgccc ctcgtcacct catacctcgc cgaggcgtag
      301 gcaaagaggt gtgtgatggg gatctcggat cggtagtgga agtagcgccg tccgtccttg
      361 acccactccc gttgcaactc gcccgaggtg acggcggtct ggtcgatggc cgtggagacc
      421 gtggtctcga agtcgacccg gtcggcgtca gaggcgcggg gcgtgttccc gcgggcaggg
      481 aggtcgccgc tgccgggcag gtggaggaca ggctccagtc cctgccgccg ccgctcgtcg
      541 ggatcggcga gctgacggtg agcgtcgtag ccgatgaccg ggaagaagtc ccgcctggtg
      601 aagtaggtgc cattgtcgag gacggcgatc tccgcgccgt ggttgacgaa gccccgttgc
      661 cccaccgtga ggccgaagcc gagctcgatg gcccctcccg gcgcgagcgg ctctcgcagc
      721 cggtagatgc cgtaaccgag ctcgccgtcc gcgaggatca cgtcgtgggc gggaaggtcg
      781 agccggttca cctggacccg ctccggaagg ctcacgtgga gctcccggag cggttcggcg
      841 gaccggttcc ggaggaggta ggtgccgcgg atctcgacgc tcctccggcc ggggaagatg
      901 tccacctcgg cacgcacggc gacgatccgc ggcagagcga gatcttgata ccgccggtag
      961 agccgttcgt agctcgcgcg gcgctccgcc gcctcggtgc tcggcacgta ctcgttcagg
     1021 acgttggtgt tgtagaagat ccaggagccc gcggcgacga agccgagggc gccgagggtg
     1081 atgaccgcgc ggacagggcc gcggaaccgt ccgcgcaccc cggcccaccg ggcccgccag
     1141 gcgtcgtccg tgcccctctt ccagaacagg gtcgagagcc cggcgagcac gagcgcgagg
     1201 aacccccagt agaggctgtg ccagccgaac gccgcgacag aaggtccgta gccgttgaaa
     1261 tcggaatagg gcgccggcgg gaggctcgcg aagcggtaga ggtgatgctc gaacccgaac
     1321 tcctcgagga gatcccagct gaggatgaag aggatcacga ggccgtaccc cgcgagcctg
     1381 ctccgcgcca tgacgtggca gaaacaggcg aggaccatca tcaggctcgg atagacggcg
     1441 gcggccgcgg cgccccgtgc gtacaggccg ggctcgaggc cgtggtggcc gcgcgagagc
     1501 tggaagccgg ccagcgccag gacgcccgcg aacagaaaga cggcaatcac cagcagcagc
     1561 gccgtcagct tggcgccgaa gtagacgccg ctcggtgtcg gcatcgaatc atggatctcg
     1621 gccagcccga gcgagcgttc cttccagatc agctcaccgc tgtacagaac caccacgatc
     1681 agcagaagga tcgagtaccc gccctggagc gcctcgagca tgaggtgagt gcggggatgg
     1741 acaggcatgc cgaagaggtt cccggcggag ctcgctgcct ggatgaggac gaagagcccg
     1801 aaggcgagga tgccgagaaa cggaaggttc cccaggacgg tcgttgcctc gtgccggacc
     1861 tggcgcaaga gctggaggag gacggagtat cgtgagaaca cctggcgggc cgcttgcgcc
     1921 cgctcccgga tcgccgcctc gaccggcggc ggggcttcgg tcgaatcccg cttccggccg
     1981 ggatgcttcc gcggacgcga cggatcgaag gtggccaccg aaaggccgag ggcggagagc
     2041 cccataccga gccagaggat ccggttccag aggagagtcc cgccgacctc cggaatcgcc
     2101 gtgttcagct ccgcgacggt ccagtactca agggcgcccc gcagcgccgc cacgccgaag
     2161 ggatcgagca actgcccgat cccctcggtc tcgaggcggg aagccgccat gccggcggtc
     2221 gtgctggccg cgaagaaggc gacgacgccg aagtaggtcg cgatcgtgct ccgtgtccag
     2281 gtcgccaggg cgtagaacgc cgcgccgagc gccaggagcg tcgggatgat cagcactccc
     2341 agggcgaacg cgaatggtgc cagccggaag ggccctaccc gtgccggatc gagccagggc
     2401 atgaagctgc tcgccgccag ggctccggcg ccgatcaggt aggcgagcgt gcagaccacc
     2461 atcgtgcccg cgaagcgccc ggtcaggtag tcgaaccgcg agatcggcat cgagaagaag
     2521 agctcggagg tccgccggtc gaagtcccgc acggcagcgc tggcgacgaa cgccgtcatc
     2581 aggaagagga cgaggaagat cccctggacc agcagctcga ggatcaccgc gggagcgttg
     2641 aggtggagcc tgccggccgc gccccccggg ccgtggcccg tgcccgcgat gaacagcagc
     2701 acgctcagga agaacgtgac cagatagaag agcggctgcc gaaggtggta ccggatctcg
     2761 aaccagaaga tggggaccca cacggtcctg gtcgccatgg agtgcagcgc tcaggtgttc
     2821 gaaggtccga tatgcttctc gaaccagtga tccgcatagg ggtcgtcgtt gaaggccggg
     2881 acctcccggt acccattgcg acgatagagc gcctgggcct cgtggaggct ccggttggtc
     2941 tcgaggcgca gcagccgcat ccccaggcct gccgcctctt tctcgagagc gaggagaagt
     3001 cgccggccca cgccagtgcc gcgcacctcc cgggacaccc acattcgctt gatcgagccg
     3061 ataccgggct cggtcgcctt gaccgcgccg cagcccacga ggcggccatc gagacgggcc
     3121 accaggaagg taccgcgtgg aggcctcaag tcccgggcgt cagccgggat cgacctgccg
     3181 gcattgaagc caccctcgaa gcgcacgccc agctccgcga agtactgccc gatgcaccac
     3241 ttcgcgtccg ggctgtccgg gtcctcgacg gagattctgg tgaacgagag gttgaggagc
     3301 ttctccacat ccgacatcgc ctccacgaga cgcgcgcgct cggcctcgct gaggggctcg
     3361 agggtcccct cggcgaatcg gtcgccgagc cggtcgagct ccgcgagctc ggcgaggccc
     3421 gcggcggtca ggcacgcgcg gcgactgcgc agatccgatt cgagggctgc gacctgaacc
     3481 aggccctgcc gttcgaggga acgcaacagc cggctgaggt aacccgagtc gaggttcagc
     3541 cgggagcgaa gctcccgaac ctccgcaccc tctctgccga tctcgaagat caatcgggac
     3601 tccgcgatcg gtcgcccgcg ccccaggaaa cggtccgtaa acacgccgac ccggagggtc
     3661 agcgcgcggt agaagctccg aaccgtagga atcggatcgt tcgacatccg ctgactttag
     3721 tcaggcaatt ggggcgccgt caaggcctgg cggccgggac gttcgggcgg acttcggttg
     3781 tgggggaggg ctaggtcagg ataagcaggg cgtcaacggc aggttcggtc aagccggtga
     3841 cgccccgcag tgcttcctcg tgtctactgc caaccgcctt catcatcgca gggaccgccg
     3901 caatcgccct cacacagggc catgcactcc cagtagttgc tgcattcatt gacgatttcc
     3961 ggcgtagcat agcagtacca ataaccaccg tcagccttgt tctggttgtt ctggaactcg
     4021 atcccgcctc tttggaccct gggcgtcctc gacggcaaag actgagtcgc agcggacagg
     4081 gcggtcacac tcaacgcgag cagcaccatc aacagccggg cgatcctcat acgtccctcc
     4141 ttaatggatg accttgttgt catctaagta aataggtgcg ctggcgagaa tttctgtagc
     4201 ccggcctgcc cgtgtccttg ccgcgcttgg cgccctcctc ttccggaggc ggtccccccg
     4261 tctgatacac tcccccgggc gcacgggccg gggtctgccc cggccggcgc catctctccc
     4321 ccgtttcggg ggaactccag ctcgaatccg ttctctaggg atgaccctcg gaggaggccg
     4381 atttgaagat ccacgagtac caggcaaaag agatcctgcg ccgctatggg gtcgcgaccc
     4441 cgcggggcag ggtgaccgac gacccggagg aggcggcccg catctgtgaa gagctcggcg
     4501 gccggtgcgt cgtcaaggcc cagatccacg ccggcggacg cggcaagggt ggcggtgtca
     4561 agctcgccaa gagccccgac gaggcccgcc agctcgccgg acagatcctc ggcatgcagc
     4621 tcgtcaccca ccagaccggc cccgagggac agctcgtcag caaggtcctg atcgaggaga
     4681 ccgccgacat cgcccaggag ctctacctct ccatcaccct cgaccgcgcc cgcgagaagc
     4741 acgtcatcat ggcctcggcc gccggcggca tggacatcga ggaggtggca gagaaggacc
     4801 ccgaggccat ccatcgcgaa tggatcgacc accagatcgg catcgtcccc ttccaggccc
     4861 gctccatcgc ccgccacctg ggcttgaagg gccagaccgc ggcgcaggcg ggcaagctga
     4921 tctccgccct cgtccgcgcc tatctcgaga ccgacgccgc gctcgccgag atcaacccgc
     4981 tgatggtcac cggcggcggc gacgtcatgg ccctcgacgc caagatgagc ttcgacgaca
     5041 acgccatgtt ccgccacaag gacgtggtcg agatgcggga tttggccgaa gagaacccga
     5101 tggaggtcga ggccagcaag ttcaacctca actacatcaa gctcgacggc aacatcgcct
     5161 gcatggtcaa cggcgccggc ctggccatgg ccaccatgga catcatcaag ctctatggcg
     5221 gcgagccggc caacttcctc gacgtcggcg gcggcgcctc ccaggacgcg gtcaagaacg
     5281 ccttccgcat cctggtctcc gatcccaacg tcaaggcggt cctcatcaac atcttcggcg
     5341 gcatcgcccg cactgaccgc atcgcccgcg gcgtcgtcgg cgccatcgag gagctggggg
     5401 acatcaaggt ccccgtggtg gtccgcctcg aggggaccaa cgtcgaggag gggcggcgca
     5461 tcctgcgcga ggcgccgttc cagttcatcg tggccgagca gatggccgac gccgccgaga
     5521 aggtggtggc cgcggcgaag ggagggaact gaaccatgcc catctgggtg gacaacgata
     5581 cgcgcctggt ggtgcagggc atcaccggca aggaggggac cttccacgcc ctcggctgcc
     5641 gcgactacgg caccaaggtg gtggccggcg tcactccggg caaggggggc gagacggtgg
     5701 aggggatccc ggtcttcaac tccgtcgagg aggcccgcga ggccaccggc gccaacgcgt
     5761 cgatgatctt cgtgccgccg ccgttcgccg ccgacgccat catggaggcc gccgccgccg
     5821 agatcgagct ggtggtctgc atcaccgagg gcatcccggt ggccgacatg ctgcgcgcca
     5881 cggcgttcct gcaggactgc gcgacgcggc tcgtcgggcc gaactgcccg ggcgtcatct
     5941 cgccgggcat ggcgaagatc ggcatcatgc cgggccacat ccacaagccg ggctcgatcg
     6001 gtgtcgtcag ccgctcgggc acgctgacct atgaggcggt ctggcagctc accaatctgg
     6061 gcctcggcca gtcgacctgc gtcggcatcg gcggcgaccc ggtcaacggc accgacttca
     6121 tcgacgtgct cacgctcttc aacgaggacc ccgagaccga gggcgtgatc ctcatcggcg
     6181 agatcggcgg caacgccgag gagcgcgcgg cggagtacat ccagaagtac ttcaagaagc
     6241 cggtcgcggg cttcatcgcc ggcaccaccg cgccgcccgg caagcgcatg ggccacgccg
     6301 gcgcggtgat cagcggcggc aagggcacgg cggcggcgaa gatcgaggcg ctcgaggcgg
     6361 ccggcgtgaa ggtcg
//
LOCUS       DASPJI010000471        10440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_773183, whole genome shotgun sequence.
ACCESSION   DASPJI010000471 DASPJI010000000
VERSION     DASPJI010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10440)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10440
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773183"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VEL74_12840"
     CDS             complement(<1..799)
                     /locus_tag="VEL74_12840"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG63461.1"
                     /translation="MGDGMTRRQELARLLRALSGKTQEDFAEAIGSHPSLIGLIEIGR
                     ADPGDTVLRTMARAAGLDLADARLLLDEHERLARFRRQRLNGEVDLMSALPERLRDHL
                     SAALRRLWTLPRPVPLPCTGDREKAHEQWRLLEAVPATLRQAVTRADKELWNWALCEL
                     LCEQSEKAAGRSVEEAAGLAQLAEEIASLVRGPEGFCRRLQGYTAAHRSNVHRVAGEL
                     KPARVALERAKALWHSGDDPFGLFDPGRLLDLEASLCRGERRFEQAVS"
     gene            905..1150
                     /locus_tag="VEL74_12845"
     CDS             905..1150
                     /locus_tag="VEL74_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63462.1"
                     /translation="MNRILVAVALTASLGTPLSSAPLDFLWSALSSFREAPASSKEGF
                     GWDPNGIHSPPPAGTDAGFDWDPDGLNGDSSAPAICS"
     gene            complement(1517..2992)
                     /locus_tag="VEL74_12850"
     CDS             complement(1517..2992)
                     /locus_tag="VEL74_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63463.1"
                     /translation="MSRKLTLRQALLWLLALRADISHKEIGAKIGISEARVSQFLRRE
                     RRLEIKDEDFEKLLGAIPHKPAEVTILTELLEALEALEDTGGLTDEQAAGVELEVLQG
                     ARLMREDLREVLRRVTSLTFDGYPEPADLTPARLLAEEQWERLRGLTPEARRAVVQGS
                     ERFQSYALCEKICDLSEEAAARDLEEAATLAGLAREIAERVRGPETWREAMMGYATAL
                     GANILRVKGELIEARVTMEEAKRLWRRGSDPAAVLDPGRLLDLEASLCRAERRCGEAL
                     GLLDEAVAVGRHPERVLIKKGFTLEVMGEYERAAGTLLRAIDRIDRRTEPRLWNIGRL
                     NLANNYSHLGRFQEAEDLVRTVRPFVIERGDKLDLIRLGGIEARLAAALGQPEEALRR
                     LRQARQQFAAQQMVYDVALAALEEAVLLLDLDQVDHVRRLGGELEEVFASRGVHREAL
                     AALRLFHEAVEREEASAELARRVLRYLYRARYDEDLPFNAS"
     gene            3384..3677
                     /locus_tag="VEL74_12855"
     CDS             3384..3677
                     /locus_tag="VEL74_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63464.1"
                     /translation="MANLPEVLCRNREAMRRLLASYVVPPWEAEPILLSVCQSMTRRQ
                     WREVAEPDRVLLQLLDDACQTFAALRRWHSMEAALDTLAAERGGAGDGAAAGV"
     gene            3682..3942
                     /locus_tag="VEL74_12860"
     CDS             3682..3942
                     /locus_tag="VEL74_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63465.1"
                     /translation="MTSRNGFVYRGGDREGATRCYRVAVRDAAETGEVSAVLMVDSVV
                     IERWERSTAKSVRLDFLPEWIERLVIRAGKRLHFFGMEEDKG"
     gene            3939..4220
                     /locus_tag="VEL74_12865"
     CDS             3939..4220
                     /locus_tag="VEL74_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63466.1"
                     /translation="MSELFEVRTQITKLVEQLITIRYQMKGVQASVPASSVESSLDDL
                     DAVSDVPTEIRTVIGNCINDSLDPLIRDLLDLVKQEPGAALANGEAGRG"
     gene            4217..4390
                     /locus_tag="VEL74_12870"
     CDS             4217..4390
                     /locus_tag="VEL74_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63467.1"
                     /translation="MSDAGILKRWINRLMKEINGLTRQLAALGDELPAAGEAVGGRFR
                     PPFQLDADRGEGC"
     gene            5034..>10440
                     /locus_tag="VEL74_12875"
     CDS             5034..>10440
                     /locus_tag="VEL74_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63468.1"
                     /translation="MTGRSPSGRPGSTLVDLLRLRAEERGEARAYTFLADGEAEEGTL
                     SYAGLDRQARTIGAALARLSAAGERVVLLFPPGLEFIAAFFGCLYAGAVAVPAYPPRS
                     QRGLPRLLSILSDARPRVVLTTRALLGKTRPLLARLAADGVAVDCLATDDLAPEGAEA
                     WTAPDIDGGTLAFLQYTSGSTSTPKGVMVSHANLLHNEEIIRQAFGQSAESVILGWLP
                     LYHDMGLIGNVLQPLYVGAPCILMSPVAFLQKPLRWLEAIGRYRATTSGGPDFAYALC
                     AARIGEEQRAGLDLSSWRVAFNGAEPVRAETMERFAAAFASCGFRREAFYPCYGLAEA
                     TLFVTGGTLGDPPRAVPFDAAALEKDRAVPPEPGRAARELVGCGQAWGGQEVLVVDPA
                     TSAARPAGTVGEVWVAGPSVAHGYWRNPEATERDFHARLADEPEAGPFLRTGDLGFFQ
                     DGELFVTGRLKDLIILRGRNLYPQDVELTAERAHPALRPGCGAAFAVEAGGAERLVVT
                     LEMERRADGASPEAVAEAVRRAVAEEHEAQVHEVVLLRAGTIPKTSSGKIQRHACRAG
                     YLAGGLEVVGRSALDPAAPGETETVLSRESLLALEPGERPAVLEAWLRDRAARLLRTS
                     SARIEPGLPLTAAGLDSLAAVELRQAVEQDLGATVPLTGLLEGATVAEIAAGVLEDLA
                     GGGLAAGAALVPGEPAAEFPLSHGQQALWFLQRLAPGSAAYHITAAVRVAGDLDVPAL
                     HRAFQALADRHPALRTTFHAGEGGEPFQRVHERLEVGFVLEDAAGLGEGVLAGRLAAF
                     AHAPFVLETGPLLRAAVFALGEAGHALVLAVHHLVADFTSLSVAFGELARLYELEAAG
                     APSQLARLDLAPLPVSYGDYVRWQAGRFAGPEGERLWEHWRQALAGPPPDLDLVTDRP
                     RPSVQTDRGASMALALGPDLSLAVRELARSSGATLFTTLLAAFQTLLHRYTGQWDFAV
                     GAPVAGRNAPELADLVGYFVNPVVLRAPLGDEGADSTFAAFLGRVRRVALEAFEHQEL
                     PFALLAERLQPSRDPSRSPLFQAMLVLQTARRPGQEALAPFALGESGAEISLGPLALT
                     SLSLPERPAQLDLTMALAEGTGEGRGGLAVSLQFNADLFDPATAGRMLGHFRTLLAAA
                     AAAPERTLGDLPLLAAAERDQLLVEWNATAEEYPRERLLHELFEEQAERTPDRVALES
                     GSLALTYRELESRANRLANHLIRLGITPETPVGVHLERSADVAVSVLALLKSGAEYLP
                     LDPAYPPERVAYTLEDAGAPLVLTLQHLEPSLDKYVKDGGRRLRLDADWREVARERAV
                     RPGLPAGRADPESAAYLIYTSGSTGLPKGVQVRHRGVVNFLSSMARRPGLGAGDVLAA
                     VTTLSFDIAGLELYLPWTVGARVVLVSREDAADGTRLLALLRRTGATAMQATPSTWRL
                     LLAAGWEGDPPITALCGGEALPRDLAEALLARAPSVWNLYGPTETTIWSAVHQVLPEE
                     TAPVAPLGRPIANTGIYIVDAALRPAPTGVPGELLIGGDGLARGYFRRPALTAERFVP
                     DGVSGAAGARLYRTGDLARWRAGGTLEFLGRLDHQVKIRGHRVELGEVEAALTSHPAV
                     AQAVVAARQDPTGGQRLVAYLVGPHPPAPSPASPPPSPGEGEVSTAELRRFLAERLPE
                     AFVPSLFVTLPAFPLTPNGKVDRKALPAPELVRREGPREAPRSEVERAVAAVWKEVLG
                     IPEVGLHDNFFDLGGHSLLAAQVHARLLRSLGRDLSLVDLFRYPTVATLARHLSPEEA
                     APAPAAPVSKREPAAGRD"
BASE COUNT         1402 a         3781 c         3627 g         1630 t
ORIGIN      
        1 gggacacagc ttgctcgaag cgtcgctcac cgcggcacag ggaagcttcg aggtcgagca
       61 gtctccccgg atcgaacagg ccaaaaggat cgtcgccgga atgccagagg gccttagccc
      121 gctcgagcgc caccctcgcc ggcttcagct ccccggcgac ccggtggaca ttggacctgt
      181 gagccgcggt atacccctgg agccgccggc agaagccttc cggcccccgc accagactgg
      241 cgatctcctc tgcgagttgg gctaagccag ccgcctcttc gacgctgcgg cccgccgcct
      301 tttccgactg ctcacagagc aactcgcata gggcccaatt ccacagctcc ttgtcagccc
      361 gggtcaccgc ctgccgtaag gtagccggaa cggcctccaa cagcctccac tgctcatggg
      421 ctttctcccg gtctccggtg caggggaggg ggaccggccg gggcaaggtc cacagtcgcc
      481 gaagcgccgc ggagagatga tcacggagtc tctccggtaa agcgctcatc aggtccacct
      541 ctccgttcag ccgctgccgc cggaaccgtg ccagtctttc gtgttcgtcc aggaggaggc
      601 gcgcgtccgc tagatcgagg ccggcggccc gggccatcgt ccgtagcacg gtatcgcccg
      661 gatcggccct gcctatctcg atcaggccaa tcaacgaggg gtggctgccg atcgcctcgg
      721 cgaagtcttc ctgggtcttg ccggacaggg cgcggagcag ccgtgccagt tcttgccgtc
      781 gtgtcatccc gtctcccacc gagaaaccgg tcgatttctt gccacttcgg acaccctact
      841 ctatgagcgc agcccgcgcg ggccggagtg gcccgggtcc ggtgccgcgc ggagaccccg
      901 gctcatgaac cgcatcctcg tcgccgtcgc cctcaccgcc tccctcggta cgcccctctc
      961 gtccgctccg ctcgacttcc tctggtcggc cctcagttcc ttcagggagg ctccggctag
     1021 ctcgaaggag ggtttcggct gggacccgaa cggcatccat agcccgccgc cggccgggac
     1081 cgacgcaggt ttcgactggg atcctgacgg tctgaacggc gacagctccg caccggcaat
     1141 ctgctcctga ttttcgccac ccctgagcaa ctaggcaagt tctagccact ccaggcacct
     1201 attctatgag cgcggcccgc gcgggccggg ttgacccgcg tccggcgccg tctggagact
     1261 ccggcttatg aaccgcattc tcgttgccgt cgccctcacc gcctccctcg gagtgcccct
     1321 ttcggccaat ccactcgatc tcctctggtc ggctctcggc tctctttggg aggctccggc
     1381 cactccaaag gaaggtttcg gctgggaccc gagcggcctc aacagcccac cgccgccggc
     1441 cgagaccgac gcgggcttcg gttgggaccc cagtggtctc aatggcagta gcccggctcc
     1501 ggcggtcggc tccggctcat gaagcattga acgggaggtc ctcgtcgtag cgcgctcggt
     1561 agaggtagcg caggacgcgc cgggccagtt cggccgaagc ctcctcgcgt tccaccgctt
     1621 catggaagag ccgcagggcg gcgagggcct ctcggtgcac gcccctggag gcgaacacct
     1681 cttccaactc cccacccagc cgccgaacgt gatccacctg gtccaggtcc aagaggagaa
     1741 ccgcctcttc tagcgcagcc agggcgacgt cgtagaccat ctgctgagcg gcaaattgct
     1801 gacgggcctg cctcaatcgc cggagggctt cctccggctg gcccagagcg gcggcgaggc
     1861 gcgcctcgat gccgccgagc cggatgagat cgagcttgtc gccgcgctcg atcacgaagg
     1921 ggcgtacggt cctcacgagg tcctcagcct cctgaaacct gccgaggtgg ctgtagttgt
     1981 tcgccagatt gaggcggccg atgttccaga ggcgcggctc ggtgcggcgg tcgattcggt
     2041 cgatcgcccg gaggagcgtc ccggcggccc gctcgtattc gcccatcacc tccagcgtga
     2101 accccttctt gatgaggacg cgctccgggt gccggcccac cgccactgcc tcgtcgagga
     2161 ggccgagggc ctcgccgcac cggcgctcag cccggcagag ggaggcttcc agatcgagca
     2221 gccggccggg atcgagcact gcggcggggt cggaccctcg ccgccagagg cgtttcgcct
     2281 cctccatggt gacccgggcc tcgatgagct cgcccttcac ccggaggatg ttcgctccga
     2341 gcgcggtggc gtagcccatc attgcctcgc gccaggtttc cggcccccgg acccgctcgg
     2401 cgatctcccg cgcgagcccg gccagggttg ccgcctcttc caggtcgcgg gcggcggcct
     2461 cttctgaaag gtcacagatc ttctcgcaga gcgcatagct ctggaaccgc tcggacccct
     2521 gcacgacggc ccgccgggcc tcgggggtga gcccccgcag gcgctcccac tgctcttcgg
     2581 cgaggagccg ggcgggcgtg agatcggcgg gctcggggta gccgtcgaag gtgagggagg
     2641 tcacccggcg gaggacctct cgcagatcct cccgcatcag ccgggcaccc tggagcacct
     2701 ccagttcgac gccggcggcc tgctcatccg tgaggccgcc ggtgtcttcc aaagcctcca
     2761 gcgcttcgag gagctcggta aggatcgtca cctcggccgg tttgtgaggg atggccccga
     2821 gcagcttctc gaagtcctcg tccttgatct ctagccgccg ctcgcgccgc aggaactggg
     2881 agacgcgggc ctcagagatc ccgatcttcg cgccgatctc cttgtggctg atgtcggccc
     2941 gtagcgccag gagccacaac agcgcctgcc ggagggtcag cttccgcgac attcaagggc
     3001 ctccttctga gattggcttc caaagaatcg agtgcgtgcc ggcgccaaaa agggacatgc
     3061 acgggcatta tcttatgaga ttcagaactt gccgcgctac taggcagagc cggccgccgg
     3121 gcacgcgatc caaatcagcc agttcgttaa gggagcgctt cagctctggc cgccctgcct
     3181 cacgattccg gtcattgtga agacggacgg ggccacttcg agaacgccgc atcagaggca
     3241 cccgagaccg gacgttctcc agcgattctt ccaaggggaa tcctcgcgcg aggagcgaag
     3301 cgtgatcgtg cgccacctgc tggccggctg ccgggagtgc tcccgggtca ctcggaagat
     3361 ctggcggctc ggagaccaga ccgatggcga acctgcctga ggtcctgtgc cgcaaccggg
     3421 aagccatgcg ccggctgctc gcctcgtacg tggtcccgcc ctgggaggcg gagccgatcc
     3481 ttctgagcgt ctgccagtcg atgacccggc ggcagtggag agaggttgcc gagccggacc
     3541 gggtcctcct gcagcttctg gacgacgcct gccagacctt cgcggcgctc cgccgctggc
     3601 attccatgga ggcggcgctg gacacgctgg cggcagagag gggaggggca ggagatggcg
     3661 ccgccgccgg tgtatagcgt cttgacctcc cggaacgggt tcgtctaccg gggcggtgac
     3721 cgcgaggggg ccacccggtg ctaccgggtg gcggtccggg acgcggcaga gacgggcgaa
     3781 gtgtcggcgg tcctcatggt ggactccgtg gtgatcgaac ggtgggagcg ctccaccgcc
     3841 aagagcgtac gtctggattt cctgccggag tggatcgagc ggctggtgat ccgagccggc
     3901 aagaggctgc atttctttgg aatggaggaa gacaaggggt gagcgagctt ttcgaagtga
     3961 ggacacagat cacgaagctg gtggagcaat tgatcacgat ccgctatcag atgaagggcg
     4021 ttcaggcgag cgtgcccgcg agctccgtgg aaagctctct ggatgacctc gacgccgttt
     4081 cggacgtgcc gacggagatc cggacggtca tcggcaactg catcaacgac tcgctcgatc
     4141 cgctgatccg ggaccttctc gatctggtga agcaggagcc cggcgctgcc ctggcgaacg
     4201 gcgaggccgg ccggggatga gcgacgccgg aatcctcaag cggtggatca accggctgat
     4261 gaaggagatc aacggcctca cccggcaact cgccgccctc ggcgacgagt tgccggcagc
     4321 aggcgaggcc gtgggaggcc gcttccgccc tccgttccaa ctagatgcag accgtggcga
     4381 gggttgttga gacagaatgc ctcctcccca agcaggccgt tttcccgggc catctccact
     4441 ttcgcccagc cttcacccca ttactgtcag ccctctcgtt ctttctcgaa gcctgcggca
     4501 aggtagtgct ggaacgcgcc ggcgccatcc tggaagagcg ggtgacggcg acgcccggct
     4561 gatcctggcc cccttcgtga cggggtgacg tcttcgcgcc gttccccccg ggaggagcct
     4621 ttctcccggg gtttttcttg accccatatg gggatcctgt acaggatggc cccatgagag
     4681 ccattttccc tgacatcgag gacgaggttt atcggagtct gatgaacctc ggtcgtgaag
     4741 cccaggacct gggatgagct cttgatgaca tgctcgaccc ttgatcctgg ctgttgacac
     4801 ggacgtcctg atggcatggg cgacggtgct gagcccgggc cacgttatga gactcctctc
     4861 gcggccggga cccgccttgt cagtgtcacg ccttcgtgac aggctgcact ctaggtatat
     4921 ggacccgtct cgggtccgcc gcccctcgtg tcggcgcgcg gccggcccgg gggaggcaga
     4981 ggacgagcgc tcttggtgtt tttttcaacc cccctagccg gaccaatccg cggatgactg
     5041 gacgcagccc ctcaggccgg cctggctcga cgctggtcga tctgctccgc ctgcgcgccg
     5101 aggagcgggg ggaggcgcgt gcctatacct tcctcgcgga cggcgaggcc gaggagggga
     5161 ccctctctta tgccgggctg gaccgccagg ccaggaccat cggcgccgcc ctcgcccgtc
     5221 tgtcggcggc cggcgagcgg gtggtgctgc tcttcccgcc gggcctcgag ttcatcgccg
     5281 ccttcttcgg ctgcctctac gccggtgcgg tggcggtgcc cgcctatccg ccgcggtccc
     5341 agcgtggcct ccctcgtctg ctgtcgatcc tgagcgacgc ccggccgcgg gtggtgctga
     5401 ccacccgggc gctcctcggc aagacccggc cgctcctcgc ccggctcgcg gcggacggcg
     5461 tcgcggtcga ttgcctggct accgatgacc tggcgcccga gggggcggag gcctggacgg
     5521 caccggacat cgatggcggc accctggcct tcctgcagta cacctcgggt tccacctcca
     5581 cccccaaggg ggtcatggtg agccacgcca acctcctcca caacgaggag atcatccgcc
     5641 aggcgttcgg ccagtcggcg gagtcggtga tcctggggtg gctccccctc taccacgaca
     5701 tggggctcat cgggaacgtg ctgcagccgc tctacgtggg cgcgccctgc atcctgatgt
     5761 cgcccgtcgc cttcctccag aagccgttgc gctggctcga agccatcggc cgctaccggg
     5821 cgaccaccag cggcggcccg gacttcgcct atgccctgtg cgcggcgcgg atcggggagg
     5881 agcagagagc cgggctcgac ctctcctcct ggcgcgtcgc cttcaacggc gccgagccgg
     5941 tgcgggccga gaccatggag cggttcgccg ccgccttcgc ctcctgcggc ttccgccgcg
     6001 aggcgttcta tccctgctac ggcctcgccg aggcgaccct gttcgtcacc ggggggacgc
     6061 tgggcgatcc gccgcgggcc gtgccgttcg acgccgccgc tctggagaag gaccgggcag
     6121 tgccgccgga gcccggcagg gccgcgcgcg agctggtggg ctgcggccag gcgtgggggg
     6181 gccaggaggt gctggtggtg gaccccgcca cctccgcggc ccgccctgcg ggcacggtgg
     6241 gggaggtctg ggtcgccgga cccagcgtcg cccatgggta ttggcgcaat cccgaagcca
     6301 ccgagcggga cttccacgcc cgcctcgccg acgagccgga ggccgggccg ttcctgcgca
     6361 ccggcgacct cgggttcttt caggacggcg agctgttcgt caccggccgg ctcaaggacc
     6421 tgatcatcct gcgcggccgg aacctctatc cccaagacgt ggagctcacg gcggagcggg
     6481 cccacccggc cctgcgcccg gggtgcggcg ccgccttcgc cgtcgaggcc ggcggcgcgg
     6541 agcggctggt cgtcaccctg gagatggagc gccgggcgga cggcgcgagc cccgaggcgg
     6601 tggcggaggc ggtccgccgc gccgtggccg aggagcacga ggcccaggtc cacgaggtgg
     6661 tcctgctccg cgccggcacc atccccaaga cctccagcgg caagatccag cgccacgcct
     6721 gccgggccgg ctacctcgcc ggcggcctgg aggtggtggg acggagcgcc ctcgatcccg
     6781 ccgcccccgg cgagacggag accgtcctct cccgggagtc gctcctcgcc ctggagcccg
     6841 gcgagcgccc cgctgtcctg gaagcctggc ttcgagaccg ggcggcgcgg ctgctgcgga
     6901 cctccagcgc gcgcatcgag cccggcctgc cgctcaccgc cgccgggctc gactcgctgg
     6961 ccgcggtgga gctgcggcag gcggtggagc aggacctcgg cgccaccgtg ccgctcaccg
     7021 gtctcctgga aggggccacg gtggcggaga tcgccgccgg cgtgctggag gatctggcgg
     7081 gaggggggct ggccgcgggg gctgccctgg tgcccgggga gccggcggcc gaattcccct
     7141 tgtcccacgg ccagcaggcc ctctggttcc tgcaacgcct ggcccccgga agcgccgcct
     7201 accacatcac cgcggccgtg cgcgtggccg gcgatctgga cgtgccggcg ctccaccggg
     7261 cgttccaggc cctggccgac cggcaccccg ccctgcgcac caccttccac gctggggagg
     7321 ggggggagcc gttccagcgg gtccacgaaa ggttggaggt gggcttcgtc ctggaggacg
     7381 ccgccgggct gggggagggg gtcctcgccg gccgcctcgc cgccttcgcc cacgcgccgt
     7441 tcgtcctcga gaccgggccg ctgctgcgcg ccgccgtctt cgcgctgggg gaggccggcc
     7501 acgctttggt gctggcggtt catcacctgg tcgcggactt cacctcgctc tccgtcgcct
     7561 tcggcgagct ggcccggctc tacgagctgg aggccgccgg cgccccgtcc cagctagccc
     7621 ggctcgacct cgcgccgctg ccggtgtcct atggcgacta cgtgcgctgg caggccgggc
     7681 ggttcgccgg tcccgaaggg gaacggctgt gggagcactg gcggcaggcg ctcgccggac
     7741 cgccgccgga cctggacctg gtcaccgacc ggccgcgccc gtcggtgcag accgaccgcg
     7801 gcgcctccat ggctctggcg ctggggccgg acctctctct agcggtgcgg gagctggccc
     7861 ggtcgtccgg ggcaaccctc ttcaccacgc tgctggcggc gttccagact ctcctccacc
     7921 gctacaccgg gcagtgggac ttcgcggtcg gcgcgcccgt ggccggccgc aacgcgccgg
     7981 agctggcgga cctggtgggc tacttcgtca atcccgtggt gctgcgcgcg ccgctggggg
     8041 acgaaggggc cgattccacc ttcgccgcct tcctggggcg ggtccggcgc gtggcgctgg
     8101 aggcgttcga gcaccaggag ctccccttcg ccctgctggc cgagcgcctg cagcccagcc
     8161 gcgaccccag ccggtcgccg ctcttccagg ccatgctggt gctccagacc gcccgccggc
     8221 cggggcagga ggcgctcgcc cccttcgccc tcggcgagtc cggggcggag atctccctcg
     8281 ggccgctggc cctcacctcc ctatccctgc ccgaacggcc ggcgcagctc gacctcacaa
     8341 tggcgctggc cgaaggaacg ggggagggga ggggcggcct cgccgtctcc ctccagttca
     8401 acgccgacct gttcgacccg gccacggccg gccgcatgct ggggcacttc cggacgctcc
     8461 tcgcggccgc cgccgccgct ccggaacgga ccctgggcga cctccccctg ctcgcagcgg
     8521 ccgagcgcga ccagctcctg gtggagtgga acgccacggc agaggagtac ccgcgggagc
     8581 gcctcctcca cgagctgttc gaagagcagg cggagcgcac gccggaccgg gtggccctcg
     8641 agtccggctc gctggccctc acctaccggg agctggagag ccgggccaac cggctcgcca
     8701 accatctgat ccgtctgggg atcacccccg agacgccggt gggggtgcac ctggagcgct
     8761 cggccgacgt ggccgtgagc gtcctcgccc tcctcaagag cggcgcggag tatctgccgc
     8821 tcgatccggc ctacccgccc gagcgcgtcg cctacacgct ggaggacgcc ggagcccctc
     8881 tggtcctgac cctccagcac ctggagccct ccctcgataa gtatgtaaag gatggaggcc
     8941 gccggctgcg tctggacgcg gactggcgag aggtcgcccg ggagcgcgcg gttcgcccgg
     9001 gattgccggc agggcgggcg gacccggaga gcgccgccta cctcatctat acgtccggct
     9061 cgaccggcct ccccaagggg gtgcaggtgc ggcaccgcgg ggtggtcaac ttcctgtcct
     9121 ccatggcgcg gcggccgggc ctcggcgccg gcgatgtgct ggccgcggtc accacgctct
     9181 cgttcgatat cgccggcctg gagctgtacc tgccgtggac cgtgggggcg cgggtggtcc
     9241 tggtgtcgcg ggaggacgcg gcggacggca cccggctgct cgcgctgctg cgccggacgg
     9301 gggccacggc catgcaggcg acgccctcca cctggcgcct gctcctggcc gccggctggg
     9361 agggggatcc cccgatcacg gccctctgtg gcggcgaggc gcttccccgg gacctggccg
     9421 aggcgctcct ggcgcgcgcg ccgtcggtct ggaacctcta cgggcccacc gagaccacga
     9481 tctggtcggc ggtgcaccag gtgctgcccg aggagacggc ccccgtcgcc cccctgggcc
     9541 ggccgatcgc gaacaccggc atctacatcg tggacgccgc gctgcggccc gcgcccacgg
     9601 gcgtgcccgg agagctgctc atcggcggcg acggcctggc ccggggctat ttccggcgcc
     9661 cggccctcac cgccgagcgc ttcgtccccg atggggtgag cggcgccgcc ggggcgcggc
     9721 tctaccgcac gggcgacctg gcccgctggc gcgccggcgg caccctggag ttcctgggcc
     9781 ggctcgacca tcaggtgaag atccgcggcc accgagtcga gctgggcgag gtggaggcgg
     9841 cgctgacctc ccaccccgcg gtagcgcagg cggtggtcgc cgcccgccag gatcccacgg
     9901 gcggacagcg gctggtggcg tacctggtgg gccctcaccc cccggccccc tctcccgcct
     9961 ccccgccacc ctccccggga gagggggagg tctccaccgc tgagctgcgc cgcttcctgg
    10021 ctgagcggct gcccgaagcg ttcgttcctt ctctgttcgt caccttgccg gcgttccccc
    10081 tgacgcccaa cggcaaggtt gaccgcaagg ccctgccggc gccggagctg gtacggcgcg
    10141 aagggccgcg ggaggccccg cgctcggagg tggagcgggc ggtcgccgcg gtgtggaagg
    10201 aggtcctggg catccccgag gtggggctgc acgacaactt cttcgacctc ggcggccact
    10261 cgctcctcgc cgcccaggtg cacgcgcggc tgctgcgctc cctgggacgc gacctgtcgc
    10321 tggtcgacct cttcaggtat ccgacagtgg ccactctggc ccgccacctg agcccggagg
    10381 aggcggcgcc cgcgccggcg gctcccgtgt cgaagagaga gccggcggcg ggcagggaca
//
LOCUS       DASPJI010000472         8357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_374502, whole genome shotgun sequence.
ACCESSION   DASPJI010000472 DASPJI010000000
VERSION     DASPJI010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8357)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8357
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374502"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="VEL74_12880"
     CDS             complement(<1..1342)
                     /locus_tag="VEL74_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HYG63469.1"
                     /translation="MLKPDPVFEVPIPDRPAGLEARTAALLAALDQRVLVLDGATGTA
                     LQGVRLTAEDFGGPELEGCNENLCATRPDVVDGVHEAYLSAGCDIVETNSFGGTPLVL
                     AEYGLAHKAFELNAASARIAREACARHDRPGKERFVCGSMGPTTKAISVTGGITFEEL
                     IEHFRVQALGLMAGGTDYLLVETAQDTRNIKAALIGIERAFELAGWRIPVAVSATIET
                     TGTMLAGQDAEALAVSLLHADLLYVGLNCATGPGLMTDHLRTLSEICRTRVACVPNAG
                     LPDEEGLYTEGPEDFEKVFGRFLDAGWLNLVGGCCGTTAAHVSALSRLVEGRAPRRIP
                     HHRRSLVSGLEAVELTEDNRPLLVGERTNVLGSLKFKKLIQAGQYEAAAEVGRAQVRS
                     QAQILDVCLQDPDRNEIGDMEAFLDRLVRMVKVPLMIDSTDAAVMATALTYCQGK"
     gene            1664..1861
                     /locus_tag="VEL74_12885"
     CDS             1664..1861
                     /locus_tag="VEL74_12885"
                     /inference="COORDINATES: protein motif:HMM:NF038153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I lanthipeptide"
                     /protein_id="HYG63470.1"
                     /translation="MKKKSKKLGLSRETLTNLTPGALEQAGGGAPATWYNCPCQESVL
                     VCSIQKTCVSCVATDTCTVEA"
     gene            2003..2191
                     /locus_tag="VEL74_12890"
     CDS             2003..2191
                     /locus_tag="VEL74_12890"
                     /inference="COORDINATES: protein motif:HMM:NF038153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I lanthipeptide"
                     /protein_id="HYG63471.1"
                     /translation="MKKKTKKLGLTRETLTNLNSASLEHAAGAVSGGHKTCCQESNVI
                     CSVQHTCVSCVATDATCA"
     gene            2217..2399
                     /locus_tag="VEL74_12895"
     CDS             2217..2399
                     /locus_tag="VEL74_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I lanthipeptide"
                     /protein_id="HYG63472.1"
                     /translation="MKKKAKKLGLHRETLAHLAPEALGLVAGQATYLNCPCQESILVC
                     SEVHTCVSCVQSEDCG"
     gene            2433..2633
                     /locus_tag="VEL74_12900"
     CDS             2433..2633
                     /locus_tag="VEL74_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63473.1"
                     /translation="MKKKAKKLGLTRETLTNLNSEALGLAGQAAVAPVAGTFFNCPCQ
                     ESQMICSVMHTCVSCNRTETWA"
     gene            2665..2889
                     /locus_tag="VEL74_12905"
     CDS             2665..2889
                     /locus_tag="VEL74_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63474.1"
                     /translation="MKKKAKKLGLTRETLSNLAPESLQIAAARALATDPAGPAVGTWL
                     NCCQESIMVCSVRESCVSCNTTGGTIIAVG"
     gene            complement(2958..3908)
                     /locus_tag="VEL74_12910"
     CDS             complement(2958..3908)
                     /locus_tag="VEL74_12910"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HYG63475.1"
                     /translation="MKVTEHLAQADRPLISFEIIPPLRGGDVQGLLALIDQLVKYHPP
                     FIDITSHAAEVIYEETPQGIQRRVKRKRPGTLGVCALLQNKYHVDAVPHILCRGFTRE
                     ETEDFLIELRYLGIDNVLAVRGDDSGFSKPLQYGRSANVYACDLVQQIACMNRGKYLE
                     ENLLDAEPSNFCIGVGGYPEKHFEAPNLMLDARRAKEKIDAGAHYVVTQMFFNNQHYF
                     HYLDVCREQGITAPIIPGLKILTSQKQLSTLPSSFFVEIPTALADEVLAAKPEHVVDI
                     GVEWALQQSQELLERGVPSLHFYVMQSAVAINKLMAKLKI"
     gene            complement(3908..4384)
                     /locus_tag="VEL74_12915"
     CDS             complement(3908..4384)
                     /locus_tag="VEL74_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63476.1"
                     /translation="MRKWVVFGLLWALIPVVSPSRAFAAEEGSLRFETTVPFRTGKLV
                     DLKATVGPVKVRNLEFSQGQGGSGGIAGRFRPGAASETQTTLTAAFDTENPNEDEWVV
                     TYTLDFLDKDGKLIDRVSKKEGFEGEAKVYRLDHPILEYVVPFISKVKVRLEARYD"
     gene            4732..5082
                     /locus_tag="VEL74_12920"
     CDS             4732..5082
                     /locus_tag="VEL74_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63477.1"
                     /translation="MPSHSDRTPSRVDLSRPTESTRRHLYELVVRVYFHPTPEEVENA
                     QADPGNHWVPDYGPDEARLHVFYAYGRWFAVWEALEERKETGPLPEWRRWSVVRIVED
                     RDPSSATGVTFLEV"
     gene            complement(5593..6375)
                     /gene="nudC"
                     /locus_tag="VEL74_12925"
     CDS             complement(5593..6375)
                     /gene="nudC"
                     /locus_tag="VEL74_12925"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HYG63478.1"
                     /translation="MNPAFWLAFRGNELLVRVEDGLARLVAGAEWPQLGLPAVGTSHR
                     AGELGGGLCYTVELAAESEPPAGLSFIGLRALWALVDEETWRLAARAVQIVDWDRNHR
                     FCGRCATPTEIQRDEMARRCPRCGLQQFPRISPAVIVMVRRGDEMLLARSPHFAPGVY
                     STLAGFVEPGESLEQTVAREVHEEVGVTLTNIRYFGSQPWPFPNSLMIGFTADWAGGD
                     IRPQPGEIEDAGWFTVDNLPGLPSPMSIARKLVDAFLAGQRR"
     gene            complement(6372..7061)
                     /locus_tag="VEL74_12930"
     CDS             complement(6372..7061)
                     /locus_tag="VEL74_12930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG63479.1"
                     /translation="MTRGDLVVITGAGRGIGREAALQFARHRWRVLVTYHRDPGAAET
                     VAGECRRAGAPDAWAWELDVREPASIRGFSDRLFERFGEVTALVNNAGVIVWNPLSEQ
                     TEEEIRSQLETNLVGAILLTRALLPYVRETVIQVGSDLGYAGMAELAPYCATKFGLRG
                     FCQALAQERPDLRFLCVNPDRTATSMNRFQGRDPRVAGEVIWRAAAGHYGVASGGDVN
                     VWEVVAQEAAG"
     gene            7169..>8357
                     /locus_tag="VEL74_12935"
     CDS             7169..>8357
                     /locus_tag="VEL74_12935"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HYG63480.1"
                     /translation="MASFPWLTGFESTQIHGTGKDVLWGTHHLQRWRHDLELVRASGV
                     TTLRYPVPWHRIEARPGRYDWAWMDEVLAYMREAGLEPIVDPIHHTSFPEWIKGGFEN
                     PRFADLYQAFVGEFARRYPWVRKYTIFNEPFVTTLFCGSEGIWYPYARSQRRFVRMMV
                     QVGRAICGISEMLQELVPGVELIHVDTCEHHQAMDAGSREKVEFLNHRRFLFHDLITG
                     RIDEAHPLYAYLRHNGFLKKEMRWFRSHPARIDVLGLDYYSHSEQQRYRNRVRFYRAP
                     ARGFAAVAGDYIERYGLPVMLSETNIKGRVSDRLSWLKHMAEECGKVVAAGADFRGFC
                     WFPFVDSTDWCSLVCRAEKKVDAVGIYWLDDDRWARHASELSDCFGRLARGEMSVQAL
                     PSYR"
BASE COUNT         1368 a         2782 c         2905 g         1302 t
ORIGIN      
        1 acttgccctg gcagtaggtg agggccgtgg ccatcaccgc cgcgtcggtc gagtcgatca
       61 tcagcggcac cttgaccatg cggacgagcc ggtcgaggaa ggcctccatg tcgccgatct
      121 cgttgcggtc cgggtcctgg aggcagacgt cgaggatctg ggcctgggag cggacctggg
      181 cgcggcccac ctcggccgcg gcctcgtact ggccggcctg gatcagcttc ttgaacttga
      241 ggctgccgag gacgttggtg cgctcgccga ccagcagcgg ccggttgtcc tccgtcagct
      301 ccacggcctc gaggcccgag acgaggctcc gccggtggtg ggggatgcgc cgcggcgccc
      361 tcccctcgac cagccgggag agggcggaga cgtgcgccgc cgtggtgccg cagcagccgc
      421 ccaccaggtt gagccagccg gcgtcgagga agcggccgaa caccttctcg aagtcctccg
      481 gcccctcggt gtagagcccc tcctcgtcgg ggaggccggc gttggggacg caggccaccc
      541 gcgtgcggca gatctcggac agcgtgcgca ggtggtcggt catcaggccc ggaccggtgg
      601 cgcagttcag gcccacatag aggaggtcgg cgtggaggag cgacaccgcc agcgcctcgg
      661 cgtcctggcc ggcgagcatg gtgccggtgg tctcgatggt ggccgacacc gccaccggga
      721 tgcgccagcc ggccagctcg aaggcgcgct cgatgccgat cagggcggcc ttgatgttgc
      781 gcgtgtcctg ggcggtctcg acgagcaggt agtcggtgcc gccggccatg aggccgaggg
      841 cctggacccg gaagtgctcg atcagctcct cgaaggtgat gccgccggtc accgaaatcg
      901 ccttggtggt cgggcccatc gagccgcaga cgaagcgctc cttgccgggc cggtcgtggc
      961 gggcgcaggc ctcgcgggcg atgcgggcgg aggcggcgtt gagctcgaac gccttgtggg
     1021 cgaggccgta ctcggccagc accagcggcg tgccgccgaa ggagttggtc tccacaatgt
     1081 cgcagccggc ggagaggtag gcctcgtgta cgccgtcgac cacgtcgggc cgggtggcgc
     1141 agaggttctc gttgcacccc tcgagctcgg ggccgccgaa gtcctcggcg gtcagccgca
     1201 ccccctggag ggcggtgccg gtggcgccgt cgagcaccag gacgcgctga tcgagggcgg
     1261 cgagcagagc cgccgtccgc gcctcgaggc cggcggggcg gtcggggatg ggaacctcga
     1321 agaccggatc gggcttcaac acgtcactca agggatctcc tcctggcggg caacaaaaaa
     1381 cccgcctcgc atgcatcacg agacgggctt ttcgcaccgc ggacacttgg ttgccagccc
     1441 ctgttgcgtc ggatcatgac gggggcgagc cgcatcctcg cttgcgcgag agggcacctt
     1501 gggtgtccgt cccaggttgc cgagcccggc gggtgagggc cgagccgctc gtaatgcgtt
     1561 attgagctta gcggagtccg gtgggactgt aaagacggcc gcagcggccc gtcactccct
     1621 cgggctcacc accacaacct cgaacgttcg aaggagtatc cccatgaaga agaagtcgaa
     1681 gaagctcggc ctcagccgcg agaccctgac caatctcacc cccggcgccc tggagcaggc
     1741 ggggggaggc gcgccggcga cctggtacaa ctgtccgtgc caggagagcg tcctggtctg
     1801 cagcatccag aagacctgcg tcagctgcgt ggccaccgac acctgcaccg tcgaggcctg
     1861 accgtccgcg agcggggctc cccctggaag gggggccccg ctttctcttt cttccactcc
     1921 ggcgacccgc tccaccctcc ggcaagcacc atcaaattag gacacctaac gtccctccca
     1981 caagaagcag aaggagatcc ccatgaagaa gaagacgaag aagctcggcc tgacccgcga
     2041 gaccctgacc aacctcaact ccgcgtctct cgagcatgcc gccggcgcgg tgtcgggcgg
     2101 ccacaagacc tgctgccagg agagcaacgt gatctgcagc gtccagcaca cctgcgtgag
     2161 ctgcgtggcc acggacgcga cctgcgccta gagaccggcg ccagtcggag agtttcatga
     2221 agaagaaggc gaagaagctc ggcctgcacc gcgagaccct cgcccatctc gcccccgaag
     2281 ccctcgggct cgtggcgggg caggcgacct atctcaactg cccgtgccag gagagcatcc
     2341 tggtctgcag cgaggtgcac acctgcgtga gctgtgtgca gagcgaagac tgcggatagc
     2401 cggtcacctg ccggaatacc gaaggagatg ccatgaagaa gaaggcgaag aagctcggcc
     2461 tgacccgtga gaccctcacc aacctcaact ccgaagccct cgggctggcg ggacaggcgg
     2521 cggtggcgcc ggtggcgggc acgttcttca actgcccgtg ccaggagagc cagatgatct
     2581 gcagcgtcat gcacacctgc gtgagctgca accgtacgga aacctgggcc tgatcaccgg
     2641 cacccgccgc gtgaaggaga tcctatgaag aagaaggcga agaagctcgg cctgacccgc
     2701 gagacgctct ccaacctcgc ccccgaatcc ctccagatcg ccgctgcgag ggcgctggcg
     2761 accgatccgg cggggccggc ggtggggacc tggctcaact gctgtcagga gagcatcatg
     2821 gtgtgcagcg tgcgcgagag ctgcgtgagc tgcaacacca ccggcggcac catcatcgcc
     2881 gtcgggtagt ctccgggcgg cactcgggac ctgccgtgag gcccccttcc cggagggggc
     2941 ctcgttgctt caggaaatca gatcttcagc ttcgccatca gcttgttgat ggcgacggcg
     3001 ctctgcatga catagaagtg gagggagggg acgccgcgct ccaggagctc ctgggactgt
     3061 tggagggccc actcgacgcc gatgtccacc acgtgctcgg gcttggcggc gaggacctcg
     3121 tcggcgaggg cggtggggat ctcgacgaag aagctgctgg ggagcgtcga gagctgcttc
     3181 tgcgacgtca ggatcttcag ccccgggatg atcggcgccg tgatcccctg ctcccggcag
     3241 acgtccagat aatggaagta gtgctggttg ttgaagaaca tctgggtgac cacgtagtgg
     3301 gcaccggcgt cgatcttctc cttggcgcgg cgggcgtcga gcatcaggtt cggcgcctcg
     3361 aagtgcttct cggggtagcc gccgacgccg atacagaaat tggacggctc ggcgtcgagg
     3421 aggttctcct ccaggtactt gccgcggttc atgcaggcga tctgctgcac caggtcacag
     3481 gcgtagacgt tggcgctgcg gccgtactgg agcggcttcg agaagccgct gtcgtcgccg
     3541 cgcaccgcga ggacgttgtc gatccccagg tagcggagct cgatcaggaa gtcctcggtc
     3601 tcctcgcggg tgaagccgcg gcagaggatg tggggcaccg cgtcgacgtg gtacttgttc
     3661 tggagcagcg cgcagacgcc cagggtgccc ggccgtttgc gcttgacccg gcgctggatg
     3721 ccctggggcg tctcctcgta gatcacctcg gccgcatggc tggtgatgtc gatgaacggc
     3781 gggtggtact tgaccagctg gtcgatcagc gcgagcagcc cctgcacgtc accgccgcgc
     3841 agggggggga tgatctcgaa gctgatcagc ggccggtcgg cctgcgcgag atgctcggtg
     3901 accttcatca gtcgtagcgg gcttccagcc ggaccttgac cttgctgatg aacgggacga
     3961 cgtattcgag gatcgggtgg tcgaggcggt agaccttggc ctccccctcg aagccctcct
     4021 tcttgctgac ccggtcgatg agcttgccgt ccttgtcgag gaagtccagg gtgtaggtga
     4081 ccacccactc gtcctcgttg gggttctcgg tgtcgaacgc cgccgtcagg gtggtctggg
     4141 tctcggacgc ggcgccgggc cggaaccggc cggcgatgcc gccgctgcca ccctgcccct
     4201 ggctgaactc gagattgcgc accttgaccg gccccaccgt ggccttgagg tccacgagct
     4261 tgccggtcct gaacgggacc gtggtctcga aacggaggct gccctcctcg gcggcgaacg
     4321 cccgcgaggg ggagacgacc gggatcagag cccacagcaa cccgaaaaca acccacttcc
     4381 gcatactgcc tccttggcgg gggccagcgt atccgcagcc ccctccgggg tcaagagacc
     4441 atctcccgcc ccgctctgcc agaaacgccc ccgcgacgcc gatcccctct ccggaggcgg
     4501 gagaggggag gtgggcaaaa ccaacacgga ctgacacgga ccatcacgga ccatcacgga
     4561 ccggcggagc agtggcaggg ggagagaacg aaaccgaagc tgcacagccg tacaggaccg
     4621 ctgtgtctcc gaggtgagcg tgtagctcat ccggggttcc agggtccaag cggccaaggg
     4681 agggagttag cgctcggctc ccgaggccta gccggacagg agctagaact catgcccagc
     4741 cacagtgatc gtacgccttc gcgcgtcgat ctgtcccgtc ccaccgaatc cacccgccgt
     4801 catctctacg agctggtggt ccgcgtctac ttccatccca ctcctgaaga ggtggagaac
     4861 gcccaggcgg atcccggcaa ccactgggtg ccggactacg gtcccgacga ggcgcgcctc
     4921 cacgtcttct atgcctatgg ccgctggttc gcggtctggg aggcgttgga ggagaggaag
     4981 gagaccggac cgctgcccga gtggcggcgc tggtcggtgg tgcggatcgt cgaggaccgg
     5041 gacccgtcca gcgcgaccgg tgtgaccttt ctcgaggtct gacaccgcta cggaggcggg
     5101 tccctcctct cggggcctgc cgctctctcc gacgctccct tcgcggagcg ctccatctag
     5161 ataaggaagg acttccttcg ccgggacgac ctccgggccg tcccggccct cccctgcggg
     5221 ctcgctccgt accgtttccc cgcggcaggg aagtgttccg gcgcggcgcc gaagtcgtac
     5281 ggagccatcc cgtagcactt ccctgcggcc gggaagtgtt gaggaatggc gccgtagtgc
     5341 ttcgccaccg tcccgaagca gtacggagcc gttccgtagc acttccccgc ggcagggaag
     5401 cgttgaggag tcgcgccgta gtacttcgcc gccgcgccaa agtggtacgg agccgttccg
     5461 tagcacttcc ctgcgggagg gaagcgttga ggaacggcgg cgtagtgttc cggaatcgct
     5521 tcgtagtgct tcgcgcgggt cgagggtcac agcggcccgg cggagcgccg gggatggcct
     5581 cgcccagacg gctcagcgcc gctgcccggc caggaaggcg tcgaccagct tgcgggcgat
     5641 gctcatcggg gaggggaggc ccggcaggtt gtcgacggtg aaccagccgg cgtcctcgat
     5701 ctcgccgggc tgcgggcgga tgtcgccgcc ggcccagtcg gcggtgaagc cgatcatcag
     5761 ggagttgggg aacggccagg gctggctgcc gaagtagcgg atattggtga gcgtcacccc
     5821 cacctcctca tggacctccc gcgcgacggt ctgctccagc gactcgccgg gctcgacgaa
     5881 gccggcgaga gtgctgtaga cccccggcgc gaaatggggc gagcgggcga ggagcatctc
     5941 gtccccccgc cgcaccatga cgatcaccgc cggcgagatg cgggggaact gctgcaggcc
     6001 acaacgggga cagcgccgcg ccatctcgtc ccgctggatc tcggtcgggg tggcgcagcg
     6061 gccgcagaag cgatggttgc ggtcccagtc cacgatctga accgcccgcg ccgcgagacg
     6121 ccaggtctcc tcgtccacca gggcccagag cgcgcgcagt ccgatgaagg agagccccgc
     6181 gggaggctcg gactccgccg ccagctccac cgtatagcag aggccgccgc ccagctcgcc
     6241 ggcgcggtgg gacgtcccca cggcggggag acccagctgc ggccactcgg cgccggccac
     6301 cagccgggcc agcccgtcct cgacccggac cagcagctcg ttgccgcgga aggcgagcca
     6361 gaaggctgga ttcatccggc cgcctcctgg gcgaccacct cccagacgtt gacgtcgccg
     6421 ccgctcgcca ccccgtagtg gccggcggcg gcacgccaga tcacctcgcc ggcgacgcgc
     6481 gggtcccggc cctggaagcg gttcatgctc gtcgccgtcc ggtcggggtt gacgcagagg
     6541 aaccggaggt ccggccgctc ctgggccagc gcctggcaga agccgcgcag gccgaacttg
     6601 gtggcgcagt agggggccag ctccgccatg ccggcgtagc cgaggtcgct gccgacctgg
     6661 atcaccgtct cccgcacgta cggcaggagg gcccgtgtga ggaggatcgc ccccaccagg
     6721 ttggtctcga gctgcgagcg gatctcctcc tccgtctgct cgctcagcgg gttccagacg
     6781 atcacgccgg cattgttgac cagcgccgtg acctcgccga agcgctcgaa caggcgatcg
     6841 ctgaagccgc ggatgctcgc cggctcgcgt acgtcgagct cccaggccca ggcgtccggc
     6901 gctcccgccc gccggcactc cccggccact gtctcggcgg cacccgggtc ccggtgatag
     6961 gtcaccagca cgcgccaacg gtggcgggcg aactgcagcg ccgcctcccg gccgatgccg
     7021 cggcctgcgc cggtgatgac gacgaggtca ccgcgggtca cggaagtggc ttccatgttc
     7081 ccaaggactt agcgatggca ctgaacctgc acaggtacag cgcccccggc cgggaccacc
     7141 cgaaacgggc acagagacag gaggcgggat ggcttcgttt ccatggctta ccggcttcga
     7201 gagcacgcag attcatggta ctggaaagga cgtgctctgg ggcacccatc accttcagcg
     7261 ctggcggcac gacctcgagc tggtccgcgc cagcggcgtc accacgctgc gctatccggt
     7321 gccgtggcac cggatcgagg cacggccggg ccggtatgat tgggcctgga tggacgaggt
     7381 gctggcctac atgcgggagg cggggctgga accgatcgtg gacccgatcc atcacacctc
     7441 cttccccgag tggatcaagg gaggattcga gaacccgcgc ttcgccgatc tctaccaggc
     7501 cttcgtgggg gaatttgccc ggcggtaccc gtgggtccgc aagtacacca tcttcaacga
     7561 gccgttcgta actacgctgt tctgcggcag cgaagggatc tggtacccct atgcccggag
     7621 ccagaggcgg ttcgtccgca tgatggtgca ggtggggcgc gccatctgcg gcatcagcga
     7681 aatgctccag gagttggtgc cgggcgtgga gctcatccat gtcgatacct gtgaacacca
     7741 tcaggcaatg gacgcgggtt cgcgcgagaa ggtcgagttc ctgaatcaca gacggtttct
     7801 cttccacgac ctgatcaccg gcaggatcga cgaggcgcac ccgctgtacg cctatctgag
     7861 gcacaacggc tttctgaaga aggaaatgcg gtggttccgg agccacccgg cccgcatcga
     7921 cgtgctcggc ctcgactact actcccacag cgaacagcag cgctaccgga accgtgtccg
     7981 gttttacagg gcccccgccc gcgggttcgc cgcggtcgcc ggcgactata tcgagcggta
     8041 cggcctgccg gtcatgctgt cggagaccaa catcaagggg cgggtgtcgg atcgcctgag
     8101 ctggttgaag cacatggcgg aggagtgcgg caaggtcgtt gccgccggcg ccgacttccg
     8161 ggggttctgc tggttcccct tcgtggattc gaccgactgg tgctccctgg tgtgcagggc
     8221 cgagaagaag gtggacgccg tgggcatcta ttggctcgac gacgaccggt gggcccggca
     8281 cgcttcggag ctctcggact gcttcggcag gctggcgagg ggggagatgt cggtccaggc
     8341 gctgccgtcc taccggt
//
LOCUS       DASPJI010000473         8457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_492921, whole genome shotgun sequence.
ACCESSION   DASPJI010000473 DASPJI010000000
VERSION     DASPJI010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8457)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8457
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492921"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4746)
                     /locus_tag="VEL74_12940"
     CDS             complement(<1..4746)
                     /locus_tag="VEL74_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG63481.1"
                     /translation="MSEQDLDRLEGIAIVGMAGRFPCAADVEELWRNLAAGADCVSRF
                     SDEELEVPVPPEVLADPGYVKARGVLADPDLFDARFFDIAPRSAELMDPQHRLFLEHA
                     HQALEDAGIDPERYRGAIGVFGGVTLSSYFLFNLSSHPELLRSVGGYQLGLGTDRDYL
                     TTFVSYKLNLRGPSLDVQTACSTSLVATVLACQNLLSYTCDVALAGGVSVKLPQRSGY
                     MYQPGGLDSSDGYCRAFDAKADGSVYGGGVGVAVLKRLEDAVADGDTIHAVILGAAMN
                     NDGSAKVGFTAPGVDGQAEVITTAQALAGVDPETIGYVECHGSGTALGDPIEVTALTK
                     AFRAGNPDSERRGYCAIGSVKTNIGHCSAAAGIAGLLKTALALEHRQIPPSLHFEKPN
                     PAIDFENSPFYVAHRLLDWPANGSPRRAGVSSFGLGGTNAHVILQEAPEQEPSGPSRP
                     WQLLLLSAKTETALERATDNLAADLEAHPERSLADVAFTLQVGRRVFPHRRALVCRDV
                     EDARQALANRDPRRLLGAVREEGGRPVAFLLPGVGDHYPGMARGLYEAEPGFREALDR
                     CAELLRPRLGCDLREVIFPAVQEETAAGRPDLRRMLGRAPRGDDEAARRLHRTLHAQP
                     AVFAVEYALASLLGEWGIQPDALLGYSLGEYVAACLAGVLSLEDALLLVAERARLIDA
                     LPAGAMLAVPLPEAKLRPWLRSEVDLSALNAPGVCVASGPEEAVASLERRLQQEGVPS
                     RRLPTTHAFHSRSMEPVAPALERLARGVRLSAPRVPYVSNVTGTWITAAEATDPAYWV
                     RHLRQPVRFAEGLAALWKNPSRLLLEVGPGFGLSTLALQQTVADGGDEERLALPSIRN
                     EHDRQPDEAFLLGTLGRLWLAGADVDWAGFSARERRRRIRLPTYPFERQSYWIEPGRP
                     DEAGATPPRRRPERQEVVQGALSGDGFEQARAVAAGARLVLVEDDEIARRIARLRELE
                     ALGAEVVVVGASDAASLPAAPVVAERSGIVGGRHARPSLRNPYVPPSTDRESWLAGLF
                     QELLGIEPVGIHDSFFELGGHSLLGTSLLARIRDFFRLELPLRALFEAPTVAGLAQAL
                     EGEMDGVASAPEIRPVPRQGDRPLSYAQQRLWFLDRLMPGNPFYNLSGGMRLTGPLDL
                     GALRRSLREVGRRHETLRTRFAEVEGQPVQRTEPGFETGLPLVDLTGLPAGRRDEAVL
                     ATGVVSGSRPYDLTRPGLLRAALLRLDGEGTDHVLLFGMHHIVSDAWSMGILVREVAA
                     LYAAFSQGRPSPLPELPIQYADFAVWQRQWLTGEVLERQLRYWRERLTGVPGTELPSD
                     RPRPAVQTFRGGRWRTSFPQELAESLGNLARRHDASLFMVLLGAFKALLGRYSGEEDI
                     VIGSPIANRTHSGLESLIGFFVNTLVLRTDLSGDPGFPGLLERVKETSLGAQAHQDLP
                     FEQLVEELQPRRDLSRNPLFQVMFNLLNVPASRLVASELAMEPLPQPAETSLFDLQAY
                     VMETGAGLGISWEYSSDLFDAATIERFSAHYRTILEGIAAAPERRLSELPVLPETERR
                     QVLAGWNDTTLDYPGVCAHQLIEARARRSP"
     gene            complement(4743..>8457)
                     /locus_tag="VEL74_12945"
     CDS             complement(4743..>8457)
                     /locus_tag="VEL74_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63482.1"
                     /translation="VAERRDAPTAPVAQGEAAERAPGARDREFVPLRTPVEEWLAEIW
                     GEVLGTGVAIGAFDDFFDLGGHSLLATQVLARVRDSFGIELPVRTMFERPSLAGLATV
                     IEEAAWREGGDLPLRPERVKEAPLSYSQERLWFLDLLMPGTSIYNMPSPLRIEGPIAP
                     LRLERAFGELVRRHEALRTRFEERDGAPVQVVDPPRPWSLPVIDLSALPEAERRREAD
                     RWLLEEVAWPFDLTRGPLLRTLLLRLSLDEHVLLLTTHHIVSDGWSMGVLVREVITFY
                     RTFAAGEPPTLPALPIQYPDFARWQRQRLNGQALADHLAYWREQLAGSPPSLDLALDR
                     PRPAVQTYRGESVLVRMPAELTAGLKTIGLSERASLFMVLLAAFKLLLSRLSGQQDVV
                     VGSPVAGRERREFEGLIGFFLNTLALRTDLSGRPTFRELLRRVRTVALEAYSHQEVPF
                     EMLLADLQPERDLSRTPLFQVMFNMLNLPMEKVDVTGGVRIQLLGKHELESKFDLTVY
                     VTEEPGEELFLHLLYNADLFDRPRIEELARQYQEVLTQVAGRPEGPIHRVSLLTAEAS
                     ALLPDPVAPLGDEWRGAVHELFVEQARRQPRKPALTDLEGTWTYGDLEEASGRLAARL
                     AESGVGRRDRVAIYAHRSAPVVWAVLGTLRVGAAFVLLDPAYPAARLAEMVRLAAPKA
                     WLELAAAGPVPPELETLLAELGPACRLSLPAGGPEGARALLAGLPPEGVTVTVGPDDV
                     ALLAFTSGSTGTPKGIEGRHGPLSHFLPWQCERFGLGETDRYSMLSGLAHDPLQRDMF
                     TPLTTGGTVCIPQLEEIVTPGRLAAWMARQQITVAHLTPAMVQILTEPAAAGQAEGEQ
                     VEIPSLRYTLLVGDVLTRLDVDRIRSLAPRVTVVNLYGSTETQRAVGYHVVEDDGARV
                     ERSKQILPLGRGMQDVQLLVAAPSQPGEPPSLAGVGEVGEIWVRSPHLAKGYLGDEAL
                     TAERFRVNPFTGRPGDRVYRTGDLGRYLPDGQATFVGRADLQVKIRGFRIELGEIEAV
                     LGRLAGMREAVVIARQDGPDAGSRRLVAYVVLDPESAPAAGVEALRDALRERLPAYMV
                     PSAFVRLDKLPVTPNGKVDRKALPVPEGPGDGGVADFAEPEGDLEQAIAGVWREVLGL
                     EKVGVRHNFFDLGGHSLLLVRLHARLQQVLGRELTLVELFNYPNIRALAEHLGRKAGE
                     SSAASLEGAKDRAQKQIEAARRQKELAARRRGR"
BASE COUNT         1240 a         3054 c         3079 g         1084 t
ORIGIN      
        1 gggcgagcgc cgcgcccggg cttcgatgag ctggtgcgcg cagacgccgg gatagtccag
       61 ggtcgtgtcg ttccacccgg ccagcacctg ccgccgctca gtctccggca gcaccggcag
      121 ctcggacagg cgccgctccg gcgccgcggc gatcccttcg aggatcgtcc ggtagtgggc
      181 cgagaaacgc tcgatggtgg cggcgtcgaa caggtccgag ctgtactccc aggaaatacc
      241 gagccccgcc ccggtctcca tcacgtaggc ctggaggtcg aagagcgacg tctccgcggg
      301 ctgcggcagc ggctccatcg ccaactccga ggccacgagg cgtgaagccg gcacgttgag
      361 caggttgaac atcacctgga agagcggatt gcgcgacagg tcgcgccgcg gctgcagctc
      421 ctccaccaac tgctcgaacg gcaggtcctg gtgcgcctgg gcgccgaggc tcgtctcctt
      481 gacccgctcc aggagccccg gaaagcccgg atcacccgac agatcggtcc gcagcaccag
      541 ggtgttgacg aagaacccga tcaggctctc caggccgctg tgggtccggt tcgcgatggg
      601 cgagccgatc acgatgtctt cctcgccgct gtagcggccc agcagggcct tgaaggctcc
      661 caggaggacc atgaagaggg aggcgtcgtg ccgccgcgcc aggttcccca gggactcggc
      721 cagctcctgg gggaaggagg tccgccagcg gccaccgcgg aaggtctgga cggccggacg
      781 cggccggtcg gacggcagct ccgtacccgg cactccggtc agccgctcgc gccagtagcg
      841 cagttggcgc tccagcactt cgccggtcag ccactgccgc tgccagaccg cgaagtccgc
      901 gtactggatc ggcagctcgg gtagcggcga ggggcgcccc tgggagaacg ccgcgtagag
      961 ggccgccacc tcccgcacca ggatacccat ggaccaggcg tcggagacga tgtgatgcat
     1021 gccgaagagc aggacgtgat cggtgccctc gccgtcgaga cgcagcaggg cggcccgcaa
     1081 gaggccgggc cgggtgaggt cgtacggccg cgatccgctc accaccccgg tggcgagcac
     1141 cgcctcgtcc cgccgcccgg ccggcagccc ggtcaggtcg accagcggca ggccggtctc
     1201 gaagccgggc tcggtccgct gcaccggctg gccttccacc tcggcgaagc gggtgcgtag
     1261 cgtctcgtgg cgccgcccca cctcgcggag actccggcgc agcgcgccca ggtccagagg
     1321 cccggtgagg cgcattccgc ccgacaggtt gtagaagggg ttccccggca tgagccggtc
     1381 caggaaccag agccgctgct gcgcgtagga gagcggccgg tccccctggc gcggcaccgg
     1441 ccggatttcg ggtgcgctcg ccacgccgtc catctcccct tccagcgcct gcgccagccc
     1501 cgcgaccgtg ggcgcctcga acagcgcgcg gaggggcagc tccaggcgga agaagtcgcg
     1561 gatgcgcgcc aggagcgagg tgcccaggag cgagtggccg cccagctcga agaagctgtc
     1621 gtggatgccc accggctcga tgcccaggag ctcctggaag agcccggcga gccagctctc
     1681 gcggtcggtc gaggggggga cgtaggggtt ccgcaggctc ggccgggcat gacgcccgcc
     1741 cacgatccct gaacgctccg cgaccaccgg agcggccggc aggctcgcgg cgtccgaggc
     1801 gccgaccacc acgacctcgg cgcccagggc ttcgagctcc cgcagacggg cgatccggcg
     1861 ggcgatctcg tcgtcctcca ccaggaccag ccgtgccccg gccgccacgg cgcgggcctg
     1921 ctcgaagccg tccccggaga gcgccccctg gacgacttcc tggcgctcgg gccgccgccg
     1981 gggcggcgtg gcccccgcct cgtccggccg cccgggttcg atccagtagc tctggcgctc
     2041 gaaggggtag gtggggagcc ggatgcggcg gcggcgctca cgggcggaga agccggccca
     2101 gtccacgtcc gcgccggcga gccagagcct gcccagggtg ccgagcagga acgcctcgtc
     2161 cggctggcgg tcgtgctcgt tgcggatcga cgggagcgcg agccgctcct cgtctccccc
     2221 gtccgcgacc gtctgttgca gggccagcgt ggagaggccg aagccgggcc ccacctcgag
     2281 cagcaggcgc gaggggttct tccagagggc cgcgagcccc tcggcgaaac ggaccggctg
     2341 gcgcaggtga cgcacccagt acgccgggtc cgtggcctcg gcggcggtga tccaggtgcc
     2401 ggtgacgttg gagacgtagg gcacgcgggg ggccgagagc cgcacgccgc gcgccaggcg
     2461 ctccagcgcg ggcgccaccg gctccatgga gcgcgagtgg aaggcatggg tggtggggag
     2521 ccggcggctc ggcaccccct cctgctggag ccgccgctcg agggacgcga ccgcctcctc
     2581 gggaccggag gccacgcaga cacccggggc gttgagcgcg gagaggtcca cctcggacct
     2641 cagccagggc cgcagcttcg cctccggcag cggcaccgcg agcatggcgc cggcggggag
     2701 cgcgtcgatc agccgcgccc gctcggccac cagcaggaga gcgtcctcca gcgagaggac
     2761 cccggcgagg caggctgcca cgtactctcc caggctgtag ccgagcaggg cgtccggctg
     2821 gattccccac tcgcccagca ggctggcgag ggcatattcg accgcgaaga cggcgggctg
     2881 ggcatgcagg gtccggtgga ggcggcgagc cgcctcgtcg tcgccacgcg gagcgcggcc
     2941 caacatgcgc cgcaggtccg gccgccccgc cgcggtctct tcctgcacgg cagggaagat
     3001 cacctcccgc aggtcacagc cgagccgtgg gcggagcagc tcggcgcaac ggtcgagcgc
     3061 ctcgcggaag ccgggctccg cttcgtagag gccgcgggcc atccctggat agtggtcgcc
     3121 gacgccgggg aggaggaacg ccaccggccg gcccccctct tcgcgtacgg cgccgaggag
     3181 ccgccggggg tcccggttgg cgagcgcctg ccgggcgtcc tctacatcgc ggcagaccag
     3241 cgcccggcga tgcgggaaga cccggcgccc cacctgcagc gtgaacgcca cgtcggccag
     3301 gctgcgctcc ggatgcgctt ccaggtcggc cgccaggttg tcggtggcgc gctccagcgc
     3361 cgtctccgtc ttcgccgaca ggagcaggag ctgccagggg cgcgacgggc cggagggctc
     3421 ctgctccggc gcctcctgca ggatcacgtg cgcgttggtg ccgccgagcc cgaacgagct
     3481 gaccccggcg cggcgagggg aaccgttggc cggccagtcg aggagccggt gggcgacgta
     3541 gaacgggctg ttctcgaagt cgatggccgg gttgggcttc tcgaagtgca ggctgggggg
     3601 gatctgccgg tgctccagcg ccagcgcagt cttgagcagg ccggcaatgc ccgccgcggc
     3661 cgagcagtgg ccgatgttgg tcttgaccga gccgatggcg cagtagcccc ggcgctcgct
     3721 gtcggggttg ccggcgcgga acgccttggt cagcgcggtt acctcgatgg ggtcgcccaa
     3781 cgccgtgccg gagccgtggc actcgacata gccgatggtc tcagggtcga ctccggccag
     3841 cgcctgggcg gtggtgatca cctcggcctg gccgtccacc cccggcgcgg tgaagcccac
     3901 cttggccgag ccgtcgttgt tcatcgccgc gcccaggatg acggcgtgga tggtgtcgcc
     3961 gtccgccacc gcgtcctcca gccgcttgag caccgccacg ccgacgccgc caccatagac
     4021 cgagccgtcg gccttggcat cgaaggcgcg gcagtagccg tccgaggagt cgagcccccc
     4081 gggctggtac atgtagcccg acctctgggg tagcttgacc gagacgccgc cggccagggc
     4141 cacgtcacag gtataggaga ggaggttctg gcaagcgagg accgtggcca ccagcgacgt
     4201 cgagcaggcg gtctggacgt ccaggctcgg cccccgcagg ttcagcttgt aggaaacaaa
     4261 ggtcgtcagg taatcgcggt ctgttccgag cccgagctga tagccgccga cggagcgcag
     4321 cagctccggg tggctcgaga ggttgaacag gaagtagctg ctgagcgtca cgccgccgaa
     4381 gacgccgatg gcgccgcggt agcgttcggg atcgatgccc gcgtcctcca gggcctggtg
     4441 agcgtgctcc aggaagagcc ggtgctgcgg gtccatcagc tcggcgctac ggggcgcgat
     4501 gtcgaagaag cgggcgtcga agaggtccgg gtccgccagc acgccgcgcg ccttgacgta
     4561 gcccggatcg gccagcacct cgggcggtac cgggacctcc aactcctcgt cggagaagcg
     4621 cgacacgcag tcggcgccgg cggcgaggtt ccgccagagc tcctcgacgt ctgcggcgca
     4681 ggggaagcgc cccgccatgc caacgatggc gatgccttcg agccggtcga gatcctgctc
     4741 gctcaacggc ccctcctgcg ggcggccagc tccttctgcc ggcgggccgc ctcgatctgt
     4801 ttctgggccc ggtccttggc cccttccaga gacgcggccg aggattcgcc ggccttccgg
     4861 cccaggtgct cggccagggc cctgatgttc ggatagttga acagctctac cagcgtcagc
     4921 tcccgcccga ggacctgctg cagccgggcg tgcagccgga cgaggagcag cgagtgcccc
     4981 ccgaggtcga agaagttgtg ccggaccccg accttctcca agccaagaac ctcccgccag
     5041 acaccggcga tggcctgctc caggtccccc tcaggctccg cgaagtcggc cacgccgccg
     5101 tcacccggcc cctccgggac cggcagggcc ttgcggtcca ccttgccgtt tggcgtgacc
     5161 gggagcttgt cgagccggac gaacgccgac ggcaccatgt aggcggggag ccgctcccgt
     5221 agcgcgtccc gcaacgcctc tacccccgcg gcgggggcgc tttcgggatc cagaaccaca
     5281 taagccacca ggcggcggga gccggcgtcc ggcccatcct gccgggcgat gaccacggcc
     5341 tcgcgcatgc cggcgaggcg gccgagcacg gcttcgatct cgcccagctc gatgcggaag
     5401 ccgcggatct tgacctgcag atcggcccgg ccgacgaagg tcgcctggcc gtcgggcaga
     5461 tagcggccga gatcgccggt gcggtagacc cggtcgccgg ggcggccggt gaacgggttg
     5521 acccggaacc gctcggcggt gagcgcttcg tcgcccaggt accccttggc gaggtggggg
     5581 ctccgcaccc agatctcccc cacctctccg accccggcca gggacggagg ctcaccgggc
     5641 tgggatgggg cggccaccag gagctggacg tcctgcatgc cgcgccccag ggggaggatc
     5701 tgcttggacc tctccacgcg ggcaccgtca tcctccacca cgtggtagcc cacggcgcgc
     5761 tgggtctccg tgctgccgta caggttgacc acggtgaccc gcggcgcgag cgagcggatg
     5821 cggtccacgt cgagccgggt gaggacgtcg cccaccagga gcgtgtagcg caacgagggg
     5881 atctccacct gctcaccttc ggcctgtccc gcggcggcgg gctcggtgag gatctgcacc
     5941 atggccggcg tcagatgggc gacggtgatc tgctggcgtg ccatccatgc cgccaggcgg
     6001 cccggggtga cgatctcctc gagctgcggg atgcacacgg tgccaccggt ggtgagcggc
     6061 gtgaacatgt cgcgctgcaa ggggtcgtgc gccagcccgg agagcatgct gtagcggtcc
     6121 gtctcgccga ggccgaagcg ctcgcactgc caggggagga agtgggagag cggcccatgc
     6181 cgcccctcga tccccttggg agtcccggtc gagcccgagg tgaaggccag cagggccaca
     6241 tcgtccggcc cgaccgtgac ggtgacgccc tccggcggca ggccggcgag cagggcgcgc
     6301 gcgccctcgg gcccaccggc cggcagcgac agacggcagg cggggccgag ctccgccagc
     6361 aaggtctcca gctccggcgg gaccggcccg gcggcggcga gctcgagcca ggccttcggc
     6421 gcggcgagcc gcaccatctc cgccaagcgc gccgccggat aggccggatc gagcagcacg
     6481 aaggccgctc ccacgcggag ggtgcccagc accgcccaga ccaccggcgc gcttcggtgg
     6541 gcgtagatgg ccacccggtc cctgcggccg acgccgcttt cggcgagccg cgcggcgaga
     6601 cggccggaag cctcctccag gtcgccgtag gtccaggtcc cctccaggtc ggtcagggcg
     6661 ggcttccgcg gctggcggcg ggcctgctcg acgaacagct cgtgcaccgc gccgcgccac
     6721 tcgtcgccca gcggcgccac gggatcgggc agcagcgccg acgcctccgc cgtgagcagc
     6781 gacacccgat ggatcgggcc ttcggggcgc ccggcgacct gggtgagcac ctcctggtac
     6841 tgccgtgcca gctcctcgat gcgcggccgg tcgaagaggt cggcgttgta gaggaggtgg
     6901 aggaacagct cctcgcccgg ctcctccgtg acgtagacgg tgaggtcgaa cttggactcg
     6961 agctcgtgct tgcccaggag ctggatccgc actccccccg tcacgtccac cttctccatc
     7021 gggaggttca gcatgttgaa catcacctgg aagagcggcg tccgggacag gtcgcgctcg
     7081 ggctggagat ccgccaacag catctcgaac gggacctcct ggtgggagta ggcctccagg
     7141 gcgaccgtgc ggacgcggcg cagcagctcg cggaacgtgg gccgtcccga gaggtcggtg
     7201 cgcagcgcca gagtgttgag gaagaacccg atgagcccct cgaattcccg ccgctcgcgt
     7261 ccggcgaccg gcgagcccac gaccacgtcc tgctggccac tgagccgcga gagcagcagc
     7321 ttgaaggcgg cgagcaggac catgaacaga gatgcccgct cggacaggcc gatcgtcttg
     7381 aggcccgccg tcagctccgc gggcatcctg accaggaccg actcgccgcg ataggtctgg
     7441 acggccggcc tggggcggtc cagtgcgagg tccaggctcg gcgggctgcc ggcgagctgc
     7501 tcgcgccagt aggcgaggtg atccgccagg gcctgcccgt tcaggcgctg gcgctgccag
     7561 cgggcgaaat cgggatactg gatcggcagc gccggcagcg tcgggggctc acccgccgcg
     7621 aaggtccggt agaaggtgat cacctcacgg accagcactc ccatcgacca gccgtccgag
     7681 acgatgtggt gggtcgtcag gagcagcacg tgctcgtcga ggctcagccg cagcagcagg
     7741 gtgcgcagca gcggcccacg cgtgaggtcg aagggccagg ccacctcctc caggagccag
     7801 cggtcggcct cgcggcgccg ttcggcctcc ggcagcgccg acaggtcgat cacgggcagg
     7861 ctccacggcc gcggcggatc gacgacctgt accggcgcgc cgtcccgctc ctcgaaccgc
     7921 gtgcgcagcg cctcgtgccg gcgcaccagc tcgccaaagg cgcgctccag ccgcaggggc
     7981 gcgatcgggc cctcgatgcg gaggggcgag ggcatgttgt agatcgaggt ccccggcatc
     8041 agcaggtcga ggaaccagag ccgctcctgg gagtacgaga gcggagcctc cttcacccgc
     8101 tccggacgga gcggcaggtc cccgccttcg cgccaggccg cctcctcgat cacggtagcc
     8161 agccccgcca gcgacggacg ctcgaacatc gtccgcaccg gcagctcgat gccgaaggaa
     8221 tcgcgcaccc gcgccagcac ctgggtcgcc aggagggagt gcccgccgag gtcgaagaag
     8281 tcgtcgaagg cgccgatcgc cacgccggtc cccagcacct cgccccagat ctccgccagc
     8341 cactcctcca ccggggtacg gagcggcacg aactcccggt cccgtgcccc cggggccctc
     8401 tctgccgcct cgccctgcgc gaccggcgcg gtgggcgcgt cccgcctctc cgcgacc
//
LOCUS       DASPJI010000474         5814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_837359, whole genome shotgun sequence.
ACCESSION   DASPJI010000474 DASPJI010000000
VERSION     DASPJI010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837359"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VEL74_12950"
     CDS             complement(<1..315)
                     /locus_tag="VEL74_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63483.1"
                     /translation="MKRSRLLRCCLGLMATALCATALGLSPRLAEAACFCPMAGTWSS
                     APGIAYSTAGCPGSACLDNARANAEASCDNGVCAWGSSSPTSCVIIGGQYRSTCSVQY
                     KCF"
     gene            complement(1114..2904)
                     /locus_tag="VEL74_12955"
     CDS             complement(1114..2904)
                     /locus_tag="VEL74_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63484.1"
                     /translation="MPQARVQHFPDQDPPLFSFRERERRRSFRTPLFERADGDGAVRE
                     LTWGERVEIVERGDERSRVRTAAGDEGFAETRFLVETLHAGNRGMERGLPLLKAPQGD
                     EKHCDLLWGDPVQVLERGEGRWKVRARGRNGFVGPDDLMEEGLLELYFIDVGQGDGVL
                     VRTPDGRHLLIDGGYRRVSQSHGKSAADFVDWKFFHDYGLLHVELDAMIASHCDADHH
                     GGLWDMISASPRDRQELDTEATEVRAFFHAGVSWWKREDGERYLGPEKGGHLVQLLGG
                     FAGARAAVQDGADPRLQGQWGQLIERVVELVEPEGFRRLGVERGGARQFMPGYEDGDF
                     TIEVLAPVTGTVDGAPSLPDLGSASQNTNGHSLLLGLRYGRAHLLLTGDLNKRSMHHL
                     LEAYSGREEIFACDVAKGCHHGSDDVSLRFLEAMHAAATIISSGDNEGHAHPRPAIVA
                     ASALTGRREVDEEEDELITPLVYATEIERSVRLGRLERIQATSYPHGEESLEVQVYAV
                     DPDRLQADFRNDARLKRAVKTRLFYGETTPGAFRATRADRSVRGAYVVSGIVYGLVNV
                     RTDGRRILCATRNEGKDAWTIRTFEARSGA"
     gene            3053..4477
                     /locus_tag="VEL74_12960"
     CDS             3053..4477
                     /locus_tag="VEL74_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63485.1"
                     /translation="MSGECLRRYRRRAGVFAVLVVALALAGAPALSAQVTRGGGLPAP
                     LPLFPPDNWWNVDISAAPVDPNSANFINFIGATRKLHPDFGGLVDPDDPSNPATYGMT
                     FITVPGSQPLEPVTWVEYGDQSDDGAPGRPAGYPIPVEARTQPGWIEGGWPANQTGGD
                     RHMLIVDRDNRILYELYHTRWNPTLNRWEAGSGAIFRLDSNERRPDTWTSADAAGLAI
                     LPGLIRYDEVFGTEPIRHALRFTVQSTNGYVFPASHRAGSTSGALPMGARLRLKASKD
                     ISGQPPEIQKIFQAMKTYGLIVADNGSNMYIQGTHDTRWNNDVLNPAFRSLTAGDFEV
                     IELGWQPPVATSLSDLSFYTLSPCRVLDTRQPFGPYGGPAVPPSSERVVVVTGRCGIP
                     AGAKAISVNVTVVNPPGTGHLAFFPGNATPQTTSMINFRPGLTRANNAILMLASSGSG
                     TLGVESVTTGGSTHVLIDVNGYFQ"
     gene            4514..5758
                     /locus_tag="VEL74_12965"
     CDS             4514..5758
                     /locus_tag="VEL74_12965"
                     /EC_number="2.5.1.76"
                     /inference="COORDINATES: protein motif:HMM:TIGR03844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteate synthase"
                     /protein_id="HYG63486.1"
                     /translation="MGAFTLVCLSCGREHEEFRMRCQDGCDALLRARYAARSFRPAAA
                     ESLFKFSDWLPCQGSVETGIGPVVYECGALGRKLGLERLYAAFNGYWPERGAYNSTGT
                     FKDLEALPTVLAFREHGKRRILLSSAGNTARAFAHAVQVVDGFEVILVVPEGMLHRLW
                     LPGGDRTGQVKLVAVQGSQDYYEAITLGDEIARRYGLDVEGGARNVARRDGMGTVMLE
                     AARLLGRLPDHYVQAVGSGTGGIAVYEAALRLRQDSRFEDQPLPRLHLGQNAPFLPIH
                     HAWTNGQRIDPERCKGVSEQVYASVLANRNPPFGMAGGVAEALRESRGATYAVETPEA
                     EEAARSFERLEGIDLEPAAAVAVAVLRQAVEAGAIRRDELVLLNLTGGGLERIRRDFD
                     CVPLEPVLRAAPGSVEEVASIL"
BASE COUNT          907 a         2002 c         1973 g          932 t
ORIGIN      
        1 aaagcacttg tactgcacgc tgcaggtgga cctgtactgt ccgccgatga tgacgcaact
       61 ggtcggggag ctgctgcccc aggcgcagac gccattgtcg cagctcgcct cggcgttggc
      121 ccgcgcattg tccaggcagg cgctgccagg acacccggcc gtgctgtagg cgatgccggg
      181 agccgagctc caggtacccg ccatgggaca gaagcaggcg gcttcggcca ggcgcggcga
      241 aagcccgagc gccgtggcgc atagagccgt cgccatcaga ccgaggcagc acctcaagag
      301 cctagatctc ttcatcagga acctccttgt cggcaacgtg cctcgcaaca tgcgaggcac
      361 ctatcacgag cctcgtgaac cggtgaaatc acgctccacc agggcacaag gccggataac
      421 cacctggcct gcgctttctc ctacggctcg gcattgagcc ctcttaatta ggtatacgga
      481 tgagaaggcg atcacgtttc acccccctgc ccagtgctgg aggcgaagga ctgtccctcc
      541 gcctcccttg ttgagggcgc gagcgttccg gggcgcggcc ttgggcacgg cggcaccccc
      601 aaaaaattca gttgtcaagg aacctggcaa gcaggcaaag acccgctcca tggcgggcgc
      661 atgatacacc atgattgacg ttttagccta gattgcggac aatcaatccg cgacaggcac
      721 tcagccgttg agtgctgctc cggctctttg acaattgatc gacatgtcgc ctgggcgcgg
      781 tgccgggacg gggcgagccg cgtccgggga catggtttgg atcacaatgg aaggtggcga
      841 gatcattcgg gaggccggga ggatcactcc ctccgacggg agaatgatcc gggaagggcg
      901 gagagtgatc ctccccgcgc tccggaacac aaaccggggt ccggaagggt ccggggacat
      961 ccgggaatgc tccctccccg cggggagagt gatccggcac gcggggagga tgattcgctc
     1021 gctccggatt gtgatcccgg aaggccgatt tgtgttccgg gagagcctgt ttgtgttccc
     1081 ggagtgttcc gtagtgatcc ggtgtgttcc ggatcaggcc cccgagcgcg cctcgaaggt
     1141 gcggatggtc caggcgtcct tgccctcgtt gcgggtggcg cagaggatgc ggcggccgtc
     1201 tgtccggacg ttgacgaggc cgtagacgat gccggacacc acgtaggccc cgcgcacgga
     1261 gcggtccgcc cgcgtcgccc ggaaggcacc gggtgtggtc tcgccataga agaggcgagt
     1321 cttcacggcc cgcttgagcc gggcatcgtt ccggaagtcc gcctgcagtc tgtccggatc
     1381 gacggcgtag acctggacct cgaggctctc ctctccgtgg ggataggagg tcgcctggat
     1441 gcgctccagc ctgccgaggc ggacgctgcg ctcgatctcg gtggcgtaca cgagaggggt
     1501 gatcagctcg tcctcctcct cgtcgacctc ccggcggccg gtgagggcgc tggcggctac
     1561 gatggcggga cgggggtgcg cgtgcccctc gttgtcgccc gaggagatga tggtggcggc
     1621 ggcgtgcatc gcctccagga agcggagcga gacgtcgtcg ctgccgtggt ggcagccctt
     1681 ggccacgtcg caggcgaaga tctcctcgcg ccccgagtag gcctccagga ggtggtgcat
     1741 gctccgcttg ttcaggtcgc cggtcaggag gaggtgcgcc cggccgtagc gcagtccgag
     1801 caggaggctg tggccgttgg tgttctggct ggccgagccg aggtcgggca gagagggggc
     1861 gccgtcgacg gtgccggtga ccggggcgag gacctcgatg gtgaagtcgc cgtcctcgta
     1921 gccgggcatg aactgccggg cccctccccg ctcgacgccg aggcgccgga agccctcggg
     1981 ctccaccagc tcgaccaccc tctcgatcaa ctgcccccac tgtccctgga gccgcgggtc
     2041 ggcgccgtcc tggaccgcgg cccgcgcgcc ggcgaagccg ccgagcagtt gcacgaggtg
     2101 accgcccttc tccggcccga ggtagcgctc cccgtcctcc cgcttccacc aggagacgcc
     2161 ggcatggaag aaggcgcgga cctccgtggc ttcggtatcc agctcctgcc ggtcccgggg
     2221 gctggcgctg atcatgtccc agaggccgcc gtggtgatcg gcgtcgcaat gggaggcgat
     2281 catcgcgtcg agctcgacgt ggaggagccc gtaatcgtga aagaacttcc agtcgacgaa
     2341 gtcggcggcg ctcttgccgt ggctctggct cacccgccga tagccgccgt cgatgagcag
     2401 gtggcggccg tcgggggtgc ggaccagcac gccgtccccc tgcccgacgt cgatgaagta
     2461 cagctcgagg aggccctcct ccatgaggtc gtcggggccc acgaagccgt tcctcccgcg
     2521 cgcccggacc ttccagcgcc cctccccgcg ctcgagcacc tggaccgggt caccccacag
     2581 gaggtcgcag tgcttctcgt ccccctgggg cgccttgagg aggggaaggc cgcgctccat
     2641 gccgcggttg ccggcgtgga gggtctcgac caggaaccgt gtctcggcga agccctcgtc
     2701 cccggcggcg gtccggaccc gcgagcgctc gtccccgcgc tccacgatct cgacccgctc
     2761 cccccaggtc agctcgcgga ctgcgccgtc accgtccgcc cgctcgaaga gaggcgtccg
     2821 gaagctccgg cgccgctcgc gctccctgaa gctgaagagg ggtgggtcct ggtcaggaaa
     2881 gtgttgcacg cgggcttgag gcatgattcg agccctcccg ggtcggagcc gttccttcgt
     2941 accgctcgga tacagactcc accagcatac acctgttggc ggagtgctga aaaagagtca
     3001 cctggactct tgcgcattct tcgatagact tggggaacca agaggagatc ccatgtctgg
     3061 agaatgcctt cggaggtatc gccgtcgtgc cggtgtgttc gcagtcctgg tggtggctct
     3121 ggctctagcc ggcgccccgg ccctctccgc ccaggtgacg cgcggcggcg ggttgcctgc
     3181 tcccctcccc ctgttcccgc cggacaactg gtggaacgtg gatatcagcg ccgcgccggt
     3241 cgaccccaac agcgccaact tcatcaactt catcggcgcc acccgaaagc tccaccccga
     3301 tttcggcggc ttggtcgatc ccgatgaccc gtcaaacccc gcgacctacg gcatgacctt
     3361 catcaccgtg ccgggcagcc agccgctgga gccggtcacc tgggtggagt acggtgacca
     3421 gagcgacgac ggcgctccgg gacggccggc gggctatccg atcccggtcg aggcgcggac
     3481 tcagccagga tggatcgagg ggggctggcc cgcgaaccag accggcggcg accgccacat
     3541 gctcatcgtc gaccgggaca accgcatcct ctacgagctc taccacaccc ggtggaaccc
     3601 caccctgaac cgctgggagg cgggctccgg cgccatcttc aggctggact ccaacgagcg
     3661 ccgcccggac acctggacca gcgccgacgc cgccgggctc gccatcctcc ccggcctcat
     3721 ccgttatgac gaggtcttcg ggacggagcc gatccgccac gccctccgct tcaccgtgca
     3781 gagcaccaac gggtatgtgt tcccggcctc tcaccgggca gggagcactt cgggcgccct
     3841 ccccatgggc gcgcgcctcc gcctcaaggc gagcaaggac atctcgggcc agccccccga
     3901 gatccagaag atcttccagg cgatgaagac ctacggcctg atcgtcgcgg acaacggctc
     3961 gaacatgtac atccagggga cccacgacac ccgctggaac aacgatgtcc tgaacccggc
     4021 gttccggtcc ctcacggccg gcgacttcga ggtgatcgag ctcggctggc agccgccggt
     4081 ggccacctcg ctcagcgatc tctccttcta caccctctcc ccctgccggg tgctcgacac
     4141 ccgccagccg ttcggcccgt acggcggacc tgcggtgccg ccgtcctccg agcgggtggt
     4201 ggtggtcacc ggccgttgcg gcatcccggc cggggcgaag gcgatctcgg tcaacgtcac
     4261 cgtggtcaac ccgccgggga ccggacacct ggccttcttc ccgggcaacg ccacgcccca
     4321 gacgaccagc atgatcaatt tccggcccgg cctcacgcgg gccaacaacg ccatcctcat
     4381 gctcgcttct tccggctccg gcaccttggg agttgagagc gtgaccaccg gtggctcgac
     4441 gcatgtgctg atcgacgtca acggttactt tcagtagcag ccctggatcc cgctctccga
     4501 aggaaggaga agcatgggcg cctttacact cgtctgcctg agctgcggcc gtgagcacga
     4561 ggaattccgg atgcgctgcc aggacgggtg cgacgccctg ctgcgggcgc gctacgcggc
     4621 gaggagcttc cgtccggcag ccgccgagag cctgttcaag ttctccgatt ggctcccctg
     4681 ccagggcagc gtcgagacgg ggatcgggcc ggtggtctac gaatgcggtg ccctgggccg
     4741 gaagctgggc ctcgaacgcc tctacgccgc gttcaacggc tactggccgg agcggggcgc
     4801 gtacaactcc accggcacct tcaaggacct ggaggccctg cccaccgtcc tcgccttccg
     4861 ggagcatggc aagcggcgca tcctcctctc ttcggccggc aacacggccc gggccttcgc
     4921 ccacgccgtc caggtggtcg acggcttcga ggtgatcctg gtggtgcccg aggggatgct
     4981 ccaccggttg tggctgcccg gcggggaccg caccggccag gtgaagctgg tcgccgtcca
     5041 ggggagccag gattactacg aagcgatcac cctgggggac gagatcgccc ggcgttatgg
     5101 tctcgacgtc gaggggggag cgcggaacgt ggcccgccgg gatggcatgg ggacggtgat
     5161 gctggaggcg gcacggctgc tgggaaggct gccggaccat tatgtccagg ccgtcggcag
     5221 cggcaccggc gggatcgccg tgtacgaagc cgccctgcgg ctgcgccagg actcccgttt
     5281 cgaggatcag ccccttcccc ggctccacct cggacagaac gcccccttct tgccgatcca
     5341 ccacgcctgg acgaacggcc agaggatcga tccggagcgc tgcaaggggg tctccgagca
     5401 ggtgtacgcc agcgtcctgg cgaaccgcaa tccgcccttc ggcatggccg gcggcgtggc
     5461 cgaggccctt cgtgagagcc gcggcgccac ctacgcggta gagacgccgg aggccgagga
     5521 ggccgcccgg agcttcgagc ggctcgaggg gatcgacctc gagccggccg cggccgtggc
     5581 ggtcgccgtc ctccggcaag cggtggaggc cggagcgatc cgccgcgacg agctggtgct
     5641 gctcaacctg accggcggcg ggctcgagcg catccgccgg gatttcgatt gcgtgccgct
     5701 tgaaccggtg ctccgcgccg cgcccggcag cgtggaggag gtggcctcca tcctctagag
     5761 tgatagcctt gccctggaat ggatggggaa cggctcgacc tccgtgtgat cgcc
//
LOCUS       DASPJI010000475         8733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_199319, whole genome shotgun sequence.
ACCESSION   DASPJI010000475 DASPJI010000000
VERSION     DASPJI010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8733)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8733
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199319"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2070)
                     /locus_tag="VEL74_12970"
     CDS             complement(<1..2070)
                     /locus_tag="VEL74_12970"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYG63487.1"
                     /translation="MNRLSRVSIRHPLRTLAAAALVALAAAPGIARLELRTEGQALVP
                     SRAPAVVQDREVRRLFGVRDSMVVVVRTEHPDGIFNPGTLRHVRDLTAALGRLEGLGS
                     ADVTSLATETGFHFRPGTLERSPLLEPLPDTPEALTALRRDVHRIGLYDGLLVSADGT
                     GTAVVLGVPPGVDCQAFYREVQRVASAPSPARDRVEVLGAPVAETLLGIHILSDLGVP
                     SRWLGGAMGEERSGPGLIPLALGVMGMVFLLGFRRPAAALLPLLKLGVCLLVVFGLMG
                     WLSVPVYLTTAVLPVILIAVGMTGEVHLFRRFAVLYAERPDDASAELARVALEQVERP
                     ILQASAIAAIGFLSFSVTTLAPVRAFGLFAAAGVGLCLLWSLTASPALLALLPPAWMA
                     RRASGPREEKEKPFGRLAGLAAHRRVILAGVAILGLLSLDGLRRLEVQDSWVDGFAPG
                     SVFAREMRRFDDQYHGAHTLLIAVTGQPRRLAGEVGGASVGDRSLVLPLRAAGGVPAA
                     AFEGGFLGLESREALPAALPAAGPRQWSSWVESSIAEGDRLVLSWPLTGGSPQFWLQP
                     SPEAPVDYEVRLEPLSVPANLRRIRDLEGFLAELPGVGGVLGPAKFLETASFLSQPDA
                     AGSRRLPETPDEALVLWSNAEVARGPERLRRLVDEERSQGLVTVFLKSSNYAATERLM
                     REVREYER"
     gene            complement(2131..2832)
                     /locus_tag="VEL74_12975"
     CDS             complement(2131..2832)
                     /locus_tag="VEL74_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63488.1"
                     /translation="MKTTRSGWTRTIFMSLGLALVVGIGHTAPPPPPQCFEVDNGSGT
                     VTLPPANCEYLGPNDVHKIIAGLPVGTEIILAPIHRNFICRTGGACGSSGGSLGGERE
                     VFSSDIVFQVTGTGALSGWSRTLTIPLQVETHTAPRTPGAPVQSFRTDMYRIQGGITN
                     DPDFGSFTIRGGTANGYPSPGHTTLTRQLNRTFHVDSSFDVTVAIDFVGAGPRLGNYR
                     GRTVRKVTMKAFGEQ"
     gene            complement(3043..3441)
                     /locus_tag="VEL74_12980"
     CDS             complement(3043..3441)
                     /locus_tag="VEL74_12980"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004117178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYG63489.1"
                     /translation="MPDILQPAGWAAPGGYVNGMATSGRLVFVAGQIGWNPETCAFET
                     DDFVAQTARALENVAAVVREAGAEPAHLVRVTWYITDKAAYLANQKEIGRAWRDVMGR
                     HYPAMTLVVVAALLEDRAKVEIEATAVVPE"
     gene            complement(3443..5098)
                     /locus_tag="VEL74_12985"
     CDS             complement(3443..5098)
                     /locus_tag="VEL74_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYG63490.1"
                     /translation="MAESTAPEPSAHVDTFCREALPPRELWPEMIYTGVPELRYPPRL
                     NCAVELLDELVAAGCGERTVFHFPGGRWTYQQLLETANRIAHVLVDDLGVVPGNRVLL
                     RGPNNPMMAACWFAVLKAGGVVVATMPLLRVRELTYIADKAKIRLALTDSRIAGDCEQ
                     AMATTGAGFPREGARVIPFHSAGPSSLESLMQGKPTTFDNCDTAADDTALIAFTSGTT
                     GQGKGTMHFHRDVLAVCDCFPPYILKPEADDIYCGSPPLAFTFGLGGLLLFPMRAGAS
                     TILLEQASPPQLLQGIQDHRATVCFTAPTAYRAMAGMAKGFDISSLRKCVSAGETLPK
                     STFELWEETTGIKIIDGIGSTEMLHIFISSSGDDIRPGSTGRAVPGYQARVVDEGFHE
                     VPPGTIGRLAVKGPTGCRYLDDLERQRGYVQNGWNLTGDSYVMDEDGYFWYQARTDDM
                     IISAGHNISGPEVEDVLLEHPAVAECGVVGVPDEERGQIVKAFVVLRQGFAAAPELVK
                     ELQDHVKAEIAPYKYPRSVEFVDSLPRTLTGKLQRFRLREREE"
     gene            complement(5091..6311)
                     /locus_tag="VEL74_12990"
     CDS             complement(5091..6311)
                     /locus_tag="VEL74_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYG63491.1"
                     /translation="MPDVTFLSWPFFDDAHRALVPELDAWCEREIAPLEGWEDEALDA
                     TCREIVHRLGQGGWLRYAVPRGAEQGGTGRLDVRALCLIRETLGRHSGIADFAFALQG
                     LGSGPLSLFGSEEQRRTWLPRVAAGRAISAFALSETDSGSDVSSMTTTARRDGDGWVI
                     EGEKTWISNAGLADFYVVFCRLPEEGEKAFAALLVEADNPGLKVTRRIDVLAPHPLGT
                     LSFEDCRVPEGALVGEPGKGLKVALGTLDVFRSTVGAAALGFARRALDEALAHAVRRK
                     AFGKPLAEFQLTQAKIADMATAVDASALLVYRAAWTRDTGAARVTREAAMAKLFATEA
                     AQRVIDEAVQLLGGRGVMSGEPVEKLYREVRALRIYEGTSEIQRLVIAGQTFAAAPPS
                     QPAPASTGGSTHHG"
     gene            complement(6311..7144)
                     /locus_tag="VEL74_12995"
     CDS             complement(6311..7144)
                     /locus_tag="VEL74_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HYG63492.1"
                     /translation="MNNRISLEEIQPRHFGWSVEDRVATVTISRPERKNPLTFESYRE
                     LSDTFHRLRHVEEVKAVVLTGAGENFSSGGDVHEIIGPLVRMKEEGRMDRLLEFTRLT
                     GELVRTMRHCPQPIIAAVDGICAGAGAILAMASDMRLGTPRSRVAFLFTRVGLSGADM
                     GACAILPRIIGQGRASELLYTGRFMEAAEAERWGFFNRLVEPGDLLAEAIALARSIAH
                     GPTFAHAMTKNQLHQEWSMGVDEAIEAEAQAQAICMQTEDFARAFRAFAAKEKPVFEG
                     N"
     gene            7247..8269
                     /locus_tag="VEL74_13000"
     CDS             7247..8269
                     /locus_tag="VEL74_13000"
                     /inference="COORDINATES: protein motif:HMM:NF025394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase C-terminal
                     domain-containing protein"
                     /protein_id="HYG63493.1"
                     /translation="MSALYPDPLLSSSLYGTGRLDALIVEMVAPFWREVRDQAGDAYL
                     WMLRYGRRGEHLKLRLHGPEDLRPLLAPQLEAAAESYFAARERRPRPAEPVKRRPAPP
                     LDEEDHGRDDHPDDSFLWTRSRRVPMVLGAAPLLHDDRYAALFTTCLGRACELILDWL
                     ELEDGGTVSFRSRQGTLMMLLSTGLAAAFPDEEERVRYLTYHRDWLVRSPVLYTRGRI
                     EKARAILDRFDQEAERMGSAAQVSLAEVVYADGDAEDDEPAEAAWRRSVAELRAYLEP
                     LQGDPVYDLDPFVEGPLHPCLFKLFHCVANQIGLTALNEALTWHLLVHAADEESGEFR
                     LVPAGV"
     gene            8271..>8733
                     /locus_tag="VEL74_13005"
     CDS             8271..>8733
                     /locus_tag="VEL74_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63494.1"
                     /translation="MDDKSFNFEKDYDWTALVAGMGGGMGGGMGGGAQGWLDEYSRVG
                     SEIFDDIRWGLGLLRRREMEEGLARLDRARSRWEPLRDSSPAIYHVLGRYYHGALAYW
                     HYCVDDFPRADETLRQALDAVRSAVEAEPFLLPFAAVSMDIPLKQVQIARAL"
BASE COUNT         1347 a         3054 c         3051 g         1281 t
ORIGIN      
        1 ccgctcgtac tcccgcacct cccgcatcag ccgctcggtg gcggcgtagt tggagctctt
       61 caggaagacg gtcacgaggc cctgggagcg ctcctcgtcc accagccggc ggagacgctc
      121 gggcccccgc gccacctcgg cgttgctcca caggaccagg gcctcgtccg gagtctcggg
      181 gagccgccgc gaccccgccg cgtcgggctg gctcaggaag ctcgccgtct ccaggaactt
      241 cgccgggccc agcaccccgc ccactcccgg cagctctgcc aggaagcctt ccagatcccg
      301 gatccgccgg aggttggccg gcacggatag cggctccagc cgcacctcgt agtcgacagg
      361 agcctcgggg ctcggctgga gccaaaactg cggcgatcct ccggtcagcg gccaggagag
      421 gaccaggcgg tctccctcgg cgatggagct ctccacccag gaggaccact ggcgcggccc
      481 tgctgccgga agagccgccg gcagggcctc gcggctctcg agaccgagga agccgccctc
      541 gaacgccgcc gcagggaccc cacccgccgc acggagcggc aggaccaggc tccggtcccc
      601 cacgctcgct cccccgacct cgcccgcgag gcggcgcggc tggcccgtca cggcgatcag
      661 caacgtgtgg gcaccatgat actgatcgtc gaaccggcgc atctcccggg cgaagacgct
      721 ccctggcgcg aagccgtcca cccaactgtc ctgcacctcg aggcggcgca agccatccag
      781 ggacagcagg ccgaggatcg ctacgccggc caggatcacc cggcggtggg cggccagccc
      841 tgccaggcgg ccgaagggtt tctctttctc ctctctcggg ccggacgcgc ggcgggccat
      901 ccaggccggg ggtaggaggg cgaggagcgc ggggctcgcg gtgagcgacc agagaaggca
      961 gagcccgact ccggccgccg cgaacagccc gaaagcgcgg acgggggcga gcgtcgtgac
     1021 cgagaaggag aggaatccga tggccgcgat cgccgaagct tgcaggatcg gccgctccac
     1081 ctgctcgagg gccacgcggg ccagctcggc gctggcgtcg tcgggccgct cagcgtagag
     1141 gaccgcgaag cgccggaaga ggtggacctc gcccgtcatc ccaaccgcga tgaggatcac
     1201 cggcaggacc gctgtggtga gatagaccgg aacgctgagc catcccatca gaccgaagac
     1261 caccagcagg cagaccccca gcttgaggag aggcagcagg gcggcggccg ggcgcctgaa
     1321 tcccaggagg aagaccattc ccatcacccc cagtgccagg gggatcaggc cgggcccact
     1381 cctctcctct cccatcgccc cgccgagcca tctcgacggc accccgagat cggacaggat
     1441 gtgaatcccc aacagggtct cggccaccgg cgcgcccagc acctcgacgc ggtcacgcgc
     1501 ggggctcgga gccgaggcga ccctctggac ctcacggtag aaggcttggc agtccactcc
     1561 tggaggcacg cccagaacca ccgccgtgcc agtgccgtcg gcggagacga gcaggccgtc
     1621 gtagagaccg atccggtgga catcccggcg cagcgcggtg agtgcctcgg gggtgtccgg
     1681 cagcggctcg agcagcgggc tccgctccag cgtccccggc cggaaatgga aaccggtctc
     1741 ggtcgccagg ctggtgacgt ccgccgagcc gagcccttcg agccggccca gggccgcggt
     1801 caggtcgcgg acgtggcgta gcgtcccggg attgaagatc ccgtcgggat gctccgtccg
     1861 cacgacgacc accatggaat cgcggacacc gaagagccgg cggacctcgc ggtcctggac
     1921 cacggccggg gcgcgcgaag gcaccagcgc ctgcccctcg gtccgcaact cgaggcgtgc
     1981 gatgcccggc gcggcggcga gcgccaccag ggcggcggcc gccagtgtcc gcaacggatg
     2041 ccggatggaa acccgggaga gacggttcat caggatggga cggagccggg cggtacccgc
     2101 ccggctcctt tggcgtgtgg cctcgctctc ctactgctcg ccgaaggcct tcatggtcac
     2161 tttccggacc gtcctgcccc ggtagttccc gagccgcgga ccggcgccga cgaagtcgat
     2221 ggcgacggtg acgtcgaagc tgctgtccac gtggaaggtg cgattcagct gccgggtcag
     2281 ggtcgtgtga ccagggctgg ggtaaccgtt cgcggtgccg cccctgatcg tgaaggaccc
     2341 gaagtccgga tcgttggtga tcccgccctg gatgcggtac atgtcggttc tgaagctctg
     2401 caccggagcc ccgggcgtgc ggggcgcggt gtgcgtctcg acttgaaggg gaatggtgag
     2461 cgtacggctc cagccgctca gggcgccggt ccccgtcacc tggaagacga tatccgaaga
     2521 aaagacttct ctctctccac ccagagagcc gcccgacgag ccacaagccc ctcccgtgcg
     2581 gcagatgaaa ttccggtgga tcggcgccag gatgatctcc gtgcccacgg gaagaccggc
     2641 aatgatcttg tggacatcgt tggggcccag gtactcgcaa ttggcgggcg gcagcgtcac
     2701 cgtcccggag ccgttgtcca cctcgaagca ctgcggaggc ggcggcggcg cggtgtgacc
     2761 gatcccgacc accagggcca ggccgaggct catgaaaatc gtgcgagtcc agccgcttcg
     2821 tgtcgtcttc atgcgactct cctttgaaaa aagaatctcc tggcatagcc accagagagc
     2881 gtgggggcgt ggccggccga ccgaggagtg ccctcgctat ttcatgtgca tgaagttata
     2941 aaatgcgagc cgctaaggac aggtgagatt cagccagccc ctccttgaag gagaggccgg
     3001 ctggtcagga gctcctgaga ggggagagtg cgatcgagga ggctactccg gcaccaccgc
     3061 cgtcgcctcg atctcaactt tcgcccggtc ttcgaggagc gccgccacca ccaccagggt
     3121 catggccgga tagtggcgcc ccatgacgtc gcgccaggcg cggccgatct ctttctggtt
     3181 tgccaggtag gctgccttgt cggtgatgta ccaggtgacg cggaccagat gggccggctc
     3241 ggcgccagcc tcgcgcacca ccgcggcgac attttcgagc gcccgggcgg tctgggccac
     3301 gaagtcgtcg gtctcgaagg cgcaggtctc cgggttccag ccgatctggc cggcgacgaa
     3361 gacgagacgg ccgctcgtcg ccatcccgtt gacgtagccc ccgggcgccg cccagccggc
     3421 gggctggagg atgtccggca cgctactctt ccctctcgcg cagacggaag cgctgcagct
     3481 tgccggtcag tgtcctgggg aggctgtcga cgaactccac cgatcgcgga tatttgtagg
     3541 gggcgatctc ggccttgacg tggtcctgga gctccttgac cagctccggc gcggcggcga
     3601 agccctgacg cagcaccacg aacgccttga cgatctggcc gcgctcctcg tcgggcaccc
     3661 ccaccactcc gcactccgcc accgcggggt gctccagcag cacgtcctcc acctcggggc
     3721 ccgagatgtt gtggcccgcc gagatgatca tgtcgtccgt gcgtgcctgg taccagaagt
     3781 agccgtcctc gtccatgacg taggagtcgc cggtcaggtt ccagccgttc tgcacgtagc
     3841 cgcgctgtcg ctccaggtcg tcgagatagc ggcagcccgt cggccccttg accgcaagcc
     3901 gaccgatggt ccccggcggc acctcgtgga agccctcgtc caccactcgg gcctggtagc
     3961 ctggcaccgc cctccccgtc gagccagggc ggatgtcgtc tcccgaggag ctgatgaaga
     4021 tgtggagcat ctcggtggag ccgatgccgt cgatgatctt gatccccgtc gtctcctccc
     4081 atagctcgaa ggtcgacttc ggcaaggtct cccccgccga cacgcacttg cggagactcg
     4141 agatgtcgaa gcccttcgcc atccctgcca tggcccggta tgccgtgggc gcagtgaagc
     4201 agaccgtggc ccggtggtcc tggatcccct gcaggagctg cggcggcgag gcctgctcca
     4261 agagaatggt cgaagcgccg gcacgcatcg ggaagaggag cagcccgccc aggccgaagg
     4321 tgaaggccag cggcggcgag ccgcagtaga tgtcgtccgc ctcgggcttg aggatgtagg
     4381 gggggaaaca atcgcagacc gccagcacgt cgcgatggaa gtgcatggtc cccttgccct
     4441 gccccgtggt gcccgaggtg aaggcgatga gcgccgtgtc gtccgccgcc gtgtcgcagt
     4501 tgtcgaaggt ggtgggcttg ccctgcatca gcgactctag cgagctcggg ccggcgctgt
     4561 ggaaagggat cacccgggcc ccctcgcggg ggaagccggc gccggtcgtg gccatggcct
     4621 gctcgcagtc gccggcgatc cgggagtcgg tcagagccag ccggatcttc gccttgtcgg
     4681 cgatgtaggt cagctcccgc acccgcagca gcggcatggt cgcgaccacc acgccacccg
     4741 ccttgagcac ggcgaaccag caggccgcca tcatcgggtt gttgggacca cgcagcagca
     4801 cgcggttacc cggcacgacg ccgaggtcgt ccaccagcac gtgggcgata cggttggcgg
     4861 tctccaggag ctgctggtag gtccagcggc cgccggggaa gtggaagacg gtgcgctcgc
     4921 cgcagccggc cgccaccagc tcgtcgagga gctcgacggc gcagttcagc cgcggtgggt
     4981 agcgaagctc cggcacgccg gtgtagatca tctccggcca cagctcccgt ggcggcaggg
     5041 cctcgcggca gaaggtgtcc acgtgggcac tcggctcggg cgcggtcgat tcagccatga
     5101 tgggtgctgc ctcctgtaga cgctggcgcc ggttgcgacg gtggtgcggc ggcgaaggtc
     5161 tggccggcga tgaccagccg ctggatctcg ctggtccctt cgtagatgcg cagcgcccgg
     5221 acctcgcggt agagcttctc caccggctcg cccgacatca cccctctccc tccgagcaac
     5281 tgcacggctt catcgatgac ccgctgcgcc gcctcggtgg cgaacagctt cgccatggcc
     5341 gcctcgcggg tgacgcgcgc cgctccggtg tcccgcgtcc aggcggcgcg atagaccagg
     5401 agcgccgagg cgtccacggc ggtggccatg tcggcgatct tggcctgggt gagctggaac
     5461 tcggccagcg gcttgccgaa cgccttgcgc cgcacggcgt gagccagggc ctcgtcgagg
     5521 gcccggcggg cgaagcccag ggccgcggcg ccgacggtgg aacggaagac gtcgagcgtc
     5581 cccagcgcga ccttcaggcc cttgcccggc tcccccacca gggcgccctc cggcacccgg
     5641 cagtcctcga aggacagcgt gcccagcgga tggggcgcca ggacgtcgat acggcgggtc
     5701 accttcaggc cggggttgtc cgcctccacc agcagcgcgg cgaacgcctt ctccccctct
     5761 tctggcaggc gacagaagac gacgtagaag tcggccaggc cggcgttgga gatccaggtc
     5821 ttctcgccct cgatcaccca gccgtcgccg tcgcggcgcg ccgtggtggt catcgaggag
     5881 acgtcggagc cggagtcggt ctcggatagg gcgaaggcgg agatggcccg gccggcggcg
     5941 acgcggggga gccaggtgcg ccgctgctcc tcagagccga agagggagag cgggccgctc
     6001 cccagcccct ggagagcgaa ggcgaagtcg gcgatccccg agtggcggcc cagagtctcg
     6061 cggatcaggc agagcgcgcg gacgtccagc cggccggtgc ccccctgctc cgcgccgcgg
     6121 ggcacggcgt agcggagcca tcccccttga ccgaggcggt ggacgatctc gcggcaggtg
     6181 gcgtccaacg cctcgtcctc ccagccttcc aggggagcga tctcccgctc gcaccaggcg
     6241 tcgagctcgg ggacgagggc gcggtgggcg tcgtcgaaga acggccagga aagaaaggtg
     6301 acgtcgggca tcagttgcct tcgaagaccg gcttctcttt cgcggcgaac gcgcggaagg
     6361 cgcgggcgaa gtcctcggtc tgcatgcaga tggcctgggc ctgggcctcg gcctcgatgg
     6421 cctcgtccac ccccatgctc cactcctggt ggagctggtt cttggtcatg gcgtgggcaa
     6481 aggtcgggcc atgggcgatg gagcgcgcca gggctatggc ttcggccagg aggtccccgg
     6541 gctcgacgag gcggttgaag aagccccagc gctcggcctc cgcggcctcc atgaagcggc
     6601 cggtgtagag gagctccgaa gcccgcccct gcccgatgat ccgcggcagg atggcgcacg
     6661 cccccatgtc ggcgccggag aggccgacgc gggtgaagag gaaggcgacg cggctgcgcg
     6721 gcgtgcccag gcgcatgtcg gaggccatgg cgaggatcgc ccccgcgccg gcgcagatgc
     6781 cgtccaccgc ggcgatgatc ggctgcgggc agtggcgcat ggtgcgcacc agctcgccgg
     6841 tgagacgggt gaactcgagc agccggtcca tccgcccctc ctccttcatg cggacgagcg
     6901 ggccgatgat ctcgtggacg tcgccgccgg acgagaagtt ctcgccggcg ccggtcagca
     6961 ccacggcctt gacctcctcc acatggcgga ggcggtggaa ggtgtcggac agctcccggt
     7021 aggactcgaa cgtcagcggg ttcttgcgct ccggccggct gatggtgacc gtggcgacgc
     7081 ggtcctcgac gctccagccg aagtgccggg gctggatctc ttccagggag atacggttgt
     7141 tcaaggagtc ctcccctctc tgggcaaacg gctctcgcaa cggccgggcc ggatgctagc
     7201 acgagcccct tccctgccgc ccggcttgac gtcagaatgc cctgccatgt ccgccctcta
     7261 ccccgatccg ctgctctcct ccagtctgta cggcaccggg cggctggacg cgctgatcgt
     7321 cgagatggtg gcccccttct ggcgggaggt gagggaccag gccggggacg cttatctatg
     7381 gatgcttcgc tacggccgcc ggggcgagca cctcaagctc cgcctgcacg gcccggagga
     7441 cctccggccg ctcctcgcac cccaacttga agcggcggcg gagtcgtact tcgccgcccg
     7501 ggagcggcgg ccgcgtccgg ccgagccggt gaagcggcgg ccggcgccgc cgctcgacga
     7561 ggaggaccac ggccgggacg accaccctga cgacagcttc ctctggaccc gctcccggcg
     7621 cgtccccatg gtcctgggcg ccgcccccct gctccacgac gaccgctacg ccgccctctt
     7681 caccacctgc ctgggccgcg cctgcgaact gatcctggac tggctcgagt tggaggacgg
     7741 cggcacggtc tccttccgca gccgccaggg caccctcatg atgctcctct ccaccggcct
     7801 cgcggcggcg tttcctgacg aggaggagcg cgtgcgatac ctgacctatc accgggactg
     7861 gctggtgcgc tcccccgtcc tctacacgag aggccggatt gagaaggcgc gcgccatcct
     7921 cgaccgcttc gatcaagagg cggagcggat gggctccgcg gcccaggtga gcctcgcgga
     7981 ggtggtgtac gccgatgggg acgccgagga cgacgagccc gccgaggcgg cctggcgccg
     8041 ctccgtcgcc gagctgcgcg cctacctgga gccgctccag ggggatcccg tctacgacct
     8101 cgaccccttc gtcgaagggc cgctccaccc ctgtctgttc aagctcttcc actgcgtcgc
     8161 caaccagatc gggctgaccg cgttgaacga agcgctgacc tggcatctcc tggtccacgc
     8221 cgccgacgag gagagcgggg aattccgatt ggtgccggca ggggtgtgac atggacgaca
     8281 agagcttcaa cttcgagaag gactacgact ggaccgccct cgttgctggc atggggggcg
     8341 gcatgggcgg cggcatgggc ggcggcgccc agggctggct cgacgagtac tcccgcgtcg
     8401 gcagcgagat cttcgacgac atccgctggg ggttgggtct gctgcggcgc cgcgagatgg
     8461 aggaaggact ggcccgcctc gaccgggcac gctcgcggtg ggagccgctc cgggattcgt
     8521 caccggcgat ctatcacgtt ctaggccgct actatcacgg cgccctcgcc tactggcact
     8581 attgcgtcga cgacttccct cgggcggacg agacgctgcg gcaggcgctc gatgcggtgc
     8641 ggtccgcggt ggaggccgag ccgttcctcc tccccttcgc cgcggtctcg atggacatcc
     8701 cgctgaagca ggtgcagatc gctcgagccc tgc
//
LOCUS       DASPJI010000476         6037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_837477, whole genome shotgun sequence.
ACCESSION   DASPJI010000476 DASPJI010000000
VERSION     DASPJI010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837477"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VEL74_13010"
     CDS             <1..317
                     /locus_tag="VEL74_13010"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HYG63495.1"
                     /translation="DPLQEYKKESFELFQSMKDRVEDDIIQRLFRYEPVTEEQLLEQR
                     RRQEQAAPKVQFSAPPKVEGPRPQVAAAQKQELKIGRNDPCPCGSGKKYKKCHGAAVT
                     VG"
     gene            450..1919
                     /gene="guaB"
                     /locus_tag="VEL74_13015"
     CDS             450..1919
                     /gene="guaB"
                     /locus_tag="VEL74_13015"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HYG63496.1"
                     /translation="MTAFTPPLALTFDDVSIIPGLSEVHPNEVDLRTPVCRGINLNIP
                     ILSAAMDTVTESRLAIALAQEGGMGIVHKNLPLEIQAEEVDKVKRSESGMIVDPVTMR
                     PDQSIREALAMMARYKISGVPVTDHQGHLVGILTNRDLRFEDDLDKKIQELMTKEDLI
                     TVPEGTTLDQAKLLLHRHRIEKVLVVDTDGNLKGLITVKDIQKNIQYPNACKDELGRL
                     RVGAAVGASGDYLERAAALVKAKVDLLALDSSHAHSRGVLDAAARLRERFPEVKLVIG
                     NVATAEGALALIERGADAVKVGIGPGSICTTRVVTGAGIPQITAVVECARAAAEHGVP
                     IIADGGIKFSGDVTKAMAAGAHSVMIGSLFAGTEESPGETILYQGRTFKAYRGMGSLS
                     AMSRGSADRYFQEEAGSLAKMVPEGIEGMVPYKGSVHQMLPQLTGGLRSGMGLAGCAT
                     IEELRTKARFVRISAAGLKESHAHDVVITKEAPNYWVER"
     gene            2055..4121
                     /locus_tag="VEL74_13020"
     CDS             2055..4121
                     /locus_tag="VEL74_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63497.1"
                     /translation="MRDRRRLCLTVAAMLMVPSLAAAIEPPQDLPQAFVGKQFSAPEL
                     EVQPSLEHFEPGAKAASLDELQRFFVFHSEDWNVRWDVRADRPNLIEGAGVPLIPGRG
                     NKLRREDVKTGHSGALRLADVEALLRGFLADFPEVLNVSGLDLRLNPAASVNVGNEKQ
                     LWFIELQQHHNDVPVDGATAFFRINNGNLIQFGVERVSEVKIPSTPRLSREAAFAKVL
                     RAGGIGVGEVTEILDPGTLKIVPLLAMGEKPAERYAGMRGHGYRHALVWQLSFRRADD
                     PITYQALVDARSGRVMQVTDLTSYAAAQVTGGVYPKTNLDPEVVVPLPFTNVTNGAAK
                     VTDANGTYDYTGGAATATLNGKYINIVDNCGAISLANSTTGNIAFGTSGGTDCTTPGI
                     GGAGNTHSTRSAYYHLTNINRKAATYLPGNAWLNGTVTANVNVNQTCNASWNGTAMNF
                     YRSGGGCGNTGELAAVFLHEWGHGMDQNSGGAASDKGTGEAVGDTFAFLETKDSCIGH
                     NFRPGIPCSNCGTCTGVRDMAPFSLGGARTIAKPSTVTDNSGINCDAWSCPYYVFFFI
                     GAYQGPMGYEGHCESYIASTANWDLAQSLIARYGNAAGWAAMDQIWYESLTPSKAAYQ
                     VAAGGKCNPAATVNGCGATNWYTVYLAVDDDDGNLANGTPNGCRIWDAFNAHGIACGS
                     RPACTV"
     gene            4228..5238
                     /locus_tag="VEL74_13025"
     CDS             4228..5238
                     /locus_tag="VEL74_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016951978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG63498.1"
                     /translation="MKLRKKPKTPEVVVITGASAGVGRATAREFARRGARIGLVARGR
                     EGLEAARREVEELGGQGMVLPLDVSDADQVESAAARVEEELGPIDIWVNNAMVSVFSP
                     VKEMTPEEYRWVTEVTYLGYVYGTLAALRRMLPRDRGSIVQVGSTLAYRGIPLQSAYC
                     GAKHAIQGFCDSLRCELLHDGSNVRLTMVQMPALNTPQFGWVKSRLPRKAQPVPPIFQ
                     PEVAARAVVWAAHHDRREVWVGGRTVIGIVGNRIAPWYADRYLARTGYDSQQLESPED
                     PNRADNLWDPLPGDHGAHGSFDDRATGRSPQLWAATHRGLLASLMGAGVGLLWAWRSV
                     KT"
     gene            complement(5228..>6037)
                     /locus_tag="VEL74_13030"
     CDS             complement(5228..>6037)
                     /locus_tag="VEL74_13030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG63499.1"
                     /translation="QVFWNLLHNAIKFTPAGGTITVRSRIARPQGELPGELLVEVSDS
                     GIGIEPEVLPRIFNAFEQADRLITRRFGGLGLGLAVSRAIVELHGGTLTAASPGRGQG
                     ATFSVALPVEEAQGTESAAAQLPGAVPPERSTSGGRAYPLHLLLVEDHADTAAAMADL
                     LHGFGYRVTVAGSVAEALQAATAAQSGSDRLDLLVSDLGLPDGSGLDVMRELAGRWGL
                     KGIALSGYGMEEDLRKSREAGFEKHLTKPVNIQTLDTTIRQVAGEGKPSTS"
BASE COUNT         1002 a         2138 c         2025 g          872 t
ORIGIN      
        1 gcgacccgct ccaggagtac aagaaggaga gcttcgagct gttccagtcg atgaaggacc
       61 gggtggagga cgacatcatc cagcggctgt tccgttacga gccggtgacc gaggagcagc
      121 tcctggagca gcggcggcgg caggagcagg ctgcgcccaa ggtgcagttc tcggctccgc
      181 cgaaggtgga gggcccccgg ccgcaggtcg ccgcggccca gaagcaggag ctcaagatcg
      241 ggcgcaacga cccctgcccc tgcggctcgg gcaagaagta caagaagtgt cacggcgcgg
      301 ctgtcaccgt cggctgagct ggagatcgga cccatgacac ctgggcggcc tgcgggccgc
      361 ccttttcgta gagggatcgg tggtatcctc tgccgcgatc cttcgcccct cgtaccaact
      421 ttcaggaaaa accgagacgc atggttgaga tgacggcctt cactcctccc ctagccctca
      481 cgttcgacga cgtctctatt atcccgggcc tctccgaggt ccaccccaac gaggtggacc
      541 tgcgcactcc ggtctgccgc ggcatcaacc tcaacatccc gatcctctcc gccgccatgg
      601 acaccgtgac cgagtcgcgg ctcgccatcg cgctggcgca ggaggggggg atgggcatcg
      661 ttcacaagaa cctccccctt gagatccagg ccgaggaggt ggacaaggtc aagcgctccg
      721 agtccggcat gatcgtcgac ccggtgacga tgcggcccga ccagagcatc cgcgaggcgc
      781 tagccatgat ggcgcgctac aagatttccg gcgtgccggt gaccgaccac caggggcacc
      841 tggtgggcat cctcaccaac cgggacctcc gcttcgagga cgacctggac aagaagatcc
      901 aggagctgat gaccaaggag gacctgatca ccgtgcccga ggggacgacc ctcgaccagg
      961 cgaagctcct cctccaccgc caccgcatcg agaaggtgct ggtggtggac acggacggca
     1021 acctcaaggg gctgatcacg gtcaaggaca tccagaagaa catccagtac cccaacgcct
     1081 gcaaggacga gctcggccgc ctgcgggtcg gggcggcggt cggggcctcg ggcgactacc
     1141 tggaacgggc ggcggcgctg gtcaaggcca aggtggacct gctagcgctg gactcctcgc
     1201 acgcccacag ccgcggcgtg ctggacgcgg cggcgcggct gcgcgagcgc ttccccgagg
     1261 tcaagctggt gatcggcaac gtggcgaccg ccgagggggc gctggcgctc atcgagcgcg
     1321 gggccgacgc ggtcaaggtg gggatcgggc cgggcagcat ctgcacgacg cgggtggtca
     1381 ccggcgccgg catcccgcag atcacggcgg tggtcgagtg cgcgcgggcg gcggccgagc
     1441 acggcgtgcc gatcatcgcc gacggcggca tcaagttctc cggcgacgtg accaaggcga
     1501 tggcggccgg cgcccactcg gtgatgatcg gctcgctgtt cgccggcacc gaggagagcc
     1561 cgggcgagac catcctctac caggggagga cgttcaaggc ctaccgcggc atgggctcgc
     1621 tctcggcgat gtcgcgcggc agcgccgacc gctacttcca ggaggaggcc ggcagcctgg
     1681 cgaagatggt gccggagggg atcgagggta tggtgccgta caagggctcg gtgcaccaga
     1741 tgctccctca gctcaccggc ggcctgcgct ccggcatggg cctcgcgggc tgcgccacca
     1801 tcgaggagct gcgtaccaaa gcccgcttcg tccgcatctc cgccgccggc ctcaaggaga
     1861 gccacgccca cgacgtggtc atcaccaagg aagcgccgaa ctattgggtg gagcggtagc
     1921 ggcagctacc gttcccccct tcttctctct tcttcctctc ccctaccccc tctccgtccc
     1981 ggagtgtccg cctcccacac cttcaggtgt gtaggcgaac gctgtattcc cttgaaagga
     2041 gaggttcgag agtcatgaga gaccgtagac gtctgtgcct caccgtagcg gcgatgttga
     2101 tggtgccttc gctcgctgcc gccatcgagc ccccgcagga tctgccgcag gccttcgtcg
     2161 ggaagcagtt ctccgccccc gagctggagg tccaaccgtc cctggagcac ttcgagcccg
     2221 gcgccaaggc ggccagcctc gacgagctcc agcgcttctt cgtctttcac tccgaggact
     2281 ggaacgtccg ctgggacgtc cgcgccgaca ggccgaacct gatcgagggc gccggggtgc
     2341 cgctgatccc cgggcgcggc aacaagctgc ggcgcgagga cgtcaagacc ggccactccg
     2401 gcgccctgcg gctcgccgac gtggaggcgc tgctgcgcgg cttcctggcc gacttccccg
     2461 aggtgctgaa cgtctccgga ctcgacctgc ggctcaatcc agcggccagc gtcaacgtcg
     2521 gcaatgagaa acagctctgg ttcatcgagc tccagcagca ccacaacgac gtgccggtgg
     2581 acggcgccac cgccttcttc cgcatcaaca acggcaatct aatccagttc ggggtggaga
     2641 gggtgagcga ggtgaagatc ccctccacgc cgcgcctgag ccgcgaggct gcgttcgcca
     2701 aggtgctccg ggccggcggc atcggcgtcg gcgaggtcac cgagatcctc gatcccggca
     2761 cgctcaagat cgtccccctg ctggcgatgg gtgagaagcc ggccgagcgc tacgccggca
     2821 tgcggggcca cggctaccgg cacgcgctgg tctggcagct ctccttccgc cgggcggacg
     2881 acccgatcac ctaccaggcg ctggtcgacg cccgtagcgg cagggtgatg caggtgaccg
     2941 acctgacgag ctacgccgcg gcgcaggtca ccggcggcgt ctaccccaag accaacctgg
     3001 accccgaagt cgtggtgccc ctgccgttca ccaacgtcac caacggcgcc gccaaggtca
     3061 ccgacgccaa cggcacctac gactacaccg gcggcgcggc cacggccacc ctgaacggca
     3121 agtacatcaa catcgtcgac aactgcggcg ccatctccct cgccaacagc accaccggca
     3181 acatcgcctt cggcaccagc ggcggcaccg actgcacgac gccgggcatc ggcggcgcgg
     3241 gcaacaccca ctcgacgcgc tcggcttact accatctgac caacatcaac cggaaggccg
     3301 ccacctacct gccgggtaac gcctggctga acggcaccgt caccgccaac gtcaacgtca
     3361 accagacctg caacgcgagc tggaacggca cggcgatgaa cttctatcgc tcgggcggcg
     3421 gctgcggcaa cactggggag ttggcggcgg tgttcctcca tgagtggggg cacggcatgg
     3481 accagaactc gggcggcgcg gcgagcgaca agggcaccgg cgaggcggtg ggagacacct
     3541 tcgccttcct ggagaccaag gactcctgca tcggccacaa cttccggccc gggattccct
     3601 gctcgaactg cggcacctgc accggcgtgc gcgacatggc ccccttctcg ctgggcggcg
     3661 ccaggaccat cgccaagccg agcacggtga cggacaacag cggcatcaac tgcgacgcct
     3721 ggtcctgccc ctactacgtc tttttcttca tcggcgccta ccaggggccg atgggttatg
     3781 aggggcactg cgagagctac atcgccagca ccgctaactg ggacctcgcg cagtcgctga
     3841 tcgcccgcta cggcaacgcc gccggctggg cggccatgga ccagatctgg tacgagagcc
     3901 tgacgccgag caaagccgct tatcaggtgg ccgccggcgg caagtgcaac cccgcggcga
     3961 cggtgaacgg ctgcggcgcc accaactggt acacggtcta cctggcggtg gacgacgacg
     4021 acggcaacct ggccaacggc acccccaacg gctgccggat ctgggacgcc ttcaacgccc
     4081 acggcatcgc ctgtggcagc cgtccggcct gtacggtcta gacggcaccc tttccctctc
     4141 cggcgggacc ggaccccggc ccggtcccgc cttcggcacc ccccttgcag cggttgacct
     4201 tccggacgga gcaggagggg accgctcatg aagctcagga agaaaccgaa gacacccgaa
     4261 gtcgtggtca tcaccggcgc ctccgccggc gtcgggcggg cgacggcccg ggagttcgcc
     4321 cggcgcggcg cacggatcgg cctggtcgcc cgtgggcggg agggcctgga ggccgcgcgg
     4381 cgggaggtcg aggagctggg gggccagggg atggtgctgc cgttggacgt gtccgacgcc
     4441 gatcaggtgg agagcgccgc cgcccgtgtc gaggaggagc tcggcccgat cgacatctgg
     4501 gtcaacaacg ccatggtgtc ggtgttctcg ccggtcaagg agatgactcc cgaggagtac
     4561 cggtgggtga ccgaggtgac ctacctcggc tatgtgtacg ggacgctcgc cgcactgcgc
     4621 cgtatgctcc cccgcgaccg cggctcgatc gtccaggtgg gctccaccct cgcctatcgg
     4681 ggtatccccc tgcagtcggc gtactgcggc gccaagcacg ccatccaggg gttctgcgat
     4741 tcgctgcgtt gcgagctgct gcacgacggc agcaacgtgc gccttaccat ggtccagatg
     4801 ccggccctca acacacccca gttcggctgg gtcaagagcc gcctgcccag gaaggctcag
     4861 cccgtgcccc ctatcttcca gcccgaggtc gcggcccgcg ctgtcgtgtg ggcggcgcac
     4921 cacgaccggc gcgaggtctg ggtgggcggc aggacggtga tcgggatcgt gggcaaccgc
     4981 atcgccccct ggtatgcgga ccggtacctg gcccgcaccg gctacgactc ccagcagctc
     5041 gagagcccgg aggatccgaa ccgggccgac aacctatggg atccccttcc cggagatcac
     5101 ggcgcccacg gcagcttcga cgaccgagcc acaggccgtt ccccccagct ctgggccgcc
     5161 acccaccgcg ggctcctcgc ctcgctgatg ggcgccggag taggactgct ctgggcctgg
     5221 cggagcgtca agacgtagag ggcttcccct cgccggccac ttggcggatg gtggtgtcga
     5281 gcgtctggat gttcaccggt ttggtgaggt gcttctcgaa tccggcctcg cggctcttgc
     5341 ggaggtcttc ctccatgccg tagcccgaga gggcgatccc cttgaggccc cagcggcccg
     5401 ccagctcgcg catgacgtcg aggccgctgc cgtccggcag gcccaggtcg ctcaccagca
     5461 ggtcgaggcg gtcggacccg ctctgggccg cggtcgccgc ctgcagcgcc tccgccaccg
     5521 agccggccac cgtcacccga tagccgaagc cgtgcaggag atcggccatc gccgccgccg
     5581 tgtcggcgtg gtcctcgacc agcaggaggt gcagggggta ggcccgcccc ccggaggtgg
     5641 agcgctccgg cggcaccgca cccgggagct gggcggccgc ggattccgtg ccctgcgcct
     5701 cctccaccgg cagagcgacg gagaacgtgg cgccctggcc ccgtcccggg ctcgccgccg
     5761 tcagcgtgcc gccatgcagc tcgacgatcg cccggctcac cgccaggccc aggccgagcc
     5821 cgccgaagcg gcgggtgatc agccggtccg cctgctcgaa ggcgttgaag attcgcggca
     5881 gcacttcggg ctcgatgccg atccccgaat cggagacctc caccagcagc tcgccgggga
     5941 gctcaccctg agggcgggcg atcctcgacc gcaccgtaat ggtccctccg gcaggggtga
     6001 acttgatcgc gttgtgcagc aggttccaga agacctg
//
LOCUS       DASPJI010000477         9412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_27017, whole genome shotgun sequence.
ACCESSION   DASPJI010000477 DASPJI010000000
VERSION     DASPJI010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27017"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VEL74_13035"
     CDS             <1..759
                     /locus_tag="VEL74_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63500.1"
                     /translation="GWNIEAGELAVTPLEPVESSLAVEVTLTGEPRDGVTAPLLAPRG
                     ASRALWFAKALAQGDGLLELEQAGATREPVASETAALPQAFRDMAGATVAVTDPARPP
                     RWQAVWAEGTDVLAVQVDRLLVDVALGKSGRASYQLWAEVRNRGAQQLVLTLPAGFEL
                     AEGRRDGLPVAPGLAPAAGHGFAVPLRTAEEAQVIHLTGVLPLTWPEGDGDLAVPLPA
                     LSAPAARVEVRVVLPGRRTYTLPGRVRWAARLGP"
     gene            759..971
                     /locus_tag="VEL74_13040"
     CDS             759..971
                     /locus_tag="VEL74_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63501.1"
                     /translation="MAPAPAAGPQSSQIAQQLLASRATSPSAPAALFESPPGFVVVPA
                     TWSALSAAPSALVLHVESRKVRQPWF"
     gene            962..3139
                     /locus_tag="VEL74_13045"
     CDS             962..3139
                     /locus_tag="VEL74_13045"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYG63502.1"
                     /translation="MVLRRWIAGLFLIVLMSLMSVRAWGQGAGPGEVSLPLADYLALA
                     GRAEAAEKERAERAAGREAPVAEVTAQRVSLVAGEEEAEVTSELEVLVQGHPSKQVFL
                     PLAGIAERVEVTPATAALTATEGGAMLVTPGPGKYLVRVHGRLPVARAGGVGRLPLAP
                     VVAPVAEVQADLPADAGWSSPGTVVVEEREQDGRRRVRLAAQRGKTAVLELRRRVDGS
                     DADRLLARAVVLTLFQLRPDGARRHDVVLYEVARGGLASFAVELPPGLEVEQVGTDEG
                     AVVPLAEARRLTVHRQKQLQGAGYLVLTSTPPSGVSTGQVPFAFALPGVEVRARYLAL
                     ASSVAADVQPVPEKSWARVDLDDLPAELRGALGGRGLSAAWRLLAPPAAPAASAGLAL
                     AVAELPKAPELEEVVRARETMTLVTLDGTVLHRDLFTVEGGGEVFDLTLPAGATLWSA
                     RVGEQPVRPVERGGRLSVPLGFQSGLARVEVVSVLPGEIPEGRSRLALELARVEAPVL
                     DHRWRLLLPEGARYRFRDGDLLPAWEMVTFVTKSEAWQSLGQGQAGVRGRVRDEKGRP
                     LAGATVTLLSRGLQAESTAVTGADGGFAFTLLAAGEYEMTASSEGHQPEVYRFSLGSR
                     EVRPAYIQLTTGQVVSEEILVSTPPSFDTTTIGENFDYESEVESLAIQERYFENVALL
                     SNGLVGGVKPLPVTIPETGKVLILTGVLPPERVAAELEVRAKR"
     gene            complement(3199..5313)
                     /locus_tag="VEL74_13050"
     CDS             complement(3199..5313)
                     /locus_tag="VEL74_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020115995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYG63503.1"
                     /translation="MDAFDRLHPALQHHIVNSLGWRTLRPLQESAIEPVLAGSHALLV
                     APTAGGKTEAAVFPLFSRMLSEDWQGLSVLYVCPLRALLNNLEVRLSGYLGLLGRRAG
                     LWHGDVGQGPRRQIQREPPDLLLTTPESLEVMLTSRGIDHAGLFYNLRSVVVDELHAF
                     AGDDRGWHLLSVLERVSRVAGRDLQRIGLSATIGNPPQLLTWLAGSSPGPRTVVAPDS
                     GQTVEADVQIDAVGSLENAAVVLSRLYRGEKRLVFCDSRSRVEDLAFRLRELGVETHV
                     SHGSLGIDERRRAEQAFAEARDCVIVATSTLELGIDVGDLDRVIQIEAPATVASFLQR
                     LGRTGRRQGTKRNMLFLTTAPETLLRAAAIGLLWSEGYVEPVEPPALPFHIYAQQLLA
                     LAYQLPGMPRGAWSTWLDPMPGFAVLPAGARRSVVDHLLSEGFLHEDSGLLGFGPESD
                     RRFRGKGFLELLSVFDAAPSVRVLHGRTELGFVDDATFQLETPGGTVLLLGGRSWRVV
                     HVDWSRREAWVEPIEIPGRSLWLGDGLPLGFALCQAQKRVLLGEGHEGLLTLRARQKL
                     HEFRGRFPWLSQEATSLERGAPGRAHWWTFAGLKANATLADHLAGRGVSVLSRDNLSI
                     TLGISEGLPPLQELLPVPSEAAQLRPASLVADAMESLKFAFCTPRDLCFTALSRRLAD
                     PQGVAATVAVPVVQVQGDESFS"
     gene            complement(5317..5733)
                     /locus_tag="VEL74_13055"
                     /pseudo
     CDS             complement(5317..5733)
                     /locus_tag="VEL74_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
     gene            6018..6539
                     /locus_tag="VEL74_13060"
     CDS             6018..6539
                     /locus_tag="VEL74_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63504.1"
                     /translation="MRSFDDRSSSAIHPNAFDGPFLRRLAERDEPPTAGDADVAGPWR
                     IEEIPQHGFGLFRLGESLSRGFAPVAIFPSRWLALLMAAILPGTGRDPLVRLEKLLDV
                     EGGYPLVLPGPDGGEVVGRFQLFDEAAVDALNVAVHLLQAPESLAYLLEAAGAVTLER
                     CGAILDERTAAPQ"
     gene            7117..>9412
                     /locus_tag="VEL74_13065"
     CDS             7117..>9412
                     /locus_tag="VEL74_13065"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYG63505.1"
                     /translation="MAQTFHGGLSRSLVVLVALVATAALGASCRAANESPAIEVLTPG
                     ETVTARLAEGAVHAYGISVPAGHLLHLVADQEGVDVVLTLRRSTGEPLLEIDSPSGSW
                     GPEELWWIAPDSGVWRLEVRPWTPAPRGRYQLRALAVRPASSRDGLRAAAARNEWEAG
                     RLLAAGDAAAARRELTAAREMWRRAGVPLQEALAGLRLAAVSEAVEDAAGEVESYRSA
                     LTGLRRLGQPRLEVFSLLRLGEALRKAGDLAGAWEAQEEALGLAQRRGLKEEAAFALN
                     NLALILEARGETGAARERYRHTAALFRRLGLKAEEAMALHSLGTCSSLLGRLKEAEAA
                     LTAALALRRSLGDVRGEGATLTELGWVYHLRALAGGGETAREKARGFLQEALLRRREA
                     RDSLGESGTLDRLGTVYRHSGLWNEALACYQRSLVLIRDTPPGRRQANTLSNLAELWL
                     GRGRPAKARELATEAVARFAALPVRDAHGEIHARYLLGRAAAGLGDLAGARRELEQVL
                     STVEALRSELGDQTLTLAFFAQRQLYFEGAIETLMNLDALYPGQGFDIAALETSERAR
                     MRTLLDSLAARRRRAQGKEESDEARPMTAERIQRELLDGDTVLLEVALGEERGFLWVV
                     SRERVEAFRVPGWRVLRPLVEEAYRGLRGGGGREASRQLVELLLGPAVDRLDGGLATR
                     RLAVVTDGLLAHIPFSALPWGEKGRPLVESYEIVRLPSATALAMLRQRAALRPAAEAG
                     ATAVVADPVFSTLDPRVHRAGGAPP"
BASE COUNT         1276 a         3267 c         3496 g         1373 t
ORIGIN      
        1 ggctggaaca tcgaggcagg cgagctggcc gtcaccccgc tcgagccggt ggagtcgtcg
       61 ctggcggtgg aggtgaccct caccggcgag ccgcgggacg gcgtcaccgc gccgctgctc
      121 gcgccgcgcg gggcctcccg cgcgctctgg ttcgccaagg cgctggccca gggggacggc
      181 ctcctggagc tggagcaggc cggggcgacg cgcgagcccg tagcgtcgga gaccgccgcg
      241 ctgccccagg ccttccggga catggccggc gcgacggtgg cggtcaccga tccggcgcgg
      301 ccgccgcgct ggcaggcggt gtgggcggag gggacggacg tcctggccgt ccaggtggac
      361 cgcttgctgg tggacgtggc gctggggaag tccggccgcg cctcctacca gctctgggcc
      421 gaggtccgca accgcggcgc ccagcagctc gtcctgaccc ttcccgccgg cttcgagctc
      481 gccgaggggc ggcgcgacgg cctgcccgtg gcgccgggcc tcgcgccggc cgcgggccac
      541 ggcttcgccg tgccgttgcg gacagccgag gaggctcagg tcatccacct caccggcgtc
      601 ctgcccttga cgtggccgga gggggacggc gacctggcgg tgccgctgcc ggcgctgtcc
      661 gccccggcgg cccgggtgga ggtccgggtg gtgctgccgg gccggcggac ctacacgctg
      721 cccgggcggg tcaggtgggc agcccgcctc ggcccttgat ggcaccggca ccggccgccg
      781 gcccgcagtc ctcccagatc gcgcagcagc tcctggcgag ccgggccacg tctccgagcg
      841 cgcccgccgc cctgttcgag agcccgcccg ggttcgtggt cgtgcccgcc acctggagcg
      901 cgctgtcggc cgccccttca gccctcgtcc ttcacgtcga gtcccggaaa gtgaggcagc
      961 catggttctg agacgctgga tcgccggcct tttcctgatc gtcctgatgt ccctcatgtc
     1021 tgttagagcc tgggggcagg gtgcgggccc cggcgaggtg agcctcccgc tggcggacta
     1081 cctggccctg gccgggcgtg ccgaggcggc cgaaaaggag cgggcggagc gcgccgccgg
     1141 gcgggaggcg ccggtggccg aggtgacggc gcagcgggtc tccctggtgg ccggcgaaga
     1201 ggaggccgag gtgacctccg agctggaggt cctcgtccag ggtcacccgt ccaagcaggt
     1261 cttcctgccc ctggccggga tcgccgagcg ggtggaggtg acgcccgcga cggcggcgct
     1321 gaccgccacc gaggggggcg ccatgctggt gaccccgggt ccgggaaagt acctcgtgcg
     1381 ggtccacggc cggctgcccg tggcacgcgc cggcggcgtc ggccggttgc cgctggcgcc
     1441 ggtggtggcg ccggtcgccg aggtccaggc cgacctgccg gcggacgcgg ggtggagcag
     1501 ccccgggacc gtggtggtcg aggagcgcga gcaggacggc cgccggcggg tgcgtctcgc
     1561 ggcgcagcgc ggcaagaccg ccgtgctcga gctgcggcgg cgggtcgacg gcagcgacgc
     1621 ggaccgcctc ctcgcccgcg ccgtggtcct cacccttttt cagctccggc ccgacggggc
     1681 gcggcgccac gacgtcgtcc tctacgaggt ggcgcgcggc gggctggcca gcttcgccgt
     1741 ggagctgccg cccgggctcg aggtggagca ggtgggcacc gacgaggggg ccgtggtgcc
     1801 gttggccgag gcgcgccgcc tgaccgtcca tcgccagaag cagctccagg gcgccggcta
     1861 tcttgtgctg acgtcgacgc cgccctcggg tgtgtccacg ggccaggtcc ccttcgcctt
     1921 cgccctgccg ggggtggagg tgcgggcgcg ctacctcgcc ctggccagct cggtggccgc
     1981 cgacgtccag ccggtccccg agaagagctg ggcgcgggtg gacctcgacg acctgccggc
     2041 cgagctgcgg ggagcgctgg gaggccgcgg cctctcggcg gcttggcggc tgctcgctcc
     2101 gcccgcggcg ccggcggcat cggcgggcct ggccctggcg gtggcggagc tgccgaaggc
     2161 gccggagctg gaggaggtgg tccgcgcccg ggagacgatg accctggtga cgttggacgg
     2221 cacggtcctc catcgcgacc tcttcacggt ggagggcggg ggcgaggtct tcgatctgac
     2281 gctgccggcg ggggccaccc tctggtcggc gcgggtgggg gagcagccgg tgcggccggt
     2341 ggagcgcggc ggccgtctgt cggtcccgct cggcttccaa tcgggcctcg cgagggtcga
     2401 ggtggtctcg gtgctgcccg gggagatccc ggagggccgg tcgaggctcg ccctcgagct
     2461 ggcgcgggtc gaggcgccgg tgctcgatca ccgttggcgt ctgctgttgc ccgaaggcgc
     2521 ccgctaccgc ttccgggacg gcgacctgct gccggcctgg gagatggtca cgttcgtcac
     2581 caagagcgag gcctggcagt ccctgggtca gggccaggcg ggcgtccgcg gcagggtccg
     2641 cgacgagaag ggcagaccgc tggcgggtgc cacggtcacc ctcctcagtc gcggtctgca
     2701 ggcggagagc accgctgtga ccggggctga tgggggattc gccttcactc tcctcgccgc
     2761 cggggagtat gaaatgacgg cgagctccga aggacaccag ccggaggtct atcgcttcag
     2821 cctcggctcg cgggaagtca ggccggccta catccagcta actaccgggc aggtggtttc
     2881 cgaggagatc ttggtgtcga ctcctccctc tttcgatacc accacgatcg gcgagaactt
     2941 cgattacgaa agcgaggtgg aatcgctggc gatccaggaa agatatttcg agaacgtcgc
     3001 actgttgagc aacggtctgg tgggtggcgt caagccgctg ccggtgacca ttccggaaac
     3061 aggaaaggtt ttgatcctga ccggagtcct cccccccgag cgggtggcgg ccgagttgga
     3121 ggtgcgggcg aagagataag tcgggagggt gcgcctccac ccaagggagg cgcacccagc
     3181 tgcggaagcg ggcactggtc aactgaagga ctcgtcgccc tggacctgca ccaccggcac
     3241 cgccacagtc gccgccactc cctgcgggtc cgccagccgc cggctcaggg cggtgaagca
     3301 taggtcgcgt ggggtgcaga acgcgaactt gagggattcc atagcatcgg cgaccaggct
     3361 cgcggggcgc agctgggccg cctcgcttgg tactggaagg agctcctgga ggggcggcag
     3421 cccctccgag atccccaggg tgatggacag gttgtcccga gataggacgg agacgccccg
     3481 ccccgccagg tgatcggcca gcgtcgcgtt ggccttgagc cccgcgaagg tccaccagtg
     3541 ggctctgcct ggcgcccctc gttccaggga cgtcgcctcc tggctgagcc aggggaagcg
     3601 cccgcggaat tcgtgcagct tctggcgggc acggagggtg agcaacccct cgtgcccctc
     3661 ccccaggagc acccgctttt gcgcttggca gagggcaaag cccaggggga gcccgtcgcc
     3721 cagccagagg gagcgccccg ggatctcgat gggctccacc caggcctccc gccgcgacca
     3781 gtccacgtgc accacccgcc agctacggcc gccgagcagc aggacggtcc ccccgggagt
     3841 ctcgagctgg aaggtggcgt cgtccacgaa gcccagctcc gtccggccgt gcagcacccg
     3901 caccgatggc gccgcgtcga agaccgacag cagctccagg aagcccttgc cccgaaaccg
     3961 gcggtcgctc tccgggccga atccgagcag gcccgagtcc tcgtggagga agccctcgct
     4021 caggaggtga tcgaccaccg accgccgggc gccggccggc aggacggcga acccgggcat
     4081 gggatcgagc caggtggacc acgctccccg tggcatcccc gggagctggt atgccagcgc
     4141 caggagctgc tgtgcataga tatggaaggg cagcgccggc ggctcgaccg gttctacgta
     4201 gccctcgctc cacagaagcc cgatggccgc ggcccgcagc agggtctcgg gagccgtggt
     4261 cagaaacaac atgttgcgtt tcgtaccctg ccgtctccct gtcctcccca gccgctgaag
     4321 gaagctggcg accgtcgccg gagcctcgat ctgaatgacc cggtcgaggt cgcccacgtc
     4381 gatccccagc tccagcgtgc tggtggccac gatgacacaa tccctggcct cggcgaaggc
     4441 ctgctcggcc cgccgccgct cgtcgatgcc cagggagccg tgggagacgt gggtctccac
     4501 ccccagctcc cgaagccgga aggccaggtc ctccacccgt gaccggctgt cacagaagac
     4561 cagccgcttc tcgccgcggt atagccggga cagcaccacc gcggcgttct ccaggcttcc
     4621 cactgcgtca atctggacgt ccgcctccac ggtctgaccc gaatccggag cgaccacggt
     4681 ccgtggtccc ggtgaggagc cggcgagcca ggtcaggagc tggggcggat tgccgatggt
     4741 ggcggacagc ccgatccgct ggaggtcccg gcccgctact cgcgacaccc tctccaggac
     4801 cgacaggagg tgccagccgc ggtcgtcccc cgcgaaggcg tggagctcat ccaccaccac
     4861 ggaccgcagg ttgtagaaca gcccggcgtg atcgatgcct cgggaggtga gcatcacctc
     4921 cagcgactcg ggcgtggtca gcagcaggtc cggcggctca cgctggatct gccgccgagg
     4981 gccctgtcct acgtccccgt gccagagccc ggcgcgccgc cccagtaacc ccagatagcc
     5041 ggagaggcgg acctccaggt tgttgagcag cgcccgcagc gggcagacat agagaaccga
     5101 gagcccctgc cagtcctcgc tcagcatgcg ggagaagagg gggaagacgg ccgcctcggt
     5161 cttccccccg gccgtcggcg ccaccagcag ggcgtgactg cccgccagca ccggctcgat
     5221 ggcgctctcc tggagcggcc gcagggtccg ccagccgagg ctgttgacga tgtggtgctg
     5281 cagggccggg tgcagacggt cgaacgcgtc caccggtcag agctcgaggt cgatgtcgtc
     5341 cgggctcagg gctccccggg cgccctgctc ctcacgggtg agctctccgg gcgccagggt
     5401 gagggcatag tgcgtccggg gatcgaagtc tggatattgc tccacgcgat ccaggacgtc
     5461 ggacaccagc ttccgcagga agagccgcgg ggcgacgccc actttgccac ccaactcgcc
     5521 ggcgaccgct cgggccagat ctgtcagata ggtgtccgag accagcttgc ggacccgctc
     5581 tgcctcgggc gaccccccgg cgaacaggtc gcggacccgc ctccccacct cctccagccc
     5641 ctggaggtcg aagttgcgta gccggatctg caccgcccgg gggttgtcga accgcgcctc
     5701 ggtggtgaag tccacctgga ggcgctgagc caatccgggc gaaccccgaa gagtctccaa
     5761 attttcccgc ttccgcctct tgactgccaa tcccacctct tctatgcttc tgtcattcgg
     5821 cagtacccgc aggacacgga aggcgcgccc cgcgatgcta tgaagtggaa gggacgcgct
     5881 tcccggcgaa gcagacgaag cagtggtttc ccggctggac cgcggccggc atgaagccgg
     5941 tcgcgtttgc gagacggccc ggggggtgag tttgggcctc atcccccgga cctgaccgcg
     6001 cgaaaggagg tagtcctatg cgcagctttg atgatcgtag cagctcggcc attcacccca
     6061 acgcgttcga cggcccgttc ctgcgccgtc tggcggagcg ggacgagccg cccacggccg
     6121 gcgacgccga cgtggccggc ccgtggcgga tcgaggaaat tccccagcac ggcttcggcc
     6181 tgttccggct gggggagagc ctgtcccgcg gcttcgcccc ggtggcgatt ttcccctccc
     6241 gctggctcgc cctcctcatg gcagccatcc tccccggtac cggccgcgat ccgctggtcc
     6301 gcctggagaa gctgctggat gtggagggcg gctatcccct ggtcctcccc ggcccggacg
     6361 gcggggaggt cgtcggccgc ttccagctct tcgacgaggc ggctgtcgac gccctgaacg
     6421 tggccgtcca tctcctccaa gcgccggagt ccctggcgta cctgttggag gcggcggggg
     6481 ccgtcaccct cgagcgctgc ggcgcgatcc tcgacgagcg gaccgcggca ccgcagtagt
     6541 cgtccttctg gagccgccgg ccgggagctg ttccggccgg tggcgcagtc ttccaggatt
     6601 tcttcactgt cgtctcggag cgtttccctg caggagggaa acgctccgga gcgactcctc
     6661 aacactgagg gttgatttct cagtgttgag ggacgtcgcc gtagtcctga ggaacggctc
     6721 cgtagcgttg aggagcggct cctcagtatt gaggaacgac gccgaagcat tgagggatga
     6781 ccccgaagtg ttgagggacg gccgcggagc attgaggagt cacgccgtag cgttgaggaa
     6841 tggccccgta gtgttgagga atggccccgt agtgttgagg agcggctccg aagtactgag
     6901 gggcggctcc tcaacgttga ggaacggcgc cgtagcattg aggagcggtc cctcagtgtg
     6961 gaggaatgtc ccggaagcat ggaggagcgg ccttggagcg ttgagggata gcgcctcagt
     7021 gttgaggaac ggcgctggag cattgaggga tcgccccgga gcattgagga gcggcactgt
     7081 ttcgcctcag gctcttctgt gggacagaat gtcgccgtgg cccagacgtt ccatgggggg
     7141 ctctccagga gcctcgtcgt ccttgtcgcc ctcgttgcga cggcggcgct aggggcctcc
     7201 tgccgcgccg ccaacgagtc tccggccatc gaggtcctca cgccggggga gacggtgacc
     7261 gcccgcctcg ccgagggggc agtgcacgcc tacgggatca gcgttccagc gggccacctc
     7321 ctgcatctcg tggcggatca ggaaggtgtg gacgtcgtcc tgaccctccg ccgttccacg
     7381 ggtgagcccc tcctcgagat cgatagcccg agcggaagct ggggcccgga agagctctgg
     7441 tggatcgctc cggactccgg cgtctggcgt ctggaggtcc ggccgtggac gcccgctccc
     7501 cgaggtcgct atcagttgcg ggccctggcg gtccggcctg cgagttcgag agacggcctg
     7561 cgggcagccg ccgcgaggaa cgagtgggaa gccgggcgcc tgctggccgc cggcgacgct
     7621 gctgcggccc ggcgcgagct cacggccgcg cgcgagatgt ggcggcgtgc cggcgtgccg
     7681 ctccaggagg ccctcgccgg tctgcggctg gcggcagtct ccgaagcggt ggaagacgcg
     7741 gcgggcgagg tggagtcgta ccggagcgcg ctgacgggtc tccgccgtct gggccaaccc
     7801 cgcctggagg tcttctccct gctgcgtctc ggcgaggcgc tgcgcaaggc cggggatctc
     7861 gccggcgcct gggaggctca ggaggaggcc ctggggctcg cccagcgccg gggtctgaag
     7921 gaggaagcgg ccttcgcgct caacaacctg gccttgatcc tcgaagcgcg gggggagacc
     7981 ggggccgcgc gggaacgcta tcgccacacc gccgccctgt tccggcgcct gggcctgaag
     8041 gccgaggagg ccatggccct gcacagcctg gggacctgct cgagcctgct cggccgcctg
     8101 aaagaggcgg aggcggcgct gaccgcggcc ctggcactcc ggcgctccct cggcgacgtg
     8161 cgcggcgagg gagcgacgtt gaccgagctg ggatgggtct accacctccg cgctctggcc
     8221 gggggtgggg agaccgcccg ggagaaggct cgcggtttcc tgcaggaggc cctgctccgc
     8281 cggcgtgagg cccgggactc gctcggcgag tccgggaccc tcgaccgcct cggaacggtc
     8341 taccgccact ccggcctctg gaacgaggcc ctcgcctgct accagcggtc cctcgttctg
     8401 atccgggaca cgccgcccgg gcgccggcag gcgaataccc tctccaatct ggcggagcta
     8461 tggctcggcc ggggccggcc cgcgaaggcc cgcgagctgg ccacggaggc ggtggcccgc
     8521 ttcgccgccc tgcccgtccg ggacgcccat ggcgagatcc acgcgcgcta cctcctcggc
     8581 cgcgccgccg ccgggctggg cgatctcgcg ggcgcccggc gcgagctgga acaggtcctc
     8641 tctacggtgg aggcgctgcg ctcggagctg ggcgaccaga cgctgaccct ggccttcttc
     8701 gcccagcggc agctctactt cgagggggcc atcgagaccc tgatgaacct cgatgccctc
     8761 tacccgggtc aggggttcga catcgccgcc ctggagacca gcgagcgagc ccggatgcgc
     8821 accctgctcg acagcctcgc cgcccggcgc cgccgcgcgc aggggaaaga ggagagcgac
     8881 gaggcccggc cgatgacggc ggagcgcatc cagcgggagc tgctggatgg cgacacggtc
     8941 ctgctcgagg tggccctggg agaggagcgg ggcttcctct gggtggtaag ccgcgagcgg
     9001 gtggaggcgt tccgggtgcc ggggtggcga gtcctccggc cgctggtcga ggaggcctac
     9061 cgggggctcc gcggcggcgg cggccgggaa gcgtcgcggc agctcgtgga gcttctcctg
     9121 gggccggcgg tggaccggct ggacggtggg ctcgcaacgc ggcggctggc ggtggtcacc
     9181 gacggcctcc tggctcacat ccccttctct gccctgccct ggggggaaaa ggggcggccc
     9241 ctggtggaga gctacgagat cgtccgcctg ccctcggcga cggcgctggc catgttgcga
     9301 caacgggcgg cgctccggcc ggccgccgag gccggcgcga cggcggtggt ggcggacccc
     9361 gttttctcca ccttggaccc gcgggtgcat cgtgccggcg gcgctccgcc gg
//
LOCUS       DASPJI010000478         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_714503, whole genome shotgun sequence.
ACCESSION   DASPJI010000478 DASPJI010000000
VERSION     DASPJI010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714503"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VEL74_13070"
     CDS             complement(<1..936)
                     /locus_tag="VEL74_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63506.1"
                     /translation="MKTLQSFLATVTALLAVSLPLAAAEAPAETPQPELPRGQLIEKV
                     TAQEAPDQTYALYLPSSYRPDRTWPVLYAMEARGQGADFARRFTGAAEKLGVIVASSY
                     NTASDGAMQPNLTAMRAMWADTHRRLSIDDKRVYAAGFSGTVRAACLLASLSPGSIVG
                     VIGASGGFPFDRPPSKDTPFVFFGTVGDRDFNYDELMDLEADLSKAGIAYRIELFEGG
                     HEWPPADLAGEGLAWLELQSAKAGRRVQDSDLLDGVWKQGMERARAHESGGRLPEAFR
                     GYQALAADFRGLPPRPELTELRAKIEAMERSEALAT"
     gene            1179..1538
                     /locus_tag="VEL74_13075"
     CDS             1179..1538
                     /locus_tag="VEL74_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63507.1"
                     /translation="MRTQTRFLSLVPPEEEVAVLRRPRGVQPVDLDETLPIVAKRPAL
                     QPDTLPMKCLRCNGPVERGTAPVSVQRNGYRIAWESVPAWVCTRCELPYFEPREVERV
                     RTAVKALRAMGPRRVAP"
     gene            1678..>3028
                     /locus_tag="VEL74_13080"
     CDS             1678..>3028
                     /locus_tag="VEL74_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYG63508.1"
                     /translation="MSQQRDDVRNLAIIAHVDHGKTTLVDAMLWQSGIFRENEHVVDR
                     VMDSIDLEREKGITIMAKNTAINFRGTRINIVDTPGHADFGGEVERTLKMVDGVLLLV
                     DASEGPLPQTRFVLRKALEDNLRPIVVVNKIDRPDARIAEVLNEVYDLFIDLDATEEQ
                     LEFPVLYTNARKGTCRAEPDAPDQTLEPLFEEIIRTVPAPVYEAEMPLQFQVITLDYD
                     DYVGRLAIGRVFNGRVRKGQEAGLCRLDGTVDPVKISVLYGYEGLRRVEIAEAGPGDI
                     VALAGIEEVNIGETLSERDNPQALPPIQIDEPTISMVISINDSPFSGRDGKHVTSRKL
                     KERLDREVLGNVAIRVEPTDSPEAFKVSGRGELQLAILIEMMRREGYEMSVGKPEVIT
                     RRIDGVLHEPMEMLIIDCPEEFIGVVTEAMGRRKGQMAKMVNHGTGRVRLEFETPSRG
                     "
BASE COUNT          473 a         1050 c         1032 g          473 t
ORIGIN      
        1 cgtcgccagc gcctccgatc tctccatggc ctcgatcttg gcccgcagct ccgtcagctc
       61 cggccgcggg ggcaagcccc ggaagtccgc ggccagcgcc tgatagcccc ggaaggcttc
      121 gggcagccgg ccgccggact cgtgggcgcg ggcgcgctcc atcccctgtt tccacacccc
      181 gtccaggagg tccgagtcct ggactctacg ccccgccttg gccgactgga gctccagcca
      241 ggccagcccc tcgcccgcga ggtccgccgg cggccactcg tgcccacctt cgaagagctc
      301 gatccggtag gcgatcccgg ccttggacag gtccgcctcc agatccatca gctcgtcgta
      361 gttgaagtcc cggtcgccca cggtgccgaa gaagacgaag ggggtgtcct tggagggcgg
      421 ccggtcgaag gggaagccgc cgctggcgcc gatgacgccg acgatgctgc cgggggagag
      481 ggaggccagg aggcaggcgg cccgcaccgt gcccgagaag cccgcggcat agacccgctt
      541 gtcgtcgatg gagaggcggc ggtgggtgtc ggcccacatc gcccgcatgg cggtgaggtt
      601 cggctgcatg gcgccgtcgc tcgccgtgtt gtaggaactg gcaacgatca ctcccagctt
      661 ttcagcggcg cctgtgaagc gccgggcgaa atccgccccc tggccccgcg cctccatcgc
      721 gtagaggacc ggccaggtgc ggtccggccg gtagcttgag gggaggtaga gggcataggt
      781 ctggtccggc gcctcctgcg cggtcacctt ctcgatcaac tggccccgcg gcagctcggg
      841 ctgcggcgtt tcggcgggtg cctcggcggc ggcgaggggg aggctgacgg ccagcaacgc
      901 cgtcacggtc gcgagaaagc tctgcaaggt cttcacgggt cgctccttcg ggccgcaatg
      961 atcccacacc gggaaggtga acacggagcg acacggacga tcacggactg acatggacag
     1021 ctctctgctc cgtccgtccg tgaaggtccg tgttcgtctg tgctcgtccg tgtccctccc
     1081 cggaacaatc gccccgcttt tcctgttctt cctggtggga ggctcttccc gggcaggaga
     1141 tttgcattct cttctggccg tcaggagagg agagcctcat gagaacgcag acccggttcc
     1201 tgtccctggt tccgcccgag gaggaggtcg cggtccttcg ccggccgcgc ggggtccagc
     1261 ccgtcgatct cgacgagacg ctgcccatcg tggccaagcg cccggccctc cagccggaca
     1321 cgctgccgat gaagtgcctg cgttgcaacg gcccggtgga acgcggcacg gcaccggtct
     1381 cggtgcagcg caacggctac cggattgcct gggagtcggt gcccgcctgg gtctgcaccc
     1441 gttgtgagct gccctatttc gagccgcgcg aggttgagag ggtgcgcacc gccgtcaagg
     1501 ccctgcgggc tatggggccc cgccgggtcg ccccctagag tccgctcccc tctggccctt
     1561 tcagcgctct gtgctatcct gccgccccgc ggcggcagga ctgccccagc tcggcccact
     1621 gggcggagcc gcgcctttcc ccggcttttc gatcccccgg atcagtgagg atatcccttg
     1681 agtcagcaga gagacgacgt ccgcaatctg gccatcatcg cccacgtcga ccacggcaag
     1741 accacgctgg tggacgcgat gctgtggcag agcggcatct tccgtgagaa cgagcacgtg
     1801 gtggaccggg tgatggattc catcgacctg gagcgcgaga agggcatcac catcatggcc
     1861 aagaacacgg ccatcaactt ccgcggcacc cggatcaata tcgtcgacac ccccggccac
     1921 gccgacttcg gcggcgaggt cgagcgcacg ctcaagatgg tggacggcgt gctcctgctg
     1981 gtcgacgcca gcgagggccc cctgccgcag acccgcttcg tgctgcggaa ggcgctggag
     2041 gacaacctcc ggcccatcgt ggtggtcaac aagatcgacc gcccggacgc ccgcatcgcc
     2101 gaggtgctga acgaggtcta cgacctgttc atcgacctcg acgccaccga ggagcagctc
     2161 gagttccccg tcctctatac caacgcccgc aaggggacct gccgggcgga gccggacgct
     2221 cccgaccaga ccctggagcc gctgttcgag gagatcatcc ggaccgtgcc ggcgccggtc
     2281 tacgaggccg agatgccgct gcagttccag gtcatcaccc tggactacga cgactacgtc
     2341 ggccgcctgg ccatcggtcg ggtgttcaac ggccgggtgc gcaaggggca ggaggcgggg
     2401 ctctgccggc tcgacggtac ggtcgatccg gtcaagatct cggtcctcta cggctacgag
     2461 gggctgcgcc gggtcgagat cgccgaggcg ggcccgggcg acatcgtggc gctggcgggc
     2521 atcgaggagg tcaacatcgg cgagacgctg tccgagcggg acaaccccca ggcgctgccg
     2581 ccgatccaga tcgacgagcc caccatctcc atggtgatct cgatcaacga ctccccgttc
     2641 tccggccgcg acggcaagca cgtcacctcc cgcaagctca aggagcgcct cgaccgcgag
     2701 gtgctgggca acgtggcgat ccgggtcgag cctaccgact cccccgaggc tttcaaggtg
     2761 tcgggccgcg gcgagctgca gctcgccatc ctcatcgaga tgatgcggcg cgagggctac
     2821 gagatgtcgg tcggcaagcc cgaggtcatc acccgccgca tcgacggcgt gctccatgag
     2881 cccatggaga tgctgatcat cgactgcccc gaggagttca tcggcgtcgt cacggaggcg
     2941 atggggcggc gcaaggggca gatggcgaag atggtcaacc acgggacggg gcgcgtccgc
     3001 ctggagttcg agacgccgtc gcgcggcc
//
LOCUS       DASPJI010000479         3208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_714533, whole genome shotgun sequence.
ACCESSION   DASPJI010000479 DASPJI010000000
VERSION     DASPJI010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714533"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3208
                     /locus_tag="VEL74_13085"
     CDS             <1..>3208
                     /locus_tag="VEL74_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG63509.1"
                     /translation="GPTETVVGCCVYEVPSGARLTGTVPIGRPIARTRIHLLDTSLEP
                     VFPGTRGELFIGGAGVARGYLGRPDLTADRFRPDPFSPSPGERLYKTGDLGRYLPGGD
                     IEFLGRTDHQVKVRGFRIELGEVEAVLASHQAVRDCVVLAPLDSSGARRLVAYVVGPE
                     PPNTEELRAFLAGRLPEPMVPTAFRALDALPLTLNGKVDREALPAPDPGLLAATGSYA
                     APADPAEELLAGIWSEVLGLDAARVGVCDDFFALGGHSLLATQVASRIRKAFGVELPL
                     RLVFEARTVRDLARVVQEVRRADAPQAPPLVPVPRKGALPLSFAQQRLWFLDQLEPDS
                     PLYNVPVALRVEGYLGIVLLAKSLAALAQRHEALRTVFPAVEGSPMQVIRDTPDVQLP
                     LIDLSGLPGAGLGELVRRLATEEAGRPFRLADGPLLRATVLRLGPQDHAVLLTLHHIV
                     SDAWSMGVLVRELTALYEAYGAGRATPLRELPALPVQYADFASWQRGWLQGETLEREI
                     AYWRGQLAGLPPLLELPVDHPRPAVRSLRGAALPVRLPADLAPRVRAFSRQEGATPFM
                     VLLATFQGLLSRYSGQPDLAVGTPIAGRNHLETEGLIGFFVNTLVLRLRADLSEGPSF
                     RELSGRVRETALAAHTHQDVPFEKLVEELAPDRSLSHTPLFQAVFSLQNTPSETPALA
                     GARLSWLAAPTSTAKFDLTLALGEREGALEGSIEYSTDLFEAVTIERLALHFARLLAA
                     ALEEPGAGVGELPLLTDAERAELVAAWSPTTAWREETTLPELFREQVERRGETEALSF
                     EDERLSYRELGRRVRSLANHLKGMGVGPEVRVGLCAERSAHLVVGLLAILEAGGVYVP
                     LDPAYPAERLGFLMADSGVAVVVGQEAVRDRLPGGTWKWVPLEREPAPADGTAVPPLR
                     LMPDAAAYVIYTSGSTGRPKGAVVSHRNVVRLLRSSEADFGFGPGDVWTLFHSFAFDF
                     SVWELWGALAYGGRLVVVPYWVARSPQAFAGLLEQEGVTVLNQTPSAFRQLMRVEGEP
                     ALRLRILVFGSEALDPSNLRGFHQRYPAVRLVNMYGITE"
BASE COUNT          394 a         1095 c         1205 g          514 t
ORIGIN      
        1 cggtcccacc gagacggtcg tcggctgctg cgtctacgag gtgccctccg gagcacgtct
       61 taccgggacg gtgcccatcg gccgcccgat tgcccgtact cgcatccatc tgctggacac
      121 gagcctggag ccggtgttcc caggtacgcg gggtgagttg ttcatcggcg gggcaggggt
      181 ggcccggggc tatctggggc ggcctgacct cacggccgac cgcttccgtc ccgacccgtt
      241 cagcccgtct ccgggagagc gactgtacaa gacgggcgat ctcgggcggt atctgccggg
      301 cggtgacatc gagttcctgg ggcggacgga tcaccaggtc aaggtgcgag gattccggat
      361 cgagctggga gaggtcgagg cggttctggc gagccaccag gcggtgcgcg actgcgtggt
      421 gctagccccc ctcgactcct ccggcgcacg ccgtctcgtg gcctacgtcg tggggccgga
      481 gccgccgaac accgaagaac tgcgcgcctt cctcgccggg aggttgccgg agcccatggt
      541 gcccacggcc ttccgcgcgc tggatgccct gccgctcaca ctcaatggca aggtggaccg
      601 cgaggcgctg ccggcgccgg accccggcct gctcgccgcg acggggagct acgcggcgcc
      661 cgccgatcca gccgaggagc tgctggccgg gatctggagc gaggtgctgg ggctcgacgc
      721 tgccagggtg ggagtctgcg acgacttctt cgcgttgggc ggccattcgc tgctggcgac
      781 ccaggtggcc tcccgcatcc gcaaggcttt cggtgtggag ctgcccctgc gcctggtctt
      841 cgaggcgcgg acggtgcggg acctcgcccg ggtcgtccag gaggtgcgcc gggcggacgc
      901 tccccaggcg ccgcctctcg ttccggtgcc caggaagggc gccctcccgc tgtccttcgc
      961 gcagcagcgg ctctggttcc tcgaccagct cgagccggac agcccgctct acaacgtgcc
     1021 ggtcgccttg cgggtcgaag ggtacctcgg catcgtcctg ctcgccaaga gcctggccgc
     1081 gctggcccag cggcacgagg ccctgcgcac ggtcttcccg gcggtcgagg gctcaccgat
     1141 gcaggtgatc cgtgacaccc ccgacgtcca actgcctctg atcgacctct cgggactgcc
     1201 gggagccggg ctcggcgagc tggttcgccg cctggcaaca gaggaggcgg gccggccgtt
     1261 ccggctggcc gacggcccgc tgctgcgcgc caccgtgctg cggctgggcc cgcaggatca
     1321 cgccgtgctg ctcaccctgc accacatcgt gagcgacgcc tggtccatgg gcgtcctggt
     1381 gcgggagctg acggccctgt atgaggcgta cggggctggc cgagcgaccc cgttgcggga
     1441 attaccggcg ttgccggtgc agtacgcgga cttcgcctcc tggcagcgcg gatggctgca
     1501 gggcgagacg ctggagaggg agatcgccta ctggcgcggc cagctcgcgg gcctcccgcc
     1561 gctgctggag ctgccggtgg accacccgcg gccggccgtg cggagcctgc gcggtgcggc
     1621 actgccggtg cggctgccgg cggacctcgc tccacgggtg cgggcgttca gccggcaaga
     1681 gggggccacg ccgttcatgg tcctgctggc caccttccag gggctgctct cgcgctacag
     1741 cggccagccg gacctggcgg tcggcacgcc catcgcgggc cgcaatcatc tggagaccga
     1801 ggggctgatc ggcttcttcg tcaacaccct ggtgctgcgg ctgcgggccg acctttcgga
     1861 ggggccgtcg tttcgcgagc tgtcaggccg ggtccgggag acggctctgg cggcgcacac
     1921 ccatcaggac gtgccgttcg agaagctggt ggaggagctg gcgccggacc gcagtctcag
     1981 ccacacaccg ctgttccagg ccgttttcag tctgcagaac accccctccg agacaccggc
     2041 actcgccggt gcccggttgt cctggctggc cgccccgacg agcacggcca agttcgacct
     2101 cacgctggct ctgggggagc gggagggcgc tctcgagggt tcgatcgagt acagcaccga
     2161 tctgttcgag gcggtgacga tcgaacgcct ggcgctccac tttgcgcgcc tgctcgcggc
     2221 ggccttggag gagcccggcg ccggtgtcgg agagctgccg ctgctcaccg acgccgaacg
     2281 cgcggagctg gtcgccgcct ggagccccac cactgcatgg cgagaggaga cgaccttgcc
     2341 ggagctgttc cgcgagcagg tggagcggcg tggcgagacc gaggcactgt cgttcgagga
     2401 cgagcggctg agctatcgcg agctgggccg gcgggtgcgg tctctggcca accacctgaa
     2461 ggggatgggg gtcggccccg aggttcgggt ggggctgtgc gccgagcgtt cggcgcacct
     2521 ggtggtgggc ctcctggcga tcctcgaggc cggcggcgtc tacgtgccgt tggaccccgc
     2581 ctacccggcg gagcgtctgg gcttcctgat ggcggactcc ggggtggccg tcgtggtcgg
     2641 gcaggaggcg gtccgggacc ggctgcccgg gggcacctgg aaatgggtgc cgctggagcg
     2701 ggagccggcc ccggcggacg gcacggcggt cccgccgcta cggctgatgc cggacgcggc
     2761 ggcgtacgtg atctatacgt cgggctcgac cggccgcccc aagggggcgg tggtgagtca
     2821 tcgcaacgtg gtgcggctgc tgcgctccag cgaagcggac ttcggtttcg gaccggggga
     2881 cgtctggacc ctgttccact ccttcgcctt tgacttttcg gtctgggagc tgtggggggc
     2941 gctggcctat ggcggccggc tggtggtggt gccgtactgg gtcgcccgct cgccccaggc
     3001 gttcgccggg ctgctggagc aggagggcgt cacggtgctc aaccagacgc cctcggcgtt
     3061 ccgccagctc atgcgagtgg agggggagcc cgccctccgc ctgcgtatct tggtgttcgg
     3121 gagcgaggcg ctggacccct ccaacctgcg cggcttccac cagcgctacc cggcggtgcg
     3181 gctggtgaac atgtacggca tcaccgag
//
LOCUS       DASPJI010000480         3087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_27329, whole genome shotgun sequence.
ACCESSION   DASPJI010000480 DASPJI010000000
VERSION     DASPJI010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27329"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3087
                     /locus_tag="VEL74_13090"
     CDS             <1..>3087
                     /locus_tag="VEL74_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63510.1"
                     /translation="RTWIEEVLAGIWSDVLGVERVGTEDDFFALGGHSLLVTRVVSRL
                     RGAFGIELPLRTLFEAPTLAGLAGRVELALGAEPGVAAPPLVPVPRGGALPLSFAQQR
                     LWFLDQLEPGSAAYNLPSAVRLTGELSPALLARVFTEIVRRHESLRTTFTARQDGPVQ
                     EIAPPGPVELPVIDLSGLAGAAREAEARRQAGEEARRPFDLQRGPLLRLRLLRLAPDE
                     HALLMTMHHIVSDGWSAGVLLGEIATLHTAFAAGRPSPLPELPIQYADFAAWQRGWLR
                     GEVLEAQAAAWTQRLAGAPRVLELPLDRPRPAVQAHRGSSLAVSLPPELSAAVRELCR
                     REGATPFMALLAGWAALLGRHAGQDDVLVGTPIAGRNRREVEGLIGFFVNTLVLRTGL
                     SGEPGFGELLARVRQACLEAYTHQDLPFERLVEELVPGRDLSVSPLFQVMLVLQSETP
                     RLSVPGLELSPLPAEGRVAKFDLTLALEETAGVFAGALEYDRDLFDRSTVARLSARFA
                     AFLEGALAAPDAGLAELPLLLPGERHQVLREWNDSATSYAEGRCLHELIAARAAATPD
                     AVAAVFEDEALTCHELARRAHRLARHLAGLGVRPDGRVGVLMERSLEMIVGLAGVLAA
                     GAAYVPLDPSYPAARLAVLLESAGVDVVLAQSRLAPLLPRRGERGEKVVALDGPPSRE
                     AAGSPGRAGEDNLAYVIYTSGSTGTPKGVMIPHRGIVNRLLWMRQTYGLAPADRVLQK
                     TPFSFDVSVWELFCPLITGSRLVLARPEGHKEPAYLADLIAREGITTLHFVPSMLQAF
                     LEVPGLEALGSLRRVMASGEALPPDLVRRFFRRLGGVELHNLYGPTEASVEVSAWAGE
                     PEPPRGVVPIGRPIANLRLHVVDRGLRPQPVGVAGELLLGGVGLARGYLGRPELTAAA
                     FVPDPLGGGPGERLYRTGDLARTLPDGQVEYLGRIDHQVKIRGVRIELGEVEAVLGSH
                     PAVRECVVAARGEGAGSPRLVAYIVASSGTDPVALRAFLAAKLPEAMVPAAFVILDA"
BASE COUNT          310 a         1096 c         1238 g          443 t
ORIGIN      
        1 gcggacctgg atcgaagagg tgctggcggg gatctggagc gacgtcctcg gggtggagcg
       61 ggtagggacg gaggacgact tcttcgccct cggcgggcac tcgctgctgg tgacgcgggt
      121 ggtgtcgcgc ctgcgggggg cgttcgggat cgagctgccg ctgcgcacgc tgttcgaggc
      181 gccgacgctg gccgggctgg ctggccgggt ggagctggcc ctgggcgcgg agccaggggt
      241 ggcggcaccg ccgctcgtgc cggtcccgcg cggcggcgcc ctgccgctgt cgttcgccca
      301 gcagcggctc tggttcctgg accagctcga gccggggagc gccgcctaca acctcccctc
      361 cgccgtccgc ctgaccgggg agctgtcgcc ggcgctgctg gcgcgggtct tcaccgagat
      421 cgtgcggcgg cacgagtcgt tgcggaccac gttcacggcg cggcaggacg gcccggtgca
      481 ggagatcgcc ccgcccgggc cggtggagct gccggtgatc gatctttcgg gcctcgccgg
      541 ggcggcgcgg gaggccgagg cccgccgcca agccggcgag gaggcgcgcc ggccgttcga
      601 tctgcagcgc gggccgctgc tgcggctgcg cctgctgcgg ctggcgccgg acgaacacgc
      661 gctgctgatg acgatgcatc acatcgtctc ggacggctgg tcggcggggg tgctgctggg
      721 ggagatcgcc acgctccaca ccgccttcgc ggcggggcgg ccgtcgcctc tgccggagct
      781 gcccatccag tacgccgact tcgccgcctg gcagcgcggc tggctgcgcg gcgaggtgct
      841 ggaggcccag gccgcggcct ggacgcaacg gctcgccggg gcgccgcggg tgctggagct
      901 gccgctggac cggccgcgcc cggcggtcca ggcccaccgc ggctcgtcgc tggccgtgag
      961 cctgccgccg gagctgtcgg cggcggtgcg cgagctctgc cggcgcgagg gggcgacgcc
     1021 gttcatggcg ctgctcgcgg gctgggcggc gctgctcggc cgccacgccg ggcaggacga
     1081 cgtgctggtg ggcactccca tcgccggccg caaccggcgc gaggtcgagg ggctcatcgg
     1141 cttcttcgtc aacaccctgg tcctgcgcac cggcctgtcg ggggagccgg gcttcggcga
     1201 gctgctggcg cgggtccgcc aagcctgcct cgaggcctac acgcaccagg acctgccgtt
     1261 cgagcggctg gtcgaggagc tggtgcccgg gcgggacctc tccgtctcgc cgctgttcca
     1321 ggtgatgctc gtgctgcaga gcgagacccc gcgcctgtcg gtgccggggc tcgagctctc
     1381 ccccctgccg gcggaggggc gggtggcgaa gttcgacctc accctcgccc tcgaggagac
     1441 ggcgggggtg ttcgccggcg ctctcgagta cgaccgcgac ctcttcgacc gcagcacggt
     1501 ggcgcgcctg tccgcccgct tcgcggcctt tctggagggc gccctcgcgg ctccggacgc
     1561 cgggctggcg gagctgccgc tgctccttcc gggcgagcgc caccaggtgc tccgcgagtg
     1621 gaacgactcc gccacgtctt atgcggaggg gcgctgcctg cacgagctga tcgccgcgcg
     1681 ggcggcggcg acgccggacg cggtggccgc agtgttcgag gacgaggcgc tgacctgcca
     1741 cgagctcgcg cggcgcgccc accgtctggc ccggcacctc gccgggctgg gcgtgcggcc
     1801 ggacggccgg gtgggcgtcc tgatggagcg ctcgctggag atgatcgtcg gcctcgccgg
     1861 cgtgctcgcg gcgggggcgg cctacgtccc cctggacccc tcgtatccgg ccgcgcgcct
     1921 cgccgtgctg ctggagagcg ccggggtgga cgtggtcctc gcccagtcac ggctggcgcc
     1981 gctgctcccc cgccgcggtg agcgcggcga gaaggtggtg gcgctggacg gtccgccgag
     2041 ccgggaggcg gccggctctc cggggcgcgc cggcgaggac aacctcgcct atgtgatcta
     2101 cacgtccggc tccaccggca cccccaaggg ggtgatgatc ccgcaccgcg gcatcgtcaa
     2161 ccggctgctg tggatgcggc agacgtacgg gctggcgccg gcggaccggg tgctccagaa
     2221 gacgccgttc agcttcgacg tctcggtgtg ggagctgttc tgcccgctga tcacgggctc
     2281 ccggctggtg ctggcgcggc cggaggggca caaggagccg gcctatctgg cggacctgat
     2341 cgcccgcgag gggatcacca ccctgcactt cgtcccctcg atgctccagg ccttcctgga
     2401 ggtgccgggg ctggaggcgc tcggctcgct gcggcgcgtg atggcgagcg gcgaggcgct
     2461 gccgccggac ctggtgcgcc gcttcttccg acggctgggc ggcgtggagc tgcacaacct
     2521 ctacggcccc accgaggcgt cggtggaggt gagcgcgtgg gcgggcgagc cggagccgcc
     2581 gcggggtgtc gtccccatcg gccgtcccat cgccaacctc cggctccatg tggtggaccg
     2641 cggcctccgg ccgcagcccg tgggcgtggc cggcgagctg ctcctggggg gcgtgggcct
     2701 ggcgcggggg tacctgggac ggccggagct gactgcggcc gccttcgtcc ccgatccgct
     2761 cggcggcggg ccgggggagc gcctctaccg caccggcgac ctggcgcgga cgctgccgga
     2821 cggccaggtg gagtacctgg ggcgcatcga tcaccaggtg aagatccgcg gcgtccgcat
     2881 cgagctcggg gaggtcgagg cggtgctggg gagccacccg gcggtgcggg agtgcgtcgt
     2941 ggcggcccgc ggggaggggg ccggctctcc ccgcctcgtg gcctacatcg tcgcgagctc
     3001 cggcaccgat ccggtcgcgt tgcgggcgtt cctggctgcg aagctgcccg aggcgatggt
     3061 gcccgcggcg ttcgtgatcc tcgacgc
//
LOCUS       DASPJI010000481         4536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_27958, whole genome shotgun sequence.
ACCESSION   DASPJI010000481 DASPJI010000000
VERSION     DASPJI010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27958"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4521)
                     /locus_tag="VEL74_13095"
     CDS             complement(<1..4521)
                     /locus_tag="VEL74_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63511.1"
                     /translation="METLGFVATVRETVLEAFANQDLAFDTLVEELRPPRDLSRNPVV
                     QVTMALQNTPTPVEGALRIAPLSSGESQTAKFDLSLTVEEREEGLTLAAEYSVDLFTP
                     STVARLLAHYATLLEGIAMAPDLPLSRLPLLTEAERHQLLAEWNDTAREAAGGPWVHE
                     LVAAQARRQPEAPAMVDGARQVSYGMLDLWARRIAYALRGLGVSPEVRVGVCLERSPE
                     LLASCLGVLQAGGAYVALDPSHPEERLGFQFEDSGMPVLLTRRGRLAELAAKRGIHCL
                     FPHELDVHGVGEELPTPALAPGNLAYVIYTSGSTGRPKAVDLSHGSLLNLVALQARLQ
                     SVQAADRTTLLAGVGYDATILEIWIYLAAGASLYIAPEAVRTSPEELRNWMAQQGITT
                     SFLPTALAEGLLELGWDDTGEPRMSLRSVLTGGDRLHCFVPESLPFRLINAYGPTEAT
                     VMATTGPVSAMDRPEWAGQVPTIGRPLENVRVHVLEPGGEPRPAPLGVPGELLIGGAG
                     LARGYLGRPDLTAERFVPDPLAASPGERLYRTGDLVRWSPEGEIEFLGRIDHQVKIRG
                     QRIELGEIEAVLTLHPAVREATVLALDGRLAAFLILGAPVASVELREHARRHLPDHMV
                     PTAFVPVTVWPLSTSGKVDRHTLGEIGRQALAQGGESGAESDTLVTPVQELLAALWRE
                     LLEIGHVGLGDNFFDLGGHSLKVAQLRSRVRDAFDLDLPMQCFFEAQTLESQAEVIDL
                     TLRQGGEAGAAIVPVQRDGDPVLSFAQQRLWFLDQLEPGTQLYNIPLVGALEGELDVA
                     ALERSFGEIVRRHESLRTTFPETATGAVQKIASPDAVHVPLPVIDASGLPPAVREREL
                     ERLTREAVRRPFDLARGPLMRITLLRGGEREHLLLICMHHIISDGWSTGVIVRELSAF
                     YRGFVSGSPVPLPPPPLQYADFAVWQRRWLTGEVLEKQMGYWRRQLSGLSVIELPTDR
                     PRPAVETFRGSLLQVALPPALTGAMRELARNAGGTLYMVLLAAFDVLVASYTGRTDIA
                     VGAAVANRNRRETEDVIGFFVNTLVMRTRLSGDPSMHELLGRVRETTLAAYVHEDLPF
                     ERIVEELQPQRDLSRNPLFQVMLVLQNIPLPPLDLPGLSAASLPVNLGTVKFDMALVC
                     WEEEGRLSGQLEYSTTLFDATTMRRLLGHYEAVLARAVANPELPISALAGPTEAERHQ
                     FVEEWNDTRIEFGPGRCIHEWVEEQVAATPEALALIAGDVRLTYRELNAMANRLAHHL
                     RRLGIGPDILVGICVDRTWTMVISLLGVLKAGGAALALDPTYPEERLRYVIEDAGLAL
                     LLAEEKLLPLLPDTGIPRLRIDTDWSLITAGESDANPVCRTHPESTMYLIYTSGSTGL
                     PKGVSFPHRAFVNLLDWQFGHSRLTRHARTVQFATFGFCVSFLEIFAVLCSGSTLVMV
                     PEEYRRDMEALWKHLEEHEVERLHLPFAALKQLADVCGEENRVPRRLREVVTSGEQLQ
                     VGRSIRSLFTNLE"
BASE COUNT          710 a         1582 c         1612 g          632 t
ORIGIN      
        1 ctcgaggttg gtgaacaggc tgcggatgga gcggccgact tggagctgct cacccgaggt
       61 gacgacctcg cgcaggcggc gcggcacccg gttctcctcg ccacagacgt cggcgagctg
      121 cttgagcgcc gcgaagggca ggtgtagccg ctccacctcg tgctcctcca ggtgcttcca
      181 gagcgcctcc atgtcgcgcc ggtattcctc cggcaccatg accagtgtgc tgcccgagca
      241 gagcacggcg aagatctcca ggaagctgac gcagaacccg aaggtggcga actgcacggt
      301 gcgcgcgtgg cgcgtcagcc gcgagtggcc gaactgccag tcgaggaggt tgacgaaggc
      361 gcggtgcggg aagctgaccc ccttgggcag accggtggag ccggaggtgt agatgagata
      421 catcgtcgac tccgggtgcg tccggcagac cgggttcgcg tcgctctcgc cggcggtgat
      481 cagcgaccaa tcggtgtcga tgcgcaggcg ggggatgccg gtgtcgggca gcagcggcag
      541 cagcttctcc tcggccagca gcagcgccag gccggcgtcc tcgatgacgt agcgcaggcg
      601 ctcctcgggg taggtagggt cgagggccag ggcggcgccg ccggccttga gcacgcccag
      661 caggctgatg accatggtcc aagtgcggtc gacgcagatt cccaccagga tgtcgggccc
      721 gatccccagc cggcgcaggt gatgcgccag ccggttcgcc atggcgttga gctcgcgata
      781 ggtgagccgg acgtcgccag cgatgagggc gagggcctcg ggcgtcgccg ccacctgctc
      841 ttccacccat tcgtggatgc agcgtcccgg cccgaactcg atccgggtgt cgttccactc
      901 ctccacgaac tggtgccgct cggcttcggt ggggccggcc agggccgaga tcggcagctc
      961 ggggttcgcc accgcccgcg ccagcaccgc ctcgtagtgg ccgagcaggc gccgcatcgt
     1021 tgtggcgtcg aacagtgtgg tgctgtattc gagctggccg gacagccgcc cctcctcctc
     1081 ccagcagacc agcgccatgt cgaacttcac cgtccccagg ttcaccggca gggaggccgc
     1141 cgagaggccg ggcaggtcaa ggggtggcag cggaatgttc tgcagcacca gcatcacctg
     1201 gaagagcggg ttgcggctca ggtcgcgctg cggctggagc tcttcgacga tgcgctcgaa
     1261 gggcaggtcc tcgtgcacgt aggcggcgag ggtggtctcc cgcacccgcc ccagcaactc
     1321 gtgcatggac gggtcgcccg acaggcgcgt gcgcatcacc agggtgttga cgaagaaacc
     1381 gatgacgtcc tcggtctcgc ggcggttgcg gttggccacc gccgcgccca cggcgatgtc
     1441 ggtgcgtccg gtgtaggagg ccaccagcac gtcgaaggcg gcgagcagga ccatatagag
     1501 ggtgccgccg gcgttgcggg ccagctcccg catggcgccg gtcagcgccg gaggcagcgc
     1561 cacctgcagg agggagccgc ggaacgtttc caccgccggc cgcggccggt cggtgggcag
     1621 ctcgatcacc gacaggccgg agagctggcg gcgccagtag cccatctgct tctcgagcac
     1681 ctcgccggtc agccagcggc gctgccagac ggcgaagtcg gcgtactgca gcggcggcgg
     1741 gggcaggggg accggcgagc cggagacgaa accccggtag aacgccgata gctcacgcac
     1801 gatcaccccc gtcgaccacc cgtcgctgat gatgtggtgc atgcagatga gcagcaggtg
     1861 ctcccgctcg ccgccgcgca gtagggtgat gcgcatcagc ggcccgcgcg cgagatcgaa
     1921 tgggcgccgc accgcctccc gcgtcagccg ctcgagctcg cgctcgcgga ccgccggcgg
     1981 caggccggag gcgtcgatca ccggcagcgg cacgtgcacc gcgtccggcg aggcgatctt
     2041 ctgcaccgcg ccggtggccg tctccgggaa ggtggtgcgc aacgactcgt ggcggcggac
     2101 gatctcgccg aagctgcgct ccagggctgc gacgtcgagc tcgccctcga gcgcccctac
     2161 caaggggatg ttgtagagct gagtccccgg ctcgagctga tcgaggaacc agaggcgctg
     2221 ctgggcgaac gacaggaccg gatctccgtc gcgctgcacc ggtacgatgg ccgcccccgc
     2281 ctcacctccc tgacgcagtg tcagatcgat cacctccgcc tggctctcca gggtctgggc
     2341 ctcgaagaag cactgcatcg gcagatcgag gtcgaaggca tcgcgcactc gggagcgcaa
     2401 ctgggcgacc ttgagggagt gcccgccgag gtcgaaaaag ttgtcgccga gcccgacgtg
     2461 accgatctcg agcagctcgc gccagagcgc ggccagcagc tcctgcaccg gcgtgacgag
     2521 cgtgtccgac tccgcgccgc tctcgccgcc ctgggccagg gcttggcgtc ctatctcgcc
     2581 gagagtgtgc cggtccacct tgccgctggt gctgagcggc cagacggtga cgggcacgaa
     2641 ggccgtggga accatgtggt cgggcagatg gcggcgggcg tgctcgcgca gctccaccga
     2701 cgctaccggc gcgcccagga tcaggaaggc cgccagccgg ccgtccaggg ccaggaccgt
     2761 cgcctcgcgc accgccgggt gcagggtcag caccgcctcg atctccccca actcgatacg
     2821 ctggccgcgg atcttgacct ggtggtcgat acgtcccaga aactcgatct cgccctcggg
     2881 cgaccagcgc accaggtcgc ccgtgcggta gaggcgctca cccggagagg cggcaagcgg
     2941 atcgggcacg aagcgttcgg cggtcaggtc ggggcggccc aggtagccgc gcgccaggcc
     3001 agcgccgccg atcagcagtt cgcccggcac gcccagaggc gccggacgtg gctcccctcc
     3061 gggctccagt acatggacgc ggacgttctc cagcggccgg ccgatggtgg ggacctgacc
     3121 cgcccactcg ggccggtcca tggcggacac cggcccggtg gtggccatca ccgtcgcctc
     3181 ggtggggccg taggcgttga tcaaccggaa gggaagcgac tccggcacga aacaatgcag
     3241 ccggtcgccg cccgtgagca ccgagcgcaa cgacatccga ggctctccag tgtcatccca
     3301 gcccagctcg aggagccctt cggcgagagc cgtgggcagg aaactcgtag tgatcccctg
     3361 ctgcgccatc cagttccgca gctcctccgg cgaggtgcgc accgcctccg gcgcgatgta
     3421 caggctggcg ccggccgcga gatagatcca gatctccagg attgtggcgt catagcccac
     3481 gccggccagg agcgtcgtgc ggtcggccgc ctggacgctc tgcaggcgag cctgcaatgc
     3541 caccaggttc agcagcgaac cgtgcgacag gtcgacggcc ttgggccggc cggtggatcc
     3601 ggaggtgtag atgacgtagg ccaggttgcc cggcgccagc gccggcgtcg gcagctcctc
     3661 gcccacaccg tggacgtcca actcgtgggg gaacaggcaa tggatgccgc gcttcgcggc
     3721 gagctcggcg agtctgccac gccgtgtgag caggaccggc atccccgagt cctcgaactg
     3781 gaagcccagc ctctcctcgg ggtgggacgg gtcgagagca acgtaggcgc cgccggcctg
     3841 gagcactccc aggcatgagg ccagcagctc cggcgagcgc tccaaacaga cgccgacccg
     3901 aacttcaggg ctcacgccca ggccgcgcag cgcgtaggcg atgcggcgcg cccagaggtc
     3961 gagcatgccg tagctcacct gccgcgcgcc gtcgaccatc gcaggcgcct ccggctggcg
     4021 ccgcgcctgc gccgccacca gctcgtgcac ccaggggcca ccggccgcct cgcgtgccgt
     4081 gtcgttccat tcggccagca actggtgccg ctcggcctcg gtgagcagcg gcagccgcga
     4141 cagcggcagg tccggcgcca tggcgatgcc ttccagcaga gtcgcgtaat gcgccagcaa
     4201 ccgcgccacg gtggaggggg tgaacaggtc caccgagtac tcggccgcga gcgtcagacc
     4261 ctcctcccgc tcctcgactg tgagcgacag gtcgaacttc gcggtctggc tctcgccgga
     4321 cgacagcggc gcgatgcgca gtgcaccctc gaccggggtg ggggtgttct ggagcgccat
     4381 cgtcacttgg accaccggat tgcgcgacag gtcgcgcggc ggccgcagct cctcgaccag
     4441 cgtgtcgaag gccagatcct ggttggcgaa ggcctccagc accgtctcgc gcacggtagc
     4501 cacgaaaccc agcgtttcca cgtcctccgc tgggcg
//
LOCUS       DASPJI010000482         6247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_375751, whole genome shotgun sequence.
ACCESSION   DASPJI010000482 DASPJI010000000
VERSION     DASPJI010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375751"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="VEL74_13100"
     CDS             <1..1614
                     /locus_tag="VEL74_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HYG63512.1"
                     /translation="EPKGEFRRGGEAITPDQAGEPLLELLRAGVLASDAHLSQGEEGA
                     WEVQGDPTEGALVVAAAKAGIDKAALDAERPRVGEIPFTSETKRMTTLHRFPDGTPVA
                     CEKGAPEVLLPSCSKVRTAEGTAPLDAAGRERILAAAGEMAAKALRVLAVAAKPGATL
                     EDAERDLVFLGLVGMIDPPRTEVKDAIRTCQEAGIKPVMITGDHPVTAEAIARELGVL
                     RGGRVLTGAELEDLDDATFDRMVGEVEVYARVSPAHKLRVVSVLQKKGDLVAMTGDGV
                     NDAPALKKADIGIAMGITGTDVTKEAADMTLTDDNFASIVAAVEEGRGVFGNIKKYLM
                     FLLSSNIGEIGLMAGASLLGLPLPLSAVQILYVNLATDGLPALALAVDPPEDDLMRRP
                     PRDPRTGIFTRPVVALMLVGGIWSTLVNLGLFVWALRSGRGLEEAMTMAFVSLVMIQF
                     FKSYNYRSDRQSVLHHPFANKWLNLAIVWELVLLALIVYLPVLQEPFGTFPLSAADLA
                     IIGGLSLTISPVLELAKWMERRGWFGRMD"
     gene            complement(1666..2979)
                     /locus_tag="VEL74_13105"
     CDS             complement(1666..2979)
                     /locus_tag="VEL74_13105"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HYG63513.1"
                     /translation="MRRTATFRAVTLLTLLAFTSSCMTTQLPPISSAGGDFEPLPDEQ
                     ALWEEARDEEKKLLEEADLYEDPLLEDYLEDVVSRLTPPGMAANEAVRYRVRVIEDPT
                     LNAFAYPHGSLYVHTGLLARMENEDQIATVLGHEMTHVENRHMVRYRRSAMNKQIGLS
                     VAAVAAAVILAGEQGDAMSRGQWGKAATIGVLGDVIVGLGLQLAFLASVNGYGRNLEH
                     EADQGGFAKLAATGYDLQEAPKVYQALLEDRGEKPEVEAFFFGSRPQLTLRIENARKY
                     LAAHPAAGDVSPAGDAEAFSRRIRPVVRDDARLNLEMGRLTLAEAQLEKVRQWMPDDP
                     EAHYLTGRLHLAKSETAEAEKDAAAQAELRAAARESLRESIRLDPERPGPHRELGLLL
                     HRGGELRDACRELRHYARLDPDADDAERVRDYIRDLERDGECPGE"
     gene            complement(3024..>6247)
                     /locus_tag="VEL74_13110"
     CDS             complement(3024..>6247)
                     /locus_tag="VEL74_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG63514.1"
                     /translation="NDGYIPVLGGKHPQALGQRGDECWAEIWDVVGPLYEQVIRTGEA
                     TFSEDLLLLMERSHYLEETYFTFSYSPVRDEQGDVAGNLITCTETTGRVVGERRLRTL
                     RDLGARAAEGKLAADACAIAAATLAANPWDVPFTLIYLLDGEGRRLRLTGSTGVPAGS
                     DAAPDIVELDATDTGRGWPLAEVLLGSGPVRVKQLAERFDRVPPGPWPDPPQTALALP
                     LAAPGQELPTGVLVAGVNPRRALDEGYLSFFALVAGQIATAITNARAYQEERRRAEAL
                     AELDRAKTAFFNNVSHEFRTPLTLLLGPLEDALEEGASPLSGQHRERIEAAHRNALRL
                     LKLVNTLLDFARIEVGRIEAMYEPTDLAAYTADLASVFRSAVERAGLRLAVDCPPLGE
                     PVYVDREMWEKIVLNLLSNAFKFTLDGEITVRLREVGDRVALSVTDTGVGIPAADLPQ
                     VFERFRRVRMPRARSMEGSGIGLALVSELVKLHGGQVAVESTPGQGSTFTVAIPRGSA
                     HLPAERIGGGRTLASTSLGAAPYVEEALRWLPPSAPASLEAPAGAPPPLSSSTARILL
                     ADDNADMRDYVRRLLGERWAVEAVDDGAAALAAALAAPPDLILADVMMPELDGFELLR
                     ELRADPRTRSLPVILLSARAGEESRIEGLEAGADDYLVKPFSARELIARVGAQLAAAR
                     VREEAGRERDELLAREREARREAEAASRAKDEFLAMLSHELRNPLGAISNAAHALEDA
                     AGADAYLQRLGRIVVRQSRHLSRLLDDLLDVARVTAGKIELRSQTVDLAEVAERCVRT
                     LQDSGRSQRHLLEHETSPAWVEGDPARLEQVLINLLENAVKYTPDGGTVRLTVGPEGD
                     DAVARIRDTGIGIEPGMLSRVFDLFVQGEQPLARTGGGLGLGLTLVRRLVEMHGGGVE
                     ARSDGAGRGSEFSVRLPLRAPAAAAQPGPALAAAGSARRVLIVEDNADSREALRLLLE
                     LDGHQVAAAEDGPQALELAASFVPDLVLLDIGLPGMDGYEVARSLGRHPERSRMRLVA
                     LSGYGQESDVGRSRDAGIDLHLVKPVDPGRLRELLAGFGAAPM"
BASE COUNT          829 a         2198 c         2346 g          874 t
ORIGIN      
        1 gagccgaagg gcgaattccg gcgcggcggc gaggcgatca ccccggatca ggccggcgag
       61 ccgctcctcg agctgctgcg cgccggcgtc ctcgcctcgg acgcccacct gtcgcagggg
      121 gaggaggggg cgtgggaagt ccagggggac cccaccgagg gggcgctggt ggtggccgcc
      181 gccaaggccg ggatcgacaa ggccgccctc gacgccgagc ggccgcgcgt gggcgagatc
      241 cccttcacct ccgagaccaa gcggatgacc acgctgcacc gcttccccga cgggaccccg
      301 gtggcctgcg agaagggggc gccggaggtc ctgctgccgt cctgctccaa ggtgcggacc
      361 gccgaaggca ccgcccccct cgacgccgcc ggacgggaga ggatcctcgc cgcggccgga
      421 gagatggcgg ccaaggcgtt gcgggtgctg gcggtcgccg ccaagcccgg cgccaccctg
      481 gaggacgccg agcgggacct cgtcttcctg ggcctggtgg ggatgatcga tccgccgcgg
      541 accgaggtga aggacgccat ccgcacctgc caggaggcgg ggatcaagcc ggtgatgatc
      601 accggcgacc atccggtgac cgccgaggcg atcgcccgcg agctcggcgt gctgcgcggc
      661 ggccgggtgc tcaccggcgc cgagctggag gacctggacg acgccacgtt cgaccgcatg
      721 gtgggggagg tggaggtcta cgcccgggtc tccccggccc acaagctgcg cgtcgtctcc
      781 gtccttcaga agaaggggga cttggtggcg atgaccggcg acggcgtcaa cgacgcgccc
      841 gccctcaaga aggccgacat cggcatcgcc atggggatca ccggcaccga cgtcaccaag
      901 gaggcggccg acatgaccct gaccgacgac aacttcgcct ccatcgtcgc ggcggtggag
      961 gaggggcgcg gggtcttcgg caacatcaag aagtacctga tgttcctgct ctcctcgaac
     1021 atcggcgaga tcggcctgat ggcgggggcg tcgctgctcg gcctgccgct ccccctgtcg
     1081 gcggtgcaga tcctctacgt caacctggcg accgacggcc tgccggccct cgccctggcc
     1141 gtggacccgc cggaggacga cctcatgcgc cggccgccgc gcgacccccg caccggcatc
     1201 ttcacccggc ccgtggtcgc cctgatgctg gtggggggga tctggtcgac gctggtgaac
     1261 ctggggctct tcgtctgggc cctgcgctcc ggccgcgggc tcgaggaggc gatgaccatg
     1321 gccttcgtct cgctggtgat gatccagttc ttcaagtcct acaactatcg ctccgaccgc
     1381 cagtcggtgc tccaccaccc gttcgccaac aagtggctga acctggcgat cgtctgggag
     1441 ctggtgctcc tggctctgat cgtctatctt cccgtgctgc aggagccgtt cggcaccttc
     1501 cccctgtcgg ccgccgacct ggcgatcatc ggcggcctgt cgctgaccat ctcgccggtg
     1561 ctggagctcg ccaagtggat ggagcggcgg ggctggttcg gccggatgga ctgaggagat
     1621 ctgcgggatg aaccgcctac ctggcgtcgc tgccggggga ttgacctact cccccgggca
     1681 ctccccgtcc cgctccaggt cccggatgta gtcgcggacg cgctcggcgt cgtcggcgtc
     1741 ggggtcgagc cgggcgtagt gccggagctc gcggcaggcg tcgcgcagct cgccgccccg
     1801 gtggaggagg aggcccagct cgcggtgcgg gcccgggcgc tccgggtcga ggcggatgga
     1861 ctcgcgcagc gactcgcgcg ccgcggcacg cagctcggcc tgcgccgcag cgtccttctc
     1921 ggcctcggcg gtctcagact tcgccaggtg caggcggccg gtgaggtagt gcgcctcggg
     1981 atcgtcgggc atccactgcc gcaccttctc gagctgggcc tcggccaggg tcagccgccc
     2041 catctccagg ttcaggcggg cgtcgtcgcg caccaccggc cggatgcgcc ggctgaaggc
     2101 ctcggcgtcg ccggccggag agacgtctcc ggccgcgggg tgggcggcga ggtatttcct
     2161 ggcgttctcg atgcgcaggg tgagctgcgg gcggctgccg aagaagaagg cttcgacctc
     2221 cggcttctct ccgcggtcct cgagcagcgc ctggtagacc ttcggcgcct cctgcaggtc
     2281 gtagccggtg gccgccagct tggcgaagcc cccctggtcg gcctcgtgct ccaggttacg
     2341 gccgtagccg ttgaccgagg cgaggaaggc gagctggagc cccaggccga cgatgacgtc
     2401 gcccagcacg ccgatggtgg cggccttccc ccactgcccc ctgctcatgg cgtccccctg
     2461 ctcgccggcg aggatgaccg ccgcggccac ggcggcgacg ctgaggccga tctgcttgtt
     2521 catcgcgctc cgccggtagc gcaccatgtg ccggttctcc acgtgcgtca tctcgtgacc
     2581 gaggacggtg gcgatctggt cctcgttctc catccgcgcc agcaggccgg tgtggacgta
     2641 gagcgagccg tgggggtagg cgaaggcgtt gagcgtcggg tcttcgatca cccgcacgcg
     2701 gtagcggacc gcctcgttgg ccgccatgcc cggcggcgtc aggcgggaga ccacatcctc
     2761 cagatagtcc tcgaggagcg ggtcctcgta gaggtccgcc tcctcgagga gcttcttctc
     2821 ctcgtcccgc gcctcctccc agagcgcctg ctcgtcgggc agcggctcga agtcccctcc
     2881 cgcgctggag atcggcggga gctgcgtggt catgcacgag ctggtgaagg cgaggagcgt
     2941 caacagggtg acggcacgga acgtcgcggt gcggcgcata gaggcctcca ggaaggagcg
     3001 gggtactgcg gaaattgtag cagctacatg ggcgccgcgc cgaaaccggc cagcagctcc
     3061 cgcaggcggc cgggatcgac cggcttgacc aggtggaggt cgatgccggc gtcgcgggag
     3121 cggccgacgt cgctctcctg gccgtagccg ctcagggcga cgaggcgcat gcgggagcgc
     3181 tccgggtggc gccccaggga gcgggccacc tcgtagccgt ccatgcccgg cagcccgatg
     3241 tcgaggagga ccaggtccgg gacgaagctc gcggccagct ccagggcctg ggggccgtcc
     3301 tcggcggcgg cgacctggtg gccgtccagc tccaggagca ggcggagggc ctcgcgcgag
     3361 tccgcgttgt cctcgacgat cagcacgcgg cgggccgagc cggcggccgc cagcgccggc
     3421 ccgggctgcg ccgccgccgc cggcgcccgc agggggaggc gaaccgagaa ctcgctgccg
     3481 cgcccggcgc cgtcgctccg cgcctccacc cctccgccat gcatctccac cagccggcgg
     3541 accagggtga ggcccagccc caggccgccg ccggtgcggg cgagcggctg ctccccctgc
     3601 acgaagaggt cgaagacgcg cgacagcatc ccgggctcga tgccgatgcc ggtatcccgg
     3661 atgcgggcga cggcatcgtc cccctcgggc cccacggtca ggcggacggt gcccccgtcg
     3721 ggggtgtact tgacggcgtt ctccaggaga ttgatgagca cttgctcgag ccgcgcgggg
     3781 tctccctcga cccaggcggg ggaggtctcg tgctccagga ggtggcgctg ggacctgccg
     3841 ctgtcctgga gcgtgcgcac gcaacgctcg gcgacctcgg cgaggtcgac ggtctggctc
     3901 cgcagctcga tcttgcccgc ggtgacccgc gccacgtcga gcaggtcgtc gagcaggcgg
     3961 gagaggtggc ggctctggcg gacgacgatg cggccgagcc gctgcaggta ggcgtccgcg
     4021 cccgccgcgt cctccagggc atgggcggcg ttgctgatcg cccccagggg gttgcgcagc
     4081 tcgtggctga gcatggcgag gaactcgtcc ttggcgcggc tggccgcctc ggcctcgcgc
     4141 cgggcctccc gctcgcgggc gagcagctcg tcgcgctccc ggccggcctc ctcgcgcacc
     4201 cgcgccgccg cgagctgggc gccgacccgg gcgatcagct cgcgggcgga gaaaggcttg
     4261 accagatagt cgtcggcgcc ggcctccagc ccctcgatcc gcgactcctc gccggcgcgg
     4321 gcggagagca ggatgaccgg cagggagcgg gtgcgcggat cggcgcgcag ctcgcgcaac
     4381 agctcgaagc cgtcgagctc cggcatcatc acgtcagcga ggatcaggtc ggggggagcg
     4441 gccagcgccg ccgccagggc cgcggcgccg tcgtccaccg cctcgacggc ccagcgctct
     4501 ccgagcagcc ggcgcacata gtcgcgcatg tcggcgttgt cgtcggcgag gaggatgcgg
     4561 gccgtggacg aggagagcgg gggaggggcg ccggccggcg cctccaggct cgccggcgcc
     4621 gaggggggca gccagcgcag cgcctcctcg acgtacggtg cggcgccgag gctggtggag
     4681 gcgagggtcc ggccgccgcc gatccgctcc gccggcaggt gggcgctccc cctggggatg
     4741 gccacggtga acgtggagcc ctgccccggc gtgctctcca ccgcgacttg gccgccgtgc
     4801 agcttcacca gctcggagac cagcgccagg ccgatgcccg acccttccat ggagcgcgcc
     4861 cggggcatgc ggacacgccg gaagcgctcg aagacctggg ggaggtcggc cgccgggatg
     4921 cccaccccgg tatcggtcac cgacagcgcc acccggtcgc ccacctcccg caggcgcacg
     4981 gtgatctcgc cgtcgagggt gaacttgaag gcgttggaca gcaggttgag gacgatcttc
     5041 tcccacatct cgcggtcgac gtagaccggt tcgccgagcg gcgggcagtc gacggccagg
     5101 cggagccccg cgcgttcgac ggcggaccgg aagacgctgg cgaggtcggc ggtgtaggcg
     5161 gcgaggtcgg tgggctcgta catcgcctcg atccggccga cctcgatgcg cgcgaaatcg
     5221 agcagggtgt tcaccagctt gagcagccgc agcgcgttgc ggtgcgcggc ctcgatgcgc
     5281 tcccggtgct gcccggacag cggcgaggcg ccctcctcga gcgcgtcctc gagcgggccg
     5341 aggaggaggg tgagcggggt ccggaattcg tggctgacgt tgttgaagaa ggcggtcttg
     5401 gcgcggtcga gctcggccag cgcctcggcc cgccggcgct cctcctggta ggcgcgggcg
     5461 ttggtgatgg cggtggcgat ctggccggcg acgagcgcga agaagctgag atagccctcg
     5521 tcgagcgcgc ggcgcgggtt gacgccggcc accaggacgc cggtgggcag ctcctggccc
     5581 ggcgccgcca gcggcagggc gagggcggtc tgcggcgggt ccggccaggg tccgggaggg
     5641 acgcggtcga agcgttcggc gagctgcttg acccgtaccg gcccgctgcc gaggaggacc
     5701 tcggccagag gccagccacg tcccgtatcg gtggcatcca gctcgacgat gtccggggcc
     5761 gcgtcgctgc cggccggaac cccggtgctc cccgtgagcc ggaggcggcg gccctcgccg
     5821 tcgagcagat agatcaaggt gaacggcacg tcccacgggt tggcggccag ggtcgcggcg
     5881 gcgatggcgc aggcatccgc ggccagcttg ccctcggccg cgcgggcgcc gagatcgcgc
     5941 agggtgcgca accgccgctc gccgacgacc cgcccggtcg tctcggtgca ggtgatgagg
     6001 ttgccggcga cgtccccctg ctcgtcgcgg accgggctat aggagaaggt gaagtaggtc
     6061 tcctccagat agtggctgcg ctccatgagc agcaggaggt cctcggagaa agtggcctcg
     6121 ccggtccgga tcacctgctc gtacagtggg ccgacgacgt cccagatctc ggcccagcac
     6181 tcgtcaccgc gctgtcccag ggcctgaggg tgcttgccac ccagaaccgg tatatagccg
     6241 tcgttgt
//
LOCUS       DASPJI010000483         6769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_493734, whole genome shotgun sequence.
ACCESSION   DASPJI010000483 DASPJI010000000
VERSION     DASPJI010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493734"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1647)
                     /gene="acnA"
                     /locus_tag="VEL74_13115"
     CDS             complement(<1..1647)
                     /gene="acnA"
                     /locus_tag="VEL74_13115"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HYG63515.1"
                     /translation="MKDSFGSRRTLTAGGQTVSIAALPALEQRGWNVSRLPYSLRILL
                     ENLLRREDGEVVSAADIEALATWDPKAEPSREIAFMPGRVLLQDFTGVPAVVDLAAMR
                     DAMAAMGGDPKRINPLQPVELVIDHSVQVDEYGSAAAFLINAEKEFERNRERYAFLRW
                     GQTAFDNFRVVPPDTGIVHQVNLEYLARVVMTGEQDGETWGYPDTLVGTDSHTTMVNG
                     LGVLGWGVGGIEAEAAMLGQPVSMLIPQVVGFRLAGELPEGTTATDLVLTVTEMLRKK
                     GVVGRFVEFFGPGLSTLPLADRATIANMAPEYGATCGFFPVDHATLQYLTFTGRSPEQ
                     VELVEAYMREQGLFLTQESPDPVYTDTLELDLSTVEPSVAGPRRPQDRVRLKDVKASF
                     LKELDGMVGNGKTGAVRKAGPGLVTVGGTAAGVAVAEPELAALPVTGHADAAEALRHG
                     SVVIAAITSCTNTSNPSVMIGAGLLAKKAVERGLDVKPWVKTSLAPGSKVVTEYLKEA
                     GLTPHLEALKFHLVGYGCTTCIGNSGPLPDPIAEAITDSDL"
     gene            complement(1779..1904)
                     /locus_tag="VEL74_13120"
     CDS             complement(1779..1904)
                     /locus_tag="VEL74_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63516.1"
                     /translation="MADEDIDTTDIPPLDKKFFANAELRSPRPKPDMAHQKPTRS"
     gene            2083..2670
                     /locus_tag="VEL74_13125"
     CDS             2083..2670
                     /locus_tag="VEL74_13125"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HYG63517.1"
                     /translation="MGQTDFELSPPILLVAMPQVLDPFFHKSVVLLLHHEDEGSFGFI
                     INRATGIKVNEILQGMEVGWQGGRDLVAYFGGPVQPQLGTVLFSPEEVEGDAATEVIP
                     GLALTQHIGDLAQLAESPPGQFRLLLGYAGWGAGQLMEEILRNDWLTAPPQAGLIFAP
                     DPEQVWGEALRSVGVDPSLLPSWTTPVPGDGEAAN"
     gene            2628..3254
                     /locus_tag="VEL74_13130"
     CDS             2628..3254
                     /locus_tag="VEL74_13130"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HYG63518.1"
                     /translation="MDHAGAGGRGGGQLRRRRRGRGGPPRAASRAATAPAPLSRLQTE
                     HHRSAGGLVVDGSRILLISTQTGRRWQLPKGHVEAGETSEEAAVREVREETGVTGRIL
                     ANLPEVEYWYVERGPRRIHKRVEYFLLDYVEGDCANYDPREVSGAEWFSWETGLAKLT
                     FENERRVVEQARRLAHGGEAPVSPAESAAASAVEVRVEIRSASEEAPP"
     gene            3251..3598
                     /locus_tag="VEL74_13135"
     CDS             3251..3598
                     /locus_tag="VEL74_13135"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HYG63519.1"
                     /translation="MIRALLHIAANAGGLVIADRVVEGINYDGDLLNLLLAGLVIGLI
                     NLLVKPIVSFFSFPLIVVTLGLFYLVINGLMLYLAAYLLRGLSIDGCLPAILGGLIIA
                     VLNWVVGALVPDR"
     gene            3666..4325
                     /gene="nth"
                     /locus_tag="VEL74_13140"
     CDS             3666..4325
                     /gene="nth"
                     /locus_tag="VEL74_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018399876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HYG63520.1"
                     /translation="MAQARRESGRARKARVAEILERLRQAYPGARVELDFQSPYQLLV
                     ATILAAQCTDERVNQVTPALFQRYPDPRHLAAAELPDLEEMVKTTGFFRNKARAIKAM
                     ATALVAEHDGEVPPRMDALVHLPGVGRKTANVILGNAFGLDEGVVVDTHVTRLSRRLG
                     LTDEDDPVKIEADLMAVVPREAWTRWSNQLILHGRRLCKARKPECPRCPVAELCPSAE
                     V"
     gene            4434..5186
                     /locus_tag="VEL74_13145"
     CDS             4434..5186
                     /locus_tag="VEL74_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYG63521.1"
                     /translation="MTRQRIAVVTGASSGIGAATARILAGEGFRVVIGARRLDRLREV
                     ADPIGATALPLDVTDPASVAAFVAAVPEADVLVNNAGGALGLDRLEDSSDETWQRMWE
                     TNVLGTVRLCRAFIPALVASGDGHVVTIGSTAGHEVYPGGSGYTSAKHAQRAVTRTLR
                     QELLGRPVRVTEVDPGLVETEFSVVRFHGDRERAAAVYQGLTPLTAEDVADCVRWAVT
                     RPSHVNIDEIVLRPRAQATSTLVHREPQGGRA"
     gene            5183..5557
                     /locus_tag="VEL74_13150"
     CDS             5183..5557
                     /locus_tag="VEL74_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYG63522.1"
                     /translation="MSPRTVAGMAGSALALTAAVTLSACGAVFPQRSEGEKLYRKHCA
                     ECHGLDAAGNTPGYMGNAYADLRDDVWKTGGDSDSMQSVVRNGIFAKMPAYDQLSAKE
                     VRQVVDYVRELRGEKMPEARTR"
     gene            5608..5994
                     /locus_tag="VEL74_13155"
     CDS             5608..5994
                     /locus_tag="VEL74_13155"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYG63523.1"
                     /translation="MGGRVGGAHTKEPGRAGSARAWGALVACLLLMLGGCARDRSAPG
                     LYRSYCRRCHGAEGEGKPRALKLYPKSNLLISEMVLQGDREAVRDRIANGEGPMPAFK
                     RRLTPEELESLVDFTIRLASEQKRKE"
     gene            5996..6244
                     /locus_tag="VEL74_13160"
     CDS             5996..6244
                     /locus_tag="VEL74_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63524.1"
                     /translation="MDPQRMKEAYQKLQSLDERLTHKVRPRSGGSMTRPSAEALEQGM
                     RDLATYTVELKEVVQELFLAIAGRPQQGPPQGGGGAGG"
     gene            6335..>6769
                     /locus_tag="VEL74_13165"
     CDS             6335..>6769
                     /locus_tag="VEL74_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63525.1"
                     /translation="MSYLKLTAALALLMLAGTTAAVASPATDTAFFQSLAEGSLPAQQ
                     EEPLAGVGTPEPTFKSCSISRPCGDGNYASCTGNSSCINSQKGVKCDGTEYACPNYCS
                     MSWQCENCPTYVHICWSLKGDCGVSGAGCDGRPQRCICPEQPI"
BASE COUNT         1009 a         2320 c         2435 g         1005 t
ORIGIN      
        1 gagatcgctg tcggtgattg cctcggcaat ggggtccggc agggggccgc tgttgccgat
       61 gcaggtggtg cagccgtagc cgacgagatg gaacttgagc gcctcgaggt gcggggtgag
      121 cccggcctcc ttgaggtact cggtgaccac cttcgagccg ggggcgaggc tggtcttgac
      181 ccacggcttg acgtcgaggc cacgctcgac cgccttcttg gcgagcaggc cggcgccgat
      241 catgaccgac gggttcgaag tgttggtgca gctcgtgatc gcggcgatga ccaccgagcc
      301 gtgccggagc gcctcggcgg catcggcgtg gccggtgacc ggcagcgcgg cgagctcggg
      361 ctcggcgacc gcgacgccgg cggccgtgcc gcccaccgtc accagccccg ggcccgcctt
      421 gcgcacggcg ccggtcttgc cgttgccgac catcccgtcc agctccttga ggaacgaggc
      481 cttgacgtcc ttcagccgca cccggtcctg cggccggcgc ggcccggcga cgctgggctc
      541 gacggtcgag aggtcgagct ccagggtgtc ggtgtagacc gggtccgggc tctcctgggt
      601 gaggaacagc ccctgctccc gcatgtaggc ctcgaccagc tccacctgct cgggcgagcg
      661 gccggtgaag gtcaggtact ggagcgtcgc gtggtccacc gggaagaagc cgcaggtggc
      721 gccgtactcg ggggccatgt tggcgatcgt ggcgcggtcg gcgagcggca gggtggagag
      781 gcccgggccg aagaactcga cgaagcggcc gacgaccccc ttcttgcgca gcatctcggt
      841 gacggtgagc acgaggtcgg tcgccgtggt cccctcgggc agctcgccgg cgagccggaa
      901 gccgaccacc tgggggatca gcatggacac cggctggccg agcatcgccg cctcggcctc
      961 gatgccgccg acgccccagc cgaggacgcc gaggccgttg accatggtgg tgtgggagtc
     1021 ggtcccgacg agggtgtcgg ggtagcccca ggtctcgccg tcctgctcgc cggtcatgac
     1081 gacgcgggcg aggtactcga ggttgacctg gtggacgatg ccggtgtccg gcggcacgac
     1141 ccggaaattg tcgaacgcgg tctggcccca gcgcaggaag gcgtagcgct cgcggttcct
     1201 ctcgaactcc ttctcggcgt tgatgaggaa ggcggcggcg ctgccgtact cgtccacctg
     1261 cacggagtgg tcgatgacca gctcgacggg ctggagcggg ttgatccgct tgggatcgcc
     1321 ccccatggcg gccatggcgt cgcgcatcgc ggcgaggtcg accacggcgg ggacgccggt
     1381 gaagtcctgc agcaggacgc ggccgggcat gaaggcgatc tcgcgcgacg gctcggcctt
     1441 ggggtcccag gtggcgaggg cctcgatgtc ggcggcgctg accacctcgc cgtcctcgcg
     1501 gcggagcagg ttctccagca ggatgcgcag cgagtagggc agccgggaga cgttccatcc
     1561 gcgctgctcc agggcgggga gggccgcgat ggagacggtc tggcctccgg cggtgagcgt
     1621 cctccgactt ccgaagctat ctttcatcga ctctctccaa tcgggcaatc agcaggcatg
     1681 ggggaccggc gagacgccgc ctccgggcag gagggggacg cccggacttg gaaagatatc
     1741 aggtggaagg caggaggggc cagcgggggg ccgggccgtc agctccgggt aggcttctgg
     1801 tgcgccatgt ccggcttcgg ccgcggcgaa cgcagctccg cgtttgcaaa gaactttttg
     1861 tcgagcggag ggatgtccgt ggtgtcgatg tcctcatcgg ccatcgagtc gatccgctgc
     1921 cagtcagtcc gtgacgttct ctcaatacct ccgtcgctca ttcctgggcc agtttatgga
     1981 aaaggtgtat gcacttcaac cgcgatgaag gttcgcgagc tcccagcaat ctgcctctcc
     2041 atccgccggt cctcccacct cagtagtagt aagatgatct tcatggggca gacggacttc
     2101 gagctctcgc cgccgatcct gttggtggcc atgccgcagg tgctggaccc gttcttccac
     2161 aagagcgtcg tcctgctcct ccaccacgag gacgagggga gcttcggctt catcatcaac
     2221 cgcgccacgg ggatcaaggt caacgagatc ctccagggga tggaggtggg gtggcagggg
     2281 gggcgggacc tggtggccta cttcggcggc ccggtccagc cgcagctcgg cacggtcctg
     2341 ttcagccccg aggaggtgga gggggacgcg gcgaccgagg tcatccccgg gctcgcgctc
     2401 acccagcaca tcggcgacct ggcgcagctc gccgagtcgc ctcccggcca gttccggctg
     2461 ctcctcggct acgccggctg gggcgccggc cagctcatgg aggagatcct ccgcaacgac
     2521 tggctgaccg cgccgcccca ggccggcctg atcttcgcgc cggacccgga gcaggtctgg
     2581 ggcgaggctc tgcgctcggt cggcgtcgac ccgtcgctgc tcccctcgtg gaccacgccg
     2641 gtgccggggg acggggaggc ggccaactga gacgccggcg gcgcgggcgg ggcgggcctc
     2701 cacgcgctgc ttcccgggcg gcgaccgcgc cggcgcctct gtcccgcctg cagaccgagc
     2761 accaccgctc ggccggcggg ctggtggtcg acggctcccg catcctcctc atctccaccc
     2821 agaccggccg ccgctggcag ctccccaagg ggcacgtcga ggcgggggag acctccgagg
     2881 aagccgccgt ccgcgaggtg cgggaggaga ccggcgtcac cggccgcatc ctcgccaacc
     2941 tgccggaggt cgagtactgg tacgtggagc gcggaccccg ccgcatccac aagcgggtcg
     3001 agtacttcct cctcgactat gtcgaggggg actgcgccaa ctacgacccg cgcgaggtct
     3061 ccggcgccga gtggttctcc tgggagaccg gcctcgccaa gctcaccttc gagaacgagc
     3121 ggcgggtggt ggagcaggcc cggcgattgg cgcacggagg ggaggcgccc gtatctccgg
     3181 cggaatcggc ggcggcttcg gcggtcgagg tccgggtcga gataagatcc gcctccgagg
     3241 aggcacctcc atgattcgag cgctgcttca catcgcggcc aacgccggcg gcctggtcat
     3301 cgccgaccgt gtggtcgaag gcatcaacta cgacggcgac ctgctgaacc tgctgctggc
     3361 gggcttggtc atcggcctga tcaacctgct ggtcaagccg atcgtcagct tcttctcgtt
     3421 ccccctcatc gttgttaccc tggggctctt ctacctggtg atcaacggcc tgatgctcta
     3481 cctcgccgcc tacctcctgc ggggcttgag catcgacggc tgcctgccgg cgatcctcgg
     3541 cggcctgatc atcgccgtgc tcaactgggt ggtgggggcg ctggtccccg accgctgaag
     3601 gggtactgat ggcgacgaag aaggcgatac ccccggcggc ggcccccgcg agggccggga
     3661 tcaccatggc ccaggcgcgg cgcgagtcgg gccgcgcccg caaggcgcgg gtggcggaga
     3721 tcctggagcg gctgcggcag gcctatccgg gtgcccgggt ggagctcgac ttccagagcc
     3781 cctaccagct cctggtggcg accatcctcg ccgcccagtg cacggacgag cgggtcaacc
     3841 aggtgacgcc ggcgctcttc cagcgctacc cggacccccg gcacctggcc gccgccgagc
     3901 tgccggacct cgaggagatg gtgaagacca ccggcttctt ccgcaacaag gcgcgggcga
     3961 tcaaggcgat ggcgaccgcc ctggtggcgg agcacgacgg cgaggtgccg ccgcgcatgg
     4021 acgccctcgt ccacctgccg ggggtcggcc gcaagaccgc caacgtcatc ctcggcaacg
     4081 ccttcggcct cgacgagggg gtggtggtcg acacccacgt cacccgcctg tcgcgccgcc
     4141 tcggcctcac cgacgaggac gacccggtga aaatcgaggc cgacctgatg gccgtggtcc
     4201 cccgcgaggc ctggacccgc tggtcgaacc agctcatcct ccacggccgc cggctgtgca
     4261 aggcccgcaa gcccgagtgc ccccgctgcc cggtggccga gctgtgccct tcggccgagg
     4321 tgtaggggtc ggtgtagggg agcgcggcag cccccttgct gctaccttat atatagatag
     4381 aaatggacag acagaggccg cggggtgaga gccccgcctg gggaggagcc tgcatgacac
     4441 gtcaacgtat cgccgtggtg accggcgcca gctcggggat cggggcggcc acggcgcgga
     4501 tcctggccgg ggagggcttc cgggtggtga tcggcgcgcg gcgcctcgac cggctgcgcg
     4561 aggtggccga tcccatcggc gccacggctc tcccgctgga cgtcaccgat ccggcgagcg
     4621 tcgccgcctt cgtggccgcg gtgccggagg cggacgtcct ggtcaacaac gccggcggag
     4681 cgctgggcct cgaccggctg gaggactcca gcgacgagac ctggcagcgg atgtgggaga
     4741 ccaacgtcct cggcaccgtt cgcctgtgcc gcgccttcat ccccgccctc gtggcgtcgg
     4801 gggacgggca cgtggtcacc atcggctcca ccgccgggca cgaggtctat cccggcggct
     4861 cggggtacac cagcgccaag cacgcccagc gggcggtcac ccgcaccctg cgccaggagc
     4921 tgctcggccg gccggtgcgg gtcaccgagg tggaccccgg cctggtggag accgagttct
     4981 cggtggtgcg cttccatggc gaccgggaac gggcggcggc cgtctaccag gggctgacgc
     5041 cgctcaccgc cgaagacgtc gccgactgcg tccgctgggc ggtcacccgt ccgtcccatg
     5101 tcaatatcga cgagatcgtg ctgcgtcccc gggcccaggc cacctcgacc ctggtccacc
     5161 gggagccgca gggaggccgc gcatgagtcc ccgtactgtc gccggcatgg ccggctccgc
     5221 gctggcgctg accgccgccg tgaccctgtc cgcctgcggg gcggtcttcc cccagcgttc
     5281 ggagggtgag aagctctacc gcaagcattg cgccgagtgc cacggcctcg acgccgccgg
     5341 caacaccccg ggctacatgg gcaacgccta cgccgacctg cgggacgacg tctggaaaac
     5401 gggtggcgac tcggactcca tgcagtccgt ggtgcgcaac ggcatcttcg ccaagatgcc
     5461 cgcgtacgac cagttgagcg ccaaggaggt gcggcaggtg gtggactacg tccgagagct
     5521 gcgcggcgag aagatgcccg aggcccggac gcgttgaagg gggtgaggga gcgaggggcg
     5581 tccggcccgg cacaggcgac tggcagcgtg gggggcaggg tggggggagc gcacacgaag
     5641 gagccgggca gggccggatc ggcccgcgcg tggggggcgc tcgtcgcctg tctgctcctg
     5701 atgctgggcg gctgcgcgcg ggaccggagc gcgccggggc tgtaccgctc ctactgccgg
     5761 cgctgccacg gcgccgaggg ggaggggaag ccccgggcct tgaagctcta ccccaaatcg
     5821 aatctgctga tctcggagat ggtcctccag ggggaccgcg aggccgtccg ggaccgcatc
     5881 gccaacggtg aaggcccgat gccggccttc aagcgccggc tcaccccgga ggagctggag
     5941 agcctggtcg acttcacgat ccggctcgcg tccgagcaga aaaggaagga gtgacatgga
     6001 tccccagcgg atgaaagagg cctatcagaa gctgcagagc ctcgacgaac ggctcaccca
     6061 caaggtccgc ccgcgcagcg gcggctcgat gacccgcccc tccgccgagg cgctcgagca
     6121 gggcatgcgc gacctcgcca cctacaccgt cgagctcaag gaggtcgtcc aggagctctt
     6181 cctcgccatc gccggtcgcc cccagcaggg cccgccgcag gggggcgggg gcgcgggagg
     6241 gtaggcgtcg tatttccagc cggcggtcca gccgaagaca gaagagagta aaatgcagtc
     6301 attactgtcc ttcggcaaga agacaaggag acttatgagc tacctgaagc tgaccgccgc
     6361 cctcgctctc ttgatgctgg ccgggaccac cgccgccgtc gcctctccgg ccacggacac
     6421 cgctttcttc cagtcgctcg ccgagggatc tctgccggcg cagcaggagg agccgctggc
     6481 cggggtcggg acccccgagc cgaccttcaa gtcctgcagc atctcccggc cctgtggcga
     6541 cggcaactac gcctcctgca cgggcaactc ctcctgcatc aactcgcaga agggcgtcaa
     6601 gtgcgacggc accgagtacg cctgcccgaa ctactgcagc atgtcctggc agtgtgagaa
     6661 ctgccccacc tatgtccaca tctgctggag cctgaaaggt gattgcggcg tcagcggcgc
     6721 aggctgcgac ggccgtccgc agcgctgcat ctgtccggag cagccgatc
//
LOCUS       DASPJI010000484         9919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_89258, whole genome shotgun sequence.
ACCESSION   DASPJI010000484 DASPJI010000000
VERSION     DASPJI010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9919)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9919
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89258"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VEL74_13170"
     CDS             complement(<1..204)
                     /locus_tag="VEL74_13170"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HYG63526.1"
                     /translation="MRPLRTPLTDLLGIDVPVLQSGMRQVAGPELAAEVARAGGLGIL
                     AGVRIGPDELRAQIHRLRERTDRP"
     gene            531..944
                     /locus_tag="VEL74_13175"
     CDS             531..944
                     /locus_tag="VEL74_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG63527.1"
                     /translation="MTSEIVLDASAAIHLIMAAEHASDLVERLERAASVAAPDLFFSE
                     VGNALWKYVQFRTLTVEKAVTHLQEAMDLVDGIVPTQELIQEAVIAAARYGHPVYDML
                     YAVLARRHGATVITTDRSFASLLRRMEIDTYCPLM"
     gene            1062..1541
                     /locus_tag="VEL74_13180"
     CDS             1062..1541
                     /locus_tag="VEL74_13180"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYG63528.1"
                     /translation="MSEELSAIAREIQQRRPFRTKGQEVVVSLLRTSDVLRRFVTRVL
                     EPYGITPQQYNVLRILRGAGEDGCPTLSIGERMIEEAPGITRLIDRLEIKGLVRRQRC
                     VEDRRQVLCWLTSEGRALVDRIDEPMDTADDEAVGMLSSEEQTTLCELLDKIRAGHR"
     gene            1604..2212
                     /locus_tag="VEL74_13185"
     CDS             1604..2212
                     /locus_tag="VEL74_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HYG63529.1"
                     /translation="MKRVLLTAVALSSILSLPVQAAEVHKIDPTHSEVSFQIRHMVTQ
                     VRGNFNEYEGVIHLDPANLEGSKVDLKIKAASIDTNLADRDKHLRAPDFFDVEKYPEI
                     TFRSTKIKKTGKDTYDVTGNFTMRGVTKQITLPVTYLGSAKDPWGGQRAGFETSTTLD
                     RKDYGINWNTALDNGGVILGDDVKVSINLETIQQKDANAKGK"
     gene            2490..3902
                     /locus_tag="VEL74_13190"
     CDS             2490..3902
                     /locus_tag="VEL74_13190"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYG63530.1"
                     /translation="MSHARRLVLFFSVSLLLALLLAIPGLAATDFSSLEKAVQSDLAA
                     TGTPGAAVIVVSGDRVVFAKGFGTANVEIGETGAPVTLDTLFQVGSVTKLLTATAVVA
                     YAQEGKLRLDAPLGTYVKGLAPRVAEVTVAQLLSQTSGMRDEPAEYGPHDESALLAAA
                     RAWKDSDRPLPPGTAFSYSNPGLALAGLAFQEAAGKPFADLMDERLFTPLGMSHSTFR
                     PTEAMTRPLSMGHDAKGEGKPTVVRPMADDARFWPAGSTLYTSANDLARFVRAFLNGG
                     RLDGRPVLSPAVLSQVATPHADIPALFEDGHYGYGLMLFREKGLRVAEHAGTMTGFSG
                     LIRMVPEHHFGLIVLANRSGVRLRATTDEAMRLLLPVKADAPPAERPSLPVSAEERAR
                     LTGFYSARWPMEVFEKEGRLFLRRFDQELEMTKVGDRRFAIRPPGADRPQELVFGPDG
                     PGGHPLYLHMALWAFARAAG"
     gene            3899..4378
                     /gene="ybaK"
                     /locus_tag="VEL74_13195"
     CDS             3899..4378
                     /gene="ybaK"
                     /locus_tag="VEL74_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HYG63531.1"
                     /translation="MKTNAARILDALGIGYELREYEVDPEDLAAETVARKVGLPAEQV
                     WKTLVARGDRTGVLLAVVPGDAELDPKALARATGDRKVDTVPLREVQPLTGYIRGGVT
                     ALGGKKDYPVWVDETVELYDLISVSAGVRGTQILLSPADYLRATRGKVAPISRPKEL"
     gene            4438..5559
                     /locus_tag="VEL74_13200"
     CDS             4438..5559
                     /locus_tag="VEL74_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63532.1"
                     /translation="MSQAATLLPLPPVPRPAVVSRPRTVRLETSVPRLLHVTNGDCAA
                     DLLRLSGIPGTVTLTADVLHDGPVPPPTLAPERWRKVRARFLAESGYEGYDECLARLT
                     EWDRALEAFRSYEEVVLWFEHDLFDQLHLIRLLDWFGRREVGWTRLSVVCIGEFPGIQ
                     RFRGLGQLEPMDFTSLLELRKPVTDEQIRLARNAWRVFCSAEPIGLELLLRRDTSALP
                     FLAGALLRHLEEFPSTRNGLSRTEHQGLLAVAEVAGDGGAATFAEVFQAAQDKEERAF
                     LGDLPFLQRLREMASRPRPLLRIEPGSDDSMAALQVTITPAGLEVLDGREDWVHIHGI
                     DRWLGGVHLVGFESQWRWDPDRQRLAPTWAPAPPASAWP"
     gene            complement(5562..7490)
                     /locus_tag="VEL74_13205"
     CDS             complement(5562..7490)
                     /locus_tag="VEL74_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HYG63533.1"
                     /translation="MSPPAPEPKTRLRFEDFELDPESGDLRRGGETVKLAPQPFRILA
                     LLAQRAGEPVSRDEIRRQVWGEETYVDFERGLNFCISQIRAALGDDAQSPRFVQTLPR
                     RGYRFIAPVERVPKAAIPQLVEEAVEAPPAPLPLPQVPRLGSQPSRLLAWAVAAVLGL
                     LLVIAFVAWRHRDGGTEPRLMIAVLPFEDLSAGPPEEYWSDGLTGELIAQLGRVRPER
                     LGVIARTSVMAYKGARRDVEELGDELGVDYLLEGSVRRAGDRVRITAQLVHADDRTQL
                     WSETYDRDRRDVLDVQAEVGAEVVRALQLELLPESARQPRTPPDPRAWDDYLRGRYLA
                     HRGDHESLRRSLDPYERAVRREPAFAEAHAAMADALHRLVMSGGLSPRQGYPRAEAAA
                     RRAVALDSGLAEAHAVLGAILFWYRWDWPAAERELRRALELNPSLPEARHDYGFFLIA
                     RGRADEGLDQVDRARDLDPLSARANVDVGWAAIGARRYDEAVARSQRTLELVPGFGEA
                     QFCMEQAYTLQGLHREAWRLASTRWARADATPGERAAIERLGLETREPEEALRAFYLW
                     NLQRLEAAAAQGSQVNSASVASLWAFVGNEDRAFDWLEKAVEERSPTLTLLAVHPSWD
                     GLRADPRFAELLKKVGPG"
     gene            complement(7727..8977)
                     /locus_tag="VEL74_13210"
     CDS             complement(7727..8977)
                     /locus_tag="VEL74_13210"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HYG63534.1"
                     /translation="MDMTRSTFLKTLSLAAAGCALPGGLSLAADIQTQTPPAELELLE
                     AVRSGDTQRVEALLAKEPGLIDRALPGGGTALHAAVRAGQGAMVSFLVGKGADSNALS
                     TDPPGFTPLRWAAEHADLATAELMVDAMVVNGADPNARQADGVSPLHAAAAAGNIEVI
                     QALIYDGADPEARTPDGKTALQLAADREAADLLRRHRDLPRDHSTSRFAYTATGAPFV
                     RQDTSSPLPIALIHEYVAVSHGSIARVRELLASHPGLLLTRARWDEMAVEAAAHVGNR
                     EIVEFQLERGAPLSLCTAAMMGMLDRVGALLREDPKRVRETGAHNIPLTWFPVVGGGH
                     LEVMKALLQAGTDVNSHKRGQAALHLAARTGQLEMASLLLDRGADVNARAKTAQGVLT
                     PLAMATQASKEEMVEMLRRRGGEG"
     gene            complement(9049..>9919)
                     /locus_tag="VEL74_13215"
     CDS             complement(9049..>9919)
                     /locus_tag="VEL74_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HYG63535.1"
                     /translation="VVAGIFFVSPELAMTETPRQGTLGEAALLLLFAYAGFENTAAPA
                     GEFKNPRRDVPFALMTQIVIVTLIYTAVQWVALGTLPGVAQSQTPLADSARLFLGPWG
                     GLLLTFGAAVSILGTNSNTVLAGPRYLFALAEDGFGPRALSRLHPRYRTPSVAILAQT
                     AIALPLALTGSTLVQETLNLPPALRGSFAKLAMLSIVARLATYFGTACAVPVLRRKFA
                     GVPTAVRLPGGPAIPILAGLVCIIFAASAKKENLIAGGIALLIGLLIYALRRPPEPSH
                     TEPADPIAPSTRL"
BASE COUNT         1471 a         3560 c         3457 g         1431 t
ORIGIN      
        1 ggggcggtcg gtccgctccc gcagacgatg gatctgcgcc cgcagctcgt cgggaccgat
       61 gcggacgccg gccaggatgc ccaatcctcc cgcccgcgcc acctccgccg ccagctccgg
      121 ccccgccacc tggcgcatgc ccgactgcag caccgggacg tcgatcccca ggaggtcggt
      181 cagaggcgtg cggagcggtc gcatggctct catactccca caaggccggt cccgcgcgac
      241 ctcaccagag tgcatgcgtt atgatgagag cctcaggagg aactcgccat gcgctctctc
      301 cagatccgag atatgcccga agacgtctac gaggccctgg cttcccgggc tcgatctgag
      361 catcgtagcc tggcacagca ggctatcgtg gagctgcgcc gaatccctga actgacagct
      421 cgggaacggc ggctccagag actcgaagag ttgaagaagc ggatcacgtc ggagcctccc
      481 cgacatctgc ccgtcccgcc agctgagctg atccgtgagg atcgcggtcg gtgacttcgg
      541 agatcgtgtt ggacgcctcg gcggctatcc atctcatcat ggccgccgaa cacgcctcgg
      601 acctggtgga gcgcctggaa agggccgctt cggtcgccgc tccggatctc ttcttctcgg
      661 aggtgggaaa cgctctgtgg aagtatgtcc agttcaggac tctcaccgtc gaaaaagctg
      721 tgacgcacct gcaggaagcc atggatctgg tggacggcat tgtccctacg caggaattga
      781 tccaggaggc cgtgatcgcc gcagcccggt atggtcatcc cgtctacgac atgctctatg
      841 cggtcctggc gcggcgccac ggcgcgacgg tcatcaccac tgataggtcg tttgcctcac
      901 tgttgcggcg catggaaatc gacacctact gtcctctcat gtaggccacc tctcccctct
      961 ttctaccctc ctccgcaagg agaggggact aatactcacg atctcaaaaa tcttcacctg
     1021 gactattgac atgtcaacta ttgccgtgta gagtctccac catgagcgag gagctgagcg
     1081 cgatcgcgcg ggagatccag cagcggcggc cctttcggac caagggccag gaggtggtcg
     1141 tgagcctgct ccgcacctcc gacgtcctgc gccgcttcgt gacccgggtg ctggagccgt
     1201 acgggatcac cccgcagcag tacaacgtgc tccgcatcct gcgcggtgcc ggcgaggacg
     1261 gctgccccac cctctccatc ggggagcgga tgatcgagga ggcgcccggc atcacccggc
     1321 tcatcgaccg gctcgagatc aaggggctgg tgcggcgcca gcgctgcgtc gaggaccgcc
     1381 ggcaggtgct ctgctggctc acctcggaag gacgcgccct ggtggaccgc atcgacgagc
     1441 ccatggacac cgccgacgac gaggccgtgg gcatgctgtc cagcgaggag cagaccaccc
     1501 tctgcgagct cctggacaag atccgcgcgg gtcatcgctg attttttttg gttgtacggt
     1561 tgactagacg actattgcca gatcatacaa aggagagaat gtcatgaagc gtgtactgct
     1621 caccgctgtt gcgctgtctt ccatcctgtc gctgccggtg caggcggccg aggtccataa
     1681 gatcgacccc acccactccg aggtgtcgtt ccagatccgc cacatggtca cccaggtgcg
     1741 gggcaacttc aacgagtacg agggcgtgat ccatctcgac ccggccaacc tggagggctc
     1801 caaggtcgac ctcaagatca aggccgccag catcgacacc aacctggccg accgcgacaa
     1861 gcacctgcgc gcgcccgact tcttcgacgt cgagaagtat ccggagatca ccttccgcag
     1921 cacgaagatc aagaagaccg gcaaggacac ctacgacgtc accggcaact tcaccatgcg
     1981 cggcgtcacc aagcagatca ccctgccggt gacctacctg ggctcggcca aggacccctg
     2041 gggcggccag cgcgccggct tcgagacctc gacgaccctc gaccgcaagg actacggcat
     2101 caactggaac accgcgctgg acaacggcgg cgtgatcttg ggtgacgacg tgaaggtgtc
     2161 gatcaacctg gagacgattc agcagaagga cgccaacgcc aagggcaagt gaccgcagct
     2221 ccttccggcg ggaccggtgt tcgcttcttc caccggtccc ccgctttttc ctacttctcc
     2281 ggccggctcg ccttggtgag gcgacaagct gaccggttct agataacggg gttctgcagt
     2341 acaggcgggc cgggggtggc gagaggggtg gagcgggtgt ggagggtggc tccggggggc
     2401 gagaggtgga acggcccgcc tcgttttctt gccagcgctt ctttccagcg gcgcgggtac
     2461 aatcccgggg cgttccggga gccccgacca tgagccacgc ccgccgcctc gtcctcttct
     2521 tcagcgtctc cctgctcctc gcgctgctcc tcgcgatccc gggcctcgcg gccaccgatt
     2581 tctcgtccct cgagaaggcg gtccagagcg acctcgccgc caccggcacg cccggcgccg
     2641 ccgtgatcgt agtgagcggc gaccgggtgg tgttcgccaa gggcttcggc actgccaacg
     2701 tagagatcgg tgagaccggc gctccggtca ccctcgacac cctgttccag gtcggctcgg
     2761 tgaccaagct gctgaccgcc acggccgtcg tcgcctacgc ccaggaggga aagctgcggc
     2821 tggacgcccc cctcggcacc tacgtcaagg ggctcgcgcc gcgcgtcgca gaggtcacgg
     2881 tcgcccaact cctctcccag acctcgggga tgcgcgacga gccggcggag tacggcccgc
     2941 acgacgagtc ggcgctgctg gcggcggccc gcgcctggaa ggactccgac cggccgctcc
     3001 ccccggggac ggcgttctcc tactcgaacc ccggcctggc cctcgccggg ctcgccttcc
     3061 aggaggcggc cggcaagccc ttcgccgacc tcatggacga gcgcctgttc acccccctcg
     3121 ggatgagcca cagcaccttc cgtcccaccg aggcgatgac ccggccgctc tccatgggac
     3181 acgacgccaa aggggagggc aagcccacgg tggtgcgccc gatggcggac gacgcccgct
     3241 tctggccggc cggcagcact ctctacacca gcgccaacga cctcgcgcgg ttcgtccggg
     3301 cgttcctgaa cggcggccgg ctggacggca ggccggtcct ctcccccgcc gtcctctccc
     3361 aggtcgcgac accccacgcc gacatcccag ccctgttcga ggacggccac tacggatacg
     3421 ggctgatgct gttccgggag aaggggctgc gcgtcgccga gcacgccggc accatgaccg
     3481 ggttctcggg gctcatccgc atggtgcccg agcaccactt cgggctgatc gtgctggcca
     3541 accgctccgg cgtccgcctc cgcgccacca cggacgaagc catgcggctc ctcctgccgg
     3601 tgaaagccga cgcgccgccg gcggagcgcc cgtcgctccc cgtctccgcc gaggagcgcg
     3661 cccgcctcac cggcttctac tccgcccgct ggcccatgga ggtcttcgag aaggagggcc
     3721 ggctcttcct gcgccgcttc gaccaggagc tggagatgac caaggtgggc gaccgccggt
     3781 tcgccatccg tccgcccggc gccgaccgcc cgcaggagct ggtcttcggc cccgatggcc
     3841 ccggcggcca tcctctctat ctccacatgg cgctctgggc gttcgcacgg gctgccggat
     3901 gaagaccaac gccgcccgca tcctcgacgc gctgggcatc ggctacgagc tgcgggagta
     3961 cgaggtggac cccgaggacc tcgcggcgga gaccgtcgcc cgcaaggtgg ggctccccgc
     4021 ggagcaggtg tggaagacgc tggtggcgcg cggtgaccgc accggcgtgc tcctcgccgt
     4081 ggtgccgggg gacgccgagc tggacccgaa ggcgctcgcc cgggcgaccg gcgaccgcaa
     4141 ggtggacacc gtgccgctgc gcgaggtgca gccgctcacc ggctacatcc gcggcggcgt
     4201 cacggccctg gggggcaaga aggactaccc ggtctgggtc gacgagacgg tggagctgta
     4261 cgatctgatc tcggtgtcgg ccggcgtccg gggcacccag atcctgctat cccccgccga
     4321 ctacctgcgc gccacccggg ggaaggtggc ccccatctcc cggccgaagg aactgtaacg
     4381 cggcggagcc cccacgccgg ccatagatga catagaattt ctacgagcgc accgaccatg
     4441 agccaggccg ccaccctgct gcccctccct ccggtcccgc ggcccgccgt ggtcagccgg
     4501 ccccggacgg tgcgcttgga gacctcggtg ccccgcctgc tccacgtcac taacggcgac
     4561 tgcgccgccg acctcctgcg gctctcggga atccccggca ccgtgacgct gaccgccgac
     4621 gtgctgcacg acggaccggt gccgccgccg accctggcac cggagcgctg gcgcaaggtg
     4681 cgcgcccgct ttctcgccga gagcggctac gaaggctacg acgagtgcct cgcccggctg
     4741 accgagtggg accgggcgct cgaggccttc cgctcctatg aagaggtggt gctctggttc
     4801 gagcacgacc tgttcgacca gctccacctg atccgcctgc tggactggtt cggccgccgc
     4861 gaggtgggct ggacccgcct gagcgtcgtc tgcatcggcg agtttccggg catccagcgc
     4921 ttccggggcc tggggcagct cgagccgatg gacttcacct cgctgctgga gctccgcaag
     4981 ccggtgaccg acgagcagat acggctggcg cgcaacgcct ggcgggtgtt ctgctcggcc
     5041 gagccgatcg gcctcgagct gctgctgcgg cgcgacacct cggcgctccc cttcctcgcc
     5101 ggcgcgctgc tccgccacct ggaggagttc ccgtcgacgc gcaacggcct gtcgcgcacc
     5161 gagcaccaag ggcttctggc ggtggccgag gtggccggag acggcggcgc ggcgacgttc
     5221 gccgaggtgt tccaggcggc ccaggacaag gaggagcgcg ccttcctggg ggatctgccc
     5281 ttcctgcaac gcctgcgcga gatggcctcc cggccgcgcc cgctgctccg catcgagccc
     5341 ggctccgacg attcgatggc ggcgctccag gtgacgatca cacccgcggg cctcgaggtg
     5401 ctcgacgggc gggaggactg ggtccacatc cacgggatcg accgctggct cggcggcgtc
     5461 cacctggtcg gcttcgagtc ccagtggcgc tgggatcccg accgccagcg cctcgccccc
     5521 acctgggccc ccgcccctcc ggccagcgcc tggccgtagg gctaccccgg ccccaccttc
     5581 ttcaacagct cggcgaagcg cggatcggcg cgcagcccgt cccagctcgg gtggacggcg
     5641 agcagggtca gcgtcgggga gcgctcctcg acagctttct ccagccagtc gaaggcccgg
     5701 tcctcgttcc ccacgaaggc ccagagggag gcgacggagg ctgagttgac ctgggagccc
     5761 tgggccgccg ccgcctccag gcgctggagg ttccagagat agaaggcgcg cagggcctcc
     5821 tccggctccc tcgtctccag ccccagccgc tcgatggccg cccgctctcc cggggtggcg
     5881 tccgcccgcg cccagcgcgt gctcgcgagg cgccaggcct ccctgtgcag gccctggagg
     5941 gtgtacgcct gctccatgca gaactgcgcc tcgccgaagc cgggcaccag ctccagcgtc
     6001 cgttggctgc gggcgaccgc ttcgtcgtat cggcgggcgc cgatggcggc ccagcccacg
     6061 tcgacgttgg cgcgcgcgga gagggggtcg aggtcgcgcg cccggtccac ctgatccagc
     6121 ccctcgtcgg cccggccgcg ggcgatgagg aagaagccgt agtcgtggcg cgcctcgggc
     6181 aggctcgggt tgagctccag ggcgcgccgg agctcccgct cggccgccgg ccagtcccag
     6241 cggtaccaga agaggatagc gccgaggacg gcgtgggcct cggcgaggcc ggagtccagg
     6301 gccacggccc ggcgcgccgc ggcctccgcg cgggggtagc cctgcctggg ggacagcccg
     6361 ccgctcatca ccagccggtg cagggcgtcg gccatggcgg cgtgcgcctc ggcgaaggcg
     6421 ggctcccggc ggaccgcccg ctcgtaggga tccaggctcc gcctcaggct ctcgtggtcg
     6481 ccgcggtggg cgaggtagcg gccgcgcagg tagtcgtccc aggcgcgggg gtcgggcggg
     6541 gtccggggct ggcgcgccga ctcggggagc agctccagct ggagcgcccg gaccacctcg
     6601 gctcccacct cggcctggac gtccagcacg tcgcgccggt cccggtcgta ggtctcggac
     6661 cagagctggg tacggtcgtc ggcgtggacg agctgagcgg tgatccgcac ccggtcgccg
     6721 gcccgccgga cgctcccttc gagcaggtag tcgacgccga gctcgtcgcc gagctcctcc
     6781 acgtcccggc gcgccccctt gtaggccatg accgaggtgc gggcgatcac ccccagccgc
     6841 tccggccgca cccgcccgag ctgggcgatg agctcgccgg tcaggccgtc gctccagtac
     6901 tcctcgggag ggccggcgct caggtcctcg aacgggagca cggcgatcat cagccgcggc
     6961 tccgtcccgc cgtcccggtg ccgccaggcg acgaaggcga tcaccagcag gaggccgagg
     7021 accgcggcca cggcccaggc gagcagccga gagggctggg agccgagacg gggcacctga
     7081 ggaaggggca gaggcgccgg cggagcctcg acggcttcct cgacgagctg ggggatcgcg
     7141 gcctttggga cacgctccac gggggcgatg aagcggtagc cgcggcgggg gagcgtctgg
     7201 acgaagcgcg ggctctgggc gtcgtccccc agggcggcgc ggatctggga aatacagaaa
     7261 ttgaggccgc gctcgaagtc gacgtaggtc tcttcccccc acacctggcg ccggatctcg
     7321 tcgcggctca ccggctcgcc ggcccgctgg gcgagcaggg cgagaatccg gaacggctgc
     7381 ggcgccagct tgaccgtctc cccgccccgg cgcaggtcgc cgctttcggg gtccagctcg
     7441 aaatcctcga aacgaaggcg ggtcttgggc tcaggagcgg ggggcgacaa caggggatac
     7501 ctcggtgacg acattatagg agggaaggct ggagggttcc gagactcgga gcggtctacg
     7561 cgcgagtcct caccccccgg ccccctctcc catctcccaa ccgccccccg ccgggagagg
     7621 gggagaaaga cagattaatg ctggtctccc cctctcccgg ggcaggggtg gacgggagat
     7681 gggagagggg gccggggggt gaggactccg ggcgcgaatg ccaccgctac ccctcccctc
     7741 cccgcctccg cagcatctcc accatctcct ccttgctcgc ctgagtggcc atggcgaggg
     7801 gtgtcagcac cccctgggcg gtcttggccc gggcattgac gtcggcgcca cggtccagga
     7861 gcagcgacgc catttcgagc tgacccgtgc gcgccgccag gtggagggcg gcctggcccc
     7921 tcttgtggct gttcacgtca gtcccggctt ggaggagcgc cttcatcacc tccagatggc
     7981 cgccgcccac caccgggaac caggtgaggg ggatgttgtg cgccccggtc tcccgcacgc
     8041 gcttcgggtc ctcccggagg agcgcgccga cccggtccag catccccatc atcgccgcgg
     8101 tgcagaggga gagcggcgcg ccgcgctcga gctggaactc cacgatctcc cggttgccca
     8161 cgtgggccgc cgcctccacc gccatctcgt cccaacgggc gcgggtgagg agcagtccgg
     8221 ggtgggaggc cagcagctcc cgcacgcgtg cgatgctgcc gtgcgacacc gccacgtact
     8281 cgtggatcag ggcgatgggc agcgggctcg acgtgtcctg ccggacgaag ggagcccctg
     8341 tcgccgtgta ggcgaagcgg gaggtggagt ggtcccgtgg caggtcccgg tggcggcgca
     8401 ggaggtccgc ggcctcccgg tcagcggcga gctggagggc cgtcttgccg tccggcgtcc
     8461 tggcctcggg gtccgccccg tcgtagatca gcgcctggat cacctcgatg ttgccggcgg
     8521 ccgccgcggc gtggagagga gagacgccgt ccgcctgccg ggcgttggga tcggcgccgt
     8581 tgaccaccat cgcgtccacc atcagctccg ccgtggcgag gtcggcgtgc tccgcggccc
     8641 agcgcagggg ggtgaacccg gggggatcgg tggagagggc gttggagtcc gcccccttcc
     8701 ccaccaggaa cgagaccatg gctccctgcc ccgcccgcac cgccgcgtgg agcgccgtgc
     8761 cgccgccggg gagggcgcgg tcgatgaggc cgggctcctt cgcgaggagc gcctccaccc
     8821 gctgggtgtc gccggaccgg accgcctcca gcagctccag ctccgcgggc ggcgtctggg
     8881 tttggatgtc agcggccaag gacagccctc cgggcagcgc gcagccggcg gcggccagcg
     8941 acagcgtctt gagaaaggtc gatcgcgtca tatccattcg tagcctcctg accccggaag
     9001 gtacggcacc cccggcggac cgggggtgag ggcgatgtta gagagagatt agaggcgggt
     9061 cgacggcgcg atggggtccg ccggctcggt gtgcgacggc tccggcgggc gccgcagggc
     9121 atagatgagg agcccgatca gcagggcgat gccgccggcg atcaggttct ccttcttggc
     9181 gctcgccgcg aagatgatgc agaccaggcc ggcgaggatc gggatcgccg gcccgccggg
     9241 caggcggacc gccgtcggca ccccggcgaa tttgcggcgc agcaccggca cggcgcacgc
     9301 cgtcccgaag taggtggcga ggcgcgccac gatggagagc atggcgagct tggcgaacga
     9361 gccccggagc gcggggggga ggttcagggt ctcctgcacc agcgtcgagc cggtgagcgc
     9421 cagcggcagg gcgatggcgg tctgggccag gatcgccacc gacggcgtgc ggtagcgcgg
     9481 gtggagccgc gagagcgccc gcggcccgaa gccgtcctcg gccagggcga acaggtagcg
     9541 cggcccggcc agcaccgtgt tgctgttggt ccccaggatc gacaccgccg caccgaaggt
     9601 cagcagcagc ccgccccagg ggccgaggaa gagccgcgcc gagtcggcga gcggcgtctg
     9661 ggactgcgcc acccccggca gagttcccag cgccacccac tgcaccgccg tgtagatcag
     9721 cgtcacgatg acgatctggg tcatcagggc gaagggcacg tcccgccgcg ggttcttgaa
     9781 ctcgcccgcc ggcgccgcgg tgttctcgaa cccggcgtag gcgaagagga ggagcagcgc
     9841 cgcctcgccc agcgtcccct gccgcggcgt ctcggtcatc gccagctcgg ggctgacgaa
     9901 gaagatgccg gcgaccacg
//
LOCUS       DASPJI010000485         7282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_715294, whole genome shotgun sequence.
ACCESSION   DASPJI010000485 DASPJI010000000
VERSION     DASPJI010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7282)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715294"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VEL74_13220"
     CDS             complement(<1..236)
                     /locus_tag="VEL74_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HYG63536.1"
                     /translation="MKRTKEISVGAGTLVIETGHLAKQANGSCTVRYGDTVVLVTACM
                     DAKPGLERDFLPLTVDYKEYTYAAGRIPGGFFKR"
     gene            complement(423..692)
                     /gene="rpsO"
                     /locus_tag="VEL74_13225"
     CDS             complement(423..692)
                     /gene="rpsO"
                     /locus_tag="VEL74_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HYG63537.1"
                     /translation="MAQTREIKEAIITDYRAHETDTGSTEVQVALLTSRISELTEHFK
                     THAKDHHGRRGLLKLVGRRRRLLDYLKGQDLERYRSTIERLGLRK"
     gene            complement(754..1752)
                     /gene="truB"
                     /locus_tag="VEL74_13230"
     CDS             complement(754..1752)
                     /gene="truB"
                     /locus_tag="VEL74_13230"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HYG63538.1"
                     /translation="MRDGLLLVDKDCGLTSHDVVQRVRRVLRQKKIGHCGTLDPDATG
                     LLLLTLGTATRLTRFLIRAPKVYEGTIRFGISTDTYDAAGQVVAEAPPEAVRALTREA
                     VEEAMRRFEGTIEQQPPPYSAKKVQGVKYYELARRGEEIPADTKEVTVFELSPQGALE
                     DGRIGFRLACSSGTYARGLAHDVGQALGCGAHLASLRRIKIGHFPVEDAVTLPRLEEA
                     VGKAAQAVSPESPDGEEAPEAPETPAMSEAAAGVGRAWIPFDEIPLPFDEVTADPQQE
                     NRIAHGQTVLVRELRGDEGDWVKLINRRREFIAVGTVVERIGTAGVGIVQPRVVFR"
     gene            complement(1756..2781)
                     /locus_tag="VEL74_13235"
     CDS             complement(1756..2781)
                     /locus_tag="VEL74_13235"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HYG63539.1"
                     /translation="MGGMKAPEDLLRKVRQGNRFLLTSHVNPDGDAIGSELGLARILR
                     KLGKGAVVWNRDATPTVYRPLPGSDRIHNGEEPPAGFPEKFDAIIVLECPSPDRTGLE
                     QHLSALPLINIDHHLGNQHYGSVNWVDSAAPAVGEMVYRLAQGLKVALDPETAACLYL
                     TLVTDTGGFRYSNATAAAFEAAASLVRDGAHPEQVAQWLYESQPVAVMRLLCQMLGTL
                     ELHEGGRVATARLTQEMFQRAGAEPGDSEGLIDHPRSIAGVEAVALVREREDGTHKVS
                     LRSRGEVDVEKIARHHGGGGHRNAAGFAMDGDGEEIRRQVAAALAAALSMPAVTEGSA
                     VPEPEAV"
     gene            complement(2759..3115)
                     /gene="rbfA"
                     /locus_tag="VEL74_13240"
     CDS             complement(2759..3115)
                     /gene="rbfA"
                     /locus_tag="VEL74_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011247069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HYG63540.1"
                     /translation="MSRRTGRVGDLIRAELSDLLLRHVQDPRIRLASVTEVDVTPDLR
                     RATVKVSVLGQDDEQRQETIEALRHARGFLRTELAHRLHMRATPELVFELDRGAEYSQ
                     RISDLLESLNGRDESS"
     gene            complement(3112..3408)
                     /locus_tag="VEL74_13245"
     CDS             complement(3112..3408)
                     /locus_tag="VEL74_13245"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HYG63541.1"
                     /translation="MIVGISVFELHLPSSRSLKDKRRVVKSLVDRIHQRYRVSVAETG
                     FQDLHQRSEIAIAAVVAGKEKEMDKLMEELRNLVESDPEIYLTHWEPQILEGES"
     gene            complement(3493..6549)
                     /gene="infB"
                     /locus_tag="VEL74_13250"
     CDS             complement(3493..6549)
                     /gene="infB"
                     /locus_tag="VEL74_13250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HYG63542.1"
                     /translation="MGKIRVQDLAKMMGIANQDLVFKLKSIGVRVEGDDAHIDTDIIQ
                     AILQGKKLPHPKEVILRDEQAPQPAPGRRPTAAAPPPPPARRPAANPLRPQRPRTLIQ
                     KVEPRIQTLPTMERPVTHETFAPEIEEAEAPSMAPAPETAEAPREEIAASAEPASQPA
                     APEAPVPAAPPAPEPAVPVQAPIAASAPAPVPSSRPSAPPSGPGGRPGGPPGRPSGPP
                     PSSGRPSGPGGPGGPGGRPSGPSGRPPYGGGPGGGRPSGPPSGGRPPYGGGPGGGRPS
                     GPGGPGGPGGRPSYGGGPGGPGGPGGQRRDQPYGGAGGGTGAPGGLGRRPGSPPSAPV
                     GGGYRTAAAPATDDRRRPGDRAAAEAARRKKGIKKPGPPGPEDEDLKVFKLEDLEGGD
                     EETLGTGTRRRRRAQRKEEERDERGKLLSFKKAPPTGPVMIGEGMTLREFAEKLGVKV
                     HDLIKALFSRGILANINQVIEPELAQKLADDLGVETMVVSFEEEVQLREELAGAGGGE
                     AEPSKGRVARAPVVTIMGHVDHGKTSLLDAIRSSKLTEAEFGGITQHIGAYHVDIRGR
                     NIVFLDTPGHEAFTLMRARGAKVTDIVVLVVAADDGVMPQTIEAIDHARAAKVPIVVA
                     INKVDKANANLDRVKRELADRGLQAEDWGGDTVMVPLSALKREGINDLLEMILLNADI
                     LELKANPELRAQGAVLEARKDVGRGIVATVLVQNGTLKVGDTFVSGNTWGKVRSMTDD
                     LGRRIPSAGPSTPVEVTGFSDVPDAGDLLQVVEDEQRARGIAEFRSEEHRRRGLTPVQ
                     GRMSLEQLFGRIQEGEVKELPVVLKADVQGSVEVLKEALTKTSTEKVKVQVLHSGVGA
                     ISTNDVLLASASKAIIIGFNVRPERSASELAEKEEVEIRLHTIIYELIDELKKAMTGL
                     LEPTFREVSSGRAEVRDTFKVPKIGTIAGCHVVDGVIPRSAAVRLLRDNRVIYEGKIA
                     SLRRFKDDASEVRAGFDCGIGLERFQDVKPGDVIEAFTREQVAPVL"
     gene            complement(6601..>7282)
                     /locus_tag="VEL74_13255"
     CDS             complement(6601..>7282)
                     /locus_tag="VEL74_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HYG63543.1"
                     /translation="QFVIGALAPAEVSSIVVDEEKHAMDVVVDEENLAVAIGRSGQNV
                     RLASELIGSRIDIKSESDVKDEVADALARMLQTAMSGSTAAAVPAAAADISSAPGVTP
                     ALQERLEEAGLTTPAALTEGGREALLAIDGVSEETADGLLAWAQEQQDRLAAEIGSTF
                     RQPEAASSSSMGDEDFMAALSRAFQESEQSRARFGGAADEEGGEDGAAENAAGDDDGD
                     SRSQETEQ"
BASE COUNT          915 a         2621 c         2570 g         1176 t
ORIGIN      
        1 tcccgcttga agaagccgcc cgggatgcgc ccggccgcgt aggtgtactc cttgtagtcg
       61 acggtcagcg gcaggaagtc ccgctcgagt ccgggcttgg cgtccatgca cgcggtgacc
      121 agcactaccg tgtcgccgta ccgcacggtg caggagccgt tggcctgctt ggccagatgg
      181 ccggtctcga tgacgagcgt acccgctccg acggagatct ctttggttcg cttcatagtt
      241 tgcttttctt tcttctctct tctgcctgtt gtctccggat tcaaccgtct ccggtcaccg
      301 cccggggaga gcggggaccg gagaggtccg gaggtccgaa aggtcggacg gtttccggtg
      361 ccgggggcgg gagggccgcc ggagcacgtc gggacggggt gctgcctggc tgacggggga
      421 gcttacttgc ggagcccgag gcgctcgatg gtgctgcgat agcgctcgag atcctgcccc
      481 ttgagatagt cgagcaggcg acggcggcgg cccaccagct tgagaaggcc gcggcggccg
      541 tggtggtcct tggcgtgtgt cttgaaatgc tcagtgagct ccgagatgcg gctggtcagc
      601 agcgcgacct gcacctcggt cgaaccggtg tcggtctcat gagccctgta gtcggtgatg
      661 atcgcttcct tgatctctct ggtttgcgcc aaagcaaact cctccttgca ctattctccg
      721 gtcggagtgt accacaacgt ccgtttcata tgttcagcgg aagaccaccc gcggctgcac
      781 gatgcccacg ccggcggtgc cgatgcgctc gacgaccgtg cccacggcga tgaactcgcg
      841 acggcggttg atcagcttca cccagtcccc ctcgtcgccg cggagctcgc ggacgagcac
      901 ggtctggccg tgcgcgatcc ggttctcctg ctgcgggtcg gcggtcacct cgtcgaacgg
      961 cagggggatc tcgtcgaacg ggatccaggc ccggcccacc cccgcggcgg cctcggacat
     1021 ggcaggtgtc tccggggcct ccggagcctc ctcgccgtcc gggctctccg gggagacggc
     1081 ctgcgccgcc ttccccacgg cctcctccag acgggggagc gtcacggcgt cctccaccgg
     1141 gaagtggccg atcttgatcc ggcgcagcga tgccaggtga gcgccacagc ccagggcctg
     1201 tcccacgtcg tgggccaggc cgcgcgcgta ggtgccggac gagcaggcca gccggaagcc
     1261 gatccggccg tcctcgagcg caccctgggg gctgagctcg aagaccgtca cctccttggt
     1321 gtcggccggg atctcctcgc cgcgccgggc gagctcgtag tacttgaccc cctgcacctt
     1381 cttggcggag taggggggcg gctgctgctc gatggtcccc tcgaagcgcc gcatcgcctc
     1441 ttcgaccgcc tcgcgggtga gggcccgcac cgcctcgggc ggcgcctcgg ccaccacctg
     1501 cccggcggcg tcgtaggtgt cggtgctgat cccgaagcgg atcgtcccct cgtagacctt
     1561 gggggcgcgg atcaggaacc gggtcaggcg cgtcgccgtg cccagggtga ggagcagcag
     1621 gcccgtggcg tcgggatcca gcgtcccgca gtgcccgatc ttcttctgtc tgaggacacg
     1681 gcggaccctc tgcacgacat cgtgcgaagt caagccgcaa tccttgtcga ccagcagcag
     1741 cccgtcccgc atcgtctaga ccgcctccgg ctccggcacc gccgagccct cggtcaccgc
     1801 cggcatcgac agcgcggcgg cgagggccgc cgccacctgc cggcggatct cctcgccgtc
     1861 cccgtccatc gcgaagccgg cggcgttccg gtgcccgccg ccgccgtggt gacgggcgat
     1921 cttctccacg tccacctcgc cgcggctgcg cagcgacacc ttgtgcgtgc cgtcctcgcg
     1981 ctcgcgcacc agcgccacgg cctcgacgcc ggcgatggag cgcgggtggt cgatgagccc
     2041 ctcggagtcg cccggctcgg cgccggcgcg ctggaacatc tcctgggtca ggcgcgccgt
     2101 ggccacccgt cccccctcgt ggagctcgag cgtccccagc atctggcaga gcaggcgcat
     2161 cacggccacc ggctggctct cgtagagcca ctgcgccacc tgctccgggt gggcgccgtc
     2221 gcgcaccagc gaggcggcgg cctcgaaggc cgccgccgtg gcgttggagt agcggaagcc
     2281 gccggtgtcg gtcaccaggg tgaggtagag gcaggccgcc gtctccgggt cgagcgccac
     2341 cttgagcccc tgggccagcc gatagaccat ctcgcccacc gccggcgcgg cggagtccac
     2401 ccagttgacc gagccgtagt gctggttgcc caagtggtgg tcgatgttga tcagcggcag
     2461 cgccgacagg tgctgctcca ggccggtgcg gtccgggctc gggcactcca gcacgatgat
     2521 ggcgtcgaac ttctcgggga agccggccgg cggctcctca ccgttgtgga tgcggtcgct
     2581 gccgggcagc ggccggtaca cggtcggcgt cgcgtcccgg ttccacacca ccgccccctt
     2641 gccgagcttg cggaggatgc gggccaggcc cagctccgag ccgatggcgt cgccgtcggg
     2701 gttcacgtgg ctggtcagca ggaaacggtt gccctgccgg actttgcgca gcaggtcttc
     2761 aggagctttc atcccgccca tttaggctct ccaagaggtc tgagattctc tggctgtact
     2821 cggcgccccg gtcgagctcg aacaccagct cgggggtcgc ccgcatgtgc aggcggtgcg
     2881 ccagctcggt gcgcaggaag ccccgggcgt ggcgcagggc ctcgatggtc tcctggcgct
     2941 gctcgtcgtc ctggccgagg accgacacct tcacggtggc gcggcgcagg tccggggtca
     3001 cgtcgacctc ggtcacgctc gccagccgga tccgcgggtc ctggacatgc cggagcagca
     3061 ggtccgacag ctcggcgcgg atcaggtccc ccacgcgtcc ggtgcgccgg ctcatgactc
     3121 cccctccagg atctgcggct cccagtgggt gagatagatc tcgggatcgc tctccaccag
     3181 gttccgaagc tcttccatca acttgtccat ctccttctcc ttccccgcca cgacggccgc
     3241 gatcgcgatc tccgaccgct ggtggagatc ctggaacccg gtctccgcca ccgacacgcg
     3301 gtagcgctgg tggatgcggt cgaccagcga tttgacgacc cgccgcttgt ccttgaggct
     3361 gcggctggaa ggaaggtgca gctcgaaaac ggaaatcccg acgatcatcg ttcgaggttc
     3421 ggccccccgc ggcagcgccc ccggggaccg cgggtccccg gagacgtccg gtgggggagg
     3481 aaaggggccg gtctacagga ccggcgccac ctgctcgcgg gtgaaggctt cgatcacgtc
     3541 gcccggcttg acgtcctgga aacgctccag gccgatgccg cagtcgaagc cggcgcggac
     3601 ctccgaggcg tcgtccttga accggcgcag cgacgcgatc ttgccttcgt agatcacccg
     3661 gttgtcgcgc agcaggcgga cggcggcgct ccgcgggatc accccgtcga ccacgtgaca
     3721 gccggcgatg gtgccgatct tcggcacctt gaaggtgtcg cggacctcgg cgcggccgct
     3781 ggacacctcg cggaaggtgg gctccagcag tccggtcatc gccttcttga gctcgtcgat
     3841 gagctcgtag atgatcgtgt gcagccggat ctccacctct tccttctcgg ccagctcgga
     3901 ggcgctgcgc tccgggcgga cgttgaagcc gatgatgatc gccttggagg ccgaggcgag
     3961 cagcacgtcg ttggtggaga tggcgccgac gccggagtgg agcacctgca ccttgacctt
     4021 ctccgtgctg gtcttggtga gggcctcctt gaggacctcc accgagccct gcacgtcggc
     4081 cttgaggacc accggcagct ccttgacctc gccctcctgg atgcggccga agagctgctc
     4141 cagggacatc cgcccctgga ccggcgtaag gccgcgccgc cggtgctcct cgctgcggaa
     4201 ctcggcgatg ccgcgcgccc gctgctcgtc ctcgactacc tgcagcaggt cgccggcgtc
     4261 cggcacgtcg ctgaacccgg tgacctccac cggcgtcgac gggcccgccg acgggatgcg
     4321 gcgcccgagg tcgtcggtca tcgaccgcac cttgccccag gtgttgcccg agacgaaggt
     4381 gtcgcccacc ttgagggtgc cgttctgcac cagcacggtg gcgacgatgc cgcggccgac
     4441 gtccttgcgc gcctcgagca ccgcgccctg ggcgcgcagc tcggggttgg ccttgagctc
     4501 caggatgtcc gcgttgagca ggatcatctc cagcaggtcg ttgatgccct cgcgcttcaa
     4561 cgcggacagc ggcaccatga ccgtgtcgcc gccccagtcc tcggcctgca ggccgcggtc
     4621 ggccagctcg cgcttgaccc ggtcgaggtt ggcgttggcc ttgtcgacct tgttgatggc
     4681 gaccacgatc ggcaccttgg cggcgcgggc gtggtcgatc gcctcgatcg tctgcggcat
     4741 gacgccgtcg tcggcggcca ccaccaggac gacgatgtcg gttaccttgg cgccgcgcgc
     4801 ccgcatcagg gtgaacgcct cgtggcccgg ggtatcgagg aagacgatgt tgcggccgcg
     4861 gatgtccacg tggtaggcgc cgatgtgctg ggtgatgccg ccgaactcgg cctccgtcag
     4921 cttggaggag cggatggcgt ccagcagcga cgtcttgccg tggtcgacgt ggcccatgat
     4981 ggtgaccacc ggggcgcggg ccacacggcc cttggagggc tcggcctcgc cgccgccggc
     5041 accggccagc tcttcacgca actgcacctc ctcctcgaag gagaccacca tggtctccac
     5101 ccccaggtcg tcggcgagct tctgcgccag ctcgggctcg atcacctggt tgatgttggc
     5161 caggatgccg cggctgaaga gcgccttgat caggtcgtgc accttcaccc cgagcttctc
     5221 ggcgaactcg cgcagggtca tgccctcgcc gatcatcacc gggccggtcg gaggggcctt
     5281 cttgaacgac agcagcttgc cgcgctcgtc ccgctcctcc tccttgcgct gggcgcggcg
     5341 gcgccggcgg gtgcccgtgc cgagcgtctc ctcgtcgccg ccctccaggt cctccagctt
     5401 gaataccttc agatcctcgt cctccggccc cggaggaccg ggcttcttga tccccttctt
     5461 gcgcctggcg gcctcggccg cggcgcggtc gccggggcgg cgccggtcgt cggtcgccgg
     5521 cgcggcggcg gtacggtacc cgccgcccac cggggcggag ggggggctgc cgggacgccg
     5581 gccgagcccg ccgggcgcgc cggtgcctcc accggcgccg ccgtacggct ggtcacggcg
     5641 ttggccgccg ggtccgccgg gtccaccggg cccgccgcca taggacggcc tgccacccgg
     5701 tccacccgga ccgcccggtc cggacggacg gccaccgccg ggcccgccgc catagggcgg
     5761 cctgccgccg gagggcggtc cggaaggacg gccaccaccc gggccaccgc cgtagggcgg
     5821 gcggccggag gggccggagg gcctgccacc cggtccgccg gggccacccg gtccggaagg
     5881 ccggccggaa gacgggggcg gtccggaagg acggcccgga gggccacccg ggcggccgcc
     5941 aggaccggac ggcggcgcgg acggccggct cgaaggcacc ggcgcgggcg cggacgccgc
     6001 gatcggggcc tggaccggta cggccggctc gggcgccgga ggcgctgccg gaacgggcgc
     6061 ctcgggagcc gccggctggg aggccggctc ggcgctcgcg gcgatctcct cgcgcggcgc
     6121 ctcggcggtc tccggcgcgg gcgccatcga cggagcctcg gcctcctcga tctccggagc
     6181 gaaggtctcg tgcgtcaccg gccgctccat ggtgggcagc gtctggatgc gcggctccac
     6241 cttctggatc agcgtgcggg ggcgctgcgg ccgcaacggg ttcgccgccg gacggcgggc
     6301 cggaggcggc ggcggcgcgg ccgccgtggg gcggcggccg ggagccggct gaggggcctg
     6361 ctcgtcgcgc aggatgacct ctttcggatg cgggagcttc ttgccctgca ggatcgcctg
     6421 gatgatgtcc gtgtcgatgt gggcgtcatc accttcgacg cgcacgccga tggacttcag
     6481 cttaaagacg aggtcctggt tggcgatccc catcatcttt gcgaggtcct gtactcgaat
     6541 ctttcccata gtctcctcag aactcctgtt cgaacccccg ggccgcgtgc cgccggttta
     6601 ctactgctcg gtctcttggg aacggctgtc gccgtcgtcg tcgccggccg cgttctccgc
     6661 cgcgccgtcc tcgccgccct cttcgtcggc agcgccgccg aaccgcgccc gcgactgctc
     6721 gctctcctgg aaggcccggc tgagcgccgc catgaagtcc tcgtcgccca tggagctgga
     6781 cgacgcggcc tcgggctggc ggaaggtcga gccgatctcg gcggcgagcc ggtcctgctg
     6841 ctcctgggcc caggcgagca ggccgtcggc ggtctcctcg gagacgccgt cgatggcgag
     6901 gagcgcctcg cggcccccct cggtgagcgc cgcgggcgtg gtcaggccgg cctcctccag
     6961 gcgctcctgg agcgccggcg tgacgcccgg cgccgacgag atgtcggcgg cggcggcggg
     7021 gacggccgcg gccgtcgagc ccgacatggc ggtctggagc attcgcgcca gcgcgtcggc
     7081 cacctcgtcc ttgacgtcgc tctcgctctt gatgtcgatg cgcgagccga tcagctcgga
     7141 ggcgaggcgg acgttctggc cgctgcggcc gatggccacg gccaggttct cctcgtccac
     7201 cacgacgtcc atcgcgtgct tctcctcgtc gacgacgatg gagctgacct cggccggcgc
     7261 cagcgcgccg atcacgaact gc
//
LOCUS       DASPJI010000486         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_376152, whole genome shotgun sequence.
ACCESSION   DASPJI010000486 DASPJI010000000
VERSION     DASPJI010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376152"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2615)
                     /locus_tag="VEL74_13260"
     CDS             complement(<1..>2615)
                     /locus_tag="VEL74_13260"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG63544.1"
                     /translation="VVLPELPLTPNGKVDRRALPDPAATIAVRQGGLAAPTDAVEEII
                     AGIWAEVLALPAVGTDQDFFEIGGHSLLATQVVSRMTEALQVMIPLPRFFEEPTVAEQ
                     AAFIRAARRAERQVDDLLPIVPVPRETGIPLSFAQQRLWFIDQLEPGSAAYNIPAAVR
                     LAGRLEVAVLEWALLEVVRRHEALRTTFGAREGEPFQRIAPEPELPLPLVDLAELPVG
                     SCRQEAERLAGAEASRPFDLAAGPLVRWTLLRLDAEEHLALVTMHHIVSDGWSAAIFL
                     RELAALYDTCRTAEAAEDAVLAELPVQYADFAVWQRRWLTGEVLERQIGHWRHALAGV
                     PPRLELTTDRPRPVVQSYRGATLPVLLPHPLAAAIAALGRQLGATPFMMLLAAFQTLL
                     SRLTGQEDVVVGSPIAGRNRREIEGLIGFFVNTLVLRADLAGEPVFRQLVSRLRAAAL
                     AAYEHQDLPFERLVEELAPERSLAHSPLFQVVFALQNMPASSFELPGLALEALPAAGD
                     GVAKFDLTFTLGESAAGFSGSIAYSTDLFDGTTVARWMEHLRTLLEDLVAHPERRVSE
                     LALLSPAERHQALHRSGTMEAPAEVFLLHRRFREQAAARPDAVAVTWEESNLSYGELR
                     DRAGRLARRLRALGVGPDVRVGVCLDRSAELVTALLAVLEAGGAYVPLDPAYPEERLA
                     LLVADAGLAVVVTTAAAAGRLPATGVERLLLEEAGADGAAWRDDAAAPESLAYVIYTS
                     GSTGRPKGVQVSHAAASRLLTATHPWFGFGPGDVWSLFHSFSFDFSVWEIWGALLHGG
                     RLVVVPFWAGREPERFLDLLAREEVTVLNQTPSSFRTLARAVEEGSSPSSLRWVVFGG
                     EALDFANLAPWFARHG"
BASE COUNT          433 a          935 c          954 g          293 t
ORIGIN      
        1 ccgtggcgcg cgaaccaggg agcgaggttg gcgaaatcca gcgcctcgcc gccgaagacc
       61 acccagcgca gagaagaggg ggacgagcct tcctccacgg cccgcgccag cgtccggaag
      121 ctcgagggcg tctggttgag caccgtcacc tcctcgcgcg cgaggaggtc caggaagcgc
      181 tccggctcgc ggccggccca gaagggcacg accaccaggc ggccgccgtg aagcagggcg
      241 ccccagatct cccagaccga gaagtcgaag gagaaggagt ggaacaggct ccagacgtcg
      301 cccgggccga agccgaacca gggatgggtg gcggtcagca gccgcgacgc ggcggcatgg
      361 ctgacctgga cacccttggg cctgcccgtc gagccggagg tgtagatgac gtaggcgaga
      421 ctctcgggtg ccgccgcgtc gtcccgccag gcggcaccgt cagcgcccgc ctcctcgagg
      481 agcagccgtt cgacccctgt ggccggcaag cggccggccg ccgccgcggt ggtgacgacg
      541 accgcgagac cggcgtccgc caccaggagc gccagccgct cctcggggta ggccggatcg
      601 agcgggacat aggccccgcc cgcctcgagg acagccagga gggcggttac cagctcggcc
      661 gaccgatcca ggcagacccc cacccgcacg tcagggccga cccccagcgc ccgcagccgg
      721 cgggcgagcc ggccggcccg gtcacggagc tctccatagc tgagattcga ctcctcccag
      781 gtgacagcca cggcatccgg cctggccgcg gcctgctccc ggaaccgccg gtggaggagg
      841 aagacctcgg ccggggcctc catggtgcca ctgcggtgga gagcctggtg gcgctccgcc
      901 ggggaaagca gggccagctc ggagacccgg cgttcgggat gagccaccag atcctccagc
      961 aaggtgcgga ggtgctccat ccagcgcgcc acggtggtgc cgtcgaacag atcggtgctg
     1021 taggcgatgg acccggagaa gccggccgcg ctctcgccca gggtgaaggt gaggtcgaac
     1081 ttcgccacac cgtcgccagc ggcgggcagg gcctcgaggg ccaatccggg aagctcgaag
     1141 ctcgacgccg gcatgttctg cagcgcgaac accacctgga agagcggcga gtgggcgagg
     1201 ctgcgctcgg gcgccagctc ctccaccaat cgctcgaacg gcaggtcctg gtgctcgtag
     1261 gccgccagcg ccgccgcacg cagccgggag acgagctgac ggaacaccgg ttcgcccgcg
     1321 agatcggcac gcagcaccag ggtgttgacg aagaagccga tcagcccttc gatctcgcgc
     1381 cggttccggc cggcaatggg cgagcccacc accacgtcct cctggccggt gaggcgggag
     1441 agcagggtct ggaaagcggc caggagcatc atgaacggcg tcgccccgag ttgccggccg
     1501 agtgcggcga tggccgccgc cagggggtgc ggcaggagca ccggcagcgt cgcgccgcga
     1561 tagctctgga ccaccgggcg cggccggtcg gtggtcagct ccaaccgcgg aggcacaccg
     1621 gcgagggcgt ggcgccagtg gccgatctgc cgctccagca cctcgccggt gagccagcgg
     1681 cgctgccaga cggcgaagtc ggcgtactgc acgggcagct cggcgagcac cgcgtcctcg
     1741 gcagcctcag cggtgcggca ggtgtcgtag agggccgcca gctcccgcag gaagatcgcg
     1801 gccgaccatc cgtcggagac gatgtggtgc atggtgacca gcgccagatg ctcctccgca
     1861 tcgagccgca gcagcgtcca gcgcaccaac ggcccggccg ccagatcgaa cggacgcgac
     1921 gcctccgctc cggccagacg ctcggcctcc tgccggcacg agcccaccgg cagctccgcc
     1981 aagtcgacca gggggagcgg cagctccggc tccggtgcga tgcgctggaa cggctccccc
     2041 tccctcgcgc cgaaggtcgt ccgcaacgcc tcgtgacggc gcaccacctc caggagcgcc
     2101 cactccagga ccgcgacctc gaggcggccg gccaggcgca ccgccgccgg gatgttgtag
     2161 gcggcgctcc ccggctcgag ctggtcgatg aaccagagcc gctgctgcgc gaacgagagg
     2221 ggaatcccag tctcccgcgg tacgggcacg atggggagga ggtcgtccac ctgccgttcc
     2281 gcgcggcggg cggcgcggat gaaagcggcc tgctccgcaa cggtgggctc ctcgaaaaag
     2341 cgcggcagcg gaatcatcac ctggagggcc tcagtcatgc gcgagacgac ctgggtcgcc
     2401 aacagggagt ggccgccgat ctcgaagaag tcctggtcgg tccccacggc gggcagggcc
     2461 agcacctcgg cccagatgcc ggcgatgatc tcctccaccg cgtccgtggg agcggcgagc
     2521 ccgccttgcc gcaccgcgat cgtagccgcg ggatcgggca gagcgcggcg gtcgaccttg
     2581 ccattggggg tgagcggcag ctccggcagg acgac
//
LOCUS       DASPJI010000487         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_28969, whole genome shotgun sequence.
ACCESSION   DASPJI010000487 DASPJI010000000
VERSION     DASPJI010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28969"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VEL74_13265"
     CDS             complement(<1..317)
                     /locus_tag="VEL74_13265"
                     /inference="COORDINATES: protein motif:HMM:NF040068.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63545.1"
                     /translation="MKKNAHDWTPHLIDERPVQSFPRHGSYRLALVYANTYHVGMSSL
                     GFQRVYELVHQRPDWGCERFFMDGAGMPLSVESHRPLDDFGCVAFSVSFEEDYVHLLQ
                     MLDR"
     gene            complement(351..1469)
                     /locus_tag="VEL74_13270"
     CDS             complement(351..1469)
                     /locus_tag="VEL74_13270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYG63546.1"
                     /translation="MALAADILIVGGGPAGLATAIRARLAGMRVLLLERFQPPVDKAC
                     GEGLMPDAVARLREIGVELPPRQGVPFRGIRYFDGDLMAEGVFPHAGGLGVRRLHLHD
                     ALVRRAGEVGVEIHWGRGVRGLLREGVETDDGPVLARWVVGADGLHSRVRSWAGLDGG
                     AGPIHRFGVRRHYALPPWSDFVEVYWASGSPGCEAYVTPVTPDEIGVALLWSGGKSRF
                     DDLLQRFPVLRGRLAGAPAASRDRGAGPLHQQVRAVCRGNVALVGDAAGYLDAITGEG
                     LAVALHQSAALAEALRAGDLALYAAAHRRIARLPDFMTSLVLSLERRPGLRRRAVKAL
                     AAEPALFSRLLGIHGRTLPPRRLGLDGALRLAWRLVAA"
     gene            complement(1453..>2223)
                     /locus_tag="VEL74_13275"
     CDS             complement(1453..>2223)
                     /locus_tag="VEL74_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYG63547.1"
                     /translation="SDLGSSFRSGCFDRDALTGEGRARERSGGREIVSVGRPVPGFRL
                     RVVDEAGSDLPEGQVGRVLIQGPSLMEGYLGAPAATARAFADGWLDTGDLGFLHKEEL
                     YLTGRAKDVVILRGRNHAPEEIERSVEGVPGVRTGCAVAVSWLPEGAPGEVLALLVEA
                     ARGTGPVERAALPGACREAVLGATGLAVDEVVVLEPGTLPRTSSGKLRRQEALRLHLA
                     GELRPPDPVTPLRIAGAVARSGLAYARLRWGKHSGPRG"
BASE COUNT          324 a          798 c          861 g          240 t
ORIGIN      
        1 cggtccagca tctgaaggag gtggacgtag tcctcctcga aggagaccga gaaggcgacg
       61 cagccgaagt cgtcgagcgg ccggtggctc tcgacggaga gcggcatgcc ggcgccgtcc
      121 atgaagaagc gctcgcagcc ccagtccggc cgctggtgca ccagctcgta aacccgctgg
      181 aagccgaggc tcgacatccc cacgtggtag gtgttggcgt agaccagcgc cagccggtag
      241 ctgccgtggc gcgggaagct ctgcaccggc cgctcgtcga tgaggtgtgg ggtccagtcg
      301 tgggcgttct tcttcatggc caagaggtta caaccatccc tggaggtagt ttatgccgcc
      361 accagccgcc aggcgagccg gagggcgccg tccaggccca ggcggcgggg gggcagggtg
      421 cggccgtgga tgcccaggag ccgcgagaag agggccggct cggcggccag cgccttgacc
      481 gcgcgccgcc gcagccccgg ccggcgctcc agggagagca ccagcgaggt catgaagtcg
      541 gggaggcggg cgatgcggcg gtgggcggcg gcatagaggg cgaggtcccc ggcccggagc
      601 gcctcggcga gggcggccga ctggtggagg gcgaccgcca gcccctcgcc ggtgatggcg
      661 tcgaggtagc cggcggcgtc cccgaccagg gcgacgttgc cgcggcagac cgcccgcacc
      721 tgctgatgca gcggcccggc gccgcggtcg cgggaggcgg cgggggcgcc ggcgaggcgg
      781 ccgcggagga cggggaagcg ctggagcagg tcgtcgaagc ggctcttgcc gccgctccag
      841 agcagggcga cgccgatctc gtccggggtc acgggcgtca cataggcctc gcagcccggg
      901 gagcccgagg cccaatagac ctccacgaag tcgctccagg ggggcagcgc gtagtggcgg
      961 cggacaccga agcggtggat cggaccggcg ccgccgtcca ggccggccca ggagcggacc
     1021 cgggaatgga ggccgtcggc gccgaccacc cagcgggcga ggacggggcc gtcgtcggtc
     1081 tcgacacctt cccgaaggag cccccggacc ccgcggcccc agtggatctc cacccccacc
     1141 tctccggcgc ggcggaccag ggcgtcgtgg aggtggaggc ggcggacgcc gaggcccccg
     1201 gcgtgaggga agaccccctc cgccatcagg tcgccgtcga agtagcggat gccgcggaag
     1261 gggaccccct gcctgggggg tagctcgacg ccgatctcgc gcaggcgggc gacggcgtcg
     1321 ggcatcagcc cctcgccgca ggccttgtcg accggaggct ggaagcgctc gaggaggagg
     1381 acccgcatcc cggcgaggcg ggcgcggatg gcggtggcga ggccggcggg gccgccgcct
     1441 actataagga tgtcagccgc gagggccact gtgcttcccc cagcgcaggc gggcataggc
     1501 gagtcccgag cgggcgacgg ctccggcgat gcggagcggc gtcaccggat cagggggccg
     1561 cagctcgccg gcgaggtgga gccgcagggc ctcctgccgc cgcagcttgc ccgacgaggt
     1621 gcgggggagc gtccccggct cgaggaccac cacctcgtcg acggcaagac cggtggcacc
     1681 gaggaccgcc tcgcggcagg cgccggggag cgccgccctc tcgaccggcc cggtcccccg
     1741 cgccgcttcg accagcagcg ccagcacctc gccgggggcg ccttcgggga gccagctcac
     1801 ggccacggca cagccggtgc gcacccccgg caccccctcg accgaccgct cgatctcctc
     1861 gggggcatgg ttgcggcccc gcaggatcac cacgtccttc gcccggccgg tgaggtagag
     1921 ctcttcctta tggagaaagc cgaggtcgcc ggtgtcgagc cagccgtccg caaaggcgcg
     1981 ggctgtggcg gcgggcgctc ccaggtagcc ctccatgagg gacggcccct ggatcagcac
     2041 cctcccgacc tggccctcgg gcagatcgga cccggcctcg tccaccaccc gcagccggaa
     2101 ccccggcacc ggccggccga cggagacgat ctcccggccg ccgctgcgct cccgggccct
     2161 cccctcgcca gtcagggcgt cgcggtcgaa gcagccgctg cggaacgaag agccgaggtc
     2221 cga
//
LOCUS       DASPJI010000488         2555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_775539, whole genome shotgun sequence.
ACCESSION   DASPJI010000488 DASPJI010000000
VERSION     DASPJI010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775539"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1783
                     /locus_tag="VEL74_13280"
     CDS             <1..1783
                     /locus_tag="VEL74_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63548.1"
                     /translation="PTTPPTTPPTTPPTTPPAQGQGQGQGGQGQGQTQPAHSGTQVAV
                     TADLSYSMEKLEESGAIKIDIIRQQEGTSVDEMQRQALDLLKESILNEFFQPAMTAAP
                     AAGGIAPALGAAQQAQQLMAGSGQTNTGVAGAGTRVEVGFQLQFKKEEELQTADYDFS
                     VIQPETRTHAPNGFFSALLRGTEQAEHIREIDLDDPFFKILDVDVSTTADFDGLDLKT
                     IVVELQYGGTVESPRVTGTATFTKGDNAPRHFQAFRDLDDFSYRYRVAYKFGQAEGIG
                     AKRQEYQTPWRSTTSRALVVHPPEDVAMLRVFVETGVVDWDIIEKIDTVLTYDDPAHG
                     FHAERRFLLDSTSQRQPWLVRLEDPARAAYQVKSVWHLKDESEIQGPVETRRGSQFFA
                     GDPFIDRLPIVIQPLVDRNNVLRVEVELHYEDEANGLDVRKNVEVAGPDFRPVTVTLP
                     IVDRGRREYSYRVILIKANGGTEMQLPQRTEQLSILVSEGGIYFDVNVTLLGATGMAQ
                     NGVDAIQVDLRSEPLDGELQRVESHLFEPGAEAKFTKRLLLRSDRPRRFEFRTTVFSS
                     ANAPKESDWTLHENPILPLQVTRLLNA"
     gene            1797..>2555
                     /locus_tag="VEL74_13285"
     CDS             1797..>2555
                     /locus_tag="VEL74_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63549.1"
                     /translation="MIDLGRAPFHAEGVTLFPDHADPKRFHYLPDGPRLRFGPPEGDQ
                     EGEPELSLLKYRLDPALHTELGAGLLSLTVDLGVDEERLERLRGRLAARAGVSGPVLS
                     PVAADSGSCELVLIDRSSTDETAATGFGLVARILGAATPSLYGDNAATFMAVLSAEGM
                     ALAEGALRGGGLPAGVVYTLRTTGLRPALRAEITARWRDVYEFYENRFHGGKLLLAVD
                     IGPTIEELVQNELLSIRVDELVPAEERSAVLDRAI"
BASE COUNT          410 a          974 c          828 g          343 t
ORIGIN      
        1 tcccacgact cctccgacca cgccccccac gacgcccccc acgacaccgc cggcccaggg
       61 tcagggccag gggcagggcg gccagggaca gggacagacc cagccggccc actcgggcac
      121 tcaggtcgcc gtcaccgccg acctctccta ctcgatggag aagctcgagg agagcggcgc
      181 catcaagatc gacatcatcc gccagcagga aggcacctcg gtcgacgaga tgcagcgcca
      241 ggccctcgac ctgctcaagg agtcgatcct caacgagttc ttccagcctg cgatgaccgc
      301 cgcgccggcg gccggaggca tcgccccggc cctgggggcg gcccagcagg cgcagcagct
      361 catggccggc tccggccaga ccaacaccgg cgtggccggt gccggcaccc gcgtcgaggt
      421 cggcttccag ctccagttca agaaagagga ggagctgcag accgccgact acgacttcag
      481 cgtcatccag cccgagaccc gcacccacgc gccgaacggc ttcttctccg ccctgctgcg
      541 cggcaccgag caggccgagc acatccgcga gatcgacctc gacgacccgt tcttcaagat
      601 cctcgacgtc gacgtcagca ccacggccga cttcgacggg ctggacttga agaccatcgt
      661 ggtggagctc cagtacggcg gcaccgtcga gtcgccgcgg gtcaccggca ccgccacctt
      721 caccaagggg gacaacgcgc cccgtcactt ccaggccttc cgcgacctcg acgacttctc
      781 ctaccgctac cgggtcgcct acaagttcgg ccaggccgag gggatcgggg cgaagcgcca
      841 ggagtaccag acgccctggc gctccaccac cagccgcgcc ctggtggtcc accctcccga
      901 ggacgtggcc atgctccgcg tcttcgtcga gacgggggtg gtcgactggg acatcatcga
      961 gaagatcgac accgtcctca cctatgacga cccggcccac ggcttccacg ccgagcgccg
     1021 cttcctgctc gactccacct cccagcgcca gccctggctg gtgcggctgg aggacccggc
     1081 ccgcgccgcc taccaggtga agagcgtctg gcacctcaag gacgagagcg agatccaggg
     1141 gccggtggag acccggcgcg gctcccagtt cttcgccggc gatcccttca tcgaccggct
     1201 gcccatcgtc atccagccgc tggtggaccg caacaacgtg ctgcgcgtcg aggtcgagct
     1261 gcactacgag gacgaggcga acggcctgga cgtccgcaag aacgtcgagg tggcgggccc
     1321 ggacttcagg cccgtcaccg tcaccctgcc catcgtcgac cgcgggcgcc gggagtactc
     1381 ctaccgggtg atcctcatca aggcgaacgg cggcaccgag atgcagctcc cccagcgcac
     1441 cgagcagctc tcgatcctcg tcagcgaggg ggggatctac ttcgacgtca acgtcaccct
     1501 gctcggcgcc accggcatgg cccagaacgg cgtcgacgcc atccaggtcg acctgcgcag
     1561 cgagccgctc gacggcgagc tgcagcgggt ggagagccac ctcttcgagc ccggcgccga
     1621 ggccaagttc accaagcggc tgctgctgcg ctccgaccgg ccgcggcgct tcgagttccg
     1681 caccacggtc ttctcctcgg cgaacgcgcc gaaggagagc gactggacgc tgcacgagaa
     1741 ccccatcctg ccgctccagg tgacccgcct gctcaacgcc tgagaggaga cgcccgatga
     1801 tcgatctcgg ccgcgcgccg ttccacgccg agggggtgac cctcttcccc gaccacgcgg
     1861 atccgaagcg gttccactac ctgccggacg ggccgcggct gcgcttcggc ccccctgaag
     1921 gagatcagga aggcgagccg gagctgtcgc tcctcaaata ccggctggac ccggccctcc
     1981 acaccgagct gggggccggg ctcctctccc tcaccgtcga cctcggcgtg gacgaggaga
     2041 ggctcgagcg tctgcggggg cgcctggccg cccgcgccgg cgtctcgggc cccgtgctct
     2101 cccccgtcgc cgccgactcc gggagctgcg agctggtgct catcgatcgg tcgtccaccg
     2161 atgagacagc cgccacgggc ttcggcctgg tggcccgcat cctcggggcg gccaccccct
     2221 ccctctatgg cgacaacgcc gcgacgttca tggcggtgct gtcggccgag gggatggcgc
     2281 tggccgaggg ggcgctgcgc ggcggcggcc tgcccgccgg ggtggtctac accctgcgca
     2341 ccaccgggct gcggccggcg ctcagggccg agatcaccgc ccgctggcgc gacgtctacg
     2401 agttctacga gaaccgcttc cacggcggca agctcctcct ggcggtcgac atcggcccga
     2461 cgatcgagga gctggtgcag aacgagctcc tctccatccg cgtcgacgag ctggtgccgg
     2521 ccgaggagcg gtccgcggtg ctcgaccggg cgatc
//
LOCUS       DASPJI010000489         7404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_145143, whole genome shotgun sequence.
ACCESSION   DASPJI010000489 DASPJI010000000
VERSION     DASPJI010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145143"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1771)
                     /locus_tag="VEL74_13290"
     CDS             complement(167..1771)
                     /locus_tag="VEL74_13290"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYG63550.1"
                     /translation="MQHDELCYLKPNVLTEPLVCNWYAWTHLLQPVTAGMNVVDRHLA
                     IMKSFVQAPQVHAAAVKNPAMVGGPFISHPPSRAKDIKALMDRTLQEQAELIALAKAV
                     KELDDLLRNEAKGYSLEPLYPRVPEMLRGYVELVYDLNHNPSFRLLEPLLYRSQYYRT
                     DLQSFALTLSEGDKERPFVLSTPRIEEEGVLFIHRPFNHEGIDELFRMKASPRPYGHI
                     KELLGIQDGADEELFRTFFTTERPAPYEAYDGDGLRTRYFGHACILIEGRGFSVLSDP
                     VISYDYHADLPRYTYADLPDFIDYVVITHNHQDHVLLETMIQLRHKIGTVVVPRGGGG
                     SLQDPSLRLVLQQLGFKNVREIEELETIELEGGGALTGVPFLGEHSDLDIRTKMAYMV
                     KVEAGSVLLAADSCNIEPRIYEHVQKITGDADILFLGMECDGAPLSWLYGPLMTRPLD
                     RKLDHSRRLSGSNYERGIDIVTRFGCKEAYVYAMGMEPWLKYIMAKDYTPDSDPIVAS
                     DRLIEDCRNRGLVAERLFGEKEIAIS"
     gene            complement(1806..2873)
                     /locus_tag="VEL74_13295"
     CDS             complement(1806..2873)
                     /locus_tag="VEL74_13295"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HYG63551.1"
                     /translation="MTRVDATRLARIMEGALRRRGLPDEHAGFVVDGLLEASLRGVDT
                     HGVRLFPTYLAELDGGRSRARPEMRWLGSAPAARVLDAGHALGIVAGRVACDEAVRLA
                     RENGVGTVAVRNSNHFGPASCYTLAMARQGALGLALTNSDALVAPYNGRLPFFGTNPI
                     SLAAAGEGEDTFCADFATSQVSYSRVKRYREEERPLSPGWALTPDGRDAAAASPEDGG
                     TEEVGALQPLGGHKGQCLGMMVEVLSAVLAGMPFDHELSHLYAPPYDEPRRVAHLFIA
                     FHLPAFGDPDGFRQRLSRLMGLVRDQPGVGGERVHVPGDPEIETAAERRQQGIPLSAG
                     EWAWFEAVDREGERECAAPGC"
     gene            complement(2870..4195)
                     /locus_tag="VEL74_13300"
     CDS             complement(2870..4195)
                     /locus_tag="VEL74_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HYG63552.1"
                     /translation="MVEIALKGCFREPLLDVMNFLNEVVLDHPGAISFAPGRPTERYF
                     DVERSLGRVAPFVEHRAAALGWSGAAVWNDLGQYNRTNGIINGLIARQIELDEGIRAD
                     PESIVITTGCQEGMVILLLALLDPAADVLLVSDPSYIGIPGLARMMGIPMQPIPTGEH
                     GLDPARVAAAIGEVRRSGRRPRALYDVPDFNNPLGTRMPVAARRELLALARDNDLLIF
                     EDNPYGMFSYDGAPLPTLKSLDEHGAVIYMGSFSKSLYPGLRLGFLVAGQTVVAPDGS
                     RTTLAAELSKVKSLTTVTTPPLLQAIVGGILLESGGSLAPLMREKLPFYRANRDRMVA
                     SLDAELGDLAPAVRWNRPEGGFFLTVDLPFEFGEAHLRACAREHGVIVCPMSFFALTP
                     GRERQVRLSFSYVSEEQIEDGVRRFARFVREQAGAGRLEPAVASADRLP"
     gene            complement(4258..4779)
                     /locus_tag="VEL74_13305"
     CDS             complement(4258..4779)
                     /locus_tag="VEL74_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYG63553.1"
                     /translation="MSEKESPASVASLVAMHKYATSVDHVAIAVQDLEESIKFYTEVL
                     GFELRERRKTEGKTTAMVSAVIKAGPITFVLLQGTSPDSQVSKFVENFGPGVQHVAIG
                     VQNLPEMAEELKAAGMEFDTTVIQSPGLRQIFSKRDPGSGMMYEFIERLGGDFSDDSV
                     QQLFMQLEQKDSY"
     gene            4993..5379
                     /locus_tag="VEL74_13310"
     CDS             4993..5379
                     /locus_tag="VEL74_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63554.1"
                     /translation="MNTRAFGKLALAAVLLVATLAVAGVAVNRGEGAELRRVETHKVC
                     MINNQVFEKDQIPVAIEGRTYYGCCEMCKERLARDTGARTAVDPVSGKTVDKAKAVIG
                     ARPDGSVLYFESEENLAQYREPAASR"
     gene            complement(5442..5690)
                     /locus_tag="VEL74_13315"
     CDS             complement(5442..5690)
                     /locus_tag="VEL74_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63555.1"
                     /translation="MTLKKSLLALGVLALVGAAQVTTAVSAPECPKPRRFSGGCIQVI
                     TYAKNPDTGTCCEYPNPCSAPQGWATYFSLSECEAATL"
     gene            complement(5845..>7404)
                     /locus_tag="VEL74_13320"
     CDS             complement(5845..>7404)
                     /locus_tag="VEL74_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HYG63556.1"
                     /translation="RAEALDEQIAFEVVDDGPGIPEDEQERIFERFHQVDASLSREQA
                     GTGLGLALARELARLHGGEVTVRSRPGEGSAFRVELPRHLEPWPERRRRPRRREDQLA
                     QTRTEAQAAREYAWRSMRDTLLSDIEQPRLVRHVLPLPEPPPEAPRVLLVEDNDDLRS
                     FVASRLLRRYRVDTAADGAAGLETARRSPPDLVVADVMMAGMDGYELCRRLKADPLFA
                     TVPIILVTARAGSEAVAEGLEVGADDYVVKPFAMRELEARIAAHLRARETERQLHERE
                     SRLAAIGQMVSSVVHDLRNPLTLIKGYTELAHTVAQRGGNPEAIARDLDQVRSASERL
                     RHMIEEILDFARGGAPRLQLEPVPARRFLDRVLAPLCADLAERGIATDLDLGLPEDLQ
                     VSLDVDRMQRVIENLVINAREALAVWEGEKRLLVSARVEEGCLALRVADTGPGIPEEA
                     AAHLFEPFATAGKKRGTGLGLVTVRNLVKAHGGDVRAEPRAPEGGAAFRLTLPLATPE
                     KARSPQRATLV"
BASE COUNT         1124 a         2500 c         2651 g         1129 t
ORIGIN      
        1 gcagggggag cgatccgtcc gggaacagcg gctcggtctg gaccaggctc tcctgggaga
       61 cgctcaccgc gcggcgggtg atgccgccga tcttcttgag gctgggcatg ccgcccgatt
      121 caccgatact catgggttcg attctccaat cagtcgaggg gctgcctcag gaaatggcga
      181 tctctttttc gccgaacagc cgctcggcca cgaggccgcg gttgcggcag tcctcgatga
      241 gccggtcgga ggcgacgatg ggatcggagt cgggggtgta gtccttggcc atgatgtatt
      301 tgagccaggg ctccatcccc atggcgtaga cgtacgcctc tttgcagccg aaacgggtca
      361 cgatgtcgat gccgcgctcg tagttcgagc ccgagaggcg gcgcgagtgg tcgagcttgc
      421 ggtcgagcgg ccgggtcatc agcgggccgt agagccacga cagcggcgcg ccgtcgcact
      481 ccatgccgag gaagaggatg tccgcgtcgc cggtgatctt ctgcacgtgc tcgtagatcc
      541 gcggctcgat gttgcaggag tcggcggcga gcagcacgct gccggcctcc accttgacca
      601 tgtaggccat cttggtgcgg atatcgaggt cgctgtgctc gccgaggaac ggcacgccgg
      661 tcagcgcacc gccgccctcg agctcgatgg tctccagctc ctcgatctcg cgcacgttct
      721 tgaacccgag ctgctgcagc accagccgca gcgacgggtc ctgcaggctg ccgccgccgc
      781 cgcgcggcac caccacggtg ccgatcttgt ggcggagctg gatcatggtc tcgagcagca
      841 cgtggtcctg gtggttgtgg gtgatcacca cgtagtcgat gaagtccggc aggtccgcgt
      901 aggtgtagcg cggcaggtcg gcgtggtagt cgtagctgat cacgggatcg gagaggacgc
      961 tgaagccgcg cccctcgatg aggatgcagg cgtggccgaa gtagcgggtg cgcaggccat
     1021 cgccgtcgta ggcctcgtag ggcgcgggcc gctcggtggt gaagaaggtg cggaacagct
     1081 cctcgtccgc cccgtcctgg atgccgagca actccttgat gtggccgtag gggcgcggcg
     1141 aggccttcat gcggaacagc tcgtcgatcc cttcgtggtt gaacggccgg tggatgaaga
     1201 ggaccccctc ctcctcgatg cgcggcgtgc tcagcacgaa cgggcgctcc ttgtccccct
     1261 cgctcagggt cagcgcgaag ctctggaggt ccgtccggta gtactggctg cggtagagga
     1321 gcggctcgag cagccggaac gacgggttgt ggttgaggtc gtagaccagc tccacgtagc
     1381 cgcggagcat ctccggcacg cgcggataga gcggctccag ggagtagccc ttggcctcgt
     1441 tgcgcagcag gtcgtccagc tccttgaccg ccttggcgag ggcgatcagc tcggcctgct
     1501 cctgcagcgt ccggtccatg agcgccttga tgtccttggc gcgggacggc ggatggctga
     1561 tgaaggggcc gcccaccatc gcagggttct tgaccgccgc cgcgtgcacc tgcggcgcct
     1621 ggacgaagga cttcatgatc gccaggtggc ggtcgacaac gttcatgccc gcggtgaccg
     1681 gctggagcag atgggtccac gcgtaccagt tgcagaccag cggctcggtc aggacgttgg
     1741 gcttgaggta gcagagctcg tcgtgttgca tgatgggttc ctcctaatgc tggggccttg
     1801 gaattctagc atcccggtgc ggcgcactcc cgctcccctt cccgatcgac ggcttcgaac
     1861 caggcccact ctccggcaga cagggggatc ccctgctgcc gccgttcggc cgccgtctcg
     1921 atctccgggt ctcccggcac atggacgcgc tcgcccccca cccccggctg gtcgcgcacc
     1981 aggcccatga gccgggagag gcgctggcgg aacccgtccg ggtcgccgaa cgccggcagg
     2041 tggaaggcga tgaagagatg ggcgacgcgg cgcggctcgt catagggggg ggcgtagagg
     2101 tgcgacagct cgtggtcgaa cggcatcccg gccagcacgg cgctcaggac ctcgaccatc
     2161 atacccaggc actggccctt gtggccgccc aggggttgca gcgcgccgac ctcctcggta
     2221 ccgccgtcct cagggctggc cgccgcggcg tcccgaccgt cgggggtgag ggcccagccc
     2281 ggagacaggg gccgctcctc ttcgcggtag cgcttgaccc gcgagtagga gacctggctg
     2341 gtggcgaagt cggcgcagaa cgtgtcctcc ccctccccgg cggcggcgag cgagatcggg
     2401 ttggtgccga agaacggcag ccggccgttg tacggcgcca ccagcgcgtc gctgttggtc
     2461 agcgccagcc cgagcgcccc ctggcgcgcc atcgccaagg tgtagcagga ggccgggccg
     2521 aagtggttcg agttgcgcac cgccacggtg ccgacgccgt tctcgcgggc gaggcgcacc
     2581 gcctcgtcgc aggcgacccg acccgccacg atgcccaggg cgtggccggc gtcgagcacc
     2641 cgcgccgccg gggcggagcc gagccagcgc atctcgggac gggcgcggct gcggccgccg
     2701 tccagctcgg ccaggtaggt ggggaacagc cgcacgccgt gggtgtcgac gccgcgcagc
     2761 gacgcctcca gcagaccgtc gaccacgaaa ccggcgtgct cgtcggggag cccgcgccgg
     2821 cgcagggccc cctccatgat gcgggcgaga cgggtggcgt cgacgcgcgt cacggcaggc
     2881 ggtccgcgct ggcgactgcg ggctcgagcc gcccggcgcc ggcctgctcc cgcacgaagc
     2941 gggcgaagcg gcggacgccg tcctcgatct gctcctcgga gacgtagctg aacgagaggc
     3001 gcacctgccg ctcccgtccg ggggtcaggg cgaagaagga catcgggcag acgatgacgc
     3061 cgtgctcccg cgcgcaggcc cggaggtgcg cctcgccgaa ctcgaagggg aggtcgacgg
     3121 tgaggaagaa gcccccctcc ggccggttcc agcgcaccgc cggcgccagg tcccccagct
     3181 ccgcgtcgag ggaggccacc atgcggtcgc ggttggcgcg atagaacggc agcttctccc
     3241 gcatcagcgg cgccagcgat cctccgctct ccagcaggat gccgccgacg atcgcctgga
     3301 gcagcggcgg cgtcgtcacc gtggtcaggc tcttgacctt ggacagctcg gccgccagcg
     3361 tcgtgcgcga gccgtcgggc gccaccaccg tctggccggc gacgaggaag ccgaggcgca
     3421 ggccggggta gaggctcttc gaaaagctcc ccatgtagat cacggccccg tgctcgtcga
     3481 gcgacttgag cgtcggcagc ggcgcgccgt cgtaggagaa catgccgtag gggttgtcct
     3541 cgaagatcag caggtcgttg tcgcgcgcca gcgccagcag ctcgcggcgg gccgccaccg
     3601 gcatccgggt gcccagcggg ttgttgaagt ccggcacgtc gtagagggcg cgggggcgcc
     3661 gccccgagcg ccgcacctcg ccgatggcgg cggcgacccg ggcggggtcg aggccgtgct
     3721 cgccggtggg gatcggctgc atcgggatcc ccatcatgcg ggcgaggccg gggatgccga
     3781 tgtaggacgg atcgctcacc agcagcacgt cggccgccgg gtcgagcagg gccaggagga
     3841 ggatgaccat cccctcctgg cagccggtgg tgatgacgat cgactcggga tcggcgcgga
     3901 tcccctcgtc gagctcgatc tgccgggcga tcaggccgtt gatgatgccg ttggtccggt
     3961 tgtattggcc gaggtcgttc cagaccgcgg cgccggacca acccagggcg gcggcgcgat
     4021 gctcgacgaa cggcgccacc cggccgaggc tccgctccac gtcgaagtag cgctcggtgg
     4081 gccggcccgg agcgaacgag atcgccccgg ggtggtccag caccacctcg ttgaggaagt
     4141 tcatcacgtc gagcagcggc tcgcggaagc agcccttgag ggcgatctcc accatctccg
     4201 gtctctctcc agtctctccg gccttggtct ttccggccaa cctctccggg ccgcggctca
     4261 gtaggagtcc ttctgctcca actgcatgaa aagctgctgc acgctgtcgt cggagaagtc
     4321 gccgcccagc cgctcgatga actcgtacat catgccggag ccgggatcgc gcttgctgaa
     4381 gatctgccgc aggccggggc tctggatcac cgtggtgtcg aactccatgc ccgccgcctt
     4441 gagctcctcc gccatctcgg gcaggttctg gacgccgatg gcgacgtgct ggacgccggg
     4501 cccgaagttc tcgacgaact tcgacacctg cgagtccggc gaggtcccct ggagcagcac
     4561 gaaggtgatc gggccggcct tgatcaccgc cgagaccatg gccgtcgtct tgccttcggt
     4621 cttgcgccgc tcgcgcagct cgaagcccag aacctcggtg tagaacttga tcgattcctc
     4681 gaggtcctgg acggcaatgg cgacgtgatc gaccgaggtg gcgtacttgt gcatcgccac
     4741 cagggaggcc acggaagcgg ggctttcttt ttcgctcatg gacggctctc ctatcgttta
     4801 gaaattgatg cttatgatcg agggctgctg agaccgagcg cgggtgtaca gcgcctacaa
     4861 ctgtctagtg ctccgaaaac gaaaaccgag acgactcccc ctcagatcgg ggcggccggc
     4921 acagggttgt cacgcaataa cccacgggcc cgccgacgtt ctgtctctgg agacgaacga
     4981 ggagatccac ccatgaatac gagagctttc ggcaagttgg ccctggcggc cgtcctcctg
     5041 gtggcgactc tggcggtggc cggcgtggcg gtgaaccgcg gcgagggcgc cgagttgcgc
     5101 cgcgtcgaga cccacaaggt ctgcatgatc aacaaccagg tgttcgagaa ggaccagatc
     5161 ccggtggcca tcgagggccg cacctactac ggctgctgcg agatgtgcaa ggagcgcctc
     5221 gcccgcgaca ccggcgcccg caccgccgtc gatccggtga gcggcaagac cgtggacaag
     5281 gccaaggccg tcatcggcgc ccgccccgac ggcagcgtcc tctacttcga gagcgaggag
     5341 aacctcgcgc agtaccgcga gcccgcggcc tcccgctgag gcagaacggg ccggcgcccc
     5401 gaagagtcgc cggcccagag agaggcaggg gtgctggagc gctacaaggt cgccgcctcg
     5461 cactccgaca gcgagaagta ggtggcccag ccctgggggg cggagcaggg gttcggatat
     5521 tcgcagcagg tgccggtgtc agggttcttg gcgtaggtga tcacctggat gcagccaccg
     5581 ctgaaacggc gcggcttcgg gcactccgga gccgagacgg cggtggtgac ctgagcggca
     5641 ccgacgagag ctaaaacacc aagggcgagc agagacttct tcagagtcat aacttctcct
     5701 tcattgagtc atccatcctt ttcgggtggg gaccccaacc ctgtcgcgct cgagccgaag
     5761 taatgatcac tcaccgctcg tagaaagtca agtcgatacc acggaaactg tatatctctc
     5821 agattaacaa ccataacctc ccagctatac aagtgtcgcc cgctgcgggc tccgggcctt
     5881 ctccggcgtc gccagaggca acgtgagccg gaacgcggcg cccccctccg gcgctctagg
     5941 ctcggcgcgg acgtcgccgc cgtgcgcctt gaccaggttg cgcacggtga cgagccccag
     6001 gccggtgccc cgcttcttgc ccgcggtggc gaagggctcg aagaggtggg cggccgcctc
     6061 ctccgggatg cccgggccgg tgtcggcgac ccggagggcc aggcacccct cctccacccg
     6121 ggcgctcacc aggagccgct tctccccctc ccagaccgcc agggcctcgc gggcgttgat
     6181 caccaggttc tcgatcaccc gctgcatacg atccacgtcg agagagacct ggaggtcctc
     6241 gggcaggccg aggtcgagat cggtggcgat cccgcgctcg gcgaggtcgg cgcagagcgg
     6301 cgcgaggacc cggtcgagga agcggcgggc cggcaccggc tcgagttgga gccgcggcgc
     6361 gccgccgcgg gcgaagtcga ggatctcctc gatcatgtgg cgcagccgct cggaggcgct
     6421 ccgcacctga tccaggtcgc gggcgatggc ctcggggttg ccgccgcgct gggcgacggt
     6481 gtgagccagc tcggtatatc ccttgatcag cgtcaggggg ttccgcaggt catgaaccac
     6541 ggagctgacc atctggccga tggcggcgag acggctctcc cgctcgtgga gctggcgctc
     6601 ggtctcgcgg gcgcgcaggt gggcggcgat gcgggcctcc agctcgcgca tggcgaaggg
     6661 cttgaccaca tagtcgtcgg cccccacctc cagcccttcg gccaccgcct cggagccggc
     6721 ccgggcggtg accaggatga tggggacggt ggcgaagagc ggatcggcct tgaggcgccg
     6781 gcacagctcg tagccgtcca tgcccgccat catgacgtcg gcgaccacca ggtccggcgg
     6841 cgagcggcgg gcggtctcca acccggcggc gccgtcggcg gcggtgtcga cgcgatagcg
     6901 gcgcagcagg cggctggcga cgaagctccg caggtcgtcg ttgtcctcca ccaggaggac
     6961 gcgcggcgcc tccggcggcg gctcgggcag ggggaggacg tgccgcacga ggcgcggctg
     7021 ctcgatgtcg gagaggaggg tgtcgcgcat ggagcgccag gcgtactcgc gcgccgcctg
     7081 ggcctcggtg cgggtctgcg cgagctggtc ctcgcgccgg cgcggccgcc ggcggcgctc
     7141 gggccacggc tcgaggtggc gcggcagctc gacgcggaag gccgagccct cgccggggcg
     7201 gctccgcacc gtcacctccc cgccgtgaag ccgggccagc tcccgggcca gggcgaggcc
     7261 gagccccgtc cccgcctgct cgcgcgagag ggaggcgtcc acctggtgga agcgctcgaa
     7321 gatcctctcc tgctcgtcct cggggatccc ggggccgtcg tccaccacct cgaaggcgat
     7381 ctgctcgtcc agcgcctcgg cccg
//
LOCUS       DASPJI010000490         8060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_376408, whole genome shotgun sequence.
ACCESSION   DASPJI010000490 DASPJI010000000
VERSION     DASPJI010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8060)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8060
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376408"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VEL74_13325"
     CDS             complement(<1..813)
                     /locus_tag="VEL74_13325"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HYG63557.1"
                     /translation="MRRSTVFVAGLIALVLALAAAPVTRAQVAGLYYAEVEKDGRIYV
                     FNTPEKKKLFEETGDMGVGITMPGAGADGVTLVAENETAADLYFFKHDLPGYDRPTPK
                     PASPAFTIGWKDGKTSIETKSALVQISNRVQLRFTEAMPEVGEDRGSFRIRRAKTKFE
                     GWVYNKNLTYELQLNWPDTANPLEDAVINYDFTQGKKAFQLKAGQFKVPFGRQELTSS
                     GSQEFVDRSIVSNFFARGRDIGLQAWGTPAGGKIDWRVGLFNGNGRTVSANDN"
     gene            1145..2077
                     /locus_tag="VEL74_13330"
     CDS             1145..2077
                     /locus_tag="VEL74_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HYG63558.1"
                     /translation="MALTGSLLAAQGARVDSGLATYQKTSGVSGNFNSVGSDTLNNLM
                     TLWAEGFRKQYPNVRIQIEGKGSSTAPPALISGTAQLGPMSRVMKSTEIDDFEKKYGY
                     PPTPIRVAVDALAVYVNKDNPLDKLTLQQVDAVFSKGRTCGAKKAVGNWGSVGLTGDW
                     AGRPMSLYGRNSASGTYGYFKEHALCKGDFKDTVKEQPGSASVVQGVTEDKFAIGYSG
                     IGYKTSGVKALSLADKSGQFFEATPENALSGKYPLSRFLYVYVNKAPNKPLDPLVREF
                     IKYALSQEGQQNVVKDGYVPIPARIAKEELAKLQ"
     gene            2165..4432
                     /locus_tag="VEL74_13335"
     CDS             2165..4432
                     /locus_tag="VEL74_13335"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYG63559.1"
                     /translation="MSPSSSSRLGSGETPRRRLLADRLARWIVSAGGLAIIFSVLGIL
                     VFILLEVWPLTTGARVGLSQPVPLAGPRAGALLADEHRTHVAALGEDGLVRVVRTRDG
                     AVTTTLDLLAQAQQPGQPPGEAPVREPQLLTDMDVPAGSQALAAATRDGRVLIAPIRF
                     EITFEGQNRVVAPAIDTPVSLVLDPAGRPLRAFAAQLGDDGGATAAAVLADGSLAIVR
                     RTAEENLMTGEVTESLERFQSRVPGAGEVADLVMDRDRRNLYGGTTDGKVLWWSLEGE
                     PGDPQVTSAGPSPVTAAGLLIGGRSLVVGQEDGSVSVWFQVRQPDETYRLTRVRDFPA
                     LTGPVRAIAASQRDRSFLVYAANGRMGLYHSTAHRTLWTGEVPVEGGTSLAYTPKADG
                     AYLAGGGKLEEIRIHNPHPEVTWKSLFGKTWYEGADKPEYVWQSSSGTDDFEPKLSLT
                     PLLAGTLKGTFYSLLLAIPLGVFGAMYASQFMHPAYKRYLKPLIEIMASLPSVVLGFL
                     AGLWLAPRVEQAMPGLILMLIVLPLFVLAAGFLWRSLPRTFRGRFRVGTEAILFIFVL
                     AFGIWVCFQLSEPFERLVFGGNFQGWLLDATGLNYDQRNAVVVGLAMGFAVIPIIFAI
                     AEDAFSNVPPNLVAGSLALGADRWQTVTRVVLPTASPGIFSAIMVGFGRAVGETMIVL
                     MATGNTPIMEWNPFNGFRTLSANIAVEIPEAPQGGTLYRTLFLAALLLFIATFIINTV
                     AELVRQRLRERYSQL"
     gene            4456..6210
                     /gene="pstA"
                     /locus_tag="VEL74_13340"
     CDS             4456..6210
                     /gene="pstA"
                     /locus_tag="VEL74_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HYG63560.1"
                     /translation="MNPSVSQASAESVVDRARPAALSTRRTREKATGATGFFGDHRRR
                     SELVGQSLTWLCGGALAFNVLLVLGIMILLAWNGLGYFWQRDLVEIAMKDGTKVLGEV
                     WEIEQVPAEPGQAADTAIDRVRVKTANRDIGGLDFAWIDESAIAGRRLPAQAVLLERQ
                     EWGNFHGFMTELREGGKVVARGPEPVWERFEILHERAMERRERVEELEKGAIGDVNYE
                     IEQLRLDRRKLEIQELPAAELQRRAAEIDRRLEERQAEYQQLADRLFKMREEMLADTL
                     VMQTVAGETQEMPVGNVVRAVRPNDMGWLDKAGLYFSRGWEFIAADPRESNTEGGIFP
                     AIFGTVMMVFIMSFAVAPLGVLAALYLREYAKQGLVVRLVRIAVNNLAGVPSIVFGVF
                     GLGFFVYLIGGSLDQLLYPEALPTPTFGTGGILWASLTLALLTVPVVIVAAEEGLAAV
                     PRIVREGSLALGATKFETIRRVVIPAAAPGILTGMILAMARAAGEVAPLMIVGMVKLA
                     PTLPIDARPPFVHLERKFMHLGFHIFDVGFQSPNIEATTPLVFATALLLIVVVTVMNL
                     VAISLRNHLRRKYAGSAI"
     gene            6328..7071
                     /gene="pstB"
                     /locus_tag="VEL74_13345"
                     /pseudo
     CDS             6328..7071
                     /gene="pstB"
                     /locus_tag="VEL74_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019584515.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
     gene            7120..>8060
                     /locus_tag="VEL74_13350"
     CDS             7120..>8060
                     /locus_tag="VEL74_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63561.1"
                     /translation="MSRTFLVDDARVRSLSATRQGRVSLGGFAYQAGYAVARLASLHA
                     RRPVLDLPGVPVLLRYDWAEDLDEMETDGRAVFTQCKRIADIGQPASLAGILTGFAPK
                     WLWAPEERRTELRFRLVCPDLRFAGRGPVPLAADRRRAEVLAAAESLLAKPPGRSSDR
                     ALWQTEAEAAGFGPLLEALWDQTEVLHLPAEPLTDDPAGPLLPAERSALELLVRCQAV
                     VPSRQRQALAELRSLLHSNVVELDLTGDRPVPVLDRAPRVLDGDDVRYALFGIQMPSE
                     EQPPFRVVTRLFLEEEAVKPRRPYVARPPQWADVVHG"
BASE COUNT         1219 a         2889 c         2674 g         1278 t
ORIGIN      
        1 gttgtcgttg gcgctcaccg tgcggccgtt gccgttgaaa agaccgacgc gccagtcgat
       61 cttgccgccg gccggggtgc cccaggcctg caggccgatg tcgcggccgc gggcaaagaa
      121 gttggagacg atggagcggt cgacgaactc ctgggagccc gaggaggtca gctcctggcg
      181 gccgaagggg accttgaact ggccggcctt gagctggaac gccttcttgc cctgggtgaa
      241 gtcgtagttg atgacggcat cctcgagcgg gttggcggtg tcgggccagt tgagctgcag
      301 ctcgtaggtg aggttcttgt tgtagaccca gccctcaaac ttggtcttgg cgcggcggat
      361 gcggaacgag ccccggtcct cccccacctc gggcatcgcc tcggtgaagc ggagctgcac
      421 ccggttcgag atctgaacca gggcgctctt ggtctcgatg ctggtcttgc cgtccttcca
      481 gccgatggtg aacgccgggc tcgccggctt cggcgtcggc cggtcgtagc cggggaggtc
      541 gtgcttgaag aagtagaggt cggcggcggt ctcgttctcg gccacgagcg tcacgccgtc
      601 ggcgccggcg cccggcatgg tgatgcccac gcccatgtct cccgtctcct cgaagagttt
      661 cttcttctcg ggcgtgttga agacatagat gcggccgtcc ttctccacct ccgcgtaata
      721 gaggcccgcc acctgcgcgc gggtgacggg ggcggccgcc agcgccagga cgagcgcgat
      781 gaggccggcc acaaacacag ttgatctgcg cattccaact ctccttttat gggtcacggt
      841 ttcgaagctc cgaagctgaa tcgagggtga ggataggaag ccgcgccaac gaccctgcga
      901 cgcgccggtg acaggcctgc gacagccggc cggtggcgcg ccggcccgcg aattctgtca
      961 ccccgccgtc acacgatggt aggtccgctg ccaaacccct ctgccagagt catgctgccg
     1021 gacgggtcgc tcatcacccg atatcacccg ataccggccc cgatccaagc acgttcatcg
     1081 aatagagaga taaggagaga gaacgatgag gcatgacttc aaggtggctt ccttcgccgt
     1141 ggcgatggcc ctgacgggga gcctcctggc cgcccagggc gcgcgggtgg actccggcct
     1201 ggcgacctat cagaagacca gcggtgtctc ggggaacttc aactcggtgg gctcggacac
     1261 cctcaacaac ctgatgacgc tgtgggccga gggcttccgc aagcagtacc cgaacgtgcg
     1321 gatccagatc gagggcaagg gctcctccac cgctcccccg gcgctcatct ccggcaccgc
     1381 gcagctcggc cccatgagcc gcgtcatgaa gtcgaccgag atcgacgact tcgagaagaa
     1441 gtacggctac ccccccaccc cgatccgggt ggcggtcgac gccctggcgg tctacgtcaa
     1501 caaggacaac ccgctcgaca agctgaccct gcagcaggtg gacgcggtct tctccaaggg
     1561 ccgtacctgc ggcgccaaga aggcggtcgg caattggggc agcgtcggcc tcaccggcga
     1621 ctgggccggc aggccgatga gcctctacgg ccgcaactcc gcctccggca cctacggtta
     1681 cttcaaggag cacgcgctct gcaagggcga cttcaaggac accgtcaagg agcagcccgg
     1741 ctccgcctcg gtggtccagg gcgtgaccga ggacaagttc gccatcggct acagcggcat
     1801 cggctacaag acctcgggcg tcaaggcgct gtcgctggcc gacaagagcg gccagttctt
     1861 cgaggcgacg ccggagaacg ccctgtccgg caagtacccg ctgtcgcgct tcctctatgt
     1921 ctacgtcaac aaggcgccca acaagccgct cgacccgctg gtccgggagt tcatcaaata
     1981 cgccctgtcg caggagggcc agcagaacgt ggtgaaggac ggctacgtcc ccatcccggc
     2041 ccgaatcgcc aaggaagagc tggccaagct ccagtaggtc cgtggcctcg ccgcggcccg
     2101 gtggtccgat cacggccgcc gggccgcttt tccatagatt ctctagaccg gaacccagaa
     2161 cctcgtgagc ccatcctcct cctctcgatt gggaagcggc gagaccccca ggcggcggct
     2221 cctcgccgat cgcctggccc gctggatcgt gtccgcgggc ggtctcgcca tcatcttcag
     2281 cgttctcggc atcctggtgt tcatcctgct ggaggtctgg ccgctgacga ccggagcgcg
     2341 ggtgggcctg tcgcagccgg tccccctggc gggcccccgt gccggcgccc tgctcgccga
     2401 cgagcaccgg acgcacgtcg cggccctcgg ggaagacggc ctggtgcgcg tcgtgcgcac
     2461 ccgcgacggc gcggtcacca caacgctcga cctgctcgcc caggcgcagc agcccggcca
     2521 gccgcccggc gaggccccgg tccgggagcc ccagctcctc accgacatgg acgtccccgc
     2581 cggcagccag gccctggccg ccgcgacccg cgacggccgg gtgctcatcg cccccatccg
     2641 cttcgagatc accttcgagg ggcagaaccg ggtggtcgcg ccggcgatcg atacgccggt
     2701 gtcgctggtc ctcgaccccg cgggccggcc gctgcgggcc ttcgccgccc agctcgggga
     2761 cgacggcggc gccactgccg cggcggtcct cgccgacggc tccctggcca tcgtgcgccg
     2821 caccgccgag gagaacctga tgaccggcga ggtcacggag tcgctcgagc gcttccagtc
     2881 tcgggtcccc ggcgccgggg aggtggccga cctggtgatg gaccgcgacc gccgcaacct
     2941 ctacggcggc accaccgacg gcaaggtcct ctggtggagc ctcgaaggcg agcccggtga
     3001 cccgcaggtg acctccgccg gcccctcccc cgtcaccgcc gccggcctgc tgatcggcgg
     3061 ccgctcgctg gtggtggggc aggaggacgg ctcggtcagc gtctggttcc aggtgcgcca
     3121 gccggacgag acctaccggc tgacccgcgt gcgcgacttc ccggccctga ccggaccggt
     3181 gcgcgccatc gctgcctcgc agcgggaccg gagcttcctc gtctatgccg ccaacggccg
     3241 gatggggctc taccactcca ccgcccaccg cacgctgtgg accggcgagg tgccggtcga
     3301 gggaggcacg agcctcgcct ataccccgaa ggccgacggc gcctacctgg ccggcggtgg
     3361 caagctcgag gagatccgga tccacaaccc ccaccccgag gtcacctgga agtccctgtt
     3421 cggcaagacc tggtacgagg gggccgacaa gcccgagtac gtctggcagt cgagcagcgg
     3481 caccgacgac ttcgagccca agctgtcgct caccccgctg ctcgccggca ccctcaaggg
     3541 gaccttctac tcgctgctcc tcgcgatccc gctgggcgtc ttcggcgcca tgtacgcgtc
     3601 gcagttcatg cacccggcct acaagcgtta cctgaagccg ctgatcgaga tcatggcgtc
     3661 gctgcccagc gtcgtgctcg gcttcctcgc cggcctgtgg ctcgcgccgc gcgtcgagca
     3721 ggccatgccg gggctgatcc tgatgctcat cgtgctgccg ctcttcgtgc tggccgccgg
     3781 cttcctctgg cggtcgctgc ccaggacgtt ccgcggccgg ttccgcgtcg gcaccgaggc
     3841 gatcctcttc atcttcgtgc tggcgttcgg gatctgggtc tgcttccagc tctcggagcc
     3901 gttcgagcgg ctggtcttcg gcggcaactt ccagggctgg ctgctcgacg ccaccggcct
     3961 caactacgac cagcgcaacg ccgtggtggt gggcctggcg atgggcttcg cggtcatccc
     4021 gatcatcttc gccatcgccg aggacgcctt ctcgaacgtg ccgcccaacc tggtcgccgg
     4081 ctcgctggcc ctgggcgccg accgctggca gacggtcacc cgcgtggtgc tgccgaccgc
     4141 ctcgccgggc atcttctccg ccatcatggt cggcttcggg cgcgccgtgg gcgagaccat
     4201 gatcgtgctc atggcgaccg gcaacacgcc gatcatggag tggaacccgt tcaacggctt
     4261 ccgcaccctg tcggccaaca tcgccgtcga gatccccgaa gcgccgcagg gcggcaccct
     4321 gtaccgcacc ctgttcctgg cggcgctcct gctgttcatc gcgacgttca tcatcaacac
     4381 ggtggcggag ctcgtgcgcc agcggctgcg cgagcggtac tcacagctct gatcatggct
     4441 cgggagaaga ccgatatgaa tccctccgtt tcccaggcct cggccgagag cgtggtggac
     4501 cgcgccaggc cggccgccct atcgacgcgc cggacgcgcg agaaggccac cggcgccacc
     4561 ggcttcttcg gtgaccaccg gcgccgctcg gagctcgtcg gccagtccct cacctggctg
     4621 tgcggcggcg cgctggcgtt caacgtcctg ctggtcctcg gcatcatgat cctgctggcc
     4681 tggaacggcc tcggctactt ctggcaacgc gatctggtgg agatcgccat gaaggacggc
     4741 accaaggttc tgggcgaggt ctgggagatc gagcaggtcc ccgccgagcc gggccaggcc
     4801 gccgacacgg ccatcgaccg cgtccgcgtc aagacggcca accgcgacat cggcggcctc
     4861 gacttcgcct ggatcgacga gagcgccatc gccggccgcc ggctccccgc ccaggcggtg
     4921 ctcctggagc gtcaggagtg gggcaacttc cacggcttca tgaccgagct ccgcgagggg
     4981 ggcaaggtcg tggcccgcgg cccggagccg gtgtgggaga ggttcgagat cctccacgag
     5041 cgggcgatgg aacgccggga gcgtgtcgag gagctcgaga agggggccat cggcgacgtc
     5101 aactacgaga tcgagcagct ccgcctcgac cgccgcaagc tggagatcca ggagctcccc
     5161 gccgccgagc tccagcgccg cgccgccgag atagaccgcc ggctcgagga gcgccaggcc
     5221 gagtaccagc agctcgcgga ccgcctgttc aagatgcgcg aggagatgct cgccgacacc
     5281 ctggtcatgc agacggtggc cggcgagacc caggagatgc cggtgggcaa cgtcgtccgc
     5341 gccgtccgcc ccaacgacat gggatggctg gacaaggcgg gcctctactt ctcccgtggc
     5401 tgggagttca tcgccgccga cccgcgggag tcgaacaccg aggggggcat cttccccgcc
     5461 atcttcggca cggtgatgat ggtcttcatc atgtcgttcg ccgtcgcgcc gctcggggtg
     5521 ctggcggcgc tctacctgcg ggagtacgcg aagcagggac tggtggtgcg cctggtgcgg
     5581 atcgcggtca acaacctggc cggcgtgccg tcgatcgtct tcggcgtctt cggcctgggc
     5641 ttcttcgtgt atctgatcgg cggctcgctc gaccagctcc tctaccccga ggcgctgccg
     5701 acgcccacct tcggcaccgg cggcatcctc tgggcgagcc tgacgctggc gctgctcacc
     5761 gtgccggtgg tcatcgtcgc cgccgaggag gggctggcgg cggtgccgcg catcgtgcgc
     5821 gaggggtcgc tcgccctggg cgccacgaaa ttcgagacta tccggcgggt ggtcatcccg
     5881 gccgccgccc cgggcatcct caccgggatg atcctggcga tggcccgcgc cgccggcgag
     5941 gtggcgccgc tgatgatcgt cggcatggtc aagctggcgc ccacgctgcc gatcgacgcc
     6001 cggccgccgt tcgtccacct cgaacgcaaa ttcatgcacc tgggtttcca cattttcgat
     6061 gtcggcttcc agagccccaa catcgaggcc accacgccgc tggtgttcgc caccgcgctg
     6121 ctgctgatcg tggtggtgac ggtgatgaac ctggtcgcca tctcgctgcg caaccatctg
     6181 cgacgcaagt acgcggggag cgccatctga ccgcagccgt tccggctgga gacgaggacg
     6241 atgctcgata ttcaaaccgc cccgacgcac ccccagacct cgcccccgcg cgatccggcg
     6301 ctcgatcccg gcatcgcgaa cccggtcctg tcgatcgagt cgctctccct ctggtatggg
     6361 gagaagcagg ccttgaagga catctcgctc aaggtgccgg agaagcagat caccgccttc
     6421 atcgggcctt cgggctgcgg caagtccacc ctgctgcgct gcatcaaccg gctcaacgac
     6481 ctgatcgacg gcgtgcgcac cgaaggggat atccgcttca acgagcgcag catcttcgac
     6541 ccgcgcctgg aaatcaacgc gctgcgcaag cggatcggca tggtgttcca gaagtcgaat
     6601 ccgttcccga aatcgatcta tgagaacgtc gcctacggtc cgcgcatcca gggagtcaag
     6661 aagaaagcgg acctggacgt gatcgtggaa aagagcctgc gcggcgccgc gctgtgggac
     6721 gaagtcaagg accggctgga ggacagcgcc ctcggcatgt cgggcggcca gcagcagcgc
     6781 ctgtgcatcg cccgggcgat cgccgtcgag cccgaggtga tcctgctcga cgagccctgc
     6841 tccgccctcg acccgatcgc cacggcgcgc atcgaggagc tgatgcagga cctgaagtcc
     6901 cagtacacgc aggtgatcgt cacccacaac atgcagcagg cttcgcgcgt ctcggactac
     6961 accgccttcc tctatctggg cgagctcatc gagtacgcga gcaccgagaa gatcttcatc
     7021 aatccgtcca agaagcagac cgaggactac atcacgggcc ggttcgggta agcggatcgg
     7081 ctaaaatggc ggcttccgta ccatgggagc cgccgtagct tgagccgtac cttcctcgtc
     7141 gacgacgcgc gcgtccgcag tctgtccgcc acccgccagg gtcgggtgag cctcgggggc
     7201 ttcgcctatc aggcgggcta cgccgtggcg cgcctggcga gcctccatgc ccggcggccg
     7261 gtgctcgacc ttcccggcgt cccggtgctg ctgcgctacg actgggccga ggacctcgac
     7321 gagatggaga cggacggccg cgccgtcttc acccagtgca agcggatcgc cgacatcggc
     7381 cagccggcca gcctcgccgg aatcctcacc ggcttcgccc cgaaatggct gtgggctccc
     7441 gaggagcggc ggacggagct gcgcttccgg ctcgtctgcc cggacctgcg cttcgccggc
     7501 cgcggtcccg tccccctggc cgccgaccgg cgccgggccg aggtgctcgc cgccgccgag
     7561 tccctgctcg ccaagccacc cggccgctcg tcggaccggg ccctctggca gacggaggcc
     7621 gaggccgccg gcttcggtcc gcttctggag gcgctgtggg atcagacgga ggtcctgcac
     7681 ctcccggccg agcccctgac cgacgacccg gccgggccgc tgctgccagc cgagcgctcc
     7741 gccctggagc ttctggtgcg ctgccaggcg gtggtgccgt cgcggcagcg ccaggcgctg
     7801 gccgagctcc gttccctcct tcactccaac gtggtcgagc tcgacctcac cggcgaccgg
     7861 ccggtccccg tcctcgaccg cgccccccgg gtgctggacg gcgacgacgt ccgctacgcc
     7921 ctcttcggta tccagatgcc ctcggaggag cagccgccgt tccgggtggt gaccaggctt
     7981 ttcctcgagg aggaggcggt caaaccccgg cgcccttacg tggcccggcc tccacaatgg
     8041 gccgacgtag tccacggcca
//
LOCUS       DASPJI010000491         6712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_89865, whole genome shotgun sequence.
ACCESSION   DASPJI010000491 DASPJI010000000
VERSION     DASPJI010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89865"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VEL74_13355"
     CDS             <1..832
                     /locus_tag="VEL74_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HYG63562.1"
                     /translation="DVYSPLVAADVPFITTDVETAELIKYASNGFLATKISFINEVAQ
                     ICEAWGANVEVVAKGMGLDSRIGPKFLNAGPGYGGSCFPKDTRAVAQIARDAGSRFRI
                     IEAVLEVNDAVQKRMITKITDAMDGVEGRTVAVLGLSFKPNTDDIRESPALPIVQGLL
                     NGGAAVRAFDPEAMPYCRPLFPAVTFCDNAYEAAEGADAVVIVTEWNQFRKLELDRLH
                     RLLQRPLVVDLRNLYEPDKMAAAGFEYVSIGRPEGRPAAQELRPVPAPMTAGAGRARA
                     "
     gene            832..1800
                     /locus_tag="VEL74_13360"
     CDS             832..1800
                     /locus_tag="VEL74_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HYG63563.1"
                     /translation="MRAVITGGAGFLGSHLCERLLGEGYEVVCVDNLVTGRLENIAHL
                     QDRPDFTFLRHDVSKPFEVEGDVDCVLHFASPASPIDYLELPIQTLKVGSLGTHNTLG
                     LAKHKRARYLLASTSEVYGDPKEHPQKESYWGNVNPVGPRGVYDEAKRFAEAMAMAYH
                     RAHGLEVRIVRIFNTYGPRMRLRDGRVVPAFIPQALTNEPMTVFGEGKQTRSFCYVDD
                     LVEGIWRLLNSDLEEPCNIGNPHEMTILEFAETIRDACGSASPIVFKPLPVDDPQTRQ
                     PDITLARTRLGWEPKVPLRQGLESTIAYFREALARNRSVESVHAES"
     gene            1787..2053
                     /locus_tag="VEL74_13365"
     CDS             1787..2053
                     /locus_tag="VEL74_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63564.1"
                     /translation="MQNHRKSLAYALEKYPERVEMVPLGELVTDSARQNPRRPAYVTL
                     QVPDDVVKSLRGREESKDLVLLVRVPKEILERSESLIVLPGEVR"
     gene            2053..2346
                     /locus_tag="VEL74_13370"
     CDS             2053..2346
                     /locus_tag="VEL74_13370"
                     /inference="COORDINATES: protein motif:HMM:NF016265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HYG63565.1"
                     /translation="MSEGTVHCARCGRTDAPALSRRPLPGAAGDEIRQRVCADCWAEW
                     LRMEVMVINELRLNFMDPAAQETLNQHMRQFLFPAEGGGEAGGGQLPGLNIQR"
     gene            2419..3114
                     /locus_tag="VEL74_13375"
     CDS             2419..3114
                     /locus_tag="VEL74_13375"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HYG63566.1"
                     /translation="MLRFLLLVLIVAAGFAALRWSPLADYLTQERLTQTFDRVRDIWW
                     SPLVLLAAYLLLCPIGVPATPLMFTGGMVYGTLYGSIYNTLGIFLGGASTFLLGRGLG
                     REFVLHLAGKRMKRVERAISRHAGFWSLVGLRFLPLPFVLVNYCAALAGIRPALFLAS
                     TAVGLALTVPVFTYFAALISRAASGDRSGVYLQLALALVLLALVTLLPRFWAAWKRRK
                     RYEEIKARRRTLR"
     gene            complement(3489..4574)
                     /locus_tag="VEL74_13380"
     CDS             complement(3489..4574)
                     /locus_tag="VEL74_13380"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYG63567.1"
                     /translation="MLASPMQSATTPGPTEIAASLSQRVMGQTDALREMSVALAKYLG
                     GLRVGNILMIGSSGTGKTTLMRAVEGYLASDPALAHRSTVVRIHANVLGEEAERGKPG
                     EAVLGRLLDRAREQLGPQASVETLLERARSGLVFVDEVDKIRSHVGGQANVAGIRAQE
                     ALLTLIENEAIPFALPAWMGGGSVTVDSSNLLFICAGAFEGLYDAVYDRVTIGKDRGS
                     LRPVTVVDGGRVQEALVFRLRDWLRNEDLFDYGMSPQFLSRFDAVVLLNDLGSDELVR
                     IFLETPDSAYHQTRAYFESRGIHLALSPAAVRRIAAEAARQPRLGARALKEVFRRVIR
                     DYEFDPAPYVTGGALLLDLPEVEAVLG"
     gene            complement(4585..4869)
                     /locus_tag="VEL74_13385"
     CDS             complement(4585..4869)
                     /locus_tag="VEL74_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002734303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HYG63568.1"
                     /translation="MSSFGARPEYQIVLTDSARDFYEAADAPLQRRLDRCFEILRTAP
                     HHHPNIKPLKGRFSGSYRFRSGDYRIVYRIDREGRVVIVLVIDHRSDVYG"
     gene            complement(4844..5077)
                     /locus_tag="VEL74_13390"
     CDS             complement(4844..5077)
                     /locus_tag="VEL74_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015208625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63569.1"
                     /translation="MMATDMLLRRAKDRLDSLSPERLRVADDFLAYLEERESDEATAE
                     LLQIPHFLESFERAEQEVRAGRLTPLEQLRRKA"
     gene            complement(5132..6202)
                     /locus_tag="VEL74_13395"
     CDS             complement(5132..6202)
                     /locus_tag="VEL74_13395"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HYG63570.1"
                     /translation="MTDVRAVLAGTGISIPPLEVDNHMLSRIIETSDEWVRERSGVVT
                     RYYVHPGTASSDLGVEAARAALADAGMEAGEIDYIVCATMTPDHYFPGSGTLIQQKLG
                     MAALPALDIRQQCAGFAYGLQVVDALIRSGVARNVLLVGTDVHTSLMPFSPRTFEVLH
                     GRDEGPLSDEDFQWNSRFRHLLVLFGDGAGAMIFRGEKADGRGILGSALYGDGNHRDI
                     LYVPGGGSERRPFVTPEMIASADTVPVMDGKQVFKLAVTRMPEATREVLSRAGYSTQD
                     LDLLIMHQANLRINERAQKELGLGDDKVYNNIQKYGNTTSGTLPIAFHEARQKGMAPE
                     GALVAFTALGAGLHWGSVLMRV"
     gene            complement(6240..>6712)
                     /locus_tag="VEL74_13400"
     CDS             complement(6240..>6712)
                     /locus_tag="VEL74_13400"
                     /inference="COORDINATES: protein motif:HMM:NF040439.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HYG63571.1"
                     /translation="SWLVGPIARLATPEETEAYLLLTDDAQAEAFIQRFWESRDPAPD
                     RPGNPVLEAFEQRAAEADRLYSEGGQLGRYTHRGAIFILYGEPERVTFETAPDLGSPN
                     LEVWVYGAGAPSGLDARRPANLYRFVKRGNVTVLYTPGVTDPRLRQPAILDDSR"
BASE COUNT         1016 a         2369 c         2306 g         1021 t
ORIGIN      
        1 cgacgtctac tcgccgctgg tcgccgccga cgtgccgttc atcaccaccg acgtcgagac
       61 cgccgagctg atcaagtacg cctcgaacgg cttcctcgcc accaagatca gcttcatcaa
      121 cgaggtggcg cagatctgcg aggcgtgggg ggcgaacgtc gaggtggtgg ccaagggcat
      181 gggcctcgac agccgcatcg gccccaagtt cctcaacgcc ggcccgggct acggcggctc
      241 ctgcttcccc aaggacaccc gcgccgtcgc ccagatcgcc cgcgacgccg gcagccgctt
      301 ccgcatcatc gaggcggtgc tggaggtcaa cgacgcggtc cagaagcgga tgatcaccaa
      361 gatcaccgac gccatggacg gtgtcgaggg caggaccgtg gcggtgctcg gcctgtcgtt
      421 caagcccaac accgacgaca tccgcgagtc gccggcgctg cccatcgtcc aggggctgct
      481 caacggcggc gccgcggtgc gggcgttcga tcccgaggcg atgccctact gccggccgct
      541 cttccccgcc gtcaccttct gcgacaacgc ctacgaggcg gcggaggggg cggacgcggt
      601 ggtcatcgtc accgagtgga accagttccg caagctggag ctcgaccgcc tgcaccggct
      661 gctgcaacgg ccgctggtgg tcgacctgcg caacctctac gagccggaca agatggccgc
      721 ggccggcttc gagtacgtct ccatcggccg tcccgaaggg cggccggcgg cgcaggagct
      781 gcggccggta cccgctccga tgaccgccgg ggccggaagg gcgcgcgcgt gatgcgggcg
      841 gtcatcaccg ggggcgccgg gttcctcggc tcgcacctct gcgagcgcct cctcggcgag
      901 ggctacgagg tggtctgtgt cgacaacctg gtgaccggcc gcctggagaa catcgcgcac
      961 ctccaggatc ggcccgactt caccttcctg cgccacgacg tctcgaagcc gttcgaggtc
     1021 gagggggacg tcgactgcgt cctccacttc gcctcgccgg cgtcgccgat cgactacctg
     1081 gagctcccca tccagacgct caaggtgggg tcgctcggca cccacaacac cctcggcctg
     1141 gccaagcaca agcgggcgcg ctaccttctc gcctccacct ccgaggtcta cggcgatccc
     1201 aaggagcacc cgcagaagga gagctactgg ggcaacgtca acccggtcgg gccgcgcggc
     1261 gtctacgacg aggccaagcg cttcgccgaa gcgatggcga tggcctatca ccgcgcccac
     1321 ggcctggagg tgcgcatcgt gcgcatcttc aacacctacg gcccgcgcat gcggttgcgc
     1381 gacggccggg tggtccccgc gttcatcccc caggcgctga ccaacgagcc gatgaccgtc
     1441 ttcggcgagg ggaagcagac ccgttcgttc tgctacgtcg acgacctggt cgaggggatc
     1501 tggcggctgc tcaacagcga cctcgaggag ccgtgcaaca tcggcaaccc gcatgagatg
     1561 accatcctgg agttcgccga gacgatccgc gacgcctgcg gcagcgcgag ccccatcgtc
     1621 ttcaagccgc tgccggtcga cgacccgcag acgcgccagc cggacatcac cctggcccgc
     1681 acccgcctgg gctgggagcc caaggtgccg ctgcgccagg ggctcgagag caccatcgcc
     1741 tacttccgcg aggcgctggc ccggaaccgg agtgtagaat ccgtccatgc agaatcatag
     1801 aaagtccttg gcctacgccc tcgagaagta tccggagcgt gtcgagatgg tgccgctcgg
     1861 ggagctggtg acggacagcg cgcgccagaa cccgcggcgg ccggcctacg tgacgctcca
     1921 ggtgcccgac gacgtggtca agagcctgcg cggccgggag gagagcaagg acctggtcct
     1981 cctggtccgg gtccccaagg agatcctcga acgttcggag agcctgatcg tgctccccgg
     2041 ggaggtgcgg tgatgtccga aggaaccgtg cactgcgccc gctgcggccg gaccgacgcg
     2101 ccggccctgt cccgccgtcc cctccccggc gccgccggcg acgagatccg ccagcgcgtc
     2161 tgcgccgact gctgggccga gtggctgcgc atggaggtga tggtgatcaa cgagctgcgg
     2221 ctgaacttca tggaccccgc ggcccaggag acgctgaacc agcacatgcg gcagttcctg
     2281 ttccccgccg agggcggcgg ggaagccggc ggcgggcagc tcccgggcct caacatccag
     2341 cgttgaaccc ggaggtccgc ctcgcactcc gccatcccgc cgaccgccga gccggcgccg
     2401 cggattccgc gccgcctcgt gctgcgcttc ctgctgctgg tgttgatcgt ggcggccggc
     2461 ttcgccgccc tgcgctggtc ccccctggcc gactatctca cgcaggagag gctgacccag
     2521 accttcgacc gggtgcgcga catctggtgg tcgccgctgg tgctcctcgc cgcctacctc
     2581 ctcctctgcc cgatcggcgt gccggcgacg ccgctgatgt tcaccggcgg catggtctac
     2641 ggcacgctct acggctcgat ctacaacacc ctcggcatct tcctgggcgg agcgtcgacc
     2701 ttcctcctcg gccgcggcct gggaagggag ttcgtgctgc acctggcggg caagcggatg
     2761 aagcgggtgg agcgggcgat cagccgccac gccggcttct ggagcctggt ggggctgcgc
     2821 ttcctgccgc tccccttcgt cctggtcaac tattgcgcgg cgctggcggg gatccgcccc
     2881 gccctgttcc tcgcctcgac ggcggtgggg ctcgccctga cggtgccggt cttcacctac
     2941 ttcgccgccc tcatctcccg cgcggccagc ggcgaccgct cgggcgtcta cctgcagctc
     3001 gccctggcgc tggtcctcct cgccctggtg accctcctcc cccgcttctg ggcggcctgg
     3061 aagcgccgca agcgctacga ggagatcaag gcgcgccggc gtactcttcg atagaccacc
     3121 ggaagcttcc aactcgctgc cggatgctcc ggcgagctgc cgaagattcc ggcgcgccgc
     3181 cgaagctccc gaagcgccct cggagctttc ggcgcgccgc cgtagtcttc gatggaccgc
     3241 cgggagctct gacgcaccgc cggagccttc gacagccctt cggagtgtcc cggccagccg
     3301 ccggaatctc cggcgcgctg ccggagtctc cgacgcgctg tcagagcttc cgaccgcgca
     3361 tcggagtttc cggcgcgccg ccgtagcctt cgatacgccg ttggaagctt ccaacgcgcc
     3421 gtcggagtct tcgccaagct gtcggaggct tcgacgcgcc gccgtactct ccgataagag
     3481 aaaagggcct agcccagcac cgcctcgacc tccggcaggt cgaggaggag ggcgccgccg
     3541 gtgacgtagg gggcggggtc gaactcgtag tcgcggatca cgcgccggaa gacctctttc
     3601 agcgcgcggg cccccaggcg gggctggcgg gcggcctcgg ccgcgatgcg gcggacggcg
     3661 gcgggcgaca gcgccaggtg gatgccgcgg ctctcgaagt aggcgcgggt ctggtggtag
     3721 gccgagtcag gggtctcgag gaagatgcgg accagctcgt ccgatcccag gtcgttcaag
     3781 agcaccaccg cgtcgaagcg cgagaggaac tgcgggctca tgccgtagtc gaagaggtcc
     3841 tcgttgcgca gccagtcgcg cagcctgaag accagcgcct cctgtaccct gcccccgtcg
     3901 accaccgtca ccggccgcag cgagccgcgg tccttgccga tcgtcacccg gtcgtagacg
     3961 gcgtcgtaca gcccctcgaa ggcgccggcg cagatgaaga gcaggttgct ggagtcgacg
     4021 gtgacggagc cgccgcccat ccaagcgggg agggcgaagg ggatggcctc gttctcgatc
     4081 agggtgagca gggcctcctg ggcgcggatg cccgcgacgt tggcctggcc gccgacgtgc
     4141 gagcggatct tgtccacctc gtcgacgaag accagccccg agcgcgcccg ctccagcagg
     4201 gtctcgaccg aagcctgcgg cccgagctgc tcgcgggcgc ggtccagcag ccggccgagc
     4261 accgcctcgc ccggcttgcc gcgctcggcc tcctcgccca ggacgttcgc gtggatacgg
     4321 acgaccgtcg agcggtgggc gagcgccggg tcggaggcga ggtacccctc gaccgcccgc
     4381 atcagggtgg tcttgcccgt gccggacgag ccgatcatca agatgttgcc cacccggagg
     4441 ccgcccaggt acttcgccag cgccaccgac atctcgcgca gcgcgtcggt ctgccccatc
     4501 acccgctgag acagcgaagc cgcgatctcc gtcgggccgg gagtggtggc ggactgcatg
     4561 gggctcgcga gcatcgggct cattctagcc gtagacgtcg ctacggtggt cgatgacaag
     4621 gacgatcacg acccggccct cccggtcgat ccggtagacg atcctgtaat ctccgctccg
     4681 gaaacgatag gagcccgaga acctgccctt aagaggtttg atgttggggt ggtggtgggg
     4741 tgccgtcctc aggatctcga agcaacgatc cagtctcctc tgaagaggag catccgctgc
     4801 ctcgtagaag tcccgggcag agtcagtcag gacgatctga tactcaggcc ttgcgccgaa
     4861 gctgctcaag gggagtcaac cggccggcac gaacctcctg ctcagccctt tcgaaggatt
     4921 ccaggaagtg agggatctgg agaagctctg ccgtggcttc atccgattcc cgctcttcca
     4981 gataggccag gaagtcgtcc gcgacccgca gccgctcggg ggagaggctg tccaggcgat
     5041 ccttggcgcg acggaggagc atgtcggtgg ccatcatggc tgaagtatag ctccgaggac
     5101 cggcgacatg ctccgaatcg agctcccccc ctcacacccg catcagcacc gagccccagt
     5161 ggaggccggc gccgagggcg gtgaaggcga cgagggcgcc ttcgggggcc atccccttct
     5221 ggcgcgcctc gtggaaggcg atcggcagcg tgcccgaggt cgtgttgccg tacttctgga
     5281 tgttgttgta gaccttgtcg tccccgagcc ccagctcctt ctgcgcccgc tcgttgatgc
     5341 gcaggttggc ctggtgcatg atcagcaggt ccaagtcctg ggtcgagtag ccggcacgcg
     5401 acagcacctc gcgggtcgcc tccggcatgc gggtcaccgc cagcttgaag acctgcttgc
     5461 cgtccatcac cggcacggtg tcggcggagg cgatcatctc cggcgtgacg aaggggcggc
     5521 gctccgagcc gccgccgggg acatagagga tgtcccggtg gttgccgtcg ccgtagaggg
     5581 cggagccgag gatgccgcgg ccgtccgcct tctccccccg gaagatcatc gccccggcgc
     5641 cgtcgccgaa gagcaccagc aggtgccgga agcgcgagtt ccactggaag tcctcgtcgg
     5701 acagcggccc ctcgtcgcgg ccgtgcagca cctcgaaggt gcgcggcgag aacggcatca
     5761 gcgaggtgtg gacgtcggtg ccgacgagca ggacgttgcg cgccacgccg ctgcggatca
     5821 gggcgtccac cacctgcagg ccgtaggcga agccggcgca ctgctggcgg atgtcgagcg
     5881 ccggcagcgc cgccatcccg agcttctgct ggatcagcgt gccgctgccg gggaagtagt
     5941 ggtccggcgt catggtggcg cagacgatgt agtcgatctc ccccgcctcc atgccggcgt
     6001 cggcgagcgc cgcccgcgcg gcttccaccc ccaggtccga cgacgcggtc cccggatgga
     6061 cgtagtagcg cgtcacgacg ccgctacgct cccggaccca ctcgtcgctc gtctcgatga
     6121 tccgcgacag catgtggttg tccacctcga gcggcgggat cgagatgccg gtccccgcga
     6181 gcaccgcacg aacgtcggtc atggtcgtct cctccaaaag aatcgatgaa acagcaagat
     6241 cagcggctgt cgtccaggat cgcaggctgc cggagccgcg ggtccgtcac gccgggcgta
     6301 tagagcacgg tgacgttgcc gcgcttgacg aagcggtaga ggttcgcggg ccgccgggcg
     6361 tcgagccccg agggcgcccc ggcgccgtag acccagacct cgaggttggg gctgccgagg
     6421 tcgggggcgg tctcgaaggt caccctctcg ggctcgccgt agaggatgaa gatcgccccc
     6481 cggtgggtgt agcggccgag ctggcccccc tcgctgtaga ggcggtcggc ctcggcggcg
     6541 cgctgctcga aggcctcgag cacgggattg cccggacggt ccggcgccgg gtcccggctc
     6601 tcccagaagc gctggatgaa ggcctcggcc tgggcgtcgt cggtcaggag caggtaggcc
     6661 tcggtctcct cgggtgtcgc gaggcgggcg atggggccca ccagccagct cg
//
LOCUS       DASPJI010000492         5390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_376671, whole genome shotgun sequence.
ACCESSION   DASPJI010000492 DASPJI010000000
VERSION     DASPJI010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376671"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VEL74_13405"
     CDS             <1..215
                     /locus_tag="VEL74_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63572.1"
                     /translation="AATRVWAAVESLRKAGVPHGAPLTLHPGGGDGWLLLRSGDLLIG
                     TLLAPVHELSGPAALAVLLEAGHEGV"
     gene            202..621
                     /locus_tag="VEL74_13410"
     CDS             202..621
                     /locus_tag="VEL74_13410"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017742125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HYG63573.1"
                     /translation="MRAYEYRHLVGFEETNLVGNVYYVNHLRWQGRCREMFLRDHAPE
                     VLADLSNGLALATVRCACDYLAELEAFDELLIRMRLGGIAQNRITLLFEYWRTNHGPE
                     ELIARGEQQIACMRRDGHRTVPTPIPPGLREALKAYE"
     gene            complement(612..2018)
                     /locus_tag="VEL74_13415"
     CDS             complement(612..2018)
                     /locus_tag="VEL74_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63574.1"
                     /translation="MDDLSRAGPAVKDIAGTAGRFGRGDLGTALLLALLAFLVYNANL
                     RLIATGDSLPARFLPFAVWTEGSLYLDPVLKVTVRDHPAPYWILRTRDGHIGSMYPVV
                     APLLVTPLYAPAVLYLDAKGWNDERLARIGKIMEKVSASVVASVCVALMYLALRRRAG
                     RPMALLLTLAFALGTNTWMTSSQALWQHGVTELLLLAALLCFTGRPSWAVLVTAGAAC
                     GLLIANRLADAPLAAALAAFSPFWARRRWPVFAAAAAVPLALLVTYNLSTFGLLGGGY
                     AVVLREKGGGFFSFPILPGLAGLLMSPGKGLFVFSPFLAALPVFWRRTVADERYRLLT
                     LCLAGGVLLELLAYSRIDWRGGSSYGPRYLSSMLPVVVWMLPPVVAALGRMGRAVFLV
                     LVLFSAGVQAIGAFCYRGHSDGLFNQAPQTDLFGVTWRLGNVPFVLDPQYSGCAPGDL
                     LELVQEEARSGGRSAPYS"
     gene            complement(2633..3595)
                     /locus_tag="VEL74_13420"
     CDS             complement(2633..3595)
                     /locus_tag="VEL74_13420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HYG63575.1"
                     /translation="MTSLWTRGGLTWRELGVRLWRQIYEDELFGRCAELAYFFLFSIF
                     PLLLFLTTLLGYLAGASTELRWNLLLYISRVSPSPEITRLISDTLNEITLGRGGAKLS
                     ISLLIALWVASNGMIAVSRTLNTACGLRETRRWWRRRLIAMALTIAFAVLIICALVLI
                     FYGGESVDRLADRFGLGPFLPLLWRVFRWPLILTFVVISFEMIYNYAPNLGDSPSRQW
                     WTPGAFTGVALFLLASFALRIYLAYFYIYSRAYGSLGAVILTLVWFYLIAFAILMGGE
                     VNSEIAKEISRRKELEREAARARGEEVPEKPAKRRRFPGRRRAR"
     gene            3674..>5390
                     /locus_tag="VEL74_13425"
     CDS             3674..>5390
                     /locus_tag="VEL74_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63576.1"
                     /translation="MSDLDTVLDPSRSAHASIQGYLYQAYLGVERWLELKEDEVLRCE
                     GDEDLDRLLRDGSGGWSEQVKAYTGDVNIRDEVVQDTLRRFLVSHHRLRTGDKPDLRR
                     FLFTSTATRRRQVTDERYPVDVLAVWEEAKEDGAVRSQVVGVLRTVLPLEETADRPAS
                     RRFAEQVRAALAWLDGDAERWADLIGSLEWRLGEPQLTEVRGKIDQALNHVRPGAPPN
                     LRDRLLSEVLDASIAPDPRDRCRTREQLVALLDQAEAEVARWLATARGYGLVKRLEEL
                     DDLRGILVDKPQDLPPSPRPGQLLTAAYEVIPFDETGREEILNDLAGWCADSRLRLVR
                     LIHGDGGLGKTRLLIEWCRRLAAQGWLTGFLLPVATPDELPRLLQGTLPRLVVIDYAE
                     TRLEAVRALVMRLAYLKDGPKVRLVLLARREGDWWESLKKDAATEDLLDSTPPRDLPR
                     LLPGLEERRQAFRTAAHAFAQHLLYRTGIEGAKVHGAVDPREVDQREVELGVVVPPPL
                     GGRAVGSGGGQEGGSSRRGLAPKPPTRGEDTQETRRLSPPQSSPPPNLPHSLPRPLPG
                     GEAPPP"
BASE COUNT          927 a         1846 c         1876 g          741 t
ORIGIN      
        1 ccgcggcgac ccgggtctgg gcggcggtcg agagcttgag gaaggcgggg gtgccgcacg
       61 gcgcgccgct gaccctccat ccaggtggcg gtgacggctg gctcctgcta cgctcggggg
      121 acctgctgat cgggaccctc ctggcaccgg ttcacgagct gtccggcccg gccgccctgg
      181 cggtcctctt ggaggccgga catgagggcg tatgagtacc gacacctcgt cggattcgaa
      241 gagaccaacc tcgtcggcaa cgtctactac gtgaaccacc tgcgctggca ggggcgttgc
      301 cgcgaaatgt tcctccgcga ccacgccccg gaggtcctgg cggacctctc gaacggcctg
      361 gctctggcga cggtccgctg cgcctgcgac tacctggccg agctggaagc cttcgacgag
      421 ctgctgatcc gcatgcgcct ggggggcatc gcccagaacc gcatcaccct tctcttcgag
      481 tactggcgca ccaaccatgg ccccgaggag ctgatcgcga gaggcgagca gcagatcgcc
      541 tgcatgcgcc gcgacggcca ccgcactgtc ccgacgccca tccccccggg tctccgcgaa
      601 gccctcaagg cttacgagta gggtgcgctg cgcccgccgg agcgggcctc ctcctggacc
      661 agctcgagga ggtcccccgg cgcgcagccg gaatattgcg gatcgaggac gaacggcacg
      721 ttccccaggc gccaggtcac gccgaacagg tccgtctgcg gcgcctggtt gaacaacccg
      781 tcgctgtgcc ctcgatagca gaaggcgccg atggcctgga cgccggccga gaacagcacc
      841 aggaccagga agaccgcacg gcccatccgg cccagcgccg ccaccaccgg cggcagcatc
      901 cagacgacga cgggtagcat cgaggacagg tagcgcgggc cgtaggagga gcctccccgc
      961 cagtcgatcc tgctgtaggc gaggagctcg aggaggacac cgccggcgag acagagggtc
     1021 aggagacggt atcgctcgtc cgccacggtg cggcgccaga agaccggcag cgccgccagg
     1081 aaggggctga agacgaagag ccccttgccc gggctcatca gcaatccagc caggcccggc
     1141 aatatcggaa acgagaagaa ccctccccct ttctcccgga ggacaacggc gtagccccct
     1201 cccagcaggc cgaaggtgct caggttgtac gtcaccagca gcgccagcgg cacggccgcg
     1261 gccgcggcga agacaggcca ccggcggcgg gcccagaacg gcgagaaggc ggcgagggcg
     1321 gcggcgagcg gagcgtcggc caggcggttg gcgatcagca ggccgcaggc ggcgccggcg
     1381 gtgaccagga cagcccacga cggcctgccg gtgaagcaca gcaacgctgc cagcaggagc
     1441 agctcggtca cgccgtgctg ccacaaggcc tggctgctcg tcatccaggt gttggtgccc
     1501 aaggcgaagg ccagggtcag cagaagggcc atgggcctcc cagcgcggcg gcgcagggcc
     1561 aggtacatca gggccacgca gacgctggcg accaccgagg ccgagacctt ctccatgatc
     1621 ttgccgatcc gcgccagacg ctcatcgttc caccccttcg cgtcgaggta cagcaccgcc
     1681 ggagcgtaga ggggggtcac caggaggggc gcgaccaccg ggtacatcga accgatgtgg
     1741 ccgtcgcgcg tccgcaggat ccagtacggg gccggatggt cgcggaccgt caccttcagc
     1801 accgggtcga ggtagaggct cccctcggtc cagacggcga agggcaggaa gcgtgcgggc
     1861 agggagtcgc cggtggcgat gagacgcagg ttggcgttgt agacgaggaa ggcgagcagg
     1921 gcgagcagca gggcggtccc gagatctccc cgcccgaaac gcccggccgt gcccgcgatg
     1981 tccttgacag cggggccagc gcgggacaga tcgtccatct gcgagctcac taactttgat
     2041 tgtatcctta gatgagactc aatgagaatc tcaggatcag gtcccgggag cgccgctcga
     2101 gtcaccgcgc ggtccatgga gtcctggcgg cgggaacgct ggagcctgcg cctgtaggcg
     2161 gacggctccg gtctgagccg caacgcctcc tccctctttc ccgttggttt taccgcgagc
     2221 ctggtcgcga gtcccgggag attcgcaacg tggctcgcgg cagttcgaaa ccccctcgcg
     2281 agatacgaaa cagaacccgc ctccgcggtt tcgagtccac ttccattcgc gacggcgccc
     2341 ttgggctccg tctcgaatcc atcggcactc gcgacggcag gagcggcata cggaacgccg
     2401 cttcgcgacg gcggacctcc cgaagcggga ttcgcgacgg cagtcgggag attcgtcgcg
     2461 agtcccgcgg gtttcgtcgc gaatcaacct tctctcgtcg cgaatgcatc tcgtttcgtc
     2521 tcgaaacccc tcgcggtggt cgcgagatgg agggggtccg ttgtgagtga ggcgggattc
     2581 gcgacagccg cctcttcact cgcgacacgc cagggagccg cgacagcgtc ggtcaccgcg
     2641 cgcgccgccg cccggggaac cgccggcgct tcgcgggctt ctccggcacc tcctcccccc
     2701 gcgcccgcgc cgcctcccgc tccagctcct tgcggcggct gatctccttg gcgatctcgg
     2761 agttcacctc ccctcccatc aggatggcga aggcgatcag atagaaccag acgagggtca
     2821 ggatgaccgc tcccagggag ccgtaggcac gggagtagat gtagaagtag gcaagataga
     2881 tccgcagggc gaaggaggcg aggaggaaga gggcgacgcc ggtgaaggct cccggggtcc
     2941 accactgccg gctggggctg tcccccaggt tgggggcgta gttgtagatc atctcgaagg
     3001 agatgaccac gaaggtcagg atcagcggcc accggaagac ccgccagagg agggggagga
     3061 aggggccgag gccgaagcgg tccgccaggc ggtcgacgct ctcgccgcca tagaagatga
     3121 ggaccagggc gcagatgatg agcacggcga aggcgatggt cagcgccatg gcgatcagcc
     3181 gccgccgcca ccagcgccgc gtctcccgca ggccgcaggc ggtgttgagc gtccgcgaga
     3241 cggcgatcat gccgttcgaa gccacccaca gggcgatcag gagggagatc gacagcttcg
     3301 cgccgccgcg gcccagggtg atctcgttca gggtgtcgct gatcagccgc gtgatctccg
     3361 ggctcgggga gacccgggag atgtagagga ggaggttcca gcgcagctcg gtgctggcgc
     3421 cggcgagata gccgaggagc gtcgtcagga agaggagcag ggggaagatc gagaagagga
     3481 agaagtaggc cagctcggcg cagcgcccga acagctcgtc ctcgtagatc tggcgccaga
     3541 ggcgcacccc cagctcccgc caggtcaggc cgccgcgtgt ccataaagag gtcatggtcg
     3601 tcctccccgc tcccagcctg gagagtatca gggagcgcgg aggagggggc tccgctaaaa
     3661 tactccccac cccgtgagcg acctcgacac cgtcctcgac ccgagccgat cggcccatgc
     3721 ctcgatccag ggatacctgt atcaggccta tctcggcgtc gagcgctggc tggagctgaa
     3781 ggaggacgaa gtcctccgct gcgaggggga cgaggacctc gaccggctgc tccgcgacgg
     3841 ctcgggcggc tggagcgagc aggtcaaggc ctacaccgga gacgtcaata tccgcgacga
     3901 ggtcgtccag gacaccctgc gccgcttcct cgtctcccac catcgcctcc gcacggggga
     3961 caagccggac ctccggcgct tcctcttcac ctccaccgcc acccggcgaa ggcaggtgac
     4021 cgacgaacgc tatcccgtgg acgtcctggc ggtctgggag gaggccaagg aggatggcgc
     4081 ggtgcggagc caggtggtcg gcgtcctccg gacggtcctc cccctggaag agaccgcgga
     4141 ccggccggca agccgccgct tcgccgaaca ggtccgggcg gccctcgcct ggctggacgg
     4201 cgacgccgag cgctgggcgg acctcatcgg gtccctcgag tggaggctcg gggagccgca
     4261 gctcacggag gtgcgcggca agatcgacca ggcgctgaac cacgtccgtc ccggcgcgcc
     4321 gccgaatctg cgggaccgcc tcctctccga ggtgctggac gcctccatcg cccccgatcc
     4381 aagggaccgt tgccggaccc gggagcagct cgtggccctg ctcgaccagg ccgaggccga
     4441 ggtcgcccgg tggctggcga cggctcgcgg atatgggttg gtgaagaggc tggaggagct
     4501 ggatgacctc cgcggcatcc tggtcgacaa gccgcaggat ctccctccct cgccccgtcc
     4561 cggtcagctc ctcaccgccg cctacgaggt catccccttc gacgaaaccg gccgcgagga
     4621 gatcctgaac gatctggccg gctggtgcgc cgatagccgc ctgcggttgg tccgcctgat
     4681 ccatggcgac ggcgggctcg gcaagacacg gcttctgatc gaatggtgcc gccgtctggc
     4741 cgctcagggt tggctcaccg ggttcctcct gcccgtggcc actccagacg agctgccccg
     4801 tctccttcag ggcactctcc cccgcctcgt ggtcatcgac tacgcggaga cccggctcga
     4861 agccgtccgc gcgctggtga tgcggcttgc ctatctaaag gacgggccca aggtccgcct
     4921 cgtcctcctc gcccggcgtg agggagattg gtgggagtcc ctgaagaagg atgccgcgac
     4981 ggaggatctc ctggacagca cccctccccg cgacctgccg cgcctcctcc ccggcctcga
     5041 agagcgccgc caggccttcc ggaccgccgc gcacgccttc gcccagcacc tgctctacag
     5101 gaccgggatc gagggggcga aggtacacgg ggcggtggat ccgagggagg tggatcagag
     5161 ggaggtggaa ctaggggtgg tggtgcctcc ccccctgggt gggcgggcgg tagggagcgg
     5221 gggaggtcag gaggggggtt cttctcggcg cggcctggca ccaaagccgc ctacaagagg
     5281 agaggacacc caagagacca ggaggctctc gcctcctcaa agcagccccc ctcctaacct
     5341 cccccactcc ctaccacgcc ccctcccagg gggggaggca ccaccacccg
//
LOCUS       DASPJI010000493         8851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_29457, whole genome shotgun sequence.
ACCESSION   DASPJI010000493 DASPJI010000000
VERSION     DASPJI010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29457"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VEL74_13430"
     CDS             <1..252
                     /locus_tag="VEL74_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HYG63577.1"
                     /translation="RGGEGAIRELIFLEPMSLSLLTQHRVEAPYGLAGGGPGLPGRQR
                     LVRATGEVVDLAPVDGCEVGPGDRLILETPGGGGWGTAD"
     gene            complement(206..730)
                     /locus_tag="VEL74_13435"
     CDS             complement(206..730)
                     /locus_tag="VEL74_13435"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HYG63578.1"
                     /translation="MPDRPSDLHVVLVEPEIHWNTGNAGRTCLAAGARLHLVEPLGFS
                     LDEREVRRAGLDYWPRVEPRIWPSWPDFETRLPDLGEPFFFSAEGTRSFWEVSYPERV
                     VLVFGRESVGLPREIRDSYRDRLLAIPMLDRGIRSLNLSTSVALAAYEVMRQRGRSGV
                     NPPYPNLPRPGSPE"
     gene            complement(763..1401)
                     /locus_tag="VEL74_13440"
     CDS             complement(763..1401)
                     /locus_tag="VEL74_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63579.1"
                     /translation="MKKPILIAAAVLVSTLPLSADETIRTLNERYAAGDVKSLYLDVP
                     VGEVVVQGGGASQVTVEVELECDSDRSRCAELAKAVRLQGGKKGDRLQVELDNWPKGN
                     GQGLQANVRVTMPRDLALQTDLGVGELRISGIEQDVNADVGVGEVSVTMPESAVRSVN
                     LDSGVGDATLRTSEKRIQGSGMVGKELKWNKGTGSADVRVDCGVGEIGVSLQ"
     gene            1536..2297
                     /locus_tag="VEL74_13445"
     CDS             1536..2297
                     /locus_tag="VEL74_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63580.1"
                     /translation="MSRQSGGLGPLARLLRPFLGGKGGNPWPPFWVLRQEVPLAGAEQ
                     ELEAATAEIMGEVQRRKPASGTFHVTTLRLDGGDHLFLMPLRGPAGLADLMSEWNRLA
                     MEVGASRWGEMAARGNRAMETFSLRVFMEPPGLGWRPEGAPGLGDRSGAFLFDFFHLR
                     PVAEQQFGPVVRELATVCRDRVPGGFTVLLCTAGAELPLVVLVWTAPDAAELQRREEA
                     LAAAGGAAILERLEGLARRRERRRGELRPQLSHLP"
     gene            2363..3394
                     /locus_tag="VEL74_13450"
     CDS             2363..3394
                     /locus_tag="VEL74_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HYG63581.1"
                     /translation="MPPTPSTFQEAPPSSRPIRDLGLTIAGTSLEPVIAELEAELEQA
                     GIRRVRPRFYLSTEWGVPFGTVAIAIPFYLARPDLTALHAERTSHVEGMGRADLLRYL
                     RHEMGHVVNYAYLLYEDEDWVRHFGSMTQPYLEEYRPEPFSRRFVRHLPGWYAQKHPD
                     EDWAETFAVWMTPGCRWEEQYATWTKALEKLRYCDRVMAGLRDRDPLVTADELDEDVG
                     EISYTLEDYYKYMDAADPELPPGLDGALTTIFEDLGRPEDLTTDAPRLSAAELIRRLT
                     RLLPADVFRWTGHFPERTRLLLSHLAERADDLRQVYPADREQEAVVALTTLVTALAMS
                     HVHRGSYMP"
     gene            complement(3345..4673)
                     /locus_tag="VEL74_13455"
     CDS             complement(3345..4673)
                     /locus_tag="VEL74_13455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HYG63582.1"
                     /translation="MKLRARRVLLALAWLAAALAAGRIASMAWHHFDRQERADGNRGH
                     ATIDFGGSWTMGRMLVEGRGSELYEREALREVLEASYGKEDAAKLLSWFMPGGTRDGR
                     AITGPLYPPINAFVTAPLSLLPPQTAYRVHQIADLLLLVLTALGVTVLSSGRIWLPVA
                     VLLIALFPGFQGNVALGQNSVLSLAVLVWGWVLMARSRDTGGGALWGLLAYKPVWAAS
                     FFLVLPLARRWRAAVAMGAVGAAQIALTLPFVGVHSWLDWLAIGRQAAARYGLDRRWI
                     ELSRDLAGIPRRFLLDFTVPMHRRGDPAADMAALALWLGVLAVTVAVALRHRGRMRAV
                     AGPPAAFLLLGGWLLCYHFMYYDALLAFLPVILLFTEPGVPPRERLAPALALAALFFI
                     ENPLRYLGYGDEGQPWSTYVLLLLWAWCGWKTARPTACTSRGARGSSPAR"
     gene            complement(4683..5657)
                     /locus_tag="VEL74_13460"
     CDS             complement(4683..5657)
                     /locus_tag="VEL74_13460"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HYG63583.1"
                     /translation="MNITILTGLESADPRSYDVVVGQVASALKKKGHKVSILGVHDDL
                     PKMVQGLNRRRPELVFNLLESFGDNLNADVAVAGVLELLRLRYTGSGPGELFLRQDKG
                     LAKKVFAFEGISYPHFAVFGKDADLETAGQLRMPLFVKPLRADASIGIDGDSLVRDAI
                     ALMKRVVAIHEKVGDSALVEEYIEGREFYVGVLGNREPLALPPIEMDFSGMPEGMPHI
                     LGSKAKWSPKSAEYKGTQSVLAEIPDELRARLQKVAVDAYRSLRARDYGRVDLRLTET
                     GDIYVIEVNANCYLEEGSEFATAAKAAGIEFPDLVQRIVDLALERYAD"
     gene            5777..7234
                     /locus_tag="VEL74_13465"
     CDS             5777..7234
                     /locus_tag="VEL74_13465"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HYG63584.1"
                     /translation="MVEYLRSRASIAALVACSLLMVSCSSNPATGKRQLAFISEEQEV
                     AMGREADQQVVQSLGLYQDEEVQAYVNRLGQQLARESERPNLPWTFRVVDDPVVNAFA
                     FPGGFIYVTRGLMTHLSNEAELVSVLGHEIGHVTGRHSVEQMSKQQLAQIGFIAGMLI
                     KPELAQYGDLAQTGLGLMFLKYGRDDEREADDLGLRYMVRENYDPREMPQVFDVLRRV
                     SEQQGGGRVPGWMSTHPTPENRIERIESQLASLGSDSSNRIVNRERYLKTIDDLPFGQ
                     NPREGYFRGNQFVHPELAFSITFPQGWQMSNEKQAVGAISPRQDAIVVLSLAQGSSPQ
                     EAAQQFFSQQGVQQGQALRANLGGLPAVANEFAANTQQGTLVGLAAFVEDRDRVYRIL
                     GYAPQNSWRGNESAISNALASFERVTDRRLLNVTPKRLDVVTVPSAMSLEEFNRRYPS
                     TVDIQTLAIINQANADTRFPAGTEVKRVVGGEVPS"
     gene            7386..7757
                     /locus_tag="VEL74_13470"
     CDS             7386..7757
                     /locus_tag="VEL74_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYG63585.1"
                     /translation="MKYLLLAYHDETKWDALSDDEKDAIGSQCLPYDEELRQGGHVIA
                     MGSLEHTRMSTVMRTRSGRLLTTDGPFIETKEQLGGFLIIEARDLNEALQVAANHPAA
                     RMNERLGWCVEVRPFEFFEQP"
     gene            7794..8084
                     /locus_tag="VEL74_13475"
     CDS             7794..8084
                     /locus_tag="VEL74_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018301017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HYG63586.1"
                     /translation="MCRNIKTLFNFDPPATDEEVRAASLQFVRKICGFTSPSKANEAA
                     FDLAVEQVAESARALLASLVTSAPPHDREIEAAKARARSLQRFGGAAGAGGS"
     gene            8135..>8851
                     /locus_tag="VEL74_13480"
     CDS             8135..>8851
                     /locus_tag="VEL74_13480"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HYG63587.1"
                     /translation="MLLAREDATPLLAVERLAGLQAQMARPPFVGLWTRLAGFRRDGL
                     LAPLHRREIVRVTAMRATLHLLTAADYVALRGAFQPVLAKAMQSILRARSAEVDLARI
                     EAEARSFFGEGAATFDAFRTHLKAKYPEGDERAMAYAIRTHLPLVQVPGEQAWGFPPA
                     PEFALAEPWLKELKELKKQGKKIPMEPGPPHELVRRYLAAFGPATPGDAQTWSGLSGL
                     REVFESLRPELVTFRDARKRE"
BASE COUNT         1382 a         3209 c         3017 g         1243 t
ORIGIN      
        1 cggggcggcg agggcgccat ccgcgagctg atcttcctgg agcctatgtc cctctcgctc
       61 ctcacccagc accgggtgga ggcgccctat ggcctcgcag gagggggccc gggcctcccc
      121 ggccggcagc gtctggtgcg cgctacgggg gaggtcgtcg acctggcgcc cgtcgatggc
      181 tgcgaagtcg gccccggcga ccggctcatt ctggagaccc cgggcggggg aggttggggt
      241 acggcggatt gaccccgctc cggccccgct gccgcatcac ctcgtaggcc gccagcgcga
      301 cggaggtgga gaggttgagg gagcggatgc cgcgatcgag catggggatg gcgaggaggc
      361 ggtcccggta agagtcgcgg atctcccgcg gcaaccccac gctctccctc ccgaagacca
      421 ggaccacccg ctcggggtag gagacctccc agaagctccg cgtcccctcg gcactgaaga
      481 agaagggctc tccgagatcc ggcagccgcg tctcgaagtc gggccaggag ggccagatcc
      541 gcggctccac ccgcggccaa tagtccaggc cggcgcggcg gacctcccgc tcatccagcg
      601 agaagccgag tggctccacc aggtggagcc gggcgccggc cgcgaggcag gtgcggccgg
      661 cgttgccggt gttccagtgg atctccggct ccaccagcac cacgtgcaga tcggaagggc
      721 ggtcgggcat gccgggccgc agcatacccg acagccggcg ctctactgga gcgacacgcc
      781 gatttcaccg acgccgcagt ccacccgcac gtcggccgag ccggtgccct tgttccactt
      841 gagctccttg ccgaccatac cggagccctg aatccgcttc tccgaggtcc gcagggtggc
      901 gtcgcccacg ccggaatcga gattgaccga gcgcaccgcc gactcgggca tggtcaccga
      961 gacctcaccc acacccacgt cggcgttgac gtcctgctcg atgccggaga tccgcagctc
     1021 gccgacgccc agatcggtct ggagagccag gtcgcgcggc atggtcaccc tgacgttggc
     1081 ctggagcccc tggccgttgc ccttgggcca gttgtcgagc tccacctgca ggcggtcccc
     1141 cttcttgccc ccctgcaggc ggaccgcctt ggccaactcg gcgcagcggc tccggtccga
     1201 gtcgcactcg agctccacct cgaccgtgac ctggctcgct ccccctccct ggacgaccac
     1261 ctcgcccacc gggacgtcga gatagaggct cttcacatcc ccggcggcgt agcgctcgtt
     1321 tagggtccgg atcgtctcgt cggcactcag cggcagcgtg ctcaccagca cggcggccgc
     1381 gatcaggatc ggcttcttca tgattttttc tcctcccatc agtgggccac ctctcgggcc
     1441 tgaccccact acgcaggaga gccgccgcgg gttccaaact ttcttgcgtc ccgcgcgaga
     1501 ggcggccgtg caccaccctg ttaggaggta gctcgatgag tagacaatcc gggggactcg
     1561 gtcccctcgc ccggctcctg cgcccgttcc tgggaggaaa gggaggcaac ccctggcctc
     1621 cgttctgggt gctccgccag gaggtgccac tggcaggggc cgagcaggag ctggaagcgg
     1681 cgacggccga gatcatggga gaggtccagc gccgcaagcc ggccagcggc acgtttcacg
     1741 tcaccaccct gcggctcgac gggggagacc acctcttcct catgccgttg cgcggccccg
     1801 ccgggctcgc ggacctcatg tcggagtgga accgcctggc aatggaggtg ggcgccagcc
     1861 gctggggcga gatggcggcc cggggcaacc gggcgatgga gaccttcagc ctccgtgtct
     1921 tcatggagcc tccgggtctg ggctggcggc ccgagggggc tccgggtctg ggggaccgtt
     1981 ccggcgcctt cctcttcgac ttcttccacc tgcggccggt ggcggagcag cagttcgggc
     2041 cggtggtgcg ggagctggcc accgtctgcc gcgaccgcgt gccgggaggc ttcaccgtcc
     2101 tcctctgcac cgccggggcc gagctgccgc tcgtcgtcct ggtctggacg gctcccgacg
     2161 ccgccgagct gcaacggcgg gaggaggccc tcgccgccgc cggaggcgcg gcgatcctgg
     2221 aacgtctgga agggctggcg cggcgccggg agaggaggcg gggagagctc cggccccagc
     2281 tctcgcacct cccctgaggc cagagcggcg gcgtggcggc gcggcaccga tcttgcctct
     2341 cgaaccggcg aggagccgtg ccgtgccgcc gaccccgtcc accttccagg aagcgccccc
     2401 gtcctcccgg ccgatccggg acctggggct caccatcgcc gggacctctc tggagccggt
     2461 gatcgccgag ctcgaagccg agctcgagca ggccggcatc cgccgggtgc gtccccgctt
     2521 ctacctctcg accgagtggg gggtcccctt cggcacggtg gccatcgcca tccccttcta
     2581 cctggcgcgg ccggacctga ccgccctgca cgccgagcgc accagccacg tcgaggggat
     2641 ggggcgggcc gacctcctgc gctacctccg gcacgagatg gggcacgtgg tcaactacgc
     2701 ctatctcctc tatgaggacg aggactgggt gcggcacttc ggctccatga cccagcccta
     2761 cctcgaggag taccggcccg agccgttcag ccgccgcttc gtccgccacc tgcccggctg
     2821 gtacgcccag aagcacccgg acgaggactg ggccgagacc ttcgccgtct ggatgacccc
     2881 cggctgccgg tgggaggagc agtacgccac ctggaccaag gcgctggaga agctccggta
     2941 ctgcgaccgg gtgatggccg gcctgcggga ccgcgatccc ctggtcaccg ccgacgagct
     3001 ggacgaagac gtgggggaga tctcttatac cctggaggac tactacaagt acatggacgc
     3061 cgccgacccc gagctcccgc cgggcctgga cggggccctg accaccatct tcgaggatct
     3121 ggggcggccc gaggacctga ccaccgacgc gccgcgcctc tcggcggcgg agctgatccg
     3181 gcgcctcacc cgcctcctcc cggccgacgt cttccgctgg accggccact tcccggagcg
     3241 cacccgcctc cttctctccc atctggccga gcgcgccgac gacctccgcc aggtctaccc
     3301 ggcggaccgc gagcaggagg ccgtcgtcgc cctcaccaca ctggtcaccg cgctggcgat
     3361 gagccacgtg caccgcggct cgtacatgcc gtaggccgcg ccgtcttcca gccgcaccag
     3421 gcccacagga ggagcagcac gtaagtgctc cacggctgcc cctcgtcccc gtagccgaga
     3481 tagcgcaggg ggttctcgat gaagaacagg gccgcgagcg ccagcgccgg ggccagccgc
     3541 tcccggggag gcacgccggg ctccgtgaac agcagaatca ccggcaggaa ggcgagcagc
     3601 gcgtcgtagt acatgaaatg gtagcagagc agccaaccgc cgagcagcag gaaggcggcc
     3661 ggcggcccgg ccacggcgcg catccggccg cgatggcgca gcgccaccgc gacggtgacg
     3721 gcgagcaccc ccagccagag cgccagcgcg gccatgtccg ccgccgggtc gccccgccgg
     3781 tgcatcggca cggtgaagtc caggaggaag cgccggggga tgcccgcgag gtcgcggctg
     3841 agctcgatcc agcgccggtc caggccgtac cgcgccgccg cctgccggcc gatcgccagc
     3901 cagtcgagcc acgagtggac ccccacgaag ggcagggtga gggcgatctg cgccgcgccg
     3961 acggccccca tggcgaccgc ggcgcgccag cgccgggcga gcggcaacac caggaagaag
     4021 gacgcggccc agaccggctt gtaggccagc aggccccaaa gcgcgccgcc tccggtgtcg
     4081 cggctacgtg ccatgagaac ccacccccag accaggaccg ccagcgacag gacgctgttc
     4141 tggcccagag cgacgttccc ctggaagccg ggaaagagcg cgatcagcag gaccgccacc
     4201 ggcagccaga tccgtcctga ggagaggaca gtgaccccca gggcggtcag caccaggagg
     4261 agcaggtccg cgatctggtg gacgcggtag gcggtctgtg gcggcaggag ggagagcggg
     4321 gccgtcacga aggcgttgat cggcggatag agcggcccgg taatggcgcg tccatcccgg
     4381 gtgcccccgg gcatgaacca ggacaggagc ttggcggcgt cctccttccc gtagctcgcc
     4441 tctagcacct cccgtaacgc ctcgcgctca tagagctcgg agccccggcc ctccaccagc
     4501 atcctcccca tggtccacga gccgccgaaa tcgatcgtgg cgtggccgcg gttgccgtcg
     4561 gcccgctcct gccggtcgaa gtggtgccag gccatggagg cgatgcgccc ggcggccagc
     4621 gcggccgcca gccaggccag cgccaggagc accctccgag ctcggagctt cacgggggaa
     4681 gactaatcgg cgtagcgctc cagcgccaga tccacgatgc gctggacgag gtcggggaac
     4741 tcgatgcccg cggccttggc ggcggtggcg aactcgctgc cctcctccag gtagcagttg
     4801 gcgttgacct cgatgacgta gatgtcgccc gtctcggtca gccggaggtc gacccgcccg
     4861 tagtcgcggg cccgcaggga gcggtaggcg tcgacggcga ccttctgcag gcgggcccgc
     4921 agctcgtccg ggatctcggc gaggaccgac tgcgtcccct tgtactcggc gctcttcggc
     4981 gaccacttgg ccttggagcc caggatgtgc ggcatcccct cgggcatgcc ggagaagtcc
     5041 atctcgatcg gcggcagggc caggggctcc cggttgccca gcacgccgac gtagaactcc
     5101 cggccctcga tgtactcctc gaccagggcg ctgtccccca ccttctcatg gatggccacc
     5161 acccgcttca tcagggcgat ggcgtcgcgc accagggagt cgccgtcgat gccgatggag
     5221 gcgtcggcgc gcagcggctt gacgaagagc ggcatgcgga gctggccggc ggtctcgagg
     5281 tccgcgtcct tgccgaagac ggcgaagtgg ggataggaga tgccctcgaa ggcgaagacc
     5341 ttcttggcga gccccttgtc ctggcgcagg aacagctcgc cggggccgct gccggtgtag
     5401 cgcagccgta acagctcgag gacgcccgcc acggccacgt cggcgttcaa gttgtcgccg
     5461 aagctctcca ggaggttgaa caccagctcg ggccgccgcc ggttgagccc ctgcaccatc
     5521 ttcggcaggt cgtcgtgcac gccgaggatg gacaccttgt gccccttctt cttgagcgcc
     5581 gaggcgacct gccccaccac cacgtcatac gagcggggat ccgcgctttc gagccccgtc
     5641 aggatcgtta tattcatgcc gcgccgagac atgcaaggcc cgggccgccc ggcattcctc
     5701 ttgcagtacc ccctttcgac cgcgccggga ggccccattc ggggagccgg cctaacgaga
     5761 aaacgaggag gcagccatgg tcgaatacct tcgcagcaga gcatcgatcg cggcgctcgt
     5821 ggcctgctct ctcctgatgg tgagctgctc cagcaatccg gcgactggca agcgccagct
     5881 cgcgttcatc agcgaagagc aagaggtcgc gatgggccgc gaggcggacc agcaggtggt
     5941 ccaatccctc ggcctctacc aggacgagga ggtccaggcc tacgtcaacc gcctgggcca
     6001 gcagctcgcc cgcgagtccg agcgccccaa cctgccgtgg accttccggg tggtggacga
     6061 tccggtggtg aacgcgttcg cgttccccgg cggcttcatc tacgtgaccc gcggcctgat
     6121 gactcatctc tccaacgagg ctgagctggt ctcggtgctg gggcacgaga tcggccacgt
     6181 gaccggccgc cacagcgtcg agcagatgag caagcagcag ctcgcgcaga tcggcttcat
     6241 cgccggcatg ctcatcaagc ccgagctggc gcagtacggc gacctggcgc agaccggcct
     6301 cggcctcatg ttcctgaagt acggccgcga cgacgagcgc gaggcggacg acctcggcct
     6361 gcgctacatg gtgcgggaga actacgaccc gcgcgagatg ccgcaggtgt tcgacgtgct
     6421 gcggcgcgtc agcgagcagc agggcggcgg ccgcgtgccg ggctggatgt cgacccaccc
     6481 gacgccggag aaccggatcg agcggatcga gtcgcagctc gccagcctgg gcagcgactc
     6541 ctccaaccgc atcgtcaacc gcgagcgcta tctcaagacc atcgacgacc tgccgttcgg
     6601 ccagaacccg cgcgagggct acttccgggg caaccagttc gtccatccgg agctcgcgtt
     6661 cagcatcacc ttcccgcagg gctggcagat gagcaacgag aagcaggcgg tgggcgccat
     6721 cagcccgcgc caggacgcca tcgtggtcct gagcctcgcc cagggcagct cgccccagga
     6781 ggcggcgcag cagttcttct cgcagcaggg ggtccagcag gggcaggcgc tgcgcgccaa
     6841 cctcgggggc ctgccggcgg tggccaacga gttcgcggcg aacacccagc aggggaccct
     6901 ggtcggcctg gccgccttcg tggaggaccg ggaccgggtc taccgcatcc tcggctacgc
     6961 cccgcagaac agctggcgtg gcaacgagtc ggccatctcc aacgcgctgg ccagcttcga
     7021 aagagtgacg gaccgccgtc tcctcaacgt cacgccgaag cggctggacg tggtgacggt
     7081 gccctcggcc atgagcctgg aggagttcaa ccggcgctat ccgtcgacgg tggacatcca
     7141 gactctggcc atcatcaacc aggcgaacgc cgatacccgg ttccccgcgg gaaccgaggt
     7201 caagagagtg gtgggcggcg aggtcccctc gtagctcacc attcacctcc acgggaaagg
     7261 gtgaggggcc cggagctcct caccctcttt ttcttcaggc cccccgcctt ctgtcgattt
     7321 ccggctcccc cgaacgacta cctggtgaat gaccagagcc gttcaaccat ccgatggagg
     7381 agccgatgaa gtacctgctg ctggcctacc atgacgagac aaagtgggac gcgctgtccg
     7441 atgacgaaaa ggacgccatc gggagccagt gcctgcccta cgacgaggag ctccggcagg
     7501 gcggtcacgt gatcgccatg ggaagcctcg agcacacgcg gatgagcacc gtgatgcgga
     7561 cccggagcgg ccggctgctc accaccgacg ggcccttcat cgagaccaag gagcagctcg
     7621 gcggcttcct catcatcgag gcgcgggacc tgaacgaggc cctccaggtg gccgcgaacc
     7681 atccggcggc gcggatgaac gagcgcctgg gctggtgcgt cgaggtgcgg ccgttcgagt
     7741 tcttcgagca gccctagcct ctggtaaagt gccccgccag cgaggagaca gtcatgtgca
     7801 ggaacatcaa gaccctgttc aacttcgacc ccccggcgac cgacgaggag gtccgggcgg
     7861 cgtcgctcca gttcgtccgc aagatctgcg gcttcacgag cccgtcgaag gcgaacgaag
     7921 ccgcgttcga cctcgccgtc gagcaggtcg ccgagtccgc ccgggccctg ctcgcctccc
     7981 tggtgaccag cgcgcccccg cacgaccgcg agatcgaggc ggcgaaggcg cgcgccaggt
     8041 cgctgcagag gttcggcggc gccgcgggag ccggcggatc ctgactccgg ccctcaccac
     8101 cctccagctc aaccgcgccc tgcttgcgcg gcagatgctg ctcgcccgcg aggacgcgac
     8161 cccactcctc gccgtcgagc ggctcgccgg cctgcaggcg cagatggccc ggccgccctt
     8221 cgtgggcctg tggacgcgcc tcgccggctt ccgccgggac ggcctcctcg ccccgctcca
     8281 ccggcgcgag atcgtccgcg tcacggcgat gcgcgccacg ctccacctcc tgaccgccgc
     8341 cgactacgtg gccctgcggg gcgccttcca gccggtgctc gccaaagcca tgcagtcgat
     8401 cctgcgcgcc cggtcggcgg aggtcgacct cgcccggatc gaggccgagg cgcgctcctt
     8461 cttcggcgag ggggcggcga ccttcgacgc cttccgcacc catctcaagg cgaagtaccc
     8521 ggagggggac gagcgcgcca tggcgtacgc gatccgcacg cacctgccgc tcgtccaggt
     8581 gccgggtgag caggcctggg gcttcccccc ggctcccgag ttcgcgctcg ccgagccctg
     8641 gctgaaggag ctgaaggagc tgaagaagca ggggaagaag atcccgatgg agcccgggcc
     8701 gccgcacgag ctggtccgcc gctacctcgc ggcattcggg cccgcgaccc ccggtgacgc
     8761 gcagacctgg tccggcctga gcggcctgcg cgaggtcttc gagtccctcc gtcccgagct
     8821 cgtcaccttc cgcgacgccc ggaagcgcga g
//
LOCUS       DASPJI010000494         5689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_327174, whole genome shotgun sequence.
ACCESSION   DASPJI010000494 DASPJI010000000
VERSION     DASPJI010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327174"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1743)
                     /locus_tag="VEL74_13485"
     CDS             complement(<1..1743)
                     /locus_tag="VEL74_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HYG63588.1"
                     /translation="MHRTLILACLASFTLASSAPAAAAPLMRSPALSRSEVAFVHAGD
                     LWVAGREGGQARRLTTGVGVETDPYFSPDGGQIAFTGEYEGNLDVYVVPASGGVPRRL
                     THHPGRDRAAGWTPDGKQVLFRSERSSYETFSRLYTVPPTGGFPADLPLPMAEQGSYS
                     ADGSRLAYVPQWNQSASATTYRSHKRYRGGLASPIWLARLADSSVEEIPRQDANDWNP
                     MWVGDRVYFLSDREGPATLYAYDTRSRQVTRVVDNPGGPDIMTAAAADGAIVYEQLGR
                     LHLLDLASGRATALDIRVQGDLPGVRPRFVDVSGEINKGALSPTGVRAVFEARGDILT
                     VPVGEGAVRNLTQTPGVAERDPAWSPDGRSIAYFSDESGEYALHVREQTGTGEVRKVG
                     LGEPPSFFYAPLWSPDSRKVVYTDKRLNVWWVDVAGKGRPVKIDTDTYDAPLRRLNPV
                     WAPDSRWIAYTKQLPNHMRAVFVYSLESGKARQVTDAMSDAMYAAFDKNGQYLYFTAS
                     TDVGPTTGWLDLSSVQRPVSRNVYAIVLDKTLPSPLAPRSDEEEVTEAAREDGDEEEG
                     EEDIGPVRVKVDFDG"
     gene            complement(1859..2248)
                     /locus_tag="VEL74_13490"
     CDS             complement(1859..2248)
                     /locus_tag="VEL74_13490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG63589.1"
                     /translation="MTEPRIPSRPRVLAVEDDRQTLQLLGLLLSSWGYEPILAEDGET
                     ALAALTPVGPLPEAVLLDLGLPGIDGCQVARELRRRPGGDAPLIVVVTGAHGEEARAR
                     EAGCDHYLTKPVNIPALRQVLERVRTG"
     gene            2322..4805
                     /locus_tag="VEL74_13495"
     CDS             2322..4805
                     /locus_tag="VEL74_13495"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYG63590.1"
                     /translation="MNRSRKPIAFVAALALLSSMSPAAGQSAGQGGGVTLEQIMADQD
                     WVGNPPENPYWADDGRAVFYQIKRRGSDIRDLYRLDLATGQSALIPPADLGKVDRPGE
                     ISRDLQWKVYAHEGDIFLKDLHNGTVRQITRTHDEESEPAFLVGDRRLSYRRGDTFFI
                     HDMETGLASQAAEIRLEKAPAERDEENGYLEKQQERLFDVIRKREGREKENREHRETL
                     QKADPNRPPLPWYLGTEVEIREASLSPTGEFLVLVTQPKAREEKAPVMPRYVTETGQV
                     ETRDVRVKVGAVPLPNQSLILLDLRSHQRHDLDTKVLPGIYDDPLKEIREKTEARRKA
                     QEEKEKAAQGGEKKPAEKAKDEKPKDEKQARALEVSALVWSQDGRHLAVQLLSQDNKD
                     RWIASLEPAKGPALTPRHRLTDPAWVIDYEFNDYGWLPGTSTLFYLSEESGYSQLYLD
                     DGKRKRQLSPKGERPYEVSAPVVTRDGRYIYYTANAAHPGQYETWRVETATAKAEQLT
                     RLGGEARAVLSPDESQLLLTFSSILRHYELYLQPAQPAADSRAARQLTQTHSAEWLAQ
                     DWIMPEVVAVPSSHQKDPIYSRVFVPKDFDPAKKYPAVVFTHGAGYLQNAHYGWSTYF
                     REFMFHTLLAQRGYVVLDIDYRGSQGYGRDWRTAIYRQMGYPEVEDMEDGVAWLVENR
                     SVDPARVGTYGGSYGGFLTFMAMFRKPEIFAAGAALRPVTDWAHYNHPYTANILNTPE
                     EDSEAYERSSPIEYAAGLAKPLLICTGVEDDNVLFQDSVRLVQRLIELEKQDFETAIY
                     PVEGHAFREPSSWLDEYRRILKLFETYLK"
     gene            complement(4798..5364)
                     /locus_tag="VEL74_13500"
     CDS             complement(4798..5364)
                     /locus_tag="VEL74_13500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG63591.1"
                     /translation="MDREAVRRRLGLTGTVLISVGHLTPRKGHHLAIEALCSLVGVTL
                     LIAGDGVYEQTLRELAATLEVADRVRFLGHVEQEGLKELYNAADALVLASSREGMANV
                     LLEAMACGTPVIATAVWGTPEAVSTPAAGVLMEERSSAALVAAARRLFDDYPDREDTR
                     QHAAGFRWERTSREHLEALHGALRLPST"
BASE COUNT          907 a         2031 c         1965 g          786 t
ORIGIN      
        1 gccgtcgaag tcgaccttca cccgcaccgg cccgatgtcc tcctctcctt cctcctcgtc
       61 gccgtcctcc cgcgccgcct ccgtcacctc ctcctcgtcg ctgcgcggag ccaggggcga
      121 ggggagggtc ttgtcgagga cgatggcata gacgttcctc gacaccgggc gctggacgct
      181 ggagaggtcg agccagccgg tggtggggcc gacgtcggtg ctggccgtga agtagaggta
      241 ctgtccgttc ttgtcgaagg cggcgtacat ggcgtcgctc atcgcgtcgg tgacctgccg
      301 cgccttgccg ctctccagcg aatagacgaa gaccgcccgc atgtggttcg ggagctgctt
      361 ggtgtaggcg atccagcggc tgtccggggc ccagacgggg ttgagccggc gcagcggcgc
      421 gtcgtaggtg tcggtgtcga tcttcaccgg tctgcctttg cctgccacgt ccacccacca
      481 gacgttgagc cgcttgtcgg tgtagacgac cttgcggctg tccggcgacc agaggggggc
      541 atagaagaag gacgggggct cgcccaggcc gaccttcctg acctcgccgg tgccggtctg
      601 ctcccgcacg tgcagggcgt actcgccgga ctcgtcggag aagtaggcga tggagcggcc
      661 gtcgggggac caggccgggt cccgctcggc gacgcctggc gtctgggtga ggttgcgcac
      721 cgccccctcc cccaccggca cggtcaggat gtcgccgcgc gcctcgaaca ccgcgcgcac
      781 gccggtgggc gacagcgccc ccttgttgat ctcgccggag acgtcgacga agcgcgggcg
      841 caccccgggg aggtccccct gcacccggat gtccagcgcc gtggcccggc ccgaggccag
      901 gtcgaggagg tggaggcggc cgagctgctc gtagacgatg gcgccgtcgg ccgccgcggc
      961 cgtcatgatg tccgggccgc ccgggttgtc caccacccgg gtcacttggc gggaccgggt
     1021 gtcataggcg tagagggtgg ccggcccctc gcggtcggag aggaagtaga cgcggtcgcc
     1081 gacccacatc gggttccagt cgttggcgtc ctgccggggg atctcctcga cgctcgagtc
     1141 ggcgagccgg gcgagccaga taggggaggc gagcccgccc cggtagcgct tgtgcgaacg
     1201 ataggtggtc gccgacgccg actggttcca ctgcggcaca taggccagac gggagccgtc
     1261 ggcggagtag gagccctgct cggccatggg cagggggagg tcggcgggga agccgcccgt
     1321 cgggggcacg gtgtagagcc ggctgaaggt ttcgtagctg ctccgctccg accggaagag
     1381 cacctgcttg ccgtccgggg tccagcccgc ggcccggtcg cggccggggt gatgggtcag
     1441 gcgccgcggg acgccgccgg acgccggtac cacgtagacg tcgaggttcc cctcgtactc
     1501 gccggtgaag gcgatctgcc cgccgtccgg agagaagtag gggtcggtct ccacaccgac
     1561 gccggtggtg aggcgccgcg cctgcccccc ctcgcggccg gccacccaga ggtcaccggc
     1621 gtggacgaag gcgacctcgg agcggctgag ggccggggag cgcatcaggg gcgccgccgc
     1681 cgccggggcg gaggaggcga gggtaaagga agcgagacag gccaggatca gagttcggtg
     1741 catgatcagc atgaatctcc ctcgagaagg tccagggacg gcaaggatag cgccggacgg
     1801 ggctccgagc ggagcccggc acctgtgtac gcaggacagg agcggcggtt tcagccactc
     1861 acccggtccg tacccgctcc aggacctgcc gcagggccgg gatgttcacc ggcttggtga
     1921 ggtagtggtc gcagcccgcc tcgcgggcac gggcctcctc cccgtgggcg ccggtgacga
     1981 ccacgatcag gggggcgtcc ccgccgggcc ggcggcgcag ctcgcgggcc acctggcagc
     2041 cgtcgatgcc cggcaggccc aggtcgagga ggacggcctc cggcagcggt cccaccgggg
     2101 tcagcgcggc cagcgcggtc tcgccgtcct ccgccaggat gggctcataa ccccaggagg
     2161 acagcagaag ccccaggagc tggagggtct ggcggtcgtc ctccaccgcc agcacccgcg
     2221 gacgggacgg tattcggggc tcggtcatgg ttctctctcg gctgtcggca tcttatgacg
     2281 gactgccctc ggggcgggtg gcggtgttat cttcacgccc catgaaccgg tcccgcaaac
     2341 ccatcgcctt cgtcgccgcc ctcgcgctcc tctcgtccat gagcccagcc gccggacagt
     2401 ccgccggaca ggggggcgga gtcacgctgg aacagatcat ggccgaccag gactgggtcg
     2461 ggaatccgcc cgagaacccc tactgggccg acgacggccg ggcggtcttc taccagatca
     2521 agcgccgggg atcggacatc cgcgacctct accggctgga cctcgccacc ggccagtccg
     2581 ccctcatccc ccccgccgac ctcggcaagg tggaccggcc gggcgagatc agccgcgacc
     2641 tccaatggaa ggtatacgcg cacgaaggcg acatcttcct caaggatcta cacaacggca
     2701 ccgtccgcca gatcacccgc acccatgatg aggagagcga gcccgccttc ctggtgggcg
     2761 atcgccgcct ctcctaccgg cgcggcgaca ccttcttcat ccatgacatg gagacggggc
     2821 tcgcctccca ggccgccgag atccgcctgg agaaggcccc ggccgagcgg gacgaggaga
     2881 acggctacct cgagaagcag caggagcggc tcttcgacgt gatccgcaag cgcgagggcc
     2941 gggagaagga gaaccgcgag caccgggaga ccctgcagaa ggccgacccg aaccgcccgc
     3001 cgctcccctg gtacctcggc accgaggtcg agatccgcga ggcctccctc tctcccaccg
     3061 gcgagttcct cgtcctggtc acccagccca aggcccggga ggagaaggcg ccggtcatgc
     3121 cgcgctacgt caccgagacc ggccaggtgg agacccgcga cgtccgcgtc aaggtgggcg
     3181 ccgtcccgct ccccaaccag agcctgatcc tcctcgacct gcgctcccac cagcggcacg
     3241 acctcgacac caaggtcctc cccggtatct acgacgaccc gctgaaggag atccgcgaga
     3301 agaccgaggc gcgccgcaag gcccaggaag agaaggagaa ggcggcccag ggcggcgaga
     3361 agaagccggc ggagaaggcc aaggacgaga agccgaagga cgagaagcag gcgcgggcgc
     3421 tggaagtctc ggcgctcgtc tggagccagg acggccgcca cctggccgtg cagctcctct
     3481 cccaggacaa caaggaccgc tggatcgcct cgctggagcc ggccaagggc cccgccctga
     3541 ccccgcgcca ccggctgacc gacccggcct gggtcatcga ctacgagttc aacgactacg
     3601 gctggctgcc cggcacctcc accctcttct atctgtccga ggagagcggc tactcccagc
     3661 tctatctgga tgacggaaag cgcaagcgac agctcagccc caagggggag cgcccctacg
     3721 aagtatcggc gccggtggtc acccgcgacg gccgctacat ctactacacg gcgaacgccg
     3781 cgcaccctgg ccagtacgag acctggcggg tggagacggc tacggccaag gccgagcagc
     3841 tcacccggct gggcggggag gccagggcgg tgctctcgcc cgacgagagc cagctcctgc
     3901 tcaccttctc gagcatcctg cggcactacg agctctacct ccagccggcg cagcccgctg
     3961 ccgactcccg ggcggcgcgc cagctcaccc agacccacag cgcggagtgg ctggcccagg
     4021 actggatcat gcccgaggtg gtggccgtcc cctcctccca ccagaaggac cccatctact
     4081 cgcgggtctt cgtgccgaag gacttcgacc cggccaagaa gtatccggcg gtcgtcttca
     4141 cccacggcgc cggctacctg cagaacgccc actacggctg gtccacctac ttccgcgagt
     4201 tcatgttcca caccctgctc gcccagcgcg gctacgtcgt gctcgacatc gactatcgcg
     4261 gctcacaggg ctacggccgc gattggcgca ccgccatcta ccggcagatg ggctaccccg
     4321 aggtcgagga catggaggac ggcgtcgcct ggctggtcga gaaccgcagc gtcgacccgg
     4381 cgcgggtggg gacctatggc ggctcctacg gcggcttcct caccttcatg gccatgttcc
     4441 gcaagccgga gatcttcgcc gccggcgcgg cgttgcggcc ggtgacggac tgggcccact
     4501 acaaccaccc ctacacggcg aacatcctca acaccccgga ggaagactcc gaggcctacg
     4561 agcgcagctc gcccatcgaa tacgcggcgg ggctcgccaa gccgctgctg atctgcaccg
     4621 gcgtcgagga cgacaacgtg ctgttccagg actcggtgcg cctggtgcag cgcctgatcg
     4681 agctggagaa gcaggacttc gagaccgcca tctacccggt ggagggccac gccttccgcg
     4741 agccgtcgag ctggcttgac gagtaccggc ggatcttgaa gctgttcgag acctacctca
     4801 agtagagggg agtcgcagtg cgccgtggag cgcctccagg tgctcccggc tcgtccgctc
     4861 ccagcggaag ccggcggcgt gctggcgagt gtcctcccgg tccggatagt cgtcgaacag
     4921 ccggcgcgcc gccgccacca gcgcggcgga gctgcgctcc tccatcagca cgccggccgc
     4981 cggcgtgctg acagcctcgg gggtgcccca gaccgcggtg gcgatcaccg gcgtgccgca
     5041 ggccatggcc tccagcagga cgttggccat cccctcgcgg ctcgacgcca gcaccagggc
     5101 gtcggcggcg ttgtagagct ccttgagccc ctcctgctcg acgtggccga ggaagcggac
     5161 gcggtccgcc acctccaggg tcgccgccag ctcccgcagc gtctgctcgt agacgccgtc
     5221 gcccgcgatg agcagtgtca cgccgaccag actgcagagc gcctcgatgg ccaggtgatg
     5281 ccccttgcgc ggcgtcagat gccccactga aatgaggacc gtgccggtga ggcccagccg
     5341 ccgccggacc gcctcccggt ccaccggatg gaagagctcc ggggatcacg cggatcttgt
     5401 cggcggatgc cccccaggcc accagcttgg ccttgagcgc ctggcagacc gagctgacgg
     5461 aggccgcctg ctccactgcc cagagcagca gccggcgggg gacggggtac cgcggatagg
     5521 tgatcacgtc atcgcccagc atgctcatga tcagcggctt gccgaactgc cgggccagaa
     5581 tcgccgccgc cgcgccgtcc ggatagacgt agtgggcgtc gatgacgtcg aagtcgaagc
     5641 cctcgtccag gagctcctcc agggccttcc gtgccgccgc cgccatcag
//
LOCUS       DASPJI010000495         3259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_90344, whole genome shotgun sequence.
ACCESSION   DASPJI010000495 DASPJI010000000
VERSION     DASPJI010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90344"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..2905
                     /gene="uvrA"
                     /locus_tag="VEL74_13505"
     CDS             65..2905
                     /gene="uvrA"
                     /locus_tag="VEL74_13505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HYG63592.1"
                     /translation="MSKTSAILVERARTHNLKGVDCRVPHRAVTVVTGPSGAGKSSLA
                     FDTIFAEGQRRFVESMSTYARQFLDQMERPPVDAIHNILPAVALEAKNAVKNARSTVG
                     TITEIHDVLRLLYTHLGEVACPRGHGPVRPYSPEDAAADLIARGEPFTIVVRLPRPAR
                     GADQALGELIRQGFQRRLEEGKDGGEEVARMEPGEKWPAKRDPLPLVLGRFTPKPDAV
                     GRIVDTLEQAYRMSGGRVEARTASGVRYYSREISCPVCGETVRRPTPPLFSFNSPLGA
                     CPTCQGFGRVIGIDRERVVPDPRKTLAERPIAPWNTPAHEELYDGLLAAARKRKVPLD
                     VPWSALAPEDREWIWSGEGKFTNLEEFFHWLEGRTYKVHVRVLLARYRAYNPCPDCDG
                     ARLQPEARVVTVEGMTLPQLTALSVEQLRAWLRARRWTPRQREVAGHLLDELTERVEV
                     LHRVGLDYLTLDRQARTLSGGETQRIHLAAALGSGLTSTLYVLDEPTIGLHPQDSERL
                     LSLLRDLAARGNTVLVVEHDRTLIRGADHVIDMGPAAGERGGEVVIEGPIEAILASEE
                     SLTGRYLRERPVTSARRHVARFRREQGWETLSDELSALPRLSVRNARAHNLKGIDVEL
                     PLGAMVAVTGVSGSGKSTLIENVVYGTYQRSRGVVDADPGECDALIGLDGLADVTLVD
                     QRPLGRSSRSNPITYIKAYDELRKLFAATSDAKARRITPAHFSFNIDRGRCPTCEGTG
                     VTEVDMQFMAPVTVPCETCQGHRFQPEVLAVRWRGRNISEVLDLTVDSALEFFSDQKG
                     LCRRLRVLADVGLGYLRLGQPTSTLSGGEAQRLKLASFLDRPAAEGKRLFLFDEPTTG
                     LHLADIDLLYHTLRRLVQRGDGVVVVEHSSDLIARADWIVDLGPGGGVHGGEVLYSGP
                     LEPYLDRVDSPTAEELRRHLKWPEEAAVAAAR"
     gene            2993..>3259
                     /locus_tag="VEL74_13510"
     CDS             2993..>3259
                     /locus_tag="VEL74_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63593.1"
                     /translation="MIHEETSPLRSLETAGDAPVRPVAAAAGRPRLWWVGTVGALFLG
                     GVLLVAVWAKMLDPLAFAEQIRQEKLDFLLSAEAVALIALALEAG"
BASE COUNT          441 a         1240 c         1124 g          454 t
ORIGIN      
        1 ctgcttcgga acgtcccggg ctctgccccg tgggccccgg gtctgcggcg gtgcagcaac
       61 cccgatgtcc aagacgtcag ccatcctggt cgagcgggcc cggacccaca acctcaaagg
      121 tgtggactgc cgggtgccgc accgcgccgt gaccgtggtc accggcccct cgggcgccgg
      181 caagtcgtcc ctcgccttcg acaccatctt cgccgagggg cagcgccggt tcgtggagtc
      241 gatgtccacc tatgcccggc agttcctcga ccagatggag cggccgccgg tcgacgcgat
      301 ccacaacatc ctgcccgccg tcgccctgga ggcgaagaac gcggtcaaga acgcccgctc
      361 caccgtcggc accatcaccg agatccacga cgtcctgcgc ctcctctata cccatctggg
      421 cgaggtggca tgcccccgcg gccacggccc ggtgcgcccc tactcccccg aggacgccgc
      481 cgcggacctg atcgcccggg gggagccgtt taccatcgtg gtccgcctcc cccgtccggc
      541 ccgcggcgcc gaccaggccc tgggcgagct gatccgccag gggttccagc gccggctcga
      601 ggaaggaaag gacggcggcg aggaggtggc ccgcatggag ccgggcgaga agtggccggc
      661 gaagcgcgac cccctccccc tggtgctcgg ccgcttcacc cccaagccgg acgccgtggg
      721 ccgcatcgtc gacaccctcg agcaggccta ccggatgagc ggcggccggg tggaggcacg
      781 gacggcctcc ggcgtccgct actacagccg cgagatctcg tgcccggtgt gcggcgagac
      841 cgtgcgccgg ccgacgcccc ccctgttctc cttcaactcg ccgctcggcg cctgccccac
      901 ctgccagggg ttcggccggg tcatcggcat cgaccgcgag cgggtcgtcc ccgatccccg
      961 gaagacgctc gccgagcgcc cgatcgcgcc gtggaacacg ccggcccacg aggagctgta
     1021 cgacggcctc ctcgccgccg ctcgcaagcg gaaggtcccc ctggacgtcc cctggagcgc
     1081 gctcgccccc gaggaccggg agtggatctg gagcggggag gggaaattca ccaacctgga
     1141 ggagttcttc cactggctgg aggggcggac ctataaagtc cacgtgcgcg tgctcctcgc
     1201 ccgctaccgc gcctacaacc cctgcccgga ctgcgacggc gcccgcctcc agcccgaggc
     1261 gcgggtggtc accgtcgagg ggatgaccct gccgcagctc acggcgctga gcgtcgagca
     1321 gctccgggcc tggctgcgcg cccgccgctg gacgccgcgg cagcgcgagg tcgccggcca
     1381 cctcctcgac gagctcaccg agcgggtgga ggtgctccac cgcgtcggcc tcgactacct
     1441 caccctcgac cgccaggcgc ggacgctgtc cggcggcgag acgcagcgca tccacctggc
     1501 cgcggccctg ggctcggggc tcaccagcac cctctacgtc ctcgacgagc cgaccatcgg
     1561 cctccacccg caggacagtg agcgcctgct gtcgctgctg cgcgacctcg ccgcccgcgg
     1621 caacaccgtg ctggtggtcg agcacgaccg gacgctcatc cgcggcgccg accacgtcat
     1681 cgacatgggc ccggcggccg gcgagcgcgg cggcgaggtg gtgatcgagg ggccgatcga
     1741 ggccatcctg gcgtccgagg agtcgctgac cggccgctat ctcagagagc gccccgtgac
     1801 ctcggcgcgc cggcacgtcg cccgcttccg ccgcgagcag ggatgggaga cgctgagcga
     1861 cgagctgtcc gccctccccc gcctctcggt gcgcaacgcc cgcgctcaca acctcaaggg
     1921 gatcgacgtc gagctccccc tgggagcgat ggtggcggtc accggcgtct cgggctcggg
     1981 caagtcgacg ctgatcgaga acgtcgtcta cggcacctac cagcgctcgc gcggcgtggt
     2041 ggacgccgac ccgggggagt gcgacgcctt gatcgggctc gacgggctcg ccgacgtcac
     2101 cctggtggac cagcgcccct tgggccggtc gagccgctcc aacccgatca cctatatcaa
     2161 ggcctacgac gagctgcgca agctgttcgc cgccacctcg gacgccaagg cgcggcgcat
     2221 cacaccggcc cacttctcgt tcaacatcga ccgcgggcgc tgccccacct gcgaggggac
     2281 gggggtgacc gaggtcgaca tgcagttcat ggcgccggtg accgtgccct gcgagacctg
     2341 ccaggggcac cgcttccagc ccgaggtgct ggcggtgcgc tggcgcggcc gcaacatctc
     2401 cgaggtgctg gacctgaccg tcgactcggc gctggagttc ttctccgacc agaaggggct
     2461 ctgccgccgg ctgcgggtgc tggccgacgt cggcctgggc tatctgcggc tcggccagcc
     2521 gacctcgacg ctgtcgggcg gcgaggcgca gcggctgaag ctcgccagct tcctcgaccg
     2581 tccggccgcc gaggggaagc gcctcttcct cttcgacgag ccgaccaccg gtctgcacct
     2641 ggcggacatc gacctcctct accataccct gcggcgcctg gtgcagcgcg gcgacggcgt
     2701 ggtggtggtc gagcacagct ccgacctcat cgcccgcgcc gactggatcg tcgacctggg
     2761 gcccggcggc ggcgtccacg gcggcgaggt cctctactcc ggccccctgg agccgtacct
     2821 cgaccgggtg gacagcccta cggccgagga gctgcggcgt catctgaagt ggcccgagga
     2881 ggccgccgtg gcggcggccc gctgacctgc ggggaaggcc cgcaattttt cggcgcctcc
     2941 cgccgtttct ccagttgcag cgcccgaacc gcgccattag gatggatatt tgatgatcca
     3001 cgaagagacc tcgcccctcc gttccctcga gaccgccggc gacgccccgg tccgcccggt
     3061 ggccgccgcc gcgggacgcc cgcgcctctg gtgggtgggg accgtgggcg cgctcttcct
     3121 gggcggggtg ctgctggtgg cggtctgggc caagatgctc gatccgctgg ccttcgccga
     3181 gcagatccgg caggagaagc tggacttcct gctctcggcc gaggccgtgg cgctgatcgc
     3241 cctggccctg gaggcgggg
//
LOCUS       DASPJI010000496         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_29886, whole genome shotgun sequence.
ACCESSION   DASPJI010000496 DASPJI010000000
VERSION     DASPJI010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29886"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1879)
                     /locus_tag="VEL74_13515"
     CDS             complement(<1..1879)
                     /locus_tag="VEL74_13515"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYG63594.1"
                     /translation="MEESRFRSLAESERFGVVVADFAGNLLDFNDAFLRLAGYGREEL
                     RRGTVGWRELTPPEYWRRDEMAIEEMRRTGACSPYEKELVNPDGRRVPVLLTGSLVDE
                     KAGTCVDIVVDLSGRKRAESALQESRERLQFALDAARMVAWDWDVETGRMHWSANAQD
                     VLGLPPEPTLETALAMVHPDDRARVDRLVERALQGEVEYAAEYRLVRPSGVIWVYDRG
                     AVIRNIQGRPVRMTGVCQDVTERRRAEEENSLLLARERAARARAEAAEQRAAFLAEAG
                     EVLASSLDDRTTLRTLARLAVPRVADYCVLLEVGAGNRITPVAFEHVDPGKETFLERV
                     GELYRVRSDDPRSLVGAILGTGRSEIVGYPTLVEWEQEIPEDPELREIFGELHPVSGM
                     LLPLVVRGEVVGILVLALSESGRRFDDADLELGVELARRAALAIDNARLYQEARAANT
                     AKDRFLATLSHELRTPLAPVLASVSALEKEERIEPRLRDVLSMVRRNVELEARLIDDL
                     LDVTRAVHGKMTLSRDVCDLHRVLGQALETCCSPVLRSQRQVSTRLGAERHKLRGDAR
                     RLTQIFLNLLQNAIKFTPPGGSITVRSLNEDALDGSWLVVEVEDSGMGIEPDLLPRMF
                     EP"
     gene            2054..>3193
                     /locus_tag="VEL74_13520"
     CDS             2054..>3193
                     /locus_tag="VEL74_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYG63595.1"
                     /translation="MNRVSCLLTLAGVAVLVVMSFVAGVSYANRQEPGEVSAPPAGDT
                     AAQPGLLPAPARRELLEEERSTIELFERASPSAVFVTSAALQRDFFSMNLTEIPQGSG
                     SGFVWDREGHVVTNFHVIAQGNAFQVILSDSSAWEARVVGYAPEKDLAVLRVEVPPDR
                     LRPLPVGSSEDLEVGQKVFAIGNPFGFDQSLTTGIISALGREIQSLAGVPIRDVIQTD
                     AAINPGNSGGPLLDSAGRLIGVNTAIYSPSGAYAGIGFAIPAHAVAWAVPDLIRYGRI
                     QRPTLGVEVASDSLERRAGIEEGALVVRVEEGSAADQAGLQGLRRDALGRWVVGDIIL
                     RVDGEEVDSAGDLLLALEERKAGDSVPVEVLRDGRRQRLDVTLAAP"
BASE COUNT          474 a         1132 c         1112 g          475 t
ORIGIN      
        1 acggctcgaa catgcggggc agcaggtccg gctcgatgcc catgccagag tcctccacct
       61 cgaccaccag ccaggagccg tccagcgcgt cctcgttcag cgaccgtacg gtgatggagc
      121 cgccgggcgg cgtgaacttg atcgcgttct ggagcagatt gaggaagatc tgggtgagac
      181 gccgggcatc gccgcgcagc ttgtggcgct ccgccccgag ccgggtgctg acctggcgtt
      241 gcgacctgag cacgggcgag caacaggtct ccagcgcctg cccgaggacg cggtgcaggt
      301 cgcagacgtc gcgggagagg gtcatcttgc catggacggc gcgggtgacg tccagcaggt
      361 cgtcgatgag ccgcgcctcc agctccacgt tgcggcggac catggagagg acgtcgcgca
      421 gccggggctc gatccgctcc tccttctcca gggcggagac cgaggcgagg accggcgcga
      481 gcggtgtgcg gagctcgtgg ctgagcgtgg cgaggaagcg gtctttggcg gtgttggccg
      541 cccgtgcctc ctgatagagg cgggcgttgt cgatggccag agcggcccgg cgggccagct
      601 ccacccccag ctccaggtcg gcgtcgtcga accggcggcc cgactccgac agcgccagga
      661 ccaggatgcc gacgacctcg ccccggacca ccagggggag gagcatcccg gagaccgggt
      721 ggagctcgcc gaagatctcc cgcagctccg ggtcctcggg gatctcctgc tcccactcga
      781 ccagggtggg atagccgacg atctcggacc gtccggtgcc caggatcgcg ccgaccaggc
      841 tccgcggatc gtccgagcgg acgcggtaga gctcgccgac ccgctcgagg aacgtctcct
      901 tgccgggatc cacgtgctcg aaagcgaccg gggtgatgcg gttccccgcg ccgacctcca
      961 gcagcacaca gtagtcggcg acccggggga cggcgaggcg ggcgagcgtc cggagggtgg
     1021 tgcggtcgtc gagcgacgag gcgagaacct cgccggcctc ggcgaggaag gcggcccgct
     1081 gctccgccgc ctcggcgcgc gcccgggcgg cccgctcgcg cgccagcagc agggagttct
     1141 cctcctccgc ccgccgtctc tcggtgacgt cctggcagac gccggtcatc cgcaccggcc
     1201 gcccctggat attgcggatc accgcgccgc ggtcgtaaac ccagatgacg ccgctgggcc
     1261 gtaccagacg gtactcggcc gcgtactcca cctcgccctg caaggcgcgc tcgaccagcc
     1321 ggtccaccct cgcccggtcg tccggatgga ccatggccag cgcggtctcg agcgtcggct
     1381 cgggaggcag gccgaggacg tcctgcgcgt tggccgacca gtgcatccgg ccggtctcca
     1441 cgtcccaatc ccaggccacc atgcgcgcgg cgtccagggc gaactggagg cgctcccgac
     1501 tctcctgcag agccgactcg gcacgcttgc gaccggaaag atccaccacg atatcgacac
     1561 aggtgccggc cttctcgtcc accagggagc cggtcagcag gaccggtaca cgccggccat
     1621 cggggttgac cagctccttt tcgtacggtg agcaggcacc cgtccgccgc atctcctcga
     1681 tcgccatctc atcgcggcgc cagtactccg gcggcgtcag ctcgcgccag cccacggtac
     1741 cgcggcgcag ctcctccctg ccgtagccgg cgagccgcag gaaggcatcg ttgaaatcca
     1801 ggaggttgcc ggcaaagtcg gcgaccacca cgccgaaccg ttcggattcg gcaagagacc
     1861 ggaaccggga ctcttccata caggctcctc gcttctggga ccaatacccg cgctgagaag
     1921 ccatgagact ccagccgcgg gacggcccgt acccggtcgg gcgttttgtc aatagtatat
     1981 aacgaatttc cagaagaagt tgctccaggt gccctgctat gatttttcgc tacggcgaga
     2041 gggggtgtcc cggatgaatc gagtgagctg tctcttgacg ctggccggcg tggcggtgct
     2101 ggtcgtgatg agcttcgtgg ccggtgtctc ctacgccaat cgtcaggagc cgggcgaggt
     2161 gtcagcccct cccgcgggcg ataccgcggc gcagccgggc ctgctgccgg cgccggcacg
     2221 gcgcgagctg ctggaggagg agcggtccac catcgagctg ttcgagcgcg cctccccgtc
     2281 ggccgtgttc gtcacctcgg ccgccctcca gcgggacttc ttcagcatga atctcaccga
     2341 gatcccgcaa gggagcggct cggggttcgt ctgggaccgc gaggggcacg tggtcaccaa
     2401 cttccacgtc atcgcccagg gaaacgcctt ccaggtcatc ctctccgaca gctccgcctg
     2461 ggaggcccga gtggtgggct atgccccgga gaaggatctg gccgtgctgc gcgtcgaggt
     2521 gccgcccgac cgcctccggc cgctgccggt cggcagctcc gaggatctcg aggtgggcca
     2581 gaaggtcttc gccatcggca acccgttcgg cttcgaccag tcgctgacca ccggcatcat
     2641 cagcgcgctc gggcgggaga tccagtcgct cgccggcgtc cccatccgtg acgtcatcca
     2701 gaccgacgcg gctatcaatc ccggcaactc gggggggccg ctgctcgaca gcgccggccg
     2761 cctcatcggc gtcaacaccg cgatctacag cccgtccggt gcctacgccg gcatcggctt
     2821 cgccatcccc gcccacgccg tggcgtgggc ggtgcccgac ctcatccgct acgggcggat
     2881 ccagcgcccc actctggggg tcgaggtggc ctccgactcc ctggagcgcc gggccgggat
     2941 cgaggagggg gctctggtcg tgcgcgtcga ggaggggagc gccgccgacc aagcgggtct
     3001 ccaggggctg cgtcgcgacg cactgggccg ctgggtggtg ggcgacatca tcctgcgcgt
     3061 cgacggcgag gaggtcgact ccgcggggga cctcctcctc gctctcgagg agaggaaggc
     3121 cggagactcg gtgcccgtgg aggtcctgcg ggatggccgg cgccagcggc tcgacgtcac
     3181 cctggccgcc ccc
//
LOCUS       DASPJI010000497         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_200922, whole genome shotgun sequence.
ACCESSION   DASPJI010000497 DASPJI010000000
VERSION     DASPJI010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200922"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /locus_tag="VEL74_13525"
     CDS             complement(<1..1732)
                     /locus_tag="VEL74_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63596.1"
                     /translation="MTKRWKWARRLLIAVLLVAALYPLTANVFLNTPLGSWVVNRRPS
                     VQVTWDSAWSLWPGRVHARGLEVRGHQRSVRWWVAAERADGYIDLPGLFQRRIEIRDL
                     RGEGVRSSVIREPRKPGALRPRRRPGRKGWTTRLPGVTLATVREIRYGDFVLSGSGEA
                     RGGFSVQVGGDFQLLPTTLRMTGARLDRGPGEARQLLVRDADVRTEVRIEPYAVREHP
                     GMEGWDFLSGHLEARGSVPDPPLLDRLGNAAGGAASLAVDVRAGRGRFVPGSRVSIEP
                     RPGREQAPLRVAGSVAAGPAGPVLLLTLQAAGLQLGATTDRPPLIHADALDLAAKSSE
                     VRLRHLFTTVRQINRSRTMSSPLTGTARARGVRLDLRGSRLASRIDLDQAEGRVDLAA
                     LLERRLILTGLRSEGGAIRLALAGPSGTRPSAAPPWAVHLDGARLAGLREIGFEPFVL
                     RGDTALEASLSLEPGGPLQVDTAALSIRGASLVAEGATVAQRVGIQARGRLDPMVLGE
                     LPGIQALRFVSGSVDLTGRISSFGFLETYFAGTPWLNFSGSGDLRLGLRVERGHLGPG
                     TTIAIPNARLE"
     gene            1869..>2553
                     /locus_tag="VEL74_13530"
     CDS             1869..>2553
                     /locus_tag="VEL74_13530"
                     /inference="COORDINATES: protein motif:HMM:NF025244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 family protein"
                     /protein_id="HYG63597.1"
                     /translation="MPSPSLEVVFRRRGTPLGAAEELRPLAVEFPTVQIGPSRSPFGE
                     EEIPFCTLEAVEWEHPTFGFYEFDARMNDLAERTDGHPFGLRLLGPAERLPSLAFEVL
                     TRCQRLADRRNHESCGPEFDRVLRCHRELHNLAKPLVRADHSHTLDTWQWVLRLNRGA
                     GLAVQLAALFHDIERLVSEADVRVEHQAPIYQSFKDDHARRGAAMAVEALAEAGIDEP
                     ACRRVARLIG"
BASE COUNT          349 a          968 c          908 g          328 t
ORIGIN      
        1 cctccaaccg ggcgttgggg atcgcgatag tggtccccgg gccgaggtga ccgcgctcga
       61 cccgcagccc cagcctgaga tcgccgctgc cgctgaagtt cagccagggg gtgcccgcga
      121 agtaggtctc gaggaaaccg aaggaggaga tccggccggt gaggtccacg gagcccgaga
      181 cgaagcggag cgcctggatg cccggcagct ccccgagaac catgggatcg agccgtccgc
      241 gggcctggat gcccactctc tgggccaccg tcgcgccctc cgctacgagg ctcgcacccc
      301 ggatggacaa ggcggcggtg tccacctgca gcggtccgcc gggctcgaga gacaggctcg
      361 cctccagcgc ggtgtctccc cgcaggacga acggctcgaa gccgatctcg cgcaggccgg
      421 cgagccgcgc cccgtccaga tggaccgccc acggcggcgc cgcgctcggc cgcgtcccgg
      481 aggggcccgc cagggcgagg cggatggccc ccccttccga gcgcaggccg gtgaggatca
      541 gccgccgctc cagcagcgcc gccaggtcga ccctgccctc ggcctggtcg aggtcgatgc
      601 ggctggcgag gcggctcccc cgcaggtcga ggcgcacgcc gcgggcccgc gcggtcccgg
      661 tcagagggga cgacatcgtc cgggagcggt tgatctgccg gacggtggtg aacagatggc
      721 ggagccgcac ctcggagctc ttcgccgcca ggtcgagggc gtcggcgtgg atgagagggg
      781 gccggtccgt ggtcgcaccc agctgcagcc cggcggcctg gagcgtcagg aggaggaccg
      841 ggcccgccgg ccccgcggcc accgagccgg ccacccgcag cggcgcctgc tcgcggccgg
      901 gccggggctc gatggacacc cggctccccg gcacgaaccg gccgcgcccg gcccggacgt
      961 ccaccgccag gctcgccgca cccccggcgg cgttgcccag gcggtccagc agcggcgggt
     1021 cgggcacgct cccccgggct tccaggtggc cggagaggaa gtcccacccc tccatccccg
     1081 ggtgctcgcg caccgcgtag ggctcgatcc gcacctcggt ccggacgtcg gcgtcccgca
     1141 ccaggagctg ccgcgcctcg cccggccccc ggtcgaggcg cgcgccggtc atcctcagcg
     1201 tcgtgggcag gagctggaag tcgccgccga cctggacgga gaagcccccg cgggcctcgc
     1261 ccgaaccgga gaggacgaaa tcgccatacc ggatctcgcg cacggtggcg agggtgacgc
     1321 cgggcagccg ggtggtccac cccttccggc ccgggcggcg gcgagggcgg agggctccgg
     1381 gcttgcgcgg ctcccggatc accgacgagc gcaccccctc cccccgcagg tcccgaatct
     1441 cgatgcggcg ctggaagagc ccgggcaggt cgatgtagcc gtcggcgcgc tcggccgcga
     1501 cccaccaccg cacgctgcgc tggtgccccc gcacctccag gccccgcgcg tgcacccggc
     1561 cgggccagag gctccacgcc gagtcccagg tcacctgcac gctcggccgg cggttgacca
     1621 cccaggaccc cagcggggtg ttgaggaaca cgttggccgt cagcgggtac agggccgcca
     1681 cgaggaggac ggcgatgagc agccgccggg cccatttcca tcttttcgtc atcggctccc
     1741 ctctacgcaa gggttgggcc tgacactgga accggctggc gggtcgcgtc ttcccgagtc
     1801 cttccagccc ctgctctggc tcgcgttttg catttagtaa accatgaatg aagccactag
     1861 gaggagccat gccctcgccg tccctggaag tcgtctttcg cagaaggggt acgcctctcg
     1921 gggcggccga ggagctgcgc cctctggccg tggagttccc cacggtccag atcgggccct
     1981 cccgctctcc gttcggcgag gaggagatcc ctttttgcac acttgaagcc gtggagtggg
     2041 agcatcctac cttcggcttt tacgagttcg acgcccgcat gaacgatctg gcggagcgga
     2101 cggatggcca ccccttcggg ctgcggctcc tggggccggc ggagcggctg ccctcgctgg
     2161 ccttcgaggt cctgacccgc tgccagcggc tggcggaccg gcggaaccac gagtcgtgcg
     2221 gccccgagtt cgaccgcgtc ctccgctgcc accgcgagct ccacaacctc gccaagccgc
     2281 tggtgcgcgc cgaccactcc cacaccctgg acacctggca gtgggtgctg cggctcaacc
     2341 gcggcgccgg cctggcggtc cagctcgccg cgctgttcca tgacatcgag cgcctggtct
     2401 ccgaggccga tgtccgcgtc gagcaccaag ccccgatcta ccagagcttc aaggacgacc
     2461 acgcccggcg cggcgccgcc atggcggtgg aggccctcgc cgaggcgggc atcgacgaac
     2521 cggcctgccg gcgcgtcgcc cggctgatcg gcg
//
LOCUS       DASPJI010000498         6533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_327340, whole genome shotgun sequence.
ACCESSION   DASPJI010000498 DASPJI010000000
VERSION     DASPJI010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327340"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VEL74_13535"
     CDS             complement(<1..203)
                     /locus_tag="VEL74_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63598.1"
                     /translation="MHRQDEKVVVAGREGVRSDPARLLRVRPPSPSPSASSRLRRADR
                     TPSLRGPEEQTPEERPPGEIERTG"
     gene            198..>6533
                     /locus_tag="VEL74_13540"
     CDS             198..>6533
                     /locus_tag="VEL74_13540"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63599.1"
                     /translation="MHHIVSDAWSMDVLARELGLLYASEPPPPLPIQYADFALWQRRH
                     LSSELLADHLTWWRHRLAGAPPLLDLPTDHPRPAVRTQRGDELSFHLPEHLSHTLESL
                     SHQEGATLFMTLLAAFQVLLARLANQQDVSVGTPIAGRRQAETEPLIGFFVNTLVMRT
                     DLSGEPVFRTLLGRVRDMALGTYAHQDLPFERLVEELAPERNLAHTPLFQAVFTLQRP
                     PAGLGTLPGLEVSPVRLGARTAKFDLTLTLIYEPGGLDGWMEFSADLFDRTSVQRWAG
                     HFQALLEGVVKDSHQPWTGLPLLTAAQQHQVSVEWGADFQSAFPRSPLSALFAEQVER
                     TPEALAVVFGDERLTYRELDRRAEALAERLREAGVGPETLVGLSTERSAALVVGMLGI
                     IKAGGAYVPLDPEYPRERIDFMVADAGVSLIVGEGLGIEAVQENPPPDLPRSLPPPSQ
                     GGRHPLPLGPDLAYVTYTSGSTGVPKGIAIPQTAVARLVLGTNYIDLGPGDRVAQVSN
                     ATFDAATFEVWGALLTGATLVGFPREVTLSPVRFAKALAETGVSVMFLTTALFHQVAR
                     EVPDAFASLRRLLVGGEAMDPRWGAEVLEKGAPGRLVNIYGPTEGTTFSTWHLIDTVP
                     PGRPVPIGRALANARTYVVDRELRPAPPGVAGELCLGGEGLARGYLRRPGLTAAAFVP
                     DPLSGLPGERLYRTGDLVRWAPEGTIEFLGRLDHQVKLRGFRIELGEIETALAACPEV
                     REAAVLMREVVPGDRRLVAYAVPRGTHDSAAVRSVLQSRLPDYMIPAALVWLDTLPLN
                     ANGKVDRRALLEISIGTEAAGEGRAAPRDLVEEQLAAIWEELLGVPRVGIHDDFFELG
                     GHSLLATRVVSRVREAFGVELPLRTLFAAPTVARLAGEVEEERRRGAGLASPPVIPVP
                     REGDLPLSFAQEWMWIADQLETEGGGYNAPMAVRLRGRLDVPALAAALDGVVRRHEAL
                     RTTFHSGEGGPVQRVQREMPVPLEEIDLGALPEMGEVERVAVAFSRWPFDLAIGPPLR
                     ACLVRLGGEDHVLLLTLHHLVTDGWSGGILLEDLAVLYRAASEGRPSPLPRLPVQYAD
                     FAVWQRRWLTGDVLERLVGYWRERLAGRPAALEIPTDRPRSAVRTQWGARLPVAFSGE
                     VSERFAALARQERVTPFTSLLALWKTLLARYSHQEDLVVGTPVANRARREVEGVVGYF
                     VNDLPLRTDLSGEPSFRDLLARVHETALGAHAHQDLPAVKLAQELQAGSGALFQAWFQ
                     LQNTPPLRIDLPGLAFAEHRAELPAASFELELALRETELGLEGYFSYNRDLFRRDTAE
                     AMLGHLLALAEGAAAEPDRPIWELSLLVDAEWRQVAAAGKVERYVAEVPLHRQFEARA
                     AKAPDRVAVTFEDGSLTYGELDRRAEALAGRLREMGIGPEARVGVSLPRSAELLVAIL
                     GVLKAGGAYVPLDPAYPRERLEMLVEESRVAVVVGASDIARSREGASPSPTGEREGTS
                     DPDHLAYVIYTSGSTGRPKGVMVTHRQVARLFEASGWFGFGAEDVWTLFHSYAFDFSV
                     WEIWGALLFGGRLVVVPYETSRSPEDFQELLVREGVTVLNQTPSAFQGLVRAEGPGHL
                     LPLRWVIFGGEALELRALVPWFRRHGEVRPRLVNMFGITETTVHVTLRPLTRADAEEG
                     AGSPIGEPLPDLRLYLLDRRLRPVPPGVVGEVYVGGGGVARGYLHRPDLTAERFVPDP
                     FTGIHGEAGARLYRSGDLARTRRDGELEYLGRADQQLKVRGFRIEPGEIEAALLGQPG
                     IREAVVLARDYSQGDRRLVAYLGTDDVPDLAALRASLLETLPEHMVPAAFVTLPALPL
                     TAHGKVDRRALLALEPEVRPVAETASEPPAGPLEEILTALWTDLLGAEAVGRDESFFA
                     LGGHSLLMTQVASRLKQLTGVTLPMRSFFEQPTVRGLAGSLLAEMVKATPVERLERLL
                     AEVEGVPEEAVGERIGEKRTSYARVLTPRPPLPSPTLPPPGEGETSGSEEKGFSPPLP
                     GSGRAGDGRGGRGVRTPGQDAAPHTALRVRDLLVLMPEGPAIPAPPPITAMGFPTANR
                     PEVLERGITSYAEATRDRGQPLRFVVTDDSRVPTIREE"
BASE COUNT          831 a         2317 c         2368 g         1017 t
ORIGIN      
        1 cccgtccttt cgatctcacc cgggggccgc tcctccgggg tctgctcctc cggaccacgg
       61 agcgaggggg tccggtccgc ccggcgcagg cgactggacg cgctggggga tgggctgggg
      121 gggcgcaccc gaaggagccg agcagggtcg gagcggaccc cctcgcggcc ggccaccacc
      181 accttctcgt cctgacgatg caccacatcg tctcggacgc ctggtccatg gacgtgctcg
      241 cccgcgagtt gggccttctc tacgcttccg agccgccgcc accgctgccc atccagtacg
      301 ccgacttcgc cctctggcag cgccgccacc tgtcctcgga gctactcgcc gaccatctca
      361 cttggtggcg ccaccgtctt gccggagcgc cgccgctcct cgacctgccg accgaccatc
      421 cccggccggc ggtgcgaacc cagcgtgggg acgagctctc cttccacctc ccggagcacc
      481 tctcccacac cctggagagc ctcagccacc aagagggcgc caccctcttc atgactctcc
      541 ttgccgcgtt ccaggtcctc ctggcccgtc tcgccaacca gcaggacgtg agcgtcggca
      601 ctcccatcgc cggccgccgc caggccgaga ccgagcccct gatcggcttc ttcgtcaata
      661 cccttgtgat gcggaccgat ctctccggcg agccggtctt ccggaccctg ctggggcggg
      721 tgcgggacat ggccctgggc acctacgccc accaggacct ccccttcgag cgtctggtgg
      781 aggagctggc ccccgagcgg aacctcgcgc acaccccgct gttccaggcc gtgttcaccc
      841 tccagcgccc accggccggc ctcggcaccc tgcccgggct ggaggtctcg ccggtacgtc
      901 tcggagcccg gacggccaag ttcgacctga ccctcacgct gatctacgag cccggcggcc
      961 tcgacggctg gatggaattc agcgccgatc tcttcgatcg cacgtccgtg cagcgctggg
     1021 ccggtcactt ccaggctctc ctcgagggag tggtgaagga cagccaccag ccctggaccg
     1081 gtctgcccct gttgaccgcc gcccaacagc accaggtcag cgtcgagtgg ggggccgact
     1141 tccagagcgc cttcccgcgt tccccccttt cagctctctt tgcagaacag gtggagagga
     1201 cgcccgaggc cctggccgtg gtcttcgggg acgagcggct gacctatcgc gagctggacc
     1261 gccgggccga ggcgctggcg gagcgtcttc gcgaagccgg agtcgggccg gagacgctgg
     1321 tggggctcag cacggagcgc tcggcggctc tggtcgtcgg catgctgggg atcatcaagg
     1381 ccggtggcgc gtatgtaccg ctcgacccgg agtacccgcg ggagcggatc gacttcatgg
     1441 tggcggatgc gggggtgtcg ctcatcgtcg gagaggggct tgggatcgag gcggtccaag
     1501 agaacccccc tcctgacctc ccccgctccc tcccaccccc ctcccagggg gggaggcacc
     1561 ccctccccct cgggcccgat ctggcttatg tgacgtatac gtcgggatcc acgggggtgc
     1621 ccaaggggat cgcgattcct caaaccgcgg tggcgcggct ggtactggga acgaactaca
     1681 tcgacctcgg gccgggtgac cgcgtggccc aggtctccaa cgccacgttc gacgccgcca
     1741 ccttcgaggt gtggggggcg ctcctcaccg gtgccacact ggtggggttc ccccgagagg
     1801 tgacgctgtc gccggtccgc ttcgccaagg cgctggccga gaccggcgtc agcgtcatgt
     1861 tcctcacgac ggccctgttc catcaggttg cccgggaggt gccggacgcc ttcgcctccc
     1921 tgcgccgcct gctcgtcggc ggtgaggcca tggacccgcg gtggggcgcc gaggtgctgg
     1981 agaagggggc gcccgggcgg ctggtcaaca tctacggccc taccgagggg acgacgttca
     2041 gcacctggca cctgatcgac accgtgccgc ccgggcgccc ggtgcccatc ggccgcgctc
     2101 tcgccaatgc acggacctat gtcgtggacc gcgagctgcg gccggccccg ccgggtgtgg
     2161 ccggtgagct gtgcctcggc ggcgaaggcc tcgcccgcgg ctatctccgg cggcccggcc
     2221 tgaccgcggc ggcgttcgtc cccgatcccc tctccgggct gccgggcgag cggctctacc
     2281 ggaccggcga cctggtgcgg tgggccccag aagggacgat cgagttcctg ggccgcctgg
     2341 atcaccaggt gaagctccgg ggcttccgca tcgagctcgg cgagatcgag accgccctgg
     2401 ccgcctgtcc ggaggtgcgg gaggccgccg tgctgatgcg ggaggtggtc ccgggagacc
     2461 gccggctggt ggcgtatgcg gtcccccggg gtacccatga ctcggccgcg gtacggtccg
     2521 tcctccagag tcgcctgccc gactacatga tccccgcggc gctggtctgg ctcgacaccc
     2581 tgccgctcaa cgccaacggc aaggtggacc ggcgggcgct gctggagatt tcgatcggca
     2641 ccgaggccgc gggagagggc cgagcggcgc cgcgcgacct ggtagaggag cagctcgccg
     2701 cgatttggga ggagctgctc ggggtcccgc gggtcggcat ccacgacgac ttcttcgagc
     2761 tgggcggcca ctcgctgctc gcgacgcggg tggtctcccg ggtgagggag gcattcggcg
     2821 tcgagctgcc gctacggacg ctgttcgctg caccgaccgt ggcgcggctc gccggcgagg
     2881 tagaggagga gcggcgccgg ggcgcgggcc tcgccagtcc gccggtgatc cccgtacccc
     2941 gtgagggcga cctccccctc tccttcgccc aggagtggat gtggatcgcc gaccagctcg
     3001 agaccgaggg gggcggctac aacgcaccga tggcggtgcg cctgcgcggc cgcctggacg
     3061 tccccgcgct ggccgcggcc ctcgacgggg tggtccgccg tcacgaggcc ctgcggacca
     3121 ccttccattc cggcgaggga ggccccgtcc agcgagtcca gcgggagatg ccggttcccc
     3181 tggaggagat cgacctcgga gccctgccgg aaatggggga ggtggagcgg gtggcggtgg
     3241 cgttctccag gtggccgttc gacctggcca tcgggcctcc cctgcgggcc tgcctcgtgc
     3301 ggctcggcgg cgaggaccac gtcctgctcc tcaccctcca ccacctggtc acggacggct
     3361 ggtcgggcgg catcctgctg gaggatctgg ctgtgctcta ccgggcggcg agcgagggcc
     3421 ggccttcgcc gctgccgcgc ctgcccgtgc aatacgcgga cttcgccgtc tggcagcggc
     3481 ggtggctgac gggcgacgtc ctggagcggc tggtcggtta ctggcgggag cggctcgccg
     3541 ggaggcctgc ggcgctggag atccctaccg accgcccccg ctcggctgtg cggacccagt
     3601 ggggtgcccg gctgccggtg gcgttctccg gggaggtctc ggagcgcttc gccgccctgg
     3661 cgcgccagga gcgggtgacg ccgttcacct cgctcctcgc cctctggaag accctcctcg
     3721 cgcgctacag ccatcaggag gatctggtcg tgggcacgcc ggtggcaaac cgcgcccggc
     3781 gggaggtgga gggggtggtc ggctacttcg tcaatgacct gccgctacgc accgacctct
     3841 ccggcgagcc gtcgttccgc gatctgctcg cccgggtcca tgagacggcg ctcggcgcgc
     3901 acgcccatca ggacctgccg gcggtgaagc tcgcccagga gctccaggcc gggagtgggg
     3961 ccctcttcca ggcctggttc cagctccaga acacgccgcc gctacgcatc gatctgccgg
     4021 ggctcgcctt cgccgagcac cgggcggagc tgccggctgc cagcttcgag ctggagctgg
     4081 ctctacggga gaccgagctc gggctggaag ggtatttctc ctataaccgg gacctgttcc
     4141 ggcgcgacac ggcggaggcc atgctcggcc acctgctcgc cttggctgag ggagccgcgg
     4201 cggagccgga ccgcccgatc tgggagctgt cgctgctcgt ggacgcggag tggcggcagg
     4261 tggcggcggc cggtaaggtc gagcgatatg tggcagaggt gccgctccat cggcagttcg
     4321 aggcgcgggc ggccaaggct ccggaccggg tggcggtgac gttcgaggac gggagcctga
     4381 cctatggcga gctggatcgc agggccgaag ctctggccgg gcggctccgg gagatgggga
     4441 tcgggccgga ggcgcgggtg ggagtctcgc tgccacgctc ggcggagctg ttggtggcga
     4501 tcctcggcgt gttgaaggcg ggaggcgcct atgtgcctct cgatccggcc tatccgcggg
     4561 agcggctgga gatgctggtc gaggagtcgc gggtggcggt ggtggtgggg gcctccgaca
     4621 ttgcccgctc ccgggagggg gcaagcccct cccctacagg agagcgggag gggaccagcg
     4681 atcccgatca tctggcctac gtcatctata catccggctc caccggaagg cccaaggggg
     4741 tcatggtgac ccaccgccag gtcgcgaggc tgttcgaggc gtccgggtgg ttcggcttcg
     4801 gggcggagga tgtctggacg ctcttccact cctatgcgtt cgacttctcg gtctgggaga
     4861 tctggggggc tctcctcttc ggcggccggc tggtggtggt gccttacgag acgagccgct
     4921 cgccggagga cttccaggag ctgctggtgc gtgagggggt gacagtcctc aaccagaccc
     4981 cctcggcgtt ccagggattg gtacgggcgg aggggcccgg acaccttctc cccctccgct
     5041 gggtgatctt cggtggcgag gcgctggagc tccgcgccct cgtcccctgg ttccggcgcc
     5101 acggggaggt ccggccccgg ctcgtcaaca tgttcggcat caccgagacg acggtgcacg
     5161 tcacccttcg cccgctgacc cgtgccgatg ccgaggaggg cgccggcagt ccgataggcg
     5221 agccgctgcc cgacctccgg ctctatctcc tggaccgccg cctccgcccg gtgccgccgg
     5281 gtgtggtggg ggaggtctac gtcggtggcg ggggtgtggc acgcggttat ctccaccgtc
     5341 cggacctgac ggccgagcgg ttcgtgcccg atccctttac cggcattcat ggcgaggccg
     5401 gagcgcgcct ttatcgctcc ggcgacctcg cccggacgcg gcgggacggt gagctcgagt
     5461 acctcggacg ggcggaccag cagctcaagg tccgcggttt ccgcatcgag ccgggcgaga
     5521 tcgaggccgc gctgctgggc cagccgggca tccgcgaagc cgtggtcctc gcccgcgact
     5581 actcccaggg ggaccgccgc ctcgtcgcct atctcggtac ggatgacgtc ccggacctcg
     5641 ccgcgctccg tgcgtcgctc ctggagaccc tccccgagca catggtcccc gcggccttcg
     5701 tgaccctgcc ggcgctgccg ctcacggcgc acggcaaggt ggaccgccgg gccctgctgg
     5761 ccctggagcc cgaggtccgg ccggtcgcgg agaccgcctc cgagcctccg gcgggccctc
     5821 tggaggagat cctcaccgcg ctctggaccg atctcctggg agcagaggcc gtcggccggg
     5881 acgagagctt cttcgccctc ggcgggcact cgctgctcat gacccaggtg gcctcgcggc
     5941 tcaagcagct tactggtgtg accctgccca tgcggagctt cttcgagcag ccgaccgtcc
     6001 gcgggttggc cgggtcgctc ctggcggaga tggtgaaggc gacgccggtg gagcggttgg
     6061 agcggttgct ggcggaggtg gagggggtgc cggaggaggc ggtgggggag aggattgggg
     6121 aaaagcggac gagctacgcg cgagtcctca ccccccggcc ccctctccca tctcccaccc
     6181 tccccccgcc aggagagggg gagaccagcg gcagcgaaga aaagggtttt tctccccctc
     6241 tcccggggtc cgggcgggcg ggagatggga gagggggccg gggggtgagg actccagggc
     6301 aggacgccgc gcctcacacc gcccttcgcg tccgcgacct cctcgtcctc atgccggagg
     6361 gaccggccat ccccgctccg ccgcccatca cggccatggg gttcccgacc gccaaccggc
     6421 cggaggtgct ggagcgcggg atcacgagct acgccgaggc gacacgggat cgcggccagc
     6481 ccctgcgctt cgtggtgacc gacgattcac gggtgcccac catccgcgag gag
//
LOCUS       DASPJI010000499         4464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_90549, whole genome shotgun sequence.
ACCESSION   DASPJI010000499 DASPJI010000000
VERSION     DASPJI010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90549"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VEL74_13545"
     CDS             complement(<1..1023)
                     /locus_tag="VEL74_13545"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HYG63600.1"
                     /translation="MSVRIACATLPLFPLAARLRSEPELTREAVAVVEGNGNAARVVA
                     ATRLARNAGVRPGLTLPQARALCPKLVARPRDEECEQAAQEALLEVAESYSPRVEDAG
                     DGVAFLDVEGIDRHFPGECPERELGRSLIATADGAGLAVRVGIASSKLAAQIAAGLPD
                     SPVIVPPGDEAGFLAPLPLTRLTPEVDLAETLERWGVRTVGDFARLPKSQVASRLGRT
                     GRDLHATARGMDARPLIPREPPPEFHEGMNLEWPLVSLEPFLFVGRAALERLCQRLES
                     RGLACTRLVLSLRLEPEGHQTRSIDLPAPTRDVKTLLTLIRLDLEANQPGAPVAGFTF
                     TAHPDRP"
     gene            complement(1046..1975)
                     /locus_tag="VEL74_13550"
     CDS             complement(1046..1975)
                     /locus_tag="VEL74_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63601.1"
                     /translation="MALPLIKETPSSPALTRLRGLLPQSLGSRLKSAGEMVRARREEE
                     KDTPFQTAVPAIDRLLEGGLPRGQLVELIGGRSSGRFSTELAVLAAATGTGETAALVD
                     LGDSLDPEAAIAAGVDLERLLWVRPQTMKQALAAAEMLLGSGFALVILDMGYPPVRGG
                     RGVEAAWLRLARAAESHTSALLVGSPYRVSGTAAAVVLKAGRSRVAWQGGGAAPWLLT
                     GLSAHVSLEKHRGRLTGQTEGLDLAVPESPLPSTPPQPGRGKGSANGAPAQATGREGG
                     ETGREHPKEPSTGPRSRSPWVEAERAPLRRAIA"
     gene            2271..2930
                     /gene="pdxH"
                     /locus_tag="VEL74_13555"
     CDS             2271..2930
                     /gene="pdxH"
                     /locus_tag="VEL74_13555"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HYG63602.1"
                     /translation="MSISGVRREYELGGLSEGEIAADPLAQFRVWLDQALEADPLDPT
                     AMTLATVDREGRPSARVVLLKGYDERGFVFFTNYESRKGRELDENPRAALVFFWPKFD
                     RQVRVEGAVERTSREESEEYFLSRPLGARLGAWASEQSRVIAGREELERRVREVTERF
                     GGGGAVPLPDFWGGYRVRPEVIEFWQGRPSRLHDRIVYIRLPGDSGEGGDGWRIERLS
                     P"
     gene            complement(2941..3522)
                     /locus_tag="VEL74_13560"
     CDS             complement(2941..3522)
                     /locus_tag="VEL74_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63603.1"
                     /translation="MNGKKILAAALVAGALAAVPAFAVKKTVEPEELWLHVKVHAAEG
                     DERTTINLPLSLVQGFIALVPDTARGSAKIRVNDREVSVPELRTMWRGLRHKADTTWM
                     TTDSRDGKMRIAKRGGYLYIQAEDRTGGNEQAEIRIPATVVDALLSGKDDELELGAAI
                     SALARHGEGELVAVNSENETVRIWVDGESVGRR"
     gene            3669..>4464
                     /locus_tag="VEL74_13565"
     CDS             3669..>4464
                     /locus_tag="VEL74_13565"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYG63604.1"
                     /translation="MSDALDRRRFLTLLSAAGAVPAVLAAQNQDNGEKKESVVTAGNL
                     AGQEKVIGLELSDTERDLMANGLAEQLEDYRRIRTVTIDNGVPPALEFDPLLPGARVE
                     VEKRPFRLSDEKAPAVSQNPEELAYLPVTQLSRLVRDRRVTSAALTRMYLARLKRLDP
                     VLHAVISFTEERALEQAEQADKEIAAGRYRGPLHGIPWGAKDLLAVKGYRTTWGSVPF
                     KEQMIDLDAAVVERLDKAGAVLIAKLTLGELAWGDVWFGGMTRNPWN"
BASE COUNT          636 a         1529 c         1673 g          626 t
ORIGIN      
        1 cggccggtcg gggtgggcgg tgaaggtgaa gcccgccacc ggcgctccgg gctggttcgc
       61 ttccaggtcc aggcggatca gggtgagcag cgtcttgacg tcgcgggtgg gcgccggcag
      121 gtcgatggag cgggtctggt gcccctcggg ctcgagccgc agggagagca ccagccgcgt
      181 gcaggcgagc ccgcgcgact ccagccgctg gcagagccgc tccagggcgg cgcggccgac
      241 gaacaggaag ggctcgagcg agaccagcgg ccactccagg ttcatcccct cgtggaactc
      301 gggcggcggc tcgcgcggaa tgagcggccg ggcatccatg ccgcgcgccg tggcgtggag
      361 gtcgcgcccg gtccgcccca accggctcgc cacctgggac ttgggcaggc gggcgaagtc
      421 gccgacggtg cgcacgcccc agcgctccag ggtctcggcc aggtccacct cgggggtgag
      481 gcgggtcagg ggtaggggag cgaggaagcc ggcctcgtcg ccggggggca cgatcaccgg
      541 cgaatccggc aagccggcgg cgatctgcgc cgccagcttg ctggaggcga tgcccacccg
      601 cacggccagt ccggctccgt cggcggtggc gatgagggag cgcccgagct cgcgctccgg
      661 gcactcaccg gggaagtggc ggtcgatccc ctcgacgtcc aggaaggcga cgccgtcgcc
      721 cgcgtcctcg acccggggcg agtagctctc ggcgacctcg agcagggcct cctgcgccgc
      781 ctgctcgcac tcctcgtcgc gcgggcgggc gaccagcttg gggcagagcg cccgggcctg
      841 ggggagggtg aggccgggcc gcacgccggc gttgcgggcg aggcgggtgg cggccaccac
      901 ccgggcggcg ttgccgttgc cctccaccac ggccacggcc tcgcgggtga gctccggctc
      961 gctccgcagc cgggcggcga gaggaaagag gggaagggtg gcgcaggcga tacggacgga
     1021 catgcggccg gacgatggtc aagggtcagg caatggctcg gcggagggga gcgcgctccg
     1081 cctccaccca cggggagcgc gaccggggac ccgtgctcgg ctccttcggg tgctcccgcc
     1141 cggtctcccc accctcgcgt ccagtcgcct gcgccggagc cccgttcgcg ctccccttcc
     1201 cccgcccggg ttggggagga gtcgaaggca gggggctctc ggggacggcg aggtcgagac
     1261 cctcggtctg gccggtgagg cggccgcggt gcttctcgag ggatacgtgg gcggagaggc
     1321 cggtcagcag ccagggggcg gcgcctcccc cctgccaagc gacgcgggag cgtccggcct
     1381 tgaggacgac ggcggccgcg gtgccgctca cccggtaagg gctgccgacg aggagcgccg
     1441 aggtgtgcga ctcggccgcc cgggccagcc gcagccaggc ggcctccacc ccccggccgc
     1501 cacgcaccgg cgggtagccc atatcgagga tgaccagggc gaagccgctg cccagcagca
     1561 tctccgccgc ggccagcgcc tgcttcatgg tctgcgggcg tacccagagc agccgctcca
     1621 ggtccacccc ggcggcgatg gccgcctcgg ggtcgaggct gtcccccagg tccaccaggg
     1681 ccgcggtctc gcccgtgccg gtggccgcgg cgaggaccgc cagctcggtg gagaaccggc
     1741 cgctcgaccg gccgccgatc agctccacaa gctggccgcg cggcagcccc ccttcgagga
     1801 ggcggtcgat cgccggcacc gccgtctgga agggggtatc cttctcctcc tcccgccgtg
     1861 cccgcaccat ctcgccggcg ctcttgaggc gcgaccccag ggactggggg agcagccctc
     1921 gcagccgggt cagcgccggg ctggaaggtg tctccttgat gagcggcagg gccatggcgt
     1981 atcctctcct cgtctcccta aatataaagc gtaatgatgg cgtaatcaat aggtgaggat
     2041 gttttaacat ccgctgtgga gccttgcgtc atggcggttt ccgaggcgat aaaaccgata
     2101 gataacaagg gtgggaagat ctttcttgtc cggaagtgaa ttgtggcggg aaggtccaac
     2161 ttcggcgggg cgggatttcc tccggctttc ctcaggacgg ggggcgtcca tagaatgggg
     2221 ccggccgtga gccgagagga gggatagcga tggcaggggg agaaggaccg atgagcatct
     2281 ccggcgtgcg ccgggagtac gagctgggcg ggctctccga gggggagatc gccgccgatc
     2341 ccctggcgca gttccgggtc tggctggacc aggccctgga ggcggacccg ctcgacccca
     2401 cggccatgac cctggccacg gtggaccggg aaggcaggcc gtccgcccgg gtggtgctgc
     2461 tcaaggggta cgacgagcgg ggtttcgtct tcttcaccaa ctacgagagc cgcaagggga
     2521 gggaactgga cgagaacccg cgggccgcgc tggtcttctt ctggcccaag ttcgaccgcc
     2581 aggtgcgggt cgagggggcg gtggagagga catcgcggga ggagtcggag gagtacttcc
     2641 tgtcccggcc gctgggggcg cggctcgggg cctgggcgtc ggaacagagc cgggtgatcg
     2701 ccggccgcga ggagctggag cggcgggtgc gcgaggtgac cgagcggttc ggcggcggcg
     2761 gcgcggtgcc cctgcccgac ttctggggcg gctaccgcgt ccgccccgag gtcatcgagt
     2821 tctggcaggg gcggccgagc cgcctccacg accgcatcgt ctacatccgc ttgccgggag
     2881 atagcggtga gggcggggac ggctggcgga tcgaacggct atcgccctga gccgtcccga
     2941 tcagcgacgg cccaccgact cgccgtccac ccagatgcgg acggtctcgt tctccgagtt
     3001 gaccgctacc agctcgccct cgccatgacg ggccagggcg gagatggcgg ccccgagctc
     3061 gagctcgtcg tccttgccgg agagcagggc gtcgaccacg gtcgccggga tgcggatctc
     3121 cgcctgttcg ttgccgccgg tccggtcctc ggcttgtata tagaggtagc cgccgcgctt
     3181 ggcgatccgc atcttgccgt cccgggagtc ggtggtcatc caggtggtgt cggccttgtg
     3241 gcgcaggccc cgccacatgg tgcgcagctc cgggacgctg acctcccggt cgttgacccg
     3301 gatcttggcc gagccgcggg ccgtatccgg caccagggcg atgaagccct ggaccagcga
     3361 caggggcagg ttgatggtgg tccgctcgtc cccttcggcc gcgtggacct tgacgtgcag
     3421 ccacagctcc tcgggctcca cggtcttctt caccgcaaag gcgggcaccg ccgccaacgc
     3481 accggccacc aacgccgccg ccaggatctt cttgccgttc atggtcgcca tcctttctcg
     3541 ggagctccat cgctcccacc tctacgacga gggaagcggc cgggaggttt caatccgcgg
     3601 cccggaccca tagaatcggg cggtccaccc gtcaccttgc gagtcatttt gcgaccacgg
     3661 aggccctgat gtccgacgcc ctcgaccgcc gccggttcct gaccctgctc tccgccgccg
     3721 gcgccgttcc cgccgtcctc gccgcccaaa accaggacaa cggcgagaag aaggagtccg
     3781 tggtgaccgc tggcaacctg gccggtcagg agaaggtgat cggcctggag ctcagcgaca
     3841 ccgagcggga tctcatggcg aacggcctcg ccgagcagct cgaggactac cggcggatcc
     3901 ggacggtgac gatcgacaac ggcgtccccc ccgccctgga gttcgatccg ctgctgcccg
     3961 gcgccagagt ggaggtggag aagcggccgt tccggctgtc cgacgagaag gccccggcgg
     4021 tgtcccagaa cccggaggag ctggcctacc tgccggtgac ccagctctcg cgcctggtgc
     4081 gcgaccgcag ggtgacctcg gcggcgctga cccggatgta cctcgcgcgc ttgaagcggc
     4141 tcgatcccgt gctgcacgcg gtgatcagct tcaccgagga gcgggcgctg gagcaggccg
     4201 agcaggcgga caaggagatc gcggccggcc gctaccgggg ccccctgcac ggcatcccct
     4261 ggggcgccaa ggacctgctg gcggtcaagg gctatcgcac cacctggggc tcggtgccgt
     4321 tcaaggagca gatgatcgac ctcgacgccg cggtggtgga gcggctggac aaggccggcg
     4381 cggtgctcat cgccaagctg accctagggg agctcgcctg gggggacgtc tggttcggcg
     4441 gcatgacgcg caacccgtgg aacc
//
LOCUS       DASPJI010000500         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_776888, whole genome shotgun sequence.
ACCESSION   DASPJI010000500 DASPJI010000000
VERSION     DASPJI010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776888"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VEL74_13570"
     CDS             complement(<1..823)
                     /locus_tag="VEL74_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63605.1"
                     /translation="MVLRASILLITFFLALAPGAPVGAQEKPRPGEITMPVAEYLALV
                     QRVEEVEKERAQRASRREPTVSEVVAQRTALTVGESEGQVTSDFEVVVQGYPDKPLLL
                     PVSGLAERVEVSPATASVTAARGGVTLVAPAPGRYAVKVQGRLPLDRSGGISRLTLAP
                     VVAPVAEVQAELPAELGWSSPGTVLVEDREEGARRTVRLAVSRGDARVLELRRRVDGG
                     EAERLLARSVVLTILQVRPEGTRRHDVVLYEVSRGGLPGFTVDLPAGLEVEQVGTD"
     gene            complement(814..2583)
                     /locus_tag="VEL74_13575"
     CDS             complement(814..2583)
                     /locus_tag="VEL74_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63606.1"
                     /translation="MRRLSGLAIVYLNLFLAAFGAAAQVTLPLEKFENLRARANPSPD
                     DAPPPPAPYALESADFDVRAGDTSARVIQRLTLTLYAPGWQKVPLGEAGSFIAADLGR
                     LEGRVAADGSGLSLEVRGSGRHEVRLESVMPLVRDETATRPTWRLRLRPPAAAVVRGR
                     LAAPTQVEEVLIEGTGLAHRLDGGGWSFVAAPSAPLGITLAGKRTLPEKARLPLRFEA
                     TSATGVTLSRTRLRARGWIEARVAQGRLPELRVPVPQGLEVVSVEGPVAGWDVADGVL
                     VITPLEPVESTLQVEVELAGEPRDAFATPLLVPQGSSRTLYFAKAALEGDGLLELADP
                     GAVRQPEEREVLAIPPGFREADGRAVALLDPARPPHWRAQWADRTEVLAAQVDRLLVD
                     VAVGESGRASYQLWAEIRNRGSQQLVVTLPGGFELAEGVRDGTPITAGATGDGLAVPL
                     RAAEEAQVVYIAGLLPLALPTGKGELMVPLPALSAPAARIEVRLVLPGGGRSYALADS
                     SRDGVIGAPPRVIVSSDRPPSSQIARQLFARRAVLADDDEPVLFYRPPGFFVMTAAWS
                     ALTAAPDPLMVRVEKEKERLEWF"
     gene            complement(2699..>3285)
                     /locus_tag="VEL74_13580"
     CDS             complement(2699..>3285)
                     /locus_tag="VEL74_13580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG63607.1"
                     /translation="GGLGLGLSISKTLVEMHGGRLTAASEGWGRGALFTVRLPLPEPG
                     ELEAEEAADPMAEAPVPRPAAAPARPLSILLVEDHPDTAAALADLLRAAGHQVTVRGT
                     VAEGKAAADEVYAAGGGFDLLLSDLGLPDGTGLELMRHLGGCYALPGIALSGYGMDED
                     LQASREAGFSRHLVKPVDLSTLMAAIGEVAAAKK"
BASE COUNT          479 a         1258 c         1151 g          397 t
ORIGIN      
        1 cgtccgtccc cacctgctcc acctccaggc cggccgggag gtccaccgtg aagccgggta
       61 gccctcctcg ggagacctcg tagagcacga cgtcatgccg ccgggtaccc tcgggccgca
      121 cctgaaggat ggtcaggacc acgctgcggg ccaggagccg ctcggcctcg ccgccgtcca
      181 cccgccgccg cagctccagc acccgggcgt cgccacgcga caccgcgagc cggaccgtgc
      241 ggcgcgcccc ctcctcccgg tcctcgacca gcaccgtgcc gggggagctc cagcccaact
      301 cggccggcaa ctcggcctgg acttcggcca ccggcgccac caccggcgcc aacgtcagcc
      361 ggctgatgcc gccgctgcgg tccagcggca gccgcccctg cacctttacc gcgtagcgtc
      421 cgggcgccgg cgccaccagc gtgacgccgc cgcgcgccgc ggtcaccgat gccgtggcgg
      481 gcgagacctc cacccgctcg gccaggccgg agaccggcag aagcagcggc ttgtccggat
      541 agccctggac gacgacttcg aaatccgagg tcacctgccc ctcgctctcc cccacggtga
      601 gagccgtgcg ctgggcgacg acctccgaga cggtgggctc gcgccgggag gcccgctgcg
      661 cccgctcctt ctccacctcc tcgacccgct gcaccagggc caggtactcg gcgacgggca
      721 tggtgatctc gccggggcgc ggcttctcct gggcgcctac cggtgcaccc ggcgccaggg
      781 cgagaaagaa ggtgatgagc aatatcgagg ctctcagaac cattccagtc tctccttttc
      841 cttctcgaca cggaccatga gggggtcggg cgcggcggtc agggcgctcc aggcggcggt
      901 catcacgaag aagccgggcg gacgatagaa gagcaccggc tcgtcgtcgt ccgccagcac
      961 ggcgcgccgg gcgaagagct gccgggcgat ctgcgaggag gggggacggt ccgacgacac
     1021 gatgacgcgc ggcggcgcac cgatgacgcc gtcgcgggag gaatcggcca gggcatagga
     1081 gcggccgccg ccggggagga ccagccggac ctctatacgg gcggcgggag ccgacagggc
     1141 cggcagcggc accatgagct cgcccttgcc cgttgggagc gccaaaggaa gcaggccggc
     1201 gatgtagacc acctgggcct cctcggccgc gcgcaacggc acggccaagc cgtcccccgt
     1261 agccccggcg gtgatcggcg tgccgtcccg caccccctcg gccagctcga acccgccggg
     1321 gagcgtcacc acgagctgct gcgagccgcg gttccggatc tccgcccaga gctgatagga
     1381 ggcgcgcccc gattccccca ccgccacgtc taccaacagg cggtccacct gcgccgccag
     1441 aacctcggtg cggtccgccc actgcgcccg ccagtgcggc ggccgggccg gatcgagcag
     1501 cgccacggcg cggccatccg cctcccggaa ccccggcggg atggcgagca cctcccgctc
     1561 ctcgggctgg cgcaccgctc cgggatcggc cagctccagc aggccgtccc cttcgagcgc
     1621 cgccttggcg aaatagaggg tgcgcgagct cccctgtggg acgaggagcg gagtcgcgaa
     1681 ggcgtcccgc ggctcgccgg cgagctctac ctccacctgg agcgtggact cgaccggctc
     1741 cagcggcgtg atcaccagga cgccgtcggc cacgtcccag ccggctacgg gtccctcgac
     1801 cgagaccacc tccagtccct gggggaccgg cacccgcagc tcggggaggc gcccctgggc
     1861 cacccgcgcc tcgatccagc cgcgcgcccg cagacgggtc cgtgacagcg tgacgccggt
     1921 ggccgaggtg gcctcgaagc gcagggggag gcgcgccttc tcgggcagtg tccgcttgcc
     1981 cgccagggtg atgccgagcg gcgccgacgg cgcggcgacg aagctccagc cgcccccgtc
     2041 caggcgatga gcgaggccgg tgccttcgat gagcacctcc tcgacctggg tgggggccgc
     2101 gagccggccg cgcaccaccg ccgctgcggg cggtcgcagg cggaggcgcc aggtgggccg
     2161 cgtcgcggtc tcgtcgcgga ccagcggcat caccgattcg agccgcacct cgtgccggcc
     2221 gctcccccgc acctccaggg agagcccgga gccgtcggcc gccacccggc cttcgaggcg
     2281 gcccaggtcg gcggcgatga acgaaccggc ctcgcccagc ggcaccttct gccagccggg
     2341 ggcatagagc gtcaaggtca gccgctggat cacccgggcg ctggtatcgc ccgcacgcac
     2401 atcgaagtcc gcgctctcca gcgcgtaggg cgcgggcggc ggcggtgcgt cgtctggcga
     2461 cgggttggcg cgggcgcgca gattctcgaa cttctccaaa ggcaaagtaa cctgagccgc
     2521 cgccccgaag gcagcgagga acaggttgag atagacgatg gccagacccg agagtctcct
     2581 catcggatga aacctcctgt tgacgagacc gctcccgcct cgtcgggatg acgttggagc
     2641 gaaccctgcc tagatagaca ccgcaggggc gcgagaagtt cctgagggga tggaccgcct
     2701 acttcttcgc cgccgccacc tcgccgatgg cggccatcag ggtggagagg tccacgggct
     2761 tgaccaggtg acgcgagaag ccggcttcac ggctggcctg gaggtcctca tccatgccgt
     2821 agccggacag ggcgatcccc ggcagggcgt agcaccctcc caggtggcgc atcagctcca
     2881 gccccgtgcc gtccgggagg ccgaggtcgc tcagcagcag gtcgaagccg ccgccggcgg
     2941 cgtagacctc gtcggcagcg gccttcccct ccgccaccgt gccccgcacc gtcacctgat
     3001 ggccggcggc gcgcagcagg tccgcgaggg cggcagcggt gtcggggtga tcctctacca
     3061 ggaggatgct caacggccga gccggggctg ccgcggggcg cggaaccggt gcctctgcca
     3121 tcgggtcggc ggcctcctcg gcctcgagct ctcccggttc cggcagcggc aaccggaccg
     3181 tgaagagggc gccgcgcccc cagccctcgc tggccgcggt gagccggccg ccgtgcatct
     3241 ccaccagcgt cttgctgatc gacagcccca gccccaggcc acccg
//
LOCUS       DASPJI010000501         2687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_776914, whole genome shotgun sequence.
ACCESSION   DASPJI010000501 DASPJI010000000
VERSION     DASPJI010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776914"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1703
                     /locus_tag="VEL74_13585"
     CDS             <1..1703
                     /locus_tag="VEL74_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HYG63608.1"
                     /translation="GGRSAMSLGLLALGFVAVLTVAYRLYGGWVARQFAIDDSRVTPA
                     HQVNDGVDFVPTRPFYLFGQHFSAIAAAGPIAGPIIAAQAYGWLPCLLWIGLGVVLIG
                     AVHDFSSLTASVRHGAHSIAEITGEHLGRKAGIAMMSFIWIALVYVIVAFVDITSRSF
                     VVGTEDLAAGTVAFHPGGAVAAASILYLLLAVVMGIVQRLWNPPLWVMTAIFVPAAFL
                     CPWVGTQMSTAILLDAKVWGVLILAYCFVASLVPVWSLLQPRGYLGGFILYAALALGV
                     IGIFFGGYEIQQEPFKTWDVGGMAGTLFPFLFVTIACGACSGFHGLVCSGTTSKQVDR
                     ESHMQPVGYGAMLAEGFVALIALVTVMIAAPAALKGLAPGTVYGNGIGEFLTLLIGDE
                     HRAFAVTFGAMAFSTFVFDTIDVSTRLGRYILQEVFGWSGKVGAIVATLLTVAAPALL
                     LLFAGEGMWAKFWTLFGASNQLLAALTLLSVTVWLYQARRRIAFTLLPMIFVLTITLW
                     ALTRLAWSNFQASQGLDVELLNAVASSLLILLALYLAIAALVKLRTERRTGQAVAAGV
                     "
     gene            2403..>2687
                     /locus_tag="VEL74_13590"
     CDS             2403..>2687
                     /locus_tag="VEL74_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63609.1"
                     /translation="MQSFDERSTSSIHPNIFTTQFLARLAERDEPPTAGDADVAGPWR
                     IEPVPGHGFGLFRIGESPARGFAPVVVFPSRWLALIAAAILPGTGRDPLLR"
BASE COUNT          369 a          903 c          913 g          502 t
ORIGIN      
        1 ccggaggccg gtccgcgatg agcctcggcc tgctcgccct cggcttcgtg gcggtcctga
       61 ccgtcgccta ccgtctctat ggcggctggg tggcgcgcca gttcgccatc gacgacagcc
      121 gggtgacgcc ggcccatcag gtgaacgacg gggtggactt cgtgcccacc cggccgttct
      181 acctgttcgg ccagcacttc tcggcgatcg cggcggcggg cccgatcgcc gggccgatca
      241 tcgccgccca ggcctacggc tggctcccct gcctgctctg gatcggcctc ggggtggtgc
      301 tgatcggcgc cgtccacgac ttttcgagcc tgaccgcctc ggtgcggcac ggcgcccact
      361 cgatcgccga gatcaccggc gagcacctgg gccgcaaggc cggcatcgcg atgatgtcct
      421 tcatctggat cgccctggtc tacgtgatcg tggcgttcgt ggacatcacc tcgcgcagct
      481 tcgtggtggg caccgaggat ctggcggcgg gcaccgtcgc cttccatccc ggcggcgcgg
      541 tggcggcggc gagcatcctt tatctattgc tggcggtggt gatggggatc gtccagcggc
      601 tgtggaaccc gccgctctgg gtgatgacgg cgatcttcgt cccggcggcc ttcctctgcc
      661 cctgggtggg gacgcagatg tcgacggcga tcctcctcga cgccaaggtc tggggggtgc
      721 tgatcctggc ctactgcttc gtggcgtcgc tggtgccggt ctggtcgctg ctccagccgc
      781 ggggctacct cggcggcttc atcctctacg ccgccctggc cctgggggtg atcggcatct
      841 tcttcggcgg ctacgagatc cagcaggagc cgttcaagac ctgggacgtg ggaggcatgg
      901 cgggcacgct cttccccttc ctcttcgtga ccatcgcctg cggcgcctgc tcgggcttcc
      961 acggcctggt ctgctcgggg accacctcga agcaggtgga ccgcgagtcg cacatgcagc
     1021 cggtgggcta tggagccatg ctcgccgagg ggttcgtcgc cctgatcgcc ctggtgacgg
     1081 tgatgatcgc ggcgccggcg gccttgaagg ggctggcgcc gggcacggtc tacggcaacg
     1141 gcatcggcga gttcctgacc ctcctgatcg gcgacgagca ccgcgccttc gcggtgacct
     1201 tcggcgccat ggcgttctcc accttcgtct tcgacaccat cgacgtctcg acccggctgg
     1261 gccgctacat cctgcaggag gtcttcggct ggtcgggcaa ggtgggagcg atcgtggcga
     1321 cgctgctgac cgtggccgcg ccggcgctgc tgctgctctt cgccggcgag gggatgtggg
     1381 cgaagttctg gaccctcttc ggagcctcga accagctcct ggcggcgctg acgctgctgt
     1441 cggtcaccgt ctggctctac caggcccggc ggcggatcgc cttcaccctg ctccccatga
     1501 tcttcgtcct gaccatcacc ctctgggccc tcacccggct cgcctggtcc aacttccagg
     1561 cctcccaggg gctggacgtc gagctgctga acgctgtcgc ctcgtcgctc ctcatcctgc
     1621 tcgctctgta cctggccatc gcggcgctgg tgaagctgcg gacggagagg cggacggggc
     1681 aggcggtggc ggcgggggtg tgagggggca ggggtatgct ctgggtgttg gaggagtgat
     1741 gatgtcgcga accctgccca ttccgccccc gaggaggcac ggcaagtcgc tgaggaatgg
     1801 caccaacgct ttgcaggacg gatcaccagt gacagcggcg agctgatccg cgaggaccgg
     1861 gacaggtgag atcgtaggtc agtgaacgcc ccgaaggggc acaggcataa agcctgccat
     1921 ttcaatggca ggtaggcgaa tccctaaata tttctagccc cgaaggggcg taggcaattc
     1981 ctcggcccac cgtgccgagt tgaagaatgc ctgcgcccct acggggcttt aaaatgtttt
     2041 tgctctttta cctgccattg aagtggcagg ctctatgcct gtgccccttc ggggcgttcg
     2101 gatggaccgg tttcgtcgta cgaggagacg gcgggccggc gtcgcacagg agcgggcaag
     2161 attctacgcg aaacctcttg actccccgcc cacctcctcc atagactctc ttcatcgaaa
     2221 acccggcgtg gtgtcttccc gggggaggag ggaacggagg gagagacgga agaaggatcg
     2281 gcaggccccg acccacatct ctggaaggcg ggacggtcgg catggtgccg gcttacggcc
     2341 cggaagcgag ctggtaactc gctcccagac ctaaccctac gaagtcccct gagggaggac
     2401 tcatgcagag cttcgatgag cgtagcacgt cgtctattca ccccaatatc ttcaccaccc
     2461 agttcctcgc ccgtctggcg gagcgggacg agccacccac ggcgggcgat gccgacgtcg
     2521 ccgggccgtg gcgcatcgag cccgttccgg gccacggttt cggcctgttc cggatcggcg
     2581 agagcccggc gcgcggcttc gcgccggtgg tggtgttccc ctcgcgctgg ctggccctca
     2641 tcgccgccgc catcctgccc ggtaccggcc gggacccgct gctccgc
//
LOCUS       DASPJI010000502         9999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_377554, whole genome shotgun sequence.
ACCESSION   DASPJI010000502 DASPJI010000000
VERSION     DASPJI010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9999)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9999
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377554"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VEL74_13595"
     CDS             complement(<1..268)
                     /locus_tag="VEL74_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63610.1"
                     /translation="MQTEVEPQVHKSLALAALFEEIRKGNKPRILDLGSAVGSNVEFL
                     SQFGCKLHIEDLYAALTSRNQVEIGELAGPDFFAGFLAPPEDVRF"
     gene            complement(316..846)
                     /locus_tag="VEL74_13600"
     CDS             complement(316..846)
                     /locus_tag="VEL74_13600"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HYG63611.1"
                     /translation="MAWKRSDDYPSQPEPAPAPARPVTPAPAPAATAYDAPRRTDRAT
                     IGPSIFIIGDLTGDEDLVIEGRVEGKVDLKQNNVTVGKNGRVRADVFGRVVTVEGEVD
                     GNVFAQEQAFLRQSGAIRGNITAPRVVLEDGSRFKGSIDMEPKDTGSAGNRQQSAKEN
                     QAKPAANAEAGKPVAV"
     gene            complement(1020..1448)
                     /locus_tag="VEL74_13605"
     CDS             complement(1020..1448)
                     /locus_tag="VEL74_13605"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HYG63612.1"
                     /translation="MPVRKANAVWEGDLKGGQGRVALGSGAYEGRYSFGSRFEEAGGT
                     NPEELIGAAHAGCFSMALSAGLGRAGFTPTRVATEAKVHLEKVGEGFKITRIELDNQS
                     EVPGIDEAAFQEQARKAKEGCPVSQALGGVEITLNARLSS"
     gene            complement(1479..1892)
                     /locus_tag="VEL74_13610"
     CDS             complement(1479..1892)
                     /locus_tag="VEL74_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HYG63613.1"
                     /translation="MRRTAATFLLAVLALVTQLAACSPAAERGHRFTVRGQVTQLPSP
                     QDPGALYLQHEAIDGWVDRNGQVVGMDPMNMPFPLGDGVPLEGIAAGDVVEATVRVDW
                     EAEARQVEIVELRELPAGTKLELRAADPGRAGTSH"
     gene            2011..3681
                     /locus_tag="VEL74_13615"
     CDS             2011..3681
                     /locus_tag="VEL74_13615"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63614.1"
                     /translation="MSERSDGLRLGGYAGAVEERLRRWQDERFGERLWARDHTLWSRE
                     PVPELTDRLGWLELPAAMAGEVETLERFAREAAEEGIRQAVVLGMGGSSLAPEVYART
                     FGAASGYPELEVLDSTHPGAVQDLAARLDATSDVERSLFLVSSKSGTTTETLSFFRFF
                     WERAAALGDPGRHFAAITDPGTPLEKMARERGFRAVFNAQPDVGGRYSALTPFGLVPA
                     ALLGADVRALLGSGWAMAEACAPNASAAANPGLLLGAALGELALAGRDKVTFVTSPSL
                     ASFPAWLEQLIAESTGKTGLGIVPVAGEPLGDPGVYGADRFFVAILLEEDDAGDIEER
                     LAALERDGHPVAVFRLGRKADLGAEMFRWEVAVAAAGAVLGLNPFDQPDVQLAKELAT
                     RAMKERGQGNAAVSSGGEVKAEALDGAMEDWLAGVGPGSYLGLHAYLPPRPATDEALS
                     RLQALLRDRTRCATTLGHGPRFLHSTGQLHKGGPDTGRFLQIVDEPADDLPVPEAGYT
                     FATLIRAQADGDRAALLQRGRRVLRVQLGQDAPAGLAALIGAVERLPS"
     gene            3678..4670
                     /gene="glk"
                     /locus_tag="VEL74_13620"
     CDS             3678..4670
                     /gene="glk"
                     /locus_tag="VEL74_13620"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HYG63615.1"
                     /translation="MILGGDVGGTKSNLGLFEVVEGGRLHLIRTATFANAEYPGLGVL
                     LGAFLGGDVPRVRAAAFGIAGPVLDNRAETTNLTWVIDGPGLAKETGIPRVLLINDLV
                     ATAEGIPLLGEDEVVTLQPGAAGAHGNRVLIAAGTGLGMSLLPWVDGRWVPVASEGGH
                     ADFAPRDADEEGLLRHLQARLGGRVSVERVVSGPGLFNIYEYLRDDAGMPELPEVRAA
                     LAAGDDPVPVISESGLTQRCGLCSRALDLFMAAYGAAAGNLALVGTATGGVYVGGGIA
                     PKVLPRIQEGGFLQAFVDKGRFEGYVRAIPVRVILNQKTAMYGAARVASLLRVG"
     gene            complement(4707..5588)
                     /locus_tag="VEL74_13625"
     CDS             complement(4707..5588)
                     /locus_tag="VEL74_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015180328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HYG63616.1"
                     /translation="MAQTTTRVGGIEQAGRQAGQVGRQAGQAVKSAVRSPWVEYLARF
                     GYAARGVVYAFVGFLAFQAAFGMGGQTTGTRGALSEIAEKSRILLGFVAVGLIGYALW
                     RFVQAVLDPEGKGDEAKGLAKRTMMLASGIVYSSLAVAAVRLLTGNGGGGTGDGAQQA
                     TAGILGKPFGVALVAIAGLAIIAAGLNELKNGWTQKFRNKLKLNEMSPAEQTWMTRTG
                     IAGLIARGVVFLLTGTFLVQAAMSSDPSRARGLQGALETVASQPYGTWLLALMGIGLL
                     AFGAYSLFLARYRRIYF"
     gene            complement(5669..5866)
                     /locus_tag="VEL74_13630"
     CDS             complement(5669..5866)
                     /locus_tag="VEL74_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63617.1"
                     /translation="MLKSFRKLLLVGIVFAASLTPATGQKAFHCPGVNFCALGCPQGW
                     TCDYLSCWCVECPAGYCGGDS"
     gene            complement(5950..6168)
                     /locus_tag="VEL74_13635"
     CDS             complement(5950..6168)
                     /locus_tag="VEL74_13635"
                     /inference="COORDINATES: protein motif:HMM:NF020908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HYG63618.1"
                     /translation="MSQERFSRRAVQRIAENRINQAIEEGKFDDLPGLGQPIPDIDEP
                     YDPMWWAKQWIRREKMGKLLAGNLKDWR"
     gene            6181..6327
                     /locus_tag="VEL74_13640"
                     /pseudo
     CDS             6181..6327
                     /locus_tag="VEL74_13640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6334..6459
                     /locus_tag="VEL74_13645"
     CDS             6334..6459
                     /locus_tag="VEL74_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63619.1"
                     /translation="MSCPHCGESYLTAETLHEIERIKLHRLDFAERRPVAVAAFA"
     gene            6740..7291
                     /locus_tag="VEL74_13650"
     CDS             6740..7291
                     /locus_tag="VEL74_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63620.1"
                     /translation="MRGYGQGNTPSTQTPNAFTASFLDRLAERDEPPIAGDADVAGPW
                     RIDALPSGGFGLFRMGESLARGFAPVTVFPSRWLALLHAAVLPGTGRDPLLLLSKEPD
                     AGGAYPLTLAGGGDDGGDGPHDGPVGLFQLFDEKSVDAMNTLIHLLRSPESLAYVLEA
                     AGSVTLERCGVILDERTAAEPSR"
     gene            7405..8445
                     /locus_tag="VEL74_13655"
     CDS             7405..8445
                     /locus_tag="VEL74_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63621.1"
                     /translation="MDAIQTLRNLPGRAGSHPLQVAGVQKTREASERLGWRPIQSDGV
                     RGTREAPGRSGRRTLQSDGSGAKLKFMNARKYLLGPLLLAGLPFAAGAAPERVPAWLV
                     SKSGGEAPPVWISAAAAVDDETVLKWDLLVDDTLKSTVQSQRQSLQKRGIPVEGVDGF
                     QIRSIAAEDCRSGILFSHMFENQPSGSLDDLLRFSRAIVRGTVRAVEPGIFYGKLVGS
                     LLQVQVEEVLRDAHEYGRSPSLYVYYPSGYFAVGPYRFCGHSTGHEAKIGDRVLVFDF
                     YGPKDRNGSLLIPRDSQVFFETPDGLLFLPREMSQDPALKSVNSLQGLAETVRRAPYS
                     GEPEKLDPNLIF"
     gene            complement(9050..>9999)
                     /locus_tag="VEL74_13660"
     CDS             complement(9050..>9999)
                     /locus_tag="VEL74_13660"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HYG63622.1"
                     /translation="AANAEGEVEIRDGAVCAQASKAALPRLLPLLLDIGQRLTQNLDV
                     ARRLADNARRDPLPGVRLRNLLVLARERPGEPVTRDALRAACSDPDPQIRLRAAAELG
                     AEGHETLIALAASTEDDDTWSAQAVSALAQHLPLERMKAILGQALRSRRLETARACLT
                     SLGRWRAAAIGELVKVLESEQDDLAAAAALALGGTGEASAEPPLLRALEHFSPEIQIA
                     AATTLGRAGSVETVLPLQEAAERGGRDVRRAAHQAIAEIQARLQDAAPGQLSLAANEA
                     GQLSLPEAEAGQLSLAPEAPGQLSSRPERPVAVPIQPPR"
BASE COUNT         1451 a         3408 c         3568 g         1572 t
ORIGIN      
        1 cgaagcggac gtcttcgggg ggcgccagga agccggcgaa gaagtcgggc cccgccagct
       61 ccccgatctc cacctggttg cgcgaggtca gcgccgcgta gaggtcctcg atgtgcagct
      121 tgcagccgaa ctgggagagg aactcgacgt tcgagcccac cgccgagccc aggtccagga
      181 tacgcggctt gttgcccttg cggatctcct cgaagagggc cgccagtgcc agcgacttgt
      241 ggacctgggg ctcgacctcg gtctgcaccg ggccctgctc cttgcggggg cccgaaccgc
      301 gagaaaacca gcccatcaca ccgcgaccgg cttacccgcc tcggcgttgg ccgctggctt
      361 ggcctggttc tccttggcgg attgctggcg gttgcccgcg ctgccggtat ccttgggctc
      421 catgtcgatg ctccccttga agcgggagcc gtcctcgagg accacacggg gcgccgtgat
      481 gttgccccgg atcgctccgg actgccgcag gaacgcctgc tcctgggcga agacgttgcc
      541 gtccacctcg ccctccacgg tcacgacgcg gccgaagacg tcggcgcgca cgcggccgtt
      601 cttgcccacc gtcacgttgt tctgcttgag atccacctta ccctcgaccc gtccctcaat
      661 caccagatcc tcgtcgccgg tcaggtcgcc gatgatgaag atggaggggc cgatggtcgc
      721 gcggtcggtc cgccgcggcg cgtcgtaggc tgtcgcggcc ggggccggag ccggggtcac
      781 ggggcgcgcc ggagcggggg ccggctcggg ctggctgggg taatcgtcac ttcttttcca
      841 cgccatgggt gaatctcctt ctgacaacaa gtcgtgatgt accagggagg gtaggggccg
      901 gaaggatgga agtcaaggat caaatagaaa tttcactatg ggaagggaga cgagggcggc
      961 ggggcctcta gggccccgcc ggattgatca cggagtgatc accgctgcgc agcggctgat
     1021 caactggaaa ggcgcgcgtt cagcgtgatc tcgacgccgc ccagcgcctg ggagacgggg
     1081 cacccctcct tggctttgcg ggcctgctcc tggaaggcgg cctcgtcgat gcccggcacc
     1141 tcggactggt tgtcgagctc gatgcgggtg atcttgaacc cctcgcccac cttctccagg
     1201 tgcaccttgg cctcggtggc cacccgcgtc ggcgtgaagc cggcgcggcc cagcccggcg
     1261 gagagggcca tggagaagca gccggcgtgc gcggcgccga tcagctcctc ggggttggtg
     1321 ccccccgcct cctcgaaacg ggagccgaac gagtagcggc cctcgtaggc gccgctcccc
     1381 agtgcgaccc tcccctgtcc ccccttgaga tctccctccc agacggcgtt cgctttacgt
     1441 acaggcatgt ggctctcctt tcgacgttga tgcacgattc agtggctggt gccggccctg
     1501 cccggatcgg cggcgcggag ctcgagcttg gtcccggcgg gcagctcccg gagctccacg
     1561 atctccacct ggcgcgcctc cgcctcccag tcgacccgga ccgtggcctc gaccacgtcc
     1621 ccggccgcga taccctccag cggcacgcca tcccccaagg ggaagggcat gttcatcggg
     1681 tccatgccca ccacctggcc gttgcggtcg acccagccgt cgatggcctc gtgctggaga
     1741 tacagggcgc cggggtcctg agggctgggg agctgggtga cctggccgcg gacggtgaac
     1801 cggtggcccc gttcggcggc cggcgagcag gcggcgagct gggtcaccag ggcgaggacg
     1861 gcgagcagga aggtcgcggc ggtccttctc atcggggccg gatcgtacca tgaggcccgg
     1921 ccccgccaga gaatggaagg gaagttgcat ttccgccgga gacggactcg aatatcggac
     1981 tcgaacggtg ggccgagtga aggaggagcg atgagcgaga ggagcgacgg gttgcggctg
     2041 ggagggtacg cgggagcggt ggaggagagg ctccggcgct ggcaggacga gaggttcggg
     2101 gagcggctct gggccaggga ccacacgctc tggtcgcgcg agcccgtgcc ggagctgacc
     2161 gaccggctgg gctggctgga gctccccgcg gccatggccg gagaggtgga gaccctcgag
     2221 cgcttcgccc gcgaggctgc ggaggagggc atccggcaag cggtcgtcct gggtatgggg
     2281 ggatcgagcc tggcgcccga ggtctatgcc cgcacgttcg gggcggcctc cggctatccg
     2341 gagctggaag tgctcgacag cacccacccc ggcgccgtcc aggacctggc ggcgcggctc
     2401 gacgccactt cagacgtaga gcgcagcctc ttcctggtct ccagcaagtc gggcaccacg
     2461 acggagacgc tctcgttctt ccggttcttt tgggagcggg cggccgccct cggagatccc
     2521 ggccggcatt tcgcggccat caccgacccc gggacgccgc tcgagaagat ggcccgcgag
     2581 cgaggcttcc gggccgtgtt caacgcccag ccggacgtcg gggggcgcta cagcgccctg
     2641 acgccgttcg ggctggtgcc ggcggccctg ctgggcgccg acgtccgggc cctccttggc
     2701 agcggctggg cgatggcgga ggcgtgcgcg cccaacgcgt cggcggcggc gaaccccggg
     2761 ctgctcctcg gcgccgcgct cggcgagctg gccctcgccg gccgcgacaa ggtcaccttc
     2821 gtcacctcgc cctccctcgc ctccttcccc gcctggctgg agcaactcat cgccgagagc
     2881 accggcaaga ccggcctggg catcgtcccg gtcgccggcg agccgctggg ggaccccggc
     2941 gtctatggcg cggaccgctt cttcgtcgcg atcctcctgg aagaggacga cgccggggat
     3001 atcgaggagc ggctcgcggc gctggagcgg gacgggcatc cggtcgcggt gttcaggctc
     3061 ggccgcaagg cggacctcgg cgccgagatg ttccgttggg aggtggcggt cgccgccgcc
     3121 ggcgcggtcc tcgggctcaa ccccttcgac cagccggacg tgcagctcgc caaggagctg
     3181 gcgacgcggg cgatgaagga gcgggggcag gggaacgcgg cggtctcgtc cggcggcgag
     3241 gtgaaggccg aggccctgga cggcgctatg gaggactggc tcgccggcgt gggtccgggg
     3301 agctacctgg gcctccatgc ctacctgccg ccgcggcccg cgaccgacga ggcgctgagc
     3361 cgcctccagg ccctgctgcg cgaccggacc cgctgcgcca ccacgctcgg ccacggcccg
     3421 cgcttcctcc actccacggg ccagctccac aagggcggcc cggacaccgg ccgcttcctc
     3481 cagatcgtcg acgagccggc ggacgacctg ccggtaccgg aggccggcta caccttcgcc
     3541 accctgatcc gcgcccaggc ggacggcgac cgcgccgccc tcctccagcg cggccggcgg
     3601 gtgctgcgcg tccaactcgg ccaggacgcc ccggccgggc tcgccgcgct gatcggcgcg
     3661 gtggagcggc tgccgtcgtg atcctcgggg gagatgtcgg cgggaccaag agcaatctcg
     3721 gccttttcga ggtggtggag ggggggcggc tccatctgat ccggaccgca accttcgcca
     3781 acgcggagta ccccggcctc ggcgtcctgc tcggggcgtt cctgggcggc gatgtcccgc
     3841 gggtccgcgc cgccgccttc ggcatcgccg ggccggtgct ggacaaccgc gccgagacga
     3901 ccaacctgac ctgggtcatc gacggccccg ggctcgccaa ggagacgggc atcccccggg
     3961 tcctgctgat caacgacctg gtggcgacgg cggaggggat cccgctcctc ggcgaggacg
     4021 aggtggtcac cctgcagccg ggggcggccg gagcgcacgg caaccgggtg ctgatcgcgg
     4081 ccggcaccgg cctggggatg tcgctgctgc cgtgggtcga cggccgctgg gtgccggtcg
     4141 cctccgaggg ggggcacgcc gacttcgcgc cgcgcgacgc ggacgaagaa ggcctgctgc
     4201 gccacctcca ggcgcgcctc ggcggccggg tcagcgtcga gcgggtggtc tcgggtcccg
     4261 gcctcttcaa tatctatgag tatctgcggg acgacgccgg gatgccggag ctccccgagg
     4321 tgcgggcggc gctcgccgcg ggcgacgacc cggtgccggt gatcagcgag tcgggcctca
     4381 cccagcgttg cgggctgtgc agccgggcgc tggacctctt catggcggcc tacggcgcgg
     4441 ccgccggcaa cctcgccctg gtgggcaccg ccaccggtgg cgtgtacgtc ggcggcggca
     4501 tcgcgcccaa ggtgctgccg cgcatccagg aggggggctt cctccaggcc ttcgtcgaca
     4561 aggggcgctt cgagggctac gtgcgggcga tcccggtgcg ggtgatcctc aaccagaaga
     4621 cggccatgta cggcgccgcc cgcgtggcct ctctcctccg cgtggggtag agaggctggc
     4681 gggcggcagg ggacaggttc cgcgctctag aaatagatgc ggcgatagcg ggcgaggaac
     4741 agcgagtagg cgccgaaggc gaggaggccg atgcccatga gggccagcag ccaggtgccg
     4801 tagggctggc tcgccacggt ctccagggct ccctggaggc cacgggcgcg gctcggatcc
     4861 gagctcatcg ccgcctgcac caggaaggtc ccggtgagga ggaacactac gccgcgggcg
     4921 atgaggccgg cgatgccggt gcgggtcatc caggtctgtt cggccgggct catttcgttg
     4981 agcttgagtt tgttccggaa cttctgggtc cagccgttct tgagctcgtt gaggccggcg
     5041 gcgatgatcg ccaggccggc gatggcgacc agggcgacgc cgaacggctt gccgaggatg
     5101 cccgcggtcg cctgctgggc gccgtccccg gtgccgccgc cgccgttgcc ggtgagcagc
     5161 cgcaccgcgg cgacggccag cgagctgtag acgatgccgc tcgccagcat catcgtccgc
     5221 ttggcgagcc ccttggcttc atcccccttg ccttcggggt cgaggaccgc ctggacgaag
     5281 cgccacagcg cgtagccgat cagtccgacg gcgacgaagc cgaggaggat gcggctcttc
     5341 tcggcgatct cggagagggc gccgcgggtg ccggtggtct gcccgcccat gccgaaggcg
     5401 gcctggaagg cgaggaatcc gacgaatgcg tacactacgc cgcgcgccgc gtatccgaag
     5461 cgggccagat actccaccca cgggctgcgg acggcgctct tcaccgcctg tcccgcttgc
     5521 cgccccacct ggcccgcctg cctcccggcc tgctcgatcc cacccacccg cgtagtcgtc
     5581 tgagccatgg tattgctcct ttccggcggg cggagcccgc acggaaaggg ggtagggcaa
     5641 cttctatgcc agggactcag ggggattgct aggagtcgcc gccgcagtac ccggccgggc
     5701 actcgacgca ccagcaactg aggtagtcgc aggtccagcc ctgcgggcag cctagtgcac
     5761 agaaattgac accggggcag tggaacgcct tctgtcccgt ggccggggtc agactggcgg
     5821 caaagacgat tccgaccagc agcagcttcc gaaagctctt gagcatctga tacctcctcc
     5881 tccatgcatg gcagcgggag cgctgcgcac gcggccctct ctgaagaaga ggtgcccggg
     5941 aggctcggct cagcgccagt ctttgaggtt gccggcgagc agcttgccca tcttctcgcg
     6001 gcggatccac tgcttggccc accacatggg gtcgtagggc tcgtcgatat cggggatcgg
     6061 ctggccgagg cccgggaggt cgtcgaactt cccctcctcg atcgcctggt tgatgcggtt
     6121 ctctgcgatg cgctgaactg ctctgcgaga aaaacgttcc tgactcatag ccgagtcagt
     6181 atgccagggt gggcgggaag gtcaagcttg gatcgggtag catgtcttca tgctggtctg
     6241 cgaaatctgt ggcaaggaag gggccgaatc cgccacgtct ccaggagcta tggcaagggg
     6301 gcggatctgc tggtcatcga gaacgtgccg gtggtgagtt gccctcattg cggtgagagc
     6361 tatctgaccg cggagacgct ccacgagatc gagcgcatca agctgcatcg gctggacttt
     6421 gccgagcggc ggccggtggc ggtggcggcg ttcgcctgag atctagcggg gggcggtggt
     6481 gcctcccccc ctgggagggt cgggagggag ggagcggggg aggacaggag gggggctttt
     6541 ttctttttcc gcccccttga caccccacaa aacttcacac tacgatcccg acatcaggac
     6601 gtctctgatc gcgatccgcg gtctccgcat cgggcgggat cgcacgctcc aggatgtctt
     6661 gaccaccgtg gtgaacggcc cggagtagag cggcaactct ctccgagcct gaccacacaa
     6721 cttccttgca ggaggaaata tgcgcggcta cgggcagggt aacactccgt ccacccaaac
     6781 ccccaacgcc ttcaccgcct cgttccttga ccgtctggcc gagcgggacg agccgcccat
     6841 agccggcgac gccgacgtcg ccggcccgtg gaggatcgac gcccttccaa gcggaggctt
     6901 cggcctcttc cggatggggg agagcctcgc ccgcggcttc gccccggtga cggtcttccc
     6961 ctcccgctgg ctggcgctgc tccacgccgc ggtcctcccc ggcaccggcc gcgacccgct
     7021 cctcctcctc tccaaggagc ccgacgccgg tggcgcctat cccctcaccc tcgcgggtgg
     7081 aggagacgac ggcggcgacg gccctcacga tggcccggtg ggtctcttcc agctcttcga
     7141 cgagaaatcg gtggatgcca tgaacacgct gatccacctc ttgcggtcgc cggagtcgct
     7201 ggcgtacgtg ctggaggcgg ccggctcggt caccctggag cgctgcgggg tgatcctcga
     7261 cgagcggacg gccgccgagc cgtcccggta gtacagcgac cgatcccgtg gcctgctccg
     7321 ccgcccggga gcaggccacg cgcttcggat ctcgtccgga cgctccggac gccgtccgtg
     7381 cgccgtggat ctcatccgaa agccgtggac gcgatccaga cgctccggaa tcttcccgga
     7441 cgcgccggat cgcatccgct tcaagtggct ggagtccaga agactcggga agcatccgag
     7501 cggctcggat ggcgtccaat tcaatcggat ggagtccgag gaactcggga agctcccgga
     7561 cgctccggac ggcgtacgct tcagtcggat ggctcaggtg ctaaactgaa attcatgaat
     7621 gcacgcaagt acctcttggg ccctctgctc ctcgccggac ttcccttcgc ggcaggcgcc
     7681 gctccggagc gcgtgccggc atggctggtc tcgaaaagcg gaggcgaggc gccgcctgtg
     7741 tggatctcgg ccgcggcggc ggtggatgac gagacggttc tgaaatggga cctgctggta
     7801 gacgacaccc tcaagagcac cgttcagtcg cagcggcaga gtcttcagaa acgcgggata
     7861 cctgtagaag gggttgatgg ttttcagatc cgctccatcg ccgccgagga ttgcagatct
     7921 ggaatcctct tctcccatat gttcgagaat caacccagcg gcagcctgga cgatctcctc
     7981 cgcttctccc gtgccatcgt ccgcggaacg gtgcgcgccg tcgagcccgg gatcttctat
     8041 ggcaagctcg tgggatcgct gctccaggtc caggtcgaag aggttcttcg tgacgctcac
     8101 gagtacgggc ggtcgccctc tctgtacgtc tactacccgt caggctactt cgcggtgggg
     8161 ccctatcgat tctgtggaca ctccaccggg cacgaggcca agatcggaga ccgcgtcctg
     8221 gtcttcgatt tctacggccc caaggaccgg aacgggtcgc tcctgatccc gagagactcc
     8281 caggtcttct tcgagacacc ggacggcctt ctcttcctcc cgagggagat gagtcaggat
     8341 cctgctctca agtccgtgaa cagccttcaa ggattggctg agaccgtgcg ccgtgccccc
     8401 tactccggcg agccggagaa gctcgatccg aatctgatct tctaggggcc cggatgggga
     8461 tgctgaggcg cggtgctcgg actggcggga gtgaagcccc gccggcagat cccgtgacga
     8521 tcccgttgct tgcttcgcca ttcccgctca tccctgagcc ctccccgtag atctcggagc
     8581 cgttcccacc tgagttggag cccttccacc ctagagtgaa gaggtcccac cctggggtgg
     8641 aggtgctcca tcctgagtgg gagcggtcca accctgaatg ggaggctctc cacgctgaag
     8701 cgacggtgtc caaccctgga caggaggggg ccaacctcga agcggaggct tcccaccctg
     8761 gagcggagag gccccaccct gagcaggcgc ctctccaccc tgagtcggga aggctccgga
     8821 atctgtggcg acggctccgg tctgaaggcg aaccgctcgt gctcagggtg gagaggctcc
     8881 cggctgaagg cgagcggctc ctgaccgagt gggaacagct acggcctgaa ggcgggcggc
     8941 cgcgggccgg ggctggacga ttccagtctg aaggcggagg gctctgtaac aggcggcaga
     9001 gggcgcggcc tgaaggggga cggctccgcc tcaggggcag gtcactcctc tacctggggg
     9061 gctggatcgg gacggccacc ggtctttccg ggcgggaaga gagctgcccg ggagcctcag
     9121 gcgccagcga cagttggccc gcctcggcct ccggcagcga gagctggccg gcttcgttcg
     9181 cggcgagcga caactgaccg ggcgcggcgt cctgaaggcg ggcctggatc tcggcgatcg
     9241 cctggtgcgc ggcccggcgg acgtcccggc ctccgcgctc ggcggcctct tgcagcggca
     9301 gcacggtttc caccgagccg gcacgcccca gcgtggtggc cgcggcgatc tggatctccg
     9361 ggctgaagtg ctccagagcc cggaggagcg gcggctcggc ggacgcctct ccagttcctc
     9421 ccagggcgag agccgcggcg gcggccagat cgtcctgctc gctctccagc accttcacca
     9481 gctcgccgat ggccgcggcc cgccatcgtc cgaggctcgt caggcaggcg cgcgccgttt
     9541 cgaggcggcg gctccgcaag gcctggccga ggatggcctt catacgctcg agcggcaggt
     9601 gctgggccag agccgagacc gcctgggcgc tccaggtgtc gtcgtcctcc gtgctcgccg
     9661 cgagcgcgat cagggtctcg tgcccctcgg ctcccagctc ggcggcggcg cgcaggcgga
     9721 tctgcgggtc cgggtccgaa caggcggcgc ggagcgcgtc gcgggtgacc ggctccccgg
     9781 gtcgctcacg cgccaggacg agcaggttcc ggagccgcac cccgggcagg ggatcgcggc
     9841 gggcgttgtc ggccaggcgg cgcgcgacgt ccaggttttg cgtgagccgc tggccgatgt
     9901 cgagcaggag aggcaggagg cgggggagcg ccgctttcga tgcctgggcg cagaccgcgc
     9961 cgtcgcggat ctcgacctcg ccctcggcgt tcgccgcga
//
LOCUS       DASPJI010000503         4689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_201162, whole genome shotgun sequence.
ACCESSION   DASPJI010000503 DASPJI010000000
VERSION     DASPJI010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201162"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4689)
                     /locus_tag="VEL74_13665"
     CDS             complement(<1..>4689)
                     /locus_tag="VEL74_13665"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63623.1"
                     /translation="LTLHHIAGDGWSMGVLVREVAVLYAAFAEGNPSPLPELPVQYAD
                     FALWQHTWLRGEVLEGEIAWWRQRLAGLPPLLELPTDRPRPAVQSLRGAVRPVMLPAG
                     VLRSSVTLGRSEGATLFMVLLAGLQALLARHSGQRDLAVGTPVAGRNPVEVEGLIGFF
                     VNTLVLRGDLRGDLEGEPTFRELLGRARETALAAHTHQDLPFEKLVQELAPERSLAHS
                     PLFQVLFSLQNAPAARLEIPGLRLRPLRAAAATARFDLTVSVEEEDGELRGTAEYATD
                     LFDATTIDRLLGHWERLLAAAVATPDLPVSALSLLSGAERGQILLEWNDTGPVPVPEV
                     CLHELFADQARRTPEAVALVDGTREVLYRELSEAAGRLAALLRRRGAGPEAVAGVCLE
                     RSADLVIALLAVLEAGAAYLPLDPRLPPARLAALLAGARAALVVSDRRISAGLPWSGP
                     LLLVDEEGDGAPVPGEAGVSPENLAYVLFTSGSTGEPKGVAITHRSAVALVRWAGTVY
                     TPEELAGVLASTALSFDLSVFEIFVPLCLGGTVILARNVLELPSSVPAAGRVTLINTV
                     PSAMAELLRAGGLGASVRTVNLAGEPLPLSLAAQIHATGTVERLWNLYGPSEDTTYST
                     CTQVSRDGSGAPAIGRPVTATRAYVLAGLEPVPVGVAGELYLGGAGLARGYLHRPDLI
                     AERFLPDPFAVTPGGRLYRTGDLARWTAAGELDFLGRADHQVKVRGFRIELGEIEAAL
                     AALDGVREAAVTVWEDPAGDQRLVAYVAGEATAGELRESLRERLPDYMVPAVFVPLET
                     LPRTPNGKVDRKSLPAPEPRHPAEGDLAPRTTVEEVLAGIWAELLGLPAVGVWDDFFA
                     LGGHSLLAARVASRIRRAFGVEIPLRRLFELRTVADLAPEIAAAGPAAEMPALTPQLR
                     EGDPPLSFAQLRLWFLAQLDPAGAAYNMPSLFQLRGALDLPRLETALGEVARRHEILR
                     TSFAERDGMPVQRIAPPRPWMVPVVDLTALPSAWREPEARRLAHQEAWRPFDLSRLPL
                     LRTLLLRLFGEEHAFLLAAHHIVTDGWSMGILVREVSALYGAPGAGSLPELPVQYADY
                     ALWQQRWLQGEPLERQLAFWHRQLAGVPPVPDLATDRPRPPLQTFRGALVPFALPVDL
                     LERLHRIARQESATLFMVLLASLEALLFRITGQEDFCVGSPVAGRVQLETEPLIGLFV
                     NTLVLRADLSGEPRSDALLARVRETVLAAQAHQEVPFEKLVETLQPERNLSHSPLFQV
                     MLAFQNATGRAAELPGLSLRPLRLDSVVSKFDLNLILQESGEGVEGGLEYDSGIFEGP
                     TALRLLAQLRTLLDGIAADPFAAVVDLPLLPQPERHQLLVEWNDTAAEHGELPLIHEL
                     FEEWARRTPGATAAVCGEESLTYGELAERAHRLAWHLIRLGVGPGSLVGIHLRRSLDM
                     IPALLAVLEAGAAYVPLEIGHPPARLRWIVGTLDISCVLTESAQQGVLPAVPDVIYLD
                     RVDLERYRPAAPARRGAPDDLGYIIFTSGSTGTPKGVMVRHRPVVNLLRWAHRTFGFS
                     PADRVLFVTSL"
BASE COUNT          718 a         1695 c         1739 g          537 t
ORIGIN      
        1 agggaggtga cgaagagcac ccggtcggcc ggcgagaagc cgaaggtccg gtgcgcccag
       61 cggagcaggt tgaccaccgg ccggtggcgg accatcaccc ccttcggcgt gcccgtggag
      121 cccgaggtga agatgatgta gcccaggtcg tccggcgccc cccggcgcgc cggtgccgcc
      181 ggccggtacc gctccaggtc gacacgatcc agatagatga cgtcgggcac ggcagggagg
      241 actccctgct gcgccgactc ggtgagcacg caggagatgt ccagcgtgcc gacgatccag
      301 cgcagccgcg cgggcggatg gccgatctcc agcggcacgt aggcggcgcc cgcctcgagc
      361 acggccagga gggccgggat catgtccagg gagcggcgca ggtggatgcc cactagcgag
      421 cccggaccga cgccgaggcg gatcaggtgc caggccagcc ggtgagcccg ctccgccagc
      481 tcgccgtagg tcagcgactc ttcgccacag acggcggcgg tggcgccggg ggtccgccgg
      541 gcccactcct cgaacagctc gtggatcagg gggagctctc cgtgctccgc ggcggtgtcg
      601 ttccactcca ccagcaactg gtggcgctcg ggctgcggca acagcggcag gtccaccacc
      661 gcggcaaagg gatccgcggc gatcccgtcg aggagggtcc ggagctgcgc cagcaggcgg
      721 agggcggtcg ggccctcgaa gatcccgctg tcgtactcga gcccgccttc gaccccctcg
      781 ccgctctcct ggaggatcag gttgaggtcg aacttggaga cgacgctgtc gagccgcagc
      841 gggcgcagcg acaaccccgg cagctccgcg gcccggccgg tggcgttctg aaaggcgagc
      901 atcacctgga acagcggcga gtggctcagg ttccgctccg gctgcagcgt ctccaccagc
      961 ttctcgaagg ggacctcctg gtgggcttgc gccgccagga cggtctcgcg cacccgggcc
     1021 agcagcgcgt cggaccgggg ctcacccgac aagtcggcgc ggagcaccag ggtgttcacg
     1081 aacaggccga tgagcggctc ggtctccagt tggacacgcc cggccacggg cgagccgacg
     1141 cagaagtcct cctggccggt gatacggaag agcagcgctt ccagagaggc gaggaggacc
     1201 atgaacaggg tcgccgactc ctgccgcgcg atccggtgga gcctctccag caggtctacg
     1261 gggagcgcga aggggaccag ggcaccgcgg aatgtctgga gcggcgggcg cggacggtcg
     1321 gtggcgaggt ccggcacggg cggcacgccc gcgagttggc gatgccagaa cgcgagctgc
     1381 cgctccagcg gctcgccctg caaccagcgc tgctgccaaa gggcgtagtc ggcgtactgg
     1441 accggcagct ccggcagcga cccggccccg ggagccccgt acagggcgga gacctcgcgc
     1501 accaggatcc ccatggacca gccgtccgtg acgatgtgat gggccgcgag gaggaaggcg
     1561 tgctcctcgc cgaacagccg cagcagcagg gtgcgcagca gaggcagacg ggagaggtcg
     1621 aaggggcgcc acgcctcctg gtgagcgagg cgccgcgctt cgggctctcg ccaggccgaa
     1681 gggagcgccg tgagatcgac caccggcacc atccagggcc ggggaggcgc gatccgttgg
     1741 accggcatgc cgtcgcgctc ggcgaaggag gtgcgcagga tctcgtggcg gcgggccacc
     1801 tcgcccaggg cggtctccag acgggggagg tcgagcgctc cccggagctg gaagaggctc
     1861 ggcatgttgt acgccgcgcc ggcggggtca agctgcgcca ggaaccagag gcggagctgg
     1921 gcgaaggaga gcggcggatc gccctcccgg agctggggcg tcagggcagg catctcggcc
     1981 gcgggtccgg cggcggcgat ctccggcgcc aggtcggcca cagtccgcag ctcgaagagc
     2041 cgccgcaacg ggatctcgac tccgaaggcc cgccggatgc gcgaggccac ccgcgccgcc
     2101 aggagcgaat ggcctcccag ggcgaagaag tcgtcccaca cgcccacggc cgggaggccc
     2161 agcagctcgg cccagatgcc ggcgaggacc tcctccaccg tcgtacgtgg cgccaggtcc
     2221 ccctccgccg ggtggcgcgg ctccggcgcc ggcagggact tccggtccac cttgccgttg
     2281 ggcgtgcggg ggagcgtttc gaggggtacg aagacggccg gcaccatgta gtccggcagc
     2341 cgctcccgca gggactccct cagctccccg gccgtggcct cgccagccac atacgcgacc
     2401 aatcgttggt cgcctgccgg gtcctcccac acggtcaccg ccgcctcgcg gactccgtcc
     2461 agggcggcca gagcggcctc gatctccccc agctcgatcc ggaagccgcg gaccttgacc
     2521 tggtggtcgg cgcggccgag gaagtcgagc tctcccgcgg ccgtccaccg cgccagatcg
     2581 ccggtgcggt acagccggcc tccgggcgtg accgcgaacg gatcggggag gaagcgctcg
     2641 gcgatcaggt cgggacggtg caagtatccc cgcgcgaggc ccgcaccacc caggtagagc
     2701 tctccggcga cgcccaccgg caccggctcg agaccggcca ggacgtacgc cctggtggcc
     2761 gtcaccggcc ggccgatggc cggagccccg gacccgtccc gggacacctg ggtgcaggtc
     2821 gagtaggtcg tgtcctcgga ggggccgtag aggttccaca gcctctcgac ggtgccggtc
     2881 gcgtggatct gggccgccag ggagagcggc agcggctctc ccgccaggtt gaccgtgcgg
     2941 accgaggctc cgagcccccc cgccctcagg agctcggcca tcgccgaggg gacggtgttg
     3001 atcagggtga cccgtcccgc cgcgggcacg gaggacggca gctccaggac gttccgcgcc
     3061 aggatcaccg tcccccccag gcagaggggg acgaagatct cgaagaccga gaggtcgaag
     3121 ctcagcgccg tggaggcgag gacgcccgcc agctcctccg gcgtgtacac cgtaccggcc
     3181 cagcgcacca gcgccaccgc gctgcgatgg gtgatggcga cccccttggg ctcccccgtc
     3241 gagccggagg tgaagagcac gtaggcgagg ttctcggggc tgacgccggc ctctcccggc
     3301 accggcgccc cgtcaccctc ctcgtccacc agcagcagcg gcccgctcca ggggagcccc
     3361 gccgagatcc tccggtcgct gaccaccagc gcggcccgcg cgccggcgag cagggcggcg
     3421 aggcgcgccg gcggcaaccg cggatccagc ggcagatagg ccgcgccggc ctcgaggacc
     3481 gcgagcaggg cgatgaccag gtccgcagag cgctccaggc agacgccggc caccgcctcc
     3541 gggccggcgc ccctccgccg cagcaacgcc gccaaccggc cggcggcctc agagagctcg
     3601 cggtagagga cctcgcgcgt accgtccacc agggccaccg cctccggcgt ccgccgcgcc
     3661 tggtccgcga acagctcgtg cagacagacc tccggcacgg gcactggccc ggtgtcattc
     3721 cactcgagca ggatctgccc gcgctcggcg ccgctcagga gcgacagggc ggagacgggc
     3781 aggtccggcg tggcgaccgc cgcggcgagc agccgctccc agtgcccgag cagccggtcg
     3841 atggtcgtgg cgtcgaacag gtccgtggcg tactcggcgg tgccgcggag ctccccgtcc
     3901 tcctcctcga cgctgaccgt gaggtcgaac cgggccgtcg ccgccgctgc ccgcagtggc
     3961 ctcaggcgca gccccgggat ctccaggcgt gcggccggag cgttctgcag ggagaacagg
     4021 acctggaaca gcggcgagtg ggcgaggctc cgctccggcg ccagctcctg gacgagcttc
     4081 tcgaacggca ggtcctggtg cgtatgggca gccagcgcgg tctcgcgcgc ccggccgagc
     4141 agctcccgaa acgtgggctc tccttccaga tcgccacgca gatcgccacg caggaccagg
     4201 gtgttgacga agaagccgat gagcccctcg acctccaccg ggttgcgccc ggcgacgggg
     4261 gtgcccaccg cgaggtcccg ctggccgctg tggcgcgcca gcagcgcctg gagcccggcg
     4321 aggaggacca tgaagagcgt tgctccctcg ctccggccca gggtgaccga cgatcgcagg
     4381 accccggccg gcagcatcac cggccggacg gcgccgcgga ggctctgcac cgccggccgc
     4441 gggcggtcgg tggggagctc gaggaggggc ggcaggcccg cgagccgctg gcgccaccag
     4501 gcgatctcac cctccagcac ctcaccgcgc agccaggtat gctgccagag ggcgaaatcc
     4561 gcgtactgca ccggcagctc gggcagcggt gacgggttgc cctcggcgaa agccgcgtag
     4621 agcacggcga cctcacggac caggacgccc atggaccagc cgtcgccggc gatgtggtgc
     4681 agggtgagg
//
LOCUS       DASPJI010000504        10705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_90748, whole genome shotgun sequence.
ACCESSION   DASPJI010000504 DASPJI010000000
VERSION     DASPJI010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10705)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10705
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90748"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>10705
                     /locus_tag="VEL74_13670"
     CDS             <1..>10705
                     /locus_tag="VEL74_13670"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63624.1"
                     /translation="PALPPARLALLVEDACRGLDRPVLVAQGGLEEVLAGLPLAGVRV
                     VAMDDAEEAGEQESVDLPGPASLAYLIYTSGTTGVPKAVMVEHGSLSHTLWAVRTAFS
                     FTADDRMPCLAPFPFDIFLFELLGPLLAGGTAVLYDLRPAPDVPRLARELSGMTLLHS
                     VPALMRQVVEELERQGSRPGIRRVFVGGDAVPGDLLAAMRQTFPQAVVTVLYGPTEGT
                     IIAAHHPVTGTEPPARLLIGRPLPGAGLELRDRDGHLVPIGIPGEIWLAGRGVTRGYL
                     HRPELTAEKYVAMDGRRLYRSGDLGRLLPDGTVEFLGRVDQQVKVRGFRVELGEIEAV
                     LAGHPAMREAVVLARRGTEGGGDRLVAFVVPRQLPADGLVGELRELLQARLPEYMVPA
                     AWVVLESVPLTPHGKVDRRALAARVDEAGMEESGGGTKAWAAPRTATEELLAGIWAEL
                     LGPGRDLRVGRHDDFFDLGGHSLLATRLVTRMGSAFGVEVPLRAVFEAPTVEALARKV
                     EEALAGSSGSQIPPIQPVSRDGVLPLSFTQESLWFLHRLDPQQSLYNVPFPVRLEGRL
                     DVAVLRRSLDEITRRHEVLRTTFPAAAGEPRQEIGPFLPRPLPVIDLAALPEEMRRPE
                     AWRVLTAEGRRPFDLARGPLLDTLLLRAGEHEAILLFNVHHIVSDGWSMGIVTAELAA
                     IYGAFVRGLPLSHAALPELPIQYADFAAWQRQWLSGKVLARQTAYWRRELAGAPTLLA
                     LPTARPQSARPVRPSPEAGILEIELPAELTGTLRVLAQTSGATLFMTLLAAFQTLLHR
                     YTGQEDVLVGSPVANRTRPEIQDLIGLFINTLVLRGRFSGEERGEPPVSCRRLMERTR
                     QAALGAYAHQDLPFERLVEELRIERDLAYNPLVQATFSLQVGDALPSRHLPGLRVAAM
                     RWPDSAAKFDLSLVIEEAGDRLRGELIYRAGLFDHAMARRMAGHLRALLEGMAADPDR
                     PVSELPLLPEVERRELLLAGAGRPQAEACLHERFLEQAARTPGAVALEDETGARLTYG
                     ELAGRAGRLAHVLRRQGVGLETPVGVFLERSPDLVTALLGTLLAGGVYLPLDPAHPPA
                     RTAALLADAGAPVVLTSRGLRDRLPPSGRLVVAVDEIEAGQDDPAAGPALPGSAACLL
                     YTSGSTGTPKAVAVEHRMALQHMETAAELLGIATGDRVLQFAAATFDTSLEQIWTALL
                     RGATLVLRGSALWPPAGLLARVADLGLTVINLPTAYWQQWVHDVAGLAAPPAGLRLRL
                     VLVGGEAMTAEAARLWLAGPLGAVKVINGYGPTEAVVTATAVALESGALPEVGIVEIG
                     RPLPGRNACVLDRNGELAPAGVAGELCLGGILARGYPGRADLTAERFVPDPFATVQGG
                     RVEPGARLYRTGDLARCRPDGSLEFMGRVDRQVKLRGFRIEPGEIEAVLASHPEVREA
                     AVLPLGGPAGTRLVAFVVPRQMAGDGLASEVRKLARSRLPEPLVPSAWIVRESLPLTP
                     HGKVDRKALASFAESLETPGAEGHVPPRTQVEEVLAEIWSDLLGRERVGVHDGFFELG
                     GHSLLATRMVARVEEALSVELPLQVVFEAATLAKLASRIEAVVAGEAEMTAPPIRPVP
                     LKERGVLPLSFAQERLWFLDRLEPNRALYNVPFAMRLTGPLDVASLNRTLDEIVRRHE
                     ALRTTFPDAAGEPRQEVAPFVHRPLPVADLTALPEDVREAESDRWLAEEATRSFDLAC
                     GPLLRTLLLRLDADKAVLLLNTHHIVSDGWSMGVMTSELAALYDAYFRGLSSPLPELA
                     VQYADFAVWQRQWLSGKVLERQIAYWRQELAGAPDLLELPTDRPRTVNQTRKGGEWPV
                     SLPAGLARDVRALGRGQGATLFMTLLAAYQTLLSRLTGQDDLLVGMPIANRNRAETEG
                     LIGFFANTLALRGKPAGERSFRSLLVQVRDTALGAYSYQDVPFERLVEELRVKRSLDH
                     NPLVQAVFALQNAFTTLAQPAGLSLSHVLLPLNAAKFDLSLSLEETGGEIAGLFEHSA
                     DLLDDATVGRWAGHFRELLAGAVAEPDRPLSSLPMLSAAERRQLAEWNDTAVPEGEKP
                     ADVPVHRLFEAQAERTPGDVAVVFGGEELTYAELDRRAGRLAGRLRALGVGAEARVGL
                     LLRRSVDLVAAILAVWKAGGAYVPLDPGLPQARLAMMVEDAFEDLERPVLLTQKGLEE
                     VAAGLSLGEARVLLVDGEEEPATEAAGASSDGEIGRLAYLIYTSGTTGRPKAVMVEHG
                     QLAHTLRAARLAFGFAPGDRMPCLAAFSFDIFLFELLGPLLAGGTAVLYDLRPAPDVP
                     GLARDLSSMTLMHAVPALMRQVVEELDRQGSRPGLRRVFVGGDAVPGDLLAAMRQAFP
                     AARITILYGPTEAAIIAARHEVTGSEPASRLLIGRPLPDVTLDLRDRSGNLVPVGVPG
                     EIWLGGPGVTRGYLHRPELTAAVFAAEEGRRFYRTGDLSRRAVDGTVEFLGRIDQQVK
                     VRGFRVELGEIEAALAAAPGVRQAVVLVREWRAGRSEGAAGSPGDRRLVAYVAGAAGD
                     ISVEELSRSLRGRLPEYMVPSAWVVLEALPLTSHGKVDRRALAAIEPVEEGGEPEAGG
                     APRTPAEELLAGLWAEILGRARIGIHDDFFEAGGHSLLATRVISRLRDAFGVDLPLRV
                     LFEAPTVASLAARVEVALAEGRGLQAPPIRPVPRGDGTDLPLSFTQERFWFLDQLEPG
                     SPLYTMAGVLRLTGLPDVPALERAFNEIVRRHEALRTTFEGREGKPFQRVHARLWVPL
                     PLMDLRSLAAGSRDAEVQRVIKDEERQPFDLSTGPLLRLGLLRLEESSWILLFTLHHI
                     VSDAWSSGLLIRELATLYGAFSRGLPPERPVLPELPVQYADFAVWQREWLAGEVLAEQ
                     VGYWTRALEGAPTLVELPSDRPRLPGRSLRSAVRRLDLPDELATGLQALSRRGGATLF
                     MTLLAGFDALLFRHTGQADLLVGTPIANRNRGEIEGLIGCFINTLVLRGRMDDGTLSF
                     SELLERVKSTTLEAYVHQDLPLEKVVEALQVERTATHNPLFQVMFALQNAPLGELALP
                     GLTLTPVETESELVTNFDLSLGLTDIDGGLWGGCIYRLDLFDEATVDRLLERFRAILE
                     AVVADPARPLLELPGWLEEHRAQIAAAERNAAGMAAATAHPQGEKEEDPEEAARTRLA
                     AREEKVAEKRSALSDKKKAALAKLLKGKQAAAPAASGSQGGAPSPAPAPAFPRRPDPS
                     SAVLSFAQERLWFLDRLEPGTALYNIAIPLRLNGRLDPRAAERSLAELVRRHEVLRTT
                     FAGEGGEARQIIGPAIPPPLPVVDLRGLPAAAREAELQRQVRDESLRPFDLERGPLLR
                     AFLLRLDAEVWAGVFNLHHIVSDAWSSGILVREFGTLYSAFAEGRAPALPELPIQYGD
                     FAHWQRQWLSGEVLEAQLSYWERELADAPPLLELATDRPRSSRPGSAGAVESVDLRAG
                     LAPSLRALSLSANATLFMTVLAAFQVLLHRYTAQDDVLVGSAIANRTRPEVQALIGFF
                     VNTLVLRGRCAPEPALSFRRLLDRTREAALGAYAHQDLPFERLV"
BASE COUNT         1312 a         3808 c         3995 g         1590 t
ORIGIN      
        1 acccggcgct gccgccggca cggctggcgc tgctggtgga ggacgcctgc cgcggcctcg
       61 accgtccggt gctggtggcg cagggcggcc tggaggaggt cctggccgga ttgccgctgg
      121 ccggcgtccg tgtcgtcgcc atggatgacg cggaggaggc cggcgagcag gaatccgtgg
      181 atctcccggg gccggcgagc ctggcctatc tcatctacac ctcggggacc accggcgtcc
      241 ccaaggcggt catggtggag cacggcagtc tctcccacac cctgtgggcg gtgcgcaccg
      301 cgttctcctt cacggcggac gaccgcatgc cctgcctggc gccgttcccg ttcgacatct
      361 tcctcttcga gctgctgggg ccgctgctcg ccggcggcac cgcagtgctc tacgacctgc
      421 gtccggcgcc ggacgtgccg cgcctcgccc gcgagctctc gggcatgacg ctgctgcact
      481 ccgtgccggc cctgatgcgg caggtggtgg aggagctgga gcggcagggg agccggccgg
      541 ggatacggcg ggtcttcgtc ggcggcgacg cggtgccggg ggatctgctg gccgccatgc
      601 ggcagacctt cccgcaagcc gtcgtcaccg tgctctacgg cccgaccgag gggacgatca
      661 tcgccgcgca tcatccagtg accgggacgg agcctccggc gcggctgctc atcggccggc
      721 cgctcccggg cgccggtctg gagctgcgcg accgcgacgg ccacttggtc ccgatcggca
      781 tcccggggga gatctggctc gccggacggg gtgtgacccg gggctacctc caccggccgg
      841 agctgaccgc cgagaaatac gtggcgatgg acggccgccg cctctaccgc agcggcgacc
      901 tgggccggct cttgccggac ggcacggtcg agttcctggg tcgggtcgac cagcaggtca
      961 aggtgcgcgg cttccgggtt gagctgggag agatcgaggc ggtcctggcc ggtcacccgg
     1021 ccatgcggga ggcggtggtg ctggcccggc ggggcaccga aggtggcggc gaccggctcg
     1081 tggccttcgt ggtgccgcgc cagctccccg cggacgggct ggttggggag ctgcgggagc
     1141 tgctccaggc gcggctgccg gagtacatgg tgcccgccgc gtgggtggtg ctggagtcgg
     1201 tgccgctgac gccccacggc aaggtggacc ggcgagcgct ggcggcccgc gtggacgagg
     1261 ccgggatgga ggagagcggc ggcgggacga aggcctgggc cgcgccgcgc actgccacag
     1321 aggagctgct ggcggggatc tgggccgagc tcctagggcc gggccgggat ctccgcgtcg
     1381 gccgccacga cgacttcttc gatctcggcg ggcactccct gctggcgacg cggctggtga
     1441 cccggatggg gagcgccttt ggcgtcgagg tccccctgcg ggcggtgttc gaggcgccga
     1501 cggtcgaggc tctggcgagg aaggtggaag aggctctcgc cggctctagc ggcagccaga
     1561 tcccgccgat ccagccggtc tcccgtgacg gggtgctgcc gctctccttc acgcaggaga
     1621 gcctctggtt cctccaccgt ctggacccgc agcagtcgct ctacaacgtc cccttccccg
     1681 tgcggctgga agggcgcctt gacgtcgccg tcctgcggcg gagcctggac gagatcacgc
     1741 gacgccacga agtgctgcgc accaccttcc ccgccgccgc gggcgagccg cggcaggaga
     1801 tcggcccgtt ccttccccgg ccgctgccgg tgatcgatct ggcggcgctg ccggaggaga
     1861 tgcgccggcc cgaggcgtgg cgggtgctca cggcggaggg acggcggccc ttcgacctcg
     1921 ctcgcgggcc actcctcgat acgctgctgc tgcgggcagg ggagcacgag gcgatcctgc
     1981 tcttcaacgt acaccacatc gtgagcgacg gctggtccat gggaatcgtg accgcggagc
     2041 tggccgcgat ctacggtgct ttcgtccggg ggctgccatt gtcgcacgca gcgttgccgg
     2101 agctgccgat ccagtacgcc gacttcgcgg cgtggcagcg gcagtggctg tcgggaaagg
     2161 tgctggcacg gcagaccgcc tactggcggc gggagctggc gggagcgccc accctgctgg
     2221 cgctgcctac ggcacggccg caatcggcgc ggccggtgcg tccgtccccc gaagcgggca
     2281 tcctggagat cgaactgccg gcggagctga cgggcactct ccgcgtcctc gcccagacct
     2341 cgggcgccac cctgttcatg acgctcctcg ccgccttcca gacgctgctc catcgctaca
     2401 ccgggcagga ggacgtgctc gtgggctcgc ccgtggccaa tcgtacccgg cccgagatcc
     2461 aggatctgat cggcctcttc atcaacaccc tggtgctgcg cgggcgcttt tccggtgagg
     2521 agaggggaga gccccccgtc tcatgtcggc ggctgatgga gcggacgcgg caggccgccc
     2581 tcggggccta cgcgcaccag gacctcccct tcgagcgtct ggtggaggag ctgcggatcg
     2641 agcgggatct cgcgtacaac cccctggtcc aggcgacgtt ctcgctccag gtgggagacg
     2701 ccctgccgtc gcggcacctc ccggggctcc gggtcgcggc gatgcggtgg ccggattccg
     2761 ccgccaagtt cgacctgtcc ctcgtcatag aggaggccgg cgaccggctg aggggggagc
     2821 tgatctaccg ggccggcctg ttcgaccacg caatggcgcg gcggatggcg gggcacctgc
     2881 gggccctgct ggaggggatg gcggccgatc cggaccggcc ggtctcggag ctgccgcttt
     2941 tgccggaggt ggagcggcga gagctgctgc tggctggagc tggccgtccc caggccgagg
     3001 cgtgcctcca cgagcgcttc ctggagcagg cggcgcgcac ccccggtgcc gtggccctgg
     3061 aggacgagac cggagcgcgg ctgacctacg gtgagctggc ggggcgggcc ggccggctgg
     3121 cgcacgtcct gcggcgccag ggggtgggcc tggagactcc ggtcggcgtc ttcctggagc
     3181 gctcgcccga cctggtgacg gcgctcctcg gcacgctcct cgccggtggg gtctacctgc
     3241 ccctcgaccc cgcccatccc ccggcgcgca ccgccgcctt gctggcggac gccggcgcgc
     3301 cggtggtgct gacctcccgc ggtctgcggg accggctgcc cccctccggc cgcctggtgg
     3361 tggccgtaga cgagatcgag gcaggacagg acgatccggc cgccggcccc gcgctgccgg
     3421 ggagcgcggc ctgtctgctc tacacctccg gctccaccgg cacgcccaag gcggtggccg
     3481 tcgagcatcg gatggcgttg cagcacatgg agaccgcggc ggagctgctg gggatcgcga
     3541 cgggagaccg agtgttgcaa ttcgccgccg cgaccttcga cacctcgctg gagcagatct
     3601 ggacggcgct cctccgtggc gccacgctgg tgctgcgcgg ctccgccctc tggccgccgg
     3661 cgggtctcct cgcccgcgtc gccgacctgg gcctgaccgt gatcaatctg ccgaccgcct
     3721 actggcagca gtgggtccac gatgtcgccg gactggcggc cccgccggca gggctgcgcc
     3781 tgcggctggt gctggtcggc ggggaggcca tgacggccga ggcggcgcgc ctctggctgg
     3841 cgggccccct gggcgctgtg aaggtgatca acggctacgg ccccaccgag gcggtggtca
     3901 ccgccaccgc ggtggctctg gagagcggag ccttgccgga agtcggcatc gtcgagatcg
     3961 ggcggccgct gcccgggcgc aacgcctgcg tgctcgaccg gaatggcgag ctggcgccgg
     4021 ccggcgtcgc gggtgagctg tgcctaggag ggatcctcgc ccggggctac ccggggaggg
     4081 ccgatctcac ggcggagcgc ttcgtccccg atccgttcgc caccgtccag gggggccggg
     4141 tggagccggg tgcccgtctc tatcgcaccg gcgacctggc gcgttgccgg cccgacggca
     4201 gcctggagtt catgggccgc gtcgaccgcc aggtaaagct ccggggtttc cgcatcgagc
     4261 ccggggagat cgaggctgtt ctggcctccc atccggaggt acgggaggcg gcggtgctgc
     4321 ccctcggtgg gcccgcaggg acccgcctgg tggctttcgt cgtgccgcgg cagatggcag
     4381 gggacggcct cgcgtctgag gtgcgcaagc tggcccggtc gcggctgccg gagcccctgg
     4441 tgccttcggc atggatcgtc cgcgagtcgc ttcctctgac cccgcacggc aaggtggacc
     4501 ggaaggccct ggcctccttc gcggagtccc tggaaactcc aggagcggaa ggccatgtgc
     4561 cgccacgcac gcaggtggag gaggtgctgg cggagatctg gagcgacctc ctgggccggg
     4621 agcgggtggg cgtacacgat gggttcttcg agctgggcgg ccactcgctg ctggcgactc
     4681 gcatggtcgc gcgggtggag gaggctctga gcgtcgagct gccgctgcag gtggttttcg
     4741 aggccgccac gctggcgaag ctggccagcc ggatcgaggc cgtcgtagcg ggcgaggcgg
     4801 agatgacggc cccgcccatc cgtccggtgc cactgaagga gagaggcgtg ctgcccctct
     4861 cgttcgcgca ggagcgcctc tggttcctcg accgtcttga gccgaaccgg gcgctctaca
     4921 acgtcccctt cgccatgcgc ctgactggcc cgctcgacgt cgcctctctc aacagaaccc
     4981 tggacgagat cgtccggcgt cacgaggcgc tgcgcaccac cttcccggat gcggccggcg
     5041 agccgcgcca ggaggtcgcg cctttcgttc accgtcctct gccggtcgcc gatctcaccg
     5101 cgctgccgga ggacgtccgg gaggccgaga gcgaccggtg gctcgccgag gaggcgacgc
     5161 ggtcgttcga cctggcgtgc gggccgctgc tgagaaccct gctgctgcgg ctggacgcgg
     5221 acaaggccgt gctgctgctc aacacgcacc acatcgtgag cgacggctgg tccatggggg
     5281 tgatgaccag cgagctggcg gcgctctatg acgcctactt ccgggggctc tcctcgcccc
     5341 tgccggagct ggcggtccag tacgccgact tcgcggtctg gcagcggcag tggctgtccg
     5401 gcaaggtgct ggagcggcag atcgcctact ggcgtcagga gctggcggga gcgccggacc
     5461 tgctggagct gcccaccgac cggccgcgca ccgtgaacca gacccgcaag gggggagagt
     5521 ggccggtgtc gctccccgcc ggcctggcgc gggacgtccg ggcgcttggc cgcggccagg
     5581 gggcgacgct gttcatgact ctgctggccg cctatcagac gctgctctcc cgactcaccg
     5641 gccaggacga cctgctggtg ggcatgccga tcgccaaccg taaccgggct gagaccgaag
     5701 ggctcatcgg cttcttcgcc aacaccctgg cgttgcgggg gaaaccggcc ggggagcgct
     5761 ccttccgctc gctactggta caggtccgcg acacggccct cggcgcctac tcctatcagg
     5821 atgtgccgtt cgagcgtctg gtcgaggagc tcagggtcaa gaggagcctc gaccacaatc
     5881 ctctggtgca ggcggtcttc gccctccaga acgccttcac caccctggcc cagcccgccg
     5941 ggctgtcgct ctcccacgtg ctcctgcccc tgaacgccgc caagttcgac ctcagcctgt
     6001 cgctggagga gaccggaggg gagatcgccg gcctgttcga gcacagcgcc gacctgctgg
     6061 acgacgccac ggtagggcgc tgggccgggc atttccgcga gctcctcgcg ggtgccgtgg
     6121 cggagccgga ccggcccctc tccagcctgc ccatgctctc cgccgccgag cgccgccaac
     6181 tcgccgaatg gaacgacacg gccgtgccgg agggagagaa gccggcggac gtccccgtgc
     6241 accgcctgtt cgaggcccag gcggagcgca cgcccggcga cgtggcagtg gtcttcggcg
     6301 gcgaggagct gacctacgcc gagctggacc gccgcgccgg gcggctggcc ggccgcctgc
     6361 gcgcgctcgg cgtcggcgcg gaggcgcggg tcggcctgct gctgcgccgg tcggtggacc
     6421 tggtcgccgc catcctcgcc gtctggaaag ccggcggcgc ctacgtcccc ctcgatccgg
     6481 ggctgcccca ggcccgtctg gcgatgatgg tggaggacgc cttcgaggat ctcgagcggc
     6541 cggtgctgct cacccagaag gggttggagg aggtcgcggc cgggctctcc ctcggggagg
     6601 cccgcgtgct cctggtggac ggggaggagg agccggcgac ggaggccgct ggcgccagct
     6661 ctgacggtga gatcgggcgc ctcgcctatc tcatctacac ctccgggacc acaggccggc
     6721 ccaaggcggt catggtggag cacggccagc tcgcccacac gctgcgcgcg gcccgtctcg
     6781 ccttcggctt cgcgcccggt gaccggatgc cgtgcctggc cgcgttctca ttcgacatct
     6841 tcctcttcga gctgctgggg ccgctcctcg ccggcggcac ggcggtgctc tacgacctgc
     6901 ggccggcgcc ggacgtcccc ggtctggccc gcgacctctc cagcatgacg ctgatgcacg
     6961 cggtgccggc cctgatgcgt caggtggtgg aggagctgga ccggcagggg agccggccgg
     7021 gcctgcggcg cgtgttcgtg ggcggcgacg cggtcccggg cgatctgctg gccgccatgc
     7081 gccaggcctt cccagcggcg cgcatcacca tcctctatgg ccccaccgag gcggccatca
     7141 ttgccgcccg gcacgaagtg acggggagcg agccggcgtc gcggctgctc atcggccggc
     7201 cgctgcccga cgtcaccctc gatctgcgcg accggtccgg caacctggtg ccggtgggcg
     7261 taccagggga gatctggctg ggcggtccgg gggtgacccg gggatatctg caccgccccg
     7321 agctgacggc tgccgtcttc gccgccgagg aagggcgccg cttctaccgg accggcgacc
     7381 tgagccggcg ggcggtggac ggcacggtgg agttcctggg ccggatcgac cagcaggtca
     7441 aggtgcgggg cttccgcgtc gagctggggg agatcgaggc ggcgctcgcg gcggcacccg
     7501 gagtccgcca ggcggtggtg ctggtacgcg agtggcgagc cggccgatcg gagggggccg
     7561 ccggatcgcc aggggaccgg cggctggtcg cctatgtggc gggcgccgcc ggcgacatct
     7621 cggtcgagga gctgagccgg tcgctgcgcg ggcggctgcc ggagtacatg gtgccctcgg
     7681 cctgggtggt gctggaggcc ctgccgctca ccagccacgg caaggtggac cgccgcgcgc
     7741 tcgccgccat cgagcccgtg gaggagggcg gcgagccgga ggccggaggc gcgccgcgta
     7801 cgccggccga ggagctgctc gcggggctct gggcggagat cctcggccgc gcccggatcg
     7861 gcatccacga cgatttcttc gaggccggcg gccactcctt gcttgccacc cgcgtcatct
     7921 cccgcctgcg ggacgcgttc ggcgtcgacc tgccgctgcg cgtcctcttc gaggccccta
     7981 ccgtcgcgtc gctggcggca cgtgtcgagg tcgccctggc cgaggggcga gggcttcagg
     8041 cgccgccgat ccggccggtt ccgcgcggag acgggacgga cctgccgctc tcgttcactc
     8101 aggagcgctt ctggttcctc gaccagctcg agcccgggag cccgctctac accatggccg
     8161 gcgtcctgcg gctcaccggc ctgcccgacg tgccggcgct ggagcgcgcc ttcaacgaga
     8221 tcgtccgccg ccacgaggcg ctacgcacca ccttcgaagg ccgggaagga aagcccttcc
     8281 agcgcgtcca cgcgcgcctg tgggtgccgt tgccgctcat ggatctgcgg tcgctggccg
     8341 cgggctcccg cgacgcggag gtccagcgcg tcatcaagga cgaggagcgg cagcccttcg
     8401 acctctcgac ggggccactc ctgcgcctcg gcctgctgcg gctggaggag agctcctgga
     8461 ttctcctctt caccctccac cacatcgtca gcgatgcctg gtcgtcaggg ctgctgatcc
     8521 gcgagcttgc gacgctctac ggcgccttct cgcgcgggtt gccgccggag cgaccggtgt
     8581 tgccggagct gccggtgcag tacgccgact tcgcggtctg gcagcgagag tggctggccg
     8641 gcgaggtgtt ggccgagcag gtcggctact ggacccgggc cctggagggc gcccccacgt
     8701 tggtggagct gccgagcgac cggccgcggc tgccggggcg gagcctgcgc tccgccgtgc
     8761 ggcggctgga tctgccggac gagctggcaa cggggctcca ggccctgtcc cggcgcggcg
     8821 gcgccaccct gttcatgacc ctgctggccg gcttcgacgc cctgctcttc cggcacaccg
     8881 ggcaggcgga cctgctggtg ggcaccccca ttgccaaccg caaccgcggc gagatcgagg
     8941 ggctgatcgg ctgcttcatc aacaccctgg tactgcgcgg ccggatggac gacgggactc
     9001 tctcgttcag cgagctgctg gagcgcgtca agagcaccac cctggaagcc tacgtccacc
     9061 aggatctgcc gctggagaag gtggtggagg cgctgcaggt ggagcgcacg gccacccaca
     9121 accccctgtt ccaggtgatg ttcgccctgc agaacgcccc gctgggagag ctggcgctgc
     9181 cgggcctcac cctcaccccc gtcgagaccg agtcggagct ggtgacgaac ttcgacctga
     9241 gcctggggct caccgatatc gacggtggcc tttggggcgg ctgcatttac cggctcgatc
     9301 tgttcgacga ggcgacggtg gaccggctgc tggagcgctt ccgcgccatc ctggaggcgg
     9361 tggtggccga tccggcccgg ccgctgctcg agttgccggg gtggctggag gagcaccggg
     9421 cccagatcgc cgccgccgaa cggaatgccg ccgggatggc cgctgcgacg gcccatcccc
     9481 agggagagaa ggaggaggac ccggaggagg cggcccgcac ccggcttgcc gcccgggagg
     9541 agaaggtggc ggagaagcgc tccgccctct ccgacaagaa gaaggcggct ctcgccaagc
     9601 tcctcaaggg caagcaggcg gcggccccgg cggcttcagg cagccagggg ggcgctccgt
     9661 cccccgcgcc ggcgcccgcg ttcccgcggc ggcccgaccc ctccagcgcg gtcctctcct
     9721 tcgcccagga gcggctctgg ttcctcgacc ggctggagcc ggggacggcg ctctacaaca
     9781 tcgccatccc tctgcgcctc aatggccggc tcgatccgcg ggcggcggag cggagcctcg
     9841 ccgagctggt gcgccgccac gaggtgctgc gcaccacctt cgccggcgag ggaggcgagg
     9901 cgcggcagat catcggaccg gcgatcccgc cgccgctgcc agtcgtcgac ctgcggggcc
     9961 tgccggcggc ggcccgcgag gccgagctgc aacgccaggt gcgggacgag tcgctgcgcc
    10021 cgttcgacct cgaacggggt cccctgctgc gcgccttcct gctgcggctg gacgccgagg
    10081 tctgggccgg ggtgttcaac ctgcaccaca tcgtgagcga cgcctggtcg tcaggcatcc
    10141 tcgtccgcga gttcggcacg ctctacagcg ccttcgccga ggggagagcc cccgcgctgc
    10201 cggagctgcc catccagtac ggcgacttcg cgcactggca gcggcagtgg ctctccggcg
    10261 aggtgctgga ggcccagctc tcctactggg agcgagagct ggcggacgcg ccgccgcttc
    10321 tggagctggc gaccgacaga ccgcggtcgt cgcgtcccgg atcggcgggc gcggtcgaga
    10381 gcgtcgatct gcgggcgggc ctggcccctt cccttcgcgc cctgtccctg tcggccaacg
    10441 ccacgctgtt catgacggtg ctcgccgcct tccaggtgct gctgcaccgt tacacggcgc
    10501 aggacgacgt gctggtgggc tcggcgatcg ccaaccgcac ccggcccgag gtccaggccc
    10561 tcatcggctt cttcgtcaac accctggtgc tgcgcgggcg gtgcgcgccg gagcccgcgc
    10621 tgtcgttccg ccggctgctg gaccggaccc gcgaggccgc actgggcgcc tatgcccacc
    10681 aggacctgcc cttcgagcgc ctggt
//
LOCUS       DASPJI010000505         2962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_599840, whole genome shotgun sequence.
ACCESSION   DASPJI010000505 DASPJI010000000
VERSION     DASPJI010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599840"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1573)
                     /locus_tag="VEL74_13675"
     CDS             complement(<1..1573)
                     /locus_tag="VEL74_13675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HYG63625.1"
                     /translation="MNIALVVMPFAAAERPSLGVGLLQSLLRRRGFGCDAKYFNLTFA
                     SLIGLEDYKRFAEEFPIGALAGEWVFSQAFYGRELSDWDSYAEEVLSHPLWGISRGSW
                     PVLRRVLAEAPRFLRLVYESNDWSRYDLVGFTSTFDQTMPSMCLARMIREHHPGVLLA
                     AGGANFEATMGRGYMDLFPFLDYVCTGEGDTAFPNLMDNLRRGSPEVPPGILSRSRPA
                     VGAGSPPPVTNLDALPVPDYDDYHRAASRMPALAGMAYLPLETSRGCWWGAKMHCTFC
                     GLNGETMAFRRKHWRRVADEVAALEERYRPRVLQFTDNILGMGYFEDLLPHWAAAGGT
                     TPKFFEIKANLSRPQVRLLRDSGILYVQPGIESFSDRTLKVMGKGVSAFQNVAVLRWC
                     QELGIDSQWNLLFGFPGEDVAEYPAHAELLRRLVHLRPPEACAPIRLDRFSPNFTRYR
                     EQGFTSIRPMPSYRHVFPLDEDGLMEIAYFFEYTHLHRDRVRELGAPLVRLGRDWHRR
                     WKRGENGTLAVRPHVL"
     gene            1745..2335
                     /locus_tag="VEL74_13680"
     CDS             1745..2335
                     /locus_tag="VEL74_13680"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HYG63626.1"
                     /translation="MKKPTAPRTRCFWVSDDPLYISYHDDEWGVPVHDERRLFEMLIL
                     EGAQAGLSWITILRRREGYRQAFDGFDPKVIARYGPAEIAARLTDPGIVRNRAKVEAT
                     VQNARTLLELRDQGESLDGLLWSFVGGRPRQNRWSGRGEVPAETAESKAMSKELRRRG
                     FTFVGPTICYAFMQAVGMVNDHTVDCFRHHEVGRLA"
     gene            2417..>2962
                     /locus_tag="VEL74_13685"
     CDS             2417..>2962
                     /locus_tag="VEL74_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HYG63627.1"
                     /translation="MKSSRTITRCLLLAAALLGSPAGSFAQVFPAPGEPVDLQMVSRI
                     RDEGLHRSKVMETVEHLTDVIGPRLTGSPQMKAANDWTRDKLAEWGLANARLEPWPFG
                     RGWTYSRVAVHMVKPHQVPLFSLPKGWTPGTNGPVRAPVVRVDIQSEKDFETYRGKLA
                     GKIVLRDPVRDVTDRMPVDPRR"
BASE COUNT          479 a          997 c         1063 g          423 t
ORIGIN      
        1 ccagcacgtg aggccggacc gcgagcgtcc cattctcccc ccgcttccat cgccggtgcc
       61 agtcgcggcc gaggcgcacc agcggcgcgc cgagctcccg cacccggtcg cgatggaggt
      121 gcgtgtactc gaagaagtag gcgatctcca tcagcccgtc ctcgtccagt gggaagacgt
      181 gacggtagga tggcatgggg cggatcgagg tgaagccctg ctcgcggtag cgggtgaagt
      241 tggggctgaa gcggtccagg cggatcggcg cgcaggcctc cggcggccgc aggtggacga
      301 ggcggcggag cagctccgcg tgcgccgggt actcggcgac gtcctcgccg gggaagccga
      361 agaggaggtt ccactgggaa tcgatgccta gctcctggca ccagcgcagg accgccacgt
      421 tctggaaggc ggagaccccc ttgcccatga ccttcaaggt ccggtcggag aagctctcga
      481 tgccgggctg gacgtagagg atccccgaat cccggagcag ccgcacctgc ggccggctca
      541 ggttggcctt gatctcgaag aacttcggcg tcgtgccgcc ggcggccgcc cagtgcggca
      601 ggaggtcctc gaagtacccc atgccgagga tgttatcggt gaactgcagg actcggggcc
      661 ggtagcgctc ctcgagcgcc gccacctcgt cggccacccg gcgccagtgc ttgcgccgga
      721 acgccatggt ctcgccgttc aggccgcaga aggtgcagtg catcttggcc ccccaccagc
      781 agccgcgcga ggtctcgagg gggaggtagg ccataccggc gagcgcgggc atgcgggagg
      841 ccgcccggtg atagtcgtcg tagtcgggca ctggcagggc atcgaggttc gtgaccgggg
      901 gcggcgagcc ggcaccaact gccggccggc tacgggacag aatgcccggc ggcacctcgg
      961 ggctgccgcg gcgcaggttg tccatcaggt tggggaaggc ggtgtccccc tcgccggtgc
     1021 agacgtagtc gaggaagggg aacaggtcca tgtagccgcg ccccatcgtc gcctcaaagt
     1081 tggcgccgcc ggcggcgagc aggacccccg ggtggtgctc gcggatcatc cgcgccagac
     1141 acatgctcgg catggtctgg tcgaaggtgc tggtgaagcc gacgaggtcg tagcggctcc
     1201 agtcgttcga ctcgtacacc aggcgcagga accggggggc ctcggcgagc acccggcgca
     1261 gcaccggcca tgagccccgg gagatgcccc agaggggatg ggagagcacc tcctcggcgt
     1321 aggagtccca gtccgagagc tcgcggccgt agaacgcctg cgagaacacc cattcgccgg
     1381 ccagggcacc gatagggaac tcctcggcga accgtttgta gtcctcgagg ccgatcagcg
     1441 aggcgaaggt gaggttgaag tacttggcgt cgcagccgaa gccgcgccgg cgcagcagcg
     1501 actgcaggag acctaccccg aggctcgggc gctcggcggc ggcgaagggc atgacgacga
     1561 gggcgatgtt cacgggtccg cgctccggtc caacgcgccc gcggctgccg gagcctcgat
     1621 cccgagatgg aagcaccggg agccgacgcg ggcagccagg ggattatacg ggcttccagc
     1681 cgagcttccg gaaggcgcgc cctcgtgata caaaggcgcg gctccgagcc tccgagcgtc
     1741 gccgatgaag aagccgaccg ccccccgcac acgctgtttc tgggtttccg acgacccgct
     1801 ctacatctcg taccacgacg acgagtgggg ggtgccggtc cacgacgagc gccggctttt
     1861 cgagatgctg atcctcgagg gagcccaggc cgggctctcc tggatcacca tcctgcggcg
     1921 gcgggaggga tatcggcagg cgttcgacgg cttcgacccg aaggtgatcg cccgctacgg
     1981 tccggcggag atcgccgccc gtctcaccga ccccggcatc gtgcgcaacc gggccaaggt
     2041 ggaggcgacg gtacagaacg cccgaacgct cctcgagctg cgggatcaag gggagagcct
     2101 cgacggcctg ctgtggagct tcgtgggcgg ccgcccgcgg cagaaccgct ggagcggccg
     2161 cggcgaggtg ccggccgaga cggcggagtc caaggcgatg agcaaggagc tgcggcggcg
     2221 cggcttcacc ttcgtggggc ccaccatctg ctatgccttc atgcaggccg tgggcatggt
     2281 caacgaccac acggtagatt gcttcaggca ccacgaggtc ggccggctcg cctgaaaact
     2341 gtccctccgc gcccgtctgg ccacacctga tgtccgatcg catcttttgg gccgctgccg
     2401 gccatggagg tctgtcatga agtcgtcgcg tacgatcacc cgctgccttc tcctcgccgc
     2461 cgccctgctg gggtcaccgg cgggctcctt cgcccaggtc ttcccggcac cgggcgagcc
     2521 ggtggacctc cagatggtca gccgcatccg cgacgagggg ctccaccgct ccaaggtcat
     2581 ggagaccgtg gagcacctga ccgacgtcat cggccctcgc ctcaccggct cgccgcagat
     2641 gaaggcggcc aacgactgga cgcgcgacaa gctggcagag tgggggctcg ccaatgcccg
     2701 cctcgagccc tggccgttcg gccgcggctg gacctacagc cgggtggcgg tgcacatggt
     2761 caagccgcat caggtgcccc tcttctccct gccgaagggg tggacgccgg gcaccaacgg
     2821 cccggtgcgg gcgccggtcg tgcgggtgga catccagtcg gagaaggact tcgagaccta
     2881 tcgcggcaag ctcgccggca agatcgtcct gcgcgatccg gtgcgcgacg tcaccgatcg
     2941 aatgccggtg gatccccggc gc
//
LOCUS       DASPJI010000506        15731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_260186, whole genome shotgun sequence.
ACCESSION   DASPJI010000506 DASPJI010000000
VERSION     DASPJI010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15731)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15731
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260186"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..991)
                     /locus_tag="VEL74_13690"
     CDS             complement(62..991)
                     /locus_tag="VEL74_13690"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYG63628.1"
                     /translation="MPRKLRHVPEGGSLVEVTCRTVQSRLLLAPRTYVRETVLGVLAR
                     AKRLYPVKLICFVFASNHYHLLMEVEDAAEMAAFMGYLNSNLARELGRLADWKEKFWG
                     RRYQHIVVSDEEGAQIARLKYALSHGAKEGLVGSPREWPGAHAVNALLTGNPMEGVWH
                     DRTQEYRYRLRGKEPEPGQFATVETLTLDPLPCWKHLPPEEYRRRIAELVAEIEEEAR
                     VAREAKGIEPLGVDAVRRQQPHTRPNRTKKSPAPLFHTASKKAYRSLREAYSRFVAAF
                     RDAAEKLRAGDRMVAFPEGSFPPAAPFVRPAFA"
     gene            1211..2656
                     /locus_tag="VEL74_13695"
     CDS             1211..2656
                     /locus_tag="VEL74_13695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYG63629.1"
                     /translation="MVRLSHGAIAIQTVILSVFLTVSPATAQEPEGPSGGLAAFAGEE
                     EVRDAIAPAQLAEIRASVAAHERRKGLTAAPGQEEAPFLFPFFPHAGVQGRDLYLSNF
                     TDQDLSSVTVRDWDCSAQAYDGHHGHDSLIRSFREQAIGVPIFAVRDGVVAATHDGEP
                     DMNTVWDPTTKANYVLVDHGNGYLTWYLHMRNGSVAVSPGQTVTAGTQLGLTGSSGLS
                     NWPHLHFETHKDGTWTEPSAGPCRPGDSLWVSQPPVPRDFYVADFYLAQGAILIPDFT
                     TWLHDPATRKATFLKGVQRVGARVDMRSLPAHSTYRIQMLDPRGKVVFQDSGSFENPV
                     PQKLAVATFWFDMNLSPGTWRFRADFNDSPAVDAPFRVVATSRQVRNRRPNAVKTLLT
                     PARPVAGQAMTCEVRAPLIGRDPDYDIVSYRYEWKVNNRVVRSVTSAALTDLLAAGKA
                     KPGDRVSCRVTPSDGKALGAMAVAEEIVEDP"
     gene            complement(2711..3382)
                     /locus_tag="VEL74_13700"
     CDS             complement(2711..3382)
                     /locus_tag="VEL74_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63630.1"
                     /translation="MPHAVVAPQRSRKTMTWRRRSACGLAAVLLLTAGGRADGAGMIS
                     FERAAVNVTGMKPQGRVFLFGVVWTDIPRSASRHTTFLSDGDGDGQARLEVGNEMPPI
                     AVWFAVDFEDGGVTIASRGTDVREMPFPWNAIAHGRDALTFPSERVYLAVVRPKTGAW
                     IVRAHDGKSLDADGNNDGSVRADLASLSGFGGSPAPPGKLEPGDVILAIDAERLMFTT
                     HQVGE"
     gene            3427..4587
                     /locus_tag="VEL74_13705"
     CDS             3427..4587
                     /locus_tag="VEL74_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYG63631.1"
                     /translation="MIETVAVANYRSLRELIVPLGRLNLITGPNASGKSNLYRALRLL
                     AETAQGGVIAALAREGGLGSTLWAGPEAISRAMRSHEMPVQGGPRKERVSLRLGFAGA
                     GLSYAIDLGLPIPSSSAFANDPEIKRECIWAGPHLRPASLLVDRRGPLVRIREGRSWQ
                     VLTQSLPAFESMLTQVADPARAPEVLALREEIRSWRFYDQFRADAGAPARESRLGTRT
                     PVLSHDGRDLAAALQTIREIGNGPALAEAIDDAFPGARLSIRNDNGRFEVELQQDGLL
                     RPLSAAELSDGTLRGLLWIAALLTPRPPALMVLNEPETSLHPDLMPALARLIRHAARE
                     TQVWVVSHASRLIAALEEDPDCQSITLEKDHGETRIVGQRLLDQPPWYWPER"
     gene            complement(5444..6073)
                     /locus_tag="VEL74_13710"
     CDS             complement(5444..6073)
                     /locus_tag="VEL74_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine cyclotransferase"
                     /protein_id="HYG63632.1"
                     /translation="MNRSNAEIVREYGPFPGADRVHGVTYDGQNVWFAAGDRLNAFDP
                     ASGETLRSLEVAAHAGTAFDGQHLFQLAEDRIQKIDPETGRVLATIPAPGGGGDSGLT
                     WAEGALWVGQYRDRKIYQVDPRTGAVLRTIESNRFVTGVTWVDGELWHGTWEGDESEL
                     RRLDPRTGEVLERLEMPPGAGVSGLESDGGDLFFCGGGSTGKVRAVRRP"
     gene            complement(6083..7303)
                     /locus_tag="VEL74_13715"
     CDS             complement(6083..7303)
                     /locus_tag="VEL74_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYG63633.1"
                     /translation="MDSLITAAARALAAGDPLGALKRVALRDDAPALALRGIAMAQLG
                     DLVRAKALLRRAARAFGPKEAVARARCVVAEAEIALVSRDLGWPAKALDAARATLEAH
                     ADRVNAAHARHLEVRRLLLIGRIDEAERRLAELDPAPLPPASRAVHELGVAGIAIRRL
                     RTEAARAALARARRAAHHARIPALTAEVESADLVLSTPAARLIAGGEERLLLLAEVEA
                     LLASDAWIVDACRYVVRHAGNVVSLARRPVLFALARALGEAWPGDVSRDTLVARAFGA
                     KLADESHRARLRVEIGRLRAELRTLAGVSATKRGFVLAPRRAGEVAVLAPPVEDEDAR
                     VLAFLADGEAWSSSALALALATSQRTVQRALDALEAAGKVQSFGRGRARRWMTPPVPG
                     FTTILLLPPPLPID"
     gene            7403..8158
                     /locus_tag="VEL74_13720"
     CDS             7403..8158
                     /locus_tag="VEL74_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018117176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HYG63634.1"
                     /translation="MTEHRTGTRQEWIAARLELIAAEKELTQRSDELARRRQELPWVR
                     IDKEYRFDTEEGSASLPDLFRGRSQLLVYHFMFGPDYSAGCPSCSAIADGFNGSVVHL
                     ANHDVTLCAVSRAPLAKLQAYKQRMGWTFPWASSFGSDFNSDFSVEFTEEQQREGGIE
                     YNYQREEPWNGAGAGAESFAAMSGTDAATYSRDRPGMSAFVLEDGVVYHTYSTYSRGL
                     DGLWGMYQWLDRAPRGRNEKGVWWRRHDEYGNR"
     gene            8276..8611
                     /locus_tag="VEL74_13725"
     CDS             8276..8611
                     /locus_tag="VEL74_13725"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HYG63635.1"
                     /translation="MKRNPWLPVLALSCLCLAAAVPPAAADDPPLANFYGNCVSPRLC
                     AVDAEASSDDGYITNYHWNWGDGTSTNGTKSDPSHTYAVAGTYTITLTITDNANQTDV
                     YSLPFQVPN"
     gene            complement(8681..10486)
                     /locus_tag="VEL74_13730"
     CDS             complement(8681..10486)
                     /locus_tag="VEL74_13730"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYG63636.1"
                     /translation="MRVKIRNVSVVLLWFLLLLPGANSVLAAPANERPRQLSQRGLEN
                     LTAFARLLAYVRFFHPSDQAAAADWDQVAIAGVQEAEKAVNADDLARTLEDFFAPLAP
                     TLRVYPDGGRPGVPGELSPPSGNPQVVYWVHNGVGLPATPPLTSYTSVREASPNAPPA
                     ETGLPRPEQPLEVSLGGGVSALLPLALYRDSQGATLPAVTLPMPRPDKPAGFRPSGKD
                     RATRLAGVALAWGVLQHFYPYFDVISVDWPAELRRTLTAAAKDRDDKAFVDTLRRMMV
                     PLRDGHAFIDHPTVVRTHQLPLAWDVVEGKWVITWTDPRLAPGLARGDVILSFNGKPA
                     GQVLANAMSLNPGATPQFIRVRALTMLTAGAKNEAVRLRVQRARGGTANVTVRRSVPF
                     GSSLNLLREPRPDKIAEVEPGIFYLDLNRLNDGDLIAALPALTAARGYVFDLRGYPYD
                     FGTTLLSHLATEPIRTTPVDIPIVTLPDRPWFRAERKQFTQPALTPRLTAPAAWITYG
                     EAVSYAETYLGIVEGHGLGEIVGEATAGTNGTVNYIALPGGYSVRFTGMRAIRHDGSQ
                     HFGVGIPPTIPVTRTLEGVRQGKDEYLERAIEAVR"
     gene            complement(10693..12339)
                     /locus_tag="VEL74_13735"
     CDS             complement(10693..12339)
                     /locus_tag="VEL74_13735"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HYG63637.1"
                     /translation="MKKLLIVTLVLIATAFLPLSASAQLTAKPLPSDAPYGYYEYLPL
                     GYNESAPQKWPLVLFLHGAGERGINGNPLSDVLSTGLPRNINTGTHYPFVMIAPQHTD
                     NNYNWSTTRLDELIEYAKIEYDIDPDRIYVTGLSMGGRGTWFYSKAHPEKLAAVLPIC
                     GADSGYDGSKLINVPAWIFHNWGDTEVTAAWSVGWANAIASAISGQSSNVMANYPGTV
                     TGPPGPGNNTDKTRTASFNAVTKAWTWNDNVVQVAGSHPTLTLYPGTSHGGWSNTYNN
                     STVWKWLLAQTRYNGTNKAPAVTITAPANNATTSSSVTIAAQVADSDGTLDPVAFYAG
                     STLLGTDSAPPYQFVWNNAPAGAHWITVKATDNDSSTSAALVKINVPVSTGQIITPAA
                     AGKADGSLYFPMGNAFDGQPTGLDASGAPTGGVNGYDAPSYSSRVGYIDLGPNWQKVR
                     IESTWTKYRVSSVGNQTPYVELWWDDDIDTVNDSGLTETRVNFNTAQSLSTGSTEPWL
                     RDRDLSASPVTPRARYLLCLSPSVMSNRAKEYAIVGSIVP"
     gene            complement(12492..>15731)
                     /locus_tag="VEL74_13740"
     CDS             complement(12492..>15731)
                     /locus_tag="VEL74_13740"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HYG63638.1"
                     /translation="SGPSRPWQLLLLSARTPTALDRATANLAGHLEAHPDLSLADAAF
                     TLQVGRRALEHRRAVVVQDVADARAVLEGGDPRRVLSAWCEDRERGVVFLFSGMGGQY
                     PDMGRGLYDSEPTFKTDVDRCAEILQPLLGLDLREVMYPAASSTKEVGEGTAGSVDLR
                     KMLRRSGSGDDEAAARLNRTRFAQPALFVLEYALARLWMEWGIRPQAMMGYSLGEYVA
                     ACLAGVFSLEDALALVAERARLIEELPEGAMLAVSLPEAEAAALLSDELSLAAVNGPE
                     QSVLAGPLPAVEELERDLASRGIACRRLQTTHAFHSKMMEPIRQAWAERVGRATLRPP
                     QIPYISNVTGGWITADQATDPAFWAGHTCQAVRFSEGVAELRRDPDRVLLEIGPGATL
                     ASLVLQHPASKGKEGEEALVVAALRHSYETQPDVAYTLTALAKLWLAGVRVDWSGVYA
                     RERRHRLVLPTYPFERQRYWIDTIDATRGERRAASRALAREGAHLYLPSWRRAPLSGA
                     GIPEPGRWLVFADGAGIGEAMAERLRGMGSDVALVHAGREEEPFTDLGGGDYRFDPRS
                     AEAHATLFNELGRVPDRIVHLTSISSPSPTLGFLGFQSLAALAGALVGRGVTEPVRLW
                     VIGDELHEVSGEEEPSPERTALLGAVKAIPGELPQVACRAVDVALPATAPTRERLLRQ
                     LLAELAADSPDRLVALRGNHRWAPAVEPLAPAPTAPAEGGLWLVSAGLGQAGFAFTRE
                     LAGANAKLLLLEPAGFPAREEWSDWLEGDADGVVGQLLRNARTLEEQGAELEVAGVDL
                     ADGALLREVVEAAEARWGTVRGTVWLAEPGTSEGHLAGALTGLRSLDALLATRGLTSA
                     LLVVPEGTPGAEAAALGFLADTFATGSALAGGTPWTSVAWALPPEEGATAAARRLLGG
                     AGALQVLASPRPLPDGWSRLDTFPESEATARTGVGSYPRPSLRVEYVAPRNETEEYIA
                     RIWKDLLGVAQVGIHDNFLDLGGDSLLATRLVARMRDTFHLELPVRLFFEKSTVAELA
                     EAVAALRQQEQERETEELLAKVQSLSEEELEQELLRMEGMVGEEEPARG"
BASE COUNT         2451 a         5545 c         5357 g         2378 t
ORIGIN      
        1 ccctactcag ggtcgccggc gttcacccgc cggctggtct gcggctcagg tcggccctct
       61 gctacgcgaa ggccggccgc acgaacggcg ctgccggagg gaagctcccc tccgggaacg
      121 ctaccatccg gtcgccggcc ctcagcttct cggcggcgtc tcgaaacgcc gccacgaacc
      181 ggctgtacgc ctcccgcaga ctccggtagg ccttcttgct cgctgtgtgg aacagcggcg
      241 cgggtgattt cttcgtccga ttcggacggg tgtgtggctg ctgcctccgc accgcgtcca
      301 ctcccagagg ctcgataccc ttcgcctccc tcgccacccg ggcctcttcc tcgatctcgg
      361 ccaccagctc ggcgatccgc cgccggtact cctccggcgg caggtgcttc cagcacggca
      421 gcggatcgag ggtcagcgtc tcgacggtcg cgaactggcc cggctcaggt tccttacctc
      481 gcagccggta ccggtactcc tgggtgcggt cgtgccagac cccctccatg ggattgccgg
      541 tcaacagggc gttgaccgcg tgagcaccag gccattcccg ggggctgccc accaatccct
      601 ccttggcccc gtgcgaaagg gcgtacttta gacgagcgat ctgcgccccc tcctcgtcgc
      661 tgaccacgat gtgctggtag cgccggcccc agaacttctc cttccagtcg gccaggcggc
      721 cgagctcccg cgccaggttg gagttcaggt agcccatgaa cgcggccatc tcggctgcgt
      781 cctccacttc catcaaaagg tgataatggt tggaggcaaa gacaaagcaa atcaacttca
      841 cagggtacaa gcgcttcgcc cgagccaaga caccgaggac ggtctcccgg acgtaggtac
      901 gaggggcgag cagcaagcgg ctttgaacgg tgcggcaggt cacctcgacg agggacccgc
      961 cttctggcac atgacggagt tttctcggca tgccaggaaa agacggaaaa cgccgaaaaa
     1021 atcgctcaat tttagtgtat ctgcgagcgg ctatacctaa attagacgga ggaagtgcca
     1081 gccaagcagc tcgagtgcac gaggtgactc agacgttccc acccgcggcc gccggatgag
     1141 gcgcctcgta ctggtagtgg gctccggggc tccaaggcgt gctaaagtcg cgggatcacg
     1201 aggaccacac atggtacgtc tgtctcacgg cgccatcgcc atccagaccg tgatcctgtc
     1261 cgtattcctg accgtatcgc ctgccaccgc gcaggagccg gaggggccct ccggcgggct
     1321 cgcggcgttc gcgggcgagg aggaggttcg ggacgccatc gctcccgcac agctcgcgga
     1381 gattcgggcg tcggtcgccg cccatgagcg acggaaaggg ctgaccgcgg cgccgggcca
     1441 ggaagaggca ccgttcctct ttcccttttt cccgcacgcc ggcgttcagg gtcgagacct
     1501 ctatctgtcc aatttcaccg atcaggacct gagctccgtc accgtccgcg actgggactg
     1561 ctccgcgcag gcctacgacg ggcatcatgg ccacgactcg ctcatccgca gcttccgcga
     1621 gcaggcgatc ggcgtgccca tcttcgccgt gcgcgacggc gtggtggcgg cgactcacga
     1681 cggcgagccg gacatgaaca cggtgtggga ccccacgacc aaggccaact acgtgctcgt
     1741 cgatcatgga aacggctatc tcacctggta cctccacatg cggaacggca gcgtggcggt
     1801 ctcccccggg cagaccgtga ccgccgggac acagctcggc ctgaccggca gcagcgggct
     1861 cagcaactgg ccgcatctcc acttcgagac ccacaaggac ggcacctgga ccgagccctc
     1921 ggccgggccg tgccgcccgg gggacagcct ctgggtctcc cagccgcccg tgccgcggga
     1981 tttctacgtc gcggatttct acctggccca aggcgccatc ctgatcccgg acttcaccac
     2041 ctggctccac gacccggcaa cgcggaaggc cactttcctc aaaggggtcc agagggtggg
     2101 agcgcgcgtg gacatgcgga gcctccccgc gcattccacc tatcgcatcc agatgctgga
     2161 cccgcgcggg aaagtggtct ttcaagactc gggctctttc gaaaaccccg tcccgcagaa
     2221 gctcgccgtc gcgaccttct ggttcgacat gaacctctct cccggcacct ggaggttccg
     2281 ggccgacttc aacgacagcc cggctgtcga cgctcccttc cgggtggtcg ccacctcgcg
     2341 gcaggtccgg aaccgccgtc cgaacgccgt caagacgctc ctcactccgg cgcgcccggt
     2401 ggccggccag gcgatgacct gtgaggtccg ggctccgctg atcggcaggg acccggacta
     2461 cgacatcgtg agctatcgct acgagtggaa ggtcaacaat cgggtggtgc gctccgtcac
     2521 cagcgcggcc ctgacggatc tcctggccgc cggcaaggcg aagcccgggg atcgggtgag
     2581 ctgccgtgtc accccgtcgg acggcaaggc gctgggagca atggcggtgg cggaggagat
     2641 cgtcgaggac ccgtaggctc gcggggaaag gccacccgcg gcaggaacag tccgccctcc
     2701 ggggaagccc ctactccccc acctggtgcg tggtgaacat cagccgctcg gcgtcgatgg
     2761 caaggatgac gtcgcccggc tccagcttcc cgggtggtgc cggggagccg ccgaagcccg
     2821 acaggctggc caggtcggcc cggaccgagc cgtcgttgtt gccgtccgcg tcgaggctct
     2881 tgccgtcgtg ggcgcggacg atccaggctc ccgtcttcgg ccgcacgacg gcgagataga
     2941 ccctctcgga agggaacgtg agcgcatcgc ggccgtgggc gatggcgttc caggggaagg
     3001 gcatctcccg cacgtcggtg ccgcgcgagg cgatggtcac acccccatcc tcgaaatcca
     3061 cggcgaacca gaccgcgatg ggaggcatct cgttgccgac ctccaagcgg gcctggccgt
     3121 ccccgtcccc gtcgctcagg aaggtggtgt gacgcgaggc gctccggggg atgtcggtcc
     3181 acacgacacc gaacaggaac acccggccct gcggcttcat ccccgtgacg ttgaccgccg
     3241 cccgctcgaa cgagatcatc ccggccccgt ccgcgcgccc gccggccgtc agcagcagaa
     3301 cggccgccag accgcaggcc gatcgtctcc tccacgtcat ggtcttccgg ctgcgctggg
     3361 gagcgacgac ggcatgaggc atgggggttc ctcctggcag aattctacca gcgctaagct
     3421 tgggtcatga tcgagacggt cgccgtcgcg aattaccggt ctcttcgtga gctgatcgtt
     3481 ccgctcggcc ggctcaacct catcacgggc ccgaacgcca gcggcaagtc gaacctctat
     3541 cgcgccctgc ggctgctggc cgagacggcc cagggaggcg tcatcgccgc cctggcgcgg
     3601 gagggcggcc tcggctccac tctgtgggcg gggcccgaag cgatctcccg ggccatgcgg
     3661 tcccacgaga tgcccgtcca gggcgggccg cgcaaggagc gcgtgagcct ccgcctcggg
     3721 tttgccggcg ccgggctgag ctacgcgatc gacctcggcc tcccaatccc cagcagctcg
     3781 gcgttcgcca acgaccccga gatcaaacgg gagtgcatct gggcaggccc ccacctccgg
     3841 ccggcgtcgc tcctggtcga ccgccgcgga ccgctggtcc ggatccggga gggccggagc
     3901 tggcaggtgc tgacccagag cctccccgcg ttcgaaagca tgctgaccca ggtcgcggac
     3961 cccgcgcggg cgcccgaggt gctggccttg cgggaggaga tccggtcctg gcgcttctac
     4021 gatcagttca gggccgacgc cggcgcgccc gcccgggagt cccggctcgg cacgcgcacg
     4081 ccggtcctgt ctcacgacgg ccgcgatctg gccgcggcgc tccagaccat ccgcgagatc
     4141 ggcaacggtc ccgcgctcgc cgaggccatc gacgacgcgt tcccaggggc ccggctgtcg
     4201 atccgcaacg acaacgggcg cttcgaagtg gagctccagc aggacgggct cctgcggccg
     4261 ctcagcgcgg cggagctgtc ggacggcacg ctgcgcggcc tcctgtggat cgccgcgctg
     4321 ctgacgcccc gccccccggc cctcatggtc ctcaacgagc cggagaccag cctccacccg
     4381 gacctgatgc ccgccctggc gcgcctcatc cggcacgccg cccgcgagac ccaggtatgg
     4441 gtggtctccc acgcctcgcg cctcatcgcg gccctggaag aagacccgga ctgccaatcc
     4501 atcaccctcg agaaggatca cggcgagacc cggatcgtcg ggcaacgcct cctggatcag
     4561 cccccctggt attggccgga gcggtaggca tccagacatg gaacgtccag ccagcagagg
     4621 cggagggagc ggacctccgc ctcctggctg acctcctgtc ggacggagga atcctcagga
     4681 gcgcggcctt ggacacggcg gcgccccgtc acgattcagt tgtcaatgaa ccgggagcgc
     4741 cgttccaggc ccggtgaggc ccgaaacgag gctctccttc ttcagccggc tcgcgggagg
     4801 cgttccgggc cgattcctca gtactacagc gccactcctc aacacgtcgc cgcgactcct
     4861 caacactacg gagtcactct ggagcgctcc gggatggctc ctcagtgcta cggagccgtc
     4921 cctcaatgct tcaccgccgc tcctcagtgc tacggggcca cttcggaatg ttccgagccg
     4981 gcccgtcagc actgagggat gactccggag cactccggga cggcccgtca acactccggc
     5041 gccgctcctc agtactccgc cggcgctcct caacactgcg gagtcatcct ggagtgttga
     5101 gggccggccc ctcagcgcta cgggaccgtc cctcagtcct tcgctgccgc tcctcagtgc
     5161 tacggaatca ctttggagtg ttccggaacg gcggcgaagc attgagggat gacggcagtc
     5221 gtagccgcgg cgagtcagaa tctacaaagg agatcgcccc gatgtcacca gagtcgacat
     5281 cgccgcctct tgtggtaggc tcttggccgt gcagtggcag ttctttgaca atcgatgtta
     5341 actgttcaac cgcggcgccg ggtggggggc gccgcgcttg gacgagccac tcaggtgggc
     5401 tatccagccg ccgcggcctc acgctgcccg tggcggagcc tcgtcagggc cggcggacgg
     5461 ccctcacctt gccggtgctt cccccgccgc agaagaacag atcgccgcca tcggattcga
     5521 gccccgaaac gcccgctccg ggcggcatct ccagtctctc cagaacctct cccgttcgcg
     5581 gatcgagccg ccgcagctcg ctctcgtcac cttcccaggt gccgtgccag agctctccgt
     5641 cgacccaggt gaccccggtg acgaagcggt tggactcgat cgtgcgcagg accgcccctg
     5701 tccggggatc gacctgataa atcttccggt cccggtactg ccccacccag agcgcccctt
     5761 cggcccacgt cagccccgag tcaccgccgc cgccgggcgc cgggatcgtg gcgagcaccc
     5821 ggccggtctc ggggtcgatc ttctggatgc ggtcctcggc gagctgaaac aggtgctggc
     5881 cgtcgaaggc cgttcctgca tgcgcggcga cttcgagcga gcgcagcgtc tccccgctgg
     5941 caggatcgaa agcgttcagt ctgtctccag cggcaaacca gacgttctgg ccgtcatagg
     6001 tgactccatg caccctgtcg gcacccggaa agggcccata ctcacggacg atctcggcgt
     6061 ttgatcggtt catgggtcca tcctagtcaa tcggcagagg cggagggagt aacaaaatgg
     6121 tcgtgaatcc cggcacgggc ggcgtcatcc agcgacgggc tctcccgcgg ccgaacgact
     6181 gcaccttgcc cgcggcctcg agcgcgtcga gcgcccgctg cacggtgcgc tggctcgttg
     6241 ccagcgccag cgccagggcc gagctcgacc acgcttcccc gtcggcgagg aaggcgagca
     6301 cccgcgcatc ctcgtcttcg accggcggcg ccagcacggc cacctcgccg gcgcggcgcg
     6361 gcgccagcac gaaaccgcgc ttcgtcgcgc tcacgccggc cagcgtcctg agctccgcgc
     6421 gcagccgccc gatttcgacg cgcaaccgcg cgcgatgcga ttcatcggcg agcttcgctc
     6481 cgaaggcccg cgcgacgagc gtgtcccgtg atacgtctcc cggccacgct tcgcccagcg
     6541 cgcgggcgag cgcgaacagc accggccgcc tcgcgagcga gaccacgttg cctgcatggc
     6601 gcacaacata gcggcacgcg tccacgatcc atgcgtccga cgccagcaac gcttcgacct
     6661 ccgcgagcag gaggagccgc tcctcaccac ccgcgatcag gcgcgcggcg ggcgtgctca
     6721 gaacgaggtc cgcgctttcc acctccgctg tcagcgcggg gatgcgcgcg tggtgcgccg
     6781 cgcgcctggc ccgagcgagc gcggcgcgtg ccgcctccgt cctgaggcgc cggatcgcga
     6841 tccccgcgac ccccagctcg tggacggccc gcgacgcggg agggaggggc gcggggtcga
     6901 gctcggcgag cctgcgctcg gcttcgtcga tgcgcccgat caggaggagg cgccggacct
     6961 cgagatgccg cgcgtgcgcg gcgttgaccc ggtcggcatg ggcttcgagc gtcgcccgcg
     7021 ccgcgtcgag cgccttcgcg ggccagccga ggtcgcgcga gacgagcgcg atctcggcct
     7081 cggcgacgac gcacctcgcg cgggccacgg cctctttcgg accgaaggcc cgcgcggcac
     7141 gccgcaggag agccttcgcg cgaacgagat caccgagctg cgccatcgcg atgccgcgaa
     7201 gcgcgagagc cggcgcgtcg tcgcgcaggg cgacccgctt caatgcgccg aggggatccc
     7261 ccgcggccag cgcgcgcgcc gcggcagtga tcagcgagtc catcggaatc ccgtcacact
     7321 tgcaactccc accgctcgat atcccggggc tagactctat ctcatgaccg gtaacgaacg
     7381 agcgcagaag gagagaaaag cgatgacgga gcacagaacc gggacacgtc aagaatggat
     7441 tgcagcccgc ctcgagctga tcgcggcgga gaaggagctc acccagcgca gcgacgagtt
     7501 ggcgcggcgg cggcaggagc tgccatgggt ccggatcgac aaggagtacc ggttcgatac
     7561 cgaggagggg agcgcctcgc tgccagacct cttccgcggg cgctcgcagc tcctcgtcta
     7621 ccatttcatg ttcgggcccg actacagcgc cggctgcccg tcctgctcgg cgatcgcgga
     7681 cgggttcaac gggtccgtcg tgcatctggc caaccacgac gtcacgctct gcgcggtgtc
     7741 gcgggcgccc ctcgcgaagc tgcaagccta caagcagcgg atgggatgga cgttcccctg
     7801 ggcgtcctcg ttcggcagcg acttcaattc cgacttcagc gtcgagttca ccgaggagca
     7861 acagcgcgag ggcggcatcg aatacaacta tcagcgcgag gagccgtgga acggcgccgg
     7921 cgcgggggct gaatccttcg cggcgatgag cggaaccgac gcggccacct actcgcgcga
     7981 ccggcccggc atgagcgcgt tcgtgctcga ggacggcgtc gtctaccaca cgtattccac
     8041 ctattcgcgc gggctggacg gcctctgggg catgtaccag tggctcgacc gcgcccccag
     8101 gggccgcaac gagaagggcg tctggtggcg ccgccatgac gagtacggga atcggtgagc
     8161 gaggtccgca tgtcgagccg ccgggctcgg atcacgcggt gggagggcgg cagcccggtc
     8221 aagctccctt tgcgtttcgg cgtatactgc tacttattaa ttaggaggta gaccgatgaa
     8281 acggaacccg tggcttcccg tcctcgccct ctcctgtctc tgcctggcgg ccgccgtccc
     8341 acccgcagcc gccgacgatc cgccgctcgc caacttctac ggaaactgcg tgtccccccg
     8401 cctctgcgcg gtggacgcgg aggcctcgtc cgacgacggc tacatcacga actaccactg
     8461 gaactggggg gacggcacgt cgacgaacgg caccaagtcg gacccgagcc acacctacgc
     8521 ggtcgcgggc acctacacga tcacgctcac gatcaccgac aacgccaacc agacagacgt
     8581 ctactcgctg ccgttccagg tgccgaactg agcttcgagc cgacagcgag gcgttgaacc
     8641 cgccggccga gcgctccggc cggcgggtgc ctctcgattc ctaccgcacc gcctcgatcg
     8701 ccctctccag gtactcgtcc ttcccttgcc gcacgccttc cagggtgcgg gtgacgggga
     8761 tggtcggcgg gatgcccacg ccgaagtgct gggagccgtc gtggcgtatc gcccgcatgc
     8821 cggtgaagcg gaccgagtag ccgccgggga gcgcgatgta gttgacggtg ccgttggtcc
     8881 ccgccgtcgc ctcgcccacg atctcgccca gcccgtgccc ctcgacgatc cccaggtagg
     8941 tctccgcgta gctgaccgcc tcgccatagg tgatccaggc ggccggcgcc gtcagccggg
     9001 gggtcaaggc cggttgggtg aactgcttcc gctccgcgcg gaaccagggg cggtccggga
     9061 gagtgacgat cgggatgtcc accggcgtcg tgcggatcgg ctccgtcgcg aggtgcgaga
     9121 ggagcgtcgt gccgaagtca tagggatatc cccggaggtc gaagacgtaa ccccgcgccg
     9181 cggtaagcgc cggcagggcg gcgatcaggt ccccgtcgtt gagccggttg aggtcgagat
     9241 agaagatgcc cggctcgacc tcggcgatct tgtccggccg cggctcgcgg aggaggttaa
     9301 ggctgctgcc gaaggggacc gagcgccgga cggtgacgtt ggcggtgccg ccgcgggccc
     9361 gctgcacacg cagccggacc gcctcgttct tcgcccccgc cgtcagcatc gtcagggccc
     9421 gcacgcggat gaactgcggg gtggcccccg ggttcaggga catcgcgttc gccagcacct
     9481 ggccggccgg cttgccgttg aagctcagga tgacgtcgcc gcgtgcgagg cccggcgcca
     9541 gccgcggatc ggtccaggtg atcacccact tcccttccac gacgtcccag gccaggggga
     9601 gctgatgcgt ccgcaccacc gtcgggtggt cgatgaaggc gtggccgtcc cggagcggca
     9661 ccatcatccg gcgcagggtg tctacgaaag ccttgtcgtc ccggtccttc gccgcggcgg
     9721 tcagggtgcg ccgcagctcg gccggccagt ccacggagat gacgtcgaag tagggataga
     9781 agtgctggag caccccccag gccagcgcca cgccagccag ccgggtcgcc cggtccttgc
     9841 cggaaggtcg gaagccggcg ggcttgtcgg gccggggcat cggcagcgtc accgccggca
     9901 gggtggcgcc ctggctgtca cggtagagcg ccagcggcag cagcgccgag actccgcctc
     9961 cgagcgacac ctccagcggc tgctcgggcc gcggcagccc cgtctcggcc ggcggcgcgt
    10021 tgggcgaggc ctcccgcacg ctggtgtagg aagtgagggg aggggtcgcc ggcagcccga
    10081 ccccgttgtg gacccagtac accacctgcg ggttgcccga aggggggctc agctctccgg
    10141 gcacgccggg gcgcccgccg tcggggtaga cccgcaatgt cggggcgaga ggggcgaaga
    10201 agtcctccag ggtgcgcgcg aggtcgtccg cattgaccgc cttctcggcc tcctggactc
    10261 cggcgatggc cacctggtcc caatccgccg ccgcggcctg gtcgctggga tggaagaagc
    10321 ggacgtacgc caggagccgc gcgaaggccg tcaggttctc gaggccgcgc tggctgagct
    10381 gccgcggccg ctcgttggcc ggcgctgcga ggacggagtt ggccccgggg aggaggagca
    10441 ggaaccagag cagcacgacg gagacgttgc ggatcttcac tcgcattcca gaacctccaa
    10501 gtcgttcaac cgtaccctgc ccgacccgga tggctctcca ccgcggagcg ccacccgcgt
    10561 ccccagccgc ccaaccctcc ggccggacta cttccatagt gggttcagtc tctcaaacca
    10621 ggactggaat gtcaggaggt ggcggaggcg gacccgcgaa acgccggcgc gcctccgccc
    10681 gtacgagcct tgctagggca cgatcgatcc cacgatcgcg tactccttcg cgcggttgct
    10741 catcacggag ggcgagaggc acagcagata gcgggccctc ggcgtgaccg ggctggcgct
    10801 caggtcacgg tcccgcagcc acggctccgt cgagccggtg ctcaggctct gcgcggtgtt
    10861 gaagttgacg cgggtctcgg tgaggccgct gtcgttgacg gtgtcgatgt cgtcatccca
    10921 ccacagctcg acatacgggg tctgattgcc gacgctcgag acacggtact tcgtccaggt
    10981 ggactcgatt ctcaccttct gccagttggg ccccaagtcg atgtagccga cgcggctgga
    11041 gtaagaggga gcatcgtagc cgttgacccc gcccgttggg gctccgctcg cgtccaggcc
    11101 ggtcggctgg ccatcgaaag cgtttcccat cgggaagtag aggctgccat ccgccttgcc
    11161 cgcggcggcc ggcgtgatga tctgaccggt cgacaccggc acattgatct tcaccagggc
    11221 ggcgctggtg gagctgtcgt tgtcggtcgc cttgacggtg atccaatgag ctcccgccgg
    11281 cgcgttgttc cagacgaact ggtagggcgg ggcgctatcg gtgccgagga gtgtggagcc
    11341 cgcgtagaac gcgaccgggt cgagcgtgcc gtcgctgtcg gcgacctgcg ccgcgatggt
    11401 gacgctcgac gaagtcgtgg cgttgttcgc gggcgcggtg atggtcaccg cgggcgcctt
    11461 gttggtgccg ttgtagcggg tctgtgccaa cagccatttc cagactgtcg agttgttata
    11521 ggtgttgctc caaccgccgt gtgaggtccc cgggtacagg gtcagagtcg gatggctgcc
    11581 agccacctgc accacgttgt cgttccaggt ccaggccttg gtcacggcat tgaagctcgc
    11641 ggtcctggtc ttgtccgtgt tgttccccgg tcccggaggg ccggtgacag tgccgggata
    11701 gttggccatc acattggagc tctggccgct gatcgcgctg gcaatcgcgt tcgcccagcc
    11761 gacggaccag gccgcggtaa cctcggtgtc gccccaatta tgaaagatcc acgccggaac
    11821 gttgatcagc ttcgatccat cgtaaccgct gtccgcaccg cagatcggca gcaccgcggc
    11881 cagcttttcg ggatgagcct tggagtagaa ccaggtgccg cgtccgccca tgctcaagcc
    11941 ggtcacgtag atgcggtccg ggtcgatgtc gtactcgatc ttggcgtact cgatgagctc
    12001 gtcaagacga gtggtgctcc agttgtagtt gttgtcagtg tgttggggcg cgatcatcac
    12061 gaagggatag tgagtacccg tgttgatgtt cctcggcaac cctgtcgaga gtacatcgct
    12121 caagggattt ccattgatgc cacgctcgcc ggcgccatgc aggaacaaca ccaggggcca
    12181 tttctggggt gcggattcgt tgtagccgag cggcaggtat tcgtagtagc cgtagggggc
    12241 gtcggagggc aacggcttcg ccgtgagctg tgccgaagca gagagtggta agaatgccgt
    12301 ggcgatgagg acaagagtga caatgagcag ttttttcatg gcacgccttt ccgtgaagct
    12361 ggttctcggg agttgatcgg cggcacaggc ctccaccaac acttctgcgt attggcagat
    12421 gcaaatatta agccggactg aacaggccgg agagcctggc gggagagcgg gagcgagagg
    12481 gacgggatct tctaccccct cgccggctcc tcctccccca ccatgccttc catgcggagc
    12541 agttcctgct ccagctcctc ctcggagagg ctctgaacct tggcgagcag ctcctcggtc
    12601 tcccgctcct gctcctgctg gcgcagggcg gcgacggctt cggccagctc ggcgacggtg
    12661 gacttctcga agaagagccg caccggcagc tcgagatgga aggtgtcccg catgcgggcg
    12721 acgagacggg tggcgagtag ggagtccccg ccgaggtcca ggaagttgtc gtggatgccc
    12781 acctgggcga cccccaggag gtccttccag atacgggcga tgtactcctc ggtctcattg
    12841 cgcggggcga cgtactccac ccgcaggctc ggccgcgggt aagagcccac gcccgtccgc
    12901 gccgtggcct cggactcggg gaaggtgtcg agacggctcc agccatccgg cagcggacgc
    12961 ggcgaggcca gcacctggag cgctccggcg cctccgagga gacggcgcgc cgccgcggtg
    13021 gctccctctt caggagggag cgcccaggca acgctggtcc agggggtgcc cccggcgagg
    13081 gcgctgccgg tggcgaaggt gtccgccagg aagcccagcg cggcagcctc agccccgggc
    13141 gtcccctcgg gcaccaccag gagcgccgag gtcaggccgc gggtggcgag gagggcgtcc
    13201 agcgaccgca gaccggtgag cgcgcctgcc agatgcccct cgctcgttcc gggctcggcc
    13261 agccagacgg tgccacggac cgtcccccag cgcgcttcgg cggcctccac cacctcgcgc
    13321 agcagggcgc cgtccgcgag gtccaccccc gccacctcca gctcggcccc ctgctcctcc
    13381 agggtccggg cgttgcggag gagttgtccg acgaccccgt cggcgtcccc ctccagccag
    13441 tcgctccact cctcgcgcgc cggaaagccg gcgggctcga ggagcaggag cttggcgttc
    13501 gcgccagcca gctcgcgggt gaaggcgaag cccgcctgcc ccaggcccgc ggagaccagc
    13561 cagaggccgc cttcggccgg agcggttgga gctggcgcca gcggctccac cgccggagcc
    13621 cagcgatggt tgccgcgcag ggcgacgagg cggtccggcg agtccgccgc cagctcggcc
    13681 aggagctgcc ggaggaggcg ctcccgggtg ggagcggtgg ccgggagggc gacgtccacg
    13741 gcccggcagg ccacctgggg cagctctccc gggatcgcct tgaccgctcc gagcagcgcg
    13801 gtgcgctccg gcgacggctc ctcctcgccg ctcacctcgt gcagctcgtc gccgatgacc
    13861 cagagacgga caggctcggt gacgccgcgg cccaccaggg cgccggcgag ggcggccagg
    13921 ctctggaagc caagaaaacc caaggttggg gagggagaag agatggaggt caggtgaacg
    13981 atgcggtccg gtactctacc cagctcattg aacagagtgg catgggcttc cgcggagcgg
    14041 ggatcgaagc gatagtcccc tcctcccagg tcggtgaagg gctcctcctc gcggccggcg
    14101 tggaccaggg cgacatccga gcccatacca cgcaggcgct ccgccatggc ctcgccgatc
    14161 ccggcgccgt ccgcgaagac cagccagcgg ccgggctccg ggatgccggc tcccgaaagc
    14221 ggcgcccgcc gccaggacgg caggtagaga tgggccccct cccttgccag ggcgcgggac
    14281 gccgcccgcc gctcgccgcg cgtggcgtcg atcgtgtcga tccagtagcg ctggcgctcg
    14341 aagggatagg tggggagcac cagccggtgg cgccgctccc gcgcgtagac gccgctccag
    14401 tccacccgga ccccggcgag ccagagcttg gcgagagcgg tcagggtgta ggcgacatcc
    14461 ggctgcgtct cgtaggagtg gcgcagggcg gcgacgacca gggcctcctc accttccttc
    14521 cccttgctcg cggggtgctg cagcaccagg ctcgccaggg tggcgccggg gccgatctcc
    14581 agcagcacgc ggtccggatc gcggcgcagc tcggcgaccc cctcggagaa gcggaccgcc
    14641 tggcaggtgt ggcccgccca gaaagccggg tccgtcgcct ggtccgccgt gatccagccg
    14701 ccggtgacgt tcgagatgta ggggatctgc ggcggccgga gcgtcgcccg gcccacccgc
    14761 tccgcccagg cctggcggat gggctccatc atcttcgagt ggaaggcgtg cgtggtctgg
    14821 aggcggcggc aggcgatgcc ccgcgaggcc aggtcccgct ccagctcctc caccgccggg
    14881 agcggcccgg cgagcaccga ctgctcggga ccgttgaccg ccgccagcga cagctcgtcg
    14941 ctcagcaggg cggccgcctc cgcctcgggc aggctgacgg ccagcatcgc cccctcgggc
    15001 agctcctcga tgagacgggc gcgctcggcg accagcgcca gggcgtcctc cagcgagaag
    15061 acgccggcga ggcaggcagc tacgtattcg cccaggctgt agcccatcat cgcctgcggc
    15121 cggatgcccc actccatcca cagccgcgcc agcgcgtact cgaggacgaa cagcgccggc
    15181 tgggcgaagc gggtgcggtt caggcgcgcc gccgcctcgt cgtccccgga gccggagcgg
    15241 cggagcatct tgcggaggtc gacgctaccc gcagtcccct ccccgacctc cttagtagaa
    15301 gacgccgccg ggtacatcac ctcccgcagg tccagcccga ggaggggctg gaggatctcg
    15361 gcgcagcggt ccacgtccgt cttgaaggta ggctcggagt cgtagagccc gcgccccatg
    15421 tccgggtact ggccgcccat gccggagaac aggaagacca ccccgcgctc ccggtcctcg
    15481 caccaggcgg agagcacccg gcgcgggtcg cccccctcca ggaccgcccg agcgtcggcg
    15541 acatcctgga ccaccacggc ccggcggtgc tccagggccc gccgccccac ctggagggtg
    15601 aaggcggcat cggcgaggga gagatcggga tgggcttcga gatgcccggc gaggttggcg
    15661 gtcgcccggt ccagcgccgt aggggtacgg gccgagagca gcaggagctg ccaggggcgc
    15721 gacgggccgg a
//
LOCUS       DASPJI010000507         8283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_201676, whole genome shotgun sequence.
ACCESSION   DASPJI010000507 DASPJI010000000
VERSION     DASPJI010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8283)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201676"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2007
                     /locus_tag="VEL74_13745"
     CDS             <1..2007
                     /locus_tag="VEL74_13745"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HYG63639.1"
                     /translation="GGGCLHVVARERLADATAFAELMARQPVDVLKIVPSHLAALLTA
                     ESPERSLPRRLLVLGGEPLPWSLVERVRSLAPECRLFNHYGPTETTVGVLAGAVEIQR
                     HEMGEAGGTRGSGSTCAPLGWPLGQTRGWVVDRHLRLVPAGVPGELCVGGPQVTRGYL
                     GRPDLTAERFIPDPFGGGAGERLYRTGDLVRRRPSGPIEFLGRTDHQVKVRGFRIELG
                     EIEAALATLPGVREALVVAREDTPGERRLVAYVAGDVSETAVDTLRASLREQLPDYMV
                     PAAFVTLTTLPLTSNGKVDRKALPAPEPPRPAESHSAMQTPIEDALAGIWAEFLGLEQ
                     VGATDHFFDLGGHSLLAVRLMSRIEHVFGVKLPISTLFEAPTVERLAGVLLRGSVPAR
                     HSPLVRLHTGGTGRPLFLVHPIGGDVSSYVELARRLGADRPVYALQPVTVEPGAERAA
                     PSTMEEMARQYLAAMRQVQAGGPWLIAGWSFGAILAYEMAQQIESSGDTVALLAMIDP
                     SAPPEARNERVDDIQLLAGFAGLGRPSERQRELLREMLAEVDVEAGLDRLLEFGQAEG
                     VLPPDVGKPWLRERFDLFSRNVQVLRGYVARPYGGRVTFFRAGASLAQGTMELTSGWS
                     TLAHTEAHLVDSDHVSMLRRPALDHLVEQLSTDLAAVEAGAEAP"
     gene            2004..2750
                     /locus_tag="VEL74_13750"
     CDS             2004..2750
                     /locus_tag="VEL74_13750"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG63640.1"
                     /translation="MKELIQDLRFSFRLFRKSPGFTAAVVLTLGLGIGVNVAIFSVVD
                     SVLVRPLPFRDGHQLVHLRYGKPNLGPDTIRFSVPESHDYRRHSRTLAGLREYHTMSF
                     TLLGGEEPNQVLVGVVSANFCDVLGIKPMLGRAFVPEDEQPGAPSVLLLSNRYWRSRF
                     GGNPNIVGRALRMNGRSIQVVGVLPPLPDFPRADDVFVPTSACPFRSNPEVVEGRDTR
                     MINLFGRMRQGVTLEQARAEMSLLAKPPAE"
     gene            2815..3432
                     /locus_tag="VEL74_13755"
     CDS             2815..3432
                     /locus_tag="VEL74_13755"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYG63641.1"
                     /translation="MNGKFLPTLQILLVTVVLLLLITCANVGNLSLARLLSREREVVL
                     RAALGAGRLRLIRQLLTESTALALIGGAVGLLFAAVTMSSLTAFARRFTPLANRIDIN
                     GRVLLFTLGIAVLSGLLFGLFPTLHAYRRNLGALLRQGGGHSTHDRGRQRLRSTLVVL
                     QVAVSFVLLIAAGLTTRSAIRLQRVDGGFDPRNVVALSLELPSAR"
     gene            3657..4439
                     /locus_tag="VEL74_13760"
     CDS             3657..4439
                     /locus_tag="VEL74_13760"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYG63642.1"
                     /translation="MLAGRAFLASDDANGLPVTIINQSLARRFWPAESPLGKRIDIGE
                     TGAPIWLTVVGIVGDVHHAGLDDAAEDAFYVPFLQYGGVAMNLFVRTTGEPILLPPAL
                     RKAVHEIDPEQSISDISMMEQIRSDSIAPMRLTATLLSLLGLLAFAITVAGLNGVIAY
                     STTEQTQEIGIRAALGARRTVLLHLVMRQGMALVLLGLALGWAGALGGGKLLASRLFG
                     VEPSDPVTFLLASLALLVCGGAACFFPARRAAFTDPMVALRS"
     gene            complement(4457..5578)
                     /locus_tag="VEL74_13765"
     CDS             complement(4457..5578)
                     /locus_tag="VEL74_13765"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HYG63643.1"
                     /translation="MKVRVPPTLLFGRGRMADVPAVLGELGAERPLVVTDPGVVSAGL
                     VERLLGELAGAGSEAEVWDGVAQDPDEGHAARCAERIAAGGHRSVIAFGGGSVIDVAK
                     VAAVLATNGGKTADYFGFEKVRHPSLPLLAVPTTPGSGAEVSSHAVLVQLSPRRKEVI
                     AGLHLLPLATLVDPELIVTLPSSHLTWSALDGFIHAVEAFLARRATPFTDMFARQAAP
                     AIARALPRVLADRSDFAACEELSLGCLYSGLAMANANAGAIHALGYPLTGRYGIPHGL
                     ANALMAAPALERIGTGNPGRCAELARLLGDGEDTLAGRVRHLLETLGVDRGLAGVGVR
                     EEDLPALADEATRFRPVLENTPVALDRNDLLAVYRAAWA"
     gene            complement(5575..6603)
                     /locus_tag="VEL74_13770"
     CDS             complement(5575..6603)
                     /locus_tag="VEL74_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HYG63644.1"
                     /translation="MAHSSIATAAEEEREAGPVIGSPEWIAEYSLSTRTACRKAQLDI
                     ARADERVFSIEGDLGQYTVPFHAEFPRRFLQMGIAEADMVGTAAGLAMRGKIPFVNSF
                     AAFLAFRACEQVRLEVAYHRSNVKLVGSYAGISGGAAAGTHHSYEDMAVVRAMPNVVL
                     LSPADSVETWKATWGAYAHEGPVYLRVGRAETPQVYFGDYEFEIGKAVRLREGSDITL
                     IASGARMVWEALEAGTQLAVEGIGARVLNIHTLKPLDREAITAAAMETGAVVTVEDHS
                     VIGGLGCAVAEVVLTTHPAPVMRVGLPDCFCETVGTFEEMLPAYGMDARAIVAAAHRA
                     LVLKGRRG"
     gene            complement(6616..7761)
                     /locus_tag="VEL74_13775"
     CDS             complement(6616..7761)
                     /locus_tag="VEL74_13775"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HYG63645.1"
                     /translation="MFTFQLYPRVHYGEGAATKAGAELTRLGGRKALIVTDPGVLAAG
                     VPQPVLDSLAAAGIAHEIFSGVETNPADVHVMAATEAYRAAGADCWIGLGGGSAMDVA
                     KSAAAVVSNGGHIRDYEDGARPIEHPHAPLMFVPTTSGTGSEVVGGTIIIDTSRQFKM
                     HIVAVPGHVALCDPLLTLSLPPAVTAATGLDALSHAIGAYVSVERQPLADAYALYAIE
                     LVQKWLPRAIADGQDREARAWMMVASLTAGISMKGGGSGEHAFAHGVNVQHRVHHGVG
                     CAVFLADVMDYNLPHAADRYARIAQALGIERGASSDLELGRAGIAAVRRLVRDCGIPT
                     LGGLGVGESHVAPLVDYVMQDAFHLGLNPVPITPPDAERILRTTLAG"
     gene            complement(7784..>8283)
                     /locus_tag="VEL74_13780"
     CDS             complement(7784..>8283)
                     /locus_tag="VEL74_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63646.1"
                     /translation="MVLLQDRLAAAVSVPVITSALLMVPQIHRMMPAKRLGILVFHPE
                     SVGEPVYNACGWGEREIPVAVGGVAGSEAWHEFLRTKEIPDRLRPRLEADVIAVGRRM
                     LDEHPDIGAFVCECTLMPPANQALRASLRLPVYDILNLLDLAMRGLFRAPDSRAAPTP
                     DPSAC"
BASE COUNT         1272 a         2895 c         2774 g         1342 t
ORIGIN      
        1 ggtggtggtt gcctgcacgt cgtcgcccgc gagcggctag cggacgccac agccttcgcc
       61 gagttgatgg cgcgccagcc ggtggacgtc ctcaagatcg tcccctctca cctcgcggcc
      121 ctgctcactg cggagagccc cgagcgctcg ctaccccgcc gcctgctcgt cctgggcggc
      181 gagccgctcc cctggagcct ggtggaacga gtgcgctccc tggcgccaga gtgccggctg
      241 ttcaatcatt acggtcccac agaaacgacg gtcggagttc tcgcgggcgc agtggaaatc
      301 cagaggcacg aaatgggtga agcgggaggt actcgcggga gcggtagtac ctgcgctccg
      361 ctcggctggc cgcttggaca aacccgcggc tgggtggtcg atcgccatct ccggctggta
      421 ccggctggcg tgcccggcga actctgtgtc ggcggtccgc aggtgacccg cggctacctc
      481 ggacgtccgg acctgacggc cgaacggttc atccccgacc cgttcggcgg cggcgccggt
      541 gagcgtctct accgtacggg agaccttgtg cgccggcgtc caagcggccc gatcgagttc
      601 ctcggccgca ccgaccacca agtgaaggtc cggggattcc gcatcgagtt gggagagatc
      661 gaagcggcgc tggcgacttt gcccggggtg cgcgaggctc tggtggtggc acgggaggac
      721 acgcccggag aacggcgctt ggtcgcctat gtggccggcg atgtctccga gacagccgtc
      781 gacacgctcc gagcgtcctt gcgggagcaa ctgccggatt acatggtacc agccgccttc
      841 gtgacgctca ccaccctgcc gctcacctct aacggcaaag tggaccgcaa ggctctgccg
      901 gctccggaac cccctcgccc cgcggagagc cactctgcaa tgcagacgcc catcgaagac
      961 gccctcgccg gcatctgggc cgagttcctc ggtctcgagc aggtcggggc gaccgaccac
     1021 ttcttcgacc tgggaggcca ctcgcttctg gcggtacggt tgatgtcccg gatcgagcac
     1081 gtcttcgggg tcaagctacc gatctcgaca ctgttcgagg cgccgaccgt ggagcggttg
     1141 gccggggtgc tcctccgggg gtcggtaccg gcccgtcact cgcctctggt acggctccac
     1201 acgggcggca cgggccgccc cttgttcctc gttcatccga tcggaggtga cgtctcatcg
     1261 tacgtggaac tggctcgccg actcggggcc gaccggccgg tgtacgctct gcagccggtc
     1321 accgtcgagc caggtgctga gagagctgcc ccgtctacca tggaagagat ggccaggcag
     1381 tatctcgccg ccatgcgcca ggttcaggct ggaggaccct ggttgatagc cggctggtcc
     1441 ttcggcgcga tcctcgccta cgaaatggca caacagatcg aaagctcggg cgataccgtg
     1501 gcactcctgg cgatgatcga tccctcggca cctccggaag cgcgcaacga aagggtcgac
     1561 gatatccagt tgctggccgg tttcgcgggc ctcgggcgcc cttcggagcg gcagagggaa
     1621 ttgctacggg agatgctggc ggaagtcgac gtggaggccg ggctcgatcg tctgctcgaa
     1681 ttcgggcagg ccgagggagt gctgccgcca gacgtgggca agccctggct gcgggagcgt
     1741 ttcgacctgt tcagccgcaa cgtgcaggtt ttgcgcggtt acgtggcacg cccctacggc
     1801 ggtcgggtca cctttttccg agcgggcgcc tcgctggctc aggggacgat ggagctcacg
     1861 tcaggctgga gcacgcttgc tcataccgag gcacacctcg tcgattcgga tcacgtctcc
     1921 atgcttcgca ggccagctct cgaccacctg gtagagcaac tttccacgga cctcgcggcg
     1981 gtcgaggccg gagccgaagc gccatgaagg agctcattca ggaccttcgt ttttcgtttc
     2041 gcctgttccg caagagtccg ggcttcacgg ctgcggtggt gctgacgctg ggcttgggca
     2101 tcggcgtcaa tgtggcaatc ttcagcgtcg tcgatagcgt cctggtgcgg ccgttgccgt
     2161 tccgtgacgg ccatcagctt gtccatcttc gctatggcaa acccaacctc gggccggaca
     2221 ccatccgctt ctcggtgcca gaatcacacg actaccgccg ccacagccgg acgctggccg
     2281 ggctcaggga ataccacacg atgtccttca ccctgctcgg cggagaagag ccgaaccagg
     2341 tcctggtcgg cgtggtttcg gccaacttct gtgatgtcct ggggattaag ccgatgctcg
     2401 ggcgggcctt cgttccggag gacgaacagc cgggtgcccc gtccgtcttg ctcctcagca
     2461 accggtactg gcggagccgc ttcggcggga accccaacat cgtcggccgg gccctgcgga
     2521 tgaatggacg ctccatccaa gtcgtcggag tcctcccgcc gttgcccgac ttcccgcgcg
     2581 ccgacgatgt cttcgtcccc acctcggcct gccccttccg ctccaacccg gaggttgtcg
     2641 aagggcggga caccaggatg atcaatcttt tcggccgcat gcgacagggg gtcaccctag
     2701 agcaggcccg agccgagatg tcgctcctgg ccaaaccgcc agcggagtga ctatccggac
     2761 tccttcccgg aagaggcgcg tgccaccctc tccctggttc cggtttggga cgaattgaac
     2821 ggtaagttcc tcccgacgct ccagatcctg ctcgtcaccg tcgtcctgct gctcctcatc
     2881 acctgcgcca acgtgggtaa cctttctctc gcccgcctgt tgagccgcga gcgcgaggtg
     2941 gtgctgcgtg ccgcgctggg agccggccgg ctccgcctga tccgccaact cctaaccgag
     3001 agcacggccc tggctctgat agggggcgct gtcggcctcc tcttcgccgc ggtcaccatg
     3061 agctccctga cggccttcgc ccggcgcttc acccctctcg ccaaccggat cgacatcaac
     3121 ggccgggtgc tgctcttcac cctggggatc gccgtcctct ccggtctcct cttcgggctt
     3181 ttccccaccc tccacgccta caggcgaaac ctgggagccc tcctgcgtca aggtggcggc
     3241 cactccaccc atgaccgcgg acggcagcgc ctgcgcagca cactggtcgt cctccaggtg
     3301 gccgtctcct tcgtcctgct gatcgcggcg gggctgacta cgagaagcgc catccggcta
     3361 cagagggtcg acggcggctt cgatcccagg aacgtggtcg cgctcagcct cgaactgccg
     3421 tcggcgagat aacccgggcg gcaggaggtg ctcgacttct acaactccct gttggcgcgg
     3481 gtcggcggtc tgccgggagt ggtgtcggcc gccgtctcga actccgcgcc actgcttgcg
     3541 gatgagtcgg cctccacctt tcggatcgaa ggccgtactc agcaggcgga tggcaccgag
     3601 ccccgggccg ccgggcgggt cgtgagccag gacttcttcc gcacacttgg catcccttgc
     3661 tcgccggccg ggcgtttctc gcatcggacg acgcaaacgg tctgccggtc accatcatca
     3721 accagtccct ggccagacgt ttctggcccg ctgagagccc tctcggcaaa cggatcgaca
     3781 tcggggagac cggggcgccg atctggctga cggtggtcgg tatcgtcggt gatgtccacc
     3841 acgccggcct cgatgacgcg gccgaggacg ccttctatgt ccccttcctg cagtatggcg
     3901 gggtggcgat gaacttgttt gtccgcacca ccggcgagcc gatcctcctc cccccggcgt
     3961 tgcgcaaggc cgtccacgag atcgatcccg agcaatcgat cagcgacatc tcaatgatgg
     4021 agcagatacg ctccgattcc atcgccccca tgcggctgac cgcgacgctg ctcagcctcc
     4081 ttggactcct ggccttcgcc atcacggtgg ccggcctgaa cggcgtgatc gcctattcca
     4141 cgaccgagca gacgcaggag atcggtatcc gcgcggcgct cggcgcgaga cgcacggtcc
     4201 tgcttcatct ggtaatgcgc cagggcatgg ccctggtgct actcgggctc gcgctgggct
     4261 gggccggagc cctcggcggc ggcaaattgc tggcgagcag gctgttcggt gtcgagccgt
     4321 ccgatcccgt caccttcctg ctggcatccc tggcattgct cgtctgcggc ggcgccgcct
     4381 gcttcttccc cgcgcgaagg gcagccttca ccgacccaat ggtggccctg cgatcctgag
     4441 cctcggaagc gtccggctac gcccaggccg cgcggtagac cgcgagcagg tcgttccggt
     4501 cgagggcaac gggggtattc tcgaggaccg gccggaagcg ggtcgcctcg tcagcgaggg
     4561 ctggcaggtc ctcctcgcgc acgcccaccc cggcgaggcc gcggtccacc ccaagcgttt
     4621 cgaggagatg gcggacgcgc ccggcgaggg tgtcttcgcc gtcgcccagc agccgggcga
     4681 gttccgcgca ccgcccggga ttaccggttc cgatccgttc cagagcggga gccgccatca
     4741 gggcgttggc caggccgtgg gggatgccgt agcggccggt cagcggatag cccagggcgt
     4801 ggatggcgcc cgcgttggca ttggccatcg ccaagccgga gtagagacaa ccgagggaga
     4861 gttcctcgca cgccgcgaag tccgaacggt cggccagcac cctggggaga gcgcgggcga
     4921 tggccggcgc cgcctgccgg gcgaacatgt cggtaaacgg cgtggcacgg cgggcgagga
     4981 aggcctccac cgcgtggatg aaaccgtcga gagcgctcca ggtgaggtgg gaggagggga
     5041 gggtcacgat cagctcgggg tccacgaggg tcgccagggg cagcagatgg agacctgcga
     5101 tcacttcttt cctgcgggga gagagttgta ccagcacggc atggctggag acctcggcgc
     5161 cgcttcccgg ggtcgtcggc accgcgagga ggggcaggct cggatgccgt accttctcga
     5221 agccgaagta atcggcggtc ttcccgccat tggtcgccaa gactgcggcc accttggcaa
     5281 catcgatcac gctgccacca ccgaaggcga tgacggagcg atgaccgccc gcggcgatcc
     5341 gttccgcgca acgcgccgcg tgcccctcgt ccggatcctg tgccacgcca tcccagacct
     5401 cggcctcgga tcccgctccg gcgagctcgc ccagcaggcg ctcgaccagc cccgccgaca
     5461 ccaccccggg gtcggtgacc accagaggcc gctccgcccc cagctctccg aggaccgccg
     5521 gcacgtcggc catgcggccc cgcccgaaga gcagcgtggg cgggaccctg accttcatcc
     5581 ccgccggccc ttgagcacca gggcgcgatg ggccgccgcg acgatcgccc gagcgtccat
     5641 gccgtaggcg gggagcatct cctcgaaggt gccgaccgtc tcacagaaac agtcgggcaa
     5701 ccccacccgc atgaccggcg ccggatgggt cgtgagcacc acctccgcca ccgcacaccc
     5761 caggccgccg atcacgctgt ggtcctccac cgtcaccacc gcccccgtct ccatggcagc
     5821 ggcggtgatc gcctcgcggt ccagcggctt caaggtatgg atgttgagca cccgcgcgcc
     5881 gatcccttcc acggcgagtt gcgtgcccgc ctccagcgcc tcccagacca tgcgggcgcc
     5941 ggaggcgatc agggtgatgt cggagccttc ccgcagccgc accgccttgc cgatctcgaa
     6001 ctcatagtcg ccgaagtaga cctgcggtgt ctcggccctc ccgacccgca ggtagaccgg
     6061 cccctcgtgg gcgtaggcgc cccaggtcgc cttccaggtc tccaccgagt cggccgggga
     6121 gagcaggacg acgttaggca tggcgcgcac cacagccatg tcctcatacg aatgatgggt
     6181 ccccgccgca gcgcctcccg agatcccagc ataggaaccc accagcttga cgttggaacg
     6241 gtggtaggcc acctccaggc gaacctgctc gcaggcgcgg aaggccaaga aggcggcgaa
     6301 gctgttgacg aacgggatct tgccccgcat cgccagaccg gccgccgtgc ccaccatgtc
     6361 ggcctcggcg attcccatct gcaggaagcg tcgcgggaac tcggcatgga aggggaccgt
     6421 gtattggccc agatccccct cgatagaaaa gacccgctcg tcggcgcgcg cgatgtcgag
     6481 ctgcgccttg cggcaggcgg tgcgggtcga cagggagtac tcggcgatcc actccggcga
     6541 gccgatgacc ggtcccgcct cgcgctcctc ctccgccgcc gttgcgatcg atgaatgcgc
     6601 catgatcgtc ctccgtcacc cggcgagcgt ggtgcgcagg atccgctcgg cgtccggagg
     6661 ggtgatgggc accgggttga gaccgagatg gaaggcgtcc tgcatcacgt agtcgaccag
     6721 cggcgccacg tgcgactcgc cgacgcccag gccgccgagg gtcgggatgc cgcagtcgcg
     6781 aaccagtctg cgaacggcgg cgatgcccgc ccggccgagc tcaaggtcgc tgctggcgcc
     6841 gcgctcaata cccagcgctt gggcgatacg cgcgtagcgg tcggccgcgt gcggcaggtt
     6901 gtaatccatc acgtcggcca ggaaaacggc gcagccaacc ccgtggtgga cccggtgctg
     6961 gacgttgacg ccgtgggcga aagcgtgctc gccgctccct ccgcccttca tcgagatgcc
     7021 ggcggtgagg ctcgccacca tcatccaggc ccgcgcctcg cggtcctgcc cgtcggcgat
     7081 agcccgcgga agccacttct ggaccagctc gatggcatag agggcgtagg cgtcggcgag
     7141 cggctggcgc tccaccgaga cataggcgcc gatggcgtgg gagagcgcgt cgaggccggt
     7201 ggccgcggtg accgcgggtg gcaggctcag ggtgagcagg ggatcgcaca gcgccacgtg
     7261 cccgggcacc gccacgatgt gcatcttgaa ctgccgcgag gtgtcgatga tgatcgtgcc
     7321 gcccaccacc tcgctgccgg tgcccgacgt cgtggggacg aacatcagcg gcgcgtgcgg
     7381 gtgctcgatg ggccgcgcgc cgtcctcgta gtcgcggatg tggccgccgt tgcttaccac
     7441 cgccgccgcc gacttggcga cgtccatggc gctgccgccg cccaggccga tccagcagtc
     7501 ggccccggcc gcgcggtagg cctcggtggc cgccatcacg tgcacatcgg ccggattggt
     7561 ctcgacgccg gaaaagatct cgtgagcgat ccccgccgcc gcgagggagt cgaggaccgg
     7621 ctggggcacg ccggcggcca gaaccccggg atcggtgacg atcaaggcct tcctcccgcc
     7681 gaggcgggtc agctcggcgc cggctttcgt cgcggcgccc tcgccgtagt ggacgcgggg
     7741 atagagctgg aacgtgaaca tctgcacggg aacaacctcg ctttcaacag gcgctgggat
     7801 caggggttgg ggcggcgcgg gaatcgggag cccggaagag gccccgcatc gctaggtcga
     7861 gaaggttcaa gatgtcgtag acaggcaggc gcaggctcgc gcgtagcgcc tgattggcgg
     7921 gcggcatcag cgtgcactcg cagacgaagg cgccgatgtc ggggtgttcg tcgagcatcc
     7981 gccggcccac ggcgatcacg tcggcctcca gccgggggcg cagccggtcc gggatctcct
     8041 tggtgcgcag gaactcgtgc caggcctccg agcccgccac gccgcccacc gccaccggga
     8101 tttcccgctc gccccagccg cacgcgttgt agaccggctc ccccaccgac tcggggtgga
     8161 acaccaggat gccgagacgc ttcgccggca tcatgcggtg aatctgcggc accatcagca
     8221 gcgccgaggt gatcaccggc accgacacgg cggcggccaa ccggtcctgc agcaggacca
     8281 tga
//
LOCUS       DASPJI010000508         3076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_31153, whole genome shotgun sequence.
ACCESSION   DASPJI010000508 DASPJI010000000
VERSION     DASPJI010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31153"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VEL74_13785"
     CDS             complement(<1..1290)
                     /locus_tag="VEL74_13785"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HYG63647.1"
                     /translation="MSRTLREALRRLAPPVARRLALLLTAVALAGPLSSSALAQTPEV
                     IPEVTPEAISEAAPDAPPCPPIEETAAAAGSQPPCAPAPEVSPSEEAPEPPHPPDGTL
                     IGEVIIIPKGIFDPNEPGENKKIFRFADRLHRATRPKVIERQLLFKSGDAYSREAVEE
                     SERILRANRYLYDADIRPVRQEDGRVDLEVVTRDVWTLRAGASLNRAGGENSTNFSLE
                     DSNFLGTGKDLTIWRISNVDRTSTLFRFRDPHLYGQHLQLEASYADYSDGGSRRFELE
                     RPFYSLDARWTAGFKSWSFDRVDSLYDRGEVFQKFDHGHDFLEIFGGLSPGLQDGATR
                     RLRIGFTHQKDTFNYSPGGDSTPEPPQSRTLAYPWIGFDYIEDGFVVARDLDRIQRTE
                     DLNLGRQFQSRIGWTSPSFGGDENQAIGSASFTAGWSP"
     gene            1362..2228
                     /locus_tag="VEL74_13790"
     CDS             1362..2228
                     /locus_tag="VEL74_13790"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYG63648.1"
                     /translation="MTVSLGALLIVLASSVAWAGVDTSRKMLAGRVRPVPLIVLVTLA
                     SVPPFGAWLAWQGMPEVGGGYWWPALTSLALNVFSNLAFIHALRLSPLSVTVPLLSLT
                     PVFTAVIAIPLLGERPAPLQLAGILLVVTGAFVLHWPGKGRGEGKGRIERGALWVVAV
                     AFSWSLTIPLDKLAVESSNAPFHATALCGGVGLAVLAILIGQGRLRELADVGRARGAF
                     ALCLGGSVLALALQFIAFQLVWVSLIETIKRGVGNLSALLMGRFLFGEPITPRKVLAL
                     GIMAVGVALVVG"
     gene            2495..>3076
                     /locus_tag="VEL74_13795"
     CDS             2495..>3076
                     /locus_tag="VEL74_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG63649.1"
                     /translation="MAKEVGRSVSLAVRRLSQSPSLTLAVVLTLALGFGANITIFSLV
                     DTVLFRPLPLPDADRLVRLIPRVPEMPTLNVSSYPVYTDYREQARSFSALAAFSDIRR
                     VHVARGGEVPERLEAMLVTGSFFSVLEARPLVGTLLSPADDAAGAPAGVVLSESLWRR
                     KFNADLRIVGGSVLINQHPFAVLGVVPGSFVGPV"
BASE COUNT          421 a          992 c         1131 g          532 t
ORIGIN      
        1 cgggctccag ccggcggtga acgaggccga gccgatggcc tggttctcgt cgccgccgaa
       61 ggagggggag gtccagccga tgcggctctg gaactgccgg cccaggttca ggtcctcggt
      121 gcgctggatg cggtcgaggt cgcgggcgac cacgaagcca tcctcgatgt agtcgaagcc
      181 gatccaggga taggcgaggg tgcggctctg cggcggctcg ggggtcgagt cgccgccggg
      241 ggagtagttg aaggtgtcct tctggtgtgt gaaaccgatc cggagccgcc gcgtggcgcc
      301 gtcttggaga cccggcgaga gcccgccgaa gatctccagg aaatcgtggc cgtgatcgaa
      361 cttctggaac acctcgccgc ggtcgtagag cgagtccacc cggtcaaagc tccaggactt
      421 gaagcccgcg gtccagcggg cgtccaggga gtaaaacgga cgctcgagct cgaaccgccg
      481 cgagccgccg tcgctgtagt cggcgtagga ggcctcgagc tggaggtgct ggccgtagag
      541 atggggatcc cggaagcgga acagggtcga ggtccggtcg acgttggaga tccgccagat
      601 cgtcaggtcc ttgccggtcc ccaggaagtt ggagtcctcg agcgagaaat tggtggagtt
      661 ctcgccgccg gcgcggttga ggctcgcccc ggcccgcagg gtccagacgt cgcgggtgac
      721 cacctccagg tccacgcggc cgtcttcctg gcgcaccggc cggatgtcgg cgtcatagag
      781 gtaacggttg gcgcgcagga tgcgctcgga ctcctcgacc gcctcccggg agtaggcatc
      841 gccgctcttg aagaggagct ggcgctcgat gaccttgggc cgggtggcgc ggtggagccg
      901 gtcggcgaac cggaagattt tcttgttctc tcccggctcg ttcggatcga agatcccctt
      961 ggggataatg atgacctcgc cgatcagggt gccgtctggc ggatgcggcg gctcgggcgc
     1021 ctcctcactg ggagagacct cgggagcggg ggcgcagggc ggctggctgc cggccgccgc
     1081 cgccgtctct tcgatgggag gacagggggg agcgtccggc gccgcctcgg agatggcctc
     1141 cggagtgacc tccgggatga cctccggcgt ctgggccaga gcactggacg acagggggcc
     1201 ggccagcgct acggcggtga gcaggagcgc caggcggcgg gcgacagggg gggcgaggcg
     1261 gcgcaaggcc tcgcgcaggg tacggctcac aggccctccg gcggacgtgg ctgaaagttc
     1321 cccggcatca taacaaagtc cgggtatgat gctcgccccc tatgaccgtc tccctcggag
     1381 ccctgctgat cgtcctcgcc tccagcgtcg cctgggcggg ggtggacacc tcccgcaaga
     1441 tgctggcggg gcgggtccgg ccggtgccgc tgatcgttct cgtcactctg gcctcggtgc
     1501 cccccttcgg cgcctggctc gcctggcagg ggatgccgga ggtcggcggg ggctactggt
     1561 ggccggccct gacgtcgctg gcgctcaacg tcttctccaa cctggccttc attcacgcgc
     1621 ttcggctctc gccgctcagc gtgacggtgc cgctcctctc cctgacgccg gtcttcacgg
     1681 ccgtcatcgc tatccctctg ctaggggaga ggccggcgcc gctccaactc gccggcatcc
     1741 tgctggtggt gaccggcgcc ttcgtgctcc actggccggg gaagggccgg ggagagggga
     1801 aggggaggat cgagcgcggc gccctctggg tggtggcggt cgccttctcc tggtcgctga
     1861 cgatcccgct ggacaagctg gcggtcgaga gctcgaacgc cccgttccat gccacggccc
     1921 tctgtggcgg ggtgggcttg gcggtgctgg cgatcctgat cgggcaggga aggctgcgcg
     1981 agctcgccga cgtcggccgg gcgcgcggcg ccttcgccct ctgcctgggg ggcagcgtgc
     2041 tcgcgctggc gctccagttc atcgccttcc agctcgtctg ggtcagcctc atcgagacca
     2101 tcaagcgcgg ggtggggaac ctctccgcgc tgctcatggg gcgcttcctc ttcggcgagc
     2161 cgatcacccc gcgcaaggtc ctcgccctgg ggatcatggc ggtgggcgtg gcgctggtcg
     2221 tggggtaggg cgacaggccc gcggccacgc cggcagctcg caagacttgc gatagctcaa
     2281 gatctactgg tatactgcca ttgctaaaag ggagcgcacc cgacggctga taacgacagc
     2341 ggtcgggttt tctaatcgtc ccctcggcaa aggcgtggga gcctggccga ctctttgggg
     2401 atagggcgag agggccggcg gtggagcttc tgaccaccgc gccccgcgct gtgcgacgag
     2461 cggagagacg agcggagaat cgatgcgaag ggacatggcg aaggaagtag ggcgttccgt
     2521 ttcactagcc gtccgtcgtt tgagccagag ccccagcctg acgttggcag tggtgctcac
     2581 cctggctctc ggtttcggcg cgaacataac gatcttcagt ctcgtggaca ccgtgctgtt
     2641 ccggcctctg cccctgccgg atgccgaccg gctcgtccgc ctcatcccgc gggtcccgga
     2701 gatgcccact ctcaacgtct cctcgtatcc ggtctacacc gactatcgcg agcaggcgcg
     2761 ttcgttttcc gcgctggcgg ccttctcgga tatccggcgc gtgcacgtcg cgcgcggcgg
     2821 cgaggttccc gagcgcctgg aagccatgct ggtcacgggg agcttcttca gcgtgctgga
     2881 ggcccggcct ctggtgggca cgctgctctc tccggccgat gacgccgccg gggcgccggc
     2941 cggcgtggtg ctgagcgagt ccctctggcg acggaagttc aatgccgatc tgcggatcgt
     3001 gggtgggagc gtcctcatca accagcaccc gttcgcggtg ctcggcgtcg tcccgggcag
     3061 ctttgtgggg ccggtc
//
LOCUS       DASPJI010000509         8046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_91129, whole genome shotgun sequence.
ACCESSION   DASPJI010000509 DASPJI010000000
VERSION     DASPJI010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8046)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91129"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VEL74_13800"
     CDS             complement(<1..1346)
                     /locus_tag="VEL74_13800"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HYG63650.1"
                     /translation="MKRLSYLWPYLRSHRRKLVQGLLAILASVALGLMSPLILGRAID
                     DLRTGVSRETLLAYAGLILGITLFQGLFNYLQRMILVSMSRDIELDLRDLYFSSLEAQ
                     PPAFFHDRPTGDLMARATNDLQAVRMLCGPAIMYSSNTVFTAAGALFFMLRIDVRLTL
                     LALVTMPVVAVVTNVFGQRIHALFERIQAQFSTVSARVQENLSGVRVVRAYAQEEREE
                     AEFRRLNAEYVAGNRRLIGWTVAFHPLLQGLVGLGFAAVLWYGGGLVIRGGITVGQFV
                     TFNFFLGKLIWPMIAVGWVINLAQRGLASLGRMREVMDVVPAIRDEEPLVGLPEVAGA
                     VRAADLTFSYRLDTPPVLSGIDFQAAPGDTVALVGRTGSGKSTLLSLLPRLFDPPEES
                     LRIDGVDVRRLPLARLRAAIGMVPQETFLFSATIRENIALGRPEASREEVAEAARLA"
     gene            complement(1343..2083)
                     /locus_tag="VEL74_13805"
     CDS             complement(1343..2083)
                     /locus_tag="VEL74_13805"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HYG63651.1"
                     /translation="MIQPALVSTIDGDRSLSDFPAPLPLDVLVPETMPGAGEWEVEIG
                     FGKGRYLLRRCLEDPQRRFLGIELVGEYQRLFVDRARRRGARNWVAMRGDALFLMSSV
                     LPPGFAAAVHVYFPDPWPKSRHHKRRLFDPQTVDLVLGLLADGGRLFFATDFLEYGEL
                     VTEILESFPGLDVQRRDAPWDEGPRTNYEAKYMVEGRPILRLVATPRSAPPLHPQGAA
                     GVLAAPALTGTPGEAGEADILPVGATPS"
     gene            complement(2080..3132)
                     /locus_tag="VEL74_13810"
     CDS             complement(2080..3132)
                     /locus_tag="VEL74_13810"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HYG63652.1"
                     /translation="MQALILAGGSGTRFWPLSRKRRPKQLLALEGERSLLRDTVDRLQ
                     PLVPPSAVWVCTTRALADEVRRELPEVPAAQILLEPEGRNTAPAIGWSVRSMPEEARR
                     GVVAVLPADHRFGDPAAFRDTLERAREVVEREDRVMTLGVTPRWAETGYGYLELEPAG
                     DVDGPEGVRRLRRFVEKPDAEAAARFVSSGNYLWNAGIFVFRGATLLRVLADLQPELA
                     RGLEEIAAAPERLDETYARLPSDSIDYAVMEKLSDISTLPLDCGWSDLGSWAALDEIL
                     PRDASGNTGRGERIALDSSGNLLFSDAGTIAVVGVRDLVVVRTGDTVLVCPKERSQDV
                     KRIVTELAARGREDLL"
     gene            3335..4600
                     /locus_tag="VEL74_13815"
     CDS             3335..4600
                     /locus_tag="VEL74_13815"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63653.1"
                     /translation="MKIHPNEEALQDLFRQQDRGTLRMLEHVLGCPECRERAQGVLAS
                     WRHSGAGKLLKLPAAAPDYDRALDNATRGLLARTICIERERAEARALLPTLESCPPER
                     RKLLLLNSARYQTWGMLELLLEQSRTADFQDSQRIEHLSELALVLADQLRPESYGEPS
                     LEDMRARAWSYIGNARRLQFDFKGAEEAFATAFHHLRRGTGDPVERALLLSFKASLCR
                     VQGKKSQAVQLIHRAIDIFRTAGESHKAGKCLVQLAGVHLNSGTPEEAIPLLYEALEL
                     LGDSAEPQLSFFAWHNLISGLTCTGRYLEAQSALRRTKDLYDRASLPWAGVRLRWMEG
                     KIALGLGQVQEAEGALLEARDGFLAAGAVYEAAQVTLVLAALYHKEGRPAAIRPLAEE
                     IAGFVDRPGKAREAQEALALLRTAESGIA"
     gene            5597..6118
                     /locus_tag="VEL74_13820"
     CDS             5597..6118
                     /locus_tag="VEL74_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63654.1"
                     /translation="MINLDGKGFRFTSLEEGVRFDIDADGELERLAWTAPNGRDAFLV
                     LDRNGNGRIDDGAELFGGATPQPASEEPNGFATLAVFDEAASGGNGDGVISVSDAVYE
                     RLGLWIDSNHDAVSQPYEVTPLSSEGIQAIDLTPVVSQRRDRHGNRSKWASHVLFLHS
                     LRLAAVDVIFLAE"
     gene            complement(6130..6477)
                     /locus_tag="VEL74_13825"
     CDS             complement(6130..6477)
                     /locus_tag="VEL74_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63655.1"
                     /translation="MAAGLGWTQEALGHLEAVREEFLRRNIAYDTALVTLEIAGLYLL
                     EGRTAEVKALACELVTLFQAQKVHRETLAAVQLFHKAAERETVTREAVRQLLTYLQRA
                     RSEPGLKLDTLAG"
     gene            complement(6504..>8046)
                     /locus_tag="VEL74_13830"
     CDS             complement(6504..>8046)
                     /locus_tag="VEL74_13830"
                     /inference="COORDINATES: protein motif:HMM:NF042672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6624 domain-containing protein"
                     /protein_id="HYG63656.1"
                     /translation="KPAPLMLTWRGQRWDAFGATAEKLAEPDPMALDVRRALRLGNTT
                     DGRLWAAFAYRHQFHEYSSSGRLLTEIVVDGGDVHEVEDAEARRQQGANYQKALAEQG
                     IRSQVMEMTARPMGIALSEGRDGRMYFLVHGDSGTAGSQVSLERFDPARAVLERMWLP
                     IPIPGRASMASGWDGLYVAPSRAQHGLWRIPWEDLEAAEWEPVQGVEIGISDPSKLEG
                     VWREKGGRGLVRFAKDKVITWEDGRLTVRGLVSLEGREALLRRSGFGESWKVALRSEG
                     LRIERDGGGREYTRLSEIPKPVSLDPAPLGKVKALAPERVRTVQSELEQRTLNAQKAR
                     ESKDPNQRLAVDADNIDFLRRLTAEVGWIDAGRFGVTAASQAAVLAQNSLNVLLMLAA
                     LPKVEKDLKDLPEGGPVFAAFYDALQVALGGKQRYGTQVVPDAEGDPVVLPLERRAEV
                     DAARRKLGLPSLPEALALLSREVFDGKPVRIAEGEVVQAPPAAARQAGTRPAAPSPLA
                     GVPGT"
BASE COUNT         1314 a         2692 c         2842 g         1198 t
ORIGIN      
        1 gccaggcgcg ccgcctcggc gacctcctcc cggctggcct ccggccggcc aagcgcgatg
       61 ttctcgcgga tggtggcgga gaacaggaac gtctcctggg gcaccatgcc gatggcggcg
      121 cgcagccgcg ccagggggag ccgccggacg tcgacgccgt cgatccgcag gctctcctcc
      181 ggcggatcga agagccgcgg gaggagggag agcagcgtgc tcttgcccga gccggtccgg
      241 cccaccagcg ccacggtgtc gcccggcgcc gcctggaagt cgatgccgga gaggaccggc
      301 ggggtgtcga ggcgatagga gaaggtgaga tcggcggcgc ggacggcgcc cgccacctcg
      361 gggaggccga ccagcggctc ctcgtcgcgg atggccggca ccacgtccat cacctcgcgc
      421 atgcggccga gggaggccag gccgcgctgc gccaggttga tcacccagcc gaccgcgatc
      481 atcggccaga tgagcttgcc gaggaagaag ttgaaggtga cgaactggcc gacggtgatg
      541 ccgccgcgga tcaccaggcc gccgccgtac cagagcaccg cggcgaagcc gagccccacc
      601 agcccctgga gcagggggtg gaaggcgacg gtccagccga tgagccgccg gttgccggcg
      661 acgtactcgg cgttgaggcg ccggaactcg gcctcctccc gctcctcctg ggcataggcg
      721 cggaccaccc gcacgccgga caggttctcc tggacgcggg cggagacggt ggagaactgc
      781 gcctggatgc gctcgaacag ggcgtggatc cgctgcccga agacgttggt gaccacggcc
      841 acgaccggca tggtgacaag ggcgagcagc gtcaggcgga cgtcgatccg cagcatgaag
      901 aacagcgccc cggcggcggt gaacaccgtg ttgctgctgt acatgatggc cgggccgcag
      961 agcatccgca ccgcctgcag gtcgttggtg gcgcgggcca tcaggtcgcc cgtggggcgg
     1021 tcgtggaaga acgccggggg ctgggcctcg aggctcgaga agtagaggtc gcgcaggtcg
     1081 agctcgatgt cccggctcat gctcaccagg atcatccgct gcagatagtt gaacaggccc
     1141 tggaacaggg tgatgccgag gatgagcccg gcataggcca gcagggtctc gcggctgacg
     1201 ccggtccgca ggtcgtcgat cgcgcgtccg aggatgagcg ggctcatcag ccccagggcc
     1261 acggaggcga ggatcgcgag gaggccctgg accagcttgc ggcggtggct gcggagatag
     1321 ggccagagat aggaaagccg cttcaagacg gagtcgctcc caccggaagg atatcagcct
     1381 cgccagcctc cccgggcgtg ccggtgagcg caggggcggc cagtacgccg gccgcgcctt
     1441 gcgggtggag cggcggggcg ctccgcggcg tcgccacgag gcgcaggatg gggcgcccct
     1501 cgaccatgta cttggcctcg tagttggtgc ggggcccctc gtcccagggg gcgtcgcggc
     1561 gctgcacgtc gaggccgggg aagctctcca ggatctccgt caccagctcg ccgtactcca
     1621 ggaagtcggt ggcgaagaag agccggccgc cgtcggcgag gaggccgagc accaggtcga
     1681 ccgtctgcgg gtcgaagagg cggcgcttgt ggtggcggct cttgggccag gggtccggga
     1741 agtagacgtg gacggcggcg gcgaagccgg gggggagcac ggaggacatg aggaacaggg
     1801 cgtcgccgcg catcgccacc cagttgcggg ccccccggcg ccgggcgcgg tcgacgaaga
     1861 ggcgctggta ctcccccacc agctcgatcc cgaggaaacg gcgttgcggg tcctccaggc
     1921 agcggcggag gagatagcgc cccttgccga agccgatctc cacctcccac tcccccgctc
     1981 ccggcattgt ctccgggacg aggacgtcca gcgggagagg ggccgggaag tcggagagcg
     2041 aacggtcgcc gtcgatggtc gagaccaggg cgggctggat caaagcaggt cctcgcgtcc
     2101 gcgggcggcg agctcggtga cgatcctctt gacgtcctgg gagcgctcct tggggcagac
     2161 cagcacggtg tcgccggtcc gcaccaccac caggtcccgg acgccgacca cggcgatcgt
     2221 gcccgcgtcg gagaacagga ggttgccgct ggagtcgagg gcgatccgct cgccgcggcc
     2281 ggtgttgccg gaggcgtccc gcggcaggat ctcgtcgagg gcggcccagg agccgaggtc
     2341 gctccagccg cagtccagcg gcagcgtcga gatgtccgac agcttctcca tgaccgcgta
     2401 gtcgatggag tcggagggga ggcgggcgta ggtctcgtcg agccgctccg gcgccgcggc
     2461 gatctcctcg aggccgcggg cgagctccgg ctggaggtcg gccaggacgc ggagcagggt
     2521 ggccccccgg aagacgaaga tgccggcgtt ccacaggtag ttgcccgagg agacgaagcg
     2581 ggcggcggcc tcggcatcgg gcttctcgac gaaacggcgg aggcggcgca cgccctccgg
     2641 cccgtcgacg tccccggcgg gctccagctc gaggtacccg tagccggtct cggcccagcg
     2701 cggggtgacg ccgagggtca tgacgcggtc ctcgcgctcg acgacctcgc gggcgcgctc
     2761 cagggtgtca cggaaggcgg cggggtcgcc gaagcggtga tcggcgggga gcaccgcgac
     2821 gacgccgcgc cgcgcctcct ccggcatgga gcgcaccgac cagccgatgg cgggggcggt
     2881 gttgcgcccc tcgggctcga ggaggatctg cgccgccggc acctcgggca gctcgcggcg
     2941 gacctcgtcg gcgagcgccc gggtggtgca cacccagacg gccgaaggag gcacgagggg
     3001 ctggaggcgg tcgacggtat cgcgcagcag gctgcgctcg ccctccaggg ccaggagctg
     3061 cttgggccgc cgtttgcggg agagcggcca gaagcgggtg ccgctgccgc cagcgaggat
     3121 cagtgcttgc atgagagaac cctctttcgc atccgcggga gtatatccgg caggtcccgg
     3181 gctgtcgccc ctggccccct cctgcggctc gttgcctggg aaacgacctt ttttgtcacg
     3241 ccggacccta cggcgggaac attacttatg aggtcttccc cggtccgggg ccgttcggac
     3301 cggattcttt tgttttccga agtgaggtcg tacgatgaaa attcacccga acgaggaggc
     3361 tctgcaggat ctcttcagac aacaggatcg tgggacgctt cgcatgctgg agcacgtgct
     3421 ggggtgtccg gaatgccggg aacgggcgca gggggtgctg gcgagttggc ggcattcagg
     3481 agccggcaag ctgctgaagc tgccggcggc ggccccggac tacgatcggg ccctcgacaa
     3541 tgcgacgaga ggccttctgg cgcggacgat ctgcatcgag cgggaaaggg cggaggccag
     3601 ggcgctcctt cccacattgg agagctgtcc acccgagcga agaaagctcc tgttgctcaa
     3661 cagcgcgcgc tatcagacgt ggggaatgct ggagcttctg ctcgagcaga gccggacggc
     3721 cgacttccag gactctcagc ggatcgagca cctttcggag ctggcgctgg tcctggcgga
     3781 ccaactccgc cctgagtcgt atggggagcc cagcctggag gacatgcgtg cccgtgcctg
     3841 gtcctacatc ggcaacgcac ggcggctcca attcgacttc aagggcgcgg aggaggcgtt
     3901 cgcaacggcg ttccatcacc tgcgccgagg gacgggagac cccgtcgagc gggccctcct
     3961 gctgagcttc aaggcctccc tctgtagagt gcagggaaag aagtcgcagg ccgtccagct
     4021 catccaccgc gccatcgaca tcttccgcac ggccggcgag tcgcacaagg cggggaaatg
     4081 cctggtccaa ctcgccggcg ttcacctcaa ttccggcact ccggaagagg ccatcccctt
     4141 gctctacgag gcactggagc tgctcggcga ctcggccgag ccccaactct cctttttcgc
     4201 ctggcacaac ctcatcagcg gcctgacctg cacggggcgc tatctggagg cccagagcgc
     4261 gctccgccgg accaaagacc tatacgaccg ggcgtctctt ccgtgggccg gggtccgtct
     4321 gaggtggatg gagggaaaga tcgccctggg cctgggccag gttcaggagg ctgagggggc
     4381 cctcctcgaa gcccgggacg gcttcctcgc ggcaggagcg gtctacgaag ccgcgcaggt
     4441 gacgctcgtg ctggcagcgc tctatcacaa ggaaggacgg ccagccgcga tccggccgct
     4501 tgccgaggag atcgccgggt tcgtcgacag gccggggaaa gcccgagagg cacaggaggc
     4561 cctcgccctc ttgcggacgg cagagagcgg gatcgcctag ccgcgggctg aaacgccgtc
     4621 ggaccggtgg tctatcccgc ccgccaggca tgacagacca ccggaccgac gagacagacc
     4681 agcggagcgg gggaacgctc gcccggctgg gcggtccatc cgcggagccg aagccacgcc
     4741 cctctggacg gatgaactgg ccttccgccc ggacagcccc tctctcggga ccggagccct
     4801 ggagggtcga acggaggaaa ccaggctccg ggccgccgtt ccatcctgcg gaccggcgtt
     4861 cgatccccag cggatggatg gaacgagcca acggagtggt gaggcactcc gcctcaccga
     4921 tgaacctgac ctccggacga acggggcatc cagccgagcc aacgccacgg cctaccggga
     4981 tggcaagacg acccgccggg ccgcggccct ctcctccggc cggataggcc aaccagcgga
     5041 gccgatgaag cgacccgccc gaccgagaac ctcgaggtcc ggagggatga gcccctccgc
     5101 cggaggcatg aagcaagcct ctgcgctgat gagccgttgg gacggctcga caggcggaag
     5161 agcccgctct agtgctccag gtctcccttg ccggcaagga cgcgggactc cagccaggag
     5221 accctcacga ggtcgttccg attctcctgc gccgccgcca gcatctagac ggaagcagca
     5281 gcttctccgc ctgaacggcg atgagggttt cgcggtgaat cttccgtcac tctatgctgt
     5341 agaatccgcg tcttcaagga aggaaaaagt cttgacactc aagctcgttc acccgctcct
     5401 ggcagccact ctcggaatcc ttatggcctt ccccgtcacc cctgccattt ggcgagacct
     5461 ctgtcacggt gaaagggacc cataggggcg agatcaccag cttcgattca gccgactcca
     5521 gctatacctg gatcattcag ggaactcttc aagagaaggc aaacttggat gcgagccgga
     5581 ctcttgcacg cccattgtga tcaacctcga cgggaaaggc ttccgcttta ctagcctcga
     5641 ggagggtgtt cgcttcgaca tcgacgcgga tggtgagctt gagcgcctgg cttggacggc
     5701 gcccaatgga cgcgatgcgt tcctggtgct ggaccgcaac ggcaacggcc gcattgatga
     5761 cggagcagag ctgttcggcg gggctactcc acagccggcg tcagaggagc ccaacggctt
     5821 cgcgaccctg gcggtgttcg atgaggcggc ttccggtggc aatggggacg gcgtgatctc
     5881 cgtgtcagac gccgtgtatg agcggctcgg gctatggatc gattcgaacc atgatgcggt
     5941 gtcgcaaccc tacgaggtga cgcccctatc ctcagagggg attcaggcca tcgatctgac
     6001 gcctgtggtc tcccagcggc gcgaccggca tggcaaccgc tcgaagtggg cgagccatgt
     6061 gctcttcctg cacagcctgc gtctcgcggc ggtggacgtc atctttctcg ccgagtagag
     6121 ctgccctcgt catcctgcca gggtatccag cttcagcccc ggctcgctcc gggcgcgctg
     6181 taggtaggtc aagagctggc ggactgcctc gcgggtgacg gtctcccgct cggcggcttt
     6241 gtggaagagc tgaacggcgg cgagggtttc gcggtgaacc ttctgggcct ggaagagggt
     6301 caccagctcg caggccaggg ccttcacctc cgcggtcctc ccctccagca ggtacaggcc
     6361 ggcgatctcc agggtgacca gggcggtgtc gtaggcgatg ttccggcgga ggaactcctc
     6421 ccgcaccgcc tccaggtgcc cgagcgcctc ctgcgtccag cccaggcccg cggccacggt
     6481 gcgttgcagc cagcgggcgg gtctcaggtt cccggcacgc cggcaagtgg agacggggcg
     6541 gcggggcggg tgcctgcctg ccgggcggcg gcaggcggcg cttggaccac ctccccctcg
     6601 gcgatgcgga ccggcttgcc gtcgaagacc tcgcggctca agagggcgag ggcctccggc
     6661 agagacggca ggccgagctt gcggcgggcg gcgtccacct cggcgcgccg ctcgagaggg
     6721 agcacgacag gatcaccctc ggcatccgga acgacctgcg tgccatatcg ctgctttccg
     6781 ccgagggcga cctgcaaggc gtcgtagaag gccgcgaaga ccggcccacc ttccggcaga
     6841 tccttcaggt ccttctcgac cttcggcaac gccgcgagca tcaggaggac gttgagactg
     6901 ttctgggcca gaaccgcggc ctgagaggcg gccgtgactc cgaaccggcc ggcgtcgatc
     6961 cagcccacct cggcggtcag gcgccggaga aaatcgatgt tgtcggcgtc cacggcaagg
     7021 cgctgattcg ggtccttgct ctcgcgggcc ttttgggcat tgagcgtccg ctgctccaac
     7081 tcgctctgga ccgttcgtac gcgctcgggt gccagcgcct tcaccttgcc cagcggggct
     7141 ggatcgaggc tcaccggctt ggggatctcc gacagccgcg tgtactcgcg gccgccgccg
     7201 tcgcgctcga tacggagccc ttcggaccgc agggcgacct tccagctctc gccgaagccg
     7261 gaccggcgca ggagcgcttc ccgcccctcc aggctcacca gcccacgaac ggtaagacgg
     7321 ccatcctccc aggtgatcac tttgtccttg gcgaagcgga cgagccccct tccccccttc
     7381 tcccgccaga cgccttcgag ctttgacgga tccgaaatcc cgatctccac cccctggacc
     7441 ggctcccatt ccgccgcttc gagatcctcc cagggtatgc gccagaggcc gtgctgagcc
     7501 cgggaagggg cgacgtagag cccatcccat cccgaagcca tggaggctcg gcccgggatc
     7561 gggataggca gccacatccg ctcgaggacg gcgcgggcgg gatcgaagcg ctcgagagag
     7621 acctgcgagc ccgcggtgcc ggagtcccca tggacgagga agtacatacg gccatctcgc
     7681 ccttcgctga gggcgatgcc catgggtcgg gccgtcatct ccatgacttg cgaccgtata
     7741 ccttgttcgg ccaatgcctt ctggtagttg gccccctgct gccggcgggc ttcggcatcc
     7801 tcgacctcgt gaacgtctcc accgtcgacg acgatctccg tgaggaggcg gcccgaagag
     7861 ctgtactcat gaaactggtg ccggtaggca aatgccgccc ataggcggcc gtccgtggtg
     7921 tttcccagcc gtagcgctct ccgcacatcc agggccatcg ggtcgggctc cgcgagcttc
     7981 tccgcagtcg caccgaaggc atcccatcgc tgtccccgcc aggtcagcat cagcggagcc
     8041 ggtttc
//
LOCUS       DASPJI010000510         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_378442, whole genome shotgun sequence.
ACCESSION   DASPJI010000510 DASPJI010000000
VERSION     DASPJI010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378442"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2192
                     /locus_tag="VEL74_13835"
     CDS             <1..>2192
                     /locus_tag="VEL74_13835"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYG63657.1"
                     /translation="VRLAGAGDWQRGGQGGGSAHEGAEHGQPDPAREGDTLAYLLYTS
                     GTTGRPKAVMVSQANLAHTLRSSQQAFGFQPGDRVPCLAAFSFDIFLLELLGPLLAGG
                     TAVLYGLGVDLDLPRLASDLPSMTLLHAVPALMGQIVDELDRQGIRPAVRRAFVGGDA
                     VPAELLARMREAFPGARITVLYGPTEATIITTFHEVDGRESRPLIGRPLPGSTVELRD
                     RDGNLVPIGVPGEVWLGGPAVTRGYLHRPELTAEVFVPGTGGRFYRTGDLGRRLADGN
                     VEFLGRTDHQVKVRGIRIEPGEIEAVLAAHPEVRAAAVLVRRSGEGDARLVACVVPRT
                     GAAGDLPDELRQWLRVRLPEAMVPAGWAVLDALPLTPHGKVDRRALASAEAEEPVGGA
                     AWAAPRTPTEELLAGLWAGLLGRERVGLHDDFFDLGGHSLLATRLVTRMAGAFGVEIP
                     LRAVFEAPTVAGLARKVEEARAGSRKGQVPPIQPVSREGALPLSFAQERLWFLDRLEP
                     DRALYNVPFALRLRGPVEAVALERGLREIVRRHEALRTTFPDTGGEPRQEVASFRAFP
                     LPDVDLSALPEREREAEARRRLLEEGRRPFDLVLGPLLRVLLIRLGSDEALLLVNTHH
                     IVSDGWSMGVLTGELAALYASSPLPELPVQYADFAVWQRRWLSGEVLERQIAYWRKEL
                     AGAPPLLELPTDRPRPARQSGRGGDRPVALTPELGRAVRSLARQEGATP"
BASE COUNT          253 a          772 c          843 g          324 t
ORIGIN      
        1 gtccggctgg ccggcgcagg cgactggcag cggggggggc agggaggggg gagcgcacac
       61 gaaggagccg agcacggcca gccggacccg gcgcgcgagg gggataccct cgcctacctc
      121 ctttacacct ccggcaccac cggccggccg aaggccgtca tggtgagcca agccaacctc
      181 gcccacacgc tccgctcctc ccaacaggct ttcggcttcc agccgggcga ccgcgtgccc
      241 tgcctggcgg ccttctcctt cgacatcttc ctcctggagc tgctggggcc gctcctcgcc
      301 ggcggcaccg cggtcctcta cggcctggga gtggacctcg acctgccgcg tctggcctcc
      361 gatctcccct ccatgacgct gctgcacgcg gtcccggcgc tcatgggcca gatcgtggac
      421 gagttggatc gccaggggat ccgtccggcg gtgcgccgcg ccttcgtggg cggtgacgcg
      481 gtccccgccg agctgctggc ccgcatgcgt gaggccttcc ccggggcgcg gatcacggtg
      541 ctttacggtc ccaccgaggc gacgatcatc accaccttcc atgaggtgga cggccgcgag
      601 agtcgccctc tcatcggccg gccgctgccg ggctccaccg tggagctacg cgaccgcgac
      661 ggcaacctgg tgcccatcgg cgtgcccggc gaggtctggc tgggtggtcc ggcggtgaca
      721 cgcggctacc tgcaccggcc ggagctgacg gcggaggtct tcgtaccggg caccggcggg
      781 cgcttctacc ggaccggtga ccttgggcgc cgccttgccg acggtaacgt ggagttcctg
      841 ggccggaccg accatcaggt caaggtgcgg ggcatccgta tcgagcccgg ggagatcgag
      901 gcggtgctgg cggcccatcc ggaggtgcgc gcggcggcgg tcctggtgcg caggagcggc
      961 gagggcgatg cgcgcctcgt cgcctgtgtg gtgccgcgca ccggcgcggc cggcgatctc
     1021 ccggacgagt tgcggcaatg gctccgcgtc aggctgcccg aggccatggt gccggccggg
     1081 tgggcagtcc tcgacgctct gccgttgacg ccgcatggca aggtggaccg gcgggccctc
     1141 gcaagcgccg aggcggagga gccggtcgga ggagcagcct gggcggcgcc ccgcaccccc
     1201 accgaggagc tgctggcggg gctctgggcc gggctcctcg gccgggagcg ggtcggcctc
     1261 catgacgact tcttcgatct cggcgggcac tctctgttgg ccacccggct ggtgacacgg
     1321 atggcgggtg ccttcggcgt cgagatcccc ctgcgggcgg tgttcgaggc tccgaccgta
     1381 gcggggctgg cgaggaaggt ggaggaggcc cgcgccggtt cccggaaggg ccaggtgccg
     1441 ccgatccagc cggtttcccg tgaaggcgcc ctgccgctct ccttcgccca ggagcgcctc
     1501 tggttcctcg accgcctgga gcccgaccgg gcgctctaca acgtcccttt cgccttgcgc
     1561 ctgcgtggcc cggtcgaggc cgtggccctg gagcgcgggc tgcgggagat cgtgcggcgc
     1621 cacgaggcgt tgcgcaccac cttcccggac accggcgggg agccacggca ggaggtcgcg
     1681 tcgttccggg cgttcccgct gccggacgtc gacctctcgg cgctgccgga gagagagcgc
     1741 gaggcggagg cccgccgccg cctgttggag gaagggcgac gcccctttga cctggttctg
     1801 gggccgctgc tgcgggttct gctcatccgg ttgggatcgg acgaggcgct gctgctggtc
     1861 aatacccacc acatcgtcag cgacggctgg tccatggggg tgctgaccgg tgagctggcg
     1921 gctctgtatg ccagctcgcc cctgccggag ctgccggtgc agtacgccga cttcgcggtc
     1981 tggcagcggc ggtggctgtc gggcgaggtg ctggagcggc agatcgccta ctggcggaag
     2041 gagctggcgg gcgctccgcc gctgctggag ctgccgaccg accggccgcg ccccgcgcgg
     2101 cagagcggac gcggcggcga ccggccggtg gcgttgacgc cggagctggg gcgggcggtc
     2161 cgctcgctcg cccggcagga gggggcgacg cc
//
LOCUS       DASPJI010000511         4418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_544282, whole genome shotgun sequence.
ACCESSION   DASPJI010000511 DASPJI010000000
VERSION     DASPJI010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544282"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VEL74_13840"
     CDS             complement(<1..847)
                     /locus_tag="VEL74_13840"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYG63658.1"
                     /translation="MLERLLEIGKRVLAETDVDRVLTVALDGVVELCGAERGMILLFG
                     EDSEPLFEEARNLARQDIERPEFEVSRSILGKVRSEGTPFWSPNVLADPSFGSRRSVL
                     RLQILSVICLPIRHGGRIFGLVYLDNRSAMGVFTPETCALVASFAELISLAAHSALER
                     RSLRGRVEELSRELRERYQFDSILGQDPKILEVLRLVAQVAESDVTVLVLGESGTGKE
                     LVARALHYNSRRRERPFVAVNCGALPETLLDSELFGHVRGAFTGAVADSPGWFERAAG
                     GTIFLD"
     gene            1083..1772
                     /locus_tag="VEL74_13845"
     CDS             1083..1772
                     /locus_tag="VEL74_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63659.1"
                     /translation="MNIRKALQASAFGLTLIVLATGAAGAQCTTGLFYADLKANFATQ
                     FIPGVVDLISPLIASTAQGCAGWQEGVVRIRIPNNCTAAIVLAEYEGLPQGYTLNLGD
                     SPTNDAHGGDGGSTANDAELWVHQENMSVANGSGIASSPTLDNPLISQHLALTDSALK
                     LVVKHQYLSWGQPFGFLQTPSSQNLFAIPDPDPAVAQADKRAIYLGMNRVIQDNSRNG
                     CGLRRVLVKFQ"
     gene            1859..3592
                     /locus_tag="VEL74_13850"
     CDS             1859..3592
                     /locus_tag="VEL74_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63660.1"
                     /translation="MPARTLPIALLGILLGLSCCAGKVEETQPAETQAVPRAEAEVRS
                     GRVRLLVPAGEGSRPEYARLAAELDRAAGEMVRRVGGAPDESPLTVLIEGDHVAQARS
                     AGDIGEAVPGPRADLHLVWHPDDLPAYRFALARVLVARAGIGKQLPPWFERGAALWLS
                     RDWYGRTYSDWLPLLARARVLPEPEQLLLAAYAPWPRDASTLLWTPAAAAVVERLPGA
                     TVAEKLREIPMRERVAATLNALTKASNVGKGASPSPTRKPEMPSAFMKGVSLAMLNRV
                     EEGYHAPAVDRQLERLHSLGANAVSLMPFAGQAAVDRPEMRYLSRHPASETDIGLIHA
                     ARRARARGFLILWKPHLWVRGSWTGEIAMKSEADWVLWWKGYRRYVLHHALLARWAGA
                     DLFSVGVELSKTLGREREWRELIADVRLFFPGPVTYSANWYGDLESAPFWDSLDVIGV
                     DAYFPLSASPEAKRADLEAGARQIAQRLEATWRRHRKPVLLTEVGFPAHRGAWIEPHT
                     EGGEYSEADQALAYQALFKGLGRPSWLAGTFLWKAFSGEGGDRGSADFRFLGRQAEAA
                     VREYYSGRRLP"
     gene            4040..>4418
                     /locus_tag="VEL74_13855"
     CDS             4040..>4418
                     /locus_tag="VEL74_13855"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HYG63661.1"
                     /translation="MSDDLLETAVAAARAGSEVVKRYFRDAALQVDRKGQNDFVTQAD
                     RESETAVLGEIRRRFPDHRILAEEGGGAGVGQGSEYQWLIDPLDGTTNFMQGLPVYCV
                     SVACRRGDELVVGVIDDPEGGNVF"
BASE COUNT          694 a         1528 c         1506 g          690 t
ORIGIN      
        1 cgtcgaggaa gatcgtcccc ccggcggcgc gctcgaacca ccccgggctg tccgccaccg
       61 cgccggtgaa ggcgccgcga acgtggccga acagctccga gtcgaggagc gtctcgggca
      121 gggcaccgca gttgaccgcc acgaacggcc gctcgcgccg ccggctgttg tagtggaggg
      181 cgcgggcgac cagctccttg cccgtgccgc tctcgcccag caccaggacc gtgacgtccg
      241 actcggccac ctgggccacc aaccgcagca cctccaggat cttggggtcc tggccgagga
      301 tggaatcgaa ctggtagcgc tcccgcagct cccgggacag ctcttcgacc cggccgcgga
      361 ggctccgccg ctcgagggcg ctgtgggcag ctaaggagat gagctcggcg aagctcgcga
      421 ccagtgcgca ggtctcaggg gtaaagaccc ccatggcgct acggttgtcc aggtagacca
      481 ggccgaagat ccgcccgcca tgccggatgg gcaggcagat gacggagagg atttggaggc
      541 gcaggacgct ccgcctcgag ccgaaggacg ggtcggcgag cacgttgggg ctccagaacg
      601 gcgtgccctc ggagcgcact ttgccgagga tcgagcggct gacctcgaac tcgggccgct
      661 cgatgtcctg gcgggcgagg ttccgggcct cctcgaacag cggctcgctg tcctccccga
      721 agagcaggat catgccgcgc tcggcaccgc acagctcgac gacgccgtcg agcgccaccg
      781 tcagcacgcg atccacgtcg gtttcggcca gcacccgctt gccgatctcc agcaggcgtt
      841 ccagcacgtc atgtccccct ttgccggcaa ggataccgcc gttgcgggtg tgatcaccat
      901 acctatggtt ttcacacccc aaggccgggc gctccaatac cttcaacaga tgctgccgtc
      961 ccgctacaag actctataga tcaatgactt gcctctcttt cctgccacgc tgtcgaggtg
     1021 ggcaccgccc ttgccctttc aggggtcgac ggaaaaaccc atcaggaaaa ggagatatca
     1081 ccatgaacat tcggaaggcc ctgcaggcgt cggcgtttgg tctcaccctg atcgttctgg
     1141 cgaccggcgc ggccggggca cagtgcacga caggcctctt ctacgcggac ctcaaggcca
     1201 acttcgccac tcagttcatt ccaggggtgg tcgacctgat ctcgcccctg atcgccagca
     1261 ccgcgcaagg gtgcgccggc tggcaggagg gggtggtgcg gatccgcatc ccgaacaact
     1321 gcacggcggc cattgtcctg gccgagtacg aggggctgcc gcaggggtac accctgaacc
     1381 tgggggactc gccgaccaac gacgcccatg ggggtgacgg cggcagcacg gccaacgacg
     1441 ccgagctctg ggtgcaccag gaaaacatgt ccgtcgccaa cggttcgggc atcgccagct
     1501 cccctacgct cgacaacccg ctgatctcgc agcacctggc cctgaccgac agcgcgctga
     1561 agctggtggt gaagcaccag tacctgagct ggggccagcc gttcggcttt ctccagacgc
     1621 cgtcgagcca gaacctcttc gccatcccgg accccgatcc cgcggtcgcc caggcggaca
     1681 agcgcgcgat ctatctcggg atgaaccggg tgatccagga caactcccgg aacggctgcg
     1741 gcctgcgccg cgtgctcgtc aagttccagt aagacatccg cgcccctgcc cggcctccac
     1801 accgtccggc gcttctcccc tggggcgccg gcggtctttt tcttagtacg atccacccat
     1861 gccggcccgg accctgccca tcgccctcct cggaatcctc ctcggcctct cctgctgcgc
     1921 tggaaaggtg gaggagaccc agccagccga gacgcaggca gttccgaggg ccgaagccga
     1981 ggtccgaagc ggacgcgtgc ggctcctggt gccggcgggg gaggggagcc ggcccgagta
     2041 cgcgcggctg gccgcggagc tggaccgcgc cgctggcgag atggtgcggc gggtcggcgg
     2101 ggcgccggat gaatctcctc tgaccgtcct tatagaaggg gatcacgtcg cccaggcgcg
     2161 gagcgcaggg gacatcggcg aggcggtgcc cggcccgcgg gccgatctcc atctggtgtg
     2221 gcacccggac gacctgccgg cctaccggtt cgcactggcc cgggtgctcg tcgcccgggc
     2281 cgggatcggc aagcagttgc ccccctggtt cgagcggggc gcggccctgt ggctctcgcg
     2341 cgactggtac ggccggacct actccgactg gctgccgctc ctcgccaggg cccgggtgct
     2401 ccccgagccg gagcaactcc tcctcgccgc ctacgctcca tggccgcgcg acgcctcgac
     2461 gctcctctgg accccggccg ccgccgctgt ggtggagagg ctccccggcg ccacggtcgc
     2521 cgagaagctc cgcgagatcc ccatgcggga gcgggtggcg gcgacactga acgcactgac
     2581 caaagcctcc aatgtaggaa agggggcaag cccctcccct acaagaaagc ccgagatgcc
     2641 ctccgctttc atgaagggcg tctccctcgc catgctcaac cgagtggagg agggctacca
     2701 cgctcccgcg gtcgaccggc agctcgagcg gctccacagc ctgggggcga acgcggtctc
     2761 cctgatgccc ttcgccggac aggcggcggt cgaccggccg gagatgcggt acctcagccg
     2821 ccatccggcg agcgagaccg acatcggcct gatccacgcc gcccgccgtg cccgggcgcg
     2881 ggggttcctc atcctctgga agccgcacct ctgggttcgc gggtcgtgga cgggcgagat
     2941 cgcgatgaag agtgaggcgg actgggtgct ctggtggaaa gggtatcgcc gctatgttct
     3001 gcaccacgcc ctcctcgccc gctgggccgg ggccgacctc ttcagcgtcg gcgtcgagct
     3061 gtcgaagact ctgggccggg aacgcgagtg gcgcgagctg atcgccgacg tccgtctctt
     3121 cttccccggg ccggtcacct attcggccaa ctggtacgga gatctcgaga gcgcgccctt
     3181 ctgggactcc ctggacgtga tcggcgtcga cgcctacttt cccctctcgg cctcgccgga
     3241 ggccaaacgc gccgacctcg aggccggagc gcggcagatc gcccagcggc tggaagcgac
     3301 gtggcgccgg caccgcaagc cggtgctcct caccgaggtg ggtttccccg cccaccgcgg
     3361 cgcctggatc gagccgcaca ccgagggggg cgagtactcg gaagccgacc aggctcttgc
     3421 ctaccaggcg ctcttcaagg gactcggccg cccctcgtgg cttgccggta ccttcctctg
     3481 gaaagccttc agcggcgaag ggggcgaccg cggaagcgcc gatttccggt tcctggggcg
     3541 gcaggccgag gcggcggttc gggagtatta ttcgggcagg aggctcccat gagcgtaccc
     3601 aaaccaatcg ggcgtgccgc gaccgtgaca gccctgctct tcgtcgcggc ctgcttgttc
     3661 atcgacgccg gtacgttcca catgatgccc gggataggtc cttgcctgag cgggttggga
     3721 gtgttgctcc taccgctcgg cgtggggatc gctgccgctc ccatcgaggg gctcctctcg
     3781 ttggccctgg tcattcgggc tgtctatgta gcgttccggc ggcgccccgt gcgtatcgta
     3841 gaaaagcctg gaaacccatg agcgctccgc ggcggtttcc gaaggacatc gtggccgcga
     3901 tccagaaaac agccaggtgc tgggcatccg cgccggcacc gggccgcacc ggatttcatt
     3961 gttgagccgg ggagctgatg gataggatcg ctagcagtgt cacggggcgg gcacggacct
     4021 tgcggcataa taggccgtca tgagcgacga cttgctggag accgcggtgg ccgcggcgcg
     4081 cgcgggctcg gaggtggtga agcgctattt ccgtgacgcg gcgctgcagg tggaccgcaa
     4141 ggggcagaac gacttcgtca cgcaggccga ccgggagagc gagacggcgg tgctgggcga
     4201 gatccgccgg cgcttcccgg accatcgcat cctggccgag gaggggggcg gggccggcgt
     4261 gggccagggc agcgaatatc aatggctcat cgatccgctc gacgggacga ccaacttcat
     4321 gcaggggctt cccgtctatt gcgtctccgt ggcctgccgc cggggcgacg agctggtggt
     4381 gggggtgatc gacgaccccg aggggggcaa cgtcttca
//
LOCUS       DASPJI010000512         4433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_31579, whole genome shotgun sequence.
ACCESSION   DASPJI010000512 DASPJI010000000
VERSION     DASPJI010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31579"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1396..2157)
                     /locus_tag="VEL74_13860"
     CDS             complement(1396..2157)
                     /locus_tag="VEL74_13860"
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG63662.1"
                     /translation="MRCVEHQENLYGTDVHPEVAASLHELARVLRAQGDLAGARERLE
                     RSLAIKAEVLGTEADPSVAASLHQLAGILQAQGDLSGARERLERSLAIQAQVFGTEVH
                     PDVAASLHELARVLQAQGDLSGAREELERSLAIKAQVFGTEAHHDVAASLHELAGVLQ
                     AQGDLTGARERLEKVLEIEAQVYGTREHYSTAMTEERLGLLLLEQGEIKQGVELLVHA
                     YTVYQKQLGPDHPYTRQLAELFAQFAPESPEVSPP"
     gene            complement(2157..>4433)
                     /locus_tag="VEL74_13865"
     CDS             complement(2157..>4433)
                     /locus_tag="VEL74_13865"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYG63663.1"
                     /translation="PDRPLSIVATVAAPVDAGPFDHEEEMYLLWRALGEKKQEKRLLI
                     TDRGILDDLAGAVERHRPPVVHFTGHGRPGELLFEDEEALSDPVDVTELIRRFRETGP
                     LPRLIYLSACHGTTAEPAAAESHPGERMVDHAAVDRTAPSTAASLHRAGFPQVVAYFG
                     PVQDRQATRAASAFYAALGRGRTAWEALRQARRISAEPLRDAGRATHVYPLGWAQLAL
                     YHRGPDHATTLPPASGGATVDLPQEERKRIFERLDQAGGSQRVEHGIVGVQRLRFGFV
                     GRRRERAEAIRRWKKGQRLLVVQGLGGLGKTTLCAEVAPVLAETLRPGTRVLALDGRY
                     AGTQPDPVAALWQEVQAARSDQGWSQVLAGLQKEGLTAEALARALVTLAQIEEGLLVY
                     LDDAESLQARVGEGEVGRWRDQGFAQFWQYLVEAAEGGEAFGILASSRYQPEGTPSRA
                     VLPLPEMSRYEVVRLLSWMPTLGRMPLADRAWVAEKVEGHPRTVEYLEALARAKEEDI
                     VPPGGQYAGGRWREEILEPILPETEKKVDSDLLLGKLWESLPPAAQEHLGRCSVLTAP
                     VPWQAIQALALESDTDDRLFEAGLLSPFQPPFEKEVWWAPHRLVAEEALSRWSGEPKK
                     AHLLLAEWLEKRFEAESTDYWRTRAVEHFLAAGEADRAWPTARLLILSLRHAGRYQEA
                     LGWAERVLAAGPTGGALGKALTFKVQLQISAGLLEPEAEGTLLRALELVAREDKGFVF
                     AQLGALCNQQGKLRMPNT"
BASE COUNT          736 a         1578 c         1408 g          711 t
ORIGIN      
        1 cgagctgcag gaagctctcg gagaaagcgt tggaacggct ggacggacgg ggggtgttac
       61 cctcttcaga gttgcgcatg ggatatcgct cctttgcgtg acaggctcgg atgagggctc
      121 ctaacccctc atccgggccg cctggttgtg atgctgcaca ccggccggca ccctgccggc
      181 cgcttcgctt tgtggcgccg ggtccgcgcc gctcgccttc tctctctccc cgcgatcacc
      241 ccctgtccgg tcgtgaactt gtgcttccag gcggtactct atgacgggca agttgcgagg
      301 tcaaggggga aaatgaaaat cggccgatga ccggcaaagc tccgccgatc gaaggcaatg
      361 ccgtgcccgt caacggcaga ccgcgccctg tgacaggcac cgcgcggccg acgacaggca
      421 aggtgcggcc gacgacaggc cgcaccctgc cgatgaccgg ccgcgcgatg ccgacggccg
      481 gccgagccgt gccggcgaca ggccacgccg tgccccggac gggcgccgcg tggccctccg
      541 ttggctggga tctcccgaga atcggcagag cggggccggc atccgggaga tgagcgcccc
      601 gcttcggcag tgagctgcac agcaacggca gcccttcccc caggaaacgc aaccctcccc
      661 ccaggaacgt ccaccgtctt tccgggtaca gccttcctac cccggggatg aaccccatcc
      721 ccgtgggtac ggagtccgta cccacgggta cgaggccatc cccgcgggta cggccgtcct
      781 acccgcaggg atgacgatcc tacccggggt acggcctttc tacccgcggg gatgaccacg
      841 atctcccggg gtacgaagct cttttcccgg gggatggcaa tcgtctcccc agggatggcg
      901 atcgctccag cgggtacggc gacggttctc gcggggatgt cgatcgtcct cacgagatcg
      961 cgaccctttc caggggggat gacgaacgtt tcacaggaga tgacgatcgt ttcgagaggg
     1021 acagcgacag ccagccccag gttcccgagg acgcctggga agggggcttc ttccccccct
     1081 gggcgggtgc gtggtaggga gtgggggagg atcgaggagg ggggttttcc accacctgcc
     1141 aagacccgga cgccggatct cggcaccggc gttcctcagc cgcgaagcgg cgcaggccta
     1201 gagcctggca tttcaatgcc aggtacgccc ccccaaccct cccagccctg aaaggccgca
     1261 ggggccgccc ccggaccgcg agctgcctgc gtcccttcgg gactttggaa tcaatgggtt
     1321 gcgtctacct ggcattgaaa tgccaggctc tacgccggcg ccgcttcgcg gctggatggg
     1381 cccccccgag gtctctcaag gcggagagac ttcaggagat tcgggggcga actgggcaaa
     1441 caactcggcc agttggcgcg tgtaggggtg atcgggaccg agttgcttct ggtagacggt
     1501 gtaggcgtgg accagcagtt ccaccccctg cttaatctcg ccttgctcaa gcaggagtaa
     1561 acccagtctc tcctcggtca tagcggtcga gtagtgctcg cgggtcccat agacttgggc
     1621 ctcgatctca agaaccttct ccagcctctc gcgggctccc gtcagatctc cctgcgcttg
     1681 gagaaccccc gccaactcgt gaagcgacgc tgccacgtca tggtgagcct ccgtcccgaa
     1741 cacctgcgcc ttgatcgcca gagatctctc cagttcctcc cgcgctcccg acagatcgcc
     1801 ctgcgcttgg agaacccgcg ccaactcgtg cagcgacgcc gccacgtcag ggtgaacctc
     1861 agtcccgaac acctgcgcct ggatcgccag agacctctcc agcctctccc gcgctcccga
     1921 cagatctccc tgcgcctgga gaatccccgc caactggtgc agcgacgccg ccacggatgg
     1981 atcagcctcc gtccctaaaa cctctgcctt gatcgccagt gacctctcca gcctctcccg
     2041 agctccggcc aaatctccct gcgctcggag aacccgcgcc aactcgtgca gcgacgccgc
     2101 tacctctggg tgaacatccg tgccatagag gttttcctgg tgctccacac agcgcatcag
     2161 gtattgggca ttcgaagctt tccctgttgg ttgcatagag cgcccaactg agcgaagacg
     2221 aagcctttgt cctccctcgc taccagctct aaggcgcgga gcaacgtccc ttctgcttcg
     2281 ggctctaaca acccggcaga aatctgtaac tggaccttga aggtcagcgc cttccccaaa
     2341 gcccctccgg tcgggccagc cgcgagaacc cgctccgccc aaccaagcgc ttcctggtag
     2401 cggccggcgt gacggaggct caggatcagc aggcgggccg tgggccacgc gcggtccgcc
     2461 tcccccgccg cgaggaagtg ctcaaccgcc cgagtccgcc aatagtcggt gctctccgct
     2521 tcgaatctct tctcgagcca ctcggccaac aagagatgag ccttcttcgg ctcgccactc
     2581 catcgactca gagcttcttc ggcgaccaac cggtgcggtg cccaccagac ctctttttcg
     2641 aaaggcggct ggaaagggct caacagccca gcctcaaaaa gccgatcatc ggtgtcactc
     2701 tcgagagcca gcgcctgaat cgcctgccag ggaacaggag ccgtcagcac cgagcaccgc
     2761 ccgaggtgtt cctgggcagc cggcgggagc gactcccaga gcttgccgag aagaagatcg
     2821 ctgtcgacct tcttctcagt ctcgggcagg ataggctcca ggatctcctc ccgccagcgc
     2881 ccccctgcgt actgcccgcc gggaggaacg atgtcctcct ccttcgcccg cgccagcgcc
     2941 tccaggtact ccaccgtcct ggggtgaccc tccaccttct ctgccaccca ggcacggtcg
     3001 gctaaaggca tccttcccag cgtgggcatc cacgaaagaa ggcggacgac ctcgtagcgg
     3061 ctcatctccg ggagaggcaa gacggctctg gagggcgtcc cctcgggctg gtagcgcgac
     3121 gaagccagga tgccaaaggc ttcacccccc tccgctgcct caaccaggta ctgccagaat
     3181 tgggcaaacc cctgatcgcg ccagcgcccc acctcgccct cccccacccg ggcctgaagg
     3241 ctttcggcgt catccagata gacgagcagg ccctcttcga tctgcgcgag ggtaaccagc
     3301 gcccgtgcca atgcctcggc ggtgaggcct tccttctgaa gaccggccag cacctggctc
     3361 catccctgat ccgagcgcgc cgcctggacc tcctgccaga gggcggcgac cggatccggc
     3421 tgggtccctg cgtagcggcc atcgagggcg aggacacggg tgccgggcct caacgtctcg
     3481 gccaggaccg gagccacttc ggcgcagagg gtggtcttcc ccaaaccacc cagaccctga
     3541 accaccagca ggcgctgccc cttcttccac ctccggatcg cctccgcccg ctcgcgacgc
     3601 cggcccacga agccgaagcg gagacgctgg acgccaacga tcccgtgctc gacccgctgg
     3661 ctgccgccgg cttggtccag acgctcgaag atccgcttcc tctcttcctg gggcaggtct
     3721 acggtggcgc cgcccgaggc cgggggcaag gtggtggcat ggtctgggcc tcggtgatag
     3781 agggcgagct gcgcccaacc cagcgggtag acatgggtgg cgcgcccggc gtcccgaagc
     3841 ggctcggcgg agatccggcg cgcctggcga agggcttccc aggcggtgcg cccgcggccc
     3901 agggcggcgt agaaggcaga ggcggcgcgg gtggcctgac ggtcctggac cgggccgaag
     3961 taggcgacca cctgggggaa gccagcgcgg tggagggagg cggcggtgga gggggcggtc
     4021 cggtccactg cggcgtgatc caccatccgc tcgccgggat gggactccgc ggcggccggc
     4081 tccgccgtgg tgccgtggca ggcggagaga tagatgagcc gcggcagggg tccggtctcc
     4141 cggaagcgtc gaatcagctc ggtcacgtcc acgggatcgg agagggcctc ctcgtcctcg
     4201 aagagcagct cgcccgggcg gccgtggccg gtgaagtgga cgaccggcgg cctgtggcgc
     4261 tccacggccc cggcgaggtc gtccagtatc ccccggtcgg tgatgagcag ccgcttctcc
     4321 tgcttcttct ccccgagcgc ccgccagagg aggtacatct cctcctcgtg atcgaacggc
     4381 ccggcgtcca ccggcgccgc caccgtggcg acgatggaga gcggccggtc cgg
//
LOCUS       DASPJI010000513        11061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_841887, whole genome shotgun sequence.
ACCESSION   DASPJI010000513 DASPJI010000000
VERSION     DASPJI010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11061)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11061
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841887"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VEL74_13870"
     CDS             complement(<1..298)
                     /locus_tag="VEL74_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63664.1"
                     /translation="MTSTNILDVLRALVTLPADLFWPGVAWLLDHLRQLGATGAGVLV
                     ALLTATLAGLALVQQRHLNQALAANPARRLGFGPFLRMELWHSPFAIPLWPFRLL"
     gene            complement(302..4201)
                     /locus_tag="VEL74_13875"
     CDS             complement(302..4201)
                     /locus_tag="VEL74_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63665.1"
                     /translation="MSLVFPAPAIVVGVGRFGLAALERLGEDWMGLRLSGADVSLDNL
                     RLLAVLPDGAEDGWRRHEHDAVHIAGYLGDSDLPSLAVDLAILRSLGLVRFRDGSYQL
                     ALPRDTGAVEIEERDEDGVRKRLVRRRYFEWVKLSPDPVVAAERLRHLAQRLQEVDLF
                     VTPVLNRVKQGHSPWALLACLDRCRAYLDGRDPAPWGWLDTRERPAAGEPRLPQRIEV
                     SPNEAGEPPGPGEEREPSPAEIVVPFPFVRLPSDVGSPLDPVDLLDRDWANTGWASSG
                     GRTEGEVFELEPLSPPQLGLFDHDGREGSGGEGMDGSPQETAAALLDRRLRELGDHLH
                     RGLVRLWVDLQRERVAEPNINVLEKTRQRDELSEALQQTLEVLGEILVRPLTDNGAGV
                     QAVPDRSPSAAETELPTLPSDFLAGLEVGTREKDSSPLRLLEDRLTDLGLGDPAQWRR
                     WRRPLLETEHLTVPLETAEPGCAEHKGLRNLRLKLNQQVRQLFDFTFLTRYRNRPTRR
                     PPRLTVFVVGDMSEAFTREAMRPVLREIHAELLRAFTPIFESYREGFDRCLCVTPILW
                     MPHPADPFQGAGLELSRCEEAAIIDAVHGIRRWVECVLPPGRRCVHQIFVNSRVSDTA
                     ALSLADALRQTRDFIAFQTRNDLSADSWLRQTSTAAGGGDLFSSFSCYEIDFPALRCR
                     EYLANRLARECLAQLKEGEPTDVEPPEPFEPKDLDELADKPRKVLGEVTRDAAQNLAS
                     EVRARLDLSEATSYREVLQAFDERFGESLKAKVWARWSELTGRQGRVDDLVDELRLKT
                     SRLMGKTVTQVRDYSDALVQENAGAGGLTAALASFQGLKSTTRDSFQKREARRREKEA
                     LCERHGVPDLRPLKAVREGIEAAAARKPDLDPVRFGLALWAALSLVLGAPLAHGAAYL
                     LDLHLRPNLIELLLGPGGAIVGGLALWLPVRALLRWHMRRRLALLEAAVERLADEVHG
                     LLWGAGQPPERETRVSVRSFLESRLELTAAVAARGFALSVLERAVADARLGHRLARSV
                     DVQAEILMRQAEDLGVRVTLGAAGGRRESLRGLFERRTGDATDRLIDAEKLAGYYARR
                     VGQSLYRQVPGFIEAAGGFGPWREEACLADGGRLLQHGRDMFEDLVSEAVSDQHFFAD
                     EVGERLMRFIARNYSNLGFGAGFKGYEGLDPDNVLVLADATLVIHEGLQALFRAARQR
                     YGRQFPITDTMEVHLARTRPNAAYMLSLVQGVRVHSLRNLRRFESFHNRVALPDDRMF
                     PLSHEPRALGPAMNPLTGFEAVGADLAAAVLQRNGKG"
     gene            complement(4205..8059)
                     /locus_tag="VEL74_13880"
     CDS             complement(4205..8059)
                     /locus_tag="VEL74_13880"
                     /inference="COORDINATES: protein motif:HMM:NF025181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HYG63666.1"
                     /translation="MPRHHKVVIEEMLTKPSAVCPTLFVGLGGCGCRMVTRVAEHLRR
                     RPDYEERYRPLVKFALVDTNVNDLESFREKADATFLISDFEKEEYADLASGKLFLEAD
                     PYFTQWVPQNYRFRAGDTAGAGQIRIESRLGVFYQMKHKDFVPKFRKLLEDLKSHEHG
                     HRRLDSSEIRIVICYSVAGGTGSGSHLPIAYMLRDQASELGKPMVVGVAVLPAVFEDK
                     TGINKDGTFANGYAALKETEYLMRLGAPDSRFFPEEGLEFHYDPSNLSKRLVRQRPFE
                     FLYVIDKPQSFTVPDPVEAAADGLYLQFFSPLFGFQASDYDNYTQHQRFLVPHDFEAK
                     GIIGFTSFYGSYGTAVLLVPVDGLVEYCAQAAALSLMRASFLRAIPGEPVYDVLRNAP
                     EPFYEVTLSDGKNEKPVHVSDFQKKEADQQERLQDRLYMKRVRLLARYELDEGEARRF
                     LTLFRHGHRVGEVPTGGGGWELDAKRVKADQEQLARNGMRFSIAGIVLPAVSADRGSS
                     EGVQEMAGLLAQARQAMEAHIQQNPPTSTGASRVFELKNRATTWLDELKSIGMRILKN
                     GYKLGTVEYPGMDALVELDFLNEQAGAVDLAAKRYAILSILERVRWDQKLSDERITEF
                     SADDHADGDKIVAKDVPTLLGKLQSQATDWAMQEVTRLFLDRLSDFQKNLQKFVRLQR
                     DLERTFDQLEGEQLNHLRRLRESGGRSSSANQYVLDAEALQIEDGRRMWDFYFEDRIA
                     DLPELSLENRDIQVHLSNTITEMSIRRGGGTSGDLDKLFTTLRQHVEGFLFSRINGDP
                     HSPDRERRDGLTLSDALELEVVYRAIYRSNTPEVDKEGHKVIRELVSRYRHLPPEEQI
                     DLKEDRYKDYLRDKIKRLVKEKASLLCVYEEGRDQHGGVRPDNVFVAAIDEDFKSGTI
                     ETAIRSADPNLTWLTTGWNNPKEIVFYRAVLNVPLYVFGRMKEMKSEYERFRNLAKRP
                     KVLHIDHNWENSLPDLDPDSAQENHRQELLRSHVINFASLLTTPHPGGRASFIVRHGG
                     KYCLVDPVQEERPGNGAPVEDELTVLGETLAESIQRLPEVLESEMVKYLPYQQMLRAV
                     RDGLAPEVLSQVVQLPFQWRKNHDELRNKYGSNRTPTQQEKLKDYSDAYQRLREALDE
                     LYDKLSNFSVEQRTLGEDIGFNSANLSQEEAQRSLDQSLKILATFTEVWSALEDPDRP
                     GAVSRSFQDLFRPLDEKKLTVLLERLRNNPIRPQKTGHEAAGRDHDGQPSPEASAIPP
                     MSPAVTAPAPPAATLDDAPAGS"
     gene            complement(8108..10882)
                     /locus_tag="VEL74_13885"
     CDS             complement(8108..10882)
                     /locus_tag="VEL74_13885"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HYG63667.1"
                     /translation="MNVPNQAMLRLALLLGLSLLAFEAAAQSEISSSGARAAQFVFIV
                     DDSGSMKETDPDRLAVFAVQALVGMLDDRDEVSLVRLNGPRDGAPVPPIEPLRQNRKK
                     IEDLLDLDGGLAHYEAKNTPCGSALGAVHRILQEAQRPGVSQVVMLLTDGECTPKDEE
                     PSVDGFLRGLRSRDEGLFQFYLLGFRGKRSSPGLADLAERTGGQAVTANPGDPASILQ
                     TFAAALSRSQGYEPYLLSPQSRHLAAHRGAERVRLLAIAPGSGADLSFSVRNPRGGSP
                     QTAGQPRTGTHRYGNGRAFRYAALDYRPDAEPVEVDVQGAGETWKVVALPEYRLTVRL
                     AVLQGTCEQPGPATGGGAETGSTVCAVAELVNDAGEAVGGEVTGGDLTASVRVRRPDA
                     PGTPPVEVAANQLPGGQARFGLLRSNLTRGDYELQPVVTLRLSSGDDVTLRGAPVSLV
                     VSSLTIEPRPGNFDFGRLRPGGRAARRLTLGGSFPQTPGHAELDSRAELPSCITVELG
                     KEPEGKAQPILVNNPYTLTLRAHPYCGPRPFSRSFNSAVRLVFGESAGGRRIPAVVLP
                     VRFHVDYQISVPPELTVAVRGGQATDLAVPVRGNFEQEVPLRAVVAGPADAEAWPEDP
                     ENLRLGFAGERRKDLLEDGEGGALLVHDFIASPGGPPLRLRARPDRCCAGGTYRTRLG
                     LAPAGSQPLPPGARAPEPILVPVRIDVEPAGFWACYGPRVLLGLALLLLLLLVLYIAN
                     MIRNSAFLRADTLAGKLKPLVWTEYGDAVEQKSSKNDVQLLVRRGMPLAGRALAWLRA
                     NPLRFGLPGGRYRETVELLLQPNRDVARSQVSLLAEADLQSRAERQPESYVGRLFATA
                     SGGTTFVAVPDPGGRVSRLVWQDGFAPAAPEGEVPVPKAVKLRKARLLRPLEAWESHE
                     EGMPAGWQVG"
     gene            complement(10879..>11061)
                     /locus_tag="VEL74_13890"
     CDS             complement(10879..>11061)
                     /locus_tag="VEL74_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63668.1"
                     /translation="GSQRALMLLAVNDREPNVPLLLCLSNILDSDPDLGFQSNDFSKF
                     ELNPTPPVSDCRGGDS"
BASE COUNT         1687 a         3728 c         3958 g         1688 t
ORIGIN      
        1 ccagcagccg gaacggccac agggggatgg cgaagggcga gtgccagagc tccatgcgga
       61 ggaacgggcc gaagccgagc cgccgcgccg gattcgccgc gagggcctgg ttgaggtgcc
      121 gctgctgcac cagggcgagg ccggcgaggg tggcggtcaa cagcgccacc aacaccccgg
      181 caccggtggc gccgagctgc cggaggtggt cgaggagcca ggccactccc ggccagaaga
      241 ggtcggccgg aagggtgacc agagcccgca ggacgtcgag gatgttggtg ctggtcatgg
      301 ctcagccctt tccgttgcgc tgcagaacgg cggcggcgag gtcggcgccc accgcctcga
      361 agccggtgag ggggttcatc gccgggccga gcgcccgcgg ctcgtgggac agcgggaaca
      421 tccggtcgtc cggcagcgcc acccggttgt ggaacgactc gaaccgccgc aggttgcgca
      481 ggctgtggac gcggaccccc tggaccaggg agagcatgta cgccgcgttg ggccgcgtgc
      541 gggcgaggtg gacctccatg gtgtcggtga tggggaactg gcggccgtac cgctgccgcg
      601 cggcgcggaa cagggcctgg agcccctcgt gaatgaccag tgtggcgtcg gcgaggacca
      661 ggacattgtc cgggtcgagc ccctcgtacc ccttgaagcc ggcgccgaag cccaggttcg
      721 agtagttccg ggcgatgaac cgcatgagcc gctcccccac ctcgtcggcg aagaagtgct
      781 ggtccgagac cgcctcgctc accaggtcct cgaacatgtc ccggccgtgc tggaggaggc
      841 gcccgccgtc cgccaggcag gcctcctcgc gccaggggcc gaagcccccc gcggcctcga
      901 tgaagccggg gacctgccgg tacaggctct gccccacccg ccgcgcgtaa tagccggcga
      961 gcttctccgc gtcgatgaga cggtccgtgg cgtctccagt ccgccgctcg aagaggccgc
     1021 gcaggctctc gcgcctgccg ccggccgccc ccagggtgac gcgcaccccc aggtcttcgg
     1081 cctggcgcat gaggatctcc gcctgaacgt cgacgctgcg cgccagccgg tgccccaggc
     1141 gggcgtcggc gacggcgcgc tccagcaccg acagggcgaa gccgcgggcg gcgacggcgg
     1201 cggtcagctc cagccggctc tccaggaacg agcggaccga cacccgggtc tcgcgctcgg
     1261 gcggctggcc ggctccccag aggaggccgt gcacctcgtc cgccaaccgc tccacggccg
     1321 cctccagaag ggccagccgg cggcgcatgt gccagcgcag cagcgcccgc accggcagcc
     1381 agagggcgag cccgcccacg atggcgccgc cgggcccgag gagcagctcg atgaggttgg
     1441 gccgcaggtg gaggtccagc aggtaggccg ccccgtgcgc caggggggcc cccaggacga
     1501 ggctcagcgc cgcccagagc gccaggccga agcgcaccgg gtcgaggtcg ggcttgcgcg
     1561 cggcggcggc ctcgatgccc tcgcggacgg ctttcagcgg cctcagatcg ggcacgccgt
     1621 gccgctcgca gagggcctcc ttctcccgcc gccgcgcctc gcgcttctgg aacgagtcgc
     1681 gggtggtgct cttgaggccc tggaagctcg ccagcgccgc ggtgaggccg ccggcgccgg
     1741 cgttctcctg caccagggcg tcgctgtagt cgcgcacctg cgtcaccgtc ttgcccatca
     1801 ggcgggaggt cttgaggcgc agctcgtcga cgaggtcgtc cacccgcccc tggcggccgg
     1861 tgagctcgct ccagcgcgcc cagaccttgg ccttgagcga ctccccgaag cgctcgtcga
     1921 aggcctggag gacctcgcgg taggaggtgg cctcgctcag gtccaggcgg gcccgaactt
     1981 cagaggcgag gttctgggcc gcatcgcgcg tcacctcgcc caggaccttg cgcggcttgt
     2041 cggccagctc gtcgaggtcc ttgggctcga acggctcggg aggctcgacg tcggtgggct
     2101 ccccctcctt gagctgggcg aggcactccc gcgccaggcg gttggccagg tactcgcggc
     2161 agcgcagggc cgggaagtcg atctcgtagc aggagaacga cgagaacagg tcgccgccgc
     2221 ccgcggccgt ggaggtctgc cgcagccagg agtcggcgct caggtcgttg cgggtctgga
     2281 aggcgatgaa gtcccgcgtc tggcgcaggg cgtcggccag gctgagggcc gcggtgtcgc
     2341 tcacccggct gttgacgaag atctgatgga cgcagcgccg gcccggaggc agcacgcact
     2401 ccacccagcg gcggatgccg tgcacggcgt cgatgatggc cgcctcttcg cagcggctga
     2461 gctccagccc ggccccctgg aagggatcgg cgggatgggg catccagagg atgggcgtga
     2521 cacagaggca gcggtcgaac ccttcgcggt aggactcgaa gatgggggtg aaggcgcgca
     2581 gcagctcggc atggatctcg cgcagcaccg ggcgcatggc ctcgcgggtg aaggcctcgc
     2641 tcatgtcgcc caccacgaag acggtgaggc gcggcggccg ccgggtgggc cggttgcggt
     2701 agcgggtgag gaaggtgaag tcgaagagct gccgcacctg ttggttgagc ttcagtcgca
     2761 ggttgcgcag ccccttgtgc tcggcgcacc cgggctccgc cgtctcgagg gggacggtga
     2821 ggtgctcggt ctccaggagc ggccggcgcc agcggcgcca ctgcgccgga tcgccgagcc
     2881 ccaggtcggt caggcggtcc tccagcagcc ggaggggcga ggagtccttt tcgcgcgtcc
     2941 ccacctccag gccggcgagg aagtcgctgg gcagggtcgg cagctccgtc tccgccgccg
     3001 agggggaacg gtcagggacc gcctgcacgc ccgctccgtt gtcggtgagg ggccgcacca
     3061 ggatctcccc cagcacctcg agcgtctgtt gcagcgcctc gctcagctcg tcgcgctggc
     3121 gtgtcttctc cagcacgttg atgttcggct ccgccacccg ctcgcgctgc aggtccaccc
     3181 agagccgcac caggccgcgg tggaggtggt cgcccagctc ccgcagacgc cggtccagga
     3241 gggcggcggc ggtctcctgc gggctgccgt ccatcccttc gccaccggag ccctcccggc
     3301 cgtcgtgatc gaagaggccg agctgcggcg gtgacagcgg ctccagctcg aacacctcgc
     3361 cctccgtccg gccaccgctg ctggcccagc cggtgttggc ccagtcgcgg tccaggaggt
     3421 cgacggggtc gagcggagag cccacgtccg agggcagacg gacaaagggg aacggcacca
     3481 cgatctccgc cggcgacggc tcccgctcct ctcccgggcc ggggggctcc cccgcctcgt
     3541 tcgggctgac ctcgatccgc tgcggcaggc gcggctcgcc ggccgccggc cgttcgcgcg
     3601 tatcgagcca gccccagggg gccgggtcgc ggccgtcaag ataggcacgg cagcggtcga
     3661 ggcaagccag cagagcccac gggctgtgcc cctgcttgac ccggttgagc acgggggtga
     3721 cgaagagatc cacctcctgg agccgctggg ccaggtggcg cagccgctcg gcagccacca
     3781 ccgggtccgg gctgagcttg acccattcga agtagcggcg gcgcaccaga cgcttgcgga
     3841 cgccgtcctc gtcacgctcc tcgatctcga cggcgccggt gtcccggggg agggcgagtt
     3901 ggtagctgcc gtcgcggaag cgcaccagac cgagggagcg caggatcgcc aggtccaccg
     3961 ccagcgacgg caggtcgctg tcccccagat agccggcgat atgcactgcg tcgtgctcat
     4021 gacgccgcca gccgtcctcg gcgccgtccg gcaggacggc gaggagccgc aggttgtcca
     4081 gcgaaacgtc ggcgccggac aaccgcagtc ccatccagtc ctcgcccaga cgctccaggg
     4141 cggcgagccc gaagcgccct acgccgacga cgatggccgg agccgggaag acgaggctca
     4201 aggctcagct cccggccggc gcgtcatcca gagtggcagc cggcggcgcc ggggcggtga
     4261 ccgcgggcga catcggtggg atggccgacg cctccggcga cggctgcccg tcgtgatccc
     4321 tgccggcggc ctcgtgcccg gtcttctgcg gccgaatggg gttgttgcgc agccgttcca
     4381 ggagtaccgt gagcttcttc tcatccaagg gccggaagag gtcctggaag ctcctggaga
     4441 ccgcaccggg acggtcgggg tcctccaggg cgctccacac ctcggtgaag gtggccagga
     4501 tcttgaggct ctggtcgagg ctgcgctggg cctcctcctg actcagattg gccgagttga
     4561 agccgatgtc ctcccccagg gtccgctgct cgacgctgaa attgctcagc ttgtcgtaca
     4621 gctcgtccag cgcttcgcgc aggcgctgat aggcatcgga gtagtccttg agcttctcct
     4681 gttgggtggg agtgcggttg gacccgtact tgttgcgcag ctcgtcgtga ttcttgcgcc
     4741 actggaacgg aagctgtacg acctgtgaca ggacctcggg cgcgagcccg tcgcgcaccg
     4801 cacgcagcat ctgctggtag ggcaggtact tcaccatctc cgactccagc acctcgggca
     4861 gccgctgaat ggactcggcc agcgtctcgc ccaggaccgt cagttcatcc tcgaccggag
     4921 cgccgttgcc cggccgctcc tcttgcaccg ggtcgacgag gcagtacttg cccccgtgcc
     4981 gcacgatgaa gctcgcccgg ccgccggggt gaggcgtagt gagcaacgac gcgaagttga
     5041 tgacgtggct gcgcagaagc tcctggcggt ggttctcctg ggcgctgtcg gggtcgaggt
     5101 cggggagcga attttcccag ttgtgatcga tgtgcagaac cttcggccgc ttcgccaggt
     5161 tccggaaccg ctcatattcc gacttcatct ccttcatgcg gccgaagacg tagagcggca
     5221 cgttgagcac ggcgcgatag aagacgatct ccttggggtt gttccagccg gtggtcagcc
     5281 aggtgagatt ggggtcggcg ctgcggatgg cggtctcgat ggtgccgctc ttgaagtcct
     5341 cgtcgatggc ggcgacgaag acgttgtcgg gccgcacgcc gccgtgctgg tcgcgcccct
     5401 cttcataaac gcagagcagt gacgccttct ccttgaccag gcgcttgatc ttgtcgcgca
     5461 ggtagtcctt gtagcggtcc tccttgagat cgatctgctc ctcgggcggc aggtgccgat
     5521 agcgggagac gagctcgcgg atcaccttgt gaccctcctt gtccacctcc ggggtgttgc
     5581 tgcggtagat ggcgcggtag accacctcta gctccagggc atcggacagg gtgaggccgt
     5641 cgcgccgctc ccggtcgggc gagtggggat cgccgttgat acgggagaag aggaagccct
     5701 cgacgtgctg gcgcagtgtc gtgaacagct tgtcgaggtc accgctcgtt ccacctcccc
     5761 gccggatgct catctctgtg atcgtgttgc tcaggtgtac ttggatgtcc cgattctcca
     5821 gagataactc gggaaggtcc gcaatccgat cctcgaagta gaagtcccac atccgacggc
     5881 cgtcctcgat ctgcagcgcc tcggcgtcga ggacgtactg gttggccgac gagcttcggc
     5941 cgccgctctc ccgcagtcgc cgcaggtgat tgagctgctc gccttcgagc tgatcgaaag
     6001 tccgctcgag atcccgctgc agcctgacga acttctggag attcttctgg aagtcggaca
     6061 ggcgatcaag aaaaaggcgg gtgacctcct gcatggccca gtcggtggcc tgactctgca
     6121 acttgcctag taaagtcggc acatccttgg caacaatctt gtcgccgtcg gcatgatcgt
     6181 cggcgctgaa ctcggtgatc cgctcgtcgg atagcttctg gtcccagcgg acccgctcca
     6241 ggatggagag gatggcatag cgcttggcgg cgaggtccac cgcccccgcc tgctcgttca
     6301 ggaaatccag ctccaccagg gcatccatgc cgggatactc gacggtaccc agcttgtagc
     6361 cgttcttgag gattcgcatg ccgatactct tgagttcgtc gagccaggtg gtggcccggt
     6421 tcttgagctc gaagacgcgg ctcgcacccg tagaggttgg agggttctgc tggatgtgag
     6481 cttccatggc ctggcgtgcc tgagccagaa ggccggccat ctcctgtacc ccttcgcttg
     6541 agccgcggtc cgccgacacc gccggcagca cgatgccggc aatggagaag cgcatgccat
     6601 tgcgggcgag ctgctcctgg tccgccttga cgcgcttggc atcgagctcc cagccgccgc
     6661 cgccggtggg gacctccccc acccggtggc cgtgccggaa gagagtgagg aagcgccgcg
     6721 cctccccttc atccaactcg tagcgggcaa gaagccgcac ccgcttcatg tacagccggt
     6781 cctgcagccg ttcctgctga tcggcctcct tcttctgaaa gtccgagacg tgaaccggct
     6841 tctcgttctt gccgtcgctc agggtgacct cgtagaacgg ctcgggggcg tttcgcagca
     6901 cgtcgtagac cggctcgccg gggatggcgc gcaggaagct ggcacgcatc agcgacaggg
     6961 ccgccgcctg cgcgcagtac tccaccaggc cgtccaccgg caccagcagg acggcggtgc
     7021 cgtaggagcc gtagaacgag gtgaagccga tgatcccctt ggcctcgaag tcgtggggca
     7081 ccaggaagcg ctggtgctgg gtgtagttgt cgtagtcgga ggcctggaag ccgaagagcg
     7141 gggagaagaa ctgcagatag agaccgtccg ccgccgcctc taccgggtcc ggcacggtga
     7201 acgactgagg cttgtcgatg acgtagagga actcgaaagg ccgctggcgc accagccgct
     7261 tcgaaaggtt ggaaggatcg tagtggaact cgagcccctc ctcggggaag aagcgggaat
     7321 cgggagcgcc gagccgcatc aggtactcgg tctccttgag cgccgcgtag ccgttggcga
     7381 aggtgccgtc cttgttgatg ccggtcttgt cttcgaagac ggcggggagc acggcgacgc
     7441 cgaccaccat gggcttaccc agctcgctcg cctgatcgcg cagcatgtag gcgatgggca
     7501 ggtgcgagcc cgagccggtg ccaccggcga ccgaatagca gatgacgatg cggatctcgg
     7561 agctgtccag gcgccggtgg ccatgctcat gggacttgag gtcctccagc agcttcctga
     7621 acttcggcac gaaatccttg tgcttcatct gatagaagac gccgagacgg gactcgatgc
     7681 ggatctgtcc ggcgcccgcg gtgtccccgg cgcggaagcg atagttctgc ggcacccact
     7741 gggtgaagta aggatcggcc tccagaaaga gcttgcccga ggcgaggtcg gcgtactcct
     7801 ccttctcgaa atcgctgatc aggaaggtgg cgtccgcctt ctcccggaag ctctcgagat
     7861 cgttcacgtt ggtatcgacc agcgcgaact tgaccagagg cctgtaccgc tcctcgtagt
     7921 cggggcggcg gcgcaggtgc tcggcgacgc gggtgaccat gcggcagccg cagccaccca
     7981 ggccgacgaa cagtgtcggg cagaccgcgc tcggcttggt cagcatctcc tcgatgacga
     8041 ccttgtggtg tctcggcatc tcttcatttc ctcccgcgga acagagctcc gcgaatccat
     8101 tgcgcggcta gccgacctgc cagccggcgg gcattccttc ttcgtgggac tcccaggcct
     8161 cgagggggcg caggagacgg gctttgcgca gtttcacggc cttcggaacg ggcacctcgc
     8221 cctccggcgc ggcaggcgcg aagccgtcct gccagaccag gcggctgacg cgcccgccgg
     8281 gatcgggcac ggcgacgaac gtggtgcccc cggaggcggt ggcgaagagc cggccgacgt
     8341 agctctcggg ctgccgctcc gcccgcgact ggaggtcggc ctcggcgagg agagagacct
     8401 gagagcgggc gacatcccgg ttgggctgga gcagcagctc cacggtctcg cggtaacggc
     8461 ctccgggcag gccgaagcgg agcggattgg cacgcagcca cgccagggcg cggccggcca
     8521 gcggcattcc ccgccgcacc aggagctgca cgtcgttctt cgagctcttc tgctccaccg
     8581 catcgccgta ctcggtccac accaggggct tgagcttccc cgccagggtg tcggcgcgca
     8641 ggaaggcgct gttgcgaatc atgttggcga tataaagaac cagcaggagc aggagcagga
     8701 gggccaggcc caggaggaca cgggggccgt agcaggccca gaagccggcg ggctccacat
     8761 cgatgcgcac cggcacgagg atcggctccg gcgcgcgagc gcccgggggc aaaggctggc
     8821 tcccggccgg tgccagtccg agacgggtgc ggtaggtgcc gccggcgcag cagcggtccg
     8881 gccgcgcccg cagacgcagc ggcggccccc cggggctggc gatgaaatcg tggacgagga
     8941 gggccccccc ctcgccgtcc tccagcaggt ccttgcggcg ctcgccggca aaaccgagcc
     9001 ggaggttctc gggatcctcg ggccaggctt ccgcgtcggc ggggccggcc accaccgccc
     9061 gcagcggcac ctcctgctcg aagttgccgc gcacgggcac ggcgagatcg gtagcctgcc
     9121 ctcctcgtac cgcgacggtc agctccgggg ggacggagat ctggtagtcc acgtggaagc
     9181 ggaccggcag caccacggcc gggatccggc gccctccggc gctctcgccg aagaccaggc
     9241 gcacggcgct gttgaagctg cgcgagaacg gccgcggccc gcaataggga tgggcgcgca
     9301 gcgtcagcgt gtaggggttg ttgaccagga tcggctgcgc cttgccctcg ggctccttgc
     9361 ccagctcgac ggtgatgcag gagggcagct ccgcccgact gtcgagctcg gcatggccgg
     9421 gggtctgagg gaaggagcca cccagcgtga ggcgccgcgc ggcacggcca ccgggccgca
     9481 gacgaccgaa gtcgaagttg ccgggccgcg gctcgatggt gagggacgag acgaccagag
     9541 aaaccggtgc tcctctgagg gtcacgtcgt cgccggagga gagccgcagc gtcaccaccg
     9601 gctggagctc gtaatcgccc cgcgtcaggt tgctgcgcag gagcccgaag cgagcttggc
     9661 cgccagggag ctggttggcc gccacctcca ccggtggcgt accgggggcg tcggggcgcc
     9721 ggacccgtac gctggctgtg aggtctcccc ccgtcacctc gccaccgacg gcctcgccgg
     9781 cgtcattcac gagttcggcc acggcgcaga cggtcgaccc ggtctcggcg cctccgcccg
     9841 tcgccggtcc cggctgctcg caggtaccct ggaggacggc caggcgaacg gtcagccggt
     9901 actcgggcag ggccaccacc ttccaggtct cccccgcgcc ctggacgtcc acctccaccg
     9961 gctcggcgtc cggacggtag tccagggcgg cgtagcggaa cgcccggccg ttgccgtagc
    10021 ggtgggtgcc ggtccggggc tggccggcgg tctggggcga gcctccgcgg gggttgcgga
    10081 ccgagaagga gagatccgca ccgctgccag gagcgatggc gaggagacgt actcgttcgg
    10141 cgccacggtg ggcggcgagg tgccggctct gcggcgagag caggtacggc tcgtagccct
    10201 gggagcgcga cagggcggcg gcgaaggtct ggaggatgga cgccgggtct cccggattgg
    10261 ccgtcaccgc ctgtccgccg gtcctctcgg cgaggtcggc caggcccggt gaggagcgct
    10321 taccgcggaa tcccagcaga tagaactgga agagcccctc gtcgcgggag cgcagcccac
    10381 ggaggaagcc gtcgacggaa ggctcctcgt ccttgggcgt gcactcgccg tcggtgagga
    10441 gcatcaccac ctgggagaca ccggggcgct gcgcctcctg gaggatgcgg tggaccgccc
    10501 ccagagcaga cccgcagggg gtgttctttg cctcgtagtg ggccagcccg ccgtcgaggt
    10561 cgagcaggtc ctcgatcttt ttccggttct ggcgtagcgg ctcgatgggc ggcaccggcg
    10621 cgccgtcgcg cggcccgttg aggcgcacca gggagacctc gtcgcggtca tcgagcatcc
    10681 caaccagcgc ctgcaccgcg aacaccgcca agcggtccgg gtcggtctcc ttcatgctcc
    10741 ccgagtcgtc gacgatgaag acgaactggg cggcccgcgc cccggaagag gagatctcgg
    10801 actgggcagc ggcctcgaag gccaggagag aaaggccgag gagcaacgcc aggcgcagca
    10861 ttgcctggtt tggcacgttc atgagtcgcc tccccggcag tcgctcaccg gcggggtggg
    10921 attgagctcg aatttcgaga agtcgttcga ctgaaacccc aagtcgggat cgctatcgag
    10981 gatgttggag agacagagta atagcggcac gttgggctcc cgatcgttca ccgcgagcag
    11041 catcaatgct cgttgagacc c
//
LOCUS       DASPJI010000514         4751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_665109, whole genome shotgun sequence.
ACCESSION   DASPJI010000514 DASPJI010000000
VERSION     DASPJI010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665109"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4751
                     /locus_tag="VEL74_13895"
     CDS             <1..>4751
                     /locus_tag="VEL74_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63669.1"
                     /translation="GTAKFDLALYLEEQGGGLSGLLEYNRDLFDAATAVRLLDAWERL
                     VKAMPRAPESRVSELPLLSAAELQQVRTELAGEEGEVPPEVLVPRRIAVRAAAAPERL
                     AVSAPGRAVTYGELDRRAGRLARRLRALGVKPEVRVAVCLDRSPELIVALLGIWKAGG
                     VYVPLDPSQPEERLAWIMGDCGAAVLLASWRGPVPPPVGTPAVLFLDDLRGGGEEALE
                     GEVVPLLPESAAYLIYTSGSTGRPKGVVVSHGALAAYAEAMAGFYRIGPGDRVLQSAS
                     PGFDLSLDEIVPCLAGGAELALRDDAMLASAAAFFGGCGERGLTVVSLPTAVWHEIVA
                     DLGAMEGAAPPSLRLVILGGERLLPDRLATWRRRFAGHPRLLNTYGPTEATILVTASD
                     LSGPVEASEREAPLGRPVSGAGIWLLGRHGEPVPPGAPGEIAIGGPFLARGYLGQPDR
                     TAERFVPHPSSSVPGARVYRTGDLARLRSGVSGVSGGMEFAGRIDDQVKVRGYRVEPG
                     EVEAVLAGHPGIAAAVVVPREEGGRKRLVGYVVPRHPAPSAAEVRVFLAGRLPEPLVP
                     SELVFLDALPLTPHGKVDRRALPASAPSNSGEGAAVAVAPRDAAEGEVAAIWREVLGR
                     ERVGIHDNFFDLGGHSLLLARVHVLLRERLGREVPMVDLFRYPTVASLARHLMPSGPP
                     PAAPLPGPWGLEAESVQARLSPANADFLRFAAGHPEALESASFAGLAEHAAGSPYPLQ
                     PWPVLVDRARVEEMERVSVGLVRLVKSLPRRVFGNDPERLRDFYGLASADLARNLVAE
                     PSGLAEVIGRGDFLDTAGGLKCLELNLVSDLGGWQAPLWAEGYSRVPLWERFVREQGL
                     RVACRDTVALLFEHVVSAARELGRDDGAVNAAMVLPAGAGSLGPELEAWLAARYAAAL
                     RRAAPLREGMLLILSYAGLRERQGMLWAGDRRVHAAVEIHHEGTAAQALRCFKAGTLA
                     LFNAPVRAILTDKRNIALISELADRGEILTPAERALVQRHVPWTRRLAAAEASWRGGT
                     AWLPRLALEARHELVLKRAREGRGAAVHLGASTPEAVWLERVEQALADGDWVVQERVE
                     PLPSVHQRGERGWDLHDAVWGLFVFGERYGGAFLSLQPREQGRVVNLTQGASAGVVFE
                     VLKTPAAAEPAARDVPERGVAIVGMAGRFPGAADVERFWENLVAGVESVRFFPREELA
                     AAGVDPKLLDDPAYVPARAVLEGVELFDAELFGVSPREAETMDPQHRLLLECAWEALE
                     HAGYDPRRYDGPVGVYAGAGPNTYLLFNLASDRESLAAVGPVQAMLGNGADFLATRLS
                     YKLGLRGPSLTVQTACSTSLVAVHLATQALLHGECDMALAGGVRVSVPQRAGYLYQPD
                     GILSPDGHCRPFDAGAQGAVDGEGAGIVVLKRLADALEDGDHIHAVILGTAVNNDGAG
                     RVGYTAPGVDGQAGVIAAAQAVAGVRPETVGLVEAHGTATPLGDPVEVAALRQVFEAG
                     PARRGSCALGSVKGNVGHLDTAAGVTSLIKAALALERGVIPPTLHFQRPSPLLELEES
                     PFYVPAEPRAWPAGPRPRRAGVSSFGMGGTN"
BASE COUNT          536 a         1709 c         1823 g          683 t
ORIGIN      
        1 cggcaccgcc aagttcgacc tcgccctcta tctggaggag caggggggcg gcctctcggg
       61 acttctggag tacaaccggg acctcttcga cgctgccacc gcagtccgcc tcctggatgc
      121 ctgggagcga ctggtgaagg ccatgccccg ggcgccggag agccgggtgt cggagctgcc
      181 gctgctctcc gcggctgagc tccagcaggt gcggacggag ctggcggggg aggaggggga
      241 ggtcccgccg gaggttctgg tgccacggcg gatcgccgtt cgtgccgccg cggccccgga
      301 gcgcctcgcg gtgtcagcgc caggaagggc cgtcacctac ggggagctgg atcggcgggc
      361 ggggcgtctc gctcgccggc tgcgggcgct gggagtgaag ccggaggtcc gggtggccgt
      421 ctgcctggac cgttcgccgg agctgatcgt cgccctgttg ggcatttgga aggccggcgg
      481 cgtctacgtg cctctggacc cctcccaacc ggaggagcgg ctggcctgga tcatgggcga
      541 ctgcggggcc gcggtgctcc tggcctcctg gcgagggccc gtgccgccgc cggtgggaac
      601 tccggccgtg ctcttcctgg atgacctccg gggaggaggc gaggaggccc tggagggcga
      661 ggtagtgccc ctgctgccgg agagcgccgc ctaccttatc tatacctcgg gctccacggg
      721 ccggcccaag ggagtggtgg tctcccacgg cgcgctggcg gcctacgcgg aggccatggc
      781 cggcttttac cggatcggcc ctggcgaccg ggtgctccag agtgcctctc ccgggttcga
      841 cctcagcctc gacgagatcg tgccctgcct cgccggtggg gcggagctgg cgctgcgcga
      901 cgacgccatg ctggcctccg ccgcggcctt cttcggcggc tgcggcgagc gggggctcac
      961 ggttgtcagc ctgcccaccg ccgtctggca cgagatcgtg gccgacctcg gcgccatgga
     1021 aggggcggcg ccgccatcgc tccgcttggt gatcctcggc ggcgagcggt tgctgccgga
     1081 ccggctggcc acttggcggc ggcgcttcgc ggggcatccg cgcctgctca acacctacgg
     1141 gcccacggag gccaccatcc tggtgacggc gtcggacctg tccgggccgg tggaagcctc
     1201 ggaacgggaa gccccactgg gccgaccggt atccggcgcc gggatctggc tgctgggacg
     1261 ccacggcgag ccggtgccgc ccggcgcgcc gggagagatt gccatcgggg ggcccttcct
     1321 ggcgcgcggc tatctcggcc agccggaccg caccgccgag cgcttcgtcc cgcatccgtc
     1381 ctcctccgtg cccggcgccc gcgtctaccg caccggcgac ctggcccgcc tccggtccgg
     1441 cgtttccggc gtttctggcg ggatggagtt cgcgggacgg atcgacgatc aggtcaaggt
     1501 gcgcggctat cgggtcgagc ccggcgaggt ggaggcggtg ctcgccggcc atccggggat
     1561 cgcggcggcc gtggtcgtgc cccgggaaga gggagggagg aagcggctcg tgggctatgt
     1621 cgtcccgcgg catcccgctc cgtccgccgc cgaggtccgc gtcttcctgg ccgggcgcct
     1681 ccccgagcct ctggtcccct cggagctggt cttcctcgac gccctgcccc tgacgcccca
     1741 cggcaaggtg gaccgccggg cgctgccggc ctccgccccc tccaacagcg gcgagggcgc
     1801 ggcggtggct gtggcccccc gcgacgcggc ggagggggag gtggccgcca tctggcgcga
     1861 ggtgctgggc cgggaacggg tgggcatcca cgacaacttc ttcgacctgg gcgggcactc
     1921 cctgctcctg gcccgggtcc acgtgctgct gcgggagcgg ctcggccgcg aggtccccat
     1981 ggtggacctc ttcagatatc caaccgtggc ctccctcgcc cgccacctca tgccctccgg
     2041 tccgccgccc gccgccccgc ttcccgggcc gtgggggctc gaagccgagt ccgtgcaggc
     2101 gcggctgtcc cccgccaacg cggacttcct gcgcttcgcc gcaggccacc cggaggccct
     2161 ggagagcgcc agcttcgccg gcctggcgga gcacgcggcc ggcagcccct atcccctcca
     2221 accgtggcct gtcctggtgg accgcgcccg ggtggaggag atggagcggg tgagcgtcgg
     2281 cctggtgcgc ctggtcaaga gcctgccgcg gcgggtcttc ggcaacgacc ccgagcggct
     2341 gcgggacttc tacggcttgg cgagcgccga tctggcccgg aacctggtgg cggagcccag
     2401 cggcctcgcc gaggtcatcg ggcggggcga cttcctggat acggccggcg gcctcaagtg
     2461 cctggagctc aacctggtga gcgacctcgg cggctggcag gcgccgctgt gggcggaggg
     2521 gtacagccgt gtccccctct gggagcggtt cgtgcgcgag caggggctcc gcgtcgcctg
     2581 ccgcgacacg gtggcgctgc tcttcgaaca tgtggtgagc gccgcgcgcg agcttggccg
     2641 ggacgacggc gcggtcaacg ccgccatggt gctccccgcg ggcgcaggct ctttggggcc
     2701 ggagctggag gcttggctcg ccgcccgcta cgccgccgcc ctgcgccgcg ctgctccctt
     2761 gagggagggg atgctgctca tcctctccta cgccgggctt cgggagcggc aggggatgct
     2821 atgggccggc gaccggcggg tacacgctgc ggtggagatc caccacgagg ggacggcggc
     2881 ccaggccctg cgctgcttca aggccggcac gctggcgctg ttcaacgcgc cggtgcgggc
     2941 gatcctcacc gacaaacgca acatcgccct gatctccgag ctggccgacc gcggcgagat
     3001 cctcaccccc gctgagcggg cactggtcca gcgccacgtg ccgtggaccc ggcgcctcgc
     3061 cgccgccgag gcctcctggc gaggaggcac cgcctggctg ccccggctgg ccctggaggc
     3121 gcggcacgag ctggtcctca agcgggcgcg ggagggccgg ggcgcggccg tccacctcgg
     3181 cgcctcgacc cccgaggcgg tgtggctgga gcgggtggag caggccctcg ccgatgggga
     3241 ttgggtcgtt caggagcggg tcgagcccct cccctcggtc caccagcggg gcgagcgcgg
     3301 gtgggacctc cacgacgcgg tctgggggct cttcgtcttc ggcgagcgct acggcggcgc
     3361 cttcctgagc ctccagccgc gcgagcaggg gcgcgtggtc aacctcaccc aaggggcgag
     3421 cgccggggtg gtcttcgagg tgctgaagac accggccgcc gccgagccgg ccgcccgcga
     3481 cgtgcctgaa cggggggtcg cgatcgtggg catggccggc cgcttccccg gcgccgccga
     3541 cgtcgagcgg ttctgggaga acctggtggc cggggtcgag tcggtgcgct tcttcccccg
     3601 cgaggagctg gccgcggccg gtgtcgatcc caagctcctg gacgacccag cctacgtgcc
     3661 ggcccgcgcg gtgctggagg gggtcgagct gttcgacgcc gagcttttcg gggtgagccc
     3721 ccgcgaggcc gagaccatgg acccgcagca ccggctgctc ctcgagtgcg cctgggaggc
     3781 gctggagcac gccggctacg acccgcgccg ctacgacgga ccggtggggg tctacgccgg
     3841 cgccgggccg aacacctacc tccttttcaa cctcgcctcg gaccgcgaga gcctggccgc
     3901 cgtcgggccg gtccaggcga tgctgggcaa cggcgccgac ttcctggcca cccggctttc
     3961 ctacaagctg gggctgcgcg ggccgagcct gacggtgcag accgcctgct ccacctccct
     4021 cgtcgccgtg cacctggcga cgcaggcgct gctccatggc gagtgcgaca tggcgctggc
     4081 cggcggcgtg cgggtgtcgg tgccgcagcg ggcgggctat ctctaccagc ccgacggtat
     4141 cctctccccg gacggccact gccggccgtt cgacgccggc gcccagggcg cggtggacgg
     4201 cgaaggggcc gggatcgtag tgctcaagcg cttggccgac gctctggagg acggcgacca
     4261 catccacgcg gtgatcctgg gcacggcggt caacaacgac ggcgccggcc gggtgggcta
     4321 cacggcgccg ggggtcgacg gccaggccgg ggtcatcgcc gccgcccagg ccgtggcagg
     4381 cgtccgccct gagaccgtcg gtctggtgga ggcccacggc acggccacgc cgctgggcga
     4441 tccggtggag gtggcggcgc tacgccaggt cttcgaggcc ggcccggcgc gccggggctc
     4501 ctgcgccttg gggtcggtga agggcaatgt cgggcacctg gacacggcgg ccggggtgac
     4561 gtcgctgatc aaggcggctc tggccctgga gcggggggtg attccgccga cgctccactt
     4621 ccagcggccc agtcccttgt tggagctgga ggagagcccg ttctacgtcc ccgccgagcc
     4681 tcgggcctgg ccagcgggcc cgcggcctcg gcgcgccgga gtcagctcct tcggcatggg
     4741 gggcaccaac g
//
LOCUS       DASPJI010000515         4279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_842001, whole genome shotgun sequence.
ACCESSION   DASPJI010000515 DASPJI010000000
VERSION     DASPJI010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842001"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..789
                     /locus_tag="VEL74_13900"
     CDS             94..789
                     /locus_tag="VEL74_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002940511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HYG63670.1"
                     /translation="MPRRFDLIVFDWDGTLMDSLATIVACSRAVMRELELGDLLEPDI
                     RRTIGLGLRESMELLVPGCDDALYARILQSYRKHWLASYRDEPVLFEGVAGMLRELAG
                     DGYLLAVATGKGRRGLDHDLAQTCLTGVFHATRTVDEAFSKPHPKMLLDILDELGVKP
                     GRAVMIGDTVYDLEMARSAGTAAVGVCSGSHCREDLERLQPVACLETVVQLREWLGSP
                     TASASPPATSPAP"
     gene            complement(746..1603)
                     /locus_tag="VEL74_13905"
     CDS             complement(746..1603)
                     /locus_tag="VEL74_13905"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HYG63671.1"
                     /translation="MRRLPISAAALACVAALQLTASAAVAQPLNLPRPSPKASVMQSV
                     GVTDVSLHYSRPGVKGRTIWGELVPYNEVWRTGANENTTITFSTPVKIQGKELPAGTY
                     GLQTIPTTGDWTVIFSRDAELWGAFNYKPENDALRIQVKPEPAELQERMGFEFEDVTD
                     NSAKVVLRWEKIAVPFTIEVDTPKLVSESARASLRWQNPFQAANYCIQNNTCLDEAGR
                     WLDASIALQENFSNQRAKAMLLAKRNDYKGAVTAGERALAAAKSAQQPPPAEMVKELE
                     TSLADWRKR"
     gene            complement(1682..2800)
                     /gene="hemH"
                     /locus_tag="VEL74_13910"
     CDS             complement(1682..2800)
                     /gene="hemH"
                     /locus_tag="VEL74_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HYG63672.1"
                     /translation="MTYFGQPDFQHGEVPAVGVLLANLGTPDAPDTPALRRYLRQFLL
                     DPRVIELPRWKWWLVLHLFILPRRPQQSAKLYQKVWTAEGSPLLVISRRQAAAIQEVL
                     RKESATPVHVALGMRYGNPSIPAALRELAEKGCRRVLVLPLFPPYAAVTAGSTFDAVA
                     AELATWRWVPEVRTIHQYHDDPAYIAALAASIREAWRDGERGEKLLFSFHGIPKRYFL
                     AGDPYFCQCHKIARLTAEALELPRDRWEVSFQSLFGKEEWLKPYTDKTIEAMARSGIR
                     RLDVVCPGFSADCLETIEEMDEMNREVFLHAGGEHYRYIPALNDRPDHIRVLSDLVLR
                     NLQGWVEPVDLRAAEESRHRAEEMKARPVLVDAGYGRT"
     gene            complement(2826..4031)
                     /locus_tag="VEL74_13915"
     CDS             complement(2826..4031)
                     /locus_tag="VEL74_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HYG63673.1"
                     /translation="MSNTKPRIAILGAGPVGLEAALAATESGLAFTIYEAAPAVAGNV
                     RAWGHVRLFTPWELNVSPRMRRHLEAAGVAVPDGPECPTGHELADRLLEPLAALPEIA
                     PHLRLGTRVVSVGREGLLKHEEIATAERGRRPFRLLLSDASGREWAERADVVIDATGT
                     FGHPNSLGDAGIPAPGERALDAEIRRQIPDLSREAADWAGRTVLVAGTGHSAQTAVRD
                     LAALAGQAPGTRVIWALRHAEPDWGTHEGDPLADRAALVADARALADGASPAVEARRG
                     VVVEEIAKPNGKFRVVLRNGAGAETVEVDRVLSLTGFVGDHQMYRQLQVHECYATCGP
                     IKLSAALLGAGSADCLAQTSHGADTLANPEPGFFILGIKSYGRNNTFLMRVGWEQVGE
                     VFSLVGGAA"
     gene            complement(4056..>4279)
                     /locus_tag="VEL74_13920"
     CDS             complement(4056..>4279)
                     /locus_tag="VEL74_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYG63674.1"
                     /translation="DTRVWYALLDTRQGEMEGQLGVELVPLVYDHRSLAAEMRQEGLP
                     EEFVETVLTGWWTTCLEILPARERAASKF"
BASE COUNT          604 a         1450 c         1568 g          657 t
ORIGIN      
        1 cggtcgtgca cccggtcagc cgccagtcga tcacgttcga agcgccgctg cccgtcgagc
       61 tcagtacatt cctcgaacgg ctctcgctgc cggatgccca gacggtttga tctgatcgtc
      121 ttcgactggg acggcacgct gatggactcg ctggcgacca tcgtcgcctg ctcccgggcg
      181 gtgatgcggg agctggagct tggggatctg ctcgagccgg acatccgccg caccatcggc
      241 ctgggcctgc gggagagcat ggagctcctg gtgccgggct gcgacgacgc cctctacgcg
      301 cggatcctcc agagctaccg gaagcattgg ctcgccagct accgcgacga gccggtgctg
      361 ttcgaggggg tggccggaat gctgcgggag ctggccggcg acggctacct cctggcggtg
      421 gccaccggca agggccgccg cggcctcgac cacgaccttg cgcagacctg cctgaccggc
      481 gtcttccatg ccacgcgcac cgtcgacgag gcgttctcca agccccatcc caagatgctg
      541 ctggacatcc tcgacgagct gggggtgaag cccggccggg cggtgatgat cggcgacacc
      601 gtctacgatc tggaaatggc gcggagcgcc gggacggcgg cagtgggagt ctgcagcggc
      661 tcccactgcc gggaggatct ggagcgtctc cagccggtgg cctgtctgga gacggtggtc
      721 cagctccggg agtggctggg tagccctacc gcttccgcca gtccgccagc gacgtctcca
      781 gctccttgac catctcggcc ggcgggggct gctgcgcgga cttggcggcg gccagcgccc
      841 gctcgcccgc ggtcacggca cccttgtagt cgttccgctt cgccagcagc atggccttcg
      901 cccgctgatt ggagaagttc tcctggaggg cgatggaggc atcgagccag cggccggcct
      961 cgtccaggca ggtgttgttc tggatgcaat agttggcggc ctggaagggg ttctgccagc
     1021 gcaggctggc gcgggccgac tccgagacca gcttcggggt gtccacctcg atggtgaacg
     1081 gcaccgcgat cttctcccag cgcagcacca ccttggccga gttgtcggtg acgtcttcga
     1141 actcgaagcc catgcgctcc tggagctcgg cgggctcggg cttcacctgg atgcgcagcg
     1201 cgtcgttctc cggcttgtag ttgaaggcgc cccacagctc ggcgtccctg ctgaagatca
     1261 ccgtccagtc gccggtcgtc gggatggtct gcaggccgta ggtgccggcg ggcagctcct
     1321 tgccctggat cttgaccgga gtgctgaagg tgatggtggt gttctcgttg gcgccggtgc
     1381 gccagacctc gttgtagggg accaactcgc cccagatcgt gcgccccttc acgccggggc
     1441 ggctgtagtg gaggctcacg tcggtgacgc cgacgctttg catgacgctc gccttcgggc
     1501 tggggcgcgg caggttgagc ggctgggcca cggcggccga ggcggtcagt tgcagagcgg
     1561 cgacgcaggc cagggcggcc gcggagatcg gaagccttct catctcgttc ctccttctaa
     1621 acgttgaaac cgttgaacgg ctcgacagga aggagaaatc taccagagtg ggccgccgga
     1681 ttcaggtccg tccatagccg gcgtcgacga ggaccggccg cgccttcatc tcctcggcgc
     1741 ggtggcggga ctcttcggcg gctcgcaggt cgaccggctc gacccacccc tggaggttgc
     1801 gcaggacgag gtcggacagc acccgtatgt ggtcggggcg gtcgttgagc gccgggatgt
     1861 agcggtagtg ctcgccgcca gcgtggagga agacctcgcg gttcatctcg tccatctcct
     1921 cgatcgtctc caggcaatcg gccgagaagc cggggcagac gacgtcgagg cggcggatgc
     1981 cggagcgggc catcgcctcg atcgtcttgt ccgtgtaggg cttgagccac tcctccttgc
     2041 cgaacagcga ctggaagctg acctcccagc ggtcgcgggg gagctccagg gcctcggcgg
     2101 tgaggcgggc gatcttgtgg cactgacaga agtaggggtc gcccgccaga aagtagcgct
     2161 tcggaatgcc gtggaaggag aagaggagct tctccccgcg ctcgccatcc cgccaagcct
     2221 cgcggatgct cgccgcgagc gccgcgatgt aggccggatc gtcatggtac tgatggatgg
     2281 tgcgcacctc gggcacccag cgccaggtgg cgagctcggc ggccacggcg tcgaaggtgg
     2341 agccggcggt cactgccgcg tagggcggga agaggggcag gaccaggact cggcggcagc
     2401 ccttctccgc cagctcgcgg agcgccgccg ggatcgacgg gttgccatag cgcatcccga
     2461 gagccacgtg caccggggtg gccgactcct tccgcagaac ctcctggatc gccgccgcct
     2521 gccggcgcga gatgacgagg aggggggagc cctccgcggt ccagaccttc tggtacagct
     2581 tcgccgactg ctgcgggcgc cgggggagga tgaacaggtg gaggacgagc caccatttcc
     2641 accgcggcag ctcgatgacg cgcgggtcga gcaggaactg gcgcaggtag cggcgcagcg
     2701 ccggcgtgtc cggcgcgtcc ggggtgccga ggttggcgag cagcaccccc accgcgggga
     2761 cctcgccgtg ctggaagtcg ggctggccga agtacgtcac catggctcgc cgagcataca
     2821 gcggctcagg ccgcccctcc caccaggctg aacacctccc ccacctgctc ccagccgacg
     2881 cgcatcagga aggtgttgtt gcggccgtac gacttgatgc ccaggatgaa gaagccgggc
     2941 tccgggttgg cgagggtgtc ggcgccgtgg ctggtctggg cgaggcagtc ggcgctcccc
     3001 gcgcccagca gggccgccga gagcttgatc gggccgcagg tggcgtagca ctcgtggacc
     3061 tggagctggc gatacatctg gtgatcgccg acgaagccgg tgagggagag gacgcggtcc
     3121 acctccacgg tctcggcgcc ggcgccgttg cggagcacca cccggaactt gccgttcggc
     3181 ttggcgatct cctccaccac cacgccgcgc cgcgcctcga ccgccggcga ggcgccgtcc
     3241 gccagcgccc tggcgtccgc gacgagggcg gcccggtccg cgagggggtc gccctcgtgg
     3301 gtgccccaat ccggctcggc atggcgcagc gcccagatca cccgcgtccc cggcgcctgg
     3361 ccggccaggg cggcgaggtc acgcaccgcg gtctgcgccg agtggccggt gccggcgacg
     3421 agcacggtcc tgccggccca gtcggcggcc tcgcgggaga ggtccgggat ctggcgccgg
     3481 atctcggcat ccagggcccg ctcacccggg gcggggatgc cggcgtcccc caggctgttg
     3541 ggatgaccga aggtgccggt ggcgtcgatg accacgtcgg cgcgctcggc ccactcgcgg
     3601 ccggaggcgt cggagaggag caagcggaag gggcgccggc cgcgctcggc ggtggcgatc
     3661 tcctcgtgct tgaggagccc ctcgcggcct accgagacga cgcgggtgcc cagccgcagg
     3721 tggggggcga tctccggcag cgccgccagg ggctcgagca gacggtccgc cagctcgtgc
     3781 ccggtggggc actccggccc gtccggaacg gcgacgcccg ccgcttcgag gtggcgccgc
     3841 atgcgcggcg agacgttcag ctcccacggg gtgaacagcc gcacgtgccc ccaggcgcgg
     3901 acgttgccgg cgaccgcggg cgcggcctcg tagatggtga aggcgaggcc gctctctgtg
     3961 gcggcgaggg cggcttcgag ccccacgggc ccggcgccca ggatggcgat gcggggcttg
     4021 gtgttggaca tcctcttcta tctcctctcg gaccgtcaga acttggaagc cgcccgctcg
     4081 cgggcgggca ggatctcgag acaggtggtc caccagccgg tgaggaccgt ctccacgaac
     4141 tcctcgggga gcccctcctg ccgcatctcg gcggcgagcg accggtgatc gtagaccagg
     4201 gggaccagct ccacgcccag ctgcccctcc atctcccctt ggcgggtgtc cagcagcgca
     4261 taccagacgc gggtgtcgc
//
LOCUS       DASPJI010000516        10218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_434080, whole genome shotgun sequence.
ACCESSION   DASPJI010000516 DASPJI010000000
VERSION     DASPJI010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10218)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10218
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434080"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..250)
                     /locus_tag="VEL74_13925"
     CDS             complement(32..250)
                     /locus_tag="VEL74_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63675.1"
                     /translation="MAEVNLSTIGEERLKALLKAAFVEVLEERRDLVLDVMEEALEDI
                     ALGRAIQEAEGSEMVGEDSILAILHETD"
     gene            complement(270..524)
                     /locus_tag="VEL74_13930"
     CDS             complement(270..524)
                     /locus_tag="VEL74_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010798935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63676.1"
                     /translation="MKQREVQDSGNVTWTCVQALSGLSGEAAAEAAEKIEIDEGTVPV
                     VCTPSGGEQSVRIELPKDWSERMPDEELLEAIGSAKSGEV"
     gene            complement(991..1727)
                     /locus_tag="VEL74_13935"
                     /pseudo
     CDS             complement(991..1727)
                     /locus_tag="VEL74_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016856065.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
     gene            complement(1731..2354)
                     /locus_tag="VEL74_13940"
     CDS             complement(1731..2354)
                     /locus_tag="VEL74_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63677.1"
                     /translation="MEPPSQTESFADKAVDEDGWFFTAEDLLAAAKALEPAMEGYWRL
                     QRERAQLEDSEFRVFDYEPDVRGPYLMLTAYAIENFCKAVVMCDSWWLIFEKKELPRK
                     FTKHDLLELLKRVGIPLSSEDEELAFRLERSAVWAGRYPVPTHPAHRTLYFTRARGEK
                     VVPSWHREDDVKLAKDLVERVRDFVVKELEARHQAEINRALHPRSED"
     gene            2679..3209
                     /locus_tag="VEL74_13945"
     CDS             2679..3209
                     /locus_tag="VEL74_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63678.1"
                     /translation="MRDSEEGNTRRLSNAFAQEFLEIDEERDDSPGGRDAEGEGPFEI
                     FPASGWPEWPDAHGIWAPGERPERGDRPAFVAAGRPPALFSAIVRPIIGCDPFYWLGR
                     DKWRGGYPLLRDGEPAAWMEIFHPEWALGVSLLEAMGRQPRDVALFLEAIGPTCRRRA
                     GAILLERLESGSGSGG"
     gene            3412..3684
                     /locus_tag="VEL74_13950"
     CDS             3412..3684
                     /locus_tag="VEL74_13950"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HYG63679.1"
                     /translation="MDVPVIRYPPEILEALQESPEEFEREARLLLALKLYEMEKLSSG
                     LAARLAGVPRSKFLFLLGQHGLSPSGQAPDELAEDLQDARRARRRG"
     gene            3845..4306
                     /locus_tag="VEL74_13955"
     CDS             3845..4306
                     /locus_tag="VEL74_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63680.1"
                     /translation="MPNRPAPPPSRRPAAPPPGYATVPRIDPRQWEHFAVFRETFLAT
                     LDDEDAVEAFRVFAKAQYTMALEYGPPWYGTPADATAHGLRAITADLRFLQGYLTHVG
                     NLEQSALDQGLITPRNERWLRFAGRMSEKLRLIGDEIERELEAGLAEKDEG"
     gene            4411..4764
                     /locus_tag="VEL74_13960"
     CDS             4411..4764
                     /locus_tag="VEL74_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63681.1"
                     /translation="MKTKRLYPMFAVLLLMTAGAALAGEMPVQTQVTAPDPAAMALEV
                     PAEPDPGVQLAPGAEIPEWLRDIDPKVTLSKAGGTYSWGGCQCYTNCSSRYSCICSHD
                     GCCSGCCSAAFGALC"
     gene            4888..5013
                     /locus_tag="VEL74_13965"
     CDS             4888..5013
                     /locus_tag="VEL74_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63682.1"
                     /translation="MSSPVQVRGCCCGIPFGCGVLVFFVASVAVWQLAVADWLPF"
     gene            5040..5996
                     /locus_tag="VEL74_13970"
     CDS             5040..5996
                     /locus_tag="VEL74_13970"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HYG63683.1"
                     /translation="MRRLRAYAPASIGNVAAGFDILGAALAPLEPPAPADSSGGGLWG
                     DVVELAEGPPGLRVEGPYAHRLPPEPEENLVLRAFGLFRAALGREVVLPAVTLHKRLP
                     VKSGLGSSSASAVAALVAFNAWEGEPLGRAALLELAGRTEELAAGAPHLDNVAPSLLG
                     GLRLVTDDGAPRALPFPDGLLFAVVHPDLELATAAARAVLPRQVPLADAVCFAQNLAG
                     FVHALDRGDLGLLRRCLRDPLAEPFRAGLVPGFREAQRAALAAGALGCSLSGAGPATF
                     AVAEAELAAGVASGMQEAFAAAGATSTARLCRLDLQGARILP"
     gene            5993..7321
                     /gene="thrC"
                     /locus_tag="VEL74_13975"
     CDS             5993..7321
                     /gene="thrC"
                     /locus_tag="VEL74_13975"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HYG63684.1"
                     /translation="MILRSTRDPDSASTAATFSDAAERSLAPDGGLYLPDPLPRFADV
                     DGLLALDFRPRSQEILDRLLGDEIPRADLAAIVEDAFDFPVPLVRIADRLFALELFHG
                     PTLAFKDFGARFLARVLRRIAERRGDTAEGQPRTVLTATSGDTGAAVAHAFWRVPGFR
                     VVVLYPRGLVSPLQERQFATLGDNVRALAVAGTFDDCQALVKACFRDPELEARHRFTS
                     ANTINIARLVAQVLYYFEAVARLPRGAGAPVISVPSGNFGNLCAGLMAGRLGLPVRAF
                     VAATNANTTVPEYLSGGEYRPRPSVATLSNAMDVGAPSNWERIEALYSGDPEALRRDL
                     RWGSAGDEGTRAALRELRDRGYVADPHAAVAYRVLTERLEPGETGVFLATAHPAKFGE
                     VLAPLGIEVPLPDALRRVANRPFLAEPLPNDLDALRAALDVDHLTGPPLG"
     gene            7340..8080
                     /locus_tag="VEL74_13980"
     CDS             7340..8080
                     /locus_tag="VEL74_13980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG63685.1"
                     /translation="MSRLDGAKVVIVGGAGGVGEGIVRVLLQRGARVLVSSRSEEKLR
                     ELAAYCEDISGGELTTVVGDLSGEETARVLQHQIFERFRELDVAVASLGGWVQGKPLT
                     SVDMATWNQVLRDNLTSHFLAIKMLVPLLHPKTGSYIHVNGFSAEQPYAMAGPVAMAS
                     AAQKSMALTLAEELKPTGIRVFELILGPVRTRARLRQGQVRSDWLTPEEIGDYVAGLI
                     TGQIGGRTDGKSEIVHRLMSKNDLLPVN"
     gene            8180..9004
                     /locus_tag="VEL74_13985"
     CDS             8180..9004
                     /locus_tag="VEL74_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63686.1"
                     /translation="MKRQYLKMAVVAAALVLAAAPAVADNVIYNGSDFWWTRGDGSTF
                     SDFSQEPIPAGFFCGKSAPFTGRIVYQGVPLNTVDGKLGKVDTIVQRMDDATFNKNGV
                     ATTRIQMRALHLESVAPIKTACGKFNVVVSLDGEQPVTRMRILRQSEDGGRFIAPVAV
                     NVKMSFVPVSGKVREPLEITRSIRFRPSVIPWAIRSNEAKAFSRSGIFLVDTDVDGRP
                     DTYLPGTSNFVAGLRSSQNKYVRNDVDVVDVWNHEDPLHEHATTTPPPTDPTTGVN"
     gene            complement(9087..9557)
                     /locus_tag="VEL74_13990"
     CDS             complement(9087..9557)
                     /locus_tag="VEL74_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020765934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYG63687.1"
                     /translation="MLKKLAQTDDSIALLLVRLTLGAVIFPHGAQKLLGWFGGDGFAG
                     AMHFFSGELKLPMVVGLLVIIAEFFGALALIFGFLGRVGAFGILCVMLGAIVLIHGKF
                     GFFMNWYGTQPAEGFEYHLLAIGMALAVIVQGSGALSIDRSLSDRSRSTGPRLP"
     gene            complement(9623..>10218)
                     /locus_tag="VEL74_13995"
     CDS             complement(9623..>10218)
                     /locus_tag="VEL74_13995"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HYG63688.1"
                     /translation="LAAAREIGYPVVVKAVAPDLVHKSEAGAVRVDLRGDGELAEAVD
                     GMERSVVAAGHRVEGFLVQEMARGGHEVIFGITTDARFGPILMFGLGGKYVEVFQDVR
                     FGLLPLAPYEALEMIRGIRGFKLLEGVRGEAGADLDILCEVLLRLAQLVQRHPRVREL
                     DINPFLAAADQGRAKALDARIRVAGPGEEAAVQDSRG"
BASE COUNT         1590 a         3552 c         3437 g         1639 t
ORIGIN      
        1 tcgcgctcaa agctcttcgt gaacagcgtc ttcaatcggt ttcgtggagg atggcgagga
       61 tagagtcctc tcccaccatc tcgctcccct ccgcttcctg gatggcgcgg cctagcgcga
      121 tgtcctccag agcttcctcc atgacgtcga ggaccagatc ccgtcgctcc tccagcacct
      181 cgacgaaagc cgccttgagg agagccttga gccgctcctc cccgatcgtt gaaagattga
      241 cctctgccat acagacctcc agctctcaac tatacctcgc cgctcttggc acttccgatc
      301 gcctccagca gctcctcgtc cggcatccgc tcggaccagt ccttcggcag ctcgatacgc
      361 accgactgct cgccgccgct cggcgtgcag accaccggca ccgtcccctc gtcgatctcg
      421 atcttctccg cggcttcggc ggctgcctcg ccggagagcc cggagagcgc ctgcacgcaa
      481 gtccacgtca cgttgccgga atcctggacc tcgcgttgct tcattcgatc ttctcctcgc
      541 ggtgatttcc gggccgccgc accgtgcgac ggcctgttgg aaggttccaa cacgttgccg
      601 aagcgaacga cgcgccatcg cagagtacga ccggccgttg tacggatcca acgcgctgtc
      661 gtcgtcgacg acgggctgcc gaagcgaacg acgcgccatc gcagagtacg acgggccgtt
      721 gtaccgatcc aacgcgctgt cgtagtggac gacgggctgc cgaagtgaac gacgggccgt
      781 cgcagagtgc gacgggccgt tgtatcgatc caacgcgctg tcgtagtgga cgatgggctg
      841 ccgaagcgaa cgacgggccg tcgcagagta cgacggacgg ttgtaccgat ccaacgcgct
      901 gtccgtcgtc gacgacgggc tgtcgaagag tacgacgcgg cgccgaacgg tacgccgagg
      961 cgtcgtatcc ttccaatttg ccgtcgtcag ctacgacgcg ccgccggagg cggcgaggtc
     1021 ccgctcatcc ttctccgtag cgagcgccct ctccaccgcc gcttcggcgt ggaggcgggt
     1081 cgtgtcgaag gtgggatggg cgagtcctcc ggccggatga gcatcccgat ctccgtgcat
     1141 cccaggatga ccccctccgc ccccagctca cccaaccgct ggatgatccg cacgtactcg
     1201 cgccgcgaat cctcccggat ctgccccctg cacagctctt cgtagatcac ccggtggatc
     1261 gcctcccggt ccggggcgtc gggcaccatg gcctcgatcc cgaagcggtc gtcgagccgg
     1321 gaacgataga agtcctgctc catggtgaag cgggtgccga ggagcgcgac gcgccggtgc
     1381 cccgcggccc ggatgcgctc cccggcggcg tccaccacat gaaggaaggg gatcgccacc
     1441 gcgccctgaa cctcgtcgaa caccttgtgc atcgtgttcg tgcccagcag ggcgaagtcc
     1501 gcgccgccgc gctccaggct ccgggccgcc tccaccagaa tctcggtggt gcggtcccag
     1561 tcacccgcgt gctgcgcgac ggcgatctcg tcgaagtcga cgctgtagag aaggacccgc
     1621 gccgagtgct gcccccccag gcgctgctgg accaggcggt tgagggcgct gtaatagatg
     1681 gcggtggatt ccgggctcat gcctccgagg aggccgatca gcttcatctc ctagtcctcg
     1741 gaccttggat gaagtgcgcg gttgatctcg gcctggtggc gtgcctctag ctcttttact
     1801 acaaagtctc gaactctttc gacgaggtcc ttagccagtt tcacatcatc ttccctgtgc
     1861 cacgagggaa cgaccttctc tcctcttgct ctcgtgaagt agagggttct atgcgctggg
     1921 tgggttggga ccggataccg gccggcccat acagcactcc gctcaagacg aaaagcgagc
     1981 tcctcatctt ctgaagacag gggaatgcca acgcgcttga gaagctctaa taggtcatgc
     2041 tttgtaaact tcctaggtag ctcttttttt tcaaagatca accaccagga gtcgcacatc
     2101 acaacagcct tacagaagtt ctcgattgca taggctgtta gcatgaggta gggtcctctg
     2161 acatctggtt catagtcaaa tactcgaaac tcagagtctt ccagttgggc ccgctctcgc
     2221 tggagacgcc agtaaccttc catggcaggt tccaaagcct tagcggcagc taacaagtcc
     2281 tcggcggtaa aaaaccaacc gtcttcgtct acagctttgt ccgcaaaact ttccgtctgg
     2341 gagggaggtt ccattgtagc catcctcata gttcaagctt tcctgaagag tcggactccg
     2401 cgatgctcac tcccatcagc actgccccca gttctcctcg ccgcgactat tgacatcctc
     2461 ccgagtccca cgctagagtc gaatcatagc gcaagtcccg gccaagttct tccgggggag
     2521 ggaggtgtcg aggcgaagag gaagatgtcg aagcagtcag ccgtcgtggt ctgagtgggg
     2581 ccgggtgtcc ggcccctgcc aaccgacagt caactgacag cggacccggg tgaggatgcg
     2641 accctcatcc gggccctatc acgcaaagga gtaaccccat gcgcgactct gaagagggta
     2701 acacccgtcg tctgtccaac gcgttcgccc aggagttcct tgaaatcgac gaggagcggg
     2761 acgattcccc gggcggccgg gatgccgagg gggagggccc gttcgaaatc tttcccgcct
     2821 ccggctggcc ggagtggccc gacgcccacg gcatctgggc accgggggag cgccccgagc
     2881 ggggggaccg tccggcgttc gtggccgccg gtcgtccgcc ggcgctgttc tcggccatcg
     2941 tccggcccat catcggctgc gatcccttct actggctcgg ccgggacaag tggcgaggcg
     3001 gctatcccct gctccgggac ggcgagcccg cggcgtggat ggagatcttc catcccgagt
     3061 gggccctggg cgtgagcctt ctggaagcga tgggccgcca gccgcgggac gtggcccttt
     3121 tcctggaagc catcggcccc acctgccgcc ggcgagcggg tgccatcctc ctcgaacggc
     3181 tggagagcgg gagcggatcg ggcgggtagc atccttatct atatagatgg gggcggcgga
     3241 ggggctggtc ggggccctcc gccgaaccgg acttcttgat gtaggggagg ggcttgcccc
     3301 ctcccgggcg gggggccctc accgtcaagg tgtggtctca atgaagcctc cgcccagccg
     3361 ggaggggaca agcccctccc ctacacagag tttgatagcg ctcaggtctt tatggacgtc
     3421 ccggtgattc gttatccccc tgagatcctg gaagcgctcc aggaatcgcc agaggagttc
     3481 gagagagagg ctcgcctact gttggcactc aagctctacg agatggagaa gctcagcagc
     3541 ggcttggcgg ctcgactggc cggagttccg cggtcgaaat tcctgttcct gctaggccag
     3601 cacggcctgt cgccttctgg ccaagctcct gacgagctcg ccgaagatct ccaagatgcc
     3661 cgacgggcga gacgtcgggg atagcacccc tttaactgac ccggtgagcg gcggggggac
     3721 ggggcggcaa ccccgggcgg atttgcgggc aagggccggt ttgggctgag ctgggtatag
     3781 gctgacgcgc gaagtcgccg gccagtccgg caacacagac ccgtctcctg gaggatcacg
     3841 cccgatgccg aaccgccccg caccccctcc gtcgcgccgt cctgccgccc cgcctccggg
     3901 gtacgccacc gtgccaagga tcgatccccg gcaatgggag cactttgccg tcttccggga
     3961 gacgttcctg gcgacgctgg acgacgagga cgcggtggag gccttcaggg tgttcgccaa
     4021 ggcccagtac accatggcgc tcgagtacgg gccgccctgg tacggcacgc cggccgacgc
     4081 caccgcccac gggctgcgcg ccatcacggc cgacctccgt ttcctccagg gctacctgac
     4141 ccacgtgggc aacctcgagc agtccgccct cgaccagggg ctgatcaccc ccaggaacga
     4201 gcgctggctc cgcttcgccg gccgcatgtc cgagaagctc cggctgatcg gggacgagat
     4261 cgagcgcgag ctggaggcgg ggctggccga gaaggacgag ggctgaaggg gggatcggcg
     4321 aagggcgcac attcatgaac tcgcaaatac gcatgattcg ggtgtaagct ctgggaggcc
     4381 cccggatacg gggctgaaag gagagagagg atgaagacga aacggcttta cccgatgttc
     4441 gctgtcctgc tcctgatgac ggccggtgcc gcgttggcgg gcgagatgcc ggtccagacg
     4501 caggtcacgg cgcccgaccc tgccgccatg gcgctggagg tcccggccga gccggacccc
     4561 ggcgtccagc tcgcccccgg cgccgagatc cccgagtggc tgcgcgacat cgacccgaag
     4621 gtgaccctgt ccaaggccgg cggcacctac agttggggcg gttgtcagtg ctacaccaac
     4681 tgcagcagcc ggtatagctg tatctgtagc cacgacggct gctgctcggg ctgctgctcc
     4741 gctgccttcg gcgcgctctg ctgatcctgc ccctgaccgg ggcgccccca gcgggcgccc
     4801 cggcctccgc tgatccatct ggaagacggc ttgcacggaa gggtgacggc tcggctcatc
     4861 gagccgctcc aggaaggagg cctttccatg tcgagcccgg ttcaggtccg ggggtgctgc
     4921 tgcgggatcc cgttcggctg cggtgtcctg gtgttcttcg tcgcgtcggt ggccgtctgg
     4981 cagctcgcgg tggcggactg gcttcccttc taaaggcgga gccgtgatct gatcccctca
     5041 tgagacggct ccgcgcctat gccccggcca gcatcggcaa cgtcgccgcc gggttcgaca
     5101 tcctgggcgc ggcgctcgcc cccctcgagc cgcccgcccc agccgattcc tccggcggcg
     5161 ggctctgggg cgacgtggtg gagctggccg aggggccgcc gggactgagg gtggaagggc
     5221 cgtacgccca ccggctgccg ccggagccgg aggagaacct ggtgctccgc gctttcggcc
     5281 tgttccgcgc ggcgctcggc cgggaggtgg tcctgcccgc ggtgaccctc cacaagcggc
     5341 tgccggtcaa gagcggcctg ggatcctcca gcgcctcggc ggtcgccgcc ctggtggcgt
     5401 tcaacgcctg ggagggggag ccgctcggcc gcgccgccct gctggagctg gccggccgga
     5461 ccgaggagct ggcggccggc gccccgcatc tggataatgt ggcgccctcc ctcctgggcg
     5521 gcctgcgcct agtgacggac gacggcgccc cccgggcgct gccgttcccc gacggcctgc
     5581 tgttcgccgt ggtccacccg gacctggagc tggccaccgc ggcggcgcgg gcggtgctgc
     5641 cgcgccaggt gccgctggcc gacgccgtgt gcttcgcgca gaacctggcc ggcttcgtcc
     5701 acgccctgga ccgtggcgac ctcggcctcc tgcgccgctg cctgcgcgac ccgctggcgg
     5761 agccgttccg cgccggcctg gtgccgggct ttcgggaggc ccagcgggcg gcgctggcag
     5821 ccggcgccct ggggtgcagc ctctcgggag ccgggcccgc gaccttcgcc gtggccgagg
     5881 ccgagctcgc ggcgggcgtg gcctcgggga tgcaagaggc cttcgcggcg gccggagcca
     5941 cgagcaccgc ccgcctctgc cggctcgacc tccagggggc ccggattctc ccatgatcct
     6001 gcgcagcacc cgcgacccgg actcggcctc cacggcggcg accttctctg acgcggcgga
     6061 gcggagcctg gcacccgacg gcgggctcta cctgccagac cccctccccc gcttcgccga
     6121 cgtggacggc ctcctcgccc tcgatttccg gccgcggtcg caggagatcc tcgaccggct
     6181 gctcggagac gagatcccgc gcgccgacct cgccgccata gtagaagacg cgttcgattt
     6241 ccccgtcccc ctggtgcgga tcgcggaccg cctgttcgcc ctggagctct tccacgggcc
     6301 gaccctggcg ttcaaggact tcggcgcgcg gttcctggcg cgggtgctgc ggcggatcgc
     6361 cgagcggcgg ggggataccg cagaggggca gccccgcacg gtgctgaccg ccacctccgg
     6421 agatacgggg gccgccgtgg cccacgcctt ctggcgggtg ccgggcttcc gggtggtggt
     6481 gctctacccc cgcgggctcg tctctcccct ccaggagcgc cagttcgcca ccctggggga
     6541 caacgtgcgg gcgctcgcgg tagcggggac gttcgacgac tgccaggcgc tggtcaaggc
     6601 ctgcttccgg gacccggagc tggaggcgcg gcaccgcttc acctcggcga acaccatcaa
     6661 catcgcccgc ctcgtggcgc aggtcctcta ctacttcgaa gccgtggccc ggctgccgcg
     6721 gggcgccggg gcgccggtga tctcggtgcc gagcggcaac ttcggcaacc tctgcgcggg
     6781 attgatggcc gggcggctgg ggctgccggt gcgggccttt gtcgccgcga ccaacgccaa
     6841 cacgaccgtg ccggagtacc tgagcggcgg ggagtaccgg ccgcgcccct cggtggcgac
     6901 cctctccaac gccatggacg tgggggcgcc gagcaactgg gagcggatcg aggcgctcta
     6961 cagcggcgac cctgaagccc tgcgccggga tctgcgctgg ggctccgccg gcgacgaggg
     7021 gacgcgggcg gcgctgcgcg agctgcggga ccggggctac gtcgccgatc cccacgcggc
     7081 ggtggcctat cgcgtgctga cggagcgcct ggagccgggg gagacggggg tcttcctggc
     7141 gacggcccac cccgccaagt tcggcgaggt gctggcgccg ctggggatcg aggtgccgct
     7201 gccggacgcc ctgcgacggg tggcaaaccg gcccttcctg gccgagccgc tccccaacga
     7261 cctggacgcg ctgcgggcgg cgctcgatgt tgaccatctg acggggccac cgctaggctg
     7321 agcccagagg aggaccagca tgagccgtct ggacggagcc aaggtcgtca tcgtgggagg
     7381 ggcgggggga gtcggagagg ggatcgtccg ggtgctgctc cagcgcggag cgcgggtcct
     7441 ggtgtcgtcg cggagcgagg agaagctgcg ggagctcgcg gcctactgtg aggacatctc
     7501 ggggggcgag ctgaccacgg tggtggggga cctctccggc gaggagacgg cccgggtcct
     7561 ccagcaccag atcttcgagc gcttccgcga gctggacgtg gcggtggcct ccctcggcgg
     7621 ctgggtgcag gggaagccgc tgaccagcgt cgacatggcg acgtggaacc aggttctccg
     7681 ggacaacctg acctcccact tcctggcgat caagatgctc gtcccgctgc tccaccccaa
     7741 gacggggagc tacatccacg tcaacggctt cagcgccgag cagccctatg ccatggccgg
     7801 cccggtcgcc atggcgtcgg cggcccagaa gtcgatggcg ctgaccctgg ccgaggagct
     7861 caagccgacc ggcatccggg tcttcgagct gatcctggga cccgtccgga cgcgggcgcg
     7921 cctgcggcag ggtcaggtcc ggtccgactg gctcactccg gaggagatcg gcgactacgt
     7981 cgccgggctc attaccggcc agatcggggg ccgcaccgac gggaagagcg agatcgtgca
     8041 ccggctgatg agcaagaacg acctcctgcc ggtgaactga gaagaatagc aatttggaag
     8101 aagtcttcag ccgggtcctt gcctcatgct ctccagcggc cggccagcga ggccggtcga
     8161 ctctgtcaaa ggggatcgta tgaagcgtca gtacctgaag atggccgtcg tggcggccgc
     8221 tctcgtcctg gcggccgccc cggccgtcgc ggacaacgtg atctacaacg gcagcgattt
     8281 ctggtggacc cggggagatg ggagcacctt ctccgacttc tcccaggagc cgatcccggc
     8341 tggattcttc tgcggcaagt cggcgccctt caccgggcgg atcgtctacc agggcgtgcc
     8401 gctgaacacc gtcgatggaa agctgggcaa ggtcgacacc atcgtccagc gcatggacga
     8461 cgcgacgttc aacaagaacg gcgtcgccac cacgcggatc cagatgcggg ccctccatct
     8521 ggagagcgtc gctcccatca agacggcctg cggcaaattc aacgtcgtgg tgagtctcga
     8581 cggtgagcag ccggtgaccc gcatgcgcat cctgcgtcag agcgaggacg gcggccgttt
     8641 catcgcgccg gtcgcggtca acgtgaagat gtcgttcgtg ccggtctcgg gcaaggtgcg
     8701 cgagccgctg gagatcacgc gcagcatccg cttccggccg agcgtgatcc cctgggcgat
     8761 ccggtccaac gaagccaagg ccttctcccg ctccggcatc ttcctggttg acaccgacgt
     8821 cgacggccgc ccggacacct acctgcccgg cacgtcgaac ttcgtcgcgg gcctgaggag
     8881 ctcccagaac aagtacgtga ggaacgacgt cgatgtcgtc gacgtctgga accacgagga
     8941 tccgctccac gaacacgcta ccaccacccc gccgccgacc gatccgacca ccggcgtcaa
     9001 ctgagctcga gctcggcagg ttcttcgagg gtcccccggc cccgccacct tcgaggcggc
     9061 ggggccggtc tgctttgcgg atctccctac ggcaggcgcg gccccgtgga gcgcgaacgg
     9121 tccgagagcg agcggtcgat cgagagggcg ccgctgccct ggacgatcac cgccagcgcc
     9181 atgccgatcg ccagcaggtg gtactcgaag ccctcggccg gctgggtgcc ataccagttc
     9241 atgaagaagc cgaacttgcc gtggatcagg acgatggcgc ccagcatcac gcagaggatg
     9301 ccgaaggcgc cgacccgccc caggaagccg aagatcagcg ccagggcgcc gaaaaactcc
     9361 gcgatgatca ccagcaggcc gacgaccatg ggcagcttca gctcgccgga gaagaaatgc
     9421 atcgcccccg cgaagccgtc acccccgaac cagcccagca gcttctgggc accgtggggg
     9481 aagatgacgg cccccagggt gagccgcacg agcaacagcg cgatggagtc gtccgtctgg
     9541 gccagcttct tgagcatcga gtaccctccg tgaggcggtc tgctacgttc cggaggattc
     9601 tcgcccagag gaggcgccgc ggctagcccc ggctatcctg aaccgccgct tcctcccccg
     9661 gccccgccac ccggatgcgc gcgtccagcg ccttcgcccg cccctggtcg gcggcggcga
     9721 ggaaggggtt gatgtccagc tcccgcacgc gcgggtggcg ctggacgagc tgcgccaggc
     9781 gcagcagcac ctcgcacagg atgtcgaggt cggcgccggc ctcgccccgc accccttcga
     9841 gcagcttgaa cccgcggatg ccgcggatca tctccagcgc ctcgtagggg gcgagcggca
     9901 gcagcccgaa gcggacgtcc tggaagacct ccacgtactt gcccccgagg ccgaacatga
     9961 ggatcggccc gaagcgcgcg tcggtggtga tgccgaagat cacctcgtgg ccgccgcgcg
    10021 ccatctcctg caccaggaag ccctccaccc ggtggccggc ggcgacgacc gaccgctcca
    10081 tgccgtcgac cgcctcggcc agctcgccgt cgccgcgcag gtcgacgcgc accgccccgg
    10141 cctcgctctt gtgaaccagg tccggtgcca ccgccttgac caccaccggg tagccgatct
    10201 cccgcgccgc cgccagcg
//
LOCUS       DASPJI010000517         3577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_379306, whole genome shotgun sequence.
ACCESSION   DASPJI010000517 DASPJI010000000
VERSION     DASPJI010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379306"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3577
                     /locus_tag="VEL74_14000"
     CDS             <1..>3577
                     /locus_tag="VEL74_14000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63689.1"
                     /translation="LPELPIQYADFAVWQRQWFEGELFQEQLSYWKSQLAGLVESPLE
                     LPTDHPRPPIESFRGDLESSHLAAPLVHELKALARRHRATLFMVVLSGFNILLSRYSG
                     QQDIGIGTGIANRNRREIEDLIGFFVNTLVLRSDLSGMPSFEQFLARVRTATVEAYDH
                     QDLPFEMLIEKINPERALNRHPLVQVMLAFQNFPRSARNLPGLSLTGSDERLAQISTA
                     KFDLALFVTEPGEELGLILQYSTDLFEVHTIRRLLAHLERLLWAVVEHPEAAVTELPL
                     LTGAEQAQVLREWSDTTKICPQRPLVHELFAEHARRRPHAEAVTSWDGGRLTYGEVEA
                     RANRLAHHLRSLGVGPDVLVGVAAERTCERIVGIVAVLKAGGAYVTLDPTYPRERLAF
                     LLDDARVPVLLTEERFADALPDCTAEVIRLDADWASIRGDEVAGPASGVGPDNLAYVV
                     YTSGSTGKPKGVEIPHAGLMNLVRWHRDLYQVTPEDRGTQIASPAFDASIWELWPYLA
                     SGASVHIPDEETRLSSPGMIRWWAEQRITLAYLMTPLAEGVLDEEIPPGLELPTRALI
                     IGGDRLHRGPLPGVGFRLMNHYGPAEYSVTSTVVEVPPRGAASGIPTIGRAVDNTRIY
                     LLGRHGEPVGIGVPGELYVAGVGLARGYLRRPQLTAEKFVPDPFATEPGARMYRTGDL
                     VRWLPDGDIDFIGRLDHQVKVRGFRIELGEIETVLAEHPAVTANAVLALQEPAGQVRL
                     VAYVVHDPERVADSGERASLLSEQVADWQALYENTYERGRSAEEASLNLVGWDSSYTR
                     EPIPAGEMLEWAERTVEEILALEPKRVLEIGVGTGLLLYRIAPFVELYRGTDFSHQVI
                     AWLSEQLRESMPQVELARRTAEDFSGVEPRSFDMVILNSVVQYFPDADYLAQVLGGAV
                     EAMADGGTIYVGDVRSLSLLEAFHASVELFKAPSSLPLERLRQIAAAQRLEETELVLD
                     PGFFFALKDHLPRITTVEVWPKHDRADNELTRFRYQVVLRVGGETPPPPTVPWLSWER
                     EEGLSLEELRRRLAENPETLALSGLPNVRVEQAVAAAQILAGAGEGEGPKTAGDLRDR
                     LARLTSSGVHPADLLALARELGYEAVLSWAQAGAQGSFDVLFRRPGSPPTEMPGPAAD
                     GPWSRFANNPVLGKLSRRLMPALRSYLEQRLPAYMVPSTFVLLDGL"
BASE COUNT          558 a         1189 c         1249 g          581 t
ORIGIN      
        1 gctgccggag ctgcccatcc agtacgccga ctttgcggtc tggcagcggc agtggttcga
       61 gggggagctc ttccaggagc agctcagcta ctggaagagc cagctggcag ggctcgtgga
      121 aagccccctg gagctgccca ccgatcaccc acggccgccc atcgagtcat tccgggggga
      181 tctggagtcc tctcatctcg cggctccact cgtccacgag ctgaaagccc tggcccgccg
      241 gcaccgggcg acgctcttca tggtcgtcct gtccggattc aacatcctgc tctcccgcta
      301 ttcggggcag caggacatcg ggatcggcac cggcatcgcc aaccgcaacc ggcgggagat
      361 cgaggatctg atcgggttct tcgtcaatac gttggtcctg cgctccgatc tgtcgggcat
      421 gccgagcttc gagcaattcc tggcgcgggt gcggacggcg acggtggagg cgtacgacca
      481 ccaggacctg ccgttcgaga tgctgatcga gaagatcaat ccggagcggg ccctgaaccg
      541 ccaccctctc gttcaggtga tgctcgcgtt ccaaaacttc cctcgctcgg ccaggaacct
      601 gcccggactg agccttacgg gctcggacga gcggctggcc cagatcagca cggcgaagtt
      661 cgacttggcg ctgttcgtca ccgaacccgg agaggagctt gggctcatac tgcagtacag
      721 caccgatctg ttcgaggtgc acacgatccg ccgcctgctc gctcatctgg agaggctgct
      781 gtgggcagtg gtggagcacc cggaggctgc ggtcacagag ctgcccctgc tgacaggggc
      841 ggagcaggcg caggtgctgc gcgagtggag cgacacgaca aagatctgcc cgcagaggcc
      901 gctggtccac gagctcttcg ccgagcacgc ccggcgccgg ccgcatgccg aggcggtcac
      961 ctcctgggac ggcggacggt tgacctacgg tgaggtcgag gcgcgcgcca accgcctggc
     1021 gcaccatctg cgctcgctcg gcgtggggcc agatgtattg gtgggggtcg cggccgagcg
     1081 aacctgtgag cggatcgtgg ggatcgtggc tgtgctcaag gccggtggcg cctacgtcac
     1141 cctcgacccg acttatccca gggagcggct ggccttcctg ctcgacgacg cccgcgttcc
     1201 ggtgctgctg actgaggagc gcttcgccga cgcgcttccc gactgtacgg cggaggtcat
     1261 ccgcctggat gcggactggg catcgatccg cggcgatgaa gtggccggac ccgcgagcgg
     1321 tgtcggcccg gacaacctgg cctatgtggt ctatacctcg ggctcgaccg gcaaaccgaa
     1381 aggcgtggag atcccccatg ccggcctgat gaacctggtg cgctggcacc gcgatctcta
     1441 ccaggtgaca cccgaagacc gcggcacaca gatcgccagc ccggctttcg atgcttcgat
     1501 ctgggagcta tggccctacc tggcgagcgg cgcctcggtc catattcccg acgaggagac
     1561 ccgcctctcc tcgccgggaa tgatccgctg gtgggccgag caacggatca ccctggccta
     1621 cctgatgacg ccgctcgccg aaggggtgct ggacgaggag atcccccccg ggctcgagct
     1681 gccgacacgg gcgctgatca tcggcggcga tcggctgcac cgcggtcccc tgccgggcgt
     1741 cggcttccgg ctgatgaacc actacggacc ggccgagtac tcggtgacgt cgaccgtggt
     1801 ggaggtgccg ccgcgcggcg cggctagtgg cataccgacc attggacgag cggtcgacaa
     1861 cacgcggatc tacctgctgg gcaggcatgg cgagccggtg gggatcggcg tgccggggga
     1921 gctctatgtg gcgggcgtgg gactcgcccg cggctatctc cggcggccgc agctgacggc
     1981 ggagaagttc gtgcccgatc ccttcgccac cgagccggga gcgcgcatgt accgcaccgg
     2041 cgatctggtg cgctggctgc cggacggcga catcgacttc atcggccggt tggatcatca
     2101 ggtgaaggtc cgcggcttcc gcatcgagct gggggagatc gagaccgtgc tggccgagca
     2161 cccggcggtg acggcgaacg ctgtcctggc cctgcaggaa cccgccgggc aggtgcgcct
     2221 ggtcgcctac gtggtgcacg acccggaaag ggtggcggac tccggagaac gggcctcctt
     2281 gctctctgag caggtggcgg actggcaggc gctctacgaa aacacttacg agcgggggcg
     2341 cagcgcggaa gaggcctctc tcaacctagt gggctgggac agcagctata cacgggagcc
     2401 gatcccggcc ggggagatgt tggagtgggc ggagcgcacg gtcgaggaga tcctggcgct
     2461 ggagccgaag cgggtgctcg aaatcggcgt cggcaccggc ctcctcctct accggattgc
     2521 ccccttcgtc gagctctatc gcggcaccga cttctcgcac caggtgatcg cctggctttc
     2581 cgagcaactc agggagagca tgccgcaggt cgagctggcg cggcgcacgg ccgaggactt
     2641 ctcgggtgtc gagccgcgct cgttcgacat ggtgattctg aactcggtcg tccagtactt
     2701 cccggacgcc gactacctgg cccaggtcct cggcggagcg gtcgaggcga tggccgacgg
     2761 cggcacgatc tacgtgggcg acgtccggag cctgtcgctg ctggaggcct tccacgcttc
     2821 ggtcgagctg ttcaaagccc cctcttccct gccactggaa cggctgcgcc agatcgctgc
     2881 ggcgcaacgg ctggaggaga ccgagctggt gctcgatccg ggcttcttct tcgcgctcaa
     2941 ggatcacctg ccgcgaatca ccaccgtgga ggtgtggccg aagcacgatc gggcggacaa
     3001 cgagctgacc cgcttccgct atcaagtcgt tctgcgggtc ggcggcgaga ccccgccgcc
     3061 gccgaccgtc ccctggctga gctgggagag ggaggagggg ttgagcctcg aggagctacg
     3121 gcggcggctg gcggaaaatc cggagacgct ggcgctctcc ggattgccca acgtgcgtgt
     3181 ggagcaggcc gtcgccgcgg cgcagatcct cgccggagcc ggagagggag aggggccgaa
     3241 aacggctggc gatctgcgcg atcggctggc acggctcacc tcatccggcg tgcaccccgc
     3301 ggacctcctg gccctcgccc gtgagctcgg ctacgaagcc gtcctgagct gggcgcaggc
     3361 gggagcacag gggagcttcg acgtcctctt ccgccgcccc ggatcaccgc cgaccgagat
     3421 gcccgggccc gcagcggacg gaccgtggag ccggttcgcc aacaacccgg tcctggggaa
     3481 gctctcccgc cgcttgatgc cggctctgcg gtcctatctc gagcagcgcc tgcccgccta
     3541 tatggtcccc tcgaccttcg tgctgctcga cgggctg
//
LOCUS       DASPJI010000518        17108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_379411, whole genome shotgun sequence.
ACCESSION   DASPJI010000518 DASPJI010000000
VERSION     DASPJI010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17108)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17108
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379411"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="VEL74_14005"
     CDS             complement(<1..1383)
                     /locus_tag="VEL74_14005"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006106526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYG63690.1"
                     /translation="MQRRLLGALLAILIFFFLNIGVRFWGERSRAESLAELQNAVERQ
                     LLINGIGRHLGDARREVALLAGGFLSEQGPVTDEAREAFAARLRRIHSGIAQVRAIPG
                     SSTPLADRVAADARELTASWARAYESFGVDSGRAIAELSLRSDPLSRQVSELLPRWEL
                     EERRRVARASQELQRVSELTDRVAVLFFVLSTLVMTLVAIHVSHFLVDSNRRLEERVH
                     ERTRALEQEVQERRKAEDQLFHDAFHDPLTDLANRALFLDRLGLSLVRAKRRPAYHFA
                     VLFFDLDRFKLINDSLGHLSGDQTLVTVARRLEACVRPGDTVARLGGDEFAILLDDIR
                     DLSDVDRLTEQVEERLAAPIQLGGNEIFISSSIGIAFGKAAYERPEEILRDADAAMYR
                     AKALGRARHEVFSEELHLAALDRLRLETDLRRAVQEQSFRVQYQPIISLPDGRLTGFE
                     ALVRWWHPAWG"
     gene            complement(1399..1911)
                     /locus_tag="VEL74_14010"
     CDS             complement(1399..1911)
                     /locus_tag="VEL74_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63691.1"
                     /translation="MLRTGLTCGWSVALLTVLVGLLPAEVGARKAKVEEPIAVIVHER
                     VAVDDLSLPELRRIFLGERRAWVSDRMITLLTPPRDSPERKVLLNDILGRRSEDQYQH
                     HWFNKLLDDGTQVTPKITGSPEITAGLVREIPGAIALIRLSRVPRGVKVLRIDGKRPD
                     EAGYPLRTSG"
     gene            2061..2669
                     /locus_tag="VEL74_14015"
     CDS             2061..2669
                     /locus_tag="VEL74_14015"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG63692.1"
                     /translation="MPKREPTPRSAFDAAYYHRFYGDPRTRVADPESCAALAGFVFSY
                     LQYMGVPVERVLDIGCGVGLWRQEVLRRYPAARYVGVEKSDHACREHGWEKGCVTDYR
                     AEERFDLVICQGVLPYLDDAGAEAAIRNLPNLVRSALYLEVLTREDWERNCDQRVTDG
                     RVHLRSAAWYRERLRRDFLACGGGLFLRKGTPVALFELEYLA"
     gene            2706..4445
                     /locus_tag="VEL74_14020"
     CDS             2706..4445
                     /locus_tag="VEL74_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63693.1"
                     /translation="MSTPRDPETGGGPRSRRLPVWPVAFAVYLFHALLFGGWIVDDAG
                     ISFVYARNLAQGHGLVSQPGLAPVEGYSNFLWVLLLTPPLLLHLFHPVWTPKLLSLGL
                     AAGTFFLLDRSLTRLSGGSRGLSWMTLLLLAVCTPFVIWTVSGLENPLTVFLAVALFW
                     ILVTERREEAPLPSSAGRWALLAGAVAAGLAMTRPDGVVYAALYPLLTPRWGRQSVLT
                     SLRSGAGRAALSIGVFALLFGAFLGFRVLYFGDLYPNTYYAKGSPSPVALLPLLTFQP
                     PAVAKLFTLMYGFAGTWGGLLLVALGAATAFLAGRRRFHWGHAALLAFSGLAVFVYIV
                     LPHDWMPEYRFATPLFPFFYSYVVLLAASLGGELFAEPSRRHLAALAGGILAVGLSLV
                     SFASRSVLFAAAPTVPFAETVEDYGLRYNRYADLLGVREGSLLVPDVGGTLWASRLRI
                     YDLGALCDRTIARTRGVDQQAFYDYVFAQARPTFIHSHNYWTVVSALELDPRFRRDYV
                     PIFEYDEPHGRQRLGHDYHSGDWVRREVAQGREAELLAIGADLEEQYRRRLASRAREA
                     SAPDGPLDPLSPP"
     gene            4453..5175
                     /locus_tag="VEL74_14025"
     CDS             4453..5175
                     /locus_tag="VEL74_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63694.1"
                     /translation="MGGNLWLDLLAALLPASERGGLIRRGAAPERWSLLLGLVELFVG
                     TTWLISNALDSFQGMSDATATYLMEQVDPAQFEQAKVVITSGGPLIWLTWAVRPTTWL
                     LASIPATGVARLVAFAVSRETVGEPLVWLALRPFQGAGHLLRLLRKRLRFGPVRPDRI
                     VLEPGYDLAVLSCRPKPDWNERVTIEIAGRFYWLRHCEERQDGGWWVHAHCLEEAGPH
                     EVFRGLVRYEPPATARRLLARR"
     gene            5255..5473
                     /locus_tag="VEL74_14030"
     CDS             5255..5473
                     /locus_tag="VEL74_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63695.1"
                     /translation="MKKTLRCFALAGVIGMSAWLSFPATAHAFISCEEYEDERFCYDP
                     SGTHWLCTWASTGTPGLCVCDFGRWFCW"
     gene            5653..6078
                     /locus_tag="VEL74_14035"
     CDS             5653..6078
                     /locus_tag="VEL74_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HYG63696.1"
                     /translation="MSTAKTHEILLREAGLRVTRPRVAVLDAVRRHPHTDTESIIAAV
                     RADLGEVSHQAVYDVLRALTTAGLLRRIQPAGTQARYEARVGDNHHHVICRSCGVIAD
                     VDCATGEAPCLTASDDHGFRIDEAEVIYWGLCPDCSKAP"
     gene            6117..8300
                     /gene="katG"
                     /locus_tag="VEL74_14040"
     CDS             6117..8300
                     /gene="katG"
                     /locus_tag="VEL74_14040"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HYG63697.1"
                     /translation="MSVSESENPAIPAPTPKAHRPRSNKDWWPNQLDLNVLHQHHPRG
                     NPLGDGFDYAAAFAGVDVEELKRDIIQVMTTSQDWWPADFGHYGPLFIRMSWHAAGTY
                     RIEDGRGGGGDGAQRFAPLNSWPDNANLDKARRLLWPVKKKYGQSVSWADLLVFAGNC
                     AMESMGFKTFGFGFGREDIWEPEEIFWGPEDAWLGDERYSGDRELAGALGAVQMGLIY
                     VNPEGPNGKPDPLAAARDIRETFRRMAMNDEETVALIAGGHSFGKTHGAASAKHVGPE
                     PEGCPIHSMGLGWKNSYRTGKGPDTITSGLEVTWTKTPTQWSNNFFENLFAHEWELVE
                     SPAGAKQWVAKDGEEIIPDPFDPAKKHRPTMLTTDLALRMDPSYGKISRRFLENPDEF
                     ALAFAKAWYKLLHRDMGPVSRYLGPWVPEPQIWQDPVPAVDHPLVDAADVAALKEKLL
                     ASGLTVQQLVLTAWASAASFRGTDMRGGANGARIRLAPQRDWEVNEPAELATVLATLE
                     EIREEFNGTQPGGKKISMADLIVLGGCAGVKKAAKDAGFDVGVPFTPGRTDATQEQTD
                     ADSFTVLEPRADGFRNYVRAGQSLSPETLLLDRANMLTLSAPEMTVLVGGMRALGANT
                     GRTRHGVFTDRPGVLTNDFFVKLLEPGTDWQESGEDHVYVGRDQATGETKWTATAVDL
                     IFGTNSQLRAIAEVYASDDAKRKFVDDFVAAWVKVMDLDRFDVRR"
     gene            8423..8647
                     /locus_tag="VEL74_14045"
     CDS             8423..8647
                     /locus_tag="VEL74_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63698.1"
                     /translation="MKKTLRCLGLAGILGLTLWVSQPQPAHALPACAFLQRQPCWPLN
                     SKVACSWEEEGLSGTCHCMLQGWICVIQFS"
     gene            8693..8926
                     /locus_tag="VEL74_14050"
     CDS             8693..8926
                     /locus_tag="VEL74_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63699.1"
                     /translation="MKKTLRCLGLAGILGLTLWVSQPQPAHALPTCESLQFQDCRPFN
                     STTPCVWASSGSQSTCWCYFLRGWICLQSLDQT"
     gene            complement(9021..9575)
                     /locus_tag="VEL74_14055"
     CDS             complement(9021..9575)
                     /locus_tag="VEL74_14055"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HYG63700.1"
                     /translation="MALHDRSVRLTYDDYLLFPEDGLRHEIIDGEHWVSAAPFLKHQD
                     VLLALATLLRAFVKERGLGRVYVAPVDVLLSRHDVVQPDLVFVSRERAAILTERNIQG
                     APDLVAEILSDGTRKVDEEVKLELFERFGIREYWLVDPRRERVRVYRLRQEAFELTGE
                     LTARETLTTPLLPGMTVPLADVFR"
     gene            9855..10049
                     /locus_tag="VEL74_14060"
     CDS             9855..10049
                     /locus_tag="VEL74_14060"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HYG63701.1"
                     /translation="MTSCGRSWAVHLYEQGRLSVGKAHELAGLSLWEFRQILGSRRIA
                     PHLDAEDLEQDLETLRELRG"
     gene            10049..10423
                     /locus_tag="VEL74_14065"
     CDS             10049..10423
                     /locus_tag="VEL74_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HYG63702.1"
                     /translation="MIVVSNTSPLTNLAAIHQFGLLQKLFGEIRIAEGVWRELNAGSH
                     RHPGSREVERAAWIRRHAVGNKALVTALRRDLDLGEAETLVLALELRANLVLVDEQEG
                     RNMASQLGLRPESYLAGDKLQP"
     gene            complement(10783..12693)
                     /locus_tag="VEL74_14070"
     CDS             complement(10783..12693)
                     /locus_tag="VEL74_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63703.1"
                     /translation="MKSKLRWICPALLLAMLAPGASALAQPDDRSAEDLLASMVREGW
                     KPVVTGVVQRQGEGGRVETFAIGPAASRRAIQELESRLELLSREYRAYPKEDIRRGIQ
                     SLQQKLSRLREDLKASRDEPLSSVMDKCMVNRGASADAYPLTPAGAGATASAYFNSDC
                     AYEAETYASAYARAAANGNVDIVVQESPRTGTNISSNATASVQGTSNCLAEAYSYHRY
                     TPANIFHSVFDSDTNCTDTTPPPVTTVGIGRIDDVPAATLLLPYFEVDLDDANGVTTL
                     FSVNNASDKAVLAHVTLWTDQSVPAIDYDIYLTGFDVQTINLRDIFVNGNLSITASAG
                     QDPADTISNKGVASQDINFASCTGVLPYANPAVSASFRAHLRAWFTGNQSPATGNCAG
                     ARIRDGIARGYVTIDTVNACNLLFPSSPGYTALLTAQNVLWGDYSYVHSGQNFAQSET
                     LVHIEACSTCFGAGDHTFYGRYGGGDFREPLPTTLAARYTNGGAFNGGTDFLVWREGN
                     QAAAGYSCSAAGPAAWYPLDAAQIVVFDEEENPVQQNTTCPAGAPNCVRIPNEAQRVN
                     VAEDLLAPFDFGWVYLNLQHSKLTPYQDPYAQAWLMTVSNAEGRFSTGHNGIQLDNAN
                     RPITTRIPVSVP"
     gene            13080..13568
                     /locus_tag="VEL74_14075"
     CDS             13080..13568
                     /locus_tag="VEL74_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63704.1"
                     /translation="MSEGPHHPNAFTARFLARLGERDEPLTAGEAAVAGPWRIEAIPG
                     RGFGLFRVGQSLVRGFRPAAVCTSRWVALAFAAMLPGTGRDPLLRLGKEADAEGLYPV
                     TLDTGEVVAGLEVFDDTLIEHVNALIHLLQAPLSLANALDAAGAEALGSCGSILDERA
                     HV"
     gene            complement(14382..16181)
                     /gene="lepA"
                     /locus_tag="VEL74_14080"
     CDS             complement(14382..16181)
                     /gene="lepA"
                     /locus_tag="VEL74_14080"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HYG63705.1"
                     /translation="MAPHERIRNFSIIAHIDHGKSTLADRILQRTGAVADRDMMDQLL
                     DDMDLERERGITIKARAVRLFYKARDGETYQLNLIDTPGHVDFAYEVSRSLAACEGAV
                     LVVDAAQGVEAQTLANAYLAVNANLAIVPVINKIDLPAADPDRVKEQIENVIGIDAHD
                     AVLASAKAGLGIDDVLERVVTDIPAPQGDPEAPLKALLFDSWYDPYRGVACMVRIVDG
                     TLRKGEKIQFMSSRRAYEVDEVGIFNPKPGTVESLSVGEAGFFYAGIKDLLLAQIGDT
                     VTHAARPAAAAYPGFQEVKPMVFAGLYPVSADDYEDLRDAVEKLRLNDASFSFEPESS
                     GALGFGFRCGFLGLLHMEIIQARLEREFNLDLITTAPGVRYRAHTTDGDCVEVDSPSK
                     LPAPGRLDYLEEPYIQATIITRSEYVGGILALAEERRGEQRSLNFMTTDRVLIEYDFP
                     LAEVIHDFYDRLKSVSRGYASFDYSMSGYRRSELVKLDILVNGDPVDALSLIVHRDKA
                     YARGKVLVEKMKELIPRQLFEVVLQAAIGARVIARETVRAISKNVIAKCYGGDITRKR
                     KLIKKQKEGKKRMKRVGRVDIPQEAFLAVLRVD"
     gene            complement(16301..>17108)
                     /locus_tag="VEL74_14085"
     CDS             complement(16301..>17108)
                     /locus_tag="VEL74_14085"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HYG63706.1"
                     /translation="VRFVLSGLILYGMARAGGVPRPKPAQWWPATVVGVLLLAVGNGG
                     VVWAEHRVDSGLAALMVATEPLWIVLLLWLAPGGAKPGPRVLAGLGLGLAGLVFLIRP
                     GGGGIDPVGAVVLAAASFSWAWGSLYARRAALPESPILTSGMQMLMGGVVLLAVSAIT
                     GEPGRIDVGQISLRSLLALGYLVIGGLIAFTCYSWLMRNARPVLASTYAYVNPVVAVF
                     LGWAFAGEPLTAGMLGAAAVIVTGVALISTAQGSSPEPGAEDLEEERAAA"
BASE COUNT         2678 a         5866 c         5787 g         2777 t
ORIGIN      
        1 gccccaggcc ggatgccacc agcgcaccag cgcctcgaag ccggtcaagc ggccgtccgg
       61 cagcgagatg atcggctggt actggacccg gaagctctgc tcctgcacgg cgcgccgcag
      121 gtcggtctcg aggcgcagcc ggtcgagggc cgcgagatgg agctcctcgc tgaagacctc
      181 gtgccgggcc cggccgagcg ccttcgcccg gtacatcgcg gcgtcggcgt cgcgcaggat
      241 ctcctccggc cgctcgtagg ccgccttgcc gaaggcgatg ccgatgctgg agctgatgaa
      301 gatctcgttg ccgccgagct ggatcggcgc ggccagccgc tcctccacct gctccgtcag
      361 ccggtcgacg tccgacaggt cgcggatgtc gtcgagcagg atcgcgaact cgtcgccgcc
      421 gagccgcgcg acggtgtcgc ccggccgcac gcaggcctcc agccggcggg cgacggtgac
      481 cagcgtctgg tcgccgctca ggtggccgag gctgtcgttg atcagcttga aacggtccaa
      541 gtcgaagaac agcaccgcga agtggtacgc cggccgccgc ttcgcccgta ccagcgacag
      601 ccccagccgg tcgaggaaga gggcgcggtt ggcgaggtcg gtcagcgggt cgtggaaggc
      661 gtcgtggaag agctggtcct cggccttgcg gcgctcctgg acctcctgct ccagcgcccg
      721 cgtccgctcg tgcacccgct cctccaggcg ccggttcgag tcgaccagga aatgggagac
      781 gtggatggcg accagggtca tgaccagggt cgacagcacg aagaagagga ccgccacccg
      841 gtcggtcagc tcggagaccc gctgcagctc ctggctcgcc cgtgccaccc gccgccgctc
      901 ctccagctcc catcgcggca ggagctccga cacctggcgg ctcagggggt ccgagcgtag
      961 cgacagctcg gcgatcgccc tcccgctgtc cacgccgaag ctctcgtagg cccgcgccca
     1021 ggacgcggtg agctcccggg cgtccgcggc gacccggtcg gcgagcggcg tggagctgcc
     1081 ggggatcgcc cgcacctggg cgatcccgga gtggatccgg cgcaggcggg cggcgaaggc
     1141 ctcccgcgcc tcgtcggtca ccggcccctg ctcgctgagg aatcctccgg ccaaaagcgc
     1201 gacttcccgg cgggcgtctc ccagatgccg gccgatgccg ttgatcagga gctgccgctc
     1261 gaccgcgttc tgcagctccg cgaggctctc ggcccgcgag cgctcgcccc agaaccgcac
     1321 tccgatgttg aggaagaaga agatcaggat cgccagcagg gccccgagca agcgtctctg
     1381 catggccatc ggcaccggtc agcccgaggt cctcagcggg taaccggcct cgtcggggcg
     1441 tttgccgtcg atccgcaaga ccttcacacc ccggggcacc cggctcagcc ggatcagggc
     1501 gatggcgccc gggatctcgc ggacgaggcc cgccgtgatc tcgggagagc cggtgatctt
     1561 gggggtgacc tgcgtgccgt cgtcgagcag cttgttgaac cagtggtgct ggtactggtc
     1621 ctcgctccgg cggcccagga tgtcgttcag gaggactttc cgttccgggg agtcgcgtgg
     1681 cggggtcagc agggtgatca tccggtcgct cacccaggcc cggcgttcac ccaggaagat
     1741 ccgccgcagc tcggggaggc tcaggtcgtc gaccgccacc cgctcgtgaa cgatgaccgc
     1801 gatcggctcc tcgactttgg ccttgcgggc gccgacctct gccgggagga gcccgacgag
     1861 gacggtgagg agcgccacgc tccagccgca ggtaagtccg gtccgcagca tcgttcgacc
     1921 caatcaagca aagatccacc agatccggcc agggggccgg gcccgactac gtgtccttta
     1981 acggtcctgc aagatcgggg ccgccagccc accagggtgc ccgatgacag ggggcgccgg
     2041 ttcgggcagg atatccggcg atgccgaagc gagagcccac cccgcgcagc gccttcgacg
     2101 cggcctacta ccaccggttc tatggcgatc cgcgtacgcg ggtggccgac ccggagagct
     2161 gcgcggcgct ggcgggcttc gtcttctcct atctccagta catgggggtt cccgtcgagc
     2221 gggtgctgga tatcggctgc ggcgtcgggc tgtggcggca ggaggtgctc cggcgttacc
     2281 ccgcggcgcg ctacgtcggc gtcgagaaga gcgaccacgc ctgccgcgag cacggctggg
     2341 agaaggggtg cgtcaccgac taccgggcgg aggagcgttt cgacctggtc atctgccagg
     2401 gggtgctgcc gtatctcgac gacgccgggg cggaggcggc gatccgcaac ctccccaacc
     2461 tcgtccggtc ggccctctac ctcgaggtcc tcacccggga ggactgggaa cgcaactgcg
     2521 accagcgggt gaccgacggc agggtccacc tgcggagcgc cgcctggtac cgggagcggc
     2581 tgcggcgcga cttcctggcc tgcgggggcg gcctcttcct gcgcaagggc acgccggtgg
     2641 cgctcttcga gctggagtac ctggcgtaga atgtcgcgtc agccaaagcg agagagctac
     2701 agcgagtgag caccccacga gatcccgaga cgggaggagg cccgcgcagc cggcggctgc
     2761 cggtctggcc cgtcgccttc gccgtctacc tcttccatgc cctgctcttc ggggggtgga
     2821 tcgtggacga cgcgggaatc tcgttcgtct atgcccgcaa cctcgcccag ggccatggtc
     2881 tggtctccca gccggggctg gcgccggtcg aaggctactc gaacttcctc tgggtgctgc
     2941 tgctgacccc gcccctgctc ctgcacctct tccacccggt ctggacgccc aagctgctga
     3001 gcctgggtct ggcggcgggc accttcttcc tgctcgaccg ctccctgacc cgcctctccg
     3061 gggggagccg cggcctctcc tggatgacgc tgctgctgct cgcggtgtgc acgccattcg
     3121 tgatctggac ggtatccggg ctggagaacc cgctcaccgt cttcctcgcc gtcgcgctgt
     3181 tctggatcct ggtgacggag cgccgggagg aggcgccgtt gccttcatcg gcggggcgct
     3241 gggcgctgct ggcgggagcc gtggcggccg ggctcgcgat gacccggccc gacggggtcg
     3301 tctatgccgc cctctatccg ctgctcactc cgcgatgggg caggcagagc gtcctgacat
     3361 ccctgcggtc cggcgccggc cgcgccgccc tctcgatcgg cgtcttcgcc ctgctcttcg
     3421 gcgccttcct gggcttccgg gtgctctact tcggggacct ctacccgaat acctactacg
     3481 ccaagggcag cccttcaccg gtggccctgc tgccgctgct gaccttccag ccgccggccg
     3541 tggccaagct gttcaccctg atgtacggct tcgccggcac ctggggcggg ctgctgctcg
     3601 tggcgctcgg ggccgcgacg gcgttcctcg ccggccgccg gcgcttccat tggggacacg
     3661 ccgccctgct ggccttctcc ggcctggccg tgttcgtcta catcgtcctc ccccacgact
     3721 ggatgccgga gtatcgcttc gccacgccgc tcttcccttt cttctattcc tacgtcgtgc
     3781 tgctggccgc gagcctgggc ggggagctct tcgccgagcc ctcacggcgg caccttgcgg
     3841 cgctggcggg cggcatcctg gccgtggggc tcagcctggt ctccttcgcc agccgttccg
     3901 ttctcttcgc cgcggcgccc accgtcccct tcgccgagac ggtggaggac tacggcctgc
     3961 gctacaaccg gtacgccgac ctgctggggg tgagggaggg ctcgctcctg gttcccgacg
     4021 tgggcgggac gctctgggcc tcgcgcctgc ggatctacga cctcggggcc ctgtgcgacc
     4081 gcaccatcgc ccggactcgg ggcgtggacc agcaggcctt ctacgactac gtcttcgcgc
     4141 aggcgaggcc gacgttcatc cacagccaca actactggac ggtggtctcg gcgctggagc
     4201 tggacccgcg cttccggcgt gactacgtgc ccatcttcga gtacgacgag ccgcatggcc
     4261 ggcagcggct cggccacgat taccactccg gcgactgggt gcggcgagag gtggctcagg
     4321 ggcgcgaggc ggagctcctg gcgatcggcg cggacctcga agagcagtac cggcggcgcc
     4381 tcgccagccg ggcccgggag gcctccgcgc cggacggccc gctggatccg ctctcacccc
     4441 cttgacgaga tcatgggcgg caacctctgg ctggacctgc tggcggccct cctgcccgcg
     4501 tccgagcggg gcggcctcat ccgccgcggc gccgccccgg agaggtggtc gctgctcctc
     4561 ggcctggtgg agctcttcgt cggcactacg tggctcatct cgaacgccct cgattcgttc
     4621 caggggatgt ccgacgcaac cgccacctat ctgatggaac aggtggaccc ggcgcagttc
     4681 gagcaggcca aggtggtcat cacctcgggc ggccctctca tctggctgac ctgggcggtc
     4741 cggcccacca cctggctcct cgccagcatc ccggccacgg gggtggcgcg cctggtggcg
     4801 ttcgccgtca gccgcgagac ggtgggggag ccgctggtct ggctggccct gcggccgttc
     4861 cagggggccg gccatctcct ccggctcctc cgcaagcgcc tccgtttcgg ccccgtgcgg
     4921 cccgaccgga tcgtgctcga gccgggctat gacctggcgg tgctgagctg ccggccgaag
     4981 cccgactgga acgagagggt gacgatcgag atcgccggcc gtttctattg gctgcggcac
     5041 tgcgaggaga ggcaggacgg gggctggtgg gtccatgccc actgcctgga ggaggccggt
     5101 ccgcacgagg tcttccgcgg cctcgtccgc tacgagccac ccgccacggc ccgccggctc
     5161 ctggcgcgcc gctgacgcac catcgccgcc gcactcaccc tccttccatg caggcattgc
     5221 taatgctatg cttcaagaaa ggaggattca cctcatgaag aagacgcttc gctgctttgc
     5281 gttggcggga gtgatcggta tgtcggcctg gctgtctttc cctgccacgg cccatgcctt
     5341 catctcctgc gaggagtacg aggacgagcg cttttgctat gacccgagcg gcacccactg
     5401 gctctgtacg tgggcctcta cggggactcc cgggctctgc gtctgcgatt tcggccgctg
     5461 gttctgctgg taagaggcgg tctcacgctc ccatgaaccg acgggaggct ctccgcagcc
     5521 tcccgtcgct cgcacccctc ccgcggccac ccgccgatga agagcctttg agaggacccc
     5581 cgcctggact ggatgtaggg gaggggcagg cctaatcttg aacgattcca gaaaatccgt
     5641 catgctcccc gcatgtccac ggccaagacc cacgagatcc tcctgaggga ggcggggctg
     5701 cgggtgacgc ggccccgggt ggccgtgctc gacgcggtgc ggcggcaccc gcacaccgac
     5761 accgagtcga tcatcgcggc ggtccgggcc gatctcggcg aggtctccca ccaggccgtg
     5821 tacgacgtcc tgcgcgccct gacgacggcg ggcctgctcc ggcggatcca gccggcgggg
     5881 acccaggccc ggtacgaggc gcgggtgggc gacaaccacc accacgtcat ctgccggtcg
     5941 tgcggggtca tcgccgacgt cgactgcgcg acgggcgagg cgccgtgcct gaccgcgtcc
     6001 gacgaccacg gcttcaggat cgacgaggcc gaggtcatct actggggcct ttgtcccgac
     6061 tgcagcaagg ctccgtgaac ccaacaagat tcagtgagac aggagaagga tcgaccgtga
     6121 gcgtgtccga gagcgaaaat ccagcaatcc cagcaccgac cccgaaggcg caccggccgc
     6181 gcagcaacaa ggactggtgg ccgaaccagc tcgacctcaa tgtcctgcac cagcaccacc
     6241 cgcggggcaa cccgctgggc gacggcttcg actacgccgc ggcgttcgcc ggcgtggacg
     6301 tcgaggagct caagcgcgac atcatccagg tcatgacgac ctcgcaggac tggtggcccg
     6361 ccgacttcgg ccactacggc ccgctgttca tccggatgag ctggcacgcc gccggcacct
     6421 accgcatcga ggacggccgc ggcggcggcg gtgacggcgc ccagcgcttc gcccccctca
     6481 acagctggcc cgacaacgcc aacctcgaca aggcccgccg tctgctctgg ccggtcaaga
     6541 agaagtacgg ccagagcgtc tcgtgggccg acctgctggt cttcgccggc aactgcgcca
     6601 tggagtcgat gggcttcaag accttcggct tcggcttcgg ccgcgaggac atctgggagc
     6661 ccgaggagat cttctggggc cccgaggacg cctggctcgg cgacgagcgc tacagcggcg
     6721 accgcgagct cgccggtgcg ctcggcgcgg tccagatggg gctgatctac gtcaaccccg
     6781 agggccccaa cggcaagccc gacccgctgg ccgccgcgcg cgacatccgc gagaccttcc
     6841 gccggatggc gatgaacgac gaggagaccg tcgcgctgat cgccggtggc cactccttcg
     6901 gcaagaccca cggcgcggcc tcggccaagc acgtcggccc cgagccggag ggctgcccca
     6961 tccacagcat ggggttgggc tggaagaact cctaccgcac cggcaagggc ccggacacca
     7021 tcacctccgg cctcgaggtc acctggacca agacgccgac gcagtggagc aacaacttct
     7081 tcgagaacct cttcgcccac gagtgggagc tcgtggagag ccccgcgggc gccaagcagt
     7141 gggtggccaa ggacggcgag gagatcatcc ccgacccgtt cgacccggcg aagaagcacc
     7201 gcccgaccat gctgacgacc gacctcgcgc tccgcatgga cccgtcgtac gggaagatct
     7261 cgcgccgctt cctggagaac ccggacgagt tcgcgctcgc cttcgccaag gcctggtaca
     7321 agctgctgca ccgtgacatg ggcccggtct cccgctacct cggcccctgg gtccccgagc
     7381 cccagatctg gcaggacccc gtaccggccg tcgaccaccc gctggtcgac gcggcggacg
     7441 tcgccgcgct caaggagaag ctgctcgcct ccggcctgac cgtccagcaa ctggtgctca
     7501 ccgcctgggc ctcggccgcc agcttccgcg gcaccgacat gcgcggcggc gcgaacggcg
     7561 cccgtatccg gctggccccg caacgcgact gggaggtcaa cgagccggcc gagctggcca
     7621 cggtgctcgc gaccctcgag gagatccggg aggagttcaa cggcacccag ccgggcggca
     7681 agaagatctc gatggccgac ctgatcgtgc tcggcggctg cgccggagtg aagaaggcgg
     7741 cgaaggatgc cggcttcgac gtcggcgtgc cgttcacgcc gggccgcacc gacgccacgc
     7801 aggagcagac ggacgccgac tccttcaccg tcctcgagcc gcgggccgac gggttccgca
     7861 actacgtgcg cgcgggccag tcgctgtcgc cggagacgct gctgctcgac cgggcgaaca
     7921 tgctcaccct ctccgcgccg gagatgaccg tcctagtcgg cggcatgcgg gcgctcggcg
     7981 ccaacaccgg ccgcacccgt catggcgtct tcaccgaccg tccgggcgtg ctgaccaacg
     8041 acttcttcgt gaagctgctg gagccgggca cggactggca ggagtccggg gaggatcacg
     8101 tctacgtagg gcgcgaccag gccaccggcg agacgaagtg gaccgccacc gcggtcgacc
     8161 tcatcttcgg caccaactcc cagctccgtg ccatcgccga ggtctacgcc agcgacgacg
     8221 cgaagaggaa gttcgtcgac gacttcgtcg ccgcctgggt caaggtcatg gacctcgacc
     8281 ggttcgacgt ccgccgctga cccccctgcc ccaaccgcca ttctgacccg ggtcctgccg
     8341 gacccgggtc agagcaggcc ggctccttcc cccgcgctgg cgcaccttgt cttcatgagg
     8401 tctctataag gaggttcgac ccttgaagaa aacgcttcgc tgtctcggtc tggccggtat
     8461 cctcgggctc accctctggg tgtcgcagcc ccagcccgct cacgcccttc ccgcctgcgc
     8521 gttcctccag cggcagccct gctggccgct gaactccaag gtcgcctgca gttgggagga
     8581 ggaggggctc tcgggtacct gtcactgcat gctccagggc tggatctgcg tgatccagtt
     8641 ctcctgaggg tgcacgcctc tgacaccttc agatttcaag gagattccac tcatgaagaa
     8701 aacgcttcgc tgtctcggtc tggccggtat cctcgggctc accctctggg tgtcgcagcc
     8761 ccagccggct cacgcccttc ccacctgcga gtctctccag tttcaggact gccggccctt
     8821 caactcgacg accccctgcg tctgggcgtc cagcgggtcg cagtcgacct gctggtgcta
     8881 tttcctgcgc ggctggatct gcctccagtc gctggaccag acgtaggccg tcgcgccagg
     8941 tgtcgcatcc agcccggaga ggtggtcaca ggctgtgacc acctctcctc ctgagccctg
     9001 aagggagggg aggagcagga ctaccggaag acgtcggcga gaggcacggt cattcctggg
     9061 aggagcggcg tggtgagcgt ttcccgggcc gtgagctccc cggtgagctc gaaggcctcc
     9121 tgtcgcagcc tgtacacccg cacgcgttcg cgccgcgggt cgaccagcca gtactcccgt
     9181 atgccgaagc gctcgaacag ctccagcttg acctcctcgt ccaccttgcg ggtgccgtcg
     9241 gacagaatct cggccacgag gtccggggcc ccctggatgt tcctctccgt caggatggcc
     9301 gcccgctccc tggagacgaa gaccagatcg ggctggacga cgtcgtgccg ggagaggagg
     9361 acatcgacgg gtgccacata gactcggccg aggccgcgct ccttgacgaa cgcccggagc
     9421 agagtcgcca gggcgaggag gacatcctga tgtttgagga aaggcgccgc cgacacccaa
     9481 tgctctccgt cgatgatctc gtgccgcagc ccgtcctcgg ggaagaggag gtagtcgtca
     9541 taggtgagcc ggacggagcg gtcgtggaga gccatggcgt gcctcctgtg gagccaactc
     9601 atgctaccac gcctcccggc gttccgccct ccggcgcttc ggacctgaag ccgggaacat
     9661 agcacaccca tccgagagaa accggccctc gagagaggcc tggaggaagg gctcctcccc
     9721 gaagagctca tccaagtaca attgatgagg acttgagagg cccggatcaa ggggcctccg
     9781 gggaggaatt ccagtgcctg cagttcccta tcttgagatc cctcaagacg tgctggactc
     9841 ggctcgtctg acgattgacc agttgcggac ggagctgggc ggtccatctc tacgaacagg
     9901 gacgtctctc ggttggcaag gcccatgagc tggccggtct ctccctctgg gaattccgcc
     9961 agatcctggg atctcgccgt atcgctcccc atctcgacgc cgaggacctg gagcaagacc
    10021 tcgagacgct acgtgagctt cgcggctgat gatcgtcgtc agcaacacct ctcccctgac
    10081 aaacctcgcc gccatccatc agttcgggtt gctgcagaag ctcttcggag aaatccgtat
    10141 cgccgaaggc gtatggcgag agctcaacgc aggaagccac cgccaccccg gcagccggga
    10201 ggtcgagaga gcggcgtgga tccgccgcca cgcggttggc aacaaagccc tcgtgacggc
    10261 actacggcga gacctcgact tgggtgaagc cgaaacgctg gtgctggctc tcgagctcag
    10321 ggcgaatctg gttctcgtcg acgagcagga aggtcgcaac atggcctccc agctcggtct
    10381 ccgaccggag agttatctcg ccggagataa gcttcagccg tgatcagcga agcctggcgc
    10441 gctcgacaca agcttagcgg ggccattcca agcttatccg ggctgactta gcgttacttg
    10501 acgcggggta agcttggcgg gctcgagctg agcttatctg gaaccagcca agcttaacgg
    10561 agtcgggata acgctctccg cggcgaggta agcttcgcgg atctgagata agcgtagatc
    10621 ggacgagata gcgttatctg ggccaggcta agctcagcgg gagcccgtta agcttagctg
    10681 gggcgagcta acgctatttg aggcgggcta agcttagcgg gaccgacgga agcttagctg
    10741 agcccgggcg gggcggcgcc gcccacccgg gctcaggacc gttcaaggca ccgagaccgg
    10801 gatccttgtg gtgatcggcc ggttggcgtt gtcgagttgg atgccgttat ggccggtgct
    10861 gaaccgacct tcggcattac tgaccgtcat cagccaggct tgcgcgtaag ggtcctggta
    10921 gggggtcaac ttggagtgct ggaggttcag gtacacccag ccgaagtcga acggtgcgag
    10981 gaggtcctcg gccacgttga cgcgctgcgc ctcgttcggg atcctgacac agttcggagc
    11041 acccgctggg caggtggtgt tctgctgcac cggattctcc tcctcgtcga acaccacgat
    11101 ttgagccgca tccagcggat accacgccgc cgggccagcc gccgagcacg agtaaccggc
    11161 cgctgcctgg ttgccctcgc gccacaccag gaagtccgtg ccgccgttga acgcgccgcc
    11221 gttggtgtaa cgggctgcca gcgtcgtcgg caggggctcg cggaaatcgc ctccaccgta
    11281 gcggccatag aaggtgtggt cacccgcccc gaagcaggtc gagcaggcct cgatgtgcac
    11341 cagcgtctcg ctctgggcga agttctggcc cgagtggacg taggagtagt cgccccacag
    11401 cacgttctgc gccgtcagca gggcggtgta gcccggcgat gacgggaaca gcaggttgca
    11461 ggcgttgacc gtgtcaattg tcacgtagcc gcgcgcgata ccgtcgcgga tccgggcgcc
    11521 ggcacagttg ccggtggccg gcgactggtt gccggtgaac caggctcgga ggtgggcacg
    11581 gaacgaagcg ctgaccgccg ggttggcgta gggcagcaca cccgtgcacg aagcgaagtt
    11641 gatgtcctgc gacgccactc ccttgttgct gatggtgtcg gccggatcct ggccggccga
    11701 cgctgtgatc gacaggttgc cgttgacgaa gatgtcgcgc aggttgatcg tctgcacgtc
    11761 aaagcccgtc aggtagatgt cgtagtcgat ggccgggacc gactgatcgg tccacagggt
    11821 gacgtgggcc agcacggcct tgtctgaagc gttgttgacg gagaacagcg tggtgacccc
    11881 gttcgcgtcg tcgaggtcga cctcgaaata gggcaagagc aaagtcgccg cgggcacgtc
    11941 gtcgatcctt ccgattccaa cggtggtgac cggggggggc gtcgtgtccg tgcagttcgt
    12001 gtccgaatcg aagaccgagt ggaagatgtt cgccggggtg tagcgatggt acgagtacgc
    12061 ctccgccagg cagttggacg tgccctggac gctcgccgtg gcgttgctcg agatgttggt
    12121 accggtccgc ggagactcct ggacgacgat gtcgacgttg ccgttggcgg ccgcccgggc
    12181 gtaggcggaa gcgtaggtct ccgcctcata ggcgcagtca ctgttgaagt aggccgaagc
    12241 ggtggcgccg gcgccggccg gggtcagggg gtaggcatcg gcgctggctc cgcgattgac
    12301 catgcacttg tccatgacac tcgacagcgg ctcgtcccga gatgccttca gatcctcccg
    12361 aagtctggag agcttctgct gcagagactg gattccccgc cgaatatcct ccttcgggta
    12421 ggcccggtac tcccggctca ggagctccag ccggctctcg agctcttgga tagcccgtct
    12481 cgatgccgcg ggaccgatgg cgaacgtctc gacgcgaccg ccctccccct ggcgctgcac
    12541 aacgccggtg accacgggct tccagccctc ccgtaccatc gaggccagaa ggtcctcagc
    12601 gctccggtca tccggctgtg cgagagccga tgcgcccgga gcgagcatcg cgaggagaag
    12661 ggcagggcag atccagcgga gctttgactt catgaaacct ccttactgca cgaaatcgaa
    12721 ccgacccgct ggccacagcg gccaagcgag tcgatggata gtacataagg agagactaaa
    12781 aacagaaggt gcggagccca ggacgaggcc ttcgagttcc tcatcgtccc gcacggcaac
    12841 ctgttgactt ccgcctagat ctgcttctac aatctccgca tcaagtcgta gcaggacacg
    12901 cccggagagc gcgcggctcc gcgaaagctt ccaggcgtgg gattcaaaac gtcgtacggc
    12961 aggacgtggc ccggcaggga gccgagtcgg cccagaagtg agctggtaac tcacgaccgg
    13021 gcctggccac gacacaagga ctggagttcc ttatgcatgg ttgtgacgat ggtaccagtg
    13081 tgtccgaagg cccccaccat cccaacgcgt tcaccgctcg ttttctggcc cggttgggcg
    13141 agagggacga gccgctcacg gccggtgagg ccgcggtggc gggtccgtgg cggatcgagg
    13201 cgatccccgg caggggcttc ggcctcttcc gggtcggcca gagtctcgtc cgcggctttc
    13261 ggccagcggc ggtctgcacc tcccgctggg tggccctggc gttcgcggcg atgctccccg
    13321 gcacggggcg ggatcccctg ctccgcctcg gcaaggaggc ggacgcggag ggtctctatc
    13381 ccgtgacgct ggacaccggc gaggtcgtgg cgggtctcga ggtcttcgac gacaccctga
    13441 tcgagcacgt caacgcgctc atccatctgt tgcaggcccc gctctctctg gcgaacgccc
    13501 tcgacgccgc gggcgcggaa gctctcggca gttgcgggag catcctcgac gagcgggcac
    13561 acgtgtgacc agtcggggag gcggagggac ccggacctcc gcctcccggc tgtcctcctg
    13621 tcggacggag gaatcctcag gggcgcggcc ttggatacgg cgacgccccg tcacaattca
    13681 gttgtcaatg aacccgtagc gttgagggac ggctcctcag tgctacggag ccattcctga
    13741 acgcttcgct gccatccctc agtacttcgc cgcgatccct cgacacttcg ccgtcatccc
    13801 tcaaggctga gggatggccc cggagcgttg agggaggact cctcagagtt gagggaacgc
    13861 tcctcactgc ttcgccgccg ttcctcagtg ctccggagcc gcggcgtact actccgccgc
    13921 cgctcctcaa cgttgaggag cggcccctca gtgttccggg atgcctcctc aacacttcgc
    13981 cgtcgttcct cactactttg cggcggtccc tcagtgctcc ggagtcgtcc ctcagcactg
    14041 agggacggct cctcaacact gaggggccgc tccgaagtgt tgaggagccg cggcgaagca
    14101 ttgaggaatg gctccggaat gtccgccgct gctgcctggc tagcagaggc gtggagtaga
    14161 atctacaagg aagacggcgg atgtgtcaat acagtctatc tatgtgacga atatggtagt
    14221 cttggtgaga cggcagggca accggggtga ggtgacgcct gtttgtcggc tctttgacaa
    14281 ttgacgttca ctgttcgacc gcggcgctag ggacggggcg ccgcgtcgga cgctttcttc
    14341 aggaggggac cgtcaaggtc cagaggggga actgaaaagc ctcaatccac ccgcagcacc
    14401 gccaggaagg cctcctgcgg gatgtcgacg cggccgacgc gcttcatccg cttcttgcct
    14461 tccttctgct tcttgatcag cttgcgcttg cgggtgatgt cgccgccgta gcacttggcg
    14521 atgacgttct tcgagatggc gcgcacggtt tcgcgggcga tgacccgcgc gccgatcgcc
    14581 gcctggagca ccacctcgaa gagctggcgg gggatcagct ccttcatctt ctcgaccagc
    14641 accttgccgc gggcgtaggc cttgtcccgg tggacgatca gcgacagggc gtcgaccgga
    14701 tcgccgttga ccaggatgtc cagcttcacc agctcgctgc gccggtagcc gctcatcgag
    14761 tagtcgaagg aggcgtagcc gcgcgagacc gacttcaagc ggtcgtagaa gtcgtggatc
    14821 acctcggcga gcgggaagtc gtactcgatc agcacccggt ccgtggtcat gaagttgagg
    14881 ctccgctgct cgccgcggcg ttcctcggcc agggccagga tgccgccgac gtattccgaa
    14941 cgggtgatga tcgtcgcctg gatgtacggc tcctccaggt agtcgagccg ccccggcgcc
    15001 ggcagcttcg aggggctgtc cacctcgacg cagtcgccgt ccgtggtgtg cgcccggtag
    15061 cgcacccccg gcgcggtggt gatcaggtcg aggttgaact cccgctcgag acgcgcctgg
    15121 atgatctcca tgtggaggag gcccaggaag ccgcagcgga agccgaagcc cagggcgccc
    15181 gagctctccg gctcgaagga gaaggaggcg tcgttcaggc gcagcttctc gaccgcgtcg
    15241 cgcaggtcct cgtagtcgtc cgccgacacc gggtagaggc cggcgaagac catcggcttg
    15301 acctcctgga agcccgggta ggcggcggcc gccggacgcg ccgcgtgcgt cacggtgtcg
    15361 ccgatctggg ccaggagcag gtccttgatg ccggcataga agaagccggc ctcgcctacc
    15421 gagagcgact cgaccgtccc cggcttggga ttgaagatcc ccacctcgtc cacctcatag
    15481 gcccggcgcg acgacatgaa ctggatcttc tcccccttac gcagcgtgcc gtcgacgatc
    15541 cgcaccatgc aggcgacccc ccggtacggg tcgtaccagg agtcgaagag cagcgccttg
    15601 agcggcgcct cggggtcccc ctgcggcgcc gggatgtcgg tcaccacccg ctcgagcacg
    15661 tcgtcgatcc ccagccccgc cttggccgag gccaggacgg cgtcgtgggc atcgatgccg
    15721 atgacgttct cgatctgctc cttgacccgg tcggggtcgg ccgccggcag gtcgatcttg
    15781 ttgatcaccg gcacgatggc gaggttggcg ttgaccgcaa ggtaggcgtt ggccagggtc
    15841 tgcgcctcga ccccctgcgc cgcgtcgacc accaggaccg ccccctcgca ggcggcgagg
    15901 ctgcgcgaga cctcgtaggc gaagtccacg tggccgggcg tgtcgatgag gttgagctga
    15961 taggtctcgc cgtcccgcgc cttgtagaag agacggacgg cgcgcgcctt gatggtgatc
    16021 ccgcgctccc gctccaggtc catgtcgtcg aggagctggt ccatcatgtc ccggtcggcc
    16081 accgccccgg tccgctgcag gatgcggtcc gccagggtcg acttgccgtg gtcgatatgg
    16141 gcgatgatgg agaagttgcg gatgcgctcg tgcggtgcca tgcgtctctc tctgttcggg
    16201 gatttcggca aggctgggcg gccgggccgg ttgccgcgcg tactctaaca agacggagcg
    16261 gagggggaag tatattcccc tccgccccga atccggacgg tcaggcggcc gccctctcct
    16321 cttccaggtc ctccgccccg ggctcggggg acgagccctg ggcggtggag atgagggcga
    16381 cgccggtgac gatcaccgcc gcggcgccca gcatgccggc ggtcagcggc tcgcccgcga
    16441 aggcccagcc gaggaagacg gccaccaccg ggttgacgta cgcgtaggtc gaggcgagca
    16501 ccggccgggc gttgcgcatc agccaggagt agcaggtgaa ggcgatgagg cccccgatca
    16561 ccagatagcc cagagcgagg agcgagcgga gggagatctg gccgacatcg atccgccccg
    16621 gctccccggt gatcgcgctc acggccagca gcaccacccc gcccatcagc atctgcatgc
    16681 ccgaggtgag gatcggggat tcgggcagcg ccgcccgccg ggcatagagc gagccccagg
    16741 cccaggagaa cgaggcggcc gccaggacca ccgcgcccac cggatcgatg ccgccgccgc
    16801 cgggccggat caggaagacc aggccggcga ggcccaggcc caggccggcc aggacccgcg
    16861 ggccgggctt ggcgccgccc ggtgccagcc agaggagcag cacgatccac agcggctcgg
    16921 tggcgaccat cagagccgcc agcccggagt ccacccggtg ctcggcccag accacgccgc
    16981 cgttgccgac ggcgaggagc aggacgccga ccaccgtcgc cggccaccac tgcgccggct
    17041 tgggacgggg gacgccgccc gcgcgggcca tgccgtagag gatcaggccc gagaggacga
    17101 agcgcacg
//
LOCUS       DASPJI010000519         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_665457, whole genome shotgun sequence.
ACCESSION   DASPJI010000519 DASPJI010000000
VERSION     DASPJI010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665457"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..>2348
                     /locus_tag="VEL74_14090"
     CDS             186..>2348
                     /locus_tag="VEL74_14090"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYG63707.1"
                     /translation="MTIDPAWEELSRTLRSSAPYKALLQGAQDVVRLPVPAAAWVGEL
                     LARDLKRPLLVIVPREADALAWIEAARLFGASGGNDRVVYFPAPSLTPYQETETSLLV
                     RAQESVALDCVSSGRARTVVATPRALFRRLPPSKAFAASVLTVRPGEDHPQEELVAHL
                     VRHGFRRTDLVYEVGDFAVRGGIVDLFPPGEDTPVRLDLFGDTVESIRWFDPQSQRSE
                     DTLEAVRVLPLTLFPAGPEEARRLADLLAAGTAAGDGDDPGPETAEMLEGLRRHGTFP
                     GWENYLPLLARATAALSEVLPDPLVLAVDPPALDAEAVHHAERLRADFQARLDHGRLA
                     VPPEEMEEPEERVREILAGAEVRLRDLVMAGGIGRDGESAADFGATPTDLFHGQLPRF
                     PQEVATARARGERCLVVVPPAHRRRMEELLEAREVKLGRGGVELVSGDLDRGFRLPAA
                     GVVVYGEQQLLPQAKLQRRAAARTRYGPFLSSLRDLKVGDFVVHVDHGIGQFVALRSV
                     GRDGDGTGGLPPVLREYAAADAVADVEVMEIAYSGGKRLLLPLSRIDQVQKYSGIEGI
                     APRLDQLGGSSWNRTKARVKKSMRDMAGELLKLYAERQLAAAPAMRDDSDWLHQFEAA
                     FAYEESPDQLDAIATIKEDLQRERPMDRLLCGDVGYGKTEVAMRAAFKAVDNGYQVAV
                     LAPTTILADQHLETFRKRFAGFPVNIEMISRFRTAQEVK"
BASE COUNT          312 a          887 c          832 g          317 t
ORIGIN      
        1 aatcatagag ggcgtcgaca tcgttctcgg tgaatggacg gatgaggaga cgggaaccgt
       61 cccggagaac cgcttcctgt gggtacttga tgggcatctg atccctccgg tgaagtttcg
      121 gacggccgga gcctaacaca agccgaaacg ctctctccgg ggcccgcagg cgatagactg
      181 ttccgatgac gatcgacccg gcgtgggagg agctctcccg gacgctgcgc agcagtgcgc
      241 cctacaaggc cctcctccag ggggcccagg acgtggtgcg cctgcccgtg cccgcggcgg
      301 cctgggtcgg cgagctgctc gcccgcgacc tgaagcgccc cctgctcgtc atcgtccccc
      361 gcgaggccga cgccctggcc tggatcgagg cggcgcgcct gttcggcgcc tcggggggca
      421 acgaccgggt ggtctacttc cccgccccct cgctcacccc ctaccaggag accgagacgt
      481 cgctgctggt gcgcgcccag gagtcggtcg ccctcgactg cgtctcctcc ggccgggcgc
      541 ggaccgtggt cgccaccccg cgcgccctgt tccggcggct gcccccgtcc aaggcgttcg
      601 ccgcgtcggt gctgaccgtc cgcccgggcg aggaccaccc gcaggaggag ctggtggccc
      661 atctcgtccg ccacggcttc cggcgcaccg acctggtcta cgaggtgggg gacttcgccg
      721 tccgcggcgg catcgtcgac ctcttccccc cgggggagga cacgccggtg cgcctcgacc
      781 tcttcggcga cacggtggag tcgatccgct ggttcgatcc gcagtcgcag cgctccgagg
      841 acaccctgga ggcggtccgc gtcctgccgc tcaccctctt cccggccggc ccggaggagg
      901 cgcggcggct ggcggacctg ctcgccgccg ggacggccgc cggcgacggc gacgatccgg
      961 ggcccgagac cgccgagatg ctcgaggggc tgcgccggca cggcaccttc cccggctggg
     1021 agaactacct gccgctcctc gcccgcgcga ccgcggcgct ctccgaggtg ctgcccgacc
     1081 cgctggtcct cgccgtcgac ccgccggcgc tcgacgccga ggcggtccac cacgccgagc
     1141 gcctgcgcgc cgacttccag gcccgcctcg accacgggcg cctcgccgtg ccccccgagg
     1201 agatggagga gccggaggag cgggtgcgcg agatcctcgc cggcgccgag gtccgcctgc
     1261 gcgacctggt gatggcgggg ggaatcggca gggacggtga gagtgccgcc gacttcggcg
     1321 ccaccccgac cgacctcttc catggccagc tgccgcgctt cccgcaggag gtggcgaccg
     1381 cccgcgcccg cggcgagcgc tgcctggtgg tcgtcccccc cgcccaccgg cggcggatgg
     1441 aggagctgct cgaggcgcgc gaggtcaagc tcggccgcgg cggcgtcgag ctggtctcgg
     1501 gagacctgga ccgcggcttc cggctgcccg ccgccggcgt ggtggtctac ggcgagcagc
     1561 agctcctccc ccaggccaag ctgcagcgcc gcgccgccgc ccgcacccgc tatggcccgt
     1621 tcctctccag cctgcgcgac ctcaaggtgg gggacttcgt ggtccacgtc gaccacggca
     1681 tcggccagtt cgtggcgctg cgcagcgtcg gccgcgacgg cgacggcacg ggcggcctgc
     1741 cgccggtgct gcgcgagtac gcggcggcgg acgcggtggc cgacgtcgag gtgatggaga
     1801 tcgcctactc cggcggcaag cggctgctcc tgccgctgtc gcgcatcgac caggtccaga
     1861 aatacagtgg catcgagggg atcgcgccgc gcctcgacca gctcggcggc tcgtcgtgga
     1921 accgcaccaa ggcgcgggtc aagaagagca tgcgcgacat ggcgggggag ctgctcaagc
     1981 tctacgccga gcgccagctc gccgccgcgc cggcgatgcg cgacgactcc gactggctgc
     2041 accagttcga ggccgccttc gcctacgagg agagcccgga ccagctcgac gccatcgcca
     2101 ccatcaagga ggacctccag cgcgagcggc cgatggaccg gctgctgtgc ggcgacgtcg
     2161 gctacggcaa gaccgaggtg gcgatgcgcg ccgccttcaa ggcggtggac aatggctacc
     2221 aggtggcggt cctcgccccc accaccatcc tcgccgacca gcacctggag accttccgga
     2281 agcgcttcgc cggcttcccg gtcaacatcg agatgatctc gcgcttccgc accgcgcagg
     2341 aggtgaag
//
LOCUS       DASPJI010000520         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_32464, whole genome shotgun sequence.
ACCESSION   DASPJI010000520 DASPJI010000000
VERSION     DASPJI010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32464"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..502)
                     /gene="rimI"
                     /locus_tag="VEL74_14095"
     CDS             complement(50..502)
                     /gene="rimI"
                     /locus_tag="VEL74_14095"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HYG63708.1"
                     /translation="MSAESLLIIRPARLDDLDRIAALEQSGFPDPWPADLLAYELRHP
                     GAILLVAAWGEGPAVGYAAFRHGGGEAELLRLSVDPAERRRGLGQALVNRGLERLREI
                     GVESCFLEVRLDNEGAIACYRSLGFTLTGMRRAYYRDGSDALVYSRRI"
     gene            complement(499..1224)
                     /gene="tsaB"
                     /locus_tag="VEL74_14100"
     CDS             complement(499..1224)
                     /gene="tsaB"
                     /locus_tag="VEL74_14100"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HYG63709.1"
                     /translation="MPAVPTAAAPLVLVLDSGAPLVSVTVGRPSKEGGGEVLALRSME
                     IARSSRQLLEMVGEVLAEVGARPRDLGGVAALRGPGSFTGLRIGLSTVLGLHQALGLP
                     ATAVSTLAALAASLPSEEGGLVVAAVDALRGEWSAQAFAVGAGGSAPRPLTGIELVPG
                     SELPGLVGGTAVVTGFGVGRLAELPGWPAGVRLHEAGPLAPAGLRLAGPPGPAWDPAL
                     LTSPFYSRPPAVTVPRERVPATP"
     gene            1310..>2812
                     /locus_tag="VEL74_14105"
     CDS             1310..>2812
                     /locus_tag="VEL74_14105"
                     /inference="COORDINATES: protein motif:HMM:NF017043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HYG63710.1"
                     /translation="MSSLPQTSLTPMLRQYLEIKAQYPDTILMYRMGDFYELFFEDAV
                     RAAPLLEVQLTARHKGTESETPMCGVPHHALESYLAKLIGAGLRVAICDQVEDPAQAK
                     GLVKREVTRVVTPGTLSDPALLEGKEENLLAGLAWDRQEGAGAFLDVSTGSFFVRRWR
                     GPEEAVDDLALMRPREVLLHGPGGAAGFPAPLLEWAERECPCRTPLDGDRLFDPERAG
                     ELLLRQFGAGTLRGFGLEEREPAVRAAAAALAYARETQKSDLTHIRGLSVREARDRMI
                     LDASTLSNLEVFQSQRSGMAGPGTGAAAGRRKGSLLAVLDRTVTAPGGRALREWLRRP
                     LVDPDEIAARHRAVGELVADTPRRERLRERLARVGDPERLLTRAVLGTFTPREAAALR
                     DGLAEVPGILAELATVPMEASPLLFELAGTDPLAGLHAELARVLEEAPAPSLQDGGVI
                     AAGVDEDLDRYRSLARDSKRHILALESRERQRTGIGSLKIRYNKVFGYYLE"
BASE COUNT          389 a         1097 c         1002 g          324 t
ORIGIN      
        1 gccacgctgc cagtcgcctg cgccgggcga agccggggcc gtacgggcct cagatccgcc
       61 gggagtacac cagcgcgtcc gagccgtcac ggtaataagc acggcgcatg ccggtcagcg
      121 tgaagccgag gctgcggtag caggcgatgg ccccctcgtt gtccagccgc acctccagga
      181 agcagctctc gaccccgatc tcccgcagcc gctcgagccc ccggttgacc agcgcctggc
      241 cgaggccccg gcggcgctcg gccgggtcca cggacaggcg gagcagctcc gcctcgcccc
      301 cgccgtgccg gaacgccgcg tacccgaccg ccggcccctc cccccaggcc gccaccagca
      361 ggatcgcccc ggggtgacgc agctcgtagg ccaggaggtc ggcgggccag ggatcgggga
      421 agcccgactg ctcgagcgcg gcgatccggt ccaggtcgtc gaggcgggcc ggccggatga
      481 tcagcaggga ctccgcgctc atggcgtcgc cggcaccctc tcccgcggga cggtgaccgc
      541 cggcggacgg gagtagaagg gcgaggtgag gagcgccggg tcccacgcgg gtcccggagg
      601 gcccgccagg cgcagccccg ccggcgccag cggtcccgcc tcgtggagac ggacgcccgc
      661 cggccagccg ggcagctccg ccagcctgcc gacgccgaag ccggtcacca cggcggtccc
      721 ccccacgagg cccggcagct cgcttccggg gaccagctcg atcccggtca gcggacgggg
      781 ggccgagccg cccgctccca cagcgaaggc ctgggcgctc cactcccccc gcaaggcgtc
      841 caccgccgcc accaccaggc ctccttcttc agaaggaagg gacgccgcga gcgccgcgag
      901 cgtcgagacg gcggtggccg gcaaacccag ggcctggtgc agccccagga ccgtcgacag
      961 gccgatgcgc aggccggtga agctgcccgg tccgcgcagc gccgccaccc cacccagatc
     1021 gcgaggccgc gcccccacct cggcgagcac ctcgcccacc atctccagga gctgccggga
     1081 cgagcgcgcg atctccatcg agcggagcgc cagcacctcg ccgccccctt ccttggaagg
     1141 acggcccacg gtcacgctca ccaacggcgc gccgctgtcg aggacgagga cgaggggggc
     1201 ggcggcggtg gggacggcgg gcatggaggc gaaaatataa gaaagccggc gagcgcgcac
     1261 gtggtccttg ggacgagtcc gggctcatgg gcggatatac tcccttccca tgtcctcgct
     1321 cccccagacg tctctcacgc cgatgctgcg ccagtacctc gagatcaagg cgcagtaccc
     1381 cgacaccatc ctcatgtacc ggatgggtga cttctacgag ctcttcttcg aggacgccgt
     1441 gcgcgccgcg ccgctgctcg aagtccagct caccgcccgc cacaagggga ccgagagcga
     1501 gacccccatg tgcggcgttc cccatcacgc tctggagtcc tacctggcca agctcatcgg
     1561 cgccggactg cgggtcgcca tctgcgacca ggtggaggac cccgcgcagg ccaaggggct
     1621 ggtgaagcgg gaggtgacgc gggtggtcac gccggggacg ctctccgacc cggcgctcct
     1681 cgagggcaag gaggagaacc tcctggccgg gctcgcctgg gaccgccagg agggggcggg
     1741 ggccttcctg gacgtctcga ccggcagctt cttcgtccgc cgctggcgcg gccccgagga
     1801 ggcggtcgac gacctggcgc tgatgcgccc ccgcgaggtg ttgctgcacg gccccggcgg
     1861 tgccgccggc tttcccgctc ccctcctgga gtgggccgag cgggagtgcc cctgccgcac
     1921 cccgctcgac ggcgaccgcc tgttcgaccc cgagcgggcc ggcgagcttc tgctgcgcca
     1981 gttcggcgcc ggcaccctgc gcggcttcgg cctggaggag cgggagccgg ccgtgcgcgc
     2041 cgccgccgcc gccctcgcct acgcccggga gacccagaag agcgacctga cccacatccg
     2101 cggcctctcc gtgcgcgagg cccgcgaccg gatgatcctc gacgcctcga cgctgtcgaa
     2161 cctggaggtc ttccagagcc agaggtccgg gatggcgggg ccgggcaccg gagcggcggc
     2221 gggccgccgc aagggctccc tgctcgccgt cctcgaccgc acggtcacgg cgccgggcgg
     2281 ccgggccctg cgggagtggc tgcgccggcc gctggtggac ccggacgaga tcgccgcccg
     2341 ccatcgcgcc gtgggcgagc tggtagccga caccccccgg cgggagcggc tgcgcgagcg
     2401 cctggcccgg gtgggcgatc ccgagcgcct gctgacccgg gcggtcctcg gcaccttcac
     2461 ccctcgcgag gcggcggcgc tgcgcgacgg cctcgccgag gtccccggca tcctcgccga
     2521 gctggcgacg gtccccatgg aggcctcccc ccttctcttc gagctcgccg gtaccgaccc
     2581 gctggccggc ctgcacgccg agctggcccg ggtgctggag gaggcgccgg cgcccagcct
     2641 gcaggacggc ggggtcatcg ccgccggcgt cgacgaggat ctggaccgct accggtccct
     2701 cgcccgcgac agcaagcggc acatcctggc cctggaaagc cgcgagcgcc agcgcaccgg
     2761 catcggctcg ctcaagatcc gctacaacaa ggtcttcggc tactacctcg ag
//
LOCUS       DASPJI010000521        11869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_202885, whole genome shotgun sequence.
ACCESSION   DASPJI010000521 DASPJI010000000
VERSION     DASPJI010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11869)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11869
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202885"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VEL74_14110"
     CDS             complement(<1..863)
                     /locus_tag="VEL74_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYG63711.1"
                     /translation="MSLVLDRIPQNLLAVTPPSLASVLTEVAETAAETLELQEVFDRV
                     ATAIHRLIPFDNMGVVRILEGQWAVLHATTVPCPETDAKCTEPEPLTDWSPRFRPRPG
                     PIPRLDDAQLELDPTFPLDANLLTWGVRSALWEPFRMAEGFSGGVWLCAYTPYAFTEE
                     HQELLRPIAAILGSAVEHWRIWDSERRRRERLDRLETLVGALAETLDIRDVFTQLSAA
                     VQPVLPHHLLVLTELDLRARSFRITAYAGEADVEVPTGALVLTEEETRQRSQDFELVC
                     DIQTEMSAKTER"
     gene            1057..2568
                     /locus_tag="VEL74_14115"
     CDS             1057..2568
                     /locus_tag="VEL74_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HYG63712.1"
                     /translation="MDDRLDEIGSSLDSTGIAGLDDVLAGGFPPHQLYLVEGRPGTGK
                     TTLALQFLLAGRARGETVLYVTLSETRRELLAVGRSHGWSLDGIHIHEMEPLEESLAP
                     EQQLTIFHPSELELSETTSKVLKTVEEIRPSRVVFDSLSEMRLLAQSSLRYRRQILGL
                     KQFFAGRQSTVLLLDDLTGPGDDQQLQSIAHGVLHLEQLATEYGAERRRLRVLKMRAT
                     AFRGGLHDFVIRRGGLDVFPRLVAAEHHTDFEDREASSGIAGLDRLLGGGLPFGSSTL
                     LLGPAGTGKSSICLSFCVAAAARGERSSLFLFDETVGMIKARARKLNMPLEPHIEAGL
                     ITMRQIEPAELSPGEFAYSIRQAVEGTDGTGSPAKLVVLDSLNGYLNSMSEEKQLTSQ
                     LHELFKYTSHQGVLMLVTLTQAGMGGTATRTPVDSTYLADNVVIFRNFEAFGQLRRAI
                     AVTKKRSGEHELTLRELRMSSQGISISEPLNEFQGVLTGVPTYHGPGDPRNQR"
     gene            2565..4127
                     /locus_tag="VEL74_14120"
     CDS             2565..4127
                     /locus_tag="VEL74_14120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG63713.1"
                     /translation="MTQERSPAELRVLLLPLTARDGQASHQILASGGIHCEVFKELED
                     LRTELAVGAAVLVLPEEVVLADTAATLQQSLRLQPAWSDLPVIVLARAGRQSPEVERA
                     LSTLGNVSLVERPLRVSTLVSQVRSALRAREHQYQVRDLLTDERAARIEADVANRAKD
                     RFLAVLSHELRTPLSPVVMGLAAMEADARLPPALLSDVSMMRRNIDLEVKLIDDLLDM
                     SRVATGKLRLQREPVGVHAVLRHVFEICADDLGSKHLVLAVDLGAVNDRVAADAARLQ
                     QVFWNLLKNAVKFTPVDGTISVRTLTPGAGRLGIEIRDSGVGISADVLPKVFDAFEQG
                     NLRASGQSGLGLGLAICKALVELHGGTIHAASAGDGQGACFTVELATARPEEAAEKAS
                     GAVTATPGGVRPRLLIVEDHEDTAMILARLLQSSGYEVTEAHTVAEALAATAAAPFDL
                     LISDIGLPDASGLDLMRQIRARFGIPGIALSGYGMEEDIRHSRDAGFLDHIVKPINLD
                     HLKAAIQRVIAR"
     gene            4317..4841
                     /locus_tag="VEL74_14125"
     CDS             4317..4841
                     /locus_tag="VEL74_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63714.1"
                     /translation="MRSSVDGSSVSNPPSLPNAFTAAFLRRLLERDESPTGAEADVAG
                     PWRIEEVPGRGFGLFRVGESAARGFEPVALYPTCWLALVTAAVLPGTGRDPLLRLRFD
                     AAEDGRYEITLEDGEVVGSFQSFDEKLLDAVNTGIALVRAPLSLADLLEAAGPVGAER
                     CGTILEERISQIPE"
     gene            5009..5458
                     /locus_tag="VEL74_14130"
     CDS             5009..5458
                     /locus_tag="VEL74_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63715.1"
                     /translation="MLRNAVSILALLLALVLLLLVVPPSSSFQLKEVMTAAEFERCGL
                     QKLTRSELATLEGWISRRAGQSPEETVRPKPVDPVEVPLGLPNGTDAGATKKSDLVMF
                     NQSSKKYHCPSCRWAKECTRNCVEITRAEALRLGGVACKTCKGRCAE"
     gene            complement(5776..6651)
                     /locus_tag="VEL74_14135"
     CDS             complement(5776..6651)
                     /locus_tag="VEL74_14135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG63716.1"
                     /translation="MAWDVRGKRVLITGATSGIGLEASVELARRGARVVMVGRDPGKT
                     ERAVAEVKGRSGSGEVSFLLCDFSSQAEIRRLAQEVLASHERLDVLVNNAGAVNKERR
                     VTGDGIEMTFAVNHLGYFLLTHLLTDLLARSAPARVVSVASEAHRDGTLDFEDLGFER
                     GYTIMRAYSRSKLANVLFSNELARRLAGTGVTSNCLHPGVVATNIWSGAPGWIRPVIQ
                     LVALAFFISPEKGATPIVHLVAGPDLERVTGQYFVRHKPALPALIARDETVARRLWRV
                     SGEMVGLPEVQSRVP"
     gene            complement(6739..7023)
                     /locus_tag="VEL74_14140"
     CDS             complement(6739..7023)
                     /locus_tag="VEL74_14140"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYG63717.1"
                     /translation="MLLIGTDSGVPLNFHSDSTWREIDAWVNTFGVPAMDAIRAATFW
                     PAVAMKVDKEVGTVSAGKYADIIAVRGDVLRHVDLLQDVDLVIERGVRYR"
     gene            complement(7146..7613)
                     /locus_tag="VEL74_14145"
     CDS             complement(7146..7613)
                     /locus_tag="VEL74_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63718.1"
                     /translation="MTYPHAEVIEFVTKHFAAAKFNVKEPVPDFKEAVGRGKVIWAPL
                     FIFLDYRGAELRRYLGYLPHDHFLAELRLVLGLAAMTHMKYQEALEWFLSAVEKSPDS
                     SAAPEALFWAGAADYRIGGAQPMIARWDDLIARYPGSTWATRADIIPPELRER"
     gene            complement(7759..9135)
                     /locus_tag="VEL74_14150"
     CDS             complement(7759..9135)
                     /locus_tag="VEL74_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63719.1"
                     /translation="MITITAQDLRGAVILSAVFLSLIVFAELWRRFGNPDAEWTRKLV
                     HLGGGITCLLFPFRVRSPWVVLAMALPLTVLFVAGSKLGFLKSLHGVKRRSRGAEYYP
                     LAVFLTFVLTQGRPWLYLSAVLVLAVADAFAALIGSQYGRVRYEVEDETKSLEGSLVF
                     LVLAFLAIHLPTLLLTDLPRPVCVLAALLVAALVTGFEAISLEGADNLFIPLAVVLIL
                     AKITSKPLGEVIFQNLSLLAICLLVALVVWRSHSLNVGGAIAFILFAYGAWSLGNWRW
                     ALPALVGYGLYVLLWYRFAPPGHASGLKVRILVRAVLPAFAVLAAANGLGRGPLFYGP
                     YLAAIAAALAFSLDTGVFRLERRQGMSWIAGALGLGALAALATALVPWLLQRREVPLQ
                     LLLVVGVTMAVSLFNAALELRDPDLPRSRDWTAWRFLLSFAAAGAVLGLQALGTAPLW
                     EVGRGLVR"
     gene            complement(9132..10250)
                     /locus_tag="VEL74_14155"
     CDS             complement(9132..10250)
                     /locus_tag="VEL74_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013556322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HYG63720.1"
                     /translation="MADRIEKRARFDLIRYANVWEDAQVLCAALRPAPGQRILSIASA
                     GDNSFSLLSHGAHVLAVDLSPAQLNLVELKKTAVRHLDYETALTFLGVRPSASRLAVY
                     GHLAKHLSDQAREFWGDRPEAIEGGVIHHGKFESYFHLFRRRVLPLVHGRRTVERLLE
                     EKDEAARRKFYGRVWDTWRWKLLFRVFFSRFTMGRLGRDPEFFRYVEGSVAERILGRT
                     RYALTVLPPHANPYVDYILTGSFTHTLPHWLRPEVFPKVRENLDRLTLFEGSVEPVAE
                     AEWLARGEGFDGFNLSDIFEYMDPATSAQVYGRLLATARPGARFAYWNMLAPRRLAPS
                     FPGRVRPLDDEAGQLFARDLAFFYSAFVIDEVVDEVTG"
     gene            complement(10269..11120)
                     /locus_tag="VEL74_14160"
     CDS             complement(10269..11120)
                     /locus_tag="VEL74_14160"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HYG63721.1"
                     /translation="MSHDLEMEDDLTTPLKAAVDRWDDLGWPRPKVAMVTGSGLGIDM
                     EDRIHGPVSLEYFLPFGLHPVEGHAHELDVLLPRPDLPVLYQRGRLHSYQGYSAHDTV
                     FPVRFAALLGAKVLILTNATGGLQPGQKPGDLVLIRDHINLTGLNPLRGQLPSAWGPR
                     FPDMSAAYDLELRALTLAAAAELGITLTEGIYAGVAGPSYETPAEVRMLRQLGGDVTG
                     MSTVLEVIAARHMGMRCLCVSMVSNPAAGMSEAAVDHADVLAAAQAAAIDLRRLLRAV
                     LESPDLG"
     gene            11233..>11869
                     /locus_tag="VEL74_14165"
     CDS             11233..>11869
                     /locus_tag="VEL74_14165"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HYG63722.1"
                     /translation="MSELDEQIQNRREKRQRLAEAGVQVYPHRFEWDLEPAGIHARYG
                     DKSAEELDQAALRLRVPGRVRSIREHGKTAFVDLHDGKEKLQLFIRKDRLSETGRLVL
                     ENLDLGDLVGAAGTLMRTRMGELSLSPDDLTLLAKAVRPMPEKWHGLADIEVRYRQRY
                     LDLATNPESRQVFETRAAIVRGIREFLDRHGFLEVETPMMQILPGGATARPF"
BASE COUNT         1980 a         3980 c         4144 g         1765 t
ORIGIN      
        1 cgctcggtct tggcgctcat ctcggtctgg atgtcgcaca cgagctcgaa gtcctgggac
       61 cgttggcgcg tctcctcctc ggtgagcacc agcgccccgg tggggacctc gacgtcggcc
      121 tcgccggcgt aggctgtgat ccggaaggaa cgggcgcgga ggtccagctc ggtgagcacc
      181 agcaggtgat gcggcaggac aggctgcacg gccgccgaga gctgcgtgaa cacgtcgcgg
      241 atgtcgagcg tctcggcgag cgcaccgacc agcgtctcca ggcggtcgag gcgctcccgg
      301 cggcgacgct cggaatccca gatgcgccag tgctcgaccg ccgagccgag gatcgcggcg
      361 atcggccgca ggagctcctg gtgctcctcg gtgaatgcgt agggggtgta ggcgcacagc
      421 cagacgccgc cgctgaagcc ctccgccatg cggaacggct cccagagagc cgaccggacc
      481 ccccacgtaa ggaggttggc gtcgagcggg aaggtggggt ccaactcgag ctgggcgtca
      541 tcgaggcggg ggatcggccc cggccgaggg cgaaatctgg gcgaccagtc ggtcaacggc
      601 tcgggctccg tgcacttggc gtcggtttcc gggcacggca cggtggtcgc gtgcagaacc
      661 gcccactgtc cttcgaggat ccgcaccacc cccatgttgt cgaaggggat gagccggtgg
      721 atcgcggtcg ccacccggtc gaacacctcc tgcagctcca gcgtctcggc ggcggtctcg
      781 gcgacctccg tcagtacgct ggccagcgag ggcggggtga ccgccagcag gttctgcggg
      841 attcgatcca ggaccaggct catgcccctt accttagcac cgccgcgagg aggtcgccaa
      901 cggtaaacga gatggcggag cggaccgaat gggtacctgc ggaatcgtct ggcatgaaag
      961 tggcagagtg ccatccggac atgtcgagcc gatgcgattc agataggatt ctgcacagca
     1021 tctgtgtagc ttggcggaca tggacggaga ggcacagtgg acgatcggct cgacgagatc
     1081 ggtagctctc tagactctac cggcattgcc ggcttggacg acgtcctcgc cggcggattt
     1141 cctccccatc agctctatct ggtcgaaggc aggcccggca ccggcaagac caccctggcc
     1201 ttgcagtttc tcctggcggg ccgggcgcgc ggtgagaccg tgctctacgt caccttgtcg
     1261 gagacgcggc gcgagttgct agcggtgggc cgcagccacg gctggagcct cgacggcatc
     1321 cacatccacg agatggagcc gctcgaagag agcctggccc cggaacagca gctcaccata
     1381 ttccacccgt ccgagctgga gttgagcgag accaccagca aggtgctgaa gacggtggag
     1441 gagatccggc cgtcgcgggt ggtcttcgat tccctgtcgg agatgcgctt gctcgcgcaa
     1501 agctcgttgc gctaccgccg gcagatcctg ggcctcaagc agttcttcgc cggccggcaa
     1561 tccacggtgc tgctcctcga cgacctcacc gggccggggg atgaccagca gctccagagc
     1621 atcgcccacg gcgtcctcca cctggaacag ctcgccaccg agtacggagc ggagcggcgc
     1681 cggctgcggg tgctgaagat gcgggccact gcgttccggg gagggctgca cgatttcgtc
     1741 atccgccggg gcggcctcga tgtctttccg cggctggtcg ccgccgagca ccacaccgac
     1801 ttcgaggacc gggaggcgtc gagcggcatc gccgggctcg accggctgct cggcggcggg
     1861 ctccccttcg gcagcagcac gctcctgctg ggtcccgcgg gcaccggcaa gtcgagcatc
     1921 tgcctgagct tctgtgtcgc cgcggcggcg cggggggaac gttcgtccct gttcctcttc
     1981 gacgagaccg tgggcatgat caaggcccgg gcgcgcaagc tcaacatgcc cctggagccc
     2041 catatcgagg ccggcctcat cactatgcgg cagatcgagc cggccgagct gtcgccggga
     2101 gagttcgcct actccatccg gcaggcggtc gaagggacgg atgggacggg ctcgccggcc
     2161 aagctggtcg tgctcgatag cctgaacggg tacctgaact cgatgtcgga agagaagcag
     2221 ctcacgtcgc agctccacga gctgttcaag tacaccagcc accagggcgt cctgatgctc
     2281 gtgaccttga cccaggcggg catgggagga acggcgacgc gcaccccggt cgactcgacc
     2341 tacctggccg acaacgtcgt gatattcagg aacttcgagg ccttcggcca gctccggcgc
     2401 gccatcgcgg tgaccaagaa gcggagtgga gagcacgagc tgaccctgcg cgagctccgc
     2461 atgagctccc agggcatctc catcagcgag ccgctgaacg agttccaggg agtgctgacg
     2521 ggggtgccga cctatcacgg gccaggggat ccaaggaacc agcgatgacc caggagcgca
     2581 gccccgcgga gctgcgggtg ctcctgctcc ctctgactgc gcgggacggg caagccagcc
     2641 accagatcct ggcgtccggg ggcatccatt gcgaggtctt caaggagctg gaggatctgc
     2701 ggaccgagct ggcggtcggc gccgcggtcc tcgtcctgcc ggaagaggtg gtcctcgccg
     2761 acaccgccgc gacgctccag cagtccctcc gcctacagcc ggcctggtcg gacctgcccg
     2821 tcatcgtgct ggcgcgcgcc gggaggcaat cgcccgaggt ggagcgcgcg ctttccaccc
     2881 tgggcaacgt cagcctcgtc gagcggccct tgcgggtctc gaccctggtg agccaggtca
     2941 ggtctgcgtt acgggcccgc gagcaccagt accaggtccg tgacctgctc acggacgagc
     3001 gggcggcgag gatcgaagcc gacgtcgcga accgggccaa ggatcgcttc ctcgccgtcc
     3061 tcagccacga gttgcggacg ccgctgtcgc cggtcgtgat gggcctcgcc gcgatggagg
     3121 cggacgcgcg gctgccgccg gcactgctga gcgacgtatc gatgatgcgc cggaacatcg
     3181 atctggaggt caagctgatc gatgacctgc tcgacatgag ccgggttgcc accggcaagc
     3241 tgcggctgca gcgggaaccg gtcggcgttc atgccgtcct ccggcatgtc ttcgaaatct
     3301 gcgcggacga cctgggatcg aagcacctgg tcctggccgt ggacctgggc gccgtgaacg
     3361 accgtgtggc cgcggatgcg gcccggttgc agcaggtgtt ctggaacctc ctgaaaaatg
     3421 ccgtgaagtt cactccggtg gacgggacga tctccgtgcg caccctgacg ccgggcgcag
     3481 ggcggctcgg gatcgagatc cgggacagcg gtgtcggcat ctcggccgac gtgttaccga
     3541 aggttttcga cgccttcgag caggggaatc tcagagccag cggtcagtcc ggtctgggcc
     3601 tgggccttgc catctgcaaa gcgctggtgg agctccacgg cggcacgatc cacgcggcga
     3661 gcgccggcga cggccagggc gcctgcttca cggtcgaact ggcgaccgca cgaccggagg
     3721 aggcggcgga gaaggcgagc ggggcggtga cggcgacgcc cgggggcgtg cggccccgcc
     3781 ttctgatcgt cgaggaccac gaagacaccg ccatgatcct ggcgcggctg ctccagtcct
     3841 ccggttacga ggtgacggag gcccacacgg tcgcggaggc gctggcggcg accgctgccg
     3901 cgccgttcga cctcctgatc agcgacatcg gcctgccgga cgcgagcggc ctggacctca
     3961 tgcggcagat ccgcgcccgc ttcgggattc ccggcatcgc cctctccggc tatgggatgg
     4021 aagaggacat ccggcacagc cgtgacgcgg ggttcctgga tcacatcgtg aagccgatca
     4081 acctcgatca tctcaaggcc gccattcaac gcgtgatagc ccgttgactt ccgtcccgtt
     4141 ctgcttctac aatcccatca tcaggcagta cccgcaggac acgggggtcg cgctcgctgg
     4201 gaagatgagc ccgccctccg gagaagacgc tgtttcacaa caacttgcgg agacggcccg
     4261 gggggtgagt tggaggctca actcccggac ctgaccgcgc aaaaggagag aacccaatgc
     4321 gcagctcagt agatggtagc agtgtgtcca atcccccttc cctccccaac gccttcaccg
     4381 cggccttcct gcggcgtctc ctggagcggg acgagtcgcc cacgggggcg gaggcggacg
     4441 tggcgggtcc ctggcggatc gaggaggtcc cgggccgtgg cttcggcttg ttccgggtgg
     4501 gggagagcgc ggcccgcggg ttcgagccgg tggcgctcta tcccacgtgc tggctggcgc
     4561 tggtcaccgc ggcggtcctc cccggtaccg gccgtgaccc gctgctccgg ctccgcttcg
     4621 acgccgccga ggacgggcgc tacgagatca ccctggagga cggcgaggtc gtgggcagct
     4681 tccagagctt cgacgagaag ctcctggacg ccgtgaacac cggcatcgcc ctggtgagag
     4741 cgccgctctc cctcgcggat ctgctggaag cggccgggcc ggtaggcgcc gagcgctgcg
     4801 gcacgatcct cgaagagcgc atctcacaga tccccgagta gatcccccgc cgccgccggt
     4861 gcgtctcgtg ccggcggcgg tacgtctcat cgacccggcg ggatggctcg gccggcggcc
     4921 ccatgatcta ccccacagag cctgctcaac caagagcgcc gcggtggtgt cttttctggc
     4981 aggcccgtca ttggccgtgg gagatgtcat gctgcggaac gccgtatcca tcctggccct
     5041 gctcctcgcc ctcgtcctcc tgctcctcgt cgtcccgccc tcctcttcct tccagctcaa
     5101 ggaggtcatg accgcggcgg agttcgagcg ctgtggcctt cagaagctca cccgctcgga
     5161 gctggccact cttgaaggct ggatcagccg ccgagccgga cagtcccccg aggagaccgt
     5221 gaggcccaag cccgtcgatc ccgtggaggt tccgctggga ttaccgaacg ggacggatgc
     5281 cggagctacg aagaagtcgg acctcgtgat gttcaaccag agctcgaaga agtaccactg
     5341 tccgtcttgc cgatgggcca aggagtgcac ccgcaactgc gtggagatca cccgcgccga
     5401 ggccctgcgg ctgggcggcg tggcctgcaa gacgtgcaag ggacggtgcg cggagtaggg
     5461 cagccgttgc cccgcgagag aggaccgtcc taccggggag aaggccgccg tacccgttgg
     5521 gatcgtcgtc gtacccctcg aaaaggccgc catacccggg gtagggaggt catccctccg
     5581 gggatcgtcg tcgtacccgc gagaatgtcc ttcacggcct ggggatgagg gccatctctc
     5641 cgggtatgac cgccgtaccc cgggtagggc ctccataccc tgggtagaga ggctatacct
     5701 ccagggatga cggtcacaca cccggggata gtcgtcggaa aacggagaaa actccccttc
     5761 gatccaaggc agcggctacg ggacccgact ttgtacctcg ggcagaccga ccatctcccc
     5821 gctcaccctc cagaggcgtc tcgccaccgt ctcatcccgc gcgatcaagg ctggtagagc
     5881 tggtttgtgc cggacgaagt attggccggt gaccctttcg aggtcgggac cggccaccag
     5941 gtggacgatg ggggtggcgc ccttttcggg gctgatgaag aacgctaggg ctacgagttg
     6001 gatcacgggc cggatccacc ccggcgctcc cgaccagatg ttcgtcgcca cgactccggg
     6061 atgcaggcag ttggaggtga cgcccgtgcc cgcgaggcgc cgcgccagct cgttggaaaa
     6121 gaggacattg gcgagcttcg agcgcgagta ggcgcgcatg atggtgtagc cccgctcgaa
     6181 tccgaggtcc tcgaaatcga gggtcccgtc cctgtgagct tcggaggcga ccgagactac
     6241 ccgggccggg gcgctccgtg cgaggagatc ggtgaggagg tgggtgagca ggaagtagcc
     6301 gagatggttg acggcaaacg tcatctcgat cccgtccccc gtcacccgcc gttccttgtt
     6361 gaccgctccc gcgttgttga ccagaacgtc gagccgctca tggctggcga ggacctcctg
     6421 ggcgagccgg cggatctccg cctgggacga gaagtcacag aggaggaacg acacctcgcc
     6481 cgacccgctc cgccccttca cctccgccac cgccctctcg gtcttcccgg ggtcccgccc
     6541 gaccatcacc acccgcgccc cccgccgggc cagctccacc gaagcctcca gcccgatccc
     6601 gctcgtcgcc cccgtgatca gcacccgctt cccgcgtacg tcccaggcca tgagccctcc
     6661 ctcccacgtg tgagaggacg ctaccggagg gcggggggcg agtcaaacgg gggagaaggc
     6721 ggggacgggg gccgtcccct acctataccg caccccccgc tcgatcacca aatccacatc
     6781 ctgcagcaga tccacatgcc gcaacacatc gccccgcacg gcgatgatgt cggcgtactt
     6841 gccggcggag acggtgccga cctccttgtc cactttcatg gcgacggcgg gccagaaggt
     6901 ggcggcgcgg atggcgtcca tggcggggac tccgaaggtg ttgacccagg cgtcgatctc
     6961 gcgccaggtg gagtcgctat ggaagttcag ggggacgccg ctgtcggtgc cgatgaggag
     7021 gatggcgccg gactggcgga gctgctcgga tcagcggcga aggctggaac gatcagacac
     7081 gtcaggatca gaacacaaag cacgactctt ggcatgaacc cctccggaaa gaaaataaga
     7141 gatcttcacc tctccctcaa ctccggcgga atgatgtccg cccgcgtggc ccaggtgctt
     7201 ccgggatagc gggcgatcag atcatcccag cgcgcgatca tgggctgggc gcctccgatc
     7261 cggtaatctg cggctccggc ccagaagagg gcttccggag cggcactgga atcgggggat
     7321 ttctcgacgg cgctcaggaa ccactccaac gcctcctgat acttcatgtg cgtcatggcg
     7381 gccaagccca ggacgagacg aagctcggca aggaagtggt cgtgtgggag atagccgaga
     7441 tagcggcgca actcggcgcc gcgatagtcg aggaagatga agagcggcgc ccagatgacc
     7501 ttgccgcggc ccaccgcttc cttgaagtcc ggcaccggct ccttgacgtt gaacttggcg
     7561 gcggcgaaat gcttcgtcac gaattcgatc acttcggcgt gcgggtacgt cacggtaccc
     7621 atcttgcggc agccgagtca gccgggggtc cagaaatcga ggagcaccgg cttcctggtg
     7681 cgctccgcct cgtcctttgc cggttggagc cggtcgtgcc attggatctc ggacattgtc
     7741 ttgctctcct attgatcgtt atcgtaccaa cccccgcccc acctcccaaa gcggcgctgt
     7801 tcccagcgcc tgcaagccca ggaccgctcc ggcggcggcg aagctgagga ggaagcgcca
     7861 ggcggtccag tcgcggctgc gggggaggtc ggggtcgcgc agctccaggg cggcgttgaa
     7921 gagcgagacc gccatggtca cgccgacgac gaggaggagc tggagaggga cctctctcct
     7981 ctggaggagc caggggacga gggcggtggc cagggcggcg agggctccca ggcccagggc
     8041 gccggcgatc cagctcatcc cctgccggcg ctccagacgg aagacgccgg tgtccagcga
     8101 gaaggccagg gcggcggcga tggcggcgag gtaggggccg tagaagaggg ggccccggcc
     8161 caggccattg gcggccgcca gcaccgcgaa ggcggggagg accgcgcgga ccaggatcct
     8221 caccttgagc cccgaggcat gacccggcgg cgcgaagcgg taccagagga ggacatagag
     8281 gccgtagccc acgagcgccg gcagcgccca gcgccagttg cccagcgacc aggcgccgta
     8341 ggcgaagagg atgaaggcga tcgctccgcc tacgttcagg gagtgcgagc gccagaccac
     8401 cagtgccacc agcaggcaga tcgcgaggag cgacaggttc tggaagatca cctcgcccaa
     8461 cggcttgctg gtgatcttcg ccaggatcag caccacggcc agggggatga agaggttgtc
     8521 cgccccctcc aggctgatcg cctcgaagcc ggtcaccagc gccgccacca gcagcgccgc
     8581 cagcacgcag accgggcgcg gcaggtcggt gagcagcagc gtcggcaggt ggatcgccag
     8641 gaaggcgagg accaggaaga ccagcgaccc ctccaggctc ttggtctcgt cctcgacctc
     8701 gtaacggacg cgcccgtact ggctgccgat cagcgccgcg aaggcgtccg ccaccgccag
     8761 caccaggacc gccgagaggt agagccacgg ccgcccctgg gtgaggacga aggtcaggaa
     8821 gaccgccagc gggtagtact ccgccccccg gctgcggcgc ttcaccccgt gcaggctctt
     8881 gaggaacccg agcttcgagc cggccacgaa cagtaccgtc agggggaggg ccatggccag
     8941 caccacccac ggggagcgca cccggaaggg gaagaggagg caggtgatgc ctcccccgag
     9001 gtggaccagc ttgcgcgtcc actcggcgtc cgggttgccg aagcggcgcc agagctcggc
     9061 gaagacgatc agggagagga agaccgccga gaggatcacc gctcccctca agtcttgcgc
     9121 ggtgatggtg atcacccggt cacctcgtcg acgacctcgt cgatcacgaa ggcgctgtag
     9181 aagaaggcga ggtcgcgggc gaagagctgc cctgcctcgt cgtccagggg ccggacgcgt
     9241 cccgggaagg agggggcgag ccgccgcggc gccagcatgt tccagtaggc gaagcgggcg
     9301 cccggacggg cggtcgccag cagccggccg tagacctggg cgctggtggc cgggtccatg
     9361 tactcgaaga tatccgacag gttgaagccg tcgaacccct cgccccgcgc cagccattcg
     9421 gcctcggcta ccggttcgac cgatccctcg aagagcgtca ggcggtccag gttctcccgc
     9481 accttcggga agacctccgg gcgcagccag tgggggaggg tatgcgtgaa gctgccggtg
     9541 aggatgtagt ccacgtaggg gttggcgtgc ggggggagca ccgtcagggc gtagcgggtc
     9601 cggccgagga tgcgctcggc gaccgacccc tcgacgtacc ggaagaactc ggggtcccgg
     9661 cccagcctcc ccatggtgaa gcggctgaag aagacccgga agaggagctt ccagcgccag
     9721 gtgtcccaga cccggccgta gaacttccgg cgcgccgcct cgtccttctc ctccagcagg
     9781 cgctcgaccg tccgccggcc gtggaccagg gggaggaccc ggcgccggaa gagatggaag
     9841 tagctctcga acttgccgtg gtggatgacg ccgccctcga tggcctcggg gcggtccccc
     9901 cagaactccc gggcctgatc ggagaggtgc ttcgccagat ggccgtagac cgcgaggcgg
     9961 ctcgcgctcg gccggacccc caggaaggtc agggccgttt cgtagtccag atgccggacc
    10021 gccgttttct tgagctccac caggttgagc tgagcggggg agaggtccac ggccaggacg
    10081 tgggcgccat gggagaggag ggagaagctg ttgtccccgg ccgacgcgat cgagaggatg
    10141 cgctggcccg gcgccggccg cagggcggcg cagagcacct gcgcgtcctc ccagacgttg
    10201 gcgtagcgga tcaggtcgaa gcgggcgcgc ttctcgatgc ggtcggccac ggtggcctct
    10261 accttatctc agcccagatc ggggctctcg agcaccgccc ggagcagccg ccgcaggtcg
    10321 atggccgcgg cctgggcggc ggcgaggacg tcggcgtggt ccaccgccgc ctcgctcatg
    10381 ccggcggcgg ggttgctcac catggagacg cagaggcagc gcatccccat gtgccgggcg
    10441 gcgatcacct cgaggaccgt ggacatgccg gtgacgtcgc cgccgagctg gcgcagcatc
    10501 cgcacctcgg ccggcgtttc gtagctcgga ccggcgactc cggcgtagat cccctcggtc
    10561 agggtgatgc cgagctccgc ggcggcggcg agggtcaggg cgcgcagctc caggtcgtag
    10621 gcggcgctca tgtcggggaa gcgcggcccc caggccgagg ggagctggcc gcggagcggg
    10681 ttgagccccg tcaggttgat gtggtcccgg atcagcacca ggtcccccgg cttctgcccg
    10741 ggctggaggc cgccggtggc gttggtcagg atcaggacct tggcgccgag cagggcggcg
    10801 aaacgcaccg ggaagaccgt gtcgtgggcg ctgtacccct ggtaggagtg gaggcggccg
    10861 cgctggtaga ggaccggcag gtccggccgc ggcagcagga cgtccagctc gtgggcgtgc
    10921 ccctcgacgg gatgcaggcc gaaggggagg aagtactcca gggagaccgg tccgtggatg
    10981 cggtcctcca tgtcgatgcc aagacccgag ccggtgacca tcgccacctt ggggcgcggc
    11041 cagcccaggt cgtcccagcg gtccaccgcc gccttgaggg gggtggtgag gtcgtcttcc
    11101 atctccaggt cgtggctcat ggcgaggcat cctagctcgt ccccggatac aatgcacccc
    11161 ggccggcggc ctagccggtc ttgtaagatc ttcaagtcac gagggccacg aggggaggag
    11221 cggcagcgca ccatgtccga gctcgacgag cagatccaga accggcgcga gaagcgccag
    11281 cgcctggcgg aggccggggt ccaggtctac ccccaccgct tcgagtggga cctggagccg
    11341 gccggcatcc acgcccgcta cggcgacaag tccgccgagg agctggacca ggccgccctc
    11401 cgcctgcgcg tcccgggccg ggtccgctcc atccgcgagc acggcaagac cgcgttcgtc
    11461 gacctccacg acggtaagga gaagctccag ctcttcatcc gcaaggaccg cctgtccgag
    11521 accggccgcc tggtcctcga aaacctcgac ctcggcgacc tggtgggcgc cgcaggcacc
    11581 ctgatgcgca cccgcatggg ggagctgtcg ctgtcgccag acgacctcac cctgctcgcc
    11641 aaggcggtcc ggccgatgcc cgagaagtgg catggcctgg ccgacatcga ggtccgctac
    11701 cgccagcgct atctcgacct cgccaccaac cccgagtcgc gccaggtctt cgagacccgc
    11761 gccgccatcg tgcggggcat ccgcgagttc ctcgaccgcc acggcttcct cgaggtcgag
    11821 acgccgatga tgcagatcct gcccggcggc gccacggccc ggccgttca
//
LOCUS       DASPJI010000522         8620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_203058, whole genome shotgun sequence.
ACCESSION   DASPJI010000522 DASPJI010000000
VERSION     DASPJI010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8620)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8620
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203058"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VEL74_14170"
     CDS             complement(<1..371)
                     /locus_tag="VEL74_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63723.1"
                     /translation="MAEGRRVRGSGVRFTLPYVLRVSGLWLAVSVVIFLVFSVACYLL
                     LLDRPTGPAQSNLMLLLGFQTIGVLAALVALAVFTTHRVAGPLIAIRRALDDVKAGDM
                     DRVLRLRGGDLHLRDIEGSFNG"
     gene            complement(413..1888)
                     /locus_tag="VEL74_14175"
     CDS             complement(413..1888)
                     /locus_tag="VEL74_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63724.1"
                     /translation="MIYSPSMNPAPPQARAGTLKALRYLALAGVLLLAARAGVTILRE
                     PCVGVADNLDYWRVARPAGIQVDPQPRQGSHVVCSYPLTTPDLKESLTSPSLLAWLVT
                     LPQRGTDGGSLDLRRIGTVYWLASVAVLAAAFFAGVNVLPLLMIAWVVYDPGFLLFFN
                     SLYADPALIVAQLATVLLLGLDPLDPRRVKALALLVGAAAVAGFSKMQYSPFPAVLLG
                     VCLLALVFHCRVPSRTQAGLLLVLLALAVAAPLHFVYGPAPRFLDANNYNATFGGIAR
                     VAADPAAALAALGIPEEHRGRKPKDYFAADSGPDDPVMPALRRLSRLRLLSLYLTDGT
                     ALSRTAQAVHADLGKVRTHPRGTFTRGEGGRPSRVFRTPEQFSLWRGRLLHRIPLWSL
                     LLLGGVAAFLAARAVRSGRGRWTAGETVLLFLVLWSTSQFAVAVLGEGFVNLHQHLLG
                     ARLGLDLLLVLVLCRLLLLVTRRDAPADAQVGQGSAKLPGL"
     gene            complement(2340..2738)
                     /locus_tag="VEL74_14180"
     CDS             complement(2340..2738)
                     /locus_tag="VEL74_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63725.1"
                     /translation="MATTKRQRTYTGSLGELRRALDAFNANASELGYLETQKNRIATL
                     LDRGLELARQQAALTASRQDVSKRLKITIEEARRSLSAVRATLKDHYGPRSEKLVEFG
                     IQPFRGRKPKPEEPSLEQNASAPPTDSSAI"
     gene            3204..3785
                     /locus_tag="VEL74_14185"
     CDS             3204..3785
                     /locus_tag="VEL74_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63726.1"
                     /translation="MRSFVQGSTSSVRPNHPNAFTASFLARLGERDEPPTAGDADVAG
                     PWRIEAVVWRGGSGFGLFRLGETLARGFAPAAVFPSRWLALLGAAILPGTGKDPLLLL
                     SKTPDADGAYPLALAGGGSGDGGDGGDGEDGPVGWFPLFEEKAVDGMNVLIHLLQSPE
                     SLSYLLEAAGAVALERCGAILDERLGREGAAAG"
     gene            4068..5903
                     /locus_tag="VEL74_14190"
     CDS             4068..5903
                     /locus_tag="VEL74_14190"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HYG63727.1"
                     /translation="MSLDIERVTRRPPARVPRPSPNGAAEAFWRTLQALEAEIQDIPH
                     SGGRHAQACAVTAATLEAFWARLQPRGARSWEIPRPVLDQPLPPLPGAASRLAAEAGV
                     IAAQQEPVTGGYMIGELYTALLPSELRARHGIFYTPPALVGRLLALASQAGTDWSVAH
                     VLDPACGGGAFLTPVALRMAAALRAEAPEAVFRHIVGHLRGFELDPFGAWMSQVLLEA
                     ALLDLCRSVKKRLPTVVTVCDALTTQPGERRFDLVIGNPPYGRVTLSPDLRETYRRSL
                     YGHANAYGLFMDLAVRWTRPGGTIAYVTPTGFLGGQYFQHLRALMAEEAPPVAVDLVT
                     SRRRVFSGVLQETLLAAYRRNGPRRKASVHVLSLTGEAAAEVVSAGTFSLPSKATDPW
                     LVPRSREQEVLISRLREMPHRLSDYGYKVSTGPLVWNRHKPQLRDATVAGAYPILWSE
                     CVTPDGRFEHRTEKKNHSPYLATNPGQEWLLTREPCVLVQRTTAKEQRRRLIAAELPV
                     RFIRKHRAVSVENHLNMVKPIVAAPPVPLRVIAAILNSEIADSVFRCISGSVAVSATE
                     LECLPLPPPKEARRLLDLLEAGAPSDAVEGRLRSLYLREKADAAA"
     gene            5890..6981
                     /locus_tag="VEL74_14195"
     CDS             5890..6981
                     /locus_tag="VEL74_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003187096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HYG63728.1"
                     /translation="MPLPDLLTIPEIRARLEAIFPEGTPRRSYFTRDIAANTVFVMLY
                     GGMIESMGRWLAPKHVYCMSDRQAEQQGEGERIAYNENVLKRGFKPAGQQWYKDNTRE
                     SIRDETLNGLVGVGAAILKSGVATNSGKPRYALQAGFAALFNPSLQEEGLSTAITSWR
                     EKHLDPLVLARMALIRQGAAAATADNVEVRLPNGSLRQLSSGLSSVISKAIIEVFAPA
                     FLYQPAIILLSESGNKIVAQEADLIARLGLRIDKATLLPDIILFDLAEGRELLVFVEV
                     VATDGPISESRRQALLTAAEGARILPARIAFVTAYWDRSHQAFRKSFESLAWNTLVWF
                     MTEPEHVVMLREKPELEKRRIFDLIREGG"
     gene            complement(7004..8251)
                     /locus_tag="VEL74_14200"
     CDS             complement(7004..8251)
                     /locus_tag="VEL74_14200"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HYG63729.1"
                     /translation="MNILVLNCGSSSLKFQVIATDLEAIERDADRELARGVVERVGSE
                     SLLTLRAEGRPARRLTAPLRDHRQALDHVLRWLVSPEASVPGVSSLGDVHAVGHRVVH
                     GGEKLRVSVLIDDAVIAQIEDTIDLAPLHNPANLRGITAARELFGPGIPQAAVFDTSF
                     HSTMPPESFLYALPYQLYVRHKVRRYGFHGTSHRYVAYRYRQLTGRPHEETRIITLHL
                     GNGCSACAILRGESLETSMGFTPVEGLVMGTRSGDLDPSVLEFLHHKEGMSFDELDTL
                     LNKRAGLLGISGLTNDMRELLAEEKENQDRRARLAIDIFCLRVKKYIGAYLARMNGAD
                     ALVFTGGIGENSPEIRRRICADLDFLGITVDPGRNDAITGGLEGEIGTDGARVRVYVI
                     PTNEELLIARDTIRCVLDAPRRW"
     gene            8250..>8620
                     /locus_tag="VEL74_14205"
     CDS             8250..>8620
                     /locus_tag="VEL74_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYG63730.1"
                     /translation="MEGSLLHRPASLGPEVDTLAAMLARRADETPETLAFAQVGGESL
                     GYGALRRDAEALAGGLAGLGVGPGDRVALLLPAGLDFIRAFFALQRLGAAPCALDPGA
                     PPATCARRAARVRPRLVLTSGS"
BASE COUNT         1330 a         2896 c         3109 g         1285 t
ORIGIN      
        1 ccgttgaagg agccctcgat gtcccgcagg tggaggtcgc cgccgcgcag gcggagcacc
       61 cggtccatgt ccccggcctt cacgtcgtcg agcgcccggc ggatcgcgat gagcggcccg
      121 gcgacccggt gggtggtgaa gaccgcgagg gccacgagcg ccgccaggac ccctatcgtc
      181 tggaacccca gcagcagcat caggttgctc tgcgcgggcc ccgtgggccg gtcgagcagc
      241 aggaggtaac aggcgacgct gaacaccagg aagatcacca ccgacaccgc cagccacaga
      301 cccgagacgc gcagcacata cggcagagta aaccggaccc cggacccgcg gacccgccgc
      361 ccttcggcca tgagagacct cctcgaagtt gtaaacgtga atccgaaaat ttctataagc
      421 cggggagttt agcagatccc tgcccgacct gagcgtcggc cggagcatcg cgtcgcgtca
      481 ccagaaggag gagccggcag agcaccagga cgagcagcag gtcgaggccg agccgcgccc
      541 ccaggaggtg ctggtgcagg ttgacgaacc cctcccccag gacggcgacg gcgaactggc
      601 tggtgctcca caggacgagg aacaggagca ccgtctcgcc ggcggtccag cggccccgtc
      661 ctgaacggac cgcgcgggcc gcgaggaacg ccgccacgcc gccgaggagg agcagggacc
      721 agagagggat ccggtggagc agacggccgc gccagagaga gaactgctcg ggcgtccgga
      781 agacccgaga cggccggccc ccttcccccc gggtgaacgt gccccgcggg tgcgtgcgga
      841 ccttgccgag gtcggcgtgc acggcctggg cggtgcggga gagcgccgtc ccgtccgtca
      901 ggtagaggct caggaggcgc aggcgcgaca gcctgcggag cgcgggcatc acgggatcgt
      961 ccggcccgct gtccgccgcg aagtagtcct tgggcttgcg gccccggtgc tcctccggga
     1021 tgccgagggc cgcgagcgcc gccgcgggat cggccgccac ccgcgcgatc cccccgaacg
     1081 tcgcgttgta gttgttggcg tccaggaacc ggggggccgg tccatacacg aagtgcagcg
     1141 gcgccgcgac ggccagggcc aggaggacga ggaggagccc cgcctgcgtc cggctcggca
     1201 cccggcaatg gaagaccagg gcgagcaggc agactccgag caggacggcc gggaacggcg
     1261 agtactgcat cttgctgaac ccggcgaccg ccgcggcgcc gaccaggagg gccagggcct
     1321 tgacccgccg cgggtcgagc ggatcgagcc ccaggaggag caccgtcgcc aactgggcga
     1381 cgatcagcgc cgggtccgcg tagagactgt tgaagaagag aaggaagccg gggtcgtaga
     1441 ctacccacgc gatcatcagg agcggcagga cgttcacccc cgcgaagaac gcggctgcca
     1501 ggaccgccac gctcgccagc cagtacacgg tcccgatccg ccgcaggtcg aggctgccgc
     1561 cgtcggtgcc ccgctgcggg agggtgacca gccaggcgag caggctgggg ctggtgagcg
     1621 attccttcag gtcgggcgtg gtgaggggat aggagcagac cacgtgggag ccctgccggg
     1681 gctgcgggtc cacctggatg ccggccggcc gcgccacccg ccagtaatcg aggttgtccg
     1741 ccacccccac gcacggctcg cgcaggatgg tcacgccggc gcgggccgcg aggaggagga
     1801 cgccggcgag ggccaggtag cggagggcct tcagggtccc cgcccgtgct tgcggcggcg
     1861 ccgggttcat ggagggggag tagatcacac gccctgcgcc gcggcggctc ggcgcccgtt
     1921 gcagcggcct gttgcagagt gcggcagggg attggaacga tacaacgacc tggcggagcg
     1981 agcggcaggc ttttgcaggg tgcgacggtg gattgcaacg cttcaatcgg ctggcgccac
     2041 ggacgacgag ccgttgaaga tcgcggcagc gcgtcgtaga tggcgacggc gcgccgcaag
     2101 gtgcggcaag gagatggaag gtgccggcgc gccgccggcg gtggcaatga atccttgaca
     2161 gatgcgccgg ctcgcaggac gttgcggtgg agcgttgccg gtgagcaacg gggcgtcgcg
     2221 gccttgcagt gcgccgccgt ccgcgccgcc agctcgtcgc gagttgcagc gccgcgccgc
     2281 gaggacaaaa aaagggggcg tgcggtgcac gccccctcgg tgaagcagcg atcaggagat
     2341 cagatggcgg aggagtccgt gggcggcgcg gaggcgttct gctcgaggct cggctcctcc
     2401 ggcttcggct tgcggccacg gaacggctgg atcccgaact cgaccagctt ctccgagcgc
     2461 gggccatagt ggtccttcag cgtggcccgt accgccgaca gcgagcgccg cgcctcctcg
     2521 atggtgatct tgagccgctt ggagacatcc tggcggctcg ctgtcagggc tgcctgctgg
     2581 cgggcgagct ccaagccccg gtccagcagg gtggcgatcc ggttcttctg ggtctccagg
     2641 tatcccaact cgctcgcgtt cgcgttgaac gcgtcgaggg cccgccgcag ctcccccagg
     2701 ctgcccgtgt acgtccgctg tctcttcgtc gtagccattg tcctattcct ttcttgaacg
     2761 agccggtccg gctcaggtcc ggcacctcca tggcgccgcg acgggcagag tatagatcat
     2821 gaagaccgat tcgggtcaat aggcataaat aggtctagct gtggtagatt caacttgccg
     2881 gcagtgaggc gaggctcttt gacaactgaa ggactcatcc gaggcgcggc gcgtgggcgg
     2941 cggagccgcg cctcgatgag ggggcacgaa gaaggcgaag aaagtcggcc cggacccctt
     3001 gactcctctc gcggcgagtc ataggatcgc gacatcagga cgtacccgga ggcgaattcc
     3061 gactccccca tccggtggaa tcgcgtgtgc ggggatgtcc tggggcagca gcggccggca
     3121 agggtgccgg ctggtggcgg cccggaaggg aggtgacgct cccgaccgga cctcgccaca
     3181 cacagtccta cttgggagga tctatgcgta gctttgtgca gggtagcacc tcgtccgtcc
     3241 gtccgaatca ccccaacgcc ttcaccgctt cgttccttgc ccgtctgggc gagcgggacg
     3301 agccgcccac ggccggcgac gccgacgtgg ccgggccgtg gcggatcgaa gctgttgtct
     3361 ggaggggggg ctcgggcttc ggcctgttcc ggttgggaga gacgctggcg cgcggcttcg
     3421 ctccggcggc ggtcttcccg tcccgctggc tggcgctcct gggagcggcc atcctgccgg
     3481 gcacggggaa ggacccgctc ctcctcctct ccaagacccc cgacgccgac ggcgcctatc
     3541 ccctcgccct cgcgggtggc ggcagcggcg acgggggcga cggcggagac ggagaggacg
     3601 gtccggtcgg ctggttcccc ctcttcgagg aaaaggcggt ggacggcatg aacgtgctga
     3661 tccacctcct ccagtcgccg gagtcgctct cctacctgct ggaggcggcc ggcgccgtcg
     3721 ccctggagcg gtgcggggcg atcctggatg aacggctcgg gcgggagggg gcggcggcgg
     3781 ggtagtcgct gagaatcacc ggcaggtcgg gcggggggaa acctgcccgg cctgtcggcc
     3841 cgctcttgcg catagctatt cttggaaatc ttgaagttga gcgattttct tgccgttttc
     3901 cgtctttgcc gggtatggca agcaaacttc ccttcattcc aggaggcgga gaccggttcg
     3961 cggtctctcg cttcgagccc ccggtacccg gcagcgggga tgctaccctt acgggtgttt
     4021 gccgggcacc catccaactc gggattccct tcggccttga ggagagcatg agcctggata
     4081 tcgagcgggt cacgaggagg ccgccggcgc gtgtcccccg tccgtcgccc aacggagcgg
     4141 ccgaggcttt ctggcgcacg ttgcaggcgc tcgaggccga gatccaggac atcccccact
     4201 ccggcggccg gcacgcccag gcctgcgcgg tcaccgcggc caccctggaa gccttttggg
     4261 cccggctcca acctcgtggc gccaggagct gggagatccc ccgcccggtc ctcgatcagc
     4321 ccctgcctcc cctgcctggg gcggcgagcc ggctggccgc ggaggcaggg gtcatcgcgg
     4381 cccaacagga gccggtgacg ggcgggtaca tgatcggcga gctctacacg gcgctcctgc
     4441 cgtccgagct tcgcgcgcgg cacggcatct tttatacgcc gccggcgctc gtcggccggc
     4501 tgctcgccct ggcctcccag gcgggcacgg actggtccgt ggcccacgtg ctggacccgg
     4561 cctgcggcgg gggcgccttc ctcaccccgg tggcgctccg gatggcggcg gcgctgcggg
     4621 ccgaggcgcc ggaggcggtc ttccggcaca tcgtgggcca cctccgcggc ttcgagctgg
     4681 accccttcgg cgcgtggatg tcgcaggtgc tcctcgaagc ggcgctgctc gacctctgcc
     4741 ggagcgtcaa gaagaggctg cccacggtcg tcacggtctg cgacgcgctc accacgcagc
     4801 ccggagagcg gcggttcgac ctggtcatcg gcaatcctcc gtatggacgc gtcacgctct
     4861 cgccggacct ccgggagacc taccggcgca gcctctacgg ccatgccaac gcctacggcc
     4921 tgttcatgga tctggcggtc cgctggaccc ggccgggcgg gaccatcgcc tatgtcaccc
     4981 cgacgggctt cctgggcggc cagtacttcc agcatctccg cgcgctgatg gccgaggagg
     5041 cgccgccggt ggccgtcgac ctggtcacca gccgtcgccg ggtcttctcc ggcgtcctgc
     5101 aggagaccct cctggcggcc taccggcgga acgggccgcg gaggaaggcc agcgtccatg
     5161 tcctgtcgct caccggagag gccgcggccg aggtcgtctc ggcggggacg ttctcgctgc
     5221 cgtccaaggc gaccgatccc tggctggtgc cccgcagccg cgagcaggag gtgctcatct
     5281 cacggctgcg ggagatgccc caccggctgt ccgactacgg gtacaaggtt tccacggggc
     5341 cactggtctg gaatcgccac aagccgcagc tccgcgacgc caccgtagcg ggtgcctacc
     5401 ccatcctctg gtccgagtgc gtgacgcccg acggacggtt cgagcaccgc acggagaaga
     5461 agaatcactc tccgtatctc gcgacgaacc cgggccagga gtggctgctg acgcgggagc
     5521 cgtgcgtcct ggtccagagg accacggcca aggagcagcg gcggaggctg atcgccgcgg
     5581 agctgccggt ccgcttcatc cggaaacacc gcgcggtctc ggtcgagaac cacctcaaca
     5641 tggtaaagcc catcgtggcg gcaccgccgg ttcccctgcg cgtcatcgcg gcgatcctca
     5701 acagcgagat cgcggacagc gtcttccggt gcatcagcgg cagcgtggcg gtgtcggcga
     5761 ccgagctgga atgcctgccg ttgccccctc cgaaggaggc gcggcgcctg ctcgacctgc
     5821 tggaggccgg ggcgccatcc gacgccgtcg aggggcgcct gcgctccctc tacctgcggg
     5881 agaaggccga tgccgctgcc tgacctgctg acgatcccag agatccgggc acggctggag
     5941 gcgatctttc cagaggggac gccccgccgc tcctacttca cgcgcgacat cgccgccaac
     6001 accgtcttcg tcatgctcta cggcggaatg atagagagta tgggccgatg gctcgctccg
     6061 aagcacgtct actgcatgag cgacaggcag gctgagcaac aaggggaagg agagcgtatc
     6121 gcttataacg aaaacgtcct gaaacgaggc ttcaagccag caggccagca atggtacaag
     6181 gacaacaccc gagagtcgat ccgggacgag accctgaatg gtttggtggg ggtcggcgcg
     6241 gccatcttga agtcgggggt cgccaccaac tcgggcaagc cacggtatgc actgcaggct
     6301 ggctttgccg ctctcttcaa tccctctctt caagaagaag gcctctccac ggcgatcacg
     6361 agctggcggg agaagcactt ggaccctctc gttctggccc ggatggctct gatccggcag
     6421 ggcgcggccg ccgccacggc tgacaacgtc gaggtgaggc tgcccaacgg ctctctccgg
     6481 cagctttcct ccgggctcag ctcggtcatc tccaaggcaa tcattgaggt gtttgccccg
     6541 gcgttcctgt atcagccggc catcatcctg ctcagcgaga gcgggaacaa gatcgtcgcc
     6601 caagaggcgg atctgattgc ccgcctcggc ctgaggatcg acaaggccac gctgttgccg
     6661 gacatcattc tcttcgactt ggcagagggc cgggagttgc tggtttttgt cgaggtggtc
     6721 gcgacagacg gcccgatttc ggagtcccgc cggcaggcgc tgctcacggc ggcggaggga
     6781 gcccgaatcc tgcccgcccg gatcgccttc gtcacggctt actgggaccg gagtcaccag
     6841 gccttccgga agagcttcga gtccctggcc tggaacacgc tggtctggtt catgaccgag
     6901 ccggagcacg tcgtcatgct gcgcgagaag ccggagctgg agaagagacg catcttcgac
     6961 ctgatccgcg agggcggctg agcgccgccc atggtccgcg ccttcaccag cgccgcggcg
     7021 cgtccagcac gcagcggatg gtgtcgcggg cgatgaggag ctcctcgttg gtggggatga
     7081 cgtagacgcg cacccgcgcc ccgtcggtac cgatctcccc ctccaggccg ccggtgatgg
     7141 cgtcgttgcg gccgggatcg acggtgatcc ccaggaaatc gaggtcggcg cagatccggc
     7201 gccggatctc gggggagttc tcgccgatgc cgccggtgaa gacgagggcg tcggcgccgt
     7261 tcatccgcgc caggtaggcg ccgatgtact tcttcacccg cagacagaag atgtcgatgg
     7321 cgaggcgggc gcggcggtcc tggttctcct tctcctcggc gagcagctcg cgcatgtcgt
     7381 tggtcaggcc cgagatgccc aggaggccgg cgcgcttgtt gagcagggtg tccaactcgt
     7441 cgaagctcat cccctccttg tgatggagga actcgagcac cgacgggtcg aggtcgccgc
     7501 tccgcgtccc catgaccagc ccttccaccg gggtgaaccc catggaggtc tcgagcgact
     7561 cgccgcgcag gatggcgcag gccgagcagc cgttgcccag gtgcagggtg atgatgcggg
     7621 tctcctcgtg cggccggccg gtgagctggc ggtagcgata ggccacgtag cggtgcgagg
     7681 tgccgtggaa gccgtagcgg cgcaccttgt ggcggacgta gagttggtag gggagggcgt
     7741 agaggaacga ctccggcggc atggtggagt ggaacgaggt gtcgaacacg gccgcctgcg
     7801 ggatccccgg gccgaacagc tcgcgcgccg cggtgatccc ccgcaggttc gccgggttgt
     7861 gcagcggcgc caggtcgatg gtgtcttcga tctgcgcgat gacggcgtcg tcgatcagga
     7921 cggagacgcg gagcttctcg ccgccgtgga ccacccggtg gcccaccgcg tgcacgtccc
     7981 ccagggagct gacgccgggg accgacgcct ccggcgagac cagccagcgc aggacatgat
     8041 ccagggcttg acggtgatcg cgcagcggcg cggtcaggcg gcgcgccggc cgcccttcgg
     8101 cacgcagggt gagcagcgac tcgctgccga cccgctcgac gaccccgcgg gccagctcgc
     8161 ggtccgcgtc gcgctcgatg gcctccaggt cggtggcgat cacctggaac ttgagagagg
     8221 agctgccgca gttgaggacg aggatgttca tggaaggaag cctactgcac cgcccggcct
     8281 ctctggggcc cgaagtggac accctcgccg ccatgctggc ccgccgggcg gacgagaccc
     8341 ccgagaccct ggccttcgct caggtcgggg gagagagcct gggctacggg gcgctgcgga
     8401 gggacgcgga ggcgctggcg ggcgggctgg ccggcctggg cgtcgggccg ggggaccggg
     8461 tcgcgctcct cctgccggcc gggctggact tcatccgcgc gttcttcgct ctccagcgcc
     8521 tgggggcggc cccctgcgcg ctggacccgg gagcgccgcc ggcgacgtgc gcccgccgcg
     8581 cggcgcgggt gcgtccgcga ctggttctga ccagcgggtc
//
LOCUS       DASPJI010000523         4661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_261156, whole genome shotgun sequence.
ACCESSION   DASPJI010000523 DASPJI010000000
VERSION     DASPJI010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261156"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VEL74_14210"
     CDS             <1..688
                     /locus_tag="VEL74_14210"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HYG63731.1"
                     /translation="PQPAAAVPGAAPAPPPVDIAGMVDQQLAQREEELRKQLEAQQAK
                     LEKELAKAKAAGQPTPQPMPQATAAPAQPAPAAEPSPSPAAAAATVVPPPAPSPSPEP
                     EPPAPTPQQAQQAAAPEPSPEPERAPSPVAPVAEAGDVVPPQLVSSPKPQYPPVARRM
                     RVEGTVVVSVLVDEQGQVQDARVTQPVSQKVGINEEALKVARAARFRPATKNGVRVKM
                     WARLTIPFKL"
     gene            765..1001
                     /locus_tag="VEL74_14215"
     CDS             765..1001
                     /locus_tag="VEL74_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63732.1"
                     /translation="MSLTEQQQKFYENILSVSKQEMDELDRMIEEELAKVKDRLAELQ
                     NAKKASRQMYDAACMRLGVPNDLDAEEGGGLAEA"
     gene            1095..1952
                     /gene="accD"
                     /locus_tag="VEL74_14220"
     CDS             1095..1952
                     /gene="accD"
                     /locus_tag="VEL74_14220"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HYG63733.1"
                     /translation="MAWFRKTKKPKPIRSERPRSNVPEGLWVKCEGCKEVIYSRDLDR
                     NLKICPKCGYHFRIDARTRIALLLDEPTPRELFTNVSPSDPLSFRDTKRYRDRVKTYQ
                     QMVGERDAVIVVQGRLEDIPVMLAVMEYRFMGGSMGSVVGEKITRAAERALERRSPLI
                     VVSASGGARMQEGVLSLMQMAKISAALARLREARLPYISVLTDPTTGGVTASFAMLGD
                     LNIAEPGALIGFAGPRVIEQTIRQSLPEGFQRSEFLVEHGFLDLVVKRSEMKETLARC
                     LRHLTPPRP"
     gene            1970..3427
                     /locus_tag="VEL74_14225"
     CDS             1970..3427
                     /locus_tag="VEL74_14225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HYG63734.1"
                     /translation="MPRTADSILSRLETLGIRLGLDNVRAVLAALGDPQRRFPSILVA
                     GSNGKGSTSALLAAMASAAGYRTGLYTSPHLETVDERLRIDGRPVPSGRLGDLLAEAV
                     EAAERRTGAPPTYFEALTVAAFRWFAAEQVDLAVVEVGMGGRLDATNTCEPLLSLITS
                     ISLEHREHLGDTLAAIAGEKAGILRRGRPALMWIDEPEAADAVRAAAHTLGTDLRSAP
                     DLVHLDGTESLGWDGQRVRLTTPETTHDLRLALPGPHQIRNLGLAVLAAETLAGLGFG
                     RLDARAIADGATACRWPGRLEPVELPGGRRVLLDAAHNAEGAAAFGTFLSTLATPVDL
                     LFGALGDKDIASMLAALAPRARRLILTAPPSPRAVAPHDLLRHLPVPEGGRVGVRGTG
                     AGDWQRGRGEGGSAHEGAEHGPPDPNPEAAREPIVEPDPDRALDAALASTDGVLTDHD
                     GVLAVCGSIFLVGDIRRRLRERFGVPPPAAYISGS"
     gene            complement(3409..4644)
                     /gene="glyA"
                     /locus_tag="VEL74_14230"
     CDS             complement(3409..4644)
                     /gene="glyA"
                     /locus_tag="VEL74_14230"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HYG63735.1"
                     /translation="MKAADPEIYQAIADERNRQNFGLELIASENFVSQAVMEAAGSVL
                     TNKYAEGYPGRRYYGGCEFVDVAERLAIERAKRLFGAEHVNVQPHSGTSANMAVYFGF
                     LRPGDKLMGMDLSCGGHLTHGHRLSYSGRDFQVVAYGVDRESERIDYDAVERQAMEER
                     PKLIVCGASAYSRVIDFERFRAIADKAGALLLADIAHIAGLVAAGLHPSPVPHCHFVT
                     TTTHKTLRGPRAGLVMCKEEFAKDVDRAVFPGLQGGPLMHIIAAKAVALHEAQQPEFK
                     EYQRQIVANAARLAKRLVDLGLRVVSGGTDNHVFLLDVGAAGLTGKVVEKALDASAIT
                     VNKNTIPFDPNPPLVASGIRLGTPALTTRGMREPEMDIVGDLIAEVLKAPEDTATQER
                     VRRQVRELCERFPLYDPLM"
BASE COUNT          674 a         1750 c         1594 g          643 t
ORIGIN      
        1 gccccagccg gccgcggccg tgcccggagc ggcgccggca ccgcccccgg tggacatcgc
       61 cggcatggtg gaccagcagc tcgcccagcg cgaggaggag ctgcgcaagc agctcgaggc
      121 gcagcaggcg aagctcgaga aggagctggc gaaggccaag gcggcgggcc agcccacgcc
      181 gcagcccatg ccccaggcta cggcggcgcc cgcgcagccg gccccggccg cggagccctc
      241 cccgtcgcct gccgccgcgg ccgccaccgt ggtgccgccg ccggccccct ctccttctcc
      301 cgagcccgag ccccccgcgc cgacgccgca gcaggcgcag caggccgccg ctcccgagcc
      361 gtcgccggag ccggagcgcg ccccctcgcc ggtggcgccg gtggccgagg cgggcgatgt
      421 ggtgccgccg cagcttgtca gctccccgaa accgcagtat cctcccgtcg ccagacgcat
      481 gcgggtcgaa gggacggtcg tcgtctccgt cctggtggac gagcagggac aagtccaaga
      541 cgcgcgggtg acgcaaccgg tctcccagaa ggtgggcatc aacgaagagg cattgaaggt
      601 ggcccgggcg gcccgcttcc ggcccgcgac caagaatgga gtccgggtca agatgtgggc
      661 ccgcctgacg atcccgttca agctttgatg ctggcttcca gccgcgaccc tgaaaggcgc
      721 ggctcccggc cgtctcgacc gggggggatt tttcggagga gacgatgagc ctgaccgagc
      781 agcagcagaa gttctacgag aacatcctga gcgtctccaa acaggagatg gacgagctcg
      841 atcgcatgat cgaggaggag ctggccaagg tgaaggaccg cctcgccgag ctccagaacg
      901 ccaagaaggc ctcccggcag atgtacgatg ccgcctgcat gcggctcggc gtcccgaacg
      961 atctggatgc cgaggagggc ggcgggctcg ccgaggcctg aggcgttctc gcctctcccc
     1021 gacgttcccg cgcagctccg aacgaaccgg gccccctccg gggcccgccg gctgacatcg
     1081 gctgaacatc tcctatggcc tggttccgca agaccaagaa accgaagccc atccgcagcg
     1141 agcggccgcg cagcaatgtg ccggaggggc tgtgggtcaa gtgcgagggg tgcaaggagg
     1201 tcatctactc ccgggacctc gaccgcaacc tcaagatctg cccgaagtgc ggctaccact
     1261 tccggatcga cgcccgcacc cgcatcgccc tgctcctcga cgagcctacg ccgcgtgagc
     1321 tgttcaccaa tgtgagcccg agcgacccgc tcagcttccg ggacaccaag cgctaccgcg
     1381 accgcgtcaa gacctaccag cagatggtgg gcgagcgcga cgcggtcatc gtcgtccagg
     1441 ggcggctcga ggacatcccg gtgatgctcg ccgtcatgga gtaccgtttc atgggcggct
     1501 ccatggggtc ggtggtgggc gagaagatca cccgcgcggc ggagcgggcg ctggagcggc
     1561 gcagcccgct tatcgtcgtc tcggcctcgg gcggcgcccg gatgcaggag ggggtcctgt
     1621 cgctgatgca gatggcgaag atctccgccg ccctcgcccg cctgcgcgag gcccgactcc
     1681 cctacatctc ggtgctgacc gatcccacga ccggcggcgt caccgcctcc ttcgccatgc
     1741 tgggcgacct caacatcgcc gagcccgggg cgctgatcgg tttcgcgggc ccccgcgtca
     1801 tcgagcagac gatccgccag agcctgcccg aggggttcca gcgcagcgag ttcctcgtcg
     1861 agcacggctt cctcgacctg gtggtcaagc gctccgagat gaaggagacg ctcgcccgct
     1921 gcctgcggca cctgacgcct ccccgcccct gagagcaggg ggcgtcgccg tgccgcgcac
     1981 cgccgattcc atcctttccc gtctggagac cctgggcatc cgcctggggc tggacaacgt
     2041 ccgtgccgtg ctggcggcgc tgggcgaccc gcaacgccgc ttcccctcga tcctggtggc
     2101 ggggagcaac ggcaaggggt cgacctcggc gctcctcgcc gccatggcct ccgccgccgg
     2161 ctaccgcacc ggcctctaca cctcgccgca cctcgagacg gtggacgagc gcctgcggat
     2221 cgacggccgc cccgtcccct ccggccggct gggagacctg ctggccgaag ccgtcgaggc
     2281 cgccgagcgg cggaccggcg ctccgcccac ctacttcgag gcgctgaccg tcgccgcctt
     2341 ccgttggttc gccgcggagc aggtcgacct cgcggtcgtc gaggtgggga tgggcggccg
     2401 gctcgacgcc accaacacct gcgagccgct gctctccctc atcacctcga tcagcctgga
     2461 gcaccgcgag cacctggggg acaccctggc cgccatcgcc ggagagaagg cgggcatcct
     2521 gcgccgcggc cggccggccc tcatgtggat cgacgagccc gaagcggcgg acgccgtccg
     2581 cgccgccgct cacaccctgg gcactgacct ccgttccgcg ccggacctgg tccacctcga
     2641 cggcaccgaa tccctcggct gggacggcca gagggtccgg ctgaccaccc ccgaaaccac
     2701 ccacgacctg cggctcgccc tgcccggccc ccatcagatc cgcaacctcg ggctggccgt
     2761 cctggcggcc gagaccctgg ccggcctggg cttcggccgt ctggacgccc gcgccatcgc
     2821 cgacggcgcc accgcctgcc gctggccggg ccgactcgag ccggtcgagc tccccggcgg
     2881 ccgccgcgtc ctcctcgacg ccgcccacaa cgccgaagga gccgccgcct tcggcacctt
     2941 cctctcgacc ctcgccaccc ccgtcgacct cctcttcgga gccctggggg acaaagacat
     3001 cgcctccatg ctcgccgccc tggctccccg agcccggcgc ctcatcctca ccgctccacc
     3061 cagtccccgg gccgtcgccc cccatgacct cctccgccat cttccggtcc cggaaggggg
     3121 gagggttggg gtccggggga ccggcgcagg cgactggcag cgtgggaggg gggaaggggg
     3181 gagcgcacac gaaggagccg agcacggtcc cccggacccc aacccggagg cggcccgcga
     3241 gccgatcgtc gagccggacc ccgaccgcgc cctggacgca gccctcgcct ccaccgacgg
     3301 cgtcctcact gaccatgacg gcgtcctcgc cgtctgcggc tccatcttcc tggtcgggga
     3361 tatccggcgg cggctgcgcg agcgcttcgg cgtgccgccg ccggcggcct acatcagcgg
     3421 atcgtagagc gggaagcgct cacacagctc ccgcacctga cggcggaccc gctcctgcgt
     3481 ggcggtgtcc tccggcgctt tcagcacctc ggcgatcagg tcccccacga tgtccatctc
     3541 gggctcccgc atgccgcggg tggtgagggc cggcgtgccg agacggatgc ccgaggcgac
     3601 cagcggcggg ttcgggtcaa aggggatggt gttcttgttg accgtgatgg cggaggcgtc
     3661 cagcgccttc tccaccacct tgccggtgag gccggcggcg cccacatcca gcaggaacac
     3721 atggttgtcc gtgccccccg acacgaccct gagcccgagg tccaccagcc gcttggccag
     3781 ccgcgccgcg ttggccacga tctgccgctg gtactccttg aactcgggct gctgcgcctc
     3841 gtgcagcgcc accgccttgg ccgcgatgat gtgcatcagc ggaccgccct ggaggccggg
     3901 gaagacggcg cggtccacgt ccttggcgaa ctcctccttg cacatcacca gaccggcccg
     3961 gggaccgcgc agggtcttgt gggtggtggt ggtgacgaag tggcagtgcg gcaccggcga
     4021 cgggtggagg ccggcggcca ccaggcccgc gatgtgggcg atgtcggcca acagcagggc
     4081 gccggccttg tcggcgatcg cccggaagcg ctcgaagtcg atgacccggc tgtaggcgct
     4141 ggcgccgcag acgatcagct tcggccgctc ctccatcgcc tgccgctcga cggcgtcgta
     4201 gtcgatccgc tcgctctcgc ggtcgacgcc gtaggccacc acctggaagt cacggcccga
     4261 gtaggacagg cggtgcccgt gcgtgaggtg accgccgcag gagaggtcca tccccatcag
     4321 cttgtcgccc ggccgcagga agccgaagta gacggccatg ttggccgagg tgccgctgtg
     4381 gggctggacg ttgacgtgct cggcgccgaa caggcgcttg gcgcgctcga tggccagccg
     4441 ctcggcgacg tcgacgaact cgcagccgcc gtagtagcgg cggccgggat agccctcggc
     4501 gtacttgttg gtgaggacgc tgccggcggc ctccatcacc gcctggctga cgaagttctc
     4561 ggacgcgatc agctcgagcc cgaagttctg ccggttccgc tcgtcggcga tggcctggta
     4621 gatctcgggg tcggcggctt tcagcgaacg gtaatcgagc a
//
LOCUS       DASPJI010000524         3003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_261206, whole genome shotgun sequence.
ACCESSION   DASPJI010000524 DASPJI010000000
VERSION     DASPJI010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261206"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2579
                     /locus_tag="VEL74_14235"
     CDS             <1..2579
                     /locus_tag="VEL74_14235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ELWxxDGT repeat protein"
                     /protein_id="HYG63736.1"
                     /translation="TGSNARPLGAVDGVLLFAADDGASGRELWKTDGTAAGTALLTDL
                     EPGAGGSGPEALAVIGGQLFFSAMTSAHGSELWVSDGTAAGTFMVADIFPGAGHALAG
                     GPPVPANSFVAIAAGKLFFAANDGSHGSELWMSDGTEAGTTLVQDIYPGTTGSGPSEP
                     VAMGGQVYFVAADDTHGRELWKTDGTGTVLVKDVAAGAADGHSPFGFSALTALGSQLL
                     FFAQDGTHGTELWTSDGTEAGTALLADLQPGPGSSWFGEIIGFDFRIVSGGRWYFRVY
                     DGSPFEGREVWVSDGTPAGTHLLKEINAQTSAFDVLWNGTLWGRALADAGGALFFRGN
                     GGSTGGELCKSDGTPAGTGVVADLWPGEGTSSVQGLTGLGGTVLFSADDNANGSELWA
                     SDGTAAGTELIADLQAPITGSPDDYPRQLVRLGSSVLFAGTGGLWKTDGTAAGTEQLY
                     YELDIGVPAVLGSEAFFSGLVSGIDGELWKSDGTAGGTLLVRDIRPGTDSSAPDGLVV
                     AGSLLFFSADDGSAGRELWKSDGTEDGTVVVKDIVPGAGSSILMSSSYPEGGEIALAA
                     AGSTVFFLASDGVAGEELWKSDGTEAGTVLVKDIFPGPRSSEIRWLTGVGSQGNRVFF
                     VADDGVHGRELWVSDGTAAGTVLVEDILPGAGSSLPAELSAHEDVLLFSAHDGAHGRE
                     PWRSDGTATGTRLVQDIAPGPRPSTPLRFTRSGSRVYFAATDAATGFELWALPVAQLT
                     GPGFYTVTPCRAVDTRTGAPLASGVNRTFMVAGSCGIPADATAIAANLTVLTPAGGGF
                     VVAWPAGTPLPGTSNVNFSAGQTRAASSILSLSATGLAARATLAGGGTVHLLVDVVGY
                     FK"
BASE COUNT          416 a         1114 c         1029 g          444 t
ORIGIN      
        1 tcactggttc gaacgcccgt cccctcggcg cggtcgacgg cgtgctcctc ttcgcggcgg
       61 acgacggcgc ctccggccgc gagctgtgga aaaccgacgg caccgccgcc ggcaccgccc
      121 tcctcaccga tctggagccg ggagccggcg gctccggccc cgaggcgctg gcggtcattg
      181 gcggacagct cttcttcagc gccatgacca gcgctcacgg cagcgagctc tgggtttccg
      241 acggcacggc cgccggcacc ttcatggtgg cggacatctt cccgggtgcc ggccacgctc
      301 tggccggcgg gccgcccgtc cccgccaaca gcttcgtcgc catagccgcg ggcaagctct
      361 tcttcgccgc gaacgacggc tcccacggca gcgagctctg gatgagcgac ggcaccgaag
      421 ccggcaccac cctggtccag gacatctacc cgggaaccac cggcagcggc ccgagcgaac
      481 cggtggcgat gggcgggcag gtctacttcg tggccgcgga cgacactcac ggccgggagc
      541 tgtggaagac cgacggcacc ggcaccgtcc tggtgaagga cgtggcggca ggggccgccg
      601 acggccactc ccccttcggc tttagcgcgc tcacggcatt gggttcccag ttgctgttct
      661 tcgcccagga cggcacccac ggcaccgagc tgtggacgag cgacggcacc gaggccggca
      721 ccgcgctgct ggccgacctc cagccgggcc ccggctcctc ctggttcggc gagatcatcg
      781 gcttcgattt caggatcgtc tccggcggtc gctggtactt ccgggtctac gacggatctc
      841 ccttcgaggg gcgggaggtc tgggtctcgg acggcacccc tgccggcacc cacctgctga
      901 aagagatcaa cgcccagacg tccgccttcg atgtgctctg gaacggcacg ctctggggcc
      961 gggcgctggc cgatgccggc ggggcgctgt tcttccgggg gaacggtggc agcaccggcg
     1021 gcgagctgtg caagagcgac ggcacccccg ccggcaccgg cgtggtggcc gacctctggc
     1081 ccggggaggg gacgtcgtcc gtccaggggc tgaccggcct cggcggcacg gtcctcttct
     1141 ccgccgacga caacgccaac ggcagcgagc tgtgggcgag cgacggcacc gccgccggca
     1201 ccgagctgat cgccgacctc caggccccga tcaccggctc gcccgacgac tacccgcggc
     1261 agctcgtgcg tctgggcagc tccgtgctct tcgccggcac cggcggcttg tggaaaaccg
     1321 acggcaccgc ggccggcacc gagcagctct attacgagct cgacatcggc gtgccggccg
     1381 ttctggggtc cgaagccttc ttcagcggcc tcgtctccgg aatcgacggc gagctctgga
     1441 agagcgacgg caccgccggc ggcaccctcc tggtgaggga catccggccg gggaccgact
     1501 cctcggcacc ggatggcctg gtggtcgcgg ggtctctgct cttcttctcc gccgacgacg
     1561 gcagcgccgg ccgcgagctg tggaagagcg acggcaccga ggacggcacg gtcgtggtga
     1621 aggacatcgt gcccggcgcc ggctcgtcca tcctcatgag ctccagctat ccggagggag
     1681 gagagatcgc cctggcggcc gccggctcca cggtgttttt cctggcgagc gacggcgtgg
     1741 cgggcgagga gctgtggaag agcgacggca ccgaggccgg caccgtcctg gtcaaggaca
     1801 tcttccccgg cccgcgctcc tccgagatcc gctggctgac aggcgtcggg agccagggaa
     1861 accgcgtctt cttcgtcgct gacgacggcg tccacggccg cgagctgtgg gtgagcgacg
     1921 gcacggcggc gggcacggtg ctggtcgagg acatcctccc cggcgccggc tcgtcgctgc
     1981 cggcggagct gtcggcccac gaggacgtcc tcctcttctc ggcccacgac ggcgcccatg
     2041 gccgcgagcc ctggcgtagc gacggaaccg ccacgggcac ccggctcgtc caggacatcg
     2101 cgcccggccc gcgcccctcg acccctctgc gcttcacccg ctcgggctcc cgcgtctact
     2161 tcgccgccac cgacgccgcg accggcttcg agctctgggc tctgccggtg gcccagctca
     2221 cgggccccgg cttctatacc gtcaccccct gccgggcggt cgacacccgg acgggcgcgc
     2281 cgctcgcctc gggcgtcaac cgcacgttca tggtggcggg ctcctgcggc atcccggccg
     2341 acgccacggc gatcgcggcg aacctcaccg tgctgacacc ggcgggcggc ggcttcgtgg
     2401 tggcctggcc cgcgggcacg ccgctccccg gcacgagcaa cgtcaacttc agcgccggcc
     2461 agacgcgcgc cgccagctcc atcctctccc tgtccgccac cggcctggcg gcccgtgcca
     2521 ccctggccgg cggcggcacg gtgcatctgc tcgtggatgt ggtgggctac ttcaagtgag
     2581 gctcaccggc aggagggcca ggcggcgggc cgtttgaaat ctatcgattg tcaaagaacc
     2641 tccgcggtcc gccccgcctc ggacctcctc cgccccgcgg gtcgtgaggg tacgcgggtt
     2701 tcgcgtcccg gccgccccga gtcccacctc tcgtgacccg gcgagcccat cgctcgaccc
     2761 tcgtccgctc tgccgcgacc cccgggtcga gagcctggac ccaagggtcg tgactaccgc
     2821 ggctcgggtc cgacgaagtg acccgagggt caagaacctt caagctcggg tccaattgaa
     2881 gacgagaagc caaatgatgg ttctataaga ccgtatcgag ccaaggaagt caatttcggt
     2941 cttgcatccg gccgggaccc catgtagagt ggctggcgca ccgttgattg aggagcctga
     3001 atg
//
LOCUS       DASPJI010000525         4745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_779593, whole genome shotgun sequence.
ACCESSION   DASPJI010000525 DASPJI010000000
VERSION     DASPJI010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779593"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VEL74_14240"
     CDS             <1..817
                     /locus_tag="VEL74_14240"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HYG63737.1"
                     /translation="AHEVAHQWWGQQMVGANVKGVGMLHETLAQYSALMVTEHELGRQ
                     RMRRVLQYELDWYLRGRAGERGVEPPLALVERQDYVYYHKGALAMYALRDAAGEERLN
                     RALSRYLSRVAFQGPPYTTAAELLAEVRQVVPEGSKHLVEDLFERITTFDNRLAAATS
                     TERADGTFLVRLELEAHKLRGDGTGAEREIPIDDWIDIAVFGEGEEGEGGGTPLFLER
                     RRIRASPVALEIVVGGRPAWVAIDPYYKLIDRNRGDNARAVLSAGAQTPSALR"
     gene            complement(880..1371)
                     /locus_tag="VEL74_14245"
     CDS             complement(880..1371)
                     /locus_tag="VEL74_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63738.1"
                     /translation="MTPNAFDADFLKRLEEQEEPPTASEADLAGPWHVEPLPEEEGGG
                     FGLFRAGESRERSFRPFARFESSWAAQLVAALLPGLARDAAYRFREERDADGWAIESR
                     ECWGAVIGHAAVFNPALIDALNLVDGLMRSPLALAGFLEACGQVALEKAGVILAERVG
                     GEG"
     gene            complement(1748..4516)
                     /locus_tag="VEL74_14250"
     CDS             complement(1748..4516)
                     /locus_tag="VEL74_14250"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HYG63739.1"
                     /translation="MPRPLALPPLRNWQDFEDLCLDLWRRLWNDPDAQKHGRQGQPQA
                     GVDVYGRPEGGREWAGVQCKRLEENLTPKVIEEEVEKARTFEPALSRFIIATTARSDV
                     HCQEAARKITDAGFPVTVVSWDEICHHLEDHPDLLRKYFPALIDPAPTGYRCMAIRPE
                     GFVEREELDQIRDILCGSGASAVGITSALRGAGGFGKTTLAQALCHDDRVRAAYPDAI
                     LWTTIGDQASEADRLSRVLDLIRFWTPGDLPHFDTVEAAAARLREILAGRRVLVVVDD
                     AWSPADVQPFRGAGTGLLVTTRLRETLPDGCACVDVDAMRLDEAVKLLTAELPVAEGL
                     RSRLEDLARTLGEWPILLKLVNRTLRDLVEDGLGVEKALDEIGEGLDAAGLEAFDQKY
                     SSEREKAVSRTLEVSLRRLSEDEQRRFEALAIFPEDEDVPLQIVERLWSCSAFEAKQL
                     ARRLHRLSLLLSLDLDAGTLRLHDVFRKYLIGRQGDAIAALHQDFLARSRSASGRWAD
                     LPLSESYLWRWLPYHLVHGGHSDILRELLLNFRFLAARLQATNVIALIGGFDYLLHDP
                     DLRIVRSVIYRSVLTLILYPEQLAGQLVGRLPEGDIWGWERLMQEARAAGKERLLLSG
                     GSGLLPRRASLLPAAFLRSEHTGSVCSVTILEKGQALSGATTGPLCLWDVKTGACLRT
                     FNGHTGIYALTAVGGRLALSGSRDGSLRLWNVRTGACLRQFEGQAGANVLAMVGEGNA
                     ILGSDGMLCLWNAKTGACLRTFEGNTEVCALAAVGERRALSGSRDGTVRLWDVDTGAC
                     LRTFERHLLPVTAVTAVGRGRGLSGSLDLTVRLWELETGACLRIFFGHTGSVNALAAV
                     GEDQALSGSGDGTLRLWDLGTGACLAVFTFDHSVTSVAVLNRVVIVGDEGGNVHFLDL
                     ILPGSDLI"
BASE COUNT          849 a         1612 c         1540 g          744 t
ORIGIN      
        1 ggcgcacgag gtcgcgcacc agtggtgggg ccagcagatg gtcggcgcca acgtgaaggg
       61 tgtgggcatg ctccacgaga cgctggcgca gtactcggcg ctgatggtca ccgagcacga
      121 gctcggccgg cagcggatgc gccgcgttct ccaatacgag ctggactggt acctccgcgg
      181 ccgcgccggc gagcgcggcg tggagccgcc tctcgcgctc gtcgagcggc aggactacgt
      241 ctattaccac aagggcgcac tggcgatgta tgccctccgc gatgccgcgg gtgaggagcg
      301 gctcaaccgt gcgctgtcgc ggtacctctc gagggtcgcc ttccaggggc cgccctacac
      361 cactgcagcc gagctcctcg cggaggtccg ccaagtggtg cccgaggggt cgaagcatct
      421 ggtcgaggac ctgttcgaga ggatcacgac gttcgacaac aggctcgccg ccgcgacctc
      481 caccgagcgc gcggatggca cgttcctcgt ccgcctcgag ctggaggccc acaagctgcg
      541 gggcgacggc acaggcgccg agcgggagat cccgatcgac gattggatcg acatcgcggt
      601 cttcggcgag ggagaggaag gtgagggcgg cggcaccccg ctgttcctcg agcggcgacg
      661 tatccgtgcc tcgccggtcg ccctcgagat cgtcgtcggc gggcggcccg cgtgggtggc
      721 gatcgacccg tactacaagc tcatcgaccg caaccgcggc gacaacgcgc gcgccgtgtt
      781 gtcggcgggc gcacaaactc catctgccct tagatgacct cgtctgaagc cagacggccc
      841 ggatcactcc gggccgtcct cttctctatc ttctacttcc tacccttccc cgcccacccg
      901 ctccgccagg atcacccccg ccttctccag cgccacctgg ccgcaggcct ccaggaagcc
      961 cgcgagcgcc aggggggagc gcatcagtcc gtcgacgagg ttgagcgcgt cgatcagggc
     1021 ggggttgaag accgcggcgt ggccgatgac cgcgccccag cactcgcggc tctcgatggc
     1081 ccagccatcg gcatcccgct cctcccggaa ccggtatgcg gcatcccgcg ccagaccggg
     1141 aaggagcgcg gcgacgagtt gcgccgccca ggaggactcg aagcgggcga agggccggaa
     1201 gctccgctcc cggctctcgc cggcacggaa gagcccgaag cctccaccct cctcctccgg
     1261 cagcggctcg acatgccagg ggccggcgag atccgcctcc gaggcggtgg gcggctcctc
     1321 ctgctcctct agacgcttca agaagtccgc gtcgaaggcg ttgggggtca cctcgtccgg
     1381 atccggcgcc gcctcatcag gcgccttctc aggcgaaaac ggcgatgacg acgtgggggg
     1441 aacgacgttg ttaccatcct ttgtactgcg cattggggtg ctcactcctt tgcgtagagg
     1501 tccggggtcg agcgtccaac tcttccccgg gccgctttct aggtggtccc ggccggctgg
     1561 cgcgaagaag ctatcgccgg ccggggtctt cccttgtcaa tgaacgcgtc cgctcgaacg
     1621 gcaacggatg gtgagaggat agcggaacgt ccgcaaggac gtcaaggggt ttttcgaggg
     1681 aacgggagag cgtcgaagag cgccgagcgg ggcgggcagt tctctctcgg caaaccgacg
     1741 gagggcgtca gatgaggtcg gagccgggga ggatcaaatc gaggaaatgg acgttgccgc
     1801 cttcatcgcc gacgatcacc accctgttca ggacagctac cgaagtgact gagtggtcga
     1861 aggtgaacac agccaagcag gcgcctgtcc ccagatccca cagacgtagc gtcccatcgc
     1921 ctgaccctga gagcgcctgg tcctccccca ccgccgccaa tgcgttgacc gaaccggtat
     1981 ggccaaagaa gatgcgcaga caggcgcccg tttcaagctc ccacagacgc acggtgaggt
     2041 cgagcgagcc cgataatccc cgccctctac ccactgccgt caccgcagtg actggaagga
     2101 gatgcctctc gaaagtacgc aggcaggcgc ccgtatcgac gtcccacaga cgcacagtac
     2161 cgtcccttga gcctgacaac gcccgccgct cccccacagc cgccaacgcg cagacctcgg
     2221 tgttcccttc gaacgtgcgc aggcaagcac ccgtcttagc attccacagg cacagcatgc
     2281 cgtcagaacc cagtattgca ttcccctctc ccaccattgc cagaacattt gcgccagctt
     2341 gcccctcaaa ctgacgcagg caagcgcccg ttctaacatt ccatagacgc agcgagccgt
     2401 cacgcgagcc cgatagcgcc agccgtcccc ccacagccgt caacgcgtaa attccggtgt
     2461 gcccattgaa ggtgcgtagg caggcgcccg tcttaacatc ccacagacac aggggaccgg
     2521 tggtcgcgcc tgatagcgcc tgccccttct ctaagatcgt cactgaacaa accgaaccag
     2581 tatgctcgct acgcaggaaa gccgcaggca ggaggctcgc gcgtcgaggc aggaggccgg
     2641 agccgccaga aagaagtagt cgctcttttc cggcggcacg ggcctcctgc atgaggcgct
     2701 cccatcccca gatatcccct tccggaagcc ggcccacgag ctgacccgcc aactgctcgg
     2761 gatagaggat gagagtcaat accgagcggt aaatcacact tcgtactatg cggagatcag
     2821 ggtcgtggag aagataatca aagccgccga ttaacgctat tacattcgtt gcctggagcc
     2881 tcgccgcaag aaagcggaaa ttgagaagga gctcgcgtag gatgtcgctg tggccgccat
     2941 gtaccaagtg gtagggcagc cagcgccaga gatagctctc cgaaagtggc agatcagccc
     3001 agcggccact tgctgaccgg ctccgcgcga ggaagtcttg gtgcagcgcg gcgatggcgt
     3061 cgccctgtcg gccaatgagg tacttgcgaa atacgtcgtg gagacgaagg gtaccggcgt
     3121 cgaggtcgag gctcaggagc agggagagcc gatgaagccg gcgggcgagt tgcttcgcct
     3181 cgaacgccga gcacgaccac aggcgctcga cgatctgcag cggcacgtct tcgtcctccg
     3241 ggaagatcgc caaggcctcg aaccttcgct gctcgtcctc tgagagccgc cggagactca
     3301 cttcgagggt tcggctcacc gccttctccc gctcggagct gtacttctgg tcgaacgctt
     3361 cgagaccggc agcgtcgaga ccctcgccga tctcgtctag ggccttctcg accccgagtc
     3421 catcctctac taggtctcgg agcgttcggt tgacgagctt gagcaggatc ggccactcgc
     3481 cgagggtacg ggccaaatcc tcgaggcgcg accggaggcc ctccgcgact ggcagctccg
     3541 ccgtgaggag cttcaccgcc tcgtccagcc gcatggcgtc gacatcgacg caggcacagc
     3601 cgtcgggcag ggtttcgcgg agccgggtcg tgacgaggag ccccgtcccg gctccacgga
     3661 agggctggac gtcggccggg ctccaggcgt cgtccaccac gaccaggacc cgccggccgg
     3721 cgaggatctc gcggaggcga gcggcggcgg cctcgacggt gtcgaagtga ggcaagtcgc
     3781 ctggagtcca gaagcggatc aggtccagga cccgggacag gcggtcggcc tcgctcgcct
     3841 ggtcgccgat cgtggtccac aggatggcgt cgggataggc ggcgcggacg cggtcgtcat
     3901 ggcagagagc ctgggccagg gtcgtcttgc cgaaccctcc ggcccctcgg agcgccgagg
     3961 tgatgcccac cgccgaggcg ccgctcccgc agagaatgtc gcggatctga tccaactcct
     4021 cgcgctcgac gaagccctcg ggacggatgg ccatgcagcg gtagccggtg ggcgcaggat
     4081 cgatcagcgc ggggaagtac ttccggagga ggtccggatg gtcttccagg tggtggcaga
     4141 tctcgtccca cgagacgacc gtcaccggaa agccggcgtc cgtaatcttc cgggccgcct
     4201 cctggcaatg gacatcgctc cgggcggtcg tggcgatgat gaaccgggag agcgccggct
     4261 cgaacgtccg cgccttctct acctcttcct cgatcacctt cggcgtcaga ttctcttcaa
     4321 gacgcttgca ctgcacgccg gcccactccc ggccgccttc gggccggccg tagacgtcca
     4381 ccccggcctg cggctggccc tgccggccgt gcttctgggc gtccgggtcg ttccagagcc
     4441 ggcgccagag gtcgagacag agatcttcga agtcctgcca gttgcgcagg ggcgggaggg
     4501 cgagggggcg gggcattgag gaagagttta aagcaccgga ggggccggcc cgccctcccc
     4561 aagagcaccc ccccacctcg atcctccccc gctccctccc gcgccaccgc ccaggggggg
     4621 aggaagcccc ataggcgcta acttagcgtc ttcatggcac gaggtcctat aatctcgtgc
     4681 catggaacct ttggcgatcc ttaaaacgca gtgggaatcc ctcctggcaa tgttgccccg
     4741 ccaga
//
LOCUS       DASPJI010000526         6027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_496028, whole genome shotgun sequence.
ACCESSION   DASPJI010000526 DASPJI010000000
VERSION     DASPJI010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496028"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VEL74_14255"
     CDS             complement(<1..874)
                     /locus_tag="VEL74_14255"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HYG63740.1"
                     /translation="MPSADSPLASLDEMMARLDRVLAASPADTTELAWLEIRRGRESN
                     AKYRRDTFQYRERTILVRVRQSGRIGYHRTEAADLSDLENAMRVALAQARLADPVPAE
                     QWPVPASPGPLPPAEEPGLFDPEVARLTQGGVKERVQKLADRDTADSQVRIGWAEGRV
                     AVANSAGLRRSAAATAVSIEVSCGRGPGAGRAAAASRHLAALDPRGVVEQARRRQASA
                     GREPGEAPDGLVPLILSQEAVARILDLLNRYALSAASFREGTSLLRGHLGNPVFSRTV
                     SLRDDPTDPRGLPFP"
     gene            1001..2698
                     /locus_tag="VEL74_14260"
     CDS             1001..2698
                     /locus_tag="VEL74_14260"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HYG63741.1"
                     /translation="MTRLTPVFVGFLCGIALLGAALPSYAEDSADLILRQGVFYPVQP
                     AARVEGSLAIREGRIVYLGPDAGAEPLRGRQTRVIELNGRMVTPGLIDAHSHLAGLGA
                     ALEQVDLGGAATYEEVVRRVREAAAKVPAGSWVFGRGWDQNLWPGQQFPTHEALSAAV
                     PGHPVWLTRVDGHAALVNARAMTTLGIGPQTQDPAGGRFLRDAQGRPTGVLIDNAMNL
                     PGGGEVPEASADDLKRQIRLAARHCLERGLTTVTDMGIGDAVYRAYSDLRRAGELPLR
                     ASLFLTDDAALLDSWFRRGAEVDPKARLQVRGVKLYADGALGSRGAALVEPYSDDPGN
                     LGLLTTPGAHLLDVARRAGAAGFQVAIHAIGDRGGVVALDALEQAMGGPNPGVRFRLE
                     HAQVLRLQDIERMARLGIIASMQPTHATSDMPWADERVGEARLAGAYAWRKVLAANGR
                     LALGSDFPVEQADPRLGLYSAVTRQDLQGEPAGGWLPGERLTREEALRGFTLDAAWSL
                     FLEKEVGSLEVGKRADLVVFARDWMTAPEAEIPRIEVDMTLVDGQVVHDRAAGGRAR"
     gene            2695..3411
                     /locus_tag="VEL74_14265"
     CDS             2695..3411
                     /locus_tag="VEL74_14265"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HYG63742.1"
                     /translation="MNGRALRPLAAAGLVLLALGAGSRAAGDDFMPQRKAMIARLRQH
                     GITKPEVLAAMQHVPRHLFVPDAVQAEAYSDKPLEVGQGRTIYQPYVVALMTSLLELE
                     RGDKVLEVGTGSGYHAAVLSRIGGEVYSMEIVAPVARQAAKKLGILGYKNVHIRVGDG
                     YQGWPDQGPFDAILLSAAPPTIPKPLLKQLKVGGKMVAPVGGFFQDLQVITKTADGIE
                     TRAIIPVRLSPMTGKVRDGQ"
     gene            3401..4144
                     /locus_tag="VEL74_14270"
     CDS             3401..4144
                     /locus_tag="VEL74_14270"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014706158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HYG63743.1"
                     /translation="MGSSTRKHSAAVLLGLWLLGLAPLASIASAGADEHKADRRRMVE
                     QVRERGVVDPRVLAAMERVPRHLFVPRAQQGEAYDDKPLAIGWGQTISQPSIVGLMTS
                     LLDVKPGDKVLEIGTGSGYQSAVLSRMGVEVYSIEILEPLGEQARKTLARLGYGNVHL
                     RIGDGYKGWPAEAPFDAILLTAAPPEIPKPLLDQLKRGGKMVVPVGTFFQDLQVLTKH
                     RDGSVEKKSVAAVRFVPMTGQAQERKGGH"
     gene            4226..4912
                     /locus_tag="VEL74_14275"
     CDS             4226..4912
                     /locus_tag="VEL74_14275"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HYG63744.1"
                     /translation="MELERESILGLDNIRLGLPVAGVGSRTLAGLVDYLCLGVVVAAW
                     LVICAVIGWLDGDWGIALVVVGLFLLEWCYFSILEVVTGGRTLGKRAVGLRVVTAEGA
                     APGAGALVTRNLVRSLDLLFGVPLMALDPMSRRLGDRLAGTVVVHDRRRGPEPLLGRT
                     PPGWGAREVAVVEAFLAREGELEDAGRSREMALRLLARVERDAPGFLDGVDQSDPVRA
                     LREALQVERV"
     gene            4912..5889
                     /locus_tag="VEL74_14280"
     CDS             4912..5889
                     /locus_tag="VEL74_14280"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HYG63745.1"
                     /translation="MEYSRFVRLRRPVWEAFEARLRGLDGAGRSGRAGLSHQGLEELA
                     FQYRQVLHDHALAAARYPGTAAAGRLEALALEGTRRLTHGGREGGGGLRRFFSHTFPA
                     AVRRHLGALGVATGLFFLAAAFGLVLAMLQPGVGVSFLGPEAIEGLAEGSLWTDSLVT
                     SVPPAFSSSAIATNNIGVALTAWSGGVLAGLVPLWVVLLNGFMLGSIVSVTMHYSMAG
                     ALLEFVAAHGPLEITLILVSAGAGLAIGRAVVAAGDRPRGLAIQEAARDALYVLLGCV
                     PWFGVLALVEVFISPSPELPPAAKAGLGLALEVLFLTLALRRPSPSRSR"
BASE COUNT          736 a         2164 c         2247 g          880 t
ORIGIN      
        1 acgggaaggg gaggccgcgg ggatcggtgg gatcgtcgcg caggctcacc gtccggctga
       61 acaccgggtt gcccaggtgc ccgcggagca gcgacgtccc ctcgcggaac gaggccgcgg
      121 acagcgcgta gcggttgagc agatcgagga tgcgcgccac cgcctcctgg gagaggatca
      181 gggggacgag gccgtcgggc gcctcgcccg gctcccgtcc ggcggaggcc tgccggcgcc
      241 gcgcctgctc cacgacgccg cgcgggtcga gcgccgccaa gtgccgcgaa gccgcggcgg
      301 cccgcccggc gccggggccg cggccgcagc tcacctcgat cgacaccgcc gtggccgccg
      361 ccgagcgccg cagcccggcg ctgttggcca ccgccacccg cccctcggcc cagccgatgc
      421 gcacctggct gtcggcggtg tcgcgatcgg ccagcttctg caccctctcc ttgacgccgc
      481 cctgggtcag ccgcgccacc tcggggtcga agaggccggg ctcctcggcg ggcggcaggg
      541 ggcccggaga agcggggacc ggccactgct ccgccggcac cgggtccgcg agccgtgcct
      601 gggcgagcgc cacccgcatc gcgttctcca ggtccgacag gtccgccgcc tcggtgcggt
      661 ggtagccgat acggccggac tgccgcaccc gcaccaggat ggtccgctcc cggtactgga
      721 aggtgtcgcg ccggtacttg gcgttcgact cccggccgcg gcggatctcg agccaggcga
      781 gctccgtggt gtccgccggc gaggcggcga gcacccggtc caggcgcgcc atcatctcgt
      841 ccagggacgc aagaggggag tcggcggacg gcatgggcgg gaagtctaac gcaagtgact
      901 gagcttcaac gcggtggccc gcggcctgcc ccgggtatgg cttgtccttt gcaacccatg
      961 gctgtcagaa tggtcagccg aactgggaga tatggttgaa atgactaggc tgacgcctgt
     1021 tttcgtgggt tttctctgcg gcatcgcgct gttgggcgcg gccctgccct cctatgcaga
     1081 ggacagcgcc gacctcattc tgaggcaggg cgtcttctat ccggtccagc ccgctgcccg
     1141 ggtggagggg agcctcgcca ttcgcgaggg gcgcatcgtc tatctggggc cggacgccgg
     1201 cgccgagccg ttgcgcgggc ggcagacgcg ggtcatcgag ctgaacggcc gcatggtgac
     1261 tccgggtctc atcgacgccc actcgcacct cgccggtctg ggggccgccc tcgagcaggt
     1321 ggacctgggc ggcgccgcga cctatgaaga ggtcgtgcgc cgggtgcgcg aggcggcggc
     1381 caaggtgccc gccggctcct gggtgttcgg ccgcggctgg gaccagaacc tctggcccgg
     1441 ccagcagttc cccacccacg aggcgctgtc ggcggcggtg cccggccatc ccgtctggct
     1501 cacccgcgtg gacggccacg ccgcgctggt caacgcccgg gcgatgacga cgctggggat
     1561 cggcccgcag acgcaggacc ctgccggcgg ccgcttcctg cgcgacgccc agggccggcc
     1621 caccggcgtg ctgatcgaca acgccatgaa cctgcccgga ggcggcgagg tgccggaggc
     1681 gtcggccgac gatctgaagc gccagatccg gctcgcggcg cgccactgcc tggagcgcgg
     1741 cctgaccacg gtgacggaca tggggatcgg cgacgcggtg taccgtgcct actccgacct
     1801 gcgccgggcc ggcgagctgc cgctgcgcgc ctcgctcttc ctcaccgacg acgcggcgct
     1861 cctcgatagc tggttccggc gcggcgccga ggtcgacccc aaggcgcggc tgcaggtgcg
     1921 cggcgtcaag ctctacgccg acggcgccct cggcagccgc ggcgccgccc tggtggagcc
     1981 ctacagcgac gacccgggga acctcggcct gctgaccacg ccgggcgcgc acctgctgga
     2041 cgtggcgcgc cgggcgggag ccgccggctt ccaggtggcg atccacgcca tcggcgaccg
     2101 cggcggggtg gtggccctgg acgcgctgga gcaggcgatg ggcggcccca accccggggt
     2161 gcgcttccgc ctggagcatg cccaggtgct gcgcctgcag gacatcgagc gcatggcgcg
     2221 gctggggatc atcgcctcca tgcagccgac ccacgccacc tcggacatgc cgtgggccga
     2281 cgagcgggtg ggcgaggcgc gcttggccgg ggcctatgcc tggcgcaagg tgctcgccgc
     2341 caacggccgg ctggcgctgg gcagcgactt ccccgtcgag caggccgatc cgcggctcgg
     2401 cctctattcc gcggtcaccc gccaggatct ccagggggag ccggccggcg gctggctccc
     2461 cggcgagcgg ctgacccgcg aggaggcgct gcgcggcttc accctggacg ccgcctggtc
     2521 gctcttcctg gagaaggagg tgggctcgct cgaggtcggc aagcgcgccg acctggtggt
     2581 cttcgcgcgc gactggatga ccgcccccga ggcggagatc ccgcgcatcg aggtcgacat
     2641 gacgctggtg gacggccagg tggtccatga ccgggccgcg ggaggtcggg cacgttgaac
     2701 ggccgtgccc tccggccgct cgccgccgcg ggcctcgtcc tgctcgccct gggggccggt
     2761 tcccgggcgg ccggcgacga cttcatgccg cagcgcaagg cgatgatcgc gcggctgcgg
     2821 cagcacggca tcaccaagcc ggaggtgctg gcggcgatgc agcacgtgcc gcgtcacctg
     2881 ttcgtgccgg acgcggtcca ggccgaggcc tacagcgaca agccgctgga ggtggggcag
     2941 gggcgcacca tctaccagcc ctacgtggtg gcgctgatga cctctcttct cgagctcgag
     3001 cgcggcgaca aggtcctgga ggtgggcacc ggctcgggat accacgccgc cgtgctgtcg
     3061 cgcatcggcg gcgaggtcta ctcgatggag atcgtcgctc cggtggcccg gcaggcggcg
     3121 aagaagctgg ggatcctggg gtacaagaac gttcacatcc gggtagggga cggctatcag
     3181 gggtggcccg accaggggcc cttcgacgcc atcctcctct cggcggcgcc gccgaccatt
     3241 cccaagccgc tgctcaagca gctcaaggtg ggcgggaaga tggtggcgcc ggtgggcggt
     3301 ttcttccagg acctgcaggt gatcacgaag acggccgacg gcatcgagac gcgggcgatc
     3361 atcccggtcc ggctgtcgcc gatgacgggt aaggtgagag atgggcagta gcacgagaaa
     3421 acattccgcc gcggtcctcc tgggcctgtg gctcctgggg ctggcgccgc tggcctcgat
     3481 agcgtccgcc ggcgcggacg agcacaaagc ggaccgccgc cgcatggtgg agcaggtgcg
     3541 cgagcgcggc gtcgtggacc cgcgggtgct cgccgccatg gagcgggtgc cgcgccacct
     3601 cttcgtcccc agggcgcagc agggcgaagc ctacgacgac aagccgctcg ccatcggctg
     3661 ggggcagacc atctcgcagc cgtcgatcgt cggcctgatg acctcgctcc tcgatgtcaa
     3721 gcccggcgac aaggtgctcg agatcggcac cggctcgggg taccagtcgg cggtgctgtc
     3781 gcgcatgggg gtcgaggtct attcgatcga gatcctcgag ccgctgggcg agcaggcgcg
     3841 caagaccctc gcccgcctgg ggtacggcaa cgtccacctg cgcatcggtg acggctacaa
     3901 gggttggccc gccgaggcgc cgttcgacgc catcctgctg accgccgcgc cgcccgagat
     3961 ccccaagccg ctgctcgacc agctcaagcg cggcggcaag atggtggtgc ccgtcggcac
     4021 cttcttccag gacctccagg tcctcaccaa gcaccgcgac ggcagcgtcg agaagaagag
     4081 cgtcgccgcg gtccgcttcg tccccatgac cggccaggcc caggagcgca agggcggcca
     4141 ctgaaacgtc cggccgcgcc gcgacactct tcctccggga ggttctggta ggcttcgccc
     4201 tacccacccg gaggagagct gcgaagtgga gctggagcgg gagagcatcc taggactgga
     4261 caacatccgg ctcggcctgc ccgtagccgg cgtcggcagc cgcacgctcg ccgggctggt
     4321 cgactacctc tgcctgggag tggtggtggc ggcgtggctg gtgatctgcg ccgtgatcgg
     4381 ctggctggac ggcgactggg gcattgccct ggtggtcgtg ggcctgttcc tcctcgagtg
     4441 gtgctacttc tccatcctgg aggttgtgac cggcgggcgg accctgggca agcgcgccgt
     4501 cggcctgcgg gtggtgaccg ccgagggggc ggcccccggc gccggtgccc tcgtgacccg
     4561 taacctggtg cgcagcctgg acctgctgtt cggcgtgccg ctgatggcgc tcgacccgat
     4621 gtcgcgccgc ctcggcgacc ggctcgccgg cacggtggtc gtccacgacc ggcggcgcgg
     4681 gccggagccg ctcctggggc ggacgccgcc gggctggggc gcgcgggagg tggcggtggt
     4741 cgaggccttc ctcgcccgtg agggcgagct cgaggacgcc ggccggtccc gcgagatggc
     4801 gttgcggctc ctcgcccgcg tcgagcgcga cgcccccggg ttcctcgacg gcgtggacca
     4861 gtccgatccg gtccgggcgc tgcgcgaggc gctccaggtg gagcgggtct gatggagtac
     4921 tcccgcttcg tccgcctgcg gcgcccggtc tgggaggcct tcgaggccag gctccgcggg
     4981 ctggacggcg ccggtcgctc cggccgtgcc ggcctcagcc accagggcct cgaggagctg
     5041 gcctttcaat accgccaggt gctccacgat catgccctgg cggcggcccg ctacccgggc
     5101 acggcggccg ccggccggct ggaggccctc gccctcgaag ggacgcgccg gctgacccac
     5161 ggcgggcgcg aggggggagg cgggctgcgc cgctttttct cccacacctt ccccgccgcc
     5221 gtccgtcgcc atctgggggc cctgggggtg gcgaccggac tcttcttcct ggcggccgcc
     5281 ttcggcctgg tgctcgccat gctgcagccc ggcgtcggcg tctccttcct ggggcccgag
     5341 gcgatcgagg gcctggcgga ggggagcctg tggaccgact ccctggtgac ctcggtgccg
     5401 ccggccttct cgtcgtcggc catcgccacc aacaacatcg gcgtggcgct gaccgcctgg
     5461 tcgggcggcg tcctggcggg cctcgtgccg ctctgggtcg tcctgctcaa cggcttcatg
     5521 ctcgggtcta tcgtcagcgt cacgatgcac tattcgatgg ccggcgccct cctggagttc
     5581 gtcgccgccc atgggccgct ggagatcacg ctgatcctgg tgtcggcggg ggccgggctc
     5641 gccatcggcc gcgcggtggt ggcggcgggg gaccggccgc ggggcctcgc catccaggag
     5701 gcggcgcggg acgcgttgta cgtgctgctc gggtgcgtgc cctggttcgg cgtcctcgcc
     5761 ctggtcgagg tgttcatctc tccgtctccg gagctgccgc cggcggccaa ggccgggctc
     5821 ggcctcgcct tggaggtcct cttcctcacc ttggcgctgc ggcgcccctc tccctcgagg
     5881 tcccgatgat cgctcccacc cgtcgtcccg gtcccatgcc gttcacggtg ctgatggacc
     5941 aggccatgcg cgagacgcgg cgccactggc gcccgatcct gccctcggtg gccctgccct
     6001 tcgccctcct cgcctcggcc gtgacgg
//
LOCUS       DASPJI010000527         2636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_666456, whole genome shotgun sequence.
ACCESSION   DASPJI010000527 DASPJI010000000
VERSION     DASPJI010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666456"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VEL74_14285"
     CDS             complement(<1..973)
                     /locus_tag="VEL74_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG63746.1"
                     /translation="MREPREPGVAERLSALTPEQRALLELLRKKKARTTGQRVHLPPP
                     VPRVSGPTGEGDWPLAFDQEWLLEVVEAAPESNAYNVDATTRLLGRLHYPALEAAFNE
                     IVRRHAAWRTNFPRVDGRRVQRVIPHRMQTLLVVDLTAAPVERRERLAQLALLQVTQE
                     PFDLDHGPLVRAVFARLTPEEHACLVTVHHLVTDWIAFHIFWSELAALYDAALKGLPS
                     PLPELPLQYVDYAVWLRQWLQGEALEELAGYWRNKLSNFPLVLELPTDRPRRPDRRMK
                     GGRYIIDSGPEPAERLRVLAQREGATMFMATFAVVYALLHRFTGQEKI"
     gene            complement(966..>2636)
                     /locus_tag="VEL74_14290"
     CDS             complement(966..>2636)
                     /locus_tag="VEL74_14290"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KR domain-containing protein"
                     /protein_id="HYG63747.1"
                     /translation="RRVVHLWAVDPAAEPLPFARSQEPGLLSLLFLAQALSEAGVTDP
                     LHLTLVSSHLHEVAGGEPVEPAKATLLAALKVLPQEYPHWTCRGVDVVPPAAGSAGED
                     RVVERLLAEASAPGPDVAVAWRGRDRWARAYDRVRLDGAPAGAAVRLREQGVYLVTDA
                     LDSLGLEVAEWLVRTWKAKVALLVPPDFPERRDWEPLLAGSQTGDEQALRSIRRLSEL
                     EGAGGEIWTLPASLTDRAQLQGALARVRERHGALHGVFHTAVLTAGGLVQLKTAEALA
                     ADLAPQVEGAALLTTLLASEPVDFLVLCGSTLTASGGFGQLEIAAGGLVLDALADALT
                     AAAGEGPLTVAVHWDPYQWDGWLALGAGGGQLLSAEAMQENLALYGISWERSGEALSR
                     LLAAGLPRVVVSSTDLEALIRQADQITAATLMEQMQEARRSAPKHARQLATPYAEPRT
                     ELETEIAAAWEDLFGIAPIGIHDSFLELGGHSLLAIQVTTELRKRYEVDLPVTAVFEA
                     PTVAELAASIERAREAAEMELAELLAAVEGLSPEEALRRMQEEMQTHA"
BASE COUNT          362 a          975 c          963 g          336 t
ORIGIN      
        1 cgatcttctc ctggccggtg aaccggtgga gcagggcgta gacgaccgcg aaggtcgcca
       61 tgaacatggt cgctccctcg cgctgcgcca ggacgcgcag gcgttccgcc ggctccgggc
      121 cgctgtcgat gatgtagcgc ccccccttca tccgccggtc cggccggcgg ggccggtcgg
      181 tgggcagctc aaggaccagg gggaagttgg agagcttgtt gcgccagtag ccggccagct
      241 cctccagcgc ctccccctgc agccactgcc gcagccagac cgcgtaatcc acgtactgca
      301 acggcagctc ggggaggggc gacggcaggc ccttgagcgc ggcgtcgtag agcgccgcca
      361 gctcgctcca gaagatgtgg aaggcaatcc agtccgtcac caggtgatgc acggtgacca
      421 gacaggcgtg ctcctccggc gtcaggcgcg cgaacaccgc gcgcaccagc ggcccgtgat
      481 cgagatcgaa cggctcctgc gtcacctgga gcagggcgag ctgcgccagc cgctcccgcc
      541 gctcgaccgg agcggccgtg agatcgacca ccagcagcgt ctgcatacgg tgggggatca
      601 cgcgctggac gcggcggccg tccacccgcg ggaagttggt gcgccaggcg gcatggcgcc
      661 gcacgatctc gttgaaggcg gcctccagcg ccgggtaatg gagccgcccc agcagacggg
      721 tcgtggcgtc gacgttgtag gcgttgctct cgggagccgc ttccaccacc tcgaggagcc
      781 actcctggtc gaacgccagc ggccagtccc cttccccggt cggccccgac acacggggga
      841 ccggcggcgg caggtgcacg cgctgccccg tcgtcctggc cttcttcttc cgcagcagct
      901 cgagcaacgc gcgctgctcg ggcgtcaggg ccgacagcct ctccgccact ccaggctcac
      961 ggggctcacg catgggtctg catctcctct tgcatccgcc gcaacgcctc ctcgggcgac
     1021 agcccctcga ccgcggcgag cagctccgcc agctccatct ccgcggcttc gcgcgcccgt
     1081 tcgatggagg cggccagctc ggcgacggtg ggggcctcga acaccgcggt caccggcagg
     1141 tcgacctcgt agcgcttgcg cagctcggtg gtgacctgga tggccagcag cgagtgcccc
     1201 ccgagctcga ggaagctgtc gtggatgccg atgggggcga tgccgaacag gtcctcccag
     1261 gcggcggcga tctcggtctc cagctcggtg cgcggctccg cgtacggggt cgcgagctgg
     1321 cgggcatgct tgggggcgga gcgccgcgct tcctgcatct gctccatcag ggtggcggcg
     1381 gtgatctggt ctgcctggcg gatcagcgcc tccaggtcgg tcgacgagac caccacccgc
     1441 ggcagcccgg cggccagcag gcgcgacagg gcctcgccgc tccgctccca cgagatgccg
     1501 tacagcgcca ggttctcctg catggcctcg gcggagagga gctgcccgcc gccggccccc
     1561 agggcgagcc agccgtccca ctggtagggg tcccagtgca ccgccacggt caaggggccc
     1621 tcgccggccg ccgccgtgag cgcgtccgcg agcgcgtcga ggacgaggcc gccggccgcg
     1681 atctcgagct ggccgaagcc gccgctggcg gtcaacgtcg acccgcagag gaccaggaag
     1741 tccaccggct cgctggcgag cagcgtggtc agcagggccg cgccctccac ctggggcgcg
     1801 aggtcggcgg ccagggcctc ggcggtcttg agctggacga ggccgccggc ggtcagcacc
     1861 gcggtgtgga agacgccgtg cagcgcgccg tggcgttccc ggacccgcgc cagggccccc
     1921 tggagctgcg cgcggtcggt gaggctggca ggcagggtcc agatctcccc gcccgcgccc
     1981 tccagctcgg agaggcgccg gatgctgcgc agggcctgct cgtcgcccgt ctggctcccg
     2041 gccagcagcg gctcccaatc gcgccgctcg gggaagtccg gcggcaccag cagggctacc
     2101 ttcgctttcc aggtccgcac cagccactcc gccacctcga gcccgagcga gtcgagggcg
     2161 tcggtgacga ggtagacccc ctgctcgcgc aggcgtactg ccgcgccggc cggagccccg
     2221 tcgagccgga cccggtcgta ggcccgcgcc cagcggtccc ggccgcgcca ggcgaccgcg
     2281 acgtccgggc cgggcgccga ggcctcggcc aacaggcgct cgaccacgcg atcctcgccg
     2341 gcggagccgg cggcgggcgg caccacgtcg acgccgcggc aggtccagtg cgggtactcc
     2401 tgcggcagga ccttgagagc ggcgaggagg gtggccttgg ccggctccac cggctccccg
     2461 ccggccacct cgtggagatg gctcgacacc aaagtcaggt gcagcggatc ggtgacgccg
     2521 gcctcggaca gcgcctgggc caggaagagg aggctgagga gaccgggctc ctgcgagcgc
     2581 gcgaagggca gcggctcggc ggcggggtcc acggcccaga gatggaccac ccggcg
//
LOCUS       DASPJI010000528         7598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_33536, whole genome shotgun sequence.
ACCESSION   DASPJI010000528 DASPJI010000000
VERSION     DASPJI010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33536"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="VEL74_14295"
     CDS             complement(<1..1596)
                     /locus_tag="VEL74_14295"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HYG63748.1"
                     /translation="MSDPERWRRIDGLFAEALELPPDERQGFLAAACAGDDELRSEVE
                     SLLAADAEGEEGAAFLEQPAGEWLDLDEAGGPERRLGPYRLLRRLGAGGMGSVYLARR
                     DDGLYEREVAVKILRSGLEGTGALHRFLAERQILARLEHPGIARLYDGGRTTGGRPFL
                     VMELVDGLPLDEYCDRHRLGVEERLRLFVRICAAVQHAHQNLLVHRDLKPANILVTAE
                     GEPKLLDFGIAKRLGPESADGADGSGPVTRTGLRVMTPSYASPEQVRGEAITTASDVY
                     SLGVLLYELLAGRSPYRIAGGLPHEVERAICDDEPERPSQALLRISGDRPPEEIAAAR
                     GTRPQALRRRLRGDLDTIVLEALRKEPRRRYGSAARLAEDIERHLRNLPVAARPDSLL
                     YRTRKLLRRRRAAAIAVTLGALLALGFVLSLVEQGRRLARERDKARYALSFLVEAFKN
                     ADPYHTRGRRLTAEEVLEQGAARVARDLHGRPEVQAAVLDAIGEVYLGQGRADAAAPL
                     LERALAMRRQDPGAAPADLANTLE"
     gene            complement(1593..2147)
                     /locus_tag="VEL74_14300"
     CDS             complement(1593..2147)
                     /locus_tag="VEL74_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG63749.1"
                     /translation="MTQWLQRWSEGDKEAAARALPLVYDELRRIAARQLRQERGDHTL
                     EATAIVHEAYLRLDEQDGLHWPSRTHFFAFAAHLMRRILVDHARHLNRAKRGAGTQRV
                     TLSEAADLALPRSPDLEAVDDALRALEEVDPRKAAVVELRFFAGLTIEETAGQLGVSA
                     ETVGREWRRAKAWLYRELSRERSA"
     gene            3329..3478
                     /locus_tag="VEL74_14305"
     CDS             3329..3478
                     /locus_tag="VEL74_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63750.1"
                     /translation="MDGGLQPAADRDGEVFVLFGRRPDLVGWPVTLAGSPPEELLERV
                     RHLAR"
     gene            5149..5385
                     /locus_tag="VEL74_14310"
     CDS             5149..5385
                     /locus_tag="VEL74_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015177794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63751.1"
                     /translation="MRSNDFPPGLDEQGVRDLIEYYESQTDEEAAAEHEAALASPTGT
                     LMEVPTELVPLVRDLIARHLKKRQSREKSWAPGS"
     gene            complement(5944..7527)
                     /locus_tag="VEL74_14315"
     CDS             complement(5944..7527)
                     /locus_tag="VEL74_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63752.1"
                     /translation="MRRQDGRVVLAAMVLAVTGVGLFGCGPGGRTSQKKQQLLAQPPA
                     NPCTGTGQNVALTPTVPLQFSSFGAQLGADCMAWQTFIALNWAADPNNPGHPNPNAPA
                     SAFGTPGDTSPKVWESYLEASAVFNPVSQTGALWKAARPAVKQLSRTSKFGMVDLSLS
                     DIGQAGDGKWLTSQAGGLTYYEVRINQDEFEFITTNVFSGSDLTTYAGQAACAGQAGQ
                     AGKGGFNLPGGGGASGATLDVDCMGNPKIYGQNQGAIEIKASWIALPADHSLDYRYKT
                     AVAQITDPFGHVSQVTVGLVGLHILHKTPGAPQFVWATFEQIDNSPDEGGAGGFTAPT
                     LPANPNQKPRSAYAFFNPNCTAASDPTYNCVHNQLPGTPCDASGQPAGCDPYTAPMQV
                     TRIVPVDTNANSVTGYVWSLLPSQSVFNYYRLANVMWPNSPMAVAPQSTIPLPTGDIT
                     PPDNAGRVANTTLETYQQTQNSCMDCHKFASIAQTGQRTVMKIAGKNVSKVRPVAKSG
                     ANATYASDYSFIFASETVK"
BASE COUNT         1140 a         2483 c         2692 g         1283 t
ORIGIN      
        1 ctcgagggta ttcgccaggt cggccggagc ggcgccgggg tcctgacggc gcatcgccag
       61 cgcgcgctcc agcagcggtg ccgccgcgtc ggccctcccc tgtccgaggt agacctcgcc
      121 gatggcgtcg aggacggcgg cctggacctc gggccggccg tggaggtcgc gcgccacccg
      181 ggccgccccc tgctcgagca cctcctcggc ggtcaggcgc cggccgcggg tgtggtaggg
      241 atcggcgttc ttgaaggcct cgaccaggaa cgacagcgcg tagcgcgcct tgtcgcgctc
      301 gcgggcgagg cgccgcccct gctccacgag gctcagcacg aagccgaggg cgagcagcgc
      361 gccgagggtg acggcgatgg ccgccgcgcg ccggcggcgc agcagcttgc gggtgcggta
      421 gagcagcgag tccggccggg cggcgaccgg caggttgcgc aggtggcgct cgatgtcctc
      481 ggcgagccgg gcggcggagc cgtaccgccg ccgcggctcc ttgcgcagcg cctcgaggac
      541 gatggtgtcc aggtcccccc ggagccgccg gcgcagcgcc tgcggccggg tgccgcgggc
      601 cgcggcgatc tcctccggcg gccggtcgcc ggatatgcgg agcagggcct ggctgggccg
      661 ctcgggctcg tcgtcgcaga tggcgcgctc cacctcgtgc ggcaggccgc cggcgatccg
      721 gtaggggctg cggccggcga gcagctcgta gagcaggacc ccgagcgagt agacgtcgct
      781 cgccgtggtg atcgcctcgc cgcgcacctg ctcggggctg gcatagctgg gggtcatcac
      841 ccgcagcccg gtgcgcgtca ccggccccga gccgtcggcg ccgtcggcgc tctccggccc
      901 gagccgcttg gcgatgccga agtcgaggag cttcggctcc ccctcggcgg tcaccaggat
      961 gttggcgggc ttgaggtcgc ggtggaccag caggttctgg tgggcgtgct ggaccgcggc
     1021 gcagatgcgc acgaagaggc gcagccgctc ctcgacgccg agccggtgcc ggtcgcagta
     1081 ctcgtcgagg gggagcccgt cgacgagctc catgaccagg aacggccggc cgccggtggt
     1141 gcggccgccg tcgtagagcc gggcgatgcc ggggtgctcg aggcgggcga ggatctgccg
     1201 ttcggccagg aagcggtgca gggcgccggt gccctcgagg ccggagcgca ggatcttgac
     1261 cgccacctcg cgctcgtaga ggccgtcgtc ccggcgggcc aggtagacgc tgcccatgcc
     1321 accggcgccg aggcggcgca gcagccggta ggggccgagg cggcgctccg ggcccccggc
     1381 ctcgtcgagg tcgagccact cgccggccgg ctgctcgagg aaggcggcgc cctcctcgcc
     1441 ctccgcgtca gccgcgagga ggctctcgac ctcgctccgc agctcgtcgt cgccggcgca
     1501 ggccgcggcg aggaagccct gccgctcgtc cggcggcagc tcgagggctt cggcgaagag
     1561 tccgtcgatc cgtcgccagc gctccgggtc gctcaagcgc tccgctcccg ggacagctcg
     1621 cggtacagcc aggccttggc gcggcgccac tcgcggccga cggtctcggc cgagacgccg
     1681 agctggccgg cggtctcctc gatggtgagg ccggcgaaga agcgcagctc cacgaccgcg
     1741 gccttgcgcg ggtcgacctc ctcgagcgcg cgcagcgcgt cgtccaccgc ctcgaggtcc
     1801 gggctcctgg ggagcgccag gtcggcggct tcggagagcg tgacccgttg cgtccccgcg
     1861 ccgcgcttgg cccggttgag gtggcgggcg tggtcgacca ggatgcgccg catcaggtgg
     1921 gcagcgaagg cgaagaagtg ggtgcggctc ggccagtgga ggccgtcctg ctcgtcgagg
     1981 cgcagatagg cctcgtggac gatcgccgtc gcctccagcg tgtggtcgcc gcgctcctgc
     2041 cggagctgcc gcgcggcgat cctgcgcaat tcgtcataga ccaggggcag ggcgcgcgcc
     2101 gcggcctcct tgtccccctc gctccaccgc tgaagccact gcgtcactct cgcctgatcc
     2161 ggactgtcac ggatcatgac cccaaggata tcccggaatg gggacagacc cggggcggcg
     2221 cgctcccagt ccgcagggct ttatatttca gagattcctt tctttcgccc agagagaggc
     2281 gtgactctcc cggacgccgc ggacgagatc cggacgcagc ggatcgcgtc cggacgcccc
     2341 ggatcgcttc ccgagcgctc cggatgcgat ccagacgccg cggacggcat ccgaacgctt
     2401 cggatcgctt cccgagcgcc tgggaagcga tccaaacgct ccggacgcca tccgagcgct
     2461 tcggattctt cccgagccgc ccggaaggaa tccggagcgt ccgggagggc tcggagcggt
     2521 cccgaaggcc ttggagacgt tcggatggag tcggggagtc ccggtcgtag gccgagcggc
     2581 ccggctcggc tttgagtctc cgggatctca gccgagccgc tgggaggggc tcctgtctgg
     2641 acgagagcgg tctctggctg aatggggacg gctccggtct acggtgggag cccgtactgc
     2701 cagcggaaag gtccatcacc ccggcgcgat ggctcgtccc tcgctggtcc agtggcacct
     2761 gtgcaaggca gttgcagcct attgccggcc tgttgccgga acagaacagg cgccaggcac
     2821 ctcttctcaa ggccttgctg gatggcaagg cgccccaaga gttgctccgg ggcgagtggg
     2881 accccgaaga ccctggtccc aagcgggtac gcgagctggc ccgctcgctg accgcctctt
     2941 ctgccacgtg atcgtctatc gccacgcgga tccgcgcttt cccttccttt gggaagacgc
     3001 cgagcaaccc tccgcccgct ggcaccggtc tggagagggg cccgtgcact actttgccga
     3061 tactccgaat ggggcctggg cggagctcct ccgtcacgag gagattcgcg agcccggtga
     3121 cctggccggg atccagaggg ccctctgggc cgtcgagctt ccggtcgtgc cggaggattc
     3181 ccccgactta ccgggggagg tcctctctgg aggtctcgag gtctacccgc aatgccagca
     3241 agaggctgcc cgtctgcacc tcctcgggat ccacggattc cgcgtcccct cggcagctct
     3301 cctgcctggc ggcgccgggg gatggatcgt ggacggaggt cttcagccgg cggcagaccg
     3361 ggacggcgag gtcttcgtgc tgttcgggcg ccggcccgac ctcgtcggct ggccggtgac
     3421 gctcgccggc tcccctcccg aagagctgct ggagcgggtc cgtcacctgg cccgctgaaa
     3481 gagcgcttgg cacgtccttc ggtcggcgag tgccgctgca catctactgg tggtacatag
     3541 ccctccctgc cacaatccca ccccatgacc ctgggaagca tcgacctcaa agtcgtcctc
     3601 gggtggttgg gcatcgtatt ggaagcgtta caggagctgc gggctctccc ggcggattcc
     3661 ctgaggtgga gagtctccat cctggaccgg gtggtgtcct tactcagtgt gggaggggcg
     3721 gagcggggga ggcacggcta gccgcgcacc ctagccgcgc ccgcttagca gggatacttt
     3781 gtccagcatt gaatcggacc agtcgggcag ccttgtgtgc acacgagcgt acacccgttc
     3841 gtgcactcca ctacagttgc tccgccctcc acttgggaga acaggccccg caccgtctcc
     3901 ttcgagagca ccagcttctt cgtcttcttc ttcatcgcca ctccttagta ggtacgcgcc
     3961 acctggcgca ggggcattgt accgggtagc tactccctcc accagcttgc gctaagggat
     4021 tgttttggcc ctcccttctc cgagctggag agctcaatat cgagtaccag gttcatgtag
     4081 accgccacgg gcccgccctc gtatcgggcg ggctcgaagc gccactctct aagcgtttcc
     4141 accagcgcgg cttcgagggc cggacccttc ggcccccgca ggacctgagc cctgagcacc
     4201 ataccgtctt cggagatcac ggtgcgcacg atcagagatt ccgggggcga ttctcctggg
     4261 cctgggctcc agcgcaccgg gaccctgtgc gagaccttcg gaaccgtcga cgcttgccag
     4321 tagatgcggc ggtcagagcc ctctccgagg gagtccggcc gaacggtcag ccagaggcct
     4381 tcgggagagg gtgcagcgcc atgcgacgga ccggcaccgc cgggcgaagc cggcttcggc
     4441 gctgatcgat cgccgcagct cgcgcttcca aggaggagcg agagacagag gagggcgagc
     4501 gacctccact tcggaacgca tgggagtcgg agcgaacgtc tggagggctc atgttcggct
     4561 ctttcgttgc gtgagcacat gggatcaggg tacattgttc gtgcaatgga ggccagagag
     4621 gtagctttcg tcagtttcgg cagtatctgg atgaggagat tggccaagtc atcgccggcc
     4681 atggggtgct gcacgtgcgc cggaaggagg caggcgactt cgtcgtccta ggcgccggta
     4741 actggcgcgc cgttgaggag tctttgtacc tccagcaggt tccaggactc tcggcgagcc
     4801 tccacgaggc agctcgcgag cctctcgaag agagcacccg attggaggat ctcgattggt
     4861 gaagcgggtt gctgcgtcga agagccaagt acctggcact tcctgcgggt tccttcccga
     4921 gcacaaccgt tcgctcaagg gagtcctgtc gacgtacgtc cagcgctcgt tggctccgga
     4981 ggactggtcc tcggtccgta aacaggcttg ggaggatgcg tccgtcgagg attggaagcg
     5041 ggagggagaa tcgggtgcgt catgaaatcg gccgaagccg cccggagaac agagaacagg
     5101 tgcctggcac cacctccggg cagcctaccg tcaccgcagg agacaggcat gagatccaac
     5161 gacttccctc ccggtttgga tgagcagggt gtccgagacc tcatcgagta ctacgagagt
     5221 cagacggatg aggaggcggc tgccgagcat gaggcggctc tcgcgagtcc gacggggacg
     5281 ctcatggagg tcccaaccga gttggtgccg ctcgtcaggg acttgatcgc ccgtcatctg
     5341 aagaagcgcc agtcgcgaga gaagagctgg gccccgggct cctgaggcca gatccctcgc
     5401 gagatccggc gtgcttcgcc gtcgccggat cgcctcatcc ttccttgccc tacatccaac
     5461 cccctctccc caagggtgag gcctcaagaa caagtgcctg gcacctcctg agggatggcc
     5521 cgccaagtgg gctagagggc gcgcagcgcg ttagcaggag caggtgttcc ggcaaaggga
     5581 gccggggcat atcgccccgt tttggctctc gtagattccg cagtagctcg ccgggccgga
     5641 ggggcaaccg caggtgaaac cttcggtctc gttgctgcaa ttgtcggtga acgcaccgcc
     5701 agcgacgtgc ccgcgcccca agctgagatc ccgtagcgcc tccttcgcca gcacccagct
     5761 tcttcgaatg cgagggcaat cacgcatcag caccgccgtt cggaccgcac agccccccac
     5821 ccggaccgcg ccgacaaagg tcagggccaa cctgccggtt cgagggcaag gccgtccctt
     5881 acgcgtctct caccacgctg ggatcgcggc cccgggagtc gtggaggacc ggagcggatg
     5941 cgtctatttt actgtctcgc tagcgaagat gaacgagtag tccgaggcgt aggtcgcgtt
     6001 ggcgccggac ttggccaccg ggcggacctt gctaacgttc ttgccagcaa tcttcataac
     6061 tgtgcgctgg ccggtctggg cgatggaggc gaacttatgg cagtccatgc acgaattctg
     6121 ggtctgctgg taggtctcca gtgtggtgtt ggccaccctg ccggcgttgt cgggcggagt
     6181 gatgtcgccg gtcggaagcg ggatcgtgct ctggggcgcc acggccatgg ggctgttggg
     6241 ccacatcaca ttggccaggc gatagtagtt gaacaccgat tggctcggca gcaggctcca
     6301 gacatagccg gtgacgctgt tggcattggt gtcgaccggt acaatgcggg ttacctgcat
     6361 cggggcggtg taggggtcgc agcccgccgg ctggccgctg gcgtcgcagg gcgttccggg
     6421 gagctggttg tgtacgcagt tgtaggtcgg atcgctcgcc gcggtgcagt tggggttgaa
     6481 gaaggcgtag gccgagcgcg gcttttggtt gggattggcc ggaagggtcg gcgccgtgaa
     6541 gccgccggcc ccgccttcat ccgggctgtt gtcgatctgc tcgaaggtgg cccagacgaa
     6601 ctgcggggcg cctggcgtct tgtgaaggat gtgcagtccg accaagccga cggtgacctg
     6661 actgacgtgg ccgaacggat cggtaatctg ggcgactgcg gtcttataac gatagtcgag
     6721 ggaatggtcg gcgggcagcg cgatccacga ggccttgatc tcgatcgcgc cctggttctg
     6781 accatagatt ttcggattgc ccatgcagtc tacgtcgagc gttgcgccag aggctccgcc
     6841 gcctccgggc aagttgaagc cacccttgcc ggcctggccg gcctgcccgg cgcaggcggc
     6901 ctgtccggca taggtagtca ggtcggagcc tgagaacacg ttggtggtga tgaattcgaa
     6961 ttcgtcctgg ttgatgcgga cctcgtaata ggtgaggccg cccgcctggc tggtcagcca
     7021 cttgccgtcg ccggcctgac cgatgtcgct gagcgacagg tcgaccatcc cgaacttgga
     7081 ggtgcggctg agctgtttga ccgctggacg cgcggccttc cacagcgccc cggtctggct
     7141 gaccgggttg aagacggccg aggcctccag gtagctttcc cagaccttgg gcgaggtatc
     7201 gccgggtgtg ccgaaggcgg aggccggagc gttggggttg ggatggccgg ggttgttggg
     7261 atcggccgcc cagttcaacg cgatgaaggt ctgccaggcc atgcagtcgg cgccgagctg
     7321 ggcgccgaac gaagagaact gaaggggaac cgtcggggtc agggcgacgt tctggccggt
     7381 gccggtgcag ggattggccg gcggctgggc caggagctgt tgttttttct gtgaggttct
     7441 tccccccggc ccgcagccga agagaccgac cccggtgaca gccagaacca tggcggccag
     7501 gaccaccctt ccgtcttgcc ttctcacggt ttctccctcc tatacgatgt agatcgccgc
     7561 cggcaagatc gaagctccgg cgacggagtc gatgatct
//
LOCUS       DASPJI010000529         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_779968, whole genome shotgun sequence.
ACCESSION   DASPJI010000529 DASPJI010000000
VERSION     DASPJI010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779968"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VEL74_14320"
     CDS             complement(<1..801)
                     /locus_tag="VEL74_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63753.1"
                     /translation="MPLTPPRNRRLRPSAPSKPAERKPIYAPPRVSVPVPGAGSGSGL
                     FAKLWAGGLASGFLLFFMSISLGILLFGVAGTVGPYRDSLRQGNDPGTFYGAMGMGTL
                     FLLVAGRMLQIVLTSVEVEKRWKKKGKKSEPWTWDHPWRPTGMGPDYVSGAGGYLFGR
                     IAFLGFIGFFNIAWGSQSWLLRGAILLFDAFGLVILLDSLRALVQWLRFGRAEVAWKT
                     FPAFLGERLEATVRFPRTLHPTGPARVTLRCVQDEWTTRRTSRGEERRE"
     gene            complement(868..1842)
                     /locus_tag="VEL74_14325"
     CDS             complement(868..1842)
                     /locus_tag="VEL74_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HYG63754.1"
                     /translation="MMSIVLSASLLMVLMAAWGFGGRLPWRRGQEGTVPADRLAVHVQ
                     TLAERLAPRDAYHAENLDRVAAYIREELERAKGTVSEQPFEVGGTTYRNVIAHFGPET
                     EERIVVGAHYDAAGPLPGADDNASGVAGLIELGRLLGASPPKLRVELVAFALEEPPHF
                     GSSSMGSAVHAGSLKRRGVPVRAMICLEMIGYFSDEPESQRFPLPGLRWIYPSRGNFI
                     AVVGRPGEGGLAGRVKKAMQRATPLPVHSATLPSSVPGVGLSDHRNYWDAGYNAVMIT
                     DTAFFRNDNYHTEEDTPETLDYRRMAQVVQGVHAAVLGEAARRGARRK"
BASE COUNT          345 a          760 c          702 g          315 t
ORIGIN      
        1 ctcgcggcgc tcctctcccc ggctcgtcct ccgggtcgtc cactcgtcct ggacacagcg
       61 cagcgtcacc cgcgccggtc cggtgggatg cagcgtgcgc gggaagcgga cggtggcctc
      121 cagccgctcg ccgaggaacg cgggaaaggt cttccaggcc acttcggcgc ggccgaagcg
      181 gagccactgg acgagagccc gcagcgagtc cagcaggatc accaggccga aggcatcgaa
      241 cagcaggatg gcgcccctga gcaaccagct ctgtgatccc caggcgatgt tgaagaaacc
      301 gatgaagccc aggaaggcga tcctgccgaa gagatagccg cccgctccgc tcacgtagtc
      361 gggtcccatc cccgtgggac gccaggggtg gtcccaggtc cacggctccg acttcttgcc
      421 cttcttcttc cagcgcttct ccacctccac cgaggtgagc acgatctgga gcatgcgccc
      481 ggccaccagc aggaacaggg tccccatccc catggccccg tagaaggtcc cgggatcatt
      541 gccctgccgc aagctgtcgc ggtagggccc gacggtcccg gccactccga agaggaggat
      601 cccgaggctg atgctcatga agaacaggag gaagccgctg gccaatccgc ccgcccacag
      661 cttcgcgaac aggccgctgc cggagccggc accggggacc ggcaccgaga cccggggcgg
      721 cgcgtagatg ggcttcctct cggcaggctt gctgggtgcg gacggccgca gtcgccggtt
      781 ccgtggtggt gtcaggggca tgagggtccc gatccgaacg gcgaagactc tatccgatcg
      841 ggggcgcctc gagccgggcc gttcctctca ctttctgcgc gcgccacgcc gggcggcttc
      901 acccaggacc gccgcgtgga cgccctggac gacctgggcc atccgccggt agtcgagggt
      961 ctccggcgtg tcctcctcgg tgtgatagtt gtcgttgcgg aagaaggccg tgtcggtgat
     1021 catgaccgcg ttgtagcccg cgtcccagta gttccggtgg tccgacaggc cgactccggg
     1081 gacggagctg gggagggtcg ccgagtggac gggcaagggg gtggcccgct gcatcgcctt
     1141 cttgacgcgc cccgccagcc cgccctcccc cggccgtccc acgacggcga tgaagttccc
     1201 ccgcgaagga tagatccatc tcaacccggg cagcgggaac ctctggctct ccggctcgtc
     1261 gctgaagtag ccgatcattt cgaggcagat catggcccgc accggcacgc cgcgccgctt
     1321 gagagatccc gcgtggacgg cgctccccat cgaggagctg ccgaagtgcg gcggctcctc
     1381 cagcgcgaag gcgaccagct cgacccgcag cttcgggggt gaagctccca gcaggcgccc
     1441 gagctcgatc aggcccgcca cgccgctggc gttgtcgtcc gcgccgggaa gtgggcccgc
     1501 cgcgtcgtag tgggctccga ccacgatccg ctcctccgtc tccggaccga aatgggcgat
     1561 cacattgcga taggtcgtcc cccccacctc gaacggctgc tcggagacgg tccccttcgc
     1621 ccgctcgagc tcttcccgga tgtaggcggc tacccggtcg aggttctccg cgtgatacgc
     1681 gtcgcgcggc gccaacctct cggccagcgt ctggacatgg acggcgagcc ggtccgccgg
     1741 caccgtcccc tcctgccccc gcctccacgg cagacggcca ccgaaacccc aggccgccat
     1801 cagcaccatc aggagactcg cggacaacac gatcgacatc atcgttccag cacgcctcct
     1861 catcctggag cggggttgca ggacgggtgc cagacggacc gaaccccgga gccgcgggat
     1921 ctcaacgcat cctcccgctg ctccgtatac atagatgtaa gggcccgaga tcgagctatc
     1981 tgacgggggg atatccggag ggaaaccctc gtaccggtac gggctcgtac gaatgcggcg
     2041 accgtgcgca cgggcgcgcc gtcgcggggt ggtcccgcgg cggcctccaa agacttgctc
     2101 ctcgcaagag aggaaaggga tc
//
LOCUS       DASPJI010000530         9554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_666598, whole genome shotgun sequence.
ACCESSION   DASPJI010000530 DASPJI010000000
VERSION     DASPJI010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666598"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VEL74_14330"
     CDS             complement(<1..918)
                     /locus_tag="VEL74_14330"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYG63755.1"
                     /translation="MNERSGFFRRHATRLTALLLLLVLYGFGRLPTLPQEERDAMASR
                     FAFERTALPDPTDSPEALRRSIRPVHPSYAGIAAWISSVGAGVGLHDLDGDGLANDAC
                     RVDTRVDRVVVSPVPGTGDRFPLFTLDPDPLLPHDRATMAPMGCLPGDWNEDGRADLL
                     VYYWGRPPVAFLRRAGAEGMAAAAFRPVAVAPGSERWNTNALTSADVDGDGHLDLVVG
                     NYFPDGSAILDANDRGTAHMHGSMSRSENGGKNRLLVWKSGSAGPEPSVAFADVPEAF
                     DHETTYSWTLSVGAADLDGDLRPEIYFGND"
     gene            complement(915..1877)
                     /locus_tag="VEL74_14335"
     CDS             complement(915..1877)
                     /locus_tag="VEL74_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015141367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1702 family protein"
                     /protein_id="HYG63756.1"
                     /translation="MGSLSKRLFGISPDETRFATRRFRCDVPDVRERLEEVGRCFVRG
                     YHAALEEDRPIPLAERLEAITPEFRGFAYEGAGMALAILDALTPWRRGRVEAFLRGPA
                     EPHIYLVLVGAGWALARLPLSAERMLARFETVKRWLVLDGYGFHQGYFHWPRAIAGQE
                     RPRRLSGYALRTFDQGIGRSLWFVEGAGIDRIADRLAAFPTERRGDIWSGVGLACTYA
                     GGVGAADIEDLKRAAGEHAPQLAQGAAFAAKARARAGNLTPHTELACRVICGTGAEAV
                     AAVTDAARVDLPPDQPANGLPAVPAFEVWRQRIQQSFVPGGNAV"
     gene            complement(2009..2761)
                     /locus_tag="VEL74_14340"
     CDS             complement(2009..2761)
                     /locus_tag="VEL74_14340"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HYG63757.1"
                     /translation="MRDYFVFLLLLLIKSLARLFYRLDVQWIGDPPPDAWHKHKIVAI
                     LNHTSLYEALFAGVCPNHFHWRLAHHGVLPLAQKTAERAVVGRFYSLMAKRVIPITRQ
                     RDESWKEVLRQIDPDAMVMILPEGRMKRANGLDSTGKPMTVRGGIADIVETIGDGRML
                     LAYSGGLHHIQVPGERWPRLFKTIRLRLELIDIGPYRDELMAQAGGPRGFKRLVVDDL
                     ERRRDLYCPIEPGTDSPLKAPQPQGEPSPPLS"
     gene            2862..3761
                     /locus_tag="VEL74_14345"
     CDS             2862..3761
                     /locus_tag="VEL74_14345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYG63758.1"
                     /translation="MPERPTSRPTLSAIITTFNEEQHIAECIESLLWCDEILVVDSFS
                     TDRTPEIAQRYPKVRFSQRTYFGSASQKNWAMDQVTNDWILIFDADERCTPELQKEIE
                     ALLARGPKEEAYTIRRRVWFLGKVIRFSGWQHDQVVRLVRTGSARYPNRRVHADMITR
                     GPAPLLRNPMEHYMTDSLDEYARRIEKYSFWGAAQNWKEGKRSGPLEVFGRAVWRFLR
                     TYIFQLGVLDGMHGLVFCMLQGYGTYLKWALLWGWHVNAARGIQPNLPIFDDSETTWR
                     GLDEEAAGAAQGSEAAEPAGKER"
     gene            3809..4054
                     /locus_tag="VEL74_14350"
     CDS             3809..4054
                     /locus_tag="VEL74_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HYG63759.1"
                     /translation="MRAVSLHYAQTHLSHLVDQARAGEEIVIQEGETPLVRLTPVPVQ
                     RERVFGIDEGKVVIPDDFDDPMPELEDMFYNSPDDPA"
     gene            4041..4427
                     /locus_tag="VEL74_14355"
     CDS             4041..4427
                     /locus_tag="VEL74_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG63760.1"
                     /translation="MILLDTHVWLWLNIAVEEIPSEILKTLKQREQKIYLSAASAWEI
                     AIKHASGKLPLPLPPEAYIPSRMEMNGILPLPILHGHAVKAASLPPHHRDPFDRMLIA
                     QAQVENLRLVTADPWMSDYDVEILWR"
     gene            4452..5624
                     /locus_tag="VEL74_14360"
     CDS             4452..5624
                     /locus_tag="VEL74_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009546958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HYG63761.1"
                     /translation="MDASLPQLLLDHLRRHQDEMVDFLRGLVLAESPTLVPESQEEVF
                     ARLAEGLAAAGYRTRRLRGRTSGGQLWAAPRERRRRSGRMQLLLGHSDTVWPLGTLAS
                     MPVELRDGRLTGPGSFDMKGGLTQGVFALRALAELGLEPPVPPAFFINSDEEMESPDS
                     VRQIRRLAHHAERVFVLEPAFGPEGRLKTGRKGTGHFQVTVHGRAAHAGLDPQKGASA
                     ILGLSHAIQQLHALSDFERGTTVNVGVISGGSRRNVVAPEARGVVDVRVLRQEDAAAV
                     ERAIRSLDPVVPGTKIEVAGGMVKPPLEPTPRNRALWQAAQRCAGELGFELGEATVGG
                     ASDGNTTSQYTPTLDGMGAVGDGAHAAHEYVEVARMPERAALLAMMLMQPATSAGG"
     gene            5714..6409
                     /locus_tag="VEL74_14365"
     CDS             5714..6409
                     /locus_tag="VEL74_14365"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HYG63762.1"
                     /translation="MFILVVFAVSFAGLPARAWAGSSNPVLTKAEIRQAEQWLKELGY
                     WTGPVDGVWDATSRHALTAFQKVERARPTGRLTRAEWNALSMARPPRPKEKGLGGPLH
                     LEVDIARQVLFLVDADGKVGNVVPISSGSGKRFYEKGYPETLAITPCGRLEVYQKVSG
                     WRTSPLGEMHNPLYIVGGIAIHGSQDVPAYPASHGCIRIPMFASNSLHKMVPKGTPVL
                     VYGCKEEGTATAP"
     gene            6440..6667
                     /locus_tag="VEL74_14370"
     CDS             6440..6667
                     /locus_tag="VEL74_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015145192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HYG63763.1"
                     /translation="MRAEEFDRRFDEGEDVTGVLNPSAARRPGQEERRVNVDFPAWMV
                     ESLDREAKRLGVTRQSLIKVWIAEKLDRKAS"
     gene            complement(6874..7389)
                     /locus_tag="VEL74_14375"
     CDS             complement(6874..7389)
                     /locus_tag="VEL74_14375"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYG63764.1"
                     /translation="MAECPRPLAEIPAGTKVFLDSTIFLYHFTGVSAQCRGLLERCAA
                     REVRALTSTLVLAEVTHRLMTIEATERGLVTPGNVVKKLRERPDLIRQLSEYQAQVEK
                     IPWMGIEVVALERRTLATAGPLRSRYALLTNDSLLAATALERSAMAFASADRDFESVE
                     GFQLYGPTDLE"
     gene            complement(7382..7621)
                     /locus_tag="VEL74_14380"
     CDS             complement(7382..7621)
                     /locus_tag="VEL74_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63765.1"
                     /translation="MRRKFSTLLDEHLYDRARLEARRQGKQIADVVDEALTEYLDERS
                     ALRGPADVVAGSWGALRLPKERVRQLLEEEGLLDG"
     gene            complement(7832..>9554)
                     /locus_tag="VEL74_14385"
     CDS             complement(7832..>9554)
                     /locus_tag="VEL74_14385"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HYG63766.1"
                     /translation="LPPRERKLRFSVAPEPKYEDARTRELGEKLAALQRQLKEQTIAG
                     GDVTDVREQILGLKRSMRDGERLQAGDWLAEGRFQLLEPAGRGGFATVWKAWDEERQA
                     LVAVKVLHGLHAEDRTRRERFFRGALKMAELHHPGIVRVIEVELEENGKHFFVMDYVG
                     GGDLRRAVLAGELTQEKVLPVILAVGEALAYAHGKGITHRDVKPANILLDGNRPKLTD
                     FDLVRAFDTTGGTQTQDMLGTMLYAAPEMFDDAKNASAAVDVFSLAMTAVFAFHGQDL
                     PRGVLRRPEFLLEKLPCSPALREALGRGISWEPEGRPGSVTELCDELRLASAPIIEPA
                     AGEERLNEVDGTVLLYVPGGEYTLGAENVHKAATPIHRVVLRPFWIAKHPVTNEQYGR
                     FLKANAGAPVPQYWRDKSFNKPEQPVVGVSWDEAQVYCRWAGLRLPSEAQWEAAARGT
                     DGRRYPWGDVEPTPEHANFGNRIGCPTPVGSFPKGAGPFGALDQAGNVWEWCEDAWDE
                     AAYRERTGKRDPVSTTGDPTVRHVRGGAWGDYVGPLAAAYRSWHRASFRNGVIGFRCV
                     SPAVPEH"
BASE COUNT         1520 a         3239 c         3251 g         1544 t
ORIGIN      
        1 gtcgttgccg aagtagatct ccggccgcag gtcgccgtcc aggtcggcgg cgcccaccga
       61 cagcgtccag gagtaggtcg tctcgtggtc gaaggcctcc gggacgtcgg cgaaggcgac
      121 cgacggctcg gggccggcgc tgccgctctt ccacaccagc agccggttct tgccgccgtt
      181 ctccgagcgc gacatcgagc cgtgcatgtg ggccgtgccg cggtcgttgg cgtccaggat
      241 ggcggagccg tcggggaagt agttgccgac cacgaggtcg aggtggccgt cgccgtcgac
      301 gtcggcgctg gtcagggcgt tggtgttcca gcgctccgag ccgggggcga cggccaccgg
      361 ccggaaggcg gcggcagcca tcccctccgc gccggcgcgg cgcaggaagg cgaccggcgg
      421 acggccccag taatagacca gcaggtcggc gcggccgtcc tcgttccagt cgcccggcag
      481 gcaccccatg ggcgccatgg tggcgcggtc gtgggggagg aggggatcgg gatccagggt
      541 gaagaggggg aagcggtcgc ccgttccggg caccggcgag accaccacgc ggtcgacccg
      601 tgtgtcgacg cggcaggcgt cgttggcgag gccgtcgccg tccaggtcgt ggagcccgac
      661 gcccgcgccg acgctggaga tccaggccgc gatgccggcg tagctcggat gcaccggccg
      721 gatggagcgc cgcagcgcct ccggcgagtc ggtggggtcg ggcagcgcgg tccgctcgaa
      781 ggcgaagcgc gaggccatgg cgtcccgctc ctcctgtggc agcgtcggca gacggccgaa
      841 gccgtacagc acgagcagta ggaggagggc tgtcagccgg gtcgcgtggc gccggaagaa
      901 gccggaacgt tcgttcatac tgcattacct cctgggacaa agctttgctg gatccgttga
      961 cgccagacct cgaaggcggg caccgcgggc aggccgttcg ccggctggtc cggcggcagg
     1021 tcgacccgcg ccgcgtcggt gaccgcggcg accgcttcgg cgccggtgcc gcagatcacg
     1081 cggcaggcga gctcggtgtg gggagtcagg ttaccggcgc gggcccgggc cttggcggca
     1141 aaggcggcgc cctgggcgag ctgcggggca tgctcgcccg cggcccgctt gaggtcctcg
     1201 atgtcggcgg cgccgacacc gccggcgtag gtgcaggcga gcccgacgcc gctccagatg
     1261 tcgccccggc gctcggtggg gaaggcggcc agccggtccg cgatgcggtc gatgccggcc
     1321 ccctcgacga accagaggct gcggccgatc ccctggtcga aggtgcgcaa ggcatagccg
     1381 gagagccgcc gtggccgctc ctggccggcg atcgcccgcg gccagtggaa atagccctgg
     1441 tggaagccgt agccgtcgag caccagccat ctcttgacgg tctcgaagcg cgccagcatg
     1501 cgttcagcgg agagggggag ccgggcgagc gcccagccgg ctccgaccag gacgaggtag
     1561 atgtgaggct ccgccggccc gcggaggaac gcctccaccc ggccgcggcg ccacggggtg
     1621 agggcgtcga ggatggccag ggccattccc gctccctcat aggcgaagcc gcggaattca
     1681 ggggtgatcg cttccagtct ctccgccagg ggaatcggcc ggtcctcctc gagtgccgcg
     1741 tggtagccgc ggacgaaaca gcgtcctacc tcttcgagcc gttccctcac atccgggaca
     1801 tcacaacgaa aacgccgagt ggcgaatctg gtctcatccg gcgagatgcc gaagaggcgc
     1861 ttcgatagac tacccatcag ccctccctgc ccgggtcagg agtcgctggc ttcggaggtg
     1921 atccgtccag ccgaggctcg aggttttttg cctctacttt cctgggtgct ttaaagcggc
     1981 ctggccgagc cacaggcggg agattcgcct aggagagagg aggagagggc tcgccctggg
     2041 gctgcggggc cttgaggggg ctgtccgtcc ccggctcgat ggggcaatag aggtcgcgcc
     2101 ggcgctccag gtcgtcgacc acgaggcgct tgaacccccg ggggccgccg gcctgcgcca
     2161 tcagctcgtc gcggtagggg ccgatgtcga tcagctcgag acggaggcgg atggtcttga
     2221 acagccgggg ccagcgctcg ccgggcacct ggatgtggtg cagaccgccg ctgtaggcga
     2281 ggagcatgcg gccgtcgccg atggtctcca cgatatcggc gatacctccg cggacggtca
     2341 tcggcttgcc ggtggagtcc aggccgttgg cgcgcttcat ccggccctcg ggcaggatca
     2401 tcaccatggc gtcggggtcg atctggcgca gcacctcctt ccagctctcg tcccgctgcc
     2461 gggtgatggg gatcacccgc ttcgccatca ggctgtagaa gcggcccacc accgcccgct
     2521 cggcggtctt ttgggccagg gggagcacgc cgtggtgggc gaggcgccag tggaagtggt
     2581 tcgggcagac gccggcgaac agggcctcgt agaggctggt gtggttgagg atcgccacga
     2641 tcttgtgttt gtgccaggca tcgggcggcg gatcgccgat ccactggacg tccagccgat
     2701 agaagagccg cgccaggctc ttgatcagca ggaggagaag gaagacgaag tagtcgcgca
     2761 cgggtggagg gaattctagc cgggatttca ctccgctgct cgccgcagag cggcacgacg
     2821 tctgccaccg atccgcccgg ggctctgata cgctttgccc catgcccgag cgcccgacct
     2881 cccgccccac tctgtcggcg atcatcacca ccttcaacga ggagcagcac atcgccgagt
     2941 gcatcgagtc gctgctctgg tgcgacgaga tcctggtggt ggactccttc tccaccgacc
     3001 gtacgcccga gatcgcccag cgatatccca aggtgcgctt ctcccagcgc acctatttcg
     3061 gctcggcgtc acagaagaac tgggcgatgg accaggtcac caacgactgg atcctgatct
     3121 tcgacgccga cgagcgctgc acgccggagc tccagaagga gatcgaggcg ctgctggcgc
     3181 gcggtccgaa ggaggaggcc tacacgatcc ggcggcgggt ctggttcctc ggcaaggtga
     3241 tccgcttctc cggctggcag cacgaccagg tggtgcgcct ggtgcggacc ggctcggccc
     3301 gctaccccaa ccgccgagtc cacgccgaca tgatcacccg cggtccggcg ccgctgctgc
     3361 gcaacccgat ggagcactac atgaccgact cgctggacga gtacgcccgg cggatcgaga
     3421 agtacagctt ctggggtgcg gcgcagaact ggaaggaggg gaagcgctcc ggccccctcg
     3481 aggtgttcgg ccgcgcggtc tggcgcttcc tccgcaccta catcttccag ctcggcgtgc
     3541 tcgacggcat gcacggcctg gtcttctgca tgctccaggg atacggcacc tatctcaagt
     3601 gggcgctgct ctggggctgg cacgtcaacg ccgcccgcgg catccagccc aacctgccga
     3661 tcttcgacga cagcgagacc acctggcggg gcctggacga agaggctgcc ggcgccgccc
     3721 agggttccga agccgccgag ccggcgggta aggagcggta gagcggccgg gcgttgagtt
     3781 cgcgggagaa ctgacttaag gttggtccat gcgagccgtc agccttcact acgcccagac
     3841 ccatctttcg cacctcgtgg atcaggcgcg ggccggggag gagatcgtca tccaggaggg
     3901 agagaccccg ctggtccggc tgacaccggt cccggtccag cgcgaaaggg tcttcgggat
     3961 cgacgagggc aaggtcgtca ttccggacga cttcgacgac ccgatgcccg agttggagga
     4021 catgttttac aattctccag atgatcctgc ttgataccca cgtctggctc tggctgaaca
     4081 ttgcggtaga agagatcccc tccgagatcc tgaaaaccct gaagcaacga gaacagaaga
     4141 tctatctctc tgcggcgagt gcgtgggaga tcgccatcaa gcatgccagc ggcaagttgc
     4201 ctctccccct tccgccggag gcctatattc cgtcgcggat ggagatgaac ggcatccttc
     4261 cgcttccgat cctccacggc cacgccgtca aggctgcctc gctccctccc caccaccgtg
     4321 accccttcga ccggatgttg atcgcccagg ctcaagtcga gaacctccgg ctggtgacgg
     4381 cggacccgtg gatgtccgat tacgacgtgg agatcctctg gcgttagggt gacgggtaga
     4441 atcctcccgg catggacgcc tctctccccc aactcctcct cgaccacctt cgtcgccacc
     4501 aggacgagat ggtggacttc ctgcgcggct tggtccttgc cgagtccccc acgctggtgc
     4561 ccgaatccca ggaggaggtc ttcgcccgct tggcggaggg gctcgcggcg gcgggctacc
     4621 ggacgcggcg cctgcgcggc cggacgagcg gcggccagct ctgggcggcg ccgcgcgagc
     4681 gtcgccggag gtccggccgg atgcaactcc tcctcggcca ctcggacacg gtctggccgc
     4741 tcggcaccct cgcctccatg cccgtagaac tgcgcgacgg ccggctcacc ggccccggct
     4801 ccttcgacat gaaggggggc ctcacgcagg gggtcttcgc cctgcgcgcc ctggccgagc
     4861 tggggctgga gccgccggtc cctcccgcct tcttcatcaa ctccgacgag gagatggaga
     4921 gcccggactc ggtgcggcag atccggcgcc tcgcccacca tgccgagagg gtgttcgtgc
     4981 tggagcccgc cttcggcccc gaagggcggc tcaagaccgg ccgcaagggg accggccact
     5041 tccaggtcac ggtgcatggc cgggcggcgc acgccggcct cgatccgcag aagggggcga
     5101 gcgccatcct cggcctctcc cacgccatcc agcagctcca cgccctctcc gacttcgagc
     5161 gcggcaccac cgtcaacgtc ggcgtgatct ccggcgggtc gcgccgcaac gtcgtggcgc
     5221 cggaggcgcg gggggttgtc gacgtgcggg tgctgcgcca ggaggacgcc gccgccgtcg
     5281 agcgggcgat ccgctcgctc gatccggtgg taccgggcac gaagattgaa gtagccggcg
     5341 gtatggtcaa gccgccgctc gagcccaccc cccgcaaccg ggctctctgg caggcggccc
     5401 agcgatgcgc cggcgaattg ggattcgagc tgggggaggc gacggtgggt ggcgcttcgg
     5461 acggcaacac caccagccag tacacgccga cgctggacgg tatgggagcg gtgggcgacg
     5521 gtgcccacgc cgctcacgag tatgtcgagg tggcgcggat gcccgaaagg gcggcgctcc
     5581 tcgctatgat gttgatgcag ccggcgacgt ccgccggcgg ctgagcgtcc gggatatccg
     5641 gacacccggg tggccgcggg gggccactgg agggatgggg gtcacgatgt ggttcggtcg
     5701 tagaagcacg ctgttgttca tcctcgttgt cttcgcggtg tccttcgccg gtcttcccgc
     5761 gagagcctgg gccggcagct ccaatcccgt tctgacaaaa gccgagatcc gccaggccga
     5821 gcagtggctc aaggagctgg gctactggac gggtccggtg gacggcgtct gggacgcgac
     5881 ctcgcgccac gccctgaccg ccttccagaa ggtggagcgc gcccggccca cgggccgcct
     5941 cacccgggcc gagtggaacg ccctctccat ggcccggccg ccgcggccga aggagaaggg
     6001 gctgggcggt ccgctgcacc tcgaggtgga catcgcgcgc caggtcctct tcctggtgga
     6061 cgccgacggc aaggtcggca acgtcgtgcc catctcctcg gggagcggca agcggttcta
     6121 cgagaagggc tatcccgaga ccctggccat caccccttgt ggccggctgg aggtctacca
     6181 gaaggtctcc ggttggcgca ccagcccgct cggggagatg cacaaccccc tctacatcgt
     6241 gggcggcatc gccatccacg gcagccagga cgtcccggcc tatcccgcga gccacggctg
     6301 catccgcatc cccatgttcg cctccaacag cctgcacaag atggtcccca aggggacgcc
     6361 cgtgctggtc tacggctgca aggaggaggg gacggccacg gcgccttgag tgggtggacg
     6421 ccagaggagg tcgctttcga tgagggctga ggaattcgac cgccggttcg acgaaggtga
     6481 ggatgtcact ggtgtcctca atccgagtgc tgcgcgccgt cctggtcagg aggaacgccg
     6541 ggtcaacgtc gacttccctg cctggatggt cgagtctctc gatcgcgagg ccaagcggct
     6601 tggcgtcacc cggcagtctc tcatcaaggt gtggatcgcc gagaagctcg accgtaaggc
     6661 gtcgtagccg gccctccgat cccgagtttg tgcgaaacca atcgctccgt gtgcggaatg
     6721 aagcccatct cccgcggaac gatgttcgcg ccgccaggaa tggacctcgg ttcgtgcgga
     6781 atgacggccg tattgtgcgg gatggcgcct gcgcctcctc ttctctccgc ccccgcccgt
     6841 caccctgagc cggactcggc gcggcgcgga accttattcg agatcagtgg ggccgtagag
     6901 ctggaaacct tcgaccgact cgaaatctcg gtccgcggat gcaaaggcca ttgcgctgcg
     6961 ttcgagcgcc gtggcggcga gcagtgagtc attggtcaac agagcgtagc ggctgcgtag
     7021 aggtccggcg gtggcgagcg tccggcgttc cagggccacg acctcgatcc ccatccacgg
     7081 aatcttctca acctgcgcct ggtattcgga gagctgccgg atgaggtccg ggcgctcgcg
     7141 cagcttcttc accacattgc ctggcgtgac taggccgcgc tcggtcgcct cgatggtcat
     7201 caggcggtgg gtgacttcgg ccagtaccag ggtcgaggtc agagcgcgca cctcgcgggc
     7261 ggcgcaacgc tccagcaagc cgcgacattg ggccgagacc ccggtaaagt gatagaggaa
     7321 gatggtggag tcgaggaaca ccttggtccc ggcagggatc tccgccaggg gcctgggaca
     7381 ctcagccatc cagcaatcct tcttcctcca acaactgacg gacccgctcc ttggggagcc
     7441 gcagcgcacc ccaactccca gccaccacgt ccgccggtcc gcggagagcc gagcgttcgt
     7501 cgagatactc ggtcaaagcc tcgtcgacga catcggcgat ctgtttgccc tggcgcctgg
     7561 cttcgagacg cgcgcggtcg tagaggtgct cgtcgagcag cgtgctgaac ttccttcgca
     7621 tagcgtcatg atggtatcat gacggggctc gaccggcgcg acatctaacc tgccgtcatt
     7681 ctcttcctga gggggcgtcg agtctacctg cccgcccgcc tcatccgctc cagagggcct
     7741 gagcttgcgg acagtgtttt cggctggcta cgacgtggag accaagctct gcccctggtc
     7801 ccccaaatgc acaagagcct caaaccaaga atcaatgctc aggaacggca ggtgacacac
     7861 aacgaaaacc gatgacccca ttccggaacg atgccctgtg ccaggagcgg tatgctgcag
     7921 ctagaggccc gacataatcg ccccaggccc cgccacgcac atgacgaaca gtgggatcgc
     7981 cggtagtgga aacgggatcg cgtttcccgg tgcgctcccg gtaggccgcc tcatcccatg
     8041 cgtcttcgca ccactcccag acgttgcctg cctgatccaa ggcaccaaaa ggtcccgcac
     8101 ccttcggaaa cgatcccacc ggggtgggac aaccaatccg gttcccgaag ttcgcgtgct
     8161 ccggcgtcgg ctcgacatcg ccccagggat atcggcggcc gtccgtccct cgagctgcgg
     8221 cctcccattg cgcctcgctc ggcaggcgaa ggcccgccca gcgacagtac acctgcgcct
     8281 cgtcccagct cacgcccacc accggctgct caggcttatt gaagctcttg tccctccaat
     8341 attggggcac gggtgcgccc gcgttcgctt tcaggaaacg gccgtattgc tcgttcgtca
     8401 ccgggtgctt cgcgatccag aacggtctga gaaccacgcg atgaatcggc gttgcggcct
     8461 tgtgcacgtt ctcggctccc agggtgtact ctccgcccgg cacgtagagc agcactgtgc
     8521 catccacctc gttgagccgt tcctctcccg ccgctggctc gatgatcggc gcggaagcca
     8581 gtctcaactc gtcgcagagc tcggtcaccg agcccggccg tccttcaggc tcccaagaga
     8641 tgccgcggcc gagagcctca cgcaacgccg gagagcaggg gagcttctcc agaaggaact
     8701 ccggccggcg caggacgccg cgcggtaggt cctggccatg gaaggcgaag acggcggtca
     8761 tcgccaagga gaagacgtcc acggcggcgc tcgcgttctt ggcgtcgtcg aacatctccg
     8821 gcgccgcgta cagcatagtc ccgagcatgt cctgcgtctg cgtacctccc gttgtgtcga
     8881 aggcccgtac cagatcgaag tcggtcagct ttggccggtt tccatccagc aggatgttcg
     8941 ccggcttgac gtcgcggtgg gtgattccct tcccgtgcgc ataggccagc gcctctccca
     9001 cggccaggat gacgggtagg accttctctt gcgtcagctc tccggcgagc accgcccggc
     9061 gcaggtcccc tcctcccacg tagtccatga cgaagaaatg cttcccgttc tcctccaact
     9121 cgacctcgat gacgcggacg atccccgggt gatggagctc ggccatcttg agggcgccgc
     9181 ggaagaagcg ctcgcggcgc gtgcggtcct cggcgtggag gccgtggagg accttgaccg
     9241 ccaccagcgc ctgtcgctcc tcgtcccacg ccttccagac ggtggcaaag ccaccgcgac
     9301 cggcgggctc gaggagttgg aaacgccctt ccgcgagcca gtcgcccgcc tgcaggcgct
     9361 ccccgtcccg cattgagcgc ttcaggccaa ggatctgctc tcggacgtcg gtgacgtccc
     9421 cgccagcaat ggtctgttcc ttgagttgcc gctgtaacgc ggcgagcttc tcgccgagct
     9481 cgcgagtgcg ggcgtcctcg tacttcggct ccggagccac cgagaaccgt agcttgcgtt
     9541 ctcggggcgg cagg
//
LOCUS       DASPJI010000531         3690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_496179, whole genome shotgun sequence.
ACCESSION   DASPJI010000531 DASPJI010000000
VERSION     DASPJI010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496179"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="VEL74_14390"
     CDS             complement(<1..1123)
                     /locus_tag="VEL74_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63767.1"
                     /translation="MENSSVEHQALAVNGIHASTGEPLLKEVTLGQIAAVACGETRPA
                     AEVEELRRWSAYVREDHLDTRFQIDLRNLAETGWGVIFPENRQDELRDALAPLLALRR
                     SQAGRLQENRYRELVYRAGDSKLRFLGRHRVSPGPADPDLLPYYLLLVGSPEEISFRF
                     QYQLDVQYGVGRLCFARLEEYARYAAGVVAAEQGEIPATRRASFFGVGHAGDAATAMS
                     LKSLVTPLASRLAERHGDWTVETVLESAATKAALAERMGGARTPAFLFTASHGMGFDP
                     SDPRQAAHQGALLCQDWPGPLAWEGPIPPAHYLAEDDIPDTAAPAGLVAFLFACYGAG
                     TPARDSFEMEGDRRPLAAESFVARLPQRLLGHPGGCALAV"
     gene            complement(1162..1560)
                     /locus_tag="VEL74_14395"
     CDS             complement(1162..1560)
                     /locus_tag="VEL74_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63768.1"
                     /translation="MVSKIGLLLTGVALLLIGAEGCGGPRARSDRSFGEIQALARGKT
                     AEQILRLLGDPDSREVVFDNDERWIWWNYTFLDGQDFPPETRGRVVHLEIVFKNPRRS
                     GHTPVSRPRYSAWEVDDPFGITYRAPSRED"
     gene            complement(1660..1830)
                     /locus_tag="VEL74_14400"
     CDS             complement(1660..1830)
                     /locus_tag="VEL74_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63769.1"
                     /translation="MKNRRSPEQPEHHEAPSVMKGHDFEPEEKSSGAKPDEPATAYTG
                     PHAPPPRGGKQS"
     gene            complement(1987..>3690)
                     /locus_tag="VEL74_14405"
     CDS             complement(1987..>3690)
                     /locus_tag="VEL74_14405"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYG63770.1"
                     /translation="PGQARERYEQALRLSPGDERAFFGLARVARAEGELDEAERWMEQ
                     TLDAIEAARDQVWRLDLRSSYLASRQEQYAFYIDLLAERHRREPDRGHDARAFAISER
                     ARARSLLDLLSVASQKPDPEEVRRVGELSRQVNTQHLDVLASSQGIEDGAPGSDLSDL
                     LERLRQAEAEAQGPLTLERATPTLSLTQVQAELIDGETLLLEYFLGEERSFLWAVTSE
                     ETRFSILPGREEIEQAARQAYRRMLESHHQTGEIAARQAASRLSRMLLGPVADLLDRR
                     RLVLVAPGSLQSVPFAALYHPQSGGRVAAQPLIAEHEIVSLPSASVLATLRTRTANRR
                     PPPGLLAVVADPVFGPDDERLQGLRRGGEAGRARPSSLVTPWQRLRYTGREAEAILAL
                     AGSRSVLAASGFAANRDLVRNGRLGSYRVLHFATHGILNDLYPELSALALSAFDASGR
                     PIDGHLRAYEISDLDLRADLVVLSACRTGLGKEVGGEGLVGLTQGFLHAGAPRLLVSL
                     WDVDDRATGELMKRFYTGLFLQNLPPAQALRQAQLSLSREKRWRAPYYWAGFTLQGEW
                     R"
BASE COUNT          593 a         1246 c         1282 g          569 t
ORIGIN      
        1 tcaccgccag agcgcagcct ccggggtggc cgagcagacg ttgcggcagc cgggcgacga
       61 agctctccgc tgccagcggg cgccgatccc cctccatctc gaagctgtcc cgggccgggg
      121 tgccggcgcc gtagcaggcg aagaggaagg cgacgaggcc cgcgggcgcc gcggtgtccg
      181 ggatgtcgtc ttccgccagg tagtgggccg gcgggatcgg tccttcccac gccaggggcc
      241 cgggccagtc ctggcagagc agggccccct ggtgggcagc ctgccgcggg tccgaagggt
      301 cgaatcccat gccatggctg gcggtgaaga ggaacgccgg ggtgcgggcg ccgcccatgc
      361 gctcggcgag cgccgccttg gtcgccgcgg attccaggac ggtctccacc gtccagtcgc
      421 catggcgttc ggcgagccgg gaagcgaggg gcgtcaccag gcttttcagg ctcatggccg
      481 tggccgcgtc gccggcatgg cctaccccga agaacgaggc gcggcgggtt gccggaatct
      541 cgccctgctc cgccgccacc acgcccgcgg cgtagcgggc gtactcctcc agcctggcga
      601 agcagagccg gcctacgccg tattgcacgt cgagttggta ctggaagcgg aacgagatct
      661 cttccgggct gccgacgagg agcaggtagt aaggaaggag gtccgggtcg gccggtcccg
      721 ggctcactcg gtgccggccg aggaaccgca gcttggaatc gccggcacgg tagacgagct
      781 cgcggtaccg gttctcctgg agccggccgg cctgggaccg gcgcagggcg agaagcggag
      841 cgagcgcgtc gcgcagctcg tcctgccggt tttccggaaa gatcactccc catccggtct
      901 ccgccagatt ccgcagatcg atctggaagc gggtatcgag atggtcctcg cggacgtagg
      961 ccgaccaccg gcggagctcc tcgacctcgg cggccggccg ggtctcgcca caggccaccg
     1021 ccgcgatctg tccgagcgtc acttccttca gcaacggctc gccggtcgag gcgtgaatgc
     1081 cgttcaccgc caaagcctgg tgttcgacag aactgttttc catgccaaga tctcctcgag
     1141 gctgccctga tcgggacgtc tctagtcctc tctcgatggc gcccggtatg tgattccgaa
     1201 aggatcatcg acctcccacg cggaatagcg aggcctgctc accggcgtat gcccggacct
     1261 tctggggttt ttgaaaacga tttcgagatg caccacccgg ccccttgtct cgggagggaa
     1321 gtcctgaccg tcgaggaatg tgtagttcca ccagatccat cgctcgtcgt tgtcaaagac
     1381 cacctcacga ctatcgggat ccccgagaag ccggaggatc tgctccgcgg tcttgccccg
     1441 ggcaagagcc tggatctcac cgaaggagcg gtccgagcga gccctgggac cgccacaccc
     1501 ctcggcaccg atcagcagga gcgccacgcc cgtgagcaga agcccgatct tcgaaaccat
     1561 acgcacctcc ctgatggatg agcgctcccg gagccccctc cgctccgcac ggatgcggaa
     1621 ggcaccgggt gccgggagcg cttgggcgta ttgccgtgct cagctctgct tgccgccgcg
     1681 cggcggtggg gcatgtggac cggtgtaggc ggtggccggt tcatcgggct tggctccgct
     1741 cgacttctct tcgggttcga agtcgtgccc cttcatgacg gacggggcct cgtggtgttc
     1801 cggttgctcg gggctgcggc ggttcttcat ggcgtcctcc gtgctccctc tccggtcccc
     1861 ggagggctcc gggggcggga gggcgctgtt ttgggtccgc gccagggaga agccgcgtac
     1921 ggcgatttgg ttagaggaga gaggagaatt tcgcgagcgg gagaaggagc ggggccagcc
     1981 ttcctctcac cgccactcac cctggagtgt gaagccggcc cagtaatagg gggcacgcca
     2041 gcgcttctcc ctcgaaagcg agagttgggc ttgccgcaaa gcctgggcag gaggcaggtt
     2101 ctgtaagaag aggcccgtat aaaaacgttt catcaactcg ccggtggcgc ggtcgtccac
     2161 gtcccagagc gatacgagga ggcgcggcgc cccagcgtgc aggaacccct gggtcaatcc
     2221 caccagcccc tcgcctccga cctctttgcc gagaccggtg cgacaggcgc tcaacaccac
     2281 gagatccgcg cgcagatcga gatcggagat ctcataagca cggaggtggc catcgatggg
     2341 ccggccggag gcgtcgaagg ccgaaagggc gagggccgac agctcgggat agagatcgtt
     2401 gaggatgcca tgggtggcga agtggaggac gcggtaggat cccagccgcc cgttccgcac
     2461 caggtcccga ttggccgcga aacccgaagc ggccagaacg gaccgggatc cggccagggc
     2521 gaggatggcc tcggcctccc gccccgtgta gcgcagacgc tgccagggag tcaccaagct
     2581 ggagggccga gccctacctg cctcgccgcc acggcggagg ccttggagcc gctcgtcgtc
     2641 cggcccgaag accgggtccg ccaccaccgc caggaggccg ggcggaggcc gccggttcgc
     2701 ggtccgggtg cgcagcgtgg ccaggacgga ggcagagggc aggctgacga tctcatgctc
     2761 tgcgatgagg ggctgggcag ccacgcggcc gccggactgc ggatggtaca gggcggcgaa
     2821 aggaacgctc tgcagggagc cgggggcgac gaggaccaga cgccggcggt cgagcagatc
     2881 ggccaccgga cccaacagca tgcggctcag ccgcgatgcc gcctgacggg ccgcgatctc
     2941 ccccgtctgg tgatggctct cgagcatccg ccggtaggcc tggcgcgccg cctgctcgat
     3001 ctcctcccgg ccaggcagga tcgaaaaccg cgtctcctcc gaggtcacgg cccaaagaaa
     3061 gctccgctcc tcgcccagga aatactcgag cagcagcgtc tcaccgtcga tgagctcggc
     3121 ctgaacctgc gtcaatgaca gcgtgggggt ggcccgttcg agtgtaagcg gcccttgggc
     3181 ttccgcctcc gcctgccgca agcgctccag gagatccgac agatccgatc ccggtgcgcc
     3241 gtcctcgatt ccctgcgaag aggcgagcac gtcgagatgt tgcgtgttga cctgccggct
     3301 cagctcgccg acacggcgca cttcctcggg atcgggtttt tgactcgcca cgctcaggag
     3361 atcgagcagg ctcctcgcgc gggcacgttc gctgatggcg aacgccctgg cgtcgtggcc
     3421 tcggtccggc tcgcggcggt gccgctctgc caggaggtcg atgtagaacg cgtactgctc
     3481 ctgccgggag gcgagatagg acgaccgcag atccagccgc catacctgat cgcgggccgc
     3541 ctcgatcgcg tccagggtct gctccatcca gcgctcggcc tcatcgagct cgccctccgc
     3601 cctggccacg cgggccagtc cgaagaaggc acgctcatcc cccggggaca gccgcaacgc
     3661 ctgctcatag cgttcgcgcg cctggccggg
//
LOCUS       DASPJI010000532        19139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_330771, whole genome shotgun sequence.
ACCESSION   DASPJI010000532 DASPJI010000000
VERSION     DASPJI010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 19139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19139)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19139
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330771"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..259)
                     /gene="gvpJ"
                     /locus_tag="VEL74_14410"
                     /pseudo
     CDS             complement(77..259)
                     /gene="gvpJ"
                     /locus_tag="VEL74_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009626980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpJ"
     gene            complement(263..646)
                     /locus_tag="VEL74_14415"
     CDS             complement(263..646)
                     /locus_tag="VEL74_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009712793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HYG63771.1"
                     /translation="MPSSIEPSYRRSTGLVDVLDRVLDKGLVIAGDIRVSLAEVELLT
                     IRIRLLVCSIDKAEQIGLDWWRQDPNLSVAARRLTLENDALREQVRLLEARVDALSAE
                     PREREGRSGRAGKRPGPAGTQTTRE"
     gene            860..2692
                     /locus_tag="VEL74_14420"
     CDS             860..2692
                     /locus_tag="VEL74_14420"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYG63772.1"
                     /translation="MKTMTLCLLLTLLPGLAGGQAAGQEVESYDLGEIAAARRLFEAN
                     LQAIRDKDKDAYLACYLQSHGLVRSGPPGFDLGYAGLAATAGAGWPDHIDAADLRLTP
                     VQPGMVYGTYRYRVRYGANEVAGLSERLFVETDQGWKIALTTAFPALPGLPPPPRILV
                     GATLVDGTGRPPVPDAVVMLREGRIECAGPRAACPIPVGVETVNVTGRWIVPGLVDAH
                     VHFSQTGWADGRPDSLDVRDRHPYEELQGELRRHPERFGRSHLCAGVTAVFDVGGYPW
                     TLTLPAWAEPRSDVARVAAAGPLLSALDHWVNLPGERQFLYLADPDVARRGVQYLAAE
                     GADAVKVWLIPTRERPVPALEPLVTLAGEEAARRKLPLIVHATGLAEAKAALRAGAKL
                     LVHSVWDQPVDQEFLDLAKKDGTVYCPTITVGRGYVRMYESAARDQAPVIDDPNGCVD
                     PTTRARLAETPGLDAGFPAEQIAAMDARIAGQERVMAENLKKVAAAGIPIAMGTDAGN
                     PFTLHGPSVYAEMEAMQAAGLSAMDVLVASTRGGALAMGRQADLGTVEKGKLADLLIV
                     GADPAADIKNLRKVLWVVRGGEMRPIAELAALAAGAGQEREQER"
     gene            2726..3082
                     /locus_tag="VEL74_14425"
     CDS             2726..3082
                     /locus_tag="VEL74_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017464102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HYG63773.1"
                     /translation="MVANVLVGLVALLHLYFLVLEMFLWTKPAGRRAFGLSPELAAQT
                     KSLAANQGLYNGFLAAGLLWGLIRGADGVPVVIFFLACVIVAGVFGAATASRKILWVQ
                     ALPGALALAAVLLARS"
     gene            3091..3951
                     /locus_tag="VEL74_14430"
     CDS             3091..3951
                     /locus_tag="VEL74_14430"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYG63774.1"
                     /translation="MSLTVHPIDTPLGRWTHTEWRPDPGSEMAGLVQHIWHFEGTLSF
                     TRERHFPNGTLELIVQLDDPYRDIHGERTEVCPSVCFTGLQTGPMVIEAPGGFSRVLG
                     VRLHPAGAYALFGRSLAETSGISSVDVRDLAGPAADELAERCHAAASAEERLRAAASW
                     VGERLARSRGVDPAIAWTAAQIERRHGAVSIAALRERAGLTKTRLATAFREQIGVPPK
                     LYARIQRFRHLLTLLHAQAGSLADLSAEAGYYDQPHMNAEFRKLSGVTPGDFLAARRY
                     TSSSLAEPAP"
     gene            4048..4491
                     /locus_tag="VEL74_14435"
     CDS             4048..4491
                     /locus_tag="VEL74_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYG63775.1"
                     /translation="MPTQVIPYLSIQGAASALEFYKKAFGAKEELRLTEPGTGRIGHA
                     EITIGGSRIMLADEYPEYGIVSPKTLGGAGLRICLIVDDVDAVVARAVDAGAKLTMPV
                     RDQFYGDRSGKVEDPFGHTWYIQTHKEDMSAEEMQRRYDEELSAQ"
     gene            4877..5485
                     /locus_tag="VEL74_14440"
     CDS             4877..5485
                     /locus_tag="VEL74_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HYG63776.1"
                     /translation="MAHRIALLVSGASGMLLPRHLLGRLASHPEVERIHLVVSAGASQ
                     VLRHELGPDRTGAADLADAAGLDPAARSKVVVHRDNELDAPIASGSYRLTGTAVLPCS
                     AGTLGALATGSARTLVHRAGAVALKENRPLVLGFRETPYSLVHVENMRRLLYMGAVIL
                     PPVPAFYIGGEDLNRFLDHYALRVMDRLGVSEPGEPGLRWGE"
     gene            5512..6378
                     /locus_tag="VEL74_14445"
     CDS             5512..6378
                     /locus_tag="VEL74_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HYG63777.1"
                     /translation="MRPLRTLSTVLEMIKFEHTLFALPFAFLGMILAAEGGPSWRTVL
                     WIVVAMVGARSAAMGWNRLVDRRIDAANPRTATRALPAGLVSPAAVTLFIVASSALLV
                     VAAWQLNPLALALSPVALAILFLYSYTKRFTWASHLVLGLSLAGAPLGAWIAVRGDVT
                     ATPLILAGSVLLWVAGFDVLYALQDVEFDRRTGLYSIPARFGVVGALWISAGLHALML
                     ALLALLPRVYPPGLGAGFWVGWAGCLALIAYQHWVVRPGDLSRLNAAFFTANGVLSVW
                     LFAAIALDILLP"
     gene            6378..7514
                     /gene="mqnE"
                     /locus_tag="VEL74_14450"
     CDS             6378..7514
                     /gene="mqnE"
                     /locus_tag="VEL74_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HYG63778.1"
                     /translation="MAANLSLIRPEAFPAPLRDIAAKVRDGVRLDEADALTCLTTPHL
                     LHLGRLADTVRRRLHGNVTYFNINRHINPTNVCVYTYNCKFCSFAALPGEDHAWEMSH
                     EDVYKHAADQGGNRVTEFHIVGGLHPDLSLDWYLEMMRGLKQRFPNAHLKAFTAIEVG
                     WFAKREKLSIEEVLRRMRDAGLGSLPGGGAEIFHAEVREIICDGKLDADEWIEVHRVA
                     HKMGIKSNCTMLYGHVERPEHKVDHLLRLRALQDETGGFNAFIPLAYHPENNYMGLTY
                     HTTGGDDLRHIAASRLVLDNIPHIKAYWVMVTPKLAQVALRFGADDMDGTVVEEKIYH
                     MAGAGTSQQLSRSELERIVREAGFVPVERDTLYNPIAPIAPAAA"
     gene            7511..8173
                     /locus_tag="VEL74_14455"
     CDS             7511..8173
                     /locus_tag="VEL74_14455"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HYG63779.1"
                     /translation="MKPEPLGFRYLAVDGPIGVGKTTLVEMLAHRFEGMKILEDVDNP
                     FLGDFYQDRPGAAFQTELYFLLSRYKQQQEITQQNLFDRFLVADYTFQKNRIFAYLNL
                     SDDELMLFDKLYALLEPQIPVPDLIIHLVADVDTCMERIRKRARNVEKTISANYLAEL
                     IDAYNHYYHYYTRSPLLVVDTRHMNFPQRPEDFDELLHRLAKPIKGTEYFQPLGSANP
                     RA"
     gene            8418..9269
                     /gene="panB"
                     /locus_tag="VEL74_14460"
     CDS             8418..9269
                     /gene="panB"
                     /locus_tag="VEL74_14460"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HYG63780.1"
                     /translation="MAGETKSVTVPWVAGAPQRNERVVMVTAYDFPQGRTADAAGADI
                     VLVGDSLAMVVLGHPDTLSVTMDEMLHHVKAVRRGVKRALLVADMPYGSFHLGTDHAV
                     SNAIRFIKEGGAQAVKIEGARPELVAALAQAEVPVMAHLGLTPQSVHRFGGFKVQGRD
                     ADARRLILEAATAVEEAGAFSLVLECVPSDLAAEITRNLSIPTLGIGAGPECGGQVLV
                     YHDLLGLEERIAPRFVRRYAELGLLSREAIERFADDVREGRFPTPEESYGAVTRPAAE
                     AVGKIYG"
     gene            9282..10133
                     /gene="panC"
                     /locus_tag="VEL74_14465"
     CDS             9282..10133
                     /gene="panC"
                     /locus_tag="VEL74_14465"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HYG63781.1"
                     /translation="MITTRTEADLRRKVKDWHQAGLSVGFVPTMGALHAGHLSLVGIA
                     RTHADRVVASVFVNPTQFGPGEDFGRYPRQPEEDAAMLAGAGCDLLFLPEVETMYPPG
                     NTTFVDLGAPSEGLEGHFRPGHFRGVATVVCGLFNLVRPDVAVFGEKDGQQLAVIRQM
                     VRDLHMPVEIVAGPTIREEDGLAMSSRNAYLSPDERRAASVLYRALSAAERAIAAGER
                     RGGEVRRVLHEVLDSEPTARVEYAEVVDAESFRPLETLSGRLVLPLAVRIGGTRLIDN
                     FQLAVEG"
     gene            10159..10560
                     /gene="panD"
                     /locus_tag="VEL74_14470"
     CDS             10159..10560
                     /gene="panD"
                     /locus_tag="VEL74_14470"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HYG63782.1"
                     /translation="MRRTLLKSKIHRATVTGADLHYQGSVKIDPLLMEAADIVEWERV
                     EIYNVTNGERFATYAIPGIPGDGEIVLNGAAAHKVSKGDLVIIATYADFEAQEIRDHV
                     PSLVFVDEANRRAIHPPVVMAPEPELELALG"
     gene            10634..11449
                     /locus_tag="VEL74_14475"
     CDS             10634..11449
                     /locus_tag="VEL74_14475"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HYG63783.1"
                     /translation="MATTLEAATPQTATDVAPLTIAGRTFRSRLFLGTGKYPDFETMA
                     AALDASGTEVVTVALRRVKMDERGRGSLLDFVDQDRYLLLPNTAGCYTADEAIRTARL
                     ARELGGWNWVKLEVIGDQKTLFPDNVELLKATETLVAEGFVVLPYTNDDPVICRKLEE
                     LGAAAVMPLGAPIGSGMGIRNPANLRIILEQANVPVIVDAGVGTASDAAVAMELGAAG
                     VLMNTAVAEAKDPIRMAKAMKLAVEAGWLAAHAGRIPRKLYASASSPMTGLIE"
     gene            11508..11584
                     /locus_tag="VEL74_14480"
     tRNA            11508..11584
                     /locus_tag="VEL74_14480"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11542..11544,aa:Arg,seq:acg)
     gene            11643..11918
                     /locus_tag="VEL74_14485"
     CDS             11643..11918
                     /locus_tag="VEL74_14485"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HYG63784.1"
                     /translation="MRFEWDPEKEVRNLRKHGVSFLEASTVFGDALAATIPDRKHSEL
                     ELRFLTMGMSSGNRLLIVAHTDIEESVRIISARTATAQEKRRYESGT"
     gene            11902..12114
                     /locus_tag="VEL74_14490"
     CDS             11902..12114
                     /locus_tag="VEL74_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016861192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63785.1"
                     /translation="MSQEPEGDDLEPEYDFSGAVRGKYYQRYQQGTNVVLLDPDVAQA
                     FKDSESVNRALRLLLDLARHEVPQSR"
     gene            complement(12179..12829)
                     /locus_tag="VEL74_14495"
     CDS             complement(12179..12829)
                     /locus_tag="VEL74_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63786.1"
                     /translation="MLRFEKSILVGVFSVLAVPGAVWSHGSNPSHLRAELISPYGVTA
                     VVTSTAPGHHTVYGGDLSMDVAVAGGSAGYAVYFDAKAISPATAVRAKVLQGGANAPV
                     FACSTYAQGGYRMQLEVQVLYNGVWTAIGTIWYGHVDGILKNNGDIVTAPTRIAQVSG
                     QYNGSTTCSTNPHLHLELWNYAHYAGYVPRAAQSTFTITDALGCWGGENAADLPCP"
     gene            complement(13071..13394)
                     /locus_tag="VEL74_14500"
     CDS             complement(13071..13394)
                     /locus_tag="VEL74_14500"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HYG63787.1"
                     /translation="MRRTLLDEQLPRDLKAALATVSAVFVHELGWKGTKNGELLRRAA
                     DAGFEVFVTMDRKLRHQQNLRGIPFGIVLVRARTNRWEDLLPLVPVLLVALAEVRPGE
                     IREVG"
     gene            complement(13375..13599)
                     /locus_tag="VEL74_14505"
     CDS             complement(13375..13599)
                     /locus_tag="VEL74_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HYG63788.1"
                     /translation="MSTRQSQVQVDPEVLSGTPVFRGTRVPVQSLFDYLEAGDSLDVF
                     LKQFPSVDRAQAVAVLEEARDRVLNAPHAA"
     gene            complement(13727..14260)
                     /locus_tag="VEL74_14510"
     CDS             complement(13727..14260)
                     /locus_tag="VEL74_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63789.1"
                     /translation="MRSFTDGSSPSAQPRNAFTANFLGRLAEREEGPTAPEADVAGPW
                     RIEEISGDGYGVFRLGESPARGFEPTLSFPAEWLALIAAGLLPGTGRDPMLYLQKDRN
                     PDGTFSITLENGEEVGRSRLFDEKLLDGMNIAIHLLRAPESLANLLDAAGLSPLERCG
                     AILDERAGQRAARAGEK"
     gene            14836..15852
                     /locus_tag="VEL74_14515"
     CDS             14836..15852
                     /locus_tag="VEL74_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008463327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HYG63790.1"
                     /translation="MKAIGLSTSLPIDDPRSLFEFETETPTPGPRDLLVRVRAVSINP
                     VDWKQRQRVAQGTTLPEPRILGYDASGVVEAVGPEVSLFRPGDAVFYAGSMQRQGSNA
                     ELQLVDERIVGRKPATLSDAEAAALPLTSLTAWEAIFDRLRIPEGGGAGKALLIIGGA
                     GGVGSIAIQIAKQLTGLEVIATASREESARWCRELGADQIADHRDLVTSVRGLGRQFV
                     EYILCTNDFEGHWRSLGELIAPQGAICSILGARQLGDVGPLMAKSATLAWELMFTRSS
                     FGTEDMAEQHRILNRVADLVDAGRIRSTLTRTLEGFTAANHRQAHQLVEAGRMVGKVV
                     IAYS"
     gene            complement(16078..16320)
                     /locus_tag="VEL74_14520"
     CDS             complement(16078..16320)
                     /locus_tag="VEL74_14520"
                     /inference="COORDINATES: protein motif:HMM:NF021009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H-type lectin domain-containing protein"
                     /protein_id="HYG63791.1"
                     /translation="MAQLIQTGVAQKAAPGLLTINFPQPFSTIPVVVISPYWQNAGQQ
                     SGNIDTIDTISPASFTIASQNNSPIYYITWIAVGED"
     gene            16846..17316
                     /locus_tag="VEL74_14525"
     CDS             16846..17316
                     /locus_tag="VEL74_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63792.1"
                     /translation="MSSRYFWDIGFDLTSPNLRYGLVEGYSLSTESGTSATLTTSPVF
                     HPGDQVDFNVFNLSPSTCTNVVSSLTLTIDFGERCPFVETPLQFTIPGQGAGEPGNSS
                     AFPGRQYPHWMQWPFIVSKATGRYCFKASLTVNTANGPVSFSLPDPEMIVGSDG"
     gene            complement(17327..>19139)
                     /locus_tag="VEL74_14530"
     CDS             complement(17327..>19139)
                     /locus_tag="VEL74_14530"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG63793.1"
                     /translation="ETTLSRPGTIVGTSYAMSPEQAMGLPLDARSDLFSLGSLLYEMV
                     TGVAPFRAETAQATLARVCSFRQCPASDIRPDVPRELSDLIDRLLEKDPAYRPGSAAE
                     VATDLDRIATKREVFPAGAQVSLEESETCGQETDLEMPAWQIQASSSFERTAGQPLPD
                     GLPAAAEPAQLSGSLAGQEPPLRRIRSLRTAIGIALLILVMIVAAYSLRLPAVQPTPH
                     SLYQEGLTYLDRPDLKGNLDQAIDSFQKVLARDQTHAAAQAALARAYWFKFRSDSRDP
                     MLLTWALSNAERAVALDPYLAAARVSLGLALMSDGRTDEAMRHIERALVLEPANGDAH
                     YAAGRIHESQGRFKEAEAAYKKAAEVQPHRLNFDALGTLYYNMGRMEEAIAASRRSIE
                     LAPDGFIGYRNLGAAYHAQGDLAEAASQFQKALQIRPDATVYSNLGTVHFSQGFYQLS
                     AEAFEKSLEMPGGANNYKVWGNLGDAYRWTPDQKERSRDAYLRGIQLVQEQLRSTPGD
                     VTLRSSLALYLAKRGDLAEALAELHKLRDLPGKDAKAWLRMVVAYEVSAQRSEALAAL
                     GNALRAGASIQEVKDDPELLELRADARYHRLIAELSK"
BASE COUNT         3188 a         6675 c         6185 g         3091 t
ORIGIN      
        1 cggcgttctc cgccatcagc tcttcggggg accggctcgt ctcgagcgcc ggacgggaga
       61 cggtgggcac ctgggcgacg gcttccgagt atttgatgta ggtatcgatc gatgcgacga
      121 cgacgcgggc ctcgacggtg agcaggtcga ttcccaccag cgagacccgt acccaggcgt
      181 cgatcacgat gcccttgtcc aggacccggt ccaggacgtc gatgagactg gtaccaccgg
      241 aggctctttc aacgggcatt ttctactccc tggttgtctg ggtaccggcg gggcccggcc
      301 gcttaccggc tcggccgctc ctcccctccc gctcccgagg ctccgcgctc aaggcgtcca
      361 cccgcgcctc gagaagacgg acctgctcgc gcagggcgtc gttctcgagc gtcagacggc
      421 gggccgccac ggaaagattg gggtcctggc gccaccagtc gaggccgatc tgctccgcct
      481 tgtcgatgga gcagacgagc agccggatgc ggatggtgag cagctccacc tccgccaggg
      541 agacgcggat gtccccggcg atgaccaggc ctttgtccag cacccggtcg aggacgtcga
      601 ccaggccggt ggaccggcga tagctcggct cgatggagga tggcatcttt ttctgactcg
      661 tcagctccag taggccagca gtgacagacg cgcaggccgc gatcctacgc caaccccgca
      721 gacggtgcaa tccaccagag gacgacccgg gggtgagccg ttcatcgcct gcaagatcct
      781 ggccggaaac ccgcggcccc accggctccg ccacgcccta cactactccc tgccctacac
      841 cgtaccgagg aaccgacgaa tgaagacgat gacgctctgc ctgctcctga ccctgctgcc
      901 aggcctcgcc ggaggacaag ccgccgggca ggaagtcgaa agctacgacc tcggcgagat
      961 cgccgccgcc cgccgcctgt tcgaggccaa cctccaggcg atccgcgaca aggacaaaga
     1021 cgcctacctc gcctgctacc tccagtcgca cgggctggtg cgcagcggcc cgcccggctt
     1081 cgacctcggc tatgccggcc tggcggcgac cgcgggcgcg gggtggcccg accacatcga
     1141 cgccgccgac ctgcgcctga cgccggtgca gccagggatg gtctacggca cctaccggta
     1201 ccgggtgcgc tacggcgcca acgaggtggc gggcctgtcg gagcggctct tcgtcgagac
     1261 ggaccagggc tggaagatcg ccctgaccac cgccttcccg gccctacccg gcctgcctcc
     1321 gcccccccgc atcctggtcg gcgccaccct ggtggacggc accggccggc cgccggtccc
     1381 cgatgccgtg gtgatgctgc gcgaagggcg gatcgagtgc gccggcccgc gcgccgcctg
     1441 cccgatcccg gtcggcgtcg agaccgtcaa cgtcaccggc cggtggatcg tgcccggtct
     1501 ggtggacgcc cacgtccact tttcgcagac aggctgggcg gacggacggc ccgactccct
     1561 ggacgtccgc gaccggcacc cctacgaaga gctgcagggt gagctccgcc gccacccgga
     1621 gcgcttcggc cgctcccacc tatgcgccgg cgtcaccgcg gtcttcgacg tcggcggcta
     1681 cccctggacg ctgaccctcc ccgcctgggc cgagccgcgc tccgacgtgg cccgggtggc
     1741 ggccgccggc ccgctgctct ccgccctgga ccactgggtc aacctgccgg gcgagcgcca
     1801 gttcctctac ctcgccgacc cggacgtggc gcgccggggg gtccagtacc tcgcggccga
     1861 aggcgctgac gccgtcaagg tctggctcat ccccacccgc gagcggccgg tgccggccct
     1921 ggagcccctg gtcaccctgg ccggggagga ggcggcccgg aggaagctgc cgctgatcgt
     1981 ccacgccacc ggcctcgcgg aggccaaggc ggcgctccgc gccggcgcca agctcctcgt
     2041 ccacagcgtc tgggaccagc cggtcgacca ggagttcctc gacctggcga agaaggacgg
     2101 cacggtctat tgcccgacga tcaccgtcgg ccggggctac gtccgcatgt acgagagcgc
     2161 ggcgcgggac caggcgccgg tgatcgacga ccccaacggc tgcgtcgatc ccaccacccg
     2221 tgcccgtctc gccgagacgc cggggctgga tgccggtttc ccggcggagc agatcgccgc
     2281 catggacgcc cgcatcgccg gccaggagcg ggtgatggcc gagaacctga agaaggtggc
     2341 ggcggcgggc atccccatcg ccatggggac cgacgcgggc aacccgttca ccctccacgg
     2401 cccgtcggtc tacgccgaga tggaggcgat gcaggccgcc ggcctctcgg ccatggacgt
     2461 gctggtcgcc tccacccgcg gcggcgccct tgccatgggc cggcaggccg acctcgggac
     2521 ggtcgaaaag ggcaagctcg ccgacctcct gatcgtgggg gccgacccgg cggccgacat
     2581 caagaacctg cgcaaggtcc tctgggtggt gcggggaggg gagatgcgcc cgatcgccga
     2641 gctggcggcg ctcgccgccg gcgcgggcca ggagagagag caggagagat aggatagagg
     2701 ctcaagggag ggtcaagaga tgaacctggt ggcgaatgtc ctggtcgggc tcgtggcgtt
     2761 gctgcatctc tacttcctgg tcctcgagat gttcctctgg acgaagccgg ccggccgccg
     2821 cgccttcggc ctctcgcccg agctcgcggc ccagaccaag tcgctggcgg ccaaccaggg
     2881 cctctacaac ggcttcctcg ccgccggcct cctctggggc ctgatccggg gcgccgacgg
     2941 ggtgccggtc gtgatcttct tcctggcctg cgtcatcgtc gccggagtgt tcggcgcggc
     3001 gaccgcgagc cgcaagatcc tctgggtcca ggcgttgccg ggcgccctcg ccctggccgc
     3061 ggtcctgctc gcccgctcct agaaaaagga atgagcctca ccgtccaccc gatcgacacc
     3121 cctctcggcc gctggacgca cacggagtgg cggcccgatc cggggtcgga gatggccggg
     3181 ctggtgcagc acatctggca tttcgagggg accctgagct tcacccgcga gcggcacttc
     3241 cccaacggca cgctggagct gatcgtccag ttggacgatc cctaccggga tattcacggg
     3301 gagcggaccg aggtctgccc gagcgtctgc ttcaccgggc tccagaccgg ccccatggtc
     3361 atcgaagccc cgggaggctt cagccgcgtc ctcggggtgc ggctccatcc ggccggggcc
     3421 tatgccctct tcggccgctc cctggcggag acgagcggga tctccagcgt cgacgtccgc
     3481 gacctggcgg gccccgcggc ggacgagctc gccgagcgct gccacgccgc cgcctcggcc
     3541 gaggagcggc tgcgcgccgc ggcgtcctgg gtcggcgagc gcctcgcgcg ctcccgggga
     3601 gtggacccgg ccatcgcctg gacggcggcc cagatcgagc gccggcacgg cgcggtctcg
     3661 atcgccgccc tgcgcgagcg ggccggcctg accaagacgc ggctcgccac cgccttccgc
     3721 gagcagatcg gcgtgccgcc gaagctctac gcccgcatcc agcgcttccg gcatctcctg
     3781 acgctgctgc acgcccaggc cggctctctc gccgacctgt ccgccgaggc cggctattac
     3841 gatcagccgc acatgaacgc ggagttccgg aagctgagcg gcgtcacccc cggcgacttc
     3901 ctggccgccc gccgctacac cagctccagc ctggccgaac cggccccctg agccggactt
     3961 ttgtccaaga cgccgggccg gccaggtcct atcgtgtctc ccaggcggtc ccaaggacgg
     4021 ggccgcggac cttccaagga ggtgaccatg cccacccagg tgattcccta tctctccatc
     4081 cagggcgccg cgagcgccct cgagttctac aagaaggcct tcggcgccaa ggaggagctg
     4141 cgcctcaccg agcccggcac cggccggatc gggcacgccg agatcaccat cggcggatcg
     4201 cggatcatgc tggccgacga gtaccccgaa tacggcatcg tcagcccgaa gaccctcggc
     4261 ggcgcgggcc tccggatctg cctgatcgtg gacgacgtcg acgccgtggt cgcccgagcc
     4321 gtcgacgccg gcgcgaagct cacgatgccg gtacgggacc agttctacgg cgaccgctcc
     4381 ggcaaggtgg aggatccttt cggccacacc tggtacatcc agacccacaa ggaggacatg
     4441 tccgccgagg agatgcagcg ccgctatgac gaggagctga gcgcccaata ggcgccgcca
     4501 tccccgaaaa acgaagaccc tacccggaga gacgacgacc gtacccgccg aaacaagacc
     4561 atacccgggc agacgggcat cgtccccgcg ggtacggcca ccctaccctg ggtacgaagg
     4621 cgctccccgc gggtacggcg atcgccgcgt gggtatggtc atcctccccc gggggatgac
     4681 agccctacct cgggtaggac ggaccttttc cggggtatgg cggacatccc tcgcggggca
     4741 acggctgccc cggggaagtg gcgacgcgtc aagagggaaa tggtggattt gcggggagat
     4801 gcgccccctc cccctgggcc gttagcccca gcgggtgggg ggccggaccg ggttttggca
     4861 tacgatccct cctcgcatgg cccaccgcat cgccctcctg gtctccggcg cctccggcat
     4921 gctcctcccc cgccacctgc tggggaggct cgcctcccat ccagaggtgg agcggatcca
     4981 cctggtggtc tcggcgggag cgagccaggt gctgcgccac gagctcggac cggaccgcac
     5041 cggcgccgcc gacctcgccg atgccgccgg cctcgacccc gccgcccggt ccaaggtcgt
     5101 ggtccaccgg gacaacgagc tggacgcccc catcgcctcg ggctcctacc ggctgacggg
     5161 caccgctgtg ctgccctgca gcgccggcac cctgggagcg ctggccaccg gctcggcccg
     5221 gaccctcgtc caccgcgccg gcgccgtggc cctcaaggag aaccggcccc tggtcctggg
     5281 ctttcgggag accccctaca gcctggtcca tgtggagaac atgcgccgcc tcctctacat
     5341 gggagccgtg atcctccccc cggtccccgc cttctatata ggaggcgagg acctcaaccg
     5401 cttcctcgac cactacgccc tgcgggtcat ggaccgcctc ggcgtgagcg agcccggcga
     5461 gcccgggctg cggtgggggg agtagagccg cttcagatag gctagagacc catgcgcccc
     5521 ctccgcaccc tctcgaccgt gctcgagatg atcaagttcg agcacacgct gttcgcgctg
     5581 ccgttcgcgt tcctggggat gatcctggcc gccgaggggg ggccgtcgtg gcggacggtg
     5641 ctctggatcg tcgtcgccat ggtcggggcg cgcagcgccg ccatggggtg gaaccgcctg
     5701 gtggaccggc ggatcgacgc cgccaacccg cggacggcga cccgcgccct ccccgccggc
     5761 ctggtgagcc ccgcggccgt gaccctgttc atcgtcgcca gctcggccct gctggtcgtc
     5821 gccgcctggc agctcaaccc gctggcgctg gccctctctc cggtggccct ggcgatcctc
     5881 ttcctctatt cctataccaa gcggttcacc tgggcctctc acctggtgct cggactctcc
     5941 ctggccggcg cccccctggg ggcctggatc gcggtgcgcg gcgacgtcac ggcgactccc
     6001 ctgatcctcg ccggctccgt gctcctctgg gtggccggct tcgacgtcct ctatgccctc
     6061 caggacgtgg agttcgaccg ccgcaccggc ctctactcca tcccggcgcg cttcggcgtc
     6121 gtcggcgcgc tctggatctc ggcggggctc cacgccctga tgctcgccct cctcgccctg
     6181 ctgccgcggg tctacccgcc cggcctcggc gccggcttct gggtaggctg ggccggatgc
     6241 ctcgccctca tcgcctacca gcactgggtc gtgcgccccg gcgacctgtc gcgcctcaac
     6301 gcggctttct tcaccgccaa cggcgtcctc tcggtctggc tcttcgcggc gatcgccctt
     6361 gatatactcc tgccgtgatg gcagcgaatc tctctcttat ccgtcccgaa gcctttcccg
     6421 cacccctgcg cgacatcgcc gccaaggtgc gtgacggcgt ccgcctcgac gaggccgacg
     6481 ccctgacctg cctgacgacg ccgcacctgc tccacctcgg tcgcctggcc gacacggtgc
     6541 gccggcgcct ccatggcaac gtcacctact tcaacatcaa ccggcacatc aaccccacga
     6601 acgtctgcgt ctacacctac aactgcaagt tctgcagctt cgcggccctt cccggcgagg
     6661 accacgcctg ggagatgagc cacgaggacg tgtacaagca cgcggccgac caggggggca
     6721 accgggtcac cgagttccac atcgtcggcg ggctccatcc ggacctctcc ctcgactggt
     6781 acctggagat gatgcgcggc ttgaagcagc gcttccccaa cgcccacctc aaggccttca
     6841 cggccatcga ggtcggctgg ttcgccaagc gcgagaagct gtccatcgag gaggtgctgc
     6901 gccggatgcg cgacgccggc ctgggatcgc tccccggcgg cggggcggag atcttccacg
     6961 ccgaggtgcg ggagatcatc tgcgacggca agctcgacgc cgacgagtgg atcgaggtcc
     7021 accgcgtcgc ccacaagatg gggatcaaga gcaactgcac catgctctac ggccacgtcg
     7081 agcggccgga gcacaaggtg gaccacctgc tgcgcctgcg cgccctccag gacgagaccg
     7141 gcggcttcaa cgccttcatc cccctggcct accaccccga gaacaactac atggggctga
     7201 cgtaccacac caccggcggc gacgacctgc ggcacatcgc ggcctcgcgc ctggtgctgg
     7261 acaacatccc gcacatcaag gcctactggg tgatggtcac cccgaagctg gcgcaggtgg
     7321 ccctgcgctt cggggcggac gacatggacg gcaccgtggt ggaggagaag atctaccaca
     7381 tggccggcgc cggcacgagc cagcagctct cccgctccga gctcgagcgg atcgtgcgcg
     7441 aggccgggtt cgtcccggtc gagcgcgaca cgctctacaa cccgatcgcg ccgatcgcgc
     7501 cggctgctgc gtgaagcccg agcccctcgg tttccgctac ctggcggtgg acggccccat
     7561 cggggtcggc aagactaccc tggtggagat gctcgcccac cgcttcgagg ggatgaagat
     7621 cctcgaggac gtggacaacc cgttcctcgg cgacttctac caggaccgcc ccggcgccgc
     7681 cttccagacc gagctctact tcctgctgtc gcgctacaag cagcagcagg agatcaccca
     7741 gcagaacctg ttcgaccgct tcctggtggc cgactacacg ttccagaaga accgcatctt
     7801 cgcctacctc aacctgtccg acgacgaatt gatgctgttc gacaagctct atgccctgct
     7861 ggagccccag attccggtac cggacctgat catccatctg gtggccgacg tcgacacctg
     7921 catggagcgg atccgcaagc gcgcacgcaa cgtcgagaag acgatttcgg cgaattacct
     7981 cgccgagttg atcgacgcct acaatcacta ctatcattac tacacgcggt cgccgctgct
     8041 ggtggtagac acccggcaca tgaacttccc ccagcggccc gaggacttcg acgagcttct
     8101 gcaccggctc gccaagccca tcaaggggac cgagtacttt cagccgcttg gatccgccaa
     8161 tccccgcgcc taggcatctc gcctagtaga cgccgggagc ggaccgagac gggagcagcc
     8221 cagcgccgcc cggcggcgtg gcccacggca gtcgagggcc cgatccccca ggatctttaa
     8281 cgagggaggg gacggacacc tcgcgcagct ccggcaggcc gcgcacatac gaccgtgctc
     8341 gcccaaggcc cttccgtctc cccaggcgga agggtttttc gcttctccgg gttttgaatc
     8401 ccggaaagga ggctgcgatg gcgggcgaga cgaaatccgt gaccgtgccc tgggttgccg
     8461 gggctcctca gcgcaacgag cgcgtggtga tggtcaccgc gtacgacttc ccccaggggc
     8521 gcaccgccga cgcggccggc gccgacattg tcctggtggg cgactccctc gccatggtgg
     8581 tcctcggcca tcccgacacc ctgtcggtga ccatggacga gatgctccac cacgtgaagg
     8641 cggtgcggcg cggcgtcaag cgcgccctgc tggtcgccga catgccttac ggctcgtttc
     8701 acctcggcac cgaccacgcc gtcagcaacg ccatccgctt catcaaggaa ggcggcgcgc
     8761 aggcggtcaa gatcgagggc gcccggccgg agctggtggc cgccctcgcc caggccgagg
     8821 tgccggtgat ggcccacctc ggcctcacgc cgcagtcggt ccaccgcttc ggcggcttca
     8881 aggtccaggg gcgggacgcg gatgcccggc gcctgatcct cgaggcggcc acggccgtcg
     8941 aggaggcggg cgccttctcg ctcgtcctcg agtgcgtgcc gagcgacctg gcggcggaga
     9001 tcacccgcaa cctctccatc cccaccctcg gcatcggcgc cggcccggag tgcggcggcc
     9061 aggtgctggt ctaccacgac ctgctcggcc tcgaagagcg gatcgcgccg cgcttcgtgc
     9121 ggcgctacgc cgagctcggc ctgctctccc gcgaagccat cgagcgcttc gccgacgacg
     9181 tccgcgaagg acgtttcccc acgccggagg agagctacgg cgcggtgacc cggcccgccg
     9241 ccgaagcggt gggcaagatc tacggctgag aggcgagccc attgatcacg acccgtaccg
     9301 aggcggacct ccgccgcaag gtcaaggact ggcatcaggc cggcctgtcc gtggggttcg
     9361 tccccaccat gggcgcgctc cacgccggcc acctgtcgct ggtcggcatc gcccggacgc
     9421 acgccgaccg ggtggtggcg tcggtcttcg tcaacccgac ccagttcggg cccggcgagg
     9481 acttcggccg ctacccgcgc cagcccgagg aggacgccgc gatgctggcg ggcgccggct
     9541 gcgacctgtt gttcctgccc gaggtggaga cgatgtatcc gccggggaac accacgttcg
     9601 tcgacctcgg cgcgccgtcc gagggcctcg aggggcactt ccggcccggc cacttccggg
     9661 gcgtcgccac cgtcgtctgc ggcctcttca acctggtgcg cccggacgtg gcggtcttcg
     9721 gcgagaagga cggccagcag ctcgccgtga tccgccagat ggtgcgcgac ctccacatgc
     9781 cggtggagat cgtcgccggc cccaccatcc gcgaggagga cggcctggcg atgtcgtcgc
     9841 gcaacgccta cctgtcgccg gacgagcggc gcgccgcaag cgtcctctac cgggcgctgt
     9901 cggccgccga gcgggccatc gcggccggcg agcggcgggg cggcgaggtg cggcgggtgc
     9961 tacatgaagt cctggattcc gagcccacag cccgggtgga gtacgccgag gtcgtggacg
    10021 ccgaaagctt ccggccgctg gagaccctct ccggccggct ggtcctcccc ctggccgtcc
    10081 ggatcggggg gacgcgtttg atcgacaact tccaactggc ggtcgaaggc tgacccggcc
    10141 gccggggaga ttacggatat gcgacgcacc cttctcaagt ccaagatcca ccgggcgacg
    10201 gtgaccggtg ccgacctcca ctaccagggc tcggtgaaga tcgatccgct cctgatggaa
    10261 gccgcggaca tcgtcgagtg ggagcgggtg gagatctaca acgtcaccaa cggcgagcgc
    10321 ttcgccacct atgcgatccc ggggattccg ggcgacgggg agatcgtgct caacggcgcg
    10381 gcggcccaca aggtgagcaa gggcgacctc gtcatcatcg ccacctatgc cgacttcgag
    10441 gcgcaggaga tccgcgacca cgtcccgtcg ctggtcttcg tcgacgaagc caaccgccgg
    10501 gcgatccacc cgccggtggt gatggccccg gagcccgagc tggagctggc gcttggctga
    10561 agggatcccg gccccacccc acggggggag ggccgggaat cccccatcct ctagaatcgt
    10621 tgcaggagga gccatggcca ccaccctcga agccgccacc ccgcagaccg ccaccgacgt
    10681 cgcgccgctc accatcgcgg gccggacctt ccgttcgcgc ctgttcctgg ggacgggcaa
    10741 gtaccccgac ttcgagacca tggcggcggc cctggacgcc tccggcaccg aggtggtcac
    10801 ggtggcactg cggcgggtga agatggacga gcggggccgc gggtcgctcc tcgacttcgt
    10861 cgaccaggac cgttacctgc tcctgcccaa caccgccggc tgctacacgg cggacgaggc
    10921 catccgcacc gcccgcctgg cgcgggagct ggggggctgg aactgggtga agctcgaggt
    10981 gatcggcgac cagaagacgc tcttcccgga caacgtcgag ctgctcaagg cgacggagac
    11041 gctggtggcc gagggcttcg tggtgctgcc ctacaccaac gacgacccgg tgatctgccg
    11101 caagctcgag gagctgggag cggcggcggt gatgcctctg ggcgccccca tcggctcggg
    11161 gatgggcatc cgcaatcccg ccaacctccg gatcatcctg gagcaggcca acgtcccggt
    11221 gatcgtggac gcgggcgtgg gcacggcaag cgacgccgcc gtcgccatgg agctaggcgc
    11281 cgccggcgtc ctgatgaaca ccgccgtggc cgaagcgaag gacccgatcc ggatggcgaa
    11341 ggcgatgaag ctggccgtcg aagccggctg gctcgccgcc cacgccggcc gcatcccgag
    11401 gaagctctac gcctccgcct cctcgccgat gaccggcctc atcgagtagg gcctacgcag
    11461 gagaccccgg cctgctctag aatccgggtc cgcacgagac ccaccgtgca gccatagctc
    11521 aactggatag agcgacagac tacggatctg taggttgggg gttcgactcc tcctggctgc
    11581 accagctttt cctcctccgc aagcagggcc cacactggct cctgggccgg tatacttcga
    11641 gagtgagatt cgaatgggac ccggagaagg aggtgcgcaa ccttcggaag cacggggtct
    11701 cgttcctgga ggcgagcact gtgttcggcg atgcgttggc cgcaacgatc ccggaccgga
    11761 agcattccga gcttgagctc cgcttcctca cgatgggcat gtcctcgggg aaccggctgc
    11821 tgatcgtagc tcacaccgat atcgaggaga gcgttcggat catcagcgcg aggacggcaa
    11881 cggcgcagga gaaaaggcgt tatgagtcag gaacctgaag gcgacgatct cgaaccggag
    11941 tacgacttct cgggagcggt gcgtggcaag tactaccagc gctaccagca gggtacgaac
    12001 gtggtcctgc tcgatcccga cgtggctcag gccttcaagg actccgagtc agtgaaccgg
    12061 gctctccgcc tcctcctcga cctggcccgg catgaggtcc cccagagccg gtgagccccg
    12121 gcgcaagagg agcggccccc gataaggggc cgctcacacg gaagcgaact cgttcgactc
    12181 agggacaggg aagatccgcg gcgttctctc ccccccagca ccccagggca tcagtgatgg
    12241 tgaaggtact ctgcgccgct cgcggaacat agccagcgta gtgcgcgtaa ttccagagct
    12301 ccaagtggag gtgaggattg gttgaacaag tagtggagcc attatattgg ccgctcacct
    12361 gagcgattcg ggtcggcgct gtcacaatgt ctccgttgtt cttgaggatg ccgtcgacat
    12421 gcccatacca gatggtgcca atggccgtcc agaccccatt gtaaagaacc tggacttcga
    12481 gctgcatccg ataaccaccc tgcgcatagg ttgaacaagc aaatactggc gcgttcgccc
    12541 ctccctgcag caccttcgct cgaactgccg tggccgggct gatggccttg gcgtcgaaat
    12601 aaactgcata tccagcgcta ccccctgcca ccgcaacatc catcgacaga tcaccgccgt
    12661 agacggtgtg gtggcccgga gcagtagagg ttacgacggc agtcactcca tagggactaa
    12721 taagttccgc cctcaggtga gaggggttac tgccatggga ccacacagca ccgggtacgg
    12781 cgaggacaga gaacacacct accaaaatgg acttctcgaa tctaagcact tcgtctcctt
    12841 gctcacagtt gcaaacctcg tcaggataag cagccccggg gctacccgac tttctattag
    12901 aatactctaa cattcatcgg gaagatggtc aagctggccc ccgctcacct gggggaatcg
    12961 gctcgccaga gaggaggccg gcggcgtcca aggaggttgg ccagcgactc cggggctctc
    13021 agtaggggat ggccatgttc ataccgtcca gcagctttcg tcgaagagtc tcagcccacc
    13081 tcccggatct cgcccggtct cacttcggcg agagccacca agagcactgg cacgagtggg
    13141 agcagatcct cccatcgatt cgtgcgcgca cggaccagca cgataccgaa aggtatccca
    13201 cgcaggttct gctgatggcg cagctttcgg tccatggtga cgaagacctc gaagcctgcg
    13261 tcggcggccc ggcggagcag ttccccattc ttcgtcccct tccagcccag ctcatgaaca
    13321 aagacggcgc tgaccgtggc cagcgccgcc ttgagatcac gaggcaactg ttcgtcaagc
    13381 agcgtgcggc gcattcaata ccctgtcccg agcttcctcc agcacggcga ccgcctgggc
    13441 gcggtccaca gagggaaact gcttcagaaa cacgtcgagc gaatcccccg cctcgagata
    13501 gtcgaacaga ctctggaccg gaacccgcgt accgcggaac accggggtac cgctgagaac
    13561 ctcggggtct acctgaacct ggctctggcg tgtcgacatc ggctcacctc cggacctgcg
    13621 gtccttctcc agtatatcca acacgcctcg tctcgccccc taaagcgaag cgccaccggc
    13681 ggagtctcct ccgccgacgg cgctcccaat ctatataaga aagaccctac ttctcccccg
    13741 cccgcgccgc ccgctgcccc gcccgctcat cgagaatcgc cccgcacctc tccagcggcg
    13801 agaggccggc ggcgtccagg aggttggcga gcgactccgg ggctctcagc aggtggatgg
    13861 cgatgttcat gccgtccagc agcttctcgt cgaagagccg ggagcggccc acctcctcgc
    13921 cgttctccag ggtgatggag aaggtgccgt cggggttgcg gtccttctgg aggtagagca
    13981 tcgggtcgcg gccggtgccg gggaggaggc cggcggcgat cagggccagc cattcggcgg
    14041 ggaaggacaa cgtcggctcg aagccgcggg ccgggctctc tccgagccgg aacaccccgt
    14101 atccatcccc ggagatctcc tcgatccgcc agggaccagc gacgtcggcc tccggcgcgg
    14161 tcgggccctc ctccctctcg gccagccggc ccaggaagtt ggcggtgaag gcgttgcggg
    14221 gttgagcgga cggactgcta ccatcggtga agctgcgcat aaggggtctc tcccttcgtg
    14281 tgcggctagg ctcgggcggg aggccttaac tccctcccgg gccgctgcca cacggtgttt
    14341 gtggaccgcg gagccggcac catgccggaa cacgggtgct gcgtactacc tgattccaga
    14401 atcctagaac gaagacaggg cagaagtcaa acccttgggg ggattttctt cccgcctccc
    14461 tcggttctgt ttcccccagc agatctgaga cgcatctaaa tcctcctcag gaggatgggc
    14521 aggccatcca ctcgcccatt acgactccat tgacgagcca cgccttctgt cgcacaatct
    14581 acgcacggct gatgtcatta gcgcagccgc ggcgtccaga agcgtccgga gctttcggat
    14641 ggcatccagg ccgccgctct ttgacaactg aatcttgaag ggggcgctgc cgtgtccagg
    14701 gccgcgcccc ccgaggctcc ctctgtccgg caggaggatg ccgggagacg gaggtccaat
    14761 ccctccgcct tccggccggt attggctgct ccactcctga taccgtctca cccggacaga
    14821 agaagggagc ttcccatgaa agccatcggc ctgagcacct ccttgcctat cgacgatccc
    14881 cggagcctgt tcgagttcga gacggagacg cccacgccgg ggccgcgcga ccttctcgtg
    14941 cgggtgcgag cggtctcgat caatccggtg gactggaaac agcggcagcg ggtggctcag
    15001 gggaccacgc tgccggagcc gcgcatcctt gggtacgacg cgtccggggt ggtggaggcc
    15061 gtggggccgg aggtgagcct gttccggccg ggggacgcgg tgttctatgc cgggagcatg
    15121 cagcggcagg ggagcaacgc ggagctccaa ctcgtcgacg agcggatcgt gggccgcaaa
    15181 cccgcaacgc tctccgatgc ggaggcggcg gcgctgcctc tgacctcgct caccgcgtgg
    15241 gaggcgatct tcgaccgctt gcggattccg gagggcggcg gcgccggcaa ggcgctgctg
    15301 atcatcggcg gcgccggcgg tgtgggctcg atcgctatcc agatcgccaa gcagctcacg
    15361 gggctggagg tcatcgccac ggcctcacgg gaggagtccg cgcgctggtg ccgggagctg
    15421 ggagcggacc agatcgcgga ccatcgcgac ttggtgacct cagtgcgcgg cctcggacgg
    15481 caattcgtcg agtacatcct ctgcaccaac gacttcgaag ggcattggcg ctcgttgggc
    15541 gagctgatcg cgccgcaggg ggccatctgc tcgatcctcg gcgcccgcca gctcggcgac
    15601 gtcgggcctc tcatggccaa gagcgccacg ctggcctggg agctcatgtt cacccgctcc
    15661 tccttcggca ccgaggacat ggccgagcag caccgcatcc tcaaccgcgt ggccgacctg
    15721 gtggacgccg gccggatccg ctccacgctg actcggaccc tggagggctt cacggccgcc
    15781 aaccaccgac aggctcacca gttggtagaa gccggccgga tggttggcaa ggtggtgatc
    15841 gcgtactcat gagtcggcga caccccggcc tgctctaaaa tccgaggtcc gcctcctggc
    15901 tgcaccacct ccctcctttg agcagaggcc atccaggccg gctcccgggc cggcatactt
    15961 cgagagagat tcgttttaaa tgggtttcgt tcggctgtct cggtcaagcg ggggagaaag
    16021 ttcccccgcc ttcccgatca tgattctcta cttcaaggat atctcatcct cgcgaggtca
    16081 gtcttctccg actgcaatcc aggtaatgta gtagatgggg gagttgttct ggctggcgat
    16141 cgtgaagctc gcaggtgaga tggtgtcgat cgtgtcgatg ttgcctgact gctgaccagc
    16201 attctgccaa taaggcgaga tgacaacgac cggaatcgtg ctgaacggtt gcggaaagtt
    16261 gatcgtcaac aaccccggag cagccttctg agccactccc gtctggatta actgggccat
    16321 gaatgttctc cttgcgcccg aggcgcgttc tggactttag gaccactttc ggggtggtac
    16381 cctggtgagg ctctcgcaac cgagagccgt tcgatcgact gacacccatg ggatgctgtt
    16441 ctttatcgcc ggctgacgag gcaacaaagc aagggatgtg ccagcggccc cgcgaattgg
    16501 aagaaggctg ctacggagag cgaacccgat gagataggtt ttgtgtggat ccaatcgtcc
    16561 acacacgtgt agacaaaagg acacttcgcc gccaaccacc ggcaggccca ccagttggtg
    16621 gaagcaggcc agatgatcgg caaggtggtg atcgcgtact cgtgagtcga cggcggaggg
    16681 catgcagcca tggctcaaga ggtctgcacc cgtgaaatcc ctgacacccc agacaactct
    16741 gacagcctcg acaacctgag aacggagtaa ttttctctat ccccccggcc cttcacatgg
    16801 cacgagcatt gctccctctg agcggaaatc acaataggag ggtttatgag cagcagatat
    16861 ttctgggata tcggattcga cctcaccagc cccaatttgc gctatggact tgtcgaaggc
    16921 tactctctct ctacggagag tggcacctcg gccactctga ccaccagccc agtcttccac
    16981 ccaggagatc aagtcgactt caatgtgttc aacctttcac catcaacctg cacaaacgtc
    17041 gtaagcagcc tgacactaac gatcgatttt ggcgaacgtt gtcccttcgt ggaaacgcct
    17101 ttacaattta caatcccagg ccagggagca ggggaaccgg ggaatagcag cgccttcccc
    17161 ggtcgtcaat atccgcactg gatgcagtgg ccttttatag tatcaaaagc tacgggcagg
    17221 tattgcttca aggcctccct gactgtcaac acagcgaatg gccccgtcag tttctctctc
    17281 ccagatcccg agatgatcgt cggcagcgac ggctgatacc ccaagattac ttggagagct
    17341 ccgcgatcag gcggtgatat cgggcatccg cccggagctc cagcagttcc gggtcgtcct
    17401 tgacctcctg aatggaagcg ccggctcgca gcgcattccc gagcgctgcg agcgcctcgc
    17461 tccgctgagc cgagacctca taggcaacta ccatacgcaa ccaggccttc gcgtccttgc
    17521 cggggagatc tcggagctta tgcagctccg ccaacgcctc ggcgaggtct ccgcgctttg
    17581 cgaggtagag cgcgaggcta ctgcggaggg tcacatcgcc cggcgttgaa cgcaattgct
    17641 cctggacgag ttggatacct ctcaggtaag cgtcacgcga cctctccttc tggtcagggg
    17701 tccagcggta tgcgtcgccc aggttgcccc agaccttgta gttgttggcg cctcccggca
    17761 tctcgaggga cttctcgaaa gcctcggcag acaactggta gaagccttga gagaaatgga
    17821 ccgtgccgag gttcgagtag acagtcgcgt caggccggat ctggagggcc ttctggaact
    17881 gggaagcggc ctctgcgagg tcgccttgcg cgtgataggc agcgccgaga tttcggtacc
    17941 caataaaacc gtccggagcc aactcgatgc tccggcggga agcggcgatg gcctcttcca
    18001 tccggcccat gttgtagtaa agggtgccga gcgcgtcgaa attcagcctg tggggctgga
    18061 cctccgccgc ctttttgtag gccgcttcgg cctctttgaa tcttccctgg gactcatgga
    18121 ttctcccagc ggcgtagtgg gcatcgccat tggccggctc gaggacgaga gcccgttcga
    18181 tatggcgcat ggcctcgtcg gtgcgcccgt cggacatgag cgccagcccc aggctgaccc
    18241 gcgcggcagc cagataggga tcgagtgcga cggcacgctc cgcgttggac aaggcccagg
    18301 tcagcaacat cgggtcccgg ctgtcgctcc gaaacttgaa ccagtaggct ctcgcaagcg
    18361 ccgcttgagc ggcggcatgg gtctggtctc gagcgagaac cttctggaag ctgtcgatcg
    18421 cctgatcgag attccccttg aggtcgggac gatcgaggta ggtcaacccc tcttggtaaa
    18481 ggctatgcgg cgttggctgg acagccggca agcgcagtga gtacgccgcg acgatcatga
    18541 cgaggattag aagggcgatc ccgatcgcgg tccgtaggct ccgaatcctc cgcagtggag
    18601 gttcctgacc cgctaagctt cccgaaagtt gggcgggctc agcggcggca gggagtccgt
    18661 ccggcaatgg ctggcccgca gtgcgctcga acgaggagga tgcctggatc tgccaggccg
    18721 gcatctcgag atccgtctcc tggccgcagg tttcagactc ttcaagggag acctgcgctc
    18781 ccgccgggaa aacctctcgt ttcgtggcga tgcgatccag atccgttgcc acctccgccg
    18841 cgctgcctgg ccggtaggcg ggatcttttt ccagaagccg atcgatcagg tcggaaagct
    18901 cgcgcggcac atcgggacgg atatccgacg ccgggcactg ccggaaactg cagacgcggg
    18961 cgagcgtggc ctgcgcggtt tcggcccgga acggcgcgac gcccgtgacc atctcgtaca
    19021 ggagagagcc gagggagaag agatccgatc gtgcgtcgag cggcagcccc atggcctgct
    19081 cgggcgacat cgcgtaggag gtccccacga tcgttcccgg cctcgacaag gtcgtctcc
//
LOCUS       DASPJI010000533        15201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_780117, whole genome shotgun sequence.
ACCESSION   DASPJI010000533 DASPJI010000000
VERSION     DASPJI010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15201)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15201
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780117"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1105)
                     /locus_tag="VEL74_14535"
     CDS             complement(158..1105)
                     /locus_tag="VEL74_14535"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HYG63794.1"
                     /translation="MRVTKSAFTVFALSALLLAGASVAQAQASRTWVSGTGDDVNPCN
                     RLAPCKTFAGAISKTAAGGQINVMDAGAFGAVTITKALTISAEGFFAGLTTSPGFNVI
                     VVNAGPNDVVVLRGLDIDLLNGGTNAIRFLAGGTLVVEDCILKNGTAKGIDFEPSGNS
                     QLLVKNTTIVGFNNGANGGAILIKPTGAGTAKVALTDVRMTRSTYGLRAENGSMVAMR
                     DSVASNNTNNGVIALSTAGGALDVSIDNSLISGNGIYGVRSEGSAAAIVRISNTTITG
                     NNPGLSAATNGQIISYGDNHNSGNVSNNNGAPTSTIARQ"
     gene            complement(1288..2220)
                     /locus_tag="VEL74_14540"
     CDS             complement(1288..2220)
                     /locus_tag="VEL74_14540"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HYG63795.1"
                     /translation="MRNKNLTLLALSALLFAGASRSEAQALRTWVSGIGNDNNPCSRT
                     APCKTFAGALLKTGPSGEINVLDPGGFGAVTITKAVTILADGATGGIQHSFINGITIN
                     AGPNDVVTLKGLTLESSGQGLNGIRFQAGGSLVVEDCIIRNGVQKGITFEPSGHSHLF
                     VRNTTIVNFNHGTNGGAILIKPGAAGSAKVVLDNVRMTRNLYGVRAENRSTVSVRDSV
                     ASDNTNNGFLAFSTGSPVDMSIESTLVSLNGINGIRSEGAAAVVRISNNTITGNNPGL
                     ATAGGGQIISYGDNNNSGNVGNNNGAPTSTIDRQ"
     gene            complement(2310..3233)
                     /locus_tag="VEL74_14545"
     CDS             complement(2310..3233)
                     /locus_tag="VEL74_14545"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HYG63796.1"
                     /translation="MAGFKLSIAMVSVSSARGYHLPMTSPRVRLLCPVRGCGEALDRV
                     DRSFRCPRGHSFDVARSGYLNLLQPQDRRSKQPGDPPEAVAARRRFHDAGHEEPLVRA
                     LLDILAPLPLPGRPAVPTVLDVGCGDGYHLGSLAAARDLEAHGLDLSVPAVDLAARRH
                     PGPLWVVANADRFLPYATGSFDFAMSLTARMNAPELRRVLSLEGRLLVAVAGADDLVE
                     LREAVLGEGRLRDRVERTVAEFAAAFELEEHRPLRYRLRLDTAGLADLLSLTYRGARE
                     SRRGRLAGLTAMEVTLSRDILVFKPRSLSPL"
     gene            3205..4572
                     /locus_tag="VEL74_14550"
     CDS             3205..4572
                     /locus_tag="VEL74_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HYG63797.1"
                     /translation="MAIESLNPATGETIRTYEEMTFQEVDGILERAHQAYLDWREAPF
                     SERARLMKGAGRVLRERKDEYARLMAEEMGKPLAAGRSEADKCAWVCDFYADHAERFL
                     SPETVATDASRSFVAFEPIGVVLAVMPWNFPFWQVFRFAAPGLMAGNAGVLKHASNVA
                     GCALAIEEVFRQAGFPADLFRTLLIGSRQVERVIEHRRVAAVTLTGSTPAGKAVAGKA
                     GACLKKTVLELGGSDPYLILEDADLEQAVETCVASRTINSGQSCIAAKRFIVPRSRAA
                     EVERLFVEKMRAKRLGDPLEEGSDLGPQAREDLRDGLHRQVEESIRRGARLLLGGEIP
                     ARPGAWYPPTVLTDVRPGMPAYDEETFGPVAAIIPVEDEEEAIRVANDTVFGLGAAVF
                     TRDIARGEEIATYRLEAGCCFVNDLVRSDPRLPFGGVKESGYGRELGPYGIKEFVNIK
                     TVYVK"
     gene            complement(4579..5346)
                     /locus_tag="VEL74_14555"
     CDS             complement(4579..5346)
                     /locus_tag="VEL74_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003934610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HYG63798.1"
                     /translation="MLANDIDDVLRILDGIIADCRTRRDPLGYFPALYRQVTRQVKRG
                     IAEGFFENGPRMIRFDALFANRYFMAYEAFRAGRKPTKSWQVAFGATCSGELIILQDL
                     LVGINAHVNLDLGVVAGEAFQGAALESFRGDFDKINLILGSLIPPVEQVVGRFSPLIH
                     ILDELGGKDEVEVLDFSLDAARDEAWLNATVVSLVPPGVRPFTIQSIDRKAAFLGRLI
                     ANPTGMVARAVSIIRATESRDVPAIIDALGAVVPPAR"
     gene            complement(5362..6000)
                     /locus_tag="VEL74_14560"
     CDS             complement(5362..6000)
                     /locus_tag="VEL74_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420238.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HYG63799.1"
                     /translation="MPTIVRPSAYDFIPDRPSLPKLWEAAQECTACELYKKGTQAVLG
                     EGPRHAAALFVGEQPGDQEDLAGKPFVGPAGRVLDEGLVTAGIPRDEVYVTNAVKHFK
                     WEPRGKRRIHQKPTLGEVKACRPWLETELALVKPKLIVCLGATAAQSLMGPEFRITRD
                     RGRFFETPWNALLTATLHPSAILRMPDAVKREEARVQFLEDLRKVAERLRAA"
     gene            complement(6127..6831)
                     /locus_tag="VEL74_14565"
     CDS             complement(6127..6831)
                     /locus_tag="VEL74_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010625998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYG63800.1"
                     /translation="MFEIQRKRAVAACLAPALALSLLAGCATTGTRDDAPRREPYVTK
                     RDRTLKGAGVGAAAGAVGAVLAGKREADEILAGAAIGAVVGGGVGAYMDRQQEKLARI
                     PGTTVERVGDDTLLLHFDSDVLFDVNSAVIDSSGRSTLEDVAGVLDEYDKTAVVIQGH
                     TDATGSEEHNQELSERRATSVRNYLVGRGVDDDRLSAVGFGEGYPVASNDSESGRQQN
                     RRVDILLKAKAGPVRG"
     gene            7077..7355
                     /locus_tag="VEL74_14570"
     CDS             7077..7355
                     /locus_tag="VEL74_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63801.1"
                     /translation="MIRPSLRRLSALALILVMTVLFAAPAGAGPVPREEPPAKEESGW
                     LDTALTVLSKLLPEKALDLTLPRLPLLPLDHTGSCIDPLGNPCIFRPW"
     gene            7611..7940
                     /locus_tag="VEL74_14575"
     CDS             7611..7940
                     /locus_tag="VEL74_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63802.1"
                     /translation="MMIRPFTRALSAAVVILALALLVASPVQAAPSKAPRLSAKLGSN
                     FLDAALSWLNGFLNDLAPAASERTEMAKVKPAPTGGGSGTPGGATVQTGSCIDPQGNP
                     CIFIPEP"
     gene            8003..9379
                     /locus_tag="VEL74_14580"
     CDS             8003..9379
                     /locus_tag="VEL74_14580"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG63803.1"
                     /translation="MLTSHPTAEDFNGFLSTATGQGAAARNALVLRHLLADCDTCREQ
                     LSAMGWDGRRLDRLLYLPGAEPLERQQVSYDYSAAFAKAERAYEALLDEVRPAEQAVE
                     KMWADLDSRTQEEQVRLVGSERRFQHPEMIRLLVERSHGLRYSDPAKMLHVADLARLA
                     SEAATPAVAGNELRLADLRTHSWRQYGNALRISGRITEAEEALTKAQKHCKEGTGDPP
                     LRAWLLEQMASLRIFQRRFEEAIEISDQAGQIYRDLGETHSLASTMVQKAIASLYSGE
                     PENAVRILNRAIPLIDNEEDPHLLLAACHNLVRCYIDLERPEQALSLYSEAREIYQEF
                     QDALIFLRAAWQEGQLLRDLGHLRAAETALVRARKGFMERSLAYEVAVVSLDLAAVYI
                     KLGEAESVRQTVAETVPIFRALRVDRDALASLLQLQQVATQGQQALELIRFLSSRLEK
                     LPSRQAGK"
     gene            9497..10828
                     /locus_tag="VEL74_14585"
     CDS             9497..10828
                     /locus_tag="VEL74_14585"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63804.1"
                     /translation="MLRDHPSEQDLELFLGGCAPKGGHIEVVRHLLAGCGECRRRLET
                     IGWSQDRLDRLIRFPRTKDLEKTDRQPAGASYNYERAFARAEKTLSAFLDRGRPVEQP
                     PQDLLAELFPFPPEEQTEPSDRRFAIPQLVRWLIERSHAIRYRDIEEMLHLARLARLA
                     VEACSVETVGSPQKLADLRIQAWGHFANSLRVCGRLEEAEQAFAVAEGHLPEGSGDPL
                     IRAFLLERMGSLHTSQRRFQRATELLEEAERLYQEMGVRWLRARSQILRGISLLYSGE
                     PEEASRLLRQAIPLIDPDEDPYLLLAARHNLVQCLIDLDRPHEALALYHEIRELNRQF
                     TDPLIQLRNTWQEGKLLRELGHMGPAEEKLQKARRGFMERGLAYEVAVVSLDLANVYA
                     KMGCTEEVNQLISETLPIFRALRIGRDMLAALIQLRQVARQERQALDQPPS"
     gene            complement(10850..11164)
                     /locus_tag="VEL74_14590"
     CDS             complement(10850..11164)
                     /locus_tag="VEL74_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63805.1"
                     /translation="MAALLRDLVVASGAPVEMIESRVGWEEGKLSALLEGRSGLSFTE
                     LLEVLQVMDTTPPEFFARLYGFEGNGPAQDPLDRMFEESRRVVRNAVNRRLAWKQDKG
                     QG"
     gene            11300..12235
                     /locus_tag="VEL74_14595"
     CDS             11300..12235
                     /locus_tag="VEL74_14595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HYG63806.1"
                     /translation="MPRLRIRVHPKSWPQLFKEIIASWNRHGAAAQSAALAFYTIFSL
                     APVLVVVIAVAGAVFGAEAVRGQIFGQFRGLMGDQAALLVQEVLKTAAQPAASRIATV
                     LGIGTLIFGATGVFAQLQDALNRVWDVEPQPGAVFTRLLRKRLLSFAIILVMGFLLMV
                     SLVLSAALAGFSAYLERILPIPVAVLEIANLLVSFGIITVLFAMIYRLLPDVKLEWHD
                     VWQGAIVTSLLFVIGKSLISFYLGRTGLASAYGAAGSLVLVLLWVYYSSLIFFFGAIF
                     TRVHSRQYRHRRAEPEEGAVRVPRGEASAVAQQNL"
     gene            12232..14052
                     /locus_tag="VEL74_14600"
     CDS             12232..14052
                     /locus_tag="VEL74_14600"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HYG63807.1"
                     /translation="MTTAIHRRLSAALLALLLLVPGPAADAEKVRIEVEGLERDLERN
                     VRAILSLDEARGDDDLTEDRIRRLHDRAQEEIELALQPFGYYRPTLQSTLERDGDTWV
                     ARYVVDPGPPLQVQTRDLRVEGEGADDPAIQEIVREFPLEQGETLYHPDYEEGKAAFD
                     DYAARNGYLDAAFDVSEIRVDLANYTSDVVLHYDTGPRYRFGPVLFHQNFLDPDLLRG
                     YVTFEEGEPLNVDEVLEMQNALSDSPYFERVEVVPRTEQREGLTVPIDVNLTPSKKQR
                     WTTGVGYGTDTGPRGTVGLELRRINRRGHRGRAEARISEIERSFQSSYEVPGPYPRTD
                     VLSYNVGYREEITDDIESESILVGASLSQARGDWREAFSLNLQREDFVVGELDRGRAE
                     LILPQASWSRVEADDRIFTTKGHRLQFEFRGTDDAIGSNVSLVQAEAEGKLIRSFSDN
                     FRFISRAQVGWTQTDQFRSLPASLRFFGGGDQSVRGYGYQELGRRVCFSVEGEELEGD
                     DCDGAAETFNIGGEALLTGSVEVEYQFLEEWRFLKKWGVAAFYDTGNAFESFSGDLES
                     GAGVGLRWLSPIGPIRADVAWALTEEGTPVRFHLTVGPDL"
     gene            14049..>15201
                     /locus_tag="VEL74_14605"
     CDS             14049..>15201
                     /locus_tag="VEL74_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63808.1"
                     /translation="MKNRRRLRKILLWSSVGLLTLVLLLGLSVWFLAGTQGGTEFLFT
                     RLGALMPGSLEVAELRGPLRGPLDIRGLKYEREGFSMNIERVQLEWRLRELADRQLDI
                     QRLYADGVRIVTTPSEEQKERSALPDLNLRFNIIVRDARVRDLSFASSAAEPGEKPFV
                     IDSIDLATTAMGGDVRVDSLAVRSPTFDADVNGKVRPQGDYPVDLNVRWALRLPDMAP
                     LSGAGKLTGTLEDLQVSQSLREPFDVQADLRLIEPLYELRFAGTISAPRFNPRLIKPD
                     LPDIPGEARITAEGTITNFTAKGNVRGTLPETGPVSADFQASRAGETGEQWLIQRADI
                     ALVGTPTRLTAQGTVTTGDTGEEVRFDGEASWRNLAWPLRGAQPMVVSRQ"
BASE COUNT         2400 a         5193 c         5110 g         2498 t
ORIGIN      
        1 catattctct ccttgatggt ctgccaccgc aaacctttat ggcgatctct cgatcgaatc
       61 ctggaaccta ggcgttgggg ggagtataac gttacatcgg ccggtgaagc tgaaacggga
      121 gaacggcccc cgccggccgg cgggggccag caggaaccta ctgccgcgcg atggtcgagg
      181 tcggggcgcc gttgttgttg ctgacgttgc ccgagttgtg gttgtccccg taggagatga
      241 tctgaccgtt cgtagcggcg gagaggcccg ggttgttgcc ggtgatcgtg gtgttgctga
      301 tccggacgat ggcggcggcc gacccttcgg agcggacgcc ataaatgccg ttgcccgaga
      361 tgaggctgtt gtcgatgctc acgtcgagcg cgccgccagc ggtcgagagg gcgatgacgc
      421 cattgttggt gttgttcgac gcgacgctgt cccgcatcgc caccatcgac ccgttctcgg
      481 cccgcagccc ataggtgctc cgcgtcatgc ggacgtccgt gagagccacc ttggccgtcc
      541 cggccccggt gggcttgatc aggatggcgc cgccgttggc gccgttgttg aagccgacga
      601 tggtggtgtt cttgacgagg agctggctgt tgccggaagg ctcgaagtcg atccccttcg
      661 cggtgccgtt cttgaggatg cagtcctcga cgaccagagt gccgccggcc aggaaccgga
      721 tggcattggt gcccccattg agcagatcga tgtccagccc gcgcaggacc accacgtcat
      781 tgggcccggc gttgacgaca atcacgttaa aaccgggaga ggtcgtgaga ccggcgaaga
      841 acccttcggc gctgatggtg agcgccttgg tgatcgtcac cgccccgaac gcgccggcgt
      901 ccatgacgtt gatctggccc cccgcggcgg tcttcgagat ggcccccgcg aatgtcttgc
      961 agggagccag gcggttgcat ggattgacgt cgtcgccggt gccggacacc caggtccggc
     1021 tcgcctgcgc ctgggcaacg gaggcgccgg cgagcagcag ggcggacaga gcgaagacgg
     1081 tgaacgcgga tttcgtgaca cgcatatctc tctcctttga gcccgctggg ggcccacgct
     1141 tggatctctg gaccagagct ggaaaccagt tgaacgctag ggatctgacg tgagagtgta
     1201 gccggttccc tcggggggga caaacgaggg agcgtatcgc aggagaagag ggcggccccc
     1261 gccgggcggc gggggccggg ccggggctca ctgccggtcg atggtcgagg tcggggcgcc
     1321 gttgttgttg ccgacgttgc ccgagttgtt gttgtcgccg taggagatga tctggccccc
     1381 gccggcggtg gccagcccgg ggttgttgcc ggtgatggtg ttgttgctga tccggacgac
     1441 ggccgcggca ccctcggagc ggatgccgtt gatgccgttg agcgacacca gggtgctctc
     1501 gatgctcatg tccaccgggc tgccggtcga gaaggccagg aagccgttgt tggtattgtc
     1561 cgacgccacg ctgtcacgga ccgagaccgt ggagcggttc tcggcccgca cgccatagag
     1621 gttccgcgtc atgcggacgt tgtcgaggac caccttggcg gacccggcgg cgccgggctt
     1681 gatcaagatg gcgccgccgt tggtgccgtg gttgaagttg acgatggtgg tgttccggac
     1741 gaagaggtgg ctgtggccgg acggctcgaa ggtgatccct ttctggaccc cgttccggat
     1801 gatgcagtcc tcgacgacga ggctgcctcc cgcctggaag cggatgccgt tcaagccctg
     1861 gccgctgctc tccagggtca accccttgag cgtgacgacg tcattgggcc cggcgttgat
     1921 ggtgatgccg ttgatgaagg agtgctggat tcccccagtg gctccgtcgg ccaggatagt
     1981 gacggccttg gtgatggtca cggcgccgaa gccacccgga tcgaggacgt tgatctcgcc
     2041 gctcgggccg gtcttgagga gggcgcccgc gaacgtcttg cagggggccg tacggctgca
     2101 cgggttgttg tcgttgccga tgcccgacac ccaggtccgg agcgcctgcg cctcgctcct
     2161 ggaggcgccg gcgaagagga gggccgacag ggcgagcagg gtcaggttct tgttccgcat
     2221 ggttctctcc ttgacagttt gccgttgcaa accctctgtc gatctctgga tccgatcgcc
     2281 caatccaggc gacggccagg gctcggacgt tacagggggc tcagcgagcg gggtttgaag
     2341 accaggatat cgcggctcag ggtgacctcc atcgccgtga ggccggcgag ccgcccgcgc
     2401 cggctctccc gggcgccccg gtaggtcagg gacaggagat ccgcgagccc ggccgtgtcg
     2461 aggcggagcc ggtagcggag aggacggtgc tcctccagct cgaaggccgc cgcgaactcg
     2521 gccaccgtcc gctccacccg gtcgcgcagc ctcccctccc ccagcaccgc ctcccgcagc
     2581 tccaccaggt cgtccgctcc ggccaccgcc accagcagcc ggccttcaag ggagaggaca
     2641 cgccggagct cgggggcgtt catccgcgcc gtgagggaca tcgcgaagtc gaaggacccg
     2701 gtcgcatagg ggaggaagcg gtcggcgttg gccaccaccc agagggggcc gggatggcgg
     2761 cgggccgcca ggtccacggc cgggacggag aggtcgagcc cgtgcgcctc caggtcgcgc
     2821 gcggcggcga gcgatcccag gtgatagccg tcgccgcagc cgacgtccag aacggtgggg
     2881 acggcggggc gtccggggag cggcaggggg gccaggatgt cgagcagtgc ccggaccagc
     2941 ggctcctcgt gcccggcgtc gtggaagcgg cgccgggcgg ccacggcctc gggcggatcg
     3001 cccggttgct tggagcgccg gtcctggggc tggaggaggt tgaggtagcc gctgcgggcg
     3061 acgtcaaagg agtgcccccg cgggcaccgg aaggagcggt ccaccctgtc cagggcttcc
     3121 ccgcagccgc ggaccgggca gagcaggcgg acccgcggcg aagtcatggg aagatgatag
     3181 ccgcgggcgg aggagaccga caccatggcg atcgagagct tgaaccctgc caccggcgag
     3241 acgatccgga cctacgagga gatgacgttc caggaggtgg acggcatcct ggaacgggcc
     3301 caccaagcct acctggactg gcgcgaggcc cccttcagcg aacgggcgcg cctgatgaag
     3361 ggcgccggcc gggtgctgcg ggagcgcaag gacgagtacg cccggctgat ggccgaggag
     3421 atgggcaagc ctctcgcggc gggccggtcc gaagccgaca aatgcgcctg ggtctgcgac
     3481 ttctacgcgg accatgccga gcgcttcctc tcccccgaga cggtggcgac cgacgcctcg
     3541 cgcagcttcg tggccttcga gccgatcggc gtggtcctcg cggtgatgcc ctggaacttc
     3601 cccttctggc aggtgttccg gttcgccgcg cccgggttga tggcgggcaa cgccggagtg
     3661 ctcaagcacg cctccaacgt cgccggctgc gcgctggcca tcgaggaggt cttccggcag
     3721 gccggcttcc ccgcggacct cttccggacc ctgctcatcg gcagccgcca ggtcgagcgg
     3781 gtcatcgagc accggcgggt ggccgccgtc accctgaccg gcagcacgcc ggcgggcaag
     3841 gcggtggccg gcaaggcggg ggcctgcctc aagaagaccg tgctggagct gggggggagc
     3901 gatccctacc tgatcctgga ggacgccgac ctcgagcagg cggtcgagac ctgcgtcgcc
     3961 agccgcacca tcaacagcgg ccagagctgc atcgcggcca agcggttcat cgtgccgcgc
     4021 tcccgggcgg ccgaggtgga gcgcctgttc gtcgagaaga tgcgggcgaa gaggctgggc
     4081 gatcccctgg aggagggctc cgacctcggc ccccaggcgc gggaggacct gcgcgacggc
     4141 ctgcaccggc aggtcgagga gagcatccgc cgcggggccc gcctccttct gggcggcgag
     4201 atccccgccc ggccgggggc gtggtatccg ccgacggtgc tgaccgacgt ccgcccgggc
     4261 atgccggcct acgacgagga gaccttcggg ccggtggccg cgatcatccc ggtggaggac
     4321 gaggaggagg cgatccgcgt cgctaacgac accgtcttcg gcttgggcgc ggcggtcttc
     4381 acccgagaca tcgcccgcgg cgaggagatc gccacctatc gcctcgaagc gggctgctgc
     4441 ttcgtcaacg acctcgtccg ctccgacccg cggctcccct tcggcggcgt caaggagtcg
     4501 ggctatggcc gcgagctcgg cccctacggc atcaaggagt tcgtcaacat caagacggtc
     4561 tacgtgaagt aggggccgtc accgcgccgg cggcaccacg gcgcccaggg cgtcgatgat
     4621 cgccggcacg tcccggctct cggtcgcgcg gatgatggag accgcccgcg ccaccattcc
     4681 ggtgggattg gcgatcagcc gtccaaggaa cgcggccttg cggtcgatcg actggatggt
     4741 gaacggccgc acgccgggcg gcaccaggga gacgacggtg gcgttgagcc aggcctcgtc
     4801 gcgggcggcg tccaggctaa agtcgaggac ctctacctcg tccttgccac cgagctcgtc
     4861 caggatgtgg atcagcgggg agaagcggcc gacgacctgc tccaccggcg ggatgaggct
     4921 ccccaggatc aggttgatct tgtcgaagtc gccgcggaac gactccaggg cagccccctg
     4981 gaacgcctcg ccggcgacga cgcccaggtc caggttgacg tgggcgttga tgcccaccag
     5041 caggtcctgg aggatgatga gctcccccga gcaggtggcg ccgaaggcga cctgccagct
     5101 cttggtcggc ttgcggccgg cgcggaaggc ctcgtaggcc atgaagtagc ggttggcgaa
     5161 gagggcgtcg aaccggatca tccgcggccc gttctcgaag aacccctccg cgatcccccg
     5221 cttcacctgg cgcgtcacct ggcgatacag ggccgggaag taccccagcg ggtcgcgccg
     5281 ggtccggcag tcggcgatga tgccgtccag gatccgcaac acgtcgtcaa tgtcgttggc
     5341 gagcataagg gaagtctacc ctcaggccgc ccgcagccgc tccgccacct ttctcagatc
     5401 ttccaggaac tggacccgcg cctcctcgcg cttcacggcg tccggcatgc gcaggatggc
     5461 cgaggggtgg agggtggcgg tgaggagggc gttccagggg gtctcgaaga agcggccgcg
     5521 gtcgcgggtg atccggaact cgggccccat gagcgactgg gcggcggtgg cgccgaggca
     5581 gacgatcagc ttcggcttga ccagggccaa ctcggtctcg agccacggcc ggcaggcctt
     5641 gacctccccc agcgtcggct tctggtggat gcgccgcttg ccccggggct cccacttgaa
     5701 gtgcttgacc gcgttggtga cgtagacctc gtcccgcggg atgcctgcgg tgaccaggcc
     5761 ctcgtccagt acccggccgg ccggtccgac gaagggcttg cccgccagat cctcctggtc
     5821 gcccggctgc tcgccgacga agagggcggc ggcatggcgc ggcccctccc cgaggacggc
     5881 ctgcgtacct ttcttataga gctcgcaggc ggtgcactcc tgggccgcct cccagagctt
     5941 aggtaggctc ggccggtcgg ggatgaagtc gtaggcggac ggtcgcacga tggtcggcat
     6001 gatctcgtct cccgtcatga cgggccatca agaaatatgc caaacgtcgc gctttggata
     6061 ccggaaccgg agaagagccc cgaagggata cctccggggc tcgtggggct gggcttcgga
     6121 aacagtctag ccgcggaccg ggccggcctt ggccttgagc aggatgtcga cacggcggtt
     6181 ctgctggcgt ccggactcgc tgtcgttgga ggccacgggg tagccctcgc cgaagcccac
     6241 cgcggacagc cggtcgtcgt cgacgccgcg gccgaccaga tagttgcgga ccgaggtcgc
     6301 ccgccgctcg gagagctcct ggttgtgctc ctcgcttccg gtggcgtcgg tgtgaccctg
     6361 gatgacgacg gcggtcttgt catactcgtc cagcaccccg gccacgtcct cgagagtgga
     6421 ccggccgctg gagtcgatca ccgccgaatt gacgtcgaag aggacgtcgg agtcgaaatg
     6481 gagcagcagg gtgtcgtcgc cgacccgctc gacggtggtg ccggggatgc gggcgagctt
     6541 ctcctgctgg cggtccatgt aggcgcccac gccgccgccg accacggcgc cgatggcggc
     6601 gccggccagg atctcgtccg cctcgcgctt gccggcgagg acggcgccga cggcaccggc
     6661 ggcggccccc acgcccgctc cctttagggt gcggtcgcgc ttggtcacgt acggctcgcg
     6721 ccgcggcgcg tcatcgcggg tgcccgtagt ggcgcagccc gccagaaggg ataatgcaag
     6781 ggccggggcc agacaggccg cgacggctcg ttttctctgg atctcgaaca tctcagtctc
     6841 ctttcccgcc ctctcccctc tccctcctcg caagcggcgg ttcaaccccc tggcttcaag
     6901 aggtatgcca gatcgcattc ccgccgctct cagccctcag gcgggccacg ctgacgcagc
     6961 tcgttgtcct ctacagagga cattgctgaa gccctcaaag ggcgtactat agaggagtag
     7021 ctatgcagcc ctcgtggcgt gcccggtatg aggagagcaa tcatcaagga ggatgtatga
     7081 tccgcccctc gctccgcagg ctatccgccc tcgccctgat cctggtcatg accgtgctct
     7141 tcgccgcccc agccggggcg gggccggttc cccgtgagga gccgccggcg aaggaagaat
     7201 ccggctggct ggacacggcc ctcaccgtcc tgagcaagct cctgcctgag aaggcactgg
     7261 acctcacact gccgcgactg ccactgcttc ccctggacca cacggggtcc tgcatcgacc
     7321 cgttgggcaa tccctgcatc ttccgtccct ggtagttgat cgcctgtcgc ggctgccctc
     7381 ctccgtcagg cccgccgggc ctgacggatc ttctgtcccg atcgtcacgg ggtgtcccgc
     7441 tttctcgtgg acaccacact tctacgtcag atccggttgt tattttttgg tagttggcag
     7501 ttctggtttg ttggcagctc tggtagttgg cagttctctt ttgcaggttt atacgcgggc
     7561 ggtttagcca taaaggctct acttgactcg cttctattga ggaggatgag atgatgatcc
     7621 gacccttcac ccgtgctctt tccgccgccg ttgtgatcct cgccctggct ctgctcgtgg
     7681 cctctccggt tcaggccgcc ccgagcaagg cgccgaggtt gtcggcgaag ttgggctcga
     7741 attttctgga tgcggcactc agttggttga acgggttcct gaatgacttg gccccggccg
     7801 cttccgagcg gaccgagatg gccaaggtaa agccggctcc tactggcggc ggcagcggca
     7861 cccctggcgg cgctacggtg cagaccgggt cgtgcatcga cccacagggg aacccctgta
     7921 tcttcattcc ggagccgtag aggtccgggg cgctgacgcc cctctgacct gagatggagt
     7981 tcagtccggg aggtagagag agatgttgac gagtcatccg actgccgagg atttcaacgg
     8041 cttcctgagc acggccaccg gacagggagc cgcagcgcgg aatgctctag tcctacgcca
     8101 tctgctggcc gactgcgaca cctgtcgcga gcagctctcg gccatggggt gggacggcag
     8161 gcgactcgat cgtcttctct accttcccgg ggctgagcca ctggagcgac agcaggtcag
     8221 ctatgactac agtgcggcgt tcgccaaggc agagcgcgcc tacgaggccc tcctggatga
     8281 ggtccgtccc gcagagcagg ccgtagaaaa gatgtgggcg gacttggact cccggacgca
     8341 agaggagcag gtgcggctcg tcggcagcga gcgacgcttc cagcatccag agatgatcag
     8401 gctcctcgtc gaacgcagcc acggcttgcg gtactcagat cccgccaaga tgctacacgt
     8461 cgccgatctg gcccgcctcg cctcggaggc cgcgacaccg gcagtcgcgg gcaacgagtt
     8521 gcgcctggct gatctgagga cccatagctg gcgccagtac ggcaacgcct tgcggatctc
     8581 aggccgcatc accgaggccg aggaagccct gacaaaggcc cagaagcact gcaaggaggg
     8641 caccggcgat ccgccgctcc gcgcctggct tctggagcag atggcctcgc tacggatctt
     8701 ccaacggcgt ttcgaggagg cgatcgagat ctcagaccaa gcggggcaga tctaccgtga
     8761 tttgggggag acccactccc tggccagcac catggttcag aaagcgatcg cgtcgctcta
     8821 ctcgggtgag ccggagaatg cggttcgcat cctgaatcgc gccatccctc tgatcgataa
     8881 cgaagaagac ccccacctgc tgctcgcggc ctgccacaac ctggtccggt gctatatcga
     8941 cctggagagg ccggagcagg cgctctcgct ctactccgag gcgagggaga tctatcaaga
     9001 attccaggac gcgctcatct tcctgcgcgc cgcttggcaa gaaggacagc tcctgcgcga
     9061 ccttggtcat cttcgcgccg ccgaaaccgc gctggttcgg gctcgcaagg gtttcatgga
     9121 aaggagcctc gcctacgagg tggcggtggt ctccctcgat ttggccgccg tctacatcaa
     9181 gttgggggaa gcggagagtg tcaggcagac cgtcgcggag acggtcccca tcttccgcgc
     9241 ccttcgtgtc gaccgggatg ctctggcctc ccttctccaa cttcagcagg tcgcaaccca
     9301 aggacagcag gcgctcgagc tcatccgctt cttgagttcc cggctggaga agctgcccag
     9361 ccggcaagca ggcaaataag ccgatccacc ggcggcggag gagaccgccg ccggtgccct
     9421 caccctgcac ccgattcgtt cttccgctaa aatagtttat cgggtggggc cgtgacggtc
     9481 cgggcggaag aagatcgtgc ttcgagatca tcccagcgag caggacttgg agctcttcct
     9541 tggcggctgc gcgcccaagg gcggccacat cgaggtggtg aggcacctgc tggccggctg
     9601 cggggagtgc cggagacgat tggagacgat cggctggagc caggaccgtc tggatcgtct
     9661 gatccggttt ccccggacaa aggatctgga gaagacggac aggcagccgg caggcgcctc
     9721 ctacaactat gaacgagcct tcgccagggc cgagaagacg ttgtcggcct ttctggaccg
     9781 cgggcgtccg gtcgagcagc ctccccagga cctcctcgcg gagctctttc cctttccacc
     9841 cgaggaacag acggagccca gcgacaggcg cttcgccatt cctcagctcg tccgctggct
     9901 catcgagcgc agccatgcca tccggtaccg ggatatcgaa gagatgctcc atctcgcccg
     9961 tctggctcgc ctggccgtcg aagcctgttc cgtcgaaacc gtaggcagtc cccagaagct
    10021 ggcggatctc cgaatccagg cttggggtca cttcgccaat tcgttgcgcg tctgcggacg
    10081 actggaagag gccgagcaag ccttcgccgt cgccgagggc caccttccgg aggggagcgg
    10141 tgatcccctg atccgggcgt ttctcctcga acgcatggga tccctccata ccagccagcg
    10201 gcgcttccag cgggcgaccg agctgctcga ggaagcagag cgcctgtacc aggagatggg
    10261 ggttcgctgg ctccgcgccc gctcacagat cctcaggggc atctcccttc tctattccgg
    10321 agaaccggaa gaggcttccc ggctcctccg gcaggccatc cccctgatcg acccggacga
    10381 ggatccctac ctcctcctcg cggccaggca caacctggtc cagtgcctca tcgacctgga
    10441 ccggccgcat gaggccctgg ctctctacca tgagatccgg gagctcaacc ggcagttcac
    10501 cgatcccctg atccagctcc gcaatacctg gcaggaggga aagctcctcc gcgagctcgg
    10561 gcacatggga cctgcagaag agaagctgca gaaggcccgc cggggcttca tggagcgagg
    10621 gctggcgtat gaggtggccg tggtctccct cgatctggcc aacgtctacg ccaagatggg
    10681 ctgcaccgaa gaggtcaacc agctcatctc cgagacgctc cccatcttcc gcgccctgcg
    10741 gatcgggcgc gacatgctcg ccgccctgat ccagctccgc caggtcgccc gccaggagcg
    10801 gcaggccctc gaccagcccc cctcctgaat cctgggcggc cttcccccct tagccttgcc
    10861 ccttgtcctg cttccaggcg agccgacggt tgaccgcgtt gcggaccacg cggcggctct
    10921 cttcgaacat ccggtccagg ggatcctggg ccggaccgtt gccctcgaaa ccataaagcc
    10981 gcgcaaagaa ctccggcggc gtcgtgtcca tcacctgcaa gacctccagt agctcggtga
    11041 agctgagccc gcttcgtcct tccagaagag ccgacaactt cccctcctcc cagcccaccc
    11101 ggctctcgat catctccacc ggtgcgccac ttgcaacgac caaatcgcgg agcaaggcgg
    11161 ccacccgcct ttcctcttga tgtacgggca tggctggaga tcctagcatc gacagccttc
    11221 gcttcgaagg gaaagcttcg tggcacgcct gttgcttgtt gccaggagcc tccggacgtg
    11281 tctccggtga gcagcctgta tgccgagatt gcgtatccgc gtccatccca agagctggcc
    11341 ccagctattc aaggagatca tcgcgagctg gaaccggcac ggtgccgccg cccagagcgc
    11401 ggcgctcgcc ttctacacca tcttctcgct cgcccccgtg ctggtggtgg tgatcgccgt
    11461 ggccggcgcc gtgttcggcg cggaggcggt gcgggggcag atcttcggcc agttccgcgg
    11521 cctcatgggg gaccaggcgg ccctgctcgt ccaggaggtg ctcaagaccg ccgcccaacc
    11581 ggccgccagc cggatcgcca ccgtgctcgg catcggcacc ctgatcttcg gcgccaccgg
    11641 cgtcttcgcc cagctccagg acgcgctcaa ccgggtatgg gacgtggagc cccagccggg
    11701 cgcggtgttc acccggctgc tgcgcaaacg cctgctctcc ttcgccatca tcctggtgat
    11761 gggcttcctg ctgatggtct cgctcgtgct gagcgccgcc ctcgccggct tcagcgccta
    11821 cctggagcgg atactgccca tccccgtcgc ggtcctggag atcgcgaacc tcctggtgtc
    11881 gttcggcatc atcacggtgc tgttcgccat gatctatcgc ctgctgccgg acgtgaagct
    11941 cgaatggcac gacgtctggc aaggcgcgat cgtgacctcg ctcctgttcg tcatcggcaa
    12001 gtcgctgatc agcttctatc tcggccgcac cggtctcgcc tccgcctatg gcgcggcggg
    12061 ctccctggtg ctggtcctgc tctgggtcta ctactcctct ctcatcttct tcttcggcgc
    12121 catcttcacg cgcgtccact cccggcaata ccggcaccgc cgtgccgagc ccgaggaggg
    12181 tgcggtgcgc gtcccccggg gcgaggcgag cgccgtggct caacaaaacc tatgaccact
    12241 gccatccatc gccgactgtc cgccgccctc ctcgccctcc tgctgctcgt tcccgggcct
    12301 gccgccgacg cggagaaggt ccgcatcgag gtggaggggc tggagcgcga cctcgaacgc
    12361 aacgtccgcg ccatcctgag cctcgacgag gcgcgggggg acgacgacct caccgaggac
    12421 cgcatccgcc gcctccacga ccgcgcccag gaggagatcg agctcgccct ccagcccttc
    12481 ggctattacc ggccgaccct ccagagcacc ctcgagagag acggcgatac ttgggtggca
    12541 cgctacgtcg tcgaccccgg gccgcccctc caggtgcaga cgcgggacct tcgggtcgag
    12601 ggcgaggggg cggacgaccc cgccatccag gagatcgtcc gcgagttccc gctcgagcag
    12661 ggagaaacgc tctaccaccc cgactacgag gagggcaagg cggccttcga cgactacgcc
    12721 gcccgcaacg gctacctgga cgccgccttc gacgtcagcg agatccgggt cgacctcgcc
    12781 aactacacgt cggacgtcgt cctccattac gacaccgggc cgcgctaccg gttcggcccc
    12841 gtcctcttcc accagaactt cctcgacccg gacctgctgc gcggctacgt caccttcgag
    12901 gagggggagc cgctcaacgt cgacgaggtg ctggagatgc agaacgcgct ctccgacagc
    12961 ccctacttcg agcgcgtcga ggtggtcccc cggaccgagc agcgcgaggg actcaccgtc
    13021 cccatcgacg tcaacctgac ccccagcaag aagcagcgct ggacgaccgg cgtcggctac
    13081 ggcaccgaca ccggaccgcg cggcacggtg ggcctcgagc tgcggcggat caaccggcgc
    13141 ggccaccgcg ggcgcgccga ggcgcgcatc tcggagatcg agcgcagctt ccagtcgagc
    13201 tacgaggtcc ccgggccgta cccccgcacc gacgtgctga gctacaacgt cggctaccgc
    13261 gaggagatca ccgacgacat cgagagcgag agcatcctgg tgggtgcgag cctcagccag
    13321 gcgcgcggcg actggcgcga ggccttctcg ctcaacctcc agcgcgagga cttcgtcgtc
    13381 ggcgagctcg accgcggccg ggcggagctg atcctccccc aggcgagttg gagccgggtc
    13441 gaggccgacg accgcatctt caccaccaag gggcaccggc tgcagttcga attccgcggc
    13501 accgacgacg cgatcggctc gaacgtctcg ctcgtgcagg ccgaggcgga gggcaagctg
    13561 atccgctctt tctcggacaa tttccggttc atcagccgcg cccaggtagg ctggacccag
    13621 accgaccagt tccgcagcct ccccgccagc cttcgcttct tcggcggcgg cgaccagagc
    13681 gtccgcggct acggctacca ggagctcggc cgccgcgtct gcttctccgt cgagggggag
    13741 gaactggagg gggacgactg cgacggcgcc gccgagacct tcaacatcgg cggcgaggcc
    13801 ctgctcaccg gcagcgtcga ggtcgagtac cagttcctgg aggagtggcg gttcctcaag
    13861 aagtggggtg tcgccgcctt ctatgacacc ggcaacgcct tcgaatcctt ctccggcgac
    13921 ctcgagagcg gcgccggcgt cggcctgcgc tggctctccc ccatcggacc gatccgcgcc
    13981 gacgtcgcct gggcgctcac cgaggaaggc acacccgtgc gcttccatct caccgtagga
    14041 cccgacctgt gaagaatcga cgccgtctcc gcaagatcct tctctggagc tccgtggggc
    14101 tcctcaccct cgtcctcctg ctcggcctga gcgtctggtt cctggccggc acccaggggg
    14161 gcaccgagtt cctcttcacc cgtctcggcg cgctgatgcc cggctcgctc gaggtcgccg
    14221 agctcagagg ccccctgcgc ggcccgctcg acatccgcgg cctcaagtac gagcgcgagg
    14281 ggttctcgat gaacatcgag cgcgtgcagc tcgaatggcg cctgcgcgag ctcgccgacc
    14341 gccagctcga catccagcgc ctgtacgccg acggcgtccg catcgtcacc acgccgtcgg
    14401 aggagcagaa ggagcgctcg gcgctgccgg acctcaacct gcgcttcaac atcatcgtcc
    14461 gcgacgcgcg ggtgcgggac ctgagcttcg cctcctcggc cgccgagccg ggcgagaagc
    14521 cgttcgtcat cgacagcatc gacctcgcga ccacggccat gggcggcgac gtccgggtcg
    14581 acagcctcgc cgtccgctcc cccaccttcg acgccgacgt caacggcaag gtgcggccgc
    14641 agggggacta ccccgtcgac ctcaacgtcc gctgggcgct ccgcctgccg gacatggcgc
    14701 cgctctccgg cgccggcaag ctcacgggga cgctggaaga cctccaggtg tcgcagagcc
    14761 tgcgcgagcc gttcgacgtg caggccgacc tgcggctgat cgaaccgctc tacgagctgc
    14821 gcttcgccgg cacgatcagc gcgccgcggt tcaacccgcg cctcatcaag cccgacctgc
    14881 cggacatccc gggggaggcc cggatcaccg ccgaggggac gatcaccaac ttcaccgcca
    14941 agggcaacgt gcgcggcacg ctgccggaga ccgggccggt gtccgccgac ttccaggcca
    15001 gccgcgccgg cgagaccggc gagcagtggc tgatccagcg cgccgatatc gccctggtcg
    15061 gcaccccgac gcggctgacc gcccagggga cggtcactac tggtgacacc ggcgaggagg
    15121 tgcgcttcga cggcgaggcg agctggcgca acctcgcctg gccgctgcgc ggcgcccagc
    15181 ccatggtggt gagccgccag g
//
LOCUS       DASPJI010000534         4800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_33923, whole genome shotgun sequence.
ACCESSION   DASPJI010000534 DASPJI010000000
VERSION     DASPJI010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33923"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VEL74_14610"
     CDS             complement(<1..1179)
                     /locus_tag="VEL74_14610"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYG63809.1"
                     /translation="MATVTFLGACGTVTGSSTLLTWGDRGVLIDCGLYQGGEELEQRN
                     WSPFPFQPRKLEAVLVTHAHLDHTGLLPRLAAQGFMGTIYCTRPSRGLISLLLEDSAE
                     IQEEEVRYARGKGYSRHPRPEPLYTVEDAKRVLKLLRPVAMDDEHELFPGVRFRFRRA
                     GHLLGAASVEVTGKGADGERRTWCFSGDVGRYGVPILKDPEPPLAAPSALLLESTYGD
                     RHHPADDPADALASIIEETFERGGTVVIPAFALGRTQELLYHLAALTDRGRLDPGVVF
                     LDSPMAINATELYSRAQSEHDEEMAALVEESGGPLDPGRFQRCRTVTQSKALNNRREP
                     AVIVAASGMATGGRVVHHLLHRVSDPRNAVVLAGFQAAGTRGRALVDGADTIAIHGMP
                     V"
     gene            1295..1765
                     /locus_tag="VEL74_14615"
     CDS             1295..1765
                     /locus_tag="VEL74_14615"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63810.1"
                     /translation="MADTVDPRGPLEGAETARTYGNDTADDFGDAAADGDSDGETVVE
                     AQDRFQGSQSRYQRVSEDVRRGAERARAEFQRGSERAREEFQRGSVRAREGYRHVAES
                     ARVGYDRVRTDAGRLGGEVSVFVREKPAQAVLIAAGVGFLLGMLMRRRDDDYEE"
     gene            1834..2052
                     /locus_tag="VEL74_14620"
     CDS             1834..2052
                     /locus_tag="VEL74_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63811.1"
                     /translation="MNDSFLDEILDELLPEELDWADLVRRYPVPAVLLAAVGGFFLGR
                     SHGPSILTAASSYAASEMAKNVNELLGQ"
     gene            2071..2988
                     /locus_tag="VEL74_14625"
     CDS             2071..2988
                     /locus_tag="VEL74_14625"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HYG63812.1"
                     /translation="MRTLFHISDVHFGPPHLPRVSQGVLDFIERHRPDVVVLSGDLTQ
                     RARPEQFRQAREFVDRIPVPTVVVPGNHDVPMYRVWERVFSPFGAYRKYFSEELEPTY
                     RDDEMLIVGINTAFNWTIKDGRIKLRRLLEVAEILQKTPETVFKVVVAHHHMIPPPNF
                     GTQRVLGNAYEAIDLFSSAGVDLILSGHLHQAYIGNSEEFYPKGRPPVVILHSGTTTS
                     NRGRGGERERNTCNWIRVDGDSMVVSHFRWHHSLERFAEHSRHWYPRQERTPYTLEGL
                     SVEGIVPTPRGEVAAAVQPVPQPDLQPRQ"
     gene            3061..3357
                     /locus_tag="VEL74_14630"
     CDS             3061..3357
                     /locus_tag="VEL74_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63813.1"
                     /translation="MAEEWEIVKIVGTDAEAAVLVGFLNSSDIPAEAESLHASEFPTD
                     LGRLGEVRVRVPAERAQEALALLNSREDVATGEDGAMAGAPLAGTPEGEEPIRS"
     gene            3354..>4800
                     /locus_tag="VEL74_14635"
     CDS             3354..>4800
                     /locus_tag="VEL74_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYG63814.1"
                     /translation="MSSLASGQSVGRYRVVRFLGSGAMGEVYLAEDPHIERKLAIKTV
                     RLAGRPQEIEDRKKRLLREARAAGRLLHPYVVTLFDAGEVEGLLYLAFEFVEGDDLGS
                     RLAGGWRPSLGEVLRIVRQTAEALDYAHGQGIVHRDIKPSNILLDPAGRVKVADFGIA
                     KMADQSTELTVAGAVMGSPQYLSPEQIRGDDLDGRSDVFSLGVLFYELLSGRRPFEGD
                     TITTLVYQILHKEPVAVTGMRPLPPRLEALLRRMLQKDRDQRVASASQVAEEIAVLQS
                     ELPAEALAAPAADTGQMDETHVLPRRTTAPQGRVVPPPPPPPQSPPTLSAPSPRANPA
                     PPPPPAAGTAVAQPARPGGARRWIALAAVLLLVAMVAGGAVAWKVMFSGEPGTDEQVA
                     AVPTARMDGQPGQDPASATLPGTPTPAPVASPTPPPAMQQVEPSPSLAFRREPQGVPQ
                     GTPQGTMVGREPLPRPQRDRVDVPAPEVRNPG"
BASE COUNT          699 a         1709 c         1719 g          673 t
ORIGIN      
        1 caccggcatg ccatggatgg cgatagtgtc ggcgccgtcc accagcgccc gcccccgggt
       61 gcccgccgcc tggaagccgg cgagcaccac ggcgttgcgc ggatcgctca cccggtgcag
      121 gaggtggtgt accacccggc cgcccgtggc catgccgctt gcggccacga tcaccgccgg
      181 ctcccggcgg ttgttcaggg ccttggactg cgtcaccgtg cggcagcgct ggaaacggcc
      241 cgggtcgagc ggaccgcccg actcctcgac cagcgccgcc atctcctcgt cgtgctcgct
      301 ctgggcccgg ctgtagagct ccgtggcgtt gatggccatg gggctgtcca ggaagaccac
      361 cccgggatcg agccggccgc ggtcggtgag ggcggcgagg tgatagagca gctcctgcgt
      421 ccgtcccagg gcgaaggcgg ggatgaccac cgtgccgccg cgctcgaacg tctcctcgat
      481 gatggaggcc aacgcgtccg ccggatcgtc cgcggggtgg tgccggtcgc cgtaggtgga
      541 ctcgaggagc agagccgagg gggccgcgag cgggggctcg gggtccttga ggatcggcac
      601 gccgtagcgg ccgacatcgc ccgagaagca ccaggtccgc cgctcgccgt ccgccccctt
      661 gcccgtgacc tcgacgctgg ccgcgcccag caggtggccg gcgcggcgga accggaagcg
      721 cacgccgggg aacagctcgt gctcgtcgtc catggcgacg gggcgcagga gcttgagcac
      781 ccgcttcgcg tcctccacgg tgtagagcgg ctccggccgg ggatggcggc tgtacccctt
      841 gccgcgggcg tagcggacct cttcttcctg gatctcggcg gaatcctcga ggaggaggga
      901 gatcaagccg cggctcgggc gcgtacagta gatcgtcccc atgaagccct gggccgccag
      961 gcgggggagg agccccgtgt ggtcgaggtg ggcgtgggtg acgaggaccg cctccagctt
     1021 gcgcggctgg aaggggaagg ggctccagtt gcgctgctcg agctcctcgc ccccctggta
     1081 gaggccgcaa tcgatgagaa cgccgcgatc cccccaggtg aggagcgtgg agctgccggt
     1141 gacggtgccg caggcgccga ggaaggtgac ggtggccatg gcggagacga tatcccagag
     1201 gcacgattcc tgctagaaat ggcagaatgc tcgcgtcagc cggcccttcg ggccggctcg
     1261 agtccaacct cttccgagat acggaggttt ttccatggct gacacggtcg atcccagagg
     1321 gccgttggag ggtgccgaaa cggcacgtac gtatggcaat gacaccgccg acgatttcgg
     1381 cgacgccgca gccgacggcg acagcgacgg tgagaccgtg gtggaggccc aggatcgttt
     1441 ccagggctcc cagagccggt accagcgggt ctccgaggat gtccgccgcg gcgccgagcg
     1501 ggcccgcgcg gagttccagc gcggttccga gcgggcgcgg gaggagttcc agcgcggcag
     1561 cgtgcgggcg cgcgagggct accgtcacgt cgccgagagc gcccgcgtcg gctacgaccg
     1621 ggtgcgcacc gacgccggcc ggctcggcgg cgaggtcagc gtcttcgtgc gtgagaagcc
     1681 ggcccaggcc gtgctgatcg ccgccggcgt cggcttcctg ctcggcatgc tgatgcggcg
     1741 gcgcgacgac gactacgagg aatagtggac cccgccacgg gcgccgcggt ggcgcggcgc
     1801 ccgatggccc gttttgccac acgggaggac gcgatgaacg attcctttct cgacgagatc
     1861 ctggacgagc tgctgccgga ggagctggac tgggcggacc tggtgcggcg ctatccggtc
     1921 ccggcggtgc tgctggccgc ggtgggcggc ttcttcctcg gacgcagcca cggcccctcc
     1981 atcctgaccg ccgcctccag ctacgcggcg tccgagatgg ccaagaacgt caacgagctc
     2041 ctggggcaat agggcggccg cctccgaccc gtgcgcaccc tcttccatat ctcggacgtc
     2101 cacttcgggc ctccccatct gccccgagtc tcccaggggg tgctcgactt catcgagcgc
     2161 caccggccgg acgtggtggt gctctccggc gacctcaccc agcgcgcccg gcccgagcag
     2221 ttccgccagg cgcgggagtt cgtcgaccgc attccggtgc ccacggtggt ggtgccgggc
     2281 aaccacgacg tccccatgta ccgggtatgg gagcgggtct tctccccctt cggcgcctac
     2341 cggaagtatt tcagcgagga gctcgagccc acctaccggg acgacgagat gctcatcgtc
     2401 gggatcaaca cggcgttcaa ctggacgatc aaggacggcc gcatcaagct ccggcggctg
     2461 ctcgaagtcg cggagatcct ccagaagacg cccgagaccg ttttcaaggt ggtggtggcc
     2521 caccatcaca tgatcccgcc ccccaacttc ggcacccagc gggtcctcgg caacgcctac
     2581 gaggcgatcg acctgttctc gagcgccggc gtcgacctga tcctctcggg ccatctgcac
     2641 caggcctaca tcggcaactc cgaggagttc tatccgaagg gaaggccgcc ggtggtcatc
     2701 ctgcactccg ggacgacgac gtcgaaccgc ggccggggag gcgagcggga gaggaacacc
     2761 tgcaactgga tccgggtgga cggcgactcc atggtggtct cccacttccg ttggcaccac
     2821 agcctggagc gcttcgccga gcacagccgg cactggtatc cgcgccagga gcggacccct
     2881 tataccctgg aggggctatc ggtcgaaggg atcgtgccga cgccgcgcgg cgaggtcgcg
     2941 gccgcggtcc agcccgtccc gcagcccgac ctccagcccc gccagtagag tgcgaccgcg
     3001 ccggccggag atcccggacc ggctaagata tgatgtccct tcaacaggtg gaggctttgc
     3061 atggccgaag agtgggaaat cgtcaagatc gtgggtaccg acgccgaggc cgccgtcctg
     3121 gtgggcttcc tgaacagcag cgacatccct gccgaggccg agtccctgca cgccagtgag
     3181 ttcccgaccg acctcggccg tctgggcgag gtgcgggtgc gggtcccggc cgagcgcgcc
     3241 caggaggcgc tggcgctgct caactcccgc gaggacgtgg ccacgggtga ggacggggcg
     3301 atggcgggcg ctcctctggc cggcaccccc gaaggcgagg aaccgatccg ctcgtgagct
     3361 ccctggcgag cggccagagt gtcggccgct accgcgtcgt ccgcttcctg ggatcggggg
     3421 caatgggtga ggtctacctc gccgaggatc cccacatcga aaggaagctg gccatcaaga
     3481 cggtccggct ggccggccgt ccccaggaga tcgaggaccg caagaagcgc ctgctgcgcg
     3541 aggcccgcgc cgcaggccgc ctgctccatc cctatgtggt gacgctgttc gacgccggcg
     3601 aggtggaggg tctcctctac ctcgccttcg aattcgtcga gggggacgac ctggggagcc
     3661 ggctcgccgg cggctggcgg cccagcctgg gcgaggtgct gcgcatcgtc cgccagaccg
     3721 ccgaggccct ggactacgcc catggccagg ggatcgtcca ccgcgacatc aagccgagca
     3781 acatcctgct cgaccccgcg ggccgggtga aggtcgccga cttcggcatc gccaagatgg
     3841 ccgaccagag caccgagctc accgtcgcgg gcgcggtcat gggcagcccc cagtacctgt
     3901 cgcccgagca gatccgcggc gacgacctcg acggccgcag cgacgtcttc tccctggggg
     3961 tgctcttcta cgagctgctc tcggggcgcc ggcctttcga gggcgacacc atcacgaccc
     4021 tggtctatca gatcctgcac aaggagccgg tggcggtcac cggcatgcgc ccgctgccgc
     4081 cgcgcctcga ggctctgttg cggcgcatgc tgcagaagga ccgcgaccag cgcgtcgcgt
     4141 cggcctcgca ggtggcggag gagatcgccg ttctccagag cgagttgccg gcggaggccc
     4201 ttgccgctcc ggcggcggac accgggcaga tggacgagac ccatgtgctg ccgcggcgca
     4261 ccaccgcccc ccagggccgg gtggtaccgc ctccgccgcc cccaccccaa tccccgccga
     4321 cgctctccgc cccatctccc agggcgaacc cggcgcctcc gccgcccccg gcggccggca
     4381 cggcggtcgc ccagccggcg cggccggggg gagcgcgccg ctggatcgcc ctcgccgccg
     4441 tgctgctcct ggtggcgatg gtcgccggag gggccgtggc ctggaaggtg atgttcagcg
     4501 gcgagcccgg gacggacgag caggtcgccg cggtccccac ggcccggatg gacggccagc
     4561 cggggcagga cccggcgtcg gcgacgcttc cggggacgcc cacgccggct ccggtggcct
     4621 ctcccacccc gccgcccgcc atgcagcagg tggagccctc gccctcgctg gccttccgtc
     4681 gggagcccca gggagtgcct cagggcacgc cccaagggac gatggtgggc cgcgagccgc
     4741 tcccgcgccc gcagcgcgac cgggtcgacg ttcccgcgcc ggaggtccgg aaccccggcc
//
LOCUS       DASPJI010000535         3682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_331257, whole genome shotgun sequence.
ACCESSION   DASPJI010000535 DASPJI010000000
VERSION     DASPJI010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331257"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VEL74_14640"
     CDS             complement(<1..564)
                     /locus_tag="VEL74_14640"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HYG63815.1"
                     /translation="MSSIPVPCYDALVIGGGPAGATAALSMVRAGLSVRLVERTPHPR
                     FHVGESFLPRNLTLLRDLGLEPALQSLTHVAKHGAEFLAGDGSTGYLFSFQEALLNPE
                     PAAFNIERAPFDAMILDAARAAGTEVTEGVAVREIPVLEDGRVEAVLDTGERIAARYL
                     VACCGGRSTIPGALDVRWEGTMVLSHNL"
     gene            899..>3682
                     /locus_tag="VEL74_14645"
     CDS             899..>3682
                     /locus_tag="VEL74_14645"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG63816.1"
                     /translation="MKAEEASRRASDLSPEKLALLVMRMKKKSAEAPQAVKIPRRASA
                     EPPPLSFAQQRLWLLDHLEPGTSAYNMPFPARFTGALDVRVLRAAFDEIVRRHESLRT
                     TFALRETPEKGREAAPVQVISPPAPFLLPLIDLSLVPEPARGQESQRLLSAEAKTPFD
                     LARGPLLRAALVRLSPRDHLLLVDMHHIISDGWSCGLFFKELSDLYAAFAEKRSSPLP
                     ELPIQYSDFAVWQRDWLRGEVLEEQLRYWREQLAGSPPMLDLPTDRLRPAVQTHNGGS
                     VIHRFPADLTRALRETSRREGASLFMILLAGFKLILSRLAAQDEVVVGAPSAGRKRVE
                     TEGLIGFFLNTLVLRTHLAGDPSFRELLGRVKRVVLGAYRFQDVPFEKLLEELQPERH
                     LSRSPLFQVLFNMITVPEARLDLPGLHIDMLPAPEPESKFDFTLYVQEQPDALQIDLV
                     YNADLFDRARMEEMLRQFEHVMAQSAADPERRIGDYSLVSPVAAALLPDPAATLGGEW
                     MGAVHHALSRHAGEDAEAPAVTDWRGERWSRGELEARSNQLAWYLQASGVRTGDVVAV
                     WAHRSASLVWAVMGTLKAGAAFMILDPAYPTARLLDYLEIGRPAGWLAVEGAAAPPAE
                     VEEALRTAGCRCRLELPPLSAAMEKGLLANFPPFDPGVPVTADDAAVITFTSGSTGKP
                     KGVVGRHGPLSHFYPWMARRFELSEAERFGMLSALSHDPLQRDLFTPVWLGASIHVPD
                     PEQISTPGYLSDWARSEGVTMLHLTPAMMEILLGSAEERGEELPSLRRALVVGDLLKR
                     SDVARLQRLCPNLMAVNLYGSTETQRSVACFEVPRPEVWPAGPLDKLVLPLGRGMEDV
                     QLLILNRAQKLCGVGEVGEIYMRSRHLARGYLGDEALTTSRFLPNPFAPLAPEADGDR
                     VYKTGDLGRYLPDG"
BASE COUNT          506 a         1341 c         1275 g          560 t
ORIGIN      
        1 gaggttatgg gacaggacca tcgtcccctc ccagcgcacg tcgagcgccc ccggaatggt
       61 gctgcggccg ccgcagcaag cgaccagata gcgggccgcg atccgctcgc cggtgtcgag
      121 gacggcctcc acccggccgt cttccagcac cgggatctcg cgcaccgcca cgccctcggt
      181 gacctccgtc cccgctgccc gggcggcgtc caggatcatg gcgtcgaagg gggcgcgctc
      241 gatgttgaag gccgccggct cggggttgag gagcgcctcc tggaagctga agagatagcc
      301 ggtgctgccg tcgcccgcca ggaactccgc accgtgcttg gcgacgtggg tcagcgactg
      361 gagggcgggc tccagcccca ggtcccggag cagggtcagg ttgcgcggca ggaacgactc
      421 gccgacatgg aagcgcgggt ggggcgtgcg ctccaccaga cggaccgaga ggccggcccg
      481 caccatggac agcgccgcgg tggccccggc gggaccgccg ccgatcacca gggcgtcgta
      541 gcagggaacg ggaatggaag acacgacaag acctcggaaa aaaaacggct agcgcaggta
      601 tccgatagag agcttatcgg ccggcaggcg gggcgtcaaa tcggccgtgt gactgtcggc
      661 tcaaattcga cttcttcaat attcgtcctc gttgtccccg tttggccctc cagccacact
      721 taggcaacat cagggccgat ccagggtgac gggggcaggg gggccggtgc gatgccggtt
      781 gcttccgacg ggtctggccc aagcggtcgg gagaccgctt cttggtactt ctatctattt
      841 gatgtctgct gacttgtttt gagccagcgg ctgctgccgc tgcgtccgag ggagggttat
      901 gaaggccgag gaagcatcca ggcgagcgtc cgacctgtcg cccgagaagc tggcgcttct
      961 cgtgatgcgg atgaagaaga agtcggcgga ggcgccgcag gcggtgaaga tcccccgccg
     1021 ggcctcggcc gagcccccac cgctctcatt cgcccagcag cgcctctggc tcctcgatca
     1081 cctggagccc ggcacctccg cctacaacat gccgtttccg gcgcgcttca ccggcgcgct
     1141 ggacgtgcgt gtgctgcgcg ccgcgttcga cgagatcgtc cgccggcacg agtcgctccg
     1201 caccaccttc gccctgcgcg agacgccgga gaaggggcgc gaggcggcgc cggtgcaggt
     1261 gatctctccc cccgccccct tcctcctccc cctgatcgac ctctccctcg tgcccgagcc
     1321 ggcgcgcggg caggaatccc agcggctgct gtccgccgag gcgaagaccc ccttcgacct
     1381 cgcgcgcggg ccgctgctgc gggcggctct ggtgcgcctg tccccccggg accacctgct
     1441 gctcgtggac atgcaccaca tcatctcgga cggctggtcg tgcggcctgt tcttcaagga
     1501 gctgtcggac ctctacgccg cctttgccga gaagcggtcc tcccctctgc cggagctgcc
     1561 gatccaatac tccgacttcg ccgtctggca gcgcgactgg ctgcggggcg aggtgctgga
     1621 ggagcagctc cgctactggc gcgagcagct cgcgggcagc ccgccgatgc tcgacctgcc
     1681 gaccgaccgc ctgcgcccgg cggtccagac ccacaacggc ggctcggtga tccaccgctt
     1741 cccggccgac ctcacccggg ccctgcgcga gacctcgcgg cgcgaagggg cctcgctgtt
     1801 catgatcctc ctcgccggct tcaagctcat cctctcgcgc ctggccgccc aggacgaggt
     1861 ggtggtgggc gccccgagcg ccggccgcaa gcgggtcgag accgaagggc tcatcggctt
     1921 cttcctcaat acgctggtcc tgcgcacgca cctggcgggc gacccctcct tccgggagct
     1981 gctgggccgg gtcaagcggg tggtcctggg cgcctaccgg ttccaggacg tgcccttcga
     2041 gaagctcctg gaggagctgc agcccgagcg ccacctcagc cgctcgccgc tcttccaggt
     2101 gctgttcaac atgatcaccg tccccgaggc ccgcctcgac ctgccggggc tccacatcga
     2161 catgctgccg gcgcccgagc cggagtcgaa gttcgacttc acgctctacg tccaggagca
     2221 gccggacgcc ctgcagatcg acctggtcta caacgccgac ctgttcgacc gggcgcgcat
     2281 ggaggagatg ctgcgccagt tcgagcacgt catggcgcag tcggcggccg accccgagcg
     2341 gcggatcggc gactactccc tggtctcgcc ggtggcggcg gccctgttgc ccgacccggc
     2401 ggcgacgctg ggcggcgagt ggatgggggc ggtccaccac gccctctccc gtcacgccgg
     2461 cgaggacgcg gaggcgccgg cggtcaccga ctggcgcggc gagcgctgga gccgcggcga
     2521 gctcgaggcc cgctccaacc agctcgcctg gtacctccag gcgagcggcg tgcgcaccgg
     2581 cgacgtggtg gcggtctggg cgcaccgcag cgcctcgctg gtctgggccg tgatgggcac
     2641 cctcaaggcc ggcgcggcgt tcatgatcct cgacccggcg taccccacgg cgcgcctgct
     2701 cgactacctg gagatcggcc gccccgccgg ctggctggcg gtggaggggg cggcggcgcc
     2761 gcccgccgag gtggaggagg ctctgcggac ggccggttgc cgttgccggc tggagctgcc
     2821 ccccctctcg gcggcgatgg agaaggggct gctcgccaac ttcccgccgt tcgatcccgg
     2881 cgtgccggtg accgcggacg acgcggccgt catcaccttc acctccggct ccaccggcaa
     2941 gcccaagggg gtggtggggc gccacggccc gctctcccac ttctatccct ggatggcgcg
     3001 gcgcttcgag ctgtcggagg cggagcgctt cggcatgctg tcggccctgt cccacgaccc
     3061 gttgcagcgc gacctgttca cgccggtctg gctcggcgcc tcgatccacg tccccgatcc
     3121 ggagcagatc agcactcccg ggtacctgtc ggactgggcg cggagcgagg gggtcaccat
     3181 gctgcatctg acgccggcca tgatggagat cctcctcggc tcggcggagg agagggggga
     3241 ggagctcccg agcctgcgcc gcgccctggt ggtgggcgac ctcctcaagc gctccgacgt
     3301 cgcccgcctg cagcggctct gcccgaacct catggcggtc aacctctacg gctcgaccga
     3361 gacgcagcgc tcggtcgcct gcttcgaggt gccgcggccg gaggtctggc ccgccggtcc
     3421 cctggacaag ctggtgctgc cgctgggccg gggcatggag gacgtccagc tcctcatcct
     3481 caaccgcgcc cagaagctct gcggcgtcgg cgaggtgggg gagatctaca tgcgcagccg
     3541 ccatctcgcc cgcggctatc tgggcgacga ggcgctgacc acgtccaggt tcctccccaa
     3601 cccgttcgcc ccgctcgctc cggaggccga cggggaccgg gtctacaaga cgggcgacct
     3661 cggccgctac ctgccggacg gc
//
LOCUS       DASPJI010000536        12666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_780699, whole genome shotgun sequence.
ACCESSION   DASPJI010000536 DASPJI010000000
VERSION     DASPJI010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12666)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12666
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780699"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VEL74_14650"
     CDS             complement(<1..422)
                     /locus_tag="VEL74_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HYG63817.1"
                     /translation="MSMIDIDAANQTAVERMMAARPILTGLAPAREVIPGLEDGLLLH
                     AGPPIEWERASGPLRGAATGALIYEGRARDAAEAAALVERGEVRLEPCHHHAAVGPMA
                     GVISPSMQVYVIEDAVHGHRTFSNLNEGYGKVLRYGAY"
     gene            complement(419..2134)
                     /gene="fdrA"
                     /locus_tag="VEL74_14655"
     CDS             complement(419..2134)
                     /gene="fdrA"
                     /locus_tag="VEL74_14655"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HYG63818.1"
                     /translation="MSPVTRCAVRPGTYHDSVVLMRLQAALAALPGILDAGVVMATPA
                     NLALLTASGLLVEDSLSTVADDLLIAVKADSEPAAAEALSRVDALLQVRRQEVSEEHR
                     PRSLETALRSLPQARWVLVSVPGRFAASVAHDALSLGLNVFLYSDNVSLADEVALKRR
                     AHEKGLLVLGPDCGTAIVGGTGLGFANRTKPGRIGVIAASGTGLQAAVSFLHLRGFGV
                     SQAIGIGGRDLAAEVGGITAAQALDLLRRDPETEVILFLSKPPAPEVARRLIALAHAT
                     DKPVVMSLLGTSPPLLHAWNVHFSIGVEAAIEEAILCLDGKDSGAVERACSFAAGQRW
                     VRGLFSGGTLALELVARLSAVIEPVYSNVVTGAAQPIPDALHSQGHTILDLGADEFTV
                     GRLHPMIDFDLRLRRLRQEAADPEVALILLDVVLGDGAHADPAGELAPVIQRLRDVAH
                     GAGRDLDVAVVVIGTDLDPQDRKRQVEDLERAGARVCRSLIDMVDYTLHRLEEIAGGP
                     SLFADPPVPLEAVRGPVTAINVGLELFHDSLAAQGAQTVQVDWKPPAGGNEKLAGILS
                     RMKKR"
     gene            complement(2131..3366)
                     /locus_tag="VEL74_14660"
     CDS             complement(2131..3366)
                     /locus_tag="VEL74_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HYG63819.1"
                     /translation="MAGLDLDRLRDRFEQREPSVHAFVPEDGRFGRLQREMDEALAAF
                     PDSQPRGPLFGVPIGVKDVFHVNGLPTRAGSRLPPEELSGPEAEVVSRLRGLGALILG
                     KAVCTEFAFFAPGPTRNPHDPEHTPGGSSSGSAAAVAAGLCPAALGTQTIGSIIRPAA
                     YCGVVGFKPSYGRIPTAGLIPLAPSLDHVGFLTIDVQTAINLAVGLMDDYGSQGVPAR
                     PPVLGIPEGPYLSRASEEGLAHFREICLRLSSRYEVRSVPAMADFAEIEARHFRITAA
                     EAARVHQDWFPRFRDLYAVQTVDLIERGQKVPSDQLERELAGREELRGELTGLMDEHG
                     IDLWLSPPAQGPAPRGLASTGSPVMNLPWTQAGLPVLGVPAGSDAEGLPMGLQVTGRW
                     RTDELLLHWGGEIAQEVAA"
     gene            complement(3367..3876)
                     /locus_tag="VEL74_14665"
     CDS             complement(3367..3876)
                     /locus_tag="VEL74_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HYG63820.1"
                     /translation="MSRLHNVSVTVNGRRYERPAEPRLLLSDFLRHDLGLTGTHVGCE
                     HGVCGACTILVDGEPVRSCLMLAVQADGRELATVEGLTAGGQAGGNELHPLQQAFWEA
                     HGLQCGFCTPGFLMTLVPFLEENPDPSEDEIRHAISGNLCRCTGYQHIVDAVKLAAMA
                     AHGGPEKKG"
     gene            complement(3873..4769)
                     /locus_tag="VEL74_14670"
     CDS             complement(3873..4769)
                     /locus_tag="VEL74_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HYG63821.1"
                     /translation="MKPAPFDYVAPDSLEEALDILARSGGDAKLLAGGQSLIPVMNFR
                     LAQPSLLIDLNRLRGLDAIYMDSALNGPELRLGALTRQRRLERDPLIAERAPLLREAV
                     PYIAHPQIRNRGTVGGSIAHADPAAELPAVALALRARFRLQRSGGDRWVEARDFFLGL
                     FTTVLEPEEMLTEIALPPLPPRTGWSFMEVARRHGDYAQAGLAALVTLDETGRCREAR
                     LVYLSVGDAPVEASKAAALLVGEALTPERIAAAAEVASREEMDPDGDIHASAAFKRHL
                     ARVLTIRALGAAVARAQREGTR"
     gene            4891..5376
                     /locus_tag="VEL74_14675"
     CDS             4891..5376
                     /locus_tag="VEL74_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HYG63822.1"
                     /translation="MDAFQGWWQWEEFWVPLAKLIAAFFLTMPIAWEREHSTRIMGLR
                     TFSLVSMASCGYVLIANQVVGSDADPQARIIQGLMTGIGFIGGGAILKHGANVRGTAT
                     ASSIWMTGALGAAVGYSEIEVAVLLSGLNYLTLRVLTPLGERRAESRQTPSGEDDPED
                     S"
     gene            5392..7170
                     /locus_tag="VEL74_14680"
     CDS             5392..7170
                     /locus_tag="VEL74_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HYG63823.1"
                     /translation="MTFEIFVVLAILAGAVACFATERLPVDLVALLVLSALLASGILT
                     PEQAVSGFSHTATVTVAAMFVLSAGLARTGVVGLLGQRLGAVARLGLRPALLALMALI
                     GVVSAFINNTAAVAIFLPIVLSLARDMGTSPSKLLIPLSFASMFGGVCTLIGTSTNIL
                     VSSIAEQHGEAPFGMFELSRLGLVLFAAGGLYMMTAGIRIIPDRRGGADLAQSYGMGD
                     YLTEILVLPESPAVGKPLSESPLARDLDLDVLEILRAGRRLADPGPRTVLAAGDLLRV
                     RCDVQKIRLAEQQKGIVLKSGLKWRDEDSAPSEAALLEAVIAPGSALVGKTLKEAGFR
                     QIYGGTALAIRHRGEIMRERLGTTRLRPGDVLLVEVRRDRMAALQHNPDFVVVSEVSL
                     DEPRRGKLLPAVIIVAAVVATAALGLLPIVGSAILGSVLLVLTRCLTLAEAYQAIEWR
                     IIFLLGGILPLGIALETSGAALLLSSLLVETVGIWGPVALVSVFYLLTSLLTEAMSNN
                     ATAALLAPIAISTAAALDVDPRPFLVAVTFAASASFMTPVGYQTNTLIYGPGHYRFTD
                     FLRVGTPLNLLFWLLATLLIPWFWSF"
     gene            7199..8689
                     /locus_tag="VEL74_14685"
     CDS             7199..8689
                     /locus_tag="VEL74_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HYG63824.1"
                     /translation="MTPESSAARPLTDRLREMRRAPGDLYELRQPPTDPRLSNADLAP
                     TSVTQRTWTTYHVASLWVGLAVCIPTYILAAGMVDGGMSWWQAMLTILLGNLIVLVPM
                     VLIAHAGTRFGIPFPVLARASFGTSGAHVPSILRGLVACGWFGIQTWIGGQAIHTMLR
                     TAFPAWERVPGGVWIAFFAFWLWNMYIVVKGSNSIKRLEAWAAPFLLVAGLALLAWAA
                     SRAGGLGPILARPSRFQTAGEFFAFFVPSLTAMVGFWATLALNIPDLSRYARDQKAQA
                     AGQLLGLPTTMTLFSFVGVAVTSASVIIFGEAVWDPVALLSRLGNPFMIVASLFALLI
                     ATLTTNVAANVVAPANGLANLWPSRITFAIGGLITGVLGILIMPWRLLSDYGTFIFGW
                     LVGYSGLLGPIAGIFIADYWLVRKARLSLADLYRADGLYGRWNPKALAALGAGVAIAL
                     IGLVVPALRVLYDYAWFVGFGVAFVLYAALMRGTPVLDLSQVSDER"
     gene            8782..9720
                     /locus_tag="VEL74_14690"
     CDS             8782..9720
                     /locus_tag="VEL74_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG63825.1"
                     /translation="MANKFKPIDQQVIVITGASSGIGLATAVAAAQQGAKLVLASRSG
                     QTMEDLVQRMTAEGVEAVAVTADVGDRRQVEQIAQVAIGRFGGFDTWINDAGVSIYGR
                     LEEVSEEDSRRLFDTNFWGVVNGSLAALPHLKREGGVLINVGSEVSDAVVPLQGMYSA
                     SKHAVKGFNDALRVEVEEIDKAPVAIVLVQPTAVDTPFPQHAKNYMDQEPKLPTPMIE
                     PEKVAQAILDAAVKPERYVRVGAMSKLNTTVAKIAPGLGDKLSSKQADRQQYNEPPRD
                     PEGTLYKPGEEGRIHGEGPSSHKKETVEEPELAHRS"
     gene            9840..11108
                     /locus_tag="VEL74_14695"
     CDS             9840..11108
                     /locus_tag="VEL74_14695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYG63826.1"
                     /translation="MEPYENNHKQRRFLIIAIMALVVVLAGVFFLKRGKDEGGEQYRS
                     EAASRGDITATASATGTLSAVTTVQVGSQVSGIVAKLHADFNSQVQQGQLLAELDPTS
                     FLQAVEQRQAEVTRSQIEVANAQLTYNRQRRLAASGLVAQAEVDSARAAYESARAQLS
                     LSQASLKQAKTNLGYTKIYSPIEGQVVDRQYDIGQTVAASFQAPTLFTIAKDLTKMQV
                     QAAIDQSDIGRIKVGDPVRFTVDAYPDQQFRGAISQVRLNATVNQNVITYPVIVEVAN
                     PDLKLRPSMTADVTIEVDEVQNVLRVPNAALRFRPETEEGQGQQQGQGQRRAGAGSGN
                     AGSGGAGRQGRQSGQTVYVLEKSEDGKSELRPVRITTGLTDGRYTEVKSVLSGELSQG
                     DTVVTGLATLTNPQSGTAAGGRMGPRMGRF"
     gene            11111..11947
                     /locus_tag="VEL74_14700"
     CDS             11111..11947
                     /locus_tag="VEL74_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG63827.1"
                     /translation="MSDPVIQIRDLKKVYRMGEVEVNALAGIDLSVEPGELVAIMGPS
                     GSGKSTLMNILGCLDRPSSGTYLLDGVDVSRLDRDRRAEIRNSKIGFVFQSFNLLART
                     RATENVELPMLYSDLGFSTAERHRRARQALARVGLAGREDHYPSQLSGGQQQRVAIAR
                     ALVTDPPLILADEPTGNLDSRTSEEIMGIFQELNDAGKTVVLITHETDIAAHAKRVIY
                     LRDGLIWRDEKIVQTRSKPGAAVTESELAIAFPFQLAPSSAGTAPTPVEVEAEELEEV
                     LR"
     gene            11944..>12666
                     /locus_tag="VEL74_14705"
     CDS             11944..>12666
                     /locus_tag="VEL74_14705"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG63828.1"
                     /translation="MKITALRLLNIAKVGLMAISRNKTRSLLTMLGIIIGVGCVITML
                     AVGTGASRAIQSTISSLGTNFIMLFPGAETQGGARIAGESRITVEDVNAIREEAPSVA
                     YVSPSVRESAQVVAGDLNWGTSVYGVDVDWPFIRAWNVADGDFFTDVDVRTSARVAVL
                     GATVADNLFPAGDAIGSSIRIKNVPFRVVGVLDRKGGNMMGQDQDDQIIAPFTTVMKQ
                     LQGRERIGQVLISARSESLVGQA"
BASE COUNT         1936 a         4396 c         4347 g         1987 t
ORIGIN      
        1 ctgtaggcgc cgtagcgcaa aacctttcca tatccctcgt tgaggttgga gaaggtgcgg
       61 tgtccgtgca cggcgtcttc gatcacgtag acctgcatcg agggggagat cacgcccgcc
      121 atggggccga ccgccgcgtg atggtggcag ggctccaggc gcacctcgcc gcgttcgacc
      181 agcgccgccg cctccgccgc gtcgcgggcc cgtccctcat agatcagcgc gccggtcgcg
      241 gcgccgcgca gcgggcccga ggcccgctcc cactcgatgg gcgggccggc gtggagcagc
      301 aggccatcct ccaggcccgg gatcacctcg cgcgccggag ccaggccggt gaggatcggc
      361 cgcgcggcca tcatgcgctc gacggcggtc tggttggcgg cgtcgatgtc gatcatgctc
      421 atcgtttttt catccggctc aggattccgg ccagcttctc gttgccgccc gccggaggtt
      481 tccaatcgac ctggaccgtc tgggcccctt gggcggcgag gctgtcatga aatagctcca
      541 gcccgacgtt gattgccgtc actgggccgc gcaccgcttc caacggcacc ggagggtccg
      601 cgaagagcga aggtccgccg gcgatctcct ccaaacgatg gagcgtgtag tcgaccatat
      661 cgattaacga acggcagacc cgcgcgcccg cccgttccag atcctcgacc tgtcgctttc
      721 gatcctgcgg gtccaggtcc gtcccgatca ccaccacggc cacatccagg tcgcggccgg
      781 cgccgtgggc tacgtcccgc aggcgctgga tcaccggcgc cagctctccg gccggatccg
      841 cgtgcgctcc atccccgagg accacgtcga ggaggatcag cgccacctcg ggatcggcgg
      901 cctcctggcg cagccggcgc aggcgcaggt cgaaatcgat cataggatgc agccggccga
      961 cggtgaattc gtccgcgccg aggtcgagga tcgtgtgccc ttggctgtgg agggcgtccg
     1021 ggatgggctg ggccgcgccg gtgacgacat tcgaatagac cggttcgatc actgccgaca
     1081 gccgcgccac cagctccaga gccagagtgc cacctgaaaa caggccgcgc acccaacgct
     1141 gtcccgcggc gaagctgcac gcccgttcga ctgctccgga atctttgccg tccaggcaca
     1201 ggatcgcctc ttcgatagcc gcctccacgc cgatggaaaa gtggacgttc caggcatgga
     1261 gcaggggcgg cgaggtgccg agaaggctca tgaccaccgg cttgtccgtc gcgtgcgcga
     1321 gagcgatgag ccgccgggcg acctcgggcg ccggaggctt ggacagaaaa aggatgacct
     1381 ccgtctccgg atcgcggcgc agcaggtcga gcgcctgggc cgccgtgatt cccccgacct
     1441 cggccgcgag gtcccggccg ccgatgccga tcgcctggga gaccccgaaa ccccgaaggt
     1501 ggaggaagct gaccgccgcc tggagaccgg tgccggaggc ggcgatgacg ccgatcctcc
     1561 cgggcttggt gcggttcgca aagccgagcc cggtgccgcc cacgatggcg gtgccgcagt
     1621 ccgggccgag gacgagcagc cccttctcgt gggccctgcg tttgagcgcc acctcgtcgg
     1681 ccagtgacac attgtcgctg tagagaaaaa cgttcagacc gaggctcaac gcgtcgtgcg
     1741 ccacactggc ggcgaaccgc ccgggcaccg agaccaggac ccagcgcgcc tgggggagac
     1801 tcctcagcgc agtctccagg ctgcgtggcc ggtgctcctc cgaaacctcc tggcggcgca
     1861 cctggagcag ggcgtcgacc cgcgacagcg cttccgcggc ggccggctcg ctgtccgcct
     1921 tgaccgcgat cagcaagtcg tcggcgacgg tggatagcga gtcctccacc agcagaccgc
     1981 tcgccgtcag cagcgccagg ttggcggggg tggccatgac cacgccggcg tcgaggatac
     2041 cgggcagcgc cgcaagcgcc gcctggagac gcatcaggac caccgaatcg tgataggtgc
     2101 cggggcggac ggcacagcgc gtgaccgggc tcacgcggcc acctcctgag cgatctcgcc
     2161 gccccagtgg agcaggagct cgtccgtccg ccagcgtccg gtgacctgga gccccatggg
     2221 cagcccctcg gcgtcgctgc ccgccggcac gccgaggacg ggcagcccgg cctgcgtcca
     2281 ggggaggttc atgaccgggc tgccggtgct ggcgaggcca cgcggggcgg gaccctgggc
     2341 gggcggcgac agccagagat cgataccgtg ctcgtccatg aggcctgtca gctcgccgcg
     2401 cagctcttcc cggccggcca gttcccgctc caattgatca gaggggacct tctggccgcg
     2461 ttcgatgagg tccaccgtct gaaccgcgta cagatcacga aagcgcggga accagtcctg
     2521 gtggacccgc gcggcctcgg ccgccgtgat gcggaaatgt cgcgcttcga tctcggcgaa
     2581 atccgccatg gctggcactg accgcacctc gtagcggctg gacagccgga ggcagatctc
     2641 gcggaagtgg gccaggccct cctcggaggc gcgggagagg tacggtcctt cggggatccc
     2701 cagcaccggc gggcgcgctg gcactccctg cgatccgtag tcatccatga ggcctacggc
     2761 gaggttgatc gcggtctgga catcgatggt gagaaacccg acgtgatcga gcgacggggc
     2821 gagggggatc aggcccgcgg tggggatccg gccgtagctg ggcttgaaac cgaccacacc
     2881 acaataggcc gcgggccgga tgatcgatcc gatcgtctgc gtgccgagcg ccgccgggca
     2941 gaggcccgcc gccaccgccg ccgcggagcc gctactcgac ccgcccgggg tgtgctccgg
     3001 gtcatgcggg ttgcgcgtcg gtccgggggc gaagaaggcg aattcggtgc agacggcctt
     3061 gcccagaatc agcgcgccga ggccgcgcag ccgcgagacc acctccgcct ccggtccgga
     3121 cagctcttcg ggcggcaagc ggctgccggc ccgcgtcggg aggccgttca catgaaagac
     3181 gtccttgacg ccgatgggaa cgccgaacag gggcccgcgg ggctgagagt ctggaaaggc
     3241 cgcgagcgcc tcgtccatct cccgctggag gcgcccgaag cggccgtcct ccggaacgaa
     3301 ggcgtggacg gagggctcgc gctgctcgaa ccggtcccgc aggcgatcca ggtccagccc
     3361 ggccatttat ccctttttct ccgggcctcc gtgagcggcc attgcggcca gcttcacggc
     3421 gtcgacgatg tgctgatagc cggtgcaccg gcacaggttg cccgagatcg cgtgccggat
     3481 ctcgtcctcg ctcgggtcgg ggttctcttc gaggaacggc accagggtca tcaggaaccc
     3541 gggggtgcag aagccgcact gcagcccgtg cgcctcccag aacgcctgct gcaacggatg
     3601 caactcgttg ccccctgcct gtcctccggc ggtgaggcct tcgacggtgg ccagctcgcg
     3661 gccgtcggcc tggaccgcca gcatcaggca ggagcggacc ggctcgccgt ccaccaggat
     3721 cgtgcaggcg ccgcagacgc cgtgctcgca gccgacatgg gtgccggtga ggccgaggtc
     3781 gtggcgcagg aaatccgaca gcagcagccg cggctcggcc ggccgttcgt agcgccggcc
     3841 gttgacggtg acggagacgt tgtggagtcg gctcatcggg tcccctcccg ctgggcccgg
     3901 gccacggcgg cgccgagcgc ccggatggtc aagacacgcg ccaggtggcg cttgaacgcg
     3961 gcgctggcgt gaatgtcgcc gtccgggtcc atctcctcgc gcgaggcgac ctcggccgcc
     4021 gcggcgatcc gctccggtgt caacgcctcg ccaaccagga gggcggccgc cttggacgcc
     4081 tcgaccgggg cgtcgccgac gctcagatag accagccgcg cctcgcggca gcggcccgtc
     4141 tcgtcgagcg tcaccagggc ggcgagtccg gcctgcgcgt agtcgccgtg acgccgggcg
     4201 acctccatga aggaccagcc ggtgcgtggc ggcaggggag ggagggcgat ctcggtcagc
     4261 atctcctccg gctccaggac cgtggtgaag aggccgagga agaagtcccg cgcctcgacc
     4321 cagcgatcgc cgccggagcg ctgcaggcgg aaccgggcgc ggagggccag cgcgaccgcc
     4381 ggcagctcgg ccgccgggtc cgcatgggca atgctgccgc cgaccgtgcc gcggttgcgg
     4441 atctgcggat gggcgatgta ggggacggcc tcgcggagca gcggcgcccg ctcggcgatc
     4501 aggggatcgc gctccaggcg gcgctgccgg gtcagggcgc cgaggcgcag ctccgggccg
     4561 ttcagggcgc tgtccatgta gatggcgtcc agcccgcgga gccgattgag gtcgatcagc
     4621 agggacggct gcgccagcct gaagttcatg accgggatca ggctctggcc gccggccagg
     4681 agcttggcat ctccgccgct ccgtgccagg atgtccagcg cctcctccag cgaatcggga
     4741 gccacgtagt cgaacggggc tggtttcatc cggctctccc tcgaaggtga gggaggattc
     4801 taccggaaat ggaggtgagc tcggccgggc gctccccgca tgggctttgc atacctccgg
     4861 ccatggctct ggagagagga ggaccggcgg atggatgcgt ttcaagggtg gtggcagtgg
     4921 gaggagttct gggtgccgct ggccaagctg atcgcggcgt tcttcctgac catgcccatc
     4981 gcctgggagc gcgagcattc cacccgtatc atggggctcc gcaccttctc cctggtgtcg
     5041 atggccagtt gtggctatgt gctgatcgcg aatcaggtgg tgggctccga cgccgacccg
     5101 caggcgcgga tcattcaggg gctgatgacg gggatcggct tcatcggcgg cggcgccatc
     5161 ctcaagcacg gtgccaacgt ccgcggcacc gcgacggcct ccagcatctg gatgacaggg
     5221 gctctggggg ctgcggtcgg ctactccgag atcgaagtgg cggtcctgct cagcggtctc
     5281 aactatctga cgctgcgggt gttgacgccg ctcggcgaac ggcgtgccga gtcccggcag
     5341 acgccctcgg gggaggatga tccggaagac agttgaagct gccggcgcgc catgaccttc
     5401 gagatcttcg ttgtcctcgc catcctcgcc ggcgccgtgg cctgctttgc caccgagcgc
     5461 ctgccggtgg atctggtggc gctgctggtg ctgtcggccc tcctcgccag cggcattctc
     5521 acccccgagc aggccgtttc cggcttcagc catacggcca ccgtgacggt ggccgccatg
     5581 ttcgtcctga gcgccgggct ggcgcggacc ggtgtggtcg gcctcctcgg ccagcggctg
     5641 ggagccgtgg cgcgtctcgg cctccgtccg gcgctcctcg ccctcatggc cctcatcggc
     5701 gtcgtctccg cgttcatcaa caacacggcg gcggtggcca tcttcctccc catcgtcctc
     5761 agcctcgccc gggacatggg aaccagcccg tcgaagctgc tgatcccgct ctccttcgcc
     5821 tcgatgttcg gcggcgtctg cacgctgatc ggaacctcca ccaacatcct ggtcagctcc
     5881 atcgccgaac agcacggcga ggcgccgttc gggatgttcg agctctcccg gctcgggctg
     5941 gtgctgttcg ccgccggcgg gctctacatg atgacggccg gcatccgcat cattcccgac
     6001 cggcgcggcg gcgcggatct ggcccagtcc tatggcatgg gggactatct gaccgagatc
     6061 ctggtgctcc ccgagtcccc ggcggtcggc aagcccctgt ccgagtcccc cctggcccgt
     6121 gacctggacc tggacgtgct ggagatcctt cgcgccggcc ggcggctggc cgaccccggg
     6181 ccccggacgg tgctggcggc gggcgacctg ctccgggtgc gctgcgacgt ccagaagatc
     6241 cgccttgccg agcagcagaa agggatcgta ctgaagtcgg gcctcaaatg gcgcgacgag
     6301 gactctgccc cctcggaggc cgcgctgctc gaagcggtca tcgcgcccgg atcggcgctg
     6361 gtggggaaga cgctcaagga ggcgggcttc cgtcagatct atggcgggac ggcgctcgcc
     6421 atccgccatc gcggggagat catgcgagaa aggctgggca ccacccggct gcgcccgggc
     6481 gacgtgctgc tggtcgaggt gcggcgcgac cgtatggcgg cgctgcagca caatcccgac
     6541 ttcgtggtgg tgtccgaagt cagtctcgac gagccgcggc ggggcaagct cctccccgcc
     6601 gtgatcatcg tcgccgccgt ggtcgccacg gcggcgctgg ggctcctgcc tatcgtgggg
     6661 agcgccatcc tcggctcggt gttgctggtg ctcacccgct gcctgacgct ggccgaggcc
     6721 taccaggcta tcgagtggcg gatcatcttc ctgctcgggg gtatcctgcc cctgggcatc
     6781 gccctggaga ccagcggcgc ggcgctcctg ctgtcgtccc tgctggtcga gacggtgggg
     6841 atctggggac cggtggcgct ggtatccgtc ttctatctcc tcacctccct gctgaccgag
     6901 gcgatgtcga acaacgcgac ggcggcgctg ctcgcgccca tcgccatcag caccgccgcg
     6961 gcgctcgacg tcgatccccg gccttttctc gtcgccgtga ccttcgccgc gtcggcgagc
     7021 ttcatgacgc ccgtgggcta tcagaccaac acattgatct atggcccggg gcactaccgg
     7081 ttcaccgatt tcctgcgcgt cggcactccg ctgaacctgc tcttctggct gttggcaaca
     7141 ctgctcatcc cgtggttctg gagcttctga atatcactgg ggtacgatag gcctccagat
     7201 gactccagaa agctctgccg cgcgtccgct gacggaccgt ctgcgggaga tgcgccgcgc
     7261 tccgggagac ctctacgagc tgcggcagcc tcccacggac ccgcgtctgt cgaacgccga
     7321 cctcgctccc acctccgtca cacaaaggac ctggaccacc taccacgtgg cgtcgctgtg
     7381 ggtcggcctg gcggtctgca tccccaccta catcctggcg gcgggcatgg tggacggcgg
     7441 gatgagctgg tggcaggcga tgctcaccat cctgctcggc aacctgatcg tgctcgtccc
     7501 catggtgctc attgcccacg ccgggacgcg cttcggcatc cccttcccgg tgctggcgcg
     7561 cgcctccttc ggcacctcgg gggcgcacgt gccgtcgatc ctgcgcggcc tcgtggcctg
     7621 cggctggttc ggcatccaga cctggatcgg cggccaggcg atccacacca tgctgcggac
     7681 cgccttcccg gcctgggagc gggtacccgg cggcgtctgg atcgccttct tcgccttctg
     7741 gctgtggaac atgtacatcg tggtgaaggg gagcaactcg atcaagcggc tggaggcctg
     7801 ggcggcgccc ttcctcctgg tcgccgggct ggcgctcctc gcctgggcgg cgagtcgggc
     7861 cggcggactg gggccgatcc tcgcccgccc gagccgcttc cagacggcgg gcgaattctt
     7921 cgccttcttc gtcccctccc tcaccgcgat ggtggggttc tgggccacgc tcgccctgaa
     7981 catccccgat ctgtcgcgct atgcccgcga tcagaaggcc caggccgctg gtcagctcct
     8041 cggcctgccg accacgatga ccctgttctc gttcgtcggc gtcgccgtca cctcggcctc
     8101 ggtaatcatc ttcggcgagg cggtctggga ccctgtggcc ctgctctcgc ggctcggcaa
     8161 cccgttcatg atcgtcgcct cgctgttcgc actgctgatt gccacactga ccaccaacgt
     8221 ggccgcgaac gtggtcgccc cggccaacgg gctcgccaac ctctggccgt cgcggatcac
     8281 ctttgccatc ggcggcctca tcaccggcgt cctcggcatc ctgatcatgc cgtggcggct
     8341 cctctcggac tacggaacct tcatcttcgg ctggctggtg ggctactcgg gccttctcgg
     8401 accgatcgcg gggatcttca tcgccgacta ttggctggtc cgcaaggcgc gcctctccct
     8461 cgccgacctc taccgggcgg acggcctcta cggccgctgg aacccgaagg cgctggccgc
     8521 gctgggggcc ggagtcgcca tcgctctgat cggcctggtg gtccctgcgc tccgcgtcct
     8581 ctacgactat gcctggttcg ttggcttcgg ggtggcgttc gtgctctacg cggcgctcat
     8641 gcgcgggacg ccggtactgg acctgagcca ggtgtcggac gagcgctgac accaaggcat
     8701 caaggcctaa cggcacggtg gttgcaccct agggagggtg ggggcggatc ggaccgccgg
     8761 cagatcacca aggaggaact catggccaac aagttcaaac ccatcgacca gcaggtcatc
     8821 gtcatcaccg gtgcctccag cggcatcggc ctcgccacgg ccgtggccgc cgcccagcag
     8881 ggcgccaagc ttgtcctggc gtcgcgcagc gggcagacaa tggaagatct cgtacagcgg
     8941 atgaccgccg agggcgtcga ggccgtcgcg gtgacggccg acgtcggcga ccgccggcag
     9001 gtcgagcaga tcgcccaggt cgccatcggc cgcttcggcg gcttcgatac ctggatcaac
     9061 gacgccggcg tctccatcta cggacgtctc gaggaggtca gcgaggagga cagccgccgc
     9121 ctcttcgaca ccaacttctg gggcgtcgtg aacggatcgc tcgccgcgct gccgcacctc
     9181 aagcgtgagg gcggcgtgct gatcaacgtc ggcagcgagg tctcggacgc ggtggtgccg
     9241 ctccagggca tgtactcggc ctcgaagcac gcggtcaagg ggttcaacga tgccctccgg
     9301 gtcgaggtcg aggagatcga caaggcgccg gtggcgatcg ttctcgtcca gccgacggcg
     9361 gtcgataccc ccttcccgca gcacgccaag aactacatgg accaggagcc gaagctcccg
     9421 acgccgatga tcgagccgga gaaggtcgcc caggcgatcc tcgacgccgc cgtgaagccg
     9481 gagcgctacg tcagggtggg ggcgatgtcc aagctcaaca ccacggtggc gaagatcgcc
     9541 cccggcctgg gggacaagct ctcctccaag caggccgacc ggcagcagta caacgagccg
     9601 ccgcgggacc ccgaaggcac cctctacaag cccggggagg agggccgcat ccacggcgag
     9661 ggcccgtcca gccataagaa ggagacggtg gaggagcctg agctggctca tcgctcctag
     9721 gccggcctgt gtcagcgcca ggaatgtttc ctgaacggga attttccggg acttgtaaag
     9781 aataggttaa gaatgggccg gccgggaaac ctcggccggc ccctcacgtt tggaaagtca
     9841 tggaacctta cgagaacaac cacaagcagc gacgttttct catcatagcg atcatggcgc
     9901 tggtggtggt cctggccggc gtgttcttcc tgaagcgcgg gaaggacgag ggcggtgagc
     9961 agtaccgctc ggaagcggcg agccggggag acatcacggc caccgcgagc gccaccggaa
    10021 ccctctcggc ggtgaccacc gtccaggtgg gaagccaggt ctccggcatc gtcgccaagc
    10081 tccacgccga tttcaacagc caggtccagc aggggcagct cctcgccgag ctcgacccga
    10141 cgtctttcct gcaagcggtg gagcagcgtc aggccgaggt cacccggtcg cagatcgagg
    10201 tggccaacgc ccagctcacc tacaaccggc agcggcgcct agccgcctcc ggcctggtcg
    10261 cccaggccga ggtcgactcc gccagggccg cctacgagtc ggcccgcgcc cagctctccc
    10321 tgtcgcaggc ctcgctgaag caggcgaaga caaacctcgg ctataccaag atctactcgc
    10381 ctatcgaggg ccaggtggtc gaccgccagt acgacatcgg tcagacggtc gccgcctcct
    10441 tccaggcgcc gaccctgttc acgattgcca aggacctcac caagatgcag gtgcaggccg
    10501 cgatcgacca gtccgacatc ggccgcatca aggtcggcga tcccgtgcgc ttcacggtgg
    10561 acgcctaccc ggaccagcag ttccggggcg cgatctccca ggtgcgcctg aacgccacgg
    10621 tcaaccagaa cgtcatcacc tacccggtaa tcgtcgaggt ggcgaacccc gatctgaagc
    10681 tgcgcccgag catgaccgcc gacgtcacca tcgaggtgga tgaggtccag aacgtgctgc
    10741 gcgtgcccaa cgccgccctg cggttccggc cggagacgga ggaggggcag gggcagcagc
    10801 agggacaggg gcagcggcgc gcgggtgccg gaagcggcaa tgccggaagc ggcggtgcgg
    10861 gacggcaggg ccggcagtcc gggcagaccg tctacgtgct cgagaagtcg gaggacggca
    10921 agagcgagct gcggccggtc cggatcacca ccggcctcac cgacggccgc tacaccgagg
    10981 tgaagtcggt gttgtccgga gagctgagcc agggcgacac ggtggtcacc ggcctggcga
    11041 cactcaccaa cccacagagc gggaccgccg ccggcgggcg tatgggaccg cgcatgggac
    11101 ggttctgatc atgagcgatc cagtcatcca gatccgagat ctcaagaagg tctaccggat
    11161 gggcgaggtc gaggtcaacg ccctcgcggg gatcgacctg tcggtcgagc ccggcgagct
    11221 cgtggcgatc atggggccct ccggctccgg caagtcgacg ctgatgaaca tcctcggctg
    11281 cctcgaccgc cccagctccg gcacctatct cctcgacggc gtcgacgtgt cgcgtctcga
    11341 ccgcgaccgc cgggccgaga tccgcaactc gaagatcggc ttcgtgttcc agagcttcaa
    11401 cctgctggcg cggacgcggg cgacggagaa cgtggagctg ccgatgctct acagcgacct
    11461 cgggttctcc accgcggagc gccaccggcg cgcccgccag gccctcgccc gggtcggtct
    11521 cgccggccgc gaggaccact atccgtccca gctctccggc ggccagcagc agcgcgtcgc
    11581 catcgcccgc gccctggtca ccgatccgcc gctcatcctc gcggacgagc ccaccggcaa
    11641 cctcgactcg cgcacttcgg aagagatcat gggcattttc caggagctca acgacgccgg
    11701 caagaccgtg gtgctgatca ctcacgagac cgacatcgcg gcgcacgcca agcgtgtgat
    11761 ctatctgcgg gacggcctga tctggcgcga cgagaagatc gtccagacgc ggtcgaaacc
    11821 cggggccgcg gtgaccgagt ccgagctcgc catcgccttc cccttccaac tcgcgccgtc
    11881 ctccgcgggg acggcgccga cgccggtgga agtggaggcg gaggagttgg aggaggtgct
    11941 gcgatgaaga tcaccgccct gcgtctcttg aacatcgcga aggtcgggct gatggcgatc
    12001 tcgcgcaaca agacgcgctc gctgctcacc atgctgggaa tcatcatcgg cgtgggctgc
    12061 gtcatcacca tgctcgccgt gggcaccggc gcctcgcgcg ccatccagtc gacgatcagc
    12121 tcgctgggta cgaacttcat catgctcttc cccggcgccg agacgcaggg aggcgcgcgg
    12181 atcgccggtg agtcccggat cacggtcgag gacgtgaacg cgatccggga ggaggccccg
    12241 tcggtggcct atgtctcgcc ctcggtgcgc gaatcggcgc aggtggtggc gggcgacctc
    12301 aactggggta cctcggtcta cggcgtggac gtcgactggc cgttcatccg cgcctggaac
    12361 gtggcggatg gcgacttctt caccgacgtc gacgtccgga cctcggcccg tgtggccgtg
    12421 ctcggggcga cggtggccga caacctcttc ccggcgggcg acgcgatcgg ctcctcgatc
    12481 cgcatcaaga acgtcccctt ccgggtggtc ggcgtgctcg accgcaaggg tgggaacatg
    12541 atggggcagg accaggacga ccagatcatc gctccgttca ccacggtgat gaagcagctc
    12601 cagggccgcg agcggatcgg ccaggtgctg atctcggcgc gctcggaatc cctggtgggg
    12661 caggcg
//
LOCUS       DASPJI010000537         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_843820, whole genome shotgun sequence.
ACCESSION   DASPJI010000537 DASPJI010000000
VERSION     DASPJI010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843820"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="VEL74_14710"
     CDS             complement(<1..1346)
                     /locus_tag="VEL74_14710"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HYG63829.1"
                     /translation="MATATKEPTTRPEAQTATPAGGARVTIPVSGMTCAGCQAGVQKA
                     LERQPGVRDANVNLMMKSAAVTYDPAVTSPQSLVEAIRETGYGAELPRPEVTAFQEQE
                     DRDRAQEEEYRELRLKAIVSGIAGALAMLLSMPLMVAGAQGLAGHAPVADPFMRWAME
                     SMSPALERVLPWLYRLDPAVLSYGLLALTVGIMGWAGRHFYTRAWSGFRHHSADMNTL
                     IAVGTGAAFIYSVVATVAPGFFLRHGMPPDVYYEAVIIIIALILTGNTLEARAKSRTS
                     SALRGLAALQPKTARVVRDGNEVDIPVEQVMSGDTVVVRPGERVPVDGQVLSGASAVD
                     ESMLTGESMPVEKGPGAAVIGGTINRTGAFRYRATTVGAESVLSRIVQLMRDAQGSRA
                     PIQRLADRISAVFVPVVLSIAIATFVVWFVTAGEAPAVRGLAAAIAVLIIACPCAMG"
     gene            complement(1357..1737)
                     /locus_tag="VEL74_14715"
     CDS             complement(1357..1737)
                     /locus_tag="VEL74_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HYG63830.1"
                     /translation="MSPIDWIVIAAGLAAIAWVNWWFFLARRPAATAATIAGGVQEVT
                     IAVQGGYSPSEVRVRKGSPVRLVFDRRETSSCSEEIVLPEFGIRKFLPAFQKTVVELT
                     PEKAGSFDFTCGMSMLHGRLIVEG"
     gene            2255..2845
                     /locus_tag="VEL74_14720"
     CDS             2255..2845
                     /locus_tag="VEL74_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63831.1"
                     /translation="MSLRGFLLVALLTGPLSFSGSAFAQRSEARDGARELALGREAYA
                     RGSYPEAVGHLSQAVQALGGGGEREALADAYLRLGMAHLIGLGAPAQALPAFLSSAEL
                     AGEPATAYLWAALAAEKLGQREVSERLEAKALAAAVPVPAPAPVQTPAAAPAAPREEP
                     ARAPEVKETEAEKVDAFQYFFGKKKQKPPAEKPPGL"
     gene            complement(2855..>3233)
                     /locus_tag="VEL74_14725"
     CDS             complement(2855..>3233)
                     /locus_tag="VEL74_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG63832.1"
                     /translation="AGRPLRVLVVEDNRDAAETLGDLLRIFGHEVELAHTGPDGIEAA
                     RGFHPEVVLCDIGLPGMDGYAVAEQLRSDPDTTRCRLVALTGYGRDSDRRRAEEAGFD
                     LHLVKPVEPEQLQRLLETWSAGG"
BASE COUNT          490 a         1117 c         1128 g          498 t
ORIGIN      
        1 cccatggcgc aggggcaggc gatgatcagc acggcgatgg ccgcggcaag gccccgcact
       61 gccggcgcct cgcccgcggt cacgaaccag acgacgaagg tggcgatggc gatggagagg
      121 accaccggta cgaagacggc gctgatccgg tcggccaagc gctggatcgg cgcccgggag
      181 ccctgggcgt cgcgcatgag ctggacgatc cgggagagga cgctctcggc cccgacggtc
      241 gtcgcccggt accggaacgc accggtgcgg ttgatggtgc cgccgatgac cgccgcgccc
      301 ggacccttct cgaccggcat ggactcgccg gtgagcatcg actcgtccac ggcgctggcg
      361 ccggagagga cttgaccgtc gaccggcacc cgctcacccg gccggaccac caccgtatcg
      421 ccgctcatta cctgctccac cgggatgtcc acctcgttgc cgtcccgcac cacccgtgcg
      481 gtcttgggtt gcagggcggc gaggccacgc agggccgacg aggtgcggct cttggcccgc
      541 gcctccaggg tgttgccggt gaggatcagg gcgatgatga tgatcacggc ttcgtagtag
      601 acgtcgggcg gcatgccgtg gcgcaggaag aaccccggcg ccaccgtggc cacgacggag
      661 tagatgaagg cggcgccggt gccgacggcg atcagggtgt tcatgtccgc cgagtggtgc
      721 cggaagccgg accaggcgcg ggtatagaaa tgccgccccg cccagcccat gatccccacg
      781 gtcagggcca gcagcccata ggagaggacg gccgggtcga gacgatagag ccaggggagg
      841 acccgctcca aggccgggct catggactcc atggcccagc gcatgaacgg gtccgctacc
      901 ggagcgtgcc ccgccagccc ctgggcgcct gccaccatga gcggcatgga gaggagcatg
      961 gcgagcgcgc cggcgatgcc gctgacgatc gccttgagac ggagctcccg gtactcctcc
     1021 tcctgcgcgc ggtcgcggtc ctcctgctcc tggaacgcgg tgacctccgg ccgcggcagc
     1081 tcggcgccgt agccggtctc gcggatcgcc tccaccagcg actgcgggct ggtgaccgcc
     1141 gggtcgtagg tcaccgccgc gctcttcatc atcagattga cgttggcgtc ccgcacgccg
     1201 ggctggcgct caagcgcctt ctggaccccg gcctggcagc cggcgcaggt catccccgag
     1261 accgggatgg tgaccctggc gccgccggcc ggggtggcgg tctgtgcctc cgggcgggtc
     1321 gtgggctctt tcgttgccgt agccatggct ctctcctcat ccttcgacga tgagccggcc
     1381 gtggagcatg ctcatgccgc aggtgaaatc gaagctcccg gccttctccg gggtcagctc
     1441 gaccaccgtc ttctggaagg cgggcaggaa tttgcggatg ccgaactcgg gcaggacgat
     1501 ctcctcggag caactcgagg tctcccggcg gtcgaacacc aggcggacgg gagacccctt
     1561 gcgcacccgc acctccgagg ggctgtaacc cccctggacg gcgatggtca cctcctggac
     1621 gccgcccgct atggtcgcgg cggtggcggc cgggcgccgg gccaggaaga accaccagtt
     1681 gacccaggcg atggccgcga ggccggctgc gatgacgatc caatctatcg ggctcatgat
     1741 cgactcctcg gttccggggg ctcggttccc cacttcactc attgaaacca gtataccccc
     1801 ccaccctatt cctgtatgcc ccctgtccct ctccttgatg gacgggcgag cggggcccgc
     1861 ctcttcatcc cggatcgaaa agaacgagcg ctccgtcgcc gcggcagcgg cggtggggat
     1921 cattcctgat ggggcagccg ctgcccctgg aggaatggtc atcattcctg ccggggccat
     1981 cgttgcccca cgaggaatgg tcatcattcc tgtcggggcg atcgctgccc catcaggaat
     2041 ggcgatcatt cctgtcgggg cagtcgctgc cccaccagga atggaggccg tttctgcagg
     2101 ggcaatgact gccccatgag gaatgaccgt catttctccc ggggcaacgg ttgccccgtg
     2161 aggaatggcc ctcatttccc tgggaacgaa ttcaaggctg acgacgtact agaatggggt
     2221 ctgattcaag ccaaccggct gccgagggtt tctcatgtca ctacgcggtt tcctgctcgt
     2281 cgctcttctc accggtcccc tttccttctc cgggagcgcc ttcgcgcaga ggagtgaggc
     2341 ccgcgacggc gcgcgcgagc tggccctggg gagggaggcc tatgcacggg ggagctatcc
     2401 cgaggcggtg gggcatctgt cccaggcggt ccaggcgctg ggtgggggag gggagcggga
     2461 ggcgctggcc gatgcctacc tgcggctcgg gatggcgcat ctgatcggct tgggcgcccc
     2521 ggcccaggcg ctgccggcgt tcctcagcag cgccgagctg gccggggagc cggcgaccgc
     2581 ctatctctgg gcggcgctgg cggccgagaa gctcggccag agagaggtct cggagcggct
     2641 tgaagccaag gccctggctg ccgcggttcc agtccctgcc ccggctccgg ttcagacccc
     2701 tgccgcggcg ccggcagccc cgcgggagga gccggcgcga gcccccgagg tgaaggaaac
     2761 ggaagcggag aaggtcgacg ccttccagta tttcttcggc aagaagaagc agaagccgcc
     2821 tgccgagaag cctccgggac tctgaagccg gccgctaccc tccggcgctc cacgtttcca
     2881 gcaggcgctg gagctgctcg ggctctacgg gcttgaccag atgcagatcg aagcccgcct
     2941 cctcggcacg ccggcggtcg gaatcgcggc cgtagccggt cagggcgacc agacggcagc
     3001 gcgtcgtgtc cggatcgctc cggagctgct cggcgacggc gtagccgtcc atcccgggga
     3061 ggccgatgtc gcagagcacc acttccggat ggaagccgcg cgccgcctcg atgccgtccg
     3121 ggccggtgtg ggcgagctcc acctcgtgcc cgaagatcct cagcagatcc cccagcgtct
     3181 ccgcggcgtc ccggttgtcc tcgaccacca gcacgcggag cggcctgccg gcg
//
LOCUS       DASPJI010000538         3808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_435194, whole genome shotgun sequence.
ACCESSION   DASPJI010000538 DASPJI010000000
VERSION     DASPJI010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435194"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /gene="tilS"
                     /locus_tag="VEL74_14730"
     CDS             complement(<1..1087)
                     /gene="tilS"
                     /locus_tag="VEL74_14730"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HYG63833.1"
                     /translation="MDLPSILAAFFRREAPLAPADGLVVAFSGGPDSTALLWGLSRLA
                     AGGSLPLTAAHLDHGMDPGSAGRAAAAARLAHRLRIPFVMERREVPALRRPAESAEAA
                     ARRVRYEFLEEVRARAGARWIATAHHRDDQAETVLLRLLFGSGLAGVAGIRPVHGRVV
                     RPLLEVSRAELAAALSAGSAGSAGSAAGLSPVEDPTNLDPRTPRNRVRHGLLPALEAA
                     EGELAPCLARLAERARGAAAVLESRLAPLLGAETLRGDGGGVAVERGALERLPAELLP
                     FAFAALHRQAGAPYPAGGAARSEVLRQLAGRTRAGCDCGGGWRWEQERERLILRRTAP
                     HSGRTGERVTDFTYTLEVPGELDIPEIA"
     gene            complement(1100..1175)
                     /locus_tag="VEL74_14735"
     tRNA            complement(1100..1175)
                     /locus_tag="VEL74_14735"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1140..1142),aa:Met,seq:cat)
     gene            complement(1369..1695)
                     /locus_tag="VEL74_14740"
     CDS             complement(1369..1695)
                     /locus_tag="VEL74_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYG63834.1"
                     /translation="MANIDRIIPNYRRDERHLTKVFKALSDSTRQEILRLLETEQKTV
                     GQIVGNFNLSQPTISRHLSVLKEADLVVDQRQGQNVIYRLNDSALSTSMQEFFGQFRS
                     CQEVMR"
     gene            complement(1985..2593)
                     /locus_tag="VEL74_14745"
     CDS             complement(1985..2593)
                     /locus_tag="VEL74_14745"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HYG63835.1"
                     /translation="MHELPPLPYSFDALEPHIDAKTMEIHHDKHHATYVSKLNDALKD
                     QPDLQNKPVEELLRDFNSVPQSIQGAVRNHGGGHANHTLFWEIMGPGGGGNPSGDLAS
                     ALDQAFGGFDSFKEKLTNAAANQFGSGWGWLVVDGGKLDVIARPNQDSPLMEGKTPIL
                     GVDVWEHAYYLKYQNKRPDYLAAWWNTINWRAVEDRFRRARG"
     gene            complement(2796..2951)
                     /gene="rpmG"
                     /locus_tag="VEL74_14750"
     CDS             complement(2796..2951)
                     /gene="rpmG"
                     /locus_tag="VEL74_14750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HYG63836.1"
                     /translation="MRDKIKLVSTAGTGYFYTTDKNKKLSTEKLRLKKYDPKVRKHVE
                     FVEEKLR"
     gene            complement(3025..3101)
                     /locus_tag="VEL74_14755"
     tRNA            complement(3025..3101)
                     /locus_tag="VEL74_14755"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3065..3067),aa:Asp,seq:gtc)
     gene            complement(3148..3223)
                     /locus_tag="VEL74_14760"
     tRNA            complement(3148..3223)
                     /locus_tag="VEL74_14760"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3188..3190),aa:Val,seq:tac)
     gene            complement(3349..3543)
                     /locus_tag="VEL74_14765"
     CDS             complement(3349..3543)
                     /locus_tag="VEL74_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63837.1"
                     /translation="MKKSVKKLALHRETVLQLGAMAKVAGGTAQQDMEGTSYGFTEPT
                     GMTAFPCDLFDTWPSCKGLC"
BASE COUNT          616 a         1203 c         1362 g          627 t
ORIGIN      
        1 cggcgatctc cgggatgtcc agctcccccg gaacctccag agtataagtg aaatccgtca
       61 ccctctcgcc ggttcgcccc gagtggggtg ccgtccgccg caagatgagg cgttcgcgct
      121 cctgctccca gcgccagccg ccgccgcaat cgcagcccgc gcgcgtcctg ccggcgagct
      181 ggcgcagcac ctcggagcgg gccgccccgc cggcggggta gggggctccg gcctgccgat
      241 ggagggcggc gaaggcgaag gggaggagct cggccggcag ccgctccagg gcgccgcgct
      301 cgacggcgac cccgccgccg tcccccctca gggtctcggc gccgagcagc ggggcgaggc
      361 ggctctccag gacggcggcg gcgccgcgcg cccgctccgc cagccgggcg aggcagggag
      421 ccagctcgcc ttcggcggcc tccagggcgg ggaggaggcc gtggcggacg cggttgcgcg
      481 gcgtccgcgg gtcgaggttg gtcggatcct ccacggggct gagcccggcg gcgctgccgg
      541 cagacccggc agacccggca gacaacgcgg cggccagctc ggcgcggctc acttccagga
      601 gcgggcgcac cacccggccg tgcaccgggc ggatccccgc gaccccggcg aggccgctgc
      661 cgaacagcag ccgcagcagc accgtctccg cctggtcgtc ccggtggtgg gcggtggcga
      721 tccagcgggc tccggcccgg gcgcgcacct cctccaggaa ttcgtagcgc acccgccggg
      781 ccgccgcctc ggcgctctcg gccggccggc gcagcgcggg gacctcccgg cgctccatca
      841 cgaaggggat gcgcagtcgg tgggcgaggc gcgccgccgc cgccgcccgt ccggccgagc
      901 cggggtccat gccgtggtcg agatgggcag cggtcagcgg caaggagccg ccggcggcca
      961 gccggctcag cccccagagc agggcggtgg agtccgggcc gccggaaaag gccaccacga
     1021 ggccgtctgc aggggcaagg ggtgcctcac gccggaagaa ggcggcgagg atggatggga
     1081 gatccatgaa ggagaaggat ggtggcggtg aagggactcg aacccctgac gcagcggata
     1141 tgagccgctt gctctaccaa ctgagctaca ccgccgggaa aacacgtgga gggtccggag
     1201 gagccctcgg ccgcgggcag gattatactc gccgaagggg ccaacgcaat gtgggcgtcg
     1261 attcattccg cgcagagcct tcccagcggg gagatctctg ccggagggcg agggacgggg
     1321 cgccatccat cgccctccga gggcgcgagg ccgaaatgga ttcggcctct agcgcatcac
     1381 ttcctggcag ctccggaact ggccgaagaa ctcctgcatc gaagtcgaaa gcgccgagtc
     1441 gttcagccgg tagatgacgt tctggccctg acgctggtcg acgaccaggt cggcctcctt
     1501 gaggaccgag agatgacgcg agatggtcgg ctgggacaga ttgaaatttc ccacgatctg
     1561 gccgacggtc ttctgctcgg tctccagcag ccgcaggatc tcctggcggg tgctgtccga
     1621 cagcgccttg aagaccttgg tcaggtgacg ctcatcacgc ctgtaattgg ggatgatccg
     1681 gtcgatgttc gccatgggtt ttctcctcgc caatttggaa tacgccgttg gacctcactt
     1741 ccgatgaacg cattgtagag gaagcatact tgaaacagac ttgatgttgc gctcggtttg
     1801 gggcaaggcg ttgccaaatt tttgagcgca cctgtgcaaa cttctctata acccaaattt
     1861 agctcgatct cggaacggtg ttcgggagag ggaaccgagg gctccgggcg gcccggaacc
     1921 ctcgaaaagg ggtgtcccac cgggggaaca caggggggag aggccctacc tgctcacttc
     1981 gtggtcagcc gcgggcgcgg cggaaccggt cctcgacggc gcgccagttg atggtgttcc
     2041 accaggcggc caggtagtcg gggcgcttgt tctggtactt gaggtagtag gcgtgctccc
     2101 agacgtcgac gcccaggatg ggggtcttgc cctccatcag cgggctgtcc tggttgggcc
     2161 gcgcgatgac gtcgagcttg ccgccgtcca ccaccagcca gccccagccg gagccgaact
     2221 ggttggcggc ggcgttggtg agcttctcct tgaagctgtc gaagccaccg aaggcctggt
     2281 cgagggcgga ggcgaggtcg cccgaggggt tgccgccgcc gccgggtccc atgatctccc
     2341 agaacagcgt gtgattggcg tggccgccgc cgtggttgcg gacggcgccc tggatgctct
     2401 ggggcacgct gttgaagtcg cgcagcagct cctcgaccgg cttgttctgg aggtccggct
     2461 ggtccttgag cgcgtcgttg agcttgctca cataggtggc gtggtgcttg tcatggtgga
     2521 tctccatcgt cttcgcgtcg atgtggggct cgagcgcgtc gaaggaatag ggaagaggcg
     2581 gaagctcatg catcggtgat tccctcctag ctgcgggttg aaggatgggg cggaccgggc
     2641 ggccactctg cggccctcgc cgccgtcccg ggctttttat tctggcatga atgatcgcgc
     2701 cccggggaag gggggcgtcg ggtgccgcgc tgaaggtctg gagaagagga gagggaagcg
     2761 aggagcccgg ggggagcccc cgggccggag ccggctcagc ggagcttctc ttcgacgaac
     2821 tcgacgtgct tgcggacctt ggggtcgtac ttcttcagcc gcagcttctc ggtggagagc
     2881 ttcttgttct tgtcggtcgt gtagaagtag ccggtgccgg cggtcgagac cagtttgatc
     2941 ttgtcgcgca ttgtcttcag tccctctgca gtctccgggt gctgctgatt cccggaacca
     3001 ccgggaagga atttcagatg taaatggcgg ggccgacggg actcgaaccc gcgacctccg
     3061 gcgtgacagg ccggcattct aaccgactga actacgaccc cgcaaagatc tttcctacga
     3121 gacggcgccc caccttctac atcgagatgg tgggaggtga gggatttgaa cccgcgaccc
     3181 cctgcgtgta aggcaggtgc tctaaccgct gagctaacct ccctccaagc cgcccgagga
     3241 ggccagatac tacgcaagcc gccccggcgt gtcaacaggg ggcgtcaaga aaaattctcg
     3301 acgcccccgg attcggctca acgccgacct ggctccttgg ctagcctact agcagagacc
     3361 tttgcacgag ggccaggtgt cgaaaagatc gcagggaaag gcggtcattc cagtcggctc
     3421 ggtaaaccca tagctggtgc cctccatatc ttgctgggca gtgccacccg cgaccttcgc
     3481 catggcgccg agttggagga cggtctcacg gtggagggcg agcttcttga ctgacttctt
     3541 caagctggtc tccttgtcga gaacgggtgg agggaggact ctagggggcg tcaggcgagc
     3601 tgtcaagcga gtcgagagag cggcccacgg aggaaccgct ccctcggacc ggtctcctgc
     3661 cgttcctagc agaaggtggt gtagacgggg atgtcgcagg ggaaggcggt gatcccaccc
     3721 ggctgcgtga acgtgatgtc gcgctccttg tagcgggtcg cccaggtgcc ggcggcgacc
     3781 ttcgccatag cgtcgagccg gacgacgg
//
LOCUS       DASPJI010000539         3288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_780986, whole genome shotgun sequence.
ACCESSION   DASPJI010000539 DASPJI010000000
VERSION     DASPJI010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780986"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..835
                     /locus_tag="VEL74_14770"
     CDS             227..835
                     /locus_tag="VEL74_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63838.1"
                     /translation="MAYEGAGKIFLLSPASASGVRASQLTSPRAGFSTARQFRSPEGV
                     PIEEAFTFMSSLYFRGKITYARHFAAPPDGFPGDGILVIAPGFGLVPPEWPLTIERMR
                     RLSRTPVDLKSRSYCLPMRGHAQQLADNLPPDARVILLGSIATGKYVDLLLPVFGDRL
                     LFPRAFVGAGDMKRGGLMLRAVREDTELDYSTLDIPRRASRS"
     gene            976..1236
                     /locus_tag="VEL74_14775"
     CDS             976..1236
                     /locus_tag="VEL74_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63839.1"
                     /translation="MLSKNSALKTLALGSALVATIATTGCRVQQTEEGEMPDVDVSAE
                     GGKMPEYDVDAADVEVKTEERTIKVPDVDVKPPAEDSDPPRQ"
     gene            complement(1325..1765)
                     /locus_tag="VEL74_14780"
     CDS             complement(1325..1765)
                     /locus_tag="VEL74_14780"
                     /inference="COORDINATES: protein motif:HMM:NF040124.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6526 family protein"
                     /protein_id="HYG63840.1"
                     /translation="MEQSYRNHRQFVPLYHFVTGSILLLNLIWALYRLVRGLPDVPLF
                     DRVLGVLVALALFSIYFYARIFALRVQDRVIRLEMRLRLARLLPPDLQPRIGELSTGQ
                     LIGLRFASDEELPELTRKVLDERITKREAIKKMVRRWEADHLRL"
     gene            1836..>3288
                     /locus_tag="VEL74_14785"
     CDS             1836..>3288
                     /locus_tag="VEL74_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HYG63841.1"
                     /translation="MHDSTAYPWDRFVSSFLESYFEAQPVFAAGAGRHEFDGRLPDWS
                     PEALAGEAERLRTERRRAEAFDPADLDEPRRFEREYVLAQIDCDLFWLVEADSPHRNP
                     LFYAGALDPSLYTTRPYAPLAERLAAFIRYARQVPRAAAQVRANLRSPMPRTFAELGA
                     MTFSGLADYYENDVPVVFAGVEDERLLAEMRDASAPAAAAMRELGAWFQEQSAAPGSD
                     GFRLGPDLYRRMLRETERVDVPLAELEAAGRRELERNLGALREACAAYAPGLPVAECL
                     ARVQAAKPAGGPVEGARRQLAGLRRFVEEQELVSIPGDEEALIEEAPPYQRWNSAYID
                     IPGPYDSHLPSIYYIAPPDPAWSEEERAAYIPGEADLLFITAHEVWPGHFLQFLHAHR
                     VASPIGRLFVGYGFAEGWAHYSEELMWETGFGAGDPAVQIGQLQNALLRNVRYLVALG
                     LHTGDLTLEEAERMFREQAFQDAASARQQAARGT"
BASE COUNT          516 a         1142 c         1128 g          502 t
ORIGIN      
        1 ggtccgggaa gggacggccg ggggccgcgc cgacgccgga ggtgatggag tcgcccaggc
       61 agacgatcgt cgtgcccggt gaatccaggt tcgggatgtc cgagtccggg ccgcaggcgg
      121 ccagaaggag cagggacgcg aggcacgagg cggtggcgcg tttcacggcg cgcattctag
      181 cagtggcggg aatgcacgac ggggccggcc tgttctgcca ggacatatgg cgtacgaggg
      241 tgccggcaag atcttcctcc tctcccccgc gagcgcttcg ggcgtccggg cgagccagct
      301 cacctcgccg cgggcggggt tctccaccgc ccggcagttc cgcagccccg agggtgtgcc
      361 catcgaggag gcgttcacct tcatgagctc cctctatttc cgcggcaaga tcacctatgc
      421 ccgccatttc gccgcgccgc ccgacggctt ccccggtgac ggcatcctgg tgatcgcacc
      481 gggcttcggc ctggtaccgc cggagtggcc gctcaccatc gagcggatgc ggaggctctc
      541 gcgcaccccc gtggacctca agagccgttc ctattgcctc cccatgcgcg gccacgccca
      601 gcagctcgcc gacaacctcc cgccggatgc ccgggtcatc ctcctgggaa gcatcgccac
      661 gggcaagtac gtggacctcc tcctcccggt tttcggcgat cgtctgctct tcccgcgcgc
      721 gtttgtcgga gccggcgaca tgaagcgcgg cggcctcatg ctgcgcgcgg tgcgggaaga
      781 taccgagctc gactactcca ccctcgacat cccacgccgc gccagccgct cctgagccag
      841 gggaggcagg agctggtacg gcccttgcaa tttacggtag gcaaggacgg cccccagagt
      901 tgaagggtcg acgagctgaa cgaaagaaac ggatattccg aaatcagtag cagagacaaa
      961 agaggaggaa acaccatgct ttccaagaat tctgctctca agacgttggc cctcggctcg
     1021 gcgctggtgg ccaccatcgc gaccaccggc tgccgcgtgc agcagaccga agagggtgag
     1081 atgcccgatg tcgacgtgag cgccgagggc ggcaagatgc ccgagtacga cgtcgacgcc
     1141 gctgatgtcg aggtgaagac cgaggagcgg acgatcaagg tccccgacgt cgacgtgaag
     1201 ccgccggcgg aggattccga tccgccgcgt cagtaagaaa ccttactgac accctgagat
     1261 cccaaggcgg cgggcccagc tcgccgcctc tcgtcatttt tctcctgaaa acctttcttt
     1321 gtcgtcagag ccgcaggtga tcggcctccc agcgccgcac catcttcttg atcgcttcgc
     1381 gtttcgtgat ccgttcgtcc agcaccttgc gggtgagctc ggggagctct tcgtccgagg
     1441 cgaagcgcag gccgatgagc tgtccggtcg acagctcgcc gatgcgcggc tggaggtccg
     1501 gcggcagcag gcgcgcgagg cgcaggcgca tctccagacg gattacccgg tcctgcaccc
     1561 tgagagcgaa gatccgggca tagaaataga tggagaacag ggcgagcgcc acgagcaccc
     1621 ccagcacccg gtcgaacagc ggcacgtccg gcagcccccg gacgagccgg tagagcgccc
     1681 agatgaggtt cagcagcagg atgctccccg tgacgaaatg gtagagcggc acgaactggc
     1741 ggtgattccg gtagctctgc tccatggttt ctcccgagac gattttcggc ggatggtatc
     1801 gcatcgcggc cctccgccgg gctagactgc gcgccatgca cgactccacc gcgtacccct
     1861 gggaccgctt cgtctcctcc ttcctggaga gctacttcga ggcgcagccg gtcttcgccg
     1921 ccggcgccgg ccgtcacgag ttcgacggcc gcctcccgga ctggagcccg gaggcgctgg
     1981 ccggcgaggc ggagcgcctg cggaccgagc ggcggcgggc cgaggccttt gatccggcgg
     2041 acctggacga gccccggcgc ttcgagcgcg aatacgtgct ggcccagatc gactgcgacc
     2101 tcttctggct ggtggaggcc gactcccccc atcgcaaccc gctgttctac gccggcgccc
     2161 tggaccccag cctctatacg acacgtccct acgcgccatt ggcggagcgc ctcgcggcct
     2221 tcatccgcta cgcgcgccag gtgccgcggg cggcggctca ggtgcgggcg aacctgcgga
     2281 gccccatgcc gcgcaccttc gccgagctcg gcgcgatgac cttctccggc ctcgccgact
     2341 actacgagaa cgatgtcccc gtcgtcttcg caggtgtcga ggacgagagg ctgctggccg
     2401 agatgcggga tgccagcgcc ccggcggcgg cggcgatgcg ggagctgggc gcctggttcc
     2461 aggagcagtc cgccgctccg ggcagcgacg gcttccggct cggccccgac ctctaccggc
     2521 ggatgctgcg cgagaccgag cgggtggacg tgccgctcgc cgagcttgag gcggccggcc
     2581 ggagggagct ggagcggaac ctgggagcgt tgcgggaggc ctgcgccgcc tacgcgccgg
     2641 ggctcccggt ggcggagtgc ctcgcccggg tgcaggcggc gaagcccgcc ggcggtccgg
     2701 tggagggggc gcgccggcag ctcgccgggc tccgccgctt cgtcgaggag caggagctgg
     2761 tcagcatccc cggcgacgag gaggcgctga tcgaggaggc gcccccctat cagagatgga
     2821 actcggccta catcgacatc ccggggccgt acgacagcca tctgccgtcc atctactaca
     2881 tcgcgccgcc ggaccccgcc tggtccgagg aggagcgggc cgcctacatc ccgggcgagg
     2941 ccgatctcct cttcatcacg gcgcacgagg tctggccggg gcactttttg cagttcctgc
     3001 acgcccaccg ggtggcctca ccgatcggcc ggcttttcgt cggctacggc ttcgccgagg
     3061 ggtgggccca ctactcggag gagctgatgt gggagaccgg cttcggcgcg ggcgaccccg
     3121 ccgtccagat cgggcagctc cagaacgctc tcctccgcaa tgtccgctac ctggtggctc
     3181 tcgggctcca caccggcgat ctaacgctgg aggaggcgga gcgaatgttc cgcgagcagg
     3241 ccttccagga cgcggccagc gcccgccagc aggcggcccg gggcacct
//
LOCUS       DASPJI010000540         9082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_781002, whole genome shotgun sequence.
ACCESSION   DASPJI010000540 DASPJI010000000
VERSION     DASPJI010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9082)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9082
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781002"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1331)
                     /locus_tag="VEL74_14790"
     CDS             complement(192..1331)
                     /locus_tag="VEL74_14790"
                     /inference="COORDINATES: protein motif:HMM:NF024971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HYG63842.1"
                     /translation="MNRHRFALSCLIAGALLGTPAAHAAQPSTGTVAPPNVSATWTGG
                     PYTLGVPNQLGCLFPGNPTCDAFNLTVNLPVNYRFAVAITSEIEGDDYDLYVYYPDGT
                     EAARSANDGGNEQVVIKHTTAHGTGPYKVRVLPFSVTSGSSYAGVARATSAQTIEPAD
                     PNACLEAVPAAVGIPGITDLGQDVSLDVAVLLDGVSVGEADQIMSKAAISYAPLKVDL
                     NVVSYTPVSFTGTEGSALIQQAKDLFGGVRPAGSDLVYILSNKDLTTDGDTALAGLAD
                     CIGGVRFPQHAFAVGEAVGIEPSAIGSFLLQVDAHPEVAAHEIGHLMGVHHHYANCVE
                     GILQGEANDLSPCTLMFNFVDFQSLNFGVLEGIVARGHAVQYASP"
     gene            complement(1449..2174)
                     /locus_tag="VEL74_14795"
     CDS             complement(1449..2174)
                     /locus_tag="VEL74_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYG63843.1"
                     /translation="MSELSGKVALVTGATGGIGAAISLALANEGMRLFLTGRDNGRLE
                     DLSLRTLKHALSVEVHRADLEDDAQLRAMAAHAEELFGGVDVLVHAIGLFRGGDLEHA
                     PVEDLDRQYRVNVRVPYLLTQTLLPSLTARQGQVVFVNSSAGHHPGRAGFGGYAATKH
                     GLSALADSLREEVNRRGVRVLTVFPGRTATSMQAEVHRFEGKTYEPERFLQPDDVASA
                     VVHALSLPRTAELTDLHLRPMRP"
     gene            complement(2171..3523)
                     /locus_tag="VEL74_14800"
     CDS             complement(2171..3523)
                     /locus_tag="VEL74_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HYG63844.1"
                     /translation="MPTLSTGPTGVGERLRGFDAQALGAEMHGFIAEAYPICRSITGD
                     GVRQTLRMVGERIPLQVHEVPTGTPAFDWTVPKEWNIRDAWIKDPRGERVVDFRRSNL
                     HVLNYSAPVHEKLSLDALRPHLFTLPEQPDRIPYRTSYYRETWGFCLSHHQLLSLQEG
                     EYEVCIDSTLADGSLTYGECFLPGETEGEVLISCHVCHPSLANDNLSGIAVASFLARE
                     LAGLRLRYSYRFVFIPGTIGSIVWLSRNEERAGRIAHGLVAANLGDPGHFHYKRSRQG
                     DAEIDRAVTRVLRDSGEEHGVAEFIPFGYDERQYCSPGFDLPVGSLTRTPYGRYSEYH
                     TSGDNLDFVRPEALAGSLRTYLSVVDVLEGNGRFLNLNPKCEPQLGRRGLYRTLGGDE
                     KGREREMALLWVLNLSDGRHTLLDIADRSGMSFAAVREVCEALLEVELLRELEPEASS
                     "
     gene            complement(3523..4053)
                     /gene="rfbC"
                     /locus_tag="VEL74_14805"
     CDS             complement(3523..4053)
                     /gene="rfbC"
                     /locus_tag="VEL74_14805"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HYG63845.1"
                     /translation="MIFRETDLAGAWVLEPERFTDDRGFFARTFCRDELQAHGIDPSV
                     AQCSVSYNHRKGTLRGLHFQIAPREEAKLVRCTRGAIFDAIVDVRPGSPTYGRHFAVV
                     LSAEAGNQLYIPKGMAHGFQTLEDGTEIFYQISEMYAPELARGYRYDDPALGIPWPLP
                     VTVIADKDLDLPRFEA"
     gene            complement(4050..5279)
                     /locus_tag="VEL74_14810"
     CDS             complement(4050..5279)
                     /locus_tag="VEL74_14810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG63846.1"
                     /translation="MTPACRFCGRPLALTFADLGVQPLANSYVEPADLQKAETFYPLH
                     VYVCEGCWLVQLPEAASPETIFSDYAYFSSYSESWLRHAQRYAEAMIPRFGLGPDSLV
                     VEIASNDGYLLRFFKERGVPVLGVEPAGNVAKAAEELGIPTVVRFFGTELARELAAGE
                     RRADLLVGNNVLAHVPDLNDFVQGLATLLAPGGVLTMEFPHLLRLMEEDQFDTIYHEH
                     FSYFSLSAVRRVFAHHGLTVVDVEELPTHGGSLRVFARCSDGAGQPTERATELLAREQ
                     AAGLETSGPYQAFAERVRRVKRQLLRFLVEAKEEGRTIVGYGAPAKGNTLLNYCGIGP
                     DFLDYTVDRSPHKQGRFLPGTRVPILGPDRIRETRPDYVLILPWNLKDEIMEQMADVR
                     SWAGKFVVAIPEVRVLS"
     gene            complement(5276..6073)
                     /gene="rfbF"
                     /locus_tag="VEL74_14815"
     CDS             complement(5276..6073)
                     /gene="rfbF"
                     /locus_tag="VEL74_14815"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HYG63847.1"
                     /translation="MTRPAKRDMPVVILCGGQGTRLREETEYRPKPMVEIGERPILWH
                     IMKTYSVFGLDRFVLCLGYKGWEIKQYFLRYKEMLGDFTLSMDGSRPLEFHNDLGSES
                     WRVTCAETGLDTATGARLRRVRQYVDAPTFCFTYGDAVGNVDIDALLDFHWSQGRIAT
                     VTGVRPTSRYGEMEVDGTRVREFAEKPVASGVVSGGFFVFQREVFDYLDDDSQLFLEQ
                     QPLQKLARDGQLSVHLHEGYWHPMDTYRDYLHLNAVWKQENPPWKIW"
     gene            complement(6104..7894)
                     /locus_tag="VEL74_14820"
     CDS             complement(6104..7894)
                     /locus_tag="VEL74_14820"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYG63848.1"
                     /translation="MKPLRSVCLGGFLYGAAFYGMVLSLVHVLYNQLGSTRDALVTFV
                     VFILLYGVWAMVFFAGAHVAVRILSRVRRRDGGETGRISLWPGLFVFNSVFWTAFFLY
                     GITYEEVPIGHPSGPAGMLGYVALVALLVALGSALVSWLLARLFARLGRRLVPVAVSA
                     LLLGLVIHAAAPLYTGRPEVEEEKAAAEAPKIQGAETGLKVVLVGFDGADWRVIRPLI
                     DKGQLPTFAGMVRHGATGPLATIHDSNSAVIWASIYSGTEPVRHGIHDFYRVELPGMA
                     SAGLFPVHRSYFKELADLAGKVGLAHQTLVTRFSQRTVPIWEIVDHAGLSLGLVDGYL
                     YSVPALKPSHPESFFLSYGLDGFAQLPGGKSLEDAEVFIQPARLFRQVRPYLDRDDFY
                     WQSAALFDLLGRYPQPRFLNFYTHEPDSFQHWYWKWLQPEYFLRVTKEGMDENGDRIP
                     KLHRDFDAFLKRLMATVGPDTVVIVASDHGHAPTILHGNFYSQHRHGPPGILLMQGGP
                     VRPGVSLQGKDIYDVFPTVLYLLGLPVPKDIPGKPMLDALDPEFVRRHPLRSIDSYEA
                     LGLAKGLSGAGRGEELNRQEIEKLKSLGYL"
     gene            complement(8108..8812)
                     /locus_tag="VEL74_14825"
     CDS             complement(8108..8812)
                     /locus_tag="VEL74_14825"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYG63849.1"
                     /translation="MVRLFCFPYGGSGAMGYRRWAKSLPPGIELVPVQIPGRESRFSE
                     KPYEHAEPLARDLADRLDTGRPYAFFGHSFGALAAFELARELRRRGRPLPEALLLSGL
                     PAPHLARRRPRIAHLSDAEFIATLRDQFDLTDELVANHDLMEMVLPIMRADFAVVENY
                     VCPEEPPFEIPIRVFGGLSDPEVTREELDAWQKHSARPVAVRVFLGGHFFINTNQEFL
                     PALSTALRETLGARIG"
     gene            complement(8884..>9082)
                     /locus_tag="VEL74_14830"
     CDS             complement(8884..>9082)
                     /locus_tag="VEL74_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HYG63850.1"
                     /translation="NRYGMTAEQTAFFDFFSGLPPTFEPEALAVIAAGLDKGAEPREI
                     KRSARLLQAYEKDFWEAVGRR"
BASE COUNT         1504 a         3019 c         3241 g         1318 t
ORIGIN      
        1 gctggcggag ataccacgcg cttcacggcc gagacctgcg atctcaacaa cgaagtctgc
       61 gcgaaccacg atgacccctc gatcggctcg ctctgtattt ccatcggctg ctgaggggcc
      121 aaggcggctc tgaagcgcgc acggcggctc cgaaaggagc cgccgtgcgt gggccatcgc
      181 cgggcgggag gctacggcga cgcgtactgg acggcgtgac cgcgggcgac gatcccttcg
      241 agcacgccga agttcagcga ctggaagtcg acgaagttga acatcagcgt gcagggggac
      301 aggtcgttcg cctcgccctg gaggatcccc tcgacgcagt tggcgtagtg gtggtggaca
      361 cccatcagat ggccgatctc atgcgcggcg acctccgggt gagcgtcgac ctgcaggagg
      421 aaggagccga tggcggacgg ctcgatcccc accgcctcgc cgaccgcgaa ggcgtgctgc
      481 gggaaccgca cgccgccgat gcagtcggcc aggccggcga gcgcggtgtc gccgtccgtc
      541 gtgaggtcct tgttgctgag gatgtagacc aggtccgagc cggcaggccg gacgccgccg
      601 aagagatcct tggcctgctg gatcagcgcg gagccctcgg tgcctgtgaa ggacacgggg
      661 gtatacgaga cgacgttcag gtcgaccttg agcggcgcgt acgagatcgc ggccttgctc
      721 atgatctgat ccgcctcacc gacgctgacg ccgtccagga ggaccgccac gtccagggac
      781 acgtcctgcc cgaggtccgt gatcccgggg atgccgaccg ccgcgggcac cgcctccaga
      841 caggcgttcg ggtccgccgg ctcgatggtc tgtgcgctgg tcgcgcgcgc gacgccagcg
      901 taggaggagc cggaggtgac cgagaacggc agcacccgca ctttgtaggg gccggtgcca
      961 tgggcggtgg tgtgcttgat caccacctgc tcgttgccgc cgtcattggc cgagcgggcg
     1021 gcctcggtgc catcgggata gtagacgtag aggtcatagt cgtcgccctc gatctcgctg
     1081 gtgatcgcga cggcgaagcg atagttcacc ggcaggttga cggtgaggtt gaaggcgtcg
     1141 caggtggggt tgccggggaa gagacacccc agctggttcg ggactccgag ggtgtagggc
     1201 ccgccggtcc aggtggcgga cacgttgggc ggggctaccg tgcccgtgct gggctgtgcg
     1261 gcgtgggctg cgggggtgcc gagcagagct ccggcaatca gacaactcag agcaaaacga
     1321 tggcggttca tgaaatgatc ctcctctcct aacgaatttg ggcgctttgc aagggtagtg
     1381 ccactactga gcggcagctc cttttatcag tccggtgcgc gaggaggacg gggacgagcc
     1441 gtgcccagtc agggacgcat gggacggagg tgcagatcgg tgagctcggc ggtacgcggc
     1501 agcgacaggg cgtggaccac ggccgaggcg acatcatccg gctgcaggaa gcgctccggc
     1561 tcgtaggtct tgccttcgaa gcggtgcacc tcggcctgca tggaggtggc ggtccgcccc
     1621 gggaagacgg tgaggacccg caccccccgg cggttgacct cctcccgcag gctgtcggcc
     1681 agcgcgctca ggccgtgctt ggtggccgcg tagccgccga agccggcccg cccggggtga
     1741 tgcccggcgc tggagttgac gaaaacgacc tgcccctggc gcgccgtcag ggaggggagg
     1801 agagtctggg tgaggaggta ggggacgcgc acgttcaccc ggtattggcg gtccaggtcc
     1861 tccaccggcg cgtgctccag gtccccgccc cggaacaggc cgatggcgtg gaccaggacg
     1921 tcgactcccc cgaagagctc ctccgcgtgg gcggccatgg cccggagctg cgcgtcgtcc
     1981 tcgaggtcgg cgcgatggac ctccaccgaa agcgcgtgct tcagcgtcct gagggacaga
     2041 tcctccagcc gcccgttgtc gcggccggtg aggaagagcc gcatcccctc gttggcaagg
     2101 gcgaggctga tggcggcacc gatgcccccg gtcgccccgg tcaccagggc gaccttgccg
     2161 ctcagctcgc tcacgacgac gcctccggct ccagctcgcg cagcagctcc acctccagca
     2221 gggcctcgca gacctcccgc acggcggcga aggacatccc ggaccggtcg gcgatgtcca
     2281 gcagggtgtg ccgcccatcc gatagattga gcacccagag cagggccatc tccctctccc
     2341 tccccttctc gtcgccgccc agggtccggt agagcccccg ccgcccgagc tgcggctcgc
     2401 acttgggatt caggttgagg aaacggccgt tcccctccag gacgtccacc accgagaggt
     2461 aggtgcggag cgatccggcg agggcctccg gccgcacgaa gtccaggttg tcccccgagg
     2521 tgtggtactc ggagtagcgg ccgtaggggg tacgggtgag cgagccgacg ggcaggtcga
     2581 acccgggcga gcagtattgc cgctcgtcgt agccgaaggg gatgaactcg gcgacgccat
     2641 gctcctcccc cgagtcccgc agcaccctcg tcaccgcgcg gtcgatctca gcatcgccct
     2701 gccggctccg cttgtaatgg aagtgccccg ggtcccccaa gttggccgcc accaggccgt
     2761 gggcgatccg ccccgcccgc tcctcgttgc gcgacagcca gacgatggag ccgatggtgc
     2821 cggggatgaa gacgaaccgg tacgagtagc gcagccgcag gcccgccagc tcccgcgcca
     2881 ggaaggaggc gaccgcgatc cccgacaggt tgtcgttggc gagcgacgga tggcagacgt
     2941 ggcaggagat caggacctcg ccctccgtct cccccggcag gaagcactcg ccgtaggtca
     3001 gcgacccgtc ggccagggtg gagtcgatac agacctcgta ctccccctcc tggagggaga
     3061 ggagctggtg gtggctcaga cagaagcccc aggtctcccg gtaataggag gtgcggtagg
     3121 ggatgcggtc cggctgctcg ggaagcgtga agaggtgcgg gcgcagcgcg tccagcgaga
     3181 gcttctcgtg caccggcgcg ctgtagttga ggacgtggag gttggagcgc cggaaatcca
     3241 ccaccctctc gccccgcggg tccttgatcc aggcgtcccg gatgttccac tccttgggca
     3301 cggtccagtc gaaggccggc gtgccggtgg ggacctcgtg gacctggagc gggatcctct
     3361 cccccaccat tctcaaagtc tggcggacgc cgtcgccggt gatgctgcgg cagatggggt
     3421 aggcctcggc gatgaagccg tgcatctccg cccccagggc ctgggcgtcg aagccgcgca
     3481 gccgctcgcc gacccctgta ggccctgtag acagcgtggg catcaggcct cgaaccgcgg
     3541 caggtcgagg tccttgtccg cgatcaccgt cacgggcagg ggccagggga tgcctaacgc
     3601 ggggtcgtca taccggtaac cgcgggcgag ctccggcgcg tacatctcgg agatctgata
     3661 gaagatctcg gtcccgtcct ccagcgtctg gaagccgtgc gccatccctt tggggatgta
     3721 gagctggttg cccgcctcgg ccgacagcac cacggcgaag tgccggccgt aggtcggcga
     3781 gccgggacgg acgtcgacga tggcgtcgaa gatggcgccg cgggtgcagc gcaccagctt
     3841 cgcctcctcg cggggcgcga tctggaagtg gagcccgcgt agcgtcccct tgcggtggtt
     3901 gtacgagacg ctgcactgcg ccaccgaggg atcgatgccg tgggcctgga gctcgtcccg
     3961 gcagaaggtg cgggcgaaga acccccggtc gtcggtgaag cgctccggct ccaggaccca
     4021 ggcgccggcg aggtcggtct cccggaagat cacgacagga cccgcacctc ggggatggcg
     4081 accacgaact tcccagccca ggagcggacg tcggccatct gctccatgat ctcgtccttc
     4141 aagttccagg ggaggatcag cacgtagtcg ggccgggtct cccggatgcg gtccgggccg
     4201 aggatgggga cgcgcgtccc gggcaggaac cgcccctgct tgtgcgggct ccggtcgacc
     4261 gtgtagtcga ggaagtcggg gccgatgccg cagtagttga gcagcgtgtt ccccttggcc
     4321 ggcgcgccat agcccacgat cgtccgcccc tcctccttgg cctctaccag gaagcgcagg
     4381 agctggcgct tgacccgccg gacccgctcg gcgaaggcct ggtagggccc ggaggtctcc
     4441 agtcccgcgg cctgctcgcg cgccagcagc tccgtcgccc tctccgtggg ctggccggcg
     4501 ccgtcggagc accgggcgaa gacccgcagc gatcccccat gggtgggcag ctcctcgacg
     4561 tccacgacgg tgaggccgtg gtgggcgaag acccgccgga ccgccgacag cgagaagtag
     4621 gagaagtgct cgtgatagat ggtgtcgaac tggtcttcct ccatgagccg cagcaggtgc
     4681 gggaactcca tggtgaggac gccgccgggc gcgagcaatg tcgcgagacc ctggacgaag
     4741 tcgttcaggt ccggcacatg ggcgaggacg ttgttgccca ccagcaggtc ggcccgcctc
     4801 tccccggcgg ccagctcccg ggccagctcg gtaccgaaga agcggaccac ggtggggatg
     4861 cccagctcct ccgccgcctt ggcgacgttg cccgccggct cgacgccgag caccggcacc
     4921 ccccgctcct tgaagaaacg cagcaggtag ccgtcgttgc tggcgatctc caccaccagg
     4981 ctgtccggcc cgaggccgaa gcgggggatc atcgcctcgg cgtagcgctg ggcgtggcgc
     5041 agccagctct ccgagtagga ggagaaatag gcgtagtcgg agaagatcgt ctccggcgac
     5101 gccgcctccg ggagctggac gagccagcac ccctcgcaga cgtagacgtg gagcggatag
     5161 aacgtctcgg ccttctgcag gtccgccggc tcgacgtagg agttggcgag cggctggacc
     5221 ccgaggtcgg cgaaggtgag ggcgaggggg cggccgcaga agcggcaggc cggggtcacc
     5281 agatcttcca gggcgggttc tcctgcttcc agaccgcgtt caggtgcaga tagtcgcggt
     5341 aggtgtccat ggggtgccag tagccctcgt gcaggtggac cgagagctgg ccgtcgcggg
     5401 cgagcttctg aagcggctgc tgctccagga agagctgcga gtcgtcgtcc aggtagtcga
     5461 agacctcgcg ctggaagacg aagaagccgc cggagaccac gcccgacgcc accggcttct
     5521 cggcgaactc gcggacgcgg gtgccgtcca cctccatctc gccgtagcgg gatgtggggc
     5581 ggacgccggt caccgtggcg atccgcccct gcgaccaatg gaagtcgagc agcgcgtcga
     5641 tgtccacgtt gcccaccgcg tccccgtacg tgaaacagaa ggtgggcgcg tccacgtact
     5701 ggcggacccg gcgcaggcgg gcgccggtgg cggtgtcgag gccggtctcg gcgcaggtga
     5761 cgcgccagct ctcggagccg aggtcgttgt ggaactcgag cgggcggctg ccgtccatgg
     5821 agagggtgaa gtcgcccagc atctccttgt aacgcaggaa gtactgcttg atctcccaac
     5881 ccttgtagcc gaggcagagg acgaagcggt ccagcccgaa gacgctgtag gtcttcatga
     5941 tgtgccagag gatcggccgc tcgccgatct ccaccatggg cttgggccgg tactcggtct
     6001 cctcgcgcag ccgcgtaccc tggccgccgc agaggatcac taccggcatg tcacgcttcg
     6061 cgggcctggt catccgttcc atctcctggc gggattctat cgtctacaga tagcccagcg
     6121 acttgagctt ctcgatctcc tgacggttca gctcctcgcc gcgccccgcg cccgacagcc
     6181 ccttggcaag ccccaacgct tcatagctgt cgatcgagcg caacgggtgc cggcggacga
     6241 actcggggtc cagcgcgtcg agcatcggct tgccggggat gtccttcggc accggcagcc
     6301 ccagcaggta gaggaccgtg ggaaacacgt cgtagatgtc cttcccctgg agagacacac
     6361 ccgggcgcac cggtcccccc tgcatcagga ggatgcccgg ggggccgtgc cggtgctggc
     6421 tatagaagtt gccatgcagg atggtggggg catggccgtg atccgaggcg acgatgacca
     6481 ccgtgtccgg cccgaccgtc gccatcagcc gcttcaggaa cgcgtcgaag tcgcggtgga
     6541 gcttcgggat gcggtcgccg ttctcgtcca tcccctcctt cgtcacccgc aggaagtact
     6601 ccggctggag ccacttccag taccagtgct ggaagctgtc gggctcgtgg gtatagaagt
     6661 tgaggaagcg cggctgcggg tatcgcccca gcagatcgaa cagcgccgcc gactgccagt
     6721 agaagtcgtc ccggtcgagg tagggccgca cctgccggaa gagccgtgcc ggctggatga
     6781 agacctcggc gtcctcgagg ctcttgccgc cggggagctg ggcgaagccg tccaggccgt
     6841 aggagaggaa gaagctctcg gggtgggagg gcttgagcgc cggcacggag tagaggtagc
     6901 cgtccaccaa gccgagggag agcccggcgt ggtcgacgat ctcccagatc ggcacggtgc
     6961 gctgcgagaa ccgggtgacg agggtctggt gcgccagccc gaccttaccc gcgaggtccg
     7021 ccagctcctt gaagtaggag cggtggaccg ggaagagccc tgccgaggcc atgcccggca
     7081 gctcgacgcg atagaagtcg tggatgccgt ggcgcaccgg ctccgtcccc gagtagatgg
     7141 aggcccagat caccgccgag ttggagtcgt ggatggtggc gagcggcccg gtggcgccat
     7201 gacggaccat gccggcgaac gtcgggagct gccccttgtc gatgagcggg cggatcaccc
     7261 gccagtcggc gccgtcgaag ccgacgagga ccaccttgag ccccgtctcc gccccctgga
     7321 tcttcggcgc ctccgcggca gccttctcct cctccacctc gggccgcccg gtatacagcg
     7381 gagccgcggc atggattacc agcccgagca ggagggccga gaccgccacc ggcaccagcc
     7441 gccgccccag ccgggcgaac agccgcgcca gcagccacga gaccagggca gaccccagcg
     7501 ccaccagcaa ggcgacgagc gccacatagc cgagcatgcc cgccggaccc gacgggtgac
     7561 cgatggggac ctcctcatag gtgatcccat agaggaagaa ggccgtccag aacacagaat
     7621 tgaagacgaa gagccccggc cacagggaga tccgcccagt ctctcctcca tcccgccggc
     7681 gcacccggga gaggatccgg acggccacgt gcgcgccggc gaagaacacc atcgcccaca
     7741 ccccgtacag caggatgaag accacgaacg tcaccagcgc atcgcgggtg gagccgagct
     7801 ggttgtagag gacatggacg agggacagga ccatcccgta gaaggccgcg ccatagagga
     7861 accctcccag gcagaccgaa cgtagtggct tcatgatgga gcgagaggct tcacggattc
     7921 tctggaggga ccgtcccgac cgtcccagac gctagcagat cacgccgcgg cgcgacaagg
     7981 ggcgggggcg gccggggttg gggtccgatg gcccggcgca ggcgactggc agcgtggccg
     8041 gggggagggt ggagcgcaca cgaaggagct gagcagggcc atccgacccc agccccggcc
     8101 ggggggctca gccgatccgc gccccgagcg tctcgcgcaa cgccgtggac agcgccggca
     8161 ggaactcctg gttggtgttg atgaagaagt ggccgcccag gaacacccgc acggcgaccg
     8221 gccgggcgct gtgcttctgc caggcgtcca gctcctcccg ggtgacctcg gggtcggaca
     8281 ggccgccgaa cacccggatc ggaatctcga acggcggctc ctccggacag acatagttct
     8341 ccaccaccgc gaagtcggcc cgcatgatcg ggagcaccat ctccatgaga tcgtggttcg
     8401 cgaccagctc atcggtgagg tcgaactggt cgcgcagcgt ggcgatgaac tcggcgtcgg
     8461 agagatgggc gatgcggggg cggcgccgcg ccaggtgggg ggcgggcagc ccggagagca
     8521 ggagcgcctc ggggagcggc ctcccccgcc gccgcagctc gcgcgccagc tcgaaggcgg
     8581 ccagggcccc gaagctgtgg ccgaagaagg cgtacggccg cccggtgtcg agccggtccg
     8641 ccaggtcgcg ggcgagcggc tcggcgtgct cgtacggctt ctccgagaaa cgcgactccc
     8701 ttcccgggat ctgcaccggg acgagctcga taccgggcgg gagggacttc gcccagcgac
     8761 ggtaccccat cgcccccgag ccgccatagg gaaaacagaa cagacgcacc atgtcatcgc
     8821 ctccgcgctc cgggctcccc cggagcgcgg gcgattctac acggctgtga gggcaggggc
     8881 gcctcagcgc cggccgaccg cctcccagaa gtccttctca taagcctgga gcaggcgggc
     8941 cgagcgcttg atctcccgcg gctcggcacc cttgtccaga ccggcggcga tcaccgccag
     9001 ggcctcaggc tcgaacgtcg gggggaggcc ggagaagaag tcgaagaacg cggtttgctc
     9061 ggcggtcatg ccgtagcggt tg
//
LOCUS       DASPJI010000541         3355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_331793, whole genome shotgun sequence.
ACCESSION   DASPJI010000541 DASPJI010000000
VERSION     DASPJI010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331793"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1697
                     /locus_tag="VEL74_14835"
     CDS             123..1697
                     /locus_tag="VEL74_14835"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HYG63851.1"
                     /translation="MFDWVHQVPILSIICYVPLVGALIVIFGIKGEKRALIRKFATGV
                     AFLDFLISIPLWFAFDRNGEGGELFQFQEKVPWIEAIGVDYWFGIDGIALLLVLLTTL
                     LGALAFLSSWSAIAVREKEYYVFMLLLQTGMLGVFMALDFFLFYVFWEVMLVPMYFLI
                     GIWGGPRRLYAAIKFFLYTLAGSVLMLLGILALYFFNAGGLLGWQGLGNDPTFSILRF
                     HEIGALIPPDLQIWIFLAFFVGFAVKVPMFPFHTWLPDAHVEAPTAGSVILAGVLLKM
                     GTYGFVRFSLPILPEATLELLPWMAGLSIVGILYGAMCAMAQKDMKKLVAYSSVSHLG
                     FVMLGLFALNGPGLNGAVLQMINHGLSTGALFLLVGVLYERRHTRLIADFGGLSAVMP
                     LYATIFLIITMSSIGLPTLNGFVGEFTILVGAFNRAWWWALLATLGIVLGAAYMLWLY
                     QRVFFGPLANPENQKLRDLDRRELLYLVPIVLLCFWIGLYPRPFFEVIRKPVDYVVAK
                     VDGSLGDTAVATAGRR"
     gene            1701..3152
                     /locus_tag="VEL74_14840"
     CDS             1701..3152
                     /locus_tag="VEL74_14840"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HYG63852.1"
                     /translation="MLPTVQDVLPLFPEILLAGAGLLLLLVGTIGPGLPNRGAAMVSV
                     AALVLTGFLLFRVREVVPDGTLILGGSFVLDGFSFFWKLLALMGTGLTVLLSVRFVEE
                     ARYRGTELYSLLLLATAGTMFMASGYTLLTIWISLETMALASYVLAGYFKRERRSAEA
                     ALKYFILGALSSGILLYGISLLYGAAGTVQLADLSIALGRARDNPLVPLGWLLLAAGL
                     FFKVAAVPFHVWAPDVYVGAPTPVTGWLAVVSKSASFAILLRIFYEGLGTLKPDWQWV
                     VALVAAATMIWGNLAALTQQNVKRMLAYSSIAHAGYVLIGVLAVSEIGLWSVLFYLLA
                     YTFITLGAFGTVILLERREYAGETVADYAGLSRRSPFLAAMMLVFLLALTGIPPTGGF
                     VGKVYLFAAAVEAGWSWVAVIGVVTSAVSLYYYAQIVVHMYLKDSELATPVPLRAPSV
                     VGVIGFCAFVTLLLGIVPGPFIELAKDSLLRLP"
BASE COUNT          404 a         1184 c         1065 g          702 t
ORIGIN      
        1 cagaccggct atgtctccaa ctacgcgctg gtgctggcgg ccggcgtctt cgcgctggtc
       61 tgcgtctata tggtgctgcg gctcacctga gccggagccc tgagttgcct ccggggggag
      121 tcatgttcga ctgggtccac caagtgccga tcctctccat catctgctac gtgccgctgg
      181 tgggtgcgtt gatcgtcatc ttcgggatca agggggagaa gcgggcgctc atccgcaagt
      241 tcgccaccgg cgtcgccttc ctggacttcc tgatctcgat ccccctctgg ttcgccttcg
      301 atcgcaatgg cgaggggggg gagctcttcc agttccagga gaaggtgccc tggatcgagg
      361 cgatcggcgt cgattactgg ttcggcatcg atggcatcgc cctgctgctg gtgctcctca
      421 ctacgctcct gggagccctc gcgttcctct cctcctggag cgcgatcgcc gtccgcgaga
      481 aggagtacta cgtcttcatg ctcctcctcc agaccggcat gctgggcgtg ttcatggccc
      541 tggacttctt cctcttctat gtcttctggg aggtcatgct ggtgccgatg tacttcctca
      601 tcggcatctg gggcgggccg cgccggctct acgcggcgat caagttcttc ctctataccc
      661 tggccggttc ggtgctgatg ctgctgggta tcctggccct ctacttcttc aacgccggcg
      721 gcctgctggg atggcaagga ctgggcaatg atccgacctt ctcgatcctg cggttccacg
      781 agatcggcgc cctgatcccc cccgacctcc agatctggat cttcctggcc ttcttcgtcg
      841 gcttcgccgt caaggtgccg atgttcccgt tccacacctg gctccccgac gctcacgtcg
      901 aggcgccgac ggcgggttcg gtgatcctgg ccggcgtcct gctcaagatg ggcacctacg
      961 gcttcgtgcg cttctcgctg cccatcctgc ccgaggccac gctggagctc ctgccctgga
     1021 tggccgggct gtccatcgtc ggcatcctct atggagcgat gtgcgcgatg gcccagaagg
     1081 acatgaagaa gctggtggcc tattcgtcgg tgagccacct cgggttcgtc atgctggggc
     1141 tcttcgccct gaacggcccg ggcctcaacg gcgccgtgct gcagatgatc aaccacggcc
     1201 tgtcgaccgg cgcgctcttc ctcctggtgg gcgtcctcta cgagcgccgc cacacgcgcc
     1261 tgatcgccga cttcggcggc ctctccgcgg tgatgcccct gtatgccacc atcttcctga
     1321 tcatcaccat gtcctccatc ggcctgccga cgctcaacgg cttcgtcggc gagttcacca
     1381 ttctggtagg cgcgttcaac cgcgcgtggt ggtgggcgct cctcgccacg ctgggcatcg
     1441 tcctcggcgc cgcctacatg ctctggctgt accagcgggt cttcttcggc ccgctcgcca
     1501 accccgagaa ccagaagctc cgtgacctgg accgccgcga gctgctgtac ctggtgccga
     1561 tcgtcctgct ctgcttctgg atcggcctct acccgcggcc gttcttcgag gtaattcgga
     1621 agccggtgga ttacgtggtc gccaaggtcg acggtagcct gggggacacg gccgttgcca
     1681 cggcggggcg gagataggcg atgctcccca ccgtacagga cgtgctcccg ctcttcccgg
     1741 agatcctgct cgccggggcg ggcctgctgc tcctgctcgt gggcaccatc ggcccgggcc
     1801 tgccgaaccg gggcgccgcc atggtctccg tcgccgccct ggtcctcacc ggcttcctgc
     1861 tgttccgggt ccgcgaggtc gtccccgacg gcacgctgat cctcggcggc tcgttcgtcc
     1921 tcgacggctt ctccttcttc tggaagctgc tggcgctgat ggggacgggg ctcacggtcc
     1981 tcctctccgt ccgcttcgtg gaagaggccc gctaccgcgg caccgagctc tactccctgc
     2041 tgctcctcgc caccgccggc acgatgttca tggccagcgg ctacaccctg ctgaccatct
     2101 ggatctcgct cgagaccatg gcgctggcga gctacgtcct cgccggctac ttcaagcgcg
     2161 agcggcgctc ggccgaggcg gcgctcaagt acttcatcct cggggcgctg tcgagcggca
     2221 tcctgctcta cggcatctcg ctcctctacg gagccgccgg gacggtgcag ctcgccgatc
     2281 tctccatcgc gctgggcagg gcccgggaca acccgctggt ccccctggga tggctcctcc
     2341 tcgccgccgg cctcttcttc aaggtggcgg cggtgccctt ccacgtctgg gcgccggacg
     2401 tctacgtggg ggcgccgacg ccggtgaccg gctggctggc ggtggtctcg aagtccgcct
     2461 ccttcgccat cctgctgcgc atcttctacg aggggctggg gacgctgaag cccgactggc
     2521 aatgggtcgt cgccctggtg gcggcggcca ccatgatctg ggggaacctg gcggccctga
     2581 cccagcagaa cgtcaagcgg atgctggcct acagctccat cgcccacgcc ggctacgtgc
     2641 tcatcggcgt gctggcggtg tcggagatcg gcctctggtc ggtgctcttc tacctgctgg
     2701 cctacacctt catcaccctg ggggccttcg gcacggtgat cctgctcgag cgccgcgagt
     2761 acgccggcga gaccgtggcc gactacgccg gcctgtcgcg gcgctcgccg ttcctggcgg
     2821 cgatgatgct cgtcttcctc ctcgcgctga cgggcatccc ccccaccggc ggcttcgtcg
     2881 gcaaggtgta cctgttcgcg gcggcggtcg aggcgggctg gtcctgggtg gcggtcatcg
     2941 gcgtggtcac cagcgcggtg tcgctctact actacgccca gatcgtcgtc cacatgtacc
     3001 tcaaggactc cgagctggcc acgccggtcc ccctgcgcgc cccatctgtc gtcggggtga
     3061 tcggcttctg cgccttcgtc accctgctgc tggggatcgt gcccggcccc ttcatcgagc
     3121 tggcgaagga cagcctcctg cggttgccgt agaataggga gaggggggcg gcattcccgt
     3181 cggtctcgcc gggggagggg gggtgccgaa tgggcagacc ggcaccgggg gaatgccgga
     3241 ctggcaggtc ttgttgcctc tctcaggcgt cctctgtcag tgacgtcggc gtccactttc
     3301 taccccgccc aggggacgat cccgcgtcct cttttgtccc cccatccgtt ggcac
//
LOCUS       DASPJI010000542         9194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_781175, whole genome shotgun sequence.
ACCESSION   DASPJI010000542 DASPJI010000000
VERSION     DASPJI010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9194)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781175"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VEL74_14845"
     CDS             <1..808
                     /locus_tag="VEL74_14845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HYG63853.1"
                     /translation="LADIGVDVVATGFLGADNAQPFEETFASQGIADHFVRVDGATRV
                     GLKIVDPGTQQATDINFPGLTPRPEQIEELLGRIAGLAGPDRWFVLSGSVPPGVPDGL
                     YARIIETVHKKKGHVVLDTSGRPLREAIAARPEVMKPNADELSELAGRALETTAELRA
                     AGELLLDGGVQRVVVSMGAGGALFVEDGEALLARPPRVEVRSTVGAGDAMVAGLLFAT
                     TQGLPLAEVARTATAAGAYAVTRIGSGVEDPAAWRELMAEVVIEPLTGRS"
     gene            1019..3493
                     /locus_tag="VEL74_14850"
     CDS             1019..3493
                     /locus_tag="VEL74_14850"
                     /inference="COORDINATES: protein motif:HMM:NF024972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4082 domain-containing protein"
                     /protein_id="HYG63854.1"
                     /translation="MRSARLLTAFILTILILLAAPGADAAPRRDGNDPQPLFVLDKAA
                     PGRGSLPLLPNSSEEIVIQVNPTDVAANPSSFLIDLPDHGLMEAVRTSFVSYRPDWKN
                     WFGTLRYASGKQETGSIHIGFHGATITASIQLDEGVFYRIVGGHGESHRLVRLDTEKT
                     PPSCGLATPEDAGDAPLPLVKPRHGKAALPVTEKILSCSANPRIDVLAVYPAKAPAGS
                     GSLVGYFDMTAAQESAFVTFVQNSISRANQVFANSGINAWYNLVGVVPLINQDVTSDP
                     PLPSTGLRAGLDWMNQRHTELRNLRDAFGADVVTVYIPFAWQGTANTADRACGMANLP
                     RTNDATFESAYGVVSQALDQRAFTVNRDTCGLDDFTLGHEIGHNYGMRHHDDPFNVSL
                     YVYPWAKGFVFNDPSSGQMRATMMGCNCPAGGCVAGTNPVCNRVPYLSDPDKIYNSQA
                     GVFMGARDVNGNDTHHNARVACDRVTGFSNFRAQSSNTPPNANFTVSCTGNGRTCSFN
                     AGSSTDNAAIPSNNYWWDFGDNTGIVRTGSTTTHTYASAGTYRVHLVVKDSGGQTNVE
                     TGTASPPSPSYEGYHESSSCSMISGWAWDQTLPNGAINVNIIRDGVSIASVPANQFRQ
                     DLLNAGKGNGYHGFGYVPNSSWFDGQWHSVGVRYGGTNTYLNGSPLPIPIICGAKMFP
                     GLTPDQNSSTGGQVYSVGTEFSSSQWGGQITKIGFYRAAGETGSNTLHLATNSGADLR
                     TVPTSCSSAGWCWVSITPYPVNAGTHYRVWVNTNTQQVKTPCGLTTSGLTNGPLTAHQ
                     GLWSAGNTAPTTGSCSNFFVDVQFDM"
     gene            3612..3914
                     /locus_tag="VEL74_14855"
     CDS             3612..3914
                     /locus_tag="VEL74_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63855.1"
                     /translation="MKSSALAFVLFALVALVPCPQLLAAEDVSGFLATLPQTEAPTVD
                     APAIEGAPDPTFLQTIIYCNSHSECPAGKLCCYPCGIDGCQNRCLTPVKGRCPLFV"
     gene            complement(3949..5694)
                     /locus_tag="VEL74_14860"
     CDS             complement(3949..5694)
                     /locus_tag="VEL74_14860"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYG63856.1"
                     /translation="MKRSAALFWFAVLCLLQPGRPAGAGEPYRAISLQPGLPDFGPAR
                     RLPIQEPRRAAIPEAPGAQAVELVCGKKKEADYACWLLLPQKEKGVAIVTLPLRQEES
                     GVLSVSINLHLERGSDAKTPLELRLLPAYNILLYQWSETIAARVVRGEALPDFAVTAL
                     DGKGWRISQLKGSVVVLSSWATWCVPCVEELPDLNRLAEKYAGAPVRFLAVASDTAEQ
                     VDGFLQQRPFRFEQSLATEESKRILGEGVPRHLVLDADGKVVEDLGGASASVTQQLES
                     VLQSLVAWAPAASPGARVVALGTVQDGGMPQTGCDCSRCAAARKSPALRRHVASLLLR
                     AGGRDYLVDATPDLPAQIERIHRTRRHPAGKVDRAPVDGVLLTHAHIGHYLGLAHFGF
                     ESLNTKDIPVYVTPRMAEYLRTSGPWSQLVKLGNIALREVRPGTPFELDGGLTVTPLQ
                     VPHRDEYSDTMAFVIRGPKKALLYVPDTDSWREWARPLPEVLEAEKIDFALLDATFYS
                     PDELPDRDVTKIKHPLITDSMDLLEPLVKAGKVRVWFTHLNHSNPALDVNSSARRVIE
                     RRGFRVLAEGEEIEL"
     gene            5793..6197
                     /locus_tag="VEL74_14865"
     CDS             5793..6197
                     /locus_tag="VEL74_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG63857.1"
                     /translation="MAPRIVLDTSAVIAVITNEAHKPELVRVTRGMELLAPSSLPAEI
                     GNAFSAMFKRKRISLEQARAAIAAYRQIPIRFTDIDLDQSLEISEKLGIYAYDAYVIA
                     CGLQHRAALVTLDRGQREAAEKAGAAVLEVPV"
     gene            6194..6418
                     /locus_tag="VEL74_14870"
     CDS             6194..6418
                     /locus_tag="VEL74_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HYG63858.1"
                     /translation="MTVYTFSEAQQKLAFILDEAERQGAVRIKSRDGSEFEIAPVREE
                     RSPLDIEGVPLDLTAEEIVAALKESRSRNS"
     gene            complement(6533..6787)
                     /locus_tag="VEL74_14875"
                     /pseudo
     CDS             complement(6533..6787)
                     /locus_tag="VEL74_14875"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
     gene            6765..6899
                     /locus_tag="VEL74_14880"
     CDS             6765..6899
                     /locus_tag="VEL74_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63859.1"
                     /translation="MRGDIQIIERSSSSLFALLSIQPKQSASSTASPYSTRGLPVSFL
                     "
     gene            7095..7595
                     /locus_tag="VEL74_14885"
     CDS             7095..7595
                     /locus_tag="VEL74_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63860.1"
                     /translation="MRHPWKSTAVLATALVWAAMPSAGQQVTSVEMTFYGADRATYGS
                     WAPTPADLFVELVPSVEATGGDILGSVRLQTDPEEMSRSKGSASVDFRLAPDETPAKL
                     RERVEAHLRQQGFHLVSEEPDLETRRAHPRIVCLTWDTDEPGAQTSLDLPPRARRCGA
                     VVLPVH"
     gene            complement(8115..8375)
                     /locus_tag="VEL74_14890"
     CDS             complement(8115..8375)
                     /locus_tag="VEL74_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63861.1"
                     /translation="MSNHDDGSTRYPSVPSNVFSGDFLKRFDEEDEPPTALEAELAGV
                     GTVFPVPEGHALYLPGEALLQAAGKVTLERVGTLLARRVPAR"
     gene            complement(8850..>9194)
                     /locus_tag="VEL74_14895"
     CDS             complement(8850..>9194)
                     /locus_tag="VEL74_14895"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HYG63862.1"
                     /translation="TRESKAPAFWKRATLERQSEETLITNVYTGLYARGIRNRFADDY
                     QTSGAAVLPGLLQSTAAQDIYAAASAKGDGDYYPMLSGQSLGLIRDLPGAAEVVERVV
                     EEARAAMARLPR"
BASE COUNT         1539 a         3238 c         2971 g         1446 t
ORIGIN      
        1 cctggcggac atcggggtgg acgtcgtcgc caccggcttc ctcggcgcgg acaacgccca
       61 gccgttcgag gagacgttcg cgagccaggg gatcgccgac cacttcgtcc gcgtcgacgg
      121 cgccacccgc gtcggcctca agatcgtcga ccccggaacg cagcaggcga ccgacatcaa
      181 cttccccggc ctcacccccc ggccggagca gatcgaggag ctcctcggcc ggatcgccgg
      241 gctcgccggg ccggaccgct ggttcgtgct cagcggcagc gtccccccgg gggtgccgga
      301 cggcctctac gcccggatca tcgaaacggt ccacaagaag aaggggcacg tggtgctgga
      361 caccagcggc cggccgctgc gcgaggcgat cgccgcccgg cccgaggtga tgaagccgaa
      421 cgcggacgag ctgagcgagc tcgcggggcg cgccctggag acgaccgccg agctgcgcgc
      481 cgccggcgag ctgctgctgg acggcggcgt gcagcgggtg gtggtgtcga tgggcgccgg
      541 cggcgcgctc ttcgtggagg acggcgaggc cttgctggcc cggccccccc gggtggaggt
      601 gcggagcacc gtcggggcgg gcgacgccat ggtggccggc ctcctcttcg ccacgaccca
      661 ggggctgccg ctggcggagg tggcgcggac cgccaccgcc gccggcgcct acgcggtgac
      721 ccggatcggc tccggggtcg aggacccggc cgcctggcgc gagctgatgg ccgaggtcgt
      781 gatcgaacct ctgacgggac ggagctgaac catgacgaag cggatcgcga gcatcaactc
      841 ctggtcgacc agccggcggc cggccctccg gagcccgatc tcctccctga tgacagaccg
      901 cgcctcaacg tgtcgcatct ccagctagag cccctccact ttcctcgaga ccaatctatg
      961 gaggggcctg cggacgcgcg ctgttgcgca tcccattttc cagcaggaga tccgacccat
     1021 gcgaagcgca aggctcctca cggccttcat cctcaccatt cttatcctcc tggccgcgcc
     1081 gggcgccgac gccgcccccc gccgggacgg caacgatccc cagccgctct tcgtgctcga
     1141 caaggcggca ccggggaggg gcagcctgcc gctgctgccc aactccagcg aggagatcgt
     1201 catccaggtg aatcccaccg acgtcgccgc gaatccatcg tcgttcctga tcgatctccc
     1261 cgatcacggc ctcatggagg ccgtgcgtac gagcttcgtc agctaccggc cggactggaa
     1321 gaactggttc ggcactttgc ggtacgccag cggcaagcag gagacgggct cgatccacat
     1381 cggcttccat ggcgccacga tcacggcctc catccagctc gacgagggcg tcttctatcg
     1441 catcgtcggc ggccacgggg agagccaccg gctggttcgt ctcgacaccg agaagacccc
     1501 gccatcgtgc ggcctggcaa ccccggagga tgccggcgat gcgccgctcc ccctggtcaa
     1561 gccgcgtcac ggcaaggccg ccctgccggt caccgagaag atcctctcct gctccgccaa
     1621 cccccggatc gacgtgctcg ccgtctatcc ggccaaggct ccggcgggga gcggctcgct
     1681 ggtcgggtac ttcgacatga ccgccgccca ggagagcgcc ttcgtcacct tcgtccagaa
     1741 ctccatctcc agggccaacc aggtcttcgc caacagcggg atcaacgcct ggtacaacct
     1801 ggtgggcgtc gtgccgctca tcaaccagga cgtcacgagc gatccgccgc tgccgtccac
     1861 cggcctgcgg gcgggcctcg actggatgaa ccagcgccat accgagctga ggaacctccg
     1921 cgacgccttc ggcgccgacg tcgtcacggt ctacatcccc ttcgcgtggc aggggaccgc
     1981 gaacaccgcc gaccgggctt gcggtatggc caacctgccg cggaccaacg acgccacgtt
     2041 cgagtcggcc tacggcgtcg tgtcccaagc tctggaccag cgcgccttca ccgtcaaccg
     2101 cgacacctgc ggcctggacg acttcaccct gggccacgag atcggccaca actacggcat
     2161 gcgtcatcac gacgacccgt tcaacgtgtc gttgtacgtc tacccctggg ccaagggctt
     2221 cgtcttcaat gatccgtcgt ccgggcagat gagagccacg atgatggggt gcaactgccc
     2281 ggccggcggc tgtgtcgcgg ggaccaaccc ggtctgcaac cgggtccctt atctttcgga
     2341 tcccgacaag atctacaaca gccaggcggg cgtgttcatg ggcgctcgtg acgtcaacgg
     2401 caacgacacc catcacaacg cgcgggtggc ctgcgatcgg gtcacgggct tttccaactt
     2461 ccgggcacag agctcgaaca ctccgcccaa cgccaacttc acggtctcct gcaccggcaa
     2521 cggccgcacc tgctccttca atgccggctc cagcaccgac aacgcggcga tcccgtcgaa
     2581 caactattgg tgggacttcg gagacaacac gggcatcgtc cgcaccggca gcaccaccac
     2641 ccacacgtat gcctctgccg gcacctatcg ggtgcatctc gtggtgaagg acagcggcgg
     2701 ccagaccaac gtcgagacgg ggacggcgag cccgccctcg ccgagctacg agggctacca
     2761 cgagagctcc tcgtgcagca tgatctccgg ctgggcgtgg gaccagaccc ttcccaacgg
     2821 agcgatcaac gtcaacatca tccgcgacgg cgtcagcatc gcgtccgtgc cggcgaacca
     2881 gttccgtcag gacctcctca acgccggcaa ggggaacggc tatcacggct tcggctacgt
     2941 gcccaacagc tcgtggttcg acggccagtg gcacagcgtc ggcgtgcgct acggcggtac
     3001 gaacacttac ctcaacggca gcccgctgcc gatcccgatc atctgcggcg cgaagatgtt
     3061 ccccggtctc actcccgacc agaacagctc cactggcggc caggtctact ccgtgggcac
     3121 ggagttcagc tccagccaat ggggtggcca gatcaccaag atcgggttct atcgcgcggc
     3181 aggagagacg ggctccaaca ccctccacct cgcgacgaac agcggcgccg atctcagaac
     3241 cgtgccgacg agctgctcct ccgcgggatg gtgctgggtc tcgatcacgc cgtaccccgt
     3301 caacgccggg acccactacc gcgtctgggt caacaccaac acccagcagg tcaagacccc
     3361 ttgcggcctc accacgagcg gcctgaccaa cggccccctg accgcgcacc agggcctctg
     3421 gagcgccggt aacaccgccc cgaccaccgg gagctgcagc aacttcttcg tcgacgtgca
     3481 gttcgatatg taacggtgcc cgttcgggtc ccgtcatcag acgggacccg aacgcgctcc
     3541 gcggtgccgt gagcacctac tccttgtaga cggccggcgt catgccggct gcccctcaag
     3601 gagatgaagt catgaaaagc tccgccctcg ccttcgtcct cttcgccctc gtcgctctgg
     3661 tcccctgtcc gcagctcctg gccgccgagg atgtctcggg cttcctggcc accctgccgc
     3721 agaccgaggc ccccaccgtc gatgcccccg ctatcgaggg agcgcccgac cccacgttcc
     3781 tccagacgat catctactgc aacagccact ctgaatgccc ggcgggcaag ctgtgctgct
     3841 acccctgcgg tatcgacggc tgccagaacc ggtgtctgac gccggtcaag gggcgctgcc
     3901 cgctcttcgt gtaggagcct ccagcggcaa gcgggaggag acagtccctc agagctcgat
     3961 ctcctccccc tccgccagca cccggaagcc ccgcctctcg atgaccctgc gagcggagct
     4021 gttcacgtcg agggcggggt tggagtggtt gaggtgggtg aaccagaccc ggaccttgcc
     4081 ggccttgacc agcggctcca ggaggtccat ggagtcggtg atgagcgggt gcttgatctt
     4141 ggtgacgtcg cggtccggca actcgtcggg ggagtagaag gtggcgtcga gcagggcgaa
     4201 gtcgatcttc tccgcctcca gcacctccgg cagcggccgg gcccactcgc gccaggagtc
     4261 ggtgtccggg acgtagagca gggccttctt cggcccgcgg atcacgaacg ccatggtgtc
     4321 cgagtactcg tcccggtgcg gcacctggag cggcgtcacc gtgagccccc cgtccagctc
     4381 gaagggcgtc ccggggcgga cctcgcgcag ggcgatgttc cccagcttga ccaactggct
     4441 ccacgggcca ctggtccgca ggtactccgc catgcgcggc gtcacgtaga cggggatgtc
     4501 cttggtgttc aggctctcga acccgaagtg ggcgagcccc aggtagtggc cgatgtgggc
     4561 gtgggtgagg agcacgccgt cgaccggcgc gcggtccacc ttgcccgcgg gatggcggcg
     4621 ggtccggtgg atcctctcga tctgggccgg caggtccggc gtcgcgtcca ccaggtaatc
     4681 ccgtccgccg gcccggagga gcaggctcgc cacgtggcgt cgcagggccg ggcttttccg
     4741 tgccgccgcg cagcgggagc agtcgcaacc cgtctgcggc atgccgccgt cctgcaccgt
     4801 tcccagggcg accacccgcg cgcccggact ggcggccggg gcccaggcga cgagcgactg
     4861 cagcacggat tcgagctgct gggtgaccga ggccgaggcg ccgcccaggt cttcgacgac
     4921 cttgccgtcc gcgtcgagga cgaggtgccg cggaacgcct tcgccgagga tccgtttgct
     4981 ctcctccgtg gcaaggctct gctcgaaccg gaatggacgc tgctgcagga agccgtcgac
     5041 ctgctcggcg gtgtcgctcg cgacggccag gaaacggacc ggagcaccgg catacttctc
     5101 ggcgagccgg ttgagatccg ggagctcctc gacgcaggga acacaccagg tggcccagga
     5161 gctcaggaca acgaccgagc ccttgagctg gctgatgcgc cagcccttgc cgtccagggc
     5221 cgtcaccgcg aaatccggca gcgcctcccc ccggaccaca cgcgcagcga tggtctcgga
     5281 ccactggtac aggaggatgt tgtacgccgg caggaggcga agctccagcg gggtcttggc
     5341 atcggagccc ctctcgaggt gaaggttgat gctgacggag agcaccccgg actcctcctg
     5401 acggagcggc agcgtcacga tcgccactcc cttctccttc tggggaagga gaagccagca
     5461 ggcgtagtcg gcttccttct tcttgccaca gaccagctcg acggcctggg cgcccggagc
     5521 ctcggggatg gctgccctcc gcggctcctg gatgggcagc cgccgggcgg gcccgaagtc
     5581 cggcagcccg ggctgcaggg agatcgcgcg gtagggctct ccggctccgg ccggccgccc
     5641 gggctggagc aggcagagga ctgcgaacca aaagagggct gcgcttctct tcatcggagt
     5701 cgtcctccct gggctggaga ggccttcatg aacaatgtta gcaggatgac agccgccgga
     5761 agagacagtc gtactagtac acttgtctcc ctatggctcc ccggatcgtc ctcgacacct
     5821 ctgccgtgat cgcggtgatc accaatgaag ctcacaagcc ggagttggtc cgggtcaccc
     5881 gcgggatgga gttactggca ccatcgtccc tcccggcgga gatcggcaac gccttctcag
     5941 ccatgttcaa gagaaagcgc atctctctgg agcaggcgag agcagccatt gccgcctacc
     6001 ggcagatacc catccggttc accgacatcg atctggacca aagtctggag atcagcgaga
     6061 aacttggcat ctacgcctac gatgcttacg tcatcgcatg cggcctccaa catcgagccg
     6121 ctctggtcac gctggatcgc ggccagcggg aagcggcaga gaaggctgga gccgccgtgc
     6181 tggaggtccc tgtatgacgg tctacacctt ttcggaagct caacagaagt tggcgttcat
     6241 cctggatgaa gcggagcgtc aaggcgctgt ccgcatcaag agccgcgatg gcagcgagtt
     6301 cgagatcgca ccggtccgtg aggagcgctc tcctctggac atcgagggag tcccgctcga
     6361 tctgacggcc gaggagatcg tcgccgcgtt gaaggaatct cgcagccgga acagctaact
     6421 ttcctgccgc cactggttgg cccggacgtt ctcctccgcc aagcgggcgg tcctcgaccc
     6481 gcttggcccg ggtctcgggc ttcttcgccg tcgagatcca ctccaggatg gcacgcttga
     6541 ccgagcgggg gaaggattcg aagtgcttcc acgcggaggg ataggcgtcc aacgcctggg
     6601 cgaggccggc ggggatctcc agggcctcga cggcgtcgag cgcgttccag gagccatcct
     6661 gcttcgcggc ctcgatcttc gcgagacccg gaggcgcgat cagtccctgg gcgagcagcc
     6721 tctccacccg ctccttgttg ctccgggacc agccggtccc gggcttgcgc ggcgacatcc
     6781 agatcatcga gcgctcgtcg tcgagcttgt tcgccttgct gtcgatccag ccgaagcaga
     6841 gcgcctcctc caccgcctcg ccgtactcca cccgcggctt gcccgtgtcc ttcttgtagc
     6901 tcacgagcca gatccctgcg gtccggttgt ggttcgcttc cagccacgcc cgccaatccg
     6961 ccctgctctt cgggtgcgtc gaattctccg gcgccttcgt catcgctccc ccctcctcta
     7021 ggaaaggatg ctatcaagag gacgggatgc tcgcctccgg gataaccggt agaatgatgg
     7081 ctagggagga gtccatgaga cacccttgga aatccacggc cgtcctcgcg acggcgctcg
     7141 tgtgggcagc gatgccaagc gccggacagc aggtgacctc ggtagagatg accttctacg
     7201 gggcggaccg cgccacctac gggagctggg cgcccacccc ggcggacctg ttcgtcgagc
     7261 tggtccctag cgtagaggcc accggcggcg acatcctcgg cagcgtgcgc ctgcaaacgg
     7321 atccggagga aatgagcagg agcaagggct cggcctcggt cgacttcagg ctggcccccg
     7381 acgagacgcc agcgaagctc cgcgaacgcg tggaagcgca cctgcgccag caaggatttc
     7441 acctcgtctc ggaggagccc gacctggaga cccgccgtgc gcacccccgc atcgtctgcc
     7501 tgacctggga taccgacgag cctggcgccc agacctccct ggacctgccg cctcgcgcga
     7561 gacgctgcgg agccgtcgtc ctcccggtcc attaagacca gcggccagcc tgccgctccc
     7621 gaggacctgc gcctaccggc tcgccggcac ggctgggggt ggcctcgcaa caatcctccg
     7681 cgactccaaa gccttcaccg cgacccaaaa ggactcgccg cgactcccat cgcttcgccg
     7741 cgacctcatt ggcggcgccg cgacttcatt cgacctgccg caagcccgct tggttcgccg
     7801 cgacttcgaa ggcttcaccg caaccttgaa gaactcgccg caagatcggc ccgttcaccg
     7861 caacttcatt tgactcaccg cgacttcgtt tcattcgccg taagatcatc tagttcaccg
     7921 caagatcatt ggcttcgccg caagcccgtt cggttcaccg caagctcatt tcgttcgccg
     7981 cgacttgatc tcccgcaccg cgacttcaaa cgcttggccg tgattgcagc cggcgcttgc
     8041 cggcggagaa gaagagaaat ggggtctgga agccgccccc tcccacccaa gccggcggga
     8101 gggggcagaa gatgtcagcg cgccggaacg cggcgagcga ggagggttcc cacccgttcc
     8161 agggtcacct tgcccgccgc ctggaggagc gcctctccgg gcaggtacag cgcgtggcct
     8221 tccggcaccg ggaagacggt ccccaccccg gccagctccg cctcgagagc ggtcggcggc
     8281 tcatcctctt cgtcgaagcg cttcaggaag tcgccggaaa agacgttgga cgggacggac
     8341 ggataccggg tgctaccatc atcgtggttg ctcataggaa tctccattcc tgtgtgtgac
     8401 ctaggtccgg ggtgagccgc gaactcacct cgggccgtct ggccacgggc tgggtacagc
     8461 gaagctacgg tgaaagtata aaacgggagc ggcggcgggt caagagggaa agcgcggatg
     8521 cgggagattc ttcgcccccg ggggcgcctc acaacgccgg gcagatctcg ttgaccgcca
     8581 cttggaagcc agacagcacc gccgaccaca cgccgagccg acgcgatgct ccatcgagcc
     8641 ggctggatgg aacgtcgagc cgacgagatg gaacgccgag ccgacgcgat gctccatcga
     8701 gccaaagaga tgacacgtcg cgccggcggg atgagacgct gagccgacgt gatgctccat
     8761 cccgccggcg cgatggaacg tccaggcggc gggtcgtggt accgggccgg gggaggaaag
     8821 gaaagggcgc caccggccct acagccacct caacgcggca gccgggccat cgccgcccgc
     8881 gcctcttcca ccacgcgttc caccacctcg gcggctccgg ggaggtctcg gatcaatccc
     8941 aggctctggc cggagagcat ggggtagtag tcgccgtctc ctttggcgct ggcggcggcg
     9001 tagatgtcct gggcggcggt ggactggagg aggccgggga ggaccgcggc tcccgaggtc
     9061 tggtagtcgt cggcgaagcg gttgcggatg cctcgggcat agaggccggt gtagacgttg
     9121 gtgatcagcg tctcctcgct ctggcgctcc agcgtcgccc gcttccagaa ggccggcgcc
     9181 ttggactcgc gggt
//
LOCUS       DASPJI010000543         7678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_435325, whole genome shotgun sequence.
ACCESSION   DASPJI010000543 DASPJI010000000
VERSION     DASPJI010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7678)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7678
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435325"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VEL74_14900"
     CDS             <1..543
                     /locus_tag="VEL74_14900"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG63863.1"
                     /translation="VIGTVFAGRNRPELTGLISLFLNTLPVRSDLSGRPAFRDLLLQT
                     RDAVLEAYAHQDAPFPRLIAELYPGQAPDRQLLFRVVVNLLDFEFAGGNPAEEAGPAG
                     LSIEPMTHGELWATYDLALDIAGGEDTLVCQMVAAADLVTPEGLAAIKLDLEALLEQA
                     VAAPGTPLGLLLPEPRHRPA"
     gene            623..1273
                     /locus_tag="VEL74_14905"
     CDS             623..1273
                     /locus_tag="VEL74_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016870209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HYG63864.1"
                     /translation="MMTTSVRKLLALPLLLAAAFFLPTPTPLAQTTRPAQAQAVHAEM
                     APDRVAELIYAEIQQIHGYRSTPRLHFGVRAGRTRSSCGRIDGSGYCPVDHAVFITSR
                     DIAMAYQHGDAALAFIIAHEYAHAMQTAFRFMPRITPMAELQADCLAGVYLGSLPNVT
                     YDRSDMREIGTLAYRIGDYRWGRLHHGTPDQRVQAVLHGIGASRDGLEGARACMAS"
     gene            complement(1806..2171)
                     /locus_tag="VEL74_14910"
     CDS             complement(1806..2171)
                     /locus_tag="VEL74_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63865.1"
                     /translation="MRRSTLVRLMLVLALTAGLFVGLAPGLQAVTGGTPTSCPSVSCL
                     TQEGYDKCTGAGCGCDPRNDWCVPKATPTPTTPGCPSTCMPESAYNACKECGGSCGTR
                     DECIPPSPTPSGTATPYTY"
     gene            complement(2217..2471)
                     /locus_tag="VEL74_14915"
     CDS             complement(2217..2471)
                     /locus_tag="VEL74_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63866.1"
                     /translation="MNAMLRILGLSTILVCTVLSSAGADTSGNCQTLCFGSGITQVVW
                     PSTEEECCSRSVNPCPPGSSPSSGYAWAPDNGFWGFCPPI"
     gene            complement(2642..4405)
                     /locus_tag="VEL74_14920"
     CDS             complement(2642..4405)
                     /locus_tag="VEL74_14920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG63867.1"
                     /translation="MPFEIQKPVLRQFLSFLLLLSAAFHPAAAQAPAPPATEESEETF
                     YDTFDVSVVNVEVFVTDRQGNRVTGLTRDDFQILEDGKPVEITNFYAAEATAAAPAPG
                     PNPGAGGRALPDALQEVQSLQLAVYVDTTNLSSASGQRLRKSLEEFLASRAGEQILIA
                     SYGGADELKVRPVDTADPRAVAAAVGEVFTGLPGQPHRVMQLRQLLRETQLGKDPTSV
                     RSDADWSEVTLDEARISASVESYVQQVQRDAEVSIGALDHFLNGLAGLPGRKAVLHVS
                     GGLPIHPGEAMGGAVGKKYWGYFRRQFDGRSQTDLEARLRQVEERANANRITLYGLGQ
                     SDNVAPNYGWNPYGLWAHREEESERSGLARSLDEMALPTGGLADLNAVNPGALLGQMG
                     QDLDSYYSLGYTPAHRRPGTTHALKVKVKRPDLVVRHRQSYRDRTPAEVLADRTVSAL
                     YFGTKGNPLELAVQIDGEGELEKDQQKVKLTLKMPFSKLVVLPQGNVHQGRLQVAVAV
                     RDAAGRTSKVTTFEVPVRIPNDRLQEMFSQTVGYETRLAMRSVEHVVAVSVRDELGNL
                     SSTVTTTWAPPAGGACQTSSR"
     gene            4698..5213
                     /locus_tag="VEL74_14925"
     CDS             4698..5213
                     /locus_tag="VEL74_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63868.1"
                     /translation="MNPKHFLALFLLPTLAAPAAAQVSRGADPCLEARRIAESPACLA
                     GAADCQAAQRWQQVFGTPGTGLADPQRTGGLAGKEVRLEATEDLRSVLTRLAAAAGLE
                     LLTGPRVTGELRAEAGTLPLGEAWRKVLDTAGLVARFEEGKVLVARADRAQGGTPRRQ
                     RSATLFNCPTR"
     gene            complement(5226..5483)
                     /locus_tag="VEL74_14930"
     CDS             complement(5226..5483)
                     /locus_tag="VEL74_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63869.1"
                     /translation="MKSMLRILSLSAVLASAALTSASASYTGTCRTFCSGSGFTTVTW
                     SATKSRCCSLLGAPCPSGTTATGASWAGSGGLWQFCGPTVE"
     gene            5746..>7678
                     /gene="aceE"
                     /locus_tag="VEL74_14935"
     CDS             5746..>7678
                     /gene="aceE"
                     /locus_tag="VEL74_14935"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HYG63870.1"
                     /translation="MARNGRTTPLNWYTGGVPDTDVKETEEWLTAFEQVIQYEGRERA
                     QFLLKKLLEKGYEKDVTLPFTGNTPYINTIPASKEPSYGGDRAIERRIKSIMRWNAMA
                     MVVRANKKHNGLGGHISTFASAATLYQVGLNHFFRGRGESGFEGDMLYIQGHASPGIY
                     AQAFLEGRITVEQMEAFRREVEVKGGLSSYPHPWLMPDFWEFPTVSMGLGPITAIYQA
                     KFNRYLRDRGLRDPQNRKIWAFLGDGETDEPETLGAITLAAREQLDNLIFVINCNLQR
                     LDGPVRGNGKVIQELETAFRGAGWNVIKVIWGSDWDPLIEADNTGLLIQTMNETLDGE
                     YQKYVVEPGSYIRQHFFGKHPELLKLVEGYSDEDLHALRRGGHDPVKVYAAYKAAVEH
                     EGSPTVILAKTVKGYGMGEAGEGRNVTHQQKKLNEEELRFFRDRFEIPVSDKDLAEAP
                     FYCPPEDSEEIQYLRERRRALGGYVPSRRVRKEPSPMPAESTWGRFYEGVDRDVSTTM
                     VFVQILTQLMKDKEIGTRVVPIVPDEARTFGMESLFRQFGIYSHVGQLYEPVDKGMLL
                     YYREATDGQILEEGITEAGSMASFIAAGTSYATHGIDMMPFFIFYSMFGFQRVADLIW
                     AGADSRMRGFLLGATAGRTT"
BASE COUNT         1209 a         2679 c         2636 g         1154 t
ORIGIN      
        1 gtcatcggca cggtcttcgc ggggaggaac cggccggagc tcacggggct gatcagcctc
       61 ttcctcaata ctctgccggt gcgctccgac ctctccgggc ggccggcgtt ccgggacctg
      121 ctgctgcaga cgcgcgacgc ggtgctcgag gcctacgcgc atcaggacgc ccccttcccg
      181 cgcctgatcg ccgagctcta ccccggccag gcgcccgacc gccagctcct cttccgggtc
      241 gtcgtcaacc tgctcgactt cgagttcgcg ggcggcaacc cggccgagga ggcgggtccg
      301 gcggggctct cgatcgagcc gatgacgcac ggggagctct gggcgacgta cgacctggcg
      361 ctcgacatcg cgggcggcga ggacaccctg gtctgccaga tggtggccgc cgccgacctc
      421 gtcactccgg aggggctcgc cgccatcaag ctggacctgg aggccctgct cgagcaggcc
      481 gtcgccgccc ccggcacccc gctcggcctc ctcctccccg agccgcgcca ccggccggcc
      541 tgaggggatg aaaggagcat cgccgccccg gaaacaacca ccccatcctt ctttgttctt
      601 cctcccagta gatggaggtc cgatgatgac gacgagtgtc cgcaagctcc tcgccctgcc
      661 gctgctcctc gccgcggcgt tcttcctgcc cacccccacg cccctggctc agacaacccg
      721 gccggctcag gcgcaggccg tccatgccga gatggcgccc gaccgggtcg ccgagctcat
      781 ctacgccgag atccagcaga tccacggcta ccggtcgacc ccccgcctgc acttcggcgt
      841 ccgggccggc cgcacccggt cgtcctgcgg caggatcgac ggcagcggct actgccccgt
      901 cgatcacgcg gtgttcatca cctcgcggga catcgccatg gcctaccagc acggcgacgc
      961 ggccctcgcc ttcatcatcg cccacgagta cgcccacgcc atgcagacgg ccttccggtt
     1021 catgccccgg atcaccccga tggcggagct gcaggcggac tgcctggccg gggtctatct
     1081 cggctcgctg cccaacgtca cctacgaccg gtcggacatg cgggagatcg gcaccctggc
     1141 ctaccgcatc ggcgactacc ggtgggggag gctgcatcac ggcacgcccg accagcgcgt
     1201 gcaggcagtc ctccacggca tcggcgccag ccgggacggg ctcgaagggg cccgcgcctg
     1261 catggcgtcc tagcaccgga agcccaacgc tcccgggcgc ggcgccatcc ttgcccatcc
     1321 ggcggccgcg cccgggaacc gggtccatcg attgtcaaag aaccttcgcg ggcccgtgcc
     1381 gcgcggttgg acccgcgccg gacctgtttc gaccctcctc gttcctcgcg cgaccctccc
     1441 cgtttccctc tcgaccctgg ggtcacgagg ctccacgctc ttcgcaccca cttcgacccg
     1501 agtcgaaccc cgcgtgaccc gcgggtcgag agcctggacc cccgggtcga aaccactcgg
     1561 aaccgggtcc gatgattcga cccgagtgaa aacagtctcc acaacccgtc gggtccgcgt
     1621 cggacatcga agctacatcc ctattaggcc gcatcaggcc ggaaaagtca atttcggtct
     1681 tgccacgggg ccggagtccc gtgtagagtg ggccggctgg tgggttcctt gaaaactgaa
     1741 tcggacgggg aggcggaggg ctgagcccgc cgcctcccgg ggctggtggg gatggtgcgc
     1801 ccggtctagt aggtgtaggg cgtcgccgtc cccgaaggcg tgggcgacgg cgggatgcac
     1861 tcgtcccgcg tgccgcagga gccgccgcac tccttgcagg cgttgtaggc cgattccggc
     1921 atgcaggtcg aggggcagcc cggcgtcgtg ggtgtcggtg tggccttggg cacgcaccag
     1981 tcgttgcgcg ggtcgcagcc gcagccggcg ccggtgcact tgtcgtagcc ctcctgggtc
     2041 aggcacgaga cgctggggca ggacgtcggg gtgccgccgg tcaccgcctg cagcccgggg
     2101 gcgaggccga cgaacagccc cgcggtgagc gccagcacca gcatcagacg gacgagagtc
     2161 gatctcctca tggaacctcc ttggacagac aagagacagc cgttatcggc gcccgatcaa
     2221 atcggcgggc agaaccccca gaatccattg tccggagccc aggcgtatcc cgaggagggg
     2281 gacgagccgg gcgggcaggg attcaccgag cggctgcagc actcctcttc ggtggaaggc
     2341 cagacaacct gggtgatccc ggagccgaag cagagggttt ggcagttgcc ggaggtgtcg
     2401 gcgcccgcgc tcgacaagac cgtgcagaca agaatcgtgg acaggccgag aatccgcagc
     2461 attgcgttca agagatctcc tcctgcgagc ggtccggagc ggctggaacg cccttatggt
     2521 gagaggcccg gtcatcgggc gctcatgtct taccaggtgc ttcgagtgac cgctggcgcg
     2581 ctccagctcc ctctcgaaca gagtgccggc ggtcacccgg ggccagactc tgcgagagag
     2641 gtcagcgcga tgaggtctgg caggcccctc cggccggcgg tgcccaggtc gtggtcaccg
     2701 tggacgagag gttgccgagc tcgtcgcgga ccgagaccgc caccacgtgc tccacggagc
     2761 gcatcgccag ccgggtctcg tagcccaccg tctggctgaa catctcctgc agccggtcgt
     2821 tggggatccg caccggcacc tcgaacgtgg tgaccttgga ggtccgtccg gcggcgtcgc
     2881 gcaccgcgac ggccacctgc agccggccct ggtggacgtt cccctgcggg agcaccacga
     2941 gcttcgagaa gggcatcttc agggtgagct tgaccttctg ctgatccttt tccagctccc
     3001 cttcgccgtc gatctgaacg gcgagctcga gcgggttgcc cttggtcccg aagtagaggg
     3061 ccgagaccgt ccggtcggcg agcacctccg cgggggtgcg gtcgcggtag ctctgccggt
     3121 gccggacgac gaggtccggc cgtttgacct tcaccttgag ggcatgggtg gtgcccggcc
     3181 gccggtgcgc cggcgtgtag ccgagcgagt agtaggagtc caggtcctgt cccatctgcc
     3241 ccaggagggc gccgggattg accgcgttga ggtccgccag cccgccggtg gggagcgcca
     3301 tctcgtcgag cgaccgcgcg aggccgctgc gctcggactc ctcttccctg tgcgcccaga
     3361 ggccgtaggg gttccagccg tagttcggcg cgacgttgtc cgactggccc aggccgtaga
     3421 gggtgatgcg gttggcgttg gccctctcct cgacctggcg caggcgggcc tcgaggtcgg
     3481 tctgggaccg cccgtcgaac tggcggcgga agtagcccca gtacttcttg ccgacggccc
     3541 cgcccatcgc ctcgccgggg tggatcggca ggccaccgct cacatggagc accgccttgc
     3601 gccccggcag gccggcgagc ccgttgagga agtggtcgag ggcgccgatc gacacctcgg
     3661 cgtcgcgctg gacctgctgg acataggatt ccacgctcgc cgagatgcgt gcctcgtcga
     3721 gcgtcacctc ggaccagtcc gcgtcgctgc ggacgctggt ggggtccttg ccgagctggg
     3781 tctcgcgcag cagctggcgg agctgcatca cccggtgcgg ctggccgggg aggcccgtga
     3841 agacctcacc gaccgcggcg gccaccgccc gcgggtccgc ggtgtcgacc ggccggacct
     3901 tgagctcgtc ggcgccgccg tagctcgcga tcagaatctg ctcgccggcg cgggaggcga
     3961 ggaactcttc cagcgacttc cgcagacgct gtccggaggc cgacgacagg ttcgtcgtgt
     4021 cgacgtagac ggcgagctgg aggctctgca cctcctgcag ggcgtccggc agggcgcggc
     4081 cgccagcgcc ggggttcggg cccggcgccg gcgcggcggc cgtggcctcc gcggcataga
     4141 agttggtgat ctcgaccggc ttgccatcct cgaggatctg gaagtcgtcc cgcgtcaagc
     4201 cggtgacgcg gttcccctgg cggtcggtga cgaagacctc gacgttgacg acgctgacgt
     4261 cgaaggtgtc atagaaggtc tcctcggact cctcggtcgc cggcggtgcg ggcgcctgcg
     4321 cggcggcggg atggaaggcg gcggagagga ggagaaggaa cgagaggaac tgtcgaagga
     4381 caggtttctg gatctcgaac ggcatgctgg agctccttgg cgtcacgcca gatctctcct
     4441 gaacgaccct caagaaacga aatgatctcc cttgctcggc tgcagaccgg aagccgcgtc
     4501 tcggcacaga ggggctggtg gggaccgtgg agaagaggag gcgaggacga aggccatggg
     4561 cacgatcctc gaagagtgtc aggatcttag catgaaccct ccggcggggc ggtttcctga
     4621 gacgccctcg cggtgtctga ccagcagggg agagccaggc ctcccatgag taaaatagag
     4681 acgtacgagg agggactatg aacccgaagc actttctcgc actcttcctg ttgcccaccc
     4741 tcgcggcgcc ggccgccgcc caggtctccc ggggcgccga tccctgccta gaggcccggc
     4801 gcatcgccga atcccccgcc tgcctcgcgg gagccgccga ctgccaggcc gcgcagcgct
     4861 ggcagcaggt cttcggcacc ccgggaacgg gcctggccga tccgcagcgc acgggcgggc
     4921 tggccggtaa ggaggtccgc ctcgaggcta cggaggacct ccggagcgtc ctcacgcgcc
     4981 tggctgccgc ggccggcctg gagctgctca ccggcccgcg ggtcaccggc gagctccgcg
     5041 ccgaggccgg caccctccct ctgggggagg cctggcgcaa ggtcctggac acggcgggtc
     5101 tcgtggcgcg cttcgaggag ggcaaggtgc tcgtggccag ggccgatcgc gctcagggtg
     5161 gcactccccg gaggcagagg agcgccaccc tgttcaactg cccgacccgc tgagcggcgc
     5221 gcagcttact cgacagtcgg gccgcagaac tgccagaggc cgccggaccc ggcccaggag
     5281 gcccccgtcg ccgtggtgcc ggaagggcag ggggctccca gcaggctgca gcaccgcgac
     5341 ttggtggcgc tccaggtgac cgtggtgaag ccgctgccgc tgcagaaggt ccggcaggta
     5401 cctgtgtagg aggcgctggc gctcgtcagc gccgcgctgg cgagcacggc cgacaggctg
     5461 aggatgcgaa gcatcgattt catggtctct tctccttgca tgagccgcgg tgactggatg
     5521 gccatcgcgg cgagcgcccg cgggtcgcgg gcgctctcag gctctctggt gctccagggc
     5581 cccgcgatcg gccccccgct tccgggaaag ccgcagacgt gagaacctag tcagtccacg
     5641 agccgcctct ccctcgcgtt cgagggggga gacggcggca gagccgcggc ccactgcaag
     5701 ccaggtcgtc ccagaggccg ctccggaagg cgaggaggag aagacatggc caggaatggt
     5761 cgaacgacgc cgctcaactg gtacacgggc ggggttcccg atacggacgt caaggagacg
     5821 gaggagtggc tgaccgcctt cgagcaggtc atccagtacg agggtcgcga gcgggcgcag
     5881 ttcctgctca agaagctcct ggagaagggg tacgagaagg acgtcaccct cccgttcacc
     5941 ggcaacaccc cctacatcaa caccatcccg gcctccaagg agccctccta cggcggcgac
     6001 cgggcgatcg agcggcggat caagagcatc atgcgctgga acgccatggc gatggtggtg
     6061 cgggccaaca agaagcacaa cggcctgggc gggcacatct cgacgttcgc ctccgccgcc
     6121 accctctacc aggtgggcct caaccacttc tttcgcgggc gcggcgagag cggcttcgag
     6181 ggcgacatgc tctacatcca ggggcacgcc tcccccggca tctacgccca ggccttcctc
     6241 gaggggcgga tcaccgtcga gcagatggag gccttccggc gcgaggtgga ggtcaagggc
     6301 ggcctgtcgt cctatcccca cccctggctg atgccagact tctgggagtt ccccaccgtc
     6361 tccatgggcc tcggacccat caccgccatc taccaggcca agttcaaccg ctacctgcgc
     6421 gaccgcggcc tgcgcgaccc ccagaaccgg aagatctggg ccttcctcgg cgacggtgag
     6481 accgacgagc cggagaccct gggcgccatc acgctcgcgg cgcgcgagca gctcgacaac
     6541 ctcatcttcg tcatcaactg caacctgcag cgcctcgacg gcccggtgcg cggcaacggc
     6601 aaggtcatcc aggagctgga gaccgccttc cgcggcgccg gctggaacgt catcaaggtg
     6661 atctggggga gcgactggga tcccctcatc gaggcggaca acaccggact gctcatccag
     6721 accatgaacg agacgctgga cggcgagtac cagaagtacg tggtcgagcc cggctcctat
     6781 atccgtcagc acttcttcgg caagcacccc gagctgctga agctggtgga gggctactcc
     6841 gacgaggacc tccacgccct gcgccgcggc ggccacgacc cggtcaaggt ctacgccgcc
     6901 tacaaggccg ccgtcgagca cgagggatcg ccgacggtga tcctggcgaa gaccgtcaag
     6961 ggctacggca tgggcgaggc gggggagggg aggaacgtca cccatcagca gaagaagctc
     7021 aacgaggagg agctgcgctt cttccgtgac cgcttcgaga tcccggtctc cgacaaggac
     7081 ctggccgagg cgccgttcta ctgcccgccg gaggacagcg aggagatcca gtacctgcgc
     7141 gagcgccgcc gcgccctggg cggctacgtg cccagccgcc gcgtgcggaa ggagccgtcc
     7201 ccgatgccgg cggagagcac ctggggccgc ttctacgagg gggtggaccg cgacgtctcg
     7261 accaccatgg tcttcgtcca gatcctcacc cagctcatga aggacaagga gatcggcacc
     7321 cgggtggtgc ccatcgtgcc ggacgaggcc cgcaccttcg gcatggagtc cctgttccgc
     7381 cagttcggca tctactccca cgtgggccag ctctacgagc cggtggacaa ggggatgctc
     7441 ctctactacc gggaggccac ggacggccag atcctggagg aggggatcac cgaggccggc
     7501 tccatggcgt cgttcatcgc cgccggcacc tcgtacgcca cccacggcat cgacatgatg
     7561 ccgttcttca tcttctactc catgttcggc tttcagcggg tcgccgacct gatttgggcg
     7621 ggggccgatt cgcgcatgcg tggcttcctc cttggcgcga cagccggccg gacgacgc
//
LOCUS       DASPJI010000544         9224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_147633, whole genome shotgun sequence.
ACCESSION   DASPJI010000544 DASPJI010000000
VERSION     DASPJI010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9224)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9224
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147633"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VEL74_14940"
     CDS             <1..841
                     /locus_tag="VEL74_14940"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HYG63871.1"
                     /translation="DNAQGELYLTDAVTGAAAEGKPVRLLTLADPAEAWGVNTRAELA
                     LVHRRLVDRHLQALMVSGVTVLEPARTLIEPGVAVGPDTVIYPDVTLMGRTRIGSGCT
                     LHQGAWLRDTTVADGATIEPYSVLDGAEVGDGCRVGPFARLRPASRLLKGARVGNFVE
                     VKSSLLGEGSKAGHLAYIGDATIGEGANLGAGVVTCNYDGVRKHPTEIGRGAFIGSDT
                     MLVAPVKVGDGASTAAGSVVTKNVPEGALAVSRVRQKNLEGMAGGLRGSRHKGGAKDR
                     EET"
     gene            846..2675
                     /gene="glmS"
                     /locus_tag="VEL74_14945"
     CDS             846..2675
                     /gene="glmS"
                     /locus_tag="VEL74_14945"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HYG63872.1"
                     /translation="MCGIVGYVGSREVVPLLVDGLRRLEYRGYDSAGVVAVQEGTLET
                     RKAEGKLDRLVEKLAQQPLSGHYGLGHTRWATHGPPSERNAHPMVDSKRRLALIHNGI
                     IENFLPLKKRLIAAGWTFTSDTDTEVVANLISSYLDGDLRSAVARAVRELEGMYAFAV
                     VTVENEGQEIVAARQGPPLVIGLAQDEQFLASDPAALLVHTKDVIFLENGDLACLTPR
                     KVEVWDRAGLPVERPVQRLNWDPIQTEKGGYKHFMLKEIHEQPQAVQDTFGARVDFEG
                     GHVLFDTVKIPDADLAKIERVQMLACGTSWHSGLIGKFLIEHIARLPVEVDYGSEYRY
                     RDPIASDRVLAVGISQSGETADTLSAMEAAHERGARLFTVCNVPGSQATRISEGVIYT
                     HAGPEIGVASTKAFTTQLVALYLLALYLRQVKDGGVDRRLLDDLARLPQAVNDVILLQ
                     EKPMEEMARKYFRSENFLYLGRGINYPIALEGALKLKEISYIHAEGYPAGEMKHGPIA
                     LIDSEMPVVAIATGNDTVFEKVKSNLQEVNARDGIVIAVTDRDPEELGGICDEIIEVP
                     SVSPLLQPIVNVIPLQLLAYFTGVRRGADVDQPRNLAKSVTVE"
     gene            2672..4540
                     /locus_tag="VEL74_14950"
     CDS             2672..4540
                     /locus_tag="VEL74_14950"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HYG63873.1"
                     /translation="MSAETSRAPVNVYQERAAAFTEEERRRSRVSFRFSLLRGGLFFA
                     FAGCLLMILITAGTFVPGWWIGAGAALLGFLAVLPVHDRVIAAEHRARDLRQINEEGG
                     RRLARDWDGLPLPTFSGPGVEDREDLPLARDLNLFGRASLSHLLGTAHTPPGKATLAG
                     WLVHPAPPEEIVRRQEAVAELAPALELRQQLQVRARPMDRVPPNVEPFLRWAEDEPWL
                     LGRPWLVWLARALAVLTLGLVVAAIATPLPSWPALLMITVNLLLSYGLQGRLEAVFTR
                     VSAREGEFLLYAEALRVIDEAPVQAAALRQIHEELEPRGMPAHRWLDLLHRRVTLADS
                     RHSALVHTPLQLLTLWDFHVVWLLERWQRDAGPHARRWLAALGRFEALSALAGLRFDH
                     PGWACPSVVAGEPSLTARALGHPLIADAHRVTNDVELGPPGTFLLVTGSNMSGKSTLL
                     RSIGVNVVLAQAGGPVCAEAMRLPPVDLGTSILVEDSLADGVSFFMAELLRIRRIVAA
                     ADRSHAQGRAFLYLLDEILRGTNSYERQVAVRKVLLHLLRQGAIGAVSTHDLELAELQ
                     ELQASCRPVHFREDFHRDAQGKPLMTFDYKMRPGVATTTNALKLLELVGLSDLDTR"
     gene            4655..4978
                     /locus_tag="VEL74_14955"
     CDS             4655..4978
                     /locus_tag="VEL74_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63874.1"
                     /translation="MAEPARKALNPEDAASAELIDDEAIPGIDIVQRWVEGPDGRMEL
                     LELPLTPELFLDPQDEDKIIQHPDHHKAVHALFDRLDLHFESDPDVMVLSDAKHLLGP
                     GAPAG"
     gene            5061..5315
                     /locus_tag="VEL74_14960"
     CDS             5061..5315
                     /locus_tag="VEL74_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017302727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYG63875.1"
                     /translation="MNAVTLKEAELKLKELVEQVIADAEPTIVVTESGDQVVFLPLDE
                     YNSWKETVYLLSSPANAEHLHRSITEAREGNVQERELLDA"
     gene            5312..5572
                     /locus_tag="VEL74_14965"
     CDS             5312..5572
                     /locus_tag="VEL74_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017606738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HYG63876.1"
                     /translation="MKLGFTGPAWEDYLWFQEHDRKLLKRINLLIQDVLRTALEGIGK
                     PEPLKAHLKGFWSRRINDEHRLVYTATATEVTIIACRYHYSK"
     gene            complement(5707..6513)
                     /locus_tag="VEL74_14970"
     CDS             complement(5707..6513)
                     /locus_tag="VEL74_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lnu(F)/Lnu(G) family lincosamide
                     nucleotidyltransferase"
                     /protein_id="HYG63877.1"
                     /translation="MLQQRMIDRVRELCHVDERLVGALMFGSFTIGEGDAFSDIEFAV
                     FIRDEDFQGFEQRAWLDAVSPVAGYFVDDFGHHTALFENGVRGEFHFLRASDVPIVSG
                     WQGHGWFPSLDAAVVLDRTGELSRHARVLVGGPPERGGAPLVEGLALNLINLMLLGAN
                     LLNRGEFARAWALLGKAHESLLKLVRLQEGATDHWPTPSRRLEKDLSAASYERYLTCT
                     ASAQPGVLRAAYRESWTWSRELFETVAEPLDVELPATVIERVGELLDEAL"
     gene            complement(6590..6718)
                     /locus_tag="VEL74_14975"
     CDS             complement(6590..6718)
                     /locus_tag="VEL74_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63878.1"
                     /translation="MTLNAEALQEAHGAATYRSDCNCPILSIGQCATFKWTNCTNC"
     gene            complement(6913..7422)
                     /locus_tag="VEL74_14980"
     CDS             complement(6913..7422)
                     /locus_tag="VEL74_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63879.1"
                     /translation="MSGVSQTPNAFAARFLSRLGERDEPPTAGEAAVAGPWRIEQLPG
                     RGFGLFRAGKGVARGGRPAAICPSRWVALAFAAMLPGTGRDPMLRLGKEADGEGMYPV
                     TLDTGEVVARLEVFDDTLIERVNALIHLLQSPQSLADALEAAGAEALDACGSLLDERI
                     APPEATPLR"
     gene            complement(7636..7821)
                     /locus_tag="VEL74_14985"
     CDS             complement(7636..7821)
                     /locus_tag="VEL74_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63880.1"
                     /translation="METISSGMKAVPRRMVSIPGWWTAIPGEMTRFWEDSLPKLLLGR
                     RRRAEAEKTLAFCLVLF"
     gene            8012..8599
                     /locus_tag="VEL74_14990"
     CDS             8012..8599
                     /locus_tag="VEL74_14990"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HYG63881.1"
                     /translation="MERRPADGIESGDAEGAPPLDRRPESLLVPGLGQLYAGEPKRGI
                     ALLLGHFGIVALLPAVGLLRTFAGLAVFLTGFLLAQVWIVWDAARAARRNRSYVLRPF
                     NRWYVYLAAFLVVNLLITPRITTFWATRSFSIPSGAMEPALLIGDHLIADMKWYGSRR
                     PERGDLVALRGRALYVYWAADKSRIGTSLLTGDRR"
     gene            complement(8580..9074)
                     /locus_tag="VEL74_14995"
     CDS             complement(8580..9074)
                     /locus_tag="VEL74_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYG63882.1"
                     /translation="MTETAPVVKVQMLIRRPVAQVFEAFVDPAVTSRFWFSRGSGRLE
                     AGRRVRWDWEMYGASAEVDVKEIEENRRILIEWDGPDHPTQVEWTFEPRGEDRTYVTV
                     RNWGFHGTPDEVVARALDSTGGFTFLLSGAKVFLEHGIEPKLVEDHVPDALVEGWAPS
                     GDRR"
BASE COUNT         1510 a         3242 c         3027 g         1445 t
ORIGIN      
        1 cgacaacgcc cagggagagc tctacctgac cgacgcggtt accggcgccg cggcggaggg
       61 gaagccggtc cgcctgctca cgctcgccga cccggccgag gcctgggggg tcaacacccg
      121 cgccgaattg gcactcgtcc accgccggct cgtcgaccgc cacctccagg cgctcatggt
      181 ctcgggggtg acggtgctgg agccggcgcg caccctgatc gagcccggcg tcgccgtcgg
      241 gccggacacg gtgatctatc cggacgtgac cctgatgggc cgcaccagga tcggtagcgg
      301 ctgcacgctc catcagggag cctggctgcg cgacaccacg gtggcggacg gcgccaccat
      361 cgagccctac tcggtgctcg acggcgccga ggtgggggac ggctgccggg tgggcccgtt
      421 cgcccgcctg cgcccggcat cccgcttgct gaagggagcg cgagtgggaa acttcgtcga
      481 ggtgaagagc tcgctcctcg gagaggggag caaggccggc catctcgcct acatcgggga
      541 cgccaccatc ggcgaggggg cgaacctcgg cgccggggtg gtcacctgca actacgacgg
      601 cgtccgcaag catccgacgg agatcgggcg cggcgccttc atcggaagcg ataccatgct
      661 ggtggcgccg gtcaaggtgg gcgatggcgc gtccaccgcc gccggctccg tcgtcaccaa
      721 gaacgtaccc gagggtgccc tggccgtgag ccgggtgcgc cagaagaacc tcgaagggat
      781 ggccggcggg ctgcgcggct ctcgtcacaa gggcggcgcc aaagaccggg aggagacctg
      841 aagctatgtg cggaatcgtc ggttacgtag ggagccgcga ggtggtcccc ctgctggtgg
      901 acggcctgcg gcggctggag taccgggggt acgactcggc cggcgtcgtc gccgtccagg
      961 aggggacgct cgagacgcgc aaggcggagg gcaagctcga ccgcctggtc gagaagctgg
     1021 cgcagcagcc gctgtccggc cactacgggc tcggccacac ccgctgggcc acgcacggcc
     1081 cgcccagcga gcgcaacgcc caccccatgg tggactccaa gcgccgcctg gcgctgatcc
     1141 acaacggcat catcgagaac ttcctgccgc tcaagaagcg gctgatcgcc gcggggtgga
     1201 ccttcacctc cgacaccgac accgaggtgg tggcgaacct catctcctcc tacctggacg
     1261 gcgacctgcg gagcgccgtc gcccgcgccg tccgcgagct cgaggggatg tacgccttcg
     1321 ccgtggtcac cgtggagaac gagggacagg agatcgtcgc cgcccgccag ggcccgccgc
     1381 tggtcatcgg cctcgcccag gacgagcagt tcctcgcctc cgatccggcg gcgctcctcg
     1441 tccacaccaa ggacgtgatc ttcctcgaga acggcgacct tgcctgcctg acgccgcgga
     1501 aggtcgaggt ctgggaccgc gccggcctgc cggtcgaacg gccggtccag cgcctcaact
     1561 gggacccgat ccagaccgag aagggcggct acaagcactt catgctcaag gagatccacg
     1621 agcagccgca ggcggtccag gacaccttcg gcgcgcgcgt cgacttcgag ggcggccacg
     1681 tcctcttcga cacggtgaag atcccggacg ccgacctggc gaagatcgag cgcgttcaga
     1741 tgctggcctg tggcacctcc tggcactcgg ggctgatcgg caagttcctg atcgagcaca
     1801 tcgcccgcct gcccgtcgag gtggactacg gctccgagta ccggtaccgc gacccgatcg
     1861 cgagcgaccg cgtgctggcc gtcggcatct cgcagtcggg tgagaccgcc gacaccctct
     1921 ccgccatgga ggccgcccac gagcgggggg cgcgcctgtt cacggtctgc aacgtgccgg
     1981 gcagccaggc gacgcggatc agcgaggggg tgatctacac ccacgccggc ccggagatcg
     2041 gcgtcgcctc gacgaaggcg ttcaccaccc agctcgtcgc cctctacctg ctcgccctct
     2101 acctgcgcca ggtgaaggac ggcggggtgg accgccgcct gctcgacgac ctggcgcggc
     2161 tgccgcaggc ggtcaacgac gtcatcctcc tgcaggagaa gccgatggag gagatggcca
     2221 ggaagtactt ccggtcggag aacttcctct acctcggccg cggcatcaac tatcccatcg
     2281 cgctcgaagg ggcgttgaag ctcaaggaga tctcctacat ccacgccgag ggctacccgg
     2341 cgggcgagat gaagcacggc cccatcgccc tgatcgacag cgagatgccg gtggtggcga
     2401 tcgccacggg caacgacacc gtcttcgaga aggtgaagag caacctgcag gaggtcaacg
     2461 cccgcgacgg catcgtcatc gccgtcaccg accgcgaccc cgaggagctg gggggcatct
     2521 gcgacgagat catcgaggtg ccgtcggtgt cgccgctgct ccagccgatc gtcaacgtca
     2581 tcccgctcca gctcctcgcc tacttcaccg gcgtgcgccg cggcgcggac gtcgaccagc
     2641 cgcgcaacct ggccaagagc gtcacggtgg agtgagcgcc gagacgagcc gggcccctgt
     2701 caacgtctac caggaacgcg ccgccgcctt caccgaggag gagcggcggc gctcccgggt
     2761 ctctttccgt ttctccctgc tgcgcggcgg cctcttcttc gccttcgccg gctgcctgct
     2821 gatgatcctg atcacggccg ggaccttcgt ccccggctgg tggatcggcg ccggcgccgc
     2881 gctgctcggc ttcctcgcgg tcctgccggt gcacgacagg gtaatcgccg ccgagcaccg
     2941 cgcccgcgac ctgcggcaga tcaacgagga gggggggcgg cggctcgccc gcgactggga
     3001 cgggctgccc ctgccaacgt tctcggggcc cggggtcgag gaccgcgagg acctccccct
     3061 cgcccgcgac ctgaacctct tcggccgcgc ctccctctcg cacctcctgg ggaccgccca
     3121 cacgccgccc ggcaaggcga ccctcgccgg ctggctcgtc caccccgcgc cgccggagga
     3181 gatcgtccgg cgccaggagg cggtggcgga gctcgccccg gcgctcgagc tgcggcagca
     3241 gctccaggtg cgcgcccgcc ccatggaccg ggtgcccccc aacgtcgagc cgttcctgcg
     3301 ctgggccgag gacgagccct ggctgctggg ccgcccctgg ctggtctggc tcgcccgcgc
     3361 cctcgccgtc ctgaccctcg gcctggtggt cgccgccatc gccacgccgc tcccctcctg
     3421 gccggcgctg ctcatgatca cggtgaacct gctgctgagc tacgggctcc aggggcgtct
     3481 cgaagcggtc ttcacccgcg tctcggcgcg cgagggggag ttcctcctct acgccgaggc
     3541 cctgcgggtg atcgacgagg cgcccgtgca ggccgcggcg ctgcgccaga tccacgagga
     3601 gctggagccc cgggggatgc cggcgcaccg ctggctggac ctgctccacc gtcgcgtgac
     3661 gctcgccgac tcgcgccact cggccctggt ccacaccccg ctccagctcc tgacgctctg
     3721 ggacttccac gtcgtatggc tgctggagcg ctggcagcgg gacgccggcc cgcacgcccg
     3781 ccgctggctg gcggccctcg gccggttcga agcgctctcg gccctggccg gcctgcgctt
     3841 cgaccacccc ggctgggcct gcccgtcggt cgtggcgggg gagccgagcc tgaccgcccg
     3901 cgccctgggg catcccctca tcgccgacgc gcatagggtg accaacgacg tggagctggg
     3961 cccgccgggc acgttcctgc tggtgaccgg ctccaacatg tcgggcaaga gcacgctgct
     4021 gcggagcatc ggggtgaacg tcgtcctcgc ccaggccgga ggcccggtct gcgccgaggc
     4081 gatgcgtctc cccccggtgg acctcggcac cagcatcctg gtcgaggact ccctggccga
     4141 cggcgtctcc ttcttcatgg ccgagctgct gcgcatccgc cgcatcgtgg ccgcggccga
     4201 ccgcagccac gcccagggga gggccttcct ctacctgctc gacgagatcc tgcgcggcac
     4261 caacagctac gaacggcagg tcgccgtccg caaggtcctc ctccatctct tgaggcaggg
     4321 ggcgatcggc gcggtctcga ctcacgacct ggagctggcg gagctgcagg agctccaggc
     4381 ctcctgccgt ccggtccact tccgggagga cttccaccgc gacgcccagg ggaagccctt
     4441 gatgaccttc gactacaaga tgcgccccgg cgtcgcgacc accaccaacg ccctgaagct
     4501 gctggagctg gtggggttga gcgacctgga cacacgctga gcctatcctg cgaggcccgg
     4561 ttacgccatt ccgggtcctc tctcatccga atctccgtga tacgggaccc cgggactctt
     4621 tgacgggtcc cgggaggtct tctatactcg catcatggcc gagcccgcga ggaaagccct
     4681 gaaccctgag gatgccgcgt cggcggagct gatcgacgac gaggcgatcc ccgggatcga
     4741 catcgtccag cgatgggtcg agggccccga tggccgcatg gagctgctgg agctccctct
     4801 caccccggag ctgtttctcg acccgcagga cgaggacaag atcattcagc accccgacca
     4861 ccacaaggcg gtccacgccc tcttcgaccg gctggacctc cacttcgaat ccgatcccga
     4921 cgtgatggtg ttgtccgatg ccaagcatct cctgggcccc ggcgcgcccg ccggctaaaa
     4981 aaggcaccac ggccgatcgt gctatcctga cattctgttg gagtgggggc ttcaagagca
     5041 gtacgtgaga ggatgggttc atgaacgcgg tgacgttgaa ggaagccgag ctgaagctga
     5101 aagagctggt cgaacaggtg atcgcggatg cggagccgac catcgtggtc accgagagcg
     5161 gcgaccaggt cgtgtttctg ccgcttgatg agtacaactc ctggaaggag acagtctact
     5221 tgctttccag ccccgccaac gccgagcacc tgcatcgctc gatcaccgag gcacgggaag
     5281 gcaacgtcca ggagcgcgag cttctcgacg catgaaactc ggcttcacag gcccggcgtg
     5341 ggaggactat ctctggttcc aggagcacga ccggaagctc ctgaagcgca tcaacttgct
     5401 aatccaggac gtcctccgta ccgcccttga ggggatcggt aagccggagc cgctcaaggc
     5461 tcacctgaag ggtttctggt cccgccgcat caatgacgaa catcggttgg tgtatacggc
     5521 aaccgccacc gaggtcacga tcatcgcctg ccgctatcac tactcgaaat agaaagtagc
     5581 agggccggat cacctttgcc ggaggctggc ccaggggtca tgccgcgtca ttcaggacct
     5641 cagcttccga caagacctca tcccggaagt aggggctcag gaaaaatgag gaaaggtgct
     5701 cggagctcac agggcctcat ccagcagctc acccacccgc tcgatgaccg ttgccggcag
     5761 ctcgacatcc agaggttcgg cgactgtttc gaacaactcg cggctccacg tccacgactc
     5821 gcgataggcg gcgcggagaa cgcccggctg cgcgctggcc gtacaggtca gataacgctc
     5881 ataggaggcg gcggagagat ccttctccag cctccgtgaa ggggtgggcc agtggtccgt
     5941 cgcgccttcc tggaggcgaa ccagcttgag caagctttcg tgggccttgc cgagcagggc
     6001 ccaggcccgg gcaaactcac cgcgattcag aaggttcgct ccgaggagca tcaggttgat
     6061 gaggttcagg gcgagcccct ccaccaaggg agcgcctcca cgctccggag gcccgcccac
     6121 gagcacacgc gcgtgccgtg acagctcgcc ggtccggtcc agcacgacgg ccgcgtcgag
     6181 cgaggggaac cacccatggc cttgccagcc ggaaacgatc gggacatcgg aggcacgcag
     6241 aaagtggaac tcgccgcgca cgccattctc gaaaagcgcc gtgtgatggc cgaagtcatc
     6301 cacgaagtaa ccggccaccg ggctgacggc gtcgagccag gcacgctgct cgaagccctg
     6361 gaagtcttca tcacggatga acacggcaaa ctcgatgtcg gagaaggcat ccccctcccc
     6421 aatcgtgaag gagccgaaca tcagcgcgcc gacgaggcgt tcgtcgacat gacaaagctc
     6481 gcgaacgcgg tcgatcattc tctgctgaag catgtttcct ctgctctgac gatgggaggg
     6541 aaccggcggt gagcggcaga atccgcccaa ccgcctgctc ctcaggtcgt cagcagttgg
     6601 tgcagttggt ccacttgaag gtagcgcact ggccgatgga aaggatcggg cagttgcagt
     6661 cgctccggta ggtggcggcg ccatgtgcct cctggagcgc ttcggcattg agcgtcaaga
     6721 gcgtttcacg atgaagcgtc aactttcggg gctgtttctt cgtcattctg acctcctagt
     6781 caggcttctc ggctcattcc ggaagcttct cgggcccctc cggaagcttc cgaagcggct
     6841 cttcgagtct cattttggga agaacagcgc cgtggcctgc ggagcaggcc acggcttctc
     6901 tacttcttca gcttaccgga gcggcgtggc ctccggcggg gcgatcctct cgtcgaggag
     6961 gctcccgcag gcgtcgagag cctcggctcc cgcggcctcc agggcgtccg ccagggactg
     7021 cggggactgc aggagatgga tgagggcgtt gacgcgctcg atcagggtgt cgtcgaagac
     7081 ttcgagccgc gccaccacct cgccggtgtc cagggtgacc ggatacatcc cctccccgtc
     7141 cgcctccttg ccgagccgga gcatgggatc gcggccggtg ccggggagca tcgcggcgaa
     7201 ggccagggcg acccaacgcg acggacagat cgccgcggga cgccctcccc gggcgacacc
     7261 cttgcccgcc cggaagaggc cgaaacctct cccggggagc tgctcgatcc gccacggacc
     7321 cgccaccgcg gcctcgccgg ccgtcggcgg ctcgtccctc tcgcccagac gggagagaaa
     7381 ccgggccgcg aacgcgttgg gagtctggga aacgccggac acactggtac tatcgtggga
     7441 accacacata aggaacaacc ctccttgtgt ctcgtggcca ggcccggtcc gtgagtgtca
     7501 gctcacttcc gggccgactc ggcagcattg ccgggccacg tcctgccttg tacgacgggt
     7561 cgactcccac accggagagc tttcgcggtg ccgcgcgccc cccggcgtgt cctgctacaa
     7621 cgtgatgagc caatcctaga agaggaccag gcagaaagca agcgtcttct ccgcctcggc
     7681 gcggcggcgc cttcccagga gcagcttcgg caaggaatct tcccagaagc gcgtcatttc
     7741 tccaggaatg gccgtccacc acccgggaat ggacaccatc cttcggggaa cggccttcat
     7801 tcctgaagaa atggtctcca tgaaacggcc ttcattcctg aagaaacgcc cgccattcct
     7861 tcagaaatta ggatcatttc gggagaaatg gccgtcattc cccagggaac ggagaccatt
     7921 tccgagggaa cggcgttcat ttccccgaga atgacgatga tttctggaga aatggcgcag
     7981 gcccgaggac cagcaggagt gggatatcct ggtggaaagg agacctgccg atgggatcga
     8041 gtccggagac gccgaaggcg cgccgcccct ggatcgccgt cctgagtccc tgctggtgcc
     8101 ggggctcggg cagctctatg ccggcgagcc aaagcggggg atcgccctcc tcctcggcca
     8161 cttcgggatc gtcgccctcc tcccggcggt gggcctgttg agaaccttcg cgggactcgc
     8221 ggtcttcctc accgggttcc tgctggccca ggtctggatc gtctgggacg cggcccgcgc
     8281 cgcccggcgc aacaggagct acgtcctccg gccgttcaat cgctggtacg tctatctcgc
     8341 cgcgttcctg gtagtgaacc tgctgatcac cccgcggatc accaccttct gggccacccg
     8401 gtccttcagc atcccttccg gagccatgga gccggcgctc ctcatcggcg accacctcat
     8461 cgcggacatg aagtggtacg gctcccgccg gccggagcgc ggagacctcg tcgccctcag
     8521 aggccgggcg ctctacgtct attgggccgc cgacaagtcg aggatcggca cttccctgct
     8581 caccggcgat cgccgctagg agcccacccc tcgacgagcg cgtccggcac gtggtcctcg
     8641 acgagcttcg gctcgatccc gtgctccagg aagaccttcg cgccggagag caggaaggtg
     8701 aagccgccgg tggagtcgag cgcccgcgcc acgacctcgt ccggggtacc gtggaagccc
     8761 cagttcctga cggtgacgta ggtccggtcc tcgccgcggg gctcgaaggt ccactcgacc
     8821 tgggtgggat ggtcggggcc gtcccactcg atgaggatgc gccggttctc ctcgatctcc
     8881 ttcacgtcga cctcggcgga ggcgccgtac atctcccagt cccagcggac ccgccgcccc
     8941 gcctccagcc ggccgctccc cctggagaac cagaagcggg aggtgacggc ggggtcgacg
     9001 aacgcctcga atacctgcgc gaccggccgg cggatgagca tctgcacctt cactaccggg
     9061 gctgtctctg tcatgtcggc ctcctcccct gagaggcgcc gattctatcc cgcgtacgcc
     9121 cgctcgatct cggcgatgtc gatcttcttc atctgcagca tcgccgtcat ggctctctgg
     9181 gaccctttgt tctcggggtc gccgaacagc tcgttcagga gcac
//
LOCUS       DASPJI010000545         9096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_547823, whole genome shotgun sequence.
ACCESSION   DASPJI010000545 DASPJI010000000
VERSION     DASPJI010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9096)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547823"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VEL74_15000"
     CDS             complement(<1..882)
                     /locus_tag="VEL74_15000"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63883.1"
                     /translation="MILRQGWAGAAAALHSPAMWSDRLPEGRMRKMVERLRGVLPAPA
                     PGSRRSRTPLRAAADPLLVAERLRGEPGFSWLDGGDPGGNPGGNHRLYFEPVATLSAA
                     KGRCLVTGPGGSAGFKAAAFDLLAAAFAAWQGDGGVLVGFLGYELGGELEPLPAPPPD
                     DLGAPDLHLHLYDAALVGDGRAWTLEATDSWGGPARPMERVLQAERLLAEAAQLSPPE
                     VPSGRLAAGPVASRPSRGGFEAAVRRAVERIGTGEIFQVNLCRRLEASLPAHLVWPFH
                     LRLRAASPAEHGAYFDLS"
     gene            904..1659
                     /locus_tag="VEL74_15005"
     CDS             904..1659
                     /locus_tag="VEL74_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HYG63884.1"
                     /translation="MDQNINRRRFLGLGLTGAAALLAGPRLAWPAEGSGAARLESRPG
                     KPTQPGQVGIHKLGLAKGRDGFLSIPKSYKPEKPVPLMVMLHGARGRTDGFNAFAVGA
                     AAMGIAVMAPDSRGSTWDSILGGFGPDIAFLDRALRHTFARLAVDPRRVALAGFSDGA
                     SYALSAGLANGDLFSHLVAFSPGFMSPPGRQGKPAIWISHGRSDEVLPVDLSRRIVPR
                     LKQWGYKVQYREFDGGHTVTPELGTEAFRWFVA"
     gene            2203..2439
                     /gene="vapB"
                     /locus_tag="VEL74_15010"
     CDS             2203..2439
                     /gene="vapB"
                     /locus_tag="VEL74_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HYG63885.1"
                     /translation="MPPMIARIFRNGRNQAVRIPVELSLDAETVTIERQGDALILRPV
                     ASGGWDQFFADPSLVLPPEFELEDDPPPQERHVF"
     gene            2439..2873
                     /locus_tag="VEL74_15015"
     CDS             2439..2873
                     /locus_tag="VEL74_15015"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG63886.1"
                     /translation="MYVLDTDICSYLMKRLHPALIERVREFAPRELKVSVITLFELEY
                     GILRSERRDPLRRVVRAFLENVEILDWTPSAAREAGAVRSELATAGRPIGAYDLLIAG
                     HVRSLDATLVSHNVREFSRVPGLRLADWTGGAEPSHPQAGTP"
     gene            3137..3637
                     /locus_tag="VEL74_15020"
     CDS             3137..3637
                     /locus_tag="VEL74_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63887.1"
                     /translation="MSHPANVFTAEFLARLGERDEPPTAGEAAVAGPWRIEPVPGRGF
                     GLFRAGQGLARGHRPVAVCPSRWAALVTAAVLPGTGRDPLLRLSKEPDTDGMYPVTLD
                     TGEAVMHLKYFDETLVDHANALIHLLRSPSSLADALEAGGAEALGLCGSLLDERFPPS
                     EDLPSR"
     gene            4086..5042
                     /locus_tag="VEL74_15025"
     CDS             4086..5042
                     /locus_tag="VEL74_15025"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HYG63888.1"
                     /translation="MKGPRRTLSLVTVLLLAAGTALSYTLTSPQRTWNCPTTYVVDQS
                     GLPSIADADGGATAVVQAITSSNAWNGAGAGRIIKASKGDVSGLTSGDGIPMISFSDP
                     LGICTGSCIAGSLNGYSLRSPGSGSYKLTDVDLVFNPSSSTSWTSQGEDPGGAGCSGE
                     VYIEGFTVHEVGHGLGLGHTAVAGATMYPSVPSCSNSQATTEADDEAGLRALYGTSPC
                     PACNSHTEYLASLEQEIQPCGSAYKTSVTGFHEGWLEGPATSNFDLYLDRYNGLAWVV
                     VASSTGAASSEAISYNGLPGRYRYRVVAASGTGTYHFWERHP"
     gene            5933..6382
                     /locus_tag="VEL74_15030"
     CDS             5933..6382
                     /locus_tag="VEL74_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HYG63889.1"
                     /translation="MMRSERLSVALHVLLHMAERPEEPMTSQEMAECVGTNPVVIRRT
                     FAGLREAGIVSSVKGHGGGWRLARPASSVNIAEIQRALGEQVVSFTSTVEPPRCVLLK
                     AVIHSLDEAVKEAEQVLERRLSTLTLADLASSVTGSHVRPGGRPRAK"
     gene            6372..7268
                     /locus_tag="VEL74_15035"
     CDS             6372..7268
                     /locus_tag="VEL74_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYG63890.1"
                     /translation="MQNDAIVVGGSFAGLSAAMQLARARKRVLVIDAGQPRNRFAKTS
                     HGFFGQDGKPPAEILREAARQVAAYPTVRFIQGEATGASQTDGGFTVTLGDGREEQAA
                     RLVLATGVRDELPPIPGMRERWGRTVLHCPYCHGYEVAGGALGVLASDPVMAVHKAML
                     IPDWGQTTLFTQGLFEPDAEQAAQLAARGVQVERAPVVELLGEAPELEAVRLADGRVI
                     PLQAIFAAPDVRMASPLAEALGCAFEQGPQGPYIRVDDRKQTTVAGVYAAGDASAAMH
                     NATLASASGVLAGIGAHQSLAK"
     gene            complement(7318..8508)
                     /locus_tag="VEL74_15040"
     CDS             complement(7318..8508)
                     /locus_tag="VEL74_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63891.1"
                     /translation="MLKAMVAVLALGGLPVAGTLEAQKIANWPVASATWSPEAEAAGE
                     KAVPTPPLRFTGITPCRIADTRRSTLPAGYGLPKLAPGTPRTLVMIGQAHCSVPATAQ
                     AVSVNVTITQADGLGDLVFYPAGGVRPNTSTINYEPGQNLANAAILPLGNSGALNVEA
                     EFSAVHMILDINGYFAADDSQLVWRGAWNPSVSYNVNDLVSYNGSSYIGRAPNIGVTP
                     SSGLPNWDLLAQGGTSAGGPWQVSGSNIYYSGGNVGIGTSSPSDKLEIRQGNILLDAT
                     YGLLSTGGARIQTGYLGGTFSFHAGSSASSIAHFFDHNGVLKLVVDNNGVSFTGQKLC
                     SAYSTTPWWRDTIVVSSTWTAATCLNFRNAVGANFYQLGCQFQDNFSFGTANGGAPPV
                     NCGW"
     gene            8685..>9096
                     /locus_tag="VEL74_15045"
     CDS             8685..>9096
                     /locus_tag="VEL74_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020476571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYG63892.1"
                     /translation="MTIPTETSLFDGLQAARELAAEQERLRITLASIGDAVISTDAEG
                     RVTYLNGVAEALTGWTQAEAAGRPLLEIFQILNEETRQPAENPAAQALREGVVVGLAN
                     HTVLIARDGTERPIDDSAAPMLDSSGRPVGVVLVF"
BASE COUNT         1418 a         3073 c         3155 g         1450 t
ORIGIN      
        1 ggagagatcg aaataggcgc catgctcggc cgggctcgcg gcgcgcagcc gcagatggaa
       61 cggccagacg agatgggcgg ggagggaggc ctcgagccgg cggcagaggt tcacctggaa
      121 gatctcgccg gtgccgatgc gctccacggc gcggcggacc gcggcctcga acccgccccg
      181 gctgggacgg ctcgccaccg gccccgccgc caggcggccg gacggcacct ccggcgggga
      241 gagctgcgcc gcctcggcga gcaggcgctc ggcctggagc acccgctcca tcggccgcgc
      301 cggtccgccc caggagtccg tggcctccag cgtccacgcg cggccgtccc cgaccagcgc
      361 cgcgtcatag agatgaaggt ggaggtcggg ggcgcccagg tcgtccggcg ggggagcggg
      421 gaggggctcc agctcgccgc cgagctcgta gccgaggaag cccaccagga cgccgccgtc
      481 gccctgccag gcggcgaagg cggcggcgag gaggtcgaag gcggccgcct tgaagccggc
      541 gctgccgccc ggcccggtca ccaggcaccg ccccttggcg gcggacagcg tggcgacggg
      601 ctcgaaatag agccgatggt tgccgccggg gttgccgccg gggtcgccgc cgtcgagcca
      661 ggagaagccg ggctcgcccc gcagccgctc ggccacgagg agagggtccg ccgccgcccg
      721 caacggcgtc ctggaccgcc gggagcccgg ggccggcgcc ggcaggactc cgcgcaggcg
      781 ctccaccatc ttccgcatcc gcccttcggg gagccggtcg gaccacatgg ccggagagtg
      841 tagcgcggcg gcggcgccgg cccagccctg gcgtagaatc accgcctctc tggaggagcg
      901 gtcatggatc agaacatcaa ccgccggcgc ttcctgggtc tgggcctgac cggcgccgcc
      961 gcgctgctcg ccggcccgcg tctcgcctgg cccgccgagg gctcgggggc ggcccgcctc
     1021 gagtcgcggc cgggcaagcc cacacagccg ggccaggtgg gcatccacaa gctcggcctc
     1081 gccaaggggc gggacggctt cctctccatc cccaagagct acaagccgga gaagcccgtc
     1141 cccctgatgg tgatgctgca cggcgcccgg gggcggacgg acggcttcaa cgccttcgcc
     1201 gtcggcgccg ccgcgatggg gatcgcggtg atggcgccgg actcgcgcgg ttccacctgg
     1261 gactccatcc tcggcggctt cggaccggac atcgccttcc tcgaccgcgc cctgcggcac
     1321 accttcgcgc gcctggcggt cgacccgcgg cgtgtcgccc tggccggctt ctcggacggc
     1381 gcctcctatg ccctctcggc cggcttggcg aacggcgacc tcttctcgca cctggtcgcc
     1441 ttctcgcccg gcttcatgag cccgccgggg cgccagggca agccggcgat ctggatctcg
     1501 cacggcaggt ccgacgaggt cctgccggta gacctgagcc gccggatcgt cccgcgcctc
     1561 aagcagtggg gctacaaggt gcagtaccgg gagttcgacg gcggccacac cgtcaccccg
     1621 gagctgggca ccgaagcctt ccgctggttc gtggcgtagc gggaagcctc gaaacggtcc
     1681 acgggcgcgc cgccgccgcc ttccatcggc ggccgcgccc tggaacctgg tccatcgatt
     1741 gtcaaagaac ctccgcgagc cgtcccgcgc ggttggaccc gcgccggacc tgtctcgacc
     1801 ctcctccaat cccgtccgac cctcttccga gcgcccgcga cccgagggtc gagaggcgcc
     1861 gcgctcttca aacccgcttc gacccggctc aaacctctcg cgacccgagg gtcgagagtc
     1921 tcgccggtcg ggtcggatcg ctgttgagcc gcgatccttc cacctcatga gagcgcatcc
     1981 gtccaggaaa gtcaaattcg gtcttgtcag ccgaaccggt tccatgtaga gtgagatgga
     2041 gaccggcgct gcccgccggc agggtgttcc ttgaaaactg aatccggcag cgggcggagg
     2101 tccgatccct ccgccggctg ccgtatggcc ggagcccggc gtgcttgacg ggccttctcg
     2161 atgggcctat gctgacgaca taggctagag ggaggggacg tcatgccacc gatgattgca
     2221 aggatctttc gcaacggccg gaaccaggcg gtccgtatcc cggtcgagct gagtctggat
     2281 gcggagaccg tgaccatcga acggcaagga gacgccttga tcctccgccc cgtggcctca
     2341 gggggttggg accagttttt tgccgacccc tcgctcgtgc tgccgccgga gttcgagctg
     2401 gaagacgatc cgccccccca ggagcgccat gtcttctgat gtacgtcctc gatactgaca
     2461 tctgcagcta tctcatgaag cgccttcatc cggcgctgat cgagcgtgtg agggagttcg
     2521 cgccgcggga gctcaaggtc tccgtcatca cgctctttga gctggagtac gggattctgc
     2581 ggagcgagcg tcgcgacccc ctgcgccgcg tcgtgcgggc cttcctcgag aacgtcgaga
     2641 tcctggattg gactccctcc gcggcgcgcg aagcaggggc tgtccggtcc gagttggcta
     2701 cggccggacg ccccatcggt gcctacgacc tgctgattgc cgggcacgtc cgcagcctgg
     2761 acgcaaccct cgtgagccat aacgtccgag agttttctcg tgtgcccggg ctccggttgg
     2821 ccgattggac cggcggagcg gagccatctc acccgcaggc aggcactccc tagaggcgct
     2881 cgaagagacg cttgacttgt gcttccgggt cttctaggat tggctgcatc aggtcgtagc
     2941 aggacacgcc ggagggcgcg cggccccgcg aaagcttgcc ggcgtgggta ccaagcgttg
     3001 cacaaggcag gacgtggccc ggcatgagcc gagtcggtcc ggaagtgagt tggcacctca
     3061 cgaccggacc tggccacgaa cacaaggaaa ggaagtcctt atgtgtggtt gcacccatgg
     3121 tagcagctcg tccgaagtgt cccatcccgc caacgtcttc accgccgagt tcctggcccg
     3181 tctgggcgag agggacgagc cgcccacggc cggcgaggcc gcggtggcgg gtccgtggcg
     3241 gatcgagccg gtccctggga ggggcttcgg cctcttccgg gccggccagg gcctcgcccg
     3301 gggtcaccgg ccggtggcgg tctgcccctc gcgctgggcg gccctggtga ccgccgcggt
     3361 cctccccggc acggggcgcg atccgttgct ccggctgtcc aaggagcccg acacggacgg
     3421 catgtacccg gtgacgttgg acacgggcga ggccgtgatg cacctgaagt acttcgacga
     3481 gacgctggtg gatcacgcga acgccctcat ccacctgctg cgctccccgt cgtctctcgc
     3541 ggatgcgctg gaagccggcg gggctgaagc tctcggcctc tgcgggagcc tcctcgacga
     3601 gagattcccc ccgtccgaag acctgccgtc ccggtagcag gccacggcgc ccctgtccca
     3661 cgagtcaaag ccccggggag atgtctcgtt gccccggggc tctgtgttga agctccggca
     3721 attcggttcg aagtcccggg gggcgcactg aaactcccgg agctttggac caaggccccg
     3781 gggcatcagg tcagaacccc cggaccgcgt gtcaaagccc cgggatcttc actgaaagac
     3841 cgcgggcaac gcctcgaagc tccaagatga tcgatcaaag ctccggggtc ttcactcaag
     3901 ctcccgggag ctcgcatcag agccctgggg attcgcctcg tggtgccggg gcattcgctg
     3961 acaaccccgg gacattgaat cgaaggcccg gggctttgtg tcattgcttg ggcgcgccgt
     4021 agagcctggg aagcacctcc tctgttgaag cgctttcgtc tgctctcttg caaaggaggt
     4081 ctgtgatgaa gggccctcgg cgcaccctct ctctggtgac cgtgctgctt ctcgcggcgg
     4141 gaaccgccct ctcctacacc ctgacgagcc cgcagcgcac ctggaactgc ccgaccacct
     4201 acgtcgtcga ccagtccggc ctgcccagca tcgcggacgc ggacggcggg gcgacggcgg
     4261 tggtccaggc catcacctcc tcgaacgcct ggaacggcgc gggggcgggg cggatcatca
     4321 aggcgtccaa gggggatgtc tccggactga cctccgggga tggcatcccc atgatcagct
     4381 tctccgaccc gctgggcatc tgtaccggaa gctgcatcgc ggggagcctc aacggctact
     4441 ccttgcgcag tcccggctcc ggctcgtaca agctcaccga cgtcgatctc gtcttcaatc
     4501 cgtcctcatc cacttcctgg acctcacaag gagaggatcc aggaggcgcc ggctgctccg
     4561 gcgaggtcta catcgagggc ttcaccgtgc acgaggtcgg gcacggcctg ggcctcggcc
     4621 atacggccgt cgcgggggcg accatgtacc cgagcgtccc ttcttgcagc aacagccagg
     4681 cgaccaccga ggccgacgac gaggcgggcc tccgcgcgct ctacggcacc tcgccgtgtc
     4741 cggcctgcaa cagccacacg gagtacctgg cgtccctgga gcaggagatc cagccctgcg
     4801 ggagcgctta caagacgagc gtgacagggt tccatgaagg ctggctggag gggcccgcca
     4861 cgtccaattt cgacctctat ctggaccgct acaacgggct ggcctgggtg gtggtggcga
     4921 gctcgacggg agcggcctcc tcggaggcga tttcgtacaa cgggctcccg ggccgctaca
     4981 ggtatcgcgt ggtggcggcc agcggcacgg ggacctatca cttctgggaa cgacacccct
     5041 gaggggagcg gctatgagga gcacggcaga ccgctgccaa gatcgttcca ctctatttgg
     5101 aaagaggtga atcgatgaga ttccgcgctt tttgcaggca gttcatggtc gtctcgagtc
     5161 ttctgatggt cagctccgca ggcttcgcgg aagaagctgt cagagccacg cctgaatccc
     5221 cgcacgtgaa ggcggatgct caattcctgg cgatgctcgg ctcgacatcg gcacaaggag
     5281 ccgcgcagga gaagcaagga acatggggcg atcccgagtg ctgtgacacg aactggtact
     5341 gcgaggctgt gtattgtggt cacgtgggag ccacttgcga cgcggtcctg ggcccgaatt
     5401 gccccaggcg ctgctcgtgc ctctactagg ctgtcccggt agatcggagt ccgagcagcc
     5461 gtggcttgct ctgtactgtc tttactcagt gcaggtgggg gagaggccct ccgacggccg
     5521 gccggagggc acatccacta gcaccagcag gtacgagtct caggctgggg caggcagtag
     5581 cggcaggagt ctcctccgtt ggtaccgcac ccggcgcagg tggcttgcca tgtagtaccc
     5641 cccgctgctt ccagcaggtc gaatgccgaa agactcctca ccgtctcctt cgccagcgtc
     5701 agcttcttca cggtcttctt cattgtcact ccttggtagt atgcgccact tggcgcgagg
     5761 gacttatacc tcccccgggc tagcgcgtcg ttctgagcaa gacagcgaga gcagcgcggc
     5821 tctgtcgtct ttgagcgttt gctgagtgca aaggaagtgc ccggcggggg cacgctgact
     5881 gcgccgtggt cgggcttgac gcggcccgct ctcatgtaat agaatcggtt acatgatgag
     5941 gagcgaacgg ctctccgttg ccttgcacgt gctgctgcac atggccgagc ggcctgagga
     6001 gcccatgact tcccaggaga tggccgagtg cgtcggcacc aaccccgtgg tgatccgccg
     6061 cacgtttgcc ggcttgcggg aggccgggat cgtcagctcg gtcaaggggc acggaggcgg
     6121 atggaggctg gcgcggccgg cctcgtccgt gaacatcgcc gagatccagc gggcgctcgg
     6181 cgagcaggtc gtctccttca cttcgacggt ggagccgccg cgctgcgtgc tcctcaaggc
     6241 agtcatccat tcactcgacg aggcggtgaa ggaggccgag caggtcctcg agcgccgcct
     6301 ttcgacactt accctggcgg atctcgcgtc cagcgtcacc ggttcgcacg tccgtcctgg
     6361 aggtagaccc cgtgcaaaat gatgcgatcg tcgtcggcgg cagcttcgcc ggcctttccg
     6421 ccgcgatgca gcttgcccgt gcccgaaagc gggtgctggt gatcgacgcc ggccagcccc
     6481 gcaaccgttt cgccaagaca tcccatggct tcttcggcca ggacggcaag ccgcccgccg
     6541 agatcctgcg ggaggccgcc cggcaagtcg ccgcctatcc gaccgtcagg ttcatccagg
     6601 gcgaagcgac cggcgcgagc cagaccgacg gcggcttcac cgtgacgctg ggcgacgggc
     6661 gggaggagca ggccgcccgc ctggtgctcg cgaccggagt ccgcgacgag ctgccaccga
     6721 tcccggggat gcgggaacgg tggggccgaa ccgtcctgca ctgcccctac tgtcatggct
     6781 atgaggtcgc cggcggcgcg ctcggcgtcc tggccagcga tccggtgatg gcggtccaca
     6841 aagccatgct gatcccggac tggggccaga ccactctctt tacgcagggc ctcttcgagc
     6901 ccgacgcgga gcaggccgcg caactggccg cccgtggggt ccaggtggag cgtgcgccgg
     6961 tcgtggagct gctcggcgag gccccggagc tggaggccgt ccggctcgcc gacggccggg
     7021 tcatccccct gcaggcgatc ttcgcggcgc cggacgtgcg catggcgagc ccactcgcgg
     7081 aggcgctcgg ttgcgcgttc gagcagggcc cgcagggccc ctacatccgg gtggacgacc
     7141 ggaagcagac gaccgtcgcc ggcgtctacg ccgccggcga cgcgtcagcg gcgatgcaca
     7201 atgcgaccct cgcctcggcc agcggcgtgc tcgcgggcat cggggcccac cagtcgctcg
     7261 cgaaataaag tgacaggtcg aggggcggcc ggctggccgc cccgggtccc atccgattta
     7321 ccagccgcag ttcacaggcg gagcgccgcc gttcgcggtg ccgaacgaga agttgtcctg
     7381 gaactggcag ccgagctgat agaaattcgc tcccacggcg ttgcgaaagt tgaggcaggt
     7441 cgccgccgtc caggtggagg agacgacgat ggtgtcccgc caccaggggg tggtcgagta
     7501 ggcgctgcag agcttttggc cggtgaagct cacgccgttg ttgtctacga ccagcttgag
     7561 gacgccgtta tggtcgaaga agtgggcgat cgagcttgcg ctgctgccgg cgtggaacga
     7621 aaaggtgccg ccgaggtagc cggtctggat gcgcgcgccg ccggtcgaga gcagaccgta
     7681 ggtggcgtcg agcaggatgt tgccctggcg gatctccagc ttgtcgctcg ggctggaggt
     7741 cccgatcccg acattgccgc ccgagtaata gatgttcgaa ccgctgacct gccaggggcc
     7801 gccggccgac gtcccgccct gagcgaggag gtcccagttg gggaggccac tcgacggtgt
     7861 caccccgatg ttcggcgcgc ggccgatgta gctcgagccg ttatagctca cgagatcgtt
     7921 gacgttgtac gaaaccgagg gattccaggc gccgcgccag acgagctggc tgtcgtcggc
     7981 ggcgaagtag ccattgatgt cgaggatcat gtggacggcg gagaactcgg cctccacgtt
     8041 caacgcgccg ctgtttccca ggggcagaat cgccgcgttg gcgaggttct ggccgggctc
     8101 gtagttgatc gtcgaggtgt tgggccggac accccccgcc ggatagaaca ccaggtctcc
     8161 caggccgtcc gcctgggtga tggtgacgtt caccgacacc gcctgggccg tggccggcac
     8221 cgagcagtgg gcctgtccga tcatgacgag cgtccgcgga gtgccggggg cgagcttggg
     8281 gagcccatag cccgcgggca gggtggagcg ccgggtgtcc gcgatcctgc agggggtgat
     8341 tccggtgaag cggagaggcg gcgtgggcac cgccttctct cccgcggctt ccgcctccgg
     8401 gctccaggtc gcgctcgcga ccggccagtt ggcgatcttc tgtgcctcga gggttcctgc
     8461 caccggcaat ccaccgaggg ccaggaccgc caccatcgct ttcaacatcg tctgtctcct
     8521 ctctgatctg tgattcggca tgtactccag agacacgaga gaggcgagaa tgttgggaca
     8581 ggcgccaggg gctggccccg ggggagacgt ttgacacccc ggagcgaggt ccataggatg
     8641 ctgccgacgg cggcgggagg gcagcacggt accgggggga cgcgatgaca atcccgacag
     8701 aaaccagcct tttcgacggc ctccaggccg cacgggagct cgctgccgaa caggagcggc
     8761 tgcggatcac cctcgccagc atcggcgacg cggtgatcag caccgacgct gaaggccggg
     8821 tgacgtacct caacggggtg gccgaagcgc tgacggggtg gacccaggcc gaggccgccg
     8881 ggaggcccct cctcgaaatc ttccagatcc tcaatgaaga gacccgccag ccggcagaga
     8941 atccggccgc ccaggccctg cgcgaggggg tggtggtcgg gctggcgaac catacggtcc
     9001 tgatcgccag ggacggcacc gagaggccga tcgatgacag cgctgccccc atgctggaca
     9061 gctctggccg cccggtgggg gtggtgctgg tcttcc
//
LOCUS       DASPJI010000546         8645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_781836, whole genome shotgun sequence.
ACCESSION   DASPJI010000546 DASPJI010000000
VERSION     DASPJI010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8645)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781836"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VEL74_15050"
     CDS             <1..996
                     /locus_tag="VEL74_15050"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYG63893.1"
                     /translation="GLQVRGDDIGNGLFHTRERVRLATTRQDDILQLSGGPWVEARVR
                     WNPWLRTVAGLRADGYWADVESNLPENSGTEDDLLLSPKLSLLLGPWRRTEVYANFGY
                     GFHSNDARGATIRVDPRTGEAVGRVPPLVRAEGMDLGVRTEVVPGLNLALTGFVLDLA
                     SELVFVGDAGGTEASRPSRRAGVELQSFWRPRPWLSVDADLALSKGRFRDGDPAGEHI
                     PGSIERAVSAGVSVEDLGPVFGSLRLRYFGPRPLIEDDSIRSSSSTLVNARLGYAFRP
                     GLRLALEVFNLLDEDSNDIEYFYESRLPGEDEPVEDVHFHPHEPRALRLVAEWRY"
     gene            complement(993..2957)
                     /locus_tag="VEL74_15055"
     CDS             complement(993..2957)
                     /locus_tag="VEL74_15055"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYG63894.1"
                     /translation="MLRRPICPLFLCLVLTLSACRAREAPPPETVMEEPAAARAAGGP
                     AVTPPPPTVPEIAAAAKPGRKVILLGLDGADWELLDGYIAAGKMPNLARLVREGRSGT
                     LAAFQPSLCPLLWTTSVTGVSPLEHGILDYTRISPEGEKEEPVTSAERRVPAVWDMAT
                     AAGRSVAVLGMWGTYPAEPVRGLVVSDLWLAFDPKPNEMLPAGVVYPAEAEEWARTSL
                     RQAYNDRGGYEALAPWLPGLRQTDFENQAAQPDPYAHPVSALRQIRLETAAMGTPVTA
                     RLTADKPDLAIIYLQGPDTAGHVFAPFAPPRQEAVSAADFQRYGGVPERYFAEVDRLL
                     GMYREAAEATGVVLMLASDHGFLWKEGRPDRPGSASAQVGGQWHRPEGIYLLWGPGIE
                     KRSAREAGALDQVTPTLLALLGLPPARGLAGRPLPGVKASSASAADYRAHFRPGKPAP
                     GEQTRTVTSYNNEGLLLREKGEPEKAAAAFEKALAKEPDDAAALWNLSDLLFSTVGDD
                     RQKDRADELLMKALQAGLPAGTQRTIGRAVAYQKDGDLNRALRLLDAAVEVQPREPTL
                     WLFRGRYRIDSGRCPDALEDLRRARDLDDKNPLTYGALGLTRQCLGDIPGAVREYEKA
                     LDLDPGQSEIRQYLREITGGPAAPGNAGGF"
     gene            complement(2997..4847)
                     /locus_tag="VEL74_15060"
     CDS             complement(2997..4847)
                     /locus_tag="VEL74_15060"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HYG63895.1"
                     /translation="MRKAWSWSAASLALGLALSGPVPAATILERSVEIDIRPDGSVLE
                     RERLAVRLDTPADYSAWSSYPIFLDQNRTLEELSASATSPEGKVVKVGRKDQDTVDFT
                     SEGELHSSRQAKVVSFPVVPPGSVLRVDSTVREEPYFPAGAIFLGSSNPVEKLRVEVR
                     GAGADWRWRIDGSRDGLTVQESPGSVVITAANLPRREPPDLAPAEAGVHPVLRYGWRG
                     AATWQDVGRWYEGLLRDVPRAAEPVRRRARELAASAPSSRDRLAALLAFAAKQVRYVA
                     VEVGIGGYRPAPPEKVMERRWGDCKDKALLLIDLLREAGVEAYPALILLAEDERIDPE
                     FPSHDQFNHLIVAVAADGLAAPGDPVSEGLLFVDPTDETGSISWLHPGVQDQYALVVR
                     GDRSGLVRTPLRPALEETSLQVELKIEPDGSGRGGVRLDLEGDLGSSFQRLAAASRPE
                     EMEQGVRGVLGRLLPGAALSQVRWAQAGSDVPAFTISAQIQLPGLLPAGGLASSFSLP
                     SGANTPSTGLLQDRTEPLVLTPGTSLVSWRITLPDGACVPKPDEQTVENDLGAFRQTV
                     TLDGKVLKLERSLDLRQRWIDPSRFAAFKELALADQRSAKRRIRLECPAG"
     gene            complement(4816..6768)
                     /locus_tag="VEL74_15065"
     CDS             complement(4816..6768)
                     /locus_tag="VEL74_15065"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HYG63896.1"
                     /translation="MILPRKKLLLAPLLLALALGAVLPFRAAAAGFPEITAEERALTS
                     VPNQPNAPAVVLFKKGEFSMAGVLGNNDASILSVRIRRKILTDEGKRYGEVAVHHSKW
                     ARLQNLKARTVLPDNRVVPVAKEAIFRRKTSETGNTFVTAIAFPAVEVGAILDYSYEI
                     RIDSIFFLEPWYFQEDVPTLYSEMVCEMPEGIVTSSWRRDPMKVGIEQEVKQTVSGIL
                     VKVWGKNLPAVPSEPYSLPFADLASSFMLIPTAIQDGYGGVTKLFKDWASTCDIFGKA
                     YDKALRKSKAADARARELAKAGGGLREQAKAIHRFVRDEIETKDVPGVALAEGANVDA
                     VLAARQGDYAEKALLLQAMLGAVGIKAQPVWVADRSGGAVDMQLASPWWFDSLVVAAE
                     IDGQRVYLDPSDRTLAFGNLAPGLEGTPALLYDRKKPQPVILPTTRFEDHQRRAKVEL
                     GLDGEGRLAGRGTLELTGFHAWLRLDPQQEAAKVAESWKTWLGNAFEGYDVSDVQVKQ
                     EVDGQRLEVAWAMTQQEDEVLGDEVTLRPSLPFGPVQQPFTASRRLSPVLLAFADRDE
                     LELTLRWPEGWKPEALPKPLAHEGSVTEIAASVEVDEAARTLKYRRRMDIEQKFLQES
                     RQYEEIQNLYTLTERHDAQGLVLVRR"
     gene            complement(6895..>8645)
                     /locus_tag="VEL74_15070"
     CDS             complement(6895..>8645)
                     /locus_tag="VEL74_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYG63897.1"
                     /translation="RSRRGGPSAGRGLTAPSEGPAHLMRTPYLQQDLRPREPARPVEP
                     PPSQQSPEPSRSSVSPWSPLRHRVYRWLWTASLASNVGTWLQSVGASWLMTSLTTSTA
                     MVAMVQAATSLPMFLLSLPGGAIADVVDRRRLLLITQTWMALSAGGLALTTLAGGASP
                     WTLLLFTFLLGMGAAINGPAWQAIIPELVPREDLAGAVALGSIGFNIARAAGPALGGL
                     LVAAAGPGWTFLINAASFVGVIAVLYTWKRPEETSTLPAEQFLGAMRTGLRYVRHAPE
                     VIAVIVKGSAFVLCGSSIWALVPVVARNLGRGPSDYGLLLAAMGAGAVAGAMVLPRLR
                     SRHSADRVVAAATLVFAAATVALAWLRIFPLLFLAMLFAGGAWVSLLSSLNVSIQTSV
                     PSWVRARALSVYMLMFFGGLALGSALWGAMAERLGTPWALTVSAVGMIVGLVATARVQ
                     LRSGEGLNLAPSRQWPAPMVAQDLEHDRGPVLVTVEYRIDPARAAEFAAAMRHIRRIR
                     LRDGALQWGLFADAAVPGLYTQVFLVQSWAEHLRQHERATVADRDIEREAWSFHLGPE
                     PPVVRHLIGERVREPR"
BASE COUNT         1237 a         3059 c         3132 g         1217 t
ORIGIN      
        1 ggcctccagg tgcggggaga cgacatcggc aacggtctgt tccacacccg ggagcgggtc
       61 cgcctcgcca ccacccggca ggacgacatc ctccagctct ccggcggccc ctgggtggag
      121 gcccgggtgc gctggaaccc ctggctccgc accgtcgccg gcctgcgcgc cgacggctac
      181 tgggccgacg tggagagcaa cctgccggag aactccggca ccgaggacga cctgctgctg
      241 tcgccgaagc tgtcgctcct cctcggcccc tggcgccgga ccgaggtcta cgccaacttc
      301 ggctacggct tccacagcaa cgacgcccgg ggcgccacga tccgggtcga tccgcggacg
      361 ggggaggcgg tgggccgggt gccgccgctg gtgcgggccg aggggatgga cctgggggtg
      421 cgcaccgagg tggtgccggg gctcaacctg gcgctgaccg gcttcgtcct cgacctggcc
      481 tcggagctgg tcttcgtcgg cgacgctggc ggcaccgagg ccagccgccc gagccgccgc
      541 gccggcgtcg agctccagag cttctggcgc ccccgcccct ggctgtcggt ggacgccgac
      601 ctggctctct ccaagggccg cttccgggac ggcgacccgg cgggggagca catccccggc
      661 tccatcgaga gagcggtgtc ggccggtgtg tcggtggagg atctgggccc ggtgttcggc
      721 agcctgcgcc tccgctactt cggcccccgc ccgctgatcg aggacgacag catccgctcc
      781 tcgtcctcga cgctggtcaa cgcccggctg ggctatgcct tccgtcccgg cctgcgcctg
      841 gccctggagg tcttcaacct cctggacgag gactcgaacg acatcgagta cttctacgag
      901 tcccgtctac ccggagagga cgagccggtg gaggatgtcc atttccatcc ccacgagccc
      961 cgggcgttgc ggctggtggc ggagtggagg tattagaacc cgccggcgtt tccaggagcg
     1021 gcgggcccgc ccgtgatctc ccgcaggtat tgccggatct cggactgacc gggatcgaga
     1081 tccagcgcct tctcgtactc ccgcacggcg cccgggatgt ctcccaggca ctggcgggtg
     1141 agccccaggg cgccgtaggt gagggggttc ttgtcgtcga ggtcccgggc gcgccgcagg
     1201 tcttccagcg cgtcggggca gcggccggag tcgatccggt accggccacg gaagagccag
     1261 agggtgggct cacgcggctg gacctcgacc gccgcatcca gcagacgcag cgcccgattc
     1321 aggtcgccat ccttctggta ggccacggcc cgtccgatgg tgcgctgggt gccggcgggc
     1381 agtccggcct gcaacgcctt catcagcagc tcgtcggcgc ggtccttctg gcggtcgtcg
     1441 ccgacggtgg agaagaggag gtcgctcagg ttccacaggg ccgcggcatc gtccggctct
     1501 ttggcgagcg ccttttcgaa cgccgccgcg gccttctccg gctccccctt ctcgcggagc
     1561 agcaatcctt cgttgttgta ggaggtcacg gtgcgggtct gctcccccgg cgccggcttg
     1621 cccggccgga agtgggcccg gtagtcggcg gcggaagcgg atgaggcctt caccccgggc
     1681 agcggccggc cggcaaggcc gcgggcgggc ggcaggccga ggagcgcgag gagcgtcggc
     1741 gtcacctgat cgagggcgcc ggcctctctc gcggagcgtt tctcgatgcc cggaccccag
     1801 aggagataga tcccctccgg ccggtgccac tggccgccga cctgggcact ggcgctgccg
     1861 ggccggtcgg ggcgcccctc cttccagagg aagccgtggt ccgaagccag catcagcacc
     1921 acgccggtgg cctcggcagc ctcccggtac atcccgagca ggcggtcgac ctcggcgaag
     1981 tagcgctccg gcaccccgcc atagcgctgg aaatcggcgg cggagaccgc ctcctgccgt
     2041 ggcggggcga agggggcgaa gacatggccg gcggtgtccg gtccctggag atagatgatg
     2101 gcgaggtccg gcttgtccgc cgtcagccgg gcggtcaccg gagtccccat ggccgccgtc
     2161 tcgaggcgga tctgccgcag ggcgctcacc ggatgggcgt aggggtccgg ctgcgcggcc
     2221 tgattctcga agtcggtctg ccggaggccg gggagccacg gcgccagggc ctcgtagccg
     2281 ccgcggtcgt tgtacgcttg gcgcaggctg gtgcgcgccc actcctcggc ctcggcggga
     2341 tagaccaccc ccgccggcag catctcgttg ggcttgggat cgaaggcgag ccagaggtcg
     2401 gagaccacga gaccccgcac cggctcggcg gggtaggtgc cccacatccc gagcaccgcg
     2461 acgctccggc ccgcggcggt ggccatgtcc cacaccgccg gcacccggcg ctcggcgctg
     2521 gtgaccggct cctccttctc cccttcggga gagatccggg tgtagtccag gatgccgtgc
     2581 tccagcgggc tgacgccggt gaccgaggtc gtccagagga gggggcagag cgagggctgg
     2641 aaggcggcca gggtcccgga ccgcccctcg cggacgagcc gcgccaggtt gggcatcttg
     2701 ccagccgcga tgtatccgtc cagcagctcc cagtcggcgc cgtcgaggcc gaggaggatc
     2761 accttgcgtc cgggcttggc ggcggccgcg atctccggca ccgtcggcgg cgggggagtg
     2821 accgcgggcc cccctgcggc ccgagcggcg gccggctctt ccatcaccgt ctccggcggc
     2881 ggcgcctccc gcgcccggca ggcggacagg gtcaggacca gacagaggaa cagcggacag
     2941 atgggacgtc gcagcatggg ggcggcctcg gttggccgcc cttataccat gccgctctat
     3001 cccgccgggc actccagccg gatgcgccgc ttggccgacc gctgatccgc cagggccagc
     3061 tccttgaagg ccgcgaacct ggagggatcg atccagcgct ggcggaggtc cagggagcgc
     3121 tcgagcttca agaccttgcc gtcgagggtg accgtctgcc ggaaggcgcc caggtcgttc
     3181 tccaccgtct gctcgtccgg cttggggacg caggcgccgt cgggcagggt gatgcgccag
     3241 gagaccaggg acgtgccggg cgtcagcacc agcggctcgg tgcggtcctg gaggaggccg
     3301 gtggacgggg tgttggcgcc cgacggcaac gagaacgagc tggcgaggcc gccagcgggg
     3361 agcaatcccg ggagctggat ctgggccgag atcgtgaacg ccggcacgtc cgagccggcc
     3421 tgggcccagc ggacctgcga cagggcggcg ccggggagca gccggccgag cacgccgcgc
     3481 acgccctgct ccatctcctc ggggcgtgac gcggcggcca ggcgctggaa gctcgatccc
     3541 aggtcccctt cgaggtcgag gcgcacgccg ccccggccgc tgccgtccgg ctcgatcttc
     3601 agctccacct ggaggctcgt ctcctcgagc gccggccgca acggcgtccg caccagtccg
     3661 gaccggtccc cccgcaccac cagggcgtac tggtcctgca cgccgggatg gagccaggag
     3721 atggagccgg tctcgtccgt gggatcgacg aataggagcc cctcggacac cgggtccccg
     3781 ggcgcggcga ggccgtcggc cgccaccgcc acgatgaggt gattgaactg gtcgtgcgag
     3841 gggaactcgg ggtcgatcct ctcgtcctcg gccagcagga tgagggcggg gtaggcctcg
     3901 acgccggcct cgcgcagcag gtcgatcagg agcagcgcct tgtccttgca gtcgccccag
     3961 cggcgctcca tcaccttctc cggcggtgct ggccgatagc cgccgatccc cacctccacc
     4021 gccacgtagc gcacctgctt ggcggcgaag gcgagaagag cggcgagccg gtcgcgggac
     4081 gacggagcgc tggcggccag ctcccgcgcc cggcgccgca ccggctccgc ggcccgcggg
     4141 acgtcgcgca gcagcccctc gtaccagcgc cccacgtcct gccaggtggc ggcgccgcgc
     4201 caaccgtagc gcaggaccgg gtggacgccc gcttccgccg gcgccaggtc cggcggctcg
     4261 cggcggggga ggttggcggc ggtgatcacc acgctgccgg ggctctcctg gactgtcagg
     4321 ccgtcgcggg agccgtcgat ccgccagcgc cagtccgccc cggctccacg tacctccacc
     4381 cgcagcttct cgaccggatt ggacgacccc aggaagatgg cgccggccgg gaagtagggc
     4441 tcctcccgca ccgtcgaatc gacgcgcagc accgaccccg gcggcaccac gggaaagctc
     4501 accaccttgg cctggcgcga cgagtggagc tccccctcgg aagtgaagtc caccgtgtcc
     4561 tggtccttgc ggcccacctt gaccaccttg ccctcgggag aggtggcgga ggcgctcagc
     4621 tcttccagcg tccggttctg gtccaggaag atcggatagg aggaccacgc ggagtagtcc
     4681 gccggcgtgt cgagccggac cgccaggcgc tcgcgctcga gcaccgagcc gtccggacgg
     4741 atgtcgatct ccacgctccg ctccaggatc gtcgccgccg gcaccggccc cgagagggcc
     4801 agcccgaggg ccagactagc ggcggaccag gaccaggcct tgcgcatcgt gcctctccgt
     4861 cagggtgtag aggttctgga tttcctcata ctgccgggac tcctgcagga atttctgctc
     4921 gatgtccatg cggcgacggt acttgagggt gcgcgcggcc tcgtcgacct cgacgctggc
     4981 ggcgatctcg gtgacgctgc cctcgtgggc cagcggcttg ggaagggcct cgggcttcca
     5041 tccctccggc cagcgcagcg tcagctcgag ctcgtcgcgg tcggcgaagg cgagcaggac
     5101 cggcgagagc cggcgtgacg ccgtgaacgg ctgctgcacg ggcccgaagg ggaggctcgg
     5161 ccggagcgtc acctcatctc ccagcacctc gtcctcctgc tgcgtcatcg cccaggcgac
     5221 ctcgagccgc tggccgtcga cctcctgctt cacctggacg tcgctgacgt cgtagccctc
     5281 gaaggcgttg ccgagccagg tcttccagga ctcggcgacc ttggcggcct cctgctgcgg
     5341 gtcgaggcgg agccaggcgt ggaagccggt cagctccagg gtgccgcgcc cggccaggcg
     5401 cccctcgccg tcgaggccca gctccacctt ggcgcggcgc tggtgatcct cgaagcgggt
     5461 ggtggggagg atgaccggct gcggcttctt gcggtcgtag aggagggccg gcgtcccttc
     5521 gagcccggga gccaggttgc cgaacgcgag cgtgcggtcg gagggatcga ggtagacgcg
     5581 ctggccgtcg atctccgccg ccaccaccag gctgtcgaac caccaggggc tggcgagctg
     5641 catgtccacg gcgccgccgg agcggtcggc tacccagacg ggctgggcct tgatcccgac
     5701 ggcccccagc atcgcctgga ggagcagggc cttctcggcg tagtctccct gcctggcggc
     5761 gagcacggcg tcgacgttgg cgccctccgc gagcgcgacg cccggcacgt ccttggtctc
     5821 gatctcgtcg cgcacgaagc gatggatggc cttcgcctgc tcgcggagtc cgccgccggc
     5881 cttggccagc tcccgcgccc tggcgtcggc ggccttggac ttgcgcaatg ccttgtcgta
     5941 ggccttgccg aaaatatcgc aggtgctcgc ccagtccttg aacagcttgg tgacaccgcc
     6001 gtagccgtcc tggatggcgg tgggaatcag catgaagctg ctggcgaggt cggcgaacgg
     6061 caggctgtag ggctcggagg gcaccgccgg caggttcttg ccccagacct tcaccaaaat
     6121 accgctgacc gtctgcttca cctcctgctc gatgccgacc ttcatcggat cgcgccgcca
     6181 actgctggtg acgatcccct cgggcatctc gcagaccatc tcggagtaga gggtgggcac
     6241 gtcctcctgg aagtaccagg gctcgaggaa gaagatgctg tcgatccgga tctcgtagga
     6301 gtagtcgagg atggcgccca cctcgaccgc cgggaaggcg atggcggtga cgaacgtgtt
     6361 gccggtctcc gaggtcttgc gacggaagat ggcctccttg gccaccggca ccacccggtt
     6421 gtcgggcagc acggtccgcg ccttgaggtt ctgcaggcgc gcccacttgc tgtggtggac
     6481 cgccacctcg ccatagcgct tgccctcgtc ggtcaggatc ttgcggcgga tgcggacgct
     6541 gaggatggag gcgtcgttgt tgccgagcac gccggccatg gagaactcgc ccttcttgaa
     6601 cagcaccacg gccggagcgt tgggctggtt gggcacgctg gtcagggcgc gctcctcggc
     6661 ggtgatctcc gggaagccgg cggccgcggc ccggaagggg aggacggctc cgagcgccag
     6721 ggcgagcagc aggggagcga gaagcagctt cttgcgggga aggatcatga gatggctctc
     6781 cttgtcattc tctcgcaagt gagccggcgc gggaatccat ttcgcgaccg gagcccccgg
     6841 ggaatgtcgg cgcgccgaca tgaacccctt ttgcgtgaga cggaggggtg gagcctacct
     6901 cggctccctc acccgttccc cgatgaggtg ccgcaccacc ggcggctccg ggccgaggtg
     6961 gaaggaccat gcctccctct cgatatcccg gtccgccacg gtagcccgct cgtgctggcg
     7021 gaggtgctcg gcccaggact ggaccaggaa gacctgggta tagaggcccg gcacggcggc
     7081 gtcggcgaag aggccccatt gcagggcgcc gtcccgcagg cggatgcggc ggatgtgccg
     7141 catggccgcc gcgaactccg ccgcccgggc cggatcgatc cggtactcga cagtcaccag
     7201 caccgggccg cggtcgtgtt cgaggtcctg cgcgaccatg ggcgccggcc actgccgcga
     7261 gggggccagg ttgagcccct cgccggagcg gagctggacc cgcgcggtgg cgaccagccc
     7321 tacgatcatc cccactgccg agacggtgag cgcccagggg gtaccgagcc gctcggccat
     7381 cgccccccag agggcgctgc cgagggccag gccgccgaag aacatcagca tgtagaccga
     7441 cagggcccgc gcccgcaccc aggaggggac gctggtctgg atcgacacgt tgaggctgga
     7501 cagcagcgag acccaggcgc cgccggcgaa cagcatcgcc aggaagagga gagggaagat
     7561 ccgcagccag gcgagcgcca ccgtcgccgc ggcgaagacc agcgtggccg ccgccaccac
     7621 ccggtccgcc gaatgacggc tccgcaggcg gggaaggacc atggccccgg ccaccgctcc
     7681 ggctcccatc gccgccagca gcaggccata gtccgacggg ccccgcccca ggttgcgggc
     7741 gaccaccggc accagcgccc agatcgagct gccgcagagc acgaaggcgc tccccttgac
     7801 gatgaccgcg atgacctcgg gcgcgtggcg cacgtagcgc agcccggtcc gcatcgcccc
     7861 caggaactgc tcggcgggga gcgtcgacgt ctcctccggc cgcttccatg tatagaggac
     7921 ggcgatcacc cccacgaagg aggcggcgtt gatcaggaac gtccaccccg gaccggccgc
     7981 ggctaccagc aatcctccca gcgccggacc ggcggcgcgg gcgatgttga agccgatgct
     8041 tcccagagcc acggcgccgg cgaggtcctc ccgcggcacc agctccggga tgatcgcctg
     8101 ccacgcaggg ccgttgatcg ccgcgcccat ccccagcagg aaggtgaaga ggagcagggt
     8161 ccagggggag gcgccgccgg cgagggtggt caaggcgaga ccgccggccg atagcgccat
     8221 ccaggtctgg gtgatgagga ggagccggcg gcggtcgacc acatcggcga tggcgccgcc
     8281 ggggagcgac aggaggaaca tgggcaggct cgtcgccgcc tggaccatgg ccaccatcgc
     8341 cgtcgaggtg gtgagcgagg tcatcagcca cgacgccccc acgctctgca gccacgttcc
     8401 gacgttcgac gccaggctcg ccgtccacag ccagcgatag acccggtgcc gcagcgggct
     8461 ccagggggag accgaagagc gagacggctc cggtgactgc tgggaaggcg gcggctccac
     8521 cggacgcgcc ggttcccgtg gcctgaggtc ttgttgtaaa tacggggtgc gcatcaggtg
     8581 ggcaggtcct tcagagggag ccgtcaggcc gcggccggca gacggacctc cacggcggct
     8641 cctcc
//
LOCUS       DASPJI010000547         3166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_497120, whole genome shotgun sequence.
ACCESSION   DASPJI010000547 DASPJI010000000
VERSION     DASPJI010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497120"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1950)
                     /gene="pbpC"
                     /locus_tag="VEL74_15075"
     CDS             complement(<1..1950)
                     /gene="pbpC"
                     /locus_tag="VEL74_15075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HYG63898.1"
                     /translation="MRTLWGTPWRASLLLVFALASPFLLFLLLDLLFPFPWEALEKPP
                     AVVVLDRQGEPLRFFLPSDDRWRFPVRLSELPSELPRALVASEDQRFWRHPGVDPLAV
                     LRAAWANTRAGEVVSGASTIPMQIARMAEPGPRTLPSKVRESFRALQLERRYSKDELL
                     EIYLNLAPYGGNVEGVGAAAWFYFGKRPEQLSLSEIALLTALPRSPVGYDPVRRPQAA
                     EAARDRVLAQLTARGAFPRAEIAAASRQPLPRERGRAPFAAPHLTEWAVQRFPGQERL
                     RITLDRGVQRIVEEQVRRRSGELRDQGIGNAAVVVIDNETRAVRALAGSADFFERDAS
                     GQVNGAVARRSPGSTLKPFLYAMALDEGRVLPDSYLLDIPTDFGGYVAENYDERYRGR
                     VTVRDALVQSLNACAVRLLAEVGLGDYLKLLRRGGLATLDREPGQYGLPLVLGAGEVT
                     LVDLANLYATLAEGGVHRPVRLLEPEGDAAGAPPEETDRLFSRDAARAITGILTDVRR
                     PDLPESWDLTRGVPAVAWKTGTSYGHRDAWAVGFSARTTIGVWVGNFDGRPRKGISGS
                     QHAGPLLFDLFRALEERDGGPARPPVDAVPDETLEVCADSRELPGPFCPRRLHVPYLP
                     ERSRLTACTYHRRVLVDAETGDLLAG"
     gene            complement(2012..>3166)
                     /locus_tag="VEL74_15080"
     CDS             complement(2012..>3166)
                     /locus_tag="VEL74_15080"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HYG63899.1"
                     /translation="HFLAEARRAGHPVDEGMLNRALAWVAGQVKAKSAYGSEELQRAV
                     YGLYVLARAGKADQGTMDFIREKHLGSLRPESRAMLAAAYATTGNPRALQTLLSNLGE
                     VEQVERQTNGNFNSTIRNRAMLLLALLDAAPNSPRIPALADRLARDAREVRQWNTQEE
                     GFTLIALGQLVQRQAKMPPYSGSVFVGGKKVGTFTNKTVTFRALRGDGPIRLQMDAGY
                     KGGAAFYHVLTRGVPTDDAYRPASAGLEVERALLNREGQPLDLGNVRQGDLVVMRTRV
                     RSVSGPVSNVAVVNLLPSGLEVENPRLSTTEQTPWVADAGALDYLDLRDDRILLFVDL
                     PPNSWQTYYTLARAVAPGSFRLPPVQAEAMYNPALRAAGERGTIEVKLRE"
BASE COUNT          454 a         1115 c         1186 g          411 t
ORIGIN      
        1 gccggccagg aggtcgccgg tctcggcatc caccagcacc cggcggtggt aggtgcaggc
       61 ggtcaggcgg gagcgctccg gcaggtaggg gacgtgcagc cgccgaggac agaagggtcc
      121 cggcagctcg cggctgtcgg cgcagacctc gagggtctcg tcggggaccg catccaccgg
      181 tgggcgggct ggccccccat cccgctcctc cagggcgcgg aacaggtcga aaagcagcgg
      241 cccggcgtgc tggctgcccg agatcccctt gcgcggccgg ccgtcgaagt tgcccaccca
      301 gacgccgatg gtggtgcggg cggagaagcc gaccgcccag gcgtcgcggt ggccgtagga
      361 ggtgccggtc ttccaggcga cggcgggcac gccgcgggtg aggtcccacg actccggcag
      421 gtcggggcgg cgcacgtcgg tgaggatacc ggtgatcgcc cgcgccgcgt cgcgggagaa
      481 caggcggtcg gtctcctccg gcggcgcgcc ggccgcgtct ccctccggct ccagcaggcg
      541 gaccggacgg tggacgcccc cttcggccag ggtggcgtag aggttggcga ggtcgaccag
      601 ggtcacctcg ccggccccga ggaccagggg gaggccgtac tgcccgggct cgcggtccag
      661 ggtggcgagg cccccccggc gcagcagctt cagatagtcg ccgagcccca cctcggcgag
      721 caggcggacg gcgcaggcgt tgagcgactg caccagggcg tcgcgcacgg tgacccggcc
      781 gcggtagcgc tcgtcgtagt tctccgccac gtagccgccg aagtcggtgg gtatatccaa
      841 tagataagag tcggggagga cccgcccctc gtccagggcc atggcataga gaaagggctt
      901 gagcgtcgag ccgggggagc ggcgggccac ggcgccgttg acctggccgg aggcgtcccg
      961 ctcgaagaag tcggccgacc cggcgagcgc ccgcaccgcc cgggtctcgt tgtcgatgac
     1021 gaccacggcg gcgttgccga tcccctggtc gcgcagctct ccgctccggc gccgcacctg
     1081 ctcctcgacg atcctctgca ccccccggtc gagtgtgata cgcagccgct cctgcccggg
     1141 gaagcgctgg acggcccact cggtgaggtg aggggcggcg aagggggccc ggccgcgctc
     1201 ccggggcagg ggctggcgcg aggccgcggc gatctccgcg cgggggaagg cgccgcgcgc
     1261 ggtgagctgg gcgagcaccc ggtcccgcgc cgcctcggcc gcctgcggcc gccgcaccgg
     1321 gtcgtagccc acgggcgagc gcggcagcgc ggtgaggagc gcgatctccg acagcgagag
     1381 ctgctccggc cgcttgccga aatagaacca ggccgccgcc ccaaccccct cgacgttgcc
     1441 gccgtagggg gcgaggttca ggtagatctc cagcagctcg tccttggagt agcggcgctc
     1501 gagctggagg gcgcggaagc tctcccgcac cttggacggc agcgtccgcg gccccggctc
     1561 ggccatgcgg gcgatctgca tggggatcgt cgaggcgccg gagaccacct ctccggcccg
     1621 agtgttggcc cacgccgccc gcagcaccgc caggggatcg acgccgggat ggcgccagaa
     1681 gcgctgatcc tcggacgcca ccagcgcccg ggggagctcg gagggaagct cggacagccg
     1741 caccgggaac cgccagcggt cgtcggacgg caggaagaac cgcagcggct ccccctgccg
     1801 gtccagcacc accacggcgg gcggcttctc cagggcctcc caggggaagg ggaagaggag
     1861 gtctaggagg aggaagagga ggaaggggga ggccagggcg aataccagga ggagggatgc
     1921 tctccagggg gttccccaga gagtcctcac ccatacgggc agacctcccc ccatacgaac
     1981 agcttccttc tgagacacct tcaacctgcc cctactccct gagcttcacc tcgatcgtcc
     2041 cccgctcgcc ggccgcgcgg agggcggggt tgtacatggc ctcggcctgc accggcggca
     2101 gccggaagct ccccggcgcc accgcgcggg ccagcgtgta gtaggtctgc cacgagttgg
     2161 ggggcagatc gacgaagagc aggatgcgat cgtcgcgcag gtcgaggtag tcgagcgcgc
     2221 cggcgtcggc cacccagggc gtctgctcgg tggtcgagag gcgcgggttc tccacctcga
     2281 ggcccgaggg gaggaggttg accacggcga cgttcgagac cgggccggag acgctgcgca
     2341 cccgggtgcg catcaccacc aggtccccct gccggacgtt gccgaggtcg agcggctgtc
     2401 cctcgcggtt cagaagcgcc cgctccacct ccaggcccgc cgacgccggc ctgtaggcgt
     2461 cgtcggtggg cacgccccgg gtgagcacgt ggtagaaggc cgcccctccc ttgtagcccg
     2521 cgtccatctg gagacgaatg ggcccgtcgc cgcgcagtgc ccggaaggtg acggtcttgt
     2581 tggtgaaagt gcccaccttc ttgccgccga cgaagactga cccggagtag gggggcatct
     2641 tcgcctggcg ctgcacgagc tggcccaggg cgatgagggt gaagccctcc tcctgggtgt
     2701 tccactgccg gacctcgcgg gcgtcgcggg ccaggcggtc ggcgagcgcc gggatgcgcg
     2761 gactgttggg cgccgcgtcc agcagcgcga gcaggagcat cgcccggttg cggatcgtcg
     2821 agttgaagtt gccattcgtc tggcgctcca cctgctccac ctcgcccagg ttcgacagca
     2881 acgtctgcaa cgcccgggga ttgccggtcg tggcataggc cgccgccagc atcgcccgcg
     2941 actcggggcg caggctcccc aggtgcttct cgcggatgaa gtccatggtc ccctgatccg
     3001 ccttgccggc gcgggcgagg acgtagaggc cgtagacggc gcgctgcagc tcctcgctgc
     3061 cgtacgccga cttggccttg acctgcccgg ccacccaggc cagggcgcgg ttgagcatcc
     3121 cctcgtccac cggatggccg gcccgccgcg cctcagccag gaagtg
//
LOCUS       DASPJI010000548         2643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_782248, whole genome shotgun sequence.
ACCESSION   DASPJI010000548 DASPJI010000000
VERSION     DASPJI010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782248"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VEL74_15085"
     CDS             complement(<1..227)
                     /locus_tag="VEL74_15085"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYG63900.1"
                     /translation="MTDPDSEDFTEAVAVIGMAGRFPGARNLDEFWRNLRDGVETVRR
                     FSDEEMLAEGMDPATLAHPSFVPVGAILDDV"
     gene            complement(227..>2643)
                     /locus_tag="VEL74_15090"
     CDS             complement(227..>2643)
                     /locus_tag="VEL74_15090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63901.1"
                     /translation="ELEGLIGFFINTLVLRGDLSGDPAFSGLVRRLREVTLGAFDHGQ
                     VPFERLLESLRVERTLSHTPLFQVMLLLQSFPAASVEPMEVELSPVTLDDERSNFDLS
                     LWLTEADEGFVGSLEHNLDLFDPPTIHRLGRQLLTLLRSALASPGKPLSELPLLGDAE
                     RHQLLLEWNDTAEMAAAQPAVHRQFQTAARRSPAAPALLWPAGEMTYGEVEEKARRLA
                     RRLRRAGAGPGVVVGIFLDRSPELIVALLAVLETGAAYLPLDPDYPADRLAFLLEDSG
                     AHVLLTREALLPSLPEHRAEVLRVDEEEDGPAFEQEVDPDQPVYILYTSGSTGQPKGV
                     VVRHGSLARYVETARRAYGLEPEDRVLQFASIGFDTSAEEIYPCLAAGATLVLRSGPR
                     AEEIPEFLAGVGRLGITVLDLPTAYWHEIVAALERDPSLAVPPGVRLVILGGERALPG
                     RVRAWRERVPAARLVNTYGPTEGTIVATHSDLSTAPWERRMEAEVPIGRPVDGVRTYL
                     LDRALRPVPLGVWGELCLGGGGLAQGYLGKPGLTAERFVPAPFEGPGLRLYRTGDLAR
                     FRPDGALEFGGRVDFQVKVRGVRVEPGEVEAALARHPALREAAVGAVADPAGGVRLVG
                     WIAAQPGQSPTVSELRGFLLERLPEAMVPSAFVILPALPLLPNGKVDRRALPVPGGER
                     PDLGTAYTAPETELERAIARVWQELLRVDRVGLGDNFFDLGGHSLLLVQAQGRLSEAL
                     GREVAVVDLFRFPTVQSLARHLGGTDAGAAAARKAQDLGEKQRTAMSKQRQAMAAAQS
                     RLKKKP"
BASE COUNT          413 a          931 c          959 g          340 t
ORIGIN      
        1 tcgacgtcat cgaggatcgc gcccacgggc acgaaactgg gatgagcgag cgtcgccggg
       61 tccatgcctt cggcgagcat ctcctcgtcg ctgaaccggc gcaccgtctc gacgccgtcg
      121 cgcaggttgc gccagaactc gtcaaggttc cgcgctccgg ggaaccggcc ggccataccg
      181 atgacggcga ccgcctcggt gaagtcttcg ctatcgggat ctgtcatcac ggtttcttct
      241 tgagtctcga ctgtgcggcg gccatggcct gccgctgctt gctcatcgcg gtccgctgct
      301 tctcgccgag gtcctgggcc ttcctggccg cggcggctcc ggcgtccgtt ccgcccaggt
      361 gacgggcgag cgactggacg gtggggaaac ggaagaggtc caccaccgcg acctcgcggc
      421 cgagcgcctc gctcagccgg ccctgcgcct gcaccagcag caacgagtgg ccgccgaggt
      481 cgaaaaagtt gtcgccgagc ccgacgcgat cgacgcgcag cagctcttgc cagacgcggg
      541 cgatggcacg ctcgagctcg gtctccggcg ccgtgtaggc cgtgcccaag tcggggcgct
      601 cgccgccggg gacgggcagg gcgcggcggt cgaccttgcc gttcggcagc agcggcaggg
      661 ccggcaggat cacgaacgcc gacggcacca tggcctcggg caggcgctcg agcaggaagc
      721 cgcgcagctc ggagacggtc ggcgactgcc cgggctgggc cgcgatccag cccaccagcc
      781 ggacgccccc tgccggatcg gccacagcgc cgacagcggc ttcgcggagc gcggggtggc
      841 gcgccagagc agcctccacc tccccaggct cgacgcgtac gccgcgcacc ttgacctgga
      901 agtcgacgcg cccgccgaac tccagggcgc cgtcggggcg gaagcgcgcc agatcgccag
      961 tccggtagag ccggagcccg ggcccctcga acggggcggg cacgaagcgc tcggcggtca
     1021 agccgggctt ccccagataa ccctgggcga ggccgccgcc gccgagacaa agctcacccc
     1081 agacgcccag cggcaccggc cgcagggcgc ggtcgagcag gtaggtccgg accccgtcca
     1141 ccggccggcc gatgggcact tcggcctcca tgcgccgctc ccacggcgcg gtgctcaggt
     1201 cggagtgggt ggcgacgatc gttccctcgg tcggaccgta ggtgttgacc agacgcgcgg
     1261 ccggcacccg ctcgcgccag gcgcggaccc gccccggcag ggcgcgctcc ccacccagga
     1321 tcaccagccg cacgcccggc ggaacggcga gggacgggtc cctctccagc gccgcgacga
     1381 tctcgtgcca gtaggcggtg ggcaggtcga gcaccgtgat gccgagccgc ccgacgccgg
     1441 cgaggaactc ggggatctct tcggcccgcg gtccggagcg cagcaccagc gtcgcacccg
     1501 ccgccaggca ggggtagatc tcctcggcac tggtgtcgaa gccgatggac gcgaactgca
     1561 ggacgcggtc ctccggctcc aagccgtagg cccggcgcgc agtctccacg taccgagcca
     1621 gcgatccatg ccgtaccacg acccctttgg gctgtccggt ggagccggat gtatagagaa
     1681 tgtagaccgg ctggtcggga tcgacctcct gctcgaaggc gggcccgtcc tcttcctcgt
     1741 cgaccctgag gacctcggcc cggtgctccg ggagcgacgg cagcagagcc tcgcgggtga
     1801 ggagcacatg ggcgccggag tcctccagca ggaaggcgag acggtccgcc ggatagtcgg
     1861 ggtcgagcgg caggtaagcg gcgccggtct cgagcacggc gagcagggcg acgatcagct
     1921 caggggagcg gtcgaggaag ataccgacga ccacaccagg accggcaccg gcccgccgca
     1981 gccggcgggc gagccgccgg gccttctctt cgacctcgcc gtaggtcatc tcccccgccg
     2041 gccagaggag ggccggtgcc gcgggcgagc gccgggccgc ggtctggaac tggcggtgga
     2101 ccgctggctg cgccgcagcc atctcggcgg tgtcgttcca ctcgagcagg agctgatggc
     2161 gctcggcatc accgagcagc ggcagctcgg agagcggctt cccagggctg gcgagcgcgc
     2221 tccgcagcag ggtcaggagt tggcggccca ggcggtggat ggtcggtgga tcgaacaggt
     2281 cgaggttgtg ctccaggctg ccgacgaatc cttcgtccgc ttcggtcaac cacaggctga
     2341 ggtcgaagtt ggaccgctcg tcgtcaaggg tgaccggcga cagctcgacc tccatgggct
     2401 cgacggatgc cgcggggaag ctctgtagca gcagcatcac ctggaacagc ggagtgtggg
     2461 agagggtacg ctcgacgcgc aacgactcga gcagccgttc gaagggcacc tggccatggt
     2521 cgaaggcacc gagcgttacc tcccgcagac gccgcacgag cccggagaag gcggggtccc
     2581 ccgagaggtc gccgcgcaag acaagggtgt tgatgaagaa gccgatcagc ccctccagct
     2641 cgg
//
LOCUS       DASPJI010000549         7572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_435752, whole genome shotgun sequence.
ACCESSION   DASPJI010000549 DASPJI010000000
VERSION     DASPJI010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7572)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435752"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /locus_tag="VEL74_15095"
     CDS             complement(<1..1665)
                     /locus_tag="VEL74_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020601066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HYG63902.1"
                     /translation="MHRPVSRPLWTAAALFTLACGAAAQPPAVNPQITDAAVTDQAMA
                     LIRPEVIGAHMRFLADDLLEGRGTGTRGYDIAAQYVATQLEAAGLEPAGAGGTYFQPV
                     PFRRSVAEEKECSVTLQRQGSKADLRYGVDYIMRVDSLLPESAEVTAPVAFVGFGVTA
                     PELNYDDYAGVDVRGKIVALLTDAPAAFPHNQRAYYSSTRLKGENAVAHGAIGVFIIS
                     NRDDRKSFPWSQVVRHAKTGGMRWVDAAGKPDEVWGEIRGSAWLSEQGAAALFQGAPK
                     TIDQALDDADASKPQGFDLPVRATLKSTARHTRVDSPNVAAVLRGSDPRLRDQYVVYT
                     GHLDHVGVSDPVDGDSINNGAFDNASGIAALIEAAKAFVSLPTAPRRSILFVAVTGEE
                     KGLRGSDYFAQHPTVPRERIVANVNLDMFVMPFPLKDVVAFGAEHSSLGPVVEAAAKR
                     VGFTLSPDPAPEEVIFIRSDQYSFVRQGVPAVFITHGAQSADPKIDGPARIVEWMRTR
                     YHRPKDDMDQPMHLESGVKFARLNFLIGYDVAQADQPPTWNPGDFFG"
     gene            1780..2778
                     /locus_tag="VEL74_15100"
     CDS             1780..2778
                     /locus_tag="VEL74_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYG63903.1"
                     /translation="MKNVALQKLGLGGERGEISGLQAVTPDLALLQLSIVNVLFHGRP
                     GAGDRKWVLIDTGLTTSAPRIIRAASEWFGVGARPSCIVLTHGHFDHVGSLRELAEMW
                     DVPVYAHPLEMPYLTGRSDYPPPDPTVGGGAMAYLSRFYPRKGIDLGGRVQALPADGS
                     VPDMPGWRWIHTPGHTPGHVALFRDSDRALIAGDAFVTQVQESATGVLTRRPVVHRPP
                     AYFTTDWWAARRSVEELAALQPEVAATGHGIPMHGEVLRRQLDELAMSFDDAVPAHGR
                     YVRHPALADETGVVALPPPVPDRMPAALFALGVLAVAGFALSRRRHAVVHTGSDVW"
     gene            complement(2780..3604)
                     /locus_tag="VEL74_15105"
     CDS             complement(2780..3604)
                     /locus_tag="VEL74_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018348086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HYG63904.1"
                     /translation="MPFGLPDSEQRLSYGGYLKVGELTSLQHLLSDPPHHDEMLFIII
                     HQVYELWFKQILHEVDAAIVRLDQGDVLGATRVFRRCFEIQRLLVGQIDVLETMTPMD
                     FLAFRDHLFPASGFQSSQFREIEYVSGLKDPRFLENYPPGTAERARLEERLAGPTLRG
                     AFYDLLRRRGFDLPEEEEGREARVRELARLYREVEQHYDLFLLAEALIGYDEGFGLWR
                     LRHVQMVERIIGGRPGTGGSEGAQYLRSTTDRRFFPDLWDVRSELGVPPSVGHGKM"
     gene            3719..4138
                     /locus_tag="VEL74_15110"
     CDS             3719..4138
                     /locus_tag="VEL74_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63905.1"
                     /translation="MAADPQLPTTTTPARRRGCGKPVLVLLLLVLIAAGIFALYSWIT
                     LSYSYSDGERVGYVQKFSHKGWLCKTWEGELQIVSMPGSMPQIFAFSVRDEAVAERIN
                     KALGSRVALHYEEHRGVPTSCFGETGHFVTDVRVVGQ"
     gene            complement(4144..4554)
                     /locus_tag="VEL74_15115"
     CDS             complement(4144..4554)
                     /locus_tag="VEL74_15115"
                     /inference="COORDINATES: protein motif:HMM:NF018826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HYG63906.1"
                     /translation="MEARTRETIRECARLSFAGEITFPEVVRRLAAEGVERYHADLVG
                     RETSYYDATERVEKKALPLEGAPAIAERFSEEGVRQALAAVQGREIGYPDFLRRIMAA
                     GTVAYTVFFKGRHVLYAGRDGGFHVERFPGAPPH"
     gene            complement(4644..5423)
                     /locus_tag="VEL74_15120"
     CDS             complement(4644..5423)
                     /locus_tag="VEL74_15120"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HYG63907.1"
                     /translation="MTRVHPALAPYVKEILVEDPAGAPPAPRPYEVLPKPFPVIGFQY
                     RGRLEVLRDDGPELLARGGITGLQATRRSFRPGPETRSVLVVLEPHGAWPLLGCPMSE
                     VAGGHVPLGSILPAAPTRDLEDRLQEAGGIDELGELVQSFFLDLLDRSRRRPHPAVLD
                     AVGRIRQAHGTLPVEAVARGLGISRRQMERLFKLQVGVGPKELASLTRFEWVLGQLPR
                     RRSWADLAFGAGYADQSHFVRSFTARTGRPPGDFAAALEGV"
     gene            5422..6141
                     /locus_tag="VEL74_15125"
     CDS             5422..6141
                     /locus_tag="VEL74_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63908.1"
                     /translation="MAAQGSTVRDRHAGEMNLPGLLVPLRASAQGPAASLVRDLNTRV
                     VERSPFEPVLGSYPDAFTSLGGKLFFTAQAGQPGDSTGVWVSDGSASGTRQLTSSPSE
                     IVGAAAPPPARVTERTGLPAGSAAAGACVPAAGRLCLNGGRFAVEARWKDFAGQTGSG
                     TGVGLTGDTGYFWFFGPENVEVMVKVLDDQALNGKFWVFFGALSNVEYTLTVTDTLTG
                     KVKSYTNPGGRFASVGDTGAF"
     gene            complement(6155..6640)
                     /locus_tag="VEL74_15130"
     CDS             complement(6155..6640)
                     /locus_tag="VEL74_15130"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYG63909.1"
                     /translation="MNKVAELIEEFGRIYSGDPWYGPSMREVLTGISAAEAAVHPLPA
                     AHSVWDLVLHMTGWTEEVARRVEGGEPSLPQAGDWPALPVVSAATWDAALAGLESAHE
                     RLLRALEGFPEERLSEMVGDARDLPLGTGVSFEGMLRGLVQHHAYHTGQIALLKKALK
                     E"
     gene            6755..>7572
                     /locus_tag="VEL74_15135"
     CDS             6755..>7572
                     /locus_tag="VEL74_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63910.1"
                     /translation="MRDLRSLPLLLALLTVPVLSHAQLPPAATGVARNVPDGFEVRWH
                     IVRPGETLRGITEKYLGSSARWPENHRLNPGVADPDRLLPGQRLRVLLPKRGAPPAAQ
                     VRKVSRRVEAKPSPTPWSEAWIDDLLLERDGLRTFTKSSAEMEFTDGTRLLVTEDSLV
                     FLSRAGGAGGAGGRLRGVDRKAVEIVEGQADVEARPAAGRPTPEVEIVLGTTRATAKP
                     GPAGTSQSRARKAGPGAKVMVYGGTGEVEAGGAKVAVPEGMGTSVAQSGPPSPP"
BASE COUNT         1066 a         2750 c         2674 g         1082 t
ORIGIN      
        1 gccgaagaag tcccccgggt tccaggtggg cggctggtcc gcctgggcca cgtcgtagcc
       61 gatgaggaag ttgagccgcg cgaacttgac cccggactcc aggtgcatcg gctggtccat
      121 gtcgtccttg ggccggtgat accgggtgcg catccactcg acgatgcggg cggggccgtc
      181 gatcttgggg tcggcgctct gggctccgtg ggtgatgaag accgccggca ccccctggcg
      241 cacgaaggag tactggtcgc tgcggatgaa gatgacctcc tccggcgccg ggtccgggct
      301 caacgtgaag cccaccctct tggccgcggc ctcgaccacc ggcccgaggc tggagtgctc
      361 ggcgccgaag gccaccacgt ccttcagcgg gaagggcatc acgaacatgt ccaggttgac
      421 gttggcgacg atgcgctccc gcggcacggt ggggtgctgg gcgaagtagt cggagccccg
      481 cagccccttc tcctcgccgg tgacggcgac gaagaggatc gagcgccgcg gcgccgtggg
      541 cagggagacg aacgccttgg cggcctcgat cagggcggcg atgcccgagg cattgtcgaa
      601 ggcgccgttg ttgatggagt cgccgtccac cggatcgctc acccccacgt gatcgaggtg
      661 gccggtgtag accacgtact ggtcgcgcag ccgcggatcg gagccgcgca gcacggccgc
      721 gacgttgggg ctgtccaccc gggtgtgccg ggcggtgctc ttgagcgtcg cgcgcaccgg
      781 caggtcgaag ccctgcggct tgctcgcgtc ggcgtcgtcg agggcctggt cgatcgtctt
      841 cggcgccccc tggaagaggg ccgcggcccc ctgctcgctc agccaggcgg agccccggat
      901 ctccccccag acctcgtccg gcttgccggc cgcgtccacc cagcgcatgc cacccgtctt
      961 ggcgtgccgc acgacctggc tccacgggaa gctcttccgg tcgtcccggt tggagatgat
     1021 gaagacgccg atggcgccgt gggcgacggc gttctccccc ttgagccggg tcgacgagta
     1081 gtaggcccgc tggttgtgcg ggaaggcggc cggcgcgtcg gtgagcaggg cgacgatctt
     1141 gccccggacg tccacgccgg cgtagtcgtc gtagttgagc tccggcgcgg tgacgccgaa
     1201 gccgacgaag gcgacggggg cggtcacctc ggcgctttcg ggcagcagac tgtccacccg
     1261 catgatgtag tccacgccgt agcggaggtc cgccttcgac ccctggcgct ggagggtgac
     1321 ggagcactcc ttctcctccg ccaccgacct gcggaacggc accggctgga agtaggtgcc
     1381 gccggccccg gccggctcga gaccggcggc ctcgagctgc gtcgccacgt actgggccgc
     1441 gatgtcgtac ccgcgggtgc cggtgccgcg cccctccagg aggtcgtcgg ccaggaaccg
     1501 catgtgggcc ccgatgacct ccgggcggat cagcgccatc gcctggtcgg taaccgccgc
     1561 gtcggtgatc tggggattga ccgcgggggg ctgcgcggcg gcgccgcagg cgagggtgaa
     1621 aagggctgcg gccgtccaga ggggccgcga aacaggacgg tgcatcggag ggctcctttc
     1681 gttctctggc ccgtggagtg tacccgatgg cacccccgtt gcactttctt cccgaggggg
     1741 ccatctcgtc cccgctacgt cactcccgga ggtaccaaca tgaaaaacgt agccttacag
     1801 aagctcggtc tcggcggaga gcgcggggag atcagcgggc tccaggcggt gacgccggac
     1861 ctcgcccttc tccagctctc gatcgtcaac gtcctcttcc atggccggcc cggcgccggc
     1921 gaccggaaat gggtcctgat cgacacgggc ctcaccacct cggcgccgcg gatcatccgg
     1981 gcggcttccg aatggttcgg cgtgggggcg cgcccctcgt gcatcgtcct gacgcacggc
     2041 cacttcgatc acgtgggatc gctgcgcgag ctggccgaga tgtgggacgt cccggtctac
     2101 gcgcaccccc tggagatgcc ctacctcacc ggccgctccg actatccgcc cccggatccg
     2161 acggtcggcg gcggcgccat ggcctacctg tcgcgcttct atccccgcaa ggggatcgac
     2221 ctcggcggcc gggtccaggc cctgccggcc gacggcagcg tgccggacat gccgggctgg
     2281 cgctggatcc acacgccggg gcacaccccc gggcacgtgg cgctcttccg ggattcggac
     2341 cgggcgctga tcgccggcga cgccttcgtc acccaggtcc aggagtcggc taccggcgtg
     2401 ctgacgcgcc ggccggtggt gcaccggccc cccgcctact tcaccaccga ctggtgggcc
     2461 gcccgccgct cggtcgagga gctggcggcg ctgcagccgg aggtggcggc caccgggcac
     2521 ggcatcccca tgcacggcga ggtgctgcgc cggcagctcg acgagctggc gatgagcttc
     2581 gacgacgcgg tccccgcgca cggacgctac gtccgccacc cggcgctggc cgacgagacc
     2641 ggcgtggtcg ccctgccgcc gccggtgccg gaccgcatgc ccgcggcgct gttcgccctc
     2701 ggcgtcctgg ccgtggccgg cttcgcgctc agccgcagga ggcacgccgt ggtccacacc
     2761 gggagcgacg tctggtgatc tacatcttgc cgtgaccgac ggagggaggc acgccgagct
     2821 cgctccgcac gtcccagagg tcggggaaga aacggcggtc ggtggtggag cgcaggtact
     2881 gcgccccctc gctgccgccg gtccccggcc ggccgccgat gatccgctcg accatctgca
     2941 cgtgccgcaa ccgccagagc ccgaacccct cgtcgtagcc gatcagcgcc tcggccagga
     3001 ggaagaggtc gtagtgctgc tcgacctcgc ggtagaggcg ggcgagctcg cggacccggg
     3061 cttcccggcc ctcctcctct tccggaaggt cgaagccgcg gcgccgcagc aggtcgtaga
     3121 aggcgccgcg gagggtcggc ccggcgaggc gctcctccag gcgggcgcgc tccgctgtcc
     3181 ccggcgggta gttctccagg aagcgcgggt ccttgagccc ggagacgtac tcgatctccc
     3241 gaaactgcga cgactggaag ccgctcgccg ggaacaggtg gtcgcggaag gccaggaagt
     3301 ccatgggggt catggtctcc agcacgtcga tctgccccac cagcaggcgc tgaatctcga
     3361 agcagcgccg gaagacccgg gtcgccccca gcacgtcccc ctggtcgagg cggacgatgg
     3421 cggcgtcgac ctcgtgcagg atctgcttga accagagctc gtagacctgg tggatgatga
     3481 tgaacagcat ctcatcgtgg tgcggcgggt cggagagcag gtgctggagg gaggtgagct
     3541 cccccacttt caggtacccg ccatagctca ggcgttgctc gctgtccggc aatccaaacg
     3601 gcatcagagg cctcccatgg tttccggcgc aggagtctag catccgggcg gagcgcttcc
     3661 acgggcatcg cctctgctga tagtctctgg tctgtctcgg cagtacggag ggaacccgat
     3721 ggccgccgat ccccagctcc ccacgaccac cacccccgcc cgccgccgcg ggtgtggcaa
     3781 gcccgtgctc gtcctcctgc tcctggtgct gatcgcggcc gggatcttcg ccctgtactc
     3841 ctggatcacc ctgagctaca gctactcgga cggcgagcgg gtcggctacg tccagaagtt
     3901 ctcccacaag ggctggctct gcaagacctg ggaaggggag ctccagatcg tcagcatgcc
     3961 gggcagcatg ccgcagatct tcgccttctc cgtccgcgac gaagccgtcg ccgagcgcat
     4021 caacaaggcg ctggggagcc gggtggccct ccactacgag gagcaccgcg gcgtccccac
     4081 ctcctgcttc ggcgaaaccg gtcacttcgt caccgacgtg cgggtcgtcg gccaataaga
     4141 cggtcagtgc ggcggcgcgc ccgggaagcg ctcgacgtgg aagccgccgt cgcggccggc
     4201 atagaggacg tgacggccct tgaagaagac ggtgtaggcc acggtacccg ccgccatgat
     4261 ccggcgcagg aagtccggat agccgatctc ccttccttga acggcggcca gggcctgccg
     4321 caccccctcc tccgagaagc gctccgcgat cgcgggcgcc ccttctaaag gaagcgcctt
     4381 cttctccacc cgctccgtgg cgtcgtagta gctcgtttcg cgacccacga gatcggcgtg
     4441 gtagcgctcc accccctcgg cggccagccg ccgcaccacc tccgggaacg tgatctcccc
     4501 cgcgaacgac agccgcgcgc attcgcggat cgtctccctg gtcctcgctt ccatgactcc
     4561 tcctttcgca acgggcgccg gtgcccgccg tcgagaggca tcctaggaag gagggagggg
     4621 cccggtcttg aagaaatgcg acgtcagacg ccttcgagcg ccgcggcgaa gtcgccgggg
     4681 ggccggcccg tccgtgccgt gaagctgcgg acgaagtggg actggtccgc gtagcccgcg
     4741 ccgaaggcca ggtccgccca ggaccggcgg cgcgggagct gtcccaggac ccactcgaac
     4801 cgggtgaggg aggcgagctc cttgggcccg acgccgacct ggagcttgaa gagccgctcc
     4861 atctgccggc ggctgatccc caggccgcgg gcgacggctt ccaccgggag ggtgccgtga
     4921 gcctggcgga tccgccccac ggcgtcgaga accgccgggt ggggccggcg gcgcgaccgg
     4981 tccagcaggt cgaggaagaa ggactgcacc agctccccca gctcatctat cccaccagcc
     5041 tcctggagcc ggtcctccag atcccgcgtt ggggcggccg ggaggatcga gccgaggggg
     5101 acgtggccgc cggcgacctc gctcatcggg cagccgagca ggggccaggc gccgtggggc
     5161 tcgagcacca cgaggaccga gcgggtctcc ggcccgggcc ggaaggaacg ccgcgtcgcc
     5221 tggaggccgg tgatgccgcc gcgcgccagc agctccggcc cgtcgtcgcg cagcacctcc
     5281 agccggccgc ggtactggaa gccgatgacg gggaacggct tcggcaggac ctcgtacggc
     5341 cgcggcgccg gcggagcgcc ggccgggtcc tcgaccagga tctccttcac gtagggcgcg
     5401 agggccgggt ggacacgggt catggccgcc cagggtagca ccgtccggga ccgtcatgcc
     5461 ggggagatga acctgcccgg tctcctcgtg ccgcttcgcg cgtccgccca gggtccggcg
     5521 gcctctcttg tccgggacct caacacccgg gtggtcgagc ggtccccctt cgagccggtc
     5581 ctcgggtcct atccggacgc gttcaccagc ctcgggggca agctgttctt caccgcccag
     5641 gcgggacagc caggggactc caccggcgtc tgggtcagcg acggctccgc cagcggcacc
     5701 cggcagctga cctcctcccc gagcgagatc gtgggcgcgg ccgccccgcc gcccgcccgg
     5761 gtcaccgagc ggaccggcct ccccgccggc agcgccgccg ccggcgcctg cgtgcccgcg
     5821 gccggccggc tgtgcctcaa cggcggccgc ttcgccgtcg aggcccggtg gaaggacttc
     5881 gccggccaga ccggcagcgg caccggcgtc ggcctcaccg gcgacaccgg ctacttctgg
     5941 ttcttcggcc cggagaacgt cgaggtgatg gtcaaggtgc tcgacgacca agcgctgaac
     6001 ggcaagttct gggtcttctt cggcgctctg tcgaacgtcg agtacaccct gaccgtcacc
     6061 gacacgctga ccggcaaggt gaagtcctac accaacccgg gcggacgctt cgccagcgtg
     6121 ggcgacaccg gcgcgttctg agaacggcgg atcgtcactc cttgagcgcc ttcttgagga
     6181 gggcgatctg cccggtgtgg taggcgtggt gctggacgag gccgcgcagc atcccctcga
     6241 aggagacgcc ggtgcccagc ggcaggtcgc gggcgtctcc caccatctcg gacaggcgct
     6301 cctcggggaa gccctcgagg gcgcgcagca gcctctcgtg ggcgctctcc agcccggcca
     6361 gggcggcgtc ccaggttgcc gcgctcacca ccgggagcgc cggccagtcc ccggcctgcg
     6421 gcagcgacgg ctcgccgcct tcgacacgcc gggcgacctc ctccgtccag cccgtcatgt
     6481 ggaggacgag gtcccagacc gagtgcgcgg cgggcagcgg gtggaccgcc gcctccgccg
     6541 ccgagatgcc ggtgagcacc tcgcgcatgg acggcccata ccagggatca ccgctgtaga
     6601 tccgtccgaa ctcctcgatc agctctgcca ccttgttcat gagcgcctct ctttcagggg
     6661 aacccacccg gaccgcttgc ggggcccggc ctggaatcgt acagtagccg ggcccgtcca
     6721 gccgagaccg ctttcgatct aggagaccac ctcgatgagg gacctgcgct cgctgccgct
     6781 gctgctcgcc ctgctcaccg tgcccgtcct gtcgcacgcc cagttgcccc cggcggcgac
     6841 gggggtggcc cggaacgtgc ccgacggctt cgaggtgcgc tggcacatcg tccgccccgg
     6901 cgaaaccctg cgcgggatca ccgagaagta cctgggctcg tccgcccgct ggccggagaa
     6961 ccatcgcctg aaccccggcg tcgccgatcc ggaccgcctc ctccccggcc aacggctccg
     7021 ggtgctcctc cccaagcgcg gcgccccgcc ggcggcgcag gtccgcaagg tgtcgcgccg
     7081 ggtggaggcg aagccctccc ccaccccctg gagcgaggcg tggatcgacg acctgctgct
     7141 ggagcgcgac ggtctgcgca ccttcaccaa gtcctccgcc gagatggagt tcaccgacgg
     7201 cacccgcctc ctggtgaccg aggactccct ggtcttcctg agccgcgccg ggggcgccgg
     7261 cggcgccggg ggtcgattgc gcggcgtcga ccggaaggcg gtcgagatcg tggaggggca
     7321 ggcggacgtc gaggcccgcc cggcggccgg ccgccctacg cccgaggtcg agatcgtcct
     7381 cggcaccacc cgcgcaaccg cgaagcccgg ccccgccggc acctcacaga gccgcgcccg
     7441 gaaggcagga ccgggcgcca aggtgatggt ctacggcggc accggcgagg tggaggccgg
     7501 cggcgccaag gtggccgtgc ccgagggcat ggggacgtcg gtggcgcaga gcgggccacc
     7561 gtcgccgccg ga
//
LOCUS       DASPJI010000550        15156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_602891, whole genome shotgun sequence.
ACCESSION   DASPJI010000550 DASPJI010000000
VERSION     DASPJI010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15156)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15156
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602891"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /locus_tag="VEL74_15140"
     CDS             complement(<1..1282)
                     /locus_tag="VEL74_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63911.1"
                     /translation="MLVDPARSSRQAILSAVLLVLGGIALAYTLARAALLSFTHDESV
                     TYLHFVDAPLLETLRYNGFSPSNNHLLNSLLMRMSATLFGPGELALRLPSWLGHVLYV
                     AAAVLLVRRMRHPLAAVCTFVLLLANPFLLDFFSLARGYGLAMGLMLAGVYVLVTTLE
                     EAAPRPVRAVAAFALLAGAALAHFGFLNVYLAAAAVLLGFALQRRAMRTAGAVAVLSG
                     LFVAGVLPTLLKLKNGGELYHGGEVGFVADTVRSLVESALYGEHPLHRPGRPMALAVA
                     ALVLFLASALVVWTRRRRPAAGAVDDGAVVSAIAVVTAVGILLQHHLFGTRFVIDRSA
                     LFFLPLFFLTLGAAVDREIEAGGRMGRTLFAGAYGVLSLLLLVHTAGRANLTHTDAWR
                     YDADTERMIEALAARAPSKERPRKTLGVFWAYEPA"
     gene            1453..2430
                     /locus_tag="VEL74_15145"
     CDS             1453..2430
                     /locus_tag="VEL74_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63912.1"
                     /translation="MRSKSLTLGLLVLVACSGASWAAGAVEGLRTPAELTFGNRPAAQ
                     APTAPLTRTLFVRVPGKAGRAERSLEVRVQFNGRPYLTEKIVVPASRQDLAIELLARD
                     PKALEGILGLADREVGAITVALHLDGKALHEMTLQELERASVRLRGEDGFRPVPAASK
                     VELLIPAPPAPDFLKPAMDKGYQPDPECEQQCHDTYVTCYYEICDQRGDCSYCWEDYE
                     YCDDMCPQICVDPKSVQTFTTTQVIGVTTYWSTCYERPWENDFTWGRWYDYRRYDYKH
                     TQIRRTTNCDNSYTDEVLSVSYSSDYCNYETFSSCYQPSSKVSSWDVCF"
     gene            complement(2477..3184)
                     /locus_tag="VEL74_15150"
     CDS             complement(2477..3184)
                     /locus_tag="VEL74_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HYG63913.1"
                     /translation="MALNSAIEWTESTWNPVTGCTKISPGCKFCYAERMAKRLRAMGQ
                     GNYRNGFELTLQPQSLEAPLTWKRPQTIFVNSMSDLFHRDVELEYIQKVFSVMEQASW
                     HRFQILTKRAERLSEVAPHLPWPANVWMGVSVESSDYTFRIDHLRSTQARTKFLSLEP
                     LLGPLPRLDLRGIAWAIVGGESGPGARPMDPAWVTAIRDQCRTAGVAFFFKQWGGVHK
                     KRTGRLLEGRTWDEMPA"
     gene            complement(3184..4092)
                     /locus_tag="VEL74_15155"
     CDS             complement(3184..4092)
                     /locus_tag="VEL74_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009786698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HYG63914.1"
                     /translation="MTKRKEGAEPLEHHRFGGDWTTTKLEILKHYLRGYTTALKKTPF
                     KTAYIDAFAGTGYRTLRQKDFGESPLYPDLIEAAPQALLDGSARMALQVEPPFNRYIF
                     VEKSHEHCDQLLQLKEEFPELAKDIRIEEAEANEYIQKLCGKKGWGKHRAVLFLDPYG
                     MQVEWETIEAVARTKAIDLWVLFPLGIGVNRLLTRSGDIPESWKRRLDLLLGTQDWYD
                     EFYKVQSTPTLFGPQETPVKESLETIGRFFNERLKTIFAGVAPTPKVLRNSANCPLYL
                     FCFAVGNKAGAPIALEIANHLLRKID"
     gene            4292..4582
                     /locus_tag="VEL74_15160"
     CDS             4292..4582
                     /locus_tag="VEL74_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYG63915.1"
                     /translation="MNRNDVLTILEAHHADLDRFGVKTLRLFGSVARGEASPGSDVDL
                     LVGFRSVPTFSEFMKLRIFLEDRLGVKVDLVTESGLRESVRPSVERDAIRVA"
     gene            4696..6600
                     /gene="asnB"
                     /locus_tag="VEL74_15165"
     CDS             4696..6600
                     /gene="asnB"
                     /locus_tag="VEL74_15165"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HYG63916.1"
                     /translation="MCGIAGIFRFRSGRPVDPGELRAMGACLAHRGPDDEGYYNDGPL
                     GLAHKRLTILDTSRRGRGPMFTDDGRVVITFNGEIYNFLELRADLEKKGYTFHTRTDT
                     EVILRLWQEHGEGLLEHLNGMFAFAIWDLDRRKLFLARDRLGIKPLYYQVTGEGIAFA
                     SEVKALLAAAGRRPGVDLEMLDAYMSVGYVPTERTLFAGVTKLQPGWCLTVHDGRIDL
                     RQYWDLRFETEETPEITEEECVRQATDLLRDAVRLQLRSDVPLGVFLSGGVDSSAVVA
                     LMHDLGIRDIRTFTVAYDFGPGFDETRWARQIAQQFGTIHREVFVTPQDFQDFIPSLV
                     WHMDEPVTESAAISLYFVSKLAREDVVVVLSGEGSDEVFGGYPIYKYMQTLERYRRLP
                     GALRRGVINPALNLLGGKWPKYTQLAEQALPERYLGVSFYETPVKNSLLRPEIRSLFN
                     GHAMSSVARRYYEKTEGLDPLLRMMYLDVKSWLPDDLLIKADKMTMASSVELRVPFLD
                     HRVVELGARIPPRYRIKGWSTKHILKKAMEPYLPHEILHRGKMGFPTPLARMFQREMK
                     PYVADVLTSDRFFDRGYFQPDVVRSMVTEHTAGEKDHHRVLWQLLVLEEWHRRFVDGE
                     ERGAAGAPRG"
     gene            complement(6606..7928)
                     /locus_tag="VEL74_15170"
     CDS             complement(6606..7928)
                     /locus_tag="VEL74_15170"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG63917.1"
                     /translation="MKKRLLDWIVCPGCGERPQLQVFQEERVELPAPVQPPACSSYCG
                     RHGIASPRVITPPPDCNSCYREEILEGALSCACGLVYPVLQGVPRMIRNAREEYPEFF
                     ARHAHQLGRPLGEAAALPAAPAPAPAAAPQPQAAAQPQAAAPVVDGRSPRSFGLQWAI
                     YREGDHTWFKDDANLRKQEFLYNLDTTPGELASSTLLDGGCGNGELTRSVAEYGPEIV
                     AMDLSRSVEGARQRLFEKGFAVSHRVHYLQGNLLELPLLPRSFDMVHSSGVLHHTPST
                     YRAFRSVSKAVRPGGKLYIQLYRRRPTWIHLINVALRAVTTRMPMNVLYALCYATTPV
                     HAALSRLMHALRREPAPPRATARERAVQMFDNYSPRYQYRHTVPEIMALFRSEGYEDL
                     KDVTLDNEARHMLAVLGRRAAGEAKMAAPAAEPVRLASPASPAQPQTV"
     gene            8135..8731
                     /locus_tag="VEL74_15175"
     CDS             8135..8731
                     /locus_tag="VEL74_15175"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HYG63918.1"
                     /translation="MEIKQHFYRWLLCALVGAVVAVPVVADVVVRTEEDAPNAYAIGI
                     GDVVEISVWKNPDLTTTVPVRPDGRISVPLLGDLQAAGMTPLALRQTITDRYKEFVTA
                     PGVSVVIKEINSRKVFVTGEVATPGAYDLPPRAKLMQLLAMAGGLTPYAKGRVVVLRD
                     RDGDGKDRRYEIQLDTIISGRKPGDNLVLQPGDTLVVP"
     gene            9295..10689
                     /locus_tag="VEL74_15180"
     CDS             9295..10689
                     /locus_tag="VEL74_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63919.1"
                     /translation="MVGSSTSTKVGAALRLPALFALLLAGMPAGAGELRLVIEGEAGV
                     RNDGNLEQRAQIEATGSGDEEDRNVARGGLSFQLSYDLPRADLALIYSPSYEESIDDS
                     EISGTTHRLDLGYEGELTRRLRLRVRERLLKSPNLDLYAPLSVAEPVAVTRRGDQLSH
                     GLDVGFEHDLTRRAVLVVGAQHSLRTFDGEELEDAPLYDTETLVGTLGATFSLAEDRQ
                     LGVSASAGEYDYERRGEADVQNVGVSYSQGWGRSGRVSVEAGLFSVESTLLRRGVIAL
                     PDAPDEAPGPPVVEEVLTEEEDEGWRGAVSVSRQGRFIGWNLGYRHDVSAGFGLGEPT
                     EADSAFAGLSYSPTRRLTLGLDGNASRHEPLGDSERAVGTLLQRQDTLNEFAAGTARL
                     SWTFFQGLRLTGGYSRIWQSSEVEPFEDLSYDRFFLGLAFRVWSSGETPREPDDPQGE
                     LTDEARDDQEPDAR"
     gene            10652..12148
                     /locus_tag="VEL74_15185"
     CDS             10652..12148
                     /locus_tag="VEL74_15185"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HYG63920.1"
                     /translation="MKHATIKNLTPAELGKSVLRRPGWFLVPVLLGLVGAFLALRMLT
                     PIYRASTQVLVEPQKVPADYVKPTVTTDIDERLKTLEQQITNRSHIERIIQEMNLYPE
                     LRRELAMEKLVDQVRRKDLTVERRGDIFSIGFKSQDPKKAAATANRIAELFIQENLKL
                     RENQAQGTSSFLSAELQQAKEKLEQQEARVAVFKQLHMGQLPEQRDTNLQQVGQLQDK
                     LEINMDALDRAETRKILLQSQVAEARRQAQMPRQPVQPTILMTTPGAPSRLDQLRAEL
                     IDLRSRFTDRHPDVIRLQNQIAQLERAASEAPPPAPPPQTMASREPEPVRIDPELLAQ
                     LQSVDYEIRNLHAERGRILADISRVQARLEEIPRVEQELLSLTRDYENKQRTYESLLE
                     KQSQARLAENLEKRRQSEQFTILEKAVPPAEPFFPNPLYVLAAGLAAGALAGILLSLL
                     RDQTDSTYADGDALQQAFPGVRVLATIPIFSAEAVAGSATAQSRFRRN"
     gene            12188..12883
                     /locus_tag="VEL74_15190"
     CDS             12188..12883
                     /locus_tag="VEL74_15190"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HYG63921.1"
                     /translation="MATPTETIRLREDAPVILHVDPPSFPAEQYRLLAVQVEERLNPI
                     GTWGFALSVTSPDEGVGKTLTSLNLALTLTRGEERRVLLLEGDLWRPRLHTYFDPEDV
                     NRPGLQQVLERRIQLSEAVVAVEGTSLDLLPAGAAGFAGDVMSGRRMSEVLTEARAAY
                     EIVVIDSPPMGMLASARSLASRADGVVLVVRAGQSKRRSIEKTLEGLGPDKLIGMVLN
                     GIKTPGRGYRGYY"
     gene            12917..14311
                     /locus_tag="VEL74_15195"
     CDS             12917..14311
                     /locus_tag="VEL74_15195"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HYG63922.1"
                     /translation="MIRLFNRYWTISAFISLAVEGSILFLSVPMATWIRLGLGPGDPP
                     PRSIQALEGLAFMVVGLLALYINGLYDFGERQSSRHMFIRLLRAGCLAGVGLMAVYFL
                     FPKFLTGRGIFAISFVVAAWGVTLWRLVLRALLSSDRFAERVLIVGSGEKAIDIAREI
                     LQRKHLGFRVVGFLDNDPKLQGVSLINPRVIGTTDQCRDLAMLHGVTRVVVAANDFRG
                     GQVSLDALLACKTSGIKVQEASSYYEQVTGKIMLEGLRKSWLVFSDGFVVSRGALFAK
                     RVLDLAAATTGLILAAPVMLLVALAVRLDSPGPVFFRQDRVGRGGREFTLWKFRSMRT
                     DAEAGGAQWAVQGDPRVTRVGRFIRKTRLDELPQLWNVLVGDMSLVGPRPERRMFVDQ
                     LKEQIPLYEQRLVVRPGLTGWAQIKAPYASSFEESLEKLKFDLYYIKNLSLFLDISIL
                     LSTARTVLLGRGAR"
     gene            14308..>15156
                     /locus_tag="VEL74_15200"
     CDS             14308..>15156
                     /locus_tag="VEL74_15200"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG63923.1"
                     /translation="MTDSADRPKPPAPLLRHGTRPSAALRILYLCHRVPYPPDKGERI
                     RAFHQIRALSRRHRVSLFTLADSAVPDLRPLEEMCERVEVFNLNRPAAYLRSALSVWR
                     RRPLTLSFFESAELKTRLQALAKSERFDVVVVYSSAMAPYADALPGVPAVLDMVDVDS
                     AKWAQYSSFAKRPMRPVYALEARRLQAYEASLVDRFQRVVLVTDGEARTLRSFAPEAR
                     PESIPNGVDFDFFRALDLPKAELPTLVFTGQMDYFANIDGVCHFAREVFPRLRARYPD
                     LQFAVVG"
BASE COUNT         2412 a         5083 c         5153 g         2508 t
ORIGIN      
        1 ccgccggctc gtacgcccag aacacgccca gcgtcttgcg gggccgctcc ttggagggtg
       61 cccgtgctgc cagcgcctcg atcatccgct cggtgtccgc gtcgtagcgc caggcgtcgg
      121 tgtgggtgag gttggcgcgg cccgctgtat ggacgaggag caggagggag aggacgccgt
      181 aggcccccgc gaagagggtg cgccccatcc ggccgcccgc ctcgatctcc cggtccaccg
      241 ccgctcccag ggtcaggaag aagaggggga ggaagaagag ggcggagcgg tcgatcacga
      301 accgggtgcc gaagaggtgg tgctggagga ggatgccgac ggccgtcacc acggcgatgg
      361 cggagacgac ggcgccgtca tccaccgcgc ccgcggccgg ccgccggcgg cgcgtccaga
      421 ccacgagggc gctggccagg aagagcacga gcgcggccac cgcgagcgcc atgggccggc
      481 cggggcggtg gagcgggtgc tcgccataga gggcgctctc gaccaggctg cggacggtgt
      541 cggcgacgaa gcccacctcg ccgccatggt aaagctcgcc tccattcttg agcttgagga
      601 gggtcggcag caccccggcg acgaagaggc cgctgagcac cgccacggcc ccggcggtcc
      661 tcatcgcccg ccgctggagc gcgaagccca ggagcaccgc cgccgcggcg agatagacgt
      721 tgaggaagcc gaaatgcgcc agcgcggcgc cggcgaggag ggcgaacgcg gccacggccc
      781 ggacggggcg cggcgcggcc tcctccagcg tggtgaccag cacgtagacg ccggccagca
      841 tgagccccat ggccagccca tagccccggg cgagggaaaa gaagtccagc aggaacgggt
      901 tggccaggag cagcacgaac gtgcagacgg cggccagcgg gtggcgcatc cgtcgcacca
      961 ggaggacggc cgcggctacg tacaggacat gccccagcca gctcggcagc cgcagcgcca
     1021 gctcacccgg gccgaacagc gtggcgctca tccgcatcag cagcgagttc agcaggtggt
     1081 tgttcgacgg agagaagccg ttgtacctga gagtttccag gagaggcgcg tcgacgaagt
     1141 ggagataggt gacgctctcg tcgtgggtga acgagaggag cgccgcgcgg gcgagcgtat
     1201 aggccagggc gatgccgccc aggacgagca ggactgcgga caggatcgcc tgccgcgagg
     1261 agcgggccgg atcgacgagc atgcgcgcgt ggagaattca tgcaacttgc gcgccgtccg
     1321 aggccggtcc gcgtctttgg accgggtgcc gaaccgtgcc cgcatttggc gcatctgtag
     1381 gtagaggcaa gccatgcccg ttcgctctgt ccagcccaat cccgggctgg gcgctcgttg
     1441 ggagatgaag tcatgcgttc gaaatctctg acattgggcc tgctcgtgct cgtcgcttgc
     1501 tcgggggcgt cctgggcggc cggagccgtg gaaggtctgc gcacgccggc cgagctgacc
     1561 ttcggcaacc ggccggcggc tcaggctccg acggctcccc tgacccgcac cctgttcgtg
     1621 cgcgtccctg gcaaggccgg tcgagccgag cgttcgctgg aggtccgggt gcagttcaac
     1681 ggccgcccct acctgaccga gaagatcgtg gtgccggctt cccgtcagga cctcgccatc
     1741 gagctcctgg cgcgcgaccc caaggccctg gaggggatcc tcggcctggc ggaccgcgag
     1801 gtgggcgcga tcacggtcgc gttgcacctg gacggcaagg cccttcacga gatgaccctc
     1861 caggagttgg agagggccag cgtcaggctg cgtggggagg acggcttccg tcccgtgccg
     1921 gccgcttcca aggtcgagct cctcatcccc gcgccgccgg cgcccgactt cctgaagccg
     1981 gcgatggaca aggggtacca gcccgatccg gagtgcgagc agcagtgcca tgacacctac
     2041 gtcacctgct actacgagat ctgcgaccag cggggggact gcagctactg ctgggaggac
     2101 tacgagtact gcgacgatat gtgtccgcag atctgcgtcg atcccaagag cgtgcagacg
     2161 ttcaccacca cgcaggtgat cggcgtcacc acctactggt ccacctgcta cgagcgtccc
     2221 tgggagaacg acttcacctg gggaaggtgg tacgactatc gccggtacga ctacaagcac
     2281 acgcagatcc ggcggaccac gaactgcgac aactcctaca ccgacgaggt gctgtccgtc
     2341 tcctactcga gcgactactg caactacgag acgttctcga gctgctatca accgagctcg
     2401 aaggtctcct cctgggacgt ctgcttctga ccgtccccgc gtcccccgcg gctccggctg
     2461 cggggggcgc ccctgctcag gccggcattt cgtcccaggt gcgcccctcc agcaggcgcc
     2521 cggtccgctt cttgtggacg cctccccatt gcttgaagaa gaaggcgacg ccggcggtcc
     2581 ggcactgatc ccggatcgcg gtgacccagg ccgggtccat cggtcgggcg cccggcccgg
     2641 attcaccacc gacaatggcc caggcaatgc ccctgagatc caggcgggga agcgggccca
     2701 gcagaggctc gagcgagagg aacttggttc tggcttgggt agagcggaga tggtcgatgc
     2761 ggaatgtgta gtcggagctt tcgacgctga cccccatcca gacgttggcc ggccagggga
     2821 ggtgcggggc cacctcggag agcctctccg ctctcttggt gaggatctgg aagcggtgcc
     2881 aggaagcctg ctccatgacc gagaacacct tctggatgta ctcgagctcg acatccctgt
     2941 gaaagaggtc gctcatggag ttgacgaaga tcgtctgcgg gcgcttccag gtgagggggg
     3001 cctccaggct ttgcggctgc agcgtcagct cgaagccgtt gcgatagttg ccctgtccca
     3061 tcgcccgtag gcgcttcgcc atgcgttccg catagcagaa tttgcatcct gggctgatct
     3121 tggtacagcc tgtgacggga ttccaggtgg attcggtcca ttcgatcgca gagttcaacg
     3181 ccatcagtcg atcttcctga gcaggtggtt ggctatttcg agcgcaatcg gggccccggc
     3241 cttatttccc acggcaaagc agaacaggta gagcgggcag ttcgctgaat tcctcagaac
     3301 ttttggggta ggggcgacac cagcaaaaat tgtcttgagt cgttcgttga agaagcggcc
     3361 aatggtctca aggctttcct tgaccggggt ctcctggggg ccgaagagag tcggagtgga
     3421 ttgaaccttg taaaactcgt cgtaccagtc ctgcgtgcca aggaggagat cgagccgccg
     3481 cttccaggat tcagggatgt ctcccgatct ggtgagcagc cggttcactc cgattccgag
     3541 cgggaagagg acccataggt cgatagcctt ggtcctggcg acagcttcga tcgtctccca
     3601 ctcgacctgc attccatagg ggtctagaaa cagaacggcg cggtgcttgc cccacccttt
     3661 cttcccgcag agcttctgga tgtactcgtt agcctctgct tcttcgatgc ggatgtcctt
     3721 tgccagctcc ggaaactcct ctttgagctg gaggagttgg tcgcagtgct cgtggctctt
     3781 ctctacgaag atgtagcgat tgaatggagg ctcgacctgt aaggccattc tcgccgatcc
     3841 gtccagcaac gcctgaggcg ctgcttcgat gagatctggg tatagagggc tctctccgaa
     3901 gtccttctgg cgaagtgtcc tgtagcctgt accggcgaag gcgtcgatgt aagccgtctt
     3961 gaaaggagtc ttcttgagag ccgttgtgta gcctctcagg tagtgcttga ggatttcgag
     4021 cttggtggta gtccagtcac ctccgaagcg gtgatgctcg agcggctctg cgccttcctt
     4081 gcgcttggtc atgtcctatc ttgcccttag gaagccgctt tgtccaccag ggagcgctag
     4141 cagcgttctg ggcctacccg ctgggttgct ctcagaagat atcatccctg ccgggtcgtc
     4201 cccccaagtt tttacagcgt tctcgccgcg acctgttacc attcataggg atcgtggtac
     4261 gctctattgc tgttatgctg gatggggctc catgaaccgc aacgacgtcc tgacgatcct
     4321 cgaagcgcac cacgccgatc tcgaccgatt cggtgtgaag actctgcgtc tcttcggctc
     4381 cgtggcccgc ggcgaagcct cacccgggag cgatgtggac ctcctcgtgg gattccggag
     4441 cgttcctacc ttctccgagt tcatgaagct gcggatcttc ctcgaggatc ggctgggtgt
     4501 gaaggtggac ctcgtcaccg agtccggtct ccgggagagc gtccggccat cggtggagag
     4561 ggacgcgatc cgtgtcgcgt aggtcctagg ctcccccctc tccggcccac tttttgcttc
     4621 cctctctccc tcaagaggtg accgccgatg gaatctgtgt ctccatcgcc tagagactga
     4681 cggaggagcg actgcatgtg tgggatcgcg ggcattttcc gcttccggtc gggacggccg
     4741 gtcgatccgg gggagctgcg ggccatgggg gcctgcctcg cccatcgtgg gccggatgac
     4801 gagggctact acaacgacgg tcccctgggg ctcgcgcaca agcggctgac catcctcgac
     4861 acctcgcggc gcggccgcgg ccccatgttc accgacgacg gccgggtggt catcaccttc
     4921 aacggcgaga tctacaactt cctcgagctg cgggccgacc tcgagaagaa gggctacacc
     4981 ttccacaccc gcaccgacac cgaggtcatc ctccggctct ggcaggagca tggcgaaggg
     5041 ctcctcgagc acctcaacgg catgttcgcc ttcgccatct gggacctcga ccgcaggaag
     5101 ctcttcctcg cccgcgaccg cctgggcatc aagccgctct actaccaggt gaccggcgag
     5161 gggatcgcct tcgcgtccga ggtcaaggcg ctgctggccg ccgccggccg ccgcccgggc
     5221 gtcgatctcg agatgctcga cgcctacatg agcgtcggct acgtgcctac cgagcggaca
     5281 ctcttcgccg gcgtcaccaa gctccagccg ggctggtgcc tcaccgttca tgacggccgt
     5341 atcgacctgc gccagtactg ggacctgagg ttcgagaccg aggagacccc ggagatcacc
     5401 gaggaggagt gcgtgcggca ggcgaccgac ctgctgcgcg acgcggtgcg gctgcagctc
     5461 aggagcgacg tccccctggg ggtgttcctc tccggcggcg tcgactcctc ggccgtggtg
     5521 gcgctgatgc acgacctggg catccgcgac atccgcacct tcaccgtcgc ctacgatttc
     5581 ggccccggct tcgacgagac ccgctgggcg cggcagatcg cgcagcagtt cggcaccatc
     5641 caccgcgagg tgttcgtcac gccgcaggac ttccaggatt tcatcccgtc gctcgtctgg
     5701 cacatggacg agccggtgac cgagtcggcg gcgatctcgc tctatttcgt ttcgaaactg
     5761 gcgcgcgagg acgtcgtcgt ggtgctctcc ggcgagggct ccgacgaggt cttcggcggc
     5821 tatccgattt ataaatacat gcagacgttg gagcgctacc ggcgcctgcc cggagctctg
     5881 cgccgcggcg tgatcaatcc cgccctgaac ctcttgggcg gcaagtggcc gaagtacacc
     5941 caactcgccg aacaggccct gccggagcgc tatctggggg tctcgttcta tgagacgccg
     6001 gtgaagaatt cgctgctgcg gccggagatc cggagccttt tcaacgggca cgcgatgtcc
     6061 agcgtggcgc ggcgctatta cgaaaagacc gagggcctcg atccgctgct gcgcatgatg
     6121 tatctggacg tcaagagctg gctgccggac gacctgctga tcaaggcgga caagatgacg
     6181 atggcgagct cggtggagct gcgggtgccg ttcctcgatc accgcgtggt ggagctggga
     6241 gcacggatcc cgccgcgcta ccggatcaag gggtggagca cgaagcacat cctcaagaag
     6301 gcgatggagc cctatctgcc gcacgagatc ctgcaccggg gaaagatggg tttcccgacg
     6361 ccgctcgccc gcatgttcca gcgtgagatg aagccctacg tggcggacgt cctcaccagc
     6421 gaccgcttct tcgaccgcgg ctacttccag cccgacgtcg tacgctccat ggtcaccgag
     6481 cacaccgccg gcgagaagga ccatcaccgg gtgctgtggc agctcctggt gctggaggag
     6541 tggcaccggc ggttcgtgga tggggaggag cggggggcgg cgggcgcccc ccgcgggtag
     6601 agagcctaca ccgtctgcgg ctgggccggc gatgctggcg acgccagccg gaccggctcg
     6661 gccgccggcg ccgccatctt cgcctcgccg gccgccctcc ggcccagcac cgccagcatg
     6721 tggcgcgcct cgttatcgag cgtcacgtcc ttgagatcct cgtaaccctc gctccggaag
     6781 agcgccatga tctccggcac ggtgtgccgg tactggtagc gcgggctgta gttgtcgaac
     6841 atttgaaccg cgcgctcgcg ggcggtggcg cggggcggcg ccggctcgcg gcgcagcgcg
     6901 tgcatcaggc gcgacagcgc ggcgtggacc ggcgtcgtcg cgtagcagag ggcatagagc
     6961 acgttcatcg gcatgcgggt ggtcacggcc cgcagcgcga cgttgatcag atggatccag
     7021 gtggggcggc ggcgatagag ctggatgtag agcttgccac ccggccgcac cgctttgctc
     7081 accgaacgga acgcacggta ggtgctcggc gtatggtgca ggaccccgga ggagtggacc
     7141 atgtcgaagc tgcgcggcag gagcggcagc tccagcaggt ttccctgtag atagtgcacg
     7201 cggtgcgaca ccgcgaaacc cttttcgaac aggcgctggc gggccccctc gacgctgcgc
     7261 gagaggtcca tggccacgat ctccggccca tactccgcca ccgagcgggt cagctcgccg
     7321 ttgccgcagc cgccgtcgag gagcgtcgag ctcgccaact cccccggcgt ggtgtcgagg
     7381 ttgtagagga actcctgctt gcgcaggttc gcgtcgtcct tgaaccaggt gtggtcgccc
     7441 tcgcgataga tggcccactg caggccgaag ctgcgcgggc tgcggccgtc tacgacgggc
     7501 gcagcggctt ggggctgcgc tgcggcctgg ggctgcggag cggctgcggg agcaggggcc
     7561 ggagcggcgg ggagggcggc ggcttctccc aggggccggc ccaactggtg ggcgtggcgg
     7621 gcgaagaact ccggatactc ctcgcgggcg ttgcggatca tccgcggcac cccctgcaga
     7681 accgggtaga ccaggccgca ggcgcaggac agcgccccct ccaggatctc ctcccggtag
     7741 caggagttgc aatccggggg cggcgtgatg acccgcggcg aggcgatgcc gtgccgtcca
     7801 cagtaggagg agcaggccgg cggctgcacc ggcgcgggga gctccaccct ctcctcctgg
     7861 aagacctgga gctgcggcct ctcgccgcag cccgggcaga cgatccaatc gagcagacgt
     7921 ttcttcatgg ttcgaaacct ctccgtctct gtgtcggaag ccatttgcga ccggatcaca
     7981 cactgcacac ggatcgcgaa cgacgtccca atgcaacgaa caggccaccg cggtccgtct
     8041 ctccgggaac gcttcttgca ctcagagcag ccggatgttc cggtcatctg ggggcggggc
     8101 agtgtccgtg tccccgaacg caggagaaga gctcatggag atcaagcaac atttctatcg
     8161 ttggctgctc tgcgccctgg tgggcgctgt cgttgccgtc ccggtcgtgg cggatgtggt
     8221 ggtccgcact gaagaggacg ctcccaacgc ctatgcgatc ggcatcggcg acgtcgtcga
     8281 gatctccgtg tggaagaacc cggatctcac cacgacggtg ccggtgcgcc cggacggccg
     8341 catctcggtg ccgctgctgg gcgatctcca ggcggcgggg atgacccctc tcgccctgcg
     8401 gcagacgatc accgaccgtt acaaggagtt cgtcaccgct cccggcgtgt cggtggtcat
     8461 caaggagatc aactcgcgca aggtcttcgt caccggcgag gtggcgacgc cgggggccta
     8521 cgacctgccc ccgcgggcca agctgatgca gctcctggcc atggcgggcg gcctcacccc
     8581 ctatgccaag gggcgcgtgg tggtgctgcg cgaccgcgac ggcgacggca aggaccgccg
     8641 ctacgagatc cagctcgaca ccatcatctc gggccgcaag ccgggcgaca acctggtgct
     8701 ccagcccggc gacaccctcg tcgtcccctg agcgtcgtct cctggcctcc gcttcctgac
     8761 acgctcccct gacgctcttc ctcacggtga cagaatggtg acgcgccggt tccaggccag
     8821 ccggcgcgcc gcttccccac cagggacccc tgcaaccccg ccccccagcg tgtattccag
     8881 gcctcttccc aggcatacct ccctggaaaa agggagaaaa acctacacgg accggggccg
     8941 cggcccctcc tgggcgccgc ccctccgagg ctccgcgcgc aggggggccg cctgcttatc
     9001 gagtcctctc tctggcacct gtcttgcact ttccgggcgg tgaaggtttt gggctggtat
     9061 gacaagagat caaggcacat caggacgccc gccttctggg atcgacacgt cagcgataga
     9121 gaatcgccgg ctgttttagc aatcgttccc ggtcgttgag tcgtggctcg cggacctttg
     9181 tgaggttccg tgatgtctca ggttctttgg ccccggggta tacgcgggga ccatcgccgt
     9241 cgctgacggt ggaggtccct cgcatcgacc agcaagaggt gaacaggcga ggccatggtg
     9301 ggttcctcaa catcgacgaa ggttggcgcg gcgctccggc tgccggctct tttcgctctg
     9361 ctgctggcgg gcatgccggc gggagcgggt gagctgcggc tggtgatcga gggcgaggcc
     9421 ggggtccgca acgacggcaa cctcgagcag cgggcccaga tcgaggcgac cggctccggg
     9481 gacgaggagg acaggaacgt cgcccgtggc ggcctgagct tccagctctc gtacgacctg
     9541 ccgcgcgccg acctggccct gatctacagc ccgtcgtacg aggagagcat cgacgacagc
     9601 gagatctccg gcaccaccca ccgtctggac ctgggctacg agggggagct gacccggcgg
     9661 ctgcggctgc gggtccggga gcgcctgctc aagagcccca acctggacct ctatgccccc
     9721 ctgtccgtgg ccgagccggt cgccgtgacg cgccgcggcg accagctcag ccacggactg
     9781 gacgtcggct tcgagcacga cctcacccgg cgcgccgtgc tggtcgtcgg cgcgcagcac
     9841 agcctgcgca ccttcgacgg cgaggagctg gaggacgccc ccctctatga caccgagacc
     9901 ctggtcggca cgctgggagc caccttctcc ctggccgagg accgccagct cggggtatcg
     9961 gccagtgccg gcgagtacga ctacgagcgc cgcggcgagg ccgatgtgca gaacgtcggc
    10021 gtctcctaca gccaggggtg ggggcggtct ggccgggtgt cggtggaggc cggcctgttc
    10081 tccgtcgagt cgacgctgct ccgccgcggc gtcatcgccc tgccggacgc tccggacgag
    10141 gcgcccggcc ctcccgtcgt cgaggaggtg ctgacggagg aggaggacga ggggtggcgc
    10201 ggcgcggtat cggtctcccg gcagggacgg ttcatcggct ggaacctcgg ctaccggcac
    10261 gatgtgtcgg ccgggttcgg cctgggggag ccgaccgagg cggacagcgc cttcgccggc
    10321 ctctcgtact cgcccacccg gcggctcact ctggggctcg acggcaacgc ctcgcgccac
    10381 gagccgctcg gcgactccga gcgcgccgtc ggcacgctgt tgcagaggca ggacactctg
    10441 aacgagttcg ccgccggcac cgcccggctg tcctggactt ttttccaggg gctgcggctc
    10501 accggcggct actcgcgcat ctggcagagc tccgaggtgg agcccttcga agacctttcc
    10561 tatgaccgtt ttttcctggg tttggcgttc cgggtgtgga gcagcgggga gaccccgcgc
    10621 gagccggacg acccccaagg ggaactgacc gatgaagcac gcgacgatca agaacctgac
    10681 gcccgctgag ctcggcaagt ccgtcctgcg ccgtccaggc tggttcctgg tgccggtgct
    10741 gctgggcctc gtcggcgcct tcctggcgtt gcgcatgctg acgcccatct accgggcgag
    10801 cacgcaggtg ctggtggagc cccagaaagt cccggccgac tacgtcaagc cgacggtgac
    10861 caccgacatc gacgagcggc tcaagacgct cgagcagcag atcaccaacc gctcacacat
    10921 cgagcggatc atccaggaga tgaacctcta ccccgagctg cggcgcgagc tggcgatgga
    10981 gaagctcgtc gaccaggtgc ggcgcaagga cctgacggtg gagcggcgcg gcgacatctt
    11041 cagcatcggc ttcaagagcc aggatccgaa gaaggccgcg gcgacggcga accgcatcgc
    11101 cgagctgttc atccaggaga acctgaagct gcgggagaac caggcgcagg gcacctcttc
    11161 cttcctgagc gccgagctgc agcaggcgaa ggagaagctg gagcagcagg aggcccgggt
    11221 cgccgtgttc aagcagctcc acatgggcca gctcccggag cagcgcgaca ccaacctcca
    11281 gcaggtcggc cagctccagg acaagctgga gatcaacatg gacgccctgg accgggcgga
    11341 gacccgcaag atcctgctgc agagccaggt cgccgaggcg cgccggcagg cgcagatgcc
    11401 ccggcagccc gtgcagccga cgatcctgat gacgactccg ggggcgccgt cgcgcctcga
    11461 ccagctccgc gccgagctca tcgacctgcg ctcgcgcttc accgaccgcc acccggacgt
    11521 gatccggctg cagaaccaga tcgcccagct cgagagggcg gcgagcgagg cgccgccgcc
    11581 cgcgcccccg ccgcagacga tggcgagccg ggagccggag ccggtgcgga tcgaccccga
    11641 gctcctcgcc cagctccaat cggtggacta cgagatccgc aacctgcacg cggagcgggg
    11701 gcgcatcctc gccgacatct cccgggtgca ggcgcggctg gaggagatcc cgcgcgtcga
    11761 gcaggagctg ctgtcgctga cccgcgacta cgagaacaag cagcgcacct atgagtcgct
    11821 gctcgagaag cagagccagg cccgtctcgc agagaacctg gagaagcggc gccagagcga
    11881 gcagttcacc atcctcgaga aggcggtgcc gcccgcggaa cccttcttcc ccaacccgct
    11941 gtacgtgctg gcggcggggc tcgccgccgg cgccctggcc gggatcctgc tctcgctgct
    12001 gcgcgaccag accgactcca cgtacgcgga tggtgacgcg ctccagcagg ccttccccgg
    12061 cgtgcgggtg ctcgccacca tccccatctt cagcgctgag gccgtggctg gatccgccac
    12121 cgcccagagc cgtttcagga ggaactgacc catggatcta ccgctgagga gcgatagggc
    12181 tcatcgggtg gccacgccga cggagaccat ccgcctgcgg gaggacgcgc cggtgatcct
    12241 ccacgtggac ccgccatcct ttccggccga gcagtacagg ctgctggcgg tccaggtcga
    12301 ggaacggctc aaccccatcg gcacctgggg gttcgccctg agcgtcacca gccccgacga
    12361 aggggtgggc aagaccctga ccagcctcaa cctggcgctc accctgacgc gcggcgagga
    12421 gcggcgggtg ctgctgctgg agggggacct ctggcgcccg cgcctccata cctacttcga
    12481 tcccgaggat gtcaaccggc cggggctgca gcaggtgctg gagcgccgga tacagctctc
    12541 cgaggccgtc gtcgccgtcg aggggacgag cctcgacctg ctccccgccg gcgccgcggg
    12601 cttcgccggc gacgtgatgt cgggccgccg catgtccgag gtgctcaccg aggcgcgggc
    12661 ggcctacgag atcgtcgtca tcgactcgcc gccgatgggc atgctggcga gcgcccgctc
    12721 cctcgcctcg cgcgccgacg gcgtggtgct ggtggtgcgc gccggtcaga gcaagcgccg
    12781 gagcatcgag aagaccctgg aagggctcgg gcccgacaag ctgatcggga tggtgctcaa
    12841 cggcatcaag accccgggcc gcggctaccg cggctactac taaggactta ttgggaaaca
    12901 ggaaagagaa gaggccatga tccgcctgtt caatcgttac tggaccatct cggcgttcat
    12961 ttcactggcc gtcgagggtt cgatcctctt cctgtcggtg ccgatggcga cctggatacg
    13021 cctcgggctc ggccctggcg atccgccccc gcgctcgatc caggcgctcg aagggctcgc
    13081 cttcatggtg gtcgggctcc tggcgctcta tatcaacggg ctctacgact tcggtgaacg
    13141 gcagtcgagc cgccacatgt tcatccgcct gctgcgggcg ggatgcctgg ccggcgtcgg
    13201 gctgatggcg gtctacttcc tgttccccaa gttcctgacc ggccgcggca tcttcgccat
    13261 ctcgttcgtg gtggccgcct ggggcgtcac cctctggcgc ctggtgctgc gggcgctgct
    13321 gagcagcgac cgcttcgccg agcgggtgct catcgtcggc tccggcgaga aggcgatcga
    13381 catcgcccgc gagatcctgc agcgcaagca cctgggcttc cgcgtcgtcg gcttcctcga
    13441 caacgatccc aagctgcagg gggtgagcct gatcaatccg cgggtgatcg gcaccaccga
    13501 tcagtgccgc gacctggcga tgctgcacgg cgtgacgcgg gtggtggtgg cggcgaacga
    13561 tttccgtggc ggccaggtaa gcctggacgc cctcctggcg tgcaagacct cggggatcaa
    13621 ggtgcaggag gcctcgagct actacgagca ggtcaccggc aagatcatgc tcgaagggct
    13681 gcgcaagtcc tggctggtct tctccgatgg cttcgtcgtc tcgcgcggcg cgctgttcgc
    13741 caagcgcgtg ctcgacctcg ccgccgccac caccgggctg atcctcgccg cgccggtcat
    13801 gctgctggtg gcgctggccg tccgcctcga ttcgccgggg ccggtcttct tccggcagga
    13861 ccgtgtcggc cgcggcgggc gcgagttcac gctctggaag tttcgctcga tgcggaccga
    13921 cgccgaggcc ggcggcgccc agtgggcggt ccagggggac ccccgggtga cccgtgtcgg
    13981 ccgcttcatc cgcaagaccc ggctcgacga gctgccccag ctctggaacg tgctggtggg
    14041 cgacatgagt ctggtcgggc cgcgcccgga gcggcggatg ttcgtcgacc agctcaagga
    14101 gcagatcccg ctctacgagc agcgcctggt cgtccgcccg gggctcaccg gctgggccca
    14161 gatcaaggcc ccctacgcct cctcgttcga ggagtcgctg gagaagctca agttcgacct
    14221 gtactacatc aagaacctgt ccctgttcct tgacatctcg atccttctgt ccaccgccag
    14281 gacggttctc ctgggaaggg gcgctcgatg accgactccg ccgatcgacc gaagccgccg
    14341 gcgccgctgc tgcggcacgg cacgcgcccg agcgcggccc tgcgcatcct ctatctctgc
    14401 caccgggtgc cctatccgcc ggacaagggg gagcgtatcc gggctttcca tcagatccgg
    14461 gccctgtccc ggcgccaccg ggtgagcctc ttcaccctgg cggacagcgc ggtgccggac
    14521 ctgcggcctc tggaggagat gtgcgagcgg gtggaggtgt tcaacctcaa ccgtcccgcg
    14581 gcctacctgc gctcggccct gtcggtctgg cggcggcgcc cactcaccct gtcgttcttc
    14641 gagtcggcgg agctgaagac acgcctgcag gcgttggcga agagcgagcg gttcgacgtg
    14701 gtggtggtct acagctccgc catggcgccg tacgccgacg cccttcccgg cgtgccggcg
    14761 gtgctggaca tggtcgacgt cgactcggcc aagtgggctc agtattcgtc cttcgcgaag
    14821 cggccgatgc ggccggtgta cgccctggag gcccggcgcc tccaggccta cgaggcgtcg
    14881 ctggtcgatc gcttccagcg cgtggtgctg gtcaccgatg gggaggcgcg gacgctgcgc
    14941 tcgttcgctc cggaggcgcg cccggagtcg atccccaacg gcgtcgactt cgatttcttc
    15001 cgcgccctgg acctgcccaa ggccgagctg ccgacgctgg tcttcaccgg ccagatggac
    15061 tacttcgcca acatcgacgg agtctgccac ttcgcgcgcg aggtcttccc ccggctgcgc
    15121 gcgcgctatc cggacctgca gttcgcggtc gtcggg
//
LOCUS       DASPJI010000551        10191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_602961, whole genome shotgun sequence.
ACCESSION   DASPJI010000551 DASPJI010000000
VERSION     DASPJI010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10191)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10191
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602961"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /gene="mug"
                     /locus_tag="VEL74_15205"
     CDS             <1..531
                     /gene="mug"
                     /locus_tag="VEL74_15205"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HYG63924.1"
                     /translation="TPEEIQAAAGKTLPDVIAPGLRVLFCGINPSLYSAAVGHHFARP
                     GNRFWKTLHASGFTGRVLSPFEDRELLSAGMGVTNVVDRATAAASELESDELEEGGRR
                     LARKVKRYRPAWLAVLGIGAYRTAFGRPKAVLGRQEETVGGAAVWVLPNPSGLNASYQ
                     LDALADAYRELRQATG"
     gene            complement(537..956)
                     /locus_tag="VEL74_15210"
     CDS             complement(537..956)
                     /locus_tag="VEL74_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63925.1"
                     /translation="MKLKTALSLLFATALLALLTAVPASAACTATLNCNNACSVSVVC
                     RNGCEIGCAANGTTASCTGATSCTVGTSSVTCDGTTVSCPSTPRCSSTRTSVYCGFHV
                     IYCPLSCPKSLQQSGAVDETSPLWLRPEAMAAAAQRQ"
     gene            1132..4419
                     /locus_tag="VEL74_15215"
     CDS             1132..4419
                     /locus_tag="VEL74_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016705249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63926.1"
                     /translation="MSTDENAIRFETDPIVFELQGKGWKDLQEAVLLVPKSLGSPGSA
                     PVKADIQPTTTPHGFRFVAAEPSWEESPDEQDEPNRLRVRVVCSGGTEGAEDEAVLTL
                     TWKDVTARVKLLAGVNRVLALSDILREEYRELRPDLAAQLDAAAPATPGDGDGVEVGH
                     DEERVRRLYDVLHKDPKGLAALCFSGGGIRSATFNLGVLQGLASLGILDKFHYLSTVS
                     GGGYIGSWLSSWIHRSGTGAAADHLAAPRQNPEAPEPAPLRHLRQYSNYLTPKLGVFS
                     ADTWTLAAIVIRNLLLNALVILPILAAILTVPLIALARVPRAWKVDADVLFLIAFVCG
                     TLGLFFENLLRASAVPRPGEKAPGWTQAFLRLGLLPLLIGTGFLILAVDRFAPGGTFA
                     FHEVLWRSAVWAIGMPLLAYLAAAALQKPLLGRRQTFLVSDLFALISAGIVITFVYAG
                     ILAHWAEPLVNAHLYLYPILGPGLILGPLLLGKTLFMAFSSPAEEYPGRLASEHGDAD
                     REWWARWSAWILIPTIVWTLGSALVYFAPFLVGETKARISAYLAAGGLGALTSMLGKS
                     AGTHKDSGKASPWRQLALALAAPLFCLVLLVVLSASTQDLLNRFFKASGQTDRQAATS
                     IDPSDQPMIVAAVAPRAEAAVAGETEKKGTWLTSPAEHPPFLGKTWQVFLAVAALLGF
                     GTIMGYFVNVNRFSLQGMYRNRLVRAYLGASNTRRRPNLFTGFDSHDNVRMHTLRAAN
                     RPLHVVNMTLNLVGGKELAWQERKAESFTATPLHCGAASLGYRRSQIYGGQHGISLGT
                     AMATSGAAANPNMGYYSSPPVTFIMTLFNARLGIWLGNPGKLGRLTATRSGPRFSSRV
                     IFAEALGFTDSSHPYVNLSDGGHFENLGLYEMVRRRCRFIVVCDAGGDPDCAFDGLGN
                     AIRKVRIDLGIPIDFDDRIHIYPKAKPGQTPPADARYCAIGTIHYDQVDGAGAPEGTV
                     IYIKPAICGTEPYDVTNYANASATFPHETTVDQWFSETQFESYRALGKSAVVRMGSFA
                     AVSDMATFKQSVEKYIARAQQGTPKVEVILPSILGGLAAAAAPPLAPATPDALKEGNG
                     GTQTQRA"
     gene            complement(4421..6184)
                     /locus_tag="VEL74_15220"
     CDS             complement(4421..6184)
                     /locus_tag="VEL74_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYG63927.1"
                     /translation="MNLTPELVADILVRQGYITSEQGDAIRQESKLLPGRLRNTRAFE
                     QKAIAYDLILEMRLPNRRDSGAPLGEIEIAQAIAADARLGHVRIDALNLNADLIESKM
                     SRPFARRHRMIPLEMVNGRLKVACANPYDLEGIDSFKRIAGRDIDLVIASEPDVLKAI
                     TEFYGLRHSVKRAEKDLTAGIDLGNLEQLVRMKSENEIESSDQHVVNAVEFMLQHAFE
                     SRASDIHIEPKRGESLIRFRIDGVLHDIQRMPKIVHAAVTSRIKTMSRLDIAEKRRPQ
                     DGRVKTTRAGRDVELRVSTLPVAFGEKVVMRIFDPQVFMQDLTGLGLYADEMQHFNDF
                     ISRPHGIFLVTGPTGSGKTTTLYSALKIIATREVNVTTIEDPIEMVYEDFNQTAVQEK
                     VGITFSGALRHILRQDPDVIMVGEIRDPETAQYAIQAALTGHLVFSTLHTNDAPTSIS
                     RLSDLGIERFMISSTLVGAMAQRLVRRICPNCVTERFLTPDEVTTLRLAVPQGKRVKV
                     KEGEGCFECRGTGYLGRTGIFEILPMDDAIKNLVVKGADAPEIKREATKNGMRTLRQS
                     ALRKLAEGITTFEEVVRVTGI"
     gene            6267..7193
                     /locus_tag="VEL74_15225"
     CDS             6267..7193
                     /locus_tag="VEL74_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYG63928.1"
                     /translation="MYDVIVIGGSWAGLAAALQLVRARRRVLIVDAGRPRNRFARTAH
                     GFLGQDGKTPVAIVEEVRGQVLSYPTAEHRVDEALQAWRAGEGSGEGFAVGLASGATV
                     HGRRLMLATGIVDDLPEIPGLRERWGATVLHCPYCHGYEMADRRLGVLAVGEHSLHSA
                     MLIRDWSEHVTLFTDGVLEPGEGQRAALEARGVRVEERTVEELLGSGPALEGVRLRGG
                     DVVPLEALFTTSRTRMASPLAEQLGCEFKDGRFGPVIVTDRLRETTVPGVYAAGDAVR
                     SSHNATWAASDGVTAGISAHQSLLPRLPASPE"
     gene            7234..8436
                     /locus_tag="VEL74_15230"
     CDS             7234..8436
                     /locus_tag="VEL74_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HYG63929.1"
                     /translation="MRKFAPLLLLLACAPGGDDRAGASAPEASPAQDTQSTSSQAGAA
                     QPARRASRLPLDRIKLPAGFSIDVFSEDVPGARSLARSPKGIIYVGTQREGKVYALVD
                     REGDGRADQVHTIARGLTQPNGVAWRDGSLYVAEISRILRFDGIDERLSDPPAPKVVT
                     ADYPREGHHGWKFIRFGPDGWLYVPVGAPCNICERENPIYSSITRIKPDGTGREVFAS
                     GVRNTVGFDWHPQTKELWFTENGRDWLGDNRPPDELNRAPRAGLHFGFPYCHGKDIKD
                     PEYGDKRACSETVPPARELDPHVAALGTRFYTGTMFPAEYRNQIFFAEHGSWNRSDPI
                     GYRVMLVRLQGEKAVSYEPFAQGWLSGGKVWGRPVDVEVMPDGALLVSDDHAGAIYRI
                     THRGGGAR"
     gene            8423..9259
                     /locus_tag="VEL74_15235"
     CDS             8423..9259
                     /locus_tag="VEL74_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63930.1"
                     /translation="MALADRLRDRARAVRDWFFPPEVPVPEGVRRLVAAVYPTLDLRR
                     VSFHRGVPHLLGLVDSHAMAVPAALSPRRIRIYVRPRYWDTATVEGLGLLLHEAFHAL
                     QIQETGPGLGLLRPFTILYLACAGGSRFLYHLHPMEVDAYRVAGRRRSLFEQAFLAQP
                     GSLVPVLVADGPVTPETVPCLPEGGPVIPSSEPSFWGRLAASTPVARGIARRARRFQG
                     IPALLLAAAAWPLIALWLLAWTVVVALLWLARLVVEAAGALAAALLWTLAVLVRPFRR
                     LI"
     gene            9317..>10191
                     /locus_tag="VEL74_15240"
     CDS             9317..>10191
                     /locus_tag="VEL74_15240"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HYG63931.1"
                     /translation="MLKRFLVPGLAFVLFLGWSGVGRADVVIDWNNVTLDAIRVDKTS
                     PPKAARALALVQVSVFDAITSVLGPYATHHENPQQFPGPSTLMAATAAGAAAAHTVLV
                     ELFPSRKETFDAALAASLAAVTDPVAKNTGESWGRLVAGQILNSRENDGSAATMAYEA
                     PTGANWWAPTPPAFAAPLLPNWPSVTLWSGLNATQFRQGPPPPSTSAEYAAAFREVRR
                     LGRVDSQFRTPEQTQIALFWADGPGTATPPGHWFVIAQDVSQQQGLSLVENARLFALL
                     GIAVADAAVVSWDHK"
BASE COUNT         1490 a         3489 c         3540 g         1672 t
ORIGIN      
        1 accccggagg agatccaggc cgccgccggc aagaccctgc cggacgtcat cgcgccgggg
       61 ctgcgggtgc tgttctgcgg gatcaacccg agcctctact cggcggcggt ggggcaccac
      121 ttcgcccggc cgggcaatcg cttctggaaa accttgcatg cctccggctt caccggccgt
      181 gtcctctcac ccttcgagga ccgcgagctc ttgagcgccg gcatgggcgt caccaatgtg
      241 gtggaccggg cgacagccgc ggcatcggag ctggaaagcg acgagttgga ggagggcggc
      301 cggcggctgg cgagaaaggt gaagcgctac cgccccgcat ggctcgccgt gctgggcatc
      361 ggcgcctacc gcacggcctt cggcagaccg aaggccgtgc tcgggcggca ggaggagacg
      421 gtagggggcg ccgcggtctg ggtgctgccg aatcccagcg gattgaacgc cagctatcag
      481 ctcgacgccc tggcggacgc ctaccgcgag ctgcggcagg cgaccgggta gctcctttac
      541 tggcgctgcg ccgccgctgc catcgcttca ggccgaagcc agaggggcga cgtctcatcc
      601 accgcgccac tttgctgcag gctcttgggg caggagagag ggcagtagat gacgtggaag
      661 ccgcagtaga ccgaggtacg agtggatgag caccgcggag ttgaggggca gctcacggtc
      721 gtgccgtcac aggtgacgga gctcgtcccg accgtacaac tggtcgcgcc ggtacagctc
      781 gcggtagtgc cgttggcggc acaaccgatc tcgcaaccgt tccggcagac cacgctcacg
      841 gaacaggcat tgttgcagtt gagcgtcgcc gtgcacgccg ccgaggctgg aacggcggtc
      901 agcaatgcca ggagagcggt cgcgaagagg agggacaacg ccgtcttcag cttcatgatc
      961 tcagctcctt ttcgagtgga catgcggata ttgctgtcga tgcattctcg cggagtgtcg
     1021 aatctgctgt caaggtggat ggctggaccg cctgcaggtc tgttaagatc caacgacaaa
     1081 tcagacagta caatctgggc cagccctgcg ccggctcgtg gagggatctc gatgagcacc
     1141 gacgagaacg ccatcaggtt cgagacggat cccatagtct tcgagctgca ggggaagggc
     1201 tggaaggatc tgcaggaggc cgtgctgctc gtgcccaagt cgctggggag cccgggctcc
     1261 gcgccggtca aggcggacat ccagccgacc acgacgcccc acggcttccg gttcgtcgcg
     1321 gccgagccgt cgtgggagga gagcccggac gagcaggacg agcccaaccg cctgcgggtc
     1381 cgggtggtct gcagcggcgg cacggagggg gccgaagacg aggccgtcct gacgctgacg
     1441 tggaaggatg tcacggcgcg ggtcaagctc ctggcaggag tcaaccgtgt cctggccctc
     1501 tcggacatcc tgcgcgagga gtaccgcgag ctgcggccgg acctggcggc acagctcgac
     1561 gcggcggctc cggcgacccc aggggacggc gacggcgtcg aggtgggcca cgacgaggag
     1621 cgggtccgcc ggctctatga cgtcctccac aaagatccga agggactcgc ggccctctgc
     1681 ttctcgggcg gcggcatccg tagcgccacg ttcaatctcg gcgtcctcca gggcctggcc
     1741 tccctcggca tcctggacaa gttccactat ctctcgacgg tctccggcgg cggctacatc
     1801 gggagctggc tgagcagttg gattcatcgc tccggcaccg gcgccgcggc cgaccatctc
     1861 gcagcgcccc ggcagaaccc ggaggcaccc gagccggcgc cccttcgcca cctgcgccag
     1921 tacagcaact atctcactcc gaagctgggc gtcttctcgg ccgatacctg gaccctggcc
     1981 gcgatcgtga tccgcaacct gctgctcaac gcgctggtga tccttcccat cctggcggcg
     2041 atcctgaccg tgccgctgat cgccctggca cgggtgccca gggcctggaa ggtggacgcg
     2101 gacgtgctct tcctgatcgc tttcgtctgc ggcaccctcg gcctgttctt cgagaacctg
     2161 ctgcgcgcca gcgccgtgcc gcggcccgga gaaaaagcgc ccggttggac ccaggcgttc
     2221 ctgcgattag ggctcctgcc gctgctgatc ggtacgggct tcctgatcct ggcagtggat
     2281 cgcttcgccc ccggcggcac cttcgccttc catgaagtgc tctggcggtc cgcggtctgg
     2341 gcgatcggca tgcccctcct ggcctacctg gccgcggcgg cgttgcagaa acctctcctt
     2401 ggccggcggc agaccttcct cgtctccgat ctcttcgcgt tgatcagcgc cgggatcgtg
     2461 atcaccttcg tctacgccgg catcctcgcc cactgggccg agccgctcgt caacgcgcat
     2521 ctgtatctct acccgatcct cgggcccggc ctgatcctcg gcccgctgct cctgggaaaa
     2581 acccttttca tggccttctc gagtccggcc gaggagtacc ccgggcggct ggcttcggag
     2641 cacggtgacg ccgaccggga gtggtgggcg cgctggtcgg cctggatact gatccccacg
     2701 atcgtctgga ccctcggcag cgctctggtc tacttcgcac cgttcctggt gggggagacc
     2761 aaggcccgga tctccgccta cctcgccgcc ggcgggctcg gcgcgctcac cagcatgctc
     2821 ggcaagagcg ccgggaccca caaggacagc ggcaaggcga gcccgtggcg ccagttggcg
     2881 ctggccctgg cggcgccgct gttctgcctc gtgctcctgg tggtgctctc cgccagcacg
     2941 caggacctcc tgaaccggtt cttcaaggcg agtgggcaga cggaccggca ggccgctacg
     3001 tccatcgatc cgtccgacca gccgatgatc gtcgcggccg tggctcctcg ggccgaagcg
     3061 gcggtggccg gggagaccga gaagaaaggc acttggctga cctcgcctgc cgagcacccc
     3121 cctttcctgg gcaagacctg gcaagtcttc ctggcggtgg cggccctctt gggttttggc
     3181 acgatcatgg gctacttcgt caacgtcaac cgtttctctc tccaggggat gtaccgcaac
     3241 cggctggtgc gcgcctacct gggagcgagc aacacgaggc ggcggcccaa cctgttcacc
     3301 ggtttcgatt cgcacgacaa cgtgcgcatg cacactctac gagccgccaa ccggccgctc
     3361 cacgtggtca acatgacgtt gaacctggta gggggcaagg agctggcttg gcaggagcgc
     3421 aaggcggaga gcttcaccgc gaccccgctc cactgcggcg ccgcgagcct cggctatcgc
     3481 cgttcccaga tctacggcgg ccagcacggc atctcgctcg gcaccgcgat ggcgacctcg
     3541 ggcgccgcgg cgaaccccaa catgggctac tactcctcgc cgccggtcac gttcatcatg
     3601 accctgttca acgcccgcct ggggatctgg ctcggcaacc ccggcaagct cggacgcctg
     3661 accgccaccc gcagcgggcc gcgtttctcg agccgcgtca tcttcgcgga ggcgctgggc
     3721 tttaccgaca gctcccatcc gtatgtaaac ctttcggacg gcggccattt cgaaaacctc
     3781 gggctctacg agatggtgcg gcggcgctgc cgtttcatcg tggtctgcga cgccggcggc
     3841 gatcccgatt gcgccttcga cggcctgggc aacgccatcc gcaaggtccg gatcgacctc
     3901 gggataccga tcgatttcga cgaccgcatc catatctatc ccaaggcgaa gcccggacag
     3961 acgccgccgg ccgatgcccg ctactgtgcc atcggcacga tccactacga tcaggtggac
     4021 ggcgcgggcg cgccggaggg cacggtgatc tatatcaagc cggcgatctg cggcaccgag
     4081 ccgtacgacg tcaccaacta cgccaacgcc tcggccacct tcccgcacga gacgacggtg
     4141 gatcagtggt tcagcgagac gcagttcgag agctaccggg cgctgggcaa gtcggcggtg
     4201 gtgaggatgg ggagcttcgc cgcggtgtcc gacatggcca cgttcaagca gagtgtggag
     4261 aagtacatcg cccgggctca gcaggggacg ccgaaggtgg aggtgatcct gccgagcatc
     4321 ctgggggggc tcgcggcggc cgccgcgccg ccgctcgcgc ccgccacgcc ggacgctctg
     4381 aaggaaggca acggggggac ccagacccag agggcttgaa tcagatcccc gtcacccgca
     4441 ccacctcctc gaaggtggtg atcccttccg cgagcttgcg gagggcggac tggcggaggg
     4501 tgcgcatgcc gttcttcgtg gcttcgcgct tgatctcggg ggcgtcggct cccttgacga
     4561 ccaggttctt gatcgcgtcg tccatcggca ggatctcaaa aatgccggta cggccgagat
     4621 agcccgtgcc ccggcactcg aagcagcctt cgccctcctt caccttgacc cgtttgccct
     4681 gcggcaccgc cagtcgcagc gtcgtcacct cgtcgggggt gaggaagcgc tcggtgacgc
     4741 agttggggca gatcctgcgc accagccgct gcgccatggc tcccaccaga gtcgagctga
     4801 tcatgaagcg ctcgatcccc aggtcggaca ggcggctgat cgaggtgggg gcgtcgttgg
     4861 tgtgcagggt ggagaagacc aggtggccgg tgagggcggc ctggatggcg tactgggccg
     4921 tctccgggtc ccggatctcg cccaccatga tgacgtccgg gtcctgccgc aggatgtggc
     4981 gcagcgcgcc ggagaaggtg atccccacct tctcctgcac cgccgtctgg ttgaagtcct
     5041 cgtagaccat ctcgatgggg tcctcgatgg tggtcacgtt gacctcgcgc gtggcgatga
     5101 tcttgagcgc cgaatagaga gtggtcgtct tgcccgagcc ggtggggccg gtgaccagga
     5161 agatgccgtg cgggcggctg atgaagtcgt tgaagtgctg catctcgtcc gcgtagagcc
     5221 ccaggccggt caaatcctgc atgaagacct gaggatcgaa gatgcgcatg accacctttt
     5281 cgccgaaagc caccggcagg gtggagacgc ggagctctac gtcgcggccg gcgcgggtgg
     5341 tcttgacgcg gccgtcctgc gggcggcgct tctcggcgat gtccagacgc gacatggtct
     5401 tgatgcgaga ggtgaccgcg gcatggacga tcttgggcat ccgctggatg tcgtggagca
     5461 cgccgtcgat gcggaagcgg atcaggctct cgccgcgctt cggctcgatg tggatgtcgc
     5521 tcgcgcggct ctcgaacgcg tgctggagca tgaactccac ggcgttgacc acgtgctggt
     5581 ccgaggactc gatctcgttc tcggatttca tccgcacgag ctgctcgagg ttccccaggt
     5641 cgatgcccgc ggtcaggtcc ttctcggccc gcttgaccga gtggcgcagg ccgtagaact
     5701 cggtgatcgc cttgaggacg tccggctccg aggcgatgac caggtcgatg tcacggccgg
     5761 cgatgcgctt gaaggagtcg atgccctcga ggtcgtaggg gttggcgcag gccaccttca
     5821 accggccgtt gaccatctcg agagggatca tccggtggcg ccgcgcgaac ggccgcgaca
     5881 tcttggactc gatgaggtcg gcgttcaggt tgagggcgtc gatccgcacg tggccgagcc
     5941 gggcgtcggc ggcgatggcc tgggcgatct cgatctcccc cagcggcgcc ccggagtcgc
     6001 ggcggttggg gagccgcatt tccaggatga ggtcgtaggc gatggctttc tgctcgaaag
     6061 cccgggtgtt gcgcaggcgc cccggcagga gcttggactc ctggcggatg gcgtccccct
     6121 gctccgaggt gatgtacccc tggcgcacca ggatgtcggc gacgagctcc ggagtgagat
     6181 tcatgtgagg ggaagtatat gatggacgcc aggaacggcc gatgcctgac tctccctctt
     6241 ccttttcgca cgagcggctt cctctcatgt atgacgtcat cgtgatcggt ggcagttggg
     6301 cgggcctcgc cgccgctctg caactcgtcc gtgcgcggcg ccgggtgctc atcgtggacg
     6361 ccgggcgccc gcgcaaccgc ttcgcgcgga cggcccacgg cttcctcggt caggacggca
     6421 agaccccggt cgcgatcgtc gaggaggtgc gggggcaggt gctgtcctat cccacggccg
     6481 agcaccgggt ggacgaggcg ctccaggcgt ggcgcgctgg cgagggctcg ggcgagggct
     6541 tcgcggtcgg gctcgcctcg ggggccaccg ttcacgggcg tcggctgatg ctggcgaccg
     6601 gtatcgtgga cgacctgccg gagatccctg ggctgcgcga gcgctgggga gcgacggtgc
     6661 tgcactgccc ctattgccat ggctacgaga tggcggaccg ccgcctgggc gtgctcgcgg
     6721 tgggggagca ttccctgcac agcgcgatgc tcatccggga ttggagcgag cacgtgacgc
     6781 tgttcaccga tggcgtcctg gagcccggcg agggccagcg cgccgccctg gaggcgcgcg
     6841 gcgtgcgcgt cgaagagcgg acggtggagg agctcctcgg ctccgggcct gccctggagg
     6901 gcgtccggct gcgcggcggc gacgtcgtcc cgctcgaggc gctgttcacc acctcccgca
     6961 cccgcatggc gagcccgctg gccgagcagc tcggctgcga attcaaggac ggccgcttcg
     7021 gcccggtcat cgtgaccgac cggctccggg agacgacggt gcccggcgtc tacgcggccg
     7081 gagacgcggt gcgctcgtcg cacaacgcca cctgggcggc ctcggacggg gtgactgccg
     7141 gcatctccgc ccatcagtcc ctgttgccgc gccttccggc ttcgccggag tagggggccc
     7201 ggcgccgccc ggcgtatggg gtagactgct cccatgcgca aattcgcccc actcctactg
     7261 ctcctcgcct gcgccccggg gggcgacgac cgcgccgggg cctcggctcc ggaggcgtcc
     7321 cccgcccagg acacccagag caccagctcc caggccgggg cggcccagcc cgcccggcgc
     7381 gcctcccggc tgccgctcga ccgcatcaag ctcccggccg gcttctcgat cgacgtcttc
     7441 tccgaggacg tgcccggcgc ccggtcgctc gcccgctccc ccaaggggat catctatgtg
     7501 ggcacccaga gagagggcaa ggtctacgcg ctggtggacc gcgaggggga cggccgggcc
     7561 gaccaggtcc acaccatcgc ccgcgggctg acacagccca acggcgtggc ctggagggac
     7621 ggctccctgt acgtggccga gatctcccgc atcctgcgct tcgacggcat cgacgagcgg
     7681 ctgagtgatc cccccgcgcc caaggtggtg accgccgact acccccgcga ggggcaccac
     7741 ggctggaagt tcatccgctt cggcccggat ggctggctct atgtgccggt gggggcgccc
     7801 tgcaatatct gcgagaggga gaaccccatc tactcgtcga tcacccggat caagccggac
     7861 ggcaccggcc gggaggtctt cgccagcggt gtccggaata ccgtcggctt cgactggcac
     7921 ccgcagacga aggagctgtg gttcaccgag aatggccgcg actggctggg ggacaaccgg
     7981 ccgccggacg agctgaaccg cgccccccgc gccggtctcc acttcggctt tccctactgc
     8041 cacggcaagg acatcaagga ccccgagtac ggcgacaagc gcgcctgctc ggagaccgtg
     8101 ccgccggcgc gggagctgga ccctcacgtc gccgccctcg gcacgcgctt ctacaccggg
     8161 accatgttcc ccgccgagta ccggaaccag atcttcttcg ccgagcacgg ctcgtggaac
     8221 cggagcgatc ccatcggcta ccgggtgatg ctggtccggc tgcaagggga gaaggcggtc
     8281 tcctacgagc ccttcgccca ggggtggctg agcggcggca aggtgtgggg ccggccggtg
     8341 gacgtggagg tcatgccgga cggggcgctc ctcgtctccg acgatcacgc gggcgcgatc
     8401 tatcgcatca cccaccgagg cggtggcgct cgctgaccgg ctccgggacc gcgcccgcgc
     8461 cgtccgggac tggttcttcc ctcccgaggt gccggtcccg gagggggtgc ggcggctcgt
     8521 ggccgcggtc tatcccaccc tcgacctgag gagggtgagc ttccaccggg gcgtgccgca
     8581 cctcctcggc ctggtggaca gccacgccat ggcggtgccg gcggccctct ccccccgccg
     8641 tatccggatc tacgtccgcc cccgctactg ggacacggca acggtggagg ggctgggcct
     8701 cctgctccac gaggccttcc acgcgctgca gatccaggag acggggcccg ggctcggctt
     8761 gctccggccg ttcaccatcc tctacctggc gtgcgccggg ggctcgcgct tcctctatca
     8821 cctccacccc atggaggtgg acgcctaccg ggtggcgggc cggcggcgca gcctgttcga
     8881 gcaggccttc ctggcccagc cgggatcgtt ggtgccggtg ctggtggcgg acggaccggt
     8941 gacgccggag accgtgccat gcctgcccga gggggggccg gtgatcccgt ccagcgagcc
     9001 gagcttctgg ggccggctgg cggcaagcac tcccgtcgcc cgggggatcg cccgccgggc
     9061 gcgccgcttt caaggcatcc ccgcgctgct gctcgcggcg gcggcctggc cgctgatcgc
     9121 cctctggctc ctggcctgga ccgtggtggt ggccctcctc tggctggcac gcctggtcgt
     9181 ggaggccgcg ggcgccctgg ctgctgcgct gctctggacc ctggccgttc tcgtccgccc
     9241 ctttagacgc ctgatctgag gtatcgttgg cctcactgac tgctttgttc tgaggagaac
     9301 ccaaaggagg ggttgcatgt tgaaaaggtt tctcgtcccc gggcttgcct tcgtcctgtt
     9361 cctgggatgg agcggggtgg gccgggccga cgtggtcatc gattggaaca atgtgacgct
     9421 cgacgccatc cgggtggaca agacgtctcc gccgaaggcg gcacgtgccc tggccctggt
     9481 ccaggtatcg gtcttcgacg cgatcaccag cgtcctgggg ccgtacgcga cgcatcatga
     9541 gaacccgcag cagttcccgg ggccgtccac gctcatggcg gccacggcgg ccggggcggc
     9601 cgcggcgcat acggtgctcg tggagctgtt cccgtcgcgg aaggagacgt tcgacgcggc
     9661 cctggccgcc tccctggcgg cggtcacgga ccccgtggcg aagaacacgg gcgagagttg
     9721 ggggcgtctg gtggccgggc agatcttgaa cagccgtgag aacgacggct cggccgcgac
     9781 gatggcctac gaggcaccga ccggcgccaa ctggtgggcg ccgacgccgc cggccttcgc
     9841 cgcgccgctg ctgccgaact ggccctcggt cacgctctgg agcggcctga acgccacgca
     9901 gttccggcag ggtccgccgc cgccctcgac cagcgccgag tacgccgcgg cgttccgcga
     9961 ggtccgccgg ctgggccgcg tggatagcca gttccgcact ccggagcaga cccagatcgc
    10021 cctgttctgg gccgacggcc ccggcaccgc gacaccgccc ggacactggt tcgtcatcgc
    10081 ccaggatgtc tctcaacagc aggggctgag cctggtggag aacgcccggc tgttcgcgct
    10141 cctgggtatc gccgtggccg acgcggccgt cgtctcctgg gaccacaagt a
//
LOCUS       DASPJI010000552        17567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_205840, whole genome shotgun sequence.
ACCESSION   DASPJI010000552 DASPJI010000000
VERSION     DASPJI010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17567)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17567
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205840"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VEL74_15245"
     CDS             <1..242
                     /locus_tag="VEL74_15245"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYG63932.1"
                     /translation="LGVLISFLLRRRDMLTPGAKSLLGQLVLWAGINVVYGFINPGID
                     NSAHLGGFAAGLLLGLILRERRRLRAPAAYPEIEA"
     gene            314..2884
                     /locus_tag="VEL74_15250"
     CDS             314..2884
                     /locus_tag="VEL74_15250"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYG63933.1"
                     /translation="MKLSLLQRLAMFARHRYRTVFAAFVVLVIVSAALISRLSFDTDM
                     LSLLPKEDPAVRTYVETLEDFGSSTYLLVAIRIPEGAVVDPYETLADDLAGRLAQLPE
                     LKSVQHKIGEPQELLATFYPKALLFLDDAGRRQLEARLSDTGIERRVSELRRQLKTPQ
                     GMAAKELLRLDPLGMSDVFLSRLQSSRGSLRVDWTSGYYLSRDHRLLLILAEPVRPPQ
                     DIDFVERLAVQVDRAVDEARGEWEEIAGPGVKAPEVVVGGPHLTALGDAALIKKDMIL
                     NLGTAVPSVLLFFWFTFRRPSSLMYALLPMVTGLALTFAFAELTIGSVSSATSVVAAL
                     LAGLGIDFVIVSYGRYIEERQKGATLEAALLAMSGSTGRAVLVGAVTTTVTFWAFLFT
                     RFTGLRQMGLLTGTGILFCVLSVFLLLPALLAWGEDRHQRRRTQPKLYLHSFGSEGLM
                     RLSLRHPWPVCLAGIAITLAALALAVNIEFDESMKTMRPKGNRGIDVAAEVGKEFGSG
                     FDSMTVVLSGKTPEEVIELSGRAAHGAQRMVDTGTLYGFSGVTSLIPSPSQQREALAW
                     IEREKGDTLDLERIRATFARATAAEGLRAEGFTEGFDLLASAVSLREPIGPESFQESP
                     QTRLLLERYLVKTDRGWKAAVYLYPLDNRWRREPPPDALHLVDKLGPQAALSGTNVVN
                     QRVRSLVLRDAWVAGIIGVVLVALLIWMDFRSLPRTLASLVPLTVGLIWMVGAMVLVN
                     TPMNFINIFVTTMIIGIGVDYGVHILHRFQEVQDYPRREFENAVVETGKAVVAASVST
                     IFGFGSIMFSHYPGLVSTGKVAVLGAVTTCLVSITLVPAVLVWRYERRRKRVPVGEAA
                     "
     gene            3275..3634
                     /locus_tag="VEL74_15255"
     CDS             3275..3634
                     /locus_tag="VEL74_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63934.1"
                     /translation="MPSHGDRTPNGVDLSRPTESTRRHLYELVVHVYFHPTPEEVEKA
                     QSDPGNHWVPDYGPDEARLHVFYAYGRWFASWEALEEKKGAAADPSLPEWRRWSVVRI
                     VEDADPSSATGVTFLEV"
     gene            complement(4209..7094)
                     /locus_tag="VEL74_15260"
     CDS             complement(4209..7094)
                     /locus_tag="VEL74_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYG63935.1"
                     /translation="MKRNLWLVLVLLMVPALVLAQAQTTGRINGRVTDENGAPLPGAT
                     VTVNNQSLQLERTATTGQNGDFLFALLPTGNYEVTVASEGRQPEVYRFRLELGQTVPV
                     NAQLAPGQVVSEEITVTGVATALETTTIGENFDYETEVEALPITARDIESVATLSPNI
                     SFGPTGGTLSISGAPSFDTTVMLDGAEVSDPYFGSAPDLYLEDAVEEVQVLTSGVSAR
                     YGRFQGGIINAVTKSGSNEFEGTLRAELENEDWNSSSPFNEVREDDLQETYQATLGGY
                     IVKDRLWFFVGGRTIPESQTSRTASGTGEAITNTYNEDRWQAKLRGALSENHLLDLSY
                     LSFDSESLNQAGLTPGEARAVGTRTDPRETITASYQGVLGSNTFLEFQATQKNVEINS
                     GGDPAKGDPFIEYFTNTVYNNGWWDFTDPSIRDNETAALNLTHSLSTESLGSHTLEGG
                     VQFVRSTTGGENRQSSTGYNLLALNTGRFYAGQVGGTPRYNFETGYMYRWLAVPLGGD
                     QQLDNLGVYLQDGWTLNNWRFDIGVRYDQYDGSGPLAQFNLSFDDIAPRLGVTYSITP
                     SWQVQATYGRYVSRFNDNVANNATGVANAPRIEHLYTGPDLSGLTADQVQQILRDDQF
                     WTIVTSYIDPTQPTTFLADDINAPYADDYNLSIRHALPRNSGSVVLSYIRRDYEDMLD
                     DFIGLVCTNQGLCDAGDTTSVVAPGGGAAFPLDTTIWANNPDAVREYRALTAVFDYRP
                     TSRWSLSGNYTYSTTEGNYEGEAGNQPSSGSNLGNYPLSVINSSPYGYTDDDIRHRVN
                     LLGTYSFDFNRAGALTLGSIVYYQSGRNWSRTASRPYTATPGYISGGNPPGTFTRFFG
                     ERGSERFNDWWQLDLSARYELPIFSDFNVFLKLAVTNVLDNDELRAFQTTGTTVLNAD
                     GTRSWAPAGTCGLDDEPNRNCSSFGRIRNELDYQPPRAYLVTVAFDF"
     gene            7480..7556
                     /locus_tag="VEL74_15265"
     tRNA            7480..7556
                     /locus_tag="VEL74_15265"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7514..7516,aa:Val,seq:gac)
     gene            7633..7709
                     /locus_tag="VEL74_15270"
     tRNA            7633..7709
                     /locus_tag="VEL74_15270"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7667..7669,aa:Val,seq:cac)
     gene            8005..8613
                     /gene="infC"
                     /locus_tag="VEL74_15275"
     CDS             8005..8613
                     /gene="infC"
                     /locus_tag="VEL74_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003944008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HYG63936.1"
                     /translation="MNERIRVREVRLVESDGSQAGIVTIEDALQRARTQELDLVEVAP
                     TAKPPVCRIMDFGKFLYQQKKKAHESKKKQKVIHIKEVKFRPNIDDHDYDFKLKNVVR
                     FLGEGDKVKATIQFRGREMARQDLGHKLVKRLQADLGESGVLESHPEMTGNRMHVIFG
                     PPRNQVQVAAAPKRAAPAGERAERPERSDARAENPEPRPASS"
     gene            8721..8915
                     /gene="rpmI"
                     /locus_tag="VEL74_15280"
     CDS             8721..8915
                     /gene="rpmI"
                     /locus_tag="VEL74_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HYG63937.1"
                     /translation="MPKQKTHKGAAKRFKLTATGKVKRHQSMHSHILTKKTTKRKRNL
                     RKATMVSPAFEGIIKDLLHS"
     gene            9087..9473
                     /gene="rplT"
                     /locus_tag="VEL74_15285"
     CDS             9087..9473
                     /gene="rplT"
                     /locus_tag="VEL74_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HYG63938.1"
                     /translation="MARIKRGNKRVQRRKKILSLASGFWGMKSKSHRMAKQAVDKALN
                     YAYRDRRVRKREMRSLWIVRINAAARLHGLSYSRLIAGLKAAGSDLDRKVLADIAVRD
                     AEAFGGIAEMAKKALGNKQQSKPAEA"
     gene            9592..10563
                     /gene="pheS"
                     /locus_tag="VEL74_15290"
     CDS             9592..10563
                     /gene="pheS"
                     /locus_tag="VEL74_15290"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HYG63939.1"
                     /translation="MADRQAWEELRLRWVGRKQGIVRSLLDDIKKVEPAGRRAYGEAV
                     NTLKETVEARLAALDEEIGRREREAALRRSTVDVTLPGRRPAIGSIHPITLVMWEMEE
                     IFASLGYSVAEGPEVEDDYHNFGALNFPDDHPARDTQDTLFLDNGLLLRTHTSPVQIR
                     TLLSRKPPIRVICPGRVFRNDNDLRHSPMFHQVEGLAVAEGITVGHLKGTLQAFLQRL
                     FSPETQIRLRPSFFPFTEPSAEVDVTCANCKGKGCEVCSGTGWMEILGCGMVDPRVLA
                     GCGIDPDVYSGFAFGMGVDRVAMNRYGIPNIRMLFDNDERLLRQVRE"
     gene            10560..12614
                     /gene="pheT"
                     /locus_tag="VEL74_15295"
     CDS             10560..12614
                     /gene="pheT"
                     /locus_tag="VEL74_15295"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HYG63940.1"
                     /translation="MKFSLEWLSDYVELPAEPRELATRLTAAGLAVEDVEDGEHGTLF
                     DIDVTTNRPDCMNHFGLAREISVLLEKPLRRPAADPAEATERAADAARVVIEDSEGCP
                     RYAARVIRGVKVGPSPDWLRRRLQAVGLRSINNVVDVTNYVLWELGQPLHAFDLAKLA
                     GATVVVRRARAGERLVTLDGAERELDPEMLVIADAERAVALAGVMGGADSEVTDGTTD
                     ILLESAHFGRRRVRQAARRLGIHSDASHRFERGADPEICAEAASRAAKLIAEVAGGEV
                     LAGVIDVRAAEVPARRGRLDLDRLDAFAGAAVPAADAERWLTGLGFRLEPDGERAWRV
                     TVPSWRTYDFEPRPSGEIYPADLYEEVLRVFGLDRIPAALPGIPGPDAPRAENQVRRT
                     RVRRYLAAAGFAEAVHFAFHDPAADAAYPSLRPDTKPLPLANPLSERYSVMRRSLIPN
                     LVESARFNQRRGRESVRLFEVATVFYERPGSGELPDQPEHVALVCGSWPLHSWDREWE
                     LDLFDLKGVVEGVARELGVELEARPAELPGLLPGKAAELLRNGEVVGCLGEVAAKEFY
                     SLYVAEVALDALVSPGGQLSRQVVLPSRFPGIIADFTLTHALSTPWSEIRAAISAAQP
                     ADLEDFSLNVRYQGTLPDGVVNTTIRFEYNARDRSLTKDEVNARQADLTGMLVERFGW
                     KE"
     gene            12618..13007
                     /locus_tag="VEL74_15300"
     CDS             12618..13007
                     /locus_tag="VEL74_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63941.1"
                     /translation="MAAVAGIEWLEVLEEKVREASERLTNLRVENRGLEGRVRELEAR
                     LEALSGVAEGTDTMAAGLSAELERERRDSESLRERVRDLEARLAAADEGGASRDQVNE
                     LLQEREEVRGRVERLARHLEGLLAGSA"
     gene            complement(13029..14084)
                     /locus_tag="VEL74_15305"
     CDS             complement(13029..14084)
                     /locus_tag="VEL74_15305"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013554383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HYG63942.1"
                     /translation="MTLYSTLRRLLFGLDAETSHEIGIAGLEAIQALPSLARRLEERN
                     FVADERLGQTLFGRAFANPVGLAAGFDKNGVGIAALPALGFGFAEVGTVTPLAQPGNP
                     RPRLFRHPAARSLQNALGFNNQGMEALRRRLEAAWPARIPLGVNIGKNKTTPPEAALA
                     DYETLLRGLSPLCDYLVINLSSPNTPGLRDLQNEGFVRTLLGLAAGITAQPVLVKIAP
                     DLDPEAAAGLAETAVEAGAAGIVATNTTTDYALLPGARDSGGLSGEVLREKSFRVFDA
                     VARRLFGRTVLVSVGGIGSGAEAWRRIRAGASLVQVYTALVYQGPSLPRRINEELLAL
                     MERDGVKRLADAVGADR"
     gene            complement(14140..14682)
                     /locus_tag="VEL74_15310"
     CDS             complement(14140..14682)
                     /locus_tag="VEL74_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63943.1"
                     /translation="MRGEPVAPSFLTAIAAAVLLLLAGSAGAADLSFLWSDEANSTPA
                     TRPAAEKAGSCDGIGDGIWNGCRGTGCHVCDEKLAGYDCYFVNHPDCIRNTTCAGQFY
                     SCDSACPAPTAADRCAPTFNCNSYSNNPSLCLSMGCNLAPGFCAGNMDSHGNWCSDYW
                     YQSACFFAGGSCWWLAGWCY"
     gene            14878..15186
                     /locus_tag="VEL74_15315"
     CDS             14878..15186
                     /locus_tag="VEL74_15315"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HYG63944.1"
                     /translation="MEADVTTTQTPPSTEVEIFGEVYRVRGSDHEHLQGLAGLVDRRM
                     RDVARHANTADKARIAILVALNLADELSRVQKQQEGEQGEIREKVTALTEELSRALGG
                     "
     gene            15238..15425
                     /gene="ssrS"
                     /locus_tag="VEL74_15320"
     ncRNA           15238..15425
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VEL74_15320"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            15518..17104
                     /gene="rny"
                     /locus_tag="VEL74_15325"
     CDS             15518..17104
                     /gene="rny"
                     /locus_tag="VEL74_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HYG63945.1"
                     /translation="MVFTAIAFIGVLAVVLVWWFGLRRAARQILVQARHDAAKMAEDA
                     RRDAAKALEDAERQGETRLREAEIVAKEKLLQARSEFEAVSRKRRTELEALERRLTQK
                     EDNLDKRTEELTRREKELNQQDRSLAGREKVLEKKEGDLDRMLDEERVRLEQIAGLTA
                     QQAREELVRVMEDDARIEAAHIVKRIEDEAREQAHKQAQRIIGMAIQRSASDYVSEAT
                     VSVVMLPNDEMKGRIIGREGRNIRALETATGVDLIVDDTPEAVILSGFDPFRREIARV
                     ALERLITDGRIHPARIEEVVEKVKAEFEQRIHHEGEAALLELAIPGMHPELVKLIGRL
                     RYRTSYGQNVLQHSKEVAFLAGAMAAELKANVQVARRAGLTHDIGKAIDREMDGTHLQ
                     IGIDLLRKYGETEEVVHAMACHHGDYDPQTVEAVLVTAADALSAARPGARREILETYV
                     KRLEKLEEIASNFKGVQKVFAIQAGREIRIVVDSGKIGDEQALWLSKDIARKIEAELT
                     YPGQIKVTVIRETRSVEYAR"
     gene            17101..>17567
                     /locus_tag="VEL74_15330"
     CDS             17101..>17567
                     /locus_tag="VEL74_15330"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HYG63946.1"
                     /translation="MKLLFVGDVLGKPGRRAVQELLPRLVDRHRADYVVVNVENSAGG
                     FGVNPEVMKELEGLPIDCFTSGNHIWDKKEGVHLLDSDPRLLRPANYPEGNPGKGLYV
                     GETAAGIPVATINLEGRVFMNSLESPFVAADRLLSQLDPKVKVILVDFHCEAT"
BASE COUNT         2781 a         5789 c         6281 g         2716 t
ORIGIN      
        1 tgctcggggt gttgatctcg tttctgctgc gccgccggga catgctgacg ccgggcgcca
       61 agtcgctcct cggccagctc gtcctctggg ccgggatcaa cgtcgtctac ggcttcatca
      121 accccgggat cgacaacagc gcccacctcg gaggcttcgc cgccggcctc ctcctcggcc
      181 tcatcctgcg cgagcgccgg cgcttgcggg cgccggcggc ctatcccgag atcgaagcgt
      241 aggccgcccg cccggacccg ggcgggcctg ctatgcttcg gcgccttttg ggcaaaattc
      301 ttcgaggtct cgtatgaagc tttcgctcct gcaacggctt gccatgttcg cgagacaccg
      361 ctaccgcacg gtgttcgccg ctttcgtcgt cctggtcatc gtttcggccg ccctcatctc
      421 ccggctcagc ttcgacacgg acatgttgag cctcctgccg aaggaggacc cggcggtgcg
      481 gacctacgtg gagacgctcg aggacttcgg cagcagcacc tatctgctcg tcgcgatccg
      541 tatcccggaa ggggcggtgg tcgacccgta cgagaccctg gcggacgatc tggccgggcg
      601 gctggcacag ctgcccgagc tcaagagcgt gcaacacaag atcggcgagc cccaggagct
      661 cctggcgacc ttctatccga aggcgctgct cttcctcgac gacgccggcc ggcggcagct
      721 cgaggcgcgg ctctccgaca cgggcatcga acggcgggtg agcgagctcc ggcgccagct
      781 caagacgccg cagggcatgg ccgcgaagga gctgctgcgg ctggatcccc tggggatgtc
      841 cgacgtcttc ctgagccgcc tccagtcctc ccgcggcagc ctcagggtcg actggacgag
      901 cggctactac ctgtcgcgcg accaccgcct gctgctcatc ctggccgagc cggtgcggcc
      961 gccgcaggac atcgacttcg tcgagcggct cgccgtccag gtggaccgcg ccgtcgacga
     1021 ggcgcgcgga gagtgggagg agatcgccgg gccgggggtg aaggcgcccg aggtggtcgt
     1081 gggcggcccc catctcaccg cgctgggcga cgccgccctg atcaagaagg acatgatcct
     1141 caacctcggc accgcggtgc ccagcgtgct gctgttcttc tggttcacct tccggcgccc
     1201 gagctccctg atgtacgcgc tgctgccgat ggtcaccggc ctggccctca ccttcgcctt
     1261 cgccgagctg acgatcgggt cggtcagctc ggcgacgagc gtggtggcgg cgctgctcgc
     1321 cggcctgggc atcgacttcg tcatcgtctc ctacgggcgc tacatcgagg agcggcagaa
     1381 gggggcgacc ctggaggcgg cgctgctcgc gatgagcggc tccaccggcc gcgcggtgct
     1441 ggtcggcgcg gtcaccacga ccgtcacctt ctgggcgttc ctgttcaccc gcttcaccgg
     1501 cctgcgccag atgggcctgc tcaccggcac cggcatcctc ttctgcgtgc tgtccgtctt
     1561 cctgctgctg cccgccctgc tggcctgggg cgaggaccgc caccagcggc ggcggaccca
     1621 gcccaagctc tacctccaca gcttcggctc cgaggggctg atgcggctgt cgctgcgcca
     1681 cccctggccg gtctgcctcg ccggcatcgc catcaccctg gcggcgctgg cgctggcggt
     1741 caacatcgag ttcgacgaga gcatgaagac catgcggccg aaggggaacc ggggcatcga
     1801 cgtggccgcg gaggtcggca aggagttcgg ctccggcttc gattcgatga cggtggtgtt
     1861 gagcggcaag acgcccgagg aggtgatcga gctgtccggg cgggcggcgc acggggcgca
     1921 gcgcatggtc gacaccggca ccctctacgg cttcagcggc gtcacctccc tgatcccgtc
     1981 cccttcgcag cagcgggagg cgctcgcctg gatcgagcgc gagaaggggg acaccctcga
     2041 cctcgagcgg atccgcgcca cgttcgcccg cgccacggcg gcggaggggc tgcgcgccga
     2101 ggggttcacc gagggtttcg acctcctggc cagcgccgtc agcctgcggg agccgatcgg
     2161 ccccgagagc ttccaggaga gcccgcagac ccggctcctg ctcgagcgct acctcgtcaa
     2221 gacggaccgc ggctggaagg ccgccgtcta cctctatccg ctggacaacc ggtggcgccg
     2281 cgagccgccg ccggacgccc tccatctggt cgacaagctg gggccgcagg cggcgctgtc
     2341 gggcaccaac gtggtcaacc agcgcgtccg ctccctggtg ctgcgggacg cctgggtcgc
     2401 cgggatcatc ggcgtcgtcc tggtcgccct gctcatctgg atggatttcc gcagcctgcc
     2461 ccggacgctg gcctccctgg tgccgctgac ggtggggctg atctggatgg tgggcgccat
     2521 ggtgctggtg aacacgccga tgaacttcat caacatcttc gtgaccacca tgatcatcgg
     2581 catcggcgtc gactacggcg tccacatcct gcaccggttc caggaggtgc aggactatcc
     2641 gcgccgggag ttcgagaacg ccgtcgtcga gaccggcaag gcggtggtgg cggcgtcggt
     2701 ctccaccatc ttcggcttcg gctcgatcat gttctcgcac tacccggggc tggtctccac
     2761 ggggaaggtg gcggtcctgg gagccgtcac cacctgcctc gtctccatca ccctcgtgcc
     2821 cgccgtcctg gtctggcgct acgagcggcg gcggaagcgg gtgccggtgg gcgaggcggc
     2881 gtagggggcc gttcaggccc gtccggagca cgctgacttc gggccccagg gggcggtggt
     2941 gcctcccccc ctgggcgggt gggtggcagg gagcggggga ggtcaggtgg ggggtttttc
     3001 accgctccgc gttcctcgac tttcccgcaa gactcctcca tttccctctt gacgcgcccc
     3061 ccgtcgcgtt atagtctcgg gcggtcgtaa gccgtacaac gagaagggag gtggcagggg
     3121 aaagaaggaa tccgcggttc acgtcacagc cgtacaggac cgccgtgtct tcgaggtgag
     3181 cgcgtagctc atccggggcc tcggggttca agcggccaag ggagggagtt acgagctcgc
     3241 ctcccgaggc ctagccggac aggaggttgc actcatgccc agccacggtg atcgtacgcc
     3301 caacggcgtc gatctgtccc gtcccaccga atccacccgc cgtcatcttt acgagctggt
     3361 ggtccacgtc tacttccatc ccacgcctga agaggtggag aaggcccagt cggatcccgg
     3421 taaccactgg gtgccggact acggtcctga cgaagcacgc ctccacgtct tctatgccta
     3481 cgggcgctgg ttcgcatcct gggaggcgct ggaagagaag aagggggcgg cggccgatcc
     3541 ctcgcttccg gagtggcgtc gctggtcggt ggtgcggatc gtcgaggacg cggatccgtc
     3601 gagcgcgacg ggcgtcacct ttctggaagt ctgacgcccc cgccgtgacg tccgccgcct
     3661 cgcgcggccc gcctgaggac tgaaggaagc agcgccttct ccgccgtcgc gggggaggcg
     3721 cttttctctt ggcggggatt gcggcggcgg atcggaagct ccggcagcgc gccggaagct
     3781 ctgccacctc gccgaagctt ccgatgcgcc ccccgcgcgt cgactgagcc tgccctgggg
     3841 ggttggacgc gatccggagc gctcgggaag cttccgggtg ccgcggaatc catccctttg
     3901 aatcgggcgc catccgcggc gtccgggaag catccgaagc gtctggaatc catcccgtcg
     3961 aatggggagc cgtccgcggc gcgcgggaag cgtccgaggc gtctggacac catccgccgc
     4021 gctttggaca ccatccgcgg cgtccggacg ggatccacgg cgcctggatg agctccggaa
     4081 gcccgggctt cgagtcttcg cgtccgcggc ggatcgcttg ggggttgggg gaaggggggc
     4141 cgtccgcccc aaaagaaaag ggccgccggg ttgccccggc ggcccctcct aggttactgc
     4201 aggaaggatc agaagtcgaa ggcgaccgtc accaggtacg cccgcggcgg ctgatagtcg
     4261 agctcgttgc ggatacggcc gaaggaggag cagttgcggt tcggctcgtc gtcgagaccg
     4321 caggtgccgg ccggcgccca gctccgggtg ccatcggcgt tgaggaccgt ggtgccggtg
     4381 gtctggaacg cgcggagctc gtcgttgtcg agcacattgg tgaccgccag cttcaggaag
     4441 acgttgaagt cgctgaagat gggcagctcg taacgggccg acaggtcgag ctgccaccag
     4501 tcgttgaacc gctcggagcc acgctcaccg aagaagcggg tgaaggtgcc cggggggttg
     4561 ccgccgctga tgtagcccgg agtcgcggtg tacggccggc tggccgtccg gctccagttg
     4621 cggcccgact ggtagtagac gatggagccc agggtgaggg cgccagcgcg gttgaagtcg
     4681 aaggagtagg tgccgagcag gttgacgcgg tgacggatgt cgtcatcggt gtagccgtac
     4741 ggcgagctgt tgatgaccga cagggggtag ttgcccaggt tcgagccgga ggacggctgg
     4801 ttgccagcct caccctcgta gttaccctcg gtggtcgagt aggtgtagtt gccggagagc
     4861 gaccagcggc tggtcggacg gtagtcgaag accgccgtca gagcgcggta ctcgcgcacc
     4921 gcgtccgggt tgttggccca gatggtggtg tcgagcggga aggccgcgcc gccgcccggg
     4981 gccacgacgg aggtggtgtc acccgcgtcg cacaggccct ggttggtgca gaccaggccg
     5041 atgaagtcgt cgagcatgtc ctcgtagtcg cggcggatgt aggagagaac caccgaaccc
     5101 gagttgcgcg gcagggcgtg ccggatcgac aggttgtagt cgtcggcgta gggggcgttg
     5161 atgtcgtcgg ccaggaaggt cgtcggctgc gtcggatcga tgtacgacgt gacgatcgtc
     5221 cagaactggt cgtcgcgcag gatctgctgc acctggtcgg cggtcaggcc ggagaggtcc
     5281 ggaccggtgt agaggtgctc gatacgcggc gcgttggcga cgcccgtggc attgttggcg
     5341 acgttgtcgt tgaaacggct gacatagcgg ccgtacgtcg cctgcacctg ccaggacgga
     5401 gtgatgctgt aggtcacgcc caggcggggc gcgatgtcgt cgaaggacag gttgaactgc
     5461 gccagcgggc cggagccgtc gtactggtca taccgtacgc cgatgtcgaa ccgccagttg
     5521 ttgagcgtcc aaccgtcctg gaggtagaca cccaggttgt cgagctgctg gtcgccgccg
     5581 agaggcacgg cgagccaacg gtacatgtaa ccggtctcga agttgtaccg gggggtgccg
     5641 ccgacctgac cggcgtagaa gcggccggtg ttcagggcga gcaggttgta gccggtcgag
     5701 gactgacggt tctcaccacc ggtggtggag cggacgaact gcacgccgcc ctccagggtg
     5761 tgggagccga ggctctcggt cgacagggag tgcgtgaggt tgagggccgc cgtctcgttg
     5821 tcgcggatgc tgggatcggt gaaatcccac cagccgttgt tgtagacggt gttggtgaag
     5881 tactcgatga acgggtcgcc cttggcgggg tcgccgcccg agttgatctc gacgttcttc
     5941 tgggtcgcct ggaactccag gaaggtgttc gagcccagaa cgccctggta agaggcggtg
     6001 atcgtctcgc gcgggtcggt acgggtaccg acggcacgcg cctcaccggg ggtgaggccg
     6061 gcctggttca gagactccga gtcgaagctg aggtagctca gatcgagcag gtggttctcc
     6121 gagagagcgc cgcgcagctt cgcctgccaa cggtcctcgt tgtaggtgtt ggtgatcgcc
     6181 tcaccggtgc cgctcgccgt acgcgaggtc tgcgactccg ggatggtgcg gccgccgacg
     6241 aagaaccaga ggcggtcctt cacgatgtac ccaccgagcg tcgcctggta ggtctcctga
     6301 aggtcgtcct cccgcacctc gttgaatggc gaggacgagt tccagtcctc gttctcgagc
     6361 tccgcgcgca gcgtgccctc gaactcgttg gaaccggact tggtgacggc attgatgatg
     6421 ccgccctgga aacggccgta gcgggcgctg acgcccgagg tcaggacctg cacctcttca
     6481 acggcatcct ccaggtagag gtccggggcc gaaccgaagt acgggtcgga cacctcggca
     6541 ccatcgagca tcaccgtcgt gtcgaacgac ggcgcacccg agatcgagag cgtgccgccg
     6601 gtcgggccga acgagatgtt cggcgacagg gtggcgacgg actcgatgtc gcgggcggtg
     6661 atcggcaggg cctcgacctc ggtctcgtag tcgaagttct cgccgatcgt ggtggtttcg
     6721 agggcagtcg ccacgccggt gacggtgatc tcctccgata ccacctgacc cggagcgagc
     6781 tgggcgttga ccggaacggt ctggcccagc tccaggcgga agcggtacac ctccggctgc
     6841 cggccttccg aggccaccgt gacctcgtag ttgccggtcg gcagcagggc gaacaggaag
     6901 tcgccgttct gcccggtggt cgccgtgcgc tcgagctgga gagactggtt gttgacggtg
     6961 accgtcgcgc caggcagcgg cgcgccgttc tcgtccgtca cccggccatt gatccggccc
     7021 gtcgtctgag cctgagcaag gaccagggcg ggcaccatca acagcaccag caccagccac
     7081 aggtttcgtt tcatgccttc ctccttgtgg tttggtgaac acttttcccc gaccgggatc
     7141 ggggaaacct ctttaacgac cggggctctt cgccccgtcc aactcccgag atagcacttt
     7201 ccatgcctgc cgggaagaat ggccaactta ctggaaaaga accacttgcg agtttcagca
     7261 gcgccggagt ccaacggcgc ggatcagaga tttgaatcca gatccagaaa tctggatgcc
     7321 ggccacccgg ccagcccccg gggccgccgg cccggcggga ccggatggcg cggggggcgg
     7381 tgtgctggcc gttgacaccc ccggagaggt ctgctagtct ctctctccct cgggcggccg
     7441 tcgttggcgg gtgtccgtgg gctagtatag acgaagagaa ggcccgtagc tcagttggtt
     7501 agagcgctac gttgacatcg tagaggtcag cggttcgaat ccgctcgggc ctaccatcgt
     7561 agagattgta gagatcgtcg agatcgttgc agggaacacg aagatcgaca cagagtgttg
     7621 gatggcatgt ccaggcgctt agctcagctg gttagagcgc taccttcaca cggtagaggt
     7681 cagcggttcg aatccgctag cgcctaccaa ctctctctct ccccgtcgcg ctcgtgagtc
     7741 cccgctccgc ggccccgctg cccgggctcc tccccgtgtc cccttccctg ccgccgccgg
     7801 aatccggcgt gctgccgtgc taggcttgga tgcagtgtct cttcgcgatt tctggccggg
     7861 ccggcagggg gtgctctccc cctggccgct cgcgtaccta tataggaaga gagcccgccg
     7921 acgggccatt gaacgacctg ccggacccac caccgtggtc cggcgctccc agggggtgag
     7981 ctgagtatcg acaagacgac gcggatcaac gagcggatca gggtgcggga ggtgcgcctg
     8041 gtggagtccg acggctccca ggcggggatc gtaaccatcg aggacgcgct gcagcgcgcg
     8101 cgcacccagg agctcgacct cgtggaggtg gcgcccacgg ccaagccgcc ggtctgccgc
     8161 atcatggact tcggcaagtt cctctatcag cagaagaaga aggcgcacga gtccaagaag
     8221 aagcagaagg tgatccacat caaggaggtc aagttccggc cgaacatcga cgaccacgac
     8281 tacgacttca agctgaagaa cgtggtccgg ttcctcggcg aaggggacaa ggtcaaggcg
     8341 accatccagt tccggggccg tgagatggcg cggcaggacc tggggcacaa gctggtcaag
     8401 cggctccagg ctgacctcgg ggagtcgggg gtgctagaat cccaccccga gatgacgggt
     8461 aaccggatgc atgtcatctt cggaccgccc cgcaatcagg tgcaggtggc ggcggcgccg
     8521 aagagggcag cgcccgccgg cgagcgcgcc gagcgccctg agcgcagcga cgcccgcgcc
     8581 gagaacccgg agccccgccc ggcttccagc taggcggtgg atgacgcaga gccggccctc
     8641 ggagcgccca tggtgggcgg ctccccgggg gccttacgcg ctttcggcag agtccgaggc
     8701 gccagaaagg agagggaccc ttgcccaagc agaagacgca caagggtgcc gcgaagcggt
     8761 tcaagttgac ggccaccggc aaggtgaagc ggcatcagtc gatgcacagc cacatcttga
     8821 ccaagaagac gaccaagcgg aagcgcaatc tccgcaaggc gacgatggtc tcgcccgcgt
     8881 tcgaaggcat catcaaggac ctgttgcact cgtagtctcc ggcctgccgg gaccgcatca
     8941 aacccaaccc tcggccgccg gcgggacgac ctgtccgccg gagccgtagc ccccggcttc
     9001 cgggatcgca aggatcccga tagctcgcgc gggcctgacc gctccaccct ttcgacaagt
     9061 gtgggagcag aaacgaggtc atacagatgg cacgtatcaa gcgcggcaac aagcgcgtcc
     9121 agcgccgcaa gaagatcctg agcctggcca gcggcttctg gggcatgaag tcgaagagcc
     9181 accgcatggc caagcaggcg gtggacaagg ccctcaacta cgcctaccgc gaccgccgcg
     9241 tccgcaagcg cgagatgcgc agcctgtgga tcgtgcgcat caacgccgcg gcccggctgc
     9301 acggcctgtc ctacagccgc ctgatcgccg gcctcaaggc ggccggctcg gacctcgacc
     9361 gcaaggtcct ggccgacatc gcggtgcgcg acgccgaggc cttcggcggc atcgccgaga
     9421 tggccaagaa agctctcggc aacaagcagc agtccaagcc cgcggaggcg tgaggtgacg
     9481 gacggcggca cctccgcggc ccccgccccc gccgatcggg atagcgggac ggccgccggt
     9541 gccgcccggc cggccgacct cgccgcgcag ttcgagcgcg aggccggcgc cgtggcggac
     9601 cggcaggcct gggaagagct gcgcctgcgc tgggtgggac gcaagcaggg gatcgtccgc
     9661 tcgctcctcg acgacatcaa gaaggtggag ccggccggcc gccgcgccta cggcgaggcg
     9721 gtcaacacgc tcaaggagac ggtggaggcc cgcctcgccg cgctggacga ggagatcggc
     9781 cggcgcgagc gggaggcggc gctgcgccgc tccacggtcg acgtcaccct gccgggccgg
     9841 cgccccgcca tcggctcgat ccaccccatc accctggtga tgtgggagat ggaggagatc
     9901 ttcgcctcgc tgggctacag cgtcgccgag ggccccgagg tggaggacga ctaccacaac
     9961 ttcggcgccc tcaacttccc cgacgaccac ccggcgcgcg acacgcagga cacgctgttc
    10021 ctcgacaacg gcctgctgct gcgcacccac acgtcgccgg tgcagatccg caccctgctg
    10081 tcgcgcaagc cgccgatccg ggtgatctgc ccgggccggg tcttccgcaa cgacaatgac
    10141 ctgcggcact cgcccatgtt ccatcaggtg gaggggctgg cggtggccga ggggatcacc
    10201 gtcggccatc tcaaggggac gctccaggcc ttcctccagc ggctgttctc gccggagacg
    10261 cagatccgcc tgcgccccag cttcttcccc ttcaccgagc cgtcggccga ggtggacgtc
    10321 acctgcgcca actgcaaggg caaggggtgc gaggtctgct ccggcaccgg ctggatggag
    10381 atcctcggct gcggcatggt cgacccgcgg gtgctcgccg ggtgcggcat cgatccggac
    10441 gtctacagcg gcttcgcctt cggcatgggg gtcgaccggg tggcgatgaa ccgctacggc
    10501 atccccaaca tccgcatgct gttcgacaac gacgagcggc tgctgcggca ggtgcgcgaa
    10561 tgaagttctc tctggagtgg ctgtccgact acgtggagct gccggcggag ccgcgggagc
    10621 tggccacgcg cctgaccgcc gccggcctgg cggtcgagga tgtcgaggac ggtgagcacg
    10681 ggaccctctt cgacatcgac gtcaccacca accggcccga ctgcatgaac cacttcggcc
    10741 tggcgcggga gatctccgtc ctgctggaga agccgctgcg ccggccggcc gcggaccccg
    10801 ccgaggcgac ggagcgggcg gcggacgcgg cgcgggtggt gatcgaggac tccgaagggt
    10861 gcccgcgcta tgccgcgcgg gtgatccgcg gcgtcaaggt ggggccgagc cccgactggc
    10921 tgcgccggcg cctgcaggct gtcggcctgc gctcgatcaa caacgtggtc gacgtcacca
    10981 actacgtgct ctgggagctc ggccagccgc tccacgcctt cgacctggcg aagctcgccg
    11041 gggcgacggt cgtggtgcgc cgggcgcggg cgggggagcg gctcgtcacc ctcgacggcg
    11101 ccgagcgcga gctcgacccg gagatgctgg tgatcgccga cgccgagcgg gcggtggcgc
    11161 tcgccggcgt catgggcggc gccgacagcg aggtgacgga cgggaccacc gatatcctgc
    11221 tcgagagcgc ccacttcggc cgccggcggg tgcgccaggc ggcgcggcgg ctcggcatcc
    11281 actccgacgc cagccaccgc ttcgagcgcg gcgccgaccc ggagatctgc gccgaggcgg
    11341 cgagccgggc ggcgaagctg atcgccgagg tggcgggggg cgaagtgctc gccggcgtca
    11401 tcgacgtgcg cgccgccgag gtgccggcgc gccgcggccg gctggacctg gaccggctcg
    11461 acgccttcgc cggcgccgcg gtgccggccg ccgacgccga gcgctggctc accggcctcg
    11521 gcttccggct ggagccggac ggtgaacggg cctggcgggt gacggtcccc tcctggcgga
    11581 cgtacgactt cgagccgcgg ccgtcgggcg agatctatcc ggccgacctc tatgaggagg
    11641 tgctgcgggt cttcggcctc gaccgcatcc ccgcggcgct gccggggatc ccgggcccgg
    11701 acgcgccgag ggcggagaac caggtgcggc gcacgcgggt gcggcgctac ctcgccgccg
    11761 ccggcttcgc cgaggcggtc cacttcgcct tccacgaccc ggcggcggac gcggcctatc
    11821 cgagcctgcg gccggacacg aagccgctgc cgctggccaa cccgctgtcc gagcgctatt
    11881 cggtgatgcg gcgctcgctg atccccaacc tggtggagag cgcccgcttc aatcagcgtc
    11941 ggggacgcga gtcggtccgg ctattcgagg tggcgacggt attctacgag cggcccggat
    12001 ccggagagct gccggaccag cccgagcacg tggcgctggt ctgcggaagc tggccgctcc
    12061 attcatggga ccgagagtgg gaactagacc tgtttgacct taaaggggtg gtggagggcg
    12121 tggcccgtga gttgggcgtc gagcttgagg cacggccggc cgagctgcca ggccttctcc
    12181 ccggcaaagc ggccgagctg ctgcgaaacg gggaggtggt ggggtgcctg ggtgaggtcg
    12241 ctgccaagga gttctactcc ctttacgtgg cggaggtggc tttggatgct ctggtgtcac
    12301 caggcgggca gctgagtcgc caagtcgtct tgccctcgcg ctttccgggg atcatcgcgg
    12361 acttcacgct gactcatgcg ctatcaaccc catggtcgga aatccgtgca gccatctcgg
    12421 cggcgcagcc ggcggatcta gaagacttca gcctcaacgt gcgttatcaa ggcacgctgc
    12481 cggatggggt ggtcaacaca acaatccggt tcgaatacaa cgcgcgcgac cgctcgctga
    12541 ctaaggacga ggtcaacgcc cgccaggcgg acctcacggg catgctggtg gagcgcttcg
    12601 gctggaagga gtaaggcttg gcagcagtgg cagggatcga gtggttggag gtgctggagg
    12661 agaaggtccg ggaggcgtcc gagcgcctga ccaacctgcg ggtcgagaac cgtgggctgg
    12721 aggggcgggt gcgggagctg gaggcgcggc tcgaagcgct ctccggcgtc gccgagggca
    12781 ccgacacgat ggccgccggc ctctccgccg agctggagcg cgagcgccgg gacagcgagt
    12841 cgctgcgcga gcgggtgcgg gatctcgaag cccgcctcgc cgccgcggac gaggggggcg
    12901 cctcccgtga tcaggtgaac gagctgctgc aggagcgcga ggaggtccgc ggccgggtcg
    12961 aacgcctcgc tcggcatctc gaagggctgc tggccggcag cgcctagcgg tccgctccga
    13021 ctgacctcct agcggtcggc cccgaccgcg tcggccagcc gcttcacccc gtccctctcc
    13081 atcagggcga gcagctcctc gttgatccgc ctgggcagcg acggcccctg gtagaccagg
    13141 gccgtgtaga cctggaccag cgaggcgccg gcgcggatgc ggcgccacgc ctcggcgccc
    13201 gagccgatgc cgccgacgct caccagcacc gtccgcccga acaggcgccg ggccacggcg
    13261 tcgaacaccc ggaagctctt ctcgcgcagc acctcccccg acaggccgcc ggaatcgcgg
    13321 gcgcccggca gcagggcgta gtcggtggtg gtgttggtgg ccacgatgcc ggcggcgccc
    13381 gcctccaccg cggtctcggc gaggccggcg gcggcctccg ggtcgaggtc cggggcgatc
    13441 ttgaccagca ccggctgcgc ggtgatgccg gcggcgagcc cgaggagcgt ccgcacgaaa
    13501 ccctcgttct ggaggtcgcg caggcccggc gtgttgggcg acgagagatt gatcaccagg
    13561 tagtcgcaga gcggggagag gccgcgcagc agcgtctcgt agtcggcgag cgccgcctcc
    13621 ggcggcgtcg tcttgttctt gccgatgttg acccccagcg ggatgcgggc gggccaggcg
    13681 gcttcgaggc ggcggcggag cgcctccatc ccctggttgt tgaagccgag ggcgttctgc
    13741 aggctgcgcg ccgcggggtg ccggaagagg cgcggccggg ggttgcccgg ctgggcgagg
    13801 ggggtgaccg tccccacctc ggcgaagccg aagccgaggg cgggcagggc ggcgatgccg
    13861 acgccgttct tgtcgaagcc ggcggcgagc cccacggggt tcgcgaaggc gcggccgaag
    13921 agcgtctggc cgagccgctc gtcggcgacg aagttccgtt cctccagccg gcgggcgagc
    13981 gacggcagcg cctggatcgc ctcgaggccg gcgatgccga tctcgtggct ggtctcggcg
    14041 tcgagcccga agagcaggcg gcggagggtg gagtatagcg tcatgggcgg cgggggcgat
    14101 tctagaccgg atcgcccccg ccgtagtcgt ggtctgtcgt tagtagcacc accccgcgag
    14161 ccaccagcag gagccgcccg cgaagaagca ggccgactgg taccagtagt ccgagcacca
    14221 gttcccgtgg ctgtccatgt tgccggcgca gaatcccggc gcgaggttgc agcccatgct
    14281 caagcagagg ctcgggttgt tcgagtagct gttgcagttg aacgtgggcg cgcagcggtc
    14341 cgccgcggtc ggcgccggac aggccgagtc gcaggaatag aactggccgg cgcaggtggt
    14401 gttgcgaata cagtccgggt ggttgacgaa atagcagtcg tagccggcga gcttctcgtc
    14461 gcagacgtgg cagccggtgc cgcggcagcc gttccagatg ccgtcgccga tcccgtcgca
    14521 gctcccggcc ttttcggccg ccgggcgggt ggccggggtc gagttcgcct cgtcggacca
    14581 gaggaagctg aggtcggccg cgccggcgga gccggcgagg agcaggagca ctgcggcagc
    14641 gatggcggtc aggaaggacg gagctacggg ctctcctctc atgggggtct cctcgggagt
    14701 agatctctgc agatgattct gcagagcagg gacatctaaa aggtgaggtg cgccggacgg
    14761 ccggaatgtg gcgccgcgcg gcccctcgac gccctctctg gccccgctcc tcgactgtgc
    14821 tatagtgggc tccgccaagt ccctggcatc acatcacttg ttggccgggg aaggcctatg
    14881 gaagcggacg tgaccacgac tcaaacccca ccttcgaccg aggtcgagat ctttggtgag
    14941 gtctatcgcg tccgcggtag cgaccatgag cacctccagg gtctcgccgg cctcgtcgac
    15001 cgcaggatgc gggacgtggc ccggcacgcg aacacggccg ataaggcacg gatcgcgatc
    15061 ctggtggcgt tgaaccttgc ggacgaattg tcgcgagttc agaagcagca ggaaggggaa
    15121 cagggcgaga tccgggagaa ggtgacagcg ctgacggagg aattatcccg tgccctcggc
    15181 ggttgatgag ggggcagcag cgctcggcgc cagctcgtga tatatggaaa caggtttgca
    15241 cccctgcgcg gttcgtgatg ggtcaaccga ttgtagaacc aacattgtta ccatgggagt
    15301 tctataccca cgtggcctgt gccagcctcg caccgacgag gacgctaacg gctcgtggtg
    15361 cggacaccca cctggcgcgt tcaggttcta gacgcgacgg ctcagcacgg ccgcgcgggg
    15421 gatgcttaat ccctctctgg tgagagggtc ccttccagcc tcgtccggtc ccccctgtcg
    15481 ctcctgacag ggggtatatt cggtggatac cgacggaatg gtattcacgg ccattgcctt
    15541 tataggggtt ttggccgtgg ttctggtctg gtggttcggc ctgcgcaggg ccgcccggca
    15601 gatccttgtc caggcgcgtc acgacgccgc caagatggcg gaagacgccc ggcgtgacgc
    15661 cgccaaggcg ctggaggatg ccgagcgcca gggcgagacc cgcctgcgcg aggcggagat
    15721 cgtcgccaag gagaagctgc tgcaggcgcg cagcgagttc gaggcggtct cgcgcaagcg
    15781 ccgcaccgag ctcgaagcgc tggagcggcg cctcacccag aaggaagaca acctcgacaa
    15841 gcgcaccgag gagctcaccc ggcgggagaa ggagctcaac cagcaggacc ggtcgctggc
    15901 cggccgcgag aaggtgctgg agaagaagga aggcgatctg gaccgcatgc tcgacgagga
    15961 gcgggtgcgg ctggagcaga tcgccggcct gaccgcgcag caggcgcgcg aggagctggt
    16021 gcgggtgatg gaggacgacg cccgcatcga ggccgcccac atcgtcaagc ggatcgagga
    16081 cgaggcgcgg gagcaggcgc acaagcaggc gcagcgcatc atcggcatgg cgatccagcg
    16141 ctccgcctcc gactacgtct ccgaggccac cgtctccgtg gtcatgctgc ccaacgacga
    16201 gatgaagggg cggatcatcg gccgcgaggg ccgcaacatc cgcgccctcg agacggcgac
    16261 cggcgtcgat ctgatcgtcg acgacacgcc cgaggcggtc atcctctccg gcttcgaccc
    16321 gttccgccgc gagatcgccc gcgtcgccct ggagcggctg atcaccgacg gccgcatcca
    16381 ccccgcccgc atcgaggagg tggtggagaa ggtcaaggcc gagttcgagc agcgcatcca
    16441 ccacgaaggg gaggcggcgc tgctggagct cgcgatcccc ggcatgcatc ccgagctggt
    16501 caagctcatc ggccgcctgc gctaccgcac ctcctacggc cagaacgtgc tgcagcacag
    16561 caaggaggtc gccttcctcg ccggcgccat ggccgccgag ctcaaggcca acgtccaggt
    16621 ggcgcgccgc gccggcctca cccacgacat cggcaaggcc atcgaccggg agatggacgg
    16681 cacccacctg cagatcggca tcgacctgct gcgcaagtac ggggagaccg aggaggtggt
    16741 ccacgcgatg gcctgtcacc acggcgacta cgacccgcag acggtggagg cggtgctggt
    16801 gaccgcggcc gacgcgctgt cggcggcgcg gcccggcgcc cggcgcgaga tcctggagac
    16861 ctacgtcaag cggctggaga agctggagga gatcgcctcc aacttcaagg gggtgcagaa
    16921 ggtcttcgcc atccaggccg gccgcgagat ccgcatcgtc gtcgacagcg gcaagatcgg
    16981 cgacgagcag gcgctctggc tgtccaagga catcgcccgc aagatcgagg cggagctgac
    17041 ctatcccgga cagatcaagg tcacggtgat ccgcgagacc cgctcggtgg agtacgcccg
    17101 gtgaagctgc tcttcgtcgg cgacgtcctc ggcaagcccg gccgccgggc ggttcaggag
    17161 ctcctccccc gcctggtcga ccgccaccgc gccgactacg tggtggtcaa cgtcgagaac
    17221 tcggccggcg gcttcggggt caatcccgag gtgatgaagg agctcgaggg gctgcccatc
    17281 gactgcttca ccagcggtaa ccacatctgg gacaaaaagg agggggtcca cctgctggac
    17341 agcgaccctc ggctgctgcg ccccgccaac tatccggagg ggaaccccgg caaagggctc
    17401 tacgtgggag agacggcggc gggcatcccg gtcgccacca tcaacctcga ggggcgggtg
    17461 ttcatgaaca gcctggagtc ccccttcgtg gcggccgacc gcctgctctc ccagctcgac
    17521 cccaaagtca aggtgatcct ggtggacttc cattgtgagg cgaccag
//
LOCUS       DASPJI010000553         3375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_497316, whole genome shotgun sequence.
ACCESSION   DASPJI010000553 DASPJI010000000
VERSION     DASPJI010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497316"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2516
                     /locus_tag="VEL74_15335"
     CDS             <1..2516
                     /locus_tag="VEL74_15335"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYG63947.1"
                     /translation="EPVGGGGMGIVYKARDTRLGRTVALKFLPPELTRDPEAKERFLQ
                     EARAASALDHPNVCTVHEIGEDEEGRLFLAMAFYDGETLKRRLERGPLPLDEATDVAK
                     QVAQGLTKAHRQGIVHRDVKPANLMLTGDGVVKVLDFGIAKLAGSSGLTQAGSSLGTP
                     AYMSPEQARGEEVDGRTDVWSLGAVLYEMLAGRRPFPGDHDQAVIYALLHETPKPLPS
                     LRVDVPPDLARIVARMLEKDPERRCPSMADALADLRAFRNEPGTGTVQRAAAPPARRR
                     WPWLAAGVVAVLAAGGLYLALGAREEVGQAQFERLTEGGGRELFPTLSPDGNSFAYVK
                     AGEGNLDIYLQSVGGDNPLRLTDSLADDTQPAFSPDGRQIAFRSEREGGGIFVMGNLG
                     GSSRRVSEIGFNPAWSPDGEELVVATEGVSGPLARTGRSELWRIVAATGERRLVTRGD
                     AVQPSWSPNGHRIAYWSVSSKSAERTVWTVPAAGGEARRVTNDTFLNWSPVWSPDGRY
                     LYYSSDRSGTPNIWRVPIDEASGEVRGAPEAINAPSESSSFMSFSADGRRMIYATDDG
                     RSNLEKIGFDPGRLAVEGELSAVTEGSRSIFTGEASPDGRWLAYQVTQPRDDLFVIQP
                     DGSEPRQLTDDDAKDRLPRWAPDGSRILFYSNRSGRYGAWTIRPDGSGLEQLTVDTGE
                     PLYNPLWSPDGRRIVFILGFKGTALLDLDDLSRRPKPLPLPVGTGVFNATSWSPDGRF
                     LAGELTRPGIAVYSLDSGRLDRLTDSGTSPVWLAGGRALLYLDRGRISAFDLRGRTVR
                     EVLSPRANSEFRSVSPGPNSRTLYLVRATFEGDVWQRTN"
     gene            2598..2864
                     /locus_tag="VEL74_15340"
     CDS             2598..2864
                     /locus_tag="VEL74_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63948.1"
                     /translation="MAFDSRDVMISLPSAGEGVGYQLTCRPLSPQAPPPDEKGKPKPP
                     CPAPSCQNSTRGPKPPLKPGPKKRTAAGLLALRQQLRQTLSREQ"
     gene            2911..>3375
                     /locus_tag="VEL74_15345"
     CDS             2911..>3375
                     /locus_tag="VEL74_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63949.1"
                     /translation="MSMADDFSGRTLRANPLYELVPLERLDEAERRAMARLDGGLEED
                     PATYGILRPRDAAAGLAVKSADRDTALLFLTLRDPGPLPAYVRRAFGQPGLRAVTRLV
                     ADGILEISEKDGFLSGAAALARLTGPRDQAAPGSRLAELSLSALRYGHSLTLE"
BASE COUNT          462 a         1314 c         1158 g          441 t
ORIGIN      
        1 tggagccggt gggcggcggc ggcatgggga tcgtctacaa ggcgcgcgac acgcggctgg
       61 ggcggaccgt ggcgctcaag ttcctccccc ccgagctgac ccgcgacccg gaggccaagg
      121 agcgcttcct ccaggaggcc cgggccgcct ccgccctcga ccaccccaac gtctgcaccg
      181 tccacgagat cggcgaggac gaggaggggc gcctcttcct cgccatggcc ttctacgacg
      241 gcgagaccct gaaacggcgg ctggagcgcg ggccgctccc cctggacgag gcgaccgacg
      301 tcgccaagca ggtggcccag gggctgacca aggcgcaccg ccaggggatc gtccaccgcg
      361 acgtcaagcc ggcgaacctg atgctcaccg gcgacggcgt ggtgaaggtc ctcgacttcg
      421 gcatcgccaa gctcgccggc tcgtcggggc tcacccaggc cggctcgtcg ctcggcacgc
      481 cggcctacat gtcgcccgag caggcgcggg gggaggaggt ggacggccgc accgacgtct
      541 ggtcgctcgg cgccgtcctc tacgagatgc tcgccggccg gcgccccttc cccggcgatc
      601 acgaccaggc ggtgatctac gcgctccttc atgagacgcc gaagccgctg ccgtcgctgc
      661 gggtggacgt gccgccggac ctggcgcgga tcgtcgcgcg catgctggag aaggacccgg
      721 agcggcgctg cccctcgatg gccgatgccc tcgccgacct gcgggctttc cgcaacgagc
      781 cggggacggg gacggtgcaa cgggcggcgg ccccgccggc ccggcggcgc tggccgtggc
      841 tggcggccgg cgtcgtggcg gtcctggccg ccggcgggct ctatctggcc ctcggcgcgc
      901 gcgaggaggt gggccaggcc cagttcgagc gcctcaccga ggggggaggg agggagctgt
      961 tccccaccct ctccccggac ggcaactcct tcgcctacgt caaggcgggc gaggggaacc
     1021 tcgacatcta cctgcagagc gtcggcggcg acaaccccct gcggctcacc gactccctgg
     1081 ccgacgacac ccagccggcc ttctcccccg acggccggca gatcgccttc cggtcggagc
     1141 gggagggcgg gggcatcttc gtcatgggga acctcggcgg ctcgtcgcgc cgggtgagcg
     1201 agatcggctt caaccccgcc tggtcccccg acggcgagga gctggtggtc gcgaccgagg
     1261 gggtgagcgg cccgctggcg cggacgggcc gcagcgagct ctggcggatc gtcgccgcca
     1321 ccggcgaacg gcgcctggtc acccgcggcg atgccgtgca gccgagctgg tcgccgaacg
     1381 gccaccggat cgcctactgg agcgtcagct cgaagagcgc cgagcgcacc gtctggaccg
     1441 tgcccgccgc cggcggcgag gcgaggcggg tcaccaacga caccttcctc aactggagcc
     1501 cggtctggtc gccggacggc cggtatctct actactccag cgaccgctcc ggcaccccga
     1561 acatctggcg ggtgccgatc gacgaggcca gcggcgaggt gcggggggcg ccggaggcga
     1621 tcaacgcgcc ctccgagtcg agcagcttca tgagcttctc cgccgacggc cggcggatga
     1681 tctacgcgac ggacgacggc cggtcgaacc tggagaagat cggcttcgac cccggacggc
     1741 tggcggtcga gggggagctg tcggccgtca ccgagggctc gcgctccatc ttcaccggcg
     1801 aggcgtcgcc cgacggccgc tggctcgcct accaggtgac gcagccgcgg gacgacctgt
     1861 tcgtcatcca gccggacggc tcggagccgc ggcagctcac cgacgacgac gccaaggacc
     1921 ggctcccccg ctgggccccg gacggctccc gcatcctctt ctactccaac cgctccggcc
     1981 gctacggcgc atggaccatc cgtccggacg gcagcggcct cgagcagctc accgtcgaca
     2041 ccggcgagcc gctctacaac ccgctctggt cgcccgatgg acgccggatc gtcttcatcc
     2101 tggggttcaa gggcacggcg ctcctcgacc tggacgacct cagtcgccgc cccaagccgc
     2161 tccccctgcc ggtcggcacc ggagtgttca acgccacctc ctggtcgccg gacggccggt
     2221 tcctggccgg cgagctcacc aggcccggca tcgccgtcta ctccttggac agcggccgcc
     2281 tcgaccggct gaccgacagc ggcaccagcc cggtctggct cgccggcggc cgagcgctcc
     2341 tctacctgga ccggggccgg atctccgcct tcgacctgcg cggccggacg gtccgggagg
     2401 tcctgtcccc gcgcgccaac tccgagttcc ggagcgtgag ccccgggccg aacagccgca
     2461 ccctctatct ggtacgcgcc accttcgaag gggatgtctg gcagcggacg aactgagagc
     2521 cctcgctggg ctatacttcc tgtcgatcag caacagtttg ccgcactgcc cggggacctg
     2581 tccccgaagg agaggagatg gcgttcgatt cgagggatgt gatgatcagt ctgccttcag
     2641 cgggcgaggg ggtgggctac cagctcacct gcaggcctct gtccccccag gcgcctcccc
     2701 ctgacgaaaa gggcaagccc aagcccccct gccccgcgcc aagttgccag aactccacgc
     2761 gcgggcccaa gcccccgctc aagccgggcc ccaagaagcg gaccgccgcc ggcctcctgg
     2821 cgctgcgcca gcagctccgg cagaccctct cccgggagca gtagagagga gaccgcgccc
     2881 cggcggcgcc accccgccgc cgggcctctg atgtccatgg ccgacgactt ctccgggcgc
     2941 accctccgcg ccaacccgct ctacgagctg gtgcccctcg agcggctgga cgaggccgag
     3001 cgccgcgcca tggcccgtct ggacggcggc ctggaggagg acccggccac ctacggcatc
     3061 ctgcgccccc gggacgccgc cgccggcctc gccgtcaagt ccgccgaccg tgacacggcg
     3121 ctcctcttcc tgaccctgcg cgaccccggc ccgctccccg cctatgtgcg gcgtgccttc
     3181 ggccagcccg gcctgcgggc cgtcacccgc ctggtggccg acggcatcct cgagatctcg
     3241 gagaaggacg gcttcctttc cggcgccgcc gccctcgccc ggctcaccgg cccccgcgac
     3301 caggccgctc ccggcagccg tctcgccgag ctctccctgt ccgccctgcg ctacggccac
     3361 tccctgaccc tggag
//
LOCUS       DASPJI010000554         5488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_603229, whole genome shotgun sequence.
ACCESSION   DASPJI010000554 DASPJI010000000
VERSION     DASPJI010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603229"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="VEL74_15350"
     CDS             <1..1340
                     /locus_tag="VEL74_15350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HYG63950.1"
                     /translation="PIRFLFPVYRGARVPGWQIDLGLWMYGRMTGGRQKHQRLGPDEV
                     ERLAPGIVRDGLDTTFAYGDARADDARLTLAVAATGFAYGGLLLTRAQVEEPVRDAGG
                     RLRGLRVRDLETGEVHGVEARLVINATGVWVDEVRHRLGLEGRRVRPSRGSHLILSRD
                     RLPLTAALTVPSPDDGRPVFFIPHPEGVLVGTTDLFHEEGLDDPRPTRQEVDYLLRTL
                     QAVFPGRGITAGDVLGAFAGVRPIVDTAADTPSAASREEAIWEEQGLLSIAGGKLTTW
                     RAMAEEVVDRALTLLPAGRAAAAAPCATAGTPVGALAPPDLVRRLRSVHGLAPDVASG
                     MARRLGSLAWTACQEAGAAGDLRPLVDGADLTAAEARAHLRFGAVTRLEDLLLRRARF
                     GMWAPAMARELVPRLAPLIRRELGWDLARWEQETETAAAALTAWSPEGVREAS"
     gene            1337..2248
                     /locus_tag="VEL74_15355"
     CDS             1337..2248
                     /locus_tag="VEL74_15355"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HYG63951.1"
                     /translation="MKMKGTVFLVNPRAGSGRAGRVWEELRAALPDLAAANVVTAPDP
                     EEAARALDNRLREDAGPPVSRVIVVGGDGSVHLALNRIFAAGRQGDVALGLVPAGTGS
                     DLARTLGLPANPRTALVRVLAAPSRPVDVLEAATAAERRWVLNIASAGISGLVDALVN
                     ERPVRNATTFLVSTLQAMGRYTPITCRVELDGEPWYEGGIFVLAVANGRSFGRGMRIA
                     PRAELDDGLADVVLVRPIPRWQVPLRIHRLYLGRHLEQPHVLWRRARRVRLEPAGPIP
                     PFDVDGELMPSGAASFELLPKALRFLM"
     gene            2292..4034
                     /locus_tag="VEL74_15360"
     CDS             2292..4034
                     /locus_tag="VEL74_15360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG63952.1"
                     /translation="MIRPTLSALLSIILVAAPAVSQQPRPQQPVHGAGDVFSEVIEVR
                     AVNVQVVVTDRRGNRVPGLPAEDFRILLDGKEVPLDYFAEVREGRIAMPAGAGQSEKA
                     LAGLPPGLHDVGAGEVVGTNYLIFIDELFSPVHLRSEALKVMAGGADKLREQDRMAIV
                     AFNGRDLRVLSDWASPATLKELFRKLAREKRSLTGIGPPTNPLAAAKGPWLEPLGAMP
                     GQDVPDPANERDVEAQATRQKDGSSLVSPDLDVMEAGLEVDSVKHVVAAATATVRGFS
                     ASTPPGRRVMLLLSGGWSFNPASFYANADFELAKRMLQVSWDSGYRVLRPLIDASNLL
                     GYTIYPVHLAEPGSMLQSAEMRQSGAYESMFLMDQHLHSQSSLASVAVETGGRLLVPG
                     ARHLQRVEEDTSSYYWLGFSQSGGDDKRRNLKVEVRRKGLNARARSSFLPLSRSARAS
                     IDVETALTAGGVPNAGPLNVDVGTPRRSGLAAMELPVSVRIPVEQITLLQEGGRHLAR
                     LELRVASLDRGGRRSEIPVLPIVISKDQPPKRGMVVVYDVRLKLRRDAQDLQLVLHDL
                     YGGTDLAARVRVVP"
     gene            4078..4848
                     /locus_tag="VEL74_15365"
     CDS             4078..4848
                     /locus_tag="VEL74_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYG63953.1"
                     /translation="MLEVLARQFLYRPPALPVPSPPPPPLEEVWLDLATGDRAMVWTS
                     VDPMPPGAPVVLFFHGNSENLETMRMAGLCQRLEGVGVAFLAVDYPGYGRSSGEPSEE
                     GLLATGAAAVDWARRRQPGRPVVACGWSMGAAVAIATAAAHPGKVDGLVALCPWTRLE
                     DVVRRYAPELPAVFPVAHRYDSLSAARKVRVPALVIHGELDPVIPVEQGRQIARALGG
                     PSRFIPIPNARHRDLLVSHPQVWRELSGFFASLRAEPS"
     gene            complement(4861..>5488)
                     /locus_tag="VEL74_15370"
     CDS             complement(4861..>5488)
                     /locus_tag="VEL74_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HYG63954.1"
                     /translation="AVPSSPRAAAPPAYDPAPAPRPGSSYDYAPSAGSRSRFPGAGGL
                     AILAGMLGLGGWTAYRYRSRKCPSCKNPMIRLGEVQDDAHLTPPEATEERIGSVDYDV
                     WACASCDHVAKLRYGRLFSGYSECPACQTKALSSRSTTVQAATYTHGGLVRIDRDCAH
                     CGHKDSSTRSTPRRVQQRRTSSFSSGGGSRSGGFGGGRSSGRGASGRW"
BASE COUNT          671 a         2023 c         2013 g          781 t
ORIGIN      
        1 agccgatccg cttcctcttc ccggtctatc gcggcgcccg cgtcccgggc tggcagatcg
       61 acctcgggct ctggatgtac ggccgcatga ccggcggccg gcagaagcac cagcgcctcg
      121 gccccgacga ggtggagcgg ctagctcccg gcatcgtccg tgacggcctc gacaccacct
      181 tcgcgtacgg cgacgcccgg gccgacgacg cccgcctcac cctggccgtg gccgccaccg
      241 gcttcgccta cggggggctc ctcctcaccc gcgcccaggt cgaggagccg gtgcgcgatg
      301 ccggcggacg cctgcgcggc ctgcgtgtgc gcgacctcga gaccggcgag gtgcacgggg
      361 tggaggcgcg gctggtgatc aacgccaccg gcgtctgggt ggacgaggtg cgccaccgcc
      421 tcggcctcga ggggcggcgg gtgcgcccgt cccgggggag ccacctgatc ctgtcgcggg
      481 accggctgcc gctaaccgcc gctttgaccg tcccctcgcc cgacgacggc cggcccgtct
      541 tcttcatccc gcacccggaa ggggtgctcg tcggcaccac cgacctgttc cacgaggagg
      601 ggctggacga cccgcgcccc acccgccagg aggtggacta cctcctgcgc accctgcagg
      661 cggtcttccc cggccggggg atcacggccg gggacgtcct cggcgccttc gccggcgtgc
      721 gcccgatcgt cgacaccgcg gccgacaccc cgtccgccgc cagccgcgag gaggcgatct
      781 gggaggagca ggggctgctg tccatcgccg gcggcaagct caccacctgg cgggccatgg
      841 ccgaggaggt ggtcgaccgc gccctgacgc tgctccctgc cgggcgggcg gccgccgcgg
      901 cgccgtgcgc caccgccggc acccccgtcg gggccctcgc gccgccggac ctcgtccgcc
      961 ggctccggtc cgtccacggc ctcgcgccgg atgtggcgtc cggcatggcg cgccgcctcg
     1021 gctccctcgc ctggaccgcc tgccaggagg ccggcgccgc cggggacctc cggccgctgg
     1081 tggacggcgc cgacctcacc gccgccgagg cgcgggcgca cctccgtttc ggtgccgtga
     1141 cccgcctcga ggacctgctg ctgcgccgcg cccgcttcgg catgtgggcc ccggccatgg
     1201 cccgcgagct ggtgccgcgc ctggcgccgc tgatccgccg ggagctgggg tgggacctgg
     1261 cgcgctggga acaggagacc gagaccgcgg cggcggccct caccgcctgg tcgcccgagg
     1321 gggtgcgaga ggcgtcatga agatgaaggg cacggtcttc ctggtgaacc cccgcgcggg
     1381 gagcggccgg gccggacggg tctgggagga gctgcgcgcc gccctgccgg accttgcggc
     1441 ggccaacgtg gtgaccgcgc ccgaccccga ggaggcggcc cgggctctgg acaaccggct
     1501 gcgggaggac gccggccctc cggtctcgcg cgtcatcgtg gtggggggcg acggctccgt
     1561 gcacctcgcc ctcaaccgca tcttcgccgc ggggcggcag ggggacgtgg cgctggggct
     1621 cgtcccggcg ggcaccggct ccgacctcgc ccgtaccctg ggcctgccgg cgaatccccg
     1681 gaccgcgctg gtgcgggtgc tggccgcgcc gtcccggccg gtggacgtgc tggaggccgc
     1741 caccgcggcc gagcgccgct gggtgctcaa catcgcctcc gccggcatct ccggactggt
     1801 cgacgccctg gtcaacgagc ggccggtgcg caacgccacg accttcctgg tgtcgaccct
     1861 ccaggcgatg ggccgctaca cccccatcac ctgccgggtc gagctggacg gcgagccctg
     1921 gtacgagggg gggatcttcg tcctggccgt ggccaacggc cgctccttcg gccgggggat
     1981 gcgcatcgcg ccgcgggccg agctggacga cggcctggcc gacgtggtcc tggtgcgccc
     2041 gatcccgcgc tggcaggtcc cgctccgcat ccaccggctc tacctcgggc gccatctgga
     2101 gcagccccac gtcctctggc ggcgcgcccg gcgggtgcgc ctggagccgg cgggtccgat
     2161 cccgcccttc gatgtggacg gcgagctgat gccctccggc gccgcctcgt tcgagctgct
     2221 gccgaaagcg cttcgcttcc tgatgtgaca gggttacact aggtctttga tacaaaggcc
     2281 tggaggtctc cgtgatccga cccacgctct ccgcccttct ctcgatcatt ctcgtcgccg
     2341 ctcccgccgt ctcccagcag ccccgtccgc agcagcccgt gcacggcgcg ggggacgtct
     2401 tctcggaagt catcgaagtg cgggccgtca atgtgcaggt ggtcgtcacg gaccggcggg
     2461 gcaaccgggt ccccgggctt ccggcggagg atttccgcat cctgctggac ggcaaggagg
     2521 tcccgctgga ctacttcgcc gaggtccgcg aggggcggat cgcgatgccg gcgggtgcgg
     2581 gccagagcga gaaggcgttg gccggcctgc ctcccgggct gcacgacgtc ggagccggtg
     2641 aggtggtggg gaccaactac ctgatcttca ttgatgagct gttctccccg gtccacctgc
     2701 gctccgaagc cctgaaggtg atggccgggg gcgccgacaa gctccgcgag caggaccgga
     2761 tggcgatcgt ggccttcaac gggcgcgacc tgcgggtgct gagcgactgg gcctcgccgg
     2821 ccaccctcaa ggagctgttc cgcaagctgg cgcgggagaa gagatcgctg accgggatcg
     2881 ggccaccgac caatccgctc gccgcggcca agggcccctg gctggagccg ctgggggcga
     2941 tgccggggca agatgtgccg gacccggcga acgagcggga cgtcgaggcc caggccaccc
     3001 ggcagaagga tggctcctcc ctggtgagcc ccgacctgga cgtcatggag gcgggcctgg
     3061 aggtggattc ggtcaagcac gtggtcgccg cggccaccgc caccgtccgg ggcttctccg
     3121 cctcgacgcc gccgggccgc cgggtcatgc tgctcctctc gggcggctgg agcttcaacc
     3181 cggcgagctt ctacgccaac gccgacttcg agctggcgaa gcggatgctg caggtctcct
     3241 gggacagcgg ctatcgcgtc ctgcgcccgc tcatcgacgc cagcaacctg ttggggtaca
     3301 ccatctaccc cgtccatctg gcggagccgg gctccatgtt gcagtccgcc gagatgcgcc
     3361 agagcggagc ctacgagtcg atgttcctga tggaccagca tctccactcc cagagctccc
     3421 tcgcctcggt ggcggtggag accgggggcc gcctgctcgt gccgggggcg cgccacctgc
     3481 agcgggtgga ggaggacacc agctcctact actggctcgg cttcagccag tcgggcggcg
     3541 acgacaagcg gcggaacctc aaggtcgagg tgcggcgcaa ggggctcaac gcccgggcgc
     3601 ggtcgagctt cctgccgctg tcgaggtcgg cccgggcgtc gatcgacgtg gagacggcgc
     3661 tgaccgccgg cggcgtgccg aacgccggcc ccctgaacgt ggacgtgggc accccccgcc
     3721 gctcgggcct ggcggccatg gagctgccgg tctcggtccg gatccccgtc gagcagatca
     3781 cgctgctcca ggaagggggg cggcatctgg cgcggctcga gctgcgagtc gcctcgttgg
     3841 accgcggcgg ccggcgctcc gagatcccgg tgctccccat cgtcatctcc aaggaccagc
     3901 cgccgaagcg cgggatggtg gtggtctacg acgtccggct caagctccgg cgcgacgccc
     3961 aggacctgca gctcgtgctg cacgacctct acggcggcac cgacctcgcc gcccgcgtgc
     4021 gcgtcgtacc ctaggccggt gcaagccggc aaggagcttg cttcgctctg acgggccatg
     4081 ttggaagtcc tggcccgtca gttcctctac cgtccccccg ccctcccggt cccctcgccg
     4141 cctccgccgc cgctggagga ggtctggctc gaccttgcca ccggggaccg cgccatggtg
     4201 tggacctccg tcgatcccat gccgcccgga gcgccggtgg tcctcttctt ccacggcaac
     4261 tccgagaacc tggagaccat gcggatggcc gggctctgcc agcggctgga gggggtgggc
     4321 gtcgccttcc tggcggtgga ctaccctggc tacggccgca gctccggcga gccctccgag
     4381 gaggggctgc tcgccacggg cgcggcggcg gtggattggg cgcggcgccg ccagcccggc
     4441 cggccggtgg tcgcctgcgg ctggtcgatg ggagccgcgg tggccatcgc caccgcggcg
     4501 gcccaccctg ggaaggtcga cggcctcgtg gctctctgtc cctggacacg gctggaggac
     4561 gtggtgcggc gctacgcgcc ggagctgccg gcggtctttc cggtggcgca ccgctacgat
     4621 tccttgtcgg cggcgcggaa ggtccgcgtt cccgcgctgg tgatccacgg cgagctcgat
     4681 ccggtgatcc cggtggagca ggggaggcag atcgcccgcg ccctaggagg cccgagccgc
     4741 ttcatcccca tccccaacgc ccgccaccgg gaccttctgg tctcgcaccc gcaggtctgg
     4801 cgagagctgt ccgggttctt cgccagcctg cgggccgagc cttcctgaag ggttccttct
     4861 tcaccagcgg ccgctggcgc cgcgtccgga ggagcggccg ccgccgaagc ctccggacct
     4921 ggagccgcca cccgatgaga aagaagaggt ccggcgctgc tggacccgcc gcggggtcga
     4981 gcgggtggag gagtccttgt ggccgcagtg ggcgcagtcg cggtcgatgc gcacgaggcc
     5041 tccatgcgta tacgtagccg cctgcaccgt cgtggaccgc gagctcaggg ccttggtctg
     5101 gcaggccggg cactccgagt agccggagaa gagcctgccg tagcgcagct tggccacgtg
     5161 atcacaactc gcgcaggccc agacgtcgta gtcgacgctg ccgatccgct cctcggtggc
     5221 ctcgggcggc gtgaggtggg cgtcgtcctg gacctccccc agacggatca tcgggttctt
     5281 gcagctcggg catttccgcg agcgatagcg gtaggccgtc cagccgccca ggccgagcat
     5341 gccggccagg atggcgaggc ctccggcccc gggaaagcgg gagcgggagc ccgcggaggg
     5401 ggcgtagtcg tagctcgacc cgggccgcgg cgcgggagcc ggatcgtaag ccgggggggc
     5461 cgccgcccgt ggcgaggacg gcaccgcc
//
LOCUS       DASPJI010000555         9905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_148124, whole genome shotgun sequence.
ACCESSION   DASPJI010000555 DASPJI010000000
VERSION     DASPJI010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9905)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9905
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148124"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2463)
                     /locus_tag="VEL74_15375"
     CDS             complement(<1..2463)
                     /locus_tag="VEL74_15375"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63955.1"
                     /translation="MPSTLQRRVHPAFSVLVTLLLLAGLSPLQAAEGRWSPIGPEGGS
                     VRAFGVSPSDHTVYAGTSTGMLFRSTDAGASWTMVSKGAAAGFRDILVHPSNPRILYT
                     RADYLEDEPFHFPGHFPGAALLAKSTDAGETWTSIGPDTTVLSLVMAPSAPDTLYAGE
                     SGKVIRSTDGGATWTPTTLSQRAGALAVDPVAPGTVYAGAEQGLYKSLDGGATWALLS
                     GGATGSAPRGALTLTIDPSRPATVYAGDYGGIWKSLDGGTTWAPAHGGIEPDNKTEVV
                     ALAIDPSATSTLYASVRRMRDHYWGTGEIWKSTNGGSAWVQVHSTSELNQFIHELALP
                     PASPNRVLAGVETLAVLASGDGGQTWAPANRGLTSHVVWDLESSPFPDDTLLAATSLG
                     LFRSTDRGAHWERVLTTRTPYLAAFHPRQAGVVYAGAIDGLYRSTDFGRNWTLLTATG
                     APLFTVGSFVIDPSEPRNLYATGYRLQGNVPINGIARSTDGGASWIDMGISGAGRVLT
                     FSIDPVRPSRLYASGTGGVFRSTDRGKTWQQLTEGLPSRLLEILVDPDRPDTLYAVAK
                     STADLYRSDDGGDHWHPVSSTGLAVEPFTFVNKLAKAEFSNRLYATTMWGVYLSTDGG
                     QTWEPWSDGLEGLSVGDILIDPTNPYRLYAASGGGSVLERSLPLPDCVTGAESLCLNG
                     GRFLAEVTWRLPDGTSGRGQASPLAGETGGFWFFNESNLELMIKVLDGRAVNESFWVF
                     YAALSDVEYTVRITDTVTGETRLYRNPAGRMASVADTSAFRAGEAAAASARPAAQAAA
                     TAQDCGLTSGESALCLQDARFTV"
     gene            2672..2863
                     /locus_tag="VEL74_15380"
     CDS             2672..2863
                     /locus_tag="VEL74_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HYG63956.1"
                     /translation="MKRKIVGFHQDERDDWVADLECGHTQHVRHDPPWMNRPWVVTPE
                     GRARFLGFELDCKKCDEPG"
     gene            complement(2870..3235)
                     /locus_tag="VEL74_15385"
     CDS             complement(2870..3235)
                     /locus_tag="VEL74_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HYG63957.1"
                     /translation="MPSKDALYDLLRSLPGVTEDVKWGNDLVFSVGDKMFAVFMLPDG
                     EPLSFKVDEAAFASLTQQPGITPAPYLARHSWVKLESRRTLPAAALEEMLRESHRLVA
                     AKLPKKTRAALGLTEPPPA"
     gene            complement(3263..3649)
                     /locus_tag="VEL74_15390"
     CDS             complement(3263..3649)
                     /locus_tag="VEL74_15390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG63958.1"
                     /translation="MSQKILIADDDRVFVELLATRLHREGFEVSVVFDSVQAMMTAMR
                     LRPDVLLLDVKMPGGTGLDALRKLKRSWLTAAIPVIVASTLDNPELPATVQALGAAEF
                     LHKPVPYEVVKGSLTRALGSGGRAPV"
     gene            complement(3659..4441)
                     /locus_tag="VEL74_15395"
     CDS             complement(3659..4441)
                     /locus_tag="VEL74_15395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG63959.1"
                     /translation="MLNERLLEPQAPAEPALPPLRILLAEDSRLAEVYTVRLLERRGC
                     SVVVARNGREAVAACHRQPFDLILMDVQMPEMNGIEATAAVRASEREHGRRTPILALT
                     AGTTPEENESCRIAGMDGCLLKPVREQDLSAAFAGLAPVAADGGREESPSLDRAALMA
                     EAGGDGELLERLVAIFREEAARLLAEIRQAAVRGDGTRLSDAAHALKGTAGHWSRGAV
                     FRAAARLENRELGVDPEAAAADIKELQREIPRLLLALDHILS"
     gene            complement(4660..5193)
                     /locus_tag="VEL74_15400"
     CDS             complement(4660..5193)
                     /locus_tag="VEL74_15400"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYG63960.1"
                     /translation="MVGLNPLWSSIILTTRLAPANSNFFLDYDEAEVQRMFAEFPALA
                     PRRHPNTAQRIDALWLVVADLDAAIDFYEGLGLEVRFNHKEVHYLGARGAEVRYENAT
                     LALLVPDGPGPVADFAAARGEGIMGASIEVGDLDTAHSLVTGNTGLNLPTFQYKGRDR
                     LLIPASLPHGFLIEMVE"
     gene            complement(6978..8942)
                     /locus_tag="VEL74_15405"
     CDS             complement(6978..8942)
                     /locus_tag="VEL74_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYG63961.1"
                     /translation="MIAVSDLSKSFGPQTLFEGVSFQFNPGNRYGLVGANGSGKSTFL
                     KMLAGEEPASSGTIAIPKRLKLGVLKQDHYRYEHMPILEVTMMGDHEVWEAMVEKERL
                     LADADKEFDADRYADLEDLILRHDGYTLESRAGEVLEGLGIPTEVHRLPMSTLSGGFK
                     LRVLLAQVLASEPDVLLLDEPTNHLDILSIRWLEKFLVDSYKGTAVVISHDHRFLDNI
                     CTHIVDVDYETMILYPGNYTAFTEAKVGNRDRKEAEIEKREAEIARHKEFIDRFKAKA
                     TKARQAQSKVKMMEKIVIERLPQTSRRYPTFKFKQGRPSGRQVLAVEGISKSYGDKQV
                     LKDVSLTVERGDRLAIIGPNGIGKSTLLKIAMGEVEADAGKIEWGYEAWRGYFSQDHH
                     ELPKGSKQTVEAWLWEAVPGEVIGFVRGNLGMVLFSGDDVKKPIGSLSGGEAARLVFC
                     KLSVTKPNVLILDEPTNHLDLEAIEALVEALKEYDGTLIFVSHDRWFVSQLADRILEI
                     SPKGIQDFRGTYEEYLDRLGDDHLDAEAVLRMRREQKKAAALAAGNAAGDAAGNGKGN
                     GSGLDDRQRQQRQKELGKRRDQVTATVEQAESRINAINEMFCDPTFFDRTSRDKVKKL
                     EDEQKKLSTQVGELMGEWEKIEEELTQLGT"
     gene            9212..9448
                     /locus_tag="VEL74_15410"
     CDS             9212..9448
                     /locus_tag="VEL74_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HYG63962.1"
                     /translation="MKKPMTPTELRKDLYRVLDDVLETGVPQEVIRGARMLMIVPAGG
                     RRLQLDDLTRREALACSPEELVATSWEQEWSPES"
     gene            9445..9822
                     /locus_tag="VEL74_15415"
     CDS             9445..9822
                     /locus_tag="VEL74_15415"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYG63963.1"
                     /translation="MRAFLDTHAAIFLYEGRAELFGAASRELLERAALHVSPMVRLEM
                     GFLKEVGKLKVDPDLLLGTLAADYGVTATSDTLDSLVPLAMPLTWTRDPFDRLLVATA
                     LLHQAPLVTRDARIQECFDGAVW"
BASE COUNT         1668 a         3243 c         3344 g         1650 t
ORIGIN      
        1 cacggtgaaa cgggcatcct ggagacagag ggcgctctcc cccgaggtca ggccgcagtc
       61 ctgggcggtg gcggcagcct gtgccgccgg tctcgcggag gccgccgcgg cctctccggc
      121 acggaacgcc gaggtgtccg cgacgctcgc catccggcca gccgggttgc gatagagccg
      181 cgtctccccc gtaaccgtgt cggtgatcct cacggtgtac tcgacgtccg agagcgccgc
      241 gtagaagacc cagaagctct cattgacggc acggccgtcc agcaccttga tcatcagctc
      301 cagattgctc tcattgaaaa accagaagcc cccggtctct ccggccaatg gagaggcctg
      361 ccccctgccg ctggtgccgt cgggcaagcg ccaggtcacc tcggcgagga agcgcccacc
      421 attgaggcac agggactcgg cgccggtgac gcagtcaggc agcggcaggc tcctctcgag
      481 cacactgcca cccccgctgg ccgcgtagag gcgataggga ttcgtcggat cgatcaggat
      541 gtcgccgacc gacaaccctt ccaacccgtc gctccagggt tcccaggtct gaccaccgtc
      601 ggtgctcagg tagacgcccc acatggtggt tgcgtagagc cggttggaaa actcggcctt
      661 ggccagcttg ttgacgaaag tgaagggttc caccgccagg ccggtgctgc tcactgggtg
      721 ccaatgatcg ccgccgtcgt cgctccggta cagatccgcg gtcgacttgg cgaccgcata
      781 cagagtgtcg gggcggtcgg gatcgacgag gatctcgagc agccgggagg gtagcccctc
      841 ggtcagttgc tgccaggtct ttccccggtc cgtgctcctg aacaccccgc cggttccgct
      901 ggcgtagaga cgagaggggc gtaccgggtc gatgctgaag gtgaggacgc ggccggcgcc
      961 gctgattccc atgtcgatcc aggaagcgcc gccgtcggtg ctgcgcgcta ttccgttgat
     1021 gggaacattg ccctggaggc gatatcccgt ggcatagagg ttcctgggct ccgagggatc
     1081 gatgacaaaa gaaccgacgg tgaagagcgg cgcgccggtg gccgtgagca gagtccaatt
     1141 ccggccgaaa tccgtgctcc tgtagaggcc atcgatcgca ccggcataga cgacacccgc
     1201 ctggcgggga tggaaggccg ccagataggg agtccgtgtg gtcagaaccc tttcccaatg
     1261 cgcgccgcga tcggtgctgc ggaagaggcc gagggaagtg gcggcgagca gggtgtcatc
     1321 cggaaagggg gacgactcca gatcccagac aacatgagag gtaaggcccc ggttcgcggg
     1381 tgcccaggtc tgtccgccat ccccggaagc gaggaccgcc agcgtctcca ccccggcgag
     1441 cacacggttc ggcgacgctg ggggcagcgc cagctcatgg atgaactggt tgagctcgct
     1501 cgtcgaatgg acctggaccc atgccgatcc gccattggtg ctcttccaga tctctccggt
     1561 tccccagtag tgatcccgca tccgccggac ggaggcatac aacgtggacg tcgcggatgg
     1621 atcgatggcc agcgccacca cctcggtctt gttgtccggt tcgatcccgc cgtgggccgg
     1681 agcccaggtc gtcccgccat cgaggctctt ccagattcca ccgtaatcgc cggcatagac
     1741 ggtcgctgga cggctcgggt cgatcgtcag agtcagggct ccacggggag ccgatccggt
     1801 ggctcccccg ctcaggagag cccaggtcgc gcctccgtcg aggctcttgt agagcccctg
     1861 ctcggcgccc gcgtagacgg tgccgggagc gaccgggtcg acggccaggg cgccggctct
     1921 ctggctcaga gtggtgggcg tccaggtcgc accgccgtcg gtgctgcgga tcaccttgcc
     1981 gctttccccg gcatagagcg tgtccggcgc ggagggggcc atgaccagag agaggacggt
     2041 cgtatccggg ccgatcgagg tccacgtctc tcccgcgtcg gtgctcttgg ccagaagagc
     2101 ggcgccaggg aagtggccag ggaagtggaa cggctcgtcc tccaggtaat cggcccgcgt
     2161 gtagaggatc cgaggattcg acgggtggac caggatgtca cggaaaccgg ctgcggcccc
     2221 cttgctgacc atcgtccacg acgcgcccgc gtcggtgctg cggaagagca ttcctgtcga
     2281 agtcccggcg tagacggtgt ggtccgaggg agaaaccccg aacgcccgca cggaaccgcc
     2341 ctcggggccg atcgggctcc agcgcccctc ggccgcctgc agaggcgaga ggccggcgag
     2401 caggaggagg gtgacgagca cggaaaaggc cgggtggacg cgacgctgga gggtcgatgg
     2461 catatccatt cctcgaggaa gctcaggggt ggctcgacga aagaggcctc tggcaccaga
     2521 gggcctctct gcccctatta tgaacggtcg gggaattctg tttcgcaccc cgtcccactc
     2581 tcgacgatac tccttccacc cggcggatgg cggtccgact cccggcgccc ccgcttcgtg
     2641 ctaccttgac gctggaggcg ggctcccgag cgtgaagaga aagatcgtcg gcttccatca
     2701 ggacgaacgg gacgactggg tcgcggacct ggagtgcggg cacacccagc acgtccggca
     2761 cgacccgccc tggatgaacc ggccctgggt cgtcactccc gaagggcgtg cgaggttcct
     2821 cggcttcgag ctggattgca agaagtgcga cgagcctgga tagcggcgct caggcgggcg
     2881 gcggctccgt gagcccgagc gcggcccgcg tcttcttcgg cagcttcgcg gcgacgagcc
     2941 gatgggactc ccgcagcatc tcctcgagcg ccgccgccgg cagggtgcgg cggctctcca
     3001 gcttcaccca cgagtgacgg gccaggtagg gcgccggcgt gatccccggc tgctgggtga
     3061 gcgaggcgaa ggcggcctcg tccaccttga agctgagcgg ctcgccatcc ggcagcatga
     3121 agacggcgaa catcttgtcg cccaccgaga aaacgagatc gttgccccac ttgacgtctt
     3181 cggtaacgcc ggggaggctc ctcaggaggt cgtagagagc gtctttcgag ggcatgcgga
     3241 tcccatccag acgcccggta gtctacaccg gcgcgcgccc gcccgagccc agcgcgcgcg
     3301 tgaggctccc cttcacgacc tcgtagggca ccggcttgtg gaggaattcg gcggctccga
     3361 gcgcctggac ggtggcgggc agctccgggt tgtccagggt gctcgccacg atcaccggga
     3421 tcgccgccgt caaccaggac ctcttgagct tgcgcagggc gtcgaggccg gtcccgcccg
     3481 gcatcttgac gtccaacagc aggacatcgg gccgcaaccg catggcggtc atcatcgcct
     3541 ggacgctgtc gaacaccacc gaaacttcga agccttcacg gtgcagccga gtcgcgagca
     3601 gctcgacgaa aacacgatca tcatccgcga tcaggatttt ctgtgacatt ggagacctct
     3661 acgagagaat gtgatccagc gccaggagca ggcgggggat ctcgcgttgc aactctttga
     3721 tatccgctgc ggcggcctcc ggatccacgc cgagctcacg gttctccagc cgggcggctg
     3781 cccggaatac ggcgccccgc gaccagtgcc cggcggtccc cttgagcgca tgcgcggcgt
     3841 cggacagcct cgttccatca ccgcgcacag ccgcctgccg gatctccgcg agcaggcgcg
     3901 ccgcctcctc acgaaaaatc gcgaccagcc tttccaggag ctcgccatcg ccgccggcct
     3961 cggccatcag ggcggcccga tcgagggaag gagactcctc gcgcccgccg tccgccgcca
     4021 ccggcgccag cccggcgaag gcggccgaca ggtcctgctc ccggaccggc ttgagaaggc
     4081 agccatccat accggcgatc cggcagcttt cgttctcctc cggcgtcgtc cccgcggtca
     4141 gggccaggat gggggtgcgc cggccgtgct cccgctccga cgcacggacg gcggccgtcg
     4201 cttcgatgcc gttcatctcc ggcatctgca catccatcag gatcagatca aaaggctggc
     4261 ggtgacaggc ggctacggct tcgcgcccat tgcgggccac gacgacggag cagccgcgcc
     4321 gctcgagcag gcgcaccgtg tagacttcgg cgaggcggct gtcctcggcg agcaggatgc
     4381 ggagcggcgg aagcgccggc tcggccggcg cctggggttc gagcaggcgt tcgttcagca
     4441 cgcaaatcct ttctatggct aacgagccaa ggaaagcatc gaccgtgcca agccgttctc
     4501 ccccgagtca ccggaccgcc gcgtccagtc ttcggacgga tcgtccagga accggacgcc
     4561 ccagcattga ccagatcaga ggcgctcagg ccggaatccg gctcctgagc ctgagcgctt
     4621 gtcggggttt gtttggcagc ttccgggcga cgagcccgcc tactccacca tctcgatcag
     4681 gaagccgtgg ggcagggagg cggggatcag aaggcggtcc cggcctttgt attggaaggt
     4741 gggcaggttc aggccggtgt tgccggtgac caggctgtgc gcggtgtcga gatcgccgac
     4801 ttcgatgctc gcgcccatga tcccttcgcc gcgcgcggcc gcgaagtccg ccaccgggcc
     4861 gggaccatcc ggcaccagca gcgccagggt ggcgttctcg taccgcacct ccgcgccgcg
     4921 agcgcccagg tagtgaacct ccttgtgatt gaaacggacc tcgaggccga gcccttcgta
     4981 gaaatcgatg gccgcgtcca gatccgccac caccaaccag agggcatcga ttctctgcgc
     5041 cgtattggga tgccgccggg gcgccagggc cgggaactcg gcaaacatcc gctgcacctc
     5101 ggcctcgtca tagtccagga agaaattcga gttggcggga gcgagccggg tggtgaggat
     5161 gatcgaactc cagaggggat tgagcccgac cacctcgccg ggccggcccc gccggtcaaa
     5221 gaaacgattg aatcaccatc ccggagtaaa tgcgatcgaa gcccgctcga cgtcgtcctc
     5281 cgcgtcgagc gggccctcac ctgcgccacc gaagagctga cgagacctct gcccatgtga
     5341 ccgtggggta cgaattcagg cgcagagcct cacggaatca catccacgca ctcgcacctc
     5401 ggaagaccgt ctgccgggca ggtatatgag aaagtttcat gacatcctcc ccatagtcca
     5461 tacctccctg ggaccaggca cgaggatccg gcactcatat tcgcgcaatt gagtgagctg
     5521 catgctccac acatataggc aaacgacgac ctttcagctc cgctgccatc gacagcccgt
     5581 acatcttgag atggcttaga agcaggcagc tctgcggcga cagcctggtt cagggcgcag
     5641 aggagctgcg tggaggagct tccgtccgtt gccaaagcgt cggccacgcc tattgtcggc
     5701 acattcacgg cggccccttc cggggcgtcc gcggaggaaa tacctgtcag actcatcaag
     5761 atcagaatga ggaacagtcc aggcttcata cgatctcctt tcttggtctc ttacacgtga
     5821 gttagacaat cctgcaagaa gaggaccagc gctgtcggag gatcagccca acagttcccg
     5881 aagccccact cctcgcttga tggtcatccc tggggataca ggcagagggc gagatcctgc
     5941 cggccgcacg gatagaggca cggctcgtta tctggctcaa gagaaaccgg ggaaacaagc
     6001 gccgggaaac aagcgccgca cccccccggc gatctcgaag cgagctcgcg cggcctagag
     6061 gccaggttat aggtatatca cgcgaatcag aggcgatccc tcggcgttcg aatttgtcag
     6121 tctagttgcg gatgcagcgg ggcggtcgcc ctgcgcggtg cggcgccatc ctcgacgaac
     6181 gcgcggcggc ggtcagcgag gccggggttc aggcggccgg gtaggacatc ttctagatgg
     6241 atggagccac cggcgggcag ggccggtggc tcttttttct gggtggggat tggacctccg
     6301 cctcccctat catcttccgt ccgggtggag gtcccctcag gggcgcggcc ttggacacgg
     6361 cggcgccccc aatgcattca gttgtcaagg aacggcacag ccgctccgat ccatcgagac
     6421 tcggatggga gctcacttct gtatacggat gagggggggc ggagcgttca ggaaaagttg
     6481 gaggaatccc gctcccaggt ggagagcact gactccagca caggggacac ccactccgcc
     6541 atgatggtct ttggggaaga gagaggagtc atcggatccg ggacggaggt cccgcggaaa
     6601 ggggggcaga cgattcgctc cgacatcgag ccggtctgac ccgagatcga caccgcgcga
     6661 cccgagatcg agaagcttcg acccacgggg cgagtcattc gccccacggg gcaggtcatg
     6721 atgaaggcgg ggcggatggt tcgccccgtg ggtcaagtcg ttcgacccgt gggtcagacc
     6781 gcgatgaagg cggggcggac cgttcgaccc gtggggcgaa tcactaccga gccggggcga
     6841 gtccctcgcc ccctgggtca ggtctctcgg gaaccggggc ggatcgtctg ccccgcaggg
     6901 cgaagggtgt ggatctcgga gcgagcggcg tagatccggc tcgtagtgat ccggagcacg
     6961 gtttgtgatc cggaacgcta cgtcccgagc tgcgtgagct cctcttcgat cttctcccac
     7021 tcgcccatga gctccccgac ctgggtggag agcttcttct gctcgtcttc gagcttcttc
     7081 accttgtcgc gcgaggtgcg gtcgaagaag gtggggtcgc agaacatctc gttgatggcg
     7141 ttgatgcgcg actcggcctg ctcgacggtg gcggtcacct ggtcgcggcg cttgcccagc
     7201 tccttctggc gctgctggcg ctggcggtcg tccaggccgg agccgtttcc cttgccgttg
     7261 ccagcagcgt cgccggccgc attgccggca gccagcgcgg cggccttctt ctgctcgcgc
     7321 cgcatgcgca gcaccgcctc ggcgtccagg tggtcgtcgc ccagacggtc caggtactcc
     7381 tcgtaggtgc cccggaagtc ctggatcccc ttgggcgaga tctccaggat gcggtcggcg
     7441 agctgggaga cgaaccagcg gtcgtgggaa acgaagatca gggtgccgtc gtactccttg
     7501 agcgcctcga ccagcgcctc gatggcctcg aggtccaggt ggttcgtcgg ctcgtcgagg
     7561 atcaggacgt tcggcttggt caccgacagc ttgcagaaca ccagccgcgc cgcctcgccg
     7621 cccgacaggc tgccgatcgg cttcttcacg tcgtcgcccg agaacagcac catgccgagg
     7681 ttgccgcgca cgaagccgat gacctcgccg gggacggcct cccagagcca ggcctcgacc
     7741 gtctgcttgc tccccttggg cagctcgtgg tggtcctgcg agaagtagcc gcgccaggct
     7801 tcgtagcccc actcgatctt gccggcgtcg gcctccacct cgcccatggc gatcttgagc
     7861 agcgtcgact tgccgatgcc gttcgggccg atgatcgcca ggcggtcgcc ccgctccacg
     7921 gtgagcgaga cgtccttgag cacctgcttg tcgccgtagg acttggagat cccctcgacg
     7981 gcgaggacct ggcggccgct cgggcgcccc tgcttgaact tgaaggtggg ataccgccgc
     8041 gaggtctggg gcaggcgctc gatgacgatc ttctccatca tcttgacctt gctctgcgcc
     8101 tgccgggcct tggtcgcctt ggccttgaag cggtcgatga actccttgtg gcgggcgatc
     8161 tccgcctccc gcttctcgat ctccgcctct ttgcggtccc ggttgcccac cttggcctcg
     8221 gtgaaggccg tgtagttgcc cgggtagagg atcatcgtct cgtagtccac gtcgacgatg
     8281 tgggtgcaga tgttgtcgag gaaccggtgg tcgtgcgaga tcaccaccgc cgtccccttg
     8341 taggagtcga cgaggaactt ctccagccag cggatggaga ggatgtccag atggttcgtc
     8401 ggctcgtcga ggagcaggac gtcgggctcg gaggcgagca cctgggcgag gagcacgcgc
     8461 agcttgaaac cgcccgagag ggtggacatc ggcagccggt gcacctcggt cgggatgcca
     8521 agcccctcca gcacctcgcc cgcccgcgac tccagggtgt agccgtcgtg acgcaggatc
     8581 aggtcctcga ggtcggcgta gcggtcggcg tcgaactcct tgtcggcgtc cgcgaggagg
     8641 cgctccttct ccaccatcgc ctcccacacc tcgtggtcgc ccatcatggt cacctcgagg
     8701 atgggcatgt gctcgtagcg gtagtgatcc tgcttcaaga cacccagctt caggcgcttg
     8761 gggatggcga tcgtccccga gctcgccggc tcctcaccgg ccagcatctt gaggaaggtg
     8821 gacttgcccg agccgttggc cccgacgagg ccatagcggt tgcccgggtt gaactggaac
     8881 gacacccctt cgaacagagt ctgggggccg aaggacttgc tgaggtcgga gacggcgatc
     8941 atagggtcga aaatgatacc agcggcggca ccgcggaggt gaatcggcag agattaccca
     9001 gggagcgcgg ggcgctctga ccgggagaac cggcgggggg aggggtggca ttcccgacct
     9061 cgctcttcac gtacaagtaa aaatgcaaga tttttcccac gctttccgtc ttctcccggt
     9121 tgaaaggaga gaagccggcc agggaggcgg ggtgcttccc ttggcgaaac gtacgtgata
     9181 tccatatgac gtacgccgag gagggccggt catgaagaag cctatgacac caacggagct
     9241 gcggaaggat ctgtaccggg tgctggacga cgtgctggag acgggagtgc cccaggaagt
     9301 gatccggggg gcgcggatgc tgatgatcgt gccggccggg gggcggcggc tccagctcga
     9361 cgacctgacg cggcgggagg cgctggcctg ctcgcccgag gagctggtgg ccacctcctg
     9421 ggagcaggag tggagccccg agtcatgagg gcgttcctcg acacccacgc cgccatcttc
     9481 ctctatgaag ggcgcgccga gctcttcggc gccgcctcgc gggagctgct ggagcgggcg
     9541 gcgctccacg tctcgcccat ggtgcggctg gagatgggct tcctgaagga ggtggggaag
     9601 ctcaaggtgg accccgacct cctcctcggc accctggccg ccgactacgg ggtcacggcc
     9661 acgagcgaca ctctggactc cctggtgccg ctcgccatgc cgctgacctg gacgcgcgac
     9721 cccttcgacc ggctcctggt ggcgaccgcc ctcctccacc aggcgcctct cgtcacccgc
     9781 gacgcgcgca tccaggagtg cttcgacggc gccgtctggt gagtgaggcg ctccgggccg
     9841 gaggctggtg ccagcctccg gccgaggagg gcctggggct cccggggcta ggcgtcggcc
     9901 agcgt
//
LOCUS       DASPJI010000556         5387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_548859, whole genome shotgun sequence.
ACCESSION   DASPJI010000556 DASPJI010000000
VERSION     DASPJI010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548859"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>5387
                     /locus_tag="VEL74_15420"
     CDS             <1..>5387
                     /locus_tag="VEL74_15420"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG63964.1"
                     /translation="ERFIPDRFGGRLYRTGDLVRQLADGSLEYLGRIDQQVKIRGFRI
                     EPGEIEAVLAAHPAVRQIVVAVREEAPGDRRLAAYVTCDPSGLPLDEQEARQVEEWQA
                     VYEKDVFGRLPDPTFNVLGWDSSYTREPIPDEEMREWLDGTVDRLLGGRLLGRLRPAG
                     NRVLEIGCGTGLVLFRVAPRCDLYLGTDFSAAALGYVRGVLASLPEPIPGVELRQGLA
                     DDPATLEPADGGRFDAVILNSVVQYFPSAEYLARVLESAVERTAPAGFVFVGDVRSLP
                     LLDAFHAAVELYRAADDLPRERLARRVAAAVAADPELVLAPAFFAALARRLPRVSHVE
                     IELRRGHRWNELTRFRYDVVLHVEHPGEGEQPAWLDWQEEGLTLPALRERLAREEPEL
                     LGLRRVPNARVAAELGALELLQDPAGPWTARELRRESGERAGGVDPEEVWALAGEVPY
                     DVAITWSDDPACFDVVLRFRDVRGVPAPMPGLAPTVTAEMTGSWRRWASDPLRRQAER
                     LLVPRLLELARERLPAYMVPADVVVLEALPLTVQGKVDHRALPAPERVRPELEEGFLA
                     PRTPEERELAALWADLLGLERVGVRDNFFALGGHSLLATQAVSRVRQAFGVDLPLRAL
                     FEEPTVAGLAARVRAALETGDRPALPPVEPVPRKLGDPLPLSFGQQRLWFLGRMETAY
                     HMAGAFRLEGDLRPAALESALGEVVRRHEVLRTVYREVNGGPAQVIQPASPMALPLVD
                     LASLPSETRDAEVRRLGREEARRPFDLTRDLMLRGLLVRLSGREHVALVTLHHVASDG
                     WSIAILGSELGALYGALIQGRPSPLPALQIQYADFAAWQRRWLEGPVLDAQLAWWREH
                     LAGAPPLLELPGDRPRPAVRTSRAGRRAFALSAFLDRRLRELAAGEGTTPFAVLLAAF
                     LALLARLTDRRDRDLLIGAPVAGRRQAETEGLIGFFVNTVVMRAGAGGDPPFRELLGR
                     VHRAALGALAHQDLPFERLVEELQPPRNLSYTPLFQVMFSLQNQPAVALDLPGVTLTP
                     LPLEPESRFDLELTLSEEPGGLTGTLDFSLDLFDASTAGRWVEAYLRVLEEGTARPGT
                     RLSGLPLLSATELQQVLEWGRGPSLEPVEPGGASTVLELFAAQVERAPEAPALVWNGE
                     TLTYGELDRRAGGVARGLREAGVEPGMVVGLRIERSAELVAGLLGIMKAGAVFLPLDP
                     AYPEERLAWMVADSRAVLVMGESAQRVLTPRPPLPSPTLPPPGEGENGSRSLAYLIYT
                     SGTTGLPKAVAVEHGSLVHTLRSVVGLFGFGPGDRMPAIASFSFDIFLFEALAPLLSG
                     GTSVLLPLRPTLDLDLLLAELAASTRLHAVPAVLRQVVDRVLALGLAGRFPLAEIYVG
                     GDAVPPGLLADAAAAFPGVPVRVLYGPTEGTILATSWPVLPGGSVPSAVLGRPLPGVG
                     VDLLDTDGLPVPLGVAGEVVLSGPGITRGYLGRPDLTAERYRPAPGGSRVYRTGDLAR
                     WRPGGLLEFLGRADAQVKVRGFRVEPGEIEARLMAHAGVHQAAVTVIESGGEKHLAAF
                     FVPEKEAGIVEADLRDHLRRGLPEHMVPSILVALDALPLTPNAKVDRDALAVLAPSRP
                     AASVTETPATPAQEILAGLWREALGLPAVGIHDDFFALGGHSLLATRLASRVREVFGV
                     ELPLRLFFEEPTIAGLASRLDKADTRALPPILPRDTAEPLPLSFAQQRLWFLDQMHPG
                     DSSYNLPVVLRLDGPLDPGCLESALTEVVRRHESLRTTFPARAGEPYQLIHPPALFAL
                     PLVDLAALPAP"
BASE COUNT          607 a         1939 c         2004 g          837 t
ORIGIN      
        1 ggagcggttc atcccggatc ggttcggcgg gcggctctat cgcacgggcg acctggtacg
       61 gcaactcgct gacgggagcc tggagtacct ggggcggatt gaccagcagg tgaagatccg
      121 tgggttccgg atcgagcccg gcgagatcga ggcggtgctg gcggcgcatc cggcggtccg
      181 gcagatcgtg gtggccgtgc gggaggaggc gcccggggac cgccgcttgg ccgcctacgt
      241 cacctgcgat ccctcgggtc tgccgctcga cgagcaggag gcccggcagg tggaggagtg
      301 gcaggcggtc tatgaaaagg acgtcttcgg ccggctgccc gatcccacct tcaacgttct
      361 gggctgggac agcagctaca cgcgcgagcc gatcccggac gaggagatgc gggagtggct
      421 cgacggcact gtcgaccgtc tcttgggcgg ccgcctgctc ggacggctgc ggccggcggg
      481 caaccgggtg ctggagatcg gctgcggcac cggcctcgtg ctgttccggg tggcgccccg
      541 ctgcgatctc tacctgggga ccgatttctc ggccgcggcc ctgggttacg tgcgcggcgt
      601 gctggcgagc ctccctgagc cgatccccgg ggtggagctg cgccaggggc tggcggacga
      661 tcccgcgacg ctggagccgg cggacggcgg gcgcttcgac gccgtgatcc tcaactcggt
      721 ggtgcagtac ttccccagcg ccgagtacct ggcgcgggtg ctggagtccg ccgtggagcg
      781 gactgctccc gcaggcttcg tcttcgtggg tgacgtgcgg agcctgccgc tcctagacgc
      841 cttccatgcc gcggtggagc tgtaccgcgc ggcagacgac ctgccgcggg agcggctggc
      901 gcggcgggtg gcggccgccg tggcggccga ccccgagctg gtgctcgccc cggctttctt
      961 cgcggcgctg gcccgccgcc tcccccgggt gagccacgtc gagatcgagc tgcggcgcgg
     1021 gcaccgctgg aacgagctga cccggttccg ctacgacgtg gtcctccacg tggagcaccc
     1081 cggggagggg gagcagcccg cctggctcga ttggcaggag gaggggctga ccctcccggc
     1141 gctccgcgag cgtctcgccc gagaggagcc agagctactg ggcctgcggc gggtgcccaa
     1201 cgcccgcgtc gccgccgagt tgggcgcgct ggagctcctc caggatcccg ccggcccctg
     1261 gaccgcgcgc gagctgcgcc gggagagcgg cgagagggcc ggcggcgtcg atcccgagga
     1321 ggtctgggcc ctcgctggcg aggtgccgta cgacgtggcc attacctggt cggacgatcc
     1381 agcctgcttc gacgtcgtgc tgcgctttcg ggacgtgaga ggcgtgccgg cccccatgcc
     1441 ggggctggcg cccaccgtga ccgcggagat gacgggctcc tggcgccggt gggccagcga
     1501 ccccctgcgc cgccaggcgg agcggctgct ggtgccgcgg ctgctcgagc tggcgcggga
     1561 gcgattgccg gcctacatgg tccccgccga cgtggtggtg ctggaggcgc tgcccctcac
     1621 cgtgcagggc aaggtggacc accgggccct accggcgccg gaacgggtgc gcccagagct
     1681 cgaagagggg ttcctcgccc cccgcacccc ggaggagagg gagctggcgg cgctctgggc
     1741 ggacctcctg ggactggagc gggtgggggt ccgggacaac ttcttcgccc tgggtggtca
     1801 ctccctcctg gcgacgcagg cggtctcgcg ggtgcgccag gcgttcggtg tcgacctgcc
     1861 gctccgcgcc ctgttcgagg agccgacggt cgccgggctc gcggcgcggg tgcgggcggc
     1921 cctggagacg ggggaccggc cggcgctgcc gccggtggag ccggtgcccc ggaagctcgg
     1981 agatcctctc cccctgtcgt tcggccagca gcggctctgg ttcctcggcc ggatggagac
     2041 cgcctatcac atggccgggg cgttccgttt ggagggggac ctccggccgg ccgcgctgga
     2101 atccgcgctg ggtgaggtgg tgcggcgcca cgaggtgctg cgcacggtct accgggaggt
     2161 gaacggcggg ccggcgcagg tgattcagcc ggcgtcgccc atggccctgc ccctggtgga
     2221 cctcgcgtcg ctacccagcg agacccggga cgcggaggtc cgccggctcg gccgcgagga
     2281 ggcccgccgg cccttcgatc tcacccggga cctgatgctg cgtgggctgc tcgtccggct
     2341 ctccgggcgc gagcacgtcg ccctggtgac cctccaccac gtcgccagcg acggctggtc
     2401 cattgccatc ctcggaagcg aattgggggc cctctatggc gcgctgatcc agggccggcc
     2461 ttcaccgcta cctgctctac agatccagta cgcggacttc gcggcctggc agcgccgctg
     2521 gctggagggg cccgtgctgg acgcccaact cgcctggtgg cgggagcacc tggcgggggc
     2581 gccaccgctc ctggagctgc ctggcgaccg gccacgtccg gcggtgcgca cctcgagggc
     2641 cgggcgccgc gccttcgccc tctccgcttt cctcgaccgg cggctgcgcg agctggcggc
     2701 gggggagggg accaccccct tcgccgtgct cctggccgcc ttcctcgccc tcctcgcccg
     2761 gctcacggac cgccgcgacc gcgaccttct gataggcgct cccgtggccg gacggcgtca
     2821 agccgagacc gagggcctca tcgggttctt cgtcaacacc gtggtgatgc gcgccggtgc
     2881 cggcggcgat ccgccgttcc gtgagctgct cggccgcgtc caccgggcgg cgctgggtgc
     2941 cctcgcccac caggacctcc cgttcgagcg tctggtcgag gagctgcagc cgccgcgcaa
     3001 cctgtcctat acgccgctct tccaggtgat gttctcgctc cagaaccagc cggccgtggc
     3061 gttggatctg cctggggtga cgctcacgcc gttgcccctg gagccggagt cgcgcttcga
     3121 cctggagctg accctctccg aggagccggg cgggcttacc ggcaccctcg atttttccct
     3181 ggacctcttc gacgcctcca ccgccggacg atgggtggag gcctaccttc gggtgctgga
     3241 ggaggggacg gcacggccgg ggacccggtt gtccggcctg ccgctcctct ccgcgacgga
     3301 gctgcagcag gtgctggagt ggggccgggg gccatcgttg gagccggtgg agccgggcgg
     3361 ggcatccacg gtcctggagc tgttcgcggc gcaggtggag agggcgcccg aggctccggc
     3421 cctggtctgg aacggcgaga ccctgaccta cggcgagctg gaccgccggg ccggcggcgt
     3481 ggcgcggggg ctgcgggagg ccggcgtcga gccggggatg gtggtggggc tccggatcga
     3541 gcggtcggcc gaactggtgg cggggctcct ggggatcatg aaggctggag cggtgttcct
     3601 cccgctggat cccgcgtatc ccgaggagcg gctggcgtgg atggtggcgg actcgagggc
     3661 ggtcttggtg atgggggagt ctgcccagag agtcctcacc ccccggcccc ctctcccatc
     3721 tcccaccctc cccccgccgg gagaggggga gaacggctca cggtccctcg cctaccttat
     3781 ctatacgtcg gggacgacgg ggctgcccaa ggcggtggcg gtggagcatg ggagcctcgt
     3841 ccacacgctg cgctcggtgg tggggctctt cggcttcggg cccggcgacc ggatgccggc
     3901 catcgcatcg ttctcgttcg acatcttcct gttcgaagcg ctggcgccgc tgctctcggg
     3961 tggcacctcc gtgcttctcc ctctgcggcc gacgctggac ctggacctcc tcctggcaga
     4021 gctcgcggcc tccacccggc tccatgccgt gccggcggtc ctgcgccagg tggtggaccg
     4081 ggtcctggcg ctggggctcg ccgggcgctt ccctctcgcg gagatctacg tcggcggcga
     4141 tgccgtcccc ccggggctcc tggccgacgc ggcggcggcg ttccccggcg tgccggtgcg
     4201 cgtcctctac ggtcccaccg aggggaccat cctcgccacg agctggccgg tgcttcctgg
     4261 agggtccgtg cccagcgctg tcctggggcg gccgctgccg ggcgtaggcg tggacctctt
     4321 ggatacagac ggcctgccgg tccccctcgg agtggcgggc gaggtcgtcc tctccggccc
     4381 cgggatcacc cgcggctatc tgggacgccc ggacctgacg gcggagagat atcgacctgc
     4441 accggggggg agccgcgtct accgtaccgg cgacctcgcc cgctggcggc ccggcggcct
     4501 cctggagttc ctgggccggg cggacgcgca ggtcaaggtc cggggattcc gggtggagcc
     4561 cggcgagatc gaggcgcgcc tcatggccca tgcgggggtg caccaagccg cggtgacggt
     4621 gatcgaatcc ggaggagaga agcacctcgc ggccttcttc gtccctgaga aagaagcagg
     4681 gattgtggag gccgacctgc gggaccatct ccgccgcggc ctgcccgagc acatggtccc
     4741 ctccatcctg gtggccctgg acgctctccc cctcacgccc aacgccaagg tggaccgcga
     4801 cgcgctggcg gtgctggccc cgtcccggcc cgccgcctcc gtcaccgaga ccccggcgac
     4861 tcccgcacag gagatcctgg ccggtctctg gagagaagct ctgggcctcc ccgcggtggg
     4921 catccacgac gacttcttcg ccctgggcgg ccactctctt ctggccacgc gcctggcctc
     4981 ccgggtccgg gaggtcttcg gggtcgagct gccgctccgt ctcttcttcg aggagccgac
     5041 catcgccggc ctggcgagcc gcctggacaa ggcggacacc cgcgcgctgc cgcccatcct
     5101 gccgcgggac acggccgagc ccctgccgct ctccttcgcg cagcagcgcc tctggttcct
     5161 ggatcagatg catccggggg acagctccta caacctcccg gtcgtcctgc ggctcgacgg
     5221 ccccctcgat cccggctgtc tggagtccgc gctcaccgag gtggtccgcc gccacgaatc
     5281 cttgcggacc accttcccgg cccgtgccgg agagccctat cagctcatcc atccgccggc
     5341 gctcttcgcc ctgccgctcg tcgacctcgc cgcgctcccc gcaccgg
//
LOCUS       DASPJI010000557         9724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_36273, whole genome shotgun sequence.
ACCESSION   DASPJI010000557 DASPJI010000000
VERSION     DASPJI010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9724)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36273"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="VEL74_15425"
     CDS             <1..1349
                     /locus_tag="VEL74_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63965.1"
                     /translation="SGRLVGIYRWLHKIYEIALRGADKRLVVEFEIAVPAADFLLYIR
                     NLHGVHLTRPNPPESYGVSSWQDVTAENYLELASLYQIEVPPPPPAVVSVLRSFQSQP
                     PVFQAELEVPEGYRVTAGTVQYLLGDASDNLVGYVGPARFSHPAAPTTAILGLLQTPA
                     PASGSGGTTTCPALTDPFTYPQVPFQPYQAAEDLGLAGAAGKIPAAMLSTATWFSATV
                     TLTCGLPAGSELMTAWQIRTYDALVAGYLGSLRRYEEEIRVRIGASSAADKRRIERRQ
                     LQQRACRILWGRRAPAAPPSGGAPAEAIDAHCRELFERAFSWEEMTYAFHHWPPPPEP
                     CRPDPCWPGQALIYTEADGLFESFLRARSARALVPVRPGFAAAVLFYLHFGLSWPGAP
                     LEAPAAQPDLAVLADLRTPPESCDGSSWRLEIPTSMLVLQAGSEFPTFPCTLEKTS"
     gene            1346..2020
                     /locus_tag="VEL74_15430"
     CDS             1346..2020
                     /locus_tag="VEL74_15430"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HYG63966.1"
                     /translation="MNPGPSLFSPFPQTQSLPIGVPVGTVVPYAGPVDEDPDRASVRR
                     LRLAEQGWLLCDGSEVSKTHWRELYELIQDTYGKAGSADSFVLPDYRGAFLRGVNGDR
                     SPALDPAAGQRTASGEGGTGTGNQVGSFQTAQFQEHEHQYTAGTTMSAFQAGGVNAVI
                     STIESTTTAVVCGQGSGDSTSCYGAETRPVNIYVNYLIKGVDHQLRLTVPMEIPRLDP
                     RLGLIR"
     gene            2132..3175
                     /locus_tag="VEL74_15435"
     CDS             2132..3175
                     /locus_tag="VEL74_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63967.1"
                     /translation="MSRRVVYPGLAIHAVLFLLALAAGAQPPVKNQNNIRVTFVNIGA
                     GLCTVIECPGLPAQTAPILYDCGSSGRGDTGLDRAGTVQFVRTILSRYGVAPRVVVSH
                     PDGDHYNYITSIMGNANASSIWLGAQTADYNITFQNWLMAQQQAGVAINVFAQGASNN
                     GQPVGGLACGAGLLPNSGTWLLTVNAGDDDNAKSMVLRLAYGQFQATLTGDATAESLT
                     SLMYNYRANVANGSIATTLLGAPHHGSITEGSNSFVWPQVFQPRIVVYSAGERYYHPR
                     CEALDVYDDAGTLRNAQNHPLQCGDEGAYWLLDQANAQYLTESNGSVWVDANGQGGVQ
                     VTCDAANVNCNLN"
     gene            3274..3705
                     /locus_tag="VEL74_15440"
     CDS             3274..3705
                     /locus_tag="VEL74_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63968.1"
                     /translation="MSTLRTATFAAALTVSVVLGGCEYNPTAPAEVKDPSPSANPSSC
                     EAGPYRIHLNGQCRVATGRRIVCEDESTTTPADKILGITFQLRDAATGAPVETRSVSS
                     GVIPREISFSGRAPGSYEVRHTVQARDCTFMATTYSGLEVF"
     gene            3815..4024
                     /locus_tag="VEL74_15445"
     CDS             3815..4024
                     /locus_tag="VEL74_15445"
                     /inference="COORDINATES: protein motif:HMM:NF038153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I lanthipeptide"
                     /protein_id="HYG63969.1"
                     /translation="MKRNVKRLTLNRETLRYLNPQQMTDAAGGDVSGRICPVTDPCFV
                     TYFNCPPPTTVSQQTCPTNCGQNYC"
     gene            5395..>9724
                     /locus_tag="VEL74_15450"
     CDS             5395..>9724
                     /locus_tag="VEL74_15450"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYG63970.1"
                     /translation="MLKNATIAEILESRATEQGERSAYVFLSYGETGVVEERLTYADL
                     DNRARAIGASLQAAGAGGERVALLLPPGPDFVASFFGCLYAGAVAVPSLPPRRRGADP
                     RLAAICRDARPRVALTTDGQLPALESAAAEIPELAAALRMAPAPLGTAGAADWRRPDL
                     GSEALAFLQYTSGSTSTPKGVMVSHGNLLHNEELIRRTFEQSSDSVVLGWLPLYHDMG
                     LIGTVLQPLYTGAVCYLMTPGSFLQRPARWLEAVSRYRATTSGGPNFAYELCVRKVGE
                     AEREGLDLSSWRVAFNGAEPVRAGTLRRFADAFAPCGFRAEAFRPCYGLAEATLLVSG
                     WRQEGETQVRALDAEALERHEVLGSGDATRSRELVSCGAAMQTMLAVDPESGEPCPPG
                     RVGEIWVAGPSVAQGYWQRPEETAATFAARLADGTGPFLRTGDLGFVSEGEVFLTGRL
                     KDLIILRGRNHYPQDLELTAERSHAALRAGGGAAFAADRSGEERLVIVHEVERHARFD
                     AEGEGTEEIAAAIRQRVAEEHEVSVAEVVLIRPETLPRTSSGKVRRSTCRDLYLQGGL
                     RVIGASHLSPAAVDLDPASEVQVGSPDWLRRAFAAAARIDPSRVDADLPLAASGLDSL
                     AAVELKQTVEEATGVSLPLADLLEGMTLRELDRRLAGSTERLPAGEGLAPFREGASPS
                     PASNQVASGEHPLSWNQRSLWFLHRLAPESSAYNIAGAARLEAASFEALGRAIQALVN
                     RHPMLRATFADLPGGPVQRLPETTEGSEAPLEVVDATGWSDAEVDARLRAEAYRPFDL
                     AAGPLLRTVLLRRGDEAFFAFAVHHVAADFWSMAVLARELGALVAGETLPPPAVLYTD
                     FAHWQERMLESSAGEWLWEHWRERLAGTPQLDLPTDRPRRPVQALRGGARTLPPSLER
                     AEAVQRLAATHGCTPFVALLAIWQAVLSRWSGQEEFLTGAPMAGRSAREWGEVVGYFV
                     NLVPLRADLSGDLGAHELMARTRRTVLDALAHQDFPFALLTERLQPERDPSRPPLVAA
                     MLTLEKAPAPELAALAAFAVGMPGVRLDLGGVGVLETLALAPPAAQLDLSLTAAELPG
                     GLALSLQWDADLFDAATIDRLTGHFERLLVEMAVADPRPVAELPLLSPAERHQMLAEW
                     NDTALPRSRGALLQDLFEAQAARTPEAPAVAFQGETLTYAELGARAGRLARHLRRLGC
                     GAESRVGVALERSLDLVVALLGVLEAGAAYVPLDPGYPRERLVLVLEDAAPQVLITET
                     GLRAALPAAAGTAVLCLDAEALTEGPLDRVAGDDRQLAYVLYTSGSTGRPKGVGVPHE
                     ALVNFLGSMRRAPGFSPGERLLAVTSLSFDIAALEIFLPLITGGCVELASRVEAADAA
                     LLADRLRASGAGVLQATPATWRMLLDSGWPGDPHLRALCGGEGLPRDLAESLAGRTGE
                     LWNLYGP"
BASE COUNT         1395 a         3528 c         3262 g         1539 t
ORIGIN      
        1 gctccggccg gctcgtcggg atctatcgct ggctgcacaa gatctacgag atcgccctgc
       61 ggggtgcgga caagcgcctg gtggtggaat tcgagatcgc cgtgcccgca gcggacttcc
      121 tgctttacat ccgcaacctg cacggcgtcc acctcacccg tcccaatccg cccgagtcct
      181 acggagtgag ctcctggcag gacgtgaccg cggagaacta cctggagctg gcgagccttt
      241 accagatcga ggttccgccg cctccaccgg cggtggtctc cgtcctccgg agcttccaat
      301 cccagccgcc ggtcttccag gcggagctgg aggttcccga gggctatcga gtgaccgcgg
      361 gcacggtcca gtaccttctc ggcgatgcgt ccgacaacct ggtcggctac gtcggcccgg
      421 cccggttctc gcaccccgcc gcacccacca cggcgatcct cggcctgctc cagacgccgg
      481 cgcccgcatc gggctccggc gggaccacga cctgcccggc gctcaccgat cccttcacct
      541 acccgcaggt gccgttccag ccgtaccagg ccgcggagga tctcgggctc gccggggcgg
      601 cggggaagat ccctgccgcc atgctctcca ccgcgacatg gttctcggcc acggtgacgc
      661 tgacctgcgg gctgccggcc ggcagcgagc tgatgaccgc ctggcagatc cggacctatg
      721 acgcgctcgt ggcaggctat ctcggctccc tgcggcgcta cgaggaggag atccgggtcc
      781 gtatcggggc gagctccgcc gcggacaagc gccggatcga gcggcggcag cttcagcagc
      841 gcgcctgccg gatcttgtgg ggccgccgcg cgcccgcggc tcctccgtcc ggcggcgctc
      901 ccgccgaggc catcgacgcg cactgccgtg agctgttcga gcgcgccttc tcctgggagg
      961 agatgaccta cgccttccac cactggccgc cgccgccgga gccctgccgt cccgacccct
     1021 gctggccggg ccaggccctg atctacaccg aggcggacgg cctgttcgaa agctttctgc
     1081 gcgcccgctc ggcccgggcc ctggtgccgg tgcggccggg attcgcggcg gccgtcctgt
     1141 tctacctgca cttcggcctg tcctggccgg gagccccgct ggaggctccg gccgcccagc
     1201 cggacctggc cgtgctcgcc gacctcagaa ccccgccgga gagctgcgac ggctcctcct
     1261 ggcgtctcga gatcccgacc tcgatgctcg tgcttcaggc gggcagcgag ttccccacgt
     1321 tcccctgtac tttggagaaa acctcatgaa tccaggcccc tcgctcttct ctccgttccc
     1381 ccagacccag agcctcccga tcggcgtccc ggtcggcacg gtggtgccgt atgccggacc
     1441 cgtggacgaa gaccccgaca gagcgtccgt ccggcggctg cggctcgccg agcaggggtg
     1501 gctcctctgc gacggcagcg aggtctccaa gacccactgg cgggagctct acgagctcat
     1561 tcaggacacc tatggaaagg ccggctccgc cgacagcttc gtcctgcccg actaccgggg
     1621 cgccttcctg cgcggcgtca atggcgaccg gagccccgcc ctcgatcctg ccgccggcca
     1681 gcgcaccgcc tcgggcgaag gaggcaccgg cacgggcaac caggtcggct cgttccagac
     1741 ggcccagttc caggagcacg agcaccaata caccgccggc acgaccatga gcgcctttca
     1801 ggccggcggc gtgaacgccg tcatctcgac gatcgagtcg accaccaccg ccgtggtctg
     1861 cgggcagggg agcggggaca gcacctcctg ctacggcgcc gagacgcggc cggtcaatat
     1921 ctatgtcaac tatctcatca agggggtgga tcaccagctc cgcctcacgg ttcccatgga
     1981 gatcccccgg ctcgatcccc gtctcggatt gatccgctga gcgccgctgc cggcacttca
     2041 caccgcccct ccctgcctcg tctcctcttt cgaaaaccca tcaaacaccc gggcggcgag
     2101 ccgcccggga cttttcgagg agggaagaca aatgtcgaga cgagttgtct atccgggctt
     2161 ggccatccac gccgttctgt tcttgttggc acttgccgcc ggtgcccagc caccggtcaa
     2221 gaatcagaac aatatccggg tcacctttgt gaatatcggc gccggcctgt gcacggtgat
     2281 cgagtgcccg ggccttcctg cccagaccgc gccgatcctc tacgactgcg gatcgagcgg
     2341 ccgcggcgac acgggcctcg accgcgccgg caccgtgcag ttcgtccgca cgatcctcag
     2401 ccgctacggc gtcgcgccca gagtcgtcgt cagccatccg gacggcgatc actacaacta
     2461 catcacctcc atcatgggaa atgcgaacgc gagctccatc tggttggggg cgcagacggc
     2521 cgactacaac atcaccttcc agaactggct gatggctcag cagcaggcag gggtggcgat
     2581 caacgtgttc gctcaagggg cctccaacaa cggccagcct gtcggtggtc tcgcctgcgg
     2641 cgccggtctg ctcccgaact cgggaacctg gctgctgacg gtcaacgccg gagacgatga
     2701 caatgccaag agtatggtcc tgcgcctcgc ctacgggcag ttccaggcca ccctgacggg
     2761 cgatgccacc gccgagagcc tgacgagcct catgtacaac taccgggcca acgtcgccaa
     2821 cggttcgatc gccacgacgc tgctcggagc gccccatcac ggctcgatca ccgaggggag
     2881 caacagtttc gtctggcctc aggtcttcca gccacgcatc gtggtctaca gcgccgggga
     2941 gcgctattac cacccgcgct gcgaggcgct cgacgtctac gacgacgccg gcacgctgcg
     3001 gaacgcccag aaccacccgc tgcagtgcgg cgacgagggc gcctactggc tgctcgatca
     3061 ggccaacgcc caatacctga ccgagagcaa cggcagtgtc tgggtcgacg cgaacggcca
     3121 gggtggagtg caggtcacct gcgacgccgc gaacgtcaac tgcaacctca actagccgtg
     3181 gacttcacaa tcgaacggcg gcagcgtcta cgggttgcac ctccaatgac tgacggaagg
     3241 ctcacaacgc cgctctcacg aaaggaacgc acgatgagta ctctgagaac cgccacattc
     3301 gccgccgccc tgaccgtttc cgtcgtcctc gggggctgcg agtacaatcc caccgctccg
     3361 gcggaggtca aggacccctc tccgagcgcc aaccccagct cctgcgaggc cggtccgtac
     3421 cgtatccacc tgaacggcca gtgccgggtc gccaccggca gacgcatcgt ttgcgaagac
     3481 gagagcacca ccacgcccgc ggataaaatc ctcggcatca ccttccagct ccgggacgcc
     3541 gccaccggcg ccccggtgga aacccgatcc gtcagctctg gcgtgatccc ccgggagatc
     3601 tccttctccg gccgggcacc tggctcctat gaggtccgtc ataccgtgca ggcccgcgat
     3661 tgcaccttca tggccaccac ctactccggc ctggaggtgt tctgaacgcg ggatgtccct
     3721 ttcctcgcgc cgaatccgca tctccctgta gccccccttc ttcgggagct ctcgtggaca
     3781 aatccgtccc ggggatcgga aagcgagcca agccatgaag agaaacgtga agcgcctgac
     3841 cctgaaccgt gagaccctcc gctacctcaa cccgcagcag atgaccgacg ctgccggggg
     3901 agacgtttcc gggcgtatct gccccgtgac cgatccctgc ttcgtgacct acttcaactg
     3961 cccgcctccc acgacggtct cccagcagac ctgtccgacc aactgcggtc agaactactg
     4021 ctgagcggcg gacgtcccgc cagcacctac gacgtctcca cgggagcccg gagcgccaca
     4081 cggcgctccg ggcgcctttc tgcttctccc tcgccatccc gcggcgctcc gccaggggca
     4141 ccgcagtcga ctcgcgtact tcaattgtca aagagctgag caagagcgcc tgaaacaagc
     4201 ttgactctcc cgtgctgacg gtgagtgtag catggagggc gttgtgtgag acttagttga
     4261 cgctctgggg acatgctttg tagtctctgc ccctgcgcag ccgcgagaga gagccgcggc
     4321 gtagcgcttc gccgccgttc ctcaacgttg aggggcagtc cctcaccact gaggagccgt
     4381 ccctcaatgc tccgcggtga cttcggactg ctgaggggcc gcggcggaag ggtgagggac
     4441 ggcggcgaag cactgaggag acggcccgga acgcttcggc gtcgcccctc agtggtgagg
     4501 agtcgcggcg aagcattgag gagtcgcggc gaagcaccga gggacggctc ctcgggactg
     4561 aggagccgct cctgaaagct ccggagccaa gcctcaccac tgaggggcga gccctcaacg
     4621 cggagggagc ggcccggaac acttcgggtt gatccctcag tccgagggat cgccccgaag
     4681 tgttgaggga ttgcggccaa gcgttgagga acgccgcctc aacgctgagg agccggcccg
     4741 gatgagaagg aagagccgcc gttccggacc ctcactggag ccgaatcggc ggctccggtt
     4801 cattgacaac tgaatcgtga tggggtgccg ccggcttccg tcgccgatgt tcctggatcg
     4861 atcgcgtact acgacgcgct cgcctttcga aaggatcaac ccgccttcgt ggaatcgatc
     4921 ctcgggcttc cgtgtgccgc cggccaggcc tgaggggatc gcccatctct cttcgtttgc
     4981 agaatgtgac cgaatggtcc gggtgacccc tgcccggacg gtcgggagag gaggcagatg
     5041 gcggtaagga agtctcgtca tcacagtccc cattcaggag agtatctgca ctcttcctgt
     5101 gcacgggagg gtgaggcgct cttgctttag agcttgaagt agcgagtggt aagggcttcc
     5161 tcttggccgg atgaatcgag ctggtccggg aaggaatggc ccgcatgcgg cgttgtactc
     5221 tcctgactcg aagcccgcgc ccggcccagc cacggcttct tcgactccac tgatcggccg
     5281 ggcctggaag acttactgga acgtccgaga tttatcagct ttcctagcct ccgccgccgt
     5341 ctccccaggt tatccggact ttgaagagcg cattttttgt gccgtggact tgatatgctg
     5401 aagaatgcaa cgattgccga aattctagag agccgggcga cggaacaggg cgagcgctcg
     5461 gcgtacgtct tcttgtcgta tggcgagacc ggtgtggtcg aggagcgact cacctacgcg
     5521 gatctcgaca accgagcccg ggcgatcggc gcgtcgttgc aggcggcggg tgccggcggc
     5581 gagcgcgtcg cactgctact gcctcccggt ccggacttcg ttgcctcctt cttcggctgc
     5641 ctctatgccg gcgccgtcgc ggtcccctcg ctgccgccgc gccgtcgcgg tgccgacccg
     5701 cgcctcgccg cgatctgccg cgacgcgcgc ccgcgggtgg cgctgaccac cgacggccag
     5761 ttgcccgccc tggagagcgc cgccgccgag atccccgagc tcgcggccgc cctgcggatg
     5821 gctcccgccc ccctggggac ggcgggggcc gcggactggc ggcggccgga cctcggttcg
     5881 gaggcgctcg ccttccttca gtacacctcg ggctcgacct cgactcccaa gggggtcatg
     5941 gtcagccacg gcaacctctt gcacaacgag gagctgatcc gccggacctt cgagcagtcg
     6001 tccgactcgg tggtgctggg ctggctgccg ctgtaccacg acatgggtct catcggtacg
     6061 gtgctgcagc cgctctacac cggcgcggtc tgctacctga tgacccccgg gtcgttcctg
     6121 cagcggccgg cgcgctggct ggaagccgtc tcgcgctacc gggcgacgac cagcggcggc
     6181 cccaacttcg cctacgagct gtgcgtgcgc aaggtggggg aggcggagcg ggaggggctc
     6241 gacctctctt cctggcgggt ggccttcaac ggcgccgagc cggtgcgggc cggcacgctg
     6301 cggcggtttg ctgacgcctt cgccccctgc ggcttccggg ccgaggcgtt ccgtccctgc
     6361 tacggcctcg ccgaggcgac gctgctcgtc tcgggctggc gccaggaggg ggagacccag
     6421 gtgcgcgcgc tcgacgcgga ggccctggag cggcacgagg tcctgggctc cggagacgcc
     6481 acgcggagcc gcgagctggt gagctgcggc gcggcgatgc agaccatgtt ggccgtcgat
     6541 ccggagtcgg gcgagccttg cccgcccggc cgtgtcggcg agatctgggt ggccggcccg
     6601 agcgtcgccc aggggtactg gcagcggccg gaggagacgg cggcgacctt cgctgcccgt
     6661 ctcgccgacg gcaccggccc tttcctgcgc accggggacc tgggcttcgt ctccgaaggc
     6721 gaggtcttcc tcaccggccg tctcaaggac ctgatcatcc tgcgcggcag gaaccactat
     6781 ccgcaggacc tggagctgac cgccgaacgc agccacgccg ccctgcgcgc cggtggcggc
     6841 gccgccttcg cggccgatcg ctccggggag gagcggctgg tcatcgtcca cgaggtggag
     6901 cggcacgccc gcttcgacgc ggagggtgaa gggaccgagg agatcgccgc cgcgatacgt
     6961 cagagggtgg ccgaagagca tgaggtaagc gtcgccgagg tggtgctgat ccggccggag
     7021 acgctccccc ggacgtccag cggcaaggtg cggcggagca cctgccgcga cctctatctc
     7081 cagggcgggc tgcgcgtgat cggcgcgagc cacctctcgc cggcggctgt ggacctcgac
     7141 cctgcatccg aggtccaggt gggatcgccg gactggctgc gccgcgcctt cgccgccgcg
     7201 gcgcggatcg acccctcccg ggtcgacgcg gacctgccgc tcgccgcctc gggcctggac
     7261 tcgctggcgg ccgtggagct caagcagacg gtggaggagg cgaccggcgt ctccctcccc
     7321 ctggcggacc tgctggaggg gatgaccctg cgcgagctcg atcggcggct ggccgggagc
     7381 accgagaggc tccctgcagg ggaggggctt gcccccttcc gggagggggc aagcccctcc
     7441 cctgcatcga atcaggtggc gtcgggcgag catcccctct cctggaacca gcgctcgctc
     7501 tggttcctcc accgcctggc gccggagagc tccgcctaca acatcgccgg cgcggcgcgg
     7561 ctcgaggccg cgtccttcga agccctgggc cgcgcgatcc aggcgctggt gaaccgccac
     7621 cccatgctgc gcgcgacgtt cgccgatctg cccggcggtc cggtgcagcg actgccggag
     7681 acgacggagg gatccgaagc gcccctcgag gtcgtggacg cgaccggctg gagcgacgcg
     7741 gaggtggacg cccgcctgcg cgccgaggcc tatcgtccgt tcgatctcgc ggccggcccg
     7801 ctcctgcgga cggtcctgct gcggcgtggg gacgaggcct tctttgcctt cgccgtgcac
     7861 cacgtcgccg ccgacttctg gtcgatggcc gtgctggccc gcgagctcgg cgcgctggtg
     7921 gccggggaga ccctgccccc tccggcggtc ctgtacacgg acttcgcgca ctggcaggag
     7981 cggatgctgg agagctccgc gggcgagtgg ctctgggagc actggcggga gcgcctggcc
     8041 ggcacgccgc agctcgacct gcccaccgac cggccgcggc gtccggtgca ggccctgcgc
     8101 ggtggcgcgc ggacgctccc cccctcgctg gagcgggcgg aggcggtcca gcggctcgcc
     8161 gccacccatg gctgcacgcc tttcgtcgcc ctgctcgcca tctggcaggc cgtgctctcg
     8221 cgctggagcg gccaggagga gttcctcacc ggcgccccca tggccggccg ctccgcccgc
     8281 gaatggggag aggtggtggg ctacttcgtc aacctggtcc ccttgcgcgc cgacctctcg
     8341 ggtgatctgg gagcccacga gctgatggcg cggacgcgcc gcaccgtgct cgacgcgctg
     8401 gcgcaccagg atttcccctt cgccctgctc accgagcgcc tgcagcccga gcgcgacccg
     8461 agccggccgc cgctggtcgc ggccatgctg accttggaga aggccccggc gccggagctt
     8521 gccgccctcg cggccttcgc cgtcggcatg ccgggcgtcc ggctcgacct gggaggggtg
     8581 ggtgtcctgg aaacgctcgc cctggcgccg ccggcggccc agctcgatct ctccctgacc
     8641 gcggccgagc tccccggtgg gctcgccctc tccttgcagt gggacgccga cctgttcgac
     8701 gccgcgacca tcgaccgcct gaccgggcac ttcgagcggc tgctcgtcga aatggccgtc
     8761 gcggaccctc gcccggtcgc ggagctgccg ctgctctccc cggccgagcg ccaccagatg
     8821 ctcgcggagt ggaacgacac ggcgctgccg cggtcccggg gcgccctcct gcaggacctc
     8881 ttcgaggcgc aggcggcccg cacgcccgag gcgccggccg tggctttcca gggggagacc
     8941 ctcacctacg ccgagctcgg cgcccgcgcc ggccggctgg cgcggcacct gcggcggctg
     9001 ggatgcggcg cggagagccg cgtcggtgtt gccctggagc gctccctcga cctggtcgtc
     9061 gcccttctcg gcgtgctcga ggccggcgcc gcctacgtgc cgctcgatcc cggctacccg
     9121 cgcgagcggc tggtcctggt gctggaggac gcggcgccgc aggtgctgat taccgagacc
     9181 ggtctgcgcg ccgcgctccc cgcggctgcc gggacggcgg tcctctgtct cgacgccgag
     9241 gcgctcaccg agggtccgct cgaccgggtg gccggggacg accgccagct cgcctacgtc
     9301 ctctacacct cgggctcgac gggccggccc aagggggtgg gcgtgccgca cgaggcgctg
     9361 gtgaatttcc tcggatcgat gcgccgggcc ccgggcttct ccccggggga gcggctgctc
     9421 gccgtcacct cgctgagctt cgacatcgcg gcgctggaga tcttccttcc cctgatcacg
     9481 ggcggctgcg tcgagctggc cagccgggtg gaggccgcgg acgccgcgct gttggcggac
     9541 cggctccgcg cttccggagc gggcgtcctg caggcgaccc cggcgacctg gcggatgctc
     9601 ctcgactcgg gttggcccgg cgatccccat ctgcgggcac tgtgcggcgg cgaggggctg
     9661 ccccgcgatc tggccgagtc tctcgccggg cgcaccggcg agctgtggaa cctctacggc
     9721 ccca
//
LOCUS       DASPJI010000558         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_383444, whole genome shotgun sequence.
ACCESSION   DASPJI010000558 DASPJI010000000
VERSION     DASPJI010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383444"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VEL74_15455"
     CDS             complement(<1..594)
                     /locus_tag="VEL74_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HYG63971.1"
                     /translation="MSSGGHGGRRRGKKHEEEEHENHERWLVSYADMMTLLLVLFVVL
                     YAMSQVDQQKFAALAKGLAASFGGPVTVQPGPTPEGSVLDGLPGAVDIASAIVPDQTV
                     EQAEVDAAAAQAALAEARQVAAEARAEYEDLSEVREQIEAALAAAGHAGAARFEIDER
                     GLVVHIVADQVLFDAEQAVLRPEGRQILDAIAPTLRGL"
     gene            complement(626..1396)
                     /locus_tag="VEL74_15460"
     CDS             complement(626..1396)
                     /locus_tag="VEL74_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HYG63972.1"
                     /translation="MDPATLIGFVVSLVALLIFMVMEGADPTSLLFLPAIILVIVATF
                     GAAMSSQTMDDLKKLPAWFKMAVMPAKVPPATEQIQTLVTLAERARKEGLLALEAQVR
                     AIEDPFLKRGLQMGIDGTDPEELRAVLESEISAKKAEDKVAAKFMNAMGGYAPTIGIV
                     GCIVGLMNVMGNLSNPEALGPMIAAAFVATLWGVMAANFWFLPMGAKILRVSELQAAQ
                     MELLVEGITEIQAGTSPRAVRLKLTSLIPPSEVAKEAA"
     gene            complement(1400..1684)
                     /locus_tag="VEL74_15465"
     CDS             complement(1400..1684)
                     /locus_tag="VEL74_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HYG63973.1"
                     /translation="MIRVTRLNGERFALNPDLVERVEGHPDTVVFLVDGTKYIVSESV
                     DEVLVEIREYRASILATAYEMDRGTYRSPFRTTDEDAHAAVVPFPAREER"
     gene            complement(1959..>2063)
                     /locus_tag="VEL74_15470"
     CDS             complement(1959..>2063)
                     /locus_tag="VEL74_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HYG63974.1"
                     /translation="EFTGLIVAQRGFQANSRVISSSDEVLQDLVNLKR"
BASE COUNT          283 a          720 c          744 g          316 t
ORIGIN      
        1 cagcccgcgc agcgtggggg cgatcgcgtc gaggatctgc cggccctcgg gccgcagcac
       61 cgcctgctcg gcgtcgaaca ggacctggtc ggccacgatg tgcacgacca gaccgcgctc
      121 gtcgatctcg aagcgggccg caccggcgtg gccggcggcg gccagcgcgg cctcgatctg
      181 ctcccgcacc tcggagaggt cctcgtactc ggcccgggcc tccgcggcga cctgccgcgc
      241 ctcggcgagc gcggcctgcg cggcggcggc gtccacctcc gcctgctcca cggtctggtc
      301 cgggacgatg gccgaggcga tgtcgaccgc accgggcagc ccgtcgagca ccgagccctc
      361 cggcgtcggt ccgggttgca cggtgaccgg cccgccgaag ctcgccgcca ggcccttggc
      421 cagcgccgcg aacttctgct ggtcgacctg gctcattgcg tagaggacca cgaacagcac
      481 gaggagcagc gtcatcatgt ccgcgtagga caccaaccac cgctcgtggt tctcgtgctc
      541 ctcctcctcg tgcttcttgc cgcggcggcg gccgccgtgg ccgccggaac tcaccgcgcc
      601 ccctctggtg agctcgcgag cccgctcatg ctgcctcctt ggcgacctca ctgggcggga
      661 tgagggaggt gagcttgagc cggaccgcgc gcgggctggt gccggcctgg atctcggtga
      721 tgccctcgac cagcagctcc atctgcgcgg cctgcagctc gctgacccgc aggatcttgg
      781 cgcccatcgg caggaaccag aagttggcgg ccatgacgcc ccacagcgtc gcgacgaacg
      841 ccgcggcgat catcgggccc agtgcctcgg ggttgctgag gttccccatc acgttcatga
      901 ggccgacgat gcagccgacg atgccgatgg tcggggcgta gccgcccatc gcgttcatga
      961 acttggcggc caccttgtcc tccgccttct tggcggagat ctcgctctcc aggacggcgc
     1021 gcagctcctc ggggtcggtg ccgtcgatgc ccatctgcag cccgcgcttg aggaacgggt
     1081 cctcgatcgc cctgacctgc gcctccagcg cgagcaggcc ctccttgcgg gccctctcgg
     1141 cgagggtcac cagggtctgg atctgctcgg tcgccggcgg gaccttggcc ggcatcacgg
     1201 ccatcttgaa ccaggcgggc aacttcttga ggtcgtccat cgtctggctg gacatcgccg
     1261 cgccgaaggt ggcgacgatg accaggatga tcgcggggag gaagagcagc gacgtcgggt
     1321 cggcaccctc catgaccatg aagatcagga gcgcgacgag ggagacgacg aacccgatca
     1381 gggtggcggg gtccacgggt cagcgctcct cgcgggccgg gaaggggacg accgcggcgt
     1441 gcgcgtcctc gtcggtggtg cggaacggcg accggtacgt gccgcggtcc atctcgtagg
     1501 cggtggcgag gatgctcgcc cggtactcgc ggatctccac gagcacctcg tcgacgctct
     1561 ccgagacgat gtacttggtc ccgtcgacca ggaacacgac ggtgtcgggg tggccctcga
     1621 ccctctcgac gaggtcgggg ttgagcgcga accgctctcc gttcaggcgc gtcacacgga
     1681 tcacgggggt cctctagctc gctcggggag ggggtggcgg cgaccgctcg aggggctgcc
     1741 agtctgcagt tgtcttcggc gtggcgcggg cccgccttga gccgaacagg ccgcctgacc
     1801 cgtgcgggtg aggaggtgac ctgcgtggac gccggggcgc gaggacgacg gcggcccggc
     1861 tcctcgcgag gagggaccgg gccaccggga cgtgctggaa cggcctcctc ccaggggccc
     1921 gccacgagcc cgcgagtggt ggggaggagg gaggtccttt accgcttcag gttcaccagg
     1981 tcctgcagca cctcgtcgga gctggagatg acgcggctgt tggcctggaa gccgcgctgg
     2041 gcgacgatca gcccggtgaa ctc
//
LOCUS       DASPJI010000559        10441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_262711, whole genome shotgun sequence.
ACCESSION   DASPJI010000559 DASPJI010000000
VERSION     DASPJI010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10441)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10441
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262711"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VEL74_15475"
     CDS             complement(<1..915)
                     /locus_tag="VEL74_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HYG63975.1"
                     /translation="MQNDGPYVSSFGLDKQGITGVRRVYWNLPPARLYEMAIQRGEAM
                     TASEGPLVCDTGQHTGRSPNDKFLVRDPSVDADIWWGDVNKPFEKEKFDALLARAQEY
                     LQDRDVWVFDGYAGADPRFRLNVRVITETAWHNLFARNMFIRPGAAALADFEPAFTIL
                     HAPGLTARGKDDGVNSGTFILVDFDRRVILIGGSWYAGEIKKSIFGVLNYLLPEQGVM
                     PMHCSANVGPKGDVAIFFGLSGTGKTTLSADGTRTLIGDDEHGWSENGIFNFEGGCYA
                     KTIRLSAEAEPEIYATVRRFGTVLENVPL"
     gene            complement(966..2321)
                     /locus_tag="VEL74_15480"
     CDS             complement(966..2321)
                     /locus_tag="VEL74_15480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HYG63976.1"
                     /translation="MTAPRKALRHTALLPALLLGMLSATAAGVSHAQATQAPAAAPLQ
                     EIQQEIRRRTDEVAPRIVEVRHRLHQNPELSNREVETAKLVAEHLRGLGLEVRTGIAK
                     TGVTARLVGGRPGPLIAVRADIDALPVTEETDLPFRSTKRDTFMGQEVGVAHACGHDI
                     HTSVQLGVASVLTAMRDRIPGTILFIFQPAEEGPPAGERGGADLMLEEGVFKDGKPAA
                     IFALHAFPDLPLGQVGYNPGPTMAAVDQFVIKIKGRQAHGAYPHLSVDPVVTAAQAVL
                     ALQTIRSRNLSAFEPSVVTVGIVRGGERFNIIPGEVHLEGTVRTYNSQAREDVERRMR
                     EILDGVTRAAGATFEMEYKKNAPATVNDPGLTRTAGALLQSALGADNVKVVEPSMAGE
                     DFAYFANEVPGFYYRLGVVRPGTTSGGLHTPTFRADDGAVAVGIHAMARLLVDFLHER
                     R"
     gene            2554..4182
                     /locus_tag="VEL74_15485"
     CDS             2554..4182
                     /locus_tag="VEL74_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HYG63977.1"
                     /translation="MNIENRKILVLGGYGLVGMAVCRELLVRKPREIQIHSLRMEESE
                     RACEELAREANGTKLTPSAGNIFGLVQGGTRRELIRAELQRLADEDLTSFVLYNVLAE
                     ARPDAVIDCVNTATGIAYGDIFKSAERVLSQLEGDALDAETTERLLDALYMPRLIRHI
                     QVMYRGMIDAGTRVYVKVGTSGTGGMGLNIPYTHSEERPSRVLLSKSAMAGAQSMLLF
                     LMARTPGAPITKEIKPAAAIAWKKIGYGPIRRGGQPLHRVEARPAPLGATFSTEDGAA
                     ARMLDAPYENVFIDTGENGVFSLEEFVTVTSEEQMEFVTPEEIARYLVFEIEGGNTGY
                     DIVSALDGAVLGPTYRGGVLRHWAIEHMTELERQHGVRSVAFEMLGPPRNSKLLLEAH
                     FLRCAFPSMDAVRESTPEAVRDTLNRLARERADLVQDALAVGIPILLDSGEVLRGPKV
                     LVPADAVDEPVTPAKLEEWVRDGWVDLRLANCTRWIERFQKIHDQTAALPEADTSSRY
                     LRNREFWYREPEIQPGKVVGWILSSEEGGWRMKR"
     gene            complement(4184..5293)
                     /locus_tag="VEL74_15490"
     CDS             complement(4184..5293)
                     /locus_tag="VEL74_15490"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HYG63978.1"
                     /translation="MRRYEWTQWLDVPLPGGRVGHWLLAAAVFLLFFAVFALLRRLVV
                     RRMAAVASRTETDVDDFVVDVLRRTRRWLLLLPAAYLASLVLTLPAQIREGLRGTAIL
                     SLLIQVALWALVGIDFGVERTRKKRLAVDSTSVALLGVLRFVGKIVLWTIVLLVALDN
                     LGVDITALVTGLGVGGIAVALAVQNILGDLLASLSIVLDKPFVVGDAITVGEFTGTVE
                     SVGLKTTRLRSVNGEQIIFPNGDLLQSRIRNWGRLTERRVALAFGVVHGTPPELVEAV
                     PGMIREAVESRDLLRFDRAHFKGFTPTALDFEAIYWVTTPDYAAFMDRQQAINLELLR
                     RFEAAGIHLAAPTPAVIVEKVERVERVGAEPRPSR"
     gene            complement(5343..6488)
                     /gene="amrS"
                     /locus_tag="VEL74_15495"
     CDS             complement(5343..6488)
                     /gene="amrS"
                     /locus_tag="VEL74_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HYG63979.1"
                     /translation="MQYQEVNDTVPTKYWHLLENGRIQCDLCPRFCKLLEGQRGLCFV
                     RARENDQIVLKTWGRSSGFCVDPIEKKPLNHFLPGTPVLSFGTAGCNLACKFCQNWDI
                     SKSREVDTLADQATPEVIARAAERLGCASVAFTYNDPVIFMEYAVDVAEACHAAGIQT
                     VAVTAGEICPEPRAEFYRHMDAANVDLKAFTERFYRKICGGELGAVLDTLRYLKHETD
                     VWFEITNLLIPGENDSEQEIDQMTRWIVDELGPDVPIHFSAFHPDWKMLDYPSTPAST
                     LTRSRRIALANGIRYAYTGNVHDEDGGSTYCHGCGETLIGRDWYVLSEWNLTPEGACA
                     SCGTPCAGRFQPRPGAWGARRVPVRLRDFAPAHSADSALASPLRILA"
     gene            complement(6626..9211)
                     /locus_tag="VEL74_15500"
     CDS             complement(6626..9211)
                     /locus_tag="VEL74_15500"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HYG63980.1"
                     /translation="MNTPPRSRCARVRRPRALFAALALLSVTLAAPLAAQAPADLFIS
                     EYIEGSSNNKALEIFNGTAAAIDLGAGGYSVQMFFNGSASPGLTINLTGTVAAGDVFV
                     LAHGSAVAEVLAAADQTNSAGWFNGDDAVVLRKGGSIIDSFGQVGSDPGTEWGSGLAS
                     TADNTLRRKVSACAGDPSASDLFDPATEWDGFATNAFDGLGAHACVVVPPPPPVELEV
                     FEIQGNGLASPYAGKTVLALDNVVTAVGPQGFFIQAPDDRADADAATSNGIYVFTNSA
                     PSVSVGDQVDVQGTVTEFFDLTEITNPTVTVDSTGNPLPAVVLFSASLPSPVPQPVAE
                     LERYEGMIVRVENGVATAASDRFGDFALVAGSDRPFREPGILFPGLAGLPVWDGNPEI
                     FEIDPDKLGLPNVVVPGGATVDVAEGPLAFAFGDYQIWPTELTVNGEPHLEPVRDRQP
                     GEFTVASQNLLRLYDTVNDPATSDEVASAQTYANRLNKASLLIREGLGSPDVLALQEI
                     ENLTVLQDLAAKLQADAPSLVYTPYLLEGNDIGGIDVGFLVRDTVRVDSVVQFGADTP
                     FTFNGSTALLNDRPPLVLRGAYLANGAPFPITVIAVHQRSLSGIEGSDGDRIREKRHQ
                     QALTLSQYIQSLQAAEPGLRLVVTGDFNAFEFTDGFVDAMGQLTGSPDPAGALIPATD
                     EIDPDLTNQLLSEPPAERYSFLFDGSAQALDHALTSQALNPFVRGLDHVRGNADTPDF
                     FASIPTTALRTADHDGLVLFLMSDSDADGIAEDADRCPGTVIPEGIAKLNPNHWALVD
                     ADLVFDTRGNAAPARTFTVADTAGCSCTQIVAALGLGQGQLKNGCSNGTMADWVALVS
                     PLVSQ"
     gene            complement(9276..10424)
                     /locus_tag="VEL74_15505"
     CDS             complement(9276..10424)
                     /locus_tag="VEL74_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HYG63981.1"
                     /translation="MPRYPGYSPSVDHIAGAVYSSLAHRLESYKGEVYPLHVGDTWME
                     PPAGCRMEDLRVSDYPGMHRYAPPQGLPALLDAIAERVQARSGAPTARENVLVATGAT
                     GALGAVAGAILAPGDEVLQLAPHWPLITGIIQSFHGVPVDVPFFGRADSPETAVEAVR
                     ELRTDRTVALYLNTPSNPTGRIIPRAWIEALVEWARSEDLWVISDEVYEDYLFEGEHA
                     YTRPLAPERTFAAHSFSKAYGMAGNRCGYVVGPAAAMKELRKVSLHSFYSTPTAAQIA
                     ALRVLSGPGEPWIAEARQSYRQAGLKAAARLGIEPPQGSTFLFLDVADRLDHRGLQGL
                     LEDCVDRGLFVAPGPSFGPYPTHIRLCFTAAPPDVVERGVEALASVLK"
BASE COUNT         1589 a         3464 c         3846 g         1542 t
ORIGIN      
        1 gagcggcacg ttttccagca ccgtgccgaa gcgccgcacc gtcgcgtaga tctcgggctc
       61 cgcctcggcc gagaggcgga tcgtcttggc gtagcagccg ccctcgaagt tgaagatgcc
      121 gttctccgac cagccgtgct cgtcgtcgcc gatcagggta cgggtgccgt cggccgacag
      181 ggtggtcttg ccggtgccgg agaggccgaa gaagatcgcg acgtcgccct tcgggccgac
      241 gttggcggag cagtgcatcg gcatcacgcc ctgctccggg agcaggtagt tcagcacgcc
      301 gaagatcgac ttcttgatct cgccggcata ccagctgccg ccgatcagga tcaccctgcg
      361 gtcgaagtcg accaggatga aggtcccgga gttgacgccg tcgtccttgc cgcgcgccgt
      421 cagcccgggt gcgtgcagga tcgtgaaggc gggctcgaaa tcggcgagcg cggccgcgcc
      481 gggccggatg aacatgttgc gggcgaacag gttgtgccag gcggtctcgg tgatcacccg
      541 gacgttgagc cggaagcgcg ggtcggcgcc ggcatagccg tcgaacaccc agacgtcgcg
      601 gtcctggagg tactcctggg cgcgcgccag cagggcgtcg aacttctcct tctcgaaggg
      661 cttgttgacg tcgccccacc agatatcggc gtcgaccgag gggtcgcgca ccaggaactt
      721 gtcgttgggg gagcggcccg tgtgctgccc ggtgtcgcag accaggggcc cttcgctcgc
      781 cgtcatcgcc tccccgcgct ggatggccat ttcgtacagg cgcgccggcg gcaggttcca
      841 atacaccctg cgcacgccgg tgatcccctg cttgtccaac ccgaagctgc tcacgtacgg
      901 accgtcgttt tgcatgccgt cacctcaaat cagtattcat cgtatcggaa gagagaaacc
      961 cggcctcagc gcctctcgtg caggaagtcg accagcaggc gcgccatggc gtggatgccc
     1021 acggccacgg cgccgtcgtc ggcgcggaaa gtcggggtgt gcaggccgcc ggaggtcgtg
     1081 cccggccgga ccacacccag ccggtagtag aaccccggga cctcgttggc gaaataggcg
     1141 aagtcctccc ccgccatgga gggctcgacc accttcacgt tgtcggcccc gagcgcgctc
     1201 tgcagcaggg cgccggcggt gcgggtgagc ccggggtcgt tgaccgtggc cggcgcgttc
     1261 ttcttgtact ccatctcgaa ggtggcgccg gcggcgcgag tgacgccgtc caggatctcc
     1321 cgcatgcgcc gctcgacgtc ctcgcgcgcc tgggagttgt aggtgcgcac cgtgccttcg
     1381 aggtgcacct ccccgggaat gatgttgaac cgctcgccgc cgcgcacgat gcccaccgtg
     1441 acgacgctgg gctcgaaggc ggagaggttg cgggagcgga tcgtctgcag ggccagcacc
     1501 gcctgcgccg cggtcaccac cgggtccacg gagaggtggg ggtaggcgcc gtgcgcctgc
     1561 cggcccttga tcttgatcac gaactggtcg accgccgcca tggtcgggcc ggggttgtag
     1621 cccacctggc cgagcggcag gtcggggaag gcgtggaggg cgaagatggc cgccggcttg
     1681 ccgtccttga acaccccctc ctccagcatc aggtcggcgc cgccgcgctc gccggccggc
     1741 ggcccctcct cggcgggctg gaagatgaag aggatggtcc ccgggatccg gtcgcgcatc
     1801 gcggtcagca ccgacgccac cccgagctgg accgaggtgt ggatgtcgtg gccgcaggcg
     1861 tgggccaccc cgacctcctg ccccatgaag gtgtcccgct tggtcgagcg gaacggcaga
     1921 tcggtctcct cggtcaccgg cagggcgtcg atgtcggcgc gcacggcgat cagcggcccc
     1981 ggccggccgc ccacgagcct ggcggtgacg ccggtcttgg cgatgccggt ccgcacctcg
     2041 agcccgaggc cacgcaggtg ctccgccacc agcttcgcgg tctccacctc gcggttgctc
     2101 agctcggggt tctggtgcag gcggtggcgg acctcgacga tccgcggcgc cacctcgtcg
     2161 gtccgccgcc ggatctcctg ttggatctcc tggagggggg ccgccgccgg tgcctgggtg
     2221 gcctgggcgt gcgacactcc agccgctgtc gccgacagca tccccagcag cagggcgggg
     2281 agcagcgccg tgtgacggag cgccttccgg ggtgcggtca tcgtatcctc ctcagggcga
     2341 tcgagagccg ggaggggacg taccggcgcc ctctccggga gtggaaaacc cgccgagttt
     2401 acaacgtccg ccgggctttc tcactccgcg ccgctctgcc gcggcgcggc gaagcgttcc
     2461 cgggccgggc cgctgtagaa tccccccgcc aacgggaacg cactccggcg cactgtccct
     2521 ctgaggcact ttccctggat aggagagact gggttgaata tcgaaaatag gaagatcctt
     2581 gtgctgggag gctacggcct cgtcggcatg gcggtgtgcc gggagctgct ggtcaggaag
     2641 ccccgcgaga tccagatcca cagcctgcgc atggaggagt cggagcgggc gtgcgaggag
     2701 ctggcgcggg aggcgaacgg caccaagctc accccctcgg ccggcaacat cttcggcctg
     2761 gtgcaggggg gtacgcgccg cgagctcatc cgcgcggagc tgcagcggct ggccgacgag
     2821 gatctcacct ccttcgtgct ctacaacgtc ctggcggagg cgcggccgga cgcggtcatc
     2881 gactgcgtca acaccgcgac cggcatcgcc tacggcgaca tcttcaagtc ggccgagagg
     2941 gtcctgagcc agctcgaggg ggacgcgctc gacgcggaga ccaccgagcg cctgctcgac
     3001 gcgctctaca tgccgcgcct catccgccac atccaggtga tgtaccgcgg catgatcgac
     3061 gccggcaccc gcgtctacgt caaggtgggc accagcggca cgggcggcat gggcctgaac
     3121 atcccctata cgcactccga ggagcgcccc agccgggtgc tgctgtcgaa gagcgccatg
     3181 gcgggcgcgc agtcgatgct cctcttcctg atggcgcgca cgcccggggc gccgatcacc
     3241 aaggagatca agccggcggc ggcgatcgcc tggaagaaga tcggctacgg cccgatccgg
     3301 cgcggcggcc agccgctgca ccgggtggag gcccggccgg cccccctggg cgccaccttc
     3361 tccaccgagg acggcgcggc ggcccggatg ctcgacgcgc cgtacgagaa cgtcttcatc
     3421 gacaccggcg agaacggcgt cttcagcctg gaggagttcg tcaccgtcac cagcgaggag
     3481 cagatggagt tcgtgacgcc ggaggagatc gcccgctatc tggtcttcga gatcgagggg
     3541 ggcaacaccg gctacgacat cgtcagcgcg ctggacggcg cggtgctggg gcccacttac
     3601 cgcggcggcg tcctgcgcca ctgggcgatc gagcacatga ccgagctcga gcggcagcac
     3661 ggggtccgga gcgtcgcctt cgagatgctg gggccgccgc gcaactccaa gctcctcctc
     3721 gaggcccact tcctgcgctg cgccttcccg agcatggacg cggtgcgcga gtcgacgccg
     3781 gaggcggtgc gcgacaccct gaaccggctg gcccgcgagc gcgccgacct cgtccaggac
     3841 gcgctcgccg tgggcatccc catcctgctg gattcggggg aggtcctgcg cgggccgaag
     3901 gtcctggtcc cggccgacgc cgtggacgag ccggtcacgc ccgcgaagct cgaggagtgg
     3961 gtgcgcgacg gctgggtgga cctgcggctc gccaactgca ccaggtggat cgagcgtttc
     4021 cagaagatcc acgatcagac cgccgccctg cccgaggccg acacctcgag ccgctatctc
     4081 cgcaaccgcg agttctggta ccgcgagccg gagatccagc ccggcaaggt cgtcggctgg
     4141 atcctctcca gcgaagaggg tgggtggagg atgaagcggt aggtcagcgg gagggccggg
     4201 gctcggctcc gaccctctcc acccgctcca ccttctccac gatgaccgcc ggggtgggag
     4261 ccgcgaggtg gatgccggcg gcttcgaagc ggcgcagcag ctccaggttg atcgcctgct
     4321 ggcggtccat gaaggcggcg tagtccgggg tcgtgaccca gtagatcgcc tcgaagtcga
     4381 gcgccgtggg ggtgaagccc ttgaagtggg cgcggtcgaa gcgcagcagg tcgcgggact
     4441 cgaccgcctc gcggatcatc cccgggaccg cctccaccag ctcgggcggc gtgccgtgga
     4501 ccacgccgaa ggcgagcgcc acccgccgct cggtcagccg gccccagttg cggatgcggc
     4561 tctgcagcag gtcgccgttg gggaagatga tctgctcgcc gttgacgctg cggaggcggg
     4621 tggtcttcag gcccacgctc tccaccgtgc cggtgaactc gccgacggtg atcgcgtccc
     4681 ccaccacgaa gggcttgtcc agcacgatcg acagcgacgc cagcaggtcc cccaggatgt
     4741 tctggaccgc cagcgccacc gcgatgcccc cgacgccgag gccggtgacc agggcggtga
     4801 tgtccacccc caggttgtcc agggcgacca gcagcacgat ggtccagagg acgatcttgc
     4861 cgacgaagcg caggacgccg aggagagcca ctgacgtgga gtccacggcg aggcgctttt
     4921 tccgcgtccg ctcgaccccg aagtcgatcc ccaccagggc ccagagcgcg acctggatca
     4981 gcagcgagag gatggccgta ccgcgcagcc cctcccggat ctgcgccggc agggtcagga
     5041 cgaggctcgc caggtaggcg gccggcagca gcagcagcca gcggcgcgtc cgccgcagga
     5101 cgtcgaccac gaagtcgtcg acgtcggtct cggtacggga ggccaccgcc gccatgcggc
     5161 gcaccaccag gcgccggagc agggcgaaga cggcaaagaa gaggaggaag acggcggcgg
     5221 ccagcagcca gtggcccaca cgccccccgg ggagggggac gtcgagccac tgggtccatt
     5281 cgtatcgccg catcgcttcc gcaccgggcg gccaagcggc caccggcgcg gaatgctatc
     5341 actcaggcca ggatgcgcag gggggaggcc agtgccgagt cagcggagtg agcgggcgcg
     5401 aagtcgcgca ggcggaccgg gacgcggcgc gccccccagg cgccgggacg gggctggaag
     5461 cggccggcgc acggggtgcc gcaggaagcg caggcacctt cgggggtgag gttccactcg
     5521 ctcagcacgt accagtcgcg gccgatgagc gtctcgccgc agccgtgaca gtaggtgctg
     5581 ccgccgtcct catcgtggac gttgccggtg tacgcgtagc ggatgccgtt ggccagggcg
     5641 atccgccgcg accgggtgag cgtcgaggcc ggcgtggacg ggtagtccag catcttccag
     5701 tccggatgga acgcgctgaa gtggatgggc acgtccgggc cgagctcgtc cacgatccac
     5761 cgggtcatct gatcgatctc ctgctcggag tcgttctctc cggggatcag taggttggtg
     5821 atctcgaacc agacgtcggt ctcgtgcttg agatagcgca gggtatcgag cacggccccc
     5881 agctcgccgc cgcagatctt gcggtagaag cgctcggtga aggccttgag gtcgacgttg
     5941 gccgcgtcca tgtggcgata gaactcggcc cgcggctccg ggcagatctc gccggcggtc
     6001 accgcgacgg tctggatgcc cgcggcatgg caggcctcgg ccacgtccac cgcgtactcc
     6061 atgaagatga ccggatcgtt gtaggtgaag gcgacgctgg cgcagcccag ccgctcggcc
     6121 gcgcgggcga tcacctcggg cgtggcctgg tcggcgaggg tgtcgacctc gcggctcttg
     6181 ctgatgtccc agttctggca gaacttgcag gccaggttgc agcccgccgt gccgaacgag
     6241 agcaccggcg tccccggcag gaagtggttc aggggcttct tctcgatggg atcgacgcag
     6301 aagccgctcg accgtcccca ggtcttgagg acgatctgat cgttctcccg ggcgcggacg
     6361 aagcacagcc cccgctgccc ctcgagcagc ttgcagaagc gcgggcagag gtcgcactgg
     6421 atgcggccgt tttccagcag atgccagtat ttggtgggga cggtgtcgtt cacttcctgg
     6481 tactgcaagg ggtactgcct ccgggtcagc ctttacaatc cgaaaacatc ccgggcagga
     6541 agcgggattc ccctctagcc tgaaaagaaa agggccggag ggatctctcc ctccagcccg
     6601 atcactttac aggcggacgc ggcggttact ggctgaccag cgggctgacc agcgccaccc
     6661 agtccgccat ggtgccgttc gagcagccgt tcttgagctg accctggccg aggccgagag
     6721 ccgcgacgat ctgggtgcag gagcagcccg cggtgtcggc gacggtgaac gtccgggccg
     6781 gagcggcgtt gccacgggtg tcgaacacca ggtcagcgtc gaccagggcc cagtggttgg
     6841 ggttgagttt ggcgatcccc tccgggatca ccgtgccggg gcagcggtcc gcgtcctcgg
     6901 cgatgccgtc ggcgtcggaa tcgctcatca ggaagaggac caggccgtcg tggtccgccg
     6961 tgcggagcgc ggtggtgggg atgctcgcga agaagtccgg agtgtcggcg ttgccgcgga
     7021 cgtggtccag gccgcgcacg aacgggttca gggcctgcga ggtcagcgcg tggtccaggg
     7081 cctgggcgct gccgtcgaag aggaaggagt agcgctccgc cggcggctcg gataggagct
     7141 ggttggtgag gtcgggatcg atctcgtccg tcgccgggat cagcgcgccg gccggatcgg
     7201 ggctgccggt gagctgcccc atggcgtcga cgaagccgtc ggtgaactcg aaggcgttga
     7261 agtcgccggt gaccaccagc cggaggcccg gctcggcggc ctgcaggctc tggatgtatt
     7321 gcgagagggt cagcgcctgc tggtggcgct tctcgcggat gcggtcgccg tcggagccct
     7381 cgatgccgga gagcgaccgc tggtgcacgg cgatgacggt gatggggaac ggcgcgccgt
     7441 tggcgaggta ggcgccgcgc agcaccagcg gcggccggtc gttcagcagg gccgtgctgc
     7501 cgttgaaggt gaacggggtg tcggcgccga actgaacgac ggaatcgacc cgcacggtgt
     7561 cgcgcaccag gaagccgacg tcgatgccgc cgatgtcgtt gccctccagc aggtaggggg
     7621 tgtagaccag cgatggggca tccgcctgga gcttggcggc caggtcctgg aggacggtca
     7681 ggttctcgat ctcctggagc gccaggacgt cgggcgagcc cagcccctcg cggatcagga
     7741 gcgaggcctt gttcagccgg ttcgcgtagg tttgcgccga ggccacctcg tcggaggtcg
     7801 ccggatcgtt gaccgtgtcg tagaggcgga gcaggttctg gctcgccacg gtgaattcgc
     7861 cgggctggcg gtcacgcacc ggctcgaggt gcggctcgcc gttgacggtc agctcggtgg
     7921 gccagatctg gtaatcgccg aaggcgaagg cgagcggccc ctcggccacg tcgacggtgg
     7981 cgccgcccgg aaccacgacg ttcgggaggc ccagcttgtc ggggtcgatc tcgaaaatct
     8041 cggggttgcc gtcccacacc ggcaggccgg cgaggcccgg gaacaggatg ccgggctcac
     8101 ggaacggccg gtcggagcct gccaccaggg cgaagtcgcc aaaacggtcg gaggcggcgg
     8161 tcgcgacgcc gttctcgacg cgcacgatca tcccctcgta gcgctccagc tccgcgaccg
     8221 gctgcgggac gggcgagggg agggacgcac tgaagaggac caccgcgggc agcgggttgc
     8281 cggtggagtc caccgtcacc gtcgggttgg tgatctcggt gaggtcgaag aactccgtta
     8341 ccgtgccctg gacgtccacc tggtcgccca cggagacgct cggcgcgctg ttggtgaaga
     8401 catagatgcc gttggaggtc gcggcgtcgg cgtccgcccg gtcgtccggg gcctggatga
     8461 agaagccctg ggggccgacg gcggtgacca cgttgtccag ggccaggacg gtcttgccgg
     8521 cgtaggggga ggcgaggccg ttcccctgga tctcgaacac ctcgagctcc acgggcggcg
     8581 gcggggggac cactacgcag gcgtgggcgc ccaggccgtc gaaggcgttg gtggcgaagc
     8641 cgtcccactc ggtggccggg tcgaagaggt cggaggcgct gggatcgccg gcgcaggccg
     8701 agaccttgcg gcgtagcgtg ttgtcggcgg tggaggcgag gccgctcccc cactcggtgc
     8761 cgggatcgga gcccacttgg ccgaaggagt cgatgatcga gccacccttg cgcaggacca
     8821 ccgcgtcgtc accgttgaac cagccggcgg agttggtctg gtcggcggcg gcgaggacct
     8881 ccgccacggc cgagccgtgg gcgagcacga agacgtcacc cgcggcgacg gtgccggtga
     8941 ggttgatggt caggcccggc gaggcgctgc cgttgaagaa catctgcacc gagtagccgc
     9001 cggcgccgag gtcgatggct gcggcggtgc cgttgaagat ctccagggcc ttgttgttcg
     9061 agctgccctc gatgtactcg gagatgaaga ggtcggcggg ggcctgcgcc gcgagaggcg
     9121 cggcgagcgt gaccgacaac agggcgaggg cggcaaacag cgcgcggggg cgacggaccc
     9181 gcgcgcacct cgagcgaggt ggcgtgttca ttcgtggaga ctcctcgtgg atcagaggtt
     9241 gtgagacaga gcccggcagg gcgggaccta cgcctttatt ttaacacgga cgccagcgct
     9301 tctactcccc gctccaccac atcgggcggc gccgcggtga agcagagccg gatgtgggtc
     9361 gggtaggggc cgaagctggg gcccggcgcg acgaagaggc cgcggtcgac gcagtcctcc
     9421 aggagcccct ggagcccgcg gtggtcgagg cggtccgcca cgtcgaggaa gaggaaggtg
     9481 ctgccctggg gcggctcgat gccgaggcgc gccgccgcct tgaggccggc ctgccggtag
     9541 gactgccggg cctccgcgat ccacggctcg cccgggccgg agagcacccg tagcgccgcg
     9601 atctgcgccg ccgtcggcgt gctgtagaag ctgtgcaggc tgaccttgcg cagctccttc
     9661 atcgccgccg ccggtcccac cacgtagccg cagcggttgc cggccatgcc gtaggccttg
     9721 gagaaggagt gggcggcgaa ggtgcgctcc ggagcgagcg gccgggtgta ggcgtgctcc
     9781 ccctcgaata ggtagtcctc gtagacctcg tccgagatga cccagaggtc ctcgctccgt
     9841 gcccattcca ccagcgcctc gatccacgcc cgggggatga tccggcccgt cgggttgctc
     9901 ggggtgttga ggtagagggc caccgtgcgg tcggtccgca gctcgcggac ggcctcgacc
     9961 gccgtctccg gggagtcggc ccggccgaag aaggggacgt ccaccggcac gccgtggaag
    10021 gactggatga tgccggtgat cagcggccaa tgaggcgcca gttggagcac ctcgtcgccc
    10081 ggggcgagga tcgcccccgc cacggcgccc aaggcgccgg tggcgccggt ggccaccagc
    10141 acgttctccc gggcagtcgg cgcgccggag cgggcctgga cccgctcggc gatcgcgtcg
    10201 aggagcgccg gcagcccctg gggcggcgcg tagcggtgca tgcccgggta gtccgacacc
    10261 cgcaggtcct ccatccgaca ccccgcgggg ggctccatcc aggtgtcccc cacgtgcagg
    10321 gggtagacct cgcccttgta gctctccagg cgatgagcca gggaggagta gacggctccg
    10381 gcgatgtggt cgaccgacgg ggagtatccg ggatagcgcg gcatggtccg cagatcctac
    10441 c
//
LOCUS       DASPJI010000560        11370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_36279, whole genome shotgun sequence.
ACCESSION   DASPJI010000560 DASPJI010000000
VERSION     DASPJI010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11370)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11370
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36279"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..345
                     /locus_tag="VEL74_15510"
     CDS             142..345
                     /locus_tag="VEL74_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63982.1"
                     /translation="MKRAVLLVVLVLSLVGVSAATVPQAEAWSCPPGVPYCTLGNNAQ
                     CDSWCGGPGWGSCVRPGCCSCLA"
     gene            complement(397..1308)
                     /gene="rarD"
                     /locus_tag="VEL74_15515"
     CDS             complement(397..1308)
                     /gene="rarD"
                     /locus_tag="VEL74_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HYG63983.1"
                     /translation="MSEPLPLPDAPPSAAEEVRKGALYAIAAYLWWGFSVFYFKAVAG
                     VPPLEIMAHRVVWSVPLLFAWLAWRGRLADVRRAVSDRRTLAILLVTTALIAGNWLVF
                     IIAVNSGRVTQASLGYYINPLVNVLLGVVFLGERLRGMQIAGVLLATAGVAFLTVSYG
                     ELPVISLFLAGTFALYGLLRKTVRADGMTGLAVETSLLLPLALGFLLVQEARGGLAFV
                     HGTWQETLLLPLGGLVTTLPLVWFANAVRRLRLATIGFIQYLSPSLQLLLATAVFGEP
                     FTRVHLVTFACIWLALALYSLDAWRRR"
     gene            1360..2250
                     /locus_tag="VEL74_15520"
     CDS             1360..2250
                     /locus_tag="VEL74_15520"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013334403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG63984.1"
                     /translation="MWKSLARRLARRRLGPSSGSAVPSGSAWPAWVAYEPDLVPPPAL
                     MGEEGISVLEEWFRWAEEWSMLLRVYGRVTAGSSVLEIGCGAGRIAFPLRYVLTRGSY
                     DGFDIRREPIEFLQRTFQAAHPTFRFHWADIHNTFYNPRGKLTTPQYRVPAADASKDI
                     VFAASIFTHMLPENTLHYFRESARVLRSGGRCLFSFFLLDHYRPGHPRPLGFAKPDFD
                     FGHPHGSHGDDFAIAFPKNPERMTAYRLALLRRFAAETGLELDGDPVPGLWSGTIESP
                     VGAQDVLVLVKLPRRSCEGS"
     gene            complement(2247..2705)
                     /locus_tag="VEL74_15525"
     CDS             complement(2247..2705)
                     /locus_tag="VEL74_15525"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HYG63985.1"
                     /translation="MSPDKIREAVVASKVDLIERMLAGIATLPLTTETDLTSDPRMVA
                     AGESFLRRALEALLDLGRHVLAKGFGIPAAEYKAVPRQLEQMEVLTPDLAARLLQMAG
                     YRNRLVHMYDEVTPAELYLILTHHLGDVPAVLHALRHWLAAHPERVDRAL"
     gene            complement(2702..3238)
                     /locus_tag="VEL74_15530"
     CDS             complement(2702..3238)
                     /locus_tag="VEL74_15530"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYG63986.1"
                     /translation="MDPVPGRGLPDGKARRRARGPFLVPLAPLRYDGSMSHPDPAALA
                     AVAKACREIVAGEPRIMAVYLFGSQATGEARPDSDVDLAVLFTEPVGLADLVALETRF
                     EERLDGPVDLINVGTCDPFLALEAIRGERLYCADPVRCDEFDLYVLRRAGDLEPFERE
                     RRAMLLGVRPGEARRRAS"
     gene            3997..4344
                     /locus_tag="VEL74_15535"
     CDS             3997..4344
                     /locus_tag="VEL74_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63987.1"
                     /translation="MPDYGNGIPSSAPTFAHIDLTQVTESTRRALYDAVVEVHFRPTP
                     AEVQKAAEDPDNHWIPDYGPDAAGLQVVHFKGRWFAIWRSLEEGDGAPEAHRWTVLRV
                     GRGEGGGLEFFEV"
     gene            complement(4416..5552)
                     /locus_tag="VEL74_15540"
     CDS             complement(4416..5552)
                     /locus_tag="VEL74_15540"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HYG63988.1"
                     /translation="MRKALIGLACLITTQTALVSAQVVPTTKPTCLFPASRTIACNQS
                     NTGTLEFNDCNYSSNFQSYDYDFWTFTGGAGSYVAIYAETSDSFFNPRVAAYGPDGSL
                     AGVGKDHLFDSSTDLLTGVLNANGTWTVMITSENPIIGSKEYYLEVQCSAVPPPQPPA
                     SPTQLAASTRSSTSIELSWQDNASNEDEYRIEVKTGESLFVDIGGVPTNTTSVNVTQL
                     VPGTSYTFRVRARRSSAYSSYSNEATATTTPETAACVSSETVLCLDDTPGDKRFEVRV
                     SYQTSQGGGLSGSGRAIPLSSLGVSQGGLFWFFSASNPELLVKVLNGCGSGGRYWVFF
                     SAGTNVGFSLTVRDAHTGIVKTYTNPDRTPAAPVQDTNSPFVCD"
     gene            complement(5891..6217)
                     /locus_tag="VEL74_15545"
     CDS             complement(5891..6217)
                     /locus_tag="VEL74_15545"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HYG63989.1"
                     /translation="MAKRTTAMRPTEARDFVEARLYETEEEVIRDALRHLLRARPEAQ
                     IQLAVHRYQTEELSLTKAADLAGVSWAQMREILLEKGISPKLGPETVEEAEAEVQALR
                     NFLARP"
     gene            complement(6436..6762)
                     /locus_tag="VEL74_15550"
     CDS             complement(6436..6762)
                     /locus_tag="VEL74_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019596003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor family
                     protein"
                     /protein_id="HYG63990.1"
                     /translation="MKKLIPCLFAILALSLLAPPVLADDAPATPAVQTGAAPEPLFLQ
                     GPPAGFPGQGNVCRIPPPFQPEECICILIFDPVCGCDNRTYSNACFARCEVFSFTPGA
                     CEGDEA"
     gene            6994..7191
                     /locus_tag="VEL74_15555"
     CDS             6994..7191
                     /locus_tag="VEL74_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63991.1"
                     /translation="MKRFVLLCLLVLSLLAVNVTAPNTASAAYECPWYTTYCWNSPQC
                     DSYCGGVGYGSCIRGCCACLA"
     gene            complement(7247..8170)
                     /locus_tag="VEL74_15560"
     CDS             complement(7247..8170)
                     /locus_tag="VEL74_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63992.1"
                     /translation="MLRSDTGPILVAGGDRDPNLRALVETLESRGEDVFFLQVGADHN
                     PWIAWDLASDRLTVDGEEVRPRAAFLRHDVFTNLADGRRESAYRAAAWHTAVYGWVLA
                     HEDVRMLNRWNRFPAANKPHALHLARQCGLAIPTTLITNHLGRLDAFEADRAKIVKPI
                     NGGAHTQRLHEVVGNTQNRGGVAAAPAIVQTELVPPEVRVFRVGDSFLAFKVLSEELD
                     YRSTQQTRVEPIPVPAGLAQGLGMLTDALGMDFGAADFKTCAETGRPLFLEINTSPMF
                     AAFNAAGRGAVTGAMADYLTGRPALRVTVAA"
     gene            complement(8173..8469)
                     /locus_tag="VEL74_15565"
     CDS             complement(8173..8469)
                     /locus_tag="VEL74_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63993.1"
                     /translation="MSNSNDTYRAGDVTTLAIGEEDPITTLAIGEEDVVTSLRVGEEE
                     GFNAGGYTTLSLGEEEPVTTLAIGEEDPYTTMAVGEEDKVSLESFNAGSPFGAF"
     gene            complement(8628..9266)
                     /locus_tag="VEL74_15570"
     CDS             complement(8628..9266)
                     /locus_tag="VEL74_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63994.1"
                     /translation="MLFTRPTVQARPFPPGLLEWQVSLRAHTAHERNGAPHAGVAPLL
                     TVRRPGVPLGVSSHSIICGILPHPSRLEAKTAEFRQIYEAAAPLGSREVYDRGLAYLR
                     DYYRNSEDFDHASLSTLLGKDSPAVQALRADGRCALVFYVFDLQDRSEVGRFRCLQVN
                     CEAEVLERGPVFDNVWWHNTLFHGKAEEHVVIHFRHQSTYDTRFGSFETLVD"
     gene            complement(9294..10109)
                     /locus_tag="VEL74_15575"
     CDS             complement(9294..10109)
                     /locus_tag="VEL74_15575"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG63995.1"
                     /translation="MTEPLQPLLTARGLHLRHPRSDRDAVRDLHLELRQGEILVILGP
                     NGSGKSTALAGLARGLRPRAGDVSLGAHPAWGLSRRAFARRVARLPQEPACPDGLTVE
                     ELVTGGRHPYANLLGDLRPADRAAVREALRWLDLEDLRGRAVARLSGGERRRAWLAMT
                     LCQGADVLLLDEPTASLDLKHQCEVLELLARINRERGVTLGIVLHDVDQAARLAHRVA
                     ILHRGRLYRAGPPRDCLTREMFLDVYGVETRITDEAGALRVQVLGPGDPTRSL"
     gene            complement(10106..11128)
                     /locus_tag="VEL74_15580"
     CDS             complement(10106..11128)
                     /locus_tag="VEL74_15580"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HYG63996.1"
                     /translation="MEAALSNLALVWDGWRRRPGFWAAIWTGILLAAAALSLTRGAVH
                     VPLGELGAALLQPAHPSHAVVWDIRLPRILAAGLTGAALAVAGALLQTVVRNPLADSG
                     LLGVNAGAGVAALFGLIFLPAAVAALPFLAFAGGLAAVALVLAVSWAGSQTASPLRIV
                     LSGVAVQAILFAAIGVLTFVFADRAPAFVSFTVGSLNGTGWREVRMATGPVALGLVLA
                     WTVTRPLDLLLLDDDSAGGVGLAVRRARVGASCLAALLAAAAVSVAGLVGFVGLVVPN
                     WIRIATGPAHRTLIPLSALGGAALLVLADLAARTLAAPVELPVGAFLAFLGGPYFLVL
                     LWRKLP"
     gene            complement(11128..>11370)
                     /locus_tag="VEL74_15585"
     CDS             complement(11128..>11370)
                     /locus_tag="VEL74_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63997.1"
                     /translation="LEVNDEVMASLRPELVLLATHGDPEEIRKAFLRKTAEGGPWRGM
                     SNVRQGIHVLDPALFATNPGLRMVEAARRLTGFAGR"
BASE COUNT         1852 a         3710 c         4053 g         1755 t
ORIGIN      
        1 ccgacgccgt gctcctggac gaggccaagg tcgggatcct cgccgggtcg ttcgtgtcgg
       61 cgattgccgg cagcctggtg ctcgtgcgga tggcgggcag gcaccactct tcatgaacga
      121 cctttcccga aaggagaatc catgaagaga gccgtcctcc tcgtcgtcct cgtcctgtcc
      181 ctggtggggg tgagcgccgc caccgtgccg caggcggagg cctggtcctg cccccccggc
      241 gtgccctact gcaccctcgg caacaacgcg cagtgcgaca gctggtgcgg cggacccggc
      301 tggggttcct gcgtgcgtcc cggctgctgc tcctgcctcg cctagccgtt tgtatggatg
      361 cccgccgccg atcctggacg gcgggctcct tgcgagtcag cgccggcgcc aggcgtccag
      421 gctgtagagg gcgagggcga gccagatgca ggcgaacgtc accaggtgaa cgcgggtgaa
      481 cggctcgccg aagaccgcgg tggcgaggag gagctgcagg ctcggcgaga ggtactggat
      541 gaagccgatg gtggcgagcc gcagccgccg gacggcgttg gcgaaccaga ccagcggcag
      601 ggtggtcacc aagccgccca ggggcaggag cagggtctcc tgccaggtgc cgtgcacgaa
      661 ggcgagcccg ccccgcgcct cctggacgag caggaatccc agcgccagcg gcagcagcag
      721 cgacgtctcg accgccaggc cggtcatccc gtcggcgcgg acggtcttgc gcagcagccc
      781 gtagagcgcg aaggtgccgg cgaggaacag cgagatcacc ggcagctcgc cataggagac
      841 ggtcaggaag gccacccccg ccgtggccag cagcacgccg gcgatctgca tcccccgcag
      901 gcgctcgccg aggaagacga cccccaggag cacgttgacc agcgggttga tgtaataccc
      961 caggctcgcc tgcgtcaccc gtccggagtt gaccgcgatg atgaagacga gccagttgcc
     1021 ggcgatgagg gccgtcgtga ccagcaggat ggcgagggtc cggcggtcgg agacggcgcg
     1081 gcggacgtcg gccaggcgcc cgcgccacgc cagccacgcg aagagcagcg gcacggacca
     1141 gacgacgcgg tgcgccatga tctcgagcgg cggcaccccg gcgaccgcct tgaagtagaa
     1201 gacggagaac ccccaccaca gataggcggc gatagcgtag agagccccct tccggacctc
     1261 ttcggccgcg ctgggtggag catcggggag cgggaggggc tcggacatcg gcgcagtcta
     1321 tccgcacggc acgccctctg cggatagact gttcccgtga tgtggaaatc ccttgccagg
     1381 aggctggccc gccggcgcct ggggccatca tccgggtcgg cggtgccgtc cggatcggcc
     1441 tggcccgcgt gggtggccta cgagccggac ctcgtcccgc cgccggccct catgggggag
     1501 gaggggatct cggtgctgga ggagtggttc cgctgggccg aggagtggag catgctgctc
     1561 cgcgtctacg gccgggtgac cgccggcagc tccgtcctcg aaatcggctg cggcgccggc
     1621 cggatcgcct tcccgctgcg ctacgtcctc acccggggga gctacgacgg cttcgacatc
     1681 cgccgggagc cgatcgagtt cctgcagcgc accttccagg cggcccatcc gaccttccgc
     1741 ttccactggg cggatatcca caacaccttc tacaacccgc gggggaagct gaccacgccg
     1801 cagtaccggg tgccggctgc ggacgcctcg aaggacatcg tcttcgccgc ctcgatcttc
     1861 acccacatgc tgccggagaa cacgctgcac tacttccggg agtcggcccg cgtcctccgc
     1921 tccgggggcc gctgcctctt cagcttcttc ctcctggacc actaccgtcc cggccacccc
     1981 cgccccctcg gcttcgccaa gcccgacttc gacttcggcc atccccacgg cagtcacggg
     2041 gacgacttcg ccatcgcctt ccccaaaaac cccgagcgga tgaccgccta ccgtctcgcc
     2101 ctgctgcggc gcttcgccgc cgagaccggc ctggagctgg acggcgatcc cgtgccgggg
     2161 ctctggtccg gcacgatcga gtcgccggtg ggggcgcagg acgtgctggt gctggtgaag
     2221 ctcccaagac ggagctgtga ggggtcctag agcgctcggt ccacgcgctc cgggtgagcc
     2281 gcgagccagt ggcgcagggc atggagaacg gccggcacgt cgccgaggtg atgggtgagg
     2341 atgaggtaga gctcggcggg ggtgacctcg tcgtacatgt ggacgagccg gttccggtat
     2401 ccggccatct gcaggaggcg agcggcgagg tcaggcgtca gcacttccat ctgctcgagc
     2461 tgacgcggga cggccttgta ctcggcggcg gggatgccga accccttggc cagcacgtga
     2521 cggcccaggt cgagcaaggc ctccaaggcc cggcggagaa aggactcgcc tgcggccacc
     2581 atgcgagggt ccgaggtgag gtccgtctcg gtggtcagag gaagcgtggc aatccccgcg
     2641 agcatgcgtt cgatcagatc gaccttggag gcgaccaccg cttcgcggat cttgtccggg
     2701 ctcatgacgc tcgccgtcgc gcctctccgg gccgcacgcc cagcagcatc gcccggcgct
     2761 cgcgctcgaa cggctccagg tcaccggccc ggcgcaggac atagagatcg aactcgtcac
     2821 aacgcaccgg gtcggcgcag tagaggcgct cgccccggat cgcctcgaga gcgaggaagg
     2881 ggtcgcaggt gccgacgttg atcaggtcga ccgggccgtc gagccgctcc tcgaaacgcg
     2941 tctccagggc cacgaggtcc gcgagcccga ccggctcggt gaacagcacc gccaggtcca
     3001 cgtcggaatc cggccgcgcc tctccggtag cctgggagcc gaagagatag acggccatga
     3061 tccgtggttc gcccgcgact atctccctac atgccttggc aacggccgcg agcgcagctg
     3121 gatcagggtg gctcatggag ccatcataac ggaggggcgc cagaggcacc agaaacggtc
     3181 cacgggcacg gcgccgcgct ttcccatccg gcaaaccgcg cccgggaacc gggtccatcg
     3241 attgtcaaag aaccttccta gcctttcccg cgcggtctga cccgcgccgt actcgtttcg
     3301 accctctccg gagctcccgc gacccgaggg tcgagaggct ccgcaacgtt cgcgagcgcc
     3361 tcgacccgag tcaaacctcg cgcgaccctg gggtccaacc tcgcgaccct cgggtcgaga
     3421 ggctcgaccc gagggtcgga accactttgt ccagggtcga accctctgac ccaggcgtgt
     3481 gttctcgcag cagccagggc gaatcagctc aaggaacgcc aggcaggcgc tcaaacgagg
     3541 cgttataaga tcgtatcggg ccatggatgt caattctggt cttgtaaccg gaccggactc
     3601 cgtgtagatt gggctggagg cggggccgtg gtccggagcc cgccgcgggg tgatctttga
     3661 caactgaaga gtacgggggc gccgccgtgt ccaaggccgc gcccccggcg tgggaggcgg
     3721 ggggagtcct ccgcctccgg tggccagggg tacgtcgtcc acccgcgcga tttgatcttg
     3781 acacactcgc tcaacccggc gtaagctatc cgcgcagtcg caagctgtac ccctatacat
     3841 cgggaaggga ggtgttgccg ggaggagacc tgacgcgaga ctcgacggag tgtcccagga
     3901 gagggtgtgc ctctcccggg agagatcgaa gactgagacg gccccggaag gagatagagg
     3961 ctccaaccgg ggcctcgccg agcaggagac cagctcatgc ccgactacgg gaatggtata
     4021 ccttcgtccg ccccgacgtt tgcccacatc gacctgactc aggtgaccga gtccacccgc
     4081 cgcgcgctgt acgacgcggt ggtcgaggtc cacttccgtc ccaccccggc cgaggtccag
     4141 aaagccgccg aggacccgga caaccactgg atccccgact acggtccgga cgccgccggc
     4201 ctgcaggtcg tccacttcaa gggccgctgg ttcgcgatct ggcggtccct cgaagagggc
     4261 gacggcgcgc cggaagcgca ccgctggacg gtgctccgcg tcgggcgggg ggaggggggc
     4321 gggttggagt tctttgaggt gtaggggcgg gaggtagtgg agtagcaggg taggtggccg
     4381 gggcggcggg tgggtgacgc cgccccggtc gagagctagt cacatacgaa ggggctgttt
     4441 gtgtcctgca caggggcggc cggcgttcta tccgggtttg tgtaagtctt cacgatacca
     4501 gtatgcgcat cccggactgt gagcgaaaac ccgacattcg tgccggctga gaagaagacc
     4561 cagtaccggc cgcctgagcc acatccattc aacaccttga cgagcaactc ggggttactc
     4621 gcagagaaga accaaaacaa tccaccctgg ctgacaccga gggaggatag ggggatcgct
     4681 ctcccggaac cgctcagtcc gccaccctgc gatgtttggt aagacaccct aacctcaaag
     4741 cgcttgtcac caggtgtgtc gtcgaggcag agtacggttt ccgaggaaac gcacgctgca
     4801 gtctcaggtg tagtcgtcgc ggtggcctca ttggagtagg acgagtacgc acttgagcgc
     4861 cgagctctga cgcgaaaagt atatgaggtt cctggaacaa gctgcgtaac atttaccgaa
     4921 gttgtattgg tcgggacacc gccgatgtcg acaaatagcg actcgcctgt cttgacttct
     4981 atcctgtact cgtcctcatt ggaggcatta tcctgccatg agagctcgat cgaagtgcta
     5041 gaccgcgttg aagctgcaag ctgagtggga gacgcagggg gctgtggtgg aggcacggca
     5101 gagcattgaa cttcaaggta gtattccttg ctgccaatta tgggattttc ggaggttatc
     5161 atgacagtcc atgttccgtt ggcattcaga acgcctgtta ggagatctgt gctggagtcg
     5221 aataaatggt cctttccgac ccctgctaga gacccgtctg gcccgtatgc cgctactctc
     5281 gggttaaaga atgagtccga ggtctccgcg tagatagcta cgtacgaacc cgctccgccg
     5341 gtgaaggtcc agaagtcata atcgtagctc tggaaattgg atgagtaatt acaatcattg
     5401 aactctagag ttcccgtgtt gctttggttg caagcaatcg tcctcgaggc tgggaacagg
     5461 caggtgggct tggttgtggg caccacctgt gccgagacca acgccgtctg tgttgtaatc
     5521 aaacacgcca ggccaatcaa cgctttgcgc atgctgtctc tccttgagtc tgtttggcga
     5581 ccgagggctg gatcaatctt ggtagatgtt gagacgtccg attcgtgcta acgacgatga
     5641 acgccccggc ataggcataa agccaggtag ccgtaatagt cacctctcaa gccccagagg
     5701 ggcggaggca gttcggttcc ggaacgatcg ttcagcgctc atgagttgca tgcctgcgcc
     5761 tcttcgggcc gaagaatggt ggggggcggc gtgacccagg gttgaagcca tgggctttat
     5821 gcctgtgccg cttcgcggcg ttggcgctga gcagaagcct gttggaggac tgtcctgcga
     5881 gggatgacgc tcatggtcta gccaggaagt tccgaagagc ctgtacctcc gcctcggcct
     5941 cttctacggt ttcgggcccg agcttggggg agatgccctt ttcgaggaga atctctcgca
     6001 tctgcgccca actcactccg gcgaggtcag ccgccttggt caaggagagc tcttcggtct
     6061 ggtaccggtg gaccgcaagt tgaatttgcg cctcggggcg ggcgcgaagt aagtggcgga
     6121 gcgcatcgcg gatcacctcc tcctcggtct cgtagagccg tgcctcgacg aagtcccggg
     6181 cttcggtagg tctcatcgcg gtcgtccttt tggccatgct cctctccttc cctgagatcc
     6241 taccatcgtg gaggaggagc aaccggcgaa gcctggcccc ccgacgcggt gggctggagg
     6301 gttggatgac ggcgtgaccc agggttgaaa ccctgggctt tatgcctgtg ccgcttcgcg
     6361 gcgtttggag gggagttgat atcgaccagc gaggcccgcc ccgcccgggg gcgggcctcc
     6421 tctcgtccgg gatggctagg cctcgtcgcc ttcgcaggct cccggggtga agctgaatac
     6481 ctcacagcgg gcgaagcagg cgttcgagta ggtgcgattg tcgcagccgc agaccggatc
     6541 gaagatcagg atgcagatgc actcctccgg ctggaagggg ggcgggatgc ggcagacgtt
     6601 gccctgcccg gggaatccgg ccggcggccc ctgcaggaag aggggctccg gcgcggcgcc
     6661 ggtctggacg gcaggggtcg cgggcgcatc gtccgccagg acaggggggg cgaggagcga
     6721 cagggccagg atcgcgaaga gacacgggat gagcttcttc atggcaggac ctccttcgga
     6781 ctgcggatca ggtggggggg ccagggcagc cgcccccggg gtgcacggcc ggctacacgc
     6841 gcggccagtc ttccgagact agcactttag gaatccgatg tggagtgctt gcgcggaatc
     6901 tgtcccgcgc tcgccttgcc gctcctcccg cacctctctc tatgagggac ccatcgagcg
     6961 taggtgctgg tcccattcaa tgaaaggaac gatatgaaga gatttgtcct gctctgcctc
     7021 ttggtcctgt cgctcctcgc ggtcaacgtc accgcgccca atacggcgtc cgctgcctat
     7081 gagtgcccgt ggtacacgac gtactgctgg aactcgccgc agtgcgacag ctactgcggt
     7141 ggcgtcggct acggctcctg catccgcggc tgctgcgcct gcctcgcgta gagcgacccc
     7201 gcccgacctc gcggtcaacc tcgcggcctg gcgggggcct ctcccgtcag gccgcgacgg
     7261 tcacccgtag cgccggccgt cccgtcaggt agtccgccat ggcaccggtg acggctcccc
     7321 gtccggcggc gttgaaggcc gcgaacatgg gggaggtgtt gatctccagg aagaggggac
     7381 ggcccgtctc ggcgcaggtc ttgaagtccg cggcgccgaa gtccatcccc agggcatccg
     7441 tcagcatccc cagcccctgg gcgaggccgg ccgggaccgg gatgggctcc acccgcgtct
     7501 gctgcgtcga gcgatagtcg agctcctctg aaagcacctt gaaggccagg aaagagtcgc
     7561 cgacccggaa gacccggacc tccggcggca ccagctcggt ctgcacgatg gccggcgccg
     7621 ccgccacacc accgcggttc tgagtgttgc ccactacttc gtgcaggcgc tgggtgtggg
     7681 cgccgccgtt gatcggcttg acgatcttgg cgcggtccgc ctcgaacgcg tccagccgcc
     7741 cgaggtggtt ggtgatcagg gtggtgggga tcgcgaggcc gcactgccgc gccagatgca
     7801 gggcgtgcgg cttgttggcc gccgggaagc ggttccaacg gttgagcatg cggacgtcct
     7861 cgtgcgccag gacccagccg tacacggccg tgtgccaggc cgcggcacgg taggccgact
     7921 cgcggcggcc gtccgccagg ttggtgaaga cgtcgtggcg gaggaaggcc gcccgcgggc
     7981 ggacctcctc gccgtcgacg gtcaggcggt cggacgccag gtcccaggcg atccaggggt
     8041 tgtgatcggc tccgacctgg aggaagaaga cgtcctcccc ccgggattcg agcgtctcga
     8101 cgagcgcccg caggttcggg tcgcggtcgc ctcccgcgac gaggatgggc ccagtgtccg
     8161 accgcagcat gatcagaacg ctccgaaggg gctgccggcg ttgaaggact cgaggctgac
     8221 cttgtcctcc tcgcccacgg ccatggtggt gtaggggtcc tcttcgccga tggcgagggt
     8281 ggtcaccggc tcctcctcgc cgagcgagag ggtggtgtag ccgccggcgt tgaagccctc
     8341 ctcctcgccg acgcgcaggg aggtcacgac gtcctcctcg ccgatcgcga gggtggtgat
     8401 ggggtcctct tcgccgatcg cgagggtggt gacgtcgccg gcgcggtacg tatcgttcga
     8461 gttgctcatg atcagatctc ctcaggtttc agggtttcta tctatataga agcgggctgt
     8521 cccctcggag cctgcgaggt ctctcgtgcg tttccgagag gggctcaggc gcccgcgttg
     8581 tagagatcaa cgatggagcg cgggcgatcc cctcactttt cggcggctca atccaccagg
     8641 gtttcgaagc ttccgaagcg ggtgtcgtag gtgctctggt gccggaaatg gatcaccacg
     8701 tgctcctcgg ccttgccatg gaaaagggtg ttgtgccacc agacgttgtc gaacaccggc
     8761 ccccgttcca gcacctcggc ctcgcagttc acctgcaagc agcggaagcg cccgacctcg
     8821 ctgcggtcct ggaggtcgaa gacgtagaag acgagggcgc agcggccgtc cgcccgcagc
     8881 gcctggacgg ccgggctgtc cttgcccagg agggtgctga ggctcgcgtg atcgaagtcc
     8941 tccgagttcc ggtagtagtc ccgcaggtag gcgaggccgc ggtcgtagac ctcgcgcgac
     9001 cccagcggcg ccgccgcctc gtagatctgc cggaactcgg cggtcttcgc ctccagccgc
     9061 gaggggtggg ggaggatgcc gcagatgatc gaatggctgc tcacccccag gggcacgccg
     9121 ggccggcgca ccgtgagcag cggcgccacc ccggcgtgcg gcgcgccgtt cctctcatgg
     9181 gcggtgtggg cgcgcaggct cacctgccac tccagcaacc ccggagggaa ggggcgggcc
     9241 tgcacggtcg gacgcgtgaa gagcatggga tctcctttcc aatccaataa atgtcagagg
     9301 ctccgcgtcg ggtctcccgg gccgaggacc tggacacgca gggcgccggc ctcgtcggtg
     9361 atccgggtct cgacgccgta gacgtccagg aacatctcgc gggtgaggca gtcgcggggc
     9421 ggtccggcgc ggtagaggcg gccgcggtgg aggatggcca cgcgatgggc gagccgggcg
     9481 gcctggtcga cgtcgtggag gacgatgccg agggtgacgc cgcgctcccg gttgatgcgg
     9541 gcgagcagct ccagcacctc gcactggtgc ttgaggtcga ggctcgcggt gggctcgtcg
     9601 aggaggagga cgtccgcccc ctggcagagg gtcatcgcca gccaggcgcg gcggcgctcg
     9661 ccgccggaga gacgggcgac ggcgcggccc cgcaggtctt cgaggtcgag ccagcgcagg
     9721 gcctcgcgca cggcggcgcg gtcggcggga cgcaggtcgc cgagcaggtt ggcgtagggg
     9781 tgacggccgc cggtgaccag ctcttcgacg gtgaggccgt cagggcaggc cggctcctgg
     9841 ggaaggcggg caacgcgccg cgcgaaggcc cggcgggaga gcccccaggc cggatgcgcg
     9901 ccgagggaga catcgccggc gcggggccgg aggccgcggg cgaggcccgc cagcgccgtc
     9961 gacttgccgg agccgttggg gccgaggatg acgaggatct cgccctgccg cagctcgagg
    10021 tggaggtcgc ggacggcgtc gcggtcgctg cgcgggtgcc gcagatggag gccgcgggcc
    10081 gtcagcagcg gttggagcgg ttcggtcacg gcagcttcct ccagaggagc acgaggaaat
    10141 aggggccgcc caggaaggcg aggaaggcgc cgaccggcag ctcgaccggc gcggcgaggg
    10201 tgcgggcggc gaggtccgcc agcaccagca gcgccgcgcc gcccagggcg gagagcggga
    10261 tcagcgtgcg atgagccggg ccggtggcga tgcggatcca gttcggcacc accaggccga
    10321 cgaagcccac gaggccggcg acgctcaccg ccgccgcggc gagcagggcc gccaggcacg
    10381 aggcgccgac ccgggcgcgg cggaccgcga ggccgacgcc gcccgccgag tcgtcgtcca
    10441 gcagcaggag gtcgaggggg cgggtcaccg tccaggccag gacgaggccg agggcgacgg
    10501 ggccggtggc catccgcacc tcgcgccagc cggtgccgtt gagcgatccc acggtgaagc
    10561 tgacgaaggc cggcgcgcgg tcggcgaaga cgaaggtgag cacgccgatg gccgcgaaga
    10621 ggatcgcctg caccgcgacc ccggagagga cgatgcgcag cgggctcgcc gtctggctcc
    10681 ccgcccagga gacggcgagg acgagggcga cggcggcgag cccgccggcg aaggcgagga
    10741 aggggagggc ggcgacggcc gcgggcagga agatcagccc gaagagcgcc gccaccccgg
    10801 cgccggcgtt cacccccagg aggcccgagt ccgccagggg gttgcgcacc accgtctgga
    10861 ggagcgcgcc cgccaccgcc agggcggcgc cggtgagccc cgcggccagg atgcgcggca
    10921 gccggatgtc ccagaccacc gcgtgcgacg ggtgcgccgg ctggagcagg gcggcgccga
    10981 gctctcccag aggcacgtgc accgcgcccc gggtgaggga gagcgccgcc gccgccagga
    11041 ggatgccggt ccagatcgcg gcccagaacc ccggccgccg gcgccagccg tcccagacca
    11101 gcgcgaggtt ggagagggcc gcttccatca gcgccccgcg aacccggtga gccggcgcgc
    11161 cgcctccacc atccggagcc ccgggttggt ggcgaagagc gccggatcga ggacgtggat
    11221 gccctggcgg acgttggaca tcccccgcca gggccccccc tccgcggtct tgcggaggaa
    11281 cgccttgcgg atctcctccg gatcgccgtg ggtcgccagg aggacgagct cgggccgcag
    11341 cgacgccatc acctcgtcgt tgacctccag
//
LOCUS       DASPJI010000561         4905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_93562, whole genome shotgun sequence.
ACCESSION   DASPJI010000561 DASPJI010000000
VERSION     DASPJI010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93562"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="VEL74_15590"
     CDS             <1..1298
                     /locus_tag="VEL74_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG63998.1"
                     /translation="LAGGPELAHLTSGDSDWYDWLFDTLTQAGDKLDFVTHHVYDGDG
                     PEDVAEKLDANTLFGGRPSFWGTVAPSVREVLEEAGATDKPFWLTEIGWETANVSEAR
                     QAQNYTGLLTDWFTGRAGRDWVDKVFFYEMKDGGAADSPTWGILRPDGSPKPAWGAYR
                     DFTASWAGSQGRTLALLGGRFKVEVSWRDHQGKTGTGHAVPDSDQSGFFWFFDQANLE
                     LVVKALDGRALNGRWWIFYGALSDVEYWLTVTDTQTGQVRRYHNPPGNLCGKGDTSAF
                     PLPAAASSGVAADESFEAVPFSGTAVLEADHLAAGTGLVTAAAGSCGGGANALCLLGS
                     RFRVEVDWRRRDGVTGVGTPVPRTDQSGTFWFFDPANLELVVKILDGRALTGRFWVFY
                     GALSDVQYRITVTDTVTGASKVYQNPAGNLCGRGDTQAL"
     gene            1304..1648
                     /locus_tag="VEL74_15595"
     CDS             1304..1648
                     /locus_tag="VEL74_15595"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HYG63999.1"
                     /translation="MPFFKVGKEAAFTPGSLHAVTLLGRRIGIVRDPDGTLRAMEVTC
                     RHQNADLTQGQRDGDVVTCPRHGWRYDLRTGECLTEPWARLRSFDLQVVDGEVLVSPS
                     PREEPREREEGG"
     gene            2288..2638
                     /locus_tag="VEL74_15600"
     CDS             2288..2638
                     /locus_tag="VEL74_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64000.1"
                     /translation="MPSFGDRTPSGVDLSRPTESTRRHLYELVVRVYFQPTPEEVEQA
                     RQDPGNHWVPDYGPDEARLHVFFVYGRWFAVWEALEEKKETGPLPEWRRWSVVRIVED
                     ADPSSATGVTFLEV"
     gene            complement(3160..3411)
                     /locus_tag="VEL74_15605"
     CDS             complement(3160..3411)
                     /locus_tag="VEL74_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HYG64001.1"
                     /translation="MITIRDKETGRELGQVTPEQFQALVDVLEEESRDDNDYYINRTT
                     LGLLEEAGADPNLLALLDQGLAGREEMDIQWRREGSEIS"
     gene            3541..3951
                     /locus_tag="VEL74_15610"
     CDS             3541..3951
                     /locus_tag="VEL74_15610"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG64002.1"
                     /translation="MKNSMRLLGMTNRDIERQKGWSSSYLSRLFSGAIELKYEHILDI
                     CEVMGLEPAEFFQMAYGSPDQAPPSEAALKLRKSIQIFMPARTGAAPAPPAAQAAEGS
                     TEPRQEKEIDEELMERMLAKTLRRLLQDTQKAGE"
     gene            4087..>4905
                     /locus_tag="VEL74_15615"
     CDS             4087..>4905
                     /locus_tag="VEL74_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYG64003.1"
                     /translation="MSEQYIYVMKDLRKIYPPNREVLKGIWLSFFPGAKIGVLGLNGS
                     GKSTLLKIMAGEVKDFGGEAWAAKGARVGYLSQEPRLDPEKDVLGNVEEGVAETKALL
                     NRFDEINARFGEDLSPEEMDKLLAEQARVQDAIEAANAWELDRTLEIAMDALRLPPPD
                     AEVGKLSGGEKRRVALCRLLLQRPDLLLLDEPTNHLDAESVAWLERHLAEYAGTVVAV
                     THDRYFLDNVAGWILELDRGQGIPYEGNYTAWLEQKEKRLEQESREEGSRQRTIK"
BASE COUNT          778 a         1705 c         1686 g          736 t
ORIGIN      
        1 ccctggcggg ggggccggag ctggcccacc tcacctccgg cgacagcgac tggtacgact
       61 ggctgttcga caccctgacg caggccggcg acaagctcga cttcgtcacc caccacgtct
      121 acgacggcga cggcccggag gacgtggccg agaagctcga cgccaacacc ctcttcggcg
      181 gccggccgtc cttctggggc acggtggcgc cgtcggtgcg cgaggtgctg gaggaggcgg
      241 gcgccacgga caagccgttc tggctcaccg agatcggctg ggagaccgcc aacgtctccg
      301 aggcgcggca ggcgcagaac tacaccggcc tgctcaccga ctggttcacc ggccgcgccg
      361 gccgcgactg ggtcgacaag gtcttcttct atgagatgaa ggacggcggc gccgcggaca
      421 gcccgacctg ggggatcctg cggccggacg gcagcccgaa gccggcctgg ggcgcctatc
      481 gggacttcac cgcgtcctgg gccgggtcgc aggggcggac gctggcgctc ctcggcggcc
      541 gcttcaaggt cgaggtgagc tggcgcgacc accaggggaa gacgggcacc ggccacgccg
      601 tccccgactc cgaccagagc ggcttcttct ggttcttcga ccaggcgaac ctggagctgg
      661 tggtcaaggc gctggacggc cgggcgctca acggccgctg gtggatcttc tacggcgccc
      721 tctcggacgt cgagtactgg ctcaccgtca ccgacaccca gaccggccag gtccgccgct
      781 accacaaccc gccgggcaac ctctgcggca agggggacac ctcggccttc ccgctccccg
      841 cggcagcttc gagcggggtg gcggcggacg agtccttcga ggcggtgccg ttctccggca
      901 ccgccgtcct ggaggcggac cacctcgccg ccggcaccgg cctggtgacc gccgccgccg
      961 gctcctgcgg cggcggcgcc aacgccctct gcctcctcgg ctcgcgcttc cgggtcgagg
     1021 tcgactggcg gcggcgcgac ggcgtcaccg gcgtcggcac cccggtgccg cgcaccgacc
     1081 agagcggcac cttctggttc ttcgacccgg cgaacctgga gctggtggtg aagatcctgg
     1141 acggccgcgc cttgaccggc cgcttctggg tcttctacgg cgcgctgtcg gacgtgcagt
     1201 accggatcac cgtgaccgac accgtcaccg gcgccagcaa ggtgtaccag aacccggccg
     1261 gcaacctctg cggccgcggc gacacccagg ccctctgaag aggatgccgt tcttcaaggt
     1321 ggggaaggag gcggccttca cccccgggag tctccacgcc gtgacgctcc tgggccggag
     1381 gatcggcatc gtccgcgatc cggacggcac cctgcgcgcc atggaggtga cctgccgcca
     1441 tcagaacgcc gacctgaccc aggggcaacg cgacggcgac gtggtgacct gcccccgcca
     1501 cggctggcgc tacgacctgc ggaccggcga atgcctcacc gagccctggg cgcggctgcg
     1561 cagcttcgac ctgcaggtgg tggacggcga ggtgctggtc tcgccctccc cccgggaaga
     1621 gccgcgggag cgggaagagg ggggctgagg ccggacctca ccctccggcc tgggcaagcc
     1681 cccccgccgc ctgtccggaa gaattgttcg tcgcagagca ggattcccga ggctcgcgac
     1741 ccgggaagaa cgtcgcagag gcgcgttgat tcgtcgcaga tgagctccgc tctgcgacgt
     1801 ttgaacgcag ctctgcgacg aacgaacgtt catctgcgac gaacaagcgt tcatctgcga
     1861 tgattgaacg ctgctctgcg acgtttcagc gcaactctgc gacgatcgga tcgaagggag
     1921 gtggctcaga agccgccatg aaggggcggt ggtgcctccc cccctgggcg ggtgggcggg
     1981 agggagcggg ggaggtcagg aggggggttt ttcatcgccc ccgaaccctc gacttcccgc
     2041 aagcccccct cattttcatc ttgacgtacc cccggtcgcg ttatagtctc gggcggtcgt
     2101 aagccgtaca acagaaaagg aggtggcagg ggagagaagc aagccgcagc ttcacagcac
     2161 agccgtacag gatcgctgtg cctccaaggt gagcgcgtgt ctcatccgag gctccagggt
     2221 ccaagcggcc aagggaggga gcgaccaact cgcctcccga ggcctagccg gacaggagat
     2281 gcaactcatg cccagcttcg gtgatcgtac gccttcgggc gtcgatctgt cccgtcccac
     2341 cgaatccacc cgccgtcatc tctacgagct ggtggtccgc gtctacttcc aacccactcc
     2401 tgaggaggtg gagcaggccc gtcaggatcc cggcaaccac tgggtgccgg actacggtcc
     2461 cgacgaggcg cgcctgcacg tcttcttcgt ctacggccgc tggttcgcgg tctgggaagc
     2521 gctggaggag aagaaggaga ccgggccgct cccggagtgg cgccgctggt cggtggtgcg
     2581 gatcgtcgag gacgcggacc cgtcgagcgc gacgggcgtc acctttctgg aggtctgacg
     2641 ctcccggcgc gacatccgcc gcctcgcgcg gcccgcctat ctatagaagg aagcagcgcc
     2701 ttctccgccg tcgcggggga ggcgcgccga cgcccccgtt ttcagccgcg aagcggcgcg
     2761 ggcgtagagc ctggggtttc aacccaggta agcgcaaaat tccaaagtcc cgaagggacg
     2821 taggcagctc gacgggtggg gaggattgcc tgcgcccttt cagggctgga gggtgggggt
     2881 ggggcgccta cctggcattg aaatgccagg ctgtacgcct gcgccgcttc gcggctgatc
     2941 tggtcggcga ttgcgtgttg atgctggctg cgtacacgga ggcggcgctg caaaaccccc
     3001 ctcctgacct cccccgctcc ctcccgccca cccgcccagg gggggaggca ccaccgcccc
     3061 ctctccggcg gtccgtgtcc gtccgtgctc gtccatgttg ccccccgtcc gcctccccct
     3121 gtcccgggga ggcgggagag ggggccgggg ggtgagggcc tacgagatct ccgacccctc
     3181 gcgccgccac tggatgtcca tctcctcgcg gccggcgagc ccctgatcga gcagcgccaa
     3241 caggttggga tcggcaccgg cctcttccag caggccgagg gtggtgcggt tgatgtagta
     3301 gtcgttgtcg tcccgggact cctcctcgag gacgtccacc agcgcctgga actgctcggg
     3361 cgtcacctgc cccagctccc gtcccgtctc tttgtcgcgg atcgtgatca tgcctcctcc
     3421 ttcgtccgtg cggggcagaa cctatcacga ctcccgggga gcagagcgct cgttgcaacc
     3481 ggtcttccgg aactgtaaac tcggggcatg gtaccggaga tccgaagagt cctcgatctg
     3541 ttgaagaact cgatgcgcct gctcggcatg acgaaccgtg acatcgagcg ccagaagggt
     3601 tggagctcga gctacctcag ccggctcttc tcgggcgcca tcgagctcaa gtacgagcac
     3661 atcctggaca tctgcgaggt catgggcctg gagccggcgg agttcttcca gatggcctat
     3721 ggctcgccgg accaggcgcc gccgagcgag gcggcgctca agctccgcaa gtcgatccag
     3781 atcttcatgc cggcgcggac cggcgccgcg cccgctcccc cggcggcgca ggccgcggag
     3841 ggctccaccg agccgcggca ggagaaggag atcgacgagg agctgatgga gaggatgctg
     3901 gcgaagaccc tgcgccggct gctgcaggac acccagaagg ccggcgagta gccccggcgc
     3961 ggctcccccg ccctccctct cctccctcgg gccgccgcgc gcggggtggc ccgtccgggt
     4021 ggggtagaat ccaccggttc agtcctgtcg cctgtcccaa ccacaggatc gaggagtcgt
     4081 catcacatgt ccgaacagta catctatgtc atgaaggacc tgcggaagat ctatccgccg
     4141 aaccgcgagg tcttgaaagg gatctggctc tccttcttcc cgggagccaa gatcggcgtg
     4201 ctcggcctga acggctccgg caagagcacg ctgttgaaga tcatggccgg cgaggtcaag
     4261 gacttcggcg gcgaggcctg ggccgccaag ggggcgcgcg tcggctacct ctcccaggag
     4321 ccgcggctcg acccggagaa ggacgtcctc ggcaacgtcg aggagggggt ggccgagacc
     4381 aaggcgctgc tcaaccgctt cgacgagatc aacgcccgct tcggcgagga cctctccccc
     4441 gaggagatgg acaagctgct cgccgagcag gcgcgggtgc aggacgccat cgaggcggcc
     4501 aacgcctggg agctcgaccg cacgctcgag atcgccatgg acgccctgcg cctgccgccg
     4561 cccgatgccg aggtcggcaa gctgtcgggc ggcgagaagc gccgggtcgc gctctgccgg
     4621 ctgctcctcc agcgccccga cctgctgctg ctggacgagc ccaccaacca cctggacgcc
     4681 gagtcggtgg cctggctgga gcgccacctc gccgagtacg ccggcacggt ggtggcggtc
     4741 acccacgacc gctacttcct cgacaacgtg gcgggctgga tcctggagct cgaccgcggc
     4801 cagggcattc cctacgaggg caactacacc gcttggctgg agcagaagga aaagcggctc
     4861 gagcaggaat cccgggaaga gggctcgcgc cagcgcacca tcaag
//
LOCUS       DASPJI010000562        15989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_669192, whole genome shotgun sequence.
ACCESSION   DASPJI010000562 DASPJI010000000
VERSION     DASPJI010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15989)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15989
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669192"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VEL74_15620"
     CDS             complement(<1..706)
                     /locus_tag="VEL74_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64004.1"
                     /translation="MRTKAIFPLALLLSALVSSFVTPERALSQATRSRPPGPPRTARQ
                     PDQFDPPRQEERTSVSEMARRQVTRPAERQVDVTLWPEAQRSFWQDGPALLLTEAQRD
                     ELLSLDADGREQFIRTFLSQDPIPETRGNELTEAIERRRRLAAIEYMSPTDVRAQLLF
                     LNGPPAERKPIDCGNAFRPLEIWTWKGGIDEEGKPRERKAVVFRPSPEQPFQFWIPSD
                     SKRVLYAEEMENLLQQI"
     gene            829..1953
                     /locus_tag="VEL74_15625"
     CDS             829..1953
                     /locus_tag="VEL74_15625"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYG64005.1"
                     /translation="MSKSRRNAPIHSWEAGDNVQGFALLTKKEMRQDRNGKSFLDLEL
                     TDATGAIVAKVWADSPALHGQYETHSFIAFRGSVKSYRDQLQLTIDDCRCATEDDRRL
                     GFDEALLIPSTHEDIDDLWARLVRICEEEVDRPVLRRLAAETLELYKKELREHPAAKS
                     MHHAYRGGLLEHVVSMAELAVQVCRHYQDLDRDLILLGVLFHDLGKLQELGAMPANDY
                     TLEGRLIGHIVLGRDLLRERCAAIPDFPADLRLILEHLVLSHQGKKEFSSPVEPMTPE
                     ALALHFIDDLDSKLNQLRRARELSPGMQFHRGLGRFVYLPAPVEPSLDDTMVALPGRP
                     EEMEVGRTAPVPGAVVIEVTESGAPAQPEEAPQPRLDFGR"
     gene            2391..2855
                     /locus_tag="VEL74_15630"
     CDS             2391..2855
                     /locus_tag="VEL74_15630"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG64006.1"
                     /translation="MHLRIAYLSDHPEAISTLARWFQEESPDYFAGWAPEEMEQHFQP
                     WLHRDRLPLGLVAFERGEVVGCIVLREKALDREPAHSPGLGGLHVAEPHRGRGIGSAL
                     VKAGMDAARALGYDAVYTGTGTAAGILERLGWEPMETLVYHGHAVVLYGCTL"
     gene            3028..4329
                     /gene="aceA"
                     /locus_tag="VEL74_15635"
     CDS             3028..4329
                     /gene="aceA"
                     /locus_tag="VEL74_15635"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HYG64007.1"
                     /translation="MSENARAEILETEQSWKDDSRWKGIQRPYTAADVDRLRGSVRIE
                     HTLARMGAERLWELLHSEPYVAALGALTGNQAIQQVQAGLQAIYLSGWQVAGDANEAG
                     EMYPDQSLYPVDSVPKVVRRINSALQRADQIQHAEGGDGPYWFAPIVADAEAGFGGNL
                     NAFELMKDMIEAGAAGVHFEDQLSSAKKCGHLGGKVLVPSSEFEQKLIAARLAADVCG
                     VPTLVVARTDANSAKLITSDVDPRDQPFIEKERTVEGFFKLRGGLDAAIARGLIYAPY
                     SDMIWCETSRPDLDEARQFAEAIHDKFPGKLLAYNCSPSFNWKHHLDAPTIAKFQREL
                     GAMEYKFQFVTLAGFHALNLSMFELARAYKETGMTAYSRLQEREFENEKLAGYRAVKH
                     QRFVGTGYFDQVAQVISSGHSSTVALEGSTEQEQFEEKVAS"
     gene            4331..5908
                     /gene="aceB"
                     /locus_tag="VEL74_15640"
     CDS             4331..5908
                     /gene="aceB"
                     /locus_tag="VEL74_15640"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HYG64008.1"
                     /translation="MSAHTAEPVQVAEPAADSRDAHARVLTPEALDFVADLQRRLGPG
                     REELLAHRESRLEDFRRGDLPGFPDATATARSGDWRVAPAPRDLEDRRVEITGPVERK
                     MMINALNSGASVFMADFEDSLSPTWANVVQGQANLMDAVRREIEFESPEGKRYTLKER
                     TATLLVRPRGWHLPERHVTVDGRPVSASLFDFGLYFFHNAAELLRRGTGPYFYLPKLE
                     SAAEARLWNDAFAAAQEALGIPRGTIRATVLIETITAAFEMEEILYELREHAAGLNAG
                     RWDYIFSTIRKLPGSLLPDRAQVTMGVPFMRAYAELLVSTCHRRGAHAIGGMAAFIPS
                     RRDAEVNRVALEKVREDKEREARAGFDGTWVAHPDLLPVAREIFDRHLGDRPHQKHVL
                     RDDVKVKAEQLLDFNIEGGRVSEEGIRTNIAVTLLYLDSWLQGSGAVGINNLMEDTAT
                     AEISRAQLWQWLRHQVELPDGRTFTPELYRQIREEESAKLGEQGRLREAAGILDSLVL
                     SRDFIPFLTVAAYERLD"
     gene            5992..6240
                     /locus_tag="VEL74_15645"
     CDS             5992..6240
                     /locus_tag="VEL74_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64009.1"
                     /translation="MKVRALGFFAALSLSVLAAQAQSGWTLEGCWSPYPNCVGAKDVY
                     RDSTGQLWQCRACGTTTNPSPTTCYKSSGLYSIGYWCS"
     gene            6299..6601
                     /locus_tag="VEL74_15650"
     CDS             6299..6601
                     /locus_tag="VEL74_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64010.1"
                     /translation="MMRARSLVLPLVCLLALALVLPLAGLEGECDDCLDGGEPGCCPP
                     SCSLCVCCPHGQSVLAGGTGMERAPAVSSHSGGAIENALPSADPRDVFHVPKPFLV"
     gene            6678..7901
                     /locus_tag="VEL74_15655"
     CDS             6678..7901
                     /locus_tag="VEL74_15655"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYG64011.1"
                     /translation="MRTRFVWPLPAIVGLALWALPALGQERMLTLEGALDLARERGAG
                     VALAQGRVEEARARQGQAARRFQENPALEVNGGYRRAEEDHLDFEAALSQDLYTGRTR
                     EARLAGTRAAFERAEAELEDARRLLLREVWTTFARAAVAGDRVTLVARSRQAADELLA
                     ASERRYEAGEATALELNRARTAAATARAEQSGAEAERDAALAELKALLGLAAGEAVTV
                     RKMELRPPLELDVLLAGLDRRPDLRALAAELREAEAEVLLGRSLARPGLGVRGGLARE
                     EGAEIITAGVVVTLPVHNRGQETVAVGEARAAALRQALETARAAAAAEVRGQHSALSR
                     RLAAVRELEQTALPALDDNESLALKSFDAGEIDLGELLLIRREILETRLAHLERLLEA
                     ALTRFELEAAAGALP"
     gene            7898..9112
                     /locus_tag="VEL74_15660"
     CDS             7898..9112
                     /locus_tag="VEL74_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYG64012.1"
                     /translation="MNAKTWMVWIALLLPLFGCGAAPKEEEHAHEAGEHAEEEPAGEE
                     HEGEHGGEAADEEGVLVVDTEVLRDLKLTTFPAELRPGGEGVTALGELKVNEVAYAEA
                     GSPIPARVVRLVAAPGQFVRRGQPLAELQSVELGQARAARTAALARVELARQTVERKR
                     GLAAERIVSRGELQRAEAAAAEAEAELRAADAALEALGVGKSGSGGLSGFSLVSPISG
                     TVLERTAVQGQTADPARALFRIGDLSRLWLVVQAPERDAVRVRTGAPAEIALAALPGQ
                     KLRGTVEWIGREVDAHSRTVPVRLVIPNEGGNLKPGMFATAWIGTGGEGERVVAVPAT
                     ALQRMDDRWIVFLPRGEGRFEVRPVERGRDLGDEVAVLSGLRPGERVVVEGAFVLRAE
                     AEKGEGGHEHHH"
     gene            9122..12220
                     /locus_tag="VEL74_15665"
     CDS             9122..12220
                     /locus_tag="VEL74_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYG64013.1"
                     /translation="MIERILRGSFARPALTLLLALAAAVAGAVWLTDLRRDVFPDLAT
                     PVFSVYVQNAAMGAEELETGVAIPLEVALAGLPEVRRIRSSSLAGVALVRVEFEPDAG
                     YYLSRQLVTERVNQVARELPPGTGAPLVSSLTGRLNEVMELVIEAEPGAAGLMELRDL
                     ADYEVRNRLLGVPGVAAVEVLGGHLRQFQVQLDPERMAALGVTLDEVMTAVRRSNENA
                     AGGFVAQGPVEWTVRAVGRAENVGDLSTTVVAVRDTTPVLLNEVADVREAPAVRRGMA
                     HGLAEEIVSFRVTKQFGADTVAVARGVRAAVEGLAASMPEGVRVRVLYDQAELVSSAL
                     GGVGRAVLLGALLVVAVLFLLLGNLRAALIVTLTLPLSIALAGLMLKPAGIGLNTLTL
                     GGLAIAVGLLVDAAIILTENVYHRLTAAARSSPAGDTAARREVVLAAALEVGRPISFA
                     TLIVIAVFSPLFAMSGIEGRMYRPLAAAVVAAMAASLVLALTLVPVLAGWLLRPAPPG
                     KEEDVALIRRVKALYAPLLERCLRRAGLVRVLALAVTIPALLLAFRIGSDFMPRLDEG
                     ALLLQTILPPEASLEEVDRRNHRVEDLLRRFPEVKEVVRRTGRAERTEDPMPHVISDV
                     LVILQPGRSRPLEELEAEMRERLEKVPGVAVLFTTPLGMRIDEGLGGTPADIQVRIYG
                     PDLDVLARLAGEARELMEPIAGITDLRAETLTGLPQVRITVDREAVARVGLAPADVVD
                     ALRIGLAGEEVSQVWVGQRRFDLVVRLADDRRGDLSALSSLLIDGRDGTRVPLGQLAR
                     VERTFGPAAIQREAGSRRIAVEAGAAGRDLGSTAAEVRERLAEELELPTGYFFDIGGR
                     VENQARALRSLGLAIAVALLAVLVLLYLALGSFAETMVILATLPDAFVGGILALWLAG
                     ETWNVSSLVGLIGLFGIAVQNGLVLVSQTRSLLAEGRPFEEALREASIGRVRPKLMTA
                     GTAILGLLPLLVLNLHGSEIERPLAIVMIGGLVTSTLFTLLALPTFYQWVHQMQGRFG
                     RR"
     gene            complement(12268..12804)
                     /locus_tag="VEL74_15670"
     CDS             complement(12268..12804)
                     /locus_tag="VEL74_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64014.1"
                     /translation="MCSEKDGTNVPTPSSPASSSTLPNAFDAAFLARLQDRDEPPAAE
                     EADWAGPWRLDRLPDGGLGFFRAGESTERGHRPVARFRGESDALLTMAIIASRGEAAY
                     RLRALPDGRGYAVQSRAAWGEDVGWLAVFDQDLVNALSWAEGLMRSPEALSFFLEACG
                     RTVLERAGAILEERVGAE"
     gene            complement(13073..13324)
                     /locus_tag="VEL74_15675"
     CDS             complement(13073..13324)
                     /locus_tag="VEL74_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64015.1"
                     /translation="MKADRGMASASLEADVIPREREGVSEEMTSISSEMATISEGVKG
                     ISRERRTASREMVDVSGEMLATSSEMVAASGQTVVGRVC"
     gene            13488..13877
                     /locus_tag="VEL74_15680"
     CDS             13488..13877
                     /locus_tag="VEL74_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64016.1"
                     /translation="MPAESFGESVSAMEQLVANAEPRVAGQPLLEGVLTELKDMLVEV
                     RDLKKHQRETRATAQAATKQMLASLTRAQDLAARLRTGLTTLFGKYDERLVEFGIRPI
                     RRRPQKGPIVSPVEDADPAETTGTTTS"
     gene            14060..14230
                     /locus_tag="VEL74_15685"
     CDS             14060..14230
                     /locus_tag="VEL74_15685"
                     /inference="COORDINATES: protein motif:HMM:NF038153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I lanthipeptide"
                     /protein_id="HYG64017.1"
                     /translation="MKKSKKKLVLAKETVRSLETSALRDVAVIGASGEYACNPLTGMS
                     CRYCLDEPIGPA"
     gene            complement(14281..15357)
                     /locus_tag="VEL74_15690"
     CDS             complement(14281..15357)
                     /locus_tag="VEL74_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HYG64018.1"
                     /translation="MSGTDRLDIAHRRLRSQRIAGTGFKKPGEVVAWLGAVQAQDYLG
                     SLWALGTRMKTATEEAVEQAIADRTVVRTWPMRGTLHFVAPADVRWMLALLTPRVIAG
                     TQWRYRQLDLDQALFDRSRDLLTRTLEGGRQITRGALFEILDAAGIATTGQRGINILN
                     RLAMDGLLCFAARQGKQPAFALLEEWVPPGRMLERDEALAELAGRYFTSHGPATLQDF
                     TWWSGLTVADTKLGIELAGSRLEKEVFEGRDYWSVAAETGTGPAPRAYLLPPFDEYTV
                     AYKDRSAVLDPAHGGVMSSNGIFYPTIVLDGQVIGTWKRTFKKGSVVIALSPYAPIKK
                     KDRKAVAEAAERYGAFLGMPAVVE"
     gene            15422..>15989
                     /locus_tag="VEL74_15695"
     CDS             15422..>15989
                     /locus_tag="VEL74_15695"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYG64019.1"
                     /translation="METLGISPPPLTPLAPGTTPEATAAILGAIQELNASADLRSGLA
                     RVAEHLRSCIAYDSLGILLLDDLGRELKFEFTAGIPPEVAEHWRFGLGQGIVGTVARE
                     RRPLRVGDVARDARYIRASDGMKSELAVPLLVRERAIGVLDVCSARAHFFTEEHERLL
                     AFLAGFLAGAIENARLYKNLHEQARTLSV"
BASE COUNT         2374 a         5694 c         5528 g         2393 t
ORIGIN      
        1 cgatctgctg gaggaggttc tccatctcct cggcgtagag gacgcgcttg gagtcgctgg
       61 ggatccagaa ctggaacggc tgctcggggc tgggccggaa gacgacggcc ttgcgctcgc
      121 gcggcttgcc ctcctcgtcg atccccccct tccaggtcca gatctcgagc ggccggaagg
      181 cgttgccgca gtcgatgggc ttgcgctcgg ccggggggcc gttgaggaac aggagctgcg
      241 cccgcacgtc cgtaggggac atgtattcga tggcggccag ccgccgccgg cgctcgatcg
      301 cctccgtcag ctcgttgccc cgggtctcgg ggatcgggtc ctgggacagg aaggtccgga
      361 tgaactgctc ccgcccgtcg gcgtccaggg agaggagctc gtcgcgctgg gcctcggtga
      421 gcaggagcgc cgggccgtcc tgccagaacg accgctgcgc ctcgggccaa agggtgacgt
      481 cgacctggcg ctccgccggc cgggtcacct gccgccgcgc catctcggac acgctggtcc
      541 gttcctcctg gcggggtgga tcgaactggt cgggctggcg cgcggtccgg ggcggcccgg
      601 gtgggcgcga acgagtcgcc tgggacagcg cgcgctccgg cgtgacgaag gaggagacga
      661 gggcggacag cagcagggcg agcgggaata tagcctttgt gcgcatggct ttagggggaa
      721 cccattgcat ggccggtgcc attcccctgg tcctttcttg tcacctgtcg ggagaggagc
      781 cgccattggc agcggcgcgg gaagagacta gtatggctcc gagtccctat gtccaagagc
      841 cgccgcaacg ccccgatcca tagctgggaa gccggagaca acgtccaggg cttcgcgctc
      901 ctcaccaaga aggagatgcg ccaggaccgt aacggtaaga gcttcctgga cttggagctc
      961 accgacgcca ccggtgccat cgtcgccaag gtgtgggccg acagcccggc cctgcacggc
     1021 cagtacgaga cgcactcctt catcgccttc cgcgggtcgg tcaagagcta ccgtgaccag
     1081 ctccagctca cgatcgacga ttgccgctgc gccacggagg acgaccgccg cctcggtttc
     1141 gacgaagcgc tgctcattcc gtccactcac gaggacatcg atgacctctg ggcgcggctg
     1201 gtccgcatct gcgaggagga ggtggaccgg ccggtgctgc ggcggctggc ggcggagacg
     1261 ctggagctct acaagaagga gctgcgggag cacccggcgg cgaagtcgat gcaccacgcc
     1321 taccgcggcg gcctgctgga gcacgtggtg tcgatggcgg agctggccgt ccaggtctgc
     1381 cgccactatc aggacctgga ccgcgacctg atcctcctcg gggtgctctt ccacgacctg
     1441 ggcaagctcc aggagctggg ggcgatgccg gccaacgact acaccctgga ggggcgcctg
     1501 atcggccaca tcgtcctcgg gcgggacctg ctgcgcgagc gctgcgcggc aatcccggac
     1561 ttcccggcgg acctgcggct gattcttgag cacctggtgc tgtcccacca ggggaagaag
     1621 gagttcagct cgccggtcga gccgatgacg ccggaggcgc tggcgctgca cttcatcgac
     1681 gacctggact cgaagctgaa ccagctccgc cgcgcccgcg agctgtcgcc cggcatgcag
     1741 ttccaccggg ggctgggccg cttcgtctac ctccccgcgc cggtcgagcc cagcctcgac
     1801 gacaccatgg tcgccctccc cggccgtccc gaggagatgg aggtgggacg gaccgccccg
     1861 gtgcccgggg ccgtggtgat cgaggtcacg gagagcggcg caccggccca gcctgaggaa
     1921 gcgcctcagc cgcggctgga cttcgggagg taaggcatcc tttagccaac gtcttggcat
     1981 tcgcagcagc ctgcgctgca taccatctgg agcaccaacc cagtgaagac ctcctcgcag
     2041 tacccgtagc agtccgcgcc attccaccga gggttgcagg tcggtacacc aggatcacat
     2101 tgcgcactct tatcgctgga gctgttctgg ttcttactgc aatgtcctga ggtgaggaag
     2161 ggaggagcag ggggagaggc cgcaggagtc tgtagaagct gaacggcaga cggcgtggca
     2221 tcggctccgg atgccaggac cgagctggct gccaaacaga agatcccgac gagaaaggtg
     2281 gccaagatac tcaggttgcg catgggattt gtctcctcct aggtttatag gtccaaggcc
     2341 tccgccgtca acatcattga ataatagcct aaattaaaac gagggcttga gtgcatttac
     2401 ggatcgccta tctctccgat caccccgaag ccatctccac cctcgcccgc tggttccagg
     2461 aggagtctcc ggactacttc gcgggctggg ctcccgagga gatggagcag catttccagc
     2521 cctggctgca ccgcgaccgc ctgccgctcg ggctcgtcgc cttcgagcgg ggcgaggtcg
     2581 tcggctgcat cgtgctgcgc gagaaggcgc tcgaccgcga accggcgcac agccccgggc
     2641 tgggcgggct gcacgtggcc gagccgcacc gggggcgtgg catcggatcg gcgctggtca
     2701 aggcgggcat ggacgcggcg cgggcgctcg gctacgacgc ggtctacacc ggaacgggga
     2761 ccgcggccgg gatcctggag cggctcgggt gggagccgat ggagacgctc gtctaccacg
     2821 gacacgcggt cgttctctac ggctgcacac tgtaagccgc ggcgcggcta gcctctggcg
     2881 cgattcctgc aatcgagcac cagacgtgca gctttgatgc tttcctgtgt cttctctctt
     2941 ctgtaggccg cgataaggct ctcgacgcaa tgggcgtcga ggctttagcc atggacaact
     3001 tgcaggccac cggaggagag agacttcatg tccgagaatg cgcgcgccga gatccttgag
     3061 accgaacaga gctggaagga cgattcgcgc tggaagggga tccagcggcc gtacaccgcc
     3121 gccgacgtcg accgtctgcg cggctcggtg aggatcgagc acacgctcgc ccgcatgggg
     3181 gccgagcggc tgtgggagct gctccattcc gagccctatg tggcggccct cggggcgctc
     3241 accggcaacc aggcgatcca gcaggtgcag gcggggctcc aggcgatcta cctcagcggt
     3301 tggcaggtgg ctggcgacgc caacgaggcc ggcgagatgt acccggacca gagcctctat
     3361 ccggtggaca gcgtgccgaa ggtggtgcgc cgcatcaact ccgccctgca gcgcgccgac
     3421 cagatccagc acgccgaggg cggcgacggg ccctactggt tcgcccccat cgtcgccgac
     3481 gccgaggccg gcttcggcgg caacctcaac gccttcgagc tgatgaagga catgatcgag
     3541 gcgggcgccg ccggcgtcca cttcgaagac cagctctcct cggccaagaa gtgcggccac
     3601 ctgggcggca aggtcctggt gccgtcgtcc gagttcgagc agaagctgat cgccgcccgc
     3661 ctcgccgccg acgtctgcgg cgtccccacc ctggtcgtcg cccgcaccga cgccaacagc
     3721 gccaagctca tcaccagcga cgtcgacccg cgcgaccagc cgttcatcga gaaggaacgg
     3781 acggtcgagg gcttcttcaa gctgcgcggc ggcctcgacg ccgccatcgc ccgcggcctg
     3841 atctatgccc cctactccga catgatctgg tgcgaaacct cgcgcccgga cctcgacgag
     3901 gcccgccagt tcgccgaagc gatccacgac aagttcccgg gcaagctcct ggcctacaac
     3961 tgctccccct ccttcaactg gaagcaccac ctcgacgcgc cgaccatcgc caagttccag
     4021 cgcgagctcg gcgccatgga gtacaagttc cagttcgtca cgctggccgg cttccacgcc
     4081 ctcaacctct cgatgttcga gctggcgcgc gcctacaagg agaccggcat gaccgcctac
     4141 tcgcggctcc aggagcgcga gttcgagaac gagaagctcg ccggctaccg cgcggtcaag
     4201 caccaacgct tcgtcggcac cggctacttc gaccaggtcg cccaggtgat ctcgagcggc
     4261 cactcctcca ccgtcgccct cgaaggctcc accgagcagg agcagttcga ggagaaggtg
     4321 gcgagctgag atgagcgccc atacagccga gccggtccag gtggccgagc ccgcggcgga
     4381 ctccagggac gcccacgcga gagtgctgac gccggaggcc ctggacttcg tggcggatct
     4441 ccagcgccgc ctcggtccgg gccgggagga gctgctggcc caccgggagt cccgtctgga
     4501 ggatttccgg cggggggacc tccccggctt cccggatgcc accgccaccg cccgctccgg
     4561 cgactggcgc gtcgcccccg ctccccgcga cctcgaggac cgccgggtcg agatcaccgg
     4621 cccggtggaa cgcaagatga tgatcaacgc cctcaactcg ggggcgagcg tcttcatggc
     4681 cgacttcgag gactccctct cccccacctg ggccaacgtt gtccaggggc aggcgaacct
     4741 gatggacgcg gtccgccgcg agatcgaatt cgagagcccc gagggcaagc gctacacctt
     4801 gaaggagcgg acggccaccc tcctggtgcg cccgcgcggc tggcacctgc cggagcggca
     4861 cgtgacggtg gacggccggc cggtctcggc gagcctgttc gacttcggcc tctacttctt
     4921 ccacaacgcc gcggagctgc tgcgccgcgg caccggcccc tacttctatc tgcccaagct
     4981 ggagagcgcc gccgaggcgc ggctctggaa cgacgccttc gccgccgccc aggaggcgct
     5041 gggcatcccg cgcggcacca tccgcgccac cgtgctcatc gagaccatca ccgccgcctt
     5101 cgagatggag gagatcctct acgagctgcg ggagcacgcc gcgggcctga acgctggccg
     5161 ctgggactac atcttcagca ccatccgcaa gctgcccggg tcgctgctgc ccgaccgcgc
     5221 ccaggtcacc atgggagtgc cgttcatgcg cgcctacgcg gagctgctgg tgagcacctg
     5281 tcaccggcgc ggcgcccacg ccatcggcgg catggcggcg ttcatcccca gccggcgtga
     5341 cgccgaggtc aaccgcgtgg ctctcgagaa ggtgcgggag gacaaggagc gggaggcccg
     5401 cgccggcttc gacggcacct gggtcgccca ccccgacctc cttcccgtgg cccgcgagat
     5461 cttcgaccgc cacctgggcg accggccgca ccagaagcac gtcctgcgcg acgacgtgaa
     5521 ggtgaaggcg gagcaactcc tcgatttcaa catcgagggg ggccgggtga gcgaggaggg
     5581 gatccgcacc aacatcgccg tcacccttct ctatctcgac tcctggctcc aggggtcggg
     5641 cgccgtgggc atcaacaacc tgatggagga cacggccacg gcggagatct cccgcgccca
     5701 gctctggcag tggctccgtc accaggtgga gctgccggac ggccgcacct tcacccccga
     5761 gctgtaccgg cagatccgcg aggaggagtc ggcgaagctc ggcgagcagg gacggctacg
     5821 cgaggccgcc gggatcctcg actcgctggt cctctcgcgc gacttcatcc cgttcctgac
     5881 cgtggcggcc tacgagcggc tggattgagc gcggagacgg gttcgacagg caccttgata
     5941 gagggtggtc ttcgggcagt caggcccgac accctcgtaa aggagactgc catgaaagtt
     6001 cgagctctag gtttcttcgc cgcactctcc ctcagcgtcc tcgcggccca ggcccagtcc
     6061 ggctggaccc tcgaagggtg ctggtcgccc tacccgaact gcgtcggcgc caaggacgtc
     6121 tatcgcgatt ccaccggcca gctctggcag tgccgcgcct gcgggacgac caccaatccc
     6181 agccccacga cctgctacaa gagcagcggc ctctacagca tcggctactg gtgctcctag
     6241 tctctctggg gacgcctccc atctcgggag gcgtccccga gctgtgatat acgtcctgat
     6301 gatgcgtgcg cgttcgctgg ttctcccgct cgtctgcctc ctggccctgg cgctcgtgct
     6361 gcctctggcc ggccttgagg gcgagtgcga cgactgcctg gacggtggcg agcccggctg
     6421 ctgcccgccg tcctgctccc tctgcgtgtg ctgcccgcac ggccagtcgg tcctggccgg
     6481 aggcaccgga atggagcgtg ctccggcggt ctcctcccac tccggcggcg cgatcgagaa
     6541 tgcgctcccc tccgcggacc cccgcgacgt cttccacgtc cccaaaccct tcctcgtcta
     6601 gctcacccgt cgccgttcag ccacgccgcg cacccggact agggtgtgtg ggcggatctg
     6661 ccttcgtgag gtgaccgatg aggactcgat tcgtctggcc gctgcctgca atagttgggc
     6721 tggccctctg ggccctgccg gcgctcggcc aggagaggat gttgacgctc gaaggggccc
     6781 tggacctggc gcgggagcgc ggcgccggcg tggccctcgc ccagggccgg gtggaggagg
     6841 cccgggcgcg gcagggccag gcggcgaggc ggttccagga gaacccggcg ctggaggtga
     6901 acggcggcta ccggcgcgcc gaggaggacc acctcgattt cgaggcggcc ctctcccagg
     6961 acctctacac cgggcgcacg cgggaggccc gcctcgccgg cacccgggcg gccttcgagc
     7021 gcgccgaggc ggagctggag gacgcgcggc ggctgctcct gcgcgaggtc tggaccacgt
     7081 tcgcccgcgc cgccgtcgcc ggggaccgcg tcacgctggt ggcacggagc cgccaggcgg
     7141 ccgacgagtt gctcgccgcc agcgagcggc gctacgaggc gggcgaggcc acggccctgg
     7201 agctcaaccg cgcccgcacc gccgcggcca cggcccgggc cgagcagagc ggcgccgagg
     7261 ccgagaggga cgccgccctc gccgagctga aggcgctgct cggcctcgcc gccggagaag
     7321 cggtcacggt ccggaaaatg gagctccgtc cgccactgga gctggacgtg ctcctcgccg
     7381 ggctcgaccg ccggccggac ctgcgggccc tcgccgccga gttgcgcgag gcggaggccg
     7441 aagtccttct cggccggtcc ctggcccggc ccgggttggg agtgcgcggc ggcctcgccc
     7501 gcgaggaggg ggcggagatc atcaccgccg gcgtggtcgt caccctgccg gtccacaacc
     7561 ggggccagga gacggtcgcg gtgggcgagg cccgggccgc cgccctccgc caagccctgg
     7621 agaccgcccg cgccgccgcc gctgcggagg tccggggcca acactccgcc ctgtcgcggc
     7681 ggctcgcggc ggtacgggag ctggagcaga ccgccctccc cgccctcgac gacaacgaat
     7741 ccctcgcgct caagagcttc gacgccggcg agatcgacct gggggagctc ctcctgatcc
     7801 gccgcgagat cctggaaacc cgcctcgccc acctggaacg cctcctcgaa gccgcattga
     7861 cccgcttcga gctggaagcc gccgccggag ccctgccatg aacgcgaaga cctggatggt
     7921 ctggatcgcc ctcctcctgc ctctcttcgg atgtggcgcc gccccgaagg aggaggagca
     7981 tgcccatgag gccggggagc acgccgaaga agagcctgcc ggcgaggagc acgagggcga
     8041 gcacggcggc gaggccgcag acgaagaggg ggtgctcgtc gtggacaccg aggtgctccg
     8101 ggacctcaag ctcaccacct tcccggccga gctgcggccg ggcggcgagg gggtgaccgc
     8161 cctcggcgag ctgaaggtga acgaggtcgc ctatgccgag gccggctctc ccatccccgc
     8221 gcgggtggtc cgcctcgtcg cggccccagg ccagttcgtc cggcgtggcc agcccctcgc
     8281 ggagctgcag agcgtcgagc tcggccaggc gcgggcggcg cggaccgctg ccctcgcccg
     8341 cgtcgagctg gcgcgccaga cggtcgagag gaagcgcggc cttgccgccg agcgcatcgt
     8401 ctcccgcggc gagctgcaac gcgccgaggc cgccgccgcc gaggccgaag cggagctgcg
     8461 ggccgccgac gccgctctcg aagccttggg ggtgggaaag agtggcagcg gcgggctctc
     8521 cggcttctcc ctggtctccc ccatctccgg caccgtgctg gagcgcaccg ccgtccaggg
     8581 gcagaccgcc gaccccgcgc gcgccctgtt ccgcatcggc gacctgtcgc gcctctggct
     8641 ggtggtccag gcgccggagc gcgacgccgt ccgggtgcgc acgggcgcgc cggccgagat
     8701 cgcgctggcg gccctcccgg gccagaagct gcgcggcacc gtcgagtgga tcggccgcga
     8761 ggtcgatgcc cattcgcgca ccgtccccgt ccgcctggtc atccccaacg agggagggaa
     8821 cctcaagccg gggatgttcg ccaccgcctg gatcggcacc ggtggggagg gagaaagagt
     8881 ggtggcggtg ccggcgacgg ctctccagag gatggacgac cgctggatcg tcttcctgcc
     8941 gcgcggcgag gggcggttcg aggtacggcc ggtggagcgc gggcgcgacc tcggcgacga
     9001 ggtggcggtc ctctccggcc tgcggccggg ggagcgcgtc gtggtcgagg gggccttcgt
     9061 gctgcgcgcg gaggccgaga agggcgaggg tggccatgag caccaccact aggagcaccc
     9121 gatgatcgaa cgaatcctgc gcggctcctt cgcccggccc gccctgaccc tcctcctggc
     9181 cctcgccgcg gcggtggccg gcgcggtctg gctcaccgat ctgcggcgcg acgtcttccc
     9241 cgacctggcg acgccggtgt tcagcgtcta cgtccagaac gcggcgatgg gcgccgagga
     9301 gctggagacc ggcgtcgcga tccccctgga ggtcgcgctc gccggcctgc ccgaggtgcg
     9361 ccgcatccgc tccagctcgc tggccggcgt cgccctggtg cgcgtcgagt tcgagcccga
     9421 cgccggctat tacctctccc gtcagctcgt caccgagcgg gtcaaccagg tcgcccggga
     9481 gctccccccg gggaccggcg cgccgctggt ctccagcctc acgggccggc tcaacgaggt
     9541 catggagctc gtcatcgagg ccgagccggg cgccgccggc ctcatggagc tgcgcgacct
     9601 cgcggactac gaggtccgca accgcctcct cggcgtgccc ggcgtcgcgg ccgtggaggt
     9661 gctggggggc cacctccggc agttccaggt gcagctcgac cccgagcgca tggccgccct
     9721 cggcgtcacc ctcgacgagg tgatgaccgc cgtccggcgc tccaacgaga acgcggccgg
     9781 cggcttcgtc gcccaggggc cggtcgagtg gacggtgcgc gcggtcggcc gggcggagaa
     9841 cgtcggcgac ctgagcacga ccgtggtggc ggtgcgcgac accacccccg tgctgctgaa
     9901 cgaggtggcc gacgtgcggg aggcgccggc ggtgcgccgc ggcatggcgc acggcttggc
     9961 cgaggagatc gtcagcttcc gggtcaccaa gcagttcggc gccgacaccg tcgccgtggc
    10021 ccggggcgtc cgcgccgccg tcgaggggct cgcggcgagc atgccggagg gggtgcgggt
    10081 gcgcgtcctc tacgaccagg cggagctggt ctcgtccgcc ctcggcggcg tgggccgggc
    10141 ggtgctcctc ggcgcgctgc tggtggtggc cgtcctcttc ctcctcctgg gcaacctgcg
    10201 ggcggcgctc atcgtcaccc tcaccctgcc gctctccatc gccctcgccg gcctgatgtt
    10261 gaagcccgcc ggcatcggcc tcaacaccct gaccctcggc ggcctcgcca tcgccgtggg
    10321 cctgctggtg gacgcggcca tcatcctcac cgagaacgtc taccaccggc tgaccgccgc
    10381 ggcgcggtcg tcgccggcgg gggataccgc ggctcgccgt gaggtggtgc tcgccgccgc
    10441 cctggaggtc ggccggccca tctccttcgc caccctgata gtgatcgcgg tgttcagccc
    10501 gctgttcgcg atgagcggca tcgaggggcg catgtaccgg ccgctggccg ccgctgtggt
    10561 cgccgccatg gccgcctccc tcgtgctcgc cctgaccctg gtgccggtcc tcgccggctg
    10621 gctgctccgg cccgcgcctc ccgggaagga ggaagacgtg gcgctgatcc gccgcgtcaa
    10681 ggcgctgtac gcgccgctcc tcgagcgctg ccttcgccgc gcgggcctgg tacgggtgct
    10741 ggccctcgcc gtcaccatcc cggccctgct cctcgccttc cggatcggct ccgacttcat
    10801 gccgcgactg gacgaggggg cgctgctcct ccagaccatc ctgccgccgg aggcctccct
    10861 ggaagaggtg gaccgccgca accaccgggt ggaggacctc ctgcgccgct tccccgaggt
    10921 gaaggaggtc gtgcgccgca ccggccgcgc cgagcgcacc gaggacccta tgccgcacgt
    10981 gatctcggac gtgctggtga tcctccagcc cggccgctcc cgtccgctgg aggagctgga
    11041 ggcggagatg cgggagcggc tggagaaggt gccgggcgtg gccgtgctct tcaccacccc
    11101 gctgggcatg cggatcgacg aggggctggg gggcacgccg gccgacatcc aggtgcggat
    11161 ctacggcccc gacctcgacg tcctggcgcg cctggccggg gaggcgcggg agctgatgga
    11221 gcccatcgcg gggatcaccg acctgcgggc cgagaccctg accggcctgc cgcaggtgcg
    11281 catcaccgtc gaccgcgagg cggtggcgcg ggtggggctg gcgccggcgg acgtggtcga
    11341 cgccctgcgc atcggcctgg cgggcgagga ggtgtcgcag gtctgggtgg gccagcgccg
    11401 cttcgacctc gtggtgcggc tggcggacga ccggcgcggc gacctctcgg ccctctcctc
    11461 gctcctcatc gacggccgcg acggcacccg cgtccccctg ggccagctcg cccgggtgga
    11521 gcggaccttc ggccccgccg ccatccagcg cgaggccggc agccgccgga tcgcggtcga
    11581 ggcgggcgcc gccggccggg acctggggag cacggccgcc gaggtgcgtg agaggctcgc
    11641 cgaggagctg gagctgccca ccggatactt cttcgacatc ggcggccggg tcgagaacca
    11701 ggcgcgggcg ctgcgctcgc tgggcctcgc catcgcggtg gccctgctgg cggtgcttgt
    11761 cctcctctat ctcgccctgg gctccttcgc cgagacgatg gtgatcctcg ccaccctgcc
    11821 ggacgccttc gtcggtggca tcctcgccct ctggctcgcc ggcgagacct ggaacgtctc
    11881 ctcgctggta ggcctcatcg gcctcttcgg catcgcggtc cagaacggcc tcgtcctggt
    11941 ctcgcagacc cgctcgctgc tggcggaggg gcgtcccttc gaggaggcgc tgcgggaggc
    12001 gtcgatcggc agggtgaggc cgaagctgat gacggcgggc acggcgatcc tgggcctgct
    12061 gccgctgctc gtcctgaacc tccacggctc cgagatcgag cgccccctgg ccatcgtgat
    12121 gatcggcggg ctcgtcacct cgaccctctt caccctcctg gcgctcccca ccttctatca
    12181 gtgggtgcac cagatgcagg ggaggtttgg gaggcgatag gagggcggcc cccgctcgcg
    12241 gtgaccgccc tcctcttctt tactgcgcta ctccgccccc acccgctcct ccaggatcgc
    12301 cccggcgcgc tccagcaccg tccggccgca ggcctccagg aagaacgaca gcgcctccgg
    12361 cgagcgcatc aggccctccg cccaggagag ggcgttgacg agatcctggt cgaagacggc
    12421 gagccagccg acgtcctcgc cccaggcggc gcggctctgc accgcgtagc cgcggccgtc
    12481 cggcagcgcc cgcaggcggt acgccgcctc gcctcgggag gcgatgatcg ccatggtcag
    12541 gagcgcgtcc gactcccccc ggaaccgggc gaccggccgg tgaccgcgct cggtgctctc
    12601 tcccgcccgg aagaagccga ggccgccatc cggcaggcgg tcgaggcgcc acgggccggc
    12661 ccaatccgcc tcctcggccg cgggcggctc gtcccggtcc tgaagacggg ccaggaacgc
    12721 cgcgtcgaag gcgttcggca gggtggacga cgacgctggc gacgacgggg tggggacatt
    12781 ggtaccatcc ttctcactgc acatcagggt cactcctttt gtgtagaggt ccggggtcga
    12841 gttagcgctc ttccccgggc cgcttctgtt ggtccccggc cgcggcagct acccccatgg
    12901 catccgcccg gccggagttt ccccttcaga ccttcaaaga accgcgtccg ttcaacggct
    12961 tactgcaacg gatagcggca gcatagcgga accctggaga ggaacgcaag aggttccggc
    13021 agaaaagttg gaagatctcg cgggaacgac agccagagag ggaagttctc tctcagcaaa
    13081 cccgcccgac gacggtctgg cccgaagcgg ccaccatttc tgaagaagtg gcgagcattt
    13141 cgccagaaac gtcgaccatt tctcgggaag ccgtcctcct ttcccgcgaa atgcccttca
    13201 ccccttcaga aatggtggcc atttctgaag aaatggaggt catctcctcg gaaacgccct
    13261 ccctttctcg agggatgacg tccgcctcca gggaagcgga ggccattcct cggtccgctt
    13321 tcacgatttc tcatctgccc tgttgacaac gccgaatcca cttcgtacta ttctgccatc
    13381 ggatcgtttc cggtctcgat tgcagattca agacttaccg gcctcaccag cccgccatga
    13441 tggtggacgg ttattcgggt gcaatcccga gaaaggagag aagtatcatg cctgctgagt
    13501 catttggaga gagcgtcagc gccatggagc agctcgtcgc caacgcggag ccccgcgtgg
    13561 cagggcagcc gctgctggag ggagtcctca ccgagctgaa ggacatgctc gtggaagtgc
    13621 gggatctcaa gaagcaccag cgcgagaccc gggccacggc ccaggcggcc accaagcaga
    13681 tgctggcgag cctgacccgc gcgcaggact tggccgcccg gctccgcacc ggcctcacca
    13741 cactcttcgg caagtacgat gagcggctgg tcgagttcgg gatccgaccc attcgccgcc
    13801 ggccgcagaa gggcccgatc gtctcccccg tagaggacgc cgacccggcc gagaccaccg
    13861 gcaccaccac cagctaggca acaccacccg caggggcggg cgcgctacca cgcgcccgcc
    13921 cgatctttct tgaatctgcc gctctttgac aactgaatat aaaggataac aacaacgaga
    13981 gaggcggaga tccgatccct cccccctggg cgtacaatgc cctctcgcgc ctggcggcgc
    14041 gtctctcaag gagcggcaaa tgaaaaagag taagaaaaag ctggtgctgg cgaaggaaac
    14101 ggtgaggagc ctcgaaactt cggcattgag agatgtcgcg gtcatcgggg cttcgggtga
    14161 atacgcctgc aatcctctca ccggtatgtc ctgccgctac tgtctcgacg agccgatcgg
    14221 gccagcctga gtcctcacag gggcggcctc tcggccgccc cgcctccttg ctcgccgcgg
    14281 ctactccacg accgcgggca tgccaagaaa agcgccgtaa cgctcggccg cctcggcgac
    14341 ggctttccga tccttcttct tgatcggagc atacggagac agtgcgatga cgaccgatcc
    14401 ctttttgaac gtgcgcttcc aggtacctat cacctggccg tcgaggacga tggtgggata
    14461 gaaaatgccg ttgctggaca tgaccccgcc gtgggccggg tcgagcacgg cgctgcggtc
    14521 cttgtaggcg acggtgtact cgtcgaatgg cggcagcagg taggcgcgcg gcgccggccc
    14581 tgtgcccgtc tccgccgcga ccgaccaata gtcccggcct tcgaagactt ccttctccag
    14641 acgtgagccg gccagctcga tccccagctt ggtgtcggcg acggtcagcc cggaccacca
    14701 ggtgaagtcc tgcagggtag cgggaccatg gctggtgaag tagcgcccgg ccagctcggc
    14761 gagcgcttcg tcgcgttcca gcatccggcc gggcgggacc cactcctcca gcagggcgaa
    14821 ggccggctgc ttgccctggc gggcggcgaa gcagaggagc ccgtccattg ccagccggtt
    14881 caggatgttg atgccacgct ggccggtagt ggcgatcccc gccgcgtcca ggatctcgaa
    14941 cagggcgccg cgcgtgatct gccggcctcc ctccagggtg cgcgtgagga ggtcgcggct
    15001 gcggtcgaag agggcttggt cgagatcgag ctgccggtag cgccactggg ttcccgcgat
    15061 cacccgaggc gtcagcagcg ccagcatcca gcggacgtcg gccggcgcca cgaagtgcag
    15121 cgtgccccgc atcggccagg tgcggaccac cgtgcggtcg gcgatggcct gctcgaccgc
    15181 ctcctccgtg gccgtcttca tccgggtccc gagcgcccag agagagccga gatagtcctg
    15241 ggcctgaacc gcgccgagcc acgcgacgac ctcgccaggc tttttgaaac cggtccccgc
    15301 gatgcgctgg ctgcggagac gacggtgcgc gatgtccagt ctgtccgttc cgctcatgga
    15361 gagatggtat ccgagcccca ggggcgatcc gggagtagac tagaaaccgg agcccacccc
    15421 catggagacc ctcggaatct cgccgccccc cctcaccccc ctggctcccg gcacgacgcc
    15481 ggaggcgacg gcggcgatcc tcggcgcgat ccaggagctc aacgcctcgg ccgacctgcg
    15541 cagcggcctg gcccgggtcg ccgagcacct gcgctcctgc atcgcctacg attccctcgg
    15601 catcctgctg ctggacgacc tcggccggga gctgaagttc gagttcacgg ccggcatccc
    15661 gccggaggtg gcggagcact ggcggttcgg cctgggacaa gggatcgtcg gcaccgtggc
    15721 ccgggagcgc cggccgttgc gggtgggcga cgtcgcccgt gacgcgcgct acatccgcgc
    15781 cagcgacggg atgaagtcgg agctcgccgt gccgctgctg gtgcgggagc gggcgatcgg
    15841 tgtcctcgac gtctgcagcg cgcgcgccca cttcttcacc gaggagcatg agcgcctgct
    15901 ggccttcctc gccggcttct tggccggcgc catcgagaac gcccggctct acaagaacct
    15961 gcacgagcag gcgcggacgc tgtccgtgc
//
LOCUS       DASPJI010000563        10529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_333727, whole genome shotgun sequence.
ACCESSION   DASPJI010000563 DASPJI010000000
VERSION     DASPJI010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10529)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10529
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333727"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..779)
                     /locus_tag="VEL74_15700"
     CDS             complement(156..779)
                     /locus_tag="VEL74_15700"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYG64020.1"
                     /translation="MPTPWSDASDRDLLMAIREGEERALDELIRRKTKALLQLAQGIL
                     GDNEEARDVVQVTFFKIWENRRKFDGKWSPNTWMYRITSNLAIDHLRSRNSRERIQEP
                     VRLHLLHRADSEAHYDFSNLEQHEVGRIFQRLAAALPERQRVVFVLREIEGLETHEVS
                     QILGIRESAVRSQLYNARQSLRQELLRRYPEYAATLPAALLEPKEDA"
     gene            962..2263
                     /locus_tag="VEL74_15705"
     CDS             962..2263
                     /locus_tag="VEL74_15705"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HYG64021.1"
                     /translation="MRSFVLRGLYQAATGAALLAAGPFLLARRGGHYLSTLPGRLGRP
                     PAEPSTGEDGDRGALWLHAVSVGEVGVAATLARALPADLPLLVTTVTPTGQARARASF
                     AGRAAVAYLPFDLGFAVGRFFRRFEPRALVLVEGDYWPLVLAEARRRGLPVAVVNGRV
                     GDRSFQRMRRLRPLLGPLFDGVDRFGVQTAADRDRLLALGVDAARVTVTGNLKYESPE
                     PPAKPDLEAQIHSLASGRPILLAGSTMEGEEEAVLDAFLQIGAARALLILAPRHPERW
                     DAVDAFLRSRGIAVLRRSRPGESPASPAVLLLDSLGELAGLYRIAAASFIGGTLAPTG
                     GHNPLEPARFGVPVAVGPSMHNFRDMAEQFDRAGAWRRVADAAELAATWRGWLDDPAA
                     AREQGARALRLLEANRGAVARTVEMLAPLLAPVGLRVEPPA"
     gene            2260..3270
                     /gene="lpxK"
                     /locus_tag="VEL74_15710"
     CDS             2260..3270
                     /gene="lpxK"
                     /locus_tag="VEL74_15710"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HYG64022.1"
                     /translation="MKSPSPPVSPWQLLYGGAHRLRRQWYRTHGHRLPRPVVSVGNLH
                     WGGTGKTPLTAALAAHLRDRGLAVCILSRGYASRGRGVRVVSVGEGPLLGPLVAGDEP
                     VLLAGELPGVAVVVGPDRHRAGLHALRRLPRTPQLFLLDDGFSHLALHRDLDLVAFPA
                     ADPFGGGRLPPSGRLREPLASIARAHAVLLTGAASSEDGRLLAEALRRHGFQGPGFAS
                     FTRPAPPASLAPGLPVGARVFLVSAIARPDAFARTARELGYDVAGELRFPDHHRYPAA
                     SLERIESGFREAGAATLLTTAKDAVKLLGRLDLPTAVLPIRAEPEPAFWSWLDGELDR
                     IA"
     gene            3267..4175
                     /locus_tag="VEL74_15715"
     CDS             3267..4175
                     /locus_tag="VEL74_15715"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYG64023.1"
                     /translation="MKNAPFRHRVEYALYTSLKRMLWLLPHPATRTLGRRVGDLGHLL
                     DRRHREVALRNLALALPELSEAERRRLVRECFRHFGSALCDAISSTRFDAVELCRRFH
                     FEGWENLEAAEARGKGSFILSAHLGFWEMVPAVIGLYRGPMHIVVRPADNPHLDRELA
                     ALRERFGHEVVPKRGAARRMVEVLRRAGRVGILIDQRVQRKEGIEVPFFGRPALTSPV
                     LAKLSLRTGAAVIPVTVYPGPEGTWRFVARPAILPPEGVEDTPEAVAALTRRYLEDAE
                     EDIRSHPEMWLWMHRRWEESRAANPK"
     gene            4318..5094
                     /locus_tag="VEL74_15720"
     CDS             4318..5094
                     /locus_tag="VEL74_15720"
                     /inference="COORDINATES: protein motif:HMM:NF012420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HYG64024.1"
                     /translation="MKITRGSFFVFLFALTVTLAGPAVLKAETADACAFGFEGAIGPD
                     HWWEICAPKNLTCAAGQRQSPIDLPAAPAGDLLELRFGYHPTTLEVENTGTTIEVPVE
                     PGTSVLKIGKEEFELLQFHFHTPSEHRLQGEEFPMELHFVHRNALGELAVVGVFLKEG
                     AANPVIQQIWDHAPTAPHTVSEEAGEIDPEDLLPVDRGFYRYAGSLTTPPCSEGVRWH
                     VLQTPVEISAAQVDEFRAIFPVNARPLQLLNGRPVLTDGL"
     gene            complement(5150..5557)
                     /locus_tag="VEL74_15725"
     CDS             complement(5150..5557)
                     /locus_tag="VEL74_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYG64025.1"
                     /translation="MTDHEQANVATARRYLESIERHAPAEEMASFFAPDYVQYEFPNR
                     LMPNGARRNLQDILDAGARGRKVMTSQRYEVINTVAQGDNIVLEVQWTGTLAVPYESI
                     PVGGEMRARFAVFLEFRDGRIVAQRNYDCFDPW"
     gene            complement(5577..6149)
                     /locus_tag="VEL74_15730"
     CDS             complement(5577..6149)
                     /locus_tag="VEL74_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64026.1"
                     /translation="MAASTSLARWISILAHPFVMAVVMAVTTGIRFGALRGGVGAAVA
                     VGLVILPVALLMARQVRAGRWGNVDASEPRERPALFAVAILSLFALFLYLWLRETQAS
                     LLRGVGGTLALVVVAAVANRWIKVSLHMAFACLTVTVLVLLGSIVGWCLVPVIPALAW
                     SRLQLARHRPVELIAGTALGAITGLAVHFL"
     gene            complement(6283..7692)
                     /locus_tag="VEL74_15735"
     CDS             complement(6283..7692)
                     /locus_tag="VEL74_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HYG64027.1"
                     /translation="MPISDFVVSATTTSYAITNWGTSTVIDVASGALVLDAPTGISTQ
                     QWQLIYRGEDQFCLANASTNACIGIDATGAQVSMGAYSGSEAQIWQLVTPGGSANVGP
                     CFLYNNLYGTYLTAQGTGLVLDAAQVVDEFHQWLFNAADPATLQVFNQNSLLSTVTFY
                     LASSAGQYVAVGLQLDEQTYAIVSVQATDSAAVFTIGSDNVITTTAPDGSVLYLGEAF
                     GEPVFTTAASSWSASFMLFNDELTYIRFQDGSYLSLNPSSSQGALVLQPSQFFPGAAF
                     DVVPEAMAPGADLSRFSLPEEVIVTRVDISQLDPCTLAWITLLWQCTGGLLMAVGILP
                     SIANAAMQSTIYNAIVGNATVYSAAQAVVSLIQSNPKMSAALFTLVNELLIQMYNQGL
                     LMPIIKSLISTLGWFALVWLAKKVIIWIFAPQAEVANILVGSIAWGISLVQAGLNVNT
                     SCSSLPEGELPQLVPAGAG"
     gene            complement(7701..8000)
                     /locus_tag="VEL74_15740"
     CDS             complement(7701..8000)
                     /locus_tag="VEL74_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64028.1"
                     /translation="MSIQKFFGDSPDGSVSVDTMNLNDQISFNFTGGSGSGDYWTLQF
                     NLNGVTYYRNRKRCDVETEDLSAPQPVNLNLLPPAQGFSVEMPVSSSCDDNYYDS"
     gene            8295..8453
                     /locus_tag="VEL74_15745"
     CDS             8295..8453
                     /locus_tag="VEL74_15745"
                     /inference="COORDINATES: protein motif:HMM:NF038153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I lanthipeptide"
                     /protein_id="HYG64029.1"
                     /translation="MKKSTKKLVLAKETVRNLEERKLGKVCGAATYTCGYSCGPLQCP
                     PAPYTRDC"
     gene            complement(8545..8871)
                     /locus_tag="VEL74_15750"
     CDS             complement(8545..8871)
                     /locus_tag="VEL74_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64030.1"
                     /translation="MKKKSAPGWPSSDSSRDPQREVVYEPEYLRASEQLSHRILRATE
                     ALQQVESFLSRRPELGLALRSYSPSEFGTWITKPLDGVRIRVLYHYDERTVTMLDAWL
                     IRDSPF"
     gene            9345..9695
                     /locus_tag="VEL74_15755"
     CDS             9345..9695
                     /locus_tag="VEL74_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64031.1"
                     /translation="MPSYSDRTPSRVDLSRPTESTRRDLYELVVHVYFHPTPEEVEKA
                     RQDPGNHWVPDYGPDEARLHVFYAYGRWFAVWEALEEKKETGPLPEWRRWSVVRIVED
                     RDPSSETGVTFLEV"
     gene            complement(10077..10313)
                     /locus_tag="VEL74_15760"
     CDS             complement(10077..10313)
                     /locus_tag="VEL74_15760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HYG64032.1"
                     /translation="MKSLRHHLLEQALQLPLDERAELASEILASLDGEPEPGAEEAWA
                     REIERRAARVLSGESEGIPWEDVRQRIEREVLHR"
     gene            complement(10337..>10529)
                     /gene="yidD"
                     /locus_tag="VEL74_15765"
     CDS             complement(10337..>10529)
                     /gene="yidD"
                     /locus_tag="VEL74_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HYG64033.1"
                     /translation="DLYQATASPMMPKVGVRCRFQPTCSHYSEAAIRKYGTVKGVAKS
                     AWRIARCGPWTPQGTVELP"
BASE COUNT         1692 a         3658 c         3451 g         1728 t
ORIGIN      
        1 ccgccggaac agcagggtcg ggtcggctgc gatggcgcgg gaacggcagt cggaacaggc
       61 gtcgaagtgc ccgagggcct cgcgccaccc ggccggctcc tccccccggc gctcgtgccg
      121 gtgggccgtc aggaccgtcc agtcagggca ggcgttcacg cgtcctcctt cggctcgagc
      181 agggccgccg gcagggtggc cgcgtactcg gggtaccgcc gcagcagctc ctggcgcagc
      241 gactggcggg cattgtagag ctggctgcgc accgccgatt cacggatgcc caggatctgc
      301 gacacctcgt gcgtctccag cccctcgatc tcgcgcagca cgaagaccac ccgctgcctc
      361 tcaggcagcg cggcggccag gcgctggaag atccggccca cctcgtgctg ctccaggttg
      421 gagaagtcgt agtgggcctc ggagtcggcc cggtggagca ggtgcaggcg caccggctcc
      481 tggatgcgct cgcggctgtt gcgcgagcgc aggtggtcga tggcgaggtt gctggtgatg
      541 cggtacatcc aggtgttggg gctccacttg ccgtcgaact tgcggcgatt ctcccagatc
      601 ttgaagaagg tgacctgcac cacgtcccgg gcctcctcgt tgtctccgag gatgccctgg
      661 gcgagctgca ggagcgcctt cgtcttgcgc cggatcagct cgtccagcgc gcgctcctct
      721 ccctccctga tggccatcag gaggtcgcgg tccgaggcat ccgaccaggg agtgggcatc
      781 cgggtgcttc gtgttctgga gtaggcgagc ttgagcatcg tcgatctgcc ctcatctctt
      841 tatacggatg agagcgccaa aacgtccagg tgggtggcca ggaagtcttc cttccccctc
      901 cctcccggcg ctggcagggc gaccggagcc gtgttctcca ggcattatac tgcccgccga
      961 gatgcgctcc ttcgtcctcc ggggcctgta ccaggccgcc accggggccg ccctcctggc
     1021 ggccggcccc tttctcctcg cccgccgggg gggccattat ctctccaccc tgcccggccg
     1081 gctgggccgc ccccccgccg agccgtccac gggcgaggac ggagaccggg gcgccctctg
     1141 gctgcacgcc gtctcggtgg gggaggtggg ggtggcggcg accctggcgc gggcgctgcc
     1201 cgcggacctg ccgctgctcg tcaccaccgt caccccgacc ggccaggccc gcgcccgggc
     1261 cagcttcgcc ggccgcgccg ccgtcgccta cctgccgttc gacctggggt tcgctgtcgg
     1321 gcgcttcttc cggcgcttcg agccgcgggc cctggtgctg gtcgaggggg actactggcc
     1381 cctggtcctc gccgaggcgc ggcggcgcgg cctcccggtc gccgtggtca acggccgggt
     1441 gggggaccgc agcttccagc gcatgcgccg gctgcgcccg ctcctcggac cgctgttcga
     1501 cggcgtcgac cgcttcggcg tccagaccgc ggcggaccgc gaccggctgc tcgcgctggg
     1561 ggtcgacgcc gcccgggtca ccgtcaccgg caacctcaag tacgagtcgc cggagccgcc
     1621 cgccaagccg gacctcgaag cccagatcca ttccctcgcc tccggccggc ccatcctcct
     1681 cgccggctcg accatggagg gggaggagga ggccgtcctc gacgccttcc ttcagatagg
     1741 agccgcccgc gccctcctca tcctcgcccc gcgccacccg gaacgctggg acgcggtcga
     1801 cgcattcctg cgctcccgcg gcatcgccgt cctgcgccgg agccggccgg gggagagccc
     1861 ggcatctccg gccgtcctcc tcctcgacag cctcggggag ctggccggcc tctaccggat
     1921 cgccgccgcc tccttcatcg gcggcaccct cgcccccacc ggcggccaca acccgctgga
     1981 gccggcgcgc ttcggggtgc cggtggcggt cgggccctcc atgcacaact tccgggacat
     2041 ggccgagcag ttcgaccgcg ccggggcctg gcgccgggtg gccgacgccg ccgagctggc
     2101 cgccacctgg cgcggctggc tggacgaccc cgccgccgcc cgcgagcagg gggcccgcgc
     2161 cctgcgcctg ctggaggcga accggggagc cgtcgcccgc accgtggaga tgctcgcccc
     2221 gcttctcgcc ccggtggggc tccgcgtgga gccccccgcg tgaagagccc gtcccccccg
     2281 gtctccccct ggcagctcct ctacggcggc gcccaccgcc tgcgccggca gtggtaccgc
     2341 acccacggac accggctgcc gcggccggtg gtgagcgtcg gcaacctgca ctgggggggc
     2401 accggcaaga cgccgctcac cgccgccctg gccgcccacc ttcgcgaccg cggcctcgcc
     2461 gtctgcatcc tctcgcgcgg ctacgccagc cgcggccggg gggtgcgcgt ggtgagcgtg
     2521 ggagaggggc cgctcctcgg gccgctggtg gccggtgacg agccggtgct cctcgccggc
     2581 gagctccccg gcgtcgcggt ggtggtcggg cccgaccgcc accgcgccgg cctccatgcc
     2641 ctgcgccgcc tcccccgcac cccccagctc ttcctgctcg acgacggctt ctcccacctg
     2701 gcgctgcacc gcgacctgga cctcgtggcc ttccccgccg ccgacccgtt cggcggcggc
     2761 cggctgccgc cgtcggggag gctgcgcgag ccgctcgcct ccatcgcccg cgcccacgcc
     2821 gtgctcctca ccggcgccgc ctcttcagag gatggacgcc tcctcgccga ggcgctccgc
     2881 cgccacggct tccagggccc cggcttcgcc agcttcaccc ggcccgcgcc gccagccagc
     2941 ctggcgcccg gcctgcccgt cggcgccagg gtcttcctgg tctcggccat cgcccggccg
     3001 gacgccttcg cccgcaccgc ccgcgagctc ggctacgacg tcgccggcga gctgcgcttc
     3061 cccgaccacc accgctaccc agccgcgagc ctggagcgga tcgagtccgg cttccgcgag
     3121 gcgggcgccg ccaccctgct caccacggcc aaggatgcgg tcaagctcct cggccgcctc
     3181 gatctgccca cggccgtgct gcccatccgc gccgagccgg agcccgcctt ctggagttgg
     3241 ctggacggag agctggacag gatcgcttga agaacgcccc gttccgccac cgcgtcgagt
     3301 acgccctcta tacctccctg aagaggatgc tctggctcct gcctcacccg gcgacccgga
     3361 cgctgggccg gagggtgggc gacctcggcc acctcctcga ccgccgtcac cgcgaggtgg
     3421 cgctacgcaa cctggcgctc gccctgccgg agctgtccga agcggagcgc cggcgcctgg
     3481 tgcgcgagtg cttccggcac ttcggctccg ccctctgcga cgccatctcc tccacccgct
     3541 tcgacgcggt cgagctctgc cgccgcttcc acttcgaggg gtgggagaac ctcgaggccg
     3601 ccgaggcacg cggcaagggc tcgttcatcc tctcggccca cctcggcttc tgggagatgg
     3661 tgccggcggt catcggcctc taccgcgggc cgatgcacat cgtcgtgcgg ccggcggaca
     3721 acccgcacct cgaccgggag ctcgcggcgc tgcgcgagcg cttcggccac gaggtggtcc
     3781 ccaagcgcgg cgcggcgcgg cggatggtcg aggtgctgcg ccgggccggc cgggtcggca
     3841 tcctcatcga ccagcgggtg cagaggaaag aggggatcga ggtccccttc ttcggccgcc
     3901 cggcgctgac cagcccggtg ctcgccaagc tctccctgcg taccggcgcg gcggtgatcc
     3961 cggtgaccgt ctaccccgga ccggagggca cctggcgctt cgtcgcccgc cccgccatcc
     4021 tgccgccgga aggggtggag gacacccccg aggccgtcgc cgccctcacc cgccgctatc
     4081 tcgaggatgc cgaagaggac atccgctccc accccgaaat gtggctctgg atgcaccgcc
     4141 gctgggaaga gtcccgggcg gccaacccga agtagcccac cttctcctct ggggtgaggg
     4201 gatttttacc tcctcctcgt ttcagcaagt gcggggccgc gatgctgcca caaggggtgt
     4261 gagagacacc cggccccgct tcctgaaaca gtttcggatt ccccgaagga ggagaccgtg
     4321 aagatcacga gagggagttt ctttgtcttt ctgttcgctt tgaccgtgac cctggccggc
     4381 ccggccgtct tgaaggcgga gaccgccgac gcctgcgcgt tcggcttcga gggggcgatc
     4441 ggccccgacc actggtggga gatctgcgcg ccgaagaacc tgacctgcgc cgcgggccag
     4501 cgccagtcgc cgatcgacct gccggcggcg ccagccggcg acctgctcga gctgcgcttc
     4561 ggctatcacc cgacgacgct cgaggtggag aacaccggca ccaccatcga ggtgccggtc
     4621 gagcccggca ccagcgtgct caagatcggc aaggaggagt tcgagctgct ccagttccac
     4681 ttccacaccc cgagcgagca ccggctccag ggcgaggagt tcccgatgga gctgcacttc
     4741 gtccaccgca acgccttggg cgagctggcc gtggtcggcg tcttcctgaa ggagggcgcg
     4801 gcgaacccgg tgatccagca gatctgggac cacgctccca cggcgccgca caccgtctcg
     4861 gaggaggccg gcgagatcga ccccgaggac ctgctgccgg tcgaccgcgg cttctaccgg
     4921 tacgccggct cgctcaccac gcccccctgc agcgagggtg tccggtggca cgtcctgcag
     4981 acaccggtgg agatctcggc ggcccaggtc gacgaattcc gcgccatctt cccggtgaac
     5041 gcccgtcccc tccagctcct caacggccgc ccggtgttga ccgacggcct ctgatcgaac
     5101 ccgctgatcc gtctgacgcg aggggggccg cgaggccccc tttcgcgtgt caccagggat
     5161 cgaagcagtc gtagttgcgt tgggcgacga tgcggccgtc ccgaaactcg aggaacacag
     5221 cgaagcgggc gcgcatctct cctcccaccg ggatcgattc ataggggacc gccagcgttc
     5281 ccgtccactg cacctccagc acgatgttgt cgccctgggc cacggtgttg atcacttcgt
     5341 agcgctggga ggtcatcacc ttccggccgc gggcgccggc atcgaggatg tcctggaggt
     5401 tgcgccgggc gccgttcggc atcagccggt tgggaaactc gtactgaaca tagtccggcg
     5461 cgaagaacga cgccatctcc tcggccggcg cgtggcgctc gatcgattcg aggtagcgcc
     5521 gcgcggtggc gacgttggcc tgctcgtgat cggtcatgat cgggctcctt gatcgttcat
     5581 aggaaatgga cggcgaggcc ggtgattgct ccgagggcgg tccccgcgat cagctccacg
     5641 ggtcgatggc gcgcgagttg gaggcgcgac caggccagcg ccgggatcac cgggaccagg
     5701 caccagccca cgatcgagcc gagcaggacg aggacggtca cggtcaggca ggcgaacgcc
     5761 atgtggagtg aaactttgat ccaccgattg gccaccgcgg cgaccaccac gagggccagc
     5821 gtcccgccga cgccgcggag gagggaggcc tgggtctccc gaagccagag atagaggaag
     5881 agggcgaaga gcgacaggat cgccacggcg aagagggccg gccgctcccg cggctcggaa
     5941 gcgtcgacgt tcccccaccg tccggcccgc acctggcgcg ccatgagcag cgccaccggc
     6001 aggatcacca gtcccaccgc caccgccgcg cccaccccgc cgcgcagcgc gccgaagcgg
     6061 atcccggtgg tgaccgccat gacgaccgcc atcacgaacg gatgcgcgag gatcgagatc
     6121 cagcgggcga gggaggtcga ggccgccatc gaaggggagt ctagcggaag ggtagaatcc
     6181 tgcgtcgaag gccgggcgga ctcctggcaa ccactatcca ccggacgacg agcccgaggc
     6241 cggggctcca tgtcctgtac gtctacgggc gcccgtcgag cttcatcctg cgcctgccgg
     6301 gacgagttga ggcagctcac cttccggcag cgaggaacag ctggtgttga cgttcaagcc
     6361 tgcttggaca agcgatatgc cccaggcaat gcttcctacc aggatattgg ccacttcggc
     6421 ctggggtgcg aagatccaga taatgacctt ctttgccagc cacactagcg cgaaccagcc
     6481 tagcgttgag atgagagact tgatgatcgg catcaacagc ccttgattgt acatctggat
     6541 cagcagctca ttcaccagag taaagagcgc ggccgacatt tttggattgc tttgaatgag
     6601 cgagacgaca gcctgggcag ctgagtagac cgtggcattc ccgacaatcg cattatagat
     6661 ggtagactgc attgccgcat tggcaattga cggcaatatg ccgaccgcca tgaggaggcc
     6721 gcccgtacac tgccacaaga gtgtgatcca tgccaaagtg cagggatcca actgggaaat
     6781 gtcgaccctt gtaacgatca cctcttcggg caaggagaag cgtgacagat ccgcgcccgg
     6841 agccatggcc tccggaacga cgtcgaaagc agcccctggg aagaattggc tcggctgaag
     6901 caccagtgct ccctggctag agctgggatt caacgagagg tagctcccat cctggaaacg
     6961 aatgtatgtc agctcatcgt tgaacaacat gaaggacgcc gaccacgaag aagccgcggt
     7021 ggtgaacacc ggctcgccaa aggcttcgcc aaggtacagg acgctgccat ccggtgcggt
     7081 cgtggtgatg acattgtctg agccgatcgt aaagaccgca gccgagtcgg tcgcttgaac
     7141 cgatacgatg gcgtacgtct gttcatcgag ttgcagccca acggcgacgt actggccggc
     7201 agacgaagca aggtagaacg ttaccgtaga aagaaggctg ttctgattga atacctgtaa
     7261 cgttgcaggg tcggcggcat tgaatagcca ctgatgaaac tcatcgacaa cctgcgcagc
     7321 atccaggacg agccccgtgc cttgcgcagt gagataagtt ccatacaggt tgttgtagag
     7381 aaagcagggg ccgacgttcg cacttccacc tggagttacc aactgccaga tctgcgcctc
     7441 ggaaccggaa taggcaccca tcgagacctg tgctccggtg gcatcgatgc cgatgcatgc
     7501 attggtggaa gcgtttgcaa ggcagaactg atcttctccg cggtagatga gctgccactg
     7561 ttgagtggag attcctgtcg gtgcatccag aaccagagca ccactggcga catctattac
     7621 ggttgacgta ccccaattgg taatcgcgta cgaagtggta gtggccgata cgacaaaatc
     7681 agaaattggc atcgcttcct ctacgagtca tagtagttgt catcacacga ggacgagacc
     7741 ggcatttcta cactgaagcc ttgcgccggc ggcaggaggt tcaggttcac gggctgcggc
     7801 gcggaaaggt cctccgtctc cacgtcgcaa cgctttctgt tcctgtagta agtcacccca
     7861 ttgaggttga attggagcgt ccaatagtca ccgctcccgg agccgccggt gaaattgaag
     7921 ctgatctggt cgttgaggtt catggtatcg acagagaccg agccatccgg cgaatctcca
     7981 aagaactttt ggatgctcac gctcgtgatc agattacccg tttgatttgt caccacacac
     8041 gtatggctca ctccaatacc cctttctcgt agcaatcccg gcaagacaag cacctggcgg
     8101 cgtgcgcgag gtgcggcttc gcagccatcg aggctacccc tggcgatcgc accggtactc
     8161 tactgtttgc ggagggatga tagagctgaa gaagcggagc tgtcaagcgg tggaagctgc
     8221 catcgagagg gagtctggcc gcgggggtag aatccgaccg cgccggactg gcgttattga
     8281 ctggaggagc cctgatgaag aaatcaacga agaagctcgt gctggccaag gagaccgtgc
     8341 ggaacctgga ggagcgcaag ctcggaaagg tgtgcggagc cgccacctat acgtgtggct
     8401 acagctgcgg ccccctgcag tgcccgccgg ccccctacac ccgcgactgc tgaacctcct
     8461 gcccgccccg gagcccttcg acgagcatcg aagtgcttcg ggtccgcctt caactcacag
     8521 ggcggggcgc ccgagcaggc ctgttcagaa gggtgaatcc cggatgagcc acgcatcgag
     8581 catcgtcacg gtacgctcgt catagtgata cagcacccgg atacggacac cgtcgagagg
     8641 cttggtgatc caggttccaa actcgctggg agagtagctc cgtagagcca ggcccagctc
     8701 cgggcgacgg ctcagaaagc tctcgacctg ttgcagggct tctgtggccc gaaggattct
     8761 gtgcgagagc tgctcgctcg cacgcaggta ctcaggctcg tagacgactt ctcgctgcgg
     8821 atcgcgagaa gaatcgctcg atggccaccc gggcgcgctc ttcttcttca tcggttagag
     8881 caggcatctt ctcgggatct tccacgagag ccccctcagg ccagagctct ccagtgacct
     8941 catccgccag gttggcatcg cgcatcttgt gattcagctc gttcggcatg ctgatcctcg
     9001 ccattcctga catcccggtg atctgcgact tcctgccata cacacgatat cgaggtccgg
     9061 tcccgggcgt caagtggcat ggcagggggc aatcgaattc cccacttccc tcttgacgcg
     9121 ccctgagtcc cggtataggc ttcgaggcgg tcgtaagccg tacaatagga aggaggtggc
     9181 aggggagagg aggaagccgc ggttttaaag cacagccgta caggatcgct gtgtccccag
     9241 aaggtgagcg gcgtcgctca tccgggatga cagcgggtcc aagcggccaa gggggagagt
     9301 tggcgctcgt cccccgaggc ctcgccggac aggagagtgt actcatgccc agctacagtg
     9361 atcgtacgcc ttcgcgcgtc gatctgtccc gccccaccga atccacccgc cgcgatctct
     9421 acgagctggt ggtccacgtc tacttccatc ccacgcctga agaggtggag aaggcccggc
     9481 aggaccccgg caaccactgg gtgccggact acggtcccga cgaggcccgg ctccacgtct
     9541 tctacgccta cggccgctgg ttcgcggtct gggaagcgct ggaggagaag aaggagaccg
     9601 gaccgctccc cgagtggcgc cgctggtcgg tggtgcggat cgtcgaggac cgggatccgt
     9661 cgagcgagac gggcgtcacc tttctggagg tctgacgccg tctacctggc gggccccgcg
     9721 gcgcggggtc cgccgccttt ctccgggatc acaatccgcc cttcccggag cgctatcccc
     9781 acctcccgga acacaattcc atggcgccgt atcacttccg gggcaggaag agtcattcgg
     9841 gagcgttccc ggaacgctat tcctgcaccg gagatcattc ccggagcagg aatggccgcc
     9901 ggggaagcgg agagcgtgag cgttcccggt cccgttgtga tccgggaagc ttccctagtg
     9961 ttccgggaag cctcatttgt gttccgggag tgggttgaag gcgcagtttg tgttcccgga
    10021 acactccggg agcactgcgt gacctcgcga tacgcacctg agacggggct cagcgttcac
    10081 ctgtggagga cttcgcgctc gatccgctgc cgaacatcct cccacgggat cccttccgac
    10141 tcaccggaaa ggactctcgc cgcgcgcctc tcgatttccc gcgcccaggc ctcctcggct
    10201 ccgggctccg gctccccgtc caagctggcg aggatctccg aggccagctc cgcacgctcg
    10261 tcgagcggga gctgtaacgc ttgttccaga aggtgatgac gcagggattt catgcaggaa
    10321 gcctagcacc caggtcctac ggcaactcca ccgtcccctg cggggtccag gggccgcagc
    10381 gggcgatgcg ccaggcggac ttggcgactc ctttgacggt gccgtacttg cggatggcgg
    10441 cctcgctgta gtggctgcag gtcggttgga aacggcagcg gactcccacc ttgggcatca
    10501 tgggcgaggc ggtcgcctgg tagaggtcg
//
LOCUS       DASPJI010000564         7480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_36532, whole genome shotgun sequence.
ACCESSION   DASPJI010000564 DASPJI010000000
VERSION     DASPJI010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7480)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36532"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="VEL74_15770"
     CDS             complement(<1..1300)
                     /locus_tag="VEL74_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYG64034.1"
                     /translation="MSSEAVPQRGSAASPASCPAEETEWLGWFDQLADMSAEERAARL
                     DRLAGEQPELADRLARLFAAEEGAPPLLSRPVAERAPELVAAALAPAPGGPDGPALQA
                     GPYRLLKLLGRGGMGEVYRAERADGVFEQQVALKLVRRGMDSEEIVRRFHRERQILAR
                     LAHPGIARILDGGTAPDGRPYFVLELVDGEPILAYCRRHGSPLEERLRLVIDACDAVD
                     SAHRNLVVHRDLKPSNLLVSTGGQVKLLDFGIAKLLANDMSETATRQEDRLLTPAYAA
                     PEQILGEPVTTATDVYALGVILFELLAGRSPHTRFRSPQVVAKEGDTVERPSAVLQDR
                     RFARRVRGELDTIVLAALRRDPARRYRSAADLAADLRRFLEGRPLQVRPDSLLYRTRK
                     LVLRHKLATVALAAAVAGLVGGLGVAVREAEIARAEARRAE"
     gene            1483..2427
                     /locus_tag="VEL74_15775"
     CDS             1483..2427
                     /locus_tag="VEL74_15775"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HYG64035.1"
                     /translation="MTLGKPRVNRLFGQFGCALTIDVEEWYHTCLVPDYVDPEQRPAL
                     RDELDRLLPELLDTLAEAGRSATFFVLGEVAERLPRRVREIADAGHEVGSHGYLHFRA
                     CDRSPEAFRSDLERSKKLLEDVTGTRVLGFRAPEWSLRSLASPCLPLVAEAGFLYDSS
                     LAPYPLSGTTTNPRLASRLTWDRPGRARELLEIPPLTFGPYLRIPTGSWPGRLVRPSW
                     VADAAEEHLEEGGLPVVVIHPWEVSGRPTPGRLKGFAWFCHETGRLGFQARFQELLHA
                     LPWSSIRDAMDLGQSGRKLARSRGAMTSTGATAARLVG"
     gene            complement(2436..3089)
                     /locus_tag="VEL74_15780"
     CDS             complement(2436..3089)
                     /locus_tag="VEL74_15780"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HYG64036.1"
                     /translation="MEQVAVIGLGRFGFHVAKELHLAGHDVLAIDIDAGHVQRIKDFS
                     SRAVVLDARDRQRLEELGIRDFNTVVVSLGERIDASAIIALHLRELGIRRIITKAGSE
                     DHAKLLDLIGVHEVISPEREAAERLAHRLASSNILDFIPMDEEFSVHEIAPPQSFIGK
                     TLEELRLRNRFGVQVLGVRDVLTQHLHVNPDAGFRVKDSDSLLVLGRNQDLDRLDKA"
     gene            complement(3094..4446)
                     /locus_tag="VEL74_15785"
     CDS             complement(3094..4446)
                     /locus_tag="VEL74_15785"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HYG64037.1"
                     /translation="MRAAGPGPLRLLIGSFLAAILCGTMLLRLPLATPPDQRIGWLDA
                     LFTATSATCVTGLTVRDTGTGFTPFGQGVILGLIQVGGLGVMTFSLFILALLGGRVSL
                     AQRSIFERTIGGAASHQVRPLLRLVFLFTFGCEAAGALILFLRWWPEMGAARALWHSV
                     FHSISAFCQAGFFLRPDSLAAWRSDPLVVLPVSILILLGGFGFFTLYDAAQAARTGSR
                     SRLSVHSKLAFVVSAVLVVLGTMAIWVLEAGRAFAGMGPGEQLLAAYFQSVTTRSGGL
                     TTVDIATLGPGTLFLMIILMAIGGSPGSCAGGIKTTTLGVLVVATVSRLRGHRHVNVF
                     RRTLGPETVQNTLTLAQVGLAVALAGAFALLLMETHGRYGGDEPAAFIAYLFETVSAL
                     GTVGLSTGVTPGLSPAGKLLVAFLMFCGRVGPLTLAAALASGRGTGDWQYPEEEVMVG
                     "
     gene            complement(4509..4595)
                     /locus_tag="VEL74_15790"
     tRNA            complement(4509..4595)
                     /locus_tag="VEL74_15790"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4559..4561),aa:Leu,seq:cag)
     gene            4649..6997
                     /gene="rnr"
                     /locus_tag="VEL74_15795"
     CDS             4649..6997
                     /gene="rnr"
                     /locus_tag="VEL74_15795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HYG64038.1"
                     /translation="MSTTFPGMEDLFDEKVLGRLDEAGKKGLAPDQIRRRLDLELTPD
                     ELTATLARLEKRGKALEGNGRWFAMWVTEDAGWIVGTAGLLESGDFIVRTPGRPGEGT
                     ALFVRRRDAKQAMEGDLVLARPMEYRNRKRPRAGDHTKWLPEGLVLKVLSQRHPTLVG
                     ALETGQSGRRWLAPFDPKMNLELQVVGAEGVPDGHYVVVEVERPGNEPSALGRVIEVL
                     GHPDTPGADVLVVLRHHKIPEDFPTAVTEAAEAFPPDPGPEDWAGREDLRKLVTFTID
                     GAKSRDFDDAVSIERLQGGGFRLGVHIADVAHYVQEGTALDLEAYRRGTSVYYPERAV
                     PMLPEGISNGLCSLRPKVPRLTMTVFIDFDRNGNVRGQRFAETVIQSARRLTYDEVRR
                     LLLEPRETDAKRYGRVLPALQDMHELMRVLNRVRVKRGSIDFDLPEGDVQLDTDGVVV
                     GVLATERNVAHRLIEEFMIAANEAVALELESHEVPALYRVHDSPSPDKLMELRELLAD
                     LGFQLKGKLQDLHPSALQKVLIDVHGHPEEAFVSSAVLRTMSRAIYSEECRGHYALAS
                     RYYTHFTSPIRRYPDLVVHRQLKSHLRGNAHAESERTRIAERLPAMGEHTSTTERRAE
                     QSERDLLQWKKVRFLGSRIGEKFEGRITNVQPFGMFIQLLPYYVDGLLPVRSLLGDFF
                     RYEPEGHRLVGDRTGTVYKIGDPIEVVLAGVNLRHRSLDLELPDQPKPEEVDGPWWLR
                     TLYEPPPARPAGKRNSPATKPLPKKGAKKPHKKGTTRTKRRY"
     gene            7075..>7480
                     /locus_tag="VEL74_15800"
     CDS             7075..>7480
                     /locus_tag="VEL74_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYG64039.1"
                     /translation="MSREIGGIHHVTAIASDPQANVDFYTDVLGLRLVKKTVNFDDPG
                     TYHFYYGDEQGNPGTILTFFPWPRARRGSHGAGLTSATSFSVPEGSLGYWTERLARYG
                     VPFEEPRPRFDEEVVTLLDPDGLKLELVARSGD"
BASE COUNT         1170 a         2618 c         2672 g         1020 t
ORIGIN      
        1 gctcggcccg ccgcgcttcg gcgcgggcga tctccgcctc ccggacggcc acgccgaggc
       61 cgcccacgag gccggcgacc gccgccgcca gcgctaccgt ggccagcttg tggcgcagca
      121 ccagcttgcg cgtgcgatag agcagggagt ccggccgcac ctggagaggc cggccctcga
      181 ggaagcgccg gaggtcggcg gcgagatcgg cggcggagcg gtagcggcgc gcggggtcgc
      241 ggcgcagcgc ggcgaggacg atggtgtcga gctccccccg gacgcggcgg gcgaagcgcc
      301 tgtcctgcag gacggccgaa ggacgctcta cggtgtcgcc ctccttcgcc accacctgcg
      361 gcgagcggaa gcgcgtgtgg ggggagcggc cggccagcag ctcgaagagg atcacgccca
      421 gcgcgtagac gtcggtggcc gtggtcaccg gctcgccgag gatctgctcc ggcgcggcgt
      481 aggccggcgt gaggagcctg tcttcctggc gggtggccgt ctccgacata tcgttcgcca
      541 ggagcttggc gatgccgaag tcgaggagct tgacctgccc gccggtgctc accagcaggt
      601 tcgagggctt gaggtcgcgg tggaccacca ggttgcggtg ggcgctgtcc acggcgtcac
      661 aggcgtcgat caccaggcgc aggcgctcct cgagcggtga gccgtggcgc cggcagtagg
      721 cgaggatcgg ctcgccgtcc accagctcca gcacgaagta ggggcggccg tccggcgcgg
      781 tgccgccgtc caggatgcgg gcgatgccgg ggtgggcgag gcgggcaagg atctgccgct
      841 cgcggtggaa gcggcggacg atctcctccg aatccatgcc ccgccggacc agtttcaggg
      901 ccacctgctg ctcgaagacg ccgtcggccc gctcggcgcg gtagacctcg cccatgccgc
      961 cacggcccag gagcttgagg agccggtagg gtccggcctg gagagcgggg ccgtcggggc
     1021 caccgggtgc cggcgcgagc gccgccgcca cgagctccgg cgcgcgctcg gccaccggcc
     1081 gggagaggag cgggggcgcc ccctcctcgg ccgcgaagag ccgcgccagc cggtccgcca
     1141 gctccggctg ctcacccgcc agacggtcca ggcgggccgc ccgttcctcg gcggacatat
     1201 ccgcgagctg gtcgaaccag cccagccact ccgtctcctc cgccgggcat gaagccgggg
     1261 aggccgcaga gcccctctgt ggcaccgcct ctgagctcac tcctgctgtc tcctggcctt
     1321 ggctcctagt ctacgctctt tggagaggtg tgactccggg tgcggattcg tttcaggggc
     1381 ggggctccgg cacgagatgt gcagaaaaac ctgcccatcg ccacgccccg gtgcagcatt
     1441 ccgggtccat ctatgatgcc gttcgacagc ggggagtgtt gaatgacgct cggcaagccg
     1501 cgggtgaacc gtctttttgg ccagttcggc tgcgccctga ccatcgacgt cgaggagtgg
     1561 taccacacct gcctggtgcc ggattacgtg gacccggagc agcggccggc gctccgtgac
     1621 gagctggacc ggctcctccc cgagctgctg gacaccctgg ccgaggcggg ccgctccgcc
     1681 accttcttcg tcctcgggga ggtcgccgag cgcctgcccc gcagggtgag ggagatcgcc
     1741 gacgccggcc acgaggtggg cagccacggc tatctccatt tccgcgcctg tgaccgctcg
     1801 cccgaggcct tccggagcga cctggagcgt tccaagaagc tgctggagga cgtcaccggc
     1861 acccgcgtcc tgggcttccg ggcgccggag tggtcgctgc gcagcctcgc cagcccctgc
     1921 ctgccgctgg tcgccgaggc cggcttcctc tatgacagct ccctcgcgcc ctatccgctg
     1981 tcggggacga cgaccaaccc gcggctggct tcgcggctga cctgggacag gccgggacgc
     2041 gcccgcgagc tgctggagat cccgccgctc accttcgggc cctacctgcg catcccgacc
     2101 gggagctggc ccggccgcct cgtccgtccc tcctgggtgg cggacgcggc cgaggagcac
     2161 ctcgaggagg gggggctgcc ggtggtggtc atccacccgt gggaggtctc cggccggccg
     2221 acgcccgggc gcctcaaggg attcgcctgg ttctgccacg agacgggccg tctcggcttt
     2281 caggcccgct tccaggagct gctccacgcg ctcccctgga gctccatccg cgacgccatg
     2341 gacctcggcc agagcggccg caagctggcc cggagccgcg gcgcgatgac ctcgacgggt
     2401 gccacggcgg cgcggctggt ggggtaggag cgctctcagg ccttgtcgag acgatccaga
     2461 tcctggttcc ggcccagcac cagcagcgag tcggagtcct tgacgcggaa gccggcgtcg
     2521 gggttgacgt gcaagtgctg ggtcaggacg tcccggacgc ccagcacctg cacgccgaag
     2581 cggttgcgca gccgcagctc ctccaacgtc ttgccgatga acgactgggg aggggcgatc
     2641 tcgtggacgc tgaactcctc gtccatgggg atgaagtcga ggatgttgga gctggcgagg
     2701 cggtgcgcca gacgttccgc cgcctcccgc tcgggcgaga tgacctcgtg caccccgatg
     2761 agatccagca gcttggcgtg gtcctccgag ccggccttgg tgatgatccg ccggatcccc
     2821 agctcgcgga ggtggagggc gatgatcgcc gaggcgtcga tgcgctcgcc caggctcacc
     2881 acgacggtgt tgaagtcgcg gatgcccagc tcttccaggc gctgccggtc gcgcgcgtcg
     2941 aggaccaccg cgcgggagga gaagtccttg atccgctgga cgtggccggc gtcgatgtcg
     3001 atggccagca cgtcgtggcc ggcgaggtgg agctccttcg ccacgtggaa gccgaagcgc
     3061 cccaggccga tgaccgcgac ctgttccatg ccgctaacct accatgacct cttcttcggg
     3121 atattgccag tcgccggtgc cgcggccgct ggccagggcc gccgccaggg tgaggggccc
     3181 gacccggccg cagaacatga ggaaggcgac cagcagcttg ccggccggcg acaggccggg
     3241 ggtgacgccg gtggagagcc ccaccgtgcc cagcgccgag acggtctcga agaggtaggc
     3301 gatgaaagcc gccggctcgt cgccgccgta gcggccgtgc gtctccatca gaagcagggc
     3361 aaacgccccg gcgagggcca ccgccaggcc cacctgggcg agggtcaggg tgttctggac
     3421 cgtctccggc cccagggtcc ggcggaagac gttgacatgg cgatgccccc gcagccggct
     3481 gacggtggcg acgacgagca cgcccagggt ggtggtcttg atgccgccgg cgcaggagcc
     3541 gggcgagccg ccgatcgcca tcaggatgat catcagaaag agggtgcccg gcccgagggt
     3601 ggcgatatcc acggtggtga ggccgccgct gcgcgtcgtg acgctctgga agtaggcggc
     3661 gaggagctgc tctccgggac ccatgccggc gaaggcccgg cccgcctcca gcacccagat
     3721 cgccatcgtg cccaggacta cgaggacagc gctcacgacg aaggccagct tgctgtgcac
     3781 cgacagccgg gaccggctcc ccgtacgcgc cgcctgggcg gcgtcgtaga gggtgaagaa
     3841 gccgaaccca cccaggagga tgaggatcga caccggcagc accaccagcg ggtcgctccg
     3901 ccatgccgcg aggctgtccg gccgcaggaa gaagccggcc tggcagaatg ccgagatgga
     3961 atggaagacg gagtgccaga gagcgcgggc cgcccccatc tcgggccacc agcgcaggaa
     4021 gaggatgagc gccccggccg cctcgcaccc gaaggtgaag aggaagacca gacgcagcag
     4081 cgggcgcacc tgatgcgacg ccgctccgcc gatggtgcgc tcgaagatgg agcgctgggc
     4141 cagggagacg cgcccgccca ggagcgccag gatgaacagg gagaaggtca tcacccccag
     4201 gccgccgacc tggatcaggc cgaggatcac cccctggccg aacggggtga agcccgtgcc
     4261 cgtgtcgcgc accgtcaggc cggtgacgca cgtggcgctg gtcgccgtga acagggcgtc
     4321 gagccagccg atgcgctgat cgggcggggt cgccagaggc aggcgcagca gcatcgtgcc
     4381 gcagaggatg gcggcgagga agctgccgat caggagccgc aacgggcccg gtccagcggc
     4441 tctcaccggc tcctgcgact cgaaggccga ttcgtactaa gctctacggg attcgagaga
     4501 ggggggtatg gtgcgaaagg ggggactcga acccccacgg ccttgcgacc acaagatcct
     4561 gaatcttgcg cgtctaccaa ttccgccact ttcgcatggt gcgccgctcg cgcggcgcgg
     4621 agcagattct aaggagacaa cctagaccat gtcaaccaca ttcccgggga tggaggatct
     4681 cttcgacgag aaggtcctcg gacgcctcga cgaagcaggg aagaaagggc tcgcgcccga
     4741 ccagatccgg cgccggctgg acctggagct cacaccggac gagctgaccg ccaccctggc
     4801 gcggctggag aagcggggca aggccctgga aggcaacgga cgctggttcg ccatgtgggt
     4861 gaccgaggac gccggctgga tcgtcggcac cgccggcctc ctcgagtccg gggacttcat
     4921 cgtccgcact cccgggcgcc ccggcgaggg aaccgcgctc ttcgtgcgcc ggcgcgacgc
     4981 caagcaggcg atggaggggg atctggtgct cgcccgcccc atggagtacc ggaaccgcaa
     5041 gcggccgcgc gccggtgacc ataccaagtg gctgcccgaa gggctggtcc tcaaggtcct
     5101 ctcccagcgc caccccacgc tggtcggcgc gctggagacc ggccagtcgg ggcggcgctg
     5161 gctagccccc ttcgatccca agatgaacct ggagctccag gtggtgggcg ccgagggggt
     5221 gccggacggc cactacgtgg tcgtagaggt ggagcgcccc ggcaacgagc cgtcggcgct
     5281 cggccgcgtc atcgaggtgc tcggccatcc cgacacgccg ggcgcggacg tcctcgtggt
     5341 actccgccat cacaagatcc ccgaggactt ccccacggcc gtcacggagg ctgccgaggc
     5401 cttccccccc gaccccgggc ccgaggactg ggcggggcgc gaggacctcc gcaagctggt
     5461 gaccttcacc atcgacggcg ccaagtcccg cgacttcgac gacgcggtct ccatcgagcg
     5521 cctgcagggc ggcggcttcc ggctgggcgt ccacatcgcc gacgtcgccc actacgtcca
     5581 ggaggggacc gccctcgacc tcgaggccta ccgccgcggc accagcgtct actacccgga
     5641 gcgggccgtg cccatgctgc ccgaggggat ctccaacggc ctctgctcgc tgcggcccaa
     5701 ggtgccgcgc ctcaccatga cggtcttcat cgacttcgat cgcaacggca acgtgcgcgg
     5761 ccagcgcttc gccgagacgg tcatccagag cgcccgccgg ctcacctatg acgaggtgcg
     5821 gcgcctcctg ctcgagccgc gggagacgga cgccaagcgg tacggccgcg tcctcccggc
     5881 gctgcaggac atgcacgagc tgatgcgcgt cctgaaccgc gtccgcgtga agcggggctc
     5941 catcgacttc gacctccccg agggggacgt gcagctcgac accgacggtg tggtggtcgg
     6001 ggtgctggcc accgagcgca acgtcgccca ccggctgatc gaggagttca tgatcgccgc
     6061 caacgaggcc gtggccctgg agctggagag ccacgaggtg ccggccctct accgggtcca
     6121 cgactcgccc agcccggaca agctcatgga gctgcgggag ctgctggccg acctcggctt
     6181 ccagctcaag ggtaagctcc aggacctcca cccgtcggcg ctccagaagg tgctcatcga
     6241 cgtccacggc cacccggagg aggcgttcgt ctcgtccgcc gtcctgcgca ccatgtcgcg
     6301 cgccatctac agcgaggagt gccggggcca ctacgctctc gcctcccgct actacaccca
     6361 cttcacctcc ccgatccggc gctacccgga cctggtggtc caccggcagc tcaagtcgca
     6421 cctgcgcggc aacgcccacg ccgagtcgga gcgcacccgg atcgccgagc ggctgccggc
     6481 catgggggag cacaccagca ccaccgagcg gcgggcggag cagtcggagc gcgacctgct
     6541 gcaatggaag aaggtgcgct tcctggggag ccggatcggc gagaagttcg aggggcgcat
     6601 caccaacgtg cagcccttcg gcatgttcat ccagctcctg ccctactacg tcgacggcct
     6661 cctcccggtc cgctccctgc tcggcgactt cttcaggtac gagcccgagg ggcaccgcct
     6721 ggtgggggac cgcaccggca ccgtctacaa gatcggcgac cccatcgagg tggtgctggc
     6781 gggcgtcaac ctgcggcacc gcagcctcga cttggagctg cccgaccagc cgaagcccga
     6841 ggaggtcgac ggcccgtggt ggctgcgcac cctctacgag ccgccgcccg cccgtcccgc
     6901 cggcaagcgc aacagccccg ccaccaagcc gctgccgaag aagggggcca agaagccgca
     6961 caagaagggg accacgcgga cgaagcggag gtactaggga acgacgccat cgactctctg
     7021 ccccctggaa tagggtctgg ttttcagggg tgacagagag aaagggaggc agcgatgagc
     7081 agagagatcg gtggcatcca tcacgtgacg gcgatcgcga gcgatccgca ggcgaacgtg
     7141 gatttctata cggacgtgct gggcctgcgt ctggtcaaga agacggtcaa cttcgacgac
     7201 cccgggacct accacttcta ctatggcgac gagcagggga accccgggac gatcctgacg
     7261 ttcttcccct ggccgagggc gcggcggggg agccacggcg ccgggctgac ctcggcgacg
     7321 tccttctcgg tgcccgaggg ctcgttgggg tactggacgg agcggttggc gcgctacggc
     7381 gtccctttcg aggagccgcg gccgcggttc gacgaggagg tcgtgacgct gctcgacccc
     7441 gacggtctca agctggagct ggtggcgcgg tccggtgacg
//
LOCUS       DASPJI010000565         2684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_383604, whole genome shotgun sequence.
ACCESSION   DASPJI010000565 DASPJI010000000
VERSION     DASPJI010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383604"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..750)
                     /locus_tag="VEL74_15805"
     CDS             complement(274..750)
                     /locus_tag="VEL74_15805"
                     /inference="COORDINATES: protein motif:HMM:NF023276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HYG64040.1"
                     /translation="MLVVADTSALVALAACDGLSLLDALFEAVRVPLEVLRECTVQGK
                     AEAERLGLYLQGRVEEVDLSALVIAATGLGLGELEAMALYKKLHADRLLLDDRRARKV
                     AQLNGIEVVGSIGVLLLAKERGLLSSVWSRLEAVERAGVHLSEALITEAMRLAGEV"
     gene            complement(750..1004)
                     /locus_tag="VEL74_15810"
     CDS             complement(750..1004)
                     /locus_tag="VEL74_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HYG64041.1"
                     /translation="MHVTLDIPEQYLLERTPAEMGRRIQLYAALLMFQSGELSAGAAA
                     EFAGVDRFTFATECQRRGIPLIDYPAEDLQRELASLRHSS"
     gene            complement(1073..>2684)
                     /locus_tag="VEL74_15815"
     CDS             complement(1073..>2684)
                     /locus_tag="VEL74_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HYG64042.1"
                     /translation="RGRTGGAATARPRADLPLEERIARAVVEGSKTGLEDDLGAALAD
                     PRWPSPLDVINGPLMAGMNEVGRLFNDNQLIVAEVLQSAEVMKAAVSYLEPHMEKKAD
                     DSGRGKVLLATVKGDVHDIGKNLVDIVLSNNGFTVVNLGIKVPSEQLIQAVREHKPDI
                     IGLSGLLVKSAQQMVLTAGDLSAVGIDTPLLVGGAALTRRFTHRKIAKAYGGLCTYAK
                     DAMHGLKLVERLTDRAQRPDLEREVADLITADDQETAMETSSTGEAAAPAVAVVRRDV
                     PVPAPPDLERHATNLDLDKVWAYLNPQMIYGKHLGLKGSVRQLEAEGDAKFLKLKAVL
                     DGLKEEARQGAMKARAVWRFFPAKAEGNRVTLLDPETGDPAAPAAAWDFPRQSRNGGL
                     CLADYVLDGDHVALFVTTAGEGVRERVEEWKAKGEYLKSHAFAALALETAEAAAEYLH
                     DCLRREWGFPDSPELVLQDKLSARYRGKRYSFGYPACPDLAFQRELFRALQPEEIGVH
                     LTEGDMMDPEASVSALVFHHPDARYFGV"
BASE COUNT          403 a          950 c          898 g          433 t
ORIGIN      
        1 cacggccacg agctggttgc gggcgaggct ccccttctcg aggcgcaagg ccggctcacc
       61 cggcgcgggc gcggccaccg cgggtgcggc ggtccacagc aggacggtca gggcggtcag
      121 gagccgggcg ttcacggggc aaatctagcg cgaggagggc gggggtggat agggggccgc
      181 cccgcctcct cgtctgctct ctgaggtgct cggtaggcga ggagcgggac gagaggctcc
      241 ctcacactcc gaagcacctc cggtttgggt tgactacacc tctccggcca ggcgcatcgc
      301 ctccgtgatc agcgcctcac tgagatgcac tcctgcgcgc tccaccgcct cgagtcgtga
      361 ccacacagac gagagcaacc ctctttcctt ggccaggagg aggacgccaa tgctgccgac
      421 gacctcgatc ccgttgagtt gagctacctt tctcgccctg cgatcatcca ggagcaggcg
      481 gtccgcgtgg agcttcttgt acagagccat ggcttccaac tccccgaggc caagtcccgt
      541 ggctgcgatc accagagccg agagatccac ctcctcgact ctcccctgaa ggtaaagtcc
      601 caggcgttcg gcttcggctt taccctggac ggtgcactcc ctcaacacct ctagagggac
      661 ccgaaccgct tcgaagaggg cgtcgagcag agagagaccg tcgcacgcgg cgagagccac
      721 gagcgcggat gtgtccgcga caacgagcat caggaggagt gccgcagaga agccagctct
      781 ctctggaggt cctcggcagg gtagtcgatc aggggaatac cgcgcctctg gcactccgtg
      841 gcaaaggtga agcggtcgac tccggcgaac tccgcggcgg cgccggctga aagctctccg
      901 gactggaaca tgagcagagc cgcatagagt tggatgcggc gccccatctc cgccggggta
      961 cgctcgagga ggtactgctc ggggatgtcc agcgtcacgt gcatcgatac acctccttga
     1021 agctcgatga tggagcaaga gccggccgag agcaaccgcg gccggctttc cctcacacgc
     1081 cgaagtacct cgcgtccgga tggtggaaca ccagcgccga gacgctggct tcggggtcca
     1141 tcatgtcccc ctcggtcagg tgcacgccga tctcctccgg ctggagggcc cggaagagct
     1201 cccgctgaaa ggcgaggtcc gggcaggcgg ggtagccgaa gctgtagcgc ttgccgcggt
     1261 agcgggccga gagcttgtcc tggagcacca gctccggcga atcgggaaag ccccattcgc
     1321 ggcgcaggca gtcgtggagg tactcggccg cggcctcggc ggtctccagg gcgagcgccg
     1381 cgaaggcgtg gctcttcaag tactccccct tggccttcca ctcctccacc cgctcccgca
     1441 ccccctcccc cgccgtggtc acgaacaagg ccacgtgatc gccgtcgagc acgtagtccg
     1501 cgagacagag gccgccgttg cggctctggc gcgggaagtc ccacgccgcg gccggcgcgg
     1561 cgggatcgcc ggtctcggga tcgagcaggg tcacccggtt cccctccgcc ttcgccggga
     1621 agaagcgcca gaccgcgcgc gccttcatgg caccctgccg ggcctcctcc ttgaggccgt
     1681 ccagcaccgc cttgagcttc aggaacttgg cgtccccctc ggcttcgagc tggcgcaccg
     1741 agcccttcaa ccccaggtgc ttgccgtaga tcatctgcgg gttcaggtag gcccagacct
     1801 tgtcgaggtc gaggttcgtc gcgtggcgct ccaggtccgg cggcgccggc accggcacgt
     1861 cccgccgcac gaccgcgacc gcgggcgccg cggcctctcc cgtcgacgag gtctccatcg
     1921 cggtctcctg gtcgtccgcc gtgatgaggt cggccacctc gcgctccagg tccggacgct
     1981 gcgccctgtc ggtcagccgc tccaccagct tcaggccgtg catggcgtcc ttggcgtagg
     2041 tgcagaggcc gccgtaggcc ttggcgatct tcctgtgggt gaagcggcgg gtgagggcgg
     2101 cgccgcccac caggagcggc gtgtcgatcc ccacggccga caggtcgccg gccgtcagca
     2161 ccatctgctg cgcgcttttg accagcaggc ccgacaggcc gatgatgtcc ggcttgtgct
     2221 cccgcaccgc ctggatgagc tgctcggacg gcaccttgat ccccaggttg accaccgtga
     2281 agccgttgtt cgacagcacg atgtccacca ggttcttgcc gatgtcgtgg acgtccccct
     2341 tgaccgtggc gagcagcacc ttgccgcggc cggagtcgtc cgccttcttc tccatgtggg
     2401 gctcgaggta ggagacggcc gccttcatca cctcggcgct ctgcagcacc tcggcgacga
     2461 tgagctggtt gtcgttgaac agccggccca cctcgttcat gccggccatg agcgggccgt
     2521 tgatgacgtc gagcgggctc ggccagcgcg gatccgccag cgccgccccc aggtcgtcct
     2581 cgaggccggt cttgctcccc tcgaccacgg cgcgggcgat gcgctcctcg agcggcaggt
     2641 cggcgcgggg tctcgccgtc gccgcgccgc ccgtgcggcc gcgg
//
LOCUS       DASPJI010000566         4241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_603957, whole genome shotgun sequence.
ACCESSION   DASPJI010000566 DASPJI010000000
VERSION     DASPJI010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603957"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VEL74_15820"
     CDS             complement(<1..556)
                     /locus_tag="VEL74_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64043.1"
                     /translation="MSEVPGRSCTEFEELRHRGQQAVEAGRLEEAAALFDQALSRARQ
                     HGEQRLVDLAVCNRAAAFIELDRGEAELPRLREILLRNAEPTSCRIAAYNIARHYELT
                     KNYKKALFYARIAHDRSVVLGRLDWIASSHNLIGNTLLAESHLDQACEQYEQALELIP
                     EEPSAARARILDNLGYCRVLQGRHR"
     gene            complement(537..935)
                     /locus_tag="VEL74_15825"
     CDS             complement(537..935)
                     /locus_tag="VEL74_15825"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HYG64044.1"
                     /translation="MSSENKVRQIQLVGRKIRQLRKEHKLTQVELSSRLGIQQSDLSR
                     MEQGEYRVSLDILFRILAEFQMSIGEFFDGVAQESVTPRDVQLIQSFNALPEDAQREV
                     QDFIEFKRTLGRRDGASHSSEREDDERGTG"
     gene            1081..1845
                     /locus_tag="VEL74_15830"
     CDS             1081..1845
                     /locus_tag="VEL74_15830"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG64045.1"
                     /translation="MRRYLFLLRELVKRDFQGRYAGSLLGFVWSMVQPLWLLVLFTFV
                     FSEVMKATPLGVRTDDFAIFLFCGLLPWLAVQEGVLRASTAITDNASLVKKLRFPSEI
                     LVLAVVLAALLHEAIAALMFLGVLLWVGDLSPGGLPLLLLAVPLQVALTLGLGLLLSP
                     VHVFFRDTAQVLSMVLTAWFYLTPIVYPLPLVPEKFRPWLELNPLTPLVGLYRQAFVG
                     GESVPGVGFLAAAAAVLLSAGFWLFARLKPAFADEI"
     gene            1901..3700
                     /locus_tag="VEL74_15835"
     CDS             1901..3700
                     /locus_tag="VEL74_15835"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYG64046.1"
                     /translation="MSATHTSISFRRFGAYLLAFPLAAALGACSQQPAAAVPADGAQA
                     ASAAPSATPAPPAPDATQPAASPAVSPAEAAVAAPGGESAPGGIEYPQPPDGKWLTDP
                     EGRQYFVHEVPKVERTYMWLDEKQTKVRIGYGETYDVVGHDDKSFRVKMYRVDQPPPP
                     SKADVAADRESVAATYRADVAQADRLMFLPFERGLPDSGQWRNGFEIADMNGDGTLDI
                     VFGSVRKGRRLPNIYLGDGKGGWRLWAEGKFPNLAYDYGDVAVADFNGDRLPDLALGV
                     HLRGILLLASDGPGRFKEWGKGLDFEVPGVAGSAATGFSSRAVVAADWNGDGRPDIVA
                     LGEGPRFAATEAARNSGPGDAFGMVVYLNQGDGSWVRRSDSEKDKIFGDDLAVGDLNG
                     DGRLDAVLGSNVLGERSILRFGEEGGGWRVGSVDVLRPAGYVQSVEMGDFDRDGRVDL
                     LVGYTASEGGVWRSGIDVLYNRPGEKWERRPLAAEEHREGVFALAVGDLDGDRTLDAA
                     ALTGTGATWVFIGDGKGFFRREQGQEITAPVGGCRGYGLQIADLDGDGTGELVSSFAG
                     EASALFAPNRCPQQGGVTAWKAQLNEGEQPAAR"
     gene            4025..>4241
                     /locus_tag="VEL74_15840"
     CDS             4025..>4241
                     /locus_tag="VEL74_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64047.1"
                     /translation="MKKICLALALVSLLGLSGQAFAVINTIDDVPAATLLLPYFEVYL
                     NNANGVTTLFSINNASATAVLAHVTLWT"
BASE COUNT          612 a         1412 c         1492 g          725 t
ORIGIN      
        1 cgcggtgccg cccctggagg acccggcagt agcccaggtt gtccaggatc cgcgcccggg
       61 ccgcgctcgg ctcttccggg atcagctcga gggcctgctc gtactgctcg caggcctgat
      121 cgaggtggct ctcggccagc agcgtgttgc cgatcaggtt gtggctggag gcgatccagt
      181 cgagccggcc caggacgacg gagcggtcgt gagcgatccg cgcgtagaac agggccttct
      241 tgtagttctt ggtgagctcg tagtgacggg cgatgttgta cgccgcgatg cggcagctcg
      301 tcggctcggc gttgcgcagc aggatctcgc gcaggcgcgg cagctcggcc tcgccgcggt
      361 ccagctcgat gaacgccgcc gcccggttgc agacggcgag gtccaccagc cgctgctcgc
      421 cgtgctggcg ggcccgggac agggcctgat cgaacagcgc cgcggcctct tccagacggc
      481 cggcctcgac cgcctgctgg ccacggtgcc tgagctcctc gaactcggtg cagctcctac
      541 ccggtacctc gctcatcgtc ctccctttca ctgctgtggc tcgcgccgtc gcgccggccc
      601 agggtccgct tgaactcgat gaagtcctgg acctcgcgct gggcgtcctc cggcaaggca
      661 ttgaagctct ggatcaactg gacgtcgcgg ggcgtgacgg actcctgggc gacgccgtcg
      721 aagaactcgc cgatgctcat ctggaactcg gcgaggattc tgaagaggat gtcgaggctg
      781 acccggtact ccccctgctc catgcgggac aggtccgact gctgaatgcc gagccgggac
      841 gacagctcga cctgggtgag cttgtgctcc ttgcggagct gacggatctt gcgacccaca
      901 agctggatct ggcggacctt gttctccgag ctcatcgggt gtacgaaatg tatgggtcta
      961 tgtcaaactg acatgcctgg ggtgatggta cggtggcggc gcctccctgt caaccggcag
     1021 ggtggggcgg tccggccccg cgaccggggg ccccggctcg aatcatgctc ggagatctat
     1081 ttgcgccgct acctcttcct cctgcgggag ctcgtcaagc gggacttcca gggccgctat
     1141 gcgggctccc tgctcggctt cgtctggtcc atggtgcagc cgctctggct gctggtgctc
     1201 ttcaccttcg tcttctccga ggtgatgaag gcgacgccgc tgggcgtccg caccgacgac
     1261 ttcgcgatct tcctcttctg tgggctcctg ccctggctgg cggtgcagga gggggtgctc
     1321 cgcgcctcga ccgccatcac cgacaacgcc agcctggtga agaagctgcg cttcccctcc
     1381 gagatcctgg tgctggccgt ggtgctggcc gccctgctgc acgaggcgat cgcggccctg
     1441 atgttcctgg gcgtgctcct ctgggtcggg gatctgtccc ccggcggcct gccgctgctg
     1501 ctgctggcgg tgccgctgca ggtggcgctg accctcgggc tggggctgct cctgtcgccg
     1561 gtccacgtct tcttcagaga tacggcgcag gtgctgagca tggtcctcac cgcctggttc
     1621 tatctgacac cgatcgtcta ccccctgccc ctggtgccgg agaagttccg gccctggctg
     1681 gagctcaacc cgctgacgcc gctggtcggc ctctatcgcc aggccttcgt cggcggcgag
     1741 agcgtgccgg gcgtcggctt cctggcggcc gccgccgctg tgctactatc cgcgggcttc
     1801 tggctgttcg cccgcctcaa accggccttc gcggacgaga tctgacccat ccccaggcgg
     1861 gccccgcctt cgaaggcgaa gacagacaat gaggtttccc atgtcggcca cccataccag
     1921 catttccttc cggcgtttcg gcgcctacct tctagccttc cccctggccg ccgcgctcgg
     1981 tgcctgctcg cagcagcccg cggccgccgt gccggcggac ggtgcacagg cggcgtccgc
     2041 ggccccttcg gcgacccctg ctcctccggc tccggacgca acgcagccgg cggcgagccc
     2101 ggcggtgagc ccggccgagg cagctgtcgc ggcgccgggg ggcgagtccg cacccggagg
     2161 catcgagtac ccccagccgc cggacggcaa gtggctgacc gacccggagg ggaggcagta
     2221 cttcgtccac gaggtcccca aggtcgagag gacctacatg tggctggacg agaagcagac
     2281 caaggtccgc atcggctatg gcgagaccta cgacgtggtg gggcacgacg acaagagctt
     2341 ccgggtgaag atgtaccggg tcgaccagcc gccgccgccg agcaaggccg acgtggcggc
     2401 cgaccgtgag agcgtggccg cgacgtaccg ggccgacgtc gctcaggcgg accgcctgat
     2461 gttcctgccg ttcgagcgcg ggctcccgga ttccgggcag tggcgcaacg gcttcgagat
     2521 cgccgacatg aacggcgacg ggacgctgga catcgtcttc gggtcggtac gcaaggggcg
     2581 gcgcctgccg aacatctacc tgggcgacgg caaggggggg tggcggctct gggccgaggg
     2641 gaagttcccg aacctggcct atgactacgg cgatgtcgcc gtggcggact tcaacggcga
     2701 ccgtctgccg gacctcgccc tgggggtaca cctgcggggc atcctgctgc tcgcctccga
     2761 cgggccgggg cgcttcaagg agtgggggaa ggggctcgat ttcgaggtcc ccggtgtcgc
     2821 cggctccgcc gccaccggct tctcctcgcg cgccgtggtc gcggccgact ggaacggcga
     2881 cggccgtccg gatatcgtcg ccctggggga gggcccccgc ttcgccgcca ccgaagcggc
     2941 ccggaactcc ggcccggggg acgccttcgg catggtggtc tacctcaacc agggggatgg
     3001 gagctgggtg cggcgctccg acagtgagaa ggacaagatc ttcggcgacg acctggccgt
     3061 cggcgacctg aacggcgacg gccgcctcga cgcggtgctc ggctcgaacg tgctggggga
     3121 gcggtccatc ctgcgcttcg gcgaggaggg gggcggctgg cgggtcggca gcgtggacgt
     3181 gctgcggccg gccggctatg tgcaatcggt ggagatgggc gacttcgacc gcgacggccg
     3241 cgtcgacctg ctggtgggct acaccgccag cgagggaggt gtctggcgca gcggcatcga
     3301 cgtcctctat aaccgtccgg gcgagaaatg ggagcggcgg ccgctcgccg ccgaggagca
     3361 ccgggaaggg gtcttcgccc tggccgtggg agacctcgac ggcgaccgca ccctggacgc
     3421 cgccgccctg accggcaccg gtgccacctg ggtgttcatc ggcgacggca aggggttctt
     3481 ccgccgggag caggggcagg agatcaccgc gccggtggga ggctgccgcg gctacggcct
     3541 gcagatcgcc gacctcgacg gcgatgggac cggcgagctg gtgagctcct tcgccggcga
     3601 ggcctcggcc ctgttcgccc ccaaccgctg cccgcagcag gggggcgtga cggcctggaa
     3661 ggcccagctc aacgagggcg agcagccggc cgcgcgatga gctgaaaatg ggctgttgac
     3721 aacgtacccc ttgcggctgc tatagtgcag aagcataatt cacccgagag cagggatccc
     3781 cgaccgtgag cggaagggct gtgagggggt gggggactgc tgctcggccc ggagaaacga
     3841 caagtcgcac cggcggttgt gcctgaagag caacgaggtg cgttaaactt ctggtctctt
     3901 actttttgaa cgggaggtga tgtctcttac tttgtgggga gaggcaaagc agttcaagca
     3961 gtccaacgtg gtcagtactg ctgtattcga gaacgctcaa cgaaagctaa ggagattgtc
     4021 tgacatgaag aagatttgcc ttgcgctggc tctggtgagc ctgctggggc tgagcggcca
     4081 agccttcgcc gtcatcaaca cgattgacga cgtgccggcg gcgaccctgc tgctccccta
     4141 cttcgaggtt tacctcaaca acgcgaacgg cgtcaccacg ctgttctcca tcaacaacgc
     4201 gtcggccacc gcggttctgg cccacgtcac cctgtggacc g
//
LOCUS       DASPJI010000567         6380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_383755, whole genome shotgun sequence.
ACCESSION   DASPJI010000567 DASPJI010000000
VERSION     DASPJI010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383755"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            467..685
                     /locus_tag="VEL74_15845"
     CDS             467..685
                     /locus_tag="VEL74_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64048.1"
                     /translation="MFFIREMIDSGFTWLFAAVILPLCLIVPALCTTASSENPAARTD
                     SMHMRSDSSSIDTPEVLVPKPPPPPPTY"
     gene            complement(753..4049)
                     /locus_tag="VEL74_15850"
     CDS             complement(753..4049)
                     /locus_tag="VEL74_15850"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYG64049.1"
                     /translation="MGQESSPGRIGPYQTLKRIGVGGMGEVFLAYDSRLDRRVAIKRI
                     RPGAVVTPVSRERFRREARLAARLSHSAIVQVYDILEEEGSDYIVMEYVEGTTLRDQV
                     RERPMDVRAALELARELVAGLDTAHRQGIIHRDLKTDNVLISASGGPKILDFGIAKCF
                     LDSEEGSLTAANSILGTCRSMSPEQARGEPVDPSTDLFALGVLLYEALTGTSPFEAEN
                     PVSTLTRLALHRQTPVHKLNPEVPWQLSQLIDRLLEKTPEMRPGSAAEVRRELEEIAS
                     RLGTTSHSGAPDEGTVATDIQMPTLVADLPVPSEPRPAFPRRFHLVLGIALLIGVALG
                     LASWRLRSGARDLSVLTQPVDRLSPAGAGATFAVLRFQDRSATGESSWMGTAFPEMIT
                     DLLAIDGRMSSVPHRDVPRAELDLRLSGREGLADYQRRLDVDYLISGSYEARGDEQLI
                     VRFLVLDRSGGRWLSEVLPGSVPGLLQLSSEIAAGVRRELLLPLPGADGRKAVEAIYA
                     DLPQEAWPGYFAGLGHVYGANHEKAAQLFAETVRLAPRSPLPARALALELRTTGHPAD
                     AARAACRAQELSGLLPAPHDRAEIAALCHELSGRSTEARRLYEALLPEYPLDQAPFRL
                     KIAELNLYDRETDRKNDALNVLQDLRNADPRTLRASDRLSADLIEAEVLVQKESYPEA
                     LSLARAATAQARFLHAFVDRAAARRIEGVSLLQSKDYRGAIAPLQEACTQLEAARRKL
                     KVVHCKESLVLAQFFGDRTPRYDLLADAREAYRAAGNLVDVGRMLQLESVLRQVQGDH
                     AEAERLNREAETLFERIGAHQELANGWALMGSKLLFLGELQEARKVLDQAYAFFSRQG
                     QAASAALVLRNLGRIRFLHGDLRGARQACDVSPSGERVYLLAQVEAAEGRPARAVELL
                     KELLVREEQRNPALTAKTVISLADLRQADGAAGEALELARRAEQILAGSEQRDLIIQA
                     QLQLVKIHLAQKDFAAANERFEAIRERAEERQDYLVTLETGITAARLKGLLGGGRRRD
                     EALDDLATIERDCRQKGNVIYAFEARLAAGELMRPPERGARLEEIAREARRLGLGQIA
                     RRAEKIRREE"
     gene            complement(4063..5700)
                     /locus_tag="VEL74_15855"
     CDS             complement(4063..5700)
                     /locus_tag="VEL74_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYG64050.1"
                     /translation="MSFLRFDGSTLDLSRTGTDVLWGKGNLAFEPIPTLTILFHPQAP
                     RVGARAYLGPIVRGQEARLSRLEPDFEQPGSQAPLNDPYLSRRPIRFLPVPGGIEIRL
                     DEGHTRIALNGEPVDGSRVLSLAEIQEGTVLEMAGRVVLLLHLFVAPGLPVCEPHGLV
                     GESSAIAQVRAEIQRVADLAVPVLIRGATGTGKELVAQAIHAASRRHRGPFVSVNLGA
                     IPPSLAASELFGAARGAYTGAVQAQDGYFRKAQGGTLFLDEIGETPQEVQASLLRVLE
                     TGEIQSVGAQSALRVDVRVLGATDADLEQRIQAGTFREPLLHRLAGYSIRLPPLRERR
                     DDIGRLLVHFLRQELAATGELEILDRDSPHLLWMKPALVARLARYDWPGNVRQLRNAV
                     RQLVIGSRGLPQLQSSPGFESLLGGGLPIQAPVEPPADAPAVSGVVSGPGPAGRSAAA
                     RRRKPAEISQEELLTVLRAHRWSLKSAAEELGISRPSLYVLIERCPAIRKAGDLKVEE
                     IVLCHRECGGDLDLMVECLMVSRDALHRRIRELGLVS"
     gene            complement(5751..>6380)
                     /locus_tag="VEL74_15860"
     CDS             complement(5751..>6380)
                     /locus_tag="VEL74_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64051.1"
                     /translation="LSEMSQWRDLRLQDPRLLEDVSERLMQLAGEQEFAFLYALAHCG
                     KGWALCLRGDLEEGTEMIQTGLDLYAATGARLMRGYWATYLIEAHLAAGRIAEGLAVV
                     REALALSETQLDVTFDPELLRLEGELLYASGEAQAAEAVFRKALEITREQKARAFELR
                     TAISLGRLLAVQGRAEEALPLLSAVEQTLYKGLENRDLMEARELLRQLS"
BASE COUNT         1057 a         2193 c         2086 g         1044 t
ORIGIN      
        1 gtgcaaccgt tccatggaga acctcctgtg gcccgggcgc gccccgggcg gcctacggtg
       61 ggggggatga gggagcgcgg cgcgctccgg cggcccgact acagcccggg tgtcgctgca
      121 aatcgcgcac cgggcttcgg ggaatgagac agcgtcagag ggatctatgg aacaccgtca
      181 cgcccgcgcc cttcaatcca tcttttcagt aaaagacggc gaacacctcc cggcccggcg
      241 gctgctcttg ctgagacggc tcgccgcctc cggctcttga cacgtcaatc gcccccgact
      301 tgacctgtca attatcacgt tttcttctct ttacagcctc tcgacagacc aatctccctc
      361 gggagccggc ctggcacgcc ggctgcaatg ttcggttttt cgaatccgga ttcgatcgag
      421 aaactgaaac gcgaactaaa accgcttcac cagatggagg taagtcatgt tttttattcg
      481 agagatgatc gactccggat tcacttggct gttcgccgct gtcattcttc cgctgtgcct
      541 gatcgtcccg gcgctgtgca ccacggcttc ttccgagaat cccgcagcgc gaactgacag
      601 catgcatatg cgttcggaca gcagctccat cgacacgccg gaagtcctcg tcccgaagcc
      661 cccgccgccg ccaccgacct actaggaatc acggcgatgg agcagacggc ctgatgtagc
      721 tgagatgtgt gagcaccgga cgggaggagg gcctactcct ccctccggat cttttccgcc
      781 cggcgggcga tctgtccaag gcccagccgg cgcgcctctc gtgcgatctc ctccagcctc
      841 gcgcctctct ccggcggccg catcagctca ccggcggcga gccgggcttc gaaggcatag
      901 atcacgtttc ccttctgtcg acagtcgcgc tcgatcgtgg ccagatcatc cagcgcctcg
      961 tctcgccgcc gcccgcctcc gagaagaccc ttcaagcgtg ccgccgtgat gccggtttcg
     1021 agagtgacta gatagtcctg gcgctcttcg gcgcgctcac ggatcgcttc aaacctctcg
     1081 ttcgccgcgg cgaaatcttt ctgtgcgagg tggatcttca cgagttggag ctgagcttgg
     1141 atgatgagat cgcgttgctc gctgcctgcc agaatctgct cagcgcggcg agcgagctcc
     1201 aaggcttcgc cggccgcacc gtcggcctgc cggagatccg ccaggcttat gacggtcttc
     1261 gctgtgaggg cgggattccg ctgctcctct cgaacgagca actccttcag gagctcgacc
     1321 gcccgcgcgg gtcgcccttc cgccgcctcg acctgagcca gcaagtagac tctctctccg
     1381 gacggcgaga catcacacgc ctgccgcgcg ccacggagat caccgtgcaa gaagcgaatc
     1441 cttcccaggt tccgcaaaac cagagccgcg gaggctgcct gcccctgacg gctgaaaaag
     1501 gcgtaggctt gatcgaggac cttccgggcc tcctgaagct ctcccaggaa caggagtttg
     1561 ctgcccatca aggcccatcc gttcgccagc tcttggtgcg ctccaattcg ttcgaagagc
     1621 gtttccgcct cccggttcag ccgctcggcc tccgcgtggt cgccctgcac ctgccgcagg
     1681 acggattcga gttggagcat ccgtccgacg tccaccaggt tgccggccgc acggtaggcc
     1741 tctcgggcgt cggccagcag gtcgtagcgt ggagtccggt cgccgaagaa ctgcgccaac
     1801 accagactct ccttgcaatg gacgaccttg agcttccgac gggcggcctc caactgggtg
     1861 caggcttcct gcaagggtgc gatggcgccg cgataatcct tagactgcag caggctcacg
     1921 ccttcaattc ggcgcgccgc ggcccggtcg acgaaggcat gaaggaagcg ggcctgcgcc
     1981 gtggctgcgc gcgcgagact cagagcttcg ggatacgatt ctttttgaac gaggacttcg
     2041 gcctctatca ggtcggcgct gagtcgatcg ctcgcgcgca acgttctcgg gtcggcgttc
     2101 cgaagatcct gcaggacgtt gagcgcgtcg ttcttgcgat ccgtctcacg gtcgtacagg
     2161 ttcagctccg cgatcttcag ccggaagggg gcttggtcta gagggtactc agggaggagc
     2221 gcttcataga gacgccgggc ttctgtgctc cggccgctga gctcgtggca gagcgcggcg
     2281 atctccgcgc ggtcgtgtgg cgcgggtaga agcccgctca gctcctgagc ccggcacgca
     2341 gcgcgcgccg catccgccgg atgtccggtc gtcctcagct ccagggccag ggcccttgcc
     2401 ggcaaggggg agcgcggcgc caggcgcacc gtctccgcga agagctgcgc cgccttctcg
     2461 tgattcgcgc catagacgtg cccgagcccc gcgaagtagc ccggccaggc ctcctgaggg
     2521 aggtccgcat agatcgcctc cacggccttc cggccgtcgg cgccaggaag cggcaggagc
     2581 agctcccggc ggaccccggc ggcgatctcg ctggaaagct ggaggagccc gggcacggag
     2641 cctggcagga cctccgagag ccagcgcccc cccgacctgt cgagtacgag gaaccgaacg
     2701 atcagttgtt catcccctcg cgcttcgtac gagccggaga tcagatagtc gacgtcgagt
     2761 ctccgttgat agtcggcgag cccttcccgc ccggagagac gcaggtccag ctcggcgcgc
     2821 ggaacgtccc ggtgcgggac gctgctcatc ctcccgtcga tcgccagcag atcggtgatc
     2881 atttcgggga aggccgtacc catccaggag ctctcccccg tcgctgagcg atcctggaac
     2941 cggaggacgg cgaacgtcgc tcccgcgccg gccggcgaca aacggtcgac cggctgcgtg
     3001 aggacggaga gatcgcgggc tccgctccgc aggcgccacg aagcgaggcc gagagccacc
     3061 ccaatcagga gcgcgatccc gaggacgaga tgaaaacggc gtgggaaggc cggacgcggc
     3121 tccgaaggga ccgggaggtc ggctaccagg gtgggcatct ggatgtcggt cgccacggtg
     3181 ccctcatccg gtgcgccgga gtggctcgta gtgccgaggc gcgaggcgat ctcctccagc
     3241 tcccgccgca cctcggcggc cgagccgggg cgcatctccg gggtcttctc caggagccgg
     3301 tcgatcaact gcgagagctg ccagggaacc tccggattga gcttgtgcac cggcgtctgc
     3361 cggtgcaagg ccaggcgggt cagggtgctc accgggttct ccgcctcgaa aggcgaggtg
     3421 ccggtcaagg cttcatacag cagcacaccc agcgcgaaga ggtcggtgct ggggtccacc
     3481 ggctcgccgc gggcctgctc cggcgacatc gagcggcacg tgccaaggat cgagttggcg
     3541 gccgtcagcg agccctcctc gctgtccaga aagcacttgg cgatgccgaa gtccaggatt
     3601 ttcggtccgc cggaggcgga gatcaggacg ttatcggttt tcagatcgcg gtggatgatc
     3661 ccctggcgat gcgccgtgtc cagccccgcg accagctcgc gcgccagctc cagggccgcg
     3721 cgcacgtcca tgggtctctc gcggacctgg tcgcggaggg tcgtcccctc cacgtactcc
     3781 atgacgatgt aatcgctccc ctcctcctcc aggatgtcgt agacctgtac gatggcggag
     3841 tggctcagcc gcgcggccag ccgggcctcc cggcgaaagc gctcgcggct cacgggcgtg
     3901 accaccgcgc cgggccggat gcgcttgatc gccactctcc ggtcgaggcg agagtcatag
     3961 gcgaggaaca cctcgcccat acctcccacc ccgatgcgct tcagggtctg gtagggcccg
     4021 atccgtccgg gagagctctc ctgccccacg tccaagctcg cttcaggaaa ccaggccgag
     4081 ctcgcggatg cggcggtgga gagcatcgcg cgagaccatc aggcattcga ccatgagatc
     4141 gagatcgcct ccgcactcgc gatggcagag cacgatctcc tcgaccttga ggtcacccgc
     4201 cttgcggatc gccggacaac gttcgatcag cacgtagagc gaaggccgcg agatccccag
     4261 ttcctcggcc gcgctcttga ggctccagcg atgagcgcgc agcacggtca gcagctcctc
     4321 ctgagagatc tccgccggct ttctccgccg ggcggcggcc gatctgccgg ccggcccagg
     4381 acctgagaca acaccggaaa cggctggcgc gtcggccggc ggctcgaccg gcgcttggat
     4441 cggaaggccg ccgccgagga gactctcgaa ccccgggctg gactggagct gtggcaggcc
     4501 gcggctgccg atgaccaact gccggaccgc gttgcggagc tgccgcacat tgcccggcca
     4561 gtcgtaccgg gccagccgcg ccaccagagc cggcttcatc cagagcaggt ggggcgagtc
     4621 ccggtcgagg atttccaatt cgccggtggc ggccagctcc tggcgcagga agtggaccag
     4681 caggcgcccg atgtcgtcgc gccgctcccg cagaggcgga agccggatcg agtaccccgc
     4741 gagccggtgc aggaggggct cgcggaacgt ccccgcctgg atccgttgct cgaggtccgc
     4801 gtcggtggcc ccgaggaccc gcacgtcgac ccgcagggcg gactgtgcac ccaccgattg
     4861 gatctcgccg gtctccagca cgcggaggag cgaggcctgc acctcctgtg gcgtctcgcc
     4921 gatctcatcg agaaaaagag tgcccccctg ggccttccgg aaatacccgt cctgggcctg
     4981 caccgcgccc gtgtaggctc cgcgggctgc gccgaacagc tccgaggcag ccagcgacgg
     5041 gggaatggcg cccaggttga cgctcacgaa cggaccgcga tgcctgcggc tggcggcatg
     5101 gatcgcctgc gcgaccagct ccttgcccgt tcccgtcgcg ccccggatca ggaccgggac
     5161 cgcgagatcg gcgacgcgct ggatctccgc gcggacctgg gcgatcgccg agctctcccc
     5221 gaccaggcca tggggctcgc agaccgggag cccgggcgca acgaagaggt ggagcaacag
     5281 gaccacgcgg cctgccatct ccagcacggt cccctcctgg atctcggcca gggagagcac
     5341 gcggctgccg tcgaccggct ctccattcag ggcgatccgc gtgtggcctt cgtccagccg
     5401 aatctcgatg ccgccaggga cggggaggaa acggatcggt cgccggctga gataggggtc
     5461 gttgagcggt gcctgggagc ccggctgctc gaaatcaggc tccaggcggc tgaggcgggc
     5521 ctcctgccct cgaacgatcg ggcccagata tgcccgcgcc ccgacgcgag gggcctgcgg
     5581 gtgaaacagg atggtcagcg tagggatcgg ctcgaaggcg aggttgccct tgccccacaa
     5641 cacgtccgta cctgtgcgag acaggtccag cgttgagcca tcgaatcgca gaaagctcat
     5701 gaatgcgccg tcgagtgaac gcagtctatc atgagccgct gagtcgcccg ttaggacaat
     5761 tgacggagta gctcccgcgc ctccatgaga tcgcgatttt cgagcccttt gtaaagggtc
     5821 tgttcgaccg cggagaggag gggcagcgcc tcctccgcgc ggccctgcac ggcgaggagg
     5881 cggcccaggc tgatcgctgt gcggagctcg aaggcgcgcg ccttctgctc gcgggtgatc
     5941 tccagagctt tccggaagac cgcctcggcc gcctgggcct ccccggacgc gtaaagaagc
     6001 tcaccctcca ggcggagaag ctcaggatcg aacgtgacgt cgagctgggt ctccgacaat
     6061 gccagggcct cgcggacgac agccaatccc tcggcgatcc ggccggccgc cagatgagcc
     6121 tcgatcagat aggtggccca gtagccccgc atcaggcgcg ccccggtggc cgcatacagg
     6181 tcgagaccgg tctggatcat ctccgtccct tcctccagat caccgcgcag gcagagcgcc
     6241 cacccttttc cgcagtgtgc cagggcatag agaaaggcga actcctgctc gcccgcgagc
     6301 tgcatcagcc gttcggacac gtcctccagc agccgtgggt cctgcaaccg cagatcgcgc
     6361 cactggctca tctctgagag
//
LOCUS       DASPJI010000568         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_93860, whole genome shotgun sequence.
ACCESSION   DASPJI010000568 DASPJI010000000
VERSION     DASPJI010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93860"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /gene="pilO"
                     /locus_tag="VEL74_15865"
     CDS             <1..485
                     /gene="pilO"
                     /locus_tag="VEL74_15865"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HYG64052.1"
                     /translation="LPKFREEVRQLELELDKLLRILPARRNTPDLLRRIRTLAEQGDF
                     ALKRFTPGQLSDQEFYSQWPIAISIEGTYHNLALFFDKISRFSRIINVENLEVGAIAR
                     PGNPHTIAASFSAKTFVYKEPEPEEETETATGPAGARKAAGKAAAAQNARTGSAGGEE
                     "
     gene            482..1003
                     /locus_tag="VEL74_15870"
     CDS             482..1003
                     /locus_tag="VEL74_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64053.1"
                     /translation="MIRSLHVLGLVALLLLPAGLATSQEGQQQPAPPAEGAPETGPDL
                     GNIDEILEGEEEVLSGSSFSYDPGNRRDPFKSLLAGPDRQEVRGPRPEGIPGLMIDEI
                     DLTGIFRTSRGFVAQVSAGNQKRSFLLKEGDQLYDGDVVSINRNEVVFKQIVQDPTAL
                     KPFREVVKSLGPS"
     gene            1048..>2188
                     /locus_tag="VEL74_15875"
     CDS             1048..>2188
                     /locus_tag="VEL74_15875"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HYG64054.1"
                     /translation="MGEVVIRRQGAGWPRRTAAAALLAALVIGCTSSKPARVVEATPE
                     TEAASTASAAARTAPAATEIRSLDLREGAPEVYIDLEANGPLVWTSYRNADGRVIVEL
                     PNAVPADAVADLAPEEGLVSSLKVERAEEGSRPLTRLFIAARQDVEHSVTADGPKLRI
                     QLMPTGEMAEEAESRIAFEPVAPAAPAAEPAAPAMEPAPGEEVRAESRAASGTPDEPS
                     VAPAPAGATATRLDRVEVLESAGTSVVRIAGDGEFPYSTFILTEPTRFVIDLAGVINR
                     SAPSTIQVPGGQVERVRVAQYKPLPKPVARVVFDLKSSVVPVIERTADALVVSFPASG
                     DGTIESAGESQAALEPAGTSDLALRPSGPAAAVASAQPRREPAIEV"
BASE COUNT          329 a          774 c          800 g          285 t
ORIGIN      
        1 agctgccgaa gttccgcgag gaggtgcggc agctcgagct ggagctcgac aagctcctgc
       61 gcatcctgcc ggcccgccgc aacaccccgg acctgctgcg ccgcatccgc acgctggccg
      121 agcaggggga tttcgcgctc aagcgcttca cgccgggcca gctctccgac caggagttct
      181 acagccagtg gcccatcgcg atcagcatcg aggggaccta tcacaacctc gcgctgttct
      241 tcgacaagat cagccgcttc tcgcggatca tcaacgtcga gaacctggag gtcggggcga
      301 tcgccaggcc cggcaacccg cacacgatcg cggcgagctt cagcgccaag acgttcgtct
      361 acaaggagcc cgagccggag gaggagacgg agacggccac gggcccggcc ggcgcccgca
      421 aggcggccgg caaggccgcg gcggcgcaga acgccaggac cggctccgca ggaggtgagg
      481 aatgatccgt tctctgcacg tgctgggcct cgtggccctg ctgctgctgc cggccggcct
      541 ggcgacgtcg caggagggcc agcagcagcc cgcgccgccg gccgaagggg ctccggagac
      601 ggggcccgac ctcggcaaca tcgacgagat cctcgagggg gaggaggagg tcctctccgg
      661 ctcctctttc tcctatgacc cgggcaaccg ccgcgacccg ttcaagtcgc tgctcgccgg
      721 gccggaccgc caggaggtcc gcggcccccg tcccgaaggg atcccggggc tgatgatcga
      781 cgagatcgac ctcaccggca tcttccgcac ctccaggggt ttcgtcgccc aggtgagcgc
      841 cggcaatcag aagcgcagct tcctgctcaa ggagggggac cagctctacg acggcgatgt
      901 ggtgagcatc aatcgcaacg aggtggtctt caagcaaatc gttcaggacc cgacggcgtt
      961 gaagccgttc cgggaagtcg tcaagagcct gggcccgtca tgagcgggca ttggatgttt
     1021 tcaggtcccg agagggaggc ctcagccatg ggagaagtgg tcattagaag acagggcgcc
     1081 ggatggccgc ggcggaccgc ggcggcggcg ctgcttgcgg ccctggtgat cggttgtacg
     1141 tcctcgaagc ccgcgcgggt ggtcgaggcg acgccggaga cggaggccgc gtccaccgcg
     1201 tcggcggcgg cgaggacggc cccggccgcg acggagatcc gctcgctgga tctgcgcgaa
     1261 ggggcgccgg aggtctatat cgacctcgag gcgaacgggc cgctggtgtg gacgagctac
     1321 cgcaacgccg acggccgcgt gatcgtcgag ctgcccaacg ccgtcccggc ggacgcggtg
     1381 gccgatctgg ctcccgaaga ggggctggtc tcgtcgctca aggtcgagcg ggccgaggag
     1441 gggagccgtc ccctcacccg gctgttcatc gccgcgcggc aggacgtgga gcactcggtg
     1501 accgccgacg ggccgaagct ccgcatccag ctcatgccga ccggcgagat ggccgaggag
     1561 gccgagtccc gcatcgcctt cgagccggtc gcgccggcgg ccccggccgc cgagccggcc
     1621 gcgccggcga tggagccggc gccgggtgag gaggtccggg ccgagtcccg cgccgcctcc
     1681 ggcacgccgg acgagccctc ggtggccccg gcccccgccg gcgccacggc gacccgcctc
     1741 gaccgcgtcg aggtgctgga gtccgccggc acctcggtgg tccgcatcgc gggcgacggc
     1801 gagttcccgt actcgacctt catcctcacc gagcccaccc gcttcgtcat cgacctggcg
     1861 ggggtgatca accgcagcgc gccgtcgacc atccaggtgc ccggcgggca ggtggagcgg
     1921 gtgcgggtgg cgcagtacaa gccgctgccg aagccggtcg cccgcgtcgt cttcgatctc
     1981 aagagctcgg tggtgccggt gatcgagcgg accgcggacg ccctggtggt gagcttcccg
     2041 gcttccggcg acggcacgat cgagagcgcc ggcgagagcc aggcggccct cgagcccgcc
     2101 ggcacgtcgg acctggcgct ccgcccgtcg gggccggcgg ccgccgtcgc ctccgcccag
     2161 ccccggcgcg agccggcgat cgaggtgg
//
LOCUS       DASPJI010000569         3658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_383958, whole genome shotgun sequence.
ACCESSION   DASPJI010000569 DASPJI010000000
VERSION     DASPJI010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383958"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="VEL74_15880"
     CDS             complement(<1..1107)
                     /locus_tag="VEL74_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HYG64055.1"
                     /translation="MFRRFKLVLPALAAVALAGAGAASAQTKLLRFPDIHGDRVVFTY
                     AGDLWTAPVAGGTATRLTAHPGLEMFAKFSPDGQSIAFTGQYDGDEQVYVVPATGGVP
                     KQLTFYPARGPFPPRWGYDNQVYGWTRDGQEVLFRSLRDGWDLSDSRLYTVPVAGGLG
                     EPLPMPQSGAGEFSPDGKRMVYTPMARDFRTWKRYQGGWAQDLWIFDLASHDAKNITS
                     NPRTDRDPMWIGDTVYFNSDRTGTLNLYAYDPKSGDTRQLTRSTRGDVRWPSADETGR
                     IVYEMEGELAVLDIRSGQSQALRIHVPGDGLGNRPSQVSAANQIEGSSLSPKGERALF
                     VARGDVFTAPIEKGVTRNLTRSSRYHDKLARWSPD"
     gene            complement(1307..>3658)
                     /locus_tag="VEL74_15885"
     CDS             complement(1307..>3658)
                     /locus_tag="VEL74_15885"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYG64056.1"
                     /translation="TIDGVDFNNAFFGGTVGTAEGRAPLSISQESIKEFTVITNGASV
                     EFGRSGGGFVNVITKSGTNNLHGSAFYYDQPQDLISDFPNGTEPAEQEKQQYGASIGG
                     PILQDRLFYFLSYDNQDQSVSVPIDPLLLDQDIFGRYPVLASPDTYSSTQDGSVLFGR
                     LDFQASPSHRFMLRGNFTEYEGINGTSTSNTRTASFNGLEGLNSDSWVASYSSQWGAN
                     LLNDLNINYITEDMPRNDKGLNLPEIQVTLGSISARYGEVAFLPIETTTDRRAIADTL
                     SYSLGRHFLKAGFEYNDTSVDQVFRGNWRGVFIFNNEADLLAGRWTEYRQFGGLNGLT
                     SSEAGRSDFAQEETALFLQDQWFVRPNLTISAGVRWERLDNPDFAILNPNDVDTAAGR
                     LRLTAQIPDSDNQISPRLGISWAPDEKTAVRFSAGRYWSRTPALLWAQTFTSNGIRGT
                     QLTVRRDANGNITNGPADTPSPAWGPGWSPEGIERIDLGRLTRLAAPGVYTVDPDFEN
                     PYTDRATLGIEREIFRLASFGLDFTYAEGKQLQRLTDINRVYDPANPPTPNGLPRYSN
                     TRPLSGYDRVIMNVSDAESEYTAVTATLQRRYADNFSLYGAVTWSEDKDHDSNERNFA
                     GIQSEDFNNLDLNWGYSNRDQRWKGVLNGLWDTPWWGLGLSGSFRYSTGQPFTATTNS
                     DFNNDGVRETDRPTVGGEHFDRNSFRQPDFWTLDMRLSKGFDLGPGELTLFAECFNCT
                     DRTNPFTTRTVWGTAQTPAAGFAGENSWIGNPRTLQLALRYDF"
BASE COUNT          544 a         1096 c         1409 g          609 t
ORIGIN      
        1 gtccggcgac cagcgggcca gcttgtcgtg gtagcgcgag ctgcgggtga ggttgcgcgt
       61 cacccccttc tcgatgggcg cggtgaagac gtccccgcgg gcgacgaaca gggcccgctc
      121 ccccttgggc gagagcgagg agccctcgat ctggttcgcc gccgagacct gcgacggccg
      181 gttccccagc ccgtcgccgg ggacgtggat gcgcagcgcc tgcgactgcc ccgagcggat
      241 gtcgagcacc gccagctcgc cttccatttc atagacgatg cggccggtct cgtcggcgct
      301 cggccagcgg acgtcgccgc gggtgctgcg ggtgagttgc cgcgtgtcgc cgctcttcgg
      361 gtcgtacgca tagaggttga gcgtgcccgt gcggtccgag ttgaagtaga ccgtgtcgcc
      421 gatccacatc ggatcgcggt ccgtgcgagg gttgctcgtg atgttcttcg cgtcgtggct
      481 ggcgaggtcg aagatccaga ggtcctgagc ccagcccccc tgatagcgct tccaggtccg
      541 gaagtcacgg gccatggggg tgtagaccat gcgcttgccg tccggcgaga actcccccgc
      601 gccggactgc ggcatgggga ggggctcgcc gaggccgccg gccaccggca cggtgtagag
      661 ccggctgtcc gacaggtccc agccgtcgcg cagggaacgg aacaggacct cctggccgtc
      721 acgggtccag ccgtagacct ggttgtcgta gccccagcgc ggcgggaagg gtccgcgcgc
      781 gggatagaag gtgagctgct tcggcacgcc gccggtggcc gggacgacgt agacctgctc
      841 gtcgccgtcg tactggccgg tgaaggcgat cgactggccg tcgggggaga acttggcgaa
      901 catctcgagc cccgggtgcg cggtgaggcg cgtcgcggtg ccgcccgcga ccggggcggt
      961 ccacaggtcc cccgcgtagg tgaacaccac ccggtcaccg tggatgtccg ggaagcggag
     1021 tagcttggtc tgtgcggaag ccgcaccggc gcccgcaagc gcgacggcgg ccagcgcggg
     1081 gaggacgagc ttgaatcgac ggaacatggc ggctcctgaa agttgcagaa aatgacaaac
     1141 cccggcgcgc gggcaacacg ccggcggcgc ctgggaccag atctcggctg aggaggatac
     1201 ggcgaaaagg ggtggaggtt gcaggaacgg aacggggggc gtcgccgccc cccgtcgtct
     1261 caggtcactg acaggtcact gtcaggtcag caagggctgc cggcgactag aagtcgtagc
     1321 gtagcgcgag ctggagggtg cgggggttgc cgatccagga gttctcgccg gcgaagccgg
     1381 cggccggagt ctgggcggtg ccccacacgg tgcgggtggt gaaggggttc gtgcggtcgg
     1441 tgcagttgaa gcactcggcg aacagcgtca gctcgccggg acccaggtcg aagcccttgg
     1501 acagccgcat gtcgagcgtc cagaaatcgg gctgacggaa gctgttgcgg tcgaagtgct
     1561 cgccgcccac cgtcggccgg tcggtctcgc ggacgccgtc gttgttgaag tcggagttgg
     1621 tggtggcggt gaagggctgg ccggtcgagt agcggaacga gccggagagc ccgaggcccc
     1681 accacggcgt gtcccacagg ccgttgagca cgcccttcca gcgctggtcg cggttggagt
     1741 agccccagtt gaggtcgagg ttgttgaagt cctcggactg gatgcccgcg aagttgcgct
     1801 cgttggagtc gtgatccttg tcctcggacc aggtgacggc gccgtagagg ctgaagttgt
     1861 cggcgtagcg ccgctgcagg gtggcggtga ccgcggtgta ctccgactcg gcgtcggaga
     1921 cgttcatgat gacgcggtcg tagccggaga gcggccgggt gttggagtac cgcggcaggc
     1981 cgttgggggt cggcgggttg gccggatcgt agacgcggtt gatgtcggtc aggcgctgga
     2041 gctgcttgcc ctcggcatag gtgaagtcga ggccgaagga ggcgagccgg aagatctccc
     2101 gctcgatgcc cagcgtcgcg cggtcggtgt aggggttctc gaagtccgga tctacggtgt
     2161 agacgccggg ggcggcgagg cgggtgaggc ggcccaggtc gatgcgctcg atgccctccg
     2221 ggctccagcc tggtccccag gccggcgacg gcgtgtcggc cggcccgttg gtgatgttgc
     2281 cgttcgcgtc acgccggacg gtgagctggg tcccgcggat gccgttcgag gtgaaggtct
     2341 gcgcccagag gagcgccggc gtccggctcc agtagcgtcc ggcggagaag cggaccgccg
     2401 tcttctcgtc cggcgcccag gagatgccga ggcggggtga gatctggttg tccgagtcgg
     2461 ggatctgcgc ggtcaaccgc agccggccgg cggccgtgtc cacgtcgttc gggttgagga
     2521 tcgcaaagtc cgggttgtcc agccgctccc agcgcacgcc ggccgagatg gtcaggttgg
     2581 gacgcacgaa ccactggtcc tgcaggaaca gcgcggtctc ctcctgtgcg aagtcggacc
     2641 gccccgcctc cgaggaggtg aggccgttca ggccgccgaa ctgccggtac tccgtccagc
     2701 ggccggccag caggtccgcc tcgttgttga agatgaagac gccgcgccag ttgccgcgga
     2761 agacctggtc gacgctggtg tcgttgtact cgaagccggc cttgaggaag tggcggccca
     2821 gcgagtagct cagggtgtcg gcgatcgccc ggcggtcggt cgtcgtctcg atggggagga
     2881 aggccacctc gccgtagcgg gcgctgatcg agcccagggt gacctggatc tccggcaggt
     2941 tgagcccctt gtcgttgcgg ggcatgtcct cggtgatgta gttgatgttg aggtcgttca
     3001 gcaggttggc gccccactgg ctggagtagg aggcgaccca ggagtcgctg ttcagcccct
     3061 cgaggccgtt gaagctcgcc gtccgggtgt tggaggtcga ggtgccgttg atcccctcgt
     3121 actcggtgaa gttgccgcgc agcatgaagc ggtgcgacgg gctcgcctgg aagtcgaggc
     3181 ggccgaagag caccgagccg tcctgagtcg aggaataggt gtcgggcgag gcgagcaccg
     3241 ggtagcggcc gaagatgtcc tggtcgagga ggagcgggtc gatcggcacc gagacgctct
     3301 ggtcctggtt gtcataggag aggaagtaga acagccggtc ctggaggatc gggccgccga
     3361 tcgaggcgcc gtactgctgc ttctcctgct cggccggctc ggtgccgttg gggaagtcgg
     3421 agatcaggtc ctgcggctgg tcgtagtaga aggccgagcc gtgcaggttg ttggtgccgc
     3481 tcttggtgat gacattaacg aagccgccgc ccgagcgccc gaattccacc gaggcgccgt
     3541 tggtgatgac ggtgaactcc ttgatcgact cctgggagat ggagagcggc gcccggccct
     3601 cggccgtgcc caccgtgccg ccgaagaagg cgttgttgaa gtccacgccg tcgatggt
//
LOCUS       DASPJI010000570         7301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_207002, whole genome shotgun sequence.
ACCESSION   DASPJI010000570 DASPJI010000000
VERSION     DASPJI010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207002"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VEL74_15890"
     CDS             <1..141
                     /locus_tag="VEL74_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019480411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HYG64057.1"
                     /translation="DPRSAVVDLPLVQTVGDRLVRVVAWYDNEWGYAHRLADLLEILA
                     TS"
     gene            173..1375
                     /locus_tag="VEL74_15895"
     CDS             173..1375
                     /locus_tag="VEL74_15895"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HYG64058.1"
                     /translation="MPNLITLDDLDLGSLRGKRVFVRVDFNVPLSADHEVLDATRIEE
                     ALPTIRELTGAGARVLLASHAGRPKGERDPRYSLRPVAVKLADLLDQGVRFAPDCVGP
                     EVEKLAAEMDPGEVMLLENLRFHAGETKNAPAFAGQLAALAEVFVDDAFGSAHRAHAS
                     VVGVAERLPEKAAGRLMAREVEALGKLLGEPERPFAALLGGAKIEGKIDTLENLLPRL
                     DILLLGGGMANTFLAAQGHDMAGSLFESDRLDMARDILERARSRGAEVLLPVDVVVAE
                     TLDEPAWVRTVRADQVPAGAKALDVGPETRKAFAKAIGRAKTLFWNGPLGVFEKPPFD
                     EGTNEVARALASFSGYSVIGGGETVAAANRAGVSEQIGHVSTGGGASLEFLAGKTLPG
                     VEILERGR"
     gene            1372..2154
                     /gene="tpiA"
                     /locus_tag="VEL74_15900"
     CDS             1372..2154
                     /gene="tpiA"
                     /locus_tag="VEL74_15900"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HYG64059.1"
                     /translation="MSADPNTRGLIAANWKMHLLRADAERFCQALLAGLPAAGPRVVV
                     FPSFPLIPTVAGALAGSPVGQPVGVGGQDLHPEDKGAHTGDVSGAQLADAGCTWALCG
                     HSERRRDHGEGDELVGSKAAAAVRHGLAPMICVGETRDERRAGRTFEVLERQIRAALA
                     PLAALPAEGRFALAYEPVWAIGTGETATPEIAQEAHRHLRGLLAEIRGEEEAAGLSIL
                     YGGSVTADNAAGLSAQPDIDGFLVGGASLDPEKFLSIIRGSF"
     gene            2194..2541
                     /gene="secG"
                     /locus_tag="VEL74_15905"
     CDS             2194..2541
                     /gene="secG"
                     /locus_tag="VEL74_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HYG64060.1"
                     /translation="MIYLLYTVHVLVCVFLILVVLLQQGKGADLSVFGGGSTQTAFGA
                     RGATTLLHKLTVASFVIFVLTTVGITILQSGPGTSSVMRQVAAEASQPAPAPAPSAPA
                     PAGPAQPANPPAQ"
     gene            2602..2686
                     /locus_tag="VEL74_15910"
     tRNA            2602..2686
                     /locus_tag="VEL74_15910"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2636..2638,aa:Leu,seq:gag)
     gene            3006..3212
                     /locus_tag="VEL74_15915"
     CDS             3006..3212
                     /locus_tag="VEL74_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HYG64061.1"
                     /translation="MAQQGTVKWFNNAKGYGFIQSEVGGEDIFVHHTAIVAEGFRTLN
                     QGEQVNFEIAEGPKGLQARNVTRG"
     gene            complement(3311..3823)
                     /locus_tag="VEL74_15920"
     CDS             complement(3311..3823)
                     /locus_tag="VEL74_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012052536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYG64062.1"
                     /translation="MSTNSESRVTLDTSKGRIVLELDSEKAPQTVENFLRYVKAGHFD
                     GTIFHRVIPGFMIQGGGFTESMSQKPTQASIQNEADNGLKNGRGSIAMARTSDPHSAS
                     AQFFINLKDNDFLNHSGKNAQGWGYAVFGKVVEGMDIVDQIAQVKTGRRGMHDDVPME
                     AVVIQKATAG"
     gene            3822..4445
                     /locus_tag="VEL74_15925"
     CDS             3822..4445
                     /locus_tag="VEL74_15925"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HYG64063.1"
                     /translation="MQASPFRSVADGSTPAGVVLAGGASSRMGLDKALLEESGESLAR
                     RAAGRLAGVCPEVMVADGGRGLVPGLGSVADGPGKGPAAGILGAAAIHPGRPLLVLAC
                     DLPAVPPSLLAEIAASPGDLVLPRWEGRAEPLCALYGPAALAALAGQVAHGRFALHEL
                     AGLEGLAVRWLDEERLTVHGRPAAMFLNLNTPEDLERWRALRALDGV"
     gene            complement(4422..4757)
                     /locus_tag="VEL74_15930"
     CDS             complement(4422..4757)
                     /locus_tag="VEL74_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HYG64064.1"
                     /translation="MVAPALTEVRRLPEEGRMRLTWSDGHSAEYGYDYLRGWCPCAGC
                     QGHTGLKIRYHPPTKPVSAESIKPVGNYAISIAFSDGHGTGIYRYDFLRQICPCPSCG
                     GVSDSVQSS"
     gene            4872..5492
                     /locus_tag="VEL74_15935"
     CDS             4872..5492
                     /locus_tag="VEL74_15935"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HYG64065.1"
                     /translation="MRIMQKTLFILLSLLPASLLAQGPVQRFELTPFVGYRLNTDFEA
                     VSGDFFDADVEIEEGATYGVILDIPLGESGWKLELLANRQESTLIVDEGLLAPTLELG
                     DIDLGIYHAGLLYQWQAGGQVEPFIVFSGGITRLDPDFIGVSAENRLSGSFGGGIKVF
                     FSDNVGLRLEGRGYWTDIDDAELSERWDADDALFQGEASAGLIFAF"
     gene            complement(5494..6993)
                     /locus_tag="VEL74_15940"
     CDS             complement(5494..6993)
                     /locus_tag="VEL74_15940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYG64066.1"
                     /translation="MCLLALQGLETAQATARRVGFVPEDGPDGVRVASVTPGLPGDRA
                     GLRPGDRIVAVGGRRLVQIAGYEEVAEGFRRERPVTFRVERGSRVLDLRVVPGVVPDW
                     GPFGVRAFAVLCYLGIALLSLGQGFEDVRSRLLLGLTSAVALELSLPLEAIGDPGLLA
                     AARAGYDLLTGLQIAFELHLVSLIPDRPAWLRRRPWVVPAYYAAGTAFGAAAGLLCLA
                     EAAGLGTDPGRLEALLVDSGVRLWAVAVCGILAARALWHPELRGRLQAGIVLAGAMPW
                     VLFVLADLLLRSLDVEPPGWLSTLEPLALVCYPAAFLVARHMATLLENARLARSATYE
                     GLTGLLRREAVLERLDRELERALRYGRPLAVAIADLDHFKQVNDRHGHLAGDALLRRT
                     AQVIAGELRGTDAVGRYGGEEFLLVLPETVLEGAWTVAEKIRRRVQEAAVTAEGGAVI
                     RATLSIGLASLEDCRATGGGSARDLIAAADRSLYEAKDGGRNRVWPRAS"
BASE COUNT         1048 a         2581 c         2584 g         1088 t
ORIGIN      
        1 gacccgcgct cggccgtggt cgacctgccg ctggtccaga cggtggggga ccggctcgtc
       61 cgggtcgtgg cctggtacga caacgaatgg gggtacgccc accggctggc ggacctgttg
      121 gagatcctgg caacttcgta gcgtgcaact ggaaggaagg gagcgaattc acatgccgaa
      181 cctcatcacc ctcgacgatc tcgatctcgg gagcctgcgc ggcaagcgcg tcttcgtccg
      241 cgtcgatttc aacgtcccgc tgtcggccga ccacgaggtg ctggacgcca cccgcatcga
      301 ggaggcgctc cccaccatcc gcgagctgac cggcgccggc gcccgcgtcc tcctcgcctc
      361 tcacgccggg cggcccaaag gggagcgcga tccgcgctac agcctgcggc cggtggcggt
      421 caagctcgcc gacctgctgg accagggggt ccgcttcgcc cccgactgcg tcggccccga
      481 ggtcgagaag ctggccgccg agatggaccc gggcgaggtc atgctgctcg agaacctgcg
      541 cttccacgcc ggcgagacca agaacgcccc ggccttcgcc ggccagctcg ccgccctggc
      601 cgaggtcttc gtcgacgacg ccttcggcag cgcccaccgc gcccacgcct cggtggtcgg
      661 ggtggccgag cgtctgccgg agaaggccgc cggccgcctg atggcgcgcg aggtggaggc
      721 gcttgggaag ctgctcggtg agccggagcg ccccttcgcc gccctcctcg gaggggccaa
      781 gatcgagggc aagatcgata ccctggagaa cctgctcccc cggctcgaca tcctgcttct
      841 gggcggcggc atggccaaca ccttcctggc ggcccagggg cacgacatgg ccggctccct
      901 cttcgaatcc gaccggctgg acatggcacg cgacatcctg gagcgggcgc ggagccgcgg
      961 cgccgaggtg ctgctgcccg tcgacgtggt ggtcgccgag acgctggacg aaccggcgtg
     1021 ggtgcgcacc gtgcgcgccg accaggtccc ggcgggcgcc aaggcgctgg acgtcggccc
     1081 cgagacccgc aaggcctttg ccaaggccat cggccgcgcc aagacgctgt tctggaacgg
     1141 tccgctgggt gtcttcgaga agccgccgtt cgacgagggg accaacgagg tggcgcgggc
     1201 cctggcgagc ttctcgggct actcggtgat cggcggcggc gagacggtgg ccgcggcgaa
     1261 ccgcgccggg gtgagcgagc agatcggcca cgtttccacc ggcggcggcg cgtcgctcga
     1321 gttcctcgcc ggcaagaccc tgcccggcgt cgagatcctg gagcgcggcc ggtgagcgcc
     1381 gatccgaata cccgagggct catcgccgcg aactggaaga tgcacctcct gcgcgccgac
     1441 gcggagcggt tctgccaggc cctcctggcg gggctcccgg ccgcggggcc aagggtggtg
     1501 gtcttcccgt cctttcccct gatccccacg gtcgccggcg cgctggccgg cagcccggtg
     1561 gggcagccgg taggggtagg agggcaggac ctccaccccg aggacaaggg ggcgcacacc
     1621 ggcgacgtct ccggcgcgca gctcgccgac gccggctgca cctgggcact ctgcggccac
     1681 agcgagcggc ggcgcgacca tggcgagggg gacgagttgg tggggagcaa ggccgcggcc
     1741 gccgtccgcc acggcctcgc acccatgatc tgcgtcggcg agacgcggga cgagcgccgc
     1801 gccggccgca ccttcgaggt gctggagcgc cagatccggg ccgcgctcgc tcccctggcg
     1861 gccctgccgg cggaggggcg cttcgccctg gcctacgagc cggtctgggc catcggcacg
     1921 ggcgagacgg cgacgccgga gatcgcccag gaggcgcacc ggcacttgcg cggcctcttg
     1981 gcggagatcc gcggcgagga ggaggccgcc gggctgtcca tcctctatgg cgggtccgtg
     2041 acggccgaca acgcggccgg tctgagcgcc cagccggaca tcgacggctt cctggtgggc
     2101 ggggcgagtc ttgacccgga gaaattcctg agtatcattc gtggttcctt ctgacgggcc
     2161 acgaagcgcc cgtccgttcc ggaggtttcg accgtgatct acctgctgta caccgtgcac
     2221 gtcctcgtgt gcgtcttcct catcctggtg gtcctgctgc agcagggcaa gggcgcggac
     2281 ctttcggtct tcggcggcgg cagcacgcag accgccttcg gcgcccgggg cgcgacgacg
     2341 ctcctgcaca agctcacggt ggcctccttc gtgatcttcg tcctcaccac ggtggggatt
     2401 acgatcctgc agtccgggcc gggcaccagc tcggtcatga gacaggtggc ggcggaggct
     2461 tcgcagccgg ctccggcgcc ggctccgtcg gctcctgcac ccgcagggcc ggctcagccg
     2521 gcgaaccctc cagcccagta gggcggcggc cggcccggcc cgagacatcg ggagccgggc
     2581 ctgctacaat gttctttgcg tgccgaagtg gcggaattgg tagacgcgca cgtttgaggg
     2641 gcgtgtgggg caacccgtac cggttcgagt ccggtcttcg gcaccattcc ctcccgttca
     2701 gatcctcccg gatctgattc ccgacctgat caccatctct gctctccttg caggccgtgc
     2761 tttgccctgg ccgcttgcct cggccgttat ctcggccgtt atctcggccg gaaatcactc
     2821 ttgatttcct tcgccgttgg tgacaacatg cgccaaattc tcgtcgaagg gtaagtagtc
     2881 cggctgctgc gcgagtcggt tcgtggtctc acacgagcgg ctctcaacgg cacctcctgc
     2941 gtggatccga gcggccccga gtccgtcgag gggtgcgcgt tttattcaga aaggagtagc
     3001 agcagatggc tcagcaaggt acggtcaagt ggttcaacaa cgccaagggc tatggcttca
     3061 tccagtccga ggtgggtggg gaggacatct tcgtccacca taccgcgatc gtggccgagg
     3121 ggttcaggac gctgaaccag ggcgagcagg tgaacttcga aatcgccgaa ggccccaagg
     3181 gtctgcaggc gcgcaacgtc acgcgcggct gacgcggccc ccctctcgtc cggccgagcg
     3241 ctactccagg gatctgcgat gccgtggaac gccctgccac cggggcgggg cgttcccgct
     3301 tcagccgtcc tcagccggca gtggctttct ggatcaccac cgcttccatc ggcacgtcgt
     3361 cgtgcatccc ccggcggccg gtcttcacct gggcgatctg gtcgacgatg tccatcccct
     3421 cgacgacctt gccgaagacg gcgtagcccc agccctgggc gttcttgccc gagtggttca
     3481 ggaagtcgtt gtccttcagg ttgatgaaga actgggccga ggcgctgtgc gggtcgctgg
     3541 tgcgcgccat ggcgatgctg ccgcggccgt tcttgagccc gttgtcggcc tcgttctgga
     3601 tcgacgcctg ggtcggcttc tgcgacatgc tctcggtgaa gccgcccccc tggatcatga
     3661 agccggggat gacgcggtgg aaaatggtgc cgtcgaagtg gccggccttg acgtagcgga
     3721 ggaagttctc caccgtctgg ggggcctttt cggagtccag ctcgaggacg atccgcccct
     3781 tgctggtgtc cagggtgacg cgagattcag agttcgtgct catgcaggca tctcctttcc
     3841 gttcggtcgc ggatggtagc actccggcag gggtggtgct cgccggcggc gcgtccagcc
     3901 gcatgggtct ggacaaggcg cttctggagg agtccggcga gagcctggcg cggcgggcgg
     3961 ccgggcggct ggccggggtc tgcccggagg tgatggtcgc cgacggcggg cgcggcctcg
     4021 tccccgggct agggtcggtg gcggacggcc ccggaaaggg gccggccgcc ggcatcctcg
     4081 gcgccgccgc catccatccc ggccggccgc tgctcgtcct cgcctgcgac ctgccggcgg
     4141 tgccgccttc cctgctggcg gagatcgccg cctcccccgg agacctggtg ctcccccggt
     4201 gggaaggcag ggccgagccg ctctgcgccc tttacggtcc cgccgccctg gccgccctgg
     4261 ccgggcaggt ggcccacggc cgcttcgccc ttcacgagct tgcgggtctc gaggggctgg
     4321 cggtgcgctg gctggacgag gagcggctca ccgtccatgg gaggccggcg gcgatgttcc
     4381 tcaacctcaa cacgccggag gacctggagc gctggcgtgc cctacgagct ctggacggag
     4441 tctgagacgc cgccgcagct cgggcagggg cagatctggc gcaggaagtc gtagcgatag
     4501 atgccggtgc cgtggccgtc ggagaaggcg atggagatcg cgtagttgcc caccggcttg
     4561 atcgactcgg cgctgaccgg cttggtcggg gggtggtagc ggatcttgag cccggtgtgc
     4621 ccctggcagc cggcgcaggg gcaccagccg cgcaggtaat cgtagccgta ttcggcggag
     4681 tggccgtcgc tccaggtcag gcgcatgcgc ccctcctcgg gcaagcggcg gacctcggtg
     4741 agggcaggcg ccaccatggt tcgttccttt cgtagagacg cgagcacccg gtccgggaag
     4801 ggccggtctc gtgatacaac gcgaaaagcc ggggccggcc ttccggaccg cccggcctcc
     4861 aaggagacat catgcgcatc atgcaaaaga cgctcttcat cctactctct cttttgcccg
     4921 cgagcctcct ggctcaaggg cctgtccagc gcttcgagct cacgccgttc gtgggatacc
     4981 ggttgaacac cgacttcgaa gccgtctccg gcgacttctt cgacgccgac gttgagatcg
     5041 aggagggcgc cacctacggc gtgatcctcg acatccctct cggcgagagc gggtggaagc
     5101 tggagcttct ggccaaccgt caggagagca ccctgatcgt cgacgagggc ctgctcgcgc
     5161 cgaccctcga gctgggggac atcgacctgg ggatctacca cgccgggctg ctctatcagt
     5221 ggcaggccgg cggccaggtg gagccgttca tcgtcttctc cggcggcatc acccggctcg
     5281 atcccgactt catcggggtg tcggcggaga accgcctgtc ggggagcttc ggcggcggca
     5341 tcaaggtctt cttctccgac aatgtcggcc tgaggctgga ggggcgcggc tactggaccg
     5401 acatcgacga cgccgagctc agcgagcgct gggacgccga cgacgccctg ttccagggcg
     5461 aggccagcgc cggcctgatc ttcgccttct gattcaggaa gcccggggcc agacccggtt
     5521 gcgtccgccg tccttggctt catagaggga gcggtcggcc gcggcgatca ggtcccgggc
     5581 gctcccgccg cctgtcgcgc ggcaatcctc taaagacgcg agcccgatgg agagggtggc
     5641 ccggatgacg gcgccaccct ccgccgtcac cgccgcctcc tggacccggc gccggatctt
     5701 ctcggccacg gtccaggcgc cctccaagac ggtctcgggc agcaccagca ggaactcctc
     5761 cccgccatag cgccctacgg cgtcggtgcc gcgcagctcg ccggcgatga cctgggcggt
     5821 ccgccggagc agggcgtccc ccgccaggtg gccatggcgg tcgttgacct gcttgaagtg
     5881 gtcgaggtcg gcgatcgcga cggctagcgg gcggccgtag cgcagggccc gctccagctc
     5941 ccggtcgagc cgctcgagga cggcctcccg gcgcagcagg ccggtgagcc cctcgtaggt
     6001 cgccgaccgg gcgagccgcg cgttctcgag caacgtggcc atgtggcgcg cgaccaggaa
     6061 ggcggccggg tagcagacca gggccagggg ctcgagggtg gagagccagc cgggcggctc
     6121 tacgtccagc gaccggagca gcaggtccgc gagcacgaag aggacccagg gcatggcgcc
     6181 ggccaggacg atcccggcct ggagacggcc gcgcagctcc ggatgccaca gcgcccgcgc
     6241 cgccaggatg ccgcagacgg ccacggccca gagccggacc cccgagtcga cgagcagcgc
     6301 ctccaacctg ccggggtccg tccccaggcc tgcggcctcg gcgaggcaca ggaggccggc
     6361 cgccgcgccg aacgcggtcc cggccgcgta ataggcgggc accacccagg ggcggcggcg
     6421 cagccaggcc ggccggtcgg gaatgagcga cacgaggtgg agctcgaacg cgatctgcag
     6481 gccggtcaac aggtcatacc ccgcccgggc cgccgcgagc agcccgggat cgccgatggc
     6541 ctccaaggga agggagagct ccagggccac cgcggacgtc aggccgagga gcagccggct
     6601 ccgcacgtcc tcgaagccct ggccgaggga gagcagcgcg atccccaggt aacagagcac
     6661 cgcgaacgcc cggacgccaa agggccccca atccggcacg accccgggca ccacccgcag
     6721 gtccagcacc cggctccctc tctccactcg aaaggtgacc ggccgttccc gacggaatcc
     6781 ctccgccacc tcctcgtacc cggcgatctg aaccagtctc cggccgccga ccgcgacgat
     6841 ccggtcccct ggccggagcc cggcccgatc ccccggcagc ccgggagtca ccgaagccac
     6901 ccgcaccccg tccgggccat cctcgggcac gaagccgacc cgccgcgccg tggcctgggc
     6961 ggtctccagc ccttggagag ccagaaggca caccactccc acgacaagaa ttccgcccaa
     7021 ggcgacgcga tcggcacgca ctgaagctct ctgaaccttt ccttcagaga attgtatccc
     7081 gatccggacc cggtcgtctc tcgtgggcgg gggccaagcc ccgcgacgcc tgagacaggc
     7141 acggccgcgc ctgcgattgg cacggcgcgg cccgcgaccg gcacggtgcg gccgatgaca
     7201 ggccgcgcgg cgccggtcag cggcgccacg cggcctgcgg caggcgccgc tcggcctgtc
     7261 accggccggg ctctgcctgt ggcaggcatg gcgacgccac g
//
LOCUS       DASPJI010000571         6860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_783613, whole genome shotgun sequence.
ACCESSION   DASPJI010000571 DASPJI010000000
VERSION     DASPJI010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783613"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1170
                     /locus_tag="VEL74_15945"
     CDS             151..1170
                     /locus_tag="VEL74_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HYG64067.1"
                     /translation="MTDMLDLLVVGAGPTGIAIGAEARREGLDVLVVDRGPLTAAIQG
                     YPTFMEFFTTRERLEIADIPFTIPDAKPTRRQALSYYRGVVERYKIPLAQYEEVLDVR
                     PEDAGDGGFVVRSANPGETEPRRTRRARAVAIATGYFGNPVKLGVPGEDLPWVHSYYR
                     EPYPYYGLDVVVVGAGNTAAEAALELWRAGVRVTLVHRGSGLKTGVKYWLKPDVENRI
                     EEGSIAARFHSVVRAFRGNGGETGGTVEIQTPGGIEELPAQAAFVLVGYTPDAAFERR
                     CGVELDPESLVPVFDPETCESNVPGLYVAGTLQAGRQTNLIFIENSRDHGPRIVQHLK
                     KRLGR"
     gene            1292..2971
                     /locus_tag="VEL74_15950"
     CDS             1292..2971
                     /locus_tag="VEL74_15950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG64068.1"
                     /translation="MRESLIRFVVVGCAFLALAASPAIGQTAGQPKAAEPPPSLFDDV
                     VDVRVVNIEAVVTDKKGERVKGLGPADFSLMVDGKEVPVQYFTEIQDRQAVAPPAGGE
                     VSGLPSLDPGGAVATSYLVFIDEFFSVPVDRDVVLRSLKAQLFRLGPEDSMAVVAFDG
                     RRVSLLSRWTGSQKALAQALDQAIGRNGQGLQRLAELNSFTTLRQTLMDGTGRQERLS
                     LEELDYASSLAEQLERSVAAAANAMRGAASPPGRKVMLLLTGGWPFSVAQYVVRDRMR
                     QPVNRDLPTGEELYGGLVDTANRLGYTIYPVDVKGIQGSGVSARSNSFPVPEDYIREQ
                     EIHTALQHVALETGGRALLDADRAASLERAESDTRSYYWLGFTPEWKGNDQRHDIRLR
                     VLRPGLEVRSRESFLDLSRKAEVGLQVESSMLFGPPPGSPRMMVKLEEPVRRGRSEME
                     LPVTLGIPVAGFTPLQVDGRYVADLVLHVFALDDQGRRSEIPAVPIRVATQEAPSEDR
                     YVPYSTRLLLRRTGQHLVLAVFDPVSGRISTAQIDMAPPGKEEKRGELRLR"
     gene            3055..4764
                     /locus_tag="VEL74_15955"
     CDS             3055..4764
                     /locus_tag="VEL74_15955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG64069.1"
                     /translation="MKNTFSPWLAALACAFLAAAPVASQDQRPAQPPAQEPQPSLFGE
                     QIEVRVVNVEVVATDRQGNRVADLKPSDFRLTVDGKPVQIEYFSEVRGGQAIAPTGGE
                     AAQIAGLPTLAPGEPVGTSYLVFIDDFFTLQTRRDEVLRSLKETLFRLGPEDRMAVVA
                     FDGRGLELLSSWSNSHRNLNRAFDKALSRRAVGAQRIAELRNYRLSRRISVREGFGPM
                     PAFQDRLDDEELDYAVRLRDQVTRSVEAATSSLRGFANPPGRKVMLLLSGGWPFNITS
                     YVVANPARPVYNRYEVPPGEKLIGPLVDTANRLGYTIYPVDVPGIESEGANADVIDVS
                     LQVEDVNNQFPGTTALLREQEVHSALEFVAQETGGRALLNGERVTAFETASNDTRSYY
                     WLGFTPSWQGNDSRHEIRVESLRSDVRVRSRKDYLDLSRKAEVSLMVESAMLFGAPPG
                     TVPMPVQLGKPVPAGRREMDLPISLAIPVAGFQTLPVDGKHVAELELRIFALDGDGGR
                     SDIPVVPIRISTKDLPEAGKHVRYDTKIRLRRTGQHLVVSVFDPLSGKISTAEVDVNP
                     DKG"
     gene            4864..6600
                     /locus_tag="VEL74_15960"
     CDS             4864..6600
                     /locus_tag="VEL74_15960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG64070.1"
                     /translation="MKSHRRVLMACLLAAALTPGLEAQGRAGSRPAQTGPQPQEQAPD
                     DFFIDTVNVSVVNVDVYVTDKKGNRINGLTRDDFELFENGKPMAITNFYAVEEGKAVS
                     APAPEPAPEAPAAGPALPTLDQVETPEDQRLRLVVYIDNFNIAPFNRNRVMRELRVFL
                     NQKLGRDDTVMLVSYDRSLKVRHNFTGDPDLIASALVGLEKHTGAAVHQESDRKQAIR
                     NIQDSQSVGEAKMYARAYAEAAYNDLQFTIGALKETVDALAGLPGRKAVLYVSDGLPM
                     TAGQDVFYAVQNKYGTDGASMMDSFSYDASRRYTELAAQANANRVSFYTIDAAGLRTY
                     SSISAENSAPMEGAVMIDSIQISNMQSPLQLLAEKTGGMAVLNSNVVMPQLERIAQDF
                     NTYYSLGYSPTHFGDGRYHRIEVKVKGRKGLVVRHRDGYRDKPVESRMNDATLATLHF
                     PFEENPLGIDVNFGQVTQREDGLFLVPVNVRIPLGKVTLVPREATNEARVRLFLAAMD
                     SGGDTSEVQQQLVPISIPNADVAAAMDKYYVYSVSLLMRGGEHKVGVGVRDDVAGEAS
                     YLSRGVRVGPRG"
     gene            6617..>6860
                     /locus_tag="VEL74_15965"
     CDS             6617..>6860
                     /locus_tag="VEL74_15965"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HYG64071.1"
                     /translation="MPGGWTDLFRALGESLLQVFKAELAAIEEDFRRTGVHLKASLGL
                     LGVALVLLFWVTGLVIFSLVAILAIWLPVWGAALIVL"
BASE COUNT         1098 a         2332 c         2400 g         1030 t
ORIGIN      
        1 agggtgggag ggcttgccga gggcggtcga gggggccatc ctttatctgc ggggcgccat
       61 ggaagccgcc tatccgttgg gcggcgggca cggccccgtg aatcacctgt accagttgtt
      121 ctgagactgg gaggggttgg agggagagag atgaccgaca tgctcgacct gctcgtggtg
      181 ggggccgggc ccaccgggat cgccatcggc gccgaggcgc ggcgcgaggg gctggacgtc
      241 ctggtggtgg accgcggtcc gctgaccgcc gcgatccagg gctatcccac gttcatggag
      301 ttcttcacca cccgggagcg gctggagatc gccgacatcc cgttcaccat cccggacgcc
      361 aagccgaccc gccgccaggc cctgagctac tatcgcggcg tcgtggagcg ctacaagatc
      421 cccctcgccc agtacgagga ggtgctggac gtccgtccgg aggatgcagg ggacggcggc
      481 ttcgtcgtcc ggtcggccaa cccgggagag acggagccgc ggcggacgcg gcgggcgcgg
      541 gcggtggcga tcgccaccgg ctacttcggc aatcccgtga agctgggcgt cccgggcgag
      601 gacctcccct gggtgcacag ctactaccgg gagccctacc cgtactacgg cctggacgtg
      661 gtcgtcgtcg gcgcgggcaa caccgccgcc gaggccgcgc tcgagctctg gcgcgccggc
      721 gtgcgcgtca ccctggtgca ccgcggctcc gggctcaaga cgggcgtgaa gtactggctc
      781 aagcccgatg tggagaaccg gatcgaggag ggctccatcg ccgcccgctt ccactcggtg
      841 gtgcgggcct tccgcggcaa cggcggagag accgggggaa ccgtggagat ccagacgccg
      901 ggcggcatcg aggagctgcc cgcccaggcc gccttcgtgc tcgtcggcta cacgccggac
      961 gccgccttcg agcggcgctg cggcgtggag ctggatccgg agagcctggt gccggtcttc
     1021 gatccggaga cctgcgagtc caacgtgccc ggcctctacg tcgccggcac cctgcaggcg
     1081 ggccgccaga ccaacctgat cttcatcgag aactccaggg accacggccc gcgcatcgtg
     1141 cagcacctca agaagaggct cgggaggtag gatgaagagt acgccgccgt ccgtattagg
     1201 ggacgaaaga agcaggttct ccccttggat ggcggcggta ttgcccttgc agaaagcgct
     1261 tggaagcatt tctccgaaac gggaggtcac gatgagagag tcgttgattc gcttcgtcgt
     1321 cgttggctgt gcgttcctgg cgctcgcggc atcgcccgcg atagggcaga cggcggggca
     1381 gccgaaggcc gcggagccgc cgcccagtct gttcgacgac gtggtcgacg tgcgcgtcgt
     1441 caacatcgag gcggtggtca cggacaagaa gggtgagcgg gtgaaggggc tcggccccgc
     1501 cgatttcagt ctgatggtgg acggcaagga ggtgccggtc cagtacttca ccgagatcca
     1561 ggaccggcag gcggtcgcac cgcccgcggg cggcgaagtg tcgggcctgc cgagcctcga
     1621 tcccggcggc gcggtggcga ccagctacct ggtcttcatc gatgaattct tttcggtccc
     1681 ggtggaccgc gacgtggtgc tgcgctcgct taaggcacag ctcttccgtc tcggccccga
     1741 ggacagcatg gccgtggtcg ccttcgacgg ccgccgcgtc agcctcctga gccgctggac
     1801 cgggtcgcag aaggccctgg cccaggcgct ggaccaggcg atcgggcgca acgggcaggg
     1861 gctgcaacgg ctggccgagc tgaacagctt cacgaccctg cgccagacgc tgatggacgg
     1921 cacgggccgc caggagcgtc tcagcctgga ggagctcgac tacgccagca gtctggccga
     1981 gcagctcgag cggtcggtcg cggcggcggc gaacgccatg cgcggcgccg ccagcccgcc
     2041 cgggcgcaag gtgatgctgc tgctgacggg agggtggccg ttctccgtcg cccagtacgt
     2101 ggtccgggac cggatgcgcc agcccgtcaa ccgcgacctg cccaccggag aggagctgta
     2161 cgggggcctc gtggacaccg ccaaccggct cgggtacacc atctatcccg tggacgtgaa
     2221 ggggatccag ggctctggcg tgagcgcccg ctccaacagt ttccccgttc cggaggacta
     2281 catccgggag caggaaatcc ataccgctct ccagcacgta gctctggaga cgggtggacg
     2341 ggcgctgctc gacgcggatc gcgccgccag cctcgagcgg gccgaaagcg atacccgctc
     2401 ctactactgg ctgggcttca cgccggagtg gaaggggaac gaccagcggc acgacatccg
     2461 gctccgggtg ctgcgtccgg gcctcgaggt gcggtcgcgg gagagcttcc tcgacctgtc
     2521 gcgcaaggcc gaggtggggc tgcaggtgga gagctcgatg ctcttcggtc cgcccccggg
     2581 atcgccgcgg atgatggtca agctggagga gccggtgcgc aggggacgga gcgagatgga
     2641 gctgccggtg acgctcggca tcccggtggc cggcttcacg ccgctgcagg tggacgggcg
     2701 ctacgtggcc gacctcgtgc tccacgtctt cgccctggac gaccaggggc gccgctcgga
     2761 gatcccggcg gtcccgatcc gggtcgccac ccaggaggcg ccgtcggagg accgctacgt
     2821 cccctacagc acgcggctgc tgctgcggcg gacgggccag cacctggtgc tcgcggtgtt
     2881 cgatccggtg agcggcagga tcagcaccgc ccagatcgac atggcgccgc cggggaagga
     2941 agagaagagg ggagagctgc gattgcggta gcgccccgaa agaaaacgcg gccccggttt
     3001 gttgttgaac aaaagacccc tccggggccg ctctcgtttt tggaggcacc acgaatgaag
     3061 aacacatttt ccccatggct cgccgccctg gcctgcgcct tcctggccgc ggcccccgtg
     3121 gccagccaag accagcgccc ggcccaaccg cccgcccagg agccgcagcc gagcctcttc
     3181 ggtgagcaga tcgaggtccg ggtcgtcaac gtcgaggtgg tggcgaccga ccgccagggc
     3241 aaccgggtcg cggacctgaa gcccagtgat ttccggctga ccgtcgacgg caagccggtg
     3301 cagatcgagt acttttccga ggtccgcggc ggccaggcca tcgccccgac gggtggcgag
     3361 gcggcccaga tcgcgggcct gccgaccctg gcgccgggcg agccggtggg caccagctac
     3421 ctggtgttca tcgacgactt cttcacgctc cagacccggc gcgacgaggt gctgcgctcg
     3481 ctcaaggaga ccctgttccg gctcgggccc gaggaccgga tggcggtcgt cgccttcgac
     3541 ggccgtgggt tggagctgct gagcagctgg tccaactccc atcgtaacct caaccgggct
     3601 ttcgacaagg cattgagccg ccgtgccgtg ggcgcccagc gcatcgccga gctgcgcaac
     3661 taccggctca gccggcggat cagcgtgcgg gagggcttcg gccccatgcc cgccttccag
     3721 gaccgcctcg acgacgagga gctggactat gccgtccggc tgcgcgatca ggtgacgcgg
     3781 tcggtggagg cggcgacgag ctctttgcgc ggcttcgcca acccgcccgg ccgcaaggtg
     3841 atgctgctgc tctccggtgg ctggccgttc aacatcacca gctacgtggt cgccaacccc
     3901 gcccggccgg tctacaaccg ctacgaggtg ccgccggggg agaagctgat cggccccctg
     3961 gtcgacaccg ccaaccggct cggctacacc atctacccgg tggacgtccc cggcatcgag
     4021 agcgagggcg ccaacgccga cgtcatcgac gtgtcgctcc aggtcgagga cgtcaacaac
     4081 cagttccccg gcaccaccgc gctgctgcgc gagcaggagg tccactccgc gctggagttc
     4141 gtggcccagg agaccggcgg ccgggcgctg ctcaacggcg agcgcgtgac cgccttcgag
     4201 accgcctcga acgacacgcg gtcttactac tggctcggct tcacgccgtc ctggcagggg
     4261 aacgactcgc ggcacgagat ccgcgtcgag tcgctgcggt ccgacgtccg ggtgcgctcg
     4321 cgcaaggact acctcgacct gtcgcgcaag gccgaggtgt cgctgatggt ggagagcgcc
     4381 atgctgttcg gcgcccctcc ggggacggtg ccgatgccgg tccagctcgg taagccggtc
     4441 cccgccggcc gccgcgagat ggacctgccg atctccctgg ccatccctgt cgccggcttc
     4501 cagaccctac cggtggacgg caagcacgtc gccgagctgg agctccggat cttcgccctg
     4561 gacggcgacg gcggccggtc ggacatcccg gtggtcccca tccggatctc caccaaggat
     4621 ctgcccgagg ccggcaagca cgtccgctac gacaccaaga tccggctgcg tcggaccggc
     4681 cagcatctcg ttgtgtccgt tttcgaccca ctgagcggca agatcagcac cgccgaggtg
     4741 gatgtcaacc ccgacaaggg atgatgcccg ctcccggcag tccatggggc ctggtcccgg
     4801 ccgtcccgta taatcgggcg ccggatcttc ccgcgcttct gaagagaaag gtacgtgttg
     4861 cgtatgaagt ctcatcgacg agttctgatg gcctgcctgc tggccgcggc cctcaccccg
     4921 ggcttggagg cgcaggggcg cgccggctcc cgtcccgccc agacggggcc ccagccccag
     4981 gagcaggcgc ctgatgactt cttcatcgat acggtcaacg tttccgtcgt caacgtcgac
     5041 gtctacgtga ccgacaagaa gggcaaccgg atcaacgggc tcaccaggga cgacttcgag
     5101 ctcttcgaga acggcaagcc gatggccatc accaacttct acgccgtcga ggagggcaag
     5161 gcggtgagcg cgccggcccc cgagcccgcg cccgaggcgc ccgccgccgg gccggcgctg
     5221 ccgaccctcg accaggtcga gacgcccgag gaccagcggc tgcggctggt ggtctacatc
     5281 gacaacttca acatcgcccc gttcaaccgc aaccgggtga tgcgcgagct gcgggtgttc
     5341 ctcaatcaga agctgggccg cgacgacacg gtgatgctgg tctcctacga ccgctcgctc
     5401 aaggtgcggc acaacttcac cggcgacccg gacctgatcg cctccgccct cgtcgggctg
     5461 gagaagcaca ccggcgccgc cgtgcaccag gagtcggacc gcaagcaggc gatccgcaac
     5521 atccaggact cgcagagcgt cggcgaggcg aagatgtacg cccgcgccta cgccgaagcg
     5581 gcctacaacg acctgcagtt caccatcggc gccctgaagg agaccgtcga cgccctggcg
     5641 ggcctgcccg gccgcaaggc ggtcctctac gtgagcgacg ggttgccgat gacggcgggg
     5701 caggacgtct tctacgccgt gcagaacaag tacggcaccg acggcgccag catgatggac
     5761 tccttctcct acgatgccag ccgccggtac accgagctgg cggcccaggc caacgccaac
     5821 cgggtgagct tctacaccat cgacgcggcg gggctcagga cctactcgtc gatctcggcc
     5881 gagaactccg cccccatgga aggggcggtc atgatcgact ccatccagat ctcgaacatg
     5941 cagagcccgc tgcagctcct ggccgagaaa accggcggca tggcggtgct caactcgaac
     6001 gtggtcatgc cgcagctcga gaggatcgcc caggacttca acacctacta ctcgctcggc
     6061 tactcgccca cccacttcgg ggacggccgg taccaccgga tcgaagtgaa ggtgaagggg
     6121 cgcaaggggc tggtggtgcg gcaccgcgac ggctaccgcg acaagccggt cgagtcgcgg
     6181 atgaacgacg cgacgctcgc caccctccac ttcccgttcg aggagaaccc gctggggatc
     6241 gacgtcaact tcggccaggt gacgcagcgc gaggacggcc tgttcctggt gccggtcaac
     6301 gtcaggatcc ctctggggaa ggtgacgctg gtgccgcggg aggcgaccaa cgaggcgcgg
     6361 gtccggctct tcctggccgc catggactcc ggcggcgaca cctccgaggt ccagcagcag
     6421 ctcgtgccga tcagcatccc caacgcggac gttgcggccg ccatggacaa gtactatgtt
     6481 tactcggtgt cgctgctgat gcgcggcggt gagcacaagg tgggggtggg cgtgcgagac
     6541 gacgtggcgg gggaggcctc ctacctcagc cgcggcgtgc gcgtcggccc caggggctaa
     6601 agggataggg ggaaggatgc cgggtggctg gaccgatctg ttccgtgcgc tgggagagtc
     6661 gctgctccag gtcttcaagg ccgagctggc ggcgatcgag gaggatttcc gccgtaccgg
     6721 cgtgcacctc aaggcgagcc tgggcctcct gggcgtcgcc ctggtgctgc tcttctgggt
     6781 caccggcctg gtgatcttct cgctggtggc gatcctcgcc atctggctgc cggtctgggg
     6841 cgcggcgctc atcgtcctcg
//
LOCUS       DASPJI010000572        12093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_148677, whole genome shotgun sequence.
ACCESSION   DASPJI010000572 DASPJI010000000
VERSION     DASPJI010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12093)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12093
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148677"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VEL74_15970"
     CDS             <1..1201
                     /locus_tag="VEL74_15970"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HYG64072.1"
                     /translation="TDLRRRAASPDAQAPLPMGRARFALSGGWTGQALFFAYLDRALP
                     GRGYDETAVDLLERAFDELGRADAQPGLYAGFTGLAWTVEHLRGWLVDEEDDPAGEIA
                     ERLARLLGARPWPLEYDLISGLVGFGLHALERLPLPPAREAAERAVARLGDLAERGTA
                     GTSWWTEHAVMPEEMRMGEGAGHYNLGVAHGVPGILGWLGQACAAGVETARPLLVTAV
                     PWLLAHQNPPHLASRFSSFFSPGQEWEPSRLAWCYGDLGIAVALMTAARGAGERDWER
                     AALEIARAAAARPMERADVSDPGLCHGAAGNLHLFNRLYQATGEPELAAAARVWLHRL
                     LDMRQPGAGLGGFLVLLPDEHQQRAWRADPGFLTGSAGIGLALLAAATPVEPAWDRLL
                     LASAREA"
     gene            complement(1999..2184)
                     /locus_tag="VEL74_15975"
     CDS             complement(1999..2184)
                     /locus_tag="VEL74_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64073.1"
                     /translation="MKRMLQLAALAISLSLAGQAYTITPVYCGDPCSPNGDRSGCRCG
                     GFRDICTCINGHWSTLY"
     gene            complement(2369..2677)
                     /locus_tag="VEL74_15980"
     CDS             complement(2369..2677)
                     /locus_tag="VEL74_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYG64074.1"
                     /translation="MVSVLESKRESIVAACIRHGVRRLEVFGSAARGELVPGKSDLDL
                     LVEFDPMAPYDRVEAYFDMLEELRSLLDAKVDLVMVDAVKNPYIVADIERTKQTLYTA
                     "
     gene            complement(2708..3118)
                     /locus_tag="VEL74_15985"
     CDS             complement(2708..3118)
                     /locus_tag="VEL74_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYG64075.1"
                     /translation="MGGGAGGGGGALKYLLDSVILIDHFRGHEVASRWLAAHGRESAL
                     SVITRAEVLAGFEARDAAAATGLLDRFPTLGIDRPIADLAAALRREHRWKMPDALQAA
                     LASYHGLTLATRNTRDFSPERHSFVVVPYVLEVK"
     gene            complement(3082..3342)
                     /locus_tag="VEL74_15990"
     CDS             complement(3082..3342)
                     /locus_tag="VEL74_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HYG64076.1"
                     /translation="MIRTLISLDAEEKRWLDRRAEEEGVTMTQIVRTAVRRYREQCEA
                     GEPSLEEMLRRTSGLWKGEEGLAYQDRIRGEWEEGPVAEGER"
     gene            complement(3480..7088)
                     /locus_tag="VEL74_15995"
     CDS             complement(3480..7088)
                     /locus_tag="VEL74_15995"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HYG64077.1"
                     /translation="MTARARTVFGLELRHLFGRPLFWFLVALLFLMSWGMSTGNVSIS
                     SGDASVGGTKAWLTSEFSVAMIVIYMVALVYSFFAAIAAGLTIPADDEAKVGEILHAT
                     PLRPGEYVWGKFLAILAGFLAVLAIHLLFSAFFNHAVPDPERAEIRGPFELVNYLRPA
                     LVFALPALVFILGVSLFLGERWRRPMAVFLFPTALLVLCGFFLWDWAPTWLDPRINRA
                     LMLIDPSGFRWLNETWLKLDRGAGFYNTARIGLDLPFIVSRLAFLGTGLLGVALAQRH
                     LAAHLRGPAGTALPRRLFGRRARPVAAPAWEAEAGARSRSLSALGMSQRPLRFAAATL
                     EVARTELRNLLASPGLYLFTVLILLQAMGNALLALSAFQTKLLLTPGQLAVAAMGPLS
                     AMLSLLLMFYTVESIERDGATGLSAIAYTSPVPTSALLFGKVLANSAVAVLALVATYA
                     GCAIAILAQGEEVGPNPGPFLLVWGLLLLPTFLVWTAFVVAVRAVTGQRFATYGICLA
                     ALVFTGYRAVTGGMSWVGNWPLWANLSWSDMGPFELDRRALLLNRLMFLGLAALLVVL
                     AVRVFNRRQADAIGTIHRLYPRELARQAARLSPWAVVPLVAGAFLYFGVLEGYQGEGM
                     RDRAKDYWTQNLATWKDAPQPDLAGVDLSLDLEPARRWFRTRGSYDLINGRDVRLSRF
                     ALTGGLHWEKVRWSVNGKPYEPEDRSGLWVFTPPGGLPPGGRLRVGFDYEGVFPKGMS
                     ENGGRQNEFILPSGVVLTGFTPSFAPVIGYQEQIGIEEDENDYEPRIYPEDFYHGKTD
                     TLFGFATPFPTRIRISGPEGFTYNSVGTKVSDTVSGGRRTVVWQSDHPVRLFNVVAGR
                     WQERRGRGTVIYHHPGHPYNIDEMSATLDASRRYYSEWFHPFPWKELKLSEFPNLDTY
                     AQGFPTNITFSEGIGFLTRNDEKSDAVFLVTAHEAAHQWWGNILTPGKGPGANILSEG
                     TSHYSTALLMEQVKGPQARMEFLKRLEERYGDERRVDAERPLVLVDGEREADNTLMYD
                     KGGWVFWMLHRHLGRERALAGIRQFFKDWSMGPDYPVLQDFTAALRSQAADPAAYDAF
                     VKQWFHEVVVPEYQLSDARTVKAGDAGGGWRTTLRVKNIGTGRMPVQVAAIRGERFTE
                     DGKPGKGYRDARTTVILGAGEEKRVEILSPFEPEKVLTDPDVEVLQLRRKAALADL"
     gene            complement(7085..8020)
                     /locus_tag="VEL74_16000"
     CDS             complement(7085..8020)
                     /locus_tag="VEL74_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG64078.1"
                     /translation="MLQIRDLVKVYPGPVTALQGIRLDVPPGMFGLLGPNGAGKTTLM
                     KILAGLLEPTSGTVTLDGQDVLEDPQRLWSRLGYLPQDFGFYPHLNGEQMLAFLLKLK
                     GVDAPGGLKRLCAELLERVNLSSAAHRKVKTYSGGMRQRLGIAQAIAGDPRLIIVDEP
                     TAGLDPEERLRFYRLLAELAADRIVLLSTHIVEDVAVLCPRFAVIRKGRLLAQTSPSE
                     ARNAIAGRIFEGRVAADELDDVRRRGLRVTQAVLVEGRHRVRIYEPDGPGGQPPAGFE
                     PVPATLEDAYLLLMQDAEAAIEEPAPAVAAVGGRP"
     gene            complement(8113..8682)
                     /locus_tag="VEL74_16005"
     CDS             complement(8113..8682)
                     /locus_tag="VEL74_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020603069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYG64079.1"
                     /translation="MKLSEARQAFIQAWGRLGAEWGISRTMAQIHALLLTAPEPLDTE
                     AIMEQLHISRGNASTNLRALEDWQLVTREIRPGVRREFFAAEKDVWEMARAIAVQRRR
                     REFDPLMRVLESTTDVEPDQGASAEEVEEFRRMIHGIHEVGSMVSRLFDLVVLLDRST
                     FLEPLLSLLKSRSQPAKLSGGRNLQGGVP"
     gene            8789..9739
                     /locus_tag="VEL74_16010"
     CDS             8789..9739
                     /locus_tag="VEL74_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002611244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HYG64080.1"
                     /translation="MTMVRLHSSFRSLALSCVLAGLAFSPAVAARAESPAAPADTGAA
                     HRQDVESWRKERSDGLRKDDGWLSLVGLFWLDEGENRFGSDPSARVVLPAGKVPANAG
                     TLVRKGNAVTLRVAPGAKVTHQGKPVTEMALDTDAEGKPKVVEMGPVSFFVIQRGDRV
                     GVRVRDKENPALTSFHGMDNFPIDSSWRFEARFEPYQPAKSIPIPNVLGQVSDMPAPG
                     AVVFERDGRIYRLDAIDGGEGKLFLIFADQTSGKETYGAGRFLDTDAPRDGRVVVDFN
                     KSYNPPCALTPYATCPLPPPQNRLALRVEAGEKTFAGGHH"
     gene            10006..>12093
                     /locus_tag="VEL74_16015"
     CDS             10006..>12093
                     /locus_tag="VEL74_16015"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG64081.1"
                     /translation="MATPNVENIYVLSPMQQGLLFHSVYAPGEGLYVDQLVMDLPGDV
                     DAAVLEQAWRHVIARHPVLRTSFHWQGLDKPLQVVHPAVQAPFAVEDWRGAAPERLEA
                     YLEADRRRGFDVTKPPLLRFTLARLSDARSVLLWTYHHILLDAWSGTILNGEVLQVYQ
                     ALAMGRSPELPPVRPFGEYVAWLRRQDPAAVEAFWRDRLAGFRRPTPLALPGLPPEPP
                     LDSGAAGAPDAQGDETRVVPAAASAALRAFARRHQLTLNTVVQGAWALLLHLYGAEDD
                     VVFGATLSGRPAEIPGIESMVGLFINTLPVRVRFAPGDELVPWLRSIQRWQTEMQAFE
                     HSPLAEVQTWSEVPAGRHLFESIVVFENVPGAAAGAAAGAAAGGTAGAPAGQPAGEEL
                     SRYVSRNNYPLSLVVLPEDALRVRALYDRRRFGASAGRMLEHLVALLEAMATAAPGTR
                     VEDLSPLTAAERRRVVEENNATAVPFPAEATVDQLFAAVAAADPGAVALEEPDLRLTY
                     DELNRRANRLAYRLRRLGVGPEEPVGLCVERSADLVVGMLGILKAGGAYVPLDPGYPE
                     ERLNFLLEDTALSVMVTRERELAGLPDGPRRAARPVLLDDAAALARESDADPPPVSSA
                     GGLAYVMYTSGSTGRPKGVEVVHRGITRLVRSGDGFARFGPGEVFLHLAPPSFDAATF
                     EVWGALLNGARLAI"
BASE COUNT         1799 a         4163 c         4325 g         1806 t
ORIGIN      
        1 caccgatctg cggcgccgcg ccgcctctcc cgacgcacag gcgccgctgc ccatggggcg
       61 ggcgcggttc gcgttgtcgg gcggctggac cggccaggcg ctgttctttg cctatctgga
      121 ccgcgccttg ccggggcggg gctacgacga gacggcggtg gacctcctgg agcgcgcctt
      181 cgacgagctc ggccgcgccg acgcccaacc ggggctctac gccggcttca ccggcctcgc
      241 ctggacggtc gagcatctgc gtggctggct ggtggacgaa gaggatgatc cggcggggga
      301 gatcgcggaa cggctcgccc ggctcctcgg agcccgcccc tggccgctgg agtacgacct
      361 catcagcggt ctggtgggct tcggcctcca cgccctggag cggctgcccc tccctccggc
      421 ccgcgaggcc gccgagcgcg ccgtggcccg cctcggcgac ctggcggagc gggggaccgc
      481 cggcacctcc tggtggaccg aacacgcggt catgccggag gagatgcgca tgggagaggg
      541 cgcggggcat tacaacctgg gggtggcgca cggcgtcccg gggatcctcg gctggctcgg
      601 ccaggcgtgc gccgccggtg tggaaaccgc ccgtcctctc ctggtgacag cggtcccctg
      661 gctcctcgcc catcagaacc cgccccacct cgcctcgcgc ttctcctcct tcttctcacc
      721 gggccaggag tgggaaccgt cgcggctggc ctggtgctat ggcgacctgg ggatcgccgt
      781 ggcgctgatg accgcggcac ggggcgcggg agagcgggat tgggagcggg cggccctgga
      841 gatcgcccgc gctgccgcgg cccgtcccat ggagagggcg gacgtgagcg atcccggcct
      901 ctgccacggc gcggcgggca acctccacct cttcaaccgc ctctaccagg ccacgggaga
      961 gccggagctc gccgcggcgg cccgcgtctg gctccaccgg ctcctggaca tgcggcagcc
     1021 gggcgcgggg ctgggcggtt tcctcgtcct cctgccggac gagcaccagc agcgggcctg
     1081 gcgcgccgat cccggcttcc tcaccggctc cgccggcatc ggcctcgccc tgctggccgc
     1141 ggccactccc gtggagcccg cctgggaccg gctgctgctg gcgtcggcac gcgaggctta
     1201 gggaacaagc aggaatccga gcgggcatcc ccccgccgga cgccgcgccc gaatactgta
     1261 agtcaattgt caaagagatg gagccctccg gccctgtaga gggtgaggag gcctccttct
     1321 ggcaggacat cctgccacat gatctacatg acggtctctg ttgactgtgt gatgcctcca
     1381 atgtagtgcg tgccgagccg gtcgggagcg ctttgctgcc gttcctcaat gcttcgctgc
     1441 cattcctcaa cgcttctgct tcatccctca atgttgagga gctgtggcga agtggtccgc
     1501 cgcggcgcca gagcactgag gagccggccc tcaatgctcc gaagtggctc cgtagcggtg
     1561 aggagccgcg gcgaagtgtt gaggaacggc ggcgaagcac tgaggacctg ttcctcaaca
     1621 ctccgccgcc gtccctccgt gtcgaggggc cgcggcgagg cactgaggga tggcccctca
     1681 acacggagga gccgtcccgg aatgctcccg agcggcgccg caccctttag gaacggcgcc
     1741 ggagcactga gggatcgttc ctcaacgttg agggggcgcg gcgaagcact gagggatcgc
     1801 gcctcaggac tgaggagccg tcccggaaag catcccggcg gctctgggtt cattgacaac
     1861 tgaatcgtga cggggcgccg ccgtgtccaa ggccgcgccc ctggggattc ctccgtccgg
     1921 caggagggca gcgctaccgg cagcctgagc tgccggtagc gcctcgccgg ccgaaggcaa
     1981 gctctccgcg agggctatct agtagagggt ggaccaatgt ccgttgatgc aggtgcagat
     2041 gtcgcgaaac cctccacagc ggcacccgga ccggtcgccg ttcggcgagc acggatcgcc
     2101 gcaatagacc ggggtaatcg tgtacgcctg gcccgcgagg gacagggaga tggcgagggc
     2161 ggcgagctga agcattcgtt tcatagagcc tccttggggt gtcgtgaagc aaaactgggg
     2221 tacagcgagt gcgagcatgc attctaggcc gaacggcctt gatcccgccg ccggcccccg
     2281 atattcgaag gcgctcagcc ctcgttcaac actggtagat gtgatcgcgt cacacgcttc
     2341 gatcatgtcg aacagatagg cgcgcgcatc acgcggtata gagagtctgc ttcgtgcgct
     2401 ctatatctgc gacgatgtag ggattcttga cggcatcgac cataacgaga tcgaccttcg
     2461 cgtccagcaa ggacctcagc tcctcaagca tgtcgaaata ggcctcgaca cggtcatagg
     2521 gagccatagg atcgaactcg accagcagat cgaggtcgct cttgcccggg acgagctcgc
     2581 cgcgggcagc cgacccgaag acctcgaggc gccgcacccc atgacgaata cacgccgcca
     2641 cgatcgactc tcgtttgctt tcgaggacgc ttaccattcg acacccctgc aggagtatac
     2701 ggccatctca tttcacctca agcacgtacg gcaccaccac gaacgagtgc cgctcgggcg
     2761 agaagtcgcg ggtgttgcgg gtggcgaggg tgaggccgtg gtagctcgcc agggccgctt
     2821 gtaaggcgtc gggcatcttc cagcggtgct cgcgccgcag ggcggcggcg aggtcggcga
     2881 tgggacggtc gatgcccagg gtggggaagc ggtccaggag gccggtggcc gcggcggcgt
     2941 ccctcgcttc gaagccggcc aggacctcgg cgcgggtgat caccgagagc gccgactcgc
     3001 gtccgtgcgc cgcgagccag cggctggcta cctcatgacc cctgaagtgg tcgatgagga
     3061 tgaccgagtc cagcaggtac ttcagcgctc cccctccgcc accggcccct cctcccactc
     3121 gccgcggatc cggtcctgat aggcgagacc ctcctcgccc ttccacaacc cggaggtgcg
     3181 gcgcagcatc tcctccagcg acggctcccc ggcctcgcac tgctcccgat accggcgtac
     3241 ggcggtcctc acgatctgcg tcatggtcac tccctcctcc tccgctctgc ggtcgagcca
     3301 tctcttctcc tcggcgtcca ggctgatcag ggttcggatc atcgaggggt ccctccggtg
     3361 atatcgctcc gagaaaacga tatcaccggt cgggctcccg tgtccagggt tccgaaggag
     3421 taaagacgga aaacgccgaa aaaatcttgc agaatcttca gccggccgcc tacagccgcc
     3481 tacaggtcgg ccaacgccgc cttgcggcgg agctggagca cctccacgtc ggggtccgta
     3541 aggaccttct cgggctcgaa gggggagagg atctccaccc gtttctcctc gccggcgccc
     3601 aggatgacgg tggttcgggc gtcgcggtac cccttcccgg gcttgccgtc ctcggtgaag
     3661 cgctcgccgc ggatggcggc gacctgcacc ggcatgcggc cggtgccgat gttcttcacc
     3721 cgcagcgtcg tccgccagcc accgccggcg tcacccgcct tcaccgtccg ggcgtcgctc
     3781 aactggtact cggggacgac cacctcgtgg aaccactgct tcacgaaggc gtcgtaggcc
     3841 gccggatcgg cggcctggga gcgcagggcg gcggtgaagt cctggaggac cgggtagtcg
     3901 gggcccatgc tccagtcctt gaagaactgg cggatgcccg ccagcgcccg ctcgcggccg
     3961 aggtgccggt ggagcatcca gaagacccag ccccccttgt cgtacatcag cgtgttgtcc
     4021 gcctcccgct cgccgtcgac gaggacgagc gggcgctcgg cgtcgacacg gcgctcgtcg
     4081 ccgtagcgct cctcgagccg cttgaggaac tccatgcgcg cctgcggccc cttgacctgc
     4141 tccatcagca gggcggtcga gtagtgggag gtcccctcgg acaggatgtt ggcgcctggc
     4201 cccttgcccg gggtgaggat gttgccccac cactggtggg ccgcctcgtg ggcggtcacc
     4261 aggaagacgg cgtcgctctt ctcgtcgttg cgggtgagga agccgatccc ctcggagaag
     4321 gtgatgttgg tggggaagcc ctgggcatag gtgtccaggt tggggaactc ggagagcttc
     4381 agctccttcc aggggaaggg gtggaaccac tccgagtaat agcgccgcga cgcgtccagc
     4441 gtcgcgctca tctcgtcgat gttgtacggg tggccggggt gatggtagat gacggtcccc
     4501 cggccgcgcc gctcctgcca gcggccggcc accacgttga acaggcggac cgggtggtcg
     4561 ctctgccaga ccaccgtgcg ccggccgccg gagaccgtgt cggacacctt ggtccccacc
     4621 gagttgtagg tgaagccctc cggcccgctg atccggatgc gggtggggaa gggggtggcg
     4681 aagccgaaga gggtgtcggt cttgccgtgg tagaagtcct cggggtagat ccggggctcg
     4741 tagtcgttct cgtcctcctc gatgccgatc tgctcctggt agccgatgac cggcgcgaag
     4801 ctgggggtga agccggtgag caccacgccc gagggcagga tgaactcgtt ctgccggccg
     4861 ccgttctcgc tcatcccctt ggggaagacc ccctcgtagt cgaagcccac ccgcaggcgc
     4921 ccgcccggcg gcaggccccc cggcggggtg aagacccaga gcccgctccg gtcctccggc
     4981 tcgtacggct tgccgttcac actccagcgg accttttccc agtggaggcc gccggtgagg
     5041 gcgaagcggg agaggcggac gtcgcggccg ttgatcaggt cgtagctgcc gcgggtgcgg
     5101 aaccagcggc gcgccggctc gaggtccagc gacaggtcga cccccgccag gtccggctgc
     5161 ggggcgtcct tccaggtcgc caggttctgt gtccagtagt ctttggccct gtcgcgcatc
     5221 ccctccccct ggtagccctc gagcacgccg aagtagagga aggccccggc caccagcggc
     5281 accacggccc agggggagag acgcgccgcc tgccgggcga gctcgcgggg gtagaggcgg
     5341 tggatggtgc cgatggcgtc ggcctgccgg cggttgaaga cccgcaccgc caggacgacc
     5401 agcagggccg ccaggcccag gaacatcagc cggttgagga ggagcgcccg gcggtccagc
     5461 tcgaacggcc ccatgtcgct ccacgacagg ttcgcccaga gcggccagtt gcccacccag
     5521 ctcatgccgc cggtcaccgc gcggtagccg gtgaacacca gggcggccag gcagatcccg
     5581 taggtggcga agcgctggcc ggtgaccgcc cgcaccgcca ccacgaaggc ggtccacacc
     5641 aggaaggtgg gcagcaggag cagcccccag acgagcagga acggccccgg gttggggccg
     5701 acctcttccc cctgcgccag gatggcgatg gcgcagccgg cgtaggtggc caccagggcg
     5761 agcaccgcca cggcgctgtt ggccagcacc ttgccgaaga gcagggcgga ggtggggacc
     5821 ggcgatgtgt aggcgatggc ggagaggccg gtggcgccgt cgcgctcgat cgactccacg
     5881 gtgtagaaca tgaggagcag ggagagcatg gcgctcaagg ggcccatggc cgccaccgcg
     5941 agctgccccg gcgtcagcag cagcttggtc tggaaggcgc tcagcgcgag cagggcgttg
     6001 cccatggcct ggagcaggat cagcaccgtg aacaggtaga gccccggcga ggcgagcagg
     6061 ttgcgcagct ccgtccgcgc cacctccagg gtcgccgccg cgaaccgcag ggggcgctgg
     6121 ctcatgccca gggccgacag cgaccgggaa cgagccccgg cctccgcctc ccaggcgggg
     6181 gcggcgacgg gacgggcccg gcggccgaag agccggcgag gcaacgcggt cccggcgggg
     6241 ccgcgcaggt gcgccgccag gtgccgctgg gcgagggcga cgccgagcag gccggtgccc
     6301 aggaaggcga ggcgcgacac gatgaacggc aggtcgagcc cgatgcgcgc ggtgttgtag
     6361 aagcccgcgc cgcggtccag cttgagccag gtctcgttga gccagcggaa gccggagggg
     6421 tcgatgagca tcagcgcccg gttgatccgc gggtcgagcc aggtgggcgc ccaatcccag
     6481 aggaagaagc cacagagcac cagcagcgcg gtggggaaga ggaagaccgc catcggccgc
     6541 cgccagcgct cgcccaggaa gagcgagacg ccgaggatga agaccagggc cggcaaggcg
     6601 aacaccagcg ccgggcgcag gtagttgacc agctcgaacg ggccgcggat ctccgcccgc
     6661 tccgggtcgg gcacggcgtg gttgaagaag gcggagaaga gcagatggat ggcgaggacc
     6721 gccaggaatc ccgcgaggat ggcgaggaac ttgccccaga cgtactcgcc gggccgcagc
     6781 ggcgtggcgt gcaggatctc ccccaccttg gcctcgtcgt cggcggggat ggtgagcccg
     6841 gcggcgatgg cggcgaagaa ggagtagacc agcgccacca tatagatgac gatcatggcg
     6901 acgctgaact cggaggtcag ccaggccttg gtcccgccga cggaagcgtc gccggacgag
     6961 atcgagacgt tgccggtcga catcccccag ctcatcagga agaggagggc gacgaggaac
     7021 cagaacagcg ggcgcccgaa caggtgccgc agctcgaggc cgaagacggt ccgcgcccgg
     7081 gcggtcatgg ccggcctccc acggccgcga cggccggcgc gggctcctcg atggcggcct
     7141 cggcgtcctg catcagcagg aggtaggcgt cctccagggt ggccggaacg ggctcgaagc
     7201 cggcgggcgg ttggcctccg gggccgtccg gttcgtagat ccgcacccga tgccgcccct
     7261 cgacgagcac ggcctgggtg acgcgcagcc cgcgccgccg cacgtcgtcc agctcgtcgg
     7321 cagccacccg cccttcgaag atccggccgg cgatcgcgtt gcgcgcctcg ctgggggagg
     7381 tctgcgccag cagccgcccc ttgcggatga ccgcgaagcg ggggcagagc accgccacgt
     7441 cctcgacgat gtgggtggag aggaggacga tccgatccgc cgccagctcg gcgaggagcc
     7501 ggtagaagcg caggcgctcc tccgggtcga ggccggcggt cggctcgtcg acgatgatga
     7561 gccgcgggtc gccggcgatg gcctgggcga tgccgagccg ctgccgcatg ccgccggagt
     7621 aggtcttgac cttgcggtgg gcggcgctcg acaggttgac ccgctccagc agctcggcgc
     7681 agaggcgctt caggcccccc ggagcgtcga ctcccttgag cttcagcagg aaggcgagca
     7741 tctgctcgcc gttcaggtgg gggtagaagc cgaagtcctg ggggaggtag ccgagccggc
     7801 tccacagccg ctgggggtcc tcgaggacgt cctgaccgtc cagggtgacg gtgccggagg
     7861 tgggctccag caggccggcg aggatcttca tcagggtggt cttgccggcg ccgttggggc
     7921 cgagcaggcc gaacatgccc ggcggcacgt ccaggcggat cccctggagg gccgtcaccg
     7981 ggccgggata gaccttcacg aggtcgcgga tctggagcat ggcgggtcct ttctctctca
     8041 cggaaggcgg tgccttccag cagtgtacgg agaaaagggg ggcgaggttc cagccggtcc
     8101 ggcgggatca ggctacggca cgccgccctg gaggttgcgg ccgccggaga gcttggccgg
     8161 ctggctgcgg ctcttgagca gggagaggag gggctcgagg aaggtgctcc ggtcgaggag
     8221 caccaccagg tcgaacaggc gcgagaccat cgagccgacc tcgtggatgc cgtggatcat
     8281 tcgccggaac tcctccacct cctcggcgga cgccccctgg tccggctcca cgtcggtcgt
     8341 cgactccagc acccgcatca gcgggtcgaa ctcccggcgg cggcgctgga ccgcgatcgc
     8401 ccgggccatc tcccatacgt ccttctccgc cgcgaagaac tcgcgccgca cccccgggcg
     8461 gatctcccgg gtcaccagct gccagtcctc cagcgcccgc aggttggtgc tggcgttgcc
     8521 ccgggagata tggagctgct ccatgatcgc ctcggtgtcc agcggctcgg gagcggtgag
     8581 gagcagagcg tggatctgcg ccatggtccg gctgatcccc cactccgccc ccagccggcc
     8641 ccacgcctgg atgaatgcct gtctcgcctc ggagagtttc atgatggttc cgtagtttcg
     8701 gtcgcgactg aaaatagaga agggaagtgt ccttgtcaag gtggagtaag ctgtcgcctc
     8761 ggcggcgctc tacccgcctg gaggtttgat gaccatggtc cggctccatt cttcgttccg
     8821 ttcgctcgcc ctctcctgcg tgctggcggg tttggcgttt tcaccggcgg ttgctgcccg
     8881 ggcggagtcc ccggccgccc ccgctgacac cggagcggcg caccggcagg acgtggagtc
     8941 ctggcgcaag gagcgaagcg acggcctgcg caaggacgac ggctggctct ccctggtggg
     9001 gctcttctgg ctcgacgagg gggagaaccg cttcggcagc gatccgtcgg ccagggtggt
     9061 gctccccgcg ggcaaggtgc cggcgaacgc ggggacgctg gtgcgcaagg ggaacgccgt
     9121 gaccctccgg gtggcgccgg gggcgaaggt cactcatcaa ggcaagccgg tgaccgagat
     9181 ggccctggac accgatgccg agggcaagcc gaaagtggtg gagatggggc cggtgagctt
     9241 cttcgtcatc cagcgcggcg accgcgtggg ggtgcgggtg cgggacaagg agaacccggc
     9301 gctcacctcg ttccacggca tggacaattt ccccatcgac tcttcctggc ggttcgaggc
     9361 ccgcttcgag ccttaccagc cggcgaagag catcccaatc cccaacgtcc tgggccaggt
     9421 ctccgacatg ccggcgcctg gcgcggtggt cttcgagcgg gacggcagga tctaccgcct
     9481 ggatgccatc gacggggggg agggcaagct gttcctcatc ttcgccgacc agaccagcgg
     9541 caaggagacc tatggcgccg gccgcttcct ggacaccgac gcgccgcgcg acggccgggt
     9601 cgtggtggac ttcaacaagt cctacaaccc gccctgtgcc ctgacgccct acgccacctg
     9661 ccctctgccg ccgcctcaga accgtctggc gttgcgcgtc gaggcggggg agaagacctt
     9721 cgccggcggg caccactgag gcgccggccg ccgctgtccc atttgtcggc agtgccgcac
     9781 cttatctatg ggacggggga aacccccctg cccggccgca gtcgctcgag ctcagcacca
     9841 agagcagatc ccggcgccgg aggaggacgg ccgtcgccgg gaccggtggt tgggttgtaa
     9901 cgaatccctg ctccgcgggc ctgtattccc ggagcgtcgt gctttttgcg atagaacaga
     9961 tcagcgcttt catccgatca tcgccgtacc ctccgcgatc cgcccatggc cactcccaac
    10021 gtcgagaaca tctacgtcct gtcgccgatg cagcaagggc tgttgttcca cagcgtctac
    10081 gcgccggggg aggggctgta cgtcgatcag ctggtgatgg acctgccggg cgacgtcgac
    10141 gccgccgttc tcgaacaggc ctggcggcac gtcatcgccc gccatccggt gctgcgcacc
    10201 tcgttccact ggcaggggct ggacaagccg ctccaggtgg tgcatccggc ggtgcaggca
    10261 ccgttcgcgg tcgaggactg gcggggcgcg gcgccggaac gtctggaggc ttacctggag
    10321 gcggaccggc ggcgcggctt cgacgtcacc aagccgccgc tcctgcgttt caccctggca
    10381 cgcctctccg acgcgcggag cgtgctcctc tggacctacc accacatcct gctcgacgcc
    10441 tggtcgggca ccatcctcaa cggcgaggtg ctgcaggtct accaggcgct ggccatgggg
    10501 cggtctccgg agctgccgcc ggtccgccct ttcggcgagt acgtcgcctg gctgcgccgg
    10561 caggacccgg ctgccgtcga ggctttctgg cgtgaccggc tcgccggctt ccgtcgcccc
    10621 acgccgctcg ccctgccggg cctgccgccg gagccgccgc tggatagcgg cgcggccggc
    10681 gcgccggacg cccaggggga cgagacgcgg gtggtgcctg cggcggccag cgcggcgctg
    10741 cgcgccttcg cgcgccgcca tcagctcacg ctcaacaccg tggtccaggg ggcctgggca
    10801 ctccttctcc atctctacgg tgccgaggac gacgtagtct tcggcgccac cctctcggga
    10861 cgcccggcgg agatcccagg catcgagtcg atggtggggc tgttcatcaa taccctgccg
    10921 gtccgggtcc gcttcgcgcc gggcgacgag ctggtgccct ggctgcgctc gatccagcgc
    10981 tggcagacgg agatgcaggc tttcgagcac agcccgttgg ccgaggtcca gacgtggagc
    11041 gaggtgccgg ccggccggca cctcttcgag tccatcgtgg tgttcgagaa cgtccccggc
    11101 gccgctgccg gcgccgctgc cggcgccgct gccggcggca ccgccggagc gcctgccggc
    11161 cagcccgccg gtgaggagct gtcgcgctac gtatcgcgca acaactaccc gctgagcctg
    11221 gtggtgctgc cggaggacgc gctacgggtg cgggcgctct acgaccgccg ccgcttcggc
    11281 gccagcgccg gccggatgct cgagcatctg gtcgcgctgc tcgaggcgat ggccaccgcc
    11341 gctcccggca cgcgggtcga ggacctgtcg ccgctgaccg ccgccgagcg ccggcgagtg
    11401 gtggaggaga acaacgccac cgccgttccg ttcccggcgg aggccacggt cgaccagctc
    11461 ttcgcggccg tcgccgccgc tgatcccggc gccgtggcgc tcgaggagcc ggacctgcgg
    11521 ctcacctatg acgagctgaa ccggcgcgcc aaccggctcg cctaccggct gcgccggctg
    11581 ggcgtcggtc ccgaggagcc ggtgggcctc tgtgtcgagc gctcggccga tctggtggtc
    11641 ggcatgctcg gcatcctcaa ggccggcggc gcctacgtgc cgctcgaccc cggctatccc
    11701 gaggagcggc tgaacttcct gctggaggac acggcgctct cggtgatggt gacccgggag
    11761 cgcgagctcg ccggtctgcc ggacgggccg cgccgggcgg cgcggcccgt gctgctcgac
    11821 gacgcggcgg cgctggcgcg cgagagcgac gccgatccac cacccgtcag cagcgccggc
    11881 gggctcgcct acgtgatgta cacctcgggc tccaccggcc gccccaaggg ggtcgaggtg
    11941 gtgcaccggg ggatcacccg gctggttcgc tcgggcgacg gcttcgcccg cttcggccct
    12001 ggcgaggtgt tcctgcacct cgcgccgccg tcgttcgacg ccgccacctt cgaggtctgg
    12061 ggggccctcc tcaacggcgc ccgtctggcg atc
//
LOCUS       DASPJI010000573         9839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_207175, whole genome shotgun sequence.
ACCESSION   DASPJI010000573 DASPJI010000000
VERSION     DASPJI010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9839)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9839
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207175"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..526)
                     /locus_tag="VEL74_16020"
     CDS             complement(323..526)
                     /locus_tag="VEL74_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64082.1"
                     /translation="MRVPVDLNDLLAAYEWVSAGETAAVDAKAYISRVTGEVHWIGEG
                     IDEEPPEDIDDGSRYMQCRIRTN"
     gene            2465..3022
                     /locus_tag="VEL74_16025"
     CDS             2465..3022
                     /locus_tag="VEL74_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64083.1"
                     /translation="MSSFTEGTTVSAQSLPNAFNTAFLGRLAERDEPPTAGEADVAGP
                     WRIEPIPNHGFGLFRMGESLTRGFAPTAVFPSRWLALLMAGILPGTGKDPLLLLSRQP
                     NSDGVYPVALAGGGDGGDGGDEPPGSRGLVGFFQLFDEKALDALNVVIHLIQAPEALA
                     YVLEAAGSVTLERCGAILDERAATE"
     gene            3290..3526
                     /locus_tag="VEL74_16030"
     CDS             3290..3526
                     /locus_tag="VEL74_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64084.1"
                     /translation="MSVQITIRDVPDAVRDELAARAALQGKSMQEYLRAELERLAARP
                     PVEKVLERIRERKATAGTRLSPEEILGHRDADRR"
     gene            3523..3924
                     /locus_tag="VEL74_16035"
     CDS             3523..3924
                     /locus_tag="VEL74_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG64085.1"
                     /translation="MSAVVDSSLLVAATSDSGAEGRWAEDVVRAGGLVTPHLALVEAT
                     NILRRFELEGRLGRMEAAAAARDLLLFNLELVPFAPFAERVWELRTNVTSYDAWYVAV
                     AEQFDLPLATLDRRLARSTGPRCRFLLPPLA"
     gene            complement(4526..6673)
                     /locus_tag="VEL74_16040"
     CDS             complement(4526..6673)
                     /locus_tag="VEL74_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64086.1"
                     /translation="MKSRPVEPRRVLAATVLVLLAAAIAWACGPFFPAWILGTDHRVL
                     EAPVLRFADELPRLLPKDGPRLQAVPADGPLYHQTATVDREELAKALEAAGVPAARRT
                     ELLAAHQRLREAFSTMPPPYVEGMSDEEPPPPEEPDSDLSIPAGLPGEFEDYLRGAVL
                     FYQRGPDEARAQWRRLLERPESERRYRSTWAAFMLGKLALREDPADAVRWFERTRELA
                     SQGFADGLGLAASSLGWQARAEWDLGRPERAFQLYAQQAAAGDPTAPASLRFTSSRAL
                     EAGGDVLGRVARDPEARRIVTAYVITPRWRESWGDPTPIYPDARTWLEAVQAAGVTDV
                     DGADRLAWAAYMGGDFAAAGQWAERSPDAPLARWIRAKLLLRDGKLREAEEILAGVAR
                     DLPAANLSPEDAFYWAYDVKMPLATGPRAAGEAGAIALSRGDYVAALDRLLRSGYWTD
                     AAYVAERVLTVDELRAYVGERWPAEPAAPGDAREREFFDEPFGGVLAPDPDETGRSLR
                     YLLARRLARLGHFDAARPYLPEEMRAPLDELTAALDRGRDSRRPRAERAEALFRAACI
                     TRHQGMELQGTEVEPDWTLFGGSYDAAFMTEERMETRKSNVRLPPAEDELRRAARHLA
                     KPGKRFHYRYDAADLAREAAALLPDGSEEKARMLAVGGNWIKLRDPQAALPLYRELTR
                     CCRRTDLGREAARRHWFPEVEEPAGCAPVVENQ"
     gene            complement(6654..7907)
                     /locus_tag="VEL74_16045"
     CDS             complement(6654..7907)
                     /locus_tag="VEL74_16045"
                     /inference="COORDINATES: protein motif:HMM:NF022784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3142 domain-containing protein"
                     /protein_id="HYG64087.1"
                     /translation="MRALALLAVALGLACTAPPISGPLPHEAYLWQRQWTPAVREAVG
                     ESNLAGLVVLAAEVDLSRLPPRVSRAGYDAAALRGTGRPVGLAIRIGSFSRGGGRFRA
                     EPGQIGFLAGLAREVVSEARERGIAPAELQLDYDCPESRLDEYPALVRAVREAVAPVP
                     VVLTALPTWLRHERALRDLLDAADGWVLQVHSFEPPAGPDAPIVLCDPVAARRWVEEA
                     ARLGRPFRVALPTYGYVAAFDARGKLLGLSAEGPALSWPAGVRLRTARSDPAAMAGLV
                     REWTRERPRELAGILWYRLPSAGDRLNWARPTFQAVLAGRTPRSGLRAERRAPEPGLV
                     EIELRNDGEADAPWPPSLRIRWSAGELLAADGLAGYRMRRHGPLEIDLERGPSGDPLR
                     PGERRTVAWLRFASPTEVSLEVSPR"
     gene            complement(8494..9183)
                     /locus_tag="VEL74_16050"
     CDS             complement(8494..9183)
                     /locus_tag="VEL74_16050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG64088.1"
                     /translation="MIRFHQVSFSYGPGEPVLSEVDVHIGPGLTLLVGPNGCGKSTLL
                     KMAAGVERPDSGSVEVSGHDLWTQEVAARRSLAYLSEQPDLTPYATVREILELVRRLR
                     GEPRERVQEALDFVKLGTLAHRSVRQLSMGQRRKAVLAAALIGTPRHVLLDEPLETMD
                     RGARDDILAWIDRLLAAGAAVLVVSHDLEPFTPRAARALTVRDGRCLVVDPLPEEGRA
                     ELLERLARGEV"
     gene            complement(9186..>9839)
                     /locus_tag="VEL74_16055"
     CDS             complement(9186..>9839)
                     /locus_tag="VEL74_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64089.1"
                     /translation="PLPGGGSPSLDLALFSARIAWRALGWKLVSAYFGALLPLGAAWA
                     FIANNELAPKHVILGSLLGGGCAVVLLFAQLGEALAAFRPAWPWSRSLPWSATGRVIA
                     DGAFLALHALPLLILAVLLHPMALVLFGLAPFLALRAAGAMRRAPERRTGASGEILLE
                     GLPAVAATALLPWVVLPLLAMTPLAVRWAAERERRQKVSRWLELHHLAVGDSQSWSA"
BASE COUNT         1634 a         3439 c         3279 g         1487 t
ORIGIN      
        1 agggagcggg ggaggaccga ggaggggggt tgcttttggg gggagggcgg cggcttcgcc
       61 agcattccag cgcaggggca gctagttagc cgccgctggc tttgctggac acgatgaagc
      121 cattttcggc gcaccatccc ctcagagctt cttccttcgc tgcttgctcg taccgatacc
      181 aagcgtcgag ttgcccagcc tgggcgagca agtccttgaa tcgctggtat acacaccgcg
      241 tctgcggaag aaatctcgga ctgtgctgcc tgcacgagga agttgctcgt cgacgaagcg
      301 aaacaccaag gaacggccga gatcagttcg ttcttatgcg gcactgcatg tacctgctcc
      361 catcgtcgat atcctcgggc ggctcctcgt cgatgccctc tccgatccag tggacctctc
      421 ccgtcaccct gctgatgtat gccttggcat cgactgccgc ggtctcgcct gcactcaccc
      481 attcgtaggc agcgagtagg tcgttcagat cgacagggac cctcatgttc gggttcggcg
      541 gccaacgtgc tgcgcttcac cggcggacgg cgaagccggc cgtccgcgtg caagcgccgg
      601 gttcgccggt ctccgcccta tatctacctt agcgcgttaa gcgtcaccgg ggctatgtat
      661 tttccgctgg gatcgcgtac tccctggagt acaaagccaa acgcgtttaa tatcctacct
      721 agagtcacct ccagaaggga agcaaacata ctaggctcct cggcaacaac agcatgcgaa
      781 acttctcgaa cttccaaagg tcgccctgaa acactatccc aatcattaga gactacgagg
      841 caaagcttag gtaagccgat tactgcaaga gtaagcacca tgttcttaaa aggaagtctt
      901 agctcagacc ataaacgggc aagagatgcg acttgaaact cagcgaagcg tacatattca
      961 aagtaaggca gtaagtgctc gcatgtatcc agtcgatgac tacgcccccc aaaaagtagc
     1021 gtccgactgt tggaccgaat ttgggcaccc aatttgataa gcgcatctag gcatatagcg
     1081 atctcagcgt gccccgtgcg aaacacctct atgcgataac ctctgccatc ggcaagaagt
     1141 tcgaaggcct cagcaaccca accattagct gatgtctcga tatggtagcg atttggccta
     1201 gagctaacag agcaccattc gggtagctca agactctcaa tacagtgtct gatttccaag
     1261 ctttcagcct gtatagctgc atcatcaagt tctaacggag taagcgaagc ataggaccag
     1321 gtctgactcc tctgtgacca gtaggtgatc cggtcattcc ggaatggttc agctcgctgt
     1381 aacggtagaa ctagatgccc tcgatcgatc tgctcctgaa tccattgttc atgaagcgcc
     1441 ttaatctcat gaagcctttc gacaggccac agcgcttctt ggcgatctac ctcggtatga
     1501 tggttcggac agaggaggac taaattgccg tagtcatcac gctcttgggc gcttagtgag
     1561 gaacttcctc tcggaccgtt ggccgatctc gcgacgatat gggcaatctc tccgagggtt
     1621 ccttctagtt caaggagggc tagaggaatt cggcagatac tgcaccgccc tcccgctctc
     1681 ccccagagcc tgtaagcatc agttggccta attgccaatc ttagtccctc cgttgtgggg
     1741 ctggtttatc ctgtgccggc gaaccgcttg cctgagctta tgcgacccgt cgtataagcc
     1801 tccgtaacaa cagcccgccc ttccccctat acctttgacg cggtgcaact tacgaaactc
     1861 tacgcctcac cctggagggc tgtcaatgcg tccggcgagg attccatggg aagccctgat
     1921 cgatacatgc aacttcctgc atgcagccgc ctatacgtcc aagaggccct gctgcgagag
     1981 ggatccaaag ctctgacgcg aagagttcga atgtcctgac aggtggggct cggggggcga
     2041 tggtgccgga taagcttcca aaatcacctc ccttgatctt tcttatccca cctccagtga
     2101 cgtataggcg gcggagggca gagggggcac ggccgcttat ctgtccgagc ggtgtcgcat
     2161 tggcggcggc ggccatggcg tcacccctcg ggcgagccac gcctgccctg aggcggcgga
     2221 aggagagcgg gcgcagaaac agcgaagatc cctcttgacg ccttatctcc acctggtcta
     2281 tgctaccgac actcgacgct cccggagaca ctccaagatc cacggatcgc ggaacctgga
     2341 gcggttcgag ggaatctggc tgtagaagcg ctgcaaaggg cggtcggcaa tcctgctgac
     2401 ggcccggagg tgagttcgca tctcaccccc gagcctgacc gcccacagtc cgaaaggagg
     2461 actcgtgagc agcttcacag aaggtaccac tgtctccgcc caatccctcc ccaacgcgtt
     2521 caacaccgct ttcctcggcc gtctggccga gcgggacgag ccgcccacgg ccggcgaggc
     2581 cgatgtggcc gggccctggc gcatcgaacc cattccgaat cacggcttcg gcctgttccg
     2641 gatgggcgag agcctgaccc gtggcttcgc tccgacggcg gtcttcccct cccgctggct
     2701 ggcactcctc atggcgggca tcctccccgg tacggggaag gacccgctgc tcctcctctc
     2761 caggcagccc aactccgacg gcgtctatcc cgtcgccctc gccggcggcg gggatggcgg
     2821 cgacgggggc gacgagcctc ccggctctcg cgggctggtc ggcttctttc agctcttcga
     2881 tgagaaggct ctggacgcac tgaacgtggt catccacctg atccaggcgc cggaggctct
     2941 ggcctatgtg ctggaggcgg ccggatcggt gaccctcgag cggtgcgggg cgatcctcga
     3001 cgagcgggcc gcgaccgagt agggacacat ccttatctat agggggcgcc atctcgacgg
     3061 tggcgccctc cttccaccct ccccaaaaag aagctgcccc cctcctgacc tcccccactc
     3121 cctaccgccc ctccacccag ggggggaggc accacctccc ctgccgcccg gagttggtgt
     3181 ggaccgcctc cccctctccc gggggggccg aggagtgagg gcccccggcg cctcagaact
     3241 ggcctaccag gtgcttgcac tttgcagtca gcagcgtcat gatgcaggca tgagtgtaca
     3301 gatcacgatc cgtgacgtgc cggacgcggt gagggatgag cttgccgcgc gggcagccct
     3361 ccagggcaaa tcgatgcagg aatatctgcg cgccgagttg gagcgtctgg ccgcccgccc
     3421 gccggtcgag aaggtgctcg agcggatccg ggagcgcaag gcgacggcag gaaccaggct
     3481 ctccccggag gagatcctgg gccaccggga cgcggaccgc cggtgagcgc agtcgtcgac
     3541 tcgtctctcc tggtcgccgc tacgtcggat tcgggcgcgg aaggaaggtg ggccgaggac
     3601 gtggtgcgcg ccggcggcct ggtaacgccc cacctggctc tcgtcgaggc cacgaacatc
     3661 ctccgccggt tcgagctcga gggccggctt ggccggatgg aagccgccgc tgctgcccgt
     3721 gaccttctcc tattcaacct cgagctggtt ccctttgccc cgttcgcgga gcgggtctgg
     3781 gagttgcgga ccaacgtcac gagctacgac gcctggtacg ttgcggtggc cgaacagttc
     3841 gatctcccac tggcgacact cgatcgccgc ctggccagat ccaccggccc tcgctgccga
     3901 ttccttctgc cgcccttggc ctgaggtgga ggacgggcgg gaaccccggg cggagcctga
     3961 gggatcattc ctcaatactc cgccgcgatc cctcaagact gaggggccac tccggagcgt
     4021 tgagggatca tccctcaagg ctccgccgcc gctcctcaat attgagggat gacgccggag
     4081 cgttgaggag cggtccctca gtgctccgcc gccgcgcctc aacactgagg gacggctcct
     4141 caacagtccg ctaccgttcc tcaagactga gggatgactc cggagcattg aggagcgatc
     4201 cctcaaagct ccgctgccgc gcctcaccat tgagggacga ctccggagtc ttgagggatg
     4261 atccctcaac agtccgccgc cgtccctcaa gactgaggga tgacggcgga gctttgagga
     4321 gcggtccctc agtgctccgc cgtcgcgcct caacactgag ggatgacgcc ggagtcttga
     4381 ggaacggccc ctcaaggctc ggcgccggcg cggactttag cggagggaaa aaaaaccctc
     4441 ctgacctccc ccgctcccta ccgcccaccc ttccaggggg gaggcaccaa cctccccttg
     4501 cagcctaaag gtagcgtcta ccccgtcatt ggttctcaac caccggcgcg cagcccgccg
     4561 gctcttccac ctccgggaac caatgacgcc gcgccgcctc gcgtcccagg tccgtccgcc
     4621 ggcagcagcg ggtcagctcc cggtagagcg gcagggcggc ctgcgggtcc cgaagcttga
     4681 tccagttgcc ccccaccgcc agcatccgcg ccttctcctc cgagccgtcg ggcaggagcg
     4741 ccgccgcctc acgggccagg tcggcggcgt cgtaccggta gtgaaagcgc ttgccgggct
     4801 tcgccagatg gcgggcggcc cggcgcagct cgtcctcggc cggagggagc cggacgttgc
     4861 tcttccgggt ctccatccgc tcttcggtca tgaaggcggc atcgtaggag ccgccgaaga
     4921 gggtccagtc cggctccacc tcggtcccct ggagctccat cccctgatgg cgcgtgatgc
     4981 aggcggcgcg gaacagggcc tcggcccgct cggcccgcgg ccggcggctg tcccggcccc
     5041 ggtcgagggc ggcggtcagc tcgtcgagcg gcgcccgcat ctcttcaggt agatagggcc
     5101 gcgccgcgtc gaaatggccg aggcgggcga ggcggcgggc gaggaggtag cgcaggctcc
     5161 gcccggtctc gtccgggtcc ggcgcgagga ctcccccgaa cggctcgtcg aagaattccc
     5221 gttcccgagc gtcgccggga gcggccggct ccgccggcca gcgctcgccg acgtaggcgc
     5281 gcagctcgtc cacggtcagc acccgctcgg ccacgtaggc ggcgtcggtc cagtagccgc
     5341 tgcgcagcag ccggtccagg gcggcgacgt agtccccccg ggagagggcg atggcccccg
     5401 cctcccccgc cgcgcgcggt ccggtggcga gcggcatctt gacgtcgtac gcccaataga
     5461 aggcgtcctc cgggctgagg ttcgccgcgg gcaggtcgcg ggcgacgccg gcgaggatct
     5521 cctcggcctc ccgcagcttc ccgtcgcgca gcagcagctt ggcccgtatc cagcgggcca
     5581 aaggcgcgtc aggagaccgc tccgcccact gcccggccgc cgcgaagtcg ccccccatgt
     5641 aggccgccca ggcgaggcgg tcggcgccgt ccacgtcggt gaccccggcg gcctggacgg
     5701 cctcgagcca ggtccgggcg tccgggtaga tgggcgtcgg gtcgccccag gactcgcgcc
     5761 agcgcggggt gatcacgtac gcggtgacga tccgccgcgc ctcgggatca cgggcgaccc
     5821 ggccgaggac atccccgccg gcctccaggg cgcgggagct ggtgaagcgg agcgaggccg
     5881 gcgccgtggg atcgcccgcg gcggcctgtt gggcgtagag ctggaaggcc cgctcgggac
     5941 ggccgaggtc ccactccgcc cgcgcctgcc agccgaggct cgaggccgcg aggcccaggc
     6001 catcggcaaa cccctgagaa gccagctcgc gggtgcgctc gaaccagcgc acggcgtccg
     6061 cggggtcctc ccgcagggcg agcttgccca gcatgaacgc cgcccaggtg gaccggtagc
     6121 ggcgctcgct ctcgggcctc tccaggagcc gcctccactg ggcgcgggcc tcgtcgggcc
     6181 ccctctggta gaaaaggacg gcgccgcgca ggtagtcctc gaactccccc ggcaggcccg
     6241 cggggatgga gagatcggaa tcgggctcct cgggcggcgg cggctcctcg tcactcatcc
     6301 cctccacgta cgggggcggc atggtggaaa acgcctcccg cagccgctgg tgcgctgcca
     6361 gcagctccgt ccgccgcgcc gcgggaacgc cggccgcctc cagcgccttc gccagctcct
     6421 cccggtccac ggtggcggtc tggtgataca ggggaccgtc cgccggcacc gcctggagac
     6481 ggggtccgtc cttcggcagc agccgcggaa gctcgtcagc gaagcggagg accggggcct
     6541 cgagcacccg gtggtcggtc cccaggatcc aggccgggaa gaaggggccg caagcccagg
     6601 cgatggcggc ggcgagcagg accagcacgg tggccgcgag gacgcggcgc ggctcaacgg
     6661 ggcgagactt caagggaaac ctccgtgggg gacgcgaagc gcagccaagc gaccgtccgg
     6721 cgctcgccgg ggcggagagg atcgccggaa ggtccgcgct cgaggtcgat ctccagcggg
     6781 ccgtgccggc gcatccggta gccggcgagg ccgtcggcgg cgaggagctc cccggcgctc
     6841 catcgtatcc gaagggaggg gggccacggc gcgtccgcct cgccgtcgtt gcgcagctcg
     6901 atctccacca gccccggctc gggagcccgc cgctccgccc ggagaccgga ccgcggcgtc
     6961 cgaccggcga gcacggcctg gaaggtgggg cgcgcccagt tgaggcggtc ccccgcgctc
     7021 ggcaggcgat accagaggat gcccgccagc tcgcgcgggc gttcccgggt ccactcgcgc
     7081 accagcccgg ccatcgccgc cggatcgctc cgcgccgtcc gcagccgcac cccggccggc
     7141 caggagaggg cgggcccttc ggccgagagg cccagaagct tcccgcgggc gtcgaacgcg
     7201 gcgacatatc cataggtagg gagcgccacc cggaacggcc ggccgagccg cgccgcctcc
     7261 tccacccaac gccgcgccgc caccggatcg cacaggacga tgggcgcgtc cggcccggcc
     7321 ggcggctcga aggagtgcac ctggaggacc cagccgtccg ccgcgtcgag caggtcccgg
     7381 agcgcacgct cgtgccgcag ccaggtgggg agcgcggtga ggacgaccgg caccggcgcc
     7441 acggcctcgc gcacggcgcg gacgagggcg gggtactcgt ccagccgcga ctccgggcag
     7501 tcgtagtcga gctgcagctc cgccggcgcg atcccccgct cccgcgcctc gctcaccacc
     7561 tcgcgggcga gcccggcgag gaatccgatc tgccccggct cggcccggaa gcgcccgcca
     7621 ccgcgggaga aggagccgat ccggatcgcc aggcccaccg gccggcccgt gccgcggagc
     7681 gccgccgcgt catatcccgc ccggctcacc cggggaggca gccgcgagag gtccacctcg
     7741 gcggcgagga cgacgagccc ggcgagattg gactccccca ccgcctcgcg taccgccgga
     7801 gtccactggc gctgccagag gtaggcctcg tgcggcaggg ggccggagat cgggggggcg
     7861 gtgcaagcga gacccagagc gacggcgagg agagccaggg ccctcacccc ccgccgtcgt
     7921 cgcccgccac cgcccccgga aacgggggcg gcgccatttt ggcccgtttt ggagccgtcc
     7981 ccagctagct gggggcctct ccaccctgag tgggagaggt cccaccctga agcggagagc
     8041 tcccaccctg gagcgggcat gtcccaccct ggaatggaga ctccccaccc tggaccggag
     8101 aggtcccatc ctgaagggga gactctccca ctcagagtgg aggctctccc ctccagggtg
     8161 gagccccccc accctggggt ggagactctc cagtctgaaa atggagcctc ccaccctgga
     8221 ccgggaaggc tccggaatct gtagcaacgg ctccacgctg aatgggaacg gctcctgtcc
     8281 aggaatggac ggctcctgtc tgaatgggag aggcttcccg ctgagcgcga gccgttccgt
     8341 tccagggtgg gagggttcat ttccaggtgc gaacggtttc tagctgaatg ggaacggcgt
     8401 ctggctgaat ggggatggct ccggtccgaa ggcggatggc tccgggctgg ggggaggcat
     8461 caaccctctg cagccatgac tccgtgttgg tccctagacc tccccccgcg ccaaccgctc
     8521 cagcagctcc gcccgcccct cctccggcag cggatcgacc accaggcagc ggccgtcgcg
     8581 gaccgtgagg gcgcgggcgg cgcggggggt gaagggctcg aggtcgtggg agacgaccaa
     8641 cacggcggcg cccgcggcga gcagacggtc gatccaggcg aggatgtcgt ccctcgcgcc
     8701 gcggtccatg gtctccagcg gctcgtcgag gaggacgtga cggggggtgc cgatgagcgc
     8761 cgcggcgagc acggccttgc ggcgctggcc catcgagagc tggcgcaccg aacggtgggc
     8821 gagcgtcccc agctttacga agtcgagagc ctcctgcacc cgctcccgcg gctcgccgcg
     8881 cagccggcgg accagctcca ggatctcgcg cacggtggca tagggggtga ggtccggctg
     8941 ctccgacaga taggccaggc tccgccgcgc cgccacctcc tgggtccaga ggtcgtgccc
     9001 gctcacctcg acgctcccgg agtccggacg ctccaccccg gccgccatct tgagaagcgt
     9061 gctcttgccg cagccgttcg gccccaccag cagggtgagc cccggcccga tgtggacatc
     9121 gacttcggag agcaccggct cgcccggccc ataggagaag gagacttgat ggaagcggat
     9181 catggtcagg cgctccagga ctgcgaatcg cccacggcca ggtggtgcag ctccagccag
     9241 cggctcacct tctggcggcg ctcgcgctcc gccgcccagc ggaccgccag cggcgtcatc
     9301 gcgaggagcg gcaggacgac ccaggggagg agcgcggtgg ccgccaccgc cggcagcccc
     9361 tccagcagga tctcccccga cgcgcccgtg cgccgctccg gcgcccggcg cattgccccc
     9421 gcggcccgca gggcgaggaa cggcgccagg ccgaagagca ccagcgccat cgggtgaagg
     9481 agcacggcga ggatcaataa aggaagagcg tgaagggcca ggaaggcccc gtcggcgatc
     9541 acccgccctg tcgccgacca ggggagggag cgcgaccagg gccaggccgg ccgaaacgcg
     9601 gcaagcgcct cacccagctg ggcgaacagg aggacgaccg cgcagccccc tcccaggagg
     9661 ctccccagga tcacgtgctt cggcgccagc tcgttgttgg cgatgaacgc ccaggcagcg
     9721 cccaggggga ggagcgctcc gaagtaggcc gataccagct tccaacccag cgcccgccag
     9781 gcgatgcgtg cggagaagag ggcgagatcc agcgaagggc ttcctccccc tgggagggg
//
LOCUS       DASPJI010000574        13430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_384436, whole genome shotgun sequence.
ACCESSION   DASPJI010000574 DASPJI010000000
VERSION     DASPJI010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13430)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13430
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384436"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1490
                     /locus_tag="VEL74_16060"
     CDS             <1..1490
                     /locus_tag="VEL74_16060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG64090.1"
                     /translation="NAVARAARTGRPELLQDVHRLRRPCGEDGRDRGELADIYRALDR
                     QSSLILPLAARGQTVGVLFMATSVSGRSYGAEDLALGEDLARRAALAVDNGRLYREAQ
                     AANAAKDRFLATLSHELRTPLTPVLAVVSSLRDEKSLSDEVRSRLDMIRRNVELEARL
                     IDDLLDLTRIARGKLELRREPSSVESLVEHALETCQGEGTDDDRLQIEIHLAADDHLV
                     LADAPRLTQVFWNLLRNAFKFTPAGGAVRVHSWNEPPLPDSPGGTSGTVAVEIADTGM
                     GIDPGLLPRIFDAFEQGDRRVTRQFGGLGLGLAITKAIVELHGGTLSAHSDGEGLGAT
                     FTVRLPLAGTGALPLSLPLSPPEVTRRQPVAAPLPAEPLRLLLVEDHPDTADAMADLL
                     RCQGHQVIVARSVAAALGAVESEGAIDLVVSDLGLPDGSGLDLMRELAGRRGLRGIAL
                     SGYGTDEDVRRSLAAGFAEHLTKPVDFHTLKEAILRAAEPVVRVE"
     gene            1586..3400
                     /locus_tag="VEL74_16065"
     CDS             1586..3400
                     /locus_tag="VEL74_16065"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG64091.1"
                     /translation="MSSGEIPPSDPCDPFAAGGSAILELQRAKEAAERQARRLALLSD
                     VAARLLLDDHPEQLLAALFERISEELGLEVYLHFLVDPDGRSLTLSSCAGLPAAEIPG
                     LRRIEFGEAVCGTVALEQRGRVAEDVQASTEELHDLIRSMGITAYACHPLLCGGKLLG
                     TLSFGTRRRPRFETDELDLMRTICDQIATTVERSRLMDELRERGREAQEAREAAEAAS
                     RAKDRFLATLSHELRTPLTPLLLTVQALQTDRRMPDELRPGLERMRRNVEREARLIDD
                     LLDLTRIARGKVPLQCEVVDVHILLRHALDTCTAEAPGSRAEVVLEPLAGRSQVWADA
                     ARLEQVFWNLISNALKFTGPEGRVVVRTSNDSQGCVRVEVCDTGVGIEPDLLPHIFNA
                     FEQGGGQVTRRFGGLGLGLAISKSLVDLHKGTLQAASDGKGGGAVFTVTLRPLEHPEA
                     RVAAGPEPGTEDAEPPLHLLLVEDHADTADALAALLRGRGHRVTLAGSLATALERAAA
                     AQLDGEPVIDLVVSDLGLPDGTGYDLMRELVDRYGLRGIALSGYGMEDDLQKSEEAGF
                     DTHLTKPVSLEALEQAIRQVTREIVRGAGAARTYTPHP"
     gene            3397..4920
                     /gene="hutH"
                     /locus_tag="VEL74_16070"
     CDS             3397..4920
                     /gene="hutH"
                     /locus_tag="VEL74_16070"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HYG64092.1"
                     /translation="MIQIDGTSLTLEQFARAVQERVPCSLAPAARDRVRAARAVIERA
                     VDTGQVVYGVNTGFGDLANVRIDREQLQQLQRRLILSHAAGMGEPLPDVAVRGMLLLR
                     ANTLARGHSGVRPEVVEALLTLLNQDVLPVVPSRGSVGASGDLAPLAHLALPLLGRGR
                     VRAGGVEMTAEDGLRRAGLAPMELQAKEGLALINGTQAMAALLALAVLEARRLVRAAD
                     LIGALTTDALRGTDAAFDPRIHALRPHPGQRASAANLWRLMQGSAIRESHRVGDVRIQ
                     DPYSVRCMPQVHGAVRDVLADVETKLVVEMSSVTDNPLVFPAEEGGKEAVISGGNFHG
                     EPMAMAADVLAIALAELGSISERRIEKLTNAAFSGLPPFLVREAGLSSGFMIAQVTAA
                     ALVSENKGLAHPASVDSIPTSADKEDHVSMGMGAALKLQAVVDNVRRVLAIELVAAAQ
                     GIDLLRPLTSSLPLEALHATVRTRIQVWEEDREMASDLTMAEELLRGGLDLHLHGVE"
     gene            4987..6750
                     /locus_tag="VEL74_16075"
     CDS             4987..6750
                     /locus_tag="VEL74_16075"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYG64093.1"
                     /translation="MKRFPVHRSELLIGSDPDCDICLQYTGVAHQHARLRFDGNQLRI
                     EDLGSRKGVLVGGRKVRESALEVLDEIRLGNVTLLVEDVVPQPEKVAAPPVEQRAAVG
                     PPRITPERMMEHVLRISEWVLGDTESRTTSEALITVVLADFGGGALFLLLGEREEAGV
                     KLAVASDSAWLPSGVELMNQVEARMPAGGGEVAFGGSLAGAPAWICYHSFAALDRTYS
                     LVAAFPNFFPGAWSPLASLKTMGHLLVLGLVHHVGWYEPILPGHPGQQDLMLDPALVV
                     GESQVMKRVVEQLRGAVDPGVHVLLLGEPGVGKELLAKSLHLSGSRRNSPFVVASCSG
                     AKESQIEADLFGAEIQGKGEPLRREGKLLLADGGTIFLDDVDQLPIDLQGRLARFLRT
                     NEVEPRGSDHSHKVDVRLIAGSRAPLEAAVARDTFRVDLAYNLSQYTIEVPPLRDRRD
                     DLPLLIQSYINRFCHETGKRMQGITVKAMSSLLSYDYPGNLAELENVARQLVYQSMPG
                     RPVDVNLLPERVRNGTLSTAARVDPGSDLDLERLVGSCEQAAIREALRRTGGNKSQAA
                     RILGLSRNGLAIKMDRYGVRG"
     gene            7310..9316
                     /locus_tag="VEL74_16080"
     CDS             7310..9316
                     /locus_tag="VEL74_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HYG64094.1"
                     /translation="MKEDAGRQRVIIEGVEPEIDCGRFPIKRTIGETVVVEADLFTDG
                     HDSISALLLYKREKDSGWTEVPMTPLVNDRWRAELRITELGLWLYTVTGWVDHFKTWR
                     KDFRKRVDAGQDVAVDLLIGAELIRGASARALAAGRKGDARVLDGLASDLADSKDPRA
                     RIRLALDDELAQLMDRFPDRGFASVYPRELAVKADRERARFSAWYEMFPRSCGTGGRH
                     GTFRDCEERLPYIAGMGFDVLYLPPIHPIGQTFRKGKNNKVTAEPGDVGSPWAIGAEE
                     GGHKAIHPDLGTLDDFRRFLARAKEHGIELALDIAYQCAPDHPYVREHPEWFKRRPDG
                     TIQYAENPPKKYQDIYPFDFESDAWRGMWEELKSVVDFWIGEGVRIFRVDNPHTKPFP
                     FWEWMIGEVHREHPDVIFLAEAFTRPKIMYRLAKLGFTQSYNYFPWRNTRWELTEYLT
                     ELTQTEVQEYCRPNLWPNTPDILTEPLQIGGRPMFVQRFILAATLGASYGIYGPAYEL
                     MEHLPRERGSEEYLDSEKYQLREWDLGRSDSLRELISLVNRVRQDNPALQTNATLRFH
                     ATDNDKLICYSKTAEEAGGGNIVVVVVNLDPNYVQSGWVDLRLDELGLDREQSFQVHD
                     LLTGARYLWHGSRNFVQLDPRAVPAHIFRIRRRVRSERDFDYFM"
     gene            9348..12770
                     /gene="treS"
                     /locus_tag="VEL74_16085"
     CDS             9348..12770
                     /gene="treS"
                     /locus_tag="VEL74_16085"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HYG64095.1"
                     /translation="MSPRGDDRKRTEPEDDPLWYKDAIIYELHVRAFYDSDGDGRGDF
                     QGLTSKLDYLRELGVTAIWILPFYPSPWRDDGYDISDYNDVHPAYGSMEDFETFLAEA
                     HRRGLRVITELVVNHTSDQHPWFQRARLAPAGSPERDFYVWSDTDKKYAGTRIIFTDT
                     EASNWTKDPVADAYYWHRFFSHQPDLNYDNPLVQEELFKALDFWMEKGVDGLRLDAVP
                     YLYEREGTNNENLPETHEFLRKLRSHVDTRFRGRMLLAEANQWPEDAIAYFGDGDECH
                     MCFHFPVMPRLFMAIRLEDRVPIVDILEQTPPIPENAQWATFLRNHDELTLEMVTDED
                     RDYMYRVYAADKAQRINVGIRRRLAPLLGNDRRRIELMNGLLFSLPGTPVLYYGDEIG
                     MGDNFYLGDRNGVRTPMQWSPDKNAGFSRTNPQRLYLPVIVDPTYHYESVNVEAQQSN
                     PYSLLWWTRRLIALRKRHKAFSRGTLEFLHPENYRVLAFVRRYEGETLLVVANLSRFV
                     QHAELDLSALDGLTPVELFGRNRFPAIRAGRPYPMTLGPHAFYWFSLEQEPALVGPRG
                     DTAPGEAAAPLAVLPVAGAWTSVLAGEARADLGAALAALLPSRRWFGGKARTIRAVTV
                     GSVVPLAREEETNAPASYLALLNVEYTDGEPETYALPLAFVEGPRAERLATATPPVAV
                     AWVEAGGSRGLLYEPVGEPWFWSRLLRDIGERQRVRGDRGEVVAWPTAVFAEKGGHEG
                     NPEPVLLKAEQSNTSVRFGDRFVLKLFRRLESGVNPDLEIGLFLTEKTTYRCSPRVAG
                     GLEVRVTRGEEPLTLGILQEFVPNEGDAWRFTLDALGRYFDRVRTDWGGGGEHQMATM
                     PTEPLLELASRPPGDEVAERIGTYLPLVRLLGERTAELHIALASAGPDARDFAPEPFS
                     ELYQRSLYESMRGLTRKNFRLLRHNLGNLPDDVRTDAEVVLAAEERVIETFRRVSGPK
                     MAAERIRIHGDFHLGQVLYTGKDFVILDFEGEPTRSLSERRLRRSPLRDVAGMLRSFQ
                     YATYARLLEEQEAGGVPPEEMERFQSWAFYWERWVSAAYLQAYLDRMGTAAILPPSRE
                     HLSLLLDAYVLEKAVYELSYELNNRPAWVRIPLQGIRQILGASESTGGRGGE"
     gene            12760..>13430
                     /locus_tag="VEL74_16090"
     CDS             12760..>13430
                     /locus_tag="VEL74_16090"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF045011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HYG64096.1"
                     /translation="MASEAEDLKSLAGLYGVQTSYWDAMGRHVEASPDSLLAALAALE
                     APVERREDVPEALAARHEELADRLIEPVVVAWDGHAPEIELRTRGGGSAGLHLDLEGG
                     ERRGWMVDLDSLPGRRLSLPEPLPPGYHRLRVEVNGRSAETLVISAPSRAFTGDGRPL
                     WGVFLPLHALHTSRSWGAGDFSDLEALAEWTASLGGGVVGTLPMLSSFLDEPFEPSPY
                     APASRL"
BASE COUNT         1967 a         4833 c         4686 g         1944 t
ORIGIN      
        1 acaacgccgt ggcccgggcc gcgcgcaccg gccggccgga gctgctccag gacgtgcacc
       61 ggctccgccg cccctgcggg gaggacggcc gggaccgggg ggagctggcg gacatctatc
      121 gtgccctcga ccggcagtcg agcctgatcc tgccgctggc cgcccgcggc cagaccgtgg
      181 gggtgctgtt catggccacc tcggtgtcgg ggaggagcta cggcgcggag gatctggcgc
      241 tcggcgagga tctggcgcgg cgggcggcgc tggcggtgga caacggccgc ctctaccgcg
      301 aggcgcaggc ggccaacgcc gccaaggacc gcttcctcgc caccctgtcg cacgagctgc
      361 ggaccccgct caccccggtc ctcgccgtgg tttccagcct gcgcgacgag aagagcctga
      421 gcgacgaggt gcggagccgg ctcgacatga tccgccgcaa cgtcgagctg gaggcgcggc
      481 tgatcgacga cctgctggac ctcacgcgca tcgcccgcgg caagctggag ctgcggcggg
      541 agccgagcag cgtcgagagc ctggtggagc atgcgctgga gacctgccag ggggagggga
      601 cggacgacga ccggctgcag atcgagatcc acctggcggc ggacgaccat ctcgtcctgg
      661 cggacgctcc gcggctcacc caggtgttct ggaacctgct gcgcaacgcc ttcaagttca
      721 cccccgccgg cggtgccgtc cgggtgcact cctggaacga gccgcccctc ccggacagcc
      781 cgggcgggac gtctggaacg gtggccgtgg agatcgccga caccgggatg gggatcgatc
      841 ccggcctgct gccgcggatc ttcgacgcct tcgagcaggg ggaccggcgg gtgacgcgcc
      901 agttcggcgg cctcgggctc gggctcgcca tcaccaaggc catcgtggag ctgcacggcg
      961 gcaccctgtc cgcgcacagc gacggcgagg gcctgggcgc gacgttcacc gtccgcctgc
     1021 cgctcgccgg gaccggcgcc ctgccgctct ctctgcccct gtcgccgccg gaggtgaccc
     1081 gccggcagcc ggtggccgcg ccgctcccgg cggagcccct gcgcctcctc ctcgtcgagg
     1141 accaccccga caccgccgac gccatggccg acctgctgcg ctgccagggg caccaggtca
     1201 tcgtggcccg ctccgtggcg gcagccctcg gcgcggtgga gagcgagggc gccatcgacc
     1261 tggtggtgag cgacctgggg ctgcccgacg gcagcggcct cgacctgatg cgcgagctgg
     1321 ccggccgccg gggcctccgc gggatcgccc tctccggcta cggtaccgac gaggacgtgc
     1381 gccggagcct ggccgccggc ttcgccgagc acctcaccaa accggtcgat ttccacaccc
     1441 tcaaggaggc gatcctccgc gccgccgagc ccgtggtccg cgtggaatag agcctctgca
     1501 tacgctgttg tcggagcgtc gtcttttaca gggcgcgcag cgcaggcgcc acctccctta
     1561 actacagctt ctcggaggct ggcggatgag ctccggcgag atcccaccct cagacccgtg
     1621 cgacccgttt gccgctggcg gctcggcgat cctcgagctg cagcgggcca aagaggccgc
     1681 cgagcggcag gcgcggcgtc tggcgctgct ctcggacgtc gcggcccggc tcctgctcga
     1741 cgatcacccc gagcagctcc tggcggcgct gttcgagcgg atctcggaag agctcgggct
     1801 cgaggtctac ctgcacttcc tggtggaccc ggacggccgg tccctgactc tctcgtcctg
     1861 tgccgggctg ccggcggcgg agatccccgg gctccggcgc atcgagttcg gcgaagcggt
     1921 ctgcggcacc gtcgcactgg agcagcgagg ccgggtggcg gaggatgtcc aggcctccac
     1981 cgaggagctc catgacctga tccgctccat ggggatcacc gcctacgcct gccaccccct
     2041 cctctgcggc ggcaagctcc tggggactct ctccttcggc actcgccggc ggccgcgctt
     2101 cgagaccgac gagctcgacc tgatgcggac gatctgcgac cagatcgcca ccacggtgga
     2161 gcgctcccgt ctgatggacg agctccggga gcgcggccgc gaggcacagg aggcgcggga
     2221 ggcggcggag gccgccagcc gggccaagga ccggttcctc gccaccctca gccatgagct
     2281 gcggacgccg ctgaccccct tgctgctcac cgttcaggcc ttgcagaccg accgccggat
     2341 gcctgacgag ctgcgccccg ggctggagcg gatgcgccgc aacgtggaac gggaggcgcg
     2401 gctcatcgac gacctcctgg acctgacgcg gatcgcgcgc ggcaaggtcc ccctccagtg
     2461 cgaggtggtg gacgtccata tcctgctgcg ccacgccctc gacacctgca ccgccgaagc
     2521 cccggggagc cgggcggagg tggtgctcga gccgctcgcc gggcggagcc aggtgtgggc
     2581 cgacgcggcc cgcctggagc aggtgttctg gaacctgatc agcaacgccc tcaagttcac
     2641 cggccccgag gggcgcgtcg tcgtccggac gtccaacgac tcccagggtt gcgtgcgggt
     2701 ggaggtgtgc gacaccggcg tcggcatcga gccggacctc ctgccgcaca tcttcaacgc
     2761 cttcgagcag gggggcggtc aggtcacccg gcgcttcggc ggcctcgggc tggggcttgc
     2821 catcagcaag agcctggtgg acctgcacaa gggcaccctc caggcggcca gcgacgggaa
     2881 gggagggggc gccgtgttca ccgtgacgct gcgcccgctg gagcaccccg aagcccgggt
     2941 ggcggccggg ccggagccgg ggaccgagga cgccgagccg ccgctccacc tcctcctggt
     3001 agaggaccac gccgacaccg ccgacgccct ggcggcgctg ctgcgcggcc gcggccacag
     3061 ggtgaccctg gccggcagtc tggcgacggc cctggagcgg gcggccgcgg cgcagctcga
     3121 cggcgagccg gtcatcgatc tggtggtgag cgacctgggg ctgcccgacg gcaccggcta
     3181 cgacctgatg cgcgagctgg tcgaccgcta cggcctgcgg gggatcgcgc tctccggcta
     3241 tggcatggag gacgacctcc agaagagcga ggaggccggc ttcgacaccc atctgaccaa
     3301 gccggtcagc ctggaggccc tggagcaggc gatccgtcag gtcacccgtg agatcgtgcg
     3361 aggggcgggc gcggcgcgga catatactcc gcacccatga ttcagatcga cggcacctcc
     3421 ctcaccctgg agcagttcgc ccgcgccgtc caggagcgcg tcccctgctc gctcgccccc
     3481 gccgcccggg accgcgtccg cgccgcccgc gcggtcatcg agcgggcggt ggacaccggc
     3541 caggtggtct acggcgtcaa caccggcttc ggcgacctcg ccaacgtgcg catcgatcgc
     3601 gagcagctcc agcagctcca gcgccgtctg atcctttccc acgccgccgg catgggcgag
     3661 ccgctgcccg acgtggcggt gcgcggcatg ctgctgctgc gcgccaacac cctggcccgc
     3721 ggccactcgg gggtgaggcc cgaggtggtg gaggccctcc tgacgctgct caaccaggat
     3781 gtgctgccgg tggtgccgag ccgcggctcg gtgggcgcct cgggggatct ggcgccgctg
     3841 gcccatctcg ccctgcctct gctggggcgg ggccgggtcc gggcgggcgg cgtcgagatg
     3901 accgccgagg acggcctgcg ccgggcgggg ctcgcgccga tggagctcca ggccaaggag
     3961 gggctggcgc tgatcaacgg cacccaggcc atggccgcgc tcctcgccct ggcggtgctg
     4021 gaggcccggc gcctggtgcg cgccgccgac ctcatcggcg cgctcaccac cgacgcgctg
     4081 cgcggcaccg acgcggcgtt cgacccccgc atccacgccc tgcgcccgca tccgggccag
     4141 cgcgccagcg ccgccaacct ctggcggctg atgcaggggt cggcgatccg cgagtcccac
     4201 cgggtgggcg acgtgcgcat ccaggacccc tactcggtcc gctgcatgcc gcaggtgcac
     4261 ggtgcggtgc gcgacgtgct ggccgacgtc gagaccaagc tggtggtgga gatgagctcg
     4321 gtcaccgaca acccgctggt cttcccggcg gaggaggggg gcaaggaggc ggtgatctcg
     4381 ggcggcaact tccacggcga gcccatggcc atggccgcgg acgtcctcgc catcgcgctg
     4441 gccgagctgg gctcgatcag cgagcggcgc atcgagaagc tcaccaacgc cgccttctcg
     4501 ggattgcccc ccttcctggt gcgggaggcc ggcctgagct ccggtttcat gatcgcccag
     4561 gtcacggcgg cggctctggt cagcgagaac aaggggctgg ctcatcccgc ctcggtggac
     4621 agcatcccca cctcggcgga caaggaggac cacgtctcca tgggcatggg ggcggcgttg
     4681 aagctccagg ccgtggtgga caacgtgcgc cgggtgctgg ccatcgagct ggtggccgcg
     4741 gcgcagggga tcgacctgct gcggcccctc acctccagcc tgccgctgga ggcgctccac
     4801 gccaccgtcc gtacccgcat ccaggtctgg gaggaggacc gcgagatggc gtccgacctc
     4861 accatggccg aggagctcct acgcggcggc ctcgacctgc acctccacgg cgtcgagtag
     4921 gggccgcggc accccgtacg gtagacttcc cggatgcttc gcctggtcgc atacgcgtcg
     4981 gatggcgtga aacgctttcc cgttcaccgg agcgagctgc tcatcggcag cgaccccgat
     5041 tgtgacatct gtctccaata cacgggggtg gcgcatcagc acgcgcgcct ccgtttcgac
     5101 ggcaaccagt tgcggatcga ggacctgggg agccgcaagg gggtgctggt gggcgggcgc
     5161 aaggtgcggg agagcgccct ggaggttctg gacgagatcc ggctgggcaa cgtgacgctg
     5221 ctggtggagg acgtggtccc gcagcccgag aaggtggcgg cgccgccggt ggagcagcgg
     5281 gctgcggtgg ggccgccgcg gatcacgccg gagcggatga tggagcacgt cctccgtatc
     5341 agcgagtggg tgctgggcga cacggagagc cgcaccacct ccgaggccct gatcaccgtc
     5401 gtgctggccg atttcggcgg cggcgccctc ttcctgctgc tgggtgagag ggaggaggcg
     5461 ggtgtcaagc tggcggtggc gagcgattcg gcctggctgc ccagcggcgt agagctgatg
     5521 aatcaggtcg aggcccggat gcccgcgggc ggcggcgagg tggctttcgg cggcagcctg
     5581 gccggcgcgc ccgcctggat ctgctatcac tccttcgcgg ccctcgaccg gacgtactcg
     5641 ctggtggccg ccttccccaa cttctttccc ggtgcctggt cgccgctggc ctcgctcaag
     5701 accatggggc acctgctggt gctcggcctc gttcaccacg tcggctggta cgagccgatc
     5761 ctgcccggcc atccggggca acaggacctg atgctcgacc ccgcgctggt agtgggggag
     5821 tcgcaggtga tgaagcgggt ggtcgagcag ctccgagggg cggtggaccc gggggtgcac
     5881 gtgctgctgc tcggcgagcc cggcgtcggc aaggagctgc tcgccaagtc gctccacctg
     5941 tcgggcagcc ggcggaacag tccgttcgtc gtcgcgagct gcagcggcgc gaaggagtcg
     6001 cagatcgagg cggacctctt cggcgccgag atccagggga agggggagcc gctgcggcgc
     6061 gaaggcaagc tcctcctggc ggatggcggc acgatcttcc tggacgatgt ggaccagctc
     6121 cccatcgacc tccaggggcg cctcgcccgc ttcctgcgca ccaacgaggt ggagccgagg
     6181 gggagcgacc attcccacaa ggtcgacgtg cggctcatcg ccggcagccg cgcgccgctg
     6241 gaggcggcgg tggcccgcga caccttccgc gtcgacctgg cctacaacct ctcccagtac
     6301 accatcgagg tgccgccgct gcgcgaccgg cgggacgacc tgccgctgct gatccagtcc
     6361 tacatcaacc ggttctgtca cgagacgggc aagcggatgc aggggatcac ggtgaaggcg
     6421 atgtcgtcgc tcctctccta cgactatccg ggcaacctgg cggagctgga gaacgtggcg
     6481 cggcagctcg tctaccagag catgccgggc cggccggtgg acgtgaacct gctccccgag
     6541 cgggtgagga acggcacgct ctccaccgcc gcccgggtcg atcccggctc ggacctcgat
     6601 ctcgagcgcc tggtgggctc ctgcgagcag gcggcgatcc gcgaggccct gcgccgcacc
     6661 ggcggcaaca agtcccaggc cgcccgcatc ctcggcctct cccgcaacgg cctggcgatc
     6721 aagatggatc gctacggggt gcgggggtag ggccgctttt cggcatcaga tttgcaatag
     6781 ctctcaaacg cggcggccgc gtcgtgtcct gggaatcgca gcgacgtgag ccttcggagt
     6841 agctcgaacg cgtggggtga tgggcggggc tctccaccca ctccgacaaa gcgaagatcg
     6901 gcctcgcccc ggcccgccgc atactgctga ttccgatccc agtccccttc tgcaagccgc
     6961 gggcgccgga tctcgcccgg acgccgcggg cgccttccgg acggtccggg aagcgtccga
     7021 gcgcttcgga tcacgtccgg acgctctgga tctcatccac aaggcccggg aagctcccga
     7081 gcgctgcgga ccgcgtccga acgccccgga ttcaatccag gcgcttcgga agcttccctg
     7141 acgctccgga aggcgtccgg atcgtccgga tcgcatccac ggcgtccgga gcccttgacg
     7201 cgagtgatct ccagcccctc cccctttcac ctgctgtcca actctggtaa accttgcacc
     7261 cgcgccgggc gtggcaggtg gcctgcatgg cggggatcgg gaggtgaaga tgaaagagga
     7321 cgcgggcagg cagagggtca tcatcgaggg ggtcgagccg gagatcgact gcgggcggtt
     7381 ccccatcaag cggaccatcg gcgagacggt ggtggtcgag gccgacctgt tcaccgacgg
     7441 ccacgactcc atctccgccc tgctgctcta caagcgggag aaggacagcg gctggaccga
     7501 agtccccatg accccgctgg tcaacgaccg ctggcgggcg gagctcagga tcaccgagct
     7561 ggggctctgg ctctacaccg tcaccggctg ggtcgaccac ttcaagacct ggcgcaagga
     7621 tttccgcaag cgcgtcgacg ccggccagga cgtcgccgtc gacctgctga tcggcgcgga
     7681 gctgatccgc ggcgccagtg cccgcgccct ggcggcaggc cgcaaggggg acgcccgcgt
     7741 gctcgacggc ctcgcctccg atctcgccga tagcaaggac cccagggccc gcatccgcct
     7801 cgcgctggac gacgagctcg cccagctcat ggaccgcttc ccggaccgcg gcttcgcctc
     7861 cgtctatccg cgcgagctgg cggtcaaggc cgaccgcgag cgggcgcgct tctccgcctg
     7921 gtacgagatg ttcccgcgct cgtgcggcac cggcggccgg cacggcacct tccgcgactg
     7981 cgaggagcgc ctgccctata tcgccggcat gggcttcgac gtgctctacc tgccgcccat
     8041 ccaccccatc ggccagacct tccgcaaggg caagaacaac aaggtcaccg ccgagcccgg
     8101 cgacgtgggg agcccgtggg ccatcggcgc cgaggagggg gggcacaagg cgatccatcc
     8161 ggacctcggg accctcgacg acttccggcg gttcctcgcc cgcgccaagg agcacggtat
     8221 cgagctggcg ctggacatcg cctaccagtg cgcgcccgac cacccctacg tgcgcgagca
     8281 tccggagtgg ttcaagcggc gccccgacgg caccatccag tacgccgaga acccgcccaa
     8341 gaagtaccag gacatctacc cgttcgactt cgagagcgac gcctggcgcg ggatgtggga
     8401 agagctcaag agcgtcgtcg acttctggat cggcgagggg gtgcgcatct tccgcgtcga
     8461 caacccgcac accaaaccgt tccccttctg ggagtggatg atcggcgagg tgcaccgcga
     8521 gcatcccgac gtgatcttcc tggccgaggc cttcacccgg ccgaagatca tgtaccggct
     8581 ggccaagctg ggcttcaccc agtcttacaa ctacttcccc tggcgcaaca cccgctggga
     8641 gctgaccgaa tacctgaccg agctcaccca gaccgaggtg caggagtact gccggcccaa
     8701 cctctggccc aacacgccgg acatcctcac cgagccgctg cagatcggcg gccggcccat
     8761 gttcgtgcag cgcttcatcc tcgccgcgac cctgggggcg agctacggca tctacggccc
     8821 ggcctacgag ctgatggagc acctgccgcg cgagcggggg agcgaggagt acctggactc
     8881 cgagaagtac cagctccgcg aatgggacct cggccggtcc gacagcctgc gcgagctgat
     8941 ctccctggtc aaccgcgtcc gccaggacaa cccggcgctg cagaccaacg ccaccctgcg
     9001 gttccacgcc acggacaacg acaagctgat ctgctacagc aagaccgccg aggaggccgg
     9061 tggcggcaac atcgtcgtcg tggtggtcaa cctggatccc aactacgtcc agtcgggctg
     9121 ggtggacctg cgcctcgacg agctggggct cgaccgcgag cagtccttcc aggtgcacga
     9181 cctgctcacc ggcgcccgct atctctggca cggttcgcgc aacttcgtgc agctcgatcc
     9241 gcgggcggtg cccgctcaca tcttccgtat ccggcggcgc gtccgctcgg aacgtgactt
     9301 cgactatttc atgtagctgt tggaatgccc gtcaggaaag gggagccatg agcccgagag
     9361 gcgacgacag gaagaggaca gagccggagg acgatccgct ctggtacaag gacgcgatca
     9421 tctacgagct gcatgtccgc gcgttctacg actcggacgg cgacggccgc ggcgatttcc
     9481 agggcctgac ctcgaagctg gattacctgc gggagctggg cgtcacggcc atctggatcc
     9541 tgccgttcta tccctccccc tggcgtgacg acggctacga catctccgac tacaacgacg
     9601 tgcatccggc ctacggctcg atggaggact tcgagacctt cctggccgag gcccaccgcc
     9661 gcggcctgcg ggtgatcacc gagctcgtgg tcaatcacac ctcggaccag cacccgtggt
     9721 tccagcgcgc ccgcctggcg ccggcgggca gcccggagcg cgacttctac gtctggagcg
     9781 acaccgacaa gaagtacgcc ggcacgcgca tcatcttcac cgacaccgag gcctccaact
     9841 ggaccaagga cccggtggcc gacgcctact actggcaccg cttcttctcc caccagccgg
     9901 acctcaacta cgacaacccc ctggtgcagg aggagctctt caaggccctg gatttctgga
     9961 tggagaaggg ggtcgacggc ctgcgcctcg acgccgtccc ctacctctac gagcgcgagg
    10021 ggaccaacaa cgagaacctg cccgagacgc acgagttcct gcgcaagctc cgctcccacg
    10081 tggacacgcg gttccgcggc cgcatgctcc tcgccgaggc caaccagtgg cccgaggacg
    10141 ccatcgccta cttcggcgac ggcgacgagt gccacatgtg cttccacttc ccggtcatgc
    10201 cccgcctctt catggccatc cgcctggagg accgggtgcc gatcgtcgac atcctggagc
    10261 agacgccgcc catccccgag aacgcgcagt gggcgacctt cctgcgcaac cacgacgagc
    10321 tgaccctgga gatggtcacc gacgaggacc gggactacat gtaccgggtg tacgccgcgg
    10381 acaaggcgca gcgcatcaac gtcggcatcc gccgccgcct ggcgccgctg ctgggcaacg
    10441 accgtcggcg catcgagctg atgaacggcc tgctcttctc gctgcccggc acgccggtcc
    10501 tctactacgg ggacgagatc gggatggggg acaacttcta tctcggcgac cgcaacggcg
    10561 tgcgcacccc catgcagtgg agcccggaca agaacgccgg cttctcgcgc accaacccgc
    10621 agcggctcta cctgccggtg atcgtcgacc cgacctatca ctacgagtcg gtcaacgtcg
    10681 aggcgcagca gtccaacccc tactcgctcc tctggtggac caggcggctc atcgcgctgc
    10741 gcaagcggca caaggcgttc agccgcggca ccctcgagtt cctccacccg gagaactacc
    10801 gggtgctcgc cttcgtccgc cgctacgagg gggagaccct gctggtggtg gccaacctgt
    10861 cgcgcttcgt gcagcacgcg gagctggacc tgtcggccct ggacggcctg acgccggtgg
    10921 agctgttcgg ccgcaaccgt ttccctgcca tccgcgccgg ccgcccctat ccgatgacgc
    10981 tggggccgca tgccttctac tggttctcgc tggagcagga gcccgccctg gtcgggccgc
    11041 gaggcgacac ggcgcccggc gaggccgccg cgccgctcgc cgtgctcccg gtcgccggcg
    11101 cctggacgtc cgtcctcgcg ggggaggcgc gcgccgacct cggagcggcg ctcgccgccc
    11161 tcctgccgtc gcggcgctgg ttcggcggca aggcgcgcac catccgcgcc gtgacggtgg
    11221 gcagcgtcgt tcccctggct cgggaggagg agacgaacgc acccgcctcc tacctggctc
    11281 tcctgaacgt cgagtacacc gacggcgagc ccgagaccta tgccctgccg ctcgccttcg
    11341 tggagggtcc gcgggccgag cggctggcca ccgcgacgcc gcccgtcgcc gtggcctggg
    11401 tcgaggcggg gggctcccgc ggcctgctgt acgagccggt gggggagccc tggttctgga
    11461 gccggctgct ccgcgacatc ggcgagcggc agcgggtccg cggcgaccgg ggcgaagtcg
    11521 tggcctggcc caccgccgtc ttcgccgaga aggggggcca cgagggcaac cccgagccgg
    11581 tcctcctcaa ggcggagcag agcaacacct cggtgcgctt cggcgaccgc ttcgtcctca
    11641 agctcttccg gcgtctcgag tccggagtca atccggacct ggagatcggc ctcttcctga
    11701 ccgagaagac cacctatcgc tgctcaccgc gggtggccgg cggcctcgag gtccgcgtca
    11761 cccgcggcga ggagccgctg accctcggca tcctgcagga gttcgtgccc aacgaggggg
    11821 acgcctggcg cttcaccctc gacgccctcg gccgctactt cgaccgggta cgcacggact
    11881 ggggcggcgg cggcgagcac cagatggcca cgatgcccac ggagccgctg ctcgagctgg
    11941 cctcccggcc gccgggcgac gaggtggcgg agcggatcgg cacctacctg ccgctggtcc
    12001 gcctgctggg tgagcgcacc gccgagctgc acatcgccct ggcctcggcc gggccggacg
    12061 ccagggactt cgcgccggag cccttctccg agctctacca gcggtcgctc tacgaatcga
    12121 tgcgggggct cacccgcaag aatttccggc tgcttcgcca caatctgggc aatctcccgg
    12181 acgacgtgcg gaccgacgcc gaggtggtgc tggcggccga ggagcgggtg atcgagacct
    12241 tccgccgcgt ctcgggcccg aagatggcgg ccgagcgtat ccgtatccat ggcgacttcc
    12301 acctcggcca ggtgctctac accggcaagg acttcgtcat cctcgatttc gagggggagc
    12361 cgacgcgctc gctgtcggag cggcgcctca ggcggtcccc cctgcgcgac gtcgccggca
    12421 tgctccgctc gttccagtac gcgacctatg cgagactgct ggaggaacag gaggcggggg
    12481 gcgtgccgcc ggaggagatg gagcggttcc agagttgggc cttctattgg gagcgctggg
    12541 tctcggccgc gtatctccag gcgtacctgg accgcatggg gacggccgcc atcctccccc
    12601 cgtcccgcga gcacctgtcg ctgttgctcg acgcctacgt gctggagaag gcggtctacg
    12661 agctgagcta tgagctgaac aatcgccccg cctgggtccg gatccctctc caggggatcc
    12721 gccagatcct cggggcgtcc gagtcgacgg ggggacgcgg tggcgagtga agccgaagac
    12781 ctgaaatccc tcgctgggct ctacggcgtc cagacctcct attgggacgc catgggccgc
    12841 cacgtggagg cgtctccaga ctcgctgctc gcggccctgg ccgccttgga ggcaccggtg
    12901 gagcggcggg aggacgtgcc ggaggccctc gcggcccgcc acgaggagct ggcggaccgc
    12961 ctcatcgagc cggtggtggt cgcctgggac ggtcacgctc cggagatcga gctgcgcact
    13021 cgaggcggcg gctccgccgg cctgcacctc gacctcgagg ggggagagcg gcggggatgg
    13081 atggtggacc tggacagcct gccggggcgc cgcctgtcgc tgcccgagcc cctgccgccg
    13141 ggctatcacc ggctgcgggt ggaggtgaac ggccgctccg ccgagacgct ggtgatctcg
    13201 gccccgtcgc gcgccttcac cggcgacggc cgtcccctct ggggcgtctt cctgccgctc
    13261 cacgcgctgc acacctcccg gagctggggg gccggcgact tctccgacct cgaggccctc
    13321 gccgagtgga cggcgagcct gggcggagga gtggtgggca ccctgccgat gctgtcgtcc
    13381 ttcctcgacg agccgttcga gcccagcccg tacgcgccgg ccagccgcct
//
LOCUS       DASPJI010000575         5082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_783991, whole genome shotgun sequence.
ACCESSION   DASPJI010000575 DASPJI010000000
VERSION     DASPJI010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783991"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VEL74_16095"
     CDS             complement(<1..654)
                     /locus_tag="VEL74_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64097.1"
                     /translation="MLNSAAVRLRTLLLAGAPFLMGALLFPLAACRARDGVAPSAAPA
                     ASGSWERVDLWSKPAEMEMVPPEAGGAAGAPAVIARMSFLGAEEVRDMRLVAPQQLAA
                     FPRRTAGQVRALEQMAGSRFHWRVRPGREGYLSFIPLGSPAGCPCTYRAGVREAEGKV
                     SELFRMDAEPMGPIAPAAVELDLSEYAGRDIELLLQVDGPTPLPPGQPVPTLLWGSPA
                     "
     gene            complement(660..2888)
                     /locus_tag="VEL74_16100"
     CDS             complement(660..2888)
                     /locus_tag="VEL74_16100"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG64098.1"
                     /translation="MDLRAKLARHRKNNRLIIGALVVLLFLFTGTFYWTQQGRDLPSY
                     LVTNRVLLFVLWYTNVVLILTILFVLLRNVVKLLLERRHRILGSTFKFKLVATYVGLS
                     LIPVLLLFAIATELLQGSIDRWFNTPVRPVLERGNAVAQALYDQIERTHLRDAGRVLA
                     AIEGFDLREVEQRPSLNRRLQELQTELGLDVLAVYDGTDFVQAVLNPRSGIPDLPEPG
                     RDFLEEAAEEGEATRVQPPPGGQGQLVLAAVAAAASPREPTIEEEPPPIVVVVAGSLL
                     DPALAERTERLVEDFQTYRQIEVQKEDLRASHLLIFLMVTLLILLASSWVGLYLARRV
                     TIPIQALAEGTRRISGGDLSHRVDVEADDELGVLVESFNRMTQELASNKELLERGNQE
                     LVSTNQRLAGARARIAAVLENVAAGVVSVDSDGRILTCNGAALAMLRQREEDVLGRTV
                     GEAWGDPERAKLAAILAQAGQGTVTHVNKEVHLSLGGEIKTFDAKVTALRDAGGAPGR
                     VLVLEDLTELIKAQQLAAWNEAARRIAHEIKNPLTPIRLSAERLQMKSRQGDPGLGKA
                     IEEGVEIIVREVTTLQGMVDEFSRYARMPRPRPVQTDFARLVDETVHLYRDLKHGVEV
                     EGRIDPTAARAWIDAEQIKRALINLLDNAVEATDAPGRVTVSADANNGHLEIRVADTG
                     RGIPPAAKDKLFLPYFSTKGRGTGLGLAIVHRIITDHQGLIRVEDNVPRGTVFTIELP
                     TE"
     gene            complement(2898..3446)
                     /locus_tag="VEL74_16105"
     CDS             complement(2898..3446)
                     /locus_tag="VEL74_16105"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HYG64099.1"
                     /translation="MRAVLAFLCLAFLAGRAGADARITGLQVAVDGNRVLASLKLRDA
                     FDRRMVARLESGLRTTIQYRFELHSDRKRWYDNRLSASTLEVDALYDAVSRSYTVHSR
                     LDGELVESRTVRDLEALEAAMTQLERIPIFTLDAPPSRRRLLLKARAEMGSRLLFSFI
                     PVTIATDWADSNKFRYPPRQQP"
     gene            complement(3530..3805)
                     /locus_tag="VEL74_16110"
     CDS             complement(3530..3805)
                     /locus_tag="VEL74_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HYG64100.1"
                     /translation="MEAGMGLIAWVVLGLIAGLIAKALMPGKDPGGCVITVIIGIVGA
                     LLGGFLATALGFGGISGFDLRSIFIAILGAFILLAIWRMIRGRGRRT"
     gene            3927..>5082
                     /locus_tag="VEL74_16115"
     CDS             3927..>5082
                     /locus_tag="VEL74_16115"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HYG64101.1"
                     /translation="MSETRPRLYLIDGYSNIFRAYYAIRNLSNSRGEPTNAVYGFVTM
                     LRKLLREDKPDLIGVALDVGKTFRSEKFEDYKANRAPMPEDLASQMPLVRRAIEAHRI
                     PILELQGYEADDVLGALACKAADAGYHVVMVSADKDLMQLVSPHISLFHTGRNKMYGP
                     QEVTEDFGLPPEKVADVLALMGDSVDNIPGVPGIGEKGAKSLIQEYGSLEELLARAGE
                     VSRKAYREGLQQHAEQARLSKELSTIHCDLPIPFDPEALRHDPPDNDALRQLYTELEF
                     FSLVEELKREPAADAAERVPPATELASAAAWHRQTAALGQEDGEVFVAVLGEERPLGL
                     AVGGPETDEVLYADFRRGDDLRAAALATLGRWVDNPTVRLSGHNLKEVLRL"
BASE COUNT          761 a         1754 c         1848 g          719 t
ORIGIN      
        1 cgccgggctc ccccagagga gggtggggac cggctgacca ggggggaggg gagtcgggcc
       61 gtccacctga agcagcagct cgatgtcgcg gccggcgtac tcggagaggt cgagctccac
      121 ggcggccggg gcgatggggc ccatgggctc ggcgtccatc cggaacagct cgctgacctt
      181 gccctccgcc tcccgcaccc ctgcccggta ggtgcagggg caacccgcgg gcgagcccag
      241 ggggatgaag gagaggtagc cctcccggcc cggccgcacc cgccagtgga agcgggagcc
      301 ggccatctgc tccagggccc gcacctgccc ggcggtccgc cgcgggaagg cggcgagttg
      361 ctggggcgcc accaggcgca tgtcccgtac ctcctcggcc ccgaggaacg acatccgggc
      421 aatgaccgcc ggcgcgccgg ccgcgccccc cgcctcgggg gggaccatct ccatctccgc
      481 cggcttgctc cacaggtcga cccgctccca ggaaccggac gccgcgggcg ccgcggacgg
      541 tgccacgccg tcgcgcgccc ggcaggccgc gagcgggaag agcagggcgc ccatcaggaa
      601 cggagcgccg gcgaggagga gggtccggag tcgcacggcg gcggagttta gcaccggccc
      661 tactcggtcg gcagctcgat ggtgaacacc gtgccccgcg gcacgttgtc ctcgacccgg
      721 atcaggccct ggtggtcggt gatgatgcgg tggacgatgg ccagcccgag gccggtgccc
      781 cgccccttgg tcgagaagta ggggaggaag agcttgtcct tggccgccgg agggatgccg
      841 cgcccggtgt ccgccacccg gatctcgaga tgaccgttgt tcgcgtctgc cgagaccgtg
      901 acccggcccg gggcgtcggt ggcctcgacg gcgttgtcca gcaggttgat cagggcgcgc
      961 ttgatctgct cggcgtcgat ccaggcgcgg gcggcggtgg gatcgatccg cccctccacc
     1021 tcgacgccgt gcttcaggtc ccggtagagg tggaccgtct cgtcgaccag ccgcgcgaag
     1081 tccgtctgga ccggacgcgg ccggggcatg cgggcgtacc gggagaactc gtccaccatc
     1141 ccctggagcg tggtcacctc gcggacgatg atttcgacgc cctcctcgat cgccttgccc
     1201 aggccggggt ccccctgccg ggacttcatc tgcaggcgct cggcggagag gcggatgggg
     1261 gtcagggggt tcttgatctc gtgggcgatg cgccgcgccg cttcgttcca ggcggcaagc
     1321 tgctgcgcct tgatgagctc ggtgaggtcc tccagcacca gcacccggcc cggcgctccg
     1381 ccggcgtcgc gcagcgccgt gaccttggcg tcgaaggtct tgatctcgcc gcccaggctc
     1441 aggtggacct ccttgttgac gtgggtgacc gtcccctggc cggcctgggc gaggatggcg
     1501 gccagcttgg cgcgctcggg gtcgccccag gcctccccga cggtccggcc gagcacgtcc
     1561 tcctcgcgct ggcgcagcat ggcgagcgct gcgccgttgc aggtgaggat gcggccgtcc
     1621 gaatccaccg acacgacgcc ggcggcgacg ttctccagca cagcggcgat gcgtgcccgc
     1681 gccccggcca ggcgctggtt ggtgctgacc agctcctggt tgccgcgctc cagcagctcc
     1741 ttgttggagg cgagctcctg ggtcatgcgg ttgaacgact ccaccagcac cccgagctcg
     1801 tcgtcggcct cgacgtccac ccggtgggag aggtcgccgc cgctgatccg ccgcgtcccc
     1861 tcggccagcg cctggatggg gatggtcacc cgccgcgcca gatagagccc cacccaggag
     1921 gaggcgagga ggatcagcag ggtcaccatc aggaagatga ggaggtggct cgcccgcagg
     1981 tcctccttct gtacctcgat ctggcgatag gtctggaagt cctcgaccag ccgctcggtg
     2041 cgctcggcga gcgccgggtc gaggagcgaa ccggcgacca ccaccacgat gggcggcggc
     2101 tcctcctcga tggtgggctc ccggggtgag gcggccgccg cgacggcggc gagcacgagc
     2161 tgtccctggc cgccgggggg cggttggacg cgcgtcgcct ccccctcctc ggcggcctcc
     2221 tcgaggaagt cgcggccggg ctccggcagg tcggggatgc ccgagcgcgg gttgagcacc
     2281 gcctggacga agtcggtgcc gtcgtagacc gccagcacgt cgaggccgag ctcggtctgc
     2341 agctcctgga ggcggcggtt caggctcggg cgctgctcca cctcgcgcag gtcgaacccc
     2401 tcgatcgccg cgaggacccg gccggcatcc cgcaggtggg tgcgctcgat ctggtcgtag
     2461 agcgcctggg ccacggcgtt gccccgctcc agcaccggcc gcaccggcgt gttgaaccag
     2521 cggtcgatgg atccctggag cagctcggtg gcgatggcga agaggaggag gacggggatc
     2581 agcgacaggc cgacataggt cgccaccagc ttgaacttga aggtggagcc gaggatgcgg
     2641 tgacggcgct cgaggagcag cttgacgacg ttgcgcagca gaacgaagag gatggtgagg
     2701 atcaggacga cgttggtgta ccagaggacg aagaggagca cccggttggt caccaggtag
     2761 gaagggaggt cgcgcccctg ctgcgtccag tagaaggtgc cggtgaagag gaacagcagg
     2821 accaccaggg ccccgatgat cagccggttg ttcttgcggt ggcgggcgag cttcgcgcga
     2881 aggtccatgg cgccgcctca gggctgctgg cggggagggt accggaactt gttggagtcc
     2941 gcccaatcgg tggcgatggt caccgggatg aacgagaaga gcaggcgcga gcccatctcg
     3001 gcgcgggcct tgagcagcag ccgccgccgg ctcggcggtg cgtccagggt gaagatcggg
     3061 atgcgctcga gctgcgtcat ggcggcctcg agcgcctcga ggtcgcgcac cgtccggctc
     3121 tccaccagct cgccatcgag ccgcgagtgg acggtgtagg agcgggagac ggcgtcgtac
     3181 agcgcgtcga cctccagggt ggaggcgctc agccggttgt cgtaccagcg cttgcggtcg
     3241 ctgtgcagct cgaaccggta ctggatggtg gtgcgcaggc cgctctccag gcgcgcgacc
     3301 atgcggcggt cgaaggcgtc gcggagcttg aggctggcga gcacccggtt gccgtcgacc
     3361 gcgacctgga ggccggtgat cctggcgtcg gctccggccc gaccggccag gaacgcgagg
     3421 cagaggaacg cgaggaccgc tctcatcggt gccgcgagtg tagcaccgag cgggaacctc
     3481 gcccctcccg cagtggccgg ttccggctac gtcgccgcgg cgggccggct caggtgcgcc
     3541 gtccgcgtcc gcggatcatg cgccagatcg ccaggaggat gaacgcgccc aggatggcga
     3601 tgaagatgct ccgcagatcg aagccggaga tccctccgaa tcccagggcc gtggccagga
     3661 accctcccag gagggcaccc acgatgccga tgatgaccgt gatgacacac ccgcccgggt
     3721 ccttgcccgg catgagcgcc ttggcgatca acccggcgat gaggcccaga acgacccacg
     3781 cgatcaagcc cattcccgct tccatgccta tctcctcccc gccgcggacg gcggatgatg
     3841 actgcgtcta tcccgtaaag ggttgcgagg ctcgtgccag ggcccgccgc cccgggaaga
     3901 ccccggcggc atagaatggc ctcgccatga gcgagacccg cccccgcctc tacctgatcg
     3961 acggttactc caatatcttt cgtgcctact acgccatccg caacctgtcc aactcgcggg
     4021 gggagcccac caacgcggtc tacggcttcg tgaccatgct ccgcaagctg ctgcgcgagg
     4081 acaagcccga cctcatcggc gtcgccctgg acgtcggcaa gacgttccgc agcgagaagt
     4141 tcgaggacta caaggcgaac cgcgccccga tgccggagga cctcgcgagc cagatgccgc
     4201 tggtccgccg cgccatcgag gcgcaccgca tcccaatcct ggagctccag ggatacgagg
     4261 ccgacgatgt cctcggggcc ctcgcctgca aggccgccga cgcggggtat cacgtggtca
     4321 tggtctcggc ggacaaggac ctgatgcagc tcgtctcgcc ccacatctcc ctcttccaca
     4381 ccggaaggaa caagatgtac ggcccgcagg aggtgaccga ggacttcggg ctgccgccgg
     4441 agaaggtggc ggacgtgctc gccctgatgg gcgactcggt cgacaacatc ccgggcgtgc
     4501 cggggatcgg cgagaagggg gccaagtcgc tgatccagga gtacgggtcg ctcgaggagc
     4561 tgctcgcccg cgccggcgag gtgtcgcgca aggcctaccg cgaggggctg cagcagcacg
     4621 cggagcaggc gcgcctctcc aaggagctgt cgaccatcca ctgcgacctg ccgatcccgt
     4681 tcgatcccga ggccctgcgc cacgatccgc cggacaacga cgccctgcgg cagctctaca
     4741 ccgaactgga gttcttctcc ctggtcgagg agctcaagcg cgagccggcc gccgacgcgg
     4801 cggagcgggt tccccccgcc accgagctgg cctcggcggc cgcgtggcac cggcagaccg
     4861 ccgccctcgg tcaggaggac ggcgaggtct tcgtcgccgt gctgggggag gagcgcccgc
     4921 tgggtctggc ggtggggggg ccggagaccg acgaggtgct ctacgccgat ttccggcggg
     4981 gtgatgacct ccgcgcggcg gcgctcgcga ctctcggccg ctgggtcgac aaccccacgg
     5041 tgcgcctctc cggccacaac ctcaaggagg tgctgcgcct gt
//
LOCUS       DASPJI010000576         5032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_720113, whole genome shotgun sequence.
ACCESSION   DASPJI010000576 DASPJI010000000
VERSION     DASPJI010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720113"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VEL74_16120"
     CDS             complement(<1..878)
                     /locus_tag="VEL74_16120"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HYG64102.1"
                     /translation="MKRRIEGARLPNILMGTGLCLTLALGASPALAQTAQQAQGTQAK
                     PTAQPARPSFPATVEGARQFLDEAQKRLMELTTEAGRAQWVQANFITYDTELLAAARN
                     EVLLGESVRYAQEATRFDKVQLPADLRRQMDLLKRSLVLPAPSDPAKTGELTRLTASL
                     ESQYGSGKYCPQGGECMDLEKLSDIIETSRDPKELLDVWTGWRTVSPPMRDEYQRMVE
                     IANEGARELGYKDVGALWRSKYDMPPDQFSAELDRLWGQVKPLYDSLHCHVRAKLNEK
                     YGNEVVPPGEPIPAHLL"
     gene            complement(946..2115)
                     /locus_tag="VEL74_16125"
     CDS             complement(946..2115)
                     /locus_tag="VEL74_16125"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HYG64103.1"
                     /translation="MKLSDRALRIAESATLQVARRALELKARGVSLADFGAGEPDFPS
                     PSVAVEAARQALTEGFTRYTPGSGTPELRAALAESFGRRHGAPWKAAQAVITVGAKAA
                     LFEVALALFEAGQEVVLPSPCWVTFPEQIAFTGAKPVLVPTSLEDGFRIHAGPLLDAV
                     TPRTRAILVNSPSNPTGGIVTAEDLRQIVTFAAERGLVVISDETYERFVYTGAHASAA
                     SLAAEFPETVILIGSFSKTYAMTGWRVGYVLGPREAIQAVEKIQSHATSNPTSFAMVG
                     TLEALEKAEPDVEGMIAEYRVRRDMLVERLNRLPGFECRPPDGAFYAFPHVASCFRPG
                     RQGSVALSEFLLEEARVAVVPGAAFGADDHIRLSFACSRSTLADGLDRMAAVLAD"
     gene            2255..4033
                     /locus_tag="VEL74_16130"
     CDS             2255..4033
                     /locus_tag="VEL74_16130"
                     /inference="COORDINATES: protein motif:HMM:NF025268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HYG64104.1"
                     /translation="MKRLRTLHASCAPALLALALLAPLTGIAGGVAEAQIPRPEKAGV
                     ALHTDRTAYEAGSKAKIAALVTIEPGWPDEKVQYPAHEMQTFAFAEQPLAVYDGEVEI
                     LAEIQVPKGTPAGEIPLQASLRYQACDDSSCLPPVTTQARVTLTVGAAGEETESAATF
                     STGGDAGGTGGAGDSLFLMLCLGLVGGLILNAMPCVLPVLSLKIFGLVRSAGHGRREV
                     VRGTLATSAGILVSFWALAAAAVAARTAGAAVGWGVQFQHPGFVAFLAVIVVLFCLNL
                     WGLFEIQLPWRVAQLGGSGAAREGAAGHFASGLFATLMATPCSAPFLGPALGFALSQE
                     ALQIFAMFTAVGLGMSLPYLVIAAAPGLARFLPKPGAWMETVRGFMGFLLAGAAVWLF
                     YVLAAQVSPERVAGIKLALLALALFTWLRHRSLTGKAFRHAVAAAMVATVAVTLLLAH
                     GGRAGATATTAAVAQPETPGLIAWEPFDRARAESLAAAGTMVFVDVTADWCFTCKFNE
                     KLVLNTPEVAEAFEEHRVVPMKADWTNRDDRITQFLAEHGRYGIPFYLLYLPGGKPHV
                     FGEIPSKDGLVEVVRNGARPDQVAAK"
     gene            complement(4542..>5032)
                     /locus_tag="VEL74_16135"
     CDS             complement(4542..>5032)
                     /locus_tag="VEL74_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009232607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HYG64105.1"
                     /translation="EDPLYVARRMIRFASEDVGLADPQALPQALAAWESYHRLGSPEG
                     ELALAQAVVYLALAPKSVAVYEGYKAARRTVEERPADPVPKVIRNAPTGLMKDLGYGK
                     GYVYAPHTEEGVGGLDCLPEALQGTRFYEPKGKGFEAKLKERLEHFRGLRQEVKEKRG
                     EQ"
BASE COUNT          660 a         1780 c         1809 g          783 t
ORIGIN      
        1 aggaggtggg ccgggatcgg ctcgccgggc ggcacgacct cgttgccgta cttctcgttg
       61 agcttggcgc gcacgtggca gtggagggag tcgtagagcg gcttgacctg gccccagagc
      121 cggtcgagct cggccgagaa ctggtcgggc ggcatgtcgt acttggagcg ccagagggcg
      181 ccgacgtcct tgtagcccag ctcccgcgcc ccttcgttgg cgatctccac catgcgctgg
      241 tactcgtcgc gcatgggggg ggagacggta cgccagccgg tccagacgtc gagcagctcc
      301 ttgggatcgc ggctcgtctc gatgatgtcg gagagcttct cgaggtccat gcactccccg
      361 ccctgcgggc agtacttgcc ggaaccgtac tggctctcga ggctggcggt gaggcgggtc
      421 agctccccgg tcttcgcggg atcgctgggg gcggggagca ccaggctccg cttgagcagg
      481 tccatctgcc gccgcaggtc ggcgggaagc tggaccttgt cgaaccgggt ggcctcctgg
      541 gcatagcgga ccgattcgcc gaggagcacc tcgttgcgcg cggccgccag gagctcggtg
      601 tcgtaggtga tgaagttggc ctgcacccac tgggcgcggc cggcttcggt ggtgagctcc
      661 atcaggcgct tctgggcctc gtccaggaac tggcgggccc cctcgaccgt cgccggaaag
      721 gacggccggg ccggctgggc cgtgggcttc gcctgggtcc cctgggcctg ctgggcggtc
      781 tgggccagcg ccggggaggc cccgagcgcc agcgtcaggc agagcccggt ccccatcaga
      841 atattgggca gccgggcccc ttcaatcctt cgcttcataa aatgcctcca cagtaggtct
      901 gatgtacggc ccgcgggccg caagcgagtc agtatacccg ccggctcaat cggcgagcac
      961 ggcggccatc cggtccagcc cgtcggccag ggtggaccgg gagcaggcaa aggagagccg
     1021 gatgtggtcg tcggcgccga aggcggcgcc gggcaccacc gccacccgcg cctcttcgag
     1081 gagaaactcg gagagggcga cggagccctg gcgcccgggc cggaagcagg aggccacgtg
     1141 cgggaaggcg tagaaggcgc cgtccggcgg ccggcactcg aagccgggga ggcggttgag
     1201 ccgctccacc agcatgtcgc gccgcacccg gtattcggcg atcatcccct cgacgtccgg
     1261 ctcggccttc tccagcgcct ccagggtccc caccatggcg aacgaggtgg ggttggaggt
     1321 ggcgtggctc tggatcttct ccaccgcctg gatggcctcc cgggggccga gcacgtagcc
     1381 gacgcgccag ccggtcatcg cgtaggtctt ggagaacgag ccgatgagga tcacggtctc
     1441 cgggaactcg gcggcgaggc tggcggcgct ggcatgggcg ccggtgtaga cgaagcgctc
     1501 ataggtttcg tcggagatca ccacgagccc ccgctcggcg gcgaaggtga cgatctgccg
     1561 cagatcctcg gcggtcacga tcccccccgt cgggttggac ggcgagttga ccaggatggc
     1621 gcgggtgcgc ggggtcaccg cgtcgaggag cggcccggcg tggatgcgga agccgtcctc
     1681 cagggaggtc ggcaccagca ccggcttggc gccggtgaag gcgatctgct ccgggaaggt
     1741 gacccagcag ggggagggga ggaccacctc ctgcccggcc tcaaacaggg cgagcgccac
     1801 ctcgaacagg gcggccttgg cgccgacggt gatcaccgcc tgcgccgcct tccagggggc
     1861 gccgtggcgc cggccgaagg actcggcgag cgcggcccgc agctcgggcg tgccgctgcc
     1921 cggcgtgtag cgggtgaagc cttcggtgag cgcctggcgg gcggcctcca ccgccaccga
     1981 gggtgacggg aagtccggct caccggcgcc gaagtcggcc agcgatacac ccctggcctt
     2041 gagctccagg gcgcggcgcg cgacctggag cgtcgcggat tcagcgatgc ggagggcccg
     2101 gtctgagagc ttcacggcgg tgcatcctat catcacccgc cgccgccttc ccgcgtggta
     2161 ccctcccgcg gcggatcggc gaataaacgg ccgctccggt ctgttcaccg ggttggaaga
     2221 ctcacttgtc cgttcgggac gaggtggcca tctcatgaag agactacgca ccctgcacgc
     2281 gagctgtgcc ccagccttgc tggcgctggc cctcctggcc cccctgaccg gtatcgccgg
     2341 cggcgtggcc gaggcccaga tcccccggcc cgagaaggcc ggggtcgccc tccataccga
     2401 ccgcaccgcc tacgaggcgg gctcgaaggc caagatcgcc gccctggtga cgatcgagcc
     2461 gggctggccg gacgagaagg tccagtaccc cgcgcacgag atgcagacgt tcgccttcgc
     2521 cgagcagccg ctggcggtct acgacggcga ggtggagatc ctggcggaga tccaggtgcc
     2581 gaaggggact ccggcggggg agatcccgct ccaggcgtcg ctgcgctacc aggcctgcga
     2641 cgactcctcg tgcctgccgc cggtcaccac ccaggcgcgg gtgacgctca cggtcggcgc
     2701 cgcgggcgag gagacggaga gcgcggccac cttctctacc ggcggcgatg ccggcgggac
     2761 gggcggcgcg ggggacagcc tgttcctgat gctgtgcctc gggctcgtcg gcgggctgat
     2821 cctcaacgcc atgccctgcg tcctgccggt gctctcgctc aagatcttcg gcctggtgcg
     2881 gagcgccggg cacggccgcc gggaggtggt gcgcggcacg ctcgccacct cggcgggcat
     2941 cctggtctcc ttctgggcgc tggccgcggc cgccgtggcg gcgcggaccg ccggcgcggc
     3001 ggtgggctgg ggggtgcagt tccagcaccc cggcttcgtc gccttcctcg ccgtgatcgt
     3061 cgtcctcttc tgtctcaacc tctgggggct gttcgagatc cagctcccct ggcgcgtggc
     3121 gcagctcggc ggcagcggcg ccgcccgcga gggcgcggcc ggccacttcg cctccggcct
     3181 gttcgccacc ttgatggcca ccccctgctc ggcgcccttc ctcgggccgg ccctgggctt
     3241 cgccctgtcg caggaggcgc tgcagatctt cgccatgttc accgccgtcg ggctcggcat
     3301 gtcgctcccc tatctggtga tcgcggcggc gccggggctc gcgcgcttcc tgccgaagcc
     3361 gggcgcgtgg atggagacgg tgcgcggctt catgggcttc ctgctcgccg gcgcggcggt
     3421 ctggctgttc tacgtgctgg cggcccaggt gtcgccggag cgggtggcgg ggatcaagct
     3481 cgccctcctg gccctggccc tgttcacctg gctgcgtcac cgcagcctga ccggcaaggc
     3541 cttccggcac gccgtcgccg ccgcgatggt ggcgacggtc gccgtgacgc tgctgctcgc
     3601 ccacggcggc cgtgcggggg cgaccgccac gactgccgca gtggcgcagc cggagacccc
     3661 gggcctgatc gcctgggagc cgttcgaccg cgcccgcgcc gagtcgctgg ccgccgccgg
     3721 caccatggtc ttcgtcgacg tgaccgccga ctggtgcttc acctgcaagt tcaacgagaa
     3781 gctggttctc aacacccccg aggtcgccga ggccttcgag gagcaccggg tggttccgat
     3841 gaaggcggac tggaccaacc gcgacgaccg cattactcaa ttcctcgccg agcacggccg
     3901 ctacggcatc cccttctatc tgctctacct gccgggaggg aagccgcatg tcttcggcga
     3961 gatcccgagc aaggacgggc tggtcgaggt ggtccgcaac ggcgccaggc cggaccaggt
     4021 cgcggccaag taggccctgc cccccagccc cctctctccc cgggagaggg ggaggcgacc
     4081 cgatacgcta tcgaagtctc cggcgcgccc tcagagtttc cgacgccctc tcagagtttc
     4141 cggcgccttg ccggagctcc cggcgcgctg ccgaagcttc caacgcgctg tcagagcttc
     4201 cggcgtgccg tcggagtctt cggcgcgctg tcgcgatctc tgacgcgccg ccggaaggtt
     4261 tggcaggccg ttgaagcctc cgacgtggcg tcgccatctt cgacaggcca tcgggagctt
     4321 ccaacgcgcc gtcgtagtct ccgacgggcc gtcgtacgct ccggcagacc gtcgtactct
     4381 tcgccaagcc gccgggagct tccaatccgc cgccggagct tccggcgcgc cgccgcaatc
     4441 tccgccaaga gaaaagcgcc tcccccgcgg cggcggagaa ggcgctgcgt ccttctatgg
     4501 atgggcgggc cgcgcgtagg ctccccggcc tctctctacc tttactgttc cccccgtttc
     4561 tccttgacct cctgccggag cccccggaag tgctccagcc tttccttgag cttggcctcg
     4621 aagcccttcc ccttgggctc gtagaagcgg gtcccctgga gcgcttcggg gaggcagtcg
     4681 aggcctccca ccccctcctc ggtgtggggg gcgtagacgt agcccttgcc gtacccgaga
     4741 tccttcatca gcccggtcgg cgcgttgcgg atcaccttgg ggaccggatc ggccggccgc
     4801 tcctcgacgg tgcgccgggc cgccttgtag ccctcgtaga cggcgacgct cttcggcgcg
     4861 agcgccaggt agacgacggc ctgggcgagc gccagctcac cctcgggcga gccgagacgg
     4921 tgataggact cccaggcggc cagggcctgc ggcagcgcct gcgggtcggc gagccccacg
     4981 tcctcgctgg cgaagcggat catgcggcgg gcgacgtaga gggggtcctc cc
//
LOCUS       DASPJI010000577         4876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_784037, whole genome shotgun sequence.
ACCESSION   DASPJI010000577 DASPJI010000000
VERSION     DASPJI010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784037"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VEL74_16140"
     CDS             <1..642
                     /locus_tag="VEL74_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64106.1"
                     /translation="GGTVATLTLLGTRPEKGRWLRRGDAAPGHALWLGGTVGESAAGA
                     LLAGRGARLTGSRVDLPPDLGDSGGLAAAARRAVRRHLLPEPQLELGHWLGEQREGAA
                     MDLSDGLARDLHRLCRESGTGAEITFETLPLPDRFIPLCERIGADPRALALSGGEDYI
                     LLFTLPESVEPPSRFGCRRIGTINGRGRRIVLVDQGAARDLPDTGWDHLSKMK"
     gene            complement(703..1563)
                     /locus_tag="VEL74_16145"
     CDS             complement(703..1563)
                     /locus_tag="VEL74_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64107.1"
                     /translation="MEPDTTQRGEEQSGSDRLYTLSEVSTKTGISMPTLQRYKKTYQS
                     RIPAVGKGRKQRYPESALPVFEELKNENAGRRGRPRKDASVAPARRGAAKSAPAKAAG
                     GGRRAAASKRPAAAPAKRGRGAAKATATQGTGARRGRPPGSKNAQKAGAQKGSRPGNL
                     MTLTQVSEQTGISYPTLVRYVRLHSDRLPSEGTGRARRFYPEAVSVFRELRQESGRGG
                     RKAGSGRAAAGAGRPGRPASRGRAAGAAGRDEGLSGMLQQRLKALEKSQENLEKRFRG
                     FVSSLQKLFR"
     gene            1597..2277
                     /locus_tag="VEL74_16150"
     CDS             1597..2277
                     /locus_tag="VEL74_16150"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYG64108.1"
                     /translation="MKNKLPNAVIIKNKSRSVNRPVKVRTSRGTPSRVAWSFRAFHEP
                     LKTASLAYNRAMDSQSKRDLYREIEQEVAAVVEGLEDPIAAMATCACIIYERVPGCSW
                     AGFYRVIAPRLLRVGPYQGPLGCLDIPFDRGVCGAAARERRTQVVPDVHAFPGHIACD
                     STARSEIVVPVYDPDGELIAVLDLDSREPATFDEVDQEGLESIVQRLRPAMKQGTGAP
                     VNAHGAAH"
     gene            complement(2315..>4876)
                     /locus_tag="VEL74_16155"
     CDS             complement(2315..>4876)
                     /locus_tag="VEL74_16155"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYG64109.1"
                     /translation="EEERVALARTLVCLGRFAGALAALEGTVSVASGMVRARCQLLLG
                     QLRAARATLRRLELTALAPGEVIEMADIAARVYANSGDAGAAAVWVRRALDCDPGSRV
                     AIQARLVAAAAAWDRRDRSLMDAYLAEAEGALAEPGLAWRWHHLRGLRAELEADGSAM
                     VEHLSRALRASRRLLARHEAAGLWNDLGLGRAQAGDLAGAERAFLHVLRLYGGCDGPR
                     RATLALHNLAEIRLRRGRFAGVREILESSTAENRLSGNLRGLAQDLELWARFELALGR
                     SQAALALCREAGREMESREVSWRREETAVLAARALGWLREVEPAATELAAVTQEAVAG
                     QLEPEERPALWAHAGLVEAALEAAAPTPFSSFWQALLSGESVPVPAWQVLDRLEPYRA
                     ARLVFDAETLRPGVAPPHQVRSAAAFLRSVGAVPLAERLEARDGGPWEALATYLAGSG
                     GPEAASALLAGAGCPEAELVWMTPGGERRVLCSGAGGEHELTAEAGEGSLVLRAPRMA
                     PILRALFALLVRDLTGKVGAPATLRTEEPPVRPVPTGGIIGESPPLKAALERLARLAA
                     GELPILILGESGTGKELAARHVHRLSPRAKGPFVPVNSAALSETLLLSDLFGHARGAF
                     TGADRDRAGVFETAHGGTVFLDEIGDLPPVAQGMLLRVLQEGEVRRLGESLPRKVDAR
                     VLTATHRDLSKMVREGTFRQDLFYRLKVGSLEMPPLRDRGEDVLLLAEQILGRLRLPR
                     PQPAMRLSREARARLLEYRWPGNVRELQNVLSVAATLADEGVIGPPHLELPDAGSHGS
                     EGTDLAAAYHRELDAVRRRLIEEALEACGGNVAEAARRLGMSRQGLSYIVRTLRR"
BASE COUNT          745 a         1775 c         1642 g          714 t
ORIGIN      
        1 ggcggaacgg tggcgacgct caccctgctg ggcacccggc ccgagaaggg gcgctggctc
       61 cgccgcggcg acgccgctcc ggggcatgcg ctctggcttg gaggcaccgt cggagagtcc
      121 gccgccggcg ccctgctggc cggccggggg gcgcgcctga ccggctcccg ggtcgacctc
      181 cctccggacc tcggtgacag cggagggctc gccgctgccg cccgccgcgc cgtgcgccgg
      241 cacctccttc ccgagcccca gctcgagctc ggtcattggc ttggtgaaca gagggaaggg
      301 gccgcgatgg atctatcgga cggcttggcc cgcgacctcc accggctctg ccgcgagagt
      361 ggaacgggcg ccgagatcac gttcgaaacc ctgcccctgc ctgatcgatt catccctcta
      421 tgtgaacgga tcggagccga cccgcgggcg ctggcgctga gcggaggcga ggactacatc
      481 ctgttgttca cattgcctga aagtgtagag ccgccgtcgc gcttcggctg ccggaggatc
      541 ggaacgatca acggccgcgg ccggcgcatc gtcctcgtcg atcagggagc ggctcgcgat
      601 ctaccggaca ccggctggga tcacctgtcg aaaatgaaat gaaaacgaaa ggccccggct
      661 cgccagagcc ggggccttgt ggattcgtcg tagcgctaca actcagcgga acagcttctg
      721 aaggctggac acgaagccgc ggaacctctt ctcgaggttc tcctgcgact tttcgagagc
      781 cttgagccgc tgctgcagca tgccgctcag gccctcgtca cgaccggcag cgcctgccgc
      841 gcggccacgg ctggcaggac gtcccgggcg cccggcgccg gcggcagccc ggcccgaacc
      901 cgccttgcgg ccaccgcggc cgctctcctg ccgcagctca cggaagacac tgaccgcctc
      961 gggatagaaa cgccgcgcgc ggccggtgcc ctcgctgggc agacgatcgg agtggagacg
     1021 gacatagcgc accagagtgg gataagaaat gccggtctgc tcactcacct gcgtgagcgt
     1081 catcagattg cccggccgcg agcccttctg agcgcccgcc ttttgggcgt tcttgcttcc
     1141 cggcggacgg ccgcgccgcg cgccggtgcc ctgagtcgcg gtggccttgg cggccccacg
     1201 gccgcgcttc gccggcgcgg ccgcggggcg cttcgaagcc gccgcgcgac ggccgccgcc
     1261 ggccgccttg gccggagcgc tcttcgcggc gccacgccgc gccggagcca ccgaagcgtc
     1321 cttgcgcggc cgcccgcgcc ggcccgcgtt ctcgttcttg agctcttcga aaaccggaag
     1381 tgcgctctcg gggtagcgct gcttgcggcc cttccccacg gcggggatgc ggctctgata
     1441 cgtcttcttg tagcgctgca gggtcggcat cgagatgccg gtcttcgtcg aaacttccga
     1501 aagggtgtag agccggtcgc tgcccgactg ctcctcacca cgttgtgtcg tatctggttc
     1561 catgtttccc ctagcctcct gtagtcttga atttgaatga agaacaagct cccgaacgca
     1621 gtcattatca aaaacaagtc gcgcagtgtc aatagaccag tgaaagttcg tacgagtcgt
     1681 ggcactcctt cgcgcgtcgc gtggagcttt cgagcgtttc acgagccatt gaaaaccgcg
     1741 tctctcgcgt acaatcgagc gatggacagc cagagcaaac gagatctcta ccgtgaaatc
     1801 gagcaggagg tcgccgccgt cgtcgaaggg ctcgaagacc ccatcgccgc catggcgacc
     1861 tgcgcctgca tcatctacga gagggttccg ggctgcagtt gggccggttt ctatcgcgtg
     1921 atcgctcccc gcctgctgcg cgtcggccct tatcaaggcc ctctgggatg cctcgatatt
     1981 ccctttgacc ggggcgtctg cggcgccgcc gcgcgcgagc gccggacgca ggtcgtgccg
     2041 gacgtgcacg ctttcccggg ccacattgcc tgtgattcca ccgcgcgatc ggagatcgtc
     2101 gtcccggtct acgatccgga cggtgagttg atcgccgtcc tcgatctcga ctcccgtgag
     2161 cccgcgactt tcgacgaggt ggatcaggaa ggcctggaat ctatcgtcca gcgcctgcgg
     2221 ccggcgatga aacagggtac cggagcaccg gtcaacgctc acggcgccgc gcattgacct
     2281 ccggccggcg gcggcttcca agcgtttact tcttctagcg gcgaagagtg cggacgatat
     2341 aggagaggcc ctggcgcgac atcccgaggc ggcgcgccgc ctcggcgaca ttgccgccac
     2401 aggcttccag ggcctcttcg atcaaacgcc ggcgcaccgc gtccaactcg cggtgatagg
     2461 ccgccgcaag atcggtcccc tcgcttccat ggctcccggc atcgggcagc tccaggtgcg
     2521 gcggaccgat cacaccttcg tccgccagcg tggccgcgac gctcagaacg ttctgcagct
     2581 cacggacatt gccaggccag cgatattcga gcaggcgtgc gcgggcctcg cgagacaatc
     2641 tcatggccgg ctgaggacgt ggcagacgca ggcgtcccag gatctgctcg gcgaggagca
     2701 acacgtcctc gccgcgatcg cgcagcggcg gcatctcgag gcttcccacc ttcaagcgat
     2761 agaaaaggtc ctgccggaag gtcccctccc gcaccatctt cgacagatca cgatgcgtgg
     2821 ccgtcagcac ccgtgcgtcg accttgcgtg gcaggctctc gcccagccgc cgcacctctc
     2881 cttcctgtaa tactcgcagc aacattccct gcgccaccgg cggcaggtct ccgatctcgt
     2941 cgaggaagac ggtgccgccg tgggcggttt cgaaaacacc ggcgcgatca cggtccgccc
     3001 cggtgaacgc gccgcgggcg tgaccgaaca gatcggagag gaggagggtt tcggaaagcg
     3061 ccgcggagtt gaccggaacg aacggtccct tggcacgcgg gctgaggcga tggacgtgac
     3121 gcgccgccag ctccttgccc gtgccgctct cacccaggat cagaatgggg agctccccgg
     3181 ccgccagacg ggcgagccgc tccagcgccg ccttgagcgg cgggctctcg ccgatgatcc
     3241 cgccggtcgg caccggccgg accggaggct cctcggtcct cagcgtggcc ggcgcgccga
     3301 ctttgccggt gagatcacgg accagaaggg cgaacagcgc tctcaggatg ggggccatcc
     3361 gcggcgcccg caacaccagc gagccctcgc cggcctcggc cgtcagctcg tgctcgcccc
     3421 ccgctcccga gcagagcacc cgtcgctctc ctccgggcgt catccagacc agctcagcct
     3481 cggggcaacc ggccccggcg aggagagccg acgcggcctc gggcccgccg ctaccggcga
     3541 gataggtggc caacgcctcc cacggcccgc cgtccctggc ctccagacgc tcggccagcg
     3601 ggacggcccc cacgctccgc aggaaggccg ccgcagacct cacctggtgg gggggagcca
     3661 cacccggacg gagcgtctcg gcatcgaaga cgagccgcgc ggcgcgatag ggctcgagcc
     3721 ggtccagcac ttgccaggcg gggacgggca ccgactcccc ggacaggagg gcctgccaga
     3781 aggaggagaa aggggtaggc gccgccgctt cgagggcagc ctcgacaaga cccgcgtggg
     3841 cccatagcgc gggccgctct tcgggctcga gctgcccggc cacggcctcc tgcgtcacgg
     3901 ccgcgagctc ggtggccgcc ggctccacct cccgcagcca gcccagggcg cgggcggcga
     3961 gcactgcggt ctcctcccgg cgccaggaga cctcgcggct ctccatctcc ctcccggcct
     4021 cacggcagag ggccagcgcg gcctgggaac ggccgagcgc gagctcgaag cgtgcccaga
     4081 gctccagatc ctgcgcaagg ccgcggaggt tgcctgaaag gcgattctcg gcggtcgagc
     4141 tctccaggat ctcccggact ccggcgaagc gccccctgcg cagccggatc tctgccaggt
     4201 tgtggagtgc cagggtcgcc ctgcgcgggc cgtcgcagcc gccgtagagg cgcagcacgt
     4261 gcaggaaggc cctctcggcg ccggcgaggt cccctgcctg ggcgcgcccc agacccaggt
     4321 cgttccacag cccggccgcc tcgtggcggg cgagcaggcg ccggctcgcc cggagcgccc
     4381 gcgaaaggtg ctccaccatg gcggagccgt cagcctccag ttcggccctc agcccgcgca
     4441 ggtgatgcca gcgccaagcg aggccgggct ctgccagagc accctcggcc tctgccagat
     4501 aggcgtccat gagcgaacgg tcgcggcggt cccaggcggc cgccgcggcc accagccggg
     4561 cctggatggc gacacggctg ccgggatcgc agtccagggc ccgccgcacc cagacggcgg
     4621 ccgcgccggc gtcgccgctg ttggcataga cgcgggcggc gatgtcggcc atctcgatca
     4681 cctctcccgg ggcgagggcc gtcagctcca ggcgacggag ggtcgcccga gccgcccgga
     4741 gctgtccaag gaggagctgg cagcgggccc tgaccatccc ggaagcgacc gagaccgtcc
     4801 cttccagggc cgccagtgca ccggcgaagc gtccgaggca caccagcgtc cgcgccagcg
     4861 ccacccgctc ctcttc
//
LOCUS       DASPJI010000578         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_37454, whole genome shotgun sequence.
ACCESSION   DASPJI010000578 DASPJI010000000
VERSION     DASPJI010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37454"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VEL74_16160"
     CDS             complement(<1..951)
                     /locus_tag="VEL74_16160"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYG64110.1"
                     /translation="MEPYNVSMLRPDRPERSDGFAEKRRILQLETLYDLALALHAQRP
                     EQELVDELLQRVCAVLDPAAAVAVTRDGYGSPVGVATVGWSESPPGGAALLAAPIWRE
                     LLTEGRLLARAGGELAGRPYRQLLAVPLAYRGVYLGYVALLDKEVRGDLDPSFTPDDR
                     RFLDSVATLAGAALDAARQVERLEAQRERLEEENKALKGRLSGELAGRRIVAHAPPMR
                     RVLEVVERVAPRGVNVLVRGESGTGKELVAKLLHVLSGRDGALIAVNCAALPDSLLES
                     ELFGIEGGVATGVQARPGKFELADRGTLFLDEIGDMLPSLQ"
     gene            complement(1035..1238)
                     /locus_tag="VEL74_16165"
     CDS             complement(1035..1238)
                     /locus_tag="VEL74_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64111.1"
                     /translation="MLRKLFAVAVLSLATLAAVATPRAAFAIEYICSCSVCTAHPGYG
                     CYDMKLSPPFNRVSCSTYLANRC"
     gene            complement(1251..1448)
                     /locus_tag="VEL74_16170"
     CDS             complement(1251..1448)
                     /locus_tag="VEL74_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64112.1"
                     /translation="MLRKLFAVAVLIIAAAAANPAPAPAIDFICNCQVCSDGTGPGCR
                     DLRAGGFRSCSSWWAQYGSSC"
     gene            complement(1553..>2470)
                     /locus_tag="VEL74_16175"
     CDS             complement(1553..>2470)
                     /locus_tag="VEL74_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HYG64113.1"
                     /translation="LVNDILYKALARHFYRATDRPGEHEEILGLDQLDKVIAIDQSPI
                     GRTPRSNPATYTNVFNPVRNLMAMTPEARMRGYQPGRFSFNVPGGRCEACKGDGQIKI
                     EMHFLPDIYVTCEVCGGKRYDRETLAVRYKGLNIAEILDLPVEQAHEMFTNIPAVERI
                     LSTLCEVGLGYIRLGQPATTLSGGEAQRVKLSRELCKRSTGRTLYLLDEPTTGLHFDD
                     VGKLLKLLHRLVELGNTVVVIEHNLDVIKTADWVIDLGPEGGAGGGRLVAVGTPEEVA
                     KVSESYTGRYLKSVLPAPRKAARRAGGRR"
BASE COUNT          368 a          820 c          908 g          374 t
ORIGIN      
        1 ctggagggag ggcagcatgt cgccgatctc gtcgaggaac agggtgccgc ggtcggccag
       61 ctcgaacttg cccgggcgcg cctggacgcc ggtggcgacg cccccctcga tgccgaagag
      121 ctcgctctcg agcagcgaat cggggagggc cgcgcagttg accgcgatca gcgcgccgtc
      181 gcggccggac agcacgtgga ggagcttggc gaccagctcc ttgccggtgc cgctctcgcc
      241 acgcaccagg acgttgacgc cgcgcggcgc cacccgctcc acgacctcga gcacccgccg
      301 catgggcggg gcgtgggcga cgatgcgccg gccggcgagc tcgccggaga gcctcccctt
      361 gagcgccttg ttctcctcct ccaggcgctc gcgctgcgcc tccagccgct ccacctggcg
      421 ggcggcgtcg agggcggcgc cggcgagggt ggcgacggag tcgaggaagc ggcggtcgtc
      481 cggggtgaac gacggatcga ggtcgccccg cacctccttg tcgaggagag ctacgtagcc
      541 gaggtagacg ccgcggtaag ccagggggac ggcgaggagc tggcggtagg ggcggccggc
      601 caactcgccg ccggcgcggg cgaggaggcg cccttcggtg agcagctcgc gccagatggg
      661 cgcggcgagg agcgcggcgc cgccgggggg agactcgctc caccctacgg tcgccacgcc
      721 caccgggctg ccgtagccgt cgcgggtgac cgccacggca gccgccggat cgaggacggc
      781 gcagacccgc tgcagcagct cgtccaccag ctcctgctcc gggcgctggg catggagcgc
      841 cagcgccaga tcgtagaggg tctcgagctg gaggatgcgc cgcttctcgg cgaaaccgtc
      901 ggacctctcc ggccggtccg gtctcagcat ggagacattg tacggttcca tgccgctctc
      961 cgtctcatcg ccaacgagtg ccggtgtccc ccacacggat cgatatacga cgggggagca
     1021 ccggctgttt cacgtcagca gcggttggcg aggtaggtgc tgcaggagac cctgttgaac
     1081 gggggactga gcttcatgtc atagcagccg tagcccgggt gcgccgtgca gacgctgcaa
     1141 ctgcagatgt actcgatggc gaaggcggcc ctgggagtgg cgacggcggc cagcgtggcg
     1201 agggaaagga cggcgacggc gaagagcttc ctcagcatgg actttctcct ttaacaagag
     1261 ctgccgtatt gggcccacca gctggaacag gacctgaaac cgccggcccg caggtcccgg
     1321 cagccggggc cggtgccgtc cgagcagacc tggcagttac agatgaagtc gatggcgggg
     1381 gccggcgcgg ggttggcggc cgcggcggcg atgatgagga cggcgacggc gaacagcttc
     1441 ctgagcatcg gattcctcca ggttacgagg aggaaccgcg cccacctccc ctcccggtcc
     1501 cgcctctatc tggttcggtg tccgggaggg gtgagacggg acagaatctg cttcagcgcc
     1561 gtccaccggc gcgccgtgcc gccttgcggg gggccggcag cacgctcttg agataccggc
     1621 cggtgtagct ctcggagacc ttcgccacct cctcgggggt gcccacggcg accagccggc
     1681 cgccccccgc cccaccctcg ggcccgaggt cgatcaccca gtccgcggtc ttgatgacgt
     1741 cgaggttgtg ctcgatgacg acgacggtgt tgcccagctc caccagacga tgcaagagct
     1801 tcagcagctt gccgacgtcg tcgaagtgga ggccggtggt cggctcgtcc agcaggtaga
     1861 gggtgcgccc ggtggagcgc ttgcagagtt cgcgggagag cttgacccgc tgcgcctcgc
     1921 cgcccgacag ggtggtcgcc ggctgcccca ggcggatgta gcccagccct acctcacaaa
     1981 gggtggagag gatccgctcg acggccggga tgttggtgaa catctcgtgc gcctgctcca
     2041 ccggcaggtc caggatctcg gcgatgttca gccccttgta acgcaccgcc agggtctcgc
     2101 ggtcgtagcg cttgccgccg cagacctcgc aggtgacgta gatgtcgggc aggaagtgca
     2161 tctcgatctt gatctgcccg tcccccttgc acgcctcgca gcgcccgccc gggacgttga
     2221 agctgaaccg ccccggttgg tagccgcgca tccgcgcctc cggggtcatg gccatgaggt
     2281 tgcggacggg gttgaagacg ttggtatagg tcgccgggtt cgaccgcggc gtgcggccga
     2341 tggggctctg gtcgatggcg atgaccttgt cgagctggtc gaggcccagg atctcctcgt
     2401 gctcgccggg ccggtcggtg gcgcggtaga agtggcgcgc cagggccttg tagaggatgt
     2461 cgttgaccag
//
LOCUS       DASPJI010000579         3878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_550372, whole genome shotgun sequence.
ACCESSION   DASPJI010000579 DASPJI010000000
VERSION     DASPJI010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550372"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1839)
                     /locus_tag="VEL74_16180"
     CDS             complement(<1..1839)
                     /locus_tag="VEL74_16180"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG64114.1"
                     /translation="MVEISDRLAGLSAERRALLEALLRQRAAATAPRPRVETIPAQPR
                     PAGSFPLSFSQERLWFLAQMDPGGAGYNIPLVLDFLGPLEPPALEASLARVVARHEML
                     RTRFDFAEEGAEDGAGGRPVQIVEPAAGFLLPVVDLAGLPAEVRAGEARRLAMAEARR
                     PFDLAAGRLLRAQLLRLAAKEHRLVANLHHIVSDGWSTSILAREVGQVYRAWLAGATP
                     DLPALPIQYADFAVWQRQTLTGGALEAQVEHWRRTLAGLPPVLELPSDRPRPPVRTPR
                     GGTLGFVLSPLGSERLRELGRGGDGGTLFITLLSGLFSLLQRYTERDDLCVGSPVAGR
                     QQVETEGLIGFFVNTLVLRADLSGDPPFRQALARVREVVFDAHAHQDVPFERLVAELS
                     PERSLDHSPLFQVLFTFQNVPSAALDLGGLRIAPVEVETGTTKFDLGLSMAEEPAGVW
                     GVLEYSLDLFEPSTIARMAGHLRALLEGAAAHPGRRLSELPMLGPSEAQQLLAEWNDT
                     EALYPGRELALHELVFEQAERTPEAVAVEQGEEHLTHAELRRRAGWLAYRLRRLGVAP
                     EVPVALCAERSPEMVVGLLGILEAGGAYVPLDPDYPAERLAYMVEDA"
     gene            2153..2515
                     /locus_tag="VEL74_16185"
     CDS             2153..2515
                     /locus_tag="VEL74_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017307781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH domain protein"
                     /protein_id="HYG64115.1"
                     /translation="MSDLVQRLGTEQDVEVSMRPETTVEYFKPAVDRGYVHIKFIHTR
                     GGTELGIRLDRDASDFSQADWENKTGKVKIVGNLILDYVKVRFHGTIDLSTLKGTGYL
                     ENLGEYKPWEEKAAAEAT"
     gene            complement(2512..3441)
                     /locus_tag="VEL74_16190"
     CDS             complement(2512..3441)
                     /locus_tag="VEL74_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HYG64116.1"
                     /translation="MVNFERVWARLTGKVRSWIEALLLHLPNLVVAVLVLVGAWLVAR
                     AVRSLVHRFLHRVSHSDQVNRLVSQAVYIGIVAAGLFAALGILGLQKTVASLLAGAGI
                     VGLALGFAFQDIAANLMAGIYLSVKRPFRHGHLIKTQDYFGTVERIHLRWTEMRTQQG
                     QLVLIPNKQVFENPLMNYTTTGRRRVDLSVGVSYGDDLARVREVAIRALEEVSNRTPD
                     RPVELFYEELGESSINFVVRFWIPFSQQRDYMAARSEAIERLKRAFDAEGITIPFPTR
                     TLDFAVKGGERLSEALAEAGIPELAGSRPRSGR"
     gene            complement(3483..>3878)
                     /locus_tag="VEL74_16195"
     CDS             complement(3483..>3878)
                     /locus_tag="VEL74_16195"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HYG64117.1"
                     /translation="ASLDFVLRLRETGEPVGCSAYLEIRPAHRGLEIGRTWIARRWQG
                     TRVNPESKYVLLRHAFEALGAVRVQFKTDFNNLHSQRAIEKLGARREGVLRRYQMRAN
                     GSFRDTVMYSITDGEWPEVRAGLEARLAR"
BASE COUNT          630 a         1405 c         1344 g          499 t
ORIGIN      
        1 cgcgtcctcc accatgtagg ccaggcgctc ggccggatag tcggggtcca gggggacgta
       61 cgcgccgccc gcctcgagga tgcccagcag gcccaccacc atctccggcg agcgctcggc
      121 gcagagcgcc accggcacct ccggcgcgac cccgaggcgc cgcagccggt aggccagcca
      181 gcccgcccgg cggcgcagct cggcgtgagt caggtgctcc tccccctgct cgaccgccac
      241 cgcctccggt gtgcgctccg cctgctcgaa gaccagctcg tgcagggcca gctcccgccc
      301 ggggtagagc gcctccgtgt cgttccactc cgccagcagt tgctgggcct cggacggacc
      361 gagcatcggc agctccgaca ggcggcggcc gggatgggcg gccgcccctt cgagcagcgc
      421 ccgcaggtgg ccggccatgc gcgcgatggt ggagggctcg aacaggtcga ggctgtactc
      481 caggacgccc cagacccccg ccggctcctc cgccatggac aggccgaggt cgaacttggt
      541 cgtccccgtc tccacctcga ccggcgcgat gcgcagcccc cccaggtcga gcgcggcgga
      601 gggcacgttc tggaaggtga acagcacctg gaacagcggc gagtgatcca ggctccgctc
      661 cggcgacagc tccgccacca ggcgctcgaa cggcacgtcc tggtgggcgt gggcatcgaa
      721 caccacctcg cgcacccgcg ccagcgcctg ccggaacggc gggtcgcccg acaggtcggc
      781 gcgcagcacc agggtgttga cgaaaaagcc gatgagcccc tccgtctcca cctgctgccg
      841 gccggcgacg ggcgagccga cacacaggtc gtcgcgctcc gtgtagcgct gcaacagcga
      901 gaagaggccc gacagcaggg tgatgaacag cgtcccgccg tcgccgccgc ggcccagctc
      961 ccgcagcctc tccgagccca ggggggagag gacgaagccc agagtgccgc cccgcggcgt
     1021 gcgcacgggg gggcgcggcc ggtcggacgg cagctccagc accggcggca gcccggccag
     1081 cgtccgccgc cagtgctcca cctgggcctc cagcgcgcct cccgtcagcg tctggcgctg
     1141 ccagaccgcg aagtcggcgt actggatcgg tagcgccggc agatccggcg tcgcgccggc
     1201 gagccacgcc cggtagacct gccccacctc ccgcgccagg atgctggtcg accagccgtc
     1261 gctgacgatg tggtgcaggt tggcgacgag ccggtgctcc ttcgccgcca gccgcagcaa
     1321 ctgcgcgcgc agcagccgcc cggccgccag atcgaagggc cggcgggcct ccgccatggc
     1381 gaggcgccgc gcctccccgg cgcgcacctc cgccggcagg ccggccaggt ccaccacggg
     1441 cagcaggaag ccggccgccg gctcgacgat ctgaaccggc cggcctccgg cgccgtcctc
     1501 cgcgccctcc tcagcgaagt cgaagcgggt gcgcagcatc tcgtggcggg ccaccacccg
     1561 ggccaggctc gcctccaggg ccggcggctc cagcggtccc aggaagtcca ggaccagggg
     1621 gatgttgtag ccggcgccgc cggggtccat ctgggccagg aaccagagac gctcctggga
     1681 gaaggagagc gggaacgagc ccgccggccg gggctgcgca gggatcgtct ccacccgcgg
     1741 acggggggcc gtggcggccg cgcgctgccg cagcagggcc tccagcagcg cccgccgctc
     1801 cgcggacagg cccgccagac gatcggagat ctcaaccatt caaaaattta ctcgaattgt
     1861 gcgttctggc aagcggactg acgcgaaagg agagtataac ggagcccccc ggccagatga
     1921 acacggagtg gagcctcccg cgacaccggg catccactgt gtgccgcgac cgggcgaaac
     1981 cgagacagcc cgaggccggt ttgccgtcgc ctccggcctg ggttaggcta tcgggctgaa
     2041 ttgcgtctgc ctggcgctga ccggcggctc accggagcgg gtgaggagca ccggagcgga
     2101 tagaatgcgc cggcaaccgg ccaatcgatc ggatctcaag gaggataacc atatgagcga
     2161 tctcgtgcag cgcctcggaa cggagcagga cgtcgaggtc agcatgaggc ccgagacgac
     2221 ggtggagtac ttcaagcccg ccgtcgaccg cggctacgtc cacatcaagt tcatccatac
     2281 ccgcggcggc accgagctcg gcatccgcct cgaccgggac gccagcgact tctcccaggc
     2341 cgactgggag aacaagaccg gcaaggtgaa gatcgtcggc aacctgatcc tcgactacgt
     2401 caaggtgcgc ttccacggca ccatcgacct gtcgaccctc aagggcaccg gctatctgga
     2461 gaacctgggc gagtacaagc cctgggaaga gaaggcggcg gctgaagcca cctagcgtcc
     2521 ggagcggggc cgggaacctg ccagctcggg gatcccggcc tcggccagcg cctccgagag
     2581 cctctccccc cctttgacgg cgaagtccag cgtgcgagtg gggaacggga tggtgatccc
     2641 ctcggcgtcg aaggcgcgct tgagcctctc gatcgcctcg ctgcgcgccg ccatgtagtc
     2701 gcgctgctgg ctgaagggga tccagaaccg caccacgaag ttgatggagc tctcgccgag
     2761 ctcctcgtag aacagctcca ccggccggtc cggggtccgg ttggagacct cctccagcgc
     2821 ccggatggcc acctcccgca cccgcgccag atcgtcgccg taggagaccc ccaccgacag
     2881 gtccacccgc cgccggccgg tcgtggtgta gttcatgagg gggttttcga acacctgctt
     2941 gttggggatc agcacgagct gcccctgctg ggtacgcatc tcggtccagc gcaggtggat
     3001 gcgctccacg gtgccgaagt agtcctgggt cttgatgaga tggccgtggc ggaaagggcg
     3061 cttgaccgag agatagatcc ccgccatcag gttggcggcg atgtcctgga aggcgaagcc
     3121 gagcgcgagg ccgacgatgc ccgcgccggc gagcagcgac gcaaccgtct tttgcaggcc
     3181 caggatgccg agcgccgcga acaggccggc cgccacgatg ccgatgtaga cggcttggct
     3241 gaccagccgg ttgacctggt cgctgtgaga gacccggtgc aggaaccgat ggacgaggct
     3301 gcgcaccgcc cgggccacca gccaagcgcc gaccaggacc agaacggcga cgacgaggtt
     3361 cggcagatgg aggagcagag cctcgatcca gctccgcacc ttcccggtca gccgggccca
     3421 gacgcgctcg aagttgacca tccgtccccc ctcggagaac gggcagcttg cgaatttctt
     3481 gcctaccgcg cgagacgcgc ttcgaggccg gcccggacct ccggccactc gccgtcggtg
     3541 atgctgtaca tcaccgtgtc gcggaaggag ccgttggcgc gcatctgata gcggcgcagg
     3601 accccctcgc gccgggcgcc gagcttctcg atggcgcgct ggctgtggag gttgttgaag
     3661 tcggtcttga actggacccg cacggcgccg agggcctcga aggcgtggcg gagcaggacg
     3721 tacttgctct ccgggttgac ccgcgtcccc tgccagcgcc gggcgatcca cgtccgcccg
     3781 atctcgagcc cgcggtgggc cggccggatc tccaggtagg ccgagcaccc caccggctcc
     3841 ccggtctccc gcaggcgcag gacgaagtcg aggctcgc
//
LOCUS       DASPJI010000580         6003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_670307, whole genome shotgun sequence.
ACCESSION   DASPJI010000580 DASPJI010000000
VERSION     DASPJI010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670307"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1050..1391
                     /locus_tag="VEL74_16200"
     CDS             1050..1391
                     /locus_tag="VEL74_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64118.1"
                     /translation="MNTRKDGTPTPPSDSVDLSQVTEDTRRKLYDAAVATHFTPTPEE
                     KAQADPENHWQPPAYTPDQARLTVHELYGRWFVTWRRLEEDGDVAPYRQWAVLRVVQD
                     PERGTLEYYEV"
     gene            complement(1814..2821)
                     /locus_tag="VEL74_16205"
     CDS             complement(1814..2821)
                     /locus_tag="VEL74_16205"
                     /inference="COORDINATES: protein motif:HMM:NF016629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HYG64119.1"
                     /translation="MGPTDGRFKLFFSYRRMMKDLVLGFLREGWLHGLDLATLEMVPS
                     TFITRRFRQRHGDCLWRARFDGPDGTSTDFYLLVEFQSKPDRYMPLRLLGYEALLLER
                     LVRRQELAAGGDLPPVVALVVPSGKRHWNVPRELRTQFAPVPEEAARLLPSLSYLLID
                     RTLLETGALDPQDNLAAVFFRIEEARSPEDLLSLARPLHRMLPAKSEPELRRAFTDLL
                     VDTFQTAFPGVTIPPIEDLEDMSMLEENMIRWRNKVIREARKEGLKEGRQEGIKEGRQ
                     EGKVDGMRSFVLGLMEQRFGPLSPTVRRKVDKISSAEQLQDLGNRLLTAGSLSDLGLG
                     G"
     gene            complement(2998..3264)
                     /locus_tag="VEL74_16210"
     CDS             complement(2998..3264)
                     /locus_tag="VEL74_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64120.1"
                     /translation="MTRNRHLTRMLIKLAVPVAVLVAGVTTGAADRPQNGCTYGRYYI
                     YYDAQGSICAEQETCTNTGWGDCDDNETYVSSEDFPHVCYCDPQ"
     gene            complement(3321..>6003)
                     /locus_tag="VEL74_16215"
     CDS             complement(3321..>6003)
                     /locus_tag="VEL74_16215"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HYG64121.1"
                     /translation="RQGTANVAGTAEDYRAEVRAHLAPSPQVAGGKIAPGNWSITGEG
                     DPNSFRFSSLQGDLFSGRVTGQGEVAWKPAVRWKAALRGARINPQVLSASFPGNLAFS
                     ATSQGRLTDAGPVGTVQVPSLAGTLRGEPIRAVAGVELAGDRYRISKLDATWSTAHLT
                     ASGWLGDAYDLAFNLDAPNLAVAVPQGAGAVTAQGRITGPAETPRIQATVQGEDIRFG
                     TQTVAAVEAVADVDLAPTGPFTLDATANGFQSGERRVEDLVLRGRGTRSNHTITATVN
                     STDGNVDLALAGGLQGTTAWQGTIQRLGLQSEMVGNWTLGRPAPLFASAEEVRLRDFC
                     WQSQGGQVCATGGWAKAGPWNVDSTLSDLPLRMLKPMLPPDLEITGALNGSVQARGAG
                     SAVASAVVDIRPGPGEFRFPGNEGRTVAFRYEQGSIQAQVGAGGEGTASARLVLVDAG
                     TLSAQARLPRFVRGASLQAQPLAGRVDVNLTNLAFIQGFVPDVDNPGGTLTAGYALSG
                     TVGAPRLQGEARLANAQADIPRLGIELRDLRLAAIGDGSGSLTLDGSARSGPGTVSIT
                     GRAGLVPSAETPIKLAIKGQRFQAMDTEEIDVLVSPDLSVDYTGKLVQLTGDVVVPQA
                     DIVIEDRREKGPVTVSEDVVFVNATEEPDARQDLAVVTRVRLVLGRDIDINVYGLRGK
                     PTGSLLVRDEPGRVTRGTGELEIKEGIFKAYGQDLTIERGRIVFAGPIDNPGIDVRAF
                     RKAEDGTIAGINAKGTVQKPEVTLWSQPTLPESEALAYLLLGRPLNQTQPQEGDRLAN
                     AATSLGLKGGNMLAKKLAARWGLEEARIEAKDNDLDQASLVVGKYLSPRLYVAYGIGL
                     FEAVNTFRIRYLMNEKWTLQAESGEGTSADVLYTIER"
BASE COUNT          926 a         2016 c         2095 g          966 t
ORIGIN      
        1 ctggtgatct gtccggtgac ctatacggag ctggccccct ctttagaggg tgacaccgcg
       61 cgccagggcg agttcctggg ccggagtcgg cgccgatttc cggcaggact ggctctggga
      121 tgacaccttg cgggcgcaca aggcctggca cgacttcatc cggcggagga agaggtcccg
      181 ggatctcccg aagagacctc tggcggacat cctgatcggg gccttcgcca tgcgccacca
      241 ggggctgatc acccgcaacg agacggattt cggatcggtg ttcccgacct cacgctccgt
      301 ggcgtcctgc cggagtaggc gagcgacccg cccgtgtcgc cgcacaccct ggtgctggcg
      361 atgacaccgc gctgaacttc gaaaaggtca ggctctacgc ctccgacagg cagcgccgca
      421 ccggaggatc gagtaaagca cggctgggcg ccgcccccgc ggcgcccgaa ccgttccatc
      481 gattgtcaaa gaaccgtctg acccgcgccg gatcggtctc gacccggcgc ggacctctct
      541 cgaccctccc caaaccaccc gcgaccccgg ggtcgagagg ctcgaccctc tccaaacctc
      601 tctcgacccg gctcggacct gacgcgaccc tagggttgag agacttgacc ctcgggtcac
      661 atcagctctg tgagctcaga agccacgatt gagtcaccgc tgctcagtga tctgacagct
      721 ctatgagatc gaagtctgcc gggaaagtca attatggtct tgcggtcggc tcagggctcg
      781 tgtagagtag gtcaccgcta agctctgccg aagtttacct cgagagaggt cgcagggagg
      841 gtctggggag agattagaac cgggtggata cgttcgacct cttgcatcca cccggaacgt
      901 tgcgctatac tgctgctcgt cgtcgttgta agcagtgtgt cgctggaccc gtggggattt
      961 cccccgtggg aatcagcacc agcggctccg gatgaaggct cgcatcctta cacccggagc
     1021 ctaggtgccc gaaaggagta gtgagctcca tgaacacccg taaggatggt acacccaccc
     1081 ctccgtccga ctccgtggat ctctcccagg tgacggagga cacccggcgc aagctgtacg
     1141 acgccgcggt ggccacccac ttcactccga cgccggagga gaaggcgcag gcggaccctg
     1201 agaatcactg gcagccgccc gcctacacgc cggaccaggc gcgcctgacc gtccatgagc
     1261 tctatggacg ctggttcgtg acctggcgcc ggctggagga ggacggagac gtcgcgccgt
     1321 accgtcaatg ggccgtcctc cgtgtcgtcc aggaccccga gaggggcacc ctggagtatt
     1381 acgaagtctg agttgtacag ccgccgggga gccgaggttc cccggcggcg cggcctcccg
     1441 ccgacccctg gggctgacgg ccaggctgag aaaagggccg cctcctgcgg aggcttgtga
     1501 atgagtcttg agcccccgca ggggccgacc tttttttcag accgggccgt tagccccggg
     1561 gagtgcgcgc cactttggtg cacggtttta gacgcccgtc tccggagacc ggagccggga
     1621 gggcttacag ggttcattga caatcgtcgt acgcaaaagc gagagagggg cttcgccgct
     1681 tagtggccag gggcccctcc cgcggcagcg tcactgggga tcgaattagc agacgtgcgg
     1741 gggggagtcg acacccggag taggtgaacg acccactcgt gtcgccgccg cacggtgacc
     1801 gcggcgcacg gggctagcct ccgagcccca ggtccgacag gctgccggcg gtgaggagtc
     1861 ggttgcccag atcctggagc tgttccgcgg acgagatctt gtcgaccttg cggcggaccg
     1921 tcggagacaa gggcccgaag cgctgctcca tcagacccaa cacgaagctt cgcatgccat
     1981 cgaccttgcc ttcctgacgg ccctccttga ttccctcctg gcggccctct ttgaggcctt
     2041 ccttgcgggc ctcccggatg accttgttgc gccagcggat catgttctct tccagcatgc
     2101 tcatgtcctc cagatcctcg atagggggga ttgtaactcc tgggaatgcc gtctgaaagg
     2161 tatcgaccag tagatcggta aaggcccggc gcaactctgg ctcgctcttc gcgggcagca
     2221 tccggtggag tggccgtgcc aggctcagca ggtcctcggg cgagcgggcc tcctcgatgc
     2281 ggaagaacac cgccgcgagg ttgtcctgcg gatcgagggc tccggtctcc aggagggtcc
     2341 ggtcgatgag caggtaggac aagctgggca gcaggcgcgc ggcctcctcc ggtacgggag
     2401 cgaattgggt ccgcaactcc cgggggacgt tccagtgccg tttgccgctc gggacgacga
     2461 gggcgacgac cggcggcagg tcgccgccag ccgcaagctc ctgccgccgc accagccgct
     2521 cgagcagcag ggcctcgtag ccgagcaggc gcagaggcat gtagcggtcc ggcttggact
     2581 ggaactcgac gagcaggtag aagtccgtcg aggtcccgtc cggtccgtcg aagcgggccc
     2641 gccagaggca gtcgccatgc cgctgccgaa acctgcgggt gatgaaggtg gagggcacca
     2701 tctccagggt cgccaggtcc agcccgtgga gccacccttc ccggagaaat ccgaggacga
     2761 ggtccttcat catgcggcgg tacgaaaaga agagcttgaa acggccatcg gtaggcccca
     2821 tgcgtggtcc gtctcctttc agggccaccc ctcccgagag aagagggtgg cggtccgaaa
     2881 ggggaaagac ggaaaacgcc ggaaaaatcg ctcacttttc ttcagggagg tgggagatac
     2941 ccccagcgtc ccggctccgg gagaacggag ccgggacgga ttcactgacc gtcgttgtta
     3001 ctgggggtcg cagtagcaga cgtgcgggaa gtcctcggag gacacgtacg tctcgttgtc
     3061 gtcgcaatcc ccccagccgg tgttggtgca ggtctcctgc tcggcgcaga tgcttccctg
     3121 tgcgtcgtag tagatgtagt agcggccgta ggtgcaaccg ttctggggcc tgtctgcggc
     3181 gccggtggtc acgcccgcga cgaggacggc gacgggcacg gccagcttga tgagcatccg
     3241 agtaaggtgg cggttcctgg tcatggtctc tccttctgtt caaggcgagc ttaccaggcg
     3301 atcggctgga gatctaagcc tcaccgctcg atcgtataca gcacgtccgc gctggtgccc
     3361 tcgccgctct ccgcctggag ggtccacttc tcgttcatga gatagcggat cctgaaggtg
     3421 ttgaccgctt cgaagaggcc gatgccatag gcgacgtaga ggcgcggcga gaggtacttg
     3481 ccgaccacca gcgacgcctg gtcgagatcg ttgtccttgg cctcgatgcg cgcctcctcg
     3541 aggccccagc gggcggcgag cttcttcgcc agcatgttgc cgcccttcaa acccagcgag
     3601 gtggcggcgt tggccaagcg gtccccctcc tgcggctgcg tctggttgag cggccggccg
     3661 aggagcaggt aggccagggc ctcgctctcc ggcagggtgg gctgcgacca gagggtgacc
     3721 tcgggcttct gcaccgtccc cttggcgttg atccccgcga tcgtgccgtc ctcggccttg
     3781 cggaaggccc gcacgtcgat gccggggttg tcgatcgggc cggcgaagac gatgcggccg
     3841 cgctcgatgg tgaggtcctg gccgtaggcc ttgaagatcc cctccttgat ctcgagctcg
     3901 ccggtgccgc gggtcacccg cccgggctcg tcgcggacca gcagcgagcc ggtgggcttg
     3961 cccctcaggc cgtagacgtt gatgtcgatg tcgcggccga gcaccagccg cacccgcgtc
     4021 accaccgcga ggtcctggcg ggcgtccggc tcctcggtgg cgttgacgaa gaccacgtcc
     4081 tccgagaccg tgaccggtcc cttctcgcgc cggtcctcga tgacgatgtc ggcctggggg
     4141 accaccacgt cgccggtgag ctgcaccagc ttgccggtgt agtcgacgga caggtccggc
     4201 gagaccagga catcgatctc ctcggtgtcc atggcctgga agcgctggcc cttgatcgcc
     4261 agcttgatcg gcgtctcggc gctcggcacc aggccggcgc ggccggtgat cgacaccgtg
     4321 ccggggccgg agcgggcgct gccgtccagg gtcaggctgc cgctgccgtc gccgatggcg
     4381 gcgagacgga ggtcgcgcag ctcgatgccg aggcgcggga tgtccgcctg ggcgttggcc
     4441 agccgcgcct cgccctggag acggggcgcg cccacggtgc cggagagggc atagcccgcg
     4501 gtcagcgtgc cgccggggtt gtcgacgtcg ggcacgaagc cctggatgaa ggcgaggttg
     4561 gtcaggttga cgtccacccg gccggcgagc ggctgggcct ggagcgaggc gccgcgcacg
     4621 aagcggggga gccgcgcctg ggcgctcagg gtgccggcgt ccaccagcac cagccgcgcc
     4681 gaggccgtcc cctcgccgcc cgcacccacc tgagcctgga tcgagccctg ctcataacgg
     4741 aacgccacgg tccgcccctc gttgccgggg aaccggaact cacccgggcc gggacggatg
     4801 tccaccaccg cgctggcgac cgccgagccc gcgccacgcg cctgcaccga gccgttcaac
     4861 gcgccggtga tctcgaggtc ggggggcagc atcggcttga gcatgcgcag ggggaggtcg
     4921 gagagcgtcg agtcgacgtt ccacgggccc gccttggccc agccgccggt ggcgcagacc
     4981 tgccccccct gggattgcca gcagaaatcc cggaggcgga cctcctccgc cgaggcgaag
     5041 agcggcgccg gccgtcccag cgtccaattg cccaccatct cggattgcag gccgagccgc
     5101 tggatggtgc cttgccaggc ggtcgtcccc tggagcccgc cggccagggc caggtccacg
     5161 ttgccgtcgg tgctgttcac cgtggcggtg atggtgtggt tgctccgcgt gccccggccg
     5221 cgcagcacca ggtcctccac ccggcgctcg ccggactgga acccattcgc cgtggcgtcg
     5281 agggtaaacg gaccggtggg cgcgaggtcg acgtcggcca ccgcctcgac ggccgcgacc
     5341 gtctgggtgc cgaaacggat gtcctccccc tggacggtcg cctggatgcg cggcgtctcc
     5401 gccggcccgg tgatccggcc ctgcgccgtg accgcgccgg ccccctgcgg gaccgccacc
     5461 gccaggttgg gcgcatcgag gttgaaggcg aggtcgtagg cgtcccccag ccagccggag
     5521 gccgtcaggt gcgccgtgct ccaggtggcg tcgagcttgg agatacggta gcggtcgccc
     5581 gccagctcca cgccggccac ggcccggatc ggctcgccgc gcagcgtccc cgcgaggctc
     5641 ggcacctgga cggtgcccac cgggccggcg tccgtgagcc gcccctggct ggtggcggag
     5701 aaggcgaggt tgccggggaa cgacgcggac agcacctgcg ggttgatgcg ggcgccgcgc
     5761 agcgcggcct tccagcgcac cgcgggcttc caggcgacct ccccctggcc ggtgacgcgg
     5821 ccggagaaga ggtccccctg gaggctggag aagcggaagc tgttcggatc cccttcgccg
     5881 gtgatgctcc agttgccggg cgcgatcttg ccgccggcga cctgggggga gggggccaga
     5941 tgggcgcgga cctcggcgcg atagtcctcg gcggtgcccg cgacgttggc ggtcccctgg
     6001 cgg
//
LOCUS       DASPJI010000581        17100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_264098, whole genome shotgun sequence.
ACCESSION   DASPJI010000581 DASPJI010000000
VERSION     DASPJI010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17100)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17100
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264098"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VEL74_16220"
     CDS             <1..234
                     /locus_tag="VEL74_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012409959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64122.1"
                     /translation="DQDGYAFIGKVRAAGKRLPAVALTAYARVEDRLKALVAGYNMHV
                     PKPVEPAELAVVIASLVGKDQTQRDPGKQKQAR"
     gene            complement(267..3428)
                     /locus_tag="VEL74_16225"
     CDS             complement(267..3428)
                     /locus_tag="VEL74_16225"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HYG64123.1"
                     /translation="MRLLRFPAIHGDTVVFTYSSDLWVADRRGGHARRLTTHRGEERR
                     SRISPDGKLVAFSASYDGNADIYVMPLEGGAPRRITFDPFMESLVGWTPDGKVAYGSP
                     AGSFTPSQPRLWVAEPAGGLPRALPVREITNGSFFPDGRRIAYNRTDAYLRWRRYRGG
                     LVGKVSIYDLASNTYSELPGGNENSWFPMVAGNGNSIYYVSDRTGGTVNLYRHDLATK
                     QDTQLTSYTDADIKWPSSDGKTIVYEHDGYLHAFDTATGKDERLTFQVRSDELAARPY
                     MLKAGKTISDISPSPSGKRIAVEARGEIFNVAAKGVDARNLTNTPGVRERWPRWSPDG
                     KTIAYLSDATGEYQIYGQPATGSGAGTAKQLSRHTGAGIYGFFWSPDSKWIVYVTQDY
                     RFLLLDAETGKTTQIFRAEYQEGADFDWSPDSRWIAFSNTVANGFGAIHLYEVETGKT
                     TRVTDGFYDDRNVNFDLSGKYLYFTSARTFTPSFGRFEASLKVDDADRIYVMPLARDE
                     RNPLFPKAEEESVPKDETAEEKGEAKKEEEKSDTVRVRVDLEGLAGRAVVLPIPAGDP
                     GALFGGKEAVFHYAEGKLRKFDLASRETATIYDGPEIDLEFTPDRSKLAYFGKEVLGV
                     ADVKPDTKHGAGKVDTAAVEVWVDPAAEWRQMYWEAWRFERDNFFREDMWGLDWPAVG
                     KHYEQFLPYVTHWSDLKYVLSLLLAELGTSHAYIYDPPAEPGAADRAASTAMLGADYE
                     LDGRWVRFQRIYPGTLTQDAWRGPLGEPGVNIKTGDYLLAIDGRPVDAGTHPNALLIN
                     KADRVVTLTVNEKPSFEGARKVEVRPIAQELDLRYYEWVEENRRKVHEMSGGRIGYIH
                     YPNTGTAGQTEFIRGYWAQTDKEAIILDERFNGGGFPQPMVLPTLGRRAQTIVISRKW
                     RHGSEVRAINGPKAMLINGYAGSGGDLTPWMFRDAGMGTLIGTRTFGGLVGIADAVDL
                     VDGTRVTAPGYTRFDPETGEWIAENKGIAPDIEVDNRPDLIAQGRDPQLETAVQRLLK
                     ELEERPLKVVEPRLPPPPAKRGQ"
     gene            3724..5055
                     /locus_tag="VEL74_16230"
     CDS             3724..5055
                     /locus_tag="VEL74_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HYG64124.1"
                     /translation="MLARPQARAAVLSVLAALAIPGTVLLGAPPPASAQTDLQREVDR
                     RATEVLPKVIAWRRDIHQNPELGNRETRTSALVADHLRKLGYEVRTNVAHTGVVGILR
                     GGKPGPVVALRADMDALPVTEQADVPFASKVRTTYNGQEVGVMHACGHDVHTAVLMGA
                     AEALAGVRQELPGTVKLIFQPAEEGVPPGEEGGAKMMMAQGVLADPAPQAIFALHTTT
                     LPGGTLAYKAGSIYASADVLKIVVRGRQTHAAMPWQGIDPVILSAQIILALQTIPSRQ
                     LDPFLPNVVTISTVHGGVRNNILPDEVVMEGTIRSLNPALHEDFLARIRRTAESIATN
                     AGATAEVTITPYAPVTVNDAALTRRMEPTLQRIAGTSLSGTPGSMAAEDFSFFAQEVP
                     GLYVFLGINPPGVEPGEAAPNHSPRFFVHEDSMVVGIRTMAGLAVDYLMGE"
     gene            complement(5108..5467)
                     /locus_tag="VEL74_16235"
     CDS             complement(5108..5467)
                     /locus_tag="VEL74_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64125.1"
                     /translation="MNTRDNRTPTPPSEPSKSAVDLTQETEDTRRALYDAAVATHFTP
                     MPEEKDKAVADPESWQPPAYTPEQARLTVHQLYGRWFITWRLLEEKEGEVPPSRLWTV
                     LRVLQDPDRGILEYHEV"
     gene            5805..6170
                     /locus_tag="VEL74_16240"
     CDS             5805..6170
                     /locus_tag="VEL74_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64126.1"
                     /translation="MAEAGGATSLWTLEADGTFFATSSLQALLKFSDQQGSWEKVGND
                     GIKGVVLALVFDENDALLNVSRVDISLHTVGRGCDNIAGSLEVREFTGAEDPLDPATD
                     TGEPIATDTVTGRRVKANR"
     gene            7024..8394
                     /locus_tag="VEL74_16245"
     CDS             7024..8394
                     /locus_tag="VEL74_16245"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015754569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HYG64127.1"
                     /translation="MIDQAAETILETGRSGMPPRPAFDVRRLGPARLPSPLSGRRERF
                     IGDGERVLATTDAVRIADYLEGEGALPCFERAGPRRELFFDPRTLGAGIVTCGGLCPG
                     LNNVIRSIVLNLYYAYGVRRILGYRRGYLGLSRRRPEEPLELGPAQVESIHERGGTIL
                     GTSRGPQDVGEMIDTLQRDGVQILFTLGGDGTLRGAAALAREIERRGLAISVIGVPKT
                     IDNDIAWVDRSFGFTTAIEEGNRAIHAAHAEAQAAWNGIGMVKLMGRHSGFIAAHATL
                     SNNDVNFCLVPEVPFSLHGHGGFLEALEQRLQERRHAVIVVAEGAGQELLRREGQVER
                     DASGNVRLLDVGVYLRDRITEHFSSQGMDVTIKYIDPSYIIRSQPANSSDAELCLFMG
                     EHAVHAGLAGRTEMVVGYRNQQFIHLPIPLAVARRKSLDRRGKIWEGVLGTTGQPVSM
                     LGDSAI"
     gene            complement(8678..9601)
                     /locus_tag="VEL74_16250"
     CDS             complement(8678..9601)
                     /locus_tag="VEL74_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64128.1"
                     /translation="MRGDRIVGAVLLVLAGVPGAALAGGAWVPEPGQGDVQLGYSQKK
                     ASTSWNAFGDSFTNTTVVDGEVKRHYHDFRYGYLSGEVGLLPRLSTTFLLTYLHGLEG
                     PGDHLEKNTGPSDAWFGLKYALVQGDLPMALALTYRTPYFYDLEGPYSRYLYDSNGNI
                     RDVSPEWRGVLKHDLTLSYLVSRPFREGHGWFNLQAGYTWREGAPADEVPVWGELGWP
                     LPLWKLQGKVAAVYIGSLGNDSPRQPDDRFGSRPDFNFNDASMGRIGVGIIAPLDSAG
                     RWSVEAGYNQWVWGESARRYDEPYLSLGRRF"
     gene            9708..10571
                     /locus_tag="VEL74_16255"
     CDS             9708..10571
                     /locus_tag="VEL74_16255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYG64129.1"
                     /translation="MPRHPLLLMVLSSACFAFMALAAKLAAGGLSGGQVAFGRFAFML
                     LPVVLVPPLARKAVTFQRLDLLFYRGLFGGSAVLLYFFALEHMPVGIATLLNYSSPIF
                     SVAFAALFLGERVDGRLLLPLIAALSGMVLAAGGGGGLGGIRFGLWEALGMASAVLSG
                     AAVTAIRAARRTEGSWAIYGSFTIFGLLATAPFGARGLGAATSREWLLLAAVGAFSIV
                     AQLLMTYAYRWVTNLQAGVVNQLTVVLSLLLGVVFLGDRLAPVQIVGSLLTLAGVVGV
                     VAVQSTPRAVE"
     gene            10667..11452
                     /locus_tag="VEL74_16260"
     CDS             10667..11452
                     /locus_tag="VEL74_16260"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HYG64130.1"
                     /translation="MRLALVNVKGGVGKTTTAVNLAAAFARGGLRVLLVDLDPQGSAS
                     LSLGVGRAETQDQPTVAELLTGEAEAAAAVRQTGAAGIDLIPSALRLAAMDLALAREA
                     EPGKLLKKALAPLRRRYDVTVMDCPPGLSILSVNGLTAAQAFIVPVAPHHLDMDALAG
                     FFELLEMERGRFGRKTELLGIVLTMVDHRTRVTDEVVAAIRKSYGKQVFKSEIPINVR
                     LAEAPGHGRTIFDFESWSTGALAYSHLGGEVLRRARKAGLLES"
     gene            complement(11489..12040)
                     /locus_tag="VEL74_16265"
     CDS             complement(11489..12040)
                     /locus_tag="VEL74_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HYG64131.1"
                     /translation="MKRIVLRFGLASGVILVALSSVLWAVTMNEAIDLEGSEVIGYSA
                     MVLSFLLVFFGVRSYRDTVAGGAIGFGKAFQVGLLITLITCAMYVITWEIAYFNFFPD
                     FLDEYTTRALAKMRAEGESAAAIQKMEGQMAFMAKYYDNIFFNSAITLLEVFPVGLIV
                     SLISAAILRRKPAEAAPAATAIA"
     gene            complement(12179..12664)
                     /locus_tag="VEL74_16270"
     CDS             complement(12179..12664)
                     /locus_tag="VEL74_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HYG64132.1"
                     /translation="MRRVILYGLVGGVLITLLKVLEYKHFVRAYPTEIYGGLVAVIFT
                     AVGIYLGLRWSRPRQPERTAEPAEPREVVVREVVEVRVREERPFVLDPGKLAELGITP
                     REHEILGLIAEGLSNREIGERLFVSENTVKTHSSRLFGKLGVNRRVQAVQRGKELGLI
                     P"
     gene            complement(12746..12991)
                     /locus_tag="VEL74_16275"
     CDS             complement(12746..12991)
                     /locus_tag="VEL74_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64133.1"
                     /translation="MKRFVLSSAAFALVAATQVGTASSNHHCPPPQQYSGSCSQVIVW
                     AQSPDKERCCQYSTPCAAPSGWTIFYGPGCTTTTVEL"
     gene            complement(13089..13955)
                     /locus_tag="VEL74_16280"
     CDS             complement(13089..13955)
                     /locus_tag="VEL74_16280"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HYG64134.1"
                     /translation="MGCGRTWTAAALLCLAGIAPSKTAGQTTQTPPEVPTPPIPPVIV
                     EVPGEVPAAVAPAGTPTRERTYRSMPLTGGEKELRALFNPEQLALLEKLNRRDVPHLA
                     RAKTLVVPEEWLPDELLYSPLPLSWDWAAQHPKALVVHQPSQVFGAYEAGLLVRWGPV
                     SSGREKNPTPAGLFHLNWRSKGRRSTDNPDWYMPWYYNFNTRRGLAFHQLELPGRPAS
                     HACIRLLERDAVWLYSWGQGWTLDESRRHVLTPGTPVLILETYDFAASPPWLSLDWLA
                     AGIELPVEPPKS"
     gene            complement(14082..14441)
                     /locus_tag="VEL74_16285"
     CDS             complement(14082..14441)
                     /locus_tag="VEL74_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64135.1"
                     /translation="MSRISRWSLLGLCLVLALGMALPSQAAPASTSNALFLAELSEQA
                     PCQLEPSSLVAKPDENKPVFKVIYPDCGDLCSDTRCAGKPTLSQCTTASGQPGFCHAV
                     NQVCPNEPRRSPCTCRT"
     gene            14624..15214
                     /locus_tag="VEL74_16290"
     CDS             14624..15214
                     /locus_tag="VEL74_16290"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYG64136.1"
                     /translation="MATVTGPSAQTLPDDLAALRRQVEDNRRTAEDLVRGLSEAQLQW
                     RPDERSWSIAQCLDHLAIGDRAYLEAMSPAVAEARRRNSRRRGPIQPGWFARWFIQSM
                     EPPPRRKLPAPRKIVPSLGKSGDEVKRDYFAAQAGVLALLDQAAAVDLNRTRFVNPFL
                     PLLRFTVGTGFLVSVAHGRRHLWQAERIRQRPGFPP"
     gene            15235..15639
                     /locus_tag="VEL74_16295"
     CDS             15235..15639
                     /locus_tag="VEL74_16295"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HYG64137.1"
                     /translation="MTDEPQRRRWARVVLRVAVALLLIIHGVARASLGIVDDFGGFLA
                     SLGLPFGTAVAWLLTVVEIAGGLALAAGFLVLPLCVWFAVELATGIALVHAPNGWFVV
                     GAGRNGVEFSVLLIVCLVVITLEHPGRRRTSP"
     gene            complement(15649..>17100)
                     /gene="polA"
                     /locus_tag="VEL74_16300"
     CDS             complement(15649..>17100)
                     /gene="polA"
                     /locus_tag="VEL74_16300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HYG64138.1"
                     /translation="LASYLIKPSLHGHSLDEMALERLSRKAISAKESGWDKGQEPPQG
                     DSRLAEYAAERVAFARRMAGTVRRDLEDAGLDTVYREIEAPLVPVLLGLEEAGILLDT
                     GFLRDMSAELGQELETLEEEICQQAGERFNLNSPAQLGTILFEKMKLPVLKRTQKTKS
                     YSTGAETLEELAGRGYPIAQYLLRYRELIKLKSTYIDALPTLVGPDGRLHTRFNQAVA
                     ATGRLSSTNPNLQNIPIRTELGQRIRKAFIASPGNVLLVADYSQIELRILAHIAEEKE
                     LIRAFLAGEDIHRSTAATVFGIAPELVTGDQRRAAKTINFGILYGMSPFGLSQALGIS
                     TKEADHFIRAYLDRFAGVRTYMEETQASAEKEGKVTTLYGRVRWLPEIQSKNRSLREN
                     ARRMAINARIQGTAADLLKKAMIAIDCRLRQEHPEARLLLTVHDELVFEVPESQAEAI
                     AGPVRQEMEGVAHLKVPLVVDVGWGRSWYEAKA"
BASE COUNT         2531 a         5937 c         5895 g         2737 t
ORIGIN      
        1 gaccaggacg gctacgcatt catcggcaag gtgagagccg ccgggaagcg gctgccggcg
       61 gtcgctctga ccgcctatgc ccgggtcgag gaccgcctga aggcgctcgt cgcgggctac
      121 aacatgcacg tccccaagcc ggtcgagccc gcggagctgg ccgtggtcat cgccagcctg
      181 gtcggcaagg atcagacgca gcgcgatccc gggaaacaga agcaggcgcg gtaggccggt
      241 gcccccgcgc ccgccggtcc cggcgtctat tgccccctct tggcaggcgg cggcggaagc
      301 ctgggctcca ccaccttgag cggcctctcc tccagctcct tgaggaggcg ctgcacggcc
      361 gtctcgagct gcgggtcgcg gccctgggcg atcagatccg gccggttgtc cacctcgatg
      421 tccggcgcga tccccttgtt ctccgcgatc cactcgccgg tctccggatc gaaccgcgtg
      481 tagccgggcg cggtgacccg cgtgccgtcc accaagtcga ccgcgtcggc gatgcccacc
      541 aggccgccga aggtacgggt gccgatgagc gtccccatgc cggcgtcgcg gaacatccac
      601 ggcgtcaggt cgccgcccga gccggcgtag ccgttgatca gcatcgcctt cggcccgttg
      661 atcgctcgca cctccgagcc gtggcgccac ttgcgggaga tcacgatcgt ctgcgcgcgg
      721 cggccgagcg tcggcaggac catcggctgc gggaagccgc cgccgttgaa gcgctcgtcc
      781 aggatgatcg cctccttgtc cgtctgcgcc cagtagccgc ggatgaattc ggtctgcccc
      841 gccgtgccgg tgttcggata gtgaatgtag ccgatgcggc cgccagacat ctcgtggacc
      901 ttgcgccggt tctcctcgac ccactcgtag taacggaggt cgagctcctg ggcgatcggc
      961 cgcacctcaa ccttgcgggc tccttcgaag gacggcttct cgttgaccgt gagcgtcacc
     1021 acccggtcgg ctttattgat caatagcgcg ttggggtggg tgccggcgtc gaccggccgg
     1081 ccgtcgatcg ccagcaggta gtcgcccgtt tttatattga cgccgggctc gccgagcggg
     1141 ccgcgccacg cgtcctgcgt cagcgtgccg ggatagatcc gctggaagcg cacccaccgg
     1201 ccgtcgagct catagtcggc gcccagcatg gcggtgctgg ccgcacggtc ggcggctccc
     1261 ggctcggccg gcggatcata gatataggcg tgggaggtgc cgagctccgc gagcagcagg
     1321 ctcagcacgt atttgagatc gctccagtgg gtgacatagg gcaggaactg ctcatagtgc
     1381 ttgcccaccg cgggccagtc gagaccccac atgtcctcgc ggaagaagtt gtcgcgctcg
     1441 aagcgccagg cctcccaata catctgccgc cactccgcgg ccgggtccac ccacacctcg
     1501 acggcggccg tgtcgacctt gcccgcgcca tgcttggtgt cgggcttgac gtcggccacc
     1561 cccagcacct ccttgccgaa ataggcgagc ttcgagcggt cgggggtgaa ttcgagatcg
     1621 atctccgggc cgtcgtagat cgtcgccgtc tccctggagg cgaggtcgaa cttgcggagc
     1681 ttgccttcgg cgtagtggaa gacggcctcc ttgccaccga acagcgcccc ggggtccccc
     1741 gccgggatcg gcagcaccac ggcgcggccg gccaggccct ccagatcgac gcggacgcgc
     1801 accgtatcgc ttttctcctc ctccttcttc gcctcgccct tttcttcggc cgtctcatcc
     1861 ttcggaacgc tctcctcctc cgctttggga aagagagggt tgcgctcgtc gcgggccagc
     1921 ggcatcacgt agatgcggtc ggcgtcgtcg accttgaggc tcgcctcgaa gcggccgaac
     1981 gacggggtga acgtccgcgc cgaggtgaag taaaggtatt ttcccgacag gtcgaaattg
     2041 acgttgcggt cgtcgtagaa gccgtcggtt acgcgcgtcg tcttgccggt ctccacctca
     2101 tacaggtgga tggcgccgaa gccgttcgcg acggtgttgg agaaggcgat ccagcggctg
     2161 tcgggggacc agtcgaagtc ggcgccttcc tgatattcgg ccctgaagat ctgagtggtc
     2221 ttccccgtct cggcatcgag cagcaggaac ctgtagtcct gggtgacgta gacgatccat
     2281 ttggaatccg gcgaccagaa gaagccgtag atgccggcgc cggtgtggcg gctgagctgc
     2341 ttggcagtac ccgcgccgga gccggtggcg ggctggccat agatctgata ttcgccggtg
     2401 gcatcgctca gataggcgat ggtcttgccg tccggcgacc agcggggcca gcgctcccgc
     2461 acccccggcg tgttggtcag gttgcgcgcg tccaccccct tggcggcgac gttgaagatc
     2521 tcgccgcggg cctcgacggc gatccgcttc cccgacggcg aaggggagat gtcggagatc
     2581 gtcttgccgg ccttgagcat gtagggccgg gccgccagct catcgctccg cacctggaag
     2641 gtcagcctct cgtccttgcc cgtcgcggtg tcgaaggcgt gcagatagcc gtcgtgctcg
     2701 tagacgatgg tcttgccgtc cgagctgggc catttgatat ccgcgtccgt atagctcgtg
     2761 agctgcgtgt cctgcttcgt cgccaagtcg tgccggtaga ggttgacggt gccgccggtg
     2821 cggtcgctca cgtaatagat cgagttgccg ttccctgcca ccatcgggaa ccacgagttc
     2881 tcgttgcccc ccggcagctc ggaataggtg ttggacgcga ggtcgtagat cgacaccttg
     2941 ccgacgaggc cgccccggta gcggcgccag cgcaggtagg cgtctgtgcg gttgtaggcg
     3001 atgcgccggc cgtcggggaa gaaggagccg ttggtgatct cacggaccgg cagggcgcgc
     3061 ggcaggccgc ccgcgggctc ggccacccag agccgcggct gcgacggcgt gaagctgccg
     3121 gccggcgagc cgtaggccac cttgccgtcc ggcgtccagc cgacgaggct ctccatgaac
     3181 ggatcgaagg tgatccgccg cggcgctccg ccctccagcg gcatcacgta gatgtcggcg
     3241 ttgccgtcat aggaggccga gaaggcgacg agcttgccgt cgggggagat gcgcgagcgc
     3301 cgctcctcgc cgcggtgggt ggtgagccgc cgggcatggc cgccccggcg gtcggccacc
     3361 cacaggtccg acgaataggt gaacacgacg gtatcgccgt ggatggctgg aaaacggaga
     3421 aggcgcatct cgggcgcctt ctcttgcccc ttttcttgtg aaaaagcggc gctcgatgag
     3481 acgagtcccg cgagcaggat ggcaatcatt gaccgattcg acactatggt cctcccaagg
     3541 ctctgggcat gtacgcagga cggtccccgc ggtttcatgc cggtgaatgt aggaggagcc
     3601 gcgcgatatg tccaatatat ctccgcagat ggatccatac ttcgtgggta tggaaggccc
     3661 gcacttgtcc gggcactccc ccggctgata gcgtcgcgtc caccattgat agggagagac
     3721 ccgatgctgg cacgtccgca ggcccgagcc gccgtcctgt ccgtcctcgc cgccctcgcc
     3781 atcccgggga cggtccttct gggggcgccg ccgcccgcgt ccgcccagac ggacctccag
     3841 cgcgaggtcg accgccgggc gacagaggtg ctgcccaagg tgatcgcttg gcgccgggac
     3901 atccatcaga acccggagct gggcaaccgc gagacgcgga cgtcggccct ggtggcggac
     3961 cacctccgca agctcgggta cgaggtgcgg accaacgtcg cccacaccgg cgtcgtcggc
     4021 atcctgcgcg gcggcaagcc cggaccggtg gtggccctgc gggccgacat ggacgcgctg
     4081 ccggtcaccg agcaggcaga cgtccccttc gcctccaagg tgcgcaccac ctacaacggc
     4141 caggaggtcg gggtgatgca cgcctgcggt cacgacgtcc acaccgccgt gctgatgggc
     4201 gcggcggagg cgctcgccgg ggtgcggcag gagctgccgg gcacggtcaa gctgatcttc
     4261 cagcccgccg aggagggggt gccgcccggc gaggaggggg gcgcgaagat gatgatggcc
     4321 cagggcgtgc tcgccgaccc ggcgccgcag gcgatcttcg ccctccacac cacgacgctc
     4381 cccggcggta ccctggccta caaggccggc tcgatctacg ccagcgccga cgtgctgaag
     4441 atcgtcgtcc gcggccggca gacgcacgcc gccatgccct ggcaggggat cgacccggtg
     4501 atcctctcgg cgcagatcat cctggcgctg cagaccatcc ccagccggca gctcgacccc
     4561 ttcctgccca acgtcgtgac catctccacc gtccacggcg gcgtgcgcaa caacatcctg
     4621 cccgacgagg tggtgatgga ggggaccatt cgctcgctga acccggcgct gcacgaagac
     4681 ttcctggcgc gcatccggcg cacggcggag tcgatcgcca ccaacgccgg cgccacggcc
     4741 gaggtgacga tcacccccta cgccccggtc accgtcaacg acgccgccct gacccgccgc
     4801 atggagccga cgctgcagcg catcgccggc acgagcctct ccggcacccc cggctcgatg
     4861 gcggccgagg acttctcctt cttcgcccag gaggtccccg gcctctacgt cttcctcggc
     4921 atcaacccgc cgggcgtcga gccgggcgag gccgccccga accactctcc ccgcttcttc
     4981 gtccacgagg actcgatggt ggtgggcatc cgcacgatgg cggggctggc ggtggattat
     5041 ttgatgggag agtgaggggg caccgccgcc gggtcactac gctccccggc ggcggtgagg
     5101 ccagacgtca gacttcgtga tactccagaa tgcctcggtc ggggtcctgg aggacgcgga
     5161 ggacggtcca gagacgggac ggcgggactt cgccttcctt ctcctcgagc aggcgccaag
     5221 tgatgaacca gcgtccatag agttgatgga cggtcaggcg cgcctgctcc ggagtgtagg
     5281 ccggcggctg ccagctctcc ggatccgcca cggccttgtc cttctcctcg ggcatcgggg
     5341 tgaagtgggt ggccaccgcg gcgtcataca acgcgcgccg ggtgtcctcc gtctcctgcg
     5401 tcaggtcgac agcagacttg gacggctcgg acggaggggt cggtgtacga ttgtcacggg
     5461 tgttcatgga gttcatagct cctttgggca cctaggctcc gggtgtaagg ggtccagcct
     5521 tcactcggag ccgctcggtg ctgattcccg cggcggagag attccaccac ggggccagca
     5581 aggtacagcg acaacaacga caggcatcag tatagcgcaa tgtcttgggc gaagacaagc
     5641 ttccagcttc cttcgcctgg ttcaaatctc cccgacaccc cctccccgac ctccccaggg
     5701 ggaaagtttc ccgccgcagg aagagaaccg ccggcctgtt cgccagcttg tccgcccccc
     5761 tgcccgccgg cggtttctcg gggaactgct ccgggaccta tctgatggcc gaagcgggcg
     5821 gcgcgacgtc cctgtggacg ctggaggccg acgggacgtt cttcgccacc agctcgctcc
     5881 aggcactgct caagttttcc gatcagcagg ggagctggga aaaggtcggc aacgacggca
     5941 tcaagggcgt ggtgctcgcc ctcgtgttcg atgagaacga cgcgctcctc aacgtcagcc
     6001 gcgtggacat ctccctgcac acggtgggca gagggtgcga caacatcgcc ggctcgctgg
     6061 aggtgcgcga gttcaccggc gccgaggatc ctctcgaccc cgccaccgat accggcgaac
     6121 ccatcgccac cgacaccgtc accggccgcc gcgtgaaggc gaaccggtag cgcgcaaggg
     6181 accgggagcg catcgatctc caggaagctc gcgctttcat catcccgagc gcctccgtct
     6241 ccgtacccgc ggagacgggg ctcatttctc ccggggcagc cgttgcccca cgagagacgt
     6301 ccgccatgcc ccggaaaacg ttcgtcctac ccggggtacg gccgtcatct cccgggggag
     6361 gacggccgta cccacggggg gatcgccgta cccgcgggta tggcgttcat ccctggggta
     6421 gcacggccgt acccgcgggg atgaggaccg gctgcccggg gatggccttg tttcatcggg
     6481 tagggccttc gtctcccggg gtatggcctt ccttttacgt ggagggtgga cgttcctcga
     6541 aggacggcct ccatttcccg gatggcagac ggccgttgcc gcagggatgt cgccattccg
     6601 tgctgattcg ttgccgaagc cgcgcggccc ggcctcctcc ccagggagga tgtacaccat
     6661 ctctccaggt ccgctaccgc catcgccgcc accggcgcgg acatcttcga ggtgggtcac
     6721 gactacgttc tcgtcgaagc cacgaggcgc gaagccaggg ccttgaagcg gctgcgcctg
     6781 aagctcgcgc ggttcgggac gcaggacgaa ttccgcccag cccaacgccg gcacgaccgc
     6841 cacttcgcct ccggccggtg ggtgaccgtc ccggccggtc gttgccggga cgtcgcatga
     6901 tctctccctc ccgcccaata cgagccccca gggttggcta ccggctcatc gagaagcaag
     6961 gctctcatcc cttggcagcc tgagggccct gataggctat tgggcgatga ggagccacgg
     7021 acgatgatcg atcaggctgc agagacgatc ctcgaaacgg gccggagcgg catgccgccg
     7081 cggccggcct tcgacgtgcg gcggctgggc ccggcgcgcc ttccctcgcc gctctccggg
     7141 cggcgcgagc ggttcatcgg cgacggcgag cgggtgctcg ccaccaccga cgcggtgcgg
     7201 atcgccgact acctggaggg ggagggggcg ctcccctgct tcgagcgcgc cgggccgcgc
     7261 cgggagctgt tcttcgaccc gcggaccctc ggcgccggca tcgtgacctg cggcgggctc
     7321 tgcccgggcc tcaacaacgt catccgctcg atcgtcctca acctctacta cgcctatggc
     7381 gtgcggcgga tcctcggcta ccgccgtggc tacctggggc tgtcgcgccg gcgccccgag
     7441 gagccgctgg agctcgggcc tgcccaggtg gagagcatcc acgagcgcgg cgggacgatc
     7501 ctcggcacct cccgcggccc ccaggacgtc ggcgagatga tcgacaccct gcagcgggac
     7561 ggcgtccaga tcctcttcac cctcggcggc gacggcacgc tacgcggcgc cgcggcgctc
     7621 gcccgcgaga tcgagcggcg ggggctcgcg atcagcgtca tcggcgtccc caagacgatc
     7681 gacaacgaca tcgcctgggt ggaccgcagc ttcggcttca ccactgccat cgaggagggg
     7741 aaccgggcga tccacgccgc ccatgccgag gcgcaggcgg cgtggaacgg catcggcatg
     7801 gtcaagctca tgggccgcca ctccggcttc atcgccgccc acgccaccct gtcgaacaac
     7861 gacgtcaact tctgtctggt gccggaggtc ccgttcagcc tccacgggca tggcggcttc
     7921 ctggaggcgc tggagcagcg tctccaggag cggcgccatg ccgtgatcgt ggtggcggag
     7981 ggggcggggc aggagctgct gcggcgggaa gggcaggtgg agcgcgacgc ctcgggcaac
     8041 gtccgcctgc tcgacgtcgg ggtctatctg cgcgaccgca tcaccgagca cttctcgtcc
     8101 cagggaatgg acgtcaccat caagtacatc gacccgagct acatcatccg ctcgcagccg
     8161 gccaacagct cggacgccga gctctgcctg ttcatgggcg agcacgcggt gcacgcgggc
     8221 ctcgccggcc gcaccgagat ggtggtcggc taccgcaacc aacagttcat ccacctcccc
     8281 atccccctcg ccgtggcgcg ccgcaagagc ctcgaccgcc gcggcaagat ctgggaaggg
     8341 gtcctcggca ccaccggcca gccggtctcg atgctcggcg acagcgcgat ctagccctcc
     8401 gcgccccacg gggccggcgg ctcgccccgc ggcgccgacg gccggcccca cgaagccgat
     8461 ggcgcgcccc gcggggcgaa cgacccggcc gacggggcca agagctggcc ccaaggggcg
     8521 gacggctcgt cccatggagc cggaggctgg cccccagggg cggaggacct cactggccgg
     8581 gccggtggcc ggctccgcgg ggccgccggc aggcggcatg gggcggaggg ctggccccac
     8641 agggcggacg gcgcgccccg cggggcagag gcctccgtta gaaccgccgc ccgagcgaca
     8701 ggtagggctc gtcgtaccgg cgggccgact ccccccacac ccactggttg tagccggcct
     8761 cgacgctcca gcggccggcg ctgtcgaggg gggcgatgat cccgacgccg atgcggccca
     8821 tgctggcgtc gttgaagttg aagtccggcc gcgagccgaa gcggtcgtcc ggctggcgcg
     8881 gcgagtcgtt gcccaggctg ccgatgtaga cggccgcgac cttcccctgc agcttccaga
     8941 gcggcagcgg ccagccgagc tcgccccaga ccgggacctc gtccgccggc gccccctcgc
     9001 gccaggtgta gcccgcctgc aggttgaacc agccgtgccc ctcgcggaac gggcggctca
     9061 ccaggtacga cagcgtcagg tcgtgcttga gcaccccgcg ccactcgggg ctcacgtcgc
     9121 ggatgttgcc gttgctgtca tagagatagc gcgagtaggg cccctcgagg tcgtagaagt
     9181 agggggtgcg gtaggtgagg gcgagcgcca tcggcaggtc cccctgcacg agggcgtact
     9241 tgaggccgaa ccaggcgtcc gacgggccgg tgttcttctc caggtggtcg ccggggccct
     9301 ccaggccgtg gagataggtc aacaggaagg tggtcgagag ccgcgggagc aatcccacct
     9361 cgcccgagag gtagccgtag cggaaatcgt ggtaatgccg cttgacctcg ccgtcgacca
     9421 ccgtggtgtt ggtgaaggaa tcgccaaacg cgttccagga cgtggacgcc ttcttctggc
     9481 tgtaccccag ttggacgtcc ccctgccccg gctccggcac ccaagccccg cccgccaggg
     9541 cggcgcccgg aacccccgcc aacaccaaca gcaccgcacc gacaatccga tccccacgca
     9601 tacggctcct ttcgcgctcc aggaaacagt gtgagcgtct tacctctccc ggtgcgcctc
     9661 gcctgcccgg cgtgaccggg agcggctctt tgcggcatga tgcgccggtg ccccgccacc
     9721 cgctccttct gatggtgctc tcctcggcct gcttcgcctt catggcgctg gcggcgaagc
     9781 tcgcggccgg gggactgtcc ggcggccagg tggcgttcgg ccgcttcgcc ttcatgctgc
     9841 tgccggtggt cctggtgccg ccgctggcgc gcaaggcggt cacctttcag cggctggacc
     9901 tgctcttcta ccgcggcctg ttcggcggct cggcggtgct cctctacttc ttcgccctgg
     9961 agcacatgcc ggtggggatc gcgacgctgc tcaactactc gtcgccgatc ttcagcgtcg
    10021 ccttcgccgc cctcttcctc ggcgagcgtg tggacgggcg gctgctcctc cccctgatcg
    10081 cggcgctgtc cggcatggtg ctggccgccg gaggcggcgg cgggctgggg gggatccgct
    10141 tcgggctctg ggaggcgctg gggatggcct cggcggtgct ctcgggcgcc gcggtgacgg
    10201 cgatccgcgc cgcccgccgc accgaggggt cgtgggcgat ctacggcagc ttcacgatct
    10261 tcggcctgct cgccaccgcc cccttcggcg cccgggggct cggcgccgcc acctcccgcg
    10321 agtggctgct gctggcggcg gtgggggcgt tctccatcgt cgcccagctc ctcatgacct
    10381 atgcgtaccg ctgggtgacc aacctgcagg ccggggtggt caaccagctc acggtggtcc
    10441 tcagcctgct cctcggcgtc gtcttcctgg gggaccgcct ggcgccggtc cagatcgtcg
    10501 gcagcctgct caccctcgcc ggggtggtgg gggtggtggc ggtccaatcc actccgcggg
    10561 ccgtcgaatg aggatcgaac gaggagggcg cgcttccctg cggccccgaa accggaaata
    10621 atgaccgcgg ctgtacctgg gcaggatctc tagcggagaa gacgccatga gactggcact
    10681 ggtcaacgtc aagggtggtg tcggcaagac gacgacggcg gtgaacctgg cggcagcctt
    10741 cgcccgcggc ggcctgcggg tgctgctggt ggacctcgac ccgcagggct cggcgagcct
    10801 ctccctcggc gtcggccgcg cggagaccca ggaccagccg acggtcgccg agctgctcac
    10861 cggcgaggcg gaggccgccg cggcggtgcg ccagaccggc gcggcgggga tcgacctgat
    10921 cccgtcggcg ctgcggctgg ccgccatgga cctcgccctc gcccgggagg cggagccggg
    10981 aaagctcctc aagaaggccc tcgcccccct gcgccggcgc tacgacgtga cggtgatgga
    11041 ctgcccgccg gggctgtcga tcctgagcgt caacggcctg acggcggcgc aggccttcat
    11101 cgtcccggtg gcgccgcacc acctggacat ggacgcgctg gccggcttct tcgagctctt
    11161 ggaaatggag cgtggccggt tcggccgcaa gaccgagctg ctggggatcg tcctcaccat
    11221 ggtcgaccac cgcacccggg tgaccgacga ggtggtggcg gcgatccgca agagctacgg
    11281 caagcaggtc ttcaagtccg agatcccgat caacgtccgg ctggccgagg cgcccggcca
    11341 cggccgcacc atcttcgact tcgagagctg gtccaccggc gccctcgcct acagccacct
    11401 cggcggcgag gtgctgcgcc gcgcccgcaa ggcggggctg ctggagagct gacccccggc
    11461 agccccgtcc ggccgtcagg ccgctgtctc aggcgatcgc ggtcgccgcc ggcgcggcct
    11521 cggccggctt gcggcgcagg atggcggcgg agatcagcga gacgatcagc cccaccggga
    11581 agacctccag gagggtgatg gcggagttga agaagatgtt gtcgtagtac ttcgccatga
    11641 aggccatctg cccctccatc ttctggatgg cggccgcgct ctcgccctcc gcccgcatct
    11701 tcgccagcgc gcgagtggtg tactcgtcga ggaagtcggg gaagaagttg aagtacgcga
    11761 tctcccaggt gatgacgtac atggcgcagg tgatcagggt gatgagcagg ccgacctgga
    11821 acgccttgcc gaacccgatg gcgccgccgg cgacggtgtc acggtacgac cggacgccga
    11881 agaagaccag caggaacgac aggaccatgg cgctgtagcc gatgacctcg ctgccttcga
    11941 ggtcgatggc ctcgttcatg gtgaccgccc acaacaccga cgacaaagcc accaggatca
    12001 cacccgacgc gagcccgaag cgaagcacga ttctcttcat tgcgattccc tccttggttg
    12061 gcccgcaagg tagcgccgcg gcgccgcgcc ggtcgtccta cggtcgggtg attttcgggc
    12121 ctggaccccg gaatcatact ttcgttgacc cccgggccgg ctcgcggagg ccgggggatc
    12181 aggggatgag gccgagctct ttccccctct ggacggcctg gacccggcgg ttgaccccga
    12241 gcttgccgaa caggcgggag gagtgggtct tgacggtgtt ctcggagacg aagaggcgct
    12301 cgccgatctc gcggttgctc aacccctcgg cgatcagccc gaggatctcg tgctcccgcg
    12361 gggtgatgcc gagctccgcg agcttgccgg ggtcaagcac gaacggcctc tcctcgcgga
    12421 cccgcacctc caccacctcc cggaccacca cctctcttgg ctcggccggc tcagccgtcc
    12481 tctccggctg ccgcggccgg ctccaccgca gccccagata gatgcctacg gcggtgaaga
    12541 tcaccgccac caggccgcca tagatctcgg tcgggtaggc gcggacgaag tgcttgtact
    12601 ccaggacctt gagcagcgtg atgagcacgc cgcccaccaa gccgtagagg atgacgcgtc
    12661 tcacggccgg agagcatagc gcgtccccgg acgcagaaag gccggcgtca ggggcgccgg
    12721 ccttcggttc agcggcagct ccggatcaga gctcgactgt cgtggtcgtg cagccggggc
    12781 cgtagaagat ggtccagccg gacggcgcgg cacacggggt cgagtactgg cagcagcgct
    12841 ccttgtcggg gctctgcgcc cagacgatga cctggctgca ggagccgctg tactgctgag
    12901 gcggcgggca gtgatgattg gagctcgcgg ttcctacctg agtggccgcg acgagagcga
    12961 acgcggccga agacaaaaca aacctcttca acgacataga ttctcctgaa ggactctcgt
    13021 ccttggtctc tggtttcgtt gtcccggggc cccttcgcga aatcaatgta gctaaatttc
    13081 tatggaagtc aactcttggg cggctccacc gggagctcga ttccggccgc gagccagtcg
    13141 agggacagcc aaggagggga ggcggcgaag tcgtaggtct ccaggatgag gaccggcgtg
    13201 ccgggggtga gcacgtggcg gcggctctcg tcgagcgtcc acccctggcc ccagctgtag
    13261 agccagacgg cgtcgcgctc gaggagccgg atgcaggcgt ggctcgcggg gcggccgggc
    13321 agctcgagct ggtggaaggc caagccgcgc cgggtgttga agttgtagta ccacggcatg
    13381 taccagtccg ggttatcggt gctgcgccgc cccttgctcc gccagttcag gtggaagagc
    13441 ccggcgggcg tcgggttctt ctcccgcccc gagctgaccg gcccccagcg caccaggagc
    13501 ccggcttcgt aggcgccgaa gacctgcgac ggctggtgga cgacgagtgc cttggggtgc
    13561 tgcgccgccc agtcccagct cagcggcagc gggctgtaga gcagctcgtc cggcagccac
    13621 tcctcgggga cgacgagcgt cttggcccgc gccagatggg ggacgtcgcg gcggttgagc
    13681 ttctcgagga gcgccagttg ctccgggttg aacagagccc gcagctcctt ctcccctccg
    13741 gtgaggggca tggacctgta ggtccgctcc cgggtggggg tcccggccgg cgcgaccgcg
    13801 gcagggactt ctcccggcac ctcgacgatg accggcggga tgggcggagt ggggacctcc
    13861 ggcggagtct gtgttgtctg tcccgcggtt ttcgaggggg cgatacccgc gagacagagc
    13921 agcgcggcgg cggtccacgt ccttccacaa cccatgtcgt tcctctctga cgccgcgaga
    13981 cgcggagcct gtcgcgccga gtgcaggcaa ggaacgttcc agttcggcag ccgcccggag
    14041 ggggccccct ccgggcggcg caggggtcgg tcgaccggcg ctcaggtgcg gcaggtgcag
    14101 ggggagcgcc ggggctcgtt cgggcagacc tggttcaccg cgtggcagaa gcccggctgg
    14161 ccgctcgccg tggtgcactg ggagagagtc ggcttcccgg cgcagcgggt gtcactgcac
    14221 aggtcgccgc agtcggggta gatcactttg aacaccggct tgttctcgtc cggcttggcg
    14281 accagcgaag agggctcgag ctggcagggc gcctgctccg agagctccgc caggaagagg
    14341 gcgttcgagg tggaagccgg ggcggcctgc gacggcagcg ccatgccgag cgccagcacc
    14401 agacagagac cgagcaggga ccaacgagaa atacgagaca tgcaagctcc tttcgacata
    14461 cccggacgac atcgtccgcg taaaaccagc cggggtatcc ctcccaaccc agcccacgaa
    14521 agagatacgc catgacaaag ctattcttac ttgcgaatgt ccatctgcca acggcgggaa
    14581 ggatctccgg atctatactt cccgtcgcca aggaggagag agcgtggcta ctgtgaccgg
    14641 tccttcagcc cagaccctgc cggacgacct ggcggccctg cgccggcagg tcgaggacaa
    14701 ccgccggacc gccgaggatc tcgtccgcgg cctgagcgag gcgcagctcc agtggcggcc
    14761 ggacgagcgc tcctggagca tcgcccagtg cctcgatcac ctggcgatcg gcgaccgcgc
    14821 ctacctcgag gccatgagcc cggcggtggc cgaggcgcgg cgccggaatt caaggcgccg
    14881 cggcccgatc cagccgggct ggttcgcgcg ctggttcatc caatccatgg agccgccgcc
    14941 gcgccgcaag ctgccggcgc cgcgcaagat cgtcccgtcc ctcggcaaga gcggcgacga
    15001 ggtgaagcgc gactacttcg ccgcccaggc cggggtcctg gccctcctgg accaggcggc
    15061 ggcggtcgac ctcaaccgca cgcgcttcgt caatcccttc ctgccgctgc tccgcttcac
    15121 cgtcggcacc ggcttcctgg tcagcgtcgc ccacgggcgc cggcacctct ggcaggccga
    15181 gcggatccgg cagcgcccgg ggttcccccc atgacagggg aggaggcgag tgaaatgacg
    15241 gacgagcccc agcggcggcg atgggcgcgg gtggtgctgc gggtggccgt ggcgctcctc
    15301 ctgatcatcc acggcgtggc gcgcgcctcg ctgggcatcg tggacgactt cggaggcttc
    15361 ctggcgagcc tcggcctccc cttcggaacg gccgtggcct ggctcctcac cgtggtggag
    15421 atcgccggcg gcctcgccct ggcggcggga ttcctggtgc tgccgctctg cgtctggttc
    15481 gccgtcgagc tcgccaccgg gatcgccctc gtccacgccc cgaacggctg gttcgtggtc
    15541 ggcgccggcc gcaacggcgt ggagttctcc gtcctgctga tcgtctgcct ggtggtgatc
    15601 accctggagc acccgggcag gagacggacc tccccctaaa tcggtcccct acgccttcgc
    15661 ctcgtaccag ctccgccccc atccgacgtc caccaccagc ggcaccttga ggtgggccac
    15721 gccttccatc tcctggcgga ccggaccggc gatggcttcg gcctgggact cggggacctc
    15781 gaagacgagc tcgtcgtgga cggtcaggag cagccgcgcc tcggggtgct cctggcggag
    15841 acggcagtcg atggcgatca tggccttttt caggagatcg gccgccgtcc cctggatgcg
    15901 ggcgttgatc gccatgcggc gggcgttctc ccgcaggctc cggttcttgc tctggatctc
    15961 cggcagccag cgcacccggc cgtagagggt cgtcaccttg ccctccttct cggcgctcgc
    16021 ctgcgtctcc tccatgtagg tacgcacgcc ggcgaagcgg tcgaggtagg cgcggatgaa
    16081 gtggtccgcc tccttggtgg agatgcccag cgcctggctg aggccgaagg ggctcatccc
    16141 atagaggatg ccgaagttga tcgtcttcgc agcccgcctc tggtcccccg tcaccagctc
    16201 cggggcgatg ccgaagaccg tcgccgcggt cgagcggtgg atgtcctccc ctgccaggaa
    16261 ggcgcggatc agctctttct cctcggcgat gtgggccagg atccgcagct cgatctggct
    16321 gtagtccgcc accagcagga cgttgccggg gctcgcgatg aaggccttgc ggatacgctg
    16381 ccccagctcg gtgcggatcg ggatgttctg caggttcggg ttggtcgacg acagccgccc
    16441 cgtggccgcc accgcctggt tgaagcgggt gtggagacgg ccgtccgggc cgaccagcgt
    16501 cggcagggcg tcgatgtagg tggacttgag cttgatcagc tcccggtagc gcagcaggta
    16561 ctgggcgatg gggtagcccc ggcccgccag ctcttccagc gtctccgcgc cggtcgagta
    16621 gctcttggtc ttctgggtgc gcttgagcac gggaagcttc atcttctcga agaggatcgt
    16681 cccgagctgc gccggcgagt tgaggttgaa gcgctccccc gcctgctggc agatctcctc
    16741 ctccagcgtc tccagttcct ggccaagctc cgccgacatg tcgcgcagga agccggtgtc
    16801 cagcaggatg cccgcctctt cgaggccgag gagcaccggc accagcggcg cctcgatctc
    16861 acggtagacg gtgtcgagcc cggcgtcctc caggtcccgt cgcaccgtgc cggccatgcg
    16921 gcgggcgaag gccacccgct cggcggcgta ctcggcgagc cgcgagtccc cctgaggcgg
    16981 ctcctggccc ttgtcccaac ccgactcctt ggcgctgatc gccttgcgcg agagccgctc
    17041 cagcgccatc tcgtccaggc tgtggccgtg gagcgagggc ttgatgaggt acgaggccag
//
LOCUS       DASPJI010000582         4643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_37666, whole genome shotgun sequence.
ACCESSION   DASPJI010000582 DASPJI010000000
VERSION     DASPJI010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37666"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VEL74_16305"
     CDS             <1..1138
                     /locus_tag="VEL74_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome D1"
                     /protein_id="HYG64139.1"
                     /translation="ADGTVSVVDVARLAKVRDVAVGSRPTSIAWSIAARAAYSVQTGS
                     GTIAAVRADAAEPAARIQADPGLGRVRFAPGGRLGFVVHTEKKLVHIVDAASNRIVQT
                     ADVEAEPDLVTFSDELAYVRHRGSETVLMIPLDVVGQEGRPVPIVDFPGGQNPPGKTP
                     MPSVADGIVQAPGAAAVLVANPMDKVIYFYKEGMAAPMGHFRNYGRIPRAVMVLDRSL
                     RETAPGSYETTVQLGEAGDYEVALLLDTPRLTHCFPVTIAADPALAARREKSALGVEI
                     LDQPSTVTAGEELAVRFRLTDSAKGSARTGLRDVRVLTFLSPGIWQQRHWASDQGEGI
                     YEIRFQPPEPGLYFVFLEVASAGMTFQKSPFLVLQATAGGEPKS"
     gene            1135..2298
                     /locus_tag="VEL74_16310"
     CDS             1135..2298
                     /locus_tag="VEL74_16310"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HYG64140.1"
                     /translation="MIQTTPPAVRAALLALSLTTVPVFGHEHGAHQHHAAPETAKPAP
                     ALPAPERIEGLEIPDVTLLDQEGRPVRFYSDLIKGRVVAVNFVFTSCTAICPPMGGIF
                     GQVRQQLGSRAGQDVHLISVSVDPGTDTPARMKEWGARFGAGPGWTLVTGPRAEIVQV
                     LKAFGAYTADINAHTPQLVLGDDSRGEWTRAYGLAPPAKVMELIDKVAPRPEARAEAP
                     AEPAAERPAAHHYFGDIKLVDQDGRERRLYSDLMAGKTVVIDVMFTTCNGVCPVLSRN
                     LQRVQEALGDRVGKDVHLISISVDPETDTPARLKEYAARYKAGPGWYFLTGPKPDVDA
                     ALRKLGQWVPNPEAHQNLILIGNDRTGLWKKALGVAEATEIITVVESVVNDRG"
     gene            2302..3903
                     /locus_tag="VEL74_16315"
     CDS             2302..3903
                     /locus_tag="VEL74_16315"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYG64141.1"
                     /translation="MSRIAVTCLATVATVALAAAMPARGGTPAASLTAAEERGRTIYR
                     SGVSPRGTPIVAVTAEGAVELPAASMPCSGCHGRDGRGSREGGVAPSDLTWGSLTKPY
                     GGNHPGGRTHPPYTARLLKRAISLGLDPAGNPLHVVMPRYRMSQEDMEDLTAYIQRLG
                     HEPVPGLSGDKIRVGTLLPPGPAAEPVRRLLDAYFTEVNARGGVFGRRLELLPMTASG
                     AAGAVGDRRVRIEEALDREPVFALAGAFLGGADKELAALFQERELPVVGAIGSGPVPG
                     EASPPVLFHLLSGLEGQSRALVQFAAQDAGEGKGARPKLASLAAEEPALTALVDAVER
                     EAKSRSWVLAGSSRYRPGALDAAATARALRDSGADLLFFAGPGADQGALLAAADALGW
                     RPRVLSPGALGGSGLFAAPPAFHRRLFLSFPTLPQDQSPAALADYQRLAAGAGLPRGA
                     LPAQLSALAAARLLVAMLERAGRDLTREALITQLEKLDHFETGLLPPLTYGPGRRIGA
                     RGAYVVAVDLEKKTFTPASGWIGVD"
     gene            3963..>4643
                     /locus_tag="VEL74_16320"
     CDS             3963..>4643
                     /locus_tag="VEL74_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HYG64142.1"
                     /translation="MSSVHRRRFRLRQLPRLAALLLLLAAPGAATAQSCTRTLMADVV
                     AIDQVWFWNRLGAVQPQGMMYALRRDVVPISGNSLTAGNARLRPDKRPRPMVLRMNVG
                     DCLRIQFQNLLDPTRRDQDQPNTRHASIHVIGLQLFGHINEDGSYVGQNASSLVSPGQ
                     SAIYTYYAEREGEHVLYSAGTTTGGQGNGGQIAAGLFGAVVVEPAGARWYRSQVSRAD
                     MDLVTSSTN"
BASE COUNT          654 a         1767 c         1597 g          625 t
ORIGIN      
        1 ggccgacggc acggtctcgg tggtcgacgt ggcgcggctc gccaaggtgc gggacgtggc
       61 ggtcggctcc cggcccacct ccatcgcctg gtccatcgcg gcgcgggccg cctactcggt
      121 gcagaccggc agcggcacca tcgccgcggt ccgcgccgac gcggcggagc cggcggcccg
      181 catccaggcg gaccccggcc tcggccgcgt ccgcttcgcc cccggcggcc ggctcggctt
      241 cgtggtccac acggagaaga agctggtgca catcgtcgac gccgccagca accgcatcgt
      301 gcagacggcc gacgtggagg ccgagcccga cctggtgacg ttcagcgacg agctggccta
      361 tgtccgccac cgcggcagcg agaccgtgct gatgatcccg ctcgacgtgg tgggccagga
      421 ggggcggccg gtgcccatcg tcgacttccc cggcggccag aacccgcccg gcaagacccc
      481 catgccctcg gtcgccgacg gcatcgtgca ggcgccgggc gccgcggcgg tgctggtcgc
      541 caacccaatg gacaaggtca tctacttcta caaggagggg atggccgcgc ccatggggca
      601 cttccggaac tacggccgca tcccccgcgc cgtgatggtc ctggaccgga gcctgcgcga
      661 gaccgcgccg ggctcctacg agaccacagt ccagctcggc gaggccggcg actacgaggt
      721 cgccctgctg ctcgataccc cacgcctcac ccactgcttc ccggtcacca tcgccgcgga
      781 cccggccctg gccgcgcggc gcgagaagtc ggcgctcggg gtggagatcc tggaccagcc
      841 gtccacggtg accgccggcg aggagctggc ggtccgcttc cggctcaccg actccgccaa
      901 gggtagtgcc cggaccggcc tgcgggacgt gcgcgtgctc accttcctct cccccggcat
      961 ctggcagcag cgccactggg cgagcgacca gggcgagggg atctacgaga tccgttttca
     1021 accgcccgag cccggcctct acttcgtctt cctcgaagtg gcctcggccg ggatgacgtt
     1081 ccagaagtca ccgttcctgg tgctccaggc gacggcagga ggagaaccga agtcatgatc
     1141 cagaccaccc cgcccgccgt ccgggcggcc ctgctcgcac tctccctcac gaccgtcccg
     1201 gttttcggcc acgagcatgg cgcgcaccag catcacgccg cgccggagac cgcgaaaccg
     1261 gcgccggcgc tcccggcccc cgaacggatc gagggcctcg agatccccga cgtgacgctc
     1321 ctcgaccagg aggggcggcc ggtccgcttc tacagcgatc tgatcaaagg gcgggtggtg
     1381 gcggtcaact tcgtgttcac ctcctgcacg gccatctgcc cgcccatggg aggcatcttc
     1441 gggcaggtcc ggcagcagct cggcagccgc gccggccagg acgtccacct gatctcggtg
     1501 tcggtcgacc cgggcaccga cacacccgcc aggatgaagg agtggggcgc gcgcttcggc
     1561 gccggccccg gctggacgct ggtgaccggc ccccgggccg agatcgtcca ggtgctcaag
     1621 gccttcggcg cctataccgc cgacatcaac gcccacacgc cgcagctcgt cctcggcgac
     1681 gacagccggg gagagtggac ccgcgcctac gggctcgccc cgccggccaa ggtgatggag
     1741 ctgatcgaca aggtggctcc ccggccggag gcccgggccg aggcgcccgc cgagccggca
     1801 gccgagcgtc cggcggccca ccactacttc ggcgacatca agctggtgga tcaggacggc
     1861 cgcgagcggc ggctctacag cgacctcatg gccggcaaga ccgtggtgat cgatgtgatg
     1921 ttcaccacct gcaacggcgt ctgcccggtc ctctcccgca acctgcagcg cgtccaggag
     1981 gccctgggcg accgggtggg caaggacgtc cacctgatct ccatctcggt ggacccggag
     2041 accgatacgc cggccaggct caaggagtac gcggcccgct acaaggccgg tcccggctgg
     2101 tacttcctga ccggccccaa gccggacgtg gacgccgccc tgcgcaagct cgggcagtgg
     2161 gtgccgaatc ccgaggcgca ccagaacctc atcctgatcg gcaacgaccg caccggcctg
     2221 tggaagaagg cgctcggggt cgccgaagcc accgagatca tcacggtggt ggagagcgtc
     2281 gtcaacgatc gcggctgagc catgagcagg atcgccgtga cctgtctcgc caccgtcgcc
     2341 acggtggccc tggccgccgc catgcccgcc cggggcggca ccccggccgc ctccctcacc
     2401 gccgcggagg agcggggccg cacaatctat cgctcggggg tgagcccccg cggcacgccc
     2461 atcgtggccg tgaccgccga gggcgcggtg gagctccccg ccgcctcgat gccctgctcc
     2521 ggctgccacg gccgcgacgg ccggggcagc cgcgaggggg gcgtcgcccc gtcggacctc
     2581 acctggggat cgctgaccaa gccctacggc ggcaaccacc ccggcggccg cacccatccc
     2641 ccctatacgg cgcggctgct caagcgggcg atctcgctgg ggctcgaccc ggcgggcaac
     2701 ccgctgcacg tggtgatgcc gcgctatcgc atgtcccagg aggacatgga ggacctgacc
     2761 gcctacatcc agcgcctggg gcacgagccc gtgcccgggc tgtccggcga caagatccgc
     2821 gtcggcacgc tgctgccgcc gggcccggcc gcggagccgg tgcgccggct cctggacgcc
     2881 tacttcaccg aggtcaacgc ccgcggtggc gtcttcggcc ggcggctcga gctgctcccc
     2941 atgaccgcct cgggggctgc cggagccgtc ggagaccgac gggtccggat cgaggaggcc
     3001 ctggaccgcg agccggtctt cgcgctggcc ggcgccttcc tgggaggagc cgacaaggag
     3061 ctggccgcgc tcttccagga gcgggagctg ccggtggtgg gagccatcgg ctccgggccg
     3121 gtgccgggag aggcgagccc tcccgtcctc ttccatctcc tctcggggct cgaggggcag
     3181 agccgggccc tggtccagtt cgcggcgcag gatgcgggag agggtaaggg ggcccggccc
     3241 aagctggcct cgctggccgc cgaggagcct gcgctcacgg cgctggtgga cgccgtagag
     3301 cgggaggcga agagccgcag ctgggtgctg gccgggagct cccgctaccg tccgggtgcc
     3361 ctggacgcgg cggccacggc ccgcgccctg cgggactccg gggccgatct cctgttcttc
     3421 gccggcccgg gcgcggatca gggggccctg ctcgccgccg ccgatgccct cggctggcgg
     3481 ccgcgggtgc tctcgcccgg cgccttggga ggctccggcc tcttcgccgc cccgcccgcc
     3541 ttccatcgcc gtctcttcct ctccttcccc accctccctc aggaccagag ccccgcggcc
     3601 ctggcggact accaacggct ggccgccggc gccggcctcc cccgcggcgc cctgccggcc
     3661 cagctctcgg ccctggccgc ggcccggctg ctggtggcga tgctggagcg cgccgggcgt
     3721 gacctcaccc gggaggcact gatcacacag ctcgagaaac tcgatcattt cgagaccggg
     3781 ctcctccctc ctctcaccta tggccccggc cgccggatcg gcgcgcgggg cgcctatgtc
     3841 gtcgcagtgg atctggaaaa gaagaccttc actccggcca gcggctggat cggggtggat
     3901 tgatccgtct cgtctgttcg atctggggcc gcaatcacgc gtccaccttt agaggagact
     3961 tcatgtccag cgtgcatcgt agacgtttcc gactccgcca actcccgagg ctcgccgccc
     4021 tgctgctcct gctcgcggct ccaggagcag ccacggccca gagctgcacc cgcaccctga
     4081 tggccgacgt cgtcgccatc gaccaggtct ggttctggaa ccgcctgggt gccgtccagc
     4141 cccagggcat gatgtacgcc ctgcggcggg acgtggtgcc gatcagcggc aactccctca
     4201 ccgccggcaa cgcccggctg cggccggaca agcggccgcg gccgatggtg ctgcggatga
     4261 acgtgggcga ctgcctgcgg atccagttcc agaacctgct cgacccgacg cggcgggacc
     4321 aggaccagcc gaacacccgg cacgcgtcca tccacgtcat cggcctccag ctcttcggcc
     4381 acatcaatga agacggctcg tacgtcggcc agaacgccag cagcctggtg agccccggcc
     4441 agagcgccat ctacacctac tacgccgagc gcgagggcga gcacgtcctc tacagcgccg
     4501 gcacgaccac cggcggacag gggaacggcg ggcagatcgc cgccggcctg ttcggcgccg
     4561 tcgtggtgga gccggcgggc gcccgctggt accgcagcca ggtgagccgg gccgacatgg
     4621 acctggtcac ctccagcacc aac
//
LOCUS       DASPJI010000583         6970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_207692, whole genome shotgun sequence.
ACCESSION   DASPJI010000583 DASPJI010000000
VERSION     DASPJI010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207692"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VEL74_16325"
     CDS             <1..301
                     /locus_tag="VEL74_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64143.1"
                     /translation="VVPESVGLSDLRRLFDIFRANRLALSSYPSLPYSGSLELIRATA
                     SMQEAGVNLYQRWSDLAAGGVNAHDLQGDHYSIVLGEGAAQLAELLALWLSRSVK"
     gene            375..>6970
                     /locus_tag="VEL74_16330"
     CDS             375..>6970
                     /locus_tag="VEL74_16330"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64144.1"
                     /translation="MRQQREASSPRPRPGTIPVQPRPARSFPLSFAQERLWFLAQLGP
                     ASSDYNIPLALDFRGPLDPADLQASLAEVVRRHEMLRSRFALEEGRPVQIVEPPGDFP
                     LPLVDLAGLGTEIRGEEARRLAAIEARRPFDLAAGHLLRAMLLRLAATEHRLVANMHH
                     IVSDGWSCSVMSREMGQIYRARLSGSTKALPPLAIQYADFAVWQRQILSGAALEQEVE
                     HWRQRLAGLPPVLELPSDRPRPPVRSPRGASLGFELTERASDGLRELARGDGGTLFIT
                     LLSGFFALLRRYTDRDDLCVGSPTAGRRLMETEELIGFFVNTLVLRADLSGDLSFREI
                     LVRVREVVFDAHAHQDLPFERLVAELAPERSLGHTPLFQVSFTFQNVPPAALDLAGLR
                     VAAVEVETGTAKFDLGLTLGDGAGALRGSLDYSLDLFDAPTIQRMSGHLRTLLEGVAA
                     DPARRLSELPLLGPIETQQLVLEWNDTGAWYGVEAGQSLQELISAQALRTPDAVAAVS
                     GDEHLIYRELALRSGSLASQLAAVGVGPDAVVGICAERSLEMIVGLLGILTAGGAYLP
                     LDPSYPQERLSFVLESAGAAAVLVQDHLADRLPPSSLPRLPLATPLPVLPVLPVPLAP
                     TVHTEPENLAYVIYTSGSTGAPKGAMVAHRAIVNRLLWMQEAYGLSPADRVLQKTPFG
                     FDVSVWELFWPLLVGAPLVFARPEGHKDPFYLADLIEEQRVTVLHFVPSMLRAFLEAD
                     QRASCVSVRAVMASGEALPFDLTERFHERMCGELHNLYGPTEAAVDVSSWACVPDDSR
                     RIVPIGRPIDNLRLHVVDRSWQPVPCGVPGELMIGGVGLGRGYLGRPDLTAERFVPDP
                     FTAEEGEAGGRLYRTGDLARHLPMGEIEFLGRLDFQVKIRGFRIELGEIEAALLAHPG
                     VREAAVLLRQDRLGEPALVACVVPDGGPIPAEELRGFLRERLPEAMVPSAFVPLEALP
                     LSSNGKLDRKALLQLAPAAPAGEERSGLAGHAAPGTPAEEILAGIWEEVLRRGQVGVR
                     DSFFDLGGHSLLATQVVSRVREAFGVEMPVRELFEAPTVAGFAARIERRREPGESALP
                     EAPPVVPVGRNRDLPLSFAQQRLWFLEKLEPGSAAYNVPLSVRLAGRLEADVLARGLA
                     EVARRHEALRTTFPERDGRPVQAIAPVLAVPLMLVDLSGLPEREREAEALRRVTAEAR
                     RPFELASGPLIRGTLLRLAAEDHALLVSMHHIVTDGWSMGIFLRELITLYTALSRGRS
                     GELPELPVQYADFVVWQRSWLAGEVLEAQLAYWRQRLTGAPALLELPTDRPRPLVETH
                     HGAALPLALGPSLSRALVALARREGATPFMVLLAGFAALLGRYSGQDDVVVGSPIAGR
                     NRREIEGLIGFFVNTLALRVELSQGLGFLDLLRQVRQTALEAYAHQDIPFERLVEELV
                     TDRGLGHSPLFQATLTVQNTPPVAVEGAGPELEMRLLGVESASAKFELSLDFALAGEE
                     LAGGLEYNTDLFDASTARRLASHFARLLEGALAAPESLLPEILLLAPAERQQLLREWN
                     DTEAAYPAEELCLHELVWRQAERAPDAVAVTLAEEHLSYAELRRRSGWLAHRLRRLGV
                     GPEVPVAIAVERSPEMVVGLLGILQAGGAYVPLDPDYPAERLSYMVEDSAAPLLLTQR
                     HLVERLPESGARVVLLDEAARPEPGLPPEAPSGVLADNLLYVIYTSGSTGRPKGVMVR
                     HRGVVNRLLWAVQDYPITSADRVLQKASFSFDFSVEECFSPLLAGARLVLARPGGQRD
                     SAYLLEAVVEEEITLLHFVPAVLQAFLAEEGLEACHSLRFVFSGADTLTPELRERFEA
                     RIPPGVLLRNQYGPTEISIDVTEWVCRPGEGRFVAPIGRPIGNGLAYVVDSRLEPVPM
                     GIAGELLLGGAGLARGYQGRPDLTADRFVPSPWGREAGARLYRTGDRVRLLPDGNLEF
                     LGRVDHQIKIRGYRIEPGEIEAVLATHPAVHEAVVVAREDRKRGRSLLACVVPAAGAV
                     VVPEELRSFMREQLPDYMVPSAFAVLEVLPLGPSGKVDRKALAQLATIAEEHAEDRLP
                     GGGQAAPRTPSEEILAAIFAEVLRRERVGVHDGFFDFGGHSLLATQVVSRARQAFGVE
                     LPIRDLFEAPTVAGLAERIERLRRAGAGVEAPPILPVPRQGGLPLSFAQQRLWFLHRL
                     APDSAVYNV"
BASE COUNT          866 a         2318 c         2574 g         1212 t
ORIGIN      
        1 tgtggtgccg gagagcgtcg gcctttcgga cctgcgccgg ctgttcgata tcttccgggc
       61 gaaccgcctg gctctctcct cctatccgtc gctgccttac tccggatctc tggagctgat
      121 ccgtgccacg gcgtcgatgc aggaggcagg ggtgaacctt tatcagcgct ggagcgatct
      181 ggccgccggt ggagtcaacg ctcacgacct ccagggcgat cactactcga tcgttctggg
      241 ggagggggcg gcgcaattgg ccgaactcct ggccctctgg ctctccaggt cggtgaagta
      301 gagtacttat ggctgaatct ctcgaccgtt tggcaggcct gtcgccggag aggcgcgcgc
      361 tgttggaggc cctcttgcga cagcagaggg aggcctcctc tcctcgtcca cgcccgggaa
      421 cgattcccgt ccaaccgcgg ccggcccgct ccttccctct ctccttcgcg caggagcggc
      481 tctggttcct ggcccagttg ggtccggcca gctccgacta caacatccct ctggccctcg
      541 acttccgggg accgctggat ccggcggacc tgcaggccag cctggcggag gtggtgcgcc
      601 ggcacgagat gctgcgtagc cggttcgccc tcgaggaggg acggccggtg cagatcgtcg
      661 agccccccgg tgatttccct ctgcctctgg tggatctcgc cgggctcggg acggagatcc
      721 ggggcgagga ggcacgtcgt ctcgccgcca tcgaggcccg ccggcccttc gatctggcgg
      781 cggggcatct actgcgggcg atgctgctgc ggttggccgc caccgagcac cggctggtgg
      841 ccaacatgca tcacatcgtc agcgacggct ggtcgtgcag cgtcatgtcc cgggagatgg
      901 ggcagatcta ccgcgcgcgg ctctccggct cgacaaaggc tcttcctcct ctggccatcc
      961 agtacgcgga cttcgcggtc tggcagcgcc agatcctctc gggagcggct ctggagcagg
     1021 aggtggagca ctggcgacag aggctggccg gcctgccgcc ggtgctggag ctgccatccg
     1081 accgcccccg gccgccggtg cgcagcccac gcggcgcgag tctcggtttc gagctaaccg
     1141 aaagggcctc cgacggtttg cgtgagctgg cccgcggcga tggcggtacg ctcttcatca
     1201 ctctcctgtc gggattcttc gccctcctcc ggcgctacac ggaccgggac gacctttgcg
     1261 tgggctcgcc cacggcgggc cggcgcctca tggagaccga ggagctgatc ggtttcttcg
     1321 tcaacaccct ggtgctgcgg gccgatctgt cgggagacct ctcgtttcgc gagatcctgg
     1381 tgcgggtacg agaggtggtc ttcgacgccc atgcccatca ggacctgcca ttcgagcgcc
     1441 tggtggcgga gctggcgccg gaacggagcc tgggccacac tcccctgttc caggtgagct
     1501 tcaccttcca gaacgtccct cccgcggctc tggatctggc gggtctgcgg gtcgccgccg
     1561 tcgaggtcga gacggggacg gccaagttcg acctggggct gacgctcggg gacggggcgg
     1621 gcgcgctccg gggctccctg gattacagcc tcgacctgtt cgacgccccc acgatccaac
     1681 gtatgtcagg gcacctgcgg accctcctcg aaggggtggc ggccgaccct gcccggcgcc
     1741 tgtcggagct gccgctgctg gggccgatcg aaacccagca attggtgctg gagtggaacg
     1801 acaccggcgc ctggtacggg gtggaggcgg gacagtctct tcaagagctg atcagcgccc
     1861 aggccctgcg gacgccggac gcggtagccg cggtttccgg tgacgagcat ctgatctacc
     1921 gcgagctggc actgcgctcc ggctcgctgg caagccagct ggcggccgtg ggagtcgggc
     1981 ccgacgcggt ggtcggcatc tgcgccgagc ggtcgctgga gatgatcgtg gggctgctcg
     2041 ggatcctgac ggcgggagga gcctatctgc cgctcgaccc ttcctatccc caggagcgcc
     2101 tctcgttcgt actggagagc gcgggcgcgg cggcggtgct cgtccaggat cacctggcgg
     2161 accggctgcc gccatcctct ctccctcgtc tccctctcgc cacgccgctc cctgttctcc
     2221 ctgttctccc tgttcccctt gcgccgacgg tgcacaccga gcccgagaac ctggcctatg
     2281 tcatctacac ctcgggctcg accggcgcac ccaagggagc catggtggcg caccgggcga
     2341 tcgtcaatcg cctgctctgg atgcaggagg cttatggcct gagcccggcc gaccgggttt
     2401 tgcagaagac gcccttcggt tttgacgtat cggtgtggga gctcttctgg cctctactcg
     2461 tgggggcgcc tctggtcttc gcccggcccg aggggcacaa ggatccgttc tatctggccg
     2521 atctgatcga ggagcagcgg gtgacggtgc tgcacttcgt cccctccatg ctgagggcct
     2581 tcctggaagc ggaccagaga gcgtcctgtg tctcggtgcg ggcggtgatg gctagcggcg
     2641 aggcgctgcc gttcgatctg acggaacgct tccacgagcg gatgtgcggg gagctgcaca
     2701 acctctacgg tccgaccgag gcggcggtgg acgtcagctc ttgggcctgt gtgccggacg
     2761 acagtcgccg gatcgtcccc atcggccgcc cgatcgacaa cctgcgtctg cacgtggtcg
     2821 accggagctg gcagcccgtg ccgtgcgggg ttcccggcga gctgatgatc ggcggtgtgg
     2881 gtctgggccg gggctatctc gggaggccgg acctgacggc ggagcggttc gtcccggacc
     2941 cgttcactgc tgaagagggg gaagccggag ggcgcctgta ccggacgggt gatctggccc
     3001 ggcacctgcc gatgggagag atcgagttcc tgggacgtct cgacttccag gtcaagatcc
     3061 gcggcttccg catcgagctc ggagagatcg aggcggcgct gctggcccat ccgggggtgc
     3121 gggaggcggc ggtgttgctg cggcaggatc ggctcgggga gccggctctg gtggcctgcg
     3181 tggtcccgga cggcgggccc atcccggccg aggagctgcg cggtttcctg cgcgaacggc
     3241 tgcccgaagc catggtgccc tcggcgttcg tgccgctgga ggcgctgccg ctcagctcca
     3301 acggcaagct ggaccgtaag gcgctgttgc agctggctcc ggccgcgccg gcgggggagg
     3361 agcgttccgg gctggccggc catgccgcgc cgggcacgcc ggcggaggag atcctggccg
     3421 ggatctggga ggaggtgctg cggcgcggtc aggtgggagt gcgcgacagc ttcttcgatc
     3481 tgggagggca ctccctgctg gccacccagg tggtctcccg ggtccgcgag gcgtttgggg
     3541 tggagatgcc cgtgcgcgag ctgttcgagg cccccaccgt cgcggggttc gccgccagga
     3601 tcgagcgccg gcgcgagccc ggcgagagcg ccctgcccga ggctccgcct gtcgtgccgg
     3661 tgggacggaa tcgcgacctg ccgctctcct ttgcgcagca gcgcctctgg ttcctggaga
     3721 agctcgagcc ggggagcgcg gcctacaacg tcccgctttc ggtgcggctg gcggggcggc
     3781 tcgaggccga tgtgctcgcc cgcggcctcg ccgaggtggc gcgccgtcat gaggcgttgc
     3841 gcaccacgtt ccccgagcgg gatggacgtc ctgtgcaggc gatcgccccg gtgctggcgg
     3901 tccccctgat gctcgtggac ctttcgggac tgccggagag ggagcgcgaa gccgaggctc
     3961 tgcgccgtgt tacggccgag gcgcggcggc cgttcgagct cgcatccggg ccgctgatcc
     4021 gcgggacgct gctgcggctg gccgcggagg accacgcgct gctggtgtcc atgcaccaca
     4081 tcgtcaccga cggctggtcc atgggcatct tcctgcgcga gctgatcact ctctataccg
     4141 ctctctctcg gggccggtcc ggcgaactgc ccgagctgcc ggtgcagtac gcggacttcg
     4201 tggtttggca gcgctcctgg ctcgcaggcg aggtcctgga ggcgcagctc gcgtattggc
     4261 ggcagcgcct gaccggcgcc ccggccctcc tcgagctgcc caccgaccgc ccacggccgc
     4321 tggtggagac ccatcacgga gccgcgctgc ctctggcgct cggtccgtcc ctgtcgcgcg
     4381 cgctggtggc gttggcccgg cgggaagggg cgacgccgtt catggtcctg ctggcgggat
     4441 tcgcggcgct gctgggccgc tactccggcc aggacgacgt ggtcgtgggc tcccccatcg
     4501 cgggccggaa ccggcgcgag atcgaggggc tgatcgggtt cttcgtcaac acgctggctc
     4561 tgcgcgtgga gctgtcacag gggctgggct tcctggatct cctgcggcag gtgcggcaga
     4621 cggcgctgga ggcctacgct caccaggaca taccgttcga gcggctggtg gaggagctgg
     4681 tgaccgatcg cgggctgggg cactcgccgc tcttccaggc gactttgacc gtgcagaaca
     4741 cacctccggt cgccgtggag ggggccgggc cggagttgga gatgcgcctg ctgggcgtgg
     4801 agtcggcgtc ggccaagttc gagctgagcc tggatttcgc cctcgccgga gaggagctgg
     4861 cgggaggtct ggagtacaac accgacctgt tcgatgcctc tacggcgcgg cggctggcga
     4921 gccacttcgc caggctcctg gagggcgctc tggcggcgcc ggagagcctc ttgccggaga
     4981 tcctcctgct ggcgccggcg gagcggcagc agctcctccg cgagtggaac gacacggagg
     5041 cggcctaccc ggcggaggag ctgtgcctgc acgagctggt ctggcggcag gcagaacggg
     5101 cgccggacgc ggtggccgtc accctggcgg aggagcacct ctcctacgcc gagctgcgcc
     5161 ggcgatcggg ctggctcgcc catcgcctgc ggcgcctggg tgtcggcccc gaggtgccgg
     5221 tggccatcgc cgtcgagcgc tcgccggaga tggtggttgg cctgctgggc atcctccagg
     5281 cgggtggcgc ctatgtgcct ctcgatccgg actatccggc cgagcgcctg tcctacatgg
     5341 tcgaggattc ggcggccccg ctgctcctca cccagcggca tctcgtggaa cggctgccgg
     5401 agagcggcgc ccgcgtggtg ttgctggacg aggcggcgcg gccggagccg ggtctgccgc
     5461 cggaagctcc cagcggtgtc ctcgccgata acctgctgta cgtgatctac acctcgggct
     5521 cgacggggcg gcccaagggg gtcatggtgc ggcaccgcgg ggtggtgaac cgcctcctgt
     5581 gggccgtcca ggactatccc atcacgtctg cggaccgggt cctgcagaag gcttcgttca
     5641 gctttgactt ctcggtggag gagtgcttca gcccgttgtt ggcgggagcc cgcctggtcc
     5701 tggcccgtcc tggcggccaa cgcgacagcg cctacctcct cgaggccgtg gtggaggagg
     5761 agatcaccct cctgcacttc gtgcccgcgg tgcttcaggc cttcctggcc gaggaggggc
     5821 tggaggcctg ccacagcctg cgtttcgtct tctcgggggc cgacaccctg acgccggagc
     5881 tgcgggagcg cttcgaagcc cggataccgc cgggggtcct gctgcgcaac cagtacggcc
     5941 cgacggagat ctccatcgac gtgacggagt gggtctgccg tcccggcgag ggacggttcg
     6001 tggctcccat cggccgtccc atcggcaacg gtctggcgta tgtggtggac tcccgtctgg
     6061 agccggtgcc catgggcatc gcgggcgagc tcctgctcgg cggcgcgggc ctcgcccggg
     6121 gctaccaggg gcgtcctgat ttgacggcgg atcgcttcgt gccgtcgccc tgggggaggg
     6181 aggcgggcgc gcggctctac cgcacggggg atcgggtccg tctgttgccg gacggcaacc
     6241 tggagttcct ggggcgcgtc gaccaccaga tcaagatccg cggctatcgc atcgagccgg
     6301 gggagatcga agccgtgttg gcgacgcatc cggcggtcca cgaggccgtc gtggtggcgc
     6361 gggaggatcg gaagcgcggc cgctctttgc tggcttgcgt ggtgccggcg gcgggtgccg
     6421 tggtggtgcc ggaggagctg cgctcgttca tgcgtgagca gctccccgac tacatggtgc
     6481 catcggcgtt cgcggtcctc gaggtgctgc cgttgggtcc cagcggcaag gtggaccgca
     6541 aggctctggc acagctcgcc acgatcgcgg aggagcatgc ggaggatcgc ctcccgggtg
     6601 gcggtcaggc cgcgccacgc acgccttcgg aagagatcct ggcggccatt ttcgcggagg
     6661 tcctgcggcg cgagcgggtg ggggtccacg acggcttctt cgactttggg gggcactcgc
     6721 tgctggcgac tcaggtggtg tcgcgggcgc gccaggcctt cggagtggag ctgccgattc
     6781 gtgatctgtt cgaggcgccg acggtggccg gactcgcgga gcggatcgag cggctacggc
     6841 gggcaggtgc cggagtggaa gctccgccca tcctgccggt gccccggcaa ggcggcctgc
     6901 cgctgtcctt tgcccagcag cggctctggt tcctccaccg gctggcgccg gacagcgcgg
     6961 tctacaacgt
//
LOCUS       DASPJI010000584         3325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_384924, whole genome shotgun sequence.
ACCESSION   DASPJI010000584 DASPJI010000000
VERSION     DASPJI010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384924"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            380..610
                     /locus_tag="VEL74_16335"
     CDS             380..610
                     /locus_tag="VEL74_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64145.1"
                     /translation="MKRIRRFAIAFAVVAATGVAGFTSTAPAGACCRPCAGYCGTPGV
                     PLSAACCSGVSTPGNACGFTNCCSYLGICAEK"
     gene            complement(619..1959)
                     /locus_tag="VEL74_16340"
     CDS             complement(619..1959)
                     /locus_tag="VEL74_16340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG64146.1"
                     /translation="MAGDVDEEAPLTPGPFHSLRVAGRVLRAEGARAVRDRALDRLAE
                     ERRRRSFTGSALPALSIPVLNLATAAPAARLGGVQGQLLHRVEAESALRPLALLYPEG
                     GSHRLEVLAGGSRHALQLGGGFESAVETAAGSTGARALHVEGLAGLPMGSLLALRRQG
                     LRLILSLHDFSPFCPRPHLLEHPVLRFCHYSRDMDRCGFCLRQDWPVDGDWQAERREL
                     AAELLAEADSLVFPSEFLRRTYRGLFPALDPGRQHVIEPAAAWEPVPPRPAPAVPVRH
                     VALVGGVQVHKGALVIEELVRRLADRPDLRWSAYGGGDATILERLRRLPRMNVRGYYR
                     SGSLPSLLRRGRVDLALLLSIVPETYSLVLTECQAAGVPVLAFDLGAPGDRLRAEGGG
                     LLAPPESGAEGVAALLRDFLENRAAIPPLPTPPESNARRVAETWLRLYRDLGLT"
     gene            complement(1926..>3325)
                     /locus_tag="VEL74_16345"
     CDS             complement(1926..>3325)
                     /locus_tag="VEL74_16345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG64147.1"
                     /translation="IRPLRQRVLAEIRPPKRPQPPGRPERVLHVVHGWPPWNHAGTEM
                     YAAWLARRQAEVRDVAVYSRIADPERAHGDAVETLDRGVRVRSVVNNFTQRDPLSRNG
                     LRDRTIEADFARFLDQFNPRVVHVHHLAGHSAGLLRVAARRKVPVVYQVQDWWAPCAR
                     VNLFDAWRRLCSGPGIGKCSACRPLTGVPPAPVLNRLLYSHRLSQFRKLLGIPEAFVM
                     GSRFILDSYLELGFLDKDSLVFVIPYGVELGPIPDARPARTPGAPLRFGLIGSILPHK
                     GIHVAVEAFAGIDPARATLTVWGDPSIDPDYTRELEGLRGPGTEVRERFEDDRKAEVF
                     GSLDALIVPSLGLESFGLVAREAMHHSVPVLATRRGALSEMFLGGDFGALFNPEEPAE
                     LRGWIERLAADPGILDRWRERLPTVKGMEQHTEEMEQVYEKVLADKILLDRTLTGKFS
                     RKKGKWPATWTKKRR"
BASE COUNT          485 a         1169 c         1236 g          435 t
ORIGIN      
        1 tccgggggta tggccgccgt acccaccgaa acgttcgtcg taccccggca atcgttcgcc
       61 ctacccgggg tacggtcttc gtctccaggg ggatgacgtc catacccagg ggatggaggg
      121 tctacccgcg gggatgacct ccgtacccgg ggtacggaag ccgtacgcgt ggggacgccc
      181 tccctacccc gggtagggtc gtcgcgccct cggggatgga cttcgtttct gcggggatgg
      241 tggccggcgc ggcgcgcgcc tcctgaaaga aaaccccctc ctcggacctc ccccgctccc
      301 tcccccagga gagagcggag ctcgtgatgg agtgcgcacg tgtagaatag gcttgaacgt
      361 tgagctggaa ggagacccca tgaagagaat ccgccgcttc gccatcgcct tcgccgtcgt
      421 cgccgccacc ggagtggccg gcttcaccag caccgccccg gccggcgcct gctgccgtcc
      481 gtgcgcgggc tattgcggca ccccgggcgt gccgctctcg gctgcgtgct gcagcggggt
      541 ctcgacaccc ggcaacgcct gcggcttcac caactgctgc agctatctgg ggatctgcgc
      601 agagaagtag ggacggcttc aggtaagacc cagatcgcgg tacagccgca gccaggtctc
      661 ggcgactctc cgggcgttgc tctccggcgg ggtcgggagc ggtgggatcg cggcccggtt
      721 ctccaggaag tcgcgcagca gggcggcgac tccttcggct ccggactccg gcggcgccag
      781 caggccccct ccttcggcgc ggaggcggtc gcccggggcc cccaggtcga aggcgagcac
      841 cggaactccg gcggcctggc actcggtgag gacgaggctg taggtctcgg ggacgatgga
      901 gaggaggagc gcgaggtcca cccggccgcg gcggagcagc gagggcaagg agccgcttcg
      961 gtagtagccc cggacgttca tccgcggcag ccggcggagg cgctccagga tcgtggcgtc
     1021 ccctccgccg taggcgctcc agcggaggtc cggccggtcc gccagacgcc ggaccagctc
     1081 ctcgatgacc agggcgccct tgtggacctg gacgcctccc accagcgcca cgtgccggac
     1141 ggggacggcg ggggcgggac gcggggggac cggctcccag gcggcggccg gctcgatgac
     1201 gtgctggcgg ccggggtcca gggcggggaa gaggccgcgg taggtccgcc ggaggaactc
     1261 cgaggggaag accagcgagt cggcctcagc gagcagctcc gccgccagct cccgccgctc
     1321 cgcctgccag tcgccgtcca ccggccagtc ctggcgcaga cagaagccgc agcggtccat
     1381 gtcccgcgag taatgacaga agcggaggac gggatgctcc aggagatggg gccgcgggca
     1441 gaaggggctg aagtcgtgga gcgagaggat cagccgcagg ccctggcggc ggagggccag
     1501 gagcgagccc atggggaggc ccgccagacc ctccacgtgg agcgcccgcg ccccggtgct
     1561 gccggcggcg gtctcgacgg cgctctcgaa gccgccgccg agctggagcg cgtgacgcga
     1621 tccccccgcc agcacctcca ggcgatggct ccccccctcg ggatagagga gggccagggg
     1681 acgcagggct gattcggctt cgacgcggtg gaggagctgg ccctggacgc ctcccaggcg
     1741 ggcggccggc gcggcggtgg cgaggttcag gacggggatg gagagggcgg ggagcgcgga
     1801 cccggtgaag gagcgccggc gccgctcctc ggccagccgg tccagcgccc ggtcgcggac
     1861 cgcccgtgct ccttcggccc gcagcacccg gccggccacc cggaggctgt ggaagggccc
     1921 cggggtcaac ggcgcttctt cgtccacgtc gccggccact tccccttctt gcgcgaaaac
     1981 ttgccggtga gggtccggtc cagcaggatc ttgtccgcca gcaccttctc gtagacctgc
     2041 tccatctcct cggtgtgctg ctccatcccc ttgaccgtgg gcaggcgctc ccgccagcgg
     2101 tccaggatgc ccggatcggc ggcgaggcgc tcgatccagc cccggagctc ggccggctcc
     2161 tcggggttga acagggcgcc gaagtccccg ccgaggaaca tctcggacag cgccccgcgg
     2221 cgggtggcca gcaccggcac gctgtggtgc atcgcctcgc gcgccaccag tccgaacgac
     2281 tcgaggccca gggacggcac gatcagcgcg tccagcgagc cgaacacctc ggccttgcgg
     2341 tcgtcctcga agcgctcgcg gacctccgtg ccagggccgc gcagaccctc gagctcccgg
     2401 gtgtagtccg gatcgatgga cggatccccc cagacggtga gcgtcgcccg cgccggatcg
     2461 atgccggcga aggcctccac cgcgacatgg atccccttgt gggggaggat cgagccgatc
     2521 agcccgaagc gcaatggcgc gcccggcgtc cgcgcgggcc tcgcatccgg aatgggtccc
     2581 aactccacgc cataggggat gacgaagacc agggagtcct tgtccaggaa gcccagctcc
     2641 aggtagctgt ccaggatgaa ccgggagccc atgacgaagg cttcggggat acccagcagc
     2701 ttgcggaact ggctcagacg gtgcgagtag agcagccggt tcagcaccgg cgccggcggc
     2761 acgccggtca gcggacggca ggcggagcat ttgccgatcc cggggccgct gcacaggcgc
     2821 cgccaggcgt cgaacaggtt caccctggcg cagggcgccc accagtcctg gacctggtag
     2881 accaccggca ccttgcgccg ggccgccacc cgcaggaggc cggcgctgtg cccggccagg
     2941 tggtggacgt ggaccacgcg cgggttgaac tggtccagga aacgggcgaa gtcggcctcg
     3001 atcgtccggt cgcgcaggcc gttcctggac agtgggtcgc gttgcgtgaa gttgttgacc
     3061 accgagcgaa cgcggacgcc gcggtccagg gtctcgacgg cgtcgccatg ggcccgctcc
     3121 gggtcggcga tgcggctgta gaccgcgacg tcccgcacct cggcctgccg gcgggcgagc
     3181 caggcggcgt acatctcggt gccggcgtgg ttccaggggg gccagccgtg gaccacgtgc
     3241 agcacccgct ccggacgtcc ggggggctgc gggcgcttcg gcggccggat ctcggccagc
     3301 acccgctgcc gcaaggggcg gatcg
//
LOCUS       DASPJI010000585         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_207858, whole genome shotgun sequence.
ACCESSION   DASPJI010000585 DASPJI010000000
VERSION     DASPJI010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207858"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VEL74_16350"
     CDS             <1..405
                     /locus_tag="VEL74_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64148.1"
                     /translation="GSGVGFLTAPVALTCGASTATSLCLHGRYLVTSFFRTNPTPGTP
                     TDGQGRVVPVANPGSGIFWFFAADNWELMVKVLNGCGLNNRWWVFSAATTNVFYRLEV
                     DDVRTGEQKIYFNYPGPPAPAVTDTDALATCP"
     gene            complement(464..1813)
                     /locus_tag="VEL74_16355"
     CDS             complement(464..1813)
                     /locus_tag="VEL74_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64149.1"
                     /translation="MNRIVALLLIAGWVAAAGAAAQPVGCNLAPNPAFERGVSSAIES
                     WGLARLSGTATLALSDYPEPVQSGARAARVDVQESGDISLRTAEPGSIQVLPGTAYRL
                     SARLRSEPGKTAGIRVVEWSHRRGTVADRFLAFNAGTGDWETVEGTFTTTEATAFLSI
                     RLMHHVHAGTFYWDDVLLSVERPGERCMDIRHYLMQTTPGFKMCLDNRPGFCHDGVKL
                     PSGEFLREQPAARDYLSHTLRSGAMVGVIKNFDAEYGAWRCFTDPGDPCDAVLAAEGE
                     NPRFPPMPLGVSIPLPVLAAMGSDRVTPGAALLHDWQPFDVRNFKTDWTSGPKIGTIR
                     HRTWAYLLASHIFTGNLGTHENVLVIETESGGDVVRAAGVGGGTRLERYIFVRGIGLA
                     YAEGRESPDCGASPGAATCNGAYAKIHPAAARFTLKTPGDYPFSTDHPPSYNVVDWW"
     gene            complement(1840..>2465)
                     /locus_tag="VEL74_16360"
     CDS             complement(1840..>2465)
                     /locus_tag="VEL74_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64150.1"
                     /translation="AQVGDQPVRPVERGGMLSVPLGFEGGTSVIEVVSVQEKAVPPGR
                     SRLALDLPQVRVPVLDHRWRLLLPEGSRYRFRAGELRPARDEYLEGGVEETVVTGGVV
                     GGVVGGVPGGVVTETEMLPPPPPPPPAPVEVPQSISDAPLRVEERQRRDAPREQLQKE
                     LDEIRQGLVGGVKPLPVSIPEDGKLLLLTGVLPPERVAVELEVKGKR"
BASE COUNT          375 a          903 c          828 g          359 t
ORIGIN      
        1 ggctcgggcg tgggcttcct caccgcgccg gtggccctca cctgcggcgc cagcacggcg
       61 acgtcgctct gcctccacgg ccgctacctg gtgacctcct tcttccggac caacccgacc
      121 cccggcacgc ccaccgacgg ccagggccgc gtcgtccccg tggccaaccc cggctcaggc
      181 atcttctggt tcttcgctgc cgacaactgg gagttgatgg tcaaggtgct caacggctgc
      241 ggcctcaaca accgctggtg ggtcttctcc gccgccacca ccaacgtctt ctaccggttg
      301 gaggtggacg acgtgaggac cggcgagcag aagatctact tcaactaccc cggcccgccg
      361 gcgcccgccg taaccgacac cgacgctctc gccacctgcc cgtaggctcc agagccgggc
      421 cgcctcgtcc gaggcggccc cggctgtccg tcagccgctc ccctcaccac cagtccacga
      481 cgttgtagga cggcgggtga tcggtggaga atggatagtc gccgggggtc ttcaaggtga
      541 aacgcgccgc ggcggggtgg atcttggcgt aggcaccgtt gcaggtggcg gcgccggggg
      601 aggcgccgca gtccggggat tcgcggccct cggcgtaggc caggccgatg ccacggacga
      661 agatgtagcg ctccagccgg gtcccgccgc ccactcccgc ggcgcggacc acgtcgccgc
      721 cgctctcggt ctcgatcacc agcacgttct cgtgggtacc caggttgccg gtgaagatgt
      781 ggctcgccag cagataggcc cacgtccggt ggcggatagt gccgatcttc gggcccgagg
      841 tccagtccgt cttgaagttg cggacgtcga agggctgcca gtcgtggagc agcgcagccc
      901 ccggggtgac ccggtccgac cccatggccg cgagcaccgg aagggggatc gagacgccga
      961 gaggcatggg cgggaaccgg gggttctccc cctccgccgc gaggaccgcg tcgcacggat
     1021 cgccgggatc ggtgaagcac cgccaggcgc cgtactccgc gtcgaagttc ttgatcactc
     1081 cgaccatggc gccggaacgc agggtgtggg agaggtagtc gcgcgccgcc ggctgctcgc
     1141 gcaggaactc gccgctgggc agcttgacgc cgtcgtgaca gaagcccggc cggttgtcga
     1201 ggcacatctt gaagccgggg gtggtctgca tcaggtagtg ccggatgtcc atgcagcgct
     1261 ctcccgggcg ctccaccgac agcaggacgt cgtcccagta gaacgtgccg gcgtggacgt
     1321 ggtgcatcaa ccggatggag aggaaggccg tggcctccgt ggtggtgaag gttccttcca
     1381 ccgtctccca gtcgcccgtg ccggcgttga aggcgaggaa ccggtccgcg accgtaccac
     1441 gccggtggct ccattcgacg acccggatgc cggcggtctt gcccggctcg gaccggagcc
     1501 gggcggacag ccggtaggcc gtgcccggca ggacctggat gcttccaggc tccgccgtac
     1561 ggagagagat gtcgccgctc tcctggacgt ccaccctggc ggcgcgcgcc cccgattgca
     1621 ccggctccgg gtagtcggag agggccaggg tggccgtgcc cgacaaacgc gcgaggcccc
     1681 atgactcgat ggccgacgag accccgcgct cgaacgccgg attgggagcg aggttgcacc
     1741 ccaccggctg ggccgcggct cctgccgccg ccacccagcc cgcgatcagc aggagcgcca
     1801 cgatccggtt cattacagct ctagaaccgg aagcaccccc tacctcttcc ccttgacctc
     1861 cagctccacc gccacccgtt ccggcggcag gacacccgtg aggaggagga gcttgccgtc
     1921 ctcggggatg ctcaccggca gcggcttcac ccctccgacg agaccctgcc ggatctcgtc
     1981 cagctctttc tggagctgct cccggggcgc atcccgccgt tgtcgctcct ccacccgcaa
     2041 gggcgcatcc gatatagact ggggcacctc caccggcgca ggaggaggtg gtggcggtgg
     2101 cggcagcatc tcggtctccg tgaccacacc tccgggtacg cctcccacta caccgccgac
     2161 aacaccaccc gtcacgacgg tctcttcgac gcccccttcg aggtactcgt cccgggcggg
     2221 acggagctcg ccggcgcgaa agcggtagcg cgagccttcc ggcagcagca ggcgccagcg
     2281 gtgatcgagc accggcaccc tgacctgcgg gaggtccagg gcgagacgcg agcggcctgg
     2341 cggcacggcc ttctcctgca cggagacgac ctcgatcacc gaggtcccac cctcgaaacc
     2401 gagagggacc gacagcatgc caccccgctc caccggccgc accggctggt cgcccacctg
     2461 cgccg
//
LOCUS       DASPJI010000586        16562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_385084, whole genome shotgun sequence.
ACCESSION   DASPJI010000586 DASPJI010000000
VERSION     DASPJI010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16562)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16562
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385084"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VEL74_16365"
     CDS             complement(<1..564)
                     /locus_tag="VEL74_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004447869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HYG64151.1"
                     /translation="MQVFHRSTNVIAKVSIFGAIFFLGLLGWIVSTLLRSSYATGQSV
                     VIKQPVPFSHDHHVAGLGIDCRYCHTSVEKAGFAGIPPTETCMNCHKQIWTNADLLEP
                     VRASYRENVPIQWNRVHDLPDFVYFNHSVHVAKGIGCVTCHGQVDRMPLMYQESSLLM
                     EWCLDCHRDPGQHVRPRDQVFNMNWQPP"
     gene            complement(856..1656)
                     /locus_tag="VEL74_16370"
     CDS             complement(856..1656)
                     /locus_tag="VEL74_16370"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64152.1"
                     /translation="MDRWLPPNAAAHGAQIDAALSWVHLLMAAVFAIGLAFFLTALWR
                     FRRSRHPAASHAGLTGRFPAWLEAAVVVAEGALLVGVSMPLWAARVERIPPERDAVVV
                     RVVSEQFAWNVHYPGPDGRFGRTALHLIDLQRNPLGLDRADPAAADDVTTVNQLYLPV
                     DRPVIVHLSSKDVIHSFNVPQFRIKQDAVPGLSIPVWWVPTVTTEEMRRRLGQPGFVY
                     EIACAQLCGAGHYRMRGFVTVLEQDEFKTWLEGQVADPPPTSLLTPAD"
     gene            complement(1656..1913)
                     /locus_tag="VEL74_16375"
     CDS             complement(1656..1913)
                     /locus_tag="VEL74_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64153.1"
                     /translation="MATPSARRLAVAVLALGLLAALPAAACTSCYGAADAGAPLISGA
                     RLGVFLLLGITLAVLAGFVRFFLYLRKRARQAGHDGVEQAA"
     gene            complement(1929..2525)
                     /locus_tag="VEL74_16380"
     CDS             complement(1929..2525)
                     /locus_tag="VEL74_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HYG64154.1"
                     /translation="MASEIPYTSAERPDTGLTNGRIAVWLFLASEVMLFGALFASYVM
                     LRAGAPSWPRPAALLDVPLATVNTLVLIASGVTMALARRAIRGGDLRGFRRFMGGTVL
                     AGGLFLILESVEWAARIERGLLPSTNTFLALVFTLTGLHALHVLGGLAVNGYLWGPGA
                     RLQGSDTRHFALRVENAALYWFFVDLVWIVLFPVLYIL"
     gene            complement(2529..4325)
                     /locus_tag="VEL74_16385"
     CDS             complement(2529..4325)
                     /locus_tag="VEL74_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HYG64155.1"
                     /translation="MSKQLEPLATVAPARPETATDEAASQARPGFWRRAVFSTDHKVI
                     GIQYAVTGLLFLFFGFCLVMIMRWQLAYPGRPLPWLGRLLGEARMPGGFMLPETYNEL
                     GALHGTIMVFLGVVPLAVGGFGNFVLPLQIGAPNMAFPRMNRASYQAYLLAGVTMLAS
                     FFVPGGGPQNGWTSYPPLSVIAGHGQTMWLLGLLFLGASTLLSAVNFIVTTVQLRAPG
                     LSFFRLPFFVWAQLVTAFLLLLAYPPLEAAAVLQLMDRLAGTSFFLPSGLSVTGQPLD
                     VSGGGNPLLWQHLFWFLAHPEVYVLILPAFGILAEVIANNIRRPLSGYRPMVYGVWFL
                     GFMSFLVWAHHMFLTGMGTTMAVFFQATTMIISIPSVVLLTSLLLSLWGGSIRFTPPM
                     LFALAFLPMFALGGLAGLPLGLSATDIPLHDTYYVIGHFHYIVAPGTLFALFAGIYHW
                     FPKATGRMMSERLARLHFWPSLLFMNGIFLPMFIQGLGGVSRRLYDGGASYLFARDVI
                     QWNVFMSVSAWCLALAQIPFVFNFFYSLRRGRKAGDNPWEATTLEWSAPSPPPPGNFT
                     TAPVAHRGPYEYSVPGDPGDFTPQFQPAPKAG"
     gene            complement(5274..5501)
                     /locus_tag="VEL74_16390"
     CDS             complement(5274..5501)
                     /locus_tag="VEL74_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64156.1"
                     /translation="MGSDNGHRPPVGFLQLFDKKAVDALNVVIHLLQAPVCLAYVLEA
                     AGGVTLERCGAILDERVAEVRAENTAGAAAG"
     gene            5712..6545
                     /locus_tag="VEL74_16395"
     CDS             5712..6545
                     /locus_tag="VEL74_16395"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYG64157.1"
                     /translation="MWAALTAAAAIAEILSLLLAWGAYRRRRNLKKELAELRLVPEEE
                     RARLLERKLDRFGVAAAGLSKKDSFLIIQQTIERQAERFRRALWAGVSACLIFAALTA
                     YAWTLDQSPDLYRLRVTVLAPDGRPSNNAFVSTSVGGEILKAHQGWEILLPRNRRGQA
                     FTVFASYPEAFLQGRQEVALGDDFEPPAIEIQLSRREVEVRGTVIDPQGHGVAGATVS
                     VVGFEQESVGTDTSGNFRLQAHAADRQTVRLRVVKDGRSSEFEARAGDLPAQQLVWEE
                     P"
     gene            6542..9346
                     /locus_tag="VEL74_16400"
     CDS             6542..9346
                     /locus_tag="VEL74_16400"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HYG64158.1"
                     /translation="MKKIIPFLLWAVMWSVTGPTAAEDRILRVQVVDVAGVPQARLLI
                     GLAGEGTFQSTDQQGNARLPLPAQARVGDPVTLRLSNKALVFISPSEGRTHIPSFDEK
                     PGNFVTALVGKLGELTLLKSGDVLRSFLNQVLGEVERLQERSTEATATQLRTVLDERA
                     GGFGLNAEDFDRAIRAWLEAPVGLDDKRLAARYLELYPEPVSQNSYGSVVVGEGASMK
                     DSLIVTNPQNSPITIQNRRHDVDSALSVIARAVEARPTGDIGQIAAAEFLAGQEGPGL
                     KGMDLAGLSFTGATLETAKLSEAILDGADFSHANLQKADLSSAKLGFSTLQEATLAEA
                     LLEGTRFYFVSATKAIFDNARAAGSNWTGADVRGASFRGADLRGASFAFTDLTGADFT
                     GADLTETLFISAILTDVRFDGATVRNTDATAAVGDVSIFTPVQQQELCATGLGQGSGQ
                     VELVRVVPSTRFSSGSDYDRLTYGNLPALEALHLLDPCTLRIHRPAGFETVWSAGNNE
                     YVKTAYAFHYPSDLLDKGSRSGKFAARLKEQLKRLDEAMARGPFIAVRGKTHAALARA
                     LKANLGKTAPLAPLDLFDEETIFLLTLRFLPKEADKIDWHGRAQNRMRDEQYRKAHPD
                     DGPSIWPIFFPEGFSSNQLGAEHVELYRQWTQGRLKRLPLEFTLTTRFEGGARSRSRR
                     TPQPETAGAPVRAALLASFGEPLPDGLLGKVGGGAADGFAFDVHGEDYALVFAQSRAA
                     YVLDIPGEIAEELATQNPQGEVRIRFQGAEIVELESRRYPEQQENDRMTVFRVEPISI
                     RLVGRQGLTFPSSPAEAEKLRIIPPKQAEKRIEFRRRLCTYYTVPASALANLRDKEAP
                     DLPSIQSMLSDDPSAESLKTQEYLLRQLNDNPGYADILADYFIQTIASGESKEDEYLG
                     PIYKRKLGEFEPLKPPASCR"
     gene            complement(9686..10615)
                     /locus_tag="VEL74_16405"
     CDS             complement(9686..10615)
                     /locus_tag="VEL74_16405"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYG64159.1"
                     /translation="MPRKIRHVPEGGSLVEVTCRTVQSRLLLAPRAYVREIVLGVLAR
                     AKRLYPVQLICFAFTSNHYHLLIHVEDAGRMAEFMGYLNGNLAKEVGRLADWKEKFWG
                     RRYQHIVVSDEEAAQVARLKYILSHGAKEGLVADPREWPGAHAVNALLTGDPLEGVWY
                     DRTQEYRYRLRGKEPAPRQFATVETLTLDPLPCWKHLPAEEYRQRMADLVAEVVEEAR
                     AEREAKGLEPMGADAVRRQQPHTRPNRTKKSPAPLFHTASKKAYLNLREAYSRFLAAF
                     RDAAEKLKAGDRTVEFPEGSFPPALPFVRPAFG"
     gene            complement(10682..11056)
                     /locus_tag="VEL74_16410"
     CDS             complement(10682..11056)
                     /locus_tag="VEL74_16410"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG64160.1"
                     /translation="MVIDTSVLLAIFFEEPQAEWAVARLEEHAPDLRMSTVNLTEALI
                     RIRDRQPRLYERIEAELLSSGIRFIPPDVRQAQIAAEARLRLPLNLGDCFAYALAAVE
                     DCPILAIDGDFRGIDRPVLMPS"
     gene            complement(11061..11297)
                     /locus_tag="VEL74_16415"
     CDS             complement(11061..11297)
                     /locus_tag="VEL74_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64161.1"
                     /translation="MPQLNLHLTEEFEEALDELMRLRQIRTKSEAVRWAVLSAAERER
                     RRRAPTEFSKWLGLGHREPENPSPRFRSDDDLWG"
     gene            complement(11341..11931)
                     /locus_tag="VEL74_16420"
     CDS             complement(11341..11931)
                     /locus_tag="VEL74_16420"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HYG64162.1"
                     /translation="MSSQGADYDWGDRLPTLTGPRVELRWLGEEDVPALFEIFSDPEV
                     MRYWDSPPLGDLAAAAGLLREIHDHFRARTLFQWGIAPRGGGAVLGTCTLFRWEARHR
                     RAEIGFALGRAHQGKGFAAEAVALLLSFAFQSLGIHRLEADADPRNEPSLRLLERLGF
                     RREGYLRERYHTNGEVQDSVILGLLRPEWEVASSPT"
     gene            complement(11953..12507)
                     /locus_tag="VEL74_16425"
     CDS             complement(11953..12507)
                     /locus_tag="VEL74_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HYG64163.1"
                     /translation="MTPIPSAEDIVKATKTWLERAVIGLDLCPFAKSVHLKDQIRYTV
                     SEATTPEDLLEHLVIELRAIAAADPEEVETTLLIHPGVLVDFLDYNDFLDVADTAVAE
                     LGLEGEIQVASFHPRYQFAGTAPEDIENYTNRSPYPILHLLREASVERAVASFPETER
                     IYEKNIETLRRLGHEGWRRLWVAD"
     gene            12582..13079
                     /locus_tag="VEL74_16430"
     CDS             12582..13079
                     /locus_tag="VEL74_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HYG64164.1"
                     /translation="MARYKMRQRLIAIGEDFDIQDEQGRPIYHVDGKVLRIRETFVIQ
                     DLSGNEVATIREKKIALRDTMNILRGGEVVATIRKAWITPLRDKFAIDVQNGPDMTAQ
                     GNILDHEYEIRQGGETIARVSKHWFTIRDTYGIEIAPGHDDGLILAMAVAIDEMAHDP
                     DEGKG"
     gene            13098..14438
                     /locus_tag="VEL74_16435"
     CDS             13098..14438
                     /locus_tag="VEL74_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYG64165.1"
                     /translation="MSFDLWFLVLGGLLVVIALIASVVRHLPLTTTVLYLLVGLGLGA
                     FGIGAIRLDPLRHAALLERVTELAVIVSLFTAGLKIRLPVHDPLWRLPFRLASLSMVV
                     TVGLITLVGTWGLGLPLGAAVLLGAILAPTDPVLASEVQLAAAGDRNRLRFGLTGEAG
                     LNDGTAFPFVMLGLGLLGAHEIGEWGWRWLAVDVVWAVAAGLGIGLVLGTLVGRLVLF
                     LRKKHKEAFGLDDFLALGLIGLSYGAALLLHSYGFLAVFAAGLALRRIERKASPDEED
                     PGAAQENLRAMAAAGKEEEIATDPEKAPAYMAQAVLGFNEQVERIFEIGVVLLVGAML
                     SWRFLPGSALWFVPVLFLVIRPLAVLTGLAGARLPALDRGLMSWFGIRGIGSIYYLMY
                     AIEHGLDPALAEPLTAVTLTTVAASILIHGISVTPLMKHYEKRLRKRGEAPAAT"
     gene            complement(14450..15505)
                     /locus_tag="VEL74_16440"
     CDS             complement(14450..15505)
                     /locus_tag="VEL74_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64166.1"
                     /translation="MTAEPAPDLHRRPSPSPSEIASWILMGAGLLLVVHLRLLAALIG
                     GLAVYELVHILVRRLRFLGSGAPDGRTDARKYVAVTLLAVVIVAGLTFSIFGLIAFFR
                     SGPGNLPALLAKMADIVEGSREHLPLWLAREIPANAEELKNEAVAWLREHAGELQMFG
                     AEVGRALVHALIGMIIGAMVSLNETRRVQRHAPLASALTEQAGRLGEAFRRIVFAQVR
                     ISALNTLLTATYLVVVLPVLGIRLPFTKTLIAITFLAGLLPVIGNLISNTVIVVVSFS
                     YSLGVAAGSLAFLVVIHKLEYFFNARIVGGEIQARAWELLLVMLAMEAAFGIPGLIAA
                     PIYYAYLKSELSARGLV"
     gene            15573..>16562
                     /locus_tag="VEL74_16445"
     CDS             15573..>16562
                     /locus_tag="VEL74_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:15819..15821,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HYG64167.1"
                     /translation="MPKRNVRLTTPLVRENGALRPATWDEALDRAAQGFRSAVEAKGP
                     QTFGLFSCSKTTNEMNYLAQKFARAVVGSNNIDSCNRTUHAPSVVGLATVFGMGGGTT
                     SYREVEETDLVILWGSNARETHPIFFHHVLKAIHNGARLYAVDPRRTSSADWATGGHL
                     PLHVGTDVALANAVAREILAAGLENRSFIDNATSGFEEFRTHVEPYTLEYAEAVTGVP
                     AATIRDLAHAYARADRAVLCWTLGITEHHNAVDNVRALINLALLTGHVGRWGSGLNPL
                     RGQNNVQGGGDMGALPDRLPGFQHVENDELRANFERVWGVPIPPKRGWHLSGMF"
BASE COUNT         2935 a         5516 c         5517 g         2594 t
ORIGIN      
        1 cggcggctgc cagttcatgt tgaacacctg atcgcgcggc cgcacgtgct gccccgggtc
       61 gcggtggcag tccaggcacc actccatcag gagggaggac tcctggtaca tcagcggcat
      121 gcggtcgacc tggccatggc aggtgacgca gccgatgccc ttggcgacgt ggacgctgtg
      181 gttgaagtag acgaagtccg gcaggtcgtg gacccggttc cactggatgg gcacgttctc
      241 gcggtagctg gcgcgcaccg gctccagcag atcggcgttg gtccagatct gcttgtggca
      301 gttcatgcag gtctcggtcg gcgggatgcc cgcgaagccg gccttctcga ccgaggtgtg
      361 gcaataccgg cagtcgatgc ccaggccggc cacgtggtgg tcgtggctga acggcaccgg
      421 ctgcttgatc accacgctct ggccggtggc gtacgacgac cggagcaggg tcgagacgat
      481 ccagcctaac aggccaagga agaagatcgc gccgaagatg ctgaccttgg cgatgacgtt
      541 ggtgctgcga tggaagactt gcatcggacg gagaccctct cctcctagcc gcgcaaaggc
      601 cggcgaattc ggaccgttgt caggggctca ggggactttg tgaaagtcgt cacaagcacg
      661 catcctaccc aacctgccgg tctcgtgcaa tacgggtgcc cctccgctga gcggcacgcc
      721 cccactccct ggcgtttccc ccctcccccc tcggacggac gccgccagcg ccaccctggg
      781 gcggccggtg atggacgggt tcgacttcga aggggacctc ccttttgggg aaaacgacgg
      841 tgcagatctt acccttcaat ccgccggagt caacagggaa gttgggggcg ggtccgccac
      901 ctgtccttcc agccatgtct tgaactcgtc ctgctccagg acggtcacga agccgcgcat
      961 ccggtaatgt ccggcgccgc agagctgggc gcaggcgatc tcgtagacga agccgggctg
     1021 gccgaggcgc cgccgcatct cctcggtggt gacggtgggc acccaccaga ccgggatgga
     1081 gagccccggc accgcgtcct gcttgatccg gaactgcggg acgttgaagc tgtggatcac
     1141 gtccttgctg ctcaggtgga cgatcaccgg ccggtccacc ggcaggtaga gctggttgac
     1201 cgtggtgacg tcgtccgccg ccgccgggtc ggcgcggtcg agcccgagcg ggttccgctg
     1261 caggtcgatg aggtggaggg cggtgcggcc gaagcggccg tccgggccgg gatagtggac
     1321 gttccaggcg aactgctcgg agacgacgcg caccaccacc gcgtcgcgct ccggcgggat
     1381 ccgctcgacg cgggcggccc agagcggcat cgacacgccg accagcagcg ccccctcggc
     1441 caccaccacc gcggcctcga gccaggccgg gaagcggccg gtgaggccgg cgtggctcgc
     1501 cgccgggtgg cgcgagcgcc ggaagcgcca gagggcggtc aggaagaagg cgaggccgat
     1561 ggcgaacacg gccgccatca gcaggtgcac ccaggagagc gcggcgtcga tctgcgcccc
     1621 gtgcgccgcg gcgttcggcg ggagccagcg gtccatcagg cggcctgctc cactccgtcg
     1681 tggcccgcct gccgcgcccg cttcctcagg tagaggaaga acctcacgaa gcccgccagc
     1741 accgccaggg tgatcccgag cagcaggaag accccgaggc gggcgccgct gatcagcggc
     1801 gcgccggcgt ccgcggcgcc gtagcaggag gtgcaggccg cggcgggcag ggccgcgagg
     1861 aggcccaggg caagcacggc aacggcgaga cggcgggcgg acggggtggc catggcgcgg
     1921 agctccatct agagaatgta gaggacggga aagaggacga tccagaccag gtcgacgaaa
     1981 aaccagtaca gcgccgcgtt ctccacccgc agggcgaagt ggcgggtatc gctcccctgg
     2041 agccgcgcgc ccggtcccca taggtagccg ttgaccgcca gcccgccgag aacgtgcagc
     2101 gcgtgcagcc cggtcagggt gaagacgagc gccaggaagg tgttggtgga cggcagcagc
     2161 ccacgctcga tcctggccgc ccactccacg gactcgagga tcaggaacag cccgccggcg
     2221 agcaccgtcc cgcccatgaa ccggcggaac ccgcggaggt ccccgccccg gatcgcccgc
     2281 cgggcgaggg ccatggtcac cccggaggcg atcagcacca gggtgttgac ggtggcgagc
     2341 ggcacgtcga ggagcgcggc cgggcgcggc cagctcggag cgccggcgcg cagcatcacg
     2401 tagctggcga acagggcgcc gaagagcatg acctcggagg ccaggaacag ccagacggcg
     2461 atccggccgt tggtgagccc ggtgtccggc cgctccgcgg aggtgtaggg gatctcgctg
     2521 gccatcccct accccgcctt cggcgccggc tggaactgcg gggtgaagtc cccggggtct
     2581 cccggcacgc tgtactcgta gggcccgcgg tgggctacgg gcgcggtggt gaagttgccg
     2641 ggcggcggcg gcgacggggc cgaccactcc agggtggtcg cctcccaggg gttgtccccc
     2701 gccttccggc cgcggcggag gctgtagaag aaattgaaga cgaacgggat ctgggccagc
     2761 gccaggcacc aggccgagac cgacatgaag acgttccact ggatgacgtc gcgggcgaag
     2821 aggtaggagg cgccgccgtc gtagagccgc cgggagactc ctccgagccc ctggatgaac
     2881 atcggcagga agatgccgtt catgaacagc agcgacggcc agaagtggag ccgggccagg
     2941 cgctcgctca tcatccgccc ggtggccttg gggaaccagt gatagatgcc ggcgaagagg
     3001 gcgaacaggg tcccgggcgc cacgatgtaa tggaagtggc cgatcacgta ataggtgtcg
     3061 tggagcggga tgtcggtcgc cgagagcccg agcggcagcc cggccaggcc gccgagggcg
     3121 aacatcggca ggaaggcgag ggcgaagagc atcggcggcg tgaagcggat cgagccgccc
     3181 cacagcgaca ggagcagcga cgtcagcagc accaccgagg ggatcgagat gatcatggtg
     3241 gtggcctgga agaacaccgc catggtcgtg cccatgccgg tgaggaacat gtggtgggcc
     3301 cagacgagga acgacatgaa gcccaggaac cagacgccgt agaccatcgg ccggtagccg
     3361 gagagcggcc ggcggatgtt gttggcgatc acctcggcga ggatgccgaa agcgggcagg
     3421 atcagcacgt agacctcggg gtgggcgagg aaccagaaca ggtgctgcca gaggagcggg
     3481 ttgccgccgc cggagacgtc gagcggctgg ccggtgacgc tgaggccgct cggcaggaag
     3541 aagctggtgc cggcgaggcg gtccatcagt tgcaggacgg cggcggcctc gagcggcggg
     3601 taggcgagga gcagcaggaa cgccgtcacg agctgggccc agacgaagaa cggcagcctg
     3661 aagaaggaga ggccgggggc gcggagctgc acggtggtga cgatgaagtt gacggcgctc
     3721 agcagcgtcg aggcgccgag gaagagcagg ccgaggagcc acatcgtctg gccgtggccg
     3781 gcgatgacgg agagcggcgg gtaggacgtc cacccgttct gcgggccgcc ccccgggacg
     3841 aagaagctgg cgagcatggt gacgccggca aggagatagg cctgatagct cgcccggttc
     3901 atgcgcggga aggccatgtt gggcgcgccg atctggaggg gcagcacgaa gttgccgaat
     3961 ccgccgacag cgagggggac gacgccgagg aacaccatga tggtgccgtg cagggcgccg
     4021 agctcgttgt aggtctcggg gagcatgaaa ccccccggca tccgcgcctc gccgagcagc
     4081 cgtccgagcc acggcagcgg ccgcccggga taggcgagct gccagcgcat gatcatgacc
     4141 aggcagaacc cgaagaacag gaacagcagc ccggtcaccg catactggat gccgatgacc
     4201 ttgtggtcgg tcgagaacac cgcccgccgc cagaaccccg gacgcgcctg cgatgcagcc
     4261 tcgtccgtcg ccgtctcggg acgagcgggc gcgaccgtgg ccaggggctc gagctgcttg
     4321 gacatgaggg agatcctgcg aggcggggtc actccccggc accgcggggg cgagtcagta
     4381 tagagcaacg cggcgggggg acgcccggcg gtcgccatcc atgagaatgg gtcacgatcc
     4441 cccgttcccg gatcactatc cgaacctccc ggaacactat tcgtgttccc cggatcactc
     4501 tcccccggtc ccggatcact cccggagacg gaaggaacac tccggcgtct tccggaacac
     4561 tcttcctgct cgggggatca ctccgccgcc aggaagagcg cccctcctcg gaggaatcgt
     4621 gtccatcccg gtcgccatag tgatcccgga accttcccga atgatccagg gagcctgctt
     4681 tgtgttccgg aaccggcggc ggagcactcg ttgtgatccc ggaacgctcg ggaacacaca
     4741 gcagactctc gccggcagat ccagaacata cgtcgatgac ttgattgaca atatggcgac
     4801 tcacatcgca gtctctgctc cgatgattga cgcctaagac ctccgaagtg ccttgtcgag
     4861 gtcgagggaa tcgcagcaaa gcgttgagga gagggccgtc cacctgaggc accgtccctc
     4921 aacactccgg gttcattgac aactgaaatc gtgacggggc gccgccgagc ccaaggccgc
     4981 gcccctgagg atgcctccgt ccgacaggag gacaaccggg tggtggaggg ccgatccctc
     5041 cgcctcgccc ggatggcgtg cgtctgctcc gccgggaatg aggactccgg gcggtagctt
     5101 ccccatccag gggcgccatg agggggtggc ttcctccccc cctgggagga tgggcggtag
     5161 ggagtggggg aggactgagg tggggggttt ctttttgggc gagcgtcggg aaaggaagcg
     5221 tcaccggcag gccgagccta ccggcgacgc ctatgtatat gtataaggat gtcctacccg
     5281 gccgccgccc cggcggtgtt ctcggccctg acctccgcca cccgctcgtc gaggatggcg
     5341 ccacaccgct cgagggtgac cccacccgcc gcctccagca cgtacgccag acacaccggc
     5401 gcctggagga gatggatgac gacgttcaac gcgtcgaccg ccttcttgtc gaagagctga
     5461 aggaagccga caggggggcg gtggccgttg tcactcccca tcgccacgtc ttcccccacc
     5521 ggcaagaaca acgggatacg cccacaggac gaggcccgcg gtctggctgc gcgagaagcc
     5581 gtccaccgct aattcgcact tccggccatt acgatgggtg gtgcctaatc tacccttcaa
     5641 ccgaatcacg ggatcgctga cctatgctaa cctaatcggc tgtctgctga actaccgaag
     5701 accagatcgc catgtgggct gctctgacgg ccgccgccgc catcgccgaa atcctatccc
     5761 tccttcttgc ctggggtgct taccgccgtc ggcggaacct caagaaggag ttggcagagc
     5821 tgcgcttggt tccggaggaa gagcgcgccc ggctgctcga aaggaagctc gaccgctttg
     5881 gggtcgcggc ggcgggactt tccaagaagg acagcttctt gatcatccag cagacaatcg
     5941 agcggcaggc ggaacggttc cggcgcgcct tgtgggcggg agtctccgcc tgtctaatct
     6001 tcgccgcgct caccgcgtat gcgtggaccc tcgatcagtc ccccgatctc tatcgcttgc
     6061 gggtgacggt tctcgcaccc gacggccgcc cgtccaacaa cgcctttgtc agtacctcgg
     6121 tgggcggcga gatcttgaag gcgcaccaag gttgggagat cttgctccca cgcaaccgcc
     6181 gaggtcaggc cttcactgtc ttcgccagct atcctgaggc ctttctccag ggtcgtcagg
     6241 aggtggcgct gggtgacgac ttcgagccgc cagccatcga aatccaactc tcccgccggg
     6301 aggtcgaggt ccgaggcacc gtgatcgacc cgcaagggca tggggtagct ggcgccaccg
     6361 tgagcgttgt gggctttgag caggaatccg tcgggaccga tacatcggga aatttccgtc
     6421 tgcaggcgca cgccgccgac cggcaaacgg tgcggctgcg agtggtcaag gatgggagat
     6481 cgagcgagtt cgaggcacgc gccggagatc ttcctgcgca gcagttggta tgggaggagc
     6541 catgaagaag atcattcctt ttcttctctg ggccgtcatg tggtctgtca ccgggccgac
     6601 cgccgcggag gatcgcatcc tgcgggtgca ggtggttgac gtcgcaggcg ttccgcaagc
     6661 caggctgctc atcggtctgg ccggtgaagg cacctttcag tcgaccgatc agcaaggaaa
     6721 tgcgcgcctc ccgcttccgg cgcaggcccg ggtgggcgat ccggtcacgc tgcggctcag
     6781 caacaaagcc ctggtcttca tcagcccgtc cgaaggccgg acccatatcc cctcgttcga
     6841 tgagaagcca ggaaactttg tcaccgccct ggtgggaaag ctgggagagc tgacccttct
     6901 aaaaagcgga gatgtgctgc gcagctttct caaccaagtg ctcggcgagg tggagcggct
     6961 ccaggagcgg agcacagaag cgaccgcgac gcagttacgc acggtgctcg acgagcgggc
     7021 cggtggcttc ggtctcaatg ccgaggactt cgaccgtgcc atccgcgctt ggctcgaagc
     7081 ccccgtcggc ttagacgaca aacgcctcgc agcgcgttat cttgagctat acccggagcc
     7141 cgtctcgcag aacagctacg gttctgtggt ggtcggcgaa ggcgccagca tgaaggattc
     7201 cctgatcgtc accaacccac aaaactcacc catcaccatc cagaaccggc gccacgacgt
     7261 ggattcggca ctttccgtga tcgcccgtgc agtcgaggct cgccccaccg gcgacattgg
     7321 tcaaatcgcc gccgccgaat tcctggcagg ccaggaaggc ccggggctta aaggtatgga
     7381 tttggccgga ctgtcattta ccggcgccac cttggaaacg gcaaagctat ccgaggccat
     7441 tctcgacggc gcagattttt ctcatgcaaa tctgcagaag gccgatctct cctccgcgaa
     7501 gctcggcttc tcaaccctgc aggaagcaac actcgcagaa gctcttctgg aaggtacccg
     7561 cttctacttt gtctcggcga ccaaggcgat cttcgacaac gctcgcgccg ctggtagcaa
     7621 ttggacgggg gccgatgttc gcggcgccag cttccgcggc gcagatctgc gcggtgcgag
     7681 cttcgcgttt accgacttga ccggagccga tttcacggga gccgacctca ccgagacgct
     7741 ctttatttca gcgattctga cggacgtcag gttcgacggg gctaccgtcc gcaatacgga
     7801 cgctaccgcc gcagtgggcg acgtctcgat ctttaccccg gtgcagcagc aggagctgtg
     7861 tgcgacaggt ctcggccaag gaagcggcca ggtcgaactg gtgcgagtcg tacctagcac
     7921 gcgcttcagc agcggctccg actacgatcg gctcacttac ggcaatctcc cggcgctcga
     7981 agccctgcac ctgctcgacc cttgtacctt gcggatccat cgacccgcag ggttcgagac
     8041 ggtctggagc gcaggcaaca acgaatacgt caagacggcg tacgccttcc attatccgtc
     8101 cgacctgctg gacaaaggct cgcgcagtgg aaagttcgcc gcgcgcctga aggagcagtt
     8161 gaaacggttg gatgaggcca tggccagggg cccgttcatc gctgtacgcg gcaagactca
     8221 cgcggcgctg gcccgcgcac tgaaagcgaa tctgggaaag accgctcctc tggcgcctct
     8281 cgacctcttt gatgaggaga ccatctttct actaaccctt cgcttcctgc ccaaggaggc
     8341 ggacaagatc gactggcacg gccgtgccca gaatcggatg agagacgaac aataccgcaa
     8401 ggcgcatccg gacgacggcc cttccatctg gccgatcttc tttccggagg gttttagcag
     8461 caaccagcta ggcgctgagc atgtagagct ctatcgccaa tggacgcaag gccgcttgaa
     8521 acgcctgccg ctagagttca cgctcactac gcgcttcgag ggtggtgcac ggtctcgatc
     8581 acgccgcacg ccgcagcccg aaaccgcagg cgctccggtg cgtgcggccc tgctcgcgag
     8641 cttcggcgag cccttgcctg atggtctcct cggcaaggta ggcggcggtg ccgcggatgg
     8701 attcgcattt gacgtgcatg gagaagacta cgcactggtc ttcgcccaat cacgagccgc
     8761 ctacgtcctc gatatccccg gcgagattgc cgaggagctc gcgacgcaga atccccaggg
     8821 cgaagtccgt attcgctttc aaggagccga gatcgttgag ctagaatcgc gccggtatcc
     8881 cgagcaacaa gaaaacgatc gtatgaccgt gttccgcgtc gagccgatct ccataaggtt
     8941 ggtggggcgg caggggctca ccttcccaag ctctcccgcg gaggccgaga agctccgaat
     9001 catccctcca aaacaggccg agaaacgaat cgagttccgg cgcaggctct gtacctacta
     9061 caccgttccg gcctcggccc tcgccaatct gcgcgacaaa gaggccccgg acctcccttc
     9121 gatccaaagc atgctttccg acgacccttc ggccgagtcg ctcaagaccc aggagtatct
     9181 tctgcggcaa ctgaacgata atcccggcta cgctgacatc ctagccgact attttattca
     9241 gacaatcgcg tcgggagagt ccaaggaaga tgaatactta ggtccgatct acaagcgtaa
     9301 gctcggagaa tttgagcccc tgaagccacc agcgtcctgt agatagagcg gtccccagcg
     9361 ctcatggggt gccctactca tgaggtgcgt agtggctggc tggctggcta cctactcgta
     9421 gtgaagagaa ccttaagtta ccctctccaa gccgcttcta cggcgtaatt ggccgccggt
     9481 cgcggtccaa ggggtggcct cagcgggccg gaagggtcga aaaagggagg ctcgagggga
     9541 gatggcgagg cttggaagag cctcccagtg gcgtttgtag ggcggcaaca ggaccggcag
     9601 gcacacctct cctggtcgag gtcatcggag cttacccgcc gaccagtttg cggcctcgtc
     9661 cgccgcctcg ctcagccgcg aacggctagc cgaaggccgg ccggacgaag ggcagcgccg
     9721 gagggaagct cccctccggg aactccaccg tccggtcgcc ggccttcagc ttctcagcgg
     9781 cgtcccggaa agccgccagg aagcgactgt aggcctcccg cagattcagg taagccttct
     9841 tgctcgccgt gtggaacagc ggcgccggcg acttcttggt ccggttcggc cgggtatgcg
     9901 gctgctgcct ccggaccgcg tccgctccca tgggctcgag ccccttcgcc tcccgctccg
     9961 cccgggcctc ctccacgact tcggccacca agtcagccat ccgctgccgg tattcctctg
    10021 ccggcaagtg cttccagcag ggcagcggat cgagagtcag cgtctcgacg gtcgcgaact
    10081 ggcgcggcgc gggctctttg cccctcaacc tgtaccggta ctcctgggtt cggtcgtacc
    10141 agaccccttc caggggatcg ccggtcaaca gggcgttcac cgcgtgggcg cccggccact
    10201 cccggggatc ggcgacaagg ccctccttgg cgccatggga gaggatgtac ttgaggcgcg
    10261 ccacctgggc cgcctcctcg tcgctcacca cgatgtgctg gtagcgccgg ccccagaact
    10321 tctccttcca gtcggccaga cgccccacct ccttggcgag gttgccgttc aggtaaccca
    10381 tgaactcggc catgcggccg gcgtcctcca cgtggatcag gaggtggtaa tggttggagg
    10441 tgaaggcgaa gcagatcaac tggaccgggt acaaccgttt ggcccgcgcc aggactccga
    10501 ggacgatctc ccggacgtag gcacgagggg cgagcagcag acggctctgg acggtgcggc
    10561 aggtcacctc gacgagcgac ccgccttctg ggacatgacg gatttttctc ggcatgcccc
    10621 tacaagacgg aaagcgccgg aaaaatcgct caactttagt gcatccgcga ctctctatgg
    10681 atcacgaagg catcagcacg ggccgatcga tgccccggaa gtcaccgtca atggccagaa
    10741 tggggcagtc ttccacagcg gccaaggcat aggcgaagca atccccgaga ttcagcggca
    10801 accgaagacg ggcctcggct gcgatctggg cctgccgcac atcaggtgga atgaagcgga
    10861 tgccgctgct caacagctcc gcctcgatcc gctcgtacag ccgcggctgc cggtcacgaa
    10921 tccgtatcaa cgcctctgtg aggttgactg tgctcatcct cagatcggga gcgtgctcct
    10981 ccagccgagc gacggcccac tcggcttgag gctcctcgaa gaagatcgcg agcagcacgg
    11041 acgtgtcgat caccattgcg tcagccccaa agatcgtcgt ccgagcggaa ccgcgggctc
    11101 ggattctccg gctcacggtg ccccaagccc agccatttcg agaactccgt cggcgcccgg
    11161 cgccgacgct ctcgctctgc agccgacaga acagcccacc ggactgcctc ggacttcgta
    11221 cggatctggc gaagtcgcat cagctcgtcc aaagcctctt cgaactcctc ggtcagatga
    11281 agattgagct gtggcatcat ccctcctgta cagaatgtat acagaaggcc gagagagggg
    11341 tcaagtcggc gaggacgcaa cctcccactc cggccgcagc agccccagga ttaccgagtc
    11401 ctggacctcg ccgttggtgt ggtagcgctc ccgcagatac ccctcgcgcc ggaatcccag
    11461 acgctccagc aggcggagcg agggctcgtt acgggggtcg gcgtccgcct ccagccggtg
    11521 gatccccagg ctctggaagg cgaaggaaag gaggagggcc acggcctcgg ccgcgaagcc
    11581 cttcccctga tgggcccgcc ccagggcgaa gccgatctcg gcccgacgat ggcgggcttc
    11641 ccagcggaaa agggtgcacg tccccagcac ggcgccgccg ccacgcgggg cgatccccca
    11701 ttggaagagg gtccgcgccc ggaaatggtc atggatctcc cggaggagcc cggcggcggc
    11761 ggcgagatcg cccaggggcg gcgagtccca gtaccgcatc acctccgggt ccgaaaagat
    11821 ctcgaagagg gccgggacgt cctcctcccc cagccaccgc agctccaccc gcggtccggt
    11881 gagcgtcggc agccggtccc cccagtcgta gtcagctccc tgtgaggaca tcctcgcggt
    11941 cccctcctcc gctcagtccg ccacccacag ccgccgccac ccttcgtggc cgaggcggcg
    12001 gagggtctcg atgttcttct cgtagatccg ctcggtctcg gggaaggacg ccaccgcgcg
    12061 ctcgacgctc gcctcgcgca gcaggtggag gatcggatag ggggagcggt tggtgtagtt
    12121 ctcgatgtcc tccggcgcgg tgccggcaaa ctggtaacgc ggatggaagc tggcgacctg
    12181 gatctccccc tcgagcccga gctccgcgac cgccgtgtcg gcgacgtcca ggaaatcgtt
    12241 gtagtccagg aagtccacca gcaccccggg atggatcagc agcgtggtct cgacctcctc
    12301 cgggtccgcc gccgcgatgg cccggagctc gatgacgagg tgctccagca ggtcctcggg
    12361 cgtcgtcgcc tcgctgaccg tgtagcggat ctggtccttg agatggacgg acttcgcgaa
    12421 cgggcagagg tcgagaccga tgacggccct ctcgagccag gtcttcgtgg ctttgacaat
    12481 gtcttcggca gatggaatcg gtgtcatggc tgggccgagt atactgcgcc ttggcgcaaa
    12541 tggcactaca gttgcaattt cctcatcccg ggaggagaaa catggcgcgc tacaagatgc
    12601 ggcagcggct gatcgcgatc ggcgaggact tcgacatcca ggacgagcag ggccgtccga
    12661 tttatcacgt ggacggcaag gtcctgcgta tccgcgagac cttcgtgatc caggacctgt
    12721 cgggcaacga ggtggcgacg atccgcgaga agaagatcgc gctgcgcgac accatgaaca
    12781 tcctgcgcgg cggcgaggtc gtcgcgacca tccgcaaggc ctggatcacc ccgctgcgcg
    12841 acaagttcgc catcgacgtc cagaacggcc cggacatgac ggcccagggc aacatcctgg
    12901 accacgaata cgagatccgc cagggcggcg agacgatcgc cagggtgtcg aaacactggt
    12961 tcacgatccg cgacacctat gggatcgaaa tagcccccgg ccacgacgac ggcctcatcc
    13021 tcgccatggc ggtcgcgatc gacgagatgg cgcacgatcc ggacgaaggc aagggctaga
    13081 gccccggtgg accgctcttg tcgttcgatc tctggttcct ggtactcggc ggcctgctgg
    13141 tcgtcatcgc cctgatcgcg tcggtcgtcc ggcacctgcc gctcaccacg accgtcctct
    13201 atctattagt ggggctcggc ctgggcgcgt tcgggatcgg cgccatccgg ctcgatccgt
    13261 tgcgccacgc ggccttactg gagcgggtga ccgagctcgc ggtcatcgtc tcgctgttca
    13321 cggcgggtct caagatccgg ctacccgtcc atgatcccct ctggcggctg ccgttccgtc
    13381 tcgcctccct ctccatggtg gtcaccgtcg ggctgatcac gctcgtcggg acctggggcc
    13441 tgggcctccc cctcggcgcg gcggtgctcc tcggcgccat cctcgccccg accgacccgg
    13501 tgctcgcctc ggaggtgcag ctcgcggccg cgggggaccg gaaccgcctg cgcttcggcc
    13561 tcaccggcga ggcggggctg aacgacggca cggcgttccc cttcgtcatg ctcggcctgg
    13621 gtctcctcgg agcccacgag atcggtgaat ggggatggcg ctggcttgcg gtggacgtcg
    13681 tctgggcggt cgccgccggg ctcggaatcg gcctcgtcct gggcaccctg gtggggcggc
    13741 tcgtcctctt cctgcgcaaa aagcacaagg aggcgttcgg cctcgacgac ttcctggccc
    13801 tggggctgat cggcctctcc tacggcgcgg cgctccttct ccactcctac ggcttcctcg
    13861 ccgtcttcgc ggcggggctc gccctacgcc gcatcgaacg caaggcatcc ccggatgagg
    13921 aggacccggg cgccgcccag gagaacctcc gcgccatggc cgccgccggc aaggaggagg
    13981 agatcgccac cgatcccgag aaggcgcccg cctacatggc ccaggcggtg ctcggcttca
    14041 acgagcaggt ggagcgcatc ttcgagatcg gggtcgtgct cctcgtcggc gccatgctct
    14101 cctggcgctt cctgcccggc tccgccctct ggttcgtccc cgtccttttc ctcgtgatcc
    14161 gcccgctggc cgtcctgacg ggccttgccg gcgctcggct gcccgctctg gacagggggc
    14221 tgatgtcctg gttcggcatc cgtggcatcg gctcgatcta ttacctcatg tatgccatcg
    14281 agcacggcct cgaccccgcc ctcgccgagc cgctgaccgc cgtcaccctg accaccgtcg
    14341 ccgcctcgat tctcatccac ggcatctcgg tcacgccgct catgaagcac tatgagaaac
    14401 ggctccgcaa gcggggggag gctcccgccg ccacctagat cagcgccacc tagaccagcc
    14461 cgcgggcgga gagctcggat ttcaggtagg cgtagtagat cggcgcggcg atcaggccgg
    14521 ggatgccgaa agccgcctcc atcgccagca tcaccagcag cagctcccag gcgcgggcct
    14581 ggatctcgcc gccgacgatc cgcgcgttga agaagtactc gagcttgtgg atcaccacca
    14641 ggaaggccag cgagccggcc gccacgccca gcgagtacga gaagctgacg acgacgatca
    14701 ccgtgttcga gatcaggttg ccgatcaccg gcagcaggcc ggccaggaag gtgatggcga
    14761 tcagcgtctt ggtgaacggc agccggatcc cgagcacggg gagcaccacc acgagatagg
    14821 tcgcggtcag cagcgtgttg agcgccgaga tgcggacctg ggcgaagacg atgcgccgga
    14881 acgcctcccc cagccggccg gcctgctcgg tcagcgccga agccaggggg gcatggcgct
    14941 ggacccgccg cgtctcgttg agcgacacca tggcgccgat gatcatgccg atcagcgcgt
    15001 ggaccagcgc ccggccgacc tcggcgccga acatctgcag ctccccggcg tgctcgcgca
    15061 gccaggccac ggcctcgttc ttgagctcct cggcgttggc cgggatctcg cgggcgagcc
    15121 agagcggcag gtgctcccgc gagccctcga cgatgtcggc catcttcgcc agcagcgccg
    15181 gcaggttccc cgggccgctc cggaagaagg cgatgaggcc gaagatcgag aaggtcaggc
    15241 ccgcgacgat gacgaccgcc agcagggtga ccgccacgta cttcctcgcg tctgtcctgc
    15301 cgtctggagc cccgctgccg aggaagcgca gccggcgcac caggatgtgg accagctcgt
    15361 agaccgccag cccgccaatg agcgccgcca gcaggcgcag atggacgacg aggagcaggc
    15421 cggcccccat caagatccag gaggcgattt cggaggggga cggggaggga cggcggtgga
    15481 ggtccggcgc gggttcggcg gtcatgtcag gggcctcgaa ggcacgccga ttctcgcaca
    15541 gcgttatact gccgccgaag ggggactacc tcatgccgaa gcgcaacgtc cgactcacca
    15601 ccccgctcgt ccgtgagaac ggcgctttgc gtcccgccac ctgggacgag gcgctcgacc
    15661 gggcggcgca ggggttccgg tcggctgtcg aggcgaaggg gccgcagacg ttcggcctct
    15721 tcagttgctc caagaccacc aacgagatga actatctcgc ccagaagttc gcccgggcgg
    15781 tggtcggcag caacaacatc gacagctgca accgcacgtg acacgccccc agcgtcgtcg
    15841 gtctggcgac ggtcttcgga atgggcggcg gcacgacctc ctatagggag gtcgaggaga
    15901 cggacctcgt catcctgtgg ggcagcaacg cccgggagac gcatccgatc ttcttccatc
    15961 acgtgctgaa ggcgatccac aacggggcgc ggctctatgc cgtggacccc cggcggactt
    16021 cgtccgccga ctgggcgacc ggcggccacc tccccctgca cgtcggcacc gacgtcgcgc
    16081 tggccaacgc cgtggcgcgc gagatcctcg ccgccggcct cgagaaccgc agcttcatcg
    16141 acaacgcgac cagcggcttc gaggagttcc ggacccacgt cgagccctat accctcgagt
    16201 acgccgaggc ggtcaccggc gtcccggcgg cgacgatccg cgacctcgcc cacgcctatg
    16261 cccgggcgga ccgcgccgtg ctctgctgga ccctcggcat caccgagcac cacaacgcgg
    16321 tggacaacgt ccgcgcgctg atcaacctcg ccctgctcac cggccacgtc ggccgctggg
    16381 gctccgggct caacccgctg cgcggccaga acaacgtgca ggggggcggc gacatgggcg
    16441 ccctccccga ccgcctgccc ggcttccagc acgtggagaa cgacgagctg cgcgcgaact
    16501 tcgagcgcgt ctggggcgtg ccgatccccc cgaagcgcgg ctggcacctc tccgggatgt
    16561 tc
//
LOCUS       DASPJI010000587         3202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_335259, whole genome shotgun sequence.
ACCESSION   DASPJI010000587 DASPJI010000000
VERSION     DASPJI010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335259"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VEL74_16450"
     CDS             complement(<1..733)
                     /locus_tag="VEL74_16450"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HYG64168.1"
                     /translation="MTGASISSDSGIQAVGDINYGTHFCQFYRTRQDLRDVLVPYFVA
                     GLRNEEYCMWVTSEPFGAEEAEAMMREAMPDFSDRLARGQIEIWDYMDWYLANGGRPA
                     QVLQGWIDREKRALDRGYTGLRLTGNTFWVERGVWDEFVAYESEVNRAFGQYRIIGLC
                     TYCLDRCRAEDVLDVCRNHQFALTRREGEWELIESSSLKIAKEELARVNLGLEQRVAE
                     RTRALEEALAGRDEFLAMLGHELRNP"
     gene            complement(770..1642)
                     /locus_tag="VEL74_16455"
     CDS             complement(770..1642)
                     /locus_tag="VEL74_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HYG64169.1"
                     /translation="MPGRRRYTGRPMNGIELRHLRYFLAVAEELSFSRAAERLGMAQP
                     PLSQQIQRLETLVGHELFERRPRVRLTPAGEAFLAAARRALAQVEEGVEASHRAARGE
                     VGTVTVGFAASMILSVVPGAVRAFRRRFPGVDLQLRELSSAAQLAALREGRIDVAFVR
                     ESPFSDRELGCERLREEPFVHFPRPVAPGLYDLILALCQGAGFKPRVVQEAQEWLTIV
                     GLVEAGMGVSLVPESFRKLRWGKVVYLPLDRATARSTILLCWRATGERTAEHPAVQAF
                     LQTAREAWGREGVQ"
     gene            1666..>3202
                     /locus_tag="VEL74_16460"
     CDS             1666..>3202
                     /locus_tag="VEL74_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG64170.1"
                     /translation="MTTWAHRTDDHEERERLAARLAEQTEILETVNRIGQLLSAELDL
                     QKLVQAVTDAATELCGAQFGSFFYNVLNEEGASYMLYTLSGVPREAFASFPMPRATTL
                     FGPTFRGEASIRIDNVKADPRYGNNPPHHGMPKGHLPVTSYLAVPVVSRSGEVLGGLF
                     FGHSEAGAFTDRHTRIVEGLAAQTAIAMDNARLYQSAQQTVRERDALLASEQAARQEA
                     EAASRAKDEFLGLLSHELRNPLNAILGWTRMMATGQLDDGLSQRAIETIERNAKLQAR
                     LIDDMLDVSRIVSGKLRLDAQPVDLTAVINAALDTVRPAAEAKDIRLQSVLDFGAGAV
                     LGDPVRLQQVVWNLLSNAIKFTPKHGRVQVQLERVNSHLEIIVSDTGPGIDEDFLPHV
                     FERFRQADSSASKKHGGLGLGLAIVRHLIEMHGGTVEANNRQDRQGAIFVVLLPLMAV
                     RPSAAPAATAETTAARVHPSSSGSVPFDCPPELDGIRVLAVDDEPDARHLLTTVLEQC
                     GAEV"
BASE COUNT          494 a         1177 c         1091 g          440 t
ORIGIN      
        1 gcgggttgcg cagctcgtgg ccgagcatgg cgaggaactc gtcgcgcccc gcgagcgcct
       61 cctccagggc gcgcgtccgc tccgccaccc gctgctcgag gcccaggttg acccgggcca
      121 gctcctcctt ggcgatcttc agggaggagc tctcgatcag ctcccactcc ccctcgcgcc
      181 gcgtcagggc aaactggtgg ttgcggcaga cgtcgaggac gtcctcggcc cggcagcggt
      241 cgaggcaata ggtgcagagg ccgatgatcc ggtactggcc aaaggcgcgg ttgacctcgg
      301 actcataggc cacgaactcg tcccagacgc cgcgctcgac ccagaaggtg ttgccggtga
      361 ggcggaggcc cgtgtagccc cggtcgaggg cgcgcttctc gcggtcgatc cacccctgga
      421 ggacctgggc cggccggccg ccgttggcca ggtaccagtc catgtagtcc cagatctcga
      481 tctggccccg ggcgaggcgg tcggagaagt ccggcatggc ctcccgcatc atcgcctccg
      541 cctcctcggc gccgaacggc tccgaggtca cccacatgca gtactcttca ttgcgcagcc
      601 ccgccacgaa gtagggcacg aggacgtccc tgaggtcctg ccgggtgcgg tagaactggc
      661 agaagtgggt gccgtagttg atgtcgccga cggcctggat accgctgtcg ctgctgatgg
      721 atgctcccgt catcgccgct cctccgacgg gcacacgcac cgccgaaagt cattgtacgc
      781 cttcgcggcc ccacgcctcc cgcgccgtct gcaggaaagc ctgaaccgcc gggtgctccg
      841 ccgtccgctc tcccgtggca cgccagcaga gaaggatggt ggagcgggcg gtggcgcggt
      901 cgagcggcag gtagaccacc ttgccccagc gcagcttgcg gaacgactcc ggcaccaggg
      961 agacgcccat gccggcctcg accagcccga cgatggtcag ccactcctgc gcctcctgca
     1021 ccacccgcgg cttgaagccg gccccctggc agagcgccag gatcaggtcg tacagcccgg
     1081 gggcgaccgg gcgcgggaag tggacgaacg gctcctcgcg cagccgttcg cagcccagct
     1141 cgcgatcgga gaagggggac tcgcggacga acgcgacgtc gatgcgtccc tcgcgcagcg
     1201 ccgcgagctg ggcggccgag gacagctcgc ggagctgcag gtcgacgccg gggaagcgcc
     1261 gccggaaggc gcggacggcg ccggggacga cggagaggat catcgacgcg gcgaacccga
     1321 ccgtcaccgt ccccacctcc ccgcgcgccg cccggtggct cgcctccacc ccctcctcca
     1381 cctgagcgag ggcgcgccgg gcggcggcca ggaacgcctc gcccgccggg gtcagccgca
     1441 cccgcggccg gcgctcgaac agctcgtggc ccaccagcgt ctccaggcgc tggatctgct
     1501 ggctgagcgg cggctgcgcc atgccgagcc gctccgccgc ccggctgaag ctcagctcct
     1561 cggccaccgc caggaagtag cgcagatggc ggagctcgat gccgttcatc ggccgaccag
     1621 tatagcggcg gcggccgggc aaggatcttg ctccaaaggc cgccgatgac gacttgggcc
     1681 caccggaccg acgaccatga ggagcgcgag cgcctggcgg ccaggctcgc ggagcagacc
     1741 gagatcctcg agacggtcaa ccgcatcggc cagctcctct ccgccgagct cgacctgcag
     1801 aagctcgtgc aggcggtgac cgacgccgcc accgagctct gcggcgcgca gttcggctcg
     1861 ttcttctaca acgtgctgaa cgaggagggc gcttcctaca tgctctatac gctgtcgggg
     1921 gtgccgcgcg aggcgttcgc gagcttcccg atgccgcgcg ccaccacgct cttcgggccg
     1981 accttccgcg gcgaggcgtc gattcggatc gacaacgtca aggccgaccc gcgctacggc
     2041 aacaatccgc cccaccatgg gatgcccaaa ggccatctgc cggtgacgag ctatctcgcc
     2101 gtccctgtgg tgtcccgttc gggcgaggtc ctgggcgggc tcttcttcgg ccactcggag
     2161 gcgggcgcct tcaccgaccg ccacacccgg atcgtcgagg ggctcgccgc gcagaccgcc
     2221 atcgccatgg acaacgcgcg gctgtaccag agcgcccagc agacggtccg ggagcgcgat
     2281 gccctgctcg ccagcgagca ggcggcgcgc caggaggccg aggccgccag ccgcgccaag
     2341 gacgagttcc tgggcctgct ctcgcacgag ctgcgcaacc cgttgaacgc catcctcggc
     2401 tggacccgga tgatggccac ggggcagctc gacgacggcc tgtcccaacg cgccatcgag
     2461 acgatcgagc gcaacgccaa gctgcaggcg cggctgatcg acgacatgct ggacgtgtcg
     2521 cggatcgtct ccggcaagct ccgtctcgat gcccagcccg tcgatctgac cgccgtgatc
     2581 aacgccgccc tggacaccgt gcgcccggcg gcggaggcca aggacatccg gctccagtcg
     2641 gtgctcgact tcggcgccgg cgccgtcctc ggcgatccgg tgcgcctgca gcaggtggtc
     2701 tggaacctgc tctcgaacgc gatcaagttc accccgaagc acggccgggt gcaggtgcag
     2761 ctcgaacgcg tcaactcgca cctcgagatc atcgtcagcg acaccggccc gggcatcgac
     2821 gaagacttcc tcccccatgt cttcgaacgc ttccggcagg cggacagctc ggcgagcaag
     2881 aagcacggcg ggctggggct ggggctcgcc atcgtgcgcc atctcatcga gatgcatggc
     2941 gggaccgtgg aggcgaacaa ccggcaggac cggcaggggg cgatcttcgt ggtgttgttg
     3001 ccgctgatgg cggtccgccc gtccgccgcg ccggcggcca cggcggagac gacggcggcg
     3061 cgcgtccatc cttcctcgag cggcagcgtg cccttcgact gcccgccgga gctcgacggc
     3121 atccgggtgc tggcggtcga cgacgagccc gacgcccgcc atctcctcac caccgtcctc
     3181 gagcagtgcg gcgccgaggt ca
//
LOCUS       DASPJI010000588         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_38133, whole genome shotgun sequence.
ACCESSION   DASPJI010000588 DASPJI010000000
VERSION     DASPJI010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38133"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VEL74_16465"
     CDS             complement(<1..474)
                     /locus_tag="VEL74_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64171.1"
                     /translation="MLSWSCRRFRAGFTPGRPETDHPHRFRCPACGEYAAAMEKAFTA
                     GRLVSISDRLRQRLESIPSSGAARVVRFPLPRVPLPDRLRERLRDIPRQAEAPRPPAW
                     VLHPRYAVAASYLLAVLLTLALGDSVERGREAVESLAHGLAAPIQRAESAGRERLE"
     gene            complement(468..1082)
                     /locus_tag="VEL74_16470"
     CDS             complement(468..1082)
                     /locus_tag="VEL74_16470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYG64172.1"
                     /translation="MDAPLPTPDLLLARRAAAGQPQAWDELIERHGGRLFNLALQFSA
                     SVEEAEDLTQEIFLRLYQSLRLYRGDCPLLGWALRLSRNLCIDHWRRVRRERLATAVS
                     EEILLQMPATDDPRAQAQARQELRAVHEALTAMPEELAEVVLLRDLQGWSVEETAAYL
                     EVPAGTVKSRLHRARLELAEKVTDRLGGRPETGPAAVLGGAGSC"
     gene            complement(1142..1372)
                     /locus_tag="VEL74_16475"
     CDS             complement(1142..1372)
                     /locus_tag="VEL74_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017746903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HYG64173.1"
                     /translation="MPRLTPLHWRVLEKVFLAVGFTLARQEGSHRSYVRPGTLRPVVI
                     PTYDEVPISIIRNNLKTAGISREELFEILEKL"
     gene            complement(1374..1643)
                     /locus_tag="VEL74_16480"
     CDS             complement(1374..1643)
                     /locus_tag="VEL74_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017746902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64174.1"
                     /translation="MHLDVTVHIWKEGGQYVAHAMPLDVMSSGATPELARQAVDEAVR
                     LFLLTATDAGTLEEVLEESGYELHGDRWVSPSFVSVEKHDLALAV"
     gene            complement(1648..2322)
                     /locus_tag="VEL74_16485"
     CDS             complement(1648..2322)
                     /locus_tag="VEL74_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64175.1"
                     /translation="MASHPSPATPPRARPLAVQITGVLLFLFIPLVLYLYVGHPEPVW
                     LSLLAGVALMVGHRRLARPYMTRVLPVKCVWCNRVLAPDAPSEVLEVRAGKETLRARC
                     CPGHRRGAAKYFAILDAWRWPLRLGIFVPLLLLLGALLLTALGRDVPLATVTAWFRLL
                     IGITVNLAAFGYLLGLLAPERSPVEVPFPVHNFFLLGVRALLWVFRFVGLWWIWKGAS
                     FLLGVE"
     gene            complement(2333..>2532)
                     /locus_tag="VEL74_16490"
     CDS             complement(2333..>2532)
                     /locus_tag="VEL74_16490"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HYG64176.1"
                     /translation="QYRSAIFFHTPEQERAARESKETLDKSGRFNRPIVTQVEPAQIF
                     YRAEEYHQQYLEKRGLASCHI"
BASE COUNT          417 a          876 c          912 g          327 t
ORIGIN      
        1 ttcgaggcgc tcccgcccgg cggactcagc gcgctggatc ggcgcagcca gcccgtgagc
       61 gaggctctcc acggcctcac gcccccgctc cacggagtcg cccagcgcga gggtcagcag
      121 aaccgccagc aggtagctgg ccgccacggc gtacctcgga tggaggaccc aggcgggcgg
      181 ccgcggcgct tcggcctggc ggggaatgtc gcgcagccgc tcccgcagcc ggtccggcag
      241 cggcacccgg ggcaggggga aacgcaccac gcgcgccgcg ccggaggagg ggatcgattc
      301 cagccgttgg cgcagccggt cggagatgga caccagccgc ccggcggtga acgccttctc
      361 catggccgcg gcgtactcgc cgcaggccgg gcagcggaac cggtgcggat ggtcggtctc
      421 cggccggccc ggcgtgaagc cggcccggaa gcggcggcag ctccagctca gcatgaaccc
      481 gcccctccca gcacggcagc cggtccggtt tcggggcgcc cgcccagacg gtccgtcacc
      541 ttctccgcca gctccagccg cgcccggtgg aggcgcgact tgacggtccc cgccggtacc
      601 tcgagatagg cggcggtctc ctctacgctc cacccctgga ggtcgcgcag cagcaccacc
      661 tcggccagct cctccggcat ggccgtcagc gcttcgtgga ccgcccgcag ctcctgccgc
      721 gcctgggcct gggcccgcgg gtcgtccgta gccggcatct gcagcaggat ctcctcggag
      781 acggcggtgg cgagccgctc ccggcgcacg cgccgccagt ggtcgatgca gagattgcgg
      841 gagaggcgca gggcccagcc gaggagcggg cagtcgccgc ggtagaggcg caggctctgg
      901 tagagccgca ggaagatctc ctgcgtcaga tcctcggcct cctccaccga cgcggagaac
      961 tgcagggcga gattgaacaa ccgtcccccg tggcgctcga tcagctcgtc ccaggcctgc
     1021 ggctgtccgg cggcggcccg gcgggcgagg agcaggtcag gggttgggag cggggcgtcc
     1081 atgagcgtcc tttccctcta tgacggaaag cggagggaaa aggttcacgg gaccgccgcg
     1141 cctagagctt ctccagaatc tcgaagagct cttcccggga gatccctgcc gtcttcaggt
     1201 tgttgcggat gatggagatg gggacctcgt cgtacgtcgg gatgaccacc ggccggagag
     1261 tgcccggccg gacatacgag cggtgagagc cctcctgccg ggccaaggtg aagcccaccg
     1321 ccaggaagac cttctcgagc acccgccaat ggagaggagt gaggcgcggc aagtcacacc
     1381 gcgagcgcga gatcgtgctt ctccacggat acgaagctgg ggctcaccca gcggtcgccg
     1441 tgaagctcgt agccgctctc ttccagaacc tcttccaggg tgccggcgtc ggtcgcggtg
     1501 aggaggaaaa ggcggaccgc ttcatcgacc gcctgccggg ccaattcggg agtcgcgccg
     1561 ctgctcatca cgtcgagagg catggcatgg gcaacgtact gcccgccctc tttccagatg
     1621 tggaccgtta cgtcgagatg catgatccta ttctaccccc agcaggaagc tcgccccctt
     1681 ccagatccac cagaggccga caaaccggaa gacccagagg agggcgcgca cgcccagcag
     1741 gaagaagttg tggaccggga acggcacctc cacgggggag cgctccggcg ccagcagccc
     1801 cagaagatag ccgaacgccg ccaggttcac cgtgatgccg atgagcagcc ggaaccaggc
     1861 ggtcaccgtg gccaggggga cgtcgcgccc cagggcggtg aggagcaggg cgcccaggag
     1921 cagcagcagg gggacgaaga tccccaggcg gagcggccag cgccaggcat cgaggatggc
     1981 gaagtacttc gccgcgccgc gccggtgccc ggggcagcag cgggcgcgaa gcgtctcctt
     2041 gccggcccgc acctccagga cctcggacgg cgcgtccggc gccagcacgc ggttgcacca
     2101 gacgcacttc acgggcagca cccgcgtcat gtacggccgc gccagccgcc ggtgccccac
     2161 catcagggcg acgccggcga ggagcgacag ccagaccggc tccgggtgcc ctacatagag
     2221 gtagaggacc agcgggatga agaggaagag gaggaccccg gtgatctgga ccgccagcgg
     2281 gcgggcgcgc ggcggcgtgg ccggtgaagg gtgggaggcc atttcaccgg cctcagatgt
     2341 ggcaggaggc caggccgcgc ttctccagat actgctggtg gtactcctcg gcgcggtaga
     2401 agatctgcgc gggctcgacc tgggtcacga tcggccggtt gaagcggccg ctcttgtcca
     2461 gcgtctcctt ggactcccgc gccgcccgct cctgctcggg ggtgtggaag aagatggccg
     2521 agcggtactg cg
//
LOCUS       DASPJI010000589         5027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_208118, whole genome shotgun sequence.
ACCESSION   DASPJI010000589 DASPJI010000000
VERSION     DASPJI010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208118"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..>5027
                     /locus_tag="VEL74_16495"
     CDS             15..>5027
                     /locus_tag="VEL74_16495"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64177.1"
                     /translation="MPHERALRIDAAVLEGPCAGDPPPLAGPGNLAYVLYTSGSTGRP
                     KGVAVEHRSPVELVHWAWDAFSGEELDGVLASTSICFDLSVFELFVPLSRGGTVLLAE
                     NALELPSLPAAGQVTLINTVPSAMSELAEGHLPPGPCTVNLAGEPLQPELVRRIYRHE
                     QVRRVLNLYGPSEDTTYSTWDPVEKGAERVTIGRPLHNTRAHLVDRLLQPVPVGVPGE
                     LCLEGAGLARGYLGRPDLTASRFVPAPLGGSGERLYRTGDLARRLLDGRLEFLGRLDH
                     QVKIRGFRIELGEVESVLAEHSEVREAVVVARDDLAGGRALVAYVVARPEGDLVLGEL
                     RSWLRSKLPASMVPAFLVLLQALPRTPNGKVDRRALPRPLQAAQPDTLAPRTPVEELV
                     AGIWADLLGLDRVGPDDNLFELGAHSLLAMRFASRLRQLFGCELPLRHFFQDPTVTCL
                     AARLEAARESGGGREVPSPLVRIAPELRERPLPLSFAQQRLWFIDQLEPGSPLYNMPV
                     ALRAEGPLDCGVLALTLGEIVRRHEGLRTVFAVLEGSPVQAIQAAAPFILPVVDLSGL
                     TESRREAVALSLAGEEAGRPFDLARGPLLRGLLLRLAGEDHVAALTLHHIASDGWSMG
                     ILVRELATLYAAFAQGRPSPLPELPVQYADFAVWQRSWLNGERLENEISFWRRQLAGL
                     PPWLELPTDRPRPATQSFRGATRLVRLPAGRIRQVQALSRDEGATLFMVLLAALQALL
                     ARYSRQQDLAVGSPIAGRNRAETEGVIGFFVNTLVLRGEVTGGTFRELLGRTRETALA
                     AYAHQDVPFEKLVEELAPERSLAHAPLFQMMLVLQNAPAEALELQSLRLRPAGVPGTT
                     AKFDLTLSLGEHNGELSGGITYATDLFDAVTIDRLTGHFERLLTGALESREQRLSEIS
                     LLTSAEHEQVVVEWNDTTHPFGEPSCVHHLIAARVAQAPEAEAVVCAGRSLSFGELGR
                     RAAGLARRLREQGVGPEVPVVLFLDRSVETIVALAGVLAAGGAYVPVDPTYPPEWVAW
                     VLADSRAPVVVTTSSLAGRLPAGTPTLLLEEVAESDATFDSAAGPDHPAYLIYTSGST
                     GRPKGVVVRHGSLMNLTRALREMVYRDDAGPLRVGVNASFAFDGSVKQIVQLAWGHCL
                     HILPQDVRLDPVAMVETVRRQRLDVLDCTPSLLRPLLQAGLGEGDAAPAMVLVGGEAV
                     DAELRDLALTRTRTRFWNVYGPTECTVDTVAAPFAAGTSSSRIGLPLSNVRVYVADSP
                     EDLAPLGVPGELQVGGAGLARGYLGRPDLTAERFIPDSFGGGAGERLYRTGDLVRWLP
                     DGSLDFLGRLDHQVKIRGFRIELGEIEAVLAALPAVRKALVVAREDTPGARRLVAYVV
                     GEVTADALRRALRERLPEHMVPAAFVMLAELPLTPNGKVDRKALPAPDGHAAEKSYQA
                     PRTPMEQILARIWSELLDLERVGVDESFFDLGGHSLLATRLVSQLRNTLRLEVPLRTL
                     FEAPTVAELARRVTALQSEQVAEAMPIRPVPRDGELPLSYAQQRLWFLDRMVPDNPFY
                     NVYHPVELSGTMDVEVLRRSFHEIARRHEVLRTTFDAVEGRPVQAIAPEVGLDLPLID
                     LQGLHGERRREELSRLSWEEGRRPFNLLRGPMLRLHGVRLEPEHHALLVTMHHIATDG
                     WSM"
BASE COUNT          696 a         1752 c         1751 g          828 t
ORIGIN      
        1 ggggagcgga agcgttgccc cacgagcgcg cgttgcggat cgacgccgcg gtgctggagg
       61 ggccatgcgc aggtgatcca ccacctctcg ccggtccggg caatctcgct tatgtcctct
      121 acacatcggg ctccaccggc cggcccaagg gggtggccgt cgaacaccgc agcccggtcg
      181 agctggtgca ctgggcctgg gacgctttct ccggcgagga gctggacggc gtgctggctt
      241 cgacctcgat ctgctttgat ctctccgttt tcgagctgtt cgttcccctc tcccgcggcg
      301 gcacggtgct cctggcggag aacgccttgg agctgccaag cctccccgcc gccgggcagg
      361 taacgctcat taacacggtc ccttcggcca tgtcagagct ggctgaaggc cacctgccgc
      421 cgggcccctg cacagtcaat ctggccggcg agccgcttca acccgaactg gtgcggcgga
      481 tctaccggca tgaacaggta cggcgggtgc tcaacctcta tggcccgtcg gaggacacga
      541 cctactcgac ctgggacccg gtggagaagg gcgcggagcg ggtcaccatc gggcgcccgc
      601 tgcacaacac gcgagcccat ctggtggacc ggctgctcca gccggtgccg gtgggagtgc
      661 ccggtgagct gtgtcttgaa ggggccggcc tcgcccgcgg ctatctagga cgaccggatc
      721 tgacggcctc ccggttcgtg cccgctcccc tgggcggatc gggcgaacgt ctctaccgca
      781 cgggcgatct ggcccggagg ctgctcgacg gccgcctcga gttcctcggc cgcctggacc
      841 accaggtgaa gatccgcggc ttccggatcg agctggggga ggtcgagtcg gtgctcgcgg
      901 agcattccga ggtccgcgag gcagtcgtgg ttgcgcggga cgacctcgcc ggcggccggg
      961 cgctggtcgc ctacgtcgtg gcgcggcccg agggcgatct ggtcctgggc gagttgcgga
     1021 gctggctgcg gtcgaagctc cccgcctcca tggtgccggc gttcctcgtc ctgctccagg
     1081 ccttgccgcg gacacccaac ggcaaggtcg accgccgcgc cttgccacgt ccgctccagg
     1141 ccgcgcaacc ggacacgttg gcgccacgga cgccggtgga ggaactggtg gcggggatct
     1201 gggcggatct cctgggtctc gaccgtgtgg ggccggacga caacctcttc gagctcgggg
     1261 cccattcgtt gctggccatg cggttcgctt cccggctgcg gcagcttttc ggctgcgagc
     1321 taccgctgcg gcacttcttc caagacccta ccgtcacctg ccttgccgct cgactggagg
     1381 cggcgagaga gtcaggcggc ggcagggagg tgccttcacc tctggtacgc atcgctccag
     1441 agttacgaga gaggcctctg cccctctcgt tcgcacagca gcgcctctgg tttatcgatc
     1501 agctcgagcc gggtagtccg ctgtacaaca tgcctgtggc tctacgtgcc gaagggccgc
     1561 tcgactgtgg tgtgctggcg ctcaccctcg gggagatcgt gcgtcgccac gaaggcttgc
     1621 gcacggtctt cgccgtgctg gaaggttcgc cggtgcaggc gatccaagcc gcagccccct
     1681 tcattctgcc tgtagtggac ctctcggggc tgacggagag ccggcgcgag gctgtggccc
     1741 tgagcctggc cggcgaagag gccggccgcc cgttcgacct cgcccgtggt ccgctgctgc
     1801 gcggcctgct gctgcggctg gccggagagg accacgtcgc ggccctgacc ctgcatcaca
     1861 tcgccagcga cggctggtcc atgggcatcc tggtccgcga gttggcgacg ctctacgcag
     1921 cgttcgccca gggcaggcct tcgccattgc cggagctgcc ggtgcaatac gcggacttcg
     1981 ccgtctggca gcgctcctgg ctcaacggcg agaggctgga gaacgagatc tctttctggc
     2041 gccggcagct cgccggtctg ccgccatggc tggagctccc caccgaccgg cctcgtcccg
     2101 cgacacagag ctttcgtggc gctacccggc tcgtgcggct gccggccggg cgcatccgac
     2161 aggtgcaggc cctgagccgg gacgaaggag cgaccctctt catggtgctg ctggccgcat
     2221 tacaggctct cctggcacgg tacagccggc agcaggatct cgcggtaggc agcccgatcg
     2281 ccggacgcaa ccgcgctgaa acggaagggg tgatcgggtt ctttgtcaac acactggtgc
     2341 tgcgcggcga ggtgaccggc ggcacgttcc gtgagctgct cggccggacc cgcgaaaccg
     2401 cgctggccgc ctacgcccac caggacgtgc cgttcgagaa gctggtcgag gagctcgcgc
     2461 cggagagaag cctggcccat gcccccctgt tccagatgat gctggtcttg cagaacgctc
     2521 ccgccgaggc gctggagctc cagagcctgc ggttgcggcc ggcgggcgtt cccgggacga
     2581 cggcgaagtt cgatctcacc ctatctctgg gggagcacaa cggcgagttg agcggtggga
     2641 ttacctacgc cacggatctg ttcgacgccg tcaccatcga ccgtctgacc ggacacttcg
     2701 agaggctgct caccggggct ctcgagagcc gcgagcagcg gctctcggag atctccctgc
     2761 tgacttcggc ggagcacgag caggtggtgg tggagtggaa cgacaccacg cacccgttcg
     2821 gcgagccctc gtgtgtgcac cacctgatcg ccgcccgggt cgcccaggct cccgaagccg
     2881 aggcggtggt ctgcgcgggc cgcagcctga gcttcggcga gctcggccgg cgggccgccg
     2941 gcctcgcccg ccgcctcaga gagcagggcg tcgggcccga ggtcccggta gtcctcttcc
     3001 tcgatcgctc ggtggaaacc atcgtggctc tcgccggcgt gctcgcggcg ggaggcgcct
     3061 atgtacccgt cgatcccacc tacccgccgg aatgggtcgc ctgggtgctc gcggactctc
     3121 gggcgcccgt ggtcgtcacc acctccagcc tcgccggaag gttgcccgcg ggcacgccca
     3181 ccttgctcct cgaagaggtg gcggagagcg acgcgacctt cgacagcgcc gcgggtccgg
     3241 accatccggc ctacctgatc tacacctccg gctcgaccgg ccggcccaag ggagtcgtgg
     3301 tccgccacgg ctcgctgatg aatctgacgc gggcgctgcg cgagatggtc tatcgagacg
     3361 acgcaggccc gctgcgggtg ggcgtcaatg cctcgttcgc cttcgacggc tcggtcaagc
     3421 agatcgtgca gctcgcctgg ggccattgcc tccacatctt gccccaggat gtgcgcctcg
     3481 accccgtagc gatggtggag accgtccgcc ggcaacggct cgacgtgctc gactgcacgc
     3541 cctcactgct ccgtccactc ctccaagccg gtctgggcga gggtgacgcc gcgcccgcca
     3601 tggtcctggt gggcggcgaa gccgtggacg ccgagttgcg cgacctcgcc ctcacccgca
     3661 cgcggacccg cttctggaat gtgtacggac ccaccgagtg cacggtggac accgtggccg
     3721 cgcccttcgc ggcgggtacg tcgtcgtccc gcatcggtct gccgctttcg aacgttcggg
     3781 tctacgtcgc cgacagcccg gaggacctcg cccccctggg ggtgcctggc gagctccagg
     3841 tgggcggggc ggggctggcc cgcggctacc tggggcggcc cgatctgaca gctgaacggt
     3901 tcatccccga ctcattcggc ggcggcgccg gcgagcgtct ctaccgcacg ggcgacctcg
     3961 tgcgctggct gcccgacggc tccctggact tcctcggccg cctggaccat caggtcaaga
     4021 tccgcggctt ccgcatcgag ctgggagaga tcgaagccgt gctggcggcg ctgcccgcgg
     4081 tgcggaaggc tctggtggtg gcgcgggaag acacgcccgg cgcccgccgg ctcgtggcct
     4141 atgtggtcgg tgaggtcacg gccgacgctc tacgccgggc cctgcgggag cggctgccgg
     4201 agcacatggt gcccgccgcc ttcgtgatgc tcgcggagct gcccctcacc cccaacggca
     4261 aggtggatcg gaaagctttg ccggctcccg acggccacgc tgcggaaaag agctatcagg
     4321 cgccacgcac gcccatggaa cagatcctcg cccgaatctg gagcgagctc ctcgatctcg
     4381 aaagagtcgg ggtggacgag agcttcttcg acctgggcgg ccactcgctg ctggcgacgc
     4441 gcctggtatc acagctacgg aacaccctgc gactggaagt gccgctgcgg accctgttcg
     4501 aggcgccgac cgtggcggag ctggctcggc gggtgacagc cctccagagc gagcaagtgg
     4561 ccgaggcgat gccgatccgt ccggtgccgc gcgacggcga gctgccgttg tcctacgcgc
     4621 agcagcggct ctggttcctc gaccgcatgg ttccggacaa cccgttctac aacgtgtacc
     4681 acccggtcga gttgtccggg accatggacg tcgaagtact gcggcgcagc ttccatgaga
     4741 tcgcgcggcg tcatgaggtg ctgcggacga ccttcgacgc ggtggaaggt cggccggtcc
     4801 aggccattgc ccccgaggtg gggctggacc tcccgctgat cgacctccag gggctacacg
     4861 gcgaacggcg gcgggaggag ctgtcgcggc tctcctggga ggagggacgc cggccgttca
     4921 acctgctccg ggggccgatg ctgcggctgc atggggtgcg cctggagccc gagcaccacg
     4981 ccctcctggt caccatgcac cacatcgcca cggacggctg gtccatg
//
LOCUS       DASPJI010000590         5442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_94919, whole genome shotgun sequence.
ACCESSION   DASPJI010000590 DASPJI010000000
VERSION     DASPJI010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94919"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VEL74_16500"
     CDS             complement(<1..191)
                     /locus_tag="VEL74_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64178.1"
                     /translation="MPTRQTDSPLSPCVLVVKTPVASRKRVVDRLAQTGPRLAGEVTD
                     AASEQELTLATLHEALAGL"
     gene            complement(217..588)
                     /locus_tag="VEL74_16505"
     CDS             complement(217..588)
                     /locus_tag="VEL74_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64179.1"
                     /translation="MDYLVLLFLAALAVFVVSLCLRSRHPFILALLFVVLVPLLAIAM
                     LFFLPGQVDGLREMWYANQGGGDPFGSGMMVAMFMTAGALGLAGALCIVIPLALIWRK
                     GARRTDTRRSSSDWDRLSNAP"
     gene            723..1196
                     /locus_tag="VEL74_16510"
     CDS             723..1196
                     /locus_tag="VEL74_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64180.1"
                     /translation="MKPILRFTLPLLFVSLATPALAGECKKIRAEIVDVQVTEGCTSP
                     NRFCAAGTVKGNHGLNGTTYFRMDGAVRGPATAPGSIATSGILTYTTDRGTLTARESG
                     LSGLIESPEEGFFTSFQEVLEGTGDYAGATGHLWVLGHRIEDLFVSEVRGEICQP"
     gene            1272..2087
                     /locus_tag="VEL74_16515"
     CDS             1272..2087
                     /locus_tag="VEL74_16515"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYG64181.1"
                     /translation="MTIDRSTSRPPRTAFGEVLRTYRRGTGQPQLEAALSAGISQRHL
                     SFLETGRAQPSRGVVLALGGTLGLSLPQQNELLLAAGYAPIFKPERDPKELELIERAL
                     LAFLDVHEPFPALLLDEGVWIKKGNRGAARLWSAVRGVPIPEVPQDNIFEVMLRRGPG
                     REKLENWSEAAACLLRRYMAEQARSGRGLSEAAAALARNREVKRLLRQDPEAPPPPVV
                     LLKYRFDDARLNLFIVIASLQAPLDTRLEDLRVELFIPADEATAEWFRREAQG"
     gene            2124..2408
                     /locus_tag="VEL74_16520"
     CDS             2124..2408
                     /locus_tag="VEL74_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64182.1"
                     /translation="MPRATVAVPIPEDLARQLPADPAERDQVLVLGIREWKVRQALEA
                     YKRGEGSLAFAARQAGVSFREIIPLAYAYGLTPKVDSTDLEASEDWTSHL"
     gene            2405..2926
                     /locus_tag="VEL74_16525"
     CDS             2405..2926
                     /locus_tag="VEL74_16525"
                     /inference="COORDINATES: protein motif:HMM:NF023276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HYG64183.1"
                     /translation="MIVVADAGPLLALAKTDGLGVLFRLYPKIYSPPAVYEEVVTAGH
                     RLSAPDAAIVEECFRSGHLELSEPTRASLPVSRELGRGEEEGIRLALQKGADWLLMDD
                     LDARRAAEACFRAAGAGTRVQGTLGVIVSAYQQNHLGRLEAIEIVERLNQRLDVWISP
                     ALCRRVIDLLKEG"
     gene            complement(3157..3741)
                     /locus_tag="VEL74_16530"
     CDS             complement(3157..3741)
                     /locus_tag="VEL74_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64184.1"
                     /translation="MFEYLMSNKEWIFSGAGVVALSTISAIIWRLAKKKAGPPTPQVI
                     VVQASQTHGSSSTTAPALKSSETPVATPAPITRITPISISEIRETVSDAPPLQKNQVA
                     KNYEGLHVEWDSKLSNAETKGGDNVLLLLVIGGPIGEYIYCEVKLSDYKELGILKKGA
                     PIRVTGRIKKVKDAYLELDEVQLFFTPPSEGASS"
     gene            complement(3956..>5442)
                     /locus_tag="VEL74_16535"
     CDS             complement(3956..>5442)
                     /locus_tag="VEL74_16535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG64185.1"
                     /translation="SQRLLGLVDQLLDLARHDAGQLRLKVRRGDLAALLRQRVESFLP
                     LAERCRVELSLRLQEGRAELYFDAAELEKVFDNLLSNALKFTPPGGRVDVALTAPPGS
                     GRVEVTVEDSGPGIPADQVDRVFDRFYQVESADRRPSPGVGLGLALARHLVELHGGSI
                     AAASREGKGARFTVTLRRGRDHFPAELLDETAPPAAAPAEAPDQAPVRSRALPTPLPP
                     LPEPGRIQPAPDDERMTVLVVDDNADIRAYIRRHLEPGYRVVEAADGAEALAQARRSV
                     PDLVVSDIMMPGLDGHALFRALRLEPELELVPVILLTAKASPESRLQGLREGADDYLL
                     KPFDPWELKARVDNLIASRRRLAERLTGVPQPLRAREIEATAAATPAATPADEAFVAR
                     AQAVIEERLGDPELTVEALAAALRCDRSYLLRKLRALTGESPSDLIRSLRLQRAEQLL
                     KTGAGTVGEIAYAVGFKSVAHFSNAFHQRYGERPSAFALRHRSR"
BASE COUNT          871 a         1768 c         1896 g          907 t
ORIGIN      
        1 tccaggccgg cgagggcctc gtgcagggtg gcgagagtca gctcctgctc gctcgccgcg
       61 tccgtcacct cgccggcgag ccgcgggccg gtctgcgcca ggcggtccac cacccgtttg
      121 cgggaggcca ccggcgtctt caccaccagg acacaggggg agagagggga atcggtctgt
      181 cgggtcggca cgcgcgaaga ctatcgcact ggcgcgtcag ggggcgttgg agagacgatc
      241 ccagtcggag gaggagcgcc gcgtgtcggt cctccgggcg cctttcctcc agatcagggc
      301 cagggggatg acgatgcaaa gcgcgccggc cagcccgagg gcgccggcag tcatgaacat
      361 ggccaccatc atgcccgagc cgaagggatc tccccctccc tgattcgcgt accacatctc
      421 tctcagccca tcgacctggc ctgggaggaa gaacagcatg gcgatggcca acagggggac
      481 cagcacgacg aacagcagcg ccaggatgaa ggggtgccgc gagcggaggc agaggctcac
      541 gacgaagacg gccagcgccg ccaggaacag taggaccaga tagtccattt cgaagtctcc
      601 ctggtccgtc agtttactcc catacgactt gacctcgcac gtaattgatc cggcctccct
      661 cccggtgtga actgtcgatg cggcgaatga ctcgccgctc gaccgagatg gaggctttgc
      721 cgatgaaacc gatccttcgt ttcaccctgc cgctgctgtt cgtctccctg gccacccccg
      781 cccttgccgg cgagtgcaag aagatccgcg ccgagatcgt cgatgtccag gtgaccgagg
      841 ggtgcaccag ccccaaccgg ttctgcgccg ccgggacggt caagggcaac cacgggctca
      901 acggcaccac ctatttccgg atggacggtg cggtgcgcgg cccggcgacg gcgccggggt
      961 ccatcgccac cagcggcatc ctgacctaca ccaccgatcg cggcaccctg acggcgcgcg
     1021 agagcgggct ctccggattg atcgagtcgc cggaggaggg tttcttcacc tcgtttcagg
     1081 aggtcctcga agggaccggg gactacgccg gcgccaccgg ccacctctgg gtcctgggcc
     1141 accggatcga ggacctcttc gtgagcgagg tcagagggga gatttgccag ccatagaccc
     1201 ttgcgcataa tgaggctttc atgaagcgga tgggcgcggc gcacgccgcg cccatccgcc
     1261 cgggagctct catgacgatc gaccgaagca cctcgcggcc cccacgcaca gcttttggag
     1321 aggtcttgcg cacgtatcgc cgcggcacgg gccagccgca gctcgaggcc gccctctccg
     1381 ccggcatctc gcagcgtcac ctcagcttcc tcgagaccgg ccgcgcgcag ccgagccgcg
     1441 gcgtcgtgct ggcgctcggc ggcacgctgg ggctgtcgct gccgcagcag aacgagctgc
     1501 tcctcgcggc tggctacgcg ccgatcttca agcccgagcg cgacccgaag gagctggagc
     1561 tgatcgagcg ggcgctcctc gccttcctgg acgtgcacga gccctttccg gcgctgctgc
     1621 tcgacgaggg ggtgtggatc aagaaaggga accgcggcgc ggcgcgcctc tggtcggcgg
     1681 tgcggggcgt tcccattccg gaggtgccgc aggacaacat cttcgaggtg atgctgcggc
     1741 ggggtcctgg gcgcgagaag ctggagaact ggagcgaggc ggccgcctgt ctcctgcgcc
     1801 gctacatggc ggagcaggcg cggtccggcc gcgggctctc cgaggccgcc gcggcgctgg
     1861 cccgcaatcg cgaggtcaag cgcctgctcc ggcaggaccc cgaagcgccg ccgccgccgg
     1921 tcgtgctgct caagtatcgc ttcgacgacg cgcgcctgaa tctgttcatc gtcatcgcca
     1981 gcctgcaggc gccgctcgat acccgtctgg aagacctgcg cgtcgagctg ttcatccccg
     2041 ccgacgaagc gacggcggag tggttccggc gggaggcgca gggctgagcc gtgccagcca
     2101 catccacgtg ttaccatcgc tccatgccga gagccaccgt cgccgtaccc attcccgaag
     2161 atttggcgcg ccagctgcca gcggatcctg ccgagcgcga ccaggtactg gtcctcggaa
     2221 ttcgggaatg gaaggtgcgc caggcgctgg aggcttacaa gcgcggcgag ggctcgctgg
     2281 cgtttgccgc ccgccaggcc ggcgtctctt tccgtgagat cattcccttg gcctacgcct
     2341 atgggctgac gccgaaggtc gattccacgg atctcgaagc ctcagaggat tggacctccc
     2401 acctttgatc gtcgtagcgg acgcgggtcc gctgctggcg ctggccaaga ccgatgggct
     2461 tggggttttg ttccggctct acccgaaaat ctattctcct cccgcagtct acgaagaagt
     2521 cgtgaccgca gggcaccgcc tgtcggcccc ggatgccgcc attgtcgaag agtgcttccg
     2581 ttccgggcat ctcgaattgt cggagcctac gcgagcctcg ttgcccgtgt cgcgcgaatt
     2641 gggaagggga gaggaggagg gcatccgact ggcgttgcag aaaggagccg actggctcct
     2701 gatggatgac ctcgatgccc gacgggcggc agaggcctgc ttccgcgccg caggagccgg
     2761 aacccgagtt caggggacgc tcggggtcat cgtctcggcc taccagcaga accatctcgg
     2821 acgcctggag gctatcgaga tcgtcgagag actgaatcag cgcctcgatg tctggatcag
     2881 ccctgccctg tgccggcggg tgatcgatct cttgaaagag ggctgagctc tctaccggat
     2941 catcgccgcc ggatcaccgc gagcggtggc ggagggcgaa ggcggagggg cgctcgccct
     3001 agcgctggtg gaaggcgttc aagaagtgcg cgacgctctt gaagccgacg gcgtaggcga
     3061 tctcgcccac cgtgccggcg ccggtcttga ggagctgctc ggcgcgctgc aggcgtagcg
     3121 accggatcag atcggagggg ggcgcagccc tggagcttag ctggatgcgc cctcagatgg
     3181 tggagtgaag aacagctgaa cctcatccaa ctcgagatag gcgtccttaa cctttttgat
     3241 tctgccggtg acccgaatgg gggcgccctt tttcaagatg ccgagttcct tgtagtcaga
     3301 gagttttact tcgcagtaga tgtactcccc aatcggcccc ccaatgacga gaagcagcaa
     3361 gacgttatcg cctcccttcg tctctgcgtt ggagagtttc gagtcccatt cgacatggag
     3421 accctcgtag ttcttcgcca cttgattctt ttgcaaggga ggggcatcac tcactgtttc
     3481 gcggatttcc gaaatcgaga ttggagtgat ccgcgttatg ggtgctggag tcgctactgg
     3541 cgtttcgctt gacttcaatg ctggcgcggt ggtcgagcta ctgccatgag tctgcgaagc
     3601 ttggacgact atcacttgcg gagtaggggg accggccttc ttcttggcaa gtctccagat
     3661 aattgccgat atcgtggaca gagcgactac gccggctccg gagaatatcc actccttgtt
     3721 gctcattaga tactcgaaca cgagactatc ctccgtctgc accgatctta gggctcgaca
     3781 tgctcggttg tgtcggtaag gcttggcgat gcttccctga gcatgcctgt gctgctggtg
     3841 gcctctcaaa cgtcagtacg tcggcagttt aaggcgcttt taagacgagg gtcaaggcgg
     3901 caatggctgt cgtcctgact gcttgttccg aagaggaatg gggggagacc ctttgtcacc
     3961 gcgagcggtg gcggagggcg aaggccgagg ggcgctcgcc gtagcgctgg tggaatgcgt
     4021 tggagaagtg ggcgacgctc ttgaaaccga cggcgtaggc gatctcgccc accgtgccgg
     4081 cgcccgtctt gaggagctgc tcggcgcgct gaaggcgtag cgagcggatc aggtcggagg
     4141 gggactcgcc ggtcagggcg cgcagcttgc gcaggagata ggagcggtcg cagcgcagcg
     4201 ccgcggcgag cgcctcgacg gtcagctcgg gatcgcccag gcgctcctcg atcaccgcct
     4261 gcgcccgcgc cacgaaggct tcgtccgccg gggtagccgc cggggtcgcc gcggccgtgg
     4321 cctcgatctc cctggcacgg agcggctgcg gcacgcctgt gagccgctcc gccagccgcc
     4381 ggcgggaggc gatgaggttg tccacccgcg ccttgagctc ccacgggtcg aacggtttga
     4441 ggaggtagtc gtcggcccct tcgcgcagcc cctggagccg gctctcgggc gaggccttgg
     4501 cggtgaggag gatgaccggc acgagctcga gctcgggctc caggcgcagg gcgcggaaga
     4561 gggcatggcc gtcgagcccc ggcatcatga tgtcgctgac cacgaggtcg gggacggagc
     4621 ggcgcgcctg cgcgagcgct tcggcgccgt cggcggcctc gaccacccga tagccgggct
     4681 ccaggtggcg ccggatgtag gcgcggatgt cggcgttgtc gtccaccacc agcacggtca
     4741 tccgctcatc gtctggcgcg ggctggatgc ggccgggctc gggcaggggc ggcagggggg
     4801 taggaagggc gcgggacctg acgggtgcct ggtccggcgc ctccgcgggc gccgcggcgg
     4861 gcggggcggt ctcgtccagg agctcggcgg ggaagtggtc ccggcctcgc cgcagggtga
     4921 cggtgaagcg ggcgcccttc ccttccctgc tggccgccgc gatcgagccg ccgtgcagct
     4981 ccaccagatg gcgggcgagg gccaggccga ggcccacccc cggcgagggg cgccggtccg
     5041 cggactccac ctgatagaaa cggtcgaaga cacggtccac ctggtccgcc gggatgccgg
     5101 ggccgctgtc ctcgacggtc acctccaccc tgcccgagcc ggggggcgcc gtcagggcca
     5161 cgtccacccg gccgccgggc ggcgtgaact tgagggcatt gctcagcagg ttgtcgaaca
     5221 ccttctccag ctccgccgcg tcgaagtaga gctcggccct cccctcctgc agccgcagcg
     5281 aaagctcgac gcggcaccgc tccgccaggg ggaggaacga ctcgacgcgc tggcgcagca
     5341 gcgccgcgag gtcgccgcgg cgcaccttga gccggagctg tccggcgtca tgtctggcga
     5401 ggtcgagcag ttggtccacc agaccgagca ggcgctggga gt
//
LOCUS       DASPJI010000591         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_551283, whole genome shotgun sequence.
ACCESSION   DASPJI010000591 DASPJI010000000
VERSION     DASPJI010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551283"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2611
                     /locus_tag="VEL74_16540"
     CDS             <1..>2611
                     /locus_tag="VEL74_16540"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG64186.1"
                     /translation="AEPETGLECPPGRVGEIWIAGPSVARGYWRNAEATARDFGAVLT
                     GLPGEVDGPFLRTGDLGFVADGELYVTGRLKDLVIIRGRNHYPQDLELTAESSHPDLR
                     AGSGAAFAVEVEGEERLILVQEVERRLRGDVSEVAEAIRQAVAAEHEVQVWEVVLVRP
                     GGVPKTSSGKVRRSACRAAYLEGELPVVGRSALSSRQLPAERVVVPPPLGGWTVGSGG
                     GQEGGFLPWLRERAARLLGMAPASLELDRPLTALGLDSLSAVELKGQAEVALGVELPL
                     ADLLQGASLGELAERLEGGASLGEVPPLRALPREGDQPVSFGQRSLWFLERLAPEAGA
                     YNIAVAARVVSGELDVEALGRALARLVARHEGLRTVFPAPDGKPVQRVLPKVPVLVEE
                     GSRAEAWRPFDLEHGPLLRVVLDGPVVLFVVHHIVSDFWSLAVAARELSALYREETGG
                     PAANLPPLPLSYVDFTHWQAATLAGPRGEALWEQWRERLAGLPDLELPTDRPRPPVQT
                     WSGLARGVEIPATEADAVRSLAARHGATMFTALLAAFQTQLGRYAGQDDFAVGSPTVG
                     RGAPELDGLIGYFVNPVPLRADLAGNPSFGILLDRTRRTAVEGLAHSGFPFALLAERL
                     RPARDPARLPVFQALLVLEQRRAGDPPGMPAFALSEDGARIPFADLELESVRLEERRA
                     QLELSLSAAELPSGGLGISLEVNADLFDGATAERMLGHLRTLLAAAAADLDTPLDLLQ
                     LLTQLERAQLSRDWSTAPVVAVPDLCLHELVAEQALRRPEAEAVVAGDESLTYRELVE
                     RARALAERLRGLGVEPEKPVALCVERSAEMIVGVLGILEAGGVYLPLDPDHSAARLGF
                     VLEDAGAAAVVTDA"
BASE COUNT          305 a          825 c         1063 g          418 t
ORIGIN      
        1 ggcggagccg gagacggggc tcgaatgccc gccgggccgg gtgggggaga tctggatcgc
       61 cggtccgagc gtcgcccgcg gctattggcg caacgccgag gccaccgcgc gcgacttcgg
      121 ggcggtgttg accgggcttc ctggcgaagt ggacgggccg ttcctgcgca ccggcgacct
      181 gggtttcgtg gcggacggcg agctgtacgt caccggccgg ctcaaggacc tggtgatcat
      241 ccgcgggcgc aatcactacc cgcaggacct ggagctgacg gcggagtcga gccatccgga
      301 cctgcgggcc gggtcggggg cggcgttcgc ggtggaggtg gagggggagg agcggctgat
      361 cctggtgcag gaggtggagc gccgcctgcg gggtgacgtc tccgaggtgg ccgaggcaat
      421 ccgccaggcg gtggcggcgg agcacgaggt gcaggtctgg gaggtggtgc tggtgcgtcc
      481 gggtggtgtg cccaagacct ccagcggcaa ggtgcggagg agcgcctgcc gggcggctta
      541 tctggaaggt gagctgccgg tggtggggcg gagcgcgttg agctcccgac agcttccggc
      601 ggagagggtg gtggtgcctc cccccctggg tgggtggacg gtagggagcg ggggaggtca
      661 ggaggggggt tttctccctt ggctgcggga gagagcggcg cggctgctgg ggatggcgcc
      721 ggcgtctcta gaactggacc ggccactcac cgccctgggt ctcgactccc tctcggcggt
      781 ggagctcaaa gggcaggccg aggtggccct gggtgtcgag ctgcccctgg ccgacctgct
      841 ccagggggcg agtctggggg agctggcgga gcgactggag ggtggcgcca gcctcggcga
      901 ggtgccgccc ttgcgggctc tgccacgcga aggggaccag ccggtctcgt tcggtcagcg
      961 ctcgctctgg ttcctggagc ggctggcgcc ggaggccggg gcgtacaaca tcgccgtggc
     1021 ggcgcgggtc gtctccggag agttggatgt agaggctttg ggacgcgccc tggcgcggct
     1081 ggtggcccgg cacgaggggc tgcggacagt cttcccggct ccggatggca agccggtgca
     1141 gcgggtgctg ccgaaggttc ctgttctcgt ggaggagggt tcgagagctg aagcctggcg
     1201 tcccttcgac ctcgagcacg ggccgctgct gcgggtagtc ctcgacggtc ccgtggtgct
     1261 gttcgtcgtg catcacatcg tctctgactt ctggtccctg gcggtggcgg cccgcgagct
     1321 gtcggcgctc taccgggagg agaccggcgg gccggcggcg aacctccctc ctctgccgct
     1381 gtcctacgtg gatttcaccc attggcaggc ggcgaccctc gccgggccgc gaggggaagc
     1441 gctgtgggag cagtggcgag agcgcttggc gggcctgccc gatctggagc tgccgacgga
     1501 ccggccgcgt ccgccggtcc aaacctggag tggcctggcc cgaggagtgg agatccccgc
     1561 taccgaggcg gacgcggtcc gctcgctggc ggcgcggcac ggggcgacga tgttcacagc
     1621 gctcctcgcg gccttccaga cccaactcgg ccgctatgcc ggccaggacg acttcgccgt
     1681 gggctcgccg accgtgggcc gcggcgctcc ggagctggac ggcctgatcg gctacttcgt
     1741 gaatcccgtt cccctgcggg cggacctcgc ggggaacccc tccttcggca tcctcctgga
     1801 ccggacgcgg cggaccgccg tcgaagggct cgcccactcc ggcttcccct tcgcactgct
     1861 ggcggagcgg ctccgcccgg cgcgcgatcc ggcccggctg ccggtcttcc aggcgctgct
     1921 cgtgctggag cagcggcgcg ccggcgatcc ccccggcatg cccgccttcg ccctaagcga
     1981 ggacggcgcc cgtatcccct tcgcggacct ggagcttgag tcggtccgcc tggaggagcg
     2041 gcgggcgcag ctcgagctgt cgctgagcgc cgcggagctg ccctctggcg ggttggggat
     2101 ctccctggag gtcaatgccg atctgttcga cggtgctacg gccgagcgca tgctgggcca
     2161 tctgcgaacc ctgctggccg cggctgccgc cgatctggat acgccgctcg atctcctgca
     2221 gttgctgacc cagctggagc gtgcgcagct ctcgcgcgac tggagcacgg cgccggtggt
     2281 ggcggttccg gatctctgtc tgcacgagtt ggtggcggag caggccctgc ggaggcctga
     2341 ggccgaggcc gtggtggctg gcgacgagag cctgacgtat cgcgagctgg tggagcgggc
     2401 gcgggcgctg gcggagcgat tgcgggggct tggtgtcgag ccagaaaagc ccgtggccct
     2461 gtgcgtcgag cggtcggcgg agatgatcgt gggggtgctc ggcatcctgg aggccggggg
     2521 ggtgtatctg cccctggacc cggaccactc ggcggcgcgg ctgggattcg tgctggagga
     2581 cgctggagcg gcggcggtgg tgacggacgc g
//
LOCUS       DASPJI010000592         6041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_784951, whole genome shotgun sequence.
ACCESSION   DASPJI010000592 DASPJI010000000
VERSION     DASPJI010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784951"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>6041)
                     /locus_tag="VEL74_16545"
     CDS             complement(<1..>6041)
                     /locus_tag="VEL74_16545"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64187.1"
                     /translation="LAGIFAAVLGAERVGADESFFELGGHSLLATRVMSRVREAFGIE
                     LPLRALFEAPTVSGLAAVVSASGEAPFQALPPLEPLAPAARELPLPLSFAQERLWFLD
                     QLEPGSAAYNIPLALRLEAGLETRLLESALGEVIRRHEALRTTFPMESGRPVQRIGPS
                     AAATGWGLVRVDLSALPAAPRDAEASRLAGHEADRPFDLSAGPLLRTTLLQLGEPGQW
                     LLFTVHHIVADGWSMDLLAEEVTALVQAFARGEASPLPELPVQYADFAVWQRRTLTED
                     RLAAHLSYWRGRLAGAPPLLELPTDRPRPAVQSARGGVRSLPLPEEVWRSLARLALER
                     GVTPFMALLAAFQMLLARYSGQTDVSAGTPVAGRTHLLAERLIGLFVNTLVLRADLSG
                     DPSFAALLGRTREVTLGAFSHQDLPFERLVEELQPQRSLSHTPLFQVMFTFQSTPSGG
                     LEDGRPLPDEAATARFDLSLTAAGGEQGGHLSLVYRAELFDAGTAERLLHHLSTLVAA
                     AVAEPERRLSDLPLLTEAERHELAREWNDTASISGTGGMGVPALCLHQLFDLQSSRTP
                     ESPAVESGDERLTFQDLRQRANALAHRLRRLGVGPESRVGLCMDRSAAMVVGLLGILK
                     AGGAYVPLDPSYPAERLAFLLEDAGIAALVTRSGPAAALPPSSLPLVLLDGALDEPVT
                     DPEPLSLPEHLAYVIYTSGSTGVPKGVMVRHGSVVNLLASLGATVYAGLDAPLRVAVN
                     APLAFDASVKQWIQVLAGHTLVLVDEESRLDPGRLLALLESRRVDVLDCTPGQLRLLL
                     EAGLTETAAAPRRVLVGGEDIPEDLWTSLASAAPGSVYNVYGPTECTVDATVMPVATV
                     RPALGRPLPNVRVHLLGPGSEPVPTGVPGELCIAGAGLARGYLGRPDLTAERFVPDPS
                     GWPGDRLYRTGDLARHRPDGRIEYLGRIDHQVKVRGFRIELGEIEAALQAHPAVGAAA
                     VLAREDTPGDRRLVAYAATAEPRPGAAELRRFLAERLPEHMVPAAFVLLDALPLTPNG
                     KVDRRALPAPVTEAAPAAAPRTPVEELLAGIFAAVLGAERVGADESFFELGGHSLLAT
                     RVMSRVREAFGVELPLRMLFEAPTVAGLATAVEAATAAGRFAAAPPLVPAEQTGPLPL
                     SFAQERLWFLDRLQPGSAVYNMPLSLWLADGVDAAVLARALGEVVRRHEALRTTFHLT
                     DGGPVQRVSAAGSFPLPLVDLAPLPAGRRKAETLRLAGEEATRPFDLAAGPLLRATLV
                     GADGPARWLLLTLHHIVSDAWSMDLLVEEVTALYAAFGAGRPSPLPELPVQYADFAVW
                     QRAWLAGEELERQLAFWRGQLAGAPASLELPADRPRPVQPSHRGARHIRRLSAPLEAG
                     LRRLGREQGTTLFMSLLAASAALLGRWAGQDDLVLGTPIAGRNRLEVERLIGFFVNTL
                     PLRVGLASGVCFRHLLRQVRETALAAYAHQDLPFEKLVEELRVARNLSHAPLFQVMLS
                     LPATGGPRGSGTEAPGLAVGTGTAKFDLTLEVREERDGLELGAEYGTDLFDGVTIDRL
                     LAHLERLLEDVARDPGLPPAEVPLLAGAERHALLVEWNDTGRERGEGLCLHRLFERQA
                     ALTPGAEALVTAAERLSYGELNRRANRLAHHLRRLGVGPEDRVGVRLERSAAMVVALL
                     GILKAGGAYVPLDPAYPAARLELMAEDADLTALVTRELLAAVPAAESELDPEPLVLPG
                     NLAYLIYTSGSTGRPKAVAIEHRSAVALVEWAGGVFTAEELSGVLAATSISFDLSVFE
                     LFVPLAYGGRVVLAANALELPELAARDEVRLINTVPSTMAELAAGPLPASVRTVNLAG
                     EALKVRLVERIYEHPQVERVLNLYGPSEDTTYSTFIVAVRGGRVTIGRPLTGTRVHLL
                     ERGGVEPVPLGAAGELCLAGDGLARGYLRRPGLTAERFVPDPFAGQPGERLYRTGDLA
                     RWLPDGHLELLGRIDHQVKVRGFRVEPGEIEAALQSHPDARTAVVLARAAAAGGL"
BASE COUNT          869 a         2231 c         2266 g          675 t
ORIGIN      
        1 gcaggccgcc cgccgcggcc gcgcgggcga ggaccaccgc cgtccgcgcg tcgggatggg
       61 attggagcgc ggcctcgatc tcccccggct cgacgcggaa gccccggacc ttcacctggt
      121 gatcgatgcg gcccagcagc tccaggtgtc cgtccggcag ccagcgcgcc aggtcgcccg
      181 tgcggtacag ccgctccccc ggctggccgg cgaaaggatc ggggacgaag cgctccgccg
      241 tcagcccggg acgccgcagg tacccccggg ccagcccgtc cccggccagg cagagctcgc
      301 cggcggctcc caggggcacc ggctccacgc cgccgcgctc cagcaggtgc acccgggtcc
      361 cggtcagcgg ccggccgatg gtgacccggc cgccgcgcac ggcgacgatg aacgtcgagt
      421 aggtcgtgtc ctccgacggg ccgtacaggt tcagcacccg ctccacctgt ggatgctcgt
      481 agatccgctc cacgagccgg actttgagcg cctcccccgc caggttgacc gtccgcaccg
      541 acgccggcag cggtccggcg gccagctccg ccatcgtcga ggggacggtg ttgatcagtc
      601 gtacctcgtc ccgggccgcc agctccggca gctccagggc gttggccgcc agcaccaccc
      661 ggccgccgta cgccaggggc acgaacagct cgaacaccga caggtcgaag ctgatggagg
      721 tggccgccag cacgcccgac agctcctcgg cggtgaacac cccgccggcc cactccacca
      781 gcgccacggc gctgcggtgc tcgatggcca ccgccttggg gcggccagtc gagcccgacg
      841 tatagatgag gtaggcgagg ttgccgggca gcaccagcgg ctcggggtcc aattcgctct
      901 ccgcggcggg caccgcggcg aggagctcgc gggtgaccag cgccgtcagg tccgcatcct
      961 ctgccatcag ctccagacgg gcggccggat aggcggggtc cagcggcacg taggcgccgc
     1021 ccgccttgag gattcccagc agcgccacca ccatggcggc ggaccgctcg agccgcacgc
     1081 ctacccggtc ctcggggccg acgcccagac gccgcaggtg gtgggccagc cggttggccc
     1141 ggcggttcag ctcgccgtag ctcagccgct ccgccgccgt caccagcgct tcggctccgg
     1201 gcgtcagggc cgcctggcgc tcgaagagcc ggtgcaggca gagcccctcc cccctctccc
     1261 ggccggtgtc gttccattcc accagcaggg catggcgctc ggccccggcc agcaggggca
     1321 cctccgccgg aggcagcccg ggatctctgg ccacgtcttc cagcaggcgc tccaggtggg
     1381 ccagcagccg gtcgatggtc acgccgtcga acaggtcggt gccgtactcg gcgcccagct
     1441 ccaggccgtc ccgctcctcc cgcacctcga gagtcaggtc gaacttcgcc gtgccggtgc
     1501 ccaccgccag gccgggagcc tccgtcccgg agccgcgcgg cccgcccgtg gccggcagcg
     1561 acagcatcac ctggaagagc ggcgcgtggc tgaggttgcg cgccacccgc agctcctcca
     1621 ccagcttttc gaacggcaga tcctggtggg cgtaggccgc cagcgccgtc tcccggacct
     1681 ggcggagcag gtgccggaag cacaccccgc tcgcgagccc cacccgcagc ggcagcgtgt
     1741 tgacgaagaa gccgatgagg cgctccacct ccagccggtt gcggccggcg atgggcgttc
     1801 ccagcaccag atcgtcctgg cccgcccagc ggcccagcag tgccgcggac gcggccagca
     1861 ggctcatgaa cagcgtcgtc ccctgctcgc ggccgagccg ccgcaagccg gcttcgaggg
     1921 gtgcggacag ccgccggata tgccgcgctc cgcgatggct cggctggacg gggcggggcc
     1981 tgtccgccgg cagctccaac gacgccggcg cgccggcgag ttggccgcgc cagaaggcga
     2041 gctgacgctc cagctcctcg ccggccagcc aggcccgctg ccagacggcg aaatcggcgt
     2101 actggaccgg caactccggc agcggcgacg gccggccggc cccgaaggcg gcatagaggg
     2161 ctgtcacctc ctccaccagc agatccatcg accaggcgtc cgacacgatg tggtggaggg
     2221 tcagcagcag ccaccgcgcg ggtccgtccg cgccgacgag agtggcgcgc agcagcggac
     2281 cggcggccag gtcgaagggg cgcgtcgctt cctcgcccgc cagccgcaac gtctcggcct
     2341 ttcggcggcc cgccggcagc ggcgccaagt ccaccagcgg cagcgggaag ctcccggcgg
     2401 ccgacacccg ttgcaccggc ccgccgtccg tcagatggaa cgtggtgcgc agcgcttcgt
     2461 gccggcgcac cacctcgccc agggcgcggg ccaggaccgc ggcatcgacg ccgtcggcca
     2521 gccagaggga gaggggcatg ttgtagacgg cgctccccgg ctgcaaccgg tcgaggaacc
     2581 agagccgttc ctgggcgaag gagaggggca gaggcccggt ctgttcggcc gggaccagcg
     2641 gcggcgcggc ggcgaaccgg cccgccgccg tcgcggcctc gaccgccgtc gccagcccgg
     2701 ccacggtggg cgcctcgaag agcatccgca gcggcagctc gacgccgaac gcctcgcgca
     2761 cccgcgacat gacccgcgtc gccagcagcg agtgaccgcc gagctcgaag aagctctcgt
     2821 ccgcccccac ccgctcggcg ccgagcacgg cggcgaagat cccggccagc agctcctcca
     2881 ccggcgtgcg gggtgccgcg gcgggggcgg cttcggtcac gggtgcgggc agggcacggc
     2941 ggtccacctt gccgttgggg gtgagcggca gggcgtccag gagcacgaaa gcggccggca
     3001 ccatgtgctc cggcagccgc tcggcgagga agcggcgcag ctccgcggcg ccggggcgcg
     3061 gctcggcggt ggcggcatag gccaccaggc ggcggtcgcc gggcgtgtcc tcccgcgcca
     3121 gcaccgccgc ggcgccgacc gccgggtggg cctgcagcgc cgcttcgatc tcgccgagct
     3181 cgatgcggaa gccgcgcacc ttgacctggt gatcgatgcg tcccaggtac tcgatgcgcc
     3241 cgtcgggccg gtggcgcgcc aggtcgccgg tgcggtagag gcggtcgccg ggccagccgg
     3301 aaggatcggg cacgaagcgc tcggcggtca ggtccggacg gcccaggtag ccccgggcca
     3361 gccccgcgcc ggcgatgcag agctcaccgg gcacgccggt gggcaccggc tcgctgccgg
     3421 gtcccagcag gtggacccgg acgttgggca gcggacggcc gagcgccggg cggacggtcg
     3481 ccaccggcat cacggtggcg tccaccgtgc actccgttgg gccatacacg ttgtagacgc
     3541 tgcccggagc cgccgaagcg agggacgtcc agagatcctc cgggatgtcc tcgccgccca
     3601 ccaggacccg gcgaggggcg gcggccgtct ccgtcaggcc cgcctccagc agcagccgga
     3661 gctggccggg ggtgcagtcc aggacgtcga cccggcggct ctccaggagg gcgagcagac
     3721 ggccggggtc gagacgggac tcctcgtcca ccaggaccag ggtgtggccg gcgaggacct
     3781 ggatccactg cttcaccgag gcgtcgaagg ccaggggggc gttgaccgcc acccggagcg
     3841 gagcgtcgag cccggcgtag acggtggcgc ccagggacgc gagcaggttc accaccgacc
     3901 cgtgccgcac catcaccccc ttggggacgc cggtggagcc ggaggtatag atgacgtagg
     3961 ccaggtgctc cggcaacgac aggggctccg ggtcggtgac gggctcgtcc agagcgccgt
     4021 ccagcaagac gagagggagg ctggacggcg gcagcgccgc ggccgggccg gagcgcgtca
     4081 ccaaggcggc gatcccggca tcttccagca ggaaggcgag acgctcggcg ggataggagg
     4141 gatcgagcgg cacgtaggct ccccccgcct tgaggatgcc cagcaggccc accaccatgg
     4201 ccgccgagcg gtccatgcac aggccgacgc ggctctccgg gccgacgccg agccgccgca
     4261 ggcggtgggc cagggcgttg gcgcgctggc ggaggtcctg gaaggtgagg cgctcgtccc
     4321 cactctccac ggccggcgac tccggagtgc gggaggactg gaggtcgaag agctggtgga
     4381 ggcagagcgc cggtacgccc atgccgccgg tgccgctgat gctggcagtg tcgttccact
     4441 cccgggcgag ctcgtggcgc tcggcctcgg tcagcagagg gagatcggag agccggcgct
     4501 ccggctccgc caccgccgcc gccaccagag tgctcaggtg gtgcaggagc ctctccgccg
     4561 tgccggcgtc gaagagctcg gcgcggtaga ccagagagag atgcccgcct tgctcgccgc
     4621 cggcggccgt gaggctgagg tcgaagcggg ccgtcgccgc ctcgtccggc agcggcctgc
     4681 cgtcctctag gccgccggag ggggtgctct ggaaggtgaa catcacttgg aagagcggcg
     4741 tgtgggagag gctccgctgc ggctggagct cttccaccag gcgctcgaag gggaggtcct
     4801 ggtgcgagaa ggcgccgagg gtcacctccc gggtccgccc gaggagcgcg gcgaaggagg
     4861 gatcgcccga caggtccgcg cgcagcacca gcgtgttgac gaacagtccg atcagccgct
     4921 ccgccagcag gtgggtgcgg ccggccaccg gtgtgccggc gctgacgtcg gtctgtccgg
     4981 agtaacgcgc cagcagcatc tggaaggccg ccagcagcgc catgaacggc gtcacgccgc
     5041 gctccagggc gaggcgcgcc agggagcgcc agacctcttc gggcagcggc agggagcgga
     5101 cgccgccgcg ggcggactgc accgccggcc gcggccggtc ggtgggcagc tcgagcagcg
     5161 gcggcgcgcc ggccaggcgc ccgcgccagt aggacaggtg ggctgcgagc cgatcctcgg
     5221 tcagggtgcg gcgctgccag acggcgaagt cggcgtactg gaccggcagc tccggcagag
     5281 gcgaggcctc gccccgcgcg aacgcctgaa ccaaggcggt cacctcctcc gccagcaggt
     5341 ccatggacca gccgtccgcg acgatgtgat ggacggtgaa gagcaaccac tgccccggct
     5401 cgcccaactg gagcagggtg gtccgcagca gcgggccggc ggacaggtcg aaggggcggt
     5461 ccgcctcgtg gccggcgagg cgcgaggctt cggcgtcccg tggcgccgcg gggagggccg
     5521 agaggtccac ccggaccagg ccccagccag tggcggcggc ggatggtccg atccgctgga
     5581 ccggccggcc gctttccatg gggaaggtgg tgcgcagcgc ctcgtggcgg cggatcacct
     5641 cgcccagcgc gctctccagc aggcgcgtct cgaggccggc ctcgagacgc agggccagcg
     5701 ggatgttgta ggcggcgctg ccgggctcga gctggtcgag gaaccagagc cgctcctggg
     5761 cgaaggagag gggcagcggc agctcccgcg ctgccggagc cagaggctcc agcggcggga
     5821 gagcctggaa gggggcttcc ccggacgccg acaccaccgc agccagcccg gacacggtgg
     5881 gcgcctcaaa gagtgcccgc agcggcagct cgatgccgaa cgcctcgcgc acccgcgaca
     5941 tgacccgcgt cgccagcagc gagtgaccgc cgagctcgaa gaagctctcg tccgccccca
     6001 cccgctcggc gccgagcacg gcggcgaaga tcccggccag c
//
LOCUS       DASPJI010000593        16896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_208315, whole genome shotgun sequence.
ACCESSION   DASPJI010000593 DASPJI010000000
VERSION     DASPJI010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16896)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16896
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208315"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VEL74_16550"
     CDS             <1..1062
                     /locus_tag="VEL74_16550"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HYG64188.1"
                     /translation="GSGGVGKTTLAASISVLAARTGRDTLVMTFDPSHRLKDALGVGE
                     EARFQEVPVDFDAPGKLHASLLDASQTFDRLIERYAPDEASRRRILGNRFYQHLSGTL
                     GGILEYMAVERLFEVALEGRYQQVILDTPPTRQALDFLEAPARIMNFLDSGALRVALK
                     PWFDEEGHLKAASRWGVLGRRAERFLDEVVGMDLLRDMAEFFQAFTPLFGGFRERAQK
                     VEELLRSTRTRFVLVSGPGEERIPDTLFFARRLEQAGYHLGPIIVNRVHPRFLVDNAP
                     PPSTDPCRPDGWELLGWFGERDRKGLVEMAKLLSREQPLVDVPLLPDEPTDLPALEAL
                     GREVESRLEQWARYLSRSS"
     gene            1143..1424
                     /locus_tag="VEL74_16555"
     CDS             1143..1424
                     /locus_tag="VEL74_16555"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HYG64189.1"
                     /translation="MSRTIGVVELQEHFDSVLHKVAEEHVPYVLTRDSRPEAALIPYE
                     EFLRFQAFREKEVLDLFQRAQARMTEKNAGYSDEEIAADVQAARNECPD"
     gene            1412..1843
                     /locus_tag="VEL74_16560"
     CDS             1412..1843
                     /locus_tag="VEL74_16560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HYG64190.1"
                     /translation="MPGLIRAVIDTSILVRAFLKPQGTVGPILDRLAEGRYAFVSSRP
                     LLDELIDVLNRPRLRMKYSLRKEDVEDLLKLLLLRGEVVEPARSIVACRDPKDDKVLE
                     AAVEGRAQFIVTGDEDLLVLSPFESILMVGPSEFLHSLPSP"
     gene            2005..2484
                     /locus_tag="VEL74_16565"
     CDS             2005..2484
                     /locus_tag="VEL74_16565"
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HYG64191.1"
                     /translation="MIRLIKRYESRKLYDTEESRYVSLDEIAGWVRQGQEVRVVDNAT
                     SADVTSQTLTQIILDEGRKGTSFIPSDLLHELVRIGEKAVTTGMEQVQSGVDRLVQAS
                     IDRLGPVRRAREEMASLRARLEQLEHSLAHLESDSPAASAAPEPAVVIVGTTDPDIK"
     gene            2496..2960
                     /locus_tag="VEL74_16570"
     CDS             2496..2960
                     /locus_tag="VEL74_16570"
                     /inference="COORDINATES: protein motif:HMM:NF017415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HYG64192.1"
                     /translation="MSQAEINTGMPGKGLLAAGRSLWLAGLGAVAKTADADRESRVLF
                     DRLVEKGRPVEERGRETVEGWSERTGETFRELGQLVRDTVEYESKGLLKRFGIATRDD
                     FKVLSNRLDILARNVDEMVARWQVERAEAMSPAAPAEIILTTATGTPIAKEA"
     gene            2965..3453
                     /locus_tag="VEL74_16575"
     CDS             2965..3453
                     /locus_tag="VEL74_16575"
                     /inference="COORDINATES: protein motif:HMM:NF017415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HYG64193.1"
                     /translation="MDTTNPTDSQQDKTLQDDLKESAHRIWLAGLGALAAAEQEGAKM
                     FNRLVDRGRDFESKGRTEARDTYEKTRTEVKDTYDKARTQAQATWENVGDKVDEVVTA
                     ALHRLGVPTREEIHTLTLRVEELNAKVESLKPRVTPAAAGEPAVITPAEATPNPEAKI
                     IV"
     gene            3465..4829
                     /locus_tag="VEL74_16580"
     CDS             3465..4829
                     /locus_tag="VEL74_16580"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYG64194.1"
                     /translation="MGDEENNGSADAGTRDRKIGLALAAGAPEGAIYEIGALRAIDEA
                     LDGLDLNNLHVYVGVSAGAFLSANLANNLTTAQMCRAIVSHEPGEHPFRPETFLTPAW
                     SEFLRRGGMVPGLVLDAIREWITHRRDYTLIESLTRVSRALPVGLFDNSAVRDYLEKI
                     YSIKGRTDDFRKLGKHLVVVATDLDSGKPARFGEPGLDHVPISLAVQASSALPGLYPP
                     VEIDGRHYVDGVLLKTLHASVALEAGAELVICVNPIVPVDTVRAVEQGLMKRSNLLNR
                     GLPSVLSQTFRTMVHSRLEVGIASYETKFQGADVVLIQPKRDDYLMFFTNIFGFSERR
                     AVCEYAYNSTRRDLLARYDELAPKLARHGITLRRDVLEEPRKLWDGVVLPPAALKEER
                     RKGTGGSGHDTVRRLDDVLARLEKLVAEREAEAAANPQATPQTPQSTEALVPIESGLP
                     AVVN"
     gene            complement(4889..6934)
                     /locus_tag="VEL74_16585"
     CDS             complement(4889..6934)
                     /locus_tag="VEL74_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYG64195.1"
                     /translation="MKRKLPASAVAALTLAFLLAGDGGALVAQPAAPEPDMVPVPDSI
                     IARNVPPIPQERVADLIPYENLRGAYISDWHPTERRMLVATRFGESLQLHELSMPLGA
                     RTQITFYRDTVPSGAYRPNDPDRLFFALDEGGAENYQAFLLDRKTGKARRFTDGKHRY
                     EEALWSYDGKLLTYVSNSRNGRDFDLYVTDPAVAGSERRVVELEGSWVPLDWSVDGKR
                     ILLRQGVSSTEAYLHAVDVATGALTPLTPKAGPDAPQVAWEGGRWSKDGRYVYTTTDK
                     DGEFRRLVRLDPATGATTVVSGDVPWDVQTFELSHDGKVLSFFVNDDGVSRLHMINTA
                     TGRSLPTPELPPGVAGKLLPRPGSHEVGFNLSWARSPSDVYSYDPDTGRLARWTASEV
                     GGLDTDTFAVPKLVRFPTFDKDEKGARRTIPSFIYRPPADRFPGKRPVYINIHGGPEA
                     QSLPNFVGSLNYLINNLGVAVVYPNVRGSSGYGKSFLKLDNFEKREDAVKDIGALLDW
                     IATQPDLDASRVMVTGNSYGGYMVLASLVHYGERLRCGYDAWGISNILSFLQNTQEYR
                     RDQRRVEYGDERDPKMRQFLEETAPASRVDRINRPLLVAQGANDPRVPMSESDQIVAA
                     LQNKGVPVWYVVAKDEGHGFGKKSNADYLRVVHFEFVRRCLLGSGDDLAAPKPQNPS"
     gene            7268..>16896
                     /locus_tag="VEL74_16590"
     CDS             7268..>16896
                     /locus_tag="VEL74_16590"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64196.1"
                     /translation="MNGHLPDATTLIDLLRGRAATSPDRPAFTFLVDGEVEGGRLTYG
                     ELDRRARALAVRLQERYAPGERVLLLFAPGLDFVAAFFGCLYAGTIAVPVYPPRAQRD
                     QPRLRSILADASPRAALTTSDILARVRSLAEGFPELLDLDWVATDEVTDEGAGLWRVP
                     GIGPGDVAFLQYTSGSTGTPKGVMVGHGNLLHNEEMIRRSFEQSEASVVVGWLPLYHD
                     MGLIGNVLQPVYNGGSCVLMSPAAFLQRPLRWLEAIHRYRGTTSGGPNFAYDLCARRA
                     LAAPPAELDLSSWTVAYNGAEPVRAETLDRFSQVFEPHGFRREAFYPCYGLAEATLFV
                     SGGTRGVGPVVAPREEGATPLVGCGRSWMEQEVAVVDPATGERRPDGGVGEIWVGGPS
                     VALGYWNRPEATEETFRARLAGDGAGPFLRTGDLGFFQGGELFVTGRIKDLVIIRGRN
                     HYPQDIELTAEASHASLKPGGGAAFAVDVDGEERLVVVHEVERRADGDPDAACAAVRQ
                     AVAAAHEVQVHEVVLIRVGTLPKTSSGKVQRHATRAAYLAGSLHVVARSAVAAAAALE
                     PLPGGAEGEGFRRGDLLALPPADREAAVRELLARQVARLTGARPEDLRVDLPLAAAGL
                     DSLAAIQLGHAAETALGVPVAMSDLLEGVSLSGLVRRLLRELEEGSGETRPALTPDGG
                     TEGLHRTTYGQRALWFLERMGADGAYNVVAAMRLRSAVGEADLHEALRGLVRRHPALR
                     TTLEPADGAALQRVHEWLEPGFTVEDGAEAAEAADDADWIAASAYAPFDLEHGPLLRL
                     HVRRAGAAGSDGAEGPLLVLAVHHTVSDFWSLGVMVRDLAALAAGQAPPPEPALRATE
                     FARWQERLLAGAEGERLWEAWRAELAGRTPNLDLPLDRPRPPLQTYRGGALPVRLGRL
                     GGERLARLLETGREAGATPFMTLLAVFQTLLHRYSGQDVVVCGSPTAGRTAAELARVV
                     GYFVNPVVLRGDLAGEPSFSELLGRVRRTALAAFSLQEMPFPLLAERLQPQRDPSRSP
                     LFQVMFVLQKAHDPELRALDAFALREPGHRLEIGGLVLESVEIPDRRPQFDLALTAVE
                     VDGEVRGSLEYNADLFDAATVERMARHLENLAGAAAVSPGLPVGLLPLMDESESWQAL
                     RGWNQTAREYPASTLHGLFEEQAERTPDAVALVCEDEQVTYRELRDRAWRLARHLEKM
                     GVGPDERAGVFMERSNDMVVALFGALAAGGAYVPLDPSYPKDRLAYMVEDSRVPVVLS
                     QRHLLSDLPETSRGSARRLCVDADWPLVAGEPAVRLPREPAGGNLAYVIYTSGSTGRP
                     KGAMNTHAGIVNRLLWMQEEYGLDGTDRVLQKTPFSFDVSVWEFFWPLLAGATLVMAR
                     PGGHRDSAYLVDEIARRGITTLHFVPSMLQVFLEDAARQPCASLRRVICSGEALPAEL
                     AERFFERLDCSLHNLYGPTEAAVDVTYWACEPGGRRSVPIGRPIANIQIYLVDRRFEP
                     VPVGIPGELLIGGVGLARGYHGLPELTAERFVPDPFAAEPGARVYRTGDLTRRGVDGE
                     VEFLGRLDFQVKIRGFRIELGEVEAALAQHPAVREAVVLAHEAATGDRRLIAYVVPRR
                     EPAPTPAELQAALRERLPDYMVPAWIGVLESMPLNPSGKVDRKALPAPDTGMAAAEYV
                     APRTPVEEVLAGIWSDVLGVERVGVHDGFFDLGGHSLLAARVVSRVREALGVDLPLRT
                     MFDAPQVARFAATVEAARQAGLADRLPPLVASHHTGPAPLSFAQERLWFLQQLAPESP
                     AYHLPGAVRLEGRLDIPALARALTEVAARHDILWTAFSPSQDGDSVAQTVLPPGPLPL
                     PVVDLGLLPEAVRWDEERRLARLEGRRLFDLSRGPLVRSVLLRLGAAEHVLLVTLHHI
                     AADGWSLGLLVRELNALYAASLGGSASPLPALPVRYEDFARWQRSWPAAFLAAELAHW
                     TARLEGAPAVLALPADRPRPGTRRERGAKRPVRIGGETAAAVRVLARRTAASPFMVLL
                     AALDALLQRLTGQSDLVVGSPIAGRDRVELEGLIGCFVNSLALRAEADPERPFRDLVA
                     GVRQAALDAYAHQELPFGRLVEALQPERNLAYTPLFQVLFVLQNSPLPFLDLPGLRLG
                     WSEVETGTAKFDLTLSLEEGEDGWSGTLEYDADLFDATTAERLVERYGLLLAGALDRP
                     EAPLAELPALTAAERQALLVEWNDTAALEDGADTLPALFAAQAARTPDAPALRCGAEV
                     LSFRELDRRATRLARCLRRMGIGPEERVGVLLPRTPRLVVALLAVLRAGGVYVPLDPA
                     YPRQRLALMLEDSGARGLLTERALTELAGHRVDWALCLDEMPEESLEEMADAGAPEAG
                     GEVEVLGDHLAYLIYTSGSTGRPKAVAIRHRSAAVLLCWARDTFREDERRAIAASTSV
                     CFDLSVFELFTPLAWGGTVILLGNALDLAALPAGCEVTLVNTVPSAMTELVRLGAVPS
                     SVRTVALAGEPLPRTLADSLHGLGHVGRVANLYGPSEDTTYSTFSFVPTEGGAPAIGR
                     PLAGTRVYLLDASGRSAPPGVAGEICLGGEGLARGYLGRPDLTAERFVPDPVSGGGRL
                     YRTGDLGRYRADGEIEFLGRIDHQVKVRGFRIELGEVEEALLRLPGVAEAVVVVRGDA
                     TSDRRLVGYVGTGSGAAQPEERLRSLLLESLPAYMVPSSLVVLEALPRTPNGKVDRKA
                     LPEPEAPGAGAEPAGVFVAPRTALEEILAGIFAEVLGAERVGVRDDFFALGGHSLLAA
                     RVASRLRQALGVELPLRRLFGSPTVEALAAAVEELRGSGEASSQPLPPLATGPRSGLL
                     PLSYAQERLWFLEQLEPGSAAYNVPTAVDLEGRLDVVALRQSLEVVVARHESLRTVFT
                     AMAGGPAQRVLKPMPVPLPEVDLTGLPHPEREARRLGAEEAARPFDLTRGPLLLRARL
                     LRLDGQRRRLLLTLHHIVSDGWSMGVLVREIGALYGQGEQAGLPELPVQYADYAAWQR
                     SWLEGETLEREAGFWRQRLAGAPPVLELPTDRPRPAVCSGRGGLRHLELAPELVDGVT
                     RLARRQGATPFMVLLAGFYALLHRVSGVEDLVVGSPVAGRDRSETEGLIGFFVNTLVL
                     RAAVSGEVSAGELVRQARETVLEAQAHHWLPFEKLVELLQPARDLSYTPVFQVMLAYQ
                     NDPLPPLDLPELRLRPVEADGGTAKFDLTLSLRP"
BASE COUNT         2306 a         5806 c         6243 g         2541 t
ORIGIN      
        1 ggctcgggcg gcgtggggaa gaccaccctg gccgcctcga tcagcgtgct cgccgcccgc
       61 accggccgcg acacgctggt gatgaccttc gatccgtccc accggctcaa agacgccctg
      121 ggagtggggg aggaggcgcg cttccaggag gtgccggtcg acttcgacgc gcccggcaag
      181 ctccacgcga gcctgctcga cgccagccag accttcgacc gcctgatcga acgctacgcg
      241 ccggacgagg cgtcgcgccg gcgcatcctc ggcaaccgct tctaccagca cctgtcgggg
      301 acgctggggg ggatcctcga gtacatggcg gtggagcgcc tgttcgaagt ggcgctggag
      361 ggccgttacc agcaggtgat cctggatacg ccccccaccc gccaggcgct ggacttcctc
      421 gaggcgccgg cccgcatcat gaacttcctc gacagcggcg ccctgcgcgt ggcgctcaag
      481 ccctggttcg acgaggaggg gcacctcaag gccgcctcgc gctggggcgt cctgggccgg
      541 cgggccgagc gcttcctcga cgaggtggtg gggatggacc tgctgcgcga catggcggag
      601 ttcttccagg ccttcacccc gctcttcggc ggcttccgcg agcgggccca gaaggtggag
      661 gagctgctgc gctccacccg cacccgcttc gtcctggtct ccggccccgg cgaggagcgc
      721 atccccgaca ccctcttctt cgcccgccgg ctggagcagg ccggctacca cctcggcccc
      781 atcatcgtga accgggtcca tccgcgcttc ctggtggaca acgcgccgcc gccctcgacc
      841 gacccgtgcc gtcccgacgg ctgggagctc ctcggctggt tcggcgagcg cgaccgcaag
      901 ggcctggtgg agatggccaa gctcctctcc cgcgagcagc cgctggtgga cgtccccctc
      961 ctcccggacg agcccaccga cctccccgct ctcgaagctc tcggccgcga ggttgagagc
     1021 cgcctcgagc agtgggctcg gtacctgagc cggtcctcgt aggggcccct cgaccgccga
     1081 gccgggaggg tagcgcacat tagtccaagt tggactatgc tctccgtgga gaggatcata
     1141 cgatgagcag aacaattgga gtcgtcgaat tgcaggagca cttcgactcc gtgctccaca
     1201 aagtcgccga agagcacgtc ccgtacgtcc tcaccaggga cagccgtccc gaggctgccc
     1261 tgatccccta cgaagagttc ttaaggtttc aggcatttcg cgagaaggag gtcctcgacc
     1321 tcttccagcg tgctcaagcc cgcatgacag agaagaacgc cggctactcg gatgaggaga
     1381 tcgccgcgga tgttcaggcg gcccgaaacg aatgcccgga ctgattcggg ctgtaatcga
     1441 cacgagcatc ctcgtccgcg cgttcctcaa accccagggg acagtcggtc ccatcctgga
     1501 ccggctcgcg gagggccgtt atgccttcgt ctcttcacgt cccctcctgg acgagctgat
     1561 cgatgtcctg aatcgaccca gactgaggat gaagtactcg cttcggaaag aggatgtcga
     1621 agatctcctc aagttgctgc tcctgcgtgg cgaggtcgtg gaaccggccc ggagcatcgt
     1681 ggcctgccga gatcccaagg acgacaaggt ccttgaagcc gccgtggagg gcagggctca
     1741 gttcattgtc acgggcgacg aagacctcct cgtcctgagc cccttcgaga gcatcctcat
     1801 ggtgggacct tccgagttcc tccatagcct gccctctcct tgaacccctc ggccctgcca
     1861 cggcgtcttg ccgcgccgcg tcaaatcctc ttgacatcct ttgccgcagt gcggtaaatt
     1921 tcctctcgga cgcggcagac cccttgcaat ggtgtgcatc ggggaggtgg ggtgccgcgc
     1981 aaccaccgag acgaggagag atccatgatc cggctgatca aacgctacga gagccgcaag
     2041 ctctatgaca ccgaagagag ccgttatgtc tcgctcgacg agatcgcggg ttgggtgcgg
     2101 caggggcagg aggtgcgggt ggtggataac gccacgagcg ccgacgtcac gtcccagacg
     2161 ttgacccaga tcatcctcga cgagggccgc aagggtacgt cgttcatccc cagcgacctg
     2221 ctgcacgagc tggtgcgcat cggcgagaag gcggtgacca ccggtatgga gcaggtgcaa
     2281 agcggcgtcg accggctggt ccaggcgtcc atcgaccggc tcggtccggt gcggcgggcg
     2341 cgcgaggaga tggcgagcct gcgggcgcgc ctcgagcagc tcgagcattc tctggcccac
     2401 ctggagagcg acagccccgc ggcgagcgcg gctccggagc cggcggtcgt gatcgtgggg
     2461 acgacggacc ccgatatcaa gtgatggaga acgcgatgag ccaggctgaa atcaacaccg
     2521 gcatgcccgg gaagggcttg ctggccgccg gccgcagcct ctggctggcc ggcctcggcg
     2581 cggtggccaa gaccgccgac gcggaccggg agagccgtgt ccttttcgac cgcctggtcg
     2641 agaaggggcg ccccgtcgag gagcggggcc gggagacggt ggaggggtgg agcgagcgca
     2701 ccggcgagac gttccgggag ctcggccagc tcgtgcggga cacggtcgag tacgagagca
     2761 agggcctgtt gaagcgcttc gggattgcca cgcgggacga cttcaaggtg ttgtcgaacc
     2821 gtctggacat cctcgccagg aacgtcgacg agatggtggc gcgctggcag gtcgagcggg
     2881 ccgaggcgat gtcgccggca gcgccggcag agatcattct gaccaccgcg acgggaacgc
     2941 ccatcgcgaa ggaggcatag gaccatggat accaccaacc cgacggacag ccagcaggac
     3001 aagaccctgc aggacgacct caaggagtcg gcgcaccgga tctggctcgc cggcctcggc
     3061 gccctcgcgg cggcggagca ggaaggcgct aagatgttca accgcttggt ggaccgcggc
     3121 cgcgacttcg agtccaaggg ccgcaccgag gcccgggaca cctacgagaa gacccggacc
     3181 gaggtcaagg acacctatga caaggcgcgc acccaggcgc aggccacctg ggagaacgtc
     3241 ggcgacaagg tcgacgaggt ggtgacggcg gccctccacc ggctgggcgt gcccacccgc
     3301 gaggagatcc acaccctgac gctgcgcgtc gaggagctga acgccaaggt ggagtcgctg
     3361 aagccgcggg tcaccccggc cgcagcgggc gagccggcgg tcatcacccc ggcggaggcc
     3421 acccccaacc ccgaggccaa gatcatcgtc taggcggggg ccccatgggc gacgaagaga
     3481 acaacggttc ggcggacgcc ggcacccgag atcgaaaaat cggcctggcg ctcgccgcgg
     3541 gagccccgga aggcgccatc tacgagatcg gcgccctccg ggccatcgac gaggccctcg
     3601 acggactgga cctcaacaac ctgcacgtct acgtcggggt gagcgcggga gccttcctct
     3661 ccgccaacct ggcgaacaac ctgacgacgg cgcagatgtg ccgcgccatc gtctcccacg
     3721 agccgggcga gcaccctttc cgcccggaga ccttcctcac tccggcgtgg agcgagttcc
     3781 tgcgccgggg cgggatggtg ccgggcctgg tgctggacgc catccgcgag tggatcaccc
     3841 atcgccgcga ctacacgctg atcgagtcgc tgacgcgggt gtcgcgcgcc ctccccgtgg
     3901 ggctgttcga caacagcgcg gtccgcgact acctggagaa gatctacagc atcaaggggc
     3961 gcacggacga cttccggaag ctgggcaagc atctggtggt ggtggcgacc gacctcgatt
     4021 cgggcaagcc cgcccgcttc ggcgagccgg gtctcgacca cgtgccgatc tcgctggcgg
     4081 tccaggcgag ctcggccctg ccggggctct acccgccggt ggagatcgac ggccggcact
     4141 atgtggatgg cgtcctcctg aagaccctcc acgcgtcggt ggcgctcgag gcgggggccg
     4201 aactggtgat ctgcgtcaat ccgatcgtcc cggtggacac ggtccgcgcc gtggagcagg
     4261 ggctgatgaa gcgcagcaac ctgctgaacc gaggcctgcc ttcggtgctg tcgcagacgt
     4321 tccgcaccat ggttcactcg cggctcgagg tgggcatcgc ctcctatgag accaagttcc
     4381 agggtgccga cgtcgtgctc atccagccga agcgcgacga ctacctgatg tttttcacca
     4441 acatcttcgg cttctcggag cgccgggcgg tctgcgagta cgcctacaac tccacgcggc
     4501 gcgacctgct ggcccgctac gacgagctgg cgccgaagct cgcccggcac ggcatcacgc
     4561 tgcggcggga cgtgctcgag gagcccagga agctgtggga cggcgtcgtc ctgccgccag
     4621 ccgccctcaa ggaggaacgg cggaagggga ccggggggtc ggggcacgac acggtccgcc
     4681 gtctggacga cgtgctggcg aggctggaga agctggtcgc ggagagggag gcggaggccg
     4741 ccgcgaaccc gcaggccact ccgcagaccc cgcagagcac cgaggccctg gtgcccatcg
     4801 agtccggcct gccggccgtg gtcaactgac gcgatccatt ccgatcgaaa gaaacggcgg
     4861 agagggggac ctctccgccg ttcgcgcttc aggagggatt ctgcggtttc ggcgccgcga
     4921 ggtcgtcccc cgagccgagc aggcagcggc ggacgaactc gaagtgtacg acccgcaggt
     4981 agtcggcgtt gctcttcttg ccgaaaccgt gtccttcgtc cttcgccacc acgtaccaga
     5041 ccggcacgcc cttgttctgc aacgcggcga cgatctggtc cgattcggac atcggcacgc
     5101 gggggtcgtt ggcgccctgg gccacgagga gcggccggtt gatccggtcg acccggctgg
     5161 cgggcgcggt ctcctccagg aactggcgca tcttcgggtc gcgctcgtcg ccgtactcga
     5221 cccggcgctg gtcgcggcgg tactcctggg tgttctgcag gaagctcagg atgttgctga
     5281 tcccccaggc gtcgtagccg cagcgcagcc gctcgccgta atggaccagc gaggcgagca
     5341 ccatgtagcc gccgtaggag ttgccggtca ccatcacccg ggaggcgtcg aggtcgggct
     5401 gggtggcgat ccagtcgagc agcgcgccga tgtccttgac cgcgtcctcg cgcttctcga
     5461 agttgtccag cttgaggaag ctctttccat agcccgacga gccgcggacg ttgggataga
     5521 ccacggcgac ccccagattg ttgatcaggt agttgaggct tcccacgaag ttggggaggg
     5581 actgcgcctc gggcccgcca tggatgttga tgtagaccgg gcgcttgccc gggaagcggt
     5641 cggccggcgg ccggtagatg aaggagggta tggtgcggcg cgcccccttc tcgtccttgt
     5701 cgaaggtggg gaagcggacg agcttcggca cggcgaaggt gtcggtgtcg aggccgccca
     5761 cctcgctggc ggtccagcgc gccagacggc cggtgtccgg gtcgtagctg tagacgtccg
     5821 acggtgagcg tgcccagctc aggttgaagc cgacctcgtg cgagccggga cgggggagga
     5881 gcttgccggc gaccccgggg ggcagctcgg gcgtgggaag agaccggccc gtggccgtgt
     5941 tgatcatgtg gagcctgctg acaccatcat cgttgacgaa gaaggagagg accttgccgt
     6001 catgggagag ctcgaaggtc tgcacatccc acggcacgtc gccggagacg accgtcgtcg
     6061 cgccggtggc cgggtccagg cggaccaggc gccggaactc gccgtccttg tcggtggtgg
     6121 tgtagacgta gcggccgtcc ttggaccagc ggcccccctc ccaggcgacc tggggcgcgt
     6181 ccgggccggc cttgggcgtc agcggcgtca gcgcgccggt ggcgacgtcg accgcgtgaa
     6241 ggtaggcctc ggtggacgag actccctggc ggagcaggat ccgcttgccg tcgacgctcc
     6301 agtcgagcgg cacccagctc ccctcgagct ccaccacccg ccgctcgctc cccgccaccg
     6361 ccgggtcggt gacgtagagg tcgaagtcgc ggccgttgcg ggagttgctg acgtaggtca
     6421 ggagcttgcc gtcataggac cagagggcct cctcataccg atgcttgcca tcggtgaagc
     6481 ggcgggcctt gcccgtcttg cggtcgagca ggaaggcctg gtagttctcg gctcccccct
     6541 cgtccagggc gaagaacagc cggtcggggt cgttgggccg gtaggcaccg ctggggaccg
     6601 tgtcccggta gaaggtgatc tgggtgcggg cgcccagcgg catggacagc tcgtggagct
     6661 ggaggctctc gccgaagcgg gtggcgacga gcatgcggcg ctcggtggga tgccaatcgc
     6721 tgatataggc gccccggagg ttctcatagg ggatgaggtc ggcgacccgt tcctggggga
     6781 tgggtggcac gttgcgggcg atgatgctgt ccggcaccgg gaccatgtcc ggctcgggcg
     6841 ccgcaggctg agccaccagg gcccctccgt cccccgccag caggaaggcc agtgtcaaag
     6901 cggcgacagc cgaggccgga agttttcttt tcatttcaga tctcccatgc gttttacgtg
     6961 caacaaaggc aaagcaacaa tggcgcttcg cgcttccgat agtaacacgc taattaataa
     7021 tatccagcgt atcgttaagg cttcgtcgcg agtgcggagg tcccgtccac aaagcttatc
     7081 agggagagtc cggacctgga gagacgtttc catggccgct gcttgacctc gcctccgcgc
     7141 cggatttctg ataaattatc cactggcaac ccgacggcag cctggtctca acggtctctc
     7201 tcctcctcgc tccggcttta catggttgtt aatgagattc cgctgaaata gaggttgagt
     7261 tattgcgatg aatggccacc ttcctgacgc gacaactctg atcgatcttt tgcgtggccg
     7321 cgccgccacg tcgccggacc gtcccgcctt cacattcctg gtggacggtg aggtggaggg
     7381 cggccgtctc acctatggcg agctcgaccg gcgggcgcgg gccctggcgg tccgcctgca
     7441 ggagcgctac gctcccggcg agcgggtgct gctgctcttc gcccccgggc tcgatttcgt
     7501 cgcagcgttc ttcggctgcc tctatgccgg caccatcgcc gtcccggtct atccgccgcg
     7561 cgcccagcgt gaccagccgc ggctgcgctc gatccttgcg gacgcctcgc cccgcgcggc
     7621 tctgaccacc tccgacatcc tggcccgggt gcgctccctg gccgaggggt tccccgagct
     7681 cctggacctc gactgggtgg cgacggacga ggtgacggac gagggggccg gcctctggcg
     7741 cgtccccggc atcggccccg gcgacgtggc cttcctgcag tacacctccg gttccaccgg
     7801 cacgcccaag ggggtgatgg tcggccacgg caacctgctg cacaacgagg agatgatccg
     7861 ccgctcgttc gagcagtcgg aggcgtcggt ggtggtgggt tggctgccgc tctaccacga
     7921 catggggctg atcgggaacg tgctgcagcc cgtctacaac ggcggctcgt gcgtcctgat
     7981 gtcgccagcg gccttcctgc agcgcccgct gcgctggttg gaggcgatcc accgctaccg
     8041 cgggaccacc agcggcggcc ccaacttcgc ctacgacctc tgcgcgcgcc gcgccctggc
     8101 cgcgccgccg gcagagctcg acctctcctc ctggacggtg gcgtacaacg gcgccgagcc
     8161 ggtacgcgcc gagaccctgg accgcttcag ccaggtgttc gagccccacg gcttccggcg
     8221 ggaggcgttc tacccctgct acggcctggc cgaggccacc ctgttcgtca gcggcggcac
     8281 gcgcggcgtc gggccggtgg tggcgccgcg ggaagagggg gcgacccctc tggtggggtg
     8341 cggccgctcg tggatggagc aggaggtcgc cgtcgtcgac cccgccaccg gcgagcgccg
     8401 cccggacggc ggcgtcggcg agatctgggt gggggggccg agcgtggcgc tcggctactg
     8461 gaaccggccg gaggcgacgg aggagacgtt ccgcgcccgc ctcgccggcg acggggccgg
     8521 gcccttcctg cgcacggggg acctcggctt cttccagggc ggcgagctgt tcgtcaccgg
     8581 ccgcatcaag gatctggtga tcatccgtgg ccgcaaccac taccctcagg acatcgagct
     8641 gacggccgag gcgagccacg cctccctgaa gccgggcggc ggggccgcct tcgccgtcga
     8701 tgtcgacggc gaggagcgcc tggtggtggt ccacgaggtg gagcggcggg cggacggcga
     8761 cccggacgcg gcctgcgcgg cggtgcgcca ggcggtggcc gcggcgcacg aggtccaggt
     8821 ccacgaggtg gtgctgatcc gggtggggac gctgcccaag acctccagcg gcaaggtgca
     8881 gcgccacgcc acccgcgccg cctacctggc cggctccctc cacgtggtgg cgcgcagcgc
     8941 cgtagcggca gcggcggcgc tggagcccct ccccggcggt gccgaggggg agggcttccg
     9001 gcgcggcgat ctcctggccc tgccgccggc ggaccgggag gcggcggtgc gggagctcct
     9061 ggcgcggcag gtcgcgcggc tgaccggcgc gaggcccgag gacctgcggg tggacctgcc
     9121 cctggccgcg gccgggctcg actcgctggc ggcgatccag ctcgggcacg ccgccgagac
     9181 cgcgctcggc gtgccggtcg cgatgagcga cctgctggag ggggtctccc tctccggcct
     9241 cgtccgccgt ctcctccgcg aactggagga ggggagcggc gagacgcgcc ctgcactcac
     9301 cccggacggg gggaccgagg ggctccaccg cacgacctac ggccagcgcg cactctggtt
     9361 cctggagcgg atgggtgccg acggcgccta taacgtggtg gcggcgatgc gcctgcgttc
     9421 ggcggtgggg gaggcggatc tccacgaagc cctgcgtggg ctggtgcggc gccacccggc
     9481 gctgcgcacc accctcgagc cggcggacgg ggcggcgctc cagcgggtgc acgagtggct
     9541 cgagcccggc ttcacggtgg aggacggcgc cgaggccgcc gaggccgccg atgacgcgga
     9601 ctggatcgcc gcgtcggcct acgccccgtt cgacctcgag cacgggccgc tgctgcgcct
     9661 ccacgtgcgg cgtgctggtg ccgctggctc cgacggcgct gaaggtccgc tgctggtgtt
     9721 ggcggtccac cacaccgtct ccgacttctg gtcgctgggg gtgatggtgc gcgacctggc
     9781 ggccctcgcc gccggtcagg cgccgccgcc ggagccggcc ctgcgggcca ccgagttcgc
     9841 ccgctggcag gagcggttgc tcgccggcgc ggagggagaa cggttgtggg aggcctggcg
     9901 cgccgagctg gccggtcgga ctccgaacct ggacctgccg ctggaccggc cgcgaccgcc
     9961 gctccagacc taccgcggcg gagcgctgcc ggtgcggctc ggccgtctcg gcggtgagcg
    10021 gttggcgcgg ctgctggaga ctgggcgcga agccggtgcg acccccttca tgaccctgct
    10081 cgccgtcttt cagacgctgc tccaccgcta tagcggccag gacgtggtgg tctgtggctc
    10141 gcccaccgcc ggccgcaccg ccgccgagct ggcgcgggtg gtgggctact tcgtcaatcc
    10201 ggtggtgctg cgcggcgacc tcgcgggcga gccgtcgttc agcgagctgc tgggccgggt
    10261 gcggcgcacg gcgctggcgg cgttctcgct ccaggagatg cccttcccgc tgctcgccga
    10321 gcggctgcag ccgcagcgcg acccgtcgcg ttcgccgctc ttccaggtga tgttcgtgct
    10381 gcagaaggcg cacgacccgg agctccgggc gctggacgcc ttcgccctgc gcgagccggg
    10441 gcaccggctg gagatcggcg gcctggtcct cgagtcggtg gagatccccg accgccggcc
    10501 gcagttcgac ctggcgctga ccgcggtgga ggtggacggc gaggtccgcg gctccctgga
    10561 gtacaacgcc gacctgttcg acgccgccac ggtggagcgc atggcccgcc acctggagaa
    10621 cctggccggc gcagcggcgg tctcccccgg gctgccggtg ggcctgctgc cgctgatgga
    10681 cgagagcgag agctggcagg cgctgcgcgg ctggaaccag acggcgcggg agtacccggc
    10741 gagcacgttg cacggcctct tcgaggagca ggcggagcgc acccccgacg ccgtggccct
    10801 ggtctgcgag gacgagcagg tcacctatcg cgagctgcgg gaccgggcct ggcgcctggc
    10861 gcgtcacctg gagaagatgg gcgtcggccc cgatgagcgc gccggggtct ttatggagcg
    10921 ctccaacgac atggtggtgg cgctcttcgg agcgctcgcc gcgggcggcg cctacgtgcc
    10981 gctggacccc agctatccca aggaccgcct ggcctacatg gtggaggaca gccgggtgcc
    11041 ggtggtgctg agccagcggc atctcctgtc ggacctgccg gagacatccc ggggatcggc
    11101 gcggcgcctc tgcgtggacg ccgactggcc gctggtagcc ggcgagcccg ccgtccgcct
    11161 gccgcgcgag ccggccggcg gcaacctggc ctacgtcatc tatacatccg gctccaccgg
    11221 ccgtcccaag ggggcgatga acacccacgc gggcatcgtc aaccgcctgc tctggatgca
    11281 ggaggagtac ggcctggacg gcaccgaccg ggtgctgcag aagacaccgt tcagcttcga
    11341 cgtctcggtc tgggagttct tctggccgct cctggccggc gccacgctgg tcatggcccg
    11401 ccccggcggg caccgggaca gcgcctacct ggtggacgag atcgcccgcc gcgggatcac
    11461 caccctccac ttcgtcccct ccatgctgca ggtcttcctg gaggacgcgg cgcggcagcc
    11521 gtgcgcgtcg ctacggcggg tgatctgcag cggcgaggcg ctgccggccg agctggcgga
    11581 gcgcttcttc gagcgcctgg actgcagcct gcacaacctc tacggcccca ccgaggccgc
    11641 cgtggacgtc acctattggg cctgcgagcc gggcggccgg cgcagcgtgc cgatcggccg
    11701 gcccatcgcc aacatccaga tctacctggt ggaccgccgc ttcgagccgg tgccggtcgg
    11761 aatccccggc gagctgctca tcggtggcgt ggggctcgcc cgcggttatc acgggctccc
    11821 cgagctgacc gccgagaggt tcgtccccga tccgttcgcc gccgagccgg gggcgcgggt
    11881 ctaccgcacc ggcgacctga cgcgccgcgg cgtggatgga gaggtcgagt tcctcggccg
    11941 gctcgacttc caggtcaaga tccgcggctt ccgtatcgag ctgggggagg tggaggcggc
    12001 gctggcgcag cacccggcgg tgcgcgaggc ggtggtgctg gcgcacgagg ccgcgaccgg
    12061 cgaccggcgg ctgatcgcct acgtggtccc ccggcgtgag ccggcgccta cgccggcgga
    12121 gctacaggcg gcgctgcggg agcgcctgcc ggactacatg gtgccggcgt ggatcggagt
    12181 gctggagtcg atgccgctca accccagcgg caaggtggac cgcaaggcgc tgccggcgcc
    12241 ggacacgggc atggcggcgg ccgagtacgt cgccccgcgg acgccggtgg aggaggtgct
    12301 ggccggcatc tggagcgacg tcctgggtgt cgagcgggtc ggggttcacg acggcttctt
    12361 cgacctgggg gggcactcgc tgctcgccgc ccgcgtggtg tcgcgggtgc gcgaggcgct
    12421 gggggtggac ctgccgctgc gcacgatgtt cgacgcgccg caggtggccc gcttcgccgc
    12481 cacggtggag gcggcccggc aggccggcct tgcggaccgc ctgccgccgc tcgtcgcctc
    12541 gcaccacacg ggaccggcgc cgctgtcgtt cgcccaggag cgcctctggt tcctgcaaca
    12601 gctcgcgccc gagagcccgg cctatcacct ccctggagcg gtgcgtctgg aggggcggct
    12661 ggacatcccg gcgctcgccc gcgcgttgac cgaggtcgcg gcgcggcacg acatcctgtg
    12721 gacggccttc tcgccgtccc aggacggcga cagcgtggcg cagaccgtgc tccctccggg
    12781 ccccctgcct ctgcccgtgg tcgatctggg cctgctgccg gaggcggtca ggtgggacga
    12841 ggagcggcgg ctcgcccggc tggaggggcg gcgcctcttc gatctgagcc gcggcccgct
    12901 ggtgcgctcc gtcctgctgc ggctcggagc cgcagagcac gtgctgctgg tgaccctgca
    12961 ccacatcgcc gccgacggct ggtcgctggg gctgctggtg cgggagctca acgccctgta
    13021 cgcggcctcc ctcggcggct cggcgtcacc cctgccggcg ctgccggtgc gctacgagga
    13081 cttcgcccgc tggcagcggt cctggccggc cgccttcctg gcggcggagc tggcccactg
    13141 gacggcgcgc ctggaggggg cgccggcggt cctggcgctg ccggccgacc ggccgcgtcc
    13201 tggaacgcgg cgcgagcgag gggcgaagcg gccggtgcgg atcggcggcg agacggccgc
    13261 ggcggtccgg gtcctggcgc ggcggaccgc cgccagcccc ttcatggtac tcctcgccgc
    13321 tctcgacgcg ctgctgcagc ggctcaccgg ccagagcgac ctggtggtgg gctcgcccat
    13381 cgccgggcgc gaccgggtcg agctcgaggg gctgatcggc tgcttcgtca actccctggc
    13441 gctacgcgcg gaggcagacc cggagaggcc gttccgcgat ctggtcgccg gggtccgcca
    13501 ggctgccctc gacgcctacg ctcaccagga gctgcccttc gggcgcctgg tcgaggcgct
    13561 gcagccggag cgcaacctgg cctacacgcc gcttttccag gtgctgttcg tgctccagaa
    13621 cagtccgctg cccttcctcg accttcccgg actgcgtctg ggctggagcg aggtggagac
    13681 cggcacggcc aaattcgacc tcaccctctc cctcgaggag ggggaggacg gctggagcgg
    13741 cacgctggag tacgacgcgg acctgttcga cgccaccacg gcggagcgcc tggtggagcg
    13801 ctacgggctc ctgctcgcgg gcgccctcga ccggccggag gcgccgctgg cggagctgcc
    13861 ggcgctgacg gcggcggagc ggcaggcgct cctggtggag tggaacgaca ccgcggccct
    13921 ggaagacggc gccgacaccc tgccggccct cttcgccgcc caggcggcac gcacgcccga
    13981 cgccccggcc ctccgttgcg gcgccgaggt gctgagcttc cgcgagctgg accgccgcgc
    14041 cacccgtctg gcccgttgcc tacggcggat gggaatcggt cccgaggagc gggtcggcgt
    14101 gctgctgccg cggacgccac ggctggtcgt ggcgctgctg gcggtgctgc gggccggcgg
    14161 cgtctacgtg ccgctcgatc ccgcctaccc gcgccagcgg ctggccctca tgctggagga
    14221 cagcggcgcc cgcgggctgc ttaccgagcg cgccctgacg gagctcgccg gccaccgggt
    14281 ggattgggcg ctctgcctgg acgagatgcc ggaggagagt ctggaggaga tggcggacgc
    14341 cggggctccg gaagccggcg gcgaggtgga ggtgctgggc gatcacctgg cctatctcat
    14401 ctacacctcc ggctcgacgg gccggcccaa ggcggtcgcc atccgccacc gcagcgccgc
    14461 cgtcctgctg tgctgggcgc gggacacctt ccgggaagac gagcgccggg cgatcgcggc
    14521 ctcgacgtcg gtctgcttcg acctgtccgt cttcgagctg ttcacgccgc tggcctgggg
    14581 cgggaccgtg atcctgctcg gcaacgccct ggatctggcg gccctgccgg ccggctgtga
    14641 ggtcaccctg gtgaacaccg tgccgtcggc catgaccgag ctggtgcgcc tgggggccgt
    14701 gccctcctcg gtccgcaccg tcgcgttggc cggagagccg ctgccccgga cgctggccga
    14761 cagcctccac ggcctggggc acgtggggcg ggtggccaac ctctacggcc ccagcgagga
    14821 tacaacctac tccaccttct ctttcgtacc cacggaaggc ggcgctccgg ccatcggccg
    14881 ccccctggca ggcacgcgtg tttatctgct cgacgcctcg ggccggtcgg cgccgcccgg
    14941 ggtcgcgggc gagatctgcc tgggagggga ggggctggct cgcggctacc tggggcggcc
    15001 ggacctgacc gcggagaggt tcgtccccga tccggtgtcc ggcggcgggc ggctgtaccg
    15061 caccggcgat ctgggacgct atcgcgccga tggcgagatc gagttcctgg gtcggatcga
    15121 ccaccaggtc aaggtgcgcg gcttccgcat cgagctggga gaggtggaag aggcgctgct
    15181 gcggctgccc ggggtggcgg aggcggtggt ggtggtgcgc ggcgacgcga ccagcgaccg
    15241 gcggctggtg ggctatgtgg ggaccggctc cggcgccgcc cagccggaag agcggctgag
    15301 gagcctgctg ctggagagcc tgccggccta catggtgccg tcgtcgctgg tggtgctgga
    15361 ggcgctgccg cggacgccta acggcaaggt ggaccgcaag gcgctgcccg agccggaggc
    15421 gccgggagcc ggcgcggagc cggccggcgt cttcgtggcg ccgcgcaccg cgctggagga
    15481 gatcctggcg gggatcttcg ccgaggtgct gggagcggag cgggtgggtg tccgggacga
    15541 cttcttcgcg ctgggcggcc attcgctgct ggcagcgcgg gtggcatcgc gcctgcgcca
    15601 ggccttgggg gtggagctgc cgctgcgccg gctcttcggg agccccaccg tcgaggctct
    15661 ggccgcggcg gtggaggagc tgcgggggag cggcgaggcg tcctcgcagc cgctgccgcc
    15721 gctcgccacc gggccgcgga gtggcctgct gccgctgtcc tatgcccagg agcggctctg
    15781 gttcctggag cagctcgagc cgggctcggc ggcctacaac gttcccaccg cggtggacct
    15841 ggaggggcgg ctggacgttg tggcgctccg ccagagtctc gaggtcgtag tcgcccggca
    15901 cgagtcgctg cgcacggtct tcacggccat ggcgggcggt ccggctcagc gcgtcctgaa
    15961 gccgatgccg gtgccgctgc ccgaggtgga cctgacgggc ctgccgcatc cggagaggga
    16021 ggcccggcgt ctgggagccg aagaggccgc ccggccgttc gacctgaccc gcggccctct
    16081 gctgctgcga gcgcggctgc tgcgcctgga cgggcagcgc cggcgtctgc tgctgaccct
    16141 gcaccacatc gtctccgacg gctggtccat gggcgtgctg gtgcgggaga tcggggccct
    16201 ctacggccag ggggagcagg cgggcctgcc cgagctgccg gtgcagtacg cggactatgc
    16261 ggcctggcag cggagctggc tggaggggga gactctggag cgcgaggccg gcttctggcg
    16321 ccagcggctg gccggggctc cgccggtgct ggagctgccc accgaccggc cgcggccggc
    16381 ggtgtgtagc ggccgcggcg gcctgcgtca cctggagctc gcgccggagc tggtggacgg
    16441 agtcacgcgc ctggcgcggc gccagggggc gacgccgttc atggtgctgc tggccggctt
    16501 ctacgccctg ctgcatcgcg tcagcggggt ggaggacctg gtggtgggct cgccggtggc
    16561 tggccgggac cgctcggaga ccgagggtct gatcgggttc ttcgtgaaca ccctggtgct
    16621 gcgcgcggcg gtttccggcg aggtgtcggc cggtgagctg gtgcggcagg cgcgggagac
    16681 ggttctggag gcgcaggcgc accactggct gccgttcgag aagctggtgg agctgctgca
    16741 gccagcgcgc gacctgagct acacgccggt gttccaggtg atgctggcct accagaacga
    16801 tccgctgccg cctctcgacc tgccggagct gcggctgcgg ccggtggagg cggacggcgg
    16861 cacggccaag ttcgacctga cgctgtcgct gcgtcc
//
LOCUS       DASPJI010000594        10603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_670999, whole genome shotgun sequence.
ACCESSION   DASPJI010000594 DASPJI010000000
VERSION     DASPJI010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10603)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10603
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670999"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2596)
                     /locus_tag="VEL74_16595"
     CDS             complement(<1..2596)
                     /locus_tag="VEL74_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64197.1"
                     /translation="MEQNLQGFRLSPHQEHLWAFRMADPDAPYVALCEVEISGALDRA
                     RLAAALARVVERHEILRTSFRQVPGLVLPLQVIEGEAPVELPGELATTPWDPSQGAPL
                     RAALADLGGSRHSLLLALSALCADARTLSLLVAEIAREYAGGEGGGDQEGPIQYVDIA
                     EWHASLLEAEESLDGKKHWHRLGFLDGLDAALPFELRGHRGDGRFEPRTFPLVSDAGV
                     MERVEGLARQAGVPPATVLLGAWHVLLRRLTARTGLVVGLSVDGRKFEEMRDALGPCA
                     RHLPLLCETVDDEPFSGLVARLGETQAWVGEWQEYFDWREVPGAGGTPRSFPFCFEAG
                     DVAGPWTGAGVTFSIVRRHAFTEPFLLRLSVFRWQGGFAAEVQYDAGALAPEQAERLA
                     GWYRTLLEDALAHPGRPVGDLEMLSGTERAVCLVEWNGTATDFGPPTPLNELVWRQAE
                     RSPERIALVGDSGDSGNDGELSYAELRRRARLVAAYLRRLGVTPETPVALAVERSPEM
                     IVGLLGILEAGGAWVPMDPDYPADRLRWVREDSGARVLLTQERLAARLPAGTGEVVCL
                     DRDWPRIEALGAAPGGGGARPEGLAYVIYTSGSTGRPKGGMVEHRAIANRLLWIQSAF
                     PLTAGDRLLQKTPYSFDASIWEIFVPLLAGACVVLAEPGGQRDNAYLLDAIARHGVTT
                     LQLVPSQLRLFLEQDGLRAGCASLRRMFCGGEALPSVLAARLFERLEGSVSLCNLYGP
                     TESSIDATFHPWDPCDHLPELETVPLGRPIANVQVYLLDARWKPVPPGVPGELCIGGA
                     GLARGYRGRPDLTAERFVPHPESAVPGARLYRTGDLARHLEDGVIEFLGRIDYQVKVR
                     GFRIELGEI"
     gene            complement(2598..8099)
                     /locus_tag="VEL74_16600"
     CDS             complement(2598..8099)
                     /locus_tag="VEL74_16600"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64198.1"
                     /translation="MSGEEVESIYELSPAQQGMLFQSIYAPGSGVYVLQLSLRLSGKL
                     DLPLFERAWQRVVERHPILRTAFYWEDLEKPVQVVYRQVDLTLERESWRGLDAGEQSA
                     RLERYLVDDRERGFEPEEAPLMRLALFEVGQDLHHFVWSHHHLLNDGWSQGLLMRELF
                     TIYSALASGREPVLDRPRPYREYIVWLQRQDLGEAETFWRRYLAGLPAPTLVSGQDGQ
                     QAVATDFRSSRRRQLRLGADETAALREMARRHRLTLNTMVQGAWALLLARSLNVRQVV
                     FGAAVAGRPEDLPGAESIIGPFINTLPMRVDLAPESRLASWLAELQEGQVEQRRYAYA
                     PLYEVQQWSEVPAGLPLFDCILAFENYPLDSTLTSLLPGIGISEVRANELTNFPLELV
                     AVPGNELLLEMRHDLGRFEPARVARMLEHLAALLRSFARSSGAGLWELPALLPAERHQ
                     ILLEWNDTSRLHGLETSLHGLFERQAESAPDAVAAWFGEAGISYCELDRRAERLARHL
                     APLDRPDTQVYVALCMDRSLEMLIGLAGTLKAGLAYVPLEPAWPAERKRWILDSLQVP
                     CAVTGTSQLREMQELQWQLPALAELIVLDAGERRPPPEPLAVETVRELWDGVAERAGD
                     RVSAGGFVSSYTGRPFTEAEVDEYRDRVVSLASPWIRPDARVLEIGSGSGLILFEIAP
                     RVAHYVGLDPSGLTQERNREAVTVRGLANVALPTAFAHELDAAGTAGTAGTPGDGLFD
                     LVIIASTAQFFPGPLYLERVLSAAFQRLAPGGAVLVADVPDLRRREEFRASLEAFRAA
                     HRGEEGLRIRSRFDELYLDEDLFQDLAAELPDLAEAEVLHRRQGFDNELRFRYDVLLR
                     KAAPGEERGTRPARRKEIRTFWHLEAREDRALPHVSADAIAYVIFTSGSTGTPKGVVV
                     RHRPAVHLVDWVNRTFAVGPRDRVLFITSPCFDLSVYDVFGLLGAGGSLRIASGSDLR
                     DPERLVRWLIEDGITFWDSAPAALQQLVPFLPPTGAGGKALRLVFLSGDWIPLRLPDS
                     VRESFPEARVISLGGATEATIWSNFFLVRRVEPGWASIPYGRPIHDASYFVLDSWFEP
                     CPVGVAGDLYIGGECLASGYAGDPALTASKFLPDPWSLRPGGRLYRTGDLARFFSDGN
                     IEFLGRADHQVKVRGFRIELGEIEATLLRHPSVREAVVVALGTTLEDRRLAAYVVADP
                     VPAGDLKSWLQERLTDVMVPSTFTFLDAMPVTANGKLDRKALPEPGAASEPSEAGEGP
                     RTPLEERLCAIFSELLKIQRVHLRDSFFELGGHSLLATLLLARVRTELGVHLSLGQVF
                     REPTVAGLAALVLRLQREQEGAADSIVPGPPPDGPVPLSPGQRRLWFVEQLGSHGAYN
                     IAAAVRLVGPLDLGSLARTLAEVARRHETLRTRFEERSGQPVQVIEPPLVAEFVPELP
                     LVDLAVLPDGPRQEEARRLAAATARSPFHLGTAPLLRCLLLRLDAGEHAMVCSMHHII
                     SDGWSLRVLVRELAVLYRAFSRGLPSPLPEPPVQYRDFVLWEQRRLAGQVLDEQLDWW
                     RKHLAGAPAFLTLPADRPRPPVQSFRGSYRSFRLDAGLTEALRGVTAKEGCTLFMLLL
                     AAWQTLLHYLTGQDDIVVNAPLAFREQPEIEGAIGFFAQTLLFRIRFAGATTFSEVLG
                     RVRAGVEEAYLRPYAPLDRLVEELAPERNLSYTPFMQAGINYIDAEPLREPEEAGLKT
                     LPFEFDPGTSQFDLSLALSRATDGLEGGIQFRTDLFGAGTVTSFIHQLRHLLELVAAR
                     PEVPLQELGQAIASEAERLQAEANREMQQASRLTFQRRQRRGIDIASAN"
     gene            complement(8096..>10603)
                     /locus_tag="VEL74_16605"
     CDS             complement(8096..>10603)
                     /locus_tag="VEL74_16605"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HYG64199.1"
                     /translation="FVPDPFGTGAGLRLYRTGDLARHLPGGEIEFLGRLDFQVKIRGF
                     RIELGEIEAALLAHHQLREAVVLARAEESSQERRLVAYVVSDTGEEPEGLRRFLAQRL
                     PDYMVPPAIVMLPMLPLSANGKIDRAALPAPESAGAVAEHVPPRTALERFLASGFRDV
                     LALPPDRPVGLHDDFFALGGHSITGAIFINRLQEALSEIVHVVTLFDHPTVASLAAYV
                     AEQHPRAARRLWGEPAAVLETGAAARVGLAEIEEMRRLVVTGRPEVPEGLESEAPLNP
                     PAVFVLSPPRSGSTLLRVMLGGHPELFAPPELELLSFPTLTQRRQAFQGRDSFWLEGV
                     VRAVMEARGCGAEEAERLMTEREAEGWSTRRFYGELQGWLGERLLVDKTPSYALDPEV
                     LVRAESWFAGPRYLHLMRDPRATNLSFEEAKLDQIFFRRPHGFSRRQLAELFWTVCHR
                     NILDFLAALPRERWLTVRFEDLVREPERVLSGICEFLGIDYRPEMADPYREGRRSRMT
                     DGVHDVSRMLGDVKFHTHSRVDSSAAERWRQAEEVPLGAPARELALELGYVDTAAEPV
                     RTDVLVQLQAGAPEVGPLFCVHPVGGDVACYRELARRLGSDQPVYGLRSPQEPVTDLR
                     QMAALYVEAARQAQPTGPYRLAGWSMGGLVAYEMARQLVAQGEAVELVALIDAASPQW
                     WAEQPRPSDAELVAGFSRDLVRLFGAAQVPAVRLDAADVGGNLARVLEVGHRAGVVPE
                     SVGLSDLRRLFDIFRANRLALSSYPSLPYSGSLELIRATASMQEVGEDLYQGWSELAT
                     GGVTVHDLQGDHYSIVLGDGVEQLAASLRDAFKRVTK"
BASE COUNT         1717 a         3814 c         3710 g         1362 t
ORIGIN      
        1 cgatctctcc cagctcgata cggaatcccc ggaccttgac ctggtaatcg atgcgcccca
       61 ggaactcgat caccccgtct tcgaggtgcc gggccaggtc gccggtgcga tagagcctgg
      121 cgccgggaac ggcgctctca gggtgcggca cgaagcgctc cgccgtcagg tcgggccgtc
      181 ctctatagcc ccgcgccagg ccggcgccgc cgatgcacag ctcacccggc acaccgggag
      241 ggacgggctt ccagcgcgcg tcgaggagat agacctggac gttggcgatg ggacgcccca
      301 gggggacggt ctccagttcc ggcaggtgat cgcacgggtc ccacgggtga aaggtggcgt
      361 cgatggagga ctcggtcggc ccgtagaggt tgcagaggct gacagagccc tcgagccgct
      421 cgaagaggcg tgccgccaga acggacggca gggcctcgcc gccgcagaac atgcggcgca
      481 aggaggcgca gccggcgcgc agcccgtcct gctccaggaa gagcctgagc tgggaaggaa
      541 cgagctgcaa ggtcgtcacc ccgtgcctgg cgatggcgtc gaggaggtag gcgttgtcgc
      601 gctgcccacc cggctcggcc agcaccacgc aggcgccggc cagcaacggc acgaagatct
      661 cccagatgga ggcgtcgaag ctgtagggcg tcttctgcag caggcggtcg cccgccgtga
      721 gaggaaaggc gctctggatc cagagcagcc ggttggcgat ggcgcggtgc tcgaccatcc
      781 cccccttggg gcggcctgtg gagccggagg tatagatcac gtacgctagc ccctcgggcc
      841 gcgctccacc accgcccggg gcagccccca gcgcctcgat ccgcggccag tcccggtcca
      901 ggcagacgac ctcgccggtg cccgcgggca gacgggcggc gagccgctcc tgggtgagca
      961 ggaccctcgc tccggaatcc tcccggaccc agcgcagccg gtccgccggg tagtcgggat
     1021 ccatgggcac ccacgcgccg ccggcttcga ggatgcccag cagtccaacg atcatctcgg
     1081 gcgagcgctc gacggcgagg gccacgggcg tctccggcgt gacgcccaga cgccgcaggt
     1141 aggcggccac aagccgggcg cgacgccgca gctccgcgta gctcagctcg ccgtcgtttc
     1201 cgctgtctcc gctatctccg acaagggcga tgcgctccgg gctgcgctcc gcctgccgcc
     1261 agaccagctc gttgagaggc gtcggcgggc cgaagtcggt cgccgtgccg ttccactcca
     1321 cgaggcagac ggcgcgctcg gtgccactca gcatctcgag gtcgcccacc ggccggccgg
     1381 gatgcgcgag ggcgtcttcg agcagcgtcc tgtaccaccc ggccaagcgc tccgcctgct
     1441 ccggcgccag agctcccgcg tcgtactgga cctcggcggc gaaaccgccc tgccagcgga
     1501 agacggagag acgaaggagg aaaggctcgg tgaaggcgtg ccgccggacg atggagaagg
     1561 tcacgcccgc gccggtccac ggacccgcga cgtcgccggc ctcgaagcag aacgggaagg
     1621 agcgcggcgt cccaccagcc cccggcacct cccgccaatc gaagtactcc tgccactcgc
     1681 ccacccaggc ctgggtttcg ccgagccggg caaccaggcc gctgaagggc tcatcgtcca
     1741 cggtctcgca gaggagcggc aggtggcggg cgcaaggccc cagggcgtcc cgcatctcct
     1801 cgaacttgcg gccatccacg gagagcccga cgaccaggcc ggtgcgcgcc gtgagccggc
     1861 gcaggaggac gtgccaggca ccgagcagga cggtggccgg cgggacgccg gcttggcggg
     1921 cgagcccctc gacccgctcc atcactccgg cgtccgagac cagcgggaag gtccgcggct
     1981 cgaaccggcc gtcccctcgg tggcccctga gctcgaacgg gagagcggcg tcgagcccgt
     2041 cgagaaagcc caggcgatgc cagtgcttct tgccgtccag ggactcctcg gcttcgagca
     2101 gagacgcgtg ccactcggcg atgtcgacgt actggatcgg cccctcctga tccccgcccc
     2161 cctctccacc ggcgtactcg cgggcgatct cggccaccag gaggctgagc gtcctggcat
     2221 ccgcgcagag agccgacagc gccagcagga gcgaatgacg ggaaccgccg aggtccgcca
     2281 gggccgcgcg caggggcgcc ccttgcgagg ggtcccaggg ggtcgtcgcc agctctccgg
     2341 ggagctcgac gggagcctca ccttcgatga cctggagcgg cagcacgagc cccggcacct
     2401 gacggaagga ggtgcggagg atctcgtgcc gctccaccac gcgggccagc gcggccgcga
     2461 gcctcgcccg atcgagagcg ccggagatct ccacctcgca gagcgccaca tagggcgcgt
     2521 ccgggtccgc catgcggaac gcccagaggt gctcctggtg aggggagaga cggaatcctt
     2581 ggaggttctg ttccatatca gttggcgctc gcgatatcga tgccgcggcg ttggcggcgc
     2641 tggaaggtga ggcgtgacgc ctgctgcatc tcccggttcg cttcggcctg cagccgttcc
     2701 gcttcgctgg cgatggcctg ccccagctcc tgtagcggga cctccggccg ggcggcgacc
     2761 agctccagca gatggcgaag ttggtggatg aacgaagtga ccgtcccggc gccgaacaga
     2821 tcggtccgga actggatccc cccttcgagc ccgtccgtgg cacggctcag ggccagcgac
     2881 agatcgaact gggaggtacc ggggtcgaac tcgaaaggca gcgtcttgag gccggcctct
     2941 tccggctccc gcagcggctc ggcgtcgatg tagttgatcc cggcctgcat gaacggcgtg
     3001 tagctcaagt tccgctccgg cgccagctcc tccaccaggc ggtccagagg ggcatagggg
     3061 cgcaggtagg cctcttcgac cccggcccgg acccggccga gcacctcgga gaaggtcgtc
     3121 gcgccggcga agcggatccg gaagagcagc gtctgggcga agaagccgat ggccccctcg
     3181 atctccggct gctcgcggaa ggccaggggg gcgttgacca cgatatcgtc ctggcccgtg
     3241 aggtagtgca ggagggtctg ccacgcggcc aggaggagca tgaacagggt gcaaccctcc
     3301 ttcgcggtca ccccgcgcaa cgcctccgtc agcccggcgt ccagccggaa gctccggtag
     3361 gagccgcgga agctctgcac cggcgggcgc ggccggtccg cgggcaaggt caggaaggcc
     3421 ggggctccgg ccaggtgctt ccgccaccaa tcgagctgct cgtcgagcac ttgccccgcg
     3481 aggcgccgct gctcccagag cacgaagtcg cggtactgga ccggcggctc cgggagcggc
     3541 gacggcagcc cccgggagaa ggcccggtac aggaccgcca gctcccgaac cagcacgcgc
     3601 agcgaccagc cgtcggagat gatgtggtgc atggagcaga ccatcgcgtg ctctccggcg
     3661 tccaggcgca gcaacaggca gcgcaggagc ggcgcagtgc ccagatggaa ggggctccgg
     3721 gcggtggccg ccgcgagccg gcgcgcctcc tcctgacggg ggccgtccgg caggacggcc
     3781 agatcgacca gcggcagctc cgggacgaat tccgcgacga gcgggggctc gatcacctgg
     3841 acgggctggc cgctccgctc ctcgaaccgg gtccgcaagg tctcgtggcg ccgcgccacc
     3901 tccgccagcg tccgagccag ggacccgagg tccagaggac ccacgaggcg gacggcggcg
     3961 gcgatgttgt aggcgccgtg ggaaccgagc tgctcgacga accagagccg ccgctgtccg
     4021 ggtgacagcg gcacgggacc gtccggcggg gggccgggca cgatgctgtc ggccgcgccc
     4081 tcctgctccc tctgcagccg cagcaccagc gccgcgagcc cggccacggt cggctcgcgg
     4141 aacacctgcc cgagggagag atggaccccg agctcggtcc gcacacgggc gaggagcagg
     4201 gtggcgagga gcgaatgccc tcccagctcg aagaagctgt cgcggagatg cacccgctgg
     4261 atcttgagca gctcggagaa gatcgcgcag aggcgctcct ccaggggtgt ccggggacct
     4321 tcgccggcct ccgagggttc ggacgccgcg cccggctcgg gcaacgcctt ccggtcgagc
     4381 ttgccgttgg cggtgaccgg catggcgtcg aggaaggtga aggtcgacgg caccatgaca
     4441 tccgtcagcc gctcctgcag ccaggacttg agatccccgg caggtaccgg gtcggccacc
     4501 acataggctg cgaggcggcg gtcctcgagc gtcgttccaa gagctaccac cacggcctcg
     4561 cggaccgagg gatggcgcag cagcgtcgct tcgatctccc ccagctcgat gcggaagccg
     4621 cggaccttga cctggtggtc ggcgcgcccc aggaactcga tgttcccgtc ggagaagaag
     4681 cgcgccaggt caccggtgcg atagagccgg ccgcccgggc ggaggctcca gggatcgggc
     4741 aggaacttgg aagcggtcag cgccgggtcg ccggcgtagc cggaggccag gcattcgccg
     4801 ccgatgtaca gatcgccggc gacgcccact gggcagggct cgaaccagga atcgagcacg
     4861 aagtagctgg cgtcgtggat cggccgtcca taggggatgc tcgcccaccc gggctcgacc
     4921 ctgcggacga ggaagaagtt cgaccagatc gtcgcctcgg tggcgccgcc gaggctgatc
     4981 acccgggctt ccgggaagct ctcccgcacg ctgtccggca ggcggagggg gatccaatcg
     5041 ccgctcagga acaccagacg cagcgccttg ccgcctgcgc ccgtgggcgg caggaagggg
     5101 acgagctgct gcaaggccgc cggggccgag tcccagaagg tgatgccgtc ctcgatcagc
     5161 catcgcacca gccgctccgg gtctctgagg tcggagcccg acgcgatccg tagcgagccg
     5221 ccggccccga gcaggccgaa gacgtcatag accgagaggt cgaagcaggg ggaggtgatg
     5281 aacaggaccc ggtcgcgcgg cccgaccgcg aaggtgcggt tgacccagtc caccaggtgg
     5341 acggcgggcc ggtggcgcac cacgaccccc ttgggagtgc cggtggagcc cgaggtgaag
     5401 atcacgtagg cgatggcatc ggcggagaca tggggcaaag cccgatcctc ccgggcttcc
     5461 aggtgccaga acgtgcggat ctccttgcgg cgcgccggcc gggtccccct ctcctctcca
     5521 ggagccgcct tgcgcagcag cacgtcgtag cggaagcgca gctcgttgtc gaacccctgg
     5581 cggcggtgga ggacctcggc ttcagccaga tcgggcagct cggccgccag atcctggaag
     5641 agatcctcgt ccaggtacag ctcgtcgaac cgggagcgga tccgcaaccc ctcctcgccg
     5701 cgatgggcgg cccggaacgc ttcgagagag gcccggaact cctcgcggcg ccgcaggtcc
     5761 ggcacatcgg cgaccagcac ggcccctccc ggagcgaggc gctggaaggc cgccgacagg
     5821 acccgctcga ggtagagcgg gccggggaag aactgcgccg tgctggcgat gatcacgagg
     5881 tcgaacaggc cgtccccggg ggtgccggca gtgccggcgg tgccggcggc atcgagctcg
     5941 tgggcgaaag cggtagggag cgccacgttg gcgagcccgc gcacggtgac ggcttcgcgg
     6001 ttgcgctcct gcgtcagccc ggaaggatcg aggccgacgt agtgggcgac acggggggcg
     6061 atctcgaaga ggatcaggcc ggagccgctg ccgatctcca gcacccgggc atccggccgt
     6121 atccaggggg aagccagcga gaccacccgg tcccggtact cgtccacctc ggcctcggtg
     6181 aacggccggc cggtgtagct gctgacgaag cctccggcgc tcacgcggtc gccggcccgc
     6241 tccgcgactc catcccagag ctctctcacc gtctcgacgg ccaagggctc cgggggcggg
     6301 cggcgctctc ccgcgtcgag gacgatcagc tcggcgaggg cggggagctg ccactgcagc
     6361 tcctgcatct cccggagctg cgaagtgccg gtcacggcac aggggacttg cagcgagtcg
     6421 aggatccagc gcttgcgctc cgccggccag gcgggctcga ggggtacata ggccaggccg
     6481 gccttgagcg tgccggcgag gccgatcagc atttcgagcg accggtccat gcagagcgcg
     6541 acgtagacct gtgtatccgg cctgtccagg ggtgcaaggt gccgggccag gcgctccgcc
     6601 cggcggtcga gctcgcagta gctgattccg gcctccccga accaggcggc gaccgcatcg
     6661 ggggcgctct ccgcctgccg ctcgaacagg ccgtggaggg aggtctccag accatgcagg
     6721 cggctcgtgt cgttccactc cagcaggatc tggtgccgct cggccggcag cagcgccggc
     6781 agctcccaca gaccggcccc tgagctccgc gcgaagctcc gcagcagagc cgcgaggtgc
     6841 tcgagcatgc gcgccaccct ggcgggctcg aagcggccga ggtcgtggcg catctcgagc
     6901 aacagctcgt tgccggggac cgccaccagc tccaggggga agttcgtcag ctcgttcgcc
     6961 cggacctcgg agatcccgat accgggcagc agcgaggtga gcgtggagtc gagcggatag
     7021 ttctcgaagg ccaggatgca atcgaagagg gggaggcccg ccggcacctc gctccactgc
     7081 tgcacctcgt agagcggcgc gtaggcataa cggcgctgct ccacctggcc ctcctgcagc
     7141 tccgccagcc aggaggccag acggctctcc ggcgccaggt ccacccgcat gggcaaggta
     7201 ttgatgaacg ggccgatgat cgactccgct cccgggaggt cttccggccg gccggcgacc
     7261 gctgccccga agaccacctg ccgcacgttc agcgagcggg ccagcaggag cgcccaggcc
     7321 ccctgcacca tggtgttgag cgtcaggcga tggcgccgcg ccatctcgcg cagcgccgcg
     7381 gtctcgtccg cccccagccg cagttgccgg cgccgggagc tgcggaagtc ggtcgccacc
     7441 gcctgctgcc cgtcctggcc ggagaccagc gtgggggccg ggagccccgc gaggtaccgg
     7501 cgccagaagg tctccgcctc gccgaggtcc tggcgctgga gccagacgat gtactcgcgg
     7561 tacggccgcg gccggtcgag aacgggctcg cggccgctgg ccagcgccga atagatggtg
     7621 aacagctccc gcatcaggag cccctgagac cacccatcgt tgagcaggtg atggtggctc
     7681 cagacgaagt ggtggagatc ctggccgacc tcgaagagcg ccaggcgcat caggggcgcc
     7741 tcctccggct cgaacccacg ctcccggtcg tcgaccaggt agcgctcgag ccgggcgctc
     7801 tgctcgccgg catcgaggcc gcgccaggac tcacgctcca gcgtcagatc cacctggcga
     7861 tagaccacct gcacgggctt ctcgagatcc tcccagtaga aggcggtgcg caggatgggg
     7921 tgccgctcga cgaccctctg ccaggcccgc tcgaacagag ggaggtcgag cttgccggac
     7981 aggcgcaggc tcagttggag gacgtagacg cccgagccgg gggcatagat gctctggaag
     8041 agcatgccct gctgcgcggg agagagctcg tagatggact cgacttcttc gccgctcatt
     8101 tcgtcaccct cttgaacgcg tctctcaggg aggcagccaa ctgctccacc ccatccccca
     8161 gcacgatcga gtagtgatcg ccctggaggt cgtgaacggt gactccaccg gtcgccagct
     8221 cgctccagcc ctggtaaagg tcctccccta cctcctgcat cgacgccgtg gcgcggatca
     8281 gctccagaga tccggagtaa ggcagcgacg gataggagga gagcgccagg cggttcgccc
     8341 ggaagatatc gaacagccgg cgcaggtccg aaaggccgac gctctccggc accacacccg
     8401 cgcggtgacc gacttccagc acccgcgcca agttgccgcc aacgtccgcg gcgtcgagcc
     8461 gcaccgccgg tacctgcgcc gccccgaaca gccgcaccag gtcacgcgag aacccggcca
     8521 ccagctccgc gtcgctcggc cggggctgct cggcccacca ctgcggcgac gcggcgtcga
     8581 tcagcgccac cagctccacg gcctcaccct gcgccacgag ctggcgcgcc atctcgtagg
     8641 ccaccagacc gcccatcgac catcccgcca gccggtaggg ccctgtcggc tgcgcctggc
     8701 gcgccgcctc gacgtacagc gccgccatct gccgcagatc ggtgaccggc tcctgaggcg
     8761 agcgcagacc atacacaggt tgatccgaac ccaagcgccg cgccagctcg cggtagcaag
     8821 ccacgtcgcc gcccaccgga tgcacgcaga acagcggccc gacctccggc gcgcccgcct
     8881 ggagctggac cagaacgtcg gtccggaccg gctccgccgc ggtatccaca tagccgagct
     8941 ccagggccag ctcccgtgcc ggggcgccca gcggcacctc ttccgcctgg cgccagcgct
     9001 ccgccgccga cgagtccacc cgcgagtggg tgtggaactt gacatccccc agcatgcgcg
     9061 acacgtcgtg gacgccatcg gtcatgcgcg accggcgccc ttcacggtag ggatctgcca
     9121 tctccggccg gtagtcgatc cccaggaact cgcagatgcc cgacagcacc cgctccggct
     9181 cgcgcaccag atcctcgaac cgcaccgtca gccagcgctc ccgcggcagc gccgccagga
     9241 agtccaggat gttcctgtgg cacaccgtcc agaacagctc cgccaactga cggcggctga
     9301 acccgtgcgg ccgacggaag aagatctggt ccagcttggc ctcttcgaac gacaggttcg
     9361 tcgcccgcgg atcgcgcatt aggtgcaggt agcgcgggcc ggcgaaccag gactcggccc
     9421 gtaccaaaac ctccgggtcc aacgcatagg agggcgtctt gtccaccagc aggcgctcgc
     9481 ccagccagcc ctgcagctcg ccgtagaagc gccgggtcga ccagccttcg gcctcgcgct
     9541 cggtcatgag ccgctcggcc tcttcggcgc cgcagccgcg ggcctccatc accgcccgca
     9601 ccaccccttc gagccagaag ctgtcgcgcc cctggaaagc ctggcgccgt tgcgtcagag
     9661 tggggaacga cagcagctcc agctccggcg gcgcgaagag ttccgggtga ccgcccaaca
     9721 tgacccgcag cagggtcgat cccgagcgcg gcggcgacag cacgaacacc gccggcggat
     9781 tgagcggcgc ctcgctctcc agcccttccg gcacctccgg acggccggtg acgaccagcc
     9841 ggcgcatctc ctcgatctcg gctagaccca cccgcgccgc cgcgccggtc tccaggaccg
     9901 ccgccggctc gccccagagc cgccgcgccg cccgcgggtg ctgctcggcc acataggcgg
     9961 ccagtgacgc caccgtcggg tggtcgaaca gggtcaccac gtgcacgatc tccgacaagg
    10021 cctcttgcag gcggttgatg aagatggcgc cggtgatgga gtggccgccc agggcgaaga
    10081 agtcgtcgtg caggccgacc ggacgatccg gcggcagcgc cagcacgtcg cggaacccgg
    10141 aggccaggaa ccgctccaga gccgtacgtg gaggtacgtg ctccgcaacg gcgccggctg
    10201 attctggcgc cggcagcgcc gcccggtcga tcttgccgtt ggccgacagc ggcagcatcg
    10261 gcagcatgac gatcgccggc ggcaccatgt agtccggcag ccgctgggcc aggaagcggc
    10321 gcaggccctc cggctcctct ccggtatcgg acaccacgta ggccaccagc cgccgctcct
    10381 ggctactctc ttcggcgcgg gccagcacca cggcctcgcg gagctggtgg tgagccagca
    10441 gcgccgcttc gatctccccc agctcgatac ggaagccgcg gatcttgacc tggaagtcga
    10501 gccggcccag gaactcgatc tcgccccccg gcaggtgccg cgccaggtcc cccgtccggt
    10561 acagccgcaa gcccgctccg gtcccgaacg gatcgggcac gaa
//
LOCUS       DASPJI010000595         5046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_264911, whole genome shotgun sequence.
ACCESSION   DASPJI010000595 DASPJI010000000
VERSION     DASPJI010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264911"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VEL74_16610"
     CDS             complement(<1..412)
                     /locus_tag="VEL74_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HYG64200.1"
                     /translation="MRTYASLLDLMKDSPLVAIPVRDGTRDRARLWAQLELALPGAMK
                     DRPALQIIEEAEAAGILKPGSVIVESSSGTMAEGLARAGVLKGYRVIIVTDPRIDAMT
                     SAKLRSLGAELEVVDTYAPQGGWQLSRLRRLREVL"
     gene            complement(430..1986)
                     /locus_tag="VEL74_16615"
     CDS             complement(430..1986)
                     /locus_tag="VEL74_16615"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HYG64201.1"
                     /translation="MSVDKMGRLSLPPDPTLFELLYEPGLDEDRRTVLPYLLRVDAAH
                     ILMLARQEILPRATAAKLLTVNRDLAGRCTAGETLFVARESHRGLYWLYEQEYIRQLG
                     GIVGGAAHVARSRNDINATVTRLRMRDEALALLAACDALLAALHSQAEKHAATLMSFF
                     THFQPAQPSTLGHYLTGVFGELLRTAEWLLAAFPALDRSPMGAAAGAGTSFPLDREEV
                     ARLLGLAEVIENSADAVASRDYAVQLLSGAALLGTTLTRLSQDLQSWGSFAYGFLDWP
                     DDLVSTSSIMPQKRNAFVLESIRGQAIQATGALTATLAGLKNTPFTNGVEVSSGATVH
                     AWPAFQASYKAVRLTTLLVERLEARPERMRAFLAGAQTTMTAVADLLVARHGLPFRTA
                     HDAVGALLQELPADAELSPGIVKAGLEPVIAAATGDSVELDLSELEAALDPWRCLLAA
                     RHGGGPAPESVRAQLRSLAERHQHLRHRIASRRRSRDEADALLAREIASFLAVADGPL
                     PDPSGEERRA"
     gene            2134..2898
                     /locus_tag="VEL74_16620"
     CDS             2134..2898
                     /locus_tag="VEL74_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG64202.1"
                     /translation="MKIDKAEAIVTGAARGLGRYFALALAREGARVVAADINPAGLRS
                     LKAEAAGLPGSLTVAAVDITNESEVVSFVDAAARELTGLNILLNNAGILRDGALVAPE
                     QESVRRLPLMLWSKVVDVNLTGQFLMAREVASKMLERKAQGGVIVNISSLARAGNVGQ
                     SSYSASKAGLDACTRTWALELAHYGIRVGGVAPGVIDTPILEEISEEALEALVHGIPL
                     GRIGTPDEVWQAVRFILVCEFFTGRTIEVDGGARMG"
     gene            complement(2912..4102)
                     /gene="aroC"
                     /locus_tag="VEL74_16625"
     CDS             complement(2912..4102)
                     /gene="aroC"
                     /locus_tag="VEL74_16625"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HYG64203.1"
                     /translation="MRRLRLTTGGESHGPGLTALLTGLPAGLRVDLGLLARDLARRQH
                     GYGRGRRMEIEKDTAEIRGGVRGGETLGSPVVLWIANRDYANWEAVMGPEQVDPRLAE
                     LRRLKSPRPGHADLPGGLKYLRRDLRDVLERASARETTARVAAGAFARMLLAEFGVEI
                     RSGVRSLGPIGAGAPVPTWEDLLQVDDASPLRAIDRSLEEEMVRLVEQAKADGDTLGG
                     AVTVIAHNVPAGLGSHVQWAEKLDGRLAQAVMSVHAVKAVEIGAGVAASQGFGSAAHD
                     AIGREGDRWVRPTNRAGGLEGGITNGEDLVITAYKKPIATLRQGMPSVDLDTLEPHGS
                     QYERSDVTALPAAGVIAEAMVALVLADALLEKLGGDSMAEIKAHWEATLAQQRAWPGT
                     SQGV"
     gene            4182..>5046
                     /locus_tag="VEL74_16630"
     CDS             4182..>5046
                     /locus_tag="VEL74_16630"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HYG64204.1"
                     /translation="MTDTTNTWQPILEGDLADRARGAVEEIAESLKPAILAADPSQDV
                     SALAPWRRSTFSLAGGRSGEALLFTYLDQAFPGRGYDGVAALALEQASEDLGQVQAPP
                     GLYSGFSGVAWTVEHLQGRLIEPDDDPGEDIADAMAEHLSITPWRGDYDLISGLVGYG
                     VYALERMPRPQGEECLARVVARLDETAEKGADYVTWLTRPELLPPRTREETPEGNYNA
                     GVAHGVPGVIGLLGEVCAAGVALEQARPLLEGSVSWLLDHRLPPGGFSVFPYSVIPGR
                     ELSSTRLAWCYG"
BASE COUNT          729 a         1699 c         1852 g          766 t
ORIGIN      
        1 cgagcacctc gcgcagccgg cgcagacggg agagctgcca gccgccctgc ggcgcgtagg
       61 tgtcgaccac ctcgagctcc gcccccagcg agcgtagctt cgccgaggtc atggcgtcga
      121 tccgcggatc ggtgacgatg atgacccgat agcccttgag cactccggcg cgcgccaaac
      181 cttcagccat ggtgccggag gagctttcga cgatcacact acccggcttc aagatgcccg
      241 cggcctccgc ctcttcgatg atctgcagcg cgggccggtc cttcatggca cccggcaggg
      301 cgagctcgag ctgcgcccag agccgtgcgc gatcccgggt cccgtcccgc acggggatcg
      361 ccaccagggg agagtccttc atcaggtcca agagggatgc gtacgttctc atgctcttcc
      421 tcctcttcct catgctcttc tttcttcgcc gctcggatcc gggagcggac cgtccgccac
      481 ggcgaggaac gatgcgatct cccgggcgag aagggcatcg gcctcgtccc gcgacctccg
      541 ccgggaggcg atccggtgtc gcagatgttg gtgccgctcg gcgagggacc tgagctgtgc
      601 gcggaccgac tcgggggccg gtccgccgcc gtggcgcgcc gccagcagac agcgccaggg
      661 gtcgagagcg gcctccagct cggagagatc gagctccacg gaatcgccgg tagccgccgc
      721 gatgaccggc tcgagcccgg ccttgacgat cccggggctc agctcggcgt ccgccgggag
      781 ctcctggagc agggcgccga ccgcatcgtg ggcggtacgg aaggggagac cgtggcgggc
      841 cacgaggaga tcggcgacgg ccgtcatcgt cgtctgagcc cctgccagga aggcgcgcat
      901 ccgctccggg cgcgcctcca gccgttcgac gagcagggtc gtcagccgca cggccttgta
      961 gctggcctgg aaggccggcc aggcgtggac ggtcgccccg gagctcacct cgacgccgtt
     1021 ggtgaaggga gtgttcttga ggccggccag ggtggcggtc agagcgccgg tcgcctggat
     1081 cgcctggccg cggatgcttt cgaggacgaa ggcgttgcgc ttctgaggca tgatcgagct
     1141 ggtgctcacg aggtcgtccg gccagtcgag gaagccgtag gcgaagctcc cccagctctg
     1201 caggtcctgc gacaggcggg tgagcgtcgt accgagcaga gcggcgccgc tgagaagctg
     1261 gacggcatag tcgcgcgagg cgaccgcgtc ggccgagttc tcgatcacct cggcgagacc
     1321 gaggaggcgc gcgacctcct cccggtcgag tgggaacgag gtgccggcgc cggcggcggc
     1381 ccccatgggc gagcggtcga gggcggggaa ggcagccagg agccattcgg ccgtccgcag
     1441 gagctcgccg aagactccgg tcagatagtg cccgagggtg ctcggctgcg ccggctggaa
     1501 atgggtgaag aagctcatca gagtggccgc gtgcttctcg gcctggctat ggagagcggc
     1561 gagcagagcg tcgcaggcgg cgaggagcgc cagggcctcg tcgcgcatcc gcagccgggt
     1621 cacggtggcg ttgatgtcgt tgcggctgcg ggcgacgtgg gcggcgccgc ccacgatacc
     1681 tccaagctgc cggatgtact cctgctcgta gagccagtaa aggccccggt ggctttcccg
     1741 cgcgacgaaa agggtctcgc cggcggtaca tcgcccggcg aggtcccggt tcaccgtcag
     1801 cagcttcgcc gcggtcgcgc gcggcaggat ttcctgccgc gcgagcatga ggatgtgggc
     1861 ggcgtcgacg cgcagcaggt aggggaggac cgtccgccgg tcctcgtcga gacccggctc
     1921 gtacagcagc tcgaacaggg taggatccgg tggcagagag agccggccca tcttgtcaac
     1981 actcacattg gctccccatc ggcggcattc attcgaattc tgcgcatact aacattggtg
     2041 cgccaaggtg gcgccttgga tcttgccggc cggaggcagg cggtgattca atcctctagg
     2101 tccagtcagc acatgggccg ctgggagcga gccatgaaga tcgacaaagc ggaagcgatt
     2161 gtaacggggg ccgcccgggg actcgggcgc tactttgcct tggcgttggc gcgcgaaggg
     2221 gcgcgggtgg tcgccgccga tatcaacccg gcagggctca gaagcctcaa agcggaggcg
     2281 gcaggtctgc ccggctccct gaccgttgcc gcggtcgaca tcacgaacga gagcgaggtc
     2341 gtttctttcg tcgatgccgc ggcgcgggag ttgaccgggc tcaacatctt gctcaataac
     2401 gcgggcatcc ttcgcgacgg tgctctagtg gcccccgagc aggagagcgt ccggcgcctg
     2461 cctctcatgc tgtggagcaa ggtggttgac gtgaacctga cagggcagtt cttgatggcc
     2521 cgcgaggtgg cgtcgaagat gctggaacgg aaggcccagg gcggagtcat cgtcaacatc
     2581 tcgtccctgg cacgcgccgg taacgttgga cagtccagct actccgcttc caaggcgggg
     2641 ctcgatgcat gcacccgcac ctgggcgctg gaactggctc attatgggat ccgggtcggg
     2701 ggagtggccc ccggagtgat cgacacgccg atcctcgagg aaatctcgga ggaagcgctc
     2761 gaagcgctcg tgcatggaat cccactggga cgaattggca ctcccgacga ggtctggcag
     2821 gcagtacggt tcatcctcgt gtgtgagttc ttcaccggcc gcacgatcga agtcgacggc
     2881 ggagctcgca tgggttgacc tctatggcct gctaaacacc ctgggacgtc ccgggccacg
     2941 cccgctgctg ggcgagcgtg gcctcccagt gggccttgat ctccgccatc gaatcccctc
     3001 ccagcttctc gagcagcgcg tcggccagca ccagggcgac catggcctcg gcgatgaccc
     3061 cggcggcggg gagggcggtg acgtcgctcc gctcgtactg cgagccgtgg ggctccaggg
     3121 tgtcgaggtc gacgctgggc attccctggc gcagggtggc gatgggcttc ttgtaggcgg
     3181 tgatcaccag gtcctcgccg ttggtgatcc ccccttcgag gccgcccgcc cggttggtgg
     3241 ggcggaccca gcggtccccc tcccgcccaa tcgcgtcgtg cgccgcgctg ccgaagccct
     3301 ggcttgccgc gacgcccgcg ccgatctcca ccgccttgac cgcgtgcacc gacatcaccg
     3361 cctgggcgag ccggccgtcc agcttctcgg cccactggac gtgggagccg aggcccgcgg
     3421 gcacgttgtg ggcgatcacc gtgaccgcgc cgcccaacgt gtcgccgtcc gccttggcct
     3481 gctcgaccag ccgcaccatc tcttcttcga gcgagcggtc gatggcgcgc aggggggagg
     3541 cgtcgtcgac ctggaggagg tcctcccagg tggggaccgg ggcgccggcg ccgatcgggc
     3601 cgagggagcg cacgccgctg cggatctcga cgccgaactc ggcaaggagc atgcgggcga
     3661 aggcgccggc cgccacccgg gcggtggtct cgcgggccga cgcccgctcc agcacgtcgc
     3721 gcaggtcgcg ccgcaggtac ttgaggccgc cggggaggtc ggcgtgcccc ggccgcgggg
     3781 acttgagccg ccggagctcg gcgagacgcg ggtccacctg ctcgggaccc atgaccgcct
     3841 cccagttggc gtagtcgcgg ttggcgatcc agaggacgac gggggagccc agggtctcgc
     3901 cgccgcggac gccgccgcgg atctccgccg tgtccttctc gatctccatc cgccgtcccc
     3961 ggccgtagcc gtgctggcgg cgggcgaggt cgcgggcgag caggccgagg tccacccgca
     4021 ggccggcggg caggccggtg aggagggcgg tcagccccgg cccgtggctc tcccctcccg
     4081 tggtcaggcg cagacggcgc atggcggcga ggatatcagg agcggaggag ggagccgggg
     4141 tggggtatga tcccgccctg acttttgcga gagatctcga cgtgaccgat acgacgaaca
     4201 cctggcagcc cattctcgaa ggggatctcg ccgaccgcgc gcgcggcgcg gtcgaggaga
     4261 tcgccgaatc gttgaagccc gcgatcctgg cggcggaccc gtcgcaggac gtctccgcgt
     4321 tggcgccctg gcggcggtcc accttctcgc tggccggcgg ccggtcgggc gaggctctcc
     4381 tcttcaccta tctcgaccag gcgttcccgg gccggggcta cgacggcgtc gccgccttgg
     4441 cgctggagca ggcgagcgag gatctcgggc aggtccaggc ccctcccggc ctctacagcg
     4501 gcttctccgg cgtcgcctgg acggtggagc acctgcaggg gcggctgatc gagccggacg
     4561 acgatcctgg cgaggacatc gccgacgcga tggccgagca cctgtcgatc accccctggc
     4621 ggggggacta cgacctgatc agcggcctcg taggctacgg cgtctatgcc ctggagcgca
     4681 tgccccggcc ccagggggag gagtgcctgg cgcgggtggt ggcacggctg gacgagaccg
     4741 ccgagaaggg agcggactac gtgacctggc tcacgcggcc ggagctgctg ccgccgcgga
     4801 cccgggagga gaccccggag ggcaactaca acgcgggggt ggcccacggc gtgccggggg
     4861 tgatcgggct gctcggcgag gtctgcgccg ccggcgtggc gctcgagcag gcccggccac
     4921 tcctggaggg ctcggtctcc tggctcctgg accaccggct gccgcccggc gggttctcgg
     4981 tcttccccta ctccgtcatc cccggccggg agctgagctc gacgcgcctg gcctggtgct
     5041 atggcg
//
LOCUS       DASPJI010000596         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_551544, whole genome shotgun sequence.
ACCESSION   DASPJI010000596 DASPJI010000000
VERSION     DASPJI010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551544"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2451)
                     /locus_tag="VEL74_16635"
     CDS             complement(<1..>2451)
                     /locus_tag="VEL74_16635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64205.1"
                     /translation="LIGFFVNTLVLRTGLAGDPGFGELAARVREVTLDAYAHQDLPFE
                     MLVGELQPERDLSRTPLFQVMLTLQNAPMEPLELPGLRLEPLGAPGGTALFDLTLNIS
                     EDEAGIAGAAEYSTDLFEAVTIERLLTHLTVLLSAAVAVPDTPVASLPLLTEGERRQL
                     LLEWNDTGRPFPDHLGLHELVEQQAALAPGAFAVIDRGTDRGATLTFGDLDRRAGLLA
                     ARLRAHGVGPECVVAICVERSANLAVAILGVLKAGSAYLPLDPEHPLDRLAGLIEDSG
                     AALLLTREGLIERFSALGAAVLPVDGPAPSAPAPVRPVHPESSAYVIFTSGSTGRPKG
                     VVVPHQALVHYTLEMVRGMGLGPSDRVLQFAPVSFDVLAEELFPTWAAGGAVVFDDPD
                     RLRSPLQLHRAIEEAGVTWTELPAPYWQEWVRELESGRARFPSTLRLLVVGSEKPAPE
                     SLAVWQRFGVELIYIFGLTETTITSSFHRLAGGDDLDLPIGRPIANTSLYVLDGALEP
                     LPVAVPGELLIGGEGVARGYLGRPDLTAERFIPDPFAARPGARLYRTGDRARMRVDGS
                     VDFLGRLDHQVKIRGFRVEPGEIERRLEEHPAVRDAAVLVREDRLGDRRLVAYVVTEA
                     AEGDLREFLRQRLPEYMVPSAFVPLAALPLTPHGKLDRRALPAPEPEVARTSEGTAPR
                     SPVEEVLAGIFRDVLARDRVGIHDGFFDLGGHSLLATRVISRVRDAFGVELPLRRLFE
                     SPTVAGLAPEIAAILGSGPAAPPLVPVRRDQPLPLSFAQQRLWFLDRLEPGSSTYNVP
                     SAVRLDGAVDVAALAAALG"
BASE COUNT          383 a          869 c          906 g          293 t
ORIGIN      
        1 gcccagcgcc gccgccagcg ccgccacgtc cacggccccg tccaggcgga cggcggacgg
       61 cacgttatag gtggagctgc cgggctctag gcggtcgagg aaccagaggc gttgctgggc
      121 gaaggagagg ggcaacggct ggtcgcggcg gaccgggacc agaggcggag ccgccggtcc
      181 gctccccagg atcgcggcga tctccggcgc cagaccagcg acggtggggc tctcgaacag
      241 gcgacgcaga ggcagctcca ccccgaaggc gtcgcgcacc cgcgagatca cccgcgtcgc
      301 cagcagcgaa tggccgccga gatcgaagaa tccgtcgtga atgccgaccc ggtcccgcgc
      361 cagcacgtcg cggaagatgc ccgccaggac ctcctcgacg gggctgcgcg gcgcggtgcc
      421 ctccgaggtc cgggccacct cgggctccgg cgccggcagc gcccggcggt ccagcttccc
      481 gtggggcgtg aggggcaggg cggccagagg gacgaaggcg gacggcacca tgtactcggg
      541 gaggcgctgg cgcaggaact ctctgagatc cccctccgcc gcctccgtca ccacgtaggc
      601 gacgagccgc cggtccccca gccggtcctc gcggaccagc accgccgcgt cccgcaccgc
      661 cgggtgctcc tcgaggcgcc tttcgatctc gcccggctcg acccggaagc cccggatctt
      721 gacctgatgg tcgaggcggc cgaggaagtc gacgctgccg tccacgcgca tccgggcccg
      781 gtctcccgtg cgatagagcc gcgctccggg ccgggccgcg aaggggtcgg gtatgaagcg
      841 ctcggcggtg aggtccggcc ggcccaggta gccgcgcgcc acaccctccc cgccgatgag
      901 cagctccccc ggcacggcga cgggcagagg ctcaagtgcg ccgtccagca cgtagagcga
      961 cgtgttggcg atgggccggc cgatgggcag gtcgaggtcg tctccaccgg cgagacggtg
     1021 gaagctcgag gtgatggtgg tctcggtgag gccaaagata tagatcagct ccaccccgaa
     1081 gcgctgccag accgcgaggc tctcgggtgc cggcttctcg gagcccacga ccaggaggcg
     1141 gagcgtcgag gggaaccgcg cccggccgct ctccagctcc cgcacccact cctgccagta
     1201 gggggccggc agctccgtcc aggtgacgcc ggcctcctca atggcccggt gcaactggag
     1261 gggcgagcgg agccggtcgg ggtcgtcgaa caccacggcg ccgcccgcgg cccaggtcgg
     1321 gaacagctcc tccgccagca cgtcgaagct tacgggcgcg aactgcagta cccggtcgga
     1381 aggtcccagt cccatccccc ggaccatctc gagggtgtag tggaccagcg cctggtgcgg
     1441 caccaccacg cccttcggcc gcccggtgga gccggaggtg aagataacgt aggcggagga
     1501 ctcggggtgt acgggccgca ccggcgccgg ggctgaggga gcaggaccgt ccaccggcag
     1561 gacagcggca cccagcgctg agaagcgttc gatcagcccc tcgcgggtga ggagcagcgc
     1621 cgcgccggag tcttcgatga gcccggcgag gcggtccaag gggtgctcgg ggtccagagg
     1681 caggtaggcg ctgcccgcct tgaggacacc gaggatcgcc accgccaggt tcgcggagcg
     1741 ctcgacgcag atcgccacaa cgcactcggg ccccacgcca tgggcccgca gccgggcggc
     1801 gaggaggccc gcccgccggt cgaggtcgcc gaatgtcagg gtggcgccac ggtcggtgcc
     1861 gcggtcgatc accgcaaacg cgccgggcgc cagtgctgcc tgttgctcga cgagctcatg
     1921 gagaccgagg tggtctggga aggggcggcc ggtatcgttc cactccagca ggagctgccg
     1981 ccgctccccc tcggtcagga gcggcaggga ggcgaccgga gtgtccggaa ccgccacggc
     2041 ggcgctcagg agcaccgtga ggtgcgtcag caggcgctcg atggtgaccg cctcgaacag
     2101 gtcggtgctg tactcggcgg cccccgcgat ccccgcctcg tcctcgctga tgttgagcgt
     2161 gaggtcgaag agcgcggtcc cgcccggggc tcccaggggc tccagacgca gccctggcag
     2221 ctccagcggc tccatgggcg cgttctgcag cgtgagcatc acctggaaga gcggcgtccg
     2281 agagaggtcg cgctccggct gcagctcgcc caccagcatc tcgaacggta gatcctgatg
     2341 ggcataggcg tccagcgtca cctcccggac ccgggcggcg agctcaccga agccaggatc
     2401 ccccgcgagc ccggtgcgca ggaccagggt gttgacgaag aagccgatga g
//
LOCUS       DASPJI010000597         6537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_605896, whole genome shotgun sequence.
ACCESSION   DASPJI010000597 DASPJI010000000
VERSION     DASPJI010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605896"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VEL74_16640"
     CDS             complement(<1..332)
                     /locus_tag="VEL74_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HYG64206.1"
                     /translation="MEAHRVSEEELRLFLQRISRSLAERPVDLDPGTHREKVAALLRA
                     SGEATHYEFLGVELVARAMEIFEGFERTARLVHPGHAARLGLAGREGVLEVLFERATQ
                     AYLTLSNP"
     gene            382..1980
                     /locus_tag="VEL74_16645"
     CDS             382..1980
                     /locus_tag="VEL74_16645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYG64207.1"
                     /translation="MHRALRPLPWIAILLLAAAVRLTAVTAALPYMNYVDDGNYLHPV
                     AQMLRSGSWDPGWYLYPTLPLAAVAAAARLYGPVYQAEHGRPMREDLTPPGGDFYDVL
                     EPVELLLIGRVLCLIVSVGAVLLTGLLARRLAGNRAGLLAAFAAALVPALVIRSGIAT
                     VNPYAVFFTLACLLFADRLRTSSHPSREALAAGAMAGLAFASKYPAVLVSLSFAATVL
                     FVRSGWGERLRFWVLGGVGAVAGLVLGIPPLLTNPRGVLGGVFQQGTFYEELPSPSTF
                     WRQTFHRAEWDLPYEHPELGWPFLVLGLAGLALGAFTRDISGTVRAWWVYILVALGLY
                     TRYGYQPFRNLLPLVPLLCIGVALLAVRIRPRLPRPLWADAAGFLVIAALFGPPVTGY
                     ALERWRFQDSRKQAVDWLSAYTGPGDTVLVLRELAFLPGELGRLEAKIVPRRLEAART
                     PLLRAVPRFVLLGEPTNEQGRQAIGPAFRRRVLDRYEIRARFGEVNTPPFPYWWHGNR
                     QIVYVLERRDAAFPADLGAAPPWS"
     gene            complement(2371..2616)
                     /locus_tag="VEL74_16650"
     CDS             complement(2371..2616)
                     /locus_tag="VEL74_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HYG64208.1"
                     /translation="MDILTWIIVGLVAGVLASFVVGRGYGLLGDIVIGIIGAFVGGWL
                     FRQMGWGSPFGGLVGTIFVAFIGAVVLLLLLRLIRRA"
     gene            2792..5065
                     /locus_tag="VEL74_16655"
     CDS             2792..5065
                     /locus_tag="VEL74_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_function: GO:0003997 - acyl-CoA oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HYG64209.1"
                     /translation="MTPSTSSSPAEARRAALAEPRLAAYLPLVYIAWADGDLEPAEIA
                     DIRARVEAGDGLPAPARSLLCAWLDPWAAPSAGELATLRGSLRRAGRERQAAALQPVE
                     RETLADLEAALGVTGTEAARRLDGERQPPPPAPAAAPLDAAGLASFLDGAQRGTRERV
                     RRLLSTPELAYRSELGREAYRAQVLAWCRRLAAEGVAPLGLPREHGGGGDPAAFLAAF
                     ETLGHHDLSLLVKLGVQFGLFAGSIYQLGSERHHAAYLPAAARLDLAGSFAMTETGHG
                     SNVADLETAARYDPEAGTFEIHTPTPGARKDYIGGAARDARLAVVFAQLETAGERHGV
                     HAFLVPLRDERGGVLPGIQIEDCGEKPGLNGVDNGRIAFDRVRVPREALLDRFATVAA
                     DGAYTSPIASPGKRFFTMLGTLVGGRVSVALAALSAAKSALCIAVRYGDRRRQFGAAG
                     EPETLLLDYPAHQRRLLPRLAATYALHFALRDLAARYVEAVTGEADETARRSVEGLAA
                     GLKALSTWHATDTIQTCREACGGQGYLSVNRFGALRADSDVFTTFEGDNTVLLQLVAK
                     GLLSEYRKQFGDMGFVDLMRFVASRAGTAMRELNPIISRTTAPEHLRDPEFHLGALRW
                     REEHLLATVARRLKKRLDSGLEPTRAMVDCQTHLLATARAHVERIIVERAAEAIAAAP
                     AEHAAVLRPLLALDALARIEAGAGWLLEHGVLEAAKARAVRKQVDVLCCELRPLARGL
                     VDAFAIPDEVLAAPIAV"
     gene            complement(5062..5616)
                     /locus_tag="VEL74_16660"
     CDS             complement(5062..5616)
                     /locus_tag="VEL74_16660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HYG64210.1"
                     /translation="MSQYQIALLVGSLRRDSFNRQLAKALMALAPPELSFQQVRIDDL
                     PLYNQDDDSDPADTVKRLKGEITASQGVLFVTPEYNRSIPGVLKNAIDHASRPYGQSV
                     WAGKPAGVVGVSVGAPGTSMAQQHLRNILAYLDMPTLGQPEVFIQVKDGLFDEGGNLA
                     NAETKAFLQGWMDRYVAWVTKHAA"
     gene            complement(5644..>6537)
                     /locus_tag="VEL74_16665"
     CDS             complement(5644..>6537)
                     /locus_tag="VEL74_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HYG64211.1"
                     /translation="VAATYLLSREAAERIELAEATVQSREEGLRIARVRLDAGITSAL
                     DFRQAESLLTQAETELAGLRLARARSENTLAVLVGGPLAGPLPEPLPLARQAGSVTLA
                     AGLPSELLLARPDIRAAEERLRASRADIGAARAAFFPSTGLTGNLGFASAELGDLVGS
                     DGLTWSFGPFISLPIFNRGRLRGNLTVAEAREDAAIADYERTIQTAFREVADALAGRR
                     YLAEQVAAQERGTLAQRQIAELARIRYREGAVGYIEVLDAERNLFAAEQALLQLRRAE
                     VDNLVALYIALGGGALAAPQP"
BASE COUNT          887 a         2416 c         2285 g          949 t
ORIGIN      
        1 tcggggttgc tgagcgtcag gtaggcctgg gtggcgcgct cgaagagcac ctccagcacc
       61 ccctccctgc cggccaggcc caggcgcgcc gcgtggcccg ggtggacgag acgcgccgtc
      121 cgctcgaagc cctcgaagat ctccatcgcc ctggcgacca gctcgacgcc caggaactcg
      181 tagtgggtgg cctcgccgct ggcgcggagg agggccgcca ccttctcccg gtgggtaccg
      241 gggtcgaggt ccacgggccg ctccgcgagg ctgcgggaga ttctctggag gaacagccgg
      301 agctcctcct cggacacacg atgggcttcc atgagatggc gcctatgtta cagcaacggg
      361 accggtatag attgcgcacg catgcaccga gccctccggc ccctcccctg gatcgccatc
      421 ctcctcctgg cggccgccgt gcgcctcacc gcggtcaccg ccgccctccc ctacatgaac
      481 tacgtggacg acggcaacta tctccacccg gtggcgcaga tgctccgctc cggctcctgg
      541 gacccgggct ggtatcttta ccccacgctg cccctcgccg ccgtggccgc ggcggcgcgc
      601 ctgtacggcc cggtctacca ggccgagcac ggccggccga tgcgcgagga cctcaccccg
      661 cccggagggg atttctacga cgtgctggag ccggtggagc tgctgctcat cggacgagtc
      721 ctctgtctga tcgtcagcgt gggggccgtg ctgctgaccg ggctgctggc gcggaggctg
      781 gcgggcaacc gggccgggtt gctggccgcc ttcgccgccg ccctggtgcc ggcgctggtg
      841 atccgcagcg gcatcgccac ggtgaacccc tacgcggtct tcttcaccct cgcctgcctg
      901 ctcttcgccg accgcctccg cacctcgtcc catccgagcc gcgaggcgct ggcggcgggg
      961 gcgatggccg gcctggcctt cgcgtccaag tacccggcgg tgctcgtctc cctctccttc
     1021 gcggccaccg tcctcttcgt ccgcagcggc tggggggagc gactccggtt ctgggtgctg
     1081 ggcggcgtcg gagccgtggc cggcctggtc ctggggatcc cgccgctcct caccaacccc
     1141 cgcggcgtgc tgggaggagt cttccagcag gggaccttct acgaggagct gccttctccc
     1201 tcgaccttct ggcggcagac gttccaccgg gccgagtggg acctccccta cgagcacccg
     1261 gagctgggct ggccgttcct ggtcctcggg ctcgccgggc tggccctcgg cgcctttacg
     1321 cgggacatct ccggcaccgt ccgtgcctgg tgggtgtaca tcctcgtggc gctggggctc
     1381 tacacccgtt acggctacca gccgttccgc aacctgctcc ccctggtgcc gctgctttgc
     1441 atcggcgtgg cactgctggc ggtccgaatc cggccgcgcc tcccccggcc cctctgggcg
     1501 gacgccgccg gcttcctggt gatcgccgcg ctcttcggcc cgccggtgac cggctacgcc
     1561 ctggagaggt ggcgcttcca ggactcccgg aagcaggcgg tggactggct gtctgcctac
     1621 accggccccg gagacaccgt gctcgtcctg cgcgagctcg ccttcctccc cggagagctc
     1681 ggccggctgg aggcgaagat cgtgccgcgg cgccttgaag cggcgcggac gcccctcctt
     1741 cgcgccgtcc cccgcttcgt tctcctgggc gagcccacga acgagcaggg ccggcaagcc
     1801 atcggtcccg ccttccgccg aagggtcctc gaccgctacg agatccgcgc ccgcttcgga
     1861 gaggtcaaca cgcccccctt cccctattgg tggcacggca accggcagat cgtctatgtg
     1921 ctggagcggc gggatgcagc ctttcccgca gatctcggag ccgctccacc ctggagctga
     1981 gctgtcccac cctggtatca agctgtccaa cctcagggtg gaggtgctcc accatggaga
     2041 ggcgcctctc caccccgaag cggagccgtc ccaccctgga caccagaggt cctaccctgg
     2101 agcggagccg ctccaccccg agtcgggaag gctccggaat ctgtggcgac ggctccagcc
     2161 tgaaggcgaa cggcccctgg ctgaagcgga gccgctcctg gctgcatggg gatcgctcct
     2221 ggtcgaggtg ggacgatccc tgtccagggt gatgagcctc ctgcctgaag gcgagcggct
     2281 catggtcagg atggagaggc tccggactga aggcgagcgg ctccgccatc tgggggagaa
     2341 cccggcggcc gcggtccagg gtcgcccgtt tcaggcccgg cggatgagcc gcagcagcag
     2401 gaggaggacg acggcgccaa tgaaagcgac gaagatggtg ccgaccagcc ctccgaaggg
     2461 ggagccccac cccatctggc ggaacagcca gccgcccacg aaggcgccga tgatgccgat
     2521 gacgatgtcg cccagcaggc catagccgcg gccgacgacg aacgatgcga ggacgccggc
     2581 gacgaggccg acgatgatcc aggtcaggat gtccatccga gtgctcccct ctcgtgatcc
     2641 gggaccgggg ggttccggcc cggcgtccac tgctctacga cggactgccg gaccggatgt
     2701 gcacggcgga tgccaggacg ggggacgggt atgatgggga catgaggggg tgaccgcccg
     2761 gtcgagccgg gctcgcgaaa ggatcgacgc catgacgccg agcacctcgt cttctccggc
     2821 ggaggcccgc cgcgccgctc tcgccgagcc gcgcctggcc gcctacctac cactcgtcta
     2881 catcgcctgg gccgacggcg acctcgaacc ggccgagatc gcggacatcc gcgcccgggt
     2941 ggaggccggc gacgggctcc cggcgccggc ccggtccctc ctctgcgcct ggctcgatcc
     3001 atgggctgcc ccatcggccg gcgagttggc caccctgcgg gggagcctgc gacgcgccgg
     3061 ccgggagcgg caggcggcgg cgctccagcc ggtggagcgc gagaccctgg cggatctcga
     3121 agccgctctc ggcgtgaccg gcaccgaggc cgcgcggcga ctcgacggcg agcgccagcc
     3181 gccgccaccc gcgcccgcgg cggcgccgct cgacgccgct ggcctcgcca gcttcctcga
     3241 cggcgcccag cgcggcaccc gtgagcgggt ccgccgcctc ctctcgaccc ccgagctcgc
     3301 ctatcgctcc gagctcggcc gcgaagccta ccgcgcgcag gtgctcgcct ggtgccgccg
     3361 gctggcggcg gagggcgtcg ccccgctcgg gcttccccgc gagcatggcg gcggcggcga
     3421 cccggcggcg ttcctcgccg cgttcgagac gctcggccac cacgacctct cgctgctcgt
     3481 caagctcggc gtccagttcg ggctcttcgc cggcagcatc taccagctcg ggagcgagcg
     3541 ccatcacgcc gcctacctcc ccgccgcggc gcgcctcgac ctcgccggca gcttcgccat
     3601 gaccgagacc gggcacggct cgaacgtcgc cgacctcgaa acggccgccc gctacgaccc
     3661 cgaggccggc accttcgaga tccacacccc gaccccgggg gcgcgcaagg actacatcgg
     3721 tggcgcggcg cgggacgccc ggctcgccgt cgtcttcgcg caattggaga ccgccggcga
     3781 gcgccacggc gtgcatgcct tcctggtgcc gctacgcgat gagcgcggcg gcgtcctgcc
     3841 cggcattcag atcgaggact gtggcgagaa gcccggcttg aacggcgtcg acaacggccg
     3901 catcgccttc gaccgcgtgc gcgtgccgcg ggaggcgctc ctcgaccgct tcgccacggt
     3961 ggcggcggac ggcgcctaca ccagcccgat cgcgagcccc ggcaagcgct tcttcaccat
     4021 gctggggacg ctggtcggcg gacgggtgag cgtggccctg gccgcgctgt cggccgccaa
     4081 gtcggcgctg tgcatcgcgg tccgctacgg ggaccgccgg cgccagttcg gcgcggcggg
     4141 tgagcccgag acgctgctcc tcgactatcc ggcgcaccag aggcggctgc tcccccgcct
     4201 cgccgccacg tacgccctcc acttcgccct gcgcgacctg gcggcgcgtt acgtggaagc
     4261 ggtcacggga gaggcggacg agacggcacg ccggagcgtc gaggggctcg ccgccgggct
     4321 caaggccctg tccacctggc acgcgacgga caccatccag acctgccgcg aagcttgcgg
     4381 cgggcagggc tacctctcgg tcaaccgctt cggagcgctc agggcggaca gcgacgtctt
     4441 caccaccttc gaaggggaca acaccgtcct cctccaactg gtggcgaagg ggctcctctc
     4501 cgaataccgc aagcagttcg gcgacatggg gttcgtcgac ctgatgcgct tcgtcgcctc
     4561 ccgcgccggc acggcgatgc gcgagctgaa tccgatcatc tcgcggacca ccgcgccgga
     4621 gcacctgcgc gatcccgagt tccatctggg agcactgcgc tggcgcgagg agcacctgct
     4681 cgccaccgtc gcccggcggc tcaagaagcg cctcgactcc ggcctggagc ccacccgggc
     4741 gatggtcgac tgccagactc atctcctggc caccgcacgc gcccacgtgg agaggatcat
     4801 cgtcgaacgc gccgccgagg ccatcgcggc ggcgccggcc gagcacgccg cggtcctgcg
     4861 tccgctcctc gccctcgacg cgctcgcccg gatcgaagcc ggcgccgggt ggctgctcga
     4921 gcacggagtc ctcgaggcgg ccaaggcgcg cgcggtccgc aagcaggtcg acgtcctctg
     4981 ctgcgagctg cgccccctgg cgcgcggcct ggtcgatgcc tttgcgatcc ccgacgaggt
     5041 gctcgccgcg ccgatcgcgg tctaggcggc gtgcttcgtc acccaggcga cgtagcggtc
     5101 catccagccc tgcaggaagg ccttggtctc cgcgttggcg agattgcccc cctcgtcgaa
     5161 cagcccgtcc ttcacctgaa tgaagacctc gggctggccc agcgtcggca tgtccaggta
     5221 ggccaggatg ttgcgcaggt gctgctgcgc catggaggta ccgggggcgc cgaccgacac
     5281 gccaaccaca ccggcgggct tgccggccca tacgctctgg ccatagggac gggaggcgtg
     5341 atcgatcgcg ttcttcaaca cgccggggat cgaacggttg tactccggcg tgacgaagag
     5401 gacgccctgg gaagcggtga tctcgccctt caaccgcttg accgtatcgg ccgggtccga
     5461 gtcgtcatcc tggttataaa gcggcaggtc gtcgatccgc acctgctgga aagaaagctc
     5521 cggcggcgcc agggccatca gggctttggc cagttggcgg ttgaacgaat cacggcggag
     5581 gctgccgacc agcagggcga tttgatactg gctcatggga tcttccttcg atcatgggtt
     5641 acgttacggc tgaggcgccg cgagcgcgcc gccgccgagg gcgatataaa gcgcgacgag
     5701 gttgtccacc tcggcgcggc gaagctggag cagcgcctgt tcggcggcaa aaaggttgcg
     5761 ttcggcatcg agcacctcga tgtagccgac ggccccttcg cggtagcgga tccgcgccag
     5821 ctcggcgatc tggcgttgcg ccagcgtgcc gcgctcctgg gcggcgacct gctcggccag
     5881 gtagcggcgg ccggcgagcg cgtcggcgac ttcccgaaac gcggtctgga tcgtgcgctc
     5941 gtagtcggcg atggcggcgt cctcgcgcgc ctccgcgacg gtcaggttgc cgcgcaggcg
     6001 gccacggttg aagatcggaa ggctgatgaa ggggccgaag ctccaggtca gaccgtcgct
     6061 gccgacgagg tcgccgagct cggccgaggc gaagccgagg ttgcccgtca gcccggtcga
     6121 cgggaagaag gcggcgcgcg cggcgccgat gtcggcccgg gaggcgcgca gccgttcctc
     6181 ggcggcgcgg atgtcgggcc gggcgagcaa caactcggag gggagccccg cggccagggt
     6241 caccgagccc gcctgccggg cgagcggcag cggctcgggc aaaggcccgg cgagcggccc
     6301 gccgacgagg acggcgaggg tgttctcgct ccgcgcccgc gccaggcgca ggccggccag
     6361 ctcggtctcg gcctgcgtca gcagcgactc cgcctgccgg aagtcgaggg ccgaggtgat
     6421 gcccgcgtcg agccgcaccc tggcgatccg cagcccctcc tcgcggctct gcacggtggc
     6481 ctcggcgagc tcgatgcgct cggcggcctc gcgcgatagg agataggtcg cggccac
//
LOCUS       DASPJI010000598        11030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_95334, whole genome shotgun sequence.
ACCESSION   DASPJI010000598 DASPJI010000000
VERSION     DASPJI010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11030)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11030
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95334"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /gene="hisF"
                     /locus_tag="VEL74_16670"
     CDS             <1..722
                     /gene="hisF"
                     /locus_tag="VEL74_16670"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HYG64212.1"
                     /translation="GRVVKGVRFQDLVDQGDPAESALRYAMQGADEIVFLDIGAAPEE
                     RDTELDWVRRTAEQVFVPLTVGGGVRSEDDARRLLLSGADKVGINSAAVERPELIASL
                     SRRFGSQCVVLSVDAWRREGGWEVVTRGGRHPTGLDALDWSVQGVERGAGEILLTSID
                     RDGTQGGYDLDLLAAVTSRVRVPVIASGGAGTPEHLAQALAAGAAAVLAASIFHQGTW
                     TVAGVKRALAAAGFSVRLEAA"
     gene            719..1396
                     /gene="hisIE"
                     /locus_tag="VEL74_16675"
     CDS             719..1396
                     /gene="hisIE"
                     /locus_tag="VEL74_16675"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HYG64213.1"
                     /translation="MSLDPAALRYDDRGLVPVVVQDVVTGAVLMLAFADREAVEKTLA
                     TGQAHFWSRSRQSLWRKGETSGNVLEVVEVLPDCDGDALLVRARPAGPTCHRGTRSCF
                     EPNPAGLELGWLAAVLDERRHADPAESYTARLIARGIERVAQKVGEEGVETALAAVSA
                     AHHAGDPARRTALVGEAADLLYHLVLLLLGSGLRPEDVAAELSKRHRPSTRRELVESE
                     ARPSDAI"
     gene            1386..2831
                     /gene="trpE"
                     /locus_tag="VEL74_16680"
     CDS             1386..2831
                     /gene="trpE"
                     /locus_tag="VEL74_16680"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HYG64214.1"
                     /translation="MPSEIRRAVPHIREFLADSLTPLAVYRRLAETSPYRFLLESVAG
                     GERVSRFSFLGAGPREIYRLYPDRLELERGGRRKRLPGEPLEALRRVVNETVAEPTSI
                     PFTGGLVGYFGYDLIRMVERLPNRPPDPFDLPVALLARFDTLVIFDHAHQRVLAIANE
                     IEGEVGVAAAERQLARLSKLLTEEVAGGGVAMPGVSPGPVLTRARPNLDGAAFRRAVE
                     TAKEHIAAGDIFQVVLARRFKVPRRIDPLALYRALRMVNPSPYMVLFESPDVGLVGAS
                     SEMLVRKQGRHLETRPIAGTRPRGMDAAGDRSLADGLKGDSKERAEHVMLVDLGRNDL
                     GRVALPGSVRVSSFMDIERYSHVMHLVSSVEAELDQGRDGLDALLSCFPAGTVSGAPK
                     IRAMEIIDSLEPEARGPYAGAVGYLSYSGDVDTCITIRTLVVREDETSVTAGAGIVAD
                     SDPAAEERETENKAAGLLTAVALAEALEERA"
     gene            2828..3424
                     /locus_tag="VEL74_16685"
     CDS             2828..3424
                     /locus_tag="VEL74_16685"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HYG64215.1"
                     /translation="MILMVDNYDSFTWNLVQMLTAAGADVEVLRNDACDATAMLAREP
                     EGIVLSPGPGRPEDAGVCLDLIRRLTKQRSATPLLGVCLGHQALGVAFGATVDRAKRL
                     MHGKTSPVRHGGQGIFAGLPDPFEATRYHSLDVKEETLPGELEPLAWSDDDSLMGMRH
                     RELPLWGVQFHPESVLTASGPHLLSNFLGICRRHGDPA"
     gene            3421..4449
                     /gene="trpD"
                     /locus_tag="VEL74_16690"
     CDS             3421..4449
                     /gene="trpD"
                     /locus_tag="VEL74_16690"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HYG64216.1"
                     /translation="MTSREPIAPKEALRRLALGESLSRDETEDLFGQLMDGLVSDPMK
                     AALLVSLRMKGEAVSEITGAAAAMRRRVVPIPHTAEGVIDTCGTGGDGRGTFNISTAA
                     ALAAAAAGLSVAKHGNRSVSSRSGSADVLAALGVRIEVEPAVAGRAIDQVGIAFLFAP
                     LLHPAMREVMPVRRELGLRTIFNVLGPLTNPAGARRQLMGVYAESLVEPIGQVLRDLG
                     AEHAMVVHGSDGLDEITTTGPTRVSEVRGGEVRTYHLEPERFRIRRARLEDLAGGGPE
                     ENAALMHRVLAGEPGPLSDITALNAGAALYVGGRVPSIEQGVEQAREMLASGAAARKL
                     AALKGFFA"
     gene            4446..5255
                     /locus_tag="VEL74_16695"
     CDS             4446..5255
                     /locus_tag="VEL74_16695"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HYG64217.1"
                     /translation="MSGPPDILIRIAERRRQRIAEALGGGSSPELSSGPPLTPSDNAF
                     LGALAGRRRHGASAPPAIIAEVKMGSPRLGSLAGRVDPVAQARTYAENGATALSVVVE
                     PDFFHGGYDLLSACREASGLPAIAKDFVVDPLQLVWAHEAGADAVLLIASLYGADELA
                     WYAGLARSLDLVPLVETHDADDIARLSGEDWELVGVNNRDLRTFDVELERSIALLPLL
                     PPGALRVAESGIRDALDVLLLRDSGFDAFLIGESLLLADDPAAKLCELVGG"
     gene            5263..5928
                     /locus_tag="VEL74_16700"
     CDS             5263..5928
                     /locus_tag="VEL74_16700"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HYG64218.1"
                     /translation="METIGRVRVKVCGVIDPENARMAAESGADFLGLNFYPRSPRYVD
                     AGRAREIADAVRQLSGQHRDLSLVGVFVNSPAAEIEKISDRVGLDLVQLSGDETPAEV
                     APFAGRAIRVFRTGGDPGSDTLEAYGDVWGVLFDAPHSSLYGGTGESWGYESVAAVAD
                     RRRVFLAGGLGPDNAARAVAACRPYAIDVCSRVESAPGIKDPELLLRLFEEVRHGEDK
                     TPS"
     gene            5903..7189
                     /gene="trpB"
                     /locus_tag="VEL74_16705"
     CDS             5903..7189
                     /gene="trpB"
                     /locus_tag="VEL74_16705"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HYG64219.1"
                     /translation="MARTKPRPDPAGYFGEYGGRFVPETLMEPLGELARAYDRVSRQR
                     AFRRELADVLREFAGRPTPLYYAERLTKELGGARIYLKREDLLHTGAHKINNAVGQCL
                     LARRMEKERVIAETGAGQHGVATAAAAAYFGLACVVYMGSEDMRRQRLNVDRMHLLGA
                     EVVPVDSGSRTLKDAINESLRDWVTNVRTTHYVLGSVLGPDPYPRMVRDFHRVIGDEA
                     RVQLKAKAGTDAPDLAVACVGGGSNAIGLFTAFVDDRRVRLIGVEAGGRGSARGEHAV
                     RFGRSSLGVLHGTRTLVLQDLEGQIATTHSVSAGLDYPAVGPEHVWLHEQKRVEYTSV
                     TDEEAVAAFHLLARTEGILPALESSHALAEAVRHAPRLSNRRVVLVNLSGRGDKDVES
                     VVAFDREQSARPGEPESIASMVARRQPAAPRVGGRR"
     gene            7186..8007
                     /gene="trpA"
                     /locus_tag="VEL74_16710"
     CDS             7186..8007
                     /gene="trpA"
                     /locus_tag="VEL74_16710"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HYG64220.1"
                     /translation="MSAIDEVFARCEEEERAAFIPFLMAGDPSLESTAAQLEALAAGG
                     ADIIELGVPFSDPIADGPTIQRSATRALAAGAKMSAILDLVARHRDRLDVPVVLFTYF
                     NPIHVRGVARFAEQAAASGVDGVLCVDLPPEEGCRELVPALREQGVDAVYLLAPTSSR
                     DRMKRVADASSGFIYYVSRTGVTGEREALPAELLREVKRLRRRLPLPLAVGFGISNPA
                     QVAAVGEVADGVVVGSGLVRLVEEMGADPDLPAALEQRVRELSAPLRKALRRRPA"
     gene            8043..8711
                     /locus_tag="VEL74_16715"
     CDS             8043..8711
                     /locus_tag="VEL74_16715"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HYG64221.1"
                     /translation="MERKQERQIATLIHGRYLLDLPSQMEGSPLLVGFHGYGETADKH
                     MEELRRIPGASRWALCAVQALHPFYNRTGEVIASWMTRLDRDQAILDNIRYVSSVVAE
                     VRLQVPVSQRLAYLGFSQGAAMAYRAAASCGHSSQGVVVLGGDVPPELETRDLAHFPP
                     VLLGRGSSEEWYDAAKMDHDVGLLQRKGVHVQPFVFQGGHEWTNEFRAAAGEFLAQVF
                     SPAS"
     gene            complement(8795..9073)
                     /locus_tag="VEL74_16720"
     CDS             complement(8795..9073)
                     /locus_tag="VEL74_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64222.1"
                     /translation="MKKLFVHLSLLCLLAALLVPMVGCGRAEEGNTAVEETTGTTDMG
                     MGMEPAPMATDTTMGTDMGTGMGTDMGMGTDMGTDMGTGMGTATPPAQ"
     gene            complement(9125..9442)
                     /locus_tag="VEL74_16725"
     CDS             complement(9125..9442)
                     /locus_tag="VEL74_16725"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HYG64223.1"
                     /translation="MNKAMIRVSVVCFALMALLALGACSSTRTPGAQVDDAAITAAVK
                     AKLTADGDINPFNIDVDTNEGVVTLQGRVEKEEARTKAEQLARDTDGVRRVVNLITVG
                     DRS"
     gene            complement(9658..11001)
                     /locus_tag="VEL74_16730"
     CDS             complement(9658..11001)
                     /locus_tag="VEL74_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003274427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYG64224.1"
                     /translation="MPHALAVDDDPNFLSALAELIEGQGFTTNTALNLRDARAQVSHR
                     TPDVALVDLYLPDGSGIDLLKDLELGTGTEVVLMTGHADVESAVQALRLGASDYLTKP
                     LDIGRLKSILANVASLQQPSTTAMAPPPEAPEAGQQRLGLLLGASATMQSLYEMLTRV
                     APTDATVFLIGESGTGKDLAAQTLHQLSRRTKAPFLPLNCGAISPTLIESELFGHERG
                     SFTGAARRHKGYFERAHGGTLFLDEITEMPIELQVKLLRALETGTVVRIGGDQPTEVD
                     VRVIAATNRDPQKAVSDGKLREDLLYRLSVFPISMPPLRERGDDIPLLADYFLSQLNE
                     RQGTGKRFSEDAMERLRSHSWPGNVRELKNVVHRAFIMADQEINSRCLPREVGGDSGL
                     IRSLHFQVGTSIEEVERRLIMATLDAYGGNKRKTAEILGVSLKTLYNRLNGYRED"
BASE COUNT         1547 a         3850 c         3928 g         1705 t
ORIGIN      
        1 ccgggcgggt ggtcaagggg gtgcgcttcc aggatctggt cgaccagggg gacccggcgg
       61 agtcggcgct gcgctatgcc atgcaggggg cggacgagat cgtcttcctg gacatcggcg
      121 ccgcgcccga ggagcgcgac acggagctgg actgggtgcg ccgcaccgcc gagcaggtct
      181 tcgtccccct caccgtgggc ggcggcgtgc gcagcgagga cgacgcgcgg cgcctcctcc
      241 tctccggcgc cgacaaggtc gggatcaact ccgccgccgt cgagcggccg gagctcatcg
      301 ccagcctctc ccgtcgcttc ggcagccagt gcgtggtgct gtccgtcgat gcctggcgac
      361 gggaaggtgg ctgggaggtg gtcacacgtg gcggccgaca tcccaccggg ctcgacgcgc
      421 tcgactggag cgtccagggg gtggagcgcg gtgccgggga gatcctcctc accagcatcg
      481 accgcgacgg cacccagggg ggctacgacc tcgacctcct cgccgccgtg accagccggg
      541 tgcgggtgcc ggtcatcgcc tcgggtgggg cgggcacgcc ggagcacctg gcccaggctt
      601 tggccgctgg cgctgccgcg gtgctcgccg cgtccatctt ccatcagggg acctggaccg
      661 tggccggggt gaagcgagcc ctggccgccg ccggcttctc ggtccgtctg gaggccgcat
      721 gagtctcgat cccgccgccc tgcgctatga cgaccgaggt ctggtccctg tcgtcgtcca
      781 ggacgtggtc accggtgccg tcctgatgct cgccttcgcc gaccgcgagg cggtggagaa
      841 gaccctggcc accgggcagg ctcacttctg gagccgctcg cgccagtccc tctggcgcaa
      901 gggggagacc tccggcaacg tcctggaggt ggtcgaagtg ctgcccgact gcgacggcga
      961 cgccctgctg gtgcgcgccc ggccggcggg gcccacctgc caccgcggca cccggagctg
     1021 cttcgaaccc aatccggccg gcctggagct gggctggctc gctgccgttc tcgacgagcg
     1081 gcgccacgcc gatccggccg agagctacac cgcccgcctg atagcgcgcg gcatcgagcg
     1141 cgtggcacag aaggtaggag aggaaggggt ggagaccgct ctcgccgccg tctccgccgc
     1201 gcaccatgcc ggcgaccccg cccggcggac cgctctggta ggtgaggcgg ccgacctcct
     1261 ctaccacctc gtcctcctcc tcctggggag cggcctgcgg cctgaggacg tggcggcgga
     1321 gctttcgaag agacaccgcc cctccacccg gcgggagctg gtagagtccg aagcgaggcc
     1381 gtccgatgcc atctgagatc cgccgcgctg taccccacat ccgggagttc ctcgccgaca
     1441 gcctgacgcc gctcgcggtg taccggcgcc tcgccgagac ctccccctac cggttcctgc
     1501 tggagagcgt cgccggcggc gagcgggtct cccgcttcag cttcctcggc gccgggccac
     1561 gcgagatcta ccgtctctac cccgaccgtc tcgaactgga gcggggcggg cgccgcaagc
     1621 gactgcccgg cgagccgctg gaggcgctac gccgagtggt caacgaaacg gtggccgagc
     1681 cgacctctat cccgttcacc ggcggcctgg tgggctattt cggctacgac ctcatccgga
     1741 tggtggagcg cttgccgaac cgcccgccgg atccgttcga cctccccgtg gcgctgctgg
     1801 cccgcttcga caccctggtg atcttcgacc acgcccatca gcgggtgctc gccatcgcca
     1861 acgagatcga gggggaggtg ggggtggccg ccgccgagcg ccagctcgcc cgcctctcca
     1921 agctgttgac tgaagaagtg gcgggcggcg gcgtcgccat gccgggagta tctccgggcc
     1981 cggtcctgac ccgcgcccgc cccaacctcg acggagcggc gttccggcgg gcagtcgaga
     2041 ctgccaagga gcacatcgcg gccggcgaca tcttccaggt ggtcctcgcc cggcgcttca
     2101 aggtgccgcg ccggatcgac ccgctggcgc tctatcgcgc cctgcgcatg gtcaacccca
     2161 gcccctacat ggtgctgttc gagtccccgg acgtcgggct cgtcggtgcc tcgtcggaga
     2221 tgctggtccg caagcagggg cggcatctgg agactcggcc catcgccggt acccggccgc
     2281 gcggtatgga cgccgccggc gaccgcagcc tcgccgacgg cctcaaggga gattccaagg
     2341 agcgggccga gcacgtcatg ctggtggatc tggggaggaa cgacctcggc cgcgtcgccc
     2401 tacctgggag cgtccgggtg tcgagcttca tggacatcga gcgatacagc catgtcatgc
     2461 acctggtctc cagcgtcgag gccgagctcg atcagggaag ggacggcctc gacgcgctgc
     2521 tgtcctgctt cccggcgggc accgtgtccg gcgctcccaa gatccgggcg atggagatca
     2581 tcgattcgct ggagcccgag gctcggggac cctacgccgg cgccgtcggc tacctctcct
     2641 attccggcga cgtggacacc tgcatcacca tccgcacgct ggtggtccgc gaggacgaga
     2701 cctcggtcac tgccggcgcc ggcatcgtcg ccgactccga tccggcggcg gaggagcggg
     2761 agaccgagaa caaggcggcg gggctgctca cggctgtggc cctcgccgaa gcgttggagg
     2821 aacgcgcttg atcctgatgg tggacaacta cgactccttt acctggaacc tggtgcagat
     2881 gctgaccgcc gccggggccg acgtggaggt gctgcgcaac gacgcttgcg acgctacggc
     2941 catgctcgcc cgcgagcccg agggcatcgt gctctcgccc ggccccggcc gccccgagga
     3001 cgccggcgtc tgtctcgacc tcatccgccg gctcaccaag cagcggtcgg cgacgccgct
     3061 ccttggcgtc tgcctcgggc accaagccct gggcgtcgcc ttcggcgcca ccgtcgaccg
     3121 ggcgaagcgg ctgatgcacg gtaaaacctc gccggtgcgc cacggcggcc aggggatctt
     3181 cgccggcctg cccgacccgt tcgaggccac ccgctaccac agcctcgacg tgaaggagga
     3241 gacgctgccg ggggagctgg agcccctggc ttggagcgac gacgacagcc tcatgggcat
     3301 gcgtcaccgc gagctccccc tgtggggcgt gcagttccac ccggagtcgg tgctcaccgc
     3361 ttcgggaccg cacctgctgt cgaactttct cggcatctgc cggcggcacg gagatcccgc
     3421 atgacctccc gggagcccat cgctccgaaa gaggcgctgc gccgcctcgc cctcggcgag
     3481 agcctgagcc gcgacgagac cgaggacctc ttcggccagc tcatggacgg actggtgagc
     3541 gatccgatga aggcggccct gctggtgtcg ctgcggatga agggggaggc ggtctccgag
     3601 atcaccggcg ccgccgcggc catgcgccgg cgggtcgtac ccattcctca taccgccgaa
     3661 ggggtgatcg acacctgcgg caccggcggc gacggccgag ggacgttcaa catctcgacc
     3721 gccgcggccc tcgccgcggc ggcggccggg ctctccgtgg ccaagcacgg caaccgctcg
     3781 gtctccagcc gctccggcag cgccgacgtg ctggcggcgc tgggtgtgcg catcgaggtg
     3841 gagccggcgg tggcggggcg cgccatcgac caagtgggga tcgccttcct cttcgccccc
     3901 ctgctgcacc cggccatgcg cgaggtgatg ccggtacgcc gggagttggg gctacgtacc
     3961 atcttcaacg tcctgggccc gctgaccaat ccggccgggg cgcggcgcca gctcatgggg
     4021 gtctacgccg agagcctggt ggagcccatc ggtcaggtgc tgcgcgacct cggggcggag
     4081 cacgccatgg tggtgcacgg cagcgacggc ctcgacgaga tcaccaccac cggccccacc
     4141 cgcgttagcg aagtgcgggg cggcgaggtc cggacctacc acctggagcc ggagcgcttt
     4201 cgcatccgcc gcgcccgcct ggaggatctg gctggaggtg gcccggagga gaacgccgcc
     4261 ctgatgcacc gcgtgcttgc cggtgagccg gggccgctgt cggacatcac ggccctgaac
     4321 gccggcgccg cgctctacgt gggtgggcgg gtgccgtcca tcgagcaggg ggtcgagcag
     4381 gcgcgggaga tgctcgcctc cggcgccgcg gcccgcaagc tcgccgccct caaaggcttc
     4441 ttcgcgtgag cgggccgccg gacatcctga tccggatcgc cgagcggcgg cggcagcgca
     4501 tcgccgaggc gctcggcggt ggcagctcgc cggagctatc ctcaggaccg ccgctcaccc
     4561 cctccgacaa cgctttcctg ggcgcgctgg ccgggcgccg tcgccatggc gcttcggcac
     4621 cccccgcgat catcgccgag gtcaagatgg gatcgccgcg cctcggctcc ctcgccggcc
     4681 gggtcgaccc ggtggcgcag gcgcggacct atgccgagaa cggcgcgacc gcgctctcgg
     4741 tggtggtgga gcccgatttc ttccatggcg gctacgatct cctttcggcc tgccgcgagg
     4801 cttcgggcct gcccgccatc gccaaggact tcgtcgtcga tcccttgcag ctcgtctggg
     4861 cgcatgaggc cggagccgac gccgtcctac tcatcgcctc cctgtacggg gcggacgagc
     4921 tggcatggta cgccggcctc gcccgcagcc tcgacctcgt gccgctcgtc gagacccacg
     4981 atgccgacga catcgcacgg ctttccggcg aggactggga gctggtgggg gtcaacaacc
     5041 gcgacctgcg caccttcgac gtcgagctgg agcggtcgat cgccctgctc cccctcctac
     5101 cccccggtgc cctgcgggtg gcggagagcg gcattcgcga cgccctggac gtcctcctcc
     5161 tgcgcgacag cggcttcgac gccttcctga tcggcgagtc gctgttgctg gcggacgatc
     5221 ccgcggccaa gctgtgcgag ctggtggggg gctgaccgcc cgatggagac gatcggccgg
     5281 gtgcgggtga aggtctgcgg cgtcatcgat cccgagaacg cccggatggc cgcggaatcg
     5341 ggggcggatt tcctggggtt aaacttctat ccgcgcagcc cgcggtacgt cgacgccggc
     5401 cgggcgcggg agatcgccga cgcggtgcgt cagctatcag gacaacatcg ggatctctcg
     5461 ttggtaggtg tcttcgtcaa cagcccggcg gccgagatcg agaagatctc ggaccgggtc
     5521 ggcctggacc tggtgcagct cagcggcgac gagacgccgg ccgaggtggc gccgttcgcc
     5581 ggccgggcga tcagggtgtt ccgcaccggc ggtgaccccg gaagcgatac ccttgaggcc
     5641 tacggcgacg tctggggggt gctgttcgac gcccctcaca gttccctgta tggtggcacg
     5701 ggcgagagct ggggctatga gtccgtggct gcggtcgcgg accggcgccg ggttttcctg
     5761 gcgggcgggc tggggccgga caacgcggca cgggcggtgg ccgcctgccg gccgtacgcc
     5821 atcgacgtct gctcgcgggt ggagtcggct cccggcatca aggatcccga gctgctgctg
     5881 cggctcttcg aggaggtgag gcatggcgag gacaaaaccc cgtcctgatc cggcggggta
     5941 cttcggagag tacggcgggc gcttcgtccc cgagacgctg atggagccgc tgggcgagct
     6001 ggcccgggcc tacgaccggg tgagccggca gcgcgccttc cgtcgcgagc tggccgacgt
     6061 gctgcgcgaa ttcgcgggtc ggcccacccc cctctactac gcggagcgcc tcaccaagga
     6121 gctcggcggc gcgcggatct atctcaagcg cgaggacctg ctgcacacgg gcgcgcacaa
     6181 gatcaacaac gccgtcggcc agtgcctgct cgcccgcagg atggagaagg agcgggtgat
     6241 cgccgagacc ggcgccggcc agcatggcgt agccacggcg gcggcggccg cctacttcgg
     6301 tctggcctgc gtcgtctaca tgggcagcga ggacatgcgg cggcagcgcc tgaacgtcga
     6361 ccgcatgcac ctcctcggcg ccgaggtggt gccggtcgac tccggcagcc gcaccttgaa
     6421 ggacgcgatc aacgagtcgc tacgcgactg ggtcaccaac gtccgcacca cccactacgt
     6481 cctgggatcg gtgctcggcc cggaccccta cccccgcatg gtgcgcgact tccaccgcgt
     6541 catcggcgac gaggcccggg tgcagctcaa ggccaaggcc ggcaccgacg cgcccgactt
     6601 ggcggtggcg tgcgtcggcg gcggcagcaa cgccatcggc ctgttcaccg ccttcgtcga
     6661 cgaccggcgc gtccgcctca tcggcgtcga ggccggaggg cggggcagcg cccggggcga
     6721 gcacgcggtg cgcttcggcc gcagctctct gggggtcctc cacggcaccc gcaccctggt
     6781 gttgcaggac ctggaggggc agatcgccac cacccactcg gtctccgccg ggctcgacta
     6841 cccggccgtg gggccggagc acgtctggct ccacgagcag aagcgggtgg agtacacctc
     6901 ggtgaccgac gaggaggcgg tggccgcctt tcacctgctg gcccgcaccg aggggatcct
     6961 gccggccctc gagagctcgc acgccctcgc cgaggcggtc cggcacgcgc cgcggctgtc
     7021 gaaccggcgc gtcgtgctgg tgaacctctc cgggcgcggt gacaaggacg tcgaatcggt
     7081 agtcgccttc gaccgcgagc agtcggcgcg gccgggagag ccggagtcga tcgcatcgat
     7141 ggtggcgcgg cgccagcccg cggcaccacg ggtaggaggg aggcgatgag cgccatcgac
     7201 gaagtcttcg cgcgttgtga ggaggaggag cgggcggcgt tcatcccgtt cctcatggcc
     7261 ggggacccca gcctggagtc caccgccgcc cagctcgagg ctctggcggc ggggggcgct
     7321 gacatcatcg agctgggggt cccgttcagc gatccgatcg cggacggccc caccatccag
     7381 cggtccgcca cccgcgccct ggccgccggc gccaaaatgt cggcgatcct cgacctggtg
     7441 gccaggcacc gcgaccggct ggacgtacca gtcgtgctct tcacctactt caacccgatc
     7501 cacgtccggg gcgtggcacg cttcgccgag caggccgctg cctccggcgt cgatggcgtg
     7561 ctgtgcgtcg acctgccgcc ggaggagggg tgccgcgagc tcgtgccggc gctgcgcgag
     7621 cagggagtgg acgccgtcta cctgctcgcc ccgaccagct cgcgcgaccg tatgaagcgg
     7681 gtagccgacg cctccagcgg cttcatctac tatgtctcgc ggaccggggt gaccggcgag
     7741 cgcgaagcgc tgcccgccga gctgctgcgc gaggtcaagc gcctgcgacg gcggctgccg
     7801 ctgccgctgg cggtgggctt cggcatctcc aacccggcgc aggtggcggc ggtgggggag
     7861 gtcgcagacg gcgtggtggt ggggagcggc ctcgtccggc tggtggagga gatgggcgcg
     7921 gatccggacc tgccggcggc gctcgagcag cgggtgcggg agctgtccgc gccgttgcgg
     7981 aaggcgctgc gccggcgccc ggcgtgagga caccctggag acggagagaa ggaggtgagg
     8041 tggtggagag gaagcaagaa cggcagatcg cgacgttgat ccacggcaga tacctgctgg
     8101 acctcccgtc ccagatggag ggctcgcccc tgctggtggg cttccacggc tacggcgaga
     8161 cggccgacaa gcacatggaa gagctgcgcc gcatcccggg tgcctcgcgc tgggcgctat
     8221 gcgccgtcca ggcgctccac cccttctaca accggacggg agaagtcatc gccagttgga
     8281 tgacgcggct cgaccgggac caggcgatcc tcgacaacat ccgctacgtc tccagcgtcg
     8341 tggccgaggt ccggctgcag gttccggtct cgcagcgcct ggcctacctc gggttctccc
     8401 agggggctgc catggcctac cgcgccgccg cctcctgcgg ccactcgagc cagggggtgg
     8461 tggtgctggg cggcgacgta ccgcccgaac tcgagacgcg ggaccttgcc cacttcccgc
     8521 cggtgctgct ggggcgcggc agtagcgagg agtggtacga cgctgccaag atggaccacg
     8581 acgtcggcct gctccagcgc aagggggtcc acgtccagcc cttcgtcttc cagggggggc
     8641 acgagtggac caacgagttc cgcgccgcgg ccggggagtt cctggcccag gtcttctcgc
     8701 ccgcctcctg agcgaaaaac aaaaaggcgc ccggccagcg ggcgccctct cgtctgtgcg
     8761 gataagccgg tgtgactggc ttgtctgcgg tctcttactg agccgggggg gtggcggtgc
     8821 ccatgccggt acccatgtcg gtacccatgt cggtgcccat gcccatgtcg gtgcccatgc
     8881 cagtgcccat gtcagtgccc atggtcgtgt cagtggccat cggagcgggc tccatcccca
     8941 tgcccatgtc ggtggtgccg gtggtctcct cgaccgcggt gttcccctcc tcggcccggc
     9001 cgcaaccgac catcgggacg agaagagcag ccagcagaca caggagcgac agatgaacga
     9061 acagcttctt catgtgattt tctccctttt tgtatgaaac tgaattaaat gcggaggacg
     9121 accgctacga ccgatcgccc acagtgataa ggttgacgac tctccggacg ccatccgtat
     9181 ctcgggccaa ctgctccgcc ttggtccggg cctcttcctt ctcgaccctt ccctgcagcg
     9241 tcaccacgcc ctcattggtg tccacatcga tattgaacgg gttgatgtcc ccgtcggccg
     9301 tcagcttcgc cttcaccgca gccgtgatcg ccgcgtcgtc cacctgcgcg ccgggggtcc
     9361 tcgtcgagct gcaggcaccc agggccagca gcgccatcaa cgcaaagcat acgacagaaa
     9421 cgcggatcat tgccttgttc atgatgtcat cctcctcact ctatccttga ctacgttgtc
     9481 tgggggtctg tggcatcaga ccgtgtgtac gctcgttgct ctcaagcaga atttgtgcca
     9541 cagcctccag ccagggtgtc caggacgcgt acggagtgtc ggacggggcg agagtataca
     9601 gcctgccttg tcgctcctca agaggtgggg agcgcaaaaa ccgggattct tcggcatcta
     9661 gtcctcgcgg tagccgttga gccggttgta gagggtcttg aggctgactc ccaggatctc
     9721 cgcggtcttc cgcttgttgc cgccgtaggc gtccagcgtc gccatgatca ggcgccgctc
     9781 gacctcctcg atggaggtcc ccacctggaa gtggaggctg cggatcaggc cggagtcgcc
     9841 cccgacctcg cgcggcaggc agcgggagtt gatctcctgg tcggccatga tgaacgcgcg
     9901 gtggaccacg ttcttgagct cgcgcacgtt gccgggccag gagtggctcc gcaggcgctc
     9961 catggcgtcc tcggagaacc gcttgccggt cccctgccgc tcattgagct gggacaggaa
    10021 gtagtcggcg agcagcggga tgtcgtcgcc gcgctcacgc agggggggca tggagatggg
    10081 gaagaccgac agccggtaga ggaggtcctc gcgcagcttg ccgtcgctca ccgccttctg
    10141 cgggtcgcgg ttggtggcgg cgatgacccg cacgtccacc tcggtcggct ggtcgccgcc
    10201 gatgcgcacc acggtgccgg tctcgagcgc ccgcagcagc ttgacctgga gctcgatcgg
    10261 catctcggtg atctcgtcca ggaagagggt gccgccgtgg gcgcgctcga agtagccctt
    10321 gtgccgccgg gccgcgccgg tgaagctgcc gcgctcgtgg ccgaacagct cgctctcgat
    10381 cagggtcggc gagatggcgc cgcagttcag cggcaggaag ggcgccttgg tccggcgcga
    10441 cagctggtgc agcgtctgcg ccgccaggtc cttgccggtg ccgctctcgc cgatcaggaa
    10501 gaccgtggcg tccgtcgggg cgacccgcgt cagcatctcg tagagcgact gcatggtggc
    10561 cgaggcgccc aggagcaggc ccagccgctg ctggccggcc tccggcgcct ccggcggtgg
    10621 ggccatggcg gtggtggacg gctgctgcag gctcgccacg ttggccagga tgctcttgag
    10681 gcggccgatg tccagcggct tggtcagata gtcgctggcc cccaggcgca gggcctggac
    10741 ggcgctctcg acgtcggcgt gcccggtcat cagcaccacc tcggtcccgg tccccagctc
    10801 cagatccttg agcaggtcga tgccgctgcc gtcggggaga tagaggtcga ccagcgccac
    10861 gtccggggtc cggtggctca cctgggcccg ggcgtcacgc aggttcaggg cggtgttggt
    10921 agtgaagccc tggccctcga tcagctcggc gagcgcggaa aggaagttgg gatcgtcgtc
    10981 gaccgcgagt gcgtggggca tctcctactc cttgtcggag gcaacagcag
//
LOCUS       DASPJI010000599         7878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_785336, whole genome shotgun sequence.
ACCESSION   DASPJI010000599 DASPJI010000000
VERSION     DASPJI010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785336"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VEL74_16735"
     CDS             complement(<1..447)
                     /locus_tag="VEL74_16735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HYG64225.1"
                     /translation="MDQMRLSVVIPCLNAAATLPDQLEALVNEATDQSWEGGWEVIVA
                     DNGSTDGSREVVERFRARLPELRLVDASDRRGQAHARNVGAAAATGDAFLFVDADDAV
                     APGWLGAMGRALSRHGFVACRYDNEKLNPVWVQRTHLNPQKDGLTSY"
     gene            620..2236
                     /locus_tag="VEL74_16740"
     CDS             620..2236
                     /locus_tag="VEL74_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64226.1"
                     /translation="MFPTQDGPSHQAVAQVLRLYDLPEGEFLHEYYLRNREASPNEFI
                     FFLMARVLGFVPVPAAEKILLSVYVLLLPLSVLYALRAVAPRDAPGTVALAALAFPFT
                     YNFLMQMGFFNFCFSLAAFFFALGFWWRRQGRLGLLNGTVFAVLVLWVYFCHVVSMVM
                     LLAVVGTVAAWRVLVDFRGRSSEVSAAGALREGFRRWLVVPLLAFGPALALMAWFVGQ
                     RVDARVTTMPLSVKVKHLGGLYSLVSLDANTVALSAVLAALFAGVTAVLVARRLRRGG
                     LLAADGLFLAVLVATVAYFAAPSEMSGGGFLVHRLNLYPFLLLIPWFATFDLGRRAAL
                     GIQAMAALIAVALLSLFWPRWEKLNGELSEYLTAADRIEAGSTLLPLVFDHRGKTPEG
                     TIAFRTRPFLHASGYIAARKPVVDLALYEAKESYFPLLFRPDRDPYTRIGIGPGLGLE
                     GEPPRVDFLRYPELTGGRVDYVLLSGLDAAPPDHPDVRSIQNQLAAAYEPVYVSPRRL
                     VRLYRHREAAGFMAARAKTATGAEILAASR"
     gene            complement(2178..3284)
                     /locus_tag="VEL74_16745"
     CDS             complement(2178..3284)
                     /locus_tag="VEL74_16745"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HYG64227.1"
                     /translation="MAGERRKKLILATLSVVLTLLLAEAALRILGIGAAGRGSAWFAG
                     GNHPCFLFKPDPASGYTLRPGFRGVEISPAGEFQVTAAIDGRGLRDHPHSAPPRPVVL
                     ALGDSMTFGEGVPVGDAYPALLEKALGVRVYNGGVPGYGSPQMLGRLERLQPVLRPDA
                     VLVTLSPYWDARRGVTPFVYKDGYIVAQSYLDRLHLINGNLYHAETRLPVLGPLTAQA
                     KGHSNLMRLALPPLFERVHRLQEKEGASGGVVERVDPEPTLRTVVEAKRRAEQAGAGF
                     LLILLDSDTRGDEYRQAHLDLTQELRRRGFPYVDLQELLPGTDWPRLRYPWDAHWTAE
                     GHRVVAAALASPVRDLIGRRPGSRPPSPSSPSRP"
     gene            complement(3288..5135)
                     /locus_tag="VEL74_16750"
     CDS             complement(3288..5135)
                     /locus_tag="VEL74_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64228.1"
                     /translation="MKSTLSWAVLSLLLLAALVGAATFDRRAWPAMVGDEATYLMQAQ
                     SLAWDFDLRYTRKDFDRFKAQWGVPPEGLILQRDGDGLVYGKPSSYPLFIAPFLSLSP
                     TRGASVANVLILALAAILTARTLERRIGPAAPLWVATWIFTSVAFVYIFWIHSDLFLM
                     SLVAIALSLAYGEGGRVPARPAQATGSEGGGEGGGAHTKEPSPAEPVQDETGNTDPGP
                     AEGGRRPLLRWLLVGVLLGIVIMARPFYATLLLPTALAIPPVYRRRGLAALIAGAAGL
                     LLLAAATNLLSRGHWTSYTGDREAFYSYTGFPQVDLSERDWEERVAEQSSNSWIAGAT
                     NQVDVVSRQTGWNVVYFLFGRHVGLLPYFLPALLGFMAWRRGEGRWALILAAAATAAC
                     FLIIRPFNFWGGGGAIANRYFLPVYPALWFVAARPARAVWALVIGALAAPLLWPLWTA
                     PRSFPIDADGGYRHVSPVARRVLPYETTLSHLKPAGQEDQIHNGLWVKLLSPSLRVEE
                     GGSQLRLLAGRGGEVLVGSSTPLPGLRLRFAPSPVTRMRVVGPEVAQVVRRPDGGTTL
                     LLRFRHPRAHHRMWWGEDDLYLYQFGLEPPEGAETPPDGLAFQILPETS"
     gene            5190..6179
                     /locus_tag="VEL74_16755"
     CDS             5190..6179
                     /locus_tag="VEL74_16755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYG64229.1"
                     /translation="MSTQPLARIGPPTPAAIPAEVDVSVLIPVRDEAGTVLELSRQVA
                     DVLERLGRSFEIIFVDDGSSDGTHLRVREARERDPRVKLVRLRRNFGKAAALCAGFDY
                     SNGAIVITMDGDLQDDPEEIPRFLAVLEEQELDLVSGWKRRRQDPASKRLPSLLFNWV
                     TRRLAQVELHDFNCGFKAYRREVLAEIAVYGELHRYIPVLASRRGFAVGEIQVKHHAR
                     RYGVSKYGWDRLYKGLLDLITVLFITRYTRRPLHLFGAVGLVFLAFGLGINSYLAVAW
                     FGGADLSDRPLLQLGILLMLLGIQVLTTGLIGEMITFKNFRRRDSYSIKEWLE"
     gene            6176..7435
                     /locus_tag="VEL74_16760"
     CDS             6176..7435
                     /locus_tag="VEL74_16760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG64230.1"
                     /translation="MSPSVPDRLDLLFLTQTYPRFEGDTAGPFIRDLARGLARIGDRV
                     TVLAPHTAGLPPVWQEQGVEVVTFRYAPERFELLGYGRSLQADERVKGGAALVAPLYA
                     LGARRELARLLRLLRFDLLHAHWIVPNGLVAATLKATSSKVPLAVGLHGSDVFLAEKP
                     GVRQMVRRVLARTRLLTGPSPELVDRVRALGLPEERTRVIPYGVDVRTFAPAPARRRI
                     WRELLGIPAEAPLLLGVGRMATKKGFQILVEILPELLAQSPELHVILAGGGDRLAEFA
                     AAAERWPGRVHFPGPVLRDTLPDLYRAADVFTLPAVHDPEGNVDGLPNVILEAMASGL
                     PVVTTGISGIPLAVSHGETGLLVPEGDAEALLEALLRLLEDRALARRMGEKGRRRAEA
                     ALTWDAVAARYREGYVAALSGPLSVRV"
     gene            7502..7837
                     /locus_tag="VEL74_16765"
     CDS             7502..7837
                     /locus_tag="VEL74_16765"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HYG64231.1"
                     /translation="MTTKKDSFDRSGLTKADLVNLVYDRHGGLTKDEAAEIVDAIFST
                     VKRSLGRGRTVRIQNFGTFEITSRKGRTGVNPVSGEKIFIRPQKGLSFRPARRLKDEL
                     VQRGPRKGE"
BASE COUNT         1124 a         2724 c         2779 g         1251 t
ORIGIN      
        1 gtaggaggtc aggccgtcct tctggggatt gagatgggtc ctctggaccc agaccggatt
       61 gagcttctcg ttgtcatacc ggcaggcgac gaagccgtgc cgcgagagtg ctcgccccat
      121 ggccccgagc cagccggggg ccaccgcgtc gtcggcatcg acgaacagaa aggcgtcgcc
      181 cgtcgccgcg gcggcgccca cgttccgggc gtgcgcctgc ccccggcggt ccgaggcgtc
      241 taccagccgc agctccggga gccgggcgcg gaaccgctcc accacctccc gcgagccgtc
      301 cgtcgagcca ttgtccgcca cgatcacctc ccacccacct tcccagctct ggtccgtggc
      361 ttcgttgaca agggcctcga gctgatcggg gagggtcgcc gccgcgttaa ggcaggggat
      421 gaccacgctg agcctcattt ggtccattcc gtgcatgcat cttagtcgat gcttctggtg
      481 atctgaaagt ccctgtctgg aggatcttgc cgtggacggc cctgtagtac ccggtcagcg
      541 gctcggttgg cgcagccccg agtccctgat tttcatgctc ctgctggttc ttcatctggt
      601 tcccatctgg agcctgcgga tgtttccgac gcaggatggc cccagccacc aggccgtggc
      661 ccaggtgctc cgtttgtacg atcttccaga aggcgagttc ctgcacgagt actatttacg
      721 taaccgcgag gcctcgccca acgagttcat cttcttcctg atggcccggg tcctcggatt
      781 cgtcccggtg ccggcggccg agaagatcct gctcagcgtc tacgttctcc tgctgccgct
      841 gtcggtcctc tacgccttac gggcggtggc gccgcgcgat gctcccggca cggtggcgct
      901 cgccgcgctg gccttcccgt tcacctacaa cttcctgatg cagatggggt tcttcaactt
      961 ctgcttcagc ttggccgcgt tcttcttcgc tctgggattc tggtggcggc gccaggggag
     1021 gctggggctc ctcaacggga ccgtcttcgc ggtcctggtg ctgtgggtct acttctgtca
     1081 cgtagtctcc atggtgatgc tgctggcggt ggtcggcacc gtggcggcct ggcgggtgtt
     1141 ggtcgacttc cgaggccggt cctccgaggt ctcggccgcg ggcgcgttgc gggaaggttt
     1201 ccggcgatgg ctggtggtgc cgcttctggc cttcgggccg gcgctggcgc tcatggcctg
     1261 gttcgtcggc cagcgcgtgg acgcgcgggt caccacgatg ccgctgtcgg tcaaagtcaa
     1321 gcacctgggc ggcctctact ccctggtgtc tctcgacgcc aacacggtgg cgctctcggc
     1381 cgtgctggcc gcgctctttg ccggggtgac ggcggttctc gtggctcggc ggctccggcg
     1441 tggcgggctc ctcgccgccg acggcctgtt cctcgccgtc ctcgtggcca ctgtggccta
     1501 cttcgcggcg cccagcgaga tgagcggcgg cggcttcctg gtccaccggc tcaacctcta
     1561 tccctttctc ctgttgatcc cctggttcgc gaccttcgac ctcggacgcc gcgcggcgtt
     1621 gggcatccag gcgatggcgg ccctgatcgc cgtggcgctc ctctccctct tctggccgcg
     1681 ttgggagaag ctgaacggcg agctgtccga gtatctgacc gcggccgacc ggatcgaggc
     1741 ggggagcacc ctgctcccac tggtctttga ccatcggggc aagacgcccg aagggacgat
     1801 cgccttccgg acccgcccct tcctgcacgc ctccggctac atcgccgccc ggaagccggt
     1861 ggtcgacctg gcgctctacg aagccaagga gagctatttc cccctgctgt tccggccgga
     1921 tcgcgacccc tacacccgca tcgggatcgg tccgggtctc gggctggaag gcgagccgcc
     1981 gcgggtggac ttcctgcgct acccggagct gaccggagga cgggtggact atgttctcct
     2041 ctccggactc gacgccgcgc cgccggacca tccagacgtg cggtccatcc agaaccagct
     2101 cgccgccgcc tacgagccgg tctacgtctc gccgcgccgc ctcgtccggc tctatcgtca
     2161 ccgggaggcg gccggcttca tggccgcgag ggcgaagacg gcgacggggg ccgagatcct
     2221 ggccgcctcc cgatgaggtc tcgcacagga gaggcgaggg ccgcggccac cacccggtga
     2281 ccctccgcgg tccagtgggc atcccaggga taacgcagcc ggggccagtc ggttccggga
     2341 agaagctcct gcagatcgac gtaggggaag ccgcgccgcc gtagctcctg cgtcagatcg
     2401 aggtgagcct gccggtactc gtcgccccgg gtgtcgctgt cgaggaggat cagcaggaac
     2461 cccgcccctg cctgctcggc tcgccgcttc gcctcgacga cagtgcggag ggtgggctcg
     2521 ggatcgaccc gctctacgac cccgccgctc gccccttcct tttcctgaag gcgatgcacc
     2581 ctctcgaaca ggggaggcag ggcgagccgc atcaggttgg agtgcccctt cgcctgggcg
     2641 gtgagagggc cgaggaccgg cagccgggtt tcggcgtgat agagattgcc gttgatcagg
     2701 tggagccggt cgagatagct ctgggccacg atgtagccgt ccttgtagac gaacggcgtg
     2761 acgccgcggc gggcgtccca gtagggagag agggtgacga ggacggcgtc ggggcggagc
     2821 accggctgga gccgctcgag tctccccagc atctgtgggc tgccgtaacc gggtacgccg
     2881 ccgttgtaga cgcggacccc gagggccttc tccaggagag ccgggtaggc gtcgcccacc
     2941 ggtacgccct cgccgaaagt catggaatct cccagggcga gcacgacggg gcggggcggg
     3001 gccgaatggg ggtggtcgcg gagcccccgg ccatcgatgg cggcggtcac ctggaactcc
     3061 ccggccggcg agatctccac cccccggaag ccggggcgga gggtatagcc gctcgccgga
     3121 tcgggtttga agaggaagca ggggtgattg cctccggcga accacgccga gccccggccg
     3181 gcggcgccga tgccgaggat gcgcagggcc gcctcggcga gcagcaacgt gagcacgacc
     3241 gagagggtgg cgaggatgag cttcttcctg cgctctccgg ccatgggtca ggaggtctcc
     3301 ggcaggatct ggaaagcgag cccgtcgggg ggagtctcgg ccccttccgg cggctccagg
     3361 ccgaactgat agagatagag gtcgtcctcg ccccaccaca tgcggtgatg ggcccgggga
     3421 tgacggaacc gcaggaggag cgtcgtgccg ccgtcggggc ggcggaccac ctgcgccacc
     3481 tccgggccga ccaccctcat gcgggtgacg ggtgacggcg cgaaccggag tcgcaggccc
     3541 ggcagcggcg tgctgctgcc caccaggacc tcgccgcccc ggccggcgag gaggcggagc
     3601 tgggagcccc cctcctccac ccgcaggctc ggcgagagga gcttcaccca caggccgttg
     3661 tggatctggt cctcctggcc ggcgggcttg aggtgcgaca gggtggtctc ataggggagg
     3721 acgcgacgcg ccaccggcga gacgtgccgg tacccaccgt cggcgtcgat ggggaacgag
     3781 cgcggagcgg tccagagcgg ccagagcagg ggagcggcca gagcaccgat gaccagggcc
     3841 cagacggcac gcgcgggccg ggcggcgacg aaccagaggg cggggtacac cggcaggaag
     3901 tagcggttgg cgatcgcccc gccgccgccc cagaagttga agggacggat gatcaggaag
     3961 caggccgccg tggccgccgc ggcgaggatc agcgcccatc ggccctctcc ccggcgccag
     4021 gccatgaagc ccaggagcgc cggcaggaag taggggagga gccccacgtg ccggccgaac
     4081 aggaagtaga cgacgttcca gccggtctgg cggctcacca cgtccacctg gttggtcgcg
     4141 cccgcgatcc aggaattgct gctctgctcc gcgacccgct cctcccagtc gcgttccgag
     4201 aggtcgacct gtggaaagcc ggtataggag tagaaggcct cccggtcgcc ggtgtagctc
     4261 gtccaatggc cgcgggagag caggttggtg gcggcggcga gcaggaggag accagcggcg
     4321 ccggcgatga gagcggcgag gcctctccga cggtagacgg gagggatggc cagggccgtg
     4381 gggaggagca gggtggcata gaaggggcgc gccatgatga cgatgcccag gaggacaccg
     4441 acgaggagcc agcggagtag ggggcgccgg ccgccctcgg cgggtcccgg gtcggtgttt
     4501 cctgtctcgt cctggaccgg ctcggctggg ctcggctcct tcgtgtgcgc tcctccccct
     4561 tcccccccgc cctcgctgcc agtcgcctgc gccggtcgag ccgggacccg ccccccctcc
     4621 ccgtaggcta aggagagggc gatcgcgacc agggacatca ggaagaggtc ggagtggatc
     4681 cagaagatgt agacgaaggc gacggaggtg aagatccagg tggcgaccca gaggggggcc
     4741 gcggggccga tccggcgctc gagggtgcgc gcggtgagga tcgcggcgag cgccaggatc
     4801 aggacgttgg ccaccgaggc gccgcgggtg ggggagaggc tcaggaaggg ggcgatgaag
     4861 aggggatagg acgagggctt gccgtagacc aggccgtcgc cgtcccgctg caggatcagc
     4921 ccctcgggtg ggacgcccca ctgggccttg aagcggtcga agtccttccg ggtgtagcgg
     4981 aggtcgaaat cccaggcaag gctctgggcc tgcatgagat aggtggcctc gtcgcccacc
     5041 atcgccggcc aggcgcggcg gtcgaaggtg gcggctccga ccagggccgc caggagaagg
     5101 aggctcagca cggcccagct cagggtggac ttcatcgcgc gcattctatc cgggacaccg
     5161 cccccgaagg acgttacaat ggcacctcga tgagcaccca gcccctcgcc cgcatcggtc
     5221 cgcccacccc tgccgccatc cctgcggagg tggacgtgag cgttctgatc ccggtgcggg
     5281 acgaggcggg gacggtcctc gagctgtcca ggcaggtagc ggacgtcctc gagcgcctgg
     5341 ggcgcagctt cgagatcatc ttcgtggacg acggctcgag cgacggcacc catctgcggg
     5401 tgcgcgaggc acgggagcgc gatccccgag tcaagctcgt gcgcctgcgc cgcaacttcg
     5461 gcaaggccgc ggccctctgc gccggcttcg actactcgaa cggcgctatc gtcatcacca
     5521 tggacggcga cctccaggac gacccggagg agatcccccg cttcctcgcg gtcctcgagg
     5581 agcaggagct ggacctggtc tccggctgga agcgccgccg ccaggacccg gcaagcaagc
     5641 ggctgccgtc gctcctcttc aactgggtga cccgccggct ggcccaggtg gagcttcacg
     5701 acttcaactg cggcttcaag gcctaccgcc gggaggtgct ggcggagatc gccgtgtacg
     5761 gcgagctcca ccgctacatc ccggtgctcg ccagccgccg cggcttcgcg gtcggggaga
     5821 tccaggtcaa gcaccacgcg cggcgctacg gcgtcagcaa gtacggctgg gaccggctct
     5881 acaaagggct cctggatctc atcaccgtcc tcttcatcac ccgctacacg cggcggcccc
     5941 tccacctgtt cggtgccgtc ggcctggtct tcctggcctt cggcctcggg atcaactcct
     6001 acttggcggt ggcctggttc ggcggcgcgg acctctccga ccggccgctc ctccagctcg
     6061 gtatcctgct gatgctcctc ggcatccagg tgctgaccac cggcctgatc ggcgagatga
     6121 tcacgttcaa gaacttccgg cgccgcgaca gctactcgat caaagagtgg ctggagtgag
     6181 cccctcggtc cccgaccggc tcgacctcct cttcctgacg cagacctacc cgcgcttcga
     6241 gggggacacg gccgggccgt tcatccgcga cctcgcccgc ggtctcgccc ggatcggcga
     6301 ccgggtgacg gtgctggcgc cccacaccgc cgggctgccg ccggtctggc aggagcaggg
     6361 ggtggaggtg gtcacctttc gctacgcccc cgagcgcttc gagctcctcg gctacggccg
     6421 gagcctccag gccgacgagc gggtcaaggg gggcgccgcg ctggtcgccc ccctctacgc
     6481 cctgggcgcc cgccgcgagc tcgcccgcct gctgcgcctc ctccgcttcg acctgctgca
     6541 cgcccactgg atcgtgccca acggcctcgt cgcggcgacg ctcaaggcca cctcctccaa
     6601 ggtgcccctg gccgtcgggc tgcacggcag cgatgtcttt ctggccgaga agccgggcgt
     6661 ccgccagatg gtgcgccggg tcctcgcccg cacacgcctg ctcaccggcc cgtcgcccga
     6721 gctggtggac cgcgtccgcg ctctgggcct ccccgaggag cgtacccggg tcatccccta
     6781 cggcgtcgac gtccgcacct tcgcgccggc gccggcgcgg cggcggatct ggcgcgagct
     6841 gctgggtatc ccggccgagg cgccgctcct cctgggtgtc gggcggatgg ccaccaagaa
     6901 gggcttccag atcctcgtcg agatcctgcc ggagctcctc gcccagagcc cggagctgca
     6961 cgtcatcctc gccggtggcg gcgaccggct ggcggagttc gccgccgcgg ccgagcgctg
     7021 gccgggccgg gtccacttcc ccggacccgt gctgcgcgac accctgccgg acctctaccg
     7081 ggcggccgac gtcttcaccc tgccggcggt gcacgatccg gagggcaacg tcgacggcct
     7141 gcccaacgtc atcctggagg ccatggcgag cggcctgccg gtggtcacga ccggcatctc
     7201 cggcatcccg ctcgccgtct cccacggcga gaccggactg ctggtgcccg agggggacgc
     7261 ggaggccctg ctcgaggcat tgctgcgcct gctggaggac cgggcgctcg cccgccgcat
     7321 gggggagaag gggcggcgtc gcgccgaagc cgccctcacc tgggacgcgg tcgcggcgcg
     7381 ctatcgggag gggtacgtgg ccgccctgtc cggtcccttg tcggtgcggg tttgatcccc
     7441 tatacttcgt gtactccttt cgccattgtg gccgttcggc gaaatcccga tgtacggtct
     7501 catgacgacg aaaaaggact cgttcgatcg ttccggcctc accaaggccg atctcgtcaa
     7561 tctggtctac gaccgccacg gcggcttgac caaggacgag gcggcggaga tcgtggacgc
     7621 catcttctcg acggtgaaga ggtccctggg ccgcggccgt accgtccgca tccagaactt
     7681 cggcaccttc gagatcacca gccggaaggg gcgcacgggc gtcaacccgg tgagcggcga
     7741 gaagattttc atccgcccgc agaagggcct gagcttccgt ccggcgcgcc gtctcaagga
     7801 cgagttggtc cagcggggcc cacggaaggg cgagtagtct gaatggggtc gaagctgagg
     7861 gtccagaagc gtctctac
//
LOCUS       DASPJI010000600         4438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_208724, whole genome shotgun sequence.
ACCESSION   DASPJI010000600 DASPJI010000000
VERSION     DASPJI010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208724"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VEL74_16770"
     CDS             complement(<1..822)
                     /locus_tag="VEL74_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYG64232.1"
                     /translation="MSPVHGIHHVTAIAGDPRENLHFYTEVLGLRLVKRSVNQDAPDT
                     YHLFYADAEGHPGTDLTFFPWPSMGAGRTGTGLAVEVGFAVPEGSLAFWEERLESMRV
                     GPAIAASRFGERVLAFPDVHGLALSLTETAESSELAEAGEFTPWQESPVPESHQIRGF
                     HSVRLWERDLATTGRFLTEALGFERAGEEDGWHRFGVGGGGSGRWVEVRELPGGRLGQ
                     WGTGAIHHVAFRVADDAEQLAVRKQILAAGSQATPVIDRFWFQSVYFREPGGALFE"
     gene            complement(884..3592)
                     /locus_tag="VEL74_16775"
     CDS             complement(884..3592)
                     /locus_tag="VEL74_16775"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HYG64233.1"
                     /translation="MFSKTSVLMIALALGLSLFFQGAAVLPAQTPANPPVTAPATAPV
                     PSLLAPLSSEQRVELPALDERVPSPAAVLGYPLGSRFTHWDRIVTYLQALTAASPRVR
                     LREYGRTYEGRPLHLVVISTPENLARLEEIRQERLRLADPGALSPDERERLSGRVPAV
                     LWLAYGVHGNESSSAEAAMAAAYVLAAAQGETAELLRDLIVIIDPLSNPDGRERYVSA
                     FEQRQGREADPHRAAAEHWEPWPGGRQNHYAIDLNRDWAWMSQVETQQRIAAYRAWEP
                     QVYVDFHEMGSESSYFFPPAADPVHPRIDRRIVSWLDVFGRANAQAFDRQGWIYFKSE
                     NFDLFYPGYGDSYPSLRGAVGMTYEMAGGGRAGLALELPDGSVLTLADRVARHLTTSL
                     ATFRTAGKNSRRLLQDFVANRLQAASSAGPAYLWAGDQQEAGALADLLTSHGVRVERL
                     GQAAEVPVRSLRGERLQEEAAPRRFPAGTYVVSASQPLGTLVRVLMELDSPMAEGFVE
                     RQRERFEQSLDGEFYDITAWSLPLAYNLETWVADTTPRGLGPVPARAGGLRGTGDLGF
                     LVPPGGIASYRLAAELQRRGIRHRVALAGFTAGGTSYPAGTLFVPRHGNPADVRDALS
                     RLLTEAGIEGQAVASSYEVSGLSLGSNDMAPVRPVRVGLLSGAGVDPTSFGSLWHLLD
                     QQVRIPHDRLDTAQLRQVSLRDYDVLLLPSGDYTDAIGERSREALDGWVKGGGVLVAV
                     GDAIQWLRDNKMTEVKKWASPAEEPEGDPESDSEEPRVSAERPINVPGAVVATRLQAR
                     HPLTLGLTAPPAVLVEGTTVLEATGDPRKDVLVATDDPVVAGFAWPEAEERLAGSLLV
                     GAESRGQGAVIVFAQEPAFRLFWRATTPILLNALLYAPSAGLGGRS"
     gene            complement(3618..>4438)
                     /locus_tag="VEL74_16780"
     CDS             complement(3618..>4438)
                     /locus_tag="VEL74_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYG64234.1"
                     /translation="AEGPFITVNSGSLPADLLESNLFGHVRGAFTGAVASKKGLFEMA
                     NGGSIFFDEIGNIPLETQAKLLRVIQEREFMRLGGLDIIRVDVRIIAATNADLEAAVH
                     QGQFREDLFYRLNVITLQLPPLRKRTEDIPLLAQHFLAHYARENEKAIREISPQAMEM
                     LLDYHWPGNVRELENVIERAVVLSSGEVLGVDLLPGSVRNPESVLLPAPNLPANGFSF
                     KDAVSEYERQLIIKALQSSGGVQKRAAELLQVKPTTLHEMMKRLNISPEGVFSS"
BASE COUNT          633 a         1572 c         1625 g          608 t
ORIGIN      
        1 ctcgaagagc gcgccgccgg gctcccggaa gtagaccgac tggaaccaga agcggtcgat
       61 caccggcgtg gcctgggagc cggccgccag gatctgcttc cggaccgcga gctgctccgc
      121 gtcatcggcg acccggaacg ccacgtgatg gatggcgccg gtgccccact gcccgagccg
      181 gcccccgggc agctcgcgca cctcgaccca cctgccggag ccgccgccgc caacgccgaa
      241 gcggtgccag ccgtcctcct ctccggcgcg ctcgaagccg agggcctcgg tgagaaagcg
      301 cccggtggtg gcgaggtctc tctcccagag ccggaccgag tggaagccgc ggatctggtg
      361 gctctccggc accgggctct cctgccaggg ggtgaactcg cctgcctccg ctaattccga
      421 tgattccgcg gtctcggtca gcgacagggc cagcccgtgc acgtcgggga acgccagcac
      481 ccgctcgccg aagcggctcg ccgcgatggc cggcccgact ctcatcgatt ccagccgctc
      541 ttcccagaag gcgaggctcc cctcgggcac ggcgaagccc acttccacgg ccagacccgt
      601 ccccgtgcgg ccggccccca tcgatggcca tgggaagaag gtgaggtcgg tgccggggtg
      661 cccttcggcg tcggcataga agagatggta ggtgtcgggc gcgtcctggt tgaccgagcg
      721 cttgaccagc cgcaggccga gcacctcggt gtagaagtgg aggttctcgc gggggtcgcc
      781 ggcgatggcg gtgacgtgat ggatgccgtg aacggggctc atgaccactc cctccctctc
      841 taccggcggg gcaagaagcg tgccccgccg gatcgtcgga tgctcagctc cggccgccga
      901 gcccggcgct gggagcgtag agcagtgcgt tcagcaggat cggcgtggtg gcccgccaga
      961 agagacggaa cgccggctcc tgggcgaaga cgatgaccgc cccttggccg cgcgactcgg
     1021 cgccgaccag cagcgacccg gccaggcgct cctccgcctc gggccaggcg aagcccgcga
     1081 ccaccgggtc gtcggtggcc accagcacgt ccttgcgcgg atcgcccgtc gcctcgagca
     1141 ccgtcgtccc ctcgaccagc accgcgggcg gcgcggtgag gccgagggtc agcgggtggc
     1201 gggcctggag gcgcgtggcc accacggcgc cggggacgtt gatggggcgt tcggccgaga
     1261 cccgcggctc ctcggagtcg ctctcggggt ccccctccgg ctcctcggcc ggcgacgccc
     1321 acttcttcac ctcggtcatc ttgttgtccc gcagccactg gatggcgtcg cccaccgcca
     1381 ccagcacgcc gccccccttg acccagccgt ccagcgcctc gcggctccgc tcgccgatgg
     1441 cgtcggtgta gtcgccgctg gggaggagca ggacgtcgta gtccctgagg ctcacctggc
     1501 ggagctgcgc cgtgtccagg cggtcgtggg ggatccgcac ctgctggtcg agcagatgcc
     1561 agagggagcc gaaggaggtg gggtccacgc cggcgccgct cagcaggccg acgcgcaccg
     1621 gccgcaccgg cgccatgtcg ttggagccca gggaaaggcc ggagacctcg taggacgagg
     1681 cgaccgcctg cccctcgatg ccggcctcgg tcagcagccg cgacagcgcg tcccgcacgt
     1741 cggccggatt gccgtggcgg gggacgaaca gggtacccgc cgggtagctc gtgccgccgg
     1801 cggtgaagcc ggccagcgcc acccgatggc ggatgccgcg gcgctggagc tccgccgcca
     1861 gccggtacga ggcgatcccc ccggggggca ccaggaagcc gaggtcgccc gtgccgcgca
     1921 ggccaccggc acgggccggc acgggcccca gaccgcgggg ggtcgtgtcg gcgacccagg
     1981 tctcgaggtt gtaggcgagc ggcagcgacc aggccgtgat gtcgtagaac tcgccgtcca
     2041 ggctctgctc gaagcgctcg cgctggcgct cgacgaagcc ctcggccatg ggcgagtcca
     2101 gctccatcag cacccgcacc agcgtgccca gcggctggga ggccgacacc acgtaggtgc
     2161 cggccgggaa gcggcgcggc gccgcctcct cctggaggcg ctccccccgc aggctgcgca
     2221 ccggcacctc ggccgcctgg ccgagccgct ccacccgcac cccgtgggag gtgagcaggt
     2281 cggcgagggc tccggcctcc tgctggtcgc cggcccagag ataggccggc ccggcgctgg
     2341 aagcggcctg caggcggttg gcgacgaagt cctggagcag gcggcggctg ttcttgcccg
     2401 cggtccggaa ggtggccagc gaggtggtga ggtggcgcgc gacgcggtcg gccagcgtca
     2461 gcaccgagcc gtccggcagc tccagggcga gaccggcccg gccgcccccc gccatctcgt
     2521 aggtcatgcc caccgcgccg cgcaggctgg ggtaggagtc gccgtagccg ggatagaaga
     2581 ggtcgaagtt ctccgacttg aaatagatcc acccctggcg gtcgaaggcc tgggcgttgg
     2641 cgcggccgaa gacgtcgagc caggagacga tccgccggtc gatccgcggg tggaccgggt
     2701 cggccgccgg ggggaagaag tagctcgact cgctccccat ctcgtggaag tcgacgtaga
     2761 cctgcggctc ccaggcacgg taggcggcga tgcgctgctg cgtctcgacc tggctcatcc
     2821 aggcccagtc gcggttgagg tcgatggcgt agtggttctg acggccgccc ggccagggct
     2881 cccagtgctc ggccgcggcg cggtgggggt cggcctcccg gccctgccgc tgctcgaagg
     2941 cgctgacgta gcgctcccgc ccgtcggggt tcgacagcgg atcgatgatc acgatcaggt
     3001 cgcgcagcag ctcggcggtc tccccctgcg cggccgccag cacgtaggcc gcggccatgg
     3061 ccgcctcggc ggaggaggac tcgttgccgt gaacgccgta ggcgagccag aggaccgcgg
     3121 gcacgcggcc cgagaggcgc tcgcgctcgt ccggcgacag ggcgccgggg tcggccaggc
     3181 gcaggcgctc ctggcggatc tcttccaggc gcgccaggtt ctccggcgtg gagatgacga
     3241 cgaggtgcag cggcctcccc tcgtaggtgc gcccgtactc ccgcagccgc acccgcggcg
     3301 acgccgcggt cagcgcctgg aggtaggtca cgatccggtc ccagtgggtg aagcggctcc
     3361 ccagggggta gccgagcacc gcggccgggc tggggacccg ttcatcgagc gccggcagct
     3421 ccacccgctg ctccgatgac aacggagcga gcaggctcgg cacgggcgcc gtggccggag
     3481 cggtgaccgg agggttggcc ggcgtctggg ccggcaggac cgccgccccc tggaaaaaga
     3541 gggagaggcc gagggcgagc gcgatcatca agacggacgt cttggaaaac attcagagcc
     3601 tttctcgtgg cccgtagtca ggaagagaag acgccttcgg gcgagatatt gaggcgcttc
     3661 atcatctcgt ggagcgtggt cggcttcacc tggagcagct cggccgcgcg cttctgcacg
     3721 ccgcccgacg actggagcgc cttgatgatg agctggcgct cgtactcgct caccgcgtcc
     3781 ttgaaggaga agccgttggc gggcaggttc ggcgccggca ggagcaccga ctcggggttg
     3841 cgcaccgagc cgggcagcag gtccaccccc agcacctcgc cgctcgacag caccaccgcg
     3901 cgctcgatga cgttctccag ctcgcggacg ttgccgggcc agtgatagtc gaggagcatc
     3961 tccatcgcct gcggcgagat ctcccggatc gccttctcgt tctcccgcgc gtagtgggcg
     4021 aggaagtgct gggcgagcag cgggatgtcc tccgtccgct tgcgcagcgg cgggagctgg
     4081 agggtgatga cgttcagccg gtagaacagg tcttcgcgga actgcccctg gtgcaccgcg
     4141 gcctcgaggt cggcgttggt ggcggcgatg atccgcacgt cgacgcggat gatgtcgagc
     4201 ccgcccaggc gcatgaactc cctctcctgg atcacccgca gcagcttcgc ctgggtctcc
     4261 agggggatgt tgccgatctc gtcgaagaag atggagccgc cgttcgccat ctcgaacagc
     4321 cccttcttgc tcgccaccgc gccggtgaag gcgccgcgca cgtgaccgaa gaggttgctc
     4381 tccaggaggt ccgccggcag cgaccccgag ttgacggtga tgaacggccc ctcggcgc
//
LOCUS       DASPJI010000601         4695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_720708, whole genome shotgun sequence.
ACCESSION   DASPJI010000601 DASPJI010000000
VERSION     DASPJI010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720708"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4695
                     /locus_tag="VEL74_16785"
     CDS             <1..>4695
                     /locus_tag="VEL74_16785"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64235.1"
                     /translation="GQRFYRSGDRVRLLPDGNLEFLGRLDHQVKLRGFRIELGEIEEA
                     LGELEAVREAAVVVREDRPGDRRLVAYVVGDAEPEALRAWLRERMPEYMVPSAFVTLT
                     AFPLTPHGKVDRKALPVPEQPGAESFLAPRTPVEEILAGIWAEVLGVEQVGADGHFFE
                     LGGHSLLATRVMSRLRDSFGVEMPLRDLFEAPRLADLAARIEAARRSGSLASAPPLVP
                     VPRVGTLSLSFAQQRLWFIDQLEPGSPLYNMPVALRVEGSLDAAVLALCLGEIVRRHE
                     ALRTVFAALQGTPVQVIQPAEPFRLPVVDLSALPESVREELARTLAVAEAGWPFDLAQ
                     GPMLRGLLLRLAESDHAVMLTMHHIASDGWSMGVLVREVAALYPAFAAGRPSPLPELP
                     VQYADFAVWQSSWLRGEVLEREIDFWRCQLAGLPPLLELPTDHPRPAAQSYRGAVRPV
                     RLPAGFTRQMEALAREEGATLFMLLLAGFQTLLARLSGQDDVAVGSPVAGRNRSETER
                     LIGFFVNTLVLRGDLTGAPTFRELLGRVRETALAAYLHQDVPFEKLVEELAPERSLAH
                     APLFQVVLVLQNAPVESLVVEDLRLRPVNVAGATAKFDLTINLEEHGGELIGIVEHAT
                     DLFDTTTIDRLFAGFERLLAAAMADAALRVAELPLLSAAERHQLLEWGGIREEFAGAS
                     TLHDRFAEQARRSPDAAALTHGGMTLSYGALHRRSNQLAYWLRRQGAAPESRVGLLLD
                     RSVDLVVGILGVLKAGAAYVPLDPDSPRERLAFMLEDAGIQTVVRAEELALLEKLPAE
                     DLEPLADASSLAYVIYTSGSTGRPKGTLITHGDVVRLFDATDPWFGFGERDVWTLFHS
                     YAFDFSVWEIWGALLYGGRLVIVPWEVSRSPELFLDLLDAERVTVLNQTPSAFVQLAQ
                     AEAQRGTVETSLRFVIFGGEALDPASLEPWFERHGDARPRLVNMYGITETTVHVTYRP
                     LSMADARGEHRSVIGVPIADLSLAVLDPSLVPAPIGVPGELVVGGAGLARGYLGRPEL
                     TAERFVPDSSGGRPGARLYRSGDLGRFLPSGEVEYLGRLDHQVKIRGFRIELGEIEAA
                     LAAVPGARQAVLLVREDRPGDRRLVAYVVGDATVAELRQALRDRLPDYMVPAAFVTLA
                     ALPLTVNGKVNRRVLPAPEQAGSEETYAVLSTPVEEVLAGIWADLLGLDRVGADDHFF
                     DLGGHSLLATQVMSRLRQAFGVEMPLHDLFEAPVLADLAARIEAAQRTGMVPPASSIL
                     PVLREGGLPLSFAQQRLWFIDRVQSGSPMYNIAAALRVEGPLDAAVLARCLGEIVCRH
                     EALRTAFVAPRGTPVQVIQPAEPFPLSMVDLSALSEIAREELARTLAAAEAARPFDLA
                     RGPLLRGVLLRLAESDHAVVLTMHHIASDGWSMGIMVREVAALYPAFAAGHPSPLPAL
                     PVQYADFSVWQRSWLQGGVLEQEIDFWRQRLAGLPPLLELPTDRPRPALLSHRGATRS
                     VHLPAELSRPMEALGRREGATLFMVLLAGFQALLARTSGQDDLAVGTPVAGRNRVEIE
                     GLIGFFVNTLVLR"
BASE COUNT          592 a         1557 c         1698 g          848 t
ORIGIN      
        1 ggtcagcggt tctaccgcag cggcgaccgc gtgcggctcc tgcccgacgg caacctcgag
       61 ttcctcggtc gtctggacca tcaggtcaaa ctgcgcggct tccgcatcga gctgggggag
      121 atcgaggagg cgctgggcga actcgaggct gtacgcgaag cggccgtggt ggtccgggaa
      181 gaccgacccg gtgaccggcg tctggtcgct tacgtggtcg gtgacgcgga gcccgaggcg
      241 ctccgggcgt ggctgcgcga gcggatgccg gagtacatgg tgccctccgc tttcgtgacc
      301 ctcacggcgt tccccctcac gccgcatggc aaggtggacc gcaaggcgct gcccgttccg
      361 gagcagccgg gcgcggagag ctttctggcg ccgcggacgc cggtggagga gatcctcgca
      421 ggcatctggg ccgaggtcct cggagtcgaa caggtcgggg cggacgggca cttcttcgaa
      481 ctgggggggc actccttgct ggcgacgcgg gtgatgtcgc gcctgcgcga ctccttcggc
      541 gtcgagatgc cgctgcgcga tctcttcgag gccccgaggt tggcggatct cgccgcacgg
      601 atcgaggcgg cgcggcggtc cggttcgctg gcctccgctc cgcctctggt gccggtgcca
      661 cgcgtgggga cgctatcctt gtcgttcgcc cagcagcgcc tttggttcat cgatcagctc
      721 gaaccgggca gcccgctcta caacatgccg gtggctctcc gcgtcgaagg gtcgttggac
      781 gccgcggtgc tggcgctctg cctcggcgag atcgtgcgcc gccacgaggc gttgcggacg
      841 gtgttcgccg cgctgcaagg gacgccggtg caggtgattc aaccggccga gcccttccgt
      901 ctccccgtgg tggatctgtc ggcgttgccg gagagcgtgc gcgaggagct ggcgcggacc
      961 ctcgccgtgg cggaggcggg ctggccgttc gacctcgcgc agggcccgat gctgcgcggc
     1021 ttgctgttgc gcctcgccga gagcgatcac gccgtcatgc tgaccatgca ccacatcgcc
     1081 agcgacggct ggtccatggg cgtgctcgtg cgcgaggtcg cggcgctcta tccggctttc
     1141 gccgcgggac gcccgtcgcc tctgccggag ctgccggtgc agtacgcgga cttcgcggta
     1201 tggcagtcct cctggctgcg cggcgaggtt ctggagcggg agatcgactt ctggcgctgt
     1261 cagctcgccg gtctgccgcc gctcttggag ctgcccactg accatccgcg gcccgcagcg
     1321 caaagctacc gcggcgcggt acggcctgtg cggctccccg ccggttttac ccggcagatg
     1381 gaggctctcg cacgcgaaga gggggcgacc ctcttcatgc tgctcctggc cggcttccag
     1441 accctgctgg cgcgtctcag cgggcaggac gatgtcgcgg tgggctcgcc cgtcgccgga
     1501 cgcaaccgga gtgagaccga aagattgatt ggatttttcg tcaacacgct ggtcctgcgc
     1561 ggcgatctga cgggagctcc cacgttccgc gagttgctcg gccgggtgcg tgagaccgcg
     1621 ctggccgcct atctgcacca ggacgtgccg ttcgaaaagc tggtcgagga actggccccg
     1681 gagaggagcc tcgcccacgc cccgctgttc caggtggtac tcgtcctgca gaacgctccg
     1741 gtagaaagcc tggtagtcga ggatctgcgc ctgcgacccg tgaacgtggc aggagcgacg
     1801 gcgaaattcg atctcaccat caacctcgag gagcacggcg gcgagctcat tggcatcgtc
     1861 gagcacgcca cggatctttt cgataccacg accatcgatc ggctgttcgc gggcttcgag
     1921 cggctgctgg ccgcggcgat ggccgatgcg gctttgcgcg tcgcggagct gccgctgctc
     1981 agtgcggcgg agcggcacca gctcctggag tggggaggga tccgggagga gtttgccggg
     2041 gcctcgacgc tccacgatcg tttcgcggag caggctcggc ggtccccgga tgctgcggcc
     2101 cttacccacg gcgggatgac cctgagctac ggcgcgctgc accggcgatc taaccaactg
     2161 gcctactggc tgcgccggca gggcgcagcc cccgagagcc gtgtcggtct gctgctggat
     2221 cgctccgtgg atctggtggt gggcatcctg ggtgtcctca aggccggggc tgcctatgtg
     2281 ccgctcgatc ccgattctcc gcgcgagcgg ctggccttca tgctggagga cgcgggtatc
     2341 cagaccgtgg tgagggccga ggagctcgcg ttgctggaga agttgcctgc cgaggacctc
     2401 gagcctctgg cggacgcatc gagcctggcg tacgtgatct atacctcggg ctccacgggt
     2461 cgacccaagg gcactctgat cacccatggc gatgtcgtcc ggctgttcga cgccaccgac
     2521 ccgtggttcg ggttcggcga gcgggacgtg tggacgctct tccattccta cgccttcgac
     2581 ttttcggtct gggagatctg gggcgcgttg ctctatggcg gccggttggt gatcgtgccc
     2641 tgggaggtga gccgctcgcc cgagctgttc ctggatctgc tggacgccga gcgggtcacg
     2701 gtgctcaacc agaccccctc ggccttcgtc caactcgcgc aggccgaggc gcagcgcggc
     2761 actgtggaga cgtctctgcg gtttgtgatc ttcggtggcg aggcgctcga tccggccagc
     2821 ctggagccgt ggttcgagcg ccatggcgac gcgcggccgc ggctggtcaa tatgtacggc
     2881 atcaccgaga cgacggtgca cgtcacctac cggccgctgt ccatggcgga cgcccggggc
     2941 gagcaccgta gcgtcatcgg cgtgcccatc gccgacctgt ctctggccgt gctggacccg
     3001 agtctggtgc cggcccccat cggcgtgccg ggcgaactgg tggtgggagg cgcggggttg
     3061 gcccgcggct atctgggccg gccagagctg acggcggagc gcttcgttcc ggactcgtct
     3121 ggtggccggc cgggagcgcg gctctaccgg tcgggcgacc tgggacgttt cctccccagc
     3181 ggcgaagtgg agtacctcgg ccggctggat caccaggtga agatccgcgg cttccgcatc
     3241 gagctgggag agatcgaggc ggcgctggca gcggtgcccg gagcccgcca ggcggtcttg
     3301 ctggtgcgcg aggatcgtcc tggcgaccgg cggctggttg cctatgtggt gggcgatgct
     3361 acggtggcgg agctccgcca ggcgttgcgc gatcggctgc cggactacat ggtgccggcc
     3421 gccttcgtga ctcttgcggc gttgcccctc acggtcaacg gcaaggtgaa ccgcagggtg
     3481 cttcccgcgc cggagcaggc gggcagcgag gaaacctatg ctgtgctgag cacgccggtc
     3541 gaagaggtcc tcgccggaat ctgggccgac ctcctcgggc tcgatcgggt cggggcggac
     3601 gatcatttct tcgacctggg cggccattcc ctgctggcca cccaggtgat gtcgcgcctg
     3661 cgccaggctt tcggcgtcga gatgccgctc cacgacctct tcgaggcacc ggtactggcg
     3721 gacctcgccg cccgcatcga ggcggcgcag cgaacgggca tggtcccgcc agcttcgtcc
     3781 atccttcccg tgctgcggga gggtggcctg ccgctgtcgt tcgcccagca gcggctctgg
     3841 tttattgatc gggtccaatc gggcagcccg atgtacaaca tcgcggcggc gctacgtgtc
     3901 gagggtcctc tcgacgctgc ggtgctggcc cgctgccttg gtgagatcgt gtgccgtcac
     3961 gaggccttgc gtacggcctt cgtcgcgccg cggggcacgc cggtgcaagt gattcaaccg
     4021 gctgagcctt tcccgctttc catggtggat ttgtcggcgc tgtcggagat cgcgcgcgaa
     4081 gagctggcac ggacgctcgc tgcggcggag gcggcccggc cgttcgacct cgcgcgcggc
     4141 ccgctgctgc gtggcgtatt gctgcgcctc gccgagagcg atcacgccgt tgtgctgacc
     4201 atgcatcaca tcgccagcga cggctggtcc atgggcatca tggtgcgcga ggtcgcggcg
     4261 ctctatccgg ccttcgctgc gggccaccct tcgccattgc cggcgctgcc ggtgcagtac
     4321 gcggatttct ctgtctggca gcgctcttgg ctgcagggcg gggtgctgga gcaggagatc
     4381 gacttctggc gccaacggct cgccgggcta ccgccgctcc ttgagctgcc taccgaccgc
     4441 ccccggcccg cgctgctcag ccaccgcggc gccacccggt cggtgcatct gccggccgag
     4501 ctctcccggc cgatggaggc tctgggccgg cgcgagggcg cgacgctctt catggtgctc
     4561 cttgccggct tccaagccct gctggcgcgc accagcgggc aggacgatct cgcggtgggc
     4621 acgcctgtgg ccggccgtaa ccgggtggag attgagggac tgatcgggtt tttcgtcaac
     4681 accctggtgt tgcgc
//
LOCUS       DASPJI010000602        11097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_150126, whole genome shotgun sequence.
ACCESSION   DASPJI010000602 DASPJI010000000
VERSION     DASPJI010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11097)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11097
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150126"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VEL74_16790"
     CDS             <1..826
                     /locus_tag="VEL74_16790"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYG64236.1"
                     /translation="TDPEVVRRCVDGVEVVHHVAAAFRELNVPDTYYHEVNVGGTRNV
                     LAAAARAGVRKVVYCSTCGVHGNVDHPPAGEDAPIKPADYYQRTKYEAEPVVQEYFRQ
                     GLKTVILRPAAIYGPGDPERFFLIYRRVAKGTFPMFGNGRTYYHPLYIDNLVDAFVLA
                     MEEGRGEGEAYLIADEHYVEIEDLVRRVGKSLGMDVKVPHFPVWPVVAAGHVCEKICK
                     PFGVTPPLFPRRVDWYRQNRAFKIDKAKRDLNYQPRVGLDEGLRRTAEWYRQEGYL"
     gene            1023..2525
                     /locus_tag="VEL74_16795"
     CDS             1023..2525
                     /locus_tag="VEL74_16795"
                     /inference="COORDINATES: protein motif:HMM:NF027900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HYG64237.1"
                     /translation="MARRVLSKTFLAGLLALTTAAAASALPCYDTAEVTLNSTASYNA
                     NSGSPNPFDLSVVARVTSPSGRAYSVDGFFDGNGAGGAIGKVFKVRIYADEVGTWRWT
                     VASTNVPGLAGKSGSFAVSGVLPGVFGKGPVVENPARPRTFMYQHGTPVFLVDKFLDN
                     AAPNPIKYSHTLLSEKLTDTNRAARLARHTGMKLNKMNVYYANRGDYASMSTTPWVGT
                     ATANDKTRFDLARWRMYDQWIVKLRNAGLVAHLWFFADDSAFGDLPDAHRQRLIKYAM
                     ARHSAYVHTKYILALEWQEGWTTTEVNNHANFIQAKNPWARMVTVHGVTGDFKFPGAA
                     WADYMDIQAGNSASHSVVHSRGLTNRNLAAKPLLQEEHGLGEETTINRQRAWAAFAGG
                     AAGVGTGAFLQHLATFANQIRFERMEPNQALVRSGGAYALAEKGVAYVFYLHTGGTVG
                     VDLSGVTGTFVAQWYDPRTGAYRAAPAASGGGVRTYTAPAAGDWVLYLRK"
     gene            2677..3960
                     /locus_tag="VEL74_16800"
     CDS             2677..3960
                     /locus_tag="VEL74_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64238.1"
                     /translation="MRFRPWVWSVLLSSALASSSSLEAAPLHPAYDLQGPGLSLAHAG
                     IGLAGLAAGTRTLTLDVGGPVELALLYWAGRERPCQLDAVRSECSVAAEPYLDQVLRF
                     DGARVTGTVTGTETQPTSNLGAILNIGYMADVTDAVRARGMGRLSFALADGDPLSNLA
                     ELDGAGLLVVYADPARPAARVLVSHGLDFGYGEDWTPGETQVTDPFTFLHGAARQPRS
                     GEVVLFVGNATRQRPDRIEITNAANIAALANTLDGSAGGQWDADRIPVEVPGGAISTS
                     LQVFSEPYGRNPDSLLWVMAALRVPLPVPTSCSTAFWGDHPEAWRETGIATSQRVRST
                     FSQAAGYGDVADATLRNALRFRSSSDLLGAAKELLREATASLLNAVHPALESPRTRTQ
                     LINQVNEALRSRNLPAIWELTGQLERENAAGCPLR"
     gene            complement(3964..4986)
                     /locus_tag="VEL74_16805"
     CDS             complement(3964..4986)
                     /locus_tag="VEL74_16805"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HYG64239.1"
                     /translation="MPYNVVHRQGIDRATAANRRPEESLQTPAGPECAFIVGHRKSGS
                     TWLLNLLSLHPDVRGVMETHLFRHGWQETDPASRVDKLFEQTPWGAGGPRQWPVHQMK
                     RLAKPFLLRVKPALRLAPDERPAELPDLGLLDQLALRRELRRIGTPEEFVQRFFEILL
                     ERLRPRRYLIEKSPRNVYFVERIRSLFPRAKLIVIYRDGRDVVVSDRFFTEDYGNRSF
                     SFEQAVADWRRDMETHLRYAEGHTLCAVSYEALLTDGKPVVESLLRFLGLPYDDALVA
                     DLLERSSFRFHAGRERGQENRKRFYRKGIVGDWRNHFTPEHKRIFKDVAGDMLIRLGY
                     EKDLDW"
     gene            complement(5036..7063)
                     /locus_tag="VEL74_16810"
     CDS             complement(5036..7063)
                     /locus_tag="VEL74_16810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYG64240.1"
                     /translation="MSTESRGSSFSSTTIPSTFGGAPRRDRAPVAGATGEQAGEQAGT
                     RSRAVTLTLAAILLLATGIRFWGVLHDLPFSYYGDELHMMKRAMAMGTGDLNPHWFHK
                     PALLMYVLAFFYGLYFAAGLLTGRFESTEEFGAHFLFEHGPFLLIGRLVVLAFGVATV
                     YVVYRLGRKAFGHPAAGLAGALVAAVLAPMISSSQEIKSDVPCGFLMALSVYLYLGTR
                     ETGRLRPLVLASLLAGAAMGTHYYGIVLVPTFIAMEALRALSPAVPPVRWRSLLVRGS
                     LVGLLFLGGFFLTSPFNFLDPTWARQTFGGVIGSLGLSSVGDAQDAGESAEGKPVRYE
                     PDTGTAYEPGLAAWAGASAAFFRVITSQPSMGLALTLLSLLGLAATLARRETRWYGLL
                     VLIPCLFFFLAAITVAAYHAQPRHFNAIYPLLATMVWPGLLVLTAPLRSAPRRAWAVA
                     GVLAVLACLPTLAESVRHNREINRLDSRLVSYRWLVSNMRDDTRILVDEYGPLLNPNP
                     QASQRLAERLERLPRGPFTHHQGLRVRLLQQYPPADGVNLDEFGHQWWLPQEKTDAEL
                     LNPVDMDMANPLISREPKPLDAYRAQGIRYLVTNSEAQGRYRKRQLQESFPSFARFYR
                     ELRGAHLIKTFDPATWGGKGPVVWVYDLAPAANDNEAVPENRQDVSARRAG"
     gene            complement(7080..8108)
                     /locus_tag="VEL74_16815"
     CDS             complement(7080..8108)
                     /locus_tag="VEL74_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64241.1"
                     /translation="MRDAMNAQRGALWVVGGEQRGGFTIPEEWHRFRTAVVVRVEGDR
                     AERVLEYQSPPENCPDDRPSFVFKAGTFEGDTAWLCTQTEVLECALPGFAIRRVISLP
                     CFNDLHHVTPTPEGTLLVAVTGLDAVAEVSRDGELIRLTHVLGGDVWDRFSPDVDYRK
                     VPTTKPHHAHPNYVFFVDGEPWVTRFHQRDAVPLHRAGDPAKNGGGERFHVEIQGVHD
                     GCVHDGRLLFTTIDGHVVGFDLATGEKSDLDLNGLGQPDDDRALGWCRGLLPCDGRRA
                     WVGFTRFRYTKLRQNLSWVRHGFKMSDDHRNHPTRVALYDLERPALLHQVDLEDVGMG
                     AVFSIHQA"
     gene            complement(8105..9541)
                     /locus_tag="VEL74_16820"
     CDS             complement(8105..9541)
                     /locus_tag="VEL74_16820"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HYG64242.1"
                     /translation="MIPPAAETLAPAVPPEPVKAPAAAGGGMSLARLRHGGLLFGASQ
                     MLAMVLGFAGTMVLTRAADTAAVASYLLLVQSTITVGLVLQLGLAQATLRFVPVSRGE
                     GGRAATSLLRRRLLGLQVGMWLLAAPILFLVWPGVAWRLDAPELSYATAFLIGIPVLA
                     SLGQLMDSYLRAFRMYSVSAPLTQVLPRVLVLAGFLTLWLAMPGSATWEMLASVYTLS
                     LVLTLLGYAAAMRATTPAEESEPRTAQTPPGVRTILATTTAMGLRSAASVLFVSSDLW
                     ILSWARPHEEVAIYGVASRLIQVMAALPGIASFLLPQEFAVLYADGRKEEMERLARQA
                     STAMAMISVAAFIGLLLLGKPLLGVAFDEVYARGWTVVLILAVGTFWDTASGASGFAL
                     QMTGHHVRLLVLTAGAAILNVILSVLLVPLWGGHGIALSTAVTLIALNTSMVWSARKL
                     VGVRTFIYLRPAEWKQVIQKLIRREREV"
     gene            complement(9668..>11097)
                     /locus_tag="VEL74_16825"
     CDS             complement(9668..>11097)
                     /locus_tag="VEL74_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64243.1"
                     /translation="PWPEAAWLAASEAVPEFPAGPLPKALAGYDEVVSGVLGTTGKAI
                     LDEGITGLMTFGVYPRYWGRWKSPELQCKGKEPPGDRWDDVFWCGTWTDYHNTTATAV
                     LRAMRTGEVQWLDEIAFPGAVRTLHTQIMQCGPDEAWFYCGQSPAGYGGYRSDFNSSH
                     AYFENLYLYYWLTGDSLVVDTLRQGGETMRRHMCETRGPAPVKGAQAQDGPGGPACAA
                     DHPLGKASFTGRVGGQWLAAFHFLGLASDDPSFLEDFKSGLARAVTLQYAELEKDGRR
                     YGFLGGKVGNGPGGSAQAGPLWTNGFYDAENLYRLMVDTGDAPLGKPAVRPSQVLAAV
                     ARTLAGIDSGELAVADAKGGEKGTGQGEPGKKREKDAWPRLLDYRWEGPRVGGRLAGV
                     TAKDRDLYGPEKAGTAALLVRAGRLTGDPGLLKAGEERTRAAIEASRGEAVPLGKLQG
                     QYLNRLHAAVAGLAAGPIGPAKPAR"
BASE COUNT         1713 a         3822 c         3964 g         1598 t
ORIGIN      
        1 caccgatccg gaggtggtgc ggcgctgtgt cgacggcgtc gaggtcgttc accacgtggc
       61 cgcggccttc cgcgagctca acgtccccga cacctactac cacgaggtca acgtcggcgg
      121 cacccgcaac gtcctcgcgg ccgcggcccg ggccggcgtg cgcaaggtcg tctactgcag
      181 cacctgcggc gtccacggca acgtcgacca cccgccggcg ggggaggacg ccccgatcaa
      241 gcccgccgac tactaccagc gcaccaagta cgaggccgag cccgtcgtcc aggaatattt
      301 ccggcagggg ttgaagaccg tcatcctacg cccggcggcg atctacggac cgggggatcc
      361 ggagcgcttc ttcctgatct accggcgcgt cgccaagggg accttcccca tgttcggcaa
      421 cgggcgcacc tactatcacc cgctctacat cgacaacctg gtcgacgcct tcgtcctcgc
      481 gatggaggag gggcggggcg agggggaggc ctacctcatc gccgacgagc attacgtcga
      541 gatcgaggat ctggtgcggc gggtcggcaa gtccctcggg atggacgtca aggtccctca
      601 cttcccggtc tggcccgtgg tggccgccgg ccacgtgtgc gagaagatct gcaagccttt
      661 cggcgtcacg ccgccgctct tcccccgccg cgtcgactgg taccgccaga accgggcgtt
      721 caagatcgac aaggcgaagc gagatctcaa ttatcagccg cgcgtgggcc tcgacgaagg
      781 gctgcgccgg acggctgaat ggtaccggca ggaaggctat ctttgaaaga tcgcaagtca
      841 ctgatttcaa agtgcaaagc ggaggtactc tggatttaga atcaggattg taaatatttc
      901 cttgaaattt tggcgtttgg atgctagttt gcaccggcag tcagggaagt aagcgggtag
      961 cttgctccag gaacttactg gacccgcgcc aagggcgcct agttgtggag accacgacgc
     1021 cgatggctag acgtgttctt tcgaagacct ttctcgcagg gctcctcgcc ctcaccaccg
     1081 ccgccgccgc ttccgccctc ccgtgctatg acacggcgga agtcaccctc aactccacgg
     1141 cctcctataa cgccaactcg ggaagcccca accccttcga cctgtccgtg gtcgcccgcg
     1201 tgacgtcgcc ttcgggccgc gcctactcgg tggatggctt cttcgacggc aacggagccg
     1261 gcggcgcgat cggcaaagtc ttcaaggtga ggatctacgc tgacgaggtg ggcacctggc
     1321 gctggaccgt tgccagcacc aacgtgccgg gtctcgcggg aaagagcggc agcttcgccg
     1381 tctcgggcgt ccttcccggc gtcttcggca agggtccggt ggtggagaac ccggcccggc
     1441 cgcggacctt catgtaccag cacggcaccc cggtcttcct ggtcgacaag ttcctcgaca
     1501 acgcggcgcc caaccccatc aagtactccc acacgctgct gtcggagaag ctcaccgaca
     1561 ccaaccgcgc ggcgcggctc gcccgtcaca ccgggatgaa gctcaacaag atgaatgtct
     1621 actacgccaa ccgcggcgac tacgcgagca tgtcgaccac cccctgggtg gggacggcca
     1681 cggccaacga caagacccgc ttcgacctcg cccgctggcg catgtacgac cagtggatcg
     1741 tcaagctgcg caacgccggg ctggtggccc acctctggtt cttcgccgac gactcggcct
     1801 tcggtgacct tcccgacgcc caccggcagc ggctcatcaa gtacgccatg gcccgccact
     1861 ccgcctacgt tcacaccaag tacatcctgg ccctggagtg gcaggagggg tggaccacca
     1921 ccgaggtgaa caaccacgcc aacttcatcc aggccaagaa cccgtgggcg cggatggtca
     1981 cggtgcacgg cgtcaccggc gacttcaagt tccccggcgc cgcctgggcc gactacatgg
     2041 acatccaggc gggcaacagc gccagccact cggtggtcca cagccgcggc ctgaccaacc
     2101 ggaacctggc cgccaagccg ctcctccagg aggagcacgg cctgggcgag gagacgacca
     2161 tcaaccgcca gcgggcgtgg gcggcgttcg ccggcggcgc cgcgggtgtc ggcaccggcg
     2221 ccttcctcca gcatctggcg acgttcgcca accagatccg cttcgagcgc atggagccca
     2281 accaggcgct ggtgcgctcc ggcggcgcct atgccctggc cgagaagggg gtcgcctacg
     2341 tcttctacct gcacaccggc ggcacggtgg gcgtcgacct ctcgggtgtg accggcacct
     2401 ttgtcgccca gtggtacgac ccgcgcaccg gcgcttaccg ggcggcaccg gccgcgagcg
     2461 gcggcggtgt ccgtacctac acggcgccgg ccgccggcga ctgggtgctc tacctccgca
     2521 agtagagggg cgatccgccc tcggcagaac gacggccgcc ggctcccggc ggccgtcgct
     2581 gtttttgtgg tgtggacatg tgctcacgtt gggggcgatt catccttccg gcatgcccat
     2641 tgcttgagca ttcggcattc acctcggagg tgatccatgc gttttcgtcc ctgggtctgg
     2701 tccgtcctcc tctcgtccgc attggcttcg tcttccagcc tggaagcggc cccgctgcac
     2761 ccggcctatg acctccaagg ccccggtctg tccctggcgc acgccggtat cgggctggcg
     2821 ggtctcgccg ccggaacgcg gacgctcacc ctcgacgtgg gggggccggt ggagctggcg
     2881 ctgctgtact gggccggccg ggagcgcccg tgccagctcg acgcggtgcg gagcgagtgc
     2941 agcgtggccg cggagccgta tctggaccag gtcctgcggt tcgacggcgc gcgcgtgacc
     3001 ggcaccgtga ccggaacgga gacccagccc accagcaacc tcggggcgat cctcaacatc
     3061 ggctacatgg cggacgtgac ggacgcggtg cgggcccggg ggatggggcg cctgtcgttc
     3121 gccctggccg acggcgaccc gctgagcaac ctggcggagc tcgacggggc cgggctgctg
     3181 gtcgtctacg ccgatccggc gcggccggcg gcccgggtcc tcgtctccca cgggctggat
     3241 tttggctacg gggaggactg gacgccgggc gagacgcagg tcaccgatcc cttcaccttc
     3301 ctccacggcg ccgcccggca gccccgctcc ggcgaggtgg tgctgttcgt ggggaacgcg
     3361 acgcgccagc ggcccgacag gatcgagatc acgaatgccg ccaacatcgc cgctctcgcc
     3421 aacaccctcg acggttcggc cgggggccag tgggacgccg accgcatccc ggtcgaggtc
     3481 cccggggggg cgatctccac ttcgctccag gtcttctcgg agccctacgg caggaacccc
     3541 gattcgctgc tctgggtgat ggccgccctg cgagtgccgc tgcccgtacc caccagttgc
     3601 tctacggcgt tctgggggga tcacccggag gcctggcggg agacggggat cgcgacctct
     3661 caacgggtga ggagcacctt cagccaggcg gccggctacg gcgacgtggc ggacgccacg
     3721 ctacgcaatg ccctgcgctt ccgttcgtcg tccgatctcc tgggcgcggc caaggagctg
     3781 ctgcgggagg cgacggcctc gctgctcaac gccgtgcacc ccgctctgga gtcgccgcgg
     3841 acgcggacgc agctcatcaa ccaggtgaac gaggcgttgc ggagccggaa tctccccgcg
     3901 atctgggagc tgaccggcca gctcgagcgc gagaacgccg cgggctgccc gctgcggtag
     3961 gcctcaccag tcgagatcct tctcgtaccc gagacggatc agcatgtcgc cggccacgtc
     4021 cttgaagatc cgcttgtgct cgggcgtgaa gtgattgcgc caatcgccca cgatcccttt
     4081 gcggtagaag cgcttgcggt tctcctggcc gcgctcgcgc ccggcgtgga agcggaacga
     4141 cgagcgctcc agcaggtcgg ccaccagggc gtcgtcgtac ggcaggccga ggaagcggag
     4201 caggctttcg accaccggct tgccgtcggt gagcagcgcc tcgtaggaga cggcgcagag
     4261 ggtgtgcccc tcggcgtagc ggaggtgggt ctccatgtcg cggcgccagt cggccaccgc
     4321 ctgctcgaag gagaacgagc ggttgccgta gtcctcggtg aagaagcggt ccgagaccac
     4381 cacgtcgcgg ccgtcgcgat agatcacgat cagcttggcg cgagggaaga gggagcggat
     4441 ccgctccacg aagtagacgt tgcgcggcga cttctcgatc aggtagcggc gcgggcgcag
     4501 ccgctccagc aggatctcga agaagcgctg gacgaactcc tcgggcgtcc cgatccgccg
     4561 cagctcgcgc cgcagggcga gctggtccag gagccccagg tccggcagct cggcgggccg
     4621 ctcgtcgggg gcgagccgca gggcgggctt cacccgcagc aggaaaggct tggccaggcg
     4681 cttcatctgg tggacgggcc actggcgcgg tccccccgcg ccccacggcg tctgctcgaa
     4741 gagcttgtcg acccggctcg ccgggtccgt ctcctgccag ccgtggcgga agaggtgggt
     4801 ctccatgacc ccccggacgt cggggtggag gctcaggagg ttgaggagcc acgtgctccc
     4861 gctcttccgg tgccccacga tgaaggcgca ctcgggtcct gccggcgtct gtagcgactc
     4921 ctcggggcgg cggttcgccg ccgtggctct gtcgattcct tgtcgatgca ctacgttgta
     4981 cggcactcca cacctcccgg aaaacagcga ttgatcgatg tctggcgggc cgggtctagc
     5041 cggcgcgccg ggcggaaacg tcctggcggt tctccgggac cgcctcgttg tcgttggccg
     5101 cgggggccaa gtcgtagacc cagaccaccg gccccttgcc cccccaggtg gccgggtcga
     5161 aggtcttgat cagatgggcc ccccggagct cgcggtagaa gcgggcgaac gacgggaagc
     5221 tctcctggag ctgccgcttg cggtagcgcc cctgggcctc gctgttggtc accaggtagc
     5281 ggatgccctg ggcgcggtag gcgtccagag gcttgggctc ccgcgagatc agcgggttgg
     5341 ccatgtccat gtcgacggga ttgagcagct ccgcgtcggt cttctcctgc ggcagccacc
     5401 actggtggcc gaactcgtcg aggttgacgc cgtcggccgg gggatattgc tggaggagcc
     5461 gcacgcgcag cccctggtgg tgggtgaacg ggccgcgggg cagccgctcc aggcgctccg
     5521 ccaggcgctg ggaggcctgc gggttcgggt tgaggagcgg accgtactcg tccaccagga
     5581 tgcgcgtgtc gtcgcgcatg ttggagacga gccagcggta ggagaccagc cggctgtcca
     5641 ggcggttgat ctcccggttg tggcgcacgc tctcggcgag cgtcgggagg caggccagca
     5701 cggccagcac cccggccacc gcccaggccc tgcgcggggc cgagcgcagc ggcgccgtca
     5761 gcaccagcag ccccggccac accatcgtcg ccagcagcgg atagatggcg ttgaagtgcc
     5821 gcggctgggc gtggtacgcg gcgacggtga tcgccgccag gaagaagaag aggcagggaa
     5881 tcaggacgag cagcccgtac cagcgcgtct cccgccgcgc cagcgtggcg gcgagcccca
     5941 ggagggagag gagcgtcagc gccaggccca tggagggctg cgaggtgatc acccggaaga
     6001 aggccgcgga ggcgccggcc caggcggcga ggcccggctc gtacgccgtc cccgtgtccg
     6061 gctcgtagcg caccggcttc ccctccgcgc tctcgccggc gtcctgggcg tcgcccaccg
     6121 aagagaggcc caggctgccg atgacgccgc cgaaggtctg ccgcgcccag gtggggtcga
     6181 ggaaattgaa cggcgaggtg aggaagaagc cgccgaggaa caggaggccc accagggagc
     6241 cgcgcaccag gaggctccgc cagcgcaccg gcggcaccgc cggcgatagt gcgcgcagcg
     6301 cctccatggc gatgaaagtg gggaccagca cgatcccgta gtagtgcgtg cccatggccg
     6361 cgccggccag gagcgaggcg aggaccagcg gccgcaggcg gccggtctcc cgcgtcccca
     6421 ggtagaggta gaccgacagc gccatcagga agccgcaggg cacgtcgctc ttgatctcct
     6481 gcgacgagct gatcatcggt gcgagcaccg ccgccaccag cgctccggcg aggccggcgg
     6541 cggggtggcc gaacgccttg cggccgagcc ggtagaccac gtagaccgtg gccacgccga
     6601 aggccaggac caccagccgg ccgatgagga ggaagggccc gtgctcgaac aggaaatggg
     6661 cgccgaactc ctcggtcgac tcgaagcggc cggtgagcag gccggcggcg aagtagagcc
     6721 cgtagaagaa ggcgagcacg tacatcagca gcgccggctt gtggaaccag tgcgggttga
     6781 ggtcgcccgt ccccatggcc atggcgcgct tcatcatgtg cagctcgtcg ccgtagtagg
     6841 agaaggggag gtcgtgcaga acgccccaga agcggatgcc cgtggccagc agcaggatcg
     6901 ccgcgagcgt cagggtcacg gcgcgggacc gcgtcccggc ctgctcgccg gcctgttcgc
     6961 cggtagcgcc ggccacggga gcccggtccc gccggggagc cccgccgaag gtcgacggaa
     7021 tcgtggtgga cgaaaacgag ctgccccggg actccgtgct catactgacc tcactcgagt
     7081 catgcctggt gaatgctgaa caccgccccc atccccacgt cctccagatc gacctggtgg
     7141 agcagcgccg gccgctccaa gtcgtagagg gcgacgcggg tcgggtggtt gcggtggtcg
     7201 tcgctcatct tgaagccgtg ccgcacccac gagaggttct ggcgcagctt ggtgtaccgg
     7261 aagcgggtga agccgaccca ggcgcggcgg ccgtcgcacg gcagcaggcc gcggcaccag
     7321 cccagggcgc ggtcgtcgtc gggctggccc agcccgttga ggtcgaggtc gctcttctcc
     7381 cccgtcgcca ggtcgaagcc gaccacgtgg ccgtcgatgg tggtgaagag caggcgcccg
     7441 tcgtgcacgc agccgtcgtg gaccccctgg atctcgacgt ggaagcgctc gccgccgccg
     7501 ttcttggcgg gatcgccggc gcggtggaga ggcacggcgt cgcgctggtg gaagcgggtc
     7561 acccacggct cgccgtcgac gaagaagacg tagttcgggt gcgcgtggtg cggcttggtc
     7621 gtcggcacct tgcggtagtc gacgtcgggg gagaagcggt cccagacgtc accgccgagg
     7681 acgtgggtca ggcggatcag ctcgccgtcc cggctcacct cggccaccgc gtcgagcccg
     7741 gtgacggcga cgagcagggt gccctcgggc gtgggcgtga cgtggtgcag gtcgttgaag
     7801 caggggagcg agatcacccg gcggatggcg aagcccggca gcgcgcactc cagcacctcg
     7861 gtctgggtgc agagccaggc ggtgtccccc tcgaaggtgc cggccttgaa cacgaacgac
     7921 ggccggtcgt ccgggcagtt ctcgggcggc gactggtact cgaggaccct ctccgcccgg
     7981 tccccctcca cccgcaccac gaccgcggtg cggaaccggt gccactcctc cgggatggtg
     8041 aagcccccgc gctgctcccc cccgacgacc cacagcgcac cccgctgggc attcatcgcg
     8101 tctctcaaac ctctctctcc ctccggatca gcttctggat aacctgcttc cactcggccg
     8161 ggcgcagata gatgaaggtg cgcaccccga ccagcttcct ggccgaccac accatggacg
     8221 tgttcagggc gatcagcgtg accgcggtgg acagcgcgat cccatggccg ccccagaggg
     8281 gcaccaggag cacgctcagg atcacgttga ggatggcggc gccggcggtc agcaccagca
     8341 ggcggacatg gtgcccggtc atctgcaggg cgaagcccga ggcgcccgac gcggtgtccc
     8401 agaacgtgcc gacggcgagg atcagcacga cggtccagcc gcgggcgtag acctcgtcga
     8461 aggcgacccc gagcagcggc ttgccgagca ggaggaggcc gataaaggcg gcgaccgaga
     8521 tcatcgccat ggcggtggag gcctggcgcg ccaggcgctc catctcctcc tttcggccgt
     8581 cggcgtagag caccgcgaac tcctgcggga ggaggaagct ggcgatgccg ggcagggccg
     8641 ccatgacctg gatcaggcgg ctcgcgacgc cgtagatcgc cacctcctcg tgcggccggg
     8701 cccaggagag gatccacagg tccgacgaca cgaagagcac cgacgcggcg ctgcgcagcc
     8761 ccatggccgt ggtggtggcg aggatggtgc ggacgcccgg cggcgtctgc gcggtgcgcg
     8821 gctccgactc ctcggcgggc gtggtcgccc gcatggcggc ggcatagccg aggagggtca
     8881 ggaccagcga cagcgtgtag acggaggcca gcatctccca ggtcgcgctg cccggcatgg
     8941 cgagccagag ggtcaggaag ccggccagca ccagcacccg cggcagcacc tgggtgagcg
     9001 gcgccgacac cgaatacatg cggaaggcgc gcaggtagct gtccatgagc tgcccgagcg
     9061 aggcgagcac cgggatgccg atgaggaacg cggtggcgta ggagagctcc ggagcgtcca
     9121 ggcgccaggc gacgccgggc cagaccagga acaggatcgg cgcggccagc agccacatcc
     9181 caacctggag ccccagcagg cgccggcgca gcagcgacgt ggccgcccgc cccccctcgc
     9241 cgcggctgac gggcacgaag cggagcgtcg cctgggcgag gccgagctgc agcaccagcc
     9301 ccaccgtgat ggtggactgg acgagcaaca gataggaggc gaccgcggcc gtgtccgcgg
     9361 cccgggtgag caccatggtc ccggcgaagc cgagcaccat ggcgagcatc tggctggcgc
     9421 cgaagagcag ccctccgtgc cgcaggcgcg ccagggacat gccgccgccg gcggcggccg
     9481 gagccttcac cggttcgggc ggcacggcgg gcgcgagagt ctcggcggca ggcggaatca
     9541 cggggtgatc ccctctctgc tccgctcctg tcccggggcg ttgtgatcgt ccattcgaac
     9601 ctttgcgtcg agaatgcacg gaccttgaac caatgcgccg ggagaggctg caagatcgtt
     9661 gccggggtca gcgggccggc ttggccggtc cgatggggcc ggcggccaaa ccggcgaccg
     9721 cggcgtggag gcgattgagg tattgcccct ggagcttgcc gagggggacc gcctcgccgc
     9781 gggaggcctc gatggcggcg cgggtcctct cctcgccggc cttgaggagc cctggatcgc
     9841 cggtcaggcg gccggcgcgg accaggaggg cggcggtgcc ggccttctcg gggccataga
     9901 ggtcgcggtc cttggcggtg acgccggcca gccggccgcc gacccgcggc ccctcccacc
     9961 gatagtcgag gagccgcggc caggcatcct tttccctttt cttgcccggc tctccctgcc
    10021 cggtaccctt ttctccaccc ttcgcgtccg ccaccgcgag ctcgccgctg tcgatccccg
    10081 ccagggtccg ggcgacggcg gcgagcacct ggctgggacg caccgcgggt ttccccaggg
    10141 gggcgtcgcc ggtgtcgacc atgagccggt acaggttctc ggcgtcgtag aagccgttgg
    10201 tccagagggg ccccgcctgg gcgctgccgc cggggccgtt cccgaccttc ccgccgagga
    10261 agccgtagcg cctgccgtcc ttctcgagct cggcgtactg cagggtgacg gcgcgggcga
    10321 ggcccgactt gaagtcctcg aggaagctgg ggtcgtcgct ggcgagcccc aggaaatgga
    10381 aggcggcgag ccactggccg ccgacgcggc cggtgaagct ggccttcccg agcgggtggt
    10441 cggcggcgca ggcggggccg ccgggaccgt cctgggcctg ggcgcccttg accggcgcgg
    10501 ggccgcgggt ctcgcacatg tggcgccgca tggtctcgcc cccctgccgc agggtgtcga
    10561 ccaccagcga gtcgccggtg agccagtaat agaggtagag gttctcgaag taggcgtggg
    10621 agctgttgaa gtcggaccgg tagccgccgt agccggcggg ggactggccg caatagaacc
    10681 aggcctcgtc ggggccgcac tgcatgatct gggtgtggag ggtgcggacg gcgccgggaa
    10741 aggcgatctc gtcgagccac tgcacctcgc cggtgcgcat ggcgcggagg accgccgtgg
    10801 ccgtggtgtt gtggtaatcc gtccaggtgc cgcaccagaa cacgtcgtcc caccggtcgc
    10861 ccggcggctc cttccccttg cactgcagct cgggggactt ccagcgcccc cagtagcgtg
    10921 ggtagacgcc gaaggtcatg aggccggtga tcccctcgtc caggatcgcc ttcccggtgg
    10981 tccccagcac gccggagacc acctcgtcgt agccggcgag cgccttgggg aggggcccgg
    11041 cggggaactc ggggaccgcc tcggaggcgg cgagccaggc ggcctcgggc caggggc
//
LOCUS       DASPJI010000603         5907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_336016, whole genome shotgun sequence.
ACCESSION   DASPJI010000603 DASPJI010000000
VERSION     DASPJI010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336016"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1858
                     /locus_tag="VEL74_16830"
     CDS             <1..1858
                     /locus_tag="VEL74_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HYG64244.1"
                     /translation="YNDHTAGKGDLFLMVQGQDLQPQLREPKAIRPRPLDRALREKLE
                     ATIAANVRAIRRSPLRVAYALDDEVSWSVLSRPIPWRIHGDDGDYRRWLHSVYGRGAP
                     GVQIVGPDFALEQLGRPLGRLDFSPFLDRMSYNDSVWANLLGALVERCHREDPSTPCG
                     FVGGQAPGLWGGYDYAKIARKVQFIEAYDLGSSAEILRSFAPDIPRVTTHFHKDQRSP
                     GNDRYLAWRYFAHGHRGMIGWVDESWFEGGRPRPWLDRFRPALRELGGVQGPKMVGAR
                     PLDDGIALYYSHPSIQVSWILDAEAHGKTWPNRNEDHRLGTSHNVRRAWTWLLEDAGL
                     QYRFLAYDQVVTGGVPADVRVLILPACYALSDVESRHIAGFAAAGGTVIADFAPGLFD
                     QHGKGRLRGALDDLFGVRHDGTETRADFFGGRLWVETDQDAGYSFRRYRELLETVRPR
                     LHQGYAIAERNLPVGTARQVGKGRAVYLNLSPQRYLMEREERTATEAHRRVFLEPVFA
                     AGVAPWIRAKADRAIPLEVTYWSKQGRTLVFVIQNVPVSGSSTGGGGAEGLVEAVVPV
                     EVRLSMPVRDVRDERTGRKLPDGDRFTFQLDTTEAVFFSFAGEPPTTPPAGS"
     gene            complement(1812..2504)
                     /locus_tag="VEL74_16835"
     CDS             complement(1812..2504)
                     /locus_tag="VEL74_16835"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HYG64245.1"
                     /translation="MSIALIADAHLGGPGGSSEPMIRQLRALPGEGCTRLVLMGDIFQ
                     AWIGDPRYETAEIAAVVPVLRELRRQGLRVDYVEGNRDFFLTGSPYADAFDAIGVEFA
                     FEEDGVRYLAVHGDGLNDKDRQYLFWRWLSKSAPVRLAVLNLPRGLARRMVSSTEQRL
                     SQTNFKHKAALPEEAIRAYAERRLAEGHDVLLLGHFHEPRVWQVKGGEVRLLNAWFRS
                     RRVEWLAALPRS"
     gene            complement(3542..4633)
                     /locus_tag="VEL74_16840"
     CDS             complement(3542..4633)
                     /locus_tag="VEL74_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HYG64246.1"
                     /translation="MANDVESRVWDALKGVTFPGMRRDIVSFGFVHQVKVSGGVATVD
                     LVISTQNEAAAGQVREAAERAVAAIPEISAVQVNLNVQRPASREETAQRAIAQDSSLI
                     PEVRHVVAVASGKGGVGKSTVAANLAIMLGMLGHRVGLLDADIYGPSVPMMFGITEKP
                     RVVGNRIFPFEKYGIRVMSLGFILETDTPVIWRGPMVMRAIEQMLGDVEWGALDYLIL
                     DLPPGTGDAQLTVTQKVPLSGAVIVSTPQDVALIDARKGLAMFRKVNVPVIGIIENMS
                     TFICPHCGEATDIFKKDGGKRTADLLETAFLGSIPLDAKIVEGGDAGVPIVVADPRGP
                     HAGAFRQVAESVVAEVARQAAAKPKLTIV"
     gene            complement(4713..5195)
                     /locus_tag="VEL74_16845"
     CDS             complement(4713..5195)
                     /locus_tag="VEL74_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002757194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HYG64247.1"
                     /translation="MPEVIVNTSPLQYLFQLDLLDLLPELYGDVVVPGGVAREIAAGL
                     DRGVSLPSLSSLPWLEIREVMSPSVLPLVTDLGTGEREVLALALQSTGSLVVLDDFLA
                     RRFADRLRLRMTGTLGILLKARRTGRVDRLAPLLDRLERLNFRLDAGTRANVLRLAGE
                     "
     gene            complement(5188..5439)
                     /locus_tag="VEL74_16850"
     CDS             complement(5188..5439)
                     /locus_tag="VEL74_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002739985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HYG64248.1"
                     /translation="MSRILLDIPDDSLLALKVSADEMGEELRLAAAVKLYELGKLSSG
                     AAARLAGIPKALFLTKLGDYGVPTFRLTEEELRRDLENA"
     gene            complement(5442..>5907)
                     /locus_tag="VEL74_16855"
     CDS             complement(5442..>5907)
                     /locus_tag="VEL74_16855"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYG64249.1"
                     /translation="SLFQIPVFGWAMKAGGYIPIDRGDRSTARQSFTAAVAHLRKGTS
                     IFLFPEGTRATTDELLPFERGGFLMAMKSGLPIVPVGIRGTRAIQHKGNWAIRPGKVT
                     VSFGAPLDPAAYGIRRKNELIHEVRRQVAEMAGLRLPEGEGGPPEVESGGEA"
BASE COUNT          944 a         2098 c         1966 g          899 t
ORIGIN      
        1 ttacaatgac cataccgccg gcaagggcga cctcttcctc atggtccagg gccaggacct
       61 ccagccgcag ctgcgggagc cgaaggccat ccgcccccgc ccgctcgacc gggcgctgcg
      121 cgagaagctc gaggccacca tcgcggcgaa cgtccgggcg atccggcgaa gccctctgcg
      181 ggtcgcgtac gccctggacg atgaggtctc ctggagcgtc ctctcgcggc cgatcccctg
      241 gcgcatccac ggcgacgacg gcgactaccg caggtggctc cactctgttt acggacgcgg
      301 cgcccccggc gttcagatcg tcggtcccga cttcgccctg gagcagctcg gccgtcctct
      361 cgggaggctc gacttctccc ccttcctgga ccggatgtcc tacaacgact cggtctgggc
      421 gaacctgctg ggagccctgg tggagcgctg ccatcgggag gacccctcca ccccctgcgg
      481 cttcgtgggc ggccaggccc ccggcctctg ggggggctac gactacgcca agatcgccag
      541 gaaggtgcag ttcatcgagg cttacgacct cggctcgtcg gcggagatcc tgcgctcgtt
      601 cgccccggac atcccacggg tcaccacgca tttccacaag gatcagcggt cccccggcaa
      661 cgaccgttac ctcgcctggc gctacttcgc ccacggccac cggggcatga tcggctgggt
      721 ggacgagagc tggttcgagg ggggcagacc tcgcccctgg ctcgaccgtt tccggcccgc
      781 gctccgcgag ctcgggggcg tccagggccc aaagatggta ggcgcccggc cgctcgacga
      841 tggcatcgcc ctctactact cccatccgtc tatccaggtc tcctggatcc tcgacgcgga
      901 ggcgcacggc aagacgtggc cgaaccgcaa cgaagaccac cgtctgggca cctcccacaa
      961 cgtccgccgc gcctggacct ggctcctgga ggacgccggg ctccagtacc gcttcctcgc
     1021 ctatgaccag gtggtgaccg gcggcgtccc cgcggacgtc cgcgtgctga tcctcccagc
     1081 ctgttatgcg ctgtcggacg tcgaatcccg tcacatcgcc ggcttcgcgg cggccggagg
     1141 cacggtgatc gccgacttcg cccccggcct cttcgaccag cacggcaagg ggaggcttcg
     1201 cggcgccctc gacgacctct tcggcgtccg ccacgacggc accgagaccc gcgccgactt
     1261 cttcggcggc cgcctctggg tggagaccga ccaggacgcc ggctactcct tccggcgcta
     1321 ccgtgagctg ctggagacgg tccgcccgcg cctgcaccag ggctatgcca tcgctgagag
     1381 gaatctaccc gtggggaccg cccgccaggt gggcaagggc cgggcggtct acctgaacct
     1441 ctcgccgcag cgctatctga tggagaggga ggagaggacg gcgacggagg cgcaccgccg
     1501 cgtcttcctg gagccggtct tcgcggcggg ggtcgccccg tggatccggg cgaaggcgga
     1561 ccgggcgatt ccgctggaag tcacctactg gtcgaaacag gggaggacgc tcgtcttcgt
     1621 catccagaac gttcccgtct cgggcagctc gacgggaggc gggggcgccg aggggctggt
     1681 ggaggcggtc gtcccggtgg aggtccgcct ctccatgccg gtgcgggacg tccgcgacga
     1741 gcgcaccggc cgcaagctcc ccgacggcga ccgcttcacc ttccagctcg acacgaccga
     1801 ggcggtcttc ttcagcttcg cgggagagcc gccaaccact ccacccgccg gctcctgaac
     1861 caggcattga ggagccgcac ctccccgccc ttcacctgcc agacccgcgg ctcgtggaag
     1921 tggccgagga ggaggacgtc gtgtccctcg gcgaggcggc gctcggcgta ggcgcggatg
     1981 gcctcctcgg ggagcgcggc cttgtgcttg aagttggtct gggacagccg ctgctcggtc
     2041 gagctgacca ttcggcgggc gaggccgcgc ggcaggttga ggacagccag gcgcaccggc
     2101 gcgctcttgg agagccagcg ccagaacagg tattgccggt ccttgtcgtt cagcccgtcg
     2161 ccatggacgg cgaggtaccg caccccgtcc tcctcgaagg cgaactcgac gccgatggcg
     2221 tcgaaggcgt cggcgtaggg gctgccggtg aggaagaaat cgcggttccc ctccacgtag
     2281 tccacccgca atccctgacg gcgaagctcg cggagcaccg gcaccaccgc cgcgatctcc
     2341 gccgtctcat accggggatc gccgatccac gcctggaaga tgtcccccat gaggaccaac
     2401 cgggtacacc cctcccccgg cagcgcccgg agctggcgga tcatcggctc ggaggacccg
     2461 ccgggtcccc ccaggtgcgc gtcggcgatc agggcgatgg acatgggagt aggcctgctc
     2521 cccgtcgagg ctttggaatc aatgagatgc cctcacctgg gattgaaacc caggctttat
     2581 gcctatgccc cttcgaggcg ttgagggttg gcgcagagcg gaaatctggg cgtgcatcgg
     2641 acctcttggg ccacacggca gcgagtctat ctcaccatct ccggaagccc cggggcgagg
     2701 cgtccggccc gcgatgaagg agccttccat ctcgggtcga accgttccaa ccagggtcga
     2761 acggccgcca actcgaatgc ccctccgagg gcagcagcgg ctgcccgcgg agggacccct
     2821 cgcatcaacc cccgaaaccg cagctatcgg ggtcgacgtc acacgaattc ggcccacagc
     2881 gccactgcaa gggaccgaag gcgcgatcgc accagcatgg ataaaccgaa gtgtacccag
     2941 tgctgtcatc gtacaaagta cagaaactgc gtgcccctcc ccttgtgcag gcggctccgt
     3001 ccttggtcga gcagtccgcg gcgtaggcag gtcgtgggct cgaaaacacg cagatgacga
     3061 ggatcccagc gagcgccgcc aatcgtaaag cgttcttcat gatgtctcct ctgtgacgcc
     3121 ggggaccggc ccccggcccg cggattctat cccgccctga ctctccaaca gccggaagcc
     3181 ggcggagcgg aagccctcgg ccgttgcgga tcgatcctcg acgagcgggt gtagaaagca
     3241 agaacgccgt cgcctgctcc gccggctcgg gagcaggccg cggcgttgct gttggaggga
     3301 tgtctctcgg atcgcatccg gacgccgcgg atctcgtccg gacgctccgg gaagcttccc
     3361 gagccccccg gatcactccc gattcactcg gatggagtcc aggcgctccg gatggcatcc
     3421 gcggcgttcg gatggcatcc gcacggcccg ggaagcttcc cgggcgcttc ggatggcgtc
     3481 ccattcaatt ggatggagcc cgaagcgtcc ggatggagtc cgggccgtgc ggatcaggcc
     3541 cctacacgat cgtcagcttc ggcttggccg ccgcctggcg cgccacctcg gcgaccacgg
     3601 actcggcgac ctgccggaag gcgccggcgt gggggccgcg gggatcggcg acgacgatgg
     3661 gcacacccgc gtctcccccc tcgacgatct tggcgtccag ggggatggag ccgaggaagg
     3721 cggtctccag gagatcggcg gtgcgcttgc cgccgtcctt cttgaagatg tccgtcgcct
     3781 cgccgcagtg cgggcagatg aaggtggaca tgttctcgat gatgccgatc accggcacgt
     3841 tcaccttgcg gaacatcgcg aggcccttgc gcgcgtcgat cagcgccacg tcctgcggcg
     3901 tgctgacgat cacggcgccg gagagcggca ccttctgggt cacggtgagc tgcgcgtccc
     3961 ccgtgccggg cggcaggtcc aggatcagat agtcgagggc gccccactcg acgtccccca
     4021 gcatctgctc gatcgcccgc atcaccatgg ggccgcgcca gatgaccggc gtgtcggtct
     4081 ccaggatgaa gccgagggac atcacccgga tgccgtactt ctcgaagggg aagatgcggt
     4141 tgcccaccac ccgcggcttc tcggtgatgc cgaacatcat cggcaccgac gggccgtaga
     4201 tgtcggcgtc gaggagcccg acgcggtggc cgagcatccc cagcatgatc gccaggttgg
     4261 ccgccacggt ggacttgccg acgcccccct tgccgctcgc caccgccacc acgtgccgca
     4321 cctcggggat gagcgacgag tcctgggcaa tcgcacgctg cgccgtctcc tcccgcgagg
     4381 ccggccgctg gacgttcagg ttcacctgca ccgccgagat ctccgggatg gcggcgaccg
     4441 cgcgctcggc cgcctcccgc acctgacccg ccgcggcctc gttctgggtg gagatgacca
     4501 ggtccaccgt cgccacgccg ccggatacct tcacctggtg cacgaagccg aaggagacga
     4561 tgtcccggcg catccccggg aacgtcaccc ccttcaaggc atcccacacc ctggactcga
     4621 catcgttcgc cataagctcc tcggttctta tcggggatcg cggcgcccgg ctgaacaacc
     4681 aacggagggc gccgcggagc aagcgtacca tctcattcgc cggccagccg caggacgttc
     4741 gcgcgagtcc ctgcgtccag ccggaaattg aggcgctcga gccggtcgag aaggggtgcg
     4801 agccggtcca cccggccggt ccgcctggcc ttcagcagaa ttcccagggt accggtcatc
     4861 cggagcctga gcctgtcggc aaaccggcgg gccagaaagt cgtccagaac gaccagagag
     4921 ccggtgctct ggagcgcgag cgccagcacc tctcgctccc cggttcccag gtccgtcacc
     4981 agaggcagga ccgaggggct catgacctcc cggatctcga gccagggcag agaagagagg
     5041 gaggggagag agactccccg gtcgaggccc gccgcgatct cccgcgcaac gcctccgggc
     5101 accaccacgt cgccgtagag ctcgggcagg aggtccagca gatcgagctg aaagaggtac
     5161 tgcagaggcg aggtgttgac gatgacctca ggcattctcg aggtctcgcc gcagctcctc
     5221 ttcggtcaga cggaaggtgg ggacgccgta gtcacccagc ttggtgagga acagcgcctt
     5281 gggaatcccg gccaatcggg cggcggctcc ggacgagagc ttccccagct catagagctt
     5341 gacggcagcc gccaggcgca gctcctcccc catctcgtcg gcggacacct tcagcgccag
     5401 gagggaatcg tcagggatgt cgaggagtat ccggctcatc atcacgcctc tcctccggat
     5461 tctacttcag gaggcccccc ctccccctcc ggcaaccgca accccgccat ctccgccacc
     5521 tggcggcgga cctcgtggat cagctcgttc ttgcggcgga tgccgtaggc ggcggggtcg
     5581 agaggggcgc cgaaggagac ggtcaccttg ccgggacgga tggcccagtt ccccttgtgc
     5641 tggatggcgc gggtgccgcg gatccccacc ggcacgatcg gcaggccgct cttcatcgcc
     5701 atcaggaacc cgccgcgctc gaaggggagg agctcgtccg tcgtcgcccg cgtcccctcg
     5761 gggaagagga agatggaggt ccccttgcgg agatgggcca ccgccgcggt gaagctctgc
     5821 cgggcggtgc tccggtcgcc gcggtcgatg gggatgtagc cgcccgcctt catcgcccag
     5881 ccgaagaccg ggatctggaa gaggctc
//
LOCUS       DASPJI010000604         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_386040, whole genome shotgun sequence.
ACCESSION   DASPJI010000604 DASPJI010000000
VERSION     DASPJI010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386040"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1175)
                     /locus_tag="VEL74_16860"
     CDS             complement(54..1175)
                     /locus_tag="VEL74_16860"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYG64250.1"
                     /translation="MKERVLVVDDEPAIRGLLLACLSGLPLECRAVGSAAEALALASE
                     PPHPSLVISDVEMPGLSGIELLERLKALDETVQVVMVSGLQHMETVRHCLRAGAYDYI
                     VKPFEIDDLLNTVERALERARLIRQNEAYRRNLERMVAEQTAEIRQTRDIALLTLARL
                     AESRDHATGQHLERMAAYSRRLGEELATGRFPLRVSAEFVEMLYKSSPLHDIGKVGIP
                     DAILLKAGPLDPEERAVMRTHPQIGGDTLRSVIEELEGHTFLGMGMDIAYGHHERWDG
                     SGYPRGLAGGDIPLAARVVALADAYDAITSRRPYKLAHGHEEAVRRIAADRGRHFDPA
                     LVDAFLSCRDDFDSIRRCTTPAERAGSAARSGLYAVGGG"
     gene            1459..2205
                     /gene="gpmA"
                     /locus_tag="VEL74_16865"
     CDS             1459..2205
                     /gene="gpmA"
                     /locus_tag="VEL74_16865"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HYG64251.1"
                     /translation="MIKLVLLRHGESTWNLENRFTGWTDVDLSPKGLEEAREAGRLLR
                     EGGYSFDVAYTSLLKRAIRTLWLTLDGMDLMWLPVHKSWRLNERHYGALQGLNKAETA
                     AQYGEAQVHVWRRSYSDPPPPLTPDDPRYPGQEPRYAGLSPEELPLAESLADTVARFL
                     PYWHGTIVPDLRAGRKVLIAAHGNSLRALVKHLDQISEEEIVGLNIPTGIPLVYELDD
                     DLRPLRHYYLGDPDAARKAAEAVAKQGKAT"
     gene            complement(2219..>2711)
                     /locus_tag="VEL74_16870"
     CDS             complement(2219..>2711)
                     /locus_tag="VEL74_16870"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYG64252.1"
                     /translation="LGAERVFAIDVLRERLALARRFGAEPLALQDDPSDAISAVREAT
                     GGRGADPVLEAVGSPEATRLAYELARPGGTLSAVGVHTETRFALAPGEIYDKNLTYHA
                     GRAPARRYMERLLPLAEARAGDLAALVSHRLTLAEGPRGYDLFDRRLEGCTKVVLFPG
                     GAE"
BASE COUNT          415 a          969 c          961 g          366 t
ORIGIN      
        1 gtccggcccg cccgggtaac ccggatcggg ggggccggac gcctgatcga ggctcagcct
       61 ccgccgacgg cgtagaggcc cgagcgggcc gcggagcccg cccgctccgc cggcgtcgtg
      121 caccggcgga tcgaatcgaa atcgtcacga cagctcagga acgcgtccac gagcgccgga
      181 tcgaaatgcc ggccgcggtc cgcggcgatg cggcggaccg cctcttcgtg gccgtgcgcc
      241 agcttgtacg gccggcggct ggtgatggcg tcgtaggcgt cggccagggc gacgacgcgc
      301 gccgccagcg ggatgtcgcc gccggccagc ccgcgcgggt agccgctgcc gtcccagcgc
      361 tcgtgatggc cgtaggcgat gtccatcccc atgccgagga aggtgtgccc ctcgagctcc
      421 tcgatgacgc tacgcagcgt gtcgccgccg atctgcggat gggtccgcat gaccgcccgc
      481 tcctcgggat cgagcggacc tgccttgagc aggatggcgt cggggatgcc caccttgccg
      541 atgtcgtgca gcgggctcga cttgtagagc atctcgacga actcggccga gacccgcaac
      601 gggaagcggc cggtggccag ctcctcgccc aggcggcggc tgtaggcggc catgcgctcc
      661 aggtgctggc cggtggcgtg gtcgcgcgac tcggccaggc gggcgagggt gagcagggcg
      721 atgtcccgcg tctggcggat ctcggcggtc tgctcggcca ccatgcgctc caggttgcgc
      781 cggtacgcct cgttctggcg gatgagccgc gcccgctcga gcgcccgctc caccgtgttc
      841 aggaggtcgt cgatctcgaa gggcttgacg atgtagtcgt aggcgcccgc ccgcaggcag
      901 tggcgcacgg tctccatgtg ctggagcccc gagaccatga ccacctgcac ggtctcgtcg
      961 agcgccttga gccgctccag cagctcgatg cccgacaggc ccggcatctc cacgtcggag
     1021 atcaccagcg acgggtgggg cggctcggag gccagggcca gggcttccgc cgcgctgccc
     1081 acggcacggc actccagggg gagaccggag agacaggcga gcagcagccc gcggatggcc
     1141 ggctcgtcgt cgacgacgag gactcgttcc ttcatggcac ctcgacgcgg accggccccc
     1201 cgacggcaac gctgggctcc ggcaccgcga tccggaccac ccccaacatc gggagcgtcc
     1261 accctgcgcg agggccgttt cgcggcggcg gagagatccc ggacgcaaga gccccggatc
     1321 tcaccgctct gagacaacat tcaatacact acaatagaaa aaaactgcaa cggctggaat
     1381 tgtacacgtc tcgtgccaac ccgccaaagc ggcgccgctc tgttgatatc ctttgccggc
     1441 tttggaaagg aggggggaat gatcaagcta gtccttctgc ggcatggaga gagcacctgg
     1501 aaccttgaga accggttcac cggctggacc gacgtggacc tctcgcccaa ggggctggag
     1561 gaggcccgcg aggcggggcg cctgctccgg gaggggggct acagcttcga cgtggcctac
     1621 acctcgctgc tcaagcgggc gatccgcacg ctctggctca ccctggatgg catggacctg
     1681 atgtggctcc cggtccacaa gagctggcgc ctcaacgagc gccactacgg ggcgctccag
     1741 gggctcaaca aggccgagac cgcggcccag tacggcgagg cccaggtcca cgtctggcgc
     1801 cggagctact ccgatccgcc gccgccgctg accccggacg acccgcgcta cccggggcag
     1861 gagccgcgct acgccggcct gtcccccgag gagctgccgc tggccgagtc gctggcggac
     1921 accgtcgccc gcttcctccc ctactggcac ggcaccatcg tgccggacct gcgcgccggc
     1981 aggaaggttc tgatcgccgc gcacggcaac agcctgcggg cgctggtcaa gcacctggac
     2041 cagatctccg aggaggagat cgtcggcctc aacatcccca ccggcatccc gctggtctac
     2101 gagctcgacg acgacctccg gccgttgcgg cactactacc tgggcgatcc cgatgccgcc
     2161 cgcaaggccg ccgaggccgt ggccaagcag gggaaggcga cgtagcggac agggtgcgct
     2221 actcggctcc gcccgggaag aggaccacct tggtgcaccc ctcgagacgc cggtcgaaca
     2281 ggtcgtaacc ccgcggcccc tctgccagcg tcaaacggtg ggagacgagg gcggcgaggt
     2341 ccccggcacg ggcctcggcc agcggcagga gccgctccat gtagcggcgg gccggggcgc
     2401 ggccggcgtg gtacgtgaga ttcttgtcgt agatctcccc cggcgcgagg gcgaagcggg
     2461 tctcggtgtg gacgccgacg gcggacaggg tgccgccggg gcgggccagc tcgtaggcga
     2521 ggcgcgtggc ctccgggctc cccacggctt cgaggaccgg gtcggccccg cgcccgccgg
     2581 tcgcctcccg caccgccgat atcgcatcgc tcggatcgtc ctggagcgcc agcggctcgg
     2641 cgccgaacct tcgggccaga gcgagccgct cgcgaaggac gtcgatcgcg aagacccgct
     2701 cggcccccag c
//
LOCUS       DASPJI010000605         6247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_150138, whole genome shotgun sequence.
ACCESSION   DASPJI010000605 DASPJI010000000
VERSION     DASPJI010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150138"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /locus_tag="VEL74_16875"
     CDS             complement(<1..1704)
                     /locus_tag="VEL74_16875"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG64253.1"
                     /translation="MPAETTHDQDNLRRVAGALADPAMPVILLSGERGSGRRDLLATA
                     AARTALDVVSLDLEGYEDGDQGLLRFLALQAEKGVNQERLTAARQLAEALPPTPSAAA
                     LLALVLGSVPGSPHPVLDFSGLTLWDAFRRLLESPKRLVLHVENGAGLDAVLRRRLID
                     EARRGTGVALVLSCHPRDRSEDLAPGGEVLRVEMAAPERSGESQEVLRSLLADLDLQV
                     ADRLERFLDLAALCGANVPAELLLAHIELDEEARDEILDVIDEELVETGDNRLFLDLQ
                     YRHPSFPGLLTYAFVSPSQAHALLGSVTADKRARLASELLEYLGRHVSVATRGLALLF
                     LSLADHLDNPEPRERFRRELAWWAGPDDAGELAGVVGEELAMGRVPPAEILRLAVESR
                     QRWPPFRRFALLEALDRHRDAVPVDHQGDLRFLRAQTLGDLGRHAQALEEARMALELV
                     ESTRGRETPDTAGVLALVGILSRLSGNPQDGLGYLERALRIDRQVLGPSHPEVAAILA
                     NLGAVQRELGNPRDARASLEEALAIYRRAVGDLHPGTASAYEGLGEVLMDLGETDGAR
                     EQ"
     gene            complement(1797..4568)
                     /gene="cphA"
                     /locus_tag="VEL74_16880"
     CDS             complement(1797..4568)
                     /gene="cphA"
                     /locus_tag="VEL74_16880"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HYG64254.1"
                     /translation="MKILDTRVYRGPNVYALYKLIRLRVDLGELEDYPTNRLPGFTER
                     LLEWMPTLHEHTCSYGVPGGFVRRMTEDEGTWLGHVLEHVAIELQCLAGTPVSYGKTR
                     SHDLPTGQYHVIYSYEEETVGLRAGELALDVIRHLLPPERAAHASAPFDYQAELEDLI
                     RLAQRRAFGPSTASLVRAAEERDIPWIRLNDRSLVQLGHGKYQQRIQATVTSQTRHTA
                     VEIASDKRLTNQILHDLGLPVPRQERVRTLEGALEAAEKLGYPVVVKPLDGNHGRGVS
                     INLRTPEQVRVAFDEAYERSSVVIVETYLEGHDYRILVVNGKVVAVAQRVPGHVVGDG
                     RHTIAELVDLVNGDPRRGVGHEKVLTRLEIDHQAERLLEQAGYNLGTVLPEGEVLYLR
                     STGNLSTGGTAIDKTDVIHYDNRAMAERAVRAVGLDVGGVDYISPDITRSYREVGGGI
                     VEINAAPGFRMHVAPTEGKPRDAAGPVIDMLFPKDTPYRIPIAAITGTNGKTTTTRMV
                     GHILKLAGHTVGMATSDGVYIDGVLTVQGDMTGPWASHLVLRDPTVDMAVLETARGGI
                     VRSGLGWRRCTVGAVLNVAADHLGMGGIDDLDELAEVKRVVVEVARDFCVLNADDERV
                     ARMADHSPGKPVYVTMQRNNERVRRHIRDKGRAIALEEGLNGRMIVLYEGEDHVPLLW
                     ARQIPATIDGHALHNVQNAMFAAAIAISMGVSLENVRQGLRTFTTDFYQTPGRMNFYN
                     EHAFRVLLDYAHNAHGMTAMAQTVREMTVHGRRLGVVSAPGDRRDEDILDVARAAAPA
                     FDLILLREDDNRRGRERGEVGELLRQGLLAAGFAADRIVPGVFDEPEAVQRSLETAQP
                     GDLLVIFGDKLDRDWQQIVSFGKTGGAPAEAPVSAEVIAAAVPEQQDLLAAVRAGDSL
                     SAETGEHDD"
     gene            complement(4565..5479)
                     /locus_tag="VEL74_16885"
     CDS             complement(4565..5479)
                     /locus_tag="VEL74_16885"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HYG64255.1"
                     /translation="MPSHPSSPSAASHPGLLIAVGGAEDKVRERLILRYFLEACGGSD
                     AHVVVLATASELPETGGRYADLFYQMNADTVEVLRIANREEALDAGAEAHDLLEYASG
                     LFITGGSQLKLSSALGGTPIADAIRRRHAAGMVVAGTSAGAALLSEHMIAMGDSGGTP
                     RRRLVQMAKGLGLAPGVIVDQHFRRRDRLGRLLTALSYNPGLLGMGVDEDTAAIFDPD
                     GTVSVLGSGAVVMVDASGMRFTDSYAVQQGQPVAMLGLKVDFLTTGCRYDLKRRAGMP
                     PAVVHRASELEAAEAAVAAVMGVGSPRS"
     gene            5626..5778
                     /locus_tag="VEL74_16890"
     CDS             5626..5778
                     /locus_tag="VEL74_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64256.1"
                     /translation="MKKKTKKLVLAKETVRELKNPEGLKEAAGGARTDYPSCGVSCFY
                     FETCFC"
     gene            complement(5887..>6247)
                     /locus_tag="VEL74_16895"
     CDS             complement(5887..>6247)
                     /locus_tag="VEL74_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HYG64257.1"
                     /translation="EALVPGGWPEIRRRNRELALAARRILSEALGVKPPAPEEMIGSL
                     VALPLPDGSPEPPVSALYADPLQEDLLTRYKIEVPIVPWPEPPRRLVRISAQLYNSEE
                     EYQRLGEALRELGMVSM"
BASE COUNT          898 a         2206 c         2239 g          904 t
ORIGIN      
        1 ctgctcccgc gcgccgtcgg tctcgccgag gtccatgagg acctccccga gcccctcgta
       61 ggccgaggcc gtgcctggat ggaggtcgcc cacggcgcgg cgatagatgg ccagcgcctc
      121 ctccagggac gcccgcgcgt cccgcggatt ccccagctcg cgctgcaccg cgcccaggtt
      181 ggcgaggatc gccgccacct cgggatggct cggtcccagc acctggcggt cgatccgcag
      241 cgcccgctcc aggtagccga ggccgtcctg ggggttgccg gagaggcgtg acaggatccc
      301 aaccagggcg agcacaccgg cggtgtccgg ggtctcgcgg ccgcgggtgc tctcgaccag
      361 ctcgagggcc atccgcgcct cctccagcgc ctgggcatgc cggccgaggt cccccagggt
      421 ttgggcgcgc aggaagcgga gatccccctg gtgatcgacc gggaccgcgt cgcggtggcg
      481 gtcgagggcc tcgagcaacg cgaagcggcg gaaggggggc cagcgctgcc gggactcgac
      541 ggcgagccgc aggatctcgg cgggcggcac cctccccatc gccagctcct cccccactac
      601 acccgccagc tccccggcgt cgtccggccc cgcccaccag gcgagctccc gccggaagcg
      661 ctcccgcggc tccgggttgt ccaggtgatc ggccagggag aggaagagga gggcgaggcc
      721 gcgcgtggcc acggagacgt ggcgcccgag gtactccagc agctccgagg ccagccgcgc
      781 ccgcttgtcg gccgtcaccg agcccagcag ggcgtgggcc tgggaggggc tcacgaaggc
      841 ataggtcagc aggccgggga acgacgggtg acggtactgg aggtcgagga acagccgatt
      901 gtcgccggtc tccaccagct cctcgtcgat gacgtcgagg atctcgtccc gcgcctcctc
      961 gtccagctcg atgtgggcga ggagcagctc ggccggaacg ttggcgccgc acagggcggc
     1021 caggtcgagg aatcgctcca gccggtccgc cacctggagg tcgaggtccg ccagcaggct
     1081 ccgcaacacc tcctgggatt caccggatct ctccggcgcc gccatttcca cccgcagcac
     1141 ctcgccgccc ggcgcgagat cctcactccg gtcgcgtgga tggcaggaga ggaccagggc
     1201 cacgccggtg ccgcgccggg cttcgtcgat caaccggcgg cgcagcaccg cgtcgagccc
     1261 ggcgccgttc tcgacgtgca ggaccagccg cttcgggctc tccaggaggc gccggaaggc
     1321 gtcccacagg gtgagcccgg agaaatccag aacgggatgc ggcgatcccg gcaccgatcc
     1381 caacacaagg gccagcaggg ccgccgccga gggggtggga gggagcgcct cggcgagctg
     1441 ccgcgccgcg gtcagccgct cctggttcac ccccttctcc gcctggaggg cgaggaagcg
     1501 gaggagcccc tgatcgccgt cctcgtagcc ttcgaggtcc agggagacca cgtcgagcgc
     1561 ggtacgcgcc gcggcggtcg caaggaggtc acggcgcccg ctcccccgct ctcccgagag
     1621 gaggatcacc ggcatggccg gatcggcgag cgctccggcc acccgtctga ggttgtcctg
     1681 gtcgtgtgtg gtttccgcag gcacgagctc ctccattcct gacgagcggg gatcgtacag
     1741 gaaaagaggc cttcaccccg cgcccgccga tctgatcgct ggcgcgtccc cctggcctag
     1801 tcgtcgtgct cgccggtctc agccgagagg gagtccccag cgcgcacggc agccaggaga
     1861 tcctgctgct ccgggacggc ggccgcgatc acctcggccg aaaccggagc ctccgcggga
     1921 gctccgccgg tcttgccgaa gctcacgatc tgctgccagt cacggtcgag tttgtcgccg
     1981 aagatcacga ggagatctcc cggctgggcg gtctccagcg acctctgcac cgcctccggc
     2041 tcgtcgaaga cgccggggac gatgcggtcg gcggcgaagc cggcggcgag cagcccctgg
     2101 cggagcagct cccccacctc tccccgctcc cggccgcggc ggttgtcgtc ctcgcgcagc
     2161 aagatcaggt cgaacgccgg cgcggcggcg cgggccacgt ccaggatgtc ctcgtcccgc
     2221 cggtcgcccg gcgccgagac cacgccgagg cggcggccgt gcaccgtcat ctcgcggacg
     2281 gtctgcgcca tggcggtcat gccgtgggcg ttgtgggcgt aatccaacag cacccggaag
     2341 gcgtgctcgt tgtagaagtt catccgcccc ggggtctggt agaagtcggt ggtgaaggtg
     2401 cgcagtccct ggcgcacgtt ctccaggctc acccccatgg agatggcgat ggccgcggcg
     2461 aacatggcgt tctgcacgtt gtgcagggcg tggccgtcga tcgtcgccgg gatctgccgc
     2521 gcccagagca gagggacgtg gtcctcccct tcatagagga cgatcatgcg gccgttcagc
     2581 ccctcctcca gggcgatggc ccggcccttg tcgcggatgt gccggcgcac ccgctcgttg
     2641 ttgcgctgca tggtgacgta gaccggcttg cccgggctgt ggtcggccat acgcgccacc
     2701 cgctcgtcgt cggcgttgag aacacagaag tcccgcgcca cctccaccac cacgcgcttg
     2761 acctccgcca gctcgtcgag gtcgtcgatc ccccccatgc ccaggtggtc ggcggcgacg
     2821 ttcaacaccg cgcccaccgt gcaccggcgc cagccgaggc cggagcggac gatgccgccg
     2881 cgcgcggtct ccagcacggc catgtccacc gtcgggtcgc gcagcaccag gtggctcgcc
     2941 cacgggccgg tcatgtcccc ctggacagtg agcacgccgt cgatgtagac gccgtcgctg
     3001 gttgccatgc ccacggtatg gccggcgagc ttcagaatgt gccccaccat gcgggtggtc
     3061 gtggtcttgc cattggtgcc ggtgatcgcc gcgatcggga tgcggtacgg cgtgtccttc
     3121 gggaagagca tgtcgatgac cggcccggcc gcgtcccgcg gcttgccctc ggtcggcgcc
     3181 acgtgcatcc ggaagcccgg ggcggcgttg atctcgacga tgccgccccc cacctcacgg
     3241 tagctgcggg tgatatccgg cgagatgtag tcgacgccgc cgacgtccag ccccacggcc
     3301 cgcacggcgc gctccgccat cgcccggttg tcgtagtgga tgacgtcggt cttgtcgatg
     3361 gcggtgccgc cggtggacag gttgccggtc gagcgcaggt agagcacctc cccctcgggg
     3421 agcaccgtgc ccaggttgta gccggcctgc tccaacaggc gctcggcctg gtggtcgatc
     3481 tccaaacggg tcagcacctt ctcgtgcccg acgccgcgcc gggggtcgcc gttgacgagg
     3541 tccaccagct cggcgatggt gtgccggccg tcccccacca cgtggccggg gacccgctgg
     3601 gccaccgcca ccaccttgcc attgaccacc aggatccggt aatcgtgtcc ttccagatag
     3661 gtctcgacga tgaccaccga cgagcgctcg taggcctcgt cgaacgccac ccgcacctgc
     3721 tcgggggtgc gcaggttgat ggagacgccg cggccgtggt tgccgtccag cggcttgacc
     3781 accaccggat agccgagctt ctcggccgcc tccagcgccc cctcgagggt gcgcacccgc
     3841 tcctggcgcg gcaccggcag ccccaggtcg tggaggatct ggttggtcag ccgcttgtcg
     3901 gaggcgatct cgacggcggt gtggcgggtc tggctggtga ccgtggcctg gatgcgctgc
     3961 tggtacttgc catggccgag ctgcaccagc gaccggtcgt tgaggcggat ccaggggatg
     4021 tcccgctcct cggcggcgcg caccagcgag gccgtcgagg ggccgaaggc gcggcgctgg
     4081 gcgaggcgga tcaggtcctc cagctccgcc tggtagtcga agggggcgga ggcgtgggcg
     4141 gcgcgctccg gcggcagcag gtggcggatg acgtcgagcg ccagctcccc cgcccgcaag
     4201 cccaccgtct cctcctcgta gctgtagatg acgtggtact ggccggtagg cagatcgtgg
     4261 ctgcgcgtct tgccgtagga gaccggcgtg ccggccaggc actggagctc gatggcgacg
     4321 tgctccagca cgtgcccgag ccaggtcccc tcgtcctcgg tcatgcggcg cacgaagccg
     4381 ccggggacgc cgtacgagca ggtgtgctcg tgcagcgtgg gcatccactc gaggagccgc
     4441 tcggtgaaac ccggcagccg gttcgtcggg tagtcctcca actcgccgag gtcgacgcgc
     4501 aggcggatga gcttgtagag ggcgtagacg ttcggaccgc gatagacccg ggtgtcgaga
     4561 attttcacga gcgcgggctc cccactccca tgactgcggc cacggccgcc tcggccgcct
     4621 ccagctcgga ggcgcggtgg accaccgccg ggggcatccc ggcgcgccgc ttgagatcat
     4681 agcggcagcc ggtggtcagg aaatcgacct tgaggcccag catggcgacc ggctgcccct
     4741 gctggacggc gtagctgtcg gtgaagcgca tgcccgaggc gtccaccatg accacggcgc
     4801 cgctgccgag gacggagacg gtgccgtcgg ggtcgaagat ggcggcggtg tcctcgtcga
     4861 cgcccatgcc gaggaggccg gggttgtagg acagcgcggt cagcaggcgc ccgaggcggt
     4921 cgcggcgccg gaagtgctgg tcgacgatga cgccgggggc gaggccgagc cccttggcca
     4981 tctgcaccag gcggcggcgc ggcgtgccgc cgctgtcgcc catcgcgatc atgtgttcgg
     5041 agagcagagc ggcgccggcg ctggtgccgg ccaccaccat gccggccgcg tggcggcggc
     5101 ggatggcgtc ggcgatcggg gtgccgccga gggccgagga gagcttgagc tggctgccgc
     5161 cggtgatgaa caggccagag gcgtactcca ggagatcgtg ggcctcggcg ccggcgtcga
     5221 gcgcctcttc gcggttggcg atccggagca cctccaccgt gtcggcgttc atctgataga
     5281 agaggtcggc gtagcgaccg ccggtctcgg gcagctccga ggcggtggcg aggacgacca
     5341 catgggcgtc ggagccgccg caggcttcca ggaaataccg caggatcagg cgctcgcgga
     5401 ccttgtcctc ggctcccccg acggcgatga gcagccccgg atggctggcg gccgaggggc
     5461 tggacggatg tgacggcatg caaggacctc ccaagctcgc gcgccggtgc gcgcggccct
     5521 acggctcagt agaacttgcc gaatggcggc ggaaatccta gcacggggcg ttcgtgcccg
     5581 gcaggtgcta tcctgtctca gtaactgcac aacaggagga cggccatgaa gaagaagacg
     5641 aagaagctgg tgctggccaa ggagacggtc cgagagctga agaaccccga ggggctgaag
     5701 gaggccgccg gcggcgctcg taccgactat cccagctgcg gggtgagctg cttctacttc
     5761 gaaacctgtt tctgctagcc acaagccgcc tctccggcac gacggcgttc ggccggagag
     5821 acacccctcc tcgcggccct cctcccgggc cctgcctgga gagggactct gcggcctcgc
     5881 cctcccctac attgaaacca tccccagctc ccggagcgcc tcccccaggc gctggtactc
     5941 ctcttcagag ttgtagagct gcgccgagat ccgtaccagg cgccggggcg gctcgggcca
     6001 ggggacgatc ggcacctcga tcttgtagcg ggtcagcaga tcctcttgca agggatctgc
     6061 atagagagct gaaacgggcg gctccggcga gccgtcgggc agcggcaggg cgacgagcga
     6121 gccgatcatc tcctcgggcg ccgggggctt cacccccagc gcctcggaca ggatgcggcg
     6181 cgccgccagg gccagctccc ggttgcggcg gcggatctcg ggccagccgc ccggcaccag
     6241 cgcctcc
//
LOCUS       DASPJI010000606         3906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_208909, whole genome shotgun sequence.
ACCESSION   DASPJI010000606 DASPJI010000000
VERSION     DASPJI010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208909"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /locus_tag="VEL74_16900"
     CDS             complement(<1..1543)
                     /locus_tag="VEL74_16900"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYG64258.1"
                     /translation="MSNRMIRMLTLFVLTAPALASPAAAASFIVRCDGGCATATAAIQ
                     QIPGARIDRAFANVDALVVDVPVAAVASLKSRPGIAEIQEDVAVLSTDPSDEQPVGPA
                     AGAQALSPAQIASLPAGSDYNAPLVGAHGFHAQGRFGAGVVVAVIDSGVANDPAVVPA
                     LAGSVLGGENFLPASLDPVLSATSTANDPHGTFAAVLLAGHALYRFPTASPFIQSLLA
                     HAPQSVLSCASLGCPAGESAVAVAGVAPAAKIYAMKILPSNGTAGPSSRVLMAMDRAI
                     TLRRNFDAGLPAVPVNPGCGAAENPCVYDSLPIRIANMSFGGPALYAGGSVRDLLVTE
                     MLEAGILPVTATGNDGPAALTVQSPGSGPGALNVTAATSAAHERVRRDLELGLGAGLQ
                     LRPAGNLQIQGTSSRGPLPDGRLGIDLAADGYALLSQGADGRLSLVSGTSFAAPLAAG
                     AAALLGERFPAAPVVEIRNALVEGADPSLLTDGSGPVDHGAGFLQIAAADALLAAGGV
                     SPHLPH"
     gene            2003..2359
                     /locus_tag="VEL74_16905"
     CDS             2003..2359
                     /locus_tag="VEL74_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64259.1"
                     /translation="MPPCTDNFGTFPAPATIRVLTRPEDQNVVYSLSVVVQARDSEVV
                     FEHFVHSRTDLENGAATWTLEPSRVYTLALITLGALTLRTVIEVDGNPIVNSSCSNSG
                     AGVVGVWTIPTGGGAS"
     gene            2371..3825
                     /locus_tag="VEL74_16910"
     CDS             2371..3825
                     /locus_tag="VEL74_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64260.1"
                     /translation="MIWMVVLLTPVLAWASPQDRPEQREDETLRDYLNRVAPIEQVTP
                     EDQADVATDLVSSAVTEQAVSPMGTATASSFGDRVSNTLQNFLPLFQGAINGLDTSDD
                     ERTVTLRLNPIRGARFGELGLSASATEPSASEALLEELPSDIRPGQKEAIQKTLEDFT
                     DLTYTATYNYARRLEKTRLGEDRRWYFGRNPRLYEEVFARFVGDTLTSFAPRSNAARR
                     PFEDRLGPVAIQSLTRRELETRLGISDSERLVEILIDERRTLAALNVAAGERAELFAD
                     LVANQPQASLSAAYHDRDPLVGRNETSANLSIEMPAAPNINELLRSWRATGDWQKALD
                     DLAKRTKEIEAGTKWSIVASWKEAEPYRVSYLPTTEGAADVTLDRPRSNEISIKAQYT
                     RNATWQRLMVEGLETFPALHLAAELIDVSDDPDREDRRIVGTLTYDVPVRKGVSFPIS
                     LVYANKPEFLGDADHQFSAHFGLSLNSLFGDQAN"
BASE COUNT          681 a         1269 c         1401 g          555 t
ORIGIN      
        1 cgtggggcag gtgcgggctc acccccccgg cggcgaggag ggcgtcggcc gccgcgatct
       61 ggaggaagcc ggcgccgtgg tccacggggc cggagccgtc ggtgaggagg gacgggtcgg
      121 ccccctcgac gagggcgttg cggatctcca ccaccggcgc ggcggggaac ctctcgccga
      181 ggagcgccgc ggctccggcg gccagcgggg cggcgaagga ggtgccggag accagggaga
      241 ggcggccgtc cgcgccctgg gagagcagcg cgtagccgtc cgccgcgagg tcgatcccca
      301 gccggccgtc gggaagcggg ccgcgcgagc tggtgccctg gatctggagg ttacctgccg
      361 ggcggagctg gaggccggcg ccgaggccga gctcgaggtc gcggcggacc cgctcgtggg
      421 cggccgaggt cgccgcggtc acgttcagcg cgccggggcc gctccccggg ctctgcacgg
      481 tgagggcggc cggaccgtca ttgccggtgg cggtcacggg gaggatcccc gcctccagca
      541 tctcggtgac cagcaggtcg cggacgctgc cgccggcgta gagggcgggg ccgccgaagc
      601 tcatgttggc gatgcggatc ggcagcgaat cgtagacgca ggggttctcg gccgcgccgc
      661 agcccgggtt gaccggcacc gccggcaggc cggcgtcgaa gttgcggcgc agggtgatgg
      721 cgcggtccat ggccatgagc acccgcgacg acggcccggc ggtgccgttc gaggggagga
      781 tcttcatcgc gtagatcttg gccgccggcg ccacgccggc caccgcgacc gccgactcgc
      841 ccgccgggca gcccagggag gcgcaggaga gcacgctctg cggcgcgtgc gcgaggagcg
      901 attgaatgaa gggggaggcc gtggggaagc ggtagagggc atggccggcc aggaggaccg
      961 cggcgaaggt gccgtgcggg tcgttggccg tgctggtcgc cgagagcacc ggatcgaggc
     1021 tcgccggcag gaagttctca cctcccagga cgcttcccgc gagcgccggc accaccgccg
     1081 ggtcgttggc gacgccgctg tcgatgaccg cgacgacgac gcccgcgccg aagcggccct
     1141 gggcgtggaa gccgtgagcg ccgacgagcg gcgcgttgta gtcggagccg gccggcaggg
     1201 aggcgatctg cgccggagag agcgcctggg cgccggccgc gggaccgacc ggctgctcgt
     1261 ccgacggatc ggtggagagg accgccacgt cctcctggat ctcggcgatg ccgggacggg
     1321 acttcaagga ggccacggcg gcgacgggga cgtcgaccac cagggcgtcc acgttggcga
     1381 aggcgcggtc gatgcgcgcg ccggggatct gctggatggc cgcggtggcg gtggcgcagc
     1441 cgccgtcaca gcggacgatg aagctcgcgg cggcggccgg gctggccagc gccggagccg
     1501 tcaggacgaa gagcgtgagc atccggatca ttcgattcga catctttcct tcctcccctc
     1561 gaagagagag aaacagtgga gcgggcctcc gcacggagac ccggcgagac cggccggtga
     1621 aaccggtcgg ccgagtcata acggcctgtt cgtaagaggt ggaaagctta gcagggggag
     1681 gcggagcgcg ccagagggcc gcctcccctg agaggactta gagggcgttg ccgtcccaga
     1741 tcatcaccac cgcgctgccg cccgggcccc gtcagaacga tgggggcacc ggtcctgtca
     1801 ccgctcatct cgctctgact gtaacactgt aagccagccc tctgacctcg agatccatgg
     1861 gaattcggcc cttgcggccg tctcaagatg tacatcccgg cgtgggcgag gcagctcacg
     1921 gggaaaggag ctcacgcagg taggcggttc aatctttggc gagccgaccg cagggcgggc
     1981 cggttcggtg aggaggacga gtatgccccc ttgtaccgac aatttcggca cctttccggc
     2041 tcccgcgacc atcagagtcc tgacgaggcc ggaggatcag aatgtggtct actctctctc
     2101 cgtcgtcgtg caggcgcgcg acagcgaagt ggtgttcgag cacttcgtcc atagccggac
     2161 cgacctcgaa aacggtgcgg ccacctggac gctcgagcct tcgcgggtct acacgttggc
     2221 gctcattacc ctcggcgctc tcacgctccg gacggtgatc gaagtcgacg gcaatccgat
     2281 cgtgaacagc tcgtgcagca actccggagc cggtgtcgtc ggagtctgga cgatcccgac
     2341 cggggggggg gcgtcatgaa gcgtgccgtc atgatctgga tggtcgttct cctgaccccg
     2401 gtcctggcct gggcgagccc gcaagatcgg cccgagcagc gagaggatga gacgctgagg
     2461 gactacctga accgcgtggc gccgatagag caagtcaccc cagaggacca agcagacgtc
     2521 gccacagatc tcgtgagctc cgcggttaca gagcaggccg tgagccctat ggggaccgcc
     2581 accgcctcga gcttcggcga cagggtgagc aacacgctgc agaacttcct gccacttttc
     2641 cagggggcga tcaacgggct cgatacgagc gacgacgaga gaacggtgac tttgcgcctc
     2701 aaccccatcc gaggagccag gttcggggaa ctcggcctgt ctgcttccgc aaccgagccc
     2761 tcggcatccg aggcactgct cgaggagttg ccaagcgaca tccgccccgg acagaaagag
     2821 gccattcaaa agacgctgga ggacttcacc gatctgacct acaccgcgac ttacaactat
     2881 gctcggagac tcgagaagac gcgcctgggg gaggaccgga ggtggtactt cggccgcaac
     2941 ccacggctct acgaggaagt gttcgcgcgt ttcgtcggcg acaccctcac cagtttcgca
     3001 ccccggagca atgcggcgcg cagacccttt gaggacagat tgggtccagt cgctatccag
     3061 agtctgacgc gtcgagagtt ggaaacgcgt ctggggatca gtgactccga gcgtcttgtc
     3121 gagatcctga tcgacgagag acgaaccctg gccgcgctca acgttgcggc aggggagcgg
     3181 gctgagctgt tcgcggatct ggtcgccaac cagccgcagg ccagcctgtc agccgcctac
     3241 cacgaccgtg acccgctcgt cgggcgcaac gagacgagcg cgaatctatc catcgagatg
     3301 ccggcagcac ccaacatcaa cgagctcttg cggagctggc gcgcgacggg agactggcag
     3361 aaggctctcg atgaccttgc gaaacgcacg aaggagatcg aagccggcac gaagtggtcg
     3421 atcgtggcca gttggaaaga ggcggagccc tatcgcgtga gctacctgcc gaccactgag
     3481 ggagcggcgg acgtcaccct tgaccggccc cgctcgaacg aaatctccat caaggcccag
     3541 tacacccgga atgccacctg gcagaggctg atggtggaag ggctcgagac cttccccgcg
     3601 ctccacctcg cggccgagct catcgacgtc tcggatgacc cggatcgaga ggaccggcgg
     3661 atcgtcggga cgctcaccta cgatgtcccg gtgcgcaagg gggtgtcgtt cccgatctct
     3721 ctggtgtatg ccaacaaacc cgagttcctg ggcgacgccg accaccagtt cagcgcccac
     3781 ttcggcctga gcctcaactc actcttcggc gatcaggcga actgaggttc ccgaggcgag
     3841 cctccgtcga aggacggagg ctcgccaagc ctctactgcg agatcccgtc ccagatcatc
     3901 accacc
//
LOCUS       DASPJI010000607        11241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_208944, whole genome shotgun sequence.
ACCESSION   DASPJI010000607 DASPJI010000000
VERSION     DASPJI010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11241)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11241
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208944"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VEL74_16915"
     CDS             complement(<1..1161)
                     /locus_tag="VEL74_16915"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HYG64261.1"
                     /translation="MSVAVTKTKGRDRPTISPEKGSELAGLVLFFLGLFLALSLVSYY
                     PEDPSLFHHTAESSGARNWTGAAGAHVAAVAFGFLGVTCLLVPFFLLVAAWRRLRRRG
                     AVKVVGRGFGVALLLAATPPLLQLTLGDFGWRGGAVASGGGFGTVLVELLRERLNFMG
                     TLLVLATTVICGLALAVQSTLGAVLGVWRARLQQAWQDYTLARERRKERRAKERARRR
                     VITKHLQRVMEEKQKKVEPPPPPVKQAPILSPMPSPRPAPISAPALAAVTAATGPAAD
                     SEGGRLDLPLRPLRVTERRGEAEYSVRRVSASELQEDEEFQAVREALAAPPPVSVPQS
                     ATPRVGPPPARRAAGTLPQPALPFVAEVAPGTLPPINLLQMGDGKNAVDEAEL"
     gene            complement(1560..2702)
                     /locus_tag="VEL74_16920"
     CDS             complement(1560..2702)
                     /locus_tag="VEL74_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HYG64262.1"
                     /translation="MKGSFRLVRLAGVDIKLHFTFPLILVLGAVQWASPHGLAGAVFG
                     ALLMLALFLCVALHELGHAVTAQRLGIPVREIVLLPIGGVALLSRNPRRPAYELLIAA
                     AGPLVNVILAVALFFAIGLRGGLSLAGDALLQPGAAPSLETALSWLLVANVSLVLFNL
                     IPAFPMDGGRMLRAVLAMGMGFTRATRVATVVGQILAMVLGIYAILAGQMMLALIAVF
                     VFLGAGGERAAEEARGLLSTLRLGDAYNKHALLLQPADRVSRVIDYLLTSYQPDFAVI
                     HGGRLLGVVTRDAVFQSLVSGEGDGYVTGIMQRDLLQLSSAMTLADAQERMGAEGRRV
                     AAVVDGERYLGLVNADDIREALQIAVYLRMAEERQGRAPLPHEPLV"
     gene            complement(2725..3708)
                     /locus_tag="VEL74_16925"
     CDS             complement(2725..3708)
                     /locus_tag="VEL74_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HYG64263.1"
                     /translation="MPETSLWLWVGFNLFVLGMLALDLGVFHRKAHEVSVKEATIWSC
                     VWVTLALVFNAGLWYFRGPDPALQFLTGYLIEKSLSVDNIFVIALLFSYFAVPAAYQH
                     RVLFWGILGALVMRAAFILAGTALITQFHWIIYVFGAFLIFTGIKMALHRDIEIHPEK
                     NPVLGLVRRLIPVTGDYRGKHFFVREAGRWAATPLFLVLVLVESTDLVFAVDSIPAIF
                     AVTQDPFLVYTSNVFAILGLRSLYFLLAGVMQKFSYLKLGLSAVLVFVGIKMVLADVY
                     KVPTPLSLGVIAALLTAAVAASLIKARREEAAAGRSTEGAPKEDPPVRRVS"
     gene            complement(3971..5188)
                     /locus_tag="VEL74_16930"
     CDS             complement(3971..5188)
                     /locus_tag="VEL74_16930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG64264.1"
                     /translation="MKRPSSSSLLLLPLLLLPLLLRQDAGAQAPAAPAVGGLRVSIVT
                     PRPGEPAFGDVEVKAAVQGNVTKVEFYLDGLRVGLAEAPPWEVVIDVGQQNIEHRIEV
                     VAYDRSGRTATTSLKTGVITSDEEINVALRPLFVNVARGDQPVLDLGRGDFEVFDNGA
                     RQQLVTFERGDIPFTAALLLDSSSSMEGGLLETALDGAKSFARAMKSLDEAKLLLFSD
                     RILLETPFTSAPSILTLGLGSVEAGGGTALNDSMYLALKKLEPRTGRRVLIMLSDGVD
                     VESVIPMKRVRTLAQGNQVVIYWLRLRSEEEQAEGPVPSFTAWRDVESNMREKALLRE
                     TVLESGGRILNVDSVDQVGGALARVLKELREQYVLGYYPSSSKGSGSWHDVKVQVRRG
                     GLSVRTHRGYIEP"
     gene            complement(5185..7410)
                     /locus_tag="VEL74_16935"
     CDS             complement(5185..7410)
                     /locus_tag="VEL74_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64265.1"
                     /translation="MLMESLRRTAVLLFLPSLLAAQQQGAPPPANPPASPPAAEETGA
                     ATGQLVLEAAPPAAAAPALQEPAPPPAPTGPPIRVLRTGGLKMETAALLLSGQQGGPL
                     PFALLAFPVEKQGERVRVPVLVEVDGSALLDEQQTSPLHLEVSLYAIGGAGNVQASLM
                     ETVEVDLGRMEQEVQRGGIKFVGELDLLPGDYSLRALVRNPATENIGLRVLSFTVPPF
                     EAGVRTVMAPMFSERSGAWVPVRALASRNRPAPLKALAGDDLPAARPILVLDRDMSFQ
                     VAAHQLADGTDLRLELRRPSGQLAADFPLQILGREPAGVAGIEILTATFKPHGIEPGE
                     YDLRVVIPGAGSDFRSYATSAVVAEEEVGGRVWAEFSPGGGGGRDAAGEAAKQQAQRQ
                     AAAKPRKNVDTAPIEAAYRDALRLLATGDEKGARAAVSALETRLLIDEQTLTAVDLGR
                     AQYAVARTLVQSQPESLIPVLLLHDELYRDYRNRNNSTLSIHARELVFALANLYTKQN
                     NTPAARELAARFILGLAAEFADHAPRVLRSRTFRRALDYDPANETALLCLAIDAERNT
                     EYEAAKDFLGKLLKKNPKHIEAKIRLALNESRTNRRKEARRLFEEVVRDESSGAAPVW
                     VLAVAYQELSRLLLELGAHDDAERVLRAGLRRLPEDEKLSLQLSMLLDLRKDRRESRA
                     VLEAVRPKSTPRGFDSARRRYNEIPTELLEKERTGLQQSAIERLPSLAAAAAGAKGGQ
                     A"
     gene            complement(7477..9291)
                     /locus_tag="VEL74_16940"
     CDS             complement(7477..9291)
                     /locus_tag="VEL74_16940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG64266.1"
                     /translation="MIQRFSALLSVSLLLLPLPVPAQSPAAAAPAQNDETFYDTVEVN
                     VVNVEVFVTDRSGQRVNGLTQSDFEVLEDGKPVEITNFFASEGGRAATTVLGTAAPAT
                     PEAAEIPEEQRLLLAIFVDNSNLSARARNSALPKVNEFLKTRLRTEDRVILSSYDGPG
                     SLEVRAMPTGDPATVVAALDRIARGTSGRMSGGAELRMLISQMDRSANPAGVDKEREM
                     AVMDAKDILAQIELYAEEERAKTAAMVSALEQFVDGLSGLPGRKAVLLLSGGVPLRPA
                     QALYEAWERRFGTLNQQDRSIGGSRLNAADVDLGKEMDKIGARANANRITFYALGVTD
                     SVNVTSAAASTNDLWTRTEDSLQTNNMAQSLYAIVDPTGGAATINALDPGVLLAEMRE
                     DMDSFYSLGYTPLHRRSGKSHKIEVKVKRPGLKLRHRESYRERTSAEVMSDKVLAALM
                     FGSRENPLEIGLQLGEEVSEKGKNTVQLTVKLPMSKLVLLPQGQFHEGKVTIFMAVRD
                     TQGRSSPVTEVKLPIRVPNDQLLTSLGQLAGYRAKLALRPVEHTVAVGVRDELGNIVS
                     TVTASYTPGLTPEASPAAPAATPEPAGPTEGRGDDHDR"
     gene            complement(9340..11085)
                     /locus_tag="VEL74_16945"
     CDS             complement(9340..11085)
                     /locus_tag="VEL74_16945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG64267.1"
                     /translation="MIQRFLILSLVTLLALSGPVPAQQPAQNDETFHDTFEVNVVNVE
                     VFVTDRSGQRVKGLTQGDFEVLEDGRPVEITNFYASEGAAAAVAPGASAPAVTPTAPE
                     IPDEQRLLLAVFVDNSNLSARARNSVLPKVNDFLKTRLRPEDRVILSSYDGPGSLEVR
                     AMPTGDPATVVTALDKIARGTGGRMSGGAGLRMLISQIERSGGGEEMEAADAKDILTQ
                     IRFYAEEERYKTAAMVSALEQFVDGLSGLPGRKAVLLLSGGVPLRPAQSLYEAWERRF
                     GDLNRQGKKIGGSRFDGVDVDLGMEINKVGARANANRITFYALGVTDTVNVGSAAVTS
                     SDAWTRTEDTLETNNMAQSLFAIVAPTGGAATINALDPGTLLAEMREDMDSFYSLGYS
                     PSQRRSGKSHKIEVKVKRPGLKVRHRESYRERTSSEVMSDKVLAALMFGSRENPLEIG
                     LQLGGPGTEKGKGAVPVTVKLPLSKLVLLPRGEFHEGRVTIFVAARDSEGRNSPITEV
                     KVPIRVPSRQLLAARGQLAGYRTKLTLRPLEHTVAVGVRDELGNIVSTVTAAYSPAAG
                     PAAEPAAPEGRGAKK"
BASE COUNT         1661 a         3960 c         3957 g         1663 t
ORIGIN      
        1 cagctccgcc tcgtcgaccg cgttcttgcc gtcccccatc tgcagcaggt tgatcggcgg
       61 cagcgttccc ggcgcgacct cggcgacgaa gggcagggcc ggctgcggca gcgtgccggc
      121 ggcccgccgg gccggcggcg gcccgacgcg cggcgttgcg ctctggggaa cggagaccgg
      181 cggcggagcg gcgagggcct cgcgcaccgc ctggaactcc tcgtcctcct ggagctccga
      241 ggcgctcacc cggcggacgg agtactcggc ctcgccccgc cgctcggtga cccgcagcgg
      301 ccgcagcggc aggtcgagcc ttcccccttc gctgtcggcg gcggggccgg tggccgccgt
      361 caccgcggcg agcgccggcg ccgagatcgg ggctggcctc ggggagggca tgggcgacag
      421 gataggcgcc tgcttgaccg gcggcggcgg gggctcgacc ttcttctgtt tctcctccat
      481 cacccgctgc aggtgcttgg tgatgacccg gcgacgcgcc cgctccttgg cccggcgctc
      541 cttgcggcgc tcgcgcgcca gggtgtagtc ctgccaagcc tgttgcaggc gcgccctcca
      601 gacgccgagc acggcgccca gcgtcgactg gaccgccagg gcgaggccgc agatcaccgt
      661 ggtagccagc accagcaggg tccccatgaa gttgaggcgc tcgcgcagca gctcgacgag
      721 gacggtgccg aagccgccgc ccgaggccac ggcgccgccg cgccagccga agtcgccgag
      781 ggtgagctgc aggagcggcg gcgtcgccgc cagcagcagc gcgacgccga agccgcgccc
      841 caccaccttg accgcgccgc gccgccgcag ccgccgccag gcggccacca gcaggaagaa
      901 ggggacgagc aggcaggtca cgccgaggaa gccgaaggcc accgccgcca cgtgggcgcc
      961 ggccgccccg gtccagttgc gggcgcccga gctctccgcg gtgtggtgga agaggctggg
     1021 gtcctccggg tagtagctca ccagactcag ggcgaggaag agcccgagga agaagaggac
     1081 caggccggcg agctcgctcc ccttttccgg ggagatcgtc ggccgatcgc ggcccttggt
     1141 cttggtgacg gctacggaca cgaaaccccc tgtcagctca accgagcctc tcgcccacgg
     1201 ccagtctgga agtgggggcg gtcaccctgg ctccttgaga agatggatga agaattccca
     1261 cccaagtaag catcaagtct agcacaaacc tctcccccgc cctcccgggc tccccccggc
     1321 cgccgtttcc ggagaaatcc ccgtcattcc cgcggaaagg gccgccgttt cccacgaaac
     1381 gggctccgtt tcccggggaa tggtggccat tcctgcagaa agcatggcca ttcccctgga
     1441 aggatcttcg tttccagggg aatggcgggc gtttcttcag gaatgaaggc cgtttcggag
     1501 ggaatggaga ccgtttcttc aggaatggag gccgtttccc gcgaaacggc cttcattcct
     1561 cagaccaggg gctcgtgcgg caggggcgcg cgcccctgcc gctcctcggc catgcgcaga
     1621 tagaccgcga tctgcaacgc ctcccggatg tcgtcggcgt tgaccaggcc gaggtagcgc
     1681 tcgccgtcga ccaccgcggc gacccgtcgc ccctccgcgc ccatccgctc ctgggcatcc
     1741 gccagcgtca tggcggacga gagctggagc aggtcccgct gcatgatgcc ggtgacgtag
     1801 ccgtccccct cgcccgagac cagcgactgg aagacggcgt cccgcgtcac cacgccaagc
     1861 agccggccgc cgtggatcac cgcgaagtcg ggctggtagc tggtcagcag gtagtcgatg
     1921 acccggctca cccggtccgc cggctggagg agcagggcgt gcttgttgta ggcgtccccc
     1981 agccgcagcg tcgagagcag gccccgcgcc tcctccgccg cccgctcccc tcccgcgccg
     2041 aggaagacga agaccgcgat cagcgccagc atcatctgcc cggccaggat cgcgtagatc
     2101 cccaacacca tcgccaggat ctgccccacc accgtggcca cccgggtggc gcgggtgaag
     2161 cccatgccca tcgcgagcac cgcccgcagc atccggccgc cgtccatggg gaaggccggg
     2221 atcaggttga agagcaccag gctgacgttc gccaccagca gccacgacag ggcggtctcc
     2281 agcgacggcg ccgcccccgg ctgcagcagg gcgtcccccg cgagcgacag gccgccccgg
     2341 aggccgatgg cgaagaacag ggccaccgcc aggatcacgt tgaccagcgg cccggcggcg
     2401 gcgatcagca gctcgtacgc cggccgccgc gggttgcggg agaggagcgc gacgccgccg
     2461 atgggcagca gcacgatctc ccgcaccggg atgcccagcc gctgcgcggt caccgcgtgc
     2521 cccagctcgt gcagcgccac gcagaggaag agggcgagca tcagcagcgc tccgaagacc
     2581 gccccggcga ggccgtgggg ggaggcccac tggaccgccc ccaggaccag gatcagggga
     2641 aaggtgaagt gcagcttgat gtcgacgccc gccagacgga cgagacggaa cgatcccttc
     2701 atggccggtc tccttcctgc cgcctcagga gacccgccgg acgggcgggt cctccttggg
     2761 ggcgccttcg gtggaccgcc cggcggcggc ctcctcccgc cgcgccttga tcagcgaggc
     2821 ggcgacggcg gcggtgagca gcgccgcgat cacccccagc gagagggggg tcgggacctt
     2881 gtagacgtcc gcgaggacca tcttgatgcc gacgaagacc agcacggcgg acaggccgag
     2941 cttcaagtag ctgaacttct gcatcacccc tgccagcagg aagtagagcg agcgcaggcc
     3001 gagaatggcg aagacgttcg aggtgtagac gaggaacggg tcctgggtca ccgcgaagat
     3061 ggccgggatc gagtccacgg cgaacaccag gtcggtcgac tccaccagca ccagcaccag
     3121 gaagaggggg gtcgccgccc agcgccccgc ctcgcgcacg aagaagtgct tcccccggta
     3181 gtccccggtc accgggatca gccgccgcac caggcccagc accgggttct tctccggatg
     3241 gatctcgatg tcccggtgca gggccatctt gatgccggtg aagatcagga aggcgccgaa
     3301 gacgtagatg atccagtgga actgggtgat cagcgccgtg cccgcgagga tgaaggcggc
     3361 gcgcatcacc agcgctccga ggatgcccca gaacagcacc cggtgctggt aggccgccgg
     3421 caccgcgaag tagctgaaga gcagggcgat gacgaagatg ttgtcgaccg acagcgactt
     3481 ctcgatcagg tagccggtga ggaactggag ggccggatcg ggcccccgga agtaccagag
     3541 gccggcgttg aacaccaggg ctagcgtcac ccagacgcag ctccagatcg tggcctcctt
     3601 gaccgagact tcgtgggctt tgcggtggaa gacgccgagg tcgagggcga gcatccccag
     3661 gacgaagagg ttgaagccca cccacagcca cagagaggtt tccggcattg gcctatctcc
     3721 ttggataggc cgtgggacgc gaaaaggcga gaccccacgg cacgttcgtg tcgtggtggt
     3781 ctcgccaaaa gcgcttggag ctcgcttccg gaacggccgg gggccgaagc cccgtcttga
     3841 cggccgcacc gacccaggga acacccccgg gcgggctact ccccaccgga ggagcgcatt
     3901 gtctggaact ccgggcgggt agtcaagaat tccacccgcc cggagcgtga cagttgtttg
     3961 acaaagagcc tcagggctcg atgtagcccc ggtgggtgcg gacgctcagg ccgccgcggc
     4021 gcacctggac cttgacgtcg tgccaggagc cgctcccctt ggagctcgag gggtagtagc
     4081 ccagcacgta ctgctcgcgc agctccttga ggaccctggc gagggcgcct ccgacctgat
     4141 cgacgctgtc gacgttcagg atccggccgc cgctctccag cacggtctcg cgcagcagcg
     4201 ccttctcgcg catgttcgac tccacgtccc gccaggcggt gaacgagggg accgggccct
     4261 ccgcctgctc ctcctcgctg cgcagccgca gccagtagat gaccacctgg ttcccctggg
     4321 ccagggtgcg gacccgcttc atcgggatga cgctctcgac gtcgacgccg tccgaaagca
     4381 tgatcagcac ccggcgcccg gtgcgcggct cgagcttttt gagcgccagg tacatggagt
     4441 cgttgagggc ggtgccgccg ccggcctcca cgctgcccag ccccagggtg aggatggacg
     4501 gcgcgctggt gaacggcgtc tccagcagga tgcggtcgga gaacagcagg agcttggcct
     4561 cgtccaggct cttcatcgcg cgggcgaagg acttggcgcc gtccagcgcc gtctccagca
     4621 atcccccctc catgctgctg ctggagtcca ggagcagcgc cgcggtgaag gggatgtcgc
     4681 cgcgctcgaa ggtgacgagc tgctggcgcg cgccgttgtc gaatacctcg aagtcgccgc
     4741 ggccgaggtc gaggaccggc tggtcgccgc gcgccacgtt gacgaacagc gggcgcagcg
     4801 cgacgttgat ctcctcgtcg ctggtgatca ccccggtctt gaggctggtc gtggccgtgc
     4861 gcccggaccg gtcgtaggcc accacctcga tgcggtgctc gatgttctgc tggccgacgt
     4921 cgatgaccac ctcccagggg ggagcctcgg cgagcccgac gcgcaggccg tcgaggtaga
     4981 actccacctt ggtgacgttc ccctggacgg cggccttgac ctcgacgtcg ccgaaggcgg
     5041 gctcccccgg ccgcggcgtg acgatggaca cccgcaggcc gcccaccgcc ggcgcggccg
     5101 gcgcttgagc ccccgcgtcc tggcgcagca gcagggggag cagcagcagc gggagcagga
     5161 ggagcgagga agaggatggc cgcttcatgc ctggcctccc ttcgcgccgg cggccgcggc
     5221 ggccagcgac ggcaggcgct cgatggcgct ctgctgcagg ccggtgcgct ccttctccag
     5281 gagctccgtc gggatctcgt tgtagcgccg gcgcgccgaa tcgaagccgc gcggcgtgct
     5341 cttcggccgg accgcctcca gcaccgcccg cgactcccgg cggtccttgc gcaggtcgag
     5401 gagcatggag agctgcagcg acagcttctc gtcctcgggc aggcggcgga gccccgcccg
     5461 cagcacccgc tcggcgtcgt cgtgggcccc gagctcgagg agcagccgcg acagctcctg
     5521 ataggcgacc gccagcaccc agaccggcgc ggcgccggag gactcgtcgc gcaccacctc
     5581 ctcgaacagc cggcgggcct ccttgcggcg gttggtccgc gactcgttga gcgccaggcg
     5641 gatcttggcc tcgatgtgct tggggttctt cttcagcagc ttgccgagga agtccttggc
     5701 cgcctcgtac tcggtattgc gttcggcgtc gatcgccagg cagagcaggg cggtctcgtt
     5761 ggccgggtcg tagtccaggg cccgccggaa ggtccgcgag cgcagcaccc gcggcgcgtg
     5821 gtcggcgaac tcggcggcga gcccgaggat gaagcgggcc gccagctccc gcgccgccgg
     5881 cgtgttgttc tgcttggtgt acaggttggc cagggcgaag accagctccc gggcgtggat
     5941 gctgagcgtc gagttgttgc ggttccggta gtcgcggtag agctcatcgt gcaggagcag
     6001 cacggggatc agcgactcgg gctgggactg gaccagcgtg cgggccaccg cgtactgggc
     6061 gcggccgaga tccaccgcgg tcagcgtctg ctcgtcgatc agcagccggg tctcgagcgc
     6121 cgagaccgcg gcccgtgccc ccttctcgtc gccggtggcg aggaggcgga gggcgtcgcg
     6181 gtaggcggcc tcgatggggg ccgtgtcgac gttcttcctc ggcttcgcgg cggcctgccg
     6241 ctgcgcctgc tgcttggcgg cctcgcccgc cgcgtcccgg ccgccgcccc ccccgggcga
     6301 gaactcggcc cacacccggc cgccgacctc ctcctccgcc acgacggcgc tggtggcgta
     6361 ggaccggaag tcgctgccgg ccccggggat caccacccgc aggtcgtact caccgggctc
     6421 gatgccgtgg ggcttgaagg tggcggtcag gatctcgatg ccggccaccc ccgccggctc
     6481 ccgcccgagg atctgcaacg ggaagtcggc ggcgagctgg ccgctcgggc gccgcagctc
     6541 cagccgcagg tcggtgccgt ccgcgagttg gtgggcggcc acctggaacg acatgtcgcg
     6601 gtcgaggacc aggatcggcc gcgccgccgg caggtcgtcc cccgccagcg ccttgagcgg
     6661 ggcgggccgg ttgcgcgacg ccagggcccg gacgggcacc caggcgccgc tgcgctcgga
     6721 gaacatcggc gccatcacgg tccgcacgcc ggcctcgaac ggcggcacgg tgaacgagag
     6781 cacccgcagg ccgatgttct ccgtcgccgg gttgcgcacc agggcgcgta gcgagtagtc
     6841 gccgggcagc aggtcgagct cgccgacgaa cttgatgccg ccgcgctgca cctcctgctc
     6901 catgcggccg aggtcgacct ccaccgtctc catcagcgag gcctggacgt tgccggcgcc
     6961 gccgatggcg tagagggaga cctccaggtg gagggggctc gtctgctgct cgtcgagcag
     7021 agccgagccg tccacctcga cgaggacggg gacacggacc cgctccccct gcttctcgac
     7081 ggggaaggcg aggagggcga aggggagggg gccgccctgc tggccgctga gcaggagcgc
     7141 ggcggtctcc atcttgaggc cgccggtgcg gagcacccgg atcggcggcc cggtcggggc
     7201 cggcgggggc gccggctcct ggagggccgg cgcggcggct gcgggcggcg ccgcctcgag
     7261 cacgagctgc ccggtggcgg cgcccgtctc ctcggccgcc ggcgggctgg ccggcgggtt
     7321 ggccggcggc ggcgcgccct gctgctgcgc cgcgaggagg gagggcagga agaggaggac
     7381 cgcggtccgc cggagagatt ccatcagcat gttccggctc cttgcttggc gttcggtctg
     7441 caactggctc gaagctctca tggtcgtctc acctggctat ctgtcgtgat cgtcgccgcg
     7501 gccctcggtc gggccggcag gctcgggggt ggcggccggt gcggcggggg atgcctcagg
     7561 cgtcaatcct ggagtgtagg aagcggtcac cgtggagacg atgttgccca actcgtcgcg
     7621 cacgcccacg gcgaccgtgt gctcgaccgg ccgcagcgcc agcttggccc ggtagccggc
     7681 gagctggccg agcgaggtca gaagctgatc gttcggtacc cggatcggca gcttgacctc
     7741 ggtgaccggc gagctccggc cctgggtgtc gcggacggcc atgaagatgg tcaccttccc
     7801 ctcgtggaac tgcccctggg ggagcagcac cagcttcgac atggggagct tcaccgtcag
     7861 ctggaccgtg ttcttcccct tctcggacac ctcctcgccg agctgcagcc cgatctccag
     7921 cgggttctcc cgcgagccga acatcagcgc cgccagcacc ttgtcggaca tcacctcggc
     7981 gctggtgcgc tcgcggtagg actcgcggtg acgcagcttg agccccggcc gcttcacctt
     8041 gacctcgatc ttgtggctct tgccgctgcg ccggtgcaag ggggtgtagc ccagcgagta
     8101 gaaggagtcc atgtcctccc gcatctcggc cagcagcacc ccggggtcga gggcgttgat
     8161 ggtggcggcg ccgccggtgg ggtcgacgat cgcatacagg gactgcgcca tgttgttcgt
     8221 ctgcagcgag tcctcggtac gggtccagag gtcgttcgtg ctggcggcgg ccgaggtcac
     8281 gttcaccgag tcggtcaccc ccagggcata gaaggtgatc cggttggcat tggcgcgcgc
     8341 tccgatcttg tccatctcct tgccgaggtc gacgtcggcg gcgttcaggc gcgagccgcc
     8401 gatgctcctg tcctgctggt tcagagtgcc gaaccggcgc tcccaggctt catagagggc
     8461 ctgcgccggc cgcagcggca cgccgccgct cagcagcagg acggccttgc gtcccggcag
     8521 gccggacagg ccgtcgacga actgctcgag ggcgctgacc atggccgcgg tcttggcacg
     8581 ctcctcctcc gcgtagagct cgatctgcgc gaggatgtcc ttggcgtcca tcaccgccat
     8641 ctccctctcc ttgtccaccc ccgctggatt ggcggaccgg tccatctgcg agatcagcat
     8701 gcgcagctcg gcgccaccgg acatgcgccc gctcgtgccc ctggcgatcc tgtcgagcgc
     8761 cgcgaccacc gtcgcgggat cgcccgtcgg catggcgcgg acctcgagcg agcccggccc
     8821 gtcgtagctc gagaggatga cgcggtcctc ggtgcgcagg cgggtcttca ggaactcgtt
     8881 gaccttgggg agggccgagt tgcgggcgcg ggcggagagg ttcgagttgt cgacgaagat
     8941 cgccagcagc agccgctgct cctccgggat ctccgcggcc tcaggcgtgg ccggcgcggc
     9001 ggtgcccagt accgtggtgg ccgcccggcc cccctcggag gcaaagaagt tggtgatctc
     9061 caccggcttg ccgtcctcga gcacctcgaa gtcgctctgc gtcaggccgt tcacccgctg
     9121 cccgctacgg tcggtgacga acacctcgac gttgacgacg ttgacctcga cggtgtcgta
     9181 gaaggtctcg tcgttctgcg ccggcgccgc cgcggcgggc gattgggccg ggaccggaag
     9241 aggaagcaac agcagggaca ccgacaagag agctgaaaat ctttggatca tcctagagct
     9301 ccttccagaa cgggccgata gagatcggac cgaaaaccat catttcttgg ctcccctgcc
     9361 ctccggcgcc gcgggctccg ccgccggacc cgcggcgggc gagtaggcgg ccgtgaccgt
     9421 ggagacgatg ttgcccagct cgtcgcgtac ccccaccgcc accgtgtgct cgagcggccg
     9481 cagcgtcagc ttggtccggt agccggcaag ctggccgcgc gccgccagca actgccggct
     9541 gggcacccgg atcggcacct tgacctcggt gatcggcgag ttccggccct cgctatcgcg
     9601 ggcggccacg aagatcgtca cccgtccctc gtggaactcc ccccggggca gcagcaccag
     9661 cttcgacagg gggagcttca ccgtcaccgg gacggcgccc ttccccttct ccgtccccgg
     9721 ccctccgagt tgcagcccga tctcgagggg gttctcccgc gagccgaaca tcagcgccgc
     9781 cagcaccttg tcggacatca cctcgctgct ggtgcgctcg cggtaggact cgcggtggcg
     9841 caccttgagc cccggccgct tcaccttgac ctcgatcttg tggctcttgc cgctgcgccg
     9901 ttgcgacggg gagtagccca gcgaatagaa ggagtccata tcctcccgca tctcggccag
     9961 cagcgtcccg gggtcgaggg cattgatggt ggcggcgccg ccggtggggg cgacgatcgc
    10021 gaacagggac tgcgccatgt tgttcgtctc cagcgtgtcc tcggtgcggg tccaggcgtc
    10081 gctggaggtg accgcggcgg agccgacgtt gaccgtgtcg gtcaccccca gggcgtagaa
    10141 ggtgatccgg ttggcgttgg cgcgcgctcc gaccttgttg atctccatgc cgaggtcgac
    10201 gtccacgccg tcgaagcgcg agccgccgat tttcttgccc tgccggttca ggtcgccgaa
    10261 ccggcgctcc caggcttcat agagggactg cgccggccgt agtggcacgc cgccgctcag
    10321 cagcaggacg gccttgcggc ccggcaggcc cgatagcccg tcgacgaact gctcgagggc
    10381 gctgaccatg gccgccgtct tgtatctctc ctcctccgcg tagaacctga tctgcgtgag
    10441 gatgtccttg gcgtccgccg cctccatctc ctcgcccccc cctgatcgtt cgatctgcga
    10501 gatcagcatg cgcagaccgg cgcccccgga catgcgaccg cccgtgcccc tggcgatttt
    10561 gtcgagcgcc gtgaccaccg tcgcgggatc gcccgtcggc atggcgcgga cctcgagcga
    10621 gcccggcccg tcgtagctcg agaggatgac gcggtcctcg gggcgcaggc gggtcttcag
    10681 gaagtcgttg accttgggga gaaccgagtt gcgggcccga gccgaaaggt tcgagttgtc
    10741 gacgaagacc gccagcagca gccgctgctc gtccgggatc tccggcgccg tgggcgtcac
    10801 ggcaggggcc gaagcccccg gcgccacggc agccgccgcg ccctccgagg cgtagaagtt
    10861 cgtgatctcc accggcctgc cgtcctcgag cacctcgaag tcgccctgcg tcaggccttt
    10921 cacccgctgg ccgctacggt cggtgacgaa aacctcgacg ttgacaacgt tgacttcgaa
    10981 agtgtcgtgg aaggtctcgt cgttctgcgc cggctgctgc gccggaaccg gaccggacag
    11041 cgccagcagg gtcaccaggg aaaggatcaa gaatcgttgg atcatccaag agctccttcc
    11101 agaacgggcc gatggagact ggaccgaaac cattcatttc gcggctccgc tgccctcccc
    11161 cgccccgggc tccgccgccg gacccgcggc gggcgagtag gcggccgtga ccgtggagac
    11221 gatgttgccc agctcgtcgc g
//
LOCUS       DASPJI010000608         6637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_39002, whole genome shotgun sequence.
ACCESSION   DASPJI010000608 DASPJI010000000
VERSION     DASPJI010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39002"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /gene="murI"
                     /locus_tag="VEL74_16950"
     CDS             <1..620
                     /gene="murI"
                     /locus_tag="VEL74_16950"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HYG64268.1"
                     /translation="QFLAGRGVKAVVVACNTASALALPRLQSDVPTWGVIDPGARRAA
                     AVSRGRVGIIATEATVRSDAYPRALRRLRPDLSILSRPCPLFVPLVEEGWTDDPVTDE
                     VARRYLAPLLEAQVDTLVLGCTHYPLLVPALERVAGPGVSLVDSAEAVAEAVAEGLAE
                     KGLEAPPGGAPHHHLCVTDAGEGFQRIARRILCDPNLTLEWVEVA"
     gene            650..1480
                     /gene="rph"
                     /locus_tag="VEL74_16955"
     CDS             650..1480
                     /gene="rph"
                     /locus_tag="VEL74_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HYG64269.1"
                     /translation="MSTPSGALRRPNGRAADALRPVHIETDVMKFAEGSVLIAAGDTK
                     VLVSASIDNRVPPFLKDSGRGWVTAEYSMLPRATHTRNGREVNQGKPSGRTSEIQRLI
                     GRALRAVIDTTAMPDRTLTLDCDVIQADAGTRTAAITGAYVAATLALAKLLLTGDIQN
                     WPITDQLAAVSVGIVGGVPLLDLEYVEDQVAEVDMNVVATAGGSIVEIQGTGEKRSFT
                     RQEMDALVDLALKGIGELAAIQNQALATTLEEVADVRSRAGQRRQAPPKPEKDIWGRP
                     "
     gene            complement(1509..3635)
                     /locus_tag="VEL74_16960"
     CDS             complement(1509..3635)
                     /locus_tag="VEL74_16960"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYG64270.1"
                     /translation="MSRYSPLLLLAVALSIPAPAAPQTSELPAPPPTRQEDFKEVLHG
                     VEIADPYRWLEDQQSPETRRWIEAQNRYTRSLLDPLPFREAIRRRLTELWRVDTTSIP
                     VEERAGRYLFFKKKAEEELATLYLRRGLDGKDEAILDPRSLSADLSVTADPMELSRDG
                     RRLIYGLRRGGEDEMELRVLDLDTRRDLPDRFPRGLYRGVAWKPDGSGFYYNLQSRET
                     GTLTRFHKLGAAPADDAEVFGRGLGRDVWAGVSEVSDDGRFVLFDVGHGWSYNDLYLQ
                     DLTAGGPPRLVTPGLKATLDVQFAGGRLLAMTDWQKPDGAPNGRIVAIDPRDPAPEKW
                     REIVPAGPDALQGFAVAGGKLFVHSLRDVTSRIRIFSLDGKPEGEVPLPGPGSVGILQ
                     ARADGGEVFFGFQSFTVPPVVYRYDVAARRVDTWAQDRPPFQPALFETRQVWYTSKDG
                     TRVPMFLVHRKGLEPNGQLPTRLYGYGGFRSSQRPFFSVTNAFWIEQGGVYALANIRG
                     GSEFGEDWHRAGMLEKKQNVFDDFIAAAEWLVANRYTNPAKLAIEGGSNGGLLVGAAL
                     TQRPDLFRAVVCRFPDLDMIGYRRFENNNPPALQEYGDASKPEHFKFLLAYSPYQKVK
                     PGTKYPAVLLTTGDNDTRVPPLQARKMTARLQAATASGIASGRPVLLLYDTVAGHAGG
                     RTAGKVIEDSTLELAFVAWQLGMEVK"
     gene            3802..4344
                     /locus_tag="VEL74_16965"
     CDS             3802..4344
                     /locus_tag="VEL74_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HYG64271.1"
                     /translation="MPVQPRLSLLHLVVLGAVALAGCVNAASRPAGAPALGCRLEAVP
                     PLTAGGPVRVRLTLTNRGESPVWALRWNTPFEGRWQGTPFTVSTGGREVPYEGPMVKR
                     GDPGREEYVEIAPGASAEATADLAEAYALAAPGRYRVEGAGGLHDLATSAAGVPRPRN
                     RHQPAPLDCAAVELTVTPPG"
     gene            complement(4354..5352)
                     /locus_tag="VEL74_16970"
     CDS             complement(4354..5352)
                     /locus_tag="VEL74_16970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYG64272.1"
                     /translation="MRSRRSRARGGRRRGRSLLWISLGLVGVGLAAALVSRRHPVFED
                     WDLDEAGSRHEPEDFEGFDPAEAPEGEALWVAGPAGNLYVRDGGENGDRGGLPMVFLH
                     SLAGNGGQWALQLDHLRRHRRAVALDLRGHGESDPADDGDYTIAGMAHDVTAVVDQLG
                     IRRFVLAGHSLGALVAIEYASRHPDRVAGLLLVDPNGDQSRVPREQIESYLAPLRLDP
                     LAELELNFRQLVLGGDPDAARWVLEDLRLTHEDAVVGALESAAVYEPLPALARYPGPR
                     LSVISEFNRLPYSLHKLLPDLPTQLLRGTGHWLMMDRPELFNQVVDEFLDEVEGRA"
     gene            5637..6101
                     /locus_tag="VEL74_16975"
     CDS             5637..6101
                     /locus_tag="VEL74_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64273.1"
                     /translation="METDLQPWRDLLQGCFDDFLDLVEPGLRQRERLETVAFLEPDEL
                     PGWPEAERRRLGLVADARIEGGRRLTILVQIEPRPGRRSVLEDRLLRYYLWLLVGRRR
                     PVRMIVVFLRGGRPGARAEAIVERHGDREVLRVPYTAFGLAGRRAGGPLTSG"
     gene            complement(6088..>6637)
                     /locus_tag="VEL74_16980"
     CDS             complement(6088..>6637)
                     /locus_tag="VEL74_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HYG64274.1"
                     /translation="DLVGKLDFSRFADAEHNVGKFTLEDIRNELLRPGRDPRPEFKTP
                     EWRADVTSVKDLEPGMVLEGRVSNVTNFGAFVDIGVHRDGLVHVSELSHRFVADPREA
                     VQVGQIVKVKVMEVDRERERISLSIKQLLAPENAGRPATGTMARGGGGGRPGSQKPGP
                     QKPQPPRQGASLDDLMRKFNRK"
BASE COUNT          892 a         2409 c         2404 g          932 t
ORIGIN      
        1 tccagttcct ggccggccgc ggcgtcaagg cggtggtggt cgcctgcaac accgcttcgg
       61 ccctggcact cccccggctg cagagcgacg tgcccacctg gggcgtcatc gaccccggcg
      121 cgcggcgcgc cgcggcggtc agccgcggcc gggtcgggat catcgccact gaggcaacgg
      181 tccgctccga cgcctacccg cgggcgctgc ggcggctgcg gccggacctc tcgatcctga
      241 gccgcccctg cccgctgttc gtgccgctgg tcgaggaggg gtggaccgac gatccggtga
      301 ctgacgaggt cgcccgccgc tacctggcgc ccctgctcga ggcccaggtc gatacgctgg
      361 tgctgggctg cacccactat ccgctgctgg tgccggcgct ggagcgcgtc gccgggccgg
      421 gggtgtcgct ggtcgactcc gccgaggcgg tggccgaggc ggtggccgaa ggcctcgccg
      481 agaaggggct ggaggcgccg cccggcggcg ctccccacca ccacctctgc gtcaccgacg
      541 ccggcgaggg gttccagcgc atcgcccggc gcattctctg cgacccgaac ctcaccctgg
      601 aatgggtcga agtggcgtag tccgggtcat cccggtctag aatcccggca tgagcacacc
      661 ttctggagcc ctccgccgcc ccaacggccg cgccgccgat gccctgcgcc cggtccatat
      721 cgaaaccgac gtcatgaagt tcgccgaggg ctccgtgctc atcgcggccg gtgacaccaa
      781 ggtactggtc tcggccagca tcgacaaccg ggtgccccct ttcctcaagg actcgggccg
      841 cggctgggtg accgccgagt actccatgct gccgcgggcg acccacacgc gcaacggccg
      901 cgaggtgaac cagggcaagc cctcggggcg cacctccgag atccagcgcc tgatcggccg
      961 cgcgttgcgg gcggtcatcg acacgacggc catgccggac cgcaccctca cactggactg
     1021 cgacgtgatc caggccgacg ccggcacccg caccgccgcc atcaccggcg cctacgtggc
     1081 cgccaccctg gccctcgcca agctgctgct gaccggcgac atccagaact ggccgatcac
     1141 cgaccagctc gccgcggtgt cggtggggat cgtcggcggc gtgccgctgc tcgacctcga
     1201 gtacgtcgag gaccaagtgg ccgaggtcga catgaacgtg gtcgccaccg ccggcggctc
     1261 gatcgtcgaa atccagggca cgggggagaa gcgcagcttc acccggcagg agatggacgc
     1321 gctggtggac ctggccttga aggggatcgg cgagctcgcc gcgatccaga accaggcgct
     1381 ggcgaccacc ctggaggagg tcgccgacgt ccgctcccgc gccggccagc gccgccaagc
     1441 gccgcccaag cccgagaagg acatctgggg ccggccctga gcggccggcc cggccccacg
     1501 ggttccccct acttcacctc catcccgagc tgccaggcga cgaaggccag ctcgagcgtg
     1561 ctgtcctcga tgaccttgcc cgcggtgcgg ccgccggcgt ggccggccac ggtgtcgtag
     1621 aggaggagga ccgggcggcc cgaggcgatg cctgaggcgg tagcggcctg gaggcgggcc
     1681 gtcatcttgc gggcctggag gggcggcacg cgtgtgtcgt tgtcccccgt ggtcagcagc
     1741 accgcggggt acttcgtccc cggcttcacc ttctggtacg gcgagtaggc gagcaggaac
     1801 ttgaagtgct ccggcttcga ggcgtcgccg tactcctgga gcgccggcgg gttgttgttc
     1861 tcgaaccggc ggtagccgat catgtccagg tccgggaagc ggcagactac cgcccggaag
     1921 aggtccggcc gctgggtgag cgccgccccc accagcaggc cgccgttgct ccccccctcg
     1981 atcgccagct tcgccgggtt ggtgtagcgg ttggcgacga gccactccgc cgcggcgatg
     2041 aagtcgtcga agacgttctg cttcttctcc agcatccccg cccggtgcca gtcctcgccg
     2101 aactcgctgc cgccgcggat gttggcgagc gcgtagactc ccccctgctc gatccagaag
     2161 gcattggtga cgctgaagaa cggccgctgg ctggaccgga agccgccgta gccgtagagg
     2221 cgcgtgggga gctggccgtt cggctcgagc cccttccggt gcaccaggaa catcggcacc
     2281 cgggtgccgt ccttggaggt gtaccagacc tggcgcgtct cgaagagggc gggctggaag
     2341 ggggggcggt cctgcgccca ggtgtcgacc cggcgcgccg ccacgtcgta ccggtagacc
     2401 accggcggca cggtgaacga ctggaagccg aagaacacct cgccgccgtc cgcgcgcgcc
     2461 tgcaggatgc ctaccgagcc cggtcccggc agcggcacct ccccctccgg cttgccatcc
     2521 agcgagaaga tgcggatgcg cgaggtcacg tcgcgcaggg agtggacgaa cagcttgccg
     2581 ccggcgaccg cgaatccctg gagcgcgtcg gggccagcgg gcacgatctc gcgccacttc
     2641 tccggcgccg ggtcgcgtgg gtcgatggcg acgatgcggc cgttgggggc gccgtccggc
     2701 ttctgccagt cggtcatggc gaggagccgg ccgcccgcga actggacgtc cagggtggcc
     2761 ttgaggccgg gggtcaccag cctgggcggg ccgccggcgg tgaggtcctg gaggtacagg
     2821 tcgttgtagc tccagccgtg cccgacgtcg aagaggacga accggccgtc gtccgagacc
     2881 tcggacacgc cggcccagac gtcgcggccg aggccgcggc cgaagacctc ggcgtcgtcc
     2941 gccggcgcgg cgcccagctt gtggaagcgg gtgagggtgc cggtctcgcg gctctgcagg
     3001 ttgtagtaga agccgctgcc gtccggcttc caggccacgc cgcggtagag gccgcggggg
     3061 aagcggtcgg gcaggtcgcg gcgggtgtcg aggtccagca cccgcagctc catctcgtcc
     3121 tcgccgccgc gccgcaggcc gtagatcagc cgccggccgt cgcgcgagag ctccatggga
     3181 tcggcggtga cgctcaggtc ggcgctcagg ctgcggggat cgaggatcgc ctcgtccttg
     3241 ccgtcgagcc cccggcgcag gtagagggtc gcgagctcct cctccgcctt cttcttgaag
     3301 aagaggtagc ggccggcccg ctcctccacc gggatgctgg tggtgtccac ccgccagagc
     3361 tcggtcagcc ggcgccggat ggcctcgcgg aaggggaggg ggtcgagcag cgagcgggtg
     3421 taccggttct gcgcctcgat ccaccgccgc gtctccgggc tctgctggtc ctcgagccag
     3481 cggtaggggt cggcgatctc gaccccgtgc agaacctcct tgaaatcctc ctgccgcgtc
     3541 ggcggcggcg cgggcagctc cgaagtctgc ggagccgccg gagcggggat cgagagggcg
     3601 acggcgagga gcagcagcgg cgagtatcgg gacatgaagc ctccttggga atcactgtac
     3661 cacccgactt agacgggagc cgcctcactt cagttcgcgg cgcgcttcag ccccctccgt
     3721 gtaccttttc ttgctggagc actccggaca gattcacccg ctagcccgct tcccggggct
     3781 ggagggcagg aggatcccgt catgcccgtc cagccccgtc tctcgctcct ccacctcgtc
     3841 gtcctcgggg ccgtcgccct ggcgggctgc gtgaacgcgg cttcgcggcc ggccggcgct
     3901 ccggccctcg gctgccgcct cgaagcggtg ccgccgctca ccgccggcgg gccggtacgg
     3961 gtgcgcctga cgctcaccaa ccggggggag tccccggtct gggcgctacg ctggaacacc
     4021 ccgttcgagg ggaggtggca ggggacgccc ttcaccgtca gcaccggcgg ccgggaggtg
     4081 ccctacgagg gcccgatggt caagcgtggc gatccggggc gggaggagta cgtggagatc
     4141 gcgcccggag cgtcggccga ggccacggcg gacctcgccg aggcctacgc gctggccgcg
     4201 ccgggccgct atcgggtgga aggcgccggg ggcctccacg acctggccac ctccgccgcc
     4261 ggcgtccccc gcccacggaa ccgccaccag ccggcccctc tggactgcgc cgccgtcgag
     4321 ctgacggtca caccgccagg gtgacgagcg gggctacgcc cgcccctcca cctcgtcgag
     4381 gaactcgtcc accacctggt tgaacagctc cggccggtcc atcatcagcc agtggccggt
     4441 gccgcgcagg agctgggtgg gcaggtcggg cagcagcttg tggaggctgt acgggaggcg
     4501 gttgaactcg ctgatcaccg acagcctggg gcccgggtag cgcgccaggg ccggcagcgg
     4561 ctcgtagacg gcggcgctct ccagcgcccc gacgacggcg tcctcgtggg tgaggcggag
     4621 atcctcgagc acccagcggg cggcgtccgg gtcgcccccc aggacgagct gccggaagtt
     4681 gagctccagc tccgccaggg ggtccagccg caggggggcc aggtaggact cgatctgctc
     4741 ccgcggcacc cggctctggt cgccgttggg gtcgaccagc agcagcccgg ccacgcggtc
     4801 ggggtgccgg gaggcgtact cgatggcgac cagggcgccg aggctgtggc cggcgaggac
     4861 gaagcgccga atcccgagct gatcgaccac cgcggtcacg tcgtgggcca tgcccgcgat
     4921 ggtgtagtcg ccgtcgtcgg ccggatcgga ctcgccatgc ccgcgcaggt cgagggccac
     4981 ggcgcgccgg tggcggcgga ggtggtcgag ctgcagcgcc cactggccgc cgttgcccgc
     5041 caggctgtgg aggaagacca tgggcagccc gccacggtca ccgttctcgc cgccatcccg
     5101 cacgtagagg ttgccggccg gcccggcgac ccagagggcc tcgccctccg gcgcctcggc
     5161 cggatcgaag ccctcgaagt cctccggctc gtgccgggac ccggcctcgt cgaggtccca
     5221 gtcctcgaag accggatggc gccgcgagac gagcgccgcg gccagaccga cccccaccag
     5281 ccccagggag atccacagaa ggctccgccc ccgcctcctc ccgccgcgag ctctggaacg
     5341 cctcgacctc atgcgcctca cctcctcgaa atgctccggg gccaacctaa cacgaccggc
     5401 cgttccggcg ccaccgggag gtggggtgcg gggccggtct gtccggcgca ggcgactggc
     5461 agcgtggcag ggagggaggg ggagcgcaca cgaaggagcc gagcaggaca gaacggcccc
     5521 gcaccccgcc ggcggagcaa ggcaaagatt tttgagcgat ttttcccgcg ttttccgtct
     5581 ttcccctggc gaaggggctg cccactcccg ggccgccccg ttcgtgagga gagaccgtgg
     5641 agaccgacct tcaaccgtgg agagacctgc tgcagggatg cttcgacgac ttcctggacc
     5701 tggtggagcc ggggctgcgg cagcgcgagc gcctggagac cgtcgccttc ctggagccgg
     5761 acgagctacc cggctggccc gaggccgagc gccggcggct cggcctcgtc gccgacgccc
     5821 ggatcgaggg ggggcgccgg ctgaccatcc tggtgcagat cgagccccgg cccgggcgcc
     5881 ggagcgtcct ggaggaccgg ctgctgcgct attacctgtg gctcctcgtc gggcgccgcc
     5941 ggccggtgcg catgatcgtc gtcttcctgc gcggcggccg gccgggcgcc cgcgccgaag
     6001 ccatcgtcga gcggcacggc gaccgcgagg tgctgcgcgt cccctacacc gccttcggcc
     6061 tcgccggacg ccgcgccggg gggcccctca cttccggttg aacttcctca tcaggtcgtc
     6121 gaggctggcc ccctgccgcg gcggctgcgg tttctggggg cccggcttct gggagccggg
     6181 ccgcccaccg ccgccgcccc gtgccatcgt gccggtggcg ggccgccccg cgttctccgg
     6241 cgccaggagc tgcttgatcg acaggctgat ccgctcgcgc tcgcggtcca cctccatcac
     6301 cttgaccttg acgatctgcc ccacctggac ggcctcgcgc gggtcggcca cgaagcggtg
     6361 cgacagctcc gagacgtgca ccaggccgtc gcggtggacg ccgatgtcga cgaaggcgcc
     6421 gaagttggtg acgttggaga ccctgccctc gagcaccatc cccggctcca ggtccttgac
     6481 gctggtgacg tcggcgcgcc actcgggggt cttgaactcg gggcgggggt cgcggcccgg
     6541 gcggagcagc tcgttgcgga tgtcctccag ggtgaacttc cccacgttgt gctcggcgtc
     6601 ggcgaaacgg ctgaagtcga gcttgcccac caggtcg
//
LOCUS       DASPJI010000609         9638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_720837, whole genome shotgun sequence.
ACCESSION   DASPJI010000609 DASPJI010000000
VERSION     DASPJI010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9638)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9638
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720837"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2251)
                     /locus_tag="VEL74_16985"
     CDS             complement(<1..2251)
                     /locus_tag="VEL74_16985"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYG64275.1"
                     /translation="MEGFRLSPQQERLWRLAGSAEAAAGYRAWCVAAVEGPLDRGALE
                     RALELAVERNELLRTSFRTVPGLAAPLQVPSPPGPLSRLPYPLPGRGGEEEALSALLA
                     QPFDLAGGPLLRWSVLPLSPERHLLLLALPALLADTAGLDNLLGEIARCYAAAVAGNP
                     LQDEPVQYVDLSEWQHELLESEDTQAGRDFWRRQPETPETPELPLVIPASAGPWAPQS
                     LRVPLPTDLPRLAEAWGVPVGQALLAGWQALLWHLVGRPESLALAVTFAGRKFEELRE
                     APGLFARDLPIQARLEPGMLLRNLAGQLHRDLSDVAEWQEYFTPAVGARDLPFAFEER
                     PPVRRYQAGLLAFTVLRRDASHGRALARLIAEPGAVDLHYDSFRLPDAAARLLVERLA
                     TLLTSAANDPDAVLADLELVGPEERRLLLEDWNDTRTAFPGESLIHRLIENQAAKTPE
                     RIAVESDSGRWTYADLEARANDLARRLSVFGIGPEARVAVCLDRSLDMVAALLAIWKT
                     GGAYIPLDPAYPHERLRLMLDDAHPALLLTGPGALPELAPPSLPILDLGSTEEAGPGA
                     VGAAQATGRARGREVEAHPKELSRAEPTQAQPAGVPTSSDQLAYILYTSGSTGRPKGV
                     MVSHQAIANRLLWMAAAFPLAEDDAVLQKTPFGFDASIWELFLPLLTGSRLVMAPPGA
                     HRDPAALTRSVAAHGITVLQLVPSLLGPFLDADGLSGCRSLRRLFCGGELLSPALRDL
                     TFARLARPDI"
     gene            complement(2264..9316)
                     /locus_tag="VEL74_16990"
     CDS             complement(2264..9316)
                     /locus_tag="VEL74_16990"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYG64276.1"
                     /translation="MSNESPLTGMEIAIVGMACRFPGARNVEEFWANLRDGVESITVL
                     TREQLEESREEPESIDSPDYVPVARLIEGEDLFDAQLFNCSPREAEIIDPQQRILLEC
                     AWEAMESAGYDSERFPGLVGVYAGSKLNGYLWNLYSNPRIIQSIGDFQAQIANDKDYV
                     ATRIAYKLSLGGPAVTVQSACSTSLVAVHLACQGLLAGESDMALAGGVSVRLPQRLGY
                     TYREGEILSPEGRVRAFDAQAQGTIFGNGMGMVVLRRLEDALADGDRIRAVIKGSAVN
                     NDAAVKVGFTAPGVDGQVRVVRAAHLTAQVAPETISYIEAHGTGTLLGDPIEVRALTQ
                     AFRAGTDRRGFCAIGSVKTNIGHLGAAAGVAGLIKAVLALENGAIPPSLLFERPNPQI
                     DFESSPFFVNTELRPWTRNGVPRRAGVSAFGIGGTNAHVVLEEAPAAPPVAPAREWQL
                     LVLSAKTPTALDAVTARLREHLERHPEQELADVAFTLETGRRPLEHRRVLVCREHAEA
                     VEVLAGGDAARMPSGVPEGPNPPVVFLFSGQGAQYAGMGRDLYRDEPVFRDEIDRCAA
                     LLQPRLGLDLRDLLYPASGTPEDHEEANRRLGRTAVTQPALFTIEWALARLWMSWGIR
                     PEAMIGHSIGEYVAACVAGVFSLEDALVLVAERGRLMEGLPAGAMLSVPLPEAEVEAL
                     LGRQLSLAAVNAPGRCVVSGPEEAVAALEKQLAARGVACRRLHTSHAFHSGMMEPILP
                     PFVERVAAMALHPPQIPYISNVTGGWIREAEATDPEYWARHLRGTVRFGDGLAELFQE
                     RTRVMLEVGPGQNLTTFCRQHPGRGVSRVIVSSLRHPREEHHDTVFLLRSLGALWLAG
                     AAPDWMAFHAGETRRRVTLPTYPFERKRYWIAPGNTAAAFGGDQAALRRKSDVADFFH
                     VPSWRPSPPPAPPRDGGPASWLVFADGCGLADALVARLRAEGRRADVVHAGAGFARLG
                     DGAWEIDPGRREDYGALLAALRAEGAAPDAVAHLWGVTPAEGAAEYRETQTLGFYSLL
                     WLAQALAGESGGVRLGVATTGVQRVWGTEALTPEKASVLGPCKVVPQENPRLSTVAVD
                     VVLPPAGASLSGLAALADRLWAEIAAPAGERVERVVALRDGIRWVQEWNAVRLEQAAE
                     ERLPLREQGVYLVTGGLGGLGLVFADYLARSWRARLVLVGRSALPPREEWEALAASLP
                     EDDTTGGRLRKLLALEAAGGEVLAASADVADEAAMRNVVTEAVARFGAVHGVIHAAGV
                     AGGGVVQLKTDEVAERVLAPKILGTRALAAALAGMPLDFSLLCSSTIGVLGGFGQVDY
                     CGANSFLDAWAHAAASRGERVVSVNWGAWDEVGMAVQTQLPRGARQAARTWREAPLPL
                     PDTPPLHPLIDECLSRTEDRAVFLTRFDERRHWVVAEHRVAGTSAVPGTGYLEMARAA
                     FQHVTGETAAEVRDVLFLAPVMVEEGEPREVRISLERGGEAWDFKVESRGDDGWQENA
                     RGRVVPLNGAPTQAASIAEIQARCTGRDEVIPAGVKPENEGQGLVYWGPRWQSLRRVQ
                     ASEGEAVALLELPAEFAGDLEDGLVLHPALLDVATALSVFVAGGQESYLPLSYRRLRV
                     RRSIPGRVWSHVRTHDGASRETLTADITLLDDAGEPVVEIEQFSLKRVGEAAGRFRRP
                     AAKTAAANPLGVGGIRPAEGVEALRRLLSCPLGPQVAVSAKDLSALARQAVTTPDAVG
                     GAGAGMAEGPTLALQEPMARYPRPDLKVPYAPPTGVTESLLVDIWQRVLGLEQVGIHD
                     NFFELGGDSVMGIHVATRAAEAGLQVTPEQLFQYQTVAELAAALPETTGAPVPAMAEP
                     ALEETESGERFPLAHLDAETLERILAGREVEDLYPLSPLQQGFLFHSLSSPESGLYLE
                     QMSCTLTGPLHVELMEQSWQHALDRHPVLRTSFLWEDLERPLQAVSPRVAMPLLVEDW
                     RNLPEEEQRQRFEVARQADRERPFRLSEAPLMRLHLFRMADDVHRLLWTHHHLLMDGW
                     SSPLLAQEVLMAYEQLRRGGELPPAPARRFRHYIEWLEGQDLGRAEIFWRQALAGFSQ
                     PTQVRADRPGDGSLGLSTDFERLDFLVPAETAASVQAVARQGQFTLNNVAQGLWALLL
                     GRAAGEDDVIFGITVSSRPSSIPGIESMVGLFINALPIRVRIAPEEPLAGWLKGLQEL
                     QGALLQHAHTPLVDIQRWSEIPGDQPLFESIYEFWNFPLPSGEDDRLSVHMSDPHYDV
                     ATNYPLSLRVIPGEGLQMQITYDRRRFEVSTIERLRRDLEIALGAVATLGERPEARVG
                     DLVAAVDEAEREERRTQAAEVSSVAQEKLRQRTRRRGTPTGAGV"
     gene            complement(9338..>9638)
                     /locus_tag="VEL74_16995"
     CDS             complement(9338..>9638)
                     /locus_tag="VEL74_16995"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYG64277.1"
                     /translation="LQDVLGLDRIGLHDNFFEQGGNSLLLVRAHARLSELIGGDLQVV
                     DLFSHPTVALLAAHAGRQVATVSVVSAARQSDERMDKVEAGQSRLQKLRQRRQQG"
BASE COUNT         1428 a         3483 c         3484 g         1243 t
ORIGIN      
        1 cgatatcggg acgggcgaga cgggcgaagg tcagatcgcg aagggcaggc gagaggagct
       61 cgccgccgca gaagaggcgg cgtagcgaac ggcagccgga gaggccgtcg gcatccagga
      121 aggggcccag gagcgaaggg accagttgga gcacggtgat gccgtgagcg gcgacagatc
      181 gagtaagagc ggccgggtcc cggtgggcgc ctggaggcgc catgacgaga cgggagccgg
      241 tgaggagcgg gaggaacagc tcccagatgg aggcgtcgaa gccgaagggg gtcttctgga
      301 ggacggcgtc gtcttcggcg agcgggaagg cggcggccat ccagagtaga cggttggcga
      361 tggcctggtg cgacaccatg acgcccttcg ggcgtccggt ggagccggag gtgtagagga
      421 tgtaggcgag ttggtcggag gaggtgggga ctccggccgg ctgggcctgg gtcggctcgg
      481 ccctgctcag ctccttcggg tgcgcctcca cctccctccc ccgggcgcgt ccagtcgcct
      541 gcgccgcgcc gaccgcccca ggcccagcct cctccgtgga acccaggtcc aggataggga
      601 gtgacggcgg ggcgagctcg gggagcgctc ccggaccggt gaggagaaga gcgggatggg
      661 cgtcgtccag catcaggcgc aggcgctcgt ggggataggc gggatcgagc gggatgtagg
      721 cgccccccgt cttccagatc gccaggagag ccgccaccat gtccagggaa cgatcgagac
      781 agacggcaac gcgggcctcg ggaccgatac cgaagacgct gagacggcga gcgagatcgt
      841 tggcgcgggc ctcgaggtcc gcataggtcc agcgcccgga gtcagattcg accgcgatgc
      901 gctccggcgt cttcgcggcc tggttctcga tcaggcggtg gatcaacgat tcgccgggaa
      961 aggcggtgcg ggtgtcgttc caatcttcga ggagcaggcg gcgctcctcg ggacctacca
     1021 gttcgagatc cgccaggacg gcatcagggt cattggcggc gctggtgagc agagtggcca
     1081 agcgctcgac caggaggcgg gccgccgcat ccgggagccg gaacgagtcg tagtggagat
     1141 ccacggctcc aggctcggcg atcagccggg cgagagcacg gccatggctg gcgtcgcgcc
     1201 ggagcacggt gaaggccagg agaccggcct ggtagcggcg gaccggcgga cgctcctcga
     1261 aagcaaaagg caggtcccgg gctccgaccg caggggtgaa gtactcctgc cactcggcca
     1321 cgtcggacag gtcacggtgg agctgaccgg cgagattgcg cagcagcatt ccgggctcga
     1381 gacgcgcctg gatgggcagg tcgcgggcga acagccccgg cgcctcccgc agctcctcga
     1441 acttgcggcc ggcgaaggtc accgccagcg ccaggctctc gggccggccc acgagatgcc
     1501 agagcagcgc ctgccaaccg gccagcaggg cctgccctac cggcacgccc caggcctcgg
     1561 ccagacgcgg caggtccgtg ggcaacggca cccgcaggct ctgcggcgcc cagggcccgg
     1621 cggaagccgg gatcaccagg ggcagctcgg gggtctccgg cgtctcgggc tggcggcgcc
     1681 agaaatcacg gccggcctgc gtgtcctccg attccagcag ctcgtgctgc cactcggaga
     1741 gatcgacgta ttggaccggc tcgtcttgaa gcgggttgcc ggcgaccgcc gcggcatagc
     1801 agcgggcgat ctctcccaac aggttgtcga gccccgccgt gtcggcgagc agggcgggga
     1861 gggcgaggag cagcaggtgg cgctccgggc tcaggggcag caccgaccag cgcaggagcg
     1921 gaccgcccgc caggtcgaac ggctgcgcca gcagggccga aagggcctcc tcctcccccc
     1981 ctctcccggg gagggggtag ggaaggcggg agagggggcc ggggggtgag gggacctgca
     2041 acggcgcagc gagccccggc acggtccgga atgaggtccg cagcagctcg ttgcgctcca
     2101 cggccagctc cagtgccctc tccagggctc cccggtccag ggggccttcg accgcggcga
     2161 cgcaccaggc tcggtaacct gcggccgcct cggcggagcc ggcgaggcgc cagaggcgct
     2221 cctgttgcgg cgacagccgg aagccttcca acccctccac cctctagacc cccgctcccg
     2281 tcggcgtgcc gcgccggcgg gtccgctgcc gcagcttctc ctgggcaacg ctggaaacct
     2341 ccgccgcctg ggtccgccgc tcctcacgtt ccgcctcgtc caccgcggcg acgagatcgc
     2401 ccacccgcgc ctccggccgc tcaccgaggg tggccacggc gcccagggcg atctccaggt
     2461 cgcgccgcag ccgctcgatc gtagacacct cgaagcgccg gcggtcgtag gtgatctgca
     2521 tctgtagccc ctcgccgggg atgacccgca ggctcagcgg atagttggtg gccacgtcgt
     2581 agtggggatc cgacatgtgc acggacagcc ggtcgtcctc ccccgacggc agcgggaagt
     2641 tccagaactc gtagatgctc tcgaacagcg gctgatcgcc cgggatctcg ctccacctct
     2701 ggatatccac cagcggcgtg tgggcgtgct gcaggagagc cccctgcagc tcctgtagac
     2761 ccttgagcca gccggccaga ggctcctccg gagcgatccg gacccgtatc ggcagggcgt
     2821 tgatgaacag gcccaccatg gattcgatac ctggaatgga cgaggggcgg ctcgacacgg
     2881 tgatgccgaa gatcacgtcg tcttcgccgg cggcgcggcc caggagcagg gcccagaggc
     2941 cctgggcgac gttgttcaac gtgaattggc cctgccgcgc caccgcctgc accgaagccg
     3001 cggtctccgc cggtaccagg aagtccaggc gctcgaagtc tgtggagagg ccgagcgagc
     3061 cgtcaccggg gcggtcggcg cgcacctgcg tcggctggct gaacccggca agagcctgcc
     3121 gccagaagat ctcggcgcgg cccaggtcct gcccctccag ccactcgatg tagtgccgga
     3181 accggcgcgc cggagccggc ggcagctcgc cgccgcggcg gagctgctcg taggccatta
     3241 ggacctcctg cgccagcagg ggcgacgacc agccgtccat cagcaggtgg tggtgggtcc
     3301 acagcagccg gtggacgtca tcggccatgc ggaagaggtg gaggcgcatg agcggcgcct
     3361 cggagagccg gaacggccgc tcccggtcgg cctgccgggc cacctcgaag cgctgccgct
     3421 gctcctcctc cggcaggttc cgccagtcct ccaccaggag cggcatggcg acgcgcgggc
     3481 tcaccgcctg gagcggccgc tccagatcct cccagaggaa cgaggtgcgc agcaccgggt
     3541 ggcgatccag cgcgtgctgc caggactgct ccatgagctc gacgtgcagg gggccggtca
     3601 gggtgcagct catctgctcc aggtagaggc ccgactcggg cgagctcagg ctgtggaaga
     3661 ggaagccctg ctgcagcggc gacaggggat agaggtcctc cacctcgcgg ccggcgagga
     3721 tccgctccag ggtctcggcg tccaggtggg ccagggggaa gcgctcccct gattccgtct
     3781 cctccagcgc cggctcggcc atggccggca ccggcgcccc ggtagtctcc ggcagcgccg
     3841 ccgccagctc ggccaccgtc tggtactgga agagctgctc gggggtgacc tgaagccccg
     3901 cctccgccgc ccgggtggcg acgtggatgc ccatcaccga gtcgccgccc agctcgaaga
     3961 agttgtcgtg gatgcccacc tgctcgagcc cgagcacccg ctgccagatg tccaccagca
     4021 gcgactcggt gacgccggtg ggcggagcat agggaacctt gaggtccgga cgggggtagc
     4081 gggccatcgg ctcctggagg gcgagggtag ggccctccgc catgcccgct ccggcgccgc
     4141 ctaccgcgtc cggcgttgtg acggcctgac gcgccagcgc cgacaggtcc ttggccgaga
     4201 ccgcgacctg ggggcccagg gggcaggaga gcagccggcg cagagcctcc accccctcgg
     4261 ccggacggat gcccccgacc cccagcggat tggccgccgc ggttttcgcc gccggccgcc
     4321 ggaagcgtcc ggccgcctcg cccacccgct tcagcgagaa ctgctcgatc tccaccaccg
     4381 gctcgccggc gtcgtccagc agggtgatgt ccgccgtcag cgtctcccgg ctggccccgt
     4441 cgtgggtgcg cacgtggctc cagacccgtc cggggatcga ccgccgcacc cgcagccgcc
     4501 ggtaggacag cggcaggtag ctctcctggc cgcccgccac gaagacgctg agggcggtag
     4561 cgacgtccag aagcgccggg tggaggacca gaccgtcctc caggtctccg gcgaactcgg
     4621 cgggtagctc gaggagggcg acggcttctc cctcgctcgc ctggacgcgg cgcaggctct
     4681 gccagcgcgg cccccagtag accagtccct gcccttcgtt ctcgggcttg acgccggcgg
     4741 ggatcacctc gtctcgccca gtgcagcggg cctggatctc ggcgatcgag gcggcctgcg
     4801 tgggagcgcc gttcaggggg accacacggc cgcgggcgtt ctcctgccaa ccgtcgtctc
     4861 cccggctctc caccttgaaa tcccaggcct ctcccccccg ttcgagagag atgcgcacct
     4921 cccgcggctc gccctcctcc accatcaccg gtgccaggaa gagcacgtcg cgcacctcgg
     4981 ctgccgtctc gccggtgacg tgctggaacg ccgcccgggc catctccaga taccccgtgc
     5041 cgggcacggc gctggtgccg gccacccggt gctcggcgac gacccagtgc cggcgctcgt
     5101 cgaagcgggt gaggaagacc gcccggtcct ccgtccggga caggcactcg tcgatcagcg
     5161 gatggagcgg cggggtgtcg ggaagtggca gcggcgcctc ccgccaggtc cgggccgcct
     5221 gccgcgcgcc gcgcgggagc tgcgtctgga ccgccatccc cacctcgtcc caggctcccc
     5281 agttcaccga caccacccgc tcgccgcggc tggcagccgc gtgggcccat gcgtccagga
     5341 aggagttggc gccgcaatag tccacctgac cgaagccgcc gaggacgccg atggtggacg
     5401 agcagaggag agagaagtcc aggggcatgc cggccagggc cgccgccagg gctcgcgtgc
     5461 ccaggatctt cggtgccagc acccgctcgg ccacctcgtc ggtcttgagc tggaccacgc
     5521 cgccccccgc cacacccgcg gcatggatga cgccgtgaac ggcgccgaag cgggccacgg
     5581 cctcggtcac cacgttgcgc atggccgcct cgtcggcgac gtcggcgctc gccgccagca
     5641 cctcgccgcc ggccgcttcg agggccagga gcttgcgcag ccgcccgccg gtggtgtcgt
     5701 cctccggcaa cgaggccgcg agcgcctccc actcctcgcg tggcggcagc gccgagcgcc
     5761 ctactaggac cagacgggcg cgccaggacc gcgccaggta gtcggcgaag accagaccga
     5821 gaccgccgag gccgccggtg acgaggtaga ccccctgctc gcgcaacggc aatcgctcct
     5881 ccgccgcctg ctcgagccgc accgcgttcc actcctgcac ccagcggatg ccgtcgcgca
     5941 gggccaccac ccgctcgacc cgctcgccgg ccggcgcggc gatctccgcc cacagacggt
     6001 ccgcgagagc cgcgaggccg gagagcgacg cccccgcagg cggcaggacc acgtccaccg
     6061 ccaccgtcga caggcgcggg ttctcctgcg gcaccacctt gcagggaccg agcaccgagg
     6121 ccttctccgg cgtcagggcc tccgtccccc acacccgctg gacccccgtg gtggcgacac
     6181 cgaggcggac cccaccgctc tcgccggcga gtgcctgggc cagccagagc aagctgtaga
     6241 accccaacgt ctgggtctct cgatactcgg ctgccccctc cgccggtgtc acgccccaca
     6301 gatgggcgac ggcgtccggc gccgccccct cggcgcgcaa ggcggcgagg agggcgccgt
     6361 agtcctcgcg gcggccgggg tcgatctccc aggcgccatc ccccaggcgc gcgaagccgg
     6421 cgccggcgtg gaccacgtcg gcccggcgcc cctcggcgcg caatcgggcc accagggcat
     6481 cggccagacc gcagccgtcg gcgaagacca gccaggaggc ggggccgcca tcccgtggcg
     6541 gcgccggagg gggggaagga cgccaggagg ggacgtggaa gaagtcggcc acgtcgctct
     6601 tgcggcgcag cgcggcctga tcgccaccga acgcggccgc ggtgttgccc ggcgcgatcc
     6661 agtagcgctt acgctcgaag ggataggtgg gcagcgtcac ccgccgccgc gtctcgccgg
     6721 catggaacgc catccagtcc ggcgccgcgc ccgccagcca gagcgctccc agggagcgca
     6781 ggaggaagac cgtgtcgtgg tgctcctccc gcgggtggcg aagcgatgag acgatcaccc
     6841 gcgacacccc gcggccgggg tgctggcggc agaaggtggt cagattctgc ccgggaccca
     6901 cctccagcat cacccgtgtc cgctcctgga acagctcggc gaggccgtcg ccgaagcgga
     6961 cggtcccccg caggtgacgg gcccagtatt cgggatcggt ggcctcggcc tcccggatcc
     7021 agccgccggt gacattggag atgtacggga tctggggcgg atggagcgcc atggccgcga
     7081 cccgctccac gaacggcggc aggatgggct ccatcatgcc ggagtggaag gcgtgggagg
     7141 tgtggagccg ccggcaggcg acgccgcggg cggcgagctg tttctcgagc gccgccacgg
     7201 cctcctcggg gccggagacc acgcaccgcc ccggcgcgtt gaccgccgcc agcgagagct
     7261 ggcggcccag cagggcctcc acctcggcct ctggcagcgg cacgctgagc atcgcccccg
     7321 ccggcagccc ctccatcagc cggccgcgtt cggccaccag gaccagggcg tcctccagcg
     7381 agaagacgcc ggcgacgcag gccgccacgt actcgccgat gctgtggccg atcatcgcct
     7441 cgggccggat gccccaggac atccagagcc gcgccagcgc ccactcgatg gtgaacagcg
     7501 ccggctgggt cacggcagtg cggccgaggc gccggttcgc ctcctcgtgg tcctcagggg
     7561 ttccggacgc cggatagagc aggtcccgca ggtcgaggcc gagccgcggc tggagcagcg
     7621 cggcgcagcg gtcgatctcg tcacggaaca ccggctcgtc gcggtagaga tccctcccca
     7681 tgccggcgta ctgcgccccc tggcccgaga acaggaagac caccggcggg ttcggcccct
     7741 ccgggacgcc cgacggcata cgggccgcgt cgccacccgc caggacctcc accgcctcgg
     7801 cgtgctcgcg gcagaccagg acacgccggt gctccagggg ccggcgcccg gtctccaggg
     7861 tgaaggcgac gtccgccagc tcctgctcgg gatggcgctc gaggtgctcg cgcagccggg
     7921 cagtcaccgc gtccagggcc gtgggggtct tggccgacag caccaggagc tgccactccc
     7981 gggcgggggc caccggcggc gctgccggcg cctcctccag caccacgtgg gcgttggtgc
     8041 cgccgatgcc gaaggcgctg acgcccgccc ggcgcggcac gccgttgcgg gtccacggcc
     8101 gcagctccgt gttgacgaag aaggggctgc tctcgaagtc gatctgcggg ttgggccgct
     8161 cgaacagcag gctgggcggg atcgccccgt tctccagcgc cagcaccgcc ttgatgaggc
     8221 cggccacgcc cgccgccgcg cccaggtgcc cgatgttcgt cttcaccgat ccgatggcgc
     8281 agaagccgcg gcggtcggtg ccggcgcgga aggcctgcgt gagggcccgc acctcgatcg
     8341 gatcgcccag cagcgtgccg gtgccgtggg cctcgatgta ggagatggtt tcgggggcca
     8401 cctgtgcggt gaggtgggcg gcgcgcacca cccggacctg gccgtcgacg cccggcgcgg
     8461 tgaagcccac cttgaccgcg gcgtcgttgt tcaccgccga ccccttgatc acggcgcgga
     8521 tccggtcacc gtcggccagg gcgtcctcca ggcgccggag caccaccatg cccatgccgt
     8581 tgccgaagat ggtcccctgg gcctgggcgt cgaaggcacg gacgcgccct tcgggagaca
     8641 ggatctcccc ctcgcggtag gtgtagccga ggcgctgggg caggcggacg gagaccccgc
     8701 cggccagggc catgtcggac tcgccggcga gcagcccctg gcaggccagg tggacggcca
     8761 ccagcgacgt ggagcaggcg ctctgcacgg tgaccgcggg cccgcccagg ctcagcttgt
     8821 aggcgatgcg ggtggcgaca tagtccttgt cgttggcgat ctgggcctgg aaatcgccga
     8881 tcgactggat gatccggggg ttggagtata ggttccagag gtagccgttg agcttggagc
     8941 cggcgtagac gccgaccaag ccggggaagc gctccgagtc gtagccggcc gactccatgg
     9001 cctcccaggc gcactccagg agaatacgct gctgcgggtc gatgatctcg gcctcgcgcg
     9061 ggctgcagtt gaagagctgg gcgtcgaaga ggtcctcccc ttcgatgagg cgcgccaccg
     9121 gcacgtagtc gggggagtcg atcgactccg gctcctccct cgactcctcc agctgctccc
     9181 gggtgaggac ggtgatggac tcgacgccgt cccggagatt ggcccagaac tcctccacgt
     9241 tgcgggcgcc gggaaaccgg caggccatgc cgacgatggc gatctccata ccggtcaggg
     9301 ggctctcgtt gctcatccgc ggtctctcct gtacgtccta gccttgttgc cgccgctgcc
     9361 gcagcttctg gaggcggctc tgccccgcct cgaccttgtc catgcgctcg tcgctctgcc
     9421 tcgccgccga caccaccgac accgtcgcga cctgccggcc ggcatgggcc gccagcaggg
     9481 ccaccgtcgg gtgggagaag agatcgacca cctgcaggtc gccgccgatc agctcactga
     9541 gccgggcgtg ggcccgcacc aacagcagcg agttgccccc ctgctcgaag aagttgtcgt
     9601 ggaggccgat ccggtcgagc cccagcacgt cctgaagg
//
LOCUS       DASPJI010000610        16271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_39012, whole genome shotgun sequence.
ACCESSION   DASPJI010000610 DASPJI010000000
VERSION     DASPJI010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16271)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16271
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39012"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VEL74_17000"
     CDS             <1..191
                     /locus_tag="VEL74_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64278.1"
                     /translation="HEVPLANGFLRTLDLALELRAGLPGTVLDTEQATYWQLLLQIPT
                     SGPDYETVFLAPVYSRRG"
     gene            351..1379
                     /locus_tag="VEL74_17005"
     CDS             351..1379
                     /locus_tag="VEL74_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64279.1"
                     /translation="MPLTPPPRRNRPKAAPGLPDGKKRIFAPPRSSVPVPGARVSGEG
                     RGPFWRLRASGGGGCFLSFFISIGLGLFLFGLAGTFGPYRDSLRESGDPRFFYAAMGM
                     GTLFVLMAARMLQLVLTDVEARARSQQKGKASEPWTWDYPWRPEEMDPDYTGAVGGAI
                     LGRVAFLAFIAFFNIAWMSGNWLFRAIIIVLDAFALLILVDALRAILQRGRFGRPKVE
                     WKTFPAFLGERLEGTVRFPRTLSPSGPAKATLRCVRDEWIQRPRPKGGTDSQLEAISI
                     HEEEHEIPLSDGRLERLDLAFELKDGLLGTRLDIEQAVYWQLLLQIPTLGPDYEVIFL
                     APVYSRRR"
     gene            1420..2103
                     /locus_tag="VEL74_17010"
     CDS             1420..2103
                     /locus_tag="VEL74_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006634088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HYG64280.1"
                     /translation="MRGGVTIQGRLVARPLAPISKRTAPGLHPLAFGGDRDGLLYVPA
                     GYRPDQPAPLALMLHGAGGDARGGLAPFQPLADNAGLLLLATDSRQQSWDVIRGGFGP
                     DVAFIDQALAWTFAHCAVDPGRVAAEGFSDGASYALSLAITNGELFTHVIAFSPGFVA
                     PAGYEGHPRIYISHGIGDRVLPIDSCSRKLVPRLKRSGYDVLYKEFDGPHTVPPEIAR
                     EALAWFLEK"
     gene            2107..3174
                     /locus_tag="VEL74_17015"
     CDS             2107..3174
                     /locus_tag="VEL74_17015"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HYG64281.1"
                     /translation="MKPDDDNGKRPLSERPFRVLILSGSDRRQYNCPGVDSKSRTLML
                     RMAERLPQEWEIDYEDLGNVFGRARIQSCNACVSTSMALCVWPCNCYEKKNKDEPDLM
                     WDLDLYARLDLADAWAVIGPVNWYAPTSNLKLMFDRLVCMNGGNPREELIDHKNPEKA
                     VRLEHSPEWDELSVNHLEGRTAGFFCYGDGGGDELDAAGRPKALRHPEWFDPDEEPFE
                     DLRQTYAPLVWQCRYSGIEVPDALWRYVEFGQGRKYSDNQAEHMPEQDVWAPLDAWAD
                     AFAAHVGRKGKVPPGKYRAYGYEPPKHRFADAKLGWRELRMRLGHPPQPSSPAAQQEL
                     GLNQDVTLRPSKGEGEKLREK"
     gene            3179..3910
                     /locus_tag="VEL74_17020"
     CDS             3179..3910
                     /locus_tag="VEL74_17020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HYG64282.1"
                     /translation="MSSLAIVVPVLNEAAVLEGFLADLLGREGDFEVILVDGGSDDGS
                     WEIAGRFREIRRLRSPRGRAAQMNAGARAAGADHLLFLHADTLLPPGAFERISEALAD
                     PAVAAGSFRLAFDRRDPWLAVYSFFSRINHPLFTYGDQGLFLRRELFREIGGFREIPI
                     MEDLEIQRRLRRRGRFVKLDLPVTTSARRFVHRGPLRQQALNVGLVSLYLLGMEPGRI
                     KRLYDDARPGARPRGASPSPSGGHR"
     gene            3907..4719
                     /locus_tag="VEL74_17025"
     CDS             3907..4719
                     /locus_tag="VEL74_17025"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HYG64283.1"
                     /translation="MKRKKVPVWISAPLVLGTFAALVWWERRRPLRPETEPKLRRNLR
                     NLAVAGVSAAVVQVAAAPVLKPLAEMVERRRWGLLRRLGLPVWLEVPLALVLLDYSLY
                     LWHVLAHRLPVLWRFHRVHHVDLDLDASTALRFHFGELTMSVAWNAGEILLLGITPLE
                     LSIRQTLLLTSILFHHSDVELPAGVERRLVRWIVTPRMHGIHHSIEEEETNSNWSSGL
                     TLWDRLHGTLRLDVPQEEITIGIPAYRDPEEVTLPRILSMPFGEQRPTWKLP"
     gene            complement(5509..6069)
                     /locus_tag="VEL74_17030"
     CDS             complement(5509..6069)
                     /locus_tag="VEL74_17030"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYG64284.1"
                     /translation="MLHFSRTALAGAALLALAAPVTAQTWQQPKEFQKPRDTWQQPGE
                     IQVPKGIQAIRAEDERCTKRFLVGADALFEFDKATLTPDAEETLEVLVPMLTKAGKHP
                     ATVEGHTDSKGADDYNQTLSEKRARTVKDWLVAHGALAATAPVQGWGERRPVAPNAKP
                     DGSDDPAGRQKNRRVAVILDLCAGKG"
     gene            7204..7848
                     /locus_tag="VEL74_17035"
     CDS             7204..7848
                     /locus_tag="VEL74_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HYG64285.1"
                     /translation="MSKLRVASFSISADGFGAGPNQSLDEPLGVGGESLHEWIVPTRT
                     FQRISGKESGTTGKDDEFTARGLENVGAWIMGRNMFGPVRGPWPDESWRGWWGQTPPY
                     HTQVFVLTHHPRPPLTMNGGTVFHFVTDGIHSALEQARHAAGGMDIRIGGGASTVRQY
                     LQAGLIDELHIAISPVLLGSGESLFSGLDMVALGYKCVEHVGTEKAMHIVLSKD"
     gene            7867..8274
                     /gene="tsaA"
                     /locus_tag="VEL74_17040"
     CDS             7867..8274
                     /gene="tsaA"
                     /locus_tag="VEL74_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018558694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HYG64286.1"
                     /translation="MFTIRPIGRVLSPLREREHAPKQGNEGAPEAWLEFEPEVQEGLA
                     DLRVGEEVLVLTWLHRADRETLRVHPRDDTSLPLRGVFSTRSQDRPNPVGIHRVRILE
                     IEGPTRLRVDGLEAFDGTPVIDLKPVLDREQER"
     gene            8335..8706
                     /locus_tag="VEL74_17045"
     CDS             8335..8706
                     /locus_tag="VEL74_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010363478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HYG64287.1"
                     /translation="MTHRLFVYGTLAPGRPNEHVLADVPGRWEPAAVNGTLLQEGWGA
                     AEGYPGIILDEHGGEVKGLLFSSERLAEHWARLDDFEGEGYERVLTAVKLEDGTAVGA
                     YIYRLSGNGSPAAGGGGPDVE"
     gene            8711..9142
                     /locus_tag="VEL74_17050"
     CDS             8711..9142
                     /locus_tag="VEL74_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64288.1"
                     /translation="MLLVKSLAVWLLILVLAIINGAFREAVLLPSLGRPLGLVLSGLL
                     LSVCIIVVACFFVPRMARTERLRPLYLGLLWLGLTLGFEFGFGRLVQRRSWPELLEAY
                     TFKDGNIWPIVLVVTFLAPLLAVWWAGKSREADGALVAKRD"
     gene            9155..9679
                     /locus_tag="VEL74_17055"
     CDS             9155..9679
                     /locus_tag="VEL74_17055"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014337706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HYG64289.1"
                     /translation="MTTALLIIDVQESLCVGAEAAFDIDRVVDRINSLSARARAVGAP
                     VILIQHEEEEGPLQFGSEGWQLYERLAIAPGDLRVRKTACDSFHQTGLEALLQARGVG
                     RLIICGLQSDYCIDATVRGAHALGYPVVLVSDAHSTMDSGGRTAAEITRHHNDALAGL
                     GSGVQPMPAAEVPL"
     gene            9676..10749
                     /locus_tag="VEL74_17060"
     CDS             9676..10749
                     /locus_tag="VEL74_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64290.1"
                     /translation="MTNTDPSQARRLVFLLVLGLTAAVFSGCAQVSSVAPVTEAKPAP
                     EGDCHRAESQRFVFHSDPWINLHHFLFEWARNVPERPPGDRGRAVDVTERTQLGSLEE
                     GDRRVWEQALGFYRERLVSRDLLFDRDLIVLRGQLASIACSGGNPDIIDNTELRTVLT
                     EAMPVYRKHWWPAHHAASMAWIRERLADLESYEATLSERLAQAYGGEWPADRIRADIT
                     AYAKWSGAYTTNQPDHVTISALGYQGLEGLEILFHEVSHASFFEQRLLGHLAAAFRQR
                     GAELPDRLSHVIQFATPPELLRSVLPGEKAGNDRSVAERVHERGSAIHLYRTVLEHWK
                     PFLDGKIERSEALDRIAAALALQ"
     gene            10872..12047
                     /locus_tag="VEL74_17065"
     CDS             10872..12047
                     /locus_tag="VEL74_17065"
                     /inference="COORDINATES: protein motif:HMM:NF017874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vps62-related protein"
                     /protein_id="HYG64291.1"
                     /translation="MKRLSLPSILCGLCVTAAFAVFGHPSPAAAQTCDGIGDGTWNGC
                     RGTGCQVCPEKVAGYDCYFINHPECVQNTSCGGLFYDCDAACPPPTEADICGNTNGDG
                     DSMSDALEDELLWRFAPVVRLHPNDSYRPSSADWYMARTHMRFHHTGCGDDQILNKGQ
                     ITTNNIDLQAHQNKNGWPGCSHNSTWQYSDMRTPNSRFFLQIPNDSAEGTTRLGAPSS
                     DWTCYAHVRYAPGSTSQYDIQYWFFYPYNGALMAGQGGHEGDWEHITVRVWNDGTSLS
                     QIYFASHDTEGQWFQPWQVSFTADDRPIVYSAVNSHASYPSPGTWVRNNLPDDHTADG
                     GPVWDCRRGVWNVGERGLPLNKGRYWLNYSGRWGEIGTAFSGPWGPAYNQNNYWNVN"
     gene            12118..13212
                     /locus_tag="VEL74_17070"
     CDS             12118..13212
                     /locus_tag="VEL74_17070"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HYG64292.1"
                     /translation="MAWKVRRTGAAEVPFRIKGKVGRIEDPPPAVFFRKYVEANRPVV
                     LSGVMEPWPALGRWNPAYLESVAGEHTGEVIVSKNGLYPDYISQPPPMAKVEMRLSEF
                     LRRAVPPENQRTAAGEPPPPILAPGETYYIYGKSYLLDAFPALREEIGAPACLGDVPV
                     STNSVWFSSPGCVTPLHYDLPNGLLCQVLGSKQVYLFDPSQHDLLYPRGRHFPGFDNF
                     ERQSQVDIQHPDFGAFPEFRRAEALECTLRQGDTLFIPSNWFHEVETLELSISVGFGF
                     AGGTSTSELAALSELFKKLSGGAPGAVNGMDLFRRAGEGPSGGPTPNLASDPRLMEEL
                     MSSPSVQRLLQDPAMMESVVRIMTGASQRS"
     gene            13212..13784
                     /locus_tag="VEL74_17075"
     CDS             13212..13784
                     /locus_tag="VEL74_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG64293.1"
                     /translation="MQAEVTTWHLEMLSPADLRPSGAEVPGLEIRQARIPSPELNRFL
                     YTAVGGDWFWIDRLCWTYEDWLRYLDRPEVETWVAYLEGTPAGYCELERQPGAAGDDV
                     EILYFGLLPRFIGRGLGGRLLTRAVERAWEMGASRVWVHTCSLDGPAALPAYRARGFR
                     VFKEVRSEQAVPAEPPGPWPGARDARMPRG"
     gene            13825..14568
                     /gene="map"
                     /locus_tag="VEL74_17080"
     CDS             13825..14568
                     /gene="map"
                     /locus_tag="VEL74_17080"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HYG64294.1"
                     /translation="MTIRTEQEMQSMQAVGRVVRAALEAMRRAVRPGISTAELDEVAH
                     GVLESHGARSAPKLVYNFPGHTCISVNDEIVHGIPGARVLQEGDLIKLDVTAEMNGFM
                     ADAACTVGVGKCSPEALRLVRCAQTAFRDAMKVAKAGARACDVGRVIERSARLQGFST
                     IRELCGHGIGKTIHEPPTMPNYFDPGCRDVLREGMVVAIEPMVSAGSRRSVMDRDGWT
                     VRTSDGSLAAHYEHTVVITKGKPIFLTAA"
     gene            14654..>16271
                     /locus_tag="VEL74_17085"
     CDS             14654..>16271
                     /locus_tag="VEL74_17085"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG64295.1"
                     /translation="MQLSSDGRNVVGQRQVSPIGRRADRGLALLSSGQRRLWFLDRWQ
                     PGSPAYSIASAFELTGRLHFAALAVALREIVRRHEILRTTFQEKGGEPVQVVTPVPAG
                     EHAAVAVVDLAALPVLVRRAEADRLATAQARRAFDLARGPLWRAVLLRIGPQTHTLLL
                     TLHHIVWDGWSTGILLRELEALYRSLSRGEAAILPELPIQYADFAVWQRRELSGGALN
                     GQLAYWRRQLTGAPALLELPTDHPRPPVQGFQGGTTGLFEIGPEASTGLSSLARPEGV
                     TQFILLFAAFAVLLHRYTASNDVVIGSPVAGRSRQETEGLIGFFVNMLALRVRPSGGQ
                     SFRELLGAVREVCLGAFDNQDLPFEALLEELQLERAPRHGPLFQVVLSLQNVSEARLG
                     LPGLSITTADVDTGAAKFDLMLALTPTSRGLAGSMTYRRELFESATAERLTRHLRTLL
                     GALTSAPDRAVGEVPIIGEDERRQILREWNPPAVERGAGDCLHRLFEETAGRAPDAVA
                     AVAGDLALTRAELHRRAGRLARRLRRLGVGP"
BASE COUNT         2532 a         5443 c         5548 g         2748 t
ORIGIN      
        1 atcacgaggt cccgctggcc aacggtttcc tgaggacgct cgacctcgcc ctcgagctgc
       61 gcgccggcct ccccgggacc gtcctcgata ccgagcaggc cacctactgg cagctcctcc
      121 tccagatccc cacctcggga ccggactacg agacggtctt cctggcgccg gtctattcgc
      181 ggcgtggcta ggatcgccgg ctagcggcct gtagggtcag cgccgagaaa ccggtcgccg
      241 cgaaaacgat gaaaagcgcc gggcgcaggg cggatcgcac ggccggcgag ttatacaggg
      301 tatgagaggg gcgggtcggg atagactagt cgcgttcgga ccggacccct atgcccctga
      361 caccgccccc gcgccggaat cggccgaagg ccgcgcccgg cctgcccgac ggcaagaaga
      421 ggatcttcgc gcccccccgg tcctcggtgc cggtccccgg cgcccgcgtc tccggcgagg
      481 ggagggggcc gttctggcgg ctgcgggcga gcggtggagg cggctgcttc ctgtcgttct
      541 tcatcagcat cggcctgggg ctcttcctct tcggcctggc cgggacgttc ggcccctatc
      601 gcgacagcct gcgcgagagc ggggatccca ggttcttcta cgcggccatg gggatgggta
      661 ccctgttcgt cctgatggcc gcgcggatgc tccagctcgt gctcaccgac gtggaggcgc
      721 gggcgcgctc ccagcagaaa ggcaaggctt cggagccgtg gacctgggat tatccctggc
      781 ggccggagga gatggacccc gactacaccg gcgcggtggg cggtgccatc ctcggccggg
      841 tcgccttcct ggccttcatc gccttcttca acatcgcctg gatgtccggg aactggctgt
      901 tccgggccat catcatcgtc ctcgacgcct tcgccctgct gatcctcgtc gacgcgttgc
      961 gggcgatcct gcagcggggc cgcttcggcc gccccaaggt ggagtggaag accttccccg
     1021 ccttcctcgg cgagcggctc gagggtaccg tccgcttccc ccgcacgctc tctccctcgg
     1081 ggccggcgaa ggcgacgctg cgctgtgtcc gggacgaatg gatccagcgg ccgaggccga
     1141 aagggggcac ggacagccaa ctcgaggcta tctcgatcca cgaggaggag cacgagatcc
     1201 cgctgtcgga tggacgcctc gagcgcctcg acctcgcctt cgagctgaag gacggcctcc
     1261 tcggcacccg gctcgacatc gagcaggccg tctactggca gctcctcctc cagatcccta
     1321 cccttgggcc tgactacgag gtcatcttcc tggcgccggt ctactcgcgg cgcagataag
     1381 acggacggct cgcaccgatc ttgcacctgg tgacaggtga tgagaggagg agtcacgatc
     1441 caggggcgcc tggtcgcccg tccactggcg ccgatctcga agcggaccgc tcccgggctg
     1501 caccccctgg cgttcggcgg cgaccgcgac ggtctcctct acgtgcccgc cggctaccgg
     1561 ccggaccagc ctgcccccct ggcgctcatg ctccacggcg cgggcggcga cgcacggggc
     1621 gggctggcgc cgttccagcc gctggccgac aacgccgggc tcctcctcct ggcgacggac
     1681 tcgcggcagc agagctggga cgtgatccgc ggcggcttcg gcccggacgt cgccttcatc
     1741 gaccaggcgc tggcctggac cttcgctcac tgcgcggtag accccggccg cgtcgccgcc
     1801 gagggcttct ccgacggcgc ctcctacgcg ctctccctcg ccatcacgaa cggtgagctc
     1861 ttcacccacg tcatcgcctt ctcgcccggg ttcgtggcgc cggccgggta cgaggggcat
     1921 ccgcggatct acatctcgca cgggatcgga gaccgggtgc tccccatcga ctcctgcagc
     1981 cggaagctcg tcccccggct gaagcggagc ggctacgacg tcctctacaa ggaattcgac
     2041 ggtccccaca cggtgccgcc ggagatcgcg cgcgaggcgc tggcctggtt cctggaaaag
     2101 tgagagatga aaccggatga cgacaacggc aaacgtcccc tttccgaacg ccccttccgt
     2161 gtcctgatcc tttcggggtc ggaccgccgg caatacaact gccccggcgt cgactccaag
     2221 agccggaccc tgatgctgcg catggccgag cgcctgccgc aggagtggga gatcgattac
     2281 gaagacctcg gcaacgtctt cggccgcgcc cgcatccaga gctgcaacgc ctgcgtctcc
     2341 acctccatgg ccctctgcgt ctggccttgc aactgttacg aaaagaaaaa caaggacgag
     2401 cccgacctga tgtgggacct cgacctctat gcccggctcg acctcgccga cgcctgggcc
     2461 gtgatcgggc cggtgaactg gtacgccccc accagcaacc tcaagctgat gttcgaccgt
     2521 ctggtctgca tgaacggcgg caatccgcgc gaggagctga tcgatcacaa gaaccccgag
     2581 aaggcggtgc ggctggagca ctcgccggag tgggacgagc tgagcgtcaa ccatctcgaa
     2641 ggccgtaccg ccggcttctt ctgttatggc gacggcggcg gcgacgagct cgacgccgcg
     2701 ggacggccga aggctctccg ccatcccgag tggttcgatc ccgatgagga gccgttcgag
     2761 gacctgcggc agacgtacgc gcccctggtc tggcagtgcc gctacagcgg catcgaggtg
     2821 ccggacgccc tctggcgcta cgtcgagttc ggccagggaa ggaagtacag cgacaaccag
     2881 gccgagcaca tgccggagca ggacgtctgg gcccccttgg acgcctgggc ggacgccttc
     2941 gcggcccatg tgggaaggaa agggaaggtg ccgcccggca agtaccgggc ctacggctac
     3001 gagccgccga agcaccggtt cgccgacgcc aagctcggct ggcgggagct gcgcatgcgg
     3061 ctcggccatc ctccccagcc ctcttcgccc gcggcgcagc aggagctcgg gctcaaccag
     3121 gacgtcaccc tgcgcccctc gaaaggggag ggggagaagc tgcgggagaa atagttcgat
     3181 gtcctccctc gccatcgtcg tcccggtcct gaacgaggcc gcggtcctgg agggcttcct
     3241 cgccgatctc ctgggccggg agggggactt cgaggtcatc ctggtcgatg gggggagcga
     3301 cgacgggagc tgggagatcg ccggccgctt ccgcgagatc cgccggctcc gctcgcctcg
     3361 gggccgggcg gcgcagatga acgccggcgc gcgggccgcc ggcgccgacc atctcctctt
     3421 cctgcacgcg gacaccctcc tgcctcccgg tgcgttcgag cggatttcgg aggcgctggc
     3481 ggacccggcc gtggccgcgg gctccttccg gctggccttc gaccgtcggg acccctggct
     3541 cgcggtctac tccttcttca gccggatcaa tcacccgctg ttcacctatg gcgaccaggg
     3601 gctgttcctg cggcgcgagc tgttccggga gatcggcggc ttccgggaga tccccatcat
     3661 ggaggatctg gagatccagc gccggctgcg gcggcggggc cgcttcgtca agctggacct
     3721 gccggtcacc acctcggcgc gccgcttcgt ccaccgcggg ccgctgcggc agcaggcgct
     3781 gaacgtcggc ctggtgtcgc tctacctgct ggggatggag ccgggacgga tcaagcggct
     3841 gtacgacgac gcccggccgg gagcccggcc gcggggagcg agcccgtctc cctcgggagg
     3901 gcatcgatga aaaggaagaa ggtcccggtc tggatcagcg cgcctctggt gctgggcacc
     3961 ttcgccgccc tcgtctggtg ggagcgccgc cggccgctcc gtccggagac cgagcccaag
     4021 ctgcggcgga acctgcgcaa cctggcggtg gccggcgtca gcgcggccgt cgtgcaggtg
     4081 gcagcggccc ctgtcctgaa gcccttggcg gagatggtgg agcggcgccg ctgggggctg
     4141 ctgcggcggc tgggcctgcc ggtctggctg gaggtgcctc tcgccctggt cctcctggat
     4201 tacagtcttt atctctggca cgtcctcgcg catcgcctgc cggtcctctg gcgtttccac
     4261 cgcgtccatc acgtggacct cgacctcgac gcctcgacgg ccctgcgctt ccacttcggg
     4321 gagctgacga tgtcggtggc ctggaacgcc ggagagatcc tgttgttggg catcaccccc
     4381 ctcgagctgt cgatccgcca gacgctgctg ctgacctcca tcctcttcca ccattcggac
     4441 gtcgagctgc ccgccggagt ggagcggcgg ctcgtgcgct ggatcgtgac gccccgcatg
     4501 catggcatcc atcactccat cgaggaagag gagaccaact ccaactggtc gagcggcctc
     4561 accctctggg accgcctcca cgggactcta cggctcgacg tgccgcagga agagatcacg
     4621 atcggcatcc ccgcgtatcg tgatcctgaa gaggtgacgc tgccccggat cctctccatg
     4681 cccttcggcg agcagcgccc cacctggaag ctgccctgag cgtacaaaac cgagcttctt
     4741 gatgtagggg cggggcttgt aaacgccccg aaggggccca ggcataaagc ctgccgtttc
     4801 aacggcaggt ggcgtcccca caaaccaccg aagccctgaa agggcgcagg cagctccttt
     4861 ctattgcgaa gcggtgcggc gccatcctcg acgagcgggt ggcggcgagc cccgagccgt
     4921 aagcacacgc tgcggcggag gcccctccgg gggcctccgc ctggccgcgc tcctcctgtc
     4981 ggacggagga gtcctcagga agcgcggcct tggacacggc ggcgcctccc tcacgattca
     5041 gttgtcaaag agccggggag ccggccgggt accgtttccg gaccggctcc tcagtcctac
     5101 gccgccatcc ccgcagcttc gggatgaatc cggacctttc gggacggctc catcacgact
     5161 cgccgccgct ccatgacgct cgggaagatt ccggagcgtt cgggagcgat cctcagcgat
     5221 ccgccgtctt cccacgcggc cgggattgat tccggagcgt tcgggatcga accgcgtcga
     5281 tccgccaccg tcctgaacgt cggggaagaa tcctcggtgc tgaggacaac gccgcggcca
     5341 gcataggagc caggccgcgc cgtgtcaata gagtcattga tggcgagata tggtatgatt
     5401 agctgactca tgaggcgcgg acggaggcat caatgtccga gtcgcccagc tctttgacaa
     5461 ctgacgtacc ttgggaccgc gggaggggcc cgcacccctc ccgcagacct agcccttgcc
     5521 cgcgcagagg tcgaggatga cggcgacgcg gcggttcttc tggcggccgg ccggatcgtc
     5581 cgagccgtcc ggcttcgcgt tcggcgccac cgggcggcgc tcgccccagc cctggacggg
     5641 ggccgtggcc gccagggcac cgtgggccac cagccagtcc ttgacggtcc gggcccgctt
     5701 ctcgctcaag gtctggttgt agtcgtccgc tcccttcgag tcggtgtgtc cctccaccgt
     5761 cgcggggtgc ttgccggcct tggtcagcat cggcaccagc acctccaggg tctcctcggc
     5821 gtccggggtc agcgtcgcct tgtcgaactc gaagagcgcg tcggcgccga ccaggaacct
     5881 cttggtgcag cgctcgtcct cggcgcggat cgcctggatc cccttgggca cctggatctc
     5941 cccgggctgc tgccacgtgt cgcggggctt ctgaaactcc ttcggctgct gccaggtctg
     6001 cgcggtcacc ggcgccgcga gagccagcag cgccgcgccg gcgagcgcgg tccgggaaaa
     6061 atggagcatg gtcgtcctcc ctggatgatt gagcagattc tagccgactc ggagcgcgtg
     6121 gaggtctctc gaccctcgcc aaacgccttt cgaccccagg gtcgagaggc tcgaccctgc
     6181 tcgaagcacg ctcgacccga ccaaaacctg gctcgaccct agggtcgagc cactcgaccc
     6241 gcttcgaaac gcacgcgacc ctagggtcga gaggactttg aagagggtcg agcctcctcc
     6301 gagccgggtc gaatgccgtc ggaagggcgt gacgtccctc cggcgggatg gttcgtcggc
     6361 ccggccggat ggtctatcga tccgaaggga tgtgaagccg atccggcggg atgaagcctc
     6421 ggtccggggg ggagtgtgtg ggccaggctc tcgccgatcc ggaacaggcc gaagccctct
     6481 ccggggatcg gctcgacccg ccaaggccct gcgacgtcgg cctcgcccgc cacgggcggg
     6541 tcaagcggct tggcgaagat tcttggagga ggttgggggg ggagggagga tgccgcctgg
     6601 tgcgccgggt tttcgacgtg acgtcgcatg atgtcagtag aagatggtag gctccgtcgg
     6661 cggatggcgc ccgcgctttc acgaggagcg ccgaagcttc agacgattga cagtgtaaat
     6721 gtccacggcg tacgttgagg aaaaggagaa atgatgaaca ggagcatcct tttgtgtggt
     6781 ctggtcgcgg ccctcgcgat ggtgttctcg attgctggtc cgccttctgc cgacgcttgg
     6841 ccttatattt atattgatga tcctaacctg gaatatccca gctgttgtgc cgacttcgag
     6901 cagttggaag gccagtcttg cccgacgggt gcgaggatct cctgtagctg gggggcctac
     6961 gggaggggat actgccagtg ctactacggc accggcgaga gttgggagtg ctactcgtcc
     7021 cagccgatcc ctgccaatcc ggatcagccg atctgctagc cgtgtgagcc ggggcgccgc
     7081 cggtgagcca tgagccggcg gtgcccctgc ggcatgagat gtacggggag gatgggagcc
     7141 ggcgaccgag gacggcacaa ccgtgaacgc cttcatttac agactcagta gacaggaggt
     7201 ccggtgagca aattgcgtgt tgcctcgttc tcgatctcgg cggatggttt cggcgctgga
     7261 ccgaatcaga gcctcgatga accgctcggc gtcggcgggg aatcgctgca cgagtggatt
     7321 gtgcccaccc gaaccttcca gagaatttcc ggtaaggaat ccggcacgac cggcaaggac
     7381 gacgagttta ccgcgagagg cctcgagaac gtcggcgcgt ggattatggg ccgcaacatg
     7441 ttcggccctg tccgcgggcc ttggcctgac gaatcctgga ggggctggtg gggacagacg
     7501 ccgccgtatc acacccaggt cttcgtcctg acgcatcacc cgaggccgcc cttgaccatg
     7561 aacggaggca ccgtcttcca ctttgtcacg gacggcatcc attcggcgtt ggagcaggcg
     7621 cggcacgcgg ccggagggat ggatatcagg atcggcgggg gcgcttccac cgttcgtcaa
     7681 tacttgcagg ccggcctcat agacgagttg catatcgcga tctcacccgt gctgctcggc
     7741 tccggagaga gcctgttctc ggggctcgac atggtcgcgc ttggttacaa gtgcgtggag
     7801 catgtgggaa ccgagaaggc catgcacatc gttttgtcga aggactagat ggcgctggac
     7861 tctccgatgt tcacgatccg acccattggc cgcgtcctgt cgccgctgcg ggaacgcgaa
     7921 cacgcgccga agcagggaaa cgagggggca cccgaggcgt ggctcgagtt cgagcccgag
     7981 gtccaggagg ggctcgccga cctgcgggta ggggaggagg ttctcgtcct tacctggctt
     8041 caccgggcgg accgcgagac cctgcgcgtg caccccagag atgacacgag cttgccgctg
     8101 cggggcgtct tcagtacccg ctcgcaggac cggccgaatc ccgtgggaat ccaccgggtg
     8161 aggattctcg agatcgaggg gcctacccgc ctccgggtcg acgggctgga ggctttcgac
     8221 gggacgccgg tcatcgacct caagcccgtg ctggaccggg agcaggagcg ttgacccgcc
     8281 caaccgccag actagactcg gcccgggttg agactcgctg cctctgggga atcgatgact
     8341 caccggctct tcgtttacgg aacgctcgcc ccgggacggc ccaacgagca cgtcctggcg
     8401 gatgttccgg gacggtggga gccggcggcc gtcaatggca cgttgctgca agaaggctgg
     8461 ggtgcggcgg agggctaccc gggaatcatc ctcgatgagc acggcggcga ggtcaagggt
     8521 cttctcttca gctcggagag attggctgag cactgggccc gtctcgatga tttcgagggt
     8581 gaaggctatg agcgcgtcct cacggcggtg aagctcgagg acgggacagc cgtgggcgcc
     8641 tatatctaca ggctcagcgg caatggctcg ccagccgccg gcggtggggg ccccgatgtc
     8701 gagtaggccg atgctcctgg tcaagtcgct cgctgtctgg ctgctcattc tcgtgctcgc
     8761 gatcatcaat ggcgccttcc gtgaggcggt gttgctcccc agcctgggca ggccgctcgg
     8821 gttggtgctg agtggcctgc tgctctcggt ttgcatcatt gtggttgcat gctttttcgt
     8881 accgcgcatg gcgcggacgg aaaggctcag gccgctgtac ctcgggctgc tctggctcgg
     8941 tctgacgctc ggattcgagt tcggcttcgg ccgcctggtg cagcgccgga gctggcccga
     9001 gttgctggag gcctacacat tcaaggacgg caacatctgg cctatcgttc tggttgtcac
     9061 gttcctcgcg ccgttgctgg cagtctggtg ggctgggaag agccgcgagg cagacggcgc
     9121 actagtggca aaacgagact gaaagaataa cgtcatgaca accgccttgc tcatcatcga
     9181 cgtacaggaa tctctatgcg tgggcgccga agcggcgttc gacatcgacc gcgtggtcga
     9241 cagaatcaat tcactctcgg ccagggcgcg agccgttggc gcgccggtga tcctcattca
     9301 gcacgaggaa gaggagggcc cgctccagtt cggcagcgaa ggatggcagc tttacgagcg
     9361 gctcgccatc gcgccgggcg atctccgggt caggaagaca gcctgtgatt cattccacca
     9421 aaccgggctc gaggcgctgc tccaggcgcg gggagtcggc aggttgatca tttgcggttt
     9481 gcagagcgac tactgcatcg acgcgacggt tcgcggagcg catgcgttgg ggtatccggt
     9541 ggtcctggtg tcggacgcgc attccaccat ggatagcggg ggacgcaccg ccgcggagat
     9601 caccaggcat cacaacgatg cgctggccgg gctcgggtcc ggtgtccagc cgatgcctgc
     9661 ggccgaggtg ccgctgtgac taataccgac ccctcgcaag cccgtaggct ggtcttcctg
     9721 ctcgtgctgg ggctcacggc cgccgtcttt tctggttgtg ctcaggtttc atcagtcgcg
     9781 ccggtcacgg aagcgaagcc ggcgccggag ggggactgcc atcgcgccga gtcgcagcgc
     9841 ttcgtcttcc acagcgatcc atggatcaat ctccaccatt tcctcttcga gtgggcgagg
     9901 aacgtgcccg agcggccgcc cggggaccgt ggccgagcgg tcgatgtaac ggagaggacg
     9961 cagctcggga gcctcgagga gggggatcgg cgggtgtggg agcaggctct gggcttctac
    10021 cgcgaacggc tggtctccag agacctcctg ttcgatcggg acctcatcgt cctcaggggc
    10081 cagttggcat cgatcgcttg ctcgggaggc aacccggaca ttatcgacaa caccgagctt
    10141 cggacggtgc tgaccgaagc catgccggtc taccggaagc attggtggcc ggcgcaccac
    10201 gccgccagta tggcttggat tcgggagcgg ctcgccgatc tagagtcgta cgaggcgact
    10261 ctctccgagc gcctggccca ggcctacggg ggcgaatggc cggcggatcg aatccgcgcc
    10321 gatatcacgg cctatgccaa gtggtcgggt gcgtacacga cgaaccagcc ggaccacgtg
    10381 accatctcgg ccctcggcta ccagggtctc gaaggcctgg agatcctctt ccacgaggta
    10441 tctcacgcga gcttcttcga gcagcggctc ctcggacacc tcgccgccgc gttccggcag
    10501 cgcggggccg agctgccgga tcggctctcc cacgtcatcc agttcgccac gccgccggag
    10561 cttctccgct ccgtgctgcc cggtgagaag gccggcaatg atcgctcggt tgcggagcgc
    10621 gtccacgagc gcggctcggc catccatcta tacaggaccg tgctcgaaca ctggaaacct
    10681 ttcctcgatg gaaagatcga gcgttctgaa gcgctcgacc ggatcgcggc ggcgctcgcg
    10741 ctacagtaag attcctgtgt agccgtggtt gaggtaacgg cgagcaccgc ccgcttcttg
    10801 ggaatggatc ttgcacttct gtggcttgat aatcaacgct cccctgagcg tgtcgaacca
    10861 ggaggtgcag tatgaaacgt ttatcccttc caagcatctt gtgcggcctg tgcgtcacgg
    10921 ccgcgtttgc cgtctttgga catccatcac cggccgcggc ccagacctgc gatgggatcg
    10981 gcgatgggac ttggaacggc tgccgcggca ccggctgcca ggtgtgcccg gagaaggtcg
    11041 ccggctatga ctgctacttc atcaatcatc cggaatgcgt ccagaatacg agctgcggcg
    11101 gcctgttcta tgactgcgac gcggcctgtc cgccgccgac cgaggcggac atctgcggca
    11161 acaccaacgg cgacggcgac agcatgtccg acgccctgga agacgagctt ctgtggcgct
    11221 tcgcgccggt cgtccggctg catccgaatg attcgtaccg gccgagcagc gcggactggt
    11281 atatggcacg gacccacatg aggttccatc acaccggctg tggcgacgat cagatcctca
    11341 acaagggaca gatcacgacc aacaacatcg acctgcaggc gcatcagaac aagaacggct
    11401 ggccgggctg cagccacaac agcacgtggc agtactcgga catgagaacc cccaacagcc
    11461 ggtttttcct gcagatcccg aacgactccg ctgaggggac cacccgcctg ggggctccgt
    11521 cgagcgactg gacctgctat gcgcacgtgc gctacgcgcc gggctcgaca agccagtacg
    11581 atatccaata ttggttcttc tatccttata acggcgcgct gatggcgggg cagggcggtc
    11641 acgaggggga ctgggagcac atcaccgtcc gcgtctggaa tgacgggacg tcgctctcgc
    11701 agatctactt cgcctcccat gataccgagg gtcagtggtt ccagccctgg caggtctcct
    11761 tcaccgccga tgatcggccc atcgtctatt ccgccgtgaa cagtcacgcc agctatccct
    11821 cgccggggac ctgggtccgc aacaacctgc cggacgacca taccgcggac ggcggtcccg
    11881 tctgggattg ccgtcgcggc gtctggaacg taggcgagcg cggattgccg ctcaacaaag
    11941 gccggtactg gctcaattac agcggccgtt ggggcgagat cggcacggcc ttcagcggtc
    12001 cttggggccc cgcctacaac cagaacaact actggaacgt caactaggcc gctgcacgga
    12061 gcccttgcct gcgccggccc ctcggcgcta aattctcggc ccacggggag gtgtccgatg
    12121 gcttggaaag tccgcaggac cggggctgcc gaggtcccgt tccggatcaa gggcaaagtt
    12181 ggcaggatcg aggacccgcc gccggcggtg ttcttccgga aatatgtcga ggcgaaccgg
    12241 ccggtggtgc tcagcggggt catggagccc tggccggcgc tggggcgctg gaatccggct
    12301 tacctggaat cggtggcggg ggagcatacg ggcgaggtca tcgtctcgaa gaacggcctc
    12361 tacccggact acatctcgca gcctcccccc atggccaagg tggagatgcg gctctccgag
    12421 ttcctgcgcc gcgccgtccc cccggagaac cagcggacgg cggcggggga gccgccgccg
    12481 ccgatcctcg ctccgggaga gacctactac atctatggca agtcgtacct cctggacgcc
    12541 ttcccggcct tgcgggagga gatcggcgcg ccggcctgcc tgggcgacgt cccggtgtcc
    12601 acgaacagcg tctggttcag ctcgcccgga tgcgtgacgc cgctgcacta tgacctgccc
    12661 aacggcctgc tctgccaggt gctcgggtcc aagcaggtct acctcttcga tccgtcgcag
    12721 cacgacctcc tgtatccgcg gggccggcat ttcccgggct tcgacaactt cgagcgccag
    12781 agccaggtgg acatccaaca cccggacttc ggcgccttcc cggagttccg gcgcgcggag
    12841 gcgttggagt gcacgctgcg gcagggggac acgctgttca tcccctccaa ctggttccac
    12901 gaggtggaga cgctggagct gtccatctcc gtcggcttcg gcttcgccgg cggcaccagc
    12961 accagcgagc tcgccgccct ttcggagctg ttcaagaagc tgtccggcgg cgcgccggga
    13021 gcggtcaacg gcatggacct gttccggcgg gccggcgagg gaccgtccgg cgggcccacc
    13081 cccaacctcg cctccgatcc ccggctgatg gaggagctga tgagctcccc ctcggtgcag
    13141 cgcctgctcc aggatccggc gatgatggag agcgtcgtcc ggatcatgac gggggcgagc
    13201 cagaggagtt gatgcaggcc gaggtcacca cctggcatct cgagatgctg tccccggccg
    13261 acctccggcc ctccggggcc gaggtccccg gcctggagat ccggcaggcg cggatcccgt
    13321 cgccggagct caaccgcttc ctctatacag ccgtcggcgg cgactggttc tggatcgacc
    13381 ggctctgctg gacctatgag gactggctcc gctacctcga tcggccggag gtggagacct
    13441 gggtggccta cctcgagggg acgccggccg ggtactgcga gctggagcgc cagccaggag
    13501 cggccggaga cgacgtggag atcctctact tcgggctgct ccccaggttc atcggccggg
    13561 gcctcggcgg ccggctgctg acccgggcgg tggagcgggc ctgggagatg ggggcgtcgc
    13621 gggtctgggt ccacacctgc tcgctggatg gccccgcggc cctgcccgcc tatcgggccc
    13681 ggggtttccg ggtcttcaag gaggtgaggt ccgagcaggc cgtgccggcg gagccgcccg
    13741 gcccctggcc gggcgcgcgg gatgctagga tgcctcgcgg atgagccccg gagagcccgg
    13801 gtcctccgag gagagcacga agacatgacg atccgtacgg aacaggagat gcagtcaatg
    13861 caggcggtgg ggcgcgtggt ccgcgcggcc ctggaggcga tgcggcgcgc cgtgcgcccg
    13921 gggatctcga ccgccgagct ggacgaggtg gcccatggtg tcctggagag ccacggggcc
    13981 cgctccgcgc ccaagctggt ctacaacttc cccggccaca cctgcatcag cgtcaacgac
    14041 gagatcgtcc acggcatccc cggggcgcgg gtgctccagg agggggacct gatcaagctc
    14101 gacgtgacgg cggagatgaa cggcttcatg gccgacgccg cctgcacggt gggggtgggg
    14161 aagtgctccc cggaggccct gcgcctcgtg cgctgcgccc agacggcgtt ccgcgacgcc
    14221 atgaaggtgg cgaaggcggg cgcccgcgcc tgcgacgtcg gccgggtgat cgagcgctcg
    14281 gcgcggctcc aggggttcag caccatccgc gagctgtgcg gccacggcat cggcaagacc
    14341 atccacgagc ccccgaccat gcccaactat ttcgacccgg ggtgccgcga cgtcctgcgg
    14401 gaggggatgg tggtcgccat cgagcccatg gtctccgccg gttcccggcg ctcggtcatg
    14461 gaccgggacg gctggacggt gcgcaccagc gacggcagcc tggccgccca ctacgagcac
    14521 acggtagtca tcaccaaggg caagcccatc ttcctgaccg cggcctgaga ccggggacct
    14581 ggagggatga gcatgatcca gggattgcgg accgcggggg cagtccacag aatctctatg
    14641 atatctggca tctatgcagc tttcttctga tggccggaac gttgttggcc agcgacaggt
    14701 ctctccgatc ggccgacgag cagatcgcgg gctcgcactg ctctcctcgg gccagcgccg
    14761 gctctggttc ctcgaccggt ggcaacccgg ttcccctgcc tacagcatcg cctcggcttt
    14821 cgagctgacg ggccgtctcc attttgccgc gctcgcggtg gccctgcggg agatcgtgcg
    14881 ccggcacgag atcctgcgca ccacgttcca ggagaagggg ggcgagccgg ttcaggtggt
    14941 gactccggtt cccgcggggg agcacgccgc cgtggcggtg gtcgaccttg cggccctgcc
    15001 tgtcctggtg cgccgcgccg aagcggaccg tttggcgacg gcgcaggcgc gccgcgcctt
    15061 cgatctggcg cgcggtccgc tctggcgcgc ggttctgctg cgcatcggcc ctcagacgca
    15121 taccctgctg ctcaccctgc accacatcgt gtgggatggc tggtcgaccg ggatcctgct
    15181 gcgggagctg gaggccctct accggagcct gtcgcgtggc gaggcggcga ttctgccaga
    15241 gctgccgatc cagtatgccg acttcgcggt ctggcagcga cgcgagctct ctggcggcgc
    15301 cctgaacggc cagctcgctt actggcggcg gcagctcacc ggggcgccgg cgctactcga
    15361 gctgcccacg gaccacccgc gtccgccggt ccaagggttc cagggcggga ccacagggct
    15421 cttcgagatc gggccggaag cgagcaccgg cctctcctct cttgcccggc cggaaggggt
    15481 aacgcagttc attctcctct tcgctgcctt cgccgtcctg ctgcaccgct ataccgcctc
    15541 aaatgacgtg gtgatcggct caccggtcgc cggccgcagc cgccaggaga cggaggggct
    15601 gatcggcttc ttcgtcaaca tgctggctct gcgggtacgg ccgtccggcg ggcagagctt
    15661 ccgcgagctg ctcggggctg tccgggaagt ctgcctgggg gctttcgaca accaggatct
    15721 gccgttcgaa gcgttgctcg aggagctgca actggagcgc gcaccgaggc acgggccgct
    15781 ctttcaggtg gtgctgtccc tccagaatgt cagcgaggcc cggctcgggc tgcccggcct
    15841 gagcatcacc accgcggacg tcgataccgg tgcggcgaag ttcgacctga tgctcgcgct
    15901 gaccccgacg tcacggggct tggccggcag catgacgtat cggcgggagc tcttcgaatc
    15961 cgccacggcc gaacggctca cccggcacct gcggaccctc ctcggcgcgc tgacgtccgc
    16021 tcccgatcgt gccgtgggcg aggtcccgat catcggtgag gacgaacgcc gccaaatcct
    16081 ccgggagtgg aatcctcccg cggtggagcg gggggccggg gactgtctcc accggctgtt
    16141 cgaggagacg gcgggccggg cgccggacgc ggtggccgcg gtggcggggg acctcgcact
    16201 gacccgcgcc gagctccacc gccgcgctgg ccggctcgcc cggcggctgc gccggctcgg
    16261 cgtcggcccc g
//
LOCUS       DASPJI010000611         3314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_209041, whole genome shotgun sequence.
ACCESSION   DASPJI010000611 DASPJI010000000
VERSION     DASPJI010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209041"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VEL74_17090"
     CDS             <1..814
                     /locus_tag="VEL74_17090"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HYG64296.1"
                     /translation="PVDLVPAEMHRLIEQLRTPAFEAAHPVLQASYAHYALVVIHPFA
                     DGNGRVARAVASTFFYRGQSIPLLIFSDRRPAYLDALQAADRGDPRPFIYFFRDRGIE
                     AVQLFTESLLETETPEPGSVVARLHRLLSRRQGLSREDMAALARNLLREIEREIEKQH
                     QELGGRGVRLGLTVRRTVDEQAFYAFLSAGGLSSGVGVQAWVQASTEESSPFSFRIKS
                     DHPGDFLEVRLEEVNPELSQSFRIRLSGWVQRLLGRTLLRLEQQIRDRTETP"
     gene            complement(856..1329)
                     /locus_tag="VEL74_17095"
     CDS             complement(856..1329)
                     /locus_tag="VEL74_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64297.1"
                     /translation="MSSPTNAFSAPFLARLGERDEPPTAGEAAVAGPWRIEPIPGEGF
                     GLFRAGESVARGHRPAAVCPSRWAAQVFAAMLPGTGRDPLLRLAKEPGGDGRYPVTLD
                     TGDLVARLEVFDDTLVEHVNALVHLLRIPDSLAHALEAGGAEALKRCGSILDERV"
     gene            complement(1877..>3314)
                     /locus_tag="VEL74_17100"
     CDS             complement(1877..>3314)
                     /locus_tag="VEL74_17100"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG64298.1"
                     /translation="LILLNHGAFTFDDDARVSYERMIDCVARAERAVAERRSRVAPSP
                     IYKEGGASSAWTPLDIGRLRAAVSRLAGRPMVLRLDSGPVARRFASHPGLPELARRGT
                     LTPDHVIRTKRIAAVVDDPGRIDEELERYGAEYRAYFEENVAGRPLEMLDPAPRVVVA
                     RGLGVFTAGATPADAQVVLDIYQHSMEAVLTAEELGGFRPLPAADLFEVEYWDLEQAK
                     LRRRPKPAPLAGRVAVVTGAASGIGRACAAALRAQGAAVAGLDLKPLADTGADLLGVP
                     CDVTDPAALRRALEETVHRFGGLDILVLNAGVFHSGPAIEELEDDAFRRVLELNTVSH
                     LSLLREAVPLLKLAPATPAISSVVIIGSKNVAAPGPGAAAYSASKAALTQIGRVAALE
                     LAPHGIRVNLLHPDGVFDTGVWTPEVLAQRAARYNLTVEQYKRRNLLGTEISSTDVGT
                     LAAALCTDLFAKTTGAQIPVDGGNDRVI"
BASE COUNT          517 a         1111 c         1165 g          521 t
ORIGIN      
        1 gccggtcgat ctcgtgccgg cggagatgca tcgcctcatc gagcagctcc ggacgcccgc
       61 cttcgaggcg gctcatcccg tgttgcaggc ctcgtacgcc cactatgccc tggtggtgat
      121 ccaccccttc gcggacggca acggccgggt ggcgcgggcg gtggcctcca ccttcttcta
      181 tagaggccag tcgatcccgc tcctcatctt ctcggaccgg cgacccgcct atctggacgc
      241 tctgcaggcg gcggatcgag gagacccaag gccgttcatc tacttcttcc gggatcgagg
      301 cattgaggcc gtgcagcttt tcacggaaag tctcctagaa accgaaacgc cggagcccgg
      361 gagcgttgtc gcccgactcc atcgtctcct ttcaaggcgg caggggcttt cgcgggagga
      421 tatggccgcc ttggcgcgga atctgctccg ggagatagag cgagagatcg aaaagcagca
      481 ccaggagctc ggcggtcgtg gggtgagact ggggctgaca gtcaggcgta ctgttgacga
      541 acaagcgttc tatgcctttt taagcgccgg ggggctctcc agcggagtag gcgtgcaggc
      601 ctgggtgcag gcctccacag aggagtctag cccgttcagc ttccggatca agagcgatca
      661 tcccggggac ttcctggagg tccgcctgga ggaggtgaat cccgagttgt cgcaaagctt
      721 ccggatcagg ctatcgggat gggtgcagag actcctgggg cgcaccctcc tgagattgga
      781 gcagcagatt cgggaccgaa cagaaacgcc gtgacccgct ccggacggcg gagcaggcca
      841 cggcgttttt tgcttctaca ctcgctcgtc gaggatggag ccgcaacgct tgagggcttc
      901 cgctccgccg gcctccaagg catgcgccag ggagtccggg atccgcagca gatggaccag
      961 ggcgttgacg tgctcgacca gggtgtcgtc gaagacctcc agccgcgcca cgagatcgcc
     1021 ggtgtccagc gtcacgggat agcggccgtc cccgccgggc tccttggcga gccggagcag
     1081 gggatcgcga cccgtgccgg ggagcatcgc ggcgaagacc tgggccgccc agcgcgacgg
     1141 acagaccgcc gccggccggt ggccgcgcgc cacgctctcg ccggcccgga agaggccgaa
     1201 gccctccccg ggaatcggct cgatccgcca tggaccggca accgccgcct cgccggccgt
     1261 gggcggctcg tccctctcgc ccagacgggc caggaacggt gcgctgaacg cgttggtggg
     1321 agaagacact acggacgagc tgctaccatc ggtgcaacca tgcataagga cctcgtatcc
     1381 ttgtgtcgtg gccaggcccg gtcgtgagtt tccacctcac ttctgggccg actcggcatc
     1441 agcgccgggc cacgtcctgc tgaactgcct gcaacagcga cttcaactgc gacgccggga
     1501 actgccggcg tgccctgcta ctacgtgatg acgagagtct agggaggttc ggcgcggaaa
     1561 tcaagcgtct tcgagaggag ctgtcctgca acgtcgcaaa gttcctctga atcgccttgc
     1621 gcaacggctc attcgtcgcg tagctcctga tgaacatcgc agagcagcgt tcgttcgtcg
     1681 cagatgagcc ttcgttcgtc gcagatcagt gttcattcgt cgcaaagctg cgtttattcg
     1741 tcgcagagct gcgttcattc gtcgcagagc gaagttgata tgcgacgaat gaacgctgat
     1801 ctgcgacgtt ctcctcgggt cgcaaccctc gggaactctg ctctgcgacg aacaatcctt
     1861 ccggacagga accccctcag atcacccggt cgttcccccc gtccaccggg atctgcgcgc
     1921 cggtggtctt ggcgaacagg tcggtgcaga gggcggcggc gagagttccg acgtcggtgg
     1981 aggagatctc ggtccccagg aggttgcggc gcttgtactg ctcgacggtg aggttgtagc
     2041 gggcggcgcg ctgggcgagg acctcggggg tccagacgcc ggtgtcgaag acgccgtcgg
     2101 ggtggaggag gttgacgcgg atgccgtgcg gggcgagctc cagcgccgcc acccttccga
     2161 tctgggtgag cgccgccttc gacgccgagt aggcggcggc tcccgggccc ggggcggcca
     2221 cgttcttcga gccgatgatc acgaccgagc tgatcgccgg ggtggccggc gccagcttga
     2281 gcagcggcac cgcctcgcgc agcagcgaca ggtgcgacac ggtgttgagc tccagcaccc
     2341 gccggaaggc gtcgtcctcc agttcctcga tcgccggccc ggagtggaag acgccggcgt
     2401 tcaggaccag gatgtcgagg ccgccgaagc ggtgcacggt ctcctccagc gcgcggcgca
     2461 gggcggccgg gtcggtgacg tcgcagggga cgcccaggag gtccgcgccg gtgtcggcga
     2521 ggggcttgag gtccaggccg gccaccgccg ccccctgggc gcgcagggcg gcggcgcagg
     2581 cgcggccgat ccccgaggcg gcgccggtga ccaccgccac ccggccggcc aggggggcgg
     2641 gcttcggccg gcggcggagc ttggcctgct ccaggtccca gtactccacc tcgaagaggt
     2701 cggcggcggg cagcggccgg aaccctccca gctcctcggc cgtgagcacc gcctccatgc
     2761 tgtgctggta gatgtccagc accacctggg cgtcggcggg cgtcgctccc gcggtgaaga
     2821 cgccgagccc ccgcgccacc accacccgcg gcgccgggtc gagcatctcc agcggccggc
     2881 cggcgacgtt ctcctcgaag taggcccggt actcggcgcc gtagcgctcc agctcctcgt
     2941 cgatccggcc gggatcgtcc accacggcgg cgatgcgctt ggtgcggatg acatggtccg
     3001 gcgtcagggt gccgcgccgc gccagctccg gcaggccggg gtgcgaggcg aagcggcggg
     3061 cgaccggtcc ggagtccagc cgcagcacca tgggccggcc ggcgagcctg gagacggcgg
     3121 cgcgcagccg gccgatgtcc agcggcgtcc aggcggacga agcgccccct tccttataga
     3181 taggggacgg cgccacccgg ctccgccgct cggcgaccgc ccgctccgcc cgcgccacgc
     3241 agtcgatcat ccgctcgtag gagacgcggg cgtcgtcgtc aaaggtgaag gcgccgtgat
     3301 tcaacaggat gagc
//
LOCUS       DASPJI010000612         3289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_552169, whole genome shotgun sequence.
ACCESSION   DASPJI010000612 DASPJI010000000
VERSION     DASPJI010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552169"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="VEL74_17105"
     CDS             complement(<1..1219)
                     /locus_tag="VEL74_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009181650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HYG64299.1"
                     /translation="MQRRRLWPYFAALGPGLLAASAGNDAGGIATYASAGASYGYGLL
                     WAMVIVTVCVGVVQEMSARMGAVTGKGFSDLVRENFSLRSTAVILLTLFVANFGIIVS
                     EFIGIAAAAELFGVSRYVAVPIAAVLVWLLITRGSYGWVEKIFLVLTLAFLAYAGAAF
                     LARPDWGAVLAGTVRPAPRFEPGYLQLLIALIGTTISPYMQLYVQSSVVEKGVTPEEY
                     RYTRFDVLMGTVFAGLIAAFIIIATAATLHPQGIEVETAADAAMALEPVAGSFARVLF
                     GLGLLGASLLAAGVLPLATTYMMSEALGFERGVSRTWEEAPIFMGLFTGLILLGAGLA
                     MIPGLPLIRVLVGVYVLNGLLLPVELFAMLRLIQNPEIMGPHVNRGAYNLLAWGIAIV
                     VSLLSLVLIVLTVA"
     gene            complement(1241..2476)
                     /locus_tag="VEL74_17110"
     CDS             complement(1241..2476)
                     /locus_tag="VEL74_17110"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYG64300.1"
                     /translation="MLFLTSIVDRPVQGKTGEPIGQLDDVIVRMGDERYPSLFGLLVK
                     DGRRRFFVPAAHLQALNGVARLSSSTVDLQPFSRRDGEVLLRRDVLDHQLIDVTGRRI
                     VRVNDVQLAQLEGVFRVVGIDISPQALLRRLAPRVLAGRIVGRQIIDWTEAQYLASAA
                     PVQLKVSYDRLAELNPVDLARIVDALSYREGAEIVAALDNETAAETLEEVSDERLATL
                     LEGMDQERAADILEEMTPGAAADALEDLEDEVAEQLLARMEPEEAADVQARLAYDEDS
                     VGRIMTTDFVRVPEGSTVGDAIAAVRGLEEVPDPLLAIYVVAADDPAELRGIVRLRNL
                     ILTEWNVPLAQVMDDDVPAVVPDDRAEDAARVLAEYNLLAVPVLEDGRLVGIVTVDDA
                     LAVLLPEVWQRRGVRAFAA"
BASE COUNT          538 a         1166 c         1157 g          428 t
ORIGIN      
        1 ccgcgacggt caggacgatg aggacgagcg acagcaggct gacgacgatg gcgatccccc
       61 acgccaggag gttgtaggcg ccccggttga cgtgcgggcc catgatctcg gggttctgga
      121 tcagccgcag catggcgaag agctccaccg gcagcagcag gccattgagc acgtagaccc
      181 ccaccagcac ccggatcagc ggcagcccgg ggatcatggc gagccccgcg ccgagcagga
      241 tcagcccggt gaagaggccc atgaagatgg gcgcctcctc ccaggtccgc gacaccccgc
      301 gctcgaaccc cagcgcctcg ctcatcatgt aggtggtggc gagcggcagc acccccgccg
      361 ccagcagcga ggcgccgagg agacccaggc cgaagagcac ccgggcgaag gagccggcga
      421 ccggctccag ggccatggcg gcgtcggcgg cggtctcgac ctcgatcccc tgggggtgga
      481 gcgtcgccgc cgtggcgatg atgatgaacg ccgcgatcag cccggcgaag accgtcccca
      541 tgaggacgtc gaagcgggtg tagcgatatt cctcgggggt cacccccttc tccaccaccg
      601 acgactggac gtagagctgc atgtacggcg agatggtggt gccgatgagc gcgatgagca
      661 gttgcaggta cccgggctcg aagcgcggcg ccggccgcac cgtcccggcg agcaccgcgc
      721 cccagtccgg ccgcgccagg aaggcggcgc cggcgtaggc caggaaggcg agcgtcagca
      781 ccaggaagat cttctccacc cagccgtacg agccgcgggt gatcagcagc cagaccagca
      841 cggcggcgat gggcacggcg acgtagcggc tgacgccgaa cagctcggcc gccgcggcga
      901 tgccgatgaa ctccgagacg atgatgccga agttggcgac gaacagcgtc agcaggatca
      961 ccgccgtgct gcgcagcgag aagttctccc gcaccaggtc ggagaacccc ttgccggtga
     1021 ccgcccccat gcgcgcgctc atctcctgga cgacgccgac gcagacggtg acgatgacca
     1081 tggcccagag caggccgtag ccgtaggacg cccccgccga cgcataggtg gcgatgccgc
     1141 cggcgtcgtt gccggcgctg gcggcgagga gcccggggcc gagggccgcg aagtagggcc
     1201 agagacggcg gcgctgcatc ccccctcccg gtctccctcg ctacgccgcg aaggcgcgca
     1261 ccccgcggcg ctgccagacc tcgggcagca gcaccgccag ggcgtcgtcc accgtcacga
     1321 tccccaccag ccggccgtcc tcgaggaccg gcaccgccag caggttgtac tcggcgagca
     1381 cccgcgccgc gtcctcggcg cggtcatccg gaacgacggc cggcacgtcg tcgtccatca
     1441 cctgggccag cggcacgttc cattccgtca ggatgaggtt gcgcagccgc acgatgccgc
     1501 gcagctccgc cgggtcgtcc gccgccacca cgtagatggc gagcagcggg tccggcacct
     1561 cctcgaggcc gcggacggcg gcgatggcat cccccaccgt gctcccctcg gggacgcgga
     1621 cgaagtcggt ggtcatgatc cggccgacgc tgtcctcgtc ataggcgagg cgcgcctgca
     1681 cgtcggcggc ctcctcgggc tccatgcggg ccaggagctg ctcggcgacc tcgtcctcca
     1741 ggtcctccag cgcgtcggcg gcggcgccgg gggtcatctc ctcgaggatg tcggcggcac
     1801 gctcctggtc catcccctcg agcagggtgg cgagccgctc gtcgctcacc tcctccagcg
     1861 tctcggcggc ggtctcgttg tcgagcgcgg cgacgatctc ggccccctcg cggtaggaga
     1921 gggcgtcgac gatgcgcgcc aggtccaccg ggttgagctc ggccagccgg tcgtaggaga
     1981 ccttgagctg gaccggcgcg gcgctcgcca ggtactgggc ctccgtccag tcgatgatct
     2041 ggcggccgac gatgcgtccc gcgaggacac ggggggcgag gcgccgaagc agcgcctgcg
     2101 ggctgatgtc gatcccgacg acccggaaga ccccttcgag ctgggcgagc tgcacgtcgt
     2161 tgacccgcac gatacggcgc ccggtgacgt cgatgagctg gtggtcgagc acgtcgcggc
     2221 gcagcagcac ctcgccgtcg cggcgggaga acggctgcag gtcgacggtg gaggaggaca
     2281 gccgggcaac gccgttgagc gcctgcaggt gggcggcggg cacgaagaac cggcgccggc
     2341 cgtccttgac cagcaggccg aagagcgacg gatagcgctc gtcgcccatg cggacgatga
     2401 cgtcgtcgag ctgcccgatg ggctcgccgg tcttcccctg cacggggcgg tcgacgatgg
     2461 aggtgaggaa cagcaccgcc tgcctccttt ccgatgccgc cgcaggcacc gggcccctct
     2521 cgcgagctat ccggaagcgt cagggggcgg ggaggcgggg cggtcaggcc gcgccttcag
     2581 gaggaaagcg cgtcagagac cggcgccgga gccctccgcg cgcggccgga ccgcgccagg
     2641 agtcgccgat gcatgaaagg gcaccaatgc gttggtgcaa ctagggccgt cgtccattac
     2701 cgtttcttga aggcgagcgc ccggaccaag ccggctcaga ctcgcgcgcc gaggatcgta
     2761 gcacgccgcc cgcagagggc acagccccac caacccgcaa actttggccc tcagggaaag
     2821 gtggtgcctc cccccctggg tggcgggagg gagggagcgg gggaggtgaa ggaggggggt
     2881 tgccttttct tctaatctcc tttcatgccg cttgacttac acaaagcgcc ctcatagaat
     2941 cccaccatgc cccacgacgc gatcctcatc gcgtcccctg gaaaggagga tctccggacc
     3001 tggaaggact tgaacccgcg gtacccgctg gagacgtcgt agccgtgcag tcgccgtacg
     3061 cagttcgaag cggggccggg tcgtccggcc cctgacctgc tgagcggccc gggagtgaga
     3121 gatcagctca cccccgggcc tacttcacaa gggagtttcg tcccacgcaa agcgatgacg
     3181 atcgtaacac cccccgtctg cccaacatct tcctccggga gttcctggac ctcctcggcc
     3241 gccgcgacga accgcccgcg gcggccgaag ccgccgtcgc cggtccctg
//
LOCUS       DASPJI010000613         2867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_39212, whole genome shotgun sequence.
ACCESSION   DASPJI010000613 DASPJI010000000
VERSION     DASPJI010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39212"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..736
                     /locus_tag="VEL74_17115"
     CDS             233..736
                     /locus_tag="VEL74_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYG64301.1"
                     /translation="MEIHDPAAFLDYYDKVRQRTDRVVACIPPERIEWTYREGKFTFG
                     DLLRHLAALERWMFAENAQGRPSRYPGHGRELADGYDAVMSYFHRLRQESRDLFAQLG
                     PEDLRKKCTTPAGAEITVWKWLRAMVEHEIHHRGQLYLYLSILGISGPPLYGLTSEEV
                     RERSADE"
     gene            729..2120
                     /locus_tag="VEL74_17120"
     CDS             729..2120
                     /locus_tag="VEL74_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64302.1"
                     /translation="MNEMEPPLTGAGPPGPERPERPDSPGRRTSEDVLGRTWEMELLI
                     SGGVLFALFQLSGALNQVFERAEPHLGLDSFVGLFLVYSLGKIILYTLIASFILHLAV
                     RGYWIGLIGLQSVFPAGPNWEQVKYGPLARQLYREMVPSLPRMIQRANGFCSVIFSFA
                     AILVLVCIVTIGWAGVFAVVAYGISKLFLDGQYLFPVLMALFMSASLVTLIAQGVDKA
                     LGERLVPAGRPARVIRAMLRGYYRLFLESLYRPIFLTLFSNFRKKAIYAVYYFVFFGL
                     FGLFIVKDLLIRRDVLTWSSYVYLPDSRDESAMDFVYYESQRPEGEIYRASPSIQDDV
                     IRDPYLELFIPYAPIRHDLAIRKRCPGVEPLAEPGPLFGLKESEPRPGSDPQAVLRCL
                     AGIHKVSLDGKPLRPDFLFYTHPRTEIRGILAYIPTEGLAKGSHLLRVEAVPKLRKED
                     APRPEPYLIRFWI"
     gene            complement(2096..>2867)
                     /locus_tag="VEL74_17125"
     CDS             complement(2096..>2867)
                     /locus_tag="VEL74_17125"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HYG64303.1"
                     /translation="LGIRLARAKSLGIFLVGFLLSVTPGKAGELGKAWMVRELGGGPA
                     LRVVPAVLAERVTDLLGVFLLLSLGALPFPGGRWIAGLGVAGVAAAVLLLTWKGMADL
                     LFRLLGRLPVVGKKVPVLVDLYATLRRLLSPDLLGIALFLSIVAWGAEGVGFFLVVRE
                     YAPRAELLAAVFNYTASTVLGSLSMLPGGLLAAEGSLTALLGAQGLDTAAAASATLII
                     RAATLWFAVLLGLAALPVVLRWLRRDPSPAPQIQKRIK"
BASE COUNT          449 a         1042 c          952 g          424 t
ORIGIN      
        1 tcctggagat cgtcggccgc cacgccccca acgggccggg cgccgtcctc ggcggcatgc
       61 acgtcgccca gcacctgctg gccggcggga ccctgcgcga ggacgaggcg cgcaccgccg
      121 ccgctgaagg ggcgatggag tccgtccgcc actgacgtat cccctgcccg cggcccggag
      181 cccctccggg ccgcgccggg cggccgtggt gggtctgcta ggctagagga tcatggaaat
      241 ccacgatccc gcagcctttc tggactacta cgacaaggtg cgccagcgga ccgaccgggt
      301 ggtggcgtgc atcccgccgg aacggatcga gtggacctac cgcgaaggga agttcacctt
      361 cggcgacctc ctccggcatc tcgccgccct ggaacgctgg atgttcgccg agaacgccca
      421 ggggaggccc agccggtatc cgggccacgg ccgcgagctc gccgacggct acgacgcggt
      481 gatgagctac ttccaccgcc tgcgccagga gtcccgcgac ctcttcgccc agctcggccc
      541 ggaggatctg cggaagaaat gcaccacccc cgcgggcgcc gagatcaccg tctggaagtg
      601 gctgcgcgcc atggtggagc acgagatcca ccaccgcggc cagctctacc tctatctctc
      661 gatcctgggg atttccggcc cgcctctcta tgggctcacc tcggaagagg tccgcgagag
      721 aagcgccgat gaatgagatg gagccaccgc tgaccggagc cgggcccccc ggccctgaac
      781 gtcccgaacg tcccgacagc cccggccgcc ggacctcgga ggacgtcctc ggccgcacct
      841 gggagatgga gctgctgatc tcgggcggcg tgctgttcgc gctgttccag ctctccggcg
      901 cgctgaacca ggtcttcgaa cgggccgagc cccacctggg cctcgactct ttcgtggggc
      961 tcttcctcgt ctactccctg ggcaagatca tcctctacac cctgatcgca tccttcatcc
     1021 tgcacctcgc ggtgcggggc tactggatcg ggctcatcgg cctgcagtcg gtgttcccgg
     1081 ccggccccaa ctgggagcag gtgaagtacg ggcccctcgc gcgccagctc tatcgggaga
     1141 tggtcccctc gctgccccgg atgatccagc gggcgaacgg cttctgcagc gtgatcttct
     1201 ccttcgccgc catcctggtc ctcgtctgca tcgtgacgat cggctgggcc ggggtcttcg
     1261 ccgtcgtggc ctacgggatc tcgaagctgt tcctggacgg ccagtacctg ttcccggtgc
     1321 tcatggcgct gttcatgtcg gcgtccctgg tcaccctgat cgcccagggc gtggacaagg
     1381 ctctgggcga gcggctggtc cccgccggcc ggcccgcgcg ggtgatccgg gcgatgctcc
     1441 gcggctatta caggttgttc ctcgaatccc tgtaccggcc gatcttcctt accctgttca
     1501 gcaacttccg gaagaaggcg atctacgccg tctattactt cgtcttcttc ggcctcttcg
     1561 ggctgttcat cgtcaaggac ctgctgatcc ggcgcgatgt cctgacctgg agctcctatg
     1621 tctatctccc ggactcgcgc gacgagagcg cgatggattt cgtctactac gagagccagc
     1681 ggcccgaggg ggagatctac cgcgccagcc cgtcgatcca ggacgacgtg atccgcgatc
     1741 cctacctcga gctcttcatc ccctacgcgc cgatccggca cgacctcgcg atcaggaaga
     1801 ggtgtcccgg cgtcgagccc ctggccgagc cgggaccgct attcggcctg aaggagtccg
     1861 agccgcgccc cggctccgat ccgcaggccg tgctgcgctg cctggccggg atccacaagg
     1921 tgtcgctcga cggcaagccg ctgcggccgg acttcctctt ctacacccac ccgcggacgg
     1981 agatccgggg catcctcgcc tacatcccca cggaggggct ggcgaagggg agccatctgt
     2041 tgagagtgga ggcggtgccg aaactgcgga aggaggacgc cccccggccc gagccctatt
     2101 tgatccgctt ctggatctga ggcgccggag agggatcgcg ccgcagccag cgcagcacga
     2161 ccggcaacgc cgcgagaccc aggagcaccg cgaaccagag ggtggcggcg cggatgatca
     2221 gggtggccga ggccgcggcg gcggtgtcga gcccctgggc acccaggagg gcggtgagcg
     2281 atccctcggc ggcgaggagc ccgccgggga gcatcgagag ggagccgagg accgtcgagg
     2341 ccgtgtagtt gaagacggcg gccagcagct ccgcccgcgg cgcgtactcc cgcaccacca
     2401 ggaagaaccc gaccccctcc gccccccagg cgacgatgga caggaagagg gcgatcccca
     2461 ggaggtccgg ggagagcagc cgccgcaagg tggcgtagag atcgaccagc accggcacct
     2521 tcttcccgac caccggcagc cgcccgagga gccggaagag gaggtcggcc atccccttcc
     2581 aggtgaggag gaggaccgcc gcggccacgc cggcgacgcc cagacccgcg atccagcgcc
     2641 cgcccgggaa ggggagcgcc ccgagcgaca ggaggaggaa gaccccgagc aggtccgtca
     2701 cccgctcggc cagcaccgcc ggcaccaccc gcagcgccgg tccgcccccc agctcgcgca
     2761 ccatccaggc cttgccgagc tccccggcct tgcccggcgt caccgagagg aggaagccga
     2821 ccaggaagat ccccaggctc ttcgcccggg cgagccggat gccgagc
//
LOCUS       DASPJI010000614         4642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_552313, whole genome shotgun sequence.
ACCESSION   DASPJI010000614 DASPJI010000000
VERSION     DASPJI010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552313"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4361)
                     /locus_tag="VEL74_17130"
     CDS             complement(<1..4361)
                     /locus_tag="VEL74_17130"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYG64304.1"
                     /translation="MSTLVDILRRRALEQPERLAYVFLADGEEEEARLTYAQLDERAR
                     AVAALLQELGIDRQDGGGRALLLFPTGPDYVAAFYGCLYAGVTAVPAYPPRPNRPMPR
                     IQAIAADARAGVALTTSAILSGVERRVADLPDLQALAWRATDVLDAGASSSWREPRIG
                     ADTLAFLQYTSGSTASPKGVMVSHANLLHNEALIRDACGHREDTPCVSWLPLYHDLGL
                     IGNMIQSLYVGTPCTLMAPVAFLQSPIRWLRAVSRYRAHTSGGPNFAYDLCARKITPE
                     QAEGLDLSSWRVAFNGAEPVRAETLERFARAFEPYGFRSEALFPCYGLAETTLIVSAG
                     AATAPPVVGRFRAGELARHRAVEAPEEVETRELVGCGQVLGDLEVAIVEPESLARRAE
                     GEVGEVWVAGGSVARGYWDRPEVTAETFEARTADGAGPFLRTGDLGFLRGGELYITGR
                     IKDLIIIRGANHYPQDIERTVELSHPALVPGASAAFSVDVAAEGGGEERLVVVQEIRR
                     DQRRADVDEIAAAARAALAAEHELQLHALVLIRPGTLPKTSSGKVQRRRTRQEFLEGT
                     LSVIGEWRESGRSGETAEAEPADLRSWLLARLGVAGGAGLSAPVAALGLDSLRAIELS
                     HEIEERTGTPFPLESFFGGLTVGQLVDEVLKAGAGIASSPTGLLPGSGSTDLSEGQRA
                     LWFLWRLEPASPAYNIAAAVRVRGAIDAAALHRAFQRLVERHPALRATFTAEGGEPVQ
                     RVQPDARLSFVWEDAAEWSEAELREHLAREAWRPFDLETGPLLRVTLLGWPAGESVLL
                     LAVHHIVSDMWSLAVLVRELGVLYAAATAPAGGTVPALPPLEVTYADWARWQRQMLNG
                     LDGERLRRYWEKKLAGELPALDMPTDRPRPPVQTDRGAAEAFRLSPALSEALRGLGKT
                     GRTTSFMTLLAVYQALLHRWTGQTDVLVGAPTSGRGRSQLANLVGYFVNPVVLRADLT
                     GEPTFAGFLEETQRTVLGAFEHADYPFPTLVERLAPVRDPSRSPVFQAMFALQNAPAV
                     DGQDLTAFAVGEEGAVLDVGGLRLEHLPLPQRIAQFDLTLSIGEVAGVFAATLDYNAD
                     LFDAATARRLAAGFETLLYAAADDPGRRIADLPLLPEPDRRQVLLDWNATAAPFRQDL
                     PVHVLFREQAAATPEEPAVVYGDLEVTYAELARRAAELAARLRALGAAPNTLIGLCTE
                     RSPELVVGLLGILETGAGYAPLDPEAPAERLVSLVDRAGISMVVTQDHLSFPWPPHLA
                     LLPLDREGGAVPGAPAGLAQATGSEEGEGGGAHTKEPSRAGLERLEQLRWAISPESPA
                     CLLYTSGSTGEPKGVLLTHRNLSNLIASFRASYTPGPGDAILPLTSVASASFVGEVLP
                     ALCSGAAVVLPDKGELLDFERTAGLIARRGISILSTVPSLVAGLNAIHDRVPKLRLLL
                     SGGEALSAGDVDRLLGTA"
     gene            complement(4358..>4642)
                     /locus_tag="VEL74_17135"
     CDS             complement(4358..>4642)
                     /locus_tag="VEL74_17135"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HYG64305.1"
                     /translation="AETVNSNAGGDPQRPFQVGFGVSQRFIADLADPGRSVAVNSTGQ
                     SGLPFHRHREDQAPMWARGEYHPVLTTREAARAQEESVLTLAPSQATGHR"
BASE COUNT          652 a         1716 c         1682 g          592 t
ORIGIN      
        1 gcggtgccga gcaggcggtc cacgtccccc gccgagagcg cctcgcctcc cgagaggagg
       61 aggcgcagct tggggaccct gtcgtggatg gcgttgaggc ccgccaccag cgacggcacg
      121 gtgctgagga tcgagatccc gcgccgcgcg atcaggccgg cggtgcgctc gaagtcgagg
      181 agctccccct tgtccgggag caccacggcg gcgcccgagc agagggcggg cagcacctcg
      241 cccacgaagc tcgccgaagc gaccgacgtc agcggcagga tcgcgtcgcc gggtcccggg
      301 gtgtagctgg cccggaacga ggcaatcagg ttcgacaggt tccggtgcgt cagcaggacg
      361 cccttgggct cgcccgtcga gccggaggtg tagagcaggc aggctgggct ctccggggag
      421 atcgcccacc tgagctgttc caggcgctcc agtccggccc tgctcggctc cttcgtgtgc
      481 gccccccctc cctcgccctc ttcgctgcca gtcgcctgcg ccaggccggc tggagcgcct
      541 ggaacagctc ccccctccct gtcgaggggg aggagagcga ggtgaggagg ccaggggaag
      601 gagagatggt cctgggtgac caccatggag atgccggcgc ggtcgacgag ggagacgagg
      661 cgctcggccg gagcctcggg gtcgaggggc gcgtagcccg ctcccgtttc caggatgccc
      721 aggaggccca ccaccaactc gggagagcgc tcggtgcaga ggccgatgag ggtgttcgga
      781 gccgctccca gggcgcgcag acgggcggcc agctcggcgg cgcggcgggc cagctcggcg
      841 taggtcacct ccaggtcgcc gtagaccacc gccggctcct ctggcgtcgc cgcggcctgc
      901 tcccggaaga ggacgtgcac cgggagatcc tggcggaagg gcgcggccgt ggcgttccag
      961 tcgagcaaga cctgacggcg gtccggctcg ggcagcagcg gcaggtcggc gatacggcgg
     1021 cccggatcgt cggccgcggc gtacagcagg gtctcgaagc cggcggcgag gcgccgcgcc
     1081 gtggcggcgt cgaagaggtc ggcgttgtag tccagggtgg cggcgaagac gcccgccacc
     1141 tcgccgatgg agagggtgag gtcgaactgg gcgatgcgct gcggcagcgg cagatgctcc
     1201 agccgcaggc cgcctacgtc gaggaccgct ccttcctccc ccaccgcgaa ggcggtcagg
     1261 tcctgtccgt ccacggcggg cgcgttctgc agcgcgaaca tggcctggaa gaccggcgac
     1321 cggctcggat cgcggaccgg cgccaggcgc tccacgagcg tcgggaaggg atagtcggcg
     1381 tgctcgaagg cgccgagcac ggtccgctgc gtctcctcca ggaagccggc gaaggtgggc
     1441 tcccccgtca ggtcggcgcg cagcaccacc gggttgacga agtagccgac caggttggcg
     1501 agctgggagc ggccgcggcc ggatgtcggc gcgccgacaa ggacgtcggt ctgcccggtc
     1561 cagcggtgga gcagcgcctg gtagaccgcc agcagcgtca tgaaggaggt ggtccggccg
     1621 gtcttcccca gcccacggag ggcttcggaa agcgccgggc tcagccggaa cgcctcggcc
     1681 gcgccgcggt cggtctgtac cggcgggcgc ggccggtccg tgggcatgtc gagcgccggc
     1741 agctcgccgg cgagcttctt ctcccagtac cggcgcagcc gctctccgtc gaggccgttg
     1801 agcatctgcc gctgccatcg tgcccagtcg gcgtaggtca cctccagggg aggcagggcc
     1861 gggacggtac cgcccgccgg cgccgtggcg gcggcataga ggacccccag ctcgcgcacc
     1921 agcacggcca gggaccacat gtcgctcacg atgtgatgga ccgccagcag gagcacgctc
     1981 tcccccgccg gccagccgag gagcgtcacc cgcagcagcg gcccggtctc gaggtcgaag
     2041 gggcgccagg cctcacgtgc cagatgctcg cgcagctccg cctcgctcca ctccgccgcg
     2101 tcctcccaga cgaaggagag ccgggcgtcg ggctgcacgc gctgcaccgg ctcgcccccc
     2161 tcggccgtga aggtggcgcg caacgccggg tggcgctcca ccagccgctg gaaggcgcgg
     2221 tgcagggccg cggcgtcgat cgccccacgc acccgcaccg ccgccgcgat gttgtacgcc
     2281 gggctcgccg gctccagccg ccagaggaac cagagcgccc gctgcccctc ggacagatcg
     2341 gtggaaccgc ttcccgggag cagtcctgtc ggggaggagg caatccccgc cccggccttc
     2401 aagacctcat cgaccagttg ccccaccgtc aacccgccga agaagctctc cagcgggaac
     2461 ggcgtgcccg tccgctcctc gatctcgtgc gacagctcga tggcgcgcag cgaatccagc
     2521 cccagggcgg ccaccggagc ggagaggccg gcgccgccgg ccacgccgag gcgcgccagc
     2581 agccagctcc gcagatcggc cggctcggcc tccgccgtct ccccggaccg gccgctctcc
     2641 cgccactccc cgatcacaga caaggtccct tcgaggaatt cctgccgggt gcggcggcgc
     2701 tgcaccttgc cgctggaggt cttgggcagc gtcccggggc ggatcagcac cagggcgtgg
     2761 agctgcagct cgtgctccgc ggccagggcg gcgcgggcgg cggcggcgat ctcgtccacg
     2821 tcggcccgcc gctggtcgcg ccggatctcc tgcaccacca ccagccgctc ctcgcctcct
     2881 ccctccgctg cgacatccac cgagaaggcg gccgaggcgc cgggcaccag cgcggggtgg
     2941 ctcagttcca ccgtgcgctc gatgtcctgc gggtagtggt tggcgccgcg gatgatgatc
     3001 aggtccttga tgcgaccggt gatgtacagc tcgccgccgc gcaggaagcc gaggtcgccg
     3061 gtgcgcagga acggcccggc accgtcggcc gtccgggctt cgaaggtctc cgccgtgacc
     3121 tcggggcggt cccagtagcc gcgcgccacg ctgccgcccg ccacccagac ctcccccacc
     3181 tcgccctcgg cgcggcgcgc cagcgactcc ggctcgacga tcgccacctc caggtcgccc
     3241 agcacctggc cgcaccccac cagctcccgc gtctccacct cttccggagc ctccaccgcc
     3301 cggtgccgcg ccagctcgcc ggcacggaag cggcccacca ccggcggcgc cgtggccgcg
     3361 ccggccgaga cgatcagcgt cgtctccgcc aagccgtagc aggggaacag ggcctcggac
     3421 cggaatccgt agggctcgaa ggcccgggcg aagcgctcca gggtctcggc ccgcaccggc
     3481 tccgcgccgt tgaaggccac ccgccagctc gacaggtcca acccctcggc ctgctccggc
     3541 gtgatcttgc gcgcgcagag gtcataggcg aagttcgggc cgccgctggt gtgggcgcgg
     3601 tagcgcgaga ccgcccgcag ccagcggatc gggctctgca ggaacgccac cggcgccatc
     3661 agcgtgcacg gcgtccccac atacagcgac tggatcatgt tcccgatcag ccccaggtcg
     3721 tggtagagcg gcagccagct cacgcagggg gtgtcctcac ggtggccgca ggcgtcccgg
     3781 atcagcgcct cgttgtgcag caggttggcg tggctgacca tcaccccttt ggggctggca
     3841 gtcgatcccg aggtgtactg gaggaaggcc agcgtgtcgg cgccgatccg cggctcacgc
     3901 cacgaggagg aggctccggc gtccaggacg tcggtggccc gccaggccag cgcctgcagg
     3961 tccggcaggt cggcgacgcg ccgctcgacg ccagagagga tcgccgacgt ggtcagcgcc
     4021 acacccgccc gggcatcggc ggcgatggcc tggatgcgcg gcatcggccg gttcggccgc
     4081 ggcggatagg ccggcacggc cgtcaccccg gcatagaggc agccgtagaa cgccgccacg
     4141 tagtccggac ccgtggggaa cagcagcagc gcccggcctc cgccgtcctg gcggtcgatg
     4201 cccagctcct gcagcagggc ggccacggca cgggcgcgct cgtccaactg ggcataggtg
     4261 agccgcgcct cctcttcttc gccgtcggcg aggaagacat aggccagacg ttcgggctgc
     4321 tcgagcgcgc gccggcggag gatgtcgacc agagtgctca tctgtgccct gttgcctgac
     4381 taggtgccag cgtcaatacc gactcctcct gcgcccgcgc cgcttcccgc gtggtgagca
     4441 ccgggtggta ctccccccgc gcccacatcg gcgcctggtc ctcgcggtgg cggtggaatg
     4501 gcagccccga ttgcccggtg ctgttgaccg ccaccgaccg cccggggtcc gcgaggtcgg
     4561 cgatgaaccg ctgcgagacg ccgaagccga cctggaacgg gcgctgcgga tcgccgccgg
     4621 cgttcgagtt caccgtctcg gc
//
LOCUS       DASPJI010000615         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_721081, whole genome shotgun sequence.
ACCESSION   DASPJI010000615 DASPJI010000000
VERSION     DASPJI010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721081"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VEL74_17140"
     CDS             <1..536
                     /locus_tag="VEL74_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64306.1"
                     /translation="PGFYYAVRVPASQITAAATAAGGDLAFHAMQFHTIINDSSLVPV
                     FAEATLATGTVSGNTAIIDLANPLARETNFVGQDNDFAPYYFKFPSVLGKKVLDGIAD
                     GSITDLFMVLRLPTAPLPFPGVSALPPFIGLDGGVAANDVPIYGLSYSSADGVTFTQS
                     TSFNFRFSLVLSQKPAA"
     gene            624..1706
                     /locus_tag="VEL74_17145"
     CDS             624..1706
                     /locus_tag="VEL74_17145"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HYG64307.1"
                     /translation="MQQAAGTTDLFLSLTPEKVLAAVEAGGLRTNPVCYPLNSFENRV
                     YEVELEDRTRVVAKFYRPGRWNEEQILEEHQFLADLAADELPVCTMRDFPGGGTLRQI
                     EDIWYALSDRRGGRAPDELDAATARRLGMLVGRMHNVAARRESSRRLRLTADAYARED
                     LFWLEDQDVVPRHLRQRYFAAAHAIADIADELMAGVPTHRIHGDLHLGNLLFRDGLLR
                     VLDFDDMMVGPAVQDIWLALPGRDARAVALRDALLEGYEQFRLFDRSTLRLVEPLRGL
                     RIIHYAVWLARRWHDPAFPAAWPHFGTPEYWDRETQDLEDQLAVIHGEAEDENGGGDG
                     GDGTGKSREEEPVLTNRDFFWDWEEN"
     gene            complement(1703..2803)
                     /locus_tag="VEL74_17150"
     CDS             complement(1703..2803)
                     /locus_tag="VEL74_17150"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HYG64308.1"
                     /translation="MAQEDRSIEIGGAPVVTLESPAPADADRPAFLSAQALPGRGMTT
                     LQIRAHLPSRGATDLLEAPPLEEARAFFDENPDEFQGNRSYLVGGAILLPYANRIRGR
                     LAPDGRTVETEVLGRTVHLPANSGGRRPGAERYSMHGLLLDARPDDLRHRTSDGEDRV
                     TAAYRPGDFGGRWPSATEVTIENVLRSDSFTLTVTARNTGGGPLPLGIGWHPYFALPA
                     GPRQQARMRIPARGRAIVNDYDEVLPTGEVEPVADTPYDFSMPGGRSLGDLYLDDCFV
                     DLERTAAGEVIAEVVDPAAAYGLRVVADSPFIRAIQVFAPPHRQIVVLEPQFNRADPF
                     GAQWGEGADTGMAILAPGESAFYSARLELFVP"
     gene            2859..>3503
                     /locus_tag="VEL74_17155"
     CDS             2859..>3503
                     /locus_tag="VEL74_17155"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HYG64309.1"
                     /translation="MRDLTFPDLRSFLDQLRRDADLMVVEAPVDARLEAAEIHRRVIA
                     AGGPALLFTNVRGADLPLTTNLFGTWRRAELAFGQRPLRLIRRLVHLAETLLPPTPAK
                     LWGARDLAREALRIGSSRRRSGPVSELVTSDVRLDRLPALTTWPEDGGPFITLPLVYT
                     EHPAGKGHNLGMYRLHVHDARSTGMHWQIGKGGGFHYGLAEAKGEALPVTVFLGG"
BASE COUNT          495 a         1296 c         1191 g          521 t
ORIGIN      
        1 accccggctt ctactacgcg gtgcgggtgc cggccagcca gatcacggcg gcggcgacgg
       61 cggcgggcgg agacctggcg ttccacgcca tgcagttcca caccatcatc aacgactcgt
      121 cgctcgtccc ggtgttcgcc gaggcgacgc tggcgacggg gacggtgagc ggcaacaccg
      181 ccatcatcga tctcgccaac ccgctggccc gcgagaccaa cttcgtcggc caggacaacg
      241 acttcgcccc gtactacttc aagttcccgt cggtcctggg caagaaggtc ctggacggca
      301 tcgccgacgg ctccatcacc gacctcttca tggtgctgcg cctgcccacg gcgccgctgc
      361 cgttcccggg cgtctccgcg ctgccgccgt tcatcggcct tgacggaggc gtggcagcca
      421 acgacgtgcc gatttacggc ctctcctaca gctcggcgga tggggtgacg ttcacccagt
      481 cgacgagctt caatttccgc ttctcgctgg tcctctccca gaaacccgca gcatagggct
      541 ccccgcccgg aaggcatccc aggagggagc ggctccggcc gctccctcct ttccgtcttc
      601 agcgtccgtg ctacattggc ctcatgcagc aagcagccgg caccaccgat ctgttcctgt
      661 ccctcacccc cgagaaggtg ctcgccgccg tcgaggcggg cgggctgcgg acgaatcccg
      721 tctgctatcc cctgaactcg ttcgagaacc gggtctacga ggtggagctc gaggaccgca
      781 cgcgggtggt ggccaagttc taccgtccgg ggcgttggaa cgaggagcag atcctggagg
      841 agcatcagtt cctcgccgac ctagccgccg acgagctgcc ggtctgcacc atgcgcgact
      901 tcccgggcgg cggcaccctg cggcagatcg aggacatctg gtacgccctc tcggaccgcc
      961 gcggcggccg cgcacccgac gagttggacg cggcgaccgc gcggcggctc ggcatgctgg
     1021 tggggcgcat gcacaacgtc gctgcccgcc gcgagtcctc ccggcggctg cgcctcaccg
     1081 ccgacgccta tgcccgggag gacctcttct ggctcgagga tcaggacgtg gtcccccgtc
     1141 acctgcggca gcgctacttc gccgccgccc acgccatcgc cgacatcgcc gacgaattga
     1201 tggccggggt gccgacgcac cgcatccacg gcgacctcca cctggggaac ctgctcttcc
     1261 gcgacggcct gctgcgggtc ctggacttcg acgacatgat ggtgggcccg gcggtgcagg
     1321 acatctggct ggccctcccc ggccgcgatg cacgggcggt cgccctgcgc gatgcgctcc
     1381 tcgaaggcta cgagcagttc cggctcttcg accgctcgac gctacgcctg gtggagccgc
     1441 tgcgcggcct gcgcatcatc cactatgccg tctggctggc ccgccgctgg cacgaccccg
     1501 cctttcccgc cgcctggccc cacttcggca cccccgagta ctgggaccgc gagacccagg
     1561 acctggagga ccagctcgcc gtcatccacg gcgaggccga ggacgagaat ggagggggcg
     1621 acggcggcga cgggaccggc aagtcccggg aggaggagcc cgtcctcacc aatcgggact
     1681 tcttctggga ctgggaagag aactagggga cgaacagctc cagccgcgcc gagtagaacg
     1741 ccgactcccc cggcgccagg atcgccattc cggtatcggc accctccccc cactgcgcgc
     1801 cgaacggatc ggcgcggttg aactgcggct ccagaacgac gatctgccgg tgcggcgggg
     1861 cgaagacctg gatcgcccgg atgaacggcg agtccgccac cacgcgcagc ccgtaggcgg
     1921 cggccgggtc caccacctcc gcgatcacct cccccgccgc cgtccgttcg aggtccacga
     1981 agcagtcgtc caggtagagg tcccccaggg aacggccccc cggcatggag aagtcatagg
     2041 gggtgtccgc tacgggctcc acctcgccgg tgggcagcac ctcgtcgtag tcgttgacga
     2101 tggcgcgccc gcgggccggg atgcgcatgc gcgcctgctg ccggggaccg gcgggcaggg
     2161 cgaagtaggg gtgccagccg atacccaggg ggagcggccc gccgccggtg ttcctggcgg
     2221 tgacggtgag ggtgaacgag tcgctccgca gcacgttctc gatcgtcacc tcggtggccg
     2281 acggccaccg gccgccgaag tcgccgggcc tgtaggccgc cgtgacgcgg tcctccccgt
     2341 ccgaggtccg gtgccgcagg tcatccgggc gcgcgtcgag gaggaggccg tgcatcgagt
     2401 accgttccgc gcccggccgc cggccccccg agttggccgg caggtggacc gtccgtccca
     2461 ggacctcggt ctcgactgtc cggccgtccg gcgccagccg gccgcggatg cggttggcgt
     2521 aggggagcag gatcgcgccg ccgacgagat acgagcggtt gccctggaac tcgtcggggt
     2581 tctcgtcgaa gaacgcccgg gcctcctcca gcgggggtgc ctcgaggagg tcggtggcgc
     2641 cgcgggaagg gaggtgggcc cggatctgca gggtcgtcat cccgcggccc ggcagcgcct
     2701 gggcgctcag gaaggcgggc cggtccgcgt ccgccggcgc cggactttcc agggtgacga
     2761 ccggcgcgcc gccgatctcg atagaacgat cttcctgtgc catgcgtcgc cctccttgct
     2821 agaccatggc cagcgtacta cgggatagac tctccgccgt gcgtgacctg acctttcccg
     2881 acctgcggtc ctttctcgac cagctccgcc gcgacgccga cctgatggtg gtggaggcgc
     2941 cggtagacgc ccgcctcgaa gcggccgaga tccaccgccg ggtgatcgcc gccggcggcc
     3001 cggccctgct cttcaccaat gtccggggag cggacctgcc gctgacgacg aacctcttcg
     3061 gcacctggcg ccgggccgag ctggccttcg gccagcgacc gttgcggctg atccggcggc
     3121 tggtccacct cgccgagacc ctcctgccgc cgaccccggc caagctctgg ggggcgcgcg
     3181 acctggcgcg ggaggcgctg cgcatcggct cttcccgcag gcgctccggc ccggtctccg
     3241 agctggtgac gtccgacgtc cgcctcgacc gcctgcccgc tttgaccact tggcccgagg
     3301 acggcggccc gttcatcacc ctccccctcg tctacaccga gcatccggcg ggcaagggcc
     3361 acaacctggg gatgtaccgg ctgcacgtcc acgatgcgcg gagcacgggc atgcactggc
     3421 agatcggcaa ggggggcggc tttcactacg gcctcgccga agccaaaggg gaggcgctgc
     3481 cggtcaccgt cttcctcggc ggc
//
LOCUS       DASPJI010000616         9669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_499405, whole genome shotgun sequence.
ACCESSION   DASPJI010000616 DASPJI010000000
VERSION     DASPJI010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9669)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9669
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499405"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VEL74_17160"
     CDS             complement(<1..233)
                     /locus_tag="VEL74_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64310.1"
                     /translation="MMDNPQYAAQQVVDLADLVTAPLIATVEADFLAVRRCLDLIREL
                     CFEPTPHQEGSPSDLGHLRTIRFRAEQPGPGGHP"
     gene            complement(223..603)
                     /locus_tag="VEL74_17165"
     CDS             complement(223..603)
                     /locus_tag="VEL74_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64311.1"
                     /translation="MPMLRDFIAAALQQAAEAQDLSNRFSRDLARDYQAGILQQLTVP
                     NAFLSELDLELRYAVAGSTPTREELRSTSGEKTPGPNVFDLEVIVAAEQLAQLPEAGL
                     GTLRLRIDFRDFGPRLIEERRYDG"
     gene            complement(699..1655)
                     /locus_tag="VEL74_17170"
     CDS             complement(699..1655)
                     /locus_tag="VEL74_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2589 domain-containing protein"
                     /protein_id="HYG64312.1"
                     /translation="MAIDTTPSTVATSALQAIPFGSLIGGPLQAAVQAQAMAAKTSWE
                     FIQQVGLSTDASGNKTAVSVQFQYQKGGQMVNLVVPLLTIVPIPYIAIDTIDINFVAN
                     ISASSSSVSEQSEDTQMGGSLGIKANVGWGPFSASADFNANYSSKKDSKATQESKYSV
                     EYTMNVAVHASQSAMPAGLAAVLNILQSSITEASPNGTLVVSPATGILDGSQKSPQQF
                     QLTLTSPSGLLVPNKQVQFSMPERFKDALQLKGSGDAKQDGASIVVTTDSNGLATVSV
                     ELAPNTSSLADVTTVRFEAKPVDNNSLKQFVTIQLFNIPAGS"
     gene            complement(2774..3229)
                     /locus_tag="VEL74_17175"
     CDS             complement(2774..3229)
                     /locus_tag="VEL74_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64313.1"
                     /translation="MTDFDELIPEMREWNNGNGIDVGAWIGCSGNFQLAVGYSTIFWP
                     RFVEFEGYVLRDGFSVDSLRGFQHRCAGDRKAVEAVMNHLHIADIQHYGCEDISRERI
                     VYLGQVLREIYEAKLAWQFPQRRFEVHLYAPDTDDLLDYEITFFQTEGT"
     gene            3294..4073
                     /locus_tag="VEL74_17180"
     CDS             3294..4073
                     /locus_tag="VEL74_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64314.1"
                     /translation="MSRSFKTALVVAHPGHELRVYSWVERERPEVFVLTDGSGSTGQS
                     RIPSTSEILSRTGARPGSVFGPFSDRGLYEVMLEGRIEILTALAHELADALAEGDVET
                     VAADAIEGFNPSHDICRVLTDAAVALARRRTGRAIGSFDFLLEGRPDACSAGSGSGVL
                     RLELEDAALERKLAAARSYPEMAHEVERAFESHGAGAFRIECLRPVDPEADIEALIEE
                     PPFYERHGERQVAAGRYSRVLRFREHFLPLARALRELGRTA"
     gene            4070..5440
                     /locus_tag="VEL74_17185"
     CDS             4070..5440
                     /locus_tag="VEL74_17185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG64315.1"
                     /translation="MSRPLRILFTNITLASRTGTEVYIKEAALGLLRRGHTPMVYSPN
                     LGEIAEEIRAATIPVVDDLSRLGAPPDIIHGHHHQQAMTALLHFPGVPGIFATHDWAA
                     WHDAPPRFPRIRRYVAVDWTNRDRILFEHGIPEERVRVILNWADLGRFPPRGPLPERP
                     RRALVFSNYARGDTHLPAVQEACRRAGLPLDVVGAGVGRSLADPGEVLAGYDLVFAKA
                     KSALEALAVGTAVVLCDYRGLGEMVTSTGFDALRRLNFGYRTLRQPLVPDLIVREIER
                     YDAGDAAEVSRRVREEAGLEAALDRLVALYEEVIGEHEREGPADPGEESRAAAAYLRD
                     WAGWTPHLRELAADIDQQRQRLDLRVRELEEELRAARLDRDGRLAVLEGELQAARRED
                     HGRLAELEEELRATRLDRDRLAEALGAVSGTITWRLRERLMSAPGLRAAYRTIRKLGG
                     ETGQRR"
     gene            complement(5428..6798)
                     /locus_tag="VEL74_17190"
     CDS             complement(5428..6798)
                     /locus_tag="VEL74_17190"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYG64316.1"
                     /translation="MTAKGVLRAAGWLSAVMAATCLGCRPAAERRPDVIVYLVDTLRA
                     DHLGCYGYPRETSPRIDAFAAESVLFENAVAQSAWTRPAVTSILTGLDPHSHGVQERL
                     DALPMSLDTLPEILRREGYQTAAFVSSAVITAKFGFDQGFDTFQQRVKETIEAERPTS
                     EWVNEEALRWLEQRDPDRPFFLFLHTLDPHMPYQPPEPFRRRLAPDADPRAGLLENVV
                     ALRDGQRPSSAREREEISALYDAEIAANDAAFGRLIAELRARGLYDPLVLVFVSDHGE
                     ELLDHGGWEHGASLYQELIHVPLMLKLPGRTGAGRRIGETVRQVDVLPTLLDVLGGKI
                     PPGIQGRSLLPLLQSPSRRPRRPPAAFSSLDVDGRRIESAILQDRKLIHTLAHDRRPA
                     GLELYDLAADPGERRNLAAADPATVRGLSWLLEQRARAGTPEKPPQVPLDPELEKQLR
                     ALGYLR"
     gene            complement(6897..7259)
                     /locus_tag="VEL74_17195"
     CDS             complement(6897..7259)
                     /locus_tag="VEL74_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HYG64317.1"
                     /translation="MPGFKEIVELTGLVVEGVGVLIIVLGLLFASARFALPGFRRAGS
                     AYRRYREDLGKAILLGLEFLVAGDIIRTVAVAPTLQNLLLLGLIVLIRTFLSMSLEVE
                     LEGRLPWKRNETAREAER"
     gene            complement(7261..7527)
                     /locus_tag="VEL74_17200"
     CDS             complement(7261..7527)
                     /locus_tag="VEL74_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64318.1"
                     /translation="MNPSTRWEGVTPRELRRSGVIWAVWFLVTSAYWLVLGNLMFRTN
                     LIPDDSAILLVTWIAAMILAIVSFFWTLARFVGSILLKQPPAES"
     gene            complement(7559..8509)
                     /locus_tag="VEL74_17205"
     CDS             complement(7559..8509)
                     /locus_tag="VEL74_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HYG64319.1"
                     /translation="MPVPRGPLPVVPPPRPRNLVEITIDGQSVAVPEGATLLEAARQI
                     GIDTPTLCYLENLTPVNVCRVCVVEVENARTLVPACSRKAEPGMVVKTDSERVRLSRR
                     MVLELLASSVDVSTAPQLQAYMARYGAQPERFGPPAPPAQAGERDAARPGHHHPPHDG
                     NAATVAQPVKVDNDLYVRDYSKCILCYKCVEACGTDAQNTFAIALAGRGFDARISTEY
                     DVPLPDSACVYCGNCIGVCPTGALMFKSEHDMREAGTWDESRQTATDTICAYCGVGCT
                     LTVHVQEEKIVKVTSPLDHDVTRGHLCIKGRFGWEFVNSR"
     gene            complement(8536..>9669)
                     /locus_tag="VEL74_17210"
     CDS             complement(8536..>9669)
                     /locus_tag="VEL74_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HYG64320.1"
                     /translation="RTVREVIDAKLVGRGGAAFPTGQKWLGARSAPLPRYLVCNADES
                     EPGTFKDRVIMEHDPFSLIEAMTIAGFATGCEKGYLYIRGEYPLATERLQNAIRRCYE
                     RGFLGANILGDGVRFDLELRRGAGAYICGEETALINSLEGRRGEPRNKPPFPTQVGLF
                     GQPTVINNVETLVNVLPILIDGGRAYAAIGTERSTGPKLFCVSGYVQRPGVYEFPFGI
                     TLRELIEAAGGVSGSGRLQAILLGGAAGGFVTPEQLDVPLTFEGTRAAGASLGSAVVM
                     PFDDTVDLRQALRRIAQFFRDESCGQCVPCRVGTVRQEELLHRLATDRPHGSLEQELG
                     LLRELGQAMRDASICGLGQTASSAVESALARFPIYLEKRQESR"
BASE COUNT         1549 a         3161 c         3344 g         1615 t
ORIGIN      
        1 ggatggccac cgggacccgg ttgctcggca cggaagcgga ttgtgcgcag gtggcccagg
       61 tcgctgggcg agccctcctg gtgaggagtc ggctcgaagc agagttcacg aatgaggtcg
      121 aggcacctgc ggacggcgag gaagtccgcc tcaacggtcg cgatcagagg agcggtgacg
      181 agatcggcga gatcgacgac ctgctgtgcg gcgtattgag gattatccat catagcgcct
      241 ttcttcgatc agccgtggac cgaagtcccg aaaatcgatt cgcaatcgaa gagtgcccag
      301 gccggcttcc ggaagctgcg cgagttgctc ggccgcaaca atcacttcca agtcgaagac
      361 gttcggacca ggcgtctttt cgccgctcgt gcttctcagc tcctctcttg ttggagtgga
      421 gcccgcaacg gcgtagcgca actcgaggtc caactccgag aggaatgcgt ttgggacggt
      481 aagttgctgc agaatacccg cttgatagtc tcgggcaagg tcccgggaga accggttgga
      541 aaggtcctgg gcctctgcgg cctgctgcag cgctgcggca atgaaatctc tcagcatcgg
      601 catgtgaact gcctccgttg gtcagaagaa ggccgtcccg gccggactgc ggtcgggacg
      661 gaaggataca ccccgagcga agctgtagga tcttctaatc agcttcccgc tggaatgttg
      721 aacaactgga tcgtcacgaa ctgcttgagt gagttgttgt caactggctt ggcttcgaag
      781 cggaccgtgg tgacgtccgc gagcgaggag gtgttcgggg ccagttccac ggagaccgtc
      841 gccaagccgt tcgaatcggt ggtgaccacg atagatgcgc catcctgctt cgcatcgcca
      901 ctgcccttca actggagtgc gtccttaaat ctttcaggca tagagaactg cacctgtttg
      961 ttgggcacga ggaggccgct cggactggtg agggtgagtt ggaattgctg tggcgacttc
     1021 tggctgccgt ccagaatgcc ggtggctggg ctcacgacaa gagtcccatt cgggctggcc
     1081 tcggtgatcg aggactgaag gatgttgagg acggcggcga gcccggctgg catcgccgac
     1141 tgcgaggcat ggaccgccac gttcatcgtg tactcgaccg agtacttcga ttcctgcgtg
     1201 gctttcgagt cctttttgct cgagtagttg gcgttgaagt cggcgctggc gctgaagggc
     1261 ccccagccca cgttcgcctt gatcccgagc gagccgccca tctgcgtgtc ctcggactgt
     1321 tccgagaccg acgacgagga cgccgagatg tttgcgacga agttgatgtc gatcgtgtcg
     1381 atcgcgatgt aggggatcgg cacgatggtc aggaggggaa cgactagatt caccatctga
     1441 ccccccttct gatactggaa ctgaaccgag acggcggtct tgttcccgct ggcatcggtg
     1501 cttaggccga cctgctggat gaattcccaa ctcgtcttgg ctgccatcgc ctgcgcctgg
     1561 acggcggcct ggagaggacc tccgatcagt gagccgaacg ggattgcctg aagcgcgctg
     1621 gtcgcgaccg tcgaaggcgt ggtgtcgatt gccattttct tctccttgat tgtcgatcgg
     1681 tgtcgacgga taccggccgg cggcgatctg cctcacggtt cggaaattcc ggggccccac
     1741 cgtgggttgt cagcgacagc cttcgtcaga acagctttca cttgtatccg tcagacctaa
     1801 gacggttccg gcattcgtat tgtgaatctc catctggtgg ggctgggcta gcctctcgcg
     1861 ttcgcgtgtg ggcgtgtcgt ttctgtccag ccatgccgag atcttcgcct ttaagatgca
     1921 gtcacgactt cacaatcggc aggagacgac tgtgccgcga gtggctcgtg gggccgagct
     1981 cttccggtgg cagatcgaag gagtctcact tgcacgctat cggtagtacc tgtacatctc
     2041 ctgcggagcc ggggaccacc tgtctctcga actacctttg cacgatctgg ctgcagcata
     2101 gggctgaaat gctctatctg tggcggaggt gcacacgtca ggaagatgat gcggagaagg
     2161 ccttcagccg cgcgagcctc ctcctctatc gcaagctgcc cgcccattgg gagcgggtgg
     2221 aaaaacaacg tagctggatt ctgcgtttga cgttcaacgt ctgcatgagc ctgcaccggg
     2281 agaaccgcca gcgggccgag aagagcctcg aagaggcggg tcgctggcct ggctgctgga
     2341 ggcggcgggc ggggtcgctc tcgaacgctg cggcgccatc ctcgacgagc ggacgggaca
     2401 tccttataga tgggcgtcac cggcaggctc ggcgtgccgg tgacgctctc tttttcctcc
     2461 tctcgccccg cgggtcgaga ggtctgcccc agggggcaag cggttcgccc cttggggtaa
     2521 gccttcttca tcccgggtca agcggtttga cccacggggc gagccatccg acccgtgggt
     2581 caggtggttc gcccctcggg gcaagtcatc ggccccacgg ggcaggaggt tcgaccccag
     2641 gagcgagtgc attccatccc ggggcaagcc gccgtgatct tggggcaagc cctctgcccc
     2701 gcgggtcaag tggtctgccc ctcacaattg aagggccccg ccccgggcgc gctcgatctc
     2761 cagcctctgc ggctcaggtg ccttcggtct ggaagaaggt gatctcgtag tcgaggagat
     2821 cgtcggtgtc cggggcgtag aggtgcactt cgaagcggcg ctgggggaac tgccacgcca
     2881 gcttcgcttc gtaaatctcc cgcagcacct gcccgaggta gacgatgcgc tcgcggctga
     2941 tgtcctcgca gccgtaatgc tggatgtcgg cgatatgaag gtgattcatc actgcctcga
     3001 ccgccttgcg gtctccagcg catcggtgct ggaagccccg cagggaatcc accgagaagc
     3061 catcgcgcag gacataacct tcgaactcga caaatcgggg ccagaagatc gtgctgtagc
     3121 cgaccgccaa ctggaagttg cccgagcagc cgatccacgc cccgacgtcg atgccgttgc
     3181 cgttgttcca ctcccgcatc tcggggataa gctcatcgaa gtcggtcatg tgctcaatct
     3241 tcccacagtc ccgggcgtag aggagaggca caggtctcca gtaaactccc cggatgtcga
     3301 gatcgttcaa gactgccctc gtggtcgctc atcccggcca cgagcttcgc gtttatagct
     3361 gggtcgaacg ggagcggccg gaggtcttcg tgttgacgga cggctcgggg agcaccggcc
     3421 agtcccgcat tccctccacc tcggagatcc tgtcccgcac cggtgcccgg ccgggctccg
     3481 tgttcggccc gttcagcgac cgcggcctgt acgaggtgat gctggagggg cggatcgaga
     3541 tcctcacggc tctggcgcac gagctcgccg acgccttggc cgaaggggac gtggagacgg
     3601 tcgctgccga cgccatcgag gggttcaacc cctcccacga catctgccgc gtgttgaccg
     3661 acgcggccgt cgcgctggcc cgccgccgga ccgggcgggc gatcggcagc ttcgatttcc
     3721 tcctcgaagg acgcccggac gcgtgctccg cgggcagcgg ctccggggtc ctgcggctgg
     3781 agctggagga cgccgcgctg gagcgcaagc tggcggcggc gcggtcctat cccgagatgg
     3841 cgcacgaggt cgagcgggcg ttcgagagcc acggcgccgg cgccttccgt atcgagtgcc
     3901 tgcggccggt cgatcccgag gcggacatcg aggcgctgat cgaggagccg ccgttctacg
     3961 agaggcacgg cgagcggcag gtggcggccg gccgctattc tcgggtcctg cgcttccgcg
     4021 agcacttcct gccgctggcg agggccttgc gcgagctcgg gcgcaccgca tgagccggcc
     4081 cttgcgcatc ctgttcacga acatcaccct ggcatcccgc accgggacgg aggtctacat
     4141 caaggaggcc gccctggggc tcctgcggcg ggggcacacc ccgatggtct acagccccaa
     4201 cctcggcgag atcgcggagg agatccgcgc cgccacgatt cccgtggtcg acgacctctc
     4261 ccggctcgga gcccctccgg acatcatcca cgggcaccac caccagcagg ccatgaccgc
     4321 cctgctgcac ttccccgggg tgcccggaat cttcgccacg cacgattggg cggcctggca
     4381 cgatgccccg ccgcgcttcc cgcgcatccg ccgctacgtc gcggtcgact ggacgaaccg
     4441 cgaccgcatc ctgttcgagc acggcatccc ggaagagcgc gtccgggtca tcctgaactg
     4501 ggccgatctc ggaaggttcc cgccgcgcgg cccccttccc gagcggccgc ggcgggctct
     4561 ggtgttcagc aactacgccc ggggggacac ccacctcccg gcggtccagg aggcctgccg
     4621 gcgcgccggt ctccccctgg acgtggtggg cgcgggcgtc ggaaggtccc tggccgaccc
     4681 gggcgaggtg ctcgccgggt acgacctcgt cttcgccaag gccaagagcg ccctggaggc
     4741 gttggcggtg gggacggcgg tggtgctctg cgactatcgc ggcctcggcg agatggtcac
     4801 cagcaccggc ttcgacgccc tgcgccggct gaacttcggc tatcgcaccc tgcgccagcc
     4861 gctcgtcccg gacctcatcg tgcgcgagat cgagcgctac gacgcgggcg acgccgccga
     4921 ggtctcgcgc cgggtgcggg aagaggcggg cctcgaagcg gccctcgacc gcctcgtcgc
     4981 cctctacgag gaggtgatcg gcgagcacga gcgcgagggg ccggccgatc ccggcgagga
     5041 gtcgcgggcc gccgcggcct acctgcgcga ctgggcgggc tggacgccgc acctccggga
     5101 gctggccgcg gacattgacc agcagaggca gcggctcgat ctccgagtga gggagctgga
     5161 ggaggagctc cgggccgccc ggctggaccg cgacggccgc ctggcggtgc tggagggaga
     5221 gctccaggcc gctcggcggg aggaccacgg ccgtctggcc gagttggaag aggagctccg
     5281 ggccacccgc ttggaccgcg accgcctggc ggaggcgctc ggcgccgtct ccgggacgat
     5341 cacctggcgc ctgcgcgaga ggctgatgag cgcccctggc ctgcgggcgg cctatcgaac
     5401 gatccgcaag ctcggagggg agacgggtca gcgcaggtaa ccgagcgccc ggagctgctt
     5461 ctccagctcg ggatccaggg gcacctgggg gggcttttcc ggcgttccgg cccgcgccct
     5521 ctgctcgagg agccacgaca ggccgcggac cgtggccggg tcggccgccg cgaggttgcg
     5581 ccgctctccg ggatccgccg cgaggtcgta cagctccagg ccggccggcc ttcggtcgtg
     5641 ggccagcgtg tggatcagct tccggtcttg caggatggcg ctctcgatcc gccggccgtc
     5701 gacatcgagg gacgagaacg ccgccggcgg ccggcgcggc cggcgcgagg gggactggag
     5761 caggggcagg aggctcctgc cctggatgcc tggcgggatc ttgccgccca ggacatcgag
     5821 gagcgtcggc aggacgtcga cctgccggac cgtctctccg atcctccgcc cggcgccggt
     5881 cctccccggc agcttcagca tcaaggggac atggatcagc tcctgataga gagaggcacc
     5941 atgctcccac cccccgtgat ccaggagctc ctcgccgtga tcggagacga agaccagcac
     6001 gagagggtcg tagagcccgc gtgcccgcag ctccgcgatc agccggccga acgcggcgtc
     6061 gttggccgcg atctcggcat cgtagagcgc cgagatctcc tcccgctcgc gcgccgagga
     6121 gggccgctgg ccgtcgcgta gcgcgacgac gttctcgagc aggccggcgc gaggatcggc
     6181 gtccggagcc agccgccggc ggaagggctc cggcggctgg tagggcatgt gggggtccag
     6241 ggtgtggagg aagaggaaga acgggcggtc cggatcccgc tgctccagcc accgcagcgc
     6301 ctcctcgttc acccattccg aggtcgggcg ctccgcctcg atcgtctcct tgacccgctg
     6361 ctggaaggtg tcgaacccct gatcgaaccc gaatttcgcg gtgatcaccg cgctggagac
     6421 gaaggccgcc gtctggtaac cctcccggcg caggatctcg ggcagcgtgt cgagcgacat
     6481 cggcagtgcg tcgaggcgtt cctggactcc gtggctgtgc gggtcgagac cggtcaggat
     6541 cgaggtgacg gcaggccggg tccacgccga ctgcgccacg gcgttttcga acaggacgct
     6601 ctccgcggcg aaggcatcga tccggggcga cgtttcgcga ggatacccat agcagccgag
     6661 gtgatcggcg cgcagcgtgt cgaccagata gacgatgacg tcgggccgtc gttccgccgc
     6721 gggacggcag ccgaggcacg tcgcggccat cacggcactg agccagccag cggctctgag
     6781 gactcccttt gcggtcatcg aagcgtctcc ggctcagcgc ttctcaccat gtcaatcgag
     6841 gcagagctcg gtcaggcgag cgagccgtgg atcttcccgg gacacatgcc ggcattctag
     6901 cgctctgcct cacgagccgt ttcgttgcgc ttccagggca ggcgcccttc gagctcgacc
     6961 tccagggaca tgctgaggaa ggtccggatc agcacgatca ggccgaggag gaggaggttc
     7021 tgcagggtcg gagccacggc gaccgtacgg atgatgtcgc cggcgaccag gaactccaga
     7081 ccgaggagga tggcctttcc cagatcctcc cggtaccgcc ggtaggcgct gcccgctctc
     7141 cggaagccgg ggagggcgaa ccgcgccgac gcgaacagca gcccgaggac gatgatcagg
     7201 acgcccacgc cctccaccac caggccggtc agctcgacga tctctttgaa gcccggcacg
     7261 tcagctctct gcgggcggct gtttcaacag gatcgatccc acgaaccgcg ccagggtcca
     7321 gaaaaaggag acgatggcca ggatcatcgc cgcgatccag gtcaccagga ggattgcgct
     7381 gtcgtccgga atgagattgg ttcggaacat caggtttccc agcaccagcc agtaggccga
     7441 ggtgacgagg aaccagaccg cccagatgac gccactccgg cggagctcgc gaggcgtgac
     7501 gccctcccag cgggtgctcg ggttcaacca ctcacctaag gacatggcaa ccccgtttct
     7561 aacgcgagtt cacgaattcc cacccgaagc gccccttgat gcagaggtgg ccgcgggtga
     7621 cgtcgtgatc gagcggggag gtgactttca cgatcttctc ttcctgcacg tggacggtga
     7681 gcgtgcagcc cacgccgcag taggcgcaga tcgtatcggt cgccgtctgg cgcgactcgt
     7741 cccaggtgcc ggcctcgcgc atgtcgtgct cgctcttgaa catcaacgcg ccggtggggc
     7801 agacgccgat gcagttgccg cagtagacgc aggcggagtc gggcagcggg acgtcgtact
     7861 cggtcgagat gcgggcgtcg aagccgcgcc cggccagggc gatggcgaag gtgttctggg
     7921 cgtccgtgcc gcaggcctcg acgcacttgt agcagaggat gcacttcgag tagtcgcgca
     7981 cgtagagatc attgtccact ttgaccggct gggccacggt ggcggcgttg ccgtcatggg
     8041 gcgggtgatg atgaccggga cgggcggcgt cgcgctcgcc ggcctgggcg ggcggagccg
     8101 gcgggccgaa gcgctccggc tgcgcgccat agcgcgccat gtaggcctgg agctgcggcg
     8161 ccgtcgagac gtccaccgac gaggcgagga gctccagcac catgcgccgc gagaggcgca
     8221 cgcgctcgga gtcggtcttc accaccatgc ccggctccgc cttgcgcgag caggccggca
     8281 ccaacgtgcg ggcgttctcc acctcgacca cgcagacgcg gcagacgttg accggcgtca
     8341 ggttttccag ataacagagg gtcggcgtgt cgataccgat ttgccgcgcc gcttcgagga
     8401 gcgtcgctcc ctcgggcacg gccacgctct ggccgtcgat ggtgatctcc accaggttgc
     8461 gggggcgcgg cggcggcacc accggcagcg ggccgcgggg caccggcatt ttcagatcgg
     8521 gagcgttctg catggtcatc tcgattcctg acgcttttcc agatagatgg ggaaccgggc
     8581 gagggccgac tcgaccgcgc tcgacgcggt ctggcccagg ccgcagatgg atgcgtcgcg
     8641 catcgcctgg ccgagctcgc ggagcagtcc cagctcctgc tcgagggagc cgtgcggccg
     8701 gtccgtcgcc agccggtgca gcagctcctc ctggcgcacg gtgccgaccc ggcaggggac
     8761 gcactggccg cacgattcat cgcggaaaaa ctgagcgatc cgccgtagcg cctgccgcag
     8821 gtcgacggtg tcgtcgaacg gcatgaccac cgccgagccg aggctggcgc cggcggcccg
     8881 cgtcccttca aaagtgagcg gtacgtcgag ctgctcgggg gtgacgaagc caccggccgc
     8941 gccgccgagg aggatcgcct gcagccggcc gctgccgctc acgccgccgg ccgcttcgat
     9001 cagctcgcgc agggtgatgc cgaaggggaa ctcgtagacg ccggggcgct gcacgtagcc
     9061 ggagacgcag aacagcttgg ggccggtcga gcgctccgtg ccgatggccg cgtaggccct
     9121 gccgccgtcg atcaatatcg gcaggacgtt gaccagcgtc tcgacgttgt tgatcaccgt
     9181 cggctggcca aagagaccca cctgcgtcgg gaacggcggc ttgttgcgcg gctcgccgcg
     9241 gcgcccttcg agcgaattga tcagcgcagt ctcctcgccg cagatgtacg ccccggcgcc
     9301 gcgccgcagc tcgaggtcga accgtacgcc gtcccccagg atgttggcgc cgaggaagcc
     9361 gcgctcgtag cagcgccgga tggcgttctg gagcctttcg gtcgcgaggg gatattcgcc
     9421 gcggatgtac agataccctt tttcacagcc cgtggcgaag ccggcgatgg tcatcgcttc
     9481 gatcaaggag aacggatcgt gctccatgat cacgcggtcc ttgaacgtcc ccggctccga
     9541 ctcgtcggcg ttgcagacca ggtagcgcgg cagcggcgcc gagcgggcac cgagccactt
     9601 ctggccggtg gggaaggcgg cgccgcctct gccgacgagc ttcgcgtcga tcacctcgcg
     9661 caccgtgcg
//
LOCUS       DASPJI010000617         3505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_438338, whole genome shotgun sequence.
ACCESSION   DASPJI010000617 DASPJI010000000
VERSION     DASPJI010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438338"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..929)
                     /locus_tag="VEL74_17215"
     CDS             complement(105..929)
                     /locus_tag="VEL74_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYG64321.1"
                     /translation="MVEANARSAGPQVSPLLLSMFGRYCEGYLGRHFHAVRLSRAQRP
                     DPAMARGKPLVLYFNHPSWWDPLICLQMARQLFPDRRHYGPIDARALGKYRFFAKLGF
                     FPVETGTSRGARQFLTASQQVLGQPDAALWIPAAGRFSDPRERPLRLQPAMGHLASRL
                     RQGVFVPVALEYPFWEERFPEALVRFGEEVSVADAGLSGNDWGVVLEERLTAAQEALA
                     AESITRDPGRFEALLGGSAGVGGVYDVWRRFRARLRGEKFSAAHGDEPPGRDEEDL"
     gene            complement(1392..2963)
                     /gene="crtI"
                     /locus_tag="VEL74_17220"
     CDS             complement(1392..2963)
                     /gene="crtI"
                     /locus_tag="VEL74_17220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017929881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HYG64322.1"
                     /translation="MTETTDRIGVLGAGLGGLAAAVTLAARGHRVVLFERNPWLGGKA
                     AVLHEQGFRFDMGPTILTLPSVLGRIFGEAGRDLRDELELVPLDPQWRCFYDDGSALD
                     LVADRRRMAEILAAFAPGSGADAGYGRFLDMAERLHGISDRYFFWRSVGSVRDTFDAG
                     TTFKASVLSDLLSLRFGRTVAGTVRRHVPEPRVAQMLDHFTQYVGSAPDASPAVLCGI
                     AHMQTAEGIWYPMGGTRAVPEALERLAASLGVEVRRGTGVRRILTEETGRRRGRRRVT
                     GVVTEQGERVPLAGVVSNADSVRTHRELLSEEDERAIHRFERRRAYEPACSGVVLYLG
                     LDRRYPQLLHHNFVFSHDPEEEFDAIYRRGEPAADPTCYVCAPAATEPAVAPAGGEAL
                     YILVHTPYLRPHHDWRRMLPAYRRTILDKLASTAGLADLEKHIVCEAALTPEDIHHRY
                     SVLDGAIYGLASHGRMLGAFKPANRSPDVDGLYLAGGAAHPGPGMPMVLMSGWIAADA
                     LDRDAGAEVSRRRSA"
     gene            complement(2960..>3505)
                     /locus_tag="VEL74_17225"
     CDS             complement(2960..>3505)
                     /locus_tag="VEL74_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003606606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HYG64323.1"
                     /translation="YVQNASVTDLGLAPPGMSTLYVLVPVTHQHPNVDWRQERDRYRA
                     VALRQLEKLGIEDVERRIRYERVVTPLDWDQEFEIHRGATFNLAHNLGQMLHLRPRNR
                     FEDLEGVYLVGGGTHPGSGLPVIFESALITARLLLQDFGGAAAAHPFHRSGAPDRSPA
                     APPLAGVGNSGIGKAAREAGL"
BASE COUNT          503 a         1236 c         1307 g          459 t
ORIGIN      
        1 ctccgaaagg gggggccgcc ggtagagctg gaggttccgc cagaaggtca gggcggggag
       61 gagggcgcag agcgcggcga caatggccag gatggagagg gtggtcagag atcctcctcg
      121 tcgcgcccgg ggggctcgtc gccgtgggcg gcggagaact tctcgccgcg cagccgcgcc
      181 cggaagcggc gccagacgtc atagacgccg ccgaccccgg cgctgccgcc gaggagcgcc
      241 tcgaagcggc ccgggtcgcg ggtgatggac tccgccgcca gcgcctcctg ggcggcggtc
      301 aggcgctcct ccaggaccac cccccagtcg ttgccggaga ggccggcgtc cgccaccgag
      361 acctcctcgc cgaagcgcac cagcgcctcc gggaagcgct cctcccagaa cgggtattcc
      421 agcgctacgg gcacgaagac cccctggcgc aggcggctgg cgaggtgccc catggcgggc
      481 tgcagccgca gcgggcgctc ccggggatcg ctgaagcggc cggcggcagg gatccagagc
      541 gccgcgtcgg gctgtcccag cacctgctgc gaggcggtga ggaactgccg tgcgccgcgg
      601 gaggtgccgg tctccacggg gaagaagccg agcttggcga agaaccggta cttgccgagc
      661 gcccgcgcgt cgatcgggcc gtagtggcgg cggtcaggga agagctgccg cgccatctgc
      721 aggcagatca gggggtccca ccaggagggg tgattgaagt agaggaccag cggcttgccc
      781 cgcgccatgg ccgggtccgg gcgctgcgcg cgcgacagcc gcacggcgtg gaaatggcgg
      841 ccgaggtagc cctcgcaata gcggccgaac atcgagagca ggagcggcga cacctggggg
      901 ccggcgctcc gggcgtttgc ctccaccacg gtcagagtct aaaccgggag agagatggat
      961 gcacggccct cacccctcgg tccccctctc ccgcctcccc aacccctccc cgggagaggg
     1021 ggaggccgga acggggtttt cgccgcccgt ccacccggac gtggggtctg tccctcgttc
     1081 cccgaaagaa aaggaagcga gccccgtcgc cgtggatgcg gaaacggagc tcgtccctcc
     1141 cggggcagcc gttgcccccg gaggaatggc cttcataccc ggagagatgg cttccgtacc
     1201 ccgggtacgg tgtccgtacc cgcgggtatg gtttccgtac cccgggtagg gaagccctcc
     1261 cctcggagag gatctccgta ccccgggtag gaaggccgta cccgcgggta gggtcttcct
     1321 acccggggta gcgtcgtcgt ctcgggggag atggtggccg tttcaccggg tatggaggtt
     1381 gtttcgaggg cctacgccga cctccgccgg ctcacctcgg ctccggcgtc ccggtcgagg
     1441 gcgtcggcgg cgatccagcc ggacatcagg accatgggca tgcccggccc ggggtgcgcc
     1501 gcgccgcccg cgaggtagag cccgtccacg tcggggctgc ggttggccgg cttgaaggcg
     1561 ccgagcatgc ggccgtggct cgccaggccg tagatcgccc cgtcgaggac ggagtagcga
     1621 tggtggatgt cctccggcgt cagcgccgcc tcgcagacga tgtgcttctc cagatccgcg
     1681 agaccggccg tggaggccag cttgtccagg atggtccgcc ggtaggcggg gagcatccgc
     1741 cgccagtcgt ggtgcgggcg caggtaggga gtgtggacga ggatgtagag cgcttcgccc
     1801 cccgccggcg ccaccgccgg ctcggtggcc gccggcgcgc agacgtagca ggtggggtcc
     1861 gccgccggct cgccacgccg gtagatggcg tcgaactcct cctcggggtc gtgggagaag
     1921 acgaagttgt gatgcaggag ctgcgggtag cgccggtcga ggcccaggta gagcaccacc
     1981 cccgagcagg ccggctcgta ggcgcggcgg cgctcgaagc ggtggatggc ccgctcgtcc
     2041 tcctccgaca acagctcgcg gtgggtgcgt accgagtcgg cgttcgagac gacgccggcc
     2101 agcggcaccc gctccccctg ctcggtgacc acgcccgtca ccctgcggcg tccccggcgg
     2161 cggccggtct cctcggtcag gatgcgccgc acccccgtgc cccgccggac ctcgacgcca
     2221 aggctggccg ccagccgctc cagcgcctcg gggacggcgc gggtgccccc catggggtac
     2281 cagatcccct ccgccgtctg catgtgggcg atgccgcaga gcaccgccgg cgaggcgtcg
     2341 ggcgccgagc ccacgtactg ggtgaagtgg tcgagcatct gcgccacccg cggctcgggg
     2401 acgtggcggc gcaccgtgcc ggccaccgtg cggccgaagc gcagcgacag caggtcggag
     2461 agcaccgacg ccttgaacgt ggtgccggcg tcgaaagtgt cgcgcaccga gcccacggag
     2521 cgccagaaga agtagcggtc cgagatcccg tgcagccgct cggccatgtc gaggaagcgc
     2581 ccgtagcccg cgtcggcgcc cgagccgggg gcgaaggcgg cgaggatctc cgccatgcgc
     2641 cgccggtcgg ccaccaggtc cagggccgac ccgtcgtcat agaagcagcg ccattgcggg
     2701 tccaggggga ccagctcgag ctcgtcccgc aggtcgcgcc ccgcctcgcc gaagatccgg
     2761 ccgagcaccg acggcagggt gaggatggtc ggccccatgt cgaagcggaa cccctgctcg
     2821 tggaggacgg cggccttgcc gcccagccag gggttgcgct cgaacaggac cacccgatgg
     2881 ccgcgggccg ccagggtgac cgccgcggcg agcccgccca gccccgctcc caggactccg
     2941 atccggtccg tcgtctctgt catagccccg cctctctggc cgccttgccg atgccgctgt
     3001 tgcccacacc ggccagcggc ggcgcggccg gggagcggtc cggcgctccg gaccggtgga
     3061 atggatgggc cgcggccgct ccgccgaagt cttgaagcaa cagccgggcc gtgatcagcg
     3121 ccgactcgaa gatcaccggc aggccgctcc cgggatgggt gccgccgccg accagataga
     3181 caccctccag gtcctcgaac cggttgcgcg gccgcaggtg cagcatctgg ccgaggttgt
     3241 gggcgaggtt gaaggtggcg ccgcggtgga tctcgaattc ctggtcccag tcgagcggcg
     3301 tcaccacccg ctcgtagcgg atgcggcgct cgacgtcctc gatccccagc ttttcgagct
     3361 gccgcagggc cacggcgcgg tagcggtcgc gctcctgccg ccagtccacg ttcgggtgct
     3421 ggtgggtgac cggcaccagg acgtacaggg tgctcatgcc cggcggcgcc aggcccaggt
     3481 cggtcaccga ggcgttctgc acgta
//
LOCUS       DASPJI010000618        20815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_438348, whole genome shotgun sequence.
ACCESSION   DASPJI010000618 DASPJI010000000
VERSION     DASPJI010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 20815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20815)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20815
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438348"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..809)
                     /locus_tag="VEL74_17230"
     CDS             complement(165..809)
                     /locus_tag="VEL74_17230"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HYG64324.1"
                     /translation="MIKINLLSEGKRPTAVRKTRKAAISLEGKEIGQWLLVTGIVLSI
                     LAVAAYWWTLKQRLEEVQSEVAAAEAEVQRLASVIKEVEDYKQKKAELERKIGIINDL
                     KANQRGPVRVMDYVSRALPELLWLDRVRMEASLIEVEGRAFNTNAVANFIENLDKVPE
                     FEEPTLKDTTQQGGGVYKFVISFNYSFSPPKAETDEEGAAGATTAPAATGRTTG"
     gene            complement(816..1877)
                     /gene="pilM"
                     /locus_tag="VEL74_17235"
     CDS             complement(816..1877)
                     /gene="pilM"
                     /locus_tag="VEL74_17235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HYG64325.1"
                     /translation="MFFTRSKKGVVGLDIGSSAIKLVELRERKGGEFHLQRLGIEPLS
                     PEAIVDGSIMDSSLVVDAIHGLNDTTKVKNTSYATSLSGHSVIIKKIQLPTMPEEELA
                     ESIQWEAEQYIPFDINDVRLDYVVLGDGEPGRDTMDVLLVAVKRDKVNDYVSVISQTG
                     KTASIVDVDAFAVQNAYEANYQLDPRKTLALINMGAGVTTINILARGTTVFWRDISFG
                     GNQFTEALQREFNLSFAQAESLKRGEPVDRYSASDARPVLDTVSAEMASEIRKTFDFF
                     SATSSEGPVDELVLSGGCALTPNLLQVLRERFEVPTELMNPLRQIHFKESDFNGDWLR
                     SIAPMMAVAVGLGIRKVGE"
     gene            2413..2649
                     /gene="rpmB"
                     /locus_tag="VEL74_17240"
     CDS             2413..2649
                     /gene="rpmB"
                     /locus_tag="VEL74_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HYG64326.1"
                     /translation="MAKNCEVCGKTTVFGRNISHAHNVTPRTWEPNLQTVRALVDGAT
                     RRIRVCTRCLRSGKVVKAPARKWTPEESGTPAKG"
     gene            complement(2794..5025)
                     /locus_tag="VEL74_17245"
     CDS             complement(2794..5025)
                     /locus_tag="VEL74_17245"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HYG64327.1"
                     /translation="MPTTKNLAPPVQARTATRLDEVVACLDGHGRRVDRDFLQRVYTF
                     SEKMHQGQTRRSGEPYMIHPLNVAYLLAELNFDQTCVAVGLLHDVLEDTLSSREVLES
                     EFGAEVTELVDGVTKIGRHEYVRRDEAQAETFRKMILASAKDIRVIVVKLADRLHNMQ
                     TLQHMPPDSRRRISRETLEIYSPIAHRLGMARVKGDLEDLAFFYLYPHQFGELHSKIA
                     EKMKMGQGATQRIRERFARSLEAAGIDAEISFRVKRYYSIYQKLRRQGIDISQLYDYL
                     AFRIVTGSLKDTYAALGVVHQNWRPIPGRFKDYIAMPKPNLYQSLHTTVVGDQGQPFE
                     VQIRTREMDLVAEEGIAAHWRYKEGRVTNGESDPNILWLRQLLEWQKEVQDPRTFLTT
                     LKVDLYPDEVYVFTPKGQVLSFPRGATPLDFAYKVHTDLGHHCAGARVNARLVPLRTP
                     LQNGDMVEILTNPNRHPSRDWLNFAGTSRAKSKIRQWLNTQQKQRATEIGRRLVEKEL
                     RRYGVSLRKVVDHPQFAVYLQEEGLARVEDLYSRIGFGKTEARQVLTRVLGEEQVTAP
                     PREPSRLRQAVSRILPFGTAGPITVKGHGDLLAYLAKCCNPLPGEEIVGYVTRGRGVS
                     VHSVDCPNVRNLLYNPEREIEVEWDRQRDDTYQVSFLIETEDQPGVLARLAETISKTG
                     SNISQMEVETHDETGRASIGVVCALRNRKHMDKLLRDVQALAGVLRVERRMNAGRTAE
                     ALG"
     gene            complement(5229..6239)
                     /gene="secF"
                     /locus_tag="VEL74_17250"
     CDS             complement(5229..6239)
                     /gene="secF"
                     /locus_tag="VEL74_17250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HYG64328.1"
                     /translation="MEILRDTNIDFMKYRKVWVLVSLALIAVAIFSVFVHGQLNIGVD
                     FAGGTQMTVKFQQRPDVQRLREVIAGAGIPEPQIQTYGREAENQVLVKTRLIEGSAEG
                     SRERVETALRQANMGEFSILSVESVGPQIGRELRRQGFWAVALSLLGMLVYIWLRFEL
                     RFGIGAIMACVHDIIVTLGLFALFDFEFNLTTIAAFLTVVGYSVNDTVVIFDRIRENM
                     RKTRREPLIDVMNKSINQTLSRTILTGGSTLLVLACLLYWGGEAIRGFAFVIFIGIIV
                     GTYSSIYIAPPFTLLWERYFGNRGNKIRGQGAPLPAGGAAPAAPARTETPATRRAGGA
                     RR"
     gene            complement(6268..7839)
                     /gene="secD"
                     /locus_tag="VEL74_17255"
     CDS             complement(6268..7839)
                     /gene="secD"
                     /locus_tag="VEL74_17255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HYG64329.1"
                     /translation="MKRNLLWRGLLILAVLVGAAAIAFPPQEKINLGLDLRGGMHLVL
                     QVQTVDALKAETDSEMARLARIAEDEGVAGLRPRRTGDTTFEVAGLSPQAKDTLDQIA
                     DDSFAGAWEAREEGDRMVFTMTDQNSSQIRQLSVTQAVQTIRNRIDAFGVAEPVIQEM
                     GEERILVQLPGVDDPERVRNLIKSTALLEFRIVRFPQGGGGGVPSREAILANYNNRLP
                     EDLEILEGDVRDAEGRVTQKVYYAVDKRRDVTGRDLKTARPGLGQFNDPIVEFSLTPE
                     GAQKFGALTGANTGQGLAIVLDNRVVSAPVIRSRITDSGIIEGNFTQQEVQDLSTVLR
                     SGALPASLTYLEERTVGPSLGRDSIESGLLAGLIGTALVVLYMLIIYNLSGVNAVIAL
                     ALNLLLLFGGLAAFGANLTLPGIAGIILTIGMAVDANVLVFERIREELHAGRTVRSAV
                     DLGYEKALSSIIDSNVTTLVAALFLFQFGTGPIRGFAVTLTIGLLASMFTAVFVSRWL
                     FDFVLSRRQVQRLSI"
     gene            complement(7836..8165)
                     /gene="yajC"
                     /locus_tag="VEL74_17260"
     CDS             complement(7836..8165)
                     /gene="yajC"
                     /locus_tag="VEL74_17260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HYG64330.1"
                     /translation="MNPFLAMPLALQAGDTQSTLVAFAPMLIIFAIFYFLLVMPMRKR
                     QKALAQTIEALKKGDKVLTTGGLYGEVAGVEPTSLILKIADNVKVRVAKSAIAGLEAE
                     AEKGSPS"
     gene            complement(8192..9367)
                     /gene="tgt"
                     /locus_tag="VEL74_17265"
     CDS             complement(8192..9367)
                     /gene="tgt"
                     /locus_tag="VEL74_17265"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HYG64331.1"
                     /translation="MRFDILANDGEARLGRLTTPHGVVETPAFMPVGTLGAVKGVPAA
                     DLAAQGASIMLSNLYHLALRPGIDTIERLGGLHGFNGWPGPILTDSGGFQVFSLDGLR
                     TVDAGGVTFRSHVDGSPMRFTPEGVVEMQERMGVDIAMVLDECPPWPIDEAAATRSWE
                     RTLDWARRSRDQSSGGPGGLFGIVQGSVYRSVRERAAADLSRLGFDGHAIGGVSVGEP
                     APHRRAVVEWTAPVLPAERPRYLMGVGYPADIAHAVNHGVDLFDCVLPSRNARHGTLF
                     TRQGVLKIRNAQYREDPRPVDPECGCPLCARHSRAFLHHLVRTGEITAQILATLHNLR
                     YYLDFMAALRQAIRLGTLADLTAPFAGRAAGAVHPAVPPDDPLAPPVAADPIEQEIF"
     gene            complement(9381..10148)
                     /locus_tag="VEL74_17270"
     CDS             complement(9381..10148)
                     /locus_tag="VEL74_17270"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HYG64332.1"
                     /translation="MKKHLTATLSALLLAASALIATPAVAAAPPDPMSPSVPKTARLQ
                     ALLERVKYEQKALKTMEARFVQQQESSMLAEPEESSGVFSYSAPDSVRWEYQAPSPIT
                     VVIRGDDMTTWYRDLNRADKLKVGRYSSQFFKYLGASGNMQTLLDYFTVKLQVPAKKG
                     DPYRLEMTPRYARIAKRLKSMTLWIDSEMFFPTRLEYVEADGDSTRYRFENVRRNVSI
                     PAERFVLQLPRGVQTRVIDLGEKGRDPANPIKTPAKP"
     gene            complement(10218..11012)
                     /locus_tag="VEL74_17275"
     CDS             complement(10218..11012)
                     /locus_tag="VEL74_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64333.1"
                     /translation="MNTLLDGLVMAALLAAWGAQAVAPWRLASRLPGRPEEARRFWGY
                     TILPLYAGGVLAALSWAHTHPDATVAAGLVPITSSPWGRALAILAVALLAADLVAVVG
                     RRRLEDAGWRILGGFGLAALAAVCLVAELLRAGEGPVSPLPVTTLAAACRLLVALAAG
                     EALAPFGAGGRPLLALAAAPALGAWFFVVPAGLADALVRSGALLTAGAAAVLFLAARW
                     LPASLRRPGLAAAALLAGLFFAQAAAVSQALGAPVFSPLPPLPGPG"
     gene            complement(11023..11526)
                     /gene="folK"
                     /locus_tag="VEL74_17280"
     CDS             complement(11023..11526)
                     /gene="folK"
                     /locus_tag="VEL74_17280"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017871394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HYG64334.1"
                     /translation="MTPVTVALALGGNEGAVEQTLRLALRQLRASLGPLVAAPLYRSR
                     AVSPIPQPDYLNTAALARTALPPEAVLALAKALERAAGRRRGPRFGPRPLDIDLLLYD
                     GLLSDSAEMTLPHPRLRERRFVLEPLAEIAPDLPVPPDGAPVRELLARVAPEPLERVL
                     GRGWDLL"
     gene            complement(11552..11857)
                     /locus_tag="VEL74_17285"
     CDS             complement(11552..11857)
                     /locus_tag="VEL74_17285"
                     /inference="COORDINATES: protein motif:HMM:NF021083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FYDLN acid domain-containing protein"
                     /protein_id="HYG64335.1"
                     /translation="MPELKLGAKYECFNCGTKFYDLGKAEPLCPKCGANQKDATPDTP
                     AVSQASRRKRKAEVAPRPLDGDEDDAPVEIPDDDIEAADIDADLDEDDDEEEFDEAE"
     gene            complement(11956..12861)
                     /locus_tag="VEL74_17290"
     CDS             complement(11956..12861)
                     /locus_tag="VEL74_17290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HYG64336.1"
                     /translation="MPRPSSPATPDSSRNQPSLQPPRAPLVAVAAAALLVLLKGAGAW
                     ITGSLALGSAALDSLIDVFISAANYVVLRRSAAPPDADHAYGHGKFENLAALGQGLFL
                     MTAAVSLVVAGVGRLSSGEAPRQTGLGAAVLVLSLLVSLGVARLLRREARRTESPALA
                     ADSLHYQTDLWVNGAAVAALVIVRWTGWAPADPLVAMGVAVLVFRSGGALVLESVGVL
                     SDRALPAEELRRIEEVVASFAPDVVGLHDLKTRRGGGQRFIELHLEIPRGKSFEEAHD
                     LMVRVLRAIEDELPRSKVFVHGDPV"
     gene            complement(12827..13705)
                     /locus_tag="VEL74_17295"
     CDS             complement(12827..13705)
                     /locus_tag="VEL74_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64337.1"
                     /translation="MWKTPALPRLLLPAAVLVALVVAGAPLAAQIPAGDAAWARRAEG
                     HRGARAAAGPAGEAVAAYERAVRAEPGNLEAQWKLMRALHFQGEHVARTKEERQAAFD
                     RGKSVAEKAIDLAARQAGGRAKLDALSPQQAAKALAGTRDATPVFLYAAIHWGLWGDS
                     FGRIAAARQGVGDKIRRYGEIVVALDERYETAGGHRLLGRLHTLAPKVPLVTGWVDRG
                     KAVSELRRALALFPEDPYNQLFLAEALLEHQPDKSAEARTILRRLAARQPAADRVVEE
                     EKAIADAKALLARHPG"
     gene            13754..14452
                     /locus_tag="VEL74_17300"
     CDS             13754..14452
                     /locus_tag="VEL74_17300"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HYG64338.1"
                     /translation="MADRSDSKPRLPLVPLPDVVHFPRTALKIRVADPRYLRLVRDLL
                     EQDEEVRWAGVVLLKPGWVRDAAGRPEIFPGGTAGRLVEAEPQADGTTQILLLGDFRF
                     ELEAELPGDRPYRSALVRPVQEPWLNEQDPGILAVRRSLVELAGALATELGERFPFDA
                     GEIEDLAERSDFEELINRLAAGFDLPPLRKLQLLSEALPERGESLLSILRHRQQVLDS
                     LRPYRHLAGGSEHN"
     gene            14462..15679
                     /locus_tag="VEL74_17305"
     CDS             14462..15679
                     /locus_tag="VEL74_17305"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HYG64339.1"
                     /translation="MLSIRDPVHGFIRADPLEAALINSRPVQRLRFIHQLGFTFLVFP
                     GAEHSRFSHVLGAMHLAGRVYDALCSRAEGLLPPGTASRERRLVRAAALVHDLGHAPF
                     SHSAEGLFEDGIDHEEMTRRLLRAPEIEEVFARHGAGITTGDVARLLAGGGDPAERLL
                     AQIVSGELDIDKMDYLLRDSLYCGVRYGSYDLDRLLDTMLPIADPDTGDWGVGVDEGG
                     VHALEALVMARYYMFTQVYFNVTGKALELHLNEWLLESGIRWPAEPELFLEQDDVSVW
                     AAMRASRSPHARAVVAREHFPVAFETREHLTREEKERFESLLPALRERFGAGNLLLSN
                     SAKDPHRLGRQRVLVRRFDGSLEPMEEASQFIRHLSRIDRYRVYTPPPLREAVAAGFR
                     ERWEPSASPDSNS"
     gene            15927..18785
                     /locus_tag="VEL74_17310"
     CDS             15927..18785
                     /locus_tag="VEL74_17310"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYG64340.1"
                     /translation="MLAQGVPTGSVSGRVTSAEGTALPGVTVTVTSPQLQGTRSATTS
                     AQGDYNLPLLPPGDYQVNYELAGFLTAEHSVKVAGGLQARIDVELAPNSVSEEIVVTG
                     TYETISTGSQDATTYEKEFIEQLAIDRDIRNTTLLTPGVSATGPTNAITISGALSYEN
                     LFLVNGVAVNENLRGQPYNLFIEDAIDETTISTSGISAEYGRFGGGVVNTITRSGGNE
                     LHGSLRTVFLNDDWIGRTPVSPERLDDINQRYEATLGGWVLKDRVWYFLAGRDYELSG
                     SSATALTQIPYETGESETRLEGKLTVSPFQGHRLIGSYMEIEREETNARFQSILDIES
                     LKSRTLPNELRALNYTGVLTESFFVEAQYSERFFAFEGDGSKFTDRIRGTLLIDDELH
                     RWNSPTFCGICRPEERNNENIYAKASWFASTESLGSHDIAFGYDSFDDIRVADNHQSG
                     SDFRVNLSDTWIIGTNLYPQLLNDNTTIITWNPILQGSRGTSFVTNSIFANDRWRLNE
                     NWSFNLGVRYDQNDGKDSSGKKVADDSRISPRLGLTFDPKGDGDWVFNASYGQYVSAL
                     ANTQGDATSAAGNPANITWFYRGPNINPDGTPADQLISTEEALRRMFEWFDQQGGTNY
                     AVHRPRSLTIPGETSRIVDGLKSPVTDEIALGVSKRLGNRGIARAEYVHREGHDFYVD
                     RRDLTTGQVTLSNGSRADLSVIENEDDLLERTYDGLHTQFQYRVGDRLSLGGNYTLSF
                     LKGNWTGENSASGPVRSTILQYPEYIDVDWFAPEGYLSADQRHRARVWGLFDLFKTDH
                     HRLNLSVLQLYTSGTPYGALGAVRSLNYNDNPGYAIEPTSVAYFFTDRDEFTTEDITS
                     TDLALNYSFTWNAFNKSMEVFIQPEVTNVFNEDGAILVDQTVRDNTTTTTIPSFNPFT
                     ETPVEGVHWQRGANFGKAVNETGFQTPRTFRFSVGFRF"
     gene            complement(18844..19320)
                     /locus_tag="VEL74_17315"
     CDS             complement(18844..19320)
                     /locus_tag="VEL74_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64341.1"
                     /translation="MREAREAIRQGDIPGVQLRRRQDLALGPAEAWRWLVEPERLCLW
                     LAEEARIAGDREEELLLRITGDRGEPVRERGRILGTIPATLWVLSFERLEAGWTAPTR
                     LTLRLAATPAGCELDVLQEGFQRLPLSLGLTAWESYRVRWRTALARLAAAVPLRSA"
     gene            complement(19327..20262)
                     /locus_tag="VEL74_17320"
     CDS             complement(19327..20262)
                     /locus_tag="VEL74_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HYG64342.1"
                     /translation="MRVVITGGSGLIGSALARELGGAGHDAVVLSRNLSRVGALPPGV
                     RAAEWDGRSGQGWSKLLDAGTAIVHLAGESIASGRWTAEKKRRIRDSRVQSGKAVLEA
                     VRQAASSSAGAPAVLLQGSAVGYYGPCGDEVVTEDHPSGNDFLADVCREWEASTTEVE
                     ALGVRRALLRTGIVLSLKDGALPRMMLPFRLGAGAPLGSGRQWFPWIHMADEVAAIRF
                     LLERREASGPFNLSAPNPVTNRQLGDVLSRVVRRPNPLQALGLGVPRAVFQVALGEMA
                     GMLLQGQRAVPRRLLDLGFPFRYPELEPALRNLVD"
     gene            complement(20319..>20815)
                     /locus_tag="VEL74_17325"
     CDS             complement(20319..>20815)
                     /locus_tag="VEL74_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYG64343.1"
                     /translation="QESFLFSASIRENIGFGRPGATDDEILEAARLAGLGSDLSQFPN
                     GLDTIVGERGITLSGGQKQRVALARALLRRPRILLLDDCLSAVDTHTEEQILRNLRTV
                     FEGRTVFLVSHRISTVKDADLILVLDQGRIAERGTHAQLLAHGGLYADLHQRQLLEEE
                     LAAV"
BASE COUNT         3179 a         7221 c         7295 g         3120 t
ORIGIN      
        1 ccttccagct tggcctcctc ggccaggatc tcttctttct gtggccccag caggcgccag
       61 tagccgaggc cgaagaggat ggcgccgacg ccgatgccgg ccgccaggcc gaagtaccag
      121 ggctttccct cgagtccggt cttgatcgcc acggtgtcgt ctccttaacc cgtggtacgc
      181 ccggtggccg cgggagcggt ggtggcgccg gcggcgccct cctcgtccgt ctcggccttg
      241 ggggggctga atgagtagtt gaagctgatg acgaacttgt agacgccgcc accctgctgc
      301 gtggtgtcct tcagggtggg ctcctcgaac tcgggcacct tgtcgaggtt ctcgatgaag
      361 ttggccaccg cgttggtgtt gaaggcgcgc ccctcgacct cgatcaggct cgcctccatg
      421 cgcacgcggt cgagccagag cagctcgggc agcgcccggg agacgtagtc catcacccgc
      481 accgggcccc gctggttggc cttgaggtcg ttgatgatgc cgatcttcct ctccagctcg
      541 gccttcttct gcttgtagtc ctcgacctcc ttgatgacgg aggcgagccg ctggacctcg
      601 gcctcggcgg cggcgacttc ggactgcacc tcttccagcc gctgcttgag cgtccaccag
      661 taggcggcga cggccaggat gctgaggacg atgccggtga ccagcagcca ctggccgatc
      721 tccttgcctt cgagcgagat cgcggccttg cgggtcttgc ggaccgcggt ggggcgtttg
      781 ccctcggaga gcaggttgat cttgatcatg ggcgtctact cccccacctt gcggatgccg
      841 agacccacgg cgacggccat catcggcgcg atggagcgca gccagtcgcc gttgaagtcg
      901 ctctccttga agtggatctg gcgcaagggg ttcatcagtt cggtcggcac ctcgaagcgc
      961 tcccgcagca cctgcagcag gttgggcgtc agcgcgcagc cgccggagag caccagctcg
     1021 tccaccggcc cttccgacga ggtggcagag aagaagtcga aggtcttgcg gatctccgac
     1081 gccatctccg ccgacaccgt gtcgagcacc ggccgggcat ccgaggccga gtagcggtcc
     1141 accggctcac cgcgcttcag gctctcggcc tgggcgaagg agaggttgaa ctcgcgctgc
     1201 agcgcctcgg tgaactggtt gccgccaaag gagatgtcgc gccagaagac ggtggtgccg
     1261 cgagccagga tattgatggt ggtgacgccg gcccccatgt tgatgagcgc cagggtcttc
     1321 ctgggatcga gctggtagtt ggcctcgtac gcgttctgca ccgcgaacgc gtcgacgtcc
     1381 acgatggagg cggtcttgcc ggtctggctg atcaccgaga cgtagtcgtt gaccttgtcc
     1441 cgcttgaccg ccaccagcag cacgtccatc gtgtcccgtc cgggctcgcc gtcgccgagc
     1501 accacgtagt ccaggcggac gtcgttgatg tcgaacggga tgtactgctc cgcctcccac
     1561 tggatcgact cggccagctc ctcctccggc atggtgggga gctggatctt cttgatgatc
     1621 accgagtgtc ccgacaggga cgtcgcgtag ctggtgttct tgacctttgt cgtgtcgttg
     1681 aggccatgga tggcatcgac caccagcgac gagtccatga tcgacccgtc gacgatggcc
     1741 tccggcgaca gcggctcgat accgaggcgc tggaggtgga actcaccccc cttgcgctct
     1801 cgcagctcca ccagcttgat ggccgagctg ccgatgtcca ggcctactac acccttcttg
     1861 gagcgagtga agaacacttt ggagctcccc cagaaaagcc cagcattgga cgctacttaa
     1921 cggcacttag ttaaagcttc gctggaagct accaggaaac cacaccggtg tcaaggcgtc
     1981 tatacgtcta tggctatatt gaccagtgca ggaactctac accaggtccg aacttcgtgc
     2041 aagcgctaga tttgggggaa atcggcggcg agcccgcgag gggttgtagt gccgaatggc
     2101 gccggacgac gtccggggct cccgggagct tcccgaacgc tccggagtct atccggacgg
     2161 cctggacgcc ttccggaagg ccgggacgct tcccgggccg tacgggctcg atccttctca
     2221 ccgggacgcc gtccttctca ccgggacgcc atccaggacg tccgggaagt cacccagagc
     2281 gtccggagcg cgtccgcggc gcccggacgc gatccggcgg gaacggttgc cccccgaacc
     2341 ctgttctgct attctgacgt gcctttcggg gcaagcctcg tctaggcgaa tcgtagtcag
     2401 gagagagaca tcatggccaa gaactgcgaa gtctgcggca agaccacggt cttcggtcgc
     2461 aatatcagcc acgcccacaa cgtgactccg cggacctggg agcccaacct gcagaccgtc
     2521 cgcgctctgg tcgacggcgc cacgcgccgc atccgcgtct gcacccgctg cctgcgcagc
     2581 ggcaaggtgg tcaaggctcc ggcccgcaag tggacccccg aggagagcgg gacgccggcc
     2641 aagggttagc cacccgccgt cgctcccccg cctcggactc caaccccacc cgccaatcca
     2701 tcgagtgctc cggggtctac ttcccccctg taggggaggg gcttgccccc tcccgggagg
     2761 gggcaagccc ctcccctaca ttcttcttgg ggatcagccc agcgcttccg ccgtgcggcc
     2821 ggcgttcatg cggcgctcca cgcgcagcac gccggccagc gcctggacgt cgcgcagcag
     2881 cttgtccatg tgcttgcggt tgcgcagcgc gcagacgacg ccgatcgagg cgcggccggt
     2941 ctcgtcgtgc gtctcgacct ccatctggct gatgttgctg ccggtcttgc tgatcgtctc
     3001 ggcgaggcgg gcgagcaccc ccggctggtc ctcggtctcg atgaggaacg acacctggta
     3061 ggtgtcgtcg cgctggcggt cccactccac ctcgatctcc cgctccgggt tgtagagcag
     3121 gttgcggacg ttcgggcagt ccaccgaatg gacggagacg ccccggccgc gcgtgacgta
     3181 gccgacgatc tcctcgcccg gcagcgggtt gcagcacttg gcgagatagg ccagcaggtc
     3241 gccgtgcccc ttgaccgtga tcgggccggc ggtgccgaac ggcaggatgc ggctgaccgc
     3301 ctggcgcagc cggctcggct cgcgcggcgg cgcggtgacc tgctcctcgc ccagcacgcg
     3361 ggtgagcacc tgccgcgcct cggtcttgcc gaagccgatg cggctgtaga ggtcctccac
     3421 ccgcgcgagg ccctcctcct ggagatagac cgcgaactgc gggtggtcca ccaccttgcg
     3481 cagcgacacg ccgtagcgcc gcagctcctt ctccaccagc cgccggccga tctcggtcgc
     3541 ccgctgcttc tgctgcgtgt tcagccactg gcggatcttg ctcttggcgc gcgaggtgcc
     3601 ggcgaagttc agccagtcgc ggctcggatg ccggttcggg ttggtgagga tctccaccat
     3661 gtcgccgttc tggagcggcg tgcgcagcgg caccagccgg gcgttgaccc gcgcgccggc
     3721 gcagtgatgc ccgaggtcgg tgtgcacctt gtaggcgaag tcgagcggcg tggcgccgcg
     3781 cggaaaggag agcacctgcc ccttgggcgt gaacacgtag acctcgtccg gatagaggtc
     3841 gaccttgagc gtggtgagga aggtgcgggg gtcctgcacc tccttctgcc actccaggag
     3901 ctggcgcagc cagaggatgt tggggtcgct ctcgccgttg gtgacccggc cctccttgta
     3961 gcgccagtgg gcggcgatcc cctcctcggc caccaggtcc atctcgcggg tgcggatctg
     4021 cacctcgaac ggctgcccct ggtcccccac caccgtcgtg tgcagcgact ggtagaggtt
     4081 gggcttgggc atcgcgatgt agtccttgaa ccggcccggg atcgggcgcc agttctggtg
     4141 caccacgccc agggcggcgt aggtgtcctt gaggctgccg gtgacgatgc ggaacgccag
     4201 gtagtcgtag agctgcgaga tgtcgatccc ctgccgccgc agcttctggt agatcgagta
     4261 gtagcgcttg accctgaagc tgatctcggc gtcgatgccg gccgcctcga ggctgcgggc
     4321 gaagcgctcg cggatgcgct gcgtcgcccc ctgccccatc ttcatcttct cggcgatctt
     4381 cgagtggagc tcgccgaact ggtggggata gaggtagaag aacgcgaggt cctccaggtc
     4441 ccccttgacg cgcgccatgc ccagccggtg cgcgatcggc gagtagatct ccagcgtctc
     4501 gcgcgagatg cggcgccgcg agtcgggggg catgtgctgc agcgtctgca tgttgtgcag
     4561 gcggtcggcg agcttgacca cgatcacccg gatgtccttg gcggaggcca ggatcatctt
     4621 gcggaaggtc tccgcctgcg cctcgtcgcg gcgcacgtac tcgtggcggc cgatcttggt
     4681 gacgccgtcg accagctcgg tgacctcggc cccgaactcg ctttccaaga cctcgcggct
     4741 ggagagggtg tcctccagca cgtcgtggag cagcccgacg gcgacgcagg tctggtcgaa
     4801 gttgagctcc gccagcagat aggcgacgtt caacgggtgg atcatgtagg gctcgcccga
     4861 ccggcgggtc tgcccctggt gcatcttctc ggagaaggtg tagacccgtt gcaggaagtc
     4921 ccggtccacc cggcgaccgt ggccgtcgag acaggcgacg acctcgtcga ggcgggtggc
     4981 ggtgcgcgct tgcaccggtg gcgcgaggtt cttcgtcgtc ggcatggcca ggggcctccg
     5041 ccggagcggg cccgcggtca gcgagggtgc gcccggcggg aagggtgaac gattatagag
     5101 cagcggagcg gctgagcacc gcctgcttct cacaacgggc ggttgtcaca cggaatttcc
     5161 ccaaaacgaa aacccgcccc accctcgaag ggcggagcgg gctggtgaga ggccggaggg
     5221 ccgtcgtgct accggcgggc gccgccggcg cgccgggtcg ccggcgtctc ggtgcgggcc
     5281 ggcgcggcgg gcgccgcgcc gccggccggc aggggcgcgc cctggccgcg gatcttgttc
     5341 ccccggttgc cgaagtaccg ctcccagagc agggtgaacg gcggggcgat gtagatcgac
     5401 gagtaggtac cgacgatgat gccgatgaag atgacgaagg cgaagccgcg gatcgcctcg
     5461 ccgccccagt acagcaggca ggcgagcacc agcagcgtcg agccgccggt gaggatggtg
     5521 cgcgacagcg tctggttgat gctcttgttc atcacgtcga tcagcggctc gcggcgggtc
     5581 ttccgcatgt tctcgcggat gcggtcgaag atgaccaccg tgtcgttgac cgagtagccg
     5641 acgacggtga ggaaggcggc gatggtcgtc agattgaact cgaagtcgaa gagcgcgaac
     5701 aggcccagcg tcacgatgat gtcgtggacg caggccatga tggcgccgat gccgaagcgc
     5761 agctcgaaac gaagccagat gtagaccagc atgccgagca gcgacagggc gacggcccag
     5821 aagccctggc gccgcagctc ccggccgatc tgcgggccga cggactcgac gctcaggatg
     5881 ctgaactcgc ccatgttggc ctgccgcagg gccgtctcga cccgctcgcg gctcccctcc
     5941 gccgatccct cgatcagccg cgtcttgacc aggacctggt tctccgcctc gcggccgtag
     6001 gtctggatct ggggctcggg gatgccggcg ccggcgatca cctcacgcag gcgctggacg
     6061 tccggccgct gctggaactt cacggtcatc tgggtgccgc cggcgaagtc gacgccgatg
     6121 ttgagctgcc cgtggacgaa gaccgagaag atggcgacgg caatcaaggc cagggacacg
     6181 aggacccaga ccttccggta cttcatgaag tcgatgttgg tatcgcgcag gatctccatg
     6241 gtgtgctcct tgagcgggcg gtgccctcta gatcgaaagc ctctggacct ggcgccgcga
     6301 caggacgaag tcgaaaagcc agcggctgac gaagacggcg gtgaacatgg aggccaggag
     6361 accgatggtc agggtgacgg cgaagccgcg gatcggcccg gtgccgaact ggaacaggaa
     6421 gagggcggcg accagcgtcg tgacgttgga gtcgatgatg gacgacagcg ccttctcgta
     6481 gccgaggtcc accgccgacc ggacggtcct gccggcgtgc agctcttccc ggatacgctc
     6541 gaagacgagc acgttcgcgt cgaccgccat accgatggtc aggatgatgc cggcgatgcc
     6601 gggcagcgtc aggttggcgc cgaacgccgc caggccgccg aacagcagca ggaggttgag
     6661 cgccagggcg atcaccgcgt tgacgccgct caggttgtag atgatcagca tgtagaggac
     6721 gaccagggcg gtgccgatga ggccggccag caggccgctc tcgatcgagt cgcggccgag
     6781 cgacgggccg acggtgcgct cctccaggta ggtcaggctg gcgggcagcg cgcccgaccg
     6841 caggacggtg gacaggtcct gcacctcctg ctgggtgaag ttgccctcga tgatgccgct
     6901 gtcggtgatg cgggagcgga tgaccggcgc cgagacgacg cggttgtcga gcacgatggc
     6961 gagcccctgg ccggtgttgg cgcccgtcag cgcgccgaac ttctgggccc cctcgggggt
     7021 caggctgaac tcgacgatcg ggtcgttgaa ctggccgagg ccgggccgcg ccgtcttgag
     7081 gtcgcggccg gtgacgtcgc ggcgcttgtc caccgcgtaa tagaccttct gggtcacgcg
     7141 cccttccgcg tcgcggacgt ccccctcgag gatctcgagg tcctccggca gccggttgtt
     7201 gtagttggcg aggatcgcct cacggctcgg cacgccgccg ccgccgccct gggggaagcg
     7261 gacgatgcgg aactcgagga gcgccgtgct cttgatcagg ttgcggacgc gctccgggtc
     7321 gtcgacgccg gggagctgga cgaggatgcg ctcctccccc atctcctgga tgaccggctc
     7381 ggcgacgccg aaagcgtcga tgcggttgcg gatggtctgc accgcctggg tgaccgagag
     7441 ctggcggatc tgggagctgt tctggtccgt catggtgaag accatgcggt ccccctcctc
     7501 ccgcgcctcc caggcgccag cgaaggagtc gtcggcgatc tggtccaggg tgtccttggc
     7561 ctggggcgac aggcccgcca cctcgaaggt ggtgtcgccg gtgcgccgcg ggcgcaggcc
     7621 ggccaccccc tcgtcctcgg cgatgcgggc gaggcgcgcc atctcggagt cggtctccgc
     7681 cttgagcgcg tcgaccgtct gcacctggag caccagatgc atgcccccac gcaggtcgag
     7741 gccgaggttg atcttctctt gcggcggaaa agcgatcgcc gccgcgccga cgagcacggc
     7801 caggatcaac aggccgcgcc acagcagatt gcgtttcatg acggactccc tttttcggcc
     7861 tcggcctcga ggccggcgat ggccgacttg gccacccgca ccttgacgtt gtctgcgatc
     7921 ttgaggatca gggacgtggg ctcgacgccc gccacctcgc cgtacaggcc gccggtggtc
     7981 agcaccttgt cgcccttctt cagcgcctcg atggtctgtg ccagcgcctt ctgccgcttg
     8041 cgcatcggca tgacgaggag gaagtagaag atggcgaaga tgatgagcat cggggcgaag
     8101 gccacgaggg tgctctgggt gtcaccggcc tggagggcga ggggcatcgc gagaaatgga
     8161 ttcatggtct atacccggca ggaaaacctg ctcaaaagat ctcttgctcg atgggatcgg
     8221 ccgcgaccgg cggggccagc gggtcgtccg gagggactgc cggatggact gcgccagccg
     8281 cacggccggc gaatggcgcc gtcagatccg cgagcgtacc gagtctgata gcttgccgga
     8341 gcgcggccat gaagtcaagg taataccgca ggttgtgcag cgtcgccagg atctgggcgg
     8401 tgatctcgcc ggtgcgcacc aggtgatgca ggaaggcgcg cgagtggcgg gcgcagaggg
     8461 ggcagccgca ctccggatcg accggccggg ggtcctcccg gtactgggcg ttcctgatct
     8521 tgagcacccc ctggcgggtg aacagggtgc cgtggcgggc gttgcgcgac ggcagcacgc
     8581 agtcgaagag gtcgacgccg tggttgacgg cgtgggcgat gtccgccgga tagcccaccc
     8641 ccatgaggta gcgcgggcgc tcggccggca gcaccggcgc ggtccactcc accaccgcgc
     8701 ggcggtgcgg cgccggctcg ccgacgctga cgccgccgat ggcatggccg tcgaacccca
     8761 gccgcgacag gtccgccgcg gcccgctctc gcacgctccg gtagacgctc ccctggacga
     8821 tgccgaacag cccgccgggc cccccgctcg actggtcgcg cgagcggcgc gcccagtcga
     8881 gcgtgcgctc ccaggagcgg gtggccgccg cctcgtcgat cggccacggg gggcactcgt
     8941 cgagcaccat ggcgatgtcc acccccatgc gctcctgcat ctccaccacc ccctcggggg
     9001 tgaagcgcat cggcgagccg tcgacgtggc tgcggaaggt gacgccgccg gcgtcgacgg
     9061 tgcgcaggcc gtcgaggctg aacacctgga agccgccgct gtcggtgagg atcggcccgg
     9121 gccagccgtt gaagccgtgg aggccgccga ggcgctcgat ggtgtcgatg cccggccgca
     9181 gcgccaggtg gtagaggttg gacagcatga tggaggcgcc ctgggcggcg aggtccgccg
     9241 ccggcacccc cttgaccgcg ccgagcgtcc ccacgggcat gaacgccggc gtctcgacca
     9301 ccccgtgcgg agtggtcaga cggcccaggc gcgcctcgcc gtcgttggcg aggatgtcga
     9361 aacgcagcgg cacgggagga tcagggtttg gccggcgttt ttatcggatt ggcgggatcg
     9421 cggcccttct cccccaggtc gatcacccgg gtctggacgc cgcgagggag ctggagcacg
     9481 aagcgctccg ccgggatgga gacgttgcgg cggacgttct cgaagcggta ccgggtgctg
     9541 tcgccgtcgg cctcgacgta ctccaggcgg gtggggaaga acatctccga gtcgatccac
     9601 agggtcatcg acttgagccg cttggcgata cgggcgtagc gcggcgtcat ctccagccgg
     9661 tagggatccc ccttcttcgc cggcacctgc agcttgacgg tgaagtagtc gagcagcgtc
     9721 tgcatgttgc cgctcgcccc caggtacttg aagaactggc tggagtagcg ccccaccttg
     9781 agcttgtcgg cgcggttgag gtcgcggtac caggtggtca tgtcgtcgcc gcggatgacc
     9841 acggtgatgg ggctgggcgc ctggtactcc cagcgcacgc tgtccggagc ggagtaggag
     9901 aagacgccgg aggactcctc gggctccgcc agcatggagc tctcctgctg ctggacgaag
     9961 cgggcctcca tggtcttgag cgccttctgc tcgtacttga cccgctcgag cagggcctgc
    10021 aggcgggcgg tcttggggac cgagggggac atggggtccg gcggagcggc cgcgactgcg
    10081 ggagtggcga tcagggccga agcggcgagg agcagggcgg acagggtcgc tgtgaggtgc
    10141 ttcttcatgt cttggattcc aatgccaggg gagccaacat ccggggcggg ccgggcattc
    10201 catgctacca ccgctcccta ccccgggccg ggcagcggcg gcagggggga gaagacgggg
    10261 gcgcccagcg cctgcgagac ggcggcggct tgggcgaaaa agaggccggc cagcagggcc
    10321 gccgccgcga gcccggggcg gcgcagcgac gccggcagcc agcgggcggc gaggaacagc
    10381 acggcggcgg cgccggcggt gagcagcgcg ccgctccgca ccagggcgtc cgccaggccg
    10441 gcggggacga cgaagaacca ggcgcccagg gcgggggcgg cggccagggc gaggagcggc
    10501 cggccgccgg cgccgaaggg tgccagcgcc tcccccgccg ccagcgccac cagcaggcgg
    10561 caggccgcgg ccagggtggt caccggcagc gggctcaccg gcccctcgcc ggcgcgcagc
    10621 agctcggcca ccaggcagac cgccgccagg gccgcgaggc cgaagccgcc gaggatgcgc
    10681 cagccggcgt cctccaggcg gcgccggcca acgaccgcca ccaggtcggc ggcgaggagc
    10741 gccacggcga ggatcgccag cgcccgtccc cagggggagg aggtgatcgg caccagcccc
    10801 gccgccacgg tggcgtccgg gtgggtgtgc gcccaggaga gcgcggccag cacccccccg
    10861 gcatagagcg gcaggatcgt gtagccccag aagcgccggg cctcctcggg acgccccggg
    10921 agccggctcg ccagccgcca tggcgccacc gcctgcgccc cccaggcggc gagcagcgcc
    10981 gccatcacca gaccgtcgag cagggtgttc atggccgacc ggtcagagca gatcccagcc
    11041 gcgccccaac acccgctcca gaggttccgg ggcgacgcgg gcgaggagct cgcgcaccgg
    11101 cgcgccgtcg ggcggcaccg gcaggtccgg cgcgatctcg gccagcggct cgagcacgaa
    11161 gcggcgctcg cgcagacggg gatggggcag ggtcatctcg gcagagtccg aaaggaggcc
    11221 atcgtagagc aggaggtcga tgtcgagggg acggggtccg aagcgcgggc cccggcgacg
    11281 gccggcagcc cgctccagcg ccttggcgag ggcgaggacc gcctcgggcg gcagcgccgt
    11341 gcgcgccagg gcggcggtgt tcaggtagtc gggctggggg atcggggaca ccgcccggct
    11401 gcggtagagg ggggcggcca cgagaggccc aaggctcgcc cggagctggc ggagggcgag
    11461 gcgcagggtc tgctccaccg ccccctcgtt gccacccagc gccagcgcca ccgtcaccgg
    11521 ggtcatcggg gtccctgggt gacgccgccg gtcactcggc ctcgtcgaac tcctcctcgt
    11581 cgtcgtcctc gtcgaggtcg gcgtcgatat cggccgcctc gatgtcgtcg tccggaatct
    11641 ccaccggggc gtcgtcctcg tcaccgtcga gggggcgcgg cgcgacctcg gccttgcgct
    11701 tgcggcgcga ggcctggctg acggccgggg tgtccggcgt cgcgtccttc tggttggctc
    11761 cgcatttggg gcagaggggc tccgcctttc cgaggtcgta gaacttcgtc ccacagttga
    11821 aacactcgta cttggctccc agcttcagct caggcatcgt cgctctctcc gtcaattccc
    11881 cggtcggacc ggctcagagg gcggtgaaac tagcacccgc tgaaacgggt gtcaaccaaa
    11941 gggacggaaa cggcgctaaa ccgggtcgcc gtggacgaag accttggagc gcggcagctc
    12001 gtcctcgatc gcacgcagca cccggaccat gaggtcgtgc gcctcctcga acgatttgcc
    12061 gcgcgggatc tccaggtgga gctcgatgaa gcgctggccg ccgccgcggc gggtcttcaa
    12121 gtcgtgcagc cccaccacgt ccggggcgaa ggaggcgacc acctcttcga tgcgccgcag
    12181 ctcctccgcc ggcagcgcgc ggtccgagag gacgccgaca gactccagca ccagcgcgcc
    12241 gcccgacctg aagacgagga cggccacgcc catggcgacc agcgggtcgg ccggcgccca
    12301 gccggtccag cggacgatga ccagggcggc gacggcggcg ccgttgaccc agaggtcggt
    12361 ctggtagtgc aggctgtcgg ccgcgagggc ggggctctcg gtccgccgcg cctcgcggcg
    12421 cagcaggcgc gcgacgccca ggctcaccag cagggagagg accagcaccg ccgcccccag
    12481 cccggtctgc cgtggcgcct cgcccgagga cagccgcccg acgccggcga cgaccaggct
    12541 gacggcggcg gtcatcagga acagcccctg cccgagcgcg gcgaggttct cgaacttgcc
    12601 gtggccgtag gcgtggtcgg cgtcgggcgg cgcggcgctg cgccgcagca ccacgtagtt
    12661 ggcggcggag atgaagacgt cgatcagcga gtcgagggcc gccgagccga gggcgaggga
    12721 gccggtgatc caggcgcccg cccccttgag gagcacgagc aaggcggcgg cggcgaccgc
    12781 caccaggggg gcgcgggggg gctgcaagga cggctggttc cgggagctat ccggggtggc
    12841 gggcgaggag ggccttggca tccgcgatcg ccttctcctc ttcgacgacg cggtccgcgg
    12901 ccggctgccg cgcggccagc cggcgcagga tcgtccgcgc ctcggcgctc ttgtccggct
    12961 ggtgctcgag cagggcctcg gcgaggaaga gctggttgta ggggtcttcg gggaagagcg
    13021 ccagggcgcg gcgcagctcg gacaccgcct tgccgcggtc gacccagccg gtcaccagcg
    13081 gcaccttggg ggcgagcgta tggagacgcc ccaggaggcg gtggccgccg gcggtctcgt
    13141 agcgctcgtc gagggcgacg acgatctcgc cgtagcgccg gatcttgtcc cctaccccct
    13201 gccgcgccgc ggcgatccgg ccgaaggagt ctccccagag cccccagtgg atcgcggcat
    13261 agaggaagac gggggtggcg tcccgcgtgc cggcgagcgc cttggccgcc tgctgcgggg
    13321 agagggcgtc gagcttggcg cggccgccgg cctggcgggc ggcgaggtcg atggccttct
    13381 cggcgacgct cttgccgcgg tcgaaggccg cctggcgctc ctccttggta cgggccacgt
    13441 gctcgccctg gaaatggagg gcccgcatca gcttccactg cgcctccagg ttgcccggct
    13501 cggcgcggac cgcccgctcg taggcggcga cggcctcgcc ggcggggccg gcggcggcgc
    13561 gggcgccccg atgcccctcg gcgcggcggg cccaggcggc atcccccgcc gggatctggg
    13621 ctgcgagggg ggcgcccgcc acgaccaggg cgacgagcac ggcggccggc aggaggaggc
    13681 gggggagggc cggggtcttc cacatggagg ggattttgtc acggatcgga cgggcgctat
    13741 catctctatc cctatggcag accgatcgga cagcaagccc aggctccccc tggtcccgct
    13801 cccggacgtc gtccatttcc cgcgcacggc tctcaagatc cgggtggcgg acccgcgcta
    13861 cctccgcctg gtgcgcgacc tgctggagca ggacgaggag gtgcgctggg cgggcgtggt
    13921 gctgctcaag cccggctggg tgcgcgacgc cgccggccgg ccggagatct tccccggcgg
    13981 caccgcgggc cggctggtgg aggccgagcc ccaggccgac ggcaccaccc agatcctgct
    14041 gctgggcgat ttccgcttcg agctcgaggc cgagctcccc ggcgaccgcc cctaccgcag
    14101 cgccctggtg cggccggtgc aggagccctg gctcaacgag caggaccccg gcatcctggc
    14161 ggtccggcgg tcgctggtgg agctggccgg cgccctcgcc accgagctcg gggagaggtt
    14221 cccgttcgac gccggagaga tcgaggacct cgccgagcgc tccgatttcg aggagctgat
    14281 caaccggctc gccgccggct tcgacctgcc gccgctgcgc aagctgcagc tcctcagcga
    14341 agcgctgccg gagcgcgggg agtcgctgct gtcgatcctc cgccaccgcc agcaggtgct
    14401 ggactcgctg cgcccctacc gccacctggc gggcggctcc gagcacaact gagcgcgccc
    14461 cgtgctgtcg atccgcgatc cggtgcacgg cttcatccgt gccgacccgc tggaggcggc
    14521 gctgatcaac agccgtccgg tccagcgcct gcgcttcatc catcagctcg gcttcacctt
    14581 cctcgtcttc cccggcgccg agcacagccg cttcagccac gtgctgggcg ccatgcacct
    14641 ggccggccgg gtctacgacg cgctctgctc gcgggccgag ggcctgctgc cgccggggac
    14701 cgcgagccgc gagcgccggc tggtgcgcgc cgcggcgctg gtccacgacc tcggccacgc
    14761 gccgttcagc cactcggccg aggggctgtt cgaggacggc atcgaccatg aggagatgac
    14821 ccggcgcctg ctgcgcgcgc cggagatcga ggaggtcttc gcccgccatg gcgccgggat
    14881 caccaccggc gacgtggcgc gcctgctggc cggcggcggc gacccggccg agcggctgct
    14941 ggcgcagatc gtctccggcg agctcgacat cgacaagatg gactacctgc tgcgcgacag
    15001 cctgtactgc ggcgtccgct acggcagcta cgacctcgac cggctgctcg acaccatgct
    15061 gccgatcgcc gaccccgaca ccggcgactg gggggtcggc gtcgacgagg ggggcgtcca
    15121 cgcgctggaa gcgctggtga tggcccgcta ctacatgttc acccaggtct acttcaacgt
    15181 caccggcaag gcgctggagc tgcacctcaa cgagtggctg ctcgagagcg gcatccgctg
    15241 gccggcggag cccgagctct tcctggagca ggacgacgtc tcggtctggg cggcgatgcg
    15301 ggcctcgcgc agcccgcatg cccgggcggt ggtggcgcgg gagcacttcc cggtggcgtt
    15361 cgagacccgc gagcacctga cgcgcgagga gaaggagcgc ttcgaatcgc tgctccccgc
    15421 cctgcgcgag cgcttcggcg ccggcaacct cctgctctcc aactccgcca aggacccgca
    15481 ccggctgggg cgccagcggg tcctggtgcg gcgcttcgac ggctccctcg aaccgatgga
    15541 ggaggcgagc cagttcatcc gccacctgtc gcgcatcgac cgctaccggg tgtacacgcc
    15601 gccgcccctg cgggaggcgg tggcggccgg cttccgcgag cgctgggagc cctctgcaag
    15661 ccctgattca aattcttgat cggctcaccc caaaaaactg aaccgccgcc ctctcccctc
    15721 cctgccctcg cgggccaagt cattgagaca ttggaactta actggcagtt gacattctca
    15781 tgacaccggc atgacaattg ccacgcttat tccccagtgt tccaaaccgc tctcctcctg
    15841 ttgaggaggc atggctcgaa aggagtcgca gcatgagagt caaaatcctt ttggcgtgcg
    15901 tagcgtgcct tctcttggca ctgcccatgc tcgcccaagg tgtccccacc ggcagcgtgt
    15961 ccggccgggt gacttcggcg gaagggacgg cgctgccggg ggtgacggtg accgtcactt
    16021 cgccccagtt gcagggcacc cgctccgcca cgaccagcgc ccagggtgac tacaacctgc
    16081 cgttgctgcc gcccggcgac tatcaggtca actacgagct ggctggcttc ctgacggccg
    16141 agcactcggt caaggtcgcc ggcggcctgc aggcgcggat cgacgtcgag ctggcgccga
    16201 actcggtcag cgaggagatc gtcgtcaccg gcacctacga gaccatctcg accggttcgc
    16261 aggacgccac cacctacgag aaggagttca tcgagcagct cgcgatcgac cgtgacatcc
    16321 gcaacaccac gctgctgacg ccgggcgtct ccgccacggg cccgaccaac gccatcacga
    16381 tctcgggcgc cttgtcctat gagaacctgt tcctggtcaa cggcgtcgcg gtcaacgaga
    16441 acctccgcgg ccagccgtac aacctgttca tcgaggacgc catcgacgag accaccatct
    16501 cgacctcggg catctccgcc gagtacggcc gcttcggcgg cggcgtggtc aataccatca
    16561 cccgctccgg cggcaacgag ctgcacggct cgttgcgtac cgtcttcctc aacgacgact
    16621 ggatcggccg cacgccggtt tcgcccgagc ggctcgacga catcaaccag cgttatgagg
    16681 cgaccctcgg cggctgggtc ctcaaggacc gcgtctggta cttcctggcc ggccgcgact
    16741 acgagctctc cggcagcagc gccacggcgc tgacgcagat cccgtacgag accggcgagt
    16801 ccgagacccg cctcgagggc aagctgacgg tctccccctt ccaggggcac cgcctgatcg
    16861 gctcctacat ggagatcgag cgcgaggaga ccaacgcccg cttccagtcg atcctcgaca
    16921 tcgagagcct caagagccgc accctgccga acgagctccg ggcgctcaac tacaccggcg
    16981 tcctgacgga gagcttcttc gtcgaggcgc agtactccga gcgcttcttt gccttcgagg
    17041 gtgacggctc caagttcacc gaccgcatcc gcggcacgct gctcatcgac gacgagctcc
    17101 accgctggaa cagcccgacc ttctgcggca tctgccggcc cgaggagcgc aacaacgaga
    17161 acatctacgc caaggcgtcc tggttcgcct cgaccgagag cctgggctcc cacgacatcg
    17221 ccttcggcta cgacagcttc gacgacatcc gcgtcgccga caaccaccag tcgggcagcg
    17281 acttccgcgt caacctgagc gacacctgga tcatcggcac caacctctac ccgcagctcc
    17341 tgaacgacaa cacgacgatc atcacctgga acccgatcct ccagggcagc cggggcacca
    17401 gcttcgtcac caactcgatc ttcgccaacg accgctggcg cctgaacgag aactggagct
    17461 tcaacctcgg cgtgcgctac gaccagaacg acggcaagga cagctccggc aagaaggtgg
    17521 ccgacgacag ccggatcagc ccgcgcctcg gcctcacgtt cgatcccaag ggcgacggcg
    17581 actgggtgtt caacgccagc tacggccagt atgtgtcggc cctcgccaac acccagggtg
    17641 acgccacctc ggcggccggc aacccggcca acatcacctg gttctaccgc ggcccgaaca
    17701 tcaacccgga cggcaccccc gccgaccagc tgatctccac cgaagaggcg ctgcggcgca
    17761 tgttcgagtg gttcgaccag cagggaggca cgaactacgc ggtccaccgt ccccgctccc
    17821 tcaccatccc cggcgagacc tcgcggatcg tcgacgggct caagtcgccg gtcaccgacg
    17881 agatcgcgct gggcgtcagc aagcggctcg gcaaccgggg catcgcccgc gccgagtacg
    17941 tacaccgcga gggtcacgac ttctacgtcg accgccgcga cctcaccacc ggtcaggtga
    18001 cgctgtccaa cggcagccgc gccgacctct cggtcatcga gaacgaggac gacctgctcg
    18061 agcggaccta cgacggcctc cacacccagt tccagtaccg ggtgggcgac cgcctgagcc
    18121 tgggcggcaa ttacaccctc tccttcctca aggggaactg gaccggcgag aactcggcct
    18181 ccggcccggt ccgctcgacc atcctccagt accccgagta catcgacgtc gactggttcg
    18241 cccccgaggg ctacctcagt gccgaccagc gccaccgtgc ccgcgtctgg ggcctcttcg
    18301 acctcttcaa gaccgaccat caccggctga acctcagcgt gctgcagctc tacacgtccg
    18361 gcacccccta cggcgccctc ggcgccgtgc ggtcgctgaa ctacaacgac aaccccggct
    18421 atgccatcga gccgacgagc gtcgcctact ttttcaccga ccgtgacgag ttcacgaccg
    18481 aagacatcac cagcaccgac ctcgcgctca actactcgtt cacctggaac gccttcaaca
    18541 agtcgatgga ggtgttcatc cagcccgagg tgaccaacgt gttcaacgag gacggcgcga
    18601 tcctcgtcga ccagaccgtg cgcgacaaca cgaccaccac gaccatcccg tccttcaacc
    18661 ccttcaccga gaccccggtc gagggtgtcc actggcagcg cggcgcgaac ttcggcaagg
    18721 cggtcaacga gaccggcttc cagacgcccc ggaccttccg gttctccgtg ggcttccgct
    18781 tctaaccccg cgttccgtcc tgtcgtagag gagccccggt ccacggaccg gggctttttc
    18841 ttttcaggcg cttcggagag gaacggccgc cgccaggcgg gccagggccg tgcgccaacg
    18901 gacgcgatag ctctcccagg cggtgaggcc gagggagagg gggagacgct gaaacccctc
    18961 ctgaagcacg tcgagctcgc aaccggcggg agtggcggcg aggcgcaggg tcagccgggt
    19021 gggagcggtc cagccggcct cgagccgttc gaaagagagg acccagaggg tggcggggat
    19081 cgtccccagg atcctgcccc gctcgcgcac gggctcgccg cggtctcccg tgatccgcag
    19141 aaggagctcc tcttccctgt ccccggcgat ccgcgcctcc tcggcgagcc agaggcagag
    19201 ccgctccggc tccaccagcc agcgccaggc ctcggccggg cccagggcga ggtcctggcg
    19261 ccggcgtagc tgcacgcccg ggatgtcccc ctggcggatc gcctcgcgcg cctcccgcat
    19321 cggtgcctag tccaccaggt tgcggagggc cggctccagc tccgggtagc ggaaggggaa
    19381 gcccaggtcg aggagacgcc gggggacggc ccgctggccc tggagcagca tgccggccat
    19441 ctcccccagg gcgacctgga agaccgcgcg cggcaccccg agcccgaggg cctggagcgg
    19501 gttcggccgc cgtaccaccc ggctgaggac gtccccgagt tgccggttgg tgaccgggtt
    19561 gggagcgctg aggttgaacg ggccgctggc ctcccggcgc tcgaggagga agcggatggc
    19621 cgccacctcg tcggccatgt ggatccaggg gaaccactgg cggccgctgc ccaggggggc
    19681 gccggcgccg agccggaagg ggagcatcat gcgcggcagg gcgccgtcct tcaacgagag
    19741 cacgatgccg gtgcgcagca atgcgcgccg cacccccagg gcctccacct cggtggtcga
    19801 ggcctcccac tcccggcaga cgtcggccag gaagtcgttc cccgatggat ggtcctcggt
    19861 caccacctcg tcgccgcagg ggccgtagta gcccacggcg gatccctgca ggaggaccgc
    19921 gggcgctcct gccgaggaag aggcggcctg gcggacggct tcgagcaccg ccttccccga
    19981 ctgcacccgg ctgtcgcgga tgcggcgctt cttctccgcc gtccagcggc cggaggcgat
    20041 gctctccccc gccagatgga cgatggcggt gccggcgtcg aggagcttcg accacccctg
    20101 gccgctcctg ccatcccact ccgccgcccg cacgcccggg ggcagtgccc cgacgcggct
    20161 caggttgcgc gagaggacga cggcgtcatg gcccgctccc cccagctccc tggcgagcgc
    20221 gctgccgatg agccctgatc cacctgtgat gacgacacgc atggctcctc cctcctctgc
    20281 ccctccgcaa aggatggtac atcagagggg gaagggggtc agactgcggc gagctcttct
    20341 tcgaggagct ggcgctggtg gaggtcggcg tacaggccgc cgtgggcgag gagctgcgcg
    20401 tgggtgccgc gctcggcgat gcggccctgg tccagcacca ggatgaggtc cgcgtccttg
    20461 accgtggaga tgcggtgcga gaccaggaag accgtgcgcc cctcgaaaac ggtgcgcagg
    20521 ttccgcagga tctgctcctc ggtgtgggtg tccaccgccg acaggcagtc gtcgaggagc
    20581 aggatgcgcg ggcggcgcag cagggcgcgg gcgagggcga cgcgctgctt ctggccgccg
    20641 gagagggtga tgccgcgctc gccgacgatg gtgtcgaggc cgttggggaa ctgcgacagg
    20701 tcggagccga ggccggccag gcgcgccgcc tccaggatct cgtcgtcggt ggcgccgggg
    20761 cggccgaagc cgatgttctc gcggatcgag gccgagaaca ggaagctctc ctgcg
//
LOCUS       DASPJI010000619         4811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_336856, whole genome shotgun sequence.
ACCESSION   DASPJI010000619 DASPJI010000000
VERSION     DASPJI010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336856"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(802..987)
                     /locus_tag="VEL74_17330"
     CDS             complement(802..987)
                     /locus_tag="VEL74_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64344.1"
                     /translation="MKKQRKLPKLTLQKETLRSLAGGFERNRPVNSDIIYTVYSTDVG
                     CRPVTDTCRTACQVCWA"
     gene            1462..1842
                     /locus_tag="VEL74_17335"
     CDS             1462..1842
                     /locus_tag="VEL74_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64345.1"
                     /translation="MASLSAPWNETGLSEEPAIQVLQTLRWDYVPPEVLDAERGSPRE
                     TVLERRLARSLRRLNPWLSDDNVHKVLRTGASPRAWTGCCPIRGRRRTWPTSGAWARS
                     GRRRGRASGTRPSTSPTARPRCGG"
     gene            1903..2124
                     /locus_tag="VEL74_17340"
     CDS             1903..2124
                     /locus_tag="VEL74_17340"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYG64346.1"
                     /translation="MRRQLLVWYRRHAEERIPERVEIWTQQTGLAHELAHLLHPGHTR
                     DFWAALGRILPDYEERREALRRLGDRLVW"
     gene            complement(2168..2407)
                     /locus_tag="VEL74_17345"
     CDS             complement(2168..2407)
                     /locus_tag="VEL74_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64347.1"
                     /translation="MKRGLLLSVLVAALGLAGWVTGTPRAEASGFCDASEGLSCSPAG
                     DMFNCFWSTGERGFCFCEPVAGQPGYYWVCGTGKP"
     gene            2594..3610
                     /locus_tag="VEL74_17350"
     CDS             2594..3610
                     /locus_tag="VEL74_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiG"
                     /protein_id="HYG64348.1"
                     /translation="MPLFDTYMIVDWSASSSPGVGRDTVWIGLVERGARRSRAQNPPT
                     RVQGTAEIVRILREQKERGRRVLVGFDFAFGYPQGFAKALGLKGKAKPWRRIWDAIGE
                     RFQDKPDNSNNRFDVAAELNQQVGTPEMPGPFWGRPHHRADLTSISMKSLTYPFPAGK
                     GAALERFRRTEEILREAGWNNIQEVWKLYTAGSVGGQTITGVPVVRALRDSPDFAGHI
                     QVWPFETGFTDRPGGASGGGGEGRRPYILLAEIWHGFLRPLDMLEGEHRDERQVLGLA
                     RLCEKLDNAGRLGRLFDTPAGMSPEQARICVEEEGAILGADPEFLAERPPKRKAPLPY
                     PFFS"
     gene            complement(3607..4365)
                     /locus_tag="VEL74_17355"
     CDS             complement(3607..4365)
                     /locus_tag="VEL74_17355"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HYG64349.1"
                     /translation="MPDTPEMTGTETGAPQQSTTHQPATGTPTASATAGQTSASPQGG
                     YPQTPQTGQPGQPAMSHRGRGPMTPWDMAMSGNPFGMMRQFADEMDRLFESFGFPSFG
                     RQWPGRNVVSNLEQQQDVQGVSSAGGPGGAMSRPQGGGAFWAPQIDVSRRGNELVVCA
                     DLPGMNREDVNVEVRDGQLILQGERRWQQEQGGEAEGYYRSERSYGRFFRTVPLPDGV
                     NPEQAQATFKDGVLEVKMPLPEQHEQRGRKIEIR"
     gene            complement(4574..>4811)
                     /locus_tag="VEL74_17360"
     CDS             complement(4574..>4811)
                     /locus_tag="VEL74_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64350.1"
                     /translation="VRPPDRMPADARALARFHLIDLRERLTRRLTPPASFDAYTQAHL
                     MDARERIDMALKAEYQIDPSAAAATPIGGAPARD"
BASE COUNT          690 a         1753 c         1632 g          736 t
ORIGIN      
        1 gagcctcggc ttgcacgccc attcgaagct gtggcagtca caggtgtaaa acgccgtgat
       61 gccagcgccc accaccccat tcaggtcggc tcccaccagc ctcctcaccg tctccttcgc
      121 cagcaccagc ttcttcatcg atttcttcat gctgcctcct tgtcctggtc gatcccgggt
      181 tccgcacccg ggcgggcgca ttttaggcct ctctcgatcc cggatccagg cttgtcttcc
      241 cccctgccat ttctcgaccg ccgtacccgg cggcgcggcc tccatccccg cggagatgac
      301 ggtcctaccc agggtaggaa ggctctaccc gtgggtacgg ccttcctacc cggggtacgg
      361 agaccctccg cgcgggtacg aaggccatac ccggcgaatg gagaccctcc ccgcggcgac
      421 ggacacgcta cccagggtag gaaggacgtc tctccgggta cggaggccat ctccgcgggg
      481 atgacggccg ttctcctggg gcaatggctg ccccgggagc gacgagcttc tcctacgcct
      541 ccgcggcgac gaggccccct tgcttatcgt ccgaggagcg cggtgcgggt ctcttccccc
      601 gctccctctc gcccaaccgc ccggagatgg cggcttcatc ccccctgcgc cctcaaagct
      661 gacgcgcgga ggcccctcgt cccctcggga ggtgcgaccg gaacagaggg aggacgggat
      721 tcttcggggc gtcccgatgt gtgacagggg ctctggcggg atggaaaggc gggggcgggc
      781 agcccgcccc cggagagagg ctcaggccca gcagacctgg caggccgttc ggcaggtgtc
      841 cgtcaccggg cggcacccga cgtctgtgct gtaaaccgta tagatgatgt cggagttcac
      901 aggcctgttc cgctcgaacc cgccggcgag gctcctcagg gtctctttct gaagggtgag
      961 cttcggcagc ttccgctgtt tcttcaagat ggacctcctt tcattcgtcg cgccgatgat
     1021 gcgcggggga gattttactc atcccggccg ggatggcaac gggccgcccc cacctcgatc
     1081 ctccccctgc tccaacccgc cccctcccgg agctgccacg ccctgtcgcg tcgggtactc
     1141 gccccacggg gccacgtgca ggccccacag ggtgacgggc tcgccccata gggcaacggg
     1201 ctcgccccat gggtcaacgt gctcgcccct caggtcgtcg tgctcgccct acagggccac
     1261 gtgcgcgccc cacgggtcaa cgtgctcgcc cctcagggca acgggctcgc tctagagggc
     1321 aacgggctca cccctcaggt cgacatgctc gcccctcagg gcaacgggct cgcccctcag
     1381 ggcagatcgc gtccgccgcc ccggcgcgcg tggcgtccaa ggggccgctc cgtgtagagt
     1441 tatcactcga aacgtacagc catggcttcc ctgagcgccc cctggaacga gaccggcctg
     1501 tccgaagagc cggcgatcca agtcctccag accctaagat gggactacgt ccccccggag
     1561 gttctggacg cggagcgcgg cagcccgcgg gagacggtcc tcgagcgccg cctcgcccgg
     1621 tcgttgcggc ggctcaaccc gtggctgtcc gacgacaacg tccacaaggt gctgcggacc
     1681 ggcgcttcgc cgagagcatg gaccggctgc tgcccgatcc gggggcggcg acgtacctgg
     1741 ccgacctccg gcgcctgggc aagatccggc agacggcgcg gacgcgcttc cgggaccagg
     1801 ccctcgacct ctccgactgc gcgcccaagg tgcggcggct gatcgaggag gcgatcgcgg
     1861 ccggccgagg gggacctgcc gccggcggag cggtcggccc gggtgcgccg gcagctcctg
     1921 gtctggtacc ggcggcacgc ggaggagcgg atcccggagc gggtggagat ctggacgcaa
     1981 cagacgggcc tcgcccacga gttggcccac ctcctccacc ccggccacac ccgcgacttc
     2041 tgggccgccc tcggccgcat cctgccggac tacgaagagc gccgcgaggc cctgcgtcgc
     2101 ctgggcgacc ggctggtctg gtagcagggg gaggaggggc ggctccccgc ccctcctccc
     2161 tccatcccta cggcttgccg gtgccgcaca cccagtagta gccgggctgg ccggcgaccg
     2221 gctcgcagaa gcagaagccc ctctcccccg tcgaccagaa gcagttgaac atgtccccgg
     2281 ccgggctgca ggacagcccc tccgaggcgt cgcagaagcc ggacgcctcc gcccgcggcg
     2341 ttcccgtgac ccagccggcg agccccagag ccgcgaccaa caccgacagc agcaaccctc
     2401 gtttcatcga agacctcctt gggatttgcg ggggatagcc ccctgatcct gaagctgccg
     2461 ggtagcgtca gagcgggaca gatgcgcaca cgacgtccgc ctcgtgtaaa gtggggcgcg
     2521 gtttccggat ggaccgcctt ggggccgttc ggccatacag gcgggcaggt gatatggtga
     2581 gggggaggaa aaaatgcccc tgttcgacac ttacatgatc gtggactgga gcgccagctc
     2641 ttcccctggc gttggcagag ataccgtttg gatcggcctc gtggagcgcg gcgcccggcg
     2701 ctccagggcc cagaaccctc cgacccgcgt ccagggcacc gccgagatcg tccgcatcct
     2761 gcgcgagcag aaggagcgtg gccggcgggt cctggtgggc ttcgacttcg ccttcggcta
     2821 tccccaggga ttcgccaagg ccctgggcct gaaaggcaag gccaagccct ggcgccgcat
     2881 ctgggacgcc atcggcgagc gcttccagga caagcccgac aactccaaca accgcttcga
     2941 cgtcgcggcg gagctcaacc agcaggtcgg cacccccgag atgcccggcc ccttctgggg
     3001 ccgcccccac caccgcgccg acctgaccag catctccatg aagagcctca cctacccctt
     3061 ccccgcgggc aagggggcgg cgctcgagcg cttcagaagg acggaggaga tcctgcgcga
     3121 ggcgggctgg aacaacatcc aggaggtctg gaagctctac accgcgggat cggtcggcgg
     3181 ccagacgatc accggcgtgc cggtggtgcg ggcgctgcgc gactcccccg acttcgccgg
     3241 ccacatccag gtctggccct tcgagaccgg cttcaccgac cggcccggcg gcgctagcgg
     3301 cggcggcggc gagggccggc ggccgtacat cctcctcgcc gagatctggc acggcttcct
     3361 gcggccgctg gacatgctcg agggggagca ccgggacgag cggcaggtcc tcggcctggc
     3421 ccggctgtgc gagaaactcg acaacgccgg ccggctcggg cggctcttcg acacgccggc
     3481 ggggatgtcg ccggagcagg cgcggatctg tgtcgaggag gagggggcca tcctgggtgc
     3541 ggacccggag ttcctggcgg aacggccgcc caagcggaag gccccccttc cctatccgtt
     3601 cttcagctag cggatctcga ttttgcgtcc ccgctgctcg tgctgctcgg gcagcggcat
     3661 cttgacctcc aggacgccgt ccttgaaggt ggcctgcgcc tgctcggggt tgaccccgtc
     3721 gggcagcggc accgtccgga agaaccggcc atagctccgc tcgctccggt aatagccctc
     3781 cgcctcgcca ccctgctcct gctgccagcg gcgctccccc tggaggatga gctggccgtc
     3841 ccgtacctcg acgttgacgt cctcccggtt catgccgggg aggtcggcgc agaccaccag
     3901 ctcgttgccg cgccggctga cgtcgatctg cggcgcccag aacgcgccgc cgccctgcgg
     3961 ccggctcatc gcgccgcccg ggccgccggc cgagctcacc ccctggacgt cctgctgctg
     4021 ctccaggttg gagaccacgt tccggcccgg ccactggcgg ccgaaggacg ggaagccgaa
     4081 gctctcgaac aggcggtcca tctcgtccgc gaactggcgc atcatgccga acgggttgcc
     4141 gctcatggcc atgtcccagg gggtcatggg gccgcggccg cggtggctca tggccggctg
     4201 cccgggctgg cccgtctgcg gcgtctgcgg atagccgccc tgcggggagg cggacgtctg
     4261 gcccgctgtc gcgctggccg tgggggtgcc ggtcgccggc tggtgggtcg tggactgctg
     4321 aggggccccg gtctcggtgc ctgtcatctc gggagtgtcg ggcatgctgt cagctccttt
     4381 ctggttctcg ggccaaggag caggaaactg caaaaagctc tccagaagct ccatgcgccg
     4441 cccggcagcg cgcggacccc tgccaagcgg agacggatgg cacggatccc tccgtcttcg
     4501 ctctctggag gagacgccat gaagtcgaga ctcgtcctct gcctcgccct cgtccacatc
     4561 ccgccggagg ggctcagtcc cgcgccggcg cgccgccgat gggggtggcg gccgccgcgg
     4621 acggatcgat ctggtactcg gccttgagcg ccatgtcgat ccgctcccgg gcgtccatga
     4681 ggtgcgcttg ggtataggcg tcgaagctgg cgggcggggt gagccgccgg gtgagccgct
     4741 cccgcaggtc gatcaggtgg aaacgggcca gcgcccgggc gtcggccggc atgcggtccg
     4801 gcgggcggac c
//
LOCUS       DASPJI010000620         3789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_336918, whole genome shotgun sequence.
ACCESSION   DASPJI010000620 DASPJI010000000
VERSION     DASPJI010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336918"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="VEL74_17365"
     CDS             <1..1092
                     /locus_tag="VEL74_17365"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYG64351.1"
                     /translation="DALNNLRRPERQRTAFLVGPERLELRDVPVPAPGPGELLVRIGA
                     ATTCGTDLKVFRRGGHPRMLKAPTPFGHEMAGTIVEVGDAGDSAEPWKAGDRVVVANS
                     APCGVCEWCRRRRENLCSDLHYLNGAFSQYILVPRRFAAVSTYRCPEGLPFEVAALAE
                     PLACVLHGLEISGIEGPCEIVIYGGGPIGLLFADVLAHAPEYSADGGHHIVLADPNPE
                     RLEVARRLGADEGVQVGREGGEAAHVRAHARGGDGFHLAIEATGSPAAWQDALASVRP
                     GGVVQLFGGCPPGTTVPLDTHRLHYSEITVKSAYHHRPATFARALDLLASGALHPEAL
                     LSAERPLAELEDALRSMRRREALKVVIRP"
     gene            1120..3054
                     /locus_tag="VEL74_17370"
     CDS             1120..3054
                     /locus_tag="VEL74_17370"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: protein motif:HMM:NF008559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HYG64352.1"
                     /translation="MIPSRVTALWLSALTLGASLGAAAPHPLDPLSAPEIGAVRRAIL
                     ADPRFPVDGLFAEVSLREPAKEAVLAARPVPREAFAILYSRKQDKTWEATVDVTDPAA
                     PRIRAWEHVPGIQPVYMHDDEAVFYCHLNESEEWKAALQKRKIHPQDVKTDFWVYGWS
                     DQARPRSFRAVPYFRGEGDNYYSRPIEGLEAVVDLVHGKVEVIDTWDGEPVPPESPEF
                     DEERIREAHGLRAPLKPLQITQPDGPEFTTDGQEVRWQNWRFRWQMHPRSGLVLHQVR
                     YVTKENGKEKERSILYRGSLSEMAVPYGDPRASWAWRSAFDLGDYDVGHLAAALRKGL
                     DVPENAQLFGALFAGNDGRPWGLDDVVAIYERDGGLLWRHADNARRGRELVLFSVSTV
                     GNYDYGISWIFKQDGSMEVETALTGMMLAKAVKDGHHDPYSHQVAPNVAAVHHQHVLS
                     FRLDMDVDGPANSVYSMNSFSKDPQPPYYNTFVMEETAFEREREACQTQNMATARTWR
                     VGHRAAGNGGGGHAGETGYILVPGSNSLPLLDERAPNRQRGRFMDCHLWVTRYAPREL
                     YAAGDYPNQSTGSKGLPEWTQANRKIRDEDVVLWYTLNVTHVPRPEEWPVMTTSRYGF
                     KLVPAGFFPRNPALDVPLPQ"
     gene            3067..>3789
                     /locus_tag="VEL74_17375"
     CDS             3067..>3789
                     /locus_tag="VEL74_17375"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYG64353.1"
                     /translation="MPRPVTVLSLLLLAPAVSAGAAEPFADRAAEWGLAFTYTTGDTG
                     QFYYPEIIGGGAALFDYDGDGDLDVFLVQGRVLEPGKEGADPAAARGGRLFRNDLIAG
                     GTRNPAPRFVDVTEASGIRAHGYGMGVAAGDYDNDGRIDLYLNNFGSNQLWRNQGDGT
                     FKDVTKAAGADDPRMSLSASFADFDRDGWLDLFIANYVDFSVESNVVCYAKSSRRDYC
                     GPSAFPPTPDRLLRNRRDGTFED"
BASE COUNT          576 a         1388 c         1285 g          540 t
ORIGIN      
        1 gacgccctga ataatctccg gcggcctgaa aggcaaagga ccgcgttcct ggtggggccg
       61 gagcggctgg agctgcggga cgtgcctgtc ccggcgcccg gccccggcga gctgctggtg
      121 cggatcggcg ccgccaccac ctgcggcacc gacctcaagg tgttccggcg cggcggccat
      181 ccgcgcatgc tcaaggcgcc cacccccttc ggccacgaga tggcgggcac gatcgtggag
      241 gtgggtgacg ccggcgacag tgcggagccc tggaaggccg gcgaccgggt ggtggtggcc
      301 aattcggcac cgtgcggcgt ctgcgagtgg tgccggcggc ggcgcgagaa tctctgctcc
      361 gacctccact atctgaacgg cgccttctcc cagtacatcc tggtcccccg ccgcttcgcc
      421 gcggtgagca cctaccggtg ccccgagggc ctccccttcg aggtggcggc gctcgccgag
      481 ccgctggcct gcgtgctcca cggcctggag atctccggga tcgagggacc gtgcgagatc
      541 gtgatctacg gcggcggccc catcggcctc ctcttcgccg acgtgctggc gcacgcgccg
      601 gagtactcag ccgacggcgg gcaccacatc gtgctcgccg acccgaaccc cgagcgcctg
      661 gaggtggccc gccggctggg ggcggacgaa ggcgtgcagg tcgggaggga ggggggcgag
      721 gcggcgcacg tccgcgccca cgcccggggg ggagacggct tccacctggc catcgaggcg
      781 acgggctccc ccgccgcctg gcaggacgcc ttggcctcgg tgcgcccggg cggcgtcgtc
      841 cagctcttcg gcggctgccc gccggggacg accgtgccgc tggacaccca tcgtctccat
      901 tactccgaga tcacggtcaa gagcgcctac catcaccgcc cggccacctt cgcgcgcgcc
      961 ctggacctcc tcgcctccgg cgccctccac cccgaggccc tcctctccgc cgagcgcccg
     1021 ctcgcggagc tggaggacgc cctgcgcagc atgcggcggc gggaggcgct caaggtggtg
     1081 atccggccat gatgtctatt ctttgatcag gagagagcca tgatcccatc ccgcgtgacg
     1141 gccctgtggc tgtcggccct caccctgggg gcgagcctcg gagccgcggc cccgcatccc
     1201 ctcgacccgc tgagcgcgcc ggagatcgga gcggtccgcc gggcgatcct cgcggacccg
     1261 cgcttccccg tcgacggcct gttcgccgag gtctcgctga gggagcccgc gaaggaagcg
     1321 gtcctggctg ccaggcccgt gccccgggag gccttcgcca tcctctactc ccgcaagcag
     1381 gacaagacct gggaggcgac ggtcgacgtc accgacccgg ccgcgccccg gatccgcgcc
     1441 tgggagcacg tgcccggcat ccagcccgtc tacatgcacg acgacgaggc ggtcttctat
     1501 tgccacctca acgagagcga ggaatggaag gcggcccttc agaaacgcaa gatccaccct
     1561 caggatgtga agaccgattt ctgggtctat ggctggagcg atcaggcccg gccgcggagc
     1621 ttccgggccg tcccctattt ccggggggag ggcgacaatt attacagccg cccgatcgaa
     1681 gggctggagg cggtcgtcga cctggtccac ggcaaggtcg aggtgatcga cacctgggac
     1741 ggcgagcccg tgccgccgga gagcccggaa ttcgacgagg agcgcatccg cgaggcgcat
     1801 ggcctgcgcg ccccgctcaa gccgctccag atcacccagc cggacggccc tgagttcacc
     1861 accgacggcc aggaggtgcg ctggcagaat tggcgcttcc gttggcagat gcacccgcgc
     1921 tcggggttgg tcctccatca ggtccgctac gtgacgaagg agaacggaaa ggagaaggag
     1981 cgctccatcc tctatcgcgg ctcgctctcc gagatggccg tgccctacgg cgacccgaga
     2041 gcgagctggg cctggcgctc ggccttcgac ctcggggact acgacgtcgg ccacctcgcc
     2101 gcggcgttgc gcaagggcct ggacgtcccc gagaacgccc agctcttcgg cgccctcttc
     2161 gccggcaacg acggccggcc ctgggggctc gacgacgtgg tggcgatcta cgagcgggac
     2221 ggcggcctcc tctggcggca cgcggacaac gcgcggcgcg gacgcgagct cgtcctcttc
     2281 tccgtctcga ccgtcggcaa ctacgactac ggcatctcct ggatcttcaa gcaggacggc
     2341 tccatggaag tggagaccgc gctgaccggg atgatgctcg ccaaggcggt caaggacggc
     2401 caccacgacc cctacagcca ccaggtggcg ccgaacgtcg ccgccgtgca ccaccagcac
     2461 gtcctgagct tccggctgga catggacgtg gacggcccgg ccaactcggt ctattcgatg
     2521 aacagctttt cgaaagaccc ccagccgccc tactacaaca ccttcgtgat ggaagagacc
     2581 gccttcgaga gggagcgcga ggcctgccag acccaaaata tggcgaccgc ccgcacctgg
     2641 cgggtcggcc accgggccgc tggcaacggc ggcggcgggc acgccggcga gaccggctac
     2701 atcctggtgc cgggctccaa ttcgctgccg ctcctcgacg agcgggcgcc gaaccgccag
     2761 cggggccgct tcatggactg ccacctctgg gtgacccgct acgcgccgcg cgagctgtac
     2821 gccgccggcg actatccgaa ccagtccacc ggctcgaagg gtctgcctga gtggacccag
     2881 gcgaaccgca agatccgcga cgaggacgtg gtcctctggt acaccctgaa cgtgacgcac
     2941 gtcccgcgcc ccgaggagtg gccggtgatg accacctcgc gctacggctt caagctggtc
     3001 cccgccgggt tctttcccag gaacccggcg ctggacgtgc ccctgccaca gtaaaatggt
     3061 ggggcgatgc cccgtccggt caccgtcctc tccctgctgc tcctggcccc ggctgtgtcc
     3121 gccggggccg cggagccgtt cgccgaccgc gccgccgagt gggggctcgc cttcacctac
     3181 acgaccggcg acaccggcca gttctactac ccggagatca tcggcggcgg cgcggcgctg
     3241 ttcgactatg acggcgacgg cgacctcgac gtcttcctgg tccagggccg ggtgctggag
     3301 cccggcaagg agggggctga tcccgcagcg gcgcgcggcg ggcggctctt ccgcaacgac
     3361 ctgatcgccg gcggcacccg gaacccggcg ccgcggttcg tcgacgtcac cgaggcgagc
     3421 ggcatccggg cccacggcta cggcatgggc gtcgcggccg gcgactacga caacgacggc
     3481 cggatcgacc tctacctgaa caacttcggc tccaaccagc tctggcgcaa tcagggggat
     3541 ggcaccttca aggacgtcac caaggccgcc ggcgccgatg atccgcggat gagcctctcc
     3601 gccagcttcg ccgacttcga ccgcgacggc tggctcgacc tgttcattgc caactacgtc
     3661 gacttctcgg tcgagagcaa cgtcgtctgc tacgcgaaga gcagccggag agactactgt
     3721 ggcccctccg cgttcccccc cactccggac cggctgctgc gcaaccgccg ggacggcacg
     3781 ttcgaggac
//
LOCUS       DASPJI010000621         3528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_672172, whole genome shotgun sequence.
ACCESSION   DASPJI010000621 DASPJI010000000
VERSION     DASPJI010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672172"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VEL74_17380"
     CDS             complement(<1..439)
                     /locus_tag="VEL74_17380"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYG64354.1"
                     /translation="MNNQPRPKFTWIGHATVVCELPGGETVVIDPWFNGNPACPKDRQ
                     ALDRVDAILITHAHFDHMADAVDLAKRHKPKKVVASFEICSWLEGKGVENCSGMNLGG
                     SQDVLGLHVSMVRADHSSGLSDGDRILYGGLASGYMVKLPGGYT"
     gene            522..998
                     /locus_tag="VEL74_17385"
     CDS             522..998
                     /locus_tag="VEL74_17385"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HYG64355.1"
                     /translation="MSSMSLWLLFLHIIAAFWLAAGSFAGAVVRAQTKRTPELAGKVQ
                     LLRLAWRLSSVFIVPGALVAGFLGFNLVTARGWGFRPAWVHASITIYLIMLAMTLFYL
                     VPRLRKTLRAGEESLRAGAPTPEFQQLAGAKLPGILADVNALGIVILTLLMTVKPG"
     gene            complement(1081..1407)
                     /locus_tag="VEL74_17390"
     CDS             complement(1081..1407)
                     /locus_tag="VEL74_17390"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYG64356.1"
                     /translation="MSSKEMAQIRQNLEGTPDIEEAAELMNLAGNSTRLKLLYLLDNM
                     KELCVCDLAEMLGVSVSAVSQHLAKLKAYGLVAPRRDAQTIYYRLTEHAFNTKLRDNF
                     FKQFQV"
     gene            1868..2104
                     /locus_tag="VEL74_17395"
     CDS             1868..2104
                     /locus_tag="VEL74_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1901..1903,aa:Sec)
                     /transl_table=11
                     /product="SelT/SelW/SelH family (seleno)protein"
                     /protein_id="HYG64357.1"
                     /translation="MPHVRIEYCTAUNYYPRAASLAAELKKKFGLEPELVRSHGGVFE
                     VTVGNDLVFSKKKLGRFPSPGEVEKALEARSETG"
     gene            2105..3208
                     /locus_tag="VEL74_17400"
     CDS             2105..3208
                     /locus_tag="VEL74_17400"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYG64358.1"
                     /translation="MSISLITALAVGGGLLGLALRTDEQRRQAPSLPLPADSDGGDVT
                     VLLPVRDEAVNVDACLDTLLSQTAAPLVRVIDDGSTDGTAALVEARLAAEPRLSLRSA
                     GPLPDGWRGKLHALAAGARDVQTPWILSTDADTRHGPELLARARAAADEHGLDAISLA
                     GTQETRGLGENLLTPTVFALLDILLGDWGPVARGDGAPVANGQFILLQRDAWECCGGF
                     ESVRGEAIDDVAVAARLRACGYRSGFFRSPELRVRMYRGLRETARGWRRNLGGIFGPA
                     PGTTAAALAVLLVPAAPLLGFALAGAWVAAFLLWTAGAAASMILRAGAGYPPAYGLVY
                     PLDALLLAAVLILGTLDRRRGRLVSWKGREMRV"
     gene            complement(3213..>3528)
                     /locus_tag="VEL74_17405"
     CDS             complement(3213..>3528)
                     /locus_tag="VEL74_17405"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HYG64359.1"
                     /translation="FKRSLRETCARLDSRTLLPAESPRLEWSRDGDSLDVRFGSRAYR
                     LPADDTLLLPLTNTSIELLARMLWHDLAPHLAGSRVEVMAVAVEETAGQRCWYEDRLR
                     RD"
BASE COUNT          515 a         1244 c         1168 g          601 t
ORIGIN      
        1 aggtgtagcc gccgggcagc ttcaccatgt agcccgaggc cagaccgccg tagaggatgc
       61 ggtcgccgtc ggataggccg ctcgagtggt cggcgcggac catggagacg tggaggccga
      121 ggacgtcctg gctgccaccc aggttcatcc ccgagcagtt ctccaccccc ttgccctcga
      181 gccacgagca gatctcgaag ctcgccacca ccttcttcgg cttgtggcgc ttggcgaggt
      241 ccacggcgtc ggccatgtgg tcgaagtggg cgtgggtgat caggatggcg tccaccctgt
      301 ccagcgcctg ccgatccttg ggacaggcgg ggttgccgtt gaaccaggga tcgatgacga
      361 cggtctcgcc gcccggcagc tcgcagacaa cggtggcgtg gccgatccag gtgaacttgg
      421 gacgcggctg gttgttcatg gtggtctcct ccccgatcac gatggcagtg ggagggccat
      481 tctacgatag agtgcccgca tcgacgggag ggaggtttcg catgagctcg atgtcgctct
      541 ggctgctgtt ccttcacatc atcgcggcgt tctggcttgc cgccggctcg ttcgccggcg
      601 cggtggtgcg cgcccagacg aagcgcacgc ccgagttggc tggcaaggtc cagctcctgc
      661 gcctggcctg gcgcctcagc tcggtgttca tcgtgcccgg cgccctggtc gccggcttcc
      721 tgggcttcaa cctggtgacc gcccgcggct ggggcttccg gccggcctgg gtgcacgcct
      781 ccatcacgat ctacctgatc atgctcgcga tgacgctctt ctatctggtg ccgcgcctgc
      841 gcaagaccct cagggcgggc gaggagtcgt tgcgcgccgg cgcccctacc cctgagttcc
      901 agcagctcgc gggcgccaag ctccccggca ttctcgccga cgtcaatgcc ctgggcatcg
      961 tcatcctcac cctgttgatg acggtcaagc ccggctagcc tttctcgaag ctcgccccaa
     1021 aaggaaagcg gccgggggtt acccggccgc cttcggaaag gtcggggcct acgtgggctt
     1081 tcagacctgg aactgcttga agaagttgtc gcggagcttg gtgttgaacg cgtgctccgt
     1141 cagacggtag tagatggtct gagcgtcgcg gcgcggggcg accaggccgt aggccttcag
     1201 cttcgcaagg tgctgggaga cggccgaaac cgacaccccg agcatctcag ccaggtcaca
     1261 cacacacagc tccttcatgt tgtccagcag gtagagcagc ttgagccggg tggagttccc
     1321 ggccaagttc atgagttcag cagcctcttc gatgtccggc gtcccctcaa ggttctgccg
     1381 gatctgggcc atttccttcg acgacatgct cggtgcctca cctttcatag cgtgcctcca
     1441 cggtgaaaaa gttgttgaca aaagatccca aacactgccc ctcagctacg tggccacact
     1501 cctggggcca gagtgcggtc actacgttgc cctccaaggc gcgctgtcgc cagactggac
     1561 tacagcccag cgtctcgtgg gcgcgctgag ggactcccct gatgctgccc aaatggacct
     1621 gtcccgaaag gcaggtccca cgcagcgata ttgaacactc tcgattatac tcatagtcac
     1681 taacagaatt caaggggtcg tatcgatccc ccttgtcccg tttggtacgc ctcgcgcacc
     1741 tacggctttc taacacactt ctcccccgtt tgattcctca ctgaatcgcc ggcctcgcgc
     1801 aaggcctcga tcgcctccgg caccatcctc gcatccctat aatggcgtcg aaaggagggg
     1861 aaacgtcatg ccccatgtcc gcatcgagta ctgcaccgcc tgaaactact acccccgagc
     1921 cgccagtctg gcggcggagc tgaagaagaa gttcggcctc gagccggagc tcgtcaggag
     1981 ccacggcggc gtcttcgaag tcaccgtcgg caacgatctg gtcttctcca agaaaaagct
     2041 gggccgcttt ccgtcccctg gtgaggtgga gaaagctctg gaggcgagga gcgagacggg
     2101 ataggtgtcc atctccctga tcaccgctct cgccgtggga ggggggctgc tcgggctggc
     2161 gctgcggacg gacgagcagc gccgccaggc cccctcgctc cccctgcctg ccgactctga
     2221 cgggggcgac gtcacggtcc tgctgccggt gcgcgacgag gcggtcaacg tcgacgcctg
     2281 cctcgacacc ctgctctccc agaccgccgc gcccctcgtc cgggtgatcg acgacggttc
     2341 caccgacggc acggccgccc tggtggaggc gcggctcgcc gccgagccgc ggctgtcgct
     2401 gcggagcgcc ggccccctcc ccgacggctg gcgcggcaag ctgcacgccc tggcggcggg
     2461 ggcccgggac gtccagaccc cctggatcct ctcgaccgac gcggacaccc gccacggccc
     2521 cgagctcctc gcccgggccc gggcggcggc ggacgagcac ggcctcgacg ccatctccct
     2581 cgccggtacc caggagacgc gggggttggg ggagaacctc ctgacgccca cggtcttcgc
     2641 cctgctcgac atcctcctcg gcgactgggg accggtggcc cgcggcgacg gcgcgccggt
     2701 cgccaacggc cagttcatcc ttctgcagcg cgacgcctgg gagtgctgcg gcgggttcga
     2761 gtcggtgcgc ggcgaggcga tcgacgacgt cgccgtggcg gcacgtctgc gggcctgcgg
     2821 ctaccgctcc ggcttcttcc gctcgccgga gctgcgggtc cgcatgtacc gcggcctgag
     2881 ggagaccgcc cgcggctggc gccgcaacct gggagggatc ttcgggccgg cccccggcac
     2941 cacggcggcg gccctggccg tcctgctggt cccggcggcc cccctcctcg gcttcgccct
     3001 cgccggcgcc tgggtggcgg ccttcctcct ctggaccgcc ggagcggccg cctcgatgat
     3061 cctccgcgcg ggcgccggct acccgcccgc ctacggcctc gtctaccccc tggacgccct
     3121 cctcctcgcc gccgtcctta ttctagggac cctcgaccgc cgccgcgggc gcctggtgag
     3181 ctggaaaggg agggagatgc gggtgtgagg ggtcaatccc gccgcagccg gtcctcgtac
     3241 cagcagcgct gcccggccgt ttcctcgacc gccaccgcca tcacctccac ccgcgagccg
     3301 gcgaggtgcg gcgccaagtc gtgccagagc atgcgggcca gcagctcgat cgaggtgttg
     3361 gtcaagggga gcagcagcgt gtcgtcggcc ggcagacggt aggcgcggga gccgaagcgg
     3421 acgtcgaggg agtcgccgtc acgggaccac tcgaggcgcg ggctctcggc cggcagcagg
     3481 gtccggctgt cgaggcgggc gcaggtctcc ctgagggacc gcttgaag
//
LOCUS       DASPJI010000622         3965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_387033, whole genome shotgun sequence.
ACCESSION   DASPJI010000622 DASPJI010000000
VERSION     DASPJI010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387033"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VEL74_17410"
     CDS             complement(<1..240)
                     /locus_tag="VEL74_17410"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYG64360.1"
                     /translation="MIGKVVGSYKIVEKIGEGGMGAVYRAVDVMLEREAAIKAIRPDL
                     AREPQIVERFRAEARTLARLHHPAIAMIYSFFHDGG"
     gene            370..600
                     /locus_tag="VEL74_17415"
     CDS             370..600
                     /locus_tag="VEL74_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018169558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HYG64361.1"
                     /translation="MGQVTLYLDAEAEARMKEAAKASGVSLSRWLADLIREKTATEWP
                     ASIAALAGAWSDFPTAEEIRATKGEDVPRERI"
     gene            597..995
                     /locus_tag="VEL74_17420"
     CDS             597..995
                     /locus_tag="VEL74_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018951793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG64362.1"
                     /translation="MMYVLDTNSVVYFFKGMGRVSERLLNTPPRDVAIPAVVLYELEV
                     GIAKSSSPERRRSQLDELVRLVQVLPFGPAEARVTGRIRADLERIGRPIGPLDFLIAG
                     TALQYGATLVTHNVDELGRVRGLQLEDWYV"
     gene            complement(1003..1602)
                     /locus_tag="VEL74_17425"
     CDS             complement(1003..1602)
                     /locus_tag="VEL74_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HYG64363.1"
                     /translation="MCYLWSMATDPKSRVTVEQYLALERQSETRSEYLDGEVFAMTGA
                     SRKHNLLVANVLAGLHSQLRGKSCEAYANDMRVGIPGTGLYTYPDVVVACGAPQFEDG
                     ELDTLLNPTLIVEVLSGSTEDYDRGTKFAHCRTLPSLTEYVLVAQDKVHVEHFVRQTE
                     GWLLTETDDLAATLQLPSIGCTLALADVYERVFGLDAVE"
     gene            1660..3297
                     /locus_tag="VEL74_17430"
     CDS             1660..3297
                     /locus_tag="VEL74_17430"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG64364.1"
                     /translation="MPTGSIVEVGAVAADPGSVSVDGLSRLSLSNLWQAQRRFYEDEG
                     IQAWDGRIPCHATSNPLIANAYAQLIVRFLQDRAREAGRLPEPAVILELGSGAGVFGF
                     YVVKRLLELRESLGMADLPFVYVMSDLSEKNVAFWRQHPALRGLVAQGVLDFARHDVG
                     ASRPLHLLESGLVLEAGAGRPLIVLANYVFDTLPQDVFRVEGGRLLEGLTRGMPEGTS
                     LPTLDNVAVSLDQLGVSLQFREVSFPYYGNPWIDGILQRYAETLPDQGFLFPSHALLG
                     LHELVEMAGGDLLLIATDKSYGDSPPRKVSAETPELAMHDASFSLMVDFHALGQYFAA
                     RGGGAFHQPAVQATTTSVFHLGAPFERLVEARQALSTFFLEISPADLHALFSQAQIVM
                     PGSSPEVIAAFLSLARWDPALVNLHIDQLIQAAKTAGPEMRRLLAEGMRRCVDCFYYL
                     PGAESTLANAGIVFQEMQDYATALAYYRKSLEWFGQEPPEIYANTVYNMGLCHYYRGE
                     PDEALAAFREADRVALRKDMMAKGWIHHLTTEIQEGE"
     gene            complement(3306..>3965)
                     /locus_tag="VEL74_17435"
     CDS             complement(3306..>3965)
                     /locus_tag="VEL74_17435"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYG64365.1"
                     /translation="FRHHGTTLAECLYLLRRDCATVRSVVDVEGWEGVERVRAMDRPV
                     LILTGHCGNWELLAALINCLGLGMSVVARELEEPDLQKLLAGLRERFGTPTIVRGSEG
                     AARQLLLALRRGGALGMLIDQDTKVDGVWVPFFGRPAFTPVGAAKIALRQKAVVIPTF
                     IERLDDGRHRARFHPPLDLPDDPQEATALMTAAIEAQIRRHPEQWVWMHRRWRRQPPA
                     G"
BASE COUNT          636 a         1337 c         1329 g          663 t
ORIGIN      
        1 gccgccgtcg tggaagaagc tgtagatcat cgcgatggcg gggtgatgga gccgcgccag
       61 cgtccgcgcc tcggcacgga accgctcgac gatctggggt tcgcgcgcga ggtccggccg
      121 gatcgccttg atggccgcct cgcgctccag catgacgtcc accgcccgat agacggcccc
      181 catgcccccc tcgccgatct tctcgacgat cttgtagctg ccgacgacct tgccgatcat
      241 ctcgacctcg ttccccgttg aaggcgtacc ccgggattct atcgtccccg cggggatgaa
      301 acgcgcggag gggggatttc cctcggggag gatcgacagt atacttggac ttatacttgg
      361 gggtgcggta tgggacaggt gactctctat ctggatgccg aagccgaggc ccgaatgaag
      421 gaggcggcga aggcgtcagg cgtttctctg agccgctggc tcgcggactt gatccgtgaa
      481 aagacagcga ctgagtggcc cgcgtcgatc gctgccctcg caggagcatg gtccgatttc
      541 cccacggctg aagagatccg agcgacgaag ggcgaagacg tgccgcgcga gcggatctga
      601 tgtacgtcct ggacaccaac agtgtcgtct actttttcaa gggcatgggg cgtgtctctg
      661 aaaggctctt gaacacacct cccagggacg tcgccatccc tgcggtcgtc ttgtacgagt
      721 tggaagtggg tatcgccaag tcttcgtccc ccgagcggcg gcgctcccaa ctcgatgagc
      781 tggtgcgtct ggtgcaggtc ctccccttcg gtcctgccga agccagagtt accgggcgga
      841 tccgagcaga cctggagagg attggccgtc ccatcgggcc tctggatttc ctgatcgctg
      901 gtaccgcgct ccagtacgga gcgacgctcg tcacgcacaa cgtggacgag ctcggccgtg
      961 tgcgtgggct ccaactcgaa gactggtacg tctaagagct gtctactcca ccgcatccag
     1021 cccgaacacc cgctcataga cgtccgcgag ggcgagcgtg cagccgatgg aggggagttg
     1081 gagggtggcg gcgaggtcgt cggtctcggt gagaagccag ccttcggtct gacggacgaa
     1141 gtgctcgacg tggaccttgt cctgagcaac gaggacgtac tcggtcaggg aggggagcgt
     1201 ccggcagtgg gcgaacttcg ttccccggtc gtagtcctcg gtggaaccgg agagcacctc
     1261 tacgatcagc gtcggattca agagggtgtc gagctcacca tcctcgaatt ggggcgctcc
     1321 gcaggcgacc accacatccg ggtaggtgta gaggccggtg ccggggatgc cgaccctcat
     1381 atcgtttgcg taggcctcgc agctcttccc acggagttgt gaatggaggc cagccaggac
     1441 attggcgacc aggaggttgt gcttccggct ggcgccggtc atggcgaaaa cttcgccgtc
     1501 gaggtactcg ctccgggtct cggactgcct ctccagggca agatactgct cgacggtgac
     1561 gcgagacttc gggtccgtgg ccatggacca aaggtaacac accgcttgcc cgcctcccgg
     1621 cgggctcgta gactacgaga gcttgcgaga gagtggacga tgcccacggg ctcgatcgtg
     1681 gaagtaggcg ccgtggcggc ggatcccggg agcgtgtctg tcgacggtct ctcccggctt
     1741 tcgctcagca acctctggca ggcgcagcgg cgcttctatg aggacgaggg gatccaggcc
     1801 tgggacggca ggatcccctg ccacgccacc agcaatcccc tgatcgccaa cgcgtacgcg
     1861 caactgatcg tccgcttcct ccaggaccgc gcgcgcgagg ccggccgtct gccggagccc
     1921 gcggtcatcc tggagctcgg cagcggcgcg ggggtgttcg ggttctacgt ggtgaagcgg
     1981 ctgctcgagc tccgcgagtc gctcgggatg gctgacctgc cgttcgtcta cgtgatgagc
     2041 gacctgtcgg agaagaacgt cgccttctgg cggcagcacc cggctctccg cggcctcgtc
     2101 gcccagggcg tcctcgactt cgcccgccat gacgtcggcg ccagccgccc gctccacctc
     2161 ctggagtccg gcctggtcct cgaggccggc gcaggccggc ccttgatcgt cctcgccaac
     2221 tacgtcttcg acacgctccc gcaggacgtc ttcagagtgg agggggggcg gctgctggag
     2281 gggttgaccc ggggcatgcc cgagggcacc tccctgccca cgctggacaa cgtcgcggtg
     2341 agcctcgacc agctcggcgt ctccctgcaa ttccgcgagg tctcgttccc ctactatggc
     2401 aatccctgga tcgacggcat tctccagaga tatgccgaga cgctcccgga ccagggcttc
     2461 ctcttcccca gccacgcgct cctcggcctg cacgagctcg tggagatggc cggaggggac
     2521 ctgctgctga tcgccacgga caaatcctac ggcgacagcc cgccgcggaa ggtctcggcc
     2581 gagacgcccg agctggcgat gcacgacgcc tcgttctccc tgatggtcga cttccacgcc
     2641 ctcggccagt acttcgcggc ccgcggcggc ggcgccttcc accagccggc ggtccaggcg
     2701 accacaacct cggtcttcca tctcggcgcc cccttcgagc ggctcgtgga ggcgcgccag
     2761 gccctgtcga ccttcttcct cgagatcagc ccggcggacc tgcacgcact cttcagccag
     2821 gcgcagatcg tgatgccggg aagctcgcca gaggtgatcg cggccttcct gagcctcgcc
     2881 cgctgggacc cggcgctggt caacctgcac atcgaccagc tcatccaggc ggccaagacc
     2941 gcgggccccg agatgcgccg gctcctcgcc gaaggcatgc ggcggtgcgt ggactgcttc
     3001 tattacctgc ctggagccga gtcgaccctg gcgaacgccg gcatcgtctt ccaggagatg
     3061 caggactatg ccacggccct ggcctactac aggaagtcgc tggagtggtt cggccaagag
     3121 ccgccggaga tctacgccaa tacggtgtac aacatggggc tctgccacta ctaccggggc
     3181 gagccggacg aggcgctggc agcgttccgt gaggcagacc gggtcgcgct ccgcaaggac
     3241 atgatggcca aggggtggat ccaccacctc acgacggaga tccaggaggg ggagtaggcg
     3301 ggccctcagc ctgccggcgg ctggcgccgc cagcggcggt gcatccagac ccactgctcg
     3361 gggtggcggc ggatctgggc ttcgatggcc gcggtcatca gggcggtggc ctcctgggga
     3421 tcgtccggca gatcgagcgg cgggtggaac cgggcgcggt ggcggccgtc gtccaggcgc
     3481 tcgataaagg tggggatcac cacggccttc tggcgcaggg cgatcttcgc cgctcccacc
     3541 ggtgtgaagg cggggcggcc gaagaagggg acccagacgc cgtcgacctt ggtgtcctga
     3601 tcgatcagca tccccagggc gccgccgcgg cggagcgcca gcaggagctg ccgcgccgcg
     3661 ccttcgctgc cgcgcacgat ggtcggggtg ccgaaacgct cccgcaggcc ggcgaggagc
     3721 ttctgcaggt ccggctcctc cagctcccgc gccaccaccg acattcccag gcccaggcag
     3781 ttgatcagcg ccgccagcaa ctcccagttg ccgcaatgac cggtgaggat caggaccggc
     3841 cggtccatcg cccgcacccg ctccaccccc tcccacccct cgacatccac gaccgagcgc
     3901 accgtggcgc agtcgcggcg cagcaggtac aggcactccg ccagcgtcgt gccgtgatgc
     3961 cggaa
//
LOCUS       DASPJI010000623         5002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_96806, whole genome shotgun sequence.
ACCESSION   DASPJI010000623 DASPJI010000000
VERSION     DASPJI010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96806"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5002)
                     /locus_tag="VEL74_17440"
     CDS             complement(<1..>5002)
                     /locus_tag="VEL74_17440"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64366.1"
                     /translation="RRHEALRTTFSLGEDGEPVQVIAPAGPADLPVADLSGLPPATRE
                     AEGVRLAREEAWRPFDLAAGPLLRAALVVLGSEQHLVLLAMHHIVSDAGSMGILTREL
                     AALYGAFSSRLPPQDLPLPGLPVQYADYAVWQRQVLRGETLAAQLAYWRSHLAGAPPV
                     LALPTDRPRPAVQTFRGGGHSFALPPALAADLRTLALQRQATTFMTALAAFQALLSRW
                     AGQPDVSLGTPIAGRGRSEVEGLIGLFLNTLVLRVRVDEEEPFSGLLRRVREVTLGAY
                     THQELPFEKLVEELQPQRDLAHSPLFQVMFTLEAEEAAPPADGLQLEAVPLGSGLAKF
                     DLTLSLQESPHGIAGFAEYNRDLFDGTTIERMLGWFQVLLAAAAASPDLPLAGLPWMT
                     GAERQQVTAEWSGMDAGVQDEALAPWRIARQAAAAPEAPAVGIGDAWLSYGELEAEAR
                     RLAGRLRRLGVGRGDRVAVLAERSPALVAALLGVWRTGAAYIPLDPSHPRERLAAILD
                     DALGGVAHPVIVTDQDEVPGASATVLRVTDEDGPAPERDAVELSADDIAYVIYTSGST
                     GRPKGVEVRHGGLAAFLDAMARRPGLAAGDVLLAVTTVSFDIAALELFLPLAVGARVA
                     LASRETAGDGALLAEEVRRSGATMLQATPSTWRMLLSSGWQGAPGLTALCGGEALPAD
                     LATALLTRVGALWNVYGPTETTVWSTVHLVEAEDGTVSTVSIGRAIAGTTVHLLDRRF
                     EPVPPGVVGDLFLGGAGLARGYLGRPDLTAAAFVPDPWGDNPWGDGARLYRTGDLARQ
                     RADGSLDFLGRADLQVKVRGVRIELEEIETVLRSHPGVREAAVVARPEPGGGASLLGC
                     VVPGTETAPASATLRDFLRAKLPDSMVPSGWVFLQSLPLTPSGKVDRRALADLPPISA
                     ATPEGTGAEAPRGPVEEILAAAFAQVLRLGRVGRDDDFFALGGHSLLATRLIARIRHD
                     LGTELPVRAVFEASTVAALSRLLERERSAAGAAPPLVPVPRQGDLPLSFAQERLWFVD
                     QLQPGNAVYNLPMSVPLEEGLDPRALEGALGEVVRRHEALRTSFPAAGGHPVQRIAPA
                     VGWLLPRVDLGGLPEERRLSEASRLAADEAARPFDLAAGPVLRSLLLVGAGGVGARLL
                     LTMHHIAADAWSMELLVNEMETLYRAFASREPSPLPEPELQYADFAVWQRRWLAGAEL
                     ERQLAYWRHRLAGAPPSLDLPTDRPRPAVQSYRGTRRVLRLAATLDQNLRTLAREHGS
                     TLFMTVLAGVAALLSRWTGQDDLVIGTPIAGRGQVELERLIGFFANTLPLRVDAGGSP
                     SFAELLLRVRDTALEAYAHQDLPFEKLVEELRVERDLSRPPVFQVVLSLPNTGAARRP
                     AGDGVDGGPGLPAGGEEAAIARFDLTLEVREGPAGLDVEAEYATALFDGATIERLLGH
                     LERLLALVARDPRQPAPRPTLPVTELPLLDPAELQQAIREWNDTAAPYPAEADLFALF
                     AERARSAPDAVAVESGSACLSYSSLLRRATALARRLRQAGAGPESRIGLGLERSPEMV
                     VALLAIVQVGGAYVPMDPALPQERLTAMAWDAGIEVLVAREPLLGFLAPFPLVEPEPL
                     EEAEPGPDLPLLPEGGDRLAYVVFTSGSTGTPKGVAVPHRAVVRLVWETDYAAFGL"
BASE COUNT          717 a         1847 c         1879 g          559 t
ORIGIN      
        1 tccaggccga acgccgcgta gtcggtctcc cacaccagcc gtaccaccgc ccggtgcggc
       61 acggcgacgc ctttgggcgt gcccgtcgag ccggaggtga acaccacgta ggcgaggcgg
      121 tccccaccct ccggcagcag cgggagatcg ggcccgggct ccgcctcctc cagcggctcg
      181 ggctcgacga gcgggaatgg cgcaaggaat cccagcaggg gctcgcgggc gaccagcacc
      241 tcgatgccgg cgtcccacgc catggcggtc agccgctcct gcggcaaggc ggggtccatg
      301 ggcacgtagg ccccgcccac ctggacgatg gccagcaacg ccaccaccat ctccggcgag
      361 cgctccaggc cgaggccgat gcggctctcc ggcccggctc cggcctgccg cagccgccgg
      421 gccagggccg tggcgcgccg gagcagagag ctgtagctca gacaagcgct gccggactcc
      481 acggccaccg cgtccggcgc gctccgcgcc cgctcggcga acagggcgaa gaggtccgcc
      541 tccgccgggt agggcgcggc ggtgtcgttc cattcgcgga tcgcctgctg gagctcggcg
      601 gggtcgagca gcggcagctc ggttaccggc agcgtcggcc gcggcgccgg ctgcctgggg
      661 tcccgcgcga ccagggcgag cagccgttcc agatgcccca gcagccgctc gatggtggcg
      721 ccgtcgaaca gcgcggtggc gtattcggcc tcgacgtcga ggccggcggg gccctcccgc
      781 acctccaggg tgaggtcgaa tctggcgatg gcggcctcct ctccaccagc cggcaggccg
      841 ggaccgccgt ccacgccgtc gccagccggc cggcgcgccg cgcccgtgtt gggcagggac
      901 agcaccacct ggaagacggg cgggcggctc aggtcccgct ccacccgcag ctcctccacc
      961 agcttctcga agggcaggtc ctgatgggcg taggcttcca gtgccgtgtc gcgcacccgc
     1021 agcagcagct cggcgaagct cgggctccca ccggcgtcga cgcgcaacgg cagggtgttg
     1081 gcgaagaagc cgatgaggcg ctccagctcc acctggccac ggcctgcgat gggcgtgccg
     1141 atgaccaggt cgtcctgacc ggtccagcgg ctcagcagcg ccgccacgcc ggcgagcacg
     1201 gtcatgaaga gcgtcgagcc gtgctcgcgg gcgagcgtcc ggaggttctg gtccagcgtg
     1261 gctgcgagcc gcagcacccg ccgggtgccg cggtagctct gcacggccgg ccgcggccgg
     1321 tccgtgggca ggtccaggga gggcggcgcg cccgccaggc gatggcgcca gtaggcgagc
     1381 tgccgctcga gctccgcgcc cgcgagccac cgccgctgcc agaccgcgaa gtcggcatac
     1441 tggagttccg gctcggggag cggcgacggc tcgcgagaag cgaaggccct atagagggtc
     1501 tccatctcgt tcaccagcag ctccatggac caggcgtccg cggcgatgtg gtgcatggtg
     1561 aggagcagcc gcgcccctac cccgccggcc ccgaccagga ggaggctccg cagcaccggc
     1621 ccggcagcga ggtcaaaggg gcgggcggcc tcgtccgcgg ccagacgcga cgcctcggag
     1681 aggcgccgct cttcgggcag gccgccgagg tcgacgcggg gcaggagcca accgacggcc
     1741 ggagcgatcc gctggacagg atggccgccc gccgccggga aactggtgcg gagagcctcg
     1801 tggcgccgca ccacctctcc cagcgcgccc tcaagggcgc ggggatcgag gccctcctcc
     1861 agcggcaccg acatcggcag gttgtagacg gcgttccccg gctggagctg atcgacgaac
     1921 cagaggcgct cctgcgcgaa cgacagagga agatcgccct gccgcggcac cggcacgaga
     1981 ggaggcgcgg cgccggcggc gctccgctcc ctctccagga ggcgggagag ggcggccacg
     2041 gtcgaggcct cgaacaccgc ccgaaccggc agctcggtcc cgaggtcgtg gcggatccgc
     2101 gcgatgagcc gggtcgcgag aagggagtgc ccgcccagcg cgaagaaatc gtcgtccctg
     2161 cccacgcggc cgaggcgcag gacctgggcg aaggcggcgg ccaggatctc ctccaccggg
     2221 ccccgcggcg cctccgcccc ggtcccctcc ggcgtggccg ccgatatcgg cggcaagtcc
     2281 gccagggcgc ggcggtccac cttgccgctc ggggtgaggg gcaaggactg gaggaacacc
     2341 cagccggagg gcaccatcga gtccggcagc ttggcccgca gaaagtcccg cagtgttgcg
     2401 gatgccgggg cggtctccgt tcccggcacc acgcagccga gcaggctcgc gccgccgccg
     2461 ggctcggggc gggccaccac cgccgcctcg cggacccccg ggtgggaccg caggaccgtc
     2521 tcgatctcct cgagctcgat gcgcacgccg cgcaccttga cctggaggtc ggcgcgaccg
     2581 aggaagtcga ggctgccgtc cgcccgctgg cgcgccaggt cgcccgtccg gtagaggcgc
     2641 gcgccatccc cccagggatt atccccccag ggatcgggga cgaaggcggc ggcggtcagg
     2701 tccggacgcc cgaggtagcc gcgggcgagg cccgctccgc cgaggaagag gtcccccacc
     2761 accccgggag gcaccggctc gaagcgccgg tcgagcaggt ggacggtggt gccggcgata
     2821 gcccgcccga tggatacggt ggagaccgtt ccgtcctcgg cctccaccag gtggaccgtc
     2881 gaccagaccg tggtctcggt ggggccgtag acgttccaga gcgcgcccac ccgcgtcagg
     2941 agggccgtgg cgaggtcggc cggcagcgcc tcgccgccgc acaacgcggt caggcccgga
     3001 gccccctgcc agcccgacga cagcagcatc cgccaggtgg acggagtcgc ctggagcatt
     3061 gtggcgccgg aacggcggac ctcttccgcc aggagggcgc cgtcgccggc cgtctcgcgg
     3121 ctcgccagcg ccacccgcgc ccccaccgcg agcggcagga acagctccag cgccgcgatg
     3181 tcgaaggaga cggtggtcac cgccaggagg acgtcgccgg cggcgaggcc gggccgccgg
     3241 gccatggcat cgaggaaggc ggcgaggccg ccgtgccgga cctcgacccc cttcggccgt
     3301 cccgtggagc cggaggtgta gatgacgtag gcaatgtcgt ccgcggagag ctccaccgcg
     3361 tcgcgttccg gcgccggccc atcctcgtcc gtcacccgca gcaccgtcgc cgaagcgccc
     3421 ggcacctcgt cctggtcggt gacgatcacc gggtgggcga cgccgccgag cgcgtcgtcc
     3481 agaatggcgg cgagccgctc ccgcgggtgg gacggatcca gggggatgta ggcggcgccg
     3541 gtccgccaga ctcccagaag agcggcgacc agcgccggcg agcgctccgc cagcaccgct
     3601 acccggtcgc cgcggccgac gccgagccgg cgcaggcgac cggccagacg cctggcctcc
     3661 gcttcgagct ctccatagga gagccaggcg tcgccgatcc ccacggccgg cgcctcggga
     3721 gccgccgcgg cctgccgggc gatccgccag ggggccagag cctcgtcctg gacgccggca
     3781 tccatgccgc tccactccgc cgtcacctgc tgccgctcgg cgccggtcat ccaggggagg
     3841 ccggccagcg gcaggtccgg ggaggccgcg gcagcggcca ggagaacctg gaaccaaccg
     3901 agcatccgct cgatggtcgt gccgtcgaac aggtcgcggt tgtactcggc aaaaccggcg
     3961 atgccgtggg gcgattcctg aaggctgagc gtcaggtcga acttcgccag gccgctcccc
     4021 agggggacgg cttcgagctg cagcccgtcc gccggcggtg ccgcctcctc cgcttccagg
     4081 gtgaacatca cctggaagag cggcgagtgg gcgaggtcgc gttgtggctg cagctcctcc
     4141 accagcttct cgaagggcag ctcctggtgg gtataggccc cgagggtcac ctcccgcacc
     4201 cggcgcagga gaccggagaa cggctcctcc tcgtcgaccc tgacgcgcag caccagcgtg
     4261 ttgaggaaga gaccgatgag cccctccacc tcggagcggc cgcggccggc gataggggtg
     4321 ccgaggctca cgtcaggctg cccggcccag cgggagagga gggcctggaa ggcggcgagg
     4381 gcggtcatga aggtcgtcgc ctgccgctgg agcgccaggg tccggaggtc cgccgcgagc
     4441 gcggggggca gcgcgaagga atggccgccg ccgcggaacg tctggacggc ggggcgcggc
     4501 cggtcggtgg ggagcgccag cacgggcggc gcgccggcca gatggctgcg ccagtaagcg
     4561 agctgcgccg ccagcgtctc cccccgcagg acctgccgct gccacaccgc atagtccgcg
     4621 tactggaccg gcagcccggg cagtggcagg tcctgcggag ggagacggct cgagaaggcg
     4681 ccgtacagcg ccgccagctc gcgggtgagg attcccatgg acccggcgtc gctcacgatg
     4741 tggtgcatgg cgaggagcac caggtgctgc tccgagccga gcaccaccag tgcggcccgg
     4801 aggagcggac cggcggcgag gtcgaacggg cgccaagcct cctcgcgggc gagccggacc
     4861 ccctcggcct cgcgggtggc cggcggcagg ccggagaggt ccgccacggg caggtccgca
     4921 ggcccggcgg gcgcgatgac ctgcaccggc tcgccgtcct cccctaggga gaaggtggtt
     4981 cgcagcgctt cgtgccgccg gc
//
LOCUS       DASPJI010000624        22289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_40141, whole genome shotgun sequence.
ACCESSION   DASPJI010000624 DASPJI010000000
VERSION     DASPJI010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 22289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22289)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22289
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40141"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VEL74_17445"
     CDS             <1..573
                     /locus_tag="VEL74_17445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG64367.1"
                     /translation="PDMVLLRLIGERRPEAVSRLYDRHAPMLLALARRILGSTADAEE
                     VLQEVFLHVWDHCGRYDPARSSVSTWLVLLTRSRAIDRLRHRNVVERTHEQAHASGTS
                     EGTDPRDDASPGGPDAVLIRERRERVRRELEGLPPEQRQVLEMAFYEGLSQSEISARA
                     GLPLGTVKTRTLLAMKKLRNALRMEIRELL"
     gene            570..1568
                     /locus_tag="VEL74_17450"
     CDS             570..1568
                     /locus_tag="VEL74_17450"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HYG64368.1"
                     /translation="MSQETPEDRTILSALDAPDGGIGVSAHTGLPKTARDETAETLAR
                     LYTEVLGLLPLELEPRAPRPELKERLMASLAAVATPAVADEVQAPAPALPAAEPPVAS
                     REVATEPAAPPAAPPEPRPSQEVRIPHPATLPAPPPPPPRRWPLALAAALALIFAGLS
                     GVLGYLVSEQRVRMNQLEADLDKARDAETRARRAEAQADRAHADMLKMRESLSLVTTP
                     SVLVMPLRPQGDSAPQPDAKGVLFVAADHQHWHLALHQLRPAEPGRQYQLWFATDDGR
                     TVSGGTFTVRGPTVTMSSPTMPTGTVAASITVEPAGGSISPTGPEVLKTNEAFQLS"
     gene            complement(1569..2306)
                     /locus_tag="VEL74_17455"
     CDS             complement(1569..2306)
                     /locus_tag="VEL74_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG64369.1"
                     /translation="MSPMEEKKVALVTGGSRGIGRAIVEALLGEGWRVHLCSRTPAST
                     PGSVAEAVRELAGRFGDAVAGEAVDARRQEEVDGFVERVLAREGRIDGLVNNAGVGLF
                     NPVDEITGEQWREVIETNLSGPFYFTRAVAPAMKRQGSGWIFNIASLAGRNAMAGGAA
                     YNASKFGLVGMSEASMLDLRQHGIRVASILPGSVDTGFGHPDRTGDRSWRLQPEDVAA
                     MVLHLLTYPPHALPSLVEMRPSRPPGK"
     gene            2419..3144
                     /locus_tag="VEL74_17460"
     CDS             2419..3144
                     /locus_tag="VEL74_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HYG64370.1"
                     /translation="MYLDPIYFAFILPALALSLWASWRTKSAFNKYSKVRTYRGMTGA
                     QAAQEMLRAANVQGVEIVPTHGMLTDHYNPVSKKLALSEAVYASNSVAAVGVACHEAG
                     HAIQHAQNYMPMWIRSMLVPVANIGSTVGYIVMSLGLMLMYMSRSAESPGMGQGIVLF
                     GAVLFSAVLLFQIVTLPVEFDASARAKRLAVETGIVLPQEREGMDKVLNAAALTYVAA
                     AVSTLMTLLYYLLRAGLLGGRRE"
     gene            3263..4204
                     /locus_tag="VEL74_17465"
     CDS             3263..4204
                     /locus_tag="VEL74_17465"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HYG64371.1"
                     /translation="MRYLLILVTAALLTVPAAAQTAPAPKTPPADDHSSHAHAAPAAP
                     ATPATPANTAAAPRDENLPPGEQQAKAALEKSPRHGELVDVKLPAGGTPIRTYVVYPE
                     RKEKAGVVIVIHEIYGLTDWIRGVADQLAREGFIAVAPDLISGLGPNGGGTDSVASRD
                     DVTKLVRGISPEDATARLNAVRDYAIKLPAANGKSATVGFCWGGARSFAYAAAQPGLN
                     AAVVYYGTSPENAADYARIKAPVLGLYGEDDARVNATIPPAEAEMKKAGKSYEPHIYK
                     GAGHGFLRAQDGRDGANLKATQQAWPRTVAFLRQHLR"
     gene            4257..5303
                     /gene="selD"
                     /locus_tag="VEL74_17470"
     CDS             4257..5303
                     /gene="selD"
                     /locus_tag="VEL74_17470"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:4311..4313,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HYG64372.1"
                     /translation="MAWFRRKFRLTACATTGGUAAKVGPGDLSTLIGPLVRLAGRDER
                     VLVGPETADDAGVYLLGDRALIATTDFITPVCDDPRRFGRVAAANSLSDVYAMGGRPL
                     FALNLCCFPEEVPGDALAGILEGAAAALAEAGAALLGGHSVRDPELKFGLAVTGEGDP
                     NRLLTNAGARAGDRLLLTKPLGTGVLVNAFKFDKLDEAGLEPALLEMERLNAVASRLA
                     LEHGVHAATDITGFGLAGHAFGMARASGVGVRIAFGRLPVYDAFYPLAKSGVTTGCTR
                     ANEDNVHQAFTDHAGLDKVQRDVLFDPQTSGGLLLSTPPEQAAPLLAALRETGHRAAE
                     IGEVVAGPPRLEVV"
     gene            complement(5300..5962)
                     /locus_tag="VEL74_17475"
     CDS             complement(5300..5962)
                     /locus_tag="VEL74_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HYG64373.1"
                     /translation="MTSTRTPRQLDPVEIRVLGVLLEKEQTTPEACPLTVNAVLAGAN
                     QKTNRDPVMELTEEQVVDALDRLRQVVLAWRTEGARTERWQQSVVRRWGLDRAGKALM
                     TLLLLRGAQTPGELRSRSDRLHPFASVEEVEAALQGMAGMDEPLVRELPRRPGQKESR
                     WIHLVGEVAEPAPEPAYTAGAAGLPAERPDRPSLAARVERLESTVAQLADELKALKDL
                     LS"
     gene            6436..6642
                     /locus_tag="VEL74_17480"
     CDS             6436..6642
                     /locus_tag="VEL74_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HYG64374.1"
                     /translation="MPEQGTVKWFNDAKGYGFIVRERGADVFVHHASIIAEGFRTLSE
                     GDRVAFDVVEGPKGLQAKNGVKVS"
     gene            complement(6733..7554)
                     /locus_tag="VEL74_17485"
     CDS             complement(6733..7554)
                     /locus_tag="VEL74_17485"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYG64375.1"
                     /translation="MFSKAHTSSFARPAAMVAMTFLALTLCTADAQAQKRGLNLVSLD
                     QEWQMRSELHQQVQREARLVNDRQALAYLNTVGRRLAAQTPYANRPWDFFIVNDPSVN
                     AFNLPGGLVYVNTGLIAEADTLDQLAGVLAHEIGHGAARHGTQLMTRAYGYNLVAGLL
                     LGRNPGQFQQLLAQLGGVTALNAYGRDAEREADRLGVQYAYRAGYNPDGVADFFRQLL
                     DMRQRRPTKVEQFFSNHPMTETRIRNVEAEAAGLSRRGNLTRDTKAYQQFRARFR"
     gene            7847..9238
                     /locus_tag="VEL74_17490"
     CDS             7847..9238
                     /locus_tag="VEL74_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HYG64376.1"
                     /translation="MRRAVIAVVFLFLAFQAWGPRLAEASEAGGGGHGGPVVPVLLGL
                     VLILSAAKLGGELFERMGQPAVLGELICGMLLGNLALLGLPGFDFLHTTAGLEILAQL
                     GVILLLFQVGLESNVKEMLSVGWSSLLVAVLGVIAPFFLGWGVSAWLLPEEPRLAHIF
                     IGATLCATSVGITARVLADLGKLAARESKIVLGAAVLDDVLGLVVLSVVSGTITAANT
                     GSSLDPMDAGAIILKSLAFLVGAVALGGWLSPRVFRLASRLKIRGLLLAISLAFCFLL
                     AYLADRIGLAAIVGAFAAGLILDEVHYKDLRDRGEHNLEELLHPIAGFLVPVFFVLMG
                     VKVDLRSFGQVEVLGFAALLSLAAVLGKMICAAGVLERGLDRVSVAVGMVPRGEVGLI
                     FASIGAQLVLGGQRVITPPVFGAVVVMVIVTTLITPPVLKVTLARGDRKKAAALKPPG
                     TDEPMDRPNPSLG"
     gene            complement(9481..9870)
                     /locus_tag="VEL74_17495"
     CDS             complement(9481..9870)
                     /locus_tag="VEL74_17495"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HYG64377.1"
                     /translation="MVLFLLLLGVASLKSSRDLEASRGREALLENRIHETEARIERLH
                     ARIDRLRKDPGALERHAREDLGMVRPGDLVIIVPDGPPGADTPRPAPRSEPLVPAPTP
                     VPPPAATPPVATPVTASSPAPAASAPL"
     gene            complement(9930..11231)
                     /gene="eno"
                     /locus_tag="VEL74_17500"
     CDS             complement(9930..11231)
                     /gene="eno"
                     /locus_tag="VEL74_17500"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HYG64378.1"
                     /translation="MFCIEEIVAREILDSRGNPTLEVDCILDGGVGGRAAVPSGASTG
                     EREALELRDGGKRYRGKGVSKAVGHVMDEIGPTMRGLDARDQARIDRALIELDGTPDK
                     SRLGANALLGVSLAVAKAAAEASGLPPYAYLGGPAATVLPVPLLNVLNGGAHADNSVD
                     IQEFMIVPMGFETFPEALQAGVEIYHTLKDVLKGRKLVTAVGDEGGFAPDLKSNREAL
                     DLLMQAIEKAGYTPGRQVGLALDVAASELVEGKGCKNVKYALAGEGKKGLASSDLIAL
                     YREWLDAYPLVSIEDGLAENDHAGWKELTRELGSRVQLVGDDLFVTNPEILAKGIAEG
                     MANALLVKVNQIGTLTETLEAVDMAKRNAYANILSHRSGETEDTTIADLAVATRAGQI
                     KTGAPCRSDRVAKYNRLLRIAEELDVVATYPGAAAFPRWSP"
     gene            11413..12051
                     /locus_tag="VEL74_17505"
     CDS             11413..12051
                     /locus_tag="VEL74_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64379.1"
                     /translation="MFSKARRSDSSQPIRLHIGSGKVRLPGWLNVDIQALPGVDVVAD
                     VTKGLHYTDVEAIFAEHFLEHLAIDDALKFLQESHHALAAGGWIRLSTPNLDWVWVTH
                     YRLEADEEMKRTYALGLNRAFHGWRHRFLWNREMLGEALLACGFEEPRWCRYGESGLD
                     LFQGIEHHEVYQDAPELPHVIIAEARKGEPRPERLRAFEERIREGFLNHMAD"
     gene            12064..13560
                     /locus_tag="VEL74_17510"
     CDS             12064..13560
                     /locus_tag="VEL74_17510"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HYG64380.1"
                     /translation="MSTSTPPSARVARILAVVASTALAVAAVCAWGWYRAARDRVPLE
                     SLGPEDRQALVQEMLAASPGAFAPALFEPAIGYTLRPGEEIEAWGDTFTSNELGYRTG
                     PPGKPKRTFRVVFIGDSWTFGLGVSEAESFPSQLEALARQAGAAGPGGRRQVEVRNLG
                     LPGYNTMNELAALDFFFDRLRPDAVVICPTANDADSASNILPNGSLTRAGVERDGYGA
                     DHSMVFRTRLADSYLFRTRWRAAFAGIRGLEERLRRRGVPLLVYFAASWDEPFAHALV
                     ADAGLDAPYVVTPPGFAGPRWRNPPPWRHGTPEANRIYARMVYNGLAELLGWPAQPKE
                     EGAEVPVYRRGTAGDGSARAEELLREETRRIPESYVPGPDAEIQCVGAMDWTTGLTGK
                     ATTVLVRRRAGARRAAVTLRRLPDAPSLYPLAVRVSIPSAAGGTEAESTLPADGPGIT
                     DLQRVVLDIPGDLPEGAALDITIRAARAAAAPGVLAPRSMYIAGIEQE"
     gene            13566..15005
                     /locus_tag="VEL74_17515"
     CDS             13566..15005
                     /locus_tag="VEL74_17515"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYG64381.1"
                     /translation="MWKNPPPDLPHSLPPTTQGGRHHHPCWLGLMGLALVFFGACAPS
                     PPPPQPRNVVLILVDTLRADHLGAYGYGRPTSPNLDAFARGAVLFQDARSQASCTFPS
                     ANSLLTSRYPAAFMGQPEQAMGIPGGIPSVAEVLQGKGYRTVAVSASPIVRKSPTRFN
                     PQGGFARGFETFSEDCLWKPAECVNRVTMEQLEQLEKEEDGRPFFLYLHYMDPHGPYA
                     PPPGHVRRFATGSPDKAFIREGNPNPIGDMLYKGAPDPGATPADLQHLVGLYDDEIAY
                     FDAQLALLLQRLRETGRMEDTLVVLTSDHGEEFLEHGHIKHCRTLFDTSIRIPLILHV
                     PGAAPRTVTRPAENVDVVPTLLDLLGIGTEGLRLEGRSLRPLLGDEESDESPDHQFSA
                     QGPLRSVADGGFKLVEDLAGGSVALYDLRADPGETKDVLRDRRRDFHRLRQALTAWLA
                     ATEGQHAAGESVHQAEEAERKLRALGYIE"
     gene            15139..15215
                     /locus_tag="VEL74_17520"
     tRNA            15139..15215
                     /locus_tag="VEL74_17520"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15173..15175,aa:Met,seq:cat)
     gene            15391..15687
                     /locus_tag="VEL74_17525"
     CDS             15391..15687
                     /locus_tag="VEL74_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64382.1"
                     /translation="MAGCPALDLFSQGQSEEEAKRSLEEAIGLWVEDCLERGTLDQAL
                     RELGFDRVHPGTTVSPGDKPIPAGAVSEDAVPAPATFPVHISIPTYRAAALLAS"
     gene            15784..15939
                     /locus_tag="VEL74_17530"
     CDS             15784..15939
                     /locus_tag="VEL74_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64383.1"
                     /translation="MSRPGSARPVVIKMDADLGEDIIQSNKRTMGLTTAEFEALLEQV
                     RGKRKPR"
     gene            complement(15956..17176)
                     /locus_tag="VEL74_17535"
     CDS             complement(15956..17176)
                     /locus_tag="VEL74_17535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYG64384.1"
                     /translation="MPAYVRQIWILMATAFVDMIGFTMVQPLLPFYATELGARPWVVG
                     ALTTAYAFAQFSTAPLWGRMSDRYGRRPMILAGLVFSAVAFALFGLATTLWMLLAFRL
                     VQGAAGGTIGVVQAYVADSMGPEERAKGLGWLTAATSAGVMIGPAVGSLASTFGQEAP
                     GFIAAGLCLMNFLFASRWLPEPPRHQEAGVPRRLRRAVVDVLLHPSGPVPSLIWIYAI
                     GMMAFFSMTTILPLYLGAAFGVTERTIGWFFVYVGAVGVIMRALLLGPAVRRFGEVGV
                     VRLGALSLIVGLACIPLPAQVDLPYAGRVTLLTVVALFVPVGTALLFPSTTALVSRRS
                     ARGETGQTLGVQQAFGGVSKMLGPLWAGVVFQIGIEYPFWVAAALMLGVGTLTLKLRP
                     QEPEPYPLPAGSKP"
     gene            complement(17207..18859)
                     /locus_tag="VEL74_17540"
     CDS             complement(17207..18859)
                     /locus_tag="VEL74_17540"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HYG64385.1"
                     /translation="MSSRERLLVPALLAVLLAWLVGYPLLITLMEALGGPRAWTTGHF
                     AAFAGRPDEWLALWRSLWISAASVVLSALVGVPLAFLFERTEFPGRRFLGAVAALPVA
                     LPPLVGVIAFLFLYGESGFISRAVQAGLGLDGPPWRLVGPGAILLVHTYSMYVYFYLF
                     TRAALARQDAALLEAAASLGAGRVRTFFRVTLPLLRPALAGASLLTFMTSLASFSAPY
                     LFGGTFRVMTTQIMASRLNGELALAQVETVMLAGLALLALWAMRRADRAASTGTGVRG
                     IAPTRRRLRSPAARWTAGALGYLLAALLLLPHAVLALVSLVPAFTWTAEPFPPVLDFG
                     NYRALFTDAERLRPVLNSLWMALASTAAALVLGFAAARAALARKGSLGGALEGLLGLP
                     WAIPGTVFAVALATTFSVQQPAVGRFVLIGTPLILPLAYLVRSLPLTGRAALAGLRGL
                     DPALDEAASSLGAGRLRRLTRVTLPLLRPALAAGAGLAFITALGDFVVSIVLYTFDTR
                     PISIEILSSLRLQDLGMAAVYGVLLMVASTVAFLLWGQGDVR"
     gene            complement(18863..19948)
                     /locus_tag="VEL74_17545"
     CDS             complement(18863..19948)
                     /locus_tag="VEL74_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG64386.1"
                     /translation="MPFLELDRLDKSFSSNRVLRELSVAVEQGQILALLGPSGSGKTT
                     ALRIIAGFETPDAGHLRVGGEDVTTFPPARRNFGMVFQHYALFPHLTVGENVAFGLES
                     RGKTDRADIRRRVSEALALVDLPGFEGRRVGEISGGQQQRVALARALAPEPRVLLLDE
                     PLSNLDPTLRERTRRELKAAIQRVGITTVLVTHEQEEAFDLGDRVAVLQGGILHQVGS
                     PEDLYERPATRFVAGFVGRAAALPGTFRGGQVVLDGGVSWPGVPAEELAEGSPAELVV
                     RPESLRFASPGTAGALPGKVAERRYAGPVTYFVVALESGREVEVMAAPDAAAPGDAVA
                     VAPGGAGPKPRIFRASSRGEGPSRDVG"
     gene            complement(19952..21040)
                     /locus_tag="VEL74_17550"
     CDS             complement(19952..21040)
                     /locus_tag="VEL74_17550"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYG64387.1"
                     /translation="MKPTRFLSGVALALPFLPLLISGCGDGRTPLVVYSPHGRDLLSL
                     MEKSFEQSHPDVDVRWLDMGSQDVYDRVRSEAANPQADVWYGGPRAIFAQGEREGLLA
                     PYRPRWADAVPPESRHPQDLYFGSYRTAPVLVFNKQAVPEAEAPRDWDDLLDPKWTGK
                     ILMRDPLSSGTMRTLYGMILARSIAETGSPERGWEWLRRFDAQTKEYVINPALLMEKL
                     NRREGLVTVWELTDMLWQEKRGAPLGFRFPTSGTPVIDDSIGLVKGAPHPEPAKAFID
                     FIGSTEGQELAAREAFRLPARTDIPPAGLPQWARDVLAEMVPAQVDWALMERQGQEWM
                     NTWDRSIRGKGSRGGGAAPASAASPSGH"
     gene            complement(21126..21386)
                     /locus_tag="VEL74_17555"
     CDS             complement(21126..21386)
                     /locus_tag="VEL74_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011242652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HYG64388.1"
                     /translation="MPEICRFYGIIIRMYFDDHAPPHFHAEYGEREILMDINTLAVIA
                     GKLPPKAMGLVVEWATLHQEELRLTWQRARGYEPLGKIAPLE"
     gene            complement(21379..21663)
                     /locus_tag="VEL74_17560"
     CDS             complement(21379..21663)
                     /locus_tag="VEL74_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HYG64389.1"
                     /translation="MFKPIEVRALPGYKIHVRYTDGVSGEVDLSHLVGKGVFSLWSDP
                     REFARVHIGPAGAIVWNEDVDLCPDSLYMQVTGKKPEDVFPSLALKEVDA"
     gene            21731..>22289
                     /locus_tag="VEL74_17565"
     CDS             21731..>22289
                     /locus_tag="VEL74_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HYG64390.1"
                     /translation="MDRFARYSWFTLAYTIAVILWGAAVRATGSGAGCGSHWPLCNGE
                     VVPRAPGVETLIEFSHRVTSGLALLFVVGLAVAAFRSRPRGHSARRAALWSLFFMLTE
                     AAVGAGLVLFEMVADNQSMARALWMGAHLINTFFLVAAMTLTVHFAGGGAPFRLRGQG
                     SAGGWLALGFAGLLLSGVSGAVAALG"
BASE COUNT         3247 a         7844 c         7958 g         3240 t
ORIGIN      
        1 ccggacatgg tgctgctgcg cctgatcggg gagcggcgtc cggaggccgt gtcgcggctg
       61 tacgaccgcc acgctccgat gctcctcgcc ctcgcccgcc gcatcctcgg cagcacggcg
      121 gacgccgagg aggtgctgca ggaggtcttc cttcacgtct gggaccactg cgggcgctac
      181 gacccggcgc gctcgtcggt ctccacctgg ctggtgctgc tcacccgcag ccgcgccatc
      241 gaccggctgc gccaccgcaa cgtcgtggag cggacgcacg agcaggccca tgcctccggc
      301 acctccgagg gcacggaccc gcgggacgat gcatctcccg gcggccccga cgccgtattg
      361 atcagagaga gacgggaacg ggtccggcgg gagctcgaag gcctcccccc cgagcagcgg
      421 caggttttgg agatggcctt ttacgagggt ctgtcgcaga gcgagatctc cgcccgggcc
      481 gggctccccc tggggacggt caagacgcgg accctgctgg cgatgaagaa gctgagaaac
      541 gcgctacgca tggaaatcag ggagctgctg tgagccagga gaccccggag gatcgcacca
      601 tcctctcggc gctcgacgcg ccggacggcg ggatcggcgt gtccgctcac accggtctgc
      661 ccaagaccgc gcgggacgag accgccgaga ccctggcgcg gctctacacc gaggtgctgg
      721 ggctgctccc cctggagctc gagccgcggg cgccccggcc cgagctcaag gagcgcctga
      781 tggcgtccct ggccgccgtg gcgacaccag cggtggcgga cgaggtccag gctccggcgc
      841 cggccctccc ggccgcggag cctccggtgg cgtctcgaga ggtggccacg gagccggctg
      901 cgccgccggc ggcccctccc gagccccggc cgtcccagga ggtgcggatc ccgcatccgg
      961 cgacgctgcc ggcccctcct ccccccccgc cgcggcgctg gccgctggcg ctggccgccg
     1021 ccctggccct tatctttgcg gggttgtccg gcgttctggg ctacctggtg agcgagcagc
     1081 gggtgcgcat gaaccagttg gaggcggatc tggacaaggc ccgcgatgcc gagacgcggg
     1141 cccgccgggc ggaggcgcag gccgacaggg ctcacgccga catgctgaag atgcgcgaga
     1201 gcctcagcct ggtgaccacc cccagcgtcc tcgtcatgcc cctgcgcccg caaggcgatt
     1261 cggcgcccca gccggacgcc aagggggtcc tcttcgtggc cgccgaccac cagcattggc
     1321 atctcgccct gcaccagctc aggccggccg agccgggacg gcagtaccag ctctggttcg
     1381 ccaccgacga cggccggacc gtgagcggcg gcaccttcac cgtacgcggg ccgacggtga
     1441 ccatgtcgtc cccgacgatg cccacgggga cggtggcggc cagcatcacc gtcgagcccg
     1501 ccggcggctc catctcgccg accgggcccg aagtcctcaa gacgaacgag gccttccagc
     1561 tctcgtagct acttccccgg cggccggctg ggccgcatct ccaccaggct gggcagggcg
     1621 tgcggcgggt acgtgaggag gtggagcacc atggccgcca cgtcctccgg ctgcaggcgc
     1681 cagctccggt cgccggtccg gtccgggtgg ccgaagccgg tgtcgacgct gccggggagg
     1741 atggaggcga cccggatgcc gtgctggcgc aggtcgagca tcgaggcctc ggacatcccc
     1801 accagcccga acttgctggc gttgtaggcc gctcccccgg ccatggcgtt gcggccggcg
     1861 agcgaggcga tgttgaagat ccatcccgag ccctgccgct tcatggccgg cgccaccgcc
     1921 cgggtgaagt agaaggggcc ggagaggttg gtctcgatca cctcccgcca ttgctcgccg
     1981 gtgatctcgt ccaccggatt gaacaggccg accccggcgt tgttgaccag gccgtcgatc
     2041 cgtccctcgc gggcgagcac ccgctcgacg aacccgtcga cctcctcctg gcgccgcgcg
     2101 tcgaccgctt cgccggccac ggcgtccccg aagcgcccgg cgagctcgcg gaccgcctcc
     2161 gccaccgagc ctggcgtcga ggccggcgtg cggctgcaca ggtggacccg ccaaccctcg
     2221 ccgagcagcg cctcgacgat ggcgcggccg atgccgcggc tccctcctgt gaccagggcg
     2281 actttcttct cttccattgg gctcatgcgt ccaccctcct tcttgaggta ctgtatcaac
     2341 cagaccacgc aggaccgggt ctgaaatgtg gcgccccccg gaccttgttc taagggggcg
     2401 aaggagggtg cgagcgaaat gtacttagat cccatctact tcgcgttcat cctgccggcc
     2461 ctggccctgt cgctgtgggc gagctggcgg acgaagtcgg cgttcaacaa atactccaag
     2521 gtgcggacct accggggtat gaccggcgcc caggcggcgc aggagatgct gcgggcggcc
     2581 aacgtccaag gcgtggagat cgtcccgacg cacgggatgt tgaccgacca ctacaacccg
     2641 gtgtccaaga agctggccct gtcggaggcc gtgtatgcct ccaactcggt ggccgcggtg
     2701 ggcgtcgcct gccatgaggc cgggcacgcc atccagcacg cccagaacta catgcccatg
     2761 tggatccggt cgatgctggt gccggtggcg aacatcggct cgacggtggg ctacatcgtc
     2821 atgtccctgg gcctcatgct gatgtacatg agccgtagcg ccgagagccc cggcatgggc
     2881 caggggatcg tcctcttcgg cgccgtgctg ttctcggcgg tgctgctgtt ccagatcgtc
     2941 accctgccgg tggagttcga cgcctcggca cgagccaagc ggctggcggt cgagaccggc
     3001 atcgtcctgc cccaggagcg cgaggggatg gacaaggtgc tgaacgccgc ggcattgacc
     3061 tacgtggccg ccgccgtctc caccctgatg acgttgctct actacctcct ccgtgccggc
     3121 ctgctcggcg gccgtcgcga gtagcccacc tgaatacgcg ccaggtgaga agcgaggggc
     3181 gccccgcccg gagagatccg ggcggggcgt ttccgttcca gggtggtccg ctacaatggg
     3241 cgcccacatg caggaggtga cgatgcgata cctactcatt ctcgtgacag cagctctcct
     3301 gacggtcccg gccgcagctc agacggctcc ggccccgaag actccccccg ccgacgacca
     3361 ttcgtcccac gcccatgccg ctccggcggc cccggcaacc ccggcaaccc cggcaaatac
     3421 cgccgccgcc ccccgcgacg agaacctccc gccgggcgag cagcaggcca aggccgcgct
     3481 ggagaagtct ccccgccacg gcgagctcgt ggacgtgaag ctccccgcgg gcgggacgcc
     3541 gatccgcacc tatgtcgtct accccgagcg caaggagaag gcgggcgtgg tcatcgtcat
     3601 ccacgagatc tacggcctca ccgactggat ccgcggcgtc gccgaccagc tcgcccgcga
     3661 gggcttcatc gccgtggcgc ccgacctgat ctcgggcctg ggtccgaacg gcgggggcac
     3721 cgactcggtg gcgagccgcg acgacgtgac gaagctggtg cgcgggatct ccccggaaga
     3781 cgccaccgcc cgcctcaacg ccgtccgtga ctacgcgatc aagctcccgg ccgccaacgg
     3841 caagagcgcc accgtcggct tctgctgggg aggagcgcgc agcttcgcct atgccgcggc
     3901 gcagccgggc ctgaacgccg ccgtcgtcta ctacggcacc tcgccggaga acgccgccga
     3961 ctacgccagg atcaaggcgc cggtgctggg cctctacggc gaggacgacg cgcgggtgaa
     4021 cgccacgatc ccgccggccg aggccgagat gaagaaggcg gggaagagct acgagccgca
     4081 catctacaag ggggccggcc acggcttcct gcgcgcccag gacggccgcg acggcgccaa
     4141 cctcaaggcg acccagcagg cctggccgcg gacggtggcg ttcctgcggc agcatctgag
     4201 gtaggccgga cggcgctgtc cggcggcggg gccggagggg ttacaatccg cccgctatgg
     4261 cctggtttcg acgcaagttc cggctgaccg cctgcgccac caccggcggc tgagcggcca
     4321 aggtaggtcc gggggacctt tccaccttga ttggaccgct cgtccggctc gccgggcggg
     4381 acgagcgggt cctcgtcggc ccggaaacgg ccgacgatgc cggcgtgtac ctgctcggcg
     4441 accgggccct gatcgccacg accgatttca tcaccccggt ctgcgacgac ccccgccgct
     4501 tcggccgggt ggcggcggcc aactcgctgt cggacgtcta cgccatgggg ggccggccgc
     4561 tcttcgccct gaacctctgc tgcttccccg aggaggtgcc gggcgacgcc ctcgccggca
     4621 tcctggaggg ggcggcggcg gcgctggccg aggcgggggc ggcgctcctc ggcggccact
     4681 cggtgcgcga cccggagctc aagttcggcc tggccgtcac cggcgagggg gatcccaacc
     4741 ggctgctcac caacgccggg gcgcgggcgg gggaccggct cctcctcacc aagcccctcg
     4801 gcaccggcgt gctggtcaac gccttcaagt tcgacaagct ggacgaggcg gggctcgagc
     4861 cggcgctgct ggaaatggag cggctgaacg ccgtcgcctc gcggctcgcc ctggagcacg
     4921 gcgtccacgc ggccacggac atcaccggtt tcggcctcgc cggccacgcc ttcggcatgg
     4981 cccgggcgtc cggggtgggt gtccggatcg ccttcgggcg cctgccggtc tacgacgcct
     5041 tctatcccct ggcgaagagc ggcgtcacca ccggctgcac ccgcgccaac gaggacaacg
     5101 tccaccaggc gttcaccgac cacgccggcc tcgacaaggt gcagcgggac gtcctcttcg
     5161 acccccagac ctcgggcggt ctcctcctct ccacgccgcc ggagcaggcg gcgcccctgc
     5221 tcgcggccct gcgcgagacc ggccaccgcg ccgccgagat cggcgaggtg gtggcgggcc
     5281 cgccgcggct ggaagtggtc tagctcagca gatccttgag cgccttgagc tcgtcggcga
     5341 gctgggcgac ggtcgactcc aggcgctcga cgcgggcggc gagggagggg cggtccggtc
     5401 gctcggcggg gagtccggcg gctcccgcgg tgtaggccgg ctcgggggcg ggctcggcga
     5461 cctcccctac gagatggatc cagcggctct ccttctggcc ggggcggcgg gggagctcac
     5521 ggaccagcgg ctcgtccatg ccggccatcc cctggagcgc cgcctcgacc tcctcgacgc
     5581 tggcaaacgg gtgcaggcgg tcgctgcggg agcggagctc gccgggggtc tgggcaccgc
     5641 gcaggaggag cagggtcatc agcgccttgc ccgcccggtc gaggccccag cggcggacga
     5701 cgctctgctg ccagcgctcg gtgcgcgccc cctcggtgcg ccaggcgagg acgacctggc
     5761 gcaggcggtc cagggcgtcg accacctgct cctcggtcag ctccatcacc gggtcgcggt
     5821 tggtcttctg gttggcgccc gcgaggacgg cgttgacggt cagcgggcag gcctcgggtg
     5881 tggtctgctc cttctcgagg agcacgccga ggacccggat ctccaccggg tcgagctggc
     5941 ggggtgtgcg cgtcgatgtc accggactcc cttccccctc gagccggcac cgaggtgcgt
     6001 ccgagggcag gaagggatct tatccgatcc ctccgtttcc cctcttgact tcccgttccg
     6061 tagttgcgat gctacccgca acttctggtg ttcggtgtgg aaacggttgg aagcagcatc
     6121 acctcagaga tcacccctct agtcgtctct ccttgcgcat acccgctcgc accccaggtt
     6181 taaatagaaa gacatcaatg tttcaggact caggctgggc ccggaggaaa agcgggggac
     6241 atccccccgg tacgcagctc ccatgaagtc gcttcgcgcc cggaggagat agccggagac
     6301 cgcctcacgt cgatcctcgg cgaccttgaa gttttctttt gttgggtttg cctctgtgcc
     6361 gttccggcac cggggtggag gagtgccgct tcggcggcgg aataaacaac tcgttttgtt
     6421 tcaaggagca agcgtatgcc agagcaaggt accgtgaagt ggttcaacga cgccaagggg
     6481 tatggcttca tcgtgcggga gcggggcgcg gacgtcttcg tccatcatgc ctcgatcatc
     6541 gccgagggct tccgtaccct gagcgagggt gaccgggtcg ccttcgacgt cgtcgagggt
     6601 cccaagggcc tgcaggcgaa gaacggcgtc aaggtttcct gacccctcca cttcggtttt
     6661 catagcgtac gccccgccga gatgtgttcc cggtggggcg tcttgcttca ggctgttttc
     6721 agaggacccg gatcagcgga accgcgcgcg gaactgctgg taggccttgg tgtcgcgggt
     6781 gaggttgccc cgccgggaca atccggccgc ctccgcctcg acgttgcgga tgcgcgtctc
     6841 ggtcatcgga tggttcgaga agaactgctc gaccttggtc ggccggcgct ggcgcatgtc
     6901 gaggagctgc cggaagaagt cggccacgcc gtccgggttg tagccggccc ggtaggcgta
     6961 ctgcaccccc agacggtccg cctcgcgctc ggcgtcacgg ccgtaggcgt tgagcgccgt
     7021 cacgccaccc aactgagcga ggagctgctg gaactgcccc gggttgcggc ccaggagcag
     7081 gcccgccacc aggttgtagc cataggcccg ggtcatgagc tgcgtgccgt ggcgcgccgc
     7141 gccgtggccg atctcgtgcg cgagcacgcc ggcgagttgg tccagggtgt ccgcctcggc
     7201 gatcagcccg gtattgacat agaccaggcc acccggcagg ttgaacgcgt tgacgctcgg
     7261 atcgttgacg atgaagaagt cccagggccg gttggcatag ggcgtctggg ccgccagccg
     7321 ccggccgacc gtgttcaggt acgccagcgc ctggcggtcg ttgaccagcc gcgcctcgcg
     7381 ctgcacctgc tgatgcagct cgctgcgcat ctgccactcc tggtccagcg agacgaggtt
     7441 caaaccgcgc ttctgcgcct gcgcgtcagc ggtgcacagg gtcagcgcga ggaacgtcat
     7501 ggcgaccatg gccgcgggcc tggcgaagga agacgtgtga gctttgctaa acatggatgg
     7561 aaacctcctt ccgtccgcgt ccccggaacc tccggggctc gccttctcct tacggcaaag
     7621 tcggtgccag ctcatccccg gatgccaaca tcgctataag atcaaatttt gcagaaggtt
     7681 atagaaggac tccgaggtat tccccgccgg agggcgtcgt ggagggggct ggagtgtcct
     7741 ctccggaggt cgccttctcc cgtggagggg gcatactgga cgtgagccgg tttgtggata
     7801 ccatcacgcg gtcgccgact ccgggacgac cgccgggagg gtctctatgc gccgagccgt
     7861 gatcgccgtc gtttttctgt tcctggcctt tcaggcctgg gggccgcgtc tcgccgaggc
     7921 gagcgaggcg gggggcgggg ggcatggcgg gccagtggtg ccggtgctgc tcggactggt
     7981 gctgatcctc tccgccgcca agctgggggg cgagctgttc gagcggatgg ggcagccggc
     8041 agtcctgggc gagctgatct gcgggatgct gctcggcaac ctggcgctgc tcggcctccc
     8101 gggcttcgat ttccttcaca ccacggccgg gctggagatc ctggcgcaac tcggcgtcat
     8161 cctgctgctg ttccaggtgg ggctcgagtc caacgtcaag gagatgctgt cggtgggctg
     8221 gtcgagcctg ctggtggcgg tgctcggcgt catcgccccc ttcttcctgg gttggggggt
     8281 gtcggcctgg ctgctgccgg aggagccgcg gctggcgcac atcttcatcg gcgccaccct
     8341 ctgcgccacc agcgtcggga tcacggcgcg ggtgctggcc gacctgggca agctggcggc
     8401 gcgggagtcg aagatcgtcc tcggcgcggc ggtcctcgac gacgtgctcg gcctggtcgt
     8461 cctgtcggtg gtctccggca ccatcacggc ggccaacacg ggctcgtcgc tcgaccccat
     8521 ggatgcgggg gccatcatcc tgaagtcgct ggcgttcctg gtgggcgcgg tggccctggg
     8581 cggctggctc tccccccgcg tcttccggct ggcgagccgc ctcaagatcc gggggctgct
     8641 gctggcgatc tccctggcct tctgtttcct cctcgcctac ctggcggacc gcatcggcct
     8701 cgccgccatc gtcggcgcct tcgccgcggg gctgatcctg gacgaagtcc actacaagga
     8761 cttgcgggac cgcggcgagc acaacctgga ggagctgctc catccgatcg ccggcttcct
     8821 ggtgccggtc ttcttcgtcc tcatgggggt gaaggtcgat ctccggagct tcgggcaggt
     8881 ggaggtgctg ggcttcgcgg cgctgctgtc gctcgccgcg gtcctgggca agatgatctg
     8941 cgccgccggc gtgctggagc gggggctcga ccgggtgtcg gtggcggtgg gcatggtgcc
     9001 ccgtggcgag gtcggcctca tcttcgcgag catcggcgcc cagctcgtgc tcgggggcca
     9061 gcgggtgatc acgccgccgg tcttcggcgc cgtggtggtg atggtcatcg tcaccaccct
     9121 gatcaccccc cccgtcctca aggtcaccct cgcccgcggt gaccgcaaga aggccgcggc
     9181 gctgaaaccg cccggtacag acgagccgat ggaccgtccg aacccgtcgc tggggtagga
     9241 aagcgcgcga gcggcaaccc tccgcctgtc agcggcaaag ccgtgccgcc ggcaggccgg
     9301 gcggcgccgg tgggtggccg agcgttgccg gtgtcaggcg aagcggcgcc tgcgacaggc
     9361 cgcacggtgc cggtgacagg ccgggcgagg ccgatgatcg gccgcacggt gcccgtgacc
     9421 ggccaggtgg tgcctgcggc gggccgtgcc gtgccagaga caggcggagc ttcgcctccc
     9481 ctacagggga gcggaggctg cgggagccgg cgacgacgcc gtcaccggcg tggcgacggg
     9541 aggcgtagcg gccgggggtg gaaccggcgt gggggccggc accagcggct cggaccgggg
     9601 ggccgggcgc ggggtgtccg ctccgggggg gccgtcgggc acgatgatga cgaggtcgcc
     9661 cgggcggacc atgccgaggt cctcccgggc gtggcgctcc agggcgccgg gatccttgcg
     9721 cagacggtcg atgcgggcgt gaagccgctc gatgcgcgct tccgtctcgt ggatgcggtt
     9781 ctcgagcagc gcttcacggc cgcgggaggc ctccaggtcc cgcgagctct tgaggctcgc
     9841 cacgccgagg agcaagagaa aaaggaccac cgccgctccc agcacggggc ggaacgaatc
     9901 gagccggcgg gggggcggtt ccgggtgatt caaggggacc agcgtgggaa ggcggccgcc
     9961 ccgggatagg tggcgaccac atccagctcc tccgcgatgc gcagcagacg gttgtacttg
    10021 gcgacccggt cgctgcggca gggcgcgccg gtcttgatct ggccggcgcg ggtggcgacg
    10081 gcgaggtcgg cgatggtggt gtcctcggtc tcacccgagc ggtggctcag gatgttcgcg
    10141 taggcgttgc gcttcgccat gtccaccgct tccagcgtct cggtcagggt gccgatctgg
    10201 ttcaccttga ccagcagcgc gttcgccatc ccctcggcga tccccttggc caggatctcc
    10261 gggttggtga cgaagaggtc gtcgcccacg agctgcacgc ggctgcccag ctcccgggtg
    10321 agctccttcc accccgcgtg gtcgttctcc gccaggccgt cctcgatgga gaccagagga
    10381 taggcgtcga gccattcacg gtagagggcg atgaggtcgg agctggcgag ccccttcttg
    10441 ccctcgccgg ccagggcata tttgacgttc ttgcacccct tgccctccac cagctcgctc
    10501 gcggcgacgt cgagcgccag gccgacctgg cggcccgggg tgtagccggc cttctcgatc
    10561 gcctgcatca acaggtcgag cgcctcgcgg ttgctcttga ggtccggcgc gaagcccccc
    10621 tcgtcgccca ccgcggtgac cagcttgcgg cctttgagca cgtccttgag cgtgtggtag
    10681 atctcgacgc ccgcctgcag cgcctccggg aacgtttcga agcccatggg gacgatcatg
    10741 aactcctgga tgtccaccga gttgtcggcg tgggcgccgc cgttgagcac gttgagcagc
    10801 ggcaccggca gcaccgtcgc cgcggggccc cccagatagg catacggagg caggccgctc
    10861 gcctcggccg ccgccttggc gacggcgagg gagactccca ggagggcgtt cgcccccagc
    10921 cggctcttgt ccggcgtgcc gtcgagctcg atcagcgcgc ggtcgatgcg cgcctgatcg
    10981 cgggcgtcca gcccgcgcat cgtcggcccg atctcgtcca tgacgtgccc cacggccttg
    11041 gacacgcctt tgccacggta gcgcttgcca ccgtcgcgta gctccagcgc ctcgcgctca
    11101 cccgtggacg ctcccgacgg caccgccgcc cgtcccccca cgccgccgtc caggatgcag
    11161 tccacctcca gggtcggatt gccccgcgag tccaggatct cgcgcgcgac gatctcttcg
    11221 atacagaaca ttcaccctcc tctgaatggc aggttgccgc acgactccca gcctaaaggg
    11281 tagccgcaaa gcctcgtctc ctcaaggact ttgatccgaa tcgcccgacg cagcgatgtc
    11341 ggaagcaagg gtggtgccca catggtgccg gtagcggtgc caggaagggg agcgtgctag
    11401 actcgccgcc ccatgttctc gaaggcgcgg cgctccgatt cgtcccaacc catccggctg
    11461 cacatcggca gcggcaaggt gcgccttccg ggatggctca acgtcgacat ccaggctctc
    11521 cccggggtgg atgtcgtggc cgacgtcacc aaggggctcc actacacgga cgtggaggcg
    11581 atcttcgccg agcacttcct ggagcacctg gcgatcgacg acgccctcaa gttcctgcag
    11641 gagagccacc acgcgctcgc cgccggcggc tggatccgcc tgtcgacgcc gaacctcgac
    11701 tgggtctggg tgacgcacta ccggctcgag gcggacgagg agatgaagcg cacctatgcc
    11761 ctggggctca accgggcctt ccacggctgg cgccaccgct tcctctggaa ccgcgagatg
    11821 ctgggcgagg cgctgctcgc ctgcggcttc gaagagcccc gctggtgccg ctacggcgag
    11881 agcggcctcg atctcttcca ggggatcgag caccacgagg tctaccagga cgctccggag
    11941 ctgccgcacg tcatcatcgc cgaggcgcgc aagggggagc cccggccgga gcggctgcgg
    12001 gccttcgaag agcggatccg ggaaggcttc ctgaaccaca tggcggacta ggcgccgccg
    12061 gcggtgtcga cctccacccc tccttcagcc cgcgtcgccc gcatcctggc cgtcgtcgcc
    12121 tccaccgctt tggccgtggc cgcggtctgc gcctggggct ggtaccgtgc cgcccgcgac
    12181 cgggtccccc tggagtcgct gggccccgaa gaccggcagg cgttggtcca ggagatgctc
    12241 gccgcgagcc ccggcgcctt cgcccccgcc ctcttcgagc ccgccatcgg ctacaccctg
    12301 cgcccgggcg aggagatcga ggcctggggg gacaccttca ccagcaacga gctgggctac
    12361 cgcaccgggc cgcccggcaa gccgaagcgg acgttccgcg tcgtcttcat cggggactcc
    12421 tggacgttcg gcctgggggt gagcgaggcc gagtcgttcc ccagccagct cgaagccctg
    12481 gcccgccagg ccggagccgc tggccctggc ggccggcggc aggtcgaggt gcgcaacctg
    12541 gggctgcccg gctacaacac gatgaacgag ctggcggcgc tcgacttctt cttcgaccgc
    12601 ctgcgcccgg acgcggtggt gatctgcccc acggccaacg acgcggacag cgccagcaac
    12661 atcctgccca acggctcgct cacccgcgcc ggcgtggagc gggacggcta cggcgcggac
    12721 cactccatgg tcttccggac gcgcctggcc gactcgtacc tgttccggac ccgctggcgc
    12781 gcggccttcg ccggcatccg cggcctggag gagcggctgc ggcggcgggg ggtgccgctg
    12841 ctggtctact tcgccgcgag ctgggacgag cccttcgccc acgcgctggt ggcggacgcc
    12901 gggctcgacg ccccctatgt ggtgacgccc cccggcttcg ccggcccgcg ctggcgcaac
    12961 ccgcccccct ggcggcacgg cacgcccgag gcgaaccgga tctacgcgcg catggtctac
    13021 aacggcctcg ccgagctcct cggctggccg gcgcagccca aagaggaggg cgccgaggtg
    13081 ccggtctacc gcagggggac ggccggcgac gggtccgccc gggcggagga gctgctgcgc
    13141 gaggagacgc ggcgcatccc cgagagctac gtgccgggcc ccgacgcgga gatccagtgc
    13201 gtcggcgcca tggactggac caccggcctc accggcaagg cgacgacggt gctggtacga
    13261 cgccgcgccg gcgcccgccg ggcggccgtg accctgcgtc gcctgccgga cgccccatcc
    13321 ttatatccct tggccgtgcg ggtctcgatc ccatcggccg ccggggggac cgaggccgaa
    13381 tccactctgc cggcggacgg cccgggcatc acagacctgc agcgcgtcgt gctggacatc
    13441 cccggggacc tccccgaggg cgccgccctg gacatcacca tccgcgccgc ccgggccgcc
    13501 gccgcccccg gcgtgctggc gccgcggtcg atgtacatcg cggggatcga gcaggagtaa
    13561 gggaagtgtg gaaaaacccc cctcctgacc tcccccactc cctaccaccc accacccagg
    13621 gggggaggca ccaccaccct tgctggctgg ggcttatggg cctggccctg gtctttttcg
    13681 gcgcctgcgc cccctctccc cctccccctc agccgcgcaa cgtggtgctg atcctggtgg
    13741 acaccctgcg cgccgaccac ctgggggcct acggctacgg ccggccgacg agcccgaacc
    13801 tcgacgcctt cgcccgcggc gccgtcctct tccaggacgc ccgcagccag gcctcctgca
    13861 ccttcccgtc ggccaactcg ctgctgacct cgcgctaccc cgcggcgttc atgggccagc
    13921 cggagcaggc gatgggcatc cccgggggga tcccctccgt cgccgaggtc ctgcagggga
    13981 agggctaccg gaccgtggcg gtctccgcga gcccgatcgt ccgcaagtcc cccacccggt
    14041 tcaaccccca gggcggcttc gcccggggct tcgagacctt ctccgaggac tgcctgtgga
    14101 agcccgccga gtgcgtgaac cgggtcacga tggagcaatt ggagcagttg gagaaggagg
    14161 aggacggccg ccccttcttc ctttatctcc attacatgga cccccacggc ccctacgcgc
    14221 cgccgccggg ccacgtccgc cgtttcgcga ccgggagccc ggacaaggcg ttcatccgcg
    14281 aggggaaccc gaacccgatc ggcgacatgc tctacaaggg ggcccccgat cccggcgcca
    14341 ctccggccga cctccagcac ctggtcggcc tgtacgacga cgagatcgcc tacttcgacg
    14401 cccagctcgc cctgctcctc cagcggctcc gggagaccgg ccggatggag gacacgctgg
    14461 tcgtcctcac ctcggaccac ggcgaggagt tcctggagca tggccacatc aagcactgcc
    14521 ggacgctctt cgacacctcg atccgcatcc ccctgatcct ccacgtgccc ggcgccgctc
    14581 cccggaccgt gacccggccg gcggagaacg tggacgtcgt ccccaccctg ctggacctcc
    14641 tggggatcgg caccgagggg ctccgtctgg agggccgcag cctgcggccg ctgctggggg
    14701 atgaggagag tgacgagagc ccggaccacc agttcagcgc ccagggcccg ctgcgcagcg
    14761 ttgccgatgg cggcttcaag ctggtggagg acctggccgg ggggagcgtc gccctctacg
    14821 acctgcgggc ggacccgggg gagacgaagg acgtcctgcg cgaccgccgc cgcgacttcc
    14881 atcgcctgcg ccaggcgctg accgcctggc tcgccgccac cgaggggcag cacgccgccg
    14941 gcgagagcgt ccaccaggcc gaggaggcgg agaggaagct gcgcgccctg ggctacatcg
    15001 agtagctccc tggaataccc gccccctcct gccggttatc ctctgtgcgt ccggcggagg
    15061 gtgttgacaa gcgctgggcg gcgtgggatt attcgccctc gcgcccccac aagggccagt
    15121 ttgacaggtt cacggtgacg cggggtggag cagtctggta gctcgttggg ctcataaccc
    15181 aaaggtcgca ggttcaaatc ctgtccccgc aaccaaattt gattttcccg atctttcaaa
    15241 gccgcgtgcg aacgcggctt tttcacttca gggggcgctc cgtcccttgc ctggcggctc
    15301 ccctgcctgg tactcttcgt tcttgtggat accgtagaga tctccctggg actcacctgc
    15361 accgtcaggc gcgacggcga ggatcggtgg gtggcgggct gcccggcact ggacctgttc
    15421 tcccaggggc agagcgagga agaggccaag cgttctctcg aggaagccat cgggctctgg
    15481 gtcgaggact gccttgagcg tggcacgctg gatcaggccc tgcgcgagct cggtttcgac
    15541 agggtgcacc cgggaacgac ggtctctcct ggggacaagc ccattccggc aggcgccgtg
    15601 tcggaagatg cggttccggc gccggctacc ttccccgtcc acatctcgat tcctacgtat
    15661 cgagccgcag cactactggc cagctaaggg caacgcttga agcgcctgag ctggaaggag
    15721 ctgcgggata tctgtttgct tgcaggctat gtcgagtcga ggacccgagg cgaccacctg
    15781 atcatgtctc gcccgggctc cgcccgcccg gtggtcatta agatggatgc cgaccttggc
    15841 gaagacatca tccagagcaa caagagaacc atgggcctca ccaccgcgga gttcgaagct
    15901 ctcctggagc aggtgcgggg gaagaggaag ccccggtgat cctcctgccc cgccgtcagg
    15961 gcttcgagcc ggcgggtagc gggtagggct ccggctcctg cgggcggagc ttcagggtca
    16021 gcgtgccgac gccgagcatc agagcggcgg cgacccagaa cgggtactcg atgccgatct
    16081 ggaagacgac gcccgcccag agcgggccca gcatcttcga gacgccgccg aaggcctgct
    16141 gcacccccag cgtctgcccg gtctcgccgc gggcggagcg gcgcgagacg agggcggtgg
    16201 tggacgggaa gaggagggcg gtcccgacgg ggacgaacag cgccaccacc gtcagcagcg
    16261 tgacccggcc ggcgtagggg aggtccacct gggccggcag cgggatgcag gccaggccga
    16321 cgatcagcga cagggcgccg agccgcacca cccccacctc gccgaagcgg cgcaccgccg
    16381 gccccagcag cagcgcccgc atgatcaccc ccaccgcgcc gacgtagacg aagaaccagc
    16441 cgatggtccg ctcggtgacc ccgaaggcgg cccccaggta gaggggcagg atggtggtca
    16501 tcgagaagaa ggccatcatg ccgatggcat agatccagat cagcgacggc accggtcccg
    16561 acgggtggag gaggacgtcg acgaccgccc gccgcagccg ccggggcacg ccggcctcct
    16621 ggtgccgcgg cggctcgggg agccagcggc tggcgaacag gaagttcatc aggcagagcc
    16681 cggcggcgat gaacccgggc gcctcctggc cgaaggtcga ggcgagcgac cccacggccg
    16741 gcccgatcat cacgccggcg ctggtggcgg cggtgagcca gcccagcccc ttggcgcgct
    16801 cctcggggcc catggagtcg gcgacgtagg cctgcaccac gccgatcgtg ccgccggccg
    16861 ccccctgcac cagccggaag gcgaggagca tccagagcgt ggtcgccagg ccgaagaggg
    16921 cgaaggcgac cgccgagaag accagcccgg cgaggatcat cggccggcgg ccgtagcggt
    16981 cggacatcct cccccagagg ggggcggtgg agaactgggc gaaggcgtag gccgtggtca
    17041 gggcgccgac cacccagggc cgcgccccca gctccgtcgc atagaacggc agcagcggct
    17101 gcaccatggt gaagccgatc atgtcgacga aggccgtcgc catcaggatc cagatctggc
    17161 gcacgtaggc cggcatgctt ctcctactat ctcggccgac gcgcccctag cgcacgtcgc
    17221 cctgccccca cagcaggaag gccacggtgc tcgccaccat gagcagcacg ccgtagaccg
    17281 ccgccatccc cagatcctgg agccgcaggc tggagaggat ctcgatggaa atggggcggg
    17341 tgtcgaaggt gtagagcacg atggagacga cgaagtcccc cagcgcggtg atgaacgcga
    17401 ggccggcgcc ggcggcgagc gccggccgga gcagcggcag ggtgacccgc gtcaggcgcc
    17461 ggagccgccc ggctccgagg gaggacgcgg cctcgtccag cgccgggtcg agcccgcgca
    17521 gccccgccag ggccgcccgg ccggtgagcg gcaggctgcg caccaggtag gcgagcggca
    17581 ggatcagcgg cgtgccgatc agcacgaagc gccccaccgc cggctgctgg acgctgaagg
    17641 tcgtggcgag cgccacggcg aagaccgtcc ccgggatcgc ccacgggagc ccgaggagcc
    17701 cctcgagcgc gccgccgagg gagcccttgc gggccagagc cgcccgcgcc gccgcgaatc
    17761 cgaggacgag ggcggcggcg gtggaggcga gggccatcca cagcgagttg aggaccggcc
    17821 gcaggcgctc ggcgtcggtg aacagggccc ggtagttgcc gaagtcgagg accgggggga
    17881 acggctcggc cgtccaggtg aacgccggca ccagggagac cagcgccagc accgcgtgcg
    17941 gcaagagaag gagggcggcg agcagatagc cgagcgcgcc ggccgtccag cgcgccgccg
    18001 ggctgcgcag ccgccgccgc gtcggcgcga tgccgcgcac gccggtgccg gtgctcgcgg
    18061 cgcggtcggc ccgccgcatc gcccagaggg cgagcagggc gaggccggcc agcatcacgg
    18121 tctccacctg ggccagggcg agctcgccgt tcagccgcga cgccatgatc tgcgtcgtca
    18181 tcacccggaa ggtgccgccg aagaggtagg gggcggagaa cgacgcgagc gaggtcatga
    18241 acgtcagcag ggaagctccg gcgagggccg ggcgcagcaa cggcagcgtc acccggaaga
    18301 aggtccgcac ccgcccggcg ccgagggaag cggccgcttc gagcagcgcg gcgtcctgcc
    18361 gggcgagggc cgcgcgggtg aagagataga agtagacgta catcgagtag gtgtggacca
    18421 ggaggatcgc ccccggcccc accaggcgcc acggcggccc gtcgagcccg aggccggcct
    18481 gcaccgcgcg ggagatgaag ccgctctccc catatagaaa aaggaaggcg atgacgccga
    18541 ccaggggggg cagggcgacg ggcagagcgg ccaccgcgcc caggaagcgg cgccccggga
    18601 actcggtgcg ctcgaagagg aaggccagcg ggacccccac cagcgccgac agcaccaccg
    18661 acgcggcgga gatccagagg ctccgccaga gggccagcca ctcgtccggc cggccggcga
    18721 aggcggcgaa gtggccggtg gtccaggccc gcggcccccc cagcgcctcc atcaaggtga
    18781 tgaggagcgg gtacccgacc agccaggcga ggagcacggc cagcagggcc gggacgagga
    18841 gcctctcacg cgacgacatg gctcaaccaa cgtcccggga cggcccttcc cctctggaag
    18901 aggcccggaa gatccgcggc ttcgggcctg ccccgcccgg cgcgacggcc accgcgtccc
    18961 ccggagcggc ggcgtcggga gcggccatca cctccacctc tctcccggat tccagggcca
    19021 cgacgaagta agtgaccggg ccggcatagc gccgctccgc cacctttccg gggagcgctc
    19081 cggccgtccc gggggaggcg aagcgcagcg actcgggccg gaccaccagc tcggcggggc
    19141 tcccctccgc cagctcctcg gccggaaccc cgggccagga gacgccgccg tccaggacga
    19201 cctgtcctcc ccggaaggtg ccgggcaggg cggcggcgcg gccgacgaag ccggcgacga
    19261 agcgggtggc cggccgctcg tagaggtcct cggggctccc cacctggtgg aggatgccgc
    19321 cctggagcac cgcgacgcgg tcccccaggt cgaacgcctc ctcctgctcg tgggtcacca
    19381 ggacggtggt gatccccacc cgctggatgg cggccttgag ctcgcggcgg gtgcgctcgc
    19441 ggagggtggg gtcgaggttg gagagcggct cgtcgaggag gaggacccgg ggctcggggg
    19501 cgagggcgcg ggcgagggcg acccgctgct gctggccgcc ggagatctcg ccgacgcgcc
    19561 gcccttcgaa gccggggagg tccaccagcg ccagcgcctc ggagacccgg cggcggatgt
    19621 cggcgcggtc cgtcttcccg cgcgactcca ggccgaaggc gacgttctcg ccgacggtca
    19681 ggtgggggaa gagggcatag tgctggaaga ccatgccgaa attacggcgg gcggggggaa
    19741 aagttgtcac gtcctcccct ccgacccgga gatggccggc gtccggcgtc tcgaagccgg
    19801 cgatgatgcg cagggcggtc gtcttgccgc tgccgctggg ccccaggagg gcgaggatct
    19861 gcccctgctc cacggcgacg gacagctcgc ggaggacgcg gttgctgctg aagctcttgt
    19921 cgagccggtc cagctccagg aacggcatgg ctcagtgccc ggatggggag gcagcggagg
    19981 cgggcgcggc gcccccgccc cggctcccct tgccgcggat gctgcggtcc caggtgttca
    20041 tccactcctg gccctggcgc tccatgaggg cccagtccac ctgggccggc accatctcgg
    20101 cgaggacgtc gcgggcccac tgcggcagcc cggccggggg gatgtcggtc cgcgccggca
    20161 gccggaaggc ctcgcgggcg gcgagctcct gcccctcggt gctgccgatg aagtcgatga
    20221 acgccttggc cggctcggga tggggcgccc ccttgaccag gccgatggag tcgtcgatga
    20281 ccggcgtgcc gctggtgggg aagcggaagc ccaggggggc gccgcgcttc tcctgccaga
    20341 gcatgtcggt gagctcccag acggtgacga gcccctcccg gcggttgagc ttctccatga
    20401 ggagggccgg gttgatgacg tactccttgg tctgggcgtc gaagcggcgc agccactccc
    20461 agccgcgctc ggggctgccg gtctcggcga tcgagcgggc gaggatcatg ccgtagaggg
    20521 tacgcatggt gccgctggag agcgggtcgc gcatcaggat cttccccgtc cacttcgggt
    20581 cgaggaggtc gtcccagtcg cgcggcgcct cggcctcggg caccgcctgc ttgttgaaga
    20641 cgaggaccgg tgcggtgcgg taggagccga agtagaggtc ctgggggtgg cggctctcgg
    20701 ggggcacggc gtcggcccac cgcggccggt agggggcgag gagcccctcg cgctccccct
    20761 gggcgaagat ggcccggggg ccgccgtacc agacgtcggc ctgcggattg gcggcctcgg
    20821 agcggacgcg gtcgtagacg tcctgggagc ccatgtcgag ccagcggacg tcgacgtccg
    20881 ggtggctctg ctcgaagctc ttctccatca gggagaggag gtcgcgtcca tggggggagt
    20941 agacgacgag aggcgtacgg ccgtcgccgc atcccgagat caggaggggc aggaagggca
    21001 gggcgagggc gacgccggaa aggaagcggg ttggtttcat ggctcggggt cccgccgaga
    21061 agggggcggg aaggtccgcg gcagtttatc ccggaatggt ccgggagagg gcgagtggag
    21121 gccggctact ccaggggagc gatcttcccg agcggctcgt agccccgagc tctctgccag
    21181 gtgagtcgga gctcctcctg gtgcaacgtc gcccactcca ccacgaggcc catggccttc
    21241 ggcgggagct tgccggcgat caccgccagc gtgttgatgt ccatgaggat ctcccgctcc
    21301 ccatactccg cgtggaagtg tggaggcgcg tgatcgtcga agtacatcct tatgatgatg
    21361 ccgtagaacc ggcagatctc aggcatcgac ctctttcaag gcgagactgg ggaagacatc
    21421 ttccggcttc ttgcccgtca cctgcatgta aaggctgtcg gggcagagat ccacgtcttc
    21481 gttccagacg atcgctcccg ccgggccgat gtgcacgcga gcgaactccc gcggatcgga
    21541 ccacagggag aacacaccct tgcccaccag atgagacagg tcgacctcgc cggagacgcc
    21601 gtcggtatac cgaacatgga tcttatagcc gggcaaggct ctgacttcga tgggtttgaa
    21661 catgctcgac acctcttcgg ggagaattta ccacgtccgc ctctccgctc cctcacgcta
    21721 tgatctcacc atggaccgct tcgcccgcta ctcctggttc accctggcct acaccatcgc
    21781 cgtcatcctg tggggggccg cggtgcgggc cacgggatcg ggggcggggt gcggcagtca
    21841 ctggccgttg tgcaacggcg aggtggtgcc gcgggcgccg ggggtggaga ccctcatcga
    21901 gttctcccac cgggtgacca gcggcctggc gctcctcttc gtcgtcgggc tggcggtggc
    21961 cgccttccgt agccggcccc gggggcactc cgcgcgcagg gcggcgctct ggtcgctgtt
    22021 cttcatgctg accgaagcgg ccgtgggggc cgggctggtc ctgttcgaga tggtggcgga
    22081 caaccagtcc atggcgcggg cgctctggat gggcgcccac ctgatcaaca ccttcttcct
    22141 cgtggcggcc atgacgctga ccgtccactt cgccggcggc ggcgccccct tccggctgcg
    22201 cggccagggg agcgcggggg gatggctggc gctgggcttc gccgggctgc tgctgtcggg
    22261 cgtcagcggc gcggtggcgg ccctcgggg
//
LOCUS       DASPJI010000625         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_210008, whole genome shotgun sequence.
ACCESSION   DASPJI010000625 DASPJI010000000
VERSION     DASPJI010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210008"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VEL74_17570"
     CDS             complement(<1..276)
                     /locus_tag="VEL74_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64391.1"
                     /translation="MDLTGINRRPEDLFALSDMLLQGSQQETFPYDVEILGKPFAVFA
                     NVFSPKHLPGAETFFPMLPSTEGIRLLEVGTGIGAVAVLAALAGARRV"
     gene            complement(339..2015)
                     /locus_tag="VEL74_17575"
     CDS             complement(339..2015)
                     /locus_tag="VEL74_17575"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HYG64392.1"
                     /translation="MDREGAVIDVALPPAQTEAAVAAWRGHARRLLDALGWSGEELAV
                     RPFSGGVSLALSAPVDALYSATEINEWAWRAAAAELAGSPPPDLREGTALLRDAIERE
                     ENPALQALQAAAAARRVTLLADDDQVSVGLGKGSRTWPARSLPDPDTIDWSKIHDVPV
                     ALVTGTNGKSTTVRLLAAMARSGGWTVGTTSTDRIEVGDEVLDRGDYSGPGGARTLLR
                     DRRVEMAILETARGGILRRGLALRTADAALVTNVAEDHLGDYGVLDLDGLAETKMVAP
                     RAVRPGGRVVLNADDPQLVGRVAGLAAAITWFSLDPANPTVRAYLVAGGTACLLDEAD
                     LLLARGERRLRIARAEEVPIAFGGAARYNLANALAAMGLADALGLPAVAMAAGLRRFR
                     PDPESNPGRANLMELGSIRVLVDYAHNPHGLAAVLAFAASLPAARRLVVLGQAGDRDD
                     EAIRELARTAWSFRPDHVVLKELPSLLRGRPLGEISRVLADEMRRLGAPAEAVSRAED
                     DMDATRQALAWARPGDLLVLLTHTLREDVLALLGKLRREGWEAGAVVASS"
     gene            2149..>3001
                     /locus_tag="VEL74_17580"
     CDS             2149..>3001
                     /locus_tag="VEL74_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016858998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HYG64393.1"
                     /translation="MVSTSSSANPFARCWALDPDVSFLNHGSFGACPRAVLEEREALY
                     RTLERQPVEFLVRRLAERFDAARSALAVFLGADPEDLVAVANATSGVNTVLRSLSFSP
                     GDELLTTDHAYNACRNALEHTAERWGARVVVARVPFPLESPAQVEEAVLSAVTPHTRL
                     ALLDHVTSPTGLVFPVESLVPRLAERGVDSVVDGAHAPGMLPLHLGALGAAYYTGNCH
                     KWLCAPKGAGFLHVRRDLQTTIRPLVISHGANRPRPGRSRFHDEFDWMGTGDPTPFLS
                     VPAALRVM"
BASE COUNT          396 a         1107 c         1084 g          414 t
ORIGIN      
        1 cacccgccgc gcgccggcca gcgccgcgag gaccgccacc gcgccgatcc ctgtccctac
       61 ctccaggagg cggattccct cagtggacgg gagcatcggg aagaaggtct ccgccccggg
      121 caggtgcttg ggagagaaga cgttggcgaa gacggcgaag ggcttcccca ggatctccac
      181 gtcatagggg aacgtctcct gctggctccc ctggaggagc atgtccgaga gtgcgaagag
      241 atcctcggga cgtcggttga taccggtcag atccatctgg agcctcctgc catccaccag
      301 cttcccgtag gtacggggat ggcgcctcca agttttcttc aggacgaagc gacgaccgct
      361 ccagcctccc aaccctcccg ccggagcttg ccgaggagcg ccaggacgtc ctcccggagt
      421 gtgtgggtga ggagcaccag gaggtcgccc ggccgggccc aggccagagc ttggcgggtg
      481 gcgtccatgt cgtcctcggc gcggctcacg gcctccgccg gagcgcccag ccggcgcatc
      541 tcgtccgcga ggacgcggga gatctcgccg aggggccggc cgcgcagcag gctgggcagc
      601 tccttgagca cgacgtggtc cggccggaag gaccaggcgg tgcgcgccag ctcccggatc
      661 gcctcgtcgt cgcggtcgcc ggcctgcccg aggacgacca ggcggcgcgc ggcgggaagc
      721 gaggcggcga aggcgaggac tgccgccagg ccgtgcgggt tgtgggcgta gtccaccagc
      781 accctgatgc tccccagctc catgagattg gcccgccccg ggttgctctc cgggtcgggc
      841 cggaagcggc gcaggcccgc ggccatcgcc acggcgggca gccccagcgc gtcggccaag
      901 cccatggcgg ccagggcgtt ggccaggttg tagcgggcgg cgccgccgaa ggcgatgggg
      961 acctcctcgg cgcgggcgat gcgcagccgg cgctcgccgc gcgccagcag caggtccgcc
     1021 tcgtcgagca ggcaggcggt gccgccggcc accagatagg cgcgcaccgt cgggttcgcc
     1081 ggatcgaggc tgaaccaggt gatggcggct gccaggccgg cgacgcgccc cacgagctgc
     1141 gggtcgtccg cgttgagcac cacccggccg cccggccgga cggcgcgcgg ggccaccatc
     1201 ttggtctccg cgaggccgtc gaggtcgagg acgccgtagt cgcccaggtg gtcctcggcg
     1261 acgttggtga ccagggcggc gtcggcggtg cgcagggcga ggcctcggcg caggatgccg
     1321 ccgcgcgccg tctccaggat ggccatctcc acccgccggt cgcgcagcag ggtgcgggcg
     1381 ccgcccgggc cggagtagtc gccgcggtcg agcacctcgt cccccacctc gatgcggtcg
     1441 gtggaggtgg tgcccaccgt ccagccgccc gaccgggcca tggcggccag caggcggacg
     1501 gtggtggact tgccgttggt gccggtcacc agggcgaccg gcacgtcgtg gatcttgctc
     1561 cagtcgatgg tgtcagggtc gggcagggag cgcgccggcc aggtacggga gcccttgccg
     1621 agccccacgg agacctgatc gtcatcggcg agcagggtga cgcggcgggc ggcggcggcg
     1681 gcctgcaggg cttgcagcgc cgggttctcc tcgcgctcga tggcgtcgcg cagcagggcg
     1741 gtgccctcgc gcaagtcggg gggggggcta ccggcgagct cggccgcggc agcccgccag
     1801 gcccactcgt tgatctcggt ggcactgtag agggcgtcga ccggcgcgct cagcgcgagg
     1861 ctgacgccgc cggagaaggg gcgcacggcc agctcctcgc cgctccagcc gagagcgtcg
     1921 agcagccggc gggcgtgacc ccgccacgcc gcgacggccg cctcggtctg tgccggcggc
     1981 agcgccacgt cgatcactgc gccttcccgg tccaagagca gaccaggtcc tgtcaacctg
     2041 cgagagtcga ggatctccat ccggatctcc ggaatacggg ggttttcgag caagagcgta
     2101 tcagatgaat tggcactgcg agcacccggg ttcggataat ctccctccat ggtctccacc
     2161 agctcctcgg caaacccctt cgcccgctgc tgggcgctcg atccggacgt ctccttcctc
     2221 aaccacggct ccttcggcgc ctgcccccgc gcggtcctcg aggaacggga ggccctgtac
     2281 cggacactgg aacgtcagcc ggtcgagttc ctcgtccgcc gcctggccga gcggttcgac
     2341 gcggcccggt ccgccctcgc cgtcttcctc ggcgccgacc ccgaagacct ggtggcggtc
     2401 gccaatgcca cctccggggt gaacacggtg ctccgctcgc tctccttctc cccgggggac
     2461 gagctgctga ccaccgacca cgcctacaac gcctgccgca acgccctgga gcacacggcg
     2521 gagcggtggg gcgcccgggt ggtggtggcg cgggtgccgt tccctctgga gagccccgct
     2581 caggtggagg aggcggtcct gtcggcggtc accccgcaca cccggctcgc cctgctggac
     2641 cacgtcacca gccccaccgg cctggtcttc cccgtcgagt cgctggtgcc gcgcctcgcc
     2701 gagcgcggcg tcgactccgt ggtcgatggc gcccacgctc ccggcatgct ccccctgcac
     2761 ctcggcgccc tgggggcggc gtactacacc ggcaactgcc acaagtggct gtgcgcgccc
     2821 aagggggcgg gcttcctgca cgtccgccgc gacctccaga ccaccatccg gccgctggtg
     2881 atcagccatg gcgccaaccg tccgcgcccg ggccgctcgc gcttccacga cgagttcgac
     2941 tggatgggca ccggcgatcc cacccctttc ctctccgtgc cggcggccct gcgggtcatg
     3001 g
//
LOCUS       DASPJI010000626         6746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_553166, whole genome shotgun sequence.
ACCESSION   DASPJI010000626 DASPJI010000000
VERSION     DASPJI010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553166"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VEL74_17585"
     CDS             <1..674
                     /locus_tag="VEL74_17585"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYG64394.1"
                     /translation="IFLDEVGEMTPSLQAKMLRVLEAGEYSRVGSTAIRRSDARVVAA
                     THRDLGAMVREGRFREDLFYRLDVVEVTLPPLRERRGDIPLLTRYFLEQHNRKAGKDR
                     QLSAAAEERLLAHDYPGNVRELQNALQRAVLLANGPLIEPQDLPAPFRAAAVRGSDGE
                     PLGGFRAAKQRVIEEFERDYLTRCLREAGGNISQAARAAEIDFKNFHIKMTQYGIDPA
                     AFKKR"
     gene            complement(671..3589)
                     /locus_tag="VEL74_17590"
     CDS             complement(671..3589)
                     /locus_tag="VEL74_17590"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYG64395.1"
                     /translation="MHGTLTRQGREALLNDEPLAGQPPPGRILSPGAVLAGRFRVMAL
                     LGEGAMGEVYEAEDLELGERVAVKVLRPEIAWDERVLKRFKREIQLARKVTHPHVCRV
                     FDLFFHRFGGEKESGAALAFVTMELLAGETLEERLARSGRMSAAEIVPLVRQMAEGLE
                     AAHRAGVVHRDFKSGNVMLTPAPDGSPGGLRAMVTDFGLAWSSSGSASLTTTGVMVGS
                     PAYMAPEQVRGEEVTAATDIYALGVVLYEMATGSLPFSAETAFGTAIKRLREPPTLPR
                     RHAPDLDPRLESVILRCLESDPGERFATPGEVAAALEQAAAPATERRRRHVRWRAVAV
                     AAALVAAAGLAVWVVATERRSAGSPGDIRPAGAVRSFDSSEALPENAYIGESLYAEGL
                     ARRARFDPKGARQLLEQARDADPDNPLIRAELAVTLAALGYDAQAREEGAEAFERAAA
                     LPSGKRRLVEARYREVTRDWELAAEIYQDLWASSPTDFEHGLFLARVQIQAGQHDKAL
                     ETVERLRATPSPSPDPRTDLVEAEAALRQNDVERGMIAATRAVTVATAQKAVLIAARG
                     LHFQGIALRLSGRLDDARQTSERAARLFREAGDPVSEADALNNVGGSLLTQGEVEGAR
                     RTFERVVEIGGEVGSSTVRAMGLTNLGGFLLQTGDLEGARARYDQAAVAYREAGKRDL
                     EAQVLNNLGLINLRLGRRAEARAMFSEVVRVFQELGNPGREGVATENLGHFALEEGNL
                     REARRHFTRALQLHRRVNNFRGIALSQAGLAQALFLIGDLPGARRELEMGITTLESLG
                     ASPLAAQNRLVLARFLVDSGDLDGGEAMARQAETRLTAGGQPDDVVLAQTILAAVALE
                     RGKAGEALAAMERLAAPLAKCQNDGIRRYAAVIAALARAEQDPRGAAQRLTELAEHAE
                     QSGLRPAALEIRLALGEIEMDGLDPAAGRVRVAAVQRAARAAGLGGLLRQAAEALRKP
                     "
     gene            3710..>6746
                     /locus_tag="VEL74_17595"
     CDS             3710..>6746
                     /locus_tag="VEL74_17595"
                     /inference="COORDINATES: protein motif:HMM:NF024593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4011 domain-containing protein"
                     /protein_id="HYG64396.1"
                     /translation="MTGPLDPQTPSDVSTPEGQRVTAALARWKRKLLDLSKRNRALNF
                     RPYKVSTVAIVDEQPAEVFRQLYLLERSLRFKPAPEPAKGTPRETATEIAEPDELEVQ
                     GSSLDFTAYDASRLDPRHTDDVLQTASPLEQLQKSLRRLEEQARSAIEEQGVNPLFLA
                     LGMLHYKREKEEDVLRAPLVLVPVTLGRRSVEAGYTLHAADDDPIVNPALAEYLRQRG
                     IVLPELPDLTDIAADYDLQTLFGAASAAIAEQAGWTLKTDVFLGLFSFQKFVIYKDLE
                     ANGPSFASHRLIRRLVTRGGPKSMETVVGLPGEVRELDLDAGFPPEATAQVVDADSSQ
                     LRAMAAVQREHDMVLEGPPGTGKSQTITNLIAQALAAGKSVLFVAEKMAALQVVHGRL
                     VGAGLGEFCLELHSSRANKRTVMRELASALDASLQRPQMPEPSGPRLQTVRRELDGYV
                     EAVHTPWGALGYTPYRAYGELGQVLNAPKLRLTAPVAQVTREQLTDAARDLRSLAELA
                     RDVDDPTAHPWRETTRTFYSEDDLDNLATLLADLAARLEALLQRADEVHQAFALPPVR
                     TFAGTETAAVIAAALGRSPGVSLEILRSDGWNAPPPQALEIAARGRRVAQLRERLGGR
                     LAPEALERDHADDATYIERKEAGALRFLNVLSGRFREIRRRWTGWRRPGWQPTLIEQA
                     AEMRQVDALRHERQALTGEDPAARQLFGALWQGEASSWDALDAYIRWVVEVRALALQH
                     RLDERALEIAARPQPDLSTVEALRREAAEAAGRLAGLRTAVGWPADHLAASPFGEIME
                     RVQAMADHLRLAPRWAAFESVRARAAAGPGAEVVAAALRGEVPFPDLAAAFLRAFYQR
                     WLSAVVQEREPLRAFHALAHEQRVEEFRRLDQQVLQENRARLVRELRDRTQKRLQSPE
                     ALAGMPTLKREMVKQRALAPLRKTLRNAETAIRSIKPCFMMSPLTVAQLLDGKAPNFD
                     LVVFDEASQLPAEDAVGAIVRGRQLVVVGDPKQLPPTDFF"
BASE COUNT          942 a         2529 c         2345 g          930 t
ORIGIN      
        1 cgatcttcct cgacgaggtg ggcgagatga ccccgagcct gcaggccaag atgctgcgcg
       61 tcctcgaggc cggcgagtac tcccgggtgg gcagcaccgc catccgccgc tcggacgccc
      121 gcgtcgtggc cgccacccac cgcgacctcg gcgccatggt gcgcgaaggg aggttccggg
      181 aggacctctt ctatcgtctc gacgtggtag aggtcaccct gccgccgcta cgcgagcgcc
      241 ggggggacat ccccctcctc acccggtatt tcctggagca gcacaaccgc aaggccggca
      301 aggaccggca gctctcggcc gcggccgagg agcggctgct cgcccacgac tacccgggga
      361 acgtccgcga gctgcagaac gccctccagc gggcggtgct gctggcgaac gggccattga
      421 tcgagcccca ggacctgccc gccccgttcc gcgccgcggc cgtgcgggga tcggacggcg
      481 agccgctcgg cggcttccgc gccgccaaac agcgggtgat cgaggagttc gagcgcgact
      541 acctgacgcg ctgcctgcgc gaggccgggg gcaacatcag ccaggcggcc cgcgccgcgg
      601 agatcgactt caagaacttc cacatcaaga tgacgcagta cgggatcgac ccggcagcgt
      661 tcaagaagcg ctagggtttt cggagcgcct ccgccgcctg gcgcaggagc ccgccgaggc
      721 cggcggccct ggccgcccgc tgcacggcgg cgacgcgcac gcggccggcg gcgggatcga
      781 ggccgtccat ctcgatctcg ccgagggcga ggcggatttc cagggcggcg gggcgaaggc
      841 cgctctgctc cgcatgctcg gccagctccg tcagacgctg cgcggcgccg cgggggtcct
      901 gctcggcgcg ggccagcgcc gcgatcaccg cggcgtagcg ccggatgccg tcgttttggc
      961 acttcgccag cggcgccgcc agacgctcca tggccgccag cgcctccccg gccttccctc
     1021 gctccagggc gacggcggcg aggatggtct gcgccagcac cacgtcgtcg ggctgcccgc
     1081 cggcggtgag ccgggtttcg gcctggcgcg ccatggcctc gccgccgtcg aggtcgccgg
     1141 agtcgacgag gaaacgggcg agcaccaggc ggttctgggc cgccaggggg gaggccccca
     1201 gagactccag cgtggtgatc cccatctcca gctcccggcg cgccccgggc agatcgccga
     1261 tcaggaagag ggcctgggcc agcccggcct gactcaacgc gatcccccgg aagttgttga
     1321 cccgacggtg gagctggagg gcgcgggtga agtgcctccg cgcctcgcgc aggttcccct
     1381 cctccagggc gaagtgccca aggttctccg tcgccacccc ctctctgccg gggttcccga
     1441 gctcctggaa gacgcggacg acctcgctga acatggcccg cgcctcggcc cggcgcccga
     1501 gccgcaggtt gatcagaccc aggttgttca ggacctgcgc ctccaggtcg cgcttgccgg
     1561 cctcccggta ggccaccgcc gcctggtcgt agcgagcacg ggccccttcc aggtccccgg
     1621 tctgcaggag gaatcctccg aggttggtca ggcccatcgc ccggacggtg ctgctgccga
     1681 cctcgccgcc gatctccacc acccgctcga aggtgcggcg cgcgccctcg acctccccct
     1741 gagtcagcag gcttccgccg acgttgttga gggcgtccgc ctcggagacg gggtcgccgg
     1801 cctcccggaa gaggcgggcg gcccgctcgc tggtctgccg ggcgtcgtcg agccttcctg
     1861 acagacgcag ggcgatccct tgaaagtgga ggccgcgggc ggcgatcagg accgccttct
     1921 gggccgtggc cacggtcacc gcccgggtcg ccgcgatcat cccgcgctcc acgtcgttct
     1981 ggcgcagggc ggcttcggcc tccacgagat cggtccgcgg gtcgggggat ggcgacggcg
     2041 tggcccgcag gcgctcgacg gtctccaagg ccttgtcgtg ctgccccgcc tggatctgga
     2101 ccctggccag aaagaggcca tgctcgaagt cggtgggcga ggaggcccag aggtcctgat
     2161 agatctcggc cgcgagctcc cagtcgcggg tcacctcgcg ataccgcgcc tccaccagcc
     2221 gccgcttccc ggaaggaagc gcggcggccc gctcgaaagc ttcggccccc tcctcgcgtg
     2281 cctgagcgtc atagccgaga gcggccaggg tgaccgccag ctccgcccgg atcagtggat
     2341 tgtccggatc ggcgtccctc gcctgctcca ggagctgcct cgctcccttc ggatcgaaac
     2401 gtgcgcgccg cgccagcccc tcggcataga ggctctcgcc gatgtaggcg ttctccggca
     2461 gggcttcgga gctgtcgaag ctccgcaccg ccccggccgg acggatgtcg ccgggactcc
     2521 ccgcggaccg tcgctcggtc gccaccaccc agacagcgag gccggctgcg gctaccaggg
     2581 cagcggccac ggcgacggcg cgccagcgca cgtggcggcg gcgcctctcg gtcgcggggg
     2641 cagccgcctg ctccagggcc gccgcgacct cgccgggcgt ggcgaagcgc tccccgggat
     2701 cgctctccag gcagcgcagg atgacggact ccaggcgcgg gtcgaggtcc ggagcgtggc
     2761 ggcgcggcag ggtcggcggc tcgcgcaggc gcttgatggc ggtgccgaag gcggtctcgg
     2821 cgctgaacgg caggctgccg gtggccatct catagaggac gacgcccagg gcatagatgt
     2881 cggtggccgc cgtcacctcc tcgccgcgca cctgctcggg ggccatgtag gcgggcgatc
     2941 ccaccatgac gccggtggtg gtgagggacg ccgaaccgga gctgctccag gccagaccga
     3001 agtcggtgac catggcgcgc agcccccctg gcgatccatc cggcgccgga gtgagcatca
     3061 cgttgccact cttgaagtcg cggtggacga cgccggcgcg gtgggcggcc tccagcccct
     3121 cggccatctg gcggaccagg gggacgatct cggcggcgct catccggccg ctacgggcga
     3181 ggcgctcctc cagggtctcg ccggcgagaa gctccatggt gacgaaggcg agggcggcgc
     3241 cgctctcctt ctctccgccg aagcgatgga agaagaggtc gaagacgcgg cagacgtggg
     3301 ggtgggtgac cttgcgggcg agctggatct cccgcttgaa gcgcttgagg acccgctcgt
     3361 cccaggcgat ctcgggccgc agcaccttga ccgccacccg ctcgcccagc tcgaggtctt
     3421 cggcttcgta gacctccccc atggcgcctt cgcccagcag cgccatgacc cggaaccgtc
     3481 ccgcgaggac cgcgcccggt gagaggatgc ggcccggcgg aggctggccg gccagaggct
     3541 cgtcgttcag aagcgcctcc ctcccttgcc tggtcagtgt tccatgcaat tgtacacctc
     3601 cccttcactc gtggccctga tacttcaccg gcgaggcgtt gccgcaggat gttacagaag
     3661 gctgcgcgtt tgacatcgcc gggggggagc ggctactttg aggctccctg tgacaggacc
     3721 gctcgacccg cagactccct ccgacgtctc cactcccgaa ggccagcggg tcaccgccgc
     3781 cctcgcccgg tggaagcgca agctcctcga cctcagcaag cgcaaccgcg ccctcaactt
     3841 cagaccctac aaggtctcca cggtcgcgat cgtggacgag cagccggccg aggtgttccg
     3901 gcagctctac cttctggaga ggagcctgcg gttcaagccg gcaccggagc ccgccaaggg
     3961 gacaccccgg gagacggcca ccgagatcgc cgagcccgat gagctggagg tccagggctc
     4021 gtccctcgac ttcaccgcct acgacgcttc ccggctcgac ccgcggcaca ccgacgacgt
     4081 cctccagacc gcctccccct tggagcagct ccagaaatcg ctgcggcgtc tcgaggagca
     4141 ggcgcggagc gccatcgagg agcagggggt caatcccctg ttcctggcgc tgggcatgct
     4201 ccactacaag cgggagaagg aggaggatgt gctgcgggcg ccgctcgtcc tcgtccccgt
     4261 gacgctcggc cggcggtcgg tcgaagcggg ctataccctc cacgcggcgg acgacgaccc
     4321 gatcgtcaac ccggcgctcg ccgaatacct gcggcagcgc ggcatcgtcc tcccggagct
     4381 ccccgacctc accgacatcg ccgcggacta cgacctgcag accctgttcg gcgccgcctc
     4441 ggctgcgatc gccgagcagg ccggctggac cctcaagacc gacgtcttcc tcggcctctt
     4501 ctccttccag aagttcgtca tatacaagga cctggaggcc aacggcccct cgttcgcctc
     4561 gcaccgcctg atccgccggc tggtgacgcg cggcgggccc aaatccatgg agacagtggt
     4621 ggggctgccc ggcgaagtac gggagctgga cctggacgcc ggcttccctc cggaggccac
     4681 cgcccaggtg gtggacgccg actccagcca gctccgcgcc atggccgcgg tccagcgcga
     4741 gcacgacatg gtgctggagg ggccgccggg gaccggcaag tcgcagacca tcaccaactt
     4801 gatcgcccag gccctggccg ccggcaagtc cgtgctcttc gtcgccgaga agatggccgc
     4861 gctccaggtg gtgcacggcc ggctggtagg cgccggcctc ggcgagttct gtctggagct
     4921 ccactccagc cgggccaaca agcggaccgt gatgcgggag ctggcctccg ccctggacgc
     4981 ctcgctccag cgcccgcaga tgcccgagcc ctccggtccc cgtctccaga cagtccgccg
     5041 cgagctggac ggctacgtgg aggcggtcca caccccctgg ggcgccctcg gctacacccc
     5101 gtatcgcgcc tacggcgagt tgggccaggt cctgaacgcc cccaagctgc ggctgaccgc
     5161 gccggtcgcc caggtcaccc gcgagcagct caccgatgcc gcccgcgatc tccgctccct
     5221 cgccgagctg gccagggacg tggacgatcc cacggctcat ccgtggaggg agacgacgcg
     5281 gaccttctac tccgaggacg acctggacaa cctcgccacc ctgctggccg acctcgcggc
     5341 gaggctcgag gccctcctcc aacgcgccga cgaggtccac caggccttcg ccctgccgcc
     5401 ggtgcggacc ttcgccggca ccgagaccgc cgcggtcatc gccgccgccc tcggccgctc
     5461 gcccggcgtc tcgctggaga ttctgcgcag cgacggctgg aacgcgccgc ccccccaggc
     5521 cttggagatc gcggcgcgcg gccggcgcgt cgcccagctc cgcgagcggc tcggaggccg
     5581 gctggcgccc gaggccctgg agcgggacca cgcggacgac gccacctata tagaaaggaa
     5641 ggaggcgggc gccctccgct tcctcaacgt cctctccggc cgcttccggg agatccgccg
     5701 gcgctggacc ggctggcgcc gtccggggtg gcagcctacc ctcatcgagc aggccgccga
     5761 gatgcgccag gtggacgccc tccgccacga gcgccaggcc ctcaccggag aggatccggc
     5821 ggcaaggcag ctcttcggcg ccctctggca gggggaggcc tcctcgtggg acgcgctgga
     5881 cgcctacatc cgctgggtgg tggaggtccg cgcgctcgcc ctccagcatc gcctcgacga
     5941 gcgggccctg gagatcgccg ctcgtcccca gccggacctc tcgacggtcg aggccctgcg
     6001 gcgcgaggcc gcggaggcgg cgggccggct cgccgggctg aggacggcgg tgggctggcc
     6061 ggcggaccac ctcgcggcct cccccttcgg cgagatcatg gagcgcgtcc aggcgatggc
     6121 ggaccacctg cgcctggcgc cgcgctgggc ggcgttcgag tccgtccgcg cccgggccgc
     6181 cgcgggtccg ggggccgagg tggtggccgc cgccctgcgc ggcgaggtcc ccttccccga
     6241 cctcgccgcg gccttcctgc gcgccttcta tcaaaggtgg ctgagcgcgg tcgtccagga
     6301 gcgcgagccg ttgcgggcct tccatgccct ggcgcacgag cagcgcgtcg aggagttccg
     6361 gcgactcgac cagcaggtgc tccaggagaa ccgcgcgcgg ctcgtgcgcg agttgcggga
     6421 ccggacgcag aaaaggttgc aatcccccga ggcgctggcg ggcatgccca cgctcaagcg
     6481 cgagatggtc aagcagcggg cgctcgcccc gctccgcaag accctgcgca acgccgagac
     6541 ggcgatccga tcgatcaagc cttgcttcat gatgagcccg ctcaccgtcg cccagctcct
     6601 cgacggcaag gcgccgaact tcgatctcgt ggtcttcgac gaggcgagcc agctccccgc
     6661 cgaggacgcg gtcggagcga tcgtccgcgg ccggcagctc gtggtcgtgg gcgatcccaa
     6721 gcagctcccg ccgaccgatt tcttct
//
LOCUS       DASPJI010000627         4411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_387430, whole genome shotgun sequence.
ACCESSION   DASPJI010000627 DASPJI010000000
VERSION     DASPJI010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387430"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4411
                     /locus_tag="VEL74_17600"
     CDS             <1..>4411
                     /locus_tag="VEL74_17600"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64397.1"
                     /translation="EPLSPPAVHHAFAAQARRAPDAMALSWPEGGLTYGELDRRAGRL
                     ARSLRRRGLIPGERAILLLERSPELAVAQLAVWKAGGVAVPLDPAWPRDRVERLKEES
                     GAVLVVDGELSIEEDGPPPEDLSTSADAAYVIFTSGSTGRPKGVVVEHGSLAFTLAAA
                     QAVLGWDPMDVTSWSSAPTFDISLLELWAPLLAGGVCEMLPAGPGTDLAAWRELLPRL
                     TRLHAVPGLMRPLIDLVRDEAPPCAGLRTLLVGGEAVPAGLLADLRRTFPNADLGVLY
                     GPTEATIVATVWRVPSPEVPVRSLLGRPLPGVEIQVRDAAGRIVPANVVGEIWIGGPG
                     VARGYLNREQETEERFQKRSYRTGDLARWTAAGELEFRGRADNQVKIRGVRIEPGEVE
                     AALSAAPGVREAAVTVRDDLPGGRGLVAYVAGEGAADALRSFLADRLPAYMVPAAFVL
                     LDRLPRTAHGKVDRRALPAPNAAGLDDAGRVAPRTPLESRLAAMWQEVIGLETEVGAH
                     DNFFSLGGDSIKGAIFTNRLGRQLGEHVPVALIFEAPTVAELAAALEAEYPAALGLSL
                     PGPLSHPLPRTGRGDKGEGAPLSFAQERLWFLQQLDPQGATYNMGDALVLRGRLDTAA
                     LEAAWREILRRHQTLRARFLLRDGLPVAEVGPVPDDPLPRIDLAGLGTAALDAAQRLA
                     HEEAVRPFDLQRGPVVRGLLLRLGPDHHALLFTTHHIVCDGWSLGIWAREIQIVYTAF
                     AAGGPRLPEPPMQYADFAAWQRSWLEGSRLEAQVSYWRERLRGAAPYLELPLDHPRPA
                     FQSHRGGAASLSLPAVLADRLRALSSRHDASLFMTLLAAFSLHLGRLSGQDDILVGSP
                     IAGRIQPEVEGLIGCFLNTLVLRADLSGEPSFRDLLRRVRETALGAFSHQDVPFEKVL
                     EELRPERDTSRPSLFQVLFNLVNVPRAAMDLPGLALENLGPGEPFAKFDLTLYISEED
                     GLALHLVYNSDLFDPPRMEEMLRQYRLLLEQATEHPESPVASYSLLTSEAAAVLPNPV
                     ASLDHPWPGAVHDLFADQARHAPDRVALIDRDGLWTYGELDRAVHRLAARLLADGLGP
                     GDLVAVWAHRGAALAWALLGIVKAGAAFTILDPAYPTPRLIEVLRIARPRGLLRIDGA
                     GPLPPDLEVFVEEQGVVVSPVTGEGGGFFSTGPHDVASVAFTSGSTGLPKGIVQTHGS
                     MSYFLPWHREALGYSESDRHTMLSGLAHDPLQRDIFYSLATGATLCVPDPGRIGEPGY
                     LAAWMAEQRVTVANLTPAMALLLTELQPGAATLMPDLRVTVLAGDLLTRELVERLRAL
                     APRTRFLNVYGTTESHRALSYHLVEAGEDARRRRLKQTLPLGRGQTGCQFLVLTPSGS
                     LAGIGEIGEVAVRSPWLARGYLGDEALTRERFGINPFTGDPGDRLYRTGDLGRYLPDG
                     EVEAAGRADQQVKIRGFRVEPGEVEAELLRAPGV"
BASE COUNT          548 a         1618 c         1560 g          685 t
ORIGIN      
        1 gagcccctgt cgcccccggc cgtccaccac gccttcgcgg cacaggcccg gcgggcaccc
       61 gacgccatgg ccctctcctg gccggaaggt gggctgacct acggtgagct ggaccgccgc
      121 gccgggcggc tggctcggag cctgcggcgg cgcggtctga tcccagggga gcgcgcgatc
      181 ctcctcctgg agcgctcgcc ggagctcgcc gtcgcccaac tcgcggtctg gaaggctgga
      241 ggcgtcgccg tgccgctcga tccggcctgg ccacgcgacc gggtagaaag actgaaggag
      301 gagagcggcg cggttctggt ggtggacggg gagctatcta tagaggaaga cggcccgccg
      361 ccggaggatc tctccacgtc cgcggatgcc gcctacgtga tcttcacctc gggatcgacc
      421 ggacgtccca agggcgtggt cgtcgagcac ggcagcctgg cgttcaccct ggccgcggcc
      481 caggcggtgc tgggctggga ccccatggac gtcacgagct ggtcgtcggc gccgacgttc
      541 gacatctccc tcctcgagct gtgggcgccg cttctcgccg gcggcgtctg cgagatgctg
      601 ccggccggcc cggggacgga tctggcggct tggcgggagc tgctgccgcg gctcacccgg
      661 ctccacgccg tgcccgggct gatgcgtccg ctgatcgacc tggttcgcga cgaggcgccg
      721 ccctgtgccg ggctgcggac cctcctcgtc ggcggcgaag ccgtgcccgc cggtttgctt
      781 gctgacctcc gccgtacgtt tccgaacgcc gacctcggcg tcctctacgg acctacggag
      841 gcgacgatcg tcgccacggt ctggagagtg ccgtcgccgg aggtgccggt ccgttcgctg
      901 ctcggccggc cgctgcccgg cgtcgagatc caggtccgcg acgccgccgg ccgcatcgtg
      961 ccggcgaatg tggtgggtga gatctggatc ggtggccccg gagtggcccg tgggtacctg
     1021 aaccgggaac aggagacgga ggaacgtttt caaaagcggt cctaccggac cggcgatctg
     1081 gcccgctgga cggccgcggg cgagttggag ttccgcggcc gtgccgacaa tcaggtgaag
     1141 atccgcggcg tccgcatcga gccgggagag gtggaggccg ccttgtccgc cgctcctgga
     1201 gtccgcgagg ccgcggtcac ggtccgcgac gacctgcccg gcgggcgcgg gctcgtggcc
     1261 tatgtagcgg gagagggggc cgccgatgcc ctgcggtcct tccttgccga ccgtctgcct
     1321 gcctacatgg tgcccgccgc cttcgttctc cttgatcgcc tgccgcggac ggcgcatggc
     1381 aaggtggacc gccgggccct gccggctccg aacgctgccg gccttgatga cgcgggccgg
     1441 gttgcacccc gcacgccgct ggagtcgagg ctcgccgcca tgtggcagga ggtcatcggc
     1501 ctggaaaccg aggtcggagc tcacgacaat ttcttcagcc ttgggggcga ctccatcaag
     1561 ggggcgatct tcaccaaccg cctggggcgc cagctcgggg agcacgtgcc ggtggcgctg
     1621 atcttcgagg cgcccaccgt ggccgagctg gcggcagccc tggaggcgga gtacccggcg
     1681 gcgttggggc tctcacttcc tggccccctc tcccatcccc ttccccgaac cgggagaggg
     1741 gacaagggtg agggggctcc tctctccttc gcccaggagc ggctctggtt cctccagcag
     1801 ctcgatccac aaggcgccac ctacaatatg ggggatgcgc tcgtcctgcg ggggaggctc
     1861 gatacggcgg cactggaggc cgcctggcgc gagattctgc gccggcacca gacgctccgc
     1921 gctcgtttcc tgctccgtga cggcctgccc gtcgcggagg tggggccggt gccagacgat
     1981 cccctacctc ggatcgacct tgctggcctg gggacggcgg ccctggacgc ggcccaacgt
     2041 ctggctcatg aggaggccgt gcggccgttc gacctccagc gcgggccggt ggtacgcggc
     2101 ctgctgctcc gcctcgggcc ggaccaccat gccctgctgt tcaccaccca ccacatcgtc
     2161 tgcgacggct ggtcgctcgg catctgggcg cgggagatcc agatcgtcta caccgccttc
     2221 gccgccggcg ggccccggct ccctgagccg ccgatgcagt acgccgattt cgcggcctgg
     2281 cagcggagtt ggctcgaggg ctcccggctc gaggcccagg tgtcctactg gcgcgagcgc
     2341 ctgcgcggcg cggcccccta cctggagctg ccgctcgacc acccccgtcc cgctttccag
     2401 agccaccggg gcggtgcggc gtcgctctcc ctgccggcgg tgctggccga ccggctgcgc
     2461 gcgctgagca gccggcacga cgcgtcgctg ttcatgacgc tgctcgcggc cttctccctc
     2521 cacctgggcc ggctgagcgg ccaggacgac atcctggtcg gctcgcccat cgccgggcgg
     2581 atccagcccg aggtcgaagg gctgatcggc tgcttcctca acaccctcgt cctgcgtgcc
     2641 gacctgtcgg gcgagccgag cttccgggac ctgctgcggc gggtgcggga gaccgccctg
     2701 ggtgcctttt cgcaccagga cgtgccgttc gagaaggtgc tggaggagct gcggccggag
     2761 cgcgacacct cgcggccgtc gctgttccag gtcctgttca acctggtcaa cgtgccgcgc
     2821 gccgccatgg acctgcccgg gctcgccctg gagaacctcg gcccgggcga gcccttcgcc
     2881 aagttcgatc tcaccctcta catctcggag gaggacggcc tggcgctcca tctggtctac
     2941 aactccgacc tcttcgaccc gccgcgcatg gaggagatgc tccgccagta ccggcttctc
     3001 ctcgaacagg ccaccgagca cccggaatcc ccggtggcct cctactcgct cctgacctcc
     3061 gaagcggcgg cggtcctgcc caatcccgtg gcgagcctcg accacccctg gcccggcgcg
     3121 gtgcacgacc tcttcgcgga ccaggctcgg catgcgcctg atcgggtcgc cctgatcgac
     3181 cgggacggac tctggaccta cggcgagctg gaccgggcgg tccaccgcct agccgcccgc
     3241 cttctggcgg acggcctcgg tcccggggac ctcgtcgccg tctgggccca ccgaggcgcg
     3301 gcgctggcct gggcgctcct ggggatcgtc aaggccggcg cggcgttcac gatcctcgat
     3361 ccggcctacc cgacaccgcg gctgatcgag gtcttgagga tcgccaggcc gcgcggtctc
     3421 ctccggatcg acggcgccgg tccgctgcct cccgacctcg aggtcttcgt cgaggagcag
     3481 ggggtggtgg tatctcccgt cacaggtgaa ggaggggggt tcttctcgac cgggccgcac
     3541 gacgtggcat ccgtggcctt cacctcgggc tccaccgggc tgccgaaggg gatcgtccag
     3601 acccacggct cgatgtccta cttcctgccc tggcaccggg aggcgctggg ctattcggag
     3661 tccgaccgcc acacgatgct gtcggggctg gcgcacgacc cgctgcagcg cgacatcttc
     3721 tatagcctgg cgaccggcgc caccctctgc gtaccggacc ccggccgcat cggcgagccc
     3781 ggctacctgg ccgcctggat ggcggagcag cgggtcacgg tcgccaatct cacccccgcc
     3841 atggccctgc tcctcaccga gctgcagccc ggcgcggcca cgctcatgcc cgacctgcgg
     3901 gtgaccgtcc tggccggtga cctcctcacc cgcgagctgg tggagcggct ccgggccctg
     3961 gcgccgcgga cgaggttcct gaacgtctac ggcaccaccg aatcccaccg cgccctgagc
     4021 tatcacctcg tggaggccgg cgaggacgcc cgccgccggc gcctgaagca gacgctgccg
     4081 ctcgggcgcg ggcagacggg gtgccagttc ctcgtcctga ccccgtccgg cagcctggcc
     4141 ggtatcggtg agatagggga ggtggcggtc cgcagcccct ggctcgcccg gggctatctc
     4201 ggcgacgagg ccctgacccg ggagaggttc gggatcaacc ccttcactgg agatcccggc
     4261 gaccgcctct accgcacggg ggacctgggg cgctacctgc cggacggcga ggtcgaggcg
     4321 gcggggcggg ccgaccagca ggtgaagatc cgcggcttcc gggtagagcc gggcgaggtg
     4381 gaggccgagc tgctccgcgc gcccggggtc c
//
LOCUS       DASPJI010000628         7456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_672699, whole genome shotgun sequence.
ACCESSION   DASPJI010000628 DASPJI010000000
VERSION     DASPJI010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672699"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1960
                     /locus_tag="VEL74_17605"
     CDS             <1..1960
                     /locus_tag="VEL74_17605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG64398.1"
                     /translation="EGGRLAGLSGIGVPLAAVDHFGLDLAERTHPLILALAGGEPVAF
                     HDSGHALHRPFETPLGAGSFHAVPLARGGGDLAPGLLLVTGPIDGPVEEDVRWAAEML
                     GMRLVSLWYRRAQMDERRHKRERSWLFGIINAVTDPILLTDADGRIVVANAAAERLLT
                     ADEQTSEGRRRAVALNNMLFSASLFTTTEEETSRRELLLVDPTEGQDLLFEVLSTRAK
                     VRAGESGVVSVLRNVTDLRRATEEIEENYRRLRTAEAQTRAERDRLDLILNSVLDPIL
                     VTDAAGNIVLMNPPAERVFTVGPADSVEAERRVRANDAVFTSFVSNLYAGQSLRWRGE
                     LNLLDPRTGDTVPVEAISGKVLSKHGEDTAIVTILHDLTEAVEKARLYEQVKRHSEEL
                     REKVREATGELAEQNELLRRQAFQLEQASAAKSQFLANVSHELRTPLNAILGYTHLLL
                     EGVSGELAPRQTEKLQRVDSNARHLLAIINDLLDIARIESGKMPIQLERFELHELIDE
                     VMAEVEPLITGTRLEVTRAVSPGVPEIRSDRQKVKQIVLNLLSNALKFTPQGSVAIQV
                     GFEEPSEHVFIAVTDTGIGISEENQKTIFEAFGQADSSYTKRHSGTGLGLSICRRLAT
                     LLGGDISLRSQLGEGSTFTLVFPRRPKK"
     gene            1972..2385
                     /locus_tag="VEL74_17610"
     CDS             1972..2385
                     /locus_tag="VEL74_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG64399.1"
                     /translation="MSSEPDMGAEQPPLVLVVDDVAHGREICAEYLEFRGFRVATAED
                     GEEALDKAFELRPDVILMDLSLPRLDGWEATRRLKQDERTREIPVIALTAHALASAHE
                     KAKLVGCDAVVTKPCLPKDLEAEVRRQLEARGERS"
     gene            2382..3311
                     /locus_tag="VEL74_17615"
     CDS             2382..3311
                     /locus_tag="VEL74_17615"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HYG64400.1"
                     /translation="MSDGRKAKAGDKPAKKAAPARKAAAPKLGSAAKDAPASTPAALA
                     PTTPVAVPPPANERSGKYVYCIIQTTEPMRFGPIGIGSEPADVHTVNFQDIAAVVSDT
                     PLEVYDPTRDNVLAHERVNEAVMRTHTVIPMSFGTVFKTSEDIVELLRTAYDAFRDVL
                     IKMQDKLEFGLKVLWEPEIIIREIEKDDENLRLLRQEISHQKGSTYFARMQYGRLVDS
                     LLQERSEQLVAEIFAALAGVSVASRANKPIGDKMILNAAFLVPRELESDFDAKVKEID
                     ARYANLIFKYTGPWPPYNFVNIRLKLERASEAE"
     gene            3308..3643
                     /locus_tag="VEL74_17620"
     CDS             3308..3643
                     /locus_tag="VEL74_17620"
                     /inference="COORDINATES: protein motif:HMM:NF016976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpG"
                     /protein_id="HYG64401.1"
                     /translation="MIILDDLLIGGLRFVLDKVASAVDAEMNDADRLREDLLAAQMQL
                     ELGEITDEEFAEVESEVLVRMRELREQRGGAQAAGAVSLAGGSFGVDVTFGGDDSEGG
                     SRPPRPPGE"
     gene            complement(3602..6565)
                     /locus_tag="VEL74_17625"
     CDS             complement(3602..6565)
                     /locus_tag="VEL74_17625"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYG64402.1"
                     /translation="MSTSPASPPAAGFLARLAGLAQSRPDEPAMAFVSRGEEDQVLSW
                     SELAAQVGRLGRWLRERGVAPGDVVLVLTPSPREQALGFLAAMAAGGVPSILSFPSVK
                     QSEGCFFTMLGSVARNSRARWIVGAPELTGPAGSHPALAGLDVQGIDLPDPAALTGVP
                     LPLEPAPEMSTARPLFLQYSSGTTGARKGIAVTEEMMAFQASSYSQALGMGPDDRVVS
                     WVPLYHDNGLVGCFLLPLYLGAFSAHLQPFEWVADPVSFFQAAARYRATLLWSPNFAF
                     SFCARRIAPEELAGLDLGSLRAFLSGGELVREASRREFLERFGAAGLEERHLQVCYGM
                     AENTFIATQTELGRPVRSDTVDRKTFLAESRAVPLFETAAEAPPEAGITFFSCGRPIP
                     GHEVRIAGGLADRRVGEVEIRSGCLFTGYVPADAAAREAAREVLTPDGWYRSGDLGYL
                     ADGELFVTGREGDMIIHRGVNLHPEDLQEVAGAVPGCKDGRAAVFGVRDEVEGTEQIV
                     ALLEPEDGTDREALARRVRDEVAATFGIGLWDVRVCDPGTLVKSTSGKLSRSGNRRLY
                     LEEMGGRPGRGPRQDAGHVPPRDPWETELVAIWREVLEVPSIGVTDDLFLELGASSLS
                     ALSAVGEIRQRLGRDVEPHQLLGKETIERQAVLLRQEPGEGSAALVPLRGGGPGHPLY
                     LIHPAGGSPFSYLALTRGLGPGQPVYAFRDPHLSSDPGGGPSFASIEEMAAAYLAELE
                     AFDSHGPYALGGWSMGGLVAFEMARRLAERGRPVSLLLMLDTFPPQSALSRAYYAAGQ
                     GLSRALLHTFGAGRAFELLRGGRRMSPGEKLFQMAYVDSGCADPRVMEATFPGLCDPA
                     ELRRLTPDEMWEHVYRRLQEEGLARVVPGRTARVIRREQRLLRLHHQISSRYRPVRQP
                     VRITYFTVRGSRSPRAWRRYSAQPLDVHEFALAPTAGLADAHLSMMQEANVALYAVTL
                     RSLLAGGAGGTGTTL"
     gene            6631..>7456
                     /locus_tag="VEL74_17630"
     CDS             6631..>7456
                     /locus_tag="VEL74_17630"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HYG64403.1"
                     /translation="MTSSPLPSFWFFGGKGGVGKTTCAAAAALAAAEAAGGERRVLVV
                     STDPAHSLGDALAARLGPEPARIPTRRGTLLAAELGAERALKRWKAGHREGLRTLAEH
                     GTYLDERDVDLLLDLSLPGLDELVGLLELSRLARESACDEVVVDTAPTGHTLRLLAVP
                     ETLRHLAAALNEMASRHRLIAESFGGRPLPDAGDALIHEIETEGLDLSGRLRDPERAT
                     FVWVLLPEALALAESRDALAALRAAGIHVQELLVNRLTPPSATCPVCLERRRAELEI"
BASE COUNT         1122 a         2708 c         2654 g          972 t
ORIGIN      
        1 cgaggggggc cggctcgcgg gcctctccgg gatcggcgtc ccgctcgccg cggtggacca
       61 cttcggcctc gacctcgccg agcgcacgca tcccctgatc ctcgccctgg cgggcggcga
      121 gccagtggcg ttccacgatt cggggcacgc gctccaccgg ccgttcgaga cccccctcgg
      181 cgccggctcc ttccacgccg tccccctcgc ccgcggcggc ggcgacctcg ctccggggtt
      241 gctgctggtc accgggccga tcgacgggcc ggtggaggag gacgtgcgct gggccgccga
      301 gatgctcggc atgcgcctgg tctccctctg gtaccgccgg gcgcagatgg acgagcggcg
      361 ccacaagcgg gagcggagct ggctgttcgg gatcatcaac gcggtcaccg accccatcct
      421 gctgaccgac gccgacggcc gcatcgtggt ggccaacgcc gcggccgagc ggctgctgac
      481 cgccgacgag cagaccagcg aggggcgccg ccgcgcggtg gctctcaaca acatgctctt
      541 ctcggcctcg ctcttcacca ccacggagga ggagacctcc cgccgcgagc tgctgctggt
      601 cgacccgacc gaggggcagg acctgctctt cgaggtcctc tcgacccgcg ccaaggtgag
      661 ggccggcgag agcggtgtgg tctcggtgct gcgcaacgtc accgacctgc gccgggcgac
      721 cgaggagatc gaggagaact accgccggct gcgcaccgcc gaggcccaga cccgcgccga
      781 gcgcgaccgc ctggacctga tcctcaactc ggtgctcgac ccgatcctgg tgaccgacgc
      841 cgccggcaac atcgtcctga tgaacccgcc ggccgagcgc gtgttcaccg tcggcccggc
      901 cgattcggtc gaggcggagc ggcgggtacg ggccaacgac gcggtcttca cctcgttcgt
      961 ctccaacctc tacgccggcc agtccctgcg ctggcgcggc gagctcaacc tcctcgaccc
     1021 gcgcaccggc gacacggtgc cggtggaggc gatctccggc aaggtgctgt ccaagcacgg
     1081 cgaggacacg gcgatcgtca ccatcctcca cgacctgacc gaggcggtcg agaaggcccg
     1141 cctctacgag caggtcaagc gccactccga ggagctgcgg gagaaggtgc gggaggccac
     1201 gggcgagctg gccgagcaga acgagctgct gcgccgccag gccttccagc tcgagcaggc
     1261 gtcggcggcc aagtcgcagt tcctggccaa cgtctcgcac gagctgcgga cgccgctcaa
     1321 cgccatcctg ggctacaccc atctgctcct cgaaggggtc tcgggcgagc tcgcgccgcg
     1381 gcagacggag aagctgcagc gcgtcgactc caacgcccgt cacctgctgg ccatcatcaa
     1441 cgacctgctg gacatcgccc gcatcgagtc gggcaagatg cccatccaac tggagcgctt
     1501 cgagctgcac gagctgatcg acgaggtgat ggcggaggtc gagccgctga tcaccggcac
     1561 gcgcctcgag gtgacccgcg ccgtatcgcc gggggtgccc gagatccgct ccgaccgcca
     1621 gaaggtcaag cagatcgtgc tgaacctgct gtccaacgcc ctcaagttca cccctcaagg
     1681 gtcggtggcg atccaggtgg gcttcgaaga gccgtccgag catgtcttca tcgcggtgac
     1741 cgacacgggg atcggcatct cggaggagaa ccagaagacg atcttcgagg cgttcggcca
     1801 ggcggacagc tcctatacca agaggcacag cggcaccggc ctgggcctct ccatctgccg
     1861 ccgcctggcc accctgctgg ggggcgacat cagcctgcgg agccaactgg gcgaagggtc
     1921 caccttcacc ctggtcttcc cccgccggcc gaagaaatga ccgaggagat catgagcagc
     1981 gaaccggaca tgggagcgga gcagccaccc ctcgtgctgg tggtcgacga cgtcgcccac
     2041 ggccgggaga tctgcgcgga gtacctcgag ttccgcggtt ttcgcgtcgc gaccgcggaa
     2101 gacggcgagg aggcgctgga caaggccttc gagctgcgcc cggacgtcat cctgatggac
     2161 ctctcgctgc cgcggctgga cggctgggag gccacccgcc gcctcaagca ggacgagcgc
     2221 acccgcgaga tccccgtcat cgcgctgacc gcgcacgccc tcgccagcgc ccacgagaag
     2281 gcgaagctgg tggggtgcga cgccgtggtc accaaaccgt gcctgccgaa ggatctcgag
     2341 gccgaggtcc ggcggcagct cgaagcccgc ggggaacgtt catgagcgac ggacgcaagg
     2401 cgaaggccgg cgacaagccg gccaagaagg cggcgccggc ccggaaggcc gcggcgccca
     2461 agctgggctc ggccgccaag gatgccccgg cttccacgcc cgccgcgctc gccccgacga
     2521 cgccggtggc ggtgccgccg ccggcgaacg agcgcagcgg caagtacgtt tactgcatca
     2581 tccagacgac ggagccgatg cgcttcggcc ccatcggcat cggctccgag ccggcggacg
     2641 tccacacggt caacttccag gacatcgcgg cggtggtctc ggacaccccg ctcgaggtct
     2701 acgacccgac gcgcgacaac gtcctcgccc acgagcgggt caacgaggcc gtgatgcgca
     2761 cccacacggt catcccgatg tccttcggca cggtgttcaa gacctcggag gacatcgtcg
     2821 agctgctgcg cacggcctac gacgctttcc gcgacgtgct catcaagatg caggacaagc
     2881 tggagttcgg cctcaaggtc ctgtgggagc cggagatcat catccgcgag atcgagaagg
     2941 acgacgagaa cctgcggctg ctgcgccagg agatctcgca ccagaagggg tcgacctact
     3001 tcgcccgcat gcagtacggg cgcctggtgg actccctcct ccaggaacgc tccgagcagc
     3061 tcgtggcgga gatcttcgcc gccctggccg gggtgtcggt ggcgtcgcgg gccaacaagc
     3121 cgatcggcga caagatgatc ctgaacgccg ccttcctggt gccgcgggag ctggagagcg
     3181 acttcgacgc caaggtcaaa gagatcgacg cccggtacgc caacctgatc ttcaagtaca
     3241 cgggcccctg gccgccctac aacttcgtca acatccggct caagctcgag cgcgcctcgg
     3301 aagccgaatg atcatcctgg acgacctgct gatcggcggc ctccgtttcg tgctggacaa
     3361 ggtcgcctcg gcggtcgacg cggagatgaa cgacgccgac cgcctgcggg aggacctgct
     3421 ggcggcccag atgcagctcg agctcggcga gatcaccgac gaagagttcg ccgaggtgga
     3481 gagcgaggtc ctcgtccgga tgcgcgagct gcgggagcag cgcggcggcg cccaggccgc
     3541 gggcgccgtc tctctcgccg gcggctcctt cggcgtcgac gtcaccttcg gcggcgacga
     3601 ctcagagggt ggttcccgtc ccccccgccc ccccggcgag tagcgagcgg agcgtcaccg
     3661 cgtagagggc gacgttggcc tcctgcatca tggacaggtg cgcgtccgcc agcccggccg
     3721 tgggcgccag cgcgaactcg tggacgtcca gcggctgcgc cgagtaccgg cgccaggccc
     3781 gcggcgaccg gctcccccgg acggtgaagt aggtgatccg cacgggctgc cggaccggac
     3841 ggtagcggga gctgatctgg tggtggagcc gcagcagccg ctgctcccgg cggatgactc
     3901 tggcggtccg tcccgggacc acccgcgcca gcccctcctc ctgcagccgc cggtagacgt
     3961 gctcccacat ctcgtccgga gtcagccgcc gcagctccgc cgggtcgcag aggccgggga
     4021 aggtggcctc catcacccgc gggtcggcgc agccggaatc gacataggcc atctgaaaga
     4081 gcttctcgcc ggggctcatc cggcggccgc cgcggaggag ctcgaaggcc cgcccggcac
     4141 cgaaagtgtg gagcagggcc cgcgagaggc cctgcccggc ggcgtagtag gcccggctga
     4201 gggcgctctg gggggggaag gtgtcgagca tcaggagcag ggagaccggc cggccgcgtt
     4261 cggcgagccg gcgggccatc tcgaacgcca ccagcccccc catggaccag ccgcccaggg
     4321 cgtagggccc atgggagtcg aacgcctcca gctccgccag ataggccgcg gccatctcct
     4381 cgatgctggc gaagctcgga ccgccgccgg ggtcgctgga gaggtgggga tcgcggaagg
     4441 cgtagaccgg ctggccgggg ccgaggccgc gggtgagggc gaggtagctg aacgggcttc
     4501 cccccgccgg gtggatgagg tagagggggt gccccgggcc gccgccgcgc agggggacga
     4561 gcgccgcgga gccctccccg ggctcctgcc tcagcaggac ggcctggcgt tcgatggtct
     4621 ccttgccgag gagctggtgg ggctccacgt cccggcccag ccgctggcgg atctccccga
     4681 cggccgagag cgccgagagc gagctcgccc ccagctccag gaagaggtcg tcggtgactc
     4741 cgatcgacgg gacctccagg acctcccgcc agatcgcgac gagctccgtc tcccagggat
     4801 cgcggggagg gacgtgcccg gcgtcctgcc gggggccgcg accgggccgg ccgcccatct
     4861 cctccaggta gagccggcgg ttgccactgc gggagagctt tccggaggtg ctcttgacca
     4921 gggtgccggg gtcgcagacc cgcacgtccc agaggccgat gccgaaggtg gcggccactt
     4981 cgtcgcggac gcggcgggcc agcgcctcgc ggtcggtgcc gtcctcgggc tcgagaagcg
     5041 ccacgatctg ctcggtcccc tccacctcgt cgcgcacgcc gaagacggcg gcccggccgt
     5101 ccttgcagcc ggggacggcg cccgccacct cctggagatc ctcggggtgg aggttcaccc
     5161 cgcggtggat gatcatgtcg ccctcccggc cggtgacgaa cagctcgccg tccgcgagat
     5221 agcccaggtc gccgctgcgg taccagccgt cgggggtgag cacctcgcgg gcggcctcgc
     5281 gggcggccgc gtccgccggc acgtagccgg tgaacaggca gccgctgcgg atctccacct
     5341 cgcccacccg gcggtccgcg aggccgcccg cgatgcggac ctcgtggccc gggatcggcc
     5401 ggccgcagga gaagaaggtg atacccgcct cgggcggcgc ctccgccgcc gtctcgaata
     5461 gggggacggc gcggctctcg gccaggaagg tcttgcggtc caccgtgtcg gagcgcaccg
     5521 gccggcccag ctccgtctgg gtggcgatga aggtgttctc cgccatgccg tagcagacct
     5581 ggaggtggcg ctcctcgagg ccggcggcgc cgaaccgctc caggaactcg cggcggctgg
     5641 cctcgcgcac cagctcgccg ccggagagga aggcgcgcag ggagcccagg tccaggccgg
     5701 cgagctcttc tggagcgatc cgccgggcgc agaagctgaa ggcgaagttg ggtgaccaga
     5761 ggagggtcgc ccggtagcgc gccgcggcct ggaagaagga gaccgggtcc gcgacccatt
     5821 cgaacggttg gaggtgggcc gagaaggcgc ccaagtagag ggggaggagg aaacagccca
     5881 ccaggccgtt gtcgtggtag aggggcaccc agctcaccac ccggtcgtcc ggccccatcc
     5941 ccagagcctg gctgtaggag gaggcctgga aggccatcat ctcctcggtg acggcgatcc
     6001 ccttgcgcgc tccggtggtg ccggaggagt actggaggaa cagcgggcgc gccgtgctca
     6061 tctccggggc gggctccagg ggcaagggca cgccggtgag ggcggccggg tccggcaggt
     6121 cgatcccctg gacgtccagc ccggcgagcg cggggtggga gccggcgggg ccggtcagct
     6181 ccggggcgcc gacgatccag cgcgcccggc tgttgcgcgc caccgagccg agcatggtga
     6241 agaagcaccc ttcgctctgc ttgaccgagg ggaacgacag gatcgacggc acgccgccgg
     6301 ccgccatggc ggcgaggaag ccgagcgcct gctcccgggg agagggggtc agcacgagca
     6361 ccacgtcgcc gggagcgacg ccccgctcgc gcagccagcg ccccagccgt ccgacctgcg
     6421 cggccaactc ggaccacgac agcacctggt cctcctcgcc gcgggagacg aacgccatcg
     6481 ccggctcgtc cggccggctc tgggcgaggc cggccagcct ggcgaggaag cctgcggccg
     6541 gtggagatgc ggggctcgtg ctcatggtcg gtccctctgc cggtccggct gggcgatctt
     6601 agcagggcca tcctatagtg atggccggcc atgacctctt ctcccctccc ttccttctgg
     6661 ttcttcggcg gcaagggcgg cgtcggcaag actacctgcg cggcggcggc cgccctcgcc
     6721 gcggccgagg cggccggcgg cgagcggcgc gtcctggtgg tctccaccga cccggcccac
     6781 tcgctggggg atgccctggc ggcgcggctc ggccccgagc cggcgcggat ccccacccgg
     6841 cgcggtacgc tcctggcagc cgagctggga gccgagcgcg ccctcaaacg ctggaaggcg
     6901 ggccaccggg agggcctgcg cacgctggcc gagcacggca cctacctcga cgagcgggac
     6961 gtcgacctcc tcctggacct ctccctgccc ggcctcgacg agctcgtcgg tctcctcgag
     7021 ctgtcccgac tcgcccgcga gagcgcctgt gacgaggtgg tcgtcgacac cgcccccacc
     7081 ggccacaccc tgcgcctcct ggcggtgccc gagaccctgc gccacctggc cgcggccctg
     7141 aacgagatgg cgagccgcca ccgtctcatc gccgagagct tcggcggccg tcccctgccc
     7201 gacgccggtg acgcgctcat ccacgagatc gagaccgaag gtctcgatct gagcggccgc
     7261 cttcgcgacc cggagcgcgc cacctttgtc tgggtcctgc tgcccgaggc cctggctctc
     7321 gccgagagcc gcgacgcgct cgccgcgctc cgggccgccg gcatccacgt ccaggagctc
     7381 ctggtcaacc gcctgacacc cccctccgcc acctgccccg tctgcctgga gcgccggcgc
     7441 gccgagctcg agatca
//
LOCUS       DASPJI010000629         3257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_553515, whole genome shotgun sequence.
ACCESSION   DASPJI010000629 DASPJI010000000
VERSION     DASPJI010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553515"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VEL74_17635"
     CDS             complement(<1..431)
                     /locus_tag="VEL74_17635"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYG64404.1"
                     /translation="MRRRAIHLWSAAALLGLAGLLLPAIPGAQPRAASQRFPNVLIVT
                     VDTLRADRLSSYGYRRPTSPAIDKLLASGARFTQARTVEPLTNPALCSMFTSTYPHEN
                     GATRNGLRMRRGLPSAAATLGKRGYRTAAFVGNWTLRDEISG"
     gene            476..1333
                     /locus_tag="VEL74_17640"
     CDS             476..1333
                     /locus_tag="VEL74_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014031546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HYG64405.1"
                     /translation="MRPETQPGPAPAVEAEAAARSGEPPRWGKGTESPDDGRFLAGPQ
                     SRAAELVRLWRISADFIRGFRALHFVGPCVTVFGSARFPEDHRYYALARQMGRRLGRA
                     GFTTMTGGGPGVMEAANRGARDVGGRSVGCNIVLPQEQSPNPYLDLWVEFRYFFVRKV
                     MLAKYSYAFVAMPGGFGTMDELFECATLIQTGKVRNFPLILMGTDYWQPLLDFMCHDM
                     VREKTILPADCERILATDDPDEAMDRIMSAATGEFGLVWEPVPRWYFGESAMQKPSPP
                     SGAEPARKV"
     gene            1483..2160
                     /locus_tag="VEL74_17645"
     CDS             1483..2160
                     /locus_tag="VEL74_17645"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HYG64406.1"
                     /translation="MSQPAVAALAPEPFDFELVFDDGEPLESPWHRSQITFLEELLLQ
                     HLEEQRWTDVYVGANEFVYYSVEQARQVAERVGRNRPSEEVRGPDVFWVSGVDPTVER
                     EGWVSWREGGRLPDVIFELFSKSNTLAHRKQKKELYSRVFRTAEYFVYGPRSRRPEGF
                     RLIGGDYRAIEPDARGCLWSEQLHLFIGAWQGVFRRRSAHWIRFFRPDGSMMLTSREQ
                     LEQMHRL"
     gene            complement(2175..>3257)
                     /locus_tag="VEL74_17650"
     CDS             complement(2175..>3257)
                     /locus_tag="VEL74_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010611802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HYG64407.1"
                     /translation="GKFRVLTDENGPMDASEVFGSRKADHRILNATLALSRTEPERRV
                     VLVSKDINLRLKARALNLPSEDYETVKIKDVDHLYTGKQELEIEDPALIDRLYREESL
                     PVEEVLAAAPRANQYFILRSETKSALAYFNPRAGRLERVVKPLAYGISPRNAEQTFAL
                     HAVMNLDVPLVSLSGAAGTGKTLLALAGAMERRRDYRQIYLARPIVPLSNKDIGYLPG
                     DIQSKINPFMQPLWDNLGIIKNQYAEKDREYRKVDELVETEKLHIVPLAYIRGRSLAN
                     VIFIVDEAQNLTPHEVKTIITRAGDRTKVVLTGDIFQIDTPYLDTQSNGLSYLIDRIK
                     GNPLYAHVNLEKGERSELANLASSLL"
BASE COUNT          480 a         1085 c         1140 g          552 t
ORIGIN      
        1 ccggagatct cgtcgcgcag cgtccagttg ccgacgaagg ccgcggtccg gtagccgcgt
       61 ttgccgagcg tcgccgcggc cgatggcaaa ccccgccgca tgcgcaggcc gttgcgggtg
      121 gcgccgttct cgtgcgggta ggtggaggtg aacatggagc agagcgccgg gttggtgagc
      181 ggctccaccg tccgcgcctg ggtgaagcgg gccccggagg cgaggagctt atcgatcgcc
      241 ggcgacgtcg ggcggcggta gccgtaggag gacagacggt cggcgcgcag cgtgtcgacg
      301 gtgacgatga gcacgttggg gaagcgctgg gatgccgccc gcggctgggc gccggggatg
      361 gcggggagga ggagaccggc gagcccgaga agggcggcgg cgctccagag gtggatcgcg
      421 cgtcgtctca cggtgagacc ctctccgatt tggtaagctg cggtgtatga aggaggtgcg
      481 gcccgaaacc cagcccggac cggcgccggc ggtcgaggcc gaggcggcgg cccggagcgg
      541 cgagccgccg cgttggggca aggggaccga gagcccggac gacgggcgct tcctcgccgg
      601 cccgcagtcg cgggccgccg agctggtccg gctgtggcgg atctccgccg atttcatccg
      661 cggcttccgc gccctgcact tcgtcgggcc ctgcgtcacc gtcttcggct cggcccgctt
      721 ccccgaggac caccgctact acgccctggc acggcagatg ggccggcggc tgggccgcgc
      781 cgggttcacc accatgaccg gcggcgggcc gggggtcatg gaggcggcca accgcggtgc
      841 ccgcgacgtc ggcggccggt cggtgggctg caacatcgtc ctgccccagg agcagagccc
      901 caacccgtac ctcgacctct gggtggagtt ccgttacttc ttcgtgcgca aggtgatgct
      961 ggccaagtac tcgtacgcct tcgtggccat gcccgggggc ttcggcacca tggacgagct
     1021 cttcgaatgc gcgacgctga tccagaccgg caaggtccgg aacttcccgc tgatcctgat
     1081 ggggaccgac tactggcagc ctctgctcga tttcatgtgc catgacatgg tcagggagaa
     1141 gacgatcctg cccgccgatt gtgagcgcat cctcgccacc gacgaccccg acgaggcgat
     1201 ggaccgcatc atgagcgccg cgaccggcga attcgggctc gtctgggagc ccgtgccgcg
     1261 ctggtacttc ggcgaatccg ccatgcaaaa gccgagccca ccaagcggcg cggagcctgc
     1321 ccggaaagtc tagcaaaccc gcgcggccgg ctcgctgccc gcgcgaccgc cgtgctatcc
     1381 tctgtcgcgt aggaagacga gcggaaggcc tgcccgcccc tcccctgtac cggtgatccg
     1441 gtctatgatc tcccaaggaa cgacaccgaa agggactacg ccatgagcca gccagccgtc
     1501 gcggcgctcg cgcccgagcc cttcgatttc gagctcgtct tcgacgacgg agagcccttg
     1561 gagagcccct ggcaccgctc gcagatcact ttcctggaag agttgctgct ccagcacctg
     1621 gaggagcaac ggtggaccga cgtctatgtc ggcgccaacg agttcgtgta ctactcggtg
     1681 gagcaggccc ggcaggtcgc cgagcgggtg ggccggaaca ggccctccga ggaggtgcgg
     1741 ggccccgacg tcttctgggt gagcggcgtc gacccgaccg tcgagcgcga aggctgggtg
     1801 tcctggcggg agggtggccg gttgcccgac gtcatcttcg agctcttctc caagtccaac
     1861 acgctggccc accggaagca gaagaaggag ctctactccc gggtcttccg taccgccgag
     1921 tacttcgtct atggccccag gagccgccgg cccgagggct tccgcctgat cgggggcgat
     1981 taccgcgcca tcgagcctga tgcccggggg tgtctctgga gtgagcaact ccacctgttc
     2041 atcggcgctt ggcagggggt cttcaggcgg cggagcgccc actggatccg gttcttccgt
     2101 cccgacggct ccatgatgct cacctctcgc gagcaactcg agcagatgca ccgactgtaa
     2161 cccccactcc cccctcagag cagcgagctc gccaggttgg ccagctcgga gcgctcgccc
     2221 ttctccaggt tgacgtgggc gtagaggggg ttgcccttga tccggtcgat caggtaggag
     2281 aggccgttgc tctgggtgtc caggtagggg gtgtcgatct ggaagatgtc gccggtgagc
     2341 accaccttgg tgcggtcgcc ggcgcgggtg atgatggtct tgacctcgtg cggcgtcagg
     2401 ttctgcgcct cgtcgacgat gaagatgacg ttcgccaggc tgcggccgcg gatgtaggcc
     2461 agtggcacga tgtggagctt ctcggtctcc acgagctcgt ccaccttcct gtactcccgg
     2521 tccttctcgg cgtactggtt cttgatgatc cccaggttgt cccagagcgg ctgcatgaag
     2581 gggttgatct tcgactggat gtcccccgga aggtagccga tgtccttgtt cgacaacggc
     2641 acaatcggcc gcgcgaggta gatctgccgg tagtcgcgcc gccgctccat ggcgccggcc
     2701 agcgccagca gggtcttgcc cgtgcccgcc gcgcccgaca ggctcaccag cggaacgtcc
     2761 aggttcatca ccgcgtgcag cgcgaaggtc tgctcggcgt tgcgcggcga gatgccgtag
     2821 gccagcggct tgaccacccg ctcgagccgc cccgcccgcg ggttgaagta ggccagcgcc
     2881 gacttcgtct cgctgcgcag gatgaagtac tggttggccc gcggcgccgc ggcgaggacc
     2941 tcctctaccg gcagcgactc ctcccggtag agccggtcga tgagcgccgg gtcctcgatc
     3001 tcgagctcct gcttgcccgt gtagagatgg tcgacgtcct tgatcttgac cgtctcgtag
     3061 tcctcggacg gcaggttcag cgcccgcgcc ttcaaacgca ggttgatgtc cttgctcacc
     3121 aggaccacgc ggcgctccgg ttcggtccgg ctgagcgcca gggtggcgtt gaggatccgg
     3181 tggtcggcct tgcgcgagcc gaagacctcg ctcgcgtcca tcgggccgtt ctcgtcggtc
     3241 agcactcgga acttgcc
//
LOCUS       DASPJI010000630        10343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_267016, whole genome shotgun sequence.
ACCESSION   DASPJI010000630 DASPJI010000000
VERSION     DASPJI010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10343)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10343
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267016"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3326)
                     /locus_tag="VEL74_17655"
     CDS             complement(<1..3326)
                     /locus_tag="VEL74_17655"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HYG64408.1"
                     /translation="MSCRSHHPSRRLLPRGKAAAALGLAALTVLLLPGAPPASSNDVD
                     LLRFNTAKPYVFFILDTSASMTLSPQGQWVHANGDDPRSKLYQAKKVVYEVMSAVDDV
                     HFGFAAFNQDKSAVRAKHWLYYSTTALPGGWPINYPRPDADGPVQIQADGTAVSDVEG
                     DLMTFGGHVDATGIAGTCTAPLLLTSDLEKINRYAKLGASGNGPTKIWLKGGTGNKTY
                     RLTVTRPGNKPDTSLNQQLGRDGMNVRFQLEEVRASNGCTGPDTANFQATHNLNLDLK
                     LWTDFLMFDEPNGVTAPGTSKAGGVDPVAGYWDYKDILDTASCGSGHPFSGKGWEGNY
                     DGASGSVPPASVTPSLKPTDDPYCLVAGDPSTCYNVKRPTTYDPFGRPLDRGDVLPLD
                     WRTENKTEFLSRLAPSQSDGAPDFRIASYFKDFPEASTGVLALKESGESPLMGTGPSP
                     LGKSVIDFRCWYLGDGNKCNDSAYAPGWEAVAQRYDKEWGCRRPYLIVISDGGDSCAG
                     ENPCADTANLNSKGGIRTWVMAYGADCSKAGNPLKCMAQNGKGELVCPQTANDLKAEL
                     QRILGLIREEARSFASAAVPSVQAIVEDKIFLTNFTPLNGRSTWDGHVHSFLKPLPLR
                     PTDNRPDLAHENHLWDAGEVLKTQVKASDPVGPADDQRRVYYPTRVSGVPGRLLEPVP
                     LDDSTATGKALRRDLWDGFGIGYTPGDFVTEAAAEIKVRSVLQTTYSLKTHTTTVTDP
                     VTGKPTTKTLQYVLGDIFHSNPLVIGSPSNTRYFADDASGYRDFFRQQEMRRKLLLVG
                     GNDGMMHAFEAGLPRVLTGTDPTTGQSRKYVQFDNGSGKEVFAFMPREVMPAVRKMAE
                     SNSHQWGVDGTVSVGDVYIDPLRKSTPERSWRTVVVGGLREGGSSYYALDITAPDKVS
                     LENLTDGDTYVPTADGTYRPSCAGDGGAYNAARCGPVPFPYVLWEFTDAVKNVGTGAL
                     VRLDEDLNGHADLGYTWSVPNIGRIRVREGGDVVDKYVAIVGGGFDPSDKLNPKRGTW
                     LYMIDVETGQAIYKRQLVGAVPSEPAAVDTNQDGYLDRIYVGTTLGKVYRVDLNQDAA
                     GEYPALANTSVRGLDGATYS"
     gene            complement(3339..3938)
                     /locus_tag="VEL74_17660"
     CDS             complement(3339..3938)
                     /locus_tag="VEL74_17660"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HYG64409.1"
                     /translation="MREPIEANPRRVKRRAEAGSAYIAVLLVLVILTILGLALSFVTQ
                     TEMQTGRNERTVNRVFYAADAGVETAIARALVASDHVGRSFFFTDSGQSLAPGTLELG
                     TEVAISDFFPIQEGPCNFCEINNGGGYQGRDYYNVTHAVTIRATRFATVDAGTARKAV
                     AQKTVSTMVDIQPWKTSNAILSKTIENEASLKEISIGMN"
     gene            complement(3954..5225)
                     /locus_tag="VEL74_17665"
     CDS             complement(3954..5225)
                     /locus_tag="VEL74_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYG64410.1"
                     /translation="MKRAPARPSRPAAGFSLVELLVTLLVTSVLLLAVLATFDFNSRV
                     SRVQTSVADMQQSLRVSQQEMVRWIRMAGRGGLRTAEPARGLFPPDGLALEVANNVAA
                     DTEIVPGNPLTRVVEGTDVVTVRGVFTSPLYQLNYTEPATLTLAGGGPSTPPTTGTIV
                     VNDTAVRGVPQNLAALKEAIDRNVPEAILLVSPFDDLFAVVELVPAASSYDAGTVRLG
                     FRTTGTRGALYGQLSAGGSYPIGLQSVNYLGILEEYRFYVREAHLTPGDETTQLMPKL
                     ARARMYPGTDEPWVDTDNATVDVADNILDLQVALGFDSANSGGATGTVMEAAGGTTDD
                     WMLNRPGAAGITAELNEPAFRTNPTPPLYYLRLSTLARTERRDPKYLAPVIEAIEDHE
                     YPATETGINSPEERAFRRRVLQTVIDLRNLG"
     gene            complement(5237..5812)
                     /locus_tag="VEL74_17670"
     CDS             complement(5237..5812)
                     /locus_tag="VEL74_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64411.1"
                     /translation="MQTERTIQPRAAANIRSGGFSVIEVLIASAIFLVIAVGLLPLFA
                     QSITSNLAGRETTDTTNHGRSRLEEIDQLSFFNPNLTVPAGETEGTVQEYWSQKDKIW
                     KAGAPPLADPALWMRTTRVRQFSVSDLNDDGTFDSPLDGDAAPGQVHLKEIQVAVRSA
                     REGTQLADGTWSTRSGLGGIWESRMRTLKAK"
     gene            complement(6010..7818)
                     /locus_tag="VEL74_17675"
     CDS             complement(6010..7818)
                     /locus_tag="VEL74_17675"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG64412.1"
                     /translation="MTGQGSRSWRRLGLRREVLILLPVTLLLLVILSTFTLFSYRNAL
                     SSLAQERRDDAAHLARTVADQLAVRGAGTAGGERVPSPGELRALSPGARAAALVDAAG
                     QPLAVVGELPPADLLPPAALEAAEPVGIGPDESLPGVVAGFAPLGGADRRRYVRVDLP
                     AEALARQLRAIRVLTWVVLAVDVNLVLLVLLFLRHLLAPYETLLARAQAIGGPGEKGQ
                     DEVAFLLATFDRAVSALAARDARRPEDDIAALERTLSASLQSGLLLLDREGRVLALNA
                     VGAELLGTTPPAPGARLEEVLAGQPELLDLLAAAVAEGNAPRRQECTVRAGGADRTSR
                     TVGLTVHPLRRDDGGVRGHLVLFADLTEVQRRAEEARLAESLARLGEMAGGVAHELRN
                     SLATLRGYLTLIERRPDEESIADYLSEIRHEADHLERVLEDFLAFARPGTARVQEFPL
                     TPLLRRAAADPSLAGVQVTADTAAADGVTLRGDPQLLERAVRNLLHNAVQAERESGRS
                     GPVEVWAAPSPEGVEVAIQDRGPGLPAEIRERLFHPFATGRRGGVGLGLALAHRIVVL
                     HGGRIRLEDRPGGGTQALLTFPPDTLVTTSNTTALS"
     gene            complement(7815..8588)
                     /locus_tag="VEL74_17680"
     CDS             complement(7815..8588)
                     /locus_tag="VEL74_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HYG64413.1"
                     /translation="MQLQILVALYAAVLGLIVGSYLNVVIYRLPLGISTVTPRSRCPA
                     CGAAIRPWDNVPVVSWLLLGGRCRACRAKISWRYPAVEAFTSLLFVASFLRFGWSAAT
                     PAAALFCCLMVALAMIDFDHMILPDRITLPGIAVGLALQPWLPWGGLRQAVIGAVLGA
                     AILLAVWGGWYLIRKEEGMGLGDVKMLALMGAFLGWKGVLVALFFGTLTGAVAGVALM
                     RWGTLDMRSKLPFGTFLALGGLIALFFGQPLADYYAGLL"
     gene            complement(8667..9293)
                     /locus_tag="VEL74_17685"
     CDS             complement(8667..9293)
                     /locus_tag="VEL74_17685"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HYG64414.1"
                     /translation="MARGTATEEMGRDLLDVREDDYEPVALDPEHWQMAGHGGFHFLE
                     GEAESDGGPGILWYTREELGDFVLKIDWRARSATDNSGVFLRIPALGTGNPDKDWKPA
                     VDLGYEVQIDDRGRSPETGGEGDSFRTTGAVYGLAAAGQLLSRPVGQWNTFEIEARGN
                     SVTVFLNGERASHFSGDSGRKTRGYLGLQNHSPGSRVAFRNLRIKRLQ"
     gene            complement(9297..9806)
                     /locus_tag="VEL74_17690"
     CDS             complement(9297..9806)
                     /locus_tag="VEL74_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HYG64415.1"
                     /translation="MRAKARLFGHPIHQMLIVFPLGLLGTSLFFDIAHLVSGNSQWAL
                     IAYWMIVAGILSALVAAVFGAIDWLAIPSGTRAKRIGALHGGGNLVVVLLFVVSWLMR
                     RDDPLAPEGLAIALSGLGVLLSLVTGWLGGELVDRLGVGVDEGANLDAPSSLSGRPAA
                     GAGVPPGRR"
BASE COUNT         1496 a         3491 c         3821 g         1535 t
ORIGIN      
        1 tggctgtacg tcgccccgtc gaggccgcgg acgctggtgt tggccagcgc cgggtactcg
       61 ccggcggcgt cctggttgag gtcgacgcgg tacaccttgc ccagggtcgt gccgacgtag
      121 atccggtcga gatagccgtc ctggttggtg tcgaccgccg ccggctcgct gggcacggcg
      181 ccgacgagct gccgcttgta gatggcttgc ccggtctcga cgtcgatcat gtagagccag
      241 gtgccgcgct tgggattgag cttgtcgctg ggatcgaagc cgccgccgac gatggcgacg
      301 tacttgtcca ccacatcgcc cccctcgcgg acgcggatcc gcccgatgtt ggggaccgac
      361 caggtgtagc ccaggtcggc gtggccgttg aggtcctcgt ccagccgcac cagagcgccg
      421 gtgccgacgt tcttgacggc atcggtgaac tcccagagca cgtacgggaa cggcaccggg
      481 ccgcagcgcg ccgcgttgta ggcgccgcca tcgccggcgc aggacggccg gtaggtgccg
      541 tcggcggtcg gaacgtaggt atcgccgtcg gtcaggttct ccagggagac cttgtccggc
      601 gcggtgatgt ccagggcgta gtaggacgac ccaccctcgc gcaggccgcc gaccaccacc
      661 gtgcgccagc tccgctccgg cgtcgacttg cgcagcgggt cgatgtagac gtcgcccacc
      721 gacacggtgc cgtccactcc ccactgatgg ctgttgctct cggccatctt ccgcaccgcg
      781 ggcatcacct cccgtggcat gaaggcgaag acctccttgc cgctgccgtt gtcgaactgg
      841 acgtacttgc gggactgccc ggtcgtcggg tcggtgccgg tgaggacgcg cggcaggccg
      901 gcctcgaagg cgtgcatcat gccgtcgttg cccccgacca gcaggagctt gcgccgcatc
      961 tcctgctgcc tgaagaagtc gcggtagccg ctggcgtcgt cggcgaagta gcgggtgttg
     1021 ctcgggctgc cgatgaccag cgggttggag tggaagatgt cgcccagcac gtactgcagc
     1081 gtcttggtgg tgggcttgcc ggtcaccggg tcggtgaccg tggtggtgtg ggtcttgagg
     1141 gaataggtcg tctgcagcac cgaccggacc ttgatctcgg ccgcggcctc ggtcacgaaa
     1201 tcccccggcg tatagccgat cccgaagccg tcccagaggt cgcgccgcaa cgccttgccg
     1261 gtcgccgtgg agtcgtcgag ggggaccggc tcgaggagcc gtccggggac gccgctcacc
     1321 cgggtcgggt agtagacccg ccgctggtcg tccgccggcc ccacggggtc cgaggccttg
     1381 acctgcgtct tgagcacttc gccggcgtcc cacaggtggt tctcgtgcgc gaggtcgggc
     1441 cggttgtcgg tcggacgcaa cggcagcggc ttcaggaacg aatgcacgtg gccgtcccag
     1501 gtcgacctgc cgttgagcgg cgtgaagttg gtgaggaaga tcttgtcctc gacgatcgcc
     1561 tggaccgacg gcacggcggc ggaggcgaac gagcgggcct cctcgcggat caggccgagg
     1621 atccgctgca gctcggcctt gaggtcgttc gccgtctgcg ggcagaccag ctcgcccttg
     1681 ccgttctgcg ccatgcactt caacgggttg ccggccttcg agcagtcggc gccgtaggcc
     1741 atcacccagg tgcggatgcc ccccttggaa ttgaggttgg cggtgtcggc gcaggggttc
     1801 tcacccgcgc agctgtcgcc gccgtcgctg atgacgatca ggtagggacg ccggcagccc
     1861 cactccttgt cgtagcgctg ggccaccgcc tcccagccgg gagcgtaggc ggagtcgttg
     1921 cacttgttgc cgtcccccag gtaccagcag cggaaatcga tcaccgactt gccgagcggg
     1981 ctcgggccgg tgcccatcaa gggggattcg ccgctctcct tgagcgccag gacgcccgtg
     2041 gaagcttccg ggaagtcctt gaaataggag gcgatccgga aatcgggggc gccgtccgac
     2101 tggctgggcg ccaggcggct caggaactcg gtcttgttct ccgtgcgcca gtcgagcggc
     2161 aggacgtcgc cgcggtccag tggccgtccg aagggatcgt acgtcgtcgg ccgcttgacg
     2221 ttgtagcagg tcgacgggtc tccggccacc aggcagtacg gatcgtccgt cggcttgagg
     2281 ctcggcgtca cgctggccgg cgggacgctc cccgaggcgc cgtcgtagtt gccctcccag
     2341 cccttgcccg agaacgggtg gcccgagccg caggaggcgg tgtccaggat gtccttgtag
     2401 tcccaatagc ccgccaccgg gtcgacgcct ccggccttgc tggtgccggg agcggtgacg
     2461 ccgttgggct cgtcgaacat caggaagtcg gtccacagct tgaggtcgag gttcaagttg
     2521 tgagtggcct ggaagtttgc cgtgtcgggg ccggtgcagc cgttgctggc cctgacctcc
     2581 tccagctgga acctgacgtt catgccgtcc cggcccagtt gctggttgag gctggtgtcc
     2641 ggcttgttgc ccggccgggt gacggtcagg cgataggtct tgttgccggt gccccccttg
     2701 agccagatct tggtcgggcc gttgccgctc gcgcccagct tggcgtagcg gttgatcttc
     2761 tcgaggtcgc tggtgaggag gagcggcgcc gtgcaggtgc cggcgatgcc cgtggcgtcc
     2821 acgtggccgc cgaaggtcat caggtccccc tcgacatcgc tcaccgcggt gccgtcggcc
     2881 tggatctgga ccggcccgtc ggcgtcggga cgcgggtaat tgatcggcca gccgccgggc
     2941 agggccgtgg tgctgtaata cagccagtgc ttcgcccgca ccgccgactt gtcctggttg
     3001 aaggcggcga agccgaagtg gacgtcgtcg acggccgaca tgacttcgta gacgaccttc
     3061 ttcgcctggt agagcttgga gcgcgggtcg tcgccgttgg cgtgcaccca ctgcccctgg
     3121 ggcgacaggg tcatcgacgc cgaggtgtcg aggataaaga agacataagg cttcgcggtg
     3181 ttgaaccgca gcaggtcgac gtcgttcgac gaggccggcg gtgcaccggg cagcaacagc
     3241 acggtcaggg cggcaaggcc gagggcggct gcggccttgc cacgcggcag caaccgccgc
     3301 gacgggtgat gggaacggca ggacatggaa tcctcctcct agttcatccc gatcgaaatc
     3361 tctttgagac tggcctcgtt ttcgatcgtc ttactcaaaa tggcgttcga agtcttccag
     3421 ggctggatgt ccaccatggt ggagaccgtc ttctgggcca cggccttgcg cgcggtaccc
     3481 gcgtccacgg tggcgaacct ggtcgcccgg atcgtcacgg catgggtgac gttgtagtag
     3541 tcccgcccct ggtagccgcc gccgttgttg atctcgcaga agttgcaggg accttcctgg
     3601 atcgggaaga agtcggagat ggcgacctcg gttcccagct ccagggtgcc cggggccagc
     3661 gactgcccgg agtcggtgaa gaagaagctc cggcccacgt gatcgctggc caccagggcg
     3721 cgggcgatgg ccgtctcgac gcccgcgtcg gcggcgtaga acacccggtt gacggtgcgc
     3781 tcgttgcgcc ccgtctgcat ctccgtctgg gtgacgaagg acagggcgag gccgaggatg
     3841 gtgaggatca ccagcaccag cagcacggcg atgtaggcgc tgccggcctc ggcccgccgc
     3901 ttcactcgcc tcgggttcgc ttcgatgggc tcgcgcatga tgtctctcct cggtcagccc
     3961 aggtttcgca ggtcgatgac ggtctgcagc acccgccggc ggaacgcccg ctcctcgggg
     4021 ctgttgatcc cggtctccgt agctgggtac tcgtggtcct cgatcgcctc gatgaccggt
     4081 gccaggtact tggggtcgcg ccgctccgtg cgggccaggg tgctgagacg caggtaatag
     4141 aggggcggcg tcgggttcgt ccggaaggcc ggctcgttga gctccgccgt gatgcccgcg
     4201 gcaccgggtc ggttgagcat ccagtcgtcg gtggtgccgc cggcggcctc catcaccgtg
     4261 ccggtggcgc cgccggagtt ggcggagtcg aagcccaggg ccacctgcag gtcgaggatg
     4321 ttgtccgcca cgtcgacggt ggcgttgtcc gtgtccaccc agggctcgtc ggtgccgggg
     4381 tacatccgcg cgcgggcgag cttcggcatg agctgcgtcg tctcgtcgcc gggggtcaga
     4441 tgggcctccc ggacgtagaa ccggtactcc tccagaatgc ccaggtagtt gacgctctgc
     4501 agcccgatcg gataggagcc gccggcggag agctggccgt acagggcgcc gcgcgtgccg
     4561 gtggtgcgga agccgagccg cacggtcccg gcgtcgtagg agctcgccgc gggcaccagc
     4621 tccaccaccg cgaacaggtc gtcgaagggg ctcaccagca ggatcgcctc ggggacgttg
     4681 cggtcgatgg cctccttcag ggcggccagg ttctgcggca cgccgcgcac cgccgtgtcg
     4741 ttgaccacga tggtccccgt cgtcgggggg gtcgagggcc cgccgcccgc cagggtcagg
     4801 gtggccggct ccgtgtagtt gagctggtag agcggcgagg tgaacacgcc gcgcacggtc
     4861 accacgtcgg tcccctccac cacccgggtc agcgggtttc ccggcacgat ctcggtatcc
     4921 gcggcgacgt tgttcgccac ctcgagcgcc aggccgtccg gcgggaagag gccgcgcgcc
     4981 ggctcggcgg tgcgcagccc gccgcggccg gccatgcgga tccagcgcac catctcctgc
     5041 tgtgacacgc gcagcgactg ctgcatgtcg gcgacgctgg tctgcacccg cgacacccgg
     5101 ctgttgaagt cgaaggtggc gagcaccgcc agcagcagca ccgaggtgac cagcagggtg
     5161 accagcagct cgaccagcga gaagccggcc gcggggcgag agggacgagc aggggcgcgc
     5221 ttcatgatcg gctccctcac ttggccttga gcgtccgcat ccggctctcc cagatgcctc
     5281 cgagcccgga gcgggtcgac caggtcccgt ccgccagctg ggtaccctcg cgggcgctgc
     5341 gcacggcgac ctggatctcc ttgaggtgga cctggcccgg ggccgcgtcg ccatcgagcg
     5401 ggctgtcgaa ggtgccgtcg tcgttgagat cggacaccga gaactgccgc acccgggtgg
     5461 tacgcatcca gagggccgga tcggccagcg gcggcgcgcc ggccttccag atcttgtcct
     5521 tctgcgacca gtactcctgg acggtcccct cggtctcgcc cgcggggacg gtcaggttcg
     5581 ggttgaagaa ggagagctga tcgatctcct ccaggcggga ccggccgtgg ttggtcgtgt
     5641 ccgtcgtctc ccggcccgcc aggttgctgg tgatggactg cgcgaagagg ggcagcaggc
     5701 ccaccgcgat gaccaggaag atggccgagg cgatcagcac ctcgatcacg gagaagccac
     5761 cgctgcggat attggcggcg gctctcggct ggatcgtccg ttcggtctgc acgactcgca
     5821 acctccgtaa ggccgcggct cggccgcggc gcgaagactt cgactgctgg gtgctgctcg
     5881 ggtacgtcgg ggtacggcga gacccctaga gagcaagggg ggtgccacgg ctccgccggc
     5941 ggggcgggag gggctcaacc gactgaaaca gcaggacttc ggagcggcag gagcggcgag
     6001 gggcgagatt caggagaggg cggtcgtgtt actcgtggta acaagagtgt cggggggaaa
     6061 ggtgagcagg gcctgggtcc cgccccccgg ccggtcctcc agacggatgc gcccgccgtg
     6121 caggacgacg atgcggtggg ccagcgccag ccccaggccg acgccgccgc gccggccggt
     6181 ggcgaagggg tggaacaggc gctcccggat ctcggccggc aggccggggc cgcggtcctg
     6241 gatcgccacc tccaccccct cgggcgaggg ggcggcccac acctccaccg gaccggaccg
     6301 gccgctctcg cgctccgcct ggacggcgtt gtgcagcagg ttgcgcaccg cgcgctcgag
     6361 gagctggggg tcgccgcgca gggtgacgcc gtcggcggcg gcggtgtcgg cggtcacctg
     6421 cacaccggcc agggaggggt cggcggcggc gcgccgcagc agcggggtga gggggaactc
     6481 ctggacgcgg gcggtgccgg ggcgggcgaa ggcgaggaag tcctccagca cccgctccag
     6541 gtggtcggcc tcgtggcgga tctccgaaag gtagtcggcg atcgactcct cgtcggggcg
     6601 gcgctcgatc agggtgaggt agccgcgcag ggtggcgagg ctgttgcgca gctcgtgggc
     6661 gacgccgccg gccatctcgc cgaggcgggc gaggctctcg gccaggcggg cctcctcggc
     6721 gcggcgctgc acctcggtca ggtcggcgaa gaggacgagg tggccgcgca cgccgccgtc
     6781 gtcgcggcgc agggggtgga cggtgagccc gacggtgcgg ctggtgcggt cggcgccgcc
     6841 ggcgcgcacg gtgcactcct ggcgccgcgg cgcgttgcct tcggcgacgg cggcggccag
     6901 caggtcgagg agctccggct ggccggccag cacctcctcc aggcgggcgc cgggggccgg
     6961 cggcgtggtg cccaggagct cggcgccgac ggcgttcaag gccaggaccc gcccctcgcg
     7021 gtcgagcagc aggaggccgc tctgcaggct ggccgagagc gtccgctcca gggcggcgat
     7081 gtcgtcctcg ggccggcggg cgtcgcgggc ggcgagcgcc gagaccgcgc ggtcgaaggt
     7141 ggcgaggagg aacgccacct cgtcctgccc cttctctccg gggccgccga tcgcctgcgc
     7201 ccgggcgagc agcgtctcgt acggcgccag gaggtgacgc aggaagagga ggacgaggag
     7261 gacgaggttc acgtccacgg cgaggacgac ccaggtgagc acgcggatgg cgcggagctg
     7321 gcgggccagc gcctcggccg ggaggtccac ccgcacgtag cgccggcgat ccgcccctcc
     7381 caggggggcg aagccggcga cgacgccggg cagggactcg tccggcccga tgccgacagg
     7441 ctccgcggct tccagggcgg caggtgggag gaggtccgcc ggcggcagct cgcccaccac
     7501 ggccaggggc tgcccggcgg cgtcgaccag ggcggcggcg cgggcgccgg gggagagagc
     7561 ccgcagctcg ccggggctgg gcacccgctc gccccccgcg gtgccggcgc cgcgaaccgc
     7621 gagctggtcg gccacggtgc gggcgaggtg ggcggcgtcg tcccggcgct cctgggcgag
     7681 cgaggagagg gcgttgcggt aggagaacag ggtgaaggtg gagaggatga cgaggagcag
     7741 cagggtcacc ggcagcagga tcagcacctc gcgccgcagc ccgaggcgcc gccagctccg
     7801 gctcccctgc ccagtcacag cagtcccgcg tagtagtcgg ccagcggctg gccgaagaac
     7861 agggcgatca gcccgccgag ggcgaggaag gtgccgaagg ggagcttgga gcgcatgtcc
     7921 agcgtccccc agcgcatcag ggcgactcct gccacggcgc cggtcagggt gccgaagaag
     7981 agggcgacga gcaccccctt ccagccgagg aaggcgccca tcagggcgag catcttgacg
     8041 tcgcccaggc ccatcccctc ctccttccgg atcaggtacc agccgcccca gaccgcgagc
     8101 aggatggcgg cgcccagcac ggcgccgatc accgcctggc gcaggccgcc ccacggcagc
     8161 cagggctgga gggcgagccc gacggcgatc cccggcagcg tgatccggtc cggcaggatc
     8221 atgtggtcga agtcgatcat cgccagggcg accatcaggc agcagaacag ggcggcggcg
     8281 ggcgtggcgg cgctccagcc gaagcgcagg aagctggcga cgaagaggag ggaggtgaag
     8341 gcctcgaccg ccgggtagcg ccacgagatc ttcgcccggc aggcgcggca gcggccgccc
     8401 aggagcagcc acgagaccac cggcacgttg tcccacgggc ggatcgccgc gccgcacgcc
     8461 gggcagcgcg agcgcggggt gaccgtggag atccccagcg gcagccggta gatcaccaca
     8521 ttcaggtagc tgcccacgat caggccgagg accgcggcat acagggcgac cagaatctgc
     8581 agttgcacgg cggggattct acccgctgat cgctccgaac accgctccga acatcgctcc
     8641 ggaaaccgcg gccccggcca agccgtctac tgcagccgct tgatccgcag gttgcggaag
     8701 gcgacccgcg agcccgggct gtggttctgc agccccaggt agccccgcgt cttgcggccc
     8761 gaatctccgg agaagtgact ggcccgctcg ccgttgagga acaccgtgac cgagtttccc
     8821 cgggcttcga tctcgaacgt gttccactgt cccaccggcc gggagaggag ctgccccgcc
     8881 gccgccagac catagaccgc cccggtggtg cggaacgagt ccccctcgcc gccggtctcg
     8941 gggctgcggc cgcggtcgtc gatctgcacc tcgtagccga ggtcgacggc gggcttccag
     9001 tccttgtccg ggttgccggt gccgagggcc gggatgcgca ggaagacccc cgagttgtcg
     9061 gtggcgctac gggcgcgcca gtcgatcttg aggacgaagt cgccgagctc ctcgcgggtg
     9121 taccagagga tccccgggcc gccgtcggac tcggcctccc cttccaggaa gtggaagccg
     9181 ccgtgcccgg ccatctgcca gtgctcgggg tccagggcca ccggctcgta gtcgtcctcg
     9241 cggacgtcca gcaggtcccg ccccatctcc tccgtcgcgg tgcctctcgc catcgcctac
     9301 ctccttccgg ggggcacacc cgcccccgcc gccgggcgtc cggagaggga gctcggggca
     9361 tcgaggttcg cgccctcgtc gactccgact cccagacggt cgaccagctc gccacccagc
     9421 cagccggtca ccagcgacag tagcactccc agcccggaca gcgcgatcgc cagcccctcc
     9481 ggcgccaggg ggtcgtcgcg ccgcatcagc cagctcacca cgaacagcag aaccaccacc
     9541 aggttgcccc cgccgtgcag cgcgccgatc cgcttcgccc gggtgcccga tgggatcgcc
     9601 agccagtcga tcgccccgaa gaccgccgcc accagggcgg agaggatgcc ggcgacgatc
     9661 atccagtagg cgatcagggc ccactgcgag ttgccgctca ccaggtgggc gatgtcgaag
     9721 aacagcgagg tgcccagcag gcccagcggg aagacgatca gcatctgatg aatgggatgg
     9781 ccgaacagac gtgccttggc tctcatccac acctcccgat cgatcggggc tccggcgaca
     9841 cgccggccct agtctccgga aggtgcaacg gccatgccgt gaagggggga ggtcgagact
     9901 cgcgcggggc tggtgatgcc tccccccctg ggcggttggg cggaagggag cgggggaggt
     9961 ccacgggggt gaagggcgaa cggctcgacc cacggggcag atggcttgac ccgcgggtca
    10021 aacggcgtgg atcgcggggt cgtcgtcgta cctcgagaaa tggccgccgt acccggggaa
    10081 aggatcgcca tacccggggt aggaccttcg caccccggca gatcgcctcc gtaccccggg
    10141 gatgatcgtc atcccccgag ggatgaacgc cgtgcccgcg ggtatggctc tcgtccacga
    10201 gggagggtca ccgtaccccg ggtagggcct ccgtacctcc ggggaggagc gtcgcctcgc
    10261 gggggatggc cgtcgggaaa tgaagaaaaa ctccgtctcg gccttccccc tcccggtatc
    10321 gcgagcccaa gctgacgagg cgc
//
LOCUS       DASPJI010000631         5522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_439326, whole genome shotgun sequence.
ACCESSION   DASPJI010000631 DASPJI010000000
VERSION     DASPJI010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439326"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VEL74_17695"
     CDS             <1..1098
                     /locus_tag="VEL74_17695"
                     /inference="COORDINATES: protein motif:HMM:NF020071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HYG64416.1"
                     /translation="RAADLPGGGHLNAGAARFVLKARDQLADTLRRELGKAPDAADRD
                     DAVLRALFGAYSDRLARRREPRSPRGVMVGGRGVHLADESAVLEAGLFLCIDITDPPG
                     TGSRAESLVRIASAVEPDWLPADRLRTGLEVDFDEARERVIAWKRTRFDDLVLSEAEV
                     PAEGEAAERVLAQAAAWRLDRALPLDDPEVASFLARARCLREWMPELGLPAFGDDDLR
                     ALLPALAAGRRSFADLRRSPLLDALRGTLTWPQLEALDREAPERLEVPSGSRVKLVYE
                     PGRPPVLAARIQEMFGLADTPRLAAGRVPVLLHLLAPSGRPQQVTHDLRSFWENTYPQ
                     VKKELQGRYPKHAWPQDPWTAQPQRRPGRRG"
     gene            1207..1374
                     /locus_tag="VEL74_17700"
     CDS             1207..1374
                     /locus_tag="VEL74_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64417.1"
                     /translation="MKKTVKKLVLTKETVRGLEEKGLDKAAGASAYICVVDTNYNSCW
                     RYCQREPNIYG"
     gene            1761..1922
                     /locus_tag="VEL74_17705"
     CDS             1761..1922
                     /locus_tag="VEL74_17705"
                     /inference="COORDINATES: protein motif:HMM:NF038153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I lanthipeptide"
                     /protein_id="HYG64418.1"
                     /translation="MKKQTKKLVLSKETVRDLEASALGKAAGGATVLCTTDPFRFCPN
                     MPNKSYDVC"
     gene            2790..3134
                     /locus_tag="VEL74_17710"
     CDS             2790..3134
                     /locus_tag="VEL74_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64419.1"
                     /translation="MPDHDDRTPSRVDLSRPTESTRRHLYDLVVHVYFHPTPEEVEKA
                     KADPGNHWVPDYGPDAARLTVFYAYGRWFAVWEALEEKETGLPEWRRWSVVRIVEDRD
                     PSSATGVTFLEV"
     gene            3491..4516
                     /gene="ruvB"
                     /locus_tag="VEL74_17715"
     CDS             3491..4516
                     /gene="ruvB"
                     /locus_tag="VEL74_17715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004374647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HYG64420.1"
                     /translation="MHLEDGLLSGVPDPEDLGVEPSLRPQRLDEYIGQRKILDNLRVF
                     IRAARERRESLDHVLLFGPPGLGKTTLAHIIATEMQAPLRLTAGPVLERAGDLAAILT
                     NLEPGEVLFIDEIHRLGPTVEEILYPALEDFRLDLVIGQGPAARTVKIDLPRFTLVGA
                     TTRAGLISAPLRARFGIVHRLDFYRPEELKLIVERSSRLLSIGIDPAGALEIARRSRG
                     TPRVANRLLRRVRDFAQVERADRIELEIARKSLALLEVDEYGFEEVDRKILGTLIEHY
                     GGGPAGLKALAAAVGEDSGTLEDLYEPYLIQEGFLQRTPRGRVATRRAYEHLGYPPRP
                     GSMGTLF"
     gene            4634..5515
                     /locus_tag="VEL74_17720"
     CDS             4634..5515
                     /locus_tag="VEL74_17720"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HYG64421.1"
                     /translation="MRRAALIYNPRAGRQRQAEGLDGIVAALRSGGCFAEPVPTRGPG
                     DATTLARELAARGDVEAVFAFGGDGTVREVAAGLLGSGVPLGILPGGTVNLLAMALGL
                     PPDPVAAAAVLAGAPARPMDVGLAGASPFLMMVSAGLDASVLAAVDPRLKLHFGQGAV
                     LGHGLVEWWRYGYPPLQVVADGERLEATFAAVSNIPYYAGAFHLAPGARLDDRRLDLV
                     LFRGTGRVATVLFALDLLRAAHVRRRDVLIRPVEEVVFTGPAGAATQVDGDLCQETVP
                     LTVRLAPETIPVLMPAV"
BASE COUNT          808 a         2046 c         1843 g          825 t
ORIGIN      
        1 cgcgccgccg acctccccgg agggggccac ctgaacgcgg gagccgcccg cttcgtcctc
       61 aaggcccggg accagctcgc cgacaccctc cgccgggagc tggggaaggc tccggacgcc
      121 gccgaccggg acgacgccgt cctccgcgcc ctcttcggcg cctactcgga ccgcctcgcc
      181 cgccgccgcg agccgcggag cccgcgcggc gtcatggtgg gaggccgggg ggtccacctc
      241 gccgacgaga gcgccgtcct ggaagccggc ctcttccttt gtatagacat cacggacccg
      301 ccgggcacgg ggtcccgcgc cgagtcgctg gtgcgcatcg cctcggccgt cgagccggac
      361 tggctcccgg ccgaccgcct gcggaccggg ctggaggtgg acttcgacga ggcccgcgag
      421 cgggtgatcg cctggaagcg gacgcgcttc gacgacctgg tgctgtccga ggccgaggtg
      481 ccggccgagg gggaggcggc ggagcgggtg ctggcccaag cggcggcttg gcggctggac
      541 cgggcgctcc ccctggacga ccccgaggtc gcctccttcc tcgcccgggc gcgctgcctg
      601 cgcgagtgga tgcccgagct gggcctcccc gccttcggcg acgacgatct ccgcgcgctc
      661 ctccccgccc tcgccgccgg ccgccgctcc ttcgccgacc tccgccggtc gccgctcctg
      721 gacgccctgc gcggcaccct cacctggccg cagctcgaag cgctggaccg ggaggcgccg
      781 gagcggctgg aggtgccgag cggcagccgc gtcaagctcg tctacgagcc gggccgtccc
      841 ccggtcctgg cggcgcgcat ccaggagatg ttcggcctgg cggacacccc gcgcctcgcc
      901 gccggccgcg tccccgttct cctccatctc ctcgccccga gcggccggcc gcagcaggtg
      961 acccacgacc ttcgcagctt ctgggagaac acctatcccc aggtgaagaa agagctccag
     1021 ggccgctacc ccaagcacgc ctggccccag gacccctgga ccgcccagcc ccagaggcgg
     1081 ccgggacggc ggggataggc tttacggcgt ctccttacat agatcgaagt caagcctgtt
     1141 tctcaaggcc gatcggattg agaatcatgg ctcgccgaag ggcgtatcaa cgacaggagc
     1201 gaagaaatga agaagactgt caagaagctg gtgctgacga aggagacggt gcggggcctc
     1261 gaggagaagg ggctggacaa ggcggcaggc gcgagcgcct acatctgcgt cgtggacacc
     1321 aactacaact cctgctggcg ctactgccag cgcgagccca acatctacgg ttgaggcttc
     1381 ctgggccctc cggcctggag gctggagggc cccaccaacc acaggggatc gagatgtgcg
     1441 agcatcaggg ccatggcgac ggagcagccc tctcccttcg acactcccgc ggaccgaccc
     1501 gaggagccgc aggagccaca gaccgccacg gatgacgacg ccctgatcct ctggatgctc
     1561 agtctgactc ccacccagcg gctgagtcgc tcagggcttc gtcgacagcg tccaggcgct
     1621 gcggaatgtg ggtccgcttc ggcgaattga gcagggacgg tgagagtgcc ggctccggcc
     1681 gaagtcaagc cttcccttca agctctgtga ggtcgagaat gccttgcgcc aatcggcggt
     1741 caaccaggga aggagcgacg atgaaaaagc agacgaagaa gctggtgttg tcgaaggaga
     1801 cggtgaggga cttggaggcg agtgcgctcg gcaaggccgc aggcggggcc accgtgctgt
     1861 gcacgaccga ccccttcagg ttctgtccca acatgcccaa caagtcctac gacgtctgct
     1921 aggacaccgg ccctccggct ccggccggag ggctccctcc accacccgca ctgaataagg
     1981 acaccactct gcggcgtacc agttcagcgc ggctccggag tagtccgctg ccgcgccgta
     2041 ccacttcggg acggccccgt accacttcgc tgcggcgccg tagtgcttcc ccgccgcagc
     2101 gaagcactac gcggcgactc cgaagtactt ccccgcgcct cccgaacact cccattcgat
     2161 ttcgtagcac tgcggagcca cggcgaacta cttcggagcc gcgccgtact acttcgctgc
     2221 cgttccggag tactccgccg ccgttcccga acactccaat ccgactccgt accacttcag
     2281 cgccgcgccg tagtacttcg gggcggcgcc gaagcactac gctgccgcgc cgaactactc
     2341 caaaacgact ttgaagcact tccctgccgc agggaagcac tccgccgcgg cgccgtagta
     2401 cttcgctgcc gctcccgaac actccaatgc agcctccgag ggagcggtgg tgcctccccc
     2461 cctgggaggg agggcggtag ggagcggggg aggttaggag gggggttttt ctccgccccc
     2521 gcgacctcca acctacccac aagaatcttc catttccctc ttgacgcgcc gccagtcgca
     2581 ggtatagcct tcgaggcgga cgtaagccgt acaacagaag gaggtggcag ggggagagaa
     2641 cgaagccgcg gtttcaaagc acagccgtac aggaccgctg tatctccggg gtgagcgtgt
     2701 ggctcatccg gggttccggg gtccaagcgg ccaagggagg gagcggctaa ctcgcctccc
     2761 gaggccttgc cgagcagagg aggtgcctca tgcccgacca cgatgatcgt acgccttcgc
     2821 gcgtcgatct gtcccgtccc accgaatcca cccgccgtca tctctatgac ctggtggtcc
     2881 acgtctactt ccatcccacg cctgaagagg tggagaaggc caaagcggat cccggcaatc
     2941 attgggtgcc ggactacggt cccgacgcgg cgcgcctcac cgtcttctat gcctacggcc
     3001 gctggttcgc ggtctgggag gcgctggagg agaaggagac cgggctgccc gagtggcggc
     3061 gctggtcggt ggtgcggatc gtcgaggacc gggacccgtc gagcgcgacg ggtgtgacct
     3121 ttctggaggt ctgacgccgg ctgcccggcg ggtcccgcgg cgcggggccc gccgttttcg
     3181 agcagagtgg cgggaagatc aagctagcaa gggaactcac cactctggca tggaaatgga
     3241 ccggtggggc aatagtatgt cttcgggcac agggctgtag ccccgcctgt caccccagga
     3301 agcagcgccg ctagtgtctc cttcgccaga accagcttct tcaccttctt cgtcttcttc
     3361 atcgccactc cttagtagat gcgcgccacc tggcgccagg acattgtagc ctgccgggct
     3421 aggagcgatc cgcctccccc tctcccgggg gccggggggc ggcggcctca aacacactac
     3481 aatggcgccc atgcatctgg aggacggtct cctttccggc gtgccggatc ccgaggacct
     3541 gggggtcgag ccgtcgctgc ggccgcagcg gctcgacgag tacatcggcc aacgcaagat
     3601 cctggacaac ctgcgggtgt tcatccgcgc cgcccgggag cgccgcgagt cgctcgacca
     3661 tgtcctcctg ttcgggcccc cgggcctggg caagaccacc ctcgcccaca tcatcgccac
     3721 cgagatgcag gcgccgctgc gcctcaccgc cgggccggtc ctcgagcgcg ccggcgacct
     3781 cgcggccatc ctcaccaacc tggagcccgg cgaggtgctg ttcatcgacg agatccaccg
     3841 cctcggcccc accgtcgagg agatcctcta cccggcgctg gaggatttcc ggctcgacct
     3901 cgtcatcggc cagggcccgg cggcgcgcac ggtcaagatc gacctcccgc gcttcaccct
     3961 ggtgggcgcc accacccgcg ccggcctcat cagcgccccc ctgcgcgccc gcttcggcat
     4021 cgtccaccgg ctggacttct accggccgga ggagctgaag ctgatcgtcg agcgctcgtc
     4081 gcgcctgctg tcgatcggta tcgacccggc cggggcgctg gagatcgccc gccgcagccg
     4141 cggcaccccg cgcgtcgcca accggctgct gcggcgggtg cgcgacttcg cccaggtgga
     4201 gcgcgccgac cgcatcgagc tggagatcgc ccgcaagtcg ctcgccttgc tggaggtcga
     4261 cgagtacggt ttcgaggagg tcgaccgcaa gatcctgggg accctcatcg agcactacgg
     4321 cggcggaccg gccggcctca aggcgctggc cgcggccgtc ggcgaggaca gcggcaccct
     4381 ggaggacctc tacgagccct atctgattca ggagggcttc ctccagcgta ccccgcgggg
     4441 ccgggtcgcc acccgccgcg cctacgagca cctgggctat cccccccgcc ccggctcgat
     4501 gggcaccctg ttctgatcgg ccgtgggtca ggtcgcccgt gctcggctcc ttcgtgtgcg
     4561 ctaccccctc cctccccacc ccgctgccag tcgcctgcgc cgggcggcct gacccactcc
     4621 gtccgtgccg accatgcgcc gggccgcgct gatctacaac ccccgcgcgg ggcgccagcg
     4681 gcaggccgaa gggctggacg gcatcgtcgc cgccctgcgg tctggggggt gctttgccga
     4741 gccggtgccg acgcgcggcc cgggcgatgc caccaccctc gcccgcgagc tcgccgccag
     4801 gggtgacgtc gaagcggtct ttgcgttcgg cggcgacggc accgtccgcg aggtagcggc
     4861 cgggctcctg ggcagcggcg tcccgctggg catcctgccg ggcggcacgg tcaacctgct
     4921 ggcgatggcc ctcgggctcc cccccgaccc ggtggccgcc gccgccgtgc tcgccggcgc
     4981 gccggcccgg cccatggacg tcgggctcgc cggcgccagc cccttcttga tgatggtctc
     5041 cgccggcctc gacgccagcg tcctggccgc cgtggacccg cgcctcaagc tccacttcgg
     5101 ccagggggcg gtcctcggcc acggcctggt cgagtggtgg cgctacgggt atcctcccct
     5161 gcaggtggtg gccgacggcg agcggctgga ggcgaccttc gcggcggtgt ccaatatccc
     5221 gtactatgcc ggcgccttcc acctggcccc cggggccagg ctggacgacc ggcggctcga
     5281 cctggtcctc ttccgcggca ccggccgcgt ggcgaccgtc ctcttcgccc tggacctgct
     5341 gcgcgccgcc cacgtgcgcc gccgcgacgt cctcatccgg ccggtggagg aggtggtctt
     5401 caccggcccc gccggcgccg ccacccaggt ggacggcgac ctgtgccagg agacggtgcc
     5461 gctgaccgtc cggctggcgc ccgagacgat cccggtcctg atgccggccg tctgatccca
     5521 tg
//
LOCUS       DASPJI010000632         5324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_40559, whole genome shotgun sequence.
ACCESSION   DASPJI010000632 DASPJI010000000
VERSION     DASPJI010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40559"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VEL74_17725"
     CDS             <1..287
                     /locus_tag="VEL74_17725"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG64422.1"
                     /translation="LLHERGEVGGAEMHYRLALAARPDDPIAAYDLGVALQDQSRLEE
                     AAQAYQMALTADAANADAHYNLAAVYEAMGQRQAAFRHFKSYRALTGKAS"
     gene            960..3143
                     /locus_tag="VEL74_17730"
     CDS             960..3143
                     /locus_tag="VEL74_17730"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HYG64423.1"
                     /translation="MNRKTLLVLVVACLALAPGGRALAVEGKWTPEQILELDPKWLRE
                     QGLEIPPERLWGRDGAGLLDAAVKIDGCSAGFISADGLLVTNHHCAFGILQQHSTPER
                     DLITGGFLAANRAGELPGNGTRATIPHRTEDVTAQVEAAVPAGADDLARFRAIERKTK
                     ELVAACEAQPNRRCQVSAFDGGVRYLLIESLELPDVRLVYAPPRAVGEYGGEVDNWSW
                     PRHTGDFALLRVYTGPGGAPARPAEGNLPYKPRHWFPVAPKGVGPGDFVLVAGYPGLT
                     YRSLTEAEMRERAELWFPRRAELYRTWIGLMEAASEKEEAARIALADRAKTLANREKN
                     ARGQVAGIRRGRILERKQESERQVLAWAAGRADQQGAVAAHRELDQLIQAERRTWDRD
                     FLLEQIRQGPKPLDMALTLVRWAGEKAKPDLEREPEYMERNRDRLADNLRNDQKRFHL
                     PTDEAILADLLGRFAALPEGSRVPAVEALLAGARTPEAVRAKAASLLAGTKVTGLAER
                     TEMSGESEEQLRARRDPLLDFAFAFDRELRAWKERDDRHKGAVSRLRPAWQRAVIAHA
                     GKPVAPDANGTLRLSLAHVQGYEPRDAVRMEPQTTVAGLAEKHTGEEPFDAPDALLAA
                     APSAPRSRWADPKLGDVPVAFLADADTTGGNSGSPVVNGRGELVGVNFDRVWENVAND
                     FGYNPDVARNISVDVRFLLWTLETTHGEAARPLLAEMGAAGPAPR"
     gene            3282..>5324
                     /locus_tag="VEL74_17735"
     CDS             3282..>5324
                     /locus_tag="VEL74_17735"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYG64424.1"
                     /translation="MRDRDWEQRRSPWWSLADRSIRTKLLAAFLFVAILPLGLLTLHN
                     DRASRETLTEVANRSLLDAARQTATSLDAFLEAHLDIVATEARQPVLAQHLASLEEGG
                     PGASQISTLLATFQGRMPEAIASYALLDRRGDNVADTDPRGVGGSEAGNDYFQKVMKA
                     EGPTHVSRVEFSSDGRPYLTLSAPVRDPGGQAVGVLRARYHASILRDRIAPSEQEKLT
                     GPPESFAMLFDERRIVLAHGAIPHFGSEDIRFRTVATAESLVEAMMAQRRLPRKPKDQ
                     IKSLSLDLPDLENGLTGASSQAPYFTARLKRDDPTLYAAAVAKTSREQWTVAFLQPRD
                     AYLQPANEQTRENLLLGLILTGAVAALALAVAERLARPITRLTAVARQVAGGELDAQA
                     PVTSQDEAGQLAAAFNTMTAQLRRLIQSLQDRIAERERAEESLSTTLNSIGDAVIATD
                     THGRVVRMNPVAERLTGWPMEMAAGQPLHDVFRIVNEDTRLPVVSPVDQVLASGGVVG
                     LANHTVLLARGGSERPIADSGAPIRGREGRIEGVVLVFRDVTEERRAEEALRREHALL
                     RQSEERFRLVIDQMPAFLWSTDRDLRFTLSLGAGLSALGLKPNETVGQTLFDYYQTDD
                     PSFPAIAAAYRALEGESVSFDLEWEGSVFQCYVEPLRDSEGAIVGAIGVPLDISERK"
BASE COUNT          819 a         2053 c         1779 g          673 t
ORIGIN      
        1 ggctgctgca cgagcgcggc gaggtgggag gcgccgagat gcactatcgg ctcgccctcg
       61 ccgcccgccc ggacgacccc atcgccgcct acgacctggg agtcgccctg caggaccaga
      121 gccggttgga ggaggccgcc caggcctacc agatggccct caccgccgac gccgccaacg
      181 ccgacgccca ctacaatctg gccgcggtat acgaagccat gggccagcgc caggccgcct
      241 tccgtcactt caagtcctac cgggcgctga cgggcaaggc gtcgtagggc ccctcaagcc
      301 cccggcgtcg cgccgaggtg ttccaaggtt acaccgtagc gtttcccggc agaagggaaa
      361 cgctacgagg ccattcctca gcactacggc gcggctccat agcgtttccc gattgaaggg
      421 aaacactacg acgccatccc tgaacgctac agcgccgctc ctcaacacta cagcgcgact
      481 ccgtagcgtt tcccggcaga agggaaacac tacgacgcca tccctgaacg ctacggtgcg
      541 gctcctcaac actacagcgc gactccgtag cgtttcccga ctgaagggaa acgctacgac
      601 gccattcctg aacgctacag cgtggcgcct caacactacg gcgcggctcc gtagcgtttc
      661 ccgaccgaag ggaaacgcta cgacgccgtt cctgaacact tcggcgcggc tcctcagcgt
      721 ttccctgccg cggggaagca ctacggaatc gtcccgaagc actgagggat ggctcctcaa
      781 ggcttctccg ccgccgggaa accgttcgaa cgaaaagcgc cccgtcccgc tggcgcggag
      841 acggggcgct cgagaaaggt catgcccgtc gggagaggtg gccaggggtg agggcccgaa
      901 ggcctccctt ccttatatag atagggccac cccctcgatt ccggctagac taccgggcca
      961 tgaaccggaa gactttgctc gtactcgttg tcgcctgcct ggccctggcg ccgggaggga
     1021 gggcgctggc ggtggaagga aagtggaccc ccgagcagat cctggagctc gatcccaagt
     1081 ggctgcgcga gcaggggctc gagatcccgc ccgagcggct gtggggccgc gacggcgcgg
     1141 ggctcctcga cgccgcggtc aagatcgacg gctgctcggc cggcttcatc tccgccgacg
     1201 gcctgctcgt caccaaccac cactgcgcct tcggtatcct gcagcagcac tcgacccccg
     1261 agcgcgacct gatcaccggc ggcttcctcg ccgccaaccg ggccggggag ctgcccggca
     1321 acggcacccg ggcgaccatc ccccaccgca ccgaggacgt caccgcccag gtggaggccg
     1381 ccgtgcccgc cggcgccgac gacctcgccc gcttccgcgc catcgaacgc aagaccaagg
     1441 agttggtggc cgcctgcgag gcgcagccca accgccgctg ccaggtctcg gccttcgacg
     1501 gcggcgtccg ctatctgctg atcgagagcc tggagctgcc cgacgtgcgg ctggtctacg
     1561 cgcccccccg cgccgtcggc gagtacggcg gcgaggtcga caactggagc tggccccggc
     1621 acaccggcga cttcgccctg ctgcgcgtct acaccgggcc cggcggcgcg ccggcccgcc
     1681 cggcggaggg caacctcccc tacaagcccc ggcactggtt cccggtcgcg cccaaggggg
     1741 tgggccccgg cgacttcgtg ctggtcgccg gctaccccgg cctcacctac cggtcgctga
     1801 ccgaggccga gatgcgcgag cgcgccgagc tctggttccc ccgccgcgcc gagctgtacc
     1861 gcacctggat cggcctcatg gaggccgcct ccgagaagga ggaggccgcc cgcatcgccc
     1921 tcgccgaccg cgcgaagacc ctcgccaacc gggagaagaa cgcccgcggc caggtggcgg
     1981 gcatccggcg cggccggatc ctcgagcgca agcaggagtc cgagcgccag gtcctcgcct
     2041 gggccgccgg ccgcgccgac cagcagggcg cggtggccgc ccaccgcgag ctcgaccagc
     2101 tcatccaggc ggagcgccgc acctgggacc gcgacttcct gctcgagcag atccgccagg
     2161 gccccaagcc gctcgacatg gcgctcaccc tcgtccgctg ggccggcgag aaggccaagc
     2221 ccgacctcga gcgcgagccc gagtacatgg agcgcaaccg cgaccggctg gcggacaacc
     2281 tgcgcaacga ccagaagcgc ttccatctcc ccaccgacga ggcgatcctc gccgacctcc
     2341 tcggccgctt cgccgccctg ccggagggga gccgcgtgcc cgccgtcgag gccctgctcg
     2401 ccggcgcccg gacgcccgag gcggtgcggg ccaaggccgc ctcgctgctc gccggcacga
     2461 aggtcaccgg cctcgcggag cggacggaga tgtccggcga gagcgaggag cagctccgcg
     2521 cccggcgcga cccgctgctc gacttcgcct tcgccttcga ccgcgagctg cgcgcctgga
     2581 aggagcggga cgaccgccac aagggggcgg tgtcgcgcct gcgcccggcc tggcagcgcg
     2641 cggtgatcgc ccacgccggc aagccggtgg cgccggacgc caacggcacg ctgcggctct
     2701 ccctcgccca cgtccagggg tacgagccca gggacgcggt gcgcatggag ccgcagacga
     2761 cggtcgccgg cctcgccgag aagcacaccg gcgaggagcc gttcgacgcg ccggacgccc
     2821 tcctcgccgc ggcgccgtcg gcgccgcgca gccgctgggc cgacccgaag ctcggcgacg
     2881 tgccggtcgc cttcctggcc gacgccgaca ccaccggcgg caactcgggg agcccggtgg
     2941 tcaacggccg cggcgagctg gtcggtgtca acttcgaccg ggtctgggag aacgtggcca
     3001 acgacttcgg ctacaacccg gacgtcgccc gcaacatcag cgtcgacgtc cgcttccttc
     3061 tgtggaccct ggagaccacc cacggcgagg cggcgcgccc gctcctcgcc gagatggggg
     3121 cggccggccc ggcgccccgc tgacccgccc cctcccaccc gccggccggg gtttcccggc
     3181 tggctggttc ctgctacgat ttcggcgctc ttgcaagcgt tcaagtcacc gcgagcgccg
     3241 cccccggcgg ccggatcggt ggagaggcaa agcgtcggcc gatgcgtgat cgagactggg
     3301 aacaacgccg cagtccatgg tggtcgctcg ccgaccgctc gatccgtacc aagctgctcg
     3361 ccgctttcct cttcgtcgcc atcctccccc tggggctgct gaccctccac aacgaccgcg
     3421 cctcgcgcga gacgctcacc gaggtggcca accggtccct cctcgacgcg gcgcggcaga
     3481 cggcgaccag cctggacgcg ttcctcgaag cccatctcga catcgtggcc accgaggcgc
     3541 gccagccggt gctcgcccag cacctcgcca gcctggagga gggggggccc ggcgccagcc
     3601 agatctccac gctgctcgcc accttccagg gacgcatgcc ggaggccatc gcctcctacg
     3661 cgctgctcga ccgccgcggc gacaacgtgg ccgacaccga tccgcgcgga gtcggcgggt
     3721 ccgaggccgg caacgactac ttccagaagg tgatgaaagc ggagggcccg acgcacgtct
     3781 ccagggtgga gttcagctcc gacggcaggc cctacctgac gctgagcgcg ccggtccggg
     3841 accccggcgg ccaggcggtg ggcgtcctgc gggcgcgcta tcacgccagc atcctccggg
     3901 accggatcgc ccccagcgag caggaaaagc tcaccggtcc gccggagtcg ttcgccatgc
     3961 tcttcgacga gcgccggatc gtcctcgccc acggggccat cccccacttc gggtcggagg
     4021 acatccgttt ccggaccgtg gcgaccgccg aaagcctggt cgaggccatg atggcccaac
     4081 ggcggctgcc ccggaagccc aaggatcaga tcaaatccct gtccctcgac cttcccgacc
     4141 tcgagaacgg gctgaccggg gcctccagcc aggcacccta cttcaccgcc cgcctcaaac
     4201 gggacgaccc caccctctat gccgccgccg tcgcgaagac gagccgcgag cagtggaccg
     4261 tggccttcct gcagccgcgg gacgcctacc tccagccggc caacgagcag acgcgcgaga
     4321 acctgctgct cggcctgatc ctcaccggcg cggtggcggc gctcgccctc gccgtcgccg
     4381 agcgcttggc gcggccgatc acccggctga ccgcggtggc gcggcaggtg gccggcggcg
     4441 agctcgacgc gcaggcgccg gtgacgtcac aggacgaggc cggccagctc gccgccgcct
     4501 tcaacaccat gaccgcgcag ctgcggcggc tcatccagag cctccaggac cggatcgccg
     4561 agcgcgagcg cgccgaggag agcctgtcga ccaccctcaa ctccatcggc gacgcggtga
     4621 tcgccaccga cacccacggg cgcgtggtgc gcatgaaccc ggtggccgag cggctgaccg
     4681 gctggccgat ggagatggcg gccgggcaac cgctccacga cgtcttccgg atcgtcaacg
     4741 aggacacccg cctgccggtg gtcagcccgg tcgaccaggt gctcgcgagc ggcggcgtgg
     4801 tggggctcgc caaccacacg gtgctcctcg cccgcggcgg cagcgagcgc ccgatcgccg
     4861 acagcggcgc ccccatccgc ggccgggagg ggcggatcga gggggtggtc ctggtcttcc
     4921 gggacgtcac cgaggagcgc cgcgccgagg aggccctgcg tcgcgagcac gccctgctgc
     4981 gccagagcga ggagcgcttc cggctggtga tcgaccagat gccggcgttc ctgtggagca
     5041 ccgaccgcga cctccgcttt accctgtcgc tcggcgccgg cctctccgcc ctcggcctca
     5101 agcccaacga gaccgtgggc cagaccctct tcgactacta ccagaccgac gacccgagct
     5161 tccccgccat cgccgccgcc taccgggcgc tcgaagggga gtccgtctcc ttcgacctgg
     5221 agtgggaggg gagcgtcttc cagtgctacg tcgagccgtt gcgcgacagc gagggggcca
     5281 tcgtcggcgc catcggcgtg ccgctcgaca tcagcgagcg caag
//
LOCUS       DASPJI010000633         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_439461, whole genome shotgun sequence.
ACCESSION   DASPJI010000633 DASPJI010000000
VERSION     DASPJI010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439461"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2102)
                     /locus_tag="VEL74_17740"
     CDS             complement(<1..2102)
                     /locus_tag="VEL74_17740"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG64425.1"
                     /translation="MSDLAARISALSPEKQRLLARIRQDQKGETARPKTLTEPFSLVS
                     PADRERLPPGLEDAYPLSMVQLGMLFHMQLTPDAPTPTYHNVNSFNLRVPFDAAALEE
                     AAQILVARHPILRTSFDLTGYSEPLQLVHRSASLPIAVEDLRHLPAEEQARAIESFIL
                     EENRRLVDLTRPPLIRFHVHRRSDRGIQFTLTEPHAISDGWSTMSNLSEVFSAYFALL
                     SGEPVPVEPPISSTYRDFVFLERQVLRSEEARQFWRRMLGDWEMPQLPRWRSRAAGGE
                     GFGGEDRKPTFGMPTRLVEGLERLARSSGLPFKSLLLAVHLKVMGMVTGREDAVTGIT
                     SHGRPEDRDGERVRGLFLNTIPLRVSLAGGSWLDLARRALQAETGTLPFRRYPLAALQ
                     QLCGGRELFEVNFTYLHFHNVQGVVGNEKLGFAFDGHSDLSVTNFPLAVTLIRNPVAP
                     AGLTLTLERNDPGISPAQVRALYGWYERALAAMVADPGADHGSCALLSPAERQQVLAE
                     WNDTAVSFDTDALIHEAFERRAAAAPEALAVVGEDGRRTYGEVEARANRLARHLAGLG
                     VGPGSMVGIYLERSVEMVPALLAVLKAGGAYVPLSDSFPPARMEWILASLDVRHLLTQ
                     TPRLPVARALQTAVPGLAHLVCVDMDEADGLPSTPLGRRAGAEDLAYVIFTSGSTGRP
                     KGVAVRHRPVANLVEWALR"
BASE COUNT          327 a          774 c          831 g          296 t
ORIGIN      
        1 cggagggccc actccaccag gttggccacg gggcggtggc gcacggcgac ccccttggga
       61 cggccggtgg agccggaggt gaagatgacg taggccaggt cttcggcacc ggcgcgccgg
      121 ccgagggggg tggaggggag accgtccgcc tcgtccatat ccacgcatac caggtgcgcg
      181 agcccgggga cggcggtctg gagcgcccgc gccaccggca ggcgcggcgt ctgggtcagc
      241 aggtggcgca cgtccaggga ggcgaggatc cactccatgc gcgccggcgg gaagctgtcc
      301 gagaggggca cataggcgcc gcccgccttg agcacggcga ggagggccgg caccatttcc
      361 accgagcgct cgagatagat ccccaccatc gaccccgggc cgacgcccag gccggcgaga
      421 tggcgggcga ggcggttcgc ccgcgcctcc acctcgccgt aggtgcgccg gccgtcctcc
      481 cccaccacgg ccagggcctc tggcgccgcc gcagcccgcc gctcgaaagc ctcgtggatg
      541 agcgcgtccg tatcgaacga caccgccgtg tcgttccact ccgccagaac ctgctggcgc
      601 tcggccggcg atagcagcgc gcaggagccg tgatcggcgc ccgggtccgc caccatggcg
      661 gcgagggccc gctcgtacca gccgtacaag gcgcgcacct gggccgggga gatgcccgga
      721 tcgttgcgct ccagcgtcag ggtcagcccg gccggcgcca ccgggttgcg gatcaaggtg
      781 acggcgagcg ggaagttggt caccgacagg tcgctgtggc cgtcaaaggc gaagcccagc
      841 ttctcgttgc ccaccacccc ctggacgttg tggaagtgga ggtaggtgaa gttgacctcg
      901 aacagctccc tcccaccgca gagctgctgc agggcggcga gcgggtagcg ccggaacggc
      961 agggtcccgg tctcggcctg gagggcccgc cgagccaggt cgagccagga gccgccggcg
     1021 aggctcactc gcagcgggat ggtgttgagg aagagcccgc gcacccgctc gccgtcccgg
     1081 tcctcgggcc gcccgtggct ggtgatgccg gtgaccgcgt cctcgcgtcc ggtcaccatc
     1141 cccatcacct tgaggtggac ggcgaggagc aggctcttga acggcaggcc ggaggagcgg
     1201 gcgaggcgct ccagcccctc caccaggcgg gtgggcatgc cgaaggtcgg cttgcggtcc
     1261 tcgccgccga agccctcccc gccggcagcc ctggagcgcc agcggggcag ctgcggcatc
     1321 tcccaatcgc ccagcatgcg gcgccagaac tggcgcgcct cctccgaccg cagcacctgg
     1381 cgttcgagga agacgaagtc gcggtaggtc gaggagatcg gcggctccac cggcaccggc
     1441 tcgccgctca gcagggcgaa gtaggcgctg aagacctcgg agaggttgga catggtgctc
     1501 cagccgtccg aaatggcgtg cggctcggtg agggtgaact ggatgccgcg gtccgatcgg
     1561 cggtggacgt gaaaacggat cagtggcggc ctggtgaggt ccaccagccg ccggttctcc
     1621 tccaggatga agctctcgat ggcccgcgcc tgctcctccg ccgggaggtg ccggaggtcc
     1681 tccaccgcga tgggcaggga ggccgagcgg tggacgagct gcagcggctc gctgtagccg
     1741 gtgaggtcga acgacgtgcg caggatcggg tgacgggcca ccaggatctg cgccgcctcc
     1801 tccagcgctg ccgcgtcgaa cggcacccgc aggttgaagc tgttgacgtt gtggtacgtc
     1861 ggcgtcggcg cgtccggagt gagctgcatg tggaacagca tgccgagctg caccatggac
     1921 agcggatagg cgtcctccaa gcccggcggc agccgctcgc ggtcggcggg cgacaccagg
     1981 gagaacggct cggtcagcgt cttgggccgg gcggtctccc ccttctggtc ctggcggatg
     2041 cgcgccagca ggcgctgctt ctcgggcgag agggcggaga tccgggcggc gagatcgctc
     2101 atcaggtgct ctccccgtcg agcaccgcca tccgctcctg cgcctcctcg tcggagaggg
     2161 cctcgagctc ggcgaccagg cgctcgagct cctcgtcacc gagccgggcg gccgccgtct
     2221 ccgccggc
//
LOCUS       DASPJI010000634         7630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_607990, whole genome shotgun sequence.
ACCESSION   DASPJI010000634 DASPJI010000000
VERSION     DASPJI010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607990"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            366..1343
                     /locus_tag="VEL74_17745"
     CDS             366..1343
                     /locus_tag="VEL74_17745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYG64426.1"
                     /translation="MAASPEFSVIIPTHNRLEVLAEVIRALEFQDGAPDFEVVVVDDG
                     SSDGTADWLRNRSFQVNLRVFTQENRGPAAARNTGVAVAAGKWVAFLGDDTVPSPGWL
                     ATHRAAHRRRGDDSHLAVIGYTAWHSRMQLTPFLRYINEHGLQFGYALIRDKEDVPFN
                     FFYTSNLSLPRDLLLAEPFDLRFPYAAWEDIEVSYRLKKRGMRLVYEPGATVAHDHPT
                     DLARFASRQEKAGYCAVVFYRLHPELGPFLGLGPGGPPPVPPERRQRWLEWLVRALQR
                     GPFHLPGFWEEVLRYHYIKGLHRAWSDQGAELEKENVHDSSETGGQAAS"
     gene            1306..2460
                     /locus_tag="VEL74_17750"
     CDS             1306..2460
                     /locus_tag="VEL74_17750"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HYG64427.1"
                     /translation="MTLQRLAAKRLLEGGAVAVTALLLAGPVLASPDQSRQRPSDSGS
                     GGRTAIPNNPSPSAPPSGGSGVSGDSGRSHDSSPPPRTAQPAPGAGNERPDRQPSHRR
                     GGGGGGGRGGYYYGDGWHRPWYGGWGWWGYGWHGYGYPPYYGPGGYPYYGAYREDVLG
                     ALDLDVSPGRAQVYLDGRYLGTVDAYDGFPRYLWLEKGTYDLVLFLDGYRTVARQVSV
                     YPGTVLGINDRLEEGESTPPEQMVTPRSTERRDARIREERERREEIERGDDWRDRVRD
                     ERRRRDDDGYDRRGDDDGRDDRGEWGDRGDRGEESRHSNVRLDVEPRDASVYLDGRFV
                     GTGEDLRGLSVNPGRHQLAVVRPGYESKEQEFEVTAGEELVLEVELEEND"
     gene            complement(2515..2958)
                     /locus_tag="VEL74_17755"
     CDS             complement(2515..2958)
                     /locus_tag="VEL74_17755"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP-like transmembrane domain"
                     /protein_id="HYG64428.1"
                     /translation="MALSSLGNGLDELGTTNRKNPFSRLNTRFNAGSRNVGNVERWAS
                     VLGGAALAALALRRRSKGAMGLAALAGAPLLWRGATGHCAVYQKLGIDRGGQTGLSEN
                     TQLATLDTLDPHRFDFDEGRNKPLDGTTEARPVTTTDRQPIGQNI"
     gene            complement(3186..6077)
                     /locus_tag="VEL74_17760"
     CDS             complement(3186..6077)
                     /locus_tag="VEL74_17760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HYG64429.1"
                     /translation="MMRETYNSFRRDLAGMPPARRRVVIGVLTTFGLVLVLGLAGAVY
                     LWDLARRFPQAPFKQPSRLYGEATRLAPGAGIGIDDIVAELESQGYREAEEESEKGEA
                     DEAEGKDEGESAAEGGEGDAGRPLPPGTFRRQEDRLAVDLRSSPTTEGPAPGGLLEVT
                     FRGRRIGEIRQAGRVVDEAALPPPLLASYYDDEVQERRPVTLDQVPEHVIQAILAAED
                     DGFYTHPGVSPTGVARALWVNLRGEGEVQGGSTITQQLVKNVYLSSERTLSRKAKEAL
                     IAMMLEIRHGKKAILEAYLNEIYWGASSSVNLIGLGAASYAYYGKEPRELTLSEAATL
                     AGMIQAPANYLPTQHPEEARERRDWVLQRMDELDWITPEQRQRAAATPVVARPQKLVT
                     RPLAPYFAEFAKQEATERFDIEDIGGEGYLLFSSLRWRDQKEAQRALERGLASLDPRG
                     GKNGKDPLQGALVSVDPRDGAILAYVGGRDYADSQFDRVSTARRQAGSAFKPVVYAAA
                     FAESVATPVTLLRDSPITVKVDNKPWRPQNYDRGFQGSVTARTALERSLNIPTIRLAL
                     QVGLHRVVDLSRDMGIEGEMDTVPALALGTFEVSPRELAEVYSVFANDGLRPRVHGLK
                     LVLDREGQEVDGEELESPQRVLSPQVAYLVTSGLQGVLDRGTAAAARGWGVQGRLAGK
                     TGTTNDRRDNWFAGYSPDRVTVVWVGYDDNRRTRFSGSTAALPVWSRFTMAVRPAGGY
                     KDFARPQGIVQLAIDPTTGQLATDACPYVVTDVFPDWKVPTEPCHRHGHPSGGRIYAD
                     AGGYYDPYATDPDPYGQYSEEDPYAGEPVDPYLLDTYIDQEGPRPVITNQPAALPPRT
                     VRVNPLEPVGVDPAEIDTEAEEGVAVGEGEEDGSYIRPSSQRPAPPEPPLAGTAQPAP
                     PEAAAADTPASEGEDETEIDVSVGTVEPSPTATPPAPPPAPPVGTPVGL"
     gene            6258..6563
                     /locus_tag="VEL74_17765"
     CDS             6258..6563
                     /locus_tag="VEL74_17765"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HYG64430.1"
                     /translation="MSDVEAKSALKGLELRQLYARLDLAGWTLISEHHLEKEYRFNDF
                     REALAFVNRLGEVAEEEGHHPDIFLTWGKVKLTLWTHSVGGLSEKDFILAERADRLL"
     gene            complement(6605..>7630)
                     /gene="glgP"
                     /locus_tag="VEL74_17770"
     CDS             complement(6605..>7630)
                     /gene="glgP"
                     /locus_tag="VEL74_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HYG64431.1"
                     /translation="VSALHGRVSRKMWHSLYPGRTEEEVPIGHITNGVHVRTWLAADM
                     HDLMARYIGPSWLESLSRPELWSRIAKVPDAELWEVHQVLKARLFTFARRRLAERRER
                     LGMPEPENEPLAPDALTLGFARRFATYKRADLLFQDLDRLDRLVNDPERPLQIVYAGK
                     AHPRDTGGKALAQRVANLEHDPRFAGRIVFIENYSMHVARQLVQGVDAWLNTPRRPLE
                     ACGTSGQKCILNGVLNVSILDGWWAEGYDGANGFAVGGEAVHANPDVQDERDALALFE
                     TLERQVVPLYYQRDADGLPYGWIARVKRAIRTMAWRYNADRMVMDYVRESYVPAAGGD
                     SCRMPSS"
BASE COUNT         1054 a         2632 c         2766 g         1178 t
ORIGIN      
        1 gcatcgggcg cggcgagggg ggcgggttgg agttctatga ggtgtagggg cggaggggag
       61 tagcagagta gacgagcagt ggtcaggggc ggcgtcagcc gcccccgact acctcccaaa
      121 agcaaccccc cacctcggtc ctcccccgct cccttccacg ccacctccca gggggggagg
      181 aagcccgccc ttccccgaga tcgcgagcta gcgtcgcctg tatgcaccga gagcccccgc
      241 agggggcggc cctgttctca gaccgggccg tcagccccgg gggtggcagg cggagcgctc
      301 tgctcggccc tcgccccgcc cgcccgcgcg gcgctggaag tcccctcctc gggtaagctg
      361 tgagcatggc cgcttccccc gagttctccg tcatcatccc cacccacaac cgcctggagg
      421 tgctggcgga ggtgatccgg gccctcgagt tccaggacgg ggctcccgac ttcgaggtcg
      481 tcgtggtcga cgacggctcc agcgacggca cggccgactg gctgcgcaac cggagcttcc
      541 aggtcaacct gcgcgtcttc acccaggaga accgcggacc ggccgccgcc aggaacaccg
      601 gcgtcgccgt cgccgccgga aaatgggtgg cctttctggg ggacgacacc gtcccttctc
      661 cgggatggtt ggcgactcat cgcgcggccc accggaggcg cggcgacgac tcccatctgg
      721 cggtcatcgg ctacaccgcg tggcactcgc ggatgcagct cacacccttc ctgcgctaca
      781 tcaacgagca cggactgcag ttcggatatg cgttgatccg cgacaaggaa gacgtgccat
      841 tcaacttttt ttacacgtcc aacctgtcgc tcccgcgcga tctgctcctc gccgagccct
      901 tcgatcttcg ctttccctat gcggcatggg aagatatcga ggtctcctat cgcctcaaga
      961 agcgtggaat gcggctggtc tacgagcctg gagccacggt cgcccatgat cacccgacgg
     1021 atctcgcccg gttcgcgtcg cggcaggaga aggcgggcta ctgcgcggtg gtcttctacc
     1081 ggctccaccc ggagctcgga cccttcctcg ggctcggtcc gggagggccg ccgccggtgc
     1141 ctccggagcg gcgccagagg tggctggaat ggctggttcg tgctttgcaa agggggccgt
     1201 tccatctccc cggtttttgg gaagaggtgc ttcggtacca ctacataaag ggtcttcacc
     1261 gcgcctggtc agaccagggc gcggagctgg aaaaggagaa tgtccatgac tcttcagaga
     1321 ctggcggcca agcggcttct tgagggtggc gcggtggctg tgacggccct gctgctcgcg
     1381 ggaccggtcc tggcatcgcc ggatcaatcg cgtcagcgcc cctcggattc tggatcgggt
     1441 ggccggacgg ccatacccaa caacccgtca ccctcggcgc ctccgagcgg cggctccggc
     1501 gtgtccggcg acagcggccg gtcccacgac tcgtcgccgc cgccgcggac ggcccagccg
     1561 gcccccggcg ccggcaacga gaggccggac cggcagccgt cccatcgccg tggtggcggc
     1621 ggtggcggcg gccgcggcgg ctactactac ggcgacggct ggcaccggcc ctggtacggc
     1681 ggttggggct ggtggggcta cggctggcac ggctacggct atcccccgta ctacggcccg
     1741 ggcggctacc cctactacgg cgcctaccgc gaggacgtcc tcggggcgct ggacctcgac
     1801 gtcagccccg gccgcgccca ggtctacctc gacggccgct acctcggcac ggtggacgcc
     1861 tacgacggct tcccccgcta cctctggctc gagaagggga cctacgactt ggtcctcttc
     1921 ctcgacggct accgtaccgt cgcgcgtcag gtctccgtct atccgggcac ggtcctcggc
     1981 atcaacgacc ggctggagga gggggagtcg acgccgccgg agcagatggt gaccccgcgg
     2041 tccaccgagc ggcgtgacgc ccgtatccgc gaggagcgcg agcggcgcga ggagatcgag
     2101 cgcggcgacg actggcgcga ccgcgtccgc gacgagcggc ggcgccggga cgacgacggc
     2161 tacgaccgcc gcggcgatga cgacggccgg gatgatcggg gggagtgggg ggaccgtggg
     2221 gaccgcgggg aggagtcgcg gcacagcaac gtgcgcctcg acgtcgagcc ccgggacgcc
     2281 tcggtctacc tcgacggccg cttcgtcggc accggcgagg acctgcgcgg gctgtcggtc
     2341 aatcccggcc gccatcagct cgcggtggtc cggcccggct acgagagcaa ggagcaggag
     2401 ttcgaggtga ccgccggcga ggagctggtg ctggaagtgg agctggaaga gaacgactag
     2461 cggatcgtcg atggacggcc cctctcccgg ggtgggggag gggccggttg ttctctagat
     2521 gttctggccg atcggctgcc ggtcggtcgt ggtgaccgga cgggcctcgg tcgtgccgtc
     2581 gagaggcttg ttccggccct catcgaagtc gaagcggtgg gggtccaggg tgtcgagggt
     2641 ggcgagctgg gtgttctcgc tcaggccggt ctggccgccc cggtcgatgc ccagcttctg
     2701 atagaccgcg cagtgaccgg tggcgccacg ccacagcaac ggcgcgcccg ccagggccgc
     2761 caggcccatc gcacccttgg agcggcgccg cagggccaga gcggcgagag ccgccccacc
     2821 cagcaccgag gcccagcgct cgacgttacc tacgttcctg ctgccggcgt tgaaccgggt
     2881 gttgagacgg ctgaaggggt tctttcggtt cgtcgtcccg agctcgtcga gcccgttgcc
     2941 gagggaagat aaagccatgg tgtctctcct ctttcttgtg tgtgagtcgt cccggagttg
     3001 ctggagatag agacgggatc ggcggcgacc attgctcccc tagctgtcca ctcgcgccgc
     3061 cgaccggcct cacccccctc tccgcaagag gcgtgccatg aggccctcac ccctggccgc
     3121 ctctccctct ccgggagagg gagggtgggg cggcgggaga gggggccggg ggctgagagc
     3181 cgtttttaca accccaccgg cgttcccacc ggtggcgccg gcggcggtgc cggcggggtg
     3241 gccgtgggag acggctccac ggtgccgacg ctgacgtcga tctccgtttc gtcctccccc
     3301 tcactggccg gggtgtccgc cgcggccgcc tccggcggcg ccggctgagc ggtgccggcg
     3361 aggggcggct cgggtggcgc ggggcgctgg ctgctgggcc ggatatagga gccgtcctcc
     3421 tccccctccc cgacggcgac accctcctca gcctccgtgt cgatctcggc ggggtcgacc
     3481 cccaccggct ccagcgggtt cacccgcacc gtgcgcggcg gcagcgccgc cggctggttg
     3541 gtgatcaccg gccgggggcc ttcctgatcg atgtaggtgt cgagcaggta gggatcgacg
     3601 ggctcgccgg cgtagggatc ctcttcagag tactggccgt agggatccgg atccgtggcg
     3661 tacgggtcgt agtacccgcc ggcgtccgcg tagatgcggc cgccgctcgg atggccgtgc
     3721 cggtggcagg gctcggtggg gaccttccag tcggggaaga cgtcggtcac cacgtacggg
     3781 caggcgtcgg tcgcgagctg gccggtggtc ggatcgatgg cgagttggac gatcccctgg
     3841 ggccgcgcga agtccttgta gccgccggcc ggccgcaccg ccatggtgaa gcggctccac
     3901 accggcagcg ccgccgtcga gcccgagaag cgggtgcggc ggttgtcgtc atagcccacc
     3961 catacgaccg tcacccggtc cggcgagtag ccggcgaacc agttgtcccg ccggtcgttg
     4021 gtggtgccgg tcttgccggc cagccggccc tgcacacccc agccgcgggc ggcggcggcg
     4081 gtgccgcggt cgagcacccc ctggagcccg gaggtcacga ggtaggccac ctgcggcgag
     4141 agcacccgct gcggcgactc gagctcctcc ccatccacct cctggccctc gcggtcgagc
     4201 accagcttca ggccgtggac ccgggggcgc agcccgtcgt tggcgaacac cgagtagacc
     4261 tccgcgagct cgcgggggga gacctcgaag gtgccgagcg ccagggccgg aaccgtatcc
     4321 atctcccctt cgatccccat gtcccgcgac aggtcgacca cccggtgcag gcccacctgc
     4381 agggccaggc ggatggtggg gatgttgagg ctgcgctcca gcgccgtgcg cgccgtcacc
     4441 gatccctgga agccgcggtc gtagttctgc ggccgccagg gcttgttgtc cactttcacg
     4501 gtgatggggg agtcgcgcag cagggtcacc ggcgtcgcca ccgattccgc gaaggccgcg
     4561 gcgtacacca ccggcttgaa cgcgctcccg gcctgccggc gcgcggtgga gacgcggtcg
     4621 aactggctgt cggcgtagtc ccggccgccg acgtaggcga ggatcgcgcc gtcgcgggga
     4681 tcgacggaga cgagcgcccc ctggagggga tccttaccgt tcttgccgcc gcgcgggtcg
     4741 aggctggcga ggccgcgttc cagcgcccgc tgcgcctcct tctggtcacg ccagcggagg
     4801 gaagagaaga gcaggtagcc ctcgccgcct atgtcctcga tgtcgaagcg ctcggtggcc
     4861 tcctgtttgg cgaattcggc gaagtagggg gcgagcggcc gggtaaccag cttctgcggc
     4921 cgggccacga ccggcgtggc ggccgcccgc tgccgctgct ccggggtgat ccagtccaac
     4981 tcgtccatac gctgcagcac ccagtcgcgg cgctcgcggg cctcctcggg atgctgcgtg
     5041 ggcaggtagt tggccggcgc ctggatcatg ccggccaggg tcgcggcttc ggagagcgtc
     5101 agctcccgcg gctccttgcc gtagtaggcg taggaggcgg cccccaggcc gatcaggttg
     5161 accgagctgc tggcacccca gtagatctcg ttgaggtagg cctcgaggat cgctttcttg
     5221 ccgtgccgga tctccagcat catggcgatc agcgcctcct tggccttgcg cgacagcgtc
     5281 cgctcgctcg acaggtagac attcttgacg agctgctggg tgatggtgct gccgccctgc
     5341 acctcgccct cgccgcgcag gttcacccag agggcgcggg cgacgccggt gggggagacg
     5401 cccgggtggg tgtagaagcc gtcgtcctcg gccgccagga tcgcctggat cacgtgctcc
     5461 ggcacctggt ccagcgtcac cggccggcgc tcctggacct cgtcgtcgta gtaggaggcg
     5521 aggagcggcg gcggcagcgc cgcctcgtcc actacccggc ccgcctggcg gatctcgccg
     5581 atgcgccggc cgcggaaggt cacctccagc aggccgcccg gagcgggccc ctccgtcgtg
     5641 ggggaggagc gcaggtcgac ggccaggcga tcctcctggc gccggaaggt gcccggcggc
     5701 agcggccgcc cggcgtcacc ctcgcctccc tcggccgcgc tctcgccctc gtccttgcct
     5761 tcggcctcgt cagcctcacc cttctcgctc tcctcctcgg cttcgcgata gccctggctc
     5821 tccagctccg cgacgatatc gtcgatcccg atcccggcgc ccggagcgag ccgggtggct
     5881 tcaccataga ggcgcgacgg ctgcttgaac ggtgcctggg ggaaccgtcg ggcgaggtcc
     5941 cagagataga cggcgccggc gaggcccaga accagcacca gcccgaaagt ggtgaggact
     6001 ccgatcacca cccggcggcg ggcgggcggc atgcccgcca ggtctcgacg gaagctgttg
     6061 taggtctctc gcatcatgtc tgctccgccg gctccgaagc cctgccgcga ggttcgcggc
     6121 ggcgcggggg ccgctctacc ggtaggacat ggcaatcgcc atgccacggc cggcatagcc
     6181 gttgcagttc tgccctttcc gtggggggcg ccgccggaac acctttccgg ccgcggaaga
     6241 agtagtaagg agacgtgatg agcgacgtgg aagcgaaatc cgccctcaag gggctggagt
     6301 taaggcagct ctatgcccgg ctcgacctcg cgggatggac gctgatcagc gagcaccacc
     6361 tcgagaagga gtaccggttc aacgatttcc gggaggctct cgccttcgtc aaccgcctcg
     6421 gcgaggtggc ggaggaggag gggcaccacc cggacatctt cctcacctgg ggcaaggtga
     6481 agctgacgct ctggacccac tcggtagggg ggctgtcgga gaaggacttc attctggccg
     6541 agagagcgga ccggctcctc tgaggtagag gcggggggcg acggagcgcc ccccggggga
     6601 ccctctacga cgatggcatg cggcaactgt cgccgccggc ggcgggcacg tagctctcgc
     6661 ggacgtagtc catcaccatg cggtcggcgt tgtagcgcca ggccatggtg cggatggcgc
     6721 gcttgacgcg ggcgatccag ccgtacggca ggccgtcggc gtcgcgctgg taatagaggg
     6781 ggaccacctg ccgctccagc gtctcgaaga gcgccagggc gtcccgctcg tcctgcacgt
     6841 cggggttggc gtggaccgcc tcgccgccca ccgcgaagcc gttggcgccg tcgtacccct
     6901 cggcccacca gccgtcgagg atggagacgt tgagcacgcc gttgaggatg cacttctggc
     6961 cgctggtgcc gcaggcctcg agcggccggc gcggcgtatt gagccaggcg tccaccccct
     7021 ggacgagctg ccgggcgacg tgcatcgagt agttctcgat gaagacgatg cggccggcaa
     7081 agcgcgggtc gtgctcgagg ttggcgacgc gctgcgccag cgccttgcca ccggtgtcgc
     7141 gggggtgggc cttgcccgcg tagacgatct gcagcggccg ctccgggtcg ttgaccaggc
     7201 ggtcgagccg gtcgaggtcc tggaacagca ggtcggcgcg cttgtaggtg gcgaagcgcc
     7261 gggcgaagcc cagggtgagg gcgtcggggg ccagcggttc gttctcgggc tcgggcatgc
     7321 cgagccgctc ccgccgctcg gccaggcgcc ggcgggcgaa ggtgaagagc cgcgccttga
     7381 gcacctggtg cacctcccag agctcggcgt ccggtacctt ggcgatgcgc gaccagagct
     7441 ccggccggga gagcgactcc agccagctcg gcccgatgta gcgcgccatc aggtcgtgca
     7501 tgtcggcggc gagccaggtg cgcacgtgca cgccgttggt gatgtggccg atcggcacct
     7561 cctcctcggt gcggccgggg tagagggagt gccacatctt gcgcgacacc cggccgtgca
     7621 gggccgagac
//
LOCUS       DASPJI010000635         6094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_500102, whole genome shotgun sequence.
ACCESSION   DASPJI010000635 DASPJI010000000
VERSION     DASPJI010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500102"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2188)
                     /locus_tag="VEL74_17775"
     CDS             complement(<1..2188)
                     /locus_tag="VEL74_17775"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG64432.1"
                     /translation="MSVATKSGRFFKASRDRLELLRELRREEGLETAAAPMIPRRQDP
                     SSHPLSFSQERLWFLDRLDPENPAYNIPVSLRLRGPLDAAALARALGEVIRRHEVLRA
                     TFHEAGGRPLQHLVPATVAMVFLPVIDLRSLPPGRRDEEARRAAGGAASRPFRLDEGP
                     LLRARLLRLGDEEHALGLEVHHIVADGWSLGVLVRELTALYAGETLPELPIQYADYAA
                     WQRERLTGESLAELTAWWRERLAGSPPSLDLPTDRPRPAVQTFHGGHAACSLPAALTD
                     RLRSLAGEAGASLFMVLLAAVQLLLARWSGQDDVPVGSPIAGRTRREVEGLIGLFLNH
                     LVLRSDLSGDPAFRELLDRVREVAVGAYAHQDLPFEKLLAEIEPQRDLSRTPLFQVFF
                     NLLSFPAPDVRLSGLTIEGLEAPPEAQSKFDMTFYLVEQVGGVRIDLVYNADLFDAAR
                     MDEALAQYRLLLEQAVAAPDARIGSYSLVTTAARTLLPDPTEELDACWPGSVAEALAR
                     HARATPDRPAVLWEGGELSYSQLDAEVERVAAELRAAGIGRGEVVAIRGHRSAPLVAA
                     VLGVMRAGAAFLILDPAYPEPRIGQMLELAKPAGLIDLEGDGASPPGGRVVGSGGGQE
                     GGCLSGGGGDDLAYLAFTSGSTGTPKGVLGLHRSLTHFYPWMSQAFQLGPDDRFSLLS
                     GLAHDPLHRDVFTPLWLGAAVVIPDPERMTEPGWLAGWMARTGVTVA"
     gene            complement(2185..>6094)
                     /locus_tag="VEL74_17780"
     CDS             complement(2185..>6094)
                     /locus_tag="VEL74_17780"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64433.1"
                     /translation="RPDLTAERFVPDPRAPGARRYRTGDLARRRPAGALEFLGRRDHQ
                     VKVRGLRVEPEEVEAALLACPGVNEAAVVARDGALVAYVVGGVGDIRPTLAARLPAFL
                     VPSAFVPVDTLPRTASGKVDRRALARREVQAAALAETGAFEPPRTPVEEQVAAIWSEV
                     LGRPRIGAHDDFFALGGHSLLALQVVSRLRAAFGLEVPLRRLFEAPTPAALAREVETA
                     LLAGPHPLPPPLVRVPRDREIPLSFSQESLWLLHQLDPASPAYNVPAAVRVSGRLDPA
                     ALERTLGEIVRRHEALRTTFPGGRPVQVIHPLRPMPLPSVDLAGLPPALREAEARRLA
                     GNEALRPFDLARGPLLRAALARLGDEEHLFLLTLHHAVSDEWSMAVLVREVGAIYPAL
                     AAGLPSPLPELPIQVADHAVWQREWLRGEVLEERLAWWRERLSGAPPMIELPLDRPRA
                     AARSFRGGRVPLEVPGAAAQGLRQVGRERSASLFMVLLAGFQAWLGRHGAGLDLVIGA
                     PVANRSHPGTEGLIGFLVNTLPLRGSLAGDPGFGELVERARETCLGAFAHQDLPFERL
                     VRELRPARSLAYAPLFQVMLALHNVPTPALDLAGLSFRPLEMDPGTAKFDLVLELSEG
                     LGETPSILGSLEYAADLFDRTTAERLAGHLRVLLEAAAADPELPVAALPVLTDVETRQ
                     IANWSAARAAFPAACLHRLFEARAAERPEAVAVGPMTYADLDRQANRLARRLRTLGVG
                     VETRVALRHERTPGLIAGMLGILKAGGAYVPIDPAWPAERQKLLLEDAGAALLVDEEL
                     LTASQDESAEPLEVPVTQYNLAYVIYTSGSTGRPKGTLVTHGHVSRLLAATQPWFGFG
                     PDDVWTLFHSAAFDFSVWEVWGALAWGGRLVVVPTTTARSAEDLWRLLSMEGVTVLNQ
                     TPSAFRPLSRLPVPPSLRWVIFGGEALDVGTVASWLGGPRGEGPRLVNMYGITETTVH
                     VTYRPLSEEDLETPWRSPIGVPIPDLSVHVVDRRGAAVPAGVPGELCVGGAGVARGYV
                     GRPDLTAERFVPDPFSGLPGARLYRSGDLARRLPGGALEFLGRMDRQVKLRGFRIEPA
                     EIEAALTAIQGVREAAVLLRDDLPGGPGLVAYVVSGLDNRIDAGDLLAALRSRLPEHM
                     VPGRFVFLPELPLTPNGKLDRQALPAPEGGRPGLARPYVAPLTPLERVLVGAWEEALG
                     VDRVGVDDGFFDLGGHSLLATQVISWVQETFEVEVPLRALFERPTVAGLATALTSGAS
                     DAATRQRVERTAELLLDVSELSEEEVAGRVHGATSSVGPA"
BASE COUNT          938 a         2196 c         2260 g          700 t
ORIGIN      
        1 gggcgacggt gacgccggtg cgcgccatcc agccggcgag ccagccgggc tcggtcatcc
       61 gctccggatc ggggatcacc accgcggcgc cgagccagag gggggtgaag acgtcgcggt
      121 gcagcgggtc gtgcgccagg ccggagagca ggctgaatcg gtcgtcggga ccgagctgga
      181 aggcttggct catccaggga tagaaatggg tgagcgagcg gtgaagcccg aggacgccct
      241 tgggggtgcc ggtggagccg gaggtgaagg cgagataggc gaggtcgtcg cctcccccac
      301 cggaaagaca acccccctcc tgacctcccc cactccctac cacccgtcct ccaggggggg
      361 aggcaccatc gccctcgaga tcgatcaagc cagccggctt cgcgagctcg agcatctggc
      421 cgatgcgcgg ctcggggtag gctgggtcga ggatcaggaa cgcggcgccg gcgcgcatca
      481 cgccgaggac cgcggcgacg agcggcgcgc tgcggtgccc ccggatcgcc accacttcgc
      541 cgcggccgat accggcggcc cgcagctcgg cggccacgcg ctccacctcc gcgtcgagct
      601 ggctatagga tagctcgccg ccttcccaga gcacggccgg ccggtccggg gtggcgcggg
      661 cgtgccgggc cagggcctcg gcgaccgagc cgggccagca cgcgtcgagc tcctccgtgg
      721 ggtccggcag gagggtgcgg gcggcggtgg tcaccagcga gtagctgccg atccgggcgt
      781 cgggtgcggc cacggcctgc tccagaagca gacgatactg ggcgagcgcc tcgtccatcc
      841 gcgccgcgtc gaagaggtcg gcgttgtaga ccaggtcgat ccgcactccc ccgacctgct
      901 ccacgaggta gaaggtcatg tcgaacttcg actgcgcctc gggaggagcc tcgagccctt
      961 caatcgtcag gccggagagg cgaacgtcgg gagccgggaa gctcagcagg ttgaagaaga
     1021 cctggaagag aggtgtgcgg gagaggtcgc gctgcggctc gatctcggcc aggagcttct
     1081 cgaaggggag gtcctggtgg gcgtaggcgc cgacggccac ctcgcgcacc cggtcgagca
     1141 gctcgcggaa cgccggatca cccgacaggt cggagcgcag cacgaggtga ttcaggaaaa
     1201 gcccgatcag cccctcaacc tcgcggcggg tgcggccggc gatcggagag ccgaccggca
     1261 cgtcgtcctg gccgctccag cgcgccagga ggagctggac ggcggccagc agcaccatga
     1321 agaggctcgc tccggcctct ccggccagcg accgcaggcg gtcggtgagc gcggcgggga
     1381 gggagcaggc ggcgtgcccg ccgtgaaagg tctgcaccgc cggccgtggc cggtcggtcg
     1441 gcaggtcgag ggacggcggc gagcccgcca gccgctcccg ccaccaggcg gtcagctccg
     1501 ccagcgactc gccggtgagc cgctcgcgtt gccaggcggc gtagtcggcg tactgaatgg
     1561 gcagctccgg gagggtctcc ccggcataga gcgccgtcag ctcgcgcacc aggacgccca
     1621 gggaccagcc gtcggcgacg atgtggtgca cttcgaggcc tagggcgtgc tcttcgtctc
     1681 cgagccgtag cagccgggcg cggaggagcg gcccctcgtc cagccggaac ggccgcgaag
     1741 ccgcgccgcc ggcggcgcgg cgggcctctt cgtcccgccg gccgggagga agggaccgca
     1801 ggtcgatcac cggcaggaac accatggcca ccgtggccgg caccaagtgc tgaagcggtc
     1861 tgccgccggc ctcgtggaac gtcgcccgga ggacctcgtg ccggcggatg acctcgccca
     1921 acgcccgggc cagggctgcc gcgtccagcg ggccgcgcag ccggagcgag accgggatgt
     1981 tgtaggccgg gttctcgggg tcgagccggt cgagaaacca gaggcgctcc tgggagaagg
     2041 agagagggtg ggatgacgga tcctggcgcc gtgggatcat cggcgccgcc gcggtctcca
     2101 gcccttcctc ccggcgcagc tcgcgcagca gctccagccg gtcgcgggag gctttgaaga
     2161 aacggccgga cttcgtcgcc acgctcatgc cggacccacc gagctggtgg cgccgtgcac
     2221 acggccggcg acctcctcct cggacagctc gctgacgtcg aggagcagct cggccgtccg
     2281 ctccaccctc tgccgcgtgg cggcgtcgga cgcgccggag gtcagcgccg tcgccagccc
     2341 ggccacggtg ggccgctcga acagcgcgcg cagcggcacc tcgacctcga aggtctcctg
     2401 gacccaggag atcacctgag tggcgaggag agagtgcccg ccgagatcga agaagccgtc
     2461 gtcgactccc acacggtcga ccccgagggc ctcctcccag gctccgacga ggacccgctc
     2521 cagcggcgtg agcggtgcca cgtagggacg ggccagcccg ggccggcccc cctccggcgc
     2581 cggcagggcc tgccggtcga gcttgccgtt cggggtgagc ggcagctcgg gcaggaagac
     2641 gaagcggccg gggaccatgt gctccggcag ccgggagcgc agagcggcga ggaggtcgcc
     2701 cgcatcgatc cggttatcga gaccgctcac cacataggcc accagcccgg gcccgccggg
     2761 caggtcgtcg cgcaggagca ccgcggcctc ccgcactccc tggatagcgg tcagggccgc
     2821 ctcgatctcg gccggctcga tacggaaccc ccgcagcttc acctgccggt ccatgcggcc
     2881 caggaactcc agcgcccctc cagggagccg gcgcgccagg tcgccgctac ggtagagccg
     2941 ggctcccggc aaaccggaga atggatcggg gacgaagcgc tcggcggtca ggtccggccg
     3001 gcccacgtag ccccgggcca cgccggctcc gcccacacac agctctcccg gcacaccggc
     3061 cggcaccgcc gcgccacgcc ggtcgacgac gtgcacggag aggtccggga tcggtactcc
     3121 gatcgggctg cgccaggggg tctccaggtc ttcctcggag agcggccggt aggtcacatg
     3181 gaccgtggtc tcggtgatgc cgtacatgtt gaccagccgc ggtccctcgc cacgcgggcc
     3241 gccgagccaa ctggcgacag tcccgacatc cagggcctct ccaccgaaga tcacccaacg
     3301 cagcgacggc ggcaccggca gccgcgagag cggccggaag gccgagggag tctgattgag
     3361 gacggtcacg ccttccatac tgagaaggcg ccacaggtct tcggcggagc gggccgtcgt
     3421 cgtggggacg accaccagcc ggccacccca ggccagcgcg ccccagacct cccagactga
     3481 aaagtcgaaa gccgccgagt ggaaaagcgt ccagacgtcg tccggcccga acccgaacca
     3541 cggctgcgtg gccgcgagca gacgcgagac gtggccatgg gtcaccaggg tccccttggg
     3601 ccggccggtg gaccccgagg tgtagatgac gtaggccagg ttgtactgcg tgacgggcac
     3661 ctccagcggc tcggcgctct cgtcctgaga ggcggtgagc agttcctcgt cgaccaggag
     3721 cgcagccccg gcgtcttcca ggaggagctt ctggcgctcg gcaggccacg ccggatcgat
     3781 cggcacgtag gctccgccgg ccttgaggat gccgagcatg ccggcgatca ggcccggcgt
     3841 gcgctcgtgt cgcaacgcaa cccgtgtctc cacgcccacc cccagggtac ggaggcggcg
     3901 tgccagccga ttcgcctgac gatcgaggtc ggcataggtc atgggtccca cggcgaccgc
     3961 ctccggccgc tcggcggccc gagcctcgaa caggcggtgc aggcaggcgg cggggaacgc
     4021 cgcccgtgcc gcgctccagt tcgcgatctg gcgggtctcg acgtcggtca gaaccggcag
     4081 cgcggcaacc ggcagctccg ggtctgcggc ggcggcttcc agcagcaccc gcagatggcc
     4141 agccaggcgc tcggcggtcg tccggtcgaa caggtccgcg gcgtactcca gcgagccaag
     4201 gatcgacggc gtctccccca gcccttcgct aagctccagc accaggtcga acttggccgt
     4261 gccggggtcc atctccagcg gccggaacga caggcccgca aggtccaggg ccggtgtcgg
     4321 gacgttgtgc agagcgagca tcacctgaaa tagcggcgca taggcgaggc tgcgcgccgg
     4381 ccgtagctcc cggaccaacc gctcaaaagg caggtcctgg tgggcgaacg cgcccaggca
     4441 cgtctcgcgc gcccgctcca ccaactcgcc gaagccgggg tccccggcga ggctgccacg
     4501 caacggcagc gtgttgacca ggaaaccgat cagcccctcg gtgccggggt gggagcggtt
     4561 ggccaccggc gcaccgatca ccaggtccaa tccggcgccg tgccggccga gccaggcctg
     4621 gaagccggcg agcaggacca tgaacaggct tgcggagcgc tcgcgcccaa cctgccgcag
     4681 gccttgtgcc gcggcgcctg ggacctccag cggcacccgg ccgccacgga agctccgcgc
     4741 cgcggcgcgc ggccggtcca ggggcagctc gatcatcggc ggcgctccgg acagccgctc
     4801 ccgccaccag gcgagccgct cctccaggac ctcgccgcgc agccactcgc gctgccagac
     4861 ggcatggtcc gccacctgga tgggcagctc gggcagaggc gagggaagac ccgcggcgag
     4921 cgccgggtag atcgccccca cctcgcgcac cagcaccgcc atcgaccact cgtcggagac
     4981 ggcgtggtgc agggtaagga ggaaaagatg ctcctcgtcc ccgagccgcg ccagggcggc
     5041 gcgcagcagc ggtccgcggg cgagatcgaa gggacggagg gcctcgttgc cggccagacg
     5101 ccgggcctcg gcctcccgca aggcgggtgg caagcccgcc aggtcgaccg acgggagggg
     5161 catcggccgc agcggatgga tcacctgcac cggccggccc ccagggaagg tggtgcgcag
     5221 cgcctcgtgc cggcggacga tctcgcccag ggtccgctcc agcgcggcag ggtccagccg
     5281 gccggaaacc cgtacggcgg cgggcacgtt gtacgccggg ctcgccgggt cgagctggtg
     5341 gagcagccag aggctctcct gggagaagga gagggggatc tcgcggtcgc gcggcacgcg
     5401 caccagcggc ggcggcagcg gatgggggcc ggccaggagg gcggtctcga cctcgcgcgc
     5461 cagggcggcc ggggtcggcg cctcgaacag ccgccgcagc ggcacctcga gcccgaaggc
     5521 cgcgcgtagg cggctcacca cctgcagtgc cagcagcgag tgcccgccga gggcgaagaa
     5581 gtcgtcgtga gcgccgatcc gcggacggcc caggacctcg ctccagatgg ccgcgacctg
     5641 ctcctcgacc ggggtgcgcg gcggctcgaa ggcccccgtc tccgcaaggg ccgcggcctg
     5701 cacctcacgg cgcgccagcg cccggcggtc caccttgccg ctggcggtcc gcggcagcgt
     5761 gtcgaccggt acaaaggccg aagggaccag gaacgccggc aggcgggcgg ccagggtcgg
     5821 ccggatgtcg ccgacgccgc caaccacgta cgcgacaagt gcgccgtccc gcgccacgac
     5881 cgcggcttcg ttcaccccag ggcaggccag gagggccgcc tccacctctt ctggctcgac
     5941 gcgcaggccg cggactttga cctggtggtc ccgccggccg aggaactcca gagcgccggc
     6001 cggccgccgg cgcgccaggt cgccggtgcg gtacctccgg gctccggggg ctcgcggatc
     6061 ggggacgaag cgctcggccg tgaggtccgg ccgg
//
LOCUS       DASPJI010000636        14884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_338345, whole genome shotgun sequence.
ACCESSION   DASPJI010000636 DASPJI010000000
VERSION     DASPJI010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14884)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14884
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338345"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VEL74_17785"
     CDS             complement(<1..725)
                     /locus_tag="VEL74_17785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004579011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HYG64434.1"
                     /translation="MKKVLSLVLLAALAGAAHAQNTTQQPAQGAARPKPESVDDRANY
                     IIGLNLGKSMKQQDVKANVDLILQGLRDGLAGGQALLTDEEMQAAMTAFQQGLMARQQ
                     EKMAAAATQNKTEGESFLAANKAKEGVTTTASGLQYQVIKPGSGEPPKPGDRVTVHYT
                     GTLIDGTVFDSSRERGQPATFGVTQVIPGWVEALQLMKPGAQYKLFIPSNLAYGEQGA
                     GGGEIGPNATLIFDVELVSVEKGG"
     gene            complement(795..2474)
                     /locus_tag="VEL74_17790"
     CDS             complement(795..2474)
                     /locus_tag="VEL74_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HYG64435.1"
                     /translation="MKFKADESEVKLRGGYYTRPEIASFLLRWALGIAPRHLLEPSCG
                     DGVFLARLATLLPADAPRPLARLPTVTAFEIDPAEAGKAQAAAERIPGAEVHVRDFLS
                     WSLERLGGPPVFDAAVGNPPFIRYQYLSPESQEIAERIFRSLGLAFTRHVNAWVPFVA
                     AALALLRPGGRLAMVLPAELLHVLHAQSLRTFLAGQCSRVLLFDPQELWFDGVLQGAV
                     LLLAEKRELGTSEEPGRIAIIPTRAESFLDQDPEDLFRTADWAPLGGPVLSGKWMPAL
                     LSRREREVLTAAAGLPGVRRFAEVADVDIGIVTGANKFFLVSDGEVERHGLGAWAFPM
                     FGRSEHVPGVIYDEETHVGNRRAGLPSNFLWFTREELADLPPAVRGYLERGAAEGLPE
                     RYKCRIRQPWYSVPSVYATSVGMLKRCHHFPRLISNRLGAFTTDTAYRVRAKETEPED
                     LVFSFVNSLTALCAELEGRHYGGGVLELVPSEIEKLLLPLPLEEPSPGGRLERLDQEI
                     RTGTPAEELLRRQDDDLLAPVGLSSEEAGELRAAWSRLRSRRQRTDDDSRD"
     gene            complement(2562..4376)
                     /gene="aceK"
                     /locus_tag="VEL74_17795"
     CDS             complement(2562..4376)
                     /gene="aceK"
                     /locus_tag="VEL74_17795"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HYG64436.1"
                     /translation="MDLQSTNLAASRAADIIHWAFDEHQLRLRAITQRVRQRFERCDW
                     LGIRQDTVERMDLHPRAIHRTCDALREPLGDRFADRETWTALKEAYTREIIGRNDFEL
                     AQTFFNSLTRLVFPRTGVDPAIDYVADDFPLPYRGWEMASARMYAVRRIDAAVIRKVL
                     EDAGFRTSFRDLEGDAALAAERVQRGLREAFGSEDIQALDVLRPVFIRNKGAYVIGRA
                     RRGEEVRPVVLAMTNGRSGIEVDAVLHTEDETSVVFSFARWYFHADVESPREVIGFLR
                     SILPRKRISELYISLGYTKHGKTEFYRDLMHHIARSDDLFVPARGRRGLVMDVFTLPS
                     YPFVFKVIKDRFPPSKNTTREKVMEKYREVLAQDRVGRLVDFQEFEHLSFPRARFSEE
                     LLSEILQSSSQDVGIEGDTVVVRHAYVERRVTPLDVFLAEAAEAGPEREGAALSALSA
                     LSAVDDWGRCLRDLAAANIFPGDVLLKNFGVTRHGRVVFYDYDELASLTSCRFRPFPA
                     ARHHEDEMAAEPWFSVGEHDVFPEELRTFLGLEGALRDAFLARHGELFDPGFWQGMQE
                     RNREGEVIAFYPYGEARRLRPAPATSGGAPARSDSAAA"
     gene            complement(4431..6113)
                     /locus_tag="VEL74_17800"
     CDS             complement(4431..6113)
                     /locus_tag="VEL74_17800"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HYG64437.1"
                     /translation="MLPRKPAEAERDHSDEPVRQGPRRISDSGPRETRPERPVTQRTL
                     LGWVLAAAVLLLIAVIAQHFDEYLRRTLEAKINQRLHGYTVTLAGAHLNPFGLALTLE
                     GAVIRQQAHPEPAVADIPRLTASVQWRELLRLHLVANAVFEQPRIHINLPQLRQEDRD
                     ELNVEDRGWQDALQSIYPLKFNLVQVRDGAITYVDHDPERPLEITRWNLSAENIRNIR
                     SDEGVYPSPVRTDGVLFGTGRGVIEGHADFLSKPHPGFHVVYELEKVPLERLDVLGSR
                     ANLDVDGGILGSRGRFEYSPKHREARIADVTIQGLKLDYTHSAATAGAEKRRAAEVSE
                     AAQDDQPPMPVHIERLELADSVLGLVNRNADRPYRVFVDGADLTVTNVSSGFREGPAT
                     AKLTGRFMGSGAASATATFREEGSGPDFDLNVAIEGASLPSMNELLRAYGKLDVAEGT
                     FSVYSEIKVKNGRIDGYVKPLIKDVKVYDPEQDKEKPVLKKIYEKVAGGLANILDNRP
                     RDEVATVADISGPISDPDTSTWEVIVRLVSNAFVKAILPGFDREFDAAREGR"
     gene            6272..6499
                     /locus_tag="VEL74_17805"
     CDS             6272..6499
                     /locus_tag="VEL74_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019004944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HYG64438.1"
                     /translation="MDGTRRQDIRPGLEVEIVLKQDQATGKRTRGIVREILTPSPTHP
                     HGIKVRLTSGAVGRVKAILTKGKETPGPATT"
     gene            6532..7107
                     /locus_tag="VEL74_17810"
     CDS             6532..7107
                     /locus_tag="VEL74_17810"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HYG64439.1"
                     /translation="MTASEQPAQPILPLLEEGAGEITPPLPSIADGVEHLLDPRHVTL
                     SRIVRWLGAGAVSLGSLVPLGSIVFFASIPGWVTGILPLLWVVGTLGLAWSAHRWPVI
                     EHRHASYKVDDNGIQIRQGVVWRKEINVPRSRVQHTDVSQGPLERRFGLGTLVIYTAG
                     TDHAKIELHGLDHATALAIRDHLVHGEGDAV"
     gene            7097..8701
                     /locus_tag="VEL74_17815"
     CDS             7097..8701
                     /locus_tag="VEL74_17815"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HYG64440.1"
                     /translation="MPSEPADSAVADRRLHPLSIFFGFAAQIPKLVLPGVFVLFSARS
                     SQWAEWQLWAMLLLIPYTLVAVGRYITLRYRYEPHEIVIRTGLLFRNERHIPYSRIQN
                     LDAVQNVLHRLAGVVEVRVETGGGQKAEATMRVLPVAAYEEMRRRVFAGKAAEAVAEG
                     EAAESVPVPASTPERTLLRMPLHDLLICGFIENRGIVIIGAGFGLLWEAGLMDRLMGR
                     WLRVNGPGGGLPEWFGGDAPGGGLSAAVVKAFLGQGELPLTRIALALTAFAGILLLIR
                     LLSMIWALLRLYGFRIVRAGEDLRTEYGLLTRVVATIPLRRIQTLTIREGPLHQLFRR
                     AAVRVETAGGGGGGDGEEESTAKQREWLAPLIHRDALPGFLREVLPEADLSTVEWRPV
                     EPRAFRRHLKKSLIADVLIALPFVLMLKFWTLALLAVFIAWSVVAAHLYVRHLGWAVI
                     DGAVLFRSGWLWRRVSVARFAKIQAVTIHESPWDRRAAMARVRVDTAGARGEAQRVDI
                     PYLARDTARALYELLSGQAAQTAFRW"
     gene            8889..10136
                     /locus_tag="VEL74_17820"
     CDS             8889..10136
                     /locus_tag="VEL74_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64441.1"
                     /translation="MLLLYDSKCSICRSLAYKIHFLTESNVEIESLHSPNAETVLSRF
                     YPQGWQHDFYVVHNGTCRKGLRALPSLMRSVGLKRMASLVSEYGSYKLAPKACGATDA
                     NGNGDGVVAPKRNFLKLAAMTPVAYGLSHLRLENPFEERDRPKLDLSVHVAEVEMTGP
                     GEFRVKAYRCDNCGRVPAPDKGLPAGASKRLLDQVMLAEDSIDGFAQKGLGTANFKVQ
                     RIEYEREMPENGSLSREKRLVHAALLDHPEYNLSVNIGQGRTTMLAGMARHDLPLPML
                     DYLIFRPDTEEDVVTHLAAYRSGLLELAKLHGRQGRQALARVYREMAQGFMVMSRRFD
                     AAVPERLLPAKNEIVVTSVPELLRFVQKPPDLQHSEKACDCSCSCNVCCGCGCSFGVC
                     IEFVSPCGCDCCLACGCGCGCCL"
     gene            complement(10221..10904)
                     /locus_tag="VEL74_17825"
     CDS             complement(10221..10904)
                     /locus_tag="VEL74_17825"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HYG64442.1"
                     /translation="MKTLIVAAVLSLASLLLMAAPPAWSDQAGARAQLEKEGIPFTPE
                     AFLEKVAEGDVARVGLFLDAGIDPSVRNDSGRTALWVATERRQLAVLQSLLAFGVKPD
                     AKNAPMGEGGKSLVFEAVDSGDAAFVTALAEAGADVKTANDYEVPPLSEAARVGNLAM
                     CEALLKAGADPNAAPAGFPLIFGPVNENHPEVVRLLLKSGAKLGEHKQTLVEAATHPE
                     IRAMLEAAE"
     gene            complement(11511..11864)
                     /locus_tag="VEL74_17830"
     CDS             complement(11511..11864)
                     /locus_tag="VEL74_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64443.1"
                     /translation="MPPKKNETLDRWRRLLPPLEAHAGELPHLESLRGKLKGTLERAE
                     EIIQRQAALEAERQELTREFGGLRDQADRTAALLRTGISEHYGPDSERLAAFGMQPFR
                     GRKRKAAPENGSGEA"
     gene            12092..12430
                     /locus_tag="VEL74_17835"
     CDS             12092..12430
                     /locus_tag="VEL74_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64444.1"
                     /translation="MIDSSNTHERYVTDGAGNRIGVILTLDEYSQLMEAAEELEAIRA
                     FDDAKASGDEAIPFEQAGHEVQPGERDPLGNLVGLLRHRANSEPVSVEEMGEAIRARA
                     AEKYGDRKGG"
     gene            12468..13901
                     /locus_tag="VEL74_17840"
     CDS             12468..13901
                     /locus_tag="VEL74_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64445.1"
                     /translation="MELSSRLREIEHEIDSKIRGLEVWQAPRAEVLEHLVYTYRDLIE
                     EAFMRLLHARTFGLPPDEAFYLENRGRNGMLWALKWASEYCPESGKAHLRSPDDLMEL
                     VRLGGVYEVFVDALKYAGRGLVTIHVEESSKIITCYEGSPATAFDASIVDRERIVSPQ
                     MPHVSLTEDGDRITTRWTAGDYRKITKELAQYGASEENAIVVDPEFALAAGMSNLSVP
                     QPTVVWVNRPADAPGCHVFDDLVLPVVMDSKVKWQLVSLLENPVIKVGERYCALSSDL
                     RAISEIDDYMLRLAARVDQRQYSLASVRREERMLAICRRALEQASPPWAVEEQVRYKK
                     PTQEADILAKRGADKIAIELKSTLRPETPWEVFKRNQDLVKGVKQVKRLIDRSVAERG
                     FLVTDGYRGDYVCWAEALANEVPIGNLYDLDIIAEDPSGALRRLKDLVGIPTADIRIP
                     EGLPDREENLFEWTLRLVDRKSPDHPG"
     gene            complement(13945..14442)
                     /locus_tag="VEL74_17845"
     CDS             complement(13945..14442)
                     /locus_tag="VEL74_17845"
                     /inference="COORDINATES: protein motif:HMM:NF037830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64446.1"
                     /translation="MKRPSGTKRPSLPEPIFFTDRDLGKIVPGALREGGLLVERYCDH
                     FQEANVPDEEWLSFIARRGWIGLSHDSNIRRDTVAVRATLENGGKLFIIRGSLVARDL
                     ADLFLGALKSVRSIIARHHDEAFIAVVRRATARGGLVVSEARVLKTASRVSEGQEESF
                     NPDED"
     gene            complement(14402..>14884)
                     /locus_tag="VEL74_17850"
     CDS             complement(14402..>14884)
                     /locus_tag="VEL74_17850"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HYG64447.1"
                     /translation="VLLALRKQYQVKMKEIRTALAYAQEQFGIERVLLSPELRATRGN
                     VFLQHLDSLVNVGRGGQGAMPEILSAYLERIEWDVAGAPIRMFPLTRPDQPHSPRLLA
                     INPRIAFGRPVVERKAIKTSIIAERFTAGESISELAEDYDLEVFEVEEAIRYEAPLAA
                     "
BASE COUNT         2414 a         4757 c         5162 g         2551 t
ORIGIN      
        1 ccgcccttct ccaccgagac gagctcgacg tcgaagatca gcgtcgcgtt cgggccgatc
       61 tcgccgccgc ccgcgccctg ctcgccgtag gccaggttgg acgggatgaa cagcttgtac
      121 tgggcgccgg gcttcatgag ctgcagcgcc tcgacccagc ccgggatcac ctgggtgacg
      181 ccgaaggtgg ccggctggcc gcgctcgcgg gagctgtcga agaccgtgcc gtcgatcagc
      241 gtgccggtgt agtggacggt cacccggtcg cccggcttcg gcggctcgcc gctgcccggc
      301 ttgatcacct ggtactggag gccgctggcg gtggtggtca ccccctcctt ggccttgttg
      361 gcggccagga aggactcccc ctcggtcttg ttctgggtgg ccgcggcagc catcttctcc
      421 tgctggcggg ccatgagacc ctgctggaag gcggtcatcg ccgcctgcat ctcctcgtcc
      481 gtgagcagcg cctggccgcc ggcgaggccg tcgcggaggc cctgcaggat caggtcgacg
      541 ttcgccttca cgtcctgctg cttcatcgac ttgccgaggt tcaggccgat gatgtagttg
      601 gcgcggtcgt ccaccgactc cggcttggga cgggcggcgc cctgcgccgg ctgctgcgtg
      661 gtgttttggg catgcgccgc tcccgccagg gcggcaagga ggacgaggga aagaaccttc
      721 ttcatggcat tctccaaagt tggggtcggc aacgccggcg gaacgacccg gcggctcgaa
      781 cggagaatct tacctcaatc gcgcgaatcg tcgtccgtgc gctgacgccg ggaacgcagg
      841 cgcgaccagg cggcccgcag ctcgccggcc tcctcggagg acaggcccac cggcgcgagg
      901 aggtcgtcgt cctggcggcg cagcagctct tcggccgggg tgccggtgcg gatctcctgg
      961 tcgaggcgct cgagccggcc gcccggtgag ggctcttcga gggggagggg gaggaggagc
     1021 ttctcgatct ccgacggcac cagctcgagg acgccgccgc cgtagtgcct cccctccagc
     1081 tcggcgcaga gggcggtcag gctgttgacg aacgagaaga ccaggtcctc cggctccgtc
     1141 tccttggcac gcacgcggta ggcggtgtcg gtggtgaagg cgccgagccg gttcgagatc
     1201 agccgcggga agtggtggca gcgcttgagc atccccaccg aggtggcgta gaccgagggg
     1261 acgctgtacc acggctggcg gatgcggcac ttgtagcgct cggggagccc ctcggcggcg
     1321 ccgcgctcga gatacccgcg caccgccggc ggcaggtccg ccagctcctc gcgggtgaac
     1381 cagaggaagt tgctgggcag gcccgcgcgc cggttcccga cgtgggtctc ctcgtcgtag
     1441 atcaccccgg gcacgtgctc gctgcggccg aacatgggga aggcccaggc gccgagcccg
     1501 tggcgctcca cctcgccgtc ggacactagg aagaacttgt tggcgccggt gacgatgccg
     1561 atgtcgacgt ccgccacctc agcgaagcgg cggacccccg gcaggccggc ggcggcggtg
     1621 aggacctccc gctcccgccg cgacagcagc gccggcatcc acttgcccga caggaccggc
     1681 ccccccagcg gcgcccagtc ggccgtccgg aagagatcct ccgggtcctg gtcgagaaag
     1741 gactcggcgc gggtggggat gatggcgatc ctccccggct cctccgaggt cccgagctcc
     1801 cgcttctccg ccagcagcag caccgccccc tggagcacgc cgtcgaacca gagctcctgc
     1861 gggtcgaaga gcagcacccg cgagcactgg ccggcgagaa aggtgcgcag cgactgcgca
     1921 tggagcacgt ggagcagctc ggccggcagc accatggcga gccgtccccc cggccgcagc
     1981 agcgccagcg ccgcggccac gaagggcacc cacgcgttga cgtggcgggt gaaggcgagg
     2041 ccgagcgacc ggaagatccg ctcggcgatc tcctgggact ccggggaaag atactggtag
     2101 cggatgaacg gggggttgcc caccgcggcg tcgaagaccg gcggcccgcc gaggcgctcc
     2161 agcgaccagg agaggaagtc gcggacgtgg acctcggctc cggggatccg ctccgccgcc
     2221 gcctgcgcct tgcccgcctc ggccggatcg atctcgaaag cggttaccgt cgggaggcgg
     2281 gccagggggc ggggggcgtc cgccggcagc agggtggcga ggcgggcgag gaacaccccg
     2341 tcgccgcagc tcggctcgag cagatggcgc ggcgcgatgc ccagcgccca gcgtagaagg
     2401 aatgacgcga tctccggccg ggtgtagtac ccgccccgga gcttgacctc ggattcgtcg
     2461 gccttgaact tcacggaggc ggattgtacc cggattcgag cccggtccgg cgccggctga
     2521 cggttcgctt tgtccggcgc aggagacgcg gccgggatcc tctaggcggc cgcggagtcg
     2581 cttcgggcgg gcgcgcctcc ggaggttgcc ggagccgggc gcaggcggcg ggcttcgcca
     2641 tagggataga aggcgatgac ctcgccctcg cggttcctct cctgcatccc ctgccagaac
     2701 ccggggtcga acagctcgcc gtggcgggcg aggaaggcgt cgcgcagcgc cccctccagc
     2761 ccgaggaagg tccgcagctc ctcggggaag acgtcgtgct cgccgaccga gaaccagggc
     2821 tcggcggcca tctcgtcctc gtgatgccgc gccgccggga agggccggaa gcggcaggag
     2881 gtcaggctcg ccagctcgtc atagtcatag aagacgaccc ggccgtggcg cgtcacgccg
     2941 aagttcttga gcaggacgtc ccccgggaag atgttggccg cggccaggtc gcgcaggcag
     3001 cgcccccagt cgtcgaccgc agatagcgca gacagcgcgg atagcgccgc cccctcccgc
     3061 tcgggacccg cctcggccgc ttcggcgagg aagacgtcca gcggcgtgac ccggcgctcg
     3121 acgtaggcgt ggcgcaccac cacggtgtcc ccctcgatcc ccacgtcctg cgaggaggac
     3181 tggaggatct cggacagcag ctcctccgag aagcgggcgc gggggaagct cagatgctcg
     3241 aactcctgga agtcgaccag ccggccgacc cggtcctggg ccagcacctc ccggtacttc
     3301 tccatcacct tctcgcgcgt cgtgttcttg gacggaggga agcggtcctt gatcaccttg
     3361 aagacgaacg gatacgaggg gagggtgaag acgtccatca ccaggccgcg ccggccgcgg
     3421 gcggggacga acaggtcgtc cgaccgggcg atgtgatgca tcaggtcgcg gtagaactcg
     3481 gtcttgccgt gcttggtgta gccgagggag atgtagagct ccgagatccg cttgcggggg
     3541 aggatcgagc gcaggaagcc gatgacctcg cgcgggctct cgacgtcggc atggaagtac
     3601 cagcgcgcga agctgaacac gacgctggtc tcgtcctcgg tgtggaggac ggcgtccacc
     3661 tcgatgccgg agcggccgtt agtcatggcg agcacgaccg gccgcacctc ctctccgcgg
     3721 cgcgcccggc cgatgacgta ggcccccttg ttgcggatga acaccgggcg cagcacgtcg
     3781 agcgcctgga tgtcctcgct gccgaaggcc tcccggagcc cccgctggac gcgctccgcc
     3841 gccagcgccg cgtccccctc gaggtcgcgg aacgatgtcc ggaagccggc gtcctccagc
     3901 accttgcgga tcacggcggc gtcgatgcgc cgcaccgcgt acatgcgggc gctcgccatc
     3961 tcccagccgc ggtaggggag ggggaagtcg tcggccacgt agtcgatggc cgggtcgacg
     4021 ccggtgcgcg ggaagaccag ccgggtcagc gagttgaaga aggtctgggc gagctcgaag
     4081 tcgttgcggc cgatgatctc ccgggtgtag gcctccttga gggccgtcca ggtctcgcgg
     4141 tcggcgaagc ggtcgcccag cggctcacgc agggcgtcgc aggtgcggtg gatcgcccgc
     4201 gggtggaggt ccatccgctc caccgtgtcc tggcggatcc ccagccagtc gcagcgctcg
     4261 aagcgctgcc gcacccgctg ggtgatggcg cgcagccgga gctggtgctc gtcgaacgcc
     4321 cagtggatga tgtcggccgc gcggctggcc gcgagattcg tgctctggag gtccatgccg
     4381 ggtcttcgct cccggcgccc ttcctatgca agagggagac ctcggagagg tcagcggcct
     4441 tccctggcgg cgtcgaactc gcggtcgaac ccggggagga tcgccttgac gaaggcgttc
     4501 gacaccaggc ggacgatgac ctcccaggtg ctggtgtcgg ggtccgagat cgggcccgag
     4561 atgtcggcga cggtggcgac ctcgtcgcgc ggccggttgt ccagaatgtt ggccaggccg
     4621 cccgccacct tctcatagat cttcttgagg accggcttct ccttgtcctg ctccgggtcg
     4681 tagaccttga cgtccttgat cagcggcttg acgtagccgt cgatccgccc gttcttgacc
     4741 ttgatctcgg agtagacgga gaaggtgccc tcggcgacgt ccagcttgcc gtaggcgcgc
     4801 agcagctcgt tcatcgacgg cagcgaggcg ccctcgatcg ccacgttgag gtcgaagtcc
     4861 ggcccggagc cctcctcccg gaaggtggcg gtggcgctcg cggcaccgct ccccatgaac
     4921 cggccggtga gcttggcggt cgccgggccc tcgcggaagc cgctcgacac gttggttacc
     4981 gtaaggtccg cgccgtcgac gaacacccgg tacgggcggt cggcgttgcg gttgaccagg
     5041 ccgaggacgc tgtcggcgag ctcgaggcgc tcgatgtgta ccggcatcgg cggctggtcg
     5101 tcctgggcgg cctcggagac ctcggcagcg cgccgcttct cggcgccggc ggtggccgcg
     5161 ctgtgggtgt agtcgagctt caggccctgg atggtgacgt cggcgatccg cgcctcccgg
     5221 tgcttggggc tgtactcgaa ccggccgcgc gagcccagaa tgccgccgtc gacgtcgagg
     5281 ttggcgcgcg agccgagcac gtcgaggcgc tccagcggca ccttctccag ctcgtagacg
     5341 acgtggaagc cgggatgcgg cttggacagg aagtcggcgt gcccctcgat gacgccgcgg
     5401 ccggtgccga agagcacccc gtcggtacgc accggcgagg ggtagacccc ctcgtccgag
     5461 cggatgttgc ggatgttctc ggcggagagg ttccagcggg tgatctcgag cggccgctcc
     5521 gggtcgtggt cgacgtaggt gatggcgccg tcccgtacct ggacgaggtt gaacttcaag
     5581 ggatagatcg actgcagcgc gtcctgccac ccgcggtcct cgacattgag ctcgtcgcgg
     5641 tcctcctgcc ggagctgcgg caggttgatg tggatgcgcg gctgttcgaa cacggcgttg
     5701 gccaccaggt ggaggcggag cagctcgcgc cactggacgc tcgccgtgag ccgcgggatg
     5761 tccgccaccg ccggctcggg gtgggcctgc tggcggatga cggccccctc cagggtgagc
     5821 gccagcccga acgggttgag gtgggcgccc gccagggtga cggtgtagcc gtgcagccgc
     5881 tggttgatct tggcttcgag ggtgcggcgg aggtattcgt cgaaatgctg ggcgatgacc
     5941 gcgatcagga ggaggacggc cgccgccagg acccacccga ggagggtccg ctgggtgacc
     6001 ggccgctcgg ggcgcgtctc gcggggaccg gagtcgctga tccgccgcgg cccctgccgg
     6061 accggctcgt cgctgtggtc ccgctcggct tcagcgggtt tgcgtggcaa caactgcatg
     6121 acatccaccg gcgcccacat gtgcggggcc ggccatagag aaccagcaat atcgttgcca
     6181 actctccttt tccttcttct gtaggggcgg ggcttgcccc cgcccgggaa ggggcatgcc
     6241 ccgccctgca tcatctatat agaatggggc catggacggg acccgcagac aggacatccg
     6301 cccgggcctg gaggtcgaga tcgtcctcaa acaggatcag gcgaccggca agaggacgcg
     6361 cggcattgtg cgcgagatcc tcaccccctc gccgacccac ccgcacggca tcaaggtgcg
     6421 gctgaccagc ggcgcggtgg ggagggtcaa ggcgatcctg acgaagggaa aggaaacccc
     6481 cgggccggcc actacgtaga gaggccccgg ggggatctct ccgtgacgac catgaccgca
     6541 tccgaacagc cggcccaacc catcctgccc ctcctggagg agggcgccgg cgagatcacg
     6601 ccgccgctcc cctccatcgc tgacggcgtc gagcacctcc tcgacccgcg ccacgtcacc
     6661 ctctcccgga tcgtccgctg gctgggggcg ggggcggtct cgctgggctc actggtgccg
     6721 ctgggctcca tcgtcttctt cgcgtcgatc ccggggtggg tgacgggcat ccttcccttg
     6781 ctctgggttg tcggtacgct gggcctggcc tggagcgccc accgctggcc cgtgatcgag
     6841 caccggcatg cttcctataa ggtggacgac aacggcatcc agatccgcca gggcgtcgtc
     6901 tggcgtaaag agatcaatgt cccccgctcc cgggtccagc ataccgatgt ctcgcagggc
     6961 cccctcgagc gccgcttcgg gctcggcacg ctggtgatct atacggcggg gaccgatcac
     7021 gcgaagatcg agctgcacgg gctggatcac gcgacggcgc tggcgatccg cgatcacctc
     7081 gtgcatgggg aaggcgatgc cgtctgaacc ggccgactcc gcggtggccg accggcggct
     7141 gcacccgctc tccatcttct tcggcttcgc ggcgcagatc ccaaagctcg tcctgccggg
     7201 agtcttcgtc ctcttctcgg cccgctcctc gcaatgggct gagtggcagc tctgggcgat
     7261 gctgctcctc atcccctaca ccctcgtcgc ggtcggccgg tacatcaccc ttcgctaccg
     7321 ttacgagccg cacgagatcg tgatccgcac cggcctcctc ttccgcaacg agcggcacat
     7381 cccctattcg cgcatccaga acctcgatgc cgtgcagaac gtgctgcatc gcctggccgg
     7441 tgtggtggag gtgcgcgtgg agacgggcgg cggccagaag gccgaagcga cgatgcgggt
     7501 gctgccggtc gccgcgtatg aggagatgcg gcggcgggtg ttcgcgggga aggcggcgga
     7561 ggccgtcgcc gagggggagg cggccgaatc cgtccccgtc ccggcgtcca cgccggaacg
     7621 caccctcctg cgaatgccgt tgcatgatct gctgatctgc ggcttcatcg agaaccgggg
     7681 catcgtgatc atcggcgcgg gtttcggtct gctctgggaa gcggggctga tggaccgcct
     7741 gatgggccgg tggctccgcg tcaacgggcc gggcggcggg ttgccggagt ggttcggcgg
     7801 cgatgccccg gggggcgggt tgtcggccgc cgtggtcaag gcctttctgg gccagggcga
     7861 gctccccttg acccggatcg cgctggccct caccgcgttc gccggcatcc tcctgctcat
     7921 ccggctcctg tcgatgatct gggcgttgct ccggctgtac ggcttcagga tcgtgcgcgc
     7981 cggcgaggac ctccgcaccg aatacggtct cctgacccgg gtcgtcgcga ccatccccct
     8041 gcgccggatc cagacgctga cgatccgcga ggggccgctg caccagctct tccggcgcgc
     8101 cgcggtgcgg gtggagaccg ctggcggagg tgggggcgga gatggtgagg aggaatccac
     8161 cgccaagcag cgcgagtggc tggcgccgct catccatcgc gacgccctgc cgggcttttt
     8221 gcgcgaggtg ctgccggagg ccgatctctc gacggtcgaa tggcggcccg tcgagccccg
     8281 ggcctttcgc cggcacctca agaagtcgct catcgccgac gtcctgatcg cgctgccgtt
     8341 cgtcctgatg ttgaaattct ggaccctcgc cctgctcgcc gtcttcatcg cctggtccgt
     8401 cgtcgccgcg cacctctacg tccggcatct cggatgggcc gtgatcgatg gcgcggtcct
     8461 cttcagaagc ggctggctct ggcggagggt gagcgtcgcg cgcttcgcca agatccaggc
     8521 ggtgacgatc cacgaatccc cctgggaccg ccgcgccgcc atggcgcgcg tccgggtgga
     8581 caccgccggc gcccgtggcg aggcgcagcg cgtggacatc ccctacctgg cccgcgatac
     8641 cgcgcgggcc ctctacgagc tgctgtcggg ccaggccgcg cagacggcgt tccggtggtg
     8701 atagccgatc gttgactttc tgtaatgatt aatatataag actggctatt cggcccgcct
     8761 ctacggtgtc tgaaagagag gaggagccat gaggccgcag tctgtccggg gacctccgga
     8821 gcactcccga tcccctcgtc gttccgccga gatcccgatc gacctgtccg ttctttggag
     8881 gtaccgacat gctgcttctc tacgacagca aatgttcgat ctgcaggagc ctcgcctaca
     8941 agatccactt cctgaccgag agcaatgtcg agatcgagtc gctgcacagc ccgaacgcgg
     9001 agacggtgct cagccggttc tacccgcaag gttggcagca tgacttctac gtcgtccaca
     9061 acggcacctg cagaaagggc ctgcgggcgc tgccgagcct gatgcgctcg gtcggcctga
     9121 agcgcatggc gtcgctggtg tcggagtatg gttcctacaa gcttgctccc aaggcctgcg
     9181 gcgccactga tgccaacggc aacggcgacg gcgtggtggc gccgaaacgc aacttcctga
     9241 aactggcggc gatgacgccg gtggcctacg gcctttcgca cctccggctg gagaaccctt
     9301 tcgaggagcg tgacaggccg aagctggacc tctcggtgca cgtcgcggag gtggagatga
     9361 ccggtccggg cgagttccgg gtcaaggcct accgctgcga caactgcggc cgggtgccgg
     9421 cgccggacaa ggggctgccc gcgggagcct cgaagcgtct cctggaccag gtgatgctcg
     9481 ccgaggactc catcgacggc tttgcgcaga aggggttggg gactgccaac ttcaaggtgc
     9541 agcgcatcga gtacgagcgg gagatgcccg agaacgggag cctctcgcgc gagaagcggc
     9601 tggtccacgc ggcgctcctc gaccatccgg agtacaacct ctcggtcaac atcggccagg
     9661 ggcggacgac gatgctcgcc ggcatggcgc ggcacgacct gccgctgccc atgctcgact
     9721 acctgatctt ccggccggac accgaagagg acgtggtgac ccacctggcc gcgtaccggt
     9781 cgggcctcct ggagcttgcc aagctgcacg gccggcaggg ccgccaggcg ctcgcccggg
     9841 tgtaccggga gatggcgcag ggattcatgg tgatgagccg gcggttcgac gcggccgtgc
     9901 ccgagcgcct gttgccggcg aagaacgaga tcgtcgtcac ctccgtcccg gagctcctcc
     9961 ggttcgttca gaagccgcct gatctccagc actcggaaaa ggcatgcgac tgctcgtgct
    10021 cctgcaacgt ctgctgcggc tgcggctgca gcttcggcgt ctgcatcgag ttcgtcagcc
    10081 cctgtggctg tgactgctgt ctcgcctgtg gttgcggttg cggttgctgc ctgtagcgct
    10141 gaagacctct ctccgggagc gcggccagcg ccgcgctccc gcacctcttc cgggcgtccg
    10201 cccggttccg cgccctacgc ctattcagcc gcttcgagca tcgcgcggat ctcgggatgg
    10261 gtggccgcct cgaccagggt ctgcttgtgc tcgccgagct tcgcgccgga cttgagcagt
    10321 aggcggacga cctcggggtg gttctcgttg accggcccga agatcagcgg gaagccggcc
    10381 ggagcggcgt tcggatcggc gccggccttg agcagggcct cgcacatcgc gaggttgccg
    10441 acgcgcgccg cctcggagag cggcggcacc tcgtagtcgt tggccgtctt gacgtcggcg
    10501 ccggcctccg cgagggcggt gacgaacgcg gcatccccgg aatcgaccgc ctcgaagacg
    10561 agcgacttgc ccccttcccc catcggcgcg ttcttcgcgt ccggcttcac gccgaaggcg
    10621 agcagcgact ggaggaccgc gagctgccgc cgctcggtgg cgacccacaa cgccgtgcgt
    10681 cccgaatcgt tccggacgga ggggtcgatg ccggcgtcga ggaagagacc cacccgcgcc
    10741 acgtcgccct cggcgacctt ctccaggaag gcctccggcg tgaacgggat cccctccttc
    10801 tcgagctgag cacgggcgcc ggcctggtcg ctccaggcgg gaggcgcggc catcagcagc
    10861 agagaggcga gcgagagcac ggccgcgacg atgagggtct tcatggggat ctccttccat
    10921 tggcaagcta tccgagaacc ctcggaggtg caagcggagg gctcgggcga ggctccgtag
    10981 cgcttccctg cgggagggaa gcgctacgga gcgacttcga agcgttgagg agcggcaccg
    11041 tagtgtttcc ctgcggtcgg gaaacgctac gaagcgactt tggagccttg aggagcggcg
    11101 ccgtagtggt acggagccgt ccctcaacgc ttccctgcgg gagggaaacg ctacggagtg
    11161 acttcgaagc gttgaggaac gacgtcgtag tggtacggag cggacccgta gcgtttccct
    11221 ccgaaaggga aacactgagg agtgactttg aagtgctcag gaatggcatc gtagcgtttc
    11281 cctccgctcg ggaagcattg aggaatgact tcggagcgtt gaggagccgc gccatagtgt
    11341 ttccctctcg gagggaaaca ctatggcgcc acgctgaagt attgaggaac ggcgccgtag
    11401 cgtttccctg cggccgtgaa acggtgagga accgccatag agcgtgaggg aaggagcggc
    11461 gggggctccc gtgcccggga ggaaaagatg gaggagatgg aggagagctt ctaagcctcg
    11521 ccggagccgt tctccggcgc cgccttacgt ttccggccgc ggaacggctg catgccgaac
    11581 gcggccaacc tctccgagtc cgggccgtag tgctccgaaa ttccggtccg cagcagcgcc
    11641 gccgtccggt ccgcctggtc tctgaggccg ccgaattcgc gagtcagctc ctggcgctcc
    11701 gcctccagag ctgcctgccg ctggatgatc tcctcggccc gttcgagagt ccctttcaac
    11761 ttgccgcgga gggattcgag gtgcggaagc tccccggcat gagcctccaa gggaggaagc
    11821 aaccgccgcc acctgtccaa cgtctcgttc ttctttggag gcatgcctct ctcctttctc
    11881 ggtggtccag caaggacgct gggcctctct ccggttctgc gtgggagggg gagatcttac
    11941 ctctgtaggc ggaagaggga taggggcgtc tcacgagggc agtgcttctc ggagtctgtt
    12001 gacaagccta cagggccggc gtagcattta gacggcatag atgaagccgc gaaagcgata
    12061 ggatctctct ccggaataac agggtaccaa gatgatagat tcttccaata cccatgagcg
    12121 ctatgtcacc gatggagccg gcaaccggat tggcgtcatc ctgaccctgg atgagtattc
    12181 gcaactgatg gaagccgccg aagagttgga ggccatccgc gctttcgacg atgcaaaggc
    12241 cagtggggat gaggcgatcc cgttcgagca ggcgggccat gaagtgcaac ccggggagcg
    12301 agatcctctc gggaatctcg tgggtctcct acgccaccgg gccaacagcg agccggtgag
    12361 cgtcgaggag atgggcgagg cgatccgggc cagggcggcc gagaagtatg gtgaccggaa
    12421 gggcggttga ctgtattgac ggagttttgt ttgagggaag gggtcaggtg gagctctcaa
    12481 gtcgtctgcg cgaaatcgag cacgagatag attccaagat tcgcggtctg gaagtttggc
    12541 aagcgccgag agccgaagtt cttgagcatc ttgtatatac atatcgagac ctcattgaag
    12601 aggccttcat gcggctactg catgcgagga ccttcggcct tccgcctgac gaagccttct
    12661 accttgagaa ccgggggaga aacggtatgc tctgggcgct taagtgggct tcggaatact
    12721 gcccggagag tggaaaagcc cacctgaggt cgcccgacga tctcatggag ttagtgcgtc
    12781 ttggtggagt gtacgaggtc ttcgtagatg ctttgaagta cgccggtcga ggtctggtca
    12841 ccatccatgt ggaggaatcg tccaaaatca ttacctgtta tgaaggctcg ccggctacag
    12901 catttgatgc gagtatcgtt gatcgagaga ggattgtctc acctcagatg ccgcatgtgt
    12961 cattgactga ggatggtgat cggataacaa cgcgatggac agcaggggat taccgcaaga
    13021 taacaaaaga gttggcgcag tatggggcaa gcgaagagaa tgcaattgtt gtggacccag
    13081 aatttgcctt agcagctggt atgtctaatc tctcggtgcc gcagccgaca gttgtatggg
    13141 tgaatcgacc cgccgacgcg ccgggctgcc atgtttttga tgatcttgtc ttgccggtcg
    13201 ttatggattc caaggtgaag tggcagctgg tctcgttgct tgaaaatccg gtaatcaagg
    13261 ttggggagag atattgcgcc ttgtcatccg atctgagagc gatatcggag attgacgact
    13321 acatgctgcg gctggcagct agggttgacc agcggcagta tagtctcgct tccgttcggc
    13381 gggaagagcg gatgctcgcg atctgccgac gtgcgcttga acaagcttcg ccgccatggg
    13441 ctgttgaaga acaagtgcgt tataagaagc ccactcaaga ggccgacatt ctcgcgaaaa
    13501 gaggcgctga taaaattgca atcgagctga agtcgaccct tcgaccagag acaccgtggg
    13561 aggtctttaa gagaaatcaa gacctagtca agggcgtgaa gcaagtcaag aggctgatcg
    13621 ataggtccgt cgccgaacgt ggatttctag tgacggatgg gtatcgaggc gattatgtct
    13681 gttgggcaga ggctttggct aacgaggttc ccatcggaaa cttatatgat ttggatatca
    13741 ttgccgaaga tccgtctggg gcgctacgcc gcttgaagga tctggttggc attccgaccg
    13801 ccgatattag gatacctgaa ggcctccccg accgagaaga aaatctgttc gagtggacgt
    13861 tgcgactggt ggataggaaa tcgccggatc atcctggcta agatctcttc tcggcggtat
    13921 tgaggcgagg gcggctgagc ctggttagtc ctcgtctgga ttgaaagatt cctcctgtcc
    13981 ttcgctaacc cgagaggctg tcttaagtac ccgggcctcg gagactacga gccctccccg
    14041 agccgtggca cgacgaacca ctgcaataaa ggcttcatca tggtgccggg cgatgatgct
    14101 gcggacggac tttaaggccc ccaggaacag gtctgccagg tctctggcga cgaggctgcc
    14161 tcggatgatg aacagcttcc cgccgttctc aagggtcgcg cgcacggcaa ccgtgtcgcg
    14221 ccgtatattg ctgtcgtgcg agagtcctat ccagccacgc cgagcgataa aagacaacca
    14281 ctcctcgtcc ggcacgttcg cttcctggaa gtgatcacaa tagcgttcca ccaatagccc
    14341 accctccctc agtgcgccgg gaacgatctt cccgaggtca cggtcggtga agaagatggg
    14401 ctcaggcagc gaggggcgct tcgtaccgga tggcctcttc aacctcgaag acctctaggt
    14461 catagtcctc tgccagttca gagatggact cgccggcagt gaaacgctca gcaatgatcg
    14521 aggttttgat ggctttccgt tcaacgaccg gccggccgaa ggcgatccga gggttgatgg
    14581 cgaggagcct cggcgaatgg ggctggtccg ggcgggtcaa ggggaacatg cggatgggcg
    14641 cccccgccac atcccattca atgcgttcca ggtaggcgct caggatctcc ggcatcgcgc
    14701 cctgcccgcc acggccgacg ttgacgaggg agtcgaggtg ctgaagaaag acgttacctc
    14761 gggtcgcccg tagctccggg ctgaggagga ctctttcgat gccgaactgt tcctgagcgt
    14821 aggcgagagc cgtccggatc tccttcatct tcacctggta ctgcttgcgc agagcgagga
    14881 ggac
//
LOCUS       DASPJI010000637        25370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_267679, whole genome shotgun sequence.
ACCESSION   DASPJI010000637 DASPJI010000000
VERSION     DASPJI010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 25370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25370)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25370
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267679"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VEL74_17855"
     CDS             <1..262
                     /locus_tag="VEL74_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64448.1"
                     /translation="VDTRHVGDFTVWIGDGMVDWNQPVTLVVDGKKVWEGDLDPDLHL
                     CLAQAARTRDFDRLRWAGLRVDDDRRVSVVTAETELPSLLPD"
     gene            624..1211
                     /locus_tag="VEL74_17860"
     CDS             624..1211
                     /locus_tag="VEL74_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64449.1"
                     /translation="MFAAFVLLLTASTLPLAAVTVRQMNLEEMVDNADKIFRGTVLDV
                     RETTVNAGGGELPVVVYRLRVDESFKGSYQTVKGIQVAEVRMLGKLKTATSTGSKVRF
                     QGFIPELPNLQVGHDYLLLTTASSAIGLSTTVGLGQGRFELSGKAGQEVAINGNNNRG
                     LFGDPLAEGSSRAAAPPEGPLPYTFLANTIRGLVG"
     gene            1226..3934
                     /locus_tag="VEL74_17865"
     CDS             1226..3934
                     /locus_tag="VEL74_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64450.1"
                     /translation="MKRTMFRSFVFAAATALLSQAAFAAGPLEICNPGQPYRWAAGGV
                     NIPWNPDQGGLGPLTNAQAIDLVADAFQGWGDIPTATTTYLQGAALAVDVDDTNFVPY
                     FFSDAPDGVSAIVFDEDGAIFDLLFGPGSGVLGIAGPEWGDIPTCTITEGFAFLNGGA
                     FGDPVEAFDVTFHEFGHYHNLAHSVVNGQIAALGDYSGPTPHDTFPIGSLVNLIETMY
                     PFYLGPPAGMGTPHRDDIASLSQLYPTASFASTTGTITGRILGPNGTTQLTGVNVIAR
                     NVADPFADAVSAISSDFTDTYTQGAPGVGVYTLRGLTPGAQYAVYVDEILAGGFSTPP
                     LFPLPGPEELYNGASESSNGATDNPSAFTGVAAAAGATASNINIIFNAPAPNTPLPVG
                     DDSSVELFLPFTFEICGQPFNSVYVNGNGNLTFGVPNEDFSESIDEFLAGPPRVAGLW
                     DDLNLDGGGSVFFQQSSNTFTVTYQNVPEYPATGANTFSIQLKRSSNHIDINYGTLTA
                     TDGLAGVSCGGAITSGFERATNLTSDNGTNDLKKQPAIFELFGPGNLNDLSGRSEKFN
                     AKTRFADEFEHNDTLAHAEDVRLPFHTEDVDDFTEISPEGDDVDFFEFRVKEGDILAI
                     EVVRGNLDTVLGLFDADTGDLLLADDDGGNGLLSRLLVQVPAGFDELAVAVSTYPDVD
                     FTGAGEGSGRYVLQITKYEGTVLAAGDDTSTPLPLGFTFPYQGGQYTSVFVNSNGNLT
                     FNAGDADLSETVPEFLAGAPRIAPRWDDLDATGGVIVASPDDDDKMTIHFVSVPEFFS
                     TSPNYFSVELDEDGDIEFEYLANSRTDGLTGITKGGGVPDPGEDNLSRDDEHPATGTT
                     YELFSTADVFSFDLFYSDVKFDEDDDDDDDDDDDDHDDDDDDDDDD"
     gene            4215..5102
                     /locus_tag="VEL74_17870"
     CDS             4215..5102
                     /locus_tag="VEL74_17870"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYG64451.1"
                     /translation="MPILAFLIACGTGPAATAPADTRPFDYDSKAPLDTRVVSTESRD
                     GLTIQEIEYASPRGGRVPATVMIPAGKGPFAGILLMHGAPGSRRQTFKEAEELARAGA
                     VTLSIDAPFARQGRPAGEILRFDEQDREDSIQLIVDLRRGVDVLLARSDVDPKRLGYV
                     GGSYGGAMGGLLAGVEKRIKAYALFVGDGGLVSHFTGADDTNGPLQQMPKDRADRWLA
                     AMLPIEPIHFVGRAAPAHLLFQNGRQDTMVPPADGRAFQEAGSEPKTIQWYDSGHKLP
                     DEARAARRLWLAERLGLKG"
     gene            complement(5166..7118)
                     /locus_tag="VEL74_17875"
     CDS             complement(5166..7118)
                     /locus_tag="VEL74_17875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HYG64452.1"
                     /translation="MRKVFVRTGLAVVLATLSVAAPVFAGGNIEVYDVTGFVPSPIPG
                     HIIGKNVPVHWDSRCIPVHFRVNDTLNPIPNPLGTPFLSVAAVTPVIQKAFDSWNRIP
                     TSFIDMRVVGTVPNPGFAAFDGINEVTFRTPAFGAIAISSPYSLIYDLTLFPGDDIDE
                     DGDSDVAATIATCADVDNDGDIEFPAGFYEAGTIFENDVEFNTTAARGYRFTINDDEA
                     DTVQRSLDFEAVAVHEFGHSHGLSHSVIDQVSDTDGDGATMFPFVNPTLPAHELAART
                     LEEDDIAWSSYTYPEGTAASGLAALQPGDVAFHQAYGLIQGSVTHGGLSQPVAGAVVR
                     ASATQGEQKIFSSTFSGTTQVSVDEITGEEFILSPSFNILDGNYVLPVRKGVWNIGIE
                     PLDGRPVGAGSFNTTALIGAVFGQQSFNEEFFNGPGEGALELRSGQAIPQVAVPGLPR
                     TGVDFITNRDIQIANFGDVENFVSGNAPAGLYYAVRVPAGQITGAVPAGEDVLFHAMQ
                     FDTLVVDSSTVPVFAEAMLATGTVNGSTATIDLAHPLAREMSFVGQDNDLSPAYFQSP
                     VALGSIVQNGIQHGTIKDLFLVLRLPTTTPFPGVAGIPPLVGTDRGDDGPIYGYSYSS
                     TDGVTFTQEPDLNYRFSLVLSRKPTP"
     gene            complement(7115..7693)
                     /locus_tag="VEL74_17880"
     CDS             complement(7115..7693)
                     /locus_tag="VEL74_17880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64453.1"
                     /translation="MQKVLNALAGTAFVLAALPAVSSATTVVEVTPEDMMRQAQVIVA
                     GHCVHKESAWVGRDLVTLATFSVSEGFKGAEAGTQITVVLPGGVDLKRRVPIAKTYPA
                     APQLGDQEQALLFLTPETRVPDGYMVVGYSQGKFTLVTGPDGKKMAAQDLGGLSLVGK
                     SGGLGHGNSRAIPYDQFRRQILDAVAGRRSEP"
     gene            complement(8161..8697)
                     /locus_tag="VEL74_17885"
     CDS             complement(8161..8697)
                     /locus_tag="VEL74_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64454.1"
                     /translation="MRKNLILALSLMMASFIAAGAAESPAGAPDPGLEAMKAVAMMQG
                     SWEGGGWMRMGPGEPERFVGEETVEARLDGRVLLVEGRHWTPDRSRVVHHAFAVLTYD
                     PAKKGYRFSTHLGNGRSGDFPGRVEDGAFIWEMETPGGRTRYTIRIEGDRWHEVGHIE
                     RQGQWTQFFEMELRRQGK"
     gene            complement(8764..9546)
                     /locus_tag="VEL74_17890"
     CDS             complement(8764..9546)
                     /locus_tag="VEL74_17890"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG64455.1"
                     /translation="MSYVEIPPPAELHPWVAAFWCFGVRAGAGEIEHRIPLTGGLLLS
                     VDRSGLPMLAGPRTAPLVTTVRGGDLFRGVHFHPGGARPFLHLPPGSLRDAMGPAHLW
                     LDRAWCGSWGPVAGGEDDMAALDALARGLRELMPRAEPPDPVVRSAVDSLRRSLRTEQ
                     ADQGTRVEELAAEVRLSPRQLRRRFQAAVGLSPKELARVLRLRSSAASAVLGVRPWAD
                     VSAEGGFADQAHLVREFRSLLGVTPGRFEQHARRIDHRLLPE"
     gene            complement(9624..10532)
                     /locus_tag="VEL74_17895"
     CDS             complement(9624..10532)
                     /locus_tag="VEL74_17895"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HYG64456.1"
                     /translation="MHFLITGIGGFLGTRLARHLLAQGDRVSGTFLETRPDFDGVDLF
                     EADLLDTAALRRAVDESAPDVVVHLGALSHVGESWKRIPDYFRVNVLGTENLLAAVGR
                     RGGRRVLVASSAEVYGIVPDERQPIGEDEGLDPRSPYALTKAAAERLALAAGAVVVRS
                     FNLVGPGQSPIFALPAFAMQLAAISRGARGEQEPVLRVGNLSARRDFAHVDDGVAALR
                     LLAEKGEPGGIYNISTGKAVSIGEALERLMAVSGVQARLETDPERIRPVDIPLLAGDP
                     SRLRALGWEPRRTLDDALADLWETVR"
     gene            complement(10570..11544)
                     /locus_tag="VEL74_17900"
     CDS             complement(10570..11544)
                     /locus_tag="VEL74_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HYG64457.1"
                     /translation="MRALITGITGFAGSHLAEYLLAEHPDVEVFGTYRWRSRMENVEH
                     LRSKVALLEADLRDYTSMHAALEQSRPDYIFHLAAQSFVPSSWKAPNDTLVTNVSGQT
                     NLFEAVRALKLDPVIQIACSSEQYGLVLPDEVPIKETNPLRPLSPYAVSKVAQDYLGY
                     QYFQSYGIKVIRTRGFNHTGPRRGQVFVTSNFCSQVASIELGLQEPVIRVGNIDAIRD
                     FTDVRDMVRAYWLAVTKAKPGEVYNIATGSGISIRDLLDKVLALSNVKVDVQVDPDRL
                     RPSDVEILIGDSSKFREDTGWEPRIPFDQTVRDLLDYWRETLAHRKRS"
     gene            complement(11544..12407)
                     /gene="rfbD"
                     /locus_tag="VEL74_17905"
     CDS             complement(11544..12407)
                     /gene="rfbD"
                     /locus_tag="VEL74_17905"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HYG64458.1"
                     /translation="MRALIFGGTGMLGRAVVREARARGWAALALSHVQADVTDRGRLA
                     YWADTFNPELVINCAAFTQVDVCETEPERAYAINGEGVGNVAAAAGRAGARLLHVSTD
                     YVFDGAGREPYREDAPVAPMSVYGRSKLEGETRALEHPDALVVRTSWLFGPGGPNFVR
                     TMIRLMNEGRLPLRVVDDQRGCPTYTPFLAQALLDLAPLSATGVVHYRNREAATWYDF
                     TVEIARLWRGPSGPADVVPVTTAEFPRPAPRPAYSVLDVSRFEHLVGRRVEPWEWGLV
                     EYIAAMRQGRT"
     gene            complement(12427..13503)
                     /locus_tag="VEL74_17910"
     CDS             complement(12427..13503)
                     /locus_tag="VEL74_17910"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HYG64459.1"
                     /translation="MKGLILSGGKGTRLRPLTYTSAKQLVPVANKPVLFYAIEAIVAA
                     GITEIGIIVGDTREEIRAAVGDGSRFGARVTYIEQDAPRGLAHAVMIAEDFLGDSSFV
                     MYLGDNLIAGGITELVDEFRSQDCNSEILLAEVPTPEQFGVAELTPEGKVHRLVEKPK
                     EPKSNLALVGVYMFDSTIFESVKRIRPSARGELEITDAIQDLIDRGLAVHPHIVRGWW
                     KDTGKLDDMLEANRIVLESLDARRGVTLGDIGERSRIEGRVEIGEGVVLVDSLVRGPV
                     VIGDGARLEHAFVGPYTSIGERCTLVSCEVENSIILADSEIRDIPLRIDGSLIGRNVR
                     ITKTDFKPKAYRFMLGDNSEVGIT"
     gene            complement(13540..16101)
                     /gene="lptD"
                     /locus_tag="VEL74_17915"
     CDS             complement(13540..16101)
                     /gene="lptD"
                     /locus_tag="VEL74_17915"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HYG64460.1"
                     /translation="MLDTRRPLTGLLIAALCLTLPASGQEPAQPQAQPLPAQATAQTT
                     APAQPAEQTAQPMAPPTDPAATPPATPVTPPTSEQQPDSPSVPPMPPQTLPEPQGGGI
                     VPPAVPAVPGAGPDRIEFNLNFPADQGGGSAAGSAGSLEYQRDDYAVLSGGVRIRYQD
                     VNLQAEQAEIDLNTKIVTARGNVVIDQGPRRLSGDTANYDLDTKTGRLTNATANVSQD
                     YYFSGSEVAKISDTVYTVEDGVFTSCSQEVPDWSSRLGSARVEEDQYAHVRNASMRAK
                     KLPVFYTPYILWPVKPDRSSGFLIPNIGYSDRRGASVGLAYFQTLGRSYDTTFHVDTY
                     TESYLGLGNEFRYRPSEGTRGNLLGYVIRDPESVTDEEWRWKLEWNHETRDLWRGMRA
                     VVAFQDYSDFNFFQDFERDFDRNTRRFIDSRAFVTGNWGAHLFTLLLNDRQTFVNASN
                     ETLDERRLPELDYTLRRTRIGRTPLYMQFDGSASYLDIDRPNGFSGSYGRLDLFPQLS
                     LPVRSFPWLSLTLTGGGRVTYYQDTVDLDNRSQLSGESLLRALPSGTAEIVGPSFSRI
                     FDLNLGSFGRFKHVVEPRWTYNYLGEFEEENEVPLFDEVDTLRSTNSGRFVFANRILG
                     KPREVPAPPAPPAQPVVPGQPAPPAAPVRPVNVSAREVLYFEVSRRYSFDSEQPLEQG
                     LTIPDDPTSFQTAQAGPLEALLRFNPSDRTSVKLEADYSMLFSGLSSTSLSGNYGFRK
                     NDTIGLTWFTRFQPRTGEELDNQVRLTGGLELLPNKLRLDSQINYSLTTQLLQQQIYA
                     LSWSGQCYGVRVEMRDFRAGAGDRIRDKDFRFSLSLKNVGTFLDLTSRSSTSQEP"
     gene            complement(16111..17295)
                     /locus_tag="VEL74_17920"
     CDS             complement(16111..17295)
                     /locus_tag="VEL74_17920"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HYG64461.1"
                     /translation="MDDVVILSAVRTPIGAFQGALAGLPAHALGSRAIAAAIERAGVT
                     PQDIQQVNMGCVLTAGQGQAPARQAALGAGCPPHTGALTLNKVCGSAMRSVMIAANDL
                     RCGDFQLVAAGGMESMSQAPYLAHGVRDGLRLGHGRLIDSTIHDGLWDPYNDLHMGNC
                     AELCAREYQLGREEQDAFSLESYRRSREATEKGLFAEEIVPVSLPQKKGDPKVVDRDE
                     EPFKVDLARMGSLRPAFQEKDGTVTAGNASKINDGAAALVLTTASHAARIGARPLARI
                     LAHSSLAQKPEWFTTAPVGAARSVLERAGLKAADIDLWEINEAFAVVALAFIRDLGLD
                     AERVNVRGGAVALGHPIGASGARILVTLLHALRERGGRLGCAAICIGGGEATAMVVER
                     LD"
     gene            17411..17827
                     /gene="ruvX"
                     /locus_tag="VEL74_17925"
     CDS             17411..17827
                     /gene="ruvX"
                     /locus_tag="VEL74_17925"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HYG64462.1"
                     /translation="MSARALGIDFGEKRIGLAISDPEGRLAVPLTTLERRKDRAAVAQ
                     IAEIARREGVGRLVLGEPVGLDGRRGEAAERVRRFGEKLAAAAGLPLEMVNEALTTVE
                     AAERLREAGVDPRREPERLDAIAAQILLQEALDRRG"
     gene            17849..19006
                     /gene="mltG"
                     /locus_tag="VEL74_17930"
     CDS             17849..19006
                     /gene="mltG"
                     /locus_tag="VEL74_17930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HYG64463.1"
                     /translation="MARNTTAGKGKAKAGAKWSGFKRFLTGAGILLLLLMIPVGMAAW
                     SWLKVQRPYKGYEGAEKLVTVEPGMGASQVLDLLQREGVLADAKLARPYLIYFMGDPR
                     IQAGEYLFRGPLSTPEVLRKLAKGEVATRSVTLIEGLTLEEAARQIAGKGFGREEVLL
                     DLMRSPELIADLDPEATDLEGYLFPETYSFSRGTSEREVVATMVKTFRRLYERDVRPV
                     LARNPDGRTLRQVVTLASIVEKEAQVPTERPLIAGVYRNRLQRGIGLGADPTVIYALK
                     RLGRWNGNIRRDDLRVDSPYNTYRYAGLPPGPICSPGLASLEAAAAPADVPYLYFVSR
                     NDGTHVFAETLAEHSRNVEQWQRRYWRERRARERREREAQASGATGSPGRQ"
     gene            19173..21176
                     /locus_tag="VEL74_17935"
     CDS             19173..21176
                     /locus_tag="VEL74_17935"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HYG64464.1"
                     /translation="MNIPEEFGNYLLLKKLTEDPLGETFRAGKVGKEGMEQVVLLRVF
                     NGKGMDGEALWQKVSGRAAVQQALRSPNIGSGADLGKVRSFPYAAYDYISGKNLATVL
                     AQADRQGSPVPTDHALLIAERLALALAAAAETRVQDERVLHGFVAPHLVMISNEGETR
                     LVGFEVAPGLRALTAGGWNDEALKPYLAPEALAGAPISKADDVWSLGAILYELLTGER
                     LPATAPAGGFGALIDGAMLANDGTPLPAAVAALLKKSLAPADQRVGDAVSWHKALSKL
                     MIDGHYSPTTFNLAFFMHSLFRDEIERENREIEAERRMDVPQRVAAPVPVPAAAPAPI
                     QDFRESTGVRQGTLPGVQAPVAAQAARSKAPLFIGIAAATLIAVGAGAWVVLSGSGGK
                     ETQAAAAANTVPSAVPMNAAAQPAVMPGAMDPAAAGPAAPAGPSPEEIQAQIQAMFEA
                     RSQEMEAKLKTQYDDRIKQLQQQLQDSKRAAAEPVRQPERPALVTPPEPSPTPAEPKP
                     TLRQEPAAAAPVIPSPNLSAGSQQPAAGTQQEAARTTASQPPAPVTTAPATQAPAPRQ
                     VQTGDLVSAGSPGSVAPKIRVLPDPRYPTAAQKMKRSAEVAVRVLVDESGRVLDAERV
                     GNKAGMGFDEAAVEAARRASFQPATKDGVRVKMWTTVRIKFTP"
     gene            complement(21261..21881)
                     /locus_tag="VEL74_17940"
     CDS             complement(21261..21881)
                     /locus_tag="VEL74_17940"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HYG64465.1"
                     /translation="MKRALTLSLCAALVVAAAVPVFAAASDKPRIAVIEFKNKAENQW
                     WYHGGAGAAQDVFVTELFKSGKFRVIEREQLDALMQEKNLSLSGDVDPATAVKAGKLL
                     GVNYILTGSVTEYGVTEKGANTPGFRRLPGVSVGKKEFVAAMNARLIDTATGEIVWAD
                     EARGSEGTARVHVGGYGGGVDDDRMFDKVLKPVIQQLVASIKATDL"
     gene            22008..22718
                     /locus_tag="VEL74_17945"
     CDS             22008..22718
                     /locus_tag="VEL74_17945"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HYG64466.1"
                     /translation="MIIVADGSELLCITQPDHAHLSGELMALWRAGGVPEHPRRDDLV
                     FAAREHDNGWREADAAPRWDAARDRPHDFVTIPREDRIAIWERGTARFAGARPYASLL
                     ITRHALTLHRDRRGDAAWDDFLGSMDELERALFEETGASPEEVAADYRLIDLADIVSL
                     AACNRWSEPFERHGVRGSFADGTMRLDPLPLAGATTFTVPCRRIPHRSYGGDVALGVA
                     LATARWQELRLRIAGAEG"
     gene            complement(22730..23194)
                     /locus_tag="VEL74_17950"
     CDS             complement(22730..23194)
                     /locus_tag="VEL74_17950"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG64467.1"
                     /translation="MGMFVNLGRTLSLLRELRGKSQACVAREAGIGKSQLSKYENGKE
                     LPKLDSLEKVLRTLEVGHFEFFYTLYLVDRRASDLLRLGGDGSGEAKEPPELRDEHFC
                     IPPLLKGHPLLSESTDRAFTRVFADLLALYRMVFEQIVLSGAGRPEDLKIPS"
     gene            complement(23460..23990)
                     /locus_tag="VEL74_17955"
     CDS             complement(23460..23990)
                     /locus_tag="VEL74_17955"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG64468.1"
                     /translation="MFDLIGETLRRLRKEKGKTLEQLGAEAGLGRGQLSRIENARQEA
                     TLSTLAKILQSQGVGRREFFRRYDLVEAEALALQGPAGAPPQAVEGPAVEEGEGRWAE
                     DIRQALGRVGSFVQSTFSQSRPIAQGAVELGEVVVLFQVVPKALHRPDPVPEGGRPEG
                     GEAPAPSTSQRRKKKR"
     gene            24354..>25370
                     /gene="crtI"
                     /locus_tag="VEL74_17960"
     CDS             24354..>25370
                     /gene="crtI"
                     /locus_tag="VEL74_17960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HYG64469.1"
                     /translation="MTVIGAGPGGLAAAILLASAGLRVTVLERLPRAGGRTSTLEADG
                     FRFDLGPTFFLYPQVLRDIFATAGYDLDREVPMVRLDPQYRLVFGEGGELLATPDLER
                     MERQVAALAPADAPALGRFLADNRRKFERFRPCLEEPFLGWADLLNPRLLKLLPDLKP
                     WRSLDSELGSYFSDPRLRLAFSFQSKYLGMSPFRCPSLFSILSYLEYDYGVFHPLGGC
                     GAVTEAMARVAREIGVDIRLDEPVREILFEGRRAVGVRTDRGDYRSDGLVVNADFARA
                     MSRLVPDHLRRRWTDRRLEKKKFSCSTFMLYLGIEGVYDQVAHHNIYVSADYERNLDD
                     IENRH"
BASE COUNT         3989 a         8941 c         8616 g         3824 t
ORIGIN      
        1 ggtcgacacc cggcacgtcg gcgacttcac cgtctggatc ggcgacggca tggtggactg
       61 gaaccagccg gtgacgctgg tggtggacgg caagaaggtc tgggaggggg atctcgaccc
      121 ggacctccac ctctgcctcg cccaggctgc ccgcacccgc gacttcgacc gcctccgctg
      181 ggccggcctg cgggtcgacg acgaccgccg cgtctccgtc gtcacggccg agacggagct
      241 cccctccctc ctgcccgatt agcccgggaa gaggccgggg ggtgtcccgc tcccggcctc
      301 tcgcgcacca tccctagtaa gggcagcgat ccagtccaga cgaagtttct ggaattgctc
      361 ttcgtctaac gaacagatcc accggtttgg catggggaac tcgccgcgag gcgaagacgc
      421 caggccgggg atcttccgca gggtcgctac cgatgggggc cagattctga ctgtacaggc
      481 tgagaaccgc cttccgcgga gttccgttcc gcaccttcct cccggccggg ccccgcccgc
      541 cgccctccag gtctgcgtca tccgacgacc caagaaacac aaaaatgggg ggtattatgt
      601 accgtttcaa aaataaaaga agtgtgttcg ccgccttcgt cctgctcttg acagcctcca
      661 ccctgccgct cgccgcggtc accgtccgcc agatgaacct ggaagagatg gtcgacaacg
      721 ccgacaagat cttccgcggc accgtcctcg acgtgcgcga gaccacggtc aacgccggcg
      781 gcggcgagct gcccgtcgtg gtctatcggc tgcgcgtcga tgagtccttc aagggttcct
      841 accagaccgt caagggaatc caggtcgccg aggtccggat gctcggcaag ctcaagaccg
      901 ccacctccac cggctccaag gtgcgttttc agggcttcat ccccgagctg cccaacctgc
      961 aggtgggcca tgactacctg ctcctcacga ccgccagcag cgccatcggc ctgtcgacca
     1021 ccgtcggcct cggccagggc cgcttcgagc tgagcggcaa ggcgggccag gaggtcgcca
     1081 tcaacggcaa caacaaccgt ggcctgttcg gcgatccgct cgccgagggc tcgagccggg
     1141 cagccgcgcc gccggaggga ccgctgccct acaccttcct ggcgaacacc attcgcggac
     1201 tcgtcggctg agcacggagg acatcatgaa gagaaccatg ttccgtagct tcgtgttcgc
     1261 cgccgccacc gctctgctct cccaggcggc cttcgccgcc ggcccgctcg agatctgcaa
     1321 ccccggccag ccctatcgct gggccgccgg cggcgtcaac atcccgtgga acccggacca
     1381 gggcggcctc ggaccgctga ccaacgccca ggcgatcgac ctcgtcgccg acgccttcca
     1441 gggctggggc gacatcccga cggccacgac cacctatctt cagggggcgg cgctggcggt
     1501 cgacgtcgat gacacgaact tcgtccccta cttcttctcc gacgccccgg acggcgtctc
     1561 ggccatcgtc ttcgacgagg acggcgcgat cttcgacctc ctcttcggcc ccggctccgg
     1621 cgtcctcggc atcgccggcc ccgagtgggg tgacatcccc acctgcacca tcaccgaggg
     1681 cttcgccttc ctcaacggcg gcgccttcgg cgacccggtc gaggccttcg acgtcacctt
     1741 ccacgagttc ggccactacc acaacctggc gcactcggtg gtcaacgggc agatcgccgc
     1801 gctcggcgac tactccggcc cgacgcccca tgacaccttc cccatcggct cgctggtgaa
     1861 cctgatcgag acgatgtacc ccttctatct gggccccccc gccggcatgg gcacgccgca
     1921 ccgggatgac atcgccagcc tgtcgcagct ctatcccacg gcgagcttcg ccagcaccac
     1981 cggcaccatc accggccgga tcttgggccc gaacggtacg acgcagctca ccggcgtcaa
     2041 cgtcatcgcc cgcaacgtcg ccgacccgtt cgccgacgcg gtgtcggcga tctcgagcga
     2101 cttcaccgac acctataccc agggcgcgcc gggggtgggc gtctacaccc tgcgcggcct
     2161 gacgccggga gcccagtacg ccgtctacgt cgacgagatc ctcgccggcg gcttcagcac
     2221 gccgcccctg ttcccgctgc ccgggcccga ggagctctac aacggcgcga gcgagtcgtc
     2281 caacggggcg accgacaatc cgagcgcctt cacgggcgtc gccgcggcgg ccggggcgac
     2341 ggcgagcaac atcaacatca tcttcaacgc cccggcgccc aacacgccgc tgccggtggg
     2401 agatgacagc tccgtggagc tcttcctgcc gttcaccttc gagatctgcg gccagccgtt
     2461 caactccgtc tacgtcaacg gcaacggcaa cctgacgttc ggtgtcccca atgaagactt
     2521 cagcgagtcg atcgacgagt tcctggcggg cccgccgcgc gttgccggcc tgtgggacga
     2581 cctgaacctc gacggcggcg gctcggtctt cttccagcag agctcgaaca ccttcaccgt
     2641 cacctaccag aacgtgccgg agtacccagc caccggcgcc aacaccttct cgatccagct
     2701 caagcgctcg tcgaaccata tcgacatcaa ctacggcacg ctgaccgcga ccgacggcct
     2761 cgccggtgtg agctgcggcg gcgccatcac ctcgggcttc gagcgggcca ccaacctcac
     2821 cagtgacaac ggcaccaacg acctcaagaa gcagcccgcc atcttcgagc tgttcggacc
     2881 gggcaacctc aacgacctca gcggcaggtc ggagaagttc aacgccaaga cccgcttcgc
     2941 cgacgagttc gagcacaacg acacgctggc ccacgcggag gacgtcaggc tgcccttcca
     3001 caccgaggac gtcgacgact tcaccgagat ctctccggaa ggcgatgacg tcgacttctt
     3061 cgagttcagg gtgaaggagg gggacatcct cgcgatcgag gtggtccgcg gcaacctcga
     3121 cacggtgctc ggcctcttcg acgccgacac cggcgacctg ctcctcgccg acgacgacgg
     3181 cggcaacggc ctgctgtcgc ggctgctggt gcaggtgccc gccggcttcg acgagctcgc
     3241 cgtggcggtc tcgacctacc ccgacgtcga cttcaccggc gccggcgagg gcagcggccg
     3301 ctacgtgctg cagatcacca agtacgaggg caccgtcctc gccgccggtg acgacacgtc
     3361 caccccgctc ccgctcggct tcaccttccc ctaccagggg ggccagtaca ccagcgtctt
     3421 cgtcaactcc aacggcaacc tgaccttcaa cgcgggggac gccgacctct ccgagacggt
     3481 gccggagttc ctcgccggcg ctccgcgcat cgctccgcgg tgggacgacc tcgacgccac
     3541 cggcggtgtg atcgtcgcct cgccggacga cgatgacaag atgacgatcc acttcgtcag
     3601 cgtgccggag ttcttctcca cctctcccaa ctacttctcc gtcgagctcg acgaggacgg
     3661 cgacatcgag ttcgagtacc tcgccaactc gcgcaccgac ggcctcaccg gcatcaccaa
     3721 gggcggcggc gtgcccgatc ccggagagga caacctctcc cgggacgacg agcaccccgc
     3781 caccggcacc acctacgagc tcttctcgac cgccgacgtc ttcagcttcg acctcttcta
     3841 cagcgacgtg aagttcgacg aagacgatga cgacgacgat gacgacgacg acgatgacca
     3901 cgatgacgat gacgatgatg atgatgacga ctagtcgtcg cctcccctga cgggaccttc
     3961 caggcggcgg gcgcgagccc gccgccttct tccttcaggc cgtctggacg ttcctccccg
     4021 ctcatccgta tacatctatg agggccggaa gacctccggc cgaagcgaca gaaagaacga
     4081 caatctccca gcgggggctg ccgtcgcggt ctccgccctt ttttgtccct tccctggaaa
     4141 cctccactcc tcgcggccgt atcagcccca tgacccgagc aaccagagcg agcgccagcc
     4201 tccgagtccc gttcatgccc atcctcgcct tcctgatcgc ctgcggcacc ggcccggcgg
     4261 ccacggcccc ggccgacaca cggccgttcg actacgattc caaggctccc ctggatacac
     4321 gggtcgtctc caccgagtcc cgcgacgggc tcaccatcca ggagatcgag tacgccagtc
     4381 cccgcggcgg ccgggtaccg gccactgtca tgatcccggc cggaaagggc cccttcgccg
     4441 gcatcctcct gatgcatgga gctccgggca gccgccggca gaccttcaag gaggcggagg
     4501 agttggcgcg cgcgggggcg gtcaccctgt ccatcgacgc ccccttcgcc cggcagggcc
     4561 gcccggccgg cgagatcctc cgcttcgacg agcaggaccg ggaggactcc atccagctca
     4621 tcgtcgacct ccgccggggc gtggatgtcc tactggcccg ctcggacgtg gacccgaagc
     4681 gcctcggcta cgtgggcggc agctacggcg gcgccatggg cggactgctc gccggggtcg
     4741 agaagcggat caaggcctac gcgctgttcg tgggggacgg cggactggtc agccatttca
     4801 ccggcgcgga cgacaccaac ggaccgttgc agcagatgcc gaaggatcgg gcggaccgct
     4861 ggctcgcggc catgctgccc atcgagccga tccacttcgt gggtcgcgcc gcgccggccc
     4921 acctcctctt ccagaacggc cggcaggaca ccatggtgcc ccccgccgac gggcgcgcct
     4981 tccaggaggc cggcagcgag ccgaagacta tccagtggta cgactccggc cacaagctgc
     5041 cggacgaagc ccgcgccgcc cggcgcctct ggctcgcgga acggctggga ttgaagggtt
     5101 gacgggctga aggaaaagga gggagcggcg cggggccgct ccctctcgat gggtcgcttc
     5161 aggccctacg gcgtgggctt gcgggacagg accagcgaga accggtagtt gaggtcgggc
     5221 tcctgggtga acgtgacgcc gtcggtggag ctgtaggagt agccgtagat cgggccgtcg
     5281 tcgccgcggt cggtgccgac cagcggcgga atccccgcga ctccagggaa gggagtcgtc
     5341 gtcggcagcc gcagaaccag gaagaggtcc ttgatcgtcc cgtgctggat gccgttctgg
     5401 acgatcgagc cgagcgccac cggcgactgg aagtaggccg gggacaggtc gttgtcctgc
     5461 ccgacgaagc tcatctcccg cgcgagcggg tgggcgaggt cgatggtcgc ggtggaaccg
     5521 ttcaccgtgc ccgtcgccaa catcgcctcg gcgaagaccg ggacggtgga ggagtccacg
     5581 accagggtgt cgaactgcat ggcgtggaac agcacgtcct cccccgcagg caccgcgccc
     5641 gtgatctgtc ccgcgggcac ccggaccgcg tagtagagac cggcgggcgc attgccgctg
     5701 acgaagttct ccacgtcacc gaagttggcg atctggatgt cccggttggt gatgaagtcg
     5761 actcccgtcc gcggcaggcc cggcacggcg acctgcggaa tcgcctggcc cgagcgcagc
     5821 tcgagggcgc cttcgccagg accgttgaaa aactcctcat tgaagctctg ctggccgaag
     5881 accgcgccga tcaaggccgt ggtgttgaag gatccggcgc cgacggggcg gccgtccagt
     5941 ggctcgatcc cgatgttcca gactcccttg cggaccggca ggacatagtt gccgtccagg
     6001 atgttgaacg agggggagag gatgaattct tcgccggtga tctcatccac cgaaacctgc
     6061 gtggtaccgc tgaacgtgct ggagaagatt ttttgctctc cctgggtggc gctcgcgcgg
     6121 accaccgccc ccgccaccgg ctggctgagc ccgccgtgcg tgacgctccc ctggatcagg
     6181 ccgtaggcct ggtggaacgc aacgtctccc ggctggagcg cggccagtcc ggacgccgcc
     6241 gtcccttcgg gataggtata ggagctccag gcgatgtcgt cctcttcgag cgtgcgcgcc
     6301 gccaactcgt gggccgggag agtagggttg acgaacggaa acatggtggc tccatcgccg
     6361 tccgtgtcgc tcacctgatc gatcacggag tgggagagcc cgtgcgagtg gccgaactcg
     6421 tggacggcga cggcctcgaa gtccaacgac cgctgaacgg tgtccgcctc gtcatcgttg
     6481 atggtgaagc gatagccgcg ggcggcggtg gtgttgaact cgacgtcgtt ttcaaagatc
     6541 gtcccggcct cgtagaagcc cgccgggaac tcgatgtcgc cgtcgttgtc cacgtcggcg
     6601 caggtggcga tggtagcggc aacgtcggag tcgccgtcct cgtcgatgtc gtcgccgggg
     6661 aacagggtga gatcatagat cagggagtac ggcgaggaaa tggcgatggc gccgaaggcc
     6721 ggtgtacgga acgtcacctc gttgatccca tcaaatgcgg cgaacccggg attgggcacg
     6781 gtaccgacga cccgcatgtc gatgaacgag gtggggatcc ggttccacga atcgaaagct
     6841 ttctggatca ccggcgtgac cgccgctacg ctcaggaacg gcgtgccgag aggattgggg
     6901 atcggattca gggtatcgtt cactcggaag tggaccggga tgcagcgcga gtcccagtgg
     6961 accgggacgt tcttgccgat gatgtgaccg gggatgggag acgggacgaa gcctgtgacg
     7021 tcatagacct cgatgttgcc gccggcgaag accggggcgg cgacggacaa cgtggccagg
     7081 accacggcca ggccggttct cacgaaaacc tttctcatgg ctcgctcctc cgtccggcca
     7141 cggcgtccag gatctgccgc cggaattgat cataggggat cgcccgcgag ttgccgtgcc
     7201 cgaggccgcc cgacttgccg accaggctga gcccgcccag gtcctgggcc gccatcttct
     7261 tgccgtcggg cccggtcacc agggtgaact tcccctgcga gtagcccacc accatgtagc
     7321 cgtccggcac ccgcgtctcg ggtgtgagga agagcagcgc ctgctcctga tcacccaact
     7381 ggggcgccgc cgggtaggtc ttcgcgatgg gaacgcgccg cttcaggtcc acgcctccgg
     7441 gcagcaccac cgtgatctga gtgcccgcct ccgccccctt gaacccttcc gagacggaga
     7501 acgtcgccag cgtcaccaga tcgcgcccga cccaggcgct ctccttgtgc acacaatggc
     7561 ccgcgacgat gacctgtgcc tgccgcatca tgtcctcggg ggtcacctcg acgaccgtcg
     7621 tcgccgatga gactgccggc aaggcggcca ggacgaaggc cgtcccggcc aaggcgttca
     7681 acaccttttg catgaccaga tcctcctcgg tcgagcgtac ccgccatctc gggaagctca
     7741 atgaattaga caggtaggat tgaagaaagt tcccgtggat cttgaagaaa gatttatgag
     7801 tacgagccga acggaatgaa gtcggctcgc cgaagaaccc gtgggccggc ttcgtttccc
     7861 gaggcgcgac ttcaaacgcg gcggcgcgac ttcatttgcc gtggctcccg ggaaacgaag
     7921 ccgacccaaa ccgaatgaag tcggcccgct ggaaaatcca tgagccgact tcgtttacca
     7981 gggcacgact tcaaacgcgg cggcgcggct tcgtttgcgg cagctcccgg gaaatgaagt
     8041 cgggccagac aaactcaagc cggcccgctg aaaacctcgt gggccgactt gatttccagt
     8101 gaagcgactt cattcgatgc cggccagccg gaagtgacaa ggctcggagc ctctcccctc
     8161 tcacttcccc tggcgccgca gctccatctc gaagaactgg gtccactgcc cctggcgctc
     8221 gatgtggccg acctcgtgcc agcggtcccc ttcaatgcgg atggtgtagc gggtgcggcc
     8281 gccgggggtc tccatctccc agatgaacgc tccgtcctcc acgcgtcccg ggaagtcgcc
     8341 gctacggccg ttgccgagat gggtgctgaa ccggtaaccc ttcttggccg gatcgtacgt
     8401 cagcaccgcg aaggcgtgat ggacgacgcg cgagcggtcc ggcgtccagt gcctgccttc
     8461 gacgagaagc acgcggccgt cgagacgggc ctcgaccgtc tcctccccca cgaagcgctc
     8521 cggctctccc ggccccatcc gcatccagcc gccgccctcc cagctcccct gcatcatggc
     8581 gacggccttc atcgcctcca gaccgggatc gggcgcgccc gccggcgatt cggccgcacc
     8641 ggccgcgatg aacgacgcca tcatcagcga cagcgcgaga atcaggttct tcctcatgtc
     8701 agtctcctcc cttctggccc acagtctcgc tccccgggcc ctgccgcgtc ttgacgaaaa
     8761 cggtcattcc gggagcagcc ggtggtcgat gcgccgagcg tgctgctcga accggccggg
     8821 ggtcaccccg aggaggctac ggaactcgcg taccaggtgc gcctggtcgg cgaagccacc
     8881 ctcggccgag acgtccgccc agggccgcac ccccagaacg gccgaagccg cggaggaccg
     8941 caaccggagc acccgcgcca gctccttggg cgagagcccc acggcggcct ggaagcggcg
     9001 gcggagctga cgcggtgaca gcctcacctc tgccgccagc tcttccacac gggttccctg
     9061 atcggcctgc tccgtccgca gcgaacggcg gaggctgtcc acggccgacc tcaccaccgg
     9121 atcgggtggc tcggcccgcg gcatgagctc gcggaggccc cgcgccaggg cgtcgagggc
     9181 ggccatgtcg tcctccccgc ccgccaccgg cccccagctc ccgcaccagg cccggtcgag
     9241 ccagagatgg gccggtccca tcgcgtcccg cagggagcct ggcgggaggt ggaggaacgg
     9301 gcgcgcgcct ccgggatgga agtggacccc gcggaagagg tcgccgcccc ggacggtcgt
     9361 gaccagcggc gccgtccgcg gcccggccag catcggcagt ccggagcggt ccaccgacag
     9421 aagaagaccg ccggtgagcg ggatccggtg ctcgatctcc cccgcgccgg cccggacgcc
     9481 gaagcaccag aaggccgcca cccacgggtg gagctccgcc ggcggcggga tctccacata
     9541 gctcatcgga gctggatcca taaggatgac gataccgtag gctctccagc tccgcgtccg
     9601 caagctggaa gtcgcttaga gccctaccgg accgtttccc agagatccgc caaggcgtcg
     9661 tccagggttc ggcgcggctc ccagcccagg gcgcggaggc gcgaggggtc tcccgccaag
     9721 agggggatgt ccaccgggcg gatgcgctcg gggtcggtct cgaggcgcgc ctggacgccc
     9781 gagacggcca tcaggcgctc cagggcctcg ccgatggaga cggccttgcc ggtggagatg
     9841 ttgtagatcc cgcccggctc ccccttctcc gccagcagac gcaaggcggc gacgccgtcg
     9901 tccacgtggg cgaagtccct ccgggccgag aggttcccca cccgcagcac cggctcctgc
     9961 tcgccacggg cgccccggga gatagcggca agctgcatcg cgaaggcggg gagcgcgaag
    10021 atcggggact gccccggccc caccaggttg aacgagcgga ccaccacggc gcccgcggcc
    10081 agcgccagac gctccgccgc cgccttggtg agggcgtacg gcgaccgcgg gtccagcccc
    10141 tcgtcctcgc cgatgggctg ccgctcgtcg gggacgatgc cgtagacctc ggcgctcgag
    10201 gcgaccagca cccgccgtcc gccccggcgg ccaaccgccg ccagcaggtt ctccgtcccc
    10261 aggacattga cccggaagta gtccgggatc cgcttccagg actcccccac gtgcgagagg
    10321 gccccgaggt ggaccaccac gtcgggcgcg gactcgtcca ccgcccgccg cagggccgcg
    10381 gtgtcgagca ggtccgcctc gaagaggtcg accccgtcga agtcggggcg cgtttcgaga
    10441 aaggtgccgc tcacccggtc cccctgcgcc aggaggtgcc gggcgagccg cgtgccgaga
    10501 aagccgccga tcccggtgat caggaagtgc atggagatcc agcccgcgcc ggcagccgaa
    10561 cgggccgggt cagctccgct tccggtgggc gagggtctcg cgccagtagt ccaggaggtc
    10621 gcgcaccgtc tggtcgaacg ggatgcgcgg ctcccagccc gtgtcctccc ggaacttgga
    10681 gctgtcgccg atcaggatct cgacgtccga cgggcgcagg cggtcggggt cgacctgcac
    10741 gtccaccttg acgttcgaca gcgccagcac cttgtccagc aggtcgcgga tgctgatgcc
    10801 ggagccggtg gcgatgttgt agacctcgcc cggcttcgcc ttggtgaccg ccagccagta
    10861 ggcccgcacc atgtcgcgca cgtcggtgaa gtcgcggatg gcgtcgatgt tgcccacccg
    10921 gatcaccggc tcctggagcc ccagctcgat cgacgccacc tggctgcaga agttggaggt
    10981 cacgaacacc tgcccgcgcc gcggcccggt gtggttgaag ccgcgggtgc ggatcacctt
    11041 gatgccgtag ctctggaagt actggtagcc gaggtagtcc tgcgccacct tggagacggc
    11101 gtagggggag agcggccgca gcgggttcgt ctccttgatc ggcacctcgt cggggagcac
    11161 caggccgtac tgctcggacg agcaggcgat ctggatcacc ggatcgagct tcagcgcccg
    11221 caccgcctcg aacaggttgg tctgcccgga gacgttggtg accagcgtgt cgttcggcgc
    11281 cttccagctc gacggcacga agctctgcgc cgccaggtgg aagatgtaat ccggccgcga
    11341 ttgctcgagc gccgcgtgca tggacgtgta gtcccgcagg tcggcctcca gcagcgccac
    11401 cttggagcgc aggtgctcca cgttctccat gcggctgcgc cagcgatagg tgccgaacac
    11461 ctccacatcg gggtgctcgg ccagcagata ctccgccaag tgcgatccgg cgaagcccgt
    11521 aattccagtg atcagagccc tcatcaggtc ctcccttgtc tcatggcagc gatgtactcg
    11581 accaggcccc actcccatgg ctcgacacgc cgccccacga gatgctcgaa gcgggagacg
    11641 tcgaggacgg agtaggccgg gcgcggggcg ggccggggaa actcggccgt ggtgaccggc
    11701 accacgtcgg cgggacccga ggggccccgc cacaggcggg cgatctccac cgtgaaatcg
    11761 taccaggtgg cggcctcgcg gttgcgatag tgcaccactc cggtggcgga cagcggcgcc
    11821 aggtccagca acgcctgggc caggaacggc gtataggtcg ggcagccgcg ctggtcgtcg
    11881 accacccgca gcggcagccg cccctcgttc atcagccgga tcatggtgcg gacgaagttg
    11941 ggtccgcccg ggccgaacag ccagctggtg cggacgacga gcgcgtcagg atgctccagc
    12001 gccctcgtct ccccttcgag cttgctccgg ccgtagaccg acatcggcgc gaccggcgcg
    12061 tcctcgcggt agggctcgcg gccggcgccg tcgaacacat agtcggtgga gacgtggagc
    12121 aggcgcgccc cggcccgccc ggcggcggcg gcgacgttcc ccaccccttc gccgttgatg
    12181 gcgtaggcgc gctccggctc ggtctcacag acgtccacct gggtgaaagc ggcacagttg
    12241 atcaccagct cggggttgaa cgtgtcggcc cagtaggcga ggcggccgcg gtccgtcacg
    12301 tccgcctgga cgtgcgacag ggcgagggcc gcccagccgc gggcacgggc ctcccgcacc
    12361 acggcccggc ccagcatgcc ggtcccgccg aagatcagag cgcgcaagcc gttccttcca
    12421 cccgggtcag gtgatcccca cctcggagtt gtcgcccagc atgaagcggt aggccttggg
    12481 tttgaagtcg gtcttggtga tgcggacgtt gcggccgatc agcgagccgt cgatgcgcag
    12541 cgggatgtcg cggatctcgg aatcggccag gatgatggag ttctccacct cgcagctcac
    12601 cagcgtgcaa cgctcgccga tcgaggtgta ggggccgacg aaggcgtgct ccaggcgcgc
    12661 gccgtcgccg atcaccaccg gcccgcgcac cagtgagtcc accaggacga ccccttcgcc
    12721 gatctccacc cgcccctcga tgcggctgcg ctcgccgatg tccccgaggg tgacgccgcg
    12781 ccgggcgtcg aggctctcga ggacgatgcg gttggcctcc agcatgtcgt cgagcttgcc
    12841 ggtgtccttc caccagccgc ggacgatgtg ggggtggacc gccaggcccc ggtcgatgag
    12901 gtcctggatg gcgtcggtga tctccagctc gccgcgggcc gacgggcgga tccgcttcac
    12961 cgactcgaag atcgtggagt cgaacatgta gacgccgacc agcgccaggt tgctcttcgg
    13021 ctccttgggc ttctccacca ggcggtggac cttcccctcg ggggtgagct cggcgacgcc
    13081 gaactgctcc ggcgtgggca cctcggcgag gaggatctcg gagttgcagt cctgcgaccg
    13141 gaactcgtcg accagctcgg tgatgccgcc ggcgatcagg ttgtccccca ggtacatcac
    13201 gaaggacgaa tcgcccagga agtcttcggc gatcatcacc gcgtgcgcca ggccgcgggg
    13261 ggcgtcctgc tcgatgtagg tgacgcgggc gccgaagcgg gagccgtcgc cgacggcggc
    13321 gcggatctcc tcgcgggtgt cgcccacgat gatgccgatc tcggtgatcc ccgccgccac
    13381 gatcgcctcg atggcgtaga agagcaccgg cttgttggcc acgggtacga gctgcttggc
    13441 cgaggtgtag gtcagggggc gcaggcgggt gcccttgccc ccggacagga tcagaccttt
    13501 catcgaatct ccacgcactc gaaatggaca ggacggcggc tacggttcct gcgacgtgct
    13561 gctgcggctg gtgaggtcga ggaacgtgcc gacgttcttg aggctcaggg aaaaacggaa
    13621 atccttgtca cggatccggt cgccggcgcc ggcccggaag tcgcgcatct ccacccggac
    13681 accgtagcac tgcccgctcc agctcagcgc gtagatctgc tgctgcagga gctgggtcgt
    13741 gaggctgtag ttgatctggc tgtcgaggcg cagcttgttg ggcaggagct cgaggccgcc
    13801 ggtcaggcgc acctggttgt ccagctcctc gccggtccgc ggctggaaac gggtgaacca
    13861 ggtgaggccg atggtgtcgt tcttccggaa gccgtagttg cccgacagcg aggtgctgga
    13921 gaggcccgag aagagcatgc tgtagtcggc ctccagcttg acgctggtgc ggtcggacgg
    13981 gttgaagcgc agcagcgcct cgagcggtcc ggcctgggcg gtctggaacg aggtggggtc
    14041 gtcggggatg gtgagcccct gctcgagcgg ctgctcggag tcgaagctgt agcgccgcga
    14101 cacctcgaag tagaggacct cgcgggcgct cacgttcacc ggcctgacgg gcgccgccgg
    14161 cggcgccggc tgccccggca caacgggctg cgccggtggc gcgggaggtg ccggcacctc
    14221 ccgcggcttg cccaggatgc ggttggcgaa gacgaagcgg ccggagttgg tggagcgcag
    14281 ggtgtccacc tcgtcgaaca gcggcacctc gttctcctcc tcgaactcgc cgaggtagtt
    14341 gtaggtccag cgcggctcga cgacgtgctt gaagcggccg aagctcccca ggttcaggtc
    14401 gaagatgcgg gagaacgagg ggcccacgat ctcggcggta cccgagggga gggcgcgcag
    14461 caggctctcg ccgctcagct ggctccggtt gtccagatcg acggtgtcct ggtagtaggt
    14521 gacgcgcccg ccgccggtca gcgtcaggga gagccagggg aaggagcgca ccggcaggct
    14581 gagctgcggg aagaggtcga ggcgcccgta gctgcccgag aagccgttcg gccggtcgat
    14641 gtccaggtag gacgccgagc cgtcgaactg catgtagagc ggggtgcggc cgatgcgcgt
    14701 ccggcgcagg gtgtagtcga gctccggcag ccgccgctcg tcgagcgttt cgttgctggc
    14761 gttgacgaag gtctgccggt cgttgagcag cagggtgaac aggtgggctc cccagttgcc
    14821 ggtgacgaag gcgcggctgt cgatgaagcg ccgggtgttg cggtcgaagt cgcgctcgaa
    14881 gtcctggaag aagttgaagt ccgagtagtc ctggaaggcc accacggcgc gcatgccgcg
    14941 ccacaggtcg cgcgtctcgt ggttccactc gagcttccag cgccactcct cgtcggtcac
    15001 cgactccggg tcgcggatca catagccgag gaggttgccg cgcgtccctt cgctgggacg
    15061 gtagcggaac tcgttgccca ggccgagata gctctcggtg taggtgtcga cgtgaaaggt
    15121 ggtgtcgtag ctccgcccca gggtctggaa gtaggcgagg cccaccgatg cgccgcggcg
    15181 gtccgagtag ccgatgttgg ggatcaggaa gccggagctg cggtcgggct tcaccggcca
    15241 caggatgtag ggggtgtaga agaccggcag cttcttggcc cgcatggagg cgttgcggac
    15301 gtgggcgtac tggtcctcct cgacccgcgc cgagccgagc cgggagctcc agtccggcac
    15361 ctcctggctg caggaggtga agacgccgtc ctcgacggtg tagacggtgt cggagatctt
    15421 cgccacctcg ctgccgctga agtagtagtc ctgcgagacg ttggcggtgg cgttggtcag
    15481 ccggccggtc ttggtgtcga ggtcgtagtt ggcggtatcg ccggagagcc gccgcggccc
    15541 ctggtcgatg acgacgttgc cgcgggcggt gacgatcttg gtattgaggt cgatctccgc
    15601 ctgctcggcc tggaggttga cgtcctggta gcggatgcgg acgccgccgg agagcacggc
    15661 gtagtcgtcg cgctggtact cgagcgagcc ggcgctgccg gcggcgctgc cgccaccctg
    15721 gtccgccgga aagttgaggt tgaactcgat gcggtccggc ccggcgcccg gcaccgccgg
    15781 cacggcgggc gggacgatgc ccccgccctg cggctccggc agcgtctgcg gcggcatggg
    15841 cggcaccgac ggcgagtccg gctgctgctc ggacgtcggc ggcgtcaccg gcgtcgccgg
    15901 aggggtcgcc gccggatccg tgggcggagc cataggctga gccgtctgct ccgccggctg
    15961 ggccggagcc gtggtctggg ccgttgcctg ggcgggcagg ggctgggcct gcggctgggc
    16021 aggctcctgc cccgacgccg gcagggtcag gcagagggcg gcgatcagga gaccggtgag
    16081 ggggcggcgc gtatcgagca tgagcgccgt ctagtctaag cgctccacca ccatggcggt
    16141 agcctcgccg ccgccgatgc agatcgcggc gcagcccaga cggccgccgc gctcccgcag
    16201 ggcgtgcagc agagtcacca ggatccgcgc ccccgaggcg ccgatggggt gccccaacgc
    16261 caccgcgccg ccgcggacgt tgacccgctc ggcgtcgagg ccgaggtcgc ggatgaaggc
    16321 cagggccacc accgcgaacg cctcgttgat ctcccagagg tcgatgtcgg ccgccttgag
    16381 cccggcgcgc tccagcaccg accgggcggc gccgaccggc gcggtggtga accactccgg
    16441 cttctgggcc agcgacgagt gggcgaggat gcgggccagg gggcgggcgc cgatgcgcgc
    16501 cgcgtgcgac gcggtggtca gcaccagggc ggcggcgccg tcgttgatct tgctggcgtt
    16561 gccggcggtc accgtgccgt ccttctcctg gaaggccggg cgcagcgagc ccatgcgcgc
    16621 gaggtcgacc ttgaacggct cctcgtcgcg gtccaccacc ttggggtccc ccttcttctg
    16681 ggggagcgag accggcacga tctcctcggc gaagagcccc ttctcggtcg cctcgcggga
    16741 gcgccggtag ctctccaggg agaaggcgtc ctgctcctcg cggccgagct ggtactcgcg
    16801 ggcgcagagc tcggcgcagt tgcccatgtg gaggtcgttg taggggtccc acagcccgtc
    16861 gtggatggtg gagtcgatca gccggccgtg gcccaggcgc aggccgtcgc gcacgccgtg
    16921 agcgaggtag ggggcctggc tcatgctctc catgccgccg gcggcgacga gctggaagtc
    16981 gccgcagcgc aggtcgttgg cggcgatcat gacggagcgc atggccgagc cgcaaacctt
    17041 gttcagggtg agggcgccgg tgtgcggcgg gcagccggcg ccgagggcgg cctggcgggc
    17101 cggcgcctgg ccctggccgg cggtgaggac gcaacccatg ttgacctgct ggatatcctg
    17161 gggggtgacc ccggcgcgct cgatggcggc ggcaatggcg cggctcccca gggcgtgggc
    17221 cggcaggccg gccagggcgc cctggaacgc cccgatgggc gtacgaaccg cgctcagaat
    17281 gacgacgtcg tccatgaaac ctctcctgtg gagcgtgatg cctgcgggtc gtcccgccgg
    17341 gcgggcaaac gaagcacccg atgctagcac atcccttccg gaggcccggg aagctacaat
    17401 gcgtcgcgga atgagcgccc gggccctcgg catcgacttc ggcgagaagc ggatcggcct
    17461 cgcgatctcc gatccggagg gccggctggc cgtgcccctc accacgctgg agcggcgcaa
    17521 ggaccgggcg gcggtggccc agatcgccga gatcgcccgg cgcgaggggg tggggcggct
    17581 ggtcctggga gagcccgtgg gcctcgacgg ccggcgcggc gaggcggccg agcgggtccg
    17641 gcgcttcggc gagaagctcg ccgccgccgc cggcctgccg ctcgagatgg tgaacgaggc
    17701 gctcaccacc gtggaggcgg cggagcgcct gagggaggcg ggggtcgacc cgcggcggga
    17761 gccggagcgc ctggacgcga tcgccgccca gatcctgctg caggaggcgc tcgatcgcag
    17821 ggggtgaccc cttgatggga ggaacggctt ggcacggaac accacggcgg gaaagggcaa
    17881 ggcaaaggcg ggcgccaagt ggagcggctt caagcgcttc ctgaccggcg ccggcatcct
    17941 gctgctcctc ctcatgatcc ccgtcggcat ggccgcctgg tcgtggttga aggtgcagcg
    18001 cccctataaa ggctacgagg gggcggagaa gctggtcacc gtcgagcccg gcatgggggc
    18061 gagccaggtg ctcgacctcc tgcagcgcga gggggtcctc gccgacgcca agctcgcccg
    18121 cccctacctc atctatttca tgggcgaccc gcggatccag gcgggcgagt acctcttccg
    18181 cggccccttg agcacccccg aggtgctgcg caagctggcc aagggcgagg tcgccacccg
    18241 ctcggtcacc ctgatcgagg ggctcaccct cgaggaggcc gcccgccaga tcgccggcaa
    18301 ggggttcggc cgcgaggagg tgctgctcga cctcatgcgc tcgccggagc tgatcgccga
    18361 cctcgacccc gaggcgaccg acctcgaagg ctacctcttc cccgagacct acagcttctc
    18421 ccgcggcacc agcgaacggg aggtggtcgc gacgatggtc aagaccttcc ggcggctgta
    18481 cgaacgggac gtccggccgg tcctcgcacg caatccggac ggccgcaccc tgcgccaggt
    18541 ggtgaccctg gccagcatcg tcgagaagga ggctcaggtg cccaccgagc gcccgctcat
    18601 cgccggggtc taccgcaacc ggctgcagcg cggcatcggg ctcggcgccg acccgacggt
    18661 gatctacgcg ctcaaacggc tgggccgctg gaacggcaac atccgccgcg acgacctgcg
    18721 ggtcgactcg ccgtacaaca cctaccgcta cgccggcctc cccccgggcc ccatctgctc
    18781 gcccggcctg gccagcctgg aggccgccgc cgcccccgcc gacgtcccct acctctactt
    18841 cgtcagccgc aacgacggca cccacgtctt cgccgagacc ctcgccgagc acagccgcaa
    18901 cgtcgagcag tggcagcgcc gctactggcg cgagcgccgc gcccgcgagc ggcgcgagcg
    18961 cgaagcccag gcctccggcg ctaccggcag ccctggccgc cagtagccgg cgcgagcctt
    19021 cgcgcgagcc ctgtaaattc ttctggattg acagtttcgg ggcggtggtg tatctaggta
    19081 tacagattca tctgttttat ccacgcgcgc catcccctgg caggtccctg taggccttct
    19141 tggcggcgcg ccggcccggt gaaggaggct gcatgaatat tccggaggag ttcgggaatt
    19201 acctactgct gaagaagttg accgaggacc cgctgggaga gacgttccga gccggcaagg
    19261 tgggcaagga ggggatggag caggtcgtgc tgctccgcgt cttcaacggc aaggggatgg
    19321 atggcgaggc gctctggcag aaggtgtccg gccgcgccgc cgtccagcag gcgctgcgca
    19381 gccccaacat cggcagcggc gccgacctgg ggaaggtgcg gagctttccg tacgcggcgt
    19441 acgactacat ctcgggcaag aacctggcga cagtcctcgc ccaggccgat cgccagggct
    19501 cgccggtccc caccgaccac gcgctgctga tcgccgagcg cctcgccctg gccctggccg
    19561 cggccgccga gacccgggtg caggacgagc gggtgctgca cggcttcgtg gcgccgcacc
    19621 tggtgatgat ctccaacgag ggtgagacgc gcctggtcgg cttcgaggtg gctcccggcc
    19681 tgcgcgccct cacggcaggc ggctggaacg acgaggcgct caagccctat ctggcccccg
    19741 aggccctggc cggcgctccg atctccaagg cggacgacgt ctggtcgctc ggcgccatcc
    19801 tctatgagct gctcaccggc gaacgcctcc cggcgacagc gccggcgggc ggcttcggcg
    19861 cgctgatcga cggcgccatg ctcgccaacg acgggactcc cctcccggcg gcggtggcgg
    19921 ccctgctcaa gaagagcttg gcgccggccg accagcgagt gggcgacgcg gtgagctggc
    19981 acaaggcgct gtcgaagctg atgatcgacg gccactacag ccccaccacc ttcaacctcg
    20041 ccttcttcat gcacagcctc ttccgcgacg agatcgagcg ggagaaccgc gagatcgagg
    20101 ccgagcgccg gatggacgtg ccgcagaggg tggccgcgcc ggtgccggtg cccgccgccg
    20161 cgccggcgcc gatccaggac ttccgggagt ccacgggcgt gcgccaggga accctcccgg
    20221 gcgtccaggc ccccgtggcc gcgcaggcgg cgcgctccaa ggcgccgctc ttcatcggca
    20281 tcgcggcggc gaccctgatc gccgtcggcg ccggtgcctg ggtggtgctg tccggctccg
    20341 gcggcaagga gacccaggcg gcggccgccg cgaacacggt gccgagcgcg gtgccgatga
    20401 acgccgcagc gcagccggcg gtcatgccgg gagccatgga ccccgcggcg gccggaccgg
    20461 ccgctccggc gggcccgagc cccgaggaga tccaggccca gatccaggcc atgttcgagg
    20521 cgcgcagcca ggagatggag gccaagctca agacgcagta cgacgaccgg atcaagcagc
    20581 tccagcagca gctccaggac agcaagaggg cggcggccga gccggtccgg cagccggagc
    20641 ggccagccct cgtcactccg ccggagccgt cgcccacccc cgccgagccc aagccgaccc
    20701 tgcgtcagga gccggcggcc gccgcgccgg tgatcccgag ccccaacctc tccgccggat
    20761 cgcagcagcc ggccgccggc acccagcagg aggccgcccg caccaccgcc agccagccgc
    20821 cggcccccgt caccaccgct ccggcgacgc aggcgccggc gccccggcag gtccagaccg
    20881 gcgacctggt gtcggccggc agcccgggat cggtcgcccc caagatcagg gtgctgcccg
    20941 acccccgcta tccaacggcg gcgcagaaga tgaagcgctc cgccgaggtc gccgtgcgcg
    21001 tcctggtcga cgagtccggc cgggtgctcg acgccgagcg ggtcggcaac aaggccggca
    21061 tggggttcga cgaggcggcg gtggaggccg cccgccgcgc cagcttccag ccggccacca
    21121 aggacggcgt gcgggtcaag atgtggacca ccgtccgcat caagttcacc ccgtaggcgg
    21181 cagcgacagc ggcaccaaaa ggaaacgccc ggctcgcgcc gggcgttttc ttttccgact
    21241 gtcctgccga agcggaggga ttacaggtcg gtggccttga tgctggccac gagctgctgg
    21301 atgaccggct tcaggacctt gtcgaacatg cggtcgtcat ccacgccgcc gccgtagccg
    21361 ccgacatgga cccgggcggt gccctccgag ccccgggcct cgtcggccca gacgatctcg
    21421 cccgtagcgg tgtcgatgag ccgggcgttc atcgccgcca cgaactcctt cttgccgacc
    21481 gacacgccgg ggaggcgacg gaagccgggg gtgttggcgc ccttctcggt gacgccgtac
    21541 tccgtcaccg agccggtgag gatgtagttg acccccagga gcttgccggc cttgacggcg
    21601 gtggccggat ccacgtcgcc ggacaagctg aggttcttct cctgcatcag ggcgtcgagc
    21661 tgctcgcgct cgatgacgcg gaacttgccg ctcttgaaca gctcggtgac gaacacgtcc
    21721 tgcgccgcgc cggcgccgcc gtgataccac cactggttct cggccttgtt cttgaactcg
    21781 atcacggcga tccgcggctt gtcgctggcg gccgcgaaca ccgggacggc ggcggccacg
    21841 accagcgcgg cgcagagaga gagtgtgaga gcgcgcttca tttcggaatc tccttgcaac
    21901 gtttgggtcc ctccgcgggg actccggttt cttcacctgg aacgtacggt tggcgtcctg
    21961 accctcattt tactccagcc tggccgcggg ccggcataat ccccttcatg atcatcgtcg
    22021 ccgacggctc cgagctcctc tgcatcaccc agcccgacca tgcccacctc tcgggggagc
    22081 tgatggccct ctggcgcgcc ggcggcgtgc cggagcaccc gcgccgggac gacctggtct
    22141 tcgccgcccg cgagcacgac aacggctggc gggaggcgga cgcggcgccg cgctgggatg
    22201 ccgcccggga ccggccgcac gacttcgtca ccattccgcg ggaggaccgg atcgccatct
    22261 gggagcgggg cacggcgcgg ttcgccgggg cgcgccccta cgcctccctg ctcatcaccc
    22321 gccacgccct gaccctccac cgcgaccgcc ggggggacgc ggcctgggac gacttcctgg
    22381 gctccatgga cgagctggag agggccctgt tcgaggagac cggcgcctcg ccggaggagg
    22441 tggcggccga ctaccggctg atcgacctgg ccgacatcgt ctccctcgcc gcctgcaacc
    22501 gctggagcga gccgttcgag cgccacggcg tgcgcggctc cttcgcggac ggcacgatgc
    22561 gcctcgatcc tctccccctg gcgggcgcca ccaccttcac ggtcccctgc cggcgcatcc
    22621 cgcaccgctc ctacggcggc gacgtcgcgc tgggcgtcgc gctcgccacc gcccgctggc
    22681 aggagctgcg gctgcggatc gcgggggccg aaggctgagc cggcccccct cagctcggga
    22741 tcttgaggtc ttcgggccgg ccggcgccgg agagcacgat ctgctcgaac accatgcgat
    22801 agagcgccag caggtcggca aagacgcggg tgaacgcccg gtcggtggac tccgacagca
    22861 gcgggtggcc cttgaggaga ggcgggatgc agaaatgctc gtcccggagc tccggcggct
    22921 ccttggcctc gccggagcca tcgccgccga ggcgcagaag atcggacgcc cggcgatcca
    22981 ccagatagag ggtgtagaag aactcgaagt gcccgacctc cagcgtgcgc aggaccttct
    23041 ccagcgagtc cagcttgggg agctccttgc cgttctcgta tttggagagc tggctcttgc
    23101 cgatacccgc ctcccgtgcc acgcaggcct gactcttgcc acgcagctca cgcaacaggc
    23161 tcagcgtgcg ccccagattg acgaacatgc ccatgcagaa aatttaccgg ctggagcgga
    23221 ccggcgcaag cacaaatctt tctgccgaaa gcacttacgg acacagacag atagccggca
    23281 acggctactg atgactgttg aaggcaacgg aaggttgtcc cggttgcggc gaccctgcat
    23341 caagatgtcg gcgcaagcca caccttccgg gccacacatc atcgggatcg gctacaaatc
    23401 acgacggcca ggccacgcct ggaggcgtaa agacgcaccc gtggacccgg aaaccggctt
    23461 cagcgcttct tcttgcggcg ttgggaggtg gacggcgcgg gcgcctctcc cccctccggc
    23521 ctgccccctt ccgggacggg atccggccgg tgcagggcct tgggaacgac ctggaagagg
    23581 acgaccacct cgccgagctc caccgccccc tgggcgatcg ggcgggactg gctgaaggtg
    23641 gactggacga acgatcccac cctccccagg gcctgccgga tgtcctccgc ccaccggccc
    23701 tccccctcct ccacagcggg cccctccacc gcctggggag gcgctcccgc aggcccctgg
    23761 agcgccagcg cctcggcctc caccaggtca tagcgcctga agaactcgcg ccgccccacc
    23821 ccctgggact ggaggatctt cgccagcgtc gagagcgtcg cctcctggcg ggcgttctcg
    23881 atgcgcgaca gttgcccgcg cccgagcccc gcctccgcgc cgagctgctc cagcgtctta
    23941 cccttctcct tacgcagccg ccgcagcgtc tcaccgatca gatcgaacat ttgcacgcgc
    24001 gaaatctact cgcgaggtgg cgcgtttggc aagcataagt cctttgagct gagggactta
    24061 gaaccacaca accgttgcct cttacggcga gttgttttct ccccccacca tgtggggcaa
    24121 ctcttcggtc ctttgtgggc aagaaagacc acttgccgga accaaatctt tcgttccagg
    24181 ccagggcctt tccgcccgcg atggaccggc ggccatgcgc ccggcatgcg ctatgcacgc
    24241 ctgctcgttc atggcatgtc cacgctataa ctgccaatat tcttgacaaa atctggacaa
    24301 cgagagtaat gtctctggac atgaggatgg ctactacccc tttccgagac cgtgtgacag
    24361 tgatcggcgc cgggccgggc gggttggccg cggccatact tctggcgagc gcggggctgc
    24421 gcgtcaccgt gctcgagcgc ctcccccggg ccggcggaag gacttcgacc ctggaggccg
    24481 acggcttccg cttcgacctg ggaccgacgt tcttcctcta tccgcaggtc ctgcgcgaca
    24541 tcttcgccac cgccggctac gacctggacc gcgaggtccc catggtccgc ctggaccccc
    24601 agtaccggct ggtcttcggg gagggaggcg agctgctcgc gacgccggac ctggagcgga
    24661 tggagcggca ggtggcagcc ctcgccccgg ccgatgcgcc ggcgctcggc cgcttcctcg
    24721 ccgacaaccg ccgcaagttc gagcgcttcc ggccctgcct ggaggagccc ttcctcggct
    24781 gggcggacct gctgaacccg cggctcctca agctcctgcc cgacctcaag ccctggcgct
    24841 cgctggactc cgagctcggc tcctacttct ccgacccgcg cctgcggctg gccttctcct
    24901 tccagtccaa atacctgggc atgtcgccgt tccgctgccc cagcctgttc tcgatcctct
    24961 cctacctgga gtacgactac ggggtgttcc accccctggg cgggtgcggc gcggtgacgg
    25021 aggcgatggc gcgggtggcg cgggagatcg gagtcgacat ccgcctcgac gagccggtgc
    25081 gggagatcct gttcgagggc cggcgcgcgg tcggcgtccg caccgaccgc ggcgactacc
    25141 ggtcggacgg cctggtggtc aacgccgact tcgcccgcgc catgtcacgc ctggtgccgg
    25201 accacctgcg gcggcgctgg acggaccgcc gtctggagaa gaagaagttc tcctgctcga
    25261 ccttcatgct ctacctgggg atcgaggggg tctacgatca ggtggcgcac cacaacatct
    25321 acgtctccgc ggactatgaa cggaacctgg acgacatcga gaaccggcac
//
LOCUS       DASPJI010000638         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_608607, whole genome shotgun sequence.
ACCESSION   DASPJI010000638 DASPJI010000000
VERSION     DASPJI010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608607"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VEL74_17965"
     CDS             <1..349
                     /locus_tag="VEL74_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64470.1"
                     /translation="RRPDLFDAVIAMSGFYDLGPDYLHGYSDDNCYFNNPAWYLPNLE
                     GHNLHLLRTACRIHILTGQGAYEAPHASRQLSEILHRKGIPHWLDMWGHDVDHDWPWW
                     LKMLPHAIGHMGW"
     gene            412..645
                     /locus_tag="VEL74_17970"
     CDS             412..645
                     /locus_tag="VEL74_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017651857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64471.1"
                     /translation="MATLNIKNFPEPLYRKLQEQAERDHRSMAQEVIHLLEKATETSE
                     PVSLLELRGLGKECWAGVDAVEYIRRERDSWDS"
     gene            633..1088
                     /locus_tag="VEL74_17975"
     CDS             633..1088
                     /locus_tag="VEL74_17975"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYG64472.1"
                     /translation="MGLLKEIGGGPVGLDTSIFIYLIEENPRYLPLIEPAFLALDDGD
                     LRGATSSLTLLETIVMPLRRGNRTVVERYEVLLTHGSGLRLVDIDRRVLQAAAHLRAV
                     TSIKTPDALQLASSMLAGCPVFLTNDDRIPAVPGLRVLCLDDFLEGAPS"
     gene            1210..2112
                     /gene="cyoE"
                     /locus_tag="VEL74_17980"
     CDS             1210..2112
                     /gene="cyoE"
                     /locus_tag="VEL74_17980"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HYG64473.1"
                     /translation="MQRTEIAVEQHAVPVSRLSDLSEMVKPGITLMVVLTTGLGFLLA
                     QESGVAVVLLLHTLLGTGLISGGASVLNQVLERDVDALMRRTANRPLPAGRLDPDTAL
                     LFGVSLAVLGLVELVFFVNLLTALLGAAALAGYVFLYTPLKRVTSLATVVGAVPGAIP
                     PMMGWSAVRGELDLPAWVLFGILFFWQMPHFLAIAWLCREDYGNAGFPMLTVDDPDGR
                     RTARQAILYCAPLVPLSLLPSVLGLMGTVYFIGALALGLAYLGFAFGFARHRSTAGAR
                     GLMFSSLLYFPGVLLVMLLDRIVS"
BASE COUNT          319 a          813 c          726 g          401 t
ORIGIN      
        1 ccggcgcccc gacctgttcg acgcggtcat cgccatgagc ggcttctacg acctggggcc
       61 ggactacctc cacggctact cggacgacaa ctgctacttc aacaacccgg cctggtacct
      121 gccgaacctg gaagggcaca acctgcacct gctgcgcacg gcctgccgca tccacatcct
      181 caccggccag ggggcctatg aggcgcccca cgcctcgcgg caactgtcgg agatcctcca
      241 ccgcaagggc atcccgcact ggctcgacat gtggggccac gacgtggacc acgactggcc
      301 ctggtggctc aagatgctcc cccacgccat cggccacatg gggtggtgac gtaggacacc
      361 ttgaaagtgg tcctccattc ctccagaatg gttccgtatg gaggcaaggc catggcgact
      421 ctcaatatca agaactttcc cgagccgctc taccggaagc tccaggagca ggcggagcgg
      481 gaccaccggt cgatggcgca ggaggtgatc caccttctgg agaaggctac ggagacctcg
      541 gagcccgtat cgctgctgga gctgcgtggg ctcggcaagg agtgctgggc cggcgtggac
      601 gctgtcgagt acatccggag agaacgagac tcttgggact cctgaaggag atcggaggcg
      661 gccccgtagg gctcgacacg tccatcttca tctacctcat cgaggagaac ccccgctatc
      721 tgcccctcat cgagcctgcc ttcctggctc tagatgacgg agatctcaga ggggcgacct
      781 cgtccctgac gctcctcgag accatcgtaa tgcccctccg ccgggggaac aggactgtcg
      841 tggagcggta cgaggtcctg ttgacgcatg gctcgggtct gcgcctggtc gacatcgacc
      901 gccgcgtgct gcaagccgcg gcgcacctgc gggccgtaac gtcgatcaag acgcccgacg
      961 cgcttcagct cgcgtcctcg atgctcgccg gttgcccggt cttcctgacg aacgatgaca
     1021 ggatccccgc ggtccccggg ctgcgggtcc tctgcctcga cgacttcctg gagggcgccc
     1081 cgtcctgacg ccagacactg ccggacggca tgccaccccc gggcgtgcat cctccgccgg
     1141 ttcgcgttag acttccaggg gtcccgcctc gccgcggagg ccccctgagt cttctgcgag
     1201 tctccaagga tgcaaagaac tgaaatcgcg gtcgagcagc acgcggtccc cgtgtcccgg
     1261 ctctccgacc tttccgagat ggtcaagccg gggatcaccc tgatggtggt gctcaccacc
     1321 ggcctcggct tcctgctggc ccaggagagc ggcgtcgccg tcgtcctcct gctgcacacc
     1381 ctgctgggca cgggcctgat ctccggcggc gcctcggtgc tcaatcaggt gctggagcgc
     1441 gacgtcgacg ccctcatgcg gcggaccgcg aaccggccgc tgccggccgg ccgcctcgat
     1501 ccggacaccg ccctcctctt cggggtgtcg ctcgccgtcc tcggcctggt ggagctggtc
     1561 ttcttcgtca acctcctcac ggcgctcctg ggagccgcgg ccctcgccgg ctacgtcttc
     1621 ctctatacgc cgctcaagcg ggtgacgtcg ctcgccacgg tggtcggcgc ggtgcccggc
     1681 gccattccgc cgatgatggg gtggagcgcg gtgcgcggag agctggacct gccggcgtgg
     1741 gtgctgttcg gcatcctgtt cttctggcag atgccccact tcctggcgat cgcctggctc
     1801 tgccgcgagg actacggcaa cgccggcttc cccatgctca cggtcgacga cccggacggc
     1861 cggcggaccg cgcggcaggc catcctctac tgcgcgccgc tggtcccgct gagcctcctg
     1921 ccgtcggtgc tcggcctcat ggggacggtc tatttcatcg gcgccctggc cctggggctc
     1981 gcctatctgg gcttcgcctt cggctttgcc cgccaccgct ccaccgccgg agcgcgcggg
     2041 ctcatgttct cgtccctcct ctatttcccg ggagtgctgc tggtgatgct cctggaccgg
     2101 atcgtctcct gatccggacc ccgcgaggtt cggccggatg gtcgtgacgg agctttcgag
     2161 tcccgggacc gggccgacgg agccgatgga gccgatagcg cggcgggcgc aggcggtccc
     2221 cgccgtcggc gcgcagggcg tcgagttctc ctaccccgg
//
LOCUS       DASPJI010000639        24100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_98070, whole genome shotgun sequence.
ACCESSION   DASPJI010000639 DASPJI010000000
VERSION     DASPJI010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 24100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24100)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24100
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98070"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..888
                     /locus_tag="VEL74_17985"
     CDS             232..888
                     /locus_tag="VEL74_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HYG64474.1"
                     /translation="MTKLKTQCQYSAGEEIAHCTSHGFGLLLSIAGLVLLIDLALVRG
                     GGRLVTACSVFGVTLILLYLASTLYHGIRHERAKRVLRVLDHSAIYLLIAGTYTPFCL
                     VSLGGAWGWTLFGIVWGLAFLGISLKVMDIGRLRWLSIVLYVGMGWLVLVALDPMIAA
                     VGKGGMWLLLLGGIAYTSGIVFYAWRRPYHHAIWHGFVLAGSILHFLAVLLYVIPPAG
                     "
     gene            1031..2140
                     /locus_tag="VEL74_17990"
     CDS             1031..2140
                     /locus_tag="VEL74_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HYG64475.1"
                     /translation="MLQQNVQRGVRIVGAGAFVPSLAVDNHRIAEAIPGWPAERIEEK
                     TSIRERRFLWDFDERTGRAVPPPEGSDQPRSNADMCEIALRRALDKAGLDAKELDAVF
                     LVTCTPDRLNFNHDAMEVHRRLGCRSDAYALVIDDGCGGTPYVMDMAYKMIKSGAVRT
                     VAVIGSAFSSPQLNREVFTSWVEPAPGRKPINAYFSMYVFGDGAGAVVLRGGGLPGEG
                     ILSSMSGNDYMDLVHRRAGGFLQLPYQGETHPGDHAFVVDGQLVARTYPLYMQRCLDE
                     VLSECPELKGQVARYYFHQPNKRLMDRFIQSAALPAERVAYNVDRYGNTSAAGMLILL
                     AEDLDAGVVRLGGGDLVLIAAVGANVHYGAQLVRL"
     gene            2153..2593
                     /locus_tag="VEL74_17995"
     CDS             2153..2593
                     /locus_tag="VEL74_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64476.1"
                     /translation="MKVYRFRSQEDPAAPPDPVVCAAAPFEPNIRLGATLWEGPAGSE
                     QRIGIATACARATDPSFEPGARLPFHAVFHLPEGTVVATGECTVVSNDVPVPGLVLAA
                     CHLRVVGAPAGFAGGAATSLSVFNPRGLEGFATGSFWDVVLFPS"
     gene            2627..3229
                     /locus_tag="VEL74_18000"
     CDS             2627..3229
                     /locus_tag="VEL74_18000"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HYG64477.1"
                     /translation="MDITHVFFDIGGVLGSNGWDREQRARAVEAFGLGEEFERRHGEL
                     VGELEAGRMTLDEYLESAVFYCPQPCTRPEFREFMLAQSAPFPDSIEVARELTGNPRL
                     RLMTLNNESEELNVHRIERFGLRPIFSAFLSSCWLGARKPSRLIFQRALGISQAEPGR
                     ALFIDDRDQNLAPARLLGFQTLLFTDAVSLRQALASRGLL"
     gene            3260..4681
                     /locus_tag="VEL74_18005"
     CDS             3260..4681
                     /locus_tag="VEL74_18005"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64478.1"
                     /translation="MSIKPVVFVPGFPATELIQASKNRRIFPPDPGDLLDGGKKEKLI
                     RLLSGPDNPPGDIVAGDPIRDVLGISKQAESLYDLLRDYGYTAHSGDNFAAVGWDWRL
                     AVDDIRVQTALIAAVDRLSRAHNSKVVVICHSTGGLVVRRLLEMRPDVADQIEQIFAF
                     GVPWAGTLKAVRYLGRGESFGFLFAKLSASEVREIMSHCQAAYDLFPPDPAKTDMRDL
                     TGQDLNLFVDAELPRGQQQVGPLVDLRWVPKSSGKDYMRQMANLADSRLGRRPNQIRL
                     PNTPTPPITNVVGWGAATDTTCEMDAKGRLTFLETKEGDGTVPAVSASWLRGPDVRTF
                     FLPIGLYPTAGIPNRHSRIWDSPPVLELFDQVLRSKAPEPWVCACVDGDQGIDPSGPV
                     TLRLVAADDQGRPLPNARGNFVGLPQRVAFSFSGAARTSLILRRGQFRPNVGRNLFRF
                     IVEIIWDTGSGPRTREIPILIHV"
     gene            4701..5516
                     /locus_tag="VEL74_18010"
     CDS             4701..5516
                     /locus_tag="VEL74_18010"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYG64479.1"
                     /translation="MRPKIWKGFALVLGIGVPLFMLDAFARSALYPAPSVPVPSPPPP
                     SLEEVRLDLSTGETVVGWARAAPSLPPDAPVVLFFHGNGENLQTMNWAGMFEDLEQLG
                     VAYLAVDYPGYGRSSGKPSEEGLMATGDAAVAWARRQHSGRPVVLCGWSLGAATVIPT
                     AARHPEDVRGLIALSPWTSLKDVASHHFPAFLVKALLRERYDSLEAARRIRVPALVIH
                     GDLDDLIPAAQGKLVAEALAGPKKWVSLPGAGHNDLFARPEVWREIEAFLRSI"
     gene            complement(5522..6313)
                     /locus_tag="VEL74_18015"
     CDS             complement(5522..6313)
                     /locus_tag="VEL74_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HYG64480.1"
                     /translation="MEPTDILALAGAAFAAGVMNSMAGGGTILTFPTLIFLGLPAITA
                     NATSTVALLPGVVAAMAGYRKEVAAHRDWLKTLFLPSLVGGAAGSVLLLMTPEKVFAN
                     LAPLLVLFATVLFMVQGVVARRRAEAGNEGDLPDPGTLPRRRLIIASLLQLAVAVYGG
                     YFGAGIGILMLALLGFLGLSDIHAMNGLKVFFNVCINSVAAVYFVARGAVDWPVALVL
                     AIGAIAGGYGGARLARRIGQKKARAAVIVIGLFVTAVLFWQQLRG"
     gene            6502..8583
                     /locus_tag="VEL74_18020"
     CDS             6502..8583
                     /locus_tag="VEL74_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64481.1"
                     /translation="MTRKLVWLMLLAVGGAVHAAPPPLVPTPTANLHEVLDVNQVDVV
                     LPAQLDLEKIGAEDLERDEIGLPPRFAVPERVSITPGKNGTWEDLADGQRLWRLRVVG
                     GVGTTSLNLGFTRYNMPPGGRLLLYSADGVQIGRPFTAEDNEAHGELWSPVVLTNDLI
                     VEVTVPKRQARALELELGWINQGYRGFGTVPAAAYEKSGSCNLDVECLDVADTWREEM
                     RSVGVISTGGSTFCTGSLVNNTANDRRMYFITANHCGISSSNAASLVVYWNYQNSFCR
                     APGSAASGQAGDGLLNQFHTGSFFRASYSPSDVTLVELDDPAVNLYNHHWAGWDRSTG
                     SHTCTAASPCASIHHPSTDEKRITYVITDMVSTSYNSTASPGDGSHLWAKWATDPPGP
                     YTVPGVTEPGSSGSPLYNSAGRFVGQLHGGPSSCGATGNSLSDYYGRFSVSWTGGGTN
                     SSRLSNWLDPGNTGATTIGGIDVFTGPCTPPAAPTGVSASAVSANQINLSWSAAAGAT
                     SYPVLRSTTNGGPYATAGIAPTTSFSNTGLTCNTTYYYVVTSSNGLCSSGNSVQVQAT
                     TAACPICTNTTETEQNNSRTSPQVISGSCNQISGSFLNDKKSGQEDYFRLSLPAGATV
                     TALLNGLSADYDLYIYNSTSGAEVASSLNGGTTADQASWTNAGASAVNVFVRVYRYSS
                     TRTTYQLRVSY"
     gene            8654..9958
                     /locus_tag="VEL74_18025"
     CDS             8654..9958
                     /locus_tag="VEL74_18025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYG64482.1"
                     /translation="MKIVLTMNLPWFPAVGGANKCNRVLAEGLAGLGHSVRAVVPALG
                     VPSRITLEEWRRMLADQGFASRQEDGVDVFSWNGVEVHAVAEPSRLRAELIERLRGFQ
                     PDWALVSSEDPSQNLLDAALQAQAAPIVYLAHTPAFLPFGPQAFYPSERRGRRLGQVA
                     GIVTVSRFMAEYIRRWGGLHADVFHFSVYGPGPFPDFGSEGSGEGFVTLINPCAVKGI
                     AIFLGLARELPEVRFAAVPTWGTTTADRAALAAVPNVTLLKPEVDIDRIFARTRVLLM
                     PSLWQEAFGVTAVEAMLRGLPVLASDVGGLPEATLGAGRVLPVRPIERFLDRQDDNEV
                     TKAVVPEQDLTPWRDALRELLGDRGLYPRESARAREAAHRFNAGLGAGPFVEILDRTP
                     RHAPEAAAAAPAPEAEAGGVAELTPEQRALLMLRLRKKTAQK"
     gene            9992..11788
                     /locus_tag="VEL74_18030"
     CDS             9992..11788
                     /locus_tag="VEL74_18030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG64483.1"
                     /translation="MLSQRQSVLLAAFLFLPLNPASAQPEPGAFELLAKARQAYAQLP
                     SYRDHGEIETTLSAGVTVRHRFELTAAPGAFRFLLERDLGGEPQRRMIWRSGGEAFHF
                     DSCLGQYKTVASLTDEVAGALGSGGADALVVPITLVGGAGALADPEVATVEGEEPCGD
                     GSCWIVFFSRATDVESRLWIDSRSFLIRQVEVRLQDTGDVVDQAMAQAGLAVRGTAPG
                     AARGTITIRVRHEIEAGKPVQRAEAAFGPPPGARRVTEWQVAGDDELPQFGEEITVAI
                     AAVTVRVVDNHGRPVEGLAPGDFRVLAKGKEIPVVAVDWVSSARTEAPLDPDGSAVSS
                     GPSAPAPSGEQPCKRVVFFVQSGNHPTRLKGQMRLRPHTRELLESFPAADRLAVVSYD
                     SHLKLWQDFTDDREAVFQAIDQAMLFGGDRQTVVRPSRDRSSLAAHLDFDAALRTASP
                     ERALQVTAEALQPLPGEKVMIYVGWGLGRFGSDGVRMTPDYRPAIEALDAARVTVFVL
                     DITDADYHSLETGLQSVAYQTGGSYAKTHIFPQHATDFLAGTVSGHYVLTLDRTALPP
                     QGSRVRIHLRDKKGTVYYARPDPVPGVGDTGR"
     gene            11858..12370
                     /locus_tag="VEL74_18035"
     CDS             11858..12370
                     /locus_tag="VEL74_18035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG64484.1"
                     /translation="MSADARDFESLLQRYARLMASAVRKVCGREHRDLVPDVEQEIYL
                     ALWKRLQDGKEIRHPVSYLYKVALTTALAFVRRRGGEATTNDGGELERPPTPLPGADG
                     LLPAERRLLLEQVLRELPREQARAVRAYLAGFNHQEVAALYGWSEPVARHRIYRGLES
                     LRGKLVETSR"
     gene            12367..13062
                     /locus_tag="VEL74_18040"
     CDS             12367..13062
                     /locus_tag="VEL74_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64485.1"
                     /translation="MSDEPMDEAEGAAWREAYRGFQAPGSSGCPDDEALAALATGETT
                     GAERERLADHVVGCRRCAERYRVLRDLHREAGSEPEHEGAGSGGRGSRLRLLAAAAAL
                     LLVAGLGWIVGLPGTSGVLDPDRLRGEPAMEEGVRPAPDARLAAPPRELAWPARRGAH
                     SYRVILYDATSSPLWTSAPVTAPILTLPDEARSRLVGGDSYFWIVETGHGPDAPVSRL
                     GPFWFSLEERRER"
     gene            13059..16271
                     /locus_tag="VEL74_18045"
     CDS             13059..16271
                     /locus_tag="VEL74_18045"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYG64486.1"
                     /translation="MSWRAFLSILVLLAAAVAASFALGASRESLRETTGAGLLVEEVG
                     DGSSMAEAGIRAGDILLSWERPAAHGEPPARGPLRSFFDLQLMGLEQSSRAKVRVTGA
                     RLGRTLTWTLAEGEWRITVRPLMPPLLESGYAAGRRRLAAGEAGRAFAQWNQTAEAAA
                     AAGQPVLAAWLWRQVASEALERQLWDEAERVLARGLALPLPAAVRARLLTFQAQAFDR
                     QGELLQAEAALRAALAAQAGTPPGLALAADLDDLGWALIRRGDLDGAGAAFRRALALR
                     LRLAPGSLLLAASYNKLGILHMERGDLAAAEEMFRRVVGLWESRGPHSLAMAAGLENL
                     GLVTTYRGDLAAAAGLMRRAAALKQRLDPDSEHVGRSLLNQGMLQWRRGDLSSAQDLL
                     TSALGLWRQLSPRSRDLAGVLTNLGLIALDRGELGLAERRFREALALWRELEPGTPTV
                     AGTLNNLAMVARRQGDPVGAERLLREALALEEKAAPQGLAAALYLQNLGELAEDQGAL
                     EEAEGLMRRALSISSRLAPGSELEAKSWYGLGRLHRRAGRPAEAREAWMRGVAALEEQ
                     EAKLGGTEEIRSAWRGKHGGLYHDLLELLLEQGRADEAFQLSERYRARRLLALLAERD
                     LLWREDLPAGLARERSRLAVEHDRIQAALAQLDAGAADARAQVELLGRQLRELRNRRA
                     ELADAILAASPRLASLQYPRPLDAAAAQAALAPGTILLAYAVGLRHSHLFVLERDGPL
                     RVFLLPVGEDGLRDRILGFRHLLRQPAGGTRSRAALEERGQTLARDLLAPALPHIARA
                     DRILILADGPLHALPFAPLPVAGRPAASRFLVEWKPLHTAASATVYAELRKRRRPSGS
                     RGASRAGLVIFGDPRNARDLDGRPLPPLPATRAESRALERLFPGARVFLGGDATEEAL
                     KAAGRSARYLHVASHAVTDEHLPLDSYLALSSPRRPRPGQDNGLLQAWEIFEQVRLDS
                     DLVTLASCSTALGRELAGEGVLSLARAFQYAGARSVVASLWDVPDRSTSLLMQRFYAG
                     LARGLAKDEALRRAQVALLKRPETSHPFHWAAHQLAGDWH"
     gene            16389..16850
                     /locus_tag="VEL74_18050"
     CDS             16389..16850
                     /locus_tag="VEL74_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64487.1"
                     /translation="MRIAVLIRKLSTAGALTLLAATAAHAAPLDFKDVMAVASTRDFL
                     KIDDLQVVVSRPPFDACVDWNLALCIEEVWHESWVVCHQPWGQEKLVALSCGALRTVT
                     KRCADRTWHEDVDYATGGRYCVESPEHQYLCDNGPGIVQSQDVRISSRPCA"
     gene            17470..18747
                     /locus_tag="VEL74_18055"
     CDS             17470..18747
                     /locus_tag="VEL74_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64488.1"
                     /translation="MKRRVHASIVLLLVVSLLGMPMASMAQKRGRDAKQLGRHLRQGE
                     GVTQAIQQVVGPMPGIAIAPFLGLAALSGLALLADTEYARTSDNTFVRAVCDNSLVRE
                     AQKYSSLTLFLLLCGLALLTYLASSGKIRGIVGKMLRATEDGVVTPTYALLSLGAFSG
                     LSMVAQPAVAEMGFSLPSAVLLAFGVAFGLAAMMTVRFAFDVLIWLSPVPFIDFLFET
                     AKNVFSVGLLVLYLFFPGFATAVAVLFLAVSLLLYGWAIRIVGFAFQIVLRPVLARLV
                     PSLRTRLLDEKRAGHAGVAGARIATPAAALAVPGFSKRQLGTLFLTTDGLSFSARPLL
                     GRTRQQPLLLRPDTRPVLCRCLFWYEVRVTSPEGRLYKLALPKTLQPEWTALQTLLAA
                     EDAGAIGGADKLRKLADRMGEQLKGMPPTAQAR"
     gene            18870..19049
                     /locus_tag="VEL74_18060"
     CDS             18870..19049
                     /locus_tag="VEL74_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64489.1"
                     /translation="MDQLQWADWDRDGNLLVATRSGKLEIWNLARDRTEVLFEEDLSL
                     LAPNPVPAPDWARQG"
     gene            19172..19708
                     /locus_tag="VEL74_18065"
     CDS             19172..19708
                     /locus_tag="VEL74_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64490.1"
                     /translation="MNRSLLPLAVLLGAALFSGPTYAATVNVSALWCGFGCSEGHNDT
                     QCICTYDSNYSPSSPWAWISCEEVRCCDTEYAYCDLGYVFEGYDGTCSWRPGTETCAT
                     PTPNGGWSNCEVQCGSSGCWAPYRVAHWETVNEGSPDYCTRSNETGDWWNPWTWTTPC
                     TLEDQSPWDDPGSGLVCG"
     gene            19793..21133
                     /locus_tag="VEL74_18070"
     CDS             19793..21133
                     /locus_tag="VEL74_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HYG64491.1"
                     /translation="MPVRTQSPPGERRPLSSILALRRDPLGYLEGLAARYGDIATIQV
                     GSYRLFFLNHPDLIHEALATRDASLVKGRILDQARDVFGQGLLTSEGDFHRKQRRLIQ
                     PAFHRRRIEAYADAMAADAAAAAARWRHGETVDVATEMMRLTLRIVARCLFSVDVEDD
                     EAREVGGALDALMAAFSTTVLGSGFIRKLPLPAFRRARRGVKTLDALVYRMIAERRRT
                     GGGGGEDLLSLLMAAREEGEGSGMDDRQVRDEVLTLFLAGHETTANALAWSWFLLANH
                     PEAEASFHAEIDSVLGDRLPGAADLPSLPYNRKVFTESMRLYPPAWAISRRAAEPLTL
                     GGYPVAAGSGVLASQWVMHRDPRYFEDPLAFRPERWTEEFESRLPKLAYFPFGGGPRT
                     CIGMGFARMEGVLLLATLGRRWRMRLVPGQRIEPSPRITLRPKNGIRVTLERRG"
     gene            complement(21111..21497)
                     /locus_tag="VEL74_18075"
     CDS             complement(21111..21497)
                     /locus_tag="VEL74_18075"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYG64492.1"
                     /translation="MPVRSLHSRVLRWPRPAEVEEALRAWAGREAPRHAGLLRLGWFG
                     SYARGDAGVGSDLDLIAVVEESGKPFERRALDWDLLPLPVPAEILIYTRAEWGKLRQQ
                     GGRFIRTIEREARWLPLPQVSPAAPG"
     gene            complement(21454..21834)
                     /locus_tag="VEL74_18080"
     CDS             complement(21454..21834)
                     /locus_tag="VEL74_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019551403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HYG64493.1"
                     /translation="MNRSRDWLAQAERDLAQAEASRQQGHHEWACFAAQQAAEKAVKA
                     LNLHLFQEAWGHVVARLLEELPIEVDPLLVQKAKVLDNFYIGTRYPDGHAEGPPFEHY
                     GEIQSRDAINYAGEILAFARTQMA"
     gene            21944..23326
                     /locus_tag="VEL74_18085"
     CDS             21944..23326
                     /locus_tag="VEL74_18085"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYG64494.1"
                     /translation="MRRHSRVTPIRNGLALLVVLVSLLTLPGCREAEPPAPPQPSKPR
                     LFFLGVDGVSWTVLGPMLERGELPAFQRLIEEGTSLPRFDTLQITHSPVIWTTVATGR
                     RPAVHGIRDFTAELPNGRKIPVTSNMRKARAIWEVASRRGVSAGVIGWWATWPAEEIL
                     GYVVSDHANPAFGDVMTAEGRFWTADRERLAQLRRQVYPEEIAPVLARYWITGETFPW
                     DDLQRRGRFTDEQMRLARAAAWAEPDLYSWLKTFYRVDYPLFRAALDLMKERPTDLQM
                     LYLRGPDPVQHYGWDLVEPERYARKPPHLERDRTVVEGVYRYVDSFLGEILDALPPDA
                     WLIVASDHGAEPSDDAADPARTSRPGEHSVEAKGVLFLRGPGVRRGANLEKATPFDLM
                     PTMAWLLELPLSSTLPGRILTEAFEEDFARSLPVHKVARYGPRPTGPLLPSPEDEEML
                     RSLKNLGYIQ"
     gene            23572..>24100
                     /locus_tag="VEL74_18090"
     CDS             23572..>24100
                     /locus_tag="VEL74_18090"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYG64495.1"
                     /translation="MFQRSRPMLLALLLMVGAVAGVFAQTSSTTGEISGRVVDETGGV
                     MPGVTVTATNTQTGLSRTAVTEEDGDYSLGLLPPGIYRVAAELSGFRSDVRENVTVLL
                     GVSSNVRFTLSAELSEEITVTSQAPLVDVTDADLTLSVTQDQIENLPILGRDFSDLVL
                     LTPGAGEAFGDRVALN"
BASE COUNT         3382 a         8694 c         8240 g         3784 t
ORIGIN      
        1 cgacatcccc atgcagccca ggccgagggc agacacctcc agcccctgcg tccccaactt
       61 tctcgtctcc atccgtccac ctcctcaccg gccgggaccg cccgccgcgg gaccttcctg
      121 cgcggcgccc ggcacgagag ggacagtcta tccctgcggc gccggtccga tccggccgaa
      181 atcggcgaca atgggtctcc ctgctggagc cggtggggga ggaggggtgc catgacaaag
      241 ctgaagacgc agtgccagta ctcggcgggt gaggagatcg cccactgcac ctcccatggt
      301 ttcgggctcc tgttgagcat cgccgggctg gtcctgctga tcgacctggc gctggtgcgc
      361 ggcggcgggc ggctggtcac cgcgtgcagc gtgttcggcg tgacgctgat cctcctctat
      421 ctcgcctcga ccctgtatca cggcatccgc cacgagcggg ccaagcgcgt cctgcgggtg
      481 ctcgaccact cggccatcta cctgctgatc gccggcacct acacgccgtt ctgtctggtg
      541 agcctcggcg gcgcctgggg ttggaccctg ttcggcatcg tctgggggct cgcctttctg
      601 ggcatctcgc tgaaagtgat ggatatcgga cgcctgcgct ggctctcgat cgtcctctac
      661 gtcggcatgg gctggctggt gctcgtcgcg ctcgatccga tgatcgcggc ggtggggaag
      721 gggggcatgt ggctcctcct cctgggcggc atcgcctata cgtcgggcat cgtcttctat
      781 gcgtggcggc gaccgtatca ccacgccatc tggcacggct tcgtgctggc cgggagcatc
      841 ctgcacttcc tggccgtgct gctctacgtc atccctccgg cgggatgaaa ccacctctcc
      901 tcgaagtctc cccagcctga ggggatcgcc cggcggcggg cgttttcctc ggcgtggccg
      961 gtgcaggacc ggctcccgac acagacgaat ccgccggggt gaccggcttc gagacttcga
     1021 gaggtagttc atgctccagc agaatgtcca gcgtggcgtc cgtatcgtgg gcgccggtgc
     1081 tttcgtcccc tccctggccg tcgacaacca ccgcatcgcc gaggcgatcc ccggctggcc
     1141 ggccgagcgc atcgaggaga agaccagcat ccgcgagcgc cgcttccttt gggacttcga
     1201 cgagcggacg ggccgcgccg tgccgccgcc cgagggctcg gaccagccgc ggtccaacgc
     1261 cgacatgtgc gagatcgcgc tgcgccgcgc cctggacaag gcgggcctcg acgccaagga
     1321 gctggacgcc gtcttcctgg tgacctgcac cccggaccgc ctcaacttca accacgacgc
     1381 catggaggtg caccgccggc tgggctgccg gagcgacgcc tacgcgttgg tgatcgacga
     1441 cggctgcggc ggcacgccgt acgtcatgga catggcctac aagatgatca agagcggcgc
     1501 ggtccggacc gtggccgtga tcggctcggc gttcagctcg ccgcagctca accgcgaggt
     1561 cttcacctcc tgggtggagc cggcgccggg acgcaagccg atcaacgcct atttctccat
     1621 gtacgtcttc ggcgacggcg cgggcgcggt ggtgctgcgc ggcggcggcc tgcccgggga
     1681 ggggatcctc tcctcgatgt cgggcaacga ctacatggac ctggtgcacc gccgggccgg
     1741 cggcttcctc cagctcccgt atcaggggga gacccacccg ggcgaccacg ccttcgtggt
     1801 ggacggccag ctcgtcgcca ggacctatcc gctctacatg cagcgctgcc tcgacgaggt
     1861 gctctccgag tgccccgagc tcaaggggca ggtggcgcgc tactacttcc atcagcccaa
     1921 caagcggctg atggaccgct tcatccagtc cgccgccctg cccgccgagc gggtggccta
     1981 caacgtcgac cgctacggca acacctcggc ggccgggatg ctgatcctcc tcgccgagga
     2041 cctcgacgcg ggcgtggtgc ggctgggcgg cggcgacctg gtgctcatcg ccgcggtcgg
     2101 cgccaacgtc cactacggcg cccagctcgt ccgcctgtaa ggatagggag ggatgaaggt
     2161 ctatcgcttc cgttcccagg aggatccggc ggcgccgccc gacccggtgg tctgcgcggc
     2221 ggcgcctttc gagccgaaca tccggctggg cgccacgctc tgggagggcc cggccggctc
     2281 cgagcagcgg atcggcatcg ccacggcgtg cgcccgagcc acggacccgt cgttcgagcc
     2341 cggggcgcgg ctgccgttcc acgccgtgtt ccatctcccc gagggcaccg tcgtcgccac
     2401 cggcgaatgc acggtggtga gcaacgacgt gccggtcccc ggcctggtgc tggccgcctg
     2461 ccacctgcgg gtggtgggcg cgcccgccgg cttcgcgggc ggcgccgcca ccagcctgag
     2521 cgtcttcaac ccccgcggcc tggagggctt cgccaccggc tcgttctggg acgtggtgct
     2581 gttcccgagc tgacatatcc tgttcctctc gacgggaggg cagcgcgtgg acatcaccca
     2641 tgtgttcttc gacatcggcg gcgttctggg gagcaacggc tgggaccgcg agcagcgggc
     2701 gcgggcggtg gaggcgttcg ggctcggcga ggagttcgag cgccggcacg gcgagctggt
     2761 gggagagctg gaggcggggc ggatgaccct cgacgagtac ctcgagagcg ccgtcttcta
     2821 ttgtccgcag ccgtgcaccc gcccggagtt ccgggagttc atgctggccc agagcgcgcc
     2881 gttccccgac agcatcgagg tcgcccgcga gctgaccggg aacccgcgcc tgcgcctgat
     2941 gacgctcaac aacgagtccg aggagctgaa cgtccaccgg atcgagcgct tcggcctgcg
     3001 cccgatcttc tcggcgttcc tctcctcctg ctggctcggc gcgcgcaagc cgtcgcggct
     3061 gatcttccag cgcgccctgg ggatctcgca ggccgagccg ggacgggcgc tgttcatcga
     3121 cgaccgggac cagaacctgg ccccggcgcg cctcctcggt ttccagacac tcctctttac
     3181 tgacgccgtc tccctgcggc aggccctcgc ctcccggggg ctgctctaga acgaaagcct
     3241 ctttccggag atcaaggcca tgtccatcaa gccggtcgtg ttcgtcccgg gcttcccggc
     3301 caccgagctg atccaggcgt cgaagaaccg caggatcttc cctcccgatc ccggcgacct
     3361 cctggacggt ggcaagaagg agaagctgat ccggctgctg agcgggcccg acaatccgcc
     3421 cggcgacatc gtggccggcg atcccatccg ggacgtgctg gggatctcca agcaggccga
     3481 gtcgctgtac gacctcctgc gcgactacgg ctacaccgcc cactcggggg acaacttcgc
     3541 ggcggtcggt tgggactggc gcctggcggt ggacgacatc cgggtgcaga cggcactgat
     3601 cgccgccgtc gaccggttgt cgcgggccca caattcgaag gtggtggtga tctgtcactc
     3661 cacgggcggg ctggtcgtcc ggcgcctgct cgagatgcgt cctgacgtgg ccgatcagat
     3721 cgagcagatc ttcgccttcg gcgtcccttg ggcggggacg ctcaaggcgg tccgctacct
     3781 gggccggggg gaatcgttcg gatttctgtt cgccaaactc tcggcgagcg aggtgcggga
     3841 gatcatgagt cattgccagg cggcctatga tctcttcccg ccggacccgg ccaagacgga
     3901 catgcgcgac ttgaccgggc aggatctgaa tctgttcgtg gacgccgagc tgcccagggg
     3961 ccagcagcag gtcggacccc tggtggacct gcggtgggtg ccgaagtcat ccggcaagga
     4021 ctacatgcgg cagatggcca atctggccga tagccggctc ggccgccgtc cgaaccagat
     4081 ccgcctgccc aacacaccca cgccgccgat caccaacgtc gtgggctggg gtgcggccac
     4141 ggacaccacc tgcgagatgg acgcgaaggg acggctgacg ttcctcgaga ccaaggaggg
     4201 ggacggcacc gtgcccgcgg tgtcggcgtc gtggctgcgt gggccggacg tgcgcacctt
     4261 cttcctgccg atcggtctct atccaaccgc cggcatcccc aatcggcact cccggatctg
     4321 ggactcgccg ccggtgctcg agctgttcga ccaggtgctg cgttccaagg ctccggagcc
     4381 ctgggtctgt gcctgcgtcg acggcgacca ggggatcgac ccgagcggac cggtgaccct
     4441 ccggctggtg gcggcggacg accaggggag gccactgccc aacgcccgcg gcaacttcgt
     4501 cggcctgccg cagcgcgtgg cgttcagctt cagcggcgcc gcccgcacgt cgctgatcct
     4561 ccggcggggg caattccggc ccaacgtggg ccggaatctc ttccgtttca tcgtcgagat
     4621 catctgggac accggcagcg gcccgcggac ccgcgagatc ccgatcctga tccacgtgta
     4681 gcaaggtggt aggcttgctc atgcggccga agatctggaa gggttttgcc ctggtcctcg
     4741 gaattggagt ccctctcttc atgctcgacg ccttcgcccg ttcggctctc tatcccgccc
     4801 cgtccgtacc ggtcccgtcg ccgcctccgc cgtcactgga ggaggtgcgg ctcgatctct
     4861 cgaccggaga gacggtggtg ggctgggccc gtgccgcgcc ctctctgccg ccggacgccc
     4921 cggtcgtcct tttctttcat ggcaacggcg agaacctgca gaccatgaac tgggcgggga
     4981 tgttcgagga cctggagcag ctcggcgtcg cgtatctggc ggtcgattat ccgggctatg
     5041 gccgcagctc gggaaaaccg tcggaggagg ggctgatggc cacgggcgac gcggccgtgg
     5101 cctgggcccg gcgccagcat tccggccggc cggtggtgct ctgcggctgg tcgctgggcg
     5161 ccgcgacggt gatccccacc gccgcccgcc accccgagga cgtccggggc ctcatcgccc
     5221 tgtcgccgtg gacgtcgctc aaggacgtgg cttctcacca tttccccgct ttcctggtga
     5281 aggccctgct gcgggagcgc tacgactcgc tggaggcggc gcgccggatc cgcgtccccg
     5341 cgctggtgat ccacggcgac ctcgacgacc tcatccccgc cgcgcagggc aagctggtcg
     5401 ccgaagccct ggccggaccc aagaagtggg tttcgctccc cggcgccggc cataacgatc
     5461 tcttcgcccg cccggaggtg tggcgggaga tcgaggcctt cctgcgttcc atctgagagg
     5521 ttcagccgcg gagctgctgc cagaacagca ccgcggtcac gaacaggccg atgacgatca
     5581 cggcggcgcg tgccttcttc tggccgatgc gtcgggcgag gcgggcgccg ccgtagccgc
     5641 cggcgatggc gccgatggcg agcaccaggg ccaccggcca gtccaccgcc ccccgcgcga
     5701 cgaagtagac tgcggccacg ctgttgatac acacattgaa aaacaccttg agcccgttca
     5761 tggcgtggat gtccgacaac cccaggaatc ccaggagcgc cagcatcaga atgccgatcc
     5821 cggcgccgaa gtagccgccg tagaccgcga cggcgagttg gaggagggac gcgatgatca
     5881 gccgccgccg ggggagggtg cccgggtccg gcaaatcgcc ctcgttgcct gcctcggctc
     5941 gccgtcgcgc gaccaccccc tggaccatga acagcaccgt ggcgaacagc accaggaggg
     6001 gagcgaggtt ggcgaacacc ttctccggcg tcatgagcag cagcaccgat ccggcagccc
     6061 cgcccacgag gctgggcaag aagagggtct tgagccagtc ccgatgcgcc gccacctcct
     6121 tccggtagcc ggccatggcg gccaccacgc cggggagcag cgccacggtc gaggtggcat
     6181 tggcggtgat ggctggcagc cccaggaaga tcagggtggg gaaggtgagg atcgtgccgc
     6241 cgccggccat ggagttcatc accccggcgg cgaaggcagc gcccgcgagc gccaggatgt
     6301 cggtcggttc catgggatcg gtctccaggc gccctcggag gacggcgcgt ggggattcta
     6361 gtgcctcttg gtgccggatc accgggggcg gtgtcccgct gttccggtct cctgcaccct
     6421 catgagtaag ggctcccggg tagattcggc cgcggtcggc gcccgcatgt catcccgtgc
     6481 ctttccacaa gaggagaaga gatgacccgt aagctggttt ggctgatgtt gttggccgtc
     6541 ggcggcgccg tccatgccgc ccccccgcct ctcgttccca ctcccaccgc caaccttcac
     6601 gaagtcctcg atgtcaacca ggtcgatgtc gtcctgccgg cgcagttgga tctggagaag
     6661 atcggggcag aggacctgga gcgggatgag atcgggctgc cgccgcgctt cgcggttccg
     6721 gagcgggtct ccatcactcc cggcaagaac ggtacctggg aagatctcgc cgacggccag
     6781 cggctctggc ggctgcgcgt cgtgggcggc gtggggacga cctccctcaa cctcggcttc
     6841 acccggtaca acatgccgcc ggggggccgc ctcctcctgt actcggcgga cggcgtccag
     6901 atcggccgcc ccttcacggc cgaggacaac gaggcgcacg gcgagctgtg gtcgccggtg
     6961 gtcctcacca acgacctgat cgtcgaggtc accgtgccga agcggcaggc gcgggcgctc
     7021 gagctggagc tcggctggat caaccagggc taccgcggct tcggcacggt cccggccgcc
     7081 gcctatgaga agtccgggag ctgcaatctc gacgtggagt gcctggacgt cgccgacacc
     7141 tggcgcgagg agatgcggtc ggtcggtgtg atctcgaccg gcggctccac cttctgcacc
     7201 ggctcgctgg tcaacaacac ggccaacgac cgcaggatgt atttcatcac cgccaaccac
     7261 tgcggcatca gctcgtcgaa cgcggcgtcg ctggtggtct actggaacta tcagaactcc
     7321 ttctgccggg cgccgggcag cgccgccagc ggtcaggccg gcgacggcct gctcaaccag
     7381 ttccataccg gctccttctt ccgcgcctcc tactctccct cggacgtcac gctggtggag
     7441 ctcgacgatc cggcggtcaa tctctacaac caccactggg ccggctggga ccgctcgacc
     7501 ggcagccaca cctgcaccgc cgcctccccc tgcgcctcga tccaccaccc gtcgaccgac
     7561 gagaagcgga tcacgtatgt catcaccgat atggtcagca cgagctacaa cagcaccgcc
     7621 tcgccgggcg acggcagcca tctgtgggcg aagtgggcga ccgatcctcc gggtccctac
     7681 accgtgccgg gcgtgaccga gcccggctcg tccggctcgc cgctgtacaa cagcgccggc
     7741 cgcttcgtcg gccagctcca cggcggcccc tcctcctgcg gcgccacggg caacagcctg
     7801 tccgactact acgggaggtt cagcgtctcc tggaccggcg gcggcacgaa cagcagccga
     7861 ctctccaact ggctcgatcc cggcaacacc ggcgccacga ccatcggcgg catcgacgtc
     7921 ttcaccggcc cctgcacgcc gcccgcggct cccaccggag tgagcgcctc ggccgtgtcg
     7981 gcgaatcaga tcaacctttc ctggagcgcc gcggcgggtg ccacctcgta tccggtgctg
     8041 cgctcgacca ccaatggcgg gccgtacgcg acggcgggca tcgcgcccac gacgtccttc
     8101 tcgaacacgg ggctcacctg caacaccacc tattactatg tggtgacgtc gtcgaacggt
     8161 ctttgcagct ccggcaactc ggtccaggtg caggcgacga cggcggcctg cccgatctgc
     8221 accaacacca cggagacgga gcagaacaac agccgcacct cgccgcaggt gatctccggg
     8281 agctgcaacc agatctcggg gagcttcctc aacgacaaga agtcggggca ggaggattac
     8341 ttccggttga gcctgccggc gggcgccacg gtgacggcgc tgctcaacgg cctgagcgcc
     8401 gattacgacc tctacatcta caactcgacg agcggcgccg aggtggcgtc ctcgctgaac
     8461 ggcggcacga ccgccgacca ggcgtcctgg accaacgccg gcgcttcggc ggtcaacgtc
     8521 ttcgtccgcg tctatcgcta ctcgtcgacc cggacgacct accagctccg cgtcagctac
     8581 tgagcccttc ccgcgcccgc ccccttccgg gggcgggcgc gccgttcccc tcgtctcctg
     8641 tagtctctcg ccgatgaaga tcgtcctgac gatgaacctg ccctggttcc ccgccgtcgg
     8701 gggggccaac aagtgcaacc gcgtgctggc ggaggggctg gccgggctcg gccactcggt
     8761 gcgcgccgtg gtgcccgccc tcggtgtgcc gtcacggatc actctggagg agtggcggcg
     8821 gatgctcgcg gaccagggct tcgccagccg gcaggaggat ggcgtcgacg tcttctcctg
     8881 gaacggggtc gaggtccacg ccgtcgccga gccgtcgcgg ctgcgcgccg agctcatcga
     8941 gcggctgcgg ggcttccagc ccgattgggc cctggtctcg tcggaggacc cgtcgcagaa
     9001 cctgctcgac gccgcgctcc aggcgcaggc cgctcccatc gtctatctcg cccacactcc
     9061 ggccttcctg ccgttcgggc cccaggcgtt ctatcccagc gagcggcggg ggaggcggct
     9121 cggccaagtc gcggggatcg tcaccgtcag ccgcttcatg gccgagtaca tccgccgctg
     9181 gggcggcctc cacgccgacg tgtttcactt ttccgtctac ggcccggggc ctttccctga
     9241 tttcggatcg gagggctccg gcgaagggtt cgtcacgctg atcaatccct gtgccgtgaa
     9301 ggggatcgcg atcttcctcg ggctcgcccg ggagctcccc gaggtgcgct tcgcggcggt
     9361 gccgacctgg gggacgacca ccgccgaccg cgcggccctc gccgccgtcc ccaatgtcac
     9421 cctgctcaag ccggaggtgg acatcgaccg catcttcgcc cgcacccggg tcctgctcat
     9481 gccgtcgctc tggcaggagg cgttcggcgt caccgcggtc gaggccatgc tgcgtggctt
     9541 gccggtgttg gcgagcgacg tcggcggcct ccccgaggcg acgctgggtg ccggccgcgt
     9601 cctgccggtg cgccccatcg agcgcttcct cgaccgccag gacgacaacg aggtcaccaa
     9661 ggccgtggtc ccggaacagg acctgacgcc ctggcgggac gccctgcgcg agttgctcgg
     9721 cgaccgcggc ctctaccccc gcgagtcggc ccgggctcgc gaggcggccc atcgcttcaa
     9781 cgccgggctg ggcgccgggc cgttcgtcga gatcctcgac aggacaccgc gccacgctcc
     9841 ggaggccgcg gcggcggctc ccgcgccgga ggccgaggcg gggggcgtcg ccgagctgac
     9901 tccggagcag cgggccctgc tgatgctgcg cctgcgcaag aagacggctc agaagtaggc
     9961 cgggctcata ggccccctgc tacactggga catgttgtcc caacggcagt ccgttctcct
    10021 cgcggccttc ctcttcctcc ccctgaatcc agcgtccgcg cagcccgagc ccggagcctt
    10081 cgagctcctg gccaaggcgc ggcaggccta cgcccagctc ccttcctatc gcgaccacgg
    10141 cgagatcgag acgaccctct ccgcgggcgt cacggtccgg caccggttcg agctgaccgc
    10201 cgcgcccggc gccttccgtt tcctcctgga gcgggacctc ggcggcgagc cgcagcggcg
    10261 gatgatctgg cggagcggcg gcgaggcgtt ccacttcgac agctgcctcg gacagtacaa
    10321 gacggtcgcc tccctcaccg acgaggtggc gggcgcgctg ggcagcggcg gcgcggacgc
    10381 cctcgtcgtc ccgatcaccc tggtgggcgg cgccggcgcg ctggccgacc cggaggtcgc
    10441 caccgtcgag ggggaggagc cctgcggcga cggctcctgc tggattgtct tcttctcccg
    10501 cgccacggac gtggagtccc gtctctggat cgacagccgg agcttcctga tccggcaggt
    10561 ggaggtccgg cttcaggaca cgggggacgt cgtcgatcag gccatggccc aggccggcct
    10621 ggcggtccgg gggacggcgc cgggagccgc gcgggggacc atcaccatcc gcgtgcgcca
    10681 tgagatcgag gccggcaagc cggtccagcg ggccgaggcg gcgttcggac cgcctcccgg
    10741 ggcgcggcgg gtcaccgagt ggcaggtcgc gggcgacgac gagctgccgc aattcggcga
    10801 ggagatcacc gtcgccatcg ccgccgtgac cgtgcgggtg gtggacaatc acggccggcc
    10861 ggtcgagggg ctcgcccccg gggacttccg ggtgctggcc aaggggaagg agatccccgt
    10921 agtggcggtg gactgggtgt cgtcggcgag gaccgaggcc ccgctggacc cggacggctc
    10981 cgcggtctcc tccggcccct ctgcgccggc gccgtccggc gagcagccgt gcaagcgggt
    11041 cgtcttcttc gtccagtcgg gcaaccatcc cacccggctc aaggggcaga tgcggctgcg
    11101 gccccatacc cgcgagctcc tggagtcctt tccggcggcg gaccggctcg cggtggtctc
    11161 ctacgactcc cacctcaaac tgtggcagga cttcacggac gaccgcgagg cggtcttcca
    11221 ggccatcgat caggccatgc tgttcggcgg cgaccgccag accgtggtgc gtccgagccg
    11281 cgaccgctcg tcgctcgcgg cgcacctcga cttcgacgcc gcgctgcgca cggcctcgcc
    11341 ggagcgggcg ctccaggtca cggccgaggc cctccagccc ctgcccggcg agaaggtgat
    11401 gatctacgtc ggctgggggt tggggcggtt cggctccgac ggcgtccgca tgacccccga
    11461 ctaccggccg gccatcgagg cgctggacgc cgcgcgggtc accgtcttcg tgctcgacat
    11521 caccgacgcc gactaccact cgctggagac ggggctccaa tcggtcgcct accagacggg
    11581 gggctcctac gcgaagaccc acatcttccc ccagcacgcc accgacttcc tggccgggac
    11641 ggtctccggc cactacgtcc tgaccctcga ccgcaccgcc ctgccccctc agggatcgcg
    11701 ggtgcggatc catctgcgcg acaagaaggg gacggtctac tacgcccggc ccgacccggt
    11761 gcccggcgtc ggcgacaccg gacggtgaag agcctccagg cctccgcctc ggctgccggg
    11821 gtaacctttt ctcctcaaac cgcacctatg tagtgcgatg tcggcggatg cccgggattt
    11881 cgagagcctg ctccagcggt acgcgcggtt gatggcgagc gccgtccgga aggtctgtgg
    11941 ccgcgagcac cgtgacctgg tgccggatgt ggagcaggag atctacctcg ctctgtggaa
    12001 gcggctccag gatggaaaag agatccgcca ccccgtctcc tacctctaca aggtggcgct
    12061 gaccaccgcc ctcgccttcg tgcgccggcg gggaggcgag gcgaccacga acgatggcgg
    12121 cgagctggag aggcccccaa cgccgctccc cggcgccgac gggctgctgc cagccgagcg
    12181 ccggctcctc ctggaacagg tgttgcgcga gctgccgcgc gagcaggcgc gggcggtgcg
    12241 cgcctatctc gccgggttca atcaccagga ggtggcggcc ctgtacggct ggagcgagcc
    12301 ggtggcgcgg catcgcatct accgggggct cgagagcctg cggggcaagc tggtggagac
    12361 gagccgatga gcgacgagcc catggatgaa gccgagggcg ccgcctggcg cgaggcctac
    12421 cggggattcc aggcgccggg ctcctccggc tgtccggatg acgaggccct ggccgcgctc
    12481 gccaccggcg agacgaccgg tgccgagcgc gagcgcctcg ccgatcacgt cgtcggctgc
    12541 cggcggtgcg ccgagcgcta ccgcgtcctg cgtgacctcc accgggaggc cggctccgag
    12601 ccggagcatg aaggggccgg gagcggcggc aggggatcgc gcctccggct cctggccgcg
    12661 gcggcggcgc tgctgctggt cgcggggctc ggttggatcg tcggcctgcc gggcacgtct
    12721 ggcgtgctgg atccggaccg gctgcgcggc gagccggcga tggaggaggg ggtgcgtccg
    12781 gcgccggacg cccggctcgc cgcgccgccg cgggagctcg cctggccggc ccgccggggc
    12841 gcccactcct accgggtgat cctgtacgac gccacctctt cccctttgtg gacgagcgct
    12901 ccggtcacgg caccgatcct gaccctgccg gacgaggcgc ggtccaggct cgtggggggg
    12961 gactcctact tctggatcgt cgagaccggc cacgggccgg acgcgccggt ctcgcgcctg
    13021 gggccgttct ggttcagcct ggaggagcgc agggagcgtt gagctggcgc gcgtttctct
    13081 ccattcttgt cctcctcgcg gcggccgtgg cggcgagctt cgcgctgggc gcctcgcggg
    13141 agtctctccg ggagacgacc ggcgccgggc tgctggtcga ggaggtgggt gacggctcct
    13201 ccatggcgga ggccgggatc cgggcgggcg acatcctcct gtcctgggag cggcccgcgg
    13261 cgcatggcga gccgcccgcc cgtggtcccc tgcgctcctt tttcgatctc cagctcatgg
    13321 ggctggagca gtcctcgcgg gccaaggtgc gggtgacagg agcccggctg gggaggacgc
    13381 tcacctggac gctggccgag ggggaatggc ggatcaccgt ccgtcccctg atgccgccgc
    13441 ttctcgagtc gggatacgcg gcgggacggc ggcggctcgc ggcgggcgag gccgggcgcg
    13501 cattcgccca gtggaaccag accgcggaag cggcggcggc agcgggccag ccggtcctcg
    13561 ccgcgtggct ctggcggcag gtggcgtccg aggcgctcga gcggcaactc tgggacgagg
    13621 cggagcgggt gctggcccgt ggcctggccc tgccgctgcc ggctgccgtc cgggcgcggc
    13681 tgctcacctt ccaggcccag gccttcgacc gccaggggga gctgctccag gcggaggcgg
    13741 ctctgcgcgc cgccctcgcc gcgcaggcgg ggaccccgcc gggcctggcc ttggcggcgg
    13801 acctcgatga cctgggctgg gcgctgatcc ggcgcggcga cctcgacggc gccggcgccg
    13861 ccttccggcg ggcgctggcg ctgcggctcc gtctcgctcc cggcagcctg ctgctcgcgg
    13921 cgagctacaa caagctcggc atcctgcaca tggagcgtgg cgacctcgcc gcggccgagg
    13981 agatgttccg gcgcgtcgtc ggcctgtggg agtcccgggg gccgcacagc ctggccatgg
    14041 cggccgggct ggagaacctg gggctggtca ccacctaccg gggcgacctg gcggccgcgg
    14101 cgggcctgat gcggcgcgcc gcggcgctca agcagcgtct cgatccggac agcgagcacg
    14161 tcggccgcag cctgttgaac caggggatgc tccagtggcg gcgcggcgac ctcagctccg
    14221 cccaggatct cctcacctcc gccctcgggc tctggcgcca gctctccccc aggagccggg
    14281 acctcgccgg cgtgctgacc aacctggggc tcatcgctct ggaccgcggc gagctcggcc
    14341 tggccgagcg gcggttccgc gaggccctcg ccctctggcg cgagctcgag cccggcactc
    14401 cgacggtggc cgggacgctc aacaacctgg ccatggtggc ccggcggcag ggcgatccgg
    14461 tgggcgccga gaggctgctg cgcgaggcgc tggccctcga ggagaaggcc gcgccgcagg
    14521 ggctcgccgc cgccctctat ctgcagaacc tcggcgagct ggccgaagac cagggggcgc
    14581 tcgaggaggc ggagggcctg atgcgccgtg ccctgtccat cagctcgcgt ctcgcccccg
    14641 gctccgagct ggaggcgaag tcctggtatg gcctgggccg cctgcaccgc cgcgccggcc
    14701 ggccggccga ggcgcgggag gcctggatgc ggggggtggc ggcgctggag gagcaggagg
    14761 cgaagctcgg cggcaccgag gagatccgct cggcttggcg gggcaagcac gggggccttt
    14821 atcacgacct gctggagctc ctgctggagc aggggagggc ggacgaggcc ttccagctct
    14881 ccgagcgcta ccgggcccgg cggctccttg ccctgctcgc cgagcgcgac ctgctctggc
    14941 gcgaggacct ccccgccggc ctggcccggg agcggtcacg cctcgccgtc gagcacgacc
    15001 ggatccaggc ggcgctcgca cagctcgacg ccggcgccgc cgacgcgcgt gcccaggtgg
    15061 agctcctggg gcgccagctc cgggagctgc gcaaccgccg ggccgagctg gcggacgcga
    15121 tcctcgccgc ctcgccgcga ctcgcctctc tgcagtaccc ccggccgctg gacgcggccg
    15181 ccgctcaggc cgccctcgct ccgggcacca tcctcctcgc ctatgccgtc gggctgcggc
    15241 acagccatct gttcgtcctc gagagggacg gccccctccg ggtcttcctc ctccccgtgg
    15301 gcgaggacgg cctgcgggac cgcatcctcg gcttccggca cctcctgcgg cagccagccg
    15361 gcggaacacg ctcgcgcgcg gcgttggagg agcggggtca gacgctggcc cgggacctcc
    15421 tcgcgccggc cttgccccat atcgcccgcg ccgaccggat cctgatcctg gcggacggcc
    15481 ccctgcacgc actgccgttc gctcccctgc cggtggcggg ccgaccggcg gccagccgct
    15541 tcctggtgga gtggaagccg ctgcacacgg ccgcttcggc caccgtctac gccgagctgc
    15601 gcaagcggcg ccggccgtct ggctctcgag gggcttcccg cgccgggctg gtcatcttcg
    15661 gcgacccccg caacgcacgc gacctggacg gccgccccct gccgccgctg cccgccaccc
    15721 gcgccgagag ccgggcgctg gaacggctct ttcccggcgc ccgcgtcttc ctcgggggcg
    15781 acgccacgga ggaggcgctc aaggccgccg gtcgctccgc ccgctatctg cacgtggcga
    15841 gtcacgccgt gacggacgag cacctccctc tggattcgta cctggcgctg tcctcccctc
    15901 ggcggccgcg gcccgggcag gacaacggcc tcctccaggc ctgggagatc ttcgagcagg
    15961 tccgcctcga ctccgacctg gtgaccctcg cgtcgtgcag cacggcgttg ggccgcgagc
    16021 tggccggcga gggggtcctc agcctggccc gggccttcca gtacgccggt gcgcgctcgg
    16081 tggtggcctc cctctgggac gtcccggacc gctccacatc gctgctgatg cagcgcttct
    16141 atgccgggct cgcgcggggg ctcgccaagg acgaggccct gcgccgcgcc caggttgccc
    16201 tgctgaaacg tccggagacc tcccacccct tccactgggc agcccaccaa ctcgccggcg
    16261 actggcacta gcggccggaa cggcggcccg ggcctcgcct gccgggcgtg ggaaaaaacc
    16321 gcccctccct ctctcttcct catcagacgc ggcaacgtgc cgcccacaac cttagtggag
    16381 gagaggaaat gagaatcgcc gtattgatcc gcaaactttc gaccgccggc gccctgaccc
    16441 tgctggccgc caccgcggcc cacgccgcgc cgctcgactt caaggacgtc atggccgtgg
    16501 ccagcaccag ggatttcctg aagatcgacg acctgcaggt cgtggtgagc cgcccgccgt
    16561 tcgacgcctg cgtggactgg aacctcgctc tctgtatcga ggaggtctgg cacgagtcgt
    16621 gggtcgtctg ccatcagccc tggggacagg aaaagctcgt cgctctctcc tgcggcgcac
    16681 tgaggaccgt gaccaagcgc tgcgccgacc gcacgtggca cgaggacgtg gactacgcca
    16741 cgggcggccg ctactgcgtc gagagcccgg agcaccagta cctttgcgac aacgggcccg
    16801 ggatcgtgca gtcacaggat gtccggatct cctccaggcc ctgcgcctag ccggcggcca
    16861 ggggggcgcc gccgcgcggc agaggcggcg ccccgctccg aacgagtcca tcgattgtca
    16921 aagaacctcc gtggcctctc gcgcacggtc cgacccgagt cagagccctt tcgaccctcc
    16981 tcggagcgcc cgcgaccctg gggtcaagag gctcgacccg agggtcgaga ggctcgaccc
    17041 tgctccgaac ggtcgcgacc ccagggtcga gaggcttgac cctgctccga acggttgcga
    17101 cccgagggtc gagagactcg accctcgggt cggatcgaag tcaggcaggc gcgtccattc
    17161 gccaagagat cgtatcatac cattaagatc aggatcggcc ttgtctccag gtaatactct
    17221 gtgtagagtg actggcggcg gtccaccgac tcgtttctgg ccggcgaccg ctggcaggat
    17281 gttccttgac aactgaatag tacgcaggcg ccgccgtgga ggatctgggg ggcactatga
    17341 gcagcagaat tcggcgtctc tccgccctcc tctctgtcat gctgtccccc ccaaaccccg
    17401 acgggccgtg tctcgtataa ggagagagga gggaagatga gaaagagcta cgaagataga
    17461 caaccggtca tgaagaggag ggtccacgcg tccatcgtcc tcctccttgt ggtctcgctc
    17521 ctcgggatgc ctatggccag catggcccag aagcgcggca gggacgccaa gcagctcggc
    17581 cgccacttgc ggcagggaga gggggtgacg caggccatcc agcaggtcgt cgggccgatg
    17641 cccggtatcg ccatcgctcc gtttctcggg ctcgcggctc tctccgggct ggccctgctc
    17701 gcggacaccg agtacgcgag gacgtccgac aacacattcg tgcgggccgt ctgtgacaac
    17761 agcttggtcc gggaggccca gaagtacagc agcctgacgc tcttccttct cctctgcggg
    17821 ttggcgctcc tcacctatct cgccagcagc ggcaagatcc gggggatcgt cggcaagatg
    17881 ctgcgcgcaa cggaggatgg agtggtcacg cccacctacg cgctcctctc cctgggcgcc
    17941 ttctccggtc tttccatggt ggcccagccg gccgtcgccg agatggggtt cagcctgccc
    18001 tcggccgtcc tcctcgcctt cggagtcgcg ttcggtctcg ccgcgatgat gaccgtccgc
    18061 ttcgccttcg atgttctgat ctggttgagc ccggtgccat tcatcgattt cctgttcgag
    18121 acggccaaga acgtcttctc cgtggggctt ctcgtcctct atctcttctt tccgggcttc
    18181 gccaccgcgg tggcggtgct cttcctggcg gtgtcgctcc tcctgtacgg ctgggccatc
    18241 cggatcgtcg gcttcgcctt ccagatcgtg ctccggccgg tcttggcccg gctcgtgccg
    18301 tcgctccgga cgcggctgct cgacgagaag cgcgcgggcc acgcgggtgt ggcgggtgcc
    18361 cggatcgcca cgccggcggc ggcgctggcc gtccccggct tcagcaagcg ccaactcggg
    18421 actctgttcc tgacgacgga cggcctgagc ttcagcgccc gcccccttct cggccgtacc
    18481 cggcagcagc cgctccttct gaggccggac acccggccgg tcctctgccg ctgcctcttc
    18541 tggtacgagg tccgggtcac ctctcccgag ggacgcctct acaagctcgc cctgccgaag
    18601 accctgcaac ccgagtggac cgccctccag acgctcctcg ccgccgagga cgccggagcg
    18661 atcggaggcg ccgacaagct ccggaagcta gccgaccgaa tgggagagca gttaaaaggg
    18721 atgccgccga cggcccaggc caggtaggta aaggagggca ggcgatcccc aagctgggat
    18781 ggggtacacc ggcctatgcg cttcatccac cgaactgatc caggagcgcc gccaaggtag
    18841 tcagcgcgag tgatgaacga aaccgttcgt tggatcagct ccagtgggcg gactgggacc
    18901 gcgatggaaa cctgctggtg gccacgcgga gcgggaagct cgaaatctgg aatctcgccc
    18961 gagaccgcac ggaggtcctg ttcgaagagg acctgtctct tttggcgccg aatcctgttc
    19021 ccgcgccgga ctgggcgcgg caggggtaag aaaccgctga cctggcgcgc gccgatcacc
    19081 gctttctcgc tgacgagccc tgcccccttg gggcctcctc gggcaccctt tcttgtagga
    19141 cggatcttca tgactcgaga aaggagtcct catgaaccgc tccctgcttc cccttgctgt
    19201 cctcctcggt gccgctctct tttcgggacc aacctatgcg gccacggtga acgtgagcgc
    19261 gctctggtgc ggctttggct gtagtgaagg acacaacgac acgcagtgca tctgtaccta
    19321 cgacagcaac tacagcccga gcagcccgtg ggcctggatc agttgcgagg aggtgcgctg
    19381 ctgcgacacc gagtacgcct actgcgatct cggctacgtc ttcgaggggt acgacgggac
    19441 ctgctcttgg cggcccggta ccgagacctg cgccacgccg acgccgaacg gcggctggtc
    19501 caactgcgag gtccagtgcg gctcgagcgg ctgctgggcg ccctatcggg tcgctcactg
    19561 ggaaactgtc aacgagggaa gccccgatta ctgcacgcgc tcgaacgaga ccggggactg
    19621 gtggaatccc tggacctgga cgacgccctg cactctcgag gaccagtctc cctgggacga
    19681 ccctgggagt ggcctggtct gcggctgatc cgactggggt attcaccggc aggcgccggc
    19741 gtttctctct ccggcttgcc agcggccggc gggtgcgctt agattcaccg cgatgccggt
    19801 ccgtacccag tcgccgccgg gcgagaggcg ccccctgtcg tccatcctcg ccctccgccg
    19861 cgatcctctg ggctacctgg agggtctcgc cgcccgctat ggcgacatcg cgaccatcca
    19921 ggtcgggtcc tacaggctct tcttcctgaa ccatcccgac ctgatccacg aggccctggc
    19981 cacgagggac gcgagcctcg tcaaggggcg catcctcgac caggcgcggg acgtcttcgg
    20041 ccaggggctg ctgaccagcg agggggactt ccaccgcaag cagcggcggc tgatccagcc
    20101 ggcgttccac cggcggcgga tcgaggccta tgccgatgcc atggcggccg acgccgccgc
    20161 ggcggccgcc cggtggcggc acggcgagac cgtggacgtg gccaccgaga tgatgcgcct
    20221 gactctgcgg atcgtggcgc gctgcctgtt cagcgtggac gtggaggacg acgaggcgcg
    20281 ggaagtcggc ggcgctctcg atgcactgat ggccgccttc agcacgaccg tcctggggag
    20341 cggcttcatc cgcaagctgc cgcttccggc gttccgccgg gcccggcgcg gggtgaagac
    20401 cctggacgcc ctggtctatc gcatgatcgc ggagcggcgg cggacgggag gcggcggcgg
    20461 ggaggacctc ctctccctgc tgatggcggc ccgcgaggag ggggagggga gcgggatgga
    20521 cgaccgccag gtgcgcgacg aggtcctgac cctgttcctc gccggccacg agaccacggc
    20581 caacgcgctg gcctggtcct ggttcctcct cgcaaaccat cccgaggccg aggcgagctt
    20641 ccacgcggag atcgactccg tccttggcga ccgccttccc ggagcggcgg acctgccgtc
    20701 cctcccatac aaccgcaagg tcttcaccga gtccatgcgc ctctatccgc ccgcctgggc
    20761 gatctcgcgc cgtgccgccg agccgctcac cctcggaggg tacccggtcg ccgccggctc
    20821 cggggtcctc gcctcgcaat gggtcatgca ccgcgacccg cgctacttcg aggacccgct
    20881 ggccttccgg ccggagcgct ggaccgagga gttcgagtcg cgcctcccca agctcgccta
    20941 cttccccttc ggcggcggac cgaggacctg catcggcatg ggcttcgcgc ggatggaggg
    21001 cgtgctcctc ctcgccaccc tcggccgccg ctggcggatg cgcctcgtcc ccggccagcg
    21061 gatcgagccc tccccccgca tcaccctccg cccgaagaac ggcattcggg tcaccctgga
    21121 gcggcggggc tgacctgggg gagagggagc catcgggcct cgcgctcgat ggtgcggatg
    21181 aatcggccgc cctgctgccg cagcttgccc cactcggcgc gggtgtagat caggatctcc
    21241 gcgggaaccg gcagggggag caggtcccag tccagggcgc ggcgctcgaa cggcttgccg
    21301 gactcctcga cgacggcgat gaggtccagg tcgctcccca ctccggcgtc gccgcgagcg
    21361 tacgagccga accagccgag gcggaggagc cctgcatgac gcggtgcctc ccgcccggcc
    21421 cacgctctca gcgcctcctc gacctcggcg ggtctaggcc atctgagtac gcgcgaatgc
    21481 aaggatctca ccggcataat tgatcgcgtc ccggctctgg atctctccgt agtgctcgaa
    21541 gggtgggcct tccgcgtggc cgtcaggata ccgggttccg atatagaagt tgtccaggac
    21601 cttggccttt tgtaccagca ggggatccac ctcgatggga agctcctcca ggagacgagc
    21661 cacgacatgg ccccaagcct cctggaagag gtggaggttg agcgccttca ccgccttctc
    21721 ggccgcctgc tgcgccgcga agcaggccca ttcgtggtgc ccctgctgac gtgacgcctc
    21781 cgcctgagcg aggtcccgct ccgcctgcgc gagccaatcc cgggaacgat tcatcccatc
    21841 tcctccgcct caagcctagc gcaccccaca gtttttgtgg gtgaatcggg ctcaacctgc
    21901 taacatgcac aatgttctgt gcaggggcag gaaggagact ctattgaggc gacattcccg
    21961 ggtcacaccc atccgcaatg gtctggccct gctggtcgtg ctggtctccc ttctgaccct
    22021 cccgggctgc cgagaagcgg agcctcccgc gccgccccaa ccctccaagc cgcgcctgtt
    22081 cttcctcggc gtcgacggcg tctcctggac cgtcctgggg ccgatgctcg agcggggaga
    22141 gctgccggca ttccagcggc tgatcgagga ggggacctcc ctgccccggt tcgacaccct
    22201 gcagatcacc cactcgccgg tcatctggac caccgtggcc acgggccggc ggccggcggt
    22261 ccatggcatc cgcgacttca cggccgagct gcccaacggc cggaagatcc cggtcaccag
    22321 caacatgcgc aaggcgcggg cgatctggga ggtggccagc cgccgcggcg tgagcgcggg
    22381 ggtcatcggc tggtgggcca cctggccggc cgaggagatc ctcggctacg tggtgagcga
    22441 tcacgccaat ccggccttcg gcgacgtgat gaccgccgag gggcgcttct ggaccgccga
    22501 ccgcgagagg ctggcccagc tccgccggca ggtctatccg gaggagatcg cgccggtcct
    22561 cgcccgttat tggatcaccg gcgagacctt cccctgggac gaccttcagc ggcgcggccg
    22621 cttcaccgac gagcagatgc ggctcgcccg cgccgccgcc tgggccgagc cggacctcta
    22681 ctcctggctc aagaccttct accgggtcga ctatcccctc ttccgggccg ccctcgacct
    22741 catgaaggag cgccccacgg acctccagat gctctacctg cgcggtccgg acccggtgca
    22801 gcactacggg tgggacctgg tggagcccga acggtacgcg cgcaagccgc cccacctgga
    22861 gcgcgaccgg acggtggtgg agggggtcta tcgctacgtc gacagcttcc tgggggagat
    22921 cctcgacgcc ctgccgccgg acgcctggct gatcgtcgcc tcggaccatg gcgccgagcc
    22981 gtcggacgat gccgcggacc cggcacggac ctcccgcccg ggcgagcaca gcgtggaggc
    23041 caagggggtg ctcttcctcc gcggccccgg ggtgcgccgg ggagccaacc tggagaaggc
    23101 cacccccttc gacctgatgc ccaccatggc ctggctcctg gagctgcccc tctcctccac
    23161 cctgccgggg aggatcctca ccgaggcgtt cgaggaggac ttcgcccgct ccctgccggt
    23221 ccacaaggtc gcccgctacg gcccgcgccc taccgggccg ctcctcccct cgccggagga
    23281 cgaggagatg ctgcgcagcc tcaaaaattt gggctacatc cagtaagttg aatcggcccc
    23341 tccgctctcc gggcactgtg actttcctgt aacatccccg tagcgggaag ggcacacccg
    23401 gcgccaagac tgggggccgg gcagtcgggg gctggctccc gtgtgccgtc gccgcgccat
    23461 ccgtacatct gagcttccag ggatcgctgc cgctcgtcgt ctgtctcgcg ccagcgctcc
    23521 cgccgggtgg cattgcgttc ttacatcaac tctccaaagg gaggaggacc tatgttccag
    23581 cgatcgaggc cgatgctgct cgccctgctg ctcatggtgg gagcggtggc gggtgtcttc
    23641 gcgcagacca gctcgacgac cggcgagatc tccggccgcg tcgtcgacga gaccggtgga
    23701 gtcatgccgg gcgtgaccgt caccgccacc aacacccaga ccgggctcag ccgcaccgcc
    23761 gtcaccgaag aggacggcga ctacagcctc gggctcctgc ctccgggcat ctaccgcgtc
    23821 gccgccgagc tgtcgggctt ccggtccgac gtgcgcgaga acgtcaccgt gctcctcggc
    23881 gtgtccagca acgtccgctt cactctgtcg gccgagctct ccgaggagat caccgtcacc
    23941 agccaggctc ctctggtgga cgtcaccgac gccgacctga cgctgtcggt cacccaggac
    24001 cagatcgaga acctgccgat cctgggccgc gacttcagcg acctggtcct gctcaccccc
    24061 ggcgccggcg aggcgttcgg cgaccgcgtc gccctgaacg
//
LOCUS       DASPJI010000640         5763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_338793, whole genome shotgun sequence.
ACCESSION   DASPJI010000640 DASPJI010000000
VERSION     DASPJI010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338793"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>5763
                     /locus_tag="VEL74_18095"
     CDS             <1..>5763
                     /locus_tag="VEL74_18095"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64496.1"
                     /translation="LSRSPFFDVLLVLQNTPPRALELPGLALLPAPGGGGAALFDWTF
                     NAVETGGALALSLAYSADLFEAPTAERALSHLASLVREAGAAPARPLGDLPLLSPAER
                     FQLAREWNDTAVSRPGGLFLHGLVADQAARTPDAVAVTFEGESLTFGELHLQARRLAR
                     SLRVHGCGPEVRVGIAMERSLELVVALLGTLEAGAAYVPLDPDHPRERLALRLEDAAP
                     AVIVAQERLLPRLPEAAIPVVLLDGALSGRPLAAADSETGSGTRCGDLQLAYVIFTSG
                     STGRPKASMLHHRGIVNRLLWMRQAYGVGPSDVVLQKTPFGFDVSVWELFEPLVTGGR
                     LVMARPEGHRDGAYLADLVGREQVSVLHFVPSMLRLFLEEPDLSACGSVRLVVASGEA
                     LGPDLARRFRERLAAPALENLYGPTEASVEVTAWSCGEADLAAVPIGRPIDNAGIHLL
                     DRGWQPVPIGVPGELWIGGVGVARGYLGRPGLTAERFVPDPFSSAPGARVYRSGDLAR
                     HRADGAVEFLGRVDQQVKVRGFRVEPGEVEAALRDHPAVRQAAVVARHGSGGPALVAY
                     VVSREGAGGSTEPAALRTFLRQRLPEPMVPSLFVRLPALPLNASGKLDRRALPEPAGE
                     GGDVGAAAPVAPRDPVEEGIAAIWREVLGTGRVGVHDVFFELGGHSLAATRVVSRVRA
                     AFGVDLPLRALFEAPTVAGLGERVRLALRHGAGTALSPTSPPLAADPAGALGAPLSFA
                     QQRLWFLHRLEPGSTVYNLRVAVRLQGDLDPGMLARAFGEVVGRHATLRTHFAEVEGE
                     PVQVIEPAVPPAAFVLPLADMTALGERSRDEALRLAEELGGRPFDLGRGPLVRPLLLR
                     LPGGAGSAPLSALLVVMHHVVSDAWSMGILVRELSALHDAFRRGAPSPLPELPVQYAD
                     FARWQRRWLSGEVLAGELAWWRERLAGLPARLDLPTDRPRPPVPSRRGAHRDLRIAPE
                     LGARVAALALREGATPFMVLLAVYQGLLARWSGQDDFAVGTPVAGRTRVEVEELIGFF
                     INNLVLRARLGGEPDFHELLGRVRSTTLDAYGHQDLPFEKLVEELAPERSLAHSPIFQ
                     VVFGLQTAPGGTEEAERTGGSGDGLDTPELGSRTAKYDLLLSLFHAGAGFEGLAEYDT
                     DLFDGTTVDRFLNQLETLLAAAVTDPGRPLADVPLLAAAERHQTLVEWNGAVTPYPRD
                     ATVHQLFEEQADRDPGAVAVVSPAGTMSYGELEARANRLAHSLRAWGVGPEMLVGLCL
                     ERSAEMVVAMLAILKAGGAYVPLDPAYPLERLAFLLGNAFMPVVLTEERVADRLPAGP
                     MTRLVLLDADRDELAAWSESRPEPWALPENLVYVMYTSGSTGEPKGVAAIHRGVTRLV
                     RETGYARFGRDEVFLQLAPVSFDASTLEIWGPLLNGGRLVVPEPGTVSLAELGGLLAD
                     QGVTTLWLTAGLFHEMADAHLAGLSPLRQLLAGGDVLSPAHVRRVLEDLPGRRVINGY
                     GPTENTTFTCCHPMADPADVGPSVAIGRPIANTSVYLIDRRTGDRPVPPGVAGELAIG
                     GDGLARGYLDRPDLTAERWRPDAASGRSGERLYQSGDLARYLADGRIEFLGRIDQQVK
                     IRGFRIEPGEIEAALTRHPAVRESVVVAREDRPGDRRLVAYVATDTLGGDEGERELRS
                     FLAEQLPAHMVPAHVVFLEALPLTPNGKVDRRALPVPGTEGVPAAGFVAPGDRVEEGL
                     AAIWSEVLGRSPIGARDNFFELGGHSLLAVRVISQVRDRFGVELPLRVLFEEPTVASL
                     ARSLREAQANEDAPLSEAPPLRPLLRNPRPEHEEVFPLSFAQQRLWFLDRLEPGSALY
                     NMPMVVRLEGALDAALLEGAVGEIIRRHETLRTTLRMGAAGGEPVQAVAPPGPFVLPL
                     ADLSGLAGGITEEVRRLVEED"
BASE COUNT          635 a         2181 c         2124 g          823 t
ORIGIN      
        1 ctgtcccgct ccccgttctt cgacgtcctg ctggtgctcc agaacacgcc gccgcgcgcc
       61 ctggagctgc ccggcctcgc cctcctgccg gcaccgggcg gcggcggggc cgccctcttc
      121 gactggacct tcaacgcggt ggagaccggc ggcgcgctcg ccctctccct ggcctacagc
      181 gccgacctgt tcgaggcccc caccgccgag cgggccctgt cccatctggc gagcctggtg
      241 cgggaggcgg gcgccgcccc ggcgaggccg ctgggggacc tgccgctcct ctccccggcc
      301 gaacggttcc aactcgcccg ggagtggaac gacacggccg tgtcccggcc cggcggcctg
      361 ttcctgcacg gcctcgtggc ggaccaggcg gcgcggacac ccgacgcggt ggccgtgacg
      421 ttcgaagggg agagcctcac cttcggcgag ctccacctcc aggcccgccg cctggcccgc
      481 agcctgcgcg tccacggctg cggtcccgag gtccgggtgg gcatcgccat ggagcgctcg
      541 ctggagctgg tggtggccct gctggggacc ctggaggcgg gcgccgccta cgtccccctg
      601 gaccccgacc acccccggga gcgactggcc ctgaggctcg aggacgccgc gccggccgtg
      661 atcgtcgccc aggagcgcct gctgccgcgg ctccccgagg ccgccatccc ggtcgtcctc
      721 ctggatggcg ccctgagcgg gcggccgctg gccgcggccg actctgagac cgggtccggg
      781 acacgatgcg gcgatctgca gctcgcctac gtcatcttca cctcgggctc cacggggagg
      841 cccaaggcct ccatgctgca ccaccggggc atcgtcaacc ggctgctctg gatgcggcag
      901 gcctacggtg tcggcccgtc cgacgtggtc ctccagaaga cccccttcgg cttcgacgtc
      961 tcggtctggg agctgttcga gccgctggtc accggcggcc ggctggtcat ggcccggccc
     1021 gaggggcacc gggacggcgc ctatctggcg gacctggtcg ggcgcgagca ggtgagcgtc
     1081 ctacacttcg tcccctccat gctgcggctg ttcctggagg agccggacct ctccgcctgc
     1141 ggctccgtcc gcctggtggt ggcgagcggc gaggccctcg gcccggacct ggcgcggcga
     1201 ttccgcgagc ggctcgccgc gccggcgctg gagaacctct atggccccac cgaggcctcc
     1261 gtcgaggtca ccgcctggag ctgcggcgag gccgacctcg cggcggtccc catcggccgg
     1321 ccgatcgaca acgccggcat ccatctcctc gaccgcggct ggcagccggt gccgatcggc
     1381 gtcccgggcg agctctggat cggcggcgtg ggcgtggccc ggggttatct cggccgtccc
     1441 gggctgaccg ccgagcgctt cgtccccgac cccttctcgt ccgccccggg ggcccgcgtc
     1501 tatcgctcgg gagacctggc gcggcaccgg gccgatgggg ccgtggagtt cctcggtcgc
     1561 gtcgaccagc aggtgaaggt ccgcggcttc cgggtggagc ccggcgaggt ggaggcggcg
     1621 ctgcgcgatc accccgccgt ccgccaggcc gcggtggtgg cgcgccacgg cagcggcgga
     1681 ccggcgctgg tggcctacgt ggtgtcccga gagggggcgg gcggctcgac ggagccggcc
     1741 gctctgcgaa ccttcctgcg gcagcgcctg cccgagccca tggtgccgtc cctcttcgtg
     1801 cgcctcccgg cgctgccgct caacgccagc ggcaagctgg accggcgggc ccttcccgag
     1861 cccgcgggag aaggggggga tgttggagcc gcggcaccgg tggcgccgcg ggaccctgtg
     1921 gaagagggga tcgccgccat ctggcgggag gtcctgggga ccggacgggt gggcgtccac
     1981 gacgtcttct tcgagctcgg cggccactcc ctggcggcca cccgcgtggt ctcccgcgtc
     2041 cgcgccgcct tcggcgtcga cctgccgctg cgggccctct tcgaggcccc caccgtggcc
     2101 ggcctggggg agcgggtccg cctcgcgcta cggcacggcg ccggcaccgc actgtcgccg
     2161 acatcgccgc cgctggcggc cgatcccgcc ggcgctcttg gcgcgcctct ctccttcgcc
     2221 cagcagcgcc tctggttcct ccatcgcctg gagccgggtt ccacggtcta caacctgcgg
     2281 gtggcggtgc ggctccaggg ggacctggat cctgggatgc tcgcccgcgc cttcggtgag
     2341 gtggtgggcc gccacgcgac gctccgcacc cacttcgccg aagtggaggg ggagcccgtg
     2401 caggtcatcg agccggcggt gccgcctgcg gccttcgtcc tgcccctggc cgacatgact
     2461 gcactggggg agcgctcccg cgacgaagcc ctgcggctgg cggaggagct gggcggccgg
     2521 ccgttcgacc tgggccgcgg cccgctggtc cgccccctgc tgctgcgcct tcccggcggc
     2581 gccggaagcg cgcccctctc cgcgctgctg gtggtgatgc accacgtggt gagcgacgcc
     2641 tggtccatgg ggatcctggt gcgggagctg tcggcgctcc acgacgcgtt ccggcgcggc
     2701 gccccgtcgc cgctgcccga gctgccggtc cagtacgccg atttcgcccg ctggcaacgg
     2761 cgctggctct ccggcgaggt gctggccgga gagctggcct ggtggcgcga gcgcctggcc
     2821 ggcttgccag cccgtctcga cctgcccacg gaccggccgc ggccgccggt gccgtcgcgg
     2881 cgcggcgccc atcgcgacct gcggatcgcg ccggagctgg gagcccgggt ggcggcgctg
     2941 gcgctccgcg agggggccac ccccttcatg gtcctgctcg ccgtctatca ggggctcctc
     3001 gcccgctggt ccgggcagga cgacttcgcc gtcggcaccc ccgtcgccgg ccgcacccgg
     3061 gtcgaggtgg aggagctgat cggcttcttc atcaacaacc tggtgctgcg ggcgcgcctg
     3121 ggaggggagc cggatttcca cgagctgctg ggccgggtgc ggagcacgac cttggatgcc
     3181 tacggccatc aggacctgcc gttcgagaag ctggtggagg agctggcgcc ggagcgcagc
     3241 ctcgcccact cgcccatctt ccaggtggtc ttcggcctgc agaccgctcc cggtgggacc
     3301 gaggaggcgg agaggaccgg cggcagcggc gacggcctgg ataccccgga gctggggagc
     3361 cggaccgcca agtacgacct cctcctctcc ctcttccacg ccggcgccgg cttcgagggg
     3421 ttggccgagt acgacaccga cctgttcgac ggcaccaccg tcgaccggtt cctgaaccag
     3481 ctcgagacgc tgctcgccgc cgccgtgacc gaccccgggc ggccgctggc ggacgtcccc
     3541 cttctcgcgg cggcggagag gcaccagacg ctcgtcgaat ggaacggcgc ggtcacccca
     3601 tacccgcggg acgccaccgt ccaccagctc ttcgaggagc aggccgaccg cgacccgggc
     3661 gccgtcgccg tggtctcgcc ggcgggcacg atgagctatg gcgagctgga ggcccgcgcc
     3721 aaccgcctcg cccactccct gcgggcctgg ggtgtggggc ccgagatgct ggtggggctc
     3781 tgcctcgagc gctcggcgga gatggtggtc gccatgctgg cgatcctcaa ggcgggcggc
     3841 gcctacgtgc cgctggaccc cgcctacccg ctggagcgtc tcgccttcct gctgggcaac
     3901 gccttcatgc cggtggtgct gaccgaggag cgggtggccg accgcctgcc ggccggaccg
     3961 atgacccggc tggtgctcct ggacgccgac cgcgacgagc tcgcggcgtg gagcgagtcc
     4021 cggcccgagc cgtgggccct gccggagaac ctggtctacg tgatgtacac ctcgggctcc
     4081 acgggcgagc cgaagggggt agccgccatc catcgcggcg tgacccggct ggtgcgcgag
     4141 acgggatacg cccgcttcgg ccgcgacgag gtcttcctgc agctcgcccc ggtctccttc
     4201 gacgcctcga cgctggagat ctgggggccg ctcctcaacg gcggccggct ggtggtgccc
     4261 gagccgggca ccgtctcgct ggcggagttg ggcggcctgc tggcggacca gggcgtcacc
     4321 acgctctggc tcaccgccgg cctgttccac gagatggccg acgcgcacct cgcgggcctc
     4381 tctccgctgc gccagctcct ggcgggcggc gatgtgctgt ccccggccca cgtgcgccgg
     4441 gtcctggaag acctccccgg ccgccgggtg atcaacggct acggcccgac cgagaacacc
     4501 accttcacct gctgccaccc gatggcggac ccggccgacg tcgggccgtc cgtcgccatc
     4561 ggccggccga tcgccaacac ctccgtctac ctgatcgacc gccgtactgg cgatcggccg
     4621 gtgccgccgg gtgtcgccgg cgagctggcg atcggcggcg acggcctcgc ccggggctat
     4681 ctcgaccgcc cggacctcac cgccgagcgc tggcgccccg acgccgccag cggccggtcg
     4741 ggagagcgcc tctaccagag cggcgacctg gcccggtacc tcgccgacgg ccggatcgag
     4801 ttcctgggcc gtatcgatca gcaggtgaag atccgcggct tccgaatcga gccgggggag
     4861 atcgaggccg ccctgacccg ccatccggcg gtgcgcgaaa gcgtggtggt ggcgcgggag
     4921 gaccggccgg gggaccgccg gctggtggcc tatgtggcga cggacaccct gggcggcgac
     4981 gagggggagc gcgagctacg ctccttcctg gccgaacagc tgccggccca catggtgccg
     5041 gcccacgtcg tcttcctgga ggcgctgccc ctcaccccca acggcaaggt ggaccggcgg
     5101 gcgttgcccg tcccaggaac ggagggggtg ccggcggccg ggttcgtcgc gccgggcgac
     5161 agagtggagg aggggctggc ggcgatctgg tccgaggtcc tcggacgctc accgatcggc
     5221 gcgcgggaca acttcttcga gctgggtggc cattcgctgc tggccgtgcg ggtgatctcc
     5281 caggtgcgcg accgcttcgg cgtggagctc cccctgcgcg tccttttcga ggagccgacg
     5341 gtggcgagcc tcgcccgctc cctgcgcgag gcgcaggcga acgaggacgc ccccctgtcc
     5401 gaggcgccgc ccctgcgccc cctgctgcgc aacccccggc cggagcacga ggaggtcttc
     5461 ccgctctcct tcgcgcagca acggctctgg ttcctcgatc gtctggagcc ggggtcggcc
     5521 ctctacaaca tgcccatggt ggtgcgcctc gagggggccc tggacgccgc cctcctggaa
     5581 ggggccgtcg gcgagatcat ccgccgccac gagaccctgc gcaccacgtt gcgcatgggg
     5641 gccgccggcg gggagccggt ccaggccgtg gcgccgccgg gccccttcgt cctgccgctg
     5701 gccgacctct cggggctcgc gggcgggatc accgaggagg tgcggcgcct ggtggaggag
     5761 gac
//
LOCUS       DASPJI010000641         4546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_338840, whole genome shotgun sequence.
ACCESSION   DASPJI010000641 DASPJI010000000
VERSION     DASPJI010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338840"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VEL74_18100"
     CDS             <1..511
                     /locus_tag="VEL74_18100"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HYG64497.1"
                     /translation="ANEGARAGAYRYKTRSFGLTILLPMEEQPNPFLDEVAQHSTFFS
                     RLHQFIRLSHAYIVVDGGIGTTLEAMMVWQLLQVRMLHDRQVVFVGPMWKGLREWIEH
                     DIIGRGLASPPDINIPVWVDRLEDALKVVGEARDEFLRRHKVVLPPVVDEETGDLQKS
                     PAPSPAAER"
     gene            complement(518..1069)
                     /locus_tag="VEL74_18105"
     CDS             complement(518..1069)
                     /locus_tag="VEL74_18105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64498.1"
                     /translation="MNTSNVHRALERLVDRLTKLEIPYAIVGALSLNAYGYQRTTSDV
                     DVLLRREGLESFKQACLGRGYLEKFPGSKGVRDTAQNVTIDVVLTGDYPGDGKAKPVA
                     FPDPARAAVRGERFALLPLPTLVELKLASGMTAPHRLRDLADVIELIRVNALQEDLAE
                     SLDPYVQEKYRELWRAAQLTDEE"
     gene            1439..2179
                     /locus_tag="VEL74_18110"
     CDS             1439..2179
                     /locus_tag="VEL74_18110"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HYG64499.1"
                     /translation="MLLQVGPVPAAGEEGPGILEFFWGAGPMAKIVLLTLVLFSLFSW
                     AVMIGKSVQLRRADAQSEKFMEVFHRSQRFSEVNAQAGKLRTSPLVGIFQAGYAEIDG
                     QIKAAAEADRPADRKAGAPGGYRITSLPSLERTLARAAGVELRGLQRWVPFLATTASA
                     TPFIGLFGTVWGIMMAFNQIGLSGSTSIVAVAPGIAEALINTAAGLGAAIPALIGYNY
                     FAQRMRQVKARMDDFILEFLNLAERNFT"
     gene            2189..2632
                     /gene="tolR"
                     /locus_tag="VEL74_18115"
     CDS             2189..2632
                     /gene="tolR"
                     /locus_tag="VEL74_18115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HYG64500.1"
                     /translation="MAFNVGNGNGDEESVLADINVTPLVDVMLVLLIIFMIAAPMLHQ
                     GIEVALPRAEAQNLPMRMEDPLVVSVNRDGQIFLREEPVEKDSLVEVLQAEISSRGGE
                     DDTVFLKGDRELPYGDMIGILDLLHQGGITRIGMVTERPQREGRR"
     gene            2636..3577
                     /locus_tag="VEL74_18120"
     CDS             2636..3577
                     /locus_tag="VEL74_18120"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HYG64501.1"
                     /translation="MSRGVNQVLAQRAKGEGRRPAAMSLGAALLLHALLVGAALGLPR
                     LQPPPPPLEFVPVTVLPAQALGVRRPAPARSKPAPPKPAPVQEAEPAAPEPEPEPEAP
                     APEPEPEPEPPAPKPREEAPVLPRETPKRPEKKPEPKPERTPDRTPAPKPAETGRKPA
                     DSKKPSSETGRDTKVPTPKPAEPRPSQGTQPGGTGDQVGRQGAATGSPTGTTAFGSEI
                     AGLDNPDFTYGYYIDRLLSLIDANWVRPNMGSGVRAIIAFRIQKDGSITELRVAESSG
                     YNAFDLAALRAVQNASPFPPLPRAYRHDNLGVNLIVR"
     gene            3591..>4546
                     /locus_tag="VEL74_18125"
     CDS             3591..>4546
                     /locus_tag="VEL74_18125"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64502.1"
                     /translation="MRRIQAIHLAVATLLLALGLSLPRIFAQQAPPQTEPVQDGQVTL
                     ELRRGQRPLIRLAFPAFRGGQGLSGEAAAAARELEATVRQDLANSGFFEIQGPEQLRS
                     LALTGDVTRDAAAYRAAGNEVLLLGDVRGEPDRIVFEGRLFDLGSGQAILAKRYRGEL
                     KVTRRIGHTFADEVIRYLTGSSGISLSTIAFTSDRTGHKEIFLMDYDGANQRRITGHR
                     STSMSPAWSRGGDGLAYTSFFRGGPGIYFAELASGRKRPIITSGSLNITPTFSPDGSR
                     VAFARSLDGNIEIYTSDRSGGGLRRLTHSNAIDTNPAWSPKGG"
BASE COUNT          708 a         1684 c         1495 g          659 t
ORIGIN      
        1 cgccaacgag ggggcccgcg ccggcgccta ccggtacaag acccgctcgt tcggcctcac
       61 catcctcctc cccatggagg agcagcccaa cccgttcctc gacgaggtgg cccagcactc
      121 caccttcttc tcgcggctcc accagttcat ccgcctgtcg cacgcctata tcgtggtcga
      181 cggcggcatc ggcaccaccc tggaggccat gatggtctgg cagctcctcc aggtgcgcat
      241 gctccacgac cgccaggtcg tcttcgtcgg cccgatgtgg aaggggctcc gcgagtggat
      301 cgagcacgac atcatcggcc gcgggctcgc ctcccctccg gacatcaaca tccccgtctg
      361 ggtcgaccgg ctggaggacg ccttgaaggt ggtcggcgag gcgcgtgacg agttcctccg
      421 ccgccacaag gtcgtgctcc cccctgtcgt cgacgaggag acgggcgacc tgcagaagtc
      481 cccggcccct tccccggccg cggagagata accggcccta ctcctcgtcc gtaagctgag
      541 ctgcccgcca cagctcgcgg tacttctctt gcacataggg atcgagcgac tccgccagat
      601 cctcctggag ggcgttcacc cggatcaact cgatgacgtc ggccaggtcg cggagcctgt
      661 ggggtgccgt cattccggag gccagcttga gctcgaccag agttgggagc ggcagcaacg
      721 cgaaccgctc gccgcgcacg gccgcgcggg ccggatcggg aaaggcgaca ggcttcgcct
      781 ttccgtcccc cggatagtct cccgtcagga ccacgtcgat cgtgacgttc tgggctgtat
      841 cccggacgcc cttgctgcca gggaacttct cgaggtaacc ccgtccgaga caggcctgct
      901 tgaaggactc caggccttcg cggcgcaaca ggacgtcgac gtcgctggtc gtccgctgat
      961 agccatacgc gttgagcgac aaggcgccga cgatggcgta ggggatctcc agcttcgtca
     1021 ggcgatcgac caggcgctcg agggccctat gcacgttcga tgtgttcatg aagaaccttg
     1081 ccgcctcacg ggctccggcg aggaaccttt cctcctcctg ggaacgaagc cggccggctt
     1141 gaactgacgt gtgcttcatc gcaaacctcg ccccactatc ttagctcaca gcccgggctc
     1201 cttcccccga ggtaagattc ccccaaccct cgccgggaga cccggcacgc gctttgctgc
     1261 gcggggggta atggccgccc gtttccggga ttccacgaca acccggcaca tccaggcgcc
     1321 tctgttgccg cgccgttctg gcgcacaggg acgttctggc aggcaggagg ggggcacatt
     1381 tggacaccgc cgagtcgata tcgaaccaag gggagcgtga atgagtggat gttttctgat
     1441 gctgttgcag gtgggtccgg tcccggcggc cggagaggag ggcccgggca tcctcgagtt
     1501 cttctggggg gcgggcccga tggccaagat cgtcctcctg accctggtcc tcttctccct
     1561 cttctcctgg gccgtgatga tcggcaagtc ggtgcagctc cgccgcgccg acgcccagag
     1621 cgagaagttc atggaggtct tccaccgcag ccagcgcttc agtgaggtca acgcccaggc
     1681 cggcaagctc cgcacctccc ctctggtcgg catcttccag gcgggctatg ccgagatcga
     1741 cggccagatc aaggccgccg ccgaggccga ccgccctgcc gaccgcaagg ccggcgcccc
     1801 cggcggctac cggatcacgt cgctgccgtc gctcgagcgc accctggccc gcgccgccgg
     1861 cgtcgagctc cgcggcctgc agcgctgggt gccgttcctc gccacgaccg cgtcggcgac
     1921 accgttcatc ggcctcttcg gcacggtgtg gggcatcatg atggcgttca accagatcgg
     1981 cctctccggc tcgacctcca tcgtcgcggt cgcccccggc atcgccgagg cgctgatcaa
     2041 caccgccgcc ggcctcggcg ccgccatccc cgcgctcatc ggctacaact acttcgcgca
     2101 gcgcatgcgg caggtcaagg cgcggatgga cgacttcatc ctcgagttcc tgaacctggc
     2161 cgagaggaac ttcacctagg ggtccgccat ggccttcaac gtaggcaacg gcaacggcga
     2221 cgaagagtcg gtcctggcgg acatcaacgt cacgccgctg gtggacgtga tgctggtgct
     2281 gctcatcatc ttcatgatcg ccgcccccat gctccaccag gggatcgagg tggcgctccc
     2341 gcgcgccgag gcgcagaacc tgcccatgcg catggaggac cccctcgtcg tctccgtcaa
     2401 ccgggacggc cagatcttcc tgcgcgagga gccggtcgag aaggacagcc tggtggaggt
     2461 gctccaggcc gagatctcct cccgcggcgg cgaggacgac acggtcttcc tcaaggggga
     2521 ccgggagctg ccgtacggcg acatgatcgg gatcctcgac ctcctccacc agggggggat
     2581 cacccgcatc ggcatggtca ccgagcggcc gcagcgggag ggcagacggt aggacatgag
     2641 ccggggtgtc aatcaggtgc tcgcccagcg ggcgaagggt gaagggcgcc gccccgcggc
     2701 gatgagcctg ggcgcggcgc tgctgctgca cgccctgctg gtcggcgccg cgctggggct
     2761 gccgcgcctg cagccgcctc ccccgccgct ggagttcgtg ccggtcaccg tcctcccggc
     2821 ccaggcgctg ggcgtgcgcc gccccgcgcc ggcgcgatcc aagcccgccc ccccgaagcc
     2881 cgcgcccgtc caggaggcgg agcccgccgc tcccgagccg gagccggagc ccgaggcgcc
     2941 cgcgccggag cctgaaccgg agcccgagcc gccggcaccg aagccgcgcg aggaggcgcc
     3001 ggtcctcccc cgggagacgc cgaagaggcc ggagaagaag cccgagccca agccggagag
     3061 gacaccggac aggacgccgg ccccgaagcc ggccgagacc ggccgcaagc cggcggacag
     3121 caagaagccc agctccgaga ccggacggga caccaaggtc cccacgccga aaccggccga
     3181 gcccaggccc tcccagggga cgcagccggg cggcaccggc gaccaggtgg gacgccaggg
     3241 cgccgccacc ggcagcccga ccgggaccac ggccttcggc tccgagatcg ccgggctcga
     3301 caacccggat ttcacctatg gctactacat cgaccggctg ctgtcgctga tcgacgccaa
     3361 ctgggtgcgc ccgaacatgg ggagcggcgt gcgggcgatc atcgcgttcc gcatccagaa
     3421 ggacggcagc atcaccgagc tgcgcgtggc ggagtcttcc ggctacaatg ccttcgacct
     3481 ggcggcgctg cgcgcggtcc agaacgcctc gcccttcccg cccctgccgc gggcctaccg
     3541 tcatgacaac ctgggcgtca acctgatcgt gcgctagcca acgaggatcc atgagacgaa
     3601 ttcaagccat ccaccttgcc gtcgccaccc tcctgctggc tctggggctc tcgctccccc
     3661 gcatcttcgc ccagcaggcc ccgccgcaga ccgagccggt ccaggacggc caggtcacgc
     3721 tcgagctgcg gcgcggccag cgcccgctga tccgcctggc cttcccggcg ttccgcggcg
     3781 gccagggcct gtcgggcgaa gcggccgctg cggcgcggga gctggaggcc acggtgcgcc
     3841 aggacctggc gaactcgggc ttcttcgaga tccaggggcc ggagcagctc cggtcgctgg
     3901 cgctgaccgg cgacgtcacc cgcgacgccg cggcctaccg cgccgccggc aacgaggtgc
     3961 tgctgctcgg cgacgtccgc ggcgagcccg accgcatcgt cttcgagggc cggctgttcg
     4021 acctggggag cggccaggcg atcctcgcca agcgctaccg cggcgagctc aaggtgaccc
     4081 ggcgcatcgg ccacacgttc gccgacgagg tcatccgcta cctgaccggc tcgagcggca
     4141 tctccctgtc gacgatcgcc ttcacgtcgg accgcacggg ccacaaggag atcttcctga
     4201 tggactacga cggtgcgaac cagcggcgga tcaccggcca ccggtcgacc tccatgtcac
     4261 ccgcctggag ccgcggcggc gacggcctgg cctacacctc gttcttccgg ggcgggccgg
     4321 ggatctactt cgcggagctg gcgagcggcc ggaagcggcc gatcatcacc tcgggatcgc
     4381 tcaacatcac cccgaccttc tcgccggacg gcagccgcgt cgccttcgcc cgctcgctgg
     4441 acggcaacat cgagatctac acctcggacc gcagcggcgg cggcctgcgc cggctgaccc
     4501 actcgaacgc catcgacacc aacccggcgt ggagcccgaa gggggg
//
LOCUS       DASPJI010000642        11676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_98201, whole genome shotgun sequence.
ACCESSION   DASPJI010000642 DASPJI010000000
VERSION     DASPJI010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11676)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11676
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98201"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..2286
                     /gene="purL"
                     /locus_tag="VEL74_18130"
     CDS             61..2286
                     /gene="purL"
                     /locus_tag="VEL74_18130"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HYG64503.1"
                     /translation="MSPVDTPRQTAEPPITPEVVRSHGMTDEEYERLRGILGRDPNWT
                     ELGITSALWSEHCSYKSSKVYLKEFPTSGPHVLQGPGENAGVVDIGHGWVAVFKMESH
                     NHPSFIEPYQGAATGVGGILRDIFTMGARPIACMDSLRFGEIDAPRMRFLIDGVVRGI
                     GGYGNCMGIPTVGGETQFHRCYNGNILVNAFALGVARRDRIFHARASGAGNPVLYVGS
                     RTGRDGIHGATMASESFDSESEQKRPTVQVGDPFTEKILLEACLEAMRTGAIVAIQDM
                     GAAGLTSSTFEMAGRGGTGLELDLDRVPLREPNLTPYEMMLSESQERMVIVAKRGREE
                     ELVKVYRRWGLEIEPIGEVTDTGRAVLVWRGEVAADIPIRPLTEEAPVYRRPAEPPAD
                     LAERQRAPEVPQPDDLAGTLERLLATPDFGSKEWIWRQYDHTVRTNTVQGPGGDAAVL
                     LLKGTPAGLAMTSDVNPIYCWLDPRRGGAQAVAEAVRNLACTGAEPVGLTDCLNFGNP
                     ERPEITWQFREAIRGMSEACRALAVPVISGNVSFYNETEGKSIYPTPTIGMVGVIANL
                     ASVPDPCFKRAGDRVVLLGQDRGEHGGSAYLRLLHGIEQGLPPEVDLAAELALAKLLR
                     SLVARGLVRTAHDLSEGGLAIALAEACFGHGLGATLEVPLEGAGLFSETQARAMVACP
                     PASLDRLLAAAEEAGVPAQEIGEVGGDELVVRAGGGLLRASVPHLHEVWSTALPKALG
                     L"
     gene            2581..3975
                     /gene="purF"
                     /locus_tag="VEL74_18135"
     CDS             2581..3975
                     /gene="purF"
                     /locus_tag="VEL74_18135"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HYG64504.1"
                     /translation="MCGIFGIDGCDDAANLTYLGLYALQHRGQESSGIVSWDGAQLHR
                     ERGMGHVSDVFPEEVLRRMPGSRAVGHTRYSTAGSSVLSNAQPIVVKTSMGPLGIVHN
                     GNLTNPVGIRNRLEQEGSIFQTTSDTEVILHLMARNPRRDVVESFMLALEHVEGAYSL
                     LLVTDNGLIAARDPNGFRPLMLGDLNGCPCFASESGPFDLLGARTVRELEPGEVVVAR
                     NGQVESYRLNRLVQPARCIFEHVYFARPDSKVFGDAVSQTRLRMGARLAREAPAVADL
                     VAPVPDSGLYAAMGYSRESGLPLEFGLIRNHYVGRTFIEPKQSIRNFGVKIKLNPVRE
                     LIEGKRVVLIDDSIVRGTTSRKIVGMVKDAGAAEVHLRISAPPTQWPCHYGIDTPTRD
                     ELIASHHTVEEIRQFIGADSLAYLSLDGMLASVSGPRDSYCTACWTGDYPVPISPEDR
                     RQAELFPIRAGGVE"
     gene            3990..4196
                     /locus_tag="VEL74_18140"
     CDS             3990..4196
                     /locus_tag="VEL74_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HYG64505.1"
                     /translation="MIFHVTLNQAEDGWVVAECPALPGCVSQGQDEKEALDNIKEAIT
                     AWLWAEDQKALSQIDEKPVVLVAV"
     gene            4165..4422
                     /locus_tag="VEL74_18145"
     CDS             4165..4422
                     /locus_tag="VEL74_18145"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HYG64506.1"
                     /translation="MKNQSSSSPSESVVRLANISGRDAVRAFERAGWRRLGQVGSHVV
                     LVKDGARANLSIPQHKELSIGTLRALIRHSGLTVEEFMDLL"
     gene            4422..4880
                     /locus_tag="VEL74_18150"
     CDS             4422..4880
                     /locus_tag="VEL74_18150"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HYG64507.1"
                     /translation="MRLKIETLLDRPRDVVWRAFEDPSNLRRWQPSLVRIEPRKGSPG
                     QVGSVSKLFFSGDGPDFSLTETVTGRRAPEELLASYDSSHTHNTVRHRFFDLGDGRTR
                     WVVEAEFQFKGFLRLASMALQGTIEKRLRGDVARFKARLEAGEMEPSARE"
     gene            5310..6263
                     /gene="hemF"
                     /locus_tag="VEL74_18155"
     CDS             5310..6263
                     /gene="hemF"
                     /locus_tag="VEL74_18155"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HYG64508.1"
                     /translation="MLDIHQASAVRNYLLGLQDRICSAVEGGEGTGRFEEEVWERPEG
                     GGGRSRVLSEGTVFEQAGVGFSHVFGPGLPPSATAQRPELAGRSYDAMGVSLVFHPRN
                     PYIPTCHMNVRLFVARKEEAEPVWWFGGGFDLTPYYGFEEDAVHWHRTARAACEPFGA
                     DVYPRFKEHCDRYFFLRHRNEQRGVGGIFFDDLNEWGFERCFELLRSVGAHFLPAYLP
                     ILERRRDLPWGERERDFQLYRRGRYVEFNLVWDRGTLFGLQSGGRTESILMSLPPLVR
                     WRYNWRPEPGSPEARLYEEFLRPRDWADGPGGPGGAAVTPS"
     gene            complement(6250..7677)
                     /locus_tag="VEL74_18160"
     CDS             complement(6250..7677)
                     /locus_tag="VEL74_18160"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYG64509.1"
                     /translation="MPQIIRVGVLTPHKLGVDPRQALDFANVVIPLHIYESPYRPSVD
                     GRGVEPVLLEAPLQVDPAGPRNMAFWAAVRRDRKFSDGTPVTAEAIASSLTATSSFTQ
                     EATVEAKGDRVYFRLRHPNARFDLTLANLNLPVVKKGAGGFVATGPFLYAPDSRSDLV
                     RLVKNPHYNGSVYPDEIHVKVYLPAADGQPTALIDAVAKREVDFTDYLSREQVDKVSG
                     VQKILVPGFCTSALFFNTERIPDARVRRALTFNVDRLALASALYSNPVAYAASSLLPP
                     VLGRVRDRFVFSPERAAPLLREAGFSAPARPWGMLMPSVPRPYLPQPQVVAEKLVERF
                     AALGIQLRLEPAKDMAEFSRRAESGNYDLALWGWIPDTPDQVDYLQALLASSWIPRPG
                     HSAAYCGNLARFKSQAMDAALTSYRSERREETLTEVHRLLQDEAPLLPLLYGTRIAVM
                     SWRFTSRPASFEWRPFLAETPLKKE"
     gene            7782..8480
                     /locus_tag="VEL74_18165"
     CDS             7782..8480
                     /locus_tag="VEL74_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015185741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYG64510.1"
                     /translation="MANESSSYRGVILDIDGTLVDSNEAHVQAWTRAFSEHGFNVPAE
                     EVRRLVGMGGDNLVPAAVGLENEDPRSKSLRERHGELFKEKLPGLRPFPRVRELLQRM
                     KEDGMELVVATSSNPDQMKALLEIADVGDLLEDAASKGDAKSSKPDPDIVQAALERIG
                     LAAGEVVMLGDTPYDVEAAGRIGIRTLALRCGGFTDEDLAGALAIYDNAADLLARYES
                     SPLATASAVMAGRR"
     gene            8512..8886
                     /locus_tag="VEL74_18170"
     CDS             8512..8886
                     /locus_tag="VEL74_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017626506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYG64511.1"
                     /translation="MKLFQGINVVSLSVPDLDAAREFYGQVLGLGAPLYDMPEIGWVE
                     FSGGAAAGNIAVTTNEPAQPPSTRTTVVFNTRDCYESCAELRRRGVQCEEPVAVPGVI
                     TYCTFYDPFGNRLQMCSDPPED"
     gene            complement(8903..9454)
                     /gene="lspA"
                     /locus_tag="VEL74_18175"
     CDS             complement(8903..9454)
                     /gene="lspA"
                     /locus_tag="VEL74_18175"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HYG64512.1"
                     /translation="MSAVPDRPETSLRPTAISRFTRLAPVFFLLLLCVGCDQAVKSAA
                     RRTLAGSDPVSLMAGIVRLEYAENPGAFLSLGASLPPGARFAGFAVLAAIVAGAVLVL
                     VLRGREWHASRVLAFSLILGGGIGNLLDRFFNGGAVVDFVSIGFGGLRTGIFNVADLA
                     ITCGALWLILHTAPWRTPAAPEA"
     gene            complement(9509..10048)
                     /locus_tag="VEL74_18180"
     CDS             complement(9509..10048)
                     /locus_tag="VEL74_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG64513.1"
                     /translation="MDVVTLETERLRLRMFRPEDFEAYAEICSDPEVMRYLTGQPLAR
                     HESWRHLAMITGHWWLRGYGHWALELLADGSLIGRAGFFNPEGWPGFELGWTLGRPHW
                     GKGYATEAARAALEHAFTAMGQDHVISVIHPENRASIRVAERVGETFERRTEIPGVPM
                     PVLVYGISRERWQASRRDA"
     gene            complement(10067..10441)
                     /locus_tag="VEL74_18185"
     CDS             complement(10067..10441)
                     /locus_tag="VEL74_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64514.1"
                     /translation="MVIRRFGVLSVAKIAGAIYALIGLIAGGILALVALLGGFAGAAA
                     EDPSSALASTFVGVGSVVIFPIVYGCLGFIGGALSSALYNLLAGFMGGIQLEIQGGIP
                     ATAAPPAAYHPPAAPRDPGFPG"
     gene            complement(10516..10704)
                     /locus_tag="VEL74_18190"
     CDS             complement(10516..10704)
                     /locus_tag="VEL74_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64515.1"
                     /translation="MNDLYRFEIRELKLRRRELERFFGPQPLADYSDQVRSLKGRYAL
                     LALPLELWTEPAEPASRR"
     gene            complement(10791..>11676)
                     /locus_tag="VEL74_18195"
     CDS             complement(10791..>11676)
                     /locus_tag="VEL74_18195"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYG64516.1"
                     /translation="EELSAALASHHGLGTGQTLLGPGSNEILRLVTTAFAGSSKKIVM
                     ADPAFEVVALYARALGIEVVKVPLTADFRHDVPKMLAAASPGTGLIYVCNPNNPTGSL
                     TPKGELRALLAESRGKAMVLVDEAYHHYAESPDYESVIPLVAEHPHLIVARTFSKVYA
                     LAGLRCGYAVARPDVIEKLWAHRAWDVSNAAALVAARASLGDPAYVERSRRLNRETRD
                     LVTAEMERLGYRTIPSQTNFVMIDLRRESRPVIDALRERGVRVGRVFPALPNHLRVSL
                     GTREQMQTFLAAFRQAVA"
BASE COUNT         1811 a         4011 c         4198 g         1656 t
ORIGIN      
        1 cgccctgttc gccggcgccg tcgaggccgg agcccgggcc gtcggagcgg gggcgcggcg
       61 atgagcccgg tggatactcc ccgccagacg gcggagccgc cgatcacccc ggaggtcgtc
      121 cgctcccacg ggatgacgga cgaggagtac gagcgcctgc gggggatcct cgggcgcgat
      181 cccaactgga ccgagctggg catcacctcg gcgctgtggt ccgagcactg ctcctacaag
      241 tcctccaagg tctatctgaa ggagttcccc acctcggggc cgcacgtgct ccaggggccg
      301 ggcgagaacg ccggcgtggt cgacatcggc cacggctggg tggcggtgtt caagatggag
      361 agccacaacc acccgagctt catcgagccc taccaggggg cggcgaccgg ggtgggaggc
      421 atcctgcgcg acatcttcac catgggcgcg cgccccatcg cctgcatgga ctccctgcgc
      481 ttcggcgaga tcgacgcgcc gcgcatgcgc ttcctcatcg acggcgtggt gcgcggcatc
      541 ggcggctacg gcaactgcat gggcatcccg accgtcgggg gggagaccca gttccaccgc
      601 tgctacaacg gcaacatcct ggtcaacgcc ttcgccctgg gggtcgcccg gcgcgaccgc
      661 atcttccacg cccgggcgag cggcgccggc aacccggtgc tctacgtcgg cagccgcacc
      721 ggccgcgacg gcatccacgg cgccacgatg gcgtcggagt cgttcgactc cgagagcgag
      781 cagaagcggc cgacggtgca ggtgggcgat ccgttcaccg agaagatcct gctcgaggcc
      841 tgcctggagg ccatgcgcac cggcgccatc gtcgccatcc aggacatggg ggcggcgggg
      901 ctcaccagct cgaccttcga gatggcgggg cggggcggca cgggcctcga gctcgacctc
      961 gaccgggtgc cgctgcgcga gcccaacctc accccctacg agatgatgct ctccgagtcg
     1021 caggagcgca tggtcatcgt ggcgaagcgg ggccgcgagg aggagctggt caaggtctac
     1081 cggcgctggg gcctcgagat cgagccgatc ggcgaggtca cggacaccgg ccgggccgtg
     1141 ctcgtctggc gcggcgaggt ggctgcggac atcccgatcc gcccgctcac cgaggaggcg
     1201 ccggtctatc gccggccggc cgagccgccg gcggacctcg ccgagcgcca gcgcgcgccc
     1261 gaggtgccgc agccggacga cctcgccggc accctggagc ggctgctggc gacccccgac
     1321 ttcggcagca aggagtggat ctggcgccag tacgaccaca cggtgcgcac caacaccgtg
     1381 cagggtcccg ggggcgacgc ggcggtgctg ctgctcaagg ggacgccggc cggcctcgcc
     1441 atgacgtcgg acgtcaaccc catctactgc tggctcgacc cgcggcgcgg cggcgcccag
     1501 gcggtggccg aggcggtgcg caacctcgcc tgcacgggcg ccgagccggt gggcctcacc
     1561 gactgcctga acttcggcaa ccccgagcgg ccggagatca cctggcagtt ccgcgaggcg
     1621 atccgcggca tgtccgaggc ctgccgggca ctggctgtgc cggtgatctc gggcaacgtc
     1681 tccttctaca acgagaccga gggcaagtcg atctacccga cgccgaccat cggcatggtg
     1741 ggagtgatcg ccaacctggc gagcgtaccg gacccgtgct tcaagcgggc cggggaccgg
     1801 gtggtgctcc tgggccagga ccgcggggag cacggcggct cggcctatct ccgcctcctc
     1861 cacggcatcg agcaggggct gccgcccgag gtggacctcg ccgccgagct ggccctggcc
     1921 aagctgctcc gctcgctcgt cgcccgcggc ctcgtccgca ccgcccacga cctgtcggag
     1981 ggcggcctcg ccatcgccct ggccgaggcc tgcttcggcc acggcctcgg cgccaccctc
     2041 gaggtcccgc tggagggggc cggcctcttc tccgagaccc aggcgcgggc gatggtcgcc
     2101 tgtccccccg cctcgctcga ccggttgctc gccgcggccg aggaggccgg agtgccggcc
     2161 caggagatcg gtgaagtcgg aggcgacgag ctggtcgtcc gcgccggagg cggccttctc
     2221 cgcgcctccg tcccccatct ccatgaggtc tggtccaccg ccctccccaa ggcgctcggc
     2281 ctgtgaccca ccctcagcca cagaatcggg cggggggccg ggaggcttcc cgctccgcgc
     2341 aggcgactgg cagcgagggc ggggggccgg gcggagcgca cacgaaggag ccgagcagga
     2401 gcgggaagcc tcccggcagg ggggcaaccg ccgcctgggg gctcgccctg ggtatcctgg
     2461 cctaaccggg gtatgagaat attgggcaaa gatcgcccgt tgaaagtgaa gccggacacg
     2521 aaccgggacc ggactctctt ctgatctcga actctggaat ggacgcagga cggatcctcc
     2581 atgtgcggaa tcttcggcat cgacggctgt gacgacgcgg ccaatctgac ctacctgggc
     2641 ctctacgccc tgcagcaccg cgggcaggag agctcgggca tcgtctcgtg ggacggcgcg
     2701 cagctccacc gcgagcgggg gatggggcac gtctcggacg tcttccccga ggaggtgctc
     2761 cggcgcatgc ccggcagccg cgcggtgggg cacacccgct actcgacggc gggctccagc
     2821 gtcctctcga acgcccagcc catcgtggtg aagacctcca tggggccgct gggcatcgtg
     2881 cacaacggca acctgaccaa tccggtcggc atccgcaacc gtctggagca agagggctcc
     2941 atcttccaga ccaccagcga caccgaggtg atcctccacc tcatggcgcg caacccgcgg
     3001 cgcgacgtgg tggagtcgtt catgctcgcg ctggagcacg tcgagggggc ctattcgctc
     3061 ctcctggtga ccgacaacgg cctgatcgcg gcgcgggacc cgaacggatt ccggccgctg
     3121 atgctgggag acctgaacgg ctgcccctgc ttcgcctcgg agagcggccc gttcgacctc
     3181 ctgggggcgc ggacggtgcg cgagctggag ccgggggagg tggtggtggc gcgcaacggc
     3241 caggtcgaga gctaccgtct gaaccggctg gtgcagccgg cgcgctgcat cttcgagcac
     3301 gtctacttcg cccggccgga cagcaaggtc ttcggcgacg cggtgtcaca gacgcggctc
     3361 cgcatgggag cgcgcctggc gcgggaggcg ccggcggtgg cggacctcgt cgcgccggtg
     3421 ccggacagcg gcctctacgc cgccatgggc tacagccgcg agtcgggcct gccgctggag
     3481 ttcgggctga tccgcaacca ctacgtgggc cggacgttca tcgagcccaa gcagtcgatc
     3541 cggaacttcg gggtgaagat caagctgaac ccggtgcgcg agctgatcga gggcaagcgg
     3601 gtggtgctga tcgacgactc gatcgtccgc ggcaccacct cgcgcaagat cgtaggcatg
     3661 gtgaaggacg ccggcgccgc cgaggtccac ctgcggatct ccgcgccgcc gacccagtgg
     3721 ccctgccact acggcatcga caccccgacg cgggacgagc tgatcgcttc gcaccacacc
     3781 gtcgaggaga tccgccagtt catcggcgcg gacagcctcg cctacctctc gctcgacggc
     3841 atgcttgcct cggtgagcgg cccccgcgac agctactgca ccgcctgctg gaccggcgac
     3901 taccccgtcc ccatctcccc cgaagaccgg cgccaggccg agctcttccc catccgggcg
     3961 gggggagtgg agtagggaag gagggtgaaa tgatcttcca tgtgactctg aatcaggcgg
     4021 aggacggttg ggtggtcgcg gagtgccccg cccttcccgg gtgcgtgtcg caggggcagg
     4081 acgaaaagga agctctcgac aacatcaaag aggcgatcac cgcctggctc tgggcagagg
     4141 atcagaaggc cctctcccag atcgatgaaa aaccagtcgt cctcgtcgcc gtctgagtct
     4201 gtggtccgtc tagccaatat ttcgggcaga gacgccgtcc gggccttcga aagggctggc
     4261 tggcgccgtt tgggtcaggt cggcagccac gtcgtcctgg tcaaagacgg ggctcgggcc
     4321 aatctttcca tccctcagca caaagagctg tcgatcggca cgctccgtgc cctgatccgt
     4381 cattcgggcc tgacggtaga agaattcatg gatcttctct gatgcggctg aagatcgaaa
     4441 ccctcctcga ccgtccccgg gacgtggtct ggcgcgcctt cgaagatccc tccaacctcc
     4501 gcaggtggca gccgtcgctg gtgcggatcg agccgcggaa ggggagcccc ggccaagtcg
     4561 gctcggtgtc gaagctgttc ttctccgggg acgggcccga cttcagcctc accgaaacgg
     4621 tgaccggccg ccgggcgccc gaggagctcc tcgcctccta cgacagcagc cacacccaca
     4681 acacggtccg tcaccgcttc ttcgacctgg gcgacgggcg cacccgctgg gtggtggagg
     4741 cggagttcca gttcaagggc ttcctgcgcc tggcttccat ggcgctccag ggcacgatcg
     4801 agaagcggct gcggggagac gtcgcccgct tcaaggcgcg gctggaggca ggagagatgg
     4861 agccgagcgc ccgagagtag cgggtgcttc cggcgcctca tcgaagcttc cggcgtgctg
     4921 ccggagtttc cggcgccctg ccgaagcttc cggctcgcca tcagagtctc cgacgggtca
     4981 tccgaagctg ccggcgtgct gccgaagctg ccggcgcgct gccggagctt tcgaggggct
     5041 gccggagttt ccaaccggcc atcggaagct ccgacgtgct gccgaaggct ccgcggggcc
     5101 ggcggactct ccgacgcgcc agcggaagct tccgatgggc tatcgaacgt cccgacgtgc
     5161 tgccggaatc ttcggcgcgc cgccggagac ttcgccaagc ggccggaagc tccgccgaga
     5221 gaaaagcgcc tcccccgcgg cggccctgtc tcgccggcgg tgtcagacct ccagaaaggg
     5281 gcgggttctt tccgctaaac tccccctccg tgctcgacat ccaccaggcc tccgccgtcc
     5341 gcaactatct tctcggcctc caggaccgca tctgctccgc cgtcgaaggg ggggagggga
     5401 ccgggcgctt cgaggaggag gtgtgggagc ggccggaggg gggtggcggg cggtcgcggg
     5461 tgctgtccga ggggacggtg ttcgagcagg cgggggtcgg cttctcccat gtgttcgggc
     5521 ccggcctgcc gccgtcggcc acggcgcagc gcccggagct cgccggccgg agctacgacg
     5581 cgatgggggt ctccctcgtc ttccatccgc gcaaccccta tatccccacc tgccacatga
     5641 acgtccgcct gttcgtcgcc cggaaagagg aggccgagcc ggtctggtgg ttcggcggcg
     5701 gcttcgacct cacgccctac tacggcttcg aggaggacgc cgtccactgg caccgcaccg
     5761 cccgggccgc ctgcgagccg ttcggcgccg atgtctaccc gcgtttcaag gagcactgcg
     5821 accgctactt cttcctgcgc caccgcaacg aacagcgggg cgtcggcggg atcttcttcg
     5881 acgacctgaa cgagtggggg ttcgagcgct gcttcgagct cctgcggagc gtgggcgccc
     5941 acttcctgcc ggcctatctg cctatcctgg aacgccggcg ggatctcccc tggggggagc
     6001 gggagcgcga cttccagctc taccggcgcg gccgctacgt ggaattcaac ctggtgtggg
     6061 accgcgggac cctgttcggc ctccaatccg gcgggcggac cgagtcgatc ctcatgtcgc
     6121 tgccgccgct ggtgcgctgg cgctacaact ggcgcccgga gccgggcagt cccgaggcgc
     6181 ggctttacga ggagttcctg cgcccccggg actgggccga cggtcccggg ggtcccgggg
     6241 gagccgcggt cactccttct tgagcggggt ctcggcgagg aacggccgcc actcgaagct
     6301 ggcgggccgg gaggtgaacc gccaggacat cacggcgata cgcgtgccgt agagcagcgg
     6361 cagcagcggc gcctcgtcct ggagcagccg gtggacttcc gtgagcgtct cctcgcgccg
     6421 ctcactgcgg tagctcgtca gcgccgcgtc catcgcctgg ctcttgaagc gggccaggtt
     6481 gccgcagtag gcggcggagt ggccgggccg cgggatccag gacgaggcga gcagcgcctg
     6541 gaggtagtcg acctggtcgg gggtgtcggg gatccacccc cagagggcga ggtcgtagtt
     6601 gccggactcg gcccggcgcg agaactccgc catgtccttg gccggctcga ggcgcaactg
     6661 gatccccagg gcggcgaagc gctccacgag cttctccgcc accacctgcg gctgcggcag
     6721 ataggggcgg ggcacggagg gcatcagcat cccccacggg cgggccggcg cgctgaagcc
     6781 ggcctcgcgc agcagcggcg ccgcccgctc cgggctgaac acgaagcggt cccgcacccg
     6841 ccccagcacc ggcggcagga gcgacgaggc ggcgtaggcc accgggttcg agtacagggc
     6901 cgaggcgagg gccagccggt ccacgttgaa ggtcagcgcc cgccgcaccc gggcatccgg
     6961 gattcgctcc gtgttgaaga acagggccga ggtgcagaag ccggggacca ggatcttctg
     7021 gacgccggag accttgtcca cctgctcgcg cgacaggtag tcggtgaagt ccacttcgcg
     7081 cttcgccacg gcgtcgatga gcgccgtggg ctggccgtcg gccgccggca ggtagacttt
     7141 gacgtggatc tcgtccgggt agaccgagcc attgtagtgg gggttcttca ccaggcgcac
     7201 caggtccgac cgggagtccg gggcgtagag gaaggggccg gtggcgacga agccgcccgc
     7261 gccctttttg accaccggca ggttgaggtt ggcgagcgtc aggtcgaacc gggcgttggg
     7321 gtggcgcagc cggaagtaga cccggtcccc cttggcctcg accgtcgcct cctgggtgaa
     7381 gctggaggtg gcggtgaggg aggaggcgat cgcctcggcg gtgaccggcg tgccgtccga
     7441 gaacttgcgg tcgcgccgga ccgcggccca gaacgccatg ttgcgcggcc cggccgggtc
     7501 gacctggaga ggcgcttcca ggagcaccgg ctcgacgccc cggccgtcca ccgacggccg
     7561 gtaggggctc tcgtagatgt gcaacggaat caccacgttg gcgaagtcca gggcttgccg
     7621 tggatcgacg ccgagcttat ggggggtgag gactccgaca cggatgatct gaggcatcgt
     7681 gctcttcacg ggctaaagat gaatagcgca cacgatacca cagcacgcga cgccagggcc
     7741 gccgcggagc gcctggtata cctcttgcgg tctccccggt catggcaaac gaaagcagct
     7801 cttatcgcgg cgtcatcctg gacatcgacg gcaccctggt ggacagcaac gaggcccacg
     7861 tgcaggcctg gacccgggcc ttctccgagc acggcttcaa cgtccccgcc gaggaggtcc
     7921 ggcgtctggt cggcatgggg ggagacaacc tggtcccggc ggcggtgggg ctggagaacg
     7981 aggatccgcg gagcaagtct ctccgcgagc ggcacggcga gctgttcaag gagaagctcc
     8041 ccggcctgcg ccccttccca cgggtaaggg agctcctgca gcggatgaag gaggacggga
     8101 tggagctggt ggtcgccacc tcttcgaacc ccgaccagat gaaggcgctc ctcgagatcg
     8161 ccgacgtcgg cgacctgctg gaggacgccg cctcgaaggg ggacgccaag agctccaagc
     8221 cggatcccga catcgtccag gccgcgctgg agcgtatcgg cctcgcggcc ggggaggtgg
     8281 tgatgctggg cgataccccg tacgacgtcg aggccgctgg caggatcggc atccgcaccc
     8341 tggccctgcg ctgcggcggc ttcaccgacg aggatctggc cggagccctg gccatctatg
     8401 acaacgcggc ggacctcctg gcgagatacg agtcgtcgcc gctggccacg gctagcgcgg
     8461 tcatggccgg gaggcgctag gcatgaccgt agcgccgggc aggttgatac ggtgaagctg
     8521 ttccaaggga tcaacgtggt ctccctctcc gtgcccgacc tggacgcggc gcgcgagttc
     8581 tacggccagg tgctcggcct cggcgcaccg ctctacgaca tgcccgagat cggctgggtc
     8641 gagttcagtg gtggagccgc ggcggggaac atcgccgtga ccacgaacga gcccgcccag
     8701 ccgcccagca cccgcacgac cgtggtattc aatacccgcg actgttacga aagctgcgct
     8761 gagctccggc gccggggcgt gcaatgtgag gagccggtcg ccgtgccggg agtgatcacg
     8821 tactgcacct tctacgatcc gttcggcaac cggctgcaga tgtgcagcga tccgccggag
     8881 gattgagcaa ggggctcccc cgtcaggctt ccggcgccgc cggggtccgc cagggggcgg
     8941 tgtggaggat cagccagagc gcgccgcagg tgatggcgag gtcggcgacg ttgaagatgc
     9001 cggtccgcag gccgccgaag ccgatgctca cgaagtccac cacggcgccg ccattgaaga
     9061 agcggtcgag caggttgccg atcccgccgc ccaggatcag cgagaaggcg aggacccgcg
     9121 aggcgtgcca ctcgcggccg cgcagcacga ggaccaggac ggcccccgcc acgatggcgg
     9181 cgagcaccgc gaagccggcg aagcgcgctc ccggcggcag gctggcgccg aggctcagga
     9241 aggcgcccgg gttctccgcg tactccaggc ggacaatgcc ggccatgagc gagacggggt
     9301 cggagcccgc gagcgtccgc cgtgccgccg acttgaccgc ctggtcacag ccgacgcaga
     9361 ggaggagcag gaagaaaaca ggggccagcc gggtaaagcg gctgatggcg gtgggacgca
     9421 gagaggtctc ggggcgatcg ggtacggcag acacggcaac ctacacagtc gagagatgtg
     9481 gagggattct agcaggaaag cggccggatc acgcgtcgcg ccgtgacgcc tgccaccttt
     9541 cccgggagat accgtagacc agcacgggca tcggcacgcc ggggatctcg gtccgccgct
     9601 cgaaggtctc gccgacgcgc tcggcgacgc ggatggaggc ccggttctcc gggtggatga
     9661 cgctgatgac gtgatcctgc cccatcgccg tgaaggcgtg ctccagggcg gcgcgcgccg
     9721 cctcggtcgc gtagcccttc ccccagtgcg gccggcccag cgtccagccc agctcgaagc
     9781 cgggccagcc ctccgggttg aaaaaaccgg cccggccgat gagggagccg tcggctagaa
     9841 gctccagcgc ccagtggcca tagccccgca gccaccagtg ccccgtgatc atcgccaggt
     9901 ggcgccagga ctcgtggcgc gccagcggct ggccggtgag gtaccgcatg acctcggggt
     9961 cggagcagat ctccgcgtag gcctcgaagt cctccggccg gaacatgcgc agccgcaggc
    10021 gctcggtctc gagggtgacc acgtccatcg tgccgctccg gaagggtcag ccggggaagc
    10081 ccggatcccg gggagcggcc ggggggtggt aggcggccgg aggggccgcc gtggccggaa
    10141 tcccgccctg gatctccagc tggatcccgc ccatgaaacc ggcgagcagg ttgtagagcg
    10201 ccgacgagag ggcgccgccg atgaagccca ggcagccgta gacgatgggg aagatgacga
    10261 ccgagccgac gccgacgaag gtgctcgcga gggctgagct ggggtcctcc gcggccgcgc
    10321 ccgcgaagcc gcccagcagt gcgaccagcg ccaggatgcc gccggcgatg agtccgatca
    10381 acgcgtagat cgcgcccgcg atcttcgcca ccgacagcac accgaaccgc ctgatgacca
    10441 tgagtcccct cccttttcga gaacctgctg aaactgctgt cccgctatgc tatgccgccc
    10501 cccacgcagg gtcaatcagc gccggctcgc cggctcggcg ggctccgtcc agagctccag
    10561 ggggagcgcc aggagggcgt atctgccctt gagcgacctc acctggtccg agtagtccgc
    10621 cagcggctgc ggcccgaaga accgctcgag ctcgcggcgc cgcagcttca gctcgcggat
    10681 ctcgaaccgg tagaggtcgt tcaacagctc ccgcaggcgg gccggcggcg tcgtgggccg
    10741 gggccggatg ccgaaacgtt ccagctcccg caggacggag tcccggtacc tcaggccacg
    10801 gcctgccgga aggcggcgag gaaggtctgc atctgctcgc gggtgccgag gctcacccgc
    10861 agatggttgg gcagagccgg gaagaccctc cccacgcgta ccccccgctc ccgcagggcg
    10921 tcgatcaccg gccgggactc gcggcgcagg tcgatcatga cgaagttggt ctgcgacggg
    10981 atggtgcggt agcccagccg ctccatctcg gcggtcacca ggtcgcgggt ctcgcggttg
    11041 agccggcgcg accgctccac gtacgcggga tcgccgaggc tcgcccgggc ggccaccagc
    11101 gccgcggcgt tggagacgtc ccacgcccgg tgggcccaga gcttctcgat gacgtcgggc
    11161 cgggccaccg cgtagccgca gcgcaggccg gccagcgcgt agaccttgga gaaggtgcgg
    11221 gccacgatga ggtgcgggtg ctccgcgacg agggggatga cactctcgta gtccgggctc
    11281 tcggcgtagt ggtgataggc ctcatccacc agcaccatgg ccttgccccg gctctcggcg
    11341 agcagcgcgc gcagctcccc cttgggggtg aggctccccg tggggttgtt cggattgcag
    11401 acatagatca aaccggtgcc gggcgaggcg gcggccagca tcttcgggac gtcgtgccgg
    11461 aaatcggcgg tgagcggcac cttgaccacc tcgatgccca gggcccgggc gtagagggcc
    11521 acgacctcga aggcgggatc ggccatgacg atcttcttgc tggagccggc gaacgcggtg
    11581 gtcacgagcc gcaggatctc gttcgagccg ggcccgagga gggtctggcc ggtcccgagg
    11641 ccgtgatggc tcgcgagcgc cgcgctgagc tcctcg
//
LOCUS       DASPJI010000643         3790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_388356, whole genome shotgun sequence.
ACCESSION   DASPJI010000643 DASPJI010000000
VERSION     DASPJI010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388356"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VEL74_18200"
     CDS             <1..682
                     /locus_tag="VEL74_18200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG64517.1"
                     /translation="TVERFEMVRDRPIYAGIMLDTSGSMQEEIDEAIKGALGFFEKVI
                     TPKDRAAVFTFADRPELAVRFTNQPQVLAGGLAGLHSEGETALYDSLIYGLYYFGGLK
                     GKRAIILLSDGADTKSRYTFNEALDYARRSGVAFYTIGLQLPTNATDIRMKLQKLAEE
                     TGGRSYFIDKARQLEEIYGKIETELRSQYLLAYQSSNADRNDKFRNVEVKVSRPGLDA
                     RTIRGYYP"
     gene            697..1296
                     /locus_tag="VEL74_18205"
     CDS             697..1296
                     /locus_tag="VEL74_18205"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HYG64518.1"
                     /translation="MPDLVLASASPRRRELLAGLGLRYVVRPADVDESVRPGEPPADY
                     VLRLAIEKAEALARPGELVLAADTTVVIDDEILGKPGDADEARRMLRQIAGREHTVLS
                     GVALFEPDRAKGRRAAALESSRVRMAALDDEQIGWYVSTGEPMDKAGSYAIQGLGAMF
                     VEAVFGNYTNVVGLPLPLTRRLFAELGYDIRDFSPAPRT"
     gene            complement(1274..1591)
                     /locus_tag="VEL74_18210"
     CDS             complement(1274..1591)
                     /locus_tag="VEL74_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64519.1"
                     /translation="MSSNTPIRAYLIFTGSSPILILSTYPQLSDERLVDKLRYKGIDK
                     FIAYEVALEAVRERYAHSFDNVARDLQAGEDMRVMDFNGHQIMSNFSLQELGDPIKFE
                     GQG"
     gene            1843..2499
                     /gene="can"
                     /locus_tag="VEL74_18215"
     CDS             1843..2499
                     /gene="can"
                     /locus_tag="VEL74_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HYG64520.1"
                     /translation="MRVLSELLANNRAWATEMTRQDPDFFSRLSRQQAPKYLWIGCSD
                     SRVPANQIVGLMPGEMFVHRNVANVVVHADLNCLSAIQFAVDVLRVDHIIVCGHYGCG
                     GVLAALRDERVGLADNWLRHVQDVRWKHQAELDELETEAERHNRLCELNVIEQVVNVS
                     QTTVVREAWGRGQRLAVHGWIYDLRDGLLRDLGMCMAGDTDSGAGLEPAQPGVSSAMR
                     "
     gene            2534..2977
                     /locus_tag="VEL74_18220"
     CDS             2534..2977
                     /locus_tag="VEL74_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64521.1"
                     /translation="MTPSPKALALVLAMGLLALIAGSAAGAAPRCTSADAALHEAGTW
                     AALHRWFARYSECEEGDLPARVSAVVTTSLAKDWRDLERLERRVDRDPDFEEFVLRHI
                     DATADRAHLEAIAENATQRCPARSSDLCIEVLKRALEALNKPARP"
     gene            complement(2984..3430)
                     /locus_tag="VEL74_18225"
     CDS             complement(2984..3430)
                     /locus_tag="VEL74_18225"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HYG64522.1"
                     /translation="MADAREKQRFLQFLRDRGQRVTSERLTLFDEIFSLHGHIDAEQL
                     LASMTNRGLKISRATVYRNLELLVECGLARKQRLGQSRFLYEHVHSGQQHDHLVCTGC
                     GRVVEFVSPGIAALQSEICRAHGFMPKYHSLQISGVCNQCAGQAGG"
     gene            3609..>3790
                     /locus_tag="VEL74_18230"
     CDS             3609..>3790
                     /locus_tag="VEL74_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64523.1"
                     /translation="MARVTKDEENAEQRSDVDGPSRPRASRPEPDDAWEYEEILDDES
                     GEPLLRLPLHPHRHRRL"
BASE COUNT          619 a         1309 c         1288 g          574 t
ORIGIN      
        1 gaccgtcgag cgcttcgaga tggtgcgcga ccggccgatc tacgccggca tcatgctcga
       61 cacctccggc tcgatgcagg aggagatcga cgaggcgatc aagggggcgc tcggcttctt
      121 cgagaaggtg atcaccccca aggaccgggc ggcagtcttc accttcgccg accggccgga
      181 gctggcggtc cgcttcacca accagccgca ggtgctggcg ggcggcctcg ccgggctgca
      241 cagcgagggg gagacggcgc tctacgatag cctgatctac ggcctctact acttcggcgg
      301 cctcaagggg aagcgggcga tcatcctgct gtcggacggc gcggatacca agagccgcta
      361 caccttcaac gaggcgctgg actacgcgcg gcgcagcggc gtcgccttct acaccatcgg
      421 cctgcagctg ccgaccaacg ccacggacat ccgcatgaag ctccagaagc tggccgagga
      481 gacgggcggg cgctcgtact tcatcgacaa ggcgcggcag ctcgaggaga tctacggcaa
      541 gatcgagacg gagctgcggt cgcagtacct gctcgcctac cagtccagca acgccgaccg
      601 gaacgacaag ttccggaacg tcgaggtcaa ggtctcccgg cccgggctcg acgccaggac
      661 gatccgggga tactacccct gagcccccgg gggccgatgc ccgacctggt cctcgcctcc
      721 gcctcgccgc ggcggcgcga gctgctggcc ggcctcggcc tgcgctacgt cgtccgtccc
      781 gccgacgtcg acgagtcggt gcggccgggc gagccccccg ccgactacgt gctgcgcctc
      841 gccatcgaga aggccgaggc gctggcgcgc cccggcgagc tggtgctcgc cgccgacacc
      901 accgtggtca tcgacgatga gatcctcggc aagcccggcg acgcggacga ggcgcggcgc
      961 atgctgcggc agatcgccgg ccgcgagcac acggtgctct ccggcgtcgc cctgttcgag
     1021 ccggatcggg cgaaggggcg ccgcgccgcc gccctggaga gcagccgggt gcggatggcc
     1081 gccttggacg acgagcagat cggctggtac gtgtccaccg gcgagccgat ggacaaggcc
     1141 ggctcctacg ccatccaggg gctcggcgcc atgttcgtgg aggcggtgtt cggcaactac
     1201 accaacgtgg tcgggctgcc gctgccgctg acccggcggc tcttcgccga gttgggctac
     1261 gacatccgcg acttcagccc tgcccctcga acttgatcgg gtcgcccagc tcctggagcg
     1321 agaagttgga catgatctgg tggccgttga agtccatgac ccgcatgtcc tcgccggcct
     1381 ggaggtccct ggccacgttg tcgaacgagt gggcgtagcg ctctcggacc gcctcgaggg
     1441 ccacctcgta ggcgatgaac ttgtcgatcc ccttgtagcg cagcttgtcg accagcctct
     1501 cgtcggagag ctggggatag gtcgacagga tcaggatcgg gctgctgccg gtgaagatca
     1561 gataggcacg tatcggagtg ttcgagctca tcctctccct ccctgggaag tccagccgaa
     1621 acctagcgcc cccgggcgcg ggctgtcaag gaaggccctc ccccccggcc ccttcgcagg
     1681 gaagctggcc ttctcatcca atctctcccg ctcagagcgg agcccctcca ccttggcccg
     1741 ggacagctcc ggaccaggtc gggacggctc tggctggagg cggacgcctc cgagccaagg
     1801 gggctaccca gagatccccg acggctgtgg tatatctcag ggatgcgtgt cctgagcgag
     1861 ctcctcgcca acaaccgtgc ctgggcgacc gagatgacca ggcaggatcc ggacttcttc
     1921 tctcgcctgt cccgccagca ggctcccaag tacctgtgga tcggctgctc ggacagccgg
     1981 gtgccggcca accagatcgt cggcctgatg cccggcgaga tgttcgtcca ccgcaacgtc
     2041 gccaacgtgg tggtgcacgc cgacctgaac tgtctgtcgg ccatccagtt cgcggtggat
     2101 gtcctgcggg tcgaccacat catcgtctgc gggcactacg ggtgcggcgg cgtgctcgcc
     2161 gccctgcgcg acgagagggt cgggctggcc gacaactggc tgcgccacgt ccaggacgtg
     2221 cgctggaagc accaggccga gctcgacgag ctggagaccg aggccgagcg gcacaaccgg
     2281 ctgtgcgagc tgaacgtcat cgagcaggtg gtgaacgtca gccagacgac cgtcgtgcgg
     2341 gaggcctggg ggcgcggtca gcgcctggcc gtgcacggct ggatctatga cctgcgcgac
     2401 gggctgctgc gcgacctcgg catgtgcatg gccggcgaca ccgacagcgg ggccggtctg
     2461 gaaccggccc aacccggagt ctcctcggcg atgcggtaag atctccccta cagaccgatc
     2521 ccgggagatg atcatgactc cgagcccaaa ggccttggct ctcgtcctcg ccatggggtt
     2581 gctggcgctg atcgccggca gcgccgccgg cgccgcccct cgctgcacgt ccgcggacgc
     2641 ggcgctccac gaggcaggga cgtgggccgc cctccaccga tggttcgcgc gctactccga
     2701 atgcgaggag ggggatctgc ccgccagggt ctcggcggtc gtgaccacct ccctcgccaa
     2761 ggactggcgt gacctcgaaa ggctcgagcg ccgcgtggac cgggaccccg acttcgagga
     2821 gttcgtcctc cgccacatcg acgcgaccgc cgaccgcgcc cacctcgaag cgatcgccga
     2881 gaacgccacc cagcgctgcc ccgcccggtc gtccgacctc tgcatcgagg tgctcaagcg
     2941 ggcgctggaa gcgctgaaca agcccgcgcg gccgtagcca ccctcacccg cccgcctgcc
     3001 cggcgcactg gttgcagacg ccggagatct gcaggctgtg gtacttgggc atgaagccgt
     3061 gggcgcggca gatctcggac tggagcgccg cgatgcctgg gctgacgaac tccaccaccc
     3121 ggccgcagcc ggtgcagacc agatggtcgt gctgctggcc gctgtggacg tgctcgtaga
     3181 ggaagcggct ctggcccagg cgctgcttgc gagcgaggcc gcactccacc agcagctcga
     3241 ggttgcggta gacggtggcg cgggagatct tgagcccccg gttggtcatc gacgccagga
     3301 gctgttccgc gtcgatgtgc ccgtgcaggg agaagatctc gtcgaagagc gtcaggcgct
     3361 ccgacgtgac gcgctgcccc cgatcacgga ggaactggag gaagcgctgc ttttccctgg
     3421 cgtctgccat cgtcgatcga cccttacata ttgagaattc atctcaatcc acaaggcaat
     3481 tctggagtcc tcccggcgtg ttgtcaacct gagtagaggg tagccggacg gcgcagggct
     3541 cccggtatgg aacctgcagc accctgttcc atagggagag gacgatcgac gagggagcag
     3601 gcccagatat ggctcgagtc accaaggacg aggagaacgc ggagcagcgg agcgatgtcg
     3661 acgggccttc acggccccgg gcgtcgcggc cggagcccga cgacgcctgg gagtacgagg
     3721 agatcctgga cgatgaatcc ggtgagccgc tcctccgtct cccgctccac ccgcaccgcc
     3781 accgccgcct
//
LOCUS       DASPJI010000644         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_608848, whole genome shotgun sequence.
ACCESSION   DASPJI010000644 DASPJI010000000
VERSION     DASPJI010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608848"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="VEL74_18235"
     CDS             <1..1280
                     /locus_tag="VEL74_18235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG64524.1"
                     /translation="AIELAAKKSVFQGEDVTVVAIRDITRRKRDEAENKMLQRQLLHS
                     QKMEAIGRLSAGVAHDFNNCLLAIFGYSDLLLAQYREDELLGRNLTGIKDAAQKAAGL
                     TKQLLTFARRQPLETRVMDLNQVVTGLEKMLRRLIGEDIELVTELAPGIARVRIDPGQ
                     MEQVLLNLAVNARHAMPDGGRLVIRTRSLAVSAGAPAPHVDVPPGAHVLLSVTDTGVG
                     MEPEVLSRIFEPFFTTKEIGEGTGLGLSTAYGIVRQSGGHIFVDSAPGKGASFTIYLP
                     ATAEEQTAARDSARAWPDRGTETILLVEDEQEVRAMLHQILLNRGYRVLQAASGEEAL
                     ELAGRHAEAIHLLLTDVIMPRMKGPDLATRLLGARPHTRVLFMSGYNEEALSGAQSTI
                     CLQKPFSPQTLTQTVRAVLDAPDTSALPRAAAM"
     gene            complement(1402..>2235)
                     /locus_tag="VEL74_18240"
     CDS             complement(1402..>2235)
                     /locus_tag="VEL74_18240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64525.1"
                     /translation="HGSVSEVALGATGWDGDVDFGQFGSPFDGANRVVDLFGSILGTM
                     VLALLVCLVLLVARRPLERVEAQLAAEPWKAALAGLAAVFLSVPLLVVVSILLVLTIV
                     GCALFLLYPFLFLGIALLALLGYAAAAYRLGRWLEVRFNRRFGGPYVVALIGVLAIQV
                     WSILGRLIGLPGGPLGFFATMLVLFGGAVQVVAWVMGFGAVLLARFGTGPRPWPGAVA
                     PVGPVPPPAPMYPETPAPDPAYPASYPADPVEPYAPSADGPSPLPEAWEGPRGTGGGG
                     T"
BASE COUNT          372 a          827 c          797 g          239 t
ORIGIN      
        1 tggccatcga gctggcggcc aagaagtcgg ttttccaggg cgaggacgtc accgtcgtgg
       61 cgatccggga catcacgcgc cgcaagcggg acgaggccga gaacaagatg ctgcagcgcc
      121 agcttctgca ctcccagaag atggaggcca tcggccggct ctcggcgggc gtcgcccacg
      181 acttcaacaa ctgcctgctc gccatcttcg gctacagcga cctgctgctc gcccagtacc
      241 gcgaggacga gctgctgggc cgcaacctca ccggcatcaa ggacgcggcg cagaaggcgg
      301 cgggccttac caagcagctc ctcaccttcg cccgccggca gccgctggag acgcgggtga
      361 tggacctcaa ccaggtggtc accggcctcg agaagatgct acgccgcctg atcggcgagg
      421 acatcgagct ggtcaccgag ctcgcccccg ggatcgcccg ggtgcggatc gaccccggcc
      481 agatggagca ggtgctgctg aacctggcgg tcaacgcccg ccacgccatg cccgacggcg
      541 ggcgcctggt gatccgcacc cggtcgctgg cggtgtcggc cggcgcgccg gcgccgcacg
      601 tggacgtccc ccccggcgcg cacgtgctgc tcagcgtcac ggacaccggc gtcggcatgg
      661 agccggaggt gctgtcgcgc atcttcgagc cgttcttcac caccaaggag atcggcgagg
      721 ggacggggct ggggctctcc accgcctacg gcatcgtccg gcaaagcggc ggccacatct
      781 tcgtcgacag cgcgccgggc aagggggcca gcttcaccat ctacctgccg gcgaccgccg
      841 aggagcagac ggcggcgcgc gacagcgccc gcgcctggcc cgaccggggc accgagacca
      901 tcctgctggt ggaggacgag caggaggtgc gcgccatgct ccaccagatc ctgctcaacc
      961 gcggctaccg ggtgctgcag gcggcgtcgg gggaggaggc gctggagctc gccggccgcc
     1021 atgccgaggc catccacctg ctgctgaccg acgtgatcat gccgcggatg aagggccccg
     1081 acctcgccac ccggctgctg ggcgcccggc cgcacacccg ggtgctcttc atgtcgggct
     1141 acaacgagga ggcgctctcc ggcgcccagt cgacgatctg cctgcagaaa cccttctccc
     1201 cccagaccct gacccagacc gtccgcgccg tcctcgacgc ccccgacacc tccgcgctcc
     1261 cccgcgcggc ggcgatgtag gcccctcgcg tcgcgggggt cgttctcccg gggcgacgat
     1321 ggccccacga aaaacgaacc gcctccccct ctcccgggga gggaggaggg gaggcgggag
     1381 agggggccgg ggggtgaggg cctacgtgcc gccgccgccg gtgccgcgcg gaccttccca
     1441 ggcctccggc agaggggacg gcccgtccgc cgagggggcg tacggctcga cggggtcggc
     1501 cggataggaa gccgggtagg ccggatcggg ggccggcgtc tccgggtaca taggtgccgg
     1561 cggcgggacc ggtcccaccg gggcaacggc ccccggccag gggcgcgggc cggtgccgaa
     1621 gcgcgccagg aggacggcgc cgaagcccat cacccaggcc accacctgca ccgcgccgcc
     1681 gaacagcacc agcatcgtgg cgaagaagcc gagcggaccg cccggcaggc cgatcaggcg
     1741 ccccaggatc gaccagacct ggatcgccag cacgccgatc agggccacca cgtaggggcc
     1801 gccgaagcgc cggttgaagc ggacctccag ccagcgcccc aaccggtagg ccgccgccgc
     1861 gtagccgagc agggcgagca gcgcgatccc caggaagagg aacggataga ggaggaacag
     1921 cgcgcagccg acgatggtca ggaccagcag gatgctcacc accaccagca gcggcaccga
     1981 gaggaacacc gcggccaggc cggcgagcgc cgccttccac ggctcggcgg cgagctgcgc
     2041 ctcgacccgc tcgagcggcc ggcgggccac cagcaggacc aggcagacca gcagcgcgag
     2101 caccatcgtg ccgaggatcg agccgaagag gtcgacgacg cggttggccc cgtcgaaggg
     2161 ggagccgaac tgcccgaagt ctacgtcgcc atcccagccg gtcgccccca gggccacctc
     2221 cgagaccgag ccgtg
//
LOCUS       DASPJI010000645         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_41672, whole genome shotgun sequence.
ACCESSION   DASPJI010000645 DASPJI010000000
VERSION     DASPJI010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41672"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VEL74_18245"
     CDS             complement(<1..855)
                     /locus_tag="VEL74_18245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG64526.1"
                     /translation="MSLPLPAAAPAVPSPGPRVALIHDWLTGMRGGEKVLAALASLFP
                     GAPLFTLFHFPGRVSQTIESHPIHTSFLQGAPGVERHYRRYLPLFPAAIEEFNLSGFR
                     LVVSSSHCVAKGVIPPPDAFHLCYCHTPMRYAWDQEHVYFPKRTGLGPRLRSLALSGL
                     RAWDAASASRVHLFVANSRFVAQRVRSYYGREAQVVNPPVDVEFFTPGEQEKEDYCLM
                     VTALAPYKRVELAIAACDRVGIELRIVGEGPERERLQTLAGPRTRFLGKVDDQRLREL
                     YRGARLFLQ"
     gene            complement(852..2042)
                     /locus_tag="VEL74_18250"
     CDS             complement(852..2042)
                     /locus_tag="VEL74_18250"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYG64527.1"
                     /translation="MAYTIGIDVRKIQDFGIGTYIRNLVQGLARVDSENRYVLLCRPA
                     DREHLDGLPHNFQPVTESAPVYSVRELVALSWHLWTYGLDLYHSTHYVLPAVVRSPVV
                     VTIHDIIHLLYPDFLPSRFAFLYAQRMIRRSLVRGDRVIAVSQNTRSDLMQYFEMDGQ
                     KIEVVYNGVEEVFRHRLPEDEIERWLRSLGIERPYMLFVGNPKPHKNLDNVVKAYARA
                     RQLGNLDVPLVCVGARSGSEFKIRQRAQYLGIADKVRLLGHVAQEALPAIYQGASLFL
                     YPTLYEGFGLPVVEAMACGVPVITSNTSALKEIAEGYAHLVDPLDTDGMARAMAHLMG
                     DAERRASLARLGARRAEDFRWDETARQTLEIYLSVIEGREPVLDPRPGESPSDRPGEP
                     GGAA"
     gene            complement(2142..>2513)
                     /locus_tag="VEL74_18255"
     CDS             complement(2142..>2513)
                     /locus_tag="VEL74_18255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG64528.1"
                     /translation="ELRPLGIQYLGRVDRGRLVRLFQAARLFVYPSFYEGFGLPPAEA
                     MACGVPAVVSNRSSLPEVVGRAGVLVEPGDPEALAVAIQKILADPGREDDLRARSQEQ
                     AARFRWDRAAQEMEQVFLEAL"
BASE COUNT          393 a          827 c          938 g          355 t
ORIGIN      
        1 ctggaggaac aggcgggcgc cgcggtagag ctcgcgcagg cgctggtcgt ccaccttccc
       61 caggaaacgg gtgcgcggcc cggccagggt ctggaggcgc tcgcgctccg gcccttcgcc
      121 gacgatccgc agctcgatcc ccacccggtc gcaggcggcg atggccagct ccacccgctt
      181 gtagggggcg agcgcggtga ccatcaggca gtagtcctcc ttctcctgct ctcccggagt
      241 gaagaactcg acgtccacgg gcgggttcac cacctgggct tcgcggccgt agtagctgcg
      301 tacgcgctgg gcgacgaacc gcgagttggc cacgaagagg tggacgcgcg acgccgacgc
      361 cgcgtcccac gcccgcaggc cggacagggc gagtgagcgc aggcgggggc ccagcccggt
      421 ccgcttgggg aagtagacgt gctcctggtc ccaggcgtag cgcatgggcg tgtggcagta
      481 gcagagatgg aatgcgtcgg ggggcgggat gacccccttg gcgacacaat ggctggagct
      541 caccaccagc cggaagccgg agaggttgaa ctcctcgatg gccgcgggga agagcggcag
      601 gtagcgccgg tagtggcgct ccacgccggg cgccccctgc aggaagctgg tgtggatcgg
      661 gtggctctcg atcgtctggc tcacccgccc ggggaagtgg aagagggtga agagcggcgc
      721 cccggggaag agggaggcca gggccgccag caccttctcg ccgccgcgca tgccggtgag
      781 ccagtcgtgg atcagcgcca cgcgcggccc ggggctgggg acagcgggcg ccgcggcggg
      841 cagggggagg ctcaagccgc acctcccggc tcgccggggc gatcgctcgg cgactcgccg
      901 ggccgcggat ccagcaccgg ttcccgcccc tcgatgaccg acaggtagat ctccagggtc
      961 tggcgggcgg tctcgtccca gcggaaatcc tcggcgcgcc gggcgcccag gcgagcgagg
     1021 gaggcacggc gttccgcgtc ccccatcagg tgggccatgg cgcgggccat gccgtccgta
     1081 tccagggggt ccaccagatg ggcgtagccc tcggcgatct ccttgagggc cgaggtgttg
     1141 gaggtgatca ccggcacgcc gcaggccatg gcctccacca ccggcagccc gaacccctcg
     1201 tagagggtgg gatagaggaa caggctcgcc ccctggtaga tggcgggcag ggcctcctgg
     1261 gccacgtgcc ccagcaggcg caccttgtcg gcgatcccca ggtattgggc gcgctggcgg
     1321 atcttgaact cggagccgct gcgggcgccg acacagacca ggggcacgtc caggttgccg
     1381 agctggcggg cccgggcata ggccttgacc acgttgtcca ggttcttgtg cggcttgggg
     1441 ttgccgacga agagcatgta ggggcgctcg atccccaggc tgcgcagcca gcgctcgatc
     1501 tcgtcctccg gcagccgatg ccggaagacc tcctcgacgc cgttgtagac cacctcgatc
     1561 ttctggccgt ccatctcgaa gtactgcatc aggtcgctgc gggtgttctg cgacacggcg
     1621 atgacccggt cgccgcgtac gaggctgcgg cggatcatgc gctgggcgta caggaacgcg
     1681 aagcggctgg gcaggaagtc ggggtagagc aggtggatga tgtcgtggat ggtcaccacc
     1741 acgggcgagc ggaccacggc cggcagcacg tagtgggtcg agtggtagag gtcgagaccg
     1801 taggtccaga ggtgccagga gagggcgacc agctcgcgca cggagtagac cggcgcgctc
     1861 tcggtgaccg gctggaagtt gtgcggcagg ccgtcgaggt gctcgcggtc ggcgggccgg
     1921 cagagcagga cgtagcggtt ctcggagtcg acacgggcca gcccctggac caggttgcgg
     1981 atgtaggtcc cgatcccgaa atcctggatc ttccgtacgt cgatgccgat ggtataagcc
     2041 acatccaact cctcgggcag ggaccggacc ttcccgtgcc gccgcccttc aacgggacca
     2101 ggcggcatgg agatccacga gaactgtccg gtgccgctat tctacagcgc ttcgaggaag
     2161 acctgctcca tttcttgtgc tgcccggtcc caacggaagc gggcggcctg ctcctgggac
     2221 cgggcgcgga ggtcgtcctc gcgtcccgga tcggccagga tcttctggat cgccacggcc
     2281 agcgcctcgg ggtctccggg ctccaccagc accccggccc ggcccaccac ctcgggcagg
     2341 ctggagcggt tggagaccac ggcgggcacg ccgcaggcca tggcctcggc cggcggcaag
     2401 ccgaagcctt cgtagaacga cgggtagacg aagagccgcg cggcctggaa gaggcggacg
     2461 agccggccgc ggtccacccg gccgaggtat tggatcccca gggggcgcag ctc
//
LOCUS       DASPJI010000646         6249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_338984, whole genome shotgun sequence.
ACCESSION   DASPJI010000646 DASPJI010000000
VERSION     DASPJI010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6249)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338984"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2897
                     /locus_tag="VEL74_18260"
     CDS             <1..2897
                     /locus_tag="VEL74_18260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64529.1"
                     /translation="TPEQVDATHFQFALQTPQVWLDHQASELAGRLVLSWQVVDGLFP
                     PGLVEAMFEAYCGLVARLGAEEDLWTSPMAVPPPAAHLALAAEANATAAPIAPRPLHA
                     AFTGQARRTPDSPAVVTAGRTVSYGELARCAARLARRLRELGAGPDRLVAVAMEKGWE
                     QAAVVLAVLEAGAAYLPLDPGLPRERRRHLLEAGQVEIVLTQPWLAGGLDGPRALQVI
                     PVTPADLTAPGDGLAAEAPTDSLAYVIFTSGSTGLPKGVAIEHANAWNTVADVNGRFG
                     IGPADRVLALSALSFDLSVWDLFGPMSAGGAVVMPDAAVLREPARWVELMQAEAVTVW
                     NSVPALMEMLVDHLEGRGEPLPATLRLVLLSGDWIPVSLPDRIRRLAPGIQVVSLGGA
                     TEASIWSILHPIQAVDPAWSSIPYGRPMANQTFQVLDENLRPRPVWVPGQLYIGGAGL
                     ARGYWRDEERTRAAFLIHPETGERLYRTGDLGRRLPDGTLEFLGREDLQVKVQGYRIE
                     LGEIEAALLAHPGVRGAVAAAVGPRRGAKRLVAYVVPVADGLDLEELRGFLAAKLPSY
                     MVPAALALLDELPLTANGKVDRQALPDPERTRPHREAVPPRDELEETLARIWSDVLGD
                     TLAGAPVSVHDDFFELGGHSLLAVRLMGRIQAELGRELPLSALFGAPTVEALAGLLRQ
                     EPETPASGRRALVEVRPGGAGLPFFCVHPVGGNVLCYRDLARELGTGQPFFALQTPDP
                     APSTVEEMAAAYLAEVRQVQPRGPYRLGGWSMGGVVAYEMARQLECAGEEVELLAMID
                     PWTPEPGDAGGVDGAVLASWFLGDLGGLTGVKLSIDPEELRPLDPGARAALLLERARE
                     AHVLPPGFGLAELSRHLETFQHNFRALLDYAPGEYPGRPLLLLAADGGAAGTAVEVWS
                     GLAPDAEARLLPGDHYTLVRPPRVTALAEAIATALAAAMAREMAGEMAPR"
     gene            2894..4282
                     /locus_tag="VEL74_18265"
     CDS             2894..4282
                     /locus_tag="VEL74_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64530.1"
                     /translation="MTTTLTAYDDPAVAAALPRVHERFLEFALRRPEYLDPASFVLLG
                     QKDGVLRHPMEGWPLFIGREQAAALGRAAVGIARVAASIPRRVFELDPERMAEFYRLP
                     RELVGLTVSLMAKNDPTDGAIGRGDFILAPSGLKCCEANMAGNLGGSELGDWTERYLR
                     EPLVRRFMEHEGLVGSYTDPPRVLFHHILDEAEERGVPEDGRLNAALLVSPSPPEWRA
                     HFQRVWQSVLADRGGAVEGELAVCGDAELRERGGRLFLGDLRIDVVVDASFGQVARHV
                     FVALQAGRVRAYNGPATPLLSDKLNLALASEMADSGLFTDEERRVIRDHLPWTRRVED
                     GFADFEGERVYLPDLLADRRERLILKHSLSSQGSDVHAGAFTPPARWEELVRAALDDG
                     LWIVQEFMPPTPIPFLLPEGGAADHEVVWGLYIIGERYGGGFLRVQPAGRAGVINSAR
                     GALCTCIIEVDE"
     gene            complement(4632..>6249)
                     /locus_tag="VEL74_18270"
     CDS             complement(4632..>6249)
                     /locus_tag="VEL74_18270"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYG64531.1"
                     /translation="VLLVLLIACVNVGNLLLARAQDQSRESAIRVALGASRGRMVRQW
                     VTSTLLLTLLGGALGLLAAHWGLAAFLAVNPEPVPRAEGIGLDGRVVAFTLGLTLLTA
                     LVLGMVPALRASRPDLQGTFKEGGGKATASAGVHRFRRVLVGLEVALALVLLISAGLT
                     LKSFARLRQVDPGFRATGLLTMELSLPRSRFGEDREASQLYDRLLDRIVALPGVEAAS
                     SVSLLPFAGGDAHRSASVGAEGQPYSPGDALPEPEVRSIDHRYFRTMDIPVLAGRAFT
                     ASDDEKAFPMAVIDDVVARRLWPGTRTLEVVGKRFKLGPPVEQNPAPWVTVVGVAGVA
                     RQFGLGTDESRGVVYFPQLRRAERTQSLIIRAEGDADPLTLAGPVRDAVRTMVPDQPV
                     AQVMTMEQRIAASLASPRFSMLLLAAFAAVAMALAAVGIYGVVAYSVGQRTHEIGVRM
                     ALGAQRRQVLTLVVLQGMSVVLAGLVLGLLASYWATRALAGLLYGVAATDAATFVTVS
                     LLLAAVGLAANLLPARRAANLEPTTALRYE"
BASE COUNT          799 a         2305 c         2312 g          833 t
ORIGIN      
        1 cgacccccga gcaggtggac gcgacccact tccagttcgc cctccagacg ccgcaggtct
       61 ggctcgatca ccaagcctcc gagctggccg gccggctcgt cctctcctgg caggtggtgg
      121 acggcctctt cccgcccggc ctggtggagg cgatgttcga ggcctactgc ggcctggtgg
      181 cccggctggg cgccgaggag gacctctgga cgtcccccat ggccgtgccg ccgccggccg
      241 cccacctggc cctggccgcc gaggccaacg ccacggcggc gccgatcgcc ccacggccgt
      301 tgcacgccgc cttcaccggg caggcgcggc ggacgccgga ctccccggcg gtcgtcacgg
      361 ccgggcggac tgtctcctac ggcgagctgg cccgctgcgc cgcccggctg gcccggcggc
      421 tgcgggagct gggcgccggc ccggaccgtc tggtggcggt ggcgatggag aaggggtggg
      481 agcaggccgc ggtggtcctc gccgtcctcg aagccggcgc cgcctatctc cccctggacc
      541 cgggcctgcc gcgggagcgc cgccggcatc tcctggaggc cgggcaggtc gagatcgtcc
      601 tcacccagcc gtggctcgcg ggcggcctcg acgggccgcg ggcgctccag gtcatcccgg
      661 tgaccccggc cgatctcacc gctccggggg acgggctggc agccgaggcg cctacggaca
      721 gcctggccta cgtgatcttc acctcgggct ccaccggcct gcccaagggg gtggccatcg
      781 agcacgccaa cgcctggaac accgtggccg acgtcaacgg ccgcttcggc atcgggccgg
      841 cggaccgggt gctggccctg tcggcgctct ccttcgacct ctccgtgtgg gacctcttcg
      901 gccccatgtc cgccggcggc gccgtggtga tgcccgacgc cgcggtgctg cgcgagccgg
      961 cgcgctgggt ggagctgatg caggccgagg cggtcacggt gtggaactcg gtgccggcgc
     1021 tcatggagat gctggtggac cacctggagg ggcgcggcga gcccctgccc gccacactga
     1081 ggctggtcct cctctccggt gattggatcc cggtgtcgct gcccgaccgc atccggcggc
     1141 tcgcgcccgg catccaggtg gtgagcctgg ggggcgccac cgaggcgtcc atctggtcga
     1201 tcctccatcc gatccaagcc gtggaccccg cctggagcag catcccttat ggccggccca
     1261 tggcgaacca gacgttccag gtactggacg agaacctccg gccgcggccg gtctgggtgc
     1321 ccggccagct ctacatcggc ggcgcggggc tcgcccgcgg ctactggcgg gacgaggagc
     1381 ggacccgggc cgccttcctc atccaccccg agaccggcga gcgcctctac cgcaccggcg
     1441 acctcggacg ccggctgccc gacggcaccc tcgagttcct ggggcgtgag gacctccagg
     1501 tcaaggtcca gggataccgg atcgagctgg gggagatcga ggccgcgctc ctcgcccatc
     1561 caggggtgcg aggggcggtg gcggcggcgg tggggccgcg ccgcggcgcc aaacgtctgg
     1621 tcgcctatgt cgtaccggtg gcggacggcc tggatctcga ggagcttcgc ggcttcctgg
     1681 cggcgaagct cccctcctac atggtgccgg cggcgctggc gctcctcgac gagctgcccc
     1741 tgaccgccaa cggcaaggtg gaccgccagg cgctgcccga cccggagcgg acccggcctc
     1801 atcgtgaggc ggtgccgccg cgtgacgagc tggaggagac gctcgcccgg atctggagcg
     1861 acgtcctggg cgacaccctg gccggagcgc cggtgtcggt gcacgacgac ttcttcgagc
     1921 tcggcggcca ctccctgctc gccgtccgcc tcatgggccg catccaggcc gagctcggcc
     1981 gcgagctgcc gctgtcggcg ctgttcggcg cccccacggt ggaggccctc gccggcctgc
     2041 tgcggcagga gccggagacc ccggccagtg gcaggcgggc gctggtcgag gtgcggcccg
     2101 gcggcgccgg cctccccttc ttctgtgtcc acccggtggg aggcaacgtc ctctgctacc
     2161 gcgacctcgc ccgggagctg ggcaccggcc agccgttctt cgccctgcag acgccggacc
     2221 cggctccttc cacggtggag gagatggccg ccgcctacct cgccgaggtc cggcaggtcc
     2281 agccgcgcgg cccctaccgc ctgggcggct ggtccatggg cggcgtcgtc gcctacgaga
     2341 tggcgcggca gctcgagtgc gccggcgagg aggtggagct gctggcgatg atcgacccct
     2401 ggacgcccga gcccggcgac gccggcgggg tggacggcgc cgtcctcgcc tcctggttcc
     2461 tgggcgatct gggcgggctc accggggtga agctgtcgat cgatcccgag gagctgcggc
     2521 ctctggaccc cggcgcccgc gccgccctcc tgctggagcg tgcgcgggag gctcatgtgc
     2581 tgcccccggg cttcgggctg gcggagctgt cccgccacct ggagactttc cagcacaatt
     2641 tccgtgccct gctggactac gcccccgggg agtacccggg ccgcccgctc ctcctgctcg
     2701 ccgcggacgg cggcgcggcc ggcacggccg tcgaggtctg gagcgggctc gccccggacg
     2761 ccgaggcccg cctgctgccg ggcgatcact acaccctcgt ccgcccgccc cgggtgacgg
     2821 cgctggccga ggccatcgcg acggcgctgg cggcggccat ggcgagggag atggcggggg
     2881 agatggcgcc gcgatgacca ccaccctgac cgcctacgac gacccggcag tcgcggcggc
     2941 cctgccgcgg gtgcacgagc gctttctgga attcgccctg cgccggcccg agtacctcga
     3001 tcccgccagc ttcgtcctgc tgggccagaa ggacggggtg ctgcgccacc cgatggaggg
     3061 ctggccgctc ttcatcggcc gggagcaggc cgccgccctc ggccgcgccg cggtggggat
     3121 cgcccgcgtc gccgcgtcga tccccagacg cgtcttcgag ctggaccccg agcgcatggc
     3181 cgagttctat cgcctgccgc gcgagctggt gggcctcacc gtctcgctga tggcaaagaa
     3241 cgatccgacc gacggcgcca tcggccgcgg cgacttcatc ctcgccccgt ccggcctcaa
     3301 atgctgcgag gccaacatgg ccggcaatct ggggggaagc gagctggggg actggaccga
     3361 gcgctatctg cgcgagccgc tggtgcgccg cttcatggag cacgaggggt tggtgggctc
     3421 ctacaccgat ccgccgcgcg tcctcttcca tcacatcctc gacgaggccg aggagagggg
     3481 ggtgcccgag gacgggcggt tgaacgcggc cctgctggtc tcgccgtcgc cgccggagtg
     3541 gcgcgcccac ttccagcgcg tctggcagtc ggtgctggcg gaccgcggcg gcgccgtcga
     3601 gggggagctg gcggtctgtg gcgacgccga gctgcgggag cggggcggcc gcctgttcct
     3661 cggcgatctg cggatcgacg tggtggtgga cgccagcttc ggccaggtcg cccggcacgt
     3721 cttcgtcgcc ctccaggccg gcagggtccg ggcctacaac gggccggcca cgccgctgct
     3781 gagcgacaag ctcaacctgg cgctcgcctc cgagatggcg gattcgggcc tgttcaccga
     3841 cgaggagagg cgggtcatcc gcgaccacct cccgtggacc cggcgggtgg aggacggctt
     3901 cgccgacttc gagggggaaa gggtctacct gccggacctg ctggcggacc ggcgcgagcg
     3961 gctgatcctg aagcacagcc tctccagcca ggggagcgac gtgcacgccg gcgccttcac
     4021 gccgcccgcg cgctgggagg agctggtccg cgccgctctg gacgacggcc tctggatcgt
     4081 ccaggagttc atgccgccga cgcccatccc ctttctcctt cccgagggcg gcgccgcgga
     4141 ccacgaggtc gtctggggcc tctacatcat cggcgaacgc tacggcggcg gcttcctgag
     4201 ggtgcagccc gccggccgcg ccggcgtcat caactccgcc cgcggcgccc tctgcacctg
     4261 catcatcgaa gtggacgagt aggcgctgtc ctgacggcgg gccccgcatc ccgcggcgcg
     4321 ttctctcctc cggacgccac ggatgccgtc caggcgccgt ggatctcgtc cggaagctcc
     4381 gggagccttc ccggacgccg cgggcggctc cccgttcaaa cggctggatt ccggatgccg
     4441 cggaagcttc ccgaacggtc cggatcgcgt ccgattcaac gggatggatt ccggatgccg
     4501 cggaagcttt ccgaaggctt cggatcacgc ccacttcaac gggatggatt ccggaagccc
     4561 gggaagcttc ccgcgagctc cggatcgtat ccagctcatg aaagtcggcg cccgtggcgc
     4621 cggcaggggg cctattcgta gcgcagcgcc gtcgtgggct ccaagttggc cgcccggcgg
     4681 gccggcagca ggttggcggc gaggcccacg gcggcgagca gcagcgacac ggtgacgaag
     4741 gtggcggcgt cggtggcggc gacgccgtag agcaggccgg ccagcgcccg ggtcgcccag
     4801 taggaggcga ggaggccgag gacgagcccg gccaggacca cgctcatccc ctggagcacc
     4861 accagcgtca gcacctgccg gcgctgggcg cccagggcca tgcgcacgcc gatctcgtgc
     4921 gtccgctggc cgaccgagta ggcgaccacg ccgtagatcc ccaccgcggc gagcgccatg
     4981 gcgaccgcgg cgaaggcggc gaggagcagc atggagaagc gggggctggc gagcgaggcc
     5041 gcgatccgct gctccatggt catcacctgg gccaccggct ggtccgggac catggtccgt
     5101 accgcatcgc gcaccgggcc ggccagagtc agcggatcgg cgtccccctc ggcacggatg
     5161 atcagcgact gcgtccgctc cgcgcgccgg agctgcggga agtagacgac ccccctagac
     5221 tcgtcggtgc ccaggccgaa ctgccgtgcc acgccggcga cgccgaccac ggtcacccac
     5281 ggggcggggt tctgctccac cggcggcccg agcttgaagc gcttgcccac cacctccagt
     5341 gtccgcgtgc cgggccagag ccgccgcgcc acgacgtcgt cgatgaccgc catgggaaag
     5401 gccttctcgt cgtccgaggc ggtgaaggcg cggccggcga ggacggggat gtccatggtc
     5461 cggaagtagc ggtggtcgat cgagcgcacc tcgggctcgg gcagcgcgtc ccccgggctg
     5521 tacggctgcc cttcggcgcc cacgctggcg gagcggtggg cgtcgccgcc ggcgaacggc
     5581 agcagcgaca ccgacgaggc ggcctcgacg ccgggcagcg cgacgatccg gtcgaggagc
     5641 cggtcgtaga gctggctcgc ctcccggtcc tcgccgaagc gggagcgggg gagggacagc
     5701 tccatggtca gcaggcccgt ggcgcggaag ccggggtcca cctggcgcag gcgggcgaag
     5761 ctcttgagcg tcaggccggc gctgatcagc agcaccaggg ccagcgccac ctccagcccc
     5821 accagcaccc gccggaagcg atggacgccg gcgctcgccg tggccttgcc gcccccctcc
     5881 ttgaacgtcc cctggaggtc cggccgcgac gcgcgcaggg cgggaaccat cccgaggacg
     5941 agcgcggtga gcagggtgag cccgagggtg aaggcgacca cccggccgtc gaggccgatc
     6001 ccctcggcgc ggggcaccgg ctcggggttg acggcgagaa aggcggcgag accccagtgg
     6061 gcggcgagca gccccagcgc gccgcccagc agggtgagga gcagcgtgct ggtgacccac
     6121 tggcgcacca tccggccgcg gctggcgccc agggccacgc ggatcgccga ctcccgggac
     6181 tggtcctgcg cccgcgccag cagcaggttg ccgacgttga cgcaggcgat gagcagcacc
     6241 agcagcacc
//
LOCUS       DASPJI010000647         7067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_41721, whole genome shotgun sequence.
ACCESSION   DASPJI010000647 DASPJI010000000
VERSION     DASPJI010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7067)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41721"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="VEL74_18275"
     CDS             complement(<1..934)
                     /locus_tag="VEL74_18275"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HYG64532.1"
                     /translation="MRRVCLMLALCCLMIPPAGADPRPFLDLDFEAPECTSEWTRLGP
                     TWRAYDSLYDQTQASSGGQSLRIGYVYPFQWVPQLGVGSIAQLFPNEQAAGRKIRFSG
                     SIRTEGVPIWAGLYWIAIDQAGGVTFGNSGAEGAGTTPWTRYEFEIDIPADLQQTYFG
                     VDLVGPGTAWFDDIRIEIDGQPWKDRAPNLFEPTPGHAAWLRGNALPLATAAAGNGFA
                     DLQPLRQAFGDARIVSLGEATHGTREFFLMKHRLLEFLVEEMGFTHFSIEANMPETYK
                     MNEYVLEGQGDPEELLEGMLFWTWNTQEVLDMIHW"
     gene            complement(1125..3650)
                     /locus_tag="VEL74_18280"
     CDS             complement(1125..3650)
                     /locus_tag="VEL74_18280"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HYG64533.1"
                     /translation="MRVLTLGCSLGCSLSLFAAALCAQTLDDARALHAAGRLDEALAA
                     YRVVARSLQGSDPASAASARNNACAILNDRGDYRAALAECQESLRLFQGIDDPLRLSR
                     ALNNTGLSLQYLGRWDEAEQRFREALAINRRTGDVEAQVINLANLGLSETSAGRYGRA
                     LAFHAEAARLAAAHAGEPWAPVQLRVARINQGVVLEKLGAYQEALALYRQVLAEGDAL
                     EPGQRATLEVDTGVVYRNLGDPVRALEEFRQAVAIFEGEGDAGDVAALSNAWLNIGLV
                     HHLNLESPRAAEEAYRKALHYAEASGDRSEEIQDLFYLGRLLLGRGRLGEAERVFRRC
                     LAAAERSGSAEGRWSALDGLGRIAAARGRHQDALAAFERAMTEIEAVRSSLPRRSLRF
                     HFFGERRPVYAAAVEVLADLEARQPGAGHAARALEVVQRAKVRELLDALGRAGGAKPL
                     TAADLRRRAGDGVLLEYFLGERRLFLWTVSGGGLRMKDLGPSAPILGKAAEVHRELSR
                     GGVPAGPALAALSRALLGPAGRLPEGSRELRIAPDGALRYLPFEILEAPGDPGPPLVS
                     RVRTSYLPSGSAMAWLGHGEDRPALTLLGFGGPEIPARREGLTGAAVLEARYRLPPLP
                     ATVRELESAARRLPGRTELRLGRDATEAAFRQSMKSGTRILHLATHTVIDERPGAGTA
                     ILLTPAGDDDGLLSPAEIAGLEGSADLTVLAACSTALAPDAGSGDALATLTGSFLAAG
                     SPAVVATLWDVGDDATAVFMEQLYHQLGRGHTPAEALRRAKERLRADPRWQSPALWAG
                     YVLVGEAPAVVRRPLAWLGIWIAAALAAAGLAWILFRRRVRES"
     gene            complement(3638..4258)
                     /locus_tag="VEL74_18285"
     CDS             complement(3638..4258)
                     /locus_tag="VEL74_18285"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYG64534.1"
                     /translation="MAVAVLPAAGASRRMGRPKLLLPVGDTTLVGAVAASLRRAAVSP
                     IVLVTAPDANGGALRDWARGAGIEGLRVAVNPAPERGMLSTIREGIAALGGAADLAER
                     GEILLVTPADLPGLRPESVRQLLHFMVTADGTALAVPVHRGRRGHPLAIAPFLIPEIG
                     ELDPDVGLRQLLDRHADEVIEVEMDDPGVVEDVDTPEDYARLGCAS"
     gene            complement(4325..6577)
                     /locus_tag="VEL74_18290"
     CDS             complement(4325..6577)
                     /locus_tag="VEL74_18290"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HYG64535.1"
                     /translation="MTSHLSTATPKAARQWIPFFVLGVSLLLTAAATYYVVVTAHARD
                     GIRFQSHVQRTQAGIQTRMETYIALLHATSGLFAANGEVGREQFHNFVSPIDIRSRYP
                     GLQGIGFSRRVAADQVDALEASVRAEGLPRFEVWPESPAADRNAIVYLEPLDRRNRVA
                     MGFDMTTDPVRRAAMERARDTGMPVATGKILLVQEIDDRKQPGFLIFLPVYRQGTPVA
                     TVAQRRAALEGFVFSPFRTADLLEGIFGTGASPPISFAIYSGRTPRPDALLFHSAPGE
                     QGDDGPTFRATRLLDFAGAPWTISYATLPVFDATSGKNHAALLLVLGLFASGVLFAIA
                     QSQVEAREEAEAASQAKDHFLAALSHELRTPLTPVLAVLSGMESDDRLPADVVSNLAM
                     VRRNVELEARLIDDLLDITRITQGKLELLPRTADLEQILEHAIETCSGQVPPASRPRI
                     VTDLAPLDHRLVADAPRLTQVFWNLLSNAIKFTPADGTVRVRTSQDAERQQLVVEVSD
                     TGMGIDPERLPHIFDAFFQAGRSIARQFGGLGLGLAISKTIVELHGGTLTAVSEGPGK
                     GATFTVRLPIGDILDQTASLLTPLGKTRLEPPLTAGAAGAAETAAAGTGRPLHILLVE
                     DHADTADAMATLLRAAGRSVTVAGSVAAALEAAEAACGTGSPDGHGPIDLVVSDVGLP
                     DGTGLELMSELSRRYGLQGIALSGFGMDDDLRKSQEAGFAQHLTKPVTLQTLETAIAQ
                     VTATASAPRL"
     gene            complement(6687..>7067)
                     /locus_tag="VEL74_18295"
     CDS             complement(6687..>7067)
                     /locus_tag="VEL74_18295"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64536.1"
                     /translation="AAHCRSSFWKLRRRGSIDFAVFSAAAALWTDGTDRRVVTRARIY
                     LGAVASYPLPATAAAEFLVGKPLTEETVAEAARLARKVATPFDNTDFQPQWRNVMADR
                     YTEAALREAAGLPQQRMGPKHQAL"
BASE COUNT          971 a         2344 c         2819 g          933 t
ORIGIN      
        1 cccagtggat catgtccagc acctcctggg tgttccaggt ccagaacagc atcccttcca
       61 gcagctcctc ggggtctccc tggccttcga ggacgtactc gttcatcttg tacgtctcgg
      121 gcatgttggc ctcgatggag aagtgagtga atcccatctc ctcgaccagg aattcgagca
      181 ggcggtgttt catcaggaag aattcccgcg tgccgtgggt cgcctcgccg agggagacga
      241 tgcgggcgtc accgaaggct tgccgcagcg gctggaggtc ggcgaaaccg ttgccggccg
      301 cggcggtggc gagggggagg gcgttgccgc gcaaccacgc cgcgtgcccg ggcgtcggct
      361 cgaacaggtt cggtgcccgg tccttccagg gttggccgtc gatctcgatc cggatgtcgt
      421 cgaaccaggc cgtgccgggg ccgaccaggt cgacgccgaa gtaggtctgc tgcaggtcgg
      481 ccgggatgtc gatctcgaat tcgtagcgcg tccacggcgt cgtgcccgcg ccctcggcgc
      541 cgctgttgcc gaaggtgacg ccgcccgcct gatcgatggc gatccagtag agtccggccc
      601 agatcgggac cccctcggtg cggatcgagc cgctgaacct gatcttccgg cccgccgcct
      661 gctcgttggg gaagagctgg gcgatcgacc ccacgcccag ttgtggaacc cattggaagg
      721 ggtacacgta gccgatccgc aggctctgcc cgcccgacga ggcctgggtc tgatcgtaca
      781 gggaatcgta cgcgcgccac gtggggccca gccgcgtcca ttcgctcgta cactcgggcg
      841 cttcgaaatc cagatccaga aacggacggg gatccgcgcc ggcgggaggg atcatcagac
      901 aacagagggc cagcatgaga cacactcttc tcatcagaaa accttccttt caccacctcg
      961 gcgatggtcg aagtgaacca ggtctgcggt gccccactgc aagggccgtg caaacctgat
     1021 cgaaggcgtt ctggaggccg cggcgtcctc cccggatggc ggtcgggtcc ccgcagacgg
     1081 acgaatgggc ggacggaaag ggcagatata aaatgtgatc tatcttagga ttctcggacc
     1141 cgccgccgga agaggatcca ggccagaccg gcggccgcca gcgccgccgc gatccagatc
     1201 ccgagccacg ccagcggccg ccgcaccacg gcgggggcct cgccgaccag gacgtagccg
     1261 gcccagagcg ccgggctctg ccagcgggga tcggcgcgca gccgctcctt ggcccgccgg
     1321 agcgcctcgg ccggggtgtg gccgcggccg agctggtggt agagctgctc catgaagacg
     1381 gccgtggcgt cgtcccccac gtcccagagc gtcgcgacga cggccggcga gccggccgcg
     1441 aggaacgagc cggtgagggt ggcgagcgcg tcgcccgagc cggcgtccgg cgccagggcg
     1501 gtgctgcagg cggcgaggac ggtgaggtcg gcgctgcctt ccaggccggc gatctcggcg
     1561 ggggagagca gcccgtcgtc gtcgccggcc ggggtgagga ggatggccgt gccggcgccg
     1621 gggcgctcgt cgatcaccgt gtgggtcgcc agatggagga tgcgggtgcc gctcttcatg
     1681 gactgccgga aggcggcctc ggtggcgtcg cggcccaggc gcagctccgt gcggccgggg
     1741 agccggcggg ccgccgactc gagctcccgg accgtggccg gaaggggagg gaggcgatag
     1801 cgggcttcca ggacggccgc gccggtgagc ccttcccgcc gggccgggat ctcggggccg
     1861 ccgaagccga ggagggtcag cgccggccgg tcctcgccgt ggccgagcca ggccatggcg
     1921 gagccgctgg ggagatagct ggtcctgacc cgggagacga ggggggggcc ggggtcgccc
     1981 ggcgcctcga ggatctcgaa ggggagataa cgaagggcgc cgtccggggc gatccgcagc
     2041 tccctggagc cctcgggcag ccggccggcc gggccgagca gggcacgcga aagggccgcg
     2101 agcgccggtc ccgcgggcac gccgccccgc gacagctcgc ggtgcacctc ggcggccttg
     2161 ccgaggatgg gggcggaggg gcccaggtct ttcatccgga gccccccgcc gctcaccgtc
     2221 cacaggaaga ggcggcgctc gccgaggaag tactccagga ggacgccgtc gccggcccgc
     2281 cggcgcaggt cggcggcggt gaggggcttg gcgccgccgg cacggcccag cgcgtcgagc
     2341 agctcccgca ccttggcgcg ctggaccacc tccagggcgc gggcggcgtg gccggccccg
     2401 ggctgccggg cctcgaggtc ggccaggacc tcgacggccg cggcgtagac cggccgccgc
     2461 tcgccgaaga agtggaagcg caacgagcgg cgggggaggc tggagcgcac cgcctcgatc
     2521 tccgtcatgg cgcgctcgaa ggcggcgagg gcgtcctgat gccggccgcg ggccgccgcg
     2581 atgcggccga gcccgtccag ggccgaccag cgcccctcgg ccgagccgct ccgctcggcg
     2641 gcggccaggc agcgtctgaa gaccctctcg gcctcgccga gccggccgcg gccgaggaga
     2701 agacggccca gatagaagag gtcctggatc tcctccgagc ggtcgccgct cgcctcggcg
     2761 tagtggagcg ccttacggta ggcctcctcg gcggcgcgcg ggctctccag gttgaggtgg
     2821 tgcaccaggc cgatgttcag ccaggcgttg gatagggcgg ccacgtctcc ggcatccccc
     2881 tcgccttcga agatcgccac cgcctgccgg aactcctcga gcgcgcgcac cgggtccccc
     2941 aggttgcggt agacgacgcc ggtgtcgacc tcgagcgtgg cgcgctggcc gggctccagg
     3001 gcgtccccct cggcgagcac ctggcgatag agggcgaggg cctcctgata ggcgcccagc
     3061 ttctccagca ccaccccctg gttgatgcgg gcgacgcgga gctggacggg ggcccagggc
     3121 tcgccggcat gcgccgcggc caggcgggcg gcctcggcgt ggaaggcgag ggcgcggccg
     3181 tagcgcccgg cggaggtctc ggagagcccc aggttggcga ggttgatcac ctgggcctcg
     3241 acgtcgccgg tgcggcggtt gatcgccagg gcctcgcgga agcgctgctc ggcctcgtcc
     3301 cagcgtccga ggtattggag agagaggccg gtgttgttga gcgcccggga gaggcggagc
     3361 gggtcgtcga tcccctggaa gaggcggagc gactcctggc actcggcgag ggccgcccgg
     3421 tagtcccccc ggtcgttgag gatggcacag gcgttgttgc gggcgctggc cgcggaggcc
     3481 gggtccgagc cctggaggga gcgggccacg acacggtagg ccgccagggc ctcgtccagc
     3541 cggccggcgg cgtggagggc gcgggcgtcg tccagggtct gggcgcagag ggcggcggcg
     3601 aagagggaga gactacagcc gaggctacag ccgagggtca ggacgcgcat cccagcctcg
     3661 cgtagtcctc cggcgtgtcc acgtcctcca ccacgccggg gtcgtccatc tccacctcga
     3721 tcacctcgtc ggcgtggcgg tccaggagct gccgcaggcc gacgtccggg tcgagctcgc
     3781 cgatctcggg gatgaggaac ggggcgatgg ccagggggtg gccccgccgg ccgcggtgga
     3841 ccggcacggc gagcgccgtg ccatcggccg tgaccatgaa atggaggagc tggcgcaccg
     3901 actcggggcg gaggccgggg aggtcggcgg gggtcaccag gaggatctcg ccgcgctccg
     3961 ccaggtccgc ggcccccccc agcgcggcga tcccctcgcg gatggtggac agcatgccgc
     4021 gctcgggagc ggggttgacc gccaccctca gcccctcgat gccggcaccg cgggcccagt
     4081 cccggagcgc gccgccgttg gcgtccggcg ccgtcaccag gacgatgggg gagacggcgg
     4141 cgcggcgcag gctggcggcc acggcgccca ccagggtggt gtcgcccacc ggcagcagga
     4201 gcttggggcg gcccattcgg cgcgaggcgc cggcggcggg gaggacagcg acggccatgc
     4261 ggccaccata gcagggggtc ggcccggagg gaaagcgggg ccggcgcgat accccgggag
     4321 gggcctagag ccggggcgcg gaggccgtcg ccgtcacctg ggcgatggcg gtctccaggg
     4381 tctgcagggt cacgggcttg gtgaggtgct gggcgaaccc cgcctcctgg ctcttgcgga
     4441 ggtcgtcgtc catgccgaag ccggagaggg cgatcccctg gaggccgtag cggcgggaca
     4501 gctcgctcat cagctccagg ccggtgccgt cgggcaggcc gacgtcgctg accacgaggt
     4561 cgatggggcc gtgcccgtcc ggggagccgg tgccgcaggc cgcctcggcc gcttccaggg
     4621 cggcggccac cgagccggcc acggtgacgc tccggcccgc ggcgcggagc agggtcgcca
     4681 tggcgtcggc ggtgtcggcg tggtcctcca ccaggaggat gtggaggggc ctgcccgtgc
     4741 cggccgcggc ggtctcggcg gcccctgcgg cccctgcggt cagcggcggc tcgaggcggg
     4801 tcttaccgag gggggtgagc agggaggcgg tctggtccag gatgtcgccg atgggcagcc
     4861 gcaccgtgaa ggtggccccc ttgccggggc cttcgctcac cgccgtcagg gtgccgccgt
     4921 gcagctcgac gatggtcttg ctgatggcga ggccgagccc caggccgccg aactggcggg
     4981 caatgctcct cccggcctgg aagaaggcgt cgaagatgtg ggggaggcgc tccggatcga
     5041 tgcccatgcc ggtatccgag acctccacca cgagctgctg gcgctcggcg tcctggctcg
     5101 tccgcacgcg cacggtgccg tcggccggcg tgaacttgat ggcgttgctc agcaggttcc
     5161 agaagacctg ggtcaggcgc ggcgcgtcgg ccaccaggcg gtggtcgagg ggcgcgaggt
     5221 cggtgacgat gcgtgggcgg gaggccggag ggacctggcc cgagcaggtc tcgatggcgt
     5281 gctccaggat ctgctccagg tcggcggtcc gcgggaggag ctcgagcttg ccctgggtga
     5341 tccgggtgat gtccagcagg tcgtcgatga ggcgggcctc gagctcgacg ttgcgccgca
     5401 ccatggcgag gttggagacg acgtccgccg gcagccggtc gtcggactcc atgccggaga
     5461 ggacggcgag caccggcgtc agcggagtcc gcagctcgtg gctcagagcg gcgaggaagt
     5521 ggtccttggc ctggctggcc gcctccgcct cctcgcgcgc ctcgacctgg ctctgcgcga
     5581 tggcgaagag gacgccgctg gcgaagaggc cgaggaccag gagcagcgcc gcatggttct
     5641 tgcccgaggt cgcgtcgaag acgggcaggg tggcgtagga gatggtccag ggggcgccgg
     5701 cgaagtcgag gagccgggtc gccctgaagg tcgggccgtc gtcgccctgc tcgccgggcg
     5761 ccgagtggaa cagcagggcg tccggccgcg gcgtgcggcc ggagtagatg gcgaaggaga
     5821 tcggcgggga ggcgccggtg ccgaagatcc cctccagcag gtcggcggtg cggaaggggc
     5881 tgaagacgaa gccctccagc gccgcgcgcc gttgcgcgac ggtggcgacg ggggtgccct
     5941 gccggtagac cggcaggaag atgaggaagc cgggctgctt ccggtcgtcg atctcctgga
     6001 ccagcaggat cttgccggtg gcgacgggca tgccggtgtc gcgggcgcgc tccatggcgg
     6061 cgcggcggac cgggtcggtg gtcatgtcga agcccatggc gacgcggttg cggcggtcca
     6121 gcggctccag atagacgatg gcgttgcggt cagccgccgg cgattcgggc cagacctcga
     6181 accggggcag cccctcggcg cggaccgagg cttccagggc gtccacctgg tccgcggcga
     6241 cccggcggct gaagccgatc ccctggagtc ccgggtagcg gctccggatg tcgatggggg
     6301 agacgaagtt gtggaactgc tcgcggccga cctcgccgtt cgccgcgaag aggccgctgg
     6361 tggcgtgcag cagggcgatg taggtctcca tccgggtctg gatgccggcc tgggtccgct
     6421 gcacatggga ctggaaccgg atgccgtcgc gggcgtgggc ggtcaccacc acgtagtagg
     6481 tggccgccgc cgtcaggagg agcgacaccc cgagcacgaa aaagggtatc cactggcgcg
     6541 cggccttcgg tgtcgccgtg gaaaggtggc tcgtcatgct cggacaatcc gcagagttgc
     6601 tgtttccagc cgggacggtc atggtaccac cgtcgcaggg gggagggacc cgcgcggcgg
     6661 gccggcgaat ccggcgtgga cgccgctcag agcgcctggt gcttcgggcc catccgctgc
     6721 tggggcaggc cggcggcttc gcggagggcg gcttcggtgt agcggtcggc catcacgttc
     6781 cgccactggg gctggaagtc ggtgttgtcg aagggggtcg ccaccttgcg ggcgaggcgg
     6841 gcggcctcgg cgacggtctc ctcggtgagc ggcttgccca cgaggaactc ggcggcggcg
     6901 gtggccggca gggggtagga ggccacggcc ccgaggtaga tccgggcgcg ggtgaccacc
     6961 ctgcggtcgg tgccgtccgt ccagagggcg gcggcggcgg agaagacggc gaagtcgatc
     7021 gagccccggc ggcgcagctt ccagaaggag gagcggcagt gggcggc
//
LOCUS       DASPJI010000648         7904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_268265, whole genome shotgun sequence.
ACCESSION   DASPJI010000648 DASPJI010000000
VERSION     DASPJI010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7904)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7904
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268265"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VEL74_18300"
     CDS             <1..412
                     /locus_tag="VEL74_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64537.1"
                     /translation="PDFTGDPTPGEIIFGPSPDMIHYGGAFLAVLDPTGSTVLRSEIL
                     AENEESVRATDVAVTPDGRAYYLVMTSSYPVPGLGQFRADGGPVSYPQLSYGCGWGDW
                     ISAYLTGLATDGAGNLYVSGTEYVQDTGTSRAYV"
     gene            1124..1306
                     /locus_tag="VEL74_18305"
     CDS             1124..1306
                     /locus_tag="VEL74_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64538.1"
                     /translation="MSLRAARSGQGDGRVYHLRFTVTDESGLACTGEAAVCVPHSQGR
                     GSTCGDGGARFASGSR"
     gene            complement(1846..2181)
                     /locus_tag="VEL74_18310"
     CDS             complement(1846..2181)
                     /locus_tag="VEL74_18310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64539.1"
                     /translation="MRNAVLLLVLTVVVFLTNSPVVSAATCTATCSGTVALSCTATTC
                     TASNGSHITCNGATTTCSSASNWCACSADCIDQCAGACELSSNACRFCISNCNQNNCG
                     PQPPFISHC"
     gene            2363..4042
                     /locus_tag="VEL74_18315"
     CDS             2363..4042
                     /locus_tag="VEL74_18315"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HYG64540.1"
                     /translation="MADERETMEIDVVFVGAGPSSLAGAYHLARLIREHNEKSADKLE
                     ISIAVLEKGKDPSSHALSGAVVDPRALQELFPDDWKSAPFEGEVEREKLLWLTGGRSF
                     SLPIPPPLNNHGKYVASLGKLLKWMAPKVEAAGVDIFFEFPAARALVEDGRVVGVRTG
                     DRGVSKEGHKKANYEPGVDIRSQVVVLGEGPRGTLVKQLDGELDLWRGKNPQIYAIGI
                     KEVWEVPAGRMAPAEVIHTMGWPLGFKTFGGGFVYGMQNDQVIVGLVVGLDYENPLLE
                     PHTEFQRFKTHPDIAKLLSGGKMAFYGAKAIPEGGWWSMPQPWGDGFLIVGDSAGTLN
                     SQRLKGVHLGMKSAMLAAETIFESLKAGGVTSERLSAYSKKIDASWIREELWPVRNFH
                     QAFDHGLVAGMAQAGIGLVTGGRGWGVRDRVETTPGHTRMVRLDSPEGKHLTPPEPLK
                     MDGQLTFDRLADVYNSGTAHDEDQPVHLIVHDTSICVERCTVEFANPCQRFCPAAVYE
                     MVPDVATPSGLRLQINASNCVHCKTCDIMDPYGIITWVPPEGGDGPNYSKM"
     gene            complement(4060..5259)
                     /locus_tag="VEL74_18320"
     CDS             complement(4060..5259)
                     /locus_tag="VEL74_18320"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HYG64541.1"
                     /translation="MRQLPRFPILAAAAALALAAGTPWAGADTQTRVFRHGFPQGQGT
                     LRFANLAGRVELMPGQGPQVVVNVTVHADAGSAGETKRLLEGMKWVRGRDKEGREEWT
                     LSYPVDRYKAYHYPRENRGGSDLPGFLSFLENGYTSTTYRGERIRIYGQRRSSAPTLY
                     ADLKIALPAGSNVVMRNVVGQVDGAGALQGKLAVKTGSGSVRLASYDGQLTVDTGSGD
                     VRVGSVRGETSIDTGSGDVVVSRLIGNGLVDTGSGNVVVEKVSAGRLSIDTGSGDVTV
                     KEGIASRLLAETGSGAVRVVGVDIEELEADTGSGDVRIESSLAKARRVVADTGSGDIH
                     ILAGANASFDVESSQGSGDLKVGYADAKLRKSGNKVVGARRGDGQTRIRVETGSGDCV
                     ISPRGGE"
     gene            5363..7606
                     /locus_tag="VEL74_18325"
     CDS             5363..7606
                     /locus_tag="VEL74_18325"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYG64542.1"
                     /translation="MRLVPLPLLLCGLAALMPETASAQTQRPDPKKLTTEAVFATDAA
                     GRRASDLAWSPDGTRLAYLWDDGAGKALWILDPATGRRTTVPGSVRPARTADQAPAAP
                     AIDDFQWSPNGVSLLVEAGGDLFLHPAAGGPVRRLTETKAAETLPRFSPNGNRLAFVR
                     DNDLYMIDLDKGGRESRLTTGGEHNVTLNGIADWVYWEEIWDRETTGFWWSPDSTRIA
                     YYQFDETPVGVYPLTVEADPAVPARADFQKYPRPGEPLPKVRVGVLDLPSGRTTWTAT
                     GEEDSYLARVDWTPDGRAVAIQRLNRAQNRIDLLRCDAVSGDCSTLHTQSWPTWVNLG
                     NDLRFLSDGRFVWGSEQSGWRRLYLHDAEGKIVRPLTPEGWAVTSLDAVADNGEWLIF
                     TAFRTEGTPAVNAAERQVMRVSLNEGTAAEPLTREPGWHEANVASASGLWVHTWSDGD
                     TPHRAEVRGRDGQPVADLPSTPPRFDPAALPRWKLGTIAGPEGSRLPFRYLEPEGFNP
                     ARRYPVVVYHYGGPGSQVVVNRWDTRGRDLWHKMMAQRGFVVAMVDNRSSLFFGKAGE
                     DRDHRAMGKVNLAAQLALVDHLKAQPWADAGRIGLWGWSGGGFNTLYCLFHRPGVWKA
                     GVAGAPVTDWRLYDAIWTERYLGTPKDNPQGYRASSPFHNAEKLRDRLLVVHGLADDN
                     VHPQNTVALSDRLVRAGLPFEQAFYPGQKHGFRGESQTHFFARMTEFFERTLQQVVVV
                     DVEAEVR"
     gene            complement(7638..>7904)
                     /locus_tag="VEL74_18330"
     CDS             complement(7638..>7904)
                     /locus_tag="VEL74_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016986028.1"
                     /note="FabF; beta-ketoacyl-ACP synthase II, KASII;
                     catalyzes a condensation reaction in fatty acid
                     biosynthesis: addition of an acyl acceptor of two carbons
                     from malonyl-ACP; required for the elongation of
                     short-chain unsaturated acyl-ACP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HYG64543.1"
                     /translation="AYKLAVSSTKSATGHLLGAAGGLESGILALAIKHQTLPPTLNLD
                     EPGEGCDLDFVPHKARPADLGYAITNSFGFGGTNGAVVMKRFEG"
BASE COUNT         1233 a         2787 c         2691 g         1193 t
ORIGIN      
        1 cccggacttc accggcgatc cgacaccggg cgagatcatc ttcgggccgt cgccggacat
       61 gatccattac ggcggcgcat tcctggctgt gctcgacccc accggctcga cggtccttcg
      121 ctcggaaatc ctcgccgaga acgaggagag cgtgagggcc acggatgtcg cggtaacgcc
      181 cgatggccgt gcctattatc tcgtcatgac cagctcctac cccgtccccg gccttggcca
      241 gttcagggcg gatggagggc cggtgtccta cccgcaactg tcgtacggct gcggttgggg
      301 cgattggatt tcggcctacc tgaccgggct cgcgaccgat ggcgccggga atctctatgt
      361 ctccggcacg gaatatgtac aggacacggg gaccagccgg gcctacgtct aaagttcgat
      421 ccccgtggaa cgcagcttta cacgacctgc ctcggctccg aggagcacgt cctggggaat
      481 gacgtcgccg ccgacccggc gggcaacgct tacctgaccg ggcatgctac tggccataac
      541 ctgcctgcgg ctggagcgct acagcccgga gggggcgggg acgccttcat cgccaagctg
      601 gggcccacgg gcgaggtggt ctactcgacg tacctcggag gcagcggctt cgacgaaggg
      661 tttggcattg ccgccgatcc ctcggggtcg gtgcacgtgc agggggtcac ctactcgtcc
      721 gacttcccga aggagcgccc gctacccgac gggtgcgtct ccaccacgcg gtggagcccc
      781 tgccggttcg tggcacgcct tgggcccacg ggggagaagc tcgacttttc gaccctgttc
      841 gggccgcccg ggttcacgag caccgaggcc ggcagtaagc cgatcgccgc cgggccccgg
      901 ggtgagacct acctgatcga tggaggctcg ctcgtccgct tcgtcgccaa tcgcccgccc
      961 gactgcgccg ccgccgcggt gagcccggcc gtgatctggc cccccaacgg ccagatgaca
     1021 ccggtcgcga tcaccggcgg tcgagctgag cgtcaccgct attttccagg acgagccgag
     1081 caccccgggc acggccgacg cgaccggcat cggcacaccg aacgtgtccc tgcgcgccgc
     1141 gcgctccggc cagggcgacg gtcgcgtcta ccatctgcgc ttcaccgtca ccgacgagtc
     1201 ggggcttgcc tgcaccggcg aggcggccgt ctgcgtgccg catagccagg gccgcggcag
     1261 cacctgcggc gacggcggcg cgcggttcgc ctcgggctcg cgctagcact gggaatctga
     1321 gcgatgagcc gccgtgggcg cggacgcccc cggcggcggg taggtcggcg tcagacttcg
     1381 aggtaatcaa aggcgccttc ggggttccgg gtcacgcgga gcacggtcca gagacgctcg
     1441 gggggcgcct ccccagagga ggcccaaccc tggccgctcg acaccaatta ggtaacagag
     1501 agcaccgaag aagctcgatg gtgtcgaaac gagctggatt cgtcacctat gcccagatgg
     1561 agacacctcc tgcctgggac ttcgacacct agggctctgc attggtgtcg agatgaggcc
     1621 tcgggaggca gcgacgaggt gtgggagctt cccagtggag gatctcctgg gcagggctga
     1681 ggtgtcgaaa gaccatcatg gaaggcaccg ctccgtggat gggacaccta tggagggagc
     1741 ggaggctccc aaaggcacgc ggcaacctct ggagggtagg gccgccggag tggtcccccg
     1801 gcggcgggat gcgtgattgg agcccgtgaa gcccgggcga tcgactcagc agtgggagat
     1861 gaacggcggc tgcggcccgc agttgttctg gttgcagttg gagatgcaga accggcaggc
     1921 gttggagctc agctcacagg cgccggcgca ctggtcgatg cagtccgcgg agcacgcgca
     1981 ccaattggac gcgctgctac aagtggtggt ggcgccgttg caggtgatgt gagagccgtt
     2041 cgaggccgta caggtcgtgg cggtacagct cagggcaacg gtgcccgaac aggtcgcggt
     2101 gcacgtggcg gcggacacca ccggggagtt ggtcaggaaa acgacgacag tcagaacgag
     2161 caggagcaca gcgtttctca tctctattct cctttgttgt cgactacttc attgacagcc
     2221 ccagggtacc gccgggagaa aagagcctct atcagaatct tgctgtggcg cctggcccgg
     2281 ggccgccggc cgctctgaac atgcccgtgg tagcatccga cgggtttgcg ggcgaccgcc
     2341 ctttctggag gaccctgatc gcatggctga cgagcgcgag acgatggaga tcgacgttgt
     2401 attcgtgggg gccggccctt ccagcctggc cggcgcctat cacttggcgc gcctgatccg
     2461 cgagcacaac gagaaatctg cggacaagct ggagatctcc atcgccgtcc tggagaaggg
     2521 caaggacccc agcagccacg ccctgtccgg cgccgtcgtc gatccccgcg cgctccagga
     2581 gctgttcccg gacgattgga agagcgcgcc gttcgagggg gaggtggaac gcgagaagct
     2641 cctctggctc accggcggcc ggtcgttttc gctgcccatc ccgccgccgc tcaacaacca
     2701 cggcaagtac gtcgcctccc tgggcaagct cctcaagtgg atggctccca aggtcgaggc
     2761 cgccggggtc gacatcttct tcgagttccc cgccgcccgg gcgctggtgg aggacggccg
     2821 cgtcgtcggc gtgcgcacgg gcgaccgcgg cgtctccaag gagggccaca agaaggccaa
     2881 ctacgagccg ggggtggata tccgctccca ggtggtggtg ctgggtgagg gcccgcgcgg
     2941 cacgctggtc aagcagctcg acggcgagct cgacctctgg cgcggcaaga acccgcagat
     3001 ctacgcgatc ggcatcaagg aggtctggga ggtgccggcg ggacggatgg cgccggccga
     3061 ggtgatccac accatgggct ggccgctcgg attcaagacc ttcggcggcg gcttcgtcta
     3121 cggcatgcag aacgaccagg tcatcgtcgg cctggtcgtg gggctcgact acgagaaccc
     3181 gctgctcgag ccgcacaccg agttccagcg cttcaagacc catccggaca tcgccaagct
     3241 cctttcgggg ggcaagatgg cgttctatgg cgccaaggcg atcccggagg ggggctggtg
     3301 gtcgatgccg cagccctggg gggacggctt cctcatcgtc ggcgactcgg cgggcacgct
     3361 caacagccag cgcctcaagg gggtccacct cgggatgaag tcggcgatgc tggccgccga
     3421 gacgatcttc gagagcctca aggcaggcgg cgtcacctcc gagcggctct cggcctactc
     3481 gaaaaagatc gacgcgagct ggatccggga ggagctctgg ccggtgcgca atttccacca
     3541 ggctttcgac cacggcctcg tggccggcat ggcgcaggcc ggcatcggcc tggtaaccgg
     3601 cggccggggc tggggtgtcc gcgaccgcgt cgagacgacg cccggccaca cccggatggt
     3661 gcggctggac agcccggagg ggaagcacct cactccgccc gagccgctca agatggacgg
     3721 ccagctcacc ttcgaccgtc tggccgacgt ctacaactcc ggcaccgccc acgacgagga
     3781 ccagccggtc catctgatcg tgcacgacac cagcatctgc gtcgagcgct gcaccgtgga
     3841 gttcgccaac ccctgccagc gcttctgtcc cgccgcggtc tacgagatgg tccccgacgt
     3901 cgccaccccc tcgggcctcc ggctgcagat caacgccagc aactgcgtcc actgcaagac
     3961 ctgcgacatc atggacccct acggcatcat cacctgggtg ccgcccgagg ggggcgacgg
     4021 gccgaactac agcaagatgt agcccggccc cgcttcttcc tactccccgc cccgcggcga
     4081 gatcacgcag tcgccgctgc cggtctcgac gcggatccgg gtctggccgt cgccgcgccg
     4141 ggcgccgacg accttgttgc cggacttccg gagcttggcg tccgcgtagc cgaccttgag
     4201 gtcgccgctg ccctgggagg actcgacgtc gaaggaggcg ttcgcgccgg cgaggatgtg
     4261 gatgtcgccg ctgccggtgt cggccacgac gcgccgggcc ttggcgagcg agctttcgat
     4321 gcggacgtcg cccgagccgg tgtccgcctc caactcctcg atgtccaccc cgaccacccg
     4381 caccgccccg gagccggtct cggccagaag ccggctcgcg atcccctcct tcaccgtcac
     4441 gtcgcccgag ccggtgtcga tcgacaggcg cccggccgag accttctcga ccacgacgtt
     4501 gcccgagccg gtgtccacca ggccgttgcc gatgagccgg ctcaccacca cgtcgcccga
     4561 gccggtgtcg atcgaggtct cgccacggac cgagccgacg cggacgtcgc ccgagccggt
     4621 gtccaccgtg agctggccgt cataggaggc gagccggacg ctgccgctgc cggtcttcac
     4681 cgccagcttg ccctgcaggg caccggcgcc gtccacctgc ccgacgacgt tgcgcatcac
     4741 cacgttgctg ccggcgggca gcgcgatctt gaggtcggcg tacagggtgg gggccgagga
     4801 cctcctctgg ccgtagatgc ggatccgctc gccccggtag gtcgtcgagg tgtagccgtt
     4861 ctccaggaag ctcaggaagc cgggcaggtc ggagccgccg cggttctcgc gcggatagtg
     4921 gtaggccttg tagcggtcta ccggatagga gagggtccac tcctccctcc cctccttgtc
     4981 gcgcccccgc acccacttca tcccctcgag caggcgcttg gtctcgccgg cgctgcccgc
     5041 gtcggcgtgg acggtgacgt tcaccaccac ctgggggccc tggccgggca tcagctcgac
     5101 gcggccggcg aggttggcga agcgcaacgt gccctgcccc tgggggaagc cgtgacggaa
     5161 gacccgggtc tgggtatcgg cacccgccca cggggtgccc gccgccaggg cgagcgccgc
     5221 cgcggcggcc aggatcggaa atctgggaag ctgtctcatg gtcgttctcc ttgtcctctc
     5281 actggccact acgggacgcg cggaaagaag gttgcaaccg ggtaaacttc ccgggcccgg
     5341 aacccatctt ggaggatatc ccatgcgtct cgtccccctc ccgctgctcc tctgcgggct
     5401 cgccgccctg atgccggaga ccgcgtccgc ccagacccag cggccggacc ccaagaagct
     5461 cacgaccgaa gcggtcttcg ccaccgacgc cgccggccgc cgcgcctccg acctcgcctg
     5521 gtcgccggac ggcacccgcc tcgcctacct ttgggatgac ggcgccggca aggcgctctg
     5581 gatcctcgac cccgcgaccg gccggcgcac caccgtgccg ggcagcgtcc ggccggcgcg
     5641 gacggccgac caggccccgg ccgcgccggc gatcgacgac ttccagtggt ccccgaacgg
     5701 tgtctccctg ctggtggagg cgggcggtga cctgttcctc cacccggcgg ccggcgggcc
     5761 cgtccggcgg ctgaccgaga ccaaggccgc ggagaccctc ccccgcttct cccccaacgg
     5821 caaccggctc gccttcgtcc gcgacaacga cctctacatg atcgacctgg ataagggggg
     5881 acgcgagagc cgcctcacca ccggcggcga gcacaacgtc accctgaacg gcatcgccga
     5941 ctgggtctac tgggaggaga tctgggaccg cgagaccacc ggcttctggt ggagccccga
     6001 cagcacgcgc atcgcctatt accagttcga cgagaccccg gtcggcgtct acccgctgac
     6061 cgtcgaggcc gaccccgccg tgcccgccag ggccgacttc cagaagtacc cgcggccggg
     6121 agagcccctc cccaaggtgc gcgtcggcgt cctcgatctc cccagcggcc gcaccacctg
     6181 gacggccacc ggtgaggagg actcctatct agcccgggtg gactggacgc cggacggccg
     6241 cgccgtggcc atccagcgcc tgaaccgggc ccagaaccgc atcgacctcc tgcgctgcga
     6301 cgccgtctcg ggcgactgct ccaccctcca cacccagagc tggcccacct gggtgaacct
     6361 gggcaacgac ctccgcttcc tgagcgacgg ccgcttcgtc tggggctccg agcagagcgg
     6421 ctggcgccgg ctctatctcc atgacgccga aggcaagatc gtccgccccc tgactccgga
     6481 gggctgggcg gtcacctcgc tcgacgccgt ggctgacaac ggcgaatggc tgatcttcac
     6541 cgcgtttcgg actgaaggga ccccggcggt caacgccgcc gagcggcagg tgatgcgcgt
     6601 cagcctcaac gaagggacgg ccgcggagcc gctgacccgc gagcccggct ggcacgaggc
     6661 gaacgtcgcc tccgcgagcg gcctctgggt ccacacctgg agcgacggcg acacgccgca
     6721 ccgcgccgag gtgcggggcc gcgatggcca gccggtggct gacctcccct ccacaccacc
     6781 ccgcttcgac ccggccgcgc tgccgcgctg gaagctcggc accatcgccg gcccggaggg
     6841 ctcgcgcctc cccttccggt atctcgagcc ggagggtttc aatccggcgc gacgctaccc
     6901 ggtggtcgtc tatcactatg gcgggccggg ctcacaggtg gtggtgaacc gctgggatac
     6961 ccgtggacgc gacctctggc acaagatgat ggcgcagcgc ggcttcgtcg tcgccatggt
     7021 ggacaaccgg tcgtcgctgt tcttcggcaa ggccggagaa gaccgcgacc atcgcgccat
     7081 ggggaaggtg aacctggccg cccagctcgc cctggtggac cacctgaagg cgcagccctg
     7141 ggcggacgcc ggccgcattg gtctctgggg ctggtcgggc ggcggcttca acacgctgta
     7201 ttgcctgttc catcgccccg gcgtctggaa agccggagtc gccggcgccc cggtgaccga
     7261 ctggcgcctc tacgacgcca tctggaccga gcgctatctc ggcacgccga aggacaaccc
     7321 ccagggctac cgcgcctcct cacccttcca caacgccgag aagctccgcg accgcctgct
     7381 ggtcgtccac gggctcgccg acgacaacgt ccatccgcag aacacggtgg cgttgagcga
     7441 ccggctggtc cgcgccggcc tgccgttcga gcaggccttc taccccggcc agaagcacgg
     7501 cttccgcggc gagtcgcaga cccacttctt cgcccggatg accgagttct tcgagcggac
     7561 gctacagcag gtggtggtgg tggacgtgga ggcggaggtg aggtaggacc tcacctccgc
     7621 cggaggaggg tgggggacta cccctcgaag cgcttcatca ccacggcgcc gttggtgccg
     7681 ccgaagccga aggagttggt gatggcgtat ccgaggtcag ccgggcgggc cttgtggggg
     7741 acgaagtcga ggtcgcaccc ctcgcccggc tcgtcgaggt tcagggtggg cgggagcgtc
     7801 tggtgcttga tggcgagggc caggatgccc gactccagac cgcccgccgc tcccagcaga
     7861 tgcccggtgg cactcttggt ggacgagacc gccagcttgt aggc
//
LOCUS       DASPJI010000649         5526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_42057, whole genome shotgun sequence.
ACCESSION   DASPJI010000649 DASPJI010000000
VERSION     DASPJI010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42057"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VEL74_18335"
     CDS             complement(<1..1074)
                     /locus_tag="VEL74_18335"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYG64544.1"
                     /translation="MNELRQDAKLAPQLVHSAYLPGSPSQWGELDPPLPEPIARALGR
                     GGVPRLWSHQAEGIAAVRRGENVLITTPTASGKSLVFQLPALEEAIRGGPGHGLFLFP
                     LKALGQDQRGKFRQLAAAAGLSEEQAGCEIYDGDTPAALRTAIRKSFPRVVVSNPDML
                     HLGILGHWTGWGPFLADLRWIVLDELHTYRGIFGSHFHHVLQRLLRLCRSVGSDPVLI
                     ASSATAANAGEFAQALTGRPFHWITESGAPREGRHLLLFRPEASPYTLALNLFVRLVR
                     AGLKTIVFTKARRITELLYSWLRREEPALAGRVASYRAGFLAEERRRIEKSLFEGKLD
                     GVISTSALEMGIDVGGLDACILVG"
     gene            complement(1171..1794)
                     /locus_tag="VEL74_18340"
     CDS             complement(1171..1794)
                     /locus_tag="VEL74_18340"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HYG64545.1"
                     /translation="MSVEEHLQPGEQVVYQAHVSRISLNPWVILAVALVVGGVIAGQM
                     APDPIVTGLALIPAAAVGLLVLGKWVVLRAHEYVLTDRRVIQQTGIVSKRSVDSRLDK
                     INNVEHQQTLWGRLLGYGDVWIDTASETGTTMFRQIAEPLEFKRAILGASESYRTWSQ
                     APVAPVAGPPPRLTGAARLRELKALLDEGLISEAEFEAKRRELVSEL"
     gene            1901..3397
                     /locus_tag="VEL74_18345"
     CDS             1901..3397
                     /locus_tag="VEL74_18345"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HYG64546.1"
                     /translation="MSPPNRRLLVVSNRLPIVLQRKDSNWILKPGSGGLVSALAPVLS
                     HRGGRWIGWPGMALEKGGAWKEVLAEGYRERGYELVPVLLGEEEVKGFYEGFSNAILW
                     PLFHDLVGRCDFEPSFWYSYLDVNRKFAECVLEHSDDDDFVWVQDYQLIHVAQFVREK
                     AEQRRIGFFLHIPFPPYDIFLKLPWRGQILAAMLDYDLIGFQTQRDTRNFLTCVEHLL
                     PGLEIRDGNPISEVRSDSRVVRVGSFPIGIDYKSFEETARSEEVETRIQDLHRKIGSY
                     DLIVGIDRLDYTKGLPERLEAFRSAIERYPEMRERVVFFQIVVPSREAVPEYQTLKGH
                     IERLVGEINGEYSTAGWVPVQYHYKSLPRRDLVSLYRMARTCFVTSLKDGMNLVAKEF
                     CACQIDGGGVLVLSEFAGAASQLQEGALLVNPHDIEGMADALLEAFTMEEDERRHRMR
                     SMQQVLRQQDIFWWVDYYLTAALGTVPEDFLTPTEYVPPMDLDERWIG"
     gene            3394..4245
                     /gene="otsB"
                     /locus_tag="VEL74_18350"
     CDS             3394..4245
                     /gene="otsB"
                     /locus_tag="VEL74_18350"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HYG64547.1"
                     /translation="MSPPKSDDSGEARPSALECWNEIADRLAGSKPAVFLDYDGTLSP
                     IAPRPELATLPLATREVLQHLALHLPVAILSGRGLEDVTALVGISSIIYAGSHGFDIS
                     GPPLAEGGPPLRYEVGEGIPERIERAAARLRRELQGIEGVLVEPKRFSVAVHFRLADE
                     AALPRIEQAVDATLADAADAADIAGLRKAHGKKVFELRPDLDWDKGKALLWLLEMRLD
                     HAANPVVPLYIGDDVTDEDAFRVLEERGIGILVAEEPRPTAAAYSLRDPDEVRQLLER
                     LADCTKS"
     gene            complement(4557..>5526)
                     /locus_tag="VEL74_18355"
     CDS             complement(4557..>5526)
                     /locus_tag="VEL74_18355"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64548.1"
                     /translation="WSFQTKLMPSDPATFKDFGFSVALSADGTRALIGAPRGETCPDL
                     GISCGAAYAFVESGGVWMEEDKLRQSDPEMTEFFGSSVALSADAGIALIGATFDRCGP
                     QSCGAAYVFFRGGAVWTEQQKLTPTVPEPGSGFGTSVALSATGDTALIGAPGYPPTVL
                     PAGPEPGLFYGAAFVFQRSGGTWLEQHAFLDLGGSAGTSVDLSESGDVALIGAPGDFL
                     VDPDIPGWAGAAQRSGGVWGVPVPLVSSSSQIGDGFGNGVALSADATTALAGAPGEDV
                     SGIESGASHVFVIAAAVVEVPTAGEWGLLALALLTALAGTAVLRRF"
BASE COUNT          870 a         1936 c         1910 g          810 t
ORIGIN      
        1 gcccaccagg atgcaggcgt cgagcccgcc gacgtcgatc cccatctcga gcgccgaggt
       61 ggagatcacc ccgtccagct tgccctcgaa gagcgacttc tcgatgcgcc gccgctcctc
      121 ggcgaggaag ccggcgcggt agctcgccac ccggccggcc agcgccggct cctcgcgccg
      181 caaccaggag tagagcagct cggtgatccg ccgggccttg gtgaagacga tggtcttgag
      241 gcccgcccgc accagccgca cgaacaggtt gagcgccagg gtgtaggggc tcgcctccgg
      301 cctgaagagc agcaggtggc gcccctcgcg cggcgctccg gactcggtga tccagtggaa
      361 gggccggccg gtcagggcct gggcgaactc cccggcgttg gcggcggtgg ccgaggaggc
      421 gatgagcacc ggatcgctcc ccaccgagcg gcagaggcgg agcaggcgct gcagcacgtg
      481 atggaagtgg ctgccgaaga tcccccggta ggtgtgcagc tcgtcgagga cgatccagcg
      541 caggtcggcg aggaacggcc cccagccggt ccagtgcccc aggatcccca ggtggagcat
      601 gtccgggttc gacaccacca cccgcgggaa gctcttgcgg atcgcggtgc gcagggcggc
      661 cggggtgtcg ccgtcgtaga tctcgcaccc cgcctgctcc tcgctgaggc cggccgccgc
      721 ggcgagctgc cggaacttgc cccgctggtc ctgccccagc gccttgagcg ggaagaggaa
      781 cagcccgtgc cccggcccgc cgcggatcgc ctcctccagg gccgggagct ggaacaccag
      841 cgacttgccc gaggccgtcg gcgtggtgat gaggacgttc tcgccccgcc gcacggccgc
      901 gatcccctcg gcctggtggc tccacagccg cggcaccccg ccccggccca gggcccgggc
      961 gatgggctcg ggcagcggcg ggtccagctc cccccactgg gacgggctcc ccggcaggta
     1021 ggcggagtgg acgagctggg gcgcgagctt cgcgtcctgg cggagctcat tcaccagatg
     1081 gctcagagag tcgatcgcga ggggcacggc ggttactgct gaaatattac gcggcactcg
     1141 cgccggatct taccaggctc gggcggcgct tcaaagctcc gagaccagct cccgccgctt
     1201 cgcctcgaac tcggcctcgc tgatcagccc ctcgtcgagg agcgccttga gctcgcggag
     1261 gcgggcggcg ccggtgagcc gcggcggcgg cccggcgacg ggagcgaccg gagcctggct
     1321 ccacgtccgg taggactcgg aggcgccgag gatggcgcgc ttgaactcca gcggctccgc
     1381 gatctgccgg aacatggtcg tgccggtctc gctcgccgtg tcgatccaga cgtcgccgta
     1441 gcccagaagc ctcccccaga gcgtctgctg gtgctcgaca ttgttgatct tgtcgagccg
     1501 ggagtccacc gaccgcttgc tcacgatccc ggtctgctgg atgacgcggc ggtcggtgag
     1561 gacgtactcg tgagcgcgga ggaccaccca tttcccgagg accaggaggc ccacggccgc
     1621 ggccggaatc agagccagac ctgtaacgat ggggtccggc gccatctgcc cggcgatcac
     1681 cccgccaacc accagggcca ccgccaggat cacccagggg ttcagggaga tacggctcac
     1741 gtgggcctgg tagacgacct gctcaccggg ctggagatgc tcttcgacgc tcatcgctcg
     1801 ctcctccgcg ggctcctcaa tgtacagggc caaggggaga tgctatcgca cccaagattc
     1861 cggcgctctg gtttccggct tgcattagac tatcgatgct ttgagccctc cgaaccgccg
     1921 cctcctcgtc gtctccaacc gcctcccgat cgtacttcag cggaaagact cgaattggat
     1981 cctgaaaccc ggctccggcg ggctggtgag cgccctggcg ccggtgctgt cgcaccgcgg
     2041 ggggcgctgg atcggctggc ccggcatggc cctggagaag gggggcgcgt ggaaggaggt
     2101 gctggccgag ggctaccgcg agcgcggcta cgagctggtg ccggtcctgc tcggcgagga
     2161 ggaggtcaag gggttctatg aggggttctc caacgccatc ctctggccgc tgttccatga
     2221 cctcgtgggc cgctgcgact tcgagccgtc gttctggtac tcctacctgg atgtcaaccg
     2281 caagttcgcc gaatgcgtcc tcgagcacag cgacgacgac gatttcgtct gggtccagga
     2341 ttatcagctc atccacgtcg cccagttcgt gcgcgagaag gcggagcagc gccggatcgg
     2401 cttcttcctc cacatcccct tcccgcccta tgacatcttc ctcaagctcc cctggcgggg
     2461 gcagatcctg gcggcgatgc tggactacga cctcatcggc ttccagacgc agcgcgatac
     2521 ccgcaacttc ctgacctgcg tcgagcacct gctgcccggc ctggagatcc gggacggcaa
     2581 cccgatctcc gaggtgcggt ccgactcgcg ggtggtgcgc gtcggctctt tccccatcgg
     2641 catcgactac aagtcgttcg aggagacggc gcgctccgag gaggtggaga cgcggatcca
     2701 ggacctgcac cgcaagatcg gctcctatga cctgatcgtg ggcatcgacc gcctcgacta
     2761 caccaagggg cttcccgagc ggctggaggc gttccgcagc gccatcgagc gctatcccga
     2821 gatgcgcgag cgggtggtct tcttccagat cgtggtgccc agccgcgagg cggtccccga
     2881 gtaccagacc ctcaaggggc acatcgagcg cctggtgggc gagatcaacg gcgagtactc
     2941 caccgccggc tgggtgccgg tccaatacca ttacaagagc ctgccccgcc gcgacctggt
     3001 gtcgctctat cgcatggccc ggacctgctt cgtcacctcg ctcaaggacg gcatgaacct
     3061 ggtggccaag gagttctgtg cctgccagat cgacggcggc ggcgtcctcg tcctgtccga
     3121 gttcgccggc gccgcgtcgc agctccagga gggggcgctg ctggtcaacc cgcacgacat
     3181 cgaggggatg gccgacgccc tcctcgaggc gttcaccatg gaagaggacg agcgccggca
     3241 ccggatgcgg agcatgcagc aggtgctgcg ccagcaggac atcttctggt gggtggacta
     3301 ttatctgacg gcggcactgg gtaccgtgcc cgaagacttc ctgaccccga cggagtacgt
     3361 cccccccatg gacctcgacg agcgctggat cggatgagcc cccccaagag cgacgacagc
     3421 ggcgaagcgc gcccctcggc gctggagtgc tggaacgaga tcgccgaccg actggcgggc
     3481 tccaagcccg cggtcttcct cgactacgac ggcaccctct ccccgatcgc gccgcgcccc
     3541 gagctcgcca cgctgccgct ggcgacccgc gaggtgctgc agcacctcgc cctccacctc
     3601 cccgtggcga tcctctccgg ccgcgggctc gaggacgtga ccgccctggt aggcatctcg
     3661 tccatcatct acgccggcag ccacggcttc gacatctccg gcccgcctct cgccgagggc
     3721 gggccgccgt tgcggtacga ggtgggcgag ggtatccccg agcggatcga gcgcgccgcc
     3781 gcgcggctgc gccgggagct ccagggtatc gaaggggtgc tggtggagcc caagcgcttc
     3841 tccgtggctg tccatttccg gctggcggac gaggccgccc tgccccgcat cgagcaggcc
     3901 gtggacgcca ccctcgccga cgccgccgac gccgccgata tcgccggcct gcgcaaggcg
     3961 cacggcaaga aggtcttcga gctccgcccc gacctcgact gggacaaagg gaaggctctg
     4021 ctgtggctgc tcgagatgag actcgaccac gccgccaacc ccgtcgtccc cctctacatc
     4081 ggcgacgacg tcaccgacga ggacgccttc cgggtgctgg aggagcgggg gatcggcatc
     4141 ctggtggccg aggagccgcg ccccacggcg gcggcctact cgttgcgcga tcccgacgag
     4201 gtccgccagc tcctcgaacg cctggcggat tgcacaaaaa gttgacattt cctccggctt
     4261 gtccgaccat tctcctgtcc ttgatgttcc acgcgctccc gcctggggcg acgtggcggc
     4321 tccgacgggc ttgggcggcc caaggagctg ggcgggctgg gacctgccga tgatggacac
     4381 gcccaggaag gaggtggtcc agtgaacgat ggtaaacacg gtttgatgga ggtggcgtcc
     4441 tcgtaaaggg gatcctccac gccgttgacg gagggtgaac cccgggaggg gaacccaacg
     4501 ccaccgaaaa cagacctcga aggccccggt ccgcaaggac cggggccttt tagtcttcag
     4561 aagcggcgca ggaccgccgt tccggcgagc gcggtgagga gagcgagcgc caggaggccc
     4621 cactcaccgg ccgtgggaac ctcgacgacg gcggcagcga tgacgaacac atgggaggcg
     4681 ccgctctcga tccccgatac gtcctcccca ggggcgccgg ccagggccgt cgtcgcgtcg
     4741 gccgagaggg cgacgccgtt gccgaagccg tcaccgatct ggctgctgga cgacaccagc
     4801 gggaccggga cgccccagac gccgccggag cgctgagcgg ctccggccca gccggggatg
     4861 tcgggatcga cgaggaagtc ccccggcgcg ccgatcaggg ccacgtcgcc gctctccgac
     4921 aggtcgaccg acgtgccggc gctgccgcct agatcgagaa acgcatgctg ctcgagccag
     4981 gtgccgcccg aacgctggaa gacgaaggcg gcgccgtaga agaggccggg ctcgggcccc
     5041 gccggcagga ccgtcggggg gtagcccggg gcgccgatga gcgccgtgtc gccggtcgcg
     5101 gacagcgcga cggaggtgcc gaacccggag ccgggctccg gcaccgtggg agtgagcttc
     5161 tgttgctccg tccagaccgc gccgccgcgg aagaagacgt aggccgcgcc gcagctctgc
     5221 ggaccacagc ggtcgaaggt cgcgccgatc agggcgatgc ccgcgtcggc cgaaagggcc
     5281 accgagctgc cgaaaaactc ggtcatctcg ggatcggact gccggagctt gtcctcctcc
     5341 atccagacgc cgccgctctc aacgaaggcg taggcagcgc cgcaggagat gccgaggtcc
     5401 gggcaagtct cccctcgggg ggccccgatc agggcgcggg tgccgtccgc cgagagcgcc
     5461 accgagaagc cgaagtcctt gaacgtcgcc ggatcgctcg gcatgagctt ggtctggaag
     5521 ctccag
//
LOCUS       DASPJI010000650         7023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_388848, whole genome shotgun sequence.
ACCESSION   DASPJI010000650 DASPJI010000000
VERSION     DASPJI010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388848"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..481
                     /locus_tag="VEL74_18360"
     CDS             224..481
                     /locus_tag="VEL74_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HYG64549.1"
                     /translation="MQTTLRLDDELYRRAKAQAAAQGRSLTTFVEEAIREHLNRPARV
                     ARQRPLRLPVSTATGGLAPGFSSLEEAVDAADLAADRHLAG"
     gene            485..925
                     /locus_tag="VEL74_18365"
     CDS             485..925
                     /locus_tag="VEL74_18365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HYG64550.1"
                     /translation="MDLLDANILIGAFRRDDPDHGAVKGWLEESLSQGSLITFPPLVE
                     VAFLRIVTHPRIFATPSPFEEAMEFLQILQESGQLRETPWTVRMRAVWWRWCRDLELV
                     GNDVNDAYLAATATEARCRLVTRDGGFSRFPGLDWWNPIGLPEK"
     gene            complement(937..1599)
                     /locus_tag="VEL74_18370"
     CDS             complement(937..1599)
                     /locus_tag="VEL74_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HYG64551.1"
                     /translation="MPTSTVEDYLKCILLEEQREPDSLVSMRRISSALQLAPGTVTAM
                     MKTLADSGLVTYEPYSGVRLTPSGNQLAMHVLRRHRLIELFLVQVMGMDWTEVHAEAE
                     RLEHAVSDRLIERMDEMLGYPSVDPHGDPIPNIHGEMEVPDLPSLLTCGLEAPLRVAR
                     VGDQSRDFLQLVERRGLRPGNALRVLSRDEAADAVELRLESGEPLTLGFRAASKIHVE
                     PA"
     gene            1675..2622
                     /locus_tag="VEL74_18375"
     CDS             1675..2622
                     /locus_tag="VEL74_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HYG64552.1"
                     /translation="MPKILRLLPLLLLLPALLVLAACDGQGGAASSDGDGDRKLRVVA
                     TTGIIADTAARVAGSHAEVEGLMGPGVDPHLYKASESDVRRLAEADLVLYNGLHLEGK
                     MGDILGKMGRNRPVVAVAEDIPKESLRTPPEFQGQPDPHVWFDVSLWAKTLGPIARAL
                     SELDLGNAADYQANAAAYEKELLELDAWVAEEVARIPEGQRVLVTAHDAFGYFGRRYG
                     VDVIGLQGISTLSEAGLNDVDRVVQIVLDRRVKAIFVESSVPRRSIEAVQAAAASRGH
                     QVRIGGQLFSDSLGGPATPAATYPGMVRVNVETIVKALR"
     gene            2652..3527
                     /locus_tag="VEL74_18380"
     CDS             2652..3527
                     /locus_tag="VEL74_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HYG64553.1"
                     /translation="MSSAPVLESRFPPTGPAAEPAAGVPAVEVHDLTVAYRTQPVLWD
                     VDLELPEGRLIAIVGPNGAGKSTLLKAVLGLVPPVTGWVKIFGESYARRRSWVGYVPQ
                     RESVDWDFPTSALDVVTMGLYGQVGWFRRPGRHHRETALACLDKVGMRDLAHRQISQL
                     SGGQQQRVFLARALAQDARLYLMDEPFAGVDATTERAILTLLQELRASGRTVVAVHHD
                     LQTVAEYFDHVVLLNMRLVAAGPVATTFTPENLQRTYGGRLTALTQATEALLQKREAK
                     LETKLEGGKGEKPWS"
     gene            3518..4792
                     /locus_tag="VEL74_18385"
     CDS             3518..4792
                     /locus_tag="VEL74_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HYG64554.1"
                     /translation="MELSFTARNVLIGATLLGALGGLLGSFALLRRQSLLGDALAHAA
                     LPGVCLAYLLTGAKTPLPLFAGALAAGLLGSLLILAIVRWSRVKEDSAIGIILSVFFG
                     VGIVLLTHIQRLPSGNQSGLDKYLFGQAATLVVQDLQVMAVLGTVVLAAVLVFFKEFK
                     LISFDPGFAASLGVPVRRLEVLLTLLLVVVVVVGLQTVGVVLIVATLITPAAAARQWT
                     DRLGTMVALSAGIGALAGVAGSLASATVPRLPTGPVIVLVSSTVLIVSLLFAPGRGLL
                     WGILRERRLAARIRSENLLKDLYRMSEEAGDFAVYFTWPHLMGVRGQSAVELERSAGP
                     LLAQGLAERSGDALRLTEAGRREAGNVVRKHRLWEVYLTRRLELPVDHVHRDAEAMEH
                     ALTDAAVDNIEELLGFPGVDPHGRPIPARRTV"
     gene            4789..5910
                     /locus_tag="VEL74_18390"
     CDS             4789..5910
                     /locus_tag="VEL74_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HYG64555.1"
                     /translation="MSPTLVILLVASFVAASCALVGSFLVLRKMALLGDAISHAVLPG
                     IAIAFLLTGTRATLPMMLGAGALGVITVLLVELFNRSRRLREDASIGVVFPALFSLGV
                     ILISRYAAQVDLDLDCVLYGEIAYAPWDTLTIGGTDLGPKALWVTGGVLLVNAVWILL
                     CYKELKLSTFDAGLASALGFSPVLLHYILMSAVSVTVVGSFESVGAILVVAMLVVPPA
                     TAYLLTERLGTMLALSVALGVTSALGGYGLARAWDASIAGAMATVAGLQFLAALLFSP
                     GHGLLARLLILQRMGTRLSGQLVLLHLQKDEDHVVPLALLERRFGWSPLKLRRVLARL
                     LREGLVEHQRDGLHLTDRGARVLETTGRIELAHRLPTPG"
     gene            5935..6726
                     /locus_tag="VEL74_18395"
     CDS             5935..6726
                     /locus_tag="VEL74_18395"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HYG64556.1"
                     /translation="MNRYVIAAIATLLLAGPAGAFDLSGLPEPLVTDRPDFTESPSVV
                     PSGHFQVEGGATFTRVEEEDADTFGEILVRIGLGKGLEARIGVPSYERLDAQAGTISG
                     YGDPSLGFKLRLTPDPPSEKTPGRPEAALILSTSIPEGDEELSDGAWQPEARVALSWG
                     LGARFSLSSNVGYAYPEDPGSGDRFDQFLASLSLGIALTDRLGAFVEGYGFSGETVDG
                     SETTYMDAGLTWLVANDLQLDVRAGRGFNDADPDWFVGAGAALRW"
     gene            complement(6744..>7023)
                     /locus_tag="VEL74_18400"
     CDS             complement(6744..>7023)
                     /locus_tag="VEL74_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYG64557.1"
                     /translation="FYHAGDTALFSDMQLLGEIYRPELAFLPIGDLFTMDPDQAARAC
                     RYLGVRKVIPIHWGTFPALTGTPQKLVKCLESGGINCEVISLKPGETY"
BASE COUNT          961 a         2505 c         2461 g         1096 t
ORIGIN      
        1 ctccggcaac gccaacccgg gaggcccgca cctccacttc gccatcttcc agctcgggcc
       61 cgacaagaag tggcacgagg ggacgccgat caacccctac ccgctgatgg gcgggcgggg
      121 gggcgaccgc caggcgcggt gaccgacggc gcgcctcgcc actccggcgt ccgagacacc
      181 tcttggactt gacatacgag ggcaggagtg acagatttct gtcatgcaga cgacgttgag
      241 attggatgac gagctctatc ggcgagccaa ggctcaagcg gcggctcagg gacgcagctt
      301 gacgacgttt gtggaggagg cgatccggga acatctcaac aggccggcac gggtcgctcg
      361 gcagcggcca ctgcgcctgc cggtgtcgac ggcgacgggc ggattggccc ccggcttctc
      421 cagtctcgag gaggctgtag acgccgccga tctggctgca gaccggcacc tggccggctg
      481 acccatggat ctgctcgacg ccaacatcct catcggcgct ttccggcgtg atgacccgga
      541 ccatggggcg gtgaagggtt ggctggaaga gtcgctgagc cagggctctc tcatcacctt
      601 ccctcccctg gtcgaggtcg ccttccttcg gatcgtgacc caccctcgta tctttgcgac
      661 cccgtctccc ttcgaagaag ccatggagtt ccttcagatc cttcaggaga gcggccagct
      721 tcgcgagacg ccgtggaccg tacggatgag ggccgtgtgg tggcggtggt gtcgcgacct
      781 cgagttggtg ggtaacgacg tcaacgacgc ctaccttgcg gccaccgcta cggaagcgcg
      841 ctgccgcctg gtcaccaggg acggcggctt cagccgcttc cctggcctcg actggtggaa
      901 cccgatcggg ctccccgaaa aatgaccctc ccctagctac gccggctcga cgtggatctt
      961 cgacgccgcg cggaagccca gggtcagggg ctcgccggat tccaggcgca gctcgacggc
     1021 gtccgccgcc tcgtcgcggg agaggacgcg cagggcgttc cccggccgca ggccgcggcg
     1081 ctcgaccagt tgcaggaagt cgcggctctg gtcccccacc cgcgccaccc ggagcggcgc
     1141 ctccagaccg caggtcagca ggctcggcag gtccgggacc tccatctcgc cgtggatgtt
     1201 ggggatcggg tcgccgtgcg ggtcgaccga cgggtagccc agcatctcgt ccatccgctc
     1261 gatcagccgg tccgagacgg cgtgctccag gcgctcggcc tcggcgtgca cctcggtcca
     1321 gtccatcccc atcacctgca ccaggaagag ctcgatcagc cggtggcgcc gcagcacgtg
     1381 catcgcgagc tggttgccgg acggcgtcag ccgcacgccg ctgtagggct cgtaggtgac
     1441 caggccgctg tcggccagcg tcttcatcat cgccgtcacc gtgcccggcg cgagctgcag
     1501 ggcggacgag atccgccgca tcgagaccag agagtccggc tcccgctgct cctcgagcag
     1561 gatgcatttc aggtagtcct cgaccgtgct cgtcggcatc ggccagaggt taccgcagaa
     1621 atccttcgaa gtcctccctt gacattttgt ctagtcgtgg agcagaattt tggtatgcca
     1681 aaaatcctcc gactcctccc gctcctcctt ctcctcccgg cgctgctggt cctggcggcc
     1741 tgcgacggtc agggcggcgc ggcgtccagc gacggtgacg gcgaccggaa gctgcgcgtg
     1801 gtcgccacca ccggcatcat cgccgacacc gccgcccggg tggccggatc gcatgccgag
     1861 gtcgaggggc tcatgggccc cggggtcgat ccccacctct acaaggccag tgagagcgac
     1921 gtccgccggc tcgccgaggc cgacctggtg ctctacaacg gcctccacct cgaggggaag
     1981 atgggggaca tcctcggcaa gatggggcgc aaccggccgg tggtggcggt ggccgaggac
     2041 atcccgaaag agagcctgcg caccccgccc gagttccagg ggcagcccga cccccacgtc
     2101 tggttcgacg tctccctgtg ggcgaagacg ctggggccga tcgcgcgggc gctgtcggag
     2161 ctcgacctcg ggaacgcggc cgactaccag gccaacgccg ccgcttatga gaaggagctg
     2221 ctggagctgg acgcctgggt ggcggaggag gtcgcccgca tccccgaagg gcagcgggtg
     2281 ctggtgacgg cgcacgacgc cttcggctac ttcggccggc gctacggcgt cgacgtcatc
     2341 ggtctccagg ggatcagcac cctgtccgag gccgggctca acgacgtcga ccgggtggtc
     2401 cagatcgtgc tggaccggcg ggtgaaggcg attttcgtgg agtccagcgt gccgcgccgc
     2461 tccatcgagg cggtgcaggc ggcggcggcc tcccgcgggc accaggtgag gatcggcggc
     2521 cagctcttct cggactcgct gggcggcccg gcgacgccgg ccgccaccta ccccggcatg
     2581 gtgcgcgtca acgtcgagac catcgtcaag gccctgcgct aggagaaggc caggaaaagg
     2641 agtatcgcgg catgagcagc gcccccgtcc tcgagtcccg gttcccgccc accggcccgg
     2701 cagcggagcc ggccgccggc gtgccggccg tcgaggtgca cgacctcacc gtggcctacc
     2761 gcacccagcc ggtgctctgg gacgtcgacc tcgagctccc cgaggggcgg ctcatcgcca
     2821 tcgtggggcc gaacggcgcc ggcaagagca ccctgctcaa ggcggtgctc ggcctggtgc
     2881 ccccggtgac cggctgggtg aagatcttcg gcgaatcgta cgcccgccgc cgctcctggg
     2941 tcggctacgt gccgcagcgc gagagcgtcg actgggactt ccccaccagc gccctcgacg
     3001 tggtgaccat ggggctctac ggccaggtcg gctggttccg gcgccccggc cggcaccacc
     3061 gggagaccgc cctcgcctgc ctcgacaagg tggggatgcg cgacctcgcc caccgccaga
     3121 tcagccagct ctccggtggc cagcagcagc gcgtcttcct cgcccgggcg ctcgcccagg
     3181 acgcccgcct ctatctcatg gacgagccct tcgccggcgt cgacgccacc accgagcggg
     3241 cgatcctcac cctgctgcag gagctgcgcg ccagcggccg gacggtggtg gccgtgcatc
     3301 acgacctgca gaccgtggcc gagtacttcg accacgtggt gctgctcaac atgcgtctgg
     3361 tggcggccgg gccggtggcg accacgttca cgccggagaa cctgcagcgc acctacggcg
     3421 gccggctgac cgcgctcacc caggccaccg aggcgctgct ccagaagcgg gaggccaagc
     3481 tggagaccaa gctggagggc ggcaaggggg agaagccgtg gagctgagct tcaccgcccg
     3541 caacgtgctg atcggcgcca ccctgctggg ggcgctcggc gggctcctcg gcagcttcgc
     3601 gctgctgcgg cgccagagcc tgctcggcga cgccctggcc cacgcggcgc tgcccggggt
     3661 ctgcctcgcc tacctgctca ccggcgccaa gacgccgctc cccctgttcg ccggcgcgct
     3721 cgccgccggc ctcctggggt cgctgctgat cctcgccatc gtgcgctgga gccgcgtcaa
     3781 ggaggactcg gcgatcggca tcatcctgtc ggtcttcttc ggcgtcggca tcgtcctcct
     3841 cacccacatc cagcggctcc cctcgggcaa ccagagcggc ctcgacaagt acctgttcgg
     3901 ccaggcggcg acgctggtgg tccaggacct ccaggtgatg gcggtgctgg ggacggtggt
     3961 gctggccgcg gtgctcgtct tcttcaagga attcaagctg atctccttcg accccggctt
     4021 cgccgccagc ctcggagtgc ccgtgcggcg cctggaggtg ctgctgaccc tgctcctggt
     4081 ggtggtggtc gtcgtcggcc tccagaccgt gggcgtggtg ctgatcgtag cgacgctgat
     4141 caccccggcc gcggcggcgc gccagtggac cgaccggctg ggcaccatgg tcgccctgtc
     4201 ggcggggatc ggcgcgctgg ccggcgtcgc cgggtcgctg gcgagcgcca cggtgccgcg
     4261 cctgcccacc ggtccggtga tcgtcctggt ctcgagcacg gtcctcatcg tgtcgctgct
     4321 cttcgcgccg ggccggggcc tcctctgggg gatcctgcgc gagcgccggc tggccgcccg
     4381 catccgtagc gagaacctgc tcaaggacct ctaccggatg agcgaggagg cgggggactt
     4441 cgccgtctac ttcacctggc cgcatctgat gggggtgcgc ggacagagcg ccgtggagct
     4501 cgagcggagc gccgggccgc tcctcgccca ggggctcgcc gagcgctccg gcgacgccct
     4561 gcggctcacc gaggccggtc gccgcgaggc cggcaacgtc gtccgcaagc accgcctctg
     4621 ggaggtctac ctgacccgcc ggctcgagct gccggtcgac cacgtgcacc gcgacgccga
     4681 ggccatggag cacgccctga ctgacgccgc cgtcgacaac atcgaggagc tcctcggctt
     4741 ccccggcgtc gacccccacg gccggcccat cccggcgcgg aggacggtat gagcccgacg
     4801 ctggtgatcc tgctggtggc gagcttcgtg gcggcgagct gcgccctggt cggctcgttc
     4861 ctggtgctcc gcaagatggc gctcctcggc gacgccatca gccacgcggt gctccccggc
     4921 atcgccatcg ccttcctgct gaccggcacc cgggcgaccc tgcccatgat gctgggggcg
     4981 ggcgccctgg gggtgatcac ggtgctgctg gtcgagctgt tcaaccgcag ccgccgcctg
     5041 cgcgaggacg cctcgatcgg cgtcgtcttc cccgccctct tctccctcgg tgtcatcctg
     5101 atcagccgct acgccgcaca ggtggacctc gacctcgatt gcgtgctcta cggcgagatc
     5161 gcgtacgctc cctgggacac gctgacgatc ggcgggaccg acctcgggcc caaggccctc
     5221 tgggtgaccg gcggtgtgct gctggtgaac gcggtgtgga tcctgctctg ctacaaggag
     5281 ctcaagctct ccaccttcga cgccgggctc gcgtccgcgc tggggttctc gcccgtcctc
     5341 ctccactaca tcctgatgag cgcggtctcg gtgacggtgg tgggctcgtt cgagtcggtg
     5401 ggggcgatcc tggtggtggc gatgctggtg gtgccgccgg ccacggccta cctgctgacc
     5461 gagcggctgg ggacgatgct cgcgctgtcg gtcgccctgg gggtgacctc ggcgctgggc
     5521 ggctacggct tggcccgcgc ctgggacgcc tcgatcgccg gcgccatggc gacggtagcc
     5581 gggctgcagt tcctcgccgc cctgctgttc tcgccgggcc atggcctgct cgcccggctg
     5641 ctgatcctgc aacggatggg cacgcgcctc tcgggccagc tcgtgctgct gcacctccag
     5701 aaggacgagg accacgtggt gccgctggcg ctgctcgagc ggcgcttcgg ctggagcccg
     5761 ctgaagctcc ggcgggtgct ggcccgcctg ctgcgcgagg ggctggtcga gcaccagcgg
     5821 gacgggctgc acctgaccga ccgcggcgcc cgggtcctgg agaccaccgg ccggatcgag
     5881 ctggcccacc ggctgccgac gccgggatag agtgagagga ggagagggag attcatgaac
     5941 cgctacgtta tcgccgccat cgccaccctg ctcctggccg gaccggccgg agccttcgac
     6001 ctctcgggcc tccccgagcc cctggtcacc gaccggccgg acttcacgga gagcccgtcc
     6061 gtcgtgccct ccggtcactt ccaggtcgag gggggcgcca cgttcacccg cgtcgaagag
     6121 gaggacgccg acaccttcgg cgagatcctg gtgcgcatcg gcctgggcaa ggggctggag
     6181 gcgcggatcg gcgtcccctc ctatgagcgg ctggacgctc aggccggcac gatctccggc
     6241 tacggcgacc cctctctcgg cttcaagctc cgcctgacgc cggacccgcc ctcggagaag
     6301 acgccgggac gtccggaggc ggcgctgatc ctgagcacgt cgatccccga gggggacgag
     6361 gagctgagcg acggcgcctg gcagccggag gcgcgggtcg ccctctcctg ggggctgggc
     6421 gccaggttct cgctctcctc gaacgtcggc tatgcctacc ccgaggaccc ggggtccggc
     6481 gaccgcttcg atcaattcct cgccagcctg tcgctgggga tcgccctcac cgaccggctg
     6541 ggcgccttcg tcgaggggta cggcttctcc ggggagacgg tggacggctc cgagaccacc
     6601 tacatggacg cagggctcac ctggctggtg gccaacgacc tgcagctcga cgtgcgcgcc
     6661 gggcggggct tcaacgacgc cgaccccgac tggttcgtcg gtgccggcgc cgcgctgcgc
     6721 tggtaagcga cggctgagcg agcctagtag gtctcgccgg gcttgagaga gatgacctcg
     6781 cagttgatcc ccccgctctc caggcacttg accagcttct gcggcgtgcc ggtgagcgcg
     6841 gggaaggtcc cccagtggat ggggatcacc ttgcgtaccc cgaggtagcg gcaggcgcgg
     6901 gcggcctggt ccgggtccat ggtgaacagg tcgccgatgg gcaggaaggc cagctcggga
     6961 cggtagatct cccccaggag ttgcatgtcg gagaacaggg cggtgtcgcc ggcgtggtag
     7021 aag
//
LOCUS       DASPJI010000651         3551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_339395, whole genome shotgun sequence.
ACCESSION   DASPJI010000651 DASPJI010000000
VERSION     DASPJI010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339395"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VEL74_18405"
     CDS             complement(<1..1051)
                     /locus_tag="VEL74_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HYG64558.1"
                     /translation="MGDESVWRPSLGAVYLGENRSRFRVWAPLAETVELELICEGGRR
                     VSLEAEEGGYFAAETEDVPPGTRYLIHLDGKEGRPDPASRLQADGVHGPSVVVDPAFE
                     WGGEEERWRGLPLEDVILYELHVGTFTQEGTFDAVIPQLDRLRDLGVTALEIMPVAQF
                     PGERNWGYDGVLPFAVQNSYGGPEGLKRLVAACHARGLAVVLDVVYNHLGPEGNYLGQ
                     FGPYFTDRYKTPWGLALNFDGAHSDNVRWFFIHNALQWVDEFHFDGLRVDAVHAIVDH
                     SAEPFLQDLCDAVRNRARELGRRIYTIAESDLNDPRVISPKEEFGLGFDTQWADDFHH
                     SLHTLLTGEQDGYYSG"
     gene            complement(1150..3183)
                     /gene="glgB"
                     /locus_tag="VEL74_18410"
     CDS             complement(1150..3183)
                     /gene="glgB"
                     /locus_tag="VEL74_18410"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HYG64559.1"
                     /translation="MKDEPKSRVETLEAPAEAPVKTLEAPVETAAPSEEEVAAAPRGG
                     SPLTDHDLYLFNEGTHFRLHDKLGAHAGEKDGVRGVWFAVWAPDAATVSVVGDWNEWD
                     RHADRMSPRGGSGVWEGFVPGVEKGAHYKYHIESRFGGFRVDKADPFGVLHETPPLTA
                     SVVWDLEYEWGDDEWMASRGGRQSLAAPMSIYEMHLGSWRRVPEEGNRSLTYREMAPL
                     LAEHVRGLGFTHVELLPVMEHPFFGSWGYQVTGFFAPSSRYGSPQDLMYLIDYLHQQG
                     IGVILDWVPSHFPTDEHGLAYFDGTHLFEHADPRQGFHPDWGSFIFNYDRNEVRSFLT
                     SNAIYWLDKYHADGLRVDAVASMLYLDYSRKAGEWIPNRFGGRENLGAIHFLRTTNEE
                     VYETFPDAQTVAEESTAWPMVSRPLYVGGLGFGYKWDMGWMHDTLAYMAYDPVFRKYH
                     HNQMTFRGMYAWTENFVLPLSHDEVVHGKGSLLGKMPGDLWQKLANLRLLYGYMFAQP
                     GKKMLFMGAELAQIREWNHDSSLDWHLLGDERHAGISRWLGDLNRVYREEPALYELDC
                     NPDGFEWIDANDAENSTLSFLRKSRDSQDTVLAVFNFTPIPRQNYRVGVPGGGFWKEL
                     LNSDAPTYGGSGVGNFGGVEAMPVPLHGRPHSLTLTLPPLGVVFFRRETPAAS"
     gene            complement(3188..>3551)
                     /locus_tag="VEL74_18415"
     CDS             complement(3188..>3551)
                     /locus_tag="VEL74_18415"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HYG64560.1"
                     /translation="DLQELGFFDADQARAERERRGEIRRGMAEALPGDRRGDEETTYR
                     EVLRDRLRHLASSPARMVLVNLEDLWGETRPQNVPGTSNERPNWRRKARLSFEEFSSR
                     DDVVDLLREVDELRRRRE"
BASE COUNT          552 a         1233 c         1196 g          570 t
ORIGIN      
        1 agccggagta gtagccgtcc tgctcgccgg tgagcagcgt gtgcagggag tgatggaagt
       61 cgtccgccca ctgcgtgtcg aagccgaggc cgaactcctc cttcggggag atcacgcgcg
      121 ggtcgttgag gtcactctcg gcgatcgtgt agatgcggcg gccgagctcc cgggcgcggt
      181 tgcgcacggc gtcgcagagg tcctgcagga agggctccgc ggagtggtcg acgatggcgt
      241 gcaccgcgtc gacgcgcagg ccgtcgaagt gaaactcgtc gacccactgc agggcgttgt
      301 ggatgaagaa ccagcgtacg ttgtcgctgt gcgcgccgtc gaagttgagg gccagacccc
      361 agggggtctt gtagcggtcg gtgaagtacg gtccgaactg gccgaggtaa ttcccctcgg
      421 ggccgaggtg gttgtagacc acgtccagca ccaccgcgag gccgcgggcg tggcaggccg
      481 ccaccagccg cttcagccct tccgggccgc cataggaatt ctgcaccgcg aacggcagga
      541 cgccgtcgta gccccagttc cgttcgcccg ggaactgcgc caccggcatg atctcgagcg
      601 cggtcacccc gaggtcgcgc aggcggtcga gctgcgggat caccgcgtcg aaggtgccct
      661 cctgagtgaa ggtcccgacg tgcagctcat agaggatgac gtcctccagc ggcaggccgc
      721 gccaccgctc ctcctcccct ccccactcga aggcgggatc gacaacgacc gaggggccgt
      781 gcacgccgtc ggcctggagc cgcgaggcgg gatcgggacg cccctctttc ccgtccaggt
      841 gaatcagata gcgcgtgccg ggaggcacgt cctcggtctc cgccgcgaag tagccgccct
      901 cctccgcttc cagggagacg cggcgcccgc cctcacagat cagctccagc tccaccgtct
      961 cggccagcgg cgcccagacc cgaaagcggc tacggttctc ccccagatac acggcgccca
     1021 ggctcggccg ccagacggac tcgtctccca tgctcatccc tttcaaactg caggatcatc
     1081 gactgcaaga aaccccgaag ccgtggaccg gctcgggggt tttcgtatgg aagcaagaag
     1141 gaggccagat caggaggctg ccggcgtctc gcgccggaag aacaccacgc ccagaggtgg
     1201 cagggtgagc gtcagcgagt gcggccggcc gtggagcggt accggcatgg cctcgacgcc
     1261 tccgaagttg cccacgccgc tgccgccgta ggtcggtgcg tcgctgttga gcagctcctt
     1321 ccagaatccc cctcccggca cccccacccg gtagttctgg cgcgggatcg gcgtgaaatt
     1381 gaagaccgcg agaactgtgt cctgactatc ccggctcttg cggaggaagg agagagtgct
     1441 gttctcggcg tcgttggcgt cgatccactc gaagccgtcc gggttgcagt cgagctcgta
     1501 cagcgccggc tcctcgcggt aaacgcggtt gaggtcgccc agccagcggg agatcccggc
     1561 gtgccgctcg tcacccagga ggtgccagtc gaggctcgag tcgtggttcc actcccggat
     1621 ttgcgccagc tccgccccca tgaacagcat cttcttgccc ggctgggcga acatgtagcc
     1681 atagagcagc cgcaggttcg ccagcttctg ccagaggtcg ccgggcatct tccccagcag
     1741 cgagcccttg ccgtgcacca cctcgtcgtg cgagagcggc agcacgaagt tctcggtcca
     1801 ggcgtacatc cccctgaacg tcatctggtt gtggtggtac ttgcggaaca ccgggtcgta
     1861 cgccatgtag gccagggtgt cgtgcatcca gcccatgtcc cacttgtagc cgaagccgag
     1921 gccgccgacg tagagcgggc gcgacaccat cggccaggcg gtggactcct cggccacggt
     1981 ctgggcgtcg gggaaggtct cgtagacctc ctcgttggtg gtgcgcagga agtggatggc
     2041 gccgaggttc tcccggccgc cgaagcggtt ggggatccac tcgcccgcct tgcgcgagta
     2101 gtcgaggtag agcatggagg cgacggcgtc cacccgcagg ccgtcggcgt ggtacttgtc
     2161 cagccagtag atggcgttgc tcgtgaggaa gctccgcacc tcgttgcggt cgtagttgaa
     2221 gatgaagctc ccccagtcgg ggtggaagcc ctgccgcggg tcggcgtgct cgaagaggtg
     2281 ggtgccgtcg aagtacgcca ggccgtgctc gtcggtcggg aagtgcgagg gcacccagtc
     2341 gaggatgacg ccgatcccct gctggtgcag gtagtcgatc aggtacatca ggtcctgggg
     2401 cgagccgtag cggctggagg gggcgaagaa gccggtgacc tgataccccc aggagccgaa
     2461 gaacgggtgc tccatcaccg gcaggagctc cacgtgggtg aacccgaggc cgcggacatg
     2521 ctcggcgaga agcggcgcca tctcgcggta ggtgagggag cggttcccct cctcgggcac
     2581 acggcgccac gagcccaggt gcatctcgta gatggacatg ggcgccgcca gcgactgccg
     2641 cccgccacgg ctcgccatcc actcgtcgtc cccccactcg tactccaggt cccagaccac
     2701 cgaggcggtg agcggcggcg tctcgtgcag aaccccgaac gggtccgcct tgtccacccg
     2761 gaagccgccg aagcgggact cgatgtggta cttgtagtgg gcccccttct ccactccggg
     2821 cacgaagccc tcccagacgc ccgagccgcc gcgcggcgac atccggtcgg catggcggtc
     2881 ccattcgttc cagtcgccga ccacggagac cgttgccgcg tccggcgccc agaccgcgaa
     2941 ccagacgccg cggacgccgt ccttctcgcc ggcgtgggcc cccagcttgt cgtgcagccg
     3001 gaagtgcgtg ccctcgttga aaaggtagag atcgtgatcg gtgagcgggc tgccgccccg
     3061 gggcgccgcc gccacctcct cttcggacgg cgccgccgtc tccaccggcg cctccaacgt
     3121 cttgacaggc gcctccgccg gcgcctccag cgtctccact ctgctcttcg gctcgtcctt
     3181 catacggcta ctcccttctc cgcctcagct cgtccacctc gcgcagcagg tccacgacgt
     3241 cgtcgcggct cgagaactcc tcgaacgaga ggcgcgcctt gcggcgccag ttgggccgct
     3301 cgttgctcgt gcccggaacg ttctgcggcc gagtctcgcc ccacaaatcc tcgaggttga
     3361 ccagcaccat gcgcgccggg ctcgacgcca ggtgccgcag gcggtcgcgc agcacctcgc
     3421 ggtaggtggt ctcctcgtcg ccgcgacggt ccccggggag cgcctcggcc atgccccgcc
     3481 ggatctctcc ccggcgctcg cgctcggcgc gcgcttggtc ggcgtcgaag aacccaagct
     3541 cctggaggtc g
//
LOCUS       DASPJI010000652         9143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_555342, whole genome shotgun sequence.
ACCESSION   DASPJI010000652 DASPJI010000000
VERSION     DASPJI010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9143)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9143
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555342"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>9143
                     /locus_tag="VEL74_18420"
     CDS             <1..>9143
                     /locus_tag="VEL74_18420"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64561.1"
                     /translation="RLEFMLADSSASVLLTGSGGPGADLGRGTGLVRLDPRSVASPDS
                     DVALTAPARVSSRNLAYLIYTSGSTGRPKGVAVEHRSAVALVRWARQVFTPEELAGVL
                     ASTSVCFDLSVFELFVPLACGGKVILARNALELPALPAASEVTLVNTVPSAMAELARG
                     GRLPASLRSLRTVNLAGEPLQRALIDRLYELPGISRVYNLYGPSEDTTYSTWELVGRD
                     GGAPLIGRPVAGTRAYLLDARAPWAMAPVPAGVPGELLLAGEGLARGYLDQPGLTAER
                     FVPDPFARLFGGEPGSRLYRTGDLARWRADGRLEFLGRIDHQVKVRGFRIELGEIEAV
                     LALHPGLQDVAVTVRRDPEVGERLIAYVVPASVEAAVEPGAEPPASLAASLRSWLART
                     LPDYMIPAAWVTLPALPLTPNGKVDRRALPAPGTGEAAPRTPPRTPVEERLAAIWSEV
                     LGGVPVGVFDSFFELGGHSLLATRVLARVAEAFGVELPLAALFQAPTVAALAESLAGL
                     PATAQRLAAAPPEDGAPLSLAQQRLWFIDRLEPGAPAYNLWVAVRFGGDLRVGHLGRA
                     LTEVLRRHEPLRTVFAATDRGPEQVILPPSSPGELPWTLIELSGLPPARRQKARREAL
                     RVEALRPFDLAHGPVVRFLLVRLGDGEHVLMVTFHHIAADGWSLEVFFRELSALYAAS
                     AAGRPSPLPELPLRYADFAAAQRRSLTGGALAGHLAYWRQELAGLPVLELPLDRPRPP
                     ARSYRGASLPLDLPASLVDGLHRLAHAEGATLFMALLTGFATLLARTAGQGDFAVGVP
                     TAGRERVETEGMVGLFINTLALRVRMADDPPFGEMVRQVRQTVLAGQTHEVPFEKLVE
                     ELRPERSGTISPLFQVLFAFLGAARRERVEMPGLTLELQEVETAAAKFDLTLSMHERD
                     GRVHGWLDYPTDLFEASTATRWVGHLLTLLQGAAEYPDMSVGDLPMLSPEETGQILLR
                     WQGPRVELPGAKLVHRLVTEQASRTPERVAVESEAGRWTYAELEERASSLASRLRALG
                     IGPESRIGVCLDRSLELMAALLAIWKAGGAYVPLDPFYPHERLRLILDDARPSLLLTG
                     PGAPPDLAPPGLSVLPLGSLAEAGSARSSERLPAGDANQLAYIIYTSGSTGQPKGVMI
                     SHGAIVNRLLWMKAEFPLDERDAVLQKTPLSFDPSLVELFVPLMSGARVVMARPGGHL
                     DPAYMARAVAEHRISVLQLVPSVLSAFLEEDGIAGCQGLRHLLSGGEVLTPALRDRAL
                     DRVGAELCNFYGPTEYAVDMAFWRCRRGDPLAIVPIGRPIANTRAYVLDSRLRPVPAG
                     TVGELYVAGAGLARGYVGRPDLTAERFVPDPLSGVPGGRLYRTGDLVRQLPPGRGADG
                     ALEYMGRADLQVKVRGVRIEPGEVESWLVRHPVVAEAVVGVRGELLVAWLVPRGTSSA
                     SSAELRSFLAERLPDAMVPSAFVILPELPRMGSGKVDRAALPAPTTETWQTGSGVPRT
                     DIEELLSGLWAEVLGREQVGTDESFFDLGGHSLLATRLISRMRSALGVEISLGRLFEE
                     PTVARLARWAEAALRQEDTAEAEPEPPLEPQPHAGPQPLSFAQERLWFLDRLDPGPAY
                     NVPAAARLTGRLDPAALATALDEVVRRHEVLRTRFGQEGNRPVQVVEPWGPLRAARLP
                     LVDLSLLPGRARDTEAARLAAGEAARPFDLGSGRLVRSTLLRLAAAEHVLLLTLHHIV
                     ADGWSMDVLLSDVSAFYGSAVTGDALLLPDLPIQYVDFALWQRRHMSGYRLETQLEHW
                     RSRLAGAPEVFDLPADHPRPLGGGSRGGRLPLRLPAGLAAALTALGRHEGWTPFMGLL
                     AAFQALLARYSSQLDVVVGSPIANRNRLETEGLIGFFVNTLALRLDLTGDPPFAELAR
                     RARAVALDAYAHQDLPFEKLVEELAPQRLPGLTPLFQTLLVLQNAPLRLAMPGLAVEP
                     LEVDNGTAKFDLVLVLMEQAGELAGGAEYRRDLFEPATIARFVDHFAALLSSAVGDPG
                     RRLGDLELLGEGERQQLMVEWSDAAWPAASALPTVLSLFEQRAEEAPEATALVVRGER
                     LSYGELDRRAGRLARRLRRLGIGRSGRGRVGLLLARSAEMVESILAVWKAGAAYVPLA
                     PELPAERLAWMAENAGIAAVVAWEQAPEWGVPALLLAEEVSAGESGKATSGMAEAGDL
                     AYLIYTSGTTGRPKGVMVEHRSLSHTLLGMAWRFGFNAADRMPHLASFSFDISLFELF
                     APLIAGGTVELLTRDEVLDLPLLTACLRQATRWHAVPSLMRRVVDSLADTEGIAGGVG
                     GLRTVFVGGDRVPPELLRDMRRVFPDSELVVLYGPTEAAIVCSAYAVPAVLPVSPSVG
                     GGELERSLIGGPLPGVHLLVLDRWGSPVPIGVLGEIHIGGPGVSRGYHGWPGLTAARY
                     VAAPDEPGRRLYRTGDLARHLPDGTLEFVGRVDQQVKIRGFRVEPGEIEAALRSCPEV
                     AEAVVVAAPDRRGESRLAAYLVPARAEVLDERWARERMVGELREALSGRLPDYMIPVA
                     WVVLPSLPLSPNGKVDHGQLPEPDDAEGTATHVPPRSPAEDLLAGIWCEVLGLERVGV
                     QTSFFDLGGHSLLATQVMSRVREAFGVDLPLRRLFEAPTVAELARTVEAQLAGGGGAA
                     ETITRASRDQPLPLSFSQERLWVLDRLQSGDTAYNLPLTLRVDGQLDVPVLAAAFGEI
                     IRRHETLRTTFTERDGEPVQVIHPAIPPDGVLHLPVVDLHGLAAYFREPEAGRLVASE
                     SARSFDLSRGPLLRTLLLRAGAGEHLLLVHMHHIVSDAWSMGVLVHEVEVLYNSFRAG
                     RSSSLPELPVQYADFAVWQRGYLTGATLERQLGYWRERLTGAPGLDLPTDQPRPAVRS
                     GHGAEHRFVVDAATSAALLALARRQGVTPFMLLAAALLTLLARFSGQPDLSIGSPIAN
                     RTRIETEELIGFFVNTLVLRAELGRSTDFLDLLRQVREITLGAYAHQDIPFEELVAAL
                     HPDRDLSHTPLFQVMFALQNATVRTVRLEGIEISPLEVPSGTAKF"
BASE COUNT         1081 a         3242 c         3336 g         1484 t
ORIGIN      
        1 gcggctggaa ttcatgctgg ccgactccag cgcctccgtc ctgttgaccg ggagcggcgg
       61 gccgggagcg gacctgggcc ggggcaccgg cttggtgcgg ctcgatcctc gctcggtcgc
      121 ctcgccggac agcgacgtgg ccctgacggc gccggctcgc gtctcgtcgc ggaacctggc
      181 ctacctcatc tatacctcgg gctccacggg gaggcccaag ggggtggcgg tggagcaccg
      241 gagcgccgtg gcgctcgtgc gctgggcgcg gcaggtcttc acccccgagg agctggcagg
      301 cgtcctggct tcgacatcgg tctgcttcga tctgtcggtc ttcgagctgt tcgtgccact
      361 cgcctgcggc ggcaaggtga tcctggcgcg caacgccctg gagctgcccg cattgcccgc
      421 ggcctccgag gtcaccctgg tcaacacggt tccctcggcg atggcagagc tggcgcgcgg
      481 cggccggctc cccgcctccc tgcgatcctt gcggacggtg aacctcgccg gcgagccgtt
      541 gcagcgggcc ctgatcgacc gcctctacga gttgccgggc atctccaggg tctacaacct
      601 ctacggcccg tcggaggaca ccacctactc cacctgggag ctggtggggc gggacggcgg
      661 cgcgccgctc atcggccggc cggtcgccgg cacccgcgcc tacctgctgg acgcccgcgc
      721 gccgtgggcg atggcgccgg tgccggccgg cgttcccggg gagctgctgc tggcgggcga
      781 ggggctggcg cgcggctatc tggatcagcc cgggctgacg gcggagcgct tcgtgcccga
      841 ccccttcgcg cgcctgtttg gaggggagcc ggggagccgg ctctaccgca ccggggacct
      901 cgcccgctgg cgcgcggacg ggcggctcga gttcctcggg aggattgacc accaggtcaa
      961 ggtgcgcggc ttccgtatcg agctggggga gatcgaggcg gtcctcgcgc tccaccccgg
     1021 cctccaggac gtggcggtga cggtccgccg tgatcccgag gtgggggagc ggctgatcgc
     1081 ctacgtggtg ccggcttcag tggaggccgc ggtggagccg ggggccgagc cgccggccag
     1141 cctggcggcg tctctccgct cctggctcgc ccgcaccctg cccgactaca tgattcccgc
     1201 ggcctgggtg accctgccgg cgctgcccct cactcccaac ggcaaggtcg accgccgggc
     1261 gctgccggcg cccgggaccg gagaggccgc gcctcgcacc ccgccccgca ctccggtcga
     1321 ggagcggctg gcggcgatct ggagcgaggt tctggggggc gtcccggtcg gcgtcttcga
     1381 cagcttcttc gagttgggcg ggcactccct gctggcgacg cgcgtgctcg cccgcgtggc
     1441 cgaggccttc ggagtcgagc tgccgctcgc cgcgctgttc caggcgccca cggtggcggc
     1501 gctggcggag tcgctggccg gtctccccgc caccgcgcaa cgcttggcgg cggcaccgcc
     1561 ggaggatggc gcccctctct ccttggccca gcagcgcctc tggttcatcg accgcctgga
     1621 gcctggggcc ccggcttaca acctgtgggt ggcagtgcgg ttcggcggag acctccgggt
     1681 cggccacctc gggcgggcgc tcaccgaggt cctgcgccgc cacgagcctc tgcggaccgt
     1741 cttcgccgcc accgaccgtg ggccggagca ggtgatcctg ccgccgtcat ctccaggcga
     1801 gctgccgtgg accctgatcg agctctccgg gctgccgccg gcgcggagac agaaggcccg
     1861 gcgtgaggcg ttgcgggtcg aggcgttgcg gccgttcgac ctggctcacg ggccggtggt
     1921 ccgcttcctc ctggtgcggc tcggggacgg cgagcacgtg ctgatggtca ccttccatca
     1981 catcgccgcg gatggctggt cgctggaggt cttcttccgc gagctgtcgg ccctctatgc
     2041 cgcctctgcc gccgggcggc cgtcgccgct gccggagctg ccgttgcgct atgcggactt
     2101 cgccgcggcg cagcggcggt cgttgaccgg cggcgcgctg gcaggtcatc tggcctactg
     2161 gcggcaggag cttgccgggc tgccggtgct cgagctgcct ctggaccgcc cccgtccgcc
     2221 agcgcggagc taccggggag cgagcctgcc gctcgatctg cccgcgagtc tggtggacgg
     2281 gctgcaccgg ctggcccacg ccgagggggc cacgctgttc atggcgctgc tgaccggttt
     2341 cgccactctg ctcgcccgca cggcggggca gggggacttc gcggtcggcg tgcccaccgc
     2401 tggccgggag cgggtcgaga ccgaggggat ggtcggcctg ttcatcaaca ccctggcgct
     2461 gcgggtgcgg atggcggacg atcctccgtt cggcgaaatg gtgcggcagg ttcggcagac
     2521 tgtcctcgcc ggccagacgc acgaggtgcc gttcgagaag ctggtggaag agctccgccc
     2581 ggagcgctcc ggcaccatct cgccgctctt ccaggtgctc ttcgcattcc tgggcgccgc
     2641 caggcgggaa cgggtggaga tgcccgggct gaccctcgag ctccaggagg tggagaccgc
     2701 cgcggccaag ttcgacctga cgctgtcgat gcacgagcgg gacggccgcg tacacggctg
     2761 gctggactat ccaaccgatc tcttcgaggc gtccaccgcc actcgctggg ttggccatct
     2821 cctgaccctc ctccagggag cggccgagta tccggacatg tctgtcggcg acctcccgat
     2881 gctctcgccg gaggagaccg gccagatcct cctgcgctgg caggggccgc gggtggagct
     2941 ccccggcgcg aagctcgtgc accggctcgt gaccgagcag gcgtcccgga caccggagcg
     3001 cgtggcggta gagtccgaag ccggccggtg gacctatgcc gagctggaag agcgggcctc
     3061 ctctctggct tcccgcctta gggccctggg gattggcccg gagagccgca tcggcgtctg
     3121 cctggaccgt tcgctcgagc tgatggcagc cctcctggcg atctggaagg ccggcggcgc
     3181 ctatgtcccc ctggacccct tctatcccca tgagcgcctg cgcctgatcc tcgacgacgc
     3241 ccgtccgtcg ctcctcctca ccggccccgg agctccgccc gatctggccc cgcccggcct
     3301 ctccgttctg cctctgggct ccctggcgga agcgggcagc gcccgttcaa gcgagagatt
     3361 gcctgccgga gacgctaacc agctcgccta catcatctac acctccggct ccaccggtca
     3421 gccgaagggg gtgatgatct cccacggcgc catcgtcaac cggctgctct ggatgaaggc
     3481 cgaattcccg ctggatgaga gggatgcggt cctccagaag acccctttga gcttcgaccc
     3541 ctccctggtc gagctgttcg tgccgctgat gagcggggcc cgtgtggtga tggcaaggcc
     3601 gggcgggcac ctcgacccgg cctacatggc ccgcgccgtg gccgagcacc gcatctccgt
     3661 gctgcagctc gtgccctcgg tgctgagcgc cttcctggag gaggacggga tcgcgggctg
     3721 ccaggggctg cgccacctgt tgagcggcgg cgaggtgctc acgccggcgc tccgggaccg
     3781 tgccctggac cgcgtgggag ccgagctgtg caacttctac ggtcccaccg agtacgccgt
     3841 tgatatggct ttctggcgct gccgccgcgg cgaccccctg gccatcgtgc ccatcggacg
     3901 gcccatcgcc aacacccggg cctacgtgct ggacagccgt ctgcggccgg tgcccgccgg
     3961 cacggtgggg gagctgtacg tggccggagc gggcctcgcc cgtggttatg tgggccgtcc
     4021 ggacctgacg gcggagcggt tcgtgcccga tcctctgtcc ggcgtgccgg gtggccggct
     4081 ctaccggacc ggcgacctgg tgcggcagtt gcctcccggt cggggggctg acggcgccct
     4141 ggagtacatg ggccgtgccg acctccaggt gaaggtgcgg ggggtacgga tcgagccggg
     4201 cgaggtggag agctggctcg tgcgtcaccc cgtggtggcc gaggcggtgg tgggggtacg
     4261 gggcgagctc ctggtggcct ggctggtgcc ccgcggtacc tcctcggcct cttccgccga
     4321 gctgcggtca ttcctggcgg agcgcctgcc cgacgccatg gtgccttcgg cctttgtgat
     4381 cctgccggag ttgccacgga tggggagcgg caaggtggac cgggctgcgc tgccggcacc
     4441 caccaccgaa acatggcaga ctggctccgg cgtcccccgt acggacatcg aggagctgct
     4501 ctccggactc tgggccgagg tgctggggcg cgagcaggtg gggaccgacg agagcttctt
     4561 cgatctcggc ggccactcgc tcctcgcgac ccggctcatc tcccggatgc ggtcggccct
     4621 gggcgtcgag atctccctgg gccgcctgtt cgaggagcct acggtggcga gactcgcgcg
     4681 gtgggccgaa gcggctctcc gccaggagga tacggcggag gcagagcccg agccgccgct
     4741 cgagccgcag ccgcacgcgg gaccgcagcc gctgtccttc gcgcaggaac ggctctggtt
     4801 cctcgaccgg ctcgatcccg gcccggcgta caacgtccct gccgccgcgc gtctgaccgg
     4861 acggctcgat ccggccgccc tggcgaccgc tctggacgag gtcgtccgcc gccatgaggt
     4921 cctgcggacg cgcttcggtc aggaggggaa ccggccggtg caggtggtcg agccctgggg
     4981 ccccctgcga gccgctcgcc tgcctcttgt cgacctctcg ctcctgcccg ggagagcgcg
     5041 tgacaccgag gcggcgcgcc tggctgccgg cgaggccgcc cgcccgttcg atctggggag
     5101 cggccggttg gtgcgctcca cgctcctgcg gctcgcggcc gcggagcacg tcctgctgct
     5161 gacgctgcac cacatcgtgg cggacggctg gtccatggac gtgctgctct ccgacgtctc
     5221 ggctttctac ggctcggccg tcaccggtga cgctttgctg ttgcccgacc tcccgatcca
     5281 gtacgtggac ttcgcgctct ggcagcggcg gcatatgtcg gggtaccgtc tggagaccca
     5341 gttggagcac tggcgcagcc ggctcgccgg cgcgccggag gtcttcgatc tacctgccga
     5401 ccacccccgc ccgctcggcg gcggctcgcg gggcgggcgc ctcccgttgc gcctgcccgc
     5461 cggcctcgcg gccgcgctga cggccctcgg acggcacgaa ggttggacgc cgttcatggg
     5521 cctcctggcc gcctttcagg cgctgctcgc ccgctacagc agccagctcg acgtggtggt
     5581 ggggtctccc atcgccaacc gcaaccgcct ggagaccgaa gggctgatcg gcttcttcgt
     5641 caacaccctg gcgttgcgcc tcgacttgac gggcgatcca ccgttcgccg agctggcccg
     5701 ccgggcccgc gccgtggcgc tcgacgccta cgcacaccag gatctcccgt ttgagaagct
     5761 ggtggaggag ctggcgccac agcggctgcc gggcctgacg ccgctgttcc agaccctgct
     5821 cgtgctccag aacgcgccgc tgcgcctcgc gatgccggga ctcgccgtcg aacccctgga
     5881 ggtggacaac ggcaccgcca agttcgatct cgtgctggtg ctgatggagc aggcgggaga
     5941 gctcgccgga ggggccgagt accgccgcga cctgttcgag cccgccacca tcgcgcgctt
     6001 cgtcgatcac ttcgccgcgc tgctctcctc ggcagtggga gacccgggcc gccggctcgg
     6061 cgatctcgag ctgctcggcg agggcgagcg ccagcaactg atggtggagt ggagcgatgc
     6121 cgcctggccc gcggcttcgg ccctgcctac ggtcttgtcg ctgttcgagc agcgggcgga
     6181 ggaggcgccg gaggccacgg cgttggtggt ccggggcgag cggctgagct atggcgagct
     6241 cgaccggcgc gccgggcggc tggcccgccg cctgcgccgt ctcggcatcg gcaggagtgg
     6301 acgcggccgg gtcggcctgc tgctggcgcg ctcggccgag atggtcgagt cgattctggc
     6361 ggtctggaag gccggcgccg cctacgtgcc cctggcgccc gagctgccgg cggagaggct
     6421 ggcctggatg gccgagaatg ccggtatcgc cgccgtcgtg gcctgggaac aggcaccgga
     6481 atggggtgtg cccgcacttc tgctggcgga agaagtctcc gccggcgaga gcgggaaggc
     6541 cacttccggt atggcggagg cgggggacct cgcctatctg atctacacct ccggcaccac
     6601 gggccgcccc aaaggggtga tggtggagca ccggagcctg tcgcacactc tgctgggaat
     6661 ggcttggcgg ttcggcttca acgctgcgga ccgcatgccg cacctggcct ccttctcctt
     6721 cgacatctcg ctcttcgagc tgttcgctcc gctcatcgcc ggagggacgg tggagctgct
     6781 cacccgcgac gaggtgctcg acctgccgct cctgaccgcg tgcctccggc aggcgacgcg
     6841 gtggcacgcc gtgccgagcc tgatgcggcg ggtggtggac tcgctggcag ataccgaagg
     6901 cattgctgga ggtgttgggg gtctgcggac ggtcttcgtc ggcggcgacc gggtgccgcc
     6961 ggagctgttg cgcgatatgc ggcgggtgtt ccccgactcg gagctggtgg tgctgtacgg
     7021 cccgaccgag gcggccatcg tctgctcggc gtacgccgtg cctgccgtgt tgccggtatc
     7081 gccgtcagta ggggggggag agctggagcg cagcctgatc ggcggtccac tgccgggggt
     7141 ccacctgctg gtgctggacc gctgggggag cccggtgcca ataggggtgc tgggagaaat
     7201 ccacatcggc ggacccggag tgagccgcgg ctatcacggc tggccgggtc tgacggcggc
     7261 gaggtacgtc gcggcacccg atgagccggg ccgccggctg taccggaccg gagacctggc
     7321 ccgccacctg ccggacggta ccctcgagtt cgtggggcgg gtggaccagc aggtcaaaat
     7381 ccgcggcttc cgggtggagc cgggggagat cgaggcggcg ttgcggtcct gccctgaggt
     7441 ggcggaggcg gtggtggtcg cggcaccgga taggcgtggc gagagccgct tggcggctta
     7501 tctggtgccg gcgagggccg aggtcctgga cgagcggtgg gcccgggagc gcatggtcgg
     7561 cgagttgcgg gaggcgctgt ccggccggct gccggactac atgatcccgg tcgcctgggt
     7621 cgtcctgccc tccctgcccc tctcgcccaa tggcaaggtg gaccatggcc agctgccgga
     7681 gccggatgac gcggagggga ccgccaccca cgtccccccc cgcagcccgg ccgaggatct
     7741 gctcgccggg atctggtgtg aggtactggg gctggagcgc gtcggcgtcc agaccagctt
     7801 cttcgacctg ggcggccact ccctgctggc cacccaggtc atgtcccgag tgcgcgaggc
     7861 cttcggtgtg gacctgccgt tgcgccggct gttcgaggcg cccaccgtgg cggagctggc
     7921 gcggacggtc gaggcgcagc tcgctggggg tggaggggcc gcggagacga tcacgcgggc
     7981 gtctcgagac cagccgctac ccctctcctt ctcccaggag aggctgtggg tcctcgaccg
     8041 cctgcagtcc ggcgacacgg cctataacct gccgctgacg ctgcgtgtgg acggccaact
     8101 cgatgtgccg gtcctcgccg ccgccttcgg cgagatcatc cggcgccacg agaccctgag
     8161 gaccactttc accgagcgcg atggtgagcc ggtgcaggtg atccacccgg ccatcccgcc
     8221 ggatggagtg ctgcacctgc cggtcgtcga tctgcacggg ctcgccgcgt acttccggga
     8281 accggaggcg gggcgccttg tcgcgagcga atccgcccgc agcttcgatc tctcccgggg
     8341 gccgctgctc cggacgctgc tgctgcgggc cggggccggc gagcacctgc tgctggtgca
     8401 catgcaccac atcgtctcgg atgcctggtc tatgggtgtc ctcgtgcacg aggtggaggt
     8461 cctctataac tccttccgcg ccggccgctc ctcatccctg ccggagctgc cggtccagta
     8521 tgcggacttc gccgtctggc agcgcggcta tctcaccggt gcgacactgg agcggcagct
     8581 cggctactgg cgcgagcggc tgaccggcgc tcccggtctc gacctgccca ccgaccagcc
     8641 acgaccggcg gtgcggtcgg gtcatggggc cgagcaccgc ttcgtggtcg acgccgccac
     8701 ctccgcggcc ctgctcgccc tcgcccgccg ccagggggtg accccgttca tgctgctggc
     8761 ggcggcgctg ctcaccctgc tcgcgcgctt cagcggacag cccgatctat ccataggctc
     8821 gccgatcgcc aatcgcaccc ggatcgagac ggaggagctg atcggcttct tcgtcaatac
     8881 cctggtgctg cgcgccgagc tcgggcggtc caccgatttc ctggatttgc tgcgccaggt
     8941 gcgggagatc accctcggcg cctacgccca ccaggacatc ccattcgagg agctggtggc
     9001 ggcgctccat ccggaccgcg acctgtcgca cacgccgctc ttccaggtga tgttcgcgct
     9061 gcagaacgcc actgtccgca cggtccggct cgaggggatc gagatctccc ccctggaagt
     9121 cccctccggc accgccaagt tcg
//
LOCUS       DASPJI010000653         4153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_555356, whole genome shotgun sequence.
ACCESSION   DASPJI010000653 DASPJI010000000
VERSION     DASPJI010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555356"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1853)
                     /locus_tag="VEL74_18425"
     CDS             complement(<1..1853)
                     /locus_tag="VEL74_18425"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG64562.1"
                     /translation="MSVASRISSLSPEQRALFELLKKKSKAAAPKVHQPPPVVRHSGP
                     TGEGDWPLSMDQERLWFMHQLAPESTAFNIDAASRLRGALHLPSLHGALNEIVRRHAA
                     WRTTFAEVDGRPVQRVIPRRAQNLSIVDLSGLPADRREPTATRLLAEGTRELFDLERG
                     PLVRIRLLRLDAEDHICLMTVHHLVIDWISFQIVWTELAAFYDAGLTGRPAAVPEPPV
                     HYSDYAVWQREWLSGEVLDDLVEHWRRRLDGFPQVLELPTDRPRPPVQRMHGGSVDLD
                     TGRAWMEGLRGLSRREGATSFMGFLALTYALLHRFSGQEKMILGSNNANRNRPEVEPV
                     IGYFLTQVPFAVDLSGDPPFRELLGRVRQAALDAYAHQDLTFGLLVQALQPPRDTSRP
                     PLIQTLVLVLDGQYNENDLTGLAGEAVNVLDANARYDLMFGVYEQEDTIQGPIEYDAD
                     LFDHSTAERLIEVFVRMGEAVISDPTIRLSQLPTLSDAARHQVLREWNDTATPPALWT
                     VPALFAELAALTPDAPALVFAGETVTYDALDAHAKSLARRLDLVPESRVAILLERTPA
                     LPAALLAVWRAGGACVPLDPTSPLDRLRALLTDAAPVAVIHDGTLPAGILP"
     gene            complement(1876..>4153)
                     /locus_tag="VEL74_18430"
     CDS             complement(1876..>4153)
                     /locus_tag="VEL74_18430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64563.1"
                     /translation="RDLSRTPLFQVSLALQNMPLPEGGPEGLEMTPRELPAGTAKYDL
                     SLGLREQGGVFCAVLEYASDLFDRTTAHRLCEQWTRLLAAAVAAPATPLTELPLASEA
                     QLQQVLWEWSGAGRRLPEDIDGTLHGLFAARVAGSPDSPALVFEDHVVTYGELQARAE
                     RLASRLRSLGVGPEVVVGLLAGRSIEAVVGILGVLQAGGAWLPLDPSYPTERMALLLR
                     ETAAPVLLAEAGAALPEVEGQVVALDAPNSLTPSPITSPLPGEGEGTPDNLAYVLYTS
                     GSTGAPKGVAVPHRGVVNRLLRSRETYRIGPGDVMLQRAAGVFDVSVWELFGALASGA
                     RVGLPSQGLERDPRSLARLVAEQGVTLADFNPSALEAFLDQEDFAGAAASLRQVFAGG
                     EAVPAELPARLAGAGFRTPLANMYGPTEASIDLLWRSCAPGEQLPAGTVPIGRSIPGT
                     WAYVLDAQLSPLPVGIPGELWLGGVGLARGYLGRPDLTAERFLPDPFSPVPGGRLYRT
                     GDLARFLTDGEVQFLGRVDEQVKIRGYRVEPGEIEAELARLPGVREAAVAVREDVPGE
                     RRLVGYVVSSSTPDELRAALAVLGQRLPAWMVPPVFVTLESLPRTPGGKVDRRVLPAP
                     GALSAGVPAESFVAPRSDLERTIAAVWQEVLQLPRVGVQDNFFELGGSSLLLARLQSR
                     LEKALGRELPLMELFRHPTVEALARHLAGGEAGQARREGRGEQARARTQTRQEAMRQL
                     SQNRTRRRGPAGNGRGEG"
BASE COUNT          635 a         1482 c         1489 g          547 t
ORIGIN      
        1 tctgggagga tgccggcggg gagagtgccg tcgtggatca cggcgacggg ggcggcatcg
       61 gtcaggaggg ctcgtagacg gtccaggggg gaggtggggt ccagggggac gcaggcgcct
      121 ccggccctcc agacggcgag cagggcggcg ggcagcgcgg gggtgcgctc gaggaggatg
      181 gcgacgcggg actcggggac gaggtccagg cggcgggcca gggatttggc gtgagcgtcc
      241 aaggcgtcgt aggtgacggt ctctccggcg aagaccaggg ccggcgcgtc cggggtgagg
      301 gccgcgagct cggcgaagag ggcgggaacc gtccagaggg ccgggggtgt ggccgtgtcg
      361 ttccactcgc gcagcacctg atggcgggcc gcgtccgaga gggtgggcag ttgagaaagg
      421 cgaattgtgg gatcggagat caccgcctct cccatacgga cgaagacctc gatcaggcgc
      481 tcggcggtcg agtgatcgaa gaggtccgcg tcgtactcga tcggcccctg gatcgtatcc
      541 tcctgctcat agacgccgaa catgaggtcg tagcgggcgt tggcgtcgag gacgttgacc
      601 gcctcgccgg caagacccgt gaggtcgttc tcgttgtact gcccgtccag caccagaacg
      661 agcgtctgaa tgagcggcgg ccggctggtg tcgcggggcg gctggagcgc ctggaccagg
      721 agaccgaagg tcaggtcctg gtgggcgtag gcgtcgagcg ccgcctggcg cacccggccc
      781 agcaactcgc ggaagggggg atcgcccgac aggtcgacgg cgaaggggac ctgggtcagg
      841 aagtagccga tgaccggctc tacctccggc cggttgcggt tggcgttgtt ggagcccagg
      901 atcatcttct cctggcccga gaagcggtgc agcagcgcgt aggtcagggc caggaagccc
      961 atgaagctgg tagccccctc gcgccgcgac agcccgcgca gcccctccat ccacgcacgg
     1021 cccgtgtcca gatcgacgct gccgccgtgc atccgctgca ccggcgggcg tggccggtcg
     1081 gtcggcagct ccagcacctg gggaaacccg tcgagccggc gccgccagtg ctccaccaga
     1141 tcgtccagca cctcaccgct cagccactcg cgctgccaga cggcatagtc ggagtagtgc
     1201 accggcggct cgggcaccgc cgccggccgg ccggtcaggc ccgcgtcgta gaacgccgcc
     1261 agctcggtcc agacgatctg gaaggagatc cagtcgatca ctaggtggtg cacggtcatc
     1321 aggcagatgt ggtcctcggc gtccagccgc agcaggcgga tccgcaccag agggccgcgc
     1381 tcgaggtcga acagctcccg cgtcccctcg gccagcagcc gcgtggcggt cggctcgcgc
     1441 cggtctgcag gcaggccgga caggtcgacg atcgacaggt tctgcgcccg acgggggatg
     1501 acccgctgca ccggacggcc gtccacctcg gcgaaggtgg tgcgccaagc cgcgtgacgg
     1561 cggacgatct cgttcagggc gccatggagg gacggcaggt ggagcgcccc gcgcaggcgg
     1621 ctcgccgcgt cgatgttgaa cgccgtgctc tccggtgcga gctggtgcat gaaccagagc
     1681 ctctcctggt ccatcgacag gggccagtcc ccttcccctg tgggcccgga atggcggacc
     1741 accggcggcg gctggtgcac cttgggcgcc gcggccttcg acttcttctt gagcagctcg
     1801 aagagagccc gctgctctgg agacagtgaa gagatccggc tcgcgacact catacctcgt
     1861 gactcctttt gcctctcatc cttcacctcg gccattcccg gccggcccgc ggcgccgtgt
     1921 ccggttctgg ctgagctggc gcatcgcctc ctggcgcgtc tgggtgcggg cgcgggcctg
     1981 ctcccccctg ccctcccggc gcgcctgtcc agcttcgccg cccgccaggt gacgagccag
     2041 ggcctcaacc gtagggtgcc tgaagagctc catcaggggc agttcccgtc ccagcgcctt
     2101 ctccagacgg ctctggagcc tggcgaggag cagtgagctg ccccccagct cgaagaagtt
     2161 gtcctggact cccacccgcg ggagctgcag cacctcctgc cacacggcgg cgatggtcct
     2221 ctccaggtcg ctacgcggcg ccacgaagga ctcggccggc acccccgccg aaagtgcccc
     2281 gggtgcgggc aggacccggc ggtccacctt tccgccaggg gtgcgcggca ggctttccag
     2341 agtcacgaaa accggcggca ccatccaggc gggcagccgc tgcccgagga cggcgagggc
     2401 ggcgcgcagc tcgtccgggg tggaggacga caccacgtag cccaccagcc gccgctcgcc
     2461 agggacgtcc tcccgcaccg ccaccgccgc ctcgcgcaca ccgggaaggc gcgccagctc
     2521 cgcctcgatc tcccccggct ccacccgata gccgcggatc ttcacctgct catccacccg
     2581 gcccaggaac tggacctcgc catcggtcag gaagcgcgcc aggtcgcccg tccggtagag
     2641 ccgcccgcct ggcaccggcg agaacgggtc cggcaggaag cgctcggcgg tgaggtcggg
     2701 ccgcccgaga tagccgcggg ccagtccgac acctcccagc cacagctcgc cggggatgcc
     2761 cacggggagc ggcgagagct gggcgtccag cacataggcc caagtgcccg ggatggaccg
     2821 cccgatcggc accgtacccg cgggcagctg ctcccccggg gcacaggagc gccagaggag
     2881 gtcgatggac gcctccgtag gcccgtacat gtttgcaagc ggcgtccgga aaccggcgcc
     2941 ggcgagtcgg gcgggcagct ccgccggcac ggcctcgcca ccggcgaaca cctgccgcag
     3001 cgaggcggcg gcgcccgcga aatcctcctg atcgaggaac gcctctagag ccgaggggtt
     3061 gaagtccgcc agcgtcaccc cctgctccgc cacgaggcgt gccagggagc ggggatcgcg
     3121 ctccaggccc tgagaaggca gccccacccg ggcgccggac gcgagcgcgc cgaacagctc
     3181 ccacaccgag acgtcgaaga cccccgccgc tcgttgcagc atgacgtccc ccgggccaat
     3241 gcggtaggtc tcgcgtgacc ggagcaaccg gttgaccact ccccggtgcg gcaccgccac
     3301 ccccttggga gccccggtgg agccggaggt gtagagcacg taggcgaggt tgtcaggagt
     3361 cccttctccc tctcccggca ggggggaggt gatgggagag ggagtcaggg agttaggggc
     3421 atcgagtgcc accacctgcc cctccacctc tggcagcgcc gcccccgcct cggccagcag
     3481 caccggtgcc gcggtctctc tcagcagcag cgccatcctc tccgtcggat aagaggggtc
     3541 cagggggagc caggcgcctc cggcttggag gacgccgaga atccccacca ccgcctcaat
     3601 ggagcggccg gcgaggaggc ccaccacgac ctcgggcccc acgccgagac tccgcaggcg
     3661 gctggccaag cgctccgcgc gcgcttgcaa ctcgccatag gtcaccacgt ggtcctcgaa
     3721 gaccagtgcc ggggagtcgg gggatccggc tacccgggcc gcgaacagcc cgtggagcgt
     3781 gccgtcgatg tcctctggga gccgccggcc ggccccggac cactcccaga gcacctgctg
     3841 gagctgcgcc tcgctcgcca gaggcagctc cgtcaagggc gttgcgggag cggccacggc
     3901 cgcggcgagg agccgcgtcc actgctcgca gagtcgatgt gccgtggtgc ggtcgaagag
     3961 gtccgaggcg tactccagca ccgcgcagaa gactcccccc tgctcccgca gccccagcga
     4021 caggtcgtac ttggcggtgc ccgccggcag ctcccggggc gtcatctcca gcccctccgg
     4081 ccccccctcc ggcagcggca tgttctgcag cgccagcgac acctggaaga gcggcgtgcg
     4141 cgacaggtcg cgg
//
LOCUS       DASPJI010000654        27801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_722452, whole genome shotgun sequence.
ACCESSION   DASPJI010000654 DASPJI010000000
VERSION     DASPJI010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 27801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27801)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27801
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722452"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VEL74_18435"
     CDS             <1..373
                     /locus_tag="VEL74_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64564.1"
                     /translation="KTVTAAKKSEAKPKKTTAAKTVKGSTGAGAGAKKTATKSAAKSG
                     GTKTAAKSAAKPAAKKTAAGGEKKAAAKKTTAAKTTAAKKTTAAKKSDGAKKSDGGGA
                     KKTAAKKTAAKKAPAKKSSGS"
     gene            complement(594..1355)
                     /gene="pgl"
                     /locus_tag="VEL74_18440"
     CDS             complement(594..1355)
                     /gene="pgl"
                     /locus_tag="VEL74_18440"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HYG64565.1"
                     /translation="MEKLIDLRVVDGGDALARAAAEEVLRTAEAAVREHGRYTIALSG
                     GSTPKALYRLLASADGPFRARMPWERTHFFWGDERHVPPDHADSNYRMARETMLDSVP
                     VPAANIHRVPAEEPAEAAAAAYERELRELFQPVEGELPRFDFNLMGLGPDAHTASLFP
                     GTSVIHETERWVAAPWVEAHRTYRITFTPPVLNAAARILFLVSGGDKAAAVRAVIEGP
                     RDVDRYPGQVVKPARGTLLWLLDREAASLLSDTRS"
     gene            complement(1343..2875)
                     /gene="zwf"
                     /locus_tag="VEL74_18445"
     CDS             complement(1343..2875)
                     /gene="zwf"
                     /locus_tag="VEL74_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HYG64566.1"
                     /translation="MAIAEEVRIEDAEREGEPAPPCVMVIFGATGDLTKRKLVPALHN
                     LAMSGLLPDEFAVVGLGRGLTSDEQFREKIAGELKEFSPGPVDDAKSDWLMKRLFYMA
                     GEFTDPATYDGLRDLLAKIDQDLETGGSCLFYLAIPPSLFGEVVERLGAAGLTQESEG
                     AWRRVVIEKPFGHDLDSAKALNRQILQVLAEHQIYRIDHYLGKETVQNIMAFRFANGI
                     FEPIWNRRYVDHVQITVAETVGVEGRGAYYEEAGALRDMVQNHMFQLLAFTALEPPTS
                     FAGDAVRNERVKVLCAIRPMSHEDVLRQAVRGQYGEGTIDGKRVPAYRAEKNVAPNSP
                     VDSFVALELFIDNWRWADVPFYLRTGKRLPKRVTEIAIQFKRAPFMLFRETPVERLQP
                     NLLVLHIQPDEGISLRFEGKVPGPSMRLGTVKMDFQYADYFGTAPQTGYETLLYDVMT
                     GDSTLFHRSDMVEAGWSVVTPVLDVWKALTPRDFPNYAANSWGPRDADELIERSGRRW
                     KN"
     gene            complement(2907..3890)
                     /gene="gnd"
                     /locus_tag="VEL74_18450"
     CDS             complement(2907..3890)
                     /gene="gnd"
                     /locus_tag="VEL74_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HYG64567.1"
                     /translation="MQIGMIGLGRMGGNMVRRLMRDGHRCVVYDLNPQAVADLAGEGA
                     EGASSLDDFVARLETPRTAWLMVPAGAPTEGMVQELAGRLAPGDVVIDGGNSFFKDDV
                     RRARELAARQIHYLDVGTSGGIWGFERGYCLMIGGDPETVRRLDPIFKTLAPGRRDIP
                     PSPGREGRGGTAEEGYLHCGPAGSGHFVKMVHNGIEYGLMQAYAEGFDIFRNAASADL
                     PEEHRFDLDLADIAEVWRRGSVVSSWLLDLTASALAETPDLADYTGFVQDSGEGRWTI
                     NTAIEEAVPANVLSAALFTRFRSRQEHSFAEKVLSAMRKKFGGHVERPTGG"
     gene            4011..4718
                     /locus_tag="VEL74_18455"
     CDS             4011..4718
                     /locus_tag="VEL74_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64568.1"
                     /translation="MDKTRMVIAGTALAAALLLSPSPGRAQEESRVALGAKAGTLGAG
                     VELTVGLGRQVNTRLGFGAYSYSERREASDIEYDGEAKLRNGTAFLDWHPGGRGFRLT
                     GGLVYNDTEIQGESLTPASGVYNIGGVPVPASLVGRLEGTIDFDPIVPYAGLGWGNAV
                     RGGGPWGFSLDLGVVFQGEGDVTLTPLIPANSPLNDPLARELLAIQLEREAQDIEEDI
                     ADYDLYPVLSLGVTYRF"
     gene            complement(5047..6615)
                     /locus_tag="VEL74_18460"
     CDS             complement(5047..6615)
                     /locus_tag="VEL74_18460"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYG64569.1"
                     /translation="MGLNMDNGHPPAPWNRREAMVPAALALAVLALQLATADAYGWFR
                     DEFYYIACGEHLDWGYVDHPPLSAWIARFAREVFGESLLAIRLLPALVGAAVVLLAAR
                     LAREMGGGALAQALAALCVALAPVYLSLFHFFSMNSFEILFWTLGALVLARIVRTGDG
                     RLWLLFGLIAGIGLLNKHSMLVFGFAAFAGVALTPERRHFARPWIWLGGLLAFLLFLP
                     NLIWQHLHGWPTLEFMDNAQRLKNVDLAPLDFLREQLLMMNPLAAPVWLGGLGWLLLA
                     RQGRPFRPLGWLYLAALAVLLTQRSKPYYLAPVYTLLFAAGGVAWELWLQRLREPRLR
                     LVAAGLVVLPAAVGGLIGLPLAVPVLPVEGFIRYSRALGIQAESGERHEMGELPQLYA
                     DMHGWPEMVAEVARVYHSLPPEERAVAGIFGQNYGEAGAIGVLGKAYGLPPAISGHNS
                     HWLWGPGSYDGRVLIVLGGDEEGNRRACPDLRRAGTVRCRYCMPYEDELPVWVCRNVT
                     PPLSELWPRLKKYI"
     gene            6798..8018
                     /locus_tag="VEL74_18465"
     CDS             6798..8018
                     /locus_tag="VEL74_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64570.1"
                     /translation="MNAIAESYVKLVLAVGQHDPDYVDAYYGPPEWRTQAEQAKRPLA
                     EIRTEASGLLERLGATPPPAGGDEMERLRYEYLTRQLQSMAARVDMLAGKKMTFDEEA
                     RALYDAAPPTHGAAHFQAILDELAKVVPGDAPLPERVEVFRQRFVIPKDKLDAVFTAA
                     IDECRRRTAEHLELPPGESFEVEYVTGKSWSAYNWYKGNFHSLIQVNTDLPVFVDRAI
                     DLACHEGYPGHHVYNALLEKNLVKDRGWPEFSVYPLFSPQSLIAEGTANYGIAVAFPG
                     EERVAYERQVLFPLAGLDPSEAPRYYEMQRLLQRLNYAGNEAARRYLNGEIDGEAAAD
                     WLVRYSLTAPERARQRVRFFDQYRSYVINYNLGQDLVREYIEGRGGTEDNPGKRWEEF
                     EKLLSSPRLPSGLR"
     gene            complement(8025..8441)
                     /locus_tag="VEL74_18470"
     CDS             complement(8025..8441)
                     /locus_tag="VEL74_18470"
                     /inference="COORDINATES: protein motif:HMM:NF013158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64571.1"
                     /translation="MLRSTTLALTLILALTGADAALACSCAPSPPPQEALQQAGAVFT
                     GTVVAVDTSGLSSTVRLRVEKGWKGAKCGEVTVVTSADSAACGYDFQVGQSYLVYADK
                     QKGKLNVSLCSRTSLTSQAGEDLAALGEPATTCPAQ"
     gene            complement(8538..10640)
                     /locus_tag="VEL74_18475"
     CDS             complement(8538..10640)
                     /locus_tag="VEL74_18475"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HYG64572.1"
                     /translation="MSFGRQKRLLLGWLAFFAPIPLPFNDLVLWPVLAVYLAGVLVFL
                     RRAGQDAPRWLPRWGMNLLGLAYLPIFFLDLTAFSRGRLVQPAVHLCLFALLVKLFSL
                     ERERDKWQAAIGIFFIFLAAMGTSVHPAVVLYLVGFMVVSLVLLSRFAFLHVLSGFGR
                     DDPALARIPMRGFLWVSALAAVLLAVPIFALLPRVRAPFIVGRGMSTGTTLEAAGFSD
                     EVTLDSIGNIRSSREVALRLVQEGGQFDPDEEVRFKAATYDVYQGGSWRRSPQVGPLR
                     REQGIRFPLSREEPLRWVRIWLQPMRSRSLPLPVQSAIVEPRTPRLEIDQGGAVSLSF
                     DPVEIIEYRVGLAGQPVLLGKVPGPAPEPTLDLSGVTPRISGLAARVMGEGPAGERAY
                     RLETHLVQSYSYTLDFRGRSSDNPIEEFLFRYRSGQCEYFASSMVLMLRSQGIPARLV
                     TGFLGGEHNPFEGYYIVRDSNAHAWVEAYLPGTGWRTFDPTPPAGRPETGQEGNWLLA
                     QQAWDYLLFRWDRYVLTFGLYDQIRLFSGLREVWSDFWGMFQRKQQEERPAGGAATDA
                     APGGEAGAGESPPLPASTAMWALAGLVLLAAAAWLLARRLRPPLTATTAYRRLRHRLA
                     RAGAPFPESFPPLALSRETAARYPAAAQPAARVIELYLRESFGGQDLRDDERQALEAA
                     LAEAEEGLKAERKLKKAS"
     gene            complement(10637..11665)
                     /locus_tag="VEL74_18480"
     CDS             complement(10637..11665)
                     /locus_tag="VEL74_18480"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYG64573.1"
                     /translation="MSWKRTSTPEGIRITTVGLWYVLLSILVGITATNTGNNALYTVL
                     AVMFAVLIVSGVMSRENVRGLEAELEPPGEVFANRPFAIGFSLRNRSRLFPRWFLLFT
                     VSRSAQPLLIPYLPRRGRSSGSIDMIVPSRGRQSLPFAHVSSLFPYGFFRKGVRYRVD
                     LELLVFPEIFAAAASRLDEVDHMGEEASRRTGWGHGLHSLRQFRRGDDPRGIHWKQTA
                     RTGELVYMERESERTRRLSILFDNAVGELADPESQKRFERLVSEAATAAVDHLARGYE
                     VELVTHDQHLPFAGGARQRLAVLESLALVEARPRAGEVLLPGDHRAAQLRVHMEPEGA
                     PEPKEVAV"
     gene            complement(11684..12655)
                     /locus_tag="VEL74_18485"
     CDS             complement(11684..12655)
                     /locus_tag="VEL74_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HYG64574.1"
                     /translation="MEPILIDAADAANAPGAVEPIRTLEDNVARVVRGKAEAIRMAAV
                     CLLARGHVLVEDVPGVGKTTLAQALARSLGLAFQRIQFTSDLLPSDVIGISIFNQKNQ
                     AFEFVPGPLFANLVLADEINRATPKTQSALLEAMSERKVSIERKRYTLPEPFIVLATQ
                     NPVEYQGTFPLPESQLDRFLMSISLGYPPRADEKELLLSGGVEDLLEHLQPVLSREGL
                     LALQQQVRLVRVADKLAEYMLSLAEATRKGGDFLVGVSTRGVQSLYRATQALALCEGR
                     SYAVPDDVQRLAVPVLGHRVVLKRGGAGLEMARKAIERVVAATPVPL"
     gene            12917..13945
                     /gene="recA"
                     /locus_tag="VEL74_18490"
     CDS             12917..13945
                     /gene="recA"
                     /locus_tag="VEL74_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HYG64575.1"
                     /translation="MATDLKADRLKSIEGALSQIERQFGKGAIMRLGQREALAIEAIS
                     SGSITVDHAIGIGGFPRGRVVEIYGPESSGKTTLALSTVGQAQKRGGTAAFIDAEHAL
                     DAEYARKLGVDIDNLLVSQPDNGEQALEIAEMLVRSNAVDVVVVDSVAALVPKAELEG
                     EMGDSHVGLQARLMSQALRKLTAIVSKSRTCLVFINQIREKIGVMFGNPETTTGGRAL
                     KFYASVRVDIRRIASLKDGEEVTGARAKVKVVKNKVAAPFRQAEFDIDYGEGISKTGE
                     LVDLGVEHKLMVKSGAWYSYGDLRVGQGRDNAKQFLRDNPDLADEIEGKLREKMGMPP
                     VAAVPKDE"
     gene            complement(14008..15120)
                     /locus_tag="VEL74_18495"
     CDS             complement(14008..15120)
                     /locus_tag="VEL74_18495"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HYG64576.1"
                     /translation="MEFSGRLAAFPMGDLLQWAQNERCTGALVVRRSQREKRIYFHLG
                     EVVSCLSNDPAEFYGQHLLLHGHVGQDALFQALSHCTTRRTRLGVALRELGLLSPEVI
                     QQTLRRQIEDSICDLFLWRGGVFYFQSELPQEEEILTEPIHSMGLVMEGTRWVDEHAR
                     IRRVFVHDNVVLRRGDAWPGEGLEPMERRLAGLVDGKKNLADLYEQLKGSYFRFLEAA
                     LRLTVAMVLDIEKVGEANETGTRELSVFDLLLEQATEEQVLVARRHMAVPLDLLERCY
                     PVWVLEPTTDERQRIPMRARDFYARFDGRTALGEAFSGDPRQRGRELDLLMLQLQKGS
                     LALLPAPLEKIEKEADQRGQPPIQRWWRRFFPQAAG"
     gene            15234..18308
                     /locus_tag="VEL74_18500"
     CDS             15234..18308
                     /locus_tag="VEL74_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64577.1"
                     /translation="MSIAAAMAAAPLPCQPCAGVRLEPLQPQAAPAAPSPAETGGVSQ
                     GSQPVQIPEGNPTEIQPQQLPPMVAGPDSLPTVPSEIGALLQQSPKLEPGSPLFVAWD
                     VLLGEEAAPVSVGAEALGIVQTGGTPWLSLVFRTPAPVTQNVARLQAELRGAAAIAAA
                     APANTWFQVAWRPEGAETQAFAPGEYAFLLKRAAVALTGARADAKVASAPLPADPAQI
                     RAFYGEEVAAYLEAVALQPAPAAALTAAVAALQELDPGRPVVLDAVDYPADPSGVLSE
                     AARQSARGIGLTLFRKAKLTAADLAPLALLARELAGDFTYDPASTPTGAGEAWTFIGA
                     ASDAGGAGDTLELRVIAEAPAGASELTLRFPDAYLRRPTRFPYTPGRFDPPSGRVEGD
                     ALLLTVTNPGRVEVVGLERATAAEREGVEERVTIATEREMPVEEILRRLQAFEDAQAR
                     RLDHYRATNTTHMRFQPAAGTQSIEATLQGPFFFDPQTGSDWAWEALYVNGVRWRGKS
                     IPEIPLVQPEKAAALPLEIHFNKQYRYRLRGTDTLDGRPAWVVDFAPTGPAAEGGKLY
                     QGTVWVDRQIYSRLRTRAVQLGLEGEVISNEETMHYTPVDAEGKPAPWGPQSYVLPLR
                     IVAQQLLSVVNSATVVERETLLTDVRINAAGFEEARRQVAGTDVTMVRDTAEGLRYLV
                     KDDSGERVVQEGFDTSKLFALGGVFYDDALDYPLPLAGVNYFSFDFRGTGKQVNAFFA
                     GALLTADIAEPRLFGSRFDAGVDLFALAIPLTDTVYRGDREANEEDVELRTGNIGFKI
                     GHPVGNFMKLGAEYTPLFFNYGRADDTDPNFVIPEDHVLHALDLRASFNRSGYGFSLT
                     GSYNKRSEWKPWGLPGNPEYSEDHEDFLRWEARASKNWYLRSFQKVGIEFDYASGQDL
                     DRFSKYQFGFFGGTRVHGYQSNRVRATEVLASHVSYGFEVGQMLRLEALGDVAWATDE
                     DTGLDNELLGGLGIAGTFMGPWQTVVNLDLGVPVAGPDDGFVVYVVFLKLFN"
     gene            18318..18611
                     /locus_tag="VEL74_18505"
     CDS             18318..18611
                     /locus_tag="VEL74_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HYG64578.1"
                     /translation="MEDEKVWEDPAEPVELPITDILDLHSFRPSEVADVVREYLDAAY
                     EKGLRDVRIIHGKGIGVQRQTVRTILGRDPRVEACGDLPGEAGGWGATWVRMR"
     gene            complement(18608..19054)
                     /gene="aac(6')"
                     /locus_tag="VEL74_18510"
     CDS             complement(18608..19054)
                     /gene="aac(6')"
                     /locus_tag="VEL74_18510"
                     /EC_number="2.3.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010223087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047663 - aminoglycoside
                     6'-N-acetyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6'-N-acetyltransferase"
                     /protein_id="HYG64579.1"
                     /translation="MIEHCTSVEQPGWLELRMALWPEGTPEQHREEMASFLEQPGRYV
                     QVVAYGPDREPLGFVEASVRTDYVNGTESSPVAFLEGMYVVPEARGQGVAREMVGAVE
                     AWGWEMGCSELASDAYLDNELSHAVHRALGFEETERVVYFRKELKG"
     gene            complement(19080..19436)
                     /locus_tag="VEL74_18515"
     CDS             complement(19080..19436)
                     /locus_tag="VEL74_18515"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HYG64580.1"
                     /translation="MRFLLDEDVNPKVAEIARGLGLDAVSVHEISRRGFTDPDQFRFS
                     AAQGRIFVTRNRDDFVHLTVTFYEDGEPSPGLLVIPRSLPNSQPERIAHALNRWAEAR
                     RDWEEAWDYGVDFLAP"
     gene            complement(19436..19864)
                     /locus_tag="VEL74_18520"
     CDS             complement(19436..19864)
                     /locus_tag="VEL74_18520"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HYG64581.1"
                     /translation="MAEPVRAIQVPSNLEREIEREAEQRGKSWSATTVELLEEAIRMR
                     RVPGISFSEGPAGRRAGVAGSGLDVWEVVATWRQVGQDLGRLRQEYPWLTEPQLRAAL
                     TYYELYPGEIDARLAREEEWTPAKVSSELARAKQKGPRQG"
     gene            19998..21863
                     /locus_tag="VEL74_18525"
     CDS             19998..21863
                     /locus_tag="VEL74_18525"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HYG64582.1"
                     /translation="MDSPWRSFKKLLRRGRLRLAPHAGWPRVDFVYGREYQTDLPAVS
                     HDSRRGERILSFLDWEGLLDRRFLHHPEPATYRLLRRVHTDEYLESLNQTATLTPIVG
                     FDMAERAAERLVEGQRAMAGGTVLAARLALESKGIAANLGGGFHHAFAGKGARFCLFN
                     DVAAAITDLRDNGFGGPILTVDLDLHDGDGTRSLFARDPTVYTFSIHNQSNPQPGEEA
                     VASTAIELGGGVDDKTYLDAIRAHLPPVFASFRPELVIYLAGCDPAAGDEIGDWKISS
                     QGMLERDRFVLHLSRGLGRSGPRRLPLVIVLAGGYGQRSWRYSARFLSYLLLGRPVEP
                     PTTDESVVMLYRQLARGYGEPELTSEPAASEDGADWGLTDEDIGMALGGPRRPRRLLG
                     YYTPQGLELALERTGFLDRLRALGFPRPVAELDLDNPTGDTVRFWADRRKADLLIEVR
                     MRVDRGTAPDLALLRAEWLLLQNPHAQPTPERPLLPGQRHPGLGMLQDVMALMVLVCD
                     RLQLDGVLFVPAHYHTAAYGRKLMRSLDPVDEARLRALRAVLDRLPLSEATHAMESGR
                     VIDTHTGDPAVWQPRPLVYPVSQRLRERLEAGDYERQVAEAAPRFGFALRQAANG"
     gene            complement(21845..23113)
                     /locus_tag="VEL74_18530"
     CDS             complement(21845..23113)
                     /locus_tag="VEL74_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64583.1"
                     /translation="MPRIHPKPEVIDRLLELVPESRNRVLLHLCECPRCHELWLGPEE
                     GEGGGEPVGARVLAWRPPETEYEIVVDRVLRGFQSHFHSVERERAAAPGLLEELRRHP
                     PERRAWLIAERTRFRTLALVELLVRTSDEEVHRDAGEGERWGELALRLADRLDPGRHG
                     TRVVEDARARCWASIGNARRVATDLLGADQAFRAAEAHLRQGSRDPLQRAPVLAYKAS
                     LRRAQRRFGEAISLLKRSISISLSAGDLRRVLTSLLVWVRVCQETGEPEEAVRLLRVA
                     NGLAGPDLDPRLKIAIRHNLLVSLMDLGRLREARALLVRSRALYRQVSDSMIQLRMRW
                     LEAQILAGLGQLPRAVLLLDRVRSGFAQLGNGYEAALVSLELAALHARLGRPVVARRL
                     AVEAFPIFRSRGVQREALAALILLVQPLAA"
     gene            23197..24663
                     /locus_tag="VEL74_18535"
     CDS             23197..24663
                     /locus_tag="VEL74_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HYG64584.1"
                     /translation="MTESVSLPLWLVLLLVLLAGWAVLERLLVPSVRWYLRRRVNRVI
                     EELNTRLRVEIRPFQRTRRQVLIDRLIYDPKVVEAVGARAREQGMPRDVVMAEVERYA
                     REIVPAFNAYFYFRIGYWISRAVTRLLYRVRLGFSDEAGLAEVAPHSAIVFLMNHRSN
                     MDYVLVSYLAADRTALSYAVGEWARIWPLQTLIRATGAYFVRRGSGNPLYRRVLERYI
                     HMATAAGVTQAVYPEGGLSRDGHLRPPKLGILDYMLRGFDPQGERDLVFIPVGINYDR
                     VLEDRTLLLDVQPPPEDRKGPLAAAATASGFALRHLGLMLAGRWHRFGYACVNFGTPL
                     SMRRWLGERRLDLRIVSREERSAALEELGGELMERVGSVVPALPVPLVASVFVQRLDR
                     LDGEMGGPLSELELKARVYRLMGELEAGGAHVYIPRSDQDYAISVGLRTLIQRHLVDE
                     RGGLFAPRETEIAVLRYYANSIAHLIPETPAQPARITS"
     gene            24665..25831
                     /locus_tag="VEL74_18540"
     CDS             24665..25831
                     /locus_tag="VEL74_18540"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HYG64585.1"
                     /translation="MRASVTPPPLRRFERFVRDYTDGLNTRDLQRLFQGDAARAYAVL
                     TREHGQQPEPKDGLRRLLHRIRLSFLGLSYKLSPARRFLFALSVLFFLMGCFFDGAIQ
                     VDTGTTRYAVGFSPVYFSVSFGLLVYLLALELVDRVRVRDELQVARELQADLLPREVP
                     PVPGYSFAHSYRTANEIGGDYYDFTPLPDGRVAITIGDASGHGMAAGLVMAIANATLE
                     TALDLDPTPERVVSLLNRTLCRTGSRRTFMSLFFALLDPATGRLDYVCAGHPFPLLRR
                     VGGAIEELGCGGFPLGIREPAGPVCKTTFLEPGDLLVLYTDGLAEAVNPQGTEAFSFD
                     RVRSLTSVGGSARDVHDWILKAFDRHVAGEPLKDDLTLVVLHRSPFPVPPPVPL"
     gene            complement(25888..26964)
                     /locus_tag="VEL74_18545"
     CDS             complement(25888..26964)
                     /locus_tag="VEL74_18545"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HYG64586.1"
                     /translation="MLVNGKLVRQSSHHWLPWLTLALLAVVLAPPAQAQPFGGYLDLS
                     GDGTSGPPTHGYIEIPHSPALNPTSALTIEMWVKLDTPQPGCVSLFGKDYTEAYWIGV
                     CGSTLRSYLKGSGSVRDGGTILDNAWTHIAVVFNGTTRLHYVNGVQVGAFAESAPLTT
                     STDNVRIGSDASYFFSPNGSIDEVRLWNVARTAAQIESTMRTPITTPTTGLVAVWSLD
                     GNGNDIIGGHDGTVSGNTSFGPVTPPAGPWLSSSDMPDFRFKVRLTTGGVSRMATQVA
                     DCIEQTVCAAGVFPDRPEVLLRVIGPRPNGYLWPIVIRFTNARVEVWVEQLSSGDIKY
                     YDLPFVGPDSSELNGINDRFGFQP"
     gene            complement(27424..>27801)
                     /locus_tag="VEL74_18550"
     CDS             complement(27424..>27801)
                     /locus_tag="VEL74_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64587.1"
                     /translation="LGVEAVFGVEPRGATAVLFTSVVDLAAAELLREALLRLGRRHRS
                     LLVNLEDPELRALADDEPATPEQAFAQAASLEIVLANRRLARHLRHGGIRVVGSPADR
                     LALETLEAYLAFYRGRSAPALTA"
BASE COUNT         4257 a         9958 c         9535 g         4051 t
ORIGIN      
        1 caagacggtg accgcggcca agaaatccga ggccaagccg aagaagacca ccgcggccaa
       61 gaccgtcaag ggcagcacgg gtgccggcgc cggcgccaag aagaccgcca ccaagtccgc
      121 cgcgaaatcc ggtggtacca agaccgccgc gaagtccgct gcgaagccgg ccgccaagaa
      181 gacggctgcc ggtggcgaga agaaggccgc ggccaagaag acgaccgcgg ccaagacgac
      241 cgccgccaag aagaccactg ccgcgaagaa gtccgatggc gcgaagaagt ccgatggcgg
      301 gggcgccaag aagaccgccg cgaagaagac cgctgccaag aaggctcccg cgaagaagtc
      361 gtccggcagc tagcgtgacc gaccggcgga ggccctcctc cgccaggccg gaagtggtct
      421 acggctcttt cgaaggcggc gctcggtcaa cttttcgggg cgagtttcct tgtcgtcctt
      481 ggagtctttc gaagcttcca gcgtcgcctc cgcacgacgt atcagcctcg ccgccccgcg
      541 gctcgagcgt cgtgtccact cctacccacc acaacccgac gtccggcgag atctcaggat
      601 ctcgtgtcac tgagcaggct ggccgcctcg cggtccagca gccagagaag ggtgccccgc
      661 gcgggcttca ccacctgtcc cggatagcgg tcgacgtcgc gcgggccttc gatcaccgcg
      721 cgtaccgccg cggccttgtc gccgccgctc accaggaaga ggatccgcgc cgcggcgttc
      781 agcaccggtg gtgtgaaggt gatccgatag gtgcgatggg cctccaccca cggcgccgcg
      841 acccagcgct ccgtctcgtg gatcaccgat gtgccgggaa agagcgaggc ggtgtgggcg
      901 tcgggcccca ggcccatgag attgaaatcg aagcgcggca actcgccttc caccggctga
      961 aagagctctc tcagctcccg ctcgtaagcc gcggcggcgg cctcggcagg ctcctcggcg
     1021 ggcacgcggt ggatgttcgc cgcgggcacc ggcacgctgt cgagcatcgt ctcgcgcgcc
     1081 atgcggtagt tgctgtcggc gtgatcgggc ggtacgtggc gctcgtcccc ccagaagaag
     1141 tgggtgcgtt cccacggcat gcgcgcgcgg aaaggcccgt ccgcgctggc gaggagccgg
     1201 tagagagcct tcggcgtcga gcctccggag agtgcgatgg tgtagcggcc gtgctcgcgc
     1261 acggccgctt cggcggtgcg caggacctcc tccgcggccg cgcgcgccag cgcgtccccg
     1321 ccgtccacca cgcgcagatc gatcaatttt tccaccgccg gccgctcctc tcgatcagct
     1381 cgtccgcgtc cctggggccc caggagttgg ccgcgtagtt cgggaaatcg cgcggcgtca
     1441 gggctttcca cacgtcgagg accggcgtca ccacgctcca tccggcctcc accatgtcgc
     1501 tgcggtggaa gagcgtcgag tcgccggtca tcacgtcata gagcagcgtc tcgtagccgg
     1561 tctgcggcgc ggtgccgaag tagtcggcat actggaagtc catcttgacc gtgccgaggc
     1621 gcatggaggg cccggggacc ttgccttcga aacgcagcga gatcccttcg tccggctgga
     1681 tgtggagcac cagcagattg ggctggaggc gctcgaccgg cgtctcgcgg aagagcatga
     1741 acggcgcgcg cttgaactgg atggcgatct cggtgacgcg cttcgggagc cgcttgccgg
     1801 tgcgcagata gaaaggcacg tcggcccacc gccagttgtc gatgaacagc tcgagcgcga
     1861 cgaacgaatc caccggcgaa ttcggcgcca cgttcttctc ggcgcgatag gccggcaccc
     1921 gcttgccatc gatggtgccc tcgccgtact ggccgcgcac ggcctggcgc agcacgtcct
     1981 cgtgcgacat ggggcggatg gcgcagagca ccttcacccg ttcgttgcgc accgcgtcgc
     2041 cggcgaagga ggtggggggc tccagggcgg tgaaggcgag gagctgaaac atgtggttct
     2101 gcaccatgtc gcgcagcgcg cccgcctcct cgtagtaggc gccgcgccct tcgacgccca
     2161 ccgtctcggc gacggtgatc tgcacgtgat cgacgtagcg gcggttccag atcggctcga
     2221 agatgccgtt ggcgaagcgg aacgccatga tgttctggac cgtctccttg cccagatagt
     2281 gatcgatgcg gtagatctga tgctccgcga gcacctggag gatctgccgg ttgagcgcct
     2341 tggccgagtc gaggtcgtgg ccgaagggct tttcgatcac cacccggcgc caggcccctt
     2401 cgctctcctg ggtgagcccc gcggcgccga ggcgctccac cacttcgccg aacaggctgg
     2461 gcgggatggc gagatagaac aggcaggagc cgccggtctc gagatcctga tcgatcttcg
     2521 ccaggagatc gcgcaggccg tcgtaggtcg ccgggtcggt gaactcgccg gccatgtaga
     2581 acaggcgttt catcagccag tcgctcttcg cgtcgtccac cggcccgggg gagaactcct
     2641 tgagctcgcc ggcgatcttc tcgcggaact gctcgtcgga ggtgaggccg cggccgaggc
     2701 cgacgacggc gaactcgtcg ggcaggagcc cgctcatcgc caggttgtgg agcgccggca
     2761 ccagcttgcg cttggtcaga tcgccggtgg ctccgaagat caccatcaca cagggaggag
     2821 cgggctcccc ttcgcgctcc gcgtcttcga tccgtacctc ctccgctatc gccatgaatc
     2881 ctcccctcga aagtcgagaa cgtttctcag ccgccggtgg gccgctcgac gtggccgccg
     2941 aacttcttgc gcatcgccga caggaccttc tccgcgaagc tgtgctcctg gcgcgaacgg
     3001 aagcgggtga agagcgccgc cgacagcacg ttggcgggca ccgcctcctc gatggcggtg
     3061 ttgatcgtcc agcgcccctc gcccgagtcc tggacgaagc cggtgtagtc cgcgaggtcc
     3121 ggcgtctcgg cgagggcgct ggcggtcagg tccagcagcc aggacgagac gacgctgccg
     3181 cggcgccaga cctcggcgat gtcggcgagg tcgagatcga aacggtgctc ctcgggcagg
     3241 tcggccgagg cggcgttgcg gaagatgtcg aagccttccg cataggcctg catcaggccg
     3301 tactcgatgc cgttgtggac catcttgacg aagtggccgg agcccgccgg cccgcagtgg
     3361 agatagccct cctcggcggt gccgccgcgc ccttcacgcc ccggcgaggg cgggatgtcg
     3421 cggcggcccg gggccagggt cttgaagatg ggatcgagcc gccggacggt ctccgggtcg
     3481 ccgccgatca tcaggcaata gccgcgctcg aacccccaga tgccgccgct ggtgcccacg
     3541 tccagatagt ggatctgccg cgccgccagc tcgcgggcgc gccgcacgtc gtccttgaaa
     3601 aaggagttgc cgccgtcgat caccacatcg cccggcgcga ggcggccggc cagctcctgg
     3661 accatgccct cggtgggggc gcccgcgggc accatgagcc acgcggtgcg cggcgtctcc
     3721 agcctggcga cgaagtcgtc cagtgaggag gccccttcgg cgccttcgcc ggcgaggtcc
     3781 gccaccgcct ggggattgag gtcgtagacc acgcaccggt gcccgtcccg catcaggcgc
     3841 cgcaccatgt tgccgcccat ccgccccagc ccgatcattc caatctgcat gctctccctc
     3901 ctctctgcca ggcgacagtc tatatcgcga tatcggcggg gatcggccgc cggcccctga
     3961 tttgcagtct tctccggcga actgcgagca tgcggtggaa agggatccct atggacaaga
     4021 cacgaatggt gatcgcgggt acggcgctgg ccgccgccct tcttctctcc ccctctcccg
     4081 gacgggccca ggaagagtcc agggtggccc tgggcgccaa ggcggggacg ctgggggccg
     4141 gcgtggaact gacggtgggg ctcggccggc aggtcaacac ccgcctcggc ttcggcgcct
     4201 acagctactc ggagcgccgc gaggcgagcg acatcgagta cgacggcgag gcgaagctgc
     4261 gcaacggcac cgccttcctc gactggcacc ccggcgggcg cggcttcagg ctcaccggcg
     4321 gcctggtcta caacgacacc gagatccagg gggagagcct gacgccggcg tcgggcgtct
     4381 acaacatcgg cggcgtgccg gtgcccgcct ccctggtcgg ccggctcgaa ggcaccatcg
     4441 acttcgaccc gatcgtcccc tacgccgggc tcggctgggg caacgcggtg cggggcggcg
     4501 gcccgtgggg cttctccctc gacctcggcg tcgtgttcca gggagagggg gacgtcaccc
     4561 tcacgccgtt gattcccgcc aacagccctc tcaacgatcc tcttgcccgc gagcttctcg
     4621 ccatccagct cgagcgggag gcgcaggaca tcgaggagga catcgcggac tacgatctct
     4681 acccggtcct gtcgctgggg gtgacgtacc gcttctgagc gcccggcatc cccgccccgg
     4741 cggtgattcc ccccggaaac cggccgccat ccccggcggc acgaaggcca tacccgcggc
     4801 gccaacccca tccccgcggg tacgaccctc atacccaggg tatggaggcg atctggcggg
     4861 gtacggtctc catacccgcg ggtaggaggt catccccggg gagatccccg ttctaccctc
     4921 ggggaggacg gccgtacccc gggtagggcg aacgtttccc ggggtatggc gcccctccct
     4981 cgtggggcaa cggctgcccc gggaggaagg acgcccgccc cgcgtgacgt cgatcgtgta
     5041 tcggggctag atgtacttct tgaggcgcgg ccacagctcc gagagcggcg gcgtcacgtt
     5101 ccggcagacc cacaccggca gctcgtcctc gtagggcatg cagtagcggc aacggacggt
     5161 gccggcacgg cgcagatcgg ggcaggcccg ccggttgccc tcttcgtcgc cgcccaggac
     5221 gatgaggacc cggccgtcgt agctccccgg cccccagagc cagtggctgt tgtggccgct
     5281 gatcgcgggc gggaggccgt aggccttccc gagcacgccg atggcgccgg cctcgccgta
     5341 gttctggccg aagatccccg ccaccgcccg ctcctccggc ggcagcgagt gatagacccg
     5401 cgccacctcg gccaccatct ccggccagcc gtgcatgtcg gcatagagct gcggcagctc
     5461 ccccatctcg tgccgctccc cggactcggc ctggatcccg agcgcccggg agtagcggat
     5521 gaagccctcc accggcagga cggggaccgc caggggaagg ccgatcaatc cccccaccgc
     5581 ggccggcagg accaccagcc cggcggccac gagccgcagg cgcggctcac gtagacgctg
     5641 cagccagagc tcccaggcca cgccccctgc cgcgaagaga agggtgtaga cgggagccag
     5701 gtagtagggc ttggaacgtt gggtgagcag caccgccagc gcggccagat agagccagcc
     5761 gagcggccgg aacggcctcc cctgcctcgc aagcagcagc cagccgagcc ctcccaacca
     5821 gaccggcgcc gcgagggggt tcatcatcag gagctgctca cggaggaagt ccagcggggc
     5881 caggtccacg ttcttgagcc gctgcgcgtt gtccatgaac tccagggtcg gccagccgtg
     5941 gaggtgctgc cagatcaggt tcggcaggaa gaggaggaag gcgaggaggc cgcccagcca
     6001 gatccacggc cgggcgaagt gccggcgctc cggcgtgagc gccaccccgg cgaaggcggc
     6061 gaagccgaag accagcatgc tgtgcttgtt cagcaggccg atcccggcga tcaggccgaa
     6121 gagcagccag agccgcccgt ccccggtgcg gacgatgcgc gccaggacca gcgccccgag
     6181 agtccagaac aggatctcga aggagttcat ggagaagaaa tggaagaggc tcaggtagac
     6241 cggcgccagg gccacacaga gagcggcgag cgcctgggcg agggcgccgc cacccatctc
     6301 ccgggcgagc cgcgccgcga gcaggaccac cgcggcgccc accagcgcgg gcaggagccg
     6361 gatcgccagc agcgactcgc cgaagacctc gcgcgcgaag cgggcgatcc aggcgctcag
     6421 cggcgggtga tcgacgtagc cccagtccag gtgctcgccg caggcgatgt agtagaactc
     6481 gtcgcggaac cagccgtagg cgtcggcggt ggcaagctgc agcgcgagca ccgccagcgc
     6541 cagtgccgcg gggaccatcg cctctcgccg gttccagggt gccggcgggt gtccgttgtc
     6601 catgttcagc cccatgctaa gcttccggcg ctccgccgga gcggtgcccg aaccgagcct
     6661 ccagaggatc tttcatgact cgatccatgc tccttgccct tctcttgacc tcgtccatgt
     6721 tggggggctg cgcggcccgc ttgcatcagc agccaacggg cggccatcca cagtccaccc
     6781 ctgagggacc ttcgccgatg aacgctatcg ccgaatccta cgtcaagctg gtcctcgccg
     6841 tcgggcagca cgaccccgac tatgtcgacg cctactacgg cccgccggag tggcggaccc
     6901 aggccgagca ggccaagcgc cctctggccg agatccggac cgaggcgtcc ggcctcctcg
     6961 agcggctcgg cgccacgccc ccgccggcag ggggcgacga gatggagcgc ctgcgctacg
     7021 agtatctcac ccgccagctc cagtccatgg cggcgcgcgt cgacatgctg gcggggaaga
     7081 agatgacgtt cgacgaggag gcccgggccc tctacgacgc cgcgccgccg acgcacggcg
     7141 ccgcgcactt ccaggcgatc cttgacgagc tcgcgaaagt ggtaccgggc gacgcgccgc
     7201 tgcccgagcg ggtggaggtc ttccggcagc gcttcgtcat ccccaaggac aagctcgacg
     7261 cggtgttcac cgccgccatc gacgaatgcc gccggcgcac cgccgagcac ctcgagctgc
     7321 cgccgggcga gagcttcgag gtcgagtacg tcaccggcaa gtcgtggagc gcctacaact
     7381 ggtacaaggg caacttccac agcctgatcc aggtcaacac cgacctgccg gtcttcgtcg
     7441 accgggccat cgacctggcc tgccacgagg gctatcccgg ccatcatgtc tacaacgccc
     7501 tgctggagaa gaacctggtc aaggaccggg gctggcccga gttctccgtc tacccgctct
     7561 tctcgccgca gtcgctgatc gccgagggga cggcgaacta cggcatcgcg gtcgccttcc
     7621 cgggcgagga gcgggtggcc tatgagcgcc aggtcctctt tcccctggcg ggcctggatc
     7681 cctccgaggc gccgcggtac tatgagatgc agcgcctgct ccagcgcctc aactacgcgg
     7741 gcaacgaggc ggcgcggcgt tatctgaatg gcgagatcga cggcgaggcg gcggcggact
     7801 ggctggtgcg ctactcgctg accgccccgg agcgggcgcg gcagcgggtg cgcttcttcg
     7861 atcagtaccg gagctacgtc atcaactaca acctggggca ggatctggtc cgcgagtaca
     7921 tcgagggccg gggcggcacc gaggacaacc ccgggaagcg ctgggaggag ttcgagaagc
     7981 tcctctcctc cccgcgcctc ccgtccgggc tgcgctaggc aggtctactg ggcggggcag
     8041 gtcgtcgccg gctctccgag agcggcgagg tcctcgcccg cctgggaggt caggctcgtc
     8101 cgcgagcaga ggctgacgtt gagcttcccc ttctgcttgt cggcgtagac cagatagctc
     8161 tgccccacct ggaagtcata gccgcacgcg gcgctgtcgg cggaggtcac taccgtcacc
     8221 tcgccgcact tggccccctt ccaccccttc tccacccgca ggcggacggt gctgctgagg
     8281 cccgaggtgt cgacggccac caccgtgccg gtgaagacgg cgccggcctg ctggagagcc
     8341 tcctgcggcg gcggcgacgg cgcgcaggag caggccagtg cggcatcggc cccggtgaga
     8401 gcgaggatga gggtgagggc tagcgtggta ctcctgagca tggtggatct cctggtcgaa
     8461 tggacgctcc ggccggaggg accggcggaa atattcgcta gcttctattt ggatgcaaga
     8521 cgtgtgcgcg gggcgcttca actggccttc ttcagcttcc gctccgcctt gaggccctcc
     8581 tcggcctcgg cgagcgccgc ctccagggcc tgccgctcgt cgtcgcgcag gtcctggccg
     8641 ccgaagctct cccgcagata gagctcgatg acccgcgccg cgggctgggc ggcggccggg
     8701 tagcgggcgg ccgtctcgcg gctcagcgcg agcggcggga acgactccgg gaagggcgcg
     8761 ccggcacggg ccagccggtg gcgcagccgg cggtacgccg tggtggcggt gagaggcggg
     8821 cgcaggcggc gcgccagcag ccacgcagcg gcggcgagca gcacgaggcc ggccagggcc
     8881 cacatcgcgg tggaggccgg caacggaggc gactccccgg cgccggcctc tccgcccggg
     8941 gccgcgtcgg tggcggcgcc acccgccggc cgctcctcct gctgcttgcg ctggaacatc
     9001 ccccagaagt cggaccagac ctcccgcagg ccggagaaga gccggatctg gtcgtagagg
     9061 ccgaaggtga ggacgtagcg gtcccaccgg aagagcaggt agtcccacgc ctgctgagcc
     9121 agcagccagt tcccctcctg gccggtctcg ggccggccgg ccggcggcgt cgggtcgaag
     9181 gtccgccacc cggtaccggg caggtaggcc tccacccagg cgtgggcgtt gctgtcgcgc
     9241 acgatgtagt agccctcgaa ggggttgtgc tcgccgccca ggaagccggt gaccagccgc
     9301 gccgggatcc cctgggagcg cagcatcagc accatggagg aggcgaagta ctcgcactgg
     9361 ccgctgcggt accggaacag gaactcctcg atcgggttgt cgctcgaccg gccacggaaa
     9421 tccagggtgt agctgtagct ctggacgagg tgcgtctcca gccggtacgc ccgctcgccg
     9481 gccgggccct cgcccatgac ccgggcggcc aggccggaga tgcgcggcgt cacgccggag
     9541 aggtcgagcg tcggctccgg cgccggcccg ggcaccttcc ccagcaggac cggctgcccg
     9601 gccaggccca cccggtactc gatgatctcc accggatcga agctcagcga caccgcgccc
     9661 ccctggtcga tctcgagccg cggcgtgcgc ggctccacga tggcggactg caccggcagc
     9721 ggcaggctgc ggctgcgcat cggctggagc cagatgcgca cccagcggag cggctcctcc
     9781 cggctcagcg ggaagcggat gccctgctcg cgccgcagcg gccccacctg cggcgagcgc
     9841 cgccagctcc cgccctggta gacgtcgtag gtggccgcct tgaagcggac ctcctcgtcc
     9901 ggatcgaact gccccccctc ctggaccagc cgcagggcca cctcgcggct gctgcggatg
     9961 ttgccgatcg agtccagcgt cacctcgtcg gagaagccgg cggcctcgag cgtcgtgccg
    10021 gtgctcatgc cgcggccgac gatgaacggc gcccgcacgc gcggcagcag cgcgaagatc
    10081 ggcaccgcca gcaggaccgc cgccagggcc gacacccaga ggaagccgcg catcgggatg
    10141 cgggcgagcg ccgggtcgtc gcggccgaag ccggagagga cgtggaggaa ggcgaagcgc
    10201 gacagcagca ccagcgacac caccataaag ccgacgaggt acagcaccac cgccggatgg
    10261 acgctggtgc ccatcgccgc caggaagatg aagaagatgc cgatcgccgc ctgccacttg
    10321 tcccgctcgc gctccaggga gaagagcttc accagcaggg cgaacaggca gaggtggacc
    10381 gccggctgca ccagccggcc gcgcgagaag gcggtgaggt cgaggaagaa gatgggcagg
    10441 taggcgaggc ccagcaggtt catcccccac cgcggcagcc agcgcggggc gtcctggccg
    10501 gcgcgccgca ggaagacgag caccccggcc aggtagaccg ccaggaccgg ccagagcacc
    10561 aggtcgttga acggcagcgg gatcggggcg aagaacgcca gccatcccag gaggaggcgc
    10621 ttctggcggc cgaagctcac accgccacct ctttcggctc cggcgctccc tcgggctcca
    10681 tgtgcacgcg gagctgcgcc gcccggtggt cccccggcag gagcacctcg ccggcgcgcg
    10741 gccgcgcctc caccagcgcc agcgactcca gcaccgccag ccgctgccgc gcgccgccgg
    10801 cgaaggggag gtgctggtcg tgggtcacca gctcgacctc gtagccgcgg gccaggtggt
    10861 cgaccgccgc cgtcgcggct tcgctcacca gccgctcgaa ccgcttctgg ctctcgggat
    10921 cggccagctc ccccaccgcg ttgtcgaaca ggatggagag gcgccgggtg cgctcggact
    10981 cccgttccat gtagaccagc tcgccggtgc gcgccgtctg cttccagtgg atgccgcgcg
    11041 gatcgtcgcc gcgccggaac tggcgcaggg agtggaggcc gtggccccat ccggtgcggc
    11101 ggctcgcctc ctcccccatg tggtccacct cgtccagccg gctggcggcg gcggcgaaga
    11161 tctcggggaa gaccagcagc tccaggtcca cacggtagcg cacccccttg cggaagaagc
    11221 cgtaagggaa gaggctcgac acgtgggcaa acggcaggct ctggcggccg cgggacggca
    11281 cgatcatgtc gatgctgccg ctgctcctcc cccgccgggg caggtaaggg atgaggagcg
    11341 gctgggccga gcgcgagacg gtgaacagca ggaaccagcg cgggaagagg cggctgcggt
    11401 tgcgcagcga gaagccgatg gcgaacggcc ggttggcgaa cacctcgccc ggcggctcga
    11461 gctcggcctc gaggccgcgc acgttctccc gcgacatcac gccggagacg atgagcaccg
    11521 cgaacatcac ggccagcacc gtgtagaggg cgttgttgcc ggtgttggtg gcggtgatcc
    11581 ccaccaggat ggagaggagc acgtaccaca gccccaccgt ggtgatgcgg atcccctccg
    11641 gggtcgaggt ccgcttccag ctcatcggcg gctcaccgac cgctcacagg gggaccggcg
    11701 tggcggcgac cacccgctcg atcgccttcc gcgccatctc gaggccggcg ccgccgcgct
    11761 tcagcacgac gcggtggccg aggaccggca ccgccaggcg ctgcacgtcg tcggggacgg
    11821 cataggagcg gccctcgcac agcgccaggg cctgggtcgc ccggtacagg ctctgcacgc
    11881 cgcgggtgga gaccccgacc aggaagtcgc cccccttgcg ggtcgcctcg gcgagcgaca
    11941 gcatgtactc ggccagcttg tcggcgacgc ggacgagccg cacctgctgc tggagcgcca
    12001 gcaggccctc gcgcgagagc accggctgca ggtgctcgag gaggtcctcg acgccgccgg
    12061 acagcagcag ctccttctcg tcggcgcgcg gcggatagcc cagggagatc gacatcagga
    12121 agcggtcgag ctgcgactcg ggcagcggga aggtgccctg atactcgacc gggttctggg
    12181 tggcgaggac gatgaacggc tcgggcagcg tgtagcgctt gcgctcgatc gacaccttgc
    12241 gctcgctcat cgcctcgagc agcgccgact gggtcttcgg cgtcgcgcgg ttgatctcgt
    12301 ccgccagcac gaggttggcg aacaggggcc cgggcacgaa ctcgaacgcc tggttcttct
    12361 ggttgaagat cgagatgccg atgacgtcgg acggcagcag gtcgctggtg aactggatgc
    12421 gctggaaggc gaggcccagg gagcgggcca gggcctgggc cagggtggtc ttgcccacgc
    12481 ccggcacgtc ctccaccagg acgtggccgc gggccagcag gcagacggcg gccatgcgga
    12541 tcgcctccgc cttgccgcgg accacccggg cgacgttgtc ctccagagtt cggatcggct
    12601 ccacggcccc gggtgcattg gccgcatcgg ccgcatcgat gagaatcggc tccatcgcgc
    12661 tctctctaac ctcgcaatgc ccggcgcctc ttccaggcgg gtacagaaga tcccctccgc
    12721 ggctcggaca aggcccggcg gcaggaacgg gggctcatcg tcaggtggct cgccatttta
    12781 atggataggc gattcgccaa caatctcccg gccgcggagc ggcagtctat cgtagggagg
    12841 gctcccggat ttgaccgccc tgtttccgtc gtttactatg ggatcatcga aggctggaag
    12901 gagcaaagga aaccacatgg caacagatct caaggcggac cggttgaagt ccatcgaggg
    12961 ggcgctctcg cagatcgaac ggcagttcgg taagggggcg atcatgcgcc tgggccagcg
    13021 ggaggcgctg gcgatcgagg cgatctcgag cggctccatc acggtcgatc acgccatcgg
    13081 catcggcggg ttcccccgcg ggcgggtggt ggaaatctac gggcccgagt cctcgggcaa
    13141 gacgaccctg gcgctgtcga cggtcgggca ggcccagaag cggggcggca ccgccgcgtt
    13201 catcgacgcc gagcacgcgc tcgacgccga gtacgcgcgc aagctcgggg tcgacatcga
    13261 caacctgctg gtctcccagc ccgacaacgg cgagcaggcg ctggagatcg ccgagatgct
    13321 ggtgaggagc aacgccgtgg acgtggtggt cgtcgactcg gtggccgccc tggtgcccaa
    13381 ggcggagctc gaaggcgaga tgggcgattc ccacgtcggc ctccaggcgc gcctgatgtc
    13441 gcaggcgctg cgcaagctga ccgccatcgt ctcgaagtcg cggacctgcc tggtcttcat
    13501 caaccagatc cgcgagaaga tcggcgtcat gttcggcaac cccgagacca ccaccggcgg
    13561 ccgggcgctc aagttctatg cctcggtgcg ggtggacatc cgccgcatcg cctcgctcaa
    13621 ggacggcgag gaggtgacgg gcgcgcgggc caaggtcaag gtggtcaaga acaaggtggc
    13681 cgcccccttc cgccaggccg agttcgacat cgactacggc gaggggatct cgaagaccgg
    13741 cgagctggtc gacctggggg tcgagcacaa gctgatggtc aagagcggcg cctggtactc
    13801 ctacggcgac ctgcgcgtcg gccagggccg ggacaacgcc aagcagttcc tgcgcgacaa
    13861 ccccgacctc gccgacgaga tcgagggcaa gctgcgggag aagatgggca tgccgcccgt
    13921 cgccgcggtg ccgaaggacg agtagagccc gccgcctgag aggggggccc cgcccggggg
    13981 cctcccggcc gtcttcagga tagaaaatca gcccgcggcc tgggggaaga agcggcgcca
    14041 ccagcgctgg atggggggtt gcccgcgctg gtcggcctct ttctcgatct tctcgagcgg
    14101 ggcggggagg agcgccaggc tccctttctg gagctggagc atcaggaggt cgagctcccg
    14161 gccgcgctgg cgcgggtcgc ccgagaacgc ctcgccgagg gcggtgcggc cgtcgaagcg
    14221 ggcgtagaag tcccgcgccc gcatggggat gcgctgccgc tcgtcggtcg tgggctccag
    14281 cacccagacc ggatagcagc gctccaggag gtccagcggc accgccatgt gacgccgggc
    14341 caccagcacc tgctcctccg tcgcctgctc cagcagcagg tcgaacaccg acagctcgcg
    14401 ggtgccggtc tcgttcgcct cccccacctt ctcgatgtcc agcaccatgg cgacggtgag
    14461 gcggagggcg gcttccagaa accggaagta ggagcccttg agctgctcgt acaggtcggc
    14521 caggttcttc ttgccgtcca ccaggccggc gagccgccgc tccatcggct cgaggccctc
    14581 ccccggccag gcgtcgcccc gccgcagcac cacgttgtcg tggacgaaca cccggcggat
    14641 gcgggcgtgc tcgtccaccc agcgggtgcc ctccatcacc agccccatgg agtggatcgg
    14701 ttcggtgagg atctcctcct cctgcggcag ctcggactgg aagtagaaga cccctccccg
    14761 ccagaggaag aggtcgcaga tggagtcttc gatctgccgc cgcagggtct gctggatgac
    14821 ctcgggcgag agcaggccca gctcacggag cgccaccccg aggcgggtcc gccgggtcgt
    14881 gcagtgagag agcgcctgga agagcgcgtc ctggcccaca tggccgtgga gcagcaggtg
    14941 ctggccgtag aactccgccg ggtcgttcga cagacaggag accacctctc ccagatggaa
    15001 gtagatgcgc ttctcgcgct ggctgcggcg cacgaccagg gcccccgtgc agcgctcgtt
    15061 ctgagcccac tgcaggaggt cgcccatggg gaaggcggcc aatctcccgg agaattccat
    15121 gactgcgggt tcggattgta atccagtcca cagccgaatg gtgtgccccg ggtgcggtat
    15181 gcttctgcgc catgtgtcgc atcctacgcc cgacccttct gctgattgtt tgcatgtcca
    15241 tagcggccgc catggccgcc gcgcccctcc cctgccagcc gtgcgccggt gtccggctcg
    15301 agccgctgca gccccaggcc gcccccgccg ccccgtctcc cgccgagacc ggcggggtgt
    15361 cccaggggtc ccagccggtc cagatcccgg aaggcaatcc caccgagatc cagccgcagc
    15421 agctcccgcc gatggtggcg gggccggact ccctccccac cgtcccctcc gagatcgggg
    15481 cgctgctcca gcagagcccg aagctcgagc cggggagccc gctgttcgtc gcctgggacg
    15541 tgctcctggg agaggaggcc gctcccgtgt cggtgggcgc cgaggcgctg ggcatcgtcc
    15601 agaccggggg caccccctgg ctgtcgctgg tcttccgcac cccggccccg gtgacgcaga
    15661 acgtcgcccg cctccaggcg gagctgcgcg gcgccgccgc catcgccgcc gccgcgcccg
    15721 ccaacacctg gttccaggtg gcctggcgcc cggagggggc ggagacccag gcgttcgcgc
    15781 ccggggagta cgccttcctg ctcaagcgcg ccgccgtggc gctgaccggc gcgcgcgccg
    15841 acgccaaggt ggcctcggcg ccgctgccgg ccgacccggc gcagatccgc gccttctatg
    15901 gggaagaggt cgcggcctac ctggaggccg tggccctcca gcccgcgccc gccgcggcgc
    15961 tgaccgccgc cgtggccgcc ctgcaggagc tcgatcccgg ccgcccggtg gtgctggacg
    16021 ccgtcgacta ccccgccgat ccgtccggcg tcctctcgga ggccgcccgc cagtcggcgc
    16081 gggggatcgg cctgacgctg ttccgcaagg ccaagctgac cgccgccgac ctggcccccc
    16141 tggcgctcct cgcccgcgag ctcgccggtg acttcaccta cgacccggcg tcgaccccca
    16201 ccggcgccgg cgaggcctgg acgttcatcg gcgccgccag tgatgccggc ggtgcaggcg
    16261 acaccctgga gctgcgggtg atcgccgagg cgccggccgg ggccagcgag ctgaccctcc
    16321 gcttccccga cgcctacctg cgccgcccca cgcgcttccc ctacacgccg ggccgcttcg
    16381 acccgccgag cggccgggtg gagggggacg cccttctcct caccgtgacc aacccgggcc
    16441 gggtggaggt cgtgggcctc gagcgcgcca ccgccgccga gcgcgagggg gtcgaggaga
    16501 gggtgaccat cgccaccgag cgcgagatgc cggtcgagga gatcctgcgc cggctccagg
    16561 cgttcgagga cgcccaggcg cggcggctcg accattaccg cgccaccaac accacccata
    16621 tgcgcttcca gcccgcggcc ggcacccaga gcatcgaggc gacgctccag ggccccttct
    16681 tcttcgatcc acagaccggc tccgactggg cctgggaggc gctctacgtc aacggcgtgc
    16741 gctggcgcgg caagtcgatc cccgagatcc cgctggtgca gccggagaag gcggcggcgc
    16801 tgccgctgga gatccacttc aacaagcagt accggtaccg gctgcgcggc accgacaccc
    16861 tcgacggccg tcccgcctgg gtggtggact tcgccccgac cggcccggcg gccgaggggg
    16921 gcaagctcta ccagggcacc gtctgggtgg accgccagat ctactcgcgg ctgcggaccc
    16981 gggcggtgca gctcggtctc gagggggagg tcatctccaa cgaggagacc atgcactaca
    17041 cgccggtgga cgccgaaggc aagcccgcgc cctggggccc gcagagctac gtgctgccgc
    17101 tgcgcatcgt cgcccagcag ctcctgtcgg tggtcaactc ggcgaccgtg gtggagcgcg
    17161 agaccctgct caccgacgtg cggatcaacg ccgccggctt cgaggaggcg cggcggcagg
    17221 tggcgggcac cgacgtcacc atggtgcgcg acaccgccga gggcctccgc tacctggtca
    17281 aggacgactc cggtgagcgc gtggtgcagg agggcttcga cacctcgaag ctcttcgccc
    17341 tgggcggcgt cttctatgac gacgcgttgg actatccgct gccgctcgcg ggcgtcaact
    17401 acttctcctt cgacttccgg gggacgggca agcaggtcaa cgccttcttc gccggcgccc
    17461 tgctgacggc ggacatcgcc gagccgcgcc tcttcggcag ccgcttcgac gccggcgtgg
    17521 acctcttcgc cctggcgatc cccctcaccg acaccgtcta ccgcggcgac cgggaggcca
    17581 atgaagagga cgtggagctg cgcaccggca acatcggctt caagatcggc catccggtcg
    17641 gcaacttcat gaagctcggt gccgagtaca ccccgctctt cttcaactac ggccgggcgg
    17701 acgacaccga cccgaatttc gtcatccccg aggaccacgt cctccacgcc ctggacctgc
    17761 gcgcctcctt caaccgctcg ggctacggct tctcgctgac tggcagctac aacaagcggt
    17821 ccgagtggaa gccctggggc ctgccgggca atcccgagta cagcgaggac cacgaggact
    17881 tcctgcgctg ggaggcgcgg gcgagcaaga actggtacct gcgctctttt caaaaggtcg
    17941 ggatcgagtt cgactacgcc tcgggccagg atctggaccg cttcagcaag tatcagttcg
    18001 gcttcttcgg cggcacgcgg gtgcacggct atcagagcaa ccgcgtgcgg gcgacggagg
    18061 tcctggcgag ccacgtctcc tacggcttcg aggttggcca gatgctccgc ctcgaagcgc
    18121 tgggcgacgt cgcctgggcc accgatgagg acaccggcct ggacaacgag ctcctggggg
    18181 gcctcggcat cgccggcacc ttcatgggcc cctggcagac cgtcgtcaac ctcgacctcg
    18241 gcgtcccggt ggcgggcccg gacgacggct tcgtggtcta cgtggtgttc ctgaagctgt
    18301 tcaactaggg ggacggcatg gaagacgaaa aggtatggga ggatcccgcg gagccggtcg
    18361 agctcccgat cacggacatc ctggacctcc acagcttccg gccgtccgag gtggccgacg
    18421 tggtccgcga gtatctggac gcggcctacg agaagggcct gcgggacgtc cgcatcatcc
    18481 acggcaaggg gatcggcgtg caacgccaga ccgtgcgcac gatcctcggc cgtgatccca
    18541 gagtcgaagc ctgtggcgac ctgccgggag aggcgggcgg ctggggggcg acgtgggtgc
    18601 ggatgcgcta gcccttcagc tctttcctga agtacacgac ccgctcggtc tcttcgaagc
    18661 ccaacgcccg gtgaacggcg tggctcagct cattgtcgag ataagcgtcc gacgccagct
    18721 cgctgcaccc catctcccat ccccaggcct cgaccgcccc aaccatctcc cgggcgactc
    18781 cctggccccg tgcctcgggc acgacgtaca tcccctccag gaaggcgacg ggagagcttt
    18841 ccgttccgtt cacataatcg gtccgcaccg aagcctcgac aaatcccaga ggctcgcggt
    18901 caggtccgta cgccacgacc tgcacgtatc tcccgggttg ttcgaggaac gaagccatct
    18961 cctcgcgatg ctgctccggc gtgccctcgg gccagagggc catccgcagc tcgagccagc
    19021 cgggctgttc gacggaggtg cagtgttcga tcatgacgcg ttctcctgat ctgctccggt
    19081 cagggggcca gaaaatccac cccatagtcc caggcctcct cccaatcgcg ccgcgcctcg
    19141 gcccagcgat tcagcgcgtg agcgatgcgc tcaggctgac tgttcgggag actccgaggg
    19201 ataacgagca gtcccgggct cggctccccg tcctcataga aggtcacggt caggtggacg
    19261 aagtcatccc gattccgagt tacgaagatg cggccttggg ctgccgagaa ccggaactga
    19321 tccgggtccg tgaaacctcg ccgggagatc tcgtggacac tgacggcatc gagaccgagg
    19381 ccgcgcgcga tctcggccac cttgggattg acgtcctcgt cgaggaggaa ccgcatcaac
    19441 cttgccgcgg ccccttttgc ttcgcccggg ccagctcact cgagaccttg gcgggcgtcc
    19501 actcctcctc gcgggccaga cgggcatcga tctctccggg gtacagctcg tagtaggtca
    19561 atgccgcccg gagctgaggc tcggtgagcc agggatactc ctggcgcagg cgtccgaggt
    19621 cctggccgac ctggcgccag gtggccacca cctcccagac gtcgaggccg ctcccagcca
    19681 ccccggcccg ccggcccgcc ggcccctccg agaacgagat cccggggacc cggcgcatgc
    19741 ggatcgcctc ttcgagcagc tccaccgtcg tcgcggacca cgacttgcct ctctgttcgg
    19801 cctcgcgctc gatctcccgc tccaggttgg acggaacttg aatggctctc actggctctg
    19861 ccacggctct agcctcccgg ttcagtctgc ctctctaccc taccatcccg caagggcacc
    19921 ggcctcgccc cgccacctcc ccttcgagcg cggctcccgg tgggcgtagg cgtatcccct
    19981 tttgctaagc tggtcccgtg gactccccat ggcggtcttt caagaagctg ctgcggcgcg
    20041 gacggctccg gctcgcgcct catgccggct ggccgcgggt cgatttcgtc tatggcaggg
    20101 agtaccagac ggacctgccg gcggtctctc atgactcgcg gcgcggcgaa cggatcctgt
    20161 cgttcctcga ttgggagggg ctgctcgacc gccgcttcct gcaccacccc gagcccgcca
    20221 cgtaccggct gctgcgccgc gtccatactg acgagtacct cgagtcgctc aaccagaccg
    20281 ccaccctcac ccccattgtc gggttcgaca tggccgaacg cgcggcggag cgcctcgtcg
    20341 aggggcagcg cgccatggcc ggaggcacgg tgctcgccgc gcggctggcg ctggagtcca
    20401 aggggatcgc ggccaacctg gggggaggct tccatcacgc gttcgccggc aagggggcgc
    20461 ggttctgtct gttcaacgac gtggccgccg cgatcaccga cctgcgcgac aacggcttcg
    20521 gcggccccat cctgaccgtg gacctcgacc tccacgacgg cgacggcacc cggtccctct
    20581 tcgcccggga cccgacggtc tacaccttct cgatccacaa ccagtccaac ccccaaccgg
    20641 gcgaggaggc ggtggcctcg accgccatcg agctgggcgg cggcgtggac gacaagacct
    20701 acctcgacgc catccgcgcc cacctgccgc ccgtcttcgc cagcttccgt cccgagctgg
    20761 tgatctacct ggcgggctgc gacccggcgg cgggggacga gatcggcgac tggaagatct
    20821 cgtcccaggg catgctggag cgcgaccgct tcgtcctcca cctgagccgc ggcctcggcc
    20881 gcagcgggcc gcgccggctg ccgctggtca tcgtgctcgc cggcggctat ggccagcgct
    20941 cctggcgcta cagcgcccgc ttcctctcgt acctgctcct cggccgcccg gtggaaccgc
    21001 cgaccaccga cgagagcgtg gtgatgctct accggcagct cgcccgcggc tacggcgagc
    21061 ccgagctcac cagcgagccg gccgcctccg aggatggggc ggattggggg ctgaccgacg
    21121 aggacatcgg catggccctg ggagggccgc ggcgcccccg gcgcctcctc ggctactaca
    21181 ctccccaggg gctggagctc gccctggagc gcaccggctt cctcgaccgc ctgcgcgccc
    21241 tggggttccc gcggcccgtc gccgagctcg acctcgacaa cccgaccggc gacacggtgc
    21301 ggttctgggc cgaccggcgc aaggccgacc tcctcatcga ggtccgcatg cgcgtcgacc
    21361 gcggcaccgc tccggacctc gccctgctgc gcgccgagtg gctcctcctc cagaaccccc
    21421 acgcccagcc cacccccgaa cgccccctgc tgccggggca acgccatccg ggactcggca
    21481 tgctccagga cgtcatggcc ctgatggtgc tggtctgcga ccggctgcag ctcgacggcg
    21541 tgctgttcgt ccccgcccac taccataccg ccgcctacgg ccggaagctg atgcgctcgc
    21601 tcgaccccgt ggacgaggcc cgcctgcgcg cgttgcgggc cgtcctcgac cgcctccccc
    21661 tgtcggaggc gacgcacgcg atggagtcgg gccgggtgat cgacacccac accggcgatc
    21721 cggcggtctg gcagcccagg cccctggtct accccgtgag ccagcggctg cgcgagaggc
    21781 tggaggcggg cgactacgag cgccaggtgg ccgaggcggc gccgcggttc ggcttcgcgt
    21841 tgcgtcaggc cgccaacggc tgaaccagca ggatcagggc ggccagggcc tcgcgctgca
    21901 cccctcggga ccggaagatg gggaacgcct cgaccgccag cctacgggcc accaccggcc
    21961 gacccagccg ggcatggagc gccgccagct ccagcgagac cagggcggcc tcgtagccgt
    22021 tccccaactg cgcgaacccg ctccgcaccc ggtccagtag aaggacggcg cgggggagct
    22081 gccccaggcc tgccaggatc tgagcctcga gccaacgcat gcggagctgg atcatcgaat
    22141 cggagacctg gcgataaagg gcgcggctgc ggacaaggag ggcgcgcgcc tcccgcaggc
    22201 gtcccaggtc catgaggctg accaggaggt tgtggcggat ggcgatcttg aggcgcgggt
    22261 ccaggtccgg gccggccagg ccgttcgcca ctcgcagcag gcgcaccgcc tcctccggct
    22321 ctcccgtctc ctgacagacc ctgacccaaa ccaggaggct cgtcaggact cgccggaggt
    22381 cccccgcgct cagcgagatg gagatggagc gcttgaggag gctgatggcc tcgccgaacc
    22441 ggcgctgcgc ccggcgcagg gacgccttgt aggccaggac cggagcccgc tgcagcgggt
    22501 cccgcgatcc ctggcgcagg tgggcctcgg ccgcccggaa ggcctggtcg gcgccgagga
    22561 ggtccgtggc cacccggcgg gcgttgccga tcgaggccca gcagcgggcc cgggcgtctt
    22621 cgaccacccg cgtcccgtgg cgcccgggat cgaggcggtc ggcgagccgc agggccagct
    22681 cgccccaccg ctccccctcc ccggcgtcgc ggtggacctc ctcgtcgctc gtcctcacca
    22741 gcagctcgac gagcgccagg gtgcggaagc gcgtcctctc cgcgatcagc caggcgcggc
    22801 gctccggtgg atggcgtctc agctcctcca ggaggcccgg cgccgcggcg cgctcccgct
    22861 ccacggagtg gaaatgcgac tggaagccgc gcagcacccg gtcgacgacg atttcatact
    22921 ccgtctccgg agggcgccaa gcgaggaccc gggcacccac gggctccccg cccccctccc
    22981 cttcctccgg ccccagccac agctcgtggc agcgagggca ttcgcacaga tgcagcagga
    23041 cacggttccg gctctccggc accagctcaa gcagacggtc gatgacctca ggcttcgggt
    23101 gaatcctcgg catacctttc tggggacctc tggagtatca gttgccgcag acgccgtttc
    23161 agagacaaag agacatatga tctcttcgag cctcttatga ccgagagcgt cagcctcccg
    23221 ctctggctgg tcctgctgct cgtcctgctc gcgggatggg cggtcctgga gcgcctgctg
    23281 gtccccagcg tccgctggta tctgcggcgc cgggtcaacc gtgtcatcga ggagctgaac
    23341 acccgcctgc gcgtcgagat ccgcccgttc cagcgcaccc ggcgccaggt gctgatcgac
    23401 cggctgatct acgatcccaa ggtggtcgag gcggtcggcg cccgcgcccg cgagcagggg
    23461 atgccccgcg acgtggtcat ggccgaggtg gagcgctacg cgcgggagat cgtccctgcc
    23521 ttcaacgcct acttctactt ccgcatcggc tactggatct cgcgggcggt gacgcggctc
    23581 ctctaccgcg tgcggctcgg cttctccgac gaggccggcc tcgccgaggt cgccccccac
    23641 tcggctatcg tcttcctgat gaaccaccgc agcaacatgg attacgtgct ggtctcctac
    23701 ctcgccgccg accgcaccgc cctctcctac gcggtgggcg aatgggcgcg catctggccg
    23761 ctccagacgc tgatccgcgc caccggcgcc tatttcgtgc ggcgcggctc gggcaacccc
    23821 ctctaccggc gggtgctgga acgctacatc cacatggcca ccgccgccgg cgtcacccaa
    23881 gccgtctatc ccgagggggg tctgtcgcgc gacggccacc tgcggccgcc gaagctgggc
    23941 atcctcgact acatgctgcg cggcttcgat ccgcaggggg agcgcgactt ggtgttcatc
    24001 cccgtcggga tcaactacga ccgggtgctg gaggaccgca cccttctctt ggatgtgcag
    24061 ccgccgccgg aggaccgcaa gggccccctc gccgccgccg cgaccgcgtc cggcttcgcc
    24121 ctgcgccacc tcgggctgat gctggccggc cgctggcacc ggttcggcta cgcctgcgtc
    24181 aacttcggca ctccgctctc catgcggcgg tggctcggcg agcgccggct cgacctgcga
    24241 atcgtatcca gggaggagcg ctcggccgcc ctggaagagc tgggcggcga gctgatggag
    24301 cgggtgggct ccgtggtgcc ggcgctgccg gtgccgctgg tggcgagcgt cttcgtccag
    24361 cgcctcgacc gtctcgacgg ggagatgggc gggccgctct ccgagctgga gctcaaggcc
    24421 cgggtctacc ggctgatggg cgagctcgaa gccggcggcg cccacgtcta catcccccgg
    24481 agcgaccagg actacgccat ctccgtgggg ctcaggaccc tcatccagcg ccacctcgtc
    24541 gacgagaggg gcggcctctt cgcgcctcgc gagacggaga tcgccgtgct ccgctactat
    24601 gccaattcga tcgcgcacct gattccggaa acacccgcgc agccggcacg tattacaagc
    24661 tgaagtgaga gcctcggtca ccccgccccc actgcgccgg ttcgaaaggt tcgtccgcga
    24721 ctatacggac ggcctcaaca cccgcgacct gcagcgcctc ttccaggggg acgccgcgcg
    24781 ggcgtacgcc gtcctcaccc gcgagcacgg acagcagccg gagcccaagg acggattgcg
    24841 gcggctcctc caccggatca gactctcctt cctcggcctc tcctacaagc tgagcccggc
    24901 gcgccgtttc ctcttcgccc tgtcggtcct cttcttcctc atgggctgct tcttcgacgg
    24961 ggcgatccag gtcgacaccg gcacgacccg gtacgcggtc ggcttctccc ccgtctactt
    25021 ctcggtgagc ttcggcctgc tggtctacct gctggcgctg gagctggtgg accgggtgcg
    25081 ggtgcgtgac gagctgcagg tcgcccgcga gctccaggcg gacctgctgc cgcgcgaggt
    25141 gccgccggtc ccaggctact ccttcgccca ctcctaccgc acggccaacg agatcggcgg
    25201 cgactactac gacttcacgc cgctgcccga cggccgcgtc gccatcacca tcggcgacgc
    25261 cagcggccac ggcatggcgg ccgggctggt catggccatc gccaacgcca cgctggagac
    25321 ggcgctcgac ctcgatccca ctccggagcg ggtggtgtcg ctgctcaacc gcaccctctg
    25381 ccgcaccggc agccggcgga ccttcatgag cctgttcttc gccctgctcg accccgccac
    25441 cggccgtctg gactacgtct gcgccggcca ccccttcccg ctgcttcggc gggtgggcgg
    25501 cgcgatcgag gagctcggct gcggcggctt ccccctgggc atccgggagc cggcggggcc
    25561 ggtctgtaag accacctttc tggagccggg ggacctgctg gtgctctaca ccgacggcct
    25621 cgccgaagcg gtgaaccccc agggcacgga ggcgttcagc ttcgaccgcg tccgcagcct
    25681 gacctcagtg ggcggctcgg cccgggacgt ccacgactgg atcctcaagg ctttcgaccg
    25741 ccacgtcgcc ggcgagccgc tgaaggacga cctcaccctg gtcgtcctcc accgctcgcc
    25801 gtttcccgtc ccgccgccgg tccccctctg aagcaggaag ggccgcctcc tggcggagac
    25861 ggcccctggt gtccaacgga tgaaggctta cggctggaag ccgaagcggt cgttgatgcc
    25921 gttcagctca ctgctgtccg gcccgacgaa cggcaggtcg tagtacttga tgtcgccgga
    25981 gctgagctgc tccacccaga cctcgacccg cgcgttggtg aagcggatga cgatcggcca
    26041 caggtagccg ttcgggcgcg ggccgatgac ccgcagcagc acctcggggc ggtcggggaa
    26101 gacgccggcg gcgcagaccg tctgctcgat gcagtcggcc acctgcgttg ccatgcgcga
    26161 gacgccgccg gtggtcaggc ggaccttgaa tcggaaatcg ggcatgtcgc tgctgctcag
    26221 ccaggggccg gccggcggcg tcaccggtcc gaaggaggtg ttgccgctca cggtgccgtc
    26281 gtggccgccg atgatgtcgt tgccgttgcc gtcgagcgac cagaccgcca ccagacccgt
    26341 ggtcggggtg gtgatcggcg tgcgcatcgt gctctcgatc tgggcggccg tgcgggccac
    26401 gttccacagg cggacctcgt cgatcgagcc gttcgggcta aagaaatagg aggcgtcgct
    26461 gccgatacgg acgttgtcgg tgctcgtggt cagcggcgcg ctctcggcga acgcgcccac
    26521 ctgcacgccg ttgacgtagt gcagacgggt ggtgccgttg aacacgaccg cgatatgcgt
    26581 ccaggcgttg tcgaggatcg tgccgccgtc acggacggag ccggagccct tgagatagga
    26641 gcgcagcgtc gagccgcaga cgccgatcca gtaggcctcg gtgtagtcct tgccgaagag
    26701 gctcacgcaa cccggctggg gcgtgtcgag cttcacccac atctcgatgg tcagcgccga
    26761 cgtcgggttc aaggccgggc tgtggggaat ctcgatgtag ccatgagtcg gagggcccga
    26821 cgtgccgtcg ccggacagat cgaggtatcc cccgaagggc tgggcctgag ctgggggtgc
    26881 caggacgacg gccagcaggg ccagggtgag ccagggcaac caatgatgcg acgactgccg
    26941 gacgagcttt ccattcacta gcatccatct tctcctgtga gtgggtactg gttgacgctc
    27001 cggagtcttt gcaacttccg tgcatgtctc cgacgggccg ccggaagctt ccaacgcgct
    27061 ggcggaggtt acaacgtgcc gttgcagtct tcgtcaggcc cttggaggct tccaacactc
    27121 tggcgaacgt tgcaacgcaa cgctgcagtc ttcggcgagc cgttggaaga ttccaatgcg
    27181 ctggcgcacg ttgcaacgcc ccgttgcagt cttccgcgag ccgttggaag cttccatcgc
    27241 gctagcgcag gttgcaacgc gccgttgcag tcttcgccga gctggcgaac gttacaacga
    27301 gcgattggaa gcttccagcg cgccgtcgga gactacgacg cgccgccgca agctccacca
    27361 agagaaaagc gtctcccccg cgacgacgga gggtcctgta ctccaacgct ggctgggccg
    27421 cgcctacgcc gtcagcgctg gggccgagcg gccccggtag aacgcgaggt aggcctccag
    27481 cgtctccaag gcgaggcggt cggcggggct gccgacgacg cggatgccgc cgtggcgcag
    27541 gtggcgggcg aggcgccgat tggcgagcac gatctccagg ctcgccgcct gggcgaacgc
    27601 ctgctcgggg gtcgccggct cgtcgtcggc cagggcgcgc agctcggggt cctccaggtt
    27661 caccagcagc gagcggtggc ggcgccccag gcgcaggagg gcctcccgca gcagctcggc
    27721 ggccgccagg tccaccaccg aagtgaagag gacggcggtc gccccccgcg gctcgacacc
    27781 gaaaaccgcc tccaccccga g
//
LOCUS       DASPJI010000655         3486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_389019, whole genome shotgun sequence.
ACCESSION   DASPJI010000655 DASPJI010000000
VERSION     DASPJI010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389019"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VEL74_18555"
     CDS             complement(<1..795)
                     /locus_tag="VEL74_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64588.1"
                     /translation="MEKKTFRKRLGIVWALFVVLAVGAPSSPAHAQDLKKLATDLAMQ
                     IHALDHNRVTVVDFLDLDGKPNKLGKLLAQDLQEALADPKLKLQVVDQGHLPELFEQI
                     KKADEGLLDPATSKALGKIAGTEVMVVGTVMPSSVTIKLQVKAIDLETAKLIKAQSAS
                     LARVGLRGALATESEGGGAADEAESAKEEVVAKAAKPKVQAPARVLQDQGVIFELDAC
                     SLSGNALTCELTVTSERRDRRITVTHRSRAWSDSGDEYGPNEITIAN"
     gene            complement(978..1520)
                     /locus_tag="VEL74_18560"
     CDS             complement(978..1520)
                     /locus_tag="VEL74_18560"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG64589.1"
                     /translation="MLPELQTERLTLRPAAESDLDALWALWTDPAVRRFLFDDVAVSR
                     ERAAEVLADCRAQMADGLGLWVILTRDRQRVIGCAGLMPVGMAVQYEPRLAGEVEPLV
                     ALDPALWHQGFATEALAAAIGYAFGPRDLPRLAGVTDIPNEASHRLLLRLGFEVLGEC
                     DGPTHRMRTYRLMATSRPQH"
     gene            complement(1530..2195)
                     /locus_tag="VEL74_18565"
     CDS             complement(1530..2195)
                     /locus_tag="VEL74_18565"
                     /inference="COORDINATES: protein motif:HMM:NF044878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64590.1"
                     /translation="MPDIDELFMEHLGASLSKQRSLLQAVEGLPCDIDLDTGEATFGD
                     RYTFPVQLLGFEEEDSQTWIWSWALEEPLGPESLTESALALKEYGEKHGLDLFTVPSF
                     GTEDLSGDEVTILSCGVSGSQSFFAAHTEENEVTFFLIPDTRGQLPTERPAGFVAEVI
                     EEMLDTYELDEPAEVLRSYLRYEGYGLDEQSSRKWVASHPKGGRLTLKFDGQGQLDAV
                     DAG"
     gene            2614..>3486
                     /locus_tag="VEL74_18570"
     CDS             2614..>3486
                     /locus_tag="VEL74_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64591.1"
                     /translation="MTQPSSPPKIRRKTRELTLEQELGRIFDRLAAGTTPAGRQGLQL
                     RLGLTGTPPASLAAAGSAVGLTRERVRQLESRFIESLQQLHPPTPVLDEALRVAEEIL
                     PATDLEIERALAERGLTKEGFSFRSLLKAAKLLGRPTSFSQDSAKDVIYRAGSELPRR
                     FVLSQARGLTLRWGASTFDVLVAELARVRSGVPDPGIVRLHLDTMPSFEWLDDEHEWF
                     WVKNAPPSNRLLVQIEKVMAVAGSIEIGDLRAGIARHPRMEGFRPPREALARLCVQSD
                     RYRREGDRILLGDNH"
BASE COUNT          591 a         1186 c         1134 g          575 t
ORIGIN      
        1 gttggcaatg gtgatctcat tgggaccgta ctcatcccca ctgtcgctcc aggcgcgaga
       61 tcgatgcgtt accgtaatcc gccggtctcg ccgctcactt gtcacggtca gttcacaggt
      121 cagcgcgttt ccactcagcg aacaagcatc gagctcgaat atcacgcctt gatcttgaag
      181 gacacgagcc ggtgcctgga cttttggctt cgcggctttc gcgacgacct cttctttggc
      241 gctctccgct tcgtcggccg cccctccccc ctcggactcc gtcgctagag ccccacgcaa
      301 tccgacgcgg gcgaggctgg cgctctgcgc tttgatcagc ttcgcggtct ccagatcgat
      361 cgctttaacc tgcagcttga tcgtcaccga cgagggcatg acggtgccca cgaccatcac
      421 ctccgtacca gcgatcttgc caagcgcttt gctggtcgca ggatcgagaa gtccctcgtc
      481 tgccttcttg atctgctcga acagctccgg gagatgcccc tgatcgacga cctggagctt
      541 gagtttgggg tcggcgaggg cctcctgcaa atcctgggcg agcaactttc ccaatttatt
      601 gggttttccg tccaggtcga ggaagtcgac gacggtgacc cggttgtggt ccaaggcatg
      661 aatctgcatt gccaagtccg tggccagctt cttgaggtcc tgtgcgtgag caggggacga
      721 gggcgcgccg acggccagaa ctacaaacag tgcccacacg atgcccagcc tcttgcgaaa
      781 ggttttcttc tccatccttt tctccttctc gttcaaaggt cacccacacg gcgcgcacca
      841 cgcccgaaac gagcgaatca tcctgattgg tgattccgtg ggacgaagat ccgcggtgaa
      901 cgcgtccaag agaaagagac acaagcggcg aagaaaaagt ttagaagatc cctgccgccg
      961 caggcaggat ctgaatttta atgttgaggt cgagacgtag ccatgagccg ataggtccgc
     1021 atccgatgcg tgggaccgtc acactcgccc aagacctcga agcccaggcg caggaggagc
     1081 cggtgcgagg cctcgttggg gatatcggtc accccagcca gccggggaag atcacggggg
     1141 ccgaacgcgt agccgatggc cgccgccagg gcttcagtgg cgaagccctg gtgccacaac
     1201 gcgggatcga gcgccacgag cggctccacc tctccggcaa ggcgcggctc gtactggacg
     1261 gccatgccca ccggcatgag ccccgcacac ccgatcaccc gctggcggtc cctggtgagg
     1321 atgacccaga gccccaggcc gtccgccatc tgcgcccggc aatcggccaa aacctcggcc
     1381 gcccgctccc gggagaccgc cacgtcatcg aagaggaatc ggcggacggc cgggtccgtc
     1441 cagagcgccc agagggcgtc gaggtcggac tcggccgcgg gacggagagt cagtcgttca
     1501 gtctggagct cgggcagcat ccggaggagc tatccggcgt ccaccgcatc gagctggccc
     1561 tggccgtcga acttgagggt gagccggccg cccttggggt ggctggccac ccacttgcgc
     1621 gaggactgct cgtccaggcc gtacccctcg tagcgcaggt aggatcgcag gacctccgcg
     1681 ggctcgtcca gctcgtaggt gtccagcatc tcctcgatga cctcggcgac gaagccggcc
     1741 ggcctctcgg tcgggagctg gccgcgcgtg tccgggatca ggaagaaggt gacctcgttc
     1801 tcctccgtgt gggcggcgaa gaaggactgg ctgcccgaga ccccgcagga gaggatcgtc
     1861 acctcgtcgc cgctcaggtc ctcggtgccg aagctcggga ccgtgaagag gtccaggccg
     1921 tgcttctcgc catactcctt gagggcgagg gcggactcgg tcagggactc ggggcccagc
     1981 ggctcctcga gcgcccagct ccagatccag gtctgggagt cctcctcctc gaagccgagg
     2041 agctggacgg ggaaggtgta gcggtcgccg aaggtggcct cgccggtgtc gagatcgatg
     2101 tcgcagggga gaccctctac ggcctggagc aaggaccgtt gcttggagag ggacgcgccg
     2161 agatgctcca tgaagagttc gtcgatatcg ggcatggcgc gcattctatc ctgaccggaa
     2221 ccggccgtcc tgaagaccgc ttcatccctc cccgggcgcg gcctggcctt gccggcgcgg
     2281 cgcggcgccg agcccacggc gggccgcgcc cggccgacaa caggccgcac ctggccgaca
     2341 acaggccgcg cccggcccac aacaggccgc gccaagccga caacaggccg cacccggccg
     2401 accgcaggcc gcgcccggcc cacggcgggc cgcgcccggc cgacgacgga ccgctccaag
     2461 ctcacggcgc gcttcacctt gccgattgcc gacgccgtcc ggcgccggcc aggacgtccc
     2521 aatcacttct cgggcataat agtagcccct ttgaactcgc tgcatgatgc cggaggatgc
     2581 ctcctgacgt ggctctcctc cagaaaggac cggatgacgc aaccctcgag ccctcccaag
     2641 attcggagga agacgcgaga gctgacgctg gagcaggagc tgggacggat cttcgaccgg
     2701 ctggcggcag gcacgacccc cgccggccgt cagggcttgc agctccgcct gggcctgacc
     2761 ggcactccac ccgcttcgct cgccgccgcc ggcagtgccg tgggcctgac ccgggagcgt
     2821 gtccgccagc tcgagagccg cttcatcgag agcctccagc agctccaccc tccaacgccg
     2881 gtcctcgacg aagcgctacg agtcgccgag gagatcctcc ctgccaccga tctggagatc
     2941 gagcgggcgc tggccgagag aggcctcacg aaggaggggt tctcctttcg ctctctcttg
     3001 aaggcggcga agctgctggg ccggccgacg tccttctctc aggactcggc gaaggacgtc
     3061 atctaccggg ccggctcgga gctgccgcgg cgcttcgtcc tgtcacaggc ccgggggctc
     3121 accctgcgct ggggggcctc gacgttcgac gtcctggtcg ccgagctggc cagggtgcga
     3181 tcgggcgttc cggatcccgg gatcgtgcgc ctgcatctcg acacgatgcc gagcttcgaa
     3241 tggctcgacg acgaacacga gtggttctgg gtgaagaacg cgcctcccag caaccgcctc
     3301 ctggtccaga tcgagaaggt catggccgtc gccggctcga tcgagatcgg ggatctccgg
     3361 gctggaatcg cgcgtcaccc gcgcatggag ggcttccggc cgccccgcga ggcgttggcc
     3421 cggctgtgcg tgcagtcgga ccggtaccgg cgtgaggggg acaggatcct gctgggagac
     3481 aatcac
//
LOCUS       DASPJI010000656         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_212119, whole genome shotgun sequence.
ACCESSION   DASPJI010000656 DASPJI010000000
VERSION     DASPJI010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212119"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VEL74_18575"
     CDS             complement(<1..498)
                     /locus_tag="VEL74_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64592.1"
                     /translation="MRVRFLPSWLLTLSLVLCLGACTFEDATEESEDQQAATPSQPAA
                     DPNVISVPSRDYRAYDRAALESGRRDPAWRTYAERDLQERRGGTAAAPQATPGAAGTE
                     PPLTSDFQAETVPPAGAGPEGGAPRPGARAAGQETFDQISPAALQGRITLPVPREEGG
                     PSALRV"
     gene            complement(588..>2481)
                     /locus_tag="VEL74_18580"
     CDS             complement(588..>2481)
                     /locus_tag="VEL74_18580"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYG64593.1"
                     /translation="GALADAVVMESGETALGLAKALAGRSAAILIERRTPDPEVAPMV
                     DDPAVVLSLAEALGLPEELSASLPPAFLVRTPGDAERLARRHPGISFLSRDGVWVEAG
                     TYHVEGDTATPGVLERESELANLARSIPEIEAELKEAIAELSRLVNDRANLAKESNRL
                     QGEVAQLRQELAVAKARMEDAATRHRRLAAEQETLSSEQQEILREIERVAARRGQLQE
                     ELAAAEERNVALQEAFERAQAELEAAKAHRESLRTESAGRRGRLELLDERLESHDREE
                     ARLAAEVERGQRQVATWREEAEKLTGRRGQIEGELEKAQADLQRALEQRAAAEEGVLE
                     EQERLDLHRQEIKDLEERIQLHRDRRDQVRSQAEELRIQQASVKQDAEHLALTFQEQF
                     SRPLPEQPGEAPEGLAEMEAELARCKATLERLGPVNILAVQEHDEQEERFQFLTTQRA
                     DVANSVESLKNTIREINQASSERFNTTFVEVNKSFGETFTRLFRGGEAEMRLLDENDP
                     LESGIEILARPPGKRLQNLMLMSGGEKALTAIALLFALFRTKPSPFCILDEVDAPLDD
                     VNTVRFVDVLKEMAIDTQFIVITHNKLTMEATSTLYGVTMEERGVSKLVSVEIDDVQP
                     EERRTA"
BASE COUNT          300 a          857 c          919 g          405 t
ORIGIN      
        1 gacccgcagg gcgctggggc cgccttcctc ccgcggcacc ggcagggtga tgcgcccctg
       61 gagagcggcg ggggagatct gatcgaaggt ctcctggccc gcggcccggg cgcccgggcg
      121 aggcgccccg ccttccggac cggcgcccgc cggtggcacg gtctccgcct ggaagtcgct
      181 tgtcagcggt ggctcggtcc ccgccgcacc cggcgtggcc tgcggcgcgg cggccgtgcc
      241 gcccctccgc tcctgcaggt cgcgttcggc ataggtccgc caggcagggt cgcggcgccc
      301 gctctccagc gccgcccggt cataggcccg gtagtcgcgc gagggaacgg agatgacgtt
      361 ggggtccgcg gcgggctggc tgggagtcgc cgcctgctgg tcttcggact cttccgtagc
      421 gtcttcgaac gtgcaggccc ccaggcagag caccagggac aaggtcaaca gccacgaggg
      481 caggaatcgt acgcgcatcg agagatcctc cacaagcgtc catggctcag gtaggcccgg
      541 gccatgaggg gaggctgcaa catccgttcc ggaggcgccg ggacggctca ggccgtccgg
      601 cgctcttcgg gctggacatc gtcgatctcg accgagacga gcttggagac gccgcgctct
      661 tccatggtga cgccgtagag cgtcgaggtg gcttccatag tcagcttgtt atgggtgatg
      721 acgatgaatt gcgtatcgat cgccatctcc ttcaacacgt cgacgaagcg gacggtgttg
      781 acgtcgtcga gcggcgcgtc cacctcgtcc agaatgcaga agggcgacgg cttggtgcgg
      841 aacagggcga agagcagggc gatggcggtc agcgccttct cgcctcccga catcagcatc
      901 agattctgca agcgcttgcc cggagggcgg gccaggatct cgatgccgct ctcgagcggg
      961 tcgttctcat cgagcagccg catctcggcc tcaccgccgc ggaacaggcg ggtgaaggtc
     1021 tcgccgaagc tcttgttgac ctcgacgaag gtggtgttga accgctcgct cgaagcctgg
     1081 ttgatctccc ggatggtgtt cttgaggctc tccaccgagt tggccacgtc ggcgcgctgg
     1141 gtggtgagga actggaagcg ctcctcctgc tcgtcgtgct cctggacggc gaggatgttg
     1201 accggcccca ggcgttccag cgtcgccttg cagcgggcga gctcggcctc catttcggcc
     1261 agcccttccg gggcttcgcc gggctgctcg ggcagcggcc ggctgaactg ctcctggaag
     1321 gtgagggcga ggtgctcagc gtcctgcttg acgctggcct gctggatgcg cagctcctcg
     1381 gcttgggagc ggacctggtc gcgccggtcg cggtggagct ggatgcgctc ctccagatcc
     1441 ttgatctcct gccggtggag gtcgaggcgc tcctgctcct ccagcacccc ctcctcggcg
     1501 gcggcgcgct gctcgagcgc ccgctgcagg tcggcctggg ccttctcgag ctcgccctcg
     1561 atctgccctc ggcggccggt gagcttctcg gcctcttcgc gccaggtggc cacctggcgc
     1621 tggccacgct cgacctcggc ggcgaggcgg gcctcctcgc ggtcgtgcga ctcgaggcgc
     1681 tcgtccagga gctccaggcg gccgcggcgg ccggcgctct cggtgcgaag gctctcgcgg
     1741 tgggccttgg cggcctcgag ctcggcctgg gcgcgctcga acgcctcctg gagggccacg
     1801 ttgcgctcct cggccgcggc gagctcctcc tggagctggc cgcggcgcgc ggcgacgcgc
     1861 tcgatctcgc ggaggatctc ctgctgctcg gagctgagcg tctcctgctc agcggcgagg
     1921 cgccggtggc gggtggcggc gtcctccatc cgggccttgg cgaccgccag ctcctggcgg
     1981 agctgggcca cctctccctg gaggcggttg gactccttgg cgaggttggc ccggtcgttc
     2041 accagccgcg acagctcggc gatcgcctct ttgagctcgg cctcgatctc ggggatggag
     2101 cgggccaggt tcgccagctc gctctcgcgc tccagcacgc ccggggtggc ggtgtcgccc
     2161 tccacgtggt aggtgccggc ctccacccag acgccgtcgc gggagaggaa cgagatgccg
     2221 gggtggcggc gcgccagccg ttcggcgtcg ccgggcgtcc gtaccagaaa ggcggggggg
     2281 agggaggcgg acagctcctc ggggagcccg agggcctcgg cgagggagag gaccaccgcc
     2341 gggtcgtcca ccatcggcgc gacctccggg tccggggtcc gccgctcgat gaggatggcg
     2401 gccgagcggc cggccagcgc cttggcgagc cccagggcgg tctcgcccga ctccatgacc
     2461 acggcgtcgg ccagggcgcc g
//
LOCUS       DASPJI010000657         4916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_609941, whole genome shotgun sequence.
ACCESSION   DASPJI010000657 DASPJI010000000
VERSION     DASPJI010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609941"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /gene="rsmH"
                     /locus_tag="VEL74_18585"
     CDS             complement(<1..825)
                     /gene="rsmH"
                     /locus_tag="VEL74_18585"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HYG64594.1"
                     /translation="MDVQHLPVLVAETVERLAPWRGGLFVDATLGLGGHAEALLTAAP
                     AARLLGIDRDPQALERARRRLAPFGDRVAYAQANFHQLGTVLDRLGIRSISGALADLG
                     VSSLQLETPERGFSFRLDGPLDMRMGLSERTAADVVNQSSEEQLEKILREYGEEKQAR
                     RIARAIVRARDEEPLRTTGQLQKVVERAKGGRGREERIDPATRVFQALRIEVNEELAG
                     LESYIEQTVQLLESDGRLVVISYHSLEDRIVKNTLRDLARGDIDQVTGRPRAESQLI"
     gene            complement(860..1300)
                     /locus_tag="VEL74_18590"
     CDS             complement(860..1300)
                     /locus_tag="VEL74_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HYG64595.1"
                     /translation="MFRGSAPTKIDDKGRLKVPTDFRRLIEERYGADLFVTSVQGDSA
                     LLYPLPVWEEFEARLLALPGTDRTRARFLERVNYFGQQVQMDNQGRLVIPQILRERAG
                     MNGDVVVSGQLDHLVVWNHERFLNRLDQEPFTDDDFRALSERGI"
     gene            complement(1566..2807)
                     /locus_tag="VEL74_18595"
     CDS             complement(1566..2807)
                     /locus_tag="VEL74_18595"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HYG64596.1"
                     /translation="MTNRRRALSLALLLLFTANGLRAETLNGVILRINDQIATLYDYQ
                     QRRQEAVREISARVQDPAERRELLAQAPEIVFRDLYEQLLLDSRADQLGIFISDKEVD
                     ETIAGMRADYGIETDEQFARALQQAGMSLPLLREQMRRNLRLQAVRGREIRPRVQVDE
                     EDLRRYYRKNEEQFRLPEQVQVREVVVRDGSEPNAQKVLAPEERLRIAEAIRSEVTAG
                     KPLADAVAPYVQTGVATNVVEFGWVARGDLDPTLEAVAWDLQAGQVSAPVEGRGGLHV
                     LQVTERRPSRIAEFSEVAEQIREREQNRVYREEVAKYMQELEQKALIVANPPQEAAGF
                     RRLLGGSPETDTMGNEVDPMSTAAPASGATAEEAVESAVSEAPRPQDRDTGQNAPQAP
                     GTLPEPKPITDAPPATPPPGA"
     gene            complement(2782..3687)
                     /locus_tag="VEL74_18600"
     CDS             complement(2782..3687)
                     /locus_tag="VEL74_18600"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYG64597.1"
                     /translation="MRHPAAAALLVLVPLGLVGAASCGRPRPPGPDVVARIGDGEVRY
                     AQFEAYLRHAVGTMDSAGDGGEAEGEGVLASDALSGLFDQFLDEELLARLAAERGLMA
                     GAEADAAGRRRAVDRLLESLAAGEPSEEEIAAYYREHRGEFARPERVRLRQILTDERA
                     AAEGALRELAAGADFAELARRVSRDPSAASGGEQGELARADLPPAFAEVIFALSPGEV
                     SRLVPADYGFHVFQVTERLPAEVVPFPQVRGEIAERLRRERADRRLRELVEEARSAHD
                     VTVYERNLPFNYKGFYGHDKPTPSP"
     gene            complement(3684..>4916)
                     /locus_tag="VEL74_18605"
     CDS             complement(3684..>4916)
                     /locus_tag="VEL74_18605"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HYG64598.1"
                     /translation="LVIVDEEQRFGVAQKERLKQLKKNIHVLAMSATPVPRTLQMSLA
                     GVRDLSVIETPPKDRMAVETAILPFNPQLVREAIEVEMERGGQVYYVYNRVESIEEML
                     MELKKIVPKARITVGHGQMDEEELSRRMHAFTRGDYDVLLATTIIENGIDIPNVNTMI
                     VHRAERFGLSQLYQLRGRVGRSNQLAYCYLLVPEDRVLSEQARKRLAAIREFTDLGAG
                     FRIAARDLEIRGAGNMLGGEQSGHIGSLGIDTYLKLLEETVRELRGEVVEEAPSVAID
                     LPVPMSIPTDYVDDANLRMEIYRKIAAADVPESDILAELTDRFGPPPPAVSTLLQVAT
                     LKRLAESLRVQSISSKGSELIIRLRRDARIDVERLIEMVSNRPGASFSPTGVLTVAVG
                     SGGGMLGVARETLEVLSQ"
BASE COUNT          691 a         1788 c         1674 g          763 t
ORIGIN      
        1 gatgagctgg ctctccgccc gcggccggcc ggtgacctgg tcgatgtccc cccgcgccag
       61 gtcgcgcagg gtgttcttga cgatgcggtc ctccaggctg tggtaggaga tgacgaccag
      121 ccggccgtcc gactccagga gctgcaccgt ctgctcgatg tagctctcga gcccggcgag
      181 ctcctcgttg acctcgatgc gcagcgcctg gaacacgcgc gtcgccgggt cgatccgctc
      241 ctcccggccg cgccccccct tggcccgctc caccaccttc tggagctggc ccgtcgtgcg
      301 cagcggctcc tcgtcccgcg cccggacgat ggcgcgcgcg atcctccgtg cctgcttctc
      361 ttcgccgtac tccctcagga tcttttccaa ttgctcctcc gagctctggt tgaccacatc
      421 ggccgcggtc cgctccgaaa ggcccatccg catgtccaaa ggcccgtcga gacggaaact
      481 gaagccccgc tccggcgttt cgagttgcag tgaagagacg cccaggtcgg ccagggcgcc
      541 ggagatgctc cggatcccga gccggtccag gaccgtcccc agttgatgga aattggcctg
      601 cgcgtaagcc acccggtcgc cgaacggagc gaggcgccgc cgcgccctct ccagcgcctg
      661 cggatcccgg tcgatcccca ggagccgcgc cgcgggagcc gccgtgagca gcgcctcggc
      721 gtgccctccc agccccaggg tcgcgtcgac gaaaaggccc cccctccagg gagcgagccg
      781 ctccacggtc tcggccacga gaacaggcag gtgctggaca tccaacggct cgggcggcct
      841 ttccgggcct ggggcccgct cagatcccgc gctcgctcaa agcacggaag tcgtcgtccg
      901 tgaacggctc ctgatccaga cggttcagga agcgctcgtg gttccacacc accaggtggt
      961 cgagctgtcc gctcaccacc acgtcaccgt tcatgccggc ccgctcccgc aggatctgcg
     1021 ggatgacgag ccggccctgg ttgtccatct gcacctgctg tccgaagtag ttgaccctct
     1081 ccaggaaccg cgcccgcgtc cggtcggtgc ccggcagcgc caggaggcgc gcctcgaact
     1141 cctcccagac cggcagcggg tagagcagcg ccgaatcgcc ctgcaccgac gtcacgaaca
     1201 ggtccgcccc gtagcgctcc tcgatcaggc gacggaagtc cgtggggact ttcaatctcc
     1261 ctttgtcgtc gatcttggta ggtgcgcttc cgcggaacat aggaccgcca tcgtctaggg
     1321 tcgaatccta gcagttcagt ccacttttgt ccattttttt ccaccagaac ccacggcgag
     1381 accaccccag cccactcctg gtccaccata ggggcgaaaa tacaccgttg ctggggcctg
     1441 gaaggccggt atgggaaagg aaaaccaggt ctggaggggg agatttgtag gaaagaaaaa
     1501 gggggacggg aggggctccc tcccccgggc gggggaggaa gcggctcaag gcctcagacg
     1561 ctgcctcagg cgccgggcgg cggcgtggcg ggcggggcgt cggtgatcgg cttgggctcc
     1621 gggagggtgc ccggcgcctg cggcgcgttc tgcccggtat cccggtcctg cggccgcggg
     1681 gcctcgctca cggcgctctc caccgcctcc tcggcggtgg cgcccgaggc cggagccgcg
     1741 gtggacatcg gatcgacctc gttgcccatg gtgtccgtct ccggcgagcc gcccagcagg
     1801 cggcggaagc cggcggcctc ctggggagga ttggcgacga tgagcgcctt ctgttccagc
     1861 tcctgcatgt acttggcgac ctcttcgcga tagacccggt tctgctcgcg ctcacggatc
     1921 tgctcggcca cctccgagaa ctcggcgatg cgcgagggcc ggcgctcggt cacctgcagc
     1981 acgtgcaggc cgccgcggcc ctccaccggc gcgctgacct gtccggcctg gaggtcccag
     2041 gcgaccgcct ccagggtagg atcgagatcg ccccgggcga cccagccgaa ctcgacgaca
     2101 ttggtcgcca ccccggtctg cacgtagggg gcgacggcgt cggccagcgg cttgccggcg
     2161 gtcacctcgc tacggatcgc ctcggcgatg cgcagccgct cctccggcgc caggaccttc
     2221 tgcgcgttgg gctcgctgcc gtcgcggacc accacctcgc gcacctggac ctgctccggc
     2281 agacggaact gctcctcgtt cttgcggtaa tagcgccgca ggtcctcctc gtccacctgg
     2341 acgcgggggc ggatctcgcg cccacgcacc gcctggagcc gaaggttgcg ccgcatctgc
     2401 tcgcgcagaa gggggagcga catcccggcc tgctgcagcg cccgcgcgaa ctgctcgtcg
     2461 gtctcgatgc cgtagtcggc ccgcatcccg gcgatggtct cgtcgacctc cttgtcgctg
     2521 atgaagatcc cgagctggtc ggcccgcgag tccagcagga gctgctcgta caagtcccgg
     2581 aagacgatct ccggcgcctg ggcgagcagc tcgcggcgct cggccgggtc ctgcacgcgg
     2641 gcggagatct cccgcaccgc ctcctggcgg cgctgctggt agtcgtacag ggtggcgatc
     2701 tgatcgttga tgcgcaggat caccccgttc agcgtctcgg ctcgcaggcc attggcggtg
     2761 aagaggagga gcagcgcgag gctaagggct cggcgtcggt ttgtcatgac catagaaacc
     2821 tttgtaattg aagggaagat tccgctcgta gacggtgacg tcgtgggcgc tccgggcctc
     2881 ctccaccagc tcccgcagcc gccggtcggc gcgctcgcgg cgcagccgct cggcgatctc
     2941 gccgcgcacc tgagggaagg ggaccacctc ggcggggagc cgctcggtca cctggaagac
     3001 atggaagccg tagtcggcgg gcaccagccg gctgacctcg ccggggctca gggcgaagat
     3061 gacctcggcg aaggccggcg gcaggtcggc gcgcgccagc tccccctgct cgccgcccga
     3121 cgcggcgctg gggtcgcgcg acacccggcg cgccagctcg gcgaagtcgg cgccggccgc
     3181 cagctcgcgc agggcgccct cggccgcggc gcgctcgtcg gtcaggatct gccgcagccg
     3241 cacccgctcg gggcgggcga actcgccgcg gtgctcgcgg tagtaggcgg cgatctcctc
     3301 ctcggagggc tcgccggccg ccaggctctc cagcagccgg tccaccgccc ggcgccggcc
     3361 ggcggcgtcg gcctccgcgc cggccatcag cccgcgctcg gcggcgagcc gcgccagcag
     3421 ctcctcgtcg aggaattgat cgaagaggcc ggagagggca tcgctcgcca gcaccccttc
     3481 gccctccgcc tctccaccgt cgccggcgct gtccatcgtg ccgacggcgt ggcggagata
     3541 ggcctcgaac tgcgcgtagc ggacctcgcc gtcgccgatc cgggccacca cgtcggggcc
     3601 gggcggccgg gggcggccgc aggacgccgc tcccacgagg ccgaggggca cgaggaccag
     3661 cagggccgcc gccgccggat gcctcactgc gacagcacct ccagggtctc gcgcgccacc
     3721 ccgagcatgc cgccgccgct ccccaccgcc acggtgagca cgccggtggg gctgaagctc
     3781 gcgcccggcc ggttcgacac catctcgatc agccgctcga cgtcgatgcg cgcgtcgcga
     3841 cgcagccgga tgatcagctc cgagcccttg ctggagatcg actggacacg cagcgactcg
     3901 gccaggcgct tcagcgtcgc cacctgcagc agcgtcgaca ccgccggcgg cggcggcccg
     3961 aagcggtcgg tcagctcggc caggatgtcg gactccggca cgtcggcggc ggcgatcttg
     4021 cggtagatct ccatgcgcag gttggcgtcg tccacgtagt cggtggggat cgacatcggc
     4081 accggcaggt cgatggccac cgacggcgcc tcctccacca cctcgccgcg cagctcgcgc
     4141 accgtctcct cgagcagctt cagataggtg tcgatgccga gcgagccgat gtggccgctc
     4201 tgctcgcccc ccagcatgtt gccggcgccg cggatctcga ggtcgcgggc ggcgatgcgg
     4261 aagccggccc cgaggtcggt gaactcgcgg atcgccgcca ggcgcttcct cgcctgctcg
     4321 gagagcaccc ggtcctcggg caccagcagg tagcaatagg cgagctggtt gctccgcccc
     4381 acccggccgc ggagctggta gagctgcgac aggccgaagc gctcggcgcg gtggacgatc
     4441 atggtgttga cgttggggat gtcgatgccg ttctcgatga tcgtcgtcgc cagcagcacg
     4501 tcgtagtcgc cgcgggtgaa cgcgtgcatg cgccgggaga gctcctcctc gtccatctgc
     4561 ccgtggccga cggtgatgcg ggccttgggg acgatcttct tgagctccat gagcatctct
     4621 tcgatcgact cgacgcggtt gtagacatag tagacctgtc cgccccgctc catctcgacc
     4681 tcgatcgcct cgcgcacgag ctgggggttg aatggcagga tggccgtctc caccgccatg
     4741 cggtccttgg gcggcgtctc gatcaccgag agatcccgca cgccggcgag cgacatctgg
     4801 agcgtgcgcg gcaccggcgt cgccgacatg gcgagcacgt ggatgttctt cttgagctgc
     4861 ttgagccgct ccttctgggc gacgccgaag cgctgctcct cgtcgacgat gaccag
//
LOCUS       DASPJI010000658         3199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_42605, whole genome shotgun sequence.
ACCESSION   DASPJI010000658 DASPJI010000000
VERSION     DASPJI010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42605"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3199
                     /locus_tag="VEL74_18610"
     CDS             <1..>3199
                     /locus_tag="VEL74_18610"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64599.1"
                     /translation="VELPLRAVFEAPTLSGLAPRVEALLAGGRRAEVPPVLPVPRDDG
                     TLPLSFAQERLWFLDRLEPGSSVYNMPGVLALHGALDVAALELALGELVRRHESLRTT
                     FTEAGGEPRQVIAPVLGASLPVVDLSALSAGSREVEAHRLAAAEAARPFDLERGPLLR
                     ASLLRLAHEEWLLLVTLHHIVSDGWSMAVLVRETGLLYGAFSRRLPPPLPELAIQYPD
                     FAVWQRQWLSGEVLEAQLFYWRSALVGAPSVLELPTDRPRPAVRSFRGAGCALELPGD
                     VSSGVTALGRRHGATLFMTSLAAFQALLWHYTGQQDLPVGTVVANRTRPEIEGLIGFF
                     VNTLVLRGRLDRLGGGASRFAEVLAAARVTCLEAYAHQDLPFERLVEELKIERSLSHS
                     PLFQVLLVLQNVPDETLELADLTLAPVDSGGGTAKFDLSLTLGESGGRLGASLEYSTD
                     LFDAATARRLLEHFQVLLAAAAADPDRLVRDLPLLTAGERHQLLAEWNDTLPLTGTEI
                     SLPEAIANVAERLPDVVSLAAEGTSLSYREMEVWTGRLARWLRRRGVGPESRVGVCLE
                     RSPELIMALLAVQKAGGAYVPLDPDYPRERLAYVLEDAAVVLVITESRLLGTLPELEE
                     DRLCRLDREWDALPVLDDGDIPAPVLLPQHPAYLIYTSGSTGRPKGTVVTHGSALNHM
                     RWMVPAMSMGPGQRLLLKTPVSFDASVLEVFVPPMAGATMVTARPDGHRDPSYMVEAL
                     QRHEITVVQAVPKLLRAVSMEPRLEDCVALRILLVGGEALERELAETLQARIGCEVWN
                     LYGPTETTVDTTYERLSPGPGTSVPLGRQITGAWLYVLDGELLPVPIGVAAELYIGGA
                     GPARGYLARPELTAERFLPNPWSPIPGDRMYRAGDLVRRLPDGRLDYLGRTDHQVKVR
                     GFRIEPGEIEAALRDSPGVRESVVLVREDIPGDPRLVAYVVTGEGDERPGSEDLRAAL
                     RQRLPEYMVPTSIVLLEALPVLPNGKVDRRSLPAPELERATTGGVAPRTPTEELLAGI
                     WADLLGLERVGAGDDFFALGGHSLLGTRMVSRLRDA"
BASE COUNT          378 a         1174 c         1150 g          497 t
ORIGIN      
        1 gtggagctgc cgttgcgtgc cgtcttcgag gcgcccaccc tctccggcct cgctccccgt
       61 gtcgaggccc tgctggccgg cgggcgacgg gccgaggtgc cgccggtcct gccggtccct
      121 cgggatgacg ggacgctgcc cctctccttc gcccaggagc gcctctggtt cctcgaccgc
      181 ctggagcccg gcagcagcgt ctacaacatg ccgggcgtgc tcgcgctcca cggcgcgctc
      241 gacgtcgccg ccctggagct cgcgctgggc gagctcgtcc ggcgtcacga gtccttgcgc
      301 acgacgttca ccgaggccgg cggcgagccg cgccaggtca tcgctcccgt gctgggagcc
      361 tcactccccg tggtggacct gagcgctctc tccgctggct cgcgggaagt ggaggcgcac
      421 cgactcgccg ccgcggaggc ggcacggccg ttcgacctcg agcggggccc gctgctccgc
      481 gccagcctgc tgcggctcgc gcacgaggag tggctcctgc tcgttaccct gcaccacatc
      541 gtctccgacg gctggtccat ggcagtgctc gtccgcgaga ccggcctgct ctacggcgcc
      601 ttctcgcggc ggctcccgcc gcctctgccg gagctggcga tccagtaccc ggatttcgcg
      661 gtgtggcagc gtcagtggct ctccggcgag gtgctggagg cccaactctt ctactggcgg
      721 tcggcgctgg tgggcgcccc gtcggtcctc gagctgccga ccgaccggcc gcgcccggcg
      781 gtacgtagtt tccgcggcgc cggctgcgcc ctggagctgc cgggcgacgt ctcctccggc
      841 gtcaccgcgc tcggccggcg gcacggcgcc accctgttca tgacctctct cgccgcgttc
      901 caggcgcttc tctggcacta cacgggccag caggacctgc cggtcggcac ggtggtcgcc
      961 aaccgtaccc ggccggagat cgaggggctc atcggcttct tcgtcaacac cctggtgctg
     1021 cgggggcggc tcgaccggct gggcggcggc gcttcgcgct tcgcggaggt gctggcggcg
     1081 gcgcgcgtca cctgcctgga ggcctacgcc caccaggacc tacccttcga gcggcttgtc
     1141 gaggagctca agatcgagcg gagcctctcc cacagcccgc tgttccaggt cctcctcgtg
     1201 ctgcagaacg tccccgacga gaccctggag ctggcggacc tgaccctggc gccggtggac
     1261 agcggcggtg gcaccgccaa gttcgacctc agcctgaccc tcggcgaatc cggcggccgg
     1321 ttgggggcct ccctggagta cagcacggac ctgttcgatg ccgccaccgc gcggcggctg
     1381 ctcgagcatt ttcaggtgct cctcgccgcg gcggcggccg accccgaccg cttggtccgc
     1441 gacctcccgc tgctcaccgc gggcgagcgc catcagctcc tggcggagtg gaacgacacg
     1501 ctgccgctga ccggtacgga gatcagcctg cccgaggcga tcgccaacgt ggcggagcgg
     1561 ctcccggacg tcgtctctct cgccgccgag gggacctccc tgagctatcg cgagatggag
     1621 gtctggaccg gccgcctggc acggtggctc cggcgccggg gtgtcggccc cgagtcccgg
     1681 gtgggcgtct gcctggagcg gtcgccggag ctgatcatgg ccctgctggc ggtgcagaag
     1741 gccggcggcg cgtacgtgcc gctcgatccc gactacccgc gcgagcgcct ggcctacgtg
     1801 ctggaggacg ccgcggtggt gctggtgatc accgaatcgc gcctgctggg gaccctgccc
     1861 gagctagagg aggaccggct ctgccgtctc gaccgggagt gggatgctct gccggttctc
     1921 gatgacggcg acatcccggc gcccgtcctc ctgccgcagc atccggctta tctgatctat
     1981 acctcgggtt cgacgggacg gcctaagggg acggtggtca cccacggctc ggccctcaac
     2041 cacatgcgct ggatggtgcc ggcaatgagc atggggccgg ggcagcggct gctgctcaag
     2101 accccggtga gcttcgacgc ctcggtgctg gaggtcttcg tgccgcccat ggcgggtgcc
     2161 accatggtca ccgcccggcc ggacgggcac cgcgacccgt cctacatggt ggaggcgctg
     2221 caacggcacg agatcacggt cgtccaggcg gtccccaagc tgctgcgggc tgtgtccatg
     2281 gagcccaggc tggaggattg cgtggctctg cgcatcctcc tcgtcggcgg cgaggccctg
     2341 gagcgggagc ttgccgagac gctgcaggcc cgcatcggct gcgaagtgtg gaacctctac
     2401 ggtcccaccg agaccaccgt cgacaccacc tatgagcggc tgtcgccggg gccggggacc
     2461 agcgtgccgc tgggccggca gatcaccggc gcctggctct acgtgctcga cggcgagctc
     2521 ctgcccgtac ccatcggcgt ggcggcggag ctctatatcg gcggcgccgg cccggcgcgc
     2581 ggctacctgg cacggccgga gctgacggcg gagcgcttcc tgcccaaccc gtggagcccc
     2641 atccccggcg accggatgta ccgcgccggc gacctggtcc gccgtctgcc cgatggccgg
     2701 ctcgactacc tgggccgcac cgatcatcag gtcaaggtgc ggggcttccg catcgagccc
     2761 ggcgagatcg aggccgcgct gcgggatagc ccgggagtcc gggagtccgt ggtgctggtc
     2821 cgcgaggaca tccccggcga tccgcggctg gtcgcctatg tggtgacggg ggagggggac
     2881 gagcggccag gctcggaaga tctgcgcgcc gccctgcgcc agcggctgcc cgagtacatg
     2941 gtccccacct ccatcgtgct gctggaggcg ctgccggtgc tgccgaacgg caaggtggac
     3001 cgccggtcgc tgcccgcgcc ggagctggag cgggcaacga ccggcggggt ggcgccgcgc
     3061 acccccaccg aggagctgct ggccggcatc tgggccgacc tcctggggct ggagcgggta
     3121 ggggccggcg acgacttctt cgccctcggc ggtcattcgc tcctgggcac ccgcatggtc
     3181 tcgcgcctgc gggacgcct
//
LOCUS       DASPJI010000659         8959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_389291, whole genome shotgun sequence.
ACCESSION   DASPJI010000659 DASPJI010000000
VERSION     DASPJI010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8959)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8959
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389291"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..270)
                     /locus_tag="VEL74_18615"
     CDS             complement(16..270)
                     /locus_tag="VEL74_18615"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HYG64600.1"
                     /translation="MGRLLGLVLLAFLIWLCLRALRRFLQAPEAPKDARSLPVETLVR
                     CAACGVYVSGERTLPGERGGAYCSEACRRQAEAASASSSS"
     gene            complement(279..893)
                     /locus_tag="VEL74_18620"
     CDS             complement(279..893)
                     /locus_tag="VEL74_18620"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG64601.1"
                     /translation="MTKDNLIFAVFGILLGFIAGYLLHEVMAARQPARLTAGAVVQGM
                     PQQGVPPGNAGGAGAPSGPGPAMEQVQQLSRRVQENPNDADAVLQLANMNFEIGQWAR
                     ARDLYSQYLTLRPENPDVLSDIGVTYREQGQFDQALDMFRRAHALDSSHWRSLFNQVV
                     ILGIDLERYDEAEEALQKLRQLQPGNSDVERLATEVEQRRSQRS"
     gene            complement(898..1713)
                     /locus_tag="VEL74_18625"
     CDS             complement(898..1713)
                     /locus_tag="VEL74_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64602.1"
                     /translation="MVRRLAAPLALLSLLLVTYGVIAQDDQADTAAPVAATASQRAQV
                     EMQLRLEQRLLVLDLAGYDEARTRERQALERMGEVATRLDETLGGDSLALGTLEALRD
                     ELDGAREAGRTAAGRVDAQIRRLEERLQRIGFLEGELSGRTAAAPRRTDVITGRWRVR
                     VQPQDRPGTFELRLSGTVVTGTYSIEGGGSGSLQGTYAANRLRLERIDVRRGFDSVFE
                     GTLTGSGVLTGTWTANELGLPEPAQGGWSAVRISSEDAGETADEDDAGDDGEP"
     gene            complement(1811..2329)
                     /locus_tag="VEL74_18630"
     CDS             complement(1811..2329)
                     /locus_tag="VEL74_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HYG64603.1"
                     /translation="MTFKVGQKVVYPNHGVSLIEKIEPTQIDGVEQTYYHLRLLANNS
                     KVMVPKSNLDLVGLRPLCQNREVRALFEILEDGNIDTYKDWKGRYKQNLDKMKTGRLT
                     DVAEVLKNLRLVSQRKSLSFREKKMYERAKYFIVSEVAHVKAITERESEDLVERALNS
                     SLEKRQRTKVAS"
     gene            2546..2632
                     /locus_tag="VEL74_18635"
     tRNA            2546..2632
                     /locus_tag="VEL74_18635"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2579..2581,aa:Leu,seq:caa)
     gene            complement(2638..2820)
                     /locus_tag="VEL74_18640"
     CDS             complement(2638..2820)
                     /locus_tag="VEL74_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64604.1"
                     /translation="MESDLNAARERDTPLRDRARAAVTRLLGRLRPEGPCLRCAATGA
                     WQGGLDCPDCGGTGRR"
     gene            complement(2835..4058)
                     /locus_tag="VEL74_18645"
     CDS             complement(2835..4058)
                     /locus_tag="VEL74_18645"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG64605.1"
                     /translation="MVFLENFRVALEALRANKMRSILTTLGIIIGVAAVIAVVSIVQG
                     LQYTITNELQGVGATFMVIQRDMQRNRPDMVVRQVRLTWEDGQALRERVPGIRLITPA
                     MFGQSQVKYRERKHTPFAIIGVNQDWQDVNNHTVEQGRFFSRVDLENRRKVTVVGRKV
                     VEELALGDDPIGKEIYIGTYPATVIGVMEKKGRAFGFDQDDMVFVPFDSAVALFGRNA
                     ADAVELRLQARDTQSVEQVKDGITQLLRTRHQIPEGQPNDFQIQTQDEILDSVNSILG
                     SVTAVVGGIVGIALLVGGIGIMNIMLVSVTERTREIGVRKAVGARRQDVLVQFLIEAV
                     TLSLVGGGLGLAIGYGLGVLAASVLPGDFPPAHVPLWAVFLAFGFSAMVGIFFGIYPA
                     AKASRLDPIEALRYE"
     gene            complement(4086..4796)
                     /locus_tag="VEL74_18650"
     CDS             complement(4086..4796)
                     /locus_tag="VEL74_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG64606.1"
                     /translation="MALIELRDIHKVYDMGAEQVRALNGVDLSVERGEYVAIMGSSGS
                     GKSTLMNLLGCLDTPSSGSYVLNGTAVQELDDQQLAGIRNKEIGFVFQTFNLLARTDA
                     LHNVELPLVYAGLSRSERRERARRALERVGLAQRMHHQPNELSGGQRQRVAIARALVN
                     DPSILLADEPTGNLDSATSEEIMRLFDELHQAGNTVVLVTHEADIAEHAWRRVTLRDG
                     KVLSDQPTGRASRSHAHA"
     gene            complement(4796..6085)
                     /locus_tag="VEL74_18655"
     CDS             complement(4796..6085)
                     /locus_tag="VEL74_18655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYG64607.1"
                     /translation="MKKILVIVGVLLLLGAIVFASLKNAGREKGERVYAEEAARRDLT
                     QIVKASGEIDPRIKVNISAHVIGKIEKLYVEEGDLIQAGQPFLQLEREAFLAQRNQWA
                     AQLRSAQTGVRQAEVSLADARHKLERARRLTQEGITSNEQLEAAELAETSARLRVDEA
                     RESVLQMQANLEKAQDDLSKTTIFAPLTGRVIALNAEQGEVVVSGTMNNAGSIIGTIA
                     DLSEILANVDVDETEIVNVRMGQEAVLRVDALPNRVYHGRVVEIGSSGFNRPQQPDVT
                     FYEVKVLLTDSDEALRPGMSVRAEIQTAAHANALVLPIQAVVERPPLTAEGEEAKAEE
                     DDEIKVVFVLEDGKARQRTVETGLSDETHVEIRSGVKPGERVVTGPYRTLRDLRDGDA
                     VTVSTTSEAADRRADRERDRAENRGEDAATDDQGEQD"
     gene            complement(6082..6810)
                     /locus_tag="VEL74_18660"
     CDS             complement(6082..6810)
                     /locus_tag="VEL74_18660"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HYG64608.1"
                     /translation="MHDSSFGRLLGVLVAPGKTFRSIAERPTWLAPLLVLLVLGTTAA
                     YMMQQRVDQEELVRHQVSQFGMGNVSDDQIQEMVERNEKMGPVTLLVGPIMTAVVFLI
                     VALVFWVAFKMVGSEMDFKSSLATSVHGLMPSAISLLLSILILLGRSTVTPEEAMSGG
                     ILTSSAATVAPEEASPVVKSLLGSLDVFSIWSLILLVIGYRVVAKVSPKAAAITVIAL
                     WLLYVLGKAGFAAAIGSMAGGAGS"
     gene            complement(6826..8550)
                     /locus_tag="VEL74_18665"
     CDS             complement(6826..8550)
                     /locus_tag="VEL74_18665"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYG64609.1"
                     /translation="MTRTSLWFHTGDVRPARRLTAVLVTASMVGGLLVAPGASAQTTT
                     PQTTTQTQTTTQSQTPTQTPPAVPAPTVLQTTPPPEPLVGQPLVEVGNGAIQLSLEQA
                     IAVALRQNLDLASQLYVREQQRLAIQQALGIYDLRTDATLSASEDESDPENPTLPVSS
                     EEQSLDFGFSQLAPWGGDFSIGVDQRRSSFSFRPGTFRDVPVTYGGGLTFGYTQPLLR
                     NFGRLATERGLMLAQLDNRISREEFERQVTLTIQQVVNAYWDLVGAREQLAVAEESMA
                     LAREFHERNRIQVEVGTLAPLELVQSEAAIAERDEDIISAQATLGDAADELRRLLNLP
                     PGELWEVPINAVTSPESAERLTFNVEEAVSTAFAERPELQQQQFQLEQNQINAAFFRN
                     QTRPRLDLSVSYGSSAGSQEALSDVYSDLFGLDLTGWATSLVFAYPLQNRSARAQSAS
                     ANLGVERSETELENLQDRIRTEVRQAARRVDTAAKQIEAARASVRFQERNLEAERKRY
                     ENGMSTSFEITQIQEDLTLARSREVNATIAYQTALTEYYRATGTLLEQQGVELVDPEQ
                     PINRFTLF"
     gene            complement(8670..>8959)
                     /locus_tag="VEL74_18670"
     CDS             complement(8670..>8959)
                     /locus_tag="VEL74_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG64610.1"
                     /translation="EPISALDVSVRAQILNLLAGLQERLGLTLLLIAHDLAAVEQVAD
                     RVAVLHRGRLVEVGPREEVFARPRHPYTAGLLAAVPVPDPGRRRHGGAGPT"
BASE COUNT         1332 a         3099 c         3055 g         1473 t
ORIGIN      
        1 gccaagagag tggcgctatg aagaggaaga ggcggaggcc gcttcggcct ggcgccggca
       61 ggcttccgag cagtaggccc ctcccctctc ccccggcagg gtccgctccc cggagacgta
      121 cacgccgcag gcggcgcagc gcaccagcgt ctcgacgggt agcgagcggg cgtccttcgg
      181 agcttcggga gcctgcagga agcggcgcaa ggcccgcagg cagagccaga tcagaaaagc
      241 cagcaggacg aggccgagca ggcgccccat accccgcctc aggagcgctg gctccggcgc
      301 tgctcgacct cggtcgccag cctctcgacg tcgctgttgc ccggctgcag ctgccgcagc
      361 ttctggagag cctcctcggc ctcgtcgtac cgctcgaggt cgatgcccag aatcaccacc
      421 tggttgaaca gcgaccgcca atgggaagag tccagggcgt gtgcgcggcg gaacatgtcc
      481 agggcctggt cgaactgccc ctgctcccgg taggtgacac cgatgtcgga gaggacatcg
      541 ggattctccg gccgtaaggt gaggtactgg ctgtagaggt cgcgcgcccg ggcccactgc
      601 ccgatctcga agttcatgtt ggccagttgc agcacggcgt cggcatcgtt ggggttctcc
      661 tgcacgcgcc ggctgagctg ctggacctgc tccatggccg ggccggggcc ggagggcgcc
      721 cccgcgccgc cggcgttccc cggcggcaca ccctgctgcg gcataccctg gaccacggct
      781 ccggcggtca gccgggcagg ctggcgcgcg gccatgacct cgtgcagcag gtacccggcg
      841 atgaagccga gaaggatgcc gaacaccgcg aaaatcagat tgtctttggt caagatctca
      901 aggctctccg tcgtcgccgg catcgtcctc gtccgcagtc tccccggcgt cctcactgct
      961 gatgcggacg gcgctccagc cgccctgggc cggctcgggg aggccgagct cgttggcggt
     1021 ccacgtaccg gtgaggacac ccgagccggt cagcgtgccc tcgaacacgc tgtcgaagcc
     1081 acggcgcacg tcgatgcgct cgaggcgcag gcggttggcg gcataggtcc cctgcaggga
     1141 gccggagccg ccaccctcga tcgaataggt accagtgacc acggtaccgg agagtcgcag
     1201 ctcgaaggtc cccggcctgt cctgcggctg gacgcgcacc cgccagcgcc cggtgatgac
     1261 gtcggtccgc cgcggcgccg ccgccgtcct cccgctcagc tccccttcca ggaagccgat
     1321 gcgctggagg cgctcctcca ggcggcggat ctgcgcgtcg acccggccgg cggcggtacg
     1381 ccccgcctcg cgggcgccgt ccagctcgtc gcgcagcgct tccagggtgc ccagggcgag
     1441 gctgtccccc cccagcgtct cgtcgagccg agtggcgacc tcccccatcc gctccagcgc
     1501 ctgccgctct cgggtgcgtg cctcgtcgta gccggcgagg tccagcacca gaagccgctg
     1561 ctccagccgg agctgcatct ccacctgcgc ccgctgcgac gccgtggctg ctaccggcgc
     1621 ggccgtatcc gcctgatcgt cctgggcgat gaccccatag gtgacgagca ggagggacag
     1681 cagggcgagc ggggcggcga ggcgtcggac catggcgggg ggattctagc agcgtctccg
     1741 ggcgggaacg acgaaggccg ctccggggag cggccttctc tcgaatccgg tccggcccgg
     1801 agaccggaga tcagctcgcc actttggtgc gctggcgctt ctccaggctc gagttcaggg
     1861 cccgctcgac caagtcctcg gactctcgct cggtgatcgc cttcacatgg gcgacctcgc
     1921 tgacgatgaa gtacttcgcc cgctcgtaca tcttcttctc acggaacgaa aggctcttcc
     1981 gctggctgac gagacggagg ttcttgagca cctccgcgac gtcggtcagc cggccggtct
     2041 tcatcttgtc cagattctgc ttgtagcggc ccttccagtc cttgtaggta tcgatgttgc
     2101 cgtcctcgag gatctcgaag agggcgcgta cctcgcggtt ctggcacagc ggacgcagac
     2161 ccaccagatc caggttggac ttgggcacca tcaccttgga gttattggcg agaagcctga
     2221 ggtgatagta ggtctgctcc accccatcga tctgcgtggg ctcgatcttc tcgatcaggc
     2281 tcacaccgtg gttggggtaa accacctttt gaccgacctt gaatgtcacg tcacgaaccc
     2341 ccttgaattt tcagaaacag aagatggagc tgcggatagt aagtcgctga gggacttccg
     2401 cttacgcact tctttataca ccaggacgga ccgcctggtc aacatcgaag cgtcaggggg
     2461 acggtagaag cgcgtaagcc caggggtggc gcggttgaca caccgaaggt caggccctac
     2521 cattccccgg ctggcccacc ccgttgccgg agtggcggaa tggcagacgc aggggactca
     2581 aaatcccccg ccccttgcgg gcgtgtgggt tcgactccca tctccggcac caacgtctca
     2641 ccgtctcccg gtaccgccgc agtcggggca gtccaggccc ccctgccagg cgccggtggc
     2701 ggcgcagcgc agacaggggc cctcgggccg caggcgtccg agcagccggg tcacggccgc
     2761 cctcgcccgg tccctcagcg gggtgtccct ttcccgtgcg gcgttcaggt cgctctccat
     2821 cctctccgat tgtactactc gtagcggagc gcctcgatgg gatcgaggcg cgacgccttg
     2881 gccgccgggt agatgccgaa gaagatcccc accatggccg agaagccgaa ggccaggaac
     2941 accgcccaga ggggcacgtg ggccggcggg aagtcaccgg gcaggacgct cgccgccagc
     3001 acgcccaggc cgtagccgat ggcgagcccc aggcctcctc ccaccagcga cagggtgacc
     3061 gcctcgatga ggaactggac gagcacgtcc tggcggcggg ctcccaccgc cttgcgcacg
     3121 ccgatctccc gcgtccgctc ggtcaccgac accagcatga tgttcatgat gccgataccg
     3181 cccaccagca gggcgatgcc gacgatcccg ccgaccacgg cggtgacgct gcccaggatg
     3241 ctgttgacgc tgtcgaggat ctcgtcctgg gtctggatct ggaagtcgtt cggctgcccc
     3301 tcggggatct ggtggcgggt ccgcaggagc tgggtgatgc cgtccttgac ctgctcgacc
     3361 gactgggtgt cccgggcctg cagccgcagc tccaccgcgt cggcggcgtt gcggccgaag
     3421 agcgccacgg ccgagtcgaa ggggacgaag accatgtcgt cctggtcgaa gccgaaggcc
     3481 ctccccttct tctccatgac gccgatcacc gttgccgggt aggtgccgat gtagatctcc
     3541 ttgccgatgg ggtcgtcgcc cagggcgagc tcctccacca ccttgcggcc caccaccgtc
     3601 accttgcgcc ggttctccag gtcgacacgg gagaagaagc gcccctgctc caccgtgtgg
     3661 ttgttgacgt cctgccagtc ctggttgacg ccgatgatgg cgaagggtgt gtgcttgcgc
     3721 tcgcggtact tgacctgcga ctggccgaac atcgccgggg tgatcaggcg tatgcccggc
     3781 acccgttccc ggagcgcctg gccgtcctcc caggtgagcc ggacctggcg gacgaccatg
     3841 tcgggccggt tgcgctgcat gtcgcgctgg atcaccatga aggtggcgcc caccccctgg
     3901 agctcattgg tgatggtgta ctgcaacccc tggacgatgg acaccacggc gatgaccgcg
     3961 gcgacgccga tgatgatccc cagcgtcgtg aggatcgagc gcatcttgtt ggcgcgcaag
     4021 gcctccagag cgactcgaaa attttccagg aagaccaacg gcggctcctc tctcgggcgg
     4081 cggcgtcagg cgtgggcgtg cgaccgggac gcgcggcccg tgggctggtc ggagagcacc
     4141 ttgccgtcgc gcaacgtaac ccggcgccag gcgtgctcgg cgatgtcggc ctcgtgggtg
     4201 accagcacca ccgtattgcc cgcctggtgg agctcgtcga acagccgcat gatctcctcc
     4261 gaggtggcgg agtccaggtt gccggtgggc tcgtcggcca gcaggatgga cgggtcgttg
     4321 accagcgcgc gggcgatggc gacgcgctgc cgctggccgc cggacagctc gttgggctgg
     4381 tggtgcatgc gctgggcgag gccgacccgc tcgagcgccc ggcgcgcccg ctcccgccgc
     4441 tcgctgcgcg acaggccggc gtagaccagc ggcagctcga cgttgtgcag cgcgtcggtg
     4501 cgcgccagca ggttgaaggt ctggaagacg aagccgatct ccttgttgcg gatgccggcc
     4561 agctgctggt cgtcgagctc ctgcaccgcc gtgccgttga gcacgtagct tcccgacgag
     4621 ggggtgtcga ggcagcccag caggttcatc agcgtcgact tgccgctgcc cgaagagccc
     4681 atgatcgcca cgtactcgcc gcgctcgacc gagaggtcga cgccgttcag ggcgcggacc
     4741 tgctcggcgc ccatgtcata gaccttgtga atgtcccgca gctcgatcag cgccatcagt
     4801 cctgctcccc ctggtcgtcc gttgccgcgt cctcgccgcg gttttccgcc cggtcgcgct
     4861 cgcggtcggc ccggcggtcc gccgcctcgg aggtcgtgct gaccgtcacc gcgtcgccgt
     4921 cccgcaggtc gcgcagcgtc cggtacggac cggtcacgac cctctcgccg ggcttgacgc
     4981 cggagcggat ctcgacgtgg gtctcgtcgg agaggccggt ctccacggtc cgctgccgcg
     5041 ccttgccgtc ctccaggaca aacacgactt tgatctcgtc gtcttcctcc gccttcgcct
     5101 cctcgccctc ggccgtcaga ggggggcgct ccaccaccgc ctggatgggg agaaccaggg
     5161 cgttggcgtg ggcggcggtc tggatctcgg cccgcaccga catgccgggc cgcagcgcct
     5221 cgtcgctgtc ggtgagcagg accttgacct cgtagaaggt gacgtccggc tgctggggcc
     5281 ggttgaagcc cgagctgccg atctccacta cccgaccgtg gtagacccgg ttgggcaggg
     5341 cgtcgacccg cagcaccgcc tcctgcccca tccggacgtt cacgatctcg gtctcgtcca
     5401 cgtccacgtt ggccaggatc tcggacaggt cggcgatggt gccgatgatc gagccggcgt
     5461 tgttcatggt gccggagacc accacctccc cctgttcggc gttgagggcg atgacccggc
     5521 cggtcaaggg ggcgaagatg gtcgtcttgc tgaggtcgtc ctgcgccttc tcgagattcg
     5581 cctgcatctg gaggaccgac tcgcgggcct cgtccacacg cagccgggcg gaggtctcgg
     5641 cgagctcggc ggcttcgagc tgctcgttgg aggtgatgcc ctcctgagta aggcgccgcg
     5701 cccgctccag cttgtggcgg gcgtcggcca gcgacacctc ggcctgccgc acaccggtct
     5761 gggcactgcg gagctgcgcc gcccactggt tgcgctgggc gaggaacgcc tcccgctcga
     5821 gttgcaggaa cggctgcccc gcctggatca ggtccccctc ctccacgtac agcttctcga
     5881 tcttgccgat gacgtgggcc gagatgttga ccttgatccg gggatcgatc tcgccgctcg
     5941 ccttgacgat ctgggtcagg tcgcgccgcg ccgcctcctc ggcatagacc ctctccccct
     6001 tctcccgccc ggcgttcttc aggctggcga agacgatggc gccgagcagc agcaggacgc
     6061 cgacgatgac caggatcttc ttcacgagcc tgctccgccc gccatcgaac cgatcgccgc
     6121 cgcgaacccg gccttaccca ggacgtagag gagccagagg gcgatcaccg tgatcgccgc
     6181 cgccttgggg gagaccttcg ccaccacccg gtagccgatg acgaggagga tgagcgacca
     6241 gatcgagaag acgtccaggc tccccagcaa cgacttgacg accgggctcg cctcctccgg
     6301 cgccacggtc gcggcgctag aggtgaggat gccgccgctc atcgcctcct ccggagtcac
     6361 ggtgctccgc cccaggagga tcaggatgga gagcagcaga gagatggcgg acggcatcag
     6421 gccgtggacc gaggtcgcca ggctggactt gaaatccatc tccgagccga ccatcttgaa
     6481 ggcgacccag aagacgaggg cgacgatcag gaagaccacc gccgtcatga tggggccgac
     6541 gagcagcgtc accggcccca tcttctcgtt ccgctccacc atctcctgga tctggtcgtc
     6601 ggagacgttc cccatgccga actgcgacac ctggtgccgg accagctcct cctggtcgac
     6661 gcgctgctgc atcatgtagg cggccgtcgt ccccagcacc aggaggacga gcaggggggc
     6721 gagccaggtc ggccgctcgg cgatcgaccg gaacgtcttg cccggcgcca cgaggacgcc
     6781 gagcagacgg ccgaagctgg agtcgtgcat cgcggtctcc ttggtctaga acagcgtgaa
     6841 gcgattgatg ggctgctccg gatcgaccag ctccaccccc tgctgctcga gcagcgtgcc
     6901 ggtcgcccgg tagtactcgg tcaacgccgt ctggtacgcg atggtggcgt tgacctcgcg
     6961 gctgcgggcg agggtcaggt cttcctggat ctgggtgatc tcgaagctgg tcgacatgcc
     7021 gttctcatag cgcttgcgct cagcctccag attccgctcc tggaagcgga cggaggcgcg
     7081 ggcggcctcg atctgcttgg ccgcggtgtc gacccggcgc gccgcctgcc ggacctcggt
     7141 gcggatccgg tcctgcaggt tttcgagctc ggtctccgac cgttcgacgc ccaggttggc
     7201 gctggcgctc tgcgcccggg cggagcggtt ctggagcgga taggcaaaga cgagcgaggt
     7261 cgcccagccg gtgaggtcga ggccgaagag atcgctgtag acgtcgctga gcgcctcctg
     7321 actgccggcc gaagagccgt agctcaccga caggtcgagc cgcggccgtg tctggtttcg
     7381 gaaaaaggcg gcgttgatct ggttctgctc gagctggaac tgctgctgct ggagctcggg
     7441 gcgctcggcg aaggcggtgc tcaccgcctc ctcgacgttg aaggtgaggc gctccgcgct
     7501 ctccgggctg gtgacggcgt tgatcggcac ctcccagagc tcgccgggcg gcaggttgag
     7561 caggcgccgg agctcgtccg cggcgtcgcc cagggtggcc tgagcgctga tgatgtcctc
     7621 gtcgcgctcg gcgatcgccg cttcgctctg caccagctcc aggggggcca gggtccctac
     7681 ctccacctgg atccggttgc gctcgtggaa ctcgcgggcc agcgccatgc tctcttcggc
     7741 gacggcgagt tgctcccggg cgcccaccag gtcccagtag gcgttgacca cctgctggat
     7801 ggtcaaggtc acctgccgct cgaactcctc gcggctgatg cggttgtcga gctgcgccag
     7861 catcaggccg cgctcggtgg cgagacggcc gaagttgcgc agcagcggct gggtatagcc
     7921 gaaggtgagg ccgccgccat aggtgacggg cacgtcgcgg aaggtccccg ggcggaagct
     7981 gaagctgctc cgccgctggt ccacgccgat cgagaagtca cccccccagg gggcgagctg
     8041 ggaaaatccg aagtccaggg actgctcttc cgagctgacc ggcagcgtgg ggttctcggg
     8101 gtccgactcg tcttcgctgg ccgagagcgt cgcgtcggtc cgcaggtcgt agatgcccag
     8161 ggcctgctgg atggccaggc gctgctgctc acggacgtag agctggctgg ccaggtccag
     8221 gttctggcgc agcgccacgg cgatggcctg ctcaagggag agctggatcg ccccgttgcc
     8281 gacctccacc agcggctggc cgacgagcgg ctccggcggc ggcgtggtct ggagcacggt
     8341 cggcgccggc accgcgggcg gcgtctgggt gggcgtctga ctctgggtcg tcgtctgcgt
     8401 ctgcgtcgtc gtctgcggag tcgtggtctg ggcggaggcg ccaggtgcga ccagcagccc
     8461 tcccaccatg gaagcggtca cgaggacggc cgtcagcctc cgggccggcc gcacgtcccc
     8521 cgtatggaac cacaaagacg ttctcgtcat cagaatgcac ctcaaataag agaatcgttc
     8581 acactcagag ctggacgggt ccccggcgcg gacttccctc tcgagccgat ccttcccatg
     8641 ctcgatgtac gcgggacctc ctacaaagtt tacgtcggac cggccccgcc gtggcggcgc
     8701 cggccgggat cgggcaccgg caccgccgcc agcaggccgg cggtataggg atggcgcggg
     8761 cgggcgaaca cctcctcccg cggccccacc tccaccagcc ggccgcgatg gagcaccgcc
     8821 acccggtccg ccacctgctc caccgccgcc agatcgtggg cgatgaggag cagggtgagc
     8881 ccgagccgct cctggaggcc ggccaggagg ttgaggatct gggcgcgcac cgagacgtcc
     8941 agtgccgaga tgggctcgt
//
LOCUS       DASPJI010000660         9598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_212373, whole genome shotgun sequence.
ACCESSION   DASPJI010000660 DASPJI010000000
VERSION     DASPJI010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9598)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212373"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1604)
                     /locus_tag="VEL74_18675"
     CDS             complement(<1..1604)
                     /locus_tag="VEL74_18675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HYG64611.1"
                     /translation="MRRQASTGVVGSVHRGGRNEVEELRARLFEAEETLRALRDGEVD
                     ALVINEQRGERVYALKSAEHPYRKMVEGMRQGAATLASEGTILYCNPCLAEILRAPLG
                     SVVGCSVQRFLPEADRPAFEALLQEGLRGGSHGELVLRAEDGTTVPVYLAFSTLSLDE
                     LDAVCLVVIDLTEQKRNEQIVADEKLARSILEHATEAIVVCDLEGVILRASRTAQRLC
                     SGGCEGRLFSEAFPLRGRSGRPRGLEPHLRFCLEGRTSRGVEAAMSLPDGGERQLLLS
                     AGPLYSPHHEILGCVLTLTDITERRRAELELERARQEAEAANSAKDQFLASLSHELRT
                     PLTPVLAVLSGLGSDERLPDDVLDRLSMMRRNVELEARLIDDILDLTRIVRGKLELRR
                     EVTDLRTVLEHALQTCAAGMVAKRQWLVKELEPGDLYVWADTARLTQVFWNLLSNAVK
                     FTPEGGSIAMRSRTSEPAERSQLVVEVADSGIGIETEQLAHIFDAFHQGRVGITRRFG
                     GLGLGLAITRAIVELHGGTITAESAGR"
     gene            complement(1611..1922)
                     /locus_tag="VEL74_18680"
     CDS             complement(1611..1922)
                     /locus_tag="VEL74_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HYG64612.1"
                     /translation="MNADNPGHSERPEFWDLRLYVAGQTPKSVTALANLRRMCEEHLA
                     GRYRIEVVDLAENPQLARSDQILAIPTLVRRLPSPIRRIIGDLSNSERVLVGLDLRPR
                     S"
     gene            complement(1919..3631)
                     /gene="kaiC"
                     /locus_tag="VEL74_18685"
     CDS             complement(1919..3631)
                     /gene="kaiC"
                     /locus_tag="VEL74_18685"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HYG64613.1"
                     /translation="MARGIRKAPTGIAGLDEITFGGLPAGRPTLICGTAGCGKTLMAM
                     EFLVRGATGYGEPGVFVSFEERSEDLTENVLSLGFDLDELARQGKFLIEYIHVERSEI
                     QETGEYDLEALFLRLGWALDSIGGKRIVLDTLETLFSGFTNEGILRAELRRLFRWLKD
                     RGVTAVITAERGEGALTRHGLEEYISDCVILLDHRVDDQLATRRLRVVKYRGTHHGTN
                     EYPFLIDEQGIEVLPITSLRMEHGAPTERVSSGIAGIDEMLGGQGYYRGSSILVSGKA
                     GTGKTSIAAHFVDAACRRGERAVIFAFEEAFPQVARNMRSIGLGLERWTREGLLHFHA
                     ARPTLHGLERHLVTMYKLIRDVRPSVVVVDPITNFLTVGNPEEVKGMLMRLIDFFKLN
                     QITALFTSLTHSSYLEQTEVGTSSLMDTWLLVREVELSGERNRTINVIKARGMAHSNQ
                     LREMLFTEHGIQLTDVYLGPAGALTGAARAAQEAQERAAAIARQQETERRRRELERRR
                     QALEARIAALQLEFEVEQEEALRLIDQEGRREEQLQVDRAEMARVRRLMNSGSSGAGD
                     GGRE"
     gene            3844..4863
                     /gene="hemE"
                     /locus_tag="VEL74_18690"
     CDS             3844..4863
                     /gene="hemE"
                     /locus_tag="VEL74_18690"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018283363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HYG64614.1"
                     /translation="MSNLLFLRACRNEPTERPPVWLMRQAGRYLPEYREVRRKADFLT
                     LCKTPELAAEVTLQPIRILGVDAAILFADILLPLEAMGAELVFAAGDGPTFPKPVRRR
                     EDIQELLQPDVEKTLGYVFEAMRLVRRELDGQVAVLGFGGTPWTLAAYLVEGGGSKQF
                     AHLLAWSYEDPEGLALLLDRIADTSIAYLNGQIEAGAQAIQLFDTWGGLLDLERWRIL
                     ALPPLLKIVNALRGQVPLIYYVNGGGHLLPGMADLPVDILSVDWRQPLSAVRRVVGSR
                     VLQGNLDPAALLGPVPEIERRVARLIEEGKGGGHIVNLGHGILPMTPVEHARAFVAAV
                     QRSGG"
     gene            complement(4923..5903)
                     /locus_tag="VEL74_18695"
     CDS             complement(4923..5903)
                     /locus_tag="VEL74_18695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64615.1"
                     /translation="MRMSIRAAAVVAPMLALAAGVPVSADNVVRKGVDAWTTVKGFTQ
                     TSFAKDPIPAGFFCEGSQPFTGTIVMKGAPLATKPAQGLGEPADTVVARLDDAVFNAK
                     GEATTRIQLKALSLAGLEPIETGCGLYDVSVRLDGEQPVTTMKIVKTSAKGGSYEAPL
                     ALNVKVVFTPVTGDVKGVRELSRRVNLGPGTHSVWTFAERPRYEGSIWIDSDGNGKPD
                     MQVRGSNFVAGISPGKGDVRFATYSTYSSQPACPPGYCAKQSCHCNPNDSAWDPDMNR
                     DGCSDEHLHCVWVCVAAPGGSASSPSPGPGLQEDFACAFAEDIDEVESDL"
     gene            complement(6003..6587)
                     /locus_tag="VEL74_18700"
     CDS             complement(6003..6587)
                     /locus_tag="VEL74_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64616.1"
                     /translation="MRTSTSRPRSGLAVLHFLGLLLGLGACASPGGQPGGGGRFPLWV
                     EPVKQISLRRGHLGWGGVEVGMSRREAERAVGRRLPPPAELRDRPCGREVVEVDLMRQ
                     RLALEFDTGGEGRVAAIRLLMRNPRDPAGESGTIDLVRALKARFPGAVYAPLPAAGPV
                     PEALDPKPLYRLPEGGFALVEPIVGLVFGDVCTG"
     gene            complement(6571..7521)
                     /locus_tag="VEL74_18705"
     CDS             complement(6571..7521)
                     /locus_tag="VEL74_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYG64617.1"
                     /translation="MKGKTPAARLLALAAVLIALPAAAQRDFSKVEIKATKVAGKIYM
                     LEGAGGNIGVSVGSDGLLIVDDQFAPMADKIRAALKGLGEGKLKFVLNTHWHGDHTGG
                     NQAFGPEAPIIAHDNVRKRLATEQTVLGETTPPSPPEALPVITFDESLSVHFNGEEIR
                     VIHFPNGHTDGDSVIFFTGSNVVHMGDDFFHNRFPFVDLASGGSVLGLTRNIKAVLGR
                     LPADVKIIPGHGPLSTKADLERYHRMLVETTAIVSQRVHAGKTLEQIQAEGLPAEWKD
                     WGSGFIDANRWLSIVHQSLAKGMPEAPAHQHGPGTGAHKH"
     gene            complement(7540..8169)
                     /locus_tag="VEL74_18710"
     CDS             complement(7540..8169)
                     /locus_tag="VEL74_18710"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HYG64618.1"
                     /translation="MPFLLTPETEEVDMRPVRRLRRAAGFTLIEALVVLAILFVLLTL
                     GLPELLRVVHRSKLEGMAQGVSGLMRSARYEAVKQNVNTRVIVDYGNDRVIAATVDGA
                     GDPIQQLGAQPLPDTIDFWAWNEDPRGANAIDGFTTTATGGWVEFRPNGSADTVGAIR
                     LADGRNFLEVAVATQATGRIELRKAEPDPVTSAIRYRLNGERENAWAWK"
     gene            complement(8250..9245)
                     /locus_tag="VEL74_18715"
     CDS             complement(8250..9245)
                     /locus_tag="VEL74_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYG64619.1"
                     /translation="MRLLVLGGTRFVGRHLVEAALTRGHQVTLFNRGRSHPGLFPEAE
                     ELHGDRMSDLSALDGRRWDAVLDVNGYVPRSVRASAGKLAGAVDLYVFISTISVYAHL
                     EEPGVSEESPVQEPPPGGEDAEEVTNESYGWLKALCERAAEEAMPGRVLHVRPGIQSG
                     PHDYTGRFAWWVTRVALGGEVLAPGRPESPIQAVDARDLATWVVRMTEERATGVYNVT
                     GPEPPLTMEGMLAACREASGSDAVFTWVGDTFLAEHGIELPLWLTCEEEGFGRVISAR
                     ARARGLTLRPVEETARDVLAWVAEDPEHRTPERLAPAREAGLLREWHRQRRGGQG"
     gene            complement(9253..>9598)
                     /locus_tag="VEL74_18720"
     CDS             complement(9253..>9598)
                     /locus_tag="VEL74_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG64620.1"
                     /translation="IEDHRDAADSLQILLELAGHEVEVAYDGPQGVEKARRFRPEIVL
                     CDIGLPDGMDGYAVARALRTDPEVGALRLVALSGYGQAEDQRRALEAGFDAHLTKPAD
                     PDGLRRLLAGLP"
BASE COUNT         1449 a         3322 c         3331 g         1496 t
ORIGIN      
        1 cgccccgcgc tctccgcagt gatggtgccg ccgtgcagct cgacgatggc acgagtgatg
       61 gcgaggccca gtccgagccc tccgaagcgg cgggtgatgc cgacccgtcc ctggtggaag
      121 gcgtcgaaga tgtgcgccag ctgctccgtc tcgatgccga tccccgagtc ggcgacctcc
      181 accacgagct ggctccgctc cgcgggctct gatgttcggg accgcatggc gatggagccg
      241 ccctcgggcg tgaacttgac cgcgttgctc aagaggttcc agaacacctg ggtgaggcgc
      301 gcggtgtcgg cccagacgta caggtctccg ggctccagct ccttgaccag ccactggcgc
      361 ttggcgacca taccggcggc gcaggtctgg agggcgtgct ccagcaccgt ccgcagatca
      421 gtgacttcgc ggcgcagctc gagcttgccg cggacgatgc gggtcaggtc gaggatgtcg
      481 tcgatgagtc gcgcctcgag ctccacgttg cggcgcatca tcgacaggcg gtcgagcacg
      541 tcatcgggga gccgctcgtc ggagccgagc cccgacagca ccgccagaac gggggtcagg
      601 ggagttcgca gctcatggga gaggctggcc aggaactggt ccttggcgct gttggcggcc
      661 tccgcttcct ggcgggcgcg ctccaattcc agctctgccc ggcggcgctc ggtgatatcg
      721 gtgagggtca ggacacagcc gaggatctcg tggtgcggcg agtagagggg acccgcagag
      781 agcaggagct gccgctcgcc gccgtccggc agagacatgg ccgcttcgac accgcggctg
      841 gtccgccctt ccaggcagaa acggaggtgg ggctcgaggc cgcgcgggcg tccggaccgg
      901 ccgcgcaagg gaaaggcttc agagaagagc cggccctcgc agccgccgct gcagagccgc
      961 tgggctgtcc tgctggcacg caggatcacc ccttccagat cgcaaaccac gatggcctcg
     1021 gtggcgtgct cgaggatgga acgggccagc ttctcgtcgg cgacgatctg ctcgttgcgc
     1081 ttctgctcgg tcaggtcgat cacgaccagg cagacggcat cgagctcgtc gagggaaagg
     1141 gtgctgaagg ccaggtagac gggcaccgta gtgccgtcct cggcgcgcag gaccagctcc
     1201 ccgtggctgc cgccgcgcag accttcctgc agcagggctt cgaaggcagg ccggtccgct
     1261 tcaggcagga aacgctgaac cgagcagccg actacgctcc ccagcggcgc ccgcaggatc
     1321 tcggcgaggc atgggttgca atagaggata gtcccctcgg aggccagggt ggcggccccc
     1381 tgccgcatcc cctccaccat cttgcggtag gggtgctcgg cactcttgag ggcatagacc
     1441 cgctcgccgc gctgctcgtt gatgacgagg gcgtccacct cgccgtcgcg cagggcgcgg
     1501 agggtctcct ccgcctcgaa gagccgcgcc cgcagctcct ccacctcgtt acggccgccg
     1561 cggtggacgc tcccgacgac accggtggac gcttgccggc gcatctgcca tcagctccgg
     1621 ggacggaggt ccagccccac cagcacgcgc tccgagttgg agaggtcgcc gatgatcctg
     1681 cggatcggcg acgggagacg ccggacgagg gtcgggatgg ccaggatctg gtcgctccgg
     1741 gcgagttgcg ggttctctgc gagatcgacc acctcgatgc ggtacctgcc agccaagtgc
     1801 tcctcgcaca tccgccgcag attcgccagg gcggtcaccg acttgggcgt ctgccccgcc
     1861 acgtagagcc gcaggtccca gaactccggg cgctcgctgt ggccggggtt gtccgcgttc
     1921 attcccgtcc tccatccccg gcgccggagg aaccggaatt catcagccgg cggacgcgcg
     1981 ccatctcggc acggtcgact tgtagctgct cttcccgccg gccctcctgg tcgatgagcc
     2041 gcagggcctc ttcctgctcc acctcgaact cgagctgcag cgcggcgatg cgggcctcca
     2101 aggcctggcg ccggcgctcc agctcgcgcc gccggcgctc ggtctcctgc tggcgggcga
     2161 tcgccgccgc ccgctcctgc gcctcctggg ccgcccgggc cgcgccggtg agagcgccgg
     2221 ccgggccgag atagacgtcg gtgagctgga tcccatgttc ggtgaacagc atctcccgga
     2281 gctggttcga atgcgccatg ccgcgggcct tgatgacgtt gatcgtccgg ttgcgctcgc
     2341 ccgagagctc gacctcgcgc accagcaacc aggtgtccat gagagacgag gtgccgacct
     2401 cggtctgctc caggtaggag gagtgggtga ggctggtgaa gagggcggtg atctggttca
     2461 gcttgaagaa gtcgatcagg cgcatgagca tgcccttgac ttcctcgggg ttccccacgg
     2521 tgaggaagtt ggtgatcggg tccaccacca ccaccgaagg gcgaacgtcg cggatcaact
     2581 tgtacatggt gaccagatgc cgctccaggc cgtgaagggt cgggcgggcg gcatggaagt
     2641 gcagcagccc ctcccgcgtc cagcgctcca ggccgaggcc gatggagcgc atattgcggg
     2701 ccacctgggg gaacgcctcc tcgaaggcga agatcaccgc ccgctcgcca cgccggcagg
     2761 ccgcgtccac gaagtgggcg gcgatgctcg tcttgcccgt gcccgcctta cccgagacca
     2821 ggatgctgct cccccggtag tacccctggc cgccgagcat ctcgtcgatg ccggcgatgc
     2881 cgctggacac ccgctcggtg ggcgccccgt gctccatccg cagtgaggtg atgggcagaa
     2941 cctcgattcc ctgctcgtcg atgaggaacg ggtactcgtt ggtgccgtgg tgggtgcccc
     3001 ggtacttgac cacccgcagc cgccgcgtcg cgagctggtc gtccacccgg tggtcgagga
     3061 ggatgacgca gtcggagatg tactcctcga gaccgtggcg ggtgagcgcc ccctccccgc
     3121 gctcggcggt gatcaccgcg gtcaccccac gatccttgag ccagcggaaa aggcgccgca
     3181 gctcggcgcg caggataccc tcgttggtga agccgctgaa tagggtctcc agggtatcga
     3241 ggacgatccg cttgccaccg atcgagtcca gggcccatcc gaggcggagg aagagcgctt
     3301 ccaggtcgta ctcgcccgtc tcctggatct cgctgcgctc cacgtggatg tactcgatga
     3361 ggaacttgcc ctggcgcgcc agctcgtcca gatcgaaccc cagcgacagg acgttctccg
     3421 tcaagtcctc cgagcgctcc tcgaagctga cgaagacccc gggttcacca tacccggtgg
     3481 cgccgcgcac caggaactcc atggccatca atgtcttgcc gcagccggcg gtgccgcaga
     3541 ttagggtcgg cctccccgcc ggaaggccac cgaaggtgat ctcgtccagg ccggcgatgc
     3601 cggtgggtgc cttgcggatc ccccgcgcca tggccggctc ggaggggggc cgactctcga
     3661 cgggagccgg agcagccgag gaggaaggaa ccgtctggaa attatctgtg tcgagcatga
     3721 tcgagcgatc ccgaagagca agcctcgtgc ccgccccttg gcgggccacc ggcggcccgt
     3781 ctgcgtcatc aggctgacct caatagtagt cgccctctcg ccctcctgct atcctccccc
     3841 gccgtgagca acctactctt cctgcgcgcc tgccgcaacg aacccacgga gcgaccgccg
     3901 gtgtggctca tgcgccaagc aggacgttac ctccccgagt accgtgaggt ccgccgcaag
     3961 gccgacttcc tgactctctg caagactccg gagctggcgg cggaggtgac gctgcagccg
     4021 atccgcattc tcggcgtgga cgccgctatc ctcttcgccg acatcctgct gcccctggaa
     4081 gccatgggcg ccgagctggt cttcgccgcc ggcgacggcc ccaccttccc caagccggta
     4141 cgccgccggg aggatatcca ggagctgctg cagccggacg tggagaagac tctggggtac
     4201 gtcttcgaag ccatgcgcct ggtgcgccga gagctcgatg gtcaggtggc cgtcctcggc
     4261 ttcggcggca ccccgtggac actggccgcc tatctggtcg agggcggggg ctccaagcaa
     4321 ttcgcccacc tgctcgcctg gagctacgag gacccggagg ggctcgccct gctgctcgac
     4381 cggatcgccg acacctccat cgcctacctg aacggccaga tcgaggcggg cgcgcaggcg
     4441 atccagctgt tcgacacctg gggcggcctg ctcgacctgg agcgctggcg gatcctcgcg
     4501 ctgccgccgc tcctcaagat cgtcaacgcc ttgcgcggcc aagtccccct catctactac
     4561 gtgaacggcg gtggccacct gcttccgggc atggcggacc tgccggtcga tatcttatcg
     4621 gtggactggc gacagccgct ctcggcggtg cgccgggtgg tcggctcccg ggtgctccag
     4681 ggcaacctgg acccggcggc gctcctcgga cccgtcccgg agatcgagcg gcgggtggcg
     4741 cggctgatcg aggagggcaa ggggggtggc cacatcgtca acctcggcca cggcatcctg
     4801 cccatgaccc cggtcgagca cgcccgggcg ttcgtggcgg cggtgcagcg ttccggcggt
     4861 taagttgagg gacgggcggc gccatccgcg gccggacggc gccacctaac gccagggaga
     4921 gatcagaggt cgctctcgac ctcgtcgata tcctcggcaa aggcgcaggc gaagtcttcc
     4981 tgcaggccgg gcccggggga tggcgagctc gccgatccgc cgggagccgc cacgcagacc
     5041 cacacgcagt ggaggtgctc gtcgctgcag ccgtcgcggt tcatatccgg atcccatgcg
     5101 gaatcgttgg gattgcagtg gcacgactgc ttggcgcagt agcccggagg acaggccggc
     5161 tggctgctat aggtgctgta ggtggcgaag cggacgtcac ccttgcccgg tgatatgccg
     5221 gcgacgaagt tcgagccgcg cacctgcata tccggcttgc cgttgccgtc ggagtcgatc
     5281 cagatcgacc cttcgtagcg cggccgctcg gcgaaggtcc agaccgagtg ggtgccgggg
     5341 ccgaggttga cccgccgtga cagctcgcgg acgcccttga cgtccccggt gaccggcgtg
     5401 aacaccacct tgacgttcag ggcgaggggc gcctcgtagc tgccaccctt ggcgctggtc
     5461 ttgacgatct tcatggtggt gacgggctgc tcgccatcca ggcggacgga cacgtcgtac
     5521 agcccgcagc ccgtctcgat gggctcgagg ccggccagcg acagcgcctt gagctggata
     5581 cgggtcgtcg cctctccctt ggcattgaac accgcgtcat ccaggcgggc gaccacggtg
     5641 tcggccggct cgcccagccc ctgcgccggc ttggtggcga gcggcgcccc cttcatcacg
     5701 atggtgccgg tgaagggctg cgagccctcg cagaagaagc ccgccgggat cggatccttg
     5761 gcgaagctcg tctgggtgaa acccttgacc gtggtccagg cgtccacacc cttgcggacc
     5821 acgttgtccg ccgagacggg cacgcccgcg gccagagcca gcatcggagc cactacggca
     5881 gcagcacgga tcgacatcct cattctgctc tcctttctcc acattcagga cattcaggca
     5941 ccccggacgc gggtgcctct attgaaggga gtgcacggag agagggaggg cgagcctccg
     6001 gatcagccgg tgcagacgtc gccgaaaacg aggcccacga tgggttcgac caaggcaaag
     6061 cccccttccg gcaggcgata gaggggcttg gggtcaagag cctcgggtac cgggccggcg
     6121 gcgggaaggg gtgcgtagac tgcgccggga aagcgggcct tgagagcgcg gacgaggtcg
     6181 atcgtccccg attctcccgc ggggtcgcga gggttgcgca tcagcagccg gatggcggcg
     6241 accctgccct ctcctccggt gtcgaattcc aaggcgagcc gctgccgcat gagatcgacc
     6301 tccaccacct cgcggccaca cgggcggtcg cgcagctcgg ccgggggcgg cagccgccgc
     6361 cccacggcgc gctcggcttc gcgccggctc atgccgacct cgacgccgcc ccagcccagg
     6421 tggccccggc gcaggctgat ctgcttgacc ggctcgaccc agagggggaa gcgcccgcct
     6481 ccccccggct ggccgcccgg cgaagcacag gcgccgagcc cgagcaggag cccgagaaag
     6541 tggagtacgg cgagaccgga gcgcggccgg ctagtgcttg tgcgcaccgg tcccggggcc
     6601 gtgctggtgc gccggcgcct ccggcatccc cttggccagg ctctggtgga cgatggagag
     6661 ccaccggttg gcgtcgatga agccgctccc ccagtccttc cactccgcgg gtagcccctc
     6721 ggcctggatc tgctcaaggg tcttgccagc atggacgcgc tggctgacga tggcggtggt
     6781 ctcgaccagc atgcggtgat agcgctcgag gtcggccttg gtggacagcg gcccgtgacc
     6841 ggggatgatc ttcacgtcgg caggaaggcg cccgaggacc gccttgatgt tgcgggtgag
     6901 ccccaggacg ctgccgccgc tcgccaggtc cacaaagggg aagcggttgt ggaagaagtc
     6961 gtcccccatg tgcaccacgt tcgagccggt gaagaagatg acgctgtcgc cgtcggtgtg
     7021 gccgttgggg aagtggatga cccggatctc ctcgccgttg aagtggacgg agagggattc
     7081 gtcgaaggtg atcaccggca gcgcctcggg cggcgagggg ggcgtcgtct cccccagaac
     7141 ggtctgctcg gtggccagac gcttccgcac gttgtcgtgg gcgatgatcg gcgcctcggg
     7201 gccgaaggcc tggttaccac cggtgtggtc gccgtgccag tgggtgttga gcacgaactt
     7261 gagctttccc tcacccagcc ccttgagggc ggcgcggatc ttgtccgcca tcggggcgaa
     7321 ctggtcgtcg acgatcagca ggccgtcgct ccccaccgac actccgatgt tgccgcccgc
     7381 cccctccagc atgtagatct tgccggcgac cttggtcgcc ttgatctcca ccttggagaa
     7441 gtcgcgctgc gcggcggccg gcagcgcgat gaggaccgcg gcgagggcca gcaggcgggc
     7501 ggccggggtc tttcccttca tgaagtctcc tctcgctggt tatttccagg cccaggcgtt
     7561 ctcgcgctca ccattgagcc gatagcggat ggcgctggtc acggggtccg gctcagcctt
     7621 gcgcagctcg atgcggcccg tggcctgggt ggccaccgcc acctcgagga agttgcggcc
     7681 atcggccagc cgtatggctc ccaccgtgtc ggccgagccg ttgggccgga actccaccca
     7741 gccgcctgtc gccgtggtgg tgaagccgtc gatcgcgttg gcccctcgcg gatcctcgtt
     7801 ccaggcccag aaatcgatgg tgtcggggag cggttgcgcg ccgagttgct gaatggggtc
     7861 gccggcgcca tccacggtgg cggcgatgac ccgatcgttt ccgtagtcca cgatcacgcg
     7921 cgtattgacg ttctgcttga ccgcctcgta gcgggcggac cgcatcaggc ccgacactcc
     7981 ctgggccatc ccttcgagct tactgcggtg cacgacccgc agcagctccg gcaggccgag
     8041 cgtcaacaga acgaacaaga tggccagaac gaccaacgcc tcgatcagcg tgaatccagc
     8101 cgcgcggcgg agcctccgaa cagggcgcat atcgacctcc tccgtctccg gcgtcagcag
     8161 gaaaggcaac ccgcctcccc cggctctccg gcccggtact acttagatga atcgaaatga
     8221 tagcacggga ggaataaccg ggaggtcgct cacccctgcc cccctcggcg ctgccggtgc
     8281 cactcgcgca gcaggccggc ctcgcgcgcc ggagcgaggc gttcgggagt acggtgctcc
     8341 gggtcctcgg ccacccaggc gaggacgtcg cgggcggtct cctcgaccgg acggagggtg
     8401 agcccccggg cgcgcgcccg ggccgagatc acccggccga agccctcctc ttcgcaggtc
     8461 agccagagcg gcagctcgat cccgtgctcc gcgaggaagg tgtcgcccac ccaggtgaac
     8521 accgcgtcgc tgccggaggc ctcgcggcag gccgccagca tcccctccat ggtgagcggg
     8581 ggctcgggac cggtgacgtt gtagacgccg gtggcccgct cctcggtcat ccgcaccacc
     8641 cacgtggcga gatcgcgggc atccaccgct tggatggggc tctccggccg gccgggagcc
     8701 agcacctctc caccaagggc gacacgggtg acccaccagg cgaagcggcc ggtgtagtcg
     8761 tgcggccccg actggatgcc ggggcggacg tgcagcacgc ggccgggcat cgcctcctcc
     8821 gccgcccgct cgcagagggc cttgagccag ccgtagctct cgttggtgac ctcctcggcg
     8881 tcctccccgc ccggcggcgg ctcctggacc gggctctcct cgctgacgcc gggctcctcc
     8941 agatgggcat agaccgagat ggtggagatg aagacataga ggtctacggc gccggcgagc
     9001 ttgccggccg acgcgcggac gctccgcggc acatagccgt tgacgtccag caccgcgtcc
     9061 cagcggcggc cgtccagagc ggagagatcc gacatccggt caccgtggag ctcttcggcc
     9121 tcggggaaga gcccgggatg gctccggccg cggttgaaga gggtcacctg atggccgcgg
     9181 gtgagcgcgg cttccaccag atgccggccg acgaaacgcg ttccaccgag gaccaggagc
     9241 ctcatgggac ctctacggca ggccggcgag caggcggcgc aggccgtcgg gatcggcggg
     9301 cttggtgagg tgggcgtcga agccggcctc cagggcgcgg cgctggtcct cggcctggcc
     9361 gtagccggag agggccacga ggcggagggc gccgacctcc ggatcggtgc gcagagcccg
     9421 ggccacggcg tagccgtcca tgccgtccgg caggccgatg tcgcacagca cgatctccgg
     9481 ccggaaccgc cgcgccttct ccaccccctg cgggccatcg taagccacct cgacctcgtg
     9541 accggccagc tcgaggagga tctgcaggct gtcggcggcg tcccggtggt cctcgatg
//
LOCUS       DASPJI010000661         4621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_788377, whole genome shotgun sequence.
ACCESSION   DASPJI010000661 DASPJI010000000
VERSION     DASPJI010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788377"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VEL74_18725"
     CDS             <1..640
                     /locus_tag="VEL74_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HYG64621.1"
                     /translation="NEEYESALRAFIEALLDDKELVADLEAFVEPLVEPGRIHSLAQT
                     LLKLTTPGVPDHYQGTEVWDLSLVDPDNRRPVDYDLRRRLLEDLKKDGITPEEILART
                     DDGLPKMWVIRQALRLRRSRPDAFGDTGTYRPLPAGGPKADHTVAFTRGDEVAVVVPR
                     LVLRLDGDWGDTSLDPPPGRWHNELTGEEVEGGRRRVADLLARFPVALLSRA"
     gene            complement(637..1059)
                     /locus_tag="VEL74_18730"
     CDS             complement(637..1059)
                     /locus_tag="VEL74_18730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64622.1"
                     /translation="MKDHRDLPAFHSTHELLLAVHQATNRLPTAGEPGGIAIRLRATT
                     LSAAGAVVRGCGLTGRRFAGELEHAACRLREIGYYIDVAQSLGYLTLESALDLLEKQA
                     RARLEVTALLQSIAEDEVAGHVILGSMRAAPEVVQIQL"
     gene            complement(1139..1714)
                     /locus_tag="VEL74_18735"
     CDS             complement(1139..1714)
                     /locus_tag="VEL74_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64623.1"
                     /translation="MRHTLLRTLPFANVVLALVAIVLGGCHAEEPERNVSAGAAEQPP
                     PETRDRKAPSPRAELVATGRQSFRGTPRYHCVLHDESGVQINLRTGDPGLPVVTVRLD
                     DYQGTGSYRARLFVTGRSGTGALVRSLGVAELELRGSQPAGPAETTLLSGNFEGTYGG
                     EAGQGSVEGHFSGCSYRQVRGGPPVLADSNH"
     gene            complement(1723..2247)
                     /locus_tag="VEL74_18740"
     CDS             complement(1723..2247)
                     /locus_tag="VEL74_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64624.1"
                     /translation="MDDLQRSILFRSVVSMLLLVALVTLTGCESEVEDATTPAEAAEE
                     AQGAGHLRLTGSQNVDGQYTVGCELTPDKGLVFTFTQLGTNPGPQVEVRIGDYHAAGS
                     YQGPMVVRTRSGQDQAVESNGTARIEVRSQEDRGVRGTTELSGSFEGAYQGAAGQGNA
                     TGHIERCTYKNLVR"
     gene            2356..2712
                     /locus_tag="VEL74_18745"
     CDS             2356..2712
                     /locus_tag="VEL74_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64625.1"
                     /translation="MRRVFVLMGVVGLLVWALMPGLPASASQMCSTSCGATTIQCCVS
                     TGTCTSSAGSLNCNGTVYTCDAINAYNACKLDCLDRYMTCRSFCTDRTCLSECAEFRT
                     WCIADCGPMPQTSFGC"
     gene            complement(2758..4500)
                     /locus_tag="VEL74_18750"
     CDS             complement(2758..4500)
                     /locus_tag="VEL74_18750"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HYG64626.1"
                     /translation="MTRGSECGIVGPVNGRIDFQRYRKLRAFVTRTFLHALWWDILLA
                     GPVVRRFRRPPAERWTRIAKRYRALAVEMGGVLIKLGQFLSVRVDLLPAEVTRELSGL
                     QDEVPAERFEDVARQIEADFGRPLTAVFSWVDPVPVGAASLAQVHHARLPGGEPVVVK
                     ALRPGIDVLVETDLAAVSLALRLLKFSRNIRKRVDLDRLGIEIVHTTLRELDLANEGR
                     SAERFAADFADDPRVYVPKVFWQWSARRTLTLENVGFLKIGDLETLGRAGIPSAEVAR
                     VLYRLYMQQIFVHHFVHSDPHPGNLFVRALPLPGEAAFGPGDSVPPPPPGQERPFQIV
                     FIDFGMVAEIPDHLRAALREYVIGLGTRDPARVVHAYQSAGVLLPGADLTRLEEIHEE
                     LFDRFWGVSIGNLRDMALSEARYFLVQYRDLLYEIPFQVQVELLFAMRAVGLLAGLST
                     YLNPEFDPWAETMPFARRLAKEDLKRDWRGWLDEALFQGRLLLELPRRLDDVLVRAER
                     GGLTVQAALAQETKRTIQRLEKSLQKVAWMVISAALLLAGVMLHTDAPGEPYGSWLMG
                     AAGAAFLWATLGKR"
BASE COUNT          710 a         1613 c         1619 g          679 t
ORIGIN      
        1 caacgaggag tacgagtcgg ccctgcgcgc gttcatcgag gcgctcctgg acgacaagga
       61 gctggtggcg gatctggagg ccttcgtgga gccgctggtg gagccgggcc ggatccactc
      121 ccttgcccag acactcctca agctcaccac gcccggcgtc cccgaccatt accagggcac
      181 ggaggtctgg gatctctccc tggtggaccc ggacaaccgc cggcccgtgg actacgatct
      241 gcgccggcgg ctgctcgaag atctgaagaa ggacgggatc acccccgagg agatcctcgc
      301 ccggacggac gacgggctgc ccaagatgtg ggtgatccgc caggccctcc gcctgcgccg
      361 gagccgcccc gacgccttcg gggacaccgg cacctaccgg cccctgcccg ccggcggccc
      421 caaggcggac cacaccgtcg ccttcacccg gggggacgag gtggccgtcg tggtgccccg
      481 gctggtgctc cggctggacg gcgactgggg cgacacctcc ctcgaccccc cccccggccg
      541 ctggcacaac gagctgacgg gcgaagaggt cgagggcgga cgccgtcgcg tcgccgacct
      601 cctcgcccgc ttcccggtgg cgctgttgtc gcgcgcctag agctggatct gcacgacctc
      661 cggcgccgcg cgcatcgatc ccaggatcac gtgccccgcc acctcgtcct cggcgatgga
      721 ctggaggagg gcggtcactt cgagccgcgc ccgcgcctgc ttctccagca ggtcgagggc
      781 ggactccagg gtgaggtagc cgaggctctg ggccacatcg atgtagtagc cgatctcgcg
      841 cagacggcag gcggcgtgct ccagctcccc ggcgaagcgc cggccggtca agccgcagcc
      901 gcgcaccacg gcgccggcgg ccgacagcgt cgtcgcccgc aaccggatgg cgatgcctcc
      961 cggctctccc gcggtgggga ggcggttcgt cgcctgatgg acggccaaca gcagctcgtg
     1021 ggtggagtgg aaggcgggga ggtcgcggtg gtccttcatc gttcttctcc tctcgaaagt
     1081 gtccctgcgg acggttcgaa agggagaaga cgtattccgc ggaaaaaatc gctcaatttt
     1141 agtggttgct gtcggccagg accggtggac cgccccggac ctgccggtag gagcagcctg
     1201 agaagtgccc ctcgaccgag ccctggccgg cctcgccgcc gtacgttccc tcgaagttgc
     1261 cgctcagcag ggtggtctcg gcgggcccgg cgggctgcga gccgcgcagc tcgagctcgg
     1321 ccacgccgag ggagcggacc agggcgccgg tgccgctgcg gccggtgacg aacaggcggg
     1381 cgcggtagga gcccgtcccc tggtagtcgt ccaggcgcac cgtcaccacg ggcaggccgg
     1441 ggtcgccggt gcgcaggttg atctggacgc cgctctcgtc atgcaggacg cagtgatagc
     1501 gcggggtgcc cctgaacgac tgccggccgg tggccaccag ctcggcgcgg ggggatgggg
     1561 ccttccggtc acgggtctcc ggcggcggct gctcggccgc gccggcgctc acgttccgct
     1621 cgggctcctc ggcgtggcag ccgccgagga cgatggcgac gagcgccagg acaacgttcg
     1681 cgaaggggag cgtccggagc agggtatggc gcaccgtgcc tcctaccgta cgaggttctt
     1741 ataggtacag cgttcgatat ggcccgtggc gtttccctgt cccgccgccc cctggtaggc
     1801 cccttcgaac gagccggaca gctcagtcgt tcctcggacg cccctgtcct cctggctgcg
     1861 gacctcgatg cgggcggtgc cgttcgactc gacggcctga tcctgcccgg accgggtccg
     1921 taccaccatg gggccctggt acgagcccgc tgcgtggtag tccccgatgc gcacctcgac
     1981 ctgcggcccg gggttcgtcc cgagctgggt gaaggtgaag accagcccct tgtcgggggt
     2041 cagctcgcag cccaccgtgt attgaccgtc gacgttctgg ctgccggtca ggcgcaggtg
     2101 ccccgcgcct tgggcctcct cggccgcctc ggccggagtc gtcgcgtcct ccacttcgga
     2161 ctcgcacccc gtcagggtca cgagggccac cagcaggagc atggaaacga cactacggaa
     2221 caggatcgag cgctgcaagt catccatatg ccacacctcc tccggtttcg cggcggtgca
     2281 ggatggatgc cacatctagc ctatgatata agccgatcca tacttaagga tcactcaact
     2341 cgaaggagga gaacgatgag aagagtcttc gtcctgatgg gggtggtggg tctcctggtc
     2401 tgggcgctga tgccggggct gccggcaagc gcgtcgcaga tgtgcagcac gagctgtgga
     2461 gccacgacga tccagtgctg tgtctccacc ggcacctgca ccagctcggc cggcagcctc
     2521 aactgcaacg ggaccgtcta cacgtgtgac gcgatcaacg cctacaacgc ttgcaagctg
     2581 gactgcctcg atcgctacat gacgtgccgt agcttctgta cggaccgcac ctgcctgtcg
     2641 gagtgcgcgg agttccgtac ctggtgcatc gcagactgcg gtcccatgcc gcagaccagc
     2701 ttcggctgct gagcctgatc ctggcggagg gaggggctcc ggcccctccc cctccagtca
     2761 ccgcttcccc agagtcgccc acaggaacgc ggcccccgcc gcccccatca gccagctccc
     2821 gtagggctct cccggagcgt cggtgtggag catcacgccg gcgaggagca gcgccgccga
     2881 gatcaccatc caggccacct tctgcagcga cttctccagc cgctggatgg tgcgcttcgt
     2941 ctcctgcgcc agcgccgcct ggaccgtcag cccgccgcgc tccgcccgca ccaggacgtc
     3001 gtccagccgc cgcggcaact cgaggagcag ccggccctgg aagagggctt cgtcgagcca
     3061 gccgcgccag tcgcgcttca gatcctcctt ggcgaggcgg cgggcgaacg gcatggtctc
     3121 cgcccagggg tcgaactcgg gattcagata ggtcgacaac ccggcgagga ggcccaccgc
     3181 ccgcatggcg aagaggagct cgacctgcac ctggaacggg atctcgtaga ggaggtcccg
     3241 gtactgaacc aggaagtagc gggcctcgga cagcgccatg tctcgcaggt tgccgatgct
     3301 gaccccccag aagcggtcga acagctcctc gtggatctcc tccaggcggg tcaggtccgc
     3361 cccgggcagc agcacgccgg cgctctggta ggcgtgcacg acgcgggcgg ggtcgcgcgt
     3421 gcccaggccg atgacgtact cgcgcagggc ggcccgcagg tggtccggga tctcggccac
     3481 catgccgaag tcgatgaaca cgatctggaa gggccgctcc tggccgggag gcgggggagg
     3541 cacggagtcg cccggcccga aagccgcctc gccggggagc ggcagcgcgc ggacgaacag
     3601 gttcccgggg tgggggtcgg agtgcacgaa gtggtgcacg aagatctgct gcatgtagag
     3661 ccggtagagg acccgggcca cctcggcgct cgggatgccg gcccgcccca gggtctcgag
     3721 gtcgccgatc ttgaggaagc cgacgttttc cagggtcagc gtccggcgcg cgctccactg
     3781 ccagaagacc ttggggacgt agacccgggg atcgtccgcg aagtcggcgg cgaagcgttc
     3841 ggcgctccgg ccctcgttcg ccaggtccag ctcgcgcagg gtggtgtgga cgatctcgat
     3901 acccaaacgg tcgaggtcca cccgcttgcg gatgttgcgg gagaacttga gcaggcgcag
     3961 ggccagggag accgccgcga ggtccgtctc caccaagacg tcgatccccg gccggagcgc
     4021 cttgaccacc accggctcgc cgccgggcag gcgggcgtgg tggacctggg cgagcgaggc
     4081 ggcgcccacc ggtaccgggt cgacccagga aaagaccgcg gtcagcgggc gtccgaagtc
     4141 ggcctcgatc tggcgggcga cgtcctcgaa gcgctccgcc ggcacctcgt cctggaggcc
     4201 ggagagctcg cgggtcacct ccgcggggag gaggtccacc cgcacgctca ggaactggcc
     4261 gagcttgatg agcacgcccc ccatctcgac ggcgagggcg cggtagcgct tggctatccg
     4321 cgtccagcgc tcggccggag ggcgccggaa gcggcgcacc acggggccgg cgagcaggat
     4381 gtcccaccag agggcgtgca ggaaagtgcg ggtgacgaac gctcgcagct tgcggtagcg
     4441 ctggaagtcg atccgcccgt tcacggggcc cacgatgccg cactcggagc ccctggtcaa
     4501 ccatcgccct cttttgacaa gatcccccgc gccatgacct ccgacgtcac cttgcgtgag
     4561 ctggcgagcc acgaggacta cctggcctgc gtcgccctgc agaaggagac ctggggccag
     4621 g
//
LOCUS       DASPJI010000662        21867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_610274, whole genome shotgun sequence.
ACCESSION   DASPJI010000662 DASPJI010000000
VERSION     DASPJI010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 21867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21867)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21867
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610274"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VEL74_18755"
     CDS             complement(<1..380)
                     /locus_tag="VEL74_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64627.1"
                     /translation="MAYRRSSRPVPRRSGRARRSGVSGLWLFLLGLLVGAGGLYMAFR
                     DTPGDSGREVATATQEAEATEAAREGRLEPRATGASGASEAATSEGPDAPEGDQPPPE
                     ASTEPGDEGGDEAAPAGEGGKLGRV"
     gene            complement(445..1644)
                     /locus_tag="VEL74_18760"
     CDS             complement(445..1644)
                     /locus_tag="VEL74_18760"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYG64628.1"
                     /translation="MSRSRVIFFLVSAVLVFPILAGTLLLAADRQEPQEDSLYKYLAI
                     FSEVLGMIRQAHVDEPDMNALMAGALDGTTDALDPFSLYIPAGQVEGYLDARAVGRRH
                     TGMTLLKESGIAYVVTVEKGSPAGLAGVQPGDLISKVDGRSTRLMPLWEIQEIMAGKP
                     GTKVELDFLRLGETVKASFELKEFEPPAPSLETLDRGVVMLRIPNFDGETAAQVRELL
                     KRPEAAAGGKAANGRLLVDLRGVSVGEASEAYATAGLFASGDLGALMRRAQEVEAFSG
                     QLAPSWKGKMVVLVDRGTLGPGEIFATVLRQKEIAELVGERTFGYAGRQEMAELSSGG
                     RLLITGAFYSGPDRKPLNESLKPDLLVNERSRTFLEREVPMTELILRRGVSRVLGEDQ
                     ETAKKAA"
     gene            complement(1655..2809)
                     /locus_tag="VEL74_18765"
     CDS             complement(1655..2809)
                     /locus_tag="VEL74_18765"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYG64629.1"
                     /translation="MARLARLRPAVCALFFLFLFLFLFLIPPVLAQEPSSGPSREQEL
                     TAIRAQIADLTSRLERARTQQSGLRGEMAAADLALELQEKRLAEAVAARDVAARRAAA
                     SEAEVSRLEAAVAKSRRDLGRSLAGLYRLGRQGYLRLFFTLRPDHRLLPSVRLMRFLA
                     RRDRQAVDRYQEARVRLAGQRERLVAEREEMERWLGQEQARRRQLIGLRQRKATLLAR
                     LEQEQRTLTARTADLADRERKLANFLDLLYGRNTAILAGTPMQQFRGILDWPVRGKVT
                     SGFGPRLDPRYRTQVPHNGVDLESAAGSEVKVVFPGKVLFAAPFQGYGQTVIVLHPGR
                     VFTLYAGLTDLRVGPEAMLSLGDVVGLASDKLYFEIRVENRPEDPLRWLR"
     gene            complement(2857..5079)
                     /locus_tag="VEL74_18770"
     CDS             complement(2857..5079)
                     /locus_tag="VEL74_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYG64630.1"
                     /translation="MYIRTTLLLAALTAAAAGAAESPMSAPAEKEPAASNAAAPAARF
                     VYPETRRVDHVDELHGEKIADPYRWLEDLDSKDTADWVAAQNKVTNAYLEKIPQRQAI
                     RQRLTELWNYERYGAPFKQGSRYFITRNDGLQNQSVLYTMETLDAKPRVLLDPNTLSA
                     DGTVALSGTAVSDDGRLIAYGLASAGSDWEEWRVRDVGTGKDLPDVVRWVKFSSAAWT
                     HDNKGFFYSRYDEPKEGQSLKDLNFNQKLYYHRLGTPQSADELVYERPDHKEWGFIPM
                     VSDDGRYLIVHVWKGTETENGVLYKDLTKPGSAPAPIVELLTGFDAAYDILGSHGSTF
                     WFATDLDAPMRRVIAIDVTRPQRENWREVIPQAKEALRGVQVFRDRFVANYLKDAHTQ
                     IRLFDLSGKPMGELPLPGLGSAGGFGGQWDDRETFYYFTGFTTPATIYRYDLDTGKST
                     VFRRPEVKFDPEAFETRQVFYQSKDGTRVPMFLTFKKGLKLDGTNPTYLYGYGGFNSS
                     MTPGFSVPQIVWMENGGIYAVANLRGGGEYGEEWHQAGSKLKKQNTFDDFIAAAEYLI
                     ANRYTSRQHLAIGGHSNGGLLVGAALNQRPDLFGAAIAGVGVMDMLRFHKFTIGWGWV
                     SDYGSPDNPEEFKVLRAYSPYHNIKEGIEYPAVLITTADHDDRVVPAHSFKYAAALQR
                     AQGGPEPVLIRIETRAGHGGGKPVSKQIEESTDEMGFLLNELTGGQAGGSAASPAAKR
                     "
     gene            complement(5199..7151)
                     /locus_tag="VEL74_18775"
     CDS             complement(5199..7151)
                     /locus_tag="VEL74_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64631.1"
                     /translation="MSDGTKKDGELEKVRSELRRLGYLDHGFERFLLQDALRPQRPLR
                     TLTQLTLKVGLLAGGALAFALALALAVANDTLSGGPWWAALLDLGALFLHLFPPVAAT
                     TALAFLALCGLILLAIRLYHVRRIETLSLAAAVAAGAAGLWLALWSARTVLPESGLPQ
                     LLLLGLVTPVAIYALVKLVYHGLLTLAIRFTDAPPRGRLFSGRWLGLAILSAAFILTL
                     PAVLSARRVELRTPATLPMAPGDRVLLLGIDGVLPEEVDYLLSLGDLPTLGRLAGPPG
                     APGAPGDNGGGRVWSYRRRVEPPASFWTSVATGRPGPDHGVSALDSFRPLGVRTPLER
                     SGPLRLWWSRVAVPLGIAEYRPVLANRRRAFTLWELASRGGAPVLAVNWWATFPAETL
                     PGQVVAHGAYQLLAEGVEGAVAPDTGLDRLAALAAEIGRAPWTGRPVTAALPPKAAAA
                     VLDRALRPDLFYREAFLRGLHASAPGASPSAMAPRAAALYLPGLDIAADGWSGGAVAF
                     ADLVREELVAADGVLARALAAGTAGAPGDEVGTIAVVFDPGRRREGGEGRILLWRRAG
                     CSGAAGGAGDAGARRPETSPEAVAAALLRAAGLPQSAELPAPPAGCPWPDPPVTFPGY
                     GEPRRTQTLPTEGGEYLESLRSLGYL"
     gene            complement(7151..7861)
                     /locus_tag="VEL74_18780"
     CDS             complement(7151..7861)
                     /locus_tag="VEL74_18780"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HYG64632.1"
                     /translation="MNGLLAPVQVGPVEMSFTGAAVCAILLLLSVWSTMVSADRLIVF
                     ARARRQSRAFARALAQDPGRPDWIRGALDAAARYPHSHLARVVAAGLAAFQKRSARGL
                     PHAEVIEAVNRAVDRSALRVTAEMRRGVSSLATIATTAPFIGLFGTVIGIIHAFDSIA
                     REGAGGFATVSAGISEALVATALGLVVAIPAAWMFNVLTHRLERLGAEMASSSSELMD
                     FFLDLATGEVDASATLGA"
     gene            complement(7864..8667)
                     /locus_tag="VEL74_18785"
     CDS             complement(7864..8667)
                     /locus_tag="VEL74_18785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HYG64633.1"
                     /translation="MFESSLIDLGDKTKRVQTRRCLSLPVAVGIHVVLAASVAFAAYW
                     NIAAVTEPSVNVVFFAEPPAPAVPSAPAPGPKPAAAAPKPVPVRPEQPVQPHDIPDEV
                     PVADPNPTPVPVVTFAGPVDDQSSDSSGGGGGSAMSSGGGGTDGGVCVGPECGDDPSS
                     LVPAGAPVTSSAPLAVGGAVTKPEAVYRVQPVYTEGARRIRLAGAVIVQAVIDEQGRV
                     TDVELVKGLPMGLDRAALDAVRQWRFKPATLHGRPVKVFFNLTVRFEVR"
     gene            complement(9072..10004)
                     /locus_tag="VEL74_18790"
     CDS             complement(9072..10004)
                     /locus_tag="VEL74_18790"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYG64634.1"
                     /translation="MPTDTATPAAAAAHATGQLAGQLAEWAGRVDRHLPSLLPAPSDR
                     PEAVHRAMHYALTSPGKRVRPVLTLAVAGMFGVRAEPVLDLACGVEMVHACSLVLDDL
                     PSMDDANLRRGRPTVHRAFGENVALLAALGLLNRAYALVAEGAHRLALRRYTAEDMIH
                     HLAAAIGSEGLIGGQALDLLSRPEELDLELLEYIHSHKTGALFMAAGELGAMAADAKR
                     RDLEVVSRYAKNLGLAFQISDDLLDVLSTPEETGKDTGKDTAKVTFVKLLGVAGAQAL
                     AEELLGFAVESLAPLGRKADPLRGLALFVRHRTR"
     gene            complement(10073..10414)
                     /locus_tag="VEL74_18795"
     CDS             complement(10073..10414)
                     /locus_tag="VEL74_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYG64635.1"
                     /translation="MKVNLRQREGVTILDLKGKITIGTGDIALRNAVQDAVNGGATQV
                     VINLADVSTIDSSGVGELVSAYTTATNRGARLKLTNLPPKITDVLTITQLITVFDVYD
                     TEDEAVRSFAA"
     gene            complement(10411..10893)
                     /locus_tag="VEL74_18800"
     CDS             complement(10411..10893)
                     /locus_tag="VEL74_18800"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG64636.1"
                     /translation="MPEPLHVHLAIGSRFENIELVQIVLKDSLVSLGLEEDALHWIDI
                     AVREAVANAIKHGNAQDPAKKVDVDLAIVGAELVIQVRDQGEGFDPGQIQNPLAPENL
                     LKPNGRGIFYMKSFMDEINYGLEPGGGTVVTMRKRLPGLAADSEIGKAPGTGDLEGKQ
                     "
     gene            complement(10959..12044)
                     /locus_tag="VEL74_18805"
     CDS             complement(10959..12044)
                     /locus_tag="VEL74_18805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HYG64637.1"
                     /translation="MSPIELLRVLGPLALAVVASFVLDRLCARKGLLPPGFREPFRRW
                     GAMFVVAVLLWIAVFAPLGNIGLGVEMDVTQVHPVQLFLLHALMLLVLGTWFLLGFGG
                     SAGAYRPLPSPPPSPLPSPVPAAVPVAGEPAGDPGVALEAASEAVIEPPPASPLPEGV
                     AAPLAEPVPAMAALPPPVSLGRRFAEQFGLVAPSVPREIGLGLTLGLGAWVAVLVAVM
                     LIGGLIVMLGGEDTLPQRPPQVVHWIAGLPLLLRCLVSLSAGVVEELFFRGFLQPRVG
                     IVLSTAFFVLAHFSYGQPFMLIGITILSLIYAFLVRWRQSVWAAIAAHVLFDAIQLLV
                     VIPAALRLLESQGRGAETSALLGLWPG"
     gene            complement(12051..12287)
                     /locus_tag="VEL74_18810"
     CDS             complement(12051..12287)
                     /locus_tag="VEL74_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64638.1"
                     /translation="MARDIAEGYAAVTERSFRNLTRADFEQLTFEIERYMRDLRGDQP
                     SLDDQQAIQQRNRRIQRLNTATVVMRSYRMKNKI"
     gene            12553..13797
                     /locus_tag="VEL74_18815"
     CDS             12553..13797
                     /locus_tag="VEL74_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYG64639.1"
                     /translation="MSRFPGVDLLDLDSLFNEEERLVRDTVRAFVDDRVKPIIEECHR
                     EGRTPLELVPEMGALNLFGASISEYGLPGLGGVAYGLIMQELERGDSGLRSFVSVQSA
                     LVMYPIYTFGSQEQKDRWIPALASGEAIGCFGLTEPDFGSNPGGMRTTARKVGDEWVL
                     NGSKQWITNGTLADVAVVWAKTEDGIRGFLVEKGTPGFTSSDQHGKFSLRASTTSELG
                     FHDCRIPAANMLPGTTGLKNALMCLNQARYGIAWGGIGSAMETYYTALEYAKQRVQFN
                     GQPIACHQLVQEKLVWMVSEISKGQLLCLHLGRLKDAGRLKHHHISMGKRNNVWVARE
                     CAKLAREVLGANGIVDDYPVIRHMLNIESVYTYEGTHDIHTLILGETVTGIPAYNAPE
                     RMEKAPKAKAEPAVAAVAGGGR"
     gene            13797..14780
                     /gene="glpX"
                     /locus_tag="VEL74_18820"
     CDS             13797..14780
                     /gene="glpX"
                     /locus_tag="VEL74_18820"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HYG64640.1"
                     /translation="MVMDHLLEQDFIRVTEQAAIAAAKTMGFGDRRHSDQVAVEAMRR
                     EMDSLAMDGRVVIGEGERDEAPMLYVGEEVGALRGKDGAVQVDIAVDPLEGTNLCALG
                     TPDAIAVLAAAERGGLLYAPDVYMEKIVVGPTARGSIHIDAPVAENLRNIAAAFNRKV
                     SDLTIVVLDRPRHEQLISDIREAGARIRLIPDGDLSAGISAAVRGTGIHAVMGTGGAP
                     EGVITAAAMRCLGGEIQARLKALDEKQQERVKALGFHDLSKIYRTEELAPGNEILFAS
                     SGVTDGELLRGVRFFGGGSRTSTLFMSLSRKIIRFVDTIHREELTTPVRFI"
     gene            14883..15371
                     /gene="dcd"
                     /locus_tag="VEL74_18825"
     CDS             14883..15371
                     /gene="dcd"
                     /locus_tag="VEL74_18825"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HYG64641.1"
                     /translation="MIKPDKWIREWGRRGGVTPFEEAQVNSASYDVRVSDHWICPTRD
                     PEEFRAPHIKLFPGEVVLASTMEYVRIPRDVACDLKLKSTLGRVWINHSLAGWCDPGF
                     AGQITLELQNLGPEPFILEAGRRIAQLIFISMESEPEIAYGEPGSSSHYQGQEGTTRA
                     RR"
     gene            15456..16610
                     /locus_tag="VEL74_18830"
     CDS             15456..16610
                     /locus_tag="VEL74_18830"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HYG64642.1"
                     /translation="MGIVGNLRTMQLEELLQWLSQSKKTGTLEFKGDKVEKKIFFKDG
                     RILASASNRPEEYLGHFLVSHGLISEQALSKAIELQEMTGMLLGKILVQKEAIGEKDL
                     HRMLRLKTEESIYDIFSWTEGEFHFVDDLIPESAMVPMALDVTGIVMEGVQRVDEWRR
                     IRAAVPNGDCIPVGVAQFDTRKLDAGEKQILELVDDSRSVEDIRLETHSSEFHVCRVL
                     FRQYQEGKLKIVKPRRGHNTGAHPVAVAAVAGPVTGQALLEAGQRYMEHGDWDLGLRH
                     LRAARSLEPDNPDMQDALAHGEKRIREEIERAGVSLASIPKMTASMEQLTSSKLSPQE
                     GFMLTRINGSYDIQSILKITPMAQLDALMVFWKLASQGYIKLEAPKAKRM"
     gene            complement(16617..16868)
                     /locus_tag="VEL74_18835"
     CDS             complement(16617..16868)
                     /locus_tag="VEL74_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017286717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HYG64643.1"
                     /translation="MRLPRDVSGDELARALASLGYRVIRQTGSHLRLTTEQGGQHHVT
                     IPRHDPLRLGTLAGILLDVAQHFGWTRQQVEERLFRLKP"
     gene            complement(16865..17071)
                     /locus_tag="VEL74_18840"
     CDS             complement(16865..17071)
                     /locus_tag="VEL74_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002747310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoisovalerate dehydrogenase"
                     /protein_id="HYG64644.1"
                     /translation="MNEVIFVVEEAPEGGYTARALGESIFTEGDDLAHLQEQVRDAVL
                     CHFDEDERPKMIRLHFVREELIAV"
     gene            17184..17567
                     /gene="crcB"
                     /locus_tag="VEL74_18845"
     CDS             17184..17567
                     /gene="crcB"
                     /locus_tag="VEL74_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HYG64645.1"
                     /translation="MARFLWICLGGAAGTGARYLLSLAMLRLAGPAFPWGTLLVNVLG
                     SFLLGLILEAQISTGWLSPTWNLALTTGVLGGFTTYSTFNYETLRLLQAGAWWLGIAN
                     LLGTVLGCLAAGALGFAFARWLVGS"
     gene            complement(18215..19585)
                     /locus_tag="VEL74_18850"
     CDS             complement(18215..19585)
                     /locus_tag="VEL74_18850"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HYG64646.1"
                     /translation="MFRNVLRAALLGSALLAAVLPASAQVDTGAANFTTYVAVGDSLT
                     AAFMSGGLVESAQRNSYPALIYRQATGRATGFEQPLVSAPGIPALQQLRSLLPLSIGQ
                     AAGTGTPLNATLQRPYNNLAVPGADVHDVLFTVRGGLNDLILRGLGTQLQQAVALQPT
                     FVTLWIGNNDALAAALAGVVDDNTLTPLATFTDEYRQIVNALNATGARMAIANIPNVT
                     VIPYVNTVPTVVVNPQTNQPVLNNGQPIPLIGPNGPLGAGDRVLLTGSAELAQGRGLP
                     PSLGGTGPLSDAAVLSASEIATINARVAAFNQVIQAEASRVGAAFVDMNAFLNQAATT
                     GLRVGGMTFTPAFLTGGIFSYDGVHPTPFGYAYIANVFIDEINEQFDAEIPPVDLYPF
                     LFGPKSGGGATFQAGQVVVKVDPIAVRNLFWALNVQQVTEDPGTGRNNRPADRPRNNR
                     RRNRKN"
     gene            complement(19619..20881)
                     /locus_tag="VEL74_18855"
     CDS             complement(19619..20881)
                     /locus_tag="VEL74_18855"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HYG64647.1"
                     /translation="MAALGVLAVVPVHAAGFSIFEQGTRAMGTAGAFTGQADDPSMLY
                     HNAGGLAFVEKSDWMVGATYITSSEAEFEGAGAFPGPGYRAEQETLAEILPHAYYVTP
                     INDRLKFGFGLYAPFGLTTSWENPDQFAGRFISTEAAIRSVDLNPTIGIQLTPNFGLG
                     IGAIARVSDVDLNRNVGTTNPFTFRFVDVGRLNLETDFGEGYGWNVGILHRWNNSFSW
                     GLSYRSNLTIDYEGDARLEQVSTGNAQLDQVIASRLPFNRDLPAEASIEFPDQASLGL
                     SFALSPNLRLNTDFNYMGWSSFDTTEIDFVNNDLPDSEIVSRWDDVYSYRAGLSWTAS
                     PSTQWRLGYVYDETPQPEEAVSPLLPDANRDGITLGWGHSGNWRIDVALMYLMFEERT
                     RARSFENEGDFFGTYNTTAWLFGLTVGR"
     gene            21126..21605
                     /locus_tag="VEL74_18860"
     CDS             21126..21605
                     /locus_tag="VEL74_18860"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HYG64648.1"
                     /translation="MPLEDRIQEETFEKLHEYLGELFEDPYHDPENDHFYVRYGTTVL
                     EISVEPYDSEEAVVIIMSYCVQDVELADDLLLGLLELNHQVPCGAFSLVDNDIFFSYS
                     LFGRHLSPRDLLRAITAVATVADDYDDRIVAKYGGQTALERIQDTGGRRRRQGYGEE"
     gene            complement(21628..>21867)
                     /locus_tag="VEL74_18865"
     CDS             complement(21628..>21867)
                     /locus_tag="VEL74_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HYG64649.1"
                     /translation="PPERDRKLLLQRRQIERLFGKTQLKGLTVVPLSVYLKGNLIKLE
                     IALVQGKKQFDKRQATKERELDREMEAAVKSGGRY"
BASE COUNT         3356 a         7491 c         7843 g         3177 t
ORIGIN      
        1 acccggccca gcttgccgcc ctctccggcc ggcgcggcct cgtcgcctcc ctcgtccccc
       61 ggctccgtgc tcgcctccgg cggcggctga tcgccttccg gagcgtccgg cccctccgaa
      121 gtggcggctt cgctggctcc actggctccg gtggcccgtg gctccagccg cccctcgcgc
      181 gcggcctccg tggcctcggc ctcctgggta gccgtggcca cctcccggcc ggagtccccg
      241 ggggtgtcgc ggaacgccat gtagaggccg ccggcgccga ccagcaggcc gagcaggaag
      301 agccagaggc ccgagacgcc ggaacggcgg gcacggccgg agcggcgggg gacaggtcgg
      361 ctggaacggc gatacgccat cagacttcta tgcggtgtgg agatgagggc gcggccccgg
      421 ggaggacccg gggccgcgga ccggtcaggc agccttcttc gccgtctcct ggtcctcgcc
      481 gaggacgcgg ctgacgccgc gccgcaggat cagctcggtc atcggcacct cgcgctccag
      541 gaaggtgcgc gagcgctcgt tgaccagcag gtcgggctta aggctctcgt tcagcggctt
      601 gcggtcgggg ccggagtaga aggcgccggt gatcagcagc cggccgccgc tcgacagctc
      661 cgccatctcc tggcgtccgg catagccgaa ggtccgctcg cccaccagct cggcgatctc
      721 cttctggcgc agcacggtag cgaagatctc gccggggccg agggtgccgc ggtccaccag
      781 caccaccatc ttgcccttcc aggacggcgc gagctggccg gagaaggcct cgacctcctg
      841 ggcccgccgc atcagcgcgc cgaggtcgcc gctggcgaac aggccggcgg tggcgtacgc
      901 ctcgctggct tcgccgaccg agacgccgcg caggtcgacg agcagccggc cattggccgc
      961 cttcccaccg gcggccgcct cgggccgctt gagcagctcc cgcacctggg cggcggtctc
     1021 gccgtcgaag ttggggatgc gcagcatgac gacgccgcgg tcgagggtct ccaggctggg
     1081 cgccggcggc tcgaactcct tgagctcgaa ggaggccttg accgtctcgc cgaggcggag
     1141 gaagtcgagc tccaccttgg tgcccggctt gccggccatg atctcctgga tctcccagag
     1201 cggcatcagg cgggtcgacc ggccgtccac cttggagatg aggtcgccgg gctgcacgcc
     1261 ggcgaggccg gcggggctcc ccttctccac cgtcaccacg taggcgatgc cggactcctt
     1321 gagcagggtc atgccggtgt ggcggcggcc gacggcgcgg gcgtcgaggt agccctcgac
     1381 ctggccggcc gggatgtaga gggagaaggg gtccagcgcg tcggtggtgc cgtccagcgc
     1441 gccggccatc agcgcgttca tgtcgggctc gtcgacgtgg gcctggcgga tcatgccgag
     1501 cacctcgctg aagatcgcga ggtacttgta cagggagtct tcctgcggct cctggcggtc
     1561 ggccgccaga agcagcgtgc cggcgaggat cgggaagacg aggacggcgg agacgagaaa
     1621 gaatatgacg cgactgcggc tcatgtttac gtacctaccg taaccagcgc aaagggtcct
     1681 cgggacgatt ctccacgcgg atctcgaagt agagcttgtc cgatgccagc cccacgacgt
     1741 cccccaagga taacatggct tccgggccca cccgcaggtc ggtgagcccc gcgtacagcg
     1801 tgaagacgcg gcccgggtgg aggacgatca cggtctggcc gtacccctgg aagggggcgg
     1861 cgaagagcac tttgccgggg aagacgactt tgacctcgga gcccgcggcg ctctcgaggt
     1921 cgactccgtt gtggggcacc tgggtgcggt agcgcgggtc gagacggggg ccgaagcccg
     1981 acgtcacctt gccgcggacc ggccagtcga ggatgccccg gaactgctgc atgggggtgc
     2041 cggcgaggat ggcggtgttg cggccgtaga gcaggtcgag gaagttggcc agcttgcgct
     2101 cgcggtcggc gaggtcggcc gtccgcgcgg tgagggtgcg ctgctcctgc tccaggcggg
     2161 cgagcagggt cgccttgcgc tggcgcaggc cgatgagctg gcggcggcgc gcctgctcct
     2221 ggccgagcca gcgctccatc tcctcgcgct cggcgaccag ccgctcgcgc tgcccggcca
     2281 ggcggacccg ggcctcctgg tagcggtcca cggcctggcg gtcgcgccgg gcgaggaagc
     2341 gcatcaggcg gaccgacggc aggagccggt ggtcgggccg cagggtgaag aagagacgca
     2401 gatagccctg gcggccgagc cggtagaggc cggcgaggct ccgccccagg tcgcgccggg
     2461 acttggccac cgccgcctcc aggcggctca cctcggcttc ggaggcggcg gcgcgccgcg
     2521 ccgccacgtc gcgggcggcg accgcctcgg cgagccgctt ctcctgcagc tccagggcga
     2581 ggtccgccgc cgccatctcg ccgcgcagcc cgctctgctg cgtccgcgcc cgttcgagcc
     2641 gcgacgtcag gtcggcgatc tgcgcccgga tggccgtcag ctcctgctcc cgggacgggc
     2701 ccgacgacgg ttcctgcgcc aggacagggg ggatgaggaa gaggaagagg aagaggaaga
     2761 ggaagaagag ggcgcagacg gcgggccgga gtctggcaag tcttgccatc gtctctcccg
     2821 gccgcctgcg cgtcattccg tggaagcgac cgtccgctac cgcttcgcgg ccgggctggc
     2881 ggcgctcccg ccagcctgcc cgccggtcag ctcgttgagg aggaagccca tctcgtcggt
     2941 ggactcctcg atctgcttgc tgaccggctt gccgccgccg tggccggcgc gggtctcgat
     3001 gcggatcagc accggctcgg ggcccccctg ggcccgctgc agcgccgcgg cgtacttgaa
     3061 gctgtgcgcc ggcaccacgc ggtcgtcatg gtccgcggtg gtgatcagca ccgccggata
     3121 ctcgatgcct tccttgatgt tgtggtacgg cgagtaggcg cgcaggacct tgaactcctc
     3181 cgggttgtcc ggcgagccgt agtccgacac ccacccccag ccgatggtga acttgtggaa
     3241 gcgcagcatg tccatgacgc cgacgccggc gatggcggcg ccgaacaggt cggggcgctg
     3301 gttgagcgcc gcgcccacca gcaggccgcc gttgctgtgg ccgccgatcg ccaggtgctg
     3361 gcgcgacgtg tagcggttgg cgatcaggta ctccgccgcc gcgatgaagt cgtcgaaggt
     3421 gttctgcttc ttgagcttgc tgccggcctg gtgccactcc tcgccgtact cgccgccgcc
     3481 gcggaggttg gccaccgcgt agatgccccc attctccatc cagacgatct gcggcacgct
     3541 gaaccccggg gtcatcgacg agttgaagcc gccgtacccg tagaggtagg tggggttggt
     3601 gccgtccagc ttgaggccct tcttgaaggt gaggaacatc ggcacccgcg tgccgtcctt
     3661 gctctggtag aagacctggc gggtctcgaa ggcctcgggg tcgaacttga cctccgggcg
     3721 ccggaagacg gtgctcttgc cggtgtcgag gtcgtaccgg tagatggtgg ccggcgtggt
     3781 gaagccggtg aagtaataga aggtctcgcg gtcgtcccac tggccgccga agccgccggc
     3841 cgagccgagg ccgggcaggg gcagctcgcc catcggcttg cccgacaggt cgaacaggcg
     3901 gatctgggtg tgggcgtcct tcagatagtt ggcgacgaac cggtcgcgga acacctggac
     3961 gccgcgcagg gcctccttgg cctgcgggat cacctcccgc cagttttcgc gctgcggcct
     4021 ggtgacgtcg atggcgatca cccggcgcat cggggcgtcg aggtcggtgg cgaaccagaa
     4081 ggtggagccg tggctgccca ggatgtcgta ggcggcgtcg aagccggtga gcagctccac
     4141 gatcggagcg ggcgccgagc cgggcttggt gaggtccttg tagaggacgc cgttctcggt
     4201 ctccgtcccc ttccagacgt ggacgatgag atagcggccg tcatcgctca ccatcgggat
     4261 gaagccccac tccttgtggt ccggccgctc gtagaccagc tcgtccgcgg actgcggcgt
     4321 ccccagccgg tggtagtaga gcttctggtt gaagttcagg tccttgagcg actgcccctc
     4381 cttgggctcg tcgtagcgcg agtagaagaa gcccttgttg tcgtgggtcc aggcggcgct
     4441 cgagaacttc acccagcgca cgacgtccgg caggtccttg ccggtgccga cgtcgcgcac
     4501 ccgccactcc tcccagtcgg agccggcgga ggccaggccg taggcgatga gcctgccgtc
     4561 gtcgctcacg gcggtgcccg acagggccac ggtgccgtcg gccgacaggg tgttggggtc
     4621 gaggagcacc cgcggcttcg cgtccagcgt ctccatggtg tagaggacgc tctggttctg
     4681 caggccgtcg ttgcgggtga tgaagtagcg gctcccctgc ttgaacgggg cgccgtagcg
     4741 ctcgtagttc cacagctcgg tcagccgctg gcggatcgcc tggcgctgcg ggatcttctc
     4801 cagataggcg ttcgtcacct tgttctgggc ggccacccag tcggcggtgt ccttggagtc
     4861 caggtcctcg agccagcggt aggggtcggc gatcttctcg ccgtgcagct cgtccacgtg
     4921 gtcgacccgg cgggtctcgg ggtagacgaa gcgcgccgcc ggggcggcgg cgttcgaagc
     4981 ggcgggctcc ttctcggccg gcgcgctcat gggcgactca gcggctcccg cggcggccgc
     5041 cgtcagcgcg gccagcagca gagtggtgcg gatgtacatg aagtcctcct gtcgaagcca
     5101 cggtcgaagc ggctttcgaa gcgattgggg gccgggcgag ggccgttttt acaaccgacg
     5161 atccgggggc ggacattcca tgtctacggg agagagggtc aaagatatcc cagcgagcgg
     5221 aggctttcca ggtactcccc cccttcggtg gggagcgtct gggtgcgccg gggctcgccg
     5281 tagccgggga aggtgaccgg cgggtcgggc caggggcagc ccgccggcgg cgcgggcagc
     5341 tccgcgctct gcggcagccc ggcggcgcgc agcagggcgg cggccacggc ctcgggggag
     5401 gtctcgggcc gccgggcgcc ggcatctccc gcaccgccgg ccgcgccgga gcacccggca
     5461 cggcgccaga ggaggatccg cccctcgccc ccttcgcggc ggcgccccgg atcgaagacc
     5521 acggcgatcg tccccacctc gtcccccggc gccccggcgg tcccggcggc caacgcccgg
     5581 gctagcacgc cgtccgcggc caccagctcc tcccgcacca ggtcggcgaa ggcgacggcg
     5641 ccgccgctcc agccgtcggc ggcgatgtcg aggcccggga ggtagagggc ggccgcgcgc
     5701 ggcgccatcg ccgaggggga agcgccgggg gcggaggcgt gcagcccccg gaggaaggct
     5761 tcgcgataga agaggtcggg gcgcagcgcc cggtcgagca ccgccgccgc ggccttcggg
     5821 ggcagcgcgg cggtgaccgg cctccccgtc cacggcgccc gtccgatctc cgcggcgagg
     5881 gcggcgaggc ggtccaggcc ggtatcggga gccaccgcgc cctccacccc ttcggccagg
     5941 agctgatacg ccccgtgggc caccacctgt cccggcagcg tctcggccgg gaaggtcgcc
     6001 caccagttga ccgccaggac cggcgcgccg ccgcgggagg ccagctccca gagggtgaag
     6061 gcgcgccgcc ggttggcgag caccggccgg tactcggcga tccccagggg gaccgccacc
     6121 cggctccacc agaggcgcaa cggcccgctc cgctcgagcg gcgtcctcac ccccagcggc
     6181 cggaagctgt cgagcgccga gacgccgtgg tccgggcccg gccggccggt ggccaccgag
     6241 gtccagaagg aggccggcgg ctccacccgg cgccggtagc tccacacccg tccgcccccg
     6301 ttgtctcccg gcgctcccgg cgctcccggc ggcccggcga gccgcccgag ggtcggcagg
     6361 tcgccgagcg acagcaggta gtccacctcc tcggggagga cgccgtcgat ccccagcagc
     6421 agcacccggt cgcccggcgc catggggagc gtcgccgggg tccggagctc cacccgccgc
     6481 gccgacagca ccgccggcag ggtgaggatg aatgccgccg agaggatggc gaggcccagc
     6541 cagcggcccg agaacagccg cccgcggggg ggcgcgtcgg tgaagcggat cgccagcgtc
     6601 agcaggccgt ggtagaccag cttgaccagc gcgtagatcg ccaccggcgt caccaggccg
     6661 aggagcagga gctgcggcag cccgctctcc ggcagcacgg tgcgggcgct ccacagggcg
     6721 agccacagcc cggcggcgcc ggcggccacg gcggcggcga gcgacagcgt ctcgatgcgc
     6781 cgcacgtggt agaggcggat ggcgaggagg atcaggccgc agagcgcgag gaacgccagg
     6841 gcggtcgtgg ccgccaccgg cgggaagagg tgcaggaaga gggcgcccag gtcgagcagc
     6901 gcggcccacc agggcccgcc gctcagggtg tcgttggcga ccgccagggc gagggcgagg
     6961 gcgaaggcca gcgcgccgcc ggcgaggagc ccgaccttga gggtgagctg cgtcagggtg
     7021 cgcagcggcc gctgcgggcg cagggcgtcc tggagcagga agcgctcgaa gccgtggtcg
     7081 agatacccca gccgccgcag ctccgagcgg accttctcca gctcgccgtc cttcttcgtc
     7141 ccatcgctca tcaggcccct agtgtagcgg acgcgtccac ctcccccgtc gcgaggtcga
     7201 ggaagaagtc catcagctcc gaggaggagc tcgccatctc ggcccccagg cgctcgaggc
     7261 ggtgggtgag gacgttgaac atccaggcgg cggggatcgc caccaccagg ccgagggcgg
     7321 tggcgaccag ggcctcggag atccccgccg agaccgtggc gaagccgccg gccccctccc
     7381 gggcgatgct gtcgaaggcg tggatgatgc cgatcaccgt gccgaacagg ccgatgaacg
     7441 gcgccgtggt ggcgatggtg gcgaggctgg agacgccgcg ccgcatctcg gccgtgacgc
     7501 gcaacgccga gcggtccacg gcccggttca cggcctcgat gacctcggca tggggcagcc
     7561 cccgggcgct ccgcttctgg aaggcggcga gcccggcggc caccacccgc gccaggtggc
     7621 tgtgcggata gcgcgccgcg gcgtcgaggg cgccgcggat ccagtccggg cgcccggggt
     7681 cctgggcgag ggcccgggcg aaggcgcggg actgccgccg cgcccgggcg aagacgatca
     7741 gccggtcggc cgagaccatc gtggaccaga ccgacagcag gaggaggatg gcgcacacgg
     7801 cggcgcccgt gaagctcatc tccaccgggc cgacctgcac gggagcgaga aggccgttca
     7861 tgctcagcgc acctcgaaac gcaccgtgag gttgaagaag accttgaccg gccgtccgtg
     7921 cagcgtggcc ggcttgaacc gccactgccg gaccgcgtcc agggccgccc ggtcgagccc
     7981 catggggagc cccttcacca gctcgacgtc ggtcacgcgc ccctgctcgt cgatgaccgc
     8041 ctggacgatc accgcgccgg ccaggcggat cctgcgcgcc ccctcggtgt agacgggctg
     8101 cacccggtac acggcctccg gcttggtgac ggcgccgccc accgccagcg gggcgctgct
     8161 cgtcaccgga gcccccgccg ggaccaggct cgacggatcg tcgccgcact cgggcccgac
     8221 gcagacgccg ccgtcggtgc cgcccccgcc cgacgacatt gcggacccgc cgccgccgcc
     8281 cgacgaatcg ctgctctggt cgtccacggg tccggcgaaa gtgaccaccg gcacgggggt
     8341 ggggttggga tcggccaccg gcacctcgtc ggggatgtcg tgcggctgca ccggctgctc
     8401 gggcctgacc ggcaccggct tgggagcggc ggcggcgggc ttcggacccg gcgccggcgc
     8461 ggaggggacc gccggcgccg gcggctcggc gaagaagacg acgttcaccg acggctccgt
     8521 gaccgcggcg atgttccagt aggcggcgaa ggcgacggag gcggccagca ccacgtggat
     8581 gcccacggcc acgggcagcg acagacaacg gcgggtctgc acccgcttcg tcttgtctcc
     8641 cagatcgatc aacgagctct cgaacatttc gcacctccgg cgcgactgag attctgatcg
     8701 gctccccgcc gagctccttg ccggcggagg gtttatccgg gaggcctccc ttgtctgtat
     8761 tagaggggag gagggggatt ttcgttccag gggactctgt gagccccgcg ccgcgatctc
     8821 catacccacg gataggaaga cgatacccag ggcgggaagg cgataccccg gggtatgacc
     8881 tccatacccc aggcaggaag gccctacccg aagagatgac ctccctaccc cgggtacgta
     8941 gcccatcccc ccaggagacg aaggacatcc cccgagagat gaccgccgta ccccgggtag
     9001 gacgaacgtt tctccgggga tggcggtccc cctcgcgggg caacggctgc cctgggaggg
     9061 acgacgaggt actagcgcgt ccggtggcgg acgaagaggg ccaggccgcg cagcgggtcg
     9121 gccttgcggc ccaggggggc gagggactcg acggcgaagc cgaggagctc ctcggccagc
     9181 gcctgggcgc cggcgacgcc gaggagcttc acgaaggtca ccttggcggt gtccttgccg
     9241 gtgtccttgc cggtctcctc cggcgtcgac aggacgtcga gcaggtcgtc ggagatctgg
     9301 aaggcgaggc cgaggttctt ggcgtagcgg ctgaccacct cgaggtcgcg ccgcttggca
     9361 tcggcggcca tggcgcccag ctcgccggcg gccatgaaca gggcgccggt cttgtggctg
     9421 tggatgtact ccagcagctc caggtccagc tcctcgggcc tggagaggag gtcgagcgcc
     9481 tggccgccga tcagcccctc gctgccgatg gccgccgcca ggtggtggat catgtcctcg
     9541 gcggtgtagc ggcgcagcgc cagccggtgc gccccctcgg ccaccagggc gtaggcgcgg
     9601 ttgagcaggc cgagcgcggc gagcagggcg acgttctcgc cgaaggcgcg gtgcaccgtg
     9661 ggccggccgc gccgcaggtt ggcgtcatcc atggacggca ggtcgtcgag caccagcgag
     9721 caggcgtgga ccatctcgac gccgcaggcg aggtccagga ccggctcggc gcggacgccg
     9781 aacatcccgg ccacggcgag agtgagcacc ggccgcaccc gcttgcccgg gctggtcagg
     9841 gcgtagtgca tggcgcggtg gaccgcctcc gggcggtcgg acggcgccgg cagcagggac
     9901 ggcaggtggc ggtccacccg gccggcccat tcggcgagct ggccggcgag ctgacccgtg
     9961 gcatgggccg ccgcggcggc tggagtggcg gtgtcggtcg gcatgggact gctacgggcc
    10021 cggccccggc cgggaggccg gcggggccgg gagagacgga cttcgaagcc gctcaggcgg
    10081 cgaaggagcg gacggcctcg tcctcggtgt cgtagacgtc gaagacggtg atgagctggg
    10141 tgatggtcag gacgtcggtg atcttgggag ggaggttggt gagcttgagc cgggcccccc
    10201 ggttggtggc cgtggtgtag gcgctgacca gctcgccgac gcccgacgag tcgatggtcg
    10261 acacgtcggc caggttgatc acgacctggg tcgcgccgcc gttgaccgcg tcctgcacgg
    10321 cgttgcgcag ggcgatgtct cccgtgccaa tggtgatctt ccccttgagg tccagaatcg
    10381 tcacgccctc acgctgccgc aggttcactt tcattgcttt ccctccaaat ccccggtccc
    10441 cggggccttg ccgatttctg aatcggccgc gaggccgggg aggcgtttgc gcatggtcac
    10501 gaccgtgcct ccccccggct cgaggccgta gttgatctcg tccatgaagc tcttcatata
    10561 gaagatgccg cggccgttgg gcttgagcag gttctccggc gccagggggt tctggatctg
    10621 cccggggtcg aagccctccc cctggtcgcg cacctggatc accagctccg cccccacgat
    10681 ggccaggtcg acgtcgacct tcttggcggg gtcctgggcg ttgccgtgct tgatggcgtt
    10741 ggccaccgcc tcgcggaccg cgatgtcgat ccagtgcaag gcgtcctcct ccagtccgag
    10801 cgagacgagg gagtccttga ggacgatctg caccagctcg atgttctcga agcggctgcc
    10861 gatcgcgaga tggacgtgga gcggttcagg catcgacact ggtcccggaa gcgagtctat
    10921 cagcagatcc cccccgccgc gccagccggg ggccgacgct atccgggcca cagccccagc
    10981 agggccgagg tctccgcgcc ccgcccctgc gactccagca ggcgcagggc cgccgggatc
    11041 accaccagga gctggatggc gtcgaacagg acgtgcgcgg cgatggccgc ccagacgctc
    11101 tgccgccagc gcaccaggaa agcgtagatc agcgacagga tggtgatgcc gatcagcatg
    11161 aagggctggc cgtaggagaa gtgggccagg acgaagaagg cggtcgacag cacgatcccg
    11221 acgcgcggct ggaggaagcc gcggaagaag agctcctcca ccacccccgc cgacaggctc
    11281 accaggcagc gcaggagcag gggcaggccg gcgatccagt ggacgacctg cggcggccgc
    11341 tgggggagcg tgtcctcccc gcccagcatc acgatcaggc cgccgatcag catcaccgcc
    11401 accagcaccg cgacccaggc gcccaggccg agcgtcagcc cgaggccgat ctcccgcggc
    11461 acgctgggcg ccacgagccc gaactgctcg gcgaagcgcc ggcccaggga gaccggagga
    11521 ggcagggcgg ccatcgccgg gaccggctcc gcgagcggcg ccgccacccc ctccggcaga
    11581 ggcgaggccg gcggcggctc gatgaccgcc tcggaggcgg cctccaaggc gacgccggga
    11641 tctccggccg gctctccggc cacggggacg gcggccggta ctggcgacgg caggggcgac
    11701 ggcgggggcg acggcagggg ccggtaggcg ccggcgctgc cgccgaagcc cagcaggaac
    11761 caggtgccga gcaccaggag catcagcgcg tgcagcagga agagctgcac cggatgcacc
    11821 tgggtgacgt ccatctcgac ccccaggccg atgttgccca gcggcgcgaa gaccgcgatc
    11881 cagagcagga cggcgacgac gaacatcgcg ccccaccggc ggaagggctc gcggaagccg
    11941 ggggggagca gccccttgcg ggcgcacagg cggtccagga cgaaggaggc gacgacggcc
    12001 agggccaggg gtcccaggac ccggaggagc tcgatcggcg acatggcagg ctagatcttg
    12061 ttcttcatgc ggtagctgcg catgaccacg gtggcggtgt tgaggcgctg gatgcgccgg
    12121 ttgcgctgct ggatggcctg ctggtcgtcc agagacggct ggtccccccg cagatcgcgc
    12181 atgtagcgct cgatctcgaa agtgagctgc tcgaaatcgg cacgggtgag gttgcggaag
    12241 ctgcgctcgg tgacggcggc gtagccctcc gcgatgtccc gcgccatcgc ccatgcgctt
    12301 cccccgggaa atcccatcgg gcgggatcat agccgatccg gcagcgcgtc cctatatcta
    12361 taaggatggg gctgcccgcc cggcgcaggc gaccggcagc ggccgggggg aggggggagc
    12421 gcacacgagg gagccgagca gggcgggcag ccccatccgt ggcgcctaga ggggccgtgg
    12481 tatcattccg ccccgcggtc tagagaccgg cccgcccgca tctcgcgagc cccaagcgaa
    12541 ggaggattcc ccttgagccg cttccccggc gtggacttac tggatttgga ctcgctcttc
    12601 aacgaagagg agcggttggt gcgcgacacg gttcgcgctt ttgtcgatga cagggtcaag
    12661 cccatcatcg aggagtgcca tcgcgagggg cggacgccgc tcgagctggt gcccgagatg
    12721 ggagccctca acctcttcgg cgccagcatc tcggagtacg gcctccccgg cctcggcggc
    12781 gtcgcctacg gcctgatcat gcaggagctg gagcgcggcg actcggggtt gcgcagcttc
    12841 gtctccgtgc agagcgccct ggtcatgtac ccgatctaca ccttcggctc ccaggagcag
    12901 aaggaccgct ggatcccggc cctggcctcc ggcgaggcga tcggctgctt cggcctgacc
    12961 gagcccgatt tcggctccaa ccccggcggc atgcgcacca ccgccaggaa ggtgggcgac
    13021 gagtgggtgc tgaacggctc caagcagtgg atcaccaacg gcacgctcgc cgacgtggcg
    13081 gtggtctggg ccaagaccga ggacggcatc cgcggcttcc tggtcgagaa ggggacgccc
    13141 ggcttcacct cctcggacca gcacggcaag ttctccctgc gcgcctccac cacctcggag
    13201 ctcggcttcc acgactgccg catcccggcc gccaacatgc tccccggcac cacggggctc
    13261 aagaacgccc tgatgtgcct caaccaggcg cgctacggca tcgcctgggg cggcatcggc
    13321 tcggccatgg agacctatta cacggcgctc gagtacgcca agcagcgcgt ccagttcaac
    13381 ggccagccga tcgcctgcca ccagctcgtg caggagaagc tggtctggat ggtctccgag
    13441 atcagcaagg ggcagctcct ctgcctgcac ctcggacgcc tcaaggacgc cggccgcctc
    13501 aagcaccacc acatctcgat gggcaagcgc aacaacgtct gggtggcccg cgagtgcgcc
    13561 aagctcgccc gcgaggtcct gggcgccaac ggcatcgtcg acgactaccc ggtgatccgc
    13621 cacatgctga acatcgagtc ggtctacacc tacgagggga cgcacgacat ccacacgctg
    13681 atcctcggcg agaccgtcac cggcatcccc gcctacaacg cgccggagcg gatggagaag
    13741 gctccgaagg ccaaggccga gcccgccgtc gcggcggttg ccgggggagg gcgttgatgg
    13801 tcatggacca cctcctcgag caggacttca tccgcgtcac cgagcaggcg gccatcgccg
    13861 ccgccaagac catgggcttc ggcgaccggc ggcactccga ccaggtggcc gtcgaggcca
    13921 tgcgccggga gatggacagc ctggcgatgg acggccgcgt cgtcatcggc gagggcgagc
    13981 gcgacgaggc ccccatgctc tacgtcggcg aagaggtcgg cgccctgcgc ggcaaggacg
    14041 gcgccgtgca ggtggacatc gccgtcgatc cgctggaggg gaccaacctc tgcgccctcg
    14101 gcacccccga cgccatcgcc gtgctggccg cggcggagcg cggcggtctc ctctacgcgc
    14161 cggacgtcta catggagaag atcgtggtcg gccccaccgc ccgcggctcc atccacatcg
    14221 acgccccggt ggcggagaac ctgcgcaaca tcgccgccgc cttcaaccgc aaggtgagcg
    14281 acctgaccat cgtggtcctc gaccggccgc gccacgagca gctcatcagc gacatccggg
    14341 aagcgggcgc gcgcatccgc ctgatcccgg acggcgacct gtcggcgggc atctcggcgg
    14401 cggtgcgcgg caccggcatc cacgccgtga tggggaccgg cggcgcgccc gagggggtca
    14461 tcaccgcggc ggccatgcgc tgcctgggcg gcgagatcca ggcgcgcctc aaggcgctcg
    14521 acgagaagca gcaggagcgc gtcaaggcgc tgggcttcca cgacctgagc aagatctacc
    14581 gcaccgagga gctcgctccc ggcaacgaga tcctgttcgc cagctccggc gtcaccgacg
    14641 gcgagctgct gcgcggcgtc cgcttcttcg gcggcggctc gcgcacctcg accctcttca
    14701 tgtcgctgtc gcgcaagatc atccggttcg tcgacaccat ccaccgcgaa gagctgacga
    14761 caccggtccg cttcatctag aggtcgtttt ccttcatcgg gaccatatgc gggccgcgtc
    14821 aacgccagca gggcggagcg cggcccgcgc ggtctccggc accctcccag gaggactgga
    14881 gcatgatcaa gccggacaag tggatccgcg agtggggccg gcgcggcggc gtcaccccgt
    14941 tcgaagaggc ccaggtcaac tccgccagct acgacgtgcg ggtgagcgac cactggatct
    15001 gccccacccg cgaccccgaa gagttccggg cgccccacat caagctcttc ccgggcgagg
    15061 tggtcctcgc ctcgaccatg gagtacgtcc gcatcccgcg cgacgtcgcc tgcgacctga
    15121 agctcaagtc gaccctgggg cgggtgtgga tcaaccactc gctggccggc tggtgcgacc
    15181 ccggctttgc gggccagatc accctggagc tgcagaacct gggtccggag ccgttcatcc
    15241 tcgaggcagg caggcgaatt gcacagctca tcttcatctc gatggagtcc gaacccgaga
    15301 tcgcctatgg cgagccgggc tccagcagcc actaccaggg ccaggaggga accacccggg
    15361 cccgaagatg aaggtgagga cccccgatgt acgcggtgcc cagctcggaa atccgttcaa
    15421 ggtattcgcc tagaactata atcggaccgg tcatcatggg catcgtcggg aatctgcgca
    15481 ccatgcagct cgaagagctg ctgcagtggc tctctcagag caagaagacc ggcacgctcg
    15541 aattcaaggg cgacaaggtc gaaaagaaga tctttttcaa ggatgggcgc atcctggcct
    15601 cggcctcgaa ccgtccggaa gagtacctgg gacacttcct ggtgagccat gggctgatca
    15661 gcgagcaggc cctctccaag gccatcgagc tgcaggagat gaccggcatg ctgctgggca
    15721 agatcctggt gcagaaggag gccatcggcg agaaggacct gcaccggatg ctccgcctca
    15781 agaccgagga gagcatctac gacatcttct cgtggaccga gggggagttc cacttcgtcg
    15841 acgacctgat tcccgagagc gccatggtgc cgatggcgct ggacgtcacc ggcatcgtga
    15901 tggagggggt gcagcgggtc gacgaatggc gccgcatccg ggcggcggtg ccgaacggcg
    15961 actgcatccc ggtcggggtg gcgcagttcg acacccgcaa gctcgatgcg ggcgagaagc
    16021 agatcctgga gctggtcgac gactcccgtt cggtggagga catccggctg gagacccact
    16081 ccagcgagtt ccacgtctgc cgcgtgctct tccggcagta ccaggaggga aagctcaaga
    16141 tcgtcaagcc gcgccgcggc cacaacaccg gcgcgcaccc ggtggcggtc gccgccgtcg
    16201 ccggcccggt caccgggcag gcgctgctgg aggcgggtca acggtacatg gagcacgggg
    16261 actgggacct cggcctccgc cacctgcggg ccgcccgcag cctggagccg gacaacccgg
    16321 acatgcagga cgccctcgcc cacggcgaga agcgcatccg ggaggagatc gagcgcgccg
    16381 gcgtctccct cgcctcgatc ccgaagatga cggcgagcat ggagcagctc acctcgtcga
    16441 agctctcccc gcaggagggc ttcatgctga cccgcatcaa cggcagctac gacatccagt
    16501 cgatcctgaa gatcaccccg atggcccagc tcgacgccct gatggtcttc tggaagctcg
    16561 ccagccaggg ttacatcaag ctcgaggcgc cgaaggcgaa gcggatgtag ggggcttcac
    16621 ggcttcagcc ggaacaggcg ctcctcgacc tgctgccgcg tccacccgaa gtgctgagcg
    16681 acatcgagca gaatcccggc cagcgtcccg agcctcagag gatcgtgacg ggggatggtg
    16741 acgtggtgct gcccgccctg ctccgtggta agccgcaggt ggctgcctgt ctgccggatg
    16801 acgcggtatc ccaaagaggc gagcgcccgg gcaagctcgt cgcccgagac atcccgaggg
    16861 agcctcatac cgcgatcagt tcctcgcgca cgaagtggag ccggatcatc ttgggacgtt
    16921 cgtcttcatc gaagtggcag agcaccgcat cccgaacctg ctcctggagg tgggccaagt
    16981 cgtccccttc ggtgaagatg gattcaccca aggcacgcgc cgtgtacccc ccttcgggcg
    17041 cctcctcgac gacaaagatc acctcgttca tggcagaccg actcctggta caggcccccc
    17101 ggacatgagg ggtgggacga gtctaccgcg actccgctcc cgtggcgacc ggagggctcc
    17161 ggcccaatgg tagagtccgt ccgatggccc gcttcctctg gatctgcctg ggaggcgccg
    17221 ccgggacggg cgcgcgctat ctgctgtcgc tggcgatgct gcgcctggcc ggaccggcgt
    17281 tcccctgggg gacgctattg gtcaacgtcc tcgggtcgtt cctgctgggc ttgatcctgg
    17341 aggcccaaat ctccaccggc tggctgtctc ccacctggaa cctcgccctc accaccggcg
    17401 tgctgggcgg cttcaccacc tactccacct tcaactatga gaccctgcgc ctccttcaag
    17461 caggggcttg gtggctcggc atcgccaacc tcctgggcac cgtcctcggc tgcctggccg
    17521 ccggagcgct cggcttcgcg ttcgcgcgct ggctggtggg aagctagacc tacgcgccac
    17581 aaggggcggt ggtgcctccc cccctgggag gggggccggt tagggagtgg gggaggttag
    17641 gaggggggtt ttgcggtgcc gcgcgccacc tccacggccc ccagcgaaat aaggtccatt
    17701 cctccggggc aaccgttgcc ccaggagaaa tggcggtcat tcctcatggg gcagtcattg
    17761 ccccttcaga aacggcctcc attcctggtg gggcagcgac tgccccgaca gaaatgatct
    17821 ccattcctca tggggcagcg gctgccccat gaggaatgat ccccgccgcc gcttccgcgg
    17881 cgacggggat ctcttccttg tcgtcagggg gagagggggc gggccccgag aggcggggcc
    17941 cgccgtccgt gcggcgtcag acctcgagaa aggtgacgcc cgtggcgctc tccgtcggga
    18001 aacgatgctc tcgcaagacc ccaactcaag gcactgttac ccatcctccc ggtccattct
    18061 gttacctatc ctcccggtct gtaccccgcc caccctccca gggagggagg caccacctcc
    18121 ccctcgggct ggaaggtatt cgaccctcaa gcaaaaaggg cgtggagccg cgaggcccca
    18181 cgcccttcct ggagggtcgg ctggaagacc ggccctagtt cttgcggttg cggcgccggt
    18241 tgttgcgcgg ccggtcggca ggacggttgt tccggccggt gcccggatcc tcggtgacct
    18301 gctggacgtt cagcgcccag aacaggttgc gcaccgcgat cgggtcgacc ttgaccacca
    18361 cctgcccggc ctggaaggtc gcgccgccgc cgctcttcgg gccgaagagg aacggataga
    18421 ggtcgaccgg cgggatctcc gcgtcgaact gctcgttgat ctcgtcgatg aagacgttgg
    18481 cgatgtaggc gtagccgaag ggcgtcgggt ggacgccgtc gtaggagaag atgccgccgg
    18541 tgaggaaggc gggggtgaag gtcatgccgc ccacccgcag gccggtggtc gccgcctggt
    18601 tcaggaaggc gttcatgtcc acgaacgccg cgcccacccg gctcgcctcg gcctggatca
    18661 cctgattgaa ggcggcaacc cgggcgttga tggtggcgat ctcggaggcc gacagcaccg
    18721 ccgcgtcgga cagcggaccc gtgccgccca gcgacggcgg caggccccgc ccctgcgcca
    18781 gctcggcgct gccggtcagc agcacccggt cacccgcgcc cagcgggccg ttggggccga
    18841 tcagcgggat cggctggccg ttgttgagca ccggctggtt ggtctgcggg ttcaccacca
    18901 ccgtcggcac cgtattgacg tacgggatca ccgtcacgtt cgggatgttg gcgatcgcca
    18961 tccgggcgcc cgtggcgttc agggcgttga cgatctgccg gtactcgtcc gtgaatgtgg
    19021 cgagcggcgt cagggtgttg tcgtcgacca cgccggcgag cgccgccgcc agcgcgtcgt
    19081 tgttgccgat ccacagggtg acgaaggtcg gctgcagtgc gaccgcctgc tggagctggg
    19141 tgcccagacc gcgcaggatc aggtcgttga ggccgccccg gacggtgaac agcacgtcgt
    19201 ggacgtcggc gcccggcacc gccaggttgt tgtacggccg ctgcagggtt gcattcagcg
    19261 gcgtgccggt gccggcggcc tggccgatcg acagcggcag caggctgcgg agctgctgca
    19321 gcgccggaat gcccggagcg ctgaccagcg gctgctcgaa gccggtggca cggccggtgg
    19381 cctgccggta gatcagcgcc ggatagctgt tcctctgcgc cgattcgacc aggccgccgc
    19441 tcatgaaggc cgcggtcagg ctgtcgccca cggccacgta ggtggtgaaa ttggccgcgc
    19501 cggtgtccac ctgggccgac gccggcagga ccgcggcaag gagggccgaa cccaggaggg
    19561 cggctctcag gacgttgcga aacatggatg tctcctcctt caaggggttg tcagggcctc
    19621 agcggcccac cgtcaggccg aacagccagg ccgtggtgtt gtaggtgccg aagaagtcgc
    19681 cctcgttctc gaagctgcgc gcccgggtgc gctcctcgaa catcaggtac atcagcgcga
    19741 cgtcgatcct ccagttgccg ctgtggcccc agccgagggt gatgccgtca cgattggcgt
    19801 ccggcagcag cggcgacacc gcctcctccg gctgcggcgt ctcgtcatag acgtagccga
    19861 ggcgccactg ggtggagggg ctcgccgtcc agctcaggcc ggcgcggtag ctgtagacgt
    19921 cgtcccaacg tgagacgatc tccgagtccg gcaggtcgtt gttgacgaag tcgatctcgg
    19981 tggtgtcgaa cgagctccag cccatgtagt tgaagtcggt gttgagccgc aggttcggcg
    20041 acagggcgaa cgacaggccg aggctcgcct ggtcggggaa ctcgatggag gcctccgccg
    20101 gcaggtcgcg gttgaacggc aggcgcgacg cgatgacctg gtcgagttgc gcgttgccgg
    20161 tcgagacctg ctccagccgc gcgtccccct cgtagtcgat ggtgagattg ctgcggtagg
    20221 agaggcccca ggagaagctg ttgttccacc ggtgcaggat gccgacgttc cagccgtacc
    20281 cctcgccgaa gtcggtctcc aggttgagcc ggccgacgtc gacgaagcgg aaggtgaacg
    20341 ggttggtggt gccgacgttg cggttcaggt cgacgtccga cacgcgggcg atggcgccga
    20401 tgccgaggcc gaagttgggg gtgagctgga tgccgatcgt cgggttcagg tcgacggaac
    20461 ggatcgccgc ctcggtgctg atgaaccgcc cggcgaactg gtcggggttc tcccacgagg
    20521 tggtcaggcc gaacggcgca tagagcccga agccgaactt cagccggtcg ttgatcggag
    20581 tcacgtaata ggcatggggc aggatctcgg ccagcgtctc ctgctcggcc cggtagcccg
    20641 gcccggggaa cgcccccgca ccctcgaact ccgcctcgct ggacgtgatg taggtcgcgc
    20701 ccaccatcca gtcggatttc tcgacgaacg ccaggccgcc ggcgttgtga tacagcatgg
    20761 aaggatcgtc cgcctgaccg gtaaaggccc ccgcggtgcc catcgcccgg gtgccctgct
    20821 cgaagatcga gaaaccggcc gcgtggaccg ggaccacggc caacactccc agcgcggcca
    20881 ctcccaaagc cctccggatc aactctcctc gcctgcgcat ttgttcatct cctcctgtcg
    20941 tttgaatgat cccggtgctc cgatgggtac tgaaaacctg atggaatctg cggatggaac
    21001 ggagcccggg tccaagactc ctgttcggat aacgctcaaa cggtagtccc aaccggagga
    21061 ggtgtcaacg gcggagagct ggtacagtac gccgcgcggg agggcccgca ggagggaaga
    21121 ggaagatgcc gctggaagac cggatccagg aagagacgtt cgagaagctc cacgagtatc
    21181 tgggtgagct gttcgaggac ccctatcacg atcccgagaa cgatcatttc tacgtgcgct
    21241 atggcaccac ggtgctggag atctcggtgg agccgtacga ctcggaggag gccgtggtca
    21301 tcatcatgtc ctactgcgtg caggacgtgg agctggcgga cgacctgctg ctcggcctgc
    21361 tggagctcaa ccaccaggtg ccctgcggcg ccttttccct ggtggacaac gacatcttct
    21421 tctcctactc gctgttcggc cgccatctgt cgccgcgcga cctgctgcgg gcgatcaccg
    21481 cggtggccac ggtggccgac gactacgacg accgcatcgt cgccaagtac ggcggccaga
    21541 ccgctctgga gcgcatccag gacaccggcg gccggcggcg gcggcagggc tacggcgagg
    21601 agtaggggat ggcggccggc ggccgcgtca gtagcggccg ccgctcttga ccgccgcctc
    21661 catctcgcgg tccagctccc gctccttggt ggcctggcgc ttgtcgaact gcttcttgcc
    21721 ctggaccagg gcgatctcca gcttgatcag gttgcccttc aggtagacgg agagcgggac
    21781 gacggtcagc cccttgagct gggtcttgcc gaagaggcgc tcgatctggc ggcgttgcag
    21841 cagcagcttg cggtcgcgct ccggcgg
//
LOCUS       DASPJI010000663         4584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_42963, whole genome shotgun sequence.
ACCESSION   DASPJI010000663 DASPJI010000000
VERSION     DASPJI010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42963"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VEL74_18870"
     CDS             complement(<1..437)
                     /locus_tag="VEL74_18870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG64650.1"
                     /translation="MSQPLLAARGVAWRSPDDRPVLGPLDLQVKRGECLVVVGPNGAG
                     KTTLMRVLTGLLRPTAGEVVLDGHGLATLSRRELARRVAYVPQIRPDRVPLDVERVVL
                     LGRYPHLSRLQVSPRREDFAAVDEALAVVGAEELRHRPVDELSG"
     gene            complement(434..1192)
                     /locus_tag="VEL74_18875"
     CDS             complement(434..1192)
                     /locus_tag="VEL74_18875"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HYG64651.1"
                     /translation="MAPNLTETLFALGLGDRVVGVDDYSTYPPEVRSLPRLGGVMDPN
                     LEAIVALEPDLALLAPGEQELGNRLESLGVDTLYLPNDGLADVARTFTEVANRCGVPE
                     EGRRLLAEWQEGLAPNPVPGSPQVLVSIGRRPGSLADVLVAGPGTFYDELLTRMGAVN
                     VFADAPTRYPQINLEEVVARGPDVILEVRSDALPPPSMAALTRDWARLPGLPAVRDGR
                     VSVLGGDYVVTPGPRLPRLYQEMREALLRGGDRP"
     gene            complement(1297..3135)
                     /locus_tag="VEL74_18880"
     CDS             complement(1297..3135)
                     /locus_tag="VEL74_18880"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYG64652.1"
                     /translation="MRLILLWIGILGGGIPVFAQSTQQGTPASPTGSAAPTFADEVVV
                     TASLDAEDRDQIPASATVIDAAEIQARQADTLSEVVSTVPGLMVAQSGSPGQQTSLFT
                     RGSESEQTLLLWNGIPLNDPYFGGANWQLVSTDGVARVEVVRGPFSALYGGNALGGVV
                     QVLTGAEQGGRITLEGGENGYARGGLASGWDFGRARVDVTGHLRRGEGVLDNDYFDSE
                     ELVARSLWTLSPGISLGVMARANDSETGIPLSGGIATPNRTISWQERELAVPFRADLG
                     RWEVEAQLSQTLFDSSYRDPDDPFGFTATDTESEGLRGRAVATWRVDDDLWIAAGTEA
                     ERLEVTDSSVFGTNLDGSRQRTWAVFGQGSWGRGPLRLEGGVRRDDNDVYGGQTSFRG
                     GAVVKLGGGTRLRVSYGEAFRAPSLGELFFPGSGNPDLQPETGESWELGLEHEAGGWR
                     FGLTGFDNRQRNLIDFDFVGFRNINVGRTRSRGVEAEAGYRRGVWSARLNGTYMDAED
                     EATGLPLLRRPERSANLVLTASPGPWALNLVGRYVGPRPDVDPITFGRAENEGFTRVD
                     LAARYEALSWLSPYARVENVAGEEYDEVLGFPSPGRTWIGGVAFDF"
     regulatory      complement(3244..3466)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3560..4090)
                     /locus_tag="VEL74_18885"
     CDS             complement(3560..4090)
                     /locus_tag="VEL74_18885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64653.1"
                     /translation="MARSLVRVWPSLAVLALLASPAAADIFYVTLNNGTVMESAYQPQ
                     ESSWDSSMVLLLTEVGNWIGVPKDQIEKVETSSQKSGFGVRINATTVAIGWSPNDAED
                     PEAAAAGADGQQPVSAAEARFQQAIQNLNQTISQVEAQRQSQENYSIDQFVEPGETQG
                     IPGALISPYSGGPSSQ"
     gene            complement(4137..>4584)
                     /locus_tag="VEL74_18890"
     CDS             complement(4137..>4584)
                     /locus_tag="VEL74_18890"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HYG64654.1"
                     /translation="RGGRFSGIAFTSERQLAGGGPCRLPWAADGDFQPTSRNPALWLE
                     PSGSIVWGVLGGEPNGALLWNTFPWHPWGAAGPLSNRTPERSLVAANRHVLERLLETV
                     GGARVLAVGRTAQAALAALGVEAPALRHPAHGGANLFREQFRAHTC"
BASE COUNT          636 a         1602 c         1733 g          613 t
ORIGIN      
        1 ccggacagct cgtcaaccgg ccggtgccgc agctcctcgg cgccgacaac ggccagcgcc
       61 tcgtcgacgg cggcgaagtc ctcccgccgg ggggagacct gcagcctcga gaggtggggg
      121 tagcgcccga ggagcaccac ccgttccacg tccagcggca cgcggtccgg ccggatctgc
      181 gggacgtagg cgacgcggcg cgccagctcg cgtcgcgaga gggtggcgag gccgtggccg
      241 tccaggacga cctcgccggc ggtcgggcgg agcaggccgg tgagcacccg catcagagtg
      301 gtcttgccgg cgccgttggg cccaaccact accaggcact cgccgcgctt gacttggaga
      361 tcgagcggcc ccagcaccgg gcggtcgtcc ggcgaccgcc aggcgacgcc gcgggcggcg
      421 aggagcggct ggctcacggc cgatcccccc cgcgcagcag cgcctcgcgc atctcctgat
      481 agagccgggg gaggcgcggc cccggggtga cgacgtagtc cccgccgagg acggagacgc
      541 ggccgtcgcg caccgccggc aatcccggca ggcgcgccca gtcgcgggtc agcgccgcca
      601 tcgacggcgg cggcagcgcg tccgagcgga cctcgaggat gacgtccggc ccccgcgcca
      661 ccacctcttc gaggttgatc tgcggatagc gggtcggcgc gtcggcgaag acgttgaccg
      721 cccccatccg ggtgagtagc tcgtcgtaga acgtccccgg gccggccacc aggacgtccg
      781 ccaggctccc cggccggcgg ccgatggaga ccagcacctg gggagagccc ggcaccgggt
      841 tcggcgccag cccctcctgc cactcggcga gcaggcgccg cccctcctcc ggcacgccgc
      901 agcggttcgc cacctcggtg aaggtgcgcg ccacgtcggc caggccgtcg ttggggagat
      961 agagggtgtc gaccccgagg ctctcgagac ggttcccgag ctcctgctcg ccgggagcga
     1021 ggagcgccag gtccggctcg agcgccacga tagcctccag gttggggtcc atcacccccc
     1081 cgagccgcgg cagcgaccgc acctcggggg gataggtgct gtagtcgtcg acccccacca
     1141 cccggtcccc caacccgagg gcgaagaggg tctcggtgag gttgggcgcc atggagacga
     1201 tgcgccgcgg cgcccgcggc gcgggcgcct cctcccggca ccccgtgagg atggccggaa
     1261 ggaggagcgc tggcaggagg gccgggagga ccggcctcag aagtcgaatg ccacccctcc
     1321 gatccaggtg cggcccgggc tggggaagcc gagcacctcg tcgtactcct ctccagccac
     1381 gttctccacc ctggcatagg gggagagcca ggacagcgcc tcgtagcgcg ccgccaggtc
     1441 gacacgggtg aagccctcgt tctcggcccg tccgaaggtg atcgggtcga cgtcggggcg
     1501 cgggccgacg tagcgcccca ccaggttgag ggcccagggg ccggggctgg cggtgagcac
     1561 caggttggcg ctccgctccg gccggcgcag cagcggcagg ccggtcgcct cgtcctcggc
     1621 gtccatgtag gtgccgttca ggcgggcgga ccagacgcca cgccggtagc cggcctcggc
     1681 ctccaccccg cggctgcggg tgcggccgac gttgatgttc cggaagccga cgaagtcgaa
     1741 gtcgatgagg ttgcgctggc ggttgtcgaa accggtgaga ccgaagcgcc agccgccggc
     1801 ctcgtgctcg aggcccagct cccagctctc gccggtctcc ggctgcaggt ccgggttgcc
     1861 gctgccgggg aagaacagct cgccgaggga gggggcgcgg aacgcctcgc cgtagctcac
     1921 ccgcagccgg gtgccgccgc cgagcttgac gacggcgccg ccccggaagc tggtctggcc
     1981 accgtagacg tcgttgtcgt cgcggcgcac gccgccctcg aggcgcagcg ggccgcgccc
     2041 ccagctcccc tggccgaaca ccgcccaggt gcgctggcgg ctgccgtcca ggttggtgcc
     2101 gaacaccgac gagtcggtca cctccaggcg ctcggcctcg gtaccggcgg cgatccagag
     2161 atcgtcatcc acccgccagg tggccacggc ccggccgcgg agcccctccg actcggtgtc
     2221 ggtggcggtg aagccgaacg ggtcgtcggg gtcgcggtag gaggagtcga agagggtctg
     2281 cgagagctgg gcctcgacct cccaccgtcc gaggtcggca cggaagggga ccgccagctc
     2341 gcgctcctgc caggagatgg tgcggttggg cgtcgcgatc ccgccggaga gggggatgcc
     2401 ggtctccgaa tcgttggccc gcgccatgac gcccaggctg atgccgggag agagggtcca
     2461 gagggagcgg gcgaccagct cctcgctgtc gaagtagtcg ttgtcgagca ccccctcgcc
     2521 gcgccgcagg tggccggtga cgtcgacgcg ggcgcggccg aagtcccagc cggaggcgag
     2581 gccgccgcgg gcatagccgt tctccccccc ttcgagggtg atccggccgc cctgctcggc
     2641 gccggtcagc acctggacga cgccgcccag cgcgttgccg ccgtagaggg cgctgaacgg
     2701 gccgcgcacc acctcgacgc gggcgacgcc gtcggtggag acgagctgcc agttggcgcc
     2761 gccgaagtag ggatcgttga gcgggatgcc gttccacagg agcagggtct gctcggactc
     2821 ggagccgcgg gtgaacaggg aggtctgctg cccgggcgag ccggactggg cgaccatgag
     2881 cccggggacg gtggagacca cctcggagag ggtgtcggcc tggcgggcct ggatctccgc
     2941 ggcgtcgatg acggtggccg aggcggggat ctggtcgcgg tcctcggcgt cgagagaggc
     3001 ggtgaccacc acctcgtcgg cgaacgtggg ggcggcgctg ccggtggggg atgccggagt
     3061 gccctgctgg gtactctggg cgaagaccgg gattccgccc ccgaggatcc cgatccacag
     3121 aaggatgagg cgcatgtcgt gctccttcgc tggaaagcgc gacgccccac cgggaagggt
     3181 ggggcgtggg agcaggggac atgcggcggg gcatggccga cgcgcccgca cccgcacccc
     3241 gcgggaggcg tcggacggtt cggcaggtct cctggcttcc ggatctccct actcccgggc
     3301 cttcccacac gagcaaccac gatggtgatc cacccgtgca gtggcgtact tccggtttcg
     3361 ttcccggtta cagtggcggg ggccgcgccg gatttgcacc ggcttccctt tgaagacgcc
     3421 gcctcccctg gggaggcggg atgtcaccgc accgtcgagt ctgtcaaaga ggagccctcc
     3481 cacggcggga gggctcggag gatcagtcta gctcggggag aggccggttg gctactgccg
     3541 gccggctatt gccgggtggc tactgggacg aggggccgcc ggagtagggg ctgatcagcg
     3601 cccccgggat gccctgggtc tcgccgggct cgacgaactg gtcgatgctg tagttttcct
     3661 gcgactggcg ctgggcctcg acctggctga tcgtctggtt caggttctgg atggcctgct
     3721 ggaagcgggc ctcagcggcc gagaccggct gctgcccgtc ggcgcccgcg gcggccgcct
     3781 ccgggtcctc ggcgtcgttc ggcgaccagc cgatggcgac ggtggtggcg ttgatccgca
     3841 cgccgaagcc gctcttctgg ctgctggtct ccaccttctc gatctggtcc ttggggaccc
     3901 cgatccagtt gccgacctcg gtcagcagca ggaccatcga ggaatcccag ctcgactcct
     3961 gcggctggta ggcggactcc atgacggtgc cgttgttgag cgtcacatag aagatgtcgg
     4021 ccgccgccgg cgaggcgagc agcgcgagga cggcgagaga cggccagaca cgaaccaggg
     4081 accgcgccat ggaagacctc ctgcggcaga gaccgcattg aatgactgct atgagtctag
     4141 cacgtgtggg cgcgaaattg ctctctgaag aggttggccc cgccgtgcgc cggatggcgg
     4201 agcgccggcg cctcgacacc gagggcggcc agggcggcct gggcggtccg tccgacggcc
     4261 aggacgcggg cgccgccgac cgtctccagc agccgctcga gcacatgccg gttggcggcg
     4321 acgaggctcc gctcgggcgt cctgttgctg agcgggccgg ccgctcccca ggggtgccag
     4381 gggaaggtgt tccataagag ggccccgttc ggctctccgc ccaggacgcc ccagacgatg
     4441 ctcccggacg gctcgagcca gagcgccggg ttgcggctgg tgggctggaa gtcgccgtcc
     4501 gccgcccacg gcagccggca gggtccgccg ccggcgagct ggcgctcgct ggtgaacgcg
     4561 atccccgaga agcgcccgcc gcgg
//
LOCUS       DASPJI010000664         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_43014, whole genome shotgun sequence.
ACCESSION   DASPJI010000664 DASPJI010000000
VERSION     DASPJI010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43014"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2304
                     /locus_tag="VEL74_18895"
     CDS             <1..2304
                     /locus_tag="VEL74_18895"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYG64655.1"
                     /translation="GKIEVLAEFFGGQGTINVPCWSPDGRKIAFISDRTGEEEVWLVD
                     QDGGSNTDKGASQPEQLTRGGKAMRYAPEWAPDGKRIAFGDKDGKLYVLTVADRKLVE
                     VADERHGTMQDYVWSPDGGHLALSLADDNLTRSLWIWSVSDGKLRRVTDERFDEFNPT
                     WDPQGKYLFYLSNRDYSPQADFFDYNYAIVRNTGIYAMALRRDVGHPFPPESDEVTLS
                     EEEKKDEAEKKEGAEGKDAVKPASAVRIDFDGLSGRVARVPVPFGNYSGLAAKDGHLL
                     YFRGGAFFFDGSSDDTVLQIFSMKDRKETTLAEKVDGYSLSQDGGKVLIVKEGGHHLL
                     DATPGGKDSGKPISTAGLVVDRVPAEEWAEVFDEAWRRYRDFFYVENMHGYDWEALRE
                     QYRPLLAHVGHRSDLNYILGEMIGELNISHAYVAGGDFQLPPRPRVALPGARFELDKA
                     AGRYRVTRIFSGENEEDLYRSPLTELGVDVRPGDYVLAVDGIDLPANDNPYRLLRHKS
                     GQPVRLTVNSRPSYEGAREAIFQPVSSEANLVYLQWVKANRERVDKLSGGRVGYLHIP
                     DMGGDGLREFIKAFYPQLRKEGLVIDVRSNGGGFVSAMILDRLQRVLRSVDFSRLDDS
                     ARTYPPSVFHGHMVCLMNETTSSDGDVFAAMFKQAGLGPLIGKRTWGGVVGITGHGPM
                     IDGGQISVPESGSASPDGQWIIEGHGVDPDIEVDNDPKSLLAGRDPQLERGVAEVLKA
                     IEKEPRKLPARPAPPVKTQAGMAGSGG"
     gene            complement(2441..>2778)
                     /locus_tag="VEL74_18900"
     CDS             complement(2441..>2778)
                     /locus_tag="VEL74_18900"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HYG64656.1"
                     /translation="PTILQLTGTSASRKLDGRSLIELAQNPDAGKDRTLLVEGFSRNR
                     NQVTYAGVRTDRWLYVEYQNGGRELYDLERDPRQLQSRHADPALANVRADLAGRLGRL
                     RTCAGEGCQ"
BASE COUNT          456 a          945 c          968 g          409 t
ORIGIN      
        1 gggaagatcg aggtgctggc ggagttcttc ggcgggcagg ggacgatcaa cgtgccctgc
       61 tggtcgccgg acggccgcaa gatcgccttc atctccgacc gcaccggcga ggaggaggtc
      121 tggctcgtcg accaggacgg cggctccaac acggacaagg gggcgagcca gcccgagcag
      181 ctcacccgcg gcggcaaggc catgcgctac gcgccggagt gggcccccga cggcaagcgc
      241 atcgcgttcg gtgacaagga cggcaagctc tacgtcctca ccgtcgccga ccgcaagctc
      301 gtcgaggtgg cggacgagcg gcacggcacc atgcaggact acgtctggtc ccccgacggc
      361 gggcatctcg ccctcagcct ggcggacgac aacctcacgc gctccctgtg gatctggagc
      421 gtgagcgatg gaaagctccg gcgggtcacc gatgagcggt tcgacgagtt caaccccacc
      481 tgggaccctc aggggaagta cctcttctac ctgagcaacc gggactactc cccgcaggcc
      541 gacttcttcg actacaacta cgccattgtc cgcaacaccg gcatctatgc catggccctg
      601 cgccgggacg tgggtcaccc ttttccgccc gagagcgacg aggtcaccct cagcgaggag
      661 gagaagaagg acgaggcgga gaagaaggaa ggcgcggagg ggaaggacgc ggtgaagccg
      721 gcttcggccg tccgcatcga cttcgacggc ctctccggcc gcgtggcccg cgtgcccgtc
      781 cccttcggca actacagcgg cctggccgcc aaggacgggc atctgctgta cttccgggga
      841 ggagccttct tcttcgacgg cagctccgac gacacggtcc tccagatctt ctccatgaag
      901 gaccgcaagg agaccaccct cgccgagaag gtggacggct actccctgtc gcaggacggc
      961 ggcaaggtgc tgatcgtgaa ggagggcggc caccatctcc tggatgccac tcccggcggg
     1021 aaggactccg gcaagccgat ttcaaccgcc gggctggtgg tggaccgggt gccggccgag
     1081 gagtgggccg aggtcttcga cgaggcctgg cgccgctacc gtgacttctt ctatgtcgag
     1141 aacatgcacg gctacgactg ggaggcgctg cgcgagcagt accggcccct cctcgcgcac
     1201 gtcgggcacc gctcggacct gaactacatc ctcggcgaga tgatcggcga gctcaacatc
     1261 tcccacgcct atgtcgccgg gggcgacttc cagctcccgc cgcgcccccg ggtggccctg
     1321 cccggcgccc gcttcgagct ggacaaggcc gccggccgct accgcgtcac ccggatcttc
     1381 agcggcgaga acgaggagga cctctaccgt tcgccgctga ccgagctggg ggtggacgtc
     1441 cgtccgggtg actacgtgct ggcggtcgac ggcatcgatc tgccggcgaa cgacaacccc
     1501 taccggttgc tgcgccacaa gtcgggccag ccggttcggc tcacggtcaa cagccggccc
     1561 agctatgaag gggcacgcga ggcgatcttc cagccggtct ccagcgaggc caacctggtc
     1621 tacctccagt gggtgaaggc caaccgcgag cgggtggaca agctgagcgg cggccgggtc
     1681 ggctacctcc acatccccga catggggggc gacggcctgc gggagttcat caaggcgttc
     1741 tatccccagc tccgcaagga gggtctggtg atcgacgtcc gcagcaacgg cggcggcttc
     1801 gtgtccgcca tgatcctgga ccggctgcag cgggtcctgc ggtcggtgga cttctcccgg
     1861 ctggacgact ccgcccggac ctatccccct tcagtcttcc acggccacat ggtctgcctg
     1921 atgaacgaga ccacctcgtc cgacggcgac gtcttcgccg ccatgttcaa gcaggcgggc
     1981 ctcggtcccc tgatcggcaa gcgcacctgg ggtggcgtcg tcggcatcac cggtcacggc
     2041 ccgatgatcg acggcggcca gatcagcgtc ccggagtctg gctccgccag tcccgacggc
     2101 cagtggatca tcgaagggca cggggtcgac ccggacatcg aggtcgacaa cgacccgaag
     2161 tcgctcctgg ccggccgcga cccgcagctc gagcgcgggg tggcggaggt gttgaaggcg
     2221 atcgagaagg agccccgcaa gctccccgcc cgtcctgcgc cgccggtcaa gacccaggcc
     2281 gggatggcag ggagcggagg gtagggaggc agggaaaaaa cacggacgac acggaccagc
     2341 acggatcgcg ggagcaaggg ggaggtggtg cctcccccct tgggcgggtg ggtgggtagg
     2401 aagcggggga ggtcgaggag ggggtgtttg agcctctcct ctactggcac ccctcccccg
     2461 cgcacgtccg cagccggccc aggcggccgg ccaggtccgc tcggacgttc gccagggcgg
     2521 ggtcggcgtg acggctctgg agctgccggg ggtcccgctc caggtcgtag agctcccggc
     2581 cgccgttctg gtactcgacg tacagccagc ggtccgtccg caccccggcg taggtgacct
     2641 ggttgcggtt gcggctgaag ccctcgacga ggagcgtccg gtccttcccc gcgtccggat
     2701 tctgggccag ctcgatcagc gagcggccgt ccagcttccg gctggcactg gtgccggtga
     2761 gctgcaggat cgtcggcg
//
LOCUS       DASPJI010000665         6215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_43074, whole genome shotgun sequence.
ACCESSION   DASPJI010000665 DASPJI010000000
VERSION     DASPJI010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43074"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2472
                     /locus_tag="VEL74_18905"
     CDS             <1..2472
                     /locus_tag="VEL74_18905"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HYG64657.1"
                     /translation="LEEFYRARGFFGAQVLIRVRADPASGGTALTYLIQSGPRATIST
                     VVFQGETAPFTPAQLVEQLEQRPGRPYDQSTLREDVDRLQTWLVQQEHRAADVRPPTE
                     SIDAAANTVALTYPIEVGPRVTAVVEGADLEDLRRRNLLPFLGEQGFDEALVTQAAGR
                     VRRYYQEQGHYRVQVESRIEEEGDELRVVLTVAPGPVYTLEEVRFTGNETFGAESFTD
                     LIATAPRGALARFGLGSRGRLVTEELDADLENILAFYRWEGFPAAEIGPARVEEQGSE
                     LRVEIPIREGLRQVVETLELRGVQAVDPRSLSLPLEPGGPFSPSRLDQALARLRQAYV
                     DRGYAGAQVSARQEWDEGRSRVGVIIEALEGPRILLDRVIVRGNQRTDSDVIRRSIAL
                     RPGDPVSQARVYEIERDLYRLGIFSSVDAELTLAGIEASTRDLIVRVREGLPRRVAYG
                     LGFEYSREDDDYGPRGSVSFTHNNVAGRALTLRGDLRVSSRLGDGGDGIDVSSRLLLG
                     QPYLGRYPVPTTYSLFYFDENRDFGNVTRKGARVESHRDFGDRRVGLVYDYRLVELTD
                     AEDPFRAEPEDQDLQISSLVPSFFWDRRDDPLLATRGWSSLAQLQYAFPFLQAEGEFL
                     KLFVQQTQYLPLNGRPPAGLEQVVVASLRAGGIEPFDTLRPELSFPLQLRRQGLPSAD
                     VFIDERFFAGGATTHRAYGLDDLGIRGETLILRPGEDRDPGNPRNYYSSGGNGLLLFN
                     LEYRFPLFGPVFGGSVFYDAGNVWPDWRDIDTGDIKQGIGVGLRYNSPIGPIRIDVGY
                     KLDPDPGEEDSENRYQFNFTFGNPF"
     gene            2612..3814
                     /locus_tag="VEL74_18910"
     CDS             2612..3814
                     /locus_tag="VEL74_18910"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HYG64658.1"
                     /translation="MPFASIEAAIEEFRQGRFVIIVDDEDRENEGDLAIAAEKVTPAA
                     VNFMAKQGRGLICLAMTEERCDELDLPLMVQSNTSPFGTAFTVSIEARGKISTGISAA
                     DRAATILTAIDPDTRPSDLLRPGHVFPLRAKKGGVLKRAGQTEASVDLAALAGLSPAG
                     VICEIMNDDGTMARVPDLTKFAAEHGLLMITVADLISYRLRHETLVKKVASPRLPTLY
                     GDFRIHAYRSDVTGDENVALVMGEIDPEEPVLVRVHSQCLTGDIFGSARCDCGPQLEQ
                     ALVTIAEEGRGVLLYLLQEGRGIGLMNKLRAYELQEQGHDTVEANERLGFRPDQRDYG
                     IGAQILRDIGVRKMRLLTNNPSKYIGLNGYGLEIVERVPLEVPPTESSRAYLKTKREK
                     LGHLLKLV"
     gene            3833..5086
                     /locus_tag="VEL74_18915"
     CDS             3833..5086
                     /locus_tag="VEL74_18915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYG64659.1"
                     /translation="MKILLVTSRYPWPARRGDQMRALQMLDFLSGEHEVTLLAPAPGP
                     DLPKPPAAVAYRIEHYQPERGVAFVQGMSRAVLERLPLQTGLFYQPDLGRKLRELAPK
                     ADLGILQLVRLASHLEDFGKTPILVDLIDSLSLNFSLRADVDRLWLRPALRLEARLLA
                     RAERRLCEHAAGMIVVCERDRQALVNRLPAALAAKIGVVRLAVRERQFAPPPEGEQLR
                     APGDEGPILAMTGNLGYFVNVDAVTWWLRDVWPTLRHVRPDVRVVVAGDRPARAVRRA
                     VETAGVRLLESPRDMRSVISQATLSLAPMRCGSGVPIKVLEAWAVGVPVVASPWAVAG
                     TSGRQGEDFRLVGQHPVEWVTAVHELLEDPAARERLATSGRQRLAADYSRDLARRQLL
                     GVIQSLGLAAAPGVSAAPLPRLAAE"
     gene            5145..5405
                     /locus_tag="VEL74_18920"
     CDS             5145..5405
                     /locus_tag="VEL74_18920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HYG64660.1"
                     /translation="MPAPSKISVVTERGQVSIPAELRKELALEKGRRLLWEKVGDSEL
                     RVVILPEPERRGAMAMLGFARRLDPTRRRTEDWMEELREGES"
     gene            5405..5824
                     /locus_tag="VEL74_18925"
     CDS             5405..5824
                     /locus_tag="VEL74_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG64661.1"
                     /translation="MAWVVDTCILIDVFEDDPRFGRHSALTLDACADQGLVLCPVTYT
                     EMAPAFRGDLGLQDEFLTGIGIDFRQEWSWADTLRAHRAWHDFIRKKRSRELPKRPLA
                     DILIGAFATRHDGLITRNPADFAAVFPDLALRDLFRE"
BASE COUNT          911 a         2301 c         2101 g          902 t
ORIGIN      
        1 ctcgaggagt tctaccgcgc ccgcggcttt ttcggcgccc aggtgctgat ccgcgtccgc
       61 gccgacccgg cgtcgggggg cacggcgctc acctacctca tccagagcgg cccccgggcc
      121 accatctcga cggtcgtctt ccagggggag accgcgccgt tcacccccgc gcagctcgtc
      181 gagcagctcg agcagcggcc cggtcgcccc tacgaccaga gcaccctgcg cgaagacgtc
      241 gaccgcctcc agacctggct cgtccagcag gagcaccgcg ccgccgacgt ccggcccccc
      301 acggagagca tcgacgccgc ggccaacacg gtcgccctca cctatcccat cgaggtcggg
      361 ccgcgggtga ccgctgtcgt cgaaggggcc gatctggagg atctgcgccg gcgcaacctc
      421 ctccccttcc tcggcgagca gggcttcgac gaggcgctgg tcacccaggc ggccggccgc
      481 gtccgccgct actatcagga gcaggggcac tatcgcgtcc aggtcgagtc gcggatcgag
      541 gaggaggggg acgagctgcg ggtggtcctc accgtcgcgc cggggccggt ctacaccctg
      601 gaggaggtcc gcttcaccgg caacgagacc ttcggcgccg aaagcttcac cgacctcatc
      661 gccaccgccc cgcgcggcgc gctcgcccgc ttcggcctgg gcagccgcgg ccgcctggtg
      721 accgaggagc tcgacgccga cctcgagaac atcctcgcct tctaccgctg ggaggggttc
      781 cccgcggccg agatcggccc cgcgcgggtg gaggagcagg ggtcggagct gcgggtcgag
      841 atccctatcc gggaggggct ccggcaggtg gtcgagaccc tcgagctccg gggtgtccag
      901 gcggtcgatc cccggagcct gagcctccct ctggagcccg gcgggccgtt cagcccctcg
      961 cgcctcgacc aggccctggc acggctgcgc caggcctacg tcgaccgggg gtatgccggg
     1021 gcccaggtgt cggcgcgcca ggaatgggac gaggggcggt cacgggtcgg cgtcatcatc
     1081 gaggcgctgg aggggccgcg catcctcctc gaccgggtga tcgtgcgcgg caaccagcgc
     1141 accgacagcg acgtcatccg gcgcagcatc gcgctgcgcc cgggcgaccc ggtgagccag
     1201 gctcgggtct atgagatcga gcgcgacctc taccgtctcg gcatcttctc cagcgtcgac
     1261 gccgagctca ccctggccgg catcgaggcc tcgacccgcg acctcatcgt gcgggtgcgg
     1321 gaggggctgc cgcggcgggt ggcctacggc ctcggcttcg agtacagccg cgaggacgac
     1381 gactacggcc cgcgggggag cgtcagcttc acccacaaca acgtggccgg ccgggccctc
     1441 accctgcgcg gcgacctgcg ggtgagcagc cgcctcgggg acggggggga cgggatcgac
     1501 gtcagctccc ggctgctcct cgggcagccc tacctggggc gctacccggt ccccaccacc
     1561 tactcgctct tctacttcga tgagaaccgg gacttcggca acgtcacccg gaagggggcg
     1621 cgcgtcgagt cgcaccgcga cttcggcgac cggcgcgtcg gcctggtcta tgactaccgg
     1681 ctggtcgagc tgaccgacgc cgaggacccc ttccgggcgg agcccgagga ccaggacctc
     1741 cagatctcca gcctggtgcc cagcttcttc tgggaccgcc gcgacgaccc gctgctggcg
     1801 acgcgcggct ggagctcgct cgcccagctc cagtacgcct tcccgttcct ccaggccgag
     1861 ggggagttcc tcaagctgtt cgtccagcag acgcagtacc tgccgctcaa cggccggccg
     1921 cccgcgggcc tggagcaggt ggtggtggcg agcctgcggg ccggcggcat cgagccgttc
     1981 gacaccctgc ggccggagct gtccttcccg ctccagctcc ggcgccaggg gctccccagc
     2041 gccgacgtct tcatcgacga gcgcttcttc gccggcggcg ccaccaccca ccgcgcctac
     2101 ggcctggacg acctgggcat ccgcggcgag acgctcatcc tgcgccccgg cgaggaccgg
     2161 gaccccggga atccgcgcaa ctactattcg tccggcggca acggcctgct gctgttcaac
     2221 ctggagtacc gtttccccct cttcggcccc gtcttcggcg gctcggtctt ctacgacgcc
     2281 ggcaacgtct ggccggactg gcgcgacatc gacaccggcg acatcaagca ggggatcggc
     2341 gtcggcctgc gctacaactc gcccatcggc cccatccgca tcgacgtcgg ctacaagctc
     2401 gaccccgacc cgggcgagga agacagcgag aaccggtacc agttcaactt caccttcggc
     2461 aacccgttct aggccctgag ggccggttga cctttgccgc agggagacgt aaactgttga
     2521 cgggccgaca gctatcctcc cttccggggc ccgcagcggc tctcccgaac tgaaaaggcc
     2581 tgtgtccacc atgtccaaga cagcccaacc catgccgttc gcctccatcg aggcggccat
     2641 cgaggagttc cgccagggcc gcttcgtcat catcgtcgac gacgaggacc gcgagaacga
     2701 gggggacctc gccatcgccg ccgagaaggt cacccccgcg gccgtcaact tcatggccaa
     2761 gcaggggcgc ggcctgatct gcctcgccat gaccgaggag cgctgcgacg agctggacct
     2821 gcccctgatg gtgcagtcca acacctcgcc gttcggcacc gccttcaccg tctccatcga
     2881 ggcgcgcggc aagatcagca ccggcatctc ggccgccgac cgcgccgcca ccatcctgac
     2941 ggccatcgac ccggacaccc gcccgtccga cctgctgcgc ccggggcacg tcttcccgct
     3001 gcgcgccaag aagggcggag tgctcaagcg cgccggccag accgaggcgt cggtcgacct
     3061 cgccgccctc gccgggctct ccccggccgg cgtcatctgc gagatcatga acgacgacgg
     3121 caccatggcg cgcgtgcccg acctcaccaa gttcgccgcc gagcacggcc tgctgatgat
     3181 caccgtcgcg gacctcatca gctaccggct gcgccacgag acgctggtca agaaggtcgc
     3241 ctcgccgcgc ctgcccaccc tctacggcga cttccgcatc cacgcctacc ggtcggacgt
     3301 caccggcgac gagaacgtcg ccctggtgat gggggagatc gatcccgagg agccggtcct
     3361 ggtgcgcgtc cacagccagt gcctgaccgg cgacatcttc ggctcggcgc gctgcgactg
     3421 cggcccgcag ctcgagcagg cgctggtcac catcgccgag gaggggcgcg gcgtcctcct
     3481 ctatctcctc caggagggac gcggcatcgg cctgatgaac aagctgcgcg cctacgagct
     3541 ccaggagcag gggcacgaca ccgtcgaggc caacgagcgg ctcggcttcc ggcccgacca
     3601 gcgcgactac ggcatcggcg cccagatcct gcgcgacatc ggcgtccgca agatgcggct
     3661 gctgaccaac aaccccagca agtacatcgg cctcaacggc tacggcctgg agatcgtcga
     3721 gcgcgtgccg ctcgaggtgc cgcccaccga gagcagccgg gcttacctca agaccaagcg
     3781 cgagaagctc ggccacctcc tcaagctcgt ctgaggcggc cgggcgcttc ccttgaagat
     3841 cctcctcgtc acctcgcgct acccctggcc ggcccggcgc ggcgaccaga tgcgggcctt
     3901 gcagatgctc gacttcctct cgggggagca cgaggtgacg ctgctcgcgc cggcgccggg
     3961 gcccgacctg ccgaagccgc cggccgcggt cgcctaccgc atcgagcact accagccgga
     4021 gcgcggcgtc gccttcgtcc aggggatgtc gcgcgccgtg ctcgagcggc tgcccctcca
     4081 gaccgggctc ttctaccagc cggacctcgg ccgcaagctg cgcgagctcg cccccaaggc
     4141 ggacctcggc atcctgcagc tcgtgcgcct cgcctcccac ctggaggact tcggcaagac
     4201 gccgatcctg gtggacctga tcgacagcct gtcgctcaac ttctcgctgc gcgccgacgt
     4261 cgaccggctc tggctgcggc cggcgctccg cctggaggcg cggctcctgg cgcgggccga
     4321 gcggcgcctc tgcgagcacg ccgccggcat gatcgtggtc tgcgagcgag accgccaggc
     4381 gctggtcaac cgcctgccgg cggccctcgc ggcgaagatc ggcgtggtgc gcctggcggt
     4441 gcgcgagcgc cagttcgccc cgccgcccga aggggagcag ctccgcgccc ccggcgacga
     4501 gggcccgatc ctggcgatga ccggcaacct cggctacttc gtcaacgtcg acgccgtcac
     4561 ctggtggctg cgcgacgtct ggccgacgct gcgccacgtc cgccccgacg tgcgggtggt
     4621 ggtggcgggg gaccggccgg cgcgggcggt gcggcgggcg gtggagacgg ccggggtgcg
     4681 gctgctggag tcgccgcgcg acatgcgctc ggtcatctcg caggcgacgc tgtcgctcgc
     4741 gccgatgcgc tgcggctcgg gggtgccgat caaggtgctg gaagcctggg cggtgggggt
     4801 gccggtggtg gcctccccct gggcggtggc cggcacctcg ggacgccagg gggaggattt
     4861 ccggctcgtc ggccagcacc cggtcgaatg ggtgaccgcc gtccacgagc tgctggagga
     4921 ccccgccgcc cgtgagcgcc tggccaccag cggccggcag cgcctcgccg ccgactactc
     4981 gcgcgacctg gcgcgccgcc agctcctcgg cgtcattcag tccctggggc tcgccgccgc
     5041 cccgggcgtc tccgccgccc ccctcccccg cctcgcggcc gagtagccgc cgcgcccggc
     5101 agggggagct cctgcatgat ggtcttacat gataagatcc catcatgccc gctccctcca
     5161 agatctccgt cgtcaccgaa cgtggacagg tctcgattcc cgccgagctg cgcaaagagc
     5221 tcgccctcga aaaaggccgc cgcctgctct gggagaaggt gggcgacagc gagctgcgag
     5281 tcgtgatcct gccggagccc gagcgccgcg gcgcgatggc aatgctcggc tttgctcggc
     5341 ggctcgatcc cacccgccgc cggaccgagg actggatgga ggagctgaga gaaggcgaat
     5401 cctgatggcc tgggtcgtcg atacctgcat tctcatcgac gtcttcgagg atgacccccg
     5461 gttcggcagg cactccgccc tgacgctgga tgcctgcgcc gatcagggac tcgtcctctg
     5521 cccggtgacc tacacggaga tggcccccgc gtttcggggc gacctggggc tccaggacga
     5581 gttcctcacc gggatcggca tcgacttccg gcaggagtgg tcgtgggccg acacgctccg
     5641 cgcccaccgg gcctggcacg acttcatccg caagaagcgc tcacgagagc tgccgaagag
     5701 gcctctcgcc gacatcctca tcggcgcctt cgccacccgc cacgatggcc tgatcacccg
     5761 caatcccgcc gacttcgccg cggtcttccc ggatctcgcg ctgagggatc tgttccgcga
     5821 gtagctttcc tgtcgggaat gacgagggtc cgtcgggttg accctggaaa cttgtcggga
     5881 aaggcccctc atgatagcct ccgcggcgcc tcgatgtcgg gagttgctgg tgaattgtcg
     5941 ggaatgagcc cgaattcccg acagatgaag agggtcgaac ggctggtgaa cagggtcgag
     6001 gcgttcgacc ctgttcattt gtcaggaatg aacagggtcg catcgccttc gagcccggtt
     6061 tcccgacaaa cggacggcaa gatgcgggga gagcaggcac gggaggagtc gtgaggacga
     6121 aatcggcgtt gacggtccaa gaggctttcg cgcggctgga ggcgcaggcg gcatcgctcc
     6181 aggagagggc ggcctttcat cgcgtgcagg aggag
//
LOCUS       DASPJI010000666         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_389748, whole genome shotgun sequence.
ACCESSION   DASPJI010000666 DASPJI010000000
VERSION     DASPJI010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389748"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VEL74_18930"
     CDS             <1..1138
                     /locus_tag="VEL74_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64662.1"
                     /translation="NYGWRVMEGTSCLGRGSCPASVPGCNSPAYTGPLVEYDHTGGNC
                     SVTGGYVYRGGAIANLQGTYFFGDFCSGTVWTHRRLSGTSSEIRRLEDRVQQLVTFGE
                     DSQGEIYVAALNGAIHRVTGEAAPPPPPPPSSDRVGVYEPATARFHLKDRLITGPADR
                     VVRFGRGNSPWRPLAGDWNGDGTDTIGLYDAPRGLFRLKNTLTGGNADVLLRLRLPAG
                     PNQNAIPVAGDWDGDGRDGVGLFGSATTFYLKDTVDGGDFDAVCGFDLLGRPPAQYLP
                     IAGDWDGDGRDSIGLYRPATGTFLLADGCADQEPELEFQFGSGGRALPLAGDWDGDGR
                     DGIGLYDPRNGAFALKNEPGGGAADVSFRIRPRPRNGAPLAGAW"
     gene            1190..>2101
                     /locus_tag="VEL74_18935"
     CDS             1190..>2101
                     /locus_tag="VEL74_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HYG64663.1"
                     /translation="MLAGLGALGLALLAAAPATPALAAETYDLILRRGTIYDGGGGKP
                     FTGDVAVRGDRVAAVGDLGDAKGKVEVDAAGLAVSPGFINMLSWATESLIVDGRGQSD
                     IRQGVTLEVMGEGWSMGPVNAAMKKEALEEQGDVKFDIPWTTLGEYLDHLVKRGIAPN
                     VASFVGATTLRVHELGYENRPPKPEELARMRKLVAQAMEEGALGVGSSLIYAPAFYAK
                     TDELVALCEEAGRYGGMYISHMRSEGNRLVEAVDELITIARRAKVPAEIYHLKAAGKS
                     NWGKLDQVIRKVEEARASGLRITADMYN"
BASE COUNT          316 a          742 c          759 g          284 t
ORIGIN      
        1 caactacggc tggcgggtga tggagggcac ctcctgcctc ggccgcggct cctgcccggc
       61 gtcggtgccc ggctgcaact cgccggccta caccgggccg ctcgtcgagt acgaccacac
      121 cggcggcaac tgctcggtca ccggcggcta cgtgtaccgc ggcggcgcca tcgccaatct
      181 gcagggcacc tacttcttcg gcgatttctg cagcggcacg gtctggaccc accggcgcct
      241 ctccggcacc tcctccgaga tccgccggct ggaggaccgg gtgcagcagc tcgtcacctt
      301 cggcgaggac agccaggggg agatctacgt cgccgccctc aacggagcga ttcatcgagt
      361 caccggcgag gccgcgcccc cgccgccgcc cccgccgtcc tcggacaggg tcggggtcta
      421 cgagccggcc accgcccgct tccacctcaa ggaccggctc atcaccggcc cggccgaccg
      481 cgtggtgcgc ttcggcaggg gcaacagccc ttggcgcccc ctcgccggcg actggaatgg
      541 cgacggcacg gacaccatcg gcctgtacga cgccccccgg ggcctcttcc ggctcaagaa
      601 cacgctgacc ggcggcaacg ccgacgtgct gctgcggctc cgtctgcccg ccggccccaa
      661 ccagaacgcc atcccggtgg ccggcgactg ggacggcgac gggcgcgacg gcgtcgggct
      721 cttcggctcc gccaccacct tctatctgaa ggacaccgtc gacggcggcg acttcgacgc
      781 ggtctgcggg ttcgacctgc tgggccggcc gccggcccag tacctcccca tcgccggcga
      841 ctgggacggc gacgggaggg actcgatcgg gctctaccgg ccggcgaccg gcacgttcct
      901 cctcgccgac gggtgcgcgg accaggagcc ggagctggaa ttccagttcg ggtccggcgg
      961 ccgggcgctc cccctggccg gcgactggga cggcgacgga cgcgacggca tcggcctcta
     1021 cgatccgcgc aacggcgcct tcgcgctgaa gaacgagcct ggaggcggcg ccgcggacgt
     1081 cagcttccgg atccggccgc gcccccgcaa tggcgctcct ctggccggtg cctggtaggt
     1141 gaaggagtag aatcgggccc ctatacgtgg aggagatgcc atgaagggaa tgctcgccgg
     1201 tctcggggcc ctgggcctcg cgctgctcgc cgccgccccg gccacgccgg cgctggcggc
     1261 ggagacgtac gacctcatcc tgcgccgtgg gacgatctat gacggcggcg gcggcaagcc
     1321 gttcaccggc gacgtggccg tccgcggcga ccgagtcgcc gccgtgggcg acctggggga
     1381 tgccaaaggc aaggtggagg tggacgccgc cgggctcgcc gtctctccgg gcttcatcaa
     1441 catgctgagc tgggccaccg agtcgctgat cgtggacgga cgggggcaga gcgacatccg
     1501 gcagggggtc accctggagg tgatgggcga gggttggtcc atggggccgg tcaacgccgc
     1561 catgaagaag gaggccctgg aggagcaggg ggacgtcaag ttcgacatcc cctggaccac
     1621 cctgggtgag tacctggatc acctggtgaa gcgcggcatc gcccccaacg tggcctcctt
     1681 cgtcggcgcc acgacgctgc gggtgcacga gctgggctac gagaaccggc cgccgaaacc
     1741 ggaggagctg gcgcggatgc gcaagctggt agcccaggcc atggaggagg gggcgctggg
     1801 cgtggggtcg tcgctgatct acgctccggc cttctatgcc aagaccgacg agctggtggc
     1861 cctgtgcgag gaggccggca ggtacggggg gatgtacatc tcccacatgc ggagcgaggg
     1921 caaccggctg gtcgaggcgg tggacgagct gatcaccatc gcccgccggg ccaaggtgcc
     1981 ggcggagatc taccacctca aggcggcggg caagtccaac tggggcaagc tcgaccaggt
     2041 gatccgcaag gtcgaggagg cgcgggcctc gggtctgcgc atcaccgccg acatgtacaa
     2101 c
//
LOCUS       DASPJI010000667        11269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_610579, whole genome shotgun sequence.
ACCESSION   DASPJI010000667 DASPJI010000000
VERSION     DASPJI010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11269)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610579"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VEL74_18940"
     CDS             <1..488
                     /locus_tag="VEL74_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64664.1"
                     /translation="RVYQQVFATFPLVRRWEREEYRLGPELRLYRIPDPPDCGPPADL
                     PATAAFVPDGSMRPRPKRPIEYTVDGQWSAFKGCFDAGTYRVTVRGEPTGPGELEVAA
                     ISGGGGPRLGRGPLDAAAGREVRLPRRGKYLFYIYLPVGSRVRGVTVTASSPPARRSA
                     P"
     gene            complement(448..2241)
                     /locus_tag="VEL74_18945"
     CDS             complement(448..2241)
                     /locus_tag="VEL74_18945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HYG64665.1"
                     /translation="MITEPKTTYEVGQWDLTELVPQADEATLARHFAEIEDAVRALES
                     RRELLRPGMDPRELVDALRLYEGLVEAMNRLSGYSSLWFYADTASERALTFRNRVRQV
                     STQASNRSLFFTLWWRSLTDEEAQPLLPTAPEHADYRHYLEDLRRLKPYTLDEKSEQI
                     INIKDDNGISAVTTLYSMLTNRLEYTLVVDGEEKKLTRDALMSYAFSPRADLREAAYQ
                     ELYRVYAQEAPILGQIYVNRVRDWHDENVGLRGYRSAISVRNIDNDIPDRAVEVLLDV
                     ARQNAPLFQRYFRLKAGWLGLPRLRRYDLYAPLAASDRQISYGEAVRSVLDTFEDFHP
                     LFAEQAERVFAQSHVDSEIRKGKRGGAFCFTVLPSYTPWVLVNFAGKMRDVATLAHEL
                     GHAIHSMMAEKHSVLTQHASLPLAETASVFGEMLMTDRLLRDERDPLVRRELLAASVD
                     DVYATVLRQAYFVLFERDAHEAIMAGKSVEEITEMYVEKLREQFGDSVEVAPEFRYEW
                     LSIPHIYQTPFYCYAYSFGQLLVLALYRRFQEEGEAFKPGYLRLLAYGGSARPEAILR
                     ESGIDMTDPAFWQGGFDLVRERIDELEGMKL"
     gene            complement(2330..5668)
                     /locus_tag="VEL74_18950"
     CDS             complement(2330..5668)
                     /locus_tag="VEL74_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HYG64666.1"
                     /translation="MPPTAIRRLTRLPLLALLPVLLWGAASAPAAEKAAERNPLDPAR
                     LEAALQWREVGPYRGGRSAAAAGIPGQRDVYYFGATGGGVWKTLDGGRTWKNVSDGYF
                     GGSIGAVAVSEWDPNVVYVGGGEKTVRGNVSHGDGMWKSTDAGKTWKHVGLADSRHIS
                     RVRIHPKNPDLVYAAVLGHLFGPNEMRGVYRSADGGATWKRLLYVDESVGAVDLAMDP
                     TNPRVLYAGMWRVRRLPWGFESGGPGSGLWKSIDGGDSWTELTRNPGLPQGTVGIFGV
                     TVSPTNPENLYAIIEAQDGGVFRSQDGGKTWKRTNDKRDLRQRAWYYTRIYADPADEE
                     SVYVLNVRFHHSKDGGKTFTSIRTPHADNHDLWIDPADPARMIETNDGGANVSTDGGK
                     TWTPQDNQPTAQIYRVTTDNHFPYRILGAQQDNTALRIASRGAEGGIGAADFQETAGG
                     ESGHVVADPTDPEVVYGGSYGGFLTRLDHRTGALRDVNPWPDNPMGWGAAELEHRFQW
                     NFPLFFSPHDPKKLYAASQYLLESLDGGASWRRISPDLTRNDKSKLGPSGGPITKDNT
                     SVEYYATVFAAAESPLEPGVLWAGSDDGLVHVSRDGGKNWKNVTPRGLPEWAMINSLD
                     PHPFEKGGAYVAATRYKLDDFRPYLYRTTDYGATWTRIDAGIDPSHFTRVVRADPGRR
                     GLLYAGTERGVYVSRDDGGRWQPLQLNLPIVPVTDMTIKGDDLIAATQGRGFWVLDDL
                     TPLRQLTAEAAKRPVVLLAPEPVYRLSSAGNGEAPAGQGTNPPAGAVVGYWLRDAPAP
                     GTEVKLEFLDRDGKLIRSFATPAKAADPKAAQAKAGEPAPALPEAGEPETGLPGQADS
                     LAGEEKSQEEDAGDEDEDDKDPEVTARAGLNRFVWDLRYPRAEKFPGMVLWNNELQGP
                     MAVPGEYQVRLTVGAQEPQTARFEVRKDPRSAATQEDLEAQLQFLRSTRDKLTEAHDA
                     IRRIRDVRAQLDDLGKRLGAGSAEDSAEGSKEVLAAAKALDGKMTAVEQALYQTRNRS
                     SQDPLNFPIRLTDKLAGVAASAGTGDYRPTAQAVQVRDQLVAAIDQELAKLEAIWSQD
                     LPAFNQTASGAGVATVIVPAPRLQ"
     gene            complement(5787..6248)
                     /locus_tag="VEL74_18955"
     CDS             complement(5787..6248)
                     /locus_tag="VEL74_18955"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYG64667.1"
                     /translation="MKLGDVLKKERERKKLSFEDTASKLGLSEDEYREMEAGSSPAEK
                     WGPLLAKVAIKLETPTSRLLADSGKSADTKQGQAAELIRKHRERRGKTVEQMAEGLEI
                     SKEEYEEIENGQSGIEKYGPLLLHFAELIEQPVFNLFYPCGLPLEKLDDYP"
     gene            6519..7580
                     /gene="sbnA"
                     /locus_tag="VEL74_18960"
     CDS             6519..7580
                     /gene="sbnA"
                     /locus_tag="VEL74_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HYG64668.1"
                     /translation="MSQDLRKSGVLDGVRQGVLSAVGSTPIVRLERVYPDLEFRLYGK
                     LEALNPGGSIKDRPARAILEEALRTGEVNRETVIIESSSGNMGIGLAQACCYHGLRLI
                     CVVDPKSSRLNLQILQAYGAEIDFVPEPDPATGEFLQARLNRVQELRRIHPNSFWPNQ
                     YANANNSGSHYRTTMQEIVDTLGREVDYVFCATSTCGTVRGCGEFVRDHGLSTQVVAV
                     DAKGSLIFSSEQGKRLFPGMGAGLRPPLCDIDLIHQCVLVSDLECVVGCRRLVRREGI
                     LSGASCGGVIAAVEQMRDKIPAGSTAVLVLPDRGERYLDTVYSDEWVLEHFGDVFNMF
                     ESPFTPFEEKASSCAPAVC"
     gene            7559..8587
                     /gene="sbnB"
                     /locus_tag="VEL74_18965"
     CDS             7559..8587
                     /gene="sbnB"
                     /locus_tag="VEL74_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnB"
                     /protein_id="HYG64669.1"
                     /translation="MRPGSVLILRGDEIDALLTGHELEILEAVRQAYIIHGRGQSSLP
                     HSLFLRFPNDDLNRIIALPAYLGDGFDLAGMKWIASFPGNLQQGLARASAVLILNSAR
                     TGLPEAILESSIISARRTAASAALAARTFRQGQATPAAGFIGTGVINLETVRFLSRAV
                     PDIERFVLFDLDRERAAAFADKARAVLPEGSKAEMVIASDQREVLRSCPLVAFATTAV
                     KPYVSDLSDCAPGTTILHTSLRDFTPEVILAADNVVDDVDHAVRASTSVHLAEQQVGH
                     RAFLRGTLADILEGRAPAKANPDQLTIFSPFGLGVLDLAVGQMARSLALESGQGTVIE
                     SFLPTTGV"
     gene            8592..10199
                     /gene="serA"
                     /locus_tag="VEL74_18970"
     CDS             8592..10199
                     /gene="serA"
                     /locus_tag="VEL74_18970"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HYG64670.1"
                     /translation="MPRVLIADPLSEAGLALLREAGVEVVELGTGDRARLLDLVEDAD
                     ALLVRSRTKVDAELLRAGRRLKVVGRAGIGVDNVDVATATELGILVINAPTANLMSAT
                     EHTFALLLALARNVPAADASMKAGEWDRNRFVGTELQGKTLGIIGLGRIGQRVAARAK
                     AFEMNVVAFDPFLDPDQGPRLGVDLLLLDDLLRRADVVTLHTPYTEQTRDLLNAARIA
                     LMKPGALLVNCSRGGVVDEAALLKALESGHLGGAALDVFADEPPKDLSLAGHPKVVAT
                     PHLGAQTAEAQERTSTETAQMVLAALQGSYAVTAVNLPFRSSGGSRGEPYLRLGEQLG
                     RLSSALLRSPLKRVQVDLWGIDEGLQFPLTLAVLKGVLSPFLGTAVNYVNVERLAQSR
                     GVETVRAAHGEPGEYPALIGVTLHGSDAGADGRVELAGTLFGDRQPRVVRFGRQPLEF
                     RPEGKLLILRSFDVPGNVGKVGTLLGEAGVNIADIHLARKDGEEDAWTILRLDEEPPQ
                     SLLERLAALPQMRRVHLADLGRAGEPA"
     gene            10287..>11269
                     /gene="ftsY"
                     /locus_tag="VEL74_18975"
     CDS             10287..>11269
                     /gene="ftsY"
                     /locus_tag="VEL74_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HYG64671.1"
                     /translation="MADEKKNDILELAPETRLAEEEVQQGGFFKKLKRGLFMTHTEMI
                     EKISSAVAGKVALDESTLEYLEEVLISADLGVDTSLELVERVRKNALAEQVGDPKRLR
                     KMLADEMAGLLWELPKAKPLGRGPTITLVVGINGVGKTTSIAKLARRHQMAGERVLLA
                     AGDTFRAAAIEQLSLWGQRLGIEVIKQAQGSDPAAVIYDALQAAKARDIDQLFIDTAG
                     RLHTKDHLMAELSKIKRVIDREAAGWNRRTILVLDATTGQNALAQARTFCQVAQVDGV
                     LLAKLDGTAKGGMAVAVAKELRLPVLYLGVGEKAEDLVDFRPREFAAALLE"
BASE COUNT         1561 a         3945 c         3982 g         1781 t
ORIGIN      
        1 cccgcgtcta ccagcaggtc ttcgccacct tccccctggt gcggcgctgg gagcgcgagg
       61 agtaccggct gggccccgag ctgcggctct accgcatccc cgatcccccc gattgcggtc
      121 ctcccgccga tctacccgcg acggcggcct tcgtgccgga cggcagcatg cgcccgcggc
      181 cgaagcggcc catcgagtac acggtggacg gccagtggtc cgccttcaag gggtgcttcg
      241 acgccgggac ctaccgggtg acggtgcgcg gggagccgac ggggccgggg gagctggagg
      301 tcgccgcaat ttccggtggg ggtggcccaa ggctggggcg gggacccctg gatgccgccg
      361 cgggccggga ggtccggctg ccgcggcggg ggaaatatct cttctacatc tacctcccgg
      421 tggggagccg ggtgcggggg gtgacggtta cagcttcatc ccctccagct cgtcgatccg
      481 ctccctgacc aggtcgaagc cgccctgcca gaaggcgggg tcggtcatgt cgatgccgga
      541 ctcgcggagg atggcctcgg gccgcgccga gccgccgtag gccagcaggc gcaggtagcc
      601 gggcttgaag gcctccccct cctcctggaa gcgccggtag agcgccagca ccaggagctg
      661 gccgaagctg taggcgtagc aatagaacgg cgtctggtag atgtggggga tgctcagcca
      721 ctcgtagcgg aactccggag cgacctcgac gctgtcgccg aactgctccc ggagcttctc
      781 cacgtacatc tcggtgatct cctccaccga cttgccggcc atgatcgcct cgtgcgcgtc
      841 gcgctcgaag aggacgaagt aggcctggcg cagcaccgtg gcgtagacgt cgtccaccga
      901 cgccgccagc agctcgcggc ggacgagcgg gtcgcgctcg tcgcgcagca gccggtcggt
      961 catcagcatc tcgccgaaca ccgaggcggt ctcggccagg gggagcgagg cgtgctgggt
     1021 gagcaccgag tgcttctcgg ccatcatgct gtggatggcg tgcccgagct cgtgcgccag
     1081 cgtcgccacg tcccgcatct tgccggcgaa gttcaccagc acccaggggg tgtagctcgg
     1141 cagtacggtg aagcagaagg cgccgccgcg cttgcccttg cggatctcgc tgtcgacgtg
     1201 gctctgcgcg aacacccgct cggcctgctc ggcgaacagg gggtggaaat cctcgaaggt
     1261 gtcgagcacc gagcgcaccg cctcgccgta ggagatctgg cggtccgagg cggcgagcgg
     1321 cgcgtacagg tcgtagcggc gcagccgcgg gaggcccagc cagccggcct tgagccggaa
     1381 atagcgctgg aagagcggcg cattctgccg cgccacgtcg agcagcacct ccaccgcccg
     1441 gtccgggatg tcgttgtcga tgttgcgcac cgagatcgcc gaccggtagc cgcgcaggcc
     1501 gacgttctcg tcatgccagt cgcgcacccg gttgacgtag atctggccga ggatcggcgc
     1561 ctcctgggca tagacccggt acagctcctg gtaggccgcc tcccgcaggt ccgcccgggg
     1621 cgagaaggcg tagctcatca gcgcgtcgcg ggtgagcttc ttctcctcgc cgtccaccac
     1681 cagggtgtac tccagccggt tggtgagcat ggagtagagc gtcgtcaccg ccgagatgcc
     1741 gttgtcgtcc ttgatgttga tgatctgttc cgacttctcg tccagcgtgt agggcttgag
     1801 ccggcgcagg tcctccaggt agtggcggta gtcggcgtgc tcgggcgcgg tgggcagcag
     1861 cggctgcgcc tcctcgtcgg tcaggctccg ccaccagagg gtgaagaaaa ggctgcggtt
     1921 ggacgcctgc gtcgagacct gccgtacgcg gttgcggaag gtcagcgccc gctcgctggc
     1981 ggtgtcggcg tagaaccaga gggaggagta gccggagagc cggttcatcg cctccaccag
     2041 cccctcgtag aggcgcagcg cgtcgaccag ctcccgcggg tccatccccg gccgcagcag
     2101 ctcccgccgc gactccaggg cgcggaccgc gtcctcgatc tcggcgaaat gccgggccag
     2161 cgtggcctcg tcggcctgcg ggaccagctc cgtcaggtcc cactggccca cttcataggt
     2221 cgtcttcggt tcggtgatca cggtcatttt cgtcagttcc ccgggtcgct tgggttgtcg
     2281 gttcccgttg tcgattcctg caaacggggc aaggcctcgg cccagccggt tattgtagcc
     2341 gaggcgcggg tacgatcacc gtcgccaccc ccgcgccgct ggccgtttga ttgaacgccg
     2401 gcagatcctg gctccagatc gcctccagct tcgcgagctc ctggtcgatc gccgccacca
     2461 gctggtccct cacctggacc gcctgcgccg tgggacggta gtcgcccgtg cccgccgacg
     2521 ccgccacgcc ggccagcttg tcggtcagcc ggatcgggaa gttgagcggg tcctgcgagc
     2581 tccggttccg cgtctgatag agcgcctgct cgaccgccgt catcttgccg tccagcgcct
     2641 tggccgcggc gaggacctcc ttggagccct ccgcggagtc ttccgcggag ccggcgccca
     2701 ggcgcttgcc gaggtcgtcg agctgcgccc gcacgtcgcg gatgcggcgg atggcgtcgt
     2761 gggcctcggt gagcttgtcc cgcgtcgacc gcaggaactg gagctgcgcc tcgaggtctt
     2821 cctgcgtggc cgccgagcgc gggtccttgc gcacctcgaa gcgggcggtc tgaggctcct
     2881 gggcgccgac ggtgaggcgc acctggtact cgccggggac cgccatcggc ccctggagct
     2941 cgttgttcca caggaccatg ccggggaact tctcggccct ggggtagcgc aggtcccaga
     3001 cgaagcggtt gagccccgcc cgagccgtga cctcggggtc cttgtcgtcc tcgtcctcgt
     3061 cgcccgcgtc ctcctcctgg ctcttctcct cgccggccag cgaatccgcc tggccgggaa
     3121 gaccggtctc cggctccccc gcctccggca gcgcgggagc cggctcgccg gccttcgcct
     3181 gcgcggcctt ggggtccgcg gccttcgccg gggtggcgaa ggagcggatc agcttgccgt
     3241 cgcggtcgag gaactcgagc ttcacctcgg tgccgggagc gggggcgtcg cgcagccagt
     3301 agccgaccac ggcgccggcg ggcgggttcg tcccctggcc ggccggcgcc tcgccgttgc
     3361 cggcgctcga cagccggtag accggctccg gcgccagcag gaccaccggc cgcttggccg
     3421 cctcggcggt gagctggcgc agcggcgtca ggtcgtccag cacccagaag ccgcgcccct
     3481 gggtggctgc gatcaggtcg tcccccttga tcgtcatgtc ggtgaccggc acgatcggca
     3541 ggttgagctg cagcggctgc cagcggccgc cgtcgtcgcg cgacacgtag accccgcgtt
     3601 cggtgccggc gtagagcagg ccgcggcgcc ccgggtcggc gcgcacgacg cgggtgaagt
     3661 gggacggatc gatgccggcg tcgatgcgcg tccaggtggc gccgtagtcg gtggtgcggt
     3721 agaggtaggg ccggaagtcg tccagcttgt agcgggtggc cgccacgtag gcgcccccct
     3781 tctcgaacgg gtggggatcg aggctgttga tcatcgccca ctcgggtagg ccgcgcgggg
     3841 tgacgttctt ccaattcttc ccgccgtccc gcgacacgtg gaccaggccg tcgtcggagc
     3901 cggcccagag cacccccggc tcgagcggcg actcggcggc ggcgaagacc gtggcgtagt
     3961 actcgacgct ggtgttgtcc ttggtgatcg ggccgcccga cgggccgagc ttcgacttgt
     4021 cgttgcgcgt caggtcgggc gagatgcgcc gccaggaggc gccgccatcg aggctctcca
     4081 gcaggtactg gctggccgcg tacagcttct tcgggtcgtg cggcgagaag aagagcggga
     4141 agttccactg gaaccggtgc tcgagctcgg ccgcccccca gcccatgggg ttgtccggcc
     4201 acgggttgac gtcgcgcagc gccccggtgc ggtggtcgag gcgggtgagg aagccgccgt
     4261 aggagccgcc gtagaccacc tcgggatcgg tggggtcggc gaccacgtgg ccgctctcgc
     4321 cgccggccgt ctcctggaag tcggcggcgc cgatcccccc ctcggcgccg cggctggcga
     4381 tgcgcagcgc ggtgttgtcc tgctgcgcgc cgaggatgcg gtaggggaag tggttgtcgg
     4441 tcgtcacccg gtagatctgc gccgtgggct ggttgtcctg cggcgtccag gtcttgccgc
     4501 cgtcggtcga gacattggcg ccgccgtcgt tggtctcgat catccgcgcc gggtccgcgg
     4561 ggtcgatcca caggtcgtgg ttgtcggcgt gcggcgtgcg gatggaggtg aaggtcttgc
     4621 cgccgtcctt ggagtggtgg aagcggacgt tgagcacgta gaccgactcc tcgtcggccg
     4681 ggtcggcgta gatgcgggtg tagtaccagg cgcgctggcg caggtcgcgc ttgtcgttgg
     4741 tccgcttcca ggtcttgccg ccgtcctgcg aacggaagac gccgccgtcc tgggcttcga
     4801 tgatggcgta caggttctcc gggttggtgg gggagacggt gacgccgaag atgcccaccg
     4861 tcccctgggg gaggccgggg ttgcgggtca gctcggtcca ggagtcgccg ccgtcgatcg
     4921 acttccagag gccggagccg gggccgccgc tctcgaagcc ccagggcagg cgccgcaccc
     4981 gccacatgcc ggcgtagagg acccgcgggt tggtggggtc catggccagg tccaccgcgc
     5041 cgacgctctc gtcgacgtag aggagccgct tccaggtcgc gccgccgtcg gcggaccggt
     5101 agacgccgcg catctcgttg gggccgaaga ggtggccgag cacggcggcg tagacgaggt
     5161 cggggttctt ggggtggatg cggacgcgcg agatgtgccg cgagtcggcg agccccacgt
     5221 gcttccaggt cttgccggcg tcggtggact tccacatgcc gtcgccgtgc gagacgttgc
     5281 cgcgcacggt cttctcgccg ccgcccacgt agaccacgtt ggggtcccac tcggagacgg
     5341 cgacggcgcc gatggagccg ccgaagtagc cgtccgagac gtttttccag gtccgcccgc
     5401 cgtccagggt cttccacacg ccgccgccgg tggcgccgaa gtagtagacg tcgcgctggc
     5461 ccggaatccc ggcagcggcc gccgagcggc cgccgcggta ggggccgacc tcgcgccact
     5521 gcagggcggc ttcgaggcgc gccgggtcga gcgggttcct ctctgcagcc ttctcagcgg
     5581 cgggggccga agccgctccc cagagcagga cggggagcag ggcgaggagc gggagccgcg
     5641 tcaggcggcg gatggcggtg ggcggcatgg gatccctcct ggagattgat catcgtacgt
     5701 cacgaaatac gaacggcctg ccggagcagg ccgattcgca ccacctttcc cgcggcaccg
     5761 ccggagcggg gcgggagagc ccagacctac gggtagtcgt ccagcttctc gagcggcagg
     5821 ccgcaggggt agaagaggtt gaacaccggc tgctcgatga gctcggcgaa gtggaggagc
     5881 agcggtccat acttctcgat gccggactgg ccgttctcga tctcctcgta ctcctccttg
     5941 gagatctcca gcccttcggc catctgctcg accgtcttgc cgcgccgctc gcggtgcttg
     6001 cggatcagct cggcggcctg cccctgcttg gtgtcggccg acttgccgct gtcggccagc
     6061 aggcgcgacg tcggggtctc cagcttgatg gccaccttgg ccagcagcgg gccccacttc
     6121 tcggcgggag agctgcccgc ctccatctca cggtactcgt cttcggacaa cccgagcttg
     6181 ctcgcggtgt cttcgaaaga aagcttcttg cgctctcgtt cttttttcag tacgtctccg
     6241 agtttcacgg tcccctcctt gtaacgttga aacggcccac gagcttcgtc gctccccggg
     6301 gcacgagaga acccgcccgg ggccgattgc ctcatcgatt gggcgccggg atgctagcac
     6361 agggggggct gtttgctcta accaaacgag agccgggctg ctttgtcccg ctttcacccc
     6421 ctggtgatac ctttatcgga gggtgagctg tccattcgcg gatcgccctg aatttttcag
     6481 atattcataa ggcccacgat ctcaaggagc tctgtatctt gagccaggat ttacggaagt
     6541 caggtgttct cgatggcgtg cggcaggggg tgctctcggc cgtggggagt acgcccatcg
     6601 ttcggttgga gagggtgtat ccggacctcg agttccggct ctatggcaag ctggaggcgc
     6661 tgaacccggg cggcagcatc aaggaccggc cggcgcgcgc catcctggag gaggcgctgc
     6721 gcaccggcga ggtgaaccgg gagacggtca tcatcgagtc gagctcgggc aacatgggga
     6781 tcgggctggc ccaggcctgc tgctaccacg gcctgcgcct catctgtgtg gtcgatccca
     6841 agtcgtcgcg gctcaacctg cagatcctgc aggcctatgg cgccgagatc gacttcgtcc
     6901 ccgagccgga tccggccacc ggcgagttcc tgcaggcgcg cctcaaccgc gtccaggagc
     6961 tgcggcggat ccacccgaac tcgttctggc ccaaccagta cgccaacgcc aacaactcgg
     7021 ggtcgcacta ccgcaccacc atgcaggaga tcgtcgacac cctgggccgc gaggtggact
     7081 acgtgttctg cgccaccagc acctgcggca ccgtgcgcgg ctgcggcgag ttcgtgcgcg
     7141 accacggcct gtccacccag gtggtggcgg tggacgccaa ggggagcctg atcttctcca
     7201 gtgagcaggg caagcgcctc ttcccgggca tgggcgccgg cctgcggccg ccgctgtgcg
     7261 acatcgacct catccaccag tgcgtgctgg tgagcgacct ggagtgcgtg gtcgggtgcc
     7321 ggcgcctggt gcggcgcgag ggcatcctct ccggcgcctc ctgcggcggc gtgatcgccg
     7381 ccgtggagca gatgcgcgac aagatcccgg cgggctccac cgccgtcctc gtcctgccgg
     7441 accgcggcga gcgctacctc gacaccgtct actccgacga gtgggtgctc gagcacttcg
     7501 gcgacgtctt caacatgttc gagagcccct tcacgccgtt cgaggagaaa gcgtcgtcat
     7561 gcgccccggc agtgtgctga tcctgcgcgg cgacgagatc gacgccctgc tcaccgggca
     7621 cgagctcgag atcctcgagg ccgtgcgtca ggcctacatc atccatggcc gggggcagag
     7681 ctcgctgccg cactcgctct tcctgcgctt cccgaacgac gacctcaacc gcatcatcgc
     7741 cctgcccgcc tatctcggcg acggcttcga cctggcgggc atgaagtgga tcgcctcgtt
     7801 ccccggcaac ctccagcagg ggctcgcccg cgcctcggcg gtcctcatcc tcaacagcgc
     7861 caggaccggc ctgccggagg cgatcctcga gtcgtcgatc atcagcgccc ggcgcaccgc
     7921 cgccagcgcc gccctcgccg cccggacgtt ccggcagggg caggcgacgc ccgccgccgg
     7981 cttcatcggc accggcgtca tcaacctgga gacggtccgc ttcctctccc gcgccgtgcc
     8041 ggacatcgag cgcttcgtcc tcttcgacct cgaccgcgag cgggcggccg ccttcgccga
     8101 caaggcgcgg gcggtgctcc cggagggctc gaaggccgag atggtcatcg cctccgacca
     8161 gcgcgaggtg ctgcggagct gcccgctggt ggcgttcgcc accaccgcgg tcaagcccta
     8221 cgtctcggac ctctccgact gcgcgccggg cacgaccatc ctccatacct cgctgcgcga
     8281 cttcaccccc gaggtcatcc tcgccgccga caacgtggtg gacgacgtgg accacgcggt
     8341 gcgggcgtcg acgtcggtgc acctcgccga gcagcaggtg gggcaccgcg ccttcctccg
     8401 cggcacgctg gccgacatcc tggagggccg ggcgccggcc aaggcgaacc cggaccagct
     8461 caccatcttc agccccttcg gcctcggcgt cctcgacctg gcggtcgggc agatggcgcg
     8521 gtcgctggcc cttgagtccg gccagggcac tgtgatcgaa tcgttcctgc ccaccaccgg
     8581 agtctgaaac gatgccaaga gtcctgatcg ccgaccccct ctcggaggcc ggtctcgccc
     8641 tgttgcgcga ggccggcgtg gaagtcgtgg agctggggac gggcgaccgc gcgcgcctcc
     8701 tcgatctggt ggaggatgcc gacgccctgc tggtgcgcag ccgcaccaag gtggacgccg
     8761 agctgctgcg cgccggccgc cgcctcaagg tggtgggccg cgccggcatc ggcgtcgaca
     8821 acgtcgacgt cgccaccgcc accgagctgg gcatcctggt gatcaacgcc cccacggcca
     8881 acctgatgtc ggccacggag cacaccttcg cgctgctcct cgccctcgcc cgcaacgtgc
     8941 cggcggcgga cgcctcgatg aaggcggggg agtgggaccg caaccgcttc gtcggcaccg
     9001 agctccaggg gaagaccctg ggcatcatcg gcctggggcg gatcggccaa cgggtggcgg
     9061 cgcgcgccaa ggcgttcgag atgaacgtcg tggccttcga cccgttcctc gaccccgacc
     9121 aggggccgcg gctcggcgtc gacctgctcc tcctggacga cctgctgcgc cgcgccgacg
     9181 tggtgacgct gcacacgccc tacaccgagc agacccggga cctgctgaac gcggcgcgca
     9241 tcgccctgat gaagccgggg gcgctgctgg tcaactgctc gcgcggcggc gtggtggacg
     9301 aggcggcgct actcaaggcg ctggagtcgg ggcacctggg cggcgcggcg ctggacgtct
     9361 tcgccgacga gccgcccaag gatctgagcc tggctgggca tcccaaggtg gtggcgaccc
     9421 ctcacctggg cgcccagacc gccgaagccc aggagcggac ctccaccgag accgcccaga
     9481 tggtgctcgc ggcgctgcag ggctcctacg cggtgacggc ggtcaacctg ccgttccgca
     9541 gcagcggcgg cagccgtggc gagccgtacc tgcgcctcgg cgagcagctc gggcgcctca
     9601 gctcggccct cctgcgcagc cccttgaagc gggtgcaggt ggacctctgg gggatcgacg
     9661 aggggctgca gttcccgctg acgctggcgg tgctcaaggg ggtgctctcg cccttcctcg
     9721 gcaccgcggt gaactacgtc aacgtcgagc gcctcgccca gagccggggc gtcgagacgg
     9781 tgcgggcggc gcacggcgag cccggcgagt acccggcgct gatcggcgtc accctccacg
     9841 gctcagatgc cggcgccgac gggcgggtgg agctggccgg gacgctcttc ggcgaccgcc
     9901 agccccgggt ggtgcgcttc ggccgccagc ccctggagtt ccgtcccgag ggcaagctgc
     9961 tgatcctgcg cagcttcgac gtccccggca acgtcggcaa ggtggggacg ctgctgggcg
    10021 aggcgggggt caacatcgcc gacatccacc tcgcccgcaa agacggcgag gaggacgcct
    10081 ggaccatcct ccgcctggac gaggagccgc cgcagagcct cctcgagcgc ctcgccgccc
    10141 tgccgcagat gcggcgcgtc cacctggccg acctcggccg ggcgggcgag ccggcgtagg
    10201 ccctcacccc cccggcccct ctcccgcctc cccatccccc accccgggag agggggaggc
    10261 tcatttcccg ctatcattgc cccgttatgg ctgacgagaa gaagaacgac atcctcgagc
    10321 tcgctcccga gacccgtctg gcggaagagg aggtccagca gggcggcttc ttcaagaagc
    10381 tgaagcgcgg cctcttcatg acccacacgg agatgatcga gaagatctcc tccgccgtgg
    10441 cgggcaaggt cgccctggac gaatccacgc tcgagtacct ggaagaggtc ctgatcagcg
    10501 ccgacctcgg cgtggacacc tccctcgagc tggtcgagcg cgtccgcaag aacgccctcg
    10561 ccgagcaggt gggcgatccc aagcgcctgc gcaagatgct ggccgacgag atggccggcc
    10621 tgctctggga gctgccgaag gcgaagccgc tgggccgcgg ccccaccatc accctggtgg
    10681 tggggatcaa cggcgtcggc aagaccacct ccatcgccaa gctcgcccgg cgccaccaga
    10741 tggccggcga gcgggtgctc ctggcggcgg gcgacacctt ccgcgccgcg gccatcgagc
    10801 agctctccct ctgggggcag cgcctgggga tcgaggtcat caagcaggcc cagggctcgg
    10861 acccggcggc ggtgatctac gacgccctcc aggcggcgaa ggcgcgcgac atcgatcagc
    10921 tcttcatcga caccgccggg cgcctccaca ccaaggacca cctgatggcc gagctgagca
    10981 agatcaagcg ggtcatcgac cgcgaggccg ccggctggaa ccggcggacc atcctggtgc
    11041 tcgacgccac caccggccag aacgccctcg cccaggcccg caccttctgc caggtcgccc
    11101 aggtggacgg cgtgctgctc gccaagctgg acggcacggc caaggggggc atggcggtgg
    11161 cggtggccaa ggagctgcgg ctgccggtgc tctacctcgg agtgggggag aaggccgagg
    11221 acctggtgga tttccggccc cgggagttcg ctgctgctct cctcgagtg
//
LOCUS       DASPJI010000668        18117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_675246, whole genome shotgun sequence.
ACCESSION   DASPJI010000668 DASPJI010000000
VERSION     DASPJI010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 18117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18117)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18117
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675246"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3221
                     /locus_tag="VEL74_18980"
     CDS             <1..3221
                     /locus_tag="VEL74_18980"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64672.1"
                     /translation="GYRSAPLVAAILGVFKAGAAYTILDPDYPAGRLVDYVNLVKPQG
                     WVRIPAAGLPQVELEEALSALPLRCRLDLEMSEEDCAAPDPLAALPSDEPGVDAGVLV
                     GPDDIAYISFTSGSTGMPKGILGRHGPLTHFLPWQTDTFGFFEDDRYSMLAGLSHDPL
                     QREIFTPLQLGATVCIPDPAEIGTPGYLARWMARERITIAHLTPAMGQLLAQGNQGDV
                     LQSEPPVLIPTLRYAFFVGDVLTRRDIERLRRLAPAITCVNYYGSTETQRAVGYHVIE
                     EGGGGDDERLGRQIVPLGRGMQDVQLLILGSAGELAGVGELGEIAVRSPHLARGYLGD
                     EDLSRSKFVVNPFTGEAGDRLYRTGDLGRYRPDGLAVFSGRADLQVKLRGFRIELGEI
                     EALLGRFPGVREAAVILREDRPGEKLLAAYVVAEDGRELPLDEVRRLLQSRLPAYMVP
                     AALLQLPELPLTPNRKLDRRALPAPLWSGGGEVAEDEPLTPLEELVAGVWREVLGVPR
                     ARRSDDFFALGGHSLLATQVLSRLGHALGLTLPIRAIFEHPTVAAMAGHLERQLRERS
                     STGAPPVVRVSRDQPLRLSFAQQRLFFLYRLEPDSAAFNLPTALRLTGELDVAALARS
                     FTGLVARHESLRTRFRQDGGEPLQVIDPPSPVPLPLADLSGLPVYVAGIEARRLAFQE
                     AQRPFDLDRGPVLRVLLVRLGSLDHTLAVTMHHIVSDGWSRGILVREMTRLYQLFSQE
                     RDPELPPLPFQYADIAAWQRDWLQGDELESQVAYWKERLADAPEVLALPLDRPRQAGS
                     PMPCGVESRGLPAALGEQLEALSRRSGVTLFMTLLTGFTVLLHRYCGQDDVVVGTDVA
                     NRNRIESEGVIGFFINQLVLRTDLSGDPKFMELLDRVRETTLGAYLHQDLPFDKLVEV
                     LKPGRSLGHSPLFQVKCTLQNVPRSTLSLPGLGIEAVEAHRGKAQIDLLLNLFEMSGG
                     VRASMEYNADLFDAPTIAKMLSAYELVLSTAASRPEARVSELLEVLAAADRSRRVEKA
                     KSFDDTFRSQLRGAGRRRSGQAQPPTAIGEADMDLREEKRST"
     gene            3218..17407
                     /locus_tag="VEL74_18985"
     CDS             3218..17407
                     /locus_tag="VEL74_18985"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64673.1"
                     /translation="MSEAIIGGFQLSPLQRRVWSLQERDSGQPYRALCLLRIDGDLSR
                     ERVKAAVRTVVARHEILRTGFERLPGMQLPLQVIRDGAPLELEPPDLRGLSAEERQAG
                     LAGLWEAAQRMPLDTDGGSPLVWSLVPLGSREHALLLAAPALCLDTAGLRALAAEVVG
                     ACGDGSPLDDDEVQEPVQYADYAEVLDNLLASEEAREGLEYWRRLDLSSLNDASLPFL
                     RRPAGAERIEPASMRVRLPRGVAEGADALARRLGVEPRVVYMAAWSLVLGRLTGRLAI
                     TLGVAYDGRRYQELAGAPGLFARHLPVQCGLEPSRTVSAELERIAEATAEHRDWQDCF
                     DWTALGLAGDGTAPPFFPFCFELEERAPLAEAGGLRVERLRQSVWADRFQLKLTCVRE
                     GAGMTAELQFDAAVLSAAAVGRLAAQLARLLESAIENPGQPIGALDLLASWTAHELLV
                     EDNDTALELGADRCLHELVEEQVAKTPSHTAVIFEGQDLTYEELNVRANRLAHALQRI
                     GVGPDVRVAVCMERAPEMVVSLLGILKAGGAYVPLDPGYPADRLELMLGDSGAALLLT
                     QERLLPVLPESGLPRICVDGEAELHWEEGAENPRVPVEPRNLAYVIYTSGSTGRPKGV
                     MIPHAAINNRLLWMGRRFGLGEGERVLQKTSFSFDASVWELFLPLLTGGIVVLARPGG
                     QQDNAYMARTIAREGVSVLQLVPSMLRLFLEEPEVNACGSLRHLFCGGEALSRDLQER
                     CFERLDAELVNLYGPTEVSIDATYWVCGRGSDLPLVPIGSPLSNIRVLLLDAEMRPVP
                     FGATGEVCVGGAGLARGYWGLPGTTAEKFVPDPFAPEPGGRLYRTGDLSRFVAPGVVA
                     FAGRVDHQVKVRGFRIEPGEIEAVLRSHEAVRDAVVVARKDAAGDHLVAYVVPRETDS
                     LSDLRRWLGERLPEHMVPAFVVELASFPLLPNGKVDRAALPAPEPERRSVEAYLAPRT
                     STEEVLATLWEDLLGCKRVGVHDDFFDLGGHSLLATQLVSRLRQGMGIELSLRAIFET
                     PTLAGLATEVDHVRRGGETSEAPPIRPVPRDRSLPLSFAQQRLWLLDRLAPGSAAYNL
                     PMGLRLEGPLQASALTRALSEIVRRHEALRTTFAELDGEPVQLIAAPAPWHLPVIDLD
                     ALPVERREGVSQELTRAQARRGFDLVRGPLLRVLLLRLGPGEHVLLATLHHIVSDGWS
                     TGILVRELAHLYERAVERQPTRLMELPVQYADFAVWQRGWLQGEVLEAHLDYWRHQLG
                     AEIPVLQLPTDRSRPAVQTTRGAVEAEWLSPRLTNELERLSRGEGVTLFMTLLAAFQA
                     LLARYSGQSDIVVGSPVANRHRLEIESVIGCFINTLVLRTDLSALGSFRELLGQVREV
                     ALGASAFQGLPFEKLVEELQPRRDLSRSPLFDVLFMLQNVPFEQLQLPGLTLSPMRVD
                     AGRAPFDWTLSLAPTERGLLASLEYNRDLFDAATAARALRHYERLLTTWTQTPGADVW
                     ETPLLSPAEEHQVRTEWNRWGDGFPTGVGVHQLIADQARRTPEGVAVVFEGEVWTYRD
                     LADRSAAVARRLRALGAGPGALVGLFVDRSAGMVAALLGTLEAGAAYVPLDPAQPRER
                     LALMLEEATPAVIVSQDHLRGDLPPVGAPVLFLEEIGEGEDSGGVPVPAFQPTGDEPA
                     YVLFTSGSTGRPKGVQVPHRALVNFLCSMRECPGIHSGDVWLAVTPISFDIAGLELYL
                     PLLAGARVVLASRETVVDGARLLSLLRESGATVMQATPAGWRLLLEAGWGSPDLTVLC
                     GGEALPRQLAEDLHARAREVWNLYGPTETTIWSTVERVKPGAGTAAVAIGLPIANTQV
                     HVLDRALRPVPAGLAGELCIGGVGLATGYLRRPDLTAERFIPDPCGSDAGARLYRTGD
                     LARRLADGRLEYLGRLDHQIKVRGFRIELEEIEAVLARHPEIRQTVVLARQDTPGEPR
                     IVAYLVGDGVVNDAGLRTFLKDRLPDYMVPSAFVRLAALPLTPNGKIDRRALPPPPAE
                     RGGDSALPATPVAEVLAGLWTELLAVDPIGLHDDFFALGGHSLMAARLIARVRETFGV
                     ELSLRRVFEAPTLAALAAAVDAALQEGRGAVAPPLRRMPSLAREGGAPLSFAQQRLWF
                     IDQLQPGSPAYNLPVVLRLSGDLDVEALRETLAEILRRHEILRTVYPMAEDGGPRVTV
                     SSEVGPALSVLDLRPVPAGEREGEALRLARAEVRRPFDLAAGPLMRTLLLRLGEADHV
                     AVFTIHHIVSDGWSMGVLVREVGQIYPALLAGLPSPLPDLPLQYADFAVWQREWLQGE
                     VLAERTAWWREQLAGIPAVLELPADRPRPAVQSHRGSTLPFALSAGATRDLRSLGRSR
                     RATLFMTLLGGFQTLLYRYTGQSDVVVGTPVANRDRSELEGLIGFLVNTLVLRARFEG
                     VPTFGDLLGRLRETALEAHARQELPFEKLVEELQPQRDLSYSPIFQAMLALQNAPAEA
                     LSLPGLEAEPLDARGNSAKFDLLLTLTESEAGLSGSMEYATDLFEAATIERLLGHLRV
                     LLEGAAADPGLHVSDLPLLTGAEERQVVREWNDTAFAIPDEFCLHQRIEAQAGLTPDA
                     TAVVWRDLSLTYRELNARANRLACHLRAAGAGPDGRVGVCLERSEALVIALLAVLKSG
                     AAYVPLDPGYPDERLTFMLQDARPSVCITDEAIRPRLTGAVPLLCLDTQREEVARWSG
                     KDLAASATLDNLAYVIYTSGSTGRPKGAMIPHRGICNRLVWMQAQYGLTGGDRVLQKT
                     PFSFDVSVWEFFWPLLAGACIVVAEPGGHRDSAYLVRTIGEQEVTILHFVPSMLQAFL
                     EEEHLEACDGVRLVVCSGEALPLDLQKRFFEVMGAELHNLYGPTEASVDVTYHACRRG
                     TPLRSVPIGRPIFNTQIYIVDPGLRPLPVGVAGELLIGGDGVGRGYLDRAALTAETFI
                     ADPFSAAPGARLYRTHDLARLLPDGAIEFLGRIDNQVKVRGFRIELGEIEAVLAEHPA
                     VREAAVMAQQGRSGAPSLVAWFVVQDEPLSVDGLRAFLRQRLPEYMVPTTFVRLPALP
                     LNANGKLDRKVLPSLAGERVEPGRTYAAPSTYLEKTLAEIWVQVLKVEEMGVHDNFFS
                     LGGDSILSLQVTAQARRRGLRLALQQIFMHQTIAELARALEETPAETGAVTVTDSGDE
                     PFGLLSPEDRRKLPDFVEDAYPLTMLQAGMLYHMGLNPDAPAYHNVNSVHLRGRFEAA
                     TFQEAVLQVVARHAALRTSFDLAGYSQGLQLVHRAAFLPVAVEDLRYLTATEQERAIE
                     ELMERETRRPFDLAQPPLLRLHVFLRSDDTFQLTLTEHHAIFDGWSLHSTLNEIFDRY
                     FTLLNGESVPEAAAPAPGAAFRDYLRLESDAAASASSRRFWDEKLSDCTVMELPSWHA
                     TARGSRPARVEMLPVPVPREISDALQQLSRSTGVPLKSVLLAVHLKVMSVLWGQEDVL
                     TGMGTHGRPEEAGGEDVRGLFLNMVPCRLRVRSGQTWRELARNAFELERELIPHQRYP
                     LAALQKSRGGQRLFDTLFNFVHFHVVSDMLQSGRLEVLGFRKYEATSFRLLTLFAISL
                     VKSQVTLELDFDGAELTREQVSTIGDLYARAMAAMAEAPDQSHDVLLPLSEAARHQAL
                     VEWPDTATAYPREAAVPELFELQAAQTPGAIAVEFGADRLTYDELDHQANQLACSLRR
                     LEVGPEVPVAVFMERSHRMVVTFLGILKAGGYYVPLDPSYPRERLEVMLDDSGARVLL
                     TEERLASLLPAPGLTVLRLDSQWEEVARESGAPLDRRSSADNLAYVIYTSGSTGRPKG
                     VSIPHRGIVRLVKGTNYVQVGADDHVAQASNTSFDAATFEIWGALLNGARVVGIPREV
                     ALSPQDLAATIRDRRITVLFQTTALFNQTVRLVPDAYAGIRRHLVGGEAFDPRAFEAL
                     QLGGRPPERFANIYGPTESTTYATWYPMDRLPDGTSVPIGRPIANTTTYVLSEAGQPV
                     PMATPGELHIGGDGLARGYLGRPELTAEKFVPHPFAATPGERLYRTGDLVRPLSGGDI
                     EFLGRRDLQVKIRGFRIELDEIRIVLEQHPAVVESFVMVREDQPGERRLVAYFVPVSG
                     GASEIQALREHLREKLPDYMVPSAFVPLDALPMSVNGKVDRRLLPTPDWSQPEKQESY
                     AGPRDAVEEALVEIWQQVLGVARIGIHDNFFELGGDSILSIQIVARAGEKGLRLTPGN
                     IFQHPTVAELAMIAEAAETATRASASASGTGEVPLTPIQTWFFEQDLPRPHHFNQSML
                     LEVLAPVPPAALATVVEHLVEQHDALRLRFERTASGWRQVQAPSGGCTSLSRVDLAAL
                     PAELWKAEVEAVCAQVQQSLDLTRGPLLRAVLIDRGAAGSARLMMAVHHLVVDAVSWR
                     VLLQDLQVAYRQVAAGGVALVPARTTSFQHWAERLREYAATAAIRQELSYWTDPARAE
                     AAFVPRDFLEGDNVKESIRAVAVWLEPDETQQLVQEVPRVYQTQINDALLTGLALAFA
                     RWTGERSLLVDLESHGREEIFPDVDLSRTVGWFTNLAPVLLRVEEGLEPEGSLKAVKE
                     QLRAVPRRGIGYGLLRYMAPEPGVAGELRRLPRAQVGFNYLGQVDQVLAESPLLRPAP
                     ESSGRPESRSGLRPHLLEITAVIVEGRLRVRLEYSKNLHERSTIDTLGRYFMEALRSI
                     VAHCLTAGTVGYTPSDFPRARLKQKDLDQFLTKLGGSRKGEAVEV"
     gene            17397..>18117
                     /locus_tag="VEL74_18990"
     CDS             17397..>18117
                     /locus_tag="VEL74_18990"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG64674.1"
                     /translation="MKSEEIDDIYELSPMQAGMLFHSLAAERSGVYVLQFVCPLTGGL
                     DETAFERAWEGLAARHAVLRTSFHWQELDKPLQVVHQKVALPLARLDWRSIPEEAQEQ
                     RLRSLLREDRVQGFDLSQVPLVRLLLIRTGDTAYRLLWTTHHILLDGWCLGLVLQELL
                     ELYDGFRRGEPVRLPPVRPYADYIEWLQSQDAAAAEAFWRRILSGFGAATRLGVDRLA
                     APAASDGAALDPGGEAVFAEES"
BASE COUNT         2525 a         6235 c         6314 g         3043 t
ORIGIN      
        1 acggctaccg gagcgcgccg ctggtggccg cgatactggg tgtattcaag gccggtgccg
       61 cgtacaccat cctcgatccc gactacccgg cgggccggct ggtggactac gtgaacctgg
      121 tgaagcctca gggttgggtt cggatccctg cggcgggcct gccgcaggtg gagctggagg
      181 aggccctgtc tgccctgcct ctccgctgcc ggctcgatct cgagatgagc gaggaggact
      241 gcgccgctcc ggacccactg gcggcgctgc cgtcggacga gcccggtgtc gacgccggcg
      301 ttctggtcgg cccggacgac atagcctata tctcgttcac ctccggctcg accggaatgc
      361 ccaaggggat cctcggccgc catggtccgc tgacccactt cctgccctgg cagacggata
      421 cgttcgggtt cttcgaggac gaccggtaca gcatgctggc gggcctctcc catgacccgc
      481 tgcagcggga gatcttcacc ccgctacagc tcggggccac ggtctgcatc cccgatcccg
      541 ccgagatcgg tacgcccggc tacctggcgc gctggatggc gcgggagcgg atcaccatcg
      601 cccatctgac gccggccatg gggcagctcc tggcccaggg gaatcagggg gacgtcctcc
      661 agagcgagcc acctgtcctc attccgaccc tgcgctacgc cttcttcgtc ggcgacgtgc
      721 tgacccggcg tgacatcgag cggctccgcc gcttggctcc ggcgatcacc tgcgtcaact
      781 actacggctc gacggagacc cagcgggccg tggggtacca cgtcatcgag gagggcggtg
      841 gaggagatga cgagaggctc ggccgccaga tcgtaccgct cggccgcggc atgcaggacg
      901 tccagctcct gatcctcggc tccgccggtg agctggccgg cgtcggcgag ttgggggaga
      961 ttgccgtgcg cagcccccac ctcgcccgcg gctacctggg agacgaggac ctgagccgat
     1021 cgaagttcgt agtcaatccc tttaccggcg aggcagggga ccggctgtac cgcaccggcg
     1081 atctcggccg ctaccgtccc gacggcctcg ctgtgttctc cggccgcgcc gacctgcagg
     1141 tcaagctgcg cggcttccgc atcgagctgg gcgagatcga agcgctgctg ggccgattcc
     1201 ccggggtacg cgaagccgcc gtcatcctgc gcgaggatcg gcccggggag aagctcctgg
     1261 cggcctatgt ggtggccgag gacggccgcg agctcccgct cgacgaggtg cgccggctct
     1321 tgcagtcgcg cctgcccgcc tacatggtgc cggccgccct cctgcagctt cccgagctgc
     1381 cgctcacgcc gaaccgtaag ctggaccggc gcgctcttcc ggcgccgctg tggagcggcg
     1441 gcggagaggt ggcggaggac gagccgctca cgccgctgga ggagctggtc gccggcgtct
     1501 ggcgcgaggt cctgggagtg ccgagagcgc ggcggagcga cgatttcttc gcgttgggag
     1561 ggcactccct gctggccacc caggtcctgt cgcggctcgg ccacgcgctg ggcctgacac
     1621 tgccgatccg ggcgatcttc gaacatccca cggtcgccgc catggccggt cacctggagc
     1681 ggcagctgcg cgagcgcagt agcacgggtg cgccgccggt ggtgcgggta tcccgcgacc
     1741 agcccctgcg gctgtcgttc gcccagcagc ggctgttctt cctctatcgt ctggagccgg
     1801 acagcgccgc cttcaacctg ccgacggccc tgcggctcac cggcgagttg gacgtcgccg
     1861 ccctcgcccg gagcttcacc gggctcgtgg cccggcacga gtcgctccgc acccgcttcc
     1921 ggcaggacgg cggtgagccg ctgcaggtca tcgatccccc cagcccggta ccgttgccgc
     1981 tggcggacct gagcggcctg ccggtgtatg tggcgggtat cgaggcgcgg cggttggcgt
     2041 ttcaggaggc gcagcggccc ttcgacctcg accgaggccc ggtcctgcgt gtcctgctgg
     2101 tccgcctggg gagcctcgac cacacgctcg ccgtcaccat gcaccacatc gtcagtgacg
     2161 gctggtcgcg cggcatcctg gtgcgggaaa tgaccaggct ctatcagctg ttctcccagg
     2221 agcgcgaccc ggagttgcca cccctgccct tccagtacgc cgacatcgct gcctggcagc
     2281 gcgactggct ccagggagac gagctcgaga gtcaggtggc ctattggaag gagcggttgg
     2341 ccgacgctcc ggaggtgctc gccctgccgc tcgaccgccc ccgccaggcc ggctccccga
     2401 tgccgtgcgg cgtcgagagc cggggtctcc cggcggcgct cggcgagcag ctcgaggcgc
     2461 tgagccggcg ctccggtgtc acgcttttca tgactttgct cactggattc acagtcctcc
     2521 ttcatcggta ctgtggtcag gacgatgtgg tcgtaggaac ggatgtcgcg aatcgaaacc
     2581 ggatcgagag cgaaggagtc atcgggtttt tcatcaatca gctggtcctg cgcaccgatc
     2641 tgtcgggaga tcccaagttc atggagctcc tcgatcgggt gcgggagact actctcggag
     2701 cgtatctgca ccaggatctg ccgttcgaca agctcgtgga ggtcttgaag cctggacgaa
     2761 gcctcggcca ttcgcctctg ttccaggtga agtgcacctt gcagaacgtc ccacgatcca
     2821 cgctgagcct gccggggttg ggaatcgagg ccgtcgaggc ccatcgcggc aaggcacaga
     2881 tcgacctttt gttgaacctg tttgaaatgt ctgggggcgt tcgcgcatcc atggagtaca
     2941 acgccgatct cttcgacgca ccgactatcg cgaagatgct gagcgcctac gagctcgtgc
     3001 tgagcaccgc cgcctccagg ccggaagccc gggtcagtga gctgctggag gttctcgccg
     3061 cagcggacag gagccgccgg gtcgagaagg cgaagagctt cgacgacacc ttccgcagcc
     3121 agctcagggg ggcgggccgg aggcggtctg gacaggcgca gcctcccacg gcgatcggcg
     3181 aagcggatat ggatctgcgt gaggagaaga ggagcacatg agcgaagcga tcatcggagg
     3241 ttttcagttg tcgccgctgc agcggcgggt ctggtcgctg caggagcgtg actctgggca
     3301 gccttatcga gcgctctgcc tcctgcggat cgacggcgac ctgtcgcggg agcgcgtcaa
     3361 agcggcggtg aggacggtcg tggcgcggca cgagatcctc cgtaccggat tcgagcggtt
     3421 gccgggcatg cagctcccgt tgcaggtgat ccgggatggc gcaccgttgg agctggagcc
     3481 gccggacctg cggggccttt ccgcagagga gcggcaggcc ggcctggccg gcctgtggga
     3541 ggcggcccag cggatgcccc tggatacgga cggtggctct cccttggtct ggtccctggt
     3601 ccccctcggc tcccgagagc acgccctgct cctggcggct ccggccctct gcctagacac
     3661 cgcgggcctg cgcgctctgg cagcagaggt tgttggagcc tgcggggacg gctctccgct
     3721 cgatgacgat gaggtgcagg agccggtcca atacgccgac tatgccgaag tcctggacaa
     3781 cctcttggcc tctgaggagg cgcgggaagg gctcgagtac tggcgccggc tcgacctgtc
     3841 gagcctgaac gatgcgagcc ttcccttcct gcgccggccg gccggcgcag agcggatcga
     3901 gccggcgtcg atgcgcgtcc gcctgccgcg cggcgtggcg gaaggggccg atgccctggc
     3961 tcggcggctc ggcgtcgagc ctcgcgtggt ctacatggcg gcctggagtc tggtgctcgg
     4021 acgcctgacc ggccgtctgg cgatcacgct gggggtggcc tacgatggcc gtcgctacca
     4081 ggagctggcg ggggcccccg gcctgttcgc ccgtcacctg ccggtgcagt gcggcctcga
     4141 accctccagg actgtctccg cggagctgga gcgcatcgcg gaggccacgg ccgagcaccg
     4201 ggattggcag gactgcttcg actggaccgc cctcggcctc gcgggcgatg gcacggcgcc
     4261 gccgtttttc cccttctgct tcgagctcga ggagcgcgct ccgctggcag aggctggtgg
     4321 gttgcgcgtc gagaggctgc ggcagagcgt atgggccgac cggttccagc tcaagctcac
     4381 ctgcgtgcgg gagggcgccg gcatgacggc ggagctgcag ttcgacgcgg ctgtcctctc
     4441 agccgccgcg gtcggccggc tggccgccca gctcgctcgc ctcctcgaga gcgccatcga
     4501 gaaccccgga caacccatcg gcgctctcga tctcctggcc tcttggactg ctcacgagtt
     4561 gctggtggaa gacaacgaca ccgccctcga gttgggggcc gaccgctgtc tccacgagtt
     4621 ggtggaagag caggtggcga agaccccctc gcacaccgcg gtgatcttcg aggggcagga
     4681 cctgacctac gaggagctca acgtccgggc caaccggttg gctcacgcgc tgcagcggat
     4741 cggagtcggg cccgacgtgc gggtggccgt ctgcatggag cgcgcgcccg agatggtcgt
     4801 cagcctgctt ggcatcctca aggcgggcgg tgcctacgtc ccactcgatc ctggctaccc
     4861 cgcggatcgc ctggagctga tgttgggcga tagcggagcg gcgctgctcc tcacccagga
     4921 gcgtctgctg ccggttctgc cggagagcgg cctgccccgt atctgtgtcg acggcgaggc
     4981 ggagctccac tgggaggaag gcgccgagaa cccgcgcgtg ccggtggagc cgcggaacct
     5041 ggcctacgtc atctacacct ctggatccac aggccgtccc aagggggtga tgattcccca
     5101 cgccgcaatc aacaaccgcc tcctatggat gggccggcgc ttcggattgg gcgaagggga
     5161 gcgggtccta caaaagactt cgttcagctt cgacgcgtcg gtatgggagt tgttcctgcc
     5221 gctgctgacc ggcggcatcg tggtcctagc gcggccgggt gggcagcaag acaacgccta
     5281 catggcgcgg accatcgccc gggagggcgt tagtgtcctt cagctcgtcc cgtccatgct
     5341 ccgcctgttc ctggaggagc ccgaggtgaa cgcttgtggc tcgttgcgcc acctgttttg
     5401 cggcggcgag gcgctgtcgc gcgacctgca ggagcgctgc ttcgaacgtc tggacgccga
     5461 gctggtcaac ctctacggcc cgacggaagt ctcgatcgac gccacctact gggtctgcgg
     5521 ccgcgggagc gacctgcccc tcgtccctat cggctcaccg ctgtccaaca tccgcgtgct
     5581 cctgctcgat gccgagatgc gcccggtacc gtttggcgcc accggtgagg tctgcgtcgg
     5641 cggtgccggt ctggcgcgtg gctattgggg tctgcccggg actacggccg agaagttcgt
     5701 gcctgatcct ttcgcgccgg agcctggcgg caggctctat cgcaccggcg acctgagccg
     5761 tttcgtggct ccgggcgtgg tggcgttcgc ggggcgcgtc gatcaccagg tgaaggtgcg
     5821 gggcttccgc atcgagccgg gagagatcga ggcggtgctg cgcagccacg aggcggtgcg
     5881 cgatgcggtg gtggtggcgc gcaaggacgc ggccggcgac cacctagtcg cctatgtggt
     5941 gccgcgcgag accgactcgc tctccgatct gaggcggtgg ctgggcgagc gcctgcccga
     6001 gcacatggtg ccggcgttcg ttgtcgagct tgcctccttt cccctgctgc ccaacggcaa
     6061 ggtggaccgg gccgccctgc cggctccgga gccggagcgg cggagcgtcg aggcctatct
     6121 cgccccccgc acgtccacgg aggaggtgct ggcgactctc tgggaggacc tgctgggctg
     6181 caagcgggtc ggggtccatg acgacttctt cgacctggga ggacactccc tcctcgccac
     6241 ccagctcgtc tcgcgtttgc gccagggtat gggaatcgag ctgtcgctgc gcgccatctt
     6301 cgagaccccg accctggctg gcttggccac cgaggtggac cacgtccggc gcggagggga
     6361 gacctcagag gctccgccga tccggccggt gccgcgagac cgctccctgc cgttgtcctt
     6421 cgctcagcag cggctctggc tcctcgaccg cctcgcgccg ggtagcgctg cttacaacct
     6481 gcccatgggg ttgcgcctgg aggggccgct gcaggcgtcc gccctgacgc gtgccttgtc
     6541 cgagatcgtc cgccgccacg aggccctgcg cacgaccttc gccgagttgg acggtgagcc
     6601 ggtccagttg atcgccgccc cggcgccgtg gcacctcccc gtgatcgacc tggacgccct
     6661 gccggtggaa cgccgggagg gtgtctccca ggagctcacc cgtgcccagg cgcgccgcgg
     6721 gttcgacctg gtgcgtggcc ccttgttgcg cgtcctgctg ctgcgcctcg gaccgggcga
     6781 gcacgtgctg ctcgccaccc ttcatcacat cgtctccgac ggctggtcta ccggcatcct
     6841 agtgcgggag ctggcccacc tctacgagag ggccgtcgag agacagccga cgcgattgat
     6901 ggagctgccg gtccagtacg ccgacttcgc cgtctggcag cgcggctggc tgcagggtga
     6961 agtcctggag gctcacctcg actactggcg ccaccaactc ggcgctgaga tcccggtgct
     7021 gcaactcccc accgaccggt cgcgtccagc agtgcagacc acgcgcggag cggtcgaggc
     7081 agagtggctg tcgccccggc tgacgaatga gctggagagg ctgagccgcg gagaaggggt
     7141 gaccctcttc atgacgctgc tggcggcctt ccaggcgctc ctggcccgct acagcggtca
     7201 gtcggacatc gtggtgggct cgcccgtggc caaccgccac cgtctcgaga tcgagagcgt
     7261 catcggctgc ttcatcaaca cactggtgct gcgcaccgac ctgtcagccc tcggcagctt
     7321 ccgcgagctg ctggggcagg tacgggaggt ggcgctcggc gcctcggcct tccagggctt
     7381 gcccttcgag aagctggtgg aggagctgca gccgcgacgc gacctcagcc gttcgccgct
     7441 gttcgacgtg ctgttcatgc tgcagaacgt gcccttcgag caactgcagc tgcccgggct
     7501 cacgctttcg ccgatgcggg tggatgccgg cagggcgccg ttcgattgga ccctctctct
     7561 ggcgccgaca gagcgcgggc tcctcgcctc cctggagtac aaccgggacc tgttcgatgc
     7621 tgctacggcc gcccgggcgc tacgccatta cgagcgcctt ctcactacct ggacccagac
     7681 tcccggcgcg gacgtctggg agacgcccct tctttcgccc gccgaggagc accaggtcag
     7741 gaccgagtgg aaccgatggg gcgacgggtt ccccaccggc gtcggcgttc accagttgat
     7801 cgcggaccag gcgcgacgta cgccggaggg cgtggccgtg gtcttcgagg gagaggtgtg
     7861 gacctaccgc gatctcgcgg accgcagcgc cgcggtggcg cgccggctgc gcgccctggg
     7921 ggccgggccc ggcgccctgg tgggcctgtt cgtcgaccgc tccgccggga tggtggctgc
     7981 gctgctgggg accttggagg ccggcgccgc gtacgtgccc ctcgatcccg cccagccgcg
     8041 cgagcgcctc gccctgatgc tggaggaggc cactcccgcc gtgatcgtct cccaggacca
     8101 tctgcgcggg gatctgccgc cggtcggcgc gccggtgctc ttcctcgagg agatcgggga
     8161 gggtgaagat tccggcggtg taccggtgcc ggccttccag cctacggggg acgagcccgc
     8221 ctacgtgctc ttcacctccg gctccacggg ccggcccaag ggtgtgcagg tgccgcacag
     8281 ggcgctggtc aacttcctct gctcgatgcg cgagtgcccc ggcatccact ccggggatgt
     8341 ctggctggcc gtcaccccca tctccttcga catcgcgggg ctcgagcttt atctgccgct
     8401 cctcgctggc gcccgcgtgg tcctggcaag ccgcgagacc gtggtcgacg gcgcccggct
     8461 gctctccctg ttgagagagt ccggagcgac tgtcatgcag gcgaccccgg ccgggtggcg
     8521 tctgctgctc gaggcgggct gggggagccc ggatctgacc gtgctctgcg gcggcgaggc
     8581 gttgccgcga cagctcgcgg aggatctcca cgcccgcgcc cgggaggtct ggaacctcta
     8641 cggccccacc gagaccacca tctggtcgac ggtggagcgg gtgaagcctg gagccggcac
     8701 ggccgccgtc gccatcggcc tgcccatcgc caacacccag gtccacgtgc tcgatcgggc
     8761 gctccgcccg gtgccggcgg ggctcgctgg cgagctctgc atcggcggtg tgggcttggc
     8821 gaccgggtac ctgcggcgcc ccgacctgac ggcggagcgc ttcatccctg atccctgcgg
     8881 ctctgatgcc ggggcacgtc tctaccgcac cggtgacctg gcgcgccgcc tagccgacgg
     8941 ccggctcgag tacctgggac ggctggacca ccagatcaag gtgcgcggtt tccgtatcga
     9001 gctcgaggag atcgaggcgg tgttggccag acatccggaa attcgccaga ccgtggtgct
     9061 ggcgcgccag gacacgccgg gagagccgcg gatcgtcgcc tacctggtgg gggacggggt
     9121 ggtgaacgac gccgggctgc ggactttcct caaggatcgg ctgcctgact acatggtgcc
     9181 gtccgcgttc gtccggcttg cggcgctgcc cctcacgcca aacggcaaga tcgaccgccg
     9241 cgcgctgcct ccaccaccgg ccgagcgtgg cggcgacagc gcgctgcccg cgactccagt
     9301 ggcggaggtc ctggcggggc tgtggaccga gctcctcgcg gtcgatccta tcgggctcca
     9361 cgacgacttc tttgccctcg gcgggcactc gctgatggcg gcacggctga tcgcccgcgt
     9421 gcgggagacc ttcggcgtcg agctgtccct gcgccgggtg ttcgaggcgc ctacgctcgc
     9481 cgccctggcc gcggccgtgg acgccgctct gcaggagggt aggggagcgg tggcacctcc
     9541 tttgcggcgc atgccttcac tagctcggga aggtggagca cccctctcct tcgcccagca
     9601 gcggctctgg ttcatcgacc agctccagcc cggcagcccg gcctacaacc tgccggtggt
     9661 gctccgcctg agtggcgacc ttgacgtcga ggcgctgcgg gaaaccctgg cggagatcct
     9721 gcgacgccac gagatcctgc gcaccgtcta cccgatggcg gaagatggcg ggccccgggt
     9781 caccgtctcc agtgaggtgg ggccggccct gtccgtcctc gacctgcggc cggtgcccgc
     9841 cggtgagcgg gaaggggagg cgctccgcct cgcccgggcc gaggtgcgac gtccattcga
     9901 tctcgccgcc gggccgctga tgcggaccct gttgctgcgc ctcggcgaag ccgaccacgt
     9961 ggctgtgttc accatccatc acatcgtctc cgacggctgg tcgatgggcg tgctggtccg
    10021 cgaagtaggg cagatctatc cggctctgct cgcgggactg ccgtcgcccc tgccggacct
    10081 gccgctgcag tacgccgact ttgccgtctg gcagcgggag tggctgcagg gcgaagtgct
    10141 tgccgagcgg acggcttggt ggcgggagca actcgccggc attccagccg tgctcgagct
    10201 gccggccgac cgcccgcggc cggcggtcca gagccaccgt ggctcgacgc tccccttcgc
    10261 cctctccgcc ggagccaccc gggacctccg gagcctgggc cgtagcaggc gggctaccct
    10321 attcatgacc ctgcttggcg gcttccagac cctgctctat cgctataccg gccaaagcga
    10381 cgtggtggtg ggtacgccgg tggcgaaccg cgaccgctcc gagttggagg ggcttatcgg
    10441 cttcctggtc aatacgctgg tgctgcgagc ccgcttcgag ggtgtcccga ccttcggcga
    10501 tctgctcggc cgcttgcggg agacggcgct cgaggcccac gctcggcagg agttgccgtt
    10561 cgagaagctg gtggaggagc tgcagccgca acgcgacctc agctactcgc cgatcttcca
    10621 ggccatgctg gcgctgcaga acgcccctgc cgaggcgttg tccctgccgg gcctcgaagc
    10681 cgaacccctt gacgcccggg ggaactccgc caagttcgac ctgctgctga ccctcaccga
    10741 gtcggaggcc ggcctgtccg ggtcaatgga gtacgccacg gacctgttcg aagccgcgac
    10801 gatcgagcgt ctgctcgggc acctccgggt gctcctcgaa ggcgccgcgg ccgatcccgg
    10861 cctgcacgtc tcggatttgc cgctgcttac cggcgctgag gagcggcagg tggtgcggga
    10921 gtggaacgac accgcgttcg ctatcccaga cgagttctgc ctgcaccagc gcattgaggc
    10981 ccaagctggc ctgacgcccg acgccacggc cgtggtgtgg cgcgacctga gcctgaccta
    11041 ccgggaactg aacgcccggg ccaaccggct cgcctgtcac ctgcgagccg ccggagcggg
    11101 acccgacggc cgtgtcggcg tctgcctgga gcgctcggag gccttggtga tcgcgttgct
    11161 ggcggtcctc aagtccgggg ccgcgtacgt ccctctcgat cctggctacc cggacgagcg
    11221 gctcaccttc atgctgcagg acgcccggcc ctcggtctgc atcaccgacg aggcgatccg
    11281 gccccgtctc acgggcgccg tgccgctgct ctgcctggac acgcagcgcg aggaggtggc
    11341 gcggtggagc ggcaaggacc tggccgcttc ggcaacgctt gataatctgg cctacgtcat
    11401 ctacacctcc ggctccacag gccggcccaa aggggcgatg atcccgcacc gcggcatctg
    11461 caaccgtctg gtgtggatgc aggcacagta cggcctcacc ggcggcgacc gggtgctgca
    11521 gaagacgccg ttcagcttcg acgtttcggt gtgggagttc ttttggccgc tgctggccgg
    11581 cgcctgcatc gtggtcgccg agcccggagg gcaccgcgac agcgcttacc tggttcgtac
    11641 gatcggcgag caggaggtca cgatcctgca cttcgtgccc tccatgctcc aagccttcct
    11701 ggaggaagag catctggagg cgtgtgacgg ggtgcggctg gtggtgtgca gcggtgaggc
    11761 gctgcccctc gacctgcaga aacgtttctt cgaggtcatg ggtgcggagc tgcacaacct
    11821 gtacggaccc accgaagcct ccgtggacgt cacctatcac gcctgccgcc gcgggacgcc
    11881 actgcgctca gttcccatcg gccgcccgat cttcaacacc cagatctaca tcgtcgaccc
    11941 gggcctccgc ccgttgccgg tcggggtggc gggagagttg cttatcggcg gcgacggcgt
    12001 cggccggggt tacctggatc gcgccgcgct gactgccgag acgttcatcg ccgatccgtt
    12061 ctccgctgcg cccggggccc gcctctaccg cacccacgac ctggcgcgcc tgctgcccga
    12121 tggggctatc gagttcctgg gccgcatcga caaccaggtc aaggtacgcg gcttccgcat
    12181 cgaactgggg gagatcgagg cggtgctggc ggagcacccg gcggtgcgcg aggcggcggt
    12241 gatggcccag cagggtcgca gcggtgctcc ttcgctggtg gcttggttcg tcgtgcagga
    12301 cgagccgctg tccgtcgacg gcctgcgcgc cttcctgcgg cagcggctgc ccgaatacat
    12361 ggtgcccacg acgttcgtgc ggctccccgc gctccccttg aacgccaacg gcaagctgga
    12421 ccgcaaggtt ctgccctccc tcgcggggga gagggtagag ccgggccgca cctatgcggc
    12481 gccttccacc tacctcgaga agacgctggc ggagatctgg gttcaggttc tcaaggtcga
    12541 ggagatgggg gtccacgaca acttcttctc tctggggggc gactccatcc tgagcctgca
    12601 ggtgacggcc caggcccggc ggcgggggct ccgcctggcc ctccagcaga tctttatgca
    12661 ccagaccatc gcggagctcg ctcgtgccct cgaggagaca cctgcagaga caggcgcggt
    12721 gacggtcacc gacagcgggg acgagccctt cggcctgctc tcgccggagg accgccgcaa
    12781 gcttcccgat ttcgtcgagg acgcctaccc gctgaccatg ctgcaggccg ggatgctgta
    12841 ccacatgggg ttgaatcccg acgccccggc ctaccacaac gtcaacagcg tccacctgcg
    12901 gggccgcttc gaggcggcta ctttccagga ggcggtgctg caggtggtgg cgcggcacgc
    12961 ggcgctgcga acctccttcg accttgcggg ctacagccag ggcctacagc tcgtccaccg
    13021 cgcggcgttc ctgcccgtgg cagtcgaaga cctgcggtat ctcaccgcca ccgagcagga
    13081 gagagccatc gaagagctga tggagcggga gacgcggcgg ccgttcgatc tcgcccagcc
    13141 gccgttgctg cgcctgcacg ttttcctgcg gagcgacgac accttccagc tcacgctcac
    13201 tgagcatcac gccattttcg acggctggag cctccactcc acgctgaacg aaatcttcga
    13261 ccgctacttc accctgctga acggcgagtc cgtcccggag gccgctgcgc cggcgcctgg
    13321 cgcggcgttc cgtgattacc tgcggctcga aagcgatgcg gcggcctcgg cgtcgtcgcg
    13381 gcgcttctgg gacgagaagc tcagcgactg caccgttatg gagctgccat cctggcacgc
    13441 cactgcgcgc ggctcgcgtc cggcgcgggt cgagatgctc cctgtgccgg tgccgcgcga
    13501 gatctcggac gccttgcagc agctctcgcg gtccacaggg gtgccgctca agagcgtgct
    13561 gctggcggtg cacctcaagg tgatgagcgt gctctggggg caggaggacg tgctcaccgg
    13621 catgggcact cacggacggc ccgaggaggc ggggggcgag gacgtccgcg gtctcttcct
    13681 gaacatggtg ccctgccggt tgcgcgtccg ctcggggcag acctggcgcg agctggcgcg
    13741 gaacgccttc gagctggaga gggagctgat ccctcaccag cgctacccgc tggcggcgct
    13801 gcagaagagc cggggcggcc agcgtctctt cgacacgctg ttcaacttcg tgcacttcca
    13861 cgtcgtcagc gacatgctcc agtcgggacg gctggaggtg ctcggcttcc gcaagtacga
    13921 ggcgaccagc ttccggctgc tgacgctgtt cgcgatcagc ctggtgaagt cgcaggtgac
    13981 gctggagctc gatttcgacg gcgccgagct gacccgggag caggtctcga ccatcggcga
    14041 tctctatgcc cgggctatgg cggccatggc tgaggcgcct gaccagagcc acgacgtgct
    14101 gctcccgctt tccgaggcgg cccgccatca ggctctggtg gagtggccgg acactgccac
    14161 cgcgtacccg cgcgaagccg ctgttcccga gctgttcgag ctgcaggcgg cccagacccc
    14221 cggcgccatc gccgtagagt tcggtgccga ccggctgacc tatgatgagc tcgatcatca
    14281 ggccaaccag ctcgcctgca gtctgcgccg gctcgaagtc ggtccggagg taccggtggc
    14341 ggtcttcatg gagcggtcgc accggatggt ggtgaccttc ctggggatcc ttaaggcggg
    14401 cggctactac gtacccctcg atccgagcta cccccgggag cggttggagg tcatgctcga
    14461 cgactcgggc gcgcgggtgc ttctgaccga ggagcggctg gcgtcccttc taccggctcc
    14521 gggcctcacc gtcttgcggc tcgacagcca gtgggaggag gtcgctaggg agagcggcgc
    14581 gccgctggac cggcggagta gcgcggacaa cctcgcctac gtcatctata cctccggctc
    14641 gaccggcagg cccaaggggg tgtcgatccc gcaccgcggt atcgtgcggc tggtcaaggg
    14701 cacgaactat gtgcaggtcg gagccgatga ccacgtagcg caggcctcca acacctcgtt
    14761 cgacgcggcc accttcgaga tctggggcgc cctgctcaac ggcgcccgcg tcgtcggtat
    14821 cccgcgcgag gtggcccttt cgcctcagga cctcgccgct accatccgtg accggcggat
    14881 cacggtcctg tttcaaacca cggcgctgtt caaccagacc gtgcggctgg tccctgacgc
    14941 gtacgccggc atccgccggc atctggtggg cggcgaggcc ttcgatccaa gggcgttcga
    15001 agctctccag ctcggcggcc ggccgccgga gcggtttgcc aatatctacg gtccgaccga
    15061 gagcactacc tacgcgacct ggtaccccat ggaccggctg cccgacggca cctcggttcc
    15121 gatcggccgg cccatcgcca acaccacaac gtacgtgctc agcgaagccg ggcagccggt
    15181 ccccatggcg accccgggcg agttgcatat cggcggcgac ggcctcgctc gcggctatct
    15241 cggccggccc gagctgacgg cggagaagtt cgtgccccac cccttcgcgg cgacgccggg
    15301 ggagcggctg taccggaccg gcgacctcgt ccgtccgctg tctggcggcg acatcgagtt
    15361 ccttgggcgc cgcgatcttc aggtgaagat ccgcggcttc cgcatcgagc tcgacgagat
    15421 tcgtatcgtc ctcgagcagc acccggcggt ggtcgaatcc ttcgtcatgg ttcgagagga
    15481 tcagcccggc gagcggcggc tggtggccta tttcgttccg gtctcgggcg gggcgtccga
    15541 gatccaggcg ctgcgggagc acctgcgcga gaagctgccc gactacatgg tgccttccgc
    15601 gttcgtgccg cttgatgcgc ttccaatgtc ggtcaatggc aaggtggacc ggcggctgct
    15661 gccgactccg gactggagcc agccggagaa gcaggagagc tacgccggcc cccgcgacgc
    15721 cgtcgaggag gcgctggtgg agatctggca gcaggttctc ggtgttgccc ggatcggcat
    15781 ccacgacaac ttcttcgagc taggcggcga ctcgatcctg agcatccaga tcgtcgcccg
    15841 agccggcgaa aaggggctcc ggctgacccc gggcaacatc tttcagcacc cgacggtggc
    15901 ggagctcgcc atgatcgcgg aagctgccga gaccgccact agggcctccg cctccgcctc
    15961 cggcacgggc gaggtgccgc tgaccccgat ccagacctgg ttcttcgagc aggatctgcc
    16021 ccggccgcat cacttcaacc agtcgatgct gctcgaggtg ctggcaccgg taccgccggc
    16081 cgcgctggcg accgtggtgg agcacctcgt cgaacagcac gacgcattgc ggctgcgctt
    16141 tgagaggacc gcgtccggtt ggcggcaggt ccaggccccg tccggcggct gcacgtctct
    16201 gtcacgggtc gacctcgcgg cactgccggc ggagctctgg aaggccgagg tagaggccgt
    16261 ctgcgcccag gtccagcaga gtctcgacct cacccgtggc cctctgcttc gtgccgtgct
    16321 catcgatcgg ggcgccgccg gcagcgcgcg cttgatgatg gccgtccatc acctggtggt
    16381 ggacgccgtc tcctggcgcg tcctgctgca ggacctccag gtcgcctacc ggcaggtcgc
    16441 ggccggtggg gtcgccctcg tgcccgccag gaccacctcg ttccagcact gggcggagcg
    16501 gctgcgggag tacgcggcaa cggcggccat ccgccaggag ctttcctact ggaccgaccc
    16561 ggcgcgggct gaggccgcgt tcgtgccccg cgactttctg gagggggaca acgtcaagga
    16621 gtcgatccgc gccgtggcgg tctggctcga gccggacgag acccagcaac tcgtgcagga
    16681 ggtgcccagg gtctaccaga cacagatcaa cgacgcgctg ctcaccgggc tggcgttggc
    16741 gttcgcccgc tggaccggcg agcggtcgct gctcgtggat ctcgagtcgc acggccggga
    16801 ggagatcttc cccgacgtgg acctgtcgcg caccgtcggc tggttcacca atctcgctcc
    16861 cgtcctcctg cgcgtggagg agggcctgga gccggaaggc tcgctcaagg cggtgaagga
    16921 gcagctccgt gcggtcccgc gacgcggcat cgggtacggg ctgctgcgct acatggcgcc
    16981 tgagcccggg gtcgccggcg aactgcgccg gctcccgcgg gctcaggtgg gcttcaacta
    17041 cctcgggcag gtggaccagg tgctcgccga gtcgccgctg ctgcgcccgg cccccgagtc
    17101 ctcaggcagg ccggagagcc gcagcgggct gcgtccccac cttctggaaa tcacggcggt
    17161 gatcgtcgag ggcaggctgc gcgtccgcct cgaatacagc aagaacctcc atgagcgctc
    17221 gaccatcgac acgctcggcc gttacttcat ggaggcgctc cgctccatcg ttgcccactg
    17281 cctgactgcg gggacggttg gatacacgcc ctcggacttc cccagggcca gactcaaaca
    17341 gaaagatctc gaccagttcc tgaccaagct cggggggtcg cgaaagggag aagcggttga
    17401 agtctgagga gatcgacgat atttacgagc tgtccccgat gcaggcgggg atgctgttcc
    17461 atagcctcgc ggccgagagg tccggggtgt atgtccttca gttcgtctgt ccgctgaccg
    17521 gagggctcga tgaaaccgcg ttcgagcgcg cctgggaggg gctggcggca cgccatgccg
    17581 tgctgcgcac ctcgttccat tggcaggagc tggacaagcc cttgcaggtc gtccaccaga
    17641 aggtggcgtt gccactggcc cggctggact ggcggagcat tccggaggaa gcgcaggagc
    17701 agcgcctgcg ttcgctgctg cgcgaggatc gcgtccaggg cttcgatctc tcgcaggtgc
    17761 cgctcgtgcg gctgctcttg atacggaccg gggataccgc ctatcggctc ctctggacca
    17821 cgcaccacat cctgctcgat gggtggtgcc tggggctcgt gctccaggag ctcctggagc
    17881 tgtacgacgg gtttcggcgc ggagagccgg tccgcctgcc gccggttcgg ccctacgccg
    17941 attacattga gtggctgcag agtcaggacg ccgccgcagc cgaggcattc tggcgccgca
    18001 tactctccgg cttcggggcg gcgacgcgct tgggcgtgga ccgcctggcg gcaccggccg
    18061 cttcggacgg cgcagccctc gacccgggtg gcgaggccgt gtttgccgag gagtccc
//
LOCUS       DASPJI010000669         5912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_154018, whole genome shotgun sequence.
ACCESSION   DASPJI010000669 DASPJI010000000
VERSION     DASPJI010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154018"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VEL74_18995"
     CDS             complement(<1..356)
                     /locus_tag="VEL74_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64675.1"
                     /translation="MFPDNILPYDVDELPTVLPVFPLPAPLLLPGSAAGLYTADPGQI
                     TMIQETLEGDGWVGIVQPQTAGFSDDDEPVLCSDPDAPLFSVGCLGYLGEPGEDEDEH
                     MAGVVGGVIRFRVVEEL"
     gene            complement(474..2342)
                     /locus_tag="VEL74_19000"
     CDS             complement(474..2342)
                     /locus_tag="VEL74_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG64676.1"
                     /translation="MGAAPPGGTPPAPRSMRERFGALRNLPPFLKLIWQTSPALTAAD
                     LLLRLVRALLPVATLFVGKLIIDEVVRLAGIPSAPATLREWLASGRLAHLGWLLAAEF
                     GLAVLSDVLGRTVSLIDSLLSEQFTNATSVRLMEHAATLDLEDFEDSELQDRLDRARR
                     QTTGRMALMSQLFGQAQDVVTIIGFAAGLVVYAPWLIALLILALVPAFLGEAHFNAQS
                     YSLNYARTPERRELDYVRQTGASVESAKEVKIFGLNAFLIDRYRALAGAIFQENRRLA
                     TRRAGWGGLLTAVGTLGYYVAYAFIAWRTLHGDFSIGDLTFLSGSFRRLRNLLEGLLI
                     GFSQVAGQALYLDDLFSFFEIQPEIVSPENPRPFPVPVRQGFTFEDVGFRYPGAERWA
                     VRHLSFHLRAGEVLALVGENGAGKTTLVKLLARLYDPDEGRILLDGHDLKEYDLEALR
                     ANIGVIFQDFVRYHLTAAENIAVGRIEARGDRARIEDAAERSLADEVIRKLPEGYDQV
                     IGKRFRGGVDLSGGEWQKIAIARTYMRDAQLLILDEPTAALDARSEFEVFQRFKELSE
                     GKTAVLISHRFSSVRMADRILVLAEGQIEAMGTHEELLAQRGRYAELFELQAAGYR"
     gene            complement(2480..3334)
                     /locus_tag="VEL74_19005"
     CDS             complement(2480..3334)
                     /locus_tag="VEL74_19005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64677.1"
                     /translation="MRFRWLAVFPVAWGVTFLVLAAALSGVSSYGIFLRTEIELVKIL
                     ALLGSWGAALAFEKGEYMRRAWFLIGAAFACFLLRDLTLAPWGFEEALGTTGLLLLRG
                     ALSILGNLSQVVGTWMLARVWKVSGLSLPGQRSRQILVLLGAMALAAVFAGPAVVEHG
                     GKVLAGGWEAIPLLASALGDAVVLCLIAPLLLTALALHGGYLEWVWGLLTASSLAWLL
                     YDGVQVFGPNLGLSTDAARVASELFRALGCTLGFSAGVAQRWIVVQVQGLTRETAEAG
                     TAVSAPRM"
     gene            complement(3334..3573)
                     /locus_tag="VEL74_19010"
     CDS             complement(3334..3573)
                     /locus_tag="VEL74_19010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64678.1"
                     /translation="MDKQTVAEQVAAQLAPYLGPFNASNAVRTWSERKLLLSPEDLRP
                     EHVPKLLEALRPMLCTFVGRSSATVLVEKIGREVR"
     gene            complement(3715..>5912)
                     /locus_tag="VEL74_19015"
     CDS             complement(3715..>5912)
                     /locus_tag="VEL74_19015"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HYG64679.1"
                     /translation="AQDSAKLAIGTKEGLYGRLADGMRERLGGQGVELTIEETSDCLT
                     SLERLHKGDVRFALAPQDVVSEYFRGNRDTKVRVVDRVFFDYLHIFLRESLHINTLSD
                     LARLRVWAGEEKSGTHLAAARFFDSLGIPLATLGDRHLVWDAPPDAWDNLPNWFRNDR
                     LDVAILAASPGTPSICRLMASGTCSLLPLDANTLRRLGYEARTDGQDRYRRQISVGEI
                     PKGTYANQRGSIATIAIPVLLLAHADERTALAARLRDAAQEEWREAVERPGPNGCRIP
                     ENLPEAEPLRESGLRLLRVPHPPEPPLARLSRAVTPILPFLLFLAAGTLLFLQRRRCR
                     LLVRYLWNHYRVLCVIGLVLGGSVLIITLSAFHVEHQINENFSSVWESFWSITIYLFS
                     GLEDRVPYTTAGRILVTLGLLVGAVCSTVASGWTASLFIRREKRMPQNLRNHFLMLNW
                     NERAAAIVRQLHHPILRRQDGVSVIVILTDDESLTVQKLKEAGSGRDPAFEDFFLSIG
                     DPTEELALLNANAQDARTIVVLADEKHGDERALRSVFMLRRIAKQKDVKLHVVVELLE
                     AANDCMVEELAKDFPGSLELVSGLQIRTLLLAQAALNTGIVGFYTDLLRISGDSNEVY
                     TQEIPETAVGMRFREYASLVVGASSATPLIPVGVQRQVNGKPQILTNPQPKTPGDILQ
                     PGDRLLLIAYGPPERNALPVPERSRAARRARPSLSPPETPEPASAGSLP"
BASE COUNT         1018 a         1864 c         2107 g          923 t
ORIGIN      
        1 agctcctcga ccacccggaa gcggatcacc ccgcccacga cgcccgccat gtgctcgtcc
       61 tcgtcttccc ccggctcgcc caggtagccg aggcagccga cggagaacag cggcgcgtcg
      121 gggtcgctgc agaggaccgg ctcgtcgtcg tccgagaacc ccgccgtctg cggctggacg
      181 atgccgaccc agccgtcgcc ctccagggtc tcctggatca tggtgatctg gcccgggtcc
      241 gcggtgtaca ggccggcggc cgagccgggg agcaggagcg gcgcggggag cgggaagacg
      301 gggagcaccg tcggcagctc gtccacgtcg tacggcagga tgttgtccgg aaacatggtc
      361 ctctcgatct tctcaaggcc cgcggcgcga tgcaacaggg gcgggctcgt tcatctatgt
      421 atacggatga cgagggcgat ccattcctgc gggggcgacg atagccggct gtctcagcgg
      481 tagcccgcgg cttgcagctc gaacagctcg gcgtagcgtc cgcgctgcgc cagcagctcc
      541 tcgtgggtgc ccatggcctc gatctgcccc tcggcgagga ccaggatgcg gtccgccatc
      601 cgcacgctcg agaagcggtg ggagatcagc accgcggtct tcccctcgct cagctccttg
      661 aaacgctgga acacctcgaa ctcggagcgg gcgtcgaggg ccgcggtggg ctcgtcgagg
      721 atcaggagct gggcgtcccg catgtaggtc cgggcgatgg cgattttttg ccactcgccc
      781 ccggagaggt cgacgccgcc gcggaagcgc ttgccgatga cctggtcgta gccctccggc
      841 agcttgcgga tcacctcgtc ggcgaggctg cgctcggcgg cgtcctcgat gcgcgcccgg
      901 tcgccgcggg cctcgatccg gccgaccgcg atgttctcgg cggcggtcag gtgatagcgc
      961 acgaagtcct ggaagatgac gccgatgttg gcgcgcagcg cctcgaggtc gtactccttg
     1021 aggtcgtggc cgtcgagcag gatgcgcccc tcgtcgggat cgtacagccg tgccagcagc
     1081 ttgaccaggg tggtcttgcc ggcgccgttc tcgccgacca gggccagcac ctcgccggcg
     1141 cgcaggtgga agctcaggtg gcggaccgcc cagcgttccg cgccgggata acggaagccg
     1201 acgtcctcga aggtgaagcc ctggcggacc ggaaccggga acggccgcgg gttctccggc
     1261 gagacgatct cgggctggat ctcgaagaac gagaacaggt cgtcgaggta gagcgcctgg
     1321 ccggccacct gggaaaatcc gatcagcagg ccttccagga gattgcgcag ccgccggaac
     1381 gagccggaga ggaaggtgag gtcgccgatg ctgaagtcgc cgtgcagggt acgccaggcg
     1441 atgaacgcgt acgccacgta gtagcccagg gtaccgacgg cggtcaggag acccccccag
     1501 ccggcgcggc gggtggcgag ccggcggttc tcctggaaga tggcgccggc gagggcgcgg
     1561 tagcggtcga tcaggaaggc gttgaggccg aagatcttca cttccttggc cgattcgacg
     1621 ctggcaccgg tctggcgcac gtagtcgagc tcgcggcgct cgggcgtgcg ggcgtagttg
     1681 agcgaatagc tctgggcgtt gaagtgggcc tcgccgagga aggccggcac cagggcgagg
     1741 atcagcagcg cgatcagcca gggggcgtag accaccagcc cggcggcgaa gccgatgatg
     1801 gtcacgacgt cctgggcctg gccgaagagc tggctcatca gggccatccg gccggtggtc
     1861 tggcggcggg cgcggtcgag ccggtcctgg agctcgctgt cctcgaagtc ctccaggtcg
     1921 agagtggcgg cgtgctccat caggcgcaca ctggtggcat tggtgaactg ctccgacagc
     1981 agcgaatcga tgagggagac ggtgcgcccc agcacgtcgg acagcacggc cagcccgaac
     2041 tcggccgcca gcagccatcc gaggtgcgcg agccggccgc tcgccagcca ctcccgcagg
     2101 gtggcggggg cgctcgggat cccggccagc cgcacgacct cgtcgatgat cagcttgccg
     2161 acgaacaggg tggcgacggg cagcagcgcg cggaccagcc gcagcagcag gtcggccgcg
     2221 gtgagtgccg ggctggtctg ccagatcagc ttgaggaacg gcggcaggtt gcggagagcc
     2281 ccgaagcgct cccgcatgga gcgcggcgcc ggtggtgtgc ctccaggggg agccgctccc
     2341 atcgcccccc cagctccagg cgatgcgcgg cctcccatgg tgtagtgacg cggcattcag
     2401 gtggctgagt gcaggagggg tgccagcagc ggccgtcccc gcggaatccc gcgaggacgg
     2461 ccgccggtcc ttcgacgggt cacatccgag gcgcgctgac cgccgtaccc gcctccgccg
     2521 tctcccgcgt cagtccctgg acctgcacga cgatccagcg ctgggccacg ccggcggaga
     2581 agccgagggt gcagccgagg gcgcggaaga gttcggaggc gacgcgggcg gcatcggtgc
     2641 tgaggcccag gttcgggccg aagacctgga ccccgtcgta gaggagccag gccaggctgc
     2701 tcgccgtgag cagtccccag acccactcca ggtagccgcc gtgcagggcg agggcggtca
     2761 ggaggagcgg cgcgatcaag cagaggacca cggcgtcgcc cagggccgag gcgaggagcg
     2821 ggatggcctc ccagccgccg gcgaggacct tgccgccgtg ctcgacgacg gcggggccgg
     2881 cgaagacggc ggccagcgcc atcgcgccga gaagcaccag gatctgccgc gagcgctggc
     2941 ccggcaggct gagtccgctc accttccaga cccgcgccag catccaggtg cccaccacct
     3001 gcgacaggtt gcccaggatg gacagcgcgc cgcgcagcaa caggaggccg gtggtcccga
     3061 gggcctcttc gaagccccag ggggcgaggg tgaggtcgcg caacaggaag caggcgaagg
     3121 cggcgccgat caggaaccag gcgcgccgca tgtactcgcc cttctcgaag gcgagggccg
     3181 cgccccagct ccccagcagg gccaggatct tgaccagctc gatttcggtg cgcaggaaga
     3241 tcccgtagga ggagactccg gagagggcgg cggcgaggac gaggaaggtg acaccccagg
     3301 cgacggggaa gacggcgagc cagcggaatc tcatcagcgg acctcccggc cgatcttctc
     3361 gacgagcacc gtagccgagc tgcgtcccac aaaggtgcag agcatgggac ggagcgcctc
     3421 cagcagcttc ggaacgtgct cgggccgcag atcctccgga gagagcagga gcttcctctc
     3481 cgaccacgtc cggacggcat tgctggcgtt gaaggggccc agatacgggg cgagttgtgc
     3541 ggcgacctgc tcggcgaccg tctgcttgtc caaaggcaag tcctccccct tggttttgtc
     3601 gtcgatctgg gggaggagtc tacgcctgtc ggtgctgaaa aggaaggagg agatctcggg
     3661 attcgagcct tcaggtctgg cgtggttgca ctagttctct tgctggaata tctatcaggg
     3721 taaggagccg gctgaggctg gctccggggt ctctggcggc gagaggctag ggcgggcacg
     3781 tctggccgcg cgagagcgct ctggaacggg cagggcgttt cgctctgggg ggccataggc
     3841 gatgaggagc agccggtctc ccggctggag gatgtctccg ggggttttgg gctggggatt
     3901 cgtgagaatt tgtggtttcc cattcacctg ccgttgcact cccaccggga tcagcggcgt
     3961 ggctgagctc gcgccgacca ccagggacgc gtactctctg aaccgcatgc ccacggccgt
     4021 ctcgggtatc tcctgcgtat agacctcatt ggaatctccg gagatgcgga gcaggtcggt
     4081 gtagaagccg acgatgccgg tattcagtgc ggcttgagcc aggagaagag tgcggatctg
     4141 caggcccgat acgagctcga gcgatccagg gaagtccttt gccagctctt cgaccatgca
     4201 gtcgttcgcg gcttcgagga gttccacgac gacatgaagc ttcacgtctt tctgctttgc
     4261 gatccgccgc agcatgaata cggagcgaag ggctctctcg tcaccgtgct tctcatcggc
     4321 gaggaccacg atggtccggg cgtcctgggc atttgcgttg agcagagcga gctcctcggt
     4381 cgggtcgccg atgctgagga agaagtcctc gaaggcaggg tcgcggccac tcccggcctc
     4441 cttcagcttc tggaccgtca aggattcatc atccgtgagg atgacgatca ccgaaacacc
     4501 atcctgtcgt cgcaggatcg ggtggtgcaa ctgcctgacg atggccgccg cgcgctcgtt
     4561 ccaattcagc atcaggaaat ggttcctcag gttctggggc atgcgttttt ctctccgaat
     4621 gaagaggctg gcggtccagc ccgaggcgac cgtggagcag accgctccca ccaggagacc
     4681 gagggtgacg aggatccggc cggcggtggt ataggggaca cggtcttcga ggccgctgaa
     4741 caggtagatc gtgatcgacc agaaggactc ccacaccgac gagaagtttt cattgatctg
     4801 gtgctcgaca tggaaggccg agagggtgat gatcaagaca ctgccgccca gtaccaggcc
     4861 gatgacacat agcactcgat aatggttcca gagatatctc accagtaaac ggcagcgtcg
     4921 ccgctgaaga aagaggagtg tccccgctgc cagaaaaagc agaaagggaa ggatcggggt
     4981 gaccgccctg gacagcctcg ccagcggcgg ctccggcggg tgcggtaccc gcagcaggcg
     5041 gagcccggat tcccggagag gctcggcctc cggcagattc tcgggaatcc ggcagccgtt
     5101 ggggccgggc ctctcgaccg cttctctcca ctcctcctgc gcagcatcgc gaagcctggc
     5161 agccaaggcg gtcctttcat cggcgtgagc caggagcagg accgggatgg caatggtggc
     5221 gatgctcccc ctctggttgg cgtaggtgcc tttgggaatc tccccgacgg agatctgccg
     5281 gcggtagcga tcctgtccgt cggtcctggc ttcatagccc aggcggcgga gcgtgttggc
     5341 gtcgaggggc aggagagagc aggtcccgga ggccatgagc cggcagatgc tgggagtgcc
     5401 ggggcttgcg gcaaggatcg ccacgtccag tcgatcattc ctgaaccaat tggggaggtt
     5461 gtcccaggca tccggcggag cgtcccagac gaggtgacgg tctcccaggg tggcaagggg
     5521 aattcccagg ctgtcgaaga agcgggcggc tgccaggtga gtcccgctct tctcctcacc
     5581 ggcccagacc cgcaggcgtg caaggtcgct gagggtgttg atatgcaaag actctctgag
     5641 aaatatatgt agataatcga agaagaccct gtccaccacg cggaccttgg tgtcccggtt
     5701 gccacggaag tactcgctga cgacatcctg cggcgccagg gcaaacctca catctccctt
     5761 gtgcaggcgc tccaggctcg tcaggcagtc ggaggtctcc tcgatcgtga gctcgacgcc
     5821 ctggccgccg agccgctcac gcataccgtc cgcgagacgc ccgtaaaggc cttctttggt
     5881 accgattgca agcttggcgg aatcctgagc ac
//
LOCUS       DASPJI010000670         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_610647, whole genome shotgun sequence.
ACCESSION   DASPJI010000670 DASPJI010000000
VERSION     DASPJI010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610647"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VEL74_19020"
     CDS             <1..626
                     /locus_tag="VEL74_19020"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HYG64680.1"
                     /translation="GASGITVHLRMDRRHIQDSDVERLRASVGGKLNLEMSIAEEMVK
                     TALRIKPAQVTLVPERPEEVTTEGGLNLVFYGRRIGEVVDRLADAGIGVSLFLDPEPR
                     QIEAIATLAPRGIQGFEINTDAYTRAPGGLAADQELAKIAEVAELGRSHGLRLYAGHG
                     LTVDNVGPIAAITDMEELNIGYSIIARAVMVGVEAAVREMLEAIAAR"
     gene            694..1626
                     /locus_tag="VEL74_19025"
     CDS             694..1626
                     /locus_tag="VEL74_19025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG64681.1"
                     /translation="MELSVLLSTRDRAPLLASTLEHLCRQEGLPAGRWEVLVVDNGSR
                     DDTPAVLGRFASRLPLVALRQPQGGKSHALNRALEVARGALRVFTDDDVEPRPSWLAE
                     LLAASRRWPDHPVFGGRIIPRYPAETPDWLRDLVREHPEYASAAYAHYSLPQEEGPTE
                     DLPFGPNYAVRAQAMTGLRFCEDIGPVGKDYPMGEDTDMLIRLTRRGVPAIYVPGAMV
                     YHVVRPDQLQPSWLLGRAYRLGRGTLRLRVATLGQPLPPAWKAWLRLPLAWALYGRER
                     FRGPGRRLLAGVSIHFLRGYLLEHSRLRAERAGS"
     gene            complement(1623..>2836)
                     /locus_tag="VEL74_19030"
     CDS             complement(1623..>2836)
                     /locus_tag="VEL74_19030"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HYG64682.1"
                     /translation="GGDTRESTPRICRWLAEGLTAGGAEVRYAGVIPTPGVAYLTPVL
                     GAAAGIVVSASHNPYPDNGIKLLDPRGAKWSDDNEATLERRLLAGGSQPVPEMPEGAL
                     EPESGLREDYLRHLASTVPGETPLAGLRIVLDAGNGAASFYARELFERLGAAVTLLHA
                     APDGRNVNEKCGSTAPEEMAARVVAEGAHLGAAFDGDADRCILADDRGTVRDGDAILY
                     LWARHLRDTGHLQPPRIVATSMSNLGLERALDREGIGIKRCNVGDRYVVEAMREEGIV
                     LGGEQSGHIVQSELASTGDGLLTALQIAYIVHRSGRPLAELLGGFRRYPQILLNVRVA
                     RKTDFAALPRVVATQRCVEEKLGADGRLVLRYSGTEPLARVMIEGPDQPTIDALAGEL
                     AGVIREELGAG"
BASE COUNT          371 a         1031 c         1034 g          400 t
ORIGIN      
        1 ccggagcgtc cggcatcacc gtccatctcc gcatggaccg ccgccacatc caggacagcg
       61 acgtggagcg cctgcgcgcc agcgtcggcg gcaagctcaa cctggagatg tcgatcgccg
      121 aggagatggt caagaccgcc ctgcgcatca agcccgccca ggtgaccctg gtgcccgagc
      181 ggccggagga ggtcaccacc gagggggggc tcaacctggt cttctacggc cggcggatcg
      241 gcgaggtggt ggaccggctg gccgacgcgg gcatcggggt ctcgctgttc ctcgaccccg
      301 agccgcgcca gatcgaggcc atcgcgacgc tggcgccgcg cggcatccag ggcttcgaga
      361 tcaacaccga cgcctacacc cgcgctccgg gcggcctcgc ggccgaccag gagctggcga
      421 agatcgccga ggtcgcggag ctggggcgtt cgcatgggct ccgtctctat gccgggcacg
      481 gcctgacggt ggacaacgtc ggccccatcg ccgccatcac ggacatggag gagctgaaca
      541 tcggctactc catcatcgcc cgtgcggtca tggtgggcgt cgaagccgcc gtgcgcgaga
      601 tgctggaggc catcgcggcg cgttgaggca ttccccgtga ggcgccggca ggccttcggc
      661 gagcacccat gagggtggag ccggaggcct gagatggaac tgagcgttct gctttcgacg
      721 cgcgaccgcg cgccgctcct cgccagcact ctggagcacc tctgccgtca ggaggggctg
      781 ccggcaggcc ggtgggaggt gctggtcgtc gacaacggca gccgtgacga cacccccgcc
      841 gtcctggggc gcttcgcctc ccgcctgccc ctcgtcgcct tgcggcagcc gcagggcggc
      901 aagagccatg ccctgaaccg cgcgttggag gtggcccgtg gcgcgttgcg ggtcttcacc
      961 gacgacgacg tggagccgcg gccctcctgg ctcgccgagc tgctggccgc ctcccggcgc
     1021 tggcccgatc acccggtctt cggcggcagg atcatccccc gttatccggc ggagacgccg
     1081 gactggctgc gcgacctggt ccgggagcac cccgagtacg cgtccgcggc gtacgcccac
     1141 tattcgctcc cccaggagga ggggcccacc gaggacctgc ccttcggccc caactacgcc
     1201 gtgcgcgccc aggcgatgac ggggctccgc ttctgcgagg acatcgggcc ggtggggaag
     1261 gactacccga tgggggaaga caccgacatg ctcatccggc tcacccgcag aggggtgccg
     1321 gcgatctacg tgcccggcgc catggtctac cacgtggtgc ggccggacca gctccaaccc
     1381 tcgtggctgt tgggacgggc ctaccgtctc ggccgcggca ccctgcgcct gcgggtcgcc
     1441 accctgggcc agccgctgcc cccggcatgg aaggcctggc tccgcctccc cctggcctgg
     1501 gccctctacg gccgcgagcg cttccggggg ccggggcggc gcctcctggc cggcgtctcg
     1561 atccacttcc tccgcggcta cctcctcgag cacagccgcc tgcgggcgga gcgggcgggg
     1621 agctagccgg cccccaattc ttcgcggatg acgccggcga gctcgccggc gagggcgtcg
     1681 atggtgggct ggtccgggcc ttcgatcatg acgcgggcca ggggctcggt gccgctgtag
     1741 cgcaggacga gacggccgtc ggcgccgagc ttttcctcga cgcagcgctg cgtggcgacg
     1801 accctgggca gggcggcgaa gtcggtcttg cgggccaccc ggacgttcaa caggatctgc
     1861 gggtagcgcc ggaagccccc cagcagctcc gcgagcggcc ggccggagcg gtggacgatg
     1921 taggcgatct gcagcgccgt cagcaggccg tcgcccgtgc tcgccagctc gctctgcacg
     1981 atgtggcccg actgctcgcc ccccaggacg atcccctcct cgcgcatcgc ctccaccacg
     2041 tagcggtcgc cgacgttgca gcgcttgatg ccgatcccct cgcggtccag ggcgcgctcg
     2101 agccccaggt tgctcatcga ggtggcgacg atgcgcggtg gctggaggtg ccccgtatcg
     2161 cgcaggtgcc gggcccatag ataaaggatg gcgtcgccgt cccgcaccgt gccgcggtcg
     2221 tcggccagga tgcagcggtc ggcatcgccg tcgaaggcgg cgcccaggtg ggccccctcg
     2281 gccacgacgc gggccgccat ctcctcgggt gcggtcgagc cgcacttctc gttgacgttg
     2341 cggccgtccg gggcggcgtg gaggagggtg accgcggccc cgagccgctc gaagagctcg
     2401 cgggcataga acgaggcagc gccgttgccg gcatcgagga cgatccgcag cccggccagc
     2461 ggcgtctctc cggggacggt cgaggccagg tggcgcaggt agtcctcgcg caggccgctc
     2521 tccggctcca gggcgccctc cggcatctcc ggcacgggct gggagccgcc cgccagcagg
     2581 cgccgctcca gggtggcctc gttgtcgtcg ctccacttcg cgccgcgcgg atcgaggagc
     2641 ttgatgccgt tgtcggggta ggggttgtgc gaggccgaga cgacgatgcc cgccgcggca
     2701 ccgaggaccg gggtgaggta ggcgacaccc ggcgtgggga tgacgccggc gtagcgcacc
     2761 tcggcgccgc ccgcggtcaa tccttcggcc agccagcggc agatccgtgg cgtgctctcc
     2821 cgggtgtcgc ccccga
//
LOCUS       DASPJI010000671         9984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_723122, whole genome shotgun sequence.
ACCESSION   DASPJI010000671 DASPJI010000000
VERSION     DASPJI010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9984)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9984
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723122"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /locus_tag="VEL74_19035"
     CDS             <1..1493
                     /locus_tag="VEL74_19035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64683.1"
                     /translation="GLTQGNGKNNRDGLGATLELRAGSAYQFREAAGQVTHFGLGQRR
                     QADLLRVVWTNGVPQNRIQPSGEQRVVEEQVLKGSCPFLYTWDGERFAFVTDLLWNAP
                     IGLPVAPGKWVPPDPEELVRVDGARPDGGVYKLRITEELWEAAFFDHTRLWVVDHPAG
                     VEVASSLRVTPGGPPDGPDRVVASRGLRPVVSALDGTGADMTERVRERDEVYAGGYPE
                     SSYQGMSGEPWAFTFDLGEAPGRPLRLHLDGWIFPADASLNLAAAQRPDLAYVPPRLE
                     VETADGWQVLVPAMGHPAGKTKTLVVDTPPLPAGVSRLRIVTTLWLAWDRVAWSPEPV
                     DSEPVVRARLLPSEADLRYRGFSELVRRSPNGPHGYDYSRVASESPWLPFPGRYTRYG
                     EVRELLESPDDRTVVLAPGDEIALTFDASALPPLQEGWTRTVFLESHGWDKDADRNTW
                     EARQMEPLPFRGMSGYPYAPGETYPDTPELREYREKWLTREIASPP"
     gene            complement(1778..3253)
                     /locus_tag="VEL74_19040"
     CDS             complement(1778..3253)
                     /locus_tag="VEL74_19040"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG64684.1"
                     /translation="MKRLAVISAGLWVLVAVVVPPPLGGGAVGSGGGQEGGSVSLLPI
                     PEPDTSLMEPAVREQLEAERQAFAGASNLADAYGRRGRAFLVYELADAARACFENATR
                     LAPSDPRWPYYLGVIHQKKGDFAAAAASFARTLELRPQDAPALLRLGEVDLQRGDLAA
                     ARRRFEAALPLPGAAAAAHFGLGRVALGQDDATTAATAARHLETALGLQPGSSVVRYQ
                     LALAYRKLGQTGKARQQLSGYDHGGDARVRFADPLMSEVAALNAGARGHVVLATSAFR
                     AGRFAEAAAEYRKALEGNPDDPLVWGDLGTTLERLGDAAGAEQSYRRALELAPEDGRA
                     RQSLGTLLASRGRAREGIEHLEAAVRLLPGSRDARFNLARALAETGEPTKALEQYDQV
                     LALAPQDLGARYQKGMMLLALGRPGDAVAELDRVAADDPAAVAPRAAAAAALAAAGRF
                     PEAAERQRQAVDLATRAGHPGVEGLRECLVMYEAGKVCEGR"
     gene            complement(3250..4968)
                     /locus_tag="VEL74_19045"
     CDS             complement(3250..4968)
                     /locus_tag="VEL74_19045"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HYG64685.1"
                     /translation="MTRHRILPLLCLALSLGPVPGRSQSSLPFAERAREVGIDFTHVN
                     GMAGGWYMPEIMGAGAALFDFDDDGDLDAFLVQGGPFGPKAERSGAPGHRLYRNELIS
                     PSGRGPLRFTDVTAASGITLGDYGMGVAAGDFDNDGRTDLYVTSLGSNQLWRNNGPGK
                     GPDATGQVTFTDVTRQAGADDPRWSVPAAFVDFDRDGWLDLYVGTYLDFSFATYKPCR
                     TAAGAPDYCGPVPSDALPDRLLRNRRDGTFEDVSQKSGLAAARGPALGVTTADFDGDG
                     WTDLYVANDETPNHLWINQKDGTFRDDALLAGCAVSGEGRPQASMGIDTGDYDGDGDE
                     DLVMDNLTGEGITLYRSDGAGNFEDVSRASGLQAASWPFTGFGAAWLDYDNDSRLDLL
                     VVNGAVKVIEELRGKEPLPLRQTRQLFHNLGNVRFEDVSRQAGPVFNTPEVGRGAAFG
                     DVDNDGDTDVLVSNNNGPARLLINEAAAGRHWLGLRLVTGKRDALGARVTLERPGAPP
                     LVRRARADGSYASANDPRVLFGLGDSAKIGRVVVRWPSGKVEAFQGIAIDTYTTLREG
                     EGTPVP"
     gene            complement(4965..6950)
                     /locus_tag="VEL74_19050"
     CDS             complement(4965..6950)
                     /locus_tag="VEL74_19050"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HYG64686.1"
                     /translation="MRHPSMLWAVLTVAAVSVAGSPAPAQHAHGGAAARTLPPAAAQE
                     AGADGKVFHVPSQDVGAETLAARAAGQRSNAGKLKVFHDFGFTDRLPESGITFVHGIV
                     DDAGRRYKAAHYDHGNGIVAADVDGDGRTDLYFTSQRGGNELWKNEGGGRFRNVTAEA
                     GVGLAGRVSVSAAFGDVDNDGDPDLYVTTVRMGNVLFENDGKGRCKDVTGDSGLGYSG
                     HSSSAVFLDYDRDGWLDLFLVNVGKYTTEAHGEGGYYIAYEDAFRGHLFPERTEKSVL
                     YRNLGGRRFVDVSAHTGLQDGSWSGDATLTDFNEDGYPDLYVLNMQGDNHYYENQGGK
                     RFVDKTAQLFPKTPWGAMGVKSFDADNDGDLDLFITDMHSDMVQEVAPEDEKIKFVFK
                     GAERFFAEPENNIFGNAFYRNEGNGTFREASDQIWLENYWPWGVSVDDLNADGWDDIL
                     ITASMNFPYRYGINSLMLNNQGKEFLDSEFLLGIEPRRGGLTRKPWFTLDCSGADRPH
                     SLCANQTGRITVTGTLGTRSSVIFDLDGDGDLDIVTNELHSEPQVFVSDLARKRPVRH
                     LQVRLRGRETNRDGLGALVRVKVGERTLTKRMDGASGYLSHSLLPLYFGLGDAAAADR
                     VEVLWPTGRTQVVAGPIAAGRTLEIVEEVPAKGRTGS"
     gene            complement(6947..8014)
                     /locus_tag="VEL74_19055"
     CDS             complement(6947..8014)
                     /locus_tag="VEL74_19055"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYG64687.1"
                     /translation="MNGIRHIRPVSPAAASGLVARVYDQARRDFNVAPPIVLHSPVPD
                     LLAGMWCVLRESVIAGRVPRALKEALAAGVSRLNRCPFCIDAHTLMLAAAGQQAAAAA
                     IAAGREEEVEDDATRRLLAWAAAHRSPGSPELAPPFPPPLSSDNTAEMIGTALAFHYI
                     NRAAHVFLPETPYPLQVGNGWAKGLLRRGASRVFARSVTRSYTPGDSLALLPDVELPP
                     DLPWAAADPVIAGTYARFAASVDAAVQGALPDAVRDLVVARLTAWDGADPGLGMGWLE
                     EAVADLSKEDRPAGRLALLTAFASYRVDDSTVAAYRTRYPEDRQLVGAACWASYVAAR
                     QVGLWLWEAAYTPHHPLGDAG"
     gene            8138..9205
                     /locus_tag="VEL74_19060"
     CDS             8138..9205
                     /locus_tag="VEL74_19060"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HYG64688.1"
                     /translation="MPIGAISELASGRGPGAGLREPVLVTGATGFTGGHLARRLRQLG
                     HPVRALVRAGADPKPLLAQGIEVVEGDITRAADVERAAAGVCKIFHIAAVFRTAGHPD
                     SYYYAVNLGGTQNVLAAARRHGVERVLHCSTVGVHGGVHDIPSTEESPFNPGDIYQRT
                     KLAGEQAAREACAWGLPVTVVRPSPIYGPGDLRLLKLFRTVENGTFRMFGSGEAFFHL
                     VYIDDLIDGMLLCAERPEALGQVFILAGPRYLRLNEIVELIAGAVGVPPPRGRLPMWP
                     LMAAAAACEAICRPLHVEPPLHRRRADFFRKNRAFSTEKARCLLGYEPRVDPQDGIHR
                     TAQWYFENGHLGGAHRRRAAS"
     gene            9227..>9984
                     /locus_tag="VEL74_19065"
     CDS             9227..>9984
                     /locus_tag="VEL74_19065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HYG64689.1"
                     /translation="MSMTAVQEKKRPLRPKKLIELALRRTPPLYRLASRVYHRMNPSF
                     RTLSPGAPGAIRRAFELVRDRSPDGLVGDYYEFGLFRGFTFLSAQRSCDELGLAGTRF
                     WGFDSFQGLPAVEGIDRAEGEFFEGQFACGKQEVIDNLSRHGMDWSRAELIEGFFSDS
                     LTEELKRRLPFRPAGVVVLDCDLYSSTQEALSWMSGLLGATTVLLFDDWLSFGGTPDR
                     GQPLAFREFLERHPGLHSEEGWDFANNGRTFVVTR"
BASE COUNT         1377 a         3485 c         3664 g         1458 t
ORIGIN      
        1 gcggcctgac ccaggggaac ggcaagaaca accgcgacgg gctcggcgcc accctggagc
       61 tgcgcgccgg cagcgcctat cagttccgcg aggccgccgg ccaggtcacc cacttcggcc
      121 tcgggcagcg gcgccaggcc gacctgctgc gggtggtgtg gaccaacggc gtgccgcaga
      181 accgcattca gcccagcggg gagcagcggg tggtggagga gcaggtgctc aagggaagct
      241 gccccttcct ttatacatgg gacggcgagc gcttcgcctt cgtcaccgat ctcctgtgga
      301 acgcacccat cggcctcccc gtcgcgccgg gcaagtgggt gccccccgat cccgaggagc
      361 tggtgcgggt ggacggcgcg cgcccggacg gcggcgtcta taagctccgc atcaccgagg
      421 agctgtggga ggctgccttc ttcgaccaca cgcgcctctg ggtggtggac caccccgccg
      481 gggtggaggt ggcgagcagc ctacgggtga cccccggggg gccgcccgac ggacccgaca
      541 gagtcgtggc gagccgcggc ctgcgtcccg tggtctccgc gctggacggc acgggggccg
      601 acatgaccga gcgggtccgc gagcgtgacg aggtctatgc cggcggctac ccggagagct
      661 cctatcaggg gatgagcggg gagccctggg ccttcacctt cgatctaggg gaggcgccgg
      721 gccgtccgct ccgcctccac ctcgacggct ggatcttccc cgccgacgcg agcctcaacc
      781 tcgccgccgc ccagcgcccg gacctcgcgt acgtcccgcc gcggctggag gtggagaccg
      841 cggacggctg gcaggtgctg gtccccgcca tgggccaccc ggcgggcaag accaagaccc
      901 tggtggtgga cacgccgccg ctgccggccg gcgtgagccg tctgcgcatc gtcaccactc
      961 tctggctcgc ctgggatcgc gtcgcctggt cccccgagcc cgttgattcg gaaccggtgg
     1021 tgcgcgcccg cctcctgccg tccgaagccg acctccgcta ccgcggcttc tccgagctgg
     1081 tgcgccgctc ccccaacggc ccgcacggct acgactacag ccgcgtggca tctgaatctc
     1141 cctggctgcc cttccccggc cgctacacgc gctacggcga ggttcgcgag ctgctggagt
     1201 cgccggacga ccgcaccgtc gtcctcgccc cgggtgacga gatcgccctc accttcgacg
     1261 cgtccgctct gccacccctg caggagggtt ggacgcgcac ggttttcttg gagagccacg
     1321 gctgggacaa ggacgcggac cgcaacacct gggaggcgcg gcagatggag ccgctcccct
     1381 tccgcgggat gagcggctac ccctacgccc ccggggagac ctaccccgac acgcccgagc
     1441 tgcgcgagta ccgggagaag tggttgacgc gggagatcgc ctcccctccc tagcacagcg
     1501 ttcccaaaga acggcaaagc ttcgcccatg accggcacgg ctgtgccacc agccggcaca
     1561 gccgtgccga ccgccggctc ggccatgccg acgacgggcc cggccaagcc gacgacaggc
     1621 acggccatgc cggcggccgg catcgctcta ccggcggcag gcagcgctgt gccgacggct
     1681 ggcatcgccg cgcccatgac aggcaaggcc atgccgatga caggcacggc ttcgccgacg
     1741 gctggcacga ctcagtcggc tacgtgcgag gacgccctca cctcccctcg cacaccttcc
     1801 ccgcttcgta catcaccaga cactcccgca acccctccac gccgggatgc cctgcccgcg
     1861 tggcgaggtc cacggcctgg cgctggcgct cggcggcttc ggggaagcgg ccggcggccg
     1921 cgagggcggc ggccgcggcg gcgcgcggcg ccacggctgc gggatcgtcg gcggcgacac
     1981 ggtccaactc ggcgacggcg tcgccgggac ggccgagggc caggagcatc atccctttct
     2041 gatagcgggc gccgaggtcc tggggagcga gggcgagcac ctggtcgtac tgctccaggg
     2101 ctttcgtggg ctcgccggtc tctgccagcg cccgggcgag gttgaagcgg gcgtcgcggg
     2161 agccggggag gaggcggacg gcggcttcca ggtgctcgat cccctcgcgg gcccggccgc
     2221 gcgaggcgag gagggtgccg aggctctggc gcgcccggcc gtcttcgggc gccagctcca
     2281 gcgcccggcg atagctctgc tcggctccgg cggcgtcgcc gaggcgctcc agtgtggtgc
     2341 cgagatcccc ccagacgagc gggtcgtccg gattcccctc cagggccttg cggtactccg
     2401 cggcggcctc ggcgaagcgg ccggcccgga aggcgctggt ggcgaggacg acgtgaccgc
     2461 gggcgccggc gttgagagcc gccacctccg acatcagcgg atcggcgaag cggaccctcg
     2521 cgtcgccgcc gtgatcgtag ccggagagct gctgccgcgc tttgccggtc tgcccgagct
     2581 tgcggtaggc cagggcgagc tgatagcgga ccaccgagga cccgggctgg aggcccagcg
     2641 ccgtctcgag gtggcgcgcc gcggtggccg cggtcgtggc gtcgtcctgg ccgagggcga
     2701 cacggcccag gccgaagtga gcggcggccg cggctccggg gagggggagg gcggcctcga
     2761 agcggcgccg ggcggccgcg aggtcgccgc gctggagatc cacctcgccc aggcggagga
     2821 gcgccggcgc gtcctggggg cgcagctcga gggtccgggc gaagctcgcg gcggcggcgg
     2881 cgaagtcccc cttcttctgg tggatcaccc cgaggtagta gggccagcgg gggtcggagg
     2941 gggcgagccg ggtggcgttc tcgaagcagg cccgggcggc gtcggccagc tcgtagacga
     3001 ggaaggcccg gccgcggcgg ccgtaggcgt cggcgaggtt ggaggcgccg gcgaaggctt
     3061 ggcgctccgc ttcgagctgc tcgcggacgg cgggctccat cagggaggtg tcgggctcgg
     3121 ggatggggag gagagaaacg ctgcccccct cctgacctcc cccactccct accgcgcccc
     3181 ctcccagggg gggaggcacc accaccgcta ccagcaccca aagtccagcc gagatgacag
     3241 ccaggcgctt cacggcacgg gcgttccttc cccttcccgc agcgtcgtgt aggtgtcgat
     3301 ggcgatcccc tggaaagctt cgaccttccc gctcggccag cggacgacga cgcggccgat
     3361 cttggcgctg tcgcccaatc cgaagagaac ccttggatcg ttggccgagg cgtagctgcc
     3421 gtcggccctt gcccggcgga cgaggggcgg cgcgccgggg cgctccagcg tcacccgggc
     3481 tcccagggcg tcgcgcttgc cggtgacgag gcgcaggccg agccagtggc ggcccgcggc
     3541 ggcctcgttg atcagcaggc gcgccggacc gttgttgttc gagaccagga cgtcggtgtc
     3601 gccgtcgttg tccacgtctc cgaaggcggc gccgcggccg acctcggggg tgttgaagac
     3661 ggggccggcc tggcggctca cgtcctcgaa gcggacgttc ccgaggttgt ggaagagctg
     3721 ccgggtctgg cgcagcggca gcggctcctt cccccttagc tcctcgatga ccttgacggc
     3781 gccgttgacc accagcaggt cgagccgcga gtcgttgtcg tagtcgagcc aggcggcgcc
     3841 gaagccggtg aagggccagg aggccgcctg caggccgctg gcgcgggaga cgtcctcgaa
     3901 gttgccggcg ccgtcgctgc ggtagagggt gatcccctcg cccgtgaggt tgtccatcac
     3961 caggtcttcg tcgccgtcgc cgtcgtagtc gccggtgtcg atccccatgc tggcctgggg
     4021 acgcccctcg ccgctcaccg cgcagccggc gagcagggcg tcgtcccgga aggtcccgtc
     4081 cttctggttg atccagaggt ggttgggggt ctcgtcgttg gccacgtaga ggtcggtcca
     4141 gccgtcgccg tcgaagtcgg cggtggtcac cccgagggcg gggccgcggg cggcggcgag
     4201 gccggatttc tgcgacacgt cctcgaaggt gccgtcccgc cggttgcgca gcagccggtc
     4261 ggggagggcg tcggacggta ccggcccgca gtagtcggga gcgccggccg cggtgcggca
     4321 gggcttgtag gtggcgaagg agaaatcgag gtaggtgccg acgtagaggt cgagccagcc
     4381 atcgcggtcg aagtcgacga aggccgcggg gacgctccag cgcggatcgt cagccccggc
     4441 ctgccgcgtc acgtcggtga acgtcacctg ccccgtggcg tccgggccct tgccgggacc
     4501 gttgttgcgc cagagctggt tcgatccgag cgacgtgacg tagaggtcgg tccggccgtc
     4561 gttgtcgaaa tcgccggccg ccacccccat gccgtagtcc cccagggtga tccccgacgc
     4621 ggcggtgacg tcggtgaagc ggagcggccc tctaccggag ggggagatca gctcgttgcg
     4681 gtagagccgg tggccagggg ctcccgaccg ctcggccttg ggcccgaacg gccccccctg
     4741 caccaggaag gcgtcgaggt cgccgtcgtc gtcgaaatcg aagagcgccg cgccggcgcc
     4801 catgatctcg ggcatgtacc agccgcccgc catgccgttg acatgggtga agtcgatccc
     4861 cacctccctg gcgcgttcgg cgaaggggag agacgattga ctccggccgg ggaccggtcc
     4921 cagggacagc gcgaggcaga gcagggggag gatacggtgg cgcgtcacga tccggtacgg
     4981 cccttggcgg ggacctcctc gacgatctcc agcgtcctgc ccgcggcgat gggaccggcg
     5041 acgacctgcg tccgtcccgt cggccagagc acctcgacgc ggtcggccgc ggcggcgtcc
     5101 ccgagcccga agtagagggg gaggaggctg tgcgacaggt agcccgaggc gccgtccatc
     5161 cgcttggtga gcgtccgctc tccgaccttg acccgcacca gggcgccgag gccgtcgcgg
     5221 ttcgtctccc ggccgcgcag ccgcacctgg agatggcgga ccggccgctt ccgggccagg
     5281 tcgctgacga agacctgggg ctccgagtgc agctcgttgg tgacgatgtc gaggtcaccg
     5341 tcgccgtcga ggtcgaagat caccgaggag cgggtgccga gtgtgccggt gacggtgatg
     5401 cggccggtct ggttggcgca cagggagtgc ggccggtcgg cgccggagca gtcgagggtg
     5461 aaccagggct tgcgggtgag gccgccgcgg cgcggctcga tgccgagcag gaactcgctg
     5521 tcgaggaact ccttcccctg attgttgagc atcagcgagt tgatgccgta acggtagggg
     5581 aagttcatac tggcggtgat gaggatgtcg tcccagccgt cggcgttgag gtcgtcgacg
     5641 ctcacccccc agggccaata gttctccagc cagatctggt ccgaggcctc gcggaaggtg
     5701 ccgttcccct cgttccggta gaaggcgttg ccgaagatgt tgttctccgg ctcggcgaag
     5761 aaccgctcgg cccccttgaa gacgaacttg atcttctcgt cctcgggggc gacctcctgg
     5821 accatgtccg agtgcatgtc ggtgatgaag aggtcgaggt cgccgtcgtt gtcggcgtcg
     5881 aacgacttca cgcccatggc gccccagggg gtcttgggga agagctgcgc ggttttgtcg
     5941 acgaagcgct tgcccccctg gttctcgtag taatggttgt ccccctgcat gttgaggacg
     6001 tagaggtcgg gatagccgtc ctcgttgaag tcggtgagcg tggcgtcgcc gctccacgag
     6061 ccgtcctgga ggccggtgtg ggcggagacg tcgacgaagc ggcggccgcc gaggttccgg
     6121 tagaggacgc tcttctcggt gcgctccggg aacaggtggc cgcggaaggc gtcctcgtag
     6181 gcgatgtagt agccaccctc gccgtgcgcc tcggtggtgt acttgccgac gttgaccagg
     6241 aagaggtcga gccagccgtc gcggtcgtag tcgaggaaga ccgcggacga cgagtggccg
     6301 gagtacccga gccccgaatc gccggtgacg tccttgcacc gccccttgcc gtcgttctcg
     6361 aacaggacgt tgcccatgcg gaccgtggtc acgtagaggt cagggtcgcc gtcgttgtcg
     6421 acgtcgccga acgccgccga gaccgacacc cggccggcga ggccgacgcc cgcctcggcc
     6481 gtgacgttgc ggaagcgccc gcccccctcg ttcttccaca gctcgttgcc gccgcgctgg
     6541 ctggtgaagt agaggtcggt ccgcccgtcg ccgtccacgt cggcggcgac gatgccgttg
     6601 ccgtggtcgt agtgggccgc cttgtagcgc cggccggcgt cgtcgacgat gccgtggacg
     6661 aaggtgatgc cgctctccgg cagccggtcg gtgaacccga agtcatggaa gaccttgagc
     6721 ttcccggcgt tggaccgctg ccccgcggcg cgcgccgcca gcgtctcggc tccgacatcc
     6781 tgcgatggca catggaagac cttgccgtcg gccccggctt cttgagcggc ggcgggcggc
     6841 agcgtccggg cggccgcgcc gccatgggcg tgctgggcgg gggccggaga gccggccacc
     6901 gaaacggcgg cgacggtcag gacggcccag agcatcgagg ggtgtctcat ccggcgtctc
     6961 ccagagggtg gtggggagta tacgccgcct cccacaacca caatcccacc tgccgcgccg
     7021 ccacgtagct cgcccagcag gccgccccca ccagttgccg gtcctcggga tagcgggtcc
     7081 ggtaggcggc gaccgtcgag tcatccaccc ggtacgaggc gaaggcggtg agcagagcga
     7141 ggcggccggc cggcctgtcc tctttggaga ggtccgccac ggcctcctcc agccagccca
     7201 taccgaggcc gggatcggcg ccgtcccagg ccgtgagccg ggcgacgacc aggtcccgca
     7261 ccgcgtccgg cagcgctccc tggacggcgg cgtccactga ggcggcgaag cgggcgtagg
     7321 ttccggcgat caccggatcg gcggcggccc aggggagatc aggggggagc tcgacgtccg
     7381 gcagcagggc gagcgagtcg ccgggggtgt agcttcgggt gacgctgcgg gcgaagacgc
     7441 gggacgcgcc gcggcgcagg agccccttgg cccagccgtt gcccacctgc agggggtagg
     7501 gcgtctccgg caggaagacg tgggcggccc ggttgatgta atggaaggcc agggcggtgc
     7561 cgatcatctc ggcggtgttg tcggaggaaa gcgggggggg aaacggcggc gccagctccg
     7621 gcgagccggg ggaacggtgg gccgccgccc aggcgaggag gcggcgcgtg gcgtcgtcct
     7681 ccacctcctc ctcgcgcccg gcggcgatcg cggccgcggc ggcctgctgg ccggccgccg
     7741 ccagcatcag ggtatgagca tcgatgcaga aggggcagcg gttgaggcgc gacacccccg
     7801 ccgccagggc ctccttgagc gctcggggga ccctgccggc gatcaccgac tcccgcagca
     7861 cgcaccacat gccggcgagg agatccggta ccggcgagtg gaggacgatc ggcggcgcga
     7921 cgttgaaatc ccgccgcgcc tggtcgtaca cccgggccac cagccccgag gccgcggcgg
     7981 gtgagaccgg ccggatgtga cggatgccgt tcatgcgtcc tctccctctc ctctatctaa
     8041 tagtcggtgc cgcgcccgcc ctcttctacg ccgcgagggt atcaatcttg cttgagcgat
     8101 aggacgtcca gaagtagcta cggtttcaac tcgatctatg ccgataggcg ccatcagcga
     8161 acttgcctcg ggccgtggtc ccggcgctgg cctccgcgag cccgtcctgg tcacgggagc
     8221 gaccggcttc accggcggac acctggcgcg ccggctgcgg cagctcggcc acccggtgcg
     8281 ggccctggtg cgtgccggcg cggatccgaa gcccctgctt gcccagggga tcgaagtcgt
     8341 cgagggggac atcacccggg cggccgacgt cgagcgggcc gccgcgggcg tttgcaagat
     8401 cttccacatc gccgccgtgt tccgcaccgc cgggcacccg gactcgtact actacgcggt
     8461 caacctcggc ggcactcaga acgtcctggc ggcggcgcgc cggcacggcg tcgaacgggt
     8521 gctccactgc agcacggtgg gcgtccacgg cggcgtccac gatataccct ccaccgagga
     8581 gtcccctttc aaccccggcg acatctacca gcgcaccaag ctcgcaggcg aacaggctgc
     8641 ccgggaggcg tgcgcctggg ggctgcccgt caccgtggtc cggccgtctc ccatctacgg
     8701 ccccggcgac ctgcggctgc tcaagctctt ccgtacagtg gaaaacggca ccttccggat
     8761 gttcggttcc ggcgaggcct ttttccacct ggtctacatc gacgacctga tcgacgggat
     8821 gctgctctgt gccgagcggc cggaggcgct ggggcaggtg ttcatcctcg ccggtccgcg
     8881 ctacctgcgg ctcaatgaga tcgtggagct gatcgccggc gccgtcggcg tccccccgcc
     8941 gcgcgggcgt ctgcccatgt ggccgctgat ggcggcggcg gcggcgtgcg aggcgatctg
     9001 ccggccgctg cacgtcgagc cgccgctcca ccggcgccgc gccgacttct tccgcaagaa
     9061 ccgggcgttc tccaccgaga aagcccggtg ccttctgggc tacgagcccc gcgtcgaccc
     9121 gcaggacggc atccaccgga ccgcgcagtg gtacttcgag aacggccatc tgggcggggc
     9181 gcaccggcgc cgggccgcct cctgaagaga acggagacga tagacgatga gcatgacggc
     9241 agtccaggag aaaaagcggc cgttgcgtcc caagaagctg atcgagctgg cgctgcgccg
     9301 cacgccgccg ctctaccggc tggcctccag ggtctatcac cggatgaacc cctccttccg
     9361 gaccctcagc ccgggggcgc ccggcgccat ccgccgggcg ttcgagctgg tccgggaccg
     9421 ctcgcccgac ggcctggtgg gtgactacta cgagttcggc ctgttccgcg gcttcacctt
     9481 cctgagcgcg cagcggtcct gcgacgagct gggcctcgca ggcacccgct tctggggctt
     9541 cgactctttc caggggctgc cggccgtcga ggggatcgac cgggccgagg gggagttctt
     9601 cgaggggcag ttcgcctgcg gcaagcagga ggtcatcgac aacctcagcc gtcacggcat
     9661 ggactggtcg cgcgccgagc tgatcgaggg cttcttcagc gactcgctga cggaggagct
     9721 caagcgccgg ctgccgttcc ggccggccgg cgtggtggtc ctcgactgcg acctctactc
     9781 ctcgacgcag gaggcgctct cctggatgag cggcctgctg ggggccacca ccgtcctgct
     9841 cttcgacgac tggctgtctt tcggcggcac cccggaccgg gggcagcccc tggccttccg
     9901 cgagttcctc gagcggcacc ccggcctcca ctccgaggag ggctgggact tcgccaacaa
     9961 cggccgcacg ttcgtcgtca cccg
//
LOCUS       DASPJI010000672         3763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_154222, whole genome shotgun sequence.
ACCESSION   DASPJI010000672 DASPJI010000000
VERSION     DASPJI010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154222"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VEL74_19070"
     CDS             <1..718
                     /locus_tag="VEL74_19070"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HYG64690.1"
                     /translation="GLQVPDTPFLDTVEEMAAHYAAAMRGVQPHGPYLLGGWSIGGTV
                     AFELARQLAAAGEEVALLVLVDPPPPFQFGARQPDELEMISLFARDLAATQGRDLLVT
                     MSVAELRALAPAERLPRVLAEAEVAHLVPPGIGAAAAERLFHMYRTTREAARVYVPGP
                     YPGSAVVFLTTDPADPAGAAGDGAADDGSGWPELAAGGVEVEEIAGDHYSILRPPGVE
                     ALAARLAARLAPFDGRRSEP"
     gene            718..>3763
                     /locus_tag="VEL74_19075"
     CDS             718..>3763
                     /locus_tag="VEL74_19075"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64691.1"
                     /translation="MTTLQLLSHLRDLGVQLWAEDERLRFTAPQGALTGDLRNELAGR
                     KQEILRLLRQGQAGGLASTPVRMPDEVAAAGRFPLSFAQQRLWFLQQLDPASAAYNIS
                     RAMLLAGPVRVDLLERSLGEVVRRHAILRTTFAAAGGEPFQVIAPATGWRLPLVDLRD
                     LPAAARWAEMQRLSSLLGEEPMDLARGPLLRVVLLRLEAEEHVAAATLHHIISDAWST
                     GLLFREMGVVYGAFAQGMLSPLPELPIQYADYAWWQRRHLEGEVLERQIEYWMQQLAS
                     APEMLELPTDRPRSAARSSRGGRLGSLIPRELLEPLRTLGQEEGAATLFMVLLAGFDA
                     LLYRCSGQEDIVVGFPIANRSHTELEGLIGLFINTLAMRTRLAGGLSWRQLLVQVRQT
                     TLDAYAHQDLPFERIVEELRPERQLSQTPIFQVTFSLQNTPGSDMQVGPFRMSLLPPV
                     DKTAQFDLTCDIKETAEGAQISWRYREELFDEPTVARLSEHYLNLLAGVAAHPDRSLA
                     DLVLLSTAERHQLLAEWSEIPAPALPDLPLHALFEEQAARRPEAPAVEWDRGRLSYGE
                     LDLRAGRLARHLRGLGVRLEVPVAFCLERSPEAVVTILAILKAGGAYVPLDPAAPMER
                     LAWLLDDVGAPVLVTTVDLLERLPRTLAITVVLDELADLEGEGEEGDPEISVGADSLA
                     YLMYTSGSTGTPKGVAVLHRAVGRLVRCGDFAHFGPEQAWAQLAPLSFDASTLEIWGA
                     LLHGGRLVLMPELPDPAGLGEALARHRVTSLWLTAGLFHQMVEENPAGLAPLSQLLAG
                     GDVLSPEHVRRALAAHPGLRLVNGYGPTENTTFTCCGPLSEPGEVGWTVALGRPIGRT
                     RVHVLDASFQPVPIGVEGELYAGGNGLARGYHGRPDLTAERFVPDIWGTGDRLYRTGD
                     RARYLADGRLEFLGRRDRQVKIRGFRVEPREVEAALARHPGLREAVVEALSDATGGRR
                     LVAWVVPRGSEVVDTLDTGDLRRFLAETLPVYLLPAAFVVLPE"
BASE COUNT          476 a         1335 c         1369 g          583 t
ORIGIN      
        1 cggtctgcaa gtccccgaca ccccgttcct ggatacggtg gaggagatgg cggcccacta
       61 tgccgcggcc atgcgcggcg tccaaccgca cggcccttac ctcctgggcg gctggtccat
      121 cggcggcacc gtggcgttcg agctcgcccg ccagctcgcg gccgccggcg aggaggtggc
      181 gttgctcgta ctcgtcgacc ccccgccgcc gttccagttc ggcgcgcgtc agccggacga
      241 gctggagatg atctcgctgt tcgcgcgaga cctcgccgcc acccagggcc gtgacctcct
      301 ggtgaccatg tcggtcgccg agttgcgcgc cctggctccc gccgagcgcc tgccgcgggt
      361 gctcgccgag gccgaggtgg cccacttggt gccgccgggc atcggcgccg cggcggcaga
      421 gcgcctgttc cacatgtacc gcaccacccg ggaggcggcg agggtctatg tgccgggacc
      481 ctatcccggc agtgccgtgg tcttcctgac gaccgacccg gctgaccctg ccggcgctgc
      541 cggcgacggg gcggcggacg acggctccgg ttggccggag ctcgccgccg gcggcgtcga
      601 agtggaggaa atcgccggcg accattactc catcctgcgc ccgccgggcg tggaagcgct
      661 ggcggcgcgg ctggcggcgc ggctcgcgcc attcgacgga cggagatccg agccgtgatg
      721 acgacgttgc agctcctctc ccacctgcgg gacctgggag tccagctctg ggccgaggac
      781 gagcgcctgc gcttcaccgc gccccagggt gcgctgaccg gcgacctccg gaacgagctg
      841 gccgggcgca agcaggagat cctccgcctc ctgcggcagg ggcaggccgg cggccttgct
      901 tccacgccgg tccggatgcc ggatgaggta gcggcggcgg ggcgcttccc cctctccttc
      961 gcgcagcagc ggctctggtt cctccagcag ctcgacccgg cgagcgcggc ctacaacatc
     1021 tcgcgggcca tgctcctggc cggaccggta cgggtggacc tcctggagcg tagcctcggc
     1081 gaggtcgtcc gccggcacgc gatcctgcgc accaccttcg cggccgccgg gggcgagccc
     1141 ttccaggtca tcgcgcccgc cacgggctgg cgcctgccgc tggtggacct gcgggatctg
     1201 ccggcggcgg cgcggtgggc cgagatgcag aggctctcca gcctgctggg cgaggagccc
     1261 atggaccttg cccgcgggcc tctgctgcgg gtggtgctcc tgcgcctgga ggccgaagaa
     1321 cacgtcgcgg ccgcgaccct gcaccacatc attagcgacg cctggtccac tggcctgctc
     1381 ttccgcgaga tgggggtcgt ctacggcgcc tttgcccagg ggatgctgtc gccactcccc
     1441 gagttgccga tccagtacgc cgactacgcc tggtggcagc ggcggcacct ggaaggagag
     1501 gtgctggagc ggcagatcga gtactggatg cagcagctcg ccagcgctcc ggagatgctg
     1561 gagctgccca ccgaccggcc ccgttcggcg gcgcggtcga gccgcggcgg ccgtcttggc
     1621 tcactcatcc cgcgggagct gttggagccc ctcaggactc tcggccagga ggagggggcg
     1681 gcgaccctct tcatggtgct gctggccgga ttcgatgctc tcctctaccg ctgctcggga
     1741 caggaggaca tcgtcgtcgg cttccccatc gccaaccgca gccacacgga gctcgagggg
     1801 ctcatcggcc tgttcatcaa cacgttggcg atgcggacgc gcctcgccgg cggtctctcc
     1861 tggcgccagc tcctggtgca ggtgcggcag accacactgg acgcatacgc ccaccaggac
     1921 ctgcccttcg agagaatcgt ggaggagctg cggccggagc gtcagctcag ccagaccccc
     1981 atcttccagg tcacgttcag cctgcagaac acgccgggct cggacatgca ggtcggcccg
     2041 tttcgcatga gcctactgcc ccccgtggac aaaacggccc agttcgacct cacctgcgac
     2101 atcaaggaga cggcggaggg ggctcagata tcctggcgct accgggagga gctgttcgac
     2161 gagccgaccg tggcgcgcct gagcgagcac tacctgaacc tgctggccgg cgtggcggct
     2221 cacccggacc gctcgctggc ggatctcgtc ctgctctcca ccgccgagcg tcaccagctc
     2281 ctggccgagt ggtccgagat accggcaccg gcgctgccgg acctgccgct tcatgccctg
     2341 ttcgaggagc aggcggcgcg gcggcccgag gcgccggcgg tggagtggga cagaggacgg
     2401 ctctcctacg gcgagctcga cctccgggcc ggccggctgg cccgtcacct gcgtggcctg
     2461 ggagtgcggc tggaggtgcc ggtggcgttc tgtctcgagc gctctcccga ggcggtggtg
     2521 acgatcctcg ccatcctcaa ggccggcggc gcttatgtgc ctctcgatcc ggcagccccc
     2581 atggagcgcc tggcgtggtt gctcgacgac gtcggagcgc cggtcctggt gaccaccgtg
     2641 gacctgctgg aacgcctgcc gcggacgctg gccatcaccg tggtgctgga tgagctcgcg
     2701 gatctggaag gagaagggga ggagggcgat ccggagatct ccgtcggtgc cgacagcctg
     2761 gcctatctga tgtacacctc cggttccacc ggcaccccca aaggcgtggc ggtgctccac
     2821 cgcgcggtgg ggcggctggt gcgctgcggc gacttcgcgc acttcggccc ggagcaggcc
     2881 tgggcgcagc tcgcgccgct gtcgttcgac gcctcgacgc tggaaatctg gggggcgctg
     2941 cttcacggcg gccgtctggt gttgatgccg gagctgccgg acccggcggg gttgggggag
     3001 gcgctggcgc gccaccgggt gacctcgctg tggctcaccg ccggtctctt ccatcagatg
     3061 gtcgaggaga atccggccgg gctcgcgccg ctgtcccagc tcctcgccgg cggcgacgtg
     3121 ttgtcgcccg agcacgtgcg gcgggcgctg gccgcccatc ccgggctgcg cctggtcaac
     3181 ggctacggcc cgaccgagaa cactaccttc acctgctgcg gtccgctgtc ggagcctggg
     3241 gaggtgggat ggaccgtggc gctgggccgg cccatcggac gcacccgtgt gcacgtgctg
     3301 gacgcctcct tccagccggt acccatcggc gtcgaagggg agctgtacgc cggcggcaac
     3361 ggcttggccc ggggctatca cggccggccc gacctgacgg ccgagcgctt cgtgccggac
     3421 atctggggga cgggagaccg tctctaccgg acgggcgacc gggcgcggta tctggcggac
     3481 ggccggctgg aattcctcgg tcgccgggac cggcaggtca agattcgggg attccgcgtc
     3541 gagccgcggg aggtcgaagc ggcgctggcg cgccatccgg ggctgcggga ggcggtggtc
     3601 gaggccctgt ccgacgccac cggcggcaga cggctggtcg cctgggtggt gccgcgcggc
     3661 tcggaggtgg tggacaccct ggacaccggc gatctgcggc gcttcctggc cgagaccttg
     3721 cccgtctatc tgctgcccgc cgccttcgtc gtcctgccgg agc
//
LOCUS       DASPJI010000673         5323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_556829, whole genome shotgun sequence.
ACCESSION   DASPJI010000673 DASPJI010000000
VERSION     DASPJI010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556829"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VEL74_19080"
     CDS             <1..647
                     /locus_tag="VEL74_19080"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64692.1"
                     /translation="IDMAADPRAPGALWVAGGIGFFHSSDSGANWQPVNLGSEYLVID
                     VVKVSPRDPDVIFAAGMAVLDARPPRFEARIFRSVDGGRTWQRRDAGLPGQDIQELAL
                     DPEDPATLWASTSLDLYRSTDGGASWRRVRSALPDGVFTHLAAAPGAVYATLQGMGND
                     PSAVLRSTDGGETWTPLQTGLGFRIPSVLEIDPHDPDHLLLGTRGGSLLTWTEP"
     gene            complement(657..1673)
                     /gene="rtcA"
                     /locus_tag="VEL74_19085"
     CDS             complement(657..1673)
                     /gene="rtcA"
                     /locus_tag="VEL74_19085"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HYG64693.1"
                     /translation="MLELDGSHGEGGGQVVRTSLALSIVTGKPFRIVNLRARRSPPGL
                     RRQHLVAVEAAARVSGARVEGAVLGSRELTFVPGEAVPGEYDFPIGTAGSTTLVFQTV
                     LPPLLCAAGPSILTFEGGTHNPMAPPFEFLDRAFLPLVRRLGPKAEAELERPGFYPAG
                     GGRFTVTLEPVPKLQGFDLLERGPVRRRRATALVSRLPRHIAERELEQVARETGWEPA
                     ALEAREVDAFGPGNAVLLEVESENVTEVFNGIGERGLPAETVASRAVREMRRYLDAGV
                     PVGEHLADQLLLLLALAGSGAFATHPLSGHAATQAELIARFLDVRILVERTSEERCVV
                     RVES"
     gene            complement(1683..4091)
                     /locus_tag="VEL74_19090"
     CDS             complement(1683..4091)
                     /locus_tag="VEL74_19090"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HYG64694.1"
                     /translation="MRQSRNVLLWVLAAAAGLPLFMWAYPRAFPFTPGSWSITRYEAG
                     GIALERLRDLGDPVEDPYVVTSLATNPFFERRLQLALREGVRREQVQATGFPDLVAYW
                     QVAVYSADALRDDWTYLAEVALDGEVKSLRLRLDPRAPGAPISEVEARNRADAFLASQ
                     GIDLSLYGEPQLRSTQLTARTDLAVRYPDRRGGLGKDVSFGVEVLFAGDRLAGFRPWM
                     DDPQAREIQRALRPLQVTRYAWVMGVIVLVLVLALPFLRRYHAGEIGVRRAVQIFVLV
                     GGGGLLTMLLVARASSQDLGLGVVSRAQTTWLYALFGLMFNVIPVALLGFFTWAVGES
                     ICRERWGRKLAAFDALFQRQWRNSTVARSSLAGWSAGLLMSGLLAALLVLLQRWDVWP
                     LIAGTVGLQSGWPGLELLADSVCESLPFFMLVTICILPWAARWLGRVLGVVAAVLVGA
                     LVIFPPTLPLPLGPGVPVLLFVPAAVVAVFLIVDLLAALTAGAVMTALFAGYYLLTAD
                     DPALQVQGALALAALSAPLLASLRHLASDQEFVYRYEDVPPHVRRIAERERQRVELET
                     ARRIQSSILPELPPRIAGVDIAHAYLPASEVGGDFYDVLALEDGRLAVAVGDVAGHGV
                     SSGLVMSMAKSALAVQVTFNPEVAAVFRTLNRTIYQTARKRLLATLCYALLDPKQREL
                     LYASAGHLYPYRITAAGKVEALESTAYPLGVRGELQVDPRVAKLEPGDTLFLFSDGLV
                     EARPEGSEEMFGFERLERSLSSHAGESVEQLRDGILADIARFTGQAPREDDQTVLVLR
                     LPAA"
     gene            complement(4184..5113)
                     /gene="mdh"
                     /locus_tag="VEL74_19095"
     CDS             complement(4184..5113)
                     /gene="mdh"
                     /locus_tag="VEL74_19095"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HYG64695.1"
                     /translation="MRYKVTVVGAGNVGATTAQRIAEAELADVVMVDVAADLATGKAL
                     DMMEAAPVLGFNAKISGGGDYAPTAGSQICVVTAGLARKPGMSRDDLLNKNAEIIRGV
                     TEELVKYSPDTIIIMVTNPLDAMCEVARRVSGFPRERILGMAGVLDAARMRYFIASEL
                     GVSVEDVDAFVLGGHGDTMVPLPRYSTVNGIPLPQLIAADRIEAINDRTRNGGLEIVN
                     YYKQGSAYYAPSASAFEMVAAILHDKKKILPCAVLLEGEYGFKDLFIGVPARLGKGGM
                     EQVIELKLTTEEKAMLNKSAESVKSLVDALPKQ"
     gene            complement(5138..>5323)
                     /locus_tag="VEL74_19100"
     CDS             complement(5138..>5323)
                     /locus_tag="VEL74_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HYG64696.1"
                     /translation="LILSGCMMLRWLGWPEAASAIEEGLGRTIAQKRVTYDLERQMEG
                     ATLLKSSEFAEAIVENI"
BASE COUNT          840 a         1930 c         1847 g          706 t
ORIGIN      
        1 cgatcgacat ggccgcggac ccgcgcgcgc ccggcgctct ctgggtcgcc ggcggcatcg
       61 gctttttcca cagctccgac tccggcgcca actggcagcc ggtcaacctg gggagcgagt
      121 acctcgtgat cgacgtggtg aaggtgtcgc cgcgtgaccc cgacgtgatc ttcgccgccg
      181 ggatggccgt cctggacgcg cggccgccgc ggttcgaagc caggatcttc cgcagcgtcg
      241 acggcggccg gacctggcag cggcgcgacg ccggcctccc cgggcaggat attcaagagc
      301 tcgctctcga tcccgaggac cccgcgacgc tctgggcctc cacctccctg gatctttatc
      361 gcagcaccga cggcggcgcc tcgtggcggc gggtgcgctc ggcactgccg gacggcgtct
      421 tcacccacct cgccgcggcg ccgggagcgg tctacgccac cctgcagggg atgggcaacg
      481 atccttccgc cgtgctgcgc agcaccgacg gcggcgagac ctggaccccg ctccagaccg
      541 gcctgggttt ccgtatcccc agcgtcctgg agatcgatcc ccacgacccg gaccacctcc
      601 tcctcgggac ccggggcggc agcctgctga cctggaccga gccgtagagg ccgggctcag
      661 ctctccaccc gcaccacgca ccgttcctcc gacgtccgct ccaccaggat gcggacgtcg
      721 aggaacctgg cgatgagctc ggcctgggtg gcggcgtggc cgctcagggg gtgggtggcg
      781 aaggcgccgg agccggcgag cgcgaggagg agcaggagtt ggtccgccaa gtgctccccc
      841 accggcaccc cggcgtccag gtagcgccgc atctcccgga ccgcccggct cgccaccgtc
      901 tcggccggga ggccgcgctc gccgatgccg ttgaagacct cggtgacgtt ctcgctctcc
      961 acctccagca gcaccgcgtt gcccgggccg aaggcgtcca cctcccgggc ttccagcgcc
     1021 gcgggctccc aaccggtctc gcgcgccacc tgctccagct cccgctcggc gatgtggcgc
     1081 ggcagccgcg agaccagggc cgtggcccgc cggcggcgga ccgggccgcg ctccaggagg
     1141 tcgaacccct gcagcttcgg cacgggctcg agcgtcaccg tgaagcgccc gccgccggcc
     1201 ggatagaacc cgggacgctc cagctccgcc tcggccttcg gtcccagccg ccgcaccagg
     1261 gggaggaagg cgcggtcgag gaactcgaac ggcggcgcca tcgggttgtg ggtccccccc
     1321 tcgaaggtga ggatcgacgg gccggcggcg cagagcagcg gtggcagcac ggtctggaag
     1381 accagcgtgg tgctccccgc ggtgccgatc gggaagtcgt actcgccggg aacggcctcg
     1441 cccgggacga acgtcagctc ccgcgacccg agcacggccc cctccacccg cgcgccgctc
     1501 acccgcgccg ccgcctccac cgccaccagg tgctggcgcc gcagccccgg cggcgaccgg
     1561 cgggcgcgca gattgacgat ccggaacggc ttgccggtga cgatggagag cgcgagcgag
     1621 gtgcggacga cctggccgcc cccctcgccg tgtgatccgt ccagctccag catgggccga
     1681 tgctacgccg ccggcagccg caacaccaag accgtctggt cgtcctcgcg cggcgcctgg
     1741 ccggtgaagc gggcgatgtc ggcgaggatg ccgtcgcgca gctgctcgac gctctccccg
     1801 gcgtggctgg agaggctcct ctccaggcgc tcgaagccga acatctcctc gctcccctcg
     1861 ggccgcgcct ccaccaggcc gtcgctgaac aggaacagcg tgtcgcccgg ctccagcttc
     1921 gccacccgcg gatccacctg gagctcgccg cgcaccccca gcgggtaggc ggtggactcc
     1981 agggcctcca ccttgcccgc cgcggtgatc cggtaggggt agaggtggcc ggcgctggcg
     2041 tagaggagct cccgctgctt cgggtcgagc agcgcgtagc agagggtggc gagcagccgc
     2101 ttgcgggccg tctgatagat ggtgcggttg agcgtccgga agaccgccgc cacctccggg
     2161 ttgaaggtga cctgcaccgc cagggccgat ttggccatcg acatcaccag gcccgaggag
     2221 acgccgtggc cggcgacgtc gcccacggcc acggccaggc gcccgtcctc cagcgccagc
     2281 acgtcataga agtcgccccc cacctcggag gccggcaggt aggcgtgggc gatgtccacc
     2341 cccgcgatcc gcggcggcag ctccggcagg atcgagctct ggatgcgccg cgccgtctcc
     2401 agctccaccc gctggcgctc gcgctcggcg atgcgccgga cgtgcggcgg cacgtcctcg
     2461 tagcgataga cgaactcctg gtcgctcgcc aggtggcgga ggctggcgag gagcggcgcg
     2521 gacagggcgg cgagggccag cgccccctgg acctgaaggg cggggtcgtc ggcggtcagc
     2581 aggtaatagc cggcgaacag ggcggtcatc acggcgccgg cggtcaaggc cgccaagagg
     2641 tccacgatca ggaaaacggc caccaccgcg gccggtacga agagcagcac cggcacgccc
     2701 gggcccaggg gcagcggcag ggtcggcggg aagatcacca gcgcccccac cagcaccgcc
     2761 gccacgactc cgaggacacg cccgagccac cgcgccgccc agggcaggat acagatcgtc
     2821 accagcatga agaacggcag agactcgcag accgagtccg ccagcagctc cagccccggc
     2881 cacccgctct gcaggcccac cgtgccggcg ataagcggcc agacgtccca gcgttgcagc
     2941 agcacgagca gcgccgccag caggccggac atgagcagcc ccgccgacca gcccgcgaga
     3001 gacgagcgcg ccaccgtgga atttctccac tggcgctgga acagggcatc gaacgccgcc
     3061 agcttccggc cccaccgctc acgacagatc gactccccca ccgcccaggt gaaaaagccg
     3121 aggagcgcga ccgggataac attgaacatc agcccgaaca gcgcataaag ccaggtcgtc
     3181 tgggcccggg acacgactcc cagcccgaga tcctgcgacg acgcgcgggc gacgagcagc
     3241 atggtcagca ggccgccgcc gcccaccagc acgaagatct ggaccgcccg ccggacaccg
     3301 atctctcctg cgtggtaacg ccggaggaag ggcagggcga ggacgagcac caggacgatg
     3361 acccccatta cccaggcgta gcgcgtgact tgcagcggcc gcagggcgcg ctggatctca
     3421 cgcgcttgcg gatcgtccat ccaaggccgg aagccggcca gccggtcgcc ggcgaacagc
     3481 acctcgacgc cgaaggaaac atcctttccg agcccacccc gccgatccgg atagcgcacc
     3541 gcgaggtcgg tacgggcggt gagctgggtg ctgcggagct gcggctcacc gtagagagag
     3601 aggtcgatcc cctgggaggc caggaaggcg tctgcccggt tgcgcgcctc gacttcggag
     3661 atcggcgcgc ccggtgcccg cgggtcgagc cgcagacgca acgacttgac ctcgccgtcg
     3721 agcgcgacct cggcgagata agtccaatcg tcccggagcg cgtcggccga gtagacggcc
     3781 acctgccaat aagccaccag gtcgggaaaa cccgtggcct ggacctgctc ccggcgcacg
     3841 ccctcccgca aagcgagctg cagacgccgc tcgaagaacg ggttcgtcgc cagcgatgtg
     3901 acgacgtagg gatcctcgac cgggtcgccc aggtcgcgca accgctccag ggcgatcccc
     3961 ccggcctcgt agcgggtgat gctccagctc ccgggcgtga agggaaaggc ccggggatag
     4021 gcccacatga acaaaggcag cccggcggcc gccgcgagga cccagagcag gacgttgcgc
     4081 gactgtcgca tcgagagagt ttacgaaaga gggcggggtg gagtttccgt ttcgggcggc
     4141 gaagtgtggg cagggcgcgc cggacgcacc ctgcccgcga gcctcactgc ttgggcaggg
     4201 cgtccaccag gctcttcacc gactcggcgg acttgttgag catcgccttc tcctcggtgg
     4261 tcagcttgag ctcgatcacc tgctccatgc cccccttgcc gaggcgggcg ggcacgccga
     4321 tgaacaggtc cttgaagccg tactccccct cgagcagcac ggcgcagggg aggatcttct
     4381 tcttgtcgtg gaggatggcg gccaccatct cgaaggccga ggccgacggc gcatagtagg
     4441 ccgacccctg cttgtagtag ttgacgatct cgaggccgcc gttccgggtg cggtcgttga
     4501 tcgcctcgat gcggtcggcc gcgatgagct gcggcagggg gatgccgttg accgtcgagt
     4561 agcgcggcag cggcaccatg gtgtcgccgt ggccgccgag cacaaaggcg tcgacgtcct
     4621 cgaccgacac gccgagctcg ctggcgatga agtagcgcat gcgggcggcg tcgagcacgc
     4681 cggccatgcc gaggatccgc tcgcggggga agccggagac ccggcgcgcc acttcgcaca
     4741 tggcgtcgag cgggttggtc accatgatga tgatggtatc gggggagtac ttgaccagct
     4801 cctcggtgac gccgcggatg atctcggcgt tcttgttgag caggtcgtcg cggctcatgc
     4861 ccggcttcct ggcgaggccc gcggtgacga cgcagatctg cgaaccggcg gtcggagcgt
     4921 agtcgccgcc gccgctgatc ttggcgttga agccgaggac gggggcggcc tccatcatgt
     4981 cgagggcctt gccggtggcc agatcggccg ccacgtccac catcaccacg tcggccagct
     5041 cggcctccgc gatccgctga gcggtggtcg cgccgacgtt gccggcgccc accacggtaa
     5101 ctttatatcg catgggttga atctctcctg atcctagcta gatgttctcg acgatggctt
     5161 ccgcgaactc ggagctcttg aggagggtgg ccccctccat ctggcgctcc aggtcgtagg
     5221 tgacccgctt ctgggcgatg gtccgcccca gcccctcctc gatggcgctc gcggcctcgg
     5281 gccagccgag ccagcgcagc atcatgcagc cggagaggat gag
//
LOCUS       DASPJI010000674         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_849217, whole genome shotgun sequence.
ACCESSION   DASPJI010000674 DASPJI010000000
VERSION     DASPJI010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849217"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2432)
                     /locus_tag="VEL74_19105"
     CDS             complement(<1..2432)
                     /locus_tag="VEL74_19105"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYG64697.1"
                     /translation="MSSILVVEPEHRYVARISEALSGEGWKVRVVEGRDQALQAAASE
                     APDLVVVNAEIPGSDYLTTSFSRNVGGPGVLALLPENGGGQAAAGNITADELLAKPFS
                     DQDLLDTVRRGLAASAAAAAARRSPSPQRSTPEHKLTSQEIFADVLADLDEEPAAAPA
                     APPRAAAPAPAPAPPAPVPPTPRPVPAVPAATAGASAGDRDIERRLEKTLSGVLGAEP
                     RPRPAAPAQAPAPPRAQTPAGDVDALLSKTLGNLELGARPKPADAPRPAAPAAARPAA
                     PAPAQPAPPPSAPPARPTAPPVAASPAVSPQEGTAVRKAPQKTVGDFDFSELEELART
                     GRRPAQGTSPGTSPGTSPGTSPGTAVRPPAPVAQAPVQPAAPRPAPAPPPVAAAPPAA
                     PAPPPPLAPAAPVGARVNAVEDPGATQRIPIFPRQEQGNPGEHFGQYQLLERIAVGGM
                     AEVWKARMRGVEGFQKMVAIKKILSHMTDNAEFIGMFIDEAKLAAQLNHPNIVHIYDL
                     GKIGRDYYIAMEYVEGKDLRSLLNASRRKGMPLPMGLALLIAARLASALNYAHRKRDY
                     EGKELGLVHRDVSPQNVLITPDGDVKLCDFGIAKAVSKAGQTQIGSLKGKLQYMSPEQ
                     AWGRQVDPRSDIFSLGAVLFEMLTGERLFPGDSEMSVLEAVRQVRTRRAREVVPTIPQ
                     DVDDIVARALASEPADRFQNAGEMEQRLEAVLSGLIPAAPNHTDLSAYVRRVLAPQED
                     EDEIAIPEFHFSPEPRTVTPPPRPAVPESGAQVASPAQAAPQPTPPPAAPPVPQPLQA
                     PPPLQVPPAQATP"
     gene            2617..>3001
                     /locus_tag="VEL74_19110"
     CDS             2617..>3001
                     /locus_tag="VEL74_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYG64698.1"
                     /translation="MSGHSKWSTIKHKKAATDARRGKIFTKVLKEITVAARIGGGDPK
                     GNARLRSAIAEARSNSVPNDNIERAIKKGTGELEAEVYEELTYEGYGPAGVAVLAEVM
                     TDNRNRTAQDMRFVFARNGGNLGETG"
BASE COUNT          398 a         1035 c         1166 g          402 t
ORIGIN      
        1 ggggtggcct gcgccggcgg cacctgcagc ggcggcggcg cctggagcgg ttgcggcacc
       61 ggcggagccg cgggcggcgg cgtgggctgt ggcgcggcct gggcgggcga tgccacctga
      121 gccccgctct ccggcaccgc cggcctgggc ggcggggtga ccgtccgggg ctcgggtgag
      181 aagtggaact ccgggatggc gatctcgtcc tcgtcctcct gcggcgccag cacgcggcgg
      241 acataggcgg agaggtcggt gtggttcggc gccgccggga tcagcccgga gagcacggct
      301 tctagccgct gctccatctc gccggcgttc tggaagcggt ccgccggctc gctggccagc
      361 gcccgggcga cgatgtcgtc cacgtcctgc gggatcgtgg ggaccacctc gcgggcgcgg
      421 cgggtgcgca cctggcgcac ggcctcgagg accgacatct cgctatcgcc ggggaacagg
      481 cgctcgccgg tcagcatctc gaacagcacc gcgccgaggg agaagatgtc ggagcgcggg
      541 tccacctgcc ggccccaggc ctgctcgggg gacatgtact ggagcttccc cttgagcgag
      601 ccgatctggg tctggccggc cttggagacc gccttggcga tgccgaagtc gcacagcttg
      661 acgtcgccgt ccggcgtgat cagcacgttc tgcggcgaga cgtcgcggtg gacgaggccc
      721 agctccttgc cctcgtagtc gcgcttgcgg tgcgcgtagt tgagggcgct ggccaggcgg
      781 gcggcgatca gcagggcgag gcccatgggc agcggcatgc ccttgcggcg gctggcgttg
      841 agcagcgacc gcaggtcctt gccctcgacg tactccatcg cgatgtagta gtcgcggccg
      901 atcttgccca ggtcgtagat gtggacgatg ttcggatggt tgagctgggc cgccagcttc
      961 gcctcgtcga tgaacatgcc gatgaactcg gcgttgtccg tcatgtgcga gaggatcttc
     1021 ttgatggcga ccatcttctg gaagccttcg accccccgca tgcgggcctt ccagacctcg
     1081 gccatgccgc cgacggcgat ccgctccagg agctggtact ggccgaagtg ctcgcccggg
     1141 ttgccctgct cctgccgcgg gaagatcggg atgcgctgcg tcgcccccgg gtcctccacg
     1201 gcgttgaccc gggcacccac cggcgccgct ggcgccagcg gaggcggcgg agccggagcg
     1261 gccggcggag cggcggccac cggcggcggg gccggagcgg gccggggtgc cgcgggctgg
     1321 accggcgcct gggcgaccgg tgccggcggc cggacggccg tgcccggtga ggtacccggt
     1381 gaggtacccg gtgaggtacc cggcgaggtg ccctgggcgg gccggcggcc ggtacgggcg
     1441 agctcctcga gctccgagaa gtcgaagtcc cccaccgtct tctggggagc cttgcggacc
     1501 gcggtcccct cctgcggcga gacggcgggg gaggcggcca ccggcggtgc cgtgggccgg
     1561 gcggggggcg cgctcggggg aggcgccggc tgggctggcg caggggctgc cggacgagcc
     1621 gcggcgggtg ccgccggccg gggggcgtcc gccggcttcg gacgggcgcc gagctccagg
     1681 ttgccgaggg tcttgctgag cagggcgtcg acgtcgcccg ccggggtctg ggcccgcggc
     1741 ggcgccggag cctgcgccgg agcggcgggg cgcggccggg gctcggcccc gaggaccccg
     1801 gagagggtct tttccagccg ccgctcgatg tcccggtccc cggcgctcgc gccggctgtc
     1861 gcagccggca ccgccggcac ggggcgcggt gtcggaggaa cgggcgccgg aggagctggc
     1921 gccggagccg gggccgccgc ccggggcggc gcggccgggg cggcggccgg ctcctcgtcc
     1981 agatcggcga ggacgtcggc gaagatctcc tgcgaggtca gcttgtgctc cggggtgctc
     2041 cgctgggggg atggcgaccg gcgggccgcc gcggcggcgg ccgacgccgc cagcccgcgc
     2101 cgcaccgtgt cgagcaggtc ctggtcgctg aacggcttgg ccagcagctc gtcggccgtg
     2161 atgttgccgg cggcggcctg cccgccgccg ttctcgggca gcagggcgag gacccccggc
     2221 ccgccgacat tgcgggagaa ggaggtggtc agataatcgc tcccggggat ctccgcgttg
     2281 accaccacca ggtcgggggc ctcggaggcg gcggcctgga gcgcctgatc ccgcccctcg
     2341 accacgcgca ccttccagcc ctcgccgctc agcgcctcgc tgatccgggc cacgtagcga
     2401 tgttcaggct cgacgacgag gatcgaggac atacgggttc tccagcaaaa gagttacggc
     2461 aagcctttgt agcatccccg ccgccccccg tcaatccgaa aagggggaaa aacgctgatt
     2521 tttgagagat ttaacccccc gtctggcagg ggacggcccc catccattag agtaggtgcg
     2581 cgtccgccca tggaccggtc cccaggagca tgcacgatgt caggccatag caagtggagc
     2641 acgatcaagc acaagaaggc ggccaccgac gcgcgccgcg gcaagatctt caccaaggtg
     2701 ctcaaggaga tcaccgtcgc cgcccgcatc ggcggcggcg accccaaggg gaacgcgcgc
     2761 ctgcgcagcg ccatcgccga ggcgcggtcg aacagcgtcc ccaacgacaa catcgagcgc
     2821 gccatcaaga agggcaccgg cgagctcgag gccgaggtct acgaagagct cacctacgag
     2881 ggctacgggc cggccggcgt ggcggtcctg gccgaggtca tgaccgacaa ccgcaaccgc
     2941 acggcgcagg acatgcggtt cgtcttcgcc cgcaacggcg gcaacctcgg cgagaccggc
     3001 t
//
LOCUS       DASPJI010000675         5663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_340954, whole genome shotgun sequence.
ACCESSION   DASPJI010000675 DASPJI010000000
VERSION     DASPJI010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340954"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>5663
                     /locus_tag="VEL74_19115"
     CDS             <1..>5663
                     /locus_tag="VEL74_19115"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64699.1"
                     /translation="VLRVGESGEGPAEIPWLDWQREGLTPSALRRLLEKRTPEVLGLA
                     NIPNARVAAAAAAARLLAGGSEEIATAGELRQRAAAAAATAVDPQNFWELLRHLPYEV
                     ELGWAAPSGDGRFEAVLRRAGRGAAAPRLPLSALLPPPRNAAAPLAWSHYANNPMQGR
                     FARSMAPELRAFLAGRLPDYMVPSIFVPLDALPLSPTGKVDRRRLPAPELSRREGAAF
                     VAPRTPIERELARIFAELLGMERIGAGDNFFELGGHSLLATQVVSRAREAFRVEVPLR
                     ALFQAPTVAGLAERIEAERRAGTGLAAPPIVPVPRQGLENGLPLSFAQQRLWFIDQME
                     PGSSLYNVATALRMTGRLDLAALAGSFGEVARRHESLRTVFGEVEGVPVQQALPPAAV
                     LPPVVDLSGLDPGRRGGAALALAAAEAQRPFDLARGPLFRLGLLRLSGEEHVMLLTLH
                     HVISDGWSMGVLVREVAALYPALLAGGPSPLPELSVQYADFAVWQRHWLRGEVLEREL
                     AYWKGHLAGAPPVLALPADRPRPAVQSFRGALRPLWLPEPLMEELRALGLRRDATLFM
                     TLLAAFQTLLARYSGQWDVSVGTPIAGRNRHEIEGLIGFFVNTLVLRTDLSRDPGFDD
                     LLDRVREVSLAAQTHQEVPFEKLVEELEPVRSLSSAPLFQVMLALQNAPAGALELPGL
                     TLSPLPDGRTTAKFDLTLFLEEIAGGLGGALEYSRDLFEAPTAERMLGHFRALLQGVA
                     AAPAARLSELPLLSPAERQALLLEWNATGGPAPAGTWMERFRRQAARTPQAVAVAAPD
                     EELTYAALAARVRRLAGHLRSLGIGPGDRVGVWGERSVAAVVGLLGALEAGAAYVPLD
                     PSYPAERLAFLIQDSGIRALLARDRGDGLPPVAHLEAIEVIDPDAGDGLVAAADPASG
                     QAPEPADLAYVIYTSGSTGLPKGVMVSHGGLANYLDWAVEAYAAGGACSAPLHSSLGF
                     DLTVTSLFIPLLTGGTLHLLPEQEGVEALATALRRRSFPLLKITPAHLLVLSHLLADA
                     DLSAHRGTVVVGGEALLEEHVAWWWDRAPGLVVVNEYGPTETVVGCCVYTVPMSGAAG
                     PESGSVPIGRPIARTRLYVLDRDRRPLPSGVPGELFIGGAGVARGYLRRPDLTADRFI
                     PDSFAGAAGERLYRTGDLARYLPGGDLEYLGRTDQQVKIRGFRVEPGEVEAVLAGHPA
                     VRDCAVLARRDAQGASRLVAYVTGVAGEDLRPPELRSYLERRLPQYMVPSAFVAVAEL
                     PLTVHGKVDRLALPADPAERTDPAERTDPTDPVSELLAGIWAEVLGRDRIGVHDDFFA
                     LGGHSLLATQVVSRIRGVLGVDLSLRELFESPTVAKLAAAVRSARGQEVPPILTVPRE
                     GDLPLSFPQQRLWFLDQLEPGKAIFNQPSATRLTGKLSPSLLARVFTEIVRRHESLRT
                     TFESREGRPVQIVGPALPVELPVMDLAGLPPVEREAEARRLAAAEAGRPFVLRRGPVL
                     RLGLLRLAEQDHLLLMTLHHIASDAWSVALLVHEVAALYAAFSERRPSPLPELPLQYG
                     DFAVWQRNRLQGAVLEEQLAYWREKLVGAQTVLDLPTDRPRPALQAGHGASLIFELAG
                     APADAVLSLSRRCKVTPYMVSLAAFQIVLQRYAGQDDFLVGSTIAGRDRQELEALIGF
                     FVNTLLLRTGLRGDPSGEELLERVRETALGAFTHQELPFERLIEELKVERDLSRSPGF
                     QVVFAFQNVPLPRVSALPGLLMEPLAGGAPTAKYDLELTLAQVEGRLAGDLLYSTDLF
                     DAVTARRLLSQYRVVLAALAQRAQSPIGELPWLTPAEAHQLRTEWTEVLPAPLPASSI
                     PAAFEEWAERTPQALALVCSTEERTYGDLNREANRLALHLLRLGA"
BASE COUNT          664 a         2017 c         2113 g          869 t
ORIGIN      
        1 cgtgctgcgg gtgggggaga gcggcgaggg cccggccgag atcccctggc tcgattggca
       61 gcgggaaggg ctcaccccga gcgcgctgcg gcggctcctg gagaagcgga cgccggaggt
      121 cctggggctc gccaacatcc ccaacgcccg ggtcgccgcc gcggcggcgg cggcccgcct
      181 gctcgccggc ggatccgagg agatcgcgac cgccggcgag ctgcgccagc gcgccgcggc
      241 agccgcagcc acggccgtcg atccccagaa tttctgggag ctcctccgcc acctgcccta
      301 cgaggtcgag ctcggttggg ccgcgccgag cggggacggc cgcttcgaag cggtgctgcg
      361 gcgggccggc cgcggtgccg cggcaccgcg cctgcctctc tcggcactgc tgccgccgcc
      421 gcggaacgcg gcggcgccgc tggcctggag ccactacgcc aacaacccga tgcaggggcg
      481 cttcgcccgc agcatggcac ccgagctgcg cgccttcctg gccggccggc tccctgacta
      541 catggtgccg tcgatcttcg tgccgctcga cgcgctgccg ctttcgccga ccggcaaggt
      601 ggaccggcgc cggttgccgg cgcccgagct gtcgcggcgg gaaggggccg ccttcgtcgc
      661 accacgcacg ccgatcgagc gggagctggc gcggatcttc gccgagctcc tggggatgga
      721 gcggatcggg gcgggggaca acttcttcga actgggagga cattcgctcc tcgctacgca
      781 ggtagtgtcg cgggcacgcg aggcgttccg ggtcgaggtg ccgctgcgcg ccctgttcca
      841 ggcgccgact gtcgccgggc tggcggagcg gatcgaggcc gagcgacggg ccgggacggg
      901 gctggcggcg ccgccgatcg tgccggtgcc ccggcagggt ctcgagaacg gattgccgct
      961 ctccttcgcg cagcagcggc tctggttcat cgatcagatg gagccgggca gctcgctcta
     1021 caacgtcgcg accgcgctgc gaatgaccgg ccggctcgac ctcgccgccc tggccggcag
     1081 cttcggcgag gtcgcgcggc gccacgaatc cctgcgaacg gtcttcggcg aggtcgaggg
     1141 ggtgccggtg cagcaggccc tgcccccggc ggccgtcctc ccgccggtgg tcgacttgtc
     1201 ggggctcgac cctggccggc gggggggcgc ggcgctcgcg ctcgcggcgg cggaggcgca
     1261 gcgcccgttc gacctggcgc gcgggccgct cttccgcctc ggcctgctgc ggctgagcgg
     1321 tgaggagcac gtcatgctcc ttactctgca tcacgtgatc agcgacggct ggtcgatggg
     1381 tgtcctggtg cgcgaggtgg cggcgctcta cccggcgctc ctggccggcg ggccgtcgcc
     1441 gctgcccgag ctgtcggtcc agtacgccga cttcgcggtc tggcagcggc attggctgcg
     1501 gggcgaggtg ctggagcgcg agctggccta ctggaagggc catctggcgg gagcgccgcc
     1561 ggtcttggcg ctgccggccg accggccgcg gccggcggtg caaagcttcc gcggcgcgct
     1621 gcgccccctg tggctgccgg agccgctgat ggaggagctg cgggcgctcg gcctgcgccg
     1681 ggacgccacg ctgttcatga ccctcctcgc cgccttccag acgctgctcg cgcgctacag
     1741 cggccagtgg gacgtgagcg tggggacgcc gatcgcgggg cgcaaccgcc atgagatcga
     1801 agggctgatc ggcttcttcg tcaacaccct ggtgctgcgc accgatctgt cgagggatcc
     1861 cggcttcgac gatctgctcg accgggtgcg agaggtgtcg ctcgccgctc agacccatca
     1921 ggaggtcccc ttcgagaaac tggtagagga gctcgagccg gtgcgaagcc tctcctctgc
     1981 gccgctcttc caggtgatgc tcgcgctcca gaacgcgccg gccggcgcgc tcgagctgcc
     2041 gggcctcacc ctctccccgc tgccggacgg ccggacgacg gcgaagttcg atctgacgct
     2101 cttcctcgag gagatcgccg gagggctcgg cggcgcgctc gaatacagcc gcgacctgtt
     2161 cgaggcgccg acggccgagc gcatgctggg gcacttccgt gctctcctcc agggggtggc
     2221 cgccgcgccc gccgcgcggc tgtcggagct gccgctgctc tcgccggcgg agcggcaggc
     2281 gctgcttctc gaatggaacg cgaccggggg cccggcgccg gccggcacgt ggatggaacg
     2341 cttccgccgg caggcggcga ggacgcccca ggcggtggcg gtcgctgcgc cggacgagga
     2401 gctgacgtat gccgcgctcg ccgcgcgtgt tcgccgcctg gccggtcatc tgaggtctct
     2461 ggggatcggg ccgggcgacc gggtcggagt ctggggtgag cgctccgtcg cggccgtggt
     2521 cggcctgctc ggcgcgctcg aagcgggcgc cgcctatgtt cccctggatc ccagctaccc
     2581 ggccgagcgc ctcgccttcc tgatccagga ctccgggatc cgcgcgctgc tcgcccggga
     2641 ccgcggggac ggcctgccgc cggtcgccca cctcgaggct atcgaggtta tcgatccgga
     2701 cgccggcgac gggcttgtgg cggcggccga tcccgcgtcc ggtcaggccc cggaacccgc
     2761 ggacctcgcc tatgtgatct acacctcggg ctcgaccggc ctccccaaag gggtcatggt
     2821 ctcccatggc gggctcgcca actatctgga ctgggcggtc gaggcctatg ccgcgggcgg
     2881 ggcctgctcg gcgccgctcc attcctcgct cggcttcgat ctgacggtga ccagtctctt
     2941 catcccgctg ctcaccggcg ggacgctcca cctgctgccg gagcaggagg gcgtcgaggc
     3001 gctcgccacg gcgttgcgcc ggcggagctt cccgctgctc aagatcacgc ccgcgcatct
     3061 cctggtcctc tcccacctgc tcgcggacgc ggatctctcg gcccaccgcg ggaccgtcgt
     3121 cgtcgggggc gaggccctgc tcgaggagca tgtggcatgg tggtgggacc gggccccggg
     3181 cctggtggtg gtcaacgaat acggaccgac cgagacggtc gtcgggtgct gcgtctacac
     3241 ggtgccgatg tccggcgcgg ccggcccgga gtcggggagc gtgccgatcg gccgccctat
     3301 cgcccgcacc cggctctatg tgctggaccg cgaccgccgg ccgctccctt ccggcgtacc
     3361 gggggagctc ttcatcggcg gcgccggcgt ggcccgcgga tatctccgcc ggccggatct
     3421 gaccgccgac cgcttcatcc cggattcgtt cgcgggggcc gcgggagaga ggctctaccg
     3481 gacgggggac ctcgcgcggt atctgcccgg cggcgatctc gagtatctgg gacggaccga
     3541 ccagcaggtg aagatccgcg gcttccgcgt cgagccggga gaggtggagg cggtgctggc
     3601 gggtcacccc gcggtgcgcg actgcgcggt gctggcacgg cgcgacgccc agggcgcgtc
     3661 ccgtctcgtg gcttacgtca cgggcgtcgc gggcgaggac ctgcggccgc cggagctgcg
     3721 gagctacctg gagcggcggc tgccccagta catggtgcca tcggccttcg tggccgtcgc
     3781 ggaactgccg ctgaccgtgc acggcaaggt agaccggctg gcgctgccgg ccgatccggc
     3841 cgagcggacc gatccggccg agcggaccga tcccaccgat ccggtcagcg agctgctggc
     3901 gggaatctgg gccgaggtgc tgggccgcga ccggatcggc gtccacgacg acttcttcgc
     3961 tctgggcgga cattcgctcc tggcgacgca ggtggtgtcg cggatccgtg gagtcctcgg
     4021 ggtggacctg tcgttgcggg agctgttcga gtcccccacg gtcgcgaagc tggcggccgc
     4081 cgtgaggtcg gcaagggggc aggaggtgcc gccgatcctg acggtgccac gagaggggga
     4141 tctgccgctg tcctttcccc agcagcggct ctggttcctc gaccagctcg agccggggaa
     4201 ggcgatcttc aaccagccgt cggcgacacg cctgacaggg aagctgtcgc cgtcgcttct
     4261 ggcgcgggtc ttcaccgaga tcgtgcgccg gcacgagtcg ttgcgcacca ccttcgaatc
     4321 gcgcgagggg cggccggtgc agatcgtcgg cccagcgctg ccggtcgagc tgccggtgat
     4381 ggatctggcg ggtctgccgc cggtggagcg ggaggccgag gcgcgccggt tggccgcggc
     4441 cgaggccggg cggcccttcg ttctgcggcg tgggccggtc ctgcggctcg gtctcctgcg
     4501 cctggccgag caggaccacc tgctgctgat gaccctgcac cacatcgcct cggatgcttg
     4561 gtcggtggcg ctgctggtgc acgaggtggc ggcgctctat gcggcgttct cggaacggcg
     4621 cccgtcgccg ttgccggagc ttcccctcca gtacggcgac ttcgcggtct ggcagcgcaa
     4681 ccggctgcaa ggcgcggtgc tggaagagca actcgcctac tggcgggaga agctggttgg
     4741 agcacagacc gtcctcgacc tgccgaccga ccggccgcgg ccggcgctcc aggcggggca
     4801 tggggcgagc ctgatcttcg agctcgccgg cgcgccggct gatgcggtcc tgtcgctctc
     4861 ccggcggtgc aaggtgaccc cctacatggt ctcgctggcg gcgttccaga tcgtcctcca
     4921 gcgctacgcc ggccaggacg acttcctcgt cggctcgacg atcgccggcc gcgatcgcca
     4981 ggagctcgag gcgctgatcg ggttcttcgt caacaccctg ttgctccgca cggggctgcg
     5041 gggggaccct tccggggagg agctgctgga gcgggtccgg gagacggcgc tcggcgcctt
     5101 cactcatcag gagctcccct tcgagcgcct gatcgaggag ctgaaggtcg agcgggacct
     5161 cagccgctct cccggtttcc aggtggtctt cgccttccag aacgtgccgc tcccacgggt
     5221 ctcggcgctg cccgggcttc tcatggagcc cctcgctggc ggcgctccga ccgccaagta
     5281 cgacctggag ctcacgctcg cccaggtgga ggggaggctc gcgggggacc tcctgtacag
     5341 caccgacctg ttcgacgccg tgaccgcgcg tcgtctgctc tcccaatacc gggtcgtgct
     5401 ggcggcgctt gcccagcgcg cgcagagccc gatcggcgag ctgccgtggc tcacgccggc
     5461 ggaggcgcat cagctccgca cggagtggac cgaggtgctc cccgcgccgc taccggcctc
     5521 ctcgatccca gccgctttcg aggagtgggc cgaacggacg ccgcaggccc tggcgctggt
     5581 ctgctccaca gaggagagga cctacggcga tctgaaccgg gaggcgaacc gccttgccct
     5641 tcacctgctg cggctcggcg ccg
//
LOCUS       DASPJI010000676         3702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_556894, whole genome shotgun sequence.
ACCESSION   DASPJI010000676 DASPJI010000000
VERSION     DASPJI010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556894"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="VEL74_19120"
     CDS             complement(<1..1409)
                     /locus_tag="VEL74_19120"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HYG64700.1"
                     /translation="MRQILRSFILVLLVAVAPAPAQENVQETVPAEWRTPAEVAGFEA
                     TPSYADTLAFLERIQKRLPEMRLDFYGRSGQGRRMPVVVVSKEKAFTPEAAARTGKPV
                     LMFLNGIHSGEIDGKDACLMILRDLALGRRRELLDAATLVIVPIYNIDGHERVSPYNR
                     PNQNGPRAGMGFRTNAIGLDLNRDFLKVASPEAQALIGLISAWRPHLHVDNHVTDGVD
                     HDWVLTWSYPEAPQIAAPVDAWVRAHLPAALAATERAGHRHGPYVDLVDRNDPSKGFS
                     SWVGQPRYSSGYFPLRNRPSVLVEMHSYKPYRQRVIANRDFLLALLAEVGRNPGSLVR
                     AVTEAEAATVAKGKPDAAPSEVVVSFKQSETADRIPFPVYAGETKTSVVTGAPLYLFR
                     RGEVAEVQAPWFHRPEVAQSLPRPRGYLVLPGWPQIEQRLRVHGLRVEEVTRPAELDV
                     ETIRVSAPDFGDASYQGLTA"
     gene            complement(1455..1958)
                     /locus_tag="VEL74_19125"
     CDS             complement(1455..1958)
                     /locus_tag="VEL74_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64701.1"
                     /translation="MSSSLSVNDVLGRLQKQLAFHQEQAELHGTQEAHHRGQREHHEE
                     QAEKIARHIEAFREAARPAVDLASQAVPASPAAPKEGVLDTGRRTPVQQLVRDVIQGF
                     APDERFGRSKVMQEIERRHGRNLRRPLEPNRVTIALRRLARLGTIHQVRAGKPHWEAL
                     YVREKAR"
     gene            2547..3689
                     /locus_tag="VEL74_19130"
     CDS             2547..3689
                     /locus_tag="VEL74_19130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64702.1"
                     /translation="MMPTGRSSSRGRPAALLVLLLMTLAAACGGAAETAKSPAAVSDP
                     LAAEIEQAAAFLQSDAAAQGPWAQSREGSLRELGRAQDALRAGRRTLAMFRFSTVKAN
                     LATAAYLSEHATGKDMAAFEAEWKRMGGALKGDLGALSPAALDGVHPAAVRAVGETAL
                     PQIRGYYEASLDYGRSTAPAEGLYYLASAQAQRDLARFARTLSAPASRREPPLRSIAQ
                     ELDALESEMLAAYRPPASIEKHPQFIAASSALKEARELDAAGLCYGALLRYLQAAVRF
                     APLRQDQPALSGDALARRLGEFEKRLAVGDVDHSLGQLFAEIARSDIDGTPADTAGTP
                     SAVAPIAAGIAADVLPRYFAAIEPARPAPARPAPKVTVTLVRWPFT"
BASE COUNT          520 a         1280 c         1317 g          585 t
ORIGIN      
        1 gcggtgagcc cctgatagga ggcatcgccg aaatcggggg cggagacccg gatcgtctcg
       61 acgtccagct cggcgggccg cgtcacctcc tcgacccgca ggccgtgcac gcgcagccgc
      121 tgctcgatct gcggccagcc ggggagcacc aggtagccgc gggggcgggg gaggctctgc
      181 gccacctccg gccggtggaa ccagggcgcc tggacctcgg ccacctcgcc gcgccggaag
      241 aggtagagcg gcgcgccggt gaccacggac gtcttcgtct caccggcata gaccgggaac
      301 gggatgcggt ccgcggtctc gctctgcttg aagctcacca ccacctccga cggcgccgcg
      361 tccggcttcc ccttggccac ggtcgcggcc tcggcttcgg tcaccgcccg taccagggag
      421 ccggggttcc tgcccacctc ggcgagcagg gccagcagga agtcccggtt ggcgatcacc
      481 cgctgccggt agggcttgta ggagtgcatc tccaccaata ccgacggccg gttgcgcagc
      541 gggaagtagc ccgaggagta gcgcggctgt cccacccagg agctgaaccc cttggaggga
      601 tcgttgcggt ccaccaggtc cacgtagggc ccgtggcggt gcccggctcg ctcggtggcc
      661 gccagcgccg cgggcaggtg ggcgcgcacc caggcgtcca cgggggcggc gatctgcggc
      721 gcctcgggat aggaccaggt cagcacccag tcatggtcca ccccgtcggt gacgtggttg
      781 tccacgtgga ggtgcggccg ccatgccgag atcaggccga tgagcgcctg ggcctccggc
      841 gaggccacct tgaggaagtc gcggttcagg tcgaggccga tggcgttggt gcggaacccc
      901 atgccggccc gcggcccgtt ctggttcggc cggttgtagg gggagacccg ctcgtggccg
      961 tcgatgttgt agattggcac gatgaccagc gtcgccgcgt ccagcaactc acggcgccgg
     1021 cccagcgcga ggtcgcggag gatcatcagg caggcgtcct tgccgtcgat ctccccggag
     1081 tggatgccgt tgaggaacat caacaccggc ttgccggtcc gggccgcggc ctcgggggtg
     1141 aaggccttct ccttggagac caccaccacg ggcatgcggc gcccctggcc ggagcggccg
     1201 tagaagtcga gccgcatctc gggcagccgc ttctggatcc gttcgaggaa ggcgagggtg
     1261 tcggcgtagc tcggcgtcgc ctcgaatccg gccacctcgg cgggcgtccg ccactcggcg
     1321 ggtacggttt cctggacgtt ctcctgggct ggggccggag cgacggcaac gagaaggacg
     1381 aggatgaagg aacgaaggat ctggcgcatg gatggagatc tcccgggttt ggaatctacc
     1441 atcagcggca gcgtctaccg cgccttttcc cgcacgtaca gcgcctccca gtgcggcttg
     1501 ccggcgcgca cctggtggat cgttccgagt ctcgcgaggc ggcggagggc gatggtgacg
     1561 cggttgggct ccagcggccg ccgcaggttt cgtccgtggc gccgctcgat ctcctgcatg
     1621 accttggagc gcccgaagcg ctcgtccgga gcgaaaccct ggatcacgtc gcggacgagc
     1681 tgctgcacag gcgtccggcg gccggtgtcc aggaccccct ctttcggcgc ggccggcgag
     1741 gccggcaccg cctggctggc gaggtccact gcgggccgcg cggcctcgcg gaaggcctcg
     1801 atgtgccggg cgatcttctc ggcctgctcc tcgtggtgct cccgctggcc gcggtggtgt
     1861 gcctcctggg tcccgtggag ctccgcctgc tcctgatgga acgccaactg cttctgcagg
     1921 cgtcccagca catcgttgac cgacaagctc gagctcattc ctcgtcatcc tccggcgatt
     1981 ggtgtcgaac cgtctccaat tggtgtcaga tctacgtgga caagtgtcgc acactcgtcg
     2041 ggcgacggct acggtcgttc tctcgacacc aaatcctcgt actggagacg ttccagcggg
     2101 aggcgcgggg ctcacaagag ctggtgaagg cttggaggac caccagttgg acacgaaaac
     2161 tcgtcaacag gtgtcagcca ttcaggtagg aggtgtccgc ctctggggat aggtgtcgac
     2221 ttggcagcgg ttggacacct agggccggca actcgacact accgagtctg atttggtgtc
     2281 gaagtagtgg gtggacccta gtcctgcccc tggagacgtg gcaccgctgt gaggtttggt
     2341 gtcggcatct tgagtactgg tgtcggatcg ggctggtttg gtgacggcta gaggccgagt
     2401 ggacaccaat gcaggtctgt tggacacttg tttatacgga ttggtggcgg ctgaatctca
     2461 attggtggcg gcaggtccag attcagggga gctccgggct ggggcaggct tggggccgga
     2521 ggtccggtcg tggcatcatc cgcggcatga tgcccaccgg tcgaagctcc tcccggggga
     2581 gacccgcggc tctcctcgtc cttctcctga tgacgctcgc cgccgcgtgc ggcggcgcgg
     2641 ccgagacggc gaagagcccg gccgccgttt ccgatccgct ggccgccgag atcgagcagg
     2701 ccgcggcctt cctgcagagc gacgccgcgg cccaggggcc atgggcccag agcagggagg
     2761 gcagcctgcg ggagctgggg cgcgcccagg acgccctgcg cgccggccgg cggacgctcg
     2821 ccatgttccg tttctcgacg gtcaaggcca atctcgccac cgcggcctat ctgagcgagc
     2881 acgccaccgg caaagacatg gcggcgttcg aggccgagtg gaagcgcatg gggggcgctc
     2941 tgaagggtga cctcggcgcc ctctcgcccg ccgcgctcga cggcgtccac cccgcggccg
     3001 tgcgcgccgt gggcgagacg gcgctccccc agatccgcgg ctactacgag gccagcctcg
     3061 actacggccg cagcaccgcg cccgccgagg ggctctacta cctggcgtcg gcgcaggccc
     3121 agcgcgacct cgcgcggttc gcccgcacgc tctccgcccc ggcctcgcgg cgcgagccgc
     3181 cgctccggtc gatcgcccag gagctggacg ctctcgagtc cgagatgctc gcggcgtacc
     3241 ggccgccggc atccatcgag aagcacccgc agttcatcgc cgccagctcc gccctcaagg
     3301 aggcgcgcga gctggacgcc gccggcctgt gctacggcgc cctgctgcgc tacctccagg
     3361 cggcggtccg tttcgcgccg ctgcggcagg atcagcctgc cctctcagga gacgcgctcg
     3421 cccggcgcct gggcgaattc gagaagcgcc tcgcggtggg cgacgtcgac cacagcctcg
     3481 gccagctctt cgccgagatc gcccggtccg acatcgacgg cacccccgcg gacaccgcgg
     3541 gcacgccgtc ggcggtcgcc ccgattgctg ccggtatcgc agccgacgtc ctgccgcgct
     3601 acttcgcggc gatcgagccg gcccggccgg cgcccgccag gccggcgccc aaggtcacgg
     3661 tgaccctggt gcgctggccg ttcacgtgaa acctctccga tc
//
LOCUS       DASPJI010000677         3091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_390415, whole genome shotgun sequence.
ACCESSION   DASPJI010000677 DASPJI010000000
VERSION     DASPJI010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390415"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VEL74_19135"
     CDS             <1..1122
                     /locus_tag="VEL74_19135"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64703.1"
                     /translation="LIARAGEGSVRDSVALLDQLATFGSGGVADEEAVRLLGGLDTAL
                     FHQLLAAILGGEPAGVSAAVRRVEDEGWDPRHVYAQFLAFCRDALHLGMGGDPGQVDL
                     PGEEAAALAALARGSGYENLLRLMHQLLTSEPLVRRSDTGTLAMEIAWLRAAELPKLI
                     RVEEILSAGLPAAPAGAVARAAAPAAAPPAAARSQAAPRPAPATAAPAPAAPSPAPAA
                     APSAPRFSPPPAMAAPPPAPPSARANGAGNSVASFIDEVSRRKQHLAGYLEGADLSFA
                     DGCLTIAVDPDDRILKARLENDANRRILDEAVAAVWGAGNRWRSTVGQSRPNVLAPSP
                     EEAAAQQGVADNAFVQTVLDIFKGRVVEANVEEQSLRED"
     gene            1128..1424
                     /locus_tag="VEL74_19140"
     CDS             1128..1424
                     /locus_tag="VEL74_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HYG64704.1"
                     /translation="MSSMRQLMKQAQEMQERLQRELAEIVVEASVGGGMVTVKMSGHK
                     QLVSVKIDPEAMDPEDPSMLEDLIVAAVNEAGRKVEETMQGRMGSMAASLPGLF"
     gene            1453..2037
                     /gene="recR"
                     /locus_tag="VEL74_19145"
     CDS             1453..2037
                     /gene="recR"
                     /locus_tag="VEL74_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HYG64705.1"
                     /translation="MSDPLARLVGELSRLPGIGPKTATRLAHHLLKVTPDDARALAQA
                     IVEVKEKLFHCSRCNAITAVDPCRFCSDPQRDAARICVVEEPFNIEPMERTGEYHGLY
                     HVLLGALSPHRGIGPDRLAIAGLLERLDGIEEVILATNPNVEGEATALYLARLLKPRG
                     VRVTRLAFGMPVGGDIEYTDEVTLGRSLAGRREM"
     gene            2034..>3091
                     /locus_tag="VEL74_19150"
     CDS             2034..>3091
                     /locus_tag="VEL74_19150"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HYG64706.1"
                     /translation="MRLGDLLPRIVEPPPGLRSRTLARRLSYLEAPAVNTVGPGEPPI
                     LWEEAAGANVLDADGNIYIDLTSGFGVATVGHRHPRVEEAVRRQTGRLLHALGDVHAH
                     PLRVELAARLVRLAPVDDAQVFFAVSGAEAVEIALKTAIGASRKRGIVAFEPSYHGMT
                     LGALAVTSRPEFRALAAEHLHSHVHRLPFGGDPAALEELLAGGDLACVLLEPIVGREG
                     VLVPPPGWLPEVASLCRRAGVLLVADEIFTGFGRTGTLFAVEGEGVRPDILCCGKALG
                     GGLPIAAVVARQALFRCWETEGEALHTSTFIANPLACAAALAVLDILEEENLPARAVR
                     RGIELGRRLAAWPERFPHV"
BASE COUNT          400 a         1170 c         1116 g          405 t
ORIGIN      
        1 ctgatcgccc gcgccggcga ggggagcgtc cgcgactcgg tggcgctgct cgaccagctc
       61 gccaccttcg gctccggcgg cgtcgccgac gaggaggcgg tgcgcctgct cggcgggctc
      121 gacaccgccc tcttccacca gctcctcgcg gcgatcctcg gcggcgagcc ggccggcgtc
      181 tccgcggcgg tgcggcgggt ggaggacgag gggtgggacc cgcgccacgt ctacgcgcag
      241 ttcctcgcct tctgccgcga cgccctgcac ctcgggatgg gcggcgaccc cgggcaggtg
      301 gacctgccgg gggaggaggc cgcggccctg gcggcgctcg cccgcggctc cggctacgag
      361 aacctgctcc gcctcatgca ccagctcctg accagcgagc cgctggtgcg ccgcagcgac
      421 accggcaccc tggcgatgga gatcgcctgg ctgcgcgccg ccgagctgcc gaagctgatc
      481 cgcgtcgagg agatcctgtc cgccggactg cccgcggcgc ccgccggcgc ggtggcgaga
      541 gccgccgcac ctgccgccgc accgccggcg gcggcgagat cgcaggcggc gccccgtccg
      601 gcccccgcca cggcggcccc ggccccagcc gccccgtctc cggcacccgc ggcggcgccc
      661 agcgctcccc gcttctcgcc gcctccggcc atggccgctc cgccgccggc gccgcccagc
      721 gcgcgcgcca acggcgcggg caacagcgtc gcctcgttca tcgacgaggt cagccggcgc
      781 aagcagcatc tcgccggata cctggagggc gcggatctgt ccttcgcgga cggctgcctt
      841 accatcgccg tggatccgga cgaccggatc ctcaaggcgc ggctcgagaa cgacgccaac
      901 cgccggatcc tcgacgaggc cgtggcggcg gtctggggcg cgggcaaccg ctggcgctcc
      961 accgtcgggc agagccggcc gaatgtcctc gcgccgtcgc cggaggaggc cgccgcccag
     1021 cagggcgtgg cggacaacgc gttcgtccag accgtcctcg acatcttcaa gggacgtgtg
     1081 gtggaagcca acgtcgaaga gcaaagcctc agggaggatt gagcccaatg agcagcatgc
     1141 gtcagttgat gaagcaggcc caggagatgc aggagcgtct gcagcgcgag ctcgccgaga
     1201 tcgtggtcga ggcgagcgtc ggcggcggca tggtgaccgt caagatgagc ggccacaagc
     1261 agctcgtctc cgtgaagatc gaccccgaag ccatggaccc cgaggacccc tccatgctgg
     1321 aggacctcat cgtcgccgcc gtcaacgagg ccgggcgcaa ggtcgaggag accatgcagg
     1381 gacgcatggg ctccatggcc gcgagcctgc cgggcctctt ctgagcccac gctgaggcca
     1441 tcgagcccta cgatgtccga tcccctggcc cggctggtcg gtgagctgtc ccgcctgccg
     1501 ggcatcggtc ccaagaccgc cacccgcctc gcccaccacc tgctcaaggt gacccccgac
     1561 gacgcccggg ccctcgccca ggcgatcgtc gaggtcaagg agaagctgtt ccactgctcg
     1621 cgctgcaacg ccatcacggc ggtggacccg tgccggttct gctcggaccc gcagcgcgac
     1681 gcggcccgca tctgcgtcgt cgaggagccg ttcaacatcg agccgatgga gcgcaccggc
     1741 gagtaccacg gcctctacca cgtgctgctc ggggcgctgt cgccccaccg cggcatcggc
     1801 cccgaccgcc tggccatcgc cgggctgctg gagcgcctcg acggcatcga agaggtgatc
     1861 ctcgccacca acccgaacgt cgagggggag gcgacggccc tctacctggc gcggctgctc
     1921 aagccgcgcg gcgtgcgggt gacccgcctc gccttcggca tgccggtggg cggcgacatc
     1981 gagtacaccg acgaagtgac gctgggccgg tctcttgcag gccgccgcga gatgtgagac
     2041 tcggagacct cctcccccgc atcgtcgagc cgccgccggg cctgcgctcc cgcaccctgg
     2101 cgcggcggct ctcctatctg gaagcgccgg cggtcaacac cgtggggccg ggcgagccgc
     2161 ccatcctctg ggaggaggcg gccggcgcca acgtcctcga cgccgacggc aacatttaca
     2221 tcgacctcac ctcgggcttc ggcgtcgcca cggtgggcca ccgccacccg cgggtcgagg
     2281 aggccgtccg ccgtcagacc ggccggctgc ttcacgccct cggggacgtc cacgcccacc
     2341 ccctgcgggt ggagctggcg gcgcggctgg tgcgcctcgc gccggttgac gacgcgcagg
     2401 tcttcttcgc cgtctcgggg gccgaggcgg tggagatcgc gttgaagacc gccatcggcg
     2461 ccagccgcaa gcgggggatc gtcgccttcg agccctccta tcacggcatg acgctggggg
     2521 cgctggcggt gacctcgcgg ccggagttcc gggccctggc cgccgagcac ctgcactcgc
     2581 acgtccatcg cctccccttc ggcggcgacc cggcggcgct ggaggagctc ctcgccggcg
     2641 gggacctcgc ctgcgtgctg ctggagccga tcgtgggcag ggaaggcgtc ctggtgccgc
     2701 cgcccggctg gctgccggag gtggcgagcc tctgccggcg ggccggcgtg ctcctggtgg
     2761 ccgacgagat cttcaccggc ttcggccgca ccggcaccct gttcgcggtc gagggggagg
     2821 gggtgcggcc tgacatcctc tgctgcggca aggcgctggg gggagggctg ccgatcgccg
     2881 cggtggtggc gcgccaggcg ctcttccggt gctgggagac ggagggggag gcgctccaca
     2941 cctccacctt catcgccaac ccgctcgcct gcgcggcggc cctggcggtg ctcgacatcc
     3001 tggaggagga gaacctcccg gcgcgggcgg tgcgccgggg gatcgagctc ggccggcgcc
     3061 tcgccgcctg gccggagcgc ttcccgcacg t
//
LOCUS       DASPJI010000678         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_390434, whole genome shotgun sequence.
ACCESSION   DASPJI010000678 DASPJI010000000
VERSION     DASPJI010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390434"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VEL74_19155"
     CDS             complement(<1..536)
                     /locus_tag="VEL74_19155"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HYG64707.1"
                     /translation="MRLWIARAGSAALSLFTFISTPALGAAPLLAAEPVEVAAGAGEV
                     EVLEDGRGQAIDLQVRFPPRRFTFLPRFVPEVAPILGTIATTRGAIYAYAGLRLDLPL
                     GRRFHFSPSFSGGLYHQGEGQDLGGPVEFRSVVELSYALNERTRVGVSLDHLSNGGLF
                     SSNPGSESLIFLVSSRLG"
     gene            complement(624..>2197)
                     /locus_tag="VEL74_19160"
     CDS             complement(624..>2197)
                     /locus_tag="VEL74_19160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYG64708.1"
                     /translation="AAAVPWVLGAWGLLVGVAMALRPILPVDETRYLSVAWEMWQRGD
                     LLVPHLNGLPYSDKPPLLFWLFHLGWWLFGVNEWWPRLVPALFSLASLFLARSLARRL
                     WPDQAAVARRTPAVLLGLTLWSVYTTLIMFDMLVACAVLVALHGLLTARERGIRGWLV
                     VGAALGIGGLAKGPVVLVMPLTVALLAPWWAGTKVGVRWWAGLLGAVAMGASIALAWA
                     VPAAAAGGGAYARAILFTQTEERLVYSLAHGRPWWWYLALLPVMLFPYSFWVPAWKGV
                     LRLTADAGTRFCLAWLMPGLVVFSLISGKQPHYLLPLLPAVALLIARGMETGGEAGLA
                     ARRWHLAPPLAFLVLVGAAIATARWAERPFRLPTWAGLISPGAGLALLALAAVFVLSY
                     ERLFATRRTAVTLVGLALVVALHVGFAEIARKAYDLEPIARYASVMEWQGRPIAFIAP
                     YHGQLHFLGRLARPFEVIQPGAELVWVHQHPRGKVIQDLDYAPPGLVRADFTQSYRED
                     VLAVWGRESLPQTTP"
BASE COUNT          390 a          757 c          823 g          227 t
ORIGIN      
        1 tggccgagcc ggctgctgac caggaagatc agggactcgc tgccggggtt ggacgagaag
       61 aggccgccgt tggacaggtg gtcgaggctg acgccgacgc gggtgcgctc gttcagggca
      121 taggacagct cgacgaccga ccggaactcc acgggtcccc cgaggtcctg cccctccccc
      181 tggtgataga gaccgccgga gaagctgggg gagaaatgga agcgccttcc cagcggcagg
      241 tcgaggcgga gcccggcgta ggcgtagatc gccccgcggg tggtggcgat ggtgcccagg
      301 atgggagcga cctcgggcac gaagcggggc aggaaggtga aacggcgggg tgggaaccgc
      361 acctgcaggt cgatggcctg gccccgcccg tcctcgagca cctcgacctc gccggcgccg
      421 gcggccactt ccaccggctc ggcggccagg agaggagcgg cgccgagcgc cggcgtcgat
      481 atgaatgtga atagagacag agcggcggag cccgctcgcg cgatccagag cctcatcttc
      541 ccctctcgca cccggcccgg gtgcagccgg agcactatac ccctggatat agcattggag
      601 aaaccggtgc cgcaagcggg tctctagggc gtggtctggg gcagcgactc ccggccccag
      661 accgccagga cgtcttcccg gtaggactgg gtgaagtcgg cgcggacgag gccgggcgga
      721 gcgtagtcca gatcctggat caccttgccc cgcgggtgct ggtggaccca gaccagctcc
      781 gccccgggct ggatgacctc gaatggacgc gcgagccggc ccaggaagtg gagttggcca
      841 tgataagggg cgatgaacgc gatcggcctc ccctgccact ccatcacgct ggcatagcgg
      901 gcgatgggct ccaggtcgta ggctttccgg gcaatctcgg cgaagccgac gtggagggcg
      961 accaccagcg ccaggccgac gagggtgacg gcggtgcgcc gcgtggcaaa gagccgctcg
     1021 taggagagga cgaagaccgc cgccagggcc aggagggcca ggccggcacc gggcgagatc
     1081 aggcccgccc aggtcggcag ccggaagggc cgctccgccc accgggccgt ggcgatggcc
     1141 gcgcctacca ggaccaggaa ggcgagaggg ggtgccaggt gccagcgccg ggcggcgagc
     1201 ccggcctcgc cgccggtctc catgccgcgg gcgatcagca gggcgacggc cggcagcagc
     1261 ggcaggaggt agtggggctg tttgccgctg atcagggaga agaccaccag gcccggcatc
     1321 agccaggcga ggcagaagcg ggtgccggca tccgccgtca gccggaggac ccccttccag
     1381 gccggcaccc agaaggagta ggggaagagc atcaccggca acagcgccag gtaccaccac
     1441 caggggcggc cgtgggcgag ggaatagacg agccgctcct cggtctgggt gaacaggatg
     1501 gcccgggcat aggcgccgcc gccggccgcg gcggccggca cggcccaggc cagggcgatg
     1561 gaggccccca tcgccaccgc gcccagcagc cccgcccacc agcgaactcc cacttttgta
     1621 cctgcccacc agggggcgag cagcgccacg gtcagcggca tcaccaggac caccggcccc
     1681 ttggcgagcc ccccgatccc gagcgccgcc cccaccacca gccagccgcg gatcccccgc
     1741 tcgcgggcgg tcagcaggcc atggagcgcc accaggacgg cgcaggccac cagcatgtcg
     1801 aacatgatca gggtggtgta caccgaccac agggtcaggc cgaggagcac cgcgggggtc
     1861 cgccgggcga ccgccgcctg gtccggccag aggcggcggg ccagggaacg ggcgaggaac
     1921 aggctcgcca ggctgaacag cgccggcacc aggcgcggcc accactcgtt gacgccgaac
     1981 agccaccagc ccaggtggaa cagccagaag agcagcggcg gcttgtcgct gtagggcagc
     2041 ccgttcaggt gcggcaccag gaggtcgccg cgctgccaca tctcccaggc cacggagagg
     2101 tagcgggtct cgtcgaccgg gaggatgggg cgcaacgcca tggccacgcc gaccaggagg
     2161 ccccaggcgc cgagcaccca ggggaccgcc gccgcct
//
LOCUS       DASPJI010000679         4282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_442595, whole genome shotgun sequence.
ACCESSION   DASPJI010000679 DASPJI010000000
VERSION     DASPJI010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442595"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VEL74_19165"
     CDS             complement(<1..987)
                     /locus_tag="VEL74_19165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG64709.1"
                     /translation="MTLSIRDAQDPAALEAFLGLPDEVYAGDPHYVPISRETVTAALR
                     RPELEEAQRVLVAMEGGRPVARLAAWRSPALRDEAGRPYGMLGLFEALDRPEAAGRLF
                     EEAIGWLRRTGAGPIVGPMDGDTWHRYRLNMGPWDDPPFLLEPYNPRYYPALWEGHGF
                     EVLERYLSTRVDLGSALAHLVPRHREAIAAGYVLRRLDLSRFEDELRLLYRLSRVIFA
                     DNFLYTEIAEEDFLALYTGARRLLDPDLVQFAAAPDGQPVGFLFAYPDRFQAVAAMRG
                     ERGPLALVRYLRHRRTDTVDFKTLGVLPEHRRSGVAAALMGQGHLEALRKGYR"
     gene            complement(995..2581)
                     /locus_tag="VEL74_19170"
     CDS             complement(995..2581)
                     /locus_tag="VEL74_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64710.1"
                     /translation="MATVQELSVLHRRYSDISHRFRAAWTFHQFFQSLAKSLLQRPED
                     GRAGDFQRLYTELKEISQSLNASEGARVRGRLDSIDRQITKLIEDLAEEDTRVSPELM
                     RQFFRRVKGYDEKILTQLVKFYLYAQHGDFWEDDRLDKLDFLLTRMSEEEMETGELML
                     TDPRRLGEIFAGLWAMLGLDPSPATIQGRRAEVEAIRGELSRVESLDDLNEAGLIQRY
                     RDLKHGLGSLFFEPGLLISLQETNLALRNRIQKLYSREERRIAAEYQRIFELEREVPV
                     DTELDRELAQFREEIERFEKQLQRQEFKLGDLAQIRQRVRSLLPRLTAIRPELTGRSV
                     GGTLPGLDSGEHRIAAATAAASSPLPVSAREDVIGEHYRRLVDALRETNPDMAPERLV
                     LVPEIYPLRLEAREVLAYRRFFGRKDHDRVLEQFLLEAAALRIRINEEAHEITGILDE
                     TSITGDSPIYGRARLTCRTADEYLWRFHHVLHDTLLGGDPGEALQIEVLRMRLMRDYS
                     GLWLLAYKPFVTRRPGHTVA"
     gene            2643..3182
                     /gene="hpt"
                     /locus_tag="VEL74_19175"
     CDS             2643..3182
                     /gene="hpt"
                     /locus_tag="VEL74_19175"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HYG64711.1"
                     /translation="MKVLIRQEDLERRIGEMAAEIERDYAGCDNLMCVGVLKGSVFFM
                     VDLLKRISIPLAVDFFQTSSYGSGTTPGEVRIRKDVDLSIRGKDVLLIEDIVDTGYTL
                     RTILDLLRFRGARSVRLCALLDKASRRQVDVPIDYLGFEIEDLFVVGYGLDFDERYRN
                     LPYIAVWEPDEPSDVPSPS"
     gene            3201..3617
                     /locus_tag="VEL74_19180"
     CDS             3201..3617
                     /locus_tag="VEL74_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005871936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HYG64712.1"
                     /translation="MKFHHTDSAPKAIGPYSQSVSLDGWLYTSGQVGIDPATGELVPG
                     GFEAQAKRVFENLKQVLASAGCTFQDVIKATVYVVDMADFPKLNAIYADAMGEHRPAR
                     STVQVAALPKAALVEIDLVAKTQAAPASGGSIRGTL"
     gene            complement(3623..>4282)
                     /locus_tag="VEL74_19185"
     CDS             complement(3623..>4282)
                     /locus_tag="VEL74_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HYG64713.1"
                     /translation="NTHNMAGGTLWERPRTDAVLEVARRHRLPVHLDGARLFNAAVAL
                     GTTPAALAAGFDSVMFALSKGLGAPVGSLLCGSRAFVDEARRVRKMFGGGMRQVGILA
                     AAGLIALRKGPSWLADDHANAALLAGALAELPGIELDPAAVRTNIVIFRVTPAFFGGD
                     TPAEGLSNAFLARLKEEGVLATPVTHELVRLVTHRDVPRPRVEEAIERLRRCAGALAS
                     R"
BASE COUNT          661 a         1449 c         1563 g          609 t
ORIGIN      
        1 ccggtagccc ttgcgcagcg cttcgaggtg gccctggccc atcagcgcgg cggccacgcc
       61 cgagcggcgg tgctccggga gcacgcccag ggtcttgaag tcgacggtgt cggtgcggcg
      121 gtgccggagg tagcggacca gcgccagggg gccgcgctcc ccccgcatgg ccgcgacggc
      181 ctggaagcgg tccggatagg cgaagaggaa gccgaccggc tgcccgtccg gcgccgcggc
      241 gaactggacg aggtcggggt cgagcaggcg ccgggctccg gtgtagaggg cgaggaagtc
      301 ctcctccgcg atctcggtat agaggaagtt gtccgcgaag atgacccgcg acaggcggta
      361 cagaagccgc agctcgtcct cgaagcggga gaggtccagg cggcgcagca cgtaaccggc
      421 ggcgatcgcc tcgcgatggc gcggcacgag gtgggccagc gcgctcccca ggtccacccg
      481 ggtggagaga tagcgctcca gcacctcgaa gccgtgcccc tcccagagcg ccgggtagta
      541 gcgcgggttg tagggctcga ggagaaacgg cggatcgtcc cagggcccca tgttgagacg
      601 ataccggtgc caggtgtcgc cgtccatggg gccgacgatg ggaccggcgc cggtccgccg
      661 cagccagccg atcgcctcct cgaacaggcg cccggcggcc tcgggccggt ccagggcctc
      721 gaagaggccg agcatgccgt aggggcggcc ggcctcgtcc cggagcgccg gcgaccgcca
      781 ggcggccagc cgcgccacgg gtcggccccc ctccatcgcc acgaggaccc gctgggcctc
      841 ctccagctcc ggccgccgga gcgcggcggt caccgtctcg cgcgagatgg ggacgtagtg
      901 ggggtctccc gcgtagacct cgtccggcag gccgaggaag gcctccagcg cggcggggtc
      961 ttgggcgtcg cggatggaga gggtcaaatc cccctcacgc caccgtatgc ccgggccggc
     1021 gggtgacgaa gggcttgtag gccaggagcc agaggcccga gtagtcgcgc atcaggcgca
     1081 tgcggaggac ctcgatctgc agcgcctcgc ccgggtctcc ccccagcagg gtgtcgtgga
     1141 ggacgtgatg gaagcgccag aggtactcgt cggcggtgcg gcaggtgaga cgagcccggc
     1201 cgtagatggg cgagtcgccg gtgatggagg tctcgtccag gatgccggtg atctcgtgcg
     1261 cctcctcgtt gatgcggatg cgcagggccg ccgcttcgag aaggaactgc tccaggacgc
     1321 ggtcgtggtc cttgcggccg aagaagcgcc ggtaggccag gacctcgcgg gcctcgagcc
     1381 gcagcggata gatctcgggg accagcacca gccgctccgg agccatgtcc gggttggttt
     1441 cgcgcagggc gtcgaccagc cggcgatagt gctcgccgat gacgtcctcg cgcgccgaga
     1501 ccggcagggg ggacgaggcg gcggcggtgg cggcggcgat gcggtgctcg ccgctgtcca
     1561 ggcccggcag ggtcccgccg acgctccggc cggtgagctc ggggcggatg gcggtgaggc
     1621 gcggcagcag cgaccgcacc cgctggcgga tctgggccag gtcccccagc ttgaactcct
     1681 ggcgctggag ctgcttctcg aagcgctcga tctcttcgcg gaactgggcc agctcgcggt
     1741 ccagctcggt gtccaccggc acctcgcgct ccagctcgaa gatgcgctgg tactcggcgg
     1801 cgatgcgccg ctcctcgcgg ctgtagagct tctggatgcg gttgcgcagc gccaggttgg
     1861 tctcctggag cgagatcagg aggcccggct cgaagaacag gctgcccagg ccgtgcttga
     1921 ggtcgcggta gcgctggatc aggccggcct cgttgaggtc gtccaggctc tcgacccgcg
     1981 acagctcgcc gcggatcgcc tccacctcgg cgcggcgccc ctggatcgtg gcgggggacg
     2041 ggtccagccc cagcatggcc cagaggccgg cgaagatctc ccccaggcgg cgggggtcgg
     2101 tgagcatcag ctcgccggtc tccatctcct cctcgctcat ccgggtgagc aggaagtcca
     2161 gcttgtcgag gcggtcgtcc tcccagaaat cgccgtgctg ggcgtagagg tagaacttga
     2221 cgagctgggt gaggatcttc tcgtcgtacc ccttgacgcg ccggaagaac tgccgcatca
     2281 gctcggggga gactctggta tcctcctccg ccaaatcctc gatcagcttg gtgatctggc
     2341 ggtcgatgga atccagccgg ccacggacgc gcgccccttc cgaggcgttg aggctctggg
     2401 agatctcctt gagctcggta tagagtctct ggaagtcgcc ggcccgcccg tcctccgggc
     2461 gctgcagcag ggacttcgcc aagctctgga agaactggtg aaacgtccac gccgcccgga
     2521 agcggtggga gatatccgag tagcgacggt ggaggacgct cagttcctgg acggtagcca
     2581 ttgagaacca acaataagtt gagaggatct tacgtcatgg cgagggcaaa ggggaacagg
     2641 ggatgaaggt cctgattcga caagaggacc tggagcggcg gatcggcgag atggcggccg
     2701 agatcgagcg cgactacgcc gggtgcgaca atctcatgtg cgtcggcgtc ctcaagggct
     2761 ccgtcttctt catggtggac ctgctcaaga ggatctccat cccgctggcg gtcgacttct
     2821 tccagacgtc gagctacggc agcggcacga cgcccggcga ggtgcgcatc cgcaaggacg
     2881 tggacctctc catccgcggc aaggacgtcc tgctgatcga ggacatcgtc gacaccggct
     2941 acacgctgcg gacgatcctc gacctgctgc gcttccgggg ggcgaggagc gtccgcctct
     3001 gcgccctgct cgacaaggcc agccggcggc aggtggacgt ccccatcgac tacctcggct
     3061 tcgagatcga ggacctcttc gtggtgggct acgggctgga cttcgacgag cgctaccgca
     3121 acctccccta catcgccgtc tgggagccgg acgagccctc ggacgtgcca tccccttcgt
     3181 aacaggagat tcggaacccg atgaaattcc atcacacgga cagcgccccc aaggcgatcg
     3241 gcccctactc ccagtcggtg tcgctcgacg gctggctcta cacctcgggt caggtgggca
     3301 tcgacccggc gaccggcgag ctggtgcccg gcggcttcga ggcgcaggcg aagcgcgtct
     3361 tcgagaacct caagcaggtg ctggcgagcg ccggctgcac cttccaggac gtgatcaagg
     3421 cgacggtcta cgtcgtcgac atggccgact tccccaagct gaacgccatc tatgccgacg
     3481 cgatgggcga gcaccgcccc gcccgctcca ccgtccaggt ggcggccctc cccaaggccg
     3541 ccctggtgga gatcgacctg gtggcgaaga cgcaggcggc ccccgcctcg ggcggcagca
     3601 tccgcggcac gctctagaga actcagcgcg acgcgagggc tccggcgcaa cgccggagcc
     3661 gctcgatcgc ctcctccacc cgcggccgcg gcacgtcgcg gtgggtgacc aggcggacga
     3721 gctcgtgggt gaccggggtc gccagcaccc cctcctcttt caaccgggcc aggaaggcgt
     3781 tggagagccc ctcggcgggg gtgtcgccgc cgaagaacgc cggggtgacg cggaagatca
     3841 cgatgttggt ccgcaccgcg gcggggtcga gctcgatccc cggcagctcg gccagcgcgc
     3901 cggccaggag cgcggcgttg gcgtggtcgt cggccagcca ggacggcccc ttgcgcaggg
     3961 cgatgagccc ggcggcggcg aggatcccga cctggcgcat gccgccgccg aacatcttgc
     4021 gcacccgccg cgcctcgtcg acgaaggcgc ggctgccaca gagcagcgac ccgaccggcg
     4081 cccccagccc cttggacagg gcaaacatca ccgagtcgaa gccggcggcg agggccgcgg
     4141 gcgtggtgcc gagggccacg gcggcgttga agaggcgcgc gccgtcgagg tgcaccggca
     4201 gccggtgccg ccgcgccacc tcgaggaccg catcggtgcg cgggcgctcc cagagggtgc
     4261 cgcccgccat gttgtgggtg tt
//
LOCUS       DASPJI010000680        17129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_501591, whole genome shotgun sequence.
ACCESSION   DASPJI010000680 DASPJI010000000
VERSION     DASPJI010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17129)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17129
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501591"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            456..635
                     /locus_tag="VEL74_19190"
     CDS             456..635
                     /locus_tag="VEL74_19190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64714.1"
                     /translation="MSQPETSERAIWAKGPDKDHKEKANHGHTRSKEKDDTGDDGPGP
                     SCCAPTDPDPDPDEG"
     gene            903..3575
                     /locus_tag="VEL74_19195"
     CDS             903..3575
                     /locus_tag="VEL74_19195"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYG64715.1"
                     /translation="MIQRHLAKRPGDPWLTLVLAGLDRSRSEALYRVAARSFAARKHA
                     QGEVLARSNLQRIFFEQGRLEEAGEQVEVAERIALASRDPEVIAQAHILMARHLTGID
                     KDLERAYLLLRDAEHQVFSAGTDNTKRAWLRAMGNVSQELGRFQEAEDAFRRLAEHAA
                     STNDKSAEGSARYGLAISFFDSVAELPSEKDRRNALELARQTLQPAREGEAFGALSKA
                     YWMIALLAEGEEARRSVEGCLDAAPTLRDQSYCFNALARQLTARDPKAAREATDRALA
                     LAQQAEDPFSMAAALRERMRVSWEAVSVERAVADSWAALDSIEEVRDLQAGSASQAEM
                     FSSWAEDYYWFCGRLLEAFAEGGGARELEGAFAVAERLRARSLIDALEAAKAAPAASP
                     TAQVLRRQRSQVLEAISAVQRRLMDPALPAAERAAAAEQLEDLEMKEANLRNGIAHAD
                     PVYTALRRPEFATLEQVRQALAPDEALLSFQIAPWQDLRGDFAGGSWLLVSTRASTRA
                     YRLPGRGDLRPAVRLFVGTFEQRDGSEVGPSVYLYNELVGKALAELPGGIRRLVLIPD
                     DALHQLPFGALRASADAPPLAARYQMSVVPSATLWLGWRQRRPGAAPNPALALADPPP
                     LSGEEAGEEAEAAPAAERSAVFASAMRLGSLPYARRESRAVVRAMGGGSVRRLGKDAS
                     ETFFKRADLARFGLIHLATHAVVDEQNPERSGVLLAAAGGDDGLLQLREIVDLPLDGR
                     IVVLASCRSASGTLLRGEGVMGMARAFFQAGSHAVVASLWPLRDDEAALLFDRVYHYL
                     GRGDRLAAALQAAQRDLIADGVPAAAWAGLVVLGDGDLVPLPGGRKLPYLSPAEKALA
                     AAVLLALLGAGALAWYRRRRTRRGDVHTVPSPSV"
     gene            complement(4017..5261)
                     /gene="glf"
                     /locus_tag="VEL74_19200"
     CDS             complement(4017..5261)
                     /gene="glf"
                     /locus_tag="VEL74_19200"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HYG64716.1"
                     /translation="MFDYLIVGAGFAGSVLAERLARGAGKRVLLVDRRPHIGGNAYDR
                     HDSAGVLIHQYGPHIFHTNSREVFEYLSQFTEWRPYEHRVRASVDGQLVPIPINLDTI
                     NQLYGLKLNSMEVEKFFESVAEPRENVRTSEDVVVSRVGRELYEKFFRGYTRKQWGLD
                     PSQLDSMVTARVPVRTNRDDRYFSDTYQAMPLHGYTRMFENMLDHENIKILLNCDYRE
                     IVEEVDYDKLVYTGPVDEFFDFRYGPLPYRSLDFKFETVNQEWVQPVAVVNYPNEHLY
                     TRVTEFKHLTGQEHKKSTLVYEYPMAEGDPYYPVPRPENQELYKKYQALAESTQDVHF
                     VGRLATYKYYNMDQVVAQALSVYARIVGQKRAEASRTTLAVPTAAAVAAVTTTVTATV
                     ATAARKERRRAVGGTRKLAKGA"
     gene            complement(5361..6521)
                     /locus_tag="VEL74_19205"
     CDS             complement(5361..6521)
                     /locus_tag="VEL74_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYG64717.1"
                     /translation="MPFSDQAGDRKPDLVCLSHLRWDFVYQRPQHLMSRFARDRRVFF
                     VEEPMPHDGPPRLEVSDRPEGVRVVVPRLPHGLDAQAAEAAQRELLPSLFAEQGIADY
                     ILWYYTPMALAFTAGLAPSAVVYDCMDELSLFRGAPPELLEREARLLESADLVFTGGQ
                     SLYEAKRDRHPDVHAFPSSIDAEHFGRARQLRGSEPADQAGIPRPRLGYFGVIDERLD
                     LDILAGVADARPDWHLVILGPVVKIDPASLPRRANLHYPGMKKYEELPDYLAGWDVAL
                     MPFARNESTRFISPTKTPEYLAGGRPVVSTPIRDVVRPYGEMGLVEIAEDVPGFVAAA
                     ERALDMGRGAGEAREEWLRQVDGFLAGSSWHSTWQRMSELIESAVAARRRVA"
     gene            complement(6508..7674)
                     /locus_tag="VEL74_19210"
     CDS             complement(6508..7674)
                     /locus_tag="VEL74_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015178623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HYG64718.1"
                     /translation="MLTKSFWIGGFECACHRRRDGRRLDLIEATGHDRWLAADYERLR
                     RLGMTTAREGVRWHLIEPSPGRYDFSSLLPMVRTARDLGIQVIWDLCHYGWPDDLDVF
                     GARFADRFAAYAREVARVVREETGEPPLWAPVNEISFLSWAGGDVEYLNPFCRGRGFE
                     LKVQLVRATLAAIDAVWEVDPRARIVHADPAIHIAADPRRPEEAAAAEEQRLCQFQGW
                     EMLRGGIWPQLGGAAPYLDVLGVNYYPGNQWVLNGGKIQRGEPLYRPFRAIVQEIWER
                     FGRPMLITETGAEGEDRAPWLRYVSEEVRAARRAGVPVHGICLYPILDYPGWDDDRHC
                     PAGLWGFCGDEGEREEHGSLAMELELQTELFRREEGTAEVQLATTEEEIEHAVF"
     gene            7921..9303
                     /locus_tag="VEL74_19215"
     CDS             7921..9303
                     /locus_tag="VEL74_19215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64719.1"
                     /translation="MSAHYHPDRSALERFARGRSSAVEEARIESHLRTGCGLCQAEVD
                     RLLPADPEVSTGRPAGAKERVKMAAGLEESGWERVLGELERRLASVIQEREAAPQRVA
                     EILGRPTEARHELIASDRRFHSLAVCELLIDQSFDEGFQDPARALQTAELAVAVATRL
                     ETGHYGCSVVHDLQARAWAYLGNACRIRGDFAEAERALARAEALAEAGSADPLEEARI
                     LDLRASLLADQGWLEEASDLLDVVIDICDEIRDLHRKGRALLSKGILQGDAGEPERAV
                     ELITAGLSLIRWEEEPRLVLVARHNLIAALVACGRCEQARLQLEHLRLSVRDASSSWM
                     EPHLLWIEGRLAARQDRLDEAEALLLEVQRLLLEQGRDYDATLVILDLAKVYLRQGKH
                     LDIRRLADHMLPLLLNHDGCHQAITALVTFQQAAEMDRLTPHLIQGIASYLLRARKNP
                     KLPFHEAATA"
     gene            9400..10620
                     /locus_tag="VEL74_19220"
     CDS             9400..10620
                     /locus_tag="VEL74_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HYG64720.1"
                     /translation="MATPLPLAPVVPAATARRLLMDAQELLADPRRRATAGTLYEIIE
                     RMGFVQIDSINVVERAHHLTLAARLQGYRPALLDRLLERERRLFEHWTHDASAIPSLW
                     YPYWRHRFERYREKVLHHPWWRERVGPDPESMAEQVRQRIAREGPVMTRDFEDTRGEG
                     AEKTWWGWKPEKAVLEYLWRTGELAIAGRVNFHKVYDLAERVFPHLHTAPRPTDAEHL
                     DWACRTALERLGVATADEIAGFWRAVSLDEARSWGREAAARGEIAAVQVEAVDGSKPR
                     SAWAVPDWAERAAAAPAAPPRLRLLSPFDPILRDRKRTLRLFGFDYRFEAFVPAPQRR
                     YGYYVLPMLEGERLVGRLDPKLRRDEGLLEIRKVWWEPGVRQSRGRLAALEAAVARLA
                     GLVGAERWEIAGSD"
     gene            complement(10630..12771)
                     /gene="ppk1"
                     /locus_tag="VEL74_19225"
     CDS             complement(10630..12771)
                     /gene="ppk1"
                     /locus_tag="VEL74_19225"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HYG64721.1"
                     /translation="MSELKTLPFERPLGPAEPAEDAVPLFLNRELSWLEFNRRVLHEV
                     EDPSIPLLERLKFVAIFDSNLDEFFMKRVGGLKQQLASNVRDLPPDGGSPRQQLAAIN
                     AAVRPMLALERRLLNEELLPALRQHGLEILTWDELKASERRHLVEEFDRKIFAILTPL
                     AVDPAHPFPFISNLSLSLAVSVKAPGEDEVSFARVKAPHLLPRWIQIPKTLRFVPLEE
                     VIAHNLDRLFPGMEIVESYPFRVTRNADVQRNEETADDLLEAIQEELRERRFATVVRL
                     EVAAGMPGWMLDLLSEELEVVPQEIFEAEGPLALRDLMTLASSVPLPSLRYRPWAPVT
                     HPRLHPPAPDEATDIFATIAAGDFLVHHPYDSFATSVQRFIEAAADDPAVFAMKQTLY
                     RTSAESPNMQALIRAAEMRKQIAVTVEIKARFDEAANIEWAEALEKVGAHVAYGLVGL
                     KTHAKIALVVRQERDATLRCYCHIATGNYNPETAKLYTDLGLFTCREDITEDVAQLFN
                     LLTGYVYQPRFKKLLVAPINMRPRFLELIAREVEHQKAGRGGRIIAKMNALDDRQIIE
                     ALYEASAAGVEIDLIVRGICRLRPGLPGRSETLRVISIVGRFLEHTRIFYFANAGAPE
                     HFIGSADWMSRNLDARVEAIVPVEEPRLQEEIKTILDLQLADNVKAWDMRPDGSYVQR
                     RPAPGEDARSSQDLLMQRALDRAAERRRVDT"
     gene            12880..14538
                     /locus_tag="VEL74_19230"
     CDS             12880..14538
                     /locus_tag="VEL74_19230"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HYG64722.1"
                     /translation="MATDATADLTAQPLKFPGADPSGHDAGAAPPAGERLAAIDIGSN
                     SIHMIVVAAEPNGGYRVLGREREMVRLGKSGLSEGALSEAAMRDGLEALVKMTTLARL
                     KGAERVVAVATSAVRESANGQDFLARVKAQTGLDVQLLTGVEEGGLIYRAVREVVDLG
                     PGSAVILDIGGGSTEWITSRAGELDRVVSLTLGSLRCAAELKGDPPTAGSIDRLRRRV
                     RERLAEAVPGIQVERLVATSGTAVCCADLVDLFASRPWKEQSGALREVRVKDLAALVE
                     RLRLLRRAEIANLPPVGGPRSDSLLAGAILLQEQAAHAGVERFLVSDRALREGLVLAA
                     LGRPIPSATEPEDLRRRQILQLAERTESVLRHNLQSARLAVRLFDVTASLHGLGQRER
                     EWLEYAALLHDIGYSIHYRSHHKHTFYLIHNANLDAFDQREIEIIAHVARFHRGASPK
                     PEHPDLADLKPWQQRTIRQLVALIRLADSLDRTHASRVDEIYAAIRGGRSGKSGRSCR
                     VTLEVLSRYDVELELEAARHHGRLFEKVFGCKLRLRQGLEVAGG"
     gene            14885..15571
                     /locus_tag="VEL74_19235"
     CDS             14885..15571
                     /locus_tag="VEL74_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG64723.1"
                     /translation="MAHERILVIEDEPDIAEVLQYNLEKEGFRVETARRGDSGLEAVR
                     SDTPDLIVLDLMLPGLDGLELARVLKRDSTTSRIPIVMLTARGEEVDRIVGLELGADD
                     YISKPFSPREVVLRVKAVLRRRQPEEAPASERLELGGIELDVAAHQLRVRAKEIPLTA
                     TEFRLLRLLMERCGRVQTRGQLLSDVWGYAEDIDSRTVDTHIRRLRRKLGPEADRIET
                     VIGVGYRLRP"
     gene            15577..>17129
                     /locus_tag="VEL74_19240"
     CDS             15577..>17129
                     /locus_tag="VEL74_19240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HYG64724.1"
                     /translation="MRLLGFPRKLRLAPFWPALAAVLTASLLLWQLLPGLFERGASAQ
                     LLDTLQILTRLVSQRLTASGEDVDGPVAADLEAWVRDITAESDLRITLIRADGHVIAD
                     SSRTVEQVPGMENHAGRPELQEALRRGQGVAVRRSETTGLTYVYAARTLTVPRAGGAP
                     GESGLLVLRLAQPLAQVEALRGGLAAAMGLAAAAAGLVIFVVSWWIGRRLFDPLARLI
                     VGAGDLASGRKEQVEVPDEEELAPLARALNHLAETAAAQFAAVRQERDHLMAILASMS
                     EGVLVVDGEGRALLVNPAFRRLFDLAGEVTGRPVLEIVRLPELARVVADTLRLGEGQG
                     GQVELAAPEKRTLLLASAALSGGGRGAVVVARDTTELTRLGDMRRDFVANVSHELKTP
                     LAAIRGYAETLRDGALDEPPIARRFTERILWQCRRLQALLDDLLTLSRLESVTIPRER
                     EPVDLHAVVRRMAELLSAAAREKQVRIEVEEKPVPPVLGDADGIERMLSNLLDNAIKY
                     NRPDGRVTVR"
BASE COUNT         2512 a         6149 c         5941 g         2527 t
ORIGIN      
        1 tgaaggtggt caccgaggtc caggggctgc ggaccaccgc ctacaaggtg ccggagaact
       61 cgctcgccgg cctccccccg ggggcgaaga tcctctggcg cgtcgaggcg gtcctgagcg
      121 acggcagccg cgacggctcc cccaccttca ccaccaccct gcagtaaccc gccccccgcg
      181 ccgctcacgg ccgcgggggg atctccccat cggtctcccc agctcctgca acgccgcttg
      241 acgaagcgga tgaagaggtc gtaaacctct gactccgtgg aatttccggt cttggccgcc
      301 ggttttcgac ggcgatccag aggacgtaga acaaaattcg ccggaccggc gtattgggtg
      361 aggggattgc ccctcctcct tcgttctact ggacaggggc ggcgaggccg acccgaggat
      421 ggatgaacct gaccgttgaa atgaggagcc aaaccatgag ccagccagag accagcgagc
      481 gcgcgatctg ggccaagggc cccgacaagg accataagga gaaggccaac catggccaca
      541 cccgttcgaa ggagaaggac gacaccggcg acgatggccc cggcccctcc tgctgcgctc
      601 cgacggatcc ggaccccgat ccggatgagg gttagatcct cttggcgggc ggccgctagg
      661 ccgcccgccc ccagccgctc gggcctctcc tccagcaagt gcagtacact tcgggcttga
      721 tgcgccgcct cgcgctcctc tctctctctt tcctcctggc cctctcactc gccgccttgg
      781 gccagagcac ggactttgca gcctgtgaag cccttttcac ccccgccgga gacagcgagg
      841 aggctgcgag gtgtatctcc atgactggcc ggagactgga tcggcgcgca gaagcggccc
      901 agttgataca acggcacctg gcgaagcggc cgggcgaccc ttggctgacc ctggtcttgg
      961 ccggcctcga tcggagccgg tccgaggcgc tgtaccgggt ggccgctcgg agcttcgcag
     1021 cccgtaagca cgcccaagga gaggttctgg cccgttccaa cctacagcgc atcttcttcg
     1081 agcaggggcg cctcgaggag gcgggtgagc aggtcgaggt cgcggaaagg atcgcgctgg
     1141 cttctcgcga ccccgaggtc atcgcccagg cgcacatctt gatggcgcgg cacctcaccg
     1201 ggatcgacaa ggatcttgaa cgcgcctatc tcttgctccg tgacgccgag caccaggtgt
     1261 tctccgccgg aaccgacaac acaaagcgcg cctggctaag ggcgatgggg aacgtcagcc
     1321 aggagctggg acgcttccag gaggccgagg acgccttccg gagacttgcc gagcatgccg
     1381 ccagcacgaa cgacaagtcg gccgagggca gcgcccgcta cggcctcgcc atatcgttct
     1441 tcgacagcgt cgccgagctg cccagcgaaa aggaccgcag gaacgccctg gagctcgccc
     1501 ggcagactct gcagccggcc cgcgagggag aagccttcgg cgctctatcg aaggcttact
     1561 ggatgattgc cctgctggcc gaaggcgagg aggcccggcg aagcgtcgag ggctgcctcg
     1621 acgcggcccc tactctgcga gatcagagct actgcttcaa tgccctagcc cgccagctca
     1681 ccgcgcgcga tccgaaggct gcccgggagg cgacggaccg cgcgctcgct ttggcgcagc
     1741 aggccgaaga ccccttctcc atggcggccg cgttgcgtga gcgcatgcgg gtaagctggg
     1801 aggccgtctc cgtagagcgg gcggtcgccg attcctgggc cgcgctcgac agcatcgagg
     1861 aggtgcgcga cctccaggca ggctccgcca gccaggccga gatgttctcg agctgggccg
     1921 aggattacta ctggttctgc ggccggctgc tggaggcatt cgcggagggc ggtggggcac
     1981 gggagctgga gggcgccttc gccgtggccg agcgcctgag ggcccgcagc ctgatcgacg
     2041 ctctggaggc cgccaaggcg gctcccgcgg cttccccgac cgcccaggtg ctccgccggc
     2101 agcgttccca ggtgctcgag gccatctccg ccgtccagcg gcggctcatg gatcccgccc
     2161 tcccggcggc tgagcgggcc gcggcggccg agcagctcga ggacctggag atgaaggagg
     2221 cgaacctccg caacggcatc gcccacgccg atccggtcta caccgccctg cgcaggcccg
     2281 agttcgccac cctggagcag gtgcggcagg cgctcgcgcc ggacgaggcc ctgctctcct
     2341 tccagatcgc cccctggcag gacctgcggg gggacttcgc cggcggctcc tggctcctgg
     2401 tctcgacgcg cgcctccacc cgcgcctatc gcctcccggg gcgcggcgac ctgcgcccgg
     2461 cggtgcgcct gttcgtcggc acgttcgagc agcgcgacgg ctcggaggtc ggaccctccg
     2521 tctatctcta caacgagcta gttggaaagg ccttggccga gctgcccggc ggcatccgcc
     2581 ggctggtgct catcccggac gacgccctgc accagctccc cttcggcgcg ctccgggcct
     2641 cggcggacgc gccgcctctc gccgcccgtt atcagatgtc ggtggtgccc tcggcgacgc
     2701 tgtggctcgg ctggcggcag cgccgtcccg gggccgcgcc caatcctgcc ctggcgctgg
     2761 ccgaccctcc cccgttgtca ggtgaagaag cgggagagga ggcggaggcc gcgccggcgg
     2821 ccgagcgctc cgcggtcttc gcctcggcca tgcgcctggg gagcctcccc tatgcccgcc
     2881 gggagagccg ggccgtggtg cgggccatgg gcggcggcag cgtccggcgg ctggggaagg
     2941 acgcgtccga gacgttcttc aagcgcgccg acctggcgcg gttcgggctc atccacctcg
     3001 cgacccacgc ggtggtggac gagcagaacc ccgagcgctc gggcgtgctc ctcgccgcgg
     3061 ccggcggcga cgacggcctc ctccagctcc gggagatcgt cgacctcccc ctggacggcc
     3121 ggatcgtggt cctcgcctcg tgccgtagcg cctcgggaac gctgctccgc ggcgaagggg
     3181 tgatgggtat ggcgcgcgcc ttcttccagg ccggctccca cgccgtggtg gcgagcctct
     3241 ggccgctacg cgacgacgag gccgcgctgc tgttcgaccg cgtctaccac tacctgggcc
     3301 gcggcgaccg cctggccgcg gcgctccagg cggcccagcg cgacctcatc gccgacggcg
     3361 tccccgccgc cgcctgggcc ggcctggtgg tgctgggcga cggcgacctg gtgccgctgc
     3421 ccggaggccg caagctcccc tacttgagcc ccgccgagaa ggccctcgcc gccgcggtcc
     3481 tcctggcgct gctcggcgcc ggagccctgg cctggtaccg ccgccggcgt acgcgacgcg
     3541 gtgacgtcca taccgtcccg tcgccgagcg tctagaagac gacgagccgg cgcaatctcc
     3601 ctcaagccgt tgccagctcc gccgcgccgg cgaagatggc aacgctccgt cgcggtccgc
     3661 agcccgccgg cgcagtccgc gacgcctcgt tgcagtctcc atcacgccgt tgcaagcttc
     3721 cgatccgtca gggaagagcg caacaccctg ttgcactcat cgccgggccg ttgcaagctc
     3781 ccgccgcgcc ggcgcggagc gcagcgtgcc attgcagcct ccgccaggcc gttgcagcct
     3841 gccaacgcgc gggcgctgtc tgcgacgagc cgccgtacac cgcgccaagc cgttgcaagc
     3901 ttccaatctg ccgccggagc ttgcaacgcg ccgtcgccat cttcgacaag agaaaagcgc
     3961 ctccccctct cccgaggagg ggggaggcga ggcggaagag gggccagggg cgaggcctac
     4021 gcgcccttcg ccagcttgcg ggtccccccc accgcgcggc gccgctcctt gcgggcggcg
     4081 gtggcgaccg tcgcggtgac agtggtagtg accgccgcga cggcagcggc cgtgggcacg
     4141 gccagcgtcg tgcgcgacgc ctcggcccgc ttctggccga cgatgcgggc atagaccgat
     4201 agcgcctgcg ccaccacctg gtccatgttg tagtacttgt acgtggcgag ccgccccacg
     4261 aagtggacgt cctgcgtgga ctcggccagc gcctggtatt tcttgtacag ctcctggttc
     4321 tccggccgcg gcaccggata gtaggggtcc ccctccgcca tcgggtactc gtagaccagg
     4381 gtgctcttct tgtgctcctg ccccgtcagg tgcttgaact cggtcactcg cgtgtagagg
     4441 tgctcgttgg gatagttcac caccgccacc ggctgcaccc actcctggtt caccgtctcg
     4501 aacttgaagt cgagagaccg gtacggcagc gggccgtaac ggaaatcgaa gaactcgtcc
     4561 accgggccgg tgtagaccag cttgtcgtaa tccacctcct cgacgatctc gcgatagtca
     4621 caattgagga ggatcttgat gttctcgtga tcgagcatgt tctcgaacat ccgcgtatag
     4681 ccatggagcg gcatggcctg gtaggtgtcc gagaagtagc ggtcgtcgcg gttggtgcgc
     4741 accggcacgc gggcggtcac catggagtcg agctgcgacg ggtcgagccc ccactgcttg
     4801 cgggtgtagc cgcggaagaa cttctcgtac agctcgcggc cgacgcggga gaccacgacg
     4861 tcctccgagg tacggacgtt ctcgcgcggc tcggcgaccg actcgaagaa cttctccacc
     4921 tccatgctgt tgagcttcag gccgtagagc tggttgatgg tgtcgaggtt gatcgggatc
     4981 ggcacgagct ggccgtcgac gctggcgcgc acccggtgct cgtaggggcg ccattcggtg
     5041 aactgcgaga ggtactcgaa gacctcgcgc gagttggtgt ggaagatgtg aggcccgtac
     5101 tggtggatca ggacgccggc gctgtcatgc cggtcatagg cattgccccc gatgtggggg
     5161 cgccggtcga cgagcaggac ccgcttcccg gcgccgcgcg ccagccgttc ggccagcacg
     5221 ctcccggcga agcccgctcc cacgattaaa taatcgaaca tggaccgctc ctttcaagga
     5281 agtcatctct tcaagcaagc atcggacgcg gttcgccgct ccggacaaaa aagaacatcg
     5341 caaaaagcgt gctaaggagc tcaggccacc cgccgccgcg cggcgaccgc cgactcgatc
     5401 agctcggaca tgcgctgcca ggtgctgtgc caggagctcc cggcgaggaa gccgtcgacc
     5461 tgccgtagcc actcctcgcg tgcctcgccg gcccccctgc ccatatccag ggcgcgttcg
     5521 gccgccgcga cgaatccggg cacgtcctcg gcgatctcca ccaagcccat ctcgccgtac
     5581 gggcgcacca catcgcggat gggggtcgag accaccggcc gcccccccgc caggtactcc
     5641 ggcgtcttgg tcgggctgat gaagcgcgtc gactcgttgc gggcgaacgg catcagggcc
     5701 acgtcccagc ccgccaggta gtccggcagc tcctcgtact tcttcatgcc cgggtagtgg
     5761 agattggcgc ggcgcggcag gctcgcaggg tcgatcttga ccaccggccc caggatcacc
     5821 aggtgccagt ccggccgggc gtcggcgacg ccggccagga tgtcgaggtc gaggcgctcg
     5881 tcgatgacgc cgaagtagcc caggcggggc cgggggatgc cggcctggtc cgcgggctcg
     5941 ctgccgcgga gctggcgggc gcggccgaag tgctcggcgt cgatgctgct ggggaaggcg
     6001 tggacgtccg ggtggcggtc gcgcttggcc tcgtagaggc tctggccgcc ggtgaagacc
     6061 aggtcggcgg actcgaggag acgcgcctcc cgctccagca gctcgggcgg agcgccgcgg
     6121 aacagggaga gctcgtccat acagtcgtag accacggcgc tcggcgccaa gccggcggtg
     6181 aaggccagcg ccatcggggt gtagtaccag aggatgtagt cggcgatgcc ctgctcggcg
     6241 aacaggctgg gcaggagctc gcgctgcgcc gcctccgccg cctgggcatc caggccgtgg
     6301 ggcaggcgcg gcacgacgac gcgcacccct tccggccggt cgctcacctc caggcggggt
     6361 gggccgtcat gcggcatcgg ctcctcgaca aagaacaccc gccggtcgcg ggcgaaacgg
     6421 ctcatcagat gctgcggccg ctggtagacg aagtcccagc gcagatgcga caggcagacg
     6481 aggtccggct tccggtcccc tgcctgatca gaaaacggca tgctcgatct cctcctcggt
     6541 cgtagccagt tgaacctctg cagtcccctc ctcgcgccgg aacagctccg tctgcagctc
     6601 cagctccatc gccagggacc cgtgctcctc ccgctcccct tcatcgccgc agaagcccca
     6661 gagcccggcg gggcagtggc ggtcatcgtc ccagcccgga tagtcgagga tgggatagag
     6721 gcagatgcca tggacgggga ccccggcacg gcgggccgcc cgcacctcct cgctgacgta
     6781 gcgcagccac ggcgcccggt cctccccctc ggcgccggtc tcggtgatca gcatgggccg
     6841 gccgaagcgc tcccagatct cctgcacgat cgcgcggaaa ggccggtaga gaggctcccc
     6901 gcgctggatc ttgccgccgt tcaggaccca ctggttgccg gggtagtaat tgacgcccag
     6961 cacgtccagg tagggggcgg cgccgccgag ttggggccag atgccgccgc gcagcatctc
     7021 ccagccctgg aactggcaca gccgctgctc ctcggccgcc gccgcctcct cggggcggcg
     7081 cggatcggcc gcgatgtgga tggccggatc ggcatgaacg atccgggctc ggggatccac
     7141 ctcccacacg gcatcgatgg cggccagggt tgcccgcacg agttggacct tgagctcgaa
     7201 gccccgcccg cgacagaagg gattcagata ctccacgtcg ccgccggccc aggacaggaa
     7261 cgagatctcg ttgaccggcg cccagagggg cggctcaccc gtctcttcgc ggacgacccg
     7321 ggccacctcc cgggcatagg ccgcgaagcg gtcggcgaac cgggccccga agacgtccag
     7381 gtcgtcgggc cagccgtagt ggcagaggtc ccagatcact tggatcccca ggtcccgcgc
     7441 cgtccgcacc atggggagca gagaggagaa gtcgtagcgg ccgggcgacg gctcgatcag
     7501 gtgccagcgc accccctcgc gggcggtggt catccccaga cgccgcagcc gttcatagtc
     7561 ggccgcgagc catcggtcat gcccagtggc ctcgatcagg tcgaggcgcc ggccgtcacg
     7621 tcgccggtga caggcacact cgaatcctcc gatccagaaa ctcttcgtca gcattcaatc
     7681 cttggtcgat ccggagcggg ctcacgcacg caccgctccc ggctgcaaga tgcggaccat
     7741 ggccggcctc ctccccgccg cccccctctc ctccccgtgg cggtgcggct gcgagaacac
     7801 gaaaatggat ttcctgccat gggagtgtca ctttttttgc ccttgaagca actttaaggt
     7861 gggggccgat ctcgccttcc ccgcggacag agaggaagag atgacagagg agggggttcg
     7921 atgagcgcac actaccatcc cgaccggagc gcgctggagc ggttcgcccg aggcaggtcc
     7981 tccgccgtgg aggaggcccg catcgaatcc catttgcgca ccggctgcgg cctctgccag
     8041 gccgaggtgg accggctgct gccggccgac ccagaggtct ctaccggacg cccggccggc
     8101 gcgaaggagc gcgtgaagat ggcggcgggg ctcgaggagt cgggctggga gcgcgttctc
     8161 ggagagctcg agcggcggct ggcctcggtg atccaggaga gggaggccgc gccgcaacgg
     8221 gtggcggaga tcctcggccg tcccaccgag gcacggcacg agctgatcgc gtccgaccgg
     8281 cgcttccact ccctcgccgt ctgcgagctg ctcatcgacc agagcttcga cgaggggttc
     8341 caggatccgg cgcgggcgct gcagacggcg gagctcgcgg tggcggtggc cacccggctg
     8401 gagacggggc actacggctg ttccgtggtc cacgacctgc aggcgcgggc ctgggcctac
     8461 ctgggcaacg cctgccggat ccgcggcgac ttcgccgagg cggagcgggc gctggccagg
     8521 gccgaggcgc tcgccgaggc gggctccgcc gaccccctgg aggaggcgcg catcctcgac
     8581 ctccgcgcct ccctcctcgc cgaccagggt tggctggagg aggcctcgga cctcctcgac
     8641 gtggtcatcg acatctgtga cgagatccgc gacctgcacc gcaaggggcg agccctgctc
     8701 agcaagggga tcctgcaggg agacgccggg gagccggaac gggcggtgga gctgatcacc
     8761 gccgggctgt ccctgatccg ctgggaggag gagccgcgcc tcgtgctggt cgcgcggcac
     8821 aacctgatcg cggctctcgt cgcctgcggc cggtgcgagc aggcacggct gcagctcgag
     8881 cacctccgcc tcagcgttcg tgacgcctcc agctcctgga tggagcccca cctcctctgg
     8941 atcgaggggc gcctcgccgc ccggcaggac cggttggacg aggccgaggc cctgctgctg
     9001 gaggttcagc gcctcctcct cgagcaggga cgggactacg acgccaccct ggtgatcctc
     9061 gacctggcga aggtctacct gcggcagggc aagcacctcg acatccgccg gctcgccgac
     9121 cacatgctcc cgctcctgct caaccacgac ggctgccacc aggcgatcac cgccctcgtg
     9181 accttccagc aggcggccga gatggaccgg ctcactccgc acctgatcca ggggatcgcc
     9241 tcctacctgc tgcgtgcccg caagaacccg aagctgcctt ttcacgaggc cgccacggct
     9301 tgagccccgt accggcattt cttaccggca cgagtgtcaa aagttcccac gccacgcggg
     9361 cctccctacc ttcgggcggc cgcgatatcc tcacgcgcca tggctactcc ccttcccctg
     9421 gctccggtag tgccggcggc cacggcccgc cgcctcctga tggacgccca ggagctgctg
     9481 gccgatcccc ggcggcgggc gacggccggc accctgtacg agatcatcga gcggatgggc
     9541 ttcgtccaga tcgactccat caacgtggtg gagcgggccc atcacctgac gctggcggcg
     9601 cggctccagg gctaccggcc ggcgctcctc gaccgcctgc tggagcgcga gcggcggctg
     9661 ttcgagcact ggacccacga cgcctcggcc atccccagcc tctggtaccc ctactggcgc
     9721 caccgcttcg agcgctaccg ggagaaggtc ctccaccacc cctggtggcg ggagcgggtg
     9781 gggcccgatc ccgagtccat ggccgagcag gtccggcagc ggatcgcccg cgaggggccg
     9841 gtgatgaccc gcgacttcga ggacacccgc ggcgaggggg cggagaagac ctggtggggg
     9901 tggaagccgg agaaggcggt gctcgagtac ctctggcgca ccggcgagct cgccatcgcc
     9961 ggccgggtca atttccacaa ggtctacgac ctggccgagc gggtcttccc ccacctccac
    10021 accgccccgc ggcccacgga cgcggagcac ctcgactggg cctgccgcac cgccctggag
    10081 cggctgggcg tggccacggc cgacgagatc gcgggcttct ggcgcgccgt ctccctggac
    10141 gaggcgcgga gttggggccg ggaggccgcg gcgcgggggg agatcgccgc cgtgcaggtg
    10201 gaggcggtgg acggctcgaa gccgcggagc gcctgggcgg tccccgactg ggcggagcgc
    10261 gccgccgccg cgcccgccgc gccgccgcgg ctccggctgc tcagcccctt cgaccccatc
    10321 ctccgggacc gcaagcggac cctccggctg ttcggcttcg actaccgctt cgaggccttc
    10381 gtgcccgcgc cccagcgccg ctacggctac tacgtcctgc ccatgctcga aggagagagg
    10441 ctggtcgggc ggctggaccc caagctccgg cgcgacgagg ggctgctgga gatcaggaag
    10501 gtctggtggg agccgggggt gcggcagagc cgggggcggc tcgccgccct ggaggcggcg
    10561 gtcgcccgcc tcgccgggct ggtgggggcg gagcgctggg agatcgcggg ctctgactag
    10621 ccgtcgcggc taggtgtcga cgcggcggcg ctccgccgcc cggtcgagcg cccgctgcat
    10681 cagcaggtcc tggctggagc gcgcgtcctc gccgggtgcg ggccggcgct gcacgtagct
    10741 gccgtccggg cgcatgtccc aggccttgac gttgtcggcg agctgcaggt ccaggatcgt
    10801 cttgatctcc tcctgaaggc gcggctcctc caccggcacg atcgcctcca cccgggcgtc
    10861 caggttgcgc gacatccagt cggccgagcc gatgaagtgc tcgggcgcgc cggcgttggc
    10921 gaagtagaag atccgggtgt gctccaggaa gcggccgacg atgctgatga cgcggagggt
    10981 ctcgctacgg cccggcaggc ccggccgcag ccggcagatg ccgcgcacga tcaggtcgat
    11041 ctccaccccg gccgccgacg cctcgtagag cgcctcgatg atctgccggt cgtccagggc
    11101 gttcatcttg gcgatgatgc ggcccccccg gccggccttc tggtgctcca cctcgcgggc
    11161 gatgagctcg aggaagcgcg ggcgcatgtt gatcggcgcg accagcagct tcttgaaacg
    11221 gggctgatag acgtagccgg tcaggagatt gaagagctgc gccacgtcct cggtgatgtc
    11281 ctcacggcag gtgaagaggc cgaggtcggt gtagagcttg gcggtctcgg ggttgtagtt
    11341 gccggtggcg atgtggcagt agcagcgcag ggtggcgtcg cgctcctggc gcaccacgag
    11401 ggcgatcttg gcgtgggtct tcaaccccac caggccgtag gcgacgtgcg cccccacctt
    11461 ctccagcgcc tcggcccact cgatgttggc cgcctcgtcg aagcgcgcct tgatctccac
    11521 cgtgacggcg atctgcttgc gcatctccgc cgcccggatc agcgcctgca tgttgggcga
    11581 ctccgccgaa gtgcggtaga gggtctgctt catggcgaag accgccgggt cgtccgccgc
    11641 cgcctcgatg aagcgctgga cgctggtggc gaacgagtcg taggggtggt ggaccaggaa
    11701 gtcaccggcg gcgatggtgg cgaagatgtc cgtcgcctcg tccggggccg gggggtggag
    11761 gcgcgggtgg gtcaccggcg cccagggccg gtagcgcaac gacggcagcg gcacgctgct
    11821 ggcgagcgtc atcagatcgc gcagcgccag cgggccctcg gcctcgaaga tctcctgcgg
    11881 cacgacctcg agctcctcgg agaggaggtc gagcatccac cccggcatgc cggcggccac
    11941 ctccagccgc accacggtgg cgaagcgcct ctcccgcagc tcctcctgga tcgcctcgag
    12001 caggtcgtcc gccgtctctt cgttcctctg cacgtcggcg ttgcgggtca cccggaaggg
    12061 gtaggactcg acgatttcca tcccggggaa gaggcgatcg aggttgtggg cgatgacctc
    12121 ctccaggggg acgaagcgca gggtcttcgg gatctggatc cagcgcggca gcaggtgcgg
    12181 cgccttgacc cgggcgaagc tcacctcgtc ctcccccggc gccttgaccg acaccgccag
    12241 cgacaggctg aggttggaga tgaacgggaa cgggtgcgcc gggtccaccg ccagcggcgt
    12301 gaggatggcg aagatcttgc ggtcgaactc ctccaccagg tggcgccgct cgctggcctt
    12361 gagctcgtcc caggtcagga tctcgaggcc gtgctggcgc agcgccggca gcagctcctc
    12421 gttgagcagg cgccgctcca gcgcgagcat gggccgcacc gcggcgttga tggccgccag
    12481 ctgctggcgc ggcgagccgc cgtccggcgg caggtcccgc acgttgctgg cgagctgctg
    12541 cttgagcccg cccacccgct tcatgaagaa ctcgtcgagg ttcgagtcga agatggcgac
    12601 gaacttgagc cgctcgagca acgggatgga ggggtcctcc acctcgtgca gcacccgccg
    12661 gttgaactcc agccaggaca gctcgcggtt cagaaacagc ggcaccgcat cctccgcggg
    12721 ctccgccggc cccaggggcc gctcgaacgg caacgtcttc aactcggaca agattcgacc
    12781 tcctctccct cggccatttt cccacaccgg accgccagcg tctgtgacag cagcgtgacc
    12841 gaaattgccc tgccgggcgg gcgggcctag aatgcccacg tggcgacgga tgcgacggcg
    12901 gacctgacag cgcagcctct gaaattcccc ggggccgatc cgagcgggca cgatgccggc
    12961 gccgcgcccc cggccggcga gcgcctggcc gccatcgaca tcggctccaa ctccatccac
    13021 atgatcgtcg tcgccgccga gcccaacggc ggctaccgcg tcctcgggcg cgagcgggag
    13081 atggtccgcc tgggcaagag cggcctgagc gagggcgccc tctccgaggc ggcgatgcgc
    13141 gacggcctcg aggcgctggt caagatgacc accctggcgc gcctcaaggg ggcggagcgg
    13201 gtggtggccg tcgctaccag cgccgtccgc gagtccgcca acggccagga cttcctcgcc
    13261 cgggtcaagg cgcagaccgg cctcgacgtc cagctcctca ccggcgtcga ggaggggggg
    13321 ctcatctacc gggcggtgcg cgaggtggtg gacctggggc ccggcagcgc ggtcatcctc
    13381 gacatcggcg gcggcagcac cgagtggatc accagccgcg ccggcgagtt ggaccgcgtc
    13441 gtcagcctga ccctggggtc gctgcgctgc gccgccgagc tcaaggggga cccgcccacc
    13501 gccggctcca tcgacaggct gcgccggcgc gtccgcgagc gcctcgccga agccgtgccc
    13561 ggcatccagg tggagcgcct ggtggccacc tccggcaccg cggtctgctg cgccgacctg
    13621 gtggacctct tcgcctcccg cccctggaag gagcagtccg gcgccctgcg cgaagtgcgc
    13681 gtcaaggact tggcggcgct ggtcgagcgc ctgcgcctgc tccgccgcgc cgagatcgcc
    13741 aacctgcccc cggttggcgg cccgcgctcg gacagcctcc tcgccggcgc catcctcctt
    13801 caggagcagg ccgcccacgc cggcgtcgag cggttcctgg tctccgaccg ggcgctgcgg
    13861 gaggggctgg tcctcgccgc cctcggccgg ccgatcccga gcgccaccga gcccgaggac
    13921 ctgcggcgcc gccagatcct gcagctcgcc gagcgcaccg agagcgtcct gcggcacaac
    13981 ctgcagagcg cccgcctcgc cgtccgcctg ttcgacgtca ccgcctcgct ccacggactc
    14041 ggccagcggg aacgcgaatg gctggagtac gccgccctgc tccacgacat cggctactcc
    14101 atccactacc gcagccatca caaacacacc ttctatctca tccacaacgc caacctggac
    14161 gccttcgacc agcgcgagat cgagatcatc gcccacgtcg cccgcttcca ccgcggcgcc
    14221 tcccccaagc ccgagcaccc ggacctggcc gacctcaagc cgtggcagca gcggacgatc
    14281 cgccagctcg tcgcgctgat ccgcctggcc gactccctgg accgcaccca cgcttcccgg
    14341 gtggacgaga tctatgccgc tattcgcggg ggtcgctccg gcaagtccgg ccgctcctgc
    14401 cgggtgaccc tggaggtcct ctcgcgctac gacgtcgagc tggagctcga ggccgcccgc
    14461 caccatggcc ggctgttcga gaaggtgttc ggctgcaagc tacgccttcg ccagggcctc
    14521 gaggtggcag ggggctgacc gtctcctttt tacacggttt gtaacaagtt ccttcacgga
    14581 cccgggcggg caagtgctcg gttccaagcc ctaaatggcc gcgtcacaaa gagttaacag
    14641 acctctcacc tcgatttcac ctggcatttt gtggaaatct tcccacctgc tctccccggc
    14701 gcttgcccaa acggggaagc cggcccgtaa acctcctaat atcaatgggt tatctggttt
    14761 gcacaaacct ctgttcaatc cgtggaaagg cccgaaagag cacgtccgga ggcccctttc
    14821 cgagggtttt tccgcaggat tttccgcacg agctgtggaa gacgccgtgt tacgcttcga
    14881 acacatggca cacgaacgga tcctggtcat cgaggacgag ccggacatcg ccgaggtgtt
    14941 gcagtacaac ctcgagaaag agggtttccg ggtggagacg gcgcggcgcg gcgacagcgg
    15001 gctggaggcg gtgcggtcgg acacgcccga cctcatcgtg ctggacctga tgctgcccgg
    15061 cctcgacggg ctggagctgg cgcgggtgct caagcgcgac tccaccacct cccgcatccc
    15121 catcgtgatg ctcaccgccc gcggcgaaga ggtggaccgc atcgtcggcc tggagctggg
    15181 ggcggacgac tacatcagca agccgttcag cccgcgcgag gtggtgctgc gggtcaaggc
    15241 ggtgctgcgc cgccggcagc ccgaggaggc tcccgcctcc gagcgcctgg agctgggcgg
    15301 catcgagctc gacgtggcgg cgcaccagct ccgggtacgc gccaaggaga tccccctgac
    15361 cgccacggag ttccggctgc tgcggctgct catggagcgt tgcggccggg tacagacgcg
    15421 cgggcagctc ctgtccgacg tctgggggta cgcggaggac atcgacagcc gcaccgtcga
    15481 cacccacatc cgccgcctgc ggcgcaagct ggggccggag gccgaccgca tcgagacggt
    15541 gatcggcgtc ggctaccggt tgaggccctg acccccttgc gcctgctggg cttcccccgc
    15601 aagctgcggc tggcgccgtt ctggccggcc ctggccgcgg tgctgaccgc gagcctgctg
    15661 ctctggcagc tcctgccggg cctcttcgag cgcggcgcct cggcccagct cctcgacacg
    15721 ctgcagatcc tcacccgcct ggtgtcccag cggctcacgg cgagcgggga ggacgtcgac
    15781 ggtcccgtgg cggccgatct ggaggcgtgg gtccgggaca tcaccgccga gagcgacctg
    15841 cgcatcaccc tcatccgcgc cgacggtcac gtcatcgccg acagctcgcg cacggtcgag
    15901 caggtgcccg gcatggagaa ccacgccggc cggcccgagc tccaggaggc gttgcgccgc
    15961 ggacagggtg tggcggtacg gcggagcgag accaccggcc tcacctatgt ctatgccgcg
    16021 cgcacgctga ccgtgccccg tgccggcggc gccccaggtg agagcgggtt gctggtcctg
    16081 cgcctcgccc agccgctggc ccaggtcgaa gcgctgcgcg gcgggctcgc cgcggccatg
    16141 ggactcgcgg cggcggcggc cggcctggtg atcttcgtcg tctcctggtg gatcggccgc
    16201 cggctcttcg acccgctggc ccggctcatc gtcggcgcgg gtgacctggc ctccggccgc
    16261 aaggagcagg tcgaggtgcc ggacgaggag gagctggccc ccctggcgcg ggcgctcaac
    16321 cacctcgccg agaccgccgc ggcgcagttc gcggcggtgc gccaggagcg cgaccacctc
    16381 atggccatcc tcgccagcat gtctgaaggg gtgctggtgg tggacggcga gggccgggcg
    16441 ctgctggtca atccggcctt ccggcggctg ttcgacctcg cgggcgaggt caccggccgg
    16501 ccggtcctgg agatcgtccg cctccccgag ctggcccggg tcgtcgcgga cacgctacgg
    16561 ctcggcgagg ggcagggggg gcaggtcgag ctggccgcgc cggagaagcg cacgctcctc
    16621 ctggcgagcg ccgccctctc cggcggcggg cgcggcgcgg tggtggtggc ccgcgacacc
    16681 accgagctga cgcgcctggg ggacatgcgg cgcgacttcg tcgccaacgt ctcgcacgag
    16741 ctcaagacgc cgctcgccgc catccgcggc tacgccgaga cgctgcgcga cggcgccctc
    16801 gacgagcccc ccatcgcccg ccgcttcact gagcgcatcc tctggcagtg ccggcgcctg
    16861 caggccctgc tcgacgacct gctgaccctg tcgcgcctgg agagcgtcac cattccgcgc
    16921 gagcgggagc cggtggacct ccacgccgtg gtccgccgca tggccgagct gctgtcggcg
    16981 gcggcgcggg agaagcaggt gcggatcgag gtggaagaga agcctgtgcc gccggtcctc
    17041 ggcgacgccg acggcatcga gcggatgctc tcgaacctgc tggacaacgc catcaagtac
    17101 aaccgtcccg acggccgggt gacggtccg
//
LOCUS       DASPJI010000681         5716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_611143, whole genome shotgun sequence.
ACCESSION   DASPJI010000681 DASPJI010000000
VERSION     DASPJI010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611143"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2217)
                     /locus_tag="VEL74_19245"
     CDS             complement(<1..2217)
                     /locus_tag="VEL74_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019486948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYG64725.1"
                     /translation="MSTHDLENAVAIVGMAGRFPGAHTLEQFWHNLRNGVDSVRSLSD
                     EEIVAMGVSPAMLADPGYVRATAQPDGIDLFDANFFGINAREAEILDPQQRVMLEVAW
                     EALENAGYDPGGAGRVVGVFAGQTLSTYLLFNLMPDAGLRSVMDPLQLLVGNTGDSLA
                     TRVSYKLNLQGPSYTVQSACSTSLVAVHNACQSLLAGECDMALAGGVSLNVNLLGGYL
                     RKEGSVFSPDGRCRAFDARAQGILFGGGAGLVVLKRLEDAIADGDTVRAVILGSAVNN
                     DGGLKAGYTAPSVEGQAGVIAEALGAAGVEAESISYIEAHGTGTRLGDPIEIQALTKA
                     FRDETDRRGFIPMGSVKTNIGHLDVAAGISGLIKTVLAMEHREIPPSLHFHEANPEID
                     FAASPVFINTELRPWQPAPAAPCRAGLSAFGFGGTNVHMILQEAPQLPARKRAEGAWH
                     LLPLSAKSESALAAASANLAAHLAAHPGLDLADVAHTLQVGRRPMELRRVVACRNDSN
                     GLGEAVAALRSPGAEPVTNVAALDEASRSWLAGQPVDWASLPGVGGRRVPLPTYPFER
                     QSYWIATAGAPRPLSAVKSPEVAPMTSALAPEPEAAVAEIAADPMLAMAAAPAQTFHP
                     RPNLFNPYVPPSTEAEGKVVAIWQEVLGVEPVGVHDNFFQLGGHSLLATQLMSRLRAA
                     CGVELPLRALFEAPTVAALAARVAAAQSERSGLEAPPMLPAPRDGAVPLSFGQQRLW"
     gene            complement(2264..>5716)
                     /locus_tag="VEL74_19250"
     CDS             complement(2264..>5716)
                     /locus_tag="VEL74_19250"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYG64726.1"
                     /translation="DFASSPFFVNDRLREWQTEGIPRRAGVSSLGMGGTNTHMVLEEA
                     PALEPSGPSRPAQLLLLSARTATALDALTRDLAEHLKQHPGLPLADAAYTLHTGRKVM
                     AHRRAVVCADLEEAVDALESRDPERVTDGFREAGARPVAFLFSGQGSQYAGMGRGLYE
                     SEPVFREEVDRCAELLRPHLGLDLREVILAEDEEEAAARLRQTALTQPALFVVEHALA
                     RLWMSWGVRPQAMLGHSIGEYVAACLAGVLPLEDALALVAERGRLMQSMPAGSMLAVP
                     LPEPDVAPLLSMEIWLAAVNRPEVCVLSGTDEAIAALERELEGRGIEARRLHTSHAFH
                     SAMMDPILPAFEAAVRKVTLRAPRLPYLSNVTGTWITAAEATDPGYWVRQLRGTVRFA
                     AGVGELLADPDRVLLEVGPGNALATAARQHPARRPEQAVAVSMRHPKEPKDDLEVLLA
                     AVGRLWLAGVEVDGDAFWAGQRRRRVPLPTYPFERKRFWVDPKKPSFEKTARSSADLA
                     DWFYVPFWKQSQALFARDKVRELGFARGERWLVLLDNQGFGERLLQRLGRAGADVIAV
                     RAGAGTEDPTGVHLQVDPGNDEETTRFFANLREQGWMPSRIVYLWALDSNGPNPRSMD
                     LCLHGLLSVARGLGRPLGEPEQGDLTVFLALTSGAQNITGEEPPSMERAVLTGACKAI
                     PREYSNLYCRSLDIPAPAALKPELQLDLVERVLAEALTAPGDPAATVAGLRGPHRWVQ
                     AFQPVRLDEGAGTPRLREGGIYLITGGMGGIGLTVAEHLARKYRAKLMLSGRTPLPPR
                     PEWEGYLASCAPDDRVGRRLRKLLELKALGAEVMVVTGDAADPQNLRAIRDRAMARFG
                     RIDGIFHAAGLPGGGLIHTRDRRAALSVLGPKIDGTMAVMDTFTDVQPQFLVLFSSVT
                     SLLSQPGQADYAAGNAFLDAFAQATMAGPVFTVSINWDAWQEVGMAVETEVPAELRAW
                     REESLRQGVSPAQGIEALERILRASLPQVAVSRSDFQARVEESHAWKTFQALEESGEE
                     SREVHARPALGSVYVAPRDETERRIAEIWQEVLGIDRVGIHDNFFDLGGNSLVGLKVV
                     SRLKRELAADVSAVTLFEGPTVASLAQLLAGAGEEGEDGEPVYADRKSRGALRREKLR
                     RRKS"
BASE COUNT          822 a         2119 c         2014 g          761 t
ORIGIN      
        1 ccagagccgc tgctgtccga atgatagcgg caccgcgccg tcccgcgggg ccggcagcat
       61 cggcggcgcc tcgaggcccg agcgctccga ctgcgccgcg gccacacgcg ccgcgagcgc
      121 cgccacggtc ggcgcctcga agagggcgcg cagcggcagc tcgactccgc atgccgcacg
      181 caggcgcgac atgagctgcg tcgccagcag cgagtggccg ccgagctgga agaagttgtc
      241 gtggacgccc accggctcga cgccgaggac ctcctgccag atcgccacca ccttgccctc
      301 ggcctccgtc gacggcggca cgtatgggtt gaacaggttg ggccggggat ggaacgtctg
      361 cgccggcgcc gccgccatgg ccagcatggg atcggcggcg atttccgcga ccgccgcctc
      421 gggctccggc gccaaagccg aggtcatcgg agccacttcc ggggacttca cggcggacag
      481 cggccgcggt gcccccgcgg tggcgatcca gtagctctgg cgctcgaacg gataggtggg
      541 tagcggcacg cgccggccgc ccacccccgg caaggaggcc cagtccaccg gctggcccgc
      601 cagccagctc cgcgaggcct cgtccagggc cgcgacgttc gtcaccggct ccgcgccggg
      661 cgaacgcaga gcagcgacgg cctcgcccaa accgttggag tcattgcggc aggcgacgac
      721 gcgccggagc tccatcggcc ggcgccccac ctggagggtg tgggccacgt cggccaggtc
      781 gagccccggg tgagcggcga gatgggcggc gaggttggcg ctcgcggcgg ccagggcgct
      841 ctcgctcttg gccgacagcg gcagcaggtg ccaggcgccc tcggcccgct ttctggccgg
      901 gagctgcggc gcctcctgca ggatcatgtg cacgttggtg ccgccgaagc cgaacgcgct
      961 caggccggcg cggcacggcg cggccggggc cggctgccag gggcgcagct cggtgttgat
     1021 gaagaccggg ctggcggcga agtcgatctc cgggttggcc tcgtggaagt ggaggctcgg
     1081 cgggatctcc cggtgctcca tggcgagcac cgtcttgatc aggccggaga tgccggcggc
     1141 cacgtcgaga tggccgatat tggtcttcac cgagcccatg gggatgaagc cccggcggtc
     1201 ggtctcgtcg cggaacgcct tggtcagcgc ctggatctcg atcgggtcgc ccagccgcgt
     1261 gcccgtgccg tgcgcctcga tgtaggagat gctctccgcc tccacacccg cggcgcccaa
     1321 cgcctcggcg atgaccccgg cctgcccctc gacgctcggc gccgtgtagc ccgccttcaa
     1381 gccaccgtcg ttgttgaccg ccgagcccag gatcacggcg cgcaccgtgt cgccgtcggc
     1441 gatggcgtcc tccagccgct tcaacaccac gagccccgcg ccgccgccga agaggatgcc
     1501 ctgcgccctg gcgtcgaagg cgcggcagcg gccgtcggga gaaaagaccg agccctcctt
     1561 gcgcaggtag ccgccgagca ggttgacgtt cagcgacacg cccccggcca gcgccatgtc
     1621 gcactccccc gccagcaggc tctgacaggc attgtggacc gccaccagcg acgtcgagca
     1681 ggcgctctgc accgtgtagc tcggcccctg gaggttgagc ttgtacgaga cgcgcgtggc
     1741 cagcgagtcg ccggtgttgc ccaccaggag ctgcagcggg tccatcaccg accgcaagcc
     1801 ggcatccggc atgaggttga agagcagata ggtgctgagc gtctggccgg cgaacacgcc
     1861 gacgacccgg ccggcgccgc cggggtcgta gccggcgttc tccagcgcct cccaggccac
     1921 ctccagcatc acccgctgct gcgggtcgag gatctcggcc tcgcgggcgt tgatgccgaa
     1981 aaagttggcg tcgaacaggt cgatgccgtc cggctgtgcc gtggcccgga catagccggg
     2041 atcggccagc atggccgggg acacgcccat ggcgacgatc tcctcgtcgc tcagggagcg
     2101 caccgagtcc acgccgttac gcaggttgtg ccagaactgc tccagggtat gggcaccggg
     2161 gaacctcccc gccatgccga caatcgcgac cgcgttctcc aggtcgtgcg tgctcacgag
     2221 atactcctcc agctccggat cagaaaaaag caaagaaaaa agatcagctc ttgcgccggc
     2281 gcagcttctc gcgccgcaga gcgccacggc tcttgcggtc cgcgtagaca ggctcgccgt
     2341 cctcgccctc ttccccggcg cccgccagga gctgcgccag ggaagccacg gtcggcccct
     2401 cgaacagggt gaccgccgag acgtcggcgg ccagctcccg cttgaggcgc gagacgacct
     2461 tgaggccgac cagggagttg cccccgaggt cgaagaaatt gtcgtggatg cccacccggt
     2521 cgatgcccag gacctcttgc cagatctcgg cgatccggcg ctcggtctca tcgcggggcg
     2581 ccacgtagac gctccccaga gccggccggg cgtgtacctc ccggctctcc tccccactct
     2641 cctccaaggc ctggaacgtt ttccaggcgt gactctcctc aacccgcgcc tggaaatcgc
     2701 tgcgcgacac ggccacctgc ggcagcgaag cgcgcaggat gcgttccagc gcctcgatcc
     2761 cctgggccgg cgagacaccc tggcgcaggc tctcctcgcg ccaggcccgc agctcggccg
     2821 ggacctcggt ctccaccgcc atgcccacct cctgccaggc gtcccagttg atggagacgg
     2881 tgaacaccgg cccggccatg gtggcctggg cgaaggcgtc caggaaggcg ttgcccgcgg
     2941 catagtccgc ctgccccggc tgcgacagga gggaggtcac cgacgagaac agcaccagga
     3001 actgcggctg cacgtcggtg aaggtgtcca tcaccgccat ggtgccatcg atcttcggcc
     3061 ccaggaccga gagcgcggcc cggcggtcgc gggtatggat cagcccgccg ccgggcaggc
     3121 ccgcggcgtg gaagatgccg tcgatgcggc cgaagcgcgc catcgcccgg tcgcggatgg
     3181 cgcgcaggtt ctgcggatcg gcggcgtcgc cggtgaccac catcacctcg gctcccagcg
     3241 ccttgagctc cagcagcttc cgcagccgcc ggccgacgcg gtcgtccggg gcgcagctcg
     3301 ccaggtagcc ctcccactcg gggcggggcg gcagcggcgt gcggccggag agcatcagct
     3361 tcgcccggta cttgcgcgcc aggtgctcgg ccacggtcag gccgatgccg cccatgccgc
     3421 cggtaatcag gtagatcccg ccctcgcgca gccgcggcgt gcccgccccc tcgtcgagcc
     3481 gcacgggttg gaaggcctgc acccagcggt gcgggccgcg cagtcccgcc accgtggcgg
     3541 cgggatcgcc gggagcggtg agggcctcgg cgaggacacg ctccaccaga tcgagctgca
     3601 gctccggctt gagcgccgcc ggggccggga tatcgaggct gcggcagtag aggttcgagt
     3661 actcgcgcgg gatcgccttg cacgcgccgg tgagcaccgc ccgctccatc gagggcggct
     3721 cctcgccggt gatgttctgc gcgccgctgg tcagcgccag gaacacggtg agatcgccct
     3781 gctccggctc gcccagcggc cgtcccagcc cccgcgccac cgacagcagc ccgtgcaggc
     3841 agaggtccat ggagcgcggg ttggggccgt tcgagtccag ggcccagaga tagacgatgc
     3901 ggctcggcat ccacccctgc tcgcgcaggt tggcgaagaa gcgggtcgtc tcctcgtcgt
     3961 tgccggggtc cacctggaga tgaacgccgg tggggtcctc cgtgccggcg cccgcgcgga
     4021 cggcgatgac gtcggcgccg gccctcccca gccgctggag caggcgctcg ccgaaaccct
     4081 ggttgtcgag gagcaccagc cagcgctcgc cgcgggcgaa gccgagctcc cgcaccttgt
     4141 cacgggcgaa cagcgcctgg ctctgcttcc agaacggcac gtagaaccag tcggccaggt
     4201 cggccgagct ccgcgccgtc ttctcgaacg acggcttctt gggatcgacc cagaagcgct
     4261 tgcgctcgaa gggatacgtc ggcagcggca cgcgccgccg ccgctggccg gcccagaagg
     4321 catccccgtc cacctccacg cccgccagcc agagccgccc caccgccgcc agcaggacct
     4381 ccaggtcgtc cttcggctcc ttggggtggc gcatggacac cgccaccgcc tgctccggcc
     4441 ggcgcgccgg gtgctgccgc gccgccgtgg ccagcgcgtt gcccggcccc acctccagga
     4501 gcacgcggtc gggatcggcg agcagctcgc ccacgccggc ggcgaagcgc accgtgccgc
     4561 ggagctggcg cacccagtag ccgggatcgg tcgcctcggc cgccgtgatc caggtgccgg
     4621 tgacgttcga caggtagggc agccgcggcg cgcgcagcgt caccttccgc accgccgcct
     4681 cgaaggccgg caggatgggg tccatcatcg ccgagtggaa ggcgtgggag gtgtggaggc
     4741 gccgcgcctc gatgccccgc ccctccagct cccgctccag cgccgcgatg gcttcatcgg
     4801 tgccggagag cacgcagacc tccgggcggt tgaccgccgc gagccagatc tccatggaga
     4861 gcaacggcgc cacgtcgggc tcgggcagcg gcacggccag catcgagccc gccggcatgc
     4921 tctgcatcag gcggccgcgc tcggcaacca gggccagggc gtcctccagc ggcagcacgc
     4981 cggccaggca ggccgccacg tactcgccga tgctgtggcc gagcatcgcc tggggccgca
     5041 cgccccagct catccagagc ctggcgaggg cgtgctcgac cacgaacagg gccggctggg
     5101 tgagcgccgt ctggcgcagc cgcgccgccg cttcctcttc atcctcggcc aggatcactt
     5161 cccggagatc gaggccaagg tgcggccgga gcagctcggc gcagcggtcc acctcttccc
     5221 ggaagaccgg ttcggactcg tagaggccgc gccccatgcc cgcgtactgc gatccctggc
     5281 cgctgaacag gaaggcgacc ggccgcgctc cggcctcgcg gaagccgtca gtcacccgct
     5341 cggggtcgcg cgactcgagg gcgtccaccg cctcctccag gtccgcacag accacggcgc
     5401 ggcgatgggc catcacctta cggccggtgt gcagcgtata ggcagcgtcc gccagcggca
     5461 ggccggggtg ttgtttcaga tgctccgcca ggtcgcgggt caacgcgtcc agggctgtgg
     5521 cggtgcgggc ggagagcagc aggagctgcg ccggccgcga cggccccgag ggctcgagcg
     5581 ccggcgcctc ctccagcacc atgtgggtgt tggtgccgcc catgccgagg gagctgacgc
     5641 cggcgcggcg ggggatcccc tcggtctgcc actcgcgcag ccggtcgttg acgaagaacg
     5701 ggctgctggc gaagtc
//
LOCUS       DASPJI010000682        29665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_341154, whole genome shotgun sequence.
ACCESSION   DASPJI010000682 DASPJI010000000
VERSION     DASPJI010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 29665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29665)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29665
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341154"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1684
                     /locus_tag="VEL74_19255"
     CDS             <1..1684
                     /locus_tag="VEL74_19255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HYG64727.1"
                     /translation="GPEVPVAVLAERSLATVVGLRAIAKAGGAYLPLDPAYPPERLAW
                     MLTDARPRVLLGRPDQLDTLPAMEGGAIVVELTADPDEAGGPEPAGPLSDTVCYVMYT
                     SGSTGRPKGVLISHRALVNRMLGFLSEFSLSDDDVLLQKTAFGFDPSLCELVAPLLAG
                     ARVAMAGPESHRNPAALARDIAGYGITFLQLVPSMLAPLLEEKGLADCRGLRRVLCGG
                     EVLSPALRDAAFARLDAELYNYYGPTECTIDSTFWRCRPGDGSAVVPIGRPVANGQVY
                     MLDRALRPVPPGTVGELYIGGANVARGYLGRPDLTAERFLPDPYSSAPGGRLYRTGDL
                     ARHRFPQGAIEYVGRADLQVKVRGVRIELAEIESALARHPGVLQAVVTARDDGGPVGR
                     RLAAYLVGNAEPAELRRFLAAELPEVMIPAAWVKLDALPLTPNGKVDRAALPAPEGSA
                     GAEVEYVPPSSPLEETLARAAAETLGVERVGMRDNFFDLGGHSLLATQLVSRLTQEHG
                     LDVDLQMVFQAPDLAGLADRIVDRQLAQFEGLEELEGESLEEMLRQMEARVE"
     gene            1681..7311
                     /locus_tag="VEL74_19260"
     CDS             1681..7311
                     /locus_tag="VEL74_19260"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64728.1"
                     /translation="MSEPNDAVSAPRRVEDLSREERLRIFEQLRQRKERQGRPAAEGP
                     GRIPRRPPELDPVPTSFAQERLWFLDRLHPGNPAYNMPMALEARGDLSPAALEAALNE
                     VVRRHEALRTTFAEREGRPVQVISPPESARRLPLVDLAALPAAVRRQEARRLAEEDGW
                     RPFDLLTGPLLRSTLLRLGPAEHALLFNLHHIVADGWSQGVLTGEIAALYGAAREGRR
                     SPLPELAIQYADFAVWQRQRLTGEALDRQLAYWRERLAGAPESLDLPSDRRERPSSGR
                     GGRGARLRFTLGEELTRGLGVLSRRSEATLFMTLLAAFTAMLRRLSGQDDLVVGSAIA
                     NRNRAEIEPLIGFFVNALALRLDVPGDPPFTELLAAARRLSLEAYGHQDLPFERLVQE
                     LRPERQLSRNPLFQVAMALQNAPSGDAELTGVTFSPLEHETPAAVFDLELHGIELGDR
                     LAVELVYQEDLFEAATIRRMAGQLERLLAAVVEDPARRLSELPLLGGPERHQLLREWN
                     DTGAEAPGDMVSLILEQARLRPAALALSRGEERLTYGEMWDRAGELAARLRALGVGPD
                     TLVGLHADRSPAMVTGALAVWLAGGAYVPLDPSYPESRLAFIAAETRMPVVLTEGESH
                     WNQGVKGVSLNGVKGGGASPPGRGAVGSGGGQEGSFSLPATLAYVIYTSGSTGRPKGV
                     QISHAGLANMVAWFARTYRLTPGSRVAQVASTAFDASIGEIWPALASGASLHFADRET
                     ALEPERFLAWLAAEEIRLTFLPTPLAEQLLEAAERGIPPGLVLKTLWTGGDRLRRYPA
                     PSLPFAVVNNYGPTESSMVTTWAPVEPLPDGVDNAPPPAIGRPVANTRLRLLGRYGEA
                     VPPGVHGELAIAGTGLSRGYLGRPDLTAERFVPDPFAGSPGERLYRTGDLVRQSPDGR
                     LDFLGRIDTQLKVRGFRIEAAEVEGTLARHPEVAEAVVTVRGEGEGARLVAYLVHRPA
                     TGEEGAAGGHVAQWQELYDSNYSANYAGRPDEEADDPTFDIRGWNSSYTGGPIPPGEM
                     RAWVEGTVERLLALPHQRVLEVGCGTGLLLYRVAPHTERYRAVDFSAVAIEGIRRQLG
                     RLGLSQVELAQRQADDWSGAEPGEFDLVVLNSVIQYFPGVDYLLRVLEGAVAAVAPGG
                     TIFLGDIRSLPLQEAFATSVEEARADDSTTPEELRQRIARRIADEEELLVDPALFDAL
                     ARQLPAIRNVRVLSKPDTYDNELSRFRYDVILEVGEGSGEVVPPPLGGREGGSGGGQE
                     GGSSSFTPWSAYVNDPLRGKLARRLSSELRQLLRAELPEPMIPSAFVLLDALPLTPSG
                     KVDRAALPDPEPVRAAAAPTAPRNPAEETLVKLWEEVLGIEGVGVQDNFFELGGHSLL
                     ATQLLSRVRSHLGIDLPLQHLFESPTPEALAAIAMERLERQELQETVPVLALPPVTAH
                     GAREAPLSYRQETFWRVQQSHPEGTTYSMPTPLTLRGAIDAAVLERCFQEAIRRHEAL
                     RTRVEVRDGSPVQVIDPVPAFRLPVVDLRGLPEPMRRREANRLRNVEQKRPFDLARGP
                     VLRATLLHLGGEPDEHVLLLNMHHSLSDGWSFGILTRELAILYEAFAAGAPSPLPEQR
                     YQYADFGRWQRDLLARPESQVHLDYWRRRLAGYTPRQVIPTDRPRGAWTYRGDALPWR
                     LPAPLVLRLRELATAEGASLSMALLTGWKLLLHQAGGCDEILVGLVVALRDRPEFENV
                     IGLFLNELALRTDLSGRPTFRELLRQVKRTLLEAFVHQYVPFPIIAGELAPSLDPAGP
                     PLIQARFNLLNIPVIPEGGPAGIAVEQLPVEEFGAKYDLTLHGLEAGDEVALNLVYST
                     DLFDRGRIERLVERYEALLAAAVAAPDAAPDGDI"
     gene            7437..8699
                     /locus_tag="VEL74_19265"
     CDS             7437..8699
                     /locus_tag="VEL74_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYG64729.1"
                     /translation="MNTNTPLMTEDVAEQVRDRVDSVYRTESRRVLATLIRLLGDFDL
                     AEEALHNAFASAMEQWPREGMPANPRSWLVSTGRFKAIDTIRRRVRFDATVAELAQRV
                     EAEEWDGEGLEDDRLRLIFTCCHPALPADARVALTLREVCGLTTEEIARAFLATPPTI
                     AQRIVRAKKKIRDAGIPYQVPSRADLPDRLDTVLDVLYLVFNEGYSASSGDSLTRPDL
                     SGEAIRLGRLLMELLPEPEVLGLLALMVLHESRRAARASPDGDIVLLGDQDRSLWNRE
                     LIAEGLALVERALSSRRFGVYTLQAAISAVHAQAPGTEATDWRQIVGLYDLLARAAPS
                     PVVELNRAVAVAMRDGPAAGLALVDAILERGDLADYHLAHSARADLLRRLGRSEESAA
                     AYRKALSLARQEPERRFLEGRLRELGAG"
     gene            8812..9927
                     /locus_tag="VEL74_19270"
     CDS             8812..9927
                     /locus_tag="VEL74_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HYG64730.1"
                     /translation="MSGVRVLVGTRKGAFVLTADGKRQKWDVSGPHFAGWEIYHVKGS
                     PVDPNRLYASQSSGWFGQLIQRSDDGGKTWEPVGNEFVYDGVPGTHQWYDGTQHPWEF
                     KRVWHLEPSLTDPDTVYAGAEDAAMFRTTDGGRTWHELSGLRQHGSGPRWQPGAGGMC
                     LHTILLDPQNPGRIFIAISSAGAFRSDDGGQSWKPINRGLKSEYIPDPEAEVGHCVHR
                     IAMHPSRPATLFMQKHWDVMRSDDAGESWVEVSGNLPTDFGFPIDVHAHEPETIYVVP
                     IKSDSEHFPPDGKLRVYRSRTGGNEWEALTNGLPQSDCYVNVLRDAMAVDQLDECGIY
                     FGTTGGQVYASADSGDTWAPIVRDLPAVLSVEVQTLP"
     gene            9924..10220
                     /locus_tag="VEL74_19275"
     CDS             9924..10220
                     /locus_tag="VEL74_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HYG64731.1"
                     /translation="MIRVVLPFHLRNLARVDAEVEVPVDGPPTLRAVLDALETRYPVL
                     RGTIREHGTQRRRPFLRFFACEEDISHESPDTPLPAEVATGNEPLYVVGAIAGG"
     gene            10248..10604
                     /locus_tag="VEL74_19280"
     CDS             10248..10604
                     /locus_tag="VEL74_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYG64732.1"
                     /translation="MKYMLLTYGDENALDEGERNQCYQDSVQLTHELHAAGKYLGASP
                     LHSTSMATSVRVREGRKLVTDGPFAETREQLGGYYLIDANDLDEAIAIAARIPMALRG
                     TIEIRPIVELSGLPEK"
     gene            10702..11139
                     /locus_tag="VEL74_19285"
     CDS             10702..11139
                     /locus_tag="VEL74_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYG64733.1"
                     /translation="MKIVPYLNFDGQCEEAFKFYEQCLGGKIMGIMKGEDAPMPEEMA
                     AEWKGRVMHACMQVGDQLLMASDSPPGQHQAPQGTYVSVHIDDPAEGRRVFDVLAENG
                     KVEMPFDKTFWAAGFGMCVDRFGTPWMINCAGDTAGQDCQAQA"
     gene            complement(11766..12494)
                     /locus_tag="VEL74_19290"
     CDS             complement(11766..12494)
                     /locus_tag="VEL74_19290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64734.1"
                     /translation="MVRRHPIHFFVCIFVFAVLAAFPLALQAQCPAGQFIIDLKACQK
                     ASHTSVTPYITYTLVEPGNVAGNCSPAPATEFCPATGWKQAALKVVIPPTCTQANIVV
                     EYEGRPAGWSVHIGDSKTNDGHSGDFGTTEHEAELWVAHEILALAPANMLLPLGIDNP
                     LIRQFMNLTDATLKVVVKDQFVSWGPPHSYVQTPATQNLFAIPDTTVPEGDQRAIYVG
                     LNRVVWGPGDRTGCGARRALLSFN"
     gene            complement(13063..13914)
                     /locus_tag="VEL74_19295"
     CDS             complement(13063..13914)
                     /locus_tag="VEL74_19295"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HYG64735.1"
                     /translation="MVNEIIRGVLARAANEHQVGVVAYCFMSSHYHLLVRVHDAEQLA
                     RFAGYLNSNVAREINRLRGRRGHFWEGRYHSIVVSGEEAAQIDRLVYTLSNGCKEGLV
                     ERLADWPGVHAAQALLDGSPVAGTWFDRTQEYGARRRREACETRSFAETQILELVPLP
                     CWDGLSAEIRRSRIAGLVQQIEAEAAALREASGIEPLGREAIERQNPFERPRNPKKSS
                     APRFHAFTKKVRRGLYEAYASFVAAFREAAEKLRAGDRMAAFPTGSFPPHLPFVRDDS
                     PALSPTR"
     gene            14294..15238
                     /locus_tag="VEL74_19300"
     CDS             14294..15238
                     /locus_tag="VEL74_19300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF040323.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG64736.1"
                     /translation="MSHHACSSLEMTVSSSANPISLDRVFPILAVADLAAAVVYYTDV
                     LGFHEAWRWGDPPARVGVARDRVELQLVQAGTPGAPVGDAVVYCHVRGVDALYAECTT
                     RGADICMPLADRGFGLRDFRVRDLDGNILGFGEPITLTRSSSKSEDTMTRRDGENLSE
                     MLNQHGGALRVATAADIPAIFAVRTSVRENHLDLDGLAERGVTPASVAGMLDEAQART
                     WVVEERGEVVAFSVANAQAGTVFALFVHPKAEGRGYGPALLRAAEEWLFAAGWEVIWL
                     NTGQEPHLRAHRVYRTAGWELVGPADHGDVRYEKRRAT"
     gene            15314..15688
                     /locus_tag="VEL74_19305"
     CDS             15314..15688
                     /locus_tag="VEL74_19305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64737.1"
                     /translation="MKRAIAALFGILCATASFAGTAVVTASSDSGGYELTVTTTPNAE
                     KADVFVGSVVLKNKATGEVLAAPKVIFKAGETANLQIQGEGRTLRLELHSDATKNEAK
                     AVVEYSLADEVVFAPTVILELR"
     gene            15782..16189
                     /locus_tag="VEL74_19310"
     CDS             15782..16189
                     /locus_tag="VEL74_19310"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG64738.1"
                     /translation="MGRLDRDRDGSRARCDGLAERGVTPASVAGMLDEPQARTWVVEE
                     DGEVVAFSMADSRAGTVFALFVHPKAHGRGYGRALLRAAEEWLFAAGWEVIWLNTGQE
                     PHFRAHRIYRAAGWELVGPADHGDMRYEKRRAV"
     gene            16431..17768
                     /locus_tag="VEL74_19315"
     CDS             16431..17768
                     /locus_tag="VEL74_19315"
                     /inference="COORDINATES: protein motif:HMM:NF012535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thaumatin family protein"
                     /protein_id="HYG64739.1"
                     /translation="MRAKLRVGCIGLLLCVILTANAAAEDFLMWQMPGTGISFPVYVY
                     SGGSQVGYIPDSTLQVFVGPYAAGTTSGSYQLYYQAGGSWYACALVLASGSIDASTTC
                     PGAVINPPVSQNGAQSNVYTVGVAASAWPAAVAAPSNPVETDYGQRTITLVNNTKYPM
                     IQIGQSATQNTPIAATIKKNAPFVVSVGTNGLSSAAFYLSSYCTAGSVAECGEPPTVA
                     QCSGEPPKNWVCTGGYFPGQVPYATKIEATILAVSDGVPAGASNVDVSAVDGYNTGVR
                     LYPAAPTYCTFTVPPENSNVLGAGYYSSANALAQVVPVSSSSLSAMCEKSSQLPGPPS
                     KGQTAWNLSKVSSSNAFEGCVSPCTYATANVNQNGVTEQDVNLFCCSGPYNTPQTCGM
                     PAGQLGANTSTYNTSIESSSLFKNVYGYAFGDAGSDYACPPETNFIVEFVSTP"
     gene            18343..20448
                     /locus_tag="VEL74_19320"
     CDS             18343..20448
                     /locus_tag="VEL74_19320"
                     /inference="COORDINATES: protein motif:HMM:NF020683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase-like domain-containing
                     protein"
                     /protein_id="HYG64740.1"
                     /translation="MIPATGSAAIHNILGMQTVHSTVLPSGRLLLVSGSSWRNRNGTQ
                     YYPEYPEPETPTGLFIRDEDPFRNEKLDEYYELVNNAAIYDPVANTFYRVPVPVPEAD
                     PDNAGHFAPNDFFCTGHQHLRDGNVLFTGGTQYYSPFRTGNNTSYIFDWRSELETSWD
                     KVDWRVRPASNDETPWTFSGFMKRGRWYPSLLPLLDGRLAVFSGFVGFDKGFPEMHIF
                     EINSWIEVFDPSAFDPATPERAWKAVDVAQVENSPFRTLINPDFVPTPDVPCDARCIE
                     SNKYDAFKLYPENYLQPDGRIYLTREGDWVSLRTCDAAFMRRTKHTYWATLEDENDGL
                     QVSFSRGPDRVEDVTSYGTTFKDPETGQIHLIGGQPTSPGTLYPLNSANPNHFAGGRG
                     SARLETFTPDNSPAGGHWTLDPDFLGTEPEADRTMHYTVALPTGQILIISGGNYDFYG
                     PVFHPILLTPQYDPKTGKRTGYSQKKMLDALEPRLYHNIALLLPDGRVFVSGGNSARA
                     TITPRNGPPPPYSGAGQPPPDLKQVETDLYFFDDGPIGKGLPGQLTTPTENWTAEIYS
                     PPYLFIDPERRAAITSLAPIEKVGYTPSASIGGKNFTLFHSGVAYQMGLKALPNCAPE
                     DARLALIKLPSATHGWQNGQQFIQLDFEPAEGGIKFTTPDAKASMIPPAYYMLFYTDC
                     HGKPAEAWMVRFDDAASEP"
     gene            complement(20526..22226)
                     /locus_tag="VEL74_19325"
     CDS             complement(20526..22226)
                     /locus_tag="VEL74_19325"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HYG64741.1"
                     /translation="MKPKKLTLAMLLPTTLLVSLPGTLSATTYSLTPAADTFVQQSTP
                     NSNYGSDLNLRIRNVATGQGQHSFLRFNIPALDGNILSATLRLRVAGAISEVGYYHVS
                     MGSPSWSETSLTWNNWSSGTVFSFIGSRTNLAANTFLDLDVTNWITPSPVTFALASSA
                     DITGQRFYSEEEYGAYQPQLILQTDGAVPLECGPTGEDPGDPPDLLNELLCVMKTGVP
                     SANVNYSAQLGPTFNQWANAGSENKPVIAAAVALFDGPIVNGTDYRTWWQDFLDRQAS
                     GSGAGNIWHFKGSELFSNVYDAATMTGVLAAHYWAQSHPADPAAAAIKTKAQDYLRRT
                     WYAYALSASAVDFTSVYNVQGATTSLVQTNNRNCPVLAMASPRSKTDYNQDSKRWLFG
                     TAAGFSTDCYKSPQVRDVVNYLAAQYSAVFGLTSTEKSQLQALVNNTTIPGSLSTVFG
                     AARMRADFHFLLWSDGRRATFYTGPQLNNNIAVNGGKGTVYTAMYDPATRQLDLLFVL
                     AQSTESCLDSGTRRVYVDDLSTPGCGSNHWITLPAANPTYHFVLGPTGWRTCSNLACI
                     "
     gene            complement(22349..23743)
                     /locus_tag="VEL74_19330"
     CDS             complement(22349..23743)
                     /locus_tag="VEL74_19330"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HYG64742.1"
                     /translation="MSRISSRSLALALALCALALPACGQPAANDPGHCLRSGPMLGPA
                     EVDEATVWVQTRYPCKVQVRLWPQGRPEAARLSQEVTTTGDMGIALLRLSGLDFGTRY
                     DYEVYLDGKRVERPYPLTLQTQAYWQYRTDPPSFRAAIGSCAYINDPPFDRPGEPYGK
                     SYEIFESIAAAKPDFMIWLGDNVYYRGPDWLTEAGMRYRWAHDRALPQMQALLGTVHH
                     YAIWDDHDYGPNNADRTYRLREESLRVFRDHFPNPSFGTAEAPGVFTRFEWADVEFFL
                     LDDRYYRSPDDMPDGPGKRMLGKEQLDWLKEALAGSHATFKVVVSGSQMVNPLTFFEA
                     FGNFPAEQKELFDFVRDSRVEGVVFLSGDRHHTELLRRQLPGLYPLYEFTSSPLTSGG
                     SRLEKEADNPARVPGTWVTGGVHNFGLVEVSGKPKERVLTLRTLDTHGKELWKHEIRE
                     GELRFPGNRPDDRH"
     gene            23849..24862
                     /locus_tag="VEL74_19335"
     CDS             23849..24862
                     /locus_tag="VEL74_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HYG64743.1"
                     /translation="MSDSVWYVAVQGQQQGPLSQDEVINGIRSGRFGRDAHVFSQGMD
                     NWAPISSRAEFAQAFAGAASMAPPPPPPRGAGAHDIEYELFGEEMQYVEITLDPGEAC
                     IAEAGAFMFMDPGIQMQTIFGDGSGQEGGGFMGKLLSAGKRMVTGESLFMTMFGNSGG
                     NKQKVAFASPYPGKIIPIDLRRHGGRVLCEKDAFLCAAKGISVGIAFQRRLGAGLFGG
                     EGFILQKLEGDGLAFVHSGGTILSRELAPGETLRVDTGCLVAFESQVTYDIQMVSGIK
                     TAFFGGEGLFYAALTGPGRIWLQSLPFSRLAKKIFGAIPQQKGEGSILGGVGLGALLG
                     GDS"
     gene            complement(24869..25147)
                     /locus_tag="VEL74_19340"
     CDS             complement(24869..25147)
                     /locus_tag="VEL74_19340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64744.1"
                     /translation="MNGPVRGRQSTDPLDFFPDRRVSSQEVRRILRQGTDEERSWVIS
                     HLLRYAQWDDIWAFVSREEIREVFPSLDLPENLRQAWGRMLKIEAPVS"
     gene            25214..25289
                     /locus_tag="VEL74_19345"
     tRNA            25214..25289
                     /locus_tag="VEL74_19345"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25247..25249,aa:Gly,seq:ccc)
     gene            25401..25904
                     /locus_tag="VEL74_19350"
     CDS             25401..25904
                     /locus_tag="VEL74_19350"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HYG64745.1"
                     /translation="MSIFRRDRESTGSAPSSPSPASEAPAASSQRRRITHIAPGTRIR
                     GEVTGSSELLVEGEVEGDIRVDSVVVVGAEGSIQGPINAQVVRIGGRVTGNVSAAERV
                     EVSPAGSVEGDIAASRVVIAEGAFFKGRIEMSGEKNAAGRSGKGPGEPSKAAGEPQKA
                     PATPGKA"
     gene            25906..27018
                     /gene="ald"
                     /locus_tag="VEL74_19355"
     CDS             25906..27018
                     /gene="ald"
                     /locus_tag="VEL74_19355"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HYG64746.1"
                     /translation="MIVGIPKEIKNHEYRVGMTPASVHALIEAGQTVLVEKSAGLGSG
                     ISDDVYVEAGAEIVPDAAAVYGRSDMIIKVKEPIEPEYELLRPGQLLFTYLHLAPLPR
                     LTDVLLDRQVTGVAYETIEDRQGRLPLLTPMSEVAGRMAVVVGASYLLRSYGGRGTLL
                     AGVPGVPPGDVVVIGGGIVGLNSIKMALGLGASVTVLDTNLDRLRYIDDLFHGAVTTL
                     ASNRYNLLAALRRTDLLIGAVLIPGASAPKLVTRDMLKQMKEGAVIVDVAVDQGGCFE
                     TTRPTTHSDPVYVVDGVVHYCVANMPGAVPRTSTFALNNATLPYALALVSKGFERAVK
                     EDPGLRLGVNTYRGHITSKPVAESQDRPYRPLDELL"
     gene            27031..28737
                     /locus_tag="VEL74_19360"
     CDS             27031..28737
                     /locus_tag="VEL74_19360"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYG64747.1"
                     /translation="MLTRRRFLDLTGRAVAGLGAAHVLGPMPLMAAQAAAVPKSVAVP
                     PAALEVIDAFVKRHMEEWGAPGLTLGVAGRDGVIAARGYGLRDRESRTPVSPEDLFQI
                     GSITKSFIGLCALQLHQEGKLDLERPVREYLPWLRIESSYAPITPHHLLTHSSGLPNP
                     LALPREPLWTGHAPGAHFHYCNLGYNILGLLLTQLDGRPLRDMVRARIFEPLGMSASE
                     PVVSQAIRPRLAASYMPLYDDRPFPRRGELTEAPYLEMDNAAGCIASTPRDMAVYMQM
                     LLNRGRGSKGVVVSPESFARFATAAVPAPAFGPTAGYGYGIAVAPTDGRLILRHTGGM
                     VSFSSAMHLDMDAGLGVFASVNARLGGYRPNAVCAYALSVLRAALAGEALPSPPEPDD
                     PFRVPNAADYAGTYTMPDGRALVVAAAGERLEVVYGGRRAALERAAPDAFLTQHPDFI
                     LDPLVFERESDAVTQVIHGGDWYAGTRYTGPKTFDAPAEWKAYAGQYRNENPWEGSAR
                     VDLRQGRLWLGGAELVPLEPGLFRIGEEDHGPDRVRFETVANGRALHAVFSGTEYLRV
                     ET"
     gene            complement(28746..>29665)
                     /locus_tag="VEL74_19365"
     CDS             complement(28746..>29665)
                     /locus_tag="VEL74_19365"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HYG64748.1"
                     /translation="AGERLDRHVAERLGLPRNQVQRWIRDGLVQVNGREARPSTAVAA
                     GDQVTCDRPKLRDDRIVPESGELPVLHEDEHVVVLDKPPGLTVHPGAGRATGTLAHRL
                     LDRYPEMAGVGGPGRPGIVHRLDQGTSGVMAVARTAGAYARLSRAFAERQVEKRYLAL
                     VYGTPSPPAGTIESPIGRHPDRRKEMAVRPGGRPARTDYRTVAARAGIALVEIGLGTG
                     RTHQIRVHMKTAGHPLIGDPVYGEARWKGLPRPVQAPLRDFTRPALHAWRLSFDHPAD
                     GRRLAFEAPVPADLRELWMEATGTPWPEV"
BASE COUNT         4692 a         9988 c        10222 g         4763 t
ORIGIN      
        1 cggcccggag gtgccggtgg cggtcctcgc cgagcgctcg ctcgccacag tggtggggct
       61 ccgcgccatc gccaaggccg gtggcgccta cctgcctctc gaccctgcgt atccgccgga
      121 gaggctcgcc tggatgttga cggatgcccg tccgcgggtg ctcctgggac ggccggatca
      181 gctcgatacc ctgcctgcga tggagggggg agccatagtg gtggagctca ccgccgatcc
      241 ggacgaagcc ggggggcccg agcccgcggg accgctctcg gacaccgtct gctatgtgat
      301 gtatacctcc ggctctaccg gccgccccaa gggcgtcctg atctcccacc gtgcgctcgt
      361 caatcgaatg ctcgggtttc tctccgagtt ttcgctcagc gacgacgacg tgctcctcca
      421 gaagaccgcc ttcggcttcg atccctccct ctgtgagctg gtcgcgccgc tgctggccgg
      481 tgcccgtgtc gccatggccg gcccggagtc ccaccggaac ccggccgcct tggcgcgcga
      541 catcgcaggc tatggaatca cctttctgca actcgttccc tccatgctgg cgccgctcct
      601 cgaggagaag gggctcgcgg actgccgcgg cctgcgacgg gtgctctgcg gcggcgaggt
      661 gctcagcccg gctctccgcg acgcggcctt cgcccgtctc gacgccgagc tgtacaacta
      721 ctacggtccg accgagtgca ccatcgactc caccttctgg cgctgccgtc caggggacgg
      781 ctcggcggtg gtgcccatcg gccggccggt ggccaacggc caggtctaca tgctggaccg
      841 tgcgctgcgg ccggtgcctc cggggacggt gggggagctg tacatcggcg gagcgaacgt
      901 cgcccgaggc tatctcggcc ggcccgatct caccgccgag cgcttcctcc cggacccgta
      961 ctcgtccgcg cccggcgggc ggctctaccg cacgggcgac ctggcgcggc atcgcttccc
     1021 ccagggcgcc atcgagtacg tgggccgcgc cgacctccag gtgaaggtcc ggggcgtgcg
     1081 catcgagctg gcggagatcg agagcgcgct ggcgcgccac cccggggtgc tccaggcggt
     1141 ggtgacggcg cgcgacgacg gcggaccggt ggggcggcgc ctggccgcct acctggtggg
     1201 aaacgccgag cccgcggagc tgcgccggtt cctcgccgcc gagctgccgg aggtgatgat
     1261 cccggcggcc tgggtgaagc ttgacgccct gccgctcacc cccaacggca aggtggaccg
     1321 ggcggcgctg cccgcccctg agggctccgc cggcgcggag gtggagtacg tgcccccctc
     1381 gtcgccgctc gaggagaccc tcgcccgggc cgccgccgag acgctggggg tcgagcgggt
     1441 gggcatgcgg gacaacttct tcgacctggg cggccattcg ctgctcgcca cccagctcgt
     1501 ctcgcggctg acgcaggagc acggcctcga cgtcgacctg cagatggtct tccaggctcc
     1561 cgacctcgcc gggttggcgg accggatcgt cgaccgccag ctcgcccaat tcgaggggct
     1621 ggaggagctg gagggggaga gcctggagga gatgctgcgg cagatggagg cccgagtgga
     1681 atgagtgagc cgaacgatgc cgtctccgcc cctcgccgcg tcgaggacct gtcgcgcgag
     1741 gagcgtctcc ggatcttcga gcagctccgc cagcggaagg agcggcaggg ccgccccgcg
     1801 gccgaaggac ccggccggat cccccgccgg ccgccggagc tggacccggt ccccacctcc
     1861 ttcgcccagg agcggctctg gtttctcgac cgcctccatc cgggcaaccc ggcctacaac
     1921 atgcccatgg ctctcgaggc gcgaggagac ctctcgccgg ccgcgctgga ggccgccctg
     1981 aacgaggtgg tgcggcgcca cgaggccttg cgcaccacct ttgccgaacg ggaggggaga
     2041 ccggtgcagg tcatctcgcc tcccgaaagc gcccggcgcc tgccgctggt cgacctcgcc
     2101 gccctgcccg ccgcggtgcg gcggcaggag gcgcggcgct tggccgagga ggacggctgg
     2161 cgccccttcg atctcctgac ggggccgctg ctgcgctcca ccctgctgcg gctcgggccg
     2221 gcggagcacg ccctgctgtt caacctgcac cacatcgtcg ccgacggctg gtcgcagggc
     2281 gtgttgaccg gggagatcgc ggccctctac ggcgctgcac gggaggggag acggtcgccg
     2341 ctgcccgagc tggcgatcca gtacgccgac ttcgcggtct ggcagcggca acggctgacc
     2401 ggggaggctt tggaccggca gctcgcctac tggcgcgagc gtctggccgg ggcgccggag
     2461 tcgctcgacc tgcccagcga ccggcgggag aggccatcct ccggtcgggg cggccgaggc
     2521 gcccgtctcc gcttcaccct cggcgaggag ctcacccgcg gcctcggggt gctgtcccgg
     2581 cggtccgaag ccactctgtt catgacgctg ctcgccgcct tcaccgcgat gctgcgccgc
     2641 ctctccggcc aggacgacct ggtggtgggc agtgccatcg ccaaccgcaa ccgggccgag
     2701 atcgagccgc tcatcggctt cttcgtcaac gccctggcgc tgcgcctgga cgtgccgggg
     2761 gaccccccgt tcacggagct gctggccgcc gcccggcgcc tgtcgctgga ggcctacggc
     2821 catcaggacc tccccttcga gcggctggtg caggagctgc ggcccgagcg gcagctctcc
     2881 cgcaacccgc tgttccaggt ggcgatggcg ctccagaacg cgccgtccgg agacgcggag
     2941 ctgaccgggg ttaccttctc gcccctggag cacgagacgc cggccgcggt cttcgacctg
     3001 gagctgcacg gcatcgagct gggggaccgg ctggccgtcg agctggtcta ccaggaggac
     3061 ctcttcgagg cggccaccat ccggcgcatg gcggggcagt tggagaggct cctcgccgcc
     3121 gtggtggagg accccgcgcg gcgcctctcc gagctgccgc tcctcggcgg gcccgagcgg
     3181 caccagctcc tgcgggagtg gaacgacacc ggggccgagg cgcccgggga catggtctcc
     3241 ctgatcctgg agcaggcccg cctccggccc gccgccctgg cgctctcccg cggagaagag
     3301 cggctcacct acggcgagat gtgggaccgg gccggggagc tggccgcgcg cctgcgtgcc
     3361 ctcggcgtcg ggccggacac cctggtaggc ctccacgccg accgctcgcc ggccatggtg
     3421 acgggagcgt tggccgtctg gctcgccgga ggcgcctacg tgcccctcga tccctcctat
     3481 cccgagagcc gcctggcctt catcgccgcc gagacgcgga tgccggtggt gctgacggag
     3541 ggggagtccc attggaacca aggagtgaaa ggggtctcct tgaatggggt caagggcggt
     3601 ggtgcctccc cccctgggag gggggcggta gggagtgggg gaggtcagga gggaagtttt
     3661 tctctccccg ccaccctcgc ctacgtcatc tacacctccg gctccaccgg ccggcccaag
     3721 ggggtccaga tctcccacgc cgggctcgcc aacatggtgg cctggttcgc gcggacgtat
     3781 cgcctcactc cgggctcccg ggtggcgcag gtggcctcga cggccttcga cgcgtccatc
     3841 ggcgagatct ggcccgccct ggcctccggg gcgagcctcc acttcgccga ccgggagacg
     3901 gcgctggagc cggagcggtt cctcgcctgg ctggccgcgg aggagatccg cctcaccttc
     3961 ctgccgacgc cgctcgcgga gcagctcctg gaggcggcgg agcgcgggat cccgccgggt
     4021 ctggtcctga agaccctctg gaccggcggc gaccggctgc gccggtatcc cgcaccctct
     4081 ctccccttcg ccgtggtgaa caactacggc cccacggagt ccagcatggt gacgacgtgg
     4141 gccccggtag agccgctgcc ggacggtgtg gacaacgcgc cgccgcccgc catcggccgg
     4201 ccggtggcca atacccgcct gcgcctgctc ggccggtacg gcgaggcggt gccgcccggt
     4261 gtgcacggcg agctggcgat cgccggcacc gggctctccc ggggctacct gggacggccg
     4321 gacctgacgg cggagcgctt cgtgcccgat cccttcgccg gctcgccggg ggagcggctc
     4381 taccgcaccg gcgacctggt gcgccagagc cccgacggcc gcctcgactt cctgggacgg
     4441 atcgacaccc agctcaaggt gcgcggcttc cgcatcgagg cggctgaggt ggagggcacc
     4501 ctggcgcgcc atccggaggt ggccgaggcc gtcgtcacgg tgcggggaga aggggagggg
     4561 gcccgcctcg tcgcctacct ggttcaccgt ccggccacgg gagaggaggg cgcggcgggc
     4621 ggccacgtcg cccagtggca ggagctctac gattccaact attcggccaa ctatgccggt
     4681 agaccggacg aggaggcgga tgatcccacc ttcgacatcc gggggtggaa cagcagctac
     4741 accggcggac cgatcccgcc cggggagatg cgcgcctggg tggaggggac ggtcgagcgg
     4801 ctgctggccc tgccgcacca gcgggtgctc gaggtgggct gcggcaccgg cctgctgctg
     4861 taccgggtgg cgccccacac cgagcgctac cgagccgtcg acttctccgc cgtcgccatc
     4921 gagggcatcc gccgccagct cggccggctc gggctctccc aggtggagct ggcgcagcgc
     4981 caggccgacg actggagcgg cgccgaaccc ggcgaattcg acctcgtggt gctgaactcc
     5041 gtcatccagt acttccccgg ggtggactac ctgctgcgcg tgctggaagg agcggtggcg
     5101 gccgtggccc ccggcggcac gatcttcctg ggggatatcc gcagcctgcc gctccaggag
     5161 gccttcgcca cctccgtgga agaggcccgg gcggacgact cgaccacccc ggaagagctc
     5221 cggcagcgga tcgcccgccg catcgccgat gaggaggagc tgctggtcga tccggccctc
     5281 ttcgacgccc tggcccggca actccctgcc atccggaacg tccgcgtcct ctccaagccc
     5341 gacacctacg acaacgagct gagccgcttc cgctacgacg tcatcctcga ggtgggtgaa
     5401 ggttccgggg aggtggtgcc tccccccctg ggagggaggg agggagggag tgggggaggt
     5461 caggaggggg gttcttcttc ttttaccccc tggtccgcct acgtcaacga tcccctgcgc
     5521 ggcaagctcg cccggcgcct ctcctcggag ctgcgccagc tcctgcgcgc ggagctgccc
     5581 gagccgatga tcccctcggc cttcgtgctg ctcgacgccc tgcccctcac ccccagcggc
     5641 aaggtggacc gcgccgccct gccggacccc gagccggtgc gcgccgcggc ggctcccacc
     5701 gcgccccgca atccggccga ggagacgctg gtgaagctct gggaggaggt cctggggatc
     5761 gagggggtcg gagtccagga caacttcttc gagctcgggg ggcactcgct cctcgcgacg
     5821 cagctcctgt cgcgggtgcg ctcccacctc ggcatcgacc tgccgctcca gcatctgttc
     5881 gagagcccga cgccggaggc cctggccgcc atcgccatgg agcggctgga gcgccaggag
     5941 cttcaggaga cagtgcccgt cctcgccctg cccccggtca ccgcccacgg cgcgcgggag
     6001 gcgccgctgt cctaccggca ggagacgttc tggcgggtgc agcagagcca ccccgaaggc
     6061 acgacctaca gcatgcccac ccccctgacg ctgcgcggcg ccatcgacgc cgccgtgctc
     6121 gagcgctgct tccaggaggc gatccgccgg cacgaggcgc tccgcacccg ggtcgaggtc
     6181 cgggacggct cgccggtcca ggtgatcgat ccggttccgg ctttccggct gccggtggtg
     6241 gacctgcggg ggctgccgga gcccatgcgc cgccgggagg cgaaccggtt gcgcaacgtg
     6301 gagcagaagc ggccgttcga cctcgcgcgc gggccggtgc tgcgcgccac gctgctccat
     6361 ctgggaggag agccggacga gcacgtgctg ctcctcaaca tgcatcactc gctctccgac
     6421 ggctggtcct tcggcatcct gacccgggag ctggccatcc tctacgaggc cttcgccgcg
     6481 ggcgccccct cccccttgcc ggagcagcgc taccagtacg ccgacttcgg ccgttggcag
     6541 cgcgacctgc tggcccggcc cgaaagccag gttcatctcg actactggcg ccgccggctg
     6601 gccggctaca ccccccgcca agtcatcccg acggaccggc cacgcggcgc ctggacctac
     6661 cgcggcgacg ccctgccctg gcggctgccg gcgccgctcg tcctccggct ccgggagctc
     6721 gccaccgccg agggagcctc cctgtccatg gccctcctca ccggctggaa gctcctcctc
     6781 caccaggccg gcggctgcga cgagatcctg gtcggcctgg tggtggcgct gcgcgaccgg
     6841 ccggaattcg agaacgtgat cggcctcttc ctcaacgagc tggccctgcg caccgacctg
     6901 tcgggacgcc ccaccttccg cgagctgctg cggcaggtga agcgcaccct gctcgaagcc
     6961 ttcgtccacc agtacgtgcc gttcccgatc atcgccggtg agttggcccc gtcgctcgat
     7021 cccgcgggcc cgccgctgat ccaggcccgc ttcaacctgc tgaacatccc cgtcataccg
     7081 gagggcgggc cggccggcat cgccgtcgag cagctcccgg tggaggagtt cggcgccaag
     7141 tacgacctca ccctccacgg actcgaggcc ggcgacgaag tcgccctcaa cctggtctac
     7201 agtacggatc tcttcgaccg cggccgcatc gagcgcctcg tggagcggta cgaggccctg
     7261 ctcgccgcgg ccgtcgccgc ccctgacgcc gcccccgacg gcgacatctg agcgtgccgg
     7321 gcgcgcgtct cgtcacgaga tgtgacaaaa tcggtgtgga cgccacccgg tggtctacta
     7381 ttacgtggac tcggaaaggt atcggcccgc cgcacgcgca aacacaagaa accgcaatga
     7441 acacgaacac gccgctgatg accgaagacg tcgctgaaca ggtgcgcgac agggtggact
     7501 ccgtctaccg gaccgagtcg cgccgcgtgc tggcgaccct gatccgcctg ctcggcgatt
     7561 tcgacctcgc cgaggaggcg ctgcacaacg ccttcgcctc ggcgatggag cagtggccgc
     7621 gggagggtat gcccgccaac ccccgctcgt ggctcgtctc gaccggccgc ttcaaggcga
     7681 tcgacaccat ccgccgccgc gtgcgcttcg atgccacggt ggcggagctg gcgcagcggg
     7741 tcgaggccga ggagtgggac ggcgaggggc tcgaggacga ccggctgcgg ctgatcttca
     7801 cctgctgcca tccggcgctg ccggcggacg cccgggtggc gctgactttg cgcgaggtct
     7861 gcggcctcac caccgaggag atcgcgcgcg ccttcctcgc caccccgccc accatcgccc
     7921 aacggatcgt gcgggccaag aagaagatcc gcgacgcggg catcccctac caggtgccgt
     7981 cgcgggccga cctgccggac cggctggaca cggtgctcga cgtcctctac ctggtgttca
     8041 acgaggggta ctccgcctcg tcgggagact cgctgacgcg gccggacctc tcgggcgagg
     8101 cgatccggct ggggcggctg ctgatggagc tgctgccgga gccggaggtc ctcggcctcc
     8161 tcgccctgat ggtgctgcac gagtcccggc gcgccgcccg cgcctcgccg gacggggaca
     8221 tcgtgctgct cggcgaccag gaccgctcgc tctggaaccg ggagctcatc gccgaggggc
     8281 tggcgctggt ggaacgggcg ctgtcgtcgc gccggttcgg cgtctacacc ctccaggcgg
     8341 cgatctcggc ggtgcacgcc caggcgcccg gcaccgaggc cacggactgg cgccagatcg
     8401 tcgggctcta cgatctgctg gcgcgggcgg ctccgtctcc cgtggtggag ctcaaccgcg
     8461 ccgtggcggt ggcgatgcgc gacggcccgg cggccgggct cgccctcgtc gacgccatcc
     8521 tggagcgcgg cgatctggcc gactatcatc tcgcccactc ggcgcgggcg gacctcctgc
     8581 gccgcctcgg ccgctccgag gagtccgccg ccgcctaccg gaaggcgctc tccctcgccc
     8641 gccaggagcc ggaacgccgg ttcctcgaag ggcggctgcg ggagctgggc gcgggctgag
     8701 ggccggctga agaaaaatcc gcgccccttg tcgattcgag tccgtcccgt tcgactagag
     8761 ggtaggggga ggcccgcggc gagcctcccc ggaaccgaga aaggagccag aatgagcgga
     8821 gtccgggtcc tggtgggtac acgcaagggc gcgttcgtcc tcacggcgga cggcaagcgc
     8881 caaaagtggg atgtcagcgg tccccatttc gcgggctggg agatctacca cgtcaaggga
     8941 tcgcccgtgg acccgaaccg gctgtacgcc tcccagtcga gcggctggtt cggccagctc
     9001 atccagcgct ccgacgacgg cggcaagacc tgggagccgg tggggaacga gttcgtctac
     9061 gacggcgtcc ccggcaccca tcagtggtac gacggcaccc agcacccctg ggagttcaag
     9121 cgcgtctggc acctcgagcc gtcgctcacc gatcccgaca ccgtctatgc cggtgccgag
     9181 gacgccgcca tgttccgcac cacggacggc ggccgcacct ggcacgagct ctccggcctg
     9241 cgccagcacg gctccgggcc ccgctggcag ccgggcgccg gcggcatgtg cctgcacacc
     9301 atcctgctgg acccgcagaa ccccgggcgg atcttcatcg ccatctcgtc cgcgggcgcc
     9361 ttccgcagcg acgacggcgg ccagagctgg aagccgatca accgcggcct caagtcggag
     9421 tacatccccg acccggaggc ggaggtcggc cactgcgtcc accgcatcgc catgcacccg
     9481 tcgcgccccg ccaccctgtt catgcagaag cactgggacg tcatgcgcag cgacgacgcc
     9541 ggcgagagct gggtcgaggt cagcggcaac ctgcccaccg acttcgggtt ccccatcgac
     9601 gtccacgccc acgagccgga gaccatctac gtcgtcccga tcaagagcga ctccgagcac
     9661 ttcccgccgg acggcaagct gcgcgtctac cgcagccgca ccgggggcaa cgagtgggag
     9721 gcgctcacca acggcctgcc gcagagcgac tgctacgtca acgtgctgcg cgacgccatg
     9781 gcggtcgacc aactcgacga gtgcggcatc tacttcggca ccaccggcgg gcaggtgtac
     9841 gcctcggccg acagcggcga cacctgggcc ccgatcgtgc gggacctgcc ggccgtcctg
     9901 tccgtcgagg tccagacgct gccatgatcc gcgtcgtgct gccgttccac ctgcggaacc
     9961 tggcgcgcgt cgacgccgaa gtggaggtcc cggtggacgg cccgccgacg cttcgcgccg
    10021 tcctcgacgc cctcgaaacc cgttacccgg tgctgcgcgg gacgatccgc gagcacggga
    10081 cgcagcgccg ccggccgttc ctgcggttct tcgcctgcga ggaggacatc tcccacgaat
    10141 cgccggatac cccgctgccc gcggaggtcg cgaccgggaa cgagccactg tacgtcgtcg
    10201 gcgccatcgc cggcggatag atacgatccg catttggaga caagcctgtg aaatacatgt
    10261 tgttgacata cggcgacgag aacgctctgg acgagggcga gcggaaccag tgttatcagg
    10321 actccgtcca gctcacccat gagctgcacg cggcggggaa gtacctcggc gcctccccgt
    10381 tgcactccac gtccatggcg acgagcgtcc gggtgcgcga ggggaggaag ctggtgaccg
    10441 acggcccgtt cgccgagacc cgcgagcagt tgggcggcta ctacctgatc gatgccaacg
    10501 atctcgacga agcgatcgcc atcgccgcgc ggatcccgat ggcgctgcgg ggcaccatcg
    10561 agatccgtcc catcgtcgag ctctccggcc ttcccgaaaa gtagtcgccg ctgctgtcga
    10621 ttcgagccat ccccgttcga ctacctgtgg agcccgggcc ggcgcccggg ctcaccaact
    10681 caaccaaaca aggagggggc catgaaaatc gttccttatc tcaacttcga cggtcagtgc
    10741 gaagaggcgt tcaagttcta tgagcagtgc ctcggcggca agatcatggg gattatgaag
    10801 ggcgaggatg cgccgatgcc ggaggagatg gcggcggagt ggaagggccg ggtcatgcac
    10861 gcctgtatgc aggtgggcga ccagctcctg atggcctccg acagcccgcc gggacagcat
    10921 caggcgccgc agggcaccta cgtctcggtc cacatcgacg acccggccga gggccggcgc
    10981 gtcttcgatg tgctggcgga gaacgggaag gtggagatgc cgttcgacaa gaccttctgg
    11041 gccgccggtt tcgggatgtg cgtcgaccgc ttcggcacgc cctggatgat caactgcgcc
    11101 ggcgacaccg ccggccagga ctgtcaggct caggcctagg cagcctctcc ggacgcggtg
    11161 gccccgtcct gggcgccgcg ttccggaagt acatcgattg tcaaagagct ggcggagcag
    11221 ccctgtttcg ggcgccgcca tgaatcgtct cgtccatgtt gaccggaatc gatccgacgt
    11281 gtaaggatag gagctcccgg ggcggctgtc aaccccttcg tgcgcttgcg gccccgtggg
    11341 gcggaggggt tgccccctgg ggcacaggcc ctgccccaag gggccaatgg gccgccccct
    11401 ggggcgaccc tgatcctccg aggggcggcc gacccgcccc aaggggcaca ctggacctgc
    11461 cccggggggc cgccgctcgg ccctagaggg ccggcacggc accaaccggg gcgagccggt
    11521 cgccctccgg ggccaaccgt cgacgtcgcg gggcgaacag cccacgaagt cgccgggctc
    11581 tttgaaaact gaatcgtgac ggggccgcgg ccgagtccaa ggccgcgccc ctgaggattt
    11641 ctccgtccaa caggaggacc gtggggggag gcgaaggagg agggcaccgg tacgatggcg
    11701 gaccgctctg gccgacaagg acaacgggcc gccaatccat tgaccgtcta gtcctttttc
    11761 tgctcctagt tgaaggagag caaagcccgc cgtgcgccac acccggtccg gtcacccgga
    11821 ccccatacca cacggttgag gccgacatag atggcccgct ggtccccctc gggaaccgtg
    11881 gtatccggaa tggcgaagag attctgggtc gcgggggtct gcacatagct gtgcggcggt
    11941 ccccaactca cgaactgatc cttcactacg actttcaggg tggcgtccgt gagattcatg
    12001 aactgtcgga tcaaggggtt gtcgataccc aagggcagga gcatgttggc aggcgccagc
    12061 gcaagaatct cgtgcgccac ccaaagctct gcctcgtgct cggtggtccc aaagtcaccc
    12121 gagtgtccgt cgttggtctt ggagtccccg atgtgcaccg accaccctgc cggacggccc
    12181 tcatactcga ccacgatatt ggcttgcgtg caggttggtg ggatgaccac cttgagggcg
    12241 gcctgcttcc aacccgtcgc cggacagaac tcagtcgccg gggctggtga acagttcccg
    12301 gcgacgttgc cgggctctac gagcgtgtag gtgatgtagg gggtgacact ggtatgcgac
    12361 gccttctggc aggctttgag atcgatgatg aactgaccag ccgggcactg cgcctggagg
    12421 gcaagcggga atgcggcaag gacggcaaag acgaagatgc aaacgaagaa atgaatcggg
    12481 tgcctacgga ccattttgat ctccttttgt gagtgattca agttgtcgat ccgccaaccg
    12541 tgctctctgg ctcttcatca gtatagaact catcaagctt taaaccaaga ctaaaataat
    12601 ttttgccgcg tccaagcttt cccggctccg agaggagctc acgcaacgat aggaggcggc
    12661 aggagagctc agagcgttcc tactctcaag cggtaggtcc gcagtacagt agatgtgaca
    12721 gttgcgttgg aggcatagcc atatacgcaa gcagaatagg ttcgaataga tttccaagaa
    12781 taggttccag gtgtttcgca gggagtgtcg gctcgtagac ggccgccgca gccgccgcaa
    12841 gaataggtcc aggtgtttcg cagggagtgc cggctcgtag acggccagca caggcagagc
    12901 atcgtactga aaggcgtact cgccctcgct tttcaggcct tcggtcgcat tgaacgggtg
    12961 ggcacggcgt gagcttgccg acgggcagac ggaccggcac acctccggca ggtgccagat
    13021 cctctttggg cgattggagg acggctaggc ccgagggcct atctatctgg tgggtgagag
    13081 ggctggagaa tcgtctcgca cgaaggggag gtggggtgga aagctaccgg taggaaatgc
    13141 cgccatgcgg tctcctgcgc gtagcttctc ggctgcctcc ctgaacgcag cgacgaagct
    13201 tgcataggcc tcgtacaacc cgcggcgtac tttcttggtg aacgcgtgga agcgcggcgc
    13261 ggaggacttc tttgggttcc ttgggcgctc gaacggattc tgccgctcga tggcttcacg
    13321 gcccagcggc tcgatcccgc tggcttcgcg gagcgccgcc gcctcggctt cgatctgctg
    13381 gacgagtccg gcaatcctgg agcggcggat ctcggcggaa aggccatccc agcagggaag
    13441 ggggacgagc tccaggatct gcgtctcggc aaagctccgg gtttcgcagg cctcccgcct
    13501 gcgccgggcg ccgtactcct gggtgcggtc aaaccacgtc cccgcgacag ggcttccgtc
    13561 aaggagagcc tgcgcggcgt ggactcccgg ccaatccgcc agccgttcga ccaacccctc
    13621 cttgcagcca ttcgagaggg tatagacgag acggtcgatc tgggcggctt cctcgccgct
    13681 gacgacgatg gagtggtagc ggccctccca gaagtgcccc ctgcgccccc ggagtcggtt
    13741 gatctcgcgg gcgacgttgg agttgaggta tccggcgaag cgggcgagtt gctcggcatc
    13801 gtggacccgg accagaaggt ggtaatgcga ggacatgaag cagtaggcca cgacgccgac
    13861 ctggtgctcg ttggccgccc gggcgagcac gccgcggatg atctcgttga ccaaggggct
    13921 gggagtcagc aggtactggc tctggaagca ccgggtggtg atctcgacga gagtgtcgtt
    13981 ctccgggacg aagcgcaggg ggcgtggcat ggcggagccc cgtcagggca agccccctgc
    14041 cagctcgaga aattcgccta agtcactgat ttggcaggga cgtgtagctg acaggcggca
    14101 agggttcgcc atccaccatg tcaaggaatc tggcgccttg aggtggggag agtctggtct
    14161 gccctcttgc ctccggcttc ggagtgtgtc aaaagatctg gacagcccgc tcgatcttct
    14221 gacagtggat cgggcggaca agacgactga ctagagggcg actgcctaga ctagagggct
    14281 ggcacttgtt ctgttgagcc atcacgcctg ttcttctctg gaaatgactg tgagctcgtc
    14341 agcaaatccc atttcactgg atcgggtttt tcccatcctg gcggtcgccg acctcgccgc
    14401 ggccgtggtg tactacactg atgtccttgg gtttcatgaa gcatggcgct ggggcgaccc
    14461 gcccgctcgt gtcggagtcg cccgggatag ggtagagcta caactggtgc aggcgggaac
    14521 gccgggcgcc cccgttgggg acgccgtggt ctattgccat gtgcgtggcg tggatgcgct
    14581 gtacgccgag tgcaccactc gcggcgccga catctgtatg ccgctggcgg accgggggtt
    14641 cggacttcga gattttcgag tccgggacct ggacggaaat atcctcggct tcggggagcc
    14701 gatcaccttg acccgcagta gctcaaagtc agaggacacc atgacgaggc gcgacggaga
    14761 gaacctgagc gagatgctca accaacacgg cggcgcattg cgagtcgcca cggccgccga
    14821 catcccggcg attttcgcgg tccggaccag tgtgcgcgag aaccatctcg acctcgatgg
    14881 gctcgccgag cggggcgtca cgccggcgtc cgtcgcggga atgctcgacg aggcgcaggc
    14941 gcgcacctgg gtggtcgagg aacgcgggga ggtcgtggca ttcagcgtgg cgaacgcgca
    15001 ggcgggcacc gtattcgccc ttttcgtcca cccgaaggcg gaggggcgcg gctatggccc
    15061 ggcgctgctt cgcgccgccg aggagtggct gttcgcggca ggctgggagg tcatctggct
    15121 gaatacgggg caggagccgc acctccgggc gcaccgcgtc taccgaactg ccggatggga
    15181 gctcgtcggg ccagccgacc acggtgacgt gcggtatgag aagcgccgtg ctacctgacg
    15241 agacggggac aggtgagccg tccctgatca tcgactcatg ataggatccc ttcgacacaa
    15301 gaaaggagct cccatgaagc gagccatcgc agccctgttc ggaatcctgt gcgctacggc
    15361 gtccttcgcc gggacggcgg tcgttaccgc cagctcggac agcggagggt acgagctcac
    15421 cgtcaccact acaccaaacg ccgaaaaggc ggatgtcttc gtgggctcgg tggtgctgaa
    15481 gaataaggcg accggcgaag tcctcgccgc gccgaaggtg atcttcaagg caggagaaac
    15541 ggcgaacctt caaattcagg gcgagggtcg cacgcttcgg ctcgagctcc attccgatgc
    15601 gacgaaaaac gaagcgaagg cggtcgtgga atactcgctc gcggacgagg tggtgttcgc
    15661 gccgactgtc atcctcgagt tgaggtagtc cgcggcctgg cagcagctcc ctgccaggcc
    15721 gaagatcccc ccttcgccta ctgctccctc aacgcctcgg tcgactcgac ctggagcccc
    15781 tctgggcaga ctcgaccgag atcgagatgg ttctcgcgca cgctgcgacg ggctcgccga
    15841 gcggggcgtc acgccggcgt ccgtcgcggg aatgctcgac gagccgcagg cgcgcacctg
    15901 ggtggtcgag gaagacgggg aggtcgtggc attcagcatg gcggactcgc gggcgggcac
    15961 cgtgttcgcc cttttcgtcc acccgaaggc gcacgggcgc ggctacggcc gtgcgctgct
    16021 ccgcgcggcc gaggagtggc tgttcgcggc aggctgggag gtcatctggc tgaatacggg
    16081 gcaggagccg cacttccggg cacaccggat ctaccgggct gccgggtggg agctcgtcgg
    16141 gccggccgac cacggcgaca tgcggtatga gaagcgccgt gccgtctgac ctggcccgac
    16201 ctgaacattc cgttcgctga acaggcaaga atggctaagc gtcaacttag ttgacgctgt
    16261 cggccaggcg gtctagactc cccaggagat ttcagatgag aaggtttgcg ctcgggccgt
    16321 gctctgacca gccggcccac ccggcttcac tggagaaggc gttccacgtc tatagagttg
    16381 cactgggcga ggggctcctg caagccaagc ctattgaggg ggagaatact atgagagcga
    16441 agcttcgagt agggtgtatc ggccttctgc tctgcgtgat cttgacggcc aacgcggcgg
    16501 cggaggactt tttgatgtgg cagatgccag gcacgggcat ctcgtttccc gtctatgtgt
    16561 atagcggcgg aagccaggta ggctatatcc cggatagtac tttgcaggtc ttcgtggggc
    16621 cctatgccgc ggggaccact tctggcagct accaactgta ttatcaagcc ggcgggtcct
    16681 ggtacgcatg tgctctggtt ttggcgagcg gaagcatcga tgccagtacg acttgcccgg
    16741 gagcagtcat caacccgccg gtatcccaga atggcgccca atccaacgtc tatacagtgg
    16801 gtgtggctgc aagcgcctgg ccggcggccg tggccgcgcc atccaacccg gtcgagacgg
    16861 actacggtca acgcacgatc accttggtca acaacaccaa gtatccgatg atccagatcg
    16921 gacagtcggc cacccagaac acaccgatcg ctgccacgat caagaagaat gcgcccttcg
    16981 tggtgagcgt cgggaccaat ggcctgagct cggcggcatt ctatctgtcc agctattgca
    17041 cggctgggtc ggtggctgaa tgtggcgagc cgccgacggt cgcgcaatgc tctggtgagc
    17101 cgcccaagaa ttgggtctgt acgggaggct attttccggg ccaggttccc tatgccacca
    17161 agatcgaagc gacgatattg gcggtcagcg acggcgttcc cgcgggcgcg agcaacgtcg
    17221 atgtctctgc ggtggacggc tataacaccg gagtgaggct ttatcctgcc gcgccaacgt
    17281 actgcacatt cactgtgccg cctgaaaact cgaatgtgct tggagcgggg tactacagct
    17341 ccgcgaacgc cctggctcag gtcgtacccg taagctcgag ctctttgtcc gccatgtgcg
    17401 agaagagctc gcagctgccc gggcctccct cgaaggggca gacggcctgg aatctgtcca
    17461 aggtctcgtc gagcaacgcg ttcgaaggtt gtgtaagccc ctgcacttat gcgaccgcca
    17521 acgtgaacca gaatggcgtc accgagcagg atgtcaacct gttctgctgt agtgggccat
    17581 acaacacgcc tcaaacctgc ggtatgcctg ccggccaact gggcgccaac acctccacgt
    17641 acaacaccag catcgagagc tcctcgctat tcaagaacgt atacggctac gccttcggcg
    17701 acgcgggcag cgactatgct tgcccgccgg agaccaactt catcgtagag tttgtttcca
    17761 cgccctgatt cagccccgaa aagtaggcct gcgcgcggac gaggccgcgc acagccggcc
    17821 gaccacggcg acgtgcggta tgagaagcgc cgtgctccgc tacgaagctc ccccttcgcc
    17881 tctggccaca aacagattcc gaagtcttga cgagcatgcg aaatgccgct aagctggcgc
    17941 agtatcaaag actctgcagt gaaggggctg aaaggctcct ttgtattcgt gcgcagctcg
    18001 ggcgaacgca ggtattggag cgtttctccg ggctgaagca cgaatctgga acatctatct
    18061 ctttggagag cgctcatgac tacccaaagg agcggcgtga gccgcttcgc acagatttcg
    18121 tccgtggctc gatttcgcga ttgcctcctg atgatcttgc tgttctctgc tggttgtgcg
    18181 acaacgtcct ctccaaatct tgggacagtc gcttcccacc ccggccacaa cgatgcattg
    18241 gagaggagtg accacactcg gcatgttccc gctgtgagct gcaaattgcg gcaccatgat
    18301 gacgaatgcc cgcccccgaa ggaaggaggt gcctgggaga gcgtgatccc tgcaacggga
    18361 agtgccgcca tccacaatat tctcggcatg cagacggtgc actctacagt cctgccgagc
    18421 gggcgtttgc tgctggtgag cggtagcagt tggcgcaacc ggaatgggac ccagtattac
    18481 ccggagtatc ccgagcccga gacgcctacg gggttgttta tcagggatga ggatcccttt
    18541 cgcaacgaga agctcgatga atactacgag ctcgtgaaca acgcagccat ctacgacccg
    18601 gtcgccaata ccttctatcg ggtccctgta ccggtacccg aagccgatcc tgacaacgcc
    18661 gggcacttcg ctcccaatga cttcttctgt acggggcacc agcatctccg ggatggcaac
    18721 gtgctcttca ccggaggaac gcagtactac agcccatttc ggaccggcaa caacacctcg
    18781 tatatcttcg attggcggtc ggagttggag acctcctggg ataaggtgga ctggcgggtg
    18841 cggccggcct ccaatgacga gacgccctgg acgttctccg gtttcatgaa acgaggccgt
    18901 tggtatccga gcctgcttcc tctgctcgat ggccggttgg ctgtcttcag cggcttcgtg
    18961 ggtttcgaca aagggttccc cgagatgcac atcttcgaga tcaacagttg gatcgaggtt
    19021 ttcgatccat cggctttcga ccccgccact ccagagcggg cttggaaggc ggttgacgtc
    19081 gcgcaggtcg agaacagccc gttcagaacc ctcatcaacc ccgactttgt accgactcct
    19141 gacgtgcctt gcgatgcgcg gtgcatcgag tccaacaaat acgatgcctt caagctctac
    19201 cccgagaact accttcaacc cgatgggcgc atctatttga ctcgcgaggg ggactgggtc
    19261 agcctgcgca cgtgcgacgc cgccttcatg cggcgcacga agcacaccta ttgggccacc
    19321 ctggaggacg agaacgacgg gctccaggta tcgttttccc gtgggcccga ccgggtcgag
    19381 gacgtgactt cctacggtac gacgttcaag gatcccgaaa ctggccagat tcacctgatc
    19441 gggggccaac cgacatcgcc gggaaccctg tatccactca actcggccaa ccccaaccac
    19501 tttgcgggcg gccggggttc ggcacggctc gagaccttca ccccggacaa cagcccggcg
    19561 ggaggccatt ggaccctcga tccggacttc ctcgggactg agccggaggc cgaccggacc
    19621 atgcactata ccgtcgccct gccgacaggc cagattctca tcatcagcgg cggcaattac
    19681 gacttctatg ggccggtgtt tcatcccatc ctgctgacgc ctcagtacga tccgaagacg
    19741 ggaaagcgga caggctattc acaaaagaag atgctcgatg ctctcgagcc ccggctgtac
    19801 cacaatatcg ctctgctgct gcccgacggg cgagtcttcg tctcgggagg caatagcgcg
    19861 cgagccacta tcactccgcg caatggtccg ccgccgccgt attccggggc cggccagccg
    19921 cctccagacc tcaagcaggt cgagaccgac ctctacttct tcgatgatgg tccgataggg
    19981 aagggtttac ccgggcaact caccacgccg acggagaact ggacggcgga gatctattca
    20041 ccgccgtatc tgttcatcga tccggagcgc cgagcggcga tcacatctct cgcgccgatc
    20101 gagaaggtcg gctatacgcc gtcggcctcg atcggcggca agaacttcac actctttcat
    20161 agtggggttg cctaccagat ggggctcaag gccctgccca actgcgcacc ggaggatgcc
    20221 cgcctggcgc tcatcaagct tcccagcgct acccatggct ggcagaacgg ccagcagttc
    20281 attcaactcg acttcgagcc ggccgaaggc ggcatcaagt tcacgacgcc ggacgcgaaa
    20341 gcgagcatga taccgccggc ctactacatg ctgttctaca cggattgcca cggcaagcct
    20401 gccgaggcat ggatggtgcg cttcgacgac gcggcttcgg agccgtaacc ggtaacgcgg
    20461 agccggaggc tgggcccgcg tgcggatcaa gccgcacgcg ggcccgggag atcacctcga
    20521 tgtgatcaga tgcaggcgag gttcgagcag gtgcgccatc cggtcggccc cagcacgaag
    20581 tgataggtcg gattggccgc cggcagggtg atccagtggt tcgatccaca gcccggggtc
    20641 gagaggtcgt ccacgtagac cctgcgcgtg ccggaatcga ggcaggactc ggtgctctgc
    20701 gcgagcacga acaggagatc gagctgccgg gtggcggggt cgtacatcgc ggtatagacg
    20761 gtacccttcc cgccgttgac cgcgatgttg ttgttgagct gaggcccggt gtagaacgtg
    20821 gcgcggcggc cgtcgctcca cagcaggaag tggaagtcgg ctctcatccg ggccgcgccg
    20881 aacaccgtgg agagagagcc cgggatggtg gtgttgttca ccagagcctg gagctggctc
    20941 ttctcggtcg acgtgaggcc gaagacggcg gagtattgcg ccgcgagata attcaccacg
    21001 tccctcacct gcggcgactt gtagcaatcg gtcgagaaac ccgcggcggt gccgaacagc
    21061 cagcgcttcg agtcctggtt gtagtcggtc ttcgagcggg ggctcgccat ggcgagcacg
    21121 gggcagttcc ggttgttggt ctggacgagg ctggttgtgg caccctgcac gttgtagacg
    21181 ctcgtaaagt cgacggccga cgcggagagc gcgtaggcgt accaggtgcg gcgcagataa
    21241 tcctgcgcct tggtcttgat cgcggccgcc gccggatccg cgggatgcga ctgcgcccag
    21301 tagtgcgccg ccaagactcc ggtcatcgtg gccgcgtcgt agacgttcga gaacagctca
    21361 gagcccttga agtgccagat gtttcccgcg ccgctccccg aggcctggcg atcgaggaag
    21421 tcctgccacc aagtccggta atcggtgcca ttgacgatcg ggccgtcgaa cagcgcgacc
    21481 gccgcggcga tcacgggctt gttctcgctg ccggcgttcg cccactggtt gaaggtcggg
    21541 cccaactgcg ccgaatagtt gacgttggct gaaggaacgc cggtcttcat gacgcagagg
    21601 agctcgttca ggagatcggg cggatctccc gggtcttcac cggtaggccc gcattccaga
    21661 ggcacggcgc catcggtctg gaggatgagt tgcggctgat aagcgccgta ctcctcctcg
    21721 ctgtagaagc gctggcccgt gatatcggcc gagctggcga gggcgaaggt gaccggggag
    21781 ggagtgatcc agttggtgac gtcgaggtcc aggaaggtgt tggccgccag gttggtgcgc
    21841 gatccgatga aggagaagac cgttccgctc gaccagttgt tccaggtgag cgaggtctcg
    21901 gaccaggagg gagagcccat gctgacgtgg tagtaaccca cctcgctgat cgctcccgcg
    21961 acccgcaggc gcagcgtcgc ggataggatg ttcccgtcga gggccgggat attgaaacgg
    22021 aggaaggagt gttgcccctg cccggtcgcg acattgcgga tccgcaggtt gagatcggag
    22081 ccgtagttgg agttgggcgt cgactgctgg acgaacgtgt ccgcggcggg agtcaggctg
    22141 taggtggttg cggagagggt tcctggcagg gagaccagga gggttgtcgg gaggagcatc
    22201 gccaatgtca actttttcgg tttcatcgtc agcatctcca tctagctcgg ctcgggctga
    22261 gcgctcagcc cgagccgata aaatggatat ttgcatggaa cgatgacgcg gctctaacaa
    22321 aatcttgctc ggttggtggt ggcgctttct agtgccggtc gtctggccgg ttgcccggaa
    22381 accggagctc cccctcgcgg atctcgtgct tccacagctc cttgccatgg gtgtcgaggg
    22441 tgcggagggt cagcacccgc tccttgggct tgccggagac ctcgacgagg ccgaagttgt
    22501 ggacgccgcc ggtcacccag gtgccgggga cgcgggcggg gttgtctgcc tccttctcca
    22561 ggcgcgagcc gccggaggtc agggggctcg aggtgaactc gtagagcggg tagaggccgg
    22621 gcagttggcg gcggagcagc tcggtgtggt ggcggtcccc cgacaggaag accaccccct
    22681 ccacgcggga gtcgcgcacg aagtcgaaga gctccttctg ctcggcgggg aagttgccga
    22741 acgcctcgaa gaaggtgagc gggttgacca tctggctgcc cgacaccacc accttgaagg
    22801 tcgcgtggct gccggccagg gcctccttga gccagtccaa ctgctcctta cccagcatcc
    22861 gcttgcccgg gccgtccggc atgtcgtcgg gcgagcgata gtagcggtcg tcgaggagga
    22921 agaactcgac gtccgcccac tcgaagcggg tgaagacgcc gggcgcctcc gccgtgccga
    22981 aggaggggtt ggggaagtgg tcacggaaga cgcgcaggct ctcctcgcgc agccggtaag
    23041 tgcggtccgc gttgttcggg ccgtagtcgt ggtcgtccca gatcgcgtag tggtgcaccg
    23101 tgccgagcag cgcctgcatc tgggggagcg cccggtcgtg cgcccagcgg tagcgcatgc
    23161 cggcctcggt cagccagtcc ggcccccggt aatagacatt gtcccccagc cagatcatga
    23221 agtccggctt ggccgccgcg atcgactcga agatctcgta gctctttcca tagggctcgc
    23281 cgggacggtc gaacggcggg tcgttgatgt aggcgcagga gccgatggcc gcccggaagc
    23341 tcggcgggtc ggtgcggtat tgccagtagg cctgggtctg gagcgtcagc gggtagggcc
    23401 gctccacccg cttgccgtcg aggtagacct cgtagtcgta gcgggtgccg aagtcgagcc
    23461 cggagaggcg cagcagggcg atgcccatgt cgccggtggt ggtcacctcc tgggagaggc
    23521 gggcggcctc ggggcgtccc tgcggccaaa ggcggacctg caccttgcag gggtagcggg
    23581 tctggaccca gaccgtcgcc tcgtcgacct cggccgggcc gagcatcggg ccggagcgca
    23641 ggcagtggcc gggatcgttc gccgcgggct ggccgcaggc ggggagggcg agggcgcaga
    23701 gagccagggc gagcgccagg gatcgcgatg agattcggga cacgagcggt tcctccttgg
    23761 ggatctcttt cgagggaggg ggagtctagc aggctcccat ggcgccggtg cgatagactt
    23821 gggccggtca acctacggga gggagctcat gagcgacagc gtctggtacg tagcggtcca
    23881 agggcagcag cagggcccgc tgagtcagga tgaagtgatc aacggcatcc ggagcggccg
    23941 tttcggccgc gacgcccacg tcttcagcca ggggatggac aactgggccc ccatctccag
    24001 ccgcgccgag ttcgcgcagg cgttcgccgg cgcggcgtcg atggcaccgc cgcccccgcc
    24061 gccgcgcggc gccggagcgc acgacatcga gtacgagctg ttcggcgagg agatgcagta
    24121 cgtcgagatc actctcgacc ccggcgaggc ctgcatcgcc gaggccggcg ccttcatgtt
    24181 catggacccc ggcatccaga tgcagaccat cttcggcgac ggctccggcc aggagggggg
    24241 cggcttcatg ggcaagctcc tgtccgccgg caagcggatg gtcaccggcg agtcgctgtt
    24301 catgaccatg ttcggcaaca gcggcggcaa caagcaaaag gtggccttcg cctcccccta
    24361 cccgggcaag atcatcccca tcgacctgcg ccgccacggc ggccgggtgc tgtgcgagaa
    24421 ggacgccttc ctgtgcgcgg ccaaggggat ctcggtgggg atcgccttcc agcgccggct
    24481 gggcgccggc ctgttcggcg gcgagggctt catcctgcag aagctggagg gagacggcct
    24541 ggcgttcgtc cactcgggcg gcaccatcct ctcgcgcgag ctcgcccccg gcgagacgct
    24601 gcgggtggac accggctgcc tggtcgcctt cgagagccag gtgacctacg acatccagat
    24661 ggtgagcggc atcaagaccg ccttcttcgg cggcgagggg ctgttctacg ccgcgctcac
    24721 cggacccggt cggatctggc tgcagtcgct gccgttcagc cgcctggcga agaagatctt
    24781 cggcgccatc ccgcagcaga agggggaggg ctccatcctc ggcggagtgg gcctgggcgc
    24841 ccttctggga ggcgacagct aggagccttc agctcaccgg ggcctcgatc ttgagcatcc
    24901 ggccccaggc ctggcggagg ttctcgggga ggtccaggga ggggaagacc tcccggatct
    24961 cctcgcggct gacgaaggcc cagatgtcgt cccactgggc gtagcgcagc aggtgcgaga
    25021 tcacccagga gcgctcctcg tcggtgccct ggcggaggat gcggcgcacc tcctgggagc
    25081 tcacccgccg gtcgggaaag aagtcgaggg gatcggtgga ctgccggccg cgcactggcc
    25141 cgttcatcca ctgagtatag cccccttgac cgcgccccac ggctccgtta catttccctc
    25201 gctcgattgt cgggcgggca taactcagtt ggtagagtgc aagcttccca agcttgatgt
    25261 cgcgggttcg agtcccgttg cccgctccag attctttcct tcccgggcgg acggtgcgtt
    25321 ggcaccggcc gccctcttga tgtcaggaga ccagggaccg cccaccgccg acggccggcc
    25381 ggtgcgcgga gctgaacgcc atgtcgatct ttcgtcgtga tcgtgagagc acgggctcgg
    25441 ccccgtcctc tccctccccg gccagcgagg ccccggcggc gtcctcccag cggcgccgca
    25501 tcacccacat cgcgcccggc acccgcatcc ggggcgaggt cacaggctcc agcgagctcc
    25561 tcgtcgaggg ggaggtcgag ggggacatcc gggtcgactc ggtggtggtg gtcggtgccg
    25621 aggggagcat ccagggcccc atcaacgccc aggtggtgcg gatcggcggc cgggtgaccg
    25681 gcaacgtctc cgccgccgag cgggtggagg tctccccggc cggcagcgtc gagggggaca
    25741 tcgccgcgtc gcgggtggtg atcgctgaag gtgcgttttt caagggcagg atcgagatgt
    25801 caggtgagaa gaacgcggca ggccggagcg gcaagggccc gggcgagccg tccaaagctg
    25861 cgggcgagcc gcagaaagct ccggccacgc cgggaaaggc ctagcatgat cgtcggcatc
    25921 cccaaggaga tcaagaatca cgagtaccgc gtcggcatga ccccggcgtc cgtgcacgcc
    25981 ctcatcgagg cggggcagac ggtgctggtg gagaagagcg ccgggctggg cagcggcatc
    26041 tcggacgacg tctacgtcga ggccggggcc gagatcgtgc ccgatgccgc cgccgtctac
    26101 ggccggtcgg acatgatcat caaggtcaag gagcccatcg agccggagta cgagctgctg
    26161 cggcccggcc agctcctctt cacctatctc catctcgcac cgctgccgcg gctcaccgac
    26221 gtgctgctcg atcgccaggt caccggcgtc gcctacgaga ccatcgagga ccgccagggg
    26281 cggctgccgc tgctcacccc gatgtcggag gtggcgggcc gcatggcggt ggtggtgggg
    26341 gcgagctacc tgctgcggag ctacggcggc cgcggcacgc tgctggccgg agtgccgggc
    26401 gtcccgccgg gagacgtggt ggtcatcggc ggcggcatcg tcggcctcaa ctccatcaag
    26461 atggcgctcg ggctcggcgc cagcgtcacg gtgctcgaca ccaacctcga ccgcctgcgc
    26521 tacatcgacg acctgttcca cggcgcggtg accaccctcg ccagcaaccg ctacaacctc
    26581 ctggcggcgc tgcggcgcac cgacctgctg atcggcgcgg tgctgatccc cggcgcctcc
    26641 gcccccaagc tggtgacccg cgacatgctc aagcagatga aggagggggc ggtgatcgtc
    26701 gacgtggcgg tggaccaggg gggctgcttc gagaccaccc ggcccaccac ccactccgat
    26761 ccggtctacg tggtcgacgg cgtcgtccac tactgtgtag ccaacatgcc cggcgcggtg
    26821 ccccgcacct cgaccttcgc gctcaacaac gccaccctgc cgtacgccct ggcgctggtg
    26881 agcaagggct tcgagcgcgc ggtgaaggag gacccgggcc tcaggctggg ggtcaacacc
    26941 taccgcggcc acatcacctc gaagccggtg gccgagtccc aggaccggcc gtaccggccg
    27001 cttgacgagc tgttgtagga ggaggcgctc atgctcacgc ggcggcgttt cctcgacctc
    27061 accggccgcg ccgtcgcggg tctcggcgcc gcccacgtcc tcggcccgat gcccctgatg
    27121 gcggcgcaag ccgccgccgt gcccaagagc gtggcggtgc cgccggccgc gctggaggtc
    27181 atcgacgctt tcgtgaagcg tcacatggag gagtggggag cccccgggct gaccctgggg
    27241 gtcgccggcc gtgacggcgt gatcgccgcc cgcggctacg gcctgcgcga tcgggagagc
    27301 cggacgccgg tctcccccga ggatctcttc cagatcggct cgatcaccaa gtcattcatc
    27361 ggcctctgcg cgctgcagct ccaccaggag ggcaagctcg acctcgagcg gccggtgcgc
    27421 gagtacctcc cctggctgcg catcgagagc tcgtacgcgc cgattacccc ccatcacctg
    27481 ctgacccact cgtcggggct gcccaacccg ctcgccctgc cgcgcgagcc gctctggacc
    27541 gggcacgcgc cgggcgccca cttccactac tgcaacctgg gctacaacat cctcggcctg
    27601 ctgctcacgc agttggacgg gcggcccttg cgggacatgg tgcgggcgcg gatcttcgag
    27661 ccgctgggca tgagcgccag cgagccggtg gtctcacagg cgatccggcc gcgcctcgcg
    27721 gcgagctaca tgccgctcta cgacgaccgc cccttcccgc gccgcggcga gctcaccgag
    27781 gcgccgtacc tggagatgga caacgccgcc ggctgcatcg cgtcgacacc ccgggacatg
    27841 gcggtctaca tgcagatgct gctcaaccgt ggccggggct ccaagggggt ggtggtctcg
    27901 ccggagagct tcgcgcgctt cgccaccgcg gcggtcccgg cgccggcctt cgggccgacc
    27961 gcgggctacg gctacggcat cgccgtggcg cccaccgacg ggcggctgat cctgcgccat
    28021 accggcggca tggtctcttt cagctcggcc atgcatctcg acatggacgc cggccttggt
    28081 gtcttcgcct cggtcaacgc ccggctcggc ggctaccggc cgaacgcggt gtgcgcctac
    28141 gcgctctccg tgctgcgcgc cgccctggcg ggggaggcgc tcccgtcgcc gcccgagccc
    28201 gacgatccgt tccgggtgcc caacgccgcc gactacgccg gcacctacac catgccggac
    28261 ggccgcgccc tggtggtcgc cgccgcgggg gagcgtctgg aggtcgtgta tggcggacgg
    28321 cgggccgcct tggagcgggc ggcgccggac gccttcctca cgcagcatcc ggacttcatc
    28381 ctggatcccc tggtcttcga gcgcgagagc gacgccgtga cgcaggtgat ccacggcggc
    28441 gactggtacg ccgggacgcg ctacaccggc cccaagacct ttgacgcgcc cgccgagtgg
    28501 aaggcctacg ccgggcaata ccgcaacgag aacccgtggg aggggagcgc gcgggtggac
    28561 ctgcgccagg gccgcctttg gctcggcggc gccgagctgg tgcctctcga gcccggcctt
    28621 ttccgcatcg gtgaggagga tcacggtccc gaccgcgtgc gcttcgagac ggtggcgaac
    28681 ggccgggcgc tccacgccgt cttctcgggg acggagtacc tgcgggtgga gacgtagggc
    28741 acctctcaca cctcaggcca cggcgttccc gtggcctcca tccacagctc ccgcagatcc
    28801 gccggcaccg gcgcctcgaa ggccaggcgc ctgccgtccg ccgggtgatc gaagctcagc
    28861 cgccaggcgt ggagggcggg ccgggtgaag tcccgcagcg gcgcctggac cggacggggg
    28921 agccccttcc agcgcgcctc gccgtagacc gggtcgccga tcagcgggtg gccggcggtc
    28981 ttcatgtgca cccggatctg atgggtgcgc cccgtgccca ggccgatctc caccagcgcg
    29041 atgccggcgc gcgccgccac cgtccggtag tcggtgcgcg ccggccggcc gccgggccgc
    29101 accgccatct ccttgcgccg gtcggggtga cggccgatcg gtgactcaat ggtgccggcc
    29161 ggtggagagg gcgtcccgta gacgagggcc aggtagcgct tctcgacctg ccgctcggcg
    29221 aaggcgcggg agagacgggc gtaggcgccg gccgtccgcg ccacggccat gacgccgctc
    29281 gtcccctggt ccagacggtg gacgatgccg gggcggcccg ggccgccgac gccggccatc
    29341 tcggggtagc ggtcgaggag acggtgggcg agggtgccgg tggcccggcc ggcgccgggg
    29401 tgaacggtca ggccgggcgg cttgtcgagc accacgacgt gctcgtcttc atggaggacg
    29461 gggagctcgc cggactcggg gacgatgcgg tcgtcccgga gcttgggacg gtcgcaggtc
    29521 acctgatcgc cggcggcgac ggcggtcgag gggcgggcct cccggccgtt gacctggacg
    29581 aggccgtcgc ggatccagcg ctggacctgg ttgcggggga ggccgaggcg ttcggcgacg
    29641 tgccggtcca ggcgctcgcc cgcgg
//
LOCUS       DASPJI010000683         4493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_390605, whole genome shotgun sequence.
ACCESSION   DASPJI010000683 DASPJI010000000
VERSION     DASPJI010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390605"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VEL74_19370"
     CDS             <1..252
                     /locus_tag="VEL74_19370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64749.1"
                     /translation="WRRRLESLGWASLATVFFDLFLILFQVKAVYATQLGDWSANHYG
                     PVARNLYGLGRHLLDLPFKLGLPLLLWAGFYLDELPGRS"
     gene            complement(329..556)
                     /locus_tag="VEL74_19375"
     CDS             complement(329..556)
                     /locus_tag="VEL74_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007950694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64750.1"
                     /translation="MARQNYQHGKRQRDLAKKQKREEKLAQKKERKKNPLGAPIENPD
                     QPVDPDQPVGLLEDEDDDDGDDESEDETQDS"
     gene            760..3072
                     /locus_tag="VEL74_19380"
     CDS             760..3072
                     /locus_tag="VEL74_19380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64751.1"
                     /translation="MTGHRQHRRHPGGRALTAALLGLLLLPGALAPAPAPPDLEPPRA
                     LTPRGLANLMAFSRLLGYVRYFHPSDQAAAVDWNTLAIAGVQAAEPAADAVALARTLE
                     DFFHPIAPALRVFAGGRRPELPAEILPPADTDPTKTAWHHLGGGTGGPFHSHRFQRSI
                     GEPGRLRQSLVAAPLLGKKVRFKAAVRAEVEPPHEARLSLLVDLPGAEEPREVTIPSG
                     GWQTLEIVADIPAEARDVEAGVLLLHQGRVWIDGAVLEVVGEEGGNLLRNPGFERGNT
                     GTLPFGWSVPRGADEAGYVAEIREERPAEGRRCAALSWVEPEPVFPKPHEPFLANLGG
                     GVAALVPMALYVDGQGTRPPVPPEVTPPAPAKPARFEPSGQDRATRLAAVALAWSALE
                     HFYPFFEETGTDWNGALRRALTSAATDPGDAAFIRTLQRLVAALQDGQARVAGPAIPQ
                     RFLLPILWDWIEGRLVVTAVLPKKAGEIVPGDVILSLDGRPAEEALAAAEELTAAATS
                     GARRFAAVRTLLAGNPFEAVQVEVQPFRGEPFSTTLIRSISPEGDKDLQEPRPEPVAG
                     LDRGVLYLDLIRMDPADVRRAMALIPEATGIVFDARAPMPGPGMDNNIRRFLPLLTVD
                     SLEMPRRSVAVITRPDRRAWQDYPVSHLSPQMPRFEGEVAFLMNSGVIGELESFLTLV
                     EHYRIGALVGSPTAATIGEVSSIRLPGGYAVYWTGTRIRKQDGSRHHAVGIQPQLDVV
                     RSRGLIAAGRDEVLEKAWALVAPPVERQEE"
     gene            complement(3180..4031)
                     /locus_tag="VEL74_19385"
     CDS             complement(3180..4031)
                     /locus_tag="VEL74_19385"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HYG64752.1"
                     /translation="MKAYFALALVLSLLLLTPAVQAEAPRIPWVSPASKVGLTIGITD
                     VEIVYNRPAVKGRTIWGGLVPYGEVWRMGANAATTLSFSTPVQVAGQEVPAGTYALFA
                     IPGKEKWTLILNRQAKQWGAYFHKPEEDQLRFEVTPRPGAETEWMAFTLTPKGPDAAV
                     AEMDWAGLRVPFEIKVDVEKMVWANIDAALADNPGFDTYLTAYNYARDTGKRLDEAMG
                     WIDKSMALRESFWAHEAKARLLERQGQRAEALEHLEKAMTLAKGKAPQAYVDNLDKLK
                     AEWKAKG"
     gene            4154..>4493
                     /locus_tag="VEL74_19390"
     CDS             4154..>4493
                     /locus_tag="VEL74_19390"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYG64753.1"
                     /translation="MHEVERISRQIQQGFEGEAWHGPAVLEILADVDAALAAARPIPG
                     AHSIWELALHLAATQEIILRRIQGERAGLTEEEFWPATPEPTEEAWQATVARLRRQET
                     ELRQAVTEFPD"
BASE COUNT          624 a         1601 c         1545 g          723 t
ORIGIN      
        1 tggcggcggc ggctggagag cctgggctgg gcgagcctgg cgacggtctt cttcgacctc
       61 ttcctgatcc tcttccaggt gaaggcggtg tacgccaccc agctcggcga ttggagcgcc
      121 aaccactatg gaccggtggc gcgcaacctc tacggcctgg ggaggcacct gctggacctc
      181 cccttcaagc tggggctgcc gctcctgctc tgggcgggtt tctatctgga cgagctgccg
      241 ggccgatcgt gatccggccg tcgagagagg gatagggtga gcggggcttc cagaggcgcc
      301 gcggacggcg cctcctcagc ccctgacctc atgaatcctg ggtttcgtcc tcggactcgt
      361 catccccgtc atcatcgtcc tcgtcttcga gcagccccac cggctgatcc ggatcgactg
      421 gttgatccgg attctcgatg ggcgccccga gagggttctt cttccgttcc ttcttctggg
      481 ccagcttctc ttcccgcttc tgcttcttgg ccagatcgcg ctgccgctta ccatgctggt
      541 agttctgtcg tgccacgtat cgctcccata gtcggaccct gcgcgcccgg ggggtgcgtt
      601 gcttggctgg agctccatcc cggcgctagg ctgggccgga ggataccact atcggacagt
      661 caacgaggga cggccacctg cacgagttgc ggcgttcatc cgtaaaaagg atagacacgc
      721 tccctcgctg gcactggacg gagcctcgag gagacctcga tgaccggaca ccggcagcac
      781 cggcgacacc cggggggcag agccctgacg gccgccctcc tcggcctcct tctccttccc
      841 ggagcgctcg ctcccgctcc ggcgcccccg gatctcgagc cgccccgggc gctcacaccc
      901 cgcgggctcg ccaacctcat ggcgttctcg cgtctgctgg ggtacgtccg ctacttccat
      961 cccagcgatc aggccgccgc cgtcgattgg aacacgctgg ccatcgcggg cgtgcaggcc
     1021 gccgagccgg cggccgacgc ggtggcgctc gcgcgcacgc tggaggattt cttccacccc
     1081 atcgccccgg ccctgcgggt gttcgccggc ggaaggcgtc ccgagctccc ggccgagatc
     1141 ctgccgcccg ccgacacaga tccgacgaag accgcctggc atcacctggg cggcggcacc
     1201 ggcggcccgt tccacagcca ccggttccag cgctccatcg gagagcccgg caggctccgg
     1261 cagtcgctgg tcgcggcgcc gttgctgggc aagaaggtgc gcttcaaagc cgcggtccgt
     1321 gccgaggtgg agccccccca tgaggcgcgg ctctcgctgc tcgtcgatct tcccggagcg
     1381 gaggagcccc gggaggtgac cattccgtcc ggcggctggc agacgctcga gatcgtcgcc
     1441 gacatccccg ccgaggcccg cgacgtcgag gccggcgtcc tgctgctcca ccaggggcgc
     1501 gtctggatcg acggcgcggt gctcgaggtc gtcggagagg aaggagggaa cctgctgcgc
     1561 aaccccggtt tcgagcgcgg caacaccggc acgctgccgt tcggctggtc ggtgccgcgg
     1621 ggggcggacg aggcggggta cgtcgccgag atccgggagg agaggccggc ggaggggcgg
     1681 cgctgcgcgg cgctctcgtg ggtcgagccg gagccggtgt tccccaagcc gcacgagccc
     1741 ttcctcgcca acctgggagg cggggtggcg gccctggtgc cgatggcgct ctatgtcgac
     1801 gggcagggca cccggccgcc ggtgccgccg gaggtgacgc caccggcccc cgccaagccg
     1861 gcccgcttcg aaccctcggg ccaggaccgc gccacccgtc ttgccgccgt ggccctggcc
     1921 tggagcgccc tcgagcactt ctatcccttt ttcgaggaga ccgggaccga ctggaacggc
     1981 gcgctgcggc gggcgctcac cagcgcggcc accgatcccg gcgacgccgc cttcatccgc
     2041 acgctgcagc gtctggtggc cgcgctccag gacggtcagg cccgggtggc cggtccggcg
     2101 atcccgcaga ggttcctcct ccccatactc tgggactgga tcgagggaag gctggtggtc
     2161 acggcggtgc tgcccaagaa ggccggcgag atcgtccccg gcgacgtcat cctgagcctc
     2221 gatggccggc cggcggagga ggcgctggcc gccgccgagg agctgaccgc cgcggccacc
     2281 tccggcgccc ggcgcttcgc cgccgtccgt accctcctgg cgggcaatcc cttcgaggcg
     2341 gtgcaggtgg aggtccaacc cttccgtggc gagccgttct ccacgacgct gatccggtcg
     2401 atctctccgg aaggggacaa ggacctccag gagccgcggc cggagcccgt cgccgggctc
     2461 gaccgcggcg tcctctatct cgacctgatc cgcatggacc cggccgatgt ccgccgcgcc
     2521 atggctctga tccccgaggc gaccgggatc gtcttcgacg cccgtgcccc catgccgggg
     2581 ccgggcatgg acaacaacat ccgccggttc ctcccgctcc tgaccgtcga ctcgctggag
     2641 atgccgcggc gctccgttgc cgtcatcacc cgtcccgacc ggcgcgcctg gcaggattat
     2701 cccgtgtcgc acctctcacc ccagatgccg cgcttcgagg gggaggtcgc ctttctgatg
     2761 aacagcggcg tgatcggtga gctcgagtcc ttcctgaccc tggtggagca ctaccggatc
     2821 ggcgccctcg tcggctcccc caccgccgcc accatcggcg aggtgagctc catccgcctg
     2881 cccggcggct acgccgtcta ctggactgga acaaggatcc gcaagcaaga cggctcgcgc
     2941 caccacgccg tggggatcca gccccagctc gacgtggtgc gcagccgcgg cctcatcgcc
     3001 gctggccggg acgaggtcct ggaaaaggcc tgggcgctgg tggctccgcc ggtcgagcgg
     3061 caggaggagt agagagcggg atcagtcgag aagcgaagcg acgtcgagca ccagatccgg
     3121 aaatgtctcc ggcagcttca gctctcttcc ctcaatcagc tccacccgat cttcttctct
     3181 caccccttcg ccttccactc cgccttgagc ttgtccaggt tgtcgacgta ggcctggggg
     3241 gccttgccct tggcgagggt catggccttc tccaggtgct cgagggcctc ggcacgctgg
     3301 ccctggcgct cgaggagccg ggccttggcc tcgtgagccc agaagctctc gcgcagagcc
     3361 atcgacttgt cgatccagcc catggcctcg tccagacgct tgccggtgtc gcgggcgtag
     3421 ttgtaggcgg tcaggtaggt gtcgaagccg gggttgtccg ccagggcggc gtcgatgttc
     3481 gcccagacca tcttctccac gtccaccttg atctcgaagg ggacgcgcag gccggcccag
     3541 tccatctccg ccaccgcggc atccgggccc ttgggggtca gggtgaaggc catccactcc
     3601 gtctctgctc cgggccgggg cgtcacctcg aagcggagct ggtcctcttc gggcttgtgg
     3661 aagtaggcgc cccactgctt ggcctgccgg ttgaggatca gcgtccactt ctccttgccg
     3721 ggaatggcga agagggcata ggtgccggcc gggacctcct gccccgccac ctggaccggc
     3781 gtcgagaacg agagggtggt ggcggcgttg gctcccatcc gccagacctc gccgtagggg
     3841 acgaggccgc cccagatggt ccgccccttc accgcgggcc ggttgtagac gatctccacg
     3901 tccgtgatcc cgatcgtcag tcccaccttg gaggcggggc tcacccaggg gatgcggggc
     3961 gcctcggcct ggacggcggg cgtcaggagg agcagggaaa ggacgagggc gagcgcgaaa
     4021 tatgccttca tgggagacct ccgggagccg acggttggtt ctccatcatc ctaccggagg
     4081 cgatcccctc ttgtcttccc ggccccggag gtatgaaatc cccgggacgc aggaccgatg
     4141 acaggaggag acgatgcatg aagtcgagcg gatctccagg cagatccaac aggggttcga
     4201 gggcgaggcc tggcacgggc cggcggtgct ggagatcctc gcggacgtgg acgcggccct
     4261 cgccgccgcc cggccgatcc cgggagcgca cagcatttgg gagctggccc tccacctggc
     4321 cgcgacgcag gagatcatcc tgcgccgcat ccagggggag agggcgggtt tgaccgagga
     4381 ggagttctgg cccgccacgc cggagccgac cgaggaggcc tggcaggcga cggtcgcgcg
     4441 gctccggcgc caggagaccg aattgcggca ggccgtcacg gagttccccg acg
//
LOCUS       DASPJI010000684         4899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_442986, whole genome shotgun sequence.
ACCESSION   DASPJI010000684 DASPJI010000000
VERSION     DASPJI010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442986"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VEL74_19395"
     CDS             complement(<1..321)
                     /locus_tag="VEL74_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HYG64754.1"
                     /translation="MNWIFLGALLGALSVAGGAFGAHGLAQRLDPRGLELWETAARYL
                     MYGALALILLGLFGQQGARRGIDRAGWCLLLGSVIFSGTVAVLALGGPRWLGAVTPIG
                     GTLLI"
     gene            618..989
                     /locus_tag="VEL74_19400"
     CDS             618..989
                     /locus_tag="VEL74_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HYG64755.1"
                     /translation="MDLLSRARNLRYEMTDAERHLWSKLRRRFLHHKIRRQAPIGPYI
                     VDFACFGRKLVIEVDGGQHCGSAADAVRDEFLRGRGFLVLRFWNHEVLGNIEGVLEVI
                     AAALGGEEDGKEVVGKEEPPS"
     gene            complement(1207..1479)
                     /locus_tag="VEL74_19405"
     CDS             complement(1207..1479)
                     /locus_tag="VEL74_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64756.1"
                     /translation="MGTFHQGKSELHGITVIVDTHGPEVFVGRCDDMDERGILLNDVD
                     VHQDGEGGRSKQEYLKRAAMVGFWKKHDRLFIPTDKIASVKRLGEL"
     gene            complement(1491..1922)
                     /locus_tag="VEL74_19410"
     CDS             complement(1491..1922)
                     /locus_tag="VEL74_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014464955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HYG64757.1"
                     /translation="MPYSPLLVKPMREELSSIGVQELLTAQDVDAFMNEKNGTAMLIV
                     NSVCGCAAGMARPGVRLALQSDRRPDRVATVFAGQDLEATARARGYFSDVPPSSPSIA
                     LFKDGELVHFIPRHRIEGRDAYAVADDLKAAFNEHCPVPAS"
     gene            2053..2967
                     /locus_tag="VEL74_19415"
     CDS             2053..2967
                     /locus_tag="VEL74_19415"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HYG64758.1"
                     /translation="MNRIRSLAAVSLFALALLTGACRAADPEGSGEETAAAASPTPAP
                     SPAPVAPGPPPPAPSPVAAPAALPQDRPLRAGFLVVDGVYNSELMAPYDVFQHTTFHV
                     KPGIEVFTVSPDGQPVTTSEGLRLTPHHSFANAPAMDILVVPSTRGSMDADLRNQALI
                     DWVRRTAGTARHVVGLCDGAFVLAQAGLLDGVPATTFPEDYQRLSQMFPRVDLRVNVS
                     FVDAGKILTSQGGARSYDVAMHLVDQLYGPQVAQGVGRGLVIPWPPDPDTMVPRVVEH
                     PPTAPPASSPAASPAAPAPGTGAAPPPA"
     gene            complement(2915..3766)
                     /locus_tag="VEL74_19420"
     CDS             complement(2915..3766)
                     /locus_tag="VEL74_19420"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HYG64759.1"
                     /translation="MRRARRVQVQFWKQGEDHAYPGYTTNISTTGMFIATRSPLPQGT
                     RVRVEVLERDRGFMVEGVVAHALKVRGELMRISQPGMGIRFLSMEELVRELIPGVKWG
                     EEDIPAGAPASVRNAPPIPLFEPEPEPEPPPLPHAAPPPPPPPFPPPPIQRTAERSAP
                     PPPREPRDVPSGVGAYTVGFASPTEFLEVFQRDILQGGLFVPTRYPGRLHETLLIDLV
                     PPIPSAEPVRVRARVVQRFDPRSADLDGTNLLSGMGLELLDLPRVIEQLRPAVERLRS
                     QGLAQPG"
     gene            3826..>4899
                     /locus_tag="VEL74_19425"
     CDS             3826..>4899
                     /locus_tag="VEL74_19425"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HYG64760.1"
                     /translation="MPTLTPPLPRTMTVAACTPEGGVRLESRPLPEPGPGELRLRLRG
                     AGLCGTDLFKLRYGTAAEGMVLGHEVVGTVDALGPGTVGFLPGDRVVVPHHVACGECD
                     LCLRGSETLCPVFRENLLVPGGFAEHILVRERAVRLATFSLPGHLADEAAVFLEPAAC
                     VLRGLRHARLEETALPTSLEGHGPVAAVLGGGSMGLLHLLVARALFPDVRIVISDPLP
                     ERRALALRLGADAAAAPGQEVRQAVAGLSGGRGADVIFDTAGGARALDEALGLTREGG
                     AVVLFAHGAENETAGFGLNTFFKGERRLLATYSSSLADQRDIYRLLVSGRLDPSPLVT
                     HRLPLSRFGEAVSLARDQQALKVL"
BASE COUNT          674 a         1781 c         1727 g          717 t
ORIGIN      
        1 gatcagcagc gtgccgccga tcggggtgac cgccccgagc caccgcggcc cgcccagggc
       61 gaggacggcg acggtgccgc tgaagatgac gctcccgaga aggaggcacc accccgcgcg
      121 gtcgatcccc cgccgcgccc cctgctgccc gaacagcccg aggaggatca gcgccagggc
      181 gccatacatg agataccgcg ccgcggtctc ccacaattcc agcccgcggg gatccagccg
      241 ctgcgcgagc ccatgagcgc cgaacgcccc gcctgccacc gagagggcgc cgaggagggc
      301 gcccaggaag atccagttca ttgtgttccc ctccggggag gcattctatg gtgggggctg
      361 ggctgggagg aaagaaaacc cccctcctga cctcccccgc tccctcccgc ccacccgccc
      421 agggggggag gcaccaccgc cctctcacgg cacaacgggt caggctacac aggaggagga
      481 cttcgaggag tcctcctccc ttgcctatcc aagggggagg aggtgcctcc ccccctgggc
      541 gggggcgtgg gagggagcgg gggaggtcag gaggggggtt cttcttcttt cctgttcctg
      601 tatcctccac ccacgccatg gacctcctct cccgggccag aaatctccgt tacgagatga
      661 ccgacgccga aaggcatcta tggagcaagc tccgccgccg cttcctgcac cacaagatcc
      721 gccgccaagc cccgatcgga ccctacatcg tggacttcgc ctgcttcggg aggaagttgg
      781 tgatcgaggt cgatggcggc cagcactgcg ggagcgcggc cgatgccgtt cgggacgagt
      841 tcctgcgggg gagagggttt ctcgtccttc gcttctggaa ccacgaggtt ctcggcaaca
      901 tcgagggagt gctggaggtg atcgcggcag cgttgggggg agaagaggac gggaaggaag
      961 tggtgggaaa agaagaaccc ccctcctgac ctcccccgct ccctcccgcc caccctccca
     1021 gggggggagg cacctcctcc cccttggata ggagagggaa gaggactcct cgaagtcctc
     1081 ctcctgcgtg acctgacccg ttgtgccgcg aggggcggtg gtgcctcccc ccctgggcgg
     1141 cggcttggga gggagcgggg gaggtcagga ggggggttct cctcctctcc cctacttccc
     1201 ctcccgctac agctcgccca accgcttcac cgaggcgatc ttgtcggtgg ggatgaagag
     1261 ccggtcgtgc ttcttccaga agccgaccat ggcggcccgt ttcagatact cctgcttgga
     1321 gcggccgccc tccccgtcct ggtggacgtc gacgtcgttg agcaggatgc cgcgctcgtc
     1381 catgtcgtcg cagcggccga cgaacacctc cggaccgtgg gtgtccacga tcacggtgat
     1441 gccgtgcagc tcgctcttgc cctggtgaaa ggtgcccatt cagctcgccc ctagctggca
     1501 ggcactgggc agtgctcgtt gaaggccgcc ttgaggtcgt cggccacggc gtaggcgtcg
     1561 cggccctcga tgcggtggcg cgggatgaag tggaccagct cgccgtcctt gaagagggcg
     1621 atcgacgggc tgctcggggg aacgtccgag aagtagccgc gggcgcgggc cgtcgcctcg
     1681 aggtcctggc cggcgaacac ggtggcgacg cggtcgggtc ggcggtccga ctggagcgcc
     1741 aggcgcacgc ccggacgggc catgccggcg gcgcagccgc agaccgagtt gacgatcagc
     1801 atcgcggtgc cgttcttctc gttcatgaac gcgtcgacgt cctgggccgt caacaattcc
     1861 tgcacaccga tgctcgagag ctcttcgcgc atcggcttga ccagtagcgg gctatagggc
     1921 acttggcacc tcctgtaggg gatcgagggc cggctggccc ccgaagatca ctgtcctatt
     1981 ctacacaggc acgccggccg ctattcccgg gtcccccccg gatggccggc ttcttgctca
     2041 cgggagacga tcatgaaccg aatccgctcc ctcgccgctg tttcgttgtt cgcgctcgcg
     2101 ctgctcaccg gcgcctgccg cgccgccgac ccggagggct ccggggagga aaccgccgcc
     2161 gccgcgtccc cgaccccggc tccctccccg gcaccggtgg ccccgggccc gccgccgccg
     2221 gctccctcgc cggtcgccgc gccggccgcc ctcccccagg accggccgct gcgcgccggc
     2281 ttcctggtgg tcgacggcgt ctacaactcg gagctgatgg ccccgtacga cgtgttccag
     2341 cacaccacct tccacgtgaa gccggggatc gaggtcttca ccgtgtcgcc ggacggccag
     2401 ccggtcacca cctccgaggg gctgcggctc acgccgcacc actccttcgc caacgccccg
     2461 gccatggaca tcctggtggt gcccagcacc cgcggcagca tggacgccga cctccggaac
     2521 caggcgctga tcgactgggt gcgccggacg gcgggcacgg cgcggcacgt ggtcggcctc
     2581 tgtgacggcg ccttcgtgct cgcccaggcc ggcctgctcg acggcgtccc ggccaccacc
     2641 ttccccgagg actaccagcg cctgtcgcag atgttcccgc gggtggacct gcgggtgaac
     2701 gtcagcttcg tcgacgccgg caagatcctc acctcccagg ggggagcccg gagctacgac
     2761 gtggcgatgc acctggtgga tcagctctat ggcccgcagg tggcgcaggg agtcggccgg
     2821 gggctggtga tcccctggcc gccggacccg gacaccatgg tcccgcgggt ggtcgagcac
     2881 ccgccgacgg caccgccggc atccagcccc gcggctagcc cggctgcgcc agcccctggg
     2941 accggagccg ctccaccgcc ggcctgagct gctcgatgac tcgcggcagg tcgaggagct
     3001 ccaaccccat gccggagagg aggttggtgc cgtccaggtc ggcggaccgg gggtcgaaac
     3061 gctggaccac ccgggcgcgg acgcgcaccg gctcggccga ggggatcggc ggcacgaggt
     3121 cgatcagcag cgtctcgtgc agacggccgg gatagcgggt cggcacgaac agcccgccct
     3181 gcaggatgtc cctctgaaag acctcgagga actccgtggg gctggcgaag cccacggtat
     3241 aggcgccgac gccgctcggc acgtcccggg gctcccgggg cggcggcggg gcgctccgct
     3301 cggcggtccg ctggatcggg ggaggtggga acggaggggg aggcggcgga ggcgcggcat
     3361 ggggaagggg cggcggctcc ggctcgggct ccggctcgaa gagggggatg ggaggggcgt
     3421 tgcggaccga ggccggggcc cccgcgggga tgtcctcctc cccccacttc actccgggta
     3481 tcagctcgcg gaccagctcc tccatcgaga ggaagcggat ccccatccct ggctggctga
     3541 tccgcatcag ctcgccgcgg accttgaggg cgtgcgccac caccccctcc accatgaagc
     3601 cgcggtcgcg ctccagcacc tcgacgcgca cccgcgtccc ctgcgggagg gggctgcggg
     3661 tggcgatgaa catgccggtg gtcgagatgt tggtcgtgta gccggggtag gcatggtcct
     3721 ccccctgctt ccagaactgc acctggacgc ggcgggctcg gcgcacactg cggcggcggg
     3781 tcactacgtc attataggat gtgaccgacg agacgcagcc gcgagatgcc gacgctgacc
     3841 cctcccctgc cccggaccat gaccgtcgcc gcctgcaccc cggagggggg cgtgcggctg
     3901 gagagccgtc cgctgccgga gcccggtccc ggggagctgc gcctccgcct gcgcggcgcc
     3961 ggcctgtgcg gcaccgacct gttcaagctg cgctacggca cggccgccga ggggatggtg
     4021 ctcgggcacg aggtggtggg gacggtcgac gccctgggac ccggcaccgt cggcttcctc
     4081 cccggcgacc gggtcgtcgt cccccatcac gtggcctgcg gtgagtgcga cctctgcctg
     4141 cgcggcagcg agacgctctg ccccgtgttc cgggagaacc tcctggtgcc cggcggcttc
     4201 gccgagcaca tcctggtgcg ggagcgggcg gtgcgcctgg ccaccttcag cctgcccggc
     4261 cacctcgcgg acgaggccgc cgtcttcctg gagcccgccg cctgcgtgct gcgcggcctc
     4321 cgccacgcgc ggctggagga gaccgccctg cccacctctc ttgagggaca cgggccggtg
     4381 gccgccgtcc tcgggggagg cagcatgggg ctcctccacc tcctggtcgc gagggccctg
     4441 ttccccgacg tgcggatcgt gatctcggac ccgctgccgg agcgccgggc cctggccctc
     4501 cgtctcggag ccgacgccgc cgccgccccc ggccaggagg tccggcaggc ggtggccggg
     4561 ctctccggcg gccgcggtgc cgacgtgatc ttcgacacgg ccggcggcgc ccgggcgctg
     4621 gacgaggccc tggggctcac ccgcgagggc ggggccgtcg tcctcttcgc ccatggagcg
     4681 gagaacgaga ccgccggctt cggcctcaac accttcttca agggggagcg gcggctgctc
     4741 gccacctact ccagctcgct cgccgaccaa cgggacatct atcggctgct ggtgagcggc
     4801 cggctcgatc cctcccccct ggtgacccac cggctgccgc tgtcgcgctt cggcgaggcg
     4861 gtgtccctgg cccgcgatca acaggccctg aaagtgctg
//
LOCUS       DASPJI010000685        11733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_390997, whole genome shotgun sequence.
ACCESSION   DASPJI010000685 DASPJI010000000
VERSION     DASPJI010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11733)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11733
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390997"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3092
                     /locus_tag="VEL74_19430"
     CDS             <1..3092
                     /locus_tag="VEL74_19430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64761.1"
                     /translation="VRRHEVLRSVFRESDGEPVQVARPPASVPLPVADLSALPPVDRR
                     REVQRLAQGEAERPFVLSRGPLLRALLAVLDRTDHAVLLTLHHIVSDGWSTGILVREL
                     GTLYAAFSRGERSPLAEPEIQYADFAGWQRSWLTGEVLEGEIDYWRRRLEGAPPALDL
                     PADRPRPPVQTQRGAAVTFAIPAELMRELRALGRRQGATLFMTLLAAFQTLLARSTGQ
                     LDVNVGTLVAGRSQLRTEDLIGFFINTLVLRSDLSGEPAFSELLSQVRGVTLEAFAHQ
                     DLPFERLVEELQPARDLSRTPLFQVLFALQNAPVGALEVPGMRLEPLLAERRTSLFDL
                     ALTLEDGGIGSAEYSTDLFAAATIERLMGNLVALLEGAVAAPESAVGDLPLLRAAERH
                     QIVREWNDTARVWPRQLRLHDLIVEQARRAPDALALVQGGDHLTYGELLHRAAGLARS
                     LRALGAGPEVTVALCLERSLEMGVALLGSLLSGATWVPLEPTYPPERLSFMLEDSGAA
                     VLLTQERLLATLPSGSFATICLDRELADAPREAAADQAAPESAAYVIYTSGSTGRPKG
                     VVVSHEALVSYALEVVRFCRLGPADRVLQFAPLGFDVIVEEVFPIWVAGGAVVLEPAE
                     RLASPLDLWAALDETAVTVMELPTAYFHELVYGLEVSGGRLPASLRLVIVASEKLLPE
                     RVVSWHRHGVDLLHVYGLTEATVTSSYHLLPAGAELDDPDLPIGRPMADTSLLILDPG
                     LWPVPAGVAGELCVGGVGLARGYLGRPELTAERFVPDPFSDLPGARLYRTGDLARLRS
                     DGNLHFLGRVDFQAKVRGFRIEPGEIESRLAEHPGVREVLVAVREDRPGDKRLVAYLV
                     ATALEGEAEPPGGGELRGFLRETLPDYMVPSAFVFLEALPLTPNGKVDRRRLPVPPDE
                     RPGTLLYTAPRNAAEEVLARIWSELLGRDRIGIFDDFFELGGHSLLAARTTARLRSVF
                     GLELPLRALFEAPTVAGLGEALARLAGEPGIVDQIAQVYLEVEGLSEDEMRGILSES"
     gene            3108..>11733
                     /locus_tag="VEL74_19435"
     CDS             3108..>11733
                     /locus_tag="VEL74_19435"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64762.1"
                     /translation="MSRSDAGQMERSRVFESLLRKRGIQAPRATAIPRRGKEGPCVLS
                     FAQERLWLLDQMAPGGSTYNMPTALRLDGTLDVPALARSLSEVARRHGALRTRFPVRD
                     GVPVQDVAPPVSVPLPMADLSGLRGAVRDREAWRLVGEAVRRPFGLASGPPFRAQLLR
                     FASADHVALLTVHHIAGDGWSMEILVRELAILYGSFARGSASPLPEPPIQYTDFAVWQ
                     RTRLTGELLALELEFWRERLAGAPRLLELPSDRPRPAVKTFRGALQSWTLPAGLARDL
                     REVGQRHGTTLFVTLLAGFQALLTRLSGQPDVLVGTPVAGRGRSELEGVIGVFINTLV
                     LRADLSGDPSFAELLERVREASLAAQEHQELPFERLVEELAPERSLSHPPLVQVMFAL
                     QNAAPGGQPTLEGVRMSPLEPPHETSKLDLALTLGEGAEELSAAFEYSTDLFDTTTVA
                     RWAGHLERLLAGAVAAPAARLSELPWLAPAERAQVLMEWNDTGMAAAGEALPDRVAAQ
                     ARRAPDAVAVTAGDLSVSSGFLDQWAGRLAGRLRRRGAGAESVVGLQLPRSPEMVAAM
                     LAVWRSGGAYLPLDSSLPAFRTEALLADARALLLSHEEVWLSDGEETVPVEGVHAGGL
                     AYVIYTSGSTGRPKGVAVSHGALDNLVDWHLREYGVTPADRASQVAGLGFDASVWEIW
                     PYLVAGASVHLLPEEVEPEPVRVAGWLAEAGITHCFLPTPLAEEVLVGKMGRLPALRH
                     LLTGGDRLHRAPSEDPGFHLINHYGPTEGAVVATAGLVATAPHDPRPPAIGRPIANAA
                     IRLLDRDLRPVPVGVAGEMYLAGAGLARGYLHRPDLTAERFLPDPAAAQSGRRVYRTG
                     DLARWSPRGELEFLGRADFQVKVRGFRIETGEIEAALGLHPRVREVAVVPRDGHAGRH
                     LVAYVVRAAGDPLDTGELRAFLRERLPEYMVPSAFVDLPALPLTPNGKVDRAALPDPP
                     EETGQGSVPPRDPVEEVIAALWADALGRERVGVFDGFFELGGHSLLAARVIARVRSTF
                     GVDLSLADLFAEPTVAGLAARVAAALRAGGGTAAPLAPAPRDRPLPQSFAQQRLWFLD
                     RLEPGSPAYNVPLHLRLAGELSVQALAGALAEVARRHEVLRTTFDSVAGEPVQIVGPP
                     PAPALPVVDLTALTPPARVAEAGRLADRESLRPFDLRRGPVFRVTLLRLAAEDHVLLA
                     TLHHIAADAWSVEVLRREIAALYTALHSPLREGRPSPLPELPVQYGDFAVWQRRVLES
                     GALEGQMEYWRGRLGSEPAVLELPTDRPRPAVQTYRGAHLPFTLPPEVAAGLLALGRR
                     AGATPFMAALAVFQGLLRRITGQRDVSVGTPVSGRHHVELEALIGFFLNTLVIRVDLP
                     GDPVFAGLLERVRDLCLEAYAHQDLPFERLVDELQPRRSLSHSPLFQVLFVLQHEAPV
                     SAAGEPGWSPWISEPAVAKFDLTLSLAVGERGIQGSLEYNTDLFDVSTAARLVAQLER
                     LAGAAADASGRPLSVLPILSAAESQQLLLEWNDAGGPLAADPALQRLVEEQARRTPDA
                     VAVTLGEAALSYAGLDHRAGRLARHLRALGVGPDARVGLAVERSLEMAVGLLAIFKAG
                     GAYVPLDPAYPRERLGLMLEDAGVEVLVTQAGLLESSDEATLRVFRLDADWEAVPAEP
                     LEPDNAGAALTGEELAYVIFTSGSTGRPKAVALSHRALRNLIDWHLATLVGGARTLQF
                     ASLSFDASFHEMFACWGSGGTLHLVSEEVRRNVPALAALLLEAGIEKAILPVVVLHQL
                     AEELGRRDGLPPLLEVTTTGEQLQTTAAMAELFRRLPGCAFHNHYGPSESHVATAYTL
                     SPGPAAWGTHPSIGLPIWNSSVHLLGRDHESVPIGVPGDLYIGGVCLARGYLGRPDLT
                     ADRFVPDPFAGEPGARLYRTGDKVRRLPDGNLDFLGRFDHQVKVRGFRVELEEVESVL
                     GRHPGVRQTVVLARGEGAVDKSLVAYVVPEPTVSGSAAGAVEWRAFLRERLPDYMVPG
                     HFVVLEALPLNANGKVDRAALPAPAADRPDLVSPFVAPRNPSEELVAAVWADLLGIER
                     IGVHDDFFALGGHSLMATRVVSRLRDAFHVELPLRGLFEEPTVAGLARRLDEAVRAGA
                     GLAAPPIVPVPRGDGEIELPLSFAQQRLWFIEQLEPGSSHYNVASPLRLRGLLRVEAL
                     AGALTGIVRRHQVLRTSFPHTGGRPVQAVAAEWRTGLPVLDLSSLPEEVRQREASHLA
                     GEEARRPFDLAAGPLLRTSLVRLGPAEHVLLLCVHHIVWDGWSEGIFVRELAALYGTL
                     AAGRPGTLPELPVQYADFACWQRRWMSGEVLERQLAYWRQRLAGAPAHLDLPADRQRP
                     AAPAFRGGALPFALPEPASAAVAALGRRHGATLFMTLAAAVDALLHRWTGAEDVVIGT
                     PVAGRDRLEVEGLIGFFVNSLVLRTDLSGDPKFPLLIERARDGALGAYAHQDLPFERL
                     VEELAPDRSRPHSPLFQVLLALQNAPAAALDLPGIELEPLELERRSTRFDLTLSLSEG
                     REGISGTLVYSADLFDAATAERMLGHLGVLLEGIAEEPARRLSELPVLTAAERRQLLE
                     EWNQGRGVAEEEGGLFDVFAARVAEAPDGVAVTCEGRALSYGALRERAEGLADGLRHL
                     GVGPESRVGLCIERSEEMIVGILGILAAGGMYVPVDPEQPQERLELILRDALSGTAAP
                     VVVTSGGLAEKVGREMAGQGIRRVGVGETVEGSGGGPCSAPSGAPPLPPSQRVQSPAP
                     VLPRSPRRPRDVGAYVIYTSGSTGVPKGVVVSQGNVLRLFEATAGRFRFGRGDVWTLF
                     HSFAFDFSVWEIWGALMYGGRLVVVPQEVVRSPGEFHRLLVREGVTV"
BASE COUNT         1380 a         4118 c         4359 g         1876 t
ORIGIN      
        1 tcgtgcggcg ccacgaggtg ctgcgcagcg ttttccggga atccgatggc gagccggtcc
       61 aggtggcgcg gcctccagcg tccgtcccct tgccggtggc ggacctctcc gcgctgccac
      121 cggtggaccg gcggcgggag gtccagcggc tggctcaggg agaggccgag cgccccttcg
      181 tcctctcacg gggaccgctg ctgcgcgccc tgctcgcggt cctcgaccgg acggaccatg
      241 cagtcctcct gacgctgcat cacatcgtca gcgacggctg gtccaccggc atcctcgtgc
      301 gcgagctggg taccctgtac gccgccttct cccgcggcga acgctcgccg ctggccgagc
      361 ccgagatcca gtacgccgac ttcgccggct ggcaacggag ctggctgacc ggcgaggtgc
      421 tggaggggga gatcgactat tggcgccgcc gcctcgaggg ggcgccgccg gcgctcgacc
      481 tgcccgcgga ccggccgcgc cctccggtgc agacgcagcg aggcgcggcg gtcacgttcg
      541 ccatcccggc ggagctgatg cgggagctcc gcgccctggg ccggcggcaa ggggcgaccc
      601 tgttcatgac gctcctggca gcgttccaga ctctcctcgc ccggtcgacg gggcagctcg
      661 acgtcaacgt cggcaccctc gtcgccggcc gcagccagct ccgcaccgag gatctcatcg
      721 gcttcttcat caacaccctg gtcctgcgct ccgacctgtc cggcgagcca gccttctccg
      781 agctgctctc ccaggtgcgc ggggtgaccc tcgaggcctt cgcccatcag gacctcccct
      841 tcgagcggct ggtcgaggag ctgcagccgg cgcgggacct ctccagaacc ccgctcttcc
      901 aggtcctctt cgcgttgcag aacgcgcccg tgggcgccct cgaggtgccg gggatgcgcc
      961 tggagccact cctggccgag cgccggacct ccctcttcga cctcgccctg accctggaag
     1021 acggggggat cggttcggcc gagtacagca ccgacctctt cgccgccgcc acgatcgagc
     1081 gcctcatggg aaatctggtg gcgctgctcg agggcgcggt ggcggcgccc gagtccgccg
     1141 tgggggatct ccccctgctg cgcgccgccg agcggcacca gatcgtccgc gagtggaacg
     1201 acaccgcccg cgtctggccc cgccagctcc gtctgcacga tctgatcgtg gagcaggccc
     1261 gccgtgctcc ggacgcgctg gccctggtgc agggtgggga tcatctgacc tatggtgagc
     1321 tgctccaccg cgccgccggg ctcgcccgca gcctgcgcgc tctaggagcg ggtccggagg
     1381 tgacggtcgc cctctgcctg gagcgctcgc tggagatggg ggtggcgctc ttgggctccc
     1441 tgctgtccgg ggcgacctgg gtacccctgg agcccaccta tccgccggag cggctgtctt
     1501 tcatgctgga ggactcgggc gccgcggtgc ttctcaccca ggagcgcctg ctggccactc
     1561 ttccgagcgg cagcttcgcg accatctgcc tggaccgcga gctcgccgac gcgccgcggg
     1621 aggcggcggc ggaccaggca gcgccggaga gcgccgcgta cgtcatctat acatcgggct
     1681 cgacgggacg tcccaagggc gtcgtcgtgt cccacgaggc gctggtgagc tatgccctgg
     1741 aggtggtccg cttctgccgc ctgggaccgg ccgaccgggt cctgcagttc gcccccctgg
     1801 gcttcgacgt catcgtcgag gaggtgttcc ccatctgggt ggcgggcggc gcggtggtgc
     1861 tggagccggc cgagcggctg gcgtcgccgc tggacctctg ggcagccctc gatgagacgg
     1921 cggtcaccgt catggagctg ccgaccgcct acttccacga gctggtctac ggcctggagg
     1981 tctcgggcgg gcggctcccc gcgtctctgc gcctggtgat cgtggcctcc gaaaagctgc
     2041 tgccggagcg ggtggtctcc tggcaccgcc acggcgtgga tctgctgcac gtctacggcc
     2101 tgaccgaggc gacggtcacc tccagctacc acctgctgcc ggcgggcgcg gagctggacg
     2161 atcccgacct gcccatcggc cgccccatgg cggacacctc gctcctgatc ctcgatcccg
     2221 gcttgtggcc cgtgccggcg ggcgtggcgg gggagctgtg cgtgggcggc gtcggcttgg
     2281 cgcgcggcta tctcggacgg ccggagctga ccgccgagcg cttcgtgccc gatcccttct
     2341 cggacctgcc cggcgcccgg ctgtaccgga ccggcgacct cgctcgcctg cggtccgacg
     2401 gcaatcttca tttcctgggg cgggtcgatt tccaagccaa ggtgcggggt ttccgcatcg
     2461 agccggggga gatcgagagc cgcctggccg agcacccggg cgtgcgcgag gtgctcgtgg
     2521 cggtgcggga ggaccggccc ggagacaagc ggctggtcgc ctatctggtg gccacggcgc
     2581 tggagggcga ggccgagcca ccgggcggcg gcgagctgcg cggcttcctg cgcgagaccc
     2641 tgccggacta catggtgccg tccgccttcg tctttctgga ggccctgcct ctcaccccca
     2701 acggcaaggt ggaccgccgc cgcctgccgg tcccgcctga cgagcggccc ggcactcttc
     2761 tctacacggc accgcgaaat gccgccgagg aggtgctggc gcggatctgg tcggagctgc
     2821 tggggcggga ccggatcggc atcttcgacg acttcttcga gctgggtggg cactcgctgc
     2881 tggcggcgag gaccacggcc aggctgcgca gcgtcttcgg cttggagctg ccgctacgcg
     2941 ccctgttcga ggccccgacc gtggcggggc tgggggaggc gctcgcccgc cttgcggggg
     3001 agcccggcat cgtcgaccag atcgcccagg tgtacctgga ggtcgaaggc ctttccgagg
     3061 atgagatgag ggggatcctc tccgagagct gaagcgagga atggacgatg agccgtagcg
     3121 acgcgggaca gatggagcgg tccagggtct tcgaatccct gctccgcaag cggggcatcc
     3181 aggccccgcg ggcgaccgcc atcccgcggc ggggcaaaga ggggccttgc gtcctctcct
     3241 ttgcccagga gcggctctgg ctgctcgacc agatggcgcc cggcggctcc acctacaaca
     3301 tgccgacagc cctgcggctc gatggcacgc tggacgtgcc ggcgctcgcc cgctcccttt
     3361 ccgaggtggc gcggcggcat ggcgcgctcc gcacccgctt cccggtccgc gatggcgtcc
     3421 cggtgcagga tgtggcgccg cccgtgtccg tgcccctgcc aatggccgat ctttcggggc
     3481 tgcgtggagc ggtgcgagac cgcgaggcgt ggcgccttgt gggagaggcg gtgcgccggc
     3541 ccttcggcct ggcgtccggt ccaccgttcc gggcccagct gctgcggttc gcttcggcgg
     3601 accacgtggc cctgctgacg gtgcaccaca tcgcgggtga cggctggtcg atggagatcc
     3661 tggtgcgcga gctcgccatt ctctacggga gcttcgcccg agggagcgcc tcgccgcttc
     3721 ccgagccgcc gatccaatac accgacttcg ccgtctggca gcgcacccgg ctcaccggcg
     3781 agctcctggc gcttgagctc gaattctggc gcgagcggct cgccggcgcg ccgcggctgc
     3841 tggagctgcc gtccgaccgg ccgcgccccg ccgtgaagac gttccggggg gcgctgcaga
     3901 gctggacgct gcccgccggg ctcgcccgtg acttgcggga ggtggggcag cggcacggga
     3961 cgactctgtt cgtgactctc ctcgccggct tccaggcgct cctcacgcgg ctgagcggtc
     4021 agcccgatgt gctcgtcggt acgccggtcg ccggccgcgg gcgctccgag ctggaagggg
     4081 tgatcggggt cttcatcaat actctggtcc tgcgcgccga cctatcgggg gacccgtcct
     4141 tcgcggagct cctcgagcgg gtgcgggagg cgtcactggc ggcccaggag caccaggagc
     4201 tgccgttcga gcggctggtg gaggagctgg cgccggagcg gagcctctcc catccgcctc
     4261 tcgtgcaggt gatgttcgcc ctccagaacg ccgctcccgg cgggcagccg acgctggagg
     4321 gggtgcggat gagcccgctc gaaccgccgc acgagacctc caagctcgat ctggcgctga
     4381 cgctggggga gggggcggag gagctgtcgg cggcgttcga gtacagcacc gatcttttcg
     4441 acaccacgac ggtggcgcgc tgggccgggc atctcgagcg gctcctggcc ggggccgtgg
     4501 ccgccccggc ggcgcggctg tcggagttgc cctggcttgc tccggccgag cgcgcccagg
     4561 ttctcatgga gtggaacgac accgggatgg ccgccgccgg ggaggcgttg ccagaccgtg
     4621 tggcggcgca ggcccgacgt gcccccgacg ccgtggctgt caccgcgggc gacctttccg
     4681 tttcctctgg ctttctcgac caatgggccg gccggctggc cgggcgcctg cgtcgtcggg
     4741 gagccggggc cgagtccgtg gtggggctcc agctcccgcg ctcgccggag atggtggcgg
     4801 ccatgctcgc cgtctggagg tctggagggg cgtacctgcc gctggattcc agcctgccgg
     4861 cgttccgcac cgaagcgctt ctggccgacg ctcgagccct gctgttgagc cacgaggagg
     4921 tctggctgtc ggatggcgaa gagacggtgc cggtggaggg cgtccatgcc gggggcttgg
     4981 cttacgtgat ctacacctcg ggatcgacgg gccggcccaa gggggtcgcg gtctcccacg
     5041 gcgctctgga caacttggtg gactggcatc tgcgggagta cggagtcaca ccggccgacc
     5101 gggcgtcgca ggtggcgggt ctcggcttcg atgcctcggt gtgggagatc tggccctacc
     5161 tggttgccgg tgcctcggtc cacctcctgc ctgaagaggt cgagccggag ccggtccgtg
     5221 tggccggctg gctcgccgaa gcgggcatca cccactgttt cctcccgact ccgctggcgg
     5281 aggaggtcct ggtgggcaag atggggaggc tcccggcgct ccgtcacctg ctcaccggcg
     5341 gcgaccggct gcaccgggct ccgtcggagg acccgggctt ccatctgatc aaccactacg
     5401 ggcctacgga gggagccgtg gtggccaccg ccggtctggt ggccacggcg ccgcacgatc
     5461 cgcgcccgcc cgccatcggc cggccgatcg ccaacgcggc gatccgcctc ctcgaccggg
     5521 acctgcggcc ggtgccggtg ggtgtcgccg gcgagatgta cctggccggc gcggggctcg
     5581 cccggggtta cctgcaccgt ccggacctga cggccgagcg ctttctcccc gatccggctg
     5641 ccgcccagtc cgggcgccgc gtctacagga cgggagatct ggcccgctgg tcccctcgag
     5701 gggagctgga gttcctcggc cgcgctgact tccaggtcaa ggtgcggggc ttccgcatcg
     5761 agaccggaga gatcgaggcc gccctcgggc ttcatccccg agttcgggag gtggcggtgg
     5821 tgccgcggga cgggcatgcc ggccgccacc tggtcgccta tgtggtgcgc gcggccggag
     5881 atccgctgga caccggcgag ctgagagcct tcctgcgcga gcggctgccc gagtacatgg
     5941 tgccgtcagc cttcgtcgac ctcccggcgc tccccctcac ccccaatggc aaggtggaca
     6001 gggcggcact gccggacccg ccggaggaga ctgggcaagg ttcggtgccg ccgcgcgatc
     6061 ctgtggagga ggtgatcgcg gctctttggg cggacgcgct gggccgcgag cgggtagggg
     6121 tcttcgatgg gttcttcgaa ctcggcgggc actcccttct ggcggcccgg gtcattgcgc
     6181 gggtgaggag cacgttcggg gtcgacctga gcctcgcgga tctcttcgcc gagcccaccg
     6241 tggccggcct cgcggcccgc gtggcggcgg cgctacgggc tggaggagga acagcggccc
     6301 cgctcgcgcc cgcaccccgc gaccggccgc tcccccagtc cttcgcgcag cagaggctct
     6361 ggttcctgga ccgcctggag cccggcagcc cggcctacaa cgtgcccctc cacctgcggc
     6421 tggccgggga gctgtccgtg caggctctcg ccggtgccct ggccgaggtg gcgagacggc
     6481 acgaggtcct gcgcaccacc ttcgactcgg tggccggcga gccggtgcag atcgtgggac
     6541 cgccgcccgc tccggccctg cccgtcgtgg acctgacggc gctgacgccg ccggcacggg
     6601 tggccgaggc cggacgtctg gcggaccgcg agtcgctccg cccgttcgac ctgcggcgtg
     6661 ggccggtctt ccgggtgacc ctgctgcggt tggcggcgga ggatcacgtg ctcctcgcca
     6721 cgctgcacca catcgccgcc gacgcctggt ccgtggaggt gctgcgccgg gagatcgctg
     6781 ccctctacac agccctgcat tcccccctgc gcgagggccg gccctcgccc ctgccggagc
     6841 tgcccgtgca atacggcgac ttcgccgtct ggcagcggcg cgtcctggag tccggtgccc
     6901 tggaagggca gatggagtac tggcgcgggc ggttggggag cgagcccgcc gtgctggagc
     6961 tgcctacgga ccggccgcgc ccggccgtcc agacctaccg cggcgcccat ctcccgttca
     7021 ccctgccgcc ggaggtcgcc gcgggtctgc tcgccctggg ccgccgggcc ggcgcgacgc
     7081 cgttcatggc cgccctggcc gtcttccagg gactgctgag acggatcacc ggacagcggg
     7141 acgtgagcgt gggcacgccg gtgtccgggc gtcaccacgt ggagctggaa gcgctcatcg
     7201 gcttcttcct caataccctg gtgatccgcg tcgacctgcc cggcgatccg gtgttcgccg
     7261 gcctgttgga gcgggtgagg gacctctgcc tggaggccta tgcccaccag gacctcccgt
     7321 tcgagcgcct agtggacgag ctccagccgc ggcgcagcct cagccactca ccgctcttcc
     7381 aggtgctctt cgtcctccag cacgaggcgc cggtctccgc cgcgggggag ccgggttgga
     7441 gcccctggat ctctgagccg gccgtggcga agttcgacct gacgctgtcg ctcgccgtgg
     7501 gagagcgggg gatccaggga tctctcgagt acaacacgga cctcttcgac gtctccacgg
     7561 ccgctcgcct ggtggcgcag ctcgagcgtc tcgccggcgc cgcggcggat gcgtccggcc
     7621 ggccgctctc tgtcctgccg atcctgagcg ccgccgagag ccagcagctc ctgctggagt
     7681 ggaacgatgc cggcggcccc ctggccgccg atccggcgct ccagcgtctg gtggaggagc
     7741 aggcccgccg cacgccggac gcggtggccg tcaccttggg cgaagcggcg ctgagctatg
     7801 ccggcctcga ccaccgggcg ggccgtctgg cgcgtcatct ccgtgctctc ggagtggggc
     7861 cggacgcgcg ggtgggcctg gcggtggagc gctcgctgga gatggcggtc ggcctgctgg
     7921 cgattttcaa ggcaggcgga gcctacgtcc ccctagaccc ggcctatcca cgggagcggc
     7981 tcggcctcat gctggaggac gccggcgtcg aggtgctggt gacccaggcc ggccttctgg
     8041 agagctccga tgaagccact ctccgggtct tccggctgga cgcggactgg gaagccgtcc
     8101 ccgcggagcc cctggagcct gacaacgccg gcgccgccct gaccggtgag gagctggcct
     8161 acgtcatctt cacctcgggc tctaccgggc ggcccaaggc cgtggccctc tctcatcggg
     8221 cgctgcgcaa cctcatcgac tggcacctgg cgacgctcgt gggaggcgcc cggacgttgc
     8281 agttcgcctc cctgagcttc gatgccagct tccacgagat gttcgcctgt tggggctccg
     8341 gcggcaccct ccacctcgtc tccgaggagg tgcggcggaa tgtcccggct ctagccgcct
     8401 tgttgctgga ggccggcatc gagaaggcga tcctgccggt ggtcgtcctg caccagctcg
     8461 ccgaggagct cggccggcgc gacggcctgc cgccactgct cgaggtcacc accaccggcg
     8521 agcagctcca gaccacggcg gccatggcgg agctgttccg gcgcctcccg ggctgcgcct
     8581 tccacaatca ctacgggccg tccgagagcc acgtggccac ggcgtacacc ctgagcccgg
     8641 gcccggcggc ctgggggacc cacccctcga tcggcctgcc gatctggaac agctcggtcc
     8701 acctcctggg acgggaccac gagtcggtgc ccatcggtgt tcccggagac ctctacatcg
     8761 gtggcgtctg cctggcccgc ggttacctgg gacggccgga cctgaccgcc gaccgcttcg
     8821 tgcccgatcc cttcgccggc gagcccgggg cgcggctcta ccgcaccggg gataaggtcc
     8881 gccggctgcc ggacggcaac ctggactttc tcggccgctt cgatcaccag gtcaaggtac
     8941 gcggcttccg ggtcgagctg gaggaggtcg agtcggtgct gggccgccac cccggagtac
     9001 gccagacggt ggtgctggcc cgcggcgagg gggcggtgga caagagcctg gtggcctacg
     9061 tggtgccgga gcccaccgtc tccggcagcg ccgccggagc ggtcgagtgg cgggccttcc
     9121 tgcgcgagcg tctgccagac tacatggtgc ccggccactt cgtggtcctc gaggcgctgc
     9181 ccctcaacgc caacggcaag gtggaccgcg ccgcgctgcc cgcgcccgcg gcggaccggc
     9241 cggacctcgt ctcccccttc gtagcgcccc gtaacccttc cgaggagttg gtggcggcgg
     9301 tctgggcgga cctcctggga atcgagcgga taggcgtcca cgacgacttc ttcgccctgg
     9361 gaggccactc cctgatggcg acgcgggtgg tgtcgcggct gcgcgacgcc ttccatgtgg
     9421 agctgccgct gcgcgggctc ttcgaggagc cgaccgtggc cgggctcgcc cggcggttgg
     9481 acgaggccgt gcgcgccggc gccggtctcg ccgcaccccc gatcgtgccg gtgccgcgag
     9541 gtgacggaga gatcgagctg ccgctctcct tcgcccagca gcgtctctgg ttcatcgagc
     9601 agctcgagcc cggcagctcg cactacaacg tcgccagccc cctgcggctg aggggcctcc
     9661 tgagagtgga ggctctcgcc ggcgccctga ccggaatcgt gcggcggcac caggtgctgc
     9721 ggacctcctt cccccatacc ggaggacggc cggtgcaggc ggtggctgcg gagtggagga
     9781 cgggcctccc cgttctggat ctgtcctctc tgccggagga ggtgcggcag cgggaggcgt
     9841 cgcacctggc tggagaggag gcgcggcgcc cgttcgatct ggcggcgggg ccgctgctgc
     9901 ggacctctct ggtgcggctc ggcccggcgg agcacgtgct gctcctctgc gtccaccaca
     9961 tcgtatggga tggctggtcc gaggggatct tcgtccgcga gctggccgcg ctctatggca
    10021 ccctggcggc cggccggccg ggcaccctgc cggagctgcc cgtgcagtac gccgacttcg
    10081 cctgttggca gcggcgctgg atgagcggcg aggtgctgga gcggcagctc gcctactggc
    10141 ggcagcggct ggcgggcgct cccgcccacc tcgacctgcc ggcggaccgg cagcgcccgg
    10201 cggcgcccgc cttccgtggt ggcgcgctgc ccttcgcgct gcccgagccg gcttcggccg
    10261 ccgtggccgc cctgggccgg cgccacggtg ccaccctctt catgacgctg gccgcagccg
    10321 tggacgcgct gctgcaccgc tggaccggag ccgaggacgt ggtgatcggc acgccggtcg
    10381 ccggccgcga ccgtctcgag gtggaggggc tcatcggctt cttcgtcaac agcctggtcc
    10441 tgcggaccga tctgtcgggc gacccgaaat tccccctcct tatcgagcgg gcgagggatg
    10501 gcgctctggg agcctatgca caccaggatc tgccgttcga gcgtctggtg gaggagctgg
    10561 ctcccgaccg gagccgcccg cactcaccgc tgttccaggt cctgctggcc ctgcagaatg
    10621 ccccggccgc cgcgctggac ctgccaggga tcgagctgga gccgctggag ctggagcgcc
    10681 ggagcacccg gttcgacctg actctgtcgc tctccgaagg gcgcgaggga atctcgggaa
    10741 cgctggttta cagcgccgat ctgttcgacg cggccacggc cgagcgcatg ctgggccatc
    10801 tgggtgtcct cctggaaggg atcgcggagg aaccggcacg gcggttgtcg gagcttcccg
    10861 tgctgacggc ggccgagcgg cggcagctcc tggaggagtg gaaccagggg agaggggtgg
    10921 cggaggaaga ggggggcctg ttcgacgtgt tcgcggcgcg cgtcgccgag gctccggatg
    10981 gggtggcggt gacgtgtgag gggagggcgc tgagctatgg cgctctgcga gagagggcgg
    11041 aggggctggc ggacgggctg cggcatctgg gagtggggcc ggagtcgcgg gtggggctct
    11101 gtatcgagcg gtcggaggag atgatcgtcg ggatcctggg catcctcgcg gccggtggca
    11161 tgtatgtccc cgtggacccg gagcagccgc aggagcggct cgagttgatc ctccgtgacg
    11221 cgctgtcggg tacggcggct cccgtggtgg tgaccagcgg tgggctggcg gagaaggtgg
    11281 ggagggagat ggcagggcag gggatccgga gggtcggcgt cggggagacc gtcgagggga
    11341 gcgggggagg gccgtgctcg gctccttcgg gtgcgccccc ccttccccct tcccagcgcg
    11401 tccagtcgcc tgcgccggtc ctcccccgct ccccccgacg gccgcgggat gttggggctt
    11461 atgtgatcta tacgtccgga tcgaccgggg tgcccaaggg agtcgtggtg tcgcagggga
    11521 acgtgctgag attgttcgag gcgacggcgg ggcggttccg gttcgggcgt ggagatgtat
    11581 ggacgctgtt tcactcgttt gcgttcgatt tctcggtgtg ggagatctgg ggggctctga
    11641 tgtatggcgg ccggctggtg gtggtgccgc aggaggtggt caggtctcct ggtgagtttc
    11701 accggctgct cgtccgcgag ggggtgacgg tgc
//
LOCUS       DASPJI010000686         4409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_501885, whole genome shotgun sequence.
ACCESSION   DASPJI010000686 DASPJI010000000
VERSION     DASPJI010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501885"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VEL74_19440"
     CDS             complement(<1..610)
                     /locus_tag="VEL74_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64763.1"
                     /translation="MKWTSMSAESLRWVFGLYCAFLGAFVLVTPHHFSTPSYSSLLPY
                     LTLWGAAALGSGVGLLSAAVLQPRRVWSLLIHGLAGATLLALAASFGQRSLWSGFLAY
                     TVLGLGVAAAGGLPYTRPAPDRPAADLFALLMATWAIANGTLMTAVPRLFTAPYFEVV
                     RPYMPTLGPALILSGLLLGWANLRPSPPRKLALTAHLAAGATF"
     gene            660..2945
                     /locus_tag="VEL74_19445"
     CDS             660..2945
                     /locus_tag="VEL74_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HYG64764.1"
                     /translation="MPKRTLRGTPSRLILPVFVLMAVVATGATDSNEQPLLGFSKAGA
                     AEQRALESRFDAALRADNLREWMKRLAARPHPVGSPWGDENARFMLDLFRSWGYDARI
                     EEFQVLFPTPKLRRLEMIAPEPFTAQLAETAVPGDATSGQTADQLPIYNAYSVDGDVT
                     GELVYVNYGVPRDYEDLERRGIDVRGKIVLARYGGSWRGIKPKVAAEHGAIGCLIYSD
                     PRDDGYYQGEVYPEGGWRPEQGAQRGSVADMPLYPGDPLTPGVGATPGAKRLPLKEAP
                     TLTKIPVLPISYGDALPLLRALGGPMAPEGWRGALPVPYRLGPGPAKVRLQLAFDWKT
                     VTAKNVIAVLRGAERPDEWILRGNHHDAWVNGATDPVSGMVAVLEEARAVGELVKSGW
                     RPRRTLVYAGWDAEEPGLLGSVEWAEANAALLRDKAVAYINSDSNGRGFFEVGGSHTL
                     EQFINQVGRDVPDPQTKVSVLERLRARLIVDSRGNELRELRSRADVPIYPLGSGSDYS
                     PFLQHLGIAVLNISYGGEDEYGQYHSAYDSYDHYIRFMDPTFDYGIALAKTGGRAVLR
                     LAQADVLPFEFTRFATTVGRYVDEVESLAGTLREETEERNRRIREGLYELADDPNRKL
                     VVPKPQDPVPHLNFAPLRNAVAAVEKSARAYDQALAAAAQEGRWPAAGERQRLDAILM
                     KMERALTRKEGLPRRPWYVHHVYAPGFYTGYGVKTLPGVREAIEQRQWKEAEEQVAIT
                     ARVLEGFAKEVERAAALLRGE"
     gene            3456..4235
                     /locus_tag="VEL74_19450"
     CDS             3456..4235
                     /locus_tag="VEL74_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64765.1"
                     /translation="MRIRSTVLTLVALCLAALPAIAADRVIQRGVDIWETVPQMTYTS
                     FANNPIPAGFFCAEFPGYTGRMWFKGVPLASDDPGALGTTDTIIERLDNAVFNKNGVA
                     RTRARVRALQLEGIEALKTVCGDYNVKVTLDGEQPVTRMRIVREHAKGGRFFVPLALN
                     VKLTFTRVDNPAEQLEFFEPVRFAVSPHHHWSYRKLAPEAKRIGRVTVDTDGDGTPDT
                     VLPGTSNFRAGNGNKAEFCPQNELGGHAVTEPGPLPTAEAE"
BASE COUNT          710 a         1521 c         1544 g          634 t
ORIGIN      
        1 ggaaggtggc ccccgccgcc aggtgggcgg tgagggcgag cttccgcggc ggcgacggcc
       61 gcaggttggc ccacccgagg agcagcccgc tcaggatcag cgccggcccg agagtgggca
      121 tgtacggccg caccacctcg aagtaggggg ccgtgaagag ccgggggacg gccgtcatga
      181 gcgtgccgtt ggcgatggcc caggtcgcca tgagcagcgc gaagaggtcc gcggccggcc
      241 ggtcgggggc gggccgggtg taggggagcc cgccggcggc ggcgaccccc aggcccagca
      301 cggtgtaggc gaggaagccg ctccacagcg agcgctggcc gaagctcgcc gccagtgcca
      361 ggagggtcgc gccggccagc ccgtggatca ggaggctcca tacccgccgg ggctggagga
      421 ccgccgccga caggagcccc acgccggagc ccagggccgc cgcgccccac agggtcagat
      481 aggggagcag gctggagtac gacggggtcg agaagtgatg cggcgtcacc agcacgaagg
      541 cgccgaggaa ggcgcagtac aggccgaaca cccaccgcag gctctcggcg ctcatgctgg
      601 tccacttcat ctcgattctc cgggctacaa tgcggccact tgccgaagga gcccgtccga
      661 tgccgaaacg tacactccgg ggaaccccat cccggctgat cctgccggtc ttcgtcctga
      721 tggccgtcgt ggccacaggt gcgactgatt ccaacgagca gccgttgctc ggcttcagca
      781 aagccggtgc cgccgagcaa agggcgctcg aatcccggtt cgacgccgcc ctgcgggccg
      841 acaacctgcg ggagtggatg aagcgtctgg ccgcccgccc gcatccggtg ggctccccct
      901 ggggggacga gaacgcccgc ttcatgctgg acctcttccg ttcctggggc tacgacgccc
      961 ggatcgaaga gttccaggtg ctcttcccga cgccgaagct ccggcggctg gagatgatcg
     1021 ccccggagcc cttcaccgcg caactcgccg agaccgccgt gcccggcgac gccacctccg
     1081 gccagaccgc cgaccagctc cccatttaca acgcctactc ggtggatggc gacgtcaccg
     1141 gcgagctggt ctacgtcaac tacggcgtgc cgcgggacta cgaagacctg gagcggcggg
     1201 ggatcgacgt caggggcaag atcgtgctgg cgcgctacgg cggctcgtgg cgggggatca
     1261 agccgaaggt ggcggccgag cacggcgcca tcggctgcct gatctactcc gatccccggg
     1321 acgacggcta ctaccagggg gaagtctacc ccgaaggcgg ctggcgtccg gagcaggggg
     1381 cccagcgcgg ctccgtggcc gacatgcctc tctatccggg cgatccgctg acgccggggg
     1441 tgggcgccac gcccggggcg aagcgcctgc cgctgaagga ggcgccgacc ctcaccaaga
     1501 tccccgtcct ccccatctcg tatggcgacg cgctgccgct gctgcgcgcc ctgggcggcc
     1561 cgatggcgcc ggagggttgg cgcggcgccc tgcccgtccc ctaccggctg ggcccgggcc
     1621 cggcgaaggt ccgcctgcag ctcgccttcg actggaagac ggtcaccgcc aagaacgtca
     1681 tcgccgtgct ccgcggcgcc gagcggccgg acgagtggat cctgcgcggc aaccatcacg
     1741 acgcctgggt caacggcgcg accgacccgg tgagcggcat ggtggcggtg ctggaggagg
     1801 cgcgcgcggt gggcgagctg gtgaagagcg gctggcggcc gcggcgcacc ctcgtctatg
     1861 caggctggga cgccgaagag cccggtctcc tcggctcggt ggagtgggcc gaggcgaacg
     1921 ccgccctgct gcgcgacaag gccgtggcct acatcaactc ggacagcaac ggccgcggct
     1981 tcttcgaggt cggcggctcg cacaccctgg agcagttcat caaccaggtc gggcgggacg
     2041 tccccgatcc gcagacgaag gtgagcgtgc tcgagcggct acgggcgcgg ctcatcgtgg
     2101 acagccgggg caacgagctg cgggagctgc gctcgcgcgc cgacgtcccc atctatcctc
     2161 tgggttcggg ctccgattac agccccttcc tgcagcacct cgggatcgcc gtactgaaca
     2221 tcagctacgg tggcgaggac gagtacggcc agtaccactc cgcctacgac tcgtacgatc
     2281 actacatccg cttcatggac ccgaccttcg actatggaat cgccctggcg aagaccggcg
     2341 gccgcgcagt gctgcgcctc gctcaggcgg acgtcctccc cttcgagttc acccgcttcg
     2401 ccacgaccgt cggccgctat gtggatgagg tcgagagtct ggccggcacc ctgcgggagg
     2461 agacggagga gcgcaaccgg cgcatccggg aagggctcta cgagctggcc gacgacccca
     2521 accgcaagct ggtggtgccc aagccccagg acccggtccc gcatctgaac ttcgccccgc
     2581 tgcgcaacgc cgtcgcggcg gtggagaaga gtgcccgggc ctacgatcag gccctggcgg
     2641 ccgccgcaca ggaaggccgc tggcccgcgg ccggcgagag gcagcggctg gacgccatcc
     2701 tgatgaagat ggagcgggcg ctcacccgca aggagggcct gccgcggcgc ccgtggtatg
     2761 tccatcacgt ctacgccccg ggcttctaca ccggctacgg cgtcaagacc ctcccgggag
     2821 tgagggaggc gatcgagcag cgccagtgga aggaggccga ggagcaggtc gccatcaccg
     2881 ccagggtgct ggagggcttc gccaaggaag tggagcgggc ggcggcgctg ctccgagggg
     2941 agtagggctt ccgtcgagcg gtttgccccg caggtcgagc ggtctgcccc gtggggcaag
     3001 cgactcgccc cgcgggtcaa gaggctcgac ccctggggca ggtggtccgc ccctcgggtc
     3061 aagcgcttcg ccccttgggt cgagaggctt gcccctcggg gcaagaggct tgacccttga
     3121 agcaactggt tcgcccccag ggtcgaagcg tgttgatctc gggtcgagcg gactgccccg
     3181 cgggtcagga ggttcgcccc gcagggcaaa ccaggttgat ctcgggtcga acggtgttga
     3241 tctcggatcc cgcgaggttc accgcgggtc gagcggtctg ccccgtgggg cggagcggct
     3301 cctcctccta gttctcctct ccttcgtggt tctcgttacc ctcttcccgc agagtttccc
     3361 ggacattagc aatctggaag aagcgccatt tctgtcggtg cgctagggtg gatctccggg
     3421 cattgtcgcc cggagagttg aaggaggttg gtgacatgag aatccgctcc accgtcctga
     3481 ccctcgtagc cctctgcttg gccgccctgc cggccatcgc cgccgatcga gtgatccagc
     3541 gcggcgtcga catctgggag accgttcccc agatgacgta caccagcttc gcgaacaatc
     3601 cgattcccgc cggtttcttc tgcgccgagt tccccggcta taccggccgg atgtggttca
     3661 agggcgtgcc tctggcctcc gacgatccag gcgccctggg gaccacggac accatcatcg
     3721 agcgcctcga caacgccgtc ttcaacaaga acggcgtggc gcggacacgg gcccgggtcc
     3781 gggcgctgca gctcgaaggg atcgaggccc tcaagacggt gtgcggcgat tacaacgtca
     3841 aggtgacgct ggacggcgag cagccggtca cgcggatgcg catcgtgcgt gagcacgcca
     3901 agggcggccg cttcttcgtc ccgctggccc tcaacgtcaa gctcaccttc acccgggtgg
     3961 ataacccggc cgagcagctc gagttcttcg agcccgtccg cttcgcggtc agcccgcacc
     4021 accactggag ctaccggaag ctggcgcccg aggccaagcg gatcggccgg gtcaccgtcg
     4081 ataccgacgg cgacggtacg ccggacacgg tgctgcccgg gacctccaac ttcagggcgg
     4141 gcaacgggaa caaagccgag ttctgcccgc agaacgagct tggcggccac gccgtcaccg
     4201 agcccggccc gctgcccacc gccgaggccg agtagcaggt gatccctttc aatcaaacgg
     4261 gaggtggtca cgatttgtga ccaccttcct tctttaatcc accggaaacc accgcgcgcg
     4321 ggccaggaag ccgagccgcg agaagaggcc gagctggggc ggggtctgga ggaggctgat
     4381 gtcaccatcc tccagatcct cgatcgcgg
//
LOCUS       DASPJI010000687         7222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_557507, whole genome shotgun sequence.
ACCESSION   DASPJI010000687 DASPJI010000000
VERSION     DASPJI010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7222)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557507"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VEL74_19455"
     CDS             <1..920
                     /locus_tag="VEL74_19455"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HYG64766.1"
                     /translation="EALDRKAREFDPVVKIGRTHLQDAVPVRLGQEFGGYAQQVRNSL
                     ARLEAVKPRLAELPLGGTAVGTGLNAHPEFAGRVIRRLAERTGCPFVPAPNRFEAMAA
                     KDAAVETSGALKTIATSLTKIANDIRWLASGPRCGIGEISIPSLQPGSSIMPGKVNPV
                     IPESVLMVAAQVVGNDATITLAGMSGNFDLNVMMPLIAYNLLQSIEILANASVLLAAR
                     CVEGVEANRERCEELVERSLAMVTSLVPRIGYDAAAEIAKESVRTGRTVRELCRERQV
                     LPEAELNEALDPWAQTEGGIHAGGAGAGG"
     gene            complement(932..1306)
                     /locus_tag="VEL74_19460"
     CDS             complement(932..1306)
                     /locus_tag="VEL74_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64767.1"
                     /translation="MAEPIQVPDQPPLRRPGAARPRSVPHTHMGGPRREPAAPPRAPE
                     PSITDPESDAQASRCRGRQILLAGLILALGLNVAALAVSLSARGGALGGAEILLAAAT
                     LVSGSVLLLLVEMGRRRDRRRS"
     gene            complement(1418..1693)
                     /locus_tag="VEL74_19465"
     CDS             complement(1418..1693)
                     /locus_tag="VEL74_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64768.1"
                     /translation="MDVSNTTGSDSDYKVTGGGGTRGPGGWIKLPGHRKVRHEVEHDE
                     GPWKIEFKVAANGSEFKIFGRCNDPDAHVVLVERGGAYGVDVQTNGH"
     gene            complement(1784..2734)
                     /locus_tag="VEL74_19470"
     CDS             complement(1784..2734)
                     /locus_tag="VEL74_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64769.1"
                     /translation="MRHTFEGDDDLLRRYLLGELSEEETARLEPRLLEDDRLLEQMEA
                     VEGDLLAEYAQGELTEEQEREVGRRLTATEEGRARLALARSLHRIANETPAEQKPRAT
                     VVPMPRVSSRRPSWYAPMAAMAAALLLGVVGLSVWPQLQGRFGEHPPAEVSNNNIGEA
                     IIRTPAPDPVSPVEATPGEQPAEPAAETPVSTEIVTAPEPKPEPVGVELPLRSGQRSG
                     EAQDVLRREVPGNAEKIEFRVPLRDEGFTSYKIVLYGPDRPEDVFPGLELRPLASGRG
                     LRFQVPAAQVSDGLYRIEAFGVTASGDDELIGDQEFEIQR"
     gene            complement(2810..3352)
                     /locus_tag="VEL74_19475"
     CDS             complement(2810..3352)
                     /locus_tag="VEL74_19475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64770.1"
                     /translation="MSQEAFEALLRELDPDDRDRAAELYLQLRRKLIRVFEWRGCSSP
                     EELVDETLDRVGQRLAEDVKPNNPASYAQGVARHIYQEAVRREAMEQKRLKSGDWPPH
                     SPEDPEPDDRRLEALRDCLEKLGQDQRRLVLVYHQSDSHIEHRKKLSNELGIPINALR
                     IRVHRLRRRLAECIEARLGN"
     gene            complement(3379..6660)
                     /locus_tag="VEL74_19480"
     CDS             complement(3379..6660)
                     /locus_tag="VEL74_19480"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HYG64771.1"
                     /translation="MSRLLLPSSTPGFGVARGALCWALAALLAQACAAPRGGPSSPGA
                     ERAAAVTEHEIGPGESRPLVIELRAGQFVRAVVEQAGADVEVELLDPSAESVQKVDSP
                     YGRNGLEDLAAVARRPGSHQIVVRSGERSDTGRYRIRIEGPRAPSEKDRHRAEAVSLT
                     QRAVNLMGGRSPEAALREQVRLREQALPLWIQAGDRQREADTLFQLGTAHLALRENEE
                     AVRYLHRASQLFEELDATVDLARTLNQAGRVDSALDRFQHAREHWEKALPLARRSGDR
                     RLLFDISSNLGTFHINELGEPRTAIEVYLEEALGLAESVDRLAQTKAHINLGSAYADL
                     SEYEIALEHSGRALQLARTLAGPDREVEEVTALNNLGDTYFALGELEKASQHFRQVLE
                     HEGVEPLKRAKTLQNLGVALDMSGKTDEALRAFEGALDLARLIEDKDLQAYILLNRGN
                     LHLGEREYQRALEAGRQALTLASRPETRARTLHILGASHRNLENLPAAREALSQALDL
                     SQADLALRADITMVLAKVEKDAGELEKAGELAGQAIEAIEQLRDRVVRQDLRETFLAS
                     KQGFYEFYVDILMELERRNPGRGFAGRALDMNERARARGLLDLLAEVEADLSRDADAG
                     LLAQERALRAEINALYQHRLKLIKDGVTGQRLEQVGDRLDEAIEKLYRVTADLKASSP
                     HYAALARPEPLTSAQIQEKVLDRDSALLEYALGEDRSWLWVVRPDSIHTFELPKRAQV
                     EKEAREYHGQVSLQPPDEEAGRNKADRAARRAGEALSRMLLEKAEPLLRGRRLLVVSD
                     GALHYVPFGALPLPSLRKGSPSPLLDHNEVVYLPSASTLAVLREDEAKRRRPDKVVAA
                     FGDPVFQERDDRLRRLRGDLATQSRAAGSEHRGFGSRADSFDPSTLPRLRFTQEEVNA
                     IVEFADPERSFKALGFDATLAEVTSGRLAEYRIVHFATHGHIATDKPELSGLVFSRFD
                     GRAEPLEGVLRLTDVYSLRLNADLVTLSACETALGQEMRGEGLIGLTRGFMYAGSSRV
                     LATLWKVDDRTTMKLMKRFYEAYLREGQPAPAALRQAQREIRKDPWRRHPYYWAGFSL
                     QGESR"
     gene            6840..>7222
                     /locus_tag="VEL74_19485"
     CDS             6840..>7222
                     /locus_tag="VEL74_19485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762117.1"
                     /note="involved in translesion DNA polymerization with
                     beta clamp of polymerase III; belongs to Y family of
                     polymerases; does not contain proofreading function;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HYG64772.1"
                     /translation="MTEPQLPAGPPVGDDPAPRRVLHCDMDCFYAAVHMRDDPRLRGK
                     PVVIGGRPEGRGVVAAASYESRRFGIHSAMPSSRALRLCPEVIFIPPDFRKYSRESEK
                     IFAIYRELTPLVQAVSIDEAYLDVT"
BASE COUNT         1009 a         2526 c         2526 g         1161 t
ORIGIN      
        1 acgaggcgct ggaccgcaag gcgcgggagt tcgacccggt ggtgaagatc ggccgcaccc
       61 acctgcagga cgcggtgccg gtccgtctcg gccaggagtt cggcggctac gcgcagcagg
      121 tccggaactc gctggcgcgg ctcgaagcgg tcaagccgcg gctggccgag ctccccctgg
      181 gcgggacggc cgtgggcacg ggcctgaacg cccaccccga gttcgccggc cgggtgatcc
      241 ggcgcttggc ggagcgcacc ggctgcccgt tcgtgccggc gcccaaccgc ttcgaggcga
      301 tggcggccaa ggacgccgcg gtcgagacct cgggagcgct caagaccatc gccacgtcgc
      361 tgaccaagat cgccaacgac atccgctggc tcgcctccgg gccgcgctgc ggcatcggcg
      421 agatctccat ccccagcctg cagccgggga gctcgatcat gccgggcaag gtcaacccgg
      481 tcatcccgga gtcggtgctg atggtggccg cgcaggtggt gggcaacgac gccaccatca
      541 ccctggccgg catgtcgggc aacttcgacc tcaacgtcat gatgccgctc atcgcctaca
      601 acctcctgca gtcgatcgag atcctggcca acgcctcggt gctcctggcg gcccggtgcg
      661 tcgagggggt cgaggccaac cgcgagcgct gcgaggaact ggtggagcgg tcgctcgcca
      721 tggtgacgtc gctggtgccg cggatcggct acgacgccgc cgccgagatc gccaaggagt
      781 cggtgcgcac cggccgcacg gtgcgcgagc tgtgccgcga gcggcaggtg ctgcccgagg
      841 ccgagctcaa cgaggcgctc gacccctggg cgcagaccga ggggggcatc cacgccggcg
      901 gcgccggcgc gggcggctag ggctcgggaa gctaggagcg gcggcggtcc cgccgccgcc
      961 ccatctccac caacagaagc aggacggagc cgctgaccaa cgtggcggcg gcgagcagga
     1021 tctcggcgcc gccgagcgct ccgccgcggg ccgacagcga gaccgccagc gcggcgacgt
     1081 tgaggcccag ggccaggatc aggccggcga ggaggatctg gcgcccccgg cagcgggacg
     1141 cctgggcatc gctctcgggg tcggtgatgg agggttcggg agcccgcggc ggggcggccg
     1201 gctcccgccg cggacctccc atgtgggtgt gggggacaga ccgtggccgg gccgctccgg
     1261 gacgccggag gggaggttga tctggcacct ggatcggctc agccacgttc gcgaccactc
     1321 ctgccttgag tgacggacgg ggacgaaacg tttcacagac cccaaaaaac aggggcggag
     1381 ccgtcgccgg ccccgccccg ggattcttca acgctgctca gtgaccgttg gtctggacgt
     1441 cgactccgta ggccccgccc ctttcgacga ggaccacgtg agcatcgggg tcgttgcacc
     1501 ggccgaagat cttgaactcg ctgccgttgg cggccacctt gaactcgatc ttccagggcc
     1561 cctcatcgtg ctccacttcg tgtcggacct tccggtgccc cggcagcttg atccagccgc
     1621 ccggaccgcg ggtaccgccg ccgcctgtga ccttgtagtc ggaatctgaa ccggtcgtgt
     1681 tggatacatc catcgcaggg aacctccatg gtacagcccg ctcgacgctc ggtacgaggg
     1741 gcgggttgaa cggcaccaac gtgaagctgc gtgaacgcgc ccgttacctc tggatctcga
     1801 attcctgatc gcctatcagc tcgtcgtcac cgctcgccgt gacgccgaag gcttcgatcc
     1861 tgtacaaccc atcagacacc tgcgccgccg gcacctgaaa ccggagcccc cggccagagg
     1921 ccagcggccg cagctcgagg cccgggaaaa cgtcctcggg gcgatctggc ccgtagagca
     1981 cgatcttgta agaggtgaac ccttcgtccc ggagagggac gcggaactcg atcttctcgg
     2041 cgttcccggg gacctcacgc cgcaggacgt cctgcgcctc gccgctacgc tgccccgagc
     2101 ggagcggcag ctcgacgcct accggctccg gcttgggctc cggggccgtc acgatctccg
     2161 tagagaccgg tgtctcggcg gcgggctccg cgggctgctc tcctggtgtg gcctccacgg
     2221 gggatacggg atccggcgcg ggagtgcgga tgatggcctc accgatgttg ttgttgctga
     2281 cctctgccgg aggatgctcc ccgaaccggc cctggagctg gggccagacc gacaggccca
     2341 cgacccctag cagcagagcc gccgccatgg ccgccatcgg ggcgtaccag gaggggcgcc
     2401 tggatgagac gcgcggcatc gggaccaccg tggctctggg tttctgttcg gcgggcgtct
     2461 cgttggcgat gcgatggagg ctccgcgcca gcgccaggcg ggcccggccc tcctccgttg
     2521 cggtcaggcg cctcccgacc tcccgttcct gctcctcggt gagctccccc tgggcgtact
     2581 cggccaggag gtcaccctcc accgcctcca tctgttccag cagacggtcg tcctcgagaa
     2641 gccgcggttc gaggcgcgcc gtctcctcct cactcagctc ccccagcagg taccgccgca
     2701 ggaggtcgtc atcgccttcg aacgtgtgtc gcattccgac cggctccttt cctcatccag
     2761 ccctgagtgt cagaaagggg aaaagcgttt cgcgggggtg atcgggggat cagttgccga
     2821 ggcgcgcttc gatgcactcg gcgagcctgc ggcgcagccg gtggacccgg atccgcaggg
     2881 cgttgatggg gatccctagc tcgttcgaga gcttcttgcg gtgctcgatg tggctgtcgc
     2941 tctggtgata gaccagtacc aggcgccgct ggtcctgtcc cagcttctcg aggcagtcgc
     3001 ggagagcctc gagccggcgg tcgtcgggct ccggatcctc cggcgagtgg ggcggccagt
     3061 cgccgctctt cagcctcttc tgttccatcg cctcgcgccg gaccgcctcc tggtagatgt
     3121 gacgggcgac accctgggca tagctggccg ggttgttcgg cttcacatcc tcggcgaggc
     3181 gctggcccac ccgatcgagg gtctcgtcga ccagctcttc gggagaggag cagccgcgcc
     3241 actcgaagac gcggatcagc ttgcggcgga gctgcagata gagctcggcc gcccggtcgc
     3301 ggtcatcagg gtccagctcc cggaggaggg cctcgaaagc ctcctggctc atcccttcct
     3361 cctctcgggg cttgcgtttc accggctctc accctggagg gaaaagcccg cccagtaata
     3421 ggggtgccgg cgccatgggt ccttccggat ctcgcgctgc gcctgccgca acgccgccgg
     3481 tgcgggctgg ccttcgcgga ggtaggcttc atagaagcgc ttcatcagct tcatcgtggt
     3541 gcggtcgtcg accttccaga gggtggcgag cacccgcgac gaccccgcgt acatgaagcc
     3601 ccgcgtcaag ccgatcagcc cttcgccgcg catttcctga cccagagcgg tctcacaagc
     3661 ggagagggtg accaggtcgg cgttgagccg caggctgtag acgtcggtca ggcggagaac
     3721 gccctccagg ggctcggccc ggccgtcgaa cctggagaag accagacccg acagctccgg
     3781 cttgtcggtg gcgatgtgcc catgggtggc gaagtgcacg atccggtact cggcgagccg
     3841 cccgctggtc acttcggcca gtgtagcgtc gaaccccagg gccttgaagc tccgctccgg
     3901 gtccgcgaac tcgacgatgg cgttgacctc ctcctgggtg aagcggaggc gcgggagggt
     3961 ggaggggtcg aacgaatcgg ccctcgaccc gaagccccgg tgctcggagc cggcggcgcg
     4021 gctctgggtc gccaggtcgc cccggagccg gcgcagacga tcgtcgcgct cctggaagac
     4081 ggggtcgccg aacgcggcca cgaccttgtc gggcctgcgg cgcttcgcct cgtcctcccg
     4141 cagcacggcg agggtggagg cggaggggag gtacaccacc tcgttgtggt cgaggagcgg
     4201 agaggggctg cccttccgga gcgaagggag gggcagggcg ccgaagggga cataatggag
     4261 ggcgccgtcg ctgaccacca ggagacggcg ccccctgagg agcggctcgg ccttctccag
     4321 gagcatccga gagagagcct ccccggctcg ccgcgccgcc cgatcggcct tgttcctgcc
     4381 ggcctcctcg tccggcggct ggagcgagac ctggccgtgg tactcccgag cctccttctc
     4441 gacctgggcc ctcttgggca gctcgaacgt atggatggag tccggccgga cgacccagag
     4501 ccagctccgg tcctcgccca gcgcgtactc gagcagcgcg gagtcgcggt ccagcacctt
     4561 ctcctggatc tgtgccgagg tcagcggctc cggccgggcg agcgccgcgt agtgggggct
     4621 gctggccttg aggtccgccg tcacccggta gagcttctcg atggcctcgt cgagacggtc
     4681 ccctacctgc tccagccgct ggccggtcac cccgtccttg atcagcttca gccggtgctg
     4741 atagagggcg ttgatctcgg cgcgcaacgc ccgttcctgc gccagcagcc cggcgtcggc
     4801 gtcccggctc aggtcggctt cgacctccgc caggaggtcg agcaggcccc gcgcccgcgc
     4861 ccgctcgttc atgtccagcg cccggcccgc gaagccccgg ccggggttcc ggcgctccag
     4921 ctccatcagg atgtcgacgt agaattcgta gaacccctgc ttggaggcga ggaacgtctc
     4981 ccgcaggtcc tgcctcacca cccggtcgcg gagctgctcg atggcctcga tcgcctggcc
     5041 ggcgagctcg ccggccttct cgagctcgcc cgcgtccttc tcgaccttgg cgagcaccat
     5101 ggtgatgtcg gccctcaagg cgaggtcggc ttggctgagg tccagcgcct ggctcagggc
     5161 ttcgcgcgcg gccggcaggt tctccaggtt ccggtgggag gctcccagga tgtgcagggt
     5221 ccgcgcccgg gtctccgggc gggatgcgag ggtgagggcc tgcctgcctg cttcgagggc
     5281 gcgctggtac tcgcgttcgc cgagatggag attgccgcgg ttcagcagga tataggcctg
     5341 gaggtccttg tcctcgatca ggcgcgcgag gtccagcgcc ccctcgaagg cgcggagcgc
     5401 ctcgtcggtc ttgccggaca tgtccagggc gaccccgagg ttctggagcg tcttggcgcg
     5461 cttcaggggc tcgacgccct cgtgctcgag gacctgccgg aagtgctggc tcgccttttc
     5521 cagctcaccc agggcgaagt aggtgtcgcc gaggttgttg agcgcagtga cctcctcgac
     5581 ctcccgatcc ggaccggcga gggtgcgagc cagttgcaag gcccggccgg agtgctccag
     5641 ggctatctcg tactccgaca ggtcggcgta ggccgagccc aggttgatat gggccttggt
     5701 ctgcgccagg cggtccacgg actccgcgag gccgagcgct tcctctaaat agacctcgat
     5761 ggcggtccgt ggctctccga gctcgttgat gtgaaacgtg ccgaggttgc tcgagatatc
     5821 gaacagcagg cgccggtccc cggagcggcg ggccaggggc agtgccttct cccagtgctc
     5881 ccgcgcgtgc tggaaccgat cgagagccga gtcgacgcgc cccgcctggt tgagggtgcg
     5941 ggccaggtcg accgtcgcgt ccagctcctc gaagagctgc gaagcgcggt ggaggtaccg
     6001 gaccgcctcc tcgttctcgc gcaaagctaa gtgcgcggtc ccgagctgga acagcgtgtc
     6061 ggcttcccgc tgccggtccc cggcctggat ccagagtggc agtgcctgct cccgcaaccg
     6121 gacctgctcg cggagggccg cctcggggct cctgcccccc atgagattca ccgcccgctg
     6181 ggtgaggctc accgcctccg cccggtgccg gtccttctcg ctcggtgccc gcggcccttc
     6241 gatccggatc cggtagcggc cggtgtcgct tctttcccct gagcgcacca cgatctggtg
     6301 cgagcccggc cgccgggcca cggctgcgag gtcttcgagc ccgttccggc cataggggct
     6361 gtccaccttc tggaccgact cggcgctggg gtcgagcagc tcgacctcga cgtcggcgcc
     6421 ggcctgctcg acgacggcgc gcacgaactg gcccgccctg agctcgatca cgagaggccg
     6481 gctctcgccc ggcccgatct cgtgctcggt gaccgcggcc gcccgctcgg caccagggga
     6541 ggaggggccg ccgcgggggg ccgcgcacgc ctgggccagg agggccgcga gggcccagca
     6601 cagggctccc cgggccaccc cgaaacccgg cgtgctggag ggaagaagaa ggcggctcat
     6661 cctgatttcg gttcggctga cttcacgacc tgcgcccccg aaccgatggc aggcccggtg
     6721 ggtggagctg tgtccaaatc accaatattg tacgcgtttc cgatggtgtt catggaatgg
     6781 ggggctcctg ccccttgggg ctcgcgcccg agttgcgcta agctttccct gtggaaaccg
     6841 tgacggagcc gcagctcccg gcggggcctc cggtgggcga cgacccggcg ccccggcgtg
     6901 tcctgcactg cgacatggac tgcttctatg ccgccgtcca catgcgggac gatccgagac
     6961 tgcgcggcaa gccggtggtc atcggcggcc ggcccgaggg acgtggcgtc gtcgcggcgg
     7021 cgagctatga gtcccggcgc ttcggcatcc attccgcgat gccgtcgtcc cgtgccctgc
     7081 gcctctgccc ggaggtgatc ttcatccccc cggacttccg caagtactcg cgcgagtcgg
     7141 agaagatctt cgccatctac cgcgagctca cccctctggt ccaggcggtg tcgatcgacg
     7201 aggcctacct cgacgtcacc ga
//
LOCUS       DASPJI010000688         2441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_611787, whole genome shotgun sequence.
ACCESSION   DASPJI010000688 DASPJI010000000
VERSION     DASPJI010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611787"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(488..1108)
                     /gene="purN"
                     /locus_tag="VEL74_19490"
     CDS             complement(488..1108)
                     /gene="purN"
                     /locus_tag="VEL74_19490"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HYG64773.1"
                     /translation="MPARLGILLSGRGSNFLSLHAAIERGGLPAEIALVVSNIPGAGG
                     LARARELGLPALALPHQGIPRDAHEAQVLAALRSAEVDWVCLAGYMRVLSPRLVTAFP
                     RRIVNIHPSLLPAFPGLHAQEQALEWGVRVAGCTVHLVDEGLDSGPIVVQRTVPVEDG
                     DTAATLAARILEQEHEAYPEALRRLLTEPWEVRGRRLIFRKVGEGG"
     gene            complement(1123..2220)
                     /gene="purM"
                     /locus_tag="VEL74_19495"
     CDS             complement(1123..2220)
                     /gene="purM"
                     /locus_tag="VEL74_19495"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HYG64774.1"
                     /translation="MSGSRPSAYAQAGVDIDAQEKGLARVKKLARSTFTPGVLTEIGS
                     FGGLFRPDLAGMTEPVLVASADGVGTKLVVARMAGDYSSVGRDLVNHCVNDILVQGAE
                     PLFFMDYLGAGVLEPEKMVELVSGVAQACRDNGCALLGGETAEMPGFYQPGDYELVGF
                     IVGLVDRPKVLDGSRVRPGDVLVGLPSSGLHTNGYSLARRILFDKLGLAVADRAPWQD
                     KKETVGEALLATHLSYLEPLRPLLGHPDLHAMAHITGGGLTDNLPRVLPAGSRAEVRA
                     GSWPVPELFHFLQERGEVDAEEMFRVFNMGIGMVLVVSPSGLREVLGLLRSKGQRSWL
                     IGTVQEGSPAGGASVSYDLGREDGAPVLPGA"
BASE COUNT          362 a          862 c          860 g          357 t
ORIGIN      
        1 gcacggatcc ggctaaagac cgtacgcgct tgttatctct cggaatcgcg cagactcgag
       61 cagacaggac caaagcccca cccgcgagcc gcgctggtcg ccgacttcac tcttcctcta
      121 ttcagttttc aaagaaccgg ctctgttggc cgcgcccccg ttcggggcga cttttgattc
      181 tacggccgct cggcggggct gtcaacccct cttcgcgtcc ggcccgactt tcaacccagc
      241 gcgaccgcgc gttattccgg gcctccgaag aagccccgag attcgccacg ttgtcagggg
      301 agcggctccg aagcctcttg gcccctcgga gcgctgggag tcgcagactc tacaacagac
      361 cgagcccgcc gtcaattccg ccgttttcct tcgggggtcc ctcgcgagct cccgtccggg
      421 tcttcaaaga atcgccgagg tcattccggc aacaggaggc gcagtctagg gaaccctggc
      481 ggggctgtca acccccttcg ccaactttcc tgaagatcag ccgccggccc cgcacctccc
      541 agggctcggt cagcagccgg cgcagggcct ccggataggc ctcgtgctcc tgctcgagga
      601 tgcgggcggc gagggtggcg gcggtgtcgc cgtcctcgac cggcacggtg cgctgcacca
      661 cgatcgggcc gctgtcgagc ccctcgtcga ccaggtggac ggtgcagccc gcgacccgca
      721 ccccccactc cagcgcctgc tcctgggcgt ggagccccgg gaaggccggc agcaggctgg
      781 ggtggatgtt gacgatgcgc cgcgggaagg ccgtcacgag ccgcggcgac agcacccgca
      841 tgtagccggc gaggcagacc cagtccactt cggcgctgcg cagggcggcc agcacctgcg
      901 cctcgtgggc atcgcggggg atcccctggt gcggcagggc gagcgccggc agccccagct
      961 cgcgggcgcg ggccaggccg cccgccccgg ggatgttgct gaccaccagg gcgatctcgg
     1021 ccggcagccc gccgcgctcg atggcggcgt ggagggacag gaagttgctg ccgcggccgg
     1081 agagcaggat gccgaggcgg gcgggcatga tcgtcaggaa agtcaggccc cggggaggac
     1141 gggcgcgccg tcctcgcgcc ccaggtcgta ggagacgcta gcccctcctg ctgggcttcc
     1201 ttcctgaacg gtgccgatga gccacgaccg ctgccccttg ctccgcagca gcccgagcac
     1261 ctcgcgcagc cccgaggggg acaccaccag caccatgccg atccccatgt tgaagacccg
     1321 gaacatctcc tcggcgtcca cctccccccg ctcctgcagg aagtggaaca gctcgggcac
     1381 cggccagctc ccggcgcgga cctcggcgcg gctgccggcg gggaggaccc gcggcaggtt
     1441 gtcggtcagc ccgccgccgg tgatgtgggc catggcgtgg aggtccgggt ggccgaggag
     1501 cggccgcagc ggctcgaggt aggagagatg ggtggcgagg agcgcctcgc ccaccgtctc
     1561 cttcttgtcc tgccaggggg cgcggtcggc gacggccagg cccagcttgt cgaagaggat
     1621 gcggcgggcc agggagtagc cgttggtgtg caggcccgag gaggggaggc cgacgaggac
     1681 gtcccccggc cggaccctgg agccgtccag caccttgggg cggtccacca gcccgacgat
     1741 gaagccgacc agctcgtagt cccccggctg atagaagccg ggcatctcgg cggtctcgcc
     1801 gccgagcagg gcgcagccgt tgtcgcggca ggcctgggcg acgcccgaca ccagctcgac
     1861 catcttctcc ggctcgagga cgccggcgcc gaggtagtcc atgaagaaca gcggctcggc
     1921 cccctgcacc aggatgtcgt tgacgcagtg gttgaccagg tcgcggccga ccgaggagta
     1981 gtcgccggcc atgcgggcga ccaccagctt ggtgccgacg ccgtcggccg aggcgaccag
     2041 gacgggctcg gtcatgccgg cgaggtccgg ccggaagagg ccgccgaagc tgccgatctc
     2101 ggtgaggacg cccggggtga aggtggaccg cgccagcttc ttgacccggg cgagcccctt
     2161 ctcctgggcg tcgatgtcga cgccggcctg ggcgtacgcg ctgggtctgg atccgctcat
     2221 ggctgggccg ctcgcgccgg aactataccc agtgtcgaag cgggtgtcga tctgtgtcga
     2281 agtgtgtcgg cggatgtcgg acacaggggt cccgccccct tctccgagga gaggcggggt
     2341 ggcaccgaac ctgctctgtc caggggtgca gcacaatcaa ctcttccccc tccctgccgg
     2401 aaaaggagat tcgccatggt cgccgatgtc tcttcccccg c
//
LOCUS       DASPJI010000689         5140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_849714, whole genome shotgun sequence.
ACCESSION   DASPJI010000689 DASPJI010000000
VERSION     DASPJI010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849714"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..1993
                     /locus_tag="VEL74_19500"
     CDS             311..1993
                     /locus_tag="VEL74_19500"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HYG64775.1"
                     /translation="MSAWALDLGTTNSGVAFWDDSLGKPRLVELPDVCRKIDANDPLE
                     APRLVPSAVHFLEKPGTLDRLGSWPPLARRFFIGRRALIGRQALERNDGVVHPSFVPT
                     FKPGLSGEALRPLARVGRESLTARDAAFAFLRELLREVKRATGQRIRDMVVTSPVSAF
                     EMYRAEAQGLLRQLGVGRVRFVDEPVAAALGYGISLSHPRTVLVVDVGGGTMHVVLVR
                     LTPQGAVSGQATVLAKRGRPLGGNAVDGWVLQDLCNRMGHDLSRDGDDEVTRLWRRMM
                     LAEACRVKEAVFFREEEAFLVSPGDLPARHAPRPRGDGLVTLTRAGLGEILEKNGFFR
                     SLAASLEEVLEQAGVKPDDVEDVLLVGGSTLLPGFFSFFEERFERRRVRAWQPFEAVA
                     YGAACFAGNRMDALDFIVHDYAFVTHDPKTGDPQHTVIVPRGTRFPTPTELWKRQLVP
                     TCALGEPETLFKLLICEVARGDGDDRRFVWDAAGDLHKVGGAAGDAQVVVPLNEASPT
                     LGYLDPPHSPRDHRPRLEIAFGVNAERWLIATVRDLLTQRELMREEPVIRLV"
     gene            2006..3313
                     /locus_tag="VEL74_19505"
     CDS             2006..3313
                     /locus_tag="VEL74_19505"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HYG64776.1"
                     /translation="MEPFITFGLPLLAGGIYLLGILARHRQLLDQWQALAAKHELAVL
                     ETSSVAASRVSVAARAGVMEVRFEKIPKRGLWVHLTIPGLLATSGLRLRREPFVALGA
                     REIETGDESFDQTFLVEGSAGLVMALLDSKARRLLLQINVEGELEIRGGVVSVRVTDT
                     TLPQLLPLLLELGQRFTTGSDTVRRLAASAREDREAGVRLRSLLLLEREFPREQVTRD
                     TLRAACSDPAPQIRLRAAIARGAEGRETLMALAEDPEVDDDWSAQAVSTLGRQLPHER
                     TQEILRQSLRRRRLGTAHACLAALGQWRGAALDELVKVLEREPDALAAAAALALGESG
                     EAAAEPPLLRALQHGSAEIRSAAATALGRVGTAAAVLPLKEAAEEDRELRRVARQSVA
                     EIQSRIEGASPGQLSLAQSEAGQLSLATDPSGQLSLRSERPVA"
     gene            3461..4654
                     /locus_tag="VEL74_19510"
     CDS             3461..4654
                     /locus_tag="VEL74_19510"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HYG64777.1"
                     /translation="MTKTKLHLLVRADRIEVRFEHASYQLTRVIVELPALPWFSGVRI
                     RGDDWKPFGAREIEVGDWVFDTRFFVEGPERLVVRMLDAEMRRLLLEAGSKGDLEIRE
                     GTLQVVALDHRLPEVLPILLELAQRFMRQSTNAAPGLAANARRDPEPGVRLRNLIHLA
                     RDFRDEQLTHETLRAACRDPSPQVRLQAGTALGAEGRDVLRELAEDAEGTIEDAISSL
                     AVAALGAELPLERVQAILRRSLRDRRIQTARVCLERLGRSGDTTAVPTLAKILALEKD
                     ELAIAAAVALGITGDEAAEPPLIQALDRDDNGLGVAAANALAHIGSAASVLPLKEAAR
                     SGDQALRQAARQAVAEIQSRFHPGASPGQLSLAQAEAGQLSLATDPAGQLSLPPQEPE
                     KGPGG"
     gene            4682..>5140
                     /locus_tag="VEL74_19515"
     CDS             4682..>5140
                     /locus_tag="VEL74_19515"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYG64778.1"
                     /translation="MSDGTPSPPRRRALGTVFLTILLDLIGFGMILPLLPFYAQELDA
                     TPFQIGLLFASYSLAQLAFAPLLGRVSDRAGRRPVLLASILGSCGAYVLFWAAGSYAL
                     LLVARTLSGVAASNYGIAQAYVADVTPPEGRSKAMGLVGAAFGLGFIIGPA"
BASE COUNT          684 a         1895 c         1828 g          733 t
ORIGIN      
        1 cgccggcgtc cctcagcact gaggaacagc tccttagtgc ttcgccgccg tccctcaatg
       61 gtgaggaacg actcctcacc agttccctgt cgcagggaag cgttgaggag cggcgcctca
      121 ctgttgagga acggcaggcc ccgaggagcg ggacccgcct ccgcagcgga cggcaagagg
      181 aaagtggaag aatcttgcgg ggggagcttt gggttgcggg ggcggaggaa aaaccacagg
      241 ctgtgctgaa accgggagag aaggccgagg ggtgaggggc taaggccgcc atctatataa
      301 tggatcccca atgtccgcct gggctctcga cctcgggacc accaattccg gagtggcgtt
      361 ctgggacgac tccctgggga agcctcgtct cgtcgagctg cccgacgtct gccggaagat
      421 cgacgccaac gacccgctgg aggcgccgcg gctggtgccg tcggccgtcc acttcctcga
      481 gaagcccggg acgctcgacc gcctggggag ctggccgccg ctggcgcggc gcttcttcat
      541 cggccggcgg gcgctgatcg gccgccaggc gctggaacgc aacgacggcg tggtgcatcc
      601 gtcgttcgtc cccaccttca agcccggcct gagcggcgag gcgctgcggc cgctggcccg
      661 ggtgggtcgc gagagcctga ccgcccgcga cgccgccttc gccttcctgc gcgagctgct
      721 gcgagaggtc aagcgggcga ccgggcagcg catacgcgac atggtcgtca cctcgccggt
      781 gtcggcgttc gagatgtacc gcgccgaggc gcaggggctg ctgcgccagt tgggagtcgg
      841 ccgggtccgc ttcgtcgacg agccggtggc ggcggcgctc ggctacggaa tcagcctcag
      901 ccacccgcgg accgtgctcg tcgtcgacgt cggaggcggc accatgcacg tcgtcctcgt
      961 ccgcctcacc ccgcaggggg cggtcagcgg ccaggccacg gtgctcgcca agcgcgggcg
     1021 ccccctgggg ggcaacgccg tcgacggctg ggtgctgcaa gacctctgca accgcatggg
     1081 ccacgacctg agccgcgacg gcgacgacga ggtcacccgc ctctggcggc ggatgatgct
     1141 cgccgaggcc tgccgggtga aggaggccgt cttcttccgc gaggaggagg ccttcctggt
     1201 gagcccgggg gacctgccgg cgcgccacgc gccccgtccg cgcggagacg gcttggtgac
     1261 gctgacccgc gccggcctcg gggagatcct cgagaagaac ggcttcttcc gcagcctggc
     1321 cgcgtcgctg gaggaggtat tggagcaggc cggggtcaag cccgacgacg tcgaggacgt
     1381 gctgctggtg gggggctcga cgctcctccc cggcttcttc tcgttcttcg aggagcgctt
     1441 cgagcgccgg cgggtacgcg catggcagcc gttcgaggcg gtggcctacg gcgccgcctg
     1501 cttcgccggc aaccggatgg acgccctgga cttcatcgtc cacgactacg ccttcgtcac
     1561 ccacgatccg aagaccggcg atccccagca caccgtcatc gtgccgcggg ggacgcgctt
     1621 ccccaccccg accgagctgt ggaagcggca actggtcccc acctgcgcgc tcggcgagcc
     1681 ggagaccctg ttcaagctcc tcatctgcga ggtggcgcgc ggcgacggcg acgaccggcg
     1741 cttcgtctgg gacgccgccg gcgacctcca caaggtgggg ggcgcggccg gcgacgcgca
     1801 ggtcgtcgtg ccgctgaacg aggcgagccc cacgctcggc tatctcgacc cgccccactc
     1861 cccccgcgac caccggccgc ggctcgagat cgcgttcggc gtcaacgccg agcgctggct
     1921 gatcgccacc gtccgcgacc tcctcacgca acgggagctg atgcgcgagg agccggtcat
     1981 ccggctggtc tgaggaggcg ccggcatgga gccgttcatc accttcggcc tccccctgct
     2041 cgccggcggc atctatctcc tcggcatcct ggcccgccac cgccagctcc tggatcagtg
     2101 gcaggccctc gccgcgaagc acgagctcgc cgtgctggag acgtccagcg tcgcggcgtc
     2161 gcgggtgagc gtcgcggcgc gcgccggggt gatggaggtg cggttcgaga agatccccaa
     2221 gcggggtctc tgggtccacc tcaccattcc cgggctcctc gccacctccg gcctgaggct
     2281 gcgccgcgag cctttcgtgg cgttgggggc gagggagatc gagaccggcg acgagtcgtt
     2341 cgaccagacg ttcctcgtcg aaggatcggc cgggctggtg atggcgctgc tcgactccaa
     2401 ggcccgccgc ctgctgctcc agatcaacgt cgagggggag ctcgagatcc gcggcggcgt
     2461 ggtgagcgtc cgggtgaccg acacgacgct cccccagctc ctccccctcc tcctcgagct
     2521 cggccagcgg ttcacgacgg gctcggacac tgtgcgccgc ctggccgcaa gcgcccgcga
     2581 ggaccgcgag gctggggtgc ggctgcgcag cctgctgctg ctcgagcgcg agttcccccg
     2641 cgagcaggtc acccgcgaca cgctgcgcgc cgcctgctcg gacccggccc cgcagatccg
     2701 cctgcgcgcc gccatcgcgc gcggggccga gggccgcgaa accctcatgg cgctcgcgga
     2761 ggacccggag gtggacgacg actggagcgc gcaggccgtc tcgaccctgg gacgacaact
     2821 gccgcacgag cgcacgcagg agatcctccg gcagtccctg cggcggcggc ggctcgggac
     2881 cgcccacgcc tgcctcgcgg ctctcggcca gtggcgcggc gcggcgctcg acgagctggt
     2941 gaaggtgctg gagcgcgagc cggacgctct ggccgccgcc gcggccctgg ccctgggcga
     3001 gagcggagag gccgccgccg agccgccgct gctccgtgcc ctccagcacg gcagcgcgga
     3061 gatccggtcc gccgcggcga ccgcgctggg ccgcgtgggg acggcggcgg ccgtgctgcc
     3121 gctcaaggag gccgccgaag aggaccggga gctccgccgg gtggcgcgcc agtcggtcgc
     3181 cgagatccag tcccgtatcg agggcgcctc ccctggccag ctctcgctgg cgcagtccga
     3241 agcggggcaa ctgtcgctgg ccaccgaccc gtccgggcag ctctcgctgc ggtctgagag
     3301 gccggtggcc tgaggtgacc ccggcacgat ctgggagtgg gtcatccccg tcggattcgg
     3361 tgtcgcagcg ttctggagcc tcggccgcaa ccgccggcgc ctggagagct ggcggagcgc
     3421 cgtcgccgcc tgcggcctga aactggtcga ggcctccagt gtgacgaaga cgaagctcca
     3481 cctgctcgtg cgggcggacc ggatcgaggt ccgtttcgag cacgccagct accagctcac
     3541 ccgggtgatc gtcgagctcc ccgctctgcc gtggttcagc ggcgtgagaa tccgcggcga
     3601 cgactggaaa cccttcggag cccgcgagat cgaagtcggg gactgggtct tcgacacccg
     3661 gttcttcgtc gaggggccgg aacggctcgt agtgcggatg ctcgacgccg agatgcgccg
     3721 cctgctgctc gaagccggca gcaagggcga tctggagatc cgcgagggca ctctccaggt
     3781 ggtcgcgctc gatcatcgcc tgcccgaggt cctgcccatc ctcctggagc tcgcccagcg
     3841 gttcatgcgt cagtccacga atgccgcgcc cggcttggcc gcgaacgccc gccgcgatcc
     3901 cgaacccggc gtacggctcc gcaacctgat ccacctggcc cgtgatttcc gggacgagca
     3961 gctcacccac gagaccctcc gcgccgcctg ccgggacccc agcccccagg tccggctgca
     4021 ggcgggcacg gccttggggg cggaggggcg cgacgtcctg cgcgagctgg cggaggacgc
     4081 ggagggtacg attgaggacg ccatcagctc cctggcggtg gcggcactgg gcgccgagct
     4141 gccgctggag cgcgtgcagg cgatcctccg ccgctccctg cgagaccgcc gtatccagac
     4201 cgcccgcgtc tgtctggaga ggctcggccg cagcggcgac acgacggccg tccccaccct
     4261 ggcgaagatc ctggccctcg aaaaggacga gcttgccatc gccgcggccg tggccctggg
     4321 tatcaccggg gacgaggccg ccgagccgcc gctgatccag gccctcgacc gcgacgacaa
     4381 cgggctgggg gtggccgcgg cgaacgcgct ggcccacatc ggctcggcgg cttcggtgct
     4441 ccccctcaag gaggcggccc gctcgggcga ccaggccctc cgccaggccg cccgccaggc
     4501 ggtcgccgag atccagtccc gttttcatcc cggcgcctct cccggccagc tctcgctcgc
     4561 ccaagccgag gcgggccagc tctcgctcgc caccgacccg gccgggcagc tctcgctccc
     4621 gccccaggag cccgaaaagg ggcctggggg ctgagagctc cgatgctaca ctcccgcgct
     4681 ggtgagtgac gggaccccct ctccgccccg gcgccgggct ctcggcacgg tcttcctgac
     4741 gatcctcctc gacctcatcg ggttcgggat gatcctgccg ctgctgccct tctacgccca
     4801 ggagctcgac gccaccccgt tccagatcgg cctcctcttc gcctcgtaca gcctggccca
     4861 gctcgccttc gcgccgctcc tcggacgggt ctcggaccgg gccggccgcc ggccggtgct
     4921 gctggcctcg atcctgggga gctgcggcgc ctacgtcctc ttctgggccg ccggctccta
     4981 tgccctgctg ctcgtagccc gcaccctgtc cggagtggcg gcgtccaact acggcatcgc
     5041 ccaggcctac gtggcggacg tcaccccgcc cgaggggcgc tccaaggcca tggggctggt
     5101 gggggcggcg ttcggactcg gcttcatcat cggcccggcg
//
LOCUS       DASPJI010000690         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_44567, whole genome shotgun sequence.
ACCESSION   DASPJI010000690 DASPJI010000000
VERSION     DASPJI010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44567"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1621)
                     /locus_tag="VEL74_19520"
     CDS             complement(113..1621)
                     /locus_tag="VEL74_19520"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HYG64779.1"
                     /translation="MRRTAAALVVLALLGLADACWLEPRVLLFRDAARIHLDAPPVTL
                     VHLSDLHISNDPPLLRRLLNKVRETAPNLIVISGDLIQDLPDAERRARHTAATAAFIA
                     QLRRVAPVLAVQGHSEHQGDVIAALSDAGIEWLSNEGRFLPTGGVGESGGILLLGVNQ
                     QVGQDALVTPWPSPFRTMRRQGRWHYGARLQEIRRNFYSHYDPAPRGRTDAGGPLTWS
                     GYEAVCDVLLADEDSAAALVVHSRYVLGEDRLYRLRRVRPEHGLPGTFYLVPHGTTLE
                     GDIDTGVEPEPGRWYRMRLRTEVDPGVVRVLAKVWPVDGPEPKEWQARAEDRSPYRVE
                     SGTAGLWASGEGEVLYKDLRVTGADGRVLLDEPLAGSREPAGFREGLRGTRLEVALAR
                     SPEVPPGTPRVVLSHVPEIVREASRRGLDAVIAGHTHGGQVRLPFFGALTTRSALGPY
                     YDFGRYEFPAPNRRGTTTLYLNGGVGMSMLPVRFWCPPRWAVLRLGSEPAGR"
     gene            complement(1624..>2036)
                     /locus_tag="VEL74_19525"
     CDS             complement(1624..>2036)
                     /locus_tag="VEL74_19525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HYG64780.1"
                     /translation="GHLRYLLFYLLAGYAATIAHAWSAPFSMLPAVGASGAISGVLGA
                     YLFLHPKARIVTVIWFFIFIRFIQIPAIVYLPIWFALQLFSGVASLTTATADNAGGVA
                     WFAHIGGFVAGPVLLALLGGFRKQRRPATAPGLR"
BASE COUNT          311 a          707 c          777 g          241 t
ORIGIN      
        1 tcggcggctc gttcccacgc gcatcactcc ggcctctcca gaagaaggat cacccatcct
       61 gcggtgccgt ccattaagtg cgggacaggt ggggaaagcg ggacaaccgg cttcaacgcc
      121 cggccggctc gctcccgagc cgcaggacgg cccaacgcgg cgggcaccag aatcggacgg
      181 ggagcataga cataccgacg ccgccgttca agtagagggt ggtcgtcccc cgccggttgg
      241 gggccgggaa ctcgtagcgg ccgaagtcgt agtagggtcc gagggcggag cgggtggtca
      301 gggcgccgaa gaaggggagg cgcacctggc cgccgtgggt gtggccggcg atcaccgcgt
      361 ccaggccccg gcgggaggcc tcgcggacga tctccggcac gtgggagagg acgacccgcg
      421 gcgtccccgg cggcacctcc gggctgcgcg ccagggccac ctcgaggcgc gtcccccgga
      481 gcccctcgcg gaaaccggcc ggctccctgg agccggcgag cggctcgtcc agcagcaccc
      541 ggccgtccgc gccggtcacg cggagatcct tatagaggac ctccccttcg ccggaggccc
      601 agagtcccgc ggtgcccgac tccacccggt aaggggagcg gtcctcggcc cgcgcctgcc
      661 actccttcgg ctcgggcccg tccaccggcc agaccttggc cagcacccgc accacgccgg
      721 ggtccacctc ggtccgcagg cgcatccggt accagcgccc cggctccggc tcgacgccgg
      781 tgtcgatgtc gccctcgagg gtcgtgccgt gggggacgag atagaaggtg ccgggcaggc
      841 cgtgctcggg gcggacgcgg cgcagccggt acagccggtc ctcccccagg acgtagcggc
      901 tgtggaccac cagtgccgcg gccgagtcct cgtccgccag gagcacgtcg cagaccgcct
      961 cgtagccgct ccaggtgagc ggcccgcccg cgtccgtccg acctcggggg gccgggtcgt
     1021 agtggctgta gaagttccgg cggatctcct ggaggcgggc gccgtagtgc cagcgcccct
     1081 gccgccgcat cgtccggaac ggcgacggcc agggggtgac cagggcatcc tgccccacct
     1141 gctggttgac ccccaggagg aggatgccgc cgctctcccc gaccccgccg gtcggcagga
     1201 agcgtccctc attggacagc cactcgatgc cggcgtccga gagggcggcg atgacgtccc
     1261 cctggtgctc gctgtgcccc tgcaccgcga gcaccggagc cacccgccgg agctgggcga
     1321 tgaaggcggc cgtggccgcg gtgtgccgcg cccgccgctc ggcgtccggc aggtcctgga
     1381 tgaggtcgcc gctgatgacg atcaggttgg gcgcggtctc ccggaccttg ttcagtagac
     1441 gccggagaag cggcgggtcg ttcgagatgt ggagatcgct gagatggacc agggtgaccg
     1501 gcggcgcgtc cagatggatg cgggccgcgt cccggaagag gaggaccctg ggctcgagcc
     1561 agcaggcgtc ggcgaggccc agcagggcca ggacgacgag ggccgccgcg gtccgccgca
     1621 cggtcagcgc agcccgggag ccgtggccgg ccggcgctgc ttccggaagc cgcccaggag
     1681 ggcgagcagc accggcccgg cgacgaagcc gccgatgtgg gcgaaccagg cgacgccgcc
     1741 ggcgttgtcg gccgtggccg tggtcagcga ggcgacgccc gagaagagct gtagcgcgaa
     1801 ccagatgggg aggtagacga tggcggggat ctggatgaag cggatgaaga tgaagaacca
     1861 gatcacggtc acgatccgcg ccttcgggtg gaggaagagg taggccccca gcacccccga
     1921 gatggcgccg ctcgccccca cggccggcag catcgagaag ggggcggacc aggcgtgggc
     1981 gatggtggcg gcgtacccgg cgagaagata gaagaggagg tagcggaggt gcccca
//
LOCUS       DASPJI010000691         2907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_612086, whole genome shotgun sequence.
ACCESSION   DASPJI010000691 DASPJI010000000
VERSION     DASPJI010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612086"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VEL74_19530"
     CDS             complement(<1..761)
                     /locus_tag="VEL74_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HYG64781.1"
                     /translation="MKPFTVGLCLILAACGSVPPPAPSAGVPPTLSKDVAETKETFMA
                     RARAQIEANEQVRPDRRKARNVILFVGDGMGVSTITAARILEGQQKGQRGEENSLSFE
                     RFPYLGHSKTYQANQQVPDSAPTMTSIITGSKANDGSLSVAPNVLADDFAAAALPENR
                     LRTLLEEAESRGLWTGVVSTATVTHATPGACYAHTPNRGAESDADLARISPEAAAAGY
                     PDIARQLVEFQLRPVTMNGRQSRGLEVAMGGGRSK"
     gene            874..1632
                     /locus_tag="VEL74_19535"
     CDS             874..1632
                     /locus_tag="VEL74_19535"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HYG64782.1"
                     /translation="MRKIPSTNFFVTALFAAALLTGCGSSGIDGILGGGSGSSGGSTT
                     NYPSSSRANEVQGTVERVDTLDRRIIVDGDSTYRTDLRNGNEDNEIVLYYDDRTTVEY
                     RGETFRPQDLEPGDRIRADVGQSGSRLMVDQIEVLYDATSGSGSSSGSIGNYDDDRRD
                     SELRGTVRYIDTRDRTLEIQPRNSGFTTNGSGVVIVRYDTNTVVEYEGRRYTPDNLER
                     GDEVEIEVREGLNGQFLAEEILVVQDSRASLGNR"
     gene            1737..2903
                     /locus_tag="VEL74_19540"
     CDS             1737..2903
                     /locus_tag="VEL74_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64783.1"
                     /translation="MSVEALSATRSAPARWLGLVPKWLAVSLATVLFLMLTAELIPVW
                     QSTSPTPAAIVIALAVGLVASVSWTLRETRVDSARMYYYLQVIVRFGLSYIFIGYGLS
                     KLLGIQFGPPTFFQLDKPVGTLPGIQLTWLFFGYSFAYGAFIALSQIACSILLAFRRT
                     YLLGACLLLPVIGNIVFVNFTHGIPVKLNSTIYLIATLSLLVPELPRFKALFWDHAPV
                     PGKSFPALSPRYRGAVPLATAAFLAGAFAYQGWVLNDLAAPTRPGPVAQPVAGAWRVE
                     RCEPMAPTATPLVACVDGPDRWQKVLFENRYGGKDGFIKFAEGYVITAYKVDPARRSL
                     TFESTKGRSEPFTGTYRIDRQADGDRLVLEGKLGPDPVRIHLVKERYRFPSITG"
BASE COUNT          446 a         1042 c          921 g          498 t
ORIGIN      
        1 aacttcgacc ggccgccccc catcgccacc tcgagccccc ggctctgcct gccgttcatc
       61 gtcaccggcc ggagctggaa ctcgacgagc tggcgcgcga tgtccggata gccggcggcc
      121 gcggcctcgg gactgatccg ggcgaggtcg gcgtcggact ccgcgccgcg gttcggcgtg
      181 tgggcatagc aggcgcccgg agtcgcgtgg gtgacggtgg cggtcgagac gacgccggtc
      241 cacagccccc gggattcggc ctcctcgagc agggtacgga ggcggttctc gggcagcgcg
      301 gcggcggcga agtcgtccgc cagcacgttc ggggcgaccg acaggctgcc gtcgttggcc
      361 ttgctgccgg tgatgatcga tgtcatcgtc ggcgcggagt ccggcacctg ctggttcgcc
      421 tggtaggtct tcgagtgccc cagatagggg aagcgctcga agctcaggga attctcctcg
      481 ccccgctgcc ccttctgctg cccctccagg atgcgggcgg cggtgatcgt cgacaccccc
      541 atgccgtcgc cgacgaacag gatgacgttg cgggccttgc ggcggtccgg ccgcacctgc
      601 tcgttggcct cgatctgcgc ccgcgcgcgg gccatgaagg tctccttggt ctcggcgacg
      661 tccttggaaa gggtcggcgg cacgccagcc gaaggggcgg gcggcgggac ggagccgcaa
      721 gctgccagga tcagacagag gccaaccgtg aacggcttca tcgcatctcc tcctctcgga
      781 atcagcccct tagcttaccc cgagccgggc cggcacgagt cctgcagtct actgccgtgg
      841 gatgggcgcg actaccttta ggaggtacta accatgagaa aaatcccatc gacaaatttc
      901 ttcgtgacgg ccctttttgc cgccgccctc ctcaccggct gcggctcgtc cggcattgac
      961 ggaatcctcg gcggcggctc cggcagctcc ggcggctcta ccaccaacta cccctcgtcc
     1021 tcgcgggcga acgaggtcca gggcaccgtc gagcgcgtcg acaccctcga ccgccgcatc
     1081 atcgtcgacg gcgacagcac ctaccggacc gacctgcgca acggcaacga agataacgag
     1141 atcgtcctct actacgacga ccgcaccacg gtcgagtacc ggggcgagac cttccggccg
     1201 caggacctgg agcccggtga ccgcatccgc gccgacgtcg ggcagagcgg cagccgcctg
     1261 atggtcgacc agatcgaggt gctctacgac gccaccagtg gcagcggcag ctcgagcggc
     1321 tcgatcggca actatgacga cgaccgccgc gactccgagc tgcgcggcac cgtccgctac
     1381 atcgacaccc gcgaccgcac gctggagatc cagccccgga attcgggctt caccaccaat
     1441 ggctccggcg tggtgatcgt gcgctatgac accaacaccg tggtcgagta cgaggggcgc
     1501 cgctacaccc ccgacaacct ggagcgcggc gacgaggtgg agatcgaggt ccgcgagggc
     1561 ctcaacgggc agttcctggc cgaagagatc ctggtggtcc aggattcccg cgcctcgctc
     1621 ggcaaccgct gagctttcga atcaccgaat tatcagaagg gcggagagga tcctctccgc
     1681 cctttctgtt tccatcctcc gtgctacagt ggctgcaaac actggaggat ttccggatga
     1741 gtgtcgaggc cctctccgcc acccgctccg caccagctcg ctggctcggt ctggtcccca
     1801 aatggctcgc cgtctcgctg gcgaccgtcc tcttcctcat gttgacggcg gagctgatcc
     1861 cggtatggca gtccacctcg ccgaccccgg ccgccatcgt catcgctctg gccgtcggcc
     1921 tggtggcctc ggtctcctgg acgctccggg aaacgcgggt cgactccgcc cgcatgtact
     1981 actacctgca ggtgatcgtc cgcttcggcc tgtcctacat cttcatcggc tacggcctgt
     2041 ccaagcttct cgggatccag ttcgggccgc ccacgttctt ccagctcgac aagccggtgg
     2101 gcaccctgcc gggcatccag ctcacctggc tgttcttcgg ctattcgttc gcctatggag
     2161 ctttcatcgc cctatcgcag atcgcctgct ccatcctcct cgccttccgc aggacctatc
     2221 tgctgggcgc ctgcctgctg ctgccggtca tcggcaacat cgttttcgtc aacttcaccc
     2281 atggcatccc ggtgaagctc aattcgacga tctatctgat cgcgaccctg tcgctcctgg
     2341 tccccgagct gccacgcttc aaggcgctct tctgggacca cgccccggtg ccgggcaagt
     2401 ccttccccgc cctctcccca cgctatcggg gagcggtgcc gctggccacg gccgccttcc
     2461 tcgccggcgc cttcgcctac cagggctggg tgctgaacga tctcgcggcg ccgacccggc
     2521 ccgggcccgt ggcgcagccg gtggcgggcg cctggcgcgt cgagaggtgc gagccgatgg
     2581 ctccaacggc aacgccgctg gtggcctgcg tcgacgggcc tgaccgctgg cagaaggtgc
     2641 tgttcgagaa ccggtacggc ggcaaggacg gattcatcaa gttcgccgag gggtacgtca
     2701 tcacggccta caaggtggac ccggcgcgcc ggagcctcac cttcgaatcg accaagggcc
     2761 gctcggagcc gttcaccggc acctaccgga tcgaccggca ggccgacggg gaccggctcg
     2821 tgctcgaagg aaagctcggg ccggaccccg tgcggatcca cctggtgaag gagaggtacc
     2881 gcttcccctc gatcaccggg tgaaacg
//
LOCUS       DASPJI010000692         9990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_676819, whole genome shotgun sequence.
ACCESSION   DASPJI010000692 DASPJI010000000
VERSION     DASPJI010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9990)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676819"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VEL74_19545"
     CDS             <1..746
                     /locus_tag="VEL74_19545"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYG64784.1"
                     /translation="ATGRKVAVLGESLARAVWGGRDPIGQRFLRGSEPDWVTVVGVAA
                     DSRFEGLETAPRPVLYRPFAQQPIPLGYLAVRTAKDPLSLAAAVRREVAAVDSTVAVH
                     DVATLEQRLSGSVAERRFNVTLLGLFAVLAMVLAGVGLYGVLSYLVTERTREMGIRMA
                     LGAERRRILGLVIRQGVMTAAAGAAVGLGTALVLGRILASALFGVTATDPVTFSVIPA
                     VLLATAVLASWLPARRATRVDPGVALRAE"
     gene            912..1697
                     /locus_tag="VEL74_19550"
     CDS             912..1697
                     /locus_tag="VEL74_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64785.1"
                     /translation="MTVQSPPPPPSYGGPVTPGAPKKKGLSPLAWVGIGCGAILVLGF
                     IVMAVGGFLVARKAKQYADNPEMAAVELMVAANPDIELVSKDEDAKTVTLRNKQDGET
                     ITVSLEDVKNGNISFETSEGEASIQMNEEGMTIKDGEGNTAFQAGASTSAPKDLPDWV
                     PVYPGGQIQGNMTSNTAEQKTASFSLTTQDSVDDLVKFYEEQLTAKGLEIEQTTTGTG
                     ADGGSTGMISAKSSDDVRNVIIMVSSATEGTGASAQVTYIEKK"
     gene            complement(1742..2860)
                     /locus_tag="VEL74_19555"
     CDS             complement(1742..2860)
                     /locus_tag="VEL74_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003165935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HYG64786.1"
                     /translation="MAAAVLAAADSTAPRPRLRDLGISPGIFQPGPLNAITDVAGVRV
                     GHRTLVRGDAVRTGVTAILPHGGNLFQQKVPAGVSVGNGFGKAAGFLQVRELGNLETP
                     IVLTNTLAVGTALEAVVAWTLEQPGNEAVRSVNAVVGETNDGFLNDIRARPVTAADVR
                     AAIMAAKTGAVEEGSVGAGTGTMALGWKGGIGTASRVLPASLGGYTVGALVQTNYGGV
                     LTIDGVPVGERQGRFYFRDDVRKEPGDRGDGSCMIVLATDAPLSDRNLERLANRAFLG
                     LARTGSFLSNGSGDFAIAFSTRNSIPFEPKERVRQVEELHNDATSPLFLAAVEAVEEA
                     VYNSLTRATDVTGHEGHKGEAIPIEELRKLLAERREGR"
     gene            complement(2887..3309)
                     /locus_tag="VEL74_19560"
     CDS             complement(2887..3309)
                     /locus_tag="VEL74_19560"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG64787.1"
                     /translation="MRQFVIDASVAVKWYLPEPYSDKAEAVLADSGALLAPDHLYLEV
                     ASVLWQRGLRGEIDPATAGQIVAELRKVPIELRHTTELVTDALALALESGCTLEDSLY
                     LALAVQASCPVVTADRRFHDALKGGPLGAHVLWIGDFV"
     gene            complement(3310..3540)
                     /locus_tag="VEL74_19565"
     CDS             complement(3310..3540)
                     /locus_tag="VEL74_19565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HYG64788.1"
                     /translation="MLKLILQDVTPETVEELRRRAEPKGRTVEAEARALLEAALGLSR
                     GRALRAARGLRESLAGQTFSDSAELIRAARER"
     gene            complement(3607..4143)
                     /locus_tag="VEL74_19570"
     CDS             complement(3607..4143)
                     /locus_tag="VEL74_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYG64789.1"
                     /translation="MAQRDGQNDIEKLRDLIKDVRVAMLTTVDEDGSLRSRPMATQEM
                     ELDGELWFFTGAGSPKVREVQREQHVNVSYANPDDQQYVSVSGTAQVVHDRAKAEELW
                     NPILKAWFPKGLDDPDLALLRIRVEKAEYWDSPSSKMVQLAGFVKAVTTGKRYEPGPG
                     EHGQLELAGTTGATRNEG"
     gene            complement(4180..4485)
                     /locus_tag="VEL74_19575"
     CDS             complement(4180..4485)
                     /locus_tag="VEL74_19575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64790.1"
                     /translation="MSYQQSGKQGVVTDSQKRDGYRNEYDPAPSSAPVAGAFGREGGP
                     EGTRPDGPQELPVADRASVLAADEEDDDLEDDDDLDDDDDDFDDDEDDDDLDEEDEA"
     gene            complement(4601..7510)
                     /locus_tag="VEL74_19580"
     CDS             complement(4601..7510)
                     /locus_tag="VEL74_19580"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HYG64791.1"
                     /translation="MRPQRLVVLSFLALVAMAASASAQSLVRVSPGGDLQAAINSVAD
                     GGVIEMAGGTYPSPANGWLIANEGRGFTIRAAQGAQVVLDGGRSRRILRFENGTRNRG
                     RLVTFDGLTFRNGAGDGGAAVNLIQAEAVFRDCVFEDNAASPNSAYGAVRAFTGTDVT
                     FARCTFRGNQARVRGGALSVERASAVIQGCTFRDNRVNQAGHDPGSAGGAVYILDSTV
                     RVADSHFEGNEAGWVGGALYAIGVWADPVSTPRADVRVTRSTFLDNRAEPHGCCNPPG
                     PTTGGAVHAEDQTTMTLEGSRFVGNEALHGGAVSGYRAIVEVRGSVFQGNRSLTAAGG
                     GGAGAGGAINMTSNEFADASTGNGTINRRPARLTATDSWFQGRFGATTTAAHVGGCLL
                     AGGDASRMYGENGVAAAGGLADNRAVVVLRNTVFFDCDVERTADGQGGFGGGIQADLT
                     DLTLEDSMVLHSDARGTNAGGGGMSVSRDSHSRIARTTFARNTGEARAGAVDLGGSTL
                     ELTASRFLANEVSPGVNEGTNQSLGAALYSIPLLAPANPARARNVAGVVSNTLFSDNA
                     GVPVFDIDPQSGPINDVRYDGNQFFSSRPVYVNTLTDPGRQGSSVAQLNALVVSRPGR
                     PSTDKSLVANQQLNAAPRVGDLVAVPPGVPVGAPSSPGNAFLAYAWTGGRSATLNGQA
                     LSGRSGVMEVAPGTYTLAVDGVTVDQTTVGASSCTTGSRLCLRNDRFRLEVSWRTRQG
                     ETGSGQAVRLTGDTGYFWFFNSANVELVVKVLDGRALNGHFWVFYGALSDVQYTLTVT
                     DTATGRVKTYNNPQGQLASRADTSAFPAGGAAADAATASLWEEDVVAAPEPVVTALDN
                     ACAAGATQLCLNASRFRVQVTWEAQGSSGSGQAVPLTSDTGYFWFFDPANVELVVKVL
                     DGRALNGHFWVFYGALSDVRYTVTVTDTVTGRSKTYTNPAGTLASRADTQALPSQ"
     gene            complement(7553..8089)
                     /locus_tag="VEL74_19585"
     CDS             complement(7553..8089)
                     /locus_tag="VEL74_19585"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYG64792.1"
                     /translation="MSADGFRVQQIDHVEVFVPDRHEAARWYEEVLGLRILTRHEDWA
                     EHPQGPLMISSDDGVTMVALFQGEPPGASLSAGAVRVAFRVDGPGFLRFLDRAPGLPL
                     HDQQGQRATPRVRDHGKAFSAYFCDPWGNRYEVTTYDHAAIGAAWPGARLRPATGNNP
                     LPSPVLTAASRRPCRTSC"
     gene            complement(8107..9084)
                     /locus_tag="VEL74_19590"
     CDS             complement(8107..9084)
                     /locus_tag="VEL74_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HYG64793.1"
                     /translation="MSGNARRWDFPSPAAPGSLGGMGGGHSHSHHHHHGGSAAGGNAR
                     RLSLTLGLAAVYMVAELVGGLMTGSLALLADAGHMLSDVGALALSLFALWISRRPASS
                     RRTWGYYRTEILAALVNGATLIAVSVYVFFEAWERFHNPPRVDAPVMMGIAVGGLLVN
                     LAGLWILSGGRDESLNIRGAWLHVLTDALGSVQAIAAGALIWAYGWAWADPVASVLIG
                     LLVIYSAWALLREAVEVLMEAAPAHIDVDEVRDAIISLPGVVAVHDLHVWTITSGMES
                     LSAHLVVEEGREGRALLARVRATLHDRFGIDHITVQLEPEEFEPCRSRV"
     gene            complement(9194..>9990)
                     /locus_tag="VEL74_19595"
     CDS             complement(9194..>9990)
                     /locus_tag="VEL74_19595"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HYG64794.1"
                     /translation="GGGYGGGESGRGGGSMGGYYGGGQDYDRQGGGAMGGFNRSQGGQ
                     GGGQGTGSYSGYGGGSSQGGGGGMSGGGMERSGGGGMFGGFSSFGTGNEQPWQRQGPH
                     TGRGPEGYKRSDERIHEEVCERLTQHGDVDASRVRVRVEDGEVTLEGSVTSRHEKRVA
                     EDILESLPGVKDVHNHLRVQQQGGNGREGQTQQGQTMAATTAGLTSAGATSGAGSTGG
                     MTAGSSGTGSTGSSTGSAAGATSGSASGSTSGEMGTESSRSRGRRS"
BASE COUNT         1416 a         3530 c         3617 g         1427 t
ORIGIN      
        1 ccgccaccgg ccggaaggta gcggtcctcg gcgagagcct ggcgcgggcg gtgtggggcg
       61 gccgcgaccc gatcgggcag cgcttcctgc gcggcagcga gccggactgg gtgaccgtcg
      121 tcggggtggc cgcggacagc cgcttcgagg gtttggagac ggcgccgcgg ccggtcctct
      181 accggccgtt cgcccagcag ccgatccccc tggggtacct cgccgtgcgc acggccaagg
      241 acccgctctc cctcgccgcc gccgtccgcc gcgaggtggc cgccgtggac tccaccgtgg
      301 cggtgcacga cgtcgccacg ctcgagcagc gcctgtccgg ctcggtggcc gaacggcggt
      361 tcaacgtgac gctcctcggg ctgttcgccg tgctggcgat ggtcctcgcc ggcgtcggac
      421 tctacggcgt cctctcttac ctggtgaccg agcgcacgcg ggagatgggg atccgcatgg
      481 ccctgggcgc ggagcggcgg cgcatcctgg ggctggtgat ccggcagggg gtgatgaccg
      541 ccgccgccgg cgccgccgtg ggtctcggga ccgccctggt gctgggccgg attctggcga
      601 gcgccctctt cggcgtcacg gcgaccgacc cggtcacctt ctcggtgata cccgccgtcc
      661 tcctggcgac ggccgtcctc gcctcctggc tgccggcccg gcgcgccacc cgggtggacc
      721 ccggcgtggc gttgcgggcg gagtgaggga aggctgaaac gaatccccgg cctccggcgc
      781 ctgagaaggg gtccggcctc caggtgaggg ggtcgaggga cccgctccgt tcaacagggg
      841 cgggcggaca tggtatcgtt ccgccagctt agaacaactt tgcaactctc tcagtcagaa
      901 ggagattcat gatgactgtc cagtctcctc cgccgccgcc gtcctacggc gggcccgtca
      961 ctcccggcgc tcccaagaag aagggcctca gcccgctcgc ctgggtcggt atcggctgcg
     1021 gcgcgatcct cgtcctcggt ttcatcgtca tggcggtcgg cggcttcctg gtcgcccgca
     1081 aggccaagca gtacgccgac aaccccgaga tggccgcggt cgagctcatg gtcgccgcga
     1141 acccggacat cgagctcgtc agcaaggacg aagacgccaa gaccgtcacc ctgcgcaaca
     1201 agcaggacgg cgagacgatc accgtcagcc tggaggacgt caagaacggc aacatcagct
     1261 tcgagacctc cgagggcgag gcgtccatcc aaatgaacga ggaggggatg acgatcaagg
     1321 acggagaggg caacaccgcc ttccaggcgg gtgccagcac cagcgccccc aaggacctgc
     1381 ccgactgggt gccggtctat ccgggtggac agatccaggg gaacatgacc tccaacactg
     1441 cggaacagaa gaccgcgagc ttctcgctga cgacccagga ctcggtggac gacctcgtca
     1501 agttctacga ggagcagctc acggccaagg ggctggagat cgagcagacc accacgggta
     1561 ccggcgccga cggcggctcg accggcatga tctccgccaa gagcagcgac gacgtgcgca
     1621 acgtcatcat catggtcagc tccgccaccg aggggaccgg ggcgtccgcc caggtcacct
     1681 acatcgagaa gaaataggct ccccgcctgc caggcgcgcc ccctccccga gggggcgcgc
     1741 ctcacctccc ctcgcgccgc tccgccagca gcttccgcag ctcttcgatc gggatcgcct
     1801 cgcccttgtg cccctcgtgg ccggtgacgt cggtcgcccg ggtgagggag ttgtagaccg
     1861 cctcctccac cgcttcgacg gcggcgagga agagggggct cgtggcgtcg ttgtggagct
     1921 cctcgacctg gcggacccgc tccttcggct cgaaggggat cgagttccgc gtggagaagg
     1981 cgatggcgaa gtcgccggag ccgttggaga ggaacgagcc ggtgcgggcg aggcccagga
     2041 aggcgcggtt cgccagccgc tccaggttgc ggtcggagag cggcgcgtcg gtggcgagca
     2101 cgatcatgca ggagccgtcg ccgcggtccc cgggttcctt gcggacatcg tcccgaaagt
     2161 aaaagcgtcc ctgccgctcg ccgaccggga cgccgtcgat ggtcagcacg ccgccatagt
     2221 tggtctgcac cagcgcgccg acggtgtagc cgccgagact cgccggcagc acccgcgagg
     2281 cggtgccgat cccccctttc cagcccaggg ccatggtgcc ggtgccggcg ccgacgctcc
     2341 cctcctccac cgctcccgtc ttggcggcca tgatggcggc gcggacgtcc gccgccgtca
     2401 ccgggcgcgc gcggatgtcg ttgaggaaac cgtcgttggt ctcgcccacc acggcgttga
     2461 ccgagcgcac ggcctcgttg cccggctgct cgagcgtcca ggccaccacg gcctcgaggg
     2521 cggtgcccac ggccagggtg ttggtgagga cgatcggagt ctccaggttc cccagctcgc
     2581 ggacctggag gaatcccgcg gccttgccaa agccgttgcc cacggacacc ccggccggca
     2641 ccttttgctg gaagaggttg ccgccgtgcg ggaggatggc cgtcaccccg gtgcgcaccg
     2701 cgtcgccgcg caccagtgtg cggtggccga cgcggacgcc ggccacgtcg gtgatggcgt
     2761 tgagcgggcc gggctggaag ataccgggag agatccccaa gtcacggagg cgcggccggg
     2821 gagccgtgct atcggcggcg gcgaggacgg ccgccgccag gaccgcggcg gcgagggccg
     2881 gccggcctag acgaagtccc cgatccacag cacgtgggct cccaggggtc cgcccttcaa
     2941 ggcgtcgtgg aaccggcggt cggcggtcac caccggacag ctcgcctgca ccgccagcgc
     3001 gaggtagagg gagtcctcca gggtgcagcc gctctccagg gcgagggcca gggcgtcggt
     3061 gacgagctcg gtggtgtgcc gcagctcgat ggggaccttg cgcaactcgg ccacgatctg
     3121 cccggccgtg gccgggtcga tctctccccg cagcccgcgc tgccagagca ccgaggcgac
     3181 ttccagatag aggtggtccg gggcaaggag ggcgccggag tcggcgagca cggcctcggc
     3241 cttgtcggag tagggctccg gcagatacca cttgacggca acgctggcat cgatcacgaa
     3301 ctgccgcatt cagcgctccc gggccgcgcg gatcagctcg gcgctgtcgg agaaggtctg
     3361 tccggcgagg ctttcgcgca ggccgcgggc cgcacggagg gcacggccac gggagagccc
     3421 cagcgccgcc tcgaggaggg cacgcgcctc cgcttctacg gtgcgcccct tgggctcggc
     3481 gcgccggcgc agctcctcga cggtttccgg ggtgacatcc tggagaatca acttcagcat
     3541 ggggcgaagc atacgccagg gaggcgccgc gcgcaccggt gcgcgtggcg cctccccggc
     3601 ctccggtcag ccctcgttac gggtcgcccc ggtcgtgccc gccagctcca gctgcccgtg
     3661 ctcgcccggg cccggctcgt accgcttgcc ggtggtcacg gccttcacga agccggcgag
     3721 ctgcaccatc ttgctggacg gcgaatccca gtactcggcc ttctccaccc ggatgcggag
     3781 cagggccagg tcgggatcgt cgagcccctt ggggaaccag gccttgagga tcgggttcca
     3841 cagctcctcg gccttcgccc ggtcgtggac cacctgcgcc gtccccgaga cggagacgta
     3901 ctgctggtcg tccggattgg cataggagac attcacgtgc tgctcgcgct ggacctcccg
     3961 caccttgggg gagccggcgc cggtgaagaa ccagagctcg ccgtcgagct ccatctcctg
     4021 ggtcgccatg ggccggctgc gcagggagcc gtcctcgtcc acggtggtga gcatggcgac
     4081 ccggacatct ttgatcaggt cgcgcagctt ctcgatatcg ttctgtccat cacgctgggc
     4141 catggtccaa cctcctctct ctcctcggcc aagccggaat caagcttcgt cttcctcgtc
     4201 gagatcgtcg tcgtcctcgt cgtcgtcgaa gtcatcatcg tcgtcgtcga gatcgtcgtc
     4261 atcctcgagg tcgtcgtcct cctcgtccgc cgcgagcacg ctggcgcgat ccgccaccgg
     4321 aagctcctgg ggaccgtccg gccgggtccc ctcgggcccg ccctcgcgcc cgaaggctcc
     4381 cgcgaccggg gcggagctgg gggccgggtc atattcgttg cggtagccgt cccgcttctg
     4441 ggagtcggtc acgaccccct gcttgccgct ctgctggtag ctcatggcac ctcctcgatg
     4501 tgggttctgc gtggtgggcc gagacacgtc caccccacgc cggggaggac ggccggaggc
     4561 cgccaccccg ggacaggagc aatcagcgtg ccgcccggag ctactgggag ggcagcgcct
     4621 gagtatcggc ccggctggcg agggtacccg cgggattcgt gtaggtcttg gaccgccccg
     4681 tgacggtgtc ggtgacggtg acggtgtagc ggacatcgga cagggcgccg tagaagaccc
     4741 agaaatggcc gttgagggcg cggccgtcga gcaccttgac caccagctcg acgttggcgg
     4801 ggtcgaagaa ccagaaatag ccggtgtccg aggtgagggg caccgcctgg ccgctgccgc
     4861 tgctcccctg tgcctcccag gtcacctgga cacggaagcg gctggcgttg aggcagagct
     4921 gggtggcgcc tgccgcgcag gcgttgtcga gcgccgtcac cacgggctcc ggcgccgcca
     4981 ccacgtcctc ctcccagagg gaggcggtgg ccgcatccgc ggccgccccg ccagccggaa
     5041 atgccgaggt gtcggcgcgg ctggcgagct ggccctgggg gttgttgtag gtcttcaccc
     5101 ggccggtggc ggtgtcggtg acggtcagcg tgtactggac gtccgagagg gcgccgtaga
     5161 acacccagaa gtggccgttg agcgcgcggc cgtcgagcac cttgaccacc agctcgacgt
     5221 tggcggagtt gaagaaccag aagtagccgg tatcgcccgt gaggcggacg gcctgccccg
     5281 agccggtctc cccctggcgg gtgcgccagg agacctcgag ccggaagcgg tcgttgcgta
     5341 ggcagagccg ggaccccgtg gtgcacgacg acgcccccac ggtggtctgg tcgacggtca
     5401 cgccgtccac cgccagggtg taggtgcccg gggcgacctc catgacgccg ctccggccgg
     5461 acagcgcctg gccgttgagc gtggccgacc ggccgccggt ccaggcgtag gcgaggaagg
     5521 cgttgcccgg cgaggacggc gcccccaccg gcacgccggg cggcaccgcc accagatcgc
     5581 ccacccgcgg cgcggcgttg agctgctggt tggccaccag gctcttgtcg gtcgacgggc
     5641 ggcccggccg cgacacgacc agggcgttga gctgcgccac gctgctcccc tgccggcccg
     5701 ggtcggtgag ggtgttcacg tagaccggcc ggctcgagaa gaactggttg ccgtcgtagc
     5761 gcacgtcgtt gatcggaccc gactgggggt cgatgtcgaa caccgggacg ccggcgttgt
     5821 cgctgaacag ggtgttggag accactccgg cgacgttgcg cgcccgggcg gggttcgccg
     5881 gggcgagcag ggggatcgag tagagggcgg cccccagcga ctggttggtc ccctcgttga
     5941 cgccggggct cacctcgttg gcgaggaagc ggctggcggt caactcgagc gtggagcctc
     6001 ccagatccac ggcgcccgcc cgggcctcgc cggtgttgcg ggcaaaggtg gtgcgggcga
     6061 tccgggagtg ggagtcgcgg gagacggaca tgccgccgcc gccggcgttg gtcccccggg
     6121 cgtcggagtg gaggaccatg gagtcctcca gggtcagatc ggtgaggtcg gcctggatgc
     6181 cgccgccgaa gcccccctgg ccgtcggcgg tccgctcgac gtcgcagtcg aagaagacgg
     6241 tattgcgcag caccaccacg gcacggttgt cggcgagccc ccccgccgcg gccacgccgt
     6301 tctccccgta catccggctg gcgtcccccc ccgccagcag gcagccgccg acgtgcgccg
     6361 cggtggtggt ggcgccgaag cggccctgga accaggagtc ggtcgccgtc aggcgcgccg
     6421 gccgcctatt gatggtgccg ttgcccgtcg aggcgtcggc gaactcgttc gacgtcatgt
     6481 tgatggcgcc gcccgctccc gcaccacccc cgccggcagc cgtcaaggac cggttgccct
     6541 ggaagaccga gccgcggacc tcgacgatgg cgcggtagcc gctcaccgcg ccgccgtgca
     6601 gggcctcgtt gccaacaaag cgcgagccct cgagggtcat cgtcgtctgg tcctcggcgt
     6661 gcaccgcgcc gccggtggtg ggtccgggag ggttgcagca gccatgaggc tcggcgcggt
     6721 tgtcgaggaa ggtcgagcgg gtcacccgca cgtccgccct gggggtggac accgggtccg
     6781 cccagacgcc gatggcgtac agggcgccgc ccacccagcc cgcctcgttg ccctcgaagt
     6841 ggctgtcggc gacgcgcacg gtggagtcga ggatatagac cgcgccgccg gccgatccgg
     6901 ggtcgtgccc ggcctggttc acccggttgt cgcggaaggt gcagccctgg atgacggcgc
     6961 tggcgcgctc cactgacaac gcgccgccgc gcacccgcgc ctggttcccc cggaaggtgc
     7021 agcgggcgaa ggtgacgtcc gtaccggtga aggcgcgcac cgccccgtag gccgagttgg
     7081 gagaggcggc gttgtcctcg aagacgcagt cgcggaagac ggcctcggcc tggatcaggt
     7141 tgaccgcggc gcccccatcc ccggcgccgt tgcggaaggt caggccgtcg aaggtgacga
     7201 gccggccgcg gttgcgggtg ccgttctcaa accgcaggat gcgccggctg cgcccgccgt
     7261 cgagcaccac ctgggccccc tgcgccgcgc ggatcgtgaa cccccggccc tcgttggcga
     7321 tcaaccagcc gttggcgggc gaggggtagg tcccgcccgc catctcgatc accccgccgt
     7381 cagcgaccga gttgatggcc gcctgcaggt ctccgcccgg cgacacccgg acgaggctct
     7441 gggcggaggc ggaggcggcc atcgccacga gggcgaggaa cgacaggacg acgaggcgct
     7501 gaggtctcat gggcgatcct tccagatgac tgcggggttg accgcgcgca ctttagcatg
     7561 aagtgcgaca gggcctccgc gacgccgcag tgagaacagg ggaagggagg gggttgttcc
     7621 ccgtcgcagg cctaagccgt gctcccggcc atgcggcccc gatggcggcg tggtcgtagg
     7681 tggtgacctc gtagcgattg ccccagggat cacagaagta ggcggagaaa gccttgccgt
     7741 ggtcgcggac acggggcgtg gcgcgctggc cctgctggtc gtggagggga agaccgggag
     7801 cgcggtcgag gaagcggagg aagccggggc cgtccacccg gaaggcgacg cggacggcgc
     7861 cggcggagag gctggcgccg gggggctccc cctggaagag ggcgaccatg gtcactccgt
     7921 cgtcgctcga gatcatgagc ggcccctgcg ggtgctcggc ccagtcctcg tgccgggtga
     7981 ggatccggag acccagtacc tcctcgtacc agcgcgccgc ctcgtggcgg tcggggacga
     8041 agacctcgac gtgatcgatc tgctggacgc ggaaaccgtc tgccgacatg gagatctcct
     8101 ccgggatcag acccgggacc ggcacggctc gaactcctcg ggctcgagct gcaccgtgat
     8161 gtggtcgatg ccgaagcggt cgtggagggt ggcgcggacg cgggccagga gggcacgccc
     8221 ctccctcccc tcctccacca ccaggtgcgc cgacagcgac tccatgccgc tggtgatggt
     8281 ccagacgtgg aggtcgtgca ccgccaccac tccgggaagc gagatgatcg cgtcgcgcac
     8341 ctcgtcgacg tcgatgtggg cgggcgcggc ctccatcagg acctccacgg cctcgcgcag
     8401 cagcgcccag gccgagtaga tcaccagcag gccgatgagc accgaggcca ccggatcggc
     8461 ccaggcccag ccgtaggccc agatgagggc gccggcggcg atcgcttgga cgctccccag
     8521 ggcgtcggtg agcacgtgca gccaggcgcc gcggatgttc aggctctcgt cgcggccgcc
     8581 gctcaggatc cagagccccg ccaggttgac cagcaggccg ccgacggcga tccccatcat
     8641 caccggcgcg tccacccggg gagggttgtg gaagcgctcc caggcctcga agaagacgta
     8701 gacggagacg gcgatcaggg tggcgccgtt gaccagcgca gccaggatct cggtgcggta
     8761 gtagccccag gtgcgccggg aggaggccgg ccggcgagag atccaaaggg cgaagaggga
     8821 cagggcgagg gcgccgacgt cggagagcat gtgcccggcg tcggcgagca gcgccaggga
     8881 ccccgtcatc agaccaccga cgagctccgc caccatatag acggccgcca accccagggt
     8941 cagcgacagc cggcgggcgt tccccccggc ggcggagccg ccatggtggt gatggtgtga
     9001 atgggagtgc ccgccgccca tgccgccaag gctaccaggg gcggcgggtg aggggaaatc
     9061 ccaccggcgc gcgtttccag acatggcccg gctccggctg gcgtcccccc gccggccgga
     9121 ctgccggtgc cgcttgtttc tggagttatc gatcccgggc gggctccaac gagcccgccc
     9181 gttctttttc ggctcagctc ctgcggccgc gggagcggct gctctcggtc cccatctcgc
     9241 ccgaggtgga gcccgaagcc gagccggagg tggcgcccgc ggccgagcca gtgctggagc
     9301 cggtggagcc ggtgccggag ctccccgccg tcatgccgcc ggtggacccg gcgcccgagg
     9361 tggcgccggc gctggtcagc cccgccgtgg tcgcggccat ggtctgcccc tgctgggtct
     9421 ggccctcgcg gccgttgccc ccctgctgct ggacgcgcag gtggttgtgg acgtccttca
     9481 ccccggggag ggactccagg atgtcctccg ccacgcgctt ctcgtgacgg ctggtgacgc
     9541 tcccctcgag ggtcacctcg ccgtcctcca cccgcacccg cacccggctg gcatcgacgt
     9601 cgccgtgctg ggtcagccgc tcgcagacct cctcgtggat ccgctcgtcg gagcgcttgt
     9661 acccctcggg gccgcggccg gtgtgcggac cctggcgctg ccagggctgc tcgttgccgg
     9721 tgccgaagct gctgaagccg ccgaacatgc cgccgccacc gctccgctcc atgccgccgc
     9781 cggacatccc accgccgccg ccctggctgg agccgccacc atagccgctg taggagccgg
     9841 tgccctgacc accaccctgg ccgccctggc tacgattgaa gccgcccatg gcgccaccgc
     9901 cctggcggtc gtagtcctgg ccgccgccgt agtagccacc catggagccg ccgccacgcc
     9961 cggactcgcc gccgccgtag ccgccgccgg
//
LOCUS       DASPJI010000693        10775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_849964, whole genome shotgun sequence.
ACCESSION   DASPJI010000693 DASPJI010000000
VERSION     DASPJI010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10775)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10775
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849964"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VEL74_19600"
     CDS             <1..400
                     /locus_tag="VEL74_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG64795.1"
                     /translation="NARAAVLLTGELARRLQARGLALGRVVNISTDSARSFPGQISYG
                     ASKAALEAYTRSLAVELGPLGITVNCIAPGPVQTGWIGPELEAQVLPSIPLGRVGTPE
                     DIAAAAVFLASEQARWITGQVLQVAGGHAL"
     gene            complement(686..2671)
                     /locus_tag="VEL74_19605"
     CDS             complement(686..2671)
                     /locus_tag="VEL74_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HYG64796.1"
                     /translation="MIQTSLRARVLLAAIPALCLGLSAAEAAPRVTRLTPPSNLFSSG
                     DPNPPIISRFLPGQRFDLQATLAPDAGQTITRATFTIDGAPANGNVSLRPATVSGAPA
                     GATVATLRAYSNARPGVHLLRVTATQSDGATVSAQGNFEIAGIRGNGNAGGAKNVVIF
                     IGDGMGIAHRTAARIMLRGVRQGKANGALAMDTFPYTGLVMTHSLNSIVTDSSPGAAC
                     YSTGNKGNNNQHGVFPDDTTDNFDNPRVELIGEYLHRTRGKSLGIVTTSDVFDATPGA
                     FASHTQSRGAGTGIVDQYFDDRDLTGLTVLMGGGRKWFLPATVAGSARANSSDYVLPG
                     DLAAAWKAEPGALDPNRDLIAEFQQAGFAYAPDATTLTNMNTPRKLLGLFALSNMNVA
                     LDKVNGRRGASAVVADYGFPDQPMLEEMTEKALEVLSQDDDGFVLMVEGASIDKQAHN
                     MDSERWVLDTIELDHAIQKGLDFARRNPDTLLVVTADHECAGVNIIGGSMVPHGELQS
                     RSSANGGAQQLRSGVVGTYEAAGFPVYPIAGDGYPATTNVDRRMLIGYAGNADRNEDW
                     MTNPQPLRDSQQPFNGQAPLSNYPGGPTNRDTAGGFLVTGQVPDSSAVHTASDIPVSA
                     YGRGASLFTGVMDNTDVFFKIMQAVLGNPGRGNGGQR"
     gene            complement(2668..3180)
                     /locus_tag="VEL74_19610"
     CDS             complement(2668..3180)
                     /locus_tag="VEL74_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64797.1"
                     /translation="MSISKSLCLLLALVLVSALGASGGWAQPGDVPELRFDEIFASPV
                     GPGGLEYSAKARGLAGRRVRVAGYMVRRDAAPPGTFLLTPVPVQLHEAEYGLADDLPA
                     ATLLVEAPAWRGRPMPHEPGPLMVTGVLQLGNREEPDGRVSSVRLVLDGPDTPSDPTL
                     RSTEPKEGTP"
     gene            complement(3284..4447)
                     /locus_tag="VEL74_19615"
     CDS             complement(3284..4447)
                     /locus_tag="VEL74_19615"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HYG64798.1"
                     /translation="MAKRVGILTGGGDVPGLNAAIKGFVSRMEGEGYEVVGLRRGWAA
                     LLHIVPYTDADNDDWIVPLNRISTRVIDRSGGTILHSSRINPALTKPDGVPGHLRGKA
                     FPKRDDGRLDLTSAALEVLEFLRLDTLVAIGGDGTLSFARRLHLEGVKVVGVPKTMDN
                     DVYGTDYCIGFSTAVTRSVNFINDLRTVAGSHERYLVVELFGRNSGETCLLASYLSGA
                     DRALIAEVPFDTEAVVRMMEDDKRSNPSNYAVAAVSEGAFPVGGQPFESGEADAVGNR
                     ELGGVGRYLREALQKNTGDRVIYQRLGYLMRSGPPDSLDRLVANNFANLAADLILARK
                     AGQLVCIESGRYSSKPIEIVGEGVKRVDVERFYDSERYRPTIVGVMGLPMFLC"
     gene            complement(4455..5459)
                     /gene="gap"
                     /locus_tag="VEL74_19620"
     CDS             complement(4455..5459)
                     /gene="gap"
                     /locus_tag="VEL74_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003330786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HYG64799.1"
                     /translation="MATKVGINGFGRIGRSVFRILSDRSDVEVVAINDLYENEQLAYL
                     LKYDTLMREFPKEVTTDADHLYVDGRKIVMTAERDPTKIPWGDLGVDVVIESTGVHTK
                     KEALAKHLQAGAKKVILTVPAKDEIDATIVMGVNDDMLKPEHKLVSNASCTTNCLAPI
                     AKILDDRFGIDEGFITTVHAYTNDQRLADVPHKDFRRSRAAAENIIPTTTGAARAVGK
                     VLPQLKGKLDGMAMRVPVPDGSIVDLVCKLRANPSAEEVNAAVREAAEGPLKDIVEYS
                     EVPLVSSDIIGNPHSSIFDALSTKAAGDGYAKVVAWYDNEWGYSQRVVDLLDRLVEVG
                     "
     gene            complement(5460..6494)
                     /locus_tag="VEL74_19625"
     CDS             complement(5460..6494)
                     /locus_tag="VEL74_19625"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HYG64800.1"
                     /translation="MPSNIGLMGLGRIGRNIFRILYNSDDIRIQAISDVADHAALAYL
                     LRFDTILGKFPDEVSVRDGHLYAAGRQIKMLADETPGNIPWGDLGVDTVIEATARFRT
                     RAELEKHLEMGAKRVIVCTRTTEPPDITVVMGVNDGDLRPEHRIVSNASNTAHAAAPV
                     LHILHDAFGIHRGFLTTVHAYTNQQRLADVPAEDPRSGRAAAENIIPQETNSAEVVME
                     LLPELKGKLTGHAVNVPVANGSVADLVCWHDRPITAESVNEVVRTAAASQRWRGILEY
                     EDDPIVSSDILHDKSSGIFDSLATMVLGEKVSKTLCWFNNGWGYAHRVVDLLRRFEEI
                     DQKAGAREVK"
     gene            6848..8329
                     /locus_tag="VEL74_19630"
     CDS             6848..8329
                     /locus_tag="VEL74_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HYG64801.1"
                     /translation="MAGDPAATPETTGSPAAARVSVTPHERWDDAILYFVILDRFADG
                     DPRNNANVDRRAKGTFHGGDLAGLTQQLDEIADLGATAIWITPVVDNMDGFVTGAGFP
                     DWAYHGYWADDFTRVDPRFGTEAELKRLVDEAHARGIEVLLDVVYNHAGYESAYLKNP
                     ATRSWLRIESQGSCGKDDITTCVSGLPDFRTEEPQVADHLLEAHISMAERVGLDGFRL
                     DTVKHVTHGFWQEHRRRVNQRLGEDFFLIGEVWGGDAKSLDPWFAGDEMDAGFDFSFQ
                     GNVISFLQGRGRTVAFDRYLLSRDKVREGYLLSPYLSSHDVPGALMQLEGNTELFRLA
                     ALLQLTASGLPTIYYGEEVARPGGDWPDNRSDMPWGDRKVLPGAGLPRDEKLRADYKK
                     LIAIRRAHKALSRGDHQSVSSEGDLLVFARLYTGEGAESGDAVVVAVNRGAVPATASF
                     DAPAEWGDGPVRDLWNGSDVTRTGGKIEITVAPREARILAVQR"
     gene            8352..9446
                     /gene="ugpC"
                     /locus_tag="VEL74_19635"
     CDS             8352..9446
                     /gene="ugpC"
                     /locus_tag="VEL74_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HYG64802.1"
                     /translation="MADVRLIDIKKSYGPVNVIEKLNLEIRDEEFMVLVGPSGCGKST
                     ALRMIAGLEEITGGTIRIGERVVNDVPPKDRDIAMVFQSYALYPHMTVRENLEFGLKI
                     RKTPREEMNRRVDEAAQILGITEYLDRKPKQLSGGQRQRVAVGRAIVRKPAVFLFDEP
                     LSNLDAKLRVQMRAEISKLQKTLKTTTVYVTHDQIEAMTMGHRIAVMRAGKLQQVGTP
                     LEVYEQPANLFVAGFIGTPPMNFIPATLTDSGSTVTASGFKMPVPAALRGAIAGRDGL
                     KVVAGLRPENIREREREGGGPTVRLRARVEFVEPLGHEVIVHGRVGDDLLVAKADPHH
                     APEMGSEIDLVAEVEALHLFDAATEQRLGA"
     gene            9472..10680
                     /locus_tag="VEL74_19640"
     CDS             9472..10680
                     /locus_tag="VEL74_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018690633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HYG64803.1"
                     /translation="MIRTFRGILCPLFLLLAFPVLAAAQKEIVVWHGYRGGEKTAFEK
                     VVDQFNKSGKGVKVTTLAVPYDAFADKITAAVPRGKGPDVFIFAQDRLGGWVAAGNTV
                     EPLDFFLDAKIKDRYLKATLDAMTYQGNVYGLPLNFKALTLIYNKKLVQTPPKTTGQL
                     VAQAKKLTDPKTGRFGLAYWYSNFYYHSPLMNGFGGGVFQGRKPTLNSPANVKSLDYL
                     SGWMKQGFMPEEPSTALVTSLFNQGKAAMVFSGPWFLGEVAKGIDVGLAPLPTIDEAG
                     GKPMRPWMTVEGVYIAAPSKNKDAAFEFLKFVTDVPAAKVLALEGRQTPANKAVYADK
                     QVAADPVLKAFRTQVNNAVPMPNLPEMTMVWTPATTAMDTTVRKAATPKQALDVAQKE
                     VVELISKLKG"
BASE COUNT         1638 a         3716 c         3774 g         1647 t
ORIGIN      
        1 caacgcgcgg gccgccgtcc tcctgaccgg agagctggcc cgccgcctcc aggcccgggg
       61 gctcgccttg gggagagtgg tcaacatcag caccgattcg gcgcggagct tccccggcca
      121 gatcagctac ggggcgagca aggcggcgct cgaggcctac acccgcagcc tcgccgtgga
      181 gctcggaccc ctggggatca ccgtcaactg catcgcgccc ggccccgtcc agaccggctg
      241 gatcggaccg gagctggaag cgcaggtgct cccctcgatc cccctgggcc gggtcggcac
      301 ccccgaggac atcgccgccg ccgccgtctt cctcgcctcc gagcaggcgc gctggatcac
      361 cggccaagtc ctccaggtgg ccggcggcca cgccctctga acgcaatgcg cgccgccgga
      421 gactacgacg agctgtcgga ctttccgaca ggccgccgga atctccggcg agccggcgca
      481 accttcgaca gcccgttgga accttccaac gcgccgccgg agactacgac gagccgtcgg
      541 actttccgac aggccatcgg aatctccggc gagccatcgc aaccttcgac agcccgtcgg
      601 aaccttccaa cgcgccgccg gagactacga caaggaatcg gccccttccc ccgaccgcgg
      661 ggaaggggct tatcgcgtgg ccgggctacc tctgcccgcc gttgccgcgg ccggggttgc
      721 cgaggacggc ctgcatgatc ttgaagaaga cgtcggtgtt gtccatgacg ccggtgaaga
      781 gggaggcgcc gcgcccgtag gccgagaccg ggatgtctga ggcggtgtgc accgccgagg
      841 agtcggggac ctggccggtc accaggaagc cgccggcggt gtcgcggttg gtcgggccgc
      901 ccggatagtt gctgagcggc gcctggccgt tgaacggctg ttggctgtcg cgcagcggct
      961 gcgggttggt catccagtcc tcgttgcggt cggcgttgcc ggcgtagccg atgagcatcc
     1021 ggcggtcgac gttggtggtg gccggatagc cgtccccggc gatgggatag accgggaagc
     1081 cggccgcctc gtaggtgccc accacgccgc tgcggagctg ctgggcgccg ccgttcgccg
     1141 acgaccggct ctgcagctcg ccgtgcggca ccatcgagcc gccgatgatg ttgacgccgg
     1201 cgcactcgtg atccgccgtc accaccagca gcgtgtcggg gttgcggcgg gcgaagtcga
     1261 gccccttctg gatggcgtgg tcgagctcga tggtgtcgag gacccaccgc tcgctgtcca
     1321 tgttgtgcgc ctgcttgtcg atggacgccc cttcgaccat caggacgaag ccgtcgtcgt
     1381 cctggctcag cacctccagc gccttctccg tcatctcctc cagcatgggc tggtcgggga
     1441 agccgtaatc ggcgaccacg gccgaggcgc cgcgccggcc gttgaccttg tccagggcga
     1501 cgttcatgtt ggagagggcg aagaggccga ggagcttccg cggcgtgttc atgttggtga
     1561 gcgtggtggc gtccggagcg taggcgaagc cggcctgctg gaactcggcg atcaggtcgc
     1621 ggttggggtc gagggccccc ggctccgcct tccacgccgc cgccaggtcg ccggggagca
     1681 cgtagtcgct ggaattggcg cgggccgagc ccgccacggt ggcggggagg aaccacttgc
     1741 ggccgcctcc catgagcacg gtcaggccgg tgaggtcgcg gtcgtcgaag tactggtcca
     1801 cgatgccggt gccggcgccg cggctctggg tgtgcgaggc gaaggcgccg ggggtggcgt
     1861 cgaagacgtc cgaggtggtg acgatgccca gcgattttcc ccgggtgcgg tgcaggtact
     1921 cgccgatcag ctccacccgc gggttgtcga agttgtccgt ggtgtcgtcg gggaagacgc
     1981 cgtgctggtt gttgttgccc ttgttccccg tggagtagca ggcggcgccc ggcgaggagt
     2041 cggtgacgat ggagttgagg gaatgggtca tcaccaggcc ggtgtagggg aaggtgtcca
     2101 tggccagggc gccgttggcc ttgccctgcc gcacgccgcg gagcatgatg cgcgccgccg
     2161 tgcggtgggc gatccccatg ccgtcgccga tgaagatgac cacgttcttg gcgccgccgg
     2221 cgttgccgtt gccgcggatg ccggcgatct cgaagttgcc ctgggcgctg accgtggcgc
     2281 cgtcgctctg ggtcgcggtc actcgcagga ggtgcacgcc ggggcgggcg ttggagtagg
     2341 cgcgcagcgt cgcgacggtg gcgccggccg gggcgccgct gacggtggcc ggccggagag
     2401 agacattgcc gttcgccggc gcgccgtcga tagtaaaggt ggcgcgggtg atggtctgcc
     2461 cggcgtcggg ggcgagggtg gcctgcaggt cgaaacgctg gccgggcagg aagcgcgaga
     2521 tgatcggcgg gttgggatcg ccggagctga acaggttgct cggcggggtg agacgggtca
     2581 cccgcggcgc cgcctcggcg gcggagaggc ctagacagag cgccgggatg gcggcgagca
     2641 ggacccgcgc gcgcagagag gtctggatca aggtgtgccc tccttgggtt ccgtggaacg
     2701 aagcgttgga tcggacgggg tatcgggacc atccagcacc agccggacgg aggagacgcg
     2761 gccatcgggc tcttcgcggt tgccgagctg gaggacgccg gtgaccatga gcggcccggg
     2821 ctcgtgcggc atcggccggc cgcgccaggc cggcgcctcg acgagcagcg tggcggccgg
     2881 caggtcgtcg gcgaggccgt actcggcctc atggagctgg acggggaccg gggtcaggag
     2941 gaacgtgccg ggcggcgcgg cgtcgcgccg caccatgtag cccgcgacgc ggacccgccg
     3001 ccccgcgagc ccgcgggcct tcgccgagta ctcgagcccg ccggggccga ccggggacgc
     3061 gaagatctcg tcgaaacgca gctcggggac atccccgggc tgggcccacc ccccggaggc
     3121 gcccagcgcc gacaccagga cgagggcgag gagaagacag aggctcttgc tgatggacat
     3181 ggagaaccct cggccgggag gagacccggt gattcgggcc ctccggacga cgggggcagc
     3241 ctagaaaacg ggagtgacgg cggagcgtcg gcggcgcgac cgctcagcaa aggaacatcg
     3301 gcaatcccat gacgccgacg atggtgggac ggtaacgctc gctgtcgtag aagcgctcga
     3361 cgtcgacgcg cttgaccccc tcgcccacga tctcgatcgg cttggagctg tagcgcccgc
     3421 tctcgatgca gacgagctgg ccggccttgc gggcgaggat caggtcggcg gcgaggttgg
     3481 cgaagttgtt ggccaccagg cggtccaggg agtccggcgg cccggagcgc atgaggtagc
     3541 ccagacgctg atagatcacc cggtcgccgg tgttcttctg cagcgcctcc cgcaggtagc
     3601 gcccgacgcc cccgagctcg cggttgccga cggcgtccgc ctcgcccgac tcgaaaggct
     3661 ggccgccgac cgggaaggcc ccctcggaga ccgcggcgac cgcgtagttg ctggggttgc
     3721 tccgcttgtc gtcctccatc atgcggacca cggcctcggt gtcgaagggg acctcggcga
     3781 tcagcgcgcg gtccgcgccc gagaggtagg aggcgagcag gcaggtttcc cccgagttgc
     3841 ggccgaagag ctccaccacc agatagcgct cgtgggagcc cgccaccgtc cgcaggtcgt
     3901 tgatgaagtt gaccgaacgg gtgacggcgg tcgaaaagcc gatgcagtag tcggtgccgt
     3961 agacgtcgtt gtccatggtc ttcggcacgc cgacgacctt gaccccctcc aggtggaggc
     4021 ggcgggcgaa ggagagggtg ccgtcaccgc cgatcgccac cagggtgtcg aggcggagga
     4081 actcgagcac ctcgagggcg gccgaggtga ggtcgagccg cccgtcgtcg cgcttcggga
     4141 aggccttgcc gcgcaggtgc ccggggacgc cgtccggctt ggtgagcgcc gggttgatgc
     4201 gcgagctgtg gaggatcgtg ccgccgctgc ggtcgatgac gcgggtgctg atgcggttga
     4261 gcggcacgat ccagtcgtcg ttgtcggcgt cggtgtaggg gacgatgtgg agcagggccg
     4321 cccagcctcg gcgcagcccc accacctcgt acccctcccc ctccatccgc gacacgaacc
     4381 ccttgatcgc cgcgttcaac cccggcacgt cgccgccgcc ggtgaggatg ccgacccgct
     4441 tcgccatgcc cggcctaccc cacctccacc agccggtcga gcaggtccac cacgcgctgc
     4501 gagtagcccc actcgttgtc gtaccaggcc accaccttgg cgtagccgtc gccggccgcc
     4561 ttggtggaga gggcgtcgaa gatcgacgag tgcgggttgc cgatgatatc cgacgagacc
     4621 agcggcacct cgctgtactc gacgatgtcc ttgagcggcc cctcggccgc ctcgcgcacc
     4681 gcggcgttga cctcctcggc cgacgggttc gcccgcagct tgcagaccag gtcgacgatc
     4741 gagccgtccg gcaccggcac gcgcatcgcc atgccgtcga gcttgccctt gagctgcggc
     4801 agcaccttgc ccaccgcccg cgccgcgccc gtcgtcgtcg ggatgatgtt ctccgccgcc
     4861 gcccggctgc gccggaagtc cttgtgcggg acgtccgcca gccgctggtc gttggtgtag
     4921 gcgtgcaccg tggtgatgaa cccctcgtcg atgccgaagc ggtcgtcgag gatcttggcg
     4981 atcggcgcca ggcagttggt ggtgcaggag gcgttcgaca ccagcttgtg ctcgggcttg
     5041 agcatgtcgt cgttgacccc catcacgatg gtggcgtcga tctcgtcctt ggccggcacg
     5101 gtcaggatca ccttcttcgc acccgcctgg aggtgcttgg ccagcgcctc cttcttggtg
     5161 tgcacgccgg tcgactcgat gaccacgtcc acccccaggt ccccccaggg gatcttcgtc
     5221 gggtcgcgct ccgcggtcat gacgatcttc ctgccgtcga cgtagaggtg gtcggcgtcg
     5281 gtcgtcacct ccttggggaa ctcccgcatc agcgtgtcgt acttgaggag gtaggcgagc
     5341 tgctcgttct cgtacaggtc gttgatcgcc acgacctcga cgtcggagcg gtcggagagg
     5401 atccggaaga cgctgcggcc gatgcggccg aagccgttga ttcccacttt ggtcgccatt
     5461 tacttcacct cccgggcgcc ggccttctga tcgatctctt cgaagcgccg gagcaggtcc
     5521 acgactcggt gcgcgtagcc ccagccgttg ttgaaccagc agagggtctt cgacaccttc
     5581 tctcccagca ccatggtggc caggctgtcg aagatccccg agctcttgtc atgcaggatg
     5641 tcggacgaga cgatggggtc gtcctcgtac tcgaggatcc cccgccagcg ctgcgacgcc
     5701 gccgccgtgc ggaccacctc gttgaccgac tcggcggtga tcggcctgtc gtgccagcag
     5761 accaggtccg ccaccgagcc gttggccacc ggcacgttga ccgcgtggcc ggtgagcttg
     5821 cccttgagct ccggcagcag ctccatgacc acctcggccg agttggtctc ctgcgggatg
     5881 atgttctccg ccgccgcccg gccgctgcgc gggtcctcgg ccggcacgtc cgccagccgc
     5941 tgctggttgg tgtaggcgtg gacggtggtc aggaaccccc ggtggatgcc gaaggcgtcg
     6001 tgcaggatgt ggagcaccgg cgccgccgcg tgcgcggtgt tcgaggcgtt ggagacgatc
     6061 cggtgctcgg gccggaggtc gccgtcgttg acccccatga ccacggtgat gtccggcggc
     6121 tcggtggtgc gggtgcagac gatgacccgc ttcgccccca tctcgaggtg cttctccagc
     6181 tccgcccggg tgcggaagcg cgccgtcgcc tcgatcaccg tgtccacccc gaggtccccc
     6241 caggggatgt tgcccggcgt ctcgtcggcc agcatcttga tctggcgccc ggcggcgtag
     6301 aggtggccgt cgcgcacgct cacctcgtcc gggaacttgc ccaggatcgt gtcgaaacgc
     6361 aacaggtagg ccagggcggc gtgatcggcc acgtcgctga tcgcctggat ccggatgtcg
     6421 tcactgttgt aaaggatgcg gaagatgttc ctcccgatcc gccccaaccc catgagcccg
     6481 atgttactgg gcatcagcag cctccttccc ggcccctcgc cggccgtcgc aatattcgcg
     6541 ggccctcccc gaccgcggcg gcccaggtca gaccgtaaca cgttccgtgc cacggagggg
     6601 ccgcggccgg ccgccacggg gcggtcaccc gtcattgaca ggggtgtcac ccgatcccta
     6661 caatcggcca tcctgacagg ggccgtgtat ctgaaccctg gggggaggtc caatgaggtc
     6721 tcgcgtgccc agtccgggcg gagtcatctc ccgagcggcg cagcgggcct gctccgtttc
     6781 aaccctcgta acctgcctga tcctgggagc gtgtggcggt gcggtgcagg atccccaggc
     6841 gccgcccatg gccggcgatc cggccgccac gcctgagaca accggcagcc cggcggcggc
     6901 ccgcgtctcc gtcacgccgc acgagcggtg ggacgacgcc atcctctact tcgtcatcct
     6961 cgaccgcttc gccgacggcg acccgcgcaa caacgccaac gtcgaccgcc gcgccaaggg
     7021 caccttccac ggcggcgacc tcgccggcct cacccagcag ctcgacgaga tcgccgacct
     7081 gggcgccacc gcgatctgga tcacgccggt ggtcgacaac atggacggct tcgtcaccgg
     7141 cgccggcttc cccgactggg cctaccacgg ctactgggcc gacgacttca ccagggtcga
     7201 cccccgcttc ggcaccgagg cggagctgaa gcggctggtg gacgaggcgc acgcccgggg
     7261 gatcgaggtg ctcctcgacg tggtctacaa ccacgccggc tacgagtccg cctatctgaa
     7321 gaaccccgcc acccggagct ggctgcgcat cgagtcgcag ggctcctgcg gcaaggacga
     7381 catcacgacc tgcgtctccg gcctgcccga tttccggacc gaggagccgc aggtcgccga
     7441 ccatctgctg gaggcccata tctccatggc cgagcgggtg gggctcgacg gtttccggct
     7501 ggacacggtc aagcacgtga cgcacgggtt ctggcaggag caccggcgcc gggtgaacca
     7561 gcgcctggga gaggatttct tcctgatcgg cgaggtctgg gggggcgacg cgaaatcgct
     7621 cgacccctgg ttcgccggcg acgagatgga cgccggcttc gacttctcct tccagggcaa
     7681 cgtcatctcg ttcctccagg ggcgcggccg gaccgtggcc ttcgaccgct acctgctgtc
     7741 gcgcgacaag gtgcgggagg ggtacctcct ctccccctac ctctcgtcgc acgacgtgcc
     7801 cggcgcgctg atgcagctcg agggcaacac ggagctcttc cggctggcgg cgctcctcca
     7861 gctcaccgcc tcgggcctgc cgaccatcta ctacggcgag gaggtggcgc ggcccggcgg
     7921 cgactggccg gacaaccgca gcgacatgcc gtggggggac cgcaaggtcc tgccgggcgc
     7981 ggggctcccc cgcgacgaga agctgcgcgc cgactacaag aagctgatcg ccatccggcg
     8041 cgcccacaag gcgctctccc gcggcgacca ccagagcgtc tcctccgagg gggatctgct
     8101 ggtcttcgcc cgcctctata ccggagaagg ggccgaatcc ggggacgccg tggtggtggc
     8161 ggtcaaccgc ggcgcggtgc cggccacggc gagcttcgac gctcccgcgg agtgggggga
     8221 cgggccggtg cgcgacctgt ggaacgggag cgacgtcacc cgcaccgggg gcaagatcga
     8281 gatcaccgtg gcgccccgcg aggcgcggat cctcgccgtg cagcgctaag gagcggcgat
     8341 aaggagcgcg aatggcagac gtcaggctca tcgacatcaa gaagagctac ggcccggtca
     8401 acgtcatcga gaagctgaac ctcgagatcc gggacgagga gttcatggtc ctggtcgggc
     8461 cgtccggctg cggcaagtcc acggcgctgc gcatgatcgc cggcctcgag gagatcaccg
     8521 gcggcaccat ccggatcggc gagcgggtgg tcaacgacgt gccgccgaag gaccgcgaca
     8581 tcgccatggt cttccagagc tacgcgctct atccccacat gaccgtgcgc gagaacctgg
     8641 agttcggcct gaagatccgc aagacgccgc gcgaggagat gaaccggcga gtggacgagg
     8701 cggcgcagat cctcggcatc accgagtacc tggaccgcaa gccgaagcag ctctccggcg
     8761 gccagcgcca gcgcgtcgcc gtcggccgcg ccatcgtccg caagcccgcc gtcttcctgt
     8821 tcgacgagcc gctgtcgaac ctcgacgcca agctgcgcgt ccagatgcgg gccgagatct
     8881 cgaagctcca gaagaccctg aagaccacca ccgtctacgt cacccacgac cagatcgagg
     8941 cgatgaccat ggggcatcgg atcgcggtca tgcgcgccgg caagctgcag caggtgggca
     9001 cgccgctgga ggtctacgag cagccggcca acctgttcgt ggcgggcttc atcggcacgc
     9061 ccccgatgaa cttcatcccc gccacgctga ccgacagcgg gtccaccgtc accgcctccg
     9121 gcttcaagat gccggtgccc gcggcgctgc gcggcgccat cgccggccgc gacgggctga
     9181 aggtggtggc cggcctgcgc cccgagaaca tccgcgagcg cgagcgggag gggggcggcc
     9241 cgacggtgcg gctgcgcgcc cgggtggagt tcgtcgagcc gctgggccac gaggtgatcg
     9301 tccacggccg cgtcggcgac gacctgctgg tggccaaggc cgatccgcac cacgcgccgg
     9361 agatggggtc ggagatcgac ctcgttgccg aggtggaggc gctgcacctc ttcgatgccg
     9421 ccacggagca gcggctcggg gcctgaaacc gatcgtcaaa ggagaacagc catgatcaga
     9481 actttccgcg gcatcctctg cccgctcttc ctgctcctcg cgtttccagt cctcgctgca
     9541 gcccagaagg agatcgtcgt ctggcacggc taccgcggcg gcgagaagac ggctttcgaa
     9601 aaggtggtcg atcagttcaa caagtccggc aagggggtca aggtgacgac cctcgccgtc
     9661 ccctacgacg ccttcgccga caagatcacg gcggcggtgc cgcgcggcaa agggccggac
     9721 gtcttcatct ttgcccagga ccgcctcggc ggctgggtcg ccgccggcaa cacggtcgag
     9781 ccgctcgatt tcttcctcga cgccaagatc aaggaccgct acctcaaggc gacgctggac
     9841 gcgatgacct atcagggcaa cgtctacggc ctgccgctca acttcaaggc gctcaccctg
     9901 atctacaaca agaagctggt gcagacgccg cccaagacca cagggcagct cgtggcgcag
     9961 gcgaaaaaac tgaccgatcc caagaccggc cgcttcggcc tcgcctactg gtactccaac
    10021 ttctattacc actcgccgct gatgaacggt ttcggcggcg gcgtcttcca gggccgcaag
    10081 ccgacgctca actcgccggc caacgtcaag tcgctcgact atctcagcgg ctggatgaag
    10141 cagggtttca tgcccgagga gccgtcgacc gcgctcgtca cctcgctctt caatcagggc
    10201 aaggcggcca tggtgttcag cggcccctgg ttcctcggcg aggtcgccaa ggggatcgac
    10261 gtcgggctcg cgccgctgcc caccatcgac gaggccggcg gcaagccgat gcgcccatgg
    10321 atgaccgtcg agggggtcta catcgctgcg ccgtcgaaga acaaggacgc cgccttcgag
    10381 ttcctcaagt tcgtgaccga cgtgcccgcg gccaaggtcc tggccctcga agggcgccag
    10441 acgccggcca acaaggcggt ctacgcggac aagcaggtcg ccgccgaccc cgtcctcaag
    10501 gcgttccgga cgcaggtgaa caacgcggtg ccgatgccca acctccccga gatgaccatg
    10561 gtctggacgc ccgcgaccac tgccatggac accacggtcc gcaaggcggc gacgccgaag
    10621 caggcgctgg acgtcgccca gaaggaagtg gtggagctga tctcgaagct caagggatga
    10681 ccccatgacc gccgacccgc agccccccac cccgccgtcg caccggcgcc gcttcctgat
    10741 cggcgtcgtg gtggccgcgc tcctcgccgc cggcc
//
LOCUS       DASPJI010000694         4655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_342273, whole genome shotgun sequence.
ACCESSION   DASPJI010000694 DASPJI010000000
VERSION     DASPJI010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342273"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..>4655
                     /locus_tag="VEL74_19645"
     CDS             56..>4655
                     /locus_tag="VEL74_19645"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64804.1"
                     /translation="MAHCLTWAHAYGMTAEDRVLQFASASFDASVEQIFAALLSGATL
                     VMRGPELWGTRELPRRITELGLTLADLPTAFWNRWVRDLEDVEDVPGRLRLVGIGGEE
                     LLAESARLWNRSPLARVRLLNSYGPTEAVVSATLYEVPPGDGESGPVPIGRPLPGRVA
                     RVLDRSGQPQPMGIPGELCVGGPLARGYLGRPDLTAAAFVPDLYAETPGARLYRTGDL
                     VRRRPDGQIEFLGRTDDQVKVRGFRIEPGEIEAALAEHPEVREAAVLAVPGPGGERRL
                     VGFVAPERPADLTVFLRQRLPDYMVPAAWAMLPSLPINASGKVDRAALRRLAVEVEAG
                     AEEGGASPRTPEEELLAGIFAELLGRERVGAHDDFFALGGHSLLATRLVSRISRVFGV
                     DLPVSAVFQEPTVVRLAARIAAGRAETAPPIRPVPRRPGEPLPLSFAQRRLWFLDQLE
                     PGSAIYNVPGSVCLVGLLDVPALVVAVAEIARRHEILRTVFPVQAGEPAQIVQPVVPA
                     LPVIDLAGLPEAARKAEAERRVRQEAARPFDLARGPVCRVALVRIATEEHLLLVTLHH
                     IASDGWSLGLFLDELTALYGGAALPALPVQYADYAVWQAERLRGEGLERQLSWWRDRL
                     AGLPVLELPADHPRPSTRDPRGASRSLELPAELGEAVARLARSRGVTLFMALLGAFQA
                     LLARYTGEGVIPVGSPVAHRDRPEIEPLIGLFVNTLVLTARVDDDPSVAELLARVREA
                     SLGAYAHQEIPFERLVEELAPSRDLSRNPLFQVMFVLEEPLPARQAGGLLLEPVRSDS
                     GAAKFDLLLSVTPEPGGGLRVATEHAAALFEAPTMDRLLGHWAALLAGAAADPGRRLS
                     ELPLLGAAERAQILTEWNDSGRTWPEGLRLHDLVRAQAERTPEAEAVVGEAERITYRE
                     LTVRSSRIARLLRRLGVGPERRVGICLRRTPDMIAVILGVLEAGGAYVPLDPAYPVER
                     IELMYRDSGAGVLLTETAVADRVGFGAGIVLLDSVDFSVGEGVSPSPTLDSEDSDPRN
                     LAYVIYTSGSTGRPKGVPIEHRSAVSLVLWALELFPREDLAGVLAATSIAFDLSVFEI
                     FVPLSAGGKVILAGNVLELPQLAAIHEVTLVNGVPSPVAELVRGRLPVGLRTVNLAGE
                     ALPADLAERLYAHPQIQRVYNLYGPTEDTTYSTGSLVPRGPRGTTVPAIGRPLFNSRA
                     YVLGRHGEPVPIGVVGELYLGGDGLARGYLDRSELTAERFVPDSFGDGDRLYRTGDRA
                     RLRPDGEIEYLGRADFQVKISGIRIELGEIESTLAGHPAVRGAVVVARADGPGGGPRL
                     VAYVVGEAGHDELAAWLRERLPAAMVPSAWVEMPALPLTPNGKVDRKRLPAPDWGGRT
                     GETAAPRTPAEEKVAAAFREALGVERVGAFDSFFELGGHSLLAVRAAFRLAEAFGVEV
                     PVSTLFQAPMVAALAEKLTGVPARPDERVRAEGPGPFPLSLAQRRLWFLNQLEPGDSA
                     YHLWVPVRLGGELAVEPLRRSLAEIVRRHEPLRTVYGEV"
BASE COUNT          539 a         1592 c         1812 g          712 t
ORIGIN      
        1 tacatcaggg tccacgggtc ggcctaaggg cgtcggggtg agccatgggg cggcggtggc
       61 gcactgcctg acttgggcgc acgcgtacgg catgaccgcc gaggatcggg tgctgcagtt
      121 cgcctccgcg agcttcgacg cgtcggtcga gcagatcttc gcggccctcc tctcgggcgc
      181 gacgctggtg atgcgggggc cggagctgtg gggcacgcgg gagctgccgc ggcgcatcac
      241 cgagctgggc ctcacccttg ccgacctccc cacggcgttc tggaaccgtt gggtccgtga
      301 cctggaggat gtcgaggacg tccctgggcg gctccgcctg gtggggatcg gcggcgagga
      361 gctgctggcg gagagcgccc gcctctggaa ccggtctccg ctggcgcggg tgcggctcct
      421 caacagctac ggcccgaccg aggcggtggt ctccgcgacc ctttacgagg tcccgccggg
      481 agacggcgag tcagggccgg tgcccatcgg ccggccgctg ccgggccgcg tcgcccgggt
      541 gctggaccgt tccggccagc cacagccgat gggcatccct ggcgagctgt gcgtgggcgg
      601 ccccctggcg cggggctacc tgggccggcc ggacctcacg gccgcggcgt tcgtccccga
      661 tctctacgcg gagacacccg gcgcgcgtct ctaccgcacg ggggacctgg tgcggcgccg
      721 gcccgacggc cagatcgagt tcctggggcg gactgacgac caggtgaagg tgcgcgggtt
      781 ccgcatcgag cccggggaga tcgaggcggc gctggcggag caccccgagg tccgcgaagc
      841 cgccgtgctg gcggtgccgg gaccgggtgg ggagcggcgg ctggtcgggt tcgtggcgcc
      901 tgaaaggccc gcggacctca ccgtcttcct gcgccagcgc ctgcccgact acatggtccc
      961 ggccgcctgg gccatgctcc catccctccc gatcaacgcc agcggcaagg tggaccgggc
     1021 ggcgcttcgc cgcctggcgg tggaggtgga ggccggtgcg gaggaggggg gcgcttcccc
     1081 ccgcactccc gaggaggagt tgctggccgg gatcttcgcc gagctgctgg gacgggagcg
     1141 ggtgggggcc cacgacgact tcttcgcgct cggcggtcac tccctgctgg ctacgcggtt
     1201 ggtctcccgg atctcgcgcg tcttcggcgt cgacctgccg gtgagcgcgg tcttccagga
     1261 gcccacggtc gtccgcttgg cggcccggat cgccgccggc cgggcggaga cggcgccgcc
     1321 gatccggccg gtgccccgga ggcccggcga gccgctgccg ctctcctttg cccagcgccg
     1381 gctctggttc ctggaccagc tcgagccggg gagcgcgatc tacaacgtcc cgggttcggt
     1441 ctgcctcgtg ggcctcctgg acgtgccggc cctggtggtg gcggtggctg agatcgcgcg
     1501 gcgtcacgag atcctccgca ccgtgttccc tgtccaggcg ggtgagccgg cgcagatcgt
     1561 ccagcctgtg gttccagccc tgccggtgat cgacttggcc ggcctccctg aggcggcccg
     1621 gaaggccgag gcggagcggc gggtgcggca ggaagccgct cggcccttcg acctggcgcg
     1681 ggggccggtc tgccgggtcg cgctcgtccg gatcgccacg gaggagcacc tccttctggt
     1741 caccctccac cacatcgcct cggacggctg gtccctgggc ctcttcctgg acgagctgac
     1801 tgccctctac gggggagcgg cgctgccggc gctgccggtc cagtacgccg attacgcggt
     1861 ctggcaggcg gagcggctgc gcggcgaggg gctggagcgc cagctctcct ggtggcgcga
     1921 tcggctcgcg ggcctgccgg tgctggagct gcccgcggac cacccgcggc cgtccacgcg
     1981 ggacccgcgg ggcgcctccc gctcgctgga gctgccggct gagctggggg aggcggtggc
     2041 gcgcctcgcc cggagccggg gcgtcacgtt gttcatggct ctgctcggcg ccttccaggc
     2101 cctgctcgcc cgttacaccg gcgagggggt gatccccgtc ggctcgccgg tcgcccaccg
     2161 cgaccggccg gagatcgagc ccctcatcgg actcttcgtc aacacgctgg tgctgacggc
     2221 gcgggtggac gacgacccgt ccgtggcgga gctgctggcg cgggtgcggg aggcctccct
     2281 cggcgcctac gcccaccagg agatcccctt cgagcgtctg gtcgaggagc tggcgccgag
     2341 ccgtgacctg tcccggaacc cgctcttcca ggtgatgttc gtgctggagg agccgcttcc
     2401 cgcgcgtcaa gccggcggcc tgctgctgga gccggtgcgc agcgacagcg gcgcggcgaa
     2461 gttcgatctt ctcctctccg tgacgcccga gcctgggggc ggcctccggg tggccacgga
     2521 gcacgccgcc gccctcttcg aagcgccgac catggaccgc ctgctgggcc actgggcggc
     2581 gctgctcgcc ggggccgcgg ccgatcctgg ccgccggctg tcggagctgc cgctcctcgg
     2641 cgcggcggag cgggcgcaga tcctgacgga gtggaacgac agcgggcgga cctggccgga
     2701 agggctccgt ctccacgatc tggtgcgcgc ccaggcggag cgcacccccg aggccgaggc
     2761 ggtggtggga gaggcggagc ggatcaccta ccgggagctg acggtgcgtt ccagccgcat
     2821 cgcccggctc ttgcgcagac tgggcgtcgg gcccgagcgg cgggtgggga tctgcctgcg
     2881 gcgcacgccg gacatgatcg cggtgatcct cggggtgctg gaggctggcg gcgcctatgt
     2941 gccgctggac ccggcctatc ccgtggaacg gatcgagctg atgtaccgcg actcgggggc
     3001 aggggtgctg ctgaccgaga cggcggtggc ggaccgggtg ggttttgggg cggggatagt
     3061 gctgctggat tccgtcgatt tttctgtagg ggagggggta agcccctccc ctacactgga
     3121 ttcagaagat tcagacccgc gcaacctggc ctacgtcatc tatacatcgg gctcgacggg
     3181 gcggccgaag ggggttccca tcgagcaccg gagcgcggtc agcctggtgc tgtgggcgct
     3241 ggagctgttc ccgcgggaag acctcgcggg ggtgctggcg gcgacgtcca tcgctttcga
     3301 cctgtcggtc ttcgagatct tcgtgccgct gtccgccggc ggcaaggtga tcctggcggg
     3361 gaacgtcctg gagctgccac agctcgcggc catccacgag gtgacgctgg tcaacggcgt
     3421 tccttcgccg gtggccgagc tggtgcgggg acgcctcccc gtgggcctcc gcacggtgaa
     3481 cctggcaggc gaggcgctcc cggcggatct ggccgagagg ctctatgccc atccgcagat
     3541 ccagcgggtc tacaatcttt acggccctac cgaggacacc acctactcca ccgggtcgct
     3601 ggtaccccgg ggcccccgcg ggacaacggt gcccgccatc ggccggccgc tcttcaacag
     3661 ccgcgcctac gtgctgggac ggcacggcga gccggtgccc atcggtgtcg tcggcgagct
     3721 gtacctcggc ggcgacggtc tggccagggg gtatctcgac cggtcggagc tgacggcgga
     3781 gcggttcgta cccgattcct tcggagacgg tgaccgccta taccggaccg gtgaccgggc
     3841 gcggctccgg ccggacggcg agatcgagta cctggggagg gccgacttcc aagtcaagat
     3901 cagcggcatc cgcatcgagt tgggggagat cgagtcaacg ctagccgggc acccggcggt
     3961 gcggggagcc gtggtggtgg cgcgcgccga tgggccgggc ggcgggccgc ggctggtggc
     4021 ctatgtggtg ggggaggcgg gccacgacga gttggcggcg tggctgcggg aacggctgcc
     4081 ggcggcgatg gtgccgtccg cctgggtgga gatgccggcg ttgccgctca cccccaacgg
     4141 caaggtggac cgcaagcggc tgccggcgcc ggactggggc ggtcgcacgg gcgagacggc
     4201 ggcgccgcgc accccggccg aggagaaagt ggccgcggcc ttccgggagg cgctgggagt
     4261 ggagcgggtc ggggcgttcg actccttctt tgagctgggc ggccactcgc tgctggcggt
     4321 gcgcgctgcc ttccggctcg ccgaggcgtt cggcgtcgag gtgccggtct ccaccctttt
     4381 ccaggctccg atggtggcgg cgctcgccga gaagctgacc ggggtgccgg cgcgtccgga
     4441 cgagagggtc cgcgccgagg gaccgggacc gttcccgctg tccctggccc agcggcgcct
     4501 ctggttcctg aaccaactgg agccgggcga ttccgcctac cacctatggg tgccggtccg
     4561 cctggggggt gaactggcgg tggagccctt gcggaggagc ctcgccgaga tcgtccgccg
     4621 tcacgagccg ctgcgcacgg tctacggcga agtgg
//
LOCUS       DASPJI010000695         6089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_443825, whole genome shotgun sequence.
ACCESSION   DASPJI010000695 DASPJI010000000
VERSION     DASPJI010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443825"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..1509)
                     /gene="ftsZ"
                     /locus_tag="VEL74_19650"
     CDS             complement(313..1509)
                     /gene="ftsZ"
                     /locus_tag="VEL74_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018753246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HYG64805.1"
                     /translation="MTQEPIETAPARIKVVGVGGGGGNAVNRMIDAQLRGIEFITANT
                     DLQALNKCRAPIKLPIGNLLTKGLGAGADPEVGRKAALEDTERILETLEGADMVFLTA
                     GLGGGTGTGAAPIIASLAAEIGALTVAVVTKPFGFEGRRRMQQADRGIEELRSAVDTL
                     ITIPNERLLNFVERGTPLSEAFRIADDVLRQAVQGISDLITIPGEVNVDFADVRTIMT
                     GMGMALMGTGLAKGEHRALEAAQRAISSPLLEETSIQGAKGVLINISGGYDLTLHEVA
                     EAARIIADAVDPDANIISGMVIDATLEEAMKVTVIATGFDHSVSERPRMSAPPLSINR
                     PVAVAPSLAAATAIEREEPEKPMELPFYRKAIAHVRGEDPNGYGPNWSNVDDYDIPTV
                     LRKQMD"
     gene            complement(1592..2830)
                     /gene="ftsA"
                     /locus_tag="VEL74_19655"
     CDS             complement(1592..2830)
                     /gene="ftsA"
                     /locus_tag="VEL74_19655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HYG64806.1"
                     /translation="MAKPEPYFVGIDIGSSKVGVLIGQRDGKGGIEVVGKGLAPNRGT
                     RKGNIVNVEATVEALKQASEEAEVMAGVEISRAYVGVAGADIRSVNSRGMVTVARKDR
                     EITRQDIQRVLEAAQSAALPSDREILHAIPQEFIVDEQGGISEPLGMQGTRLEVMVHL
                     VTGNVTRSKTLLTCVNRAGIEVVEMVFEPLATAEAVLSADERELGVLLIDMGAGTTEY
                     ALFAEGEIQYSAVAPIGAGHFTNDLAMVLRTPFAEAERIKIKQGCCLPSMVTEEEGIA
                     VPTVAGGTSRVVPKRELCEILQPRAEELFNLMREDLLRHASDEGLRGGVVLTGGGAQL
                     DGLLELSEQIFDAGVRYGLPRGLGGLVDVISSPTWSTASGLLLYALHAEESQVKVQKK
                     AGFSVRGMVGSLRGMFSDLL"
     gene            complement(2845..3741)
                     /locus_tag="VEL74_19660"
     CDS             complement(2845..3741)
                     /locus_tag="VEL74_19660"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HYG64807.1"
                     /translation="MTGTLAGTPMGPVGTPGAGGRVLDFKRRSAPPRRRRPSFLRSLL
                     RPLTMALALVALPLGAGVWVLTSGRFDLRQVIVEGGEGQGQRVSAAWIERALAPYQGK
                     NLVLMPLSELVERLHRNPWIDTVEVEKELPDGLRVAFTERRPVALLLRQGALAYADAE
                     GRPIAPITSPQEADKARRDGLLMVSFSHPLLRSGGVSRAIAVAGELGRVEPDWAAHLS
                     RIEVLGEEDFRLYTGALPFPLLVRSGEVSDKAARLGKLLPELMTRYPEIEAVDLRFSR
                     RIVVQPAASPQSGNGVGPQAIF"
     gene            complement(3738..5147)
                     /gene="murC"
                     /locus_tag="VEL74_19665"
     CDS             complement(3738..5147)
                     /gene="murC"
                     /locus_tag="VEL74_19665"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HYG64808.1"
                     /translation="MFNQFTDLSHIHFVGVGGAGMSGIAEILRAYSLSNLVVSGCDQS
                     LSEATQRLIELGITVHQGHSPDHLDGVDLVVISSAVAEDNAEVREARRLGIPVVRRAE
                     MLGELMRLRYGIGIAGTHGKTTTTSLVGTVLTDAGLDPTVIVGGRLRLLGTGARLGQS
                     EYLVAEADEFDRSFLRLTPVIAAITTIDRDHLDTYGDMQAIEDAFVEFANRVPFFGRL
                     ILCLDDPNVQRILPRVAERRIITYGFSPQADLSAVDVEAHPKGSRFAVRLREGRLGRI
                     DLGRIELPMPGRHNVLNALAAVGVGLSLRIGFDAIARSLAGFSGVHRRFELLGTWHGA
                     AVVDDYAHHPTEVVATLQAARQTFPGGRVLAVFQPHLFSRTRDLADAFGSALLGADMA
                     LVTDVYPSRETPIPGVTGELVLEAARRSGHRDVHYVPAWKDAPEMLRQEVREGDVVVT
                     LGAGDVNRLAQGLAKEGTS"
     gene            complement(5147..>6089)
                     /locus_tag="VEL74_19670"
     CDS             complement(5147..>6089)
                     /locus_tag="VEL74_19670"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HYG64809.1"
                     /translation="PARGIPFHPLPARPLLGRGLFGKAQALTTLAGSAQAASALIRRL
                     GVDAVLGTGGYVSAPAVLGARFAGRPILLLEPNARAGVANRWLSRWATGAAVGYRDTI
                     GDLRCPCWVTGVPVRAAFFAVPPLSSSLTAPRLLVLGGSQGARQINQTVPAAVARLVQ
                     RLPELSVLHQAGEKNLEETRQAYADAGVGPGHVEVVPFLDDVAGAMAASHLLVSRAGA
                     ITVAEICAAGRPSVLLPLVIAKAHQVDNARLLAEEGAAEMRTPDQVTSEGLADLLAEL
                     LADGERLARMGRAARSLARPGAAAAVASRLEELGGEG"
BASE COUNT          887 a         2199 c         2129 g          874 t
ORIGIN      
        1 ctcttccgca aggcgacgcg ggtggagatc gagtccgacc cgcccatggc ctggaacgtc
       61 gacggcgagg tcatcggcga gggcccggcg cgcttcgagg tgctgccgcg ggcactggag
      121 gtggtggtgg ggcccgacga agaggaaccg cggaaagaac ccggatagaa gagaggggag
      181 gcgagacccc ggcctgctcc cgtccccctt ggaaatgggg agcgggcagg ccacgggcgc
      241 ccggacacgg cgcgcgggga gaggcctccc tgccgcctgc ggcgcgagga cgccggcgag
      301 cgacggggag gcctagtcca tctgcttcct cagcacggtc gggatgtcgt agtcgtcgac
      361 gttcgaccag ttgggcccgt aaccgttggg gtcctcgccg cggacgtggg cgatcgcctt
      421 gcgatagaac gggagctcca tcggcttctc gggctcctcg cgctcgatgg ccgtggccgc
      481 ggcgaggctc ggagcgaccg ccaccggacg gttgatcgac agcggcggcg ccgacatgcg
      541 cggccgttcg gagacgctgt ggtcgaagcc ggtggcgatg accgtcacct tcatcgcctc
      601 ctcgagcgtg gcgtcgatca ccatgccgga gatgatgttg gcgtccgggt cgacggcgtc
      661 ggcgatgatg cgcgccgcct cggcgacctc gtgcagcgtc aggtcgtagc cgccggagat
      721 attgatcagc acccccttgg cgccctggat ggaggtctcc tccaggagcg gcgacgagat
      781 cgcccgttgc gccgcctcga gcgcccggtg ctcgcccttg gcgaggccgg tgcccatgag
      841 cgccatgccc atgccggtca tgatggtgcg cacgtcggcg aagtcgacgt tgacctcgcc
      901 cgggatggtg atcaggtcgc tgatgccctg caccgcctgg cgcagcacgt catcggcgat
      961 gcggaacgcc tcggacagcg gcgtgccgcg ctcgacgaag ttgagcaggc gctcgttggg
     1021 aatggtgatc agcgtgtcca ccgcgctacg cagctcctcg atgccgcggt cggcctgctg
     1081 catgcggcgc cgcccctcga agccgaaggg tttggtgacc accgccacgg tgagggcgcc
     1141 gatctcggcc gccagcgagg cgatgatcgg cgccgcgccg gttccggtgc cgccgcccag
     1201 gccggcggtg aggaagacca tgtcggcccc ctccagcgtc tcgaggatgc gctcggtgtc
     1261 ctcgagcgcc gccttgcgcc cgacctccgg atcggcgccc gcccccaatc ccttggtgag
     1321 caggttgccg atcggcagct tgatcggcgc ccggcacttg ttgagcgcct gcaggtcggt
     1381 gttggcggtg atgaattcga tgccccggag ctgggcgtcg atcatgcggt tgacggcgtt
     1441 gccgccgccg ccgccgaccc ccaccacctt gatgcgcgcc ggcgccgtct cgatcggctc
     1501 ttgagtcacg gtcggcttct ccttcccgtt cggagagtcc tggtcgtcga aaagaatcat
     1561 gaggccaccc ccttctatgg gttcggtcga tttagagcaa gtcggagaac ataccgcgca
     1621 ggctgccgac catcccgcgc acgctgaaac cagccttctt ctgcaccttg acctggctct
     1681 cctcggcgtg cagggcgtag agcaggagcc ccgaggcggt ggaccaggtg ggactgctga
     1741 tgacgtcgac caggcccccg agaccgcggg gcaggccgta gcggacgccg gcgtcgaaga
     1801 tctgctctga cagctcgagc aggccatcga gctgcgcacc gccgccggtg agcaccacgc
     1861 cgccgcgcag cccctcgtcc gaggcgtggc gcagcaggtc ctcgcgcatg aggttgaaca
     1921 gctcctcggc gcgcggctgg aggatctcac acagctcgcg cttgggcacc acgcgggagg
     1981 tgccgccggc caccgtcggc acggcaatcc cctcctcctc ggtgaccatg ctgggcaggc
     2041 agcagccctg cttgatcttg atgcgctcgg cctcggcgaa gggggtgcgc agcaccattg
     2101 ccaggtcatt ggtgaagtgg ccggcgccga tcggcgccac ggcgctgtac tggatctccc
     2161 cctcggcgaa gagggcgtac tcggtggtcc cggcccccat gtcgatgagg agcaccccga
     2221 gctcgcgctc gtcggccgag agcaccgcct cggcggtggc gagcggctcg aagaccatct
     2281 ccaccacctc gataccggcg cggttgacgc aggtgagcag tgtcttcgag cgggtgacgt
     2341 tgccggtgac caggtggacc atcacctcca ggcgggtccc ctgcatcccc agcggctcgc
     2401 tgatcccccc ctgctcgtcg acgatgaact cctgcgggat ggcgtgcagg atctcccggt
     2461 ccgacggcag cgccgccgac tgggccgcct ccagtacgcg ctggatgtcc tggcgggtga
     2521 tctcgcggtc cttgcgggcc acggtcacca tgccgcgcga gttgacgctg cgaatgtcgg
     2581 cgcccgcgac gccgacatag gcgcgggaga tctccacccc cgccatgacc tcggcctcct
     2641 cgctcgcctg cttcagggct tccaccgtcg cctcgacgtt gacgatgttg cctttgcggg
     2701 tgccgcggtt gggtgccagg cccttgccga ccacctcgat gccccccttg ccgtcgcgct
     2761 ggccgatgag caccccaacc ttggatgacc cgatgtcgat ccccacgaag tacggttctg
     2821 gtttggccat ttgcctgacc ccctctagaa aatcgcctga ggcccgacgc cgttcccaga
     2881 ctgcggcgag gccgcgggct ggacgacgat ccggcgtgaa aaacggagat cgaccgcctc
     2941 gatctccgga tagcgcgtca tgagctccgg aaggagcttt ccgagccgtg ctgccttgtc
     3001 cgatacctcg ccgctccgga ccaggagcgg aaacgggagg gccccggtgt acagccggaa
     3061 atcctcctcc cccaatacct cgatgcgcga caggtgggcg gcccagtcgg gctccacccg
     3121 tcccagctcc cccgccaccg cgatggcgcg cgagacgccg ccggagcgca gcagcgggtg
     3181 cgagaagctc accataagga ggccgtctcg ccgcgccttg tccgcctcct gcggcgacgt
     3241 gatcggggcg atggggcgcc cctcggcgtc ggcgtaggcc agcgccccct ggcgcagcag
     3301 cagggccacc ggcctgcgct cggtgaacgc cacccgcaaa ccatccggca gctccttctc
     3361 cacctccacc gtgtcgatcc aggggttgcg atggaggcgc tcgaccaact cggacagtgg
     3421 catcagcacc aggtttttcc cctgataggg cgccagcgcc cgctcgatcc aggcggcgga
     3481 gacgcgctgc ccttgccctt cgccgccttc cacgatcacc tgccgcaggt cgaagcggcc
     3541 cgaggtcagc acccagactc cggcccccag cggcagggcg accagcgcca gggccatggt
     3601 gagcggccgc aacagcgacc ggaggaagct cggccgacgg cggcgcggcg gcgcgctgcg
     3661 gcgcttgaaa tcgagcaccc ggccgccggc acccggcgtc cccacggggc ccatcggcgt
     3721 tccggcgagg gtcccggtca cgacgtaccc tccttcgcca gaccctgggc cagccgattg
     3781 acatcgccgg cgccgagcgt caccaccacg tccccctcgc gcacctcctg gcgcagcatc
     3841 tccggcgcgt ccttccacgc cgggacgtag tggacatcgc ggtggccgct gcgccgcgcg
     3901 gcctcgagca ccagctcgcc ggtgacgccg gggatgggcg tctcgcgcga cgggtagacg
     3961 tcggtcacca gcgccatgtc ggcgcccagc agcgcgctgc cgaaggcgtc cgccagatcg
     4021 cgggtgcggg agaacaggtg cggctggaag accgccagca cccgcccacc ggggaaggtc
     4081 tggcgcgccg cctggagcgt cgccaccacc tcggtcgggt ggtgtgcgta gtcgtccacc
     4141 accgccgcgc cgtgccaggt gcccagcagc tcgaagcgcc ggtgcacccc ggagaagccg
     4201 gcgagggaac gggcgatggc gtcgaagccg atgcgtagcg acagcccgac gcccaccgcc
     4261 gccagggcat tcagcacgtt gtgccttccc ggcatcggca gctcgatccg cccgaggtcg
     4321 atgcggccga ggcgcccctc gcgcaggcgc acggcgaagc ggctcccctt ggggtgcgcc
     4381 tcgacgtcca ccgccgacag gtccgcctgc ggcgagaagc cgtaggtgat gatgcggcgc
     4441 tccgccaccc gcggcaggat gcgctgcacg ttcgggtcgt cgaggcagag gatcagccgg
     4501 ccgaagaacg gcacccggtt ggcgaattcg acgaaggcgt cctcgatcgc ctgcatgtcg
     4561 ccgtaggtat ccaggtggtc gcggtcgatg gtggtgatcg cggcgatgac cggcgtcagc
     4621 cgcaggaagc tgcggtcgaa ctcgtcggcc tcggcgacca ggtactcgct ctggcccagg
     4681 cgggcgccgg tgccgagcag ccgcagacgg ccgccgacga tgacggtcgg gtcgagcccg
     4741 gcgtcggtga gcacggtccc caccagcgag gtggtggtgg tcttgccgtg ggtaccggcg
     4801 attccgatac cgtatcgcag ccgcatcagc tcgcccagca tctctgcccg ccgcaccacc
     4861 gggatgccga gccgccgcgc ctcgcgcacc tcggcgttgt cctccgccac cgccgacgag
     4921 atcaccacca ggtcgacgcc gtcgaggtgg tcgggcgagt gcccctggtg gacggtgatg
     4981 cccagctcga tgagccgctg cgtcgcctcg gagagtgact ggtcgcagcc gctgaccacc
     5041 aggttcgaca ggctgtaggc gcgcaggatc tcggcgatcc cgctcatgcc ggcgccgccg
     5101 acgccgacga agtggatgtg cgacaggtcg gtgaactgat tgaacatcag ccctcccctc
     5161 ccagctcctc gagccggctc gcgaccgccg ccgccgcccc ggggcgggcc agcgatcgcg
     5221 ccgcccggcc catgcgcgcc aggcgctcgc cgtcggcgag gagctccgcc agcaggtccg
     5281 ccagtccctc ggaggtcacc tggtccggcg tccgcatctc ggcggccccc tcctcggcca
     5341 ggagacgtgc gttgtccacc tgatgcgcct tggcgatcac aagcggcagc agcaccgatg
     5401 gccggccggc ggcgcagatc tcggcgacgg tgatggcgcc ggcgcgggag accagcaggt
     5461 ggctcgccgc catggcgccg gctacgtcgt ccaggaacgg gacgacctca acgtgccccg
     5521 gcccgacgcc cgcgtcagcg taggcctgcc gcgtctcctc caggttcttc tcacccgcct
     5581 ggtggagcac cgacagctcc ggcaggcgct ggacgagccg cgccaccgct gccggcaccg
     5641 tctgattgat ctgccgcgcc ccctggctgc cacccagcac cagcagccgt ggtgctgtaa
     5701 gagaggacga cagcgggggc acggcgaaga aggcggcgcg caccggcacg ccggtcaccc
     5761 agcaggggca cctgaggtcg ccaatcgtgt cgcggtagcc cacggccgcg ccggtggccc
     5821 agcgggagag ccagcggttg gcgacgccgg ctcgggcatt gggctcgagc agcaggatcg
     5881 gccgcccggc gaagcgtgca ccaaggaccg ccggcgccga gacatagccg ccggtgccca
     5941 gcaccgcgtc gacgccgagg cgccggatca gtgccgaggc cgcctgtgcc gagccggcca
     6001 gtgtggtcag cgcctgggct ttgccaaaga ggccgcgccc gaggagcgga cgcgccggca
     6061 gcggatggaa cggaataccc cgcgccggc
//
LOCUS       DASPJI010000696         3063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_677030, whole genome shotgun sequence.
ACCESSION   DASPJI010000696 DASPJI010000000
VERSION     DASPJI010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677030"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VEL74_19675"
     CDS             <1..1126
                     /locus_tag="VEL74_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HYG64810.1"
                     /translation="LESIVCKQRVDEVWFSYSDVSHEELMHKGSRAVAWGANFGICSA
                     TRTMLASSRPVIAVTAVRTGVGKSQTTRYLSRVLRDLGQEVVAVRHPMPYGDLAKQVW
                     QRFATYEDLDLQQTTIEEREEYEPLIDNGFVVYAGIDYQEILRRAEEEAEVILWDGGN
                     NDTPFFKPDLHICLLDPHRPGHETSYYPGETNFVMADLLIINKVDTADPGDVERLLES
                     CRRHNPKARVIQCRSAIQVDDPGAIRGKRALVIEDGPTLTHGGLSRGAGWYAARQAGA
                     AEIVDAAPHAVGSIRSTYEKYPNARGILPAMGYGDNQVRELEATINATPADVVVEGTP
                     IDLGRVLSVNKPMVSVRYELEELEPGVLAEAVRGVLGGGR"
     gene            complement(1136..>3063)
                     /locus_tag="VEL74_19680"
     CDS             complement(1136..>3063)
                     /locus_tag="VEL74_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HYG64811.1"
                     /translation="RDVSERRRAEEAQARLAAIVESSQDAIVSKSLEGIIRTWNAGAE
                     RLFGYSAEEAVGRSITLLIPEDRLDEERRILAQIARGERIEHYETIRVSKDGRRLDIS
                     LTISPLRDSEGRIIGASKVARDVTGRRQAEEAQARLAAIVESSHDAIISKTLDGVIRT
                     WNTGAERLFGYTAEEAVGRPITLLIPPDRLDEERMILARLARGEQIEHYETVRVAKNG
                     RPIAISLTISPIRDSEGRIIGASKVARDVTDRKQAEEALRAAMEALREADRRKDEFLA
                     LLAHELRNPLAPIRNGLQVMRLAAGDAQAIARSRDIMDRQLSHLVRLVDDLLDVSRVS
                     RGKMELRRSRVLLADVVSSALEIVRPAAKAAGLELTVSLPPQPVYLDADLTRLAQVFG
                     NLLHNSVKYTEPGGHIWLTAAQEGGQVSVVVRDTGIGIPAFALPTIFEMFSQVDRSIE
                     RSTGGLGIGLSLVKGLVEMHGGTVEAASPGEGQGSTFTVRLPVLRDRAEAPLGAQDEA
                     KTEATQEKRRILVVDDNVDAANSLAMMLEVLGDEVRTAHDGAEAVELAEQFRPQVVLM
                     DIGMPKLNGYEATRRIREEPWGRTMVIIALTGWSQEVDRVKSKEAGCDGHLVKPVHLS
                     DLENLLADLQGPAPRPG"
BASE COUNT          443 a         1106 c         1036 g          478 t
ORIGIN      
        1 gctggagtcg atcgtctgca agcagcgggt ggacgaggtc tggttctcgt actcggacgt
       61 ctcgcacgag gagctgatgc acaaggggag ccgcgcggtg gcctgggggg cgaacttcgg
      121 gatctgctcg gccacccgca ccatgctcgc ctcctccagg ccggtgatcg cggtaaccgc
      181 ggtgcgcacc ggcgtgggca agtcgcagac cacgcggtat ctctcgcgcg tcctgcggga
      241 cctgggccag gaggtggtgg cggtgcgcca tcccatgccc tacggcgacc tcgccaagca
      301 ggtctggcag cgcttcgcca cctatgagga cctggacctc cagcagacga ccatcgagga
      361 gcgggaggag tacgagccgc tgatcgacaa cggcttcgtg gtctacgccg gcatcgacta
      421 ccaggagatc ctccgccggg ccgaggagga ggccgaggtg atcctctggg acggcggcaa
      481 caacgacacg ccgttcttca agcccgacct ccacatctgc ctgctcgacc cccaccggcc
      541 ggggcacgag acgagctact acccgggcga gaccaacttc gtgatggcgg acctgctgat
      601 catcaacaag gtggacacgg cggacccggg ggacgtggag cggctgctgg agagctgccg
      661 gcgccacaac cccaaggcgc gggtgatcca gtgccgctcg gcgatccagg tggacgaccc
      721 cggcgcgatc cggggcaagc gggcgctggt gatcgaggac ggccccaccc tcacccatgg
      781 gggcctgagc cgcggcgccg gctggtacgc cgcccggcag gccggggcgg cggagatcgt
      841 ggacgccgcg ccgcacgccg tcggctccat ccgctcgacc tacgagaagt acccgaacgc
      901 ccgcggcatc ctccccgcca tgggctacgg cgacaaccag gtccgggagc tcgaagccac
      961 catcaacgcc acgccggccg acgtggtcgt cgaggggact cccatcgacc tcggacgcgt
     1021 cctctcggtc aacaagccga tggtctcggt gcgctacgag ctcgaagagc tggagcccgg
     1081 cgtcctcgcc gaggcggtgc ggggggtgct gggggggggg cgatagcggc cggcgtcacc
     1141 cggggcgggg tgccggcccc tgcagatcgg cgaggaggtt ctccagatcg gacaggtgga
     1201 ccggcttcac caggtggccg tcgcagccgg cctccttcga cttgacgcgg tccacctcct
     1261 ggctccagcc ggtcagtgcg atgatgacca tcgtgcgtcc ccagggctcc tcgcggatgc
     1321 ggcgggtcgc ctcgtagccg ttgagcttgg gcatcccgat gtccatcagg accacctgtg
     1381 gacggaactg ttcggccaac tccacggcct cggctccgtc gtgcgcggtg cggacctcgt
     1441 cccccagcac ttcgagcatc atggccaacg agttggccgc atcgacgttg tcgtccacca
     1501 ccaggatccg tctcttctcc tgggtggcct ccgtctttgc ctcgtcctgt gccccgagcg
     1561 gcgcctccgc ccggtccctc agcaccggaa gcctgacggt gaaggtgctc ccctgcccct
     1621 ctcccgggct cgcggcctcg accgtgccgc cgtgcatctc gaccaatccc ttgaccaggg
     1681 acagcccgat gccgagcccg ccggtggacc gttcgatcga ccggtccacc tggctgaaca
     1741 tctcgaagat cgtcggcagg gcgaaggcgg ggatgccgat cccggtgtct cgcaccacta
     1801 ccgacacctg acccccttcc tgggccgcgg tgagccagat gtgcccgccc ggttcggtgt
     1861 acttgacgct gttgtggagc aggttgccga acacctgcgc caaccgggtc aggtcggcgt
     1921 ccagatagac cggctgcggc ggcagcgaga ccgtgagctc gagcccggcc gccttggccg
     1981 ccgggcggac gatctccagg gcgctgctga ccacgtcggc caacagcacc cgggagcggc
     2041 gcagctccat cttgccgcgg ctgacccggg agacgtcgag caggtcatcc accaaccgca
     2101 ccaggtgcga gagctgccgg tccatgatgt cccgcgacct ggcgatcgcc tgcgcgtcgc
     2161 cagccgccag ccgcatcact tgcaggccgt tccgtatcgg agccagggga ttcctgagct
     2221 cgtgagccag cagcgcgagg aactcgtcct tgcgccggtc ggcctccctg agcgcctcca
     2281 ttgccgcccg cagggcctcc tcggcctgct tccggtcggt cacgtcgcgg gcgaccttcg
     2341 acgcgccgat gatccgcccc tccgagtcgc ggatcggaga gatggtcagc gagatggcga
     2401 tcggccggcc gttcttggcg acccgcaccg tctcatagtg ctcgatctgc tccccgcgcg
     2461 cgagccgggc caggatcatc cgctcctcgt ccagccggtc cggcgggatg agcagcgtga
     2521 tcggccggcc cacggcctcc tcggccgtat atccgaagag ccgctcggcc ccggtgttcc
     2581 acgtccggat gacgccatcc agcgtcttgc tgatgatcgc gtcgtgggac gactcgacga
     2641 tggcggcgag ccgcgcctgg gcctcctcgg cctggcggcg cccggtcacg tcgcgcgcga
     2701 ccttcgacgc gccgatgatc cgcccctcgg aatcgcggag tggagagatg gtcagcgaga
     2761 tgtcgagccg ccggccgtcc ttgctgaccc ggatcgtctc gtagtgctcg atccgctccc
     2821 cgcgcgcgat ctgggccagg atcctccgct cctcgtccag ccggtcctcg gggatgagca
     2881 gcgtgatcga ccggccgacc gcctcttcgg cgctgtaccc gaagagcctc tcggctccgg
     2941 cgttccacgt ccggatgatg ccttcgagac tcttgctgac gatcgcgtcc tgggacgact
     3001 cgacgatggc ggcgagccgc gcctgggcct cctcggcccg ccggcgctcg ctcacgtcgc
     3061 gga
//
LOCUS       DASPJI010000697         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_391780, whole genome shotgun sequence.
ACCESSION   DASPJI010000697 DASPJI010000000
VERSION     DASPJI010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391780"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VEL74_19685"
     CDS             <1..1225
                     /locus_tag="VEL74_19685"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYG64812.1"
                     /translation="SGMRSWLRVPVWLSGEVRGSLGFFHRETARYDRGDVEVASRLAD
                     RVALMLSHQRLAEEAQVAAEVRERAERLQATVETLTRELEARGRGRVVGTSASWREVL
                     LQVGRVASSETTVLITGESGTGKEVVAHLLHEGSRRSGKPFVAINCAALPEQLLESEL
                     FGHERGAFTGAITAKVGRLEQAAGGTLFLDEIAEMSPVVQAKFLRVLQEREFQRLGGT
                     RTLRADVRVIAATNRDLGQAIGRQQFRDDLYYRLNVFGIHIPALRDRPEDVLLLAQTF
                     LRDLGRTMGRPAAGISRDAREWLLAYHWPGNVRELRNAIERAILLCDGGLITREHLPA
                     ALGRPEPARADLVILGDAAAPLPTGGVDLEDVERSFIERALQTARGNKSKAARLLGLT
                     RAQLYSRLEKYSLTA"
     gene            1463..1714
                     /locus_tag="VEL74_19690"
     CDS             1463..1714
                     /locus_tag="VEL74_19690"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HYG64813.1"
                     /translation="MSQSNVERVIGRLVTDEEFRRRFSEAPSAVLREWREGGLELTEC
                     EFRVLSAFDCQVAARCASVIDPRLQKADLTGGEKRCGSE"
     gene            1699..>2221
                     /locus_tag="VEL74_19695"
     CDS             1699..>2221
                     /locus_tag="VEL74_19695"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYG64814.1"
                     /translation="MRFGIAVRTIGMALVASWMVPAAGIAQMVERPLTLTEAIALALE
                     NNEGIRIERALLESAEAGILGAQGAYDPLVEVEGGWRRSSLPVNSSFSGAPSGQLAPT
                     TESFYTDATVSQLLPTGARAAVRGSADRGSTDGVFDLLSPSYGTGLGFEVRQPLLRDR
                     RVDAARIAIRVAAA"
BASE COUNT          310 a          793 c          788 g          330 t
ORIGIN      
        1 ctcgggcatg cggtcctggc tgcgggtccc cgtctggctg tcgggcgagg tgcggggcag
       61 cctcggcttc ttccaccgcg agaccgcccg ctatgaccgc ggggacgtcg aggtcgccag
      121 ccggctcgcc gaccgcgtcg ccctgatgct ctcccaccag cggctcgccg aggaggccca
      181 ggtggccgcc gaggtacggg agcgcgccga gcggctgcag gcgacggtcg agactctcac
      241 ccgcgagctg gaggcccggg gacgcggccg ggtcgtcggc acctcggcct cctggcggga
      301 ggtgctcctc caggtcgggc gcgtcgcctc ctcggagacg acagtgctca tcaccggcga
      361 gtccgggacc ggcaaggagg tggtcgccca cctcctccac gagggctcgc gccgttccgg
      421 caagcccttc gtggcgatca actgcgccgc gctgccggaa cagctcctcg agtcggagct
      481 cttcggccac gagcgggggg ctttcaccgg cgcgatcacg gccaaggtcg gccgtctcga
      541 gcaggccgcc ggcggcaccc tcttcctcga cgagatcgcc gagatgagcc cggtggtcca
      601 ggccaagttc ctgcgcgtcc tccaggagcg cgagttccag cgcctggggg ggacgcgcac
      661 gctgcgcgcc gacgtgcggg tcatcgcggc caccaaccgc gacctgggcc aggcgatcgg
      721 ccggcagcag ttccgggacg acctctacta tcggctgaac gtgttcggga tccacatccc
      781 ggccctgcgc gaccgccccg aggacgtcct cctgctcgcc caaacttttc tgcgggacct
      841 ggggcggacc atgggccggc ccgccgccgg catctcgcgc gacgcccgtg agtggctgct
      901 cgcgtatcac tggccgggca acgtccgcga gctgcgcaac gccattgagc gcgccatcct
      961 gctctgcgac ggcggcctga tcacccgcga gcacctcccc gccgccctcg gccggcccga
     1021 gccggcgcgc gccgacctgg tcatcctcgg ggacgccgcc gcgcccctcc ccaccggcgg
     1081 cgtggacctg gaggacgtcg agcggagctt catcgaacgc gccctgcaga ccgcccgcgg
     1141 caacaagtcc aaggcggcaa ggctcctcgg cctcacccgc gcccagctat actcgcggct
     1201 cgagaagtac agcctgaccg cctgaccccc ggggtgcggc cccgacgaca gactgtcgat
     1261 ttttcgacaa cttcaccgta gcccccaagt cttccggagc ccctcggcct caggccgaac
     1321 tccagtattg acaagaagtt gcgcaccgcg tgccggcatt gaccggcgat tggcagcgct
     1381 ttggctcctg agggggagaa ggctggcggc tgccccctgt cgaaatcccg gggggccgtt
     1441 cgaccactag ctggaggagg agatgagtca gtccaacgtc gagcgggtga ttgggcggtt
     1501 ggtgaccgac gaggagttcc ggcgccggtt cagcgaggcc ccgtccgcgg tgctgcggga
     1561 gtggagggag ggcggcctgg agctgaccga atgcgagttt cgcgtgctct cggctttcga
     1621 ctgccaggtc gcggcgcgtt gtgccagcgt catcgacccg cggctccaga aggccgatct
     1681 cactggagga gaaaaacgat gcggttcgga atagcggtgc ggacgatcgg gatggccctg
     1741 gtggcgtcct ggatggtacc ggccgcaggc attgcacaga tggtggagcg tcccctgacg
     1801 ctcacggagg ccatagcgct cgccctcgag aacaacgagg ggattcggat cgagcgtgcg
     1861 ttattggagt cggcggaggc cggcatcctg ggtgcccagg gtgcctatga cccgttggtc
     1921 gaggtcgagg gcggctggcg gcggagcagc ctgccggtca actcgtcgtt ctccggcgcc
     1981 ccgtcaggac agctggctcc gaccacggag tccttctaca cggacgccac ggtcagtcag
     2041 ctcctgccga cgggcgccag ggccgcggtc cgcggctcgg ccgaccgcgg tagcaccgac
     2101 ggcgtcttcg acctgctctc gccgtcctac gggaccggcc tgggcttcga ggtccgccag
     2161 ccgctgctgc gggaccggcg ggtcgacgcg gcccggatcg ccatccgggt cgccgccgcc
     2221 g
//
LOCUS       DASPJI010000698         2613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_45005, whole genome shotgun sequence.
ACCESSION   DASPJI010000698 DASPJI010000000
VERSION     DASPJI010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45005"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VEL74_19700"
     CDS             complement(<1..779)
                     /locus_tag="VEL74_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64815.1"
                     /translation="MSPRSRVPSSIPAVLLVLTAALCAPPAFAQDPASTPPPAPPPAA
                     AQAAAQPPLPEEAPQEQVQAAPVPQTQSGVPPQPQVQAAEPATGDTPATTPPPPKDGF
                     LPRLDIFFPEGDLDLKVSRLINSTFFEGQVKYNFVKGDINAFLRYRYYGYSRITQLTV
                     FDSVEFEDLEDFSSDFDRVRGALLLLQWPHSYSYRTYAVLELDRISSNKRDLQFEETE
                     EALVRQGTTNTFVRLGYQVGTPGDERSNAIVGESRARNRQL"
     gene            complement(855..1676)
                     /locus_tag="VEL74_19705"
     CDS             complement(855..1676)
                     /locus_tag="VEL74_19705"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HYG64816.1"
                     /translation="MAFQGSLKELPLPDIIQLVSVSGKTGVFSLKRDGDSAGEIFLRG
                     GQIVHAQVGDLKGEEAVYELAIWPEGEFVFTPGRETELTTIQKSNTNLLMEAARRIDE
                     WQILSKRIPSTRLVPVFTNQAATTSVSLTPPEWALICKIDERRTIEEIAIGLDMSPFE
                     VCKLLYGLITSGLLALNEDLSRLRTDRLRNMPPEELAQLVDEVHQQGRTLLSGHGRQS
                     ELDVPYRMSKAELDSGRGVEAILDLVRAEEKVVSAALGPNQSKTFLDRVSQILNR"
     gene            complement(1805..2332)
                     /locus_tag="VEL74_19710"
     CDS             complement(1805..2332)
                     /locus_tag="VEL74_19710"
                     /inference="COORDINATES: protein motif:HMM:NF014166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HYG64817.1"
                     /translation="MLILGLDPGSLHTGYGLVEKRGSSVRAVDVGRLSCPRGLAVPQR
                     LAHLASCLGDLLTRRPPDLAVLESPFAGLNARSLIVLAEARGALLAVLAGRGIEIREY
                     SPSEVKSAVTGNGRADKEQVARMVRLLLGAAAGDGAGTVGGQRWASDATDALAVALCC
                     AQRLRMDRLRDPVRA"
     gene            complement(2346..>2613)
                     /locus_tag="VEL74_19715"
     CDS             complement(2346..>2613)
                     /locus_tag="VEL74_19715"
                     /inference="COORDINATES: protein motif:HMM:NF013841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HYG64818.1"
                     /translation="ADDFAVEADHYELYTSMEDFARVQEELERRNVPLAAKELAMVPQ
                     TWVDVPSDKVNLLMRLLEALEDHDDVQKVWANLNVDEKILAGQA"
BASE COUNT          375 a          844 c          990 g          404 t
ORIGIN      
        1 aagagctgcc ggttgcgcgc ccggctctcc ccgacgatgg cgttggagcg ctcgtccccg
       61 ggggtgccga cctgatagcc caggcgcacg aaggtgttgg tcgtcccctg ccggaccagc
      121 gcctcctcgg tctcctcgaa ctgcaggtcc cgcttgttgg agctgatgcg gtcgagctcc
      181 agcacggcat aggtgcggta gctgtagctg tgcggccact gcagcaggag cagggcgccg
      241 cgcacccggt cgaagtcgct ggagaagtcc tccaggtcct cgaactccac cgaatcgaag
      301 acggtgagct gggtgatgcg gctgtagccg tagtagcggt agcgcaggaa ggcgttgatg
      361 tcgcccttga cgaagttgta cttgacctgc ccctcgaaga aggtgctgtt gatcagccgg
      421 ctgaccttga ggtccaggtc cccctcgggg aagaagatgt cgaggcgcgg caggaagccg
      481 tccttgggcg gcggcggagt cgtcgccggc gtgtcgccgg tggccggctc ggccgcctgg
      541 acctgcggct gcggcggcac gccggactgg gtctgcggca ccggagccgc ctggacctgc
      601 tcctgcggcg cctcttccgg gagagggggc tgcgccgcgg cctgcgctgc cgccgggggc
      661 ggcgcgggcg gcggcgtgct cgcggggtcc tgggcgaagg ccggcggcgc gcagagcgcg
      721 gcggtcagga ccaggaggac ggccggaatg ctggacggaa ctcggctccg tggtgacatg
      781 gctgggctct cggagtttct ctccctgcgg atctcccgtg caaacggcgt gcacagggag
      841 acggccgggc tcggtcagcg gttcaggatc tgggagacgc ggtcgaggaa ggtcttgctc
      901 tggttcgggc cgagggcggc ggagaccacc ttctcctcgg cgcggacgag gtcgaggatg
      961 gcctccacgc cacgtccgga gtccagctcg gccttgctca tgcggtaggg gacgtcgagc
     1021 tcggactggc ggccgtggcc ggagagcagc gtccgtccct gctggtgcac ctcgtcgacg
     1081 agctgcgcca gctcctccgg cggcatgttg cgaaggcggt cggtgcgcag gcggctcagg
     1141 tcctcgttca gggcgaggag cccggaggtg atcaggccgt agaggagctt gcagacctcg
     1201 aacgggctca tgtcgaggcc gatggcgatc tcctcgatgg tccggcgctc gtcgatcttg
     1261 cagatcaggg cccactccgg cggcgtcagc gacaccgacg tcgtcgccgc ctggttggtg
     1321 aagacgggca ccaggcgggt ggaggggatg cgcttcgaca ggatctgcca ctcgtcgatc
     1381 cgccgcgccg cctccatcag gaggttggtg ttcgacttct ggatggtggt gagctcggtc
     1441 tcgcggccgg gggtgaagac gaactccccc tcgggccaga tggcgagctc gtagaccgcc
     1501 tcctccccct tcaagtcccc cacctgggcg tggacgatct ggccgccgcg caggaagatc
     1561 tcgcccgcgg agtcgccgtc gcgcttcagg gagaacacac cggtcttgcc cgagaccgag
     1621 acgagctgga tgatgtcggg aagcggaagc tctttgaggg acccctggaa ggccatggcg
     1681 cgcgctaggg ctgggagctc acagtcgaat tggagaacaa aggcacggga tatccgtcaa
     1741 ggcccggatc gtagcacagc cccggacggg gtcaaagcca cgggccgtca tcggtttacg
     1801 cgcgttacgc gcggacgggg tcgcgcagcc ggtccatgcg caggcgctgg gcgcagcaga
     1861 gggccacggc cagggcgtcg gtggcgtcgc tcgcccagcg ctgacctccg acggtcccgg
     1921 cgccgtctcc cgccgccgcc ccgagcagca gccgcaccat gcgggcgacc tgctccttgt
     1981 cggcgcggcc gttgccggtg accgcggact tgacctcgga ggggctgtac tcgcggatct
     2041 cgatcccccg gccggcgagc acggcgagga gcgccccccg cgcctcggcg agcacgatga
     2101 gggagcgggc gttcaggccg gcgaaggggg attcgaggac cgccaggtcg ggggggcgcc
     2161 gggtgagcag atcccccagg caggaggcga ggtgggcgag ccgctggggc acggcgaggc
     2221 cgcgcgggca ggagaggcgg ccgacgtcca ccgcccgcac cgaggagcct ctcttctcca
     2281 ccaggccgta gccggtgtgg aggctgccgg gatcgaggcc caggatcagc atgaggcgct
     2341 cagcatcagg cctgcccggc caggatcttc tcgtcgacgt tcaggttggc ccacaccttc
     2401 tggacgtcgt cgtggtcctc cagggcctcc agcaggcgca tgagcaggtt gaccttgtcg
     2461 ctggggacgt cgacccaggt ctgggggacc atcgccagct ccttggcggc gagcggcacg
     2521 ttgcggcgct ccagctcctc ctggacgcgg gcgaagtcct ccatcgaggt gtagagctcg
     2581 tagtgatcgg cctcgacggc gaagtcgtcg gcg
//
LOCUS       DASPJI010000699         4475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_444024, whole genome shotgun sequence.
ACCESSION   DASPJI010000699 DASPJI010000000
VERSION     DASPJI010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444024"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VEL74_19720"
     CDS             <1..458
                     /locus_tag="VEL74_19720"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64819.1"
                     /translation="LGPRLYRSGDGGQSWQRRDAGLPGTVTRLALDPERSDVLFAAAG
                     DGLFASSDGGATWQRLPSPATVSASPESIRWEIVASPEAPLTLYAHLDGLESEVVYRS
                     GDGGATWQAVGGSVAPGSFGIRALTVDPHDPRRLLAGTAKRGIFTFTEP"
     gene            1212..3800
                     /locus_tag="VEL74_19725"
     CDS             1212..3800
                     /locus_tag="VEL74_19725"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HYG64820.1"
                     /translation="MWGRRPTNRRPGLLPALLLILGVIPPAAAQDQVREWRRWETVLT
                     SARDYTANDGNPYRDLVVEVTFQRDSPFLQVSGVGFWDGGRTFKVRAALPAGTWRWTT
                     SCVGATGGQSCGGPDGSDRDQGLWRTGSVRVLPGAGGADVPEIYRRGFLRAGGRTLLH
                     GDGTPFYWLGDTAWQAPVSATREQWATYLKDRAAKGFTVVQIAPATQYEGEITSFEQI
                     GDCPSPAIPNRCSRWVPEFWRHVDALVEEANRAGIVVAVAGLINPVGRGGTRKADLYP
                     APEDAAVFARNLAARLAGNFVIFSPGFDDRIDIQISPEVTTRDSMEAVGRELEEVAPR
                     HLVANHLAGASPVSDYAVFQDQSWLSFQLFQSGHALNQSACGRGETRQQCAAQRAREL
                     PLGLWRLSPVRPAANGEGGYEDPLNPEAAPPDNRFGVRQIAYSSLLSGAFGFTLGVKG
                     IYFWDKPMEALDSSGSLDMQRLGGFFRAWPWAELVPAPERILGNDAQPQQRRMALAGT
                     PDESLAVAYLPNNDSIAVATGTAGLGCGPDWSRLWLDPRTGESQPVDGCTEAPGRITL
                     TRPACQDPNPDVTGGCDWLLELRDTGARLSANSMTGEKAAGANAMQVWAAPAATEPGK
                     RSSSIRARVFGPDGKVVQDGIVVSPAGEALQTLPVVARDARGGFFVAWQTGSGIFARR
                     FDSRGRPLDRAFRVDRSRRAGGAPVVTADARGRVVVVWVSRGDVFAQRFGANGGRMGA
                     PFRVNVHNAGEQSAPRVMADAAGNFVVAWRSDGQDGDGRGVFARLFDRRGRPRTAEIQ
                     VNRAAAGDQELTGLTVTQQGRFAVRWATRPFAGGTGGRRVPLEQWFDRDGRRLGEART
                     PVQSNP"
     gene            3797..>4475
                     /locus_tag="VEL74_19730"
     CDS             3797..>4475
                     /locus_tag="VEL74_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64821.1"
                     /translation="MKYPAHSRLLGFTLTALLGVSLAAAAPPEPFGETTDVVAVEVPV
                     QVVRDGQPVRGLTAADFEVHEGRKKQPIVGFDVIDLAATPGATSATSATSAAQAQPAA
                     VPAAARRHFLLLFDLSFSEPKSIGKAREAARDLVKGKLQPADLVAVATYSSRGAQLVL
                     GFSPDRRQVDRAIDAVGLPELIDRNPDPLKLVAGEVEDMLASGYKAVDNEKMLEEIEA
                     AVRESERA"
BASE COUNT          628 a         1561 c         1611 g          675 t
ORIGIN      
        1 cgctggggcc gcgcctctat cgcagcggcg acggcgggca gagctggcag cggcgcgacg
       61 ccgggttgcc cggcacggtg acgcgcctcg ccctcgatcc cgagcgctcc gacgtgctgt
      121 tcgccgccgc cggcgacggg ctgttcgcca gcagcgacgg cggcgccacc tggcagcggc
      181 tgccgtcccc tgcgacggtg tcggcgagcc cggagagcat ccgttgggag atcgtcgcct
      241 cgccggaggc cccgctgacg ctctatgccc atctcgacgg gctcgagagc gaggtggtgt
      301 accgcagcgg cgacggcgga gccacctggc aggcggtcgg cggcagcgtc gcccccggct
      361 ccttcggcat ccgcgccctg accgtcgatc cccacgaccc gcggcggctg ctcgccggca
      421 cggccaagcg cggcatcttc accttcaccg agccgtaggg ctcgcggcac gggcggtacc
      481 aggcggagca cttcaagaag taagagggtg ctccgtcctg gagccttctc aatgaaagga
      541 gaatcgcttg agaagctctg ccaccgctcg cctgcttctg ctcgtagtcg ccatccccct
      601 gtgcctcctg gcactgccgg actccgccga agcgttccct tcctgctctt cgatctggat
      661 ctcttgcagt tactacgaat ccttgtattg cccgaacggc gtcatgagcc tcacgcccgt
      721 agggttctgc caggatgagt accagtacct gtacgattat tacatcgcac ggtgcaacgg
      781 cgaattcccc atggagtgca atccccacat gctttaggtc gcaccacggc gccaccggcg
      841 ggaggcccgc cggcggcgcc tctctcgctt ccctccggac tggatggctc agccgctgca
      901 gccgagggaa ttggggctgt tcggatcgtg gacatccgcc cagcactccc cgcagacgat
      961 ttcgcagcag acgggcatcc cgatgcaatc gcaggaggtg caatcgcagg agtacatgct
     1021 gcagagcccc ttcgacgagg catccggttg gccgacggcc aggaacagga aggtacagag
     1081 gattccgata cggagggctt ggtgcactcg agtcatttct ttctccttca atgaggtcgg
     1141 tgcggaaagc tctgggcgcg tgacagacag gtggatacgg ctcgcctcga ccggatagag
     1201 tcggtcccgt gatgtgggga cgaagaccaa cgaaccgccg cccggggctc ctgccggccc
     1261 tgctgctgat cctcggggtg atcccgccgg ccgccgctca ggaccaggtc cgggagtggc
     1321 ggcgctggga gacggtcctc accagtgcgc gcgattacac ggcgaacgat ggcaacccct
     1381 accgtgacct cgtggtcgag gtgaccttcc agcgcgattc gccgttcctc caggtttcgg
     1441 gggtcgggtt ctgggatggc ggcaggacgt tcaaggtccg ggcggccctg cccgcgggga
     1501 cttggcgctg gaccacgagc tgcgtcggcg cgacgggtgg acagagttgt ggcggtcccg
     1561 acggctcgga ccgcgatcag gggctttggc ggaccggcag cgtccgggtg ctccccggcg
     1621 ccggaggggc cgacgtgccc gagatctacc ggcggggctt cctccgtgcg gggggccgga
     1681 ccctcctcca tggtgacggc acgccgttct attggctggg cgacaccgcc tggcaggcgc
     1741 cggtcagcgc cactagggag cagtgggcca cctatctgaa ggatcgcgcg gccaaggggt
     1801 tcacggtcgt gcagatcgcc ccggcgaccc agtacgaggg ggagatcacc tcgttcgagc
     1861 agatcggcga ctgcccgagc ccggcgatcc ccaaccgctg ctcgcgctgg gtccccgaat
     1921 tctggcgcca cgtcgacgca ctcgtcgagg aggcgaaccg ggccgggatc gtggtcgccg
     1981 tcgccgggct gatcaatccg gtgggccggg gcgggacgag gaaggcggac ctctaccccg
     2041 cgcccgagga cgcggcggtc ttcgcccgca acctggcggc gcggctggcc ggcaacttcg
     2101 tgatcttctc tcccggattc gatgaccgga tcgacatcca gatctccccc gaggtgacca
     2161 cccgcgactc gatggaggcg gtggggcgcg agttggagga ggtggcgccg cggcacctgg
     2221 tagccaatca cctggccggg gcgtcgccgg tctccgacta tgccgtgttc caggaccaga
     2281 gctggctctc cttccagctc ttccagagcg ggcacgccct gaaccagtca gcctgcgggc
     2341 gcggcgagac ccgccagcag tgcgcggcgc agcgggcccg cgagctcccc ctggggctct
     2401 ggcgcctctc cccggtccgg cccgccgcca acggcgaagg gggatacgag gacccgctga
     2461 atcccgaggc ggcgcccccc gacaaccgct tcggggtgcg ccagatcgcc tactccagcc
     2521 tcctctcggg agcgttcggc ttcaccctcg gggtcaaggg gatctacttc tgggacaagc
     2581 cgatggaggc cctcgactcc tccggctcgc tcgacatgca gcgtctcggc gggttcttcc
     2641 gggcctggcc ctgggcggag ctggtccccg cgcccgagcg gatcctcggc aacgacgccc
     2701 agccgcagca gcggaggatg gcgctcgccg gcacgcccga cgagagcctg gcggttgcct
     2761 atctccccaa taatgattcc atcgccgtcg ccaccggcac cgccggcctc ggctgcggtc
     2821 ccgactggag ccggctgtgg ctcgatcccc ggaccggcga gtcccagccg gtcgacggct
     2881 gcaccgaggc gccgggccgg atcaccctga cgcggccggc ctgccaggac ccgaatcccg
     2941 acgtcacggg cggctgtgac tggctgctgg agctgcgcga caccggcgcg cgcctctcgg
     3001 ccaactccat gacgggggag aaggcggccg gagccaacgc gatgcaggtc tgggccgcgc
     3061 cggccgctac ggagccgggg aagcggtcat cgtcgatccg ggctcgggtc ttcggtccgg
     3121 acggcaaggt cgtccaggac ggcatcgtgg tgagcccggc cggcgaggcc ctccagacgc
     3181 tccccgtggt agcccgggac gcccggggcg gtttcttcgt cgcttggcag acgggctccg
     3241 ggatcttcgc gcggcggttt gattcccggg gccgtccctt ggatcgagcc ttccgggtgg
     3301 accgctcccg gcgggccggc ggcgcaccgg tggtgacggc ggatgcccga gggcgggtgg
     3361 tggtggtctg ggtgagccgg ggggacgtct tcgcccagcg cttcggcgcc aatggcggcc
     3421 gaatgggcgc cccgttccgg gtcaacgtcc ataacgcggg ggagcagagc gcgccgcggg
     3481 tgatggcgga cgcggccggc aacttcgtgg tcgcctggcg cagcgacggc caggatggcg
     3541 acggccgggg cgtctttgcc cgcctcttcg accgccgcgg ccgcccccgg acggcggaga
     3601 tccaggtgaa ccgggccgcc gccggcgacc aggagctgac cgggttgacc gtcacccagc
     3661 aggggcggtt cgcggtccgc tgggcgaccc ggccgttcgc cggcgggacc ggcgggcggc
     3721 gggtgccgct ggagcagtgg ttcgaccgcg acggccgccg gctcggagag gcgcggacgc
     3781 ccgtacaatc gaatccatga aatacccggc gcactcgcgg ctcctcggct tcaccctcac
     3841 ggctctcctc ggtgtctcct tggcggctgc cgcgcctccc gagcccttcg gcgagacgac
     3901 ggacgtcgtc gccgtcgagg tgcccgtcca ggtggtgcgg gacggccagc cggtgcgcgg
     3961 gttgaccgcc gcggacttcg aggtccacga gggccgcaag aagcagccga tcgtcggctt
     4021 cgacgtcatc gatctggcgg cgaccccggg ggcaacatcg gcgacatcgg cgacatcggc
     4081 ggcgcaggcc caaccggccg cggtcccggc cgcggcccgg cgccatttcc tgctgctgtt
     4141 cgatctctcg ttctccgagc cgaagtcgat cggcaaggcg cgggaggcgg cgcgcgatct
     4201 ggtcaagggg aagctccagc cggccgacct ggtggcggtg gcgacgtaca gctcccgcgg
     4261 cgcccaactc gtcctcgggt tcagccccga ccgccggcag gtcgaccgcg ccatcgacgc
     4321 cgtcgggctg ccggagctga tcgaccgcaa tcccgacccg ttgaagctgg tggcggggga
     4381 ggtcgaggat atgttggcct cgggctacaa ggcggtcgac aacgagaaga tgctcgagga
     4441 aatcgaggcc gcggtccggg agtcggagcg cgccg
//
LOCUS       DASPJI010000700         5827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_392070, whole genome shotgun sequence.
ACCESSION   DASPJI010000700 DASPJI010000000
VERSION     DASPJI010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392070"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5827)
                     /locus_tag="VEL74_19735"
     CDS             complement(<1..>5827)
                     /locus_tag="VEL74_19735"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64822.1"
                     /translation="SMARLRLPFEARAGAGTEVKAEVQLSTVRRQLVPAALEEAAGRC
                     GAPGQAFLLAAWLAFVGRITGEREVVVGAVRPGRDHELLQGVLGPMALCHPVHSGLEE
                     GLRFLALVQRVDVALQEGAEWQESFRIEEALPPDEAGGPPYFPFGFAWEERQIRRSAG
                     PLSFLQVERFLSAERFDLALCCAAGPGSLLLELQIDSGRWTTRYAEILAAQFETLLAA
                     AIRDPEARIDELEMLPEDERRELLAGFNPPRLSRPEELVHRLFSGNAARAADRPAVVC
                     DGHWISYSELEARSDRLAQRLRRRGVGPEAAVVLWLERSVEMVIALLAVLKAGGAYVP
                     LEVELPGERIASILACLRPPVVVTRRELAAALPEGTWDVLCLDEMALAETGEAGGPGE
                     APRSTVSVQNLAYVLFTSGSTGVPKGVSVTHGSLLNYLYGLWERLPPADGCRYGMVST
                     FAADLGHTVLFTSLCTGGTLCVISAERVGDPEALADDLASTGGADCLKIVPSHLAALL
                     RSELAGSILPRQALVLGGDVLHWSLVERVRSLASCAVFNHYGPTETTVGVLTHRYGSE
                     PPSSVSVPLGRPFTNVQVYVLGPRLQPVPRGIAGELYVGGESVARGYLGLPEQTAARF
                     IPDPFGGAPGGRLYATGDLVRHLPEGGIEFLGRVDLQVKIRGFRVELGEIEVALRRHP
                     QLRQAVVAVRGDGRGEQRLVAYVVPSPGASPGTVELRAFLRRSLPEFMLPAAVVALRS
                     LPLTANGKLDRRALPEPEQTVDALSYVPPRTAAEAELARIWQEILGLEKVGVHDNFFD
                     LGGDSILSIQIISRAHRAGLHLTPKQIFQHQTIAALAAAADAAPAATVAARARTAGSG
                     PVALTPIQRWFFALEQPVPHHFNQALLLAAWQPLEPARLAAALPSLLREHEALRFRFL
                     PGGSSGWQQRTGPAEEAAVWTHVDLGALPGERGREALTAAAAQLQGSLDLTRGPLLRV
                     AWFDLAGEPGRLLLIAHHLVVDGVSWRILLEDLEIAYRQLGEGEPVRLMPEATPFQLW
                     SERLTELSRSPEIEAELPYWLDVESADLPPWPLDGPGGPNDYASAGLVTLELDPAETT
                     DLLQRVPRPYRTRVDEVLLTALALAVSRWSGKRRVRVEIEGHGREPLLDDVDLSRTVG
                     WFTTHFPVLLDVRGLATPGAALQGVKEQLRHVPRRGIGYGLLRDPGASEGAAKLRFNY
                     LGQLDRGLPQDSLFAAAAEPIGPMQSPDNRRDCALDLTAAVLGGRLTLHWAYAGGLVS
                     RTTVERLAGHFVEELRGLIAHCLSPEAGGYTPSDFSLAVLDQARLDAIATERGEIADL
                     YPLSPLQRGLLFQFLYDPRSGLYLQQLTCLLHGRLDRAAFQSAWRQVLERNAILRTSF
                     AWQGLEAPLQVVHAECDLAWEEHDWRGLPDAVQQERLAALIPQERDRGLDPAVAPLMR
                     IVVVATKEQEVRFLWTYHHLILDGWSLPILLREVFSSYAAALRGEALALPRTRPFRDY
                     IEWLLRQDSAASEAYWRQALAGFTAPTSLGADRRRLDPGAAEDVEHDEQDDPGLLLSP
                     VETESLRLLARRWRLTLNTLLQAGWALLLHRYSGEEDIVFGVTLSGRPSQLEEVDTMV
                     GLFINTLPLRTRVDDSQSLATWLADLQARQVELAQHQASSLAEVQSWSDMQRGQPFFD
                     SIVVFENYPVDRSLQALGSDLGLSDVRSPERAKYPIVLYLYTGNDARLQARVNYDHRL
                     FETTSILRRLAHLEVVWRSFADTPAACLGDLPLLTDAERYQLLREWNEPSRFPVAGAL
                     HEVFERRAAETPQACALILGVDEVSYGELNRRVNRLAHRLLRSAAVTGGRIALCLDRS
                     LEMVIGILAVLKTGAAYVPIDPDYPRERKTFLLADSAASLLLTQERWLADLSPVDAPV
                     LCIEEVREEDGESDENPGVVVPPESPAYVIYTSGSTGWPKGV"
BASE COUNT          980 a         2074 c         2030 g          743 t
ORIGIN      
        1 ggaccccctt gggccatccc gtggagcccg aggtatagat cacgtaggcc ggagactccg
       61 gtggcaccac gacgcccggg ttctcgtcgc tctccccgtc ctcttcgcgc acctcctcga
      121 tgcagaggac cggcgcgtcg accggcgaga gatcggcgag ccagcgctcc tgggtgagca
      181 ggagcgacgc ggcggagtcg gccagcagga acgtcttgcg ctcgcgcggg tagtcgggat
      241 cgatcggcac atacgccgct cccgtcttga gcaccgccag gatgccgatc accatctcaa
      301 gcgagcggtc gaggcagagc gcgatccgcc ctcccgtcac cgccgccgat cggagcagcc
      361 ggtgcgccag acggttgacg cgccggttga gctccccgta cgagacctcg tccaccccga
      421 gaatgagggc acaggcttgc ggcgtctccg ccgcccgccg ctcgaacacc tcgtgcaggg
      481 ccccggcgac agggaaacgg ctcggctcat tccactcgcg cagcaactgg tagcgctccg
      541 cgtcggtcaa cagcggcagg tccccgaggc aggctgcggg ggtgtccgcg aagctccgcc
      601 agacgacctc cagatgcgcg aggcggcgca ggatcgaggt cgtctcgaaa aggcggtggt
      661 cgtagttgac ccgggcctgc agccgcgcgt cgttccccgt gtagagatag aggacgatcg
      721 ggtacttggc ccgctcggga gagcgcacgt cggagagccc gagatcgctg cccaacgcct
      781 gcagagacct gtctaccgga tagttctcga acaccacgat gctgtcgaag aacggctgtc
      841 cccgctgcat gtcgctccag ctctggacct ccgccaggga gctggcctgg tgctgcgcga
      901 gctcgacctg gcgcgcctgc agatcggcga gccaggtggc cagcgattgg ctgtcgtcga
      961 cccgcgtgcg gagcggcagc gtgttgatga agaggccgac catggtgtcg acctcctcca
     1021 gttgggaggg acgcccggag agcgtgacgc cgaagacgat gtcctcttca ccgctgtagc
     1081 gatgcagcag cagggcccag cccgcctgta gcagagtgtt cagagtcaga cgccaacgac
     1141 gggccagcag gcgcagggac tcggtctcca ccggcgacag cagcaacccg gggtcgtcct
     1201 gctcgtcgtg ctcgacatcc tccgccgcgc cggggtcgag gcggcgccgg tccgcgccga
     1261 gagaggtggg cgcggtgaag ccggcgagag cctggcgcca gtaggcctcg gaggcggcgg
     1321 agtcctgccg cagcagccat tcgatgtagt cgcggaacgg gcgcgtacgc ggcagggcga
     1381 gggcctcgcc acgcaacgcc gcggcatagg acgaaaagac ctcgcgcaac aggatcggca
     1441 acgaccagcc gtccaggatc aggtggtgat aggtccacag gaagcgcacc tcctgctcct
     1501 tcgtcgccac gacgacgatc cgcatcaggg gcgcgacggc aggatcgagg ccgcggtcgc
     1561 gctcctgtgg gatcagcgcg gcgagccgct cctgctggac ggcgtccggc aggccccgcc
     1621 agtcgtgctc ctcccaggcc aggtcgcact cggcgtgcac cacctggagc ggcgcttcga
     1681 gcccctgcca ggcgaaggag gtgcgcagga tggcgttgcg ctccagcacc tgccgccagg
     1741 cgctctggaa tgccgcacgg tcgagccggc cgtggagcag gcaggtgagc tgctgcaggt
     1801 acagcccgga cctcgggtcg tagaggaact ggaagagaag accccgctgc aggggcgaaa
     1861 gaggatacag gtcggcgatc tctccccttt ccgtagcaat cgcatcgagg cgcgcctgat
     1921 ccagcaccgc gagcgagaag tccgacggcg tgtagccacc ggcctccggc gacaggcaat
     1981 gggcgatgag gccgcgcagt tcctcgacga agtgcccggc caggcgctcg accgtcgtgc
     2041 gcgacacgag gccgccggca taagcccagt ggagcgtcag ccggccgccc aggaccgcgg
     2101 cggtcagatc cagagcgcag tcccgccggt tgtcggggct ctgcatcggc ccgatcggct
     2161 cggccgcggc cgcgaagagc gagtcctgcg gcaaccctct gtcgagctgg cccaggtagt
     2221 tgaaccggag cttcgcggca ccttcgctgg cgccgggatc ccgcagcagg ccgtagccga
     2281 tgccgcgccg gggaacgtga cgcagctgct ccttgactcc ctgcagggcc gcgccgggag
     2341 tcgcgagacc gcgcacatcg agcagcacgg gaaaatgggt ggtgaaccag ccgacggtgc
     2401 gcgagaggtc gacgtcgtcg agcagcggct cccggccgtg cccctcgatc tcgacccgga
     2461 cacggcgctt gccgctccac cgggagaccg ccagagcgag agcggtcaac aacacctcgt
     2521 ccacccgggt ccggtagggg cgcggcaccc gctgcagcag gtctgtggtc tcggccgggt
     2581 ccagctccag ggtcacgaga cccgccgaag cgtagtcgtt ggggccgccg ggaccgtcga
     2641 gcggccaggg cgggaggtcg gcagactcga cgtccagcca gtaagggagc tccgcctcga
     2701 tctccggcga gcgagagagc tcggtaagcc gctcgctcca gagctggaag ggggtcgctt
     2761 ccggcatcag gcgcaccggc tccccctcgc cgagctggcg ataggcgatc tccaggtcct
     2821 cgaggaggat gcgccacgag acgccgtcga ccaccaggtg atgagcgatc agcagcaggc
     2881 ggccgggctc gccggccagg tcgaaccagg ccacccgcag cagcggtccc cgcgtcaggt
     2941 ccaggctccc ctggagctgg gcggcggcgg ccgtcagcgc ctctctcccg cgctccccgg
     3001 gaagggcgcc gaggtcgacg tgcgtccaga cggccgcctc ctccgccggg cccgtccgct
     3061 gctgccaacc ggagctcccc cccggcagaa agcggaagcg cagggcttcg tgctcgcgca
     3121 gcagggaggg gagcgccgcc gcgagccgcg ccggctccag cggctgccac gccgccagca
     3181 ggagggcttg attgaagtga tgcggcaccg gctgctcgag cgcgaagaac catcgctgga
     3241 tgggggtcaa cgcgaccggc cccgagccgg ccgtacgtgc cctggcagcg acggtggctg
     3301 cgggcgccgc gtccgccgcc gctgccagag cggcgatcgt ctggtgctgg aagatctgct
     3361 tcggcgtgag gtgcagcccc gcccgatgcg ccctggagat gatctggatg ctgaggatcg
     3421 agtcgccgcc gagatcgaag aagttgtcgt gtacaccgac cttctcgagc ccaaggatct
     3481 cctgccagat gcgcgcgagc tccgcctcgg ccgcggtgcg cggcggcacg tacgacagcg
     3541 cgtcgaccgt ctgctccggc tccggcagcg ctctgcggtc caacttgccg ttggcagtca
     3601 acggcagaga cctcagggcc accaccgcgg caggcaacat gaactccggc aagctccggc
     3661 gcaggaaggc gcgcagctcg accgttcccg gcgacgctcc cggcgacggc acgacatacg
     3721 cgacgagccg ctgctcgcca cggccgtctc cacggacggc gaccaccgcc tggcgcagct
     3781 gcggatgccg gcgcagcgcg acctcgatct ccccgagctc gacacggaag ccgcggatct
     3841 tcacctgcag atccacgcgg ccgagaaact cgatccctcc ttcgggcaga tggcggacga
     3901 ggtcgcccgt ggcatagagc cgcccgccgg gcgcaccgcc gaatggatcg ggaatgaacc
     3961 tcgccgccgt ctgctcgggc agcccgagat agccgcgggc cacgctttcg ccgccgacgt
     4021 acaactcgcc ggcgatcccc cgtggaacgg gctggagcct cgggcccagg acatagacct
     4081 ggacattggt gaacggccgg cccaggggta cggagaccga agagggcggc tcgctgccgt
     4141 accggtgggt cagcacgccg acggtggtct ccgttggacc atagtgattg aagacggcgc
     4201 aggaagcgag cgaccgcacc cgctcgacca ggctccagtg cagcacgtcg ccacccagca
     4261 ccagcgcctg gcgaggaaga atgctccccg cgagctcgga gcgcagcagt gccgcgaggt
     4321 gggacggcac gatcttcagg cagtccgcgc caccggtcga cgcgagatcg tcagccagcg
     4381 cctcgggatc tcccaccctc tccgcggaga tgacacacaa ggtcccgccg gtgcacagcg
     4441 aggtgaacag caccgtgtga ccgagatccg cggcgaaggt ggagaccatt ccatagcgac
     4501 atccatcggc cggcggcagc ctctcccaca gaccgtacag gtagttgagc agcgaaccgt
     4561 gggtaacgga cactcccttc ggtacaccgg tcgagcccga ggtaaagagg acataggcca
     4621 ggttctgcac cgagaccgtg ctgcgcggcg cctcccccgg tccgccggcc tcgccggtct
     4681 cagccagcgc catctcgtcc agacagagca catcccacgt cccctccggc agagcggccg
     4741 ccagctcccg tcgtgtcacc accacgggtg ggcgcaggca ggcgaggatg gaggcgatcc
     4801 tctcgccggg cagctccact tccaggggaa cgtaggcacc gccggccttg agcacggcca
     4861 gcagagcgat caccatctcg accgagcgtt cgagccagag gaccacggcg gcctcaggcc
     4921 ctactccgcg gcgccgcagc cgttgcgcca gccggtcgga gcgtgcctcg agctcggagt
     4981 agctgatcca atgcccgtcg cacaccaccg cgggacggtc cgcggcgcgc gcggcgttgc
     5041 cggagaacag ccggtggacg agctcctcgg gacgggaaag gcgcggcggg ttgaagccgg
     5101 cgaggagctc gcgccgctcg tcttccggca gcatctcgag ctcgtcgatc cgcgcctcgg
     5161 ggtcgcggat cgcggccgcg aggagcgtct cgaactgcgc ggcgaggatc tcggcatagc
     5221 gggtcgtcca gcgccccgag tcgatctgca gctccaggag aagagagccc gggccggccg
     5281 cgcaacagag cgcgagatcg aagcgctccg cgctcaggaa gcgctcgacc tggaggaacg
     5341 acagagggcc ggccgaacgg cggatctggc gctcttccca ggcgaagccg aaggggaaat
     5401 agggcgggcc gcccgcctcg tccggcggca acgcctcctc gatgcggaac gactcctgcc
     5461 actccgcccc ttcctgcagc gcaacgtcga cgcgctgcac cagcgccagg aaacgcagcc
     5521 cctcttcgag gccggaatgc accggatgac agagagccat ggggccgagc actccctgca
     5581 ggagctcgtg atcacgcccc ggcctcacgg cgccgaccac cacctcgcgc tcgcccgtga
     5641 tccggcccac gaacgccagc caggcagcga gcaggaacgc ctggcccggc gcaccgcagc
     5701 gtccggcggc ctcctccaac gcggccggca ccagctgccg gcgcaccgtc gagagctgca
     5761 cctcggcttt cacctcggta ccggcaccgg cgcgcgcttc gaacggcagc cgcaaccgcg
     5821 ccatcga
//
LOCUS       DASPJI010000701         7448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_502516, whole genome shotgun sequence.
ACCESSION   DASPJI010000701 DASPJI010000000
VERSION     DASPJI010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7448)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7448
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502516"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VEL74_19740"
     CDS             <1..1209
                     /locus_tag="VEL74_19740"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HYG64823.1"
                     /translation="EIIQSIHLSLPDTFLRYLPAEPKEPRGRPALRRLATHLTDPARG
                     HHLLAPPPGGGAGLPGAERPAQATGSVGGGQGGAHTKEPSRSGPPPADPPKWLDRLAP
                     STLRLLLFGGKGGVGKTTCAAAAALTLAAHRPHARVLLVSTDPAHSLADVLDTSVGDD
                     ERPIPNTSPNLRVRELDAVQSFTRWRDRNRGEVGGVLGALTGNTADGEALTRLLDSPP
                     PGVDELLALSYLLEAAAGKEEGEDAFGLVVVDTAPTGHALRLLEMPEAALAWDHALLS
                     LLLKYRQALGLGDFAAGLVDLSKSLKRFRTLLSDPERSRFVAVTRRGELPRRETERLL
                     RKLARLSIPAPTVLLNAVPETPCPWCGPPAGSRPPAGGCDIMTAPAVFPPPRGAGVLA
                     AWARTWKETG"
     gene            1206..2000
                     /locus_tag="VEL74_19745"
     CDS             1206..2000
                     /locus_tag="VEL74_19745"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HYG64824.1"
                     /translation="MSRATYLYCLVRTQSGNPPPLDGVPPGLPGCAPPRLVGLAEAGD
                     GLWLVVADAPLPGYDGDEIQRHLQDLSWVSERALAHEAVVEHFLAAGTVIPMKLFTLF
                     AGDERAAAYVRGEGERLERVLARVDGCLEWGVQVRLDEARAREVVAVEARSGSGGQSA
                     GKGFLLRKKLEQEGARTLAERLRSEAGEVFEGLAEQAAEAIRREPPSPEAARRMLLDG
                     AFLVPRERSAAFESEVARAAERLAGRACELTLTGPWPPYNFLEEGR"
     gene            1997..2233
                     /locus_tag="VEL74_19750"
     CDS             1997..2233
                     /locus_tag="VEL74_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HYG64825.1"
                     /translation="MSPREDGEEGQAALELFEGGEGSLLDLADHLLNQGVVLTGDVVI
                     GLANVDLIYLRLSVLLCAADRVLPQAETPETTKP"
     gene            2230..2949
                     /locus_tag="VEL74_19755"
     CDS             2230..2949
                     /locus_tag="VEL74_19755"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HYG64826.1"
                     /translation="MSPLYVYALMAGEPEEPLGAGLAGEPLQLLSSAGLRVVAGDMAA
                     RPAPAPAALEAHDAVVRRLAERAAAVLPVRFGEWVEGEEALAERLAPRAASLAEALAL
                     VRGCVQMTLRVFGEPSQVEFQDPADRSDRSDLSEPAPGPGTRYLAARRREFERSRSLP
                     EIEPLRKALQPLLRAERVRRQETGRLVGTAYHLVPREAAGAYLGTLQAVGGTEGRLPA
                     GHRVAASGPWPPYAFGPEGLP"
     gene            2946..3293
                     /locus_tag="VEL74_19760"
     CDS             2946..3293
                     /locus_tag="VEL74_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013066801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein K"
                     /protein_id="HYG64827.1"
                     /translation="MSTREVVQLAVEEVEALRAELERHASAEAPRWNADPEEVQRSVA
                     RLVLALVEFLRRLLERQAIRRMEADTLTPEEVENIGVALMRLEETLHEIAARFGLDPE
                     ELNLDLGPLGRLI"
     gene            complement(3392..5794)
                     /locus_tag="VEL74_19765"
     CDS             complement(3392..5794)
                     /locus_tag="VEL74_19765"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HYG64828.1"
                     /translation="MRRSAATLPLLPLLLALAWLAVLLAPLLVPGRALANRDVPVFHL
                     PLRLTFAQLAGGAGALPSWNPFLHGGQPILSNPNYAAFYPLSWVALPVPAPYGLTLLV
                     VLHAGIAFAGGWFLARRLGCGTGAAALAGVGYAGSGAFLSLLSSFNFFCGMAWFPWVA
                     AWGSEALATGEESRRWRSALLAGGALALQLLAGEPAAAVISGLALLALAAAPLLARGS
                     GAPARRGTALRLAAVLLVALLLAAAQLLPTLRRLPDSPRSGGLDARAGTSWSAPPARL
                     AEVAFPRFFGDPARNQEGLFFGWKLHDGDYPYVPSLYPGLLLVVAGLAGLLRGPVPRR
                     SAWVLAVIAGAFLALGRHNPLYEAMREAVPMLAVLRFPEKFAVLSSAALGFAGILGWQ
                     RLLDERGDRRERADLPLALAALFLLMALALTGLLYLRPDAARGFIAEHGPPGLTPAAR
                     EAGLAFLRREGWTAVGMAAAVTGLLALCRSRRPSRRTLEVLAVGLLAADLWHYGHGLV
                     DTVPVEIYREPPPLARLPRPHGGRLFVEPPRGDEPRAVLRGGDPRSAVTRAALARLTP
                     YAGLLWQIPYALHEDYDLMLTGWARRALTTLHEEWEEPERAFRYMGVWNVGTALLHKE
                     PGAWAAELARDPTAPPMQAVPNPFLLPRFRFVPRASFHPTWEAALQEARAQGWLVHRH
                     EHAVRPGAPTAPVTYAGRPQTVEFTDERQRMRMRYTSEAPALFTAAVTFDGGWRAAVD
                     GAAVPVWPTAAGQMSVEVPAGEHEIVLEYHQPLLLRGVAISLTALAALAAALLRLRRQ
                     AT"
     gene            complement(5828..6376)
                     /locus_tag="VEL74_19770"
     CDS             complement(5828..6376)
                     /locus_tag="VEL74_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYG64829.1"
                     /translation="MPRIGNARQGFTLIELLIVIAIIGLIAAMLIPNLLDAMNKAKQK
                     KTIADMRITGTAMFSWLTDQVGAAAAGSESSVKIADYNPGKKDADQIKALMVPQYMQE
                     VPPVDGWKYPYEYYLRNDSPLSRQVMLIRSLGQDRQADRADYAVSAFEPTDYAQDIVW
                     ADGFFVRWPEKSGVEAPAPATP"
     gene            complement(6548..7264)
                     /locus_tag="VEL74_19775"
     CDS             complement(6548..7264)
                     /locus_tag="VEL74_19775"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYG64830.1"
                     /translation="MTTTGRGGHGSGLNPESANHQLVLGLARLLELPPRWRVSAPAGA
                     YLQALAPYQNEHWRRVYSRIEQIVTPGGPSEFLPPGLAGLFLDTIQVTVLRGGGRINV
                     IPGQAAASIDIRMLPDTDGDELLETIRRTLGNGFRVRVLVTSPSADPSPASGRFYDAV
                     QRTLAETDAPVVPALVSGFTDSRFFRERGIPAYGLSPFALGPDDLRGIHNPDERIPLA
                     ELDLGRERMIRIVEAYAAPR"
     gene            complement(7261..>7448)
                     /locus_tag="VEL74_19780"
     CDS             complement(7261..>7448)
                     /locus_tag="VEL74_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HYG64831.1"
                     /translation="AVADEENGGAQGTAWLLEHHPEIFRGVEAVAGEGGRNEVAPDGR
                     TLSWGIEVAQKRPLWLG"
BASE COUNT         1009 a         2677 c         2826 g          936 t
ORIGIN      
        1 gagatcatcc aatccatcca cctctccctc cccgacacct tcctccgcta cctgcccgcc
       61 gagcccaagg agcctcgagg ccgccccgcc ctgcgccgcc tcgccaccca tctcaccgat
      121 cccgcccgcg gccaccacct cctcgcaccc ccaccgggag ggggggcggg tctgccgggg
      181 gcggagcggc cggcgcaggc gactggcagc gtgggagggg ggcagggcgg agcgcacacg
      241 aaggagccga gcaggtccgg cccgcccccg gcagacccgc cgaagtggct ggaccgcctc
      301 gcaccctcca ccctccgtct cctccttttc ggcggcaagg gaggggtcgg caagaccacc
      361 tgcgccgccg ccgccgccct gactctcgcc gcgcaccgcc cgcatgcccg cgtcctgctg
      421 gtctccaccg accccgccca ctccctggcc gacgtgctcg acacctccgt gggagacgac
      481 gagcgcccca tccccaacac ctcgcccaac ctccgggtcc gggagctcga cgcggtccaa
      541 tccttcaccc gctggcgcga ccgcaaccga ggggaggtgg gaggggtcct gggagccctc
      601 accggcaaca ccgcggacgg ggaggcgctg acccggctcc tcgactctcc cccgccgggt
      661 gtcgacgagc tgctcgcgct ctcctacctg ctggaggcgg ccgccggcaa ggaggagggg
      721 gaggacgcct tcggcctcgt ggtggtggac accgcgccca ccgggcacgc gctgcgcctc
      781 ctggagatgc cggaggccgc cctggcctgg gaccacgccc tcctgtcgct gctcctcaag
      841 taccggcagg ccctggggct gggcgacttc gctgccgggt tggtggacct gtcgaagagc
      901 ctcaagcgtt tccggaccct cctctccgac ccggagcgct cccgcttcgt ggccgtcaca
      961 cgccgcggcg agctcccccg gcgggagacg gagcggctgc tccggaagct cgcgcggctc
     1021 tccatccccg cgcccacggt gctgctcaac gccgtgccgg agaccccatg cccctggtgc
     1081 gggccgccgg ccgggagccg gcccccggcc ggcgggtgcg atatcatgac cgcccccgcc
     1141 gtgttccccc cgccccgcgg cgccggcgtc ctggccgcgt gggcccggac ctggaaggaa
     1201 acgggatgag ccgagcgacg tatctctact gcctcgtccg gacccagagc ggcaacccgc
     1261 cgcccctgga cggagtcccc ccgggactgc ccggctgcgc gccaccgcgt ctcgtcggcc
     1321 tcgcggaggc gggcgacggg ctctggctcg tggtggccga cgcccccctg cccggctatg
     1381 acggcgacga gatccagcgc cacctccagg atctctcctg ggtctccgag cgcgccctgg
     1441 cccacgaggc cgtggtggag cacttcctgg ccgccggcac ggtgattccg atgaagctct
     1501 tcaccctctt cgccggcgac gagcgcgccg ccgcctacgt gcggggcgag ggcgaacggc
     1561 tggagcgggt gctggcacgc gtcgacggct gcctcgaatg gggggtgcag gtgcggctcg
     1621 acgaggcccg cgcccgcgag gtggtggccg tcgaggcgcg gagcgggtcc ggcggacaga
     1681 gcgccggcaa gggcttcctg ctgcgcaaga agttggagca ggagggggcc cggaccctcg
     1741 ccgagcggct gcggtccgag gccggggagg tcttcgaggg actggcggag caagccgcgg
     1801 aggccatccg gcgcgagccc ccttcgcccg aggcggcccg ccgcatgctg ctggacggcg
     1861 cgttcctggt cccgcgggag cggagcgccg cgttcgagag cgaggtggcg cgcgccgccg
     1921 agcgcctcgc cggccgcgcc tgtgagctca ccctcaccgg cccctggccc ccctacaact
     1981 tcctggagga ggggcgatga gcccccgcga ggacggcgag gaggggcagg ccgccctgga
     2041 gttgttcgaa gggggggagg gctctctcct cgatctggcg gaccacctgc tgaaccaggg
     2101 ggtggtgctc accggcgacg tggtcatcgg cctcgccaac gtcgacctga tctacctgcg
     2161 gctgtcggtg ctcctctgcg ccgccgaccg cgtccttccc caggcggaga cgccggagac
     2221 caccaagcct tgagccccct ctatgtctat gccctgatgg ccggcgagcc cgaggaacct
     2281 ctcggggccg gcctggccgg cgagcccctg cagctcctct ccagcgccgg attgcgcgtt
     2341 gtggcgggcg atatggccgc gcgtcccgcg ccggctcccg cggccctgga agcccatgat
     2401 gccgtggtgc ggcgcctggc cgagcgggcc gccgccgtcc tgccggtccg cttcggggag
     2461 tgggtggagg gggaggaggc cctcgccgag cggctcgctc cgcgggccgc gtcgctcgcc
     2521 gaggcgctgg cgctggtccg cgggtgcgtc cagatgacgc tccgggtctt cggcgagccg
     2581 tcgcaggtgg agtttcagga tccggcggat cggtcggacc ggtcggatct ctcggagccg
     2641 gctccggggc cgggcacccg ttacctggcc gcgcggcggc gcgagttcga gcggtcgcgc
     2701 tcgctccccg agatcgagcc gttgcggaag gctctgcagc cgctgctgcg ggcggagcgg
     2761 gtgcggcgcc aggagaccgg ccggctcgtg gggaccgcct accacttggt gccacgcgag
     2821 gccgccggcg cctacctggg gacgctccag gcggtcggcg ggacggaggg gcgcctgccc
     2881 gccggccacc gggtggcggc gagcgggccg tggccgccgt acgccttcgg gccggagggg
     2941 ctgccgtgag cacgcgtgag gtggtccagc tcgccgtcga ggaggtcgag gcgctccgtg
     3001 ccgagctgga gcgtcacgca tcggccgagg cgccgcgctg gaacgccgat ccggaggagg
     3061 tgcagcgctc ggtcgcccgg ctcgtgctgg cgctggtgga gttcctccgc aggctgctgg
     3121 agcggcaggc gatccggcgc atggaggccg acaccctgac ccccgaagag gtcgagaaca
     3181 tcggcgtcgc cctcatgcgc ctggaggaga cgctgcacga gatcgccgcg cgcttcggcc
     3241 tcgatcccga ggagctgaac ctcgatctgg gaccgctcgg ccgtctgatc tagcggccgt
     3301 tcttccggga tggggtccat tccggagcga gctgccgccg ttcggcgggg aatggccatc
     3361 gttcccgtgg gaatgacggc cattcctctg ctcaggtcgc ctggcggcgg agccgcagga
     3421 gcgccgccgc cagcgccgcg agggcggtca ggctgatggc cactccccgg aggaggagcg
     3481 gctggtggta ctccagcacg atctcgtgct cgccggccgg cacctcgacg ctcatctgcc
     3541 ccgccgctgt cggccagacc gggaccgccg ccccatccac cgccgcccgc cagccgccgt
     3601 cgaaggtgac cgccgcggtg aagagcgccg gcgcctccga ggtgtagcgc atccgcatcc
     3661 gctggcgctc gtcggtgaac tccacggtct gtggccggcc ggcgtaggtc acgggagccg
     3721 tgggggcgcc cggacggacg gcgtgctcgt gccggtggac gagccagccc tgggcgcgcg
     3781 cctcctgcag cgccgcctcc caggtgggat ggaagctcgc ccgcggcacg aaccggaagc
     3841 gcgggaggag gaaggggttg ggcacggcct gcatcggcgg cgccgtcggg tcgcgcgcca
     3901 gctcggcggc ccacgcgccg ggctccttgt ggaggagcgc cgtgccgacg ttccacaccc
     3961 ccatgtagcg gaacgccctc tctggctcct cccactcctc gtgcagcgtg gtgagggccc
     4021 gccgcgccca gccggtgagc atcaggtcgt agtcctcgtg cagggcatag gggatctgcc
     4081 agaggagccc cgcgtagggg gtcagccggg cgagggccgc ccgggtcacg gcgcttctcg
     4141 ggtcccctcc gcgcaggacg gcccggggct cgtcgccacg cggcggctcg acgaacagcc
     4201 ggccgccgtg cgggcgcggc agccgtgcga gagggggcgg ctcgcgatag atctccacgg
     4261 gaaccgtgtc caccagccca tggccgtagt gccagaggtc cgcggcgagc aggccgacgg
     4321 cgaggacctc gagcgtgcgg cgggaggggc ggcgggaacg gcagagggcc aggaggccgg
     4381 tcaccgccgc cgccatgccc acggccgtcc atccctcccg ccgcaggaag gccaggcccg
     4441 cctcccgggc ggccggagtg aggcccggcg ggccgtgctc ggcgatgaac ccccgcgccg
     4501 cgtccggcct gagatagagg aggccggtga gggcgagcgc catgagcagg aacagcgccg
     4561 ccagggccag ggggaggtcg gcgcgctcac ggcgatcacc gcgctcgtcc aggagccgct
     4621 gccagccgag gatcccggcg aaccccaggg cggcgctgct cagcaccgcg aacttctcgg
     4681 ggaagcggag caccgcgagc atcgggaccg cctcccgcat cgcctcgtag agcgggttgt
     4741 ggcgcccgag cgccaggaag gcgccggcga tgacggcgag cacccaggcg gaccgccgcg
     4801 gcaccgggcc ccgcagcaga cccgcgaggc cggccaccac caggagcagt cccggataga
     4861 gggaggggac gtagggatag tcgccgtcgt ggagcttcca gccgaagaac agcccctcct
     4921 ggttgcgcgc cggatcgccg aagaagcgcg ggaaggccac ctcggccaag cgcgccggcg
     4981 gcgccgacca ggaggtgccc gcgcgggcgt ccagcccgcc gctccgcggg gagtcgggca
     5041 ggcgccgcag cgtcggcagg agctgggccg cggcgagcag gagggccacg aggaggaccg
     5101 cggctaggcg cagggcggtc ccacggcggg ccggggcgcc ggagccgcgg gcgagcaggg
     5161 gcgccgcggc gagggccagc agtgcgaggc cgctgatcac ggcggcggcg ggctcgccgg
     5221 ccagcaactg gagagcgagc gcgccgccgg cgaggagcgc cgagcgccag cggcggctct
     5281 cctcgcccgt cgcgagggcc tccgaccccc aggccgcgac ccaggggaac caggccatgc
     5341 cgcagaagaa gttgaagctg ctgaggagcg acaggaacgc gccgctgccg gcatagccga
     5401 ctccggccag cgccgccgcg cccgtgccgc agcccagccg ccgcgccagg aaccagccac
     5461 cggcgaaggc gatgccggcg tggagcacca ccaggagcgt cagcccatag ggggcgggga
     5521 ccggcagcgc cacccaggac agcgggtaga aggcggcgta gttcgggttc gagaggatcg
     5581 gctggccgcc gtgcaggaac gggttccagg agggcagggc gccggcgcct ccggcgagct
     5641 gtgcgaaggt gagccgcagc ggcagatgga agaccgggac gtcccggttg gccagcgccc
     5701 gccccggtac gaggagcggg gccagcagga cggcgagcca ggccagagcc aggaggagcg
     5761 gcaggagcgg gagggtcgcg gcggaacggc gcatgcggcg gcggattgta ccgccgcgac
     5821 ctcctctcta cggagtcgcc ggagcggggg cttcgacgcc gctcttctcg ggccagcgga
     5881 cgaagaagcc gtccgcccag acgatgtcct gggcgtagtc ggtcggctcg aaggccgaca
     5941 ccgcgtagtc cgcccggtcg gcctgccggt cctggcccag gctgcggatc agcatcacct
     6001 gccgcgacaa cggcgagtcg ttgcggaggt agtactcgta cgggtacttc cagccgtcga
     6061 ccggtggcac ctcctgcatg tactgcggca ccatcagcgc cttgatctgg tcggcgtcct
     6121 tcttgcccgg gttgtagtcc gcgatcttga cggagctctc cgagcccgcc gccgcggctc
     6181 cgacctgatc ggtcaaccag gagaacatcg ccgtgccggt aatgcgcatg tcggcgatgg
     6241 tcttcttctg tttggccttg ttcatggcat cgagcaggtt cgggatcagc atcgcggcga
     6301 tgaggccgat gatggcgatc acgatcagca gctcgatgag cgtgaagccc tgccgtgcgt
     6361 tcccgatccg aggcatccct cgcattggaa aaatcccccc tttggacctg attgaactgc
     6421 ttacatctcc agtctagagc aaggccggtg ccagctttgg cttttcggta acaacaatga
     6481 aatcaacagt ttggctggag agggggcgcg ccgctgacaa aaagggtcag ccgagcggca
     6541 aaggctgtca ccggggggcg gcgtaggcct cgacgatccg gatcatgcgc tcgcggccga
     6601 ggtcgagctc ggccaggggg atgcgctcgt ccgggttgtg gatgccgcgg aggtcgtccg
     6661 gtccgagggc gaagggggag aggccgtagg cggggatgcc gcgctcccgg aagaagcgcg
     6721 agtcggtgaa gccggagacc agggcgggga ccaccggcgc gtcggtctcc gcgagcgtcc
     6781 gctggacggc gtcgtagaag cggccggagg cgggggaggg atcggccgac ggcgaggtga
     6841 cgaggacccg cacccggaag ccgttcccga gggtgcggcg gatggtctcc agtagctcgt
     6901 cgccgtcggt gtcgggcagc atgcggatgt cgatgctggc ggcggcctgg ccggggatga
     6961 cgttgatccg gccgccgccc ctcagcaccg tcacctggat cgtgtcgagg aagaggccgg
     7021 ccaggcccgg cggcaggaac tcgctcgggc cgccgggggt gacgatctgt tcgatccgcg
     7081 agtagacgcg gcgccagtgc tcgttctggt acggcgccag cgcctgcagg taagcgccgg
     7141 ccggcgccga cacccgccag cgggggggca gctccagcag gcgggcgagc ccgaggacga
     7201 gctggtggtt ggcgctctcc gggttgaggc cggagccgtg gccgccccgc ccggtggtcg
     7261 tcaccccagc cagagcggcc gcttctgcgc cacctcgatc ccccaggaga gggtccggcc
     7321 gtcgggggcg acctcgttgc gtccgccctc accggccacc gcctcgacgc cccggaagat
     7381 ctccgggtgg tgctcgagga gccaggcggt gccctgggcc ccgccgttct cctcgtcggc
     7441 cacggcga
//
LOCUS       DASPJI010000702         3123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_850254, whole genome shotgun sequence.
ACCESSION   DASPJI010000702 DASPJI010000000
VERSION     DASPJI010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850254"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VEL74_19785"
     CDS             complement(<1..1078)
                     /locus_tag="VEL74_19785"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HYG64832.1"
                     /translation="MILTSEQVDDLSRLQEGTFPQVAFPALLFSFALRKRTLVLEIRR
                     RQLWKKIVLEEGVPVDCRSNLAHETLGRYMVAEGKLSEEDFSICLSRSAAHGVPLGEV
                     LLEQGLVSAVELFKILQANLAQKLLDVFTWRDGEFRTLAELPPLDSSLKVKVPQLIVT
                     GVTKCSPQEEVDMAVGPLVGKKLALHPTPPFPLEDIKLNQRQAQLVEALRAGRRLGEL
                     AEASGMPLDEITRVIYALSTIGAVGPADALPRLPTGAMPTTGTVATAAAAPTAAPARP
                     AMSATMSIPAVSPEAERRRNEVMQAYLSYRRQDSFDLLGVPEEAPFGTIQEKYLDFAR
                     RFNPWTGGADLTGMEEKVQDLFLAG"
     gene            1169..1795
                     /locus_tag="VEL74_19790"
     CDS             1169..1795
                     /locus_tag="VEL74_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HYG64833.1"
                     /translation="MLFDGPEGGGLTLALAHGAGAPMDSPFMNTVAHGLAAEGVRVAR
                     FEFPYMRARREPASGSGRRGGAPDREPVLRQAWLAVVEELGGGGRVVVGGKSMGGRIA
                     SMIADECGARGVVCLGYPFHPPGQLEKLRTAHLEHLRTPALIVQGTRDPFGTREEVAG
                     YALSSGIRLHWLEDGDHSFKPRVSSGHTEAQHLREAMAAVKSFLGSLA"
     gene            1797..2714
                     /locus_tag="VEL74_19795"
     CDS             1797..2714
                     /locus_tag="VEL74_19795"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYG64834.1"
                     /translation="MADFSSSFSPYAGQLAALGAASGWAVSTLAFETASRRIGSMPAN
                     LVRLPIAFAFLTLAAWLARGNPLPADASPHAWIWLGVSGLAGFSFGDLCLYRAYLLIG
                     PRLSSLMMSLVPPLAALIGWAVLGETLSGRDLLGMGLTLGGIVWAILERQPRGSAASA
                     GHPPMTGLALGFGGALGQAVGLILSKLGMGSYHPLAANQIRVIAGFLGFAAIFTVLGI
                     WPRVGSALRDTRALGFATLGAFFGPFVAVSLSLVAIRATTAGVAASIMATTPILVIPL
                     VILLRGERVGLGGLGGAVLAVAGVALLFL"
     gene            2822..>3123
                     /locus_tag="VEL74_19800"
     CDS             2822..>3123
                     /locus_tag="VEL74_19800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64835.1"
                     /translation="MKFDRSLWLAGAATFALLPVLAAPAFAGPRPVGPEFRVNLTSEF
                     KQLNPQAAYNNAGQALVVWENPQHGIRGIYYNSNGQPVGAEMSLVPNQTLGAIPARG"
BASE COUNT          395 a         1080 c         1146 g          502 t
ORIGIN      
        1 cgccggccag gaacaggtcc tggaccttct cctccatgcc ggtcaggtcc gcccccccgg
       61 tccacgggtt gaagcggcgg gcgaagtcca ggtacttctc ctggatcgtg ccgaaggggg
      121 cctcctcggg aacccccagc aggtcgaagg agtcctgccg gcgataggag aggtaggcct
      181 gcatcacctc gttgcgccgg cgctcggcct cgggggagac ggcggggatc gacatggtcg
      241 ccgacatcgc cggccgggcc ggtgccgccg tgggggcggc ggcggccgtc gccactgtcc
      301 ccgtcgtggg catggcgccg gtgggcaggc ggggcagcgc gtccgcggga ccgaccgcgc
      361 cgatcgtgga cagcgcgtag atcacccggg tgatctcgtc caggggcatg ccgctcgcct
      421 ccgccagctc acccagccgg cggccggcgc gcagggcctc gacgagctgg gcctggcgct
      481 ggttgagctt gatatcctcc agcgggaagg ggggcgtggg gtggagggcg agcttcttgc
      541 ccaccagcgg ccccacggcc atgtccacct cttcctgggg ggagcacttg gtgacaccgg
      601 tgacgatgag ctgcggcacc ttgaccttga gggaggagtc caggggcggg agctcggcca
      661 gggtccggaa ctcgccgtcg cgccaggtga agacgtccag cagcttctgg gcgaggttcg
      721 cctgcaggat cttgaacagc tcgacggcgc tcaccagccc ctgctcgagg agcacctcgc
      781 ccagggggac gccgtgcgcc gctgagcggg agaggcagat gctgaaatcc tcctcggaga
      841 gcttgccttc ggcgaccatg taccggccga gcgtctcgtg cgccaggttg gaccggcagt
      901 cgacaggcac gccctcttcg aggacgatct tcttccagag ctggcggcgg cggatctcca
      961 gcaccagcgt ccgcttgcgg agggcgaacg agaacagcag ggccgggaag gcgacctgtg
     1021 ggaaggtccc ctcctgcaag cgggagagat cgtcgacctg ctcggacgta agaatcacgc
     1081 ggcagccttt cgcgcgcgag gcaatcgctc agagaaaaac tgccatggag gctaaccggt
     1141 gagcccggat gtgtcaatga ggcccgagat gttgttcgac ggtcccgagg ggggcggcct
     1201 cacgctggcc ctcgcgcacg gcgccggggc gccgatggac tcccccttca tgaacaccgt
     1261 ggcccatggc ctggcggcgg agggggtgcg cgtggcgcgc ttcgagttcc cctacatgcg
     1321 ggcgcgaagg gagcctgcct ccggcagcgg gcgccgcggc ggcgcgccgg atcgcgagcc
     1381 cgtgctgcgc caggcctggc tcgccgtggt ggaggagctg gggggcggcg ggcgggtggt
     1441 ggtcggcggc aagtcgatgg gcggccggat cgccagcatg atcgccgatg agtgcggcgc
     1501 gcgcggcgtg gtctgcctgg gctacccgtt ccatccgccg gggcagctcg agaagctgcg
     1561 caccgcccat ctggagcacc tccgcacccc ggcgctcatc gtccagggga cccgcgaccc
     1621 gttcggcacg cgggaggagg tcgcgggcta cgcgctgtcg tcgggcatcc gcctccactg
     1681 gctggaggac ggcgaccact cgttcaagcc gcgggtctcc tccggccaca ccgaggcgca
     1741 gcacctccgc gaggcgatgg cggcggtgaa gtccttcctg ggctccctgg cctgacgtgg
     1801 ccgacttctc ttcatccttc tctccctatg cgggccagct cgcggccctc ggcgccgcga
     1861 gcggctgggc cgtgtccacc ctggcgttcg agacggcgtc gcggcggatc gggtcgatgc
     1921 cggcgaactt ggtgcggctg cccatcgcct tcgccttcct gacgctggcg gcctggctgg
     1981 cgcggggcaa ccccttgccg gcggacgcct cgccgcacgc ctggatctgg ctgggcgtct
     2041 ccggcctggc cggcttctcg ttcggcgacc tctgcctcta ccgcgcctac ctgctcatcg
     2101 ggccgcggct gtcgagcctg atgatgtcgc tcgtcccccc gctggccgcg ctgatcggct
     2161 gggccgtgct gggggagacg ctctcggggc gggacctgct cggcatgggc ctcaccctgg
     2221 gagggatcgt ctgggcgatc ctggagcgcc agccccgcgg ctcggcggcg tccgccgggc
     2281 accctcccat gaccggcctc gccctgggat tcggcggcgc cctggggcag gcggtgggtc
     2341 tgatcctcag caagctcggg atggggagct atcacccgct ggcggccaac cagatccggg
     2401 tgatcgccgg cttcctgggc tttgccgcca tcttcaccgt cctggggatc tggccgcgcg
     2461 tggggtcggc gctccgcgac acccgggccc tgggcttcgc caccctgggg gccttcttcg
     2521 gcccgttcgt cgccgtatcg ctgtcgctgg tggcgatccg cgccaccacg gccggggtgg
     2581 cggccagcat catggccacg acgccgatcc tcgtcatccc gctggtgatc ctgctgcgcg
     2641 gcgagcgggt ggggctcggc ggcctgggcg gggcggtgtt ggcggtggcc ggggtggcgc
     2701 tgctgttcct ctgagccatg cttctatacc attgactccc aaccggctcc gggcctagga
     2761 tggaggggtt tccacagcgc actctgtgct cgttcgcacg ttcattcaag gaggtcgagg
     2821 aatgaagttt gatcgctccc tctggctggc tggggccgcg acattcgcgc tgctccccgt
     2881 gctggccgct ccggcctttg ccggcccccg gccggtcggc ccggagttcc gtgtcaatct
     2941 gaccagcgag ttcaagcagc tcaacccgca ggcggcgtac aacaacgccg gccaggccct
     3001 ggtggtctgg gagaaccccc agcacgggat ccgcggcatc tattacaact ccaacggcca
     3061 gccggtgggc gccgagatgt ccctggtgcc gaaccagacg ctgggcgcga tcccggcccg
     3121 tgg
//
LOCUS       DASPJI010000703         4033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_215260, whole genome shotgun sequence.
ACCESSION   DASPJI010000703 DASPJI010000000
VERSION     DASPJI010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215260"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1405)
                     /locus_tag="VEL74_19805"
     CDS             complement(131..1405)
                     /locus_tag="VEL74_19805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64836.1"
                     /translation="MEPVPSRLRNWVKILSAYFTAQTLTQLAGIAAGLLFINFMPVRD
                     FALYTLASSVITFFTFASDLGSTTSLVHFFHRSAREGEDFGSYVAAVLSLRRWAFLLG
                     SLAVLAVFPRIAMERGFGAVETALAAAAVAVGVWFQIGASIRVLLLRLADRYGLSYRA
                     ELAGAAFRLLAAGAMIAAGLLYGWLGVAAAAAGTAVTAWLARQGAAVPESKTDAAESR
                     RRVLRYLLPTLPSALYFAVQGPLVVWLAATFGSARNIAEVGALGRLGLVVGIFSGLTG
                     VVFLPRLARITDDRLYRARYFQFGALLATVALGMLAAAALFPGLFLLLLGESYSGLHR
                     ELLLVVAAAGLTLLDGYAVAVNLARSWTRWQGGAVLSLALSQAALAALLPLSTTSGVL
                     LFNLLSSGVALAGQLAINAAGFLRPRWVQWAS"
     gene            complement(1405..3213)
                     /gene="asnB"
                     /locus_tag="VEL74_19810"
     CDS             complement(1405..3213)
                     /gene="asnB"
                     /locus_tag="VEL74_19810"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HYG64837.1"
                     /translation="MCGINGIVRLAEAAPPIDSGELIRTRDAMTARGPDGEGAWVSPC
                     GRAALASRRLAILDLSPAGAQPMASADGRFHIVLNGEIYNFLELRQELERTGERFRSR
                     SDTEVVLALYAREGTAMLPRLRGMFGLAIWDDATKTLLLARDPLGIKPLYYSTHGGTL
                     RFASQVKAIERGGAIPLDVDPAGLAGFLLWGSVPEPLTIRRAVRALPAGSYLKVEDGR
                     LSEPALFYSFPPPPLPGREEGRRERGPGGEGSALDDAVRAHLVSDVPVAVFLSAGLDS
                     SLIAALARRHLPEPPVTFTLSFDRLAGTPLDEAPLAAEVAKTLGTRHVERRVGREEFL
                     GLWSGAVAAMDQPSIDGFNTYLVSRTAHEAGLTVVLSGLGGDELFGSYPSFIDVPKGV
                     KAARWGRRLPGLASLWPAVAGRLRPDQPKMRGLLRYGGDLAGAYFLRRALFLPEELPA
                     LLGPDLAAEGLSGYDPVADVRKVATANGDPWLAVHAFETARYMRNQLLRDSDWASMAW
                     SVELRVPWVDARLREALAAAGFEPARSRGKAALVRQTAPELPAALFDRPKTGFYIPVM
                     EWLLPGETRVRNLGGQSRELAVRVLEEFGIPLGGRG"
     gene            complement(3217..>4033)
                     /locus_tag="VEL74_19815"
     CDS             complement(3217..>4033)
                     /locus_tag="VEL74_19815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYG64838.1"
                     /translation="DLLGPSRVQAYLPSRLRAPYLIPLYGIEVWRPLSWDRARALANA
                     TVRLAISTHTLERARPFCPGLDGTAVLPLALEERPPAGEVDGSLLERAGEGFLLIVGR
                     MASTERYKGHDQLLEAMPRLPGARLVIAGEGDDRPRLEEKARALGIASRVVFTGFVSE
                     ATLAGMYGRCAVFAMPSRGEGFGLVYLEAMRAGKPCVAARASAAEEVVADGGTGLLVD
                     PDDPGELTGALERLLENPDVARKMGEAGRRRWRSEFSVERFWSRLEPLLGRLV"
BASE COUNT          641 a         1498 c         1451 g          443 t
ORIGIN      
        1 aagctcacca gcttgacctc gtcgacctcc ggcaggcggg ccatggcgcg ggacagctcc
       61 tcggcgaagc gctcgccgcc gcccagcagg gattccggcg cgaagtaggt cgggctcacg
      121 tggcagacct tcatgacgcc cactgcaccc agcgcgggcg gaggaagccg gcggcgttga
      181 tggcgagctg cccggcgagg gcgacacccg agctcaggag gttgaacagc agcacgcccg
      241 aggtggtgga gagggggagc agtgccgcca gcgccgcctg ggagagggcc agggagagaa
      301 ccgctccccc ctgccagcgg gtccaggagc gggccaggtt caccgccacc gcgtagccgt
      361 ccaggagcgt cagccccgcg gctgccacca ccaggagcaa ctcgcggtgc agacccgagt
      421 agctctcccc gaggaggagc aggaacaggc ccgggaagag ggccgctgcc gccagcatcc
      481 ccagggcgac ggtggcgagc agggctccga actggaagta acgggcgcgg tacagccggt
      541 cgtccgtgat ccgcgccagg cgcggcagga agacgacgcc ggtgagcccg gagaagatcc
      601 ccaccaccag gccgagccgt ccgagcgccc cgacctcggc gatgttgcgg gccgagccga
      661 acgtcgccgc cagccagacc accagcggcc cctggaccgc gaagtagagg gcactgggca
      721 gcgtcggcag gaggtagcgc agcacccggc gccgggactc ggcggcgtcc gtcttactct
      781 ccggcacggc ggcgccctgc cgcgccagcc aggccgtcac cgccgtgccg gccgccgcgg
      841 cggcgacgcc cagccagccg tagaggagcc cggcggcgat catcgccccc gccgccagga
      901 gccggaaggc tgcccccgcc agctccgccc ggtaggagag cccgtagcgg tcggccaggc
      961 gcaggaggag cacccggatc gacgcgccga tctggaacca gaccccgacc gcgacggccg
     1021 ccgcggcgag ggcggtctcc accgcgccga agccgcgctc catggcgatc cgcgggaaca
     1081 ccgccagcac ggcgaggctt cccagcagga aggcccagcg gcgcagcgac agcacggcgg
     1141 ccacgtagct cccgaagtcc tccccctccc gcgccgagcg atggaagaag tggacgagcg
     1201 aggtggtgct ccccaggtcc gaggcgaagg tgaagaaggt gatcaccgac gacgccaggg
     1261 tgtagagggc gaagtcccgc accggcatga agttgatgaa cagcaggccg gccgcgatcc
     1321 ccgcgagctg ggtgagggtc tgcgcggtga agtaggcgga gaggatcttc acccaattcc
     1381 gcaggcgcga ggggaccggt tccatcagcc cctccctccg agggggatgc cgaattcctc
     1441 cagcacccgc accgccagct cccgcgactg cccgccgaga ttccggacgc gggtctcgcc
     1501 ggggaggagc cactccatca ccgggatgta gaagccggtc ttcggacggt cgaagagcgc
     1561 cgccggcagc tccggggcgg tctgccgcac cagcgccgcc ttcccccggc tgcgggcggg
     1621 ctcgaagccg gcggcggcga gggcctcgcg caggcgcgcg tccacccagg gcacccgcag
     1681 ctccaccgac caggccatgg aggcccagtc cgaatcccgc aggagctggt tccgcatgta
     1741 ccgggcggtc tcgaaggcat ggaccgccag ccagggatct ccgttcgcgg tcgccacctt
     1801 acggacgtcc gccaccgggt catagccgct cagcccctcc gcggcgaggt ccggtcccag
     1861 gagggcgggc agctcctcgg ggaggaacag ggcgcgccgc aggaagtagg cgccggccag
     1921 gtcgccgcca tagcgcagca atccccgcat cttgggctgg tccggccgca gccgtcccgc
     1981 cacagccggc cagagggacg cgagccccgg cagccgccgc ccccagcggg ccgccttcac
     2041 ccccttgggg acatcgataa aagatggata gctgccgaac agctcgtcgc cgcccaggcc
     2101 ggacagcacc accgtcagcc ccgcctcatg ggccgtgcgg ctcaccagat aggtgttgaa
     2161 cccgtcgatg ctcggctggt ccatggccgc gaccgcgccg gaccagaggc cgaggaactc
     2221 ctcccggccc acccgccgct ccacgtggcg ggtccccagc gtcttcgcca cctcggcggc
     2281 cagcggcgcc tcgtccagcg gcgtgccggc gaggcggtcg aagctcagag tgaaggtgac
     2341 cggcggctcg gggaggtggc gccgggcgag ggcggcgatg aggctcgagt cgagccccgc
     2401 cgagaggaag accgccaccg ggacgtccga gacgagatgg gcgcggacgg cgtcgtccag
     2461 ggcggagccc tctccccccg gccccctctc ccgcctcccc tcctccctcc ccgggagagg
     2521 gggaggcggg aaggagtaga agagggcggg ctcgctgaga cggccgtcct cgaccttcag
     2581 atagctgccg gcggggaggg ctcggacggc gcggcggatg gtgagcggct ccggcaccga
     2641 gccccagagg aggaagccgg cgaggccggc ggggtccacg tccaggggga tcgcgccgcc
     2701 cctctcgatc gccttcacct gcgaggcgaa gcggagggtt cctccgtgcg tcgagtagta
     2761 gagcggcttg atgcccagcg gatcgcgggc gaggaggagc gtcttcgtgg cgtcgtccca
     2821 gatggcgagc ccgaacatgc cgcgcaggcg ggggagcatg gccgtcccct cccgggcata
     2881 aagggcgagc accacctcgg tatcgctccg cgaccggaac cgctcgcccg tccgctccag
     2941 ctcctgccgc agctccagga aattgtagat ctcgccgttg agcacgatgt gaaagcgccc
     3001 gtccgccgaa gccatcggct gggcgcctgc gggcgagagg tcgaggatcg cgagccgccg
     3061 gctggccagg gccgcccggc cgcagggcga cacccacgcc ccttcaccat ccggcccgcg
     3121 agccgtcatg gcgtcccgcg ttcggatcag ctccccggag tcgatgggcg gagccgcctc
     3181 cgccaagcgc acgatgccgt tgatcccgca catgatctag accaaccgcc ccagaagcgg
     3241 ctccagccgg ctccagaagc gctcgacgga gaactcgctc cgccagcgcc gccggcctgc
     3301 ttcgcccatc ttccgggcga catccggatt ctccagcaga cgctccagcg ccccggtcag
     3361 ctccccggga tcgtccgggt ccaccagcag cccggtcccg ccgtccgcca ccacctcctc
     3421 ggcggcgctc gcccgggccg cgacgcacgg cttgccggcg cgcatcgcct ccaggtagac
     3481 gaggccgaac ccctcgccgc ggctgggcat ggcgaagacc gcgcaccggc cgtacatccc
     3541 ggccagggtg gcctcgctca cgaaaccggt gaagaccacc cgcgaggcga tccccagagc
     3601 gcgggccttc tcctccagcc gcggccggtc gtccccctcg ccggcgatca ccagcctggc
     3661 gccgggcagc cgcggcatgg cttcgaggag ctggtcgtgc cccttgtagc gctcggtgga
     3721 ggccatccgc ccgacgatga ggaggaaccc ctcgccggcc cgttccagaa ggctgccgtc
     3781 cacctccccc gcgggcggac gctcctccag ggcgaggggg agcacggcgg tcccgtccag
     3841 cccggggcag aaggggcgag cccgctccag ggtatgggtc gagatggcga ggcggaccgt
     3901 ggcgttcgcc agcgcgcggg cgcggtccca gctcagtggg cgccagactt cgatgccgta
     3961 gagcgggatc aggtacgggg cgcgcagccg ggaggggaga taggcctgca cccgggacgg
     4021 ccccagcaga tcg
//
LOCUS       DASPJI010000704         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_789862, whole genome shotgun sequence.
ACCESSION   DASPJI010000704 DASPJI010000000
VERSION     DASPJI010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789862"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VEL74_19820"
     CDS             <1..771
                     /locus_tag="VEL74_19820"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HYG64839.1"
                     /translation="EVGGQVFTDFLQIDASINPGNSGGPLVNLAGEVIGVNTAIIQGA
                     NGIGFAIPADRARRVVDELLRFGELRPVWTGARLVTVDPEMARRAGLPVPRGAAVLKV
                     YPDSPATAAGLREQDVIVGVNGRPAASREEVATALYTVTPGTPVRLDVRRGEQTLQLT
                     LRAVQPPERLGMELLRVGVGLVTEESRNGLVVQRVIAGSEAAERGLRPGDVVLGANGQ
                     RARTHDELSREVLRGIERGGLLLAVQRGRYVYNIGFPL"
     gene            852..1532
                     /locus_tag="VEL74_19825"
     CDS             852..1532
                     /locus_tag="VEL74_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64840.1"
                     /translation="MSQSKLAAPQLVINSRMIYSSWIPADPKAAAALLPDRLTPAANG
                     AIYMNQYVVDSEEQTSGFGAYSLTYMGLDLGNVTAPDGVTPGRFFTNYFNSSEIMREY
                     VRERGVPATPGTTTVEVHGHELEATTFVDGVAIIRTRARVGKKVGVVARGHLTYVTQL
                     DGKLIAGNYAYIGELVDPFELQSVEFLTKDHPAWALRPAQPLQVVTGSSWFAPRDSFV
                     YPGGEREI"
     gene            complement(1541..>2223)
                     /locus_tag="VEL74_19830"
     CDS             complement(1541..>2223)
                     /locus_tag="VEL74_19830"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HYG64841.1"
                     /translation="RWWHPAWGLVPPDQFIHVAEETGLILPIGRWVLTEACEALHRWQ
                     QEYPSDPPLAINVNLSRRQLLQTDLIEQIRLILHRTGLPAPSVRLEITESSILENPEA
                     AADLLRHLKALEIGLCVDDFGTGYSSLSSLQQFPVNILKIDRSFIRGMGPDGEKDEIV
                     RAVVGLAHSLRMKVVAEGVETEGQLSRLKAMHCDYGQGFLLCQALDPEGVERLLETSG
                     RAPVGLPS"
BASE COUNT          340 a          759 c          791 g          333 t
ORIGIN      
        1 gaggtcggcg ggcaggtgtt caccgacttc ctgcagatcg acgcctcgat caaccccggc
       61 aactcggggg gcccgctggt gaacctcgcc ggcgaggtga tcggcgtcaa caccgcgatc
      121 atccaggggg cgaacggcat cggcttcgcc atccccgccg accgggcgcg ccgggtggtg
      181 gacgagctgc tgcgcttcgg cgagctgcgc cccgtgtgga cgggggcccg gttggtgacc
      241 gtcgacccgg agatggcgcg gcgcgccggc ctgccggtgc cgaggggcgc ggcggtgctc
      301 aaggtctatc cggactcgcc ggcgacggcg gccggcctgc gcgagcagga cgtcatcgtc
      361 ggcgtcaacg gtcgccccgc ggcgagccgc gaggaggtgg ccacggcgct ctacacggtg
      421 actcccggca cgccggtgcg tctcgacgtc cgccgcggcg agcagacgct gcagctcacc
      481 ctgcgggcgg tgcagccgcc ggagcgtctc gggatggagc tcctgcgcgt gggggtcggc
      541 ctggtgaccg aggagagccg caacggcttg gtggtccagc gggtgatcgc cggctccgag
      601 gcggccgaac gggggctgcg gccgggggac gtggtgctcg gggccaacgg ccagcgggcg
      661 cgcacccacg acgagctgag ccgcgaggtg ctgcgcggca tcgagcgcgg cgggctgctc
      721 ctcgccgtcc agcgcggcag gtacgtctac aacatcggct tcccgctgta gggaggtgag
      781 ggaaagcagg gacacagggc gttctcctct gcgtgacgcg aagtgaatcg caaccagagg
      841 agaacgatcc gatgtcccag tccaaactcg cggctccgca gttggtgatc aatagccgca
      901 tgatttattc gagctggatc ccggcggacc ccaaggccgc cgcggcgctg cttccggacc
      961 gcctgacccc ggcggccaac ggtgccatct acatgaatca gtacgtcgtc gattcggagg
     1021 agcagacctc cggcttcggc gcctactcgc tgacctacat gggcctcgac ctgggcaacg
     1081 tgacggcgcc ggacggcgtc accccgggcc ggtttttcac caactatttc aactcgtccg
     1141 agatcatgcg cgagtacgtc cgcgagcgcg gcgtgccggc gacgccgggc acgaccacgg
     1201 tcgaggtgca cggccacgag ctggaggcga cgaccttcgt ggacggtgtc gcgatcatcc
     1261 gcacccgggc gcgggtgggc aagaaggtcg gcgtcgtggc gcggggacat ctcacctatg
     1321 tcacgcagct cgacggtaag ctcatcgccg gcaactacgc ctacatcggc gagctggtcg
     1381 atcccttcga gctgcagtcg gtggagttcc tcaccaagga ccatccggcc tgggcgttgc
     1441 ggccggccca gccgctgcag gtggtgaccg gctcgtcctg gttcgccccg cgcgactcct
     1501 tcgtctaccc cggcggcgag cgcgaaatct gatccattcc tcaggaaggg agccccaccg
     1561 gcgccctgcc ggaggtctcc agcaggcgct cgaccccctc ggggtcgagc gcctggcaga
     1621 gcaggaagcc ctggccgtag tcgcagtgca tcgccttgag acgggagagc tgcccctccg
     1681 tctccacccc ctccgccacc accttcatcc gcaggctgtg cgccagaccc acgacggcgc
     1741 ggacgatctc gtccttctcg ccgtcaggcc ccatgccgcg gatgaacgac cgatcgattt
     1801 tcaggatgtt caccgggaac tgctggagcg agctcaacga cgagtagccg gtcccgaagt
     1861 cgtcgacgca caggccgatc tccagcgcct tcagatgccg cagcaggtcc gcggcggcct
     1921 ccgggttctc gaggatcgag ctttcggtga tctccagccg cacgctcggc gccggcaggc
     1981 ccgtgcggtg gaggatcagg cggatctgct cgatgagatc ggtctgcagg agctggcgcc
     2041 gcgacaggtt gacgttgatc gccagcggcg gatcggacgg atactcctgc tgccaccggt
     2101 gtagcgcctc gcaggcttcg gtgagcaccc agcggccgat ggggaggatc agcccggtct
     2161 cctccgcgac gtggatgaac tgatcggggg ggacgaggcc ccaggccgga tgccaccagc
     2221 gca
//
LOCUS       DASPJI010000705         3392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_156088, whole genome shotgun sequence.
ACCESSION   DASPJI010000705 DASPJI010000000
VERSION     DASPJI010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156088"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3292)
                     /locus_tag="VEL74_19835"
     CDS             complement(<1..3292)
                     /locus_tag="VEL74_19835"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64842.1"
                     /translation="MDRISRSFRPAPVGVAGELLIGGVGLAQGYLGRPELTAERFVPD
                     PFAALWSEPGARVYRTGDLARHLAGGEIEFLGRIDFQVKIRGFRIELGEIEAALLAHP
                     GVREAVVLVREDRPGDPYLVAYVVGSFLGAEELRAYLQERLPEAMVPPAFVMLDALPL
                     NPNGKLDRRALEKIVPSREERAGIDEYAAPRTPSEEILAGIFAEVLGQERVGIHDGFF
                     ELGGHSLLATQVVSRVRQAFGVELAIRELFEAPMVARLAERIERIGTSTIEAPPIVPV
                     PRGRDLPPSSAQQRLWFVDRLKPGSSAYNVPLAVRLTGTLDAAALGHALRGMARRHEV
                     LRTTFAERDGHPVQVIAPVPRLSLEIVDLSGLPEAEREDELTWQATTEALRPFDLAAG
                     PLVRATLLRLGEEEHALLVTMHHIVTDGWSMGLFLRELTALYGGRELPELPVQYADFA
                     VWQRSWLSGEVLESQLSYWRGHLAGAPARLTLPTDRPRPAVTTNHGATRPVVLPSSLS
                     QELSALARRQGATPYMVLLAGFAALLGRYSGQRDVVVGSPIAGRNRREIEGLIGLFVN
                     ALALRVELADDLGFAGLLRQVRRTALEGFSHQDIPFERLVEELVSERDLGHSPLFQVT
                     LAVQNVPSLSDGDTAISGLAIRDVAVESRTAKFEMSLRLVPRGDELAGGLEYNTDLFD
                     GATAERLIGHLIRLLEGAVTTPEIPVPDLALLAPAEQQHLLREWNDTGTDYGAPSGAC
                     LHELIQAQVARTPDAVAAVSGDEHLSYRGLARQAEGLAWRLVAVGVGPDSVVGICAER
                     SLEMIVGLLGVLEAGGAYLPLDPSYPRERLATILESAEATAVLVQEHLEDLLPASPIP
                     RLLLGPGCEDIAWTPPGTRPENLAYVIFTSGSTGKPKGAMVSHRAIVNRLLWMQQAYG
                     LSPADRVLQKTPFGFDVSVWEFFWPLLVGAPLVFARPEGHKDPAYLAELIAEQRITVL
                     HFVPSMLRALLETARWASCVSVRAVMASGEALPFDLTERFFDGMPGELHNLYGPTEAA
                     VDVSFWACVPGDERRLVPIGRPIANLRLHVAERNWQPAPLGVAGELLIGGVGLGRGYL
                     GRPDLTAER"
BASE COUNT          551 a         1317 c         1094 g          430 t
ORIGIN      
        1 accgctccgc cgtcaggtcc ggccggccca ggtagccccg cccgaggccc acgccaccga
       61 tcagcagctc tccggccacc cccagaggcg ccggctgcca attccgctcc gccacatgca
      121 ggcgcaggtt ggcgatgggg cggccgatgg gaaccagccg ccgctcgtcg cccggcacac
      181 aggcccagaa gctgacgtcc accgccgcct cggtcgggcc gtagaggttg tgcagctcgc
      241 ccggcatccc atcgaagaac cgctcggtca gatcgaacgg cagcgcctcg ccgctggcca
      301 tcacggcccg taccgagacg cagctcgccc accgggccgt ctccaggagg gcccgcagca
      361 tcgaggggac gaagtgcagc accgtaatcc gctgctccgc gatcagctcg gccagatagg
      421 cggggtcctt gtgcccctcg gggcgggcga acaccagcgg cgctcccacc agcagcggcc
      481 agaagaattc ccacaccgac acgtcgaagc cgaatggcgt tttctggagc acccggtcgg
      541 ccgggctcag gccataggct tgctgcatcc agagcaggcg gttgacgatc gcccggtgcg
      601 acaccatcgc ccccttgggt ttgccggtcg agcccgaggt gaagatgacg taggccaggt
      661 tctcgggccg ggttccggga ggcgtccagg caatgtcctc gcagcccgga cccagcagca
      721 gccgcgggat gggcgacgcg ggcaggagat cctccagatg ttcctggacg agcaccgccg
      781 tggcctcggc gctctccagg attgtcgcca gccgctcccg cggatacgag gggtccagcg
      841 gcagataggc gccgccggcc tccagcaccc cgagcaggcc cacgatcatc tccagcgacc
      901 gctcggcgca gatccccacc accgagtccg gcccgacccc caccgccacc agccgccagg
      961 ccagaccctc cgcctgccgg gcgagtccgc ggtagctcag gtgctcgtct ccagagaccg
     1021 ccgccactgc gtcgggcgta cgcgccacct gggcctggat cagctcgtgc aggcaggcac
     1081 cggacggcgc tccgtagtcc gttccggtgt cgttccactc ccgcagcaga tgctgttgct
     1141 cggccggcgc cagcagggcg agatccggca ccggaatctc cggtgtcgtg acggcgcctt
     1201 ccagcagtcg gatcaggtgc ccgatcagac gctccgcagt ggccccgtcg aacaggtcgg
     1261 tgttgtactc caggccgccg gccagctcgt ccccccttgg caccagccgc aggctcatct
     1321 cgaacttcgc cgtccgggac tccaccgcca cgtcccgaat tgccagcccg gagatcgccg
     1381 tatctccatc cgacagcgac ggcacgttct gcacggccag agtcacctgg aacagcggtg
     1441 agtgccccag atcgcgctcg ctcaccagct cctcgaccag acgctcgaac gggatgtcct
     1501 ggtgggagaa gccctccagc gccgtccgtc gcacctgccg cagcaggccg gcgaagccca
     1561 ggtcatccgc cagctccacc cgcagcgcca gagcgttgac gaagagcccg atcagcccct
     1621 cgatctcccg ccggttgcgc cccgcgatgg gcgagcccac caccacgtcc cgctgcccgg
     1681 agtaacggcc cagcagagcc gcgaaacccg ccagcagaac catgtacgga gtagccccct
     1741 ggcgccgggc cagggccgac agctcctgcg aaagggacga gggcaacacc accggccggg
     1801 tagccccgtg gttggtcgtc accgccggtc gcggccggtc ggtgggcagg gtcagccggg
     1861 ccggcgcccc ggccaggtgc ccccgccagt aggacagttg cgactccaac acctcgcccg
     1921 acagccaaga ccgctgccag accgcgaagt ccgcgtactg caccggcagc tccggcaact
     1981 cgcggccacc atagagcgcc gtcagctcgc gcaggaagag gcccatggac cagccgtccg
     2041 tgacgatgtg gtgcatggtc accagcaggg cgtgctcctc ctcgcccagc cgcagcaacg
     2101 tcgcccgcac cagcggcccg gccgccaggt cgaacggccg cagcgcctcg gtcgtcgcct
     2161 gccacgtcag ctcgtcctcg cgctccgcct ccggcaggcc cgagaggtcc acgatttcga
     2221 gcgacaggcg cggcacgggc gcgatcacct gcacgggatg gccgtcccgc tcggcgaacg
     2281 tggtgcgcag cacctcgtgg cggcgcgcca tcccgcgcag cgcgtggccg agcgcggcgg
     2341 catccagcgt gccggtgagc cggacggcca gcggcacgtt gtaggcggag ctgcccggct
     2401 tgagccggtc cacgaaccag agacgctgct gggcggacga cggcggcagg tctcgacccc
     2461 gcggcaccgg tacgatcggc ggcgcctcta tggtggaggt cccgatccgc tcgatcctct
     2521 cggccaaccg ggccaccatc ggtgcctcga acagctcgcg aatcgccaac tccacgccga
     2581 aggcctggcg tacccgcgac accacctggg tggccagcag cgagtgcccg ccgagctcga
     2641 agaagccgtc gtggatcccg acccgctcct gccccagcac ctcggcgaag atccccgcca
     2701 ggatctcctc ggatggcgtt cgcggcgcgg cgtactcgtc gatcccggcg cgctcctccc
     2761 tggagggaac gatcttctcc agcgccctgc ggtccagctt gccgttggga ttgagcggca
     2821 gggcgtccag catgacgaag gccggcggca ccatggcctc gggcagacgc tcctggaggt
     2881 aagcgcggag ctcctcggca cccaggaagg agcccaccac ataggccacc aggtacggat
     2941 cgccagggcg gtcttcccgc accagcacca ccgcctcccg cactccggga tgagccagca
     3001 gcgccgcctc gatctccccc agctcgatac ggaagccccg gatcttgacc tgaaagtcga
     3061 tccgccccag gaactcgatc tcaccaccgg ccaggtgccg cgccaggtcc ccagtccggt
     3121 agacccgcgc ccccggctcg ctccaaaggg cggcgaacgg gtccggcacg aagcgctcgg
     3181 ccgtcagctc cggacggcca aggtacccct gcgccagacc cacgccgccg atcagcagct
     3241 ccccggccac tcccaccggc gccggacgga acgatcggga gatgcggtcc accacatgca
     3301 gccgcaggtt ggagatcggc cgcccgatcg gcaccgacgt accgacggcg ttcccggccg
     3361 tccaggcgct cacctcgatg gaggcctcgg tg
//
LOCUS       DASPJI010000706         6981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_45376, whole genome shotgun sequence.
ACCESSION   DASPJI010000706 DASPJI010000000
VERSION     DASPJI010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45376"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VEL74_19840"
     CDS             <1..489
                     /locus_tag="VEL74_19840"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HYG64843.1"
                     /translation="GGNVFTAVRGGGARWNGRRMAVSSRPGLEGAFLATGYPFRALPT
                     LDVYLEIFRDVFRQAKAIRRCGSAALDLAYTAAGVYDGFFEFRLSPWDIGAGVLMIEE
                     AGGRVTDLDGGSRYFAGGNIVSGGGAVQRELLEVARRHASEAVIDRLNPIDVPAEDPV
                     PR"
     gene            500..1663
                     /locus_tag="VEL74_19845"
     CDS             500..1663
                     /locus_tag="VEL74_19845"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYG64844.1"
                     /translation="MNLGRLGRLVRWPARGAAAATRSGAPRTGGEGWSLEVQVHPSDI
                     RKRVRYFFLSRVQVTFWSILALLYLMALAVAAGVAPMVIKGYLGKDDYQTLVAERARQ
                     GERLQELVGRLGEIEARSQGLAARVDKIFLVYGLPAVPVPPAAANAANAANAANAGAT
                     DGADPVAAAALYAGTLEQGERRHRRISRRLRSLDLSLERAREFERAHPEQVRGTPALC
                     PLRGELVLISPFGRRRNPFTRELELHPGVDLAAPLGSPVHAPASGVVVYAGRYPLGRS
                     AIWWRLGNLVAVRNGDDLVTLYGHLQEMRVRPGQRVERGDLLGTVGRSEVNNSVHLHY
                     EVRRRAGAGELRPADPLTYILDRRWPNEERLLARARSGPPVTGFEPLPREIAR"
     gene            1660..2451
                     /locus_tag="VEL74_19850"
     CDS             1660..2451
                     /locus_tag="VEL74_19850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64845.1"
                     /translation="MRQDLSSPTLIIPRGTRIEVDPEGQLSIQAPGNLVIQNSGKYGT
                     LESVGGSIRIDRGVEVEANTVRCTETCYVQGFLTAWKVIARSLQLEESARAHVVLQET
                     ERLEIGRDARLVGNFSSEKEMVSLFSRFAHQVRSIPFLLDRKAPRPAELSDGLDETID
                     IVPQAEAEPAPEMSAPVPVPPLAELPDPLLFALVLLQRESERRIHPPAGQRAIDELIK
                     LLEQRDVETLAFTYRTLFNRVPEPRDDARRARQMVTEHFAAHPPA"
     gene            2665..3729
                     /locus_tag="VEL74_19855"
     CDS             2665..3729
                     /locus_tag="VEL74_19855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64846.1"
                     /translation="MRRTSLWLLPLAFLALPLLAQPQSPPLSCGAKFLGPLSAEGLLD
                     AGTYQPLAAEQRLYYGLTGLGDEALEIRYLVADELYLTENLDLSTSRLPRAEIPGRKP
                     LRQAESTLDFQELLEGEWMVELLALRPDLVRRLHELAQQGSRIEVQVFQAGRRLAALP
                     FKGILRRSTELGASGAVQVVVHSTVRGIGERAEPRNPPLLGTKVLASCSECLDEMPCD
                     TECGYDPGKGGPVTCGENGAPCGGSTCECSRVLYDSWTSWYFNRAYAYSPATYDCLKS
                     GSSFYTTHQVYILEYRRDLVRSSMICPNCPSCVGCYFHQQVIDYQLTTSYCYYNVGGG
                     CGFGRTAACYELCSMTSYCN"
     gene            complement(4251..4385)
                     /locus_tag="VEL74_19860"
     CDS             complement(4251..4385)
                     /locus_tag="VEL74_19860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64847.1"
                     /translation="MGALNGVIHLLQAPESLAYVMEAAGGVALERCGAILDERVATPK
                     "
     gene            complement(4373..4801)
                     /locus_tag="VEL74_19865"
     CDS             complement(4373..4801)
                     /locus_tag="VEL74_19865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64848.1"
                     /translation="MRSSEYDSTPSVHPNAFKTAFLGRLAERDEPPTAGEADVAGPWR
                     IEPIPGHGFGLFRVGESLGRGFAPTAVFPSRWLALLLAGILPGTGKDLPLILSKQPDA
                     HGLYPIALAGGGDDGDGGDGGDNGHRSASFSSSTRRRWAR"
     gene            5187..6278
                     /locus_tag="VEL74_19870"
     CDS             5187..6278
                     /locus_tag="VEL74_19870"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64849.1"
                     /translation="MASDAIVKEVSTFTPTAVEQQVFTLSCLSGMASKSQMSQSDMQE
                     QLRVWEANIGTWKAVWGPCYTFKPLATIPCNTMYVAQALDAAQPTYVVAIAGTVAGSF
                     YDWMTEDLNIAPVDWPHASNAGQVTTGDNDGLNNLLKMTYEGQTLQEFLSSITNKSSA
                     SLTFTGHSLGGALAPMLTLALMDPASTLKAENDVSIGNWAEVSLLATAGPAIGDVTFV
                     QYFNAALAAASCTFIWNVNDVVPHAWNQATMMELTSPTNIYNLTLDPSQCLANKLGYY
                     QGEAGLYTYTQFEQVPAFAGVLQPYTMSISQGTWTPESEFLAQALYQHINAYGIAFNC
                     TGWVKLIADPCADPKLAAELLAAVNALCE"
     gene            6433..6639
                     /locus_tag="VEL74_19875"
     CDS             6433..6639
                     /locus_tag="VEL74_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64850.1"
                     /translation="MSYDNIPDVRDGLTRQERIVLTVLYQVQKERGDRNVPTAMLWGR
                     VCEHFYISPEDLSEMLARLGARKW"
     gene            complement(6647..>6981)
                     /locus_tag="VEL74_19880"
     CDS             complement(6647..>6981)
                     /locus_tag="VEL74_19880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64851.1"
                     /translation="RRFLQSLAKVVAVPWLLTSSEDLRYPQVDGRRPFWLKGLQAYTR
                     RVFQLCGHDAAAYDRLIRVLHLLDGPEQLFHPEVLAGVLGTLNPFSLKLPPLPERGQG
                     EAGEGARG"
BASE COUNT         1153 a         2312 c         2315 g         1201 t
ORIGIN      
        1 gggggcaacg tcttcaccgc ggtgcgcggc ggcggcgccc ggtggaacgg ccggcggatg
       61 gcggtgtcgt cgcggccggg gttggagggt gccttcctcg ccaccggcta ccccttccgc
      121 gccctgccga ccctggacgt ctatctggag atcttccgcg acgtgttccg gcaggccaag
      181 gcgatccgcc gctgcggctc ggccgccctg gacctggcct acaccgccgc gggggtctac
      241 gacggcttct tcgagttccg gctctccccc tgggacatcg gggccggcgt cctgatgatc
      301 gaggaggcgg gggggcgggt cacggacctg gacggcggga gccggtactt cgcggggggc
      361 aacatcgtgt cggggggcgg cgccgtgcag cgggagctgc tggaggtggc gcggcgccac
      421 gccagcgaag cggtgatcga tcgactgaac cccatcgacg tgccggccga ggacccggtg
      481 ccacggtgac ggcccgcgca tgaatctcgg acggctcggt cgtctcgtcc ggtggcccgc
      541 gcggggcgct gccgccgcca ctcgcagcgg cgccccccgc acgggcgggg agggctggag
      601 cctggaggtc caggtccacc cgagcgacat ccgcaagcgg gtccgctact tcttcctctc
      661 ccgcgtccag gtcaccttct ggtcgatcct ggcgctcctc tatctgatgg ctctcgccgt
      721 ggccgccgga gtggcgccga tggtgatcaa gggatatctg ggcaaggacg attaccagac
      781 cctggtcgcc gagcgcgccc ggcaggggga gcgcctgcag gagctcgtcg gacgcctcgg
      841 cgagatcgag gcgcgctccc aggggctcgc cgcccgggtg gacaagatct tcctggtgta
      901 cggcctgccc gccgttcctg ttccccctgc cgccgccaat gccgccaatg ccgccaacgc
      961 cgccaatgcc ggggcaaccg acggcgccga tccggtcgcg gcggccgcgc tgtacgccgg
     1021 gaccctcgag cagggggagc gccggcaccg gcggatctcc cgccggctgc gaagcctcga
     1081 cctctccctc gagcgggcgc gggagttcga acgcgcccat ccggagcagg tgcgcggcac
     1141 gccggccctc tgcccgctgc gcggcgagct cgtgctgatc tcccccttcg gccggcggcg
     1201 caatcccttc acccgggagc tggagctcca ccccggcgtc gatctggcgg cgccgctggg
     1261 cagcccggtc cacgccccgg ccagcggcgt cgtggtctat gccggccgct atcccctcgg
     1321 ccgcagcgcc atctggtggc ggctcgggaa cctggtggcg gtgcgcaacg gcgacgacct
     1381 ggtcaccctc tacgggcatc tgcaggagat gcgggtccgg ccggggcagc gggtggagcg
     1441 gggggacctg ctggggaccg taggccggtc cgaggtgaac aacagcgtgc acctgcacta
     1501 cgaggtccgg cgcagagcag gagccggcga gctgcggccg gcggacccgt tgacttacat
     1561 cctggaccgc cgctggccca acgaggagcg gctcctggcg cgggcccgca gcggtccgcc
     1621 cgtcaccggc ttcgagccgc tgcccaggga gatcgcgcga tgaggcagga cctgagctct
     1681 cccaccctga tcatcccccg gggcacccgt atcgaggtgg acccggaggg gcagctctcc
     1741 atccaggcgc cgggcaacct ggtgatccag aactcgggca agtacggcac cctggagtcg
     1801 gtgggcggct cgatccgcat cgaccgcggc gtcgaggtgg aggccaacac ggtgcgctgc
     1861 accgagacct gctacgtgca ggggttcctc accgcctgga aggtgatcgc ccgctccctg
     1921 caactggagg agtcggcccg cgcccacgtg gtgctccagg agacggagcg cctggagatc
     1981 ggccgcgacg cccggctggt gggcaacttc tcttcggaga aggagatggt gagcctcttc
     2041 tcccgcttcg cccaccaggt gcggtcgatc ccgttcctgc tcgaccgcaa ggcgccgcgc
     2101 cccgccgagc tgtccgacgg cctggacgaa acgatcgaca tcgtgccgca ggccgaggcc
     2161 gagccggctc ccgaaatgtc ggcgccggtg ccggtgccgc ccctggccga gctgcccgat
     2221 ccgctcctct tcgccctggt gctcctgcag cgggagtcgg agcggcgcat ccacccgccg
     2281 gcggggcagc gggcgatcga cgagctgatc aagctgctgg agcagcggga cgtcgagacc
     2341 ctcgccttca cctaccggac gctcttcaac cgcgtgcccg agccgcggga cgacgcccgc
     2401 cgcgcgcgcc agatggtgac cgagcacttc gccgcccacc cgccggcctg agagggccca
     2461 ggaaaactac cttgtcgatg ggcgaagaag atctgcgccg ctttgggtaa gagcccggtg
     2521 gcttcgcgct ttacaaacct ctttgctcgt gcgcataata cttgtaaatg cttcacgatg
     2581 tggcgggggt cggcaccacc gcagctgagg aggctacgag cccgccgacg gacctcccgc
     2641 tcacacgaga acaggaggag gaaagtgcga cgcaccagtt tgtggcttct gcccctcgct
     2701 tttctggccc ttcccctatt ggcccagccc cagtcgccac cgctctcctg cggtgcgaag
     2761 ttcctcggcc cgctgtcggc cgaggggctg ctggacgcag gcacctacca gcccttggcc
     2821 gcggagcagc gtctctacta tgggctcacc ggcctgggtg atgaggcctt ggagatccgg
     2881 tacctcgtcg cggacgagct gtatctgacg gagaacctgg acctttccac ctcccggttg
     2941 ccgcgggccg aaattccggg ccgcaagccg ctccggcagg ccgagagcac cctcgatttc
     3001 caggagcttc tcgagggaga gtggatggtg gagctcttgg ccctgcgccc cgatctcgtc
     3061 cggcggctcc acgagctcgc ccagcaaggc tcccgcatcg aggtccaggt cttccaggcc
     3121 ggccggcggc tcgcggcgct gccgttcaag gggatcctgc ggcgcagcac cgagctgggt
     3181 gccagcggcg cggtgcaggt ggtggtccac tcgacggtcc gtggtatcgg tgagcgcgcg
     3241 gagccgcgaa atcccccgct cctcggtacc aaagtcctcg ccagttgctc ggagtgtttg
     3301 gacgagatgc cgtgcgacac cgagtgcggc tacgatccgg gcaagggcgg cccggtcacc
     3361 tgcggcgaga acggggcgcc gtgcggcggc tccacgtgtg aatgctcgcg cgtgctctat
     3421 gacagttgga cctcgtggta cttcaaccgt gcgtacgcct acagcccggc cacctacgac
     3481 tgcctgaaga gcggctcgtc tttctacacc actcaccagg tatacatctt ggagtaccgc
     3541 cgggatctgg tccggagcag catgatctgc cccaactgcc cgtcctgtgt cggctgctac
     3601 ttccatcagc aggtgatcga ctatcagctc accacctcgt attgctatta caacgtcggc
     3661 ggcggttgtg ggtttggacg cacggctgcg tgctacgagc tctgctcgat gacctcctac
     3721 tgcaactgaa cccttgccag tcttccccct ctctccctcg cttcagggag agagggggct
     3781 ttccgagcca tagcgcaacg gcaaatgcag tcgtgccacg gcgtttgccg gcgcctcccg
     3841 gcgtttgaag tcgcctcgct gaaaacgaag ccggctcccg gcgttcaaag ttatcccgcc
     3901 gcgtttgagg cggcccacgg cctttgaagc tgcctcattg aaaacgaagt cgtctcccgg
     3961 cgtttgaagt tgccccgtcc catctgaagt cgtcccgccg cgtttgaaat cgtcccgccg
     4021 cgtttgaagt cggctcacgg cgtttgccgg cggcccacgg cgtttgaagt cgtctcattg
     4081 gaaacgaagt cggctcaccg caaatgaagt cgagccgact tcatttgccg tggcgcgccg
     4141 gatctgacgc gcgagctcct ggacagcggg aaggggtctt ctacggagcg gagaggagga
     4201 cgcggccgac gagtgggctt gccggccgcg ccctctataa agaggatgtc ctacttcggc
     4261 gtggccaccc gctcatcgag gatggcgccg caccgctcga gggcgacccc gcccgccgcc
     4321 tccatcacgt aggccagaga ctccggagcc tggaggaggt ggatgacgcc gttcagcgcg
     4381 cccaccgcct tctcgtcgaa gagctgaagg aagccgaccg gtggccattg tccccgccat
     4441 cgcctccgtc gccatcgtca cccccgccgg cgagggcgat ggggtagagg ccgtgagcgt
     4501 ccggctgctt ggagaggatg agaggcaggt ccttgccggt gccggggaga atgcccgcca
     4561 ggaggagcgc cagccagcgg gaggggaaga ccgccgtcgg ggcgaagccg cgacccaggc
     4621 tctcgcccac ccggaatagg ccgaagccat gccccgggat gggttcgatg cgccagggcc
     4681 cggccacatc ggcctcgccg gccgtgggcg gctcgtcccg ctcggccaga cggccaagga
     4741 acgcggtttt gaacgcgttg ggatgaacgg acggagtgct atcgtactcg gagcttcgca
     4801 tagatcctcc taacaaggat gtttgcgggg tcaggcctgg ggggagagtt ggtagctctc
     4861 ttccgggccg taagcaggac accacgccct gccgtccccc cttccagaga tgttgtcggg
     4921 gactgtctga accttcttcc tcttcgcctc gatcccccac ctcgttggag aggctacccc
     4981 agcaaggaag aggcgcgagc cgattccacg atatgcatgt agagcttgac aaaacagaga
     5041 gacccatttt agccttcccg acatctttgc tcgacgaggt acggggggac agagccggac
     5101 aagaagaatt tccggtcgtg atggggcctt gtgtcttcgt gcgatgcagt tgatcatcat
     5161 tcactaccgg tatgggaggt ctcatcatgg ctagcgatgc gattgtcaaa gaagtctcca
     5221 cgttcacgcc tacggcagta gagcaacagg tgttcacgct ttcgtgtctc tcgggcatgg
     5281 ccagcaagag ccagatgtcc cagagcgata tgcaagagca actccgagtt tgggaggcga
     5341 acatcggcac gtggaaagcg gtatggggac cgtgttacac cttcaagccc ttggcaacga
     5401 ttccgtgcaa cacaatgtac gtggcgcagg ctctcgatgc ggcccagccg acctacgttg
     5461 tcgcaatcgc cggtacggtc gccgggtcgt tctacgactg gatgactgag gatctgaaca
     5521 ttgcccctgt ggactggccg cacgcctcga acgcgggtca ggtcaccacc ggcgacaatg
     5581 acggcctgaa taatctgctt aagatgacct acgaggggca gacgctccaa gagttcctct
     5641 ccagcattac caacaagtcc agtgcatcgc tcacgttcac cggacacagc cttgggggtg
     5701 cgcttgcgcc aatgctgact ttggccctca tggatcccgc ttcgaccttg aaagcggaga
     5761 acgacgtgag cattggcaac tgggctgagg tctcgctcct ggccaccgcc gggccagcga
     5821 tcggggatgt gaccttcgta cagtacttca acgccgcgct cgccgccgct tcgtgtacgt
     5881 tcatctggaa cgtcaacgat gtcgtccctc acgcctggaa ccaggcaaca atgatggaac
     5941 tgacctcgcc gaccaacatt tacaacctga cgctcgatcc gagccaatgt ttggcgaaca
     6001 aactggggta ctaccagggc gaagccgggc tctatactta tacgcagttc gagcaggtcc
     6061 cggcctttgc cggggtgcta cagccctata ccatgtcaat cagtcagggt acgtggactc
     6121 ccgagagcga gttcctggcg caggctttat accagcatat caatgcgtac ggaattgcat
     6181 ttaattgcac agggtgggtt aagctgatcg cagatccttg cgccgatccg aaattagcgg
     6241 cggagttact ggcagcggtg aacgcgctct gcgagtgact tcactcggcg cctcgtcgcg
     6301 cctccaaggt acctgcaccg tctagctggg gtatttgctc caagttactg atttagctag
     6361 gatgtgcccg gtaggcgagt cctatcaaaa ggcaggcatt atccaggtgg tattgggcgc
     6421 ggtacgtaaa tcatgagcta cgacaacatt cccgacgtcc gtgatggtct gacgcgccag
     6481 gagcgcatcg tcctgacggt gctgtaccag gtgcagaagg agcgcggcga tcgcaacgtg
     6541 cccaccgcga tgctctgggg ccgtgtgtgc gagcactttt acatcagtcc ggaggatctc
     6601 tccgagatgc ttgcgcggct cggcgcgcgc aagtggtgat cagccctcac cccctggccc
     6661 cctctcccgc ctccccttgc cccctctccg ggagaggagg gagcttcaac gagaacggat
     6721 tgagggtgcc gagcacgccc gccaacacct ctggatggaa gagctgctcc ggcccgtcca
     6781 ggaggtggag cacccggatc agacggtcgt aggccgcggc gtcgtggccg cagagctgga
     6841 agacgcggcg cgtgtaggcc tggagccctt tgagccagaa gggacgccgg ccatcgacct
     6901 gggggtagcg tagatcctcg gacgaggtca gcagccaggg aacggctacg accttggcca
     6961 gcgactggag gaagcggcga g
//
LOCUS       DASPJI010000707         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_612908, whole genome shotgun sequence.
ACCESSION   DASPJI010000707 DASPJI010000000
VERSION     DASPJI010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612908"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="VEL74_19885"
     CDS             <1..1238
                     /locus_tag="VEL74_19885"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HYG64852.1"
                     /translation="TVANEDPVLSRGTFVVLATIALTVSGVVIAIGTSAPLLTSFMEN
                     PGQVGPEFYNRVNLPIALLMAGLLALVPYLTWKGTEPRELAGKLLWPGLFSLLVTGGA
                     VAAGVRDPFHVVFVLLAALALASNFQKVLARARAGGFSLRGAGGYFAHVGVAIMLLGF
                     MASSAYDQSAKVTLEQGKPEKIEDMTLTFTRFIPRQGEEKEKMEVEVVKDDGERFFVY
                     PRFFMNERTRQTMVHPDIRKTALADFYVSPIEFDPGQPRLELAAGESRQVGDMEVRFA
                     GFDLNAGGTNAMAQMESGGPMTIGAKVEVTQAGRTTQITPIYQMNPATGQVETPPLPL
                     PGGGAVYVSGLNATNGAVQLEVMGVANPARLSVDVTRKPLINLVWYGLYVVLAGGAMA
                     ALQRLREARIRDQVAAAGE"
     gene            1327..1530
                     /locus_tag="VEL74_19890"
     CDS             1327..1530
                     /locus_tag="VEL74_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020563963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HYG64853.1"
                     /translation="MRLKIVVHEAEEGGYWAEVPAIPGCATQGETLDELMTNVREAVE
                     ACLSVDLKEIDLTDTDQVLEIAV"
     gene            1527..1733
                     /locus_tag="VEL74_19895"
     CDS             1527..1733
                     /locus_tag="VEL74_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002737289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HYG64854.1"
                     /translation="MKSVSGKELARILERNGWRLQRVSGSHHVYAKEGNPARISLPIH
                     GNHPLKLGLQRHFMKVAGLQDSDL"
     gene            1857..>2242
                     /locus_tag="VEL74_19900"
     CDS             1857..>2242
                     /locus_tag="VEL74_19900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64855.1"
                     /translation="MWWLGYPFLVGGVLVLNILFRNRRFLNLWQDAVTACKLTRQEMT
                     SIWAPRVNLTARTETLQVSFDAGHKGKTWLQVRVPDLPGFADMKLRRTMYALFGGAEI
                     QAGDEPFDQEFFIEGPVQLGTAMLDA"
BASE COUNT          381 a          728 c          770 g          363 t
ORIGIN      
        1 ccacggtagc caacgaggac ccggtgctct cccgcggcac cttcgtagtg ctggcgacga
       61 tcgcgctgac ggtctccggg gtggtgatcg ccatcggcac ctcggcgccg ctcctcacca
      121 gcttcatgga gaaccccggt caggtcggcc ccgagttcta caaccgggtc aacctcccca
      181 tcgcgctgct gatggcgggg ctgctggcgc tggtccccta cctcacctgg aaagggacgg
      241 agccgcggga gctggccggg aagctgctct ggcccggcct gttcagcctg ctggtgaccg
      301 gcggcgcggt ggcggccggc gtgcgcgacc ccttccacgt ggtcttcgtc ctgctcgccg
      361 cgctggcgct ggcgagcaac ttccagaagg tgctcgcccg ggcgcgggcc ggcggtttca
      421 gcctgcgcgg cgccggcggc tacttcgccc acgtcggcgt ggccatcatg ctgctcggct
      481 tcatggcctc ctcggcctac gaccagagcg ccaaggtgac gctggagcag ggcaagcccg
      541 agaagatcga ggacatgacc ctcaccttca cccgcttcat cccccgccag ggggaggaga
      601 aggagaagat ggaggtcgag gtggtcaagg acgacggcga gcggttcttc gtctatcccc
      661 ggttcttcat gaacgagcgg acgcgccaga cgatggtcca cccggacatc cgcaagaccg
      721 cgctggcgga cttctatgtc tcgccgatcg agttcgatcc cggccagccg cgcctcgagc
      781 tggccgcggg ggagagccgc caggtggggg acatggaggt caggttcgcc ggcttcgacc
      841 tgaacgccgg cggcaccaac gcgatggccc agatggagag cggcggcccg atgaccatcg
      901 gcgccaaggt ggaggtgacg caggccggcc gcaccacgca gatcacgccg atctatcaga
      961 tgaacccggc gaccggccag gtggagaccc cgccgttgcc gttgccgggc ggcggcgcgg
     1021 tctacgtgtc gggcctgaac gccaccaacg gcgcggtcca gctcgaggtg atgggcgtcg
     1081 ccaaccccgc ccgcctgtcg gtggacgtca cccgcaagcc gctgatcaac ctggtctggt
     1141 acggcctcta cgtcgtgctg gccggcggcg ccatggcggc cctccagcgc ctgcgcgagg
     1201 cgcgcatccg cgaccaggtg gcggcggccg gcgaataagc ggccacgata gaatgccagg
     1261 cgccgccccc ttggcaggga gcggcgcctt ttcttttcac gggactgctg gatcaaggaa
     1321 ccacgcatgc gactgaagat cgtcgtccac gaggcagaag aaggcggcta ctgggccgag
     1381 gtacctgcca tcccgggctg tgccactcaa ggggagaccc tcgacgagct catgaccaat
     1441 gtacgggagg cggtcgaggc gtgcctgtcg gtggatctca aggagatcga tctcaccgat
     1501 acggatcagg tcctggagat cgccgtgtga agagcgtctc cgggaaggag ctcgctcgca
     1561 tattggagag gaacggctgg agactccaga gggtcagcgg cagccatcac gtctacgcca
     1621 aagaggggaa tccggcgaga atctccctac ctatccacgg aaaccacccc ttgaagcttg
     1681 ggcttcagcg ccacttcatg aaggtggcgg ggctccagga ttcagatctc tagggaccag
     1741 gagacagagg cgccgccccc ttggcaggga gcggcgcctt ttcttttcag ccggaacgcg
     1801 ggcgccgcga gggatcgggc ggagcgcgcc tataatcagg tcgcgaggtt cccgatatgt
     1861 ggtggctggg ctatcccttt ctggttggcg gcgtccttgt cctgaacatc ctgttccgca
     1921 accgccggtt tctgaatcta tggcaggacg ccgtcacggc ctgcaagctg acccggcagg
     1981 agatgacttc catctgggcg ccgcgcgtga acctcacggc gcggacggaa acgttgcagg
     2041 tcagcttcga cgcgggccac aaaggaaaaa cctggctcca ggtgagggtt ccggatctgc
     2101 ccggtttcgc cgacatgaag ctccgccgca cgatgtacgc gctatttggc ggagccgaga
     2161 tccaggccgg cgacgagccg ttcgatcagg agttcttcat tgaggggccg gttcagctcg
     2221 ggacggcgat gctcgacgcg ga
//
LOCUS       DASPJI010000708         3250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_215414, whole genome shotgun sequence.
ACCESSION   DASPJI010000708 DASPJI010000000
VERSION     DASPJI010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215414"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VEL74_19905"
     CDS             complement(<1..211)
                     /locus_tag="VEL74_19905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64856.1"
                     /translation="MNFGAVLRTISGFLEEQRIRFAVVGGVALASYGLPRTTVDLDIV
                     VEAGAQERVILFLESLGYRTVHRSSG"
     gene            341..673
                     /locus_tag="VEL74_19910"
     CDS             341..673
                     /locus_tag="VEL74_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009019266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HYG64857.1"
                     /translation="MAGKFEIQQATDGQFTFHFKAGNGRIVIAGERYAQRRSAEAGVA
                     SVQKNAASPGRFERKTAKNGAPYFVLTATNGQVIGQSETYSSPAAMEKGIASLQKNAP
                     GARVIDLT"
     gene            complement(762..1604)
                     /locus_tag="VEL74_19915"
     CDS             complement(762..1604)
                     /locus_tag="VEL74_19915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64858.1"
                     /translation="MKKLVWMIGVGLALLAGTLEAASCNGNCRCGGNVEWACFQDCDE
                     GWSALCTDGSCGAFIASCCCQQVVLGGGLNYFCVEKTCGIQSFRSSFSSEAPLPGTEE
                     ILKLRPGTSAGQAEEWAYLEYQIGAERKVVAAKVRYTSNPLFGETLVQQRLHGQRSAL
                     AGPGTYRSFELRPPQGRPGFGDERRLAEASRLLKVPVPESAPDQGVFAWRIELNPAGD
                     VLDRTEIFSTSEGLSSGLDAPLEAFLDHSGLREAGKPLVVFLVYQIEQKRIVSVGVSN
                     YLGD"
     gene            2114..2467
                     /locus_tag="VEL74_19920"
     CDS             2114..2467
                     /locus_tag="VEL74_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64859.1"
                     /translation="MPISFADVTLDWESILAALREHPDLLSILEAERLALETDLNLIR
                     VLKSRQEAQAASRQELTQKIKAVRAHGVAQAIAIRAVAKGKIGYRNERLVHFKVAPVR
                     SRRRAPKPEEVPQAE"
     gene            2530..>3250
                     /locus_tag="VEL74_19925"
     CDS             2530..>3250
                     /locus_tag="VEL74_19925"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HYG64860.1"
                     /translation="MRITKGQFTGCLLGLATGDALGAPHEGGGLEKALWRFIGRTRDG
                     ALRWTDDTRMALDLAESLLEEDGVRPDALAKRFAGSYHWSRGYGPGTARVLRRIRRGE
                     PWQSASRAVHPDGSYGNGAAMRSPVVALFARDEAAIVEAARASALVTHGHPQAITGAV
                     LIALATGHLLRGTPAPGLFAGLRTVCRDAEIAERLRRAASWILNGEVPAPYEVAVWLG
                     NGMAAVASCATAIYIALRHLRS"
BASE COUNT          564 a         1104 c         1114 g          468 t
ORIGIN      
        1 aacccgagga acggtgaacg gtccgatagc ccagggattc caggaacagg atcaccctct
       61 cctgggcgcc ggcttcgacc acgatgtcca ggtcgaccgt cgtgcggggc aggccgtagg
      121 aggccagggc cacgccgccc acgactgcga agcggatacg ctgctcctcc aggaatccgg
      181 agatggtccg caggacggcc ccaaagttca cctccacagc atagcaaccc agggccgccg
      241 ggcgcgtata attctcccag gcatcacacg attcacgcga ccatcaagaa gccgtccgcg
      301 ccggcgggtg gtcccgcacg gcgggcggca gggagggcgt atggcaggca agttcgagat
      361 ccagcaggcg acggacgggc agttcacgtt ccacttcaag gccggaaacg ggcggatcgt
      421 catcgccggc gagcgctacg cgcagagaag gagcgccgag gcgggggtgg cgtcggtcca
      481 gaagaacgcc gcgtccccgg gccggttcga gaggaagacc gcgaagaacg gcgcgccgta
      541 cttcgtcctc acggcgacca acggccaggt gatcgggcag agcgagacgt actcctcgcc
      601 ggccgccatg gagaagggga tcgcctccct ccagaagaac gcccccggcg cgcgggtcat
      661 cgacctgacc tgagggcctg atggagtcgg cgccttatcc tccccccggg ggcggacccg
      721 aggggaggtg gcgccttgcg gcgggaaggc gaggaggggg cctaatcccc gaggtagttg
      781 ctgacgccga ccgagacgat gcgcttctgc tcgatctgat agacaaggaa gacgacgagc
      841 ggcttcccgg cttcgcgaag gccgctatgg tcgaggaacg cttccagggg tgcatccagt
      901 ccgctggaaa gtccctcgga cgtagagaag atctcggtcc ggtccagcac gtcgcccgcc
      961 gggttcagct cgatacgcca tgcgaacacg ccctggtcgg gagcggattc cggcacgggt
     1021 accttgagga gtctggacgc ctccgccagc cggcgctcgt ccccgaaacc cggccgcccc
     1081 tggggaggcc gcaactcgaa gcttcgatag gtccccggac ccgcaagggc ggaccgttgc
     1141 ccgtggagcc tctgctggac gagggtctcg ccaaacaggg gattggaggt gtaccgcacc
     1201 ttggcggcga ctaccttgcg ctcggcaccg atctgatact ccagataggc ccattcctcc
     1261 gcctgacctg cggaggtccc cggccggagc ttcaggatct cctcggtacc gggcagagga
     1321 gcttcgctcg agaaggacga cctgaaagac tgaattccac aggtcttctc gacgcagaag
     1381 tagttcaggc cgcctccgag aactacttgc tggcagcaac agctcgcaat gaaagctcca
     1441 caactgccat ccgtgcagag tgcgctccag ccctcatcac aatcctggaa acaggcccac
     1501 tcgacattgc cgccgcaccg gcagttgccg ttgcagctag cagcttccaa ggtgccggcc
     1561 aacagcgcga gtcccactcc gatcatccag actagctttt tcatggcttc gtccccgggt
     1621 ctggagttta gaatcttcca agaatcgtcg caagcggcca gaagatcgtg gcgccccctg
     1681 tttccggaat agcgggccgc gcgaggcccg cgcccggcct ggccaggccg gaggccggca
     1741 gggtgacacc cacgacaggc atgatcaggc ctggcaggag cccggtgcgg ccggaggcag
     1801 gccgcgcccg gccggtgaca ggctccctgc ggccgatgaa gggccgcgcc cggccggtgg
     1861 ccggcaaggc gcggccgagg acgggcctgt cccggccgct cgggcgcacg gccaggcctg
     1921 aaacaggcct ggcggagccg gcaacaggct gggaggccgg cctggacagc attagaaaaa
     1981 tcttggctca ccctcttgac aggccagcga acggtctcta tcgtacccct caggccgaat
     2041 atggtcaaca gagccgttga ggctctatcg ggggccgatg gaggcttccc gatcctgtga
     2101 aggagaagaa ggtatgccca tctcgtttgc cgacgtgacg ctggactggg agagcattct
     2161 cgccgccctc cgggaacatc cggacctgct gtccatcctc gaagccgaac gcctcgcgtt
     2221 ggagacggac ctgaacttga tccgggtgct caagtcgcgg caggaggcgc aggcggcctc
     2281 gcggcaggag ctgacgcaga agatcaaggc cgtgagagcc cacggcgtgg cacaggccat
     2341 cgccatccgc gcggtcgcca agggcaagat cggctatcgc aacgagcggc tggtccactt
     2401 caaggtcgct ccggttcgta gccgccgccg cgcgcccaag ccggaggagg tcccgcaggc
     2461 ggagtagtat caggaggggg aaggcgccac acgatctgtg tggcgccttt tcgatcggct
     2521 ccagatgcca tgaggatcac gaagggccag ttcaccggct gcctgctcgg gctcgccacc
     2581 ggggatgccc tcggcgcgcc ccacgagggg ggcgggctgg agaaggcgct ctggcgtttc
     2641 atcggccgca cccgggacgg cgccctgcgc tggaccgatg acacgcggat ggctctcgac
     2701 ctcgcggagt ccctgctcga ggaggacggg gtgcgtccgg acgcgctggc gaagcgcttc
     2761 gccgggagct accactggag ccgtggctat ggccccggca cggcgcgggt cctccggcgg
     2821 atccggcgtg gcgaaccctg gcagtccgcc tcgcgggccg tgcacccgga cggctcctac
     2881 ggcaacggcg ccgcgatgcg ctccccggtc gtcgcgctct tcgccaggga cgaagccgcg
     2941 atcgtcgagg cggcccgggc ctccgccctg gtgacccatg gccatccgca ggccatcacc
     3001 ggggcggtcc tcatcgccct ggcgaccggc catctgctgc gcggtacgcc ggctcccggc
     3061 ctcttcgcgg gcctccgaac ggtctgccgc gacgcggaga tcgcggagcg gctgcgacgg
     3121 gccgcctcct ggatcctcaa cggcgaggtc ccggcgccgt acgaggtggc ggtatggctc
     3181 ggcaacggca tggccgccgt ggcctcctgc gccacggcca tctacatcgc cctccggcat
     3241 ctgcgctccc
//
LOCUS       DASPJI010000709         8316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_392326, whole genome shotgun sequence.
ACCESSION   DASPJI010000709 DASPJI010000000
VERSION     DASPJI010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8316)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392326"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1643)
                     /locus_tag="VEL74_19930"
     CDS             complement(<1..1643)
                     /locus_tag="VEL74_19930"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HYG64861.1"
                     /translation="MPRRFLPLLLLSASTALAPPIPEKAIELRNLGLAQLENERAGEA
                     EASFRRLAQAVPDDPLPYANLAVAALRQQKSDEALRWIGQALSKAPGRADLLTIQGDV
                     LQWSGRMDEALTSYRKAAAAAPAHPEIQYALYRQAESTGDDAAMAESLGRLARLRPEN
                     LVVLLQSGRRAVQAGDRPGATRAFLRVRELLWQAPASAGTALEQVLGALEKGEVASAR
                     GPAARLENVLKPTPAYQQSLRELLPGIQALPVERFAQEPPPSIFGNPVTVRFQGAPLA
                     TGPTAAVAVGDFDGDERPDVARVTSSRLEIRRAGSWKPGPAASGITGLLAADLDNDGK
                     LDLLGHGANEAVLWRGQGDGSFADATAAAGLGNARGAAAAALDFDLEGDLDLVTAGSA
                     AELYRNPLQGTFEAVGREALPGPVLAGARAVVASDLDRDGDPDLAVAGDKGLFWIDNL
                     RQGNLADRTAAAGLGRTPAAAVASADLDADGLPDLIAAGQGGLALWHNRRGRFEPWRP
                     TGLPSGRFAAVAVLDADNDGRLDLAAAGPGGVVVLAQRGA"
     gene            complement(1688..2419)
                     /locus_tag="VEL74_19935"
     CDS             complement(1688..2419)
                     /locus_tag="VEL74_19935"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HYG64862.1"
                     /translation="MSQPAVTALAPPTLDFEIVYSDGEPLESHWHVQQMHFLNDLLWQ
                     VVREQRRTDVFFGANQFVYYSVEQAREVAELVAKGRKLKFRGPDVFWVNDVMPKAERP
                     AWIAWEEGGRLPDVIFELLSKSNSLRHQKEKKELYSRVFKTAEYFIYGPPRFVLEGFR
                     LAGGAYRPIAPDPAGRLWSEQLRCFVGLWHGSYQDLETTWVRLYRQDGSLISSPWELA
                     DEERSRAEAAEAELARLRALLDRQS"
     gene            complement(2537..4405)
                     /locus_tag="VEL74_19940"
     CDS             complement(2537..4405)
                     /locus_tag="VEL74_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HYG64863.1"
                     /translation="MSEPSKENLDLQDDELVPEDDAVIGRAVRRSLIALAVIAVLGAL
                     AFFLVRRPPAAGPEKAIAAAPPQTREAPASGPPEVRFTDITQQAGIDFVHTNGAYGMK
                     LLPETMGGGAAFFDFDGDGDQDLLLVDSSTWPGQSAPAERSTPRLYRNDGTGRFTDAT
                     AAAGLAGVSLYGMGPAVGDYDGDGDPDLYLTAVGEGRLLRNQGGVFEDVTRQAGVAGS
                     PDDWSTCAAFFDADNDGRLDLFVCNYVRWSREIDLKLDYRLTGIGRAYGPPNNYEGTW
                     PRFYRNRGDGTFEDVSAASGVQVANPATGKPAAKALGVAPVDADGDGWMDLLVANDTA
                     PNFFLRNQGNGRFVETGTESGLAFDRMGNATGAMGVDAAHYRNDADLAFVIGNFANEM
                     TSLYVAQGDPSLFADEAIPEGIGAPSRQTLKFGIFFFDYDLDGRLDLLQANGHLEQEI
                     NQVDPSQQYRQPAQLFWNAGPEARQTFAAVPAASMGDLAKPLVGRGAAYADIDRDGDL
                     DVFITQPGDRPLLLRNDQALGHHWLRVKLVGSGGNREAIGARVTLTAGGGITQTRQVM
                     PTRSYLSQCELPVTFGLGKTAKVDSLMIRWPGDGGEERVENVPVDREVVVEQGKGR"
     gene            complement(4410..7280)
                     /locus_tag="VEL74_19945"
     CDS             complement(4410..7280)
                     /locus_tag="VEL74_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HYG64864.1"
                     /translation="MTTPATPSVSPAAPPRKRKYLPAVGPRLKKLLFVVFGLFALLAV
                     NSTYLASTTILEWVTGRTYQSWFYMNMFLLHLVLGLLIVVPVVVFGIAHIRNAWNRPN
                     RRAVRVGYALFAAALVLLGSGIVLTRIEGVIVVKDPAVRSVAYWAHVLAPVAAIWLFV
                     LHRLAGRRIKWKVGLRWAAVAGVFAVVMLIVQAQDPRQWNVAGPKSGERYFFPSLSRT
                     ATGNFIPARTLMNDQYCAQCHADVHEGWANSAHRFSSFNNPAYLFSVRETRTQALKRD
                     GDVNASRFCAGCHDPVVFFSGKFDDPKFDDVNDPTGQAGITCSVCHSITHVNSPRGNA
                     DYTIEEPIHYPFAFSDNRTLRWVNQQLVKAKPELHKATFLKPLHRSAEFCSSCHKVHL
                     PPELNHYKWLRGQNHYDTFLLSGASGHGVASFYYPPKANESCSPCHMQPMPSNDFAAK
                     VYDDTGRLQVRDHMFPSANTAIPHLLKLPDWVNEKHRKFNEGVMRVDLFGVKEGGTID
                     GPLTAPLRPEIPTLAPGRSYLLETVVRTVKMGHPFTQGTADSNEVWMDVTVTSGGKVI
                     GRSGGYGRDGEVDPWSHFVNVYMLDRDGNRIDRRNPQDIFVPLYNHQIPPGAADVIHY
                     RLDLPPDVRGPVTVDVKLQYRKFDTRYMKLVFGPQYVNDLPVMTLATDRVTFPVAGST
                     AVVENPESPIKETWQRWNDYGIALLLKGGKAKGELRQAEEAFAQVEALGRPDGPLNLA
                     RVYIEQGTVQDRAVEALRRAAAFTDPPAPKWTIAWLSGQVNKQNGFLDEAIAEFRSLA
                     QADTAELRERGFDFSKDYRLLNELGQVLVERARQERGERRRAQRDAFLEEARGLFERT
                     LELDPENAAAHYNLHLIFKQTGDAQKAARHLAAFQTYRPDDQARDRAIAIARSKDPAA
                     NHAAEAIVIYDLRRPGAYELSGPSGPPPPKPVSGIVTSAGGG"
     gene            complement(7401..8168)
                     /locus_tag="VEL74_19950"
     CDS             complement(7401..8168)
                     /locus_tag="VEL74_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HYG64865.1"
                     /translation="MDLILHVWRQAGPADKGRIERYEAKGVNPDSSFLEMLDVVNEGL
                     AERGVEPIAFEHDCREGICGSCGFMINGVAHGGMHRTTVCQLHMRHFKDGDELWIEPW
                     RAKAFPVVKDLVVSRTAFDRIVQSGGFISVNTGSAPEANLTPVPKSLADRAFDAAECI
                     ACGACVAQCPNGAAQLFTSAKMSHLNLLPQGQPERYERALSMVEAMEREGFGACSNYT
                     ECEGVCPKGISVDFIARMNRDYLNAKGKGELKTPMKT"
BASE COUNT         1189 a         2872 c         2961 g         1294 t
ORIGIN      
        1 gccccgcgct gggcgaggac cacgacaccc cccggccccg cggcggcgag gtcgagccgg
       61 ccgtcgttgt cggcatcgag gacggcgacg gcggcgaagc ggccggacgg cagcccggtt
      121 gggcgccagg gctcgaaacg cccccggcga ttgtgccaga gggcgagccc cccctgcccg
      181 gcggcgatga gatcggggag gccgtcggcg tcgaggtcgg cgctggcgac ggctgcggcc
      241 ggcgtgcggc ccagcccggc ggcggcggtg cggtcggcga ggttgccctg ccgcaggttg
      301 tcgatccaga agagcccctt gtcccccgcc acggcgaggt cgggatcgcc gtcgcggtcg
      361 aggtcgctcg ccaccaccgc acgcgccccg gcgagcaccg gcccggggag cgcctcgcgg
      421 cccaccgcct cgaacgtccc ctggagcgga ttgcggtaga gctcggcggc gctccccgcc
      481 gtcaccaggt cgaggtcccc ttccaggtcg aagtcgagcg ccgccgccgc cgccccccgg
      541 gcgttgccga ggccggccgc ggcggtggcg tcggcgaagc tgccatcccc ctgaccgcgc
      601 cagagcacgg cctcgttggc accatgcccc aagagatcga gcttgccgtc gttgtcgagg
      661 tccgccgcga gcagcccggt gatgccggac gccgccggcc ccggcttcca ggagccggcg
      721 cggcggatct cgagacggga cgaggtgacg cgcgcgacgt ccggccgctc gtcgccgtcg
      781 aagtcgccca ccgcgacggc ggcggtcgga cccgtcgcca ggggcgcgcc ctggaagcgg
      841 acggtgacgg gattgccgaa gatcgaaggc ggaggctcct gggcgaaccg ttcgaccggc
      901 agcgcctgaa tgccggggag cagctcgcgc aggctctgct ggtaggccgg cgtcggcttg
      961 aggacgttct ccagccgcgc cgccggtccc cgcgctgagg ccacttcgcc tttttccagg
     1021 gcgccgagca cctgctccag cgccgtcccg gcggacgccg gcgcctgcca caggagctcg
     1081 cggacccgca ggaacgcccg cgtcgccccc ggccggtctc ccgcctggac cgcgcgccgc
     1141 ccggactgga gcagcacgac gaggttctcc ggccggagcc gggcgaggcg tcccagggac
     1201 tccgccatgg cggcgtcatc gccggtgctc tccgcctgcc ggtagagcgc gtactggatc
     1261 tcggggtgcg ccggagccgc cgcggcggcc ttgcgatagg aggtgagcgc ctcgtccatc
     1321 cggccgctcc actggagcac atccccctgg atcgtcagca ggtccgcccg gccgggagcc
     1381 ttggaaagcg cctgcccgat ccagcgcagc gcctcgtcgc tcttctgttg ccggagcgcg
     1441 gcgaccgcga ggttggcata cggcagcgga tcgtccggca ccgcctgtgc caggcgccgg
     1501 aagctcgcct ccgcctcgcc ggcccgctcg ttctcgagct gggcgagccc caggttgcgc
     1561 agctcgatgg ccttctcggg gatcggcggg gccaacgcgg tcgaggccga gagcaggagg
     1621 aggggaagga accggcgcgg catggagcgc cgattgtact agggggcggg ggcggcgcct
     1681 ctcgccgtca gctctgccgg tcgagcagag cgcgcaggcg ggccagctcc gcttccgccg
     1741 cctcggcccg gctgcgctcc tcgtccgcca gctcccaggg cgatgagatc aagctgccgt
     1801 cctggcgata gagacggacc caggtggtct ccaggtcctg atagctgccg tgccagagcc
     1861 cgacgaagca gcggagctgc tcgctccaca gccgccctgc cggatcgggc gcgatggggc
     1921 ggtaggcgcc gccggcaagt cggaagccct ccagcacgaa acgcggtggg ccgtagatga
     1981 agtactccgc cgtcttgaag acccgggagt acagctcctt cttctccttc tggtggcgca
     2041 acgaattcga cttcgacagc agctcgaaga tgacgtccgg cagccggccg ccttcctccc
     2101 aggcgatcca cgccggacgc tccgccttcg gcatgacgtc attgacccag aagacgtccg
     2161 gcccgcggaa cttcagcttc cggcccttgg ccaccagctc ggcgacctcc cgcgcctgct
     2221 ccaccgagta gtagacgaac tggttggcgc cgaagaagac gtcggtccgc cgctgctccc
     2281 tcaccacctg ccagagcaga tcgttcagga agtgcatctg ttgtacatgc cagtggctct
     2341 ccaaaggctc cccgtcgctg tagacgatct cgaaatccaa ggtcgggggc gcgagcgccg
     2401 tcaccgctgg ctggctcatg gctggaccct ttcggaatcg ttcctttgga gaatcttaga
     2461 ccggatcttc agtaaagggg aagagggaag gtgcgccttc cggcctccca gctggaggcc
     2521 aggataccat ggaggcctac ctccccttcc cctgctccac cacgacctcc cggtccaccg
     2581 gcacgttctc cacccgctcc tcccccccgt cccccggcca gcggatcatc agcgaatcca
     2641 ccttggccgt cttccccagc ccgaaggtga ccggcagctc gcactgcgag aggtagctgc
     2701 gggtgggcat cacctggcgg gtctgggtaa tgccgccgcc ggcggtcagc gtcacccggg
     2761 cgccaatggc ctcccggttg ccgccgctgc ccaccagctt cacccgcagc cagtggtggc
     2821 cgagcgcctg gtcgttgcgg agcagcagcg gccggtcgcc gggctgagta atgaagacgt
     2881 ccaggtcgcc gtcgcggtcg atgtcggcgt aggcggcgcc ccggccgacg agcggcttgg
     2941 cgaggtcccc catcgacgcg gcggggacgg cggcgaaggt ctggcgggct tccgggccgg
     3001 cgttccagaa gagctgcgcc ggctgccggt actgctggct gggatcgacc tggttgatct
     3061 cctgctccaa gtggccgttg gcctgcagga ggtccagccg tccgtccagg tcgtagtcga
     3121 agaagaagat gccgaacttg agcgtctggc gcgacggggc gccgatcccc tcggggatgg
     3181 cctcgtcggc gaagagcgac gggtccccct gcgcgacgta gagggaggtc atctcgttgg
     3241 cgaagttgcc gatgacgaag gcgaggtcgg cgtcgttccg gtaatgggcc gcgtccaccc
     3301 ccatggcgcc ggtagcgttg cccatccggt cgaaggcgag gccggactcg gtgccggtct
     3361 cgacaaagcg gccgttcccc tggttccgga ggaagaagtt cggcgccgtg tcgttggcca
     3421 ccagcaggtc catccagccg tcgccgtcgg cgtccaccgg cgccacgccc agcgccttgg
     3481 ccgccggctt gcccgtcgcc ggattggcta cctggacgcc gctcgccgcc gacacgtcct
     3541 cgaaggtgcc gtcgccccgg ttgcggtaga aacgcggcca ggtcccctcg taattgttcg
     3601 gcggaccgta ggcgcggccg atgccggtca gcctgtagtc gagcttgagg tcgatctcgc
     3661 gcgaccagcg gacgtagttg cagacgaaga ggtcgagccg gccgtcgttg tcggcgtcga
     3721 agaaggcggc gcaggtgctc cagtcgtcgg ggctgccggc gacaccagcc tgccgggtga
     3781 cgtcctcgaa cacgcccccc tggttgcgca gtagccgccc ctcccccacc gcggtcagat
     3841 agaggtcagg gtctccatca ccgtcgtagt cccccaccgc ggggcccatg ccgtagaggg
     3901 agacgccggc gagccccgcc gccgcggtgg cgtcggtgaa gcggccggtg ccgtcgttgc
     3961 gatagagccg cggggtggaa cgctccgctg gagccgactg cccgggccag gtgctcgaat
     4021 ccaccagcag caggtcctga tcgccgtcgc cgtcgaagtc gaagaacgcg gcgccgcccc
     4081 ccatggtttc ggggaggagc ttcatgccgt aggcgccgtt ggtgtggacg aagtcgattc
     4141 cggcctgttg cgtgatgtcc gtgaagcgga cctccggcgg gcccgaggcc ggcgcctccc
     4201 gggtctgcgg cggcgcggcc gcgatggcct tctccggccc ggccgccggg ggccggcgca
     4261 ccaggaagaa ggccagcgcg ccgaggacgg cgatcaccgc gagggcgatg agcgaccggc
     4321 ggacggcgcg gccgatgacc gcgtcgtcct cggggaccag ctcgtcatcc tggaggtcca
     4381 ggttttcctt ggagggctcg ctcatagtct cagccgccgc cggcagacgt cacgatgccg
     4441 gagaccggct tcggaggggg tggaccggac gggccggaca gctcgtaggc ccccggccgg
     4501 cgcaggtcgt agatgacgat cgcctcggcg gcatggttcg ccgccgggtc cttggaccgg
     4561 gcgatggcga tcgcgcggtc gcgcgcctgg tcgtccggcc ggtaggtctg gaaggccgcg
     4621 aggtgccgcg ccgccttctg ggcatctccg gtctgcttga agatcagatg caggttgtag
     4681 tgcgccgcgg cgttctcggg atcgagctcc agcgtccgct cgaacaggcc gcgcgcctcc
     4741 tcgaggaagg cgtcgcgctg tgcccgccgc cgctcccccc gctcctggcg ggcgcgctcc
     4801 accagcacct gccccagctc gttgagcagc ctgtaatcct ttgagaagtc gaagccgcgc
     4861 tcgcgcagct cggcggtgtc ggcctgcgcc aggctccgga attcggcgat ggcctcgtcc
     4921 aggaagccgt tctgcttgtt cacctggccc gagagccagg cgatcgtcca cttgggagcc
     4981 ggcggatcgg tgaacgccgc ggcgcggcgt agcgcctcga cggcgcggtc ctgcaccgtt
     5041 ccctgctcga tgtagacccg cgccaggttg agcgggccgt ccggccggcc gagcgcctcc
     5101 acctgcgcga aggcctcctc cgcctggcgc agctcaccct tggccttccc ccccttgagg
     5161 agcagggcga tgccgtagtc gttccaccgc tgccaggtct ccttgatcgg cgactccggg
     5221 ttctcgacca ccgccgtcga accggcgaca gggaaggtga cccggtccgt cgccagggtc
     5281 atcaccggca ggtcgttcac gtattgcggc ccgaagacga gcttcatgta gcgcgtgtcg
     5341 aacttccggt actggagctt gacgtccacc gtgaccggcc cgcgcacgtc cggcggcagg
     5401 tcgagccgat agtggatgac gtcggccgct cccggcggga tctggtggtt gtataggggc
     5461 acgaagatgt cctgcgggtt gcggcggtcg atgcggttgc cgtcgcggtc cagcatgtag
     5521 acgttgacga agtgcgacca gggatcgacc tcgccgtccc gcccgtagcc gccgctccgg
     5581 ccgatgacct tgccgccgct ggtgaccgtc acgtccatcc agacctcgtt ggagtccgcc
     5641 gtcccctggg tgaacgggtg ccccatcttc accgtgcgga ccaccgtctc cagcaggtag
     5701 gacctgcccg gcgccagcgt ggggatctca gggcgcagcg gcgcggtgag cgggccgtcg
     5761 atcgtccccc cctccttgac gccgaagagg tcgacccgca tcaccccctc gttgaacttc
     5821 ctgtgcttct cgttgaccca atccggcagc ttcagcagat gtggaatcgc cgtgttcgcc
     5881 gacgggaaca tgtggtcgcg cacctgcagc cggccggtgt cgtcgtagac cttggccgcg
     5941 aagtcgttgg acggcatcgg ctgcatgtgg cagggagagc agctctcgtt cgccttcggc
     6001 ggatagtaga agctggccac gccgtgcccg gaggcgccgg agaggaggaa ggtgtcgtag
     6061 tggttctggc cgcgcagcca cttgtagtgg ttgagctcgg gcggcaggtg caccttgtgg
     6121 caggaggagc agaactcggc cgagcggtgc agcggcttca ggaacgtcgc cttgtggagc
     6181 tccggcttcg ccttgacgag ctgctggttg acccaccgca gggtccggtt gtcactgaac
     6241 gcgaacggat agtggatcgg ctcctcgatg gtgtagtcgg cgttgccgcg cgggctgttg
     6301 acgtgggtga tcgaatggca gaccgagcag gtgatgcccg cctgccccgt tggatcgttc
     6361 acgtcgtcga acttcgggtc gtcgaacttg ccgctgaaga agaccaccgg atcgtggcag
     6421 ccggcgcaga agcgcgaggc gttgacgtcg ccgtcgcgct tcaacgcctg ggtgcgggtc
     6481 tcgcgcaccg agaagagata ggccgggttg ttgaacgagc tgaaccggtg ggcgctgttc
     6541 gcccagccct cgtggacgtc cgcgtggcac tgagcgcagt actggtcgtt catcagcgtc
     6601 ctggccggga tgaagttgcc ggtggccgtc cgggagagcg acgggaagaa gtagcgctcg
     6661 cccgacttcg gcccggcgac gttccactgc cgggggtcct gcgcctggac gatcagcatg
     6721 accaccgcga agacgccggc caccgcggcc cagcgcagcc ccaccttcca cttgatgcgc
     6781 cggccggcga ggcgatggag gacgaacagc cagatcgccg cgaccggcgc gaggacgtgg
     6841 gcccagtaag cgaccgagcg gaccgccggg tccttgacca cgatcacccc ttcgatgcgg
     6901 gtgaggacga tgccgctccc caggaggacc agggcggcgg cgaagagcgc gtagcccacc
     6961 cgcacggccc gccggttggg ccggttccag gcgttgcgga tgtgggcgat cccgaagacc
     7021 accaccggca ccacgatgag cagccccagc accaggtgga gcaggaacat attcatgtag
     7081 aaccagctct gatacgtccg cccggtgacc cattcgagta tcgtcgtgct cgccaggtag
     7141 gtcgagttga ccgccagcag cgcgaacaag ccgaagacca cgaagagcag cttcttgagc
     7201 cgcggcccca cggccgggag gtacttccgt ttccggggcg gagccgccgg gctcacgctg
     7261 ggcgtggcag gtgtcgtcat atccccttgc ctgggcgatc gcgaaagcgg aattgtaacg
     7321 cttgtgccag ggagcgggtg aaacgccgac gcccggcgcg aggccgggcg tcgaaggaaa
     7381 ggccggaggg ggagcgcggc ctaggtcttc atgggggtct tgagctcccc cttgcccttg
     7441 gcgttgaggt agtcccggtt catgcgggcg atgaagtcga cggagatccc cttggggcag
     7501 accccctcgc actcggtgta gttggagcag gcaccgaagc cctcccgctc catcgcctcc
     7561 accatggaga gggcccgctc gtagcgctcg ggctgcccct gggggaggag gttcaggtgg
     7621 gacatcttcg ccgaggtgaa gagctgcgcc gcgccgttcg ggcactgggc cacgcaggcg
     7681 ccgcaggcga tgcactcggc ggcgtcgaag gcgcggtcgg cgaggctctt gggcaccggc
     7741 gtcaggttcg cctccggcgc gctgccggtg ttgaccgaga tgaagccgcc ggactggacg
     7801 atgcggtcga aggcggtacg gctgaccacc agatccttga ccaccgggaa cgccttggcg
     7861 cgccagggct cgatccacag ctcgtcgccg tccttgaagt ggcgcatgtg gagctggcag
     7921 accgtggtgc ggtgcatgcc gccgtgggcg acgccgttga tcatgaagcc gcaggagccg
     7981 cagatgccct cgcggcagtc gtgctcgaag gcgatgggct cgacgccccg ctccgccagc
     8041 ccctcgttga cgacgtccag catttcgagg aacgagctgt cggggttgac gcccttggcc
     8101 tcgtaccgct cgatccgccc cttgtcggcg gggccggcct ggcgccagac gtgaaggatg
     8161 aggtccatta cttgtagctc ctttgcgtgg gcttcacgaa ctcgaactcc agcggctcct
     8221 tgtgccgggt gggcgtcttg ccggccccgt tccactccca ggccgagacg aagctgtagt
     8281 cggcgtcgtt gcgcagcgcc tccccctcgg gggtct
//
LOCUS       DASPJI010000710         9164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_677828, whole genome shotgun sequence.
ACCESSION   DASPJI010000710 DASPJI010000000
VERSION     DASPJI010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9164)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9164
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677828"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VEL74_19955"
     CDS             <1..506
                     /locus_tag="VEL74_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HYG64866.1"
                     /translation="IVQHMPAGFTRAFAERLNAYLPLQVREATHQEPLLPGTVYIAPG
                     GLHLRLARERGQLRAMLSTEPAKAVHRPSVDVLFNSAGAVVGSRAVAAILTGMGQDGA
                     EGMLDLARAGVHTIAQDEASCVVYGMPRAALAAGGVTETVPLSRIGERLRELLLGTAE
                     AASGAEN"
     gene            543..944
                     /locus_tag="VEL74_19960"
     CDS             543..944
                     /locus_tag="VEL74_19960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG64867.1"
                     /translation="MQPNKVLVIDDSKLIHKMFTVLLPKSNLVHAMDGREALQRLAEH
                     PDVELMFLDINMPNMNGLEFLQQVKADAALAHIPVIIVSTEGKEQDTIRGLQAGAAAY
                     VKKPFRNDTILDLVRRVLGGGGLAMEEAVSQ"
     gene            complement(986..2119)
                     /locus_tag="VEL74_19965"
     CDS             complement(986..2119)
                     /locus_tag="VEL74_19965"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HYG64868.1"
                     /translation="MNRLSRLTAALTALAATAVLATACSRAPEKPQEYKIGVVLALTG
                     RGATYGQESLNGIQLAVDEINEEAKKNGEPQVRIVVEDSQSEGPKTVEAFRRLIDQEK
                     IFVAVGFTLSDEALSAAPLAEQRKVILFSPSASSDDLKNAGEYVFRNRESAALHAETV
                     AKAAVQRFGRKTILVLHSNSANGISYRDTFVNSVARMGSPIPVTIGYDEGKSDYRQEI
                     EELQAKNPDAVYLVGLDKELALIVKQAYEMGFKPQLFASAGAMSPRFLETAGAEAEGL
                     ICAGSPFNLESKEPHVEQFVRKYRERFGMEPEYFSATSYDAIRIIAGMMKEGVMDSDA
                     MKARLLTIKDYPGVGGSTTFDEYGEVTKPMDLVQVKGGKFQRL"
     gene            complement(2315..2914)
                     /locus_tag="VEL74_19970"
     CDS             complement(2315..2914)
                     /locus_tag="VEL74_19970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HYG64869.1"
                     /translation="MDRNDAWQLMTRHVEAQGLRRHMLAVEAAMRWYAGRLGEDSEAW
                     GLAGLLHDFDWEIHPTLEQHPRDGAPILREHGADENVVRAILSHNTEGTGVERERPID
                     FALLACDEVTGLIIAGALVRPSKDVREVELKSIKKRWKERTFAAGVDREHVEAATADF
                     SRVCFGGQLELWQHVDNVLTAMKGIAAELDLDGRLASQG"
     gene            3033..3554
                     /locus_tag="VEL74_19975"
     CDS             3033..3554
                     /locus_tag="VEL74_19975"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HYG64870.1"
                     /translation="MRNVTLITTGGTIEKTYDETKGDLVNRGSIVRRMLSELRLEETE
                     VNVFELMSKDSLEMTDADRARVLEAVRVCGGDAGNRGEVTGIIVLHGTDTLALTGERI
                     FQEVPEPRVPIVLTGAMRPFEMKQSDALQNLTEAIFATGVLPPGVYCVAHGRVLRFPG
                     VRKDTDRGTFVHD"
     gene            3568..3963
                     /locus_tag="VEL74_19980"
     CDS             3568..3963
                     /locus_tag="VEL74_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HYG64871.1"
                     /translation="MAETTDNPTKTMEQFRAYRERMNERIMAADHLGIKRFFNLDSAA
                     YRDGALDGRVKELLGLAASAVLRCNDCIDYHLDQCVEAGWSDEELHDALNVALVVGGS
                     IVIPHLRHAFDTIDQLRAERAAAPAPAAE"
     gene            4088..4312
                     /locus_tag="VEL74_19985"
     CDS             4088..4312
                     /locus_tag="VEL74_19985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64872.1"
                     /translation="MKNWLRLALLSAFALLACLATFPEPAAARPLCENIHGRTCSSTL
                     TPACAWGDGVQARCVCESGRWNCIYEDGLG"
     gene            4716..5366
                     /locus_tag="VEL74_19990"
     CDS             4716..5366
                     /locus_tag="VEL74_19990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64873.1"
                     /translation="MRSDQPLRIEVEVEVAHRGSAPLHLNRNKRHGRAVVAADADGVK
                     MIEQRWLGRSWLASLWKDKPDDDEPMLDELEAENLVDPVGMIDLLLTDATLVSDEAAT
                     WQDQPARLLVIRPGLPVGEREEEAPAPSADVESEPGPLTLDAKIWLDENGVPLALERS
                     MELGLGVVKATGDESFTFQQSEGRLLVSSAELSSTGQALAVLRSRDDKKLKVTGIR"
     gene            complement(5350..6384)
                     /locus_tag="VEL74_19995"
     CDS             complement(5350..6384)
                     /locus_tag="VEL74_19995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013557635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HYG64874.1"
                     /translation="MKNLVDMPLSLSVLDLAPIASGTTPSQALRQTVDLARLTERLGY
                     TRYWFAEHHSIPSVASSAPEVLIGHVASATERIRVGSGGIMLPNHVPLRVAETFHTLE
                     ALHPGRIDLGLGRAPGTDPVTSQALRPFPADQFPAQLTELLALSRGEIPEDHPLRTVR
                     VIPDDVELPPIWILGSSGASARLAGSLGLGYSFARHFSLTPAEPALEAYFESFRPSSQ
                     FPEPSAILALAVVCADSDERARHLAWTMDLSWVRLRSGQLGPLASPEEAMAYPYTPEE
                     RALAGAYRKLLVLGDPESVRSQIAALAAEAGVQEVMISTNIYSHEERLRSYELVAQAF
                     GLPGGLSGSR"
     gene            6493..8154
                     /locus_tag="VEL74_20000"
     CDS             6493..8154
                     /locus_tag="VEL74_20000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019865276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYG64875.1"
                     /translation="MLRLSELRLPLEHTEDELRAAILRELGIERQDLTAYSVFQRSYD
                     ARKRGQIALIYHLDVETPREGEVLRRLRGNPHVGPTPDTTYRLVARAPESLPSRPVVI
                     GFGPCGLFAGLLLAEMGFRPIILERGKQVRERTVDTFGLWRKRNLDPESNVQFGEGGA
                     GTFSDGKLYSQIKDPRHLGRKVLTEFVEAGAPPEILYVSKPHIGTFRLVKMVESLRAK
                     IESLGGEIRFQSRVEDLIIEHGKVRGLVLAGGERLAADHVVLAVGHSARDTFEMLFRR
                     GVYIEAKPFSIGFRIEHPQSLIDQCRFGPQAGHPILGAADYKLVHHCRNGRSVYSFCM
                     CPGGTVVAAASEPGRVVTNGMSQYSRDERNANSGIVVGITPEDYPGGPLAGIDFQRHW
                     EARAFELGGGTYDAPAQLVGDFLAGRPSTDLGSVLPSYRPGVRMTDLSTSLPDYAIAA
                     IREALPAFERKIRGFAMEDALLTGVETRTSSPVRIKRNEELQSLNTEGLYPAGEGAGY
                     AGGILSAAVDGIKVAEAVALSITGSAALAGPGNVASYKVPLESTA"
BASE COUNT         1424 a         3101 c         3201 g         1438 t
ORIGIN      
        1 tgatcgtcca gcacatgccc gccggcttca cccgcgcctt cgccgagcgc ctgaacgcct
       61 acctgccgct ccaggtgcgg gaggcgacgc accaggagcc gctgctgccg ggcacggtct
      121 acatcgcgcc gggcggtctc cacctccggt tggcgcgcga gcgcgggcag ctccgcgcga
      181 tgctgtccac cgagccggcg aaggcggtcc atcgcccctc ggtggacgtg ctgttcaatt
      241 ccgccggcgc cgtggtgggg agccgcgccg tggccgcgat cctcaccggc atggggcagg
      301 acggggccga ggggatgctc gacctcgccc gggcgggggt ccacacaatc gcccaggacg
      361 aagcgtcctg cgtcgtctat ggcatgccgc gggcggccct cgccgccggt ggagtcaccg
      421 agaccgtgcc gctgtcgcgc atcggcgaac gcctgcgcga gctgctgctg gggacggccg
      481 aggccgcctc cggagccgag aattaggatt ctatacgccg gaagatccgg ggggatttcc
      541 atatgcagcc gaacaaagtc ctggtcatcg acgactcgaa gctgatccac aaaatgttca
      601 ccgtcctgct gcccaagtcg aacctggtcc acgccatgga cgggcgcgag gccctgcagc
      661 ggctggccga gcaccccgac gtcgagctga tgttcctcga catcaacatg ccgaacatga
      721 acgggctcga gttcctccag caggtgaagg cggacgcggc gcttgcccac atccccgtca
      781 tcatcgtgag caccgagggg aaagagcagg acaccatccg cgggctccag gcgggggccg
      841 cggcctacgt caagaagccg ttccgcaacg acaccatcct cgatctggtg cggcgggtgc
      901 tgggcggggg tgggctggcg atggaggaag ctgtctccca gtaggaggag acagctccct
      961 ccggcctttc atcggcgggc gccgctcaga ggcgctggaa cttgccgccc ttgacctgca
     1021 cgaggtccat gggcttggta acctcgccgt actcgtcgaa cgtcgtcgag ccgcccaccc
     1081 cggggtagtc cttgatggtg aggagccgag ccttcatggc gtcggagtcc atcaccccct
     1141 ccttcatcat gccggcgatg atgcggatgg cgtcatagga ggtggcggag aagtactccg
     1201 gctccatgcc gaagcgctcc ctgtacttcc gcacgaactg ctcgacgtgc ggctccttgg
     1261 actcgaggtt gaagggggag ccggcgcaga tcagcccctc ggcctcggcg cccgcggtct
     1321 ccaggaagcg cgggctcatg gcgccggcgc tggcgaagag ctgcggtttg aaccccatct
     1381 catacgcctg cttgacgatc agcgccagct ccttgtccag ccccacgaga tagacggcgt
     1441 cggggttctt cgcctgcagc tcctcgatct cctggcggta gtccgacttg ccctcgtcgt
     1501 agccgatggt gacggggatg ggggagccca tgcgagccac ggagttgacg aaggtatcgc
     1561 ggtaggagat gccgttggcg gagttggagt ggagcaccag gatggtcttc ctgccgaagc
     1621 gctggacggc ggccttggcc acggtttcgg cgtgcagcgc ggcgctctcg cggttgcgga
     1681 agacgtactc gccggcgttc ttgaggtcgt cgctgctggc ggagggggag aagaggatga
     1741 ccttgcgctg ctcggcgaga ggtgcggcgg agagggcctc gtcgctcagg gtgaagccca
     1801 cggcgacgaa gatcttctcc tgatcgatca gcctgcggaa cgcctccacc gtcttggggc
     1861 cttcggactg ggagtcctcg accacgatgc ggacctgggg ctcaccgttc ttcttggcct
     1921 cttcgttgat ctcgtcgacg gcgagctgga tgccgttgag cgactcctgg ccataagtgg
     1981 caccccggcc ggtgagggcg aggaccacgc cgatcttgta ctcctggggc ttctcgggcg
     2041 ccctgctgca cgccgtcgcc aggaccgcgg tggcggccaa ggcggtcagc gcggcggtca
     2101 gcctggacag tcgattcatt tcggaaacct ccggtcttct gcggtactcc gcggcttgcg
     2161 agccggcgct cttcccgggt tctcctccgg cgtacacagg cgcactgccg gcgttccgga
     2221 ggaccgttcg agctctgggg gctgcgtgtt ggcccattct agcccaagcc ggaggggtgt
     2281 caacggccaa ggcaagcggg acagcgggga cggctcagcc ctgggaggcc aggcggccgt
     2341 ccaggtccag ctcggcagcg atccccttca tcgccgtgag gacgttgtcg acgtgctgcc
     2401 agagctcgag ctggccgccg aagcagacgc ggctgaagtc ggcggtggcg gcctcgacgt
     2461 gctcgcggtc gacgccggcc gcgaaggtcc gctccttcca ccgcttcttg atcgacttga
     2521 gctccacctc gcgcacgtcc ttgctcgggc gcaccagggc gccggcgatg atcaggccgg
     2581 tcacctcgtc gcaggcgagg agggcgaagt cgatggggcg ctcgcgctcg acgccggtgc
     2641 cctcggtgtt gtgggagagg atggcgcgga cgacgttctc gtcggcgccg tgctcgcgca
     2701 ggatgggggc gccgtccctg gggtgctgct cgagggtggg gtggatctcc cagtcgaagt
     2761 cgtggagcag tccggcgagc ccccaggctt cggagtcctc gcccaggcgc ccggcatacc
     2821 agcgcatcgc ggcctcgacg gcgagcatgt gccggcgcag gccctgggcc tccacgtgac
     2881 gggtcatcaa ctgccaggca tcgttgcggt ccatgaggtc tcctccggcg cagcaggata
     2941 tcccggagag ggagccgcgg tcgatcctgc catcaggatc tctggctgcc agcgccgctc
     3001 cgggagggca aggtgcgata gagttccccg ccgtgcgcaa cgtcaccctg atcaccaccg
     3061 gcggcaccat cgagaagacg tacgacgaga ccaagggcga tctggtcaac cgcgggtcca
     3121 tcgtgcggcg gatgctctcc gagctgcggc tcgaggagac ggaggtcaac gtcttcgagc
     3181 tgatgagcaa ggacagcctg gagatgaccg acgccgaccg cgcccgcgtg ctggaggcgg
     3241 tgcgggtctg cggcggcgat gccggaaacc gcggcgaggt caccggcatc atcgtcctcc
     3301 atggcaccga cacgctggcg ctcaccggcg agcgcatctt ccaggaggtc cccgagccgc
     3361 gcgtgcccat cgtcctcacc ggagccatgc ggccgttcga gatgaagcag tccgacgcgc
     3421 tccagaacct gaccgaggcg atcttcgcca ccggcgtgct ccccccgggc gtctattgcg
     3481 tcgcccacgg ccgcgtcctg cgcttccccg gcgtgcgcaa ggacaccgac cgcggcacct
     3541 tcgtccacga ctgaggagga tctccccatg gccgaaacca ccgacaaccc gaccaagacg
     3601 atggagcagt tccgggccta ccgcgagcgc atgaacgagc gcatcatggc ggccgatcac
     3661 ctggggatca agcgcttctt caacctggac agcgccgcct accgcgacgg cgcgctggac
     3721 ggccgcgtca aggagctgct cgggctggcc gcttcggccg tcctgcgctg caacgactgc
     3781 atcgattatc acctggacca gtgcgtcgag gccggttgga gcgacgagga gctccacgac
     3841 gccctgaacg tcgcccttgt ggtgggaggc agcatcgtca tcccccatct gcgccacgcc
     3901 ttcgacacca tcgaccagct ccgcgccgag cgcgccgcgg ccccggctcc ggcggcggag
     3961 tagcctccac tcgacggtgc ccgcggttcc caaaattcct tgtcgccggt gtgaggggaa
     4021 aggcggatcc cctacgttct acccggcgtg cggtgagcaa acgcacgcaa cgacaaggag
     4081 atgttgaatg aagaattggt tgcgactcgc tctgttgtcc gccttcgccc tcctggcctg
     4141 cctggccacc ttccccgagc ccgctgcggc aagacccctc tgcgaaaaca ttcacggccg
     4201 tacctgttcc tcgacgctca ctcccgcgtg cgcgtgggga gacggtgtgc aggcgcgctg
     4261 cgtctgcgag tccggccgct ggaactgcat ctatgaggac ggtctcggct agcccgttcg
     4321 cataggtggt tgccggatcg gcccggtggg acgccaccgg gccggtctct ccaccaccag
     4381 tcggtacgaa cccgcttctc tctttggaaa tggaatgtcc gcggatgaca cctggggggc
     4441 gagcacctga gacggtgctt catcgtcctc cggagaggcc ggccctgtgc tgttctggag
     4501 gactccggaa tcgccttttc cccttgcgat ccccggggcg aaggtaggca ttctgattgc
     4561 aactcctgga ttcagaagga gtcgagtccg tcatccaaga gaggagaaag ctcgtgagga
     4621 cacaagcttt gaggtggatc gtggcaggcg gccttttggt ttcgagccat ctggcggccg
     4681 agccattgag tgatctgaag acgcggctgg cgacgatgag aagtgatcag ccattgcgca
     4741 tcgaggtcga ggtcgaggtg gcgcaccggg gatcggcccc tctgcacctg aaccgcaaca
     4801 agaggcacgg ccgggcggtc gtcgccgccg atgccgacgg tgtgaagatg atcgagcagc
     4861 gctggctggg ccggtcctgg ttggcttcgc tctggaaaga caagccggat gacgacgagc
     4921 ccatgctcga cgagctcgaa gccgaaaatc tggtcgatcc ggtgggtatg atcgatctgc
     4981 tcctgaccga cgccaccctg gtgagcgacg aagcggcgac ctggcaggac cagccggccc
     5041 ggctcctggt gatccgcccc ggcttacccg tgggggagcg tgaggaggag gcgcccgcgc
     5101 cgtctgcgga cgttgagagc gagcccggac cgttgactct cgacgcgaag atctggttgg
     5161 acgagaacgg cgtccctctg gccctggagc gctcgatgga gctcgggctc ggggtcgtga
     5221 aggcgaccgg tgatgaatcg ttcacatttc agcagtccga ggggcggctg ctcgtctcgt
     5281 ccgccgagtt gagctccacg ggacaggcgc tggccgtgct ccgctccaga gacgacaaga
     5341 agttgaaggt caccgggatc cgctgagccc gcccggcagc ccgaacgcct gcgccaccag
     5401 ctcataggag cgcaggcgtt cctcgtggct gtagatgttc gtcgagatca tcacttcctg
     5461 gacgccggcc tccgccgcga gcgccgcgat ctgagagcgg acgctctccg ggtcaccgag
     5521 gacgaggagc ttccggtagg cgcctgcgag cgcgcgctcc tccggcgtgt agggataggc
     5581 catggcctcc tccgggctcg ccagcggacc gagctgtccg ctccgcagcc gcacccacga
     5641 gaggtccatc gtccaggcga ggtgccgcgc ccgctcgtcg gagtcggcgc agaccacggc
     5701 gagcgccagg atcgcgctcg gctccgggaa ttggctggag ggacggaagc tctcgaaata
     5761 agcctcgagc gccggctccg cgggcgtcaa gctgaagtgc cgggcgaagc tgtagcccag
     5821 gccgagcgag ccggcgaggc gggcgctcgc gccgctcgac cccaggatcc agatcggcgg
     5881 cagctccacg tcgtccggga tcacccgtac ggtgcggagc gggtggtcct ccgggatctc
     5941 tccccgcgac agcgccagca attcggtcag ttgcgcgggg aactggtcgg cgggaaacgg
     6001 gcgcagcgcc tgcgaggtga ccggatcggt gcccggcgcc cggccgagcc ccaggtcaat
     6061 gcggcccggg tgcagcgcct ccagcgtgtg gaacgtctcg gccacccgca gcggcacgtg
     6121 gttgggcagc atgatgccgc cggagccgac acggatccgc tcggtcgccg acgccacgtg
     6181 cccgatcagc acctcgggcg ccgagctggc gacgctgggg atgctgtggt gctcggcgaa
     6241 ccagtagcgc gtgtagccga ggcgctcggt caggcgagcc aggtccaccg tctggcgcag
     6301 cgcctgggac ggagtggtgc cggaagcgat gggggcgagg tccagtacgg agagcgagag
     6361 aggcatgtcc actaggttct tcaaggactg cgcccgcggt tgcggaatac ccgccggacg
     6421 ctctaatgtt cccagagtct ctgccggctt tccaccgccg ggatgccgac ccgggaccca
     6481 ggatacagga tcatgctgcg actctcagag ctgaggcttc ccctcgaaca tacggaggac
     6541 gagttgcggg ccgccatcct ccgggagctc ggcatcgagc ggcaggacct gaccgcttac
     6601 tcggtcttcc agcgcagcta tgacgcgcgc aagcgcggcc agatcgccct catctatcat
     6661 ctcgacgtcg agaccccccg cgagggcgag gtcctgcggc gcctgcgtgg caacccgcac
     6721 gtcgggccga cgccggacac cacctatcgg ctcgtggccc gggcgccgga gagcctgcct
     6781 tcgcggccgg tcgtgatcgg cttcggcccg tgcggcctct tcgcgggcct gctgctggcc
     6841 gagatggggt tccggccgat catcctggag cgcggcaagc aggtccgcga gcgcacggtc
     6901 gacaccttcg ggctctggcg caagcggaac ctcgatccgg aatccaacgt gcagttcggc
     6961 gagggcggcg ccggcacctt ctccgacggc aagctctaca gccagatcaa ggacccgcgg
     7021 cacctcggcc gcaaggtgct gacggagttc gtcgaggccg gcgccccgcc cgagatcctc
     7081 tacgtcagca agccccatat cggcaccttc aggctggtga agatggtgga gagcctgcgc
     7141 gccaagatcg agtccctggg aggagagatc cgcttccaga gccgggtcga ggatctgatc
     7201 atcgagcacg ggaaggtacg ggggctggtc ctcgccggtg gcgagcgcct ggcggccgat
     7261 cacgtggtcc tcgcggtggg acacagcgcc cgggacactt tcgaaatgct tttccggcgc
     7321 ggcgtctaca tcgaggccaa gccgttttcg atcggatttc gcatcgagca tccgcaatcg
     7381 ctcatcgacc agtgccgctt cggcccgcag gcggggcacc cgatcctggg cgccgccgat
     7441 tacaagctgg tccaccattg ccgcaacggc cggtcggtct acagcttctg catgtgtccg
     7501 gggggcacgg tggtggccgc ggcttcggag ccggggcggg tggtcaccaa cggcatgagc
     7561 cagtactcgc gcgacgagag gaacgccaac agcggcatcg tcgtcgggat cacaccggag
     7621 gactatcccg gcggtccgct ggccggcatc gacttccagc gccactggga ggcgcgcgcg
     7681 ttcgagctgg ggggcggcac ctacgacgcg ccggcgcagc tcgtcggcga cttcctcgcc
     7741 ggccggccat ccacggacct tggctcggtc ctgccgtcgt accggccggg cgtgcgcatg
     7801 accgacctca gcacgagcct gccagactac gcgatcgccg cgatccgtga agcgctcccc
     7861 gctttcgaac gcaagatccg cggcttcgcc atggaggacg ccctgctgac cggtgtcgag
     7921 acccggacct cctcgccggt gcggatcaag cgcaacgagg agctccagag cctcaacacc
     7981 gagggcctct atcccgcggg ggagggggcc ggctatgctg gcggcatcct ctccgccgcg
     8041 gtcgatggca tcaaggtggc ggaggcggtg gcgttgagca tcacgggctc cgccgcgctc
     8101 gccggcccgg ggaacgtcgc ctcgtacaag gtgcctctcg agagcacggc gtagaagcgg
     8161 ccggtgacga acgggtccgc caggagatac ccgatggtgc tcgtggtgag gtccgggatc
     8221 cccgcgttgg cccgcctcca ggtgaggccg ccgtccgtgc tgcgcacgat ggccgtgggg
     8281 gtgccgccgt agaggatctg cgggttgcgc gggtcgacca ccaccacgct gaccaccctc
     8341 gacagccgcg tccagctccg cccgccgttc gtgccgcgca ccagccaccc ctcgggatcg
     8401 aagccgtaga ccgtgccggg gcgcgaagga tcgagcgcca gccgccggac cgccggcctt
     8461 tcctgccagt tgacgccgcc gtcgcggctc acgtagagcc gcgcctcgga cctgcccgag
     8521 ccccagttca ggcgggcggc ggcatagacc tgcgccggat tccccggctc ggtcaccaac
     8581 tcgtcgacga cgagagtcgt cctcccgatg ttgggcagct ctacgtggca gggaagggct
     8641 gggcgccagc gcgcgccgcc gttgtcggta cggagcacgc cgcacgcggc cggcgccgcc
     8701 agaatccttc cggggccggt cgcggcgagg cggcggacgc cggccccatg gacctccctc
     8761 caggtgacgc cgccgtcggc gctcctgaag aggctctgcg gcgtcgctac atagaggatg
     8821 ttcgccgggg acgccggatc gaaggcgacg tcgagcagct cccggtctcc caggccgcgc
     8881 gcgaagcggt cccaggtctg gccgccgtcg atgctgacga agctctggcc gcggccgccg
     8941 ccgacgaagg catagaggac gcccggccgc gccgggtgga gccgcacgaa gagctcggcg
     9001 tcccacagcc cggcctggaa cccgaagcgc cagtgatcgc cgccgtcccg gctgcggccg
     9061 acgccgatct ggagggcgcc gatgtacagg ctctgcggcc gcgtggggtc gatctcgatc
     9121 gtcgtcgcct gcagcgggct ggaaggcggc ccggaccgtc cgcc
//
LOCUS       DASPJI010000711         3958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_613067, whole genome shotgun sequence.
ACCESSION   DASPJI010000711 DASPJI010000000
VERSION     DASPJI010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613067"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..2369)
                     /locus_tag="VEL74_20005"
     CDS             complement(75..2369)
                     /locus_tag="VEL74_20005"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYG64876.1"
                     /translation="MSVLDLPASLPPPPPPRPPRESILREMREDLAAGGGVWVLSGPS
                     GIGKTHLARRLQAELAPTTQTVWVRGAAWGRPFQPLREAAESLRLWPLEEPALPRELA
                     GVPAEMRQRETWISLFQKRGQDGPSLVLCCDDLESFDAPSWECVREMLRRRERLTVSL
                     LLLVRPGPSRLALDALSKERRFEVPPLSAQEVAALCEERLGGPLGTAETHSRWLHSVT
                     DGNPRHLLELLDFLRGTERLVPSGTGWRRAPEAGMEVPCFEELVRRRLEAALAREKPE
                     VRSWIERGAVLGSRMDVPPSIAPALERVAERTGYLERTDESPASFRISDQRTREAIQV
                     LCEDRRPTLDEGDPRLATLREEEGKWRQAAEETAVVASEALQDEMPSEAARLALWMDR
                     LLGRAGEEPESPGRLAAAEIRAEALLDLGGCREIVDQLGPRAGGALRAGRSRLLYLLG
                     AASVRLPSILDIAQGEVWLGQAQQCLAASDPSLTARIQLELARVHGEMNPREARRRFS
                     KARELARKAKDVPLQIFLARRSRLFLAPGVARLCLEQGLKQASESCLELEEALCHNNL
                     AVVFSELALLGVAEFHARACVEILEAYGPWGLAVPLSNLALVAIARGDWDRGEALLER
                     ALAIAGDAGTNVMVRCNQAMVRATLGAPDLAVRDLRRLVPEAGQDVVLRAILFNLARA
                     LLEAGQVAEALAAATVPPWLPGDEDLARAALASLRLQAFRQSGAKGRPEKGLLRQDRL
                     LALSAKPQAWMYRSLWAPGYLLFL"
     gene            complement(2470..3090)
                     /locus_tag="VEL74_20010"
     CDS             complement(2470..3090)
                     /locus_tag="VEL74_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64877.1"
                     /translation="MPEVTTKELSERLGVDRAELHKLCREYGPCLEVRYGPRGTYLWS
                     ESAVEVVRGLFAPGNPRLKIERTKEVESYEGALQELRRHIIESRRLTQSLEWVYSHLA
                     AHPPAVTGFLYTLPHPDFRLASPLGALIVPSGARKFRATLVDLDLSAEGRTQIEALGG
                     LRKELLMAYLRHARQPESDPERWRVFQRLVVAGPQRTAPVPTGKAD"
     gene            complement(3078..3341)
                     /locus_tag="VEL74_20015"
     CDS             complement(3078..3341)
                     /locus_tag="VEL74_20015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64878.1"
                     /translation="MDNSIVYRVLTSFAGCIHRGEDREAAHRAYGEAVQQVQRAGDGS
                     VTLMSGSAVVERFQLAQKRRTTWLTRLLGRRRRAEEQEAACPR"
     gene            3605..>3958
                     /locus_tag="VEL74_20020"
     CDS             3605..>3958
                     /locus_tag="VEL74_20020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG64879.1"
                     /translation="MAKDTNEEVLATERKISLLGIALDLLMRTNGWTVTGLAKAAGVS
                     PKTISSYLWEEALTRERFDELARLMGLSRVKVERAHRAAALVVPPPPAAWSPVEPTPE
                     ERRIHEEAAVLAGCEV"
BASE COUNT          597 a         1428 c         1353 g          580 t
ORIGIN      
        1 cgtcgtgatt gagggcggtg gccagcctcc aggtcaggtg ctccatcgtc tcgtgcgcca
       61 tgccgcgcct ccatctagag gaagaggaga tatccaggcg cccagagcga ccggtacatc
      121 caggcttgcg gcttggcgct gagcgccagg agacggtcct ggcgcagcaa ccccttctcg
      181 ggccggcctt tggcgccgct ctgccggaag gcctggagcc gcaggctcgc cagggccgcg
      241 cgggcgaggt cctcgtcgcc cggcagccag gggggcacgg tggcggcggc aagcgcctcc
      301 gccacctggc ccgcctccag cagcgcccgg gcgagattga agaggatggc ccggaggacg
      361 acgtcctgcc cggcctccgg cacgaggcgg cgcaggtcgc ggaccgccaa gtccggggct
      421 cccagcgtgg cgcgcaccat ggcctggttg cagcgcacca tgacgttcgt cccggcgtct
      481 ccggcgatgg ccagtgcccg ttcgagcagt gcctcgccgc ggtcccagtc cccgcgggcg
      541 atcgcgacga gcgccaggtt gctgagcggc acggcgagtc cccagggccc ataggcctcc
      601 aggatctcca cgcaggcgcg ggcgtgaaac tcggcgacgc ccagcagggc cagctcggag
      661 aacaccacgg ccaggttgtt gtggcacagc gcctcctcca gctcgaggca ggactcgctc
      721 gcctgtttca gcccctgctc caggcaaagc cgggcgacgc cgggggccag gaacaggcgg
      781 ctccgccgcg ccagaaagat ctggagcggc acgtccttgg cctttcgtgc cagctcccgg
      841 gccttcgaga agcgacgtcg tgcttcccgg gggttcatct cgccatggac ccgcgccagc
      901 tccagttgga tgcgggcggt cagggacgga tcgctggccg ccagacactg ctgggcctgc
      961 ccgagccaga cctcaccctg ggcgatgtcg agaatcgagg gcaggcggac gctcgcggct
     1021 cccagcagat agaggaggcg ggaacgtccg gcccgcaggg cgccgccggc ccgcgggccg
     1081 agttggtcca cgatctcacg gcagccgccg aggtccagca gggcctcggc ccggatctcc
     1141 gcggcggcga gccggcccgg gctctccggc tcctccccgg cccggcccag gagccggtcc
     1201 atccacagcg ccaagcgggc ggcctccgaa ggcatctcat cctggagggc ctccgaggcg
     1261 acgacggcgg tctcctccgc tgcctggcgc cacttcccct cctcctcccg tagggtggcg
     1321 agccgcgggt ccccttcgtc cagcgtgggc cggcggtcct cgcagagcac ctggatcgcc
     1381 tcccgggtcc gctggtcgga gatacggaac gacgccggac tttcgtccgt gcgctccagg
     1441 tagccggtcc gctcggcgac gcgctccagg gccggcgcga tggagggcgg cacgtccatc
     1501 ctggagccga ggacggcgcc gcgctcgatc caggagcgca cctccggctt ctcgcgggcc
     1561 agcgccgcct ccagccgccg ccgcaccagc tcctcgaaac agggcacctc catcccagcc
     1621 tccggagccc gccgccatcc cgtgccactc ggcaccagcc gttccgtgcc gcgcaggaaa
     1681 tcgaggagct ccagcagatg gcggggattg ccatcggtca ccgagtggag ccaacgcgag
     1741 tgggtctccg ccgtgccgag cgggcctccc aaccgctcct cgcagagcgc cgccacctcc
     1801 tgcgcggaca gcggcggcac ctcgaaacgc cgttctttgg aaagggcgtc cagcgccagc
     1861 ctcgagggcc cggggcggac cagcaggagc agcgagaccg tgagccgctc ccggcgccgc
     1921 agcatctccc ggacgcactc ccaggagggg gcatcgaagc tctccaggtc atcgcagcag
     1981 agcaccaggg acggtccgtc ctggccacgc ttctggaaca gggagatcca cgtctcgcgc
     2041 tggcgcatct cggccggcac tccggccagc tcccgcggca gcgccggctc ctccagtggc
     2101 cacagccgga gcgactcggc cgcctcgcgc aggggctgga acggcctccc ccaggcggcg
     2161 ccccgcaccc agaccgtctg ggtcgtgggc gccagctccg cctggagccg ccgcgccagg
     2221 tgggtcttgc cgatgccgga aggcccgctg agcacccaca cgccgccgcc cgcggcgagg
     2281 tcctcccgca tctcgcgcag gatcgactcg cggggcggtc tgggaggagg gggaggcgga
     2341 agggacgccg ggaggtcgag aacgctcatc gtcggtagaa aaaagagggg actacccctt
     2401 ggaggaaggt gatgcagcgg ggtagtcccc tgaaaagccg ccgtgaccgg ccttcttcat
     2461 ggttttgtct cagtccgcct tccctgtcgg taccggcgcc gtccgctgcg gcccggccac
     2521 caccagacgc tggaagactc gccatcgctc cgggtcgctc tccggctgcc gggcatgccg
     2581 caggtaggcc atcaacagct cctttctcag cccgccgagc gcctcgatct gggtccgccc
     2641 ctcggccgac aggtccagat cgaccagcgt ggcgcggaac ttcctcgcgc cggaggggac
     2701 gatgagcgct cccagcgggg aggccaaccg gaaatcggga tgaggcaagg tgtagagaaa
     2761 ccctgtgacc gctggcggat gggccgccag gtgggagtag acccactcca gagattgggt
     2821 cagcctcctg gattcgatga tgtgccgcct cagctcctga agagcgcctt cgtagctctc
     2881 cacctctttc gtcctctcga tcttgagacg aggatttccc ggcgcgaaga ggccgcgcac
     2941 aacctcgacg gcggactcgc tccacaggta ggtgcctcgc gggccgtagc gcacctccag
     3001 acagggacca tactcacggc agagcttgtg cagctccgct cgatcgacgc ccagccgctc
     3061 gctcagctcc ttggtcgtca cctcgggcat gccgcttcct gttcctcagc tcgccgccgc
     3121 cgcccgagga gccgggtcag ccaggtcgtg cgccgtttct gcgcgagttg aaatctctcc
     3181 acgacagccg atcccgacat cagtgtcacc gatccgtcac cggcccgctg gacctgctgg
     3241 acggcctctc cgtaggcgcg gtgggccgct tcccggtcct cgccgcggtg gatgcagccg
     3301 gcgaaggagg tcagcactcg atagacgata gagttgtcca tatctctcgt tcgaacgtca
     3361 gcccccgcca ctttcggacg acccgcctca agggttttcg cggcagccgg cggggctggc
     3421 aaggggagga tgaagggggt tttactcacc gcgaaccgcg aaatggggat tccccacacc
     3481 gttgtgggtt gttttcggaa tctctctcct gccggtgtcg ccgtcttctc ttcttgtcct
     3541 ccagaacccc ctcagcggca acttagtgat ggacctgccg tgaaccggca caaaggagga
     3601 gcgcttggcg aaggacacga acgaggaggt cttggccacc gaacggaaga tctctctcct
     3661 ggggatcgcc ctggatcttc tgatgcggac gaacggctgg acggtgacgg ggctggcgaa
     3721 ggcggccggc gtctccccca agaccatctc ctcctatctc tgggaggagg ccctcacccg
     3781 ggagcgtttc gacgagctcg cccgcctcat ggggctgagc cgggtgaagg tcgagcgggc
     3841 ccaccgggcg gcagccctgg tggtgccccc gccaccagcc gcctggtcgc cggtggagcc
     3901 gacgccggag gagcgccgca tccatgagga ggccgcggtg ctggcaggct gcgaggtg
//
LOCUS       DASPJI010000712         6965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_102490, whole genome shotgun sequence.
ACCESSION   DASPJI010000712 DASPJI010000000
VERSION     DASPJI010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102490"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VEL74_20025"
     CDS             <1..502
                     /locus_tag="VEL74_20025"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HYG64880.1"
                     /translation="ALAGMGAGPIVIACVTVHHMLPGVPEPLRRKVVSLVELVIDEIL
                     RAPGPYLLLCTTGTRRARIFDGHPRWGEVAESVIFLDEADQHELHRQLYVLKDFGPPE
                     ECLRWLETLPAKYGRDRFIFACTELHLLHRALAGRPAQEKAGEEPFGILDPLWIVARD
                     ARRLLS"
     gene            complement(507..6956)
                     /locus_tag="VEL74_20030"
     CDS             complement(507..6956)
                     /locus_tag="VEL74_20030"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64881.1"
                     /translation="MINGYGPTEGTTFTCCHPMAHPDAVGTTVPIGTPIGNTRVLVLG
                     RDLQPVPAGVYGELHAGGDGLARGYLDRPDLTAERFVPDPFAGEPGARLYRTGDLVRW
                     RADGRLEFLGRRDGQIKLRGYRIELGEIESALARHPEVREAAVVVRDEVDGSGSKRLV
                     ACVVAQPAEQVDDEDAAGHVAQWQELYEETYSQGPRSGEPGEAGDATFNIQGWNSSYT
                     GEPIPAGEMREWLDGTVERLLALPHARVLEIGCGTGLLLFQVAPHAERYRGTDFSAAA
                     LAYVRRQLRNLPQVELAQGLADDWSGVAPGEADLVILNSVVQYFPGIDYLARVLEGAV
                     RAVAPGGAVFVGDVRSLPLLEAIHTSVELYQQDGSTTLPDLRRRIGRRVADEEELVIH
                     PAFFHALARRLPGISGVEVLVKLGRAHNELTRFRYDVVLYVGGRAEAPAGPVREAGSL
                     PEVEAFVASRPETLVLAGIPNARLAEEERALELLGTPERGVQTVEKLRRTVAGRLERE
                     GRGVDPQELWELGERLGYRVRLLWRLGSPFRFDAVLERRELPLSRARRAPAVTLESLD
                     IALDLPWTAYANDPLRRQLARRLVPELRRHLQDELPDYMVPAAFVLLDALPLTANGKV
                     DRRTLAGIEPVSAAAPVQGRVAPRTPAEERVAAVWRDVLGLESLGIHDDFFELGGHSL
                     LATQVISRLRQAAGVEIPLRTLFEAPTVAALAGEVETLTRAGGGLVAPPIVRTGRTER
                     DSDPPLSFAQEWMWLVDQLDPDGTTVISMIPVRLRGRLDVPALGAALDGLVRRHEVLR
                     TTFPLVDGRPVQRIAPAAGLRLPMVDLQGLPEAAREAEADALTLAESTGRMDLARGPL
                     LRALLLRLGPEDHSLLLAPHHILLDGWSLGVLLRETAALYQGVTLPELPVQYADYAVW
                     QRRWLQGEPLEALLGYWRRQLAAPLAVLELPGSRPDAAAMRSARVPVALGPELSAALR
                     ALAQSHGVTLFVALLAAFQALLHRYTRQTDIPVGSSVANRNSPEIEGLIGYFVNTLVL
                     RTGLGGDPGFSELLTRVREVALGAYAHQDLPFTRLVEELQPDRAASRTPLFQAFFLLQ
                     NMPMPKIELPGLAMTGLEPDAPTATFDLALVLSEGDQGLAGSLWYNRDLLEEETMTLL
                     MERFRTLLGGAAADPACRISALPLVSEAERRQLLAPSSSAPLSASAAPSGSAGPADNE
                     AGCLHRRFADRVQDDPEAVALVFEGDETTYRELEEASNRLAWHLRELGVGPDVLVGIC
                     LERSPEMVTALLAVLKAGGAYLPLDPAYPQERLRFLLRDAGTRVLVTRGGLAARLADL
                     PADLPQDLAIVDLDAEAPIIARRAIEAPVSGVGPENLAYVIYTSGSTGTPKGVMVTHA
                     NVARLFASTESWFRFDERDVWTLFHSYAFDFSVWEIWGALLHGGKLVIVPFMVSRSPE
                     AFHELLERERVTVLNQTPSAFAQLAFQLAREGADSRRALKSLRWVVFGGEALNLRNLE
                     VWFQNFGDRRPRLVNMYGITETTVHVTHRPISAADVTGGSMIGEPIPDLAVHLLDPHL
                     QPAVAGLPGEMYIGGAGLSRGYLGRPDLTAERFVPDPFSAVPGARLYRSGDLARQAPG
                     QSGHDLEYLGRADDQVKIRGFRIEPGEVEAALASHPAVAEAVVVAREDETGDKRLVGY
                     VVPAEPRALPARRLLRYQREGKLDGRATHELPNGMVVVHQHEGVTASIYKEIFEGEVY
                     RRHGIELPPGACIFDIGANLGLFTLWAGTTVPGARLFSFEPIPATADVLRLNAELYGL
                     NATVYAAGVADAERTEVFTFYPYFPSTSGRFPDLAKDRADIKAHILNEQRVLGEELGG
                     GGFEAWRHEREGMLDSWLDEHMKSERVTCRLTTVSSVLREHGLDRIDLLKIDAERSEL
                     DVLAGIAEEDWPKVRQMVIEVHDAAIRDRVLEILQARGFEAVVEQDVFLEGTELFNVY
                     ARRPEAPAARSESGDAPSETVWASPSRLSEEVRHWAVGRLPEHMVPSAVVVLESWPLT
                     AHGKLDRRALPEPAKAVKAVARPIVEPRTPVERELVEIWKELLRVDRVSIYDNFFELG
                     GHSLLLTQLVSRIRTAFQVEVPLRVLFDVKDVVEMTAAIAERQVAEVGEEDMAAMLDE
                     LKGLSPEEIKALLEAES"
BASE COUNT          970 a         2464 c         2643 g          888 t
ORIGIN      
        1 ggccctggcc ggcatggggg ccggccccat cgtcatcgcc tgcgtcaccg tccatcacat
       61 gctgcccggg gtgccggagc cgttgcggcg gaaggtggtg tcgctggtcg agctggtgat
      121 cgacgagatc ctgcgcgccc ccgggcccta cctcctgctc tgcaccacgg ggacgcgccg
      181 ggcacggatc ttcgacggcc atccgcgctg gggcgaggtg gcggagtcgg tgatcttcct
      241 cgacgaggcg gaccagcacg agctgcaccg ccagctctac gtcctcaagg acttcggccc
      301 ccccgaggag tgcctgcgct ggctcgagac cctgccggcc aagtacggcc gggaccggtt
      361 catcttcgcc tgcaccgagc tgcacctgct gcaccgcgcg ctggccggca ggcccgccca
      421 ggagaaagcg ggcgaggagc ccttcggcat cctcgacccc ctgtggatcg tggcccgcga
      481 cgcgcgccgc ctgctcagct agctgctcag ctctccgcct ccagcaacgc cttgatctcc
      541 tcgggggaga gccccttgag ctcgtcgagc atcgcggcca tgtcctcctc gccgacctcg
      601 gccacctggc gctcggcgat ggcggcggtc atctccacca cgtccttgac gtcgaagagg
      661 acgcgcagcg gcacctccac ctggaaggcg gtgcggatgc gcgacacgag ctgggtgagc
      721 agcagcgagt ggccgcccag ctcgaagaag ttgtcgtaga tgctcacccg gtcgacccgg
      781 agcagctcct tccagatctc gaccagctcc cgctccaccg gcgtccgcgg ctcgacgatg
      841 ggccgcgcca ccgccttgac cgccttggcc ggctcgggga gggcgcggcg gtcgagcttt
      901 ccgtgggccg tgagcggcca ggactcgagc accaccacgg ccgagggcac catgtgctcc
      961 ggcagccggc cgaccgccca gtggcgcacc tcctcggaga ggcgcgacgg gctcgcccac
     1021 accgtctcgg agggggcgtc cccgctctcg ctccgcgccg ccggggcttc cggccgccgc
     1081 gcgtagacgt tgaacagctc ggtcccctcg aggaacacgt cctgctccac cacggcctcg
     1141 aagccgcgcg cctggaggat ctcgaggacg cggtcgcgga tggcggcgtc gtgcacctcg
     1201 atcaccatct gccggacctt cggccagtcc tcctcggcga tgccggcgag cacgtcgagc
     1261 tcgctgcgct cggcatcgat cttgagcagg tcgatgcggt cgaggccgtg ctcgcggagg
     1321 accgatgaga ccgtggtcag gcggcaggtc acccgctcgc tcttcatgtg ctcgtcgagc
     1381 caggagtcga gcatcccctc gcgctcgtgg cgccaggcct cgaagccgcc gccccccagc
     1441 tcctcgccga gcacccgctg ctcgttgagg atgtgggcct tgatgtccgc ccggtccttg
     1501 gcgaggtcgg ggaagcggcc cgaggtcgag gggaagtagg gatagaaggt gaagacctcg
     1561 gtgcgctcgg cgtcggcgac gccggcggcg tagaccgtgg cgttcaggcc gtacagctcg
     1621 gcgttgaggc gcagcacgtc ggccgtggcc gggatcggct cgaaggagaa gaggcgggcg
     1681 ccggggacgg tggtccccgc ccacagggtg aagaggccca ggttggcgcc gatgtcgaag
     1741 atgcaggcgc ccgggggcag ctcgatgccg tgccgccggt agacctcgcc ctcgaagatc
     1801 tccttgtaga tcgacgccgt caccccctcg tgctggtgga cgaccaccat gccgttgggc
     1861 agctcgtggg tggcgcggcc gtccagcttc ccctcgcgct ggtagcgcag gagccggcgc
     1921 gccggcagcg cgcggggctc ggcggggacg acgtagccga cgagccgctt gtcgccggtc
     1981 tcgtcctcgc gcgcgaccac caccgcctcg gccaccgccg gatggctcgc cagcgccgcc
     2041 tccacctcac cgggctcgat gcggaagccg cggatcttca cctggtcgtc ggcccggccc
     2101 aggtactcga ggtcgtggcc cgactggccg ggtgcctggc gcgccaggtc gccgctgcgg
     2161 tagagccggg cgccggggac ggcggagaac ggatcgggga cgaagcgctc ggcggtgagg
     2221 tccgggcggc cgaggtagcc gcgggagagg ccggcgccgc cgatgtacat ctcgccgggg
     2281 aggccggcga cggccggctg caggtgcggg tccaggaggt ggaccgcgag gtccgggatc
     2341 ggctcgccga tcatgctgcc gccggttacg tcggcggcgg agatcggccg gtgggtcacg
     2401 tgcacggtgg tctcggtgat gccgtacatg ttgaccagcc gcggccggcg gtcgccgaag
     2461 ttctggaacc agacctcgag gttgcgcagg ttgagcgcct cgccgccgaa gaccacccag
     2521 cgcagcgact tgagggcgcg ccggctgtcg gcaccctcgc gggcgagctg gaaggcgagc
     2581 tgcgcgaagg cggagggggt ctgattgagc accgtcaccc gctcccgctc cagcagctcg
     2641 tggaaggcct cgggcgagcg gctcaccatg aacggcacga tgaccagctt gccgccatgg
     2701 aggagcgccc cccagatctc ccagaccgag aagtcgaacg cgtaggagtg gaagagggtc
     2761 cagacgtcgc gctcgtcgaa gcggaaccag gactcggtgg acgcgaacag gcgcgccacg
     2821 ttggcgtggg tgaccatcac ccccttgggc gtgccggtgg agcccgaggt atagatgacg
     2881 taggcgaggt tctcgggccc gacgccgctc accggcgcct cgatggcgcg gcgggcgatg
     2941 atgggggcct cggcgtcgag gtcgacgatc gcgaggtcct ggggcagatc ggccggcagg
     3001 tccgccagcc gcgcggcgag gccgccgcgg gtgacgagca cccgcgtacc ggcgtcccgc
     3061 aggaggaagc ggaggcgctc ctgggggtag gccgggtcga gcggcaggta ggcgccgccg
     3121 gccttgagca cggcgaggag ggcggtcacc atctcgggcg agcgctcgag gcagatcccc
     3181 accaggacgt ccggccccac gccgagctcg cgcaggtgcc aggccaggcg gttggaggcc
     3241 tcctccagct cgcggtaggt ggtctcgtcc ccttcgaaga ccagggccac ggcctcgggg
     3301 tcgtcctgga cgcggtcggc gaagcggcga tgcaggcagc cggcctcgtt gtccgccggg
     3361 cctgcgctcc cggacggagc ggcggaggcg gagaggggcg cggacgacga cggggccagg
     3421 agctggcggc gctcggcctc ggagaccagg ggcagggccg agatccggca ggccggatcg
     3481 gccgccgcgc cgccgaggag ggtgcggaaa cgctccatca gcagggtcat ggtctcctcc
     3541 tccaggaggt cgcggttgta ccagagggag ccggcgagtc cctggtcacc ctcggagagg
     3601 accagcgcca ggtcgaaggt ggcggtggga gcgtcgggct cgagccccgt catggcgagg
     3661 ccgggaagtt cgatcttggg catgggcatg ttctgcaaaa ggaagaaagc ctggaagagt
     3721 ggcgtccggc tcgcggcgcg gtccggctgc agctcctcca ccagacgggt gaacggaagg
     3781 tcctgatggg cataggcccc gagggccacc tcgcggacgc gggtcagcag ctcggaaaag
     3841 ccgggatcgc cgcccagacc ggtccgcaag accagggtgt tgacgaagta gccgatgagc
     3901 ccctcgatct cggggctgtt gcggttggcc accgacgacc ccaccgggat gtcggtctgc
     3961 cgggtatagc ggtgcagcag cgcctggaag gcggcgagga gcgccacgaa cagggtgacg
     4021 ccgtggctct gggcgagggc gcgcagggcg gcggagagct ccggccccag cgccaccggc
     4081 acgcgggcgc tgcgcatggc cgcggcgtcc ggccgcgagc ccggcagctc cagcaccgcc
     4141 agcggcgcgg cgagctgccg gcgccagtag ccgaggagcg cctccagggg ctccccctgg
     4201 agccaccggc gctgccagac ggcgtagtcg gcgtactgca ccggcagctc cggcagggtg
     4261 acgccctggt agagcgccgc cgtctcgcgc agcaacacgc cgagggacca gccgtcgagc
     4321 aggatatggt gcggcgcgag gagcagactg tggtcctcgg ggcccaggcg gagcagcagg
     4381 gcgcgcagca gcggcccgcg cgccaggtcc atccggccgg tgctctcggc gagggtgagg
     4441 gcgtcggcct cggcctcccg cgccgcctcg gggagcccct ggaggtcgac catgggcagg
     4501 cggaggcccg ccgcgggcgc gatccgctgc accggccgac cgtccaccag cgggaaggtg
     4561 gtgcgcagca cctcgtggcg gcgcaccagc ccgtcgagcg cggcgccgag cgcgggaacg
     4621 tcgagacggc cgcgcagccg caccgggatc atggagatga ccgtggtgcc gtcggggtcg
     4681 agctggtcca ccagccacat ccactcctgg gcgaacgaca gcggcgggtc gctgtcgcgc
     4741 tcggtccgcc cggtccgaac gatgggcggc gccacgaggc cgccgccggc gcgggtcagc
     4801 gtctcgacct cgccggccag ggcggcgacg gtgggcgcct cgaagagggt tcgcagcggg
     4861 atctccaccc ccgccgcctg gcgcaggcgc gagatcacct gcgtggcgag gagggagtgg
     4921 ccgccgagct cgaagaagtc gtcgtggatg cccaagctct ccaggccgag cacgtcgcgc
     4981 cagaccgccg cgaccctctc ctcggccggc gtgcgcggcg ccacccgccc ctgcaccggc
     5041 gcggcggcgg agaccggctc gatgccggcg agggtgcggc ggtccacctt gccgttggcc
     5101 gtgagcggca gggcgtcgag cagcacgaag gcggccggca ccatgtagtc cggcagctcg
     5161 tcctggaggt ggcgccgcag ctccggcacc aggcgccggg cgagctggcg gcgcagcggg
     5221 tcgttggcgt aggccgtcca ggggaggtcg agggcgatgt ccaggctctc cagggtgacg
     5281 gccggcgcgc ggcgagcccg ggagagcggc agctcccggc gctcgaggac ggcgtcgaag
     5341 cggaagggcg agcccagccg ccagaggagg cgcacgcggt agccgagcct ctcgcccagc
     5401 tcccagagct cctggggatc gacgcctcgg ccctcccgct ccagccggcc ggcaacggtg
     5461 cggcgcagct tctccaccgt ctggacgccc cgctcggggg taccgagcag ctcgagggcc
     5521 ctctcctcct ccgccagccg ggcgttgggg atgccggcga ggaccagcgt ctcgggccgg
     5581 gaggccacga aggcctcgac ctccggcagg gatccagcct cacggaccgg cccggcaggc
     5641 gcctccgccc ggccccccac gtacagcacc acgtcgtacc ggaagcgggt cagctcgttg
     5701 tgggcccggc cgagcttgac cagcacctcg acgccggaga tcccgggcag gcgccgtgcc
     5761 agggcatgga agaaggccgg atggatcacc agctcctcct cgtccgccac ccgccggccg
     5821 atccgccgcc gcaggtccgg cagggtcgtg gagccgtcct gctggtagag ctccaccgag
     5881 gtgtggatcg cctcgaggag cggcaggctg cggacgtcgc ccacgaacac cgcccctccc
     5941 ggagcgacgg cgcgcaccgc cccttccagc acccgggcca ggtagtcgat gcccggaaag
     6001 tactgcacca ccgagttgag gatcaccagg tcggcctcgc cgggcgcgac gccgctccag
     6061 tcgtcggcga ggccctgggc gagctcgacc tgcggcaggt ttcggagctg gcggcggacg
     6121 taggccagag ccgcggccga gaagtcggtg ccgcggtagc gctcggcgtg gggggccacc
     6181 tggaagagga gcaggcccgt gccgcagccg atctcgagca cccgggcgtg cggcagggcg
     6241 agcagccgct ccacggtgcc gtcgagccac tcgcgcatct cgcccgccgg gatcggctcg
     6301 ccggtgtagc tgctgttcca gccctggatg ttgaaggtgg cgtcgccggc ctccccgggc
     6361 tcaccggagc gggggccctg gctgtaggtc tcctcgtaga gctcctgcca ctgggccacg
     6421 tgcccggcgg cgtcctcgtc gtccacctgc tccgccggct gggccacgac gcaggcgacc
     6481 aggcgcttgc tccccgagcc gtcgacctcg tcgcgcacca cgaccgccgc ctcgcgcacc
     6541 tcggggtgcc gggctagggc gctctcgatc tcccccagct cgatgcggta gccgcgcagc
     6601 ttgatctggc cgtcgcgccg ccccaggaac tcgagccggc cgtcggcccg ccagcgcacc
     6661 aggtcgcccg tgcggtagag gcgggcaccg ggctcgccgg cgaagggatc gggcacgaag
     6721 cgctccgccg tcaggtcggg acggtcgagg tagccccggg cgaggccgtc gccgccggca
     6781 tggagctcgc cgtagacgcc ggccggcacc ggctggagat cgcggccgag caccaggacg
     6841 cgggtattgc cgatgggagt cccgatcggg acggtggtgc ctacggcgtc cggatgggcc
     6901 atcgggtggc agcaggtgaa ggtcgtcccc tcggtggggc cgtagccgtt gatcaccgtg
     6961 cagcc
//
LOCUS       DASPJI010000713        14712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_270242, whole genome shotgun sequence.
ACCESSION   DASPJI010000713 DASPJI010000000
VERSION     DASPJI010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14712)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14712
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270242"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VEL74_20035"
     CDS             complement(<1..476)
                     /locus_tag="VEL74_20035"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HYG64882.1"
                     /translation="MSLLRTAFTEQAGIEVPLICGAMYPCSNPELVAAASEAGGIGII
                     QPLSLVYVHRHPFRDGLRLIRGLTAKPVGMNVIVEKSSKVYLERMQGYVDTALEEGIR
                     FFVTSLGNPRWVVEKAHAVGGLVYHDVTDRRWAEKGLEAGVDGLIAVNDRAGGHAGG"
     gene            complement(528..1307)
                     /locus_tag="VEL74_20040"
     CDS             complement(528..1307)
                     /locus_tag="VEL74_20040"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HYG64883.1"
                     /translation="MRALLVAAHPDDETIGAAGLLSRLPRCELLYLTDGAPRDLRWAP
                     AARGSRDEYAEARRREAAAALSLVGLADHSLISLGAVDQEAAFAMPVLARHLADRFHR
                     FGADLVLTHAYEGGHPDHDAAAFIVHAAAELLRRRGASPPLLVEMTSYHARGGAFVAG
                     EFLPLGGLDGQALLNLTPKERGLKRRMLECFETQKEFLVQFPLPEVECYRPAPAYRFT
                     EPPHAGPLLYETRGFPLAGGHWRELARQALRDLDLPEDEPF"
     gene            complement(1447..13617)
                     /locus_tag="VEL74_20045"
     CDS             complement(1447..13617)
                     /locus_tag="VEL74_20045"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64884.1"
                     /translation="MISLEPTPLPSFRSLDSEEPGPARLHETFEEQADRTPEAPALVS
                     ADLSLTYRELEARANRLAHHLISLGVGPEVRVGVFLGRSAETVVSILAVLKAGGAYVP
                     LDPAYPAERVAFMLEDSRAAGVLTDESRRSALPPGPGWIVSLDADARALADRPASRPL
                     PGGLPDGLAYVIYTSGSTGRPKGVMIRHRSAAGLVAWARQVFTLEELEGVLAATSFCF
                     DLSVFELFVPLSRGGTVILARDALALPEAAWLRPVTLVNTVPSAMAELLRSGAVPPSV
                     RTVNLAGEALPGALVDGLYRAGVERVFNLYGPSEDTTYSTFALIPPGSAHPPIGRPVA
                     NTRAWVLVAGPEIDPRQVAAGEEGDLYLGGEGLARGYLDRPDLTAERFVPDPWAASPG
                     ERLYRTGDLVRRLPGGDLLYLGRIDQQVKIRGFRIELGEVEAALSRHPSVREAAVAAL
                     EGPSGERRLVGFLVAEPGLEIPPARELRRSLRRSLPEPMVPPAFTVVPALPRTVNGKL
                     DRAALTALQGESTGAGAAFVAPRTPVEERLAGFWRELLGVEDVGVHDDFFELGGHSLL
                     ATRLVSRLREGLGVELSGAEVLGHPTVASLAARLETLASGERGAASSSFAPIEPVDHE
                     GELPLSLMQEQFFFLDRLAPGLPAYDLPAAWRLAGALDEAALERAFGEVLRRHEALRA
                     VFGLRDDGPVQRVLPPAPFTLPRIDLRALPAVAREAESRRLLREEAGWRFDLLRGPLL
                     RAVLIHLEGDHPAVHALAVNIHHIAADGWSLSLLAHELRELYGAFRQGALPSLPALPV
                     QYGDFAVWQRRWLTQELLAPHLAYWRERLAGIVPLDLPADRPRPPVQTFRGRTLVLTV
                     PAALKEGVARLARQVGATPFMALLAGFQTLLHRLTGAADVVVGVPVALRGRREVEDLI
                     GLFVNTLVLRADFTQGEAMGFRDLLAQARQTALEAYAHQDLPGERLVEELQPVRDLSR
                     NPLVQQMFQLLDDAEPLALADVAVEPLPVDNGTAQFDLGLTLTEEAGGWTGRLESSSD
                     LFDETTVRRWAEHYLRLLAEAVAGPDRAVADLPLLAPAERHQVLVEWNDTGEALAPLT
                     VPERLAERAAIAPDAVAVRFADESLTYGELIRRARRLAARLTAQGAGPEGVVGLAVER
                     SPALVIGLLGIWEAGAAYLPLDPSHPAERLALLLEDAGTGLVVTEEPVRERLALPRER
                     AVLLEEETPLPDSPFPVPARSGGALAYVIYTSGSTGTPKGVRVEHGNLARILAACQAE
                     LGFHAGDVMAVLAPYSFDIFLFELLSPLLAGGTAVLVPSRPALDIRKVVSLLPELTRL
                     HAVPSLMRQIVAAARWLPPASRRPATLFVGGDAVPADLLSEMRQTFPEAGVRVLYGPT
                     EATILASSQRVGEASSLPQAPLGRPLPGVELRVADGRGRPQPIGVPGEIWIGGAGVTR
                     GYLGRDELTAERFPMVDGRRWYRTGDLGRWLAGGNLEFLGRVDGQVKVRGVRIEPGEI
                     EAALLAHPAVREAAVLAQEEPGGDRRLVAYLEAPAGETDAAGLRAFLQGRLPGAMIPS
                     LFVLLPTLPLTATGKVDRRALAALAPAAAPDLPVASFATPTEEVLAVIWADLLGLDRV
                     GAAGHFFYLGGHSLLAYRLLSRVRESFGVDLPIRTLFESPTVAGFAARLDEALRSGRA
                     RGLPPVTPVGARTGDLPLSFSQQRLFFLDRLMPGNPAYNLPVGFRLAGPVAPAALEAG
                     LGEIVRRHEVLRTAFALVKDGEEERAVQRVAPPASFLLPRVDLEGLPPGVRETELLRQ
                     AAAEARQPFDLLRGPVFRAVLLRLGEEDHALFLNFHHIATDAWSLEVMLRELSLLYGA
                     VVAGLPSPLPELPVQYADFAVWQRRWLAGEALEAHARWWKERLAGAPVVLELPADRPR
                     PAVQSHRGGAFTMPLPRGLGDGLARLARRHGATLYMTLLAGFQTLLARLSGQDDLLVG
                     SPVANRGRREVEGLIGFFVNTLVLRGGLAGDPGFLGALARVRETALGAYAHQELPFER
                     LVEELQPVRDTSRNPILQVLFQLLPAEAPPFALAGAETRLLSRGSGSVRFDLETAVVT
                     GGNGGLVADVEYARDLFDAPTVLRFFEQYGRLLEGVLADPERRLSEIPLLGEAGEHQV
                     LREWNDTAAPAPAARRLQEVFEAQAARTPAAVAVTSAGRSLTYCELEAGANRLARHLQ
                     ALGVGPETRVGIFLERSVDLVLAVLGVLKAGGTYLPLDPAYPRERLAFMLADAGAAGA
                     VTQESLAGALPPVPWVVRLDRDGEALSRWEATPPPCPAEPEGVAYLIYTSGSTGTPKG
                     VAIRHRAAAEMLAWAGGVFSPERAAATLASTSISFDLSVFEIFLPLCRGGRAVLVRDA
                     LALAGLQEEVTLVNTVPSAMTELVRTGALPASVTTIGLAGEALPGSLVDGIHRLPALP
                     ATGARQVFNLYGPSEDTTYSTFTRIDPGTPFPAIGRPIANTRGYVLDRALQPVSPGVA
                     GELFLAGEGLARGYLGRPGLTAERFVPDPWSAAPGGRLYRTGDLVRALPDGSLRLLGR
                     LDHQVKVRGFRIELGEVEAALLRHPAVREAVAAVREEAPGAKRLVAYLAASAELEPGE
                     VRDFLRRTLPEPVLPSAFVVLPELPHTPNGKVDRKALPAPGFESRGAGEPAAPRTPTE
                     EVVAGIWTAVLGIERLGVHDNFFDLGGHSLLAYRLLSRVRESCGVELPIRALFEAPTL
                     QGFATRVNEAARGQGLPPVTPASRPPVDGELPLSFSQQRLLFLDRLMPGNPVYNLPLG
                     FRIAGPLDVPALEKALGEIVRRHEVLRTSFGLADERAFQRVAPPAPLPLPRADLRGLP
                     PSVREAELLRQAAAEARQPFDLLAGPVFRALLLQMADGDHALLLNLHHIATDAWSLDV
                     MLRELSALYGAFARGLASPLPELPVQYADYAAWQRRWLAGEALEAQAGWWKERLAGAP
                     MVLELPPDRPRPAVQTHRGATLAIPLPAGLEEGLTRLGRGAGATLFMTLLAGFQGLLA
                     RLSGQDDVLVASPIANRGRREVEGLIGFFVNTLVLRARFADDPVFGALLARVREAALG
                     AYAHQDLPFERLVEELQPERDTSRNPIAQVMFQLLAEADTPLDLAGAEVRALPRDSGA
                     ARMDLELAVVRSDGALIAFAEYATDLFDAVTILRLVEQYGRLLEAVVADPGRRLSEIP
                     LLGEAGAHQLLHEWNDTARPAPAAGRLQEVFEAQAARTPTATALVAAGRSITYGELEA
                     AANRLARHLQSRGVGPEARVGIFLDRSVDLVVSVLGVLKAGGAYLPLDPAYPRERLAF
                     MLADAAAAGAVTRGDLAGSLPPVPWTVHLDRDQETLAAEDGIRPPCAAEPESLAYVIY
                     TSGSTGRPKGVAIRHRAAAEMVAWAGEVFSPAMTAAALASTSICFDLSVFELFLPLSQ
                     GGRVVLVRDALALASAETGAGEGVTLVNTVPSAMTRLVQAGAVPASVTTVGLAGEPLP
                     GALVDAIHGLPGGENRRVFNLYGPSEDTTYSTWALVRPGTRLPPIGRPVSNTRAYVVD
                     RAFQPVPPGVAGELCLGGEGLARGYLGRPELTAERFVPDPWSGTAAGAGSRLYRTGDL
                     VRTLPDGELRLLGRLDHQVKVRGFRIELGEVEAALLGHPAVLEAVVVAREDTPGEKRL
                     AAYLATAGTPVATLEDSELRELLRRTLPEYMVPSAFVVLPELPHTPNGKVDRKALPPP
                     DLESRPAGGEPREPVAPRTPTEAGLVKIWKKVLGIERLGVQDSFFDVGGHSLLALHVV
                     TRIRGQFGLDLPLAAMFRAPTIEAMAALIDGQAPAVTSPVILMGGAGIPKTPFFCVHG
                     VGGNVFRLRELSQLLGRPFYGVQGWSDLKDISYLGSVDAMVSRYLEEVRKVQPEGPYL
                     LGGICVGCHIAFEMARRLTADGRTVAHLVFLDTQAGGQADGALDFASLEAAVADELGI
                     PIARQKLLALPPEGRLEYLVREGRRSQALPPGFTTADARRYIDVFRLNLNAWNEHRPT
                     PWEVRATLFAGEGLEDDPSAGWGAYALGGCGFVEVTGDHATMLRSPHVEGLAAKLARV
                     LDGEEARLRG"
     gene            complement(13639..>14712)
                     /locus_tag="VEL74_20050"
     CDS             complement(13639..>14712)
                     /locus_tag="VEL74_20050"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HYG64885.1"
                     /translation="VPVLNVFFADAGGRIAWTILGRVPRRVGFDGVVAGSWADGSRRW
                     DGLLPPEEMPQIVDPGSGRLWNGNNRSASPEMMEKIGGRIHIHGARARQIRDGLFAVE
                     KATVEDMRRIQLDDRALFLQRWRDQLLGVLTPEAVAADPRRGEMREAVENWGGRAAAE
                     SAGYRMVRGYRMYLSQSVFQPLLTRCGGLPEDFPYLFAFHAAEEPLWAVVTQQPAHLL
                     NPEYKSWQEQFLAAVDTMLAQLPPGPVQERTWGEFNTVRIQHPVSQAVPFLGRWLDMP
                     RRPMSGDAEMPRVMTVRFGPTLRMVVSPGREQEGYFHMPGGQSGHPLSEHYADGFDAW
                     AEGKPTPFLPGPAVKTLRLVPGT"
BASE COUNT         2178 a         5415 c         5410 g         1709 t
ORIGIN      
        1 ccgccggcgt gcccgccggc cctgtcgttg accgcgatca ggccgtccac ccccgcctcc
       61 agccccttct cggcccagcg ccggtcggtg acgtcgtgat agacgagccc gccgaccgcg
      121 tgcgcctttt ccactaccca gcgcgggttg ccgagggagg tcacgaagaa gcggattccc
      181 tcctcgagcg cggtgtccac gtacccctgc atgcgctcga gatagacctt ggaggacttc
      241 tcgacgatga cgttcatccc caccggtttc gcggtgaggc cgcggatgag ccgcaggccg
      301 tcgcggaacg ggtgccgatg gacatagacc agcgacagcg gctggatgat gccgatgccg
      361 ccggcctcgg aggcggcggc caccagctcg gggttgctgc acgggtacat ggcgccgcag
      421 atcagcggca cctcgatgcc ggcctgctcg gtgaacgcgg tcctcagcag ggacatggcc
      481 tgacctcctc cggaacggga ggattgtatc ccgccggggg gtgaggccta gaagggctcg
      541 tcctccggca ggtccagatc ccggagggcc tgccgggcca gctcgcgcca gtgcccgccg
      601 gcgagcggga agccccgcgt ctcgtacagc agagggcccg cgtggggagg ctcggtgaaa
      661 cgataggccg gggccggccg gtagcattcg acctccggca gggggaactg cacgaggaac
      721 tccttctgcg tctcgaagca ctcgagcatc cgccgcttga gcccccgctc cttgggagtc
      781 agattgagca gcgcctgccc gtccagtccg ccgagcggca ggaactcgcc ggccacaaac
      841 gcgccgccgc gtgcatggta ggaggtcatc tccaccagga gcggcggcga ggcgccccgg
      901 cggcgcagga gctcggccgc ggcgtggacg atgaaggcgg cggcgtcgtg atccggatgg
      961 cccccttcgt aggcgtgggt gagcacgagg tcggcgccga agcggtggaa gcggtcggcg
     1021 agatggcggg cgaggaccgg catggcgaag gccgcctcct ggtccaccgc tcccagggag
     1081 atcaggctgt ggtccgccag gccgacgagg ctcagcgccg ccgccgcctc gcggcgccgc
     1141 gcctcggcgt actcgtcgcg cgagccgcgg gccgccggtg cccagcggag gtcccgcggc
     1201 gcgccgtcgg tgaggtacag cagctcgcac ctcggcagac gggaaagcag gccggcggca
     1261 ccgatcgtct cgtcgtccgg atgggccgcg accaggaggg cacgcaaagt gggaacccgt
     1321 ccgtcacctt gcgcaatcag attccgaaca atgcgccaag tggcgcttct gtcacctttt
     1381 gactggtcgt gagcagcgtc tcgcgacatg tcgaggcgca tggtgcaagg ggtgtgccga
     1441 gtcgcttcag ccccgcagcc gcgcctcctc gccgtccagc accctcgcca gcttcgccgc
     1501 cagcccttcg acgtgagggc tgcgcagcat ggtggcgtga tccccggtga cctcgacgaa
     1561 gccgcacccc ccgagggcgt aggcgcccca gccggcggac gggtcgtctt cgagcccctc
     1621 gccggcgaac agggtggcgc gcacctccca gggggtggga cggtgctcgt tccaggcgtt
     1681 caggttgagc ctgaaaacgt cgatgtagcg gcgggcgtcc gcggtggtga agcccggcgg
     1741 cagggcctgg gagcgccgcc cctcccgtac caggtactcc agcctcccct cggggggcag
     1801 ggcgaggagc ttctggcggg cgatggggat ccccagctcg tcggccaccg cggcctcgag
     1861 gctggcaaag tcgagcgcgc cgtcggcctg gccgcccgcc tgggtatcca ggaagacgag
     1921 gtgggccacg gtccgcccgt ccgcggtgag ccgccgcgcc atctcgaaag cgatgtggca
     1981 gccgacgcag atgcctccca ggagataggg tccctcgggc tgcaccttgc ggacctcctc
     2041 caggtagcgg ctcaccatgg cgtcgacgct gccgaggtag gagatgtcct tgaggtcgga
     2101 ccacccctgg acgccataga acggccgccc gaggagctgc gagagctccc gcagccggaa
     2161 gacgttgcca cccaccccgt ggacacagaa gaacggggtc ttcgggatgc cggcgccgcc
     2221 catgaggatc accggcgagg tcaccgccgg agcctggccg tcgatcaggg ccgccatggc
     2281 ctcgatggtc ggcgcccgga acatggcggc gagcggcagg tcgagcccga actggccgcg
     2341 gatgcgcgtc accacgtgca gggcgagaag ggagtggccg ccgacgtcga agaagctgtc
     2401 ctggacgccg aggcgctcga tccccagcac cttcttccag atcttgacca gccccgcctc
     2461 ggtgggggtc cgcggcgcca ccggctccct gggctcgcca ccggcaggac ggctctccag
     2521 gtccgggggc ggcagggcct tgcggtccac cttgccgttg ggcgtgtgcg gcagctccgg
     2581 caggacgacg aaggcggacg gcaccatgta ctcgggcagg gtgcggcgca ggagctcccg
     2641 cagctcgctg tcttccaggg tcgcgaccgg cgtgccggcg gtggcgaggt aggcggccag
     2701 ccgtttctca cccggcgtat cctcccgcgc caccaccacg gcctccagca ccgcgggatg
     2761 gccgagcagg gccgcctcga cctcgcccag ctcgatccgg aagccgcgca ccttgacctg
     2821 gtggtcgagc cggccgagca ggcgcagctc gccgtccggc agggtgcgga cgaggtcgcc
     2881 ggtgcggtag agccggctgc cggcaccggc ggccgtgccg ctccagggat cggggacgaa
     2941 ccgctccgcc gtcagctccg gccggccgag atagccgcgg gccagcccct cgccgccgag
     3001 acacagctcg cccgccaccc ccgggggcac cggctggaag gcgcggtcca ccacataggc
     3061 gcgggtgttg gagaccggcc gcccgatcgg cggcagccgg gtacccgggc ggacgagggc
     3121 ccaggtggag taggtcgtgt cctcggacgg gccatagaga ttgaagaccc gccggttttc
     3181 gccacccggg aggccgtgga tggcgtccac cagcgcgccg gggagcggct cgccggcgag
     3241 gccgaccgtg gtcaccgacg ccggcaccgc gccggcctgc accagccggg tcatcgccga
     3301 gggcaccgtg ttgaccagcg tcaccccctc ccctgcccct gtctccgccg acgccagcgc
     3361 cagcgcgtcg cgcaccagca ccacccggcc cccctgggac agggggagga acagctcgaa
     3421 gaccgaaagg tcgaagcaga tggaggtgga ggccagggcc gccgccgtca tggccgggga
     3481 gaagacctcg ccggcccagg ccaccatctc ggcggcggcg cggtggcgga tggccacgcc
     3541 cttgggacgg ccggtcgagc ccgaggtgta gatgacgtag gccaggctct ccggctcggc
     3601 ggcgcagggg ggccggattc catcctcggc ggccagtgtc tcttggtcgc gatccaggtg
     3661 gaccgtccag ggaacgggcg gcagggatcc cgcgagatct ccccgggtga cggcgccggc
     3721 cgcggcggcg tccgccagca tgaaggccag ccgctcccgg ggataggcgg ggtccagcgg
     3781 caggtaggca ccgccggcct tgagcacgcc gaggacggac accaccaggt ccacggagcg
     3841 gtcgaggaag atccccaccc gcgcctccgg ccccacccct cgggactgga ggtggcgcgc
     3901 cagccggttc gccgccgcct ccaactcgcc gtaggtgatg gacctgccgg cggcaaccag
     3961 cgccgtcgcc gtgggggtcc gcgccgcctg cgcctcgaag acctcctgca ggcgccccgc
     4021 cgcgggagcc ggccgggcgg tgtcgttcca ctcgtggagg agctgatgcg ccccggcctc
     4081 gcccagcagg gggatctccg acagccgccg ccccgggtcc gccacgaccg cctccagcag
     4141 gcgcccgtac tgctcgacca gccgcaggat ggtcacggcg tcaaacagat cggtggcgta
     4201 ctccgcgaag gcgatgagcg cgccgtccga acggacgacc gccagctcca ggtccatgcg
     4261 cgcggcgccg ctgtcgcgcg gcaaggcccg gacctcggcg ccggcgagat ccagcggcgt
     4321 gtccgcctcg gccaggagct ggaacatcac ctgggcgatg gggttgcggc tcgtgtcgcg
     4381 ctcgggctgc agctcctcga cgagacgctc gaacggcagg tcctggtggg cataggcccc
     4441 cagcgccgcc tcgcgcaccc gggcgaggag ggcgccgaag accgggtcgt cggcgaagcg
     4501 ggcgcggagc accagggtgt tgacgaagaa cccgatcagc ccttcgacct cccgccggcc
     4561 gcggttggcg atcgggctcg ccaccagcac gtcgtcctgc ccggagagac gcgccagcaa
     4621 tccctggaaa ccggccagga gggtcatgaa cagggtcgct ccggcacccc ggccgaggcg
     4681 ggtcagcccc tcctccagtc cggcgggcag tgggatggcg agggtggcgc cccggtgcgt
     4741 ctgcaccgcc ggacgcggcc ggtccggggg cagctccagg accatgggag cgccggcgag
     4801 cctctccttc caccaccccg cctgggcttc cagcgcctcc ccggcgagcc accggcgctg
     4861 ccaggcggcg tagtcggcgt actggaccgg cagctccggc agaggcgagg cgaggccgcg
     4921 ggcgaaggcg ccgtacaggg cggacagctc gcgcagcatg acgtcgagcg accaagcgtc
     4981 ggtggcgatg tggtggaggt tgaggagcag cgcgtggtcc ccgtcggcca tctggagcag
     5041 gagggcccga aataccggcc cggccagcag gtcgaacggc tggcgtgcct cggcggcggc
     5101 ctgccggagc agctcggcct cgcggacgga cggcggcagg cctcggaggt ccgcccgcgg
     5161 cagtggcagg ggggccggcg gcgccacgcg ctggaaggcc ctctcgtccg cgagcccgaa
     5221 gctcgtccgc aggacctcgt gacggcggac gatctccccc agggctttct cgagcgccgg
     5281 cacgtccagc ggcccggcga tccggaagcc caggggcagg ttgtagaccg ggttgccggg
     5341 catgagccgg tcgaggaaga gcaggcgctg ctgggaaaac gacaggggca gctcgccatc
     5401 aaccgggggc cgggatgccg gcgtgaccgg cggcagcccc tgccccctgg cggcctcgtt
     5461 tacccgggtc gcgaacccct gcagggtcgg cgcctcgaac agggcgcgga tgggcagctc
     5521 cacgccgcag ctctcgcgga cacgggagag caggcgatac gccagcagcg agtggccgcc
     5581 caggtcgaag aagttgtcgt ggaccccgag gcgctcgatc cccagcaccg ccgtccagat
     5641 cccggccacc acctcctcgg tgggggtgcg cggcgccgcc ggctcccccg caccgcggct
     5701 ctcgaaaccg ggcgcgggca gggccttgcg gtccaccttg ccgttcggcg tgtggggcag
     5761 ctcgggcagc accacgaagg cggacggcag cacgggctcc ggcagggtgc gccgcaggaa
     5821 atcccggacc tccccgggct ccagctccgc gctcgcggcc aggtaggcca ccagccgctt
     5881 cgcccccggc gcctcctccc gcacggccgc cacggcctcg cgtacggccg gatggcggag
     5941 cagcgccgcc tctacctccc ccagctcgat ccggaagccg cgcaccttga cctgatggtc
     6001 gaggcggccc agcaggcgca ggctgccgtc cggcagggcc cggaccaggt cccccgtccg
     6061 gtagaggcgc ccgcccggcg cggcgctcca ggggtccggc acgaaccgct ccgccgtcag
     6121 gccgggccgg ccgagatagc cgcgggcgag cccctccccg gccaggaaca gctcgccggc
     6181 gacgccggga ctcaccggct gcagggcgcg gtccagcacg tacccccggg tgttggcgat
     6241 cggccggccg atggccggga acggcgttcc gggatcgatc cgggtgaagg tcgagtaggt
     6301 ggtgtcctcg gaggggccat agagattgaa gacctgccgc gcccccgtgg ccggcagagc
     6361 cggcaggcgg tgaatgccgt ccaccaggct gccgggcagc gcctcgccgg cgagcccgat
     6421 cgtggtcacc gaggccggca gcgcgcccgt ccgcaccagc tccgtcatcg ccgacggcac
     6481 cgtgttgacc agcgtcacct cctcttgcag gccggccagc gccagggcgt cgcgcaccag
     6541 gacggcccgg cccccccggc acagggggag gaagatctcg aagaccgaca ggtcgaagct
     6601 gatggaggtg gaggccaggg tcgcggcggc cctctccggg gagaagacgc cgccggccca
     6661 cgccaacatc tcggcggcgg cgcggtggcg gatggccacc cccttgggcg tccccgtgga
     6721 gcccgaggtg tagatcaggt aggcgacgcc ctccggctcc gccgggcacg gcggcggggt
     6781 cgcctcccac cggctcaggg cttcgccgtc ccggtccaga cggaccaccc aggggaccgg
     6841 aggcagcgcc ccggcgaggc tctcctgcgt gaccgctccg gccgcgccgg cgtcggccag
     6901 catgaaggcg aggcgctccc gcggataggc cggatcgagc ggcaggtagg tgccgccggc
     6961 cttgagcacg cccaggaccg ccagcaccag gtccaccgag cgctcgagga agatccccac
     7021 ccgcgtctcg ggcccgacgc ccagggcctg gaggtggcgc gccagccggt tcgcccccgc
     7081 ttcgagctcg cagtaggtca gggaccggcc ggcggaggtg acggccaccg ctgccggcgt
     7141 cctcgccgcc tgggcttcga acacctcctg gagccgccgg gccgcggggg ccggcgccgc
     7201 cgtgtcgttc cactcgcgga ggacctggtg ctccccggct tcgccgagca gggggatctc
     7261 ggagaggcgc cgttcgggat cggccagcac cccttccaac aggcggccgt actgctcgaa
     7321 gaagcgcagg acggtgggtg cgtcgaacag gtcgcgggca tactcgacgt cggccacgag
     7381 gccgccgttg ccgccggtca cgaccgcggt ctccaggtcg aagcggacgg agccgctgcc
     7441 gcgcgacagc agccgcgtct cggcgccggc gagagcgaac ggcggcgcct cggccggcag
     7501 gagctggaag agcacctgga ggatcgggtt gcggctggtg tcgcgcaccg gctgcagctc
     7561 ctcgacgagg cgctcgaacg gcagctcctg gtgggcgtag gcgcccagcg ccgtctcccg
     7621 tacccgggcc agggcgccga ggaagcccgg atcgcccgcc aaaccgcccc gcagcaccag
     7681 cgtgttgacg aagaacccga tcagaccctc gacctcgcgg cggccgcggt tggccaccgg
     7741 gctgcccacc agcaagtcgt cctgtccgga gagacgggcg agcagggtct ggaagccggc
     7801 gaggagcgtc atgtagaggg tcgccccatg acgccgcgcc aggcgcgcca gcccgtcgcc
     7861 cagaccgcgc ggcagcggca tggtgaacgc gccgccgcgg tggctctgca cggccggacg
     7921 cggccggtcc gccggcagct ccagcaccac gggagcgccg gcgagcctct ccttccacca
     7981 gcgggcatgg gcctccagag cctcgcccgc gagccaccgg cgctgccaga ccgcgaagtc
     8041 cgcgtactgg accggcagct ccggcagagg cgaagggagg ccggccacga cagcgccata
     8101 gaggagcgac agctcgcgca gcatcacctc cagagaccag gcatcggtgg cgatgtgatg
     8161 gaagttgagg aacagggcat ggtcctcctc gcccagccgc agcaggaccg ccctgaacac
     8221 cggcccgcgc agcaggtcga acggctggcg cgcctcggcc gcggcctggc ggagcagctc
     8281 agtctcgcgg acgcccggcg gcagaccctc caggtccacc cgcggcagaa ggaacgaggc
     8341 aggcggcgcc acgcgctgga cggcccgctc ctcctctcca tccttcacca gggcgaaggc
     8401 ggtgcgcagg acctcgtgcc ggcggacgat ctcccccagg cccgcctcca gggcagccgg
     8461 agcgacgggg ccggcgagcc ggaacccgac gggcaggttg taggccggat tccccggcat
     8521 gagccggtcg aggaagaaca gccgctgctg ggagaacgac agagggaggt cgcccgtccg
     8581 cgctcccacc ggagtcaccg gcggcaggcc gcgggccctg ccggagcgca gggcctcgtc
     8641 gaggcgggcg gcgaagccgg ccacggtcgg gctctcgaac agggtccgga tgggcaggtc
     8701 gacgccgaag ctctcgcgca ccctggagag gagccggtag gccagcagcg agtggccgcc
     8761 gagatagaag aagtggcccg cggcgccgac ccgatcgagg ccgaggaggt cggcccagat
     8821 cacggccagg acctcctccg tcggcgtggc gaaggacgca acgggaaggt ccggcgcagc
     8881 ggcgggagcg agggccgcca gggcgcggcg gtccaccttg ccggtggcgg tgaggggcag
     8941 agtcgggagg aggacgaaga gcgaagggat catcgcgccc gggagccgcc cctggaggaa
     9001 ggcccggagc ccggcggcgt ccgtctcccc tgctggtgct tccagatagg cgacgagccg
     9061 gcggtcgccg cccggctcct cctgcgccag gacggccgcc tcgcgcaccg cggggtgggc
     9121 gaggagcgcg gcctcgatct cccccggctc gatgcgcaca ccacgcacct tcacctggcc
     9181 gtcgacccgg cccaggaact ccaggttgcc tccggcgagc cagcgcccca ggtcgccggt
     9241 gcgataccag cggcggccgt ccaccatggg gaagcgctcg gcggtcagct cgtcccgccc
     9301 gagatagcca cgggtcacgc cggcgccgcc gatccagatc tcccccggca cgccgatcgg
     9361 ctggggacgg ccccggccgt ccgccacccg cagctccacg ccgggcagcg gccggcccag
     9421 gggggcctgg ggcagggacg aggcctcccc caccctctgg ctggaggcca ggatggtcgc
     9481 ctccgtgggt ccgtagagca cccggacgcc ggcttccgga aaggtctgcc gcatctctga
     9541 caggaggtcc gccgggaccg cgtcgccgcc cacgaagagg gtggccgggc ggcggctcgc
     9601 cggcggcagc cagcgcgccg cggccacgat ctggcgcatc agggacggca ccgcgtgcag
     9661 gcgggtcagc tccggcagga gggagacgac cttccggatg tcgagggcgg gccgggaggg
     9721 caccaggacc gcggtccccc ccgccagcag cggcgacagc agctcgaaca ggaagatgtc
     9781 gaaggagtag ggagcgagca ccgccatgac gtcgccggca tggaagccca gctccgcctg
     9841 gcacgccgcc aggatacggg ccaggttgcc gtgctccacc cgcaccccct tcggcgtccc
     9901 cgtcgacccc gaggtgtaga tcacataggc cagggccccg ccggaccggg cgggcacggg
     9961 gaaaggggaa tcagggaggg gagtctcctc ttccagcagc accgcccgct cccgggggag
    10021 agccagccgc tcgcggaccg gttcctcggt caccaccagc cccgtgccgg catcctccag
    10081 aagaagggcc aggcgctccg ccggatgcga ggggtccagg ggcaggtacg ccgctcccgc
    10141 ctcccagatc ccgaggaggc cgatcaccag ggccggcgag cgctctacgg cgagccccac
    10201 caccccctcg gggcctgccc cctgagccgt gagacgcgcc gccaaccgcc gggcgcgacg
    10261 gatcagctcg ccgtaggtca ggctctcgtc cgcgaagcgc accgccacgg catcgggagc
    10321 tatcgccgcc cgctccgcca accgctcggg cacggtgagc ggcgccagag cctcccccgt
    10381 gtcgttccac tccacgagca cctgatggcg ctcggccggc gccaggagcg gcagatccgc
    10441 caccgcccgg tccggcccgg ccacggcttc ggcgaggagc cggagatagt gctccgccca
    10501 gcgccgcacc gtcgtctcgt cgaagaggtc gctgctggat tcgagccgcc cggtccaccc
    10561 tccggcctcc tcggtcaggg tcagcccgag gtcgaactgt gccgtgccgt tgtccaccgg
    10621 cagcggctcg acggcgacgt ccgccagcgc cagcggctcg gcgtcgtcca ggagctggaa
    10681 catctgctgg accagcgggt tgcgcgagag gtcccggacc ggctgcagct cctccaccag
    10741 gcgctctccg ggcagatcct ggtgcgcgta ggcttcgagg gccgtctgcc gcgcctgcgc
    10801 caggaggtcc cggaagccca tcgcctcccc ctgcgtgaag tccgcgcgga ggacgagtgt
    10861 gttgacgaac aggccgatca ggtcctcgac ctctcgccgg ccgcgcaacg cgaccggcac
    10921 ccccacgacc acgtcggcgg ctcccgtgag gcggtggagc aaggtctgga agccggcgag
    10981 gagcgccatg aacggagtcg cccccacctg ccgggcgagg cgtgcgaccc cttccttgag
    11041 ggcggcgggc acggtcagca ccagcgtccg gccgcggaac gtctgcaccg gcgggcgggg
    11101 gcggtcggcg gggaggtcca ggggcacgat cccggccagc ctctccctcc agtaggcgag
    11161 gtgcggtgcc aggagctcct gggtcagcca ccgccgctgc cagaccgcga agtcgccgta
    11221 ctgcaccggc agcgccggca gggacggcag ggctccttgc cggaaggctc cgtagagctc
    11281 ccgcagctcg tgcgccagca ggctcagcga ccagccgtcg gcggcgatgt ggtggatgtt
    11341 gaccgccagc gcgtggaccg cggggtggtc cccctccagg tggatcagaa cggcccggag
    11401 gagcggcccg cgcagcaggt cgaagcgcca acccgcctcc tcgcggagca ggcggcggct
    11461 ctccgcctcc cgggccacgg cggggagggc tcggaggtcg atccgcggca gggtgaatgg
    11521 ggccggcggc agcacgcgct gtaccggccc gtcgtctctc agtccgaaca ccgcgcgcaa
    11581 cgcctcgtgg cggcgcagca cttcgccgaa ggcgcgctcg agggcggcct cgtccagcgc
    11641 ccccgccagc cgccaggctg cgggcaggtc gtaggccggg agccccggcg ccagccggtc
    11701 gaggaagaag aattgctcct gcatgaggga gagcggcagc tccccctcgt ggtccaccgg
    11761 ctcgatgggc gcgaaggagg acgacgcggc gcccctctcc ccactcgcca gcgtctccag
    11821 ccgggcggcc agcgaggcga cggtgggatg gccgagaacc tcggcgccgg acagctcgac
    11881 tcccagcccc tcccgcagac gggagaccag gcgcgtcgcc aggagcgaat gcccgccaag
    11941 ctcgaagaag tcgtcgtgga cccctacatc ctcgactccc agcagctcgc gccagaagcc
    12001 ggccagcctc tcctccaccg gcgtccgggg ggcgacgaag gccgcgcccg ctccggtgct
    12061 ctccccctgg agggccgtga gcgccgcgcg gtccagcttg ccgttgaccg tacggggcag
    12121 ggcggggacg acggtgaacg ccggcggcac catgggctcg ggcaggctcc tccgcaggga
    12181 ccgccgcagc tcgcgcgccg gcgggatctc caggccgggc tcggccacca ggaaacccac
    12241 cagccggcgc tccccggacg gaccttccag cgcggccacc gccgcctccc gcaccgacgg
    12301 gtgccgcgag agagccgcct ccacctcccc cagctcgatg cgaaagccgc ggatcttcac
    12361 ctgctggtcg atgcgcccca gatacaggag gtcaccgccc ggcagccggc ggaccaggtc
    12421 ccccgtcctg tacagccgct cgcccgggct cgcggcccag ggatcgggga cgaaccgttc
    12481 ggccgtcagg tcgggacggt ccagataacc ccgggccagc ccctccccgc cgagatagag
    12541 gtccccctct tcgccagccg ccacctgccg cggatcgatt tccggaccgg ccaccagcac
    12601 ccaagcccgg gtgttggcca ccggccggcc gatgggcggg tgcgcgctgc cgggagggat
    12661 gagggcgaaa gtcgagtagg tggtgtcctc ggatgggccg tagaggttga agacccgctc
    12721 caccccggcc cggtagaggc catccaccag cgcgcccggc agggcctcgc cggcgaggtt
    12781 gaccgtgcgc accgacggcg gcaccgctcc ggagcgcagc agctccgcca tcgccgaggg
    12841 gacggtgttg accagcgtca ccggccgcag ccatgccgcc tccgggagag ccagggcatc
    12901 ccgcgccagg atcaccgtgc cgccgcgcga cagggggacg aacagctcga agacggagag
    12961 gtcgaagcag aaggaggtgg cggccagcac cccttcgagc tcctccaggg tgaagacctg
    13021 ccgtgcccag gcgaccagcc ccgcggcgga gcggtggcgg atcatcaccc ccttcgggcg
    13081 gccggtggag cccgaggtat agatgacgta ggccagtccg tcgggcaacc cgccagggag
    13141 cggccgggac gccggccggt ccgccagcgc acgggcgtcc gcgtccagcg acacgatcca
    13201 tcccgggcca gggggcagcg cggagcgccg gctctcgtcg gtcagcacgc cggcggcccg
    13261 ggagtcctcc agcatgaacg ccacccgctc cgccggatag gccgggtcca gcggcacata
    13321 ggcgcccccc gccttgagga ccgccaggat cgagaccacg gtctcggccg agcggcccag
    13381 gaagacgccg acgcgaacct cgggcccgac accgagggag atcaggtgat gggcgaggcg
    13441 gttggcccgc gcttccagct cacgataggt cagcgagagg tctgccgaaa cgagggccgg
    13501 ggcctcgggc gtgcggtccg cctgctcctc gaacgtctcg tggagccggg ccggtcccgg
    13561 ctcctcgctg tcgagagaac ggaaggaagg gaggggcgtg ggctcgagag agatcatcag
    13621 cgactgctcg agccccgctc aggtccccgg gaccagccgg agcgtcttca ccgccggccc
    13681 cggcaggaac ggcgtgggct tgccctccgc ccaggcgtcg aagccgtcgg cgtagtgctc
    13741 ggacagcgga tggccgctct ggccgcccgg catgtggaag tagccctctt gctcccggcc
    13801 cggcgaaacc accatgcgca acgtcggccc gaagcggacg gtcatcacgc gcggcatctc
    13861 ggcgtcgccc gacatgggcc ggcgcggcat gtccagccag cgtccgagga acggcaccgc
    13921 ctggctgacg gggtgctgga tgcggacggt gttgaactct ccccaggtgc gctcctggac
    13981 cggaccggga gggagctggg cgagcatggt gtccaccgcg gcgaggaact gctcctgcca
    14041 actcttgtac tcgggattca acaaatgggc cggttgctgc gtcaccacgg cccagagcgg
    14101 ctcctcggcg gcatggaagg cgaacaggta ggggaaatcc tcgggcagcc cgccgcagcg
    14161 ggtgaggagg ggctggaaca cggactgcga caggtacatc cggtagccgc gcaccatccg
    14221 gtagccggcg gactccgccg ccgcccggcc gccccagttc tccaccgcct cccgcatctc
    14281 gccgcggcgc ggatcggccg ccaccgcctc gggagtgagc acccccagga gctggtcgcg
    14341 ccagcgctgc aggaacaggg cccggtcgtc gagctggatc ctgcgcatgt cctcgaccgt
    14401 ggccttctcc acggcgaaga ggccgtcgcg gatctgccgc gcccgcgcgc cgtggatatg
    14461 gatgcggccg ccgatcttct ccatcatctc ggggctggcg ctgcggttgt tgccgttcca
    14521 cagccggccc gatccgggat cgacgatctg cggcatctcc tcgggaggca gcaggccgtc
    14581 ccagcgccgc gacccgtcgg cccaggagcc ggccaccacg ccgtcgaagc cgacgcgccg
    14641 cggcacccgg cccaggatcg tccaggcgat ccggccgccg gcatcggcga agaagacgtt
    14701 caggaccggc ac
//
LOCUS       DASPJI010000714         5668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_102597, whole genome shotgun sequence.
ACCESSION   DASPJI010000714 DASPJI010000000
VERSION     DASPJI010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102597"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2036)
                     /locus_tag="VEL74_20055"
     CDS             complement(<1..2036)
                     /locus_tag="VEL74_20055"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64886.1"
                     /translation="MLHHPLRLIASLLLLLIPVPLAAATLDDEMEAYLRVVAVSGREE
                     PAAAFVVQRLAGLPVSRDALRNVVVTLGSGTPRRLVTCPLDEPGYVVSRILEDGYLRL
                     VPSGNAQLGNLWDQSHEGQVMVVGGSQGWRAGAVALPSVHLQQAPPPAGEPVPFTIYD
                     ADVDVGAESAAEVAEMGIRLLDPVALVRRPVRLPGGRIAGPAARAKGACLALVDAARR
                     FHRTPGSGTTVFAWTAGDLLNPAGLAHLARERGPFDEVLQISRGFEWDDQGRPQPIPG
                     PGGLLAAGELPPALSRVRMATPPQPGGFGRPDWGQARVGFLALPGLYPGTPVETVAVN
                     DVRRLADTLLEIAGSAAAPSGALPPLAAPEPLLQSEEHAETSKLLGALIARYGVSGAE
                     GPVREEIQKHLPAWARPVTDAEGNLRVTFGPADSREHVLLVAHMDEVGFRVAEVLADG
                     RLRLQTRGGLLPTAWEAQAALVHGDKGSVPAVFEPRQDWRSAERRNLQGPLTAWLGTA
                     SAQETAALGIAAGSTVTMPKELLRVGRHRVVARSFDDRSGSTALLLALRRIDPAKIRK
                     KVTVAWSVQEEVGLVGAEALARDLPDLTRVHPVDTFVSSDSPVETRRFAHAPLGKGMV
                     LRTMDNAYLAPRELIDRYVAFAARHRIPVQYGFTGGASDGVAFLRQGVTMLPFS"
     gene            2092..2772
                     /locus_tag="VEL74_20060"
     CDS             2092..2772
                     /locus_tag="VEL74_20060"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HYG64887.1"
                     /translation="MARPLLDETFCIPAEARDLEGFRVWARSPRFPERGRLDYLGGDI
                     EVDMSPEDLYTHGTLKIEIAALLHALVTKSGTGVVFTDRTRISSPPADLSVEPDVVAV
                     LWRTLDSGRLREIPSAGERPGRYIELEGAPDLVVGIVSDGSVGKDLKRLPSFYAAAGV
                     PELWVADARGSEIRFEIRALDSGLYKKVEPNPDGWLMSPFLGLRVRLSRKRVHLDRGV
                     YELETQGN"
     gene            2787..3677
                     /locus_tag="VEL74_20065"
     CDS             2787..3677
                     /locus_tag="VEL74_20065"
                     /inference="COORDINATES: protein motif:HMM:NF024098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HYG64888.1"
                     /translation="MSYLLFLDESGHDHRTTPYEVRGGVALHASELWSFVQDMQRLEL
                     ASFGAELHQFQTELKGCKLLDKDRFKWAAQEEPMSDEERRKHCRGFLTRGLEKRAPLR
                     SEFTAYGQACLEMATGIFELLRQHKALLFAAAIPRSVVKPDTYEAEEYLRKDHVFLLE
                     RYFYFLEQKRQHGLLVMDQVEKDQDRRFVRRLEAYFRKTRLGRYRSAWIVPAPFFVSS
                     DMAYPVQAADLAIYCVNWGFRLPTRGMDAPVRREIADHYGRWLAQLQFHGEGYRDGAV
                     YDSYGIVFVPDPYAAKQGGE"
     gene            complement(3861..4400)
                     /locus_tag="VEL74_20070"
     CDS             complement(3861..4400)
                     /locus_tag="VEL74_20070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64889.1"
                     /translation="MRSFTDGSSPSNVSNGPNAFTASFLDRLAERDEGPTAPEADVAG
                     PWRIEEIPGEGFGVFRLGESTARGFEPTLLCPSEWLALVAAGVLPGTGRDPMLLLQKD
                     RNPDGTFSLILESGEVVGRSRLFDEKLVDSMNTAIHLLRAPESLANVLDAAGLSPLER
                     CGAILDERAGQRAGRVERK"
     gene            5360..5536
                     /locus_tag="VEL74_20075"
     CDS             5360..5536
                     /locus_tag="VEL74_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64890.1"
                     /translation="MKKKTKKLVLTKETVQGLDADLAQAVGGSTPQAGCTGVVCPYSA
                     PYYCPRMPAGTVNP"
BASE COUNT          970 a         1930 c         1928 g          840 t
ORIGIN      
        1 caggagaagg ggagcatggt cacaccctgg cggaggaagg cgacgccgtc actcgccccg
       61 ccggtgaagc cgtactggac ggggatgcgg tggcgggccg cgaacgccac gtagcggtcg
      121 atcagctcgc gcggcgccag gtaggcgttg tccatggtgc gcagcaccat ccccttcccg
      181 agcggggcat gggcgaagcg ccgggtctcg accggcgagt cggaggagac gaaggtgtcg
      241 actggatgga cccgggtgag gtcgggcagg tcgcgggcca gggcctcggc gccgaccagg
      301 ccgacctcct cctggacgct ccaggccacg gtcaccttct tgcggatctt ggccggatcg
      361 atccggcgca gggcgagcag cagggcggtc gagcccgagc ggtcgtcgaa gctccgcgcc
      421 accacccggt gccgcccgac gcgcagcagc tccttgggca tggtgacggt gctgccggcc
      481 gcgatcccga gcgccgcggt ctcctgggcg gacgcggtcc ccaaccaagc ggtcagcgga
      541 ccctggaggt tgcggcgctc cgccgaccgc caatcctggc gcggctcgaa gaccgccggc
      601 accgatccct tgtcgccgtg gaccagcgcc gcctgcgcct cccaggcggt cggcagcagc
      661 ccgccccggg tctgcaggcg gaggcgcccg tccgccagga cctcggcgac ccggaaaccc
      721 acctcgtcca tgtgcgcgac cagcagcacg tgctcccggc tgtccgccgg gccgaaggtc
      781 acccgcaggt tgccttcggc gtccgtgacc gggcgggccc aggcgggcag atgcttctgg
      841 atctcctcgc gcaccggccc ttcggcgcca ctcaccccat aacgggcgat gagggcgccg
      901 aggagcttcg acgtctcggc gtgctcctcg ctctgcagga gcggctccgg ggcggcgagg
      961 ggagggaggg cgccggaagg cgccgcggcc gatccggcaa tctcaagcag ggtatccgcg
     1021 agccggcgga catcgttgac ggcgacggtc tcgaccggcg tgccggggta gagcccgggc
     1081 agggccagga agccgacacg ggcctggccc cagtccggcc ggccgaagcc gccgggctgc
     1141 ggtggcgtgg ccatgcggac ccgcgacagc gccggcggca gctcccccgc cgcgagcagg
     1201 ccgcccggcc cggggatcgg ctgcggccgc ccctggtcat cccattcgaa gccgcggctg
     1261 atctggagca cctcgtcgaa cgggccgcgc tcgcgggcca ggtgcgcgag gcccgcgggg
     1321 ttgaggaggt cgcccgcggt ccaggcgaag accgtggtgc cggagccggg cgtccggtgg
     1381 aagcgccgcg cggcgtcgac cagcgccagg caggccccct tggcccgcgc ggccggtccc
     1441 gcgatccgtc ctcccggcag ccgcacgggc cggcgcacca gcgccaccgg gtcgagcagc
     1501 cggatgccca tctccgccac ctcggccgcg ctctcggcgc cgacgtcgac gtcggcgtca
     1561 tagatggtga aggggacggg ctcgccggcg ggaggagggg cctgctgcag gtggacggag
     1621 ggcagcgcca ccgcgccggc ccgccagccc tgggagcccc ccaccaccat gacttgcccc
     1681 tcgtgggact gatcccacag gttccccaac tgcgcgttgc ccgaggggac gagccgcagg
     1741 tagccatcct ccaggatgcg gctcacgacg tagcccggct cgtccagcgg acaggtgacc
     1801 agccgccggg gcgtgccgga gccgagcgtg accaccacgt tcctcagggc gtcgcgggag
     1861 accggcagcc cggcgagacg ctggaccacg aacgccgcgg ccggctcttc gcggccggag
     1921 acggccacca cgcggaggta ggcctccatc tcgtcgtcca gggtcgcggc ggcgagaggg
     1981 acggggatga ggagcaggag cagggatgca attaggcgca gggggtggtg gagcatcctc
     2041 tgaagctatc actcggacca tgctacgttc gcccctggaa ggagaccggc catggcaaga
     2101 cccctgctcg acgagacgtt ctgcatcccg gcggaggcac gcgacctcga aggcttccgt
     2161 gtctgggcgc gctcgccccg cttccccgag cggggccgcc tcgactacct cggcggcgac
     2221 atcgaggtgg acatgagccc ggaggatctc tacacccacg gcaccttgaa gatcgagatc
     2281 gcggccctgc tccacgccct ggtcaccaag agcggcacgg gcgtggtgtt tacagaccgc
     2341 acccggatca gctcgccgcc agccgacctc tccgtcgagc cggacgtcgt cgccgtcctc
     2401 tggaggaccc tggacagcgg caggctgcgg gagatcccat ccgcgggcga acgtcccggg
     2461 agatacatcg agctcgaagg cgcgccggac ctcgtcgtcg ggatcgtgag cgacggctcg
     2521 gtgggcaaag acctcaaacg gttgccatcc ttctacgcgg cggccggcgt cccggagctc
     2581 tgggtcgccg acgcccgagg gagcgagatc cgtttcgaga tccgggcgct cgattccggc
     2641 ctttataaga aggtggagcc caaccctgac ggctggctca tgtccccctt cctcggcctc
     2701 cgggtccggc tcagccggaa acgggtgcat ctggaccgcg gggtctacga gttggagacg
     2761 caggggaact gagaggggcc agaggaatga gctatctact ttttctcgat gagagcggcc
     2821 acgaccatcg aactacgcct tacgaagttc gcggtggcgt ggcgctgcat gcttcggagc
     2881 tttggtcctt tgttcaggat atgcagcgac tcgagctcgc ctcctttggt gccgagctgc
     2941 atcagtttca gaccgaactt aagggctgca aactcctgga taaagaccga ttcaaatggg
     3001 cagcccagga ggagccgatg tccgacgagg agcggcggaa acactgccgc ggctttctca
     3061 ccagaggact ggaaaaaagg gcgccgcttc gctcggagtt cacggcctat gggcaggcat
     3121 gcctggagat ggcgacggga atattcgaac tgctcaggca acacaaggca ctcttattcg
     3181 ccgctgctat cccgcgcagc gtcgtcaagc ctgacaccta cgaagcagaa gagtacctcc
     3241 ggaaagacca tgtcttcctt cttgagcgtt acttctattt tcttgagcag aagaggcagc
     3301 acggtctgct cgtaatggat caagtggaga aggaccaaga ccgccgcttc gtgcggcgcc
     3361 tcgaggcata cttccggaag acccgactag gacgttaccg gtcagcgtgg atcgtaccgg
     3421 cgcccttttt cgtctcgtct gacatggcct acccggtcca ggcagcagac ctggcaatct
     3481 attgcgtgaa ctggggcttt cgtctgccga ctcggggaat ggacgcacct gtccgacgag
     3541 agatcgccga ccactacggt cgatggctcg cccagcttca attccatgga gaggggtatc
     3601 gcgacggggc cgtctacgac tcctacggca tcgttttcgt accggacccg tatgcggcca
     3661 agcaaggggg agaatgaaaa ggaggcaacg ccgtcagagc cacccgaaag cagcccctgg
     3721 gccgagcctc caccagaagt atcggcgatc catgtcttga agtcaagggg cttaagaacc
     3781 aaaagggccc tcatagcatc tttatgcccg cgtcccgggc ggcccggatc ccctccgggc
     3841 cgcccctgtg actctcctgt ctacttcctc tcaaccctcc ccgcccgctg accggcccgc
     3901 tcgtcgagga tggcgccgca acgctccagc ggcgagaggc cggcggcgtc caggacgttg
     3961 gcgagcgact ccggcgcccg cagcaggtgg atggccgtgt tcatggagtc caccagcttc
     4021 tcgtcgaaga gccgggaccg gccgaccacc tcgccgctct ccaggatgag agagaaggtg
     4081 ccgtcggggt tgcggtcctt ctggagaagg agcatcgggt cccggccggt gccggggagg
     4141 actcccgcgg cgaccagggc cagccattcg gacgggcaga gcaacgtcgg ctcgaagccg
     4201 cgggccgtgc tctcgccaag ccggaacacc ccgaagccct ccccggggat ctcctcgatc
     4261 cgccacggac cggcgacgtc cgcctccggc gcggtcggac cctcgtccct ctcggccaga
     4321 cggtcgagga acgaggcggt gaaggcgttg ggcccgttgg aaacattgga cggactgcta
     4381 ccatcagtga agctacgcat aagggttcct ttcccttttg tgtggcgagg tccgggaggg
     4441 aggtaccact cccacccggg ccgccacacg gtgtctgtgg aaccgcggcc ggcaccgcgc
     4501 cggaccacgg gcgctgcgta caacctgata accggaatcc tattacgaag acgcggcaga
     4561 agtcaacctt ctggcgggat cttctcgccc tctccagccg ctcccgtctc cccggagcac
     4621 gatccaagcg cggcgccgcc cggcccggcg ccgcgccctg acgtgtacgt cacttgtcaa
     4681 agaaccaaaa ggatggagcc ccccaacgac tagggctccc gcctggggag ggagactacc
     4741 acacaatcac catcgctgac catgttgaca tctgcggagc gaggaccgca ggatcagcct
     4801 gctttgctga tcctggagcc tcggcaacaa tcctggctcc cagacccctg gatctcatcc
     4861 aagccgttgg gacgcgatcc aggacgtccg gacttcttcc caagcgcctg gacgccttcc
     4921 agaccgtctg gatgcgctcc gtggcacccg ggaagcgatc caacgcacct ggacgcgatc
     4981 cagccggccg gggaagcgtc cggagcgttc ggaaggggtc cgagccgcgc gggagcgccg
     5041 ctctttgaca actgaatcgt gacgggggtg ccgccgtgct caaggcggcg ccccatgaac
     5101 tcctggaccg tgggtgtagg gcaccacctg cggtcatttc aagcgggcgt gctatttcta
     5161 gacgccagag ctttgggatg agccaccggc aggcacccgt ccaccggtgg ctgggttcga
     5221 cgaggaggcg aaggcctgat gtgttcgccc cctgcattta ttagcaataa ttcacatttg
     5281 gacagagtca tcgccttccc caccggcagg gtagaatggg ggccgcgcca cagggcgcgt
     5341 tactgacgaa ggagtggcga tgaagaagaa gacgaagaag ctggtcctga cgaaggagac
     5401 ggtgcagggc ctggatgcgg acctcgcgca agctgtcggc ggatcgaccc cgcaggccgg
     5461 ctgcacaggc gtggtgtgcc cctacagcgc cccctactac tgcccaagga tgcccgcagg
     5521 cacggtcaac ccgtgagtac acggccctct ggcttccacc agagggcctc tccaccaccc
     5581 aagggcagac gagacgtgcc ccctcatcca ccgcaattcg gcggggacgc cgcggccgga
     5641 ggcgaaccca tgtggatcat gatcgcgg
//
LOCUS       DASPJI010000715         3441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_677976, whole genome shotgun sequence.
ACCESSION   DASPJI010000715 DASPJI010000000
VERSION     DASPJI010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677976"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1408)
                     /locus_tag="VEL74_20080"
     CDS             complement(20..1408)
                     /locus_tag="VEL74_20080"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HYG64891.1"
                     /translation="MINPRFRSRPVAPRLGAVLLLLASLAAVPLLGAEARRGEVLVIR
                     VDTVIHPVAVELVQDAVREADAAGAAALVVEINTPGGLMSSTREITTAMLGARTPVVA
                     YVAPGGAQAASAGFFILMAADVAAMAPGTNAGAAHPVDGGGGDIPGVMGEKAAQDAAA
                     NIRALAARNGRNPELAQAAVLESRSFTADEAVRARLADLVAPSLPRLLEALDGRRVRR
                     GETVVTLRTAGAPVRNLELSPFRRFLGVLATPDIAYILLTLGFLGLYFELTTPGAILP
                     GVIGGICLLLGFFALSVLPVNYAGLGLLFLAMIFFIAEIKVVSHGMLAVAGVISMVLG
                     SLMLFKSPDPALRVSLELIATLALFSLAVVGFLIFMARRALRAPVRTGREGLVHEVGT
                     ARSDLAPHGKIFVHGEIWEAVAEEPVRAGEPVEIVGIRDLVLAVRPYRGASSASSASS
                     DSTVGTARLSAP"
     gene            complement(1439..>3441)
                     /locus_tag="VEL74_20085"
     CDS             complement(1439..>3441)
                     /locus_tag="VEL74_20085"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HYG64892.1"
                     /translation="ATQILTEHGGWPNSVFLTPDLKPFYAGTYFPPDDRYGRPGFRKV
                     LEDLSHAWKTRRSDVEEQAEEMARAMRHYLEERTRPAAAPPDAEAASRSVDALARRFD
                     REWGGFGGAPKFPTPSNLYLLLEMAGERPEAGEMLAATLDQMARGGLYDHLGGGFHRY
                     ATDREWKIPHFEKMLYDNGFLLELLAREHVRTGDPQAARAARETAAWIAREMTSPEGA
                     LWSAIDAETHGHEGAYYIWTREEIDALIGSEDAEFLAPLLGFAGPPFFEDQYYVLHLP
                     ERLEDAARRRRMPEADLLREVEALKARLFEARGRRERPLTDDKILADWNGIAIAGLAV
                     AGQLLGEPDLVARAARAAEFVLTRMRPAGGPLLHSWRQGQGKVVAMLGDYAFLVRGLL
                     ALHTATGEPRWLGAAAELTEEQIRRLRDVQGGFFVAGESSDILFRSKDVFDGATPAGN
                     AVAILNLLDLAERTGEGVWRTEARAALQAFASLVEGYPDAVRMLTIAARRFHRAEGSW
                     GPAVAPVSMEHGVEERAAHEREATAAGVFKLEREAEHLVTPRLELGEVAEGGEDGWRP
                     FRLALEIAPGWHIHGNPASEAYLVATEVRAEGASLRGVRYPEADELQPGFADKALAVY
                     ERTVEITGEVGEVSSGGRLLVTFQPCDDTRCLPEATVEMVIR"
BASE COUNT          492 a         1265 c         1251 g          433 t
ORIGIN      
        1 ctactcccct ctcttctgtt tacggagcgg agagccgcgc cgttcctacc gtgctatccg
       61 aagacgccga agaagccgaa gaagccccgc ggtagggccg caccgccagg accaggtccc
      121 ggatgccgac gatctccacc ggctcccccg cccggaccgg ctcctccgcc accgcctccc
      181 agatctcgcc atggacgaag atcttgccgt gcggcgccag gtcggagcgg gccgtcccga
      241 cctcgtgcac cagcccctcg cggccggtgc gcaccggagc gcgtagcgcc cggcgggcca
      301 tgaagatcag gaagcccacc accgccaggg aaaatagcgc cagggtggcg atcaattcca
      361 ggctgacgcg cagcgcgggg tcggggctct tgaacagcat gagcgagccg aggaccatgg
      421 agatcacccc ggccacggcg agcatcccgt ggctcaccac cttgatctcg gcgatgaaga
      481 agatcatggc caggaagagc agccccaggc cggcgtagtt gaccggcagc accgagaggg
      541 cgaagaagcc gaggagcagg cagatccccc cgatgacgcc ggggaggatg gcgcccggcg
      601 tcgtcagctc gaagtagagg ccgaggaacc ccagggtgag caggatgtag gcgatgtcgg
      661 gggtggccag cacccccagg aagcgccgga acggcgacag ctccaggttg cggaccggcg
      721 cgccggcggt gcgcagggtg accaccgtct cgccgcggcg gacccggcgc ccgtcgaggg
      781 cttcgagcag ccggggcagg ctcggcgcca ccaggtcggc gaggcgcgcc cggaccgcct
      841 cgtcggcggt gaacgagcgg ctctcgagga ccgcggcctg ggccagctcc gggttgcggc
      901 cgttgcgcgc cgccagcgcc cggatgttgg cggcggcgtc ctgcgccgcc ttctcgccca
      961 tcaccccggg gatgtctccg cccccgccgt cgacgggatg ggcggcgccg gcgttggtgc
     1021 ccggggccat ggcggccacg tccgcggcca tcaggatgaa gaaccccgcc gaggcggcct
     1081 gggcgccgcc gggggcgacg taggcgacga ccggggtgcg ggcccccagc atggcggtgg
     1141 tgatctcgcg ggtggagctc atgaggccgc ccggggtgtt gatctccacc accagcgcgg
     1201 cggctccggc ggcatccgcc tcgcgcaccg cgtcctgcac cagctccacc gccacgggat
     1261 ggatcaccgt atcgacgcgg atgacgagca cctcgccccg gcgggcctcg gccccgagga
     1321 gagggaccgc cgccaggctg gcgaggagga gcaggacagc cccgagccgc ggggcgaccg
     1381 gacgggaccg aaaccggggg ttgatcattc ttcgagtata cgcccgagcc gcgcgcctct
     1441 accggataac catctccacc gtcgcctcgg gcaggcaccg ggtgtcgtcg cagggttgga
     1501 aagtgaccag aagccggccg ccggaggaga cctcccccac ctcgccggtg atctccaccg
     1561 tcctctcata gaccgccagc gccttgtcgg cgaatcccgg ctgcagctcg tcggcctcgg
     1621 gatagcggac gccgcgcagg ctcgccccct cggcgcgcac ctcggtcgcc accagatagg
     1681 cctccgaggc cgggttgccg tggatgtgcc agcccggggc gatctccagc gccagacgga
     1741 aggggcgcca gccatcctcc cccccctcgg ccacctcgcc cagctccagg cgcggggtga
     1801 ccaggtgctc ggcctcgcgc tccagcttga acacccccgc cgccgtggcc tcgcgctcgt
     1861 gcgccgcccg ctcctcgacg ccgtgctcca tggagaccgg agccacggcc ggcccccagg
     1921 acccctcggc ccggtggaag cggcgcgccg cgatggtcag catgcgcacc gcgtcggggt
     1981 agccctcgac cagcgaggcg aaggcctgga gggccgcccg cgcctcggtg cgccagaccc
     2041 cctcgccggt gcgctcagcg aggtccagca ggttgaggat cgccaccgcg ttgcccgccg
     2101 gggtggcgcc gtcgaagacg tccttgctgc ggaagaggat gtcggagctc tccccggcca
     2161 cgaagaagcc tccctggacg tcgcgcaggc gccggatctg ctcctcggtg agctcggccg
     2221 ccgcgcccag ccagcgcggc tcgccggtcg cggtatggag ggcgaggaga ccccgcacca
     2281 ggaacgcgta gtcgcccagc atggccacca ccttgccctg cccctgccgc caggaatgga
     2341 ggagggggcc gccggccggc cgcatgcggg tgaggacgaa ctccgccgcc cgcgccgccc
     2401 gggcgaccag gtccggctca ccgaggagct ggccggccac ggcgagcccg gcgatggcga
     2461 tgccgttcca gtcggccagg atcttgtcgt cggtcagcgg gcgctcccgc cggccgcgcg
     2521 cctcaaagag cctggccttc aacgcctcga cctcgcgcag caggtccgct tcgggcatcc
     2581 ggcggcgccg cgccgcatcc tccagccgct ccggcaggtg gaggacgtag tactgatcct
     2641 cgaagaacgg cggcccggcg aaccccagga gcggcgccag gaactcggcg tcctccgagc
     2701 ctatcagggc gtcgatctcc tcgcgcgtcc agatgtagta ggccccctcg tggccgtggg
     2761 tctcggcgtc gatggcgctc cacagcgccc cctcgggcga ggtcatctcg cgggcgatcc
     2821 aggccgcggt ctcgcgggcg gcgcgcgccg cctgcgggtc gccggtacgg acgtgctcgc
     2881 gggccaggag ctcgaggagg aagccgttgt cgtagagcat cttctcgaag tgggggatct
     2941 tccactcccg gtccgtggcg tagcggtgga agccgccgcc gaggtggtcg tagaggccgc
     3001 cgcgggccat ctggtccagg gtggcggcca gcatctcccc cgcctccggc cgctcgcccg
     3061 ccatctccag cagcaggtag aggttcgacg gggtggggaa cttgggggcg ccgccgaagc
     3121 ccccccactc ccggtcgaag cgccgggcca gggcgtccac cgagcgcgag gccgcctccg
     3181 cgtcgggggg cgcggcggcc ggccgggtcc gctcctccag gtagtgacgc atggcgcggg
     3241 ccatctcctc ggcctgctcc tcgacgtcgg agcgccgggt cttccaggcg tgggacaggt
     3301 cctccagcac cttgcggaat ccgggacggc cgtaccggtc gtcgggcggg aagtaggttc
     3361 cggcgtagaa tggcttcaag tccggggtga ggaagacgga gttgggccag ccgccgtgct
     3421 cggtgaggat ctgggtggcg g
//
LOCUS       DASPJI010000716         3390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_559089, whole genome shotgun sequence.
ACCESSION   DASPJI010000716 DASPJI010000000
VERSION     DASPJI010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559089"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /gene="fdhD"
                     /locus_tag="VEL74_20090"
     CDS             <1..462
                     /gene="fdhD"
                     /locus_tag="VEL74_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HYG64893.1"
                     /translation="GPELPPDLIYRLPDKLREGQGLFAATGGLHAAALFDVSGHLLAL
                     REDVGRHNALDKLLGWAFLEGRVPLHDHVVLVSGRSSYEILQKCLVAGVPVVCAVSAP
                     SSLAVELAERFGITLVGFLRGGRFNVYSGIERIAAAGRPAARRRSSLPVSV"
     gene            719..1264
                     /locus_tag="VEL74_20095"
     CDS             719..1264
                     /locus_tag="VEL74_20095"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HYG64894.1"
                     /translation="MKTKAFHLAAAIGLVLTGAAGLTVAGCGDRGAVETSRDAEGDPQ
                     LRVDGEQVEENLKETGEALRQGGEQVEQAAEQVGQALEQGAERIEREVGPVAQAVLND
                     AGITAKVKAKLIADPQVNPFHIDVDTLDGVVTLSGKVRSENVKAEAEKLAQITEGVKS
                     VTNNIQVAGDAPTTSPPPAGR"
     gene            1329..1934
                     /locus_tag="VEL74_20100"
     CDS             1329..1934
                     /locus_tag="VEL74_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HYG64895.1"
                     /translation="MNAYIESFLPLFVAINVPGILPMYLGLTETLTPAERRRLTFLAT
                     ATASLIAVLILFAGQLIFNLLGITVNDLRVGGGLILLVLSISNLIFGDYRRRDPLEGE
                     DGEAGADIGIVPLGIPLIMGPAAIASVLVSREAYGYLPTLTSLVINMTLVYLVFAFGP
                     YFARFVGPAVSRAVAKVASLFLAAISVAMIRAGIVGMLAAM"
     gene            complement(1936..2517)
                     /locus_tag="VEL74_20105"
     CDS             complement(1936..2517)
                     /locus_tag="VEL74_20105"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HYG64896.1"
                     /translation="MKLLAPALLLGFLFLAGCGSPVAVLRHNVRYLELLRQPSPEWLP
                     VPVEGVSAARLYDSWGDARDGGARHHEGIDIFAKRNTPVRSATAGVIEAKAERGLGGR
                     TVFITGPGGYRHYYAHLEAWGEQAVGDWVEAGDVIGYVGNSGNAAGGPTHLHYGIYAP
                     VGGAINPYPLLKAVPPPPPPPAPATPATIAEAG"
     gene            2521..2964
                     /locus_tag="VEL74_20110"
     CDS             2521..2964
                     /locus_tag="VEL74_20110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYG64897.1"
                     /translation="MKNPSGRRTLPGVRSRHGFSLIEALVALLILEMALLMGLALLLE
                     QPRIVSRLDRQRQALRSLESALELVRAGAIPLETGPVGTWLELAGAPPPAGTTVWLEV
                     EPAALPSSPSPPGLFHVTVRARWTAAGGLRTREVETLVWSPDAAP"
     gene            2951..>3390
                     /locus_tag="VEL74_20115"
     CDS             2951..>3390
                     /locus_tag="VEL74_20115"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HYG64898.1"
                     /translation="MRHPDRVRGSRAAGFTLVELLVSLLLLLLALGLAAQVLVESSQM
                     LADAAAEQVESPPSLAVARLRADVLGSASFSALEDNLLLQGHPAGSVLYELAGDELRR
                     IVLDTTGEPLGESVLLRGVIGWRCSEVGPRLLAVTIRYRRRAVRP"
BASE COUNT          482 a         1211 c         1170 g          527 t
ORIGIN      
        1 gggccggagc tgccgcccga ccttatctat aggctgccgg acaagctgcg ggaggggcag
       61 gggctcttcg ccgccaccgg cggcctgcat gccgccgccc tgttcgacgt ctcgggtcac
      121 ctcctcgccc ttcgggagga cgtggggcgc cacaacgccc tcgacaagct cctgggctgg
      181 gccttcctgg agggccgggt ccctcttcac gatcacgtgg tcctggtgag cggccggtcg
      241 agctacgaga tcctgcagaa atgcctggtg gcgggggtac cggtggtctg cgccgtctcg
      301 gcacccagca gcctggcggt ggagctggcc gagcgcttcg gcatcaccct ggtcggcttc
      361 ctgcgcggcg ggcgcttcaa tgtctacagc ggcatcgaga ggatcgccgc cgccgggcgg
      421 cccgcggcac ggcgccgctc ctccctgccg gtctccgtgt aacctttacc cgcccggcag
      481 ggccggcagg gaagccgccg caggaagacc cgggggaaag attggttcct tcccgagcgg
      541 agcgcggcct tggacacttg gcacgcaaga tgcactataa gcaggacaag aggcctttcg
      601 cggcccctcc ctgaaaaggg gaggcgtcct gaacccaagt ggatcagaat acgcctcccc
      661 ccttttttta ttcgggcagg gagatctcaa tagcagcacg gacgaggagg acgacgccat
      721 gaagaccaaa gcgtttcatc tggcggcagc gatagggctg gtcctgaccg gagccgccgg
      781 cctcacggtg gccggctgtg gggaccgggg agcggtggag acgagccgtg acgccgaggg
      841 cgatccccag cttcgcgtcg acggcgagca ggtcgaagag aacctgaagg agaccggcga
      901 agcgttgcgc cagggaggcg agcaggtgga gcaggccgcc gagcaggtcg ggcaggctct
      961 ggagcagggg gccgagagga tcgagcggga ggtcgggccg gtggcgcagg cggtgctgaa
     1021 cgacgccggc atcacggcca aggtcaaggc caagctcatc gccgaccccc aggtcaaccc
     1081 cttccacatc gacgtcgaca ccttggacgg cgtggtcacg ctgagcggca aggtgaggtc
     1141 cgagaacgtg aaggccgaag cggagaagct ggcccaaatc accgagggcg tcaagagcgt
     1201 caccaacaac atccaggtgg cgggggatgc cccgaccact tcgccgccgc ccgccggccg
     1261 ctgagccggc tccaggcgtg tagaatcctc tccggccgcg ggtctcctcc cgcggccgtt
     1321 tccattccat gaacgcctac atcgagagct tcctgccgct cttcgtcgcc atcaacgtgc
     1381 ccgggatcct ccccatgtac ctggggctca cggagaccct cacccctgcc gagcgccggc
     1441 ggctgacgtt cctggccacg gccacggcct cgctcatcgc cgtcctcatc ctcttcgccg
     1501 gccagctcat cttcaacctc ctcggcatca ccgtgaacga cctgcgggtc ggcggcggcc
     1561 tgatcctgct ggtcctcagc atctccaacc tgatcttcgg cgactaccgg cgccgcgacc
     1621 ccttggaagg ggaggacgga gaggccggcg ccgacatagg catcgtgccg ctgggtatcc
     1681 cgctgatcat gggacccgcg gccatcgcct cggtcctggt gtcacgggag gcctacggct
     1741 acctcccgac gctcacctca ctggtgatca acatgaccct ggtctacctg gtgttcgcct
     1801 tcggtcccta cttcgcccgc ttcgtggggc cggcggtgag ccgcgccgtg gccaaggtgg
     1861 cgagcctctt cctcgccgcc atctcggtcg cgatgatccg cgccggcatt gtcggcatgc
     1921 tggccgcgat gtaggtcagc cggcctcggc gatcgttgcc ggcgttgccg gagccggtgg
     1981 tggaggaggg ggcggcaccg ccttgagcag cggatacggg ttgatggcgc cgcctaccgg
     2041 ggcgtagatc ccgtagtgca ggtgggtggg gccgccggcg gcgttgccgg agttgccgac
     2101 atagccgatc acgtctccgg cctccaccca gtcacctacc gcctgctccc cccacgcctc
     2161 cagatgcgcg tagtaatgac ggtagccgcc cgggccggtg atgaagacgg tccgccctcc
     2221 gagcccgcgc tccgccttgg cctcgatcac cccggcggtg gcgctgcgca ccggggtgtt
     2281 gcgcttggcg aagatgtcga tcccctcgtg atggcgcgcg ccgccgtcgc gggcgtcgcc
     2341 ccaggagtcg tacaggcgcg cggccgagac cccctccacc ggcaccggca gccactccgg
     2401 cgagggctgc ctcagcaact cgagataacg cacgttgtgg cggaggacgg cgaccgggct
     2461 gccgcagccg gcaaggaaga gaaaaccgag gaggagagcg ggggccagga gcttcacgtg
     2521 gtgaaaaacc cgtcaggccg ccgtactctt cccggcgtga gatcccggca tggcttctcc
     2581 ctgatcgagg ccctggtcgc cctcctgatc ctggagatgg cgctgctcat ggggctggcc
     2641 ctcctgctgg agcagccgcg catcgtgagc cggctggacc gccagcgcca ggccctccgg
     2701 tcgctcgagt ccgccctgga gctggtccgg gcgggcgcga tccctctcga gaccggcccc
     2761 gtcgggacct ggctggaact cgcgggcgcg ccgccgcccg ccggcaccac cgtctggctg
     2821 gaggtcgagc cggccgcgct cccgtcgtcg ccaagcccgc cgggcctctt ccatgtgacc
     2881 gtccgtgccc gttggaccgc cgccggcggc ctgcgcaccc gcgaggtcga gaccctggtg
     2941 tggagccccg atgcggcacc ctgatcgggt ccgggggagc cgcgcggccg gcttcaccct
     3001 cgtcgagctc ctcgtctcgc tgctcctcct gctgctcgcc ctggggctcg cggcgcaggt
     3061 cctggtggag tcgtcccaga tgctcgccga tgccgcggcc gagcaggtcg agtcgccgcc
     3121 ctccctggcc gtggcccgcc tccgcgccga cgttctcggc tcggcctcgt tctcggctct
     3181 cgaggacaac ctgctcctcc aagggcaccc agccggcagc gtcctctacg agttggccgg
     3241 cgacgagctg cgccggatcg tgctcgacac caccggcgag ccgctcggcg agagcgtgct
     3301 gctgcgcggt gtcatcggct ggcgatgcag cgaggtcggg ccccgtctcc tggcggtgac
     3361 gatccgctac cgacgccgcg ccgtccggcc
//
LOCUS       DASPJI010000717         6999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_392664, whole genome shotgun sequence.
ACCESSION   DASPJI010000717 DASPJI010000000
VERSION     DASPJI010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392664"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3353
                     /locus_tag="VEL74_20120"
     CDS             <1..3353
                     /locus_tag="VEL74_20120"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64899.1"
                     /translation="AAELAEAIAFMVQEAAGGSAEGVAAVPAIGPSPLRAAGGPFPLS
                     FAQERIWFLDQLEPGTAAFDLPAATRLQGRLDRAALEHSLNEVVRRHETLRTRFLTVD
                     GQVVQEVLPSLDLDLPMADLSALPGESRAAEERRLVLDHASRPFDLANGPLAAFLLLR
                     LTPEDHLLALVMHHIISDGWSLGVLQSEVARHYGRALLPVSEPLPELSFHYADYADWQ
                     RRHFQGDVLERELAYWRPRLHGVPFLELRGDRPRPPVQTFRGGYEDFTLPAGLARSLE
                     GLAQREGSSLFMVLLTGFDLLLQRHAQQDDFAVGALIAGRNRAELEGMIGCFVNNLAL
                     RTDLSGEPDGRELLRRVRETTIEAYAHQDVPFEKLLEELQPERDLSRGALIQVMFNLL
                     NFPTVHEELPGLTMSSSGVRGERANFDLTLWMTEARDGLVGWLEYNADLFDRATARRM
                     TAHLRRLLEGLAADPGQRVAELPLLGEAERRQVLVEWNRTGRERPWEEGFLGLFQARV
                     ESVPGDVAAVCQGESLTYAELGRRSRRLAAWLASEGVGPESVVPILADRGLDFLAAVL
                     GVLQAGGAYLPLDPNQPASRAVRVLRQSGAALVLVGSDHALRLVAASEGGALPDIVAF
                     EGLLRDEAIAPLPAPEVRGRVLPDNLAYVIFTSGSTGVPKGAMLNHRGMLNHLLAKVE
                     ELEITAADVVAQNASQSFDISVWQLLAALAVGGRVHIILDDVAHEPARLLAEADAAGV
                     SILEVVPSVLGAMLAGEGGAAPLRSLRWMIPTGEALPPELARRWLDAYPQVPLLNAYG
                     PTECSDDVSHHPLRDVPEGAMQIPIGRPVGNTRLYVLDRGLRPLPAGVAGELCVGGAG
                     VGRGYLLEPARTADVFVPDPFAGLTGEEPGSRLYRTGDLARLLPDGTLEFLGRIDHQV
                     KVRGFRIELGEIEAVLCEHPALRQAVVSALDGGAAGKRLVAYVVLRTDDGIIAPDAAE
                     LRRFIADRLPDYMVPSAFVVLPDIPLTAHGKVDRRALPVPELARMEGDEPFVAPRNEV
                     EEAVAEIWKEILKVERVGAFDSFFALGGHSLVAAQVAAKIRAAFEVELPLRTLFEAPT
                     LGELALAVEELMIARIESLSEEELAELL"
     gene            3350..>6999
                     /locus_tag="VEL74_20125"
     CDS             3350..>6999
                     /locus_tag="VEL74_20125"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64900.1"
                     /translation="MTEPVSVMAPPAAGPLDRPGLIHRFFEEQVRRTPERVALLFAPG
                     PGAPRGRMTYAGLNRRANRLAYRLRGLGVGPESRVAVALERSPDLVVTLLAVLKAGGA
                     YVPLDPAYPRRRLALILEGSQAGVSAPVLVTRERLLRDLPEPVPGTRTVLLDRAEPSE
                     VREEDSAAGVLPENLAYVLYTSGSTGVPKGVAVTHRSAAALLRWARETYSPEELAGVF
                     ASTSINFDLSVFELFVPLSWGGRVILGENALALATTPAAGDVTLINTVPSAVTELLRM
                     GAVPPSVRTVNLAGEPLRNALAQRLYGLGTVERVWNLYGPTEDTTYSTGVLVEPGSAG
                     EPSIGWPIPGTRARVLEVTEDGGMRPVPPASNARDEPGELFLAGEGLARGYLHRPDLT
                     AERFLPDPLATEAGGRMYRTGDLVRQYPSGELEFLGRADHQVKIRGFRIELGEIEAAL
                     AAHPAVRLVSVLPWLDAAGDKRLAAYVEAGTAAPPSTGELLDFLRARLPEFMLPSAFV
                     FLPALPLTPNGKVDRQVLAAMSPEEAGERVVGLVAPRTAAERTMAEIWAEALGLGQVG
                     LRDKFLELGGHSLLATRVLSRVRDVFGVELPPRTLFTAPTVEALAARVQASVAEERRL
                     QAPPILPVPRPEQIPPSFAQERLWFLDRLEPGQASYHLPTAVRLRGRLAVLALEWGLN
                     ELARRHEVLRTAFPEGSEGPYQAIAPVTPLPLPVVDLAALPEGARATEAARRLEAEAQ
                     RPFDLAAGPLLRTVLFRVDGGESVLLINTHHIVSDGWSQVVLVRELAALYEAFSRGLS
                     SPLPELPVQYADFALWQRDWLSGEVFASQVEYWKQELEGAPARLELPADRRRAPDHVQ
                     GKRRPLALSREITEGLHALSRSQGATLFMALLAGYQALLYRLTGARDLLVGVPIANRN
                     HSEIEGLVGFFVNTLVLRGQPTPELPFRHLLNRAAAVAFGAYAHQDLPFERLVEEMRA
                     ERSPGVLPLIQTMLTLQNLPPGPRPLPELELDWLDLQLVPSELDLGIVLTEAGGELTG
                     SVEYRTDLFDAATASRFAGHLAVLLAGAVADPDRRLSDLPLLTPAERRQLIEWNETGL
                     VAASLPVHRRFEQQARRTPDASAVISGSDRLTYAELDHRATLRARHLRSLGAGPETRV
                     GLLLDRGVDLAVGILAIWKAGAAYVPLDPSQPDARLALIAADAFRDQASPILVVNDHL
                     RQRALDLFQGEG"
BASE COUNT          856 a         2540 c         2548 g         1055 t
ORIGIN      
        1 cggcggcgga gctggcggag gcgatcgcct tcatggtgca ggaggccgca ggggggagcg
       61 ccgagggtgt cgcggcggtc cccgcgattg gaccgagccc gttgcgggcg gcgggcgggc
      121 cgttcccgct ctccttcgcc caggagcgga tctggttcct cgaccagctc gagcccggca
      181 ctgcggcctt cgacctgccg gccgccaccc ggctccaggg acgcctcgat cgcgccgccc
      241 tggagcacag cctgaacgag gtggtgcggc ggcacgagac cctgcgcacc cgcttcctga
      301 cggtggacgg gcaggtggtg caggaggtgc tgccatcttt ggatctggat cttcccatgg
      361 ccgatctctc ggcgctgccg ggggagagcc gcgcggccga ggagcggcgg ctcgtgctcg
      421 accacgcctc gcgccccttc gatctggcga acgggccgct cgccgccttc ctgctgctgc
      481 gtctcacgcc ggaggaccac ctgctggctc tggtgatgca ccacatcatc tccgacggct
      541 ggtcgctggg cgtgctgcag agcgaggtgg cgcggcacta cggcagggct ctcctgcccg
      601 tctccgagcc gctgccggag ctgtcgttcc actacgccga ctacgccgac tggcagcgcc
      661 gccacttcca gggggacgtg ctggagcgtg agctggccta ctggcgcccc cggctccacg
      721 gggtgccgtt cctggagctg cgcggcgacc gcccccggcc gccggtccag accttccgcg
      781 gcggctacga ggacttcacc ctgccggccg ggctcgcccg ctccctggag gggctggcac
      841 agcgcgaggg ctcctccctc ttcatggtcc tgctgaccgg cttcgacctg ctgctccagc
      901 gccacgcgca gcaggacgac tttgccgtcg gcgccctgat cgccggccgc aaccgcgcgg
      961 agctggaggg gatgatcggc tgcttcgtca acaacctggc gctacgcacc gacctctccg
     1021 gtgagccgga cggccgggag ctgctgcgcc gggtgcgcga gaccacgatc gaggcctacg
     1081 cgcaccagga cgtgccgttc gaaaagctcc tcgaagagct ccagccggag cgcgacctgt
     1141 cgcgcggcgc gctgatccag gtgatgttca acctgctcaa cttccccacg gtgcacgagg
     1201 agctgccggg gctcaccatg agcagctccg gcgtgcgcgg cgagcgggcc aacttcgacc
     1261 tcaccctctg gatgaccgag gcacgggacg gcctggtggg atggctggag tacaacgccg
     1321 acctcttcga ccgcgccacg gcccggcgca tgaccgccca cctgcggcgg ctgctggagg
     1381 ggctggcggc cgatcccggc cagcgggtgg cggagctgcc gctgctcggc gaggccgagc
     1441 gccggcaggt gctggtggag tggaatcgca ccggccgcga gcgcccctgg gaggagggct
     1501 tcctcggcct cttccaggcg cgggtggaga gcgtgccggg cgatgtcgcc gccgtctgcc
     1561 agggggagag cctcacctac gccgagctgg gccgccgctc ccgccgtctc gccgcatggc
     1621 ttgccagcga aggcgtcggc ccggagtcgg tggtgccgat cctggccgac cgcggcctgg
     1681 acttcctggc cgcggttctg ggcgtgctcc aggccggtgg cgcctatctg cccctcgacc
     1741 cgaatcagcc ggcgtcgcgg gccgtgcggg tgctgcgcca gagcggcgcg gcgctggtgc
     1801 tggtggggag cgatcacgct ctccgcctgg tcgcggcctc cgagggcggg gcgctgccgg
     1861 acatcgtggc cttcgagggc ctgctccggg acgaggcgat cgctccgctg ccggctcccg
     1921 aggtccgggg cagggtgctg ccggacaacc tggcctacgt gatcttcacc tccggctcca
     1981 ccggcgtgcc caagggggcg atgctcaacc accgcggcat gctcaaccac ctgctggcca
     2041 aggtggagga gctggagatc acggcggcgg acgtggtggc gcagaacgct tcgcagagct
     2101 tcgacatctc ggtctggcag ctcctggcgg cactggccgt gggcggacgg gtgcacatca
     2161 ttctggatga cgtggcccac gagccggcgc gcctgctggc cgaggccgac gccgccggag
     2221 tctccatcct ggaggtggtg ccgtccgtgc tgggagccat gctggccggc gaaggcgggg
     2281 cggcgcctct gcgctcgttg cgctggatga tccccaccgg cgaggcgctg ccgcccgagc
     2341 tcgcccgccg ctggctcgac gcctatccgc aggtgccgct gctcaacgcc tacggtccga
     2401 cggagtgctc ggacgacgtc agccatcacc cgctccgcga cgtacccgaa ggggccatgc
     2461 agatcccgat cggccggccg gtgggcaata cccgcctgta cgtcctcgac cgcggcctgc
     2521 ggccgctgcc ggcgggcgtg gccggggagc tgtgcgtggg cggcgccggc gtgggccgcg
     2581 gctacctcct ggagcccgcg cggaccgccg acgtcttcgt gcccgacccg ttcgccggcc
     2641 tgacgggaga ggagcccggg agccgtctct accggaccgg cgacctcgcc cggctgctgc
     2701 ctgacgggac cctggagttc ctggggcgga tcgatcacca ggtcaaggtg cgcggcttcc
     2761 gcatcgagct gggggagatc gaggcggtgc tgtgcgagca cccggcgctg cggcaggccg
     2821 tggtgtcggc gctcgacggt ggtgccgccg gcaagcgact ggtggcctac gtggtgctgc
     2881 gcaccgacga cggcatcatt gcccctgacg cggcggagct gcgccgcttc atcgccgaca
     2941 ggctccccga ctacatggtg ccgtcggcct tcgtggtcct gccggatatc ccgctgaccg
     3001 cccacggcaa ggtcgaccgc cgcgccctgc cggtccccga gctggcacgg atggaggggg
     3061 acgagccgtt cgtggcgccg cgcaacgagg tggaggaggc ggtagccgag atctggaagg
     3121 agatcctcaa ggtcgagcgg gtcggcgcct tcgacagctt cttcgcgctg ggcgggcact
     3181 ctctggtcgc cgcgcaggtc gcagccaaga tccgcgccgc cttcgaggtg gagctgccgc
     3241 ttcgcaccct gttcgaggcc cccactctgg gcgagctggc tctcgcggtc gaggagctga
     3301 tgatcgctag gatcgagagt ctgagcgagg aagagctggc ggagctgcta tgaccgagcc
     3361 ggtgtcggtg atggcgccgc cagcggccgg gccgttggac cggccggggt tgatccatcg
     3421 cttcttcgag gagcaggtcc ggcgcactcc cgagcgggtg gcgctgctct tcgcccccgg
     3481 ccccggcgcg ccacggggcc ggatgaccta cgccggcctg aaccgccgcg ccaaccgctt
     3541 ggcgtaccgg ctgcgtgggc tcggtgtcgg ccccgagtcg cgcgtcgccg tggcgctgga
     3601 gcgctcgccc gatctggtcg tcaccctgct ggcggtcctc aaggcgggcg gcgcctacgt
     3661 gccgctcgac ccggcgtacc cgcgccggcg gctggcgttg atcctagaag gctcccaggc
     3721 cggggtcagt gcgcctgtgc tggtgactcg cgagcgcctg ctccgcgacc tgcccgagcc
     3781 ggtgcccggc acccggacgg tgctcctcga ccgtgccgag ccctccgagg tgagggagga
     3841 ggattcagcc gctggcgtgc tgccggagaa cctggcctac gtcctctaca cctccggctc
     3901 caccggtgtc cccaaagggg tggcggtgac ccaccgcagc gccgcggcgc tgctgcgctg
     3961 ggcgcgggag acctactcgc cggaggagct ggccggcgtc ttcgcctcga cctccatcaa
     4021 cttcgatctc tcggtctttg agctgttcgt ccccctctcg tgggggggga gggtgatcct
     4081 gggggagaac gccctcgctc ttgcgaccac ccccgcggcc ggcgacgtga cgctgatcaa
     4141 caccgtgccc tcggcggtta ccgagctgct gcgcatggga gcggtgccgc cgtcggtgcg
     4201 caccgtcaat ctggcgggcg agccgctgcg caacgccctg gcccagcgcc tctacggcct
     4261 cgggaccgtg gagcgggtct ggaacctcta cggcccgacg gaggacacca cctactcgac
     4321 cggcgtcctg gtcgagccgg ggagcgccgg ggagccgtcg atcggctggc cgatccccgg
     4381 gacccgtgcc cgcgtgctgg aggtgacgga ggacggtggc atgcggccgg tgccgccggc
     4441 gtccaacgcg cgggacgagc cgggcgagct gttcctggcg ggggaggggc tggcccgcgg
     4501 ctacctccac cggcccgacc tgacggccga gcgcttcctc cccgacccgc tggcgaccga
     4561 ggcggggggg cggatgtacc gtacgggcga cctggtccgg caatatccgt cgggagagct
     4621 cgagttcctg ggccgcgccg accatcaggt caagatccgc ggcttccgca tcgagctcgg
     4681 agagatcgag gcggcgctcg ccgcccatcc ggcggtccgc ctggtctcgg tgctgccctg
     4741 gctggatgcc gccggcgaca agcgcctggc ggcctacgtg gaggccggga cggcggcgcc
     4801 accgtccacg ggcgagctgc tggatttcct gcgcgcccgc ttgcccgagt tcatgctgcc
     4861 gtcggccttc gtgttcctgc cggcgctccc actgacgccc aacggcaagg tggaccgcca
     4921 ggtgctggcg gccatgagcc cggaggaggc gggggagcgg gtcgtggggc tggtggcgcc
     4981 gcgtaccgcg gctgagcgca ccatggcgga gatctgggcc gaagcactgg gcctcggcca
     5041 agtcggcctg cgcgacaaat tcctggagct gggcggccac tcgctgctcg ccacccgtgt
     5101 cctctcccgg gtgcgcgacg tgttcggcgt ggagctgccg ccacggacgt tgttcaccgc
     5161 tcccaccgtg gaagccctag cggcgcgggt ccaggcgtct gtggccgagg agcggcggct
     5221 tcaggcgccg ccaatcctgc cggtgccgcg gccggagcag atccccccct ccttcgccca
     5281 ggagcggctc tggttcctcg accggctgga gccgggccag gcgtcgtacc acctgccgac
     5341 ggcggtgcgc ctgcgcggcc gtctcgccgt actggcgctg gagtggggcc tgaacgagct
     5401 ggcgcgccgg cacgaggttc tgcggaccgc ctttcccgaa ggaagcgagg ggccgtatca
     5461 ggcgatcgcg cccgtcacgc ccctgccgct gcccgtcgtc gacctcgcgg cactgccgga
     5521 gggtgccagg gcgaccgagg ccgcccggcg gttggaggcc gaggctcagc gtcccttcga
     5581 cctggccgcc ggaccactcc tgcggaccgt actcttccgt gtcgatggcg gggagagcgt
     5641 gctgctgatc aacacgcacc acatcgtgag tgacggctgg tcgcaggtgg tgctggtacg
     5701 cgagctcgcg gcactgtatg aggccttctc ccgcggtctc tcctcgcccc tgccagagct
     5761 gccggtgcag tacgccgact tcgccctctg gcagcgcgac tggctgtcgg gcgaggtgtt
     5821 cgcgtcgcag gtggagtact ggaagcagga gctggaaggc gcgccggcgc ggctggagct
     5881 gccggcggac cggcggcggg cccccgacca tgtccaaggg aagcgccggc cgctggcact
     5941 gtcgcgggag atcacggagg ggttgcacgc cctctcccgc agccagggcg ccaccctctt
     6001 catggccctg ctggccggtt accaggcgct gctataccgc ctgacgggcg cgcgggacct
     6061 gctggtgggc gtgcccatcg ccaaccgcaa ccacagcgag atcgagggcc tggtcggctt
     6121 cttcgtcaac accctggtgc tgcgcggcca gcccactccg gagctgccgt tccgccacct
     6181 gctgaaccgc gccgcggctg tggcgttcgg cgcctatgcc caccaggact tgccgttcga
     6241 gcggctggtg gaggagatgc gggccgagcg cagccccggc gtcctccccc tgatccagac
     6301 catgctgacg ctccagaacc tgccgccagg gccgcgccct ttgccggagc tggagctcga
     6361 ctggctggac cttcagctcg tgccctcgga gctcgacctg gggatcgtgc tgaccgaagc
     6421 cggcggcgag ctgaccggct cggtggagta ccgcaccgat ctcttcgacg ccgccaccgc
     6481 ctcgcgtttc gccggacatc tggcggtcct gctggcgggg gccgtggccg accccgaccg
     6541 gcgcctttcc gacctgccgc tgctcacccc ggccgagcgc cgccagctca tcgagtggaa
     6601 cgagaccggt ctcgtcgcgg cttccctccc ggtccaccgc cgcttcgagc agcaggcccg
     6661 gcgcactccc gacgcttcgg cggtaatctc cggctccgac cgcctgacct atgccgagct
     6721 ggaccaccgc gccaccctgc gggcccgaca cctgaggagc ctgggagcgg gccccgaaac
     6781 gcgcgtcggc ctccttctcg accgcggcgt cgacctggcc gtgggcatcc tcgccatctg
     6841 gaaggcggga gcggcctacg tcccgctcga cccgtcgcag ccggacgccc gcctagccct
     6901 gatcgccgcg gacgccttcc gcgatcaggc ctcccccatc ctcgtcgtca acgaccacct
     6961 ccggcagcgc gccctcgatc tcttccaggg ggaggggga
//
LOCUS       DASPJI010000718         8323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_102822, whole genome shotgun sequence.
ACCESSION   DASPJI010000718 DASPJI010000000
VERSION     DASPJI010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102822"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /gene="dprA"
                     /locus_tag="VEL74_20130"
     CDS             complement(<1..884)
                     /gene="dprA"
                     /locus_tag="VEL74_20130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HYG64901.1"
                     /translation="MKALPTGRELLIALNAAPSLSRPALYRLAAELERWAGPRQPEDT
                     AESLSSKVGVPVLQMTRALAALPGAADLAARETAEADRLGAAIVTTLDPGYPPPLRQL
                     APPPPVLYIEGRLSAGSAEPAVAVVGSRRADPYGREVAGLFAQALAAAGVPVVSGFAL
                     GVDEAAHRGALAAERGETVAVLGCGLGVDYPRGQGALAREIAGRGAVVSEFPCGQEPR
                     AWHFPVRNRVIAALTAGTLVVQAAPRSGSLITARHALDLGREVWAVPGRIFDEKSIGP
                     NALIRDGAQLVQHPRDIL"
     gene            1071..1766
                     /locus_tag="VEL74_20135"
     CDS             1071..1766
                     /locus_tag="VEL74_20135"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG64902.1"
                     /translation="MTLFHDSNVPPSAPAARRPRIPRPRAAALLLPVVVAALLLPACS
                     GNRNPGRSEDQLRFGVDMARRGLWSEALFRFQEAQRLDPQSPRILNNLAVASEALGQF
                     DTALEYYRRALQLDPNSREARANYSRFAEFYQSFRGPQRGAPRPGAPAPAGTPGSAPD
                     TAPGAAPGEPAPADVPEPLPSGPVTPPGQAPIEPPTPPTGNVATPPNNEPPSPSPPPP
                     ASSPAGSGLQAFR"
     gene            1854..2567
                     /locus_tag="VEL74_20140"
     CDS             1854..2567
                     /locus_tag="VEL74_20140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64903.1"
                     /translation="MKRNRSWTRTTLLLLLAALPALTAARPAGAAVEVRLKLPQRARL
                     DLAGRKTLAVSPFIVISQEGDERIQGRNIDVQKEFARYLLKLLRRDTELKIQESGPVD
                     FPVFDLEQLAKQQDFWAAMGERLGADLILTGGLDFDVQDRTGYKTEEFVSPYNGRTYY
                     RQVLVEQTGFQYDILMQVYDGRTGALLYQDNFKDFQSFEGEADPLAGMFENLMSLEDR
                     ILNVFTQKEVEATRVLYTD"
     gene            2586..5513
                     /locus_tag="VEL74_20145"
     CDS             2586..5513
                     /locus_tag="VEL74_20145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64904.1"
                     /translation="MTRQPRCLLAGGLILLGSLVPLQPLAAQYGSGPQWNGQGFGKNK
                     VQYRDFNWSIYHSPHFNVYYYPSEEPLLQKVVSYAESAYDQLSREFNFQIQEPVPLIF
                     YATHSAFEQNNIILNFIPEGTGAFATPTRFRMVLPVDLPDDQLMQLILHELTHIFQYH
                     LLFQGSLAKAVTSAPPQWFMEGMASYMAKDEEAREYMYLRDGVVNDRIPSVTKGAGPQ
                     GFFAYRLGHAVFDFVEERWGKEGFLDFIYEIRNSLGFQVDRAVERAFKMEGEDFDAEF
                     RRWLRRKYLPELVQTGEPGDFGRIFRIRDEPSGTEISPTASPSGDLVASFSYYQGDLD
                     VVLFDTEKRTLIRNLTKGYSNQYQYLVAQELALGRAMGRDLAFSPDGNTIAVFAKREQ
                     GRSLVLLDVLNGGMRRIIDDMGGIEQQHAPSWSPDGRRIAFYGNRNGQFAIFVYDLES
                     GQITNLTNDAVYDGAPTFSPDGKSIAFLSVVGQGFAKLFRMDLAQPGQRYQLTTGDSN
                     ENDPIYSPDGQRIYYTSDRNGLQNIYSLDLTSGEVRQYTNVVTGAFMPTVLREPLGEE
                     RLVYTGFWKGDFGLYVTDVDEPVTEPTRTELPVQPTQATELPVFEPDIQVTLDDANKE
                     RYGGFKFFLEDAATTVAIDDDQNYLGQVVLTFSDYLGDRRIIGAFSSIESFSNFDVVY
                     ADLGQRLQWQVHLFDDRSFFIGEEFVGTGRIERGRAYFKQTGLVGSLVYPFSFYARAE
                     LGAGYLLREFLAQTEGIDPETGELVPVLVPIDDNFPIIQGALVGDSTRFTSWGPVAGR
                     RWRLDTSYAPDLEESGTLFWSSDLDFRQYIQVTQRSNLALRGFFGYAEGNQPNPYYFG
                     GLDTLRGTDFRDLSGDRAFFANIEYRFPLIDVVATPIVVFQGVRGVLFFDVGGAWYDG
                     FEDFDFYDSEEGRLEDAIAAYGWGVTIRLFGLDLNWDMAKRYDFDTSEDGFRTSFWIG
                     TRF"
     gene            5693..6400
                     /locus_tag="VEL74_20150"
     CDS             5693..6400
                     /locus_tag="VEL74_20150"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HYG64905.1"
                     /translation="MTCLLVNGTVALDAGSLSQVLPIEQQVEVRSILLTHSHMDHTNS
                     LPFFIENVFGRGEGIDIHASSPTIYAIRKYLFNNATWPDFTRLPNHLLPSVRFQELES
                     EVPLVLDGVSFTPIPVEHLVPTHGFLIEENGAAVLWSSDTGPTQRFWEVANRTPNLKA
                     VCIETSFDNSLQPIADVSLHLTPRTLVEELRKLEKKVPVFIHHLKPPCTARIREELRQ
                     LKNPDLQLLEQGKEYRF"
     gene            6474..7403
                     /locus_tag="VEL74_20155"
     CDS             6474..7403
                     /locus_tag="VEL74_20155"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYG64906.1"
                     /translation="MLPSLHKLSRKRRTADECAQAAFDFGPAPEPAKPVEAPAPKATV
                     PAGAPPLRVGVLGSGSGGNAMVVECGSRRILVDAGFSAREIKRRLAFFEVEPESLEAL
                     VLTHEHGDHCRGADRLAKKYGLPVLATAGTLRETRLGEQATRQAEVIRSGEPREVAGF
                     RIEPFVIPHDAREPVGFLIEDPYGRRVGLAADLGCRTQLAWGRLADLDVLVLETNHDL
                     DMLRTGPYPWSLKQRVAGRHGHLSNREAAEGLPELICDRLRWVVLYHLSRTNNLPALA
                     AAAVGEVLDRERCPAQMAVTEQDHPTPWLEVNA"
     gene            7400..7660
                     /gene="purS"
                     /locus_tag="VEL74_20160"
     CDS             7400..7660
                     /gene="purS"
                     /locus_tag="VEL74_20160"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HYG64907.1"
                     /translation="MKARVTVYPRREILDPQGKAIRDALSRVGFVGVDDVRAGKSFEI
                     SLGTDDPGEADRQLREMCEKLLANTVVEDYSVELLRGEGARS"
     gene            7657..>8323
                     /gene="purQ"
                     /locus_tag="VEL74_20165"
     CDS             7657..>8323
                     /gene="purQ"
                     /locus_tag="VEL74_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HYG64908.1"
                     /translation="MKCGVVVFPGSNCDHDVYHVLKHVLEQDTTFLWHQDDTLKGCDL
                     VVLPGGFSYGDYLRAGALAALSPVMGAVRRHAEAGGLVLGICNGFQVLLETGLLPGAM
                     RRNANLRFQCRDVLLKVERNDLPFTRHYEPGQVIRIPIAHAEGNYEDTEERLDALEAA
                     GQVVFRYVTPDGIPGAPGSEWNPNGAARAIAGVCNPQGNVLGLMPHPERCSEPILGGT
                     DGLA"
BASE COUNT         1287 a         3077 c         2722 g         1237 t
ORIGIN      
        1 tccaggatgt cccgcgggtg ctggacgagc tgggcgccgt cgcggatcag ggcgttgggg
       61 ccgatcgact tctcgtcgaa gatgcgcccc ggcacggccc agacctcccg gcccaggtcc
      121 agggcgtggc gcgcggtgat cagcgacccg gagcgcggcg ccgcctggac gaccagggtc
      181 cccgcggtga gcgcggcgat cacccggttg cggaccggga agtgccaggc ccgcggctcc
      241 tggccgcagg ggaactccga gaccacggcg ccgcggccgg cgatctcgcg ggcgagcgcc
      301 ccctggccgc gcgggtagtc gacgcccagg ccgcagccga ggacggcgac cgtctccccc
      361 cgctccgccg ccagggctcc ccggtgcgcc gcctcgtcga cgcccagcgc gaagccggag
      421 accaccggga cgccggccgc cgccagggcc tgggcgaaga ggccggccac ctcgcggccg
      481 taggggtcgg cgcgccgcga gcccaccacc gccaccgccg gttccgccga ccctgcagat
      541 aaccgcccct ctatataaag gacggggggc ggcggcgcga gctggcgcag cggcggcggg
      601 tagccggggt cgagggtggt gacgatggcg gcccccaggc ggtccgcctc ggcggtctcg
      661 cgcgcggcga ggtccgcggc gccggggagc gcggcgaggg cgcgggtcat ctggaggacg
      721 ggcacgccca ctttggagga caggctctcc gccgtgtcct ccggctggcg ggggccggcc
      781 cagcgctcca gctcggcggc cagacggtag agcgcggggc gcgacaagga gggggcggcg
      841 ttcagggcga tgagcagctc gcggccggtc ggtaaagctt tcatgcgaag gttgtctccc
      901 tgggaaggtg gttcaacccg ggaaagacgg aatcggcggg aaaaatcgct caaaaaaatc
      961 cgccggccgg cgggcggcgt acttgcatgc agagggggat gatggaacct ttctccgggc
     1021 cccggcggcc tgaccccttg tgtatactag gcaccttgga acattgggcg atgacgctct
     1081 ttcacgactc gaacgtccct ccgtccgccc cggccgcgcg ccggccgcgg attccccggc
     1141 cccgggccgc cgcccttctc ctgccggtgg tcgtcgcggc cctcctcctc cccgcctgca
     1201 gcggcaaccg caaccccggc cgttcggagg atcagctccg gttcggcgtc gacatggcgc
     1261 gccgcggcct gtggagcgag gcgctcttcc gtttccagga ggcgcagcgg ctcgacccgc
     1321 agagcccccg gatcctcaac aacctggcgg tggcctccga ggcgctgggc cagttcgaca
     1381 cggcgctcga gtactaccgg cgggccctgc agctcgaccc gaacagccgt gaggcgcggg
     1441 ccaactattc gcgcttcgcc gagttctacc agagcttccg ggggccgcag cgcggggctc
     1501 cccggcccgg cgcgccggct ccggccggca ctccaggcag tgccccggac acggcccctg
     1561 gagccgcccc cggcgagccc gcgccggcgg acgttccgga gccgctcccc agcgggcccg
     1621 tgacgccgcc ggggcaggcc ccgatcgagc cgcccacgcc gccgaccggc aacgtggcca
     1681 ctccgcccaa caatgaacca ccgtcgcctt cccccccgcc gccggcatcg tcgccggcgg
     1741 ggtcaggcct tcaggcattc cgctgagcgc ggccaggcgc cgccgcccct ctccggaggg
     1801 gccggtgcct cccgccttcg gcgtggcagt cagtcttcac aaggaggttt gacgtgaagc
     1861 gcaaccgttc ctggacccgg accacccttc tcctgctgct cgccgccctg cctgcgctga
     1921 ccgccgccag gcccgccggc gcggcggtgg aggtccgcct caagctgccg cagcgcgccc
     1981 ggctcgacct ggccggccgc aagaccctcg ccgtctctcc cttcatcgtc atcagccagg
     2041 agggggacga gcggatccag ggccgcaaca tcgacgtgca gaaggagttc gcccgctacc
     2101 tgctcaagct gctgcggcgc gacaccgagc tcaagatcca ggagtccggc ccggtcgact
     2161 tcccggtctt cgacctggag cagctcgcca agcagcagga cttctgggcc gccatgggcg
     2221 aacgcctcgg cgccgacctg atcctcaccg gcggcctgga cttcgacgtc caggaccgca
     2281 cgggctacaa gacggaggag ttcgtctccc cgtacaacgg ccgcacctac taccggcagg
     2341 tgctcgtcga gcagaccggc ttccagtacg acatcctgat gcaggtctac gacgggcgca
     2401 ccggcgctct gctctaccag gacaacttca aggacttcca gagcttcgaa ggggaggcgg
     2461 acccgctcgc cggcatgttc gagaacctga tgtccctgga agaccggatc ctcaacgtct
     2521 tcacccagaa agaggtggag gcgacgcgcg tcctgtacac cgactgaggt gcaaaggaga
     2581 tctctttgac ccgtcaacca cgatgccttc tcgccggcgg gctgatccta ctcggctcgc
     2641 tggtcccgct ccagccgctc gcggcccagt acggctccgg cccgcaatgg aacggccagg
     2701 ggttcggcaa gaacaaggtc cagtaccgag acttcaactg gagcatctac cactcgccgc
     2761 acttcaacgt ctactactat ccttccgagg agccactgct ccagaaggtg gtctcgtacg
     2821 ccgagagcgc ctacgaccag ctctcccggg agttcaactt ccagatccag gagccggtgc
     2881 cgctcatctt ctacgccacg cactcggcgt tcgagcagaa caacatcatc ctcaacttca
     2941 tccccgaggg caccggcgcg ttcgccacgc ccacccgctt ccgcatggtg ctgccggtcg
     3001 acctgccgga cgaccagctc atgcagctca tcctccatga gctgacccac atcttccagt
     3061 accacctgct gttccagggg agcctggcca aggcggtcac ctccgcgccg ccgcagtggt
     3121 tcatggaagg catggcgagc tacatggcca aggacgagga ggcccgcgag tacatgtacc
     3181 tgcgggacgg cgtggtcaac gaccgcatcc cgtcggtcac caagggggcc gggccgcagg
     3241 ggttcttcgc ctaccggctg ggccacgcgg tcttcgactt cgtcgaggag cggtggggca
     3301 aggaggggtt cctcgacttc atctacgaga tccgcaactc cctcggcttc caggtggacc
     3361 gggcggtgga gcgggccttc aagatggagg gggaggactt cgacgccgag ttccggcgct
     3421 ggctgcgccg caagtacctg ccggagctgg tgcagaccgg cgagcccggg gacttcggcc
     3481 gcatcttccg gatccgcgac gagccctccg gcaccgagat ctcgcccacc gcgtcgccgt
     3541 cgggcgacct ggtggcctcc ttctcgtact accagggcga cctcgacgtc gtgctgttcg
     3601 acaccgagaa gcggaccctg atccgcaacc tcaccaaggg ctactccaac cagtaccagt
     3661 acctggtcgc ccaggagctg gcgctgggcc gggcgatggg gcgcgacctc gccttctcgc
     3721 ccgacggcaa caccatcgcc gtcttcgcca agcgcgagca ggggcggagc ctcgtgctgc
     3781 tcgacgtgct caacggcggc atgcggcgga tcatcgacga catggggggc atcgagcagc
     3841 agcacgcccc ctcctggagc cccgacggcc gccgcatcgc cttctacggc aaccggaacg
     3901 gccagttcgc catcttcgtc tacgacctcg agagcggcca gatcaccaac ctgaccaacg
     3961 acgcggtcta tgacggcgcc cccaccttct cgccggacgg caagtccatc gccttcctct
     4021 ccgtggtagg ccagggcttc gccaagctgt tccgcatgga cctcgcccag ccgggccagc
     4081 gctaccagct caccaccggc gactccaacg agaacgaccc catctactcg ccggacggcc
     4141 agcggatcta ctacacgtcc gaccgcaacg gcctgcagaa catctacagc ctggacctga
     4201 cgagcggcga ggtccgccag tacaccaacg tggtgaccgg cgccttcatg ccgaccgtcc
     4261 tgcgcgagcc gctgggcgag gagcgcctgg tctacaccgg cttctggaag ggggacttcg
     4321 gcctctacgt gaccgacgtc gacgagccgg tcaccgagcc gacgcggacg gagctgccgg
     4381 tccagcctac ccaggccacc gagctgccgg tcttcgagcc ggacatccag gtgaccctgg
     4441 acgacgccaa caaggagcgg tacggcggct tcaagttctt cctcgaggac gccgccacca
     4501 ccgtcgccat cgacgacgac cagaactatc tgggccaggt ggtcctcacc ttctcggact
     4561 acctgggcga ccggcggatc atcggcgcgt tcagctcgat cgagagcttc tccaacttcg
     4621 acgtggtcta cgccgacctc ggccagcggc tgcagtggca ggtgcacctg ttcgacgacc
     4681 gaagcttctt catcggcgag gagttcgtcg gcaccggccg gatcgagcgc ggccgggcgt
     4741 acttcaagca gaccggcctc gtcggctccc tcgtctatcc gttctccttc tacgcgcggg
     4801 cggagctggg agcgggctac ctgctgcgcg agttcctggc gcagaccgag ggcatcgatc
     4861 cggagaccgg tgagctcgtt ccggtcctgg tccccatcga cgacaacttc ccgatcatcc
     4921 agggggccct ggtgggcgac tccacccggt tcacctcctg ggggccggtc gccgggcggc
     4981 ggtggcggct ggacacctcc tacgcccccg acctcgagga gagcggcacg ctcttctgga
     5041 gctcggacct cgacttccgg cagtacatcc aggtgaccca acgcagcaac ctggcgctcc
     5101 ggggcttctt cgggtatgcc gaaggcaacc agccgaaccc gtactacttc ggcggcctcg
     5161 acaccctgcg gggcacggac ttccgggacc tgtcgggtga ccgcgccttc ttcgccaata
     5221 tcgagtaccg gttcccgctc atcgacgtgg tggcgacgcc catcgtcgtc ttccaggggg
     5281 tgcgcggcgt cctcttcttc gacgtcggcg gcgcctggta cgacggcttc gaggatttcg
     5341 acttctacga ctcggaggag gggcgtctcg aggacgccat cgcggcctac ggctggggtg
     5401 tcaccatccg gctcttcggc ctcgacctca actgggacat ggccaagcgg tacgacttcg
     5461 acacgtcgga ggacggcttc cgcacctcct tctggatcgg cacgcgcttc tgacgtcccg
     5521 gtccatccca ccctgatccc accttgaaga gagaaggggg gcctcacggc cccccttttt
     5581 cttggcgcgc cgcgggtttc ttcccctttg accaagagca gggatacaat cctcgctata
     5641 tatgcggatt caggttctcg ggagctatgg gggcgaaagc ccggattgcc gcatgacctg
     5701 cctgctggtc aacggcacgg tcgcgctcga cgcggggtcg ctgtcgcagg tgctgccgat
     5761 cgagcagcag gtggaggtgc gctcgatcct cctcacccac tcccacatgg accacaccaa
     5821 ctcgctgccg ttcttcatcg agaacgtctt cggccgcggc gaggggatcg acatccacgc
     5881 ctcgtcgccg acgatctacg ccatccgcaa gtacctgttc aacaacgcca cctggccgga
     5941 cttcacccgc ctgcccaacc acctgctgcc gtcggtccgc ttccaggagc tcgagagcga
     6001 ggtgccgctg gtcctcgacg gcgtctcctt cacgccgatc ccggtggagc acctggtgcc
     6061 gacccacggc ttcctcatcg aggagaacgg cgcggcggtc ctctggtcga gcgacaccgg
     6121 gccgacccag cgcttctggg aggtggcgaa ccgcaccccc aacctcaagg cggtctgcat
     6181 cgagaccagc ttcgacaact ccctccagcc gatcgccgac gtgtcgctgc acctcacccc
     6241 gcggacgctg gtggaggagc tgcgcaagct ggagaagaag gtgccggtct tcatccatca
     6301 tctgaagccc ccctgcaccg cccgcatccg cgaagagctc cgccagctca agaaccccga
     6361 cctgcagctc ctcgaacagg gcaaggaata ccggttctga ggggattctg acgagatagg
     6421 ggcgcacacc caacgggcag ggtgtggcga ctccggctat actacccctt gcagtgctcc
     6481 cctccctcca caagctctcc cggaagcgtc gcaccgcgga cgagtgtgcg caggcggcgt
     6541 tcgatttcgg ccccgcgccg gagcccgcca agccggtcga ggcccctgcg ccgaaggcga
     6601 cggtgcccgc cggggctcct cccctgcgcg tcggcgtcct ggggtcgggg agcggcggca
     6661 acgccatggt ggtggagtgc ggatcgcggc gcatcctcgt cgacgcgggc ttctccgcgc
     6721 gggagatcaa gcgccgcctg gccttcttcg aggtggagcc ggagagcctc gaggccctgg
     6781 tcctcaccca cgagcacggc gaccactgcc gcggcgccga ccggctggcc aagaagtacg
     6841 ggctgccggt cctcgccacc gccgggaccc tgcgcgagac gcggctcggc gagcaggcga
     6901 cgcgccaggc cgaggtgatc cgctccggcg agccgcgcga ggtggccggc ttccgcatcg
     6961 agccgttcgt catcccgcac gacgcccggg agccggtcgg cttcctgatc gaggacccct
     7021 acggccgccg cgtcgggctg gcggccgacc tcggctgccg cacccagctc gcctgggggc
     7081 ggctcgccga cctcgacgtc ctggtgctcg agaccaacca cgacctcgac atgctgcgca
     7141 ccggcccgta tccttggagc ctgaagcagc gggtcgccgg gcgccacggc cacctctcga
     7201 accgggaggc ggccgaaggg ctccccgagc tgatctgcga ccgcctgcgc tgggtggtgc
     7261 tctaccacct ctcccggacc aacaacctcc ccgccctggc cgcggccgcc gtgggcgagg
     7321 ttctcgacag ggagcgttgc ccggcgcaga tggccgtgac cgagcaggac cacccgacac
     7381 cgtggttgga ggtgaatgcg tgaaagcgag agtgaccgtc tatccgcgcc gcgagatcct
     7441 ggacccgcag ggcaaggcga tccgcgacgc gttgtcccgc gtcggcttcg tgggcgtcga
     7501 cgacgtccgc gccggcaaga gcttcgagat ctccctcggc accgacgatc ccggcgaggc
     7561 cgaccgccag ctccgcgaga tgtgcgagaa gctgctcgcc aacaccgtcg tggaggacta
     7621 ctccgtcgag ctcctccgcg gcgagggggc ccgttcatga agtgcggcgt cgtcgtcttc
     7681 cccggcagca actgcgatca cgacgtctac cacgtcctga agcacgtcct cgagcaggac
     7741 accaccttcc tctggcacca ggacgacacg ctcaaggggt gcgacctggt ggtgctcccc
     7801 ggcggcttct cctacggcga ctacctgcgc gccggcgcgc tcgcggccct gtcgccggtg
     7861 atgggcgccg tccgccgcca cgccgaggcg ggcggcctgg tgctcgggat ctgcaacggc
     7921 ttccaggtgc tgctcgagac cgggctcctc cccggcgcga tgcggcgcaa cgccaacctc
     7981 cgcttccagt gccgggacgt gctcctcaag gtggagcgca acgacctccc gttcacccgc
     8041 cactacgagc cggggcaggt gatccgcatc cccatcgccc acgccgaggg gaactacgag
     8101 gacaccgagg agcggctcga cgccctggag gcggcggggc aggtggtctt ccggtacgtc
     8161 accccggacg gcatccccgg cgcccccggc tccgagtgga accccaacgg cgcggcgcgg
     8221 gccatcgccg gcgtctgcaa cccgcagggc aacgtcctcg gcctgatgcc ccacccggag
     8281 cgctgctccg agccgatcct cggcggcacc gacggcctcg ccc
//
LOCUS       DASPJI010000719         5946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_445101, whole genome shotgun sequence.
ACCESSION   DASPJI010000719 DASPJI010000000
VERSION     DASPJI010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445101"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1914)
                     /locus_tag="VEL74_20170"
     CDS             complement(<1..1914)
                     /locus_tag="VEL74_20170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HYG64909.1"
                     /translation="MYKASALVVLAVVSVLSPVHAQSTSPGDTWQQWLRDVGLLIAPK
                     EREAFLALPGDAERRAFAERFWQVRDPFPETPRNELQERWSSGLAEARSRWGTLDDDR
                     SRVFLLQGEPSSGFTARCEGIAGELELWAYEPRFRVKHRSLVVFERQAQGPARIWRPA
                     GDTLPASAQCSGGERLAPELLWLKMVGAEHYRSSVERALLRPKPRQADWYESLVALVP
                     AVPAGEAPSGTFDAGLQVDYPGQVEERRVVRVMMTVPPGSLRPPLPMTAAAGAGGAQD
                     LVLTGQVLRDREVLESFRYKFRVHPEAVEGQGIPLAFERYLLPGFYHLRIKLENLFSK
                     SSFQGERELAVPGPQGKVARALAPPAAPHSAPDGAPGLPDMAGLYSEAAASLGAREPG
                     LHLFGPADNLLIGNIRFEARIDEQPDLPESERVDRVAFLLDGRRILTRNRPPYAVTLD
                     LGPVPRSRTLRVEGLSRDGRVLAADELALNAGAQRFAVQLLEPRPDRAYRRSLRARAE
                     VEPPAGQSVERVEFYFNEDLVATLYQPPFSQPIALPGKDASAGYVRAVAYLSDGLSSE
                     DVVILNAPESPDAMDVRLVELYTAVMDEAGRPVEGLAGGDFQVAEDGVRQTLRRVERV
                     TDIPVRVVELIDNS"
     gene            2117..3229
                     /gene="corA"
                     /locus_tag="VEL74_20175"
     CDS             2117..3229
                     /gene="corA"
                     /locus_tag="VEL74_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HYG64910.1"
                     /translation="MARLGKRSKIIKRYHKPGTAPGTLRPLEEPGPGAEPLRVTVMDY
                     GPQGLVEKAIDKVEDLAAFHKTPSVTWINVEGLNDLPFLEGLGKLFGFHPLALEDVLN
                     CGQRPKVEDYGSYHFIVLKSLRLKPDGKDKDKEKDQDGLELEQISFFLAGNFVITLQQ
                     FHGDSFDAVRERIRRGKGQIRRMGPDYLAYALIDALVDEFFPVLESYGERIEALEDAL
                     VTDPTTETLHEIHQVKRDLLLLRRTAWPERDLLNSLLHEESDIIRPEVQVFLRDCYDH
                     TIQVIDMIETYRDLAAGLLDVYLSSASNRLNEVMKVLTIISTIFIPLSFVASLYGMNF
                     THMPELDEPWGYPAVLAVMFVTGVSMVVYFKRKKWF"
     gene            complement(3233..4234)
                     /locus_tag="VEL74_20180"
     CDS             complement(3233..4234)
                     /locus_tag="VEL74_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64911.1"
                     /translation="MTWRAPRVLAALLVLVLLAGCAPDGASRPGRAAAPAGGTPAAGQ
                     ARKADDPWALVWRRAHGPGGEVVKIGAGMIAIGPVDGQARRYLFAEPERGRELLVFTR
                     TYAPFRLRAGGGVLELRGQGAVKPTPGDRRMIFEWARQVAAEAGGVEPGSGYGLALTW
                     QRVAGSGCDEVAVYLSGEVRTGRCGGNNPISGRLAPGPLAWLYARFDRLAAFQAASQA
                     GPVGLSPRLVFAGRGGETPSSDDRRDLEGFGAALARELAPRPSAPPPPAAPAARGRAA
                     RTPPPPASPAGPRLLLPPQRALPLPEVILPDDRLPPPPPVPPREERQEDGPAPAPPR"
     gene            complement(4231..5541)
                     /gene="mgtE"
                     /locus_tag="VEL74_20185"
     CDS             complement(4231..5541)
                     /gene="mgtE"
                     /locus_tag="VEL74_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HYG64912.1"
                     /translation="MATTLITPDELREAWGLLSPAERLESFGFLVREEAEDFFLELRS
                     ADQLALLLGLPAAARRSWMRLLAPDDAADLLQEATPEVRAELLSLLDAPARQEVSALL
                     AYEEDDAGGLMSPRYARVRPEMTVDEAIGYLRKQAQTVETLHYAYVLDPRQCLLGVIS
                     LRDLFAAPGNARVSEVMQTGLVSVEEQMDQESVSHLLARHDFVALPVVDAEGRMKGIV
                     TFDDIADVIEEEATEDMQKMGGSAALDAPYLQVGLGEMIRKRAGWLSILFVGEMLTAS
                     AMAFYEDAIARAVVLAVFVPLIISSGGNSGSQASTLIVRALALAEVRVRDWWRVVRRE
                     VVAGLGLGSILGVLGLLRVVVWEQAFGTYGEHALQIGLTVGLSLLGVVMWGTLAGSSL
                     PFLLRRLGLDPASASAPFVATLVDVTGLIIYFSFANLLLGGRLL"
     gene            5680..>5946
                     /locus_tag="VEL74_20190"
     CDS             5680..>5946
                     /locus_tag="VEL74_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HYG64913.1"
                     /translation="MDGMDGMTTLRLRINGEDRTVVFPTHHSLLEVLREECGLTGTKH
                     GCELGECGTCAVLVDGRPILSCLSLAAEMGGRAIETVEGLQNGNA"
BASE COUNT          907 a         2187 c         2106 g          746 t
ORIGIN      
        1 ggagttgtcg atcagctcca ccacccgcac ggggatgtcg gtcacccgtt ccacccgccg
       61 gagggtctgc cgcacgccgt cctcggccac ctggaagtcg ccgcccgcca agccctcgac
      121 cggccggccg gcctcgtcca tcaccgcggt gtacagctcc accaggcgga cgtccatggc
      181 gtccggcgac tcgggggcgt tgaggatcac cacgtcctcg gaggagaggc cgtcgctcag
      241 gtaggccacc gcccgcacgt agcccgccga ggcgtccttg cccggcagcg cgatgggctg
      301 ggagaagggc ggctggtaga gcgtcgccac caggtcttcg ttgaagtaga actccacccg
      361 ctcgacgctc tgccccgccg gcggctcgac ctcggcccgc gcccgcaggc tccgccggta
      421 ggcccggtcc ggccgcggct ccagcagttg gacggcgaac cgctgggcgc cggcgttcag
      481 ggccagctcg tcggccgcga ggacccggcc gtcgcggctc agcccctcca cccgcagcgt
      541 ccgcgagcgc ggcaccggcc cgaggtcgag ggtcaccgcg tacggcgggc ggttgcgggt
      601 gaggatccgc cggccgtcga gcaggaaggc gacgcggtcg acccgctcgc tctcggggag
      661 gtccggctgc tcgtcgatcc gggcctcgaa gcggatgttg ccgatcagca ggttgtcggc
      721 gggcccgaag aggtggagcc cgggctcgcg cgctccgagg gaggcggcgg cctcggagta
      781 gaggccggcc atgtcgggga ggccgggggc accgtccgga gcggagtggg gcgccgccgg
      841 aggcgccagg gcccgagcca ccttcccctg cggccccggg acggcgagct cccgctcgcc
      901 ctggaaggag ctcttggaaa agaggttctc cagcttgatc cgcaggtgat agaagccggg
      961 caggaggtag cgctcgaagg ccagggggat gccctgcccc tccacggctt cggggtggac
     1021 ccggaacttg taccggaagc tctccagcac ctcgcggtcg cgcagcacct gcccggtgag
     1081 cacgaggtcc tgggcgccgc ccgcccccgc cgccgccgtc atcggcaggg gaggacggag
     1141 cgagcccggc gggacggtca tcatgacgcg caccacccgc cgctcctcca cctgcccggg
     1201 gtagtccacc tgcagcccgg cgtcgaaggt cccggacgga gcctcaccgg ccggcaccgc
     1261 cggtacgaga gccacgaggc tctcgtacca gtccgcctgc cggggcttgg gccgcaggag
     1321 cgcccgctcg acggagctcc ggtagtgctc cgcccccacc atcttcagcc acagcagctc
     1381 cggcgccagc ctctcgccgc cgctgcactg ggcgctggcc ggcagcgtgt cgccggcagg
     1441 ccgccagatc cgggccggcc cctgcgcctg ccgctcgaaa acgaccagcg accggtgctt
     1501 gacgcggaac cgcggctcat aggcccagag ctccagctcg cccgcgatcc cctcgcagcg
     1561 ggcggtgaac ccggaggacg gctccccctg gagcaggaag acgcgggagc ggtcgtcgtc
     1621 cagcgtcccc cagcggctcc gcgcctcggc gaggccgctc gaccagcgct cctggagctc
     1681 gttgcgcggc gtctccggga aggggtcgcg gacctgccag aagcgctcgg cgaaggcgcg
     1741 gcgctcggcg tcgccgggga gggcgaggaa cgcctcgcgc tccttcggcg cgatcaggag
     1801 gccgacgtcc cgcagccact gctgccaggt gtcgccgggg ctcgtggatt gggcgtggac
     1861 gggggacagg acggaaacga cggccaatac aaccaaggca gacgctttgt acatctttct
     1921 cactctctcc cgggaggagt cggcgagcca tcgcggagcc acggctccgc ttccggcttt
     1981 ctccggggat gcagcaagaa atatgcccct ggggcccctg ggggacgccg cgccggccgt
     2041 cccgcccgac cacaccaccc cttctccgga ctgagacata attccggacg gactccagcc
     2101 acgggggagg agcgagatgg cccgtctggg caagcgcagc aagatcatca aacgttacca
     2161 caaaccgggc accgcgccgg gtaccctccg tccgctcgaa gagccgggtc ccggcgccga
     2221 gccgttgcgg gtgacggtca tggactacgg gccgcagggc ctggtcgaga aggccatcga
     2281 caaggtggag gacctcgccg ccttccacaa gacgccgtcg gtgacctgga tcaacgtcga
     2341 agggctgaac gacctcccct tcctggaggg gctgggcaag ctcttcggct tccaccccct
     2401 ggccctggag gacgtgctca actgcggcca gcgccccaag gtggaggact acggctccta
     2461 ccacttcatc gtcttgaagt cgctgcggct caagcccgac ggcaaagaca aggacaagga
     2521 gaaggatcag gacgggttgg agctggagca gatcagcttc ttcctggcgg gcaacttcgt
     2581 catcaccctc cagcagttcc acggcgactc gttcgacgcg gtccgcgaac gcatccgccg
     2641 gggtaagggg cagatccgca ggatggggcc ggactacctc gcctacgcgc tgatcgacgc
     2701 cctggtcgac gagttcttcc cggtgctcga gagctacggc gagcgcatcg aggcgctgga
     2761 ggacgccctg gtcaccgacc ccaccaccga gaccctccac gagatccatc aggtcaagcg
     2821 cgacctgctc ctgctgcgcc gcaccgcctg gccggagcgc gacctcctca acagcctgct
     2881 gcacgaggag tcggacatca tccggcccga ggtccaggtc ttcctgcgcg actgctacga
     2941 ccacacgatc caggtgatcg acatgatcga gacctaccgc gacctggcgg cgggcctcct
     3001 cgacgtctac ctgtcgagcg ccagcaaccg cctcaacgag gtgatgaagg tgctgaccat
     3061 catctcgacc atcttcatcc cgctgagctt cgtcgccagc ctgtacggca tgaacttcac
     3121 ccacatgccg gagctggacg agccctgggg ctaccccgcc gtcctcgccg tgatgttcgt
     3181 cacgggcgtg tcgatggtgg tctacttcaa acgcaagaag tggttctgag gcctagcggg
     3241 gcggcgccgg ggccgggccg tcctcctgcc tctcctcccg tggcggcacc ggcggcggcg
     3301 gcgggagacg gtcgtcgggc aggatgacct cgggcagcgg cagggcgcgc tgcggcggca
     3361 ggagcaggcg ggggccggcc gggctcgccg ggggaggcgg ggtccgcgcg gccctccccc
     3421 tggcggccgg cgcggcggga ggtggcggcg cgctcggccg gggcgccagc tcccgggcca
     3481 gcgccgcgcc gaagccctcc agatcccggc ggtcgtcgct ggaaggcgtc tcccctcccc
     3541 gcccggcgaa gaccagccgc gggctcagcc ccaccggccc ggcctgggag gcggcctgga
     3601 acgccgccag acggtcgaaa cgggcgtaga gccaggccag ggggcccggc gccaggcggc
     3661 cggagatcgg gttgttcccg ccgcagcggc cggtacggac ctcaccggag aggtagaccg
     3721 ccacctcgtc acagccgctg ccggcgaccc gctgccaggt gagggccagg ccgtagccgg
     3781 agcccggctc caccccgccg gcctcggccg ccacctggcg cgcccactcg aagatcatcc
     3841 gccggtcccc cggagtcggc ttgaccgccc cctggccgcg gagctccagc acgccgccgc
     3901 cggcgcggag ccggaacggc gcgtaggtgc gggtgaagac gaggagctcg cggccgcgct
     3961 ccggctcggc gaagaggtag cggcgcgcct gcccgtccac cggcccgatg gcgatcatgc
     4021 cggcgccgat cttcaccacc tccccgccgg ggccgtgggc ccgtcgccag acgagagccc
     4081 aagggtcgtc cgccttccgc gcctgcccgg ccgcgggcgt ccccccggcg ggcgccgccg
     4141 cccgcccggg ccgggaggct ccgtccggcg cgcagcccgc gaggagaacc aggaccagga
     4201 gggccgcgag gacgcgcggg gccctccagg tcatagcagc ctccccccca gcagcaggtt
     4261 ggcgaacgag aagtagatga tcaggccggt gacgtccacc agcgtcgcca cgaagggggc
     4321 cgaggcgctg gccggatcga gcccgagccg gcgcagcagg aacggcaggc tggagccggc
     4381 gagcgtgccc cacatcacga ccccgaggag gctcaggccg acggtgaggc cgatctgcag
     4441 ggcgtgctcg ccgtaggtgc cgaacgcctg ctcccagacc accacccgca gcaggccgag
     4501 cacccccagg atgctcccca ggccgaggcc ggcgacgacc tcgcggcgca ccacccgcca
     4561 ccagtcgcgc acccgcacct cggcgagcgc cagggcgcgc acgatcagcg tcgaggcctg
     4621 ggagcccgag ttgccgccgc tgctgatgat cagcggcacg aagaccgcca gcaccacggc
     4681 ccgggcgatg gcgtcctcgt agaaagccat ggccgaggcg gtgagcatct cgccgacgaa
     4741 caggatcgac agccaccccg cccgcttgcg gatcatctcc cccaggccga cctgcagata
     4801 gggggcgtcg agcgccgccg agccgcccat cttctgcatg tcctcggtgg cctcctcctc
     4861 gatgacgtcg gcgatgtcgt cgaaggtgac gatacccttc atccgcccct cggcgtcgac
     4921 caccggcagc gccacgaagt cgtgccgggc cagcagatgg ctgacgctct cctggtccat
     4981 ctgctcctcg accgacacca ggccggtctg catcacctcg gagacccggg cgttgccggg
     5041 ggcggcgaac aggtcgcgca gcgagatcac ccccagcagg cactgccggg gatcgagcac
     5101 gtaggcgtag tggagggtct ccacggtctg cgcctgcttg cgcaggtaac cgatggcctc
     5161 gtcgaccgtc atctcggggc ggacgcgggc atagcgcggg ctcatcaggc cgcccgcgtc
     5221 gtcctcttca taggcgagga gggcgctcac ctcctggcgg gccggcgcgt cgaggaggga
     5281 gagcagctcg gccctgacct ccggggtcgc ctcctgcagc aggtcggcgg cgtcgtccgg
     5341 cgccagcagc cgcatccacg accgccgcgc cgccgcgggc agtccgagga ggagggcgag
     5401 ctggtcggcc gagcgcagct ccaggaagaa gtcctcggcc tcctcgcgca ccaggaagcc
     5461 gaagctctcc agccgctcgg ccggagagag gagcccccag gcctcgcgca gctcgtcggg
     5521 ggtgatcagg gtcgtcgcca tgggtggagc ctcggtccgc acgccgtcgc actcggcatg
     5581 ggctaacata tcccgcccca ggccgccaga gaaggtgccg gagaaggatc ggcggggaag
     5641 taaaatacgg cggcggaggc gcgaggaaat caccccatca tggacggaat ggacggcatg
     5701 accaccctga ggctgcggat caacggcgag gaccggaccg tcgtcttccc cacccatcac
     5761 tccctgctcg aggtgctgcg ggaggagtgc ggcctgaccg gcaccaagca cggctgcgag
     5821 ctgggcgagt gcggcacctg tgccgtgctg gtggacggcc gccccatcct ctcctgcctg
     5881 tcgctcgccg ccgagatggg cgggcgggcc atcgagaccg tcgaggggct gcagaacggc
     5941 aacgcg
//
LOCUS       DASPJI010000720         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_790290, whole genome shotgun sequence.
ACCESSION   DASPJI010000720 DASPJI010000000
VERSION     DASPJI010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790290"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /gene="radC"
                     /locus_tag="VEL74_20195"
     CDS             <1..532
                     /gene="radC"
                     /locus_tag="VEL74_20195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HYG64914.1"
                     /translation="ELLRENGGLSGLVGTGPLSLRRRGLGPAKAASLLAAVEIGRRLA
                     RDQLLDREPLSRPAEVARYLSLRYRQRDQEVLGALFLDKKKCLLGEREIYRGTLDRAA
                     VEPREILKECLLRGAAGVLIFHTHPSGDPSPSNDDILFTQHLARAASVIGITLVDHMV
                     LASSGRWVSLKERVAW"
     gene            545..1885
                     /gene="larC"
                     /locus_tag="VEL74_20200"
     CDS             545..1885
                     /gene="larC"
                     /locus_tag="VEL74_20200"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HYG64915.1"
                     /translation="MAHYHLDCFSGLAGDMFLGACLDLGMPLEVLTDTVARLGLPGVS
                     VEARKAMRGGFTGTRFRVLRDSRPIEGPDPEEQQHHHHEHHHHHHHHPHDHGHGHHQH
                     HDHGHDHHHHDHDHGHHHHHEHGRDLPAIRRLIQESGLAGPVQERALRLFQRLGEAEA
                     KAHGMPIEQVHFHEVGAVDSIIDLVGAAAAIEHLAPERITCGPVNVGSGTVRTAHGEL
                     PVPAPATAELLRGVPIFGGPGGELTTPTGAVLLAELVNEFVEAPAFILDRVGYGLGKK
                     HLPHQPNATRLLAGKGTQVRPEVMVVECEVDDLPGEAFGFLMERLLEAGALDVYFTPV
                     QMKKNRPGTLVTLLCRRQQLEPLAGVLLMESGSLGCRYYLASRFEAERDVVDVETPYG
                     AVRVKRARLDGHPLATAPEFEDCRRLALAAGVPLRDVYRAALTAAGSVGSGQWR"
     gene            1907..2794
                     /gene="hslO"
                     /locus_tag="VEL74_20205"
     CDS             1907..2794
                     /gene="hslO"
                     /locus_tag="VEL74_20205"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HYG64916.1"
                     /translation="MSVNETETAAQGRLELGLAGEGALRWAVVNVTDVVEEARERLDL
                     SPVAAAALGRSLAAAALLLRMSTKTPTRLVLEVRGDGPLGRVLAEVDQDGNLRGMVGN
                     AHVDLPPTPAGKLDVGAAVGSGFLRVLREHEGGSYHSQVELVSGEIGDDVAHYLEQSE
                     QSRSAVLLGVLAKPSGIAAAGGMIIEVLPGAPDEVVQQLESNIAGTIGVSHLVEAGGI
                     PYVVDTLLAGMDRTVKETRPLRFRCRCSRERLLHHLILLSTDDKDHLSEEDGSIEADC
                     VFCGAHYRFATEELNPRPN"
     gene            2813..>3109
                     /locus_tag="VEL74_20210"
     CDS             2813..>3109
                     /locus_tag="VEL74_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009850811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HYG64917.1"
                     /translation="MASFYVTTPIYYVNSLPHIGHIFTTTVADVLARYRRMAGEEVYF
                     LTGTDEHGQNIERAAQKEGIAPIELADRVVAQYRELRGRLGFSNDDFIRTTEERH"
BASE COUNT          474 a         1064 c         1097 g          474 t
ORIGIN      
        1 ggagctgctg cgggagaacg gcgggctctc cgggctggtg gggacggggc cgctctcgct
       61 acggcggcgc ggcctcgggc cggccaaggc cgcgagcctg ctggcggcgg tcgagatcgg
      121 ccgccggctc gcccgtgacc agctcctgga ccgcgagcct ctgtcgcgtc cggctgaggt
      181 ggcgcgctac ctctccctgc gctatcgcca gcgcgaccag gaggtactgg gggcactgtt
      241 cctggacaag aagaaatgcc tgctgggaga gagggagatc taccgcggca ccctcgaccg
      301 ggcggcggtg gagcccaggg agatcctcaa ggagtgcctg ctgcgcggcg cggccggggt
      361 tctcatcttc catacccacc cgagcggcga tccgtcaccc agcaacgacg acatcctgtt
      421 cacccagcat ctcgcccgag ccgcatcggt gatcgggatc acgctggtcg atcacatggt
      481 gctcgccagc tcgggccgct gggtttccct caaggaacgg gtcgcgtggt agcctaaccg
      541 gcccatggcg cactatcacc tcgactgctt cagcggactc gccggcgaca tgttcctggg
      601 tgcctgtctg gacctgggga tgccgctgga ggtgctcacc gacacggtgg cccgcctggg
      661 cctgccgggc gtctcggtcg aggcgcgcaa agccatgcgc ggcggcttca ccggtacccg
      721 cttccgggtc ctccgggata gccggcctat cgaggggccc gatccggagg agcagcagca
      781 tcatcatcac gagcaccacc accaccacca ccatcatcct catgatcatg gccacggcca
      841 tcatcagcac catgatcacg gccatgacca ccatcaccat gatcatgacc acgggcacca
      901 tcaccaccac gagcacggcc gggacctgcc ggcgatccgc cggctgattc aggagagcgg
      961 cctggcggga ccggtccagg agcgggcgct gcggctgttc cagcggctgg gggaggcgga
     1021 ggcgaaggcg catgggatgc cgatcgagca ggtccatttt cacgaggtcg gggcggtgga
     1081 ctccatcatc gacctggtgg gggctgcggc ggccatcgag cacttggcgc cggagcgcat
     1141 cacctgtggg ccggtcaacg tcgggagcgg cacggtgcgg acggcccacg gcgagctccc
     1201 ggtgccggcg ccggccacgg ccgagctgct gcggggggtg cccatcttcg gcggccctgg
     1261 cggcgagctg accactccca cgggcgccgt gctcctggcc gagctggtga acgagttcgt
     1321 ggaggccccg gctttcatcc tcgatcgggt ggggtatggg ctggggaaga agcatctgcc
     1381 gcaccagccc aacgccacgc gcctgctggc cggcaagggg acgcaggtac ggccggaggt
     1441 gatggtcgtc gagtgcgagg tggacgacct gccgggggag gcgttcggct tcctgatgga
     1501 gcgcctgctc gaagccgggg ccctggacgt ctatttcacc ccggtgcaga tgaagaagaa
     1561 ccgcccgggg accctggtca ccctactctg ccgccgccag cagctcgagc cgttggcggg
     1621 cgtccttctc atggaatctg gctcgcttgg atgccgttat tacttagcgt cgcgtttcga
     1681 agcggagcgc gatgtggtgg atgtggagac gccgtacggg gcggtgcggg tcaagcgcgc
     1741 ccgtctcgat ggccatcccc tcgccaccgc gcccgagttc gaagactgcc ggcggctggc
     1801 actcgccgcc ggcgtgcctc tgcgcgatgt ctatcgcgcc gcccttaccg ctgcgggctc
     1861 ggtcggctcc ggtcagtggc gttagtcgaa ggaaagcgag tccaggatgt ctgtgaacga
     1921 gacggagacg gcggcccagg gccgcctgga gctgggcctt gccggagagg gggctctccg
     1981 ctgggccgtg gtgaacgtga ccgacgtggt cgaggaggcg cgggagcggc tggacctctc
     2041 gccggtggcc gcggcggcgc tgggacggtc cctcgctgcg gcggcgctgc tgctgcgcat
     2101 gtccaccaag actccgaccc ggctggtgtt ggaggtgcgg ggggacggcc ccctcggccg
     2161 cgtcctcgcc gaggtcgacc aggacgggaa cctgcgcggc atggtgggca acgcccacgt
     2221 ggacctgccg cccacgccgg ccggcaagct ggacgtcggg gcggccgtcg ggagtggttt
     2281 cctccgcgtg ctccgcgagc acgagggggg gagctatcac agccaggtgg agctggtgag
     2341 cggcgagatc ggcgacgacg tcgcccacta cctggagcag agcgagcaga gccgctcggc
     2401 cgtgctcctc ggcgtgctcg ccaagccgag cggcatcgcc gccgccggcg gaatgatcat
     2461 cgaggtcctg cccggcgcgc ccgacgaggt cgtgcagcag ctcgagagca acatcgccgg
     2521 caccatcggc gtgagccacc tggtggaggc cggcggcatc ccctacgtcg tcgacacgct
     2581 gctcgccggc atggaccgca cggtcaagga gacgcggcct ctccggttcc gctgccgttg
     2641 cagccgcgag cgcctgctgc accaccttat cctgctgtcg accgacgaca aggatcacct
     2701 gagcgaggag gacggctcca tcgaagcgga ctgtgtcttc tgtggcgccc actaccggtt
     2761 cgccaccgag gagctgaacc ctcgtcccaa ctgacataga atcccccctc ccatggcctc
     2821 cttctacgtc accaccccga tctactacgt gaacagcctg ccccacatcg ggcacatctt
     2881 cacgaccacc gtggccgacg ttctggcccg ctaccggcgc atggccggcg aggaggtcta
     2941 cttcctcacc ggcaccgacg agcatggcca gaacatcgag cgtgcggccc agaaggaggg
     3001 gatcgcgccg atcgagctcg cagaccgtgt cgtggcccag taccgcgagt tgcgcggccg
     3061 gctcggcttc tcgaatgacg acttcatccg caccaccgag gagcgccac
//
LOCUS       DASPJI010000721         2366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_392870, whole genome shotgun sequence.
ACCESSION   DASPJI010000721 DASPJI010000000
VERSION     DASPJI010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392870"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1775
                     /locus_tag="VEL74_20215"
     CDS             <1..1775
                     /locus_tag="VEL74_20215"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HYG64918.1"
                     /translation="AADLHVFAPGGGELPVRVEPWLPRAERREAVVTDVQKTDTGWTL
                     LLDTGTERLSHDGLLFDFDRATTVPAVRLEGSADGEVWRPLAVGDLFRVGEERGLQRI
                     SLSYPASPDRYLRLAWPEAGGYPRVSRVEVETAPGPSVTLTTRDVACEPRQGAAVSGV
                     VCQLALPAPGQILRHITLEIEGSGTVGYLLDEPRDARWQPLARGVWQRAAGGGAASRH
                     RLLDDPREVAGSTLRLQLHGPGEPPELVSFGVEMAVQTVVFEAGEPGLYTLAYGGTAR
                     RATGYEGDGAAAWVEAGPETAHPVPPLPPPLAQIAAPGARLPRARFAASWDVVAPSAR
                     PGDLVRLEIPDAVYGTARDDLGDLRLEMAGRQIPFFRWSPPEPAPAGAREDARPAESG
                     EEGESQIGLSLPVSGLPLTELDLWIGARPLRRPVTVRSVDNRRRSSRQEETLARETWE
                     CTPEPPLPCRALLPLSPLPRTASGLLTVRFEDGDDPPLSGLDLELWRRRDVLLFAWPP
                     DQTAKGPVRLLAGAESLGAPDYDLAALGDVLLSRAWQPAELDLAGTLGPGEAPWWSRW
                     VMPVALIVAGTFLLLLLRRILSNS"
     gene            complement(1835..>2366)
                     /locus_tag="VEL74_20220"
     CDS             complement(1835..>2366)
                     /locus_tag="VEL74_20220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64919.1"
                     /translation="RGIKRSLRPAAAPSEVRDLTGTMTPAASPDPLAPLFQKGLHPEQ
                     WCWEQCSADYDACPCDYGDCSRCDAQWQGCLSSCPTVCYEPKSITSTNKTDIIGITYG
                     YSECLESLWENDFQSGEWYQSTTYQFKHYTLRRTESCNGTVTYQETNVSYSSRTCQSR
                     VGGSCSYPIFRASNAC"
BASE COUNT          293 a          857 c          878 g          338 t
ORIGIN      
        1 gggcggcgga cctgcatgtc ttcgcgccgg gtggtggcga gctgccggtg cgggtcgagc
       61 cctggctgcc gcgcgccgag cggcgcgaag ccgtggtcac cgacgtccag aagaccgata
      121 ccggctggac gctgctcctc gacaccggca ccgagcgcct ctctcacgac ggcctgctct
      181 tcgacttcga ccgggccacc accgttcccg ccgtgcgtct cgaagggagc gcggacggcg
      241 aggtctggcg cccgctggca gtgggcgacc tgttccgggt gggggaggag cgcgggctgc
      301 agcggatctc cctctcctat ccggcgagcc cggaccgcta cctgcggctc gcctggccgg
      361 aggccggcgg ctacccgcgg gtctcgcggg tcgaggtgga gacggcgccc ggcccctcgg
      421 tcaccctgac cacccgcgac gtcgcctgcg agccgcgcca gggcgccgct gtctccggag
      481 tggtctgcca actggccttg ccggcccccg gccagatcct ccgccacatc actctggaga
      541 tcgagggctc cgggaccgtc ggctatctgc tggacgagcc gcgcgacgcc cgctggcagc
      601 cgctcgcccg gggcgtctgg cagcgggctg ccggcggcgg tgccgccagc cggcaccgcc
      661 tgctggacga tccgcgggag gtcgccggct ccaccctccg gctgcagctc cacggccctg
      721 gtgaaccacc ggagctggtg agcttcggag tcgagatggc ggtgcagacc gtggtcttcg
      781 aggccggcga gccggggctc tacaccctgg cctacggcgg caccgcccgc cgcgcgacgg
      841 ggtacgaagg ggacggagcg gcggcgtggg tcgaagccgg accggagacg gcgcatccgg
      901 tgccgccgct gccgccgccg ctggcccaga tcgcggcgcc gggcgcgcgc ctccccaggg
      961 cgcgcttcgc cgcgtcctgg gacgtggtgg cgccgtcggc ccggcccgga gacctggtgc
     1021 ggctggagat cccggacgcg gtctacggca ccgcccggga cgacctgggg gacctgcggc
     1081 tcgagatggc cggccggcag atccccttct tccgttggtc gccgcccgag ccggcgccgg
     1141 ccggcgcgcg ggaggacgcg cggccggccg agagcggcga ggagggggag agccagatcg
     1201 ggctctcgct gccggtctcg ggcctgccgc tgaccgagct cgacctgtgg atcggcgccc
     1261 gccccctccg ccgtccggtg accgtgcgct ccgtcgacaa ccggcggcgt tcctctcggc
     1321 aggaggagac cctggcccgg gagacctggg agtgcacccc cgagccgccg ctcccctgcc
     1381 gggcgctgct ccccctgtcg ccgctgccga ggaccgcgtc ggggctgctc accgtgcgct
     1441 tcgaggacgg cgacgacccg ccgctctccg gcctcgacct cgagctgtgg cggcggcgcg
     1501 acgtgctgct cttcgcctgg ccccccgatc agacagccaa ggggccggtc cgcctcctgg
     1561 ccggggccga gagcctcggc gcgccggact acgacctcgc cgctctggga gacgtcttgc
     1621 tctcccgcgc ctggcagccg gccgagctcg acctcgccgg caccctcgga ccgggcgagg
     1681 ccccctggtg gagccgctgg gtcatgccgg tggcgctgat cgtggcgggg acctttctct
     1741 tactgctgct gcggcggatc ctctccaact cctgaagcag cgaaggggcc acccgcgcgg
     1801 cgggcagccc ctccacctcg atcccgggac gggatcagca ggcgttcgag gctctgaaga
     1861 tcggatagga gcagctcccc cccacgcggg actggcaggt ccgcgaggag tacgagacgt
     1921 tggtctcctg ataggtcacg gtgccgttgc aggattcggt gcggcggagg gtgtagtgct
     1981 tgaactggta cgtggtcgac tggtaccact cgcccgactg gaagtcgttc tcccacaagc
     2041 tctccaggca ctcggaatac ccgtacgtga tgccgatgat gtcggtcttg ttggtgctgg
     2101 tgatgctctt gggctcgtag cagacggtgg ggcaggacga gaggcaaccc tgccactgcg
     2161 catcgcagcg gctgcagtcg ccgtagtcac aagggcaggc gtcatagtcg gccgagcact
     2221 gctcccagca ccattgctcc ggatggagcc ccttctggaa aagaggcgcc aggggatccg
     2281 gagaggcggc gggggtcatg gtaccggtca ggtcccggac ctccgaaggc gccgcggccg
     2341 gacggagcga gcgcttgatg ccccgg
//
LOCUS       DASPJI010000722         8642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_270354, whole genome shotgun sequence.
ACCESSION   DASPJI010000722 DASPJI010000000
VERSION     DASPJI010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8642)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270354"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VEL74_20225"
     CDS             complement(<1..596)
                     /locus_tag="VEL74_20225"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HYG64920.1"
                     /translation="MRQYLDLLQHILDQGIEKSDRTGTGTLSVFGYQMRFDLAAGFPL
                     LTTKKLHTRSIIHELLWFLSGSTNVRPLQENGVTIWDEWADENGELGPVYGRQWRAWP
                     TPDGGAIDQISQVVEQIRRNPDSRRHVVSAWNVAQIDEMKLPPCHALFQFWVGRGRLS
                     CQLYQRSADVFLGVPFNIASYALLTLMVAQATDLEPGE"
     gene            676..1290
                     /locus_tag="VEL74_20230"
     CDS             676..1290
                     /locus_tag="VEL74_20230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HYG64921.1"
                     /translation="MSKSIGCLLAIGAVVLLAVLYGVGVYNGLVGAEQAVEAQWAQVE
                     AAYQRRADLIPNLVATVRGSAAFERETLEAVVQARSRVGQISAQVGSEILNDPQRFEQ
                     FQQAQEGLNSALGRLIAVAESYPQLQSTTAFRDLMVQLEGTENRITVERNRFNEVAQG
                     YNTRVRRFPAALFAGMFGFEPKPYFRSQPGAETAPRVDFGTAPR"
     gene            1350..2597
                     /locus_tag="VEL74_20235"
     CDS             1350..2597
                     /locus_tag="VEL74_20235"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYG64922.1"
                     /translation="MIGRSLSLLFLALLWLGIAGGCAGPSGAEGERSGSGAAGGAEMA
                     VRRGDLQERMLVTGELVAEQAAPLDVPRTRAFQLQIRWIAEDGTPIKAGQPVVEFDNS
                     SFASDLEEKKLSVSEAVNELARMQAEGESNLSEKAFVVQEKRTALEKARTEAVVPRDL
                     LTLREYQERQSALERAEVELAKAEKDLAAFRQTQAADLEVKRIDLERSRREIQEAERA
                     IQVLAVRSPRDGLVLASEHPWEGRKFQEGDTVWPGMTVASIPDLSSLRVEAALPDVDD
                     GRVRPGMRVVCYLDAYPSLAFPGRVVEIAPVARESAYNSLRRYFAMKVDLDRVDTGRM
                     RPGMSVRVEVLGRGVKDALLVPRAALDLSGKSPRALLASGGATPVRLRLCTATTCAVE
                     GLQVGTRLRSATSSNDAGAGRTG"
     gene            2594..3826
                     /locus_tag="VEL74_20240"
     CDS             2594..3826
                     /locus_tag="VEL74_20240"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYG64923.1"
                     /translation="MTPRGKRIGLLVALAVLAAVAVAGWVFSGRSAAADGEDWVEVQR
                     EDLVTGVEVSGTLGAVQSVQLGPPQVPGLYDYKLAFMAPEGMEVRQGQPVLGFDSSEL
                     ERTLLEKMAERDQAQTELEKRQTDLDLQLGEARMQLAEAEATQRRAALKADVPADLVA
                     GNELQQNKADLELAEKRIAFNKERLVLRERQGRAELDALRNKRDRAAQRVAETEAAIQ
                     KMTIVSPRDGTVVYISNRGEKKKVGDTCWNSEKVIEIPDLRRMKAEGQVDEADAGRVE
                     VGQPVRLALDAHPDVTFTGRVRAIRGAVGRKSRFNALKVVALELELDRTDPQRMRPGM
                     RFLGTIEIDRAPEALVIPAEAVANRAGGPVVYRRGRWEAEEVPVKLGRRNERLVEVLD
                     GLSAGDRVLRRPPAGEDA"
     gene            3823..5154
                     /locus_tag="VEL74_20245"
     CDS             3823..5154
                     /locus_tag="VEL74_20245"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYG64924.1"
                     /translation="MKLSLPRQRRGLLLVLLLALVLVVGVGPFLPLAGSLGSGRFGDV
                     PLYRVQRQAFERRVPAQGELRAVRATPINVPSDAPGPFRIAWLAPDGSRVKAGEVVVR
                     FDSSEIEKTLINAEADLASARWRSQKQRVHGSAQVEKLERDLQLARMELDNARQFQKK
                     DAQIFSRNDIIESEIDSDLAQHRERHAREARRTEESLSSSELQLIAIDMRQAELKIQQ
                     ARQALTALAVTAPHDGVLVMNRDWRGTPTRVGDSVWNGQPLAQLPDLSTMEAEVFVLE
                     ADAGGLEAGKPATVVVESAPGVSYQAKIRRVEALAKPRLRGSPVQYFAVTLALDRTDP
                     RVMKPGQRVRANLLLEERQGALTVPRQAVFQSEGRTVVHRKKGDGFEAVEVKLGSSGA
                     GRVVVESGIGEGDLLALVDPARPVGEDATAEEEASPTPGRPSAPVAPGGAR"
     gene            5151..6383
                     /locus_tag="VEL74_20250"
     CDS             5151..6383
                     /locus_tag="VEL74_20250"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG64925.1"
                     /translation="MNPREAFRSALENLAGHKLRSALTMLGMIFGVGAVIAMLSIGAG
                     AEEQALGMIERLGLRNVLIRAREYKTEELQEIRQKSLGVSFRDAAAISEAVPGVDLVA
                     PRIRIEPYKVLSAAGKTEATVFGVSHHHAGLADLELAEGRFLDALDESTHAQVCVIGP
                     GVRRDLFGYGPAVGEEVKVDDVWLEVIGVLAATGQGETFEGVPVGSVETEIYLPVTTA
                     ERKFERPPLDSPLDEIVVRLKEGQSGREAAAVISGLLSRLHAGTDDYEIVVPEALLEQ
                     SRRTQRLFNVVMGCIAGISLLVGGIGIMNIMLATVLERTREIGVRRAVGARRSDIRFQ
                     FVVESFAISLIGGLAGVIAGIAIARGVAVYAGWETIVTVGSILISTGVAMTVGLASGI
                     YPATRAAGLDPIEALRYE"
     gene            6761..8119
                     /gene="dnaB"
                     /locus_tag="VEL74_20255"
     CDS             6761..8119
                     /gene="dnaB"
                     /locus_tag="VEL74_20255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HYG64926.1"
                     /translation="MIETLTQQKSLPSSEESERAVLGGVMLDPAVLPTISGRLRPEDF
                     YLERHQILYQAMIDLQEELVDIDLRTLQAKLEQQGKFGTVGGLSYLTGLDLDLPDIGR
                     IDAYVEIVKERSVRRRLIQASSQIIRDCLDGGLSAQAALGRAEQAILGLGEEAIQKGF
                     ALLGDVFHTTLEEIEERPGSMLTGIPTGYIDLDRITQGLNKGNLIIIAGRPGMGKTSF
                     ALNVAQHVAIRERRTVGIFSLEMSQQELALRILCSESDISFARLRAGRISQKEWTKIM
                     TTVRGVGDAPLYIDDSPNPTLLEVASKSRRLKAEKGLGVLVLDYLQLMQAGGKYENRN
                     LEIAAISRGLKQLAKELEIPVIALSQLSRNPDRRGADHRPQLSDLRESGSIEQDADMV
                     AFIYRDEVYNPTEENKGLAELIIAKHRNGETGTVEMVFLGETTSFRNLDRHGAPDVPA
                     PF"
     gene            8100..>8642
                     /locus_tag="VEL74_20260"
     CDS             8100..>8642
                     /locus_tag="VEL74_20260"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HYG64927.1"
                     /translation="MSQHRSDALLTKGEELASALRPAWVDVDLDALGHNLQVIRRQTP
                     GTRLMAVVKADAYGHGAAAVSRALAGAGVDWLGVALLEEGAKIRRAGVETPILVLGTA
                     RPAKIALYQRYRLVPTVSSLAELGLWRDWVGTRAEPQPIHLKVDTGMGRLGVAVERAP
                     ELVREATSSGIQVVGLMTHLA"
BASE COUNT         1312 a         3029 c         3078 g         1223 t
ORIGIN      
        1 aactcgcccg gttccaggtc cgtggcctgc gcgaccatca gcgtgagcag cgcgtatgac
       61 gcgatgttga acggcacgcc gaggaagacg tcggcgctgc gctggtagag ctggcacgag
      121 agccggcccc ggccgaccca gaactggaac agggcatggc agggggggag cttcatctcg
      181 tcgatctggg cgacgttcca ggccgagacc acgtggcggc gcgagtccgg attgcggcgg
      241 atctgctcga ccacctgcga gatctgatcg atggcaccgc cgtcgggagt gggccaggcg
      301 cgccactgcc ggccgtagac cggtcccagc tcgccgttct cgtccgccca ctcgtcccag
      361 atggtgacgc cgttctcctg gagaggccgg acattggtgc tgccggacag gaaccagagc
      421 agctcgtgga tgatggagcg ggtgtgcagc ttcttcgtgg tcagcagggg gaaacccgcc
      481 gccaggtcga aacgcatctg gtacccgaag acactcaagg tgccggtgcc ggtccggtcg
      541 ctcttctcga taccttgatc caggatgtgc tggagcaggt cgaggtactg gcgcatgcgc
      601 ggcactctaa catggccggg atatcatctt gggcggccgg cgtcccgccg tgcccgtctg
      661 gaaagagggg ggtctatgag caagtcgatc gggtgtctgc tcgccattgg tgccgtggtg
      721 ctgctcgcgg ttctctacgg cgtcggggtg tacaacggcc tggtgggggc cgagcaggcg
      781 gtggaggccc agtgggccca ggtggaggcc gcctaccagc gccgcgccga cctgatcccg
      841 aacctggtgg ccacggtccg cgggtccgcc gccttcgaac gggagacgct cgaggccgtc
      901 gtccaggccc gcagccgcgt cgggcagatc agcgcccagg tgggcagcga gatcttgaac
      961 gacccgcaac gcttcgagca gttccagcag gcccaggagg ggctcaactc cgcccttggg
     1021 cggctgatcg cggtggccga gagctatccc cagctgcagt ccacgaccgc gttccgggac
     1081 ctcatggtcc agctcgaggg gacggagaac cggatcaccg tcgagcgcaa ccgcttcaac
     1141 gaggtggccc agggctacaa cacccgggtc cggcgcttcc cggcggcgct cttcgccggc
     1201 atgttcggct tcgagcccaa gccctacttc cggtcgcagc cgggggcaga gaccgccccg
     1261 cgggtcgatt tcggcacggc tccgcgctga caggaggccg gcggctcaac gcggaatacg
     1321 tctggcacgt atatgttagt gaagagatga tgatcggacg ctccctctcc ctgctgttcc
     1381 tcgccctgct ctggctcggg atcgccggcg gctgcgccgg ccccagcggc gccgaggggg
     1441 agcggtccgg ctcaggcgcg gccggtggcg ccgagatggc ggtccgccgg ggagacctcc
     1501 aggagcggat gctggtgacc ggcgagctgg tggcggagca ggccgcgccg ctcgacgtcc
     1561 cccggacccg cgccttccag ctccagatcc gctggatcgc cgaggacggc acgccgatca
     1621 aggccgggca gccggtggtg gagttcgaca actcctcgtt cgcctcggac ctggaggaga
     1681 agaagctgtc ggtctccgag gcggtcaacg agctggcgcg catgcaggcg gagggggaga
     1741 gcaacctctc cgagaaggcc ttcgtggtgc aggagaagcg gaccgcgctg gagaaggccc
     1801 gcaccgaggc cgtggtcccc cgcgacctcc tcaccttgcg ggagtaccag gagcggcaga
     1861 gcgccctcga gcgcgccgag gtggagctcg ccaaggcgga gaaggacctc gccgccttcc
     1921 gacagaccca ggccgccgac ctcgaggtca agcgtatcga cctcgagcgg tcgcggcgcg
     1981 agatccagga ggccgagcgc gccatccagg tcctcgccgt gcggtccccc cgcgacggtc
     2041 tggtgctcgc cagcgagcac ccctgggagg gccgcaaatt ccaggaaggg gatacggtct
     2101 ggccgggcat gaccgtggcc tccatccccg acctctcgtc gctgcgcgtc gaggccgccc
     2161 tgcccgacgt ggacgacggc cgggtgcgcc ccggcatgcg cgtcgtctgc tacctcgacg
     2221 cctatccgtc tctcgccttc ccgggccggg tcgtggagat cgcgcccgtg gcccgcgagt
     2281 ccgcctacaa ctcgctgcgg cgctacttcg ccatgaaggt ggacctcgac cgtgtcgaca
     2341 ccggacggat gcgcccgggc atgtcggtgc gggtggaggt tctcggccgt ggcgtcaagg
     2401 acgcgctgct ggtgccgcgc gccgccctgg acctctccgg caagtcgccg cgggccctcc
     2461 tggcgagcgg cggcgcgacg ccggtgcgtc tgcgcctctg caccgccacc acctgcgcgg
     2521 tcgagggcct ccaggtaggg acgcggctcc gctccgccac ctcttcgaat gacgctggcg
     2581 cggggaggac cggatgacgc cgcgggggaa gcggatcggc ctcctcgtcg ccttggccgt
     2641 gctggccgcg gtcgccgtgg ccggctgggt cttcagcggc cggagcgccg ccgccgatgg
     2701 agaggactgg gtggaggtcc agcgcgagga cctggtgacc ggcgtcgagg tctccggcac
     2761 cctgggcgcc gtgcagagcg tgcagctcgg cccgccgcag gtccccggcc tctacgacta
     2821 caagctcgcc ttcatggccc ccgaggggat ggaggtccgt cagggacagc cggtgctcgg
     2881 cttcgactcc tccgagctgg agcgcacgct gctcgagaag atggccgagc gggaccaggc
     2941 ccagaccgag ctcgagaagc gccagaccga cctcgacctg caactcggcg aagcgcggat
     3001 gcagctcgcc gaggccgagg ccacccaacg ccgcgccgct ctcaaggcgg acgtccccgc
     3061 cgatctggtg gccggcaacg agctccagca gaacaaggcc gacctcgagc tggccgagaa
     3121 acgcatcgcc ttcaacaagg agcggctggt cctgcgcgag cgccaggggc gggccgagct
     3181 ggacgccttg cgcaacaagc gcgaccgggc cgcgcagcgg gtggccgaga ccgaagcggc
     3241 catccagaag atgaccatcg tgtcgccccg cgacggcacc gtggtctaca tctccaaccg
     3301 cggcgagaaa aagaaggtgg gggacacctg ctggaactcg gagaaggtga tcgagatccc
     3361 cgacctgcgc cggatgaagg ccgaggggca ggtggacgag gcggacgccg gccgggtcga
     3421 ggtgggccag ccggtgcgtc tggccctgga cgcccatccg gacgtgacct tcaccggccg
     3481 cgtgcgcgcc atccgcggcg ccgtcggcag gaagtcccgt ttcaatgccc tcaaggtggt
     3541 cgccctggag ctggagctcg accgcaccga tccgcagcgc atgcgtccgg gcatgcggtt
     3601 cctcggcacc atcgagatcg accgcgctcc ggaggcgctg gtgatccccg ccgaggcggt
     3661 ggccaatcgc gccggcggtc cggtggtcta ccggcgcggg cgctgggagg cggaggaggt
     3721 cccggtcaag ctgggccgca ggaacgagcg gctggtggag gtcctggacg gcctctcggc
     3781 cggtgaccgg gtgctgcggc gcccgccggc gggggaggac gcgtgaagct gtcccttccc
     3841 cggcagcggc ggggcctcct gctggtgctg ctgctcgccc tggtcctggt ggtgggtgtg
     3901 ggccccttcc tccccctcgc cggctcgctg gggagcggcc gcttcggcga cgtccccctc
     3961 tatcgcgtgc agcgccaggc gttcgagcgg cgggtgcccg cccagggtga gctgcgggcg
     4021 gtgcgcgcca cgccgatcaa cgtgccgagc gacgcgcccg gccccttccg tatcgcctgg
     4081 ctggcgcccg acggcagccg cgtcaaggcc ggcgaggtgg tggtgcgctt cgactccagc
     4141 gagatcgaga agaccctgat caacgccgag gcggacctgg cgagcgcccg ctggcgctcg
     4201 cagaagcagc gcgtccacgg ctcggcccag gtggagaagc tggagcgcga cctgcagctt
     4261 gcccgcatgg agctcgacaa cgcccgccag ttccagaaga aggacgcgca gatcttctcg
     4321 cgcaacgaca tcatcgagtc ggagatcgac agcgacctcg cccagcaccg ggagaggcac
     4381 gcccgcgagg cccgccgcac cgaggagtcg ctctcgtcct cggagctgca gctcatcgcc
     4441 atcgacatgc gccaggccga gctcaagatc cagcaggccc gccaggcgtt gaccgcgctg
     4501 gccgtcaccg cgccgcacga cggcgtgctg gtgatgaacc gcgactggcg gggcaccccc
     4561 acccgggtgg gggactcggt ctggaacggc cagccgctgg cccagctccc cgacctgtcc
     4621 accatggagg ccgaagtctt cgtgctggag gccgacgccg gcgggttgga ggcgggcaag
     4681 ccggccacgg tggtggtgga gtcggcgccc ggcgtctcct accaggccaa gatccgccgc
     4741 gtcgaggcgc tggccaagcc ccgcctccgc ggctcgccgg tccagtactt cgccgtcacc
     4801 ctggccctgg accgcaccga tccgcgggtg atgaagcccg ggcagcgcgt ccgcgccaac
     4861 ctcctcctcg aggagcggca gggggcgctg acggtccccc gccaggccgt cttccagagc
     4921 gaggggcgga cggtcgtcca ccgcaagaag ggggacggct tcgaggcggt ggaggtgaag
     4981 ctgggctcct ccggcgccgg acgggtggtg gtggagtcgg ggatcggcga gggggacctc
     5041 ctggcgctgg tggatcccgc gcggccggtg ggggaggacg ccacggccga agaggaggcg
     5101 agccccacgc cgggccgtcc gtcggcgccc gtcgctccgg gaggggcgcg ttgaacccgc
     5161 gcgaggcgtt ccgctcagct ctcgagaacc tggccggcca caagctgcgc tcggccctca
     5221 ccatgctggg gatgatcttc ggcgtcggcg cggtcatcgc catgctttcg atcggcgccg
     5281 gcgccgagga gcaggcgctg gggatgatcg agcgcctggg cctgcgcaac gtcctcatcc
     5341 gcgcccggga gtacaagacc gaggagctgc aggagatccg ccagaagtcg ctgggcgtct
     5401 ccttccgcga cgccgccgcc atctccgagg cggtcccggg cgtcgacctg gtggcgccgc
     5461 gcatccgcat cgagccctac aaggtgctgt ccgccgcggg caagaccgag gccacggtct
     5521 tcggcgtcag ccaccaccac gccgggctcg ccgacctgga gctggccgag ggccgcttcc
     5581 tggacgccct ggacgagagc acgcacgccc aggtctgcgt catcggaccg ggggtgcggc
     5641 gggacctctt cggctacggc ccggcggtgg gggaagaggt caaggtggac gacgtctggc
     5701 tggaggtgat cggggtgctc gccgccaccg gccaggggga gaccttcgaa ggggtgccgg
     5761 tgggctcggt ggagaccgag atctacctgc cggtcaccac cgccgaacgc aagttcgagc
     5821 gcccgcccct cgactcgccg ctcgacgaga tcgtggtccg gctgaaagag gggcagagcg
     5881 gccgggaggc ggcggcggtg atcagcggcc tcctgtcgcg cctgcacgcc ggcaccgacg
     5941 actacgagat cgtggtcccc gaggcgctcc tggaacagag ccggcgcacc cagcgcctgt
     6001 tcaatgtggt catgggctgc atcgccggca tctcgctgct ggtcggcggc atcggcatca
     6061 tgaacatcat gctggcgacg gtgctggagc ggacgaggga gatcggcgtc cgccgcgccg
     6121 tgggggcccg ccgcagcgac atccgcttcc agttcgttgt cgagtccttc gccatcagcc
     6181 tgatcggcgg cctcgccggg gtgatcgccg gcatcgccat cgcgaggggc gtggcggtct
     6241 atgccggctg ggagacgatc gtcaccgtcg gctccatcct gatctccacc ggcgtcgcca
     6301 tgaccgtcgg cctcgcctcc ggcatctacc ccgccacccg cgccgccggc ctcgatccca
     6361 tcgaggcgtt gcggtacgag taggacctca tggcccgcac gagtaagctg gagagcctga
     6421 gctcgcggga ctatctcctt acagatcctg aggagttggt ccacatcgac tggcttggcg
     6481 agttgggcgg tggtgcctcc ccccctgggg gacgggagga agggagcggg ggaggccagg
     6541 aggggcggtc ttcttccttc gccgagggaa aagggcaatc tcgctgctcc gcgctggaag
     6601 ctggatggga gggaaagctg ctgccctcac caaagcggcc cctctcctaa cctcccttac
     6661 tccttccacg gaacgatatc ggccttccgg gcccccccac ccttgccggc ccgccctccc
     6721 atgcgggaga atggtccctg cttcctgacg ttcacccggc atgatcgaaa ccctgactca
     6781 acagaagtcc cttccctcca gcgaggagtc ggagagggcg gtcctcggcg gcgtcatgct
     6841 cgacccggcc gtgctgccca ccatttcggg ccggctgcgc cccgaagatt tctacctgga
     6901 acgccaccag atcctctatc aggcgatgat cgacctgcag gaggagctgg tcgacatcga
     6961 cctgcgcacc ctccaggcca agctggagca gcaaggcaag ttcggcaccg tcggcggcct
     7021 ctcctacctg accggactgg acctcgacct cccggacatc gggcggatcg acgcctacgt
     7081 cgagatcgtc aaggagcgct ccgtccgccg gcgcctcatc caggcatcga gccagatcat
     7141 ccgcgactgc ctggacggcg ggctcagtgc ccaggcggcc ctcggccgcg ccgagcaggc
     7201 gatcctcggc ctgggcgagg aggcgatcca gaagggcttc gccctgctgg gcgacgtctt
     7261 ccacaccacc ctcgaggaga tagaggagcg gccggggagc atgctcaccg gcatccccac
     7321 cggctacatc gacctcgacc gcatcaccca ggggctgaac aagggcaacc tgatcatcat
     7381 cgccggccgc cccggcatgg gcaagaccag cttcgccctg aacgtcgccc agcacgtcgc
     7441 gatccgcgag cggcggacgg tgggcatctt ctctctggag atgagccagc aggagctggc
     7501 gctgcgcatc ctctgctccg agtccgacat ctccttcgcc cgcctgcgcg ccggccggat
     7561 ctcgcagaag gagtggacca agatcatgac gaccgtgcgc ggggtgggcg acgcgccgct
     7621 ctacatcgac gactcgccca acccgacgct cctggaggtc gcctcgaagt cgcggcggct
     7681 caaggcggag aagggattgg gggtcctggt cctcgactac ctgcagctca tgcaggccgg
     7741 cggcaagtac gagaaccgca acctcgagat cgccgccatc agccgcggct tgaagcagct
     7801 cgccaaggag ctggagatcc ccgtcatcgc cctctcccag ctctcccgga accccgaccg
     7861 gcgcggcgcc gaccaccggc cgcagctctc cgacctgcgc gagtcgggct caatcgagca
     7921 ggacgccgac atggtggcgt tcatctaccg tgacgaggtc tacaacccca ccgaggagaa
     7981 caaggggctc gccgagctga tcatcgccaa gcaccggaac ggcgagacgg gcaccgtcga
     8041 gatggtattc ctgggcgaga ccaccagctt ccgcaacctc gaccgccacg gcgctcccga
     8101 tgtcccagca ccgttctgac gccctgctga ccaaggggga ggagctcgcc tccgccctgc
     8161 ggccggcctg ggtggacgtg gacctcgatg cgctgggaca caacctccag gtcatccgcc
     8221 gccagacccc cggcacccgg ctcatggctg tggtcaaggc cgacgcctac ggccacggcg
     8281 ccgccgcggt gagccgcgcc ctcgccggcg cgggcgtcga ctggctcggt gtcgccctgc
     8341 tcgaagaggg ggccaagatc cgccgggcgg gcgtcgagac ccccatcctg gtgctgggta
     8401 cggcgcggcc ggccaagatc gccctctacc agcgctaccg gctggtgccc acggtgtcga
     8461 gcctcgccga gctcggcctc tggcgtgact gggtggggac gcgcgccgag ccgcagccca
     8521 tccatctgaa ggtggacacc ggcatgggcc gcctcggcgt cgcggtcgag cgcgcacccg
     8581 agctcgtgcg cgaggcgacg agcagcggca tccaggtcgt cggcctgatg acgcacctgg
     8641 cg
//
LOCUS       DASPJI010000723        12263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_392906, whole genome shotgun sequence.
ACCESSION   DASPJI010000723 DASPJI010000000
VERSION     DASPJI010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12263)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12263
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392906"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VEL74_20265"
     CDS             <1..562
                     /locus_tag="VEL74_20265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64928.1"
                     /translation="GIDLLAWGYRRQLAETVELEGTFYGGDLDLLRGPGNGPILPISG
                     DDKEEVGANVWLYLGGLTFFGQYVDQELAGLPRTGIEGEMAWRFELPLVWTIGEQQLF
                     TFIQPAVRYSKIDNGFENVPRSATAPGTPAPSLAWDWEKIDAGVRAGVIPGVDLTVEY
                     ADNRFILGSGAEEDNNEFLATLRWRM"
     gene            599..1294
                     /locus_tag="VEL74_20270"
     CDS             599..1294
                     /locus_tag="VEL74_20270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64929.1"
                     /translation="MKDRRASSTLLLLWALLSLAGAARADEIRGRVELLAKGGKGPAR
                     GSDVRDVVVYFEPASPQAVKPAAQAFEVVTKDKEFQPRVLAIPRGSRVRFPNQDPILH
                     NVFSVSGVNRFDLGLYRRGAGKEKRFDEAGIVRVFCNVHHDMVAYILVLDTPFSASPA
                     ADGTFVLTGVPRGPGRLTVWHEQAEIWTTEVRLPQSAPVAARVEVTKPRIPAHLNKTG
                     SSYFRRDRYGKDR"
     gene            1335..3935
                     /locus_tag="VEL74_20275"
     CDS             1335..3935
                     /locus_tag="VEL74_20275"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HYG64930.1"
                     /translation="MTSLLIALSVGAAVAATYLLVQRISRGAALESIQSSSSVQSAFR
                     ERDYQQLKLIARLMASDPYLVAYLAEAAENRDAASILDLLTERQKDFGYGFAAVLDAN
                     GRVVARTDRPAAAGQDLSGRALVAKVRTEFEAQGVWREGDELDYAVAVPIAQDFTPVG
                     YLVTGFPLPEAESIAEISGADMAFVTDDAAGPRVVATTLPPGVSRNLAAALRSQEEGM
                     GEAMRAGKVVPELELTLDGEPWIALQTPLTDAAGKPVGASIALASLARPMAAYREIET
                     ALLAVGLAVVLLGPLLAFAFARRALAPMRRLLTATEAARQGDYDQKIAVTGRTDEVGR
                     LANAFDELLADLREKRDMEAYVTELSRNLPEPAQGRAVPGAPQTRDVLLLAIELRRYV
                     RAAASLADGLPQDTLDLLAVDLERITSAITAQRGQVEAIAGHRVVARFEGEGRALRAL
                     SAAARVLGEGDDDPDAEADAPAVAMASGRAVAGPVSWGDRLERALVGLPVQQVEGLLR
                     EATPGEAILSRDVHEELREALEQAGYRLAERRGILSTQPLYAVGGRMATRVVSTRGAA
                     GAAGATGSGAGTETVAPAGAATLSGIAPGALMGQRFEILSVLGVGGMGVVYKARDREL
                     DDLVALKMMKREMWGDRTQLERLKSELKLARKITHPNVLRTHDFGEIDGIPYISMEYV
                     RGVTLRYLLDQQASRLPYSAGLRVAKQLCAGLGAAHSVGVIHRDIKPENLILEPTGNA
                     KLMDFGIARPVVRAAPGQTQAGFIVGTPQYLSPEQLQGREPDTRADIYSCGMVFYEMF
                     TGELPFTGATAVEIAMKHLQVEPEPPSVRWREVPPALDVIILRCLAKDPDARYRSVQD
                     LLRELEALSA"
     gene            3966..5183
                     /locus_tag="VEL74_20280"
     CDS             3966..5183
                     /locus_tag="VEL74_20280"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HYG64931.1"
                     /translation="MIAEMEPLPDPPSPPTPLASMEPLAPGESPGDDLRRRLAEYETM
                     LDIGARLAGTLDLEVVLERALENAETVSRAETSSIWELDEEKQELYFRVVRGRAAGEI
                     RGLRVPVGEGIVGSVARSGLAEVVNDVAADPRWRGDGQAGFRTRALMAVPLTVHGSVI
                     GVLQLLNPVGRERFTEDDLRRMTLLAAILAPPLQNARLYAAQRRQFLRMVTALAESLE
                     KKDPYTGGHVRRVVGYSLLLGAEMGLSREQLKDLWLAATLHDIGKIGVPDRILGKPGP
                     LDPAESEIMKQHTLYGSDIVTHLANSTVLRGVRSHHERIDGNGYPDRLRGPEIPLAPR
                     IIAVADTYDAMTTSRPYRAGLRPERAASEILGGAGTQFCPQVVVAFRNLFESGRFTLE
                     SAEEPLRGVSWVD"
     gene            5240..6919
                     /locus_tag="VEL74_20285"
     CDS             5240..6919
                     /locus_tag="VEL74_20285"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HYG64932.1"
                     /translation="MLQLRFAVDGQDRVFPLAGDWVRLGRGSDNDVVLPDVSVSRYHA
                     ELRREPVGWSVYDLKSTNGVELNRAAVERAVLQAGDRLTIGIFELDVEAAQEAPPPSP
                     PEPLQAPLPTGASVLPADSGAGSGDSLEPLSNATIVRPLSEFVAAYGLESGQTMGVVR
                     EDDSRKDTGDLAYTDRMFGFLTRLARILIVAESVDEVLLKVLDIAFEALPVDRGFILL
                     SGEDGELVCELARVKDRVELRPSAEVPVSRTMLRAVMRERVALVTFDARSDERLSGGA
                     SILIHQIRAAMCTPLWAGDRIIGVVQVDSPFQVGAFGERDLDFLTTLANYVAVAVERI
                     RWARQAERERQVRARLERYHSPAVIEEVLREEGAETQRLKSTVATVLFADLVGFTSFA
                     ESSRPEEVAELIDGFLGLSVEAIFRAGGTLDKFIGDCVMAFFGAPMAQPDHALRATQA
                     AVDIQRGLAAWNAERAAQGLRAFQARVALNSGPVVVGDIGSARRVDYTVLGNTVNVAA
                     RLEAYVARPGDVVVGPETHRLMAGALPTEPLGELQLKGLQQKITAFRVTGV"
     gene            complement(6965..7771)
                     /locus_tag="VEL74_20290"
     CDS             complement(6965..7771)
                     /locus_tag="VEL74_20290"
                     /inference="COORDINATES: protein motif:HMM:NF039204.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HYG64933.1"
                     /translation="MPPKHLPSPSTSRLRSVTGSAIAVLLSLACLGGPSSEAQSLIGS
                     RAALVKAGGEARQHGYTYLRTGSQVREFVNRGLLVRLSGNSSYRVNSVSYPYARPEVK
                     LFVERLSRQYRSACGEKLVVTSLTRPLSRQPANASHLSVHPTGMAMDLRRSSRASCRR
                     WLESTLLKLEDRGVLEATKENHPPHYHVSLFPQRYTTYLASRGDLPRGSSSGTKMAVA
                     SDTTKYRVRGGDSLWEIARRHGTSVSQIKRANGISSSRLKPGQMLQIPAR"
     gene            complement(8012..9409)
                     /locus_tag="VEL74_20295"
     CDS             complement(8012..9409)
                     /locus_tag="VEL74_20295"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HYG64934.1"
                     /translation="MNEQDTQNADFAQALAEFEQQGGGGEGGADQRKNPAVGERVKGQ
                     VVSVTDENVFIDIGAKAEAVMEAAQFKDAEGNLTINAGDSVEATVASIDAETGAAVLR
                     KKAGGGRRGGGQQQQEIAAELRQAYENSLPVEGTVTAVNKGGVEVQVSGMRAFCPHSQ
                     LDLRYVQDTQQFIGQKLTFRITRLEEGRGRPNIILSRRTILEEEAGAKAAEARSRIEV
                     GTVTSGKVTSLTSYGAFVDLGGIEGMLHVSEIGYARLAHPKEALSVGDEVEVQVIKIE
                     KGKDGRDRISLSRRALERDPWKDVAENFTEGTEVAGRVMRLETFGAFVEIAPGIEGLV
                     HISEMGAGRRLQHSREAAEVGQEVRVRVLGVDPDKRRVSLSMGSGSGAERTADRPERG
                     ERGGFGGGERERGRGDRGGSRGGDRGGDRNRGRGGRDRYDDRVAEPVGAPRSISTGSG
                     FGSMADFFKNLKKND"
     gene            9616..>12263
                     /locus_tag="VEL74_20300"
     CDS             9616..>12263
                     /locus_tag="VEL74_20300"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYG64935.1"
                     /translation="MKRSTVALLLIALPFPASVLTPASVSASPGPVPVDPREVHLAEL
                     RQLTFGGENAEAYWSPDGTQLVFQSTRPPYACDQIFRVPAAGGASPEASPVRVSTGQG
                     RTTCPYFTADGRRILFSSTHHAASACPPPPDRSQGYVWPLDPSYEIWSAEPDGSGLAR
                     LTDNDAYDAETTVCPLDGSLLFTSTRDGDLELYRARADGGEVRRLTHAPGYDGGAFFS
                     PDCAKIVWRASRPKEGEELDDYRRLLARGLVRPSRLEIWVADADGGNARQVTSLGAAS
                     FAPSFFPSGDRIIFSSNHGDPKGREFDLWAVNVDGTGLERITHTPGFDGFPLFSPDGT
                     RLAFSSNRVQAAEGETNVFVARWAEGSSNAKSAASGPERYTRYENEVRWLADDARGGR
                     GVGTPGLAEAERWLVERFREIGLEPGGDPPGPQGPQGRGGPYVHGFDVPVSVEVKPGT
                     RVAVDGKPLAAEDFQPTAFSSSGTVEGEVVAAGYGITAPELGVDDYAGIDAKGKIVVV
                     RRFVPAGGSGLPFAQREAERRYSELRYKAWNAREHGAVALLVVDLPERAGDPPREAAF
                     PTLAGTTEGDAGIPVVVLGRSVGAPLFAGGHRVSLTVDLARRLEPAGNVVGVLRAGAA
                     DRLPGAVLVGAHYDHLGMGGHGSLAPGVTETHNGADDNASGTAALLEVARQLAAKRSE
                     LRRDVWFVAFSGEEMGVLGSTAFTRRLSNGAGGASGALKMTDLVAMLNMDMVGRLRES
                     RLTVFGTDSAEEWGGLVTSLCQEAGLACTQTGDGYGPSDHSPFYGAGVPVLHFFTGTH
                     DDYHRPSDDADKVSAEGGVRVAGLVARVAEELAGRPARLTYKNAPAPGPRGDMRPTGA
                     SLGLVPDYAGGGEPGVLISGMRPGSPA"
BASE COUNT         1666 a         4398 c         4436 g         1763 t
ORIGIN      
        1 cggcatcgac ctcctcgcct ggggctaccg gcgccagctc gccgagaccg tggagctcga
       61 agggacgttc tacggcggcg acctcgacct gctgcgcggg cccggcaacg gcccgatcct
      121 gcccatctcc ggcgacgaca aggaagaggt gggggcgaac gtctggctct acctcggcgg
      181 cctgaccttc ttcggccagt acgtggacca ggagctggcc ggcctgccgc gcaccggcat
      241 cgagggggag atggcctggc gcttcgagct gcccctggtc tggaccatcg gcgagcagca
      301 gctcttcacc ttcatccagc cggcggtgcg ctactcgaag atcgacaacg gcttcgagaa
      361 cgtcccgcgg tcggccaccg cgcccgggac gccggcgccg agcctggcct gggactggga
      421 gaagatcgac gccggcgtgc gcgccggcgt catccccggg gtcgatctca cggtagagta
      481 cgcggacaac cgtttcatcc tcggctccgg cgccgaagag gacaacaacg agttcctggc
      541 cacgctgcgc tggcggatgt gaccctgccg gccggttcgg ccaggggagg cgtatccaat
      601 gaaggaccga cgtgcatcgt caaccctgct cctcctgtgg gcgctgctgt cgctggccgg
      661 cgccgcgcgg gcggacgaga tccgtgggcg cgtcgagctc ctcgccaagg ggggcaaggg
      721 gccggccagg gggagcgacg tgcgcgatgt cgtcgtctac ttcgagccgg cgagcccgca
      781 ggcagtcaag ccggcggcgc aggcgttcga ggtcgtcact aaggacaagg agttccagcc
      841 gcgggtgctc gccatcccgc gcggcagccg ggtgcgcttc ccgaaccagg acccgatcct
      901 ccacaacgtc ttctcggtct ccggcgtcaa ccgcttcgac ctcgggctct accggcgcgg
      961 ggcgggcaag gagaagcgct tcgacgaggc cggcatcgtg cgggtgttct gcaacgtcca
     1021 ccatgacatg gtggcctaca tcctggtgct ggacaccccc ttctccgcct cgccggcggc
     1081 cgacggcacc ttcgtgctga ccggcgtgcc gcgcgggcca ggccgcctca ccgtctggca
     1141 cgagcaggcc gagatctgga ccaccgaggt ccggctgccg cagagcgcgc cggtggccgc
     1201 ccgcgtcgag gtcaccaagc cgcgcatccc cgcccacctc aacaagaccg gctcctccta
     1261 cttccggcgt gaccgatacg gcaaggaccg gtagccgacg cctcggcctg tcgctcgagg
     1321 ccaggatctt cctggtgacc tcgctgctca tcgccctctc ggtaggcgcg gcggtggcgg
     1381 cgacctacct gctggtgcag cggatctccc gcggcgccgc cctggagtcg atccagagca
     1441 gctcttcggt gcagtcggcg ttccgcgagc gcgactacca gcagctcaag ctgatcgccc
     1501 gcctcatggc ctccgacccc tatctggtgg cctacctcgc cgaggcggcg gagaaccggg
     1561 acgccgcctc gatcctcgac ctgctcaccg agcgccagaa ggacttcggc tatggcttcg
     1621 ccgccgtcct cgacgccaac ggccgggtgg tggcccgtac cgaccggccg gcggccgcgg
     1681 gacaggacct gtcgggccgg gcgctggtcg ccaaggtgcg gaccgagttc gaggcgcagg
     1741 gtgtctggcg cgagggggac gagctcgact acgccgtggc cgtgccgatc gcccaggact
     1801 tcacccccgt ggggtacctg gtcaccggct tcccgctgcc cgaggccgag tcgatcgccg
     1861 agatcagcgg cgccgacatg gccttcgtca ccgacgacgc ggcgggcccc cgggtagtcg
     1921 ccaccacgct gccgccgggc gtgtcgcgca acctcgcggc ggcgctacgc tcgcaagagg
     1981 aggggatggg cgaggcgatg cgcgccggca aggtggtccc cgagctggag ctgaccctcg
     2041 acggcgagcc gtggattgcg ctccagacgc cgctcaccga cgccgccggc aagccggtcg
     2101 gcgcgagcat cgccctcgcc tccctcgccc ggcccatggc ggcctaccgg gagatcgaga
     2161 ccgcgctcct cgccgtcggg ctcgccgtgg tgctcctggg gccgctcctc gccttcgcct
     2221 tcgcccgccg cgccctcgcc cccatgcgcc gcctgctcac ggcgaccgag gcggcgcgcc
     2281 agggggacta cgaccagaag atcgccgtca ccggccgcac cgacgaggtc ggccggctcg
     2341 ccaacgcctt cgacgagctg ctggccgacc tgcgcgagaa gcgggacatg gaggcctacg
     2401 tcaccgagct ctcgcgcaac ctccccgagc ccgcccaagg gcgcgccgtg cccggggcgc
     2461 cgcagacccg tgacgtgctg ctcctcgcga tcgagctgcg gcgctacgtc cgggcggcgg
     2521 cgagcctggc ggacggcctg ccccaggaca ccctggacct cctggccgtg gacctcgagc
     2581 ggatcacctc ggcgatcacc gcgcaacgcg gccaggtaga ggccatcgcc ggccaccggg
     2641 tggtggcccg cttcgagggg gaggggcggg ccctgcgcgc gctgtccgcc gccgcccgcg
     2701 tgctggggga gggggacgac gatcccgacg ccgaggcgga cgcgccggcg gtcgcgatgg
     2761 cgtcaggacg cgcggtggcc gggccggtct cctgggggga ccgcctggag cgggcactgg
     2821 tagggctgcc ggtccagcag gtggaggggc tgctgcgcga ggcgacgccc ggcgaggcga
     2881 tcctctcgcg cgacgtccac gaggagctgc gcgaggcgct ggagcaggcc ggctaccggc
     2941 tggccgagcg ccggggcatc ctgagcaccc aaccgctcta tgcggtgggc ggccgcatgg
     3001 cgacccgggt ggtgagcacc cgcggcgccg ctggcgccgc gggcgccact ggctccggcg
     3061 ccggcaccga gacggtggcg ccggccggcg cggcgacgct gtcggggatc gcgccgggcg
     3121 ctctcatggg gcagcgcttc gagatcctct cggtcctggg cgtcggcggc atgggcgtgg
     3181 tctacaaggc gcgcgaccgc gagctcgacg atctggtggc gctcaagatg atgaagcggg
     3241 agatgtgggg cgaccgcacc cagctcgagc gcctcaagag cgagctcaag ctggcaagga
     3301 agatcaccca tccgaacgtc ctgcgcaccc acgacttcgg cgagatcgac ggcattccgt
     3361 acatctcgat ggagtacgtg cgcggcgtca ccctccgcta cctgctcgac cagcaggcga
     3421 gccgcctccc ctactcggcg gggctgcgcg tggccaagca gctctgcgcc ggcctgggcg
     3481 ccgcgcactc cgtcggcgtg atccaccgcg acatcaagcc cgagaacctg atcctggagc
     3541 ccaccggcaa cgccaagctg atggacttcg gcatcgcccg cccggtggtg cgggcggcgc
     3601 cggggcagac gcaggccggc ttcatcgtcg gcactccgca gtacctgtcg cccgagcagc
     3661 tccaggggcg cgagcccgac acccgcgccg acatctactc ctgcggcatg gtcttctacg
     3721 agatgttcac cggcgagctg cccttcaccg gcgccaccgc cgtggagatc gccatgaagc
     3781 acctgcaggt ggagccggag ccgccgagcg tccgctggcg cgaggtgccg ccggcgctcg
     3841 acgtcatcat cctgcgctgc ctggccaaag acccggacgc ccgttaccgg tcggtccagg
     3901 acctgctccg ggagctggag gcgctgagcg cctgaacggg gcctgaacaa ggggaaaacg
     3961 agcccatgat cgccgagatg gaaccgctgc ccgatccgcc gagccctccc acacccctgg
     4021 cttccatgga gcccctggcg ccgggtgaat cgccgggtga cgacctgcgg cgccggctgg
     4081 ccgagtacga gaccatgctc gacatcggcg cgcggctggc cggaacgctg gacctggagg
     4141 tggtgctcga gcgggccctc gagaacgccg agacggtcag ccgagccgag accagctcga
     4201 tctgggagct ggacgaggag aagcaggagc tgtatttccg ggtggtgcgc gggcgggccg
     4261 cgggggagat ccgcggcctc agagtgccgg tgggggaggg gatcgtcggc agcgtcgccc
     4321 gctccgggct cgccgaggtg gtcaacgacg tggccgccga cccgcgttgg cgcggcgacg
     4381 gccaggccgg gttccgcacc cgggcgctga tggccgtgcc gctgaccgtc cacggcagcg
     4441 tcatcggcgt gcttcagctc ctcaacccgg tggggcgcga gcgcttcacc gaggacgacc
     4501 tgcggcggat gaccctgctc gccgccatcc tcgcgccgcc gctgcagaac gcccgcctct
     4561 acgcggcgca gcgccggcag ttcctgcgca tggtgaccgc cctcgccgag tcgctggaga
     4621 agaaggatcc ctataccggc ggccacgtgc gccgggtggt cggctattcg ctcctgctgg
     4681 gtgccgagat ggggctctcg cgcgagcagc tcaaggacct ctggctcgcc gccacgctcc
     4741 acgacatcgg caagatcggc gtgccggacc gcatcctcgg caaacccggg ccgctcgacc
     4801 cggccgagtc ggagatcatg aagcagcaca ccctctacgg ctcggacatc gtcacccacc
     4861 tggcgaactc gaccgtgctg cgcggcgtgc gcagccatca cgagcggatc gacggcaacg
     4921 gctaccccga ccggctgcgc gggccggaga tcccgctggc gccgcgcatc atcgccgtgg
     4981 ccgacaccta cgacgcgatg accaccagcc gcccctaccg cgccgggctg cggccggagc
     5041 gcgccgcctc cgagatcctg ggcggcgccg gcacccagtt ctgcccgcag gtggtggtgg
     5101 ccttccgcaa cctcttcgag agcgggcgct tcacactgga gtcggccgag gagccgctgc
     5161 gcggcgtctc ctgggtggac tagcgggggc gccccgcagg gggcccctgc tgctatgatc
     5221 ccggcgaggc ctcctcttca tgttgcagct ccgcttcgcc gtcgacggcc aggaccgtgt
     5281 cttccccttg gcgggggact gggtgcggct cgggcgcggg agcgacaacg acgtggtcct
     5341 gccggacgtc tcggtgtcac gctaccacgc cgagctgcgg cgcgagccgg tgggctggtc
     5401 ggtctacgat ctcaagagca ctaacggcgt cgagctgaac cgcgccgcgg tggagcgcgc
     5461 ggtgctccag gcgggcgacc gcctgacgat cggcatcttc gagctggatg tggaggccgc
     5521 gcaagaggcg ccacccccgt ctccccccga gccgctccag gccccgctcc ccacgggcgc
     5581 ctcggtcctg ccggccgatt ccggggctgg cagcggtgac agcctcgagc cgctgagcaa
     5641 cgccaccatc gtccggccgc tctccgagtt cgtcgccgcg tatggcctgg aatcgggcca
     5701 gacgatgggc gtggtccgcg aggacgatag ccgcaaggac acgggcgacc tcgcctacac
     5761 cgaccggatg ttcggcttcc tgacgcgcct cgcgcgcatc ctgatcgtcg ccgagtcggt
     5821 ggacgaggtg ctgctcaagg tgttggacat cgccttcgag gcgctgccgg tggaccgcgg
     5881 cttcatcctg ctgtcgggcg aggacggcga gctggtctgc gagctggcgc gggtcaagga
     5941 ccgggtggag ctgcgcccct cggccgaggt gccggtgtcg cggaccatgc tgcgcgccgt
     6001 gatgcgggag cgggtggcgc tggtgacgtt cgacgcccgg tcggacgagc ggctgtcggg
     6061 cggcgcctct atcctgatcc accagatccg cgccgccatg tgcacgccgc tctgggccgg
     6121 cgaccggatc atcggcgtcg tgcaggtgga ctcgccgttc caggtggggg cgttcggcga
     6181 gcgcgacctg gacttcctga cgacgctcgc caactacgtg gcggtggcgg tggagcggat
     6241 ccgctgggcg cggcaggccg agcgggagcg gcaggtgcgg gcgcgcctgg agcgctacca
     6301 ctcgccggcg gtcatcgagg aggtgctgcg cgaggagggg gccgagacgc agcggctgaa
     6361 gagcaccgtg gcgacggtgc tcttcgccga cctggtgggc ttcacctcct tcgccgagag
     6421 ctcgcggccg gaggaggtgg cggagctcat cgacggcttc ctggggctgt cggtggaggc
     6481 catcttccgg gccggcggca cactcgacaa gttcatcggc gactgcgtca tggctttctt
     6541 cggcgctccc atggcgcagc cggaccacgc gctacgggcg acgcaggccg cggtggacat
     6601 ccagcgcggc ctcgcggcct ggaacgccga gcgcgcggcg caggggctgc gcgcctttca
     6661 ggcgcgggtg gcgctgaaca gcggccccgt ggtggtgggg gacatcggca gcgcccgccg
     6721 ggtggactac accgtgctgg gcaacacggt gaacgtcgcc gcccgcctgg aggcctacgt
     6781 cgccaggccc ggtgacgtgg tcgtcggccc cgagacccat cgcctcatgg cgggcgccct
     6841 ccccaccgag ccgctgggcg agctccagct caaaggtctg caacagaaga tcacggcatt
     6901 tcgggtgacg ggggtgtaga gggcgggagg ggcaggtgcc cctcccgggg aagccgaagc
     6961 gcgctcagcg cgccgggatc tgcagcatct ggccgggctt gagccggctg gagctgatgc
     7021 cgttggcgcg cttgatctgc gagacgctcg tgccgtgacg gcgggcgatc tcccagaggc
     7081 tgtcgccgcc ccgcacccgg tacttcgtgg tgtcggaggc cacggccatc ttggtcccgg
     7141 agctgctgcc gcgcggcagg tcgccccggc tcgcgagata ggtcgtgtag cgctgcggga
     7201 agagcgacac gtgatagtgc ggagggtggt tctccttggt tgcctcgagc acgccgcggt
     7261 cctcgagctt gagcagcgtc gactccagcc agcggcggca ggaggcgcgc gagctgcggc
     7321 gcaggtccat ggccatgccg gtggggtgca ccgacaggtg cgaggcgttg gccggctgcc
     7381 gcgacagcgg gcgcgtcagg ctggtgacca ccagcttctc gccgcaggcg ctgcggtact
     7441 ggcgggagag ccgctcgacg aacagcttca cctccggccg cgcgtaggga taggagaccg
     7501 aattgacccg gtagctggaa ttgcccgaaa ggcgcaccag cagcccccgg ttgacgaact
     7561 cgcgcacctg ggagccggtg cgcagatagg tgtaaccgtg ctgcctggct tcaccgccgg
     7621 ctttgaccag cgcggcccgc gagccgatca ggctctgggc ctcggagctc ggtccgccga
     7681 ggcaggcgag ggacaggagg acggcgatcg cgcttccggt gacggagcgc agacgcgagg
     7741 tggacgggct cggaagatgc ttgggcggca ttccatactc catgaccagc gaggggccca
     7801 atgagccttg gctctgcgaa aacggtgatc cacttggcgt gtgtagggta gcacaaagag
     7861 gccgtttcca gcaccacctt ttttcagtct gtgacctgag gctccgctgc tccccgcgag
     7921 aggaggtggg ggtggagagg taaaaaggaa aaagtcgctc cgcgtgcggg caccggccga
     7981 ggagtggcgc ccgcacgcga acggcagcgg ctcaatcgtt cttcttcagg ttcttgaaga
     8041 agtcggccat cgagccgaaa ccggagccgg tggagatcga ccgcggcgcg cctacgggct
     8101 cggcgacacg gtcgtcatag cggtcccgcc cgccacgccc acggttgcgg tccccgccgc
     8161 ggtccccgcc ccggctgcca ccccggtcgc cgcggccacg ttcacgctcg ccgccgccga
     8221 agccgccccg ctcaccccgc tcgggacggt cggccgtccg ctcggcgccg ctaccgctgc
     8281 ccatggagag ggagacgcgg cgcttgtcgg gatcgacccc gagcacccgc acccgcacct
     8341 cctggccgac ctccgccgcc tcgcgcgagt gctggagccg ccggccggcg cccatctcgc
     8401 tgatgtggac cagcccctcg atgcccgggg cgatctcgac gaaggcgccg aaggtctcca
     8461 ggcgcatcac ccggccggcg acctccgtcc cctccgtgaa gttctcggcc acgtccttcc
     8521 aggggtcgcg ctccagggcc cgccgcgaca gcgagatgcg gtcgcgccca tccttgccct
     8581 tctcgatttt gatgacctgg acctcgacct cgtcccctac cgacagcgcc tccttggggt
     8641 gggcgaggcg ggcgtagccg atctcgctca cgtggagcat gccctcgatg ccgccgaggt
     8701 cgacgaaggc gccgtagctg gtgagcgagg tcaccttgcc gctggtcacc gtgcccactt
     8761 cgatgcggga acgggcctcc gccgccttcg ccccggcctc ctcctcgagg atggtccggc
     8821 gcgacaggat gatgttcggc cggccgcggc cctcctccag gcgggtgatg cggaacgtca
     8881 gcttctggcc gatgaactgc tgcgtgtcct ggacgtagcg caggtcgagc tgggagtgcg
     8941 gacagaaggc gcgcatgccc gacacctgga cctccacgcc gcccttgttg accgcggtca
     9001 ccgtgccctc gaccggcagg ctgttctcgt acgcctgccg cagctcggcg gcgatctcct
     9061 gctgctgctg gccgccgccg cgccggccgc cgccggcctt cttgcgcagc accgcggcgc
     9121 cggtctcggc gtcgatcgag gccaccgtcg cctcgacgct gtcgccggcg ttgatcgtca
     9181 ggttgccctc ggcatccttg aactgggcgg cctccatgac ggcctcggcc ttggcgccga
     9241 tgtcgatgaa gacgttctcg tcggtcaccg agaccacctg ccccttcacc ctctcgccca
     9301 cggcgggatt cttgcgctgg tccgcgccgc cctccccgcc gcctccctgc tgttcgaatt
     9361 ccgccaatgc ctgcgcgaaa tccgcgttct gagtgtcttg ttcgttcatg tgtggttgtt
     9421 agctcctgcc ctcccgggcg ctccgcggtc ctgctcgcct gatggcccct ctgctctggc
     9481 ggggccggta aaatatcacg ccggcatgga tctcacgcgg gtagaatcgg gtggacgatc
     9541 cagactgaga ccagatgcgc cccagagggc aacaaggccc ccgggcgatt tatgccagga
     9601 aagaccgctg attcaatgaa gagatcgacc gttgctctgc tcctgatagc cctgccgttt
     9661 ccggcgtccg tcctgacgcc cgcctcggtg tccgcctcgc cggggcccgt gcccgtcgac
     9721 ccgcgggagg tccatctcgc cgagctgcgc cagctcacct tcggcggcga gaacgccgaa
     9781 gcctactggt ccccagatgg tacgcaactc gtgttccagt ccacccgccc gccctacgcc
     9841 tgcgaccaga tcttccgcgt gccggccgcc ggcggggcgt ccccggaagc cagcccggtg
     9901 cgggtctcga ccggccaggg gcgcaccacc tgcccctact tcacggccga cggccggcgg
     9961 atcctcttct cgtcgaccca ccacgccgcg tccgcctgcc cgccgccgcc cgaccgctcc
    10021 caggggtacg tctggccgct cgatccgtcc tacgagatct ggagcgccga gccggacggc
    10081 tcgggcctcg cgcggctgac ggacaacgac gcctacgatg ccgagaccac ggtctgtccg
    10141 ctggacggct cgctcctgtt cacctcgacg cgggacggag acctggaact ctatcgtgcc
    10201 cgggccgacg gtggcgaggt gcggcggctg acccacgctc cgggctacga cggcggcgcc
    10261 ttcttctccc ccgactgcgc gaagatcgtc tggcgcgcct cccggccgaa agagggggag
    10321 gagctggacg actatcgccg cctcctcgcc cgcggcctcg tccgcccgag ccgcctcgag
    10381 atctgggtcg ccgacgccga cggcggcaac gcccggcagg tgacgagcct gggggcggcc
    10441 tccttcgcgc cgtccttctt cccctcgggg gaccgcatca tcttctcctc caaccacggc
    10501 gaccccaagg gacgggagtt cgacctctgg gcggtgaacg tcgacggcac cggcctggag
    10561 cggatcaccc acaccccggg tttcgacggc ttccccctct tctcgcccga cgggacccgc
    10621 ctggccttct cgtccaaccg cgtgcaggcg gcggaggggg agaccaacgt cttcgtcgcc
    10681 cgctgggccg aaggctcctc caatgccaag agtgccgcga gcggcccgga acgctacacc
    10741 cgctacgaaa acgaggtgcg ctggctggcg gacgatgccc gcggcggccg gggggtgggc
    10801 acgccggggc tcgccgaggc ggagcgctgg ctggtggagc gcttccgcga gatcggcctc
    10861 gagccgggcg gtgatccgcc ggggccgcag gggccgcagg ggcgcggcgg tccctacgtc
    10921 catggcttcg acgtgccggt gtcggtggag gtgaagcccg gcacgcgggt ggcggtggac
    10981 ggcaagcccc tggcggcaga ggacttccag cccacggcct tctcctcctc ggggacggtc
    11041 gagggggagg tggtggccgc cggctacggc atcaccgcgc ccgagctcgg ggtggacgac
    11101 tacgccggca tcgacgccaa gggcaagatc gtggtggtcc gccgcttcgt ccctgccggg
    11161 gggagcggct tgccgttcgc ccagcgggag gcggagcggc gctacagcga gctgcgctac
    11221 aaggcctgga acgcccgtga gcatggcgcc gtcgcgctgc tcgtcgtgga cctccccgaa
    11281 agggcgggcg atccgccgcg cgaggcggcg ttcccgaccc tcgccggcac gacggagggg
    11341 gacgcgggca tcccggtagt cgtcctcggc cggtcggtcg gagcgccgct cttcgcgggc
    11401 gggcaccggg tgtcgctgac ggtggatctg gcgcggcggc tggagccggc cggcaacgtc
    11461 gtgggggtcc tgcgggccgg cgcggcggac cgcctgcccg gcgccgtgct ggtgggggcg
    11521 cactacgacc acctcgggat gggcgggcat ggctcgctgg cgccgggggt gaccgagacg
    11581 cacaacggcg ccgacgacaa cgcctcgggc accgcggcgc tgctggaggt ggcgcgccag
    11641 ctcgcggcga agcggagcga gctgcggcgc gacgtctggt tcgtcgcctt ctccggcgag
    11701 gagatggggg tgctgggctc caccgccttc acccgccggt tgtccaacgg ggccggcggg
    11761 gcgagcgggg cgttgaagat gacggacctc gtcgcgatgc tcaacatgga catggtgggc
    11821 cgactgcggg agagcaggct caccgtcttc ggcaccgatt cggcggagga gtggggcggc
    11881 ctggtgacct ccctctgcca ggaggcgggc ctcgcctgca cccagaccgg cgacggctat
    11941 gggccttcgg atcacagccc cttctacggc gcgggcgtgc cggtgttgca tttcttcacc
    12001 ggcacccacg acgactatca ccggccgtcg gacgacgcgg acaaggtcag cgccgagggc
    12061 ggggtgcggg tggccggcct ggtggcgcgg gtggcggagg agctggcggg acgtccggcg
    12121 cggctcacct acaagaacgc gccggcgccg gggccgcggg gggacatgcg gcccaccggc
    12181 gcctcgctgg gcctggtgcc cgactacgcc ggcggcggcg agccgggcgt cctgatctcc
    12241 ggcatgcggc cggggagccc ggc
//
LOCUS       DASPJI010000724         4204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_850595, whole genome shotgun sequence.
ACCESSION   DASPJI010000724 DASPJI010000000
VERSION     DASPJI010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850595"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="VEL74_20305"
     CDS             complement(<1..1634)
                     /locus_tag="VEL74_20305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64936.1"
                     /translation="MPEVRSTPVVRKSGLLHHARGAGKGRARTGLLALTAAAALLVPT
                     VPARSASTPNGQWVTPQGSSAGTANGDYLSSNSPDGLDEPYRYFVEVPPGLSRLVIEL
                     FDADLGAGGGTEDTAGRDRDRAGGFDGEATYTLVHPSGATRNVRFTTGNATGPTGADN
                     SWLALFDSTGDTVRDEFGAVSYANNDGAVDWAGNWTEVSDNSNPASGDLMISGGELLI
                     QDGANYIYRQADLAGDGFSTATLTFDFRTVNVESSDTVRVDVSGNGGSSWVTLDTFAG
                     AGSGSRSYNISAYIAANTRIRFIRTAGYSGNDEFRIDDVQIRETSIDAGHWELRVDQS
                     DGGGDEINALGIRAHDGTSGAGGTELNVYYDSHNQYGVNPTGSASGANSRSYDYYPYI
                     TSGCTAAKNDFDYDSNSGNAGSQVFRSRTGAFTQTFTSGSLSSNDSWRRDTINRWTTD
                     TASTDYGVWSLDATINTYFTPAVNGNYANLYVSNFQAAANPPAANPTANAFRVYLPTD
                     AGAAPVKPYLEQLLTHKSGTNPVLAGQTSRWQVTIRL"
     gene            complement(1794..3197)
                     /locus_tag="VEL74_20310"
     CDS             complement(1794..3197)
                     /locus_tag="VEL74_20310"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HYG64937.1"
                     /translation="MSPLRGEAPSRRLLRLAGDGAAAMAAFTAAFLVRIHLPIPFTQG
                     LLPSDRLLFLAREWPAVLAAQLVSLYVLGFYDPPRPRSRPEVARRLIGVVALQGLILM
                     GYYFLANRTFPRSVLLLHVLFDFLLLLAFRLILDATERRRERRVALVGAGEAAHEIAE
                     ALRLHPWHGLRVVGFVPLPGEAAPAAADDPVLGPCLGTPEDLPALLAAGRIEDIILAG
                     SGDSWQSRLIDSLAGARPDHTNVLLLPGPFESLIGRMRYRWVHDLPLVEVVRETEWRV
                     NWPVKRLLDLVVGSLLLLAALPVLGACALAVRLTSPGPVLYRQTRVGRGRRPFTLWKL
                     RTMRVDAESGESGEVLAQPGDPRLTPIGALLRRYRLDEIPQLWNVLRGSMSLVGPRPE
                     RPGFVQRYLEEVPGYAERFSLAPGLTGLAQVNGDYHSSPRNKLRYELAYLANWSLWLD
                     VTILLRTVKIVLTSRGV"
     gene            complement(3220..>4204)
                     /locus_tag="VEL74_20315"
     CDS             complement(3220..>4204)
                     /locus_tag="VEL74_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64938.1"
                     /translation="HEARWARLEGTRGFRLLNSFWKAAARGRGRDEAVIAGCVTENTP
                     AMLGETLRLVQSTRWFGGDLARARFIVCAVEAIDPEARRELEGYGAEVRVVQRFDPRN
                     PTANKLRFLEEATRTARDSDLILLLDCDMVVVRDPLPFLQRGALAAKIADVPSVPHEA
                     FERLFEHFGLPLLPRTYLTTLLGAPTMAYFNTAVVAFPGDLARAIVPVWVDYNRRIVD
                     VRDRLMGSCAHHSHQASLTLALASHPIPVAEAPVEINFPLHLTHIEPPQRFLDADPAI
                     LHYHKLVDAEGYLLPSVYPRAQARIEEFNRRLRQERAGAGKRGPVSGRVPV"
BASE COUNT          598 a         1387 c         1608 g          611 t
ORIGIN      
        1 agccggatcg tcacctgcca gcgcgaggtc tggcccgcca gcaccgggtt cgtccccgac
       61 ttgtgggtga ggagctgctc caggtagggc ttgaccggcg cggcgccggc gtcggtcggc
      121 aggtagaccc ggaaggcgtt ggccgtcggg ttcgccgccg gcgggttggc cgccgcctgg
      181 aaattcgaga cgtacaggtt ggcgtagttg ccgttgaccg ccggggtgaa gtacgtgttg
      241 atggtggcgt cgaggctcca gacgccgtag tcggtggacg cggtgtccgt cgtccagcgg
      301 ttgatggtgt cgcgccgcca ggagtcgttc gaggagagcg agccgctggt gaacgtttga
      361 gtgaaggcgc cggtccggct tctgaagacc tggctgccgg cgttgccgct gttgctgtcg
      421 tagtcgaagt cgttcttggc ggcggtgcag cccgaggtga tgtagggata gtagtcgtag
      481 ctccggctgt tggcgccgct ggcgctcccc gtcgggttga ccccgtactg gttgtgggag
      541 tcgtagtaga cgttgagctc ggtgcccccg gcgccggagg tgccgtcgtg agcccggatg
      601 cccagggcgt tgatctcgtc gccgccgccg tcggactggt cgacgcgcag ctcccaatgg
      661 ccggcgtcga tcgaggtctc gcggatctgg acgtcgtcga tccggaactc gtcattgccc
      721 gagtagccgg cggtccggat gaagcggatg cgggtgttgg cggcgatgta ggcgctgatg
      781 ttgtagctgc gtgagccgct cccggcccca gcgaaggtgt ccagggtcac ccaggaggag
      841 ccgccgttgc cggagacatc gacccggacc gtgtcgctgc tctcgacgtt caccgtcctg
      901 aaatcgaagg tcagggtggc ggtggagaag ccgtcacccg cgaggtcggc ctgccggtag
      961 atgtagttgg cgccgtcctg aatgagcaac tcgccgccgc tgatcatcag gtcgccgctc
     1021 gcggggttgc tgttgtcgct cacctcggtc cagttgccgg cccagtccac cgcgccgtcg
     1081 ttgttggcgt acgagacggc gccgaactcg tcgcgtacgg tgtcgccggt ggagtcgaag
     1141 agggcgagcc aggagttgtc cgccccggtg gggcccgtgg cgttgcccgt cgtgaagcgg
     1201 acgttgcgag tggcgccgct cgggtggacc agggtgtagg tcgcctcgcc gtcgaagccg
     1261 ccggcgcggt cacggtcgcg cccggcggtg tcctcggtgc ccccgccggc gccgaggtcg
     1321 gcgtcgaaca gctcgatcac cagacggctc aatcccggcg ggacctcgac gaagtagcgg
     1381 tagggctcgt cgagcccgtc cggcgagttc gaggagaggt agtcgccgtt ggcggtgccg
     1441 gcgctgctcc cctgcggggt gacccactgc ccattggggg tggaggccga ccgggccggg
     1501 acggtgggga cgaggagcgc cgcggcggcg gtcagcgcga gcagcccggt gcgggcgcgc
     1561 cccttcccgg ctccccgggc atgatgaagg agaccgctct tccggacgac aggcgtgctg
     1621 cggacttctg gcatgactcc catgctccct tccgggccga gacccgccgt gcgatgcgaa
     1681 ccggcgtccg tcctcgccga agaatggagg aattgacaca cgacaataat cccaactcgc
     1741 tttaaagtca aggatttcgg tgctgtctta cctacggtta ccgccttccg gcgtcacacc
     1801 ccgcgcgagg tgaggacgat cttgacggtg cggagcagga tggtgacgtc gagccagagg
     1861 gaccagttgg ccaggtaggc cagctcgtag cgcagcttgt tgcgcgggct cgagtggtag
     1921 tcgccgttga cctgggcgag gccggtgagg ccgggggcga gggagaagcg ctcggcgtag
     1981 cccggcacct cctcgaggta gcgctggacg aagcccggcc gctccggccg cgggcctacc
     2041 aggctcatgc tcccccggag cacgttccag agctgcggga tctcgtcgag ccggtagcgg
     2101 cggaggaggg cgccgatggg ggtgagccgg ggatcgccgg gctgcgccag cacctccccc
     2161 gattcgcccg actcggcgtc cacccgcatg gtgcggagct tccacagggt gaacggccgc
     2221 cgcccccggc cgacgcgggt ctggcgatag agcaccgggc cgggggaggt caggcgcacc
     2281 gccagcgcgc aggcgccgag caccggcagc gcggcgagga gcagcagcga ccccaccacc
     2341 aggtcgagca gccgcttgac cggccagttg acccgccact ccgtctcccg caccacctcg
     2401 acgagcggca ggtcgtgcac ccagcgatag cgcatgcggc cgatcaggct ctcgaacggg
     2461 ccggggagca gcagcacgtt ggtgtggtcc gggcgggcgc cggcgaggct gtcgatcagc
     2521 cggctctgcc agctgtcgcc gctgccggcg aggatgatgt cctcgatccg gccggcggcg
     2581 agcagcgccg gcaggtcctc cggcgttccg aggcaggggc cgagcaccgg gtcgtccgcc
     2641 gccgccggag ccgcctcgcc cggcaggggt acgaagccca ccacccgcag gccgtgccag
     2701 gggtggagcc gcagcgcctc ggcgatctcg tgcgccgcct cgccggctcc caccagggcc
     2761 acccgccgct cgcgccgccg ctcggtggcg tcgaggatca gcctgaaggc gaggagcagc
     2821 aggaagtcga acaggacgtg gagcagcagg accgagcgcg ggaaggtccg gttggccagg
     2881 aagtagtacc ccatgaggat cagcccctgg agcgcgacca cgccgatcag gcggcgggcg
     2941 acctccgggc ggctgcgcgg ccgcggcggg tcgtagaagc cgagcacgta gagggagacg
     3001 agttgggcgg cgagcaccgc cggccactcg cgggcgagga acagcagccg gtcgctcggc
     3061 agcagcccct gggtgaacgg gatcggcagg tggatgcgga ccaggaacgc cgcggtgaag
     3121 gcggccatgg ccgcggcgcc gtcgcccgcc agccgcagca ggcgccggga gggggcctcg
     3181 ccccgcaggg ggctcatcgc cggccgggct cctcggattt cacaccggca ctctacccga
     3241 aaccggcccc cgcttccctg cgccggcccg ctcctgccgc aggcgccggt tgaactcctc
     3301 gatccgcgcc tgggcccgcg ggtagacgga ggggaggagg tagccctcgg cgtccaccag
     3361 cttgtggtag tggaggatgg cggggtcggc gtccaggaag cgctgcggcg gctcgatgtg
     3421 ggtgaggtgg agcgggaagt tgatttccac cggcgcctcg gccaccggta tcgggtggga
     3481 cgcgagcgcc agggtcaggc tcgcctggtg ggagtggtgg gcgcaggagc ccatcagccg
     3541 gtcgcgcacg tcgacgatgc gccggttgta gtccacccag accgggacga tggcgcgcgc
     3601 cagatccccc ggaaaagcga cgacggcagt attgaagtag gccatcgtgg gcgcgccgag
     3661 cagcgtcgtc aggtaggtgc gcggcagcag cggcaggccg aagtgctcga acaggcgctc
     3721 gaacgcctcg tgcggcaccg acggcacgtc cgcgatcttg gccgccagcg ccccgcgctg
     3781 gaggaacggc agcgggtcgc gcaccaccac catgtcgcag tccagcagca ggatcaggtc
     3841 gctgtccctg gccgtccggg tggcctcctc caggaaccgc agcttgttgg cggtcgggtt
     3901 gcgcgggtcg aaccgctgga cgacgcggac ctcggcgccg tagccctcga gctcccgccg
     3961 cgcctcgggg tcgatcgcct ccacggcgca gacgatgaac cgtgcgcgcg ccaggtcgcc
     4021 gccgaaccag cgggtggact ggacgagccg cagcgtctcg ccgagcatcg ccggggtatt
     4081 ctcggtgacg cagccggcga tcaccgcctc gtcgcggccg cggccgcgag cggcggcctt
     4141 ccagaaggag ttgaggagcc ggaagccgcg ggtgccttcg aggcgggccc agcgcgcttc
     4201 gtgc
//
LOCUS       DASPJI010000725         4591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_216491, whole genome shotgun sequence.
ACCESSION   DASPJI010000725 DASPJI010000000
VERSION     DASPJI010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216491"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VEL74_20320"
     CDS             complement(<1..765)
                     /locus_tag="VEL74_20320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64939.1"
                     /translation="MIPATRSSASASNLVDYWNEARLRVASATSSSVRPAPRIVPWRA
                     ALEDVLKVGYGVQAQPRFLAGAWQEVRWRTTPSAGALYPFEVIATVIGEGSYLWDVEK
                     GHLVPCDAPPLAREELAEAGLVTRPGDRLEALLTFVARPWLSMKKYRLRGYGYCHLDV
                     GHTATNLALYTAALGHDPVLHLRFSRTFLMEHLKLEGLCREPLAVLSFAAADAGGEPN
                     PPVAPESEVRLPMTGLELPGESERRSWESLQGVLSFD"
     gene            complement(762..2018)
                     /locus_tag="VEL74_20325"
     CDS             complement(762..2018)
                     /locus_tag="VEL74_20325"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018814072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HYG64940.1"
                     /translation="MVEAVSSRLAIHGGEPAVTTPGPHFRWPLITDETRQAVLRQLDE
                     AVSLYDRSGVVARLEQRLEAEYALPHALLFNTGTSALLAMFIGAGLAQGDEVLCPDYT
                     FFASNSPLFFTGAIPVLVDCDRTGNMDPARARELVTARTRAILVTHLWGVPCDMDPLV
                     ELCREHGLLLLEDISHAHGATYKGQPVGTFGHAAALSLQAQKTLTGGEGGVLLTRDDE
                     IFYRALALGHYTKRCLQEIPEDHLLHAYGVTGLGLKLRIHPLAAAIAEQQLDTMPAVL
                     AGRRRYAHLMRRELSAVPGLELPPIPEGAEPSWYGLIFHLDRELARRGLSGQVLAALA
                     AEGCSALDHPKVTAPVHRLPLFREPGRVFPGYASPPEREFPMADEFYARAWKLPVWHR
                     EEDGALVGQYLEAIRKVMHHFSRSCP"
     gene            complement(2012..2710)
                     /locus_tag="VEL74_20330"
     CDS             complement(2012..2710)
                     /locus_tag="VEL74_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG64941.1"
                     /translation="MKRVALITGASRGIGLEVGRQLGAAGYTVVLTARDAEKGAGALA
                     LLRQAGVDAHFQTLDVTDPDRARQVADAVASELGRLDALVNNAAIAIDVRATLETVDP
                     GVTRRTLETNLLGALHCCQAVLPVMRRQGYGRIVNVSSGRGSFSRLGAGGPSYRISKT
                     ALNALTVILADELKETNILVNAMTTGWVRTRLGGLKAPRSVAEGAETAVWLAMLPDGG
                     PRGKFFKDREEFPW"
     gene            complement(2707..>4591)
                     /locus_tag="VEL74_20335"
     CDS             complement(2707..>4591)
                     /locus_tag="VEL74_20335"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG64942.1"
                     /translation="DRRLVACFSGHGGQEPAAIDELRELLHERLPSYMVPAVFVPLPE
                     MPLTPSGKVDRRSLAQRAWEIPAADGESRALLTPVEEIVARLWAEVLRLPSVGPDDNF
                     FQLGGHSLSGAQVISRLRQAFQVDLPLRVLFAAPTVAGIAAEIERRRTDEGAAERPAI
                     GAFRQHRGTPPPLSFAQERFWAGRQIEARTMPSTIPMLTQLEGELDVACLQRALQEIV
                     DRHEVLRTSFAESPDGPVQVVHPEVQVRISIVDLARVAPGDRMAEIRHWSTLDGRTHF
                     DYARAPLFRLTLFRGSVREHALLLTVHHSAFDGWSQDVLTTELIHLYTAFRAGHPSPL
                     PPLAAQYQDFARWQRQTLAGEALAGQVAFWREHLRGARPVDLALGPRPAQPTFVAGFE
                     TFLVPQELERRLDRFAAEQYVTLFMTLLAAFEALLFHETGQDDIVVTCLFANRNQLET
                     EKLIGNFYAGLPLRTRFAGSRTFRDLLERVREVTLAAHEHPDILYEPVMEGMSFLENG
                     DRGGLSTFRLLFQLNKRLAPPEPARTPSSGPMSEPSTPDLAFRRLPFDSGKIRLDLSL
                     FFTQTDRLSGRFKYNRDIFEPERVVRLRERFLQILAAAVADPNCPLTELLSRESELME
                     SAR"
BASE COUNT          728 a         1544 c         1683 g          636 t
ORIGIN      
        1 atcgaacgac aggaccccct ggagcgactc ccagctccgg cgctcgctct ccccgggcag
       61 ctcaagaccg gtcatcggca gccggacctc ggattccggc gcgacgggcg gattcggctc
      121 tccgcccgcg tcggcggcgg cgaaggagag caccgcgagc ggctcgcggc acaggccctc
      181 cagcttgagg tgctccatca agaaggtgcg ggagaaccgc aggtggagca ccggatcgtg
      241 gcccaacgcc gcggtataga gcgccaggtt ggtggccgta tgccccacgt cgagatggca
      301 gtagccgtag ccgcgcaggc ggtacttctt catgctcagc cagggacggg cgacgaaggt
      361 gagcagagct tccaatcggt ctccgggacg ggtgaccaga cccgcctcgg ccagctcctc
      421 ccgggcgagc ggcggcgcgt cgcaggggac gaggtgcccc ttctcgacat cccagagata
      481 gctcccctcg ccgatgacgg tggcgatcac ctcgaagggg tagagcgctc ccgccgaagg
      541 cgtggtgcgc cagcggacct cttgccaggc gccagccagg aaacggggct gagcctgcac
      601 tccgtagccg accttgagga cgtcttcgag agcggcgcgc cagggcacga tccgcggcgc
      661 cggcctgacg gacgaagacg tcgccgacgc gacgcgcagg cgcgcctcgt tccagtagtc
      721 gaccagattg ctcgcagacg cgctcgaacg tgtcgccgga atcatgggca gctccgagaa
      781 aagtgatgca tgaccttgcg gatcgcctcc agatactgtc cgaccagcgc cccatcctcc
      841 tcgcggtgcc agaccggcag cttccaggcg cgcgcgtaga actcgtccgc catggggaac
      901 tcccgctccg gcggggatgc atagccgggg aagacgcggc cgggttcgcg gaagagcggc
      961 aagcggtgca ccggggccgt gaccttcggg tggtcgaggg ccgagcagcc ttcggcggcg
     1021 agagcggcga gcacctggcc gctcagaccg cggcgggcaa gctcgcggtc caggtggaag
     1081 atgagcccat accagctcgg ctcggccccc tccgggatgg gcggcagctc gaggccgggg
     1141 acggcgctca gctcgcggcg catcaggtgg gcgtagcggc gccgcccggc cagcacggcc
     1201 ggcatggtgt cgagctgctg ctccgcgatg gccgcagcga gagggtggat gcgcagcttg
     1261 aggccgagcc cggtgacccc ataggcgtgc agcaggtgat cctcggggat ctcctgcagg
     1321 cagcgtttgg tgtagtggcc gagggccagg gcgcggtaga agatctcgtc gtcgcgggtc
     1381 agcaggacac cgccttcgcc gccggtcagg gtcttctggg cctgcaggct gagggcggcg
     1441 gcgtgaccga acgttcccac cggctgcccc ttgtaggtag cgccatgggc gtgggagatg
     1501 tcttccagga gcagcaggcc gtgctcgcgg cagagctcga cgagcgggtc catgtcacac
     1561 gggacacccc acagatgagt gactagtatg gcccgggtgc gcgccgtgac cagctcccgc
     1621 gcccgcgccg gatccatgtt gcccgtacgg tcgcagtcca ccaggaccgg gatcgcgccg
     1681 gtgaagaaga gcgggctgtt cgaggcgaag aacgtgtagt cggggcagag cacctcgtcg
     1741 ccctgcgcca gtccggcgcc gatgaacatc gcgaggaggg ccgaggtgcc ggtgttgaag
     1801 agcagcgcgt gcggcagggc gtactcggct tcgagccgct gctcgaggcg cgccacgacg
     1861 cccgagcggt cgtagagcga gaccgcctcg tcgagctggc gaagcacggc ctggcgcgtc
     1921 tcgtcggtga tcaacggcca gcgaaagtgg ggcccgggcg tggtgaccgc gggctcgccg
     1981 ccgtggatgg cgagccggct cgaaacagcc tctaccatgg gaactcctcc cggtccttga
     2041 agaacttgcc gcgcggaccg ccgtccggca gcatggcgag ccagacggcg gtctccgccc
     2101 cctccgccac cgagcgcgga gctttcaggc cgccgaggcg ggtgcggacc cagccggtgg
     2161 tcatggcgtt gaccaggatg ttggtctcct tgagctcgtc ggccaggatc acggtcagcg
     2221 cgttcagggc ggtcttcgag atgcggtagc tgggcccgcc ggcacccagc cgggagaacg
     2281 acccccgccc gctcgacacg ttgacgatcc ggccatatcc ctgccgccgc atgacgggga
     2341 ggacggcctg gcagcagtgg agcgcgccga ggaggttggt ctcgagcgtc cgccgcgtca
     2401 cgccgggatc caccgtctcc agggtcgccc gcacgtcgat ggcgatggcg gcgttgttga
     2461 ccagcgcatc cagccggccg agctcggagg ccacggcatc ggccacctgc cgggcccgat
     2521 ccgggtccgt cacgtccaga gtctggaagt gggcgtccac ccccgcctgc cgcaggagag
     2581 ccagggcccc ggcccccttt tccgcgtcgc gcgcggtgag cacgaccgtg tagccggcgg
     2641 cgccgagctg gcggccgact tccaggccga tgccgcggct cgcgccggtg atcagggcca
     2701 ctctcttcac cgcgcgctct ccatgagctc actctcccgc gacagcagct ctgtcaatgg
     2761 acagttcgga tcggcgacgg cagcagcgag gatctggagg aagcgttcgc gcagacggac
     2821 gacccgctcc ggctcgaaga tgtcgcggtt gtatttgaag cgcccggaga gccggtccgt
     2881 ctgggtgaag aacaggctca ggtccagacg gatcttgccg ctgtcgaacg gcaggcgccg
     2941 gaacgcgaga tccggggtgg acggttcgga catcgggccg ctggacggcg tccgggcggg
     3001 ctccgggggg gccagccgct tgttgagctg gaacagcagg cggaacgtgg agaggccgcc
     3061 gcggtcgccg ttctcgagga agctcatccc ttccatcacc ggctcgtaga ggatgtccgg
     3121 gtgctcgtgg gccgccagcg tcacctcgcg cacccgctcc aggagatcgc ggaaggtgcg
     3181 ggagccggcg aaccgggtgc gcagggggag cccggcgtag aagttgccga tcagtttctc
     3241 ggtctcgagc tggttgcgat tggcgaaaag gcaggtgacg acgatgtcgt cctgccccgt
     3301 ctcatggaag agcagcgcct cgaaagccgc gaggagcgtc atgaacagcg tcacgtactg
     3361 ctccgcggca aaccggtcga gcctcctttc gagctcctgc ggcaccagga aggtctcgaa
     3421 cccggcgacg aacgtcggtt gggcgggccg cggcccgagg gcgaggtcca ccgggcgggc
     3481 ccctcgcagg tgctcgcgcc agaacgccac ctgtcccgcc agcgcctctc cggcgagggt
     3541 ctgccgctgc cagcgcgcga agtcctggta ctgagcggca agcggcggca gaggagaggg
     3601 atggccggca cggaaggcgg tgtagagatg gatcagctcg gtggtcagaa cgtcctgcga
     3661 ccagccgtcg aaggcgctgt ggtggacggt gagcagcagc gcgtgctcgc gcaccgagcc
     3721 gcggaagagg gtcagccgga acagcggcgc gcgcgcgtag tcgaaatgcg tcctcccgtc
     3781 gagggtgctc cagtgccgga tctccgccat ccggtcgccg ggcgccacgc gcgccaggtc
     3841 gacgatggag atccggacct ggacctcggg gtggacgacc tggaccggcc cgtccggact
     3901 ttcggcgaag ctcgtccgca gcacctcatg acggtcgacg atctcctgca aggcccgttg
     3961 caggcaggcg acgtccagct cgccttcgag ctgcgtcagc atggggatgg tggagggcat
     4021 cgtgcgggcc tcgatctgcc gccccgccca gaagcgctcc tgcgcgaacg acagcggcgg
     4081 cggggtgccc cggtgctgcc ggaaggcgcc gatggccggc ctttccgccg caccttcgtc
     4141 tgtgcgccgg cgctcgatct cggcggcgat tccggcgacg gtcggcgccg cgaagaggac
     4201 gcgcagaggc agatcgacct ggaacgcctg gcgcaggcgc gagatcacct gagcgccgct
     4261 caacgaatgc ccgccgagct ggaagaagtt gtcgtccggg ccgacgctcg gaagccggag
     4321 gacctccgcc cagagccgcg ccacgatctc ctccacgggg gtgagcaggg cgcgcgactc
     4381 gccatccgcg gcggggatct cccaggcgcg ttgcgcgagg gaacggcggt cgaccttgcc
     4441 gctcggcgtc agcggcatct ccggcagagg cacgaacacc gccggcacca tgtacgacgg
     4501 cagacgctcg tgcagcagct ccctgagctc gtcgatggcc gccggctcct ggccaccgtg
     4561 gccagaaaaa caggccacca gccgccggtc g
//
LOCUS       DASPJI010000726         4180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_724800, whole genome shotgun sequence.
ACCESSION   DASPJI010000726 DASPJI010000000
VERSION     DASPJI010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724800"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VEL74_20340"
     CDS             <1..428
                     /locus_tag="VEL74_20340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64943.1"
                     /translation="DSVRIQLPMRQVILQGIKRAPNAKTLVARLGRKETVFEPCYREE
                     ELIELALDADEYRLLRLVDGNRTLYDICIQGPHSPADNGKALYAFQVLQLVRCSSGGA
                     GGSGGPGGGYPPGPEPKAKDENTGAIKIRFKTQGDKFSS"
     gene            453..770
                     /locus_tag="VEL74_20345"
     CDS             453..770
                     /locus_tag="VEL74_20345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HYG64944.1"
                     /translation="MPIYEYQCQSCGKRSELLQRMADPPLAVCPECGGEVKKLMSAPA
                     VQFKGSGWYVTDYAGKKGGEGKSESKASDSTSKEGGSSDKAASAPAPAAGSSGSSGSS
                     GSD"
     gene            954..2498
                     /locus_tag="VEL74_20350"
     CDS             954..2498
                     /locus_tag="VEL74_20350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HYG64945.1"
                     /translation="MKSTVRVLFVLLALVPILSAVPASATTYQMVSDQTLADQAPVIV
                     EARVVAAEPAPVVGRPATDYQVEVERVIKGDLAGSTVMVRIPGGIGTGGIGLKIWGAP
                     RFREGDRTLLFLSPGKDGVYRPLHLMLGAFHVQKVGDRDVAVRELSETVEVGPQGLRP
                     GSDIIRDFDGFSAWLHDRSLGVPRPQDYMLATSAGVTEKGLGQATEKYTLLRWPQDDM
                     PVRWFRFDQGQALDWRVHNGGQPGLGLERTVNAFQVALQAWNDDPGSNIRYNYVGTTD
                     ASGGLVESDDTNTILFDDPYRDDPDNAVEGTFRCGRGGVIAIGGPYFTIATRSFRGEK
                     FHEAVEADIVTNDGTDCFFQDNVSVAEEVFAHELGHTLGLGHSEEDDAVMRATAHDDN
                     RGARLHGDDRAAVAFLYSGGTATRAPAAPKKLVAKAVSGTVIRLTWRDMSNNEDGFHV
                     EVKQGNRFVELGVADPNTKQVEIEGLTAGKNYIFRIRSGNSAGFSAYSNQARATTPRA
                     RRNRQR"
     gene            complement(2520..3764)
                     /locus_tag="VEL74_20355"
     CDS             complement(2520..3764)
                     /locus_tag="VEL74_20355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64946.1"
                     /translation="MTAPDRAVTGLLAGCLVFYLLLMALDGAGIPWSPLTLIPALGLA
                     AFLAHRFTRWASSEAPARFPSDLGWGDAVALAALGVFALFSLTLWNAIPDFIYHWGIK
                     GHRFYLERGIDFEYLARSWNWPIHPDYPNLLPILFAATAILGGGFREPVMMLWSTVWL
                     AAMLLAAREVLRRWAPDRFVRQAALAVAALVLAGFGIGHLMAAAADWMIALALLAAVP
                     ALLAPPGRDGDFQIGCAAAFAAASKTEGLPLAALLVLVQFVRHLAFTRPGLSGLVRSA
                     LRLGLPVAAVALPWLAAVRHHGLFQDYNAGSFDWSRASLIFPALWDSVRGGAWHRLEL
                     GLALLPLLALDRRLRPLAAVASLQLAFYLYVYFSARVDVVFLVESSFPRLVLHLLPAI
                     LVGAAVAFPVKKETAGISAGRS"
     gene            complement(3761..>4180)
                     /locus_tag="VEL74_20360"
     CDS             complement(3761..>4180)
                     /locus_tag="VEL74_20360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64947.1"
                     /translation="KLILGFILAWSLRGFLYEVRAGLEGYDHRHERAAGPALWRFGIP
                     QQERLADCLAAVRKHVPAGSYVAFVSPPGPSQGEFFRWRWAAYLLPEMHVLPLESPET
                     PELAQYLVDYRRQMAHPWLTEMRQLPGCRLYTVRRPA"
BASE COUNT          730 a         1448 c         1458 g          544 t
ORIGIN      
        1 ccgattcggt gcgcatccag ctcccgatgc gccaggtgat cctgcagggg atcaagcgcg
       61 cccccaacgc caagacgctg gtggcacggc tcggccggaa agagacggtc ttcgaaccgt
      121 gttatcgcga ggaggagctg atcgagctcg ccctggacgc cgacgagtac cggctcctgc
      181 ggctggtgga cggcaaccgg acgctctacg atatctgcat ccaggggccg cacagcccgg
      241 ccgacaacgg caaggcgctg tacgcgttcc aggtgctcca gctcgtccgg tgctcgtccg
      301 gcggcgcggg cggctctgga ggccccggag gcggatatcc tcctggcccg gagccgaagg
      361 ccaaggacga gaacaccggc gccatcaaga tccggttcaa gacacagggc gacaagttca
      421 gctcgtaact cgacgagggg tggttttctc gcatgccgat ctatgagtat cagtgtcaga
      481 gttgtgggaa gcggtcggag ctgctgcagc gcatggccga tccgccgctc gccgtctgcc
      541 cggagtgcgg cggcgaggtg aagaaactga tgtccgcacc ggccgtccag ttcaagggga
      601 gcggctggta cgtcaccgac tatgccggca agaagggggg ggaggggaag tccgagagca
      661 aggcctcgga ttccacgtcc aaggaggggg ggagctccga caaagcggcc agcgccccgg
      721 ctcccgccgc cggttcctcc ggctcttcag gttcctccgg ctccgactga gaacgcccgt
      781 ccgggggagg agcggaattt tccccctcct ccgtgggttg ccctcgctgg tgtccgggta
      841 aagactggta agatgtacca tcgaggccgg cttgcgatgt agctaacgcc gcttcaccag
      901 cagctccggt ttctcctggc gagggggaac cgggttcaag ggggtttgtc gccatgaagt
      961 ccacggtccg cgttctcttc gtcctcctcg ccctcgttcc gattctctcg gccgttccgg
     1021 cctccgccac cacctatcag atggtctccg accagaccct ggccgatcag gccccggtca
     1081 tcgtcgaggc gcgcgtggtc gcggccgagc cggcccccgt cgtggggcgt ccagccaccg
     1141 actaccaggt cgaggtcgag cgggtgatca agggtgacct cgccggcagc accgtcatgg
     1201 tccggatccc cggcggcatc ggcaccggcg gcatcggcct caagatctgg ggcgcgccgc
     1261 ggttccggga gggcgaccgg acgctgctct tcctctcccc cggcaaggac ggcgtctacc
     1321 ggccgctcca cctgatgctc ggcgccttcc atgtccaaaa ggtcggtgac cgcgacgtgg
     1381 ccgtccgcga gctctccgag acggtcgagg tcggccccca ggggctgcgg ccgggctccg
     1441 acatcatccg cgacttcgat ggcttctcgg cgtggctgca cgaccgctcg ctcggcgtgc
     1501 cccggccgca ggattacatg ctggccacct cggccggcgt gaccgaaaag ggtctcggcc
     1561 aggcgaccga gaagtacacc ctgctccgct ggccccagga cgacatgccg gtccgctggt
     1621 tccgcttcga tcaggggcag gccctcgact ggcgggtcca caacggcggt cagcccggcc
     1681 tgggactcga gcggaccgtc aacgccttcc aggtggccct ccaggcgtgg aatgacgatc
     1741 cgggcagcaa catccgctac aactacgtgg gcacgacgga cgccagcggc ggtctcgtcg
     1801 agtcggacga taccaacacc atcctgttcg acgaccccta tcgtgacgac cccgacaacg
     1861 cggtcgaggg gacctttcgt tgcggccggg gcggcgtgat cgccatcggc ggcccctact
     1921 tcaccatcgc cacccggagc ttccggggtg agaagttcca cgaggcggtg gaagccgaca
     1981 tcgtcaccaa cgacggcacg gactgcttct tccaggacaa cgtcagcgtc gccgaggagg
     2041 tcttcgccca cgagctcggg cataccctcg gcctcggcca ctcggaggag gacgacgcgg
     2101 tcatgcgggc cacggcccat gacgacaacc gcggcgcccg cctgcacggg gacgaccggg
     2161 cggcggtggc cttcctgtat agcggaggca ccgccacccg cgcgccggcg gctcccaaga
     2221 agctcgtcgc caaggccgtc tcgggcaccg tgatccgcct gacctggcgc gacatgtcca
     2281 acaacgagga cggcttccac gtcgaggtca agcagggcaa ccggttcgtg gagctcggcg
     2341 tcgcggaccc caacaccaag caggtcgaga tcgagggcct caccgccggc aagaactaca
     2401 tcttccggat ccgctcgggc aactccgccg gcttctccgc ctacagcaac caggcgcggg
     2461 ccaccacgcc acgggcacga cgtaaccggc agcgataggt tccggagcca agctcccgtt
     2521 caggaacggc cggcagagat gccggccgtt tcctttttga cggggaaagc gaccgcggct
     2581 cccacgagaa tcgccggcag gagatgcagg accagccgcg ggaagctcga ctccaccaga
     2641 aacacgacgt cgacccgggc gctgaagtag acgtagagat agaaggcgag ctgcagcgag
     2701 gccacggccg ccaggggacg cagcctgcgg tcgagggcca gcagcggcag gagcgccagg
     2761 cccaattcca gccggtgcca ggcccctccc cggaccgagt cccacagggc cgggaagatc
     2821 agggaggccc ggctccagtc gaaggaaccc gcgttgtaat cctgaaaaag gccgtggtgg
     2881 cggaccgccg cgagccaggg gagcgccacc gccgccaccg gaagcccgag ccggagggcg
     2941 ctccgcacga ggccggacag cccggggcgc gtaaaagcca ggtgccggac gaactggacc
     3001 agcacgagca gcgccgcgag cggcaggccc tccgtcttcg aggcggccgc gaacgccgcc
     3061 gcgcagccga tctggaagtc gccatcccgg ccgggcgggg ccagcagggc gggcacggcc
     3121 gcgagcagag ccagagcgat catccagtcc gcggccgccg ccatgaggtg gccgatgccg
     3181 aaccccgcca ggacgagggc ggccacggcg agggccgcct gccggacgaa gcggtcgggg
     3241 gcccaccgcc gcagcacctc ccgcgccgcc agcagcatcg ccgccagcca gacggtcgac
     3301 cagagcatca tcaccggctc ccggaagccg ccgccgagga tcgcggtggc ggcgaacagg
     3361 atcggcagga ggttgggata gtcggggtgg atcggccagt tccagctccg cgccaggtac
     3421 tcgaagtcga tcccccgctc gagatagaag cggtgcccct tgatccccca gtgatagatg
     3481 aagtcgggga tggcgttcca caaagtgagc gagaagaggg cgaacacccc gagggcggcc
     3541 agcgccacgg cgtcccccca gcccaggtcg gaggggaacc gcgccggcgc ctcggaggag
     3601 gcccagcggg tgaaacgatg cgccaggaag gccgccagcc ccagggccgg gatcagggtg
     3661 agcggcgacc aggggatgcc ggcgccgtcc agcgccatca gcagcagata gaagacgagg
     3721 cagccggcga gcaggccggt gacggcgcgg tcgggagcgg tcacgccggc ctccgcaccg
     3781 tgtagagccg gcagccgggg agctgccgca tctcggtcag ccaggggtgc gccatctgcc
     3841 gccgatagtc caccagatac tgcgccagct ccggcgtctc cggcgattcc agggggagga
     3901 cgtgcatctc gggcagcagg tacgcggccc agcgccaacg gaagaactcc ccctggctcg
     3961 gtcccggcgg cgagacgaac gccacatagc tgccggcggg cacgtgcttg cggacggcgg
     4021 cgaggcagtc cgccaagcgc tcctgctggg ggatgccgaa gcgccagagg gcgggcccgg
     4081 cggcccgctc gtgccggtgg tcgtacccct ccaggcccgc gcggacctcg tagaggaagc
     4141 cacggaggct ccaggcgagg atgaacccca ggatcagttt
//
LOCUS       DASPJI010000727         4089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_790673, whole genome shotgun sequence.
ACCESSION   DASPJI010000727 DASPJI010000000
VERSION     DASPJI010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790673"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4089)
                     /locus_tag="VEL74_20365"
     CDS             complement(<1..>4089)
                     /locus_tag="VEL74_20365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG64948.1"
                     /translation="LVARHEILRTLFHRLPGRSWPAQVVLDEEWRPRIELLDLSGREG
                     EVRTAALEGLWEEMLDRPFAPDREPPLTVALVRLAAREHVLVVGAHALCADAAALEIL
                     VRDLRQAYAARLGLGGPGSEEPAQYADVAEGLNELLEAEETDAGRELWREMRAGHAVR
                     ELPFEREPDPGGRTAFRPRVVGAAIGRETLERAEELAGAASCPLSVVLLAAWQVLLHR
                     LTAAEGEPWTLGVDFEGRHYEGFDTALGLFVRTLPLPVAVREEGPFRALLARLDQQVE
                     ALYDWQEYFSWERLPEGERGGFFAAGFGFEEPREAAESAGGVSFSQERTYACVERFRL
                     KLVCRRQQEGLRIELHFDASRFAEEDVRRLLDAFRALLANAVAAPDSRVDELDLIDAA
                     EHRRLAAAHEATARRFEPVPVHHLIREQAGRTPGAVALSTDDDRLTYRELAERARRLA
                     GHLRRLGVRPDVRVGLCVERSVEMVVGILGILESGGVYVPVDPDHPAARQAFVLEDSK
                     AAVLLTRPPLLAKLPPVNGGTRVAFLDEPETWSDTGEEAWPPPDPENAAYVLYTSGST
                     GVPKGVVVRHRALTNHMLWMREAYPLAADDRVLQKTPFGFDASVWELFLPLMMGAELV
                     LAAPGAHREPALLARTLAARRITVLQVVPSLLRLLLEEEAFAGCASLTRVFCGGEALW
                     GDLRDRFYGVLPGAQLVNLYGPTETTVQISSWVAERTVSERPEMAVPIGLPIHNARLY
                     LLDRRLLPVPDGFLGEVWIGGEPPARGYLDRPGLTAASFLPDPFAAEPGGRMYRSGDL
                     ARRRQDGALEFVNRAGQQIKIRGNRFDPGEIEVILRTHPRVAEAVAMVREDQPGDERL
                     VAYVVPRGEAHVAAELRALLAERVPEYMVPTSYVVLPTLPLTPNGKLDRAALPAPSEP
                     SRSEADFAAPRSAVEEVLAQIWADLLGVERVGIHDNFFDLGGHSLLGTQLIYRVREAI
                     GVELSLRGVFESPTVAGIAEQVEELWRVPQSAPIAPVPRNGEIPLSFAQQRLWFIDQL
                     EPGSAVYNTPYFLRLTGELDVPALAAALAGIVERHEVLRTTFAAAEGGAAVQVIGLPF
                     APSLPLTDLGGLSQGSREAEARRLATLEAGRPFDLRSGPVLRTSLLRLANREHVLLVN
                     AHHIATDAWSMEILWREVSVLYEGFRQGRPAVLPALPIQYADFAVWQRGWFQGEVLES
                     QLAYWRRRLGDDPPVVELPADRARPAVQSYRGADLPLTLPAAASTGLHSLSRRAGATP
                     FMTLLALFVALLGRMTGQRDLPVGTPISGRRQPEVGGLVGFFLNTLVIRAELPEGATF
                     RELVAQVRTLALEAYAHQDVPFEKLVDELGPRRSLSHSPLFQV"
BASE COUNT          634 a         1495 c         1476 g          484 t
ORIGIN      
        1 cacctggaag agcggcgagt ggctgaggct gcgccgtggg cccaactcgt cgaccagctt
       61 ctcgaaagga acgtcctggt gggcataagc ctcgagcgcc agcgtccgca cctgcgcgac
      121 gagctcgcgg aaggtggccc cctccggcaa ctcggcccgg atcaccagag tattcaagaa
      181 gaagccgacc agccccccga cctcgggctg gcgccggccc gagatcggcg tcccgaccgg
      241 caggtcgcgc tgccccgtca tccggccgag gagagcgaca aagagggcca gcagggtcat
      301 gaagggggtc gccccggcgc ggcgggagag agagtggagc cccgtggagg cggcggccgg
      361 aagcgtcagg ggcaggtcgg cgccacgata gctctgcacc gccggccggg cccggtccgc
      421 cggcagctcc accacgggcg ggtcatcccc gaggcgccgc cgccagtagg cgagctggga
      481 ttccaggacc tccccctgga accatccgcg ctgccagacc gcgaaatcgg cgtactggat
      541 cggcagggcc ggcaggacgg ccgggcgccc ctgccggaag ccttcataga gaaccgacac
      601 ctcacgccac aggatctcca tggaccacgc gtcggtcgcg atgtggtggg cgttgaccag
      661 caggacgtgc tcacggttgg ccaggcgcag cagggaggtc cggagaaccg gaccagaccg
      721 caggtcgaag gggcgcccgg cctccagcgt cgccagccgg cgcgcctcgg cttcgcggct
      781 cccctgcgag aggcctccca ggtcggtcag cggcaggctc ggagcgaagg ggaggccgat
      841 cacctggacg gcggcgccac cttcagcggc ggcgaaggtg gtccgcaaga cctcgtgccg
      901 ctcgacgatc ccggcgaggg ccgcggcgag cgccggcacg tccagctcgc ccgtcaggcg
      961 caggaagtaa ggggtgttgt agaccgcgct gccgggctcg agctggtcga tgaaccagag
     1021 ccgctgctgg gcaaaggaga gggggatctc gccattccgc ggcaccggcg cgatgggagc
     1081 ggactgcggc acccgccaca gctcctccac ctgctcggcg atcccggcca ccgtggggct
     1141 ctcgaagacg ccgcgcagcg acagctcgac accgatggcc tcgcgcaccc gatagatgag
     1201 ctgcgtgccg aggagcgaat gcccgcccag gtcgaagaag ttgtcatgga tgcccacgcg
     1261 ctcgactccc agcaggtcgg cccagatctg ggccagcacc tcctcgaccg ctgagcgcgg
     1321 cgccgcgaaa tcggcctcgg accgcgaagg ctccgacggc gccggcaggg cggcgcggtc
     1381 cagcttgccg ttcggtgtga gcggcagcgt cggcaggacg acgtaggaag tggggaccat
     1441 gtactccggc acgcgctcgg cgaggagcgc ccgcagctcg gccgctacgt gggcctctcc
     1501 ccgtggcacc acataggcca ccagccgctc gtctccgggc tggtcctccc gcaccatcgc
     1561 caccgcctcc gccacccgcg gatgggtgcg caggatcacc tcgatctcgc cgggatcgaa
     1621 gcggttgccc ctgatcttga tctgctgccc cgcgcggttg acgaactcga gcgcgccgtc
     1681 ctgccgccgc cgggccaggt cgccgctccg gtacatgcgt ccgcccggct ccgcggcgaa
     1741 cggatcgggc aggaagctgg ccgcggtgag gccggggcgg tccagatagc cgcgggccgg
     1801 cggctcgccg ccgatccaga cctcgccgag gaagccgtcc ggcaccggca gcagccgccg
     1861 gtccagcagg tagagacggg cgttgtggat ggggaggccg atggggaccg ccatttccgg
     1921 cctctccgag acggtgcgct ccgccaccca ggaggagatc tgcaccgtcg tctcggtggg
     1981 gccgtagagg ttgaccagct gcgcgccggg caagacgccg tagaaacggt cccgcaggtc
     2041 gccccagagc gcctcgccgc cacagaaaac ccgcgtgagg gaggcacagc cggcgaaggc
     2101 ctcctcctcc agcagcaggc gcagcagcga ggggaccacc tgcaggaccg tgatgcggcg
     2161 ggcagccagg gtgcgggcca gcagcgcggg ctcgcggtgc gcgcccggcg cggcgaggac
     2221 cagctccgct cccatcatca gaggcaggaa gagctcccac accgaggcgt cgaagccgaa
     2281 gggggtcttc tgcaggacgc ggtcgtccgc cgccagcgga taggcctccc gcatccacag
     2341 catgtggttg gtgagagcgc ggtgccggac caccacccct ttgggtactc cggtggagcc
     2401 cgaggtgtac agcacgtagg cggcgttctc cgggtccggt ggaggccatg cctcctcgcc
     2461 ggtgtcggac cacgtctcgg gctcgtcgag gaaggctacc cgcgtccccc cgttcaccgg
     2521 cgggagcttc gccaggaggg gcggccgagt cagcagcacc gccgccttcg agtcttccag
     2581 gacgaacgcc tggcgggcgg ccgggtgatc gggatcgacg ggcacgtaga caccgccgga
     2641 ttcgaggatg cccaggatcc ccaccaccat ctccaccgag cgctcgacgc agagccccac
     2701 ccggacatcg ggcctcactc ccaggcggcg cagatgcccg gcaagccggc gggcccgctc
     2761 ggccagctcc cggtaggtga gccggtcgtc atccgtggac agagcgaccg cgccgggcgt
     2821 gcggccggcc tgctcccgga tcaggtggtg gacgggaacg ggctcgaacc gccgggccgt
     2881 ggcttcgtgg gcggctgcca gccgccggtg ctccgcggcg tcgatcagat cgagctcgtc
     2941 gacacgggaa tcgggagcgg cgacggcgtt ggccaacagg gcccggaagg cgtccagcag
     3001 gcgccggaca tcctcctcgg cgaagcggct ggcgtcgaag tggagctcga tccgcaggcc
     3061 ctcctgctgg cgccggcaca ccagcttcag ccggaagcgc tcgacgcagg cgtacgtccg
     3121 ttcctgagag aacgagacgc cgccggcgct ctcggccgct tcccttggct cctcgaagcc
     3181 gaaaccggcg gcgaaaaagc cgccgcgctc cccttcgggc agccgctccc aggagaagta
     3241 ctcctgccag tcgtacagcg cctccacctg ttggtcgagg cgggccagca gggcgcggaa
     3301 cggcccctcc tcgcgcacgg ccaccggcag cgggagcgtg cggacgaaca gacccagggc
     3361 ggtgtcgaac ccctcgtagt ggcgcccctc gaagtcgacg cccaaggtcc agggctcccc
     3421 ctccgccgcg gtcagccggt gcagcagcac ctgccaggcg gccaggagga cgacggacag
     3481 cgggcaggag gccgcgccgg ccagctcctc ggcccgctcg agcgtctccc ggccgatggc
     3541 ggcgccgacg acgcgcggcc ggaaggccgt ccggcccccg gggtccggct cgcgctcgaa
     3601 cggcagctcg cggacggcgt gaccggctcg catctcccgc cagagctcgc gtccggcgtc
     3661 cgtctcctcc gcctccagca actcgttcag accctcggcc acgtcggcgt actgggcggg
     3721 ctcctcgctc ccggggccac ccagccccag gcgggcggcg taggcctgcc gcaggtcccg
     3781 gacgaggatc tccagcgccg cggcatcggc gcagagcgcg tgcgcgccca ccacgagaac
     3841 gtgctcccgg gcagcgaggc ggacgagggc cacggtcagg ggaggctcgc ggtccggagc
     3901 gaagggccgg tccagcatct cctcccagag gccctccagc gccgccgtcc gaacctcccc
     3961 ctcgcggccg gagaggtcga gcagctcgat acggggccgc cactcctcgt ccagaaccac
     4021 ctgggcgggc cagctcctgc cgggcagccg gtggaagagc gtgcgcagga tctcgtgccg
     4081 cgccaccag
//
LOCUS       DASPJI010000728         8331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_103447, whole genome shotgun sequence.
ACCESSION   DASPJI010000728 DASPJI010000000
VERSION     DASPJI010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103447"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VEL74_20370"
     CDS             <1..720
                     /locus_tag="VEL74_20370"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HYG64949.1"
                     /translation="AWIGKRAASIDRRRATPSAQVQPWPAAGLAADLAPTATAAGSGE
                     TTHLSVIDGAGNLVALTTTLNGLFGCGLWVPEAGLFLNNEMDDFTTAPGRPNYFGLVQ
                     GEANAVAPGKRMLSAMTPTLAWKGDEAIAIGGRGGSRIPTQTAQVLLHLLVDGDPLLT
                     ALGRPRLHHQWLPDQLEVEPDALSPETWAELARRGHKIEVKEGPVSAKVYAVRRLADG
                     RVEAAPDPRSTGTGGIVDPER"
     gene            775..2382
                     /locus_tag="VEL74_20375"
     CDS             775..2382
                     /locus_tag="VEL74_20375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64950.1"
                     /translation="MSRIPGRLSRPMALAMSLLLLALGGAAAQQEPPPRAEQRIEEEI
                     AVRETSVVVELPDTLRDGRLGTNDFLVMVDGKLRRVTRAQPLSGDEPWSLVVYVDRVL
                     AKADTQFPALLALAQRSGELAARGSIEVVVADPAPRTVLAATREPLRIREILSGLAGE
                     ARVARQGPDAERAATAALTSDAPDAQVVLRRQLDRLLTRLADRTTAGPRALLLVADGM
                     DLPPEQVERLARVQMGAMTSAPAPSGAAALFVEASRLLAAYGWVTFAMPFRDLGDGPG
                     EANRGTNETTRVRQSADYGDLSSTVPPMILQARPGGSPSALQLPRVIDLFVLPNLAPL
                     RALAQPTAGTVVAFPEQLDFVLRGLGRRWEVSYESPESVNGRLSSFEVMVPASQVRPR
                     TQRWIRSSTPEEITEARLRLLVGGGTPANDRAGALPATISAAAREDGLALHVEVAPFP
                     SQGSPPAGPVRISHALVRQGGEIQIRHHLAPGIARPDQGWSRDLTIPLPKGTLGVAVI
                     VEDLARARWSATMMAPGAAAGAAGPTR"
     gene            complement(2392..3921)
                     /locus_tag="VEL74_20380"
     CDS             complement(2392..3921)
                     /locus_tag="VEL74_20380"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HYG64951.1"
                     /translation="MRSALDVSSTWASSSTVPAGFTVTVTPSSFTLAPGESRTVQITV
                     EAPPGSYGWREGVVVLTPSGGVAPPARLPVAARWVPEYQLTVDVAGGGSGRVTSSPPG
                     IDCGTDCTELYPEETLVTLTPVPSPGSVFAGWSDGWSCGTGPGCTFEMSYPRWVTAYF
                     TVPAPDRLLANGTPLRDSLHGYVENGTWSYYYIDVPAGTAELVVDVLDLTGEVSLFVR
                     QGAKPTGSDYNCYDAWWGGLSRRCVVSQPAAGRWWIGLNNEDTGNIAFSVRAAWGTAN
                     GSDRLLTNGLVAGDFLTASSSGEAWKYYFFDVESGATNLTVELADLSGDVDLLVRRGA
                     KPDRSTHDCDSSQGSTLTERCSFANPQPGRWWVAVNNFGTGTLHFKVRAGWTTPPTSF
                     YTVQPCRVLDTRSSSPLVPGPSRIFQVTGACGIPATARAVVANVTAVLPTGQGHVTLH
                     PGNQPPPLASSVNFRAGQVRANNGILNLPADGGGALGASTALLGGGHVHLLVDVTGYF
                     Q"
     gene            complement(3978..5867)
                     /locus_tag="VEL74_20385"
     CDS             complement(3978..5867)
                     /locus_tag="VEL74_20385"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HYG64952.1"
                     /translation="MEIAPRRLLVSVCLCVLVLAVPVSAEEPAGPGVYVVRLEDRPVA
                     GYRGGVPSFAATRPEAGPRARGRRDRLDAGSAASRSYAGYLRQRQDETLVRIAKVLGR
                     QAEPLARYAVATHGFALELSPGEAKRIARVPGVAQVERSREMVPQSDAGPAWIGADTI
                     WDGSGTGGLPGTRGEGMVVGVIDTGLNAGHPSFADVGGDGYDHTNPRGAGQYVGRCDP
                     ADPAYDPAIPCNDKLIGLWAYGVGSAPAPDETGHGTHVAAIAVGNHLSATVSGPGIDL
                     VRDVSGVAPHANLIAYDACPTSHSCSSYLFLEAVDQAVADGVDVVALGFGAYLTSDPW
                     EDALAAALLNAREAGVFVAVPAGNDGFTTYLSSVAELPWVMALTATTHHRRLVASLKT
                     MSGGDSPPPADIQGWGLSGPYGPAPIVPAWDYGDGECTVPFPAGTWSGEIVVCAYYPW
                     NGAAQVSANVLAGGAGGVLLADYYSFFEDYPPPIEGPDLPWIYIRDNIVYALDEWLGS
                     GAGHTAVLSGTLVETHPSLGDIFYAGSSRGPSLTVPGVVKPDLAAPGVDILAADHDSQ
                     GFVFQTGTSMGPWSGRTGRPPRRWRSEPGGSTPAPPPVPACSSTSPAPSSPRPTPRWA
                     GIRRP"
     gene            6090..6542
                     /locus_tag="VEL74_20390"
     CDS             6090..6542
                     /locus_tag="VEL74_20390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016470950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HYG64953.1"
                     /translation="MAKGSPGKGCVFCSIVRGETAAATVHEDELTLVFLDHRPLLPGH
                     CLVVPKLHAPTLAELPEGLVAPLFGTVQLLARAVEEAMEADGSFTAINIKISQSVPHL
                     HVHVVPRWKGDGLFSPKLVWKRTPYESDAARAAVQAKIRGALERLGGP"
     gene            6602..>8331
                     /locus_tag="VEL74_20395"
     CDS             6602..>8331
                     /locus_tag="VEL74_20395"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HYG64954.1"
                     /translation="MPATAIDFAIRRREMVDQQIARRGVRDPRVLEALATVPREAFVP
                     EHLAEFAYDDTPLPIGEEQTISQPFIVALMAEALELQAGDRVLEIGAGSGYAAAVLSR
                     LAGEVYAIERHASLAKQASRRLEELGYGNVRVHHGDGTLGWPEAAPYDAIVVAAGGPD
                     VPPALVDQLAPGGRLVIPVGSDPRLQKLIRLRRGADGVDRREDLGDVRFVPLVGVQGW
                     TESGRQAPVLSGLPEPPAVIAPAPAPPRELPALIREAAEPFGSIDSIGDASLDALLQR
                     IGDARLVLLGEATHGTSEFYRMRARITRELVTRKGFNVVAVEADWPDAVQVDRWVRGR
                     SAPGSHDRERRERAFARFPTWMWRNHDVRDFIHWLRDHNAGVADPARRVGFYGLDLYS
                     LYSSIHAVTRYLDQVDPEAARIARLRYGCLTPWEQDPALYGQAALMGRYDSCEQGVVE
                     TLVKLLEKRVEYESLDGEHFLEAVQNARVAANAERYYRIMYYGSRDSWNLRDHHMFDT
                     LESVLAFRGPESKAVVWAHNSHLGDARATQMGDVGELNVGQLMRQRHGDEVFSIGFST
                     HTGTVTAAHD"
BASE COUNT         1131 a         3094 c         3013 g         1093 t
ORIGIN      
        1 gcctggatcg ggaagcgcgc cgcgagcatc gaccgccggc gggccacgcc ctcggcccag
       61 gtccagccct ggcccgcggc cggcctcgcg gccgacctcg cgccgacggc caccgcggcg
      121 gggagcggcg agaccactca cctctcggtc atcgacggcg ccggcaacct ggtggcgctc
      181 accacgacgc tcaacggcct gttcggctgc ggcctctggg tgcccgaggc cggcctcttc
      241 ctcaacaacg agatggacga cttcaccacg gcgccggggc ggccgaacta cttcgggctc
      301 gtccaggggg aggccaacgc cgtcgccccc ggcaagcgga tgctctccgc catgaccccc
      361 accctcgcct ggaaggggga cgaggccatc gccatcggcg gccgtggcgg ctcgcgcatc
      421 cccacccaga cggcgcaggt gctgctgcat ctgctggtgg acggcgatcc gctcctgacc
      481 gccctcggcc gtccgcgcct ccaccaccag tggctccccg accagttgga ggtggagccc
      541 gacgccctct cccccgagac ctgggcggag ctggcgcgcc gcggccacaa gatcgaggtg
      601 aaggaggggc cggtctccgc caaggtctac gccgtccgcc gcctcgccga cggccgggtg
      661 gaggcggccc ccgacccgcg gagcacgggt acgggcggga tcgtggatcc ggaacggtag
      721 acgcccccgg cacgggtttt gcccctccct ctcgcagact ggaggaggca acgcatgtcc
      781 cggatcccag gcaggctctc ccgaccgatg gcgctggcca tgtctctgct cctcctggcg
      841 ctggggggag cggcggccca gcaggagcct ccgccgcgcg ccgagcagcg catcgaggag
      901 gagatcgcgg tgcgcgagac cagcgtcgtg gtcgagctgc ccgacacgtt gcgcgacggc
      961 cggctgggta cgaacgactt cctggtgatg gtggacggca agctccggcg ggtgacccgg
     1021 gcgcagccgc tctccggcga cgagccctgg agcctggtcg tctatgtgga cagggtgttg
     1081 gcgaaagcgg acacccagtt cccggccctc ctcgccctcg cccagcgctc cggcgagctg
     1141 gccgcccgcg gcagcatcga ggtggtggtc gccgatccgg cgccgcgcac cgtcctcgcc
     1201 gccacccggg agccgctgcg catccgggag atcctctccg gcctcgccgg cgaggcccgc
     1261 gtcgcgcggc aggggccgga cgcggagcgc gcggccacgg ccgcgttgac ctcagacgcc
     1321 ccggacgccc aggtcgtcct ccggcgccag ctcgaccgcc tgctgacgcg cctggccgac
     1381 cgcaccaccg ccggcccacg ggccctgctc ctggtcgccg acggcatgga cctgccgccg
     1441 gagcaggtcg agcggctggc gcgcgtgcag atgggggcga tgaccagcgc gcccgcccct
     1501 tccggggcgg ccgccctctt cgtcgaggcg tcgcgcctcc tcgccgcgta cggctgggtg
     1561 accttcgcca tgccgttccg cgacctcggc gacgggccgg gcgaggccaa ccgcggcacc
     1621 aacgagacca cacgggtgcg gcagagcgcc gactacggcg acctgagctc caccgtgccg
     1681 ccgatgatcc tccaggcgcg gccgggagga tccccctcgg ccctgcagct gccgcgggtg
     1741 atcgacctct tcgtcctgcc gaacctggcg ccgttgcggg cgctggccca gccgaccgcg
     1801 ggcacggtgg tcgccttccc cgagcagctc gacttcgtcc tgcgcggcct cggccggcgc
     1861 tgggaggtct cgtacgagtc ccccgagtcg gtgaacggcc ggctctcctc gttcgaggtg
     1921 atggtgcccg cgtcccaggt ccgcccgcgc acccagcgct ggatccgctc ctcgacgccg
     1981 gaggagatca ccgaggcgcg cctccgcctc ctggtgggag ggggcacccc ggcgaacgac
     2041 cgtgccggcg ccctgcccgc caccatctcc gccgcggcca gggaggacgg cctggcgttg
     2101 cacgtcgagg tggcgccgtt cccctcccag ggctcgccgc cggcgggacc ggtccggatc
     2161 tcccatgccc tggtccgcca ggggggagag atccagatcc ggcatcatct ggcccccggg
     2221 atcgcccggc cggaccaggg gtggagccgc gacctgacca tccccctccc caaggggacc
     2281 ctcggggtgg cggtgatcgt cgaagacctg gcccgggcgc gctggagcgc caccatgatg
     2341 gcgccggggg cggccgccgg ggcggccggc cccacccgct gagaggtccc cctactggaa
     2401 atagccggtc acgtccacca gcaggtggac gtgaccgccg ccgagcaggg cggtggaggc
     2461 ccccagcgcg ccgccgccgt ccgccggcag gttcaggatg ccgttgttgg cgcgcacctg
     2521 gccggcccgg aagttgaccg agctggccag cggcggcggc tggttgccgg gatggagcgt
     2581 cacgtgcccc tggccggtgg gcagcaccgc cgtcacgttg gccaccacgg cccgcgccgt
     2641 cgccgggatg ccgcacgccc cggtgacctg gaagatccgc gacgggccgg ggacgagcgg
     2701 cgaagagctg cgggtgtcca gcacccggca gggctggacg gtgtaaaagc tggtgggcgg
     2761 cgtggtccag cccgcccgca ccttgaagtg cagggtgccg gtgccgaagt tgttgaccgc
     2821 cacccaccag cggcccggct gcgggttcgc gaagctgcag cgctcggtca gcgtcgagcc
     2881 ctgggacgag tcgcagtcgt gggtggagcg gtccggcttg gcgccgcggc gcaccaggag
     2941 gtccacgtcg cccgacaggt cggccagctc caccgtcaga ttcgtggcgc cgctctcgac
     3001 gtcgaagaag tagtacttcc aggcctcgcc cgaggaggag gcggtcagga agtcgcccgc
     3061 caccaggccg ttggtcagca ggcggtcgct gccgttggcc gtcccccagg cggcccgcac
     3121 cgagaaggcg atgttccccg tgtcctcgtt gttcaggccg atccaccagc ggccggcggc
     3181 gggttgggag acgacgcagc ggcggctgag gcccccccac caggcgtcgt agcagttgta
     3241 atccgacccg gtgggcttgg cgccctgacg gacgaagagc gagacctcgc cggtgaggtc
     3301 gaggacgtcc accaccagct cggcggtccc ggccggcacg tcgatgtagt agtagctcca
     3361 ggtgccgttc tccacgtagc cgtgcagcga gtcccggaga ggggtgccgt tggccaacaa
     3421 ccggtcgggg gcgggcaccg tgaagtaggc cgtcacccac cgcgggtagc tcatctcgaa
     3481 cgtacagccg gggcctgtgc cgcaggacca gccgtcggac cagccggcga agaccgagcc
     3541 ggggctgggg accggggtga gtgtcaccag ggtctcctcg gggtagagct ccgtgcagtc
     3601 cgtgccgcag tcgatcccgg gcggagagct ggtgacccgg ccactgcccc cgccggccac
     3661 gtcgacggtg agctggtact ccggcaccca ccgggccgcc acgggcagcc gcgccggcgg
     3721 cgccaccccg cccgacggcg tgaggacgac gaccccctcc cgccagccat agctgcccgg
     3781 cggggcttcg accgttatct ggacggtccg gctctccccc ggggcgaggg tgaaggagga
     3841 gggcgtgacg gtgaccgtga agccggccgg aaccgtggac gacgacgccc aggtagacga
     3901 gacgtccagc gcgctccgca ccgtccgcgt ccaggtgcag gtcttgacgc agaagccgtc
     3961 ccccaggctg ggcaggttca gggcctccgg atgcccgccc agcgaggggt cggcctgggc
     4021 gagctcggcg ccggtgacgt cgaggagcag gccggcacgg gcggcggcgc cggcgtcgag
     4081 ccgcccggct ccgacctcca gcgcctgggc gggcgtcccg tcctcccaga ccacggtccc
     4141 atggaggtgc cggtctggaa gacgaaaccc tgggagtcgt ggtcggcggc caggatgtcg
     4201 acgccgggag cggccaggtc cggcttgacc acgccgggta cggtgaggct gggcccgcgc
     4261 gagctcccgg catagaagat gtcgcccagg gagggatggg tctccacgag ggtgccggag
     4321 aggacggcgg tgtggcccgc gccggagccc aaccactcat cgagcgcgta gacgatgttg
     4381 tcgcggatat agatccaggg gaggtccggg ccttcgatcg gtggcgggta gtcctcgaaa
     4441 aagctgtagt agtcagccag caacacaccc ccggcgccgc cggccaggac gttggcgctc
     4501 acctgcgccg ccccgttcca cgggtagtag gcgcaaacta cgatctcccc gctccaggtg
     4561 cccgccggga acgggaccgt gcactccccg tcgccgtagt cccaggccgg gacgatcggc
     4621 gccgggccgt aggggccgct caacccccac ccctggatgt cagcgggcgg cggcgaatcg
     4681 ccgccactca tcgtcttcag ggaggcgacg agccgccggt ggtgggtggt ggcggtgagc
     4741 gccatgaccc aggggagctc ggcgaccgag gacaggtacg tggtgaaccc gtcattgccg
     4801 gccggcacgg ccacgaagac ccccgcctcg cgggcgttca gcagcgccgc cgccagagcg
     4861 tcttcccagg ggtccgacgt cagataggcg ccgaagccga gggccaccac gtccacgccg
     4921 tccgccaccg cctggtccac ggcctccagg aacaggtagg acgagcagga gtggctggtc
     4981 gggcaggcat cgtaggcgat gaggttggcg tgcggcgcca cgccggagac gtcgcggacg
     5041 aggtcgattc ccgggccgct cacggtggcg gagaggtggt tgcccaccgc gatggccgcc
     5101 acgtgcgtgc catggccggt ctcgtccggc gccggagcac tcccgacgcc gtaggcccac
     5161 agcccgatga gcttgtcgtt gcaggggatg gccggatcgt aggcggggtc ggcgggatcg
     5221 caccggccga cgtactggcc cgcaccccgc gggttggtat ggtcgtagcc gtccccgccc
     5281 acgtccgcga aggaggggtg gccggcgttc aggccggtat cgatcactcc gaccaccatg
     5341 ccctcccccc gggtgcccgg caggccgccg gtgccgctgc cgtcccagat cgtgtccgcc
     5401 ccgatccagg ccgggccggc gtcggactgc ggcaccatct cgcggctgcg ctcgacctgg
     5461 gcgacgccgg ggacacgggc gatccgcttc gcctccccgg gcgacagctc cagggcgaag
     5521 ccgtgggtcg ccacggcgta gcgggccagc ggctcggcct ggcggccgag caccttggcg
     5581 atccggacga gcgtctcgtc ctgccgctgc cgcaggtagc cggcatagct ccggctggcg
     5641 gcggacccgg cgtcgagccg gtccctgcgg ccgcgggcgc ggggaccggc ctcggggcgg
     5701 gtggcggcga agctcggaac tccgccccgg tacccggcca cgggccggtc ctcgagccgc
     5761 acgacataga cgccgggccc tgccggctct tcagccgaca ccggcaccgc caggaccagg
     5821 acacacagac acacggacac caacagccgt ctcggggcga tttccacggg gaggccctcc
     5881 ttcggattgg agtgattctc actggaattc tatttcgcca cctccgggct caacaaacgc
     5941 cccggaaccg tgtctaatag agccgaggcc ccgcggctcg cgagaccgcg gggcccccct
     6001 gtagtccggc ccagggcgct cccccgcacc ctgggtcctt ttttgttctc gccgcggcct
     6061 cccgccgggg aggcgataga ctgtcgcgca tggccaaggg gagcccgggc aagggctgcg
     6121 tcttctgctc gatcgtccgc ggcgagaccg ccgccgccac cgtccacgag gacgagctca
     6181 ccctggtctt cctcgaccac cgcccgctcc tgcccgggca ctgcctggtg gtgccgaagc
     6241 tccacgcgcc gacgctggcc gagctgcccg aggggctggt ggcccccctc ttcggtaccg
     6301 tccagctcct cgcccgcgcc gtcgaggagg cgatggaagc ggacggctcc ttcacggcga
     6361 tcaacatcaa gatcagccag agcgtgccgc acctgcacgt ccacgtggtc ccccgctgga
     6421 agggggacgg cctcttctcc cccaagctcg tctggaagcg caccccctac gagagcgacg
     6481 ccgcccgggc cgccgtccag gcgaagatcc gcggcgcgct ggagcggctc ggcggtccct
     6541 aggcgggctt ctcggccggg aagccttttt gcaagcttcc cccgcctggg aggactcgcc
     6601 aatgcccgca accgccatcg acttcgcgat ccggcgccgg gagatggtcg atcagcagat
     6661 cgcccggcgc ggcgtccgtg atccccgcgt gctggaagcc ctggcgaccg tgccccggga
     6721 ggccttcgtg ccggagcacc tcgccgaatt cgcctacgac gacacgccgc tccccatcgg
     6781 cgaggagcag accatctccc agccgttcat cgtcgccctg atggccgagg ccctggagct
     6841 gcaggcgggt gaccgcgtgc tggagatcgg cgccggctcg gggtacgccg cggcggtgct
     6901 ctcccggctg gccggcgagg tctacgccat agagcgccac gcctcgctgg cgaagcaggc
     6961 gagccggcgg ctggaggagc tgggctacgg gaacgtccgc gtccaccatg gcgacggcac
     7021 cctcggctgg cccgaggccg cgccctacga cgccatcgtc gtcgcggccg gtggccccga
     7081 cgtgccgccg gccctcgtgg accagctcgc cccgggcggg cggctggtga tcccggtggg
     7141 ctccgacccg cgcctccaga agctgatccg gctgcggcgc ggtgccgacg gcgtggaccg
     7201 gcgcgaggat ctgggggacg tccgcttcgt ccccctggtc ggggtgcagg gctggaccga
     7261 gagcggccgg caggcgccgg tgctctccgg tctccccgag ccgcccgccg tcatcgcccc
     7321 ggcccccgcg ccgccgcgcg agctgccggc gctgatccgc gaggccgccg agcccttcgg
     7381 ctccatcgat tccatcgggg acgcgagcct cgacgcgctg ctgcagcgca tcggcgacgc
     7441 ccgcctcgtc ctcctggggg aggccacgca cggcacctcc gagttctacc gcatgcgcgc
     7501 ccgcatcacc cgcgagctcg tcacccgcaa ggggttcaac gtggtggcgg tggaggccga
     7561 ttggcccgac gccgtccagg tggaccgctg ggtgcggggc cgctccgcgc ccggatccca
     7621 cgatcgggaa cggcgggagc gggccttcgc gcgcttcccc acctggatgt ggcgcaacca
     7681 tgacgtgcgg gacttcatcc actggctgcg ggaccacaac gccggcgtgg cggacccggc
     7741 ccgcagggtg ggcttctacg gcctggacct ctacagcctc tactcgtcca tccacgccgt
     7801 cacccgctac ctcgaccagg tggaccccga ggcggcgcgg atcgcgcgcc tccgctacgg
     7861 ctgcctgact ccctgggagc aggacccggc gctctacggc caggcggccc tgatggggcg
     7921 ctacgactcc tgcgagcagg gggtggtgga gacgctggtg aagctgctcg agaagcgggt
     7981 cgagtacgag agcctggacg gcgagcactt cctggaggcg gtgcagaacg cccgggtggc
     8041 ggccaacgcc gagcgctact accggatcat gtactacggc tcgcgggact cctggaacct
     8101 gcgggaccac cacatgttcg acaccctgga gtcggtgctc gccttccgcg gcccggagtc
     8161 gaaggcggtg gtctgggcgc acaactccca cctcggcgac gcgcgcgcga cgcagatggg
     8221 ggacgttggg gagctcaacg tcggtcagct catgcgccag cgccacggcg acgaggtctt
     8281 ctcgatcggc ttctcgacgc acaccgggac cgtcaccgcc gcgcacgact g
//
LOCUS       DASPJI010000729        17985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_103448, whole genome shotgun sequence.
ACCESSION   DASPJI010000729 DASPJI010000000
VERSION     DASPJI010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17985)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17985
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103448"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(510..2561)
                     /locus_tag="VEL74_20400"
     CDS             complement(510..2561)
                     /locus_tag="VEL74_20400"
                     /inference="COORDINATES: protein motif:HMM:NF022063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z1 domain-containing protein"
                     /protein_id="HYG64955.1"
                     /translation="MREHWVGIRELNQENADRVIREAHQVLSQCTPPSESTPVRTGLV
                     CGYVQSGKTASMTAVSALARDNRYRVIVLIAGTTENLVTQNRDRLETHLRAAATDWKW
                     LMLTNPRPETERDDLRALAQEWRSEHYEDEDRRTLFISVMKNYSHLDKLSELLEKAGL
                     GNFPAIIFDDEADQASLNTRPNDPIASSTYRHIEELRRALPRHTYLQYTATPQAPLLI
                     TRIDSLSADFAELVSPGESYTGGRDFFARDSLFVELIPQSEIYRDGQLPLEPPQSLLR
                     AMQVFFVGVAAGRLEGGQKHRSMLIHPSKATATHTSYYTWANRLKHDWHEVLLEETDP
                     DREELLREFKRAYEDLERTCQRLPPFEQIQAKLPVAINQTTITPVNSVDGREVRWQNG
                     YSHVLVGGEKLGRGYTVKGLTVTYMPRSPGGWTADTIQQRARFFGYHRDYLGYCRVYL
                     HPDVRRIYEAYVTHEQDMRKRLAEHRGHPLQEWRRLFYMDSRLAPTRRNVLLNPWMRA
                     TLDDGWFAPKAPHLSPEDGVHNNRFLGQLKGLDFRKHETYPRHHYAFVALREFFERVL
                     VPLSYLYEDDALGLCVANCNLKTLLERDENALCKVYLMDGGRVRQRSLRNGLIPQLFQ
                     GRSSAGSVSYPGDRAFSDPNLPTLQIHQLSLSEGGRVIDNIQALAIKLGQHEDVLLHD
                     D"
     gene            complement(2614..4164)
                     /locus_tag="VEL74_20405"
     CDS             complement(2614..4164)
                     /locus_tag="VEL74_20405"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG64956.1"
                     /translation="MVAIRPEVAMYAAFARLNYKPWYALAEFVDNSLQSALANMEALQ
                     RVSGGTYRLSVQIDVSEDYIEIRDTAAGIHQRDYARAFRPASPPPDRSGLSEFGLGMK
                     AASSWFARKWSVRTTALGEAVERTITFDIPEIVTRGREELEPIERKAELTDHYTTLLL
                     SDLNVRPKHRTIWKIKEHLRSIYRLFLRDGLIELRVNDDDLKYENPAILCTPYHAAPT
                     SSPVTWRKEINLDLGDGHRVSGWAAILARASVANAGFSIFRRRRLIQGSHGEGYRPEE
                     IFGKPNKFIYQRLVGEFEVDGFSVSHTKDGIQWDEWEEDILAWLKSVLDAQPLPLLDQ
                     AANYRARATTDRDVVAEAALDTTQVIAQHLPPIVDAQIHADPDESPLASDLDAVSEPS
                     SRSVQVELALEHAQRRWIVTLEVISELTRESWYDIAESQPGADTWRIHIRVNLAHPFM
                     VRFISLDGDEIVPFLRLAAGLAIAEVTAREVGVRQAGTLRRNLNQILRSALSGPIQKG
                     EKPNGKRE"
     gene            complement(4166..5410)
                     /locus_tag="VEL74_20410"
     CDS             complement(4166..5410)
                     /locus_tag="VEL74_20410"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HYG64957.1"
                     /translation="MRKLISLYTGAGGLDLGFEAAGFETAVAVELDAEAVATLRHNRS
                     WPVIDRDIHTVSSKELLDKAGLKSGEPDALIGGPPCQPFSKSGYWVSGDTLRLDDHRA
                     GTLAAYLRVLRDTLPRTFLLENVPGLVYQNKSEGLHLIEQAIEQINQACGTSYTIAVK
                     KINGADFGVPQLRERVFVIGAREGNQFRFPTPTHAPDDEVSPLAPRRKPYTTAWDAIG
                     DLDEKVWPAELNPAGKWAGLLRSIPEGQNYLWHTSEGGGEPLFGWRTRYWSFLLKLAK
                     RRPSWTIQAAPGPATGPFHWRNRLLSIEELARLQTFPRGYYFIGNRRSAHRQIGNAVP
                     CALGELLGLEMRRQFLSDPIPERRLTLIPTRRRDCPPAQRHQSVPSEYLHLRGSYPPH
                     PGTGLGPRRTWLDVATPFSQET"
     gene            complement(6052..6555)
                     /locus_tag="VEL74_20415"
     CDS             complement(6052..6555)
                     /locus_tag="VEL74_20415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64958.1"
                     /translation="MQSNDDGSTRRPSNIFLHEFLQQLRQRDEPSPSVSAEASKAGPW
                     RVEEIPATFYGVYATGQDASRGDRAIAIFGDWYHALLISALIPATGRDPLYRLHKEPT
                     PDGYAVETGGEVIGHLAVFDDKLIDALHVVECLLRSPEALASLFEAAGPVILERAGAI
                     LSGRVRE"
     gene            7107..8564
                     /locus_tag="VEL74_20420"
     CDS             7107..8564
                     /locus_tag="VEL74_20420"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HYG64959.1"
                     /translation="MARPAPRVSHAGLRDFLEIPYDQLEEMNLEAKEERLARRPADVV
                     REQRMRYLNDEKRIKAVTVCFTDLEGRLHMLDYDKKFLLKSADNLTFDGSSIRGFSRQ
                     QESDLRLAVDWPAFYWLPSDIFGPGKVLAFGEVHERDGSLYGADMRAMLKGYTDKLYS
                     RDGMVAHAANEIEGFLFRGRDAERCFQETGRFEFISTGGYYHSLPGDSLRQFIDTAAE
                     VQRAMGFANEKDHPEVAPSQFEMNYSYTEASVAADQVQLYKLLSRQVAARLGMTASFL
                     PKPVTGVNGSGMHTNLSVTRQSTNLFYDKDGKDGLSAMGWDFINRILASATDICLVLN
                     SSVNSYRRLDPHFEAPNQIKASAIDRGSMVRIPLGNERSARVEVRSIAPDSNPYLAIY
                     TLLRTGLEGPQSEEDGDKRPRTRFLPDNIYDAIRLFKGSRFMQEVMGEDVHAKYAEIK
                     LAQAERCPKALGTLIKASEIQFHHEVTNQYLWSQF"
     gene            complement(8692..9129)
                     /locus_tag="VEL74_20425"
     CDS             complement(8692..9129)
                     /locus_tag="VEL74_20425"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG64960.1"
                     /translation="MILFVDSSVLVKLYIAEPGSERMREAAAQGSSVAASRLAFAEIH
                     ATFARRSREGLLLASELEQLRRRFAEDWEGITQMPVGAEVLALIPGLCDRHPLRGADA
                     VHLASALLLDQEGLEITFACSDRLLLGAADSEGLATFNTVQED"
     gene            complement(9126..9398)
                     /locus_tag="VEL74_20430"
     CDS             complement(9126..9398)
                     /locus_tag="VEL74_20430"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HYG64961.1"
                     /translation="MSTRVLKDQLSSYLQRAEAGEQIVVLRDGQPVAALVPLSQLREV
                     DEEARLAELAARGLVALPNRTRSTFLGPSVPSRGKLASEMIIEDRR"
     gene            complement(9559..10749)
                     /locus_tag="VEL74_20435"
     CDS             complement(9559..10749)
                     /locus_tag="VEL74_20435"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HYG64962.1"
                     /translation="MLAERIPEIQDALAEAGLDGWFFMCFQFNDPISLDLLGLVGEGK
                     LVSRRCYYLIPRQGEPRKLVHGLEESMLDHLPGSKAEYTTWQENREQLAKLVSGVERI
                     AAHYSPRNEIPTISRLDAGTAELLRELGVDLVSAADLAQRFAATWSDEQLAGHRRANV
                     HLHSIVKDAFTRVGDILRRGDEVDEYMVQQWIMESFDRAGLITDAAPIIGVNAHSADP
                     HYQPGPDHSSPIRRGDFLLIDLWAKEKAAGSVYADITWCGVCSVTPQERHAEIFRIVA
                     GARDAAWELVRSQYPLRPVRGFEVDDAARAFIRQAGYGERFIHRTGHSIGTTDHGQGA
                     NMDNLETHDTRQLLPMTGFSIEPGIYLTGDFGVRSEINVALTPEGPEITGGEPQRELL
                     RLLA"
     gene            10890..11816
                     /locus_tag="VEL74_20440"
     CDS             10890..11816
                     /locus_tag="VEL74_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HYG64963.1"
                     /translation="MRAFYCDEFVLPLPEGHRFPMEKYRLLRQGVLERGLVAPEDLRV
                     PVAATDEEILRVHEPVYLERVGAGTLERAEVRRIGFPWSPAMVERSRRSAGGTLAACR
                     EALENGSAVNLAGGTHHAFRDRGEGYCVFNDAAIAARAMQAEGRVERVVVLDCDVHQG
                     NGTAAIFQDDPTVFTFSIHGRHNFPFHKERSDLDVELEDGTGDEEYLANLSQGLERVF
                     AAGAAEAGLAIYVSGADPHQGDRLGRLALSRAGLLARDRLVFEACRRCGLPVAVVMAG
                     GYNREVSETVEIHLQTVAEARRHFGMWTRVWD"
     gene            11935..13188
                     /locus_tag="VEL74_20445"
     CDS             11935..13188
                     /locus_tag="VEL74_20445"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG64964.1"
                     /translation="MIRNYRTLVVALLLSLLAAGSVWAIGEGRFTGTVMDQEGKPVAG
                     AKITVTSPGSSYKQEKTTNDKGKFTIMVVDAMRKYEIRIEKEGYSPIQGPLAINPSAP
                     TDQDFTLAVAGPSAEELAEIKGKNDAVAAFNEGVALVKAKDFPGAAGKFEQALTLDPT
                     ITQGHQVLADVYFEMKKYPEAIASADKYLATSPASTNGMYVLKYDSYLAMGDKAKASA
                     ALQEMLQKEPGKDAAVRIFNLGAEAQRKDDDANAEKYLRQAIELDPTLERAYASLGTY
                     YLRQKKYDASLDMADRLLKQNANSAEALSLRYESLKALKRTDEAKQAMAAMQASAGNQ
                     TAEEAFRQGVALYNANNVAEAEKAFERALAKDGNYAKAHYLLGLVEASKGNMAKAREY
                     VQTFLKLAPNDPQAAEAKAVLAELK"
     gene            complement(13587..15359)
                     /locus_tag="VEL74_20450"
     CDS             complement(13587..15359)
                     /locus_tag="VEL74_20450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64965.1"
                     /translation="MPRLAALLLPLTLLLSDAPDARQLMARSTPAEMERRLEADRSLV
                     PSTLGILATALGGDDPALVKETGDYLAAAARSRSRRLGAAGRWQPGQVEDLLVLQLVQ
                     PERFVTDAAFRQRVLDLLPRALDPAVPPALRDGLLAELNQVRGVDFAASERVESAWGA
                     VPRRSGERRTAYPSPAPRFGHDTGGALAASVYSLSSTFFSAEEATSFLAAVHEAAPER
                     RLLVLTDPPLQRALAGSPGVRLLPTYGRPYSPWPRDPFSLAREPGGRLLVLLRPNTQP
                     GREEDLFLGRELVQNLPGDLDKAWGQVHWAEAPVPFHNGQILLTPDAVWLSLHSLEPH
                     ILQILGLSRVPVESFGTAAGIDRYQEAAGKAARELAAFYGRPVRFVHPLAGPGTGDIQ
                     ARRRLLGTLGGGAGYDLDSLLTLLPRPDGGLTALVADVSEGRRLLAALPGDDWLSLER
                     GYGLAPAAGSGPALRERVAAAQETAEASRLDGFLDLVAEHLKKEGLQVRRLPLLAMPV
                     ALLRDRAGLEHDTFLLTWNNVVVETRGDLRRAEGFSSLLPTGDRDARAAFAAAGVRLD
                     LFPPLVRSVVLNGGYRCASNHIRG"
     gene            complement(15378..>17985)
                     /locus_tag="VEL74_20455"
     CDS             complement(15378..>17985)
                     /locus_tag="VEL74_20455"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYG64966.1"
                     /translation="ALAIAETPRDLLRALAEAGSGGSAAFLADPEILEAGEAAPDRTQ
                     TLVEVLEWHARRHPGRRHVLFHGDGEPEELTYGELLRRARAAAQGIRAGGLERGQAVG
                     LMLPSGLDYFVAFFGVLLAGGVPVPLYPPARRSQIEDHLRRQAGILATAQAGLLITFG
                     DVLPLARLLRAQVPEVRRVTTVAELSTAPRASSPAGHPEEPAPPQPEDVAFLQFTSGS
                     TGNPKGVILTHANLLANLRALGQVAEIRPDDVVVSWLPLYHDMGLIGAWMGSLYFGLP
                     LILMSPLAFLARPSRWLQAIHHHRGTISAAPNFAYELCAAKIPDEELQGLDLSSWRTA
                     FNGAEPVSPEAIRRFTERFAPYGFQEGMMTPVYGLAENSLAVTFQVPGRPPRIDAVRR
                     EPFQRGDRAEPAAPGDPAALHFVSCGPPIPTVQVRIVDGTGNEAGERRVGRLQFRSPS
                     ATSGYFRNPEATRRLFHGDWLDSGDLAYIAEGELYLTGRVKDMIIRAGRNLYPQEIEE
                     AVGAIPGIRKGCVAAFGSADPATGTERLVILAETRERDEAALERLRQAVQDTAVDLLG
                     TPADDVVLAPPQTVLKTSSGKIRRQASRELYEGGRIGKGGTALWWQLVRLAGSGLSTR
                     LSRRARVLGDYLYAARVYFVVALVALPAWAAMTLTPGTSRRRRLARRAARLLARAGGM
                     FPRVHGLLDDDGPRLLVANHQSYLDGILLTAALPARFAYVVKRELENAPGAGGFLRRL
                     GTVFVERFDPAQSAGESRKVLDALRAGDSVVIFPEGTFQRQPGLLPFRMGAFLVAAEA
                     GVPVVPVTIHGTRSMLRDEGFFFRRGTPQVTVGPALHPEGTGWDAAVCLRDAARREIL
                     SHCGEPDLGAGG"
BASE COUNT         3124 a         5653 c         6130 g         3078 t
ORIGIN      
        1 gaatctgcgg gacgcgtaca gccggttcgt ggctgccttc cgggacgccg ccgagaaact
       61 gaaggccggc gaccggacgg tggcgttccc ggaggggagc ttccccccgg cagcgccctt
      121 cgtgcggccg gccttcacgt agccgagggc cgacctgggc cgcagacccg ccggcgggtg
      181 agcgccggcg acctgagtag gggagaggtg tgcctgctcg agccgttact gcccaacgag
      241 cgcccctgaa agagccacct agagtgcctc gagcctctcc tgattcgctc tcaatcgacc
      301 ttcctggcct cctgaggccg tctccggcgt cctcttacgc ttgatcagcg gcagaggagc
      361 agcgaatggg gattaatcca tcgttctctt cacttcgagt gggtggcacc acatcccact
      421 gcacctcacc tctctgcagc acctcattct cgtgcacatc attctcctct cctctcctct
      481 ctcattctcc tctcccttta tcccccccgc taatcgtcgt gcagcagtac gtcttcgtgc
      541 tggcctaatt tgattgcaag agcctgaata ttatcaataa cccgtccccc ttcactgagg
      601 cttagctgat ggatctgaag tgtaggcagg ttggggtcac tgaaggctcg gtcacccggg
      661 taggatacag accccgcact tgagcgccct tgaaagagct gtggaattaa gccatttctg
      721 agagagcgct gccgcacgcg tcctccatcc atcaaataca ctttgcagag tgcattctca
      781 tcgcgctcta agagggtctt cagattgcaa tttgcgacgc agaggccaag cgcatcatcc
      841 tcataaagat atgagagtgg aaccaaaacg cgttcgaaga attccctgag ggctacaaat
      901 gcgtagtgat gcctcgggta ggtctcgtgc tttcggaaat caaggccctt caattggccc
      961 aagaaccgat tgttgtgcac tccgtcttca ggagaaaggt ggggcgcttt tggtgcaaac
     1021 cagccatcgt caagagtcgc ccgcatccac gggttcagta ggacatttcg acgtgtcggc
     1081 gctagcctgc tatccatata gaaaaggcgc cgccactctt gaagaggatg gcctcgatgc
     1141 tctgcaagac gtttacgcat gtcctgctca tgggtgacat atgcctcgta gattcttcgg
     1201 acatccgggt gtagataaac gcggcaatag ccaaggtaat ctcgatggta tccaaaaaat
     1261 cgcgctcgct gttgaatcgt gtcagcggtc caaccacccg gagacctggg catgtacgtc
     1321 acggtcagcc ctttcaccgt gtagcctctc cccaacttct ctccgcccac aagcacgtgc
     1381 gagtagccat tctgccatcg gacttcgcgg ccgtctacag aattgacggg tgtgatggta
     1441 gtctggttaa tagccaccgg tagttttgct tggatttgct cgaatggagg aaggcgttgg
     1501 caggtacgct ccaggtcttc gtatgccctt ttgaactctc gaagtagctc ctcacggtca
     1561 ggatcagttt cctctagcaa cacttcgtgc caatcgtgct tcaggcgatt agcccaagtg
     1621 tagtaggagg tgtgagtagc agtcgctttg gatgggtgaa taagcatgga gcggtgcttc
     1681 tggccgcctt caaggcgacc cgcggcaact cctacgaaaa agacctgcat tgcgcgtaaa
     1741 aggctctgcg gtggctctaa gggtagttgg ccatcgcggt agatttctga ttgcggaatc
     1801 aactccacaa aaagagagtc tcgcgcaaag aaatcccgtc cgccggtgta ggactcgcct
     1861 ggagaaacaa gctctgcgaa gtcggcggat agtgagtcaa tgcgcgtgat tagtagaggg
     1921 gcctgtggag tagctgtgta ctgtaggtac gtgtggcgtg gaagtgctcg tctgagttcc
     1981 tcaatgtgcc tgtaggttga actcgcgatt ggatcgttag gccgagtatt aagactcgcc
     2041 tgatccgctt cgtcatcgaa gatgatggcc ggaaagttac ccaggcctgc cttctcgaga
     2101 agttctgata atttatcaag gtgggagtaa ttcttcatta cgcttatgaa gagcgtccgg
     2161 cgatcttcgt cttcgtaatg ctcgctcctc cattcctgcg caagcgctcg gagatcatcc
     2221 ctctcagtct cgggccgagg attggttagc ataagccatt tccagtccgt cgccgctgcg
     2281 cgtagatgtg tttcaagtct atctcggttc tgggtcacga ggttctcggt cgtgccggcg
     2341 atcaggacga tgactcgata tctgttgtct cgggcgagag ctgaaaccgc tgtcatggag
     2401 gctgtttttc cgctctggac gtagccacaa accaggccag tgcgcacggg tgtagattca
     2461 gaaggagggg tacattgcga tagaacctgg tgcgcttctc gaattacccg atcggcattt
     2521 tcttgattga gttcacgaat tccaacccag tgctcgcgca agcgagctgc agtctcccct
     2581 atgataggtt gccatcttgg cctaatagat tgttcactct cgcttgccat tcggcttctc
     2641 tcccttttgg ataggcccgg aaagggcgga tcgaagtatt tggttcaggt ttcggcgcaa
     2701 cgttcctgct tgccgaaccc ccacctcccg agccgtgact tctgcgattg ctaagccggc
     2761 agcgaggcgg agaaagggga caatctcgtc cccatccaga gaaatgaagc gaaccataaa
     2821 ggggtgagca agattgacgc gaatgtgaat ccgccatgta tcggcgcccg gttggctctc
     2881 cgcaatgtcg taccaggact cgcgggttag ttcagagatc acctcaagag tcacaatcca
     2941 gcgacgttgc gcatgctcta gggcaagttc aacctgcacg ctgcgactag aaggctcaga
     3001 gactgcatcg aggtccgaag ccaaggggct ctcatcgggg tcagcgtgta tctgggcgtc
     3061 aacaatcggt ggaagatgtt gagcaatgac ctgggttgtg tctagggccg cctctgctac
     3121 gacgtcgcgg tcggtggtgg cgcgcgcccg atagttagct gcctgatcaa gtagaggaag
     3181 cggctgggca tccaacacac tcttaagcca agcgagtatg tcctcctccc attcgtccca
     3241 ttggattcca tccttggtgt ggcttaccga gaagccatcg acctcgaact cccctacaag
     3301 cctctggtag atgaatttgt taggtttgcc gaaaatctct tctggtcggt atccctctcc
     3361 gtggctaccc tgaataagcc gtcgacgccg gaagatagaa aatccggcgt tcgcgacgct
     3421 tgctcgtgcc aggattgctg cccagccact gactctatgt ccatcgccca gatctaaatt
     3481 aatctccttg cgccaagtta cggggctgct cgtgggggct gcatgatagg gagtgcaaag
     3541 gatcgcagga ttctcatact tgaggtcgtc atcgttcacg cgtagctcga tcaatccgtc
     3601 tctcaggaaa agcctataga tgctccggag gtgctccttg atcttccaaa tggttcggtg
     3661 cttcggtcgc acattaagat cgctgaggag cagagttgta tagtgatcgg tgagttctgc
     3721 cttccgctcg atgggctcga gttcttcgcg gccccttgtg acgatctcag gtatgtcgaa
     3781 agttatggtt ctctcgactg cctcccccaa ggcggtggtg cgaacggacc atttacgcgc
     3841 gaaccaactc gaggccgctt tcattccgag accgaactca gacaggccag atctgtctgg
     3901 aggaggagag gccggccgga aagcacgagc gtaatcccgc tgatgaatac cagctgcggt
     3961 atccctgatc tcaatgtaat cttcgctcac atcgatctgt acagaaagac ggtacgtccc
     4021 gccgcttacc ctttgcaggg cctccatatt cgccaaggca ctttgaagcg agttgtcgac
     4081 aaactcggcc aatgcatacc acggcttata gtttagccgt gcgaaggctg cgtacatcgc
     4141 gacctcggga cgaatagcaa ccaatctaag tctcctgcga gaagggagtg gctacgtcaa
     4201 gccatgtacg tcttgggccc agtccggtac ctggatgagg tggataggaa cccctgaggt
     4261 gcaggtactc actcggtaca gattgatggc gctgtgcggg cgggcaatct ctacgtcggg
     4321 ttggaatcaa ggtgagacgg cgttctggaa tgggatcgct taggaactgc cgtcgcattt
     4381 cgaggcctaa gagttcaccc aaggcgcagg ggacagcatt accgatctgc cgatgggctg
     4441 aacgcctgtt tccaatgaag tagtagccgc gcgggaatgt ctgaagacgg gcaagttcct
     4501 caattgacag cagcctgttg cgccagtgaa aggggccagt cgcagggcct ggggcggctt
     4561 ggatcgtcca ggagggtctc ctcttagcaa gcttaagcag gaatgaccaa tatcgggttc
     4621 tccatccgaa aagtggttcg ccgcctcctt cggatgtatg ccaaaggtag ttctgtcctt
     4681 caggtatgct ccttagaagg ccggcccact tccccgccgg attaagctca gccggccaca
     4741 ctttttcatc gagatctcca atggcatccc aggccgtcgt gtacggcttc cgtctcgggg
     4801 ccagtggact cacttcgtca tcgggagcat gtgtcggcgt tgggaaacga aattggtttc
     4861 cctcccgcgc gccgataacg aaaacgcgct cccgaagctg cggtacgccg aaatcagcgc
     4921 cgttaatttt ttttaccgca atcgtgtagc tcgtcccgca cgcttgattg atctgctcga
     4981 tcgcctgctc gatcaggtgc aggccctcac tcttgttttg gtagaccaag ccaggcacgt
     5041 tttcgagaag aaatgttcta ggtaaggtgt cgcgtaggac ccgaaggtac gcagccaatg
     5101 tgccggcacg gtgatcatcg aggcgcaggg tgtcgcctga tacccaatag ccggacttcg
     5161 agaacggctg acagggagga ccgccgatca gtgcgtctgg ttctcctgat ttcaggcccg
     5221 ctttgtcgag gagctccttc gaggagacag tatgaatatc tcggtcaatc accggccagg
     5281 accggttgtg ccgcaaagtg gccacggctt cggcgtcgag ttcaaccgct acggccgtct
     5341 cgaatcccgc agcctcaaac ccaagatcga ggcctcccgc tcccgtgtag agactaatga
     5401 gcttgcgcat ctaggccctc ttctcctgga gcgcctcgat taccttctga gtcactcctg
     5461 cgggattgga cctgatctcg gactcccaaa agcggagaac ggtccacccc atttcccgca
     5521 ggcgcagagt gttctgttgg tcccgctctc ggttcctttg aatcttggcg agccagtagg
     5581 cggagttcgc accctggccg agcttttggc tccggctttc ccaatctcgg ccatgccaaa
     5641 agtcaccgtc gcagaagacc gcgagcttcg cgcgtgtgaa aacgacgtca ggccgtccgg
     5701 ggaggtccgg caggtgtttt cggtacctgc agccggcagc ccacaaggag cgtcggagca
     5761 tgacctcaca gcgagtatcg gtcttcttgc tgctgccgcg cgcggcagtc gtggcgcgcg
     5821 gagaggagga gcgaagtccc ttgaagctcg cccctgtttg ggacggagga ggggcttcgg
     5881 accttgagct actcatggag ggaatcctaa cccagagagc cctcctctgg agcgtcgctc
     5941 tactacgtct tgtgctctgg cgtaatccgg ccactgtttg tagtacctcc gaggctcaag
     6001 ggagagcccc ggcggccggg gttcccccgg ccgccgggta ctcctactga cctactcccg
     6061 cacccgtccg ctcaggatcg ctcccgcccg ctcgaggatc acggggccgg cggcctcgaa
     6121 gaggctcgcc agggcttcgg gggacctcag gaggcactcg acgacgtgga gggcgtcgat
     6181 cagtttgtcg tcgaagacgg cgaggtggcc gatgacctct cctccggtct cgacggcgta
     6241 gccgtcgggg gtgggttcct tgtggagacg gtagagcggg tcccggccgg tggcggggat
     6301 cagggccgag atcaggaggg cgtggtacca gtcgccgaag atcgcgatgg cccggtcgcc
     6361 tcgcgacgcg tcctggccgg tcgcgtagac gccgtagaag gtcgcgggga tctcctccac
     6421 ccgccagggg cccgcctttg aggcctccgc cgacacggac ggcgacggct cgtcccgctg
     6481 gcggagctgc tgcaggaact cgtggaggaa aatgttggac ggacgccggg tgctaccatc
     6541 atcgttgctt tgcatgggac tcagctccct tgtgaagtag gctcgggggc gaggtgttga
     6601 ctcacctccg ggccgctgtt cagtgggtcc gccgaaggcc ggacgatccg gctccagcgt
     6661 aaaaacaacg caagctgcaa agacttctac gacgggtacg gctggtcgtc gattcgttcg
     6721 cttgtgggac ctccttcccg gggagcgcta cggcttcgtg ccggagcgtt aggagaatta
     6781 taggaaaggg gagcaggtaa atcaagcgga aggggtgggg agagttggag aaagaaaacc
     6841 cccctcctga cctcccccgc tccctaccgc ccacccaccc agggggggag gcaccacctc
     6901 ccgaaagagg gggagttttg ggtcggtcgc tggtgattag tctaaatgag caggaattta
     6961 ggctaagttt gttgacgact aggttagttg gtgtagaatg tagggcaaga ggtaggcact
     7021 gaccctagga gggatcgatc atgcgtccga ccgaaccgag ccgagccgag gcgctcgagg
     7081 agatgacacc agagaagctg gaggagatgg cgcggccagc tccccgggtc tcgcatgcgg
     7141 ggctgcgcga ctttctggag atcccctacg accagttgga agagatgaac ctcgaggcca
     7201 aggaggagcg gctggctcgg cggcccgcgg acgtggtccg cgagcagcgg atgcgctatc
     7261 tcaacgacga gaagcgcatc aaggcggtca ccgtgtgctt caccgacctc gaaggccggc
     7321 tgcacatgct ggactatgac aagaagttcc tgctcaagtc ggcggacaat ctgaccttcg
     7381 acggctcgtc gatccgcggc ttctcgcggc agcaggagtc ggacctgcgg ctggcggtcg
     7441 actggccggc cttctactgg ctgccgtcgg acatcttcgg ccccggcaag gtgctggcct
     7501 tcggcgaggt ccacgagcgc gacggcagcc tgtacggcgc cgacatgcgg gctatgctca
     7561 aggggtacac cgacaagctg tactccaggg acggcatggt cgcccacgcc gccaacgaga
     7621 tcgagggctt cctcttccgc ggacgcgacg ccgagcgctg cttccaggag accggccgct
     7681 tcgagttcat ctccacgggc ggctattacc actcgctgcc gggcgattcc ctgcggcagt
     7741 tcatcgacac cgccgccgag gtgcagcggg ccatgggctt cgccaacgag aaggaccatc
     7801 cggaggtggc gccctcgcag ttcgagatga actacagcta taccgaggcc tcggtggccg
     7861 ccgaccaggt gcagctctac aagctgctct cacggcaggt cgcggcgcgg ctggggatga
     7921 cggcgagctt cctgcccaag ccggtcaccg gcgtcaacgg ctcgggcatg cacaccaacc
     7981 tctcggtgac ccgccagtcg accaatctgt tctacgacaa ggacggcaag gatggtctct
     8041 cggccatggg ctgggacttc atcaaccgca tcctggccag cgccacggac atctgcctgg
     8101 tgctcaactc gagcgtcaac tcgtaccgcc gcctcgatcc ccacttcgag gcgcccaacc
     8161 agatcaaggc ctcggccatc gaccgcggct ccatggtgcg catccccctc ggcaacgagc
     8221 gctcggcgcg cgtcgaggtg cgctccatcg ctcccgactc caacccgtac ctggcgatct
     8281 acaccctgct caggaccggg ctcgaaggtc cccagagcga ggaggacggg gataagaggc
     8341 cgcgcacccg cttcctgccg gacaacatct acgacgccat ccggctgttc aaggggagcc
     8401 ggtttatgca ggaggtgatg ggagaggacg tgcacgccaa atacgccgag atcaagctcg
     8461 cccaggccga acgctgcccc aaggccctcg gcaccctcat caaggcctct gagattcagt
     8521 tccaccacga ggtcacgaac cagtacctct ggtcgcagtt ctaggcgaca gatctcggca
     8581 tctcgtttct ccgcatggcg ggaggatccc tcctcccgcc atggcaggga cgcctgaccc
     8641 cctccagggc cgcgctctac aagagcctgc gtccggctcc tctatacgct ctcaatcttc
     8701 ctgaacagtg ttgaaggtgg cgagcccttc cgaatcggcg gctccgagga gaaggcggtc
     8761 ggagcaggcg aaggtgatct ccaatccttc ctgatccaag agcagggcac ttgcgaggtg
     8821 tacagcgtcg gcgcctcgga gagggtggcg atcgcagagt ccgggtatga gagcgagaac
     8881 ctcggctccg accggcatct gcgtgatccc ttcccaatcc tccgcgaagc gacgccggag
     8941 ctgctcgagc tcggaggcga gaaggagccc ttcgcggcta cggcgagcga aggtcgcatg
     9001 gatctcggcg aacgccaggc gtgaggcggc taccgagctc ccctgcgccg ccgcctcgcg
     9061 cattcgttcg gagcccggtt ccgcgatata gagtttgacc aggacgctgc tgtcgacgaa
     9121 gaggatcaac gccgatcctc aatgatcatc tcggacgcca gcttgccgcg gctcgggacg
     9181 ctggggccca ggaatgtgct ccgcgtccgg ttgggcagtg ccaccagacc acgggccgcc
     9241 agctcggcca agcgagcctc ttcgtcgacc tcgcggagct gggacagcgg gaccaatgct
     9301 gccaccggtt ggccgtctcg cagcacgacg atctgctcac ccgcttcggc tcgttgcaag
     9361 tagctggaga gctggtcttt caggactcgg gtgctcacgg tgggcatcgg ccacctcctg
     9421 cggccattgt agccgcacgg agcccgctac tggaaggctt cggcgagggg atcgcgagca
     9481 cggggaagtc gctccttctc gggccggaag aggttgccac ttccggcggc cgagaaggag
     9541 agggagacgg ggcctggcct aggccagcag gcggagcagc tcgcgctggg gctcgccgcc
     9601 ggtgatctcg gggccctcgg gggtgagggc gacgttgatc tcgctgcgga cgccgaagtc
     9661 gccggtcagg tagatgccgg gctcgatgga gaagccggtc atcggcagga gctgccgggt
     9721 gtcgtgggtc tccaggttgt ccatgttggc gccctggcca tggtcggtag tgccgatcga
     9781 gtggccggtg cggtggatga agcgctcgcc gtagccggcc tggcggatga aggcgcgggc
     9841 ggcgtcgtcc acctcgaagc cgcggaccgg ccgcagcgga tactgcgagc ggaccagctc
     9901 ccaggcggcg tcgcgggcgc cggcgacgat ccggaagatc tcggcgtgcc gctcctgggg
     9961 cgtcaccgag cagacgccgc accaggtgat gtcggcgtag acgcttcccg cggccttctc
    10021 cttggcccac aggtcgatca gcaggaagtc gccgcggcgg atcggcgagg agtggtccgg
    10081 gcccggctgg tagtgcgggt cggcgctgtg ggcgttgacg ccgatgatcg gggcggcgtc
    10141 ggtgatcagg cccgcgcggt cgaaggactc catgatccac tgctggacca tgtactcgtc
    10201 cacctcgtcg ccgcggcgga ggatgtcgcc cacgcgggtg aaggcgtcct tgacgatgga
    10261 gtgcaggtgg acgttggcgc gccggtggcc ggcgagctgc tcgtcgctcc aggtggcggc
    10321 gaagcgctgc gcgaggtcgg cggcggagac caggtccacc cccagctcgc gcagcagctc
    10381 ggcggtgccg gcgtccaggc gcgagatggt ggggatctcg ttgcgcgggc tgtagtgggc
    10441 ggcgatccgc tccacgccgg agaccagctt ggcgagctgc tcccggttct cctgccaggt
    10501 ggtgtactcc gccttcgagc ccggcaggtg gtcgagcatc gactcctcca ggccgtgcac
    10561 gagcttccgc ggctcgccct ggcgggggat caggtagtag cagcggcggc tgaccagctt
    10621 cccctcgccc acgaggccca ggagatccag gctgatgggg tcgttgaact gaaagcacat
    10681 gaagaaccat ccatccagtc cggcctcggc gagggcgtcc tggatctcgg ggatccgttc
    10741 ggcgagcatt cctctcctcc tcttgctcga ggggcatcag gcccccgggc agggtcaaca
    10801 atgaaaacga cagttgaagt ccgccaatcc tatcagagac ttctagaatg aggtgcggcg
    10861 taccggctcg gatgcgcccc gatcctctca tgagagcttt ttactgcgac gagttcgtcc
    10921 tcccgctccc cgaggggcac cggtttccga tggagaagta ccgtctcctg cgccaggggg
    10981 tgctcgagcg cggcctggtg gcgccggagg acctgcgggt ccccgtcgcc gccacggacg
    11041 aggagatcct gcgcgtccac gaaccggtct acctggagcg ggtaggcgcc ggcacgctgg
    11101 agcgcgccga ggtgcgccgc atcggcttcc cctggtcgcc ggcgatggtg gagcgttccc
    11161 gccgctcggc cggcggcacg ctggccgcct gccgcgaggc gctggagaac ggcagcgccg
    11221 tcaacctcgc cggcggcacc caccatgcct tccgcgaccg cggcgagggg tactgcgtgt
    11281 tcaacgacgc cgccatcgcc gcccgcgcca tgcaggccga ggggcgggtg gagcgggtgg
    11341 tggtcctcga ctgcgacgtc caccagggca acggcaccgc cgccatcttc caggacgacc
    11401 cgacggtctt caccttctcc atccacggcc gccacaattt ccccttccac aaggagagga
    11461 gcgacctcga cgtggagctg gaggacggca ccggcgacga ggagtacctg gcaaacctct
    11521 cgcaggggtt ggagcgggtg ttcgcggcgg gggcggcgga ggccgggctg gcgatctacg
    11581 tgtcgggcgc cgatccgcac cagggggacc ggctggggcg cctcgccctc tcccgtgccg
    11641 gcctcctcgc ccgcgaccgg ctggtcttcg aggcctgccg gcggtgcggg ctgccggtgg
    11701 ccgtggtgat ggccggcggc tacaaccggg aggtgagcga gacggtggag atccatctcc
    11761 agacggtcgc ggaggcgcgg cgccactttg gcatgtggac acgggtttgg gattagagac
    11821 ggccctccaa ctgttttcat gtgcgggacg agtgggtcta aagcacggag cgccgaggta
    11881 gaatacccgg ccgtcggaaa cttcaacctt tcccaagaaa ggcaggcaag acgcatgatt
    11941 cgcaactatc gcacgctcgt cgtagcgttg ctgctcagtc tgctcgctgc gggctccgtg
    12001 tgggctatcg gcgaaggcag gttcacgggg accgtgatgg atcaggaggg caagccggtc
    12061 gccggcgcca agatcaccgt cacgagcccg ggctcgagct acaagcagga gaagaccacc
    12121 aacgacaagg gcaagttcac catcatggtg gtggacgcga tgcgcaagta cgagatccgg
    12181 atcgagaagg agggctactc gccgatccag ggtccgctgg cgatcaaccc gtcggccccg
    12241 accgaccagg acttcactct tgcggtcgcc ggcccgagcg ccgaggagct ggccgagatc
    12301 aagggcaaga acgacgccgt ggccgccttc aacgagggcg tggccctggt caaggcgaag
    12361 gacttcccgg gtgccgcggg caagttcgag caggcgctga cgctcgatcc gacgatcacc
    12421 cagggccacc aggtgctggc cgacgtctac ttcgagatga agaagtaccc cgaggcgatc
    12481 gcctccgcgg acaagtatct ggcgaccagc cccgcgagca cgaacggcat gtacgtcttg
    12541 aagtacgact cctacctggc catgggcgac aaggcgaagg cctcggccgc cctgcaggag
    12601 atgctgcaga aggagcccgg caaggatgcc gcggtgcgga tcttcaacct gggcgccgag
    12661 gcgcagcgca aggatgacga cgccaacgcc gagaagtacc tgcggcaggc gatcgagctc
    12721 gatcccacgc tggagcgggc ctacgcgtct ctgggcacct actacctgcg ccagaagaag
    12781 tacgacgcct ccctggacat ggccgaccgc ctgctgaagc agaacgccaa cagcgccgag
    12841 gccctgtccc tccgctacga gtcgctcaag gcgctcaagc ggaccgacga ggccaagcag
    12901 gcgatggcgg cgatgcaggc gtccgccggc aaccagaccg ccgaggaggc gttccggcag
    12961 ggcgtggcgc tctacaacgc caacaacgtc gccgaggcgg agaaggcctt cgagcgcgcc
    13021 ctggccaagg acggcaacta cgccaaggcc cactatctcc tcgggctcgt cgaggccagc
    13081 aagggcaaca tggccaaggc ccgggagtac gtccagacgt tcctgaagct ggctcccaac
    13141 gacccccagg ccgcggaggc caaggcggtc ctggccgagc tcaagtaagg ctctccggat
    13201 caccgcgaga aggccgggcg ctccacggag ccccggcctt ttctcttggc ccctcccccc
    13261 cttggacagg ctggcggaag cttccggcgc gccagcggag ttgccggcgc ggtctcggag
    13321 tctccggcac gcggtcagag tttccgatgc gctgccggag cctccggcgt tccagcgcaa
    13381 tctctgacga gccgccggca gcttcggcgg cctgccggag tctccggcgc gccaccgccg
    13441 tcttcggcag gttgccggag cctccgatgc gctgtcagag tctccggcgg gctctcggag
    13501 tttccggcgg gctgtcagag cctgcggcag cccaacgcga tcttcgacaa gccgtcggaa
    13561 gctccgatcc gccgccggag gcttcgctac ccgcggatgt ggttggaggc gcagcggtag
    13621 ccgccgttga ggacgacgct gcgcaccagg ggcgggaaga ggtcgaggcg gacgccggcg
    13681 gcggcgaagg ccgcgcgcgc gtcgcggtcg ccggtgggga ggagcgagga gaacccctcg
    13741 gcgcgccgga ggtccccgcg ggtctcgacc accacgttgt tccaggtgag caggaaggtg
    13801 tcgtgctcga ggccggcgcg gtcgcgcagc agcgccaccg gcatcgccag cagggggagc
    13861 cgccggacct ggagcccttc cttcttcagg tgctccgcca cgagatcgag gaagccgtcc
    13921 aggcgggagg cttcggccgt ctcctgggcc gccgcgaccc tctcgcggag cgccggtcca
    13981 ctgcccgcag ccggcgccag cccgtacccc cgctccaggg agagccagtc atcccccggc
    14041 agcgccgcga gcagccggcg gccctccgag acgtccgcca ccaaggcggt gaggccgccg
    14101 tccgggcgcg gcagcagggt gagcagggag tccaggtcat agccggcgcc gccgccgagg
    14161 gtgccgagca gccggcggcg ggcctggata tcgcccgtgc cgggtccggc cagagggtgg
    14221 acgaagcgca ccggccggcc gtagaaggcc gccagctccc gggcggcctt gcccgccgcc
    14281 tcctggtagc ggtcgatgcc ggcggcggtg ccgaagctct ccaccggcac ccgggagaga
    14341 ccgaggatct gaaggatgtg gggctccagg ctgtgcaggc tcaaccagac ggcgtcgggc
    14401 gtcagcagga tctggccgtt gtggaacggg accggcgcct cggcccagtg gacctggccc
    14461 caggccttgt cgaggtcccc tggcaggttc tgcaccagct cgcggccgag gaagaggtcc
    14521 tcctcgcgtc cgggctgggt gttgggccgc agcaggacga ggaggcggcc gccgggctcg
    14581 cgggcgaggc tgaacgggtc gcgcggccag ggggagtagg ggcggccgta ggtggggagg
    14641 agacggacgc ccgggctgcc ggccagcgcc cgctggagcg gcgggtcggt caaaacgagc
    14701 aggcggcgct ccggagcggc ctcgtggacg gcggccagga aggaggtcgc ctcttcggca
    14761 ctgaagaagg tggaggagag ggagtagacc gaggcggcga gggcccctcc ggtgtcgtgg
    14821 ccgaagcgcg gggccggtga cgggtaggcc gtccggcgct ccccggagcg gcggggcacg
    14881 gcgccccagg cgctctccac cctctcggag gccgcgaagt ccacgccgcg gacctggttc
    14941 agctcggcga gcaggccgtc gcgcagggca ggagggacgg cgggatcgag ggcccggggg
    15001 aggaggtcca gcacccgctg ccggaaggcc gcgtcggtga cgaagcgctc cggctggacg
    15061 agctggagca ccaggaggtc ctccacctgg ccgggctgcc agcggccggc cgcgcccagc
    15121 ctccggctcc gggaacgggc ggcggcggcg aggtagtccc cggtctcctt caccagggcc
    15181 ggatcgtcac cccccagcgc cgtggccagg attcccaggg tggaggggac gagcgaccgg
    15241 tccgcctcca gccgccgctc catctcggcg ggcgtggagc gcgccatgag ctgccgggcg
    15301 tccggcgcgt ccgacaggag cagggtgagg gggaggagaa gggcggcgag acggggcatc
    15361 ggggggtagt gtaggcccta accccccgcg cccagatccg gctcgccgca gtgggagagg
    15421 atctcgcgcc gggcggcgtc gcgcaggcag acggcggcgt cccagccggt tccctccggg
    15481 tgaagagccg ggccgaccgt cacctggggg gtcccgcggc gaaagaagaa gccttcgtcg
    15541 cgcagcatgg agcgggtgcc gtggatcgtt accggcacca cgggcacgcc ggcctcggcg
    15601 gcgaccagga aggctcccat gcggaagggc aggagcccgg gctggcgctg gaaggtcccc
    15661 tcggggaaga tcaccaccga gtcgccggcg cgcagggcgt ccagcacctt gcggctctcc
    15721 ccggcgctct gggcggggtc gaagcgctcg acgaagaccg tccccagccg ccgcaggaag
    15781 ccgccggcgc cgggcgcgtt ttccagctcc cgcttgacca cgtaggcgaa gcgcgccggc
    15841 agcgccgcgg tcaggaggat gccgtcgagg tagctctggt ggttggcgac gaggaggcgc
    15901 gggccatcgt cgtccaggag cccgtggacc ctggggaaca tgccgccggc gcgggcgagg
    15961 agccgggcgg cccggcgggc gaggcggcgc cggcgcgagg tcccgggggt gagcgtcatg
    16021 gcggcccacg cggggagggc caccagggcg acgacgaaat agacccgggc ggcgtagagg
    16081 taatccccga ggacccgggc ccggcgggag aggcgggtcg agaggcccga gccggccagg
    16141 cggacgagct gccaccagag ggccgtgccg cccttgccga tccggccgcc ctcgtagagc
    16201 tcgcggctcg cctgccgccg gatcttgccg ctggaggtct tgagcacggt ctgcggcggc
    16261 gccaggacga cgtcgtcggc cggcgtgccg aggagatcga ccgcggtgtc ctggacggct
    16321 tgccgcaggc gctccagggc ggcttcgtcg cgctcgcggg tctcggcgag gatgacgaga
    16381 cgctcggtgc cggtggcggg atcggcgctg ccgaaggcgg cgacgcaccc cttgcggatg
    16441 ccggggatgg cccccaccgc ctcctcgatc tcctgcgggt agaggttccg tcccgcgcgg
    16501 atgatcatgt ccttcacccg gccggtgagg tagagctcgc cctccgcgat gtaggcgagg
    16561 tcgcccgagt cgagccagtc gccgtggaac aggcgccggg tggcctccgg gttgcggaag
    16621 tagcccgagg tggcggaggg gctcctgaat tgcagccggc ccacccggcg ctcgccggcc
    16681 tcgttgccgg tgccgtccac gatccgcacc tggacggtgg ggatgggtgg gccacaggag
    16741 acgaagtgga gggccgccgg gtcgccgggc gccgccggct ccgcccggtc gccgcgctgg
    16801 aacggctcgc ggcggacggc gtcgatgcgc ggtggccggc cgggcacctg gaaggtgacg
    16861 gccagggagt tctcggccag gccgtagacc ggcgtcatca tcccctcctg gaagccgtag
    16921 ggggcgaagc gctcggtaaa gcgccggatc gcctcggggc tcaccggctc ggcgccgttg
    16981 aacgccgtcc gccaggagga gaggtcgagg ccctggagct cctcgtccgg gatcttggca
    17041 gcgcacagct cgtaggcgaa gttgggcgcc gcggagatgg tcccccggtg gtggtggatg
    17101 gcctggagcc agcgcgacgg ccgggccagg aaggcgaggg gggacatcag gatcagcggc
    17161 aggccgaaat agaggctccc catccaggcg ccgatcaacc ccatgtcgtg atagagcggc
    17221 agccagctca ccaccacgtc gtccggccgg atctccgcca cctggccgag cgcccgcagg
    17281 ttcgccagca ggttggcgtg ggtgaggatc acccccttcg ggttgccggt gcttccggag
    17341 gtgaactgga ggaaggccac gtcctccggc tgcggaggag cgggctcctc cgggtggccg
    17401 gccggagacg acgctctcgg cgccgtggag agctcggcca cggtggtcac gcggcgcacc
    17461 tcgggcacct gggcgcgcag cagacgggcc agggggagga cgtcgccgaa ggtgatgagg
    17521 agcccggcct gggccgtcgc caggatgccg gcctggcggc gcaggtggtc ctcgatctgg
    17581 ctgcgccggg cgggcgggta gagcgggacg gggacgccgc cggcgagaag gacgccgaaa
    17641 aaggcgacga agtagtcgag gccgctggga agcatcagcc ctaccgcctg acccctttcg
    17701 aggccgcccg cccggatgcc ctgggcggcc gcccgggcgc ggcgcagcag ctcgccatag
    17761 gtcagctcct cgggctcgcc gtcgccatgg aagaggacgt gccgccggcc ggggtggcgg
    17821 cgggcgtgcc attccaggac ctcgacgagg gtctgcgtgc ggtcgggagc ggcttcgccc
    17881 gcctcgagga tctcggggtc cgccaggaag gcggccgagc cgccggaccc cgcctcggcc
    17941 agtgcgcgca gcaggtcgcg aggggtctcg gcgatggcca gggcg
//
LOCUS       DASPJI010000730         4378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_103576, whole genome shotgun sequence.
ACCESSION   DASPJI010000730 DASPJI010000000
VERSION     DASPJI010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103576"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VEL74_20460"
     CDS             <1..207
                     /locus_tag="VEL74_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64967.1"
                     /translation="AQILSDLDQLSPEKQKRAAELVHQLVPPPKGVSGRDLLRFAGIL
                     DAESAREMEEAIEEGCERVDLDEW"
     gene            179..586
                     /locus_tag="VEL74_20465"
     CDS             179..586
                     /locus_tag="VEL74_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006198663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG64968.1"
                     /translation="MSGWTSMSGRYLLDTNVVIALFRGDAEVQARYEVVQEAFVPSVT
                     LGELYFGAANSSRQEANTGRVEEFAAFCAVIGTDAVTAQHYGLLKAQLKKKGRPIPEN
                     DLWIAACALQHGLILATRDGHFNDIEGLQLQAW"
     gene            1994..2860
                     /locus_tag="VEL74_20470"
     CDS             1994..2860
                     /locus_tag="VEL74_20470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HYG64969.1"
                     /translation="MTSPYGAQSMVDPLRRVLVKRPDEAFASADPERWNYTARPDLDE
                     ARREHDALAEILRGFGAEVIDHPEPQPERADSLFVFDPVIVTEQGAVILRMGKELRRG
                     EEAALARRLQEIGLPVLGALEGEATAEGGDLLWLDPQTLAVGQGFRTNAEGLRQLREI
                     LSGIGVEVLPVELPYFTGPAACLHLLSLISLVDHDLAVVYPPLLAVPFWKTLRDRGVR
                     LVEVPAEEFPSMGPNVLALAPGRCLMLEGNPVTRRRLEAAGCEVLTYRGREISLKAEG
                     GPTCLTRPIWRD"
     gene            2871..4061
                     /locus_tag="VEL74_20475"
     CDS             2871..4061
                     /locus_tag="VEL74_20475"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYG64970.1"
                     /translation="MHPLGPKRTLNGLTVAVLLVGLLAAGCRGSLPGPSSSAAVPSFA
                     EVDRLLEGFRERGAFPGGVLAAGDEEGLVHLHPFGRLSYEADAPPVTSETLYDLASLT
                     KVVATTTMAMILVDEGRLDLDQPVQELLPGFQGPGKEAVTVRHLLTHSSGLQGIVPLY
                     KEVQGREAFRERVLALDLIYPPGSRSVYSDPGILLLGEILEQAAGQPLAAFVRDRVFA
                     PLGMRETRFRPPPELRSRIAPTEFDPWRGRLVHGEVHDENAFALGGVAPHAGLFGTAG
                     DLARFARMLLNGGVLDGRCIVSRETVELFTRRAGIPDSDRALGWDTKSAEGSSAGSLM
                     SARSFGHTGFTGTSVWIDPERRLFVILLTNRVHPTRESNLIREVRPAVADAVVRALEF
                     RSVD"
     gene            complement(4106..>4378)
                     /locus_tag="VEL74_20480"
     CDS             complement(4106..>4378)
                     /locus_tag="VEL74_20480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64971.1"
                     /translation="VLGGANLPERIKPEQIKPEWYKPEGVKPEWYKPEWIKPEGIKPE
                     LLKERFKPELTKPPFEIDEWFDPVLETLSQDQLKRLSEKVNERLRG"
BASE COUNT          649 a         1485 c         1463 g          781 t
ORIGIN      
        1 gcccagatcc tgagcgacct cgaccaactc tcgccggaga agcagaaacg ggccgccgag
       61 ttggtccatc agttggttcc gcctcccaag ggtgtttccg gccgcgacct tctgcgcttc
      121 gcaggcatac tggatgctga atcggcgcgc gagatggagg aggcgatcga ggaggggtgt
      181 gagcgggtgg acctcgatga gtggtagata tcttctcgac accaacgtcg taatcgccct
      241 atttcgtgga gatgccgagg ttcaggctcg ttacgaggtg gtccaggaag ccttcgtccc
      301 ctcggtcact ctcggagagt tgtactttgg cgccgcgaac tccagccgcc aggaagctaa
      361 cacaggccgg gtcgaggaat ttgctgcatt ttgcgcagtg atcggcacgg atgcagtcac
      421 cgcccagcat tatggtttgc tcaaggcgca gctcaagaag aaagggcggc cgatcccaga
      481 gaatgatctc tggatcgctg catgcgctct ccagcatggc ctcattctgg caactcgcga
      541 cggccacttt aacgatatcg agggtcttca actccaggct tggtagctcc cctgcttgcg
      601 cgtacaggcg gacggcggcc tcgagctcta ccgtctggga gagagcgccg agcgcgggtc
      661 ccggccgttc gcccggttcc agcaccggtg ggaagcgcgg tccggtcaaa agcttggtgc
      721 ttgacctgag aatagaaggt taatagacta agtgtatgac ctctaaactg acatcgatct
      781 tcctcgttgt ctgctgcgtc gcctccgctg tgctcgccca gccggcgaag cctttttctc
      841 cactcgacga ggtcgaggtt ctccgaggat gggtagacgt ccaaacctcg ggcgagatgg
      901 tgacggtgca cgtagggagg ctcagcgggt ctactgatcg tgtcttcaag gtgcagttgg
      961 aggagcaggc cgcgcccgtc tccttccagt ccaatgctgc gcaggtcttc ttctggcgag
     1021 ggaacctcgc cgtgctggcc atcggggagg ggagggctct gcgattctcc atccctggct
     1081 tccaggctcc tccgggaccc gcgaccggca gactcgtacc cggggccgac ctcgacgctc
     1141 tgctggcccg gtatgagctg acgaagatcg acaccgcctc cgcgatcgtt tccggtcaag
     1201 ggccacggcc gcttctcgat ccgcggtggg gagagccggg gagccgttcc aaggtcgacg
     1261 atctgactcc ccctgcctcg ggcggtagcg gtgtgggcgc ctgcggtgtc tcctgcgata
     1321 tctcctgcgg ggatggctcg agctgtagcg ccagttgtgg cgcgaagcgc tgcgcctcct
     1381 gcacctgccc cgcctcctgc acgtgcagtt gaggtctggc aggaggcggc gtatcgcctc
     1441 cagactgatg ttggatctga ccccgggagg aaccctcccc cccggggtct ttccactcta
     1501 accctgtaag agcaatgacc tacatttcgg ggccggtcct gcaccggcaa ctggggctga
     1561 aaccttctcc cgagcagacg gagtcggcat agcacttccc gatcctgtac gccccctggc
     1621 tcaggtagta gcaactggca ttgatggtca gcccctggca attcgagggg ctgcaggacc
     1681 cgcaaggggt gcccgccttc ggggcggggc tcggaaggaa ggctagagac tcctgcggga
     1741 caacgctctc gacgcagaac ggctccgcaa gctcaggcgt cgaggccggg ggagcggccg
     1801 cgggggtgtc tgccggtgcg gcgccggcga gggtggcgcc gaacagcaag cagacggcca
     1861 gcaacaagag ggatcctaat ctcatcaggt ctcctttcgc ggagcagtcg agctccgtct
     1921 tctctaagca aggtgccgga cgccccctct accggaacag ccgcgggcgg cgatctgata
     1981 gcgtcctccc atcatgacgt ccccttacgg tgcccagagc atggtcgacc cgttgcggcg
     2041 ggtcctggtc aagcgcccgg acgaggcgtt cgcgagcgcc gatccggagc gatggaacta
     2101 caccgcccgg cccgacctcg acgaggcccg gcgcgagcac gacgcgctgg ccgagatcct
     2161 ccgcggcttc ggcgccgagg tgatcgacca tcccgagccc cagccggagc gggcggactc
     2221 cctcttcgtg ttcgacccgg tgatcgtgac cgagcagggc gccgtgatcc tgcgcatggg
     2281 caaggagctc cgccggggcg aggaggcggc gctggcccgg cggctccagg agatcggcct
     2341 cccggtcctc ggcgcgctcg agggcgaggc gacggccgaa gggggggacc tcctctggct
     2401 cgatccccag acgctggccg tgggccaggg cttccgcacc aatgccgagg ggctccgcca
     2461 gctccgggag atcctgtccg ggatcggcgt ggaggtgctg cccgtcgagc ttccctattt
     2521 caccggcccc gcggcctgcc tccacctgct gtcgctgatc agcctggtgg atcacgacct
     2581 cgcggtggtc tacccacccc tcctcgccgt tcccttctgg aagacgctgc gggaccgcgg
     2641 cgtccggctg gtcgaggtcc ccgcggagga gtttccctcg atggggccga acgtcctggc
     2701 gctcgcgccc ggccggtgcc tgatgctgga aggcaatccc gtcacccgcc gccgtctgga
     2761 ggcggcgggc tgcgaggtcc tcacctacag gggccgggag atctccctca aagccgaggg
     2821 cggcccgacc tgcctgacgc ggccgatctg gcgcgattga tcccgtcgcc atgcaccccc
     2881 tcggtccaaa gcgcacgctg aatgggctga cggtcgccgt cctgctcgtg ggcctgctcg
     2941 ccgcgggctg ccgcgggtcg ctgcccggtc cttcgtcctc tgccgcggtt ccttccttcg
     3001 ctgaggtcga ccgcctcctc gaaggcttcc gggagcgtgg ggcttttcca ggaggggtac
     3061 tcgcggccgg cgatgaggag ggcctggtcc atctgcaccc cttcggtcgg ctcagctacg
     3121 aagcggacgc gccgccggtc acctccgaaa cgctgtatga cctcgccagc ctcaccaagg
     3181 tggtcgccac cacgaccatg gcgatgatcc tcgtcgacga ggggcggctc gatctggacc
     3241 agccggtgca ggagctgctg cccggattcc aggggccggg caaggaggcc gtgacggtgc
     3301 gccacctgct cacccattcc tcgggcctcc agggcatcgt gcctctctac aaggaggtcc
     3361 aggggcggga ggcctttcgc gaacgggtcc tggccctgga tctgatctat ccgccgggga
     3421 gccgctccgt ctacagcgat cccgggatcc tcctgctggg cgagatcctg gagcaggccg
     3481 cggggcagcc gttggcggcc ttcgtgcgcg accgggtctt cgcgccgctg gggatgcggg
     3541 aaacccggtt ccggccgccg cccgagctgc ggtcgcggat cgctccgacg gaattcgacc
     3601 cctggcgcgg ccggctggtc cacggcgagg tgcacgacga gaacgccttc gccctgggcg
     3661 gcgtcgcccc ccacgccggg ctcttcggca cggccggcga tctggcccgc ttcgcccgga
     3721 tgctcctgaa cggcggcgtt ctcgacggac gctgcatcgt ctcccgcgag accgtggagc
     3781 tgttcacccg cagggccggg attccggact ccgaccgcgc cctcggctgg gacacgaaat
     3841 ccgccgaggg ctcgtccgcc ggctcgctga tgtccgcgcg gtcgttcggc cacaccggct
     3901 tcaccggcac ctcggtctgg atcgatcccg agcgccggct cttcgtcatc ctcctcacca
     3961 accgggtgca ccccacgcgc gagagcaacc tcattcgcga agtgcgcccg gcggtggcgg
     4021 acgcggtggt gcgggcgctg gaatttcgat cggtggatta aaaagaggcg cccggacgtc
     4081 gccgcccggg cgccttgagg gcggcctagc cccggagtct ctcgttgacc ttctcggaga
     4141 gcctcttgag ctggtcctgg gaaagcgttt cgaggaccgg atcgaaccac tcgtcgatct
     4201 cgaacggcgg cttggtgagc tcgggcttga accgctcctt gaggagctcc ggcttgatgc
     4261 cctcgggctt gatccattcc ggcttgtacc actccggctt caccccctcc ggcttgtacc
     4321 actccggctt gatctgctcc ggcttgatcc gctccggcag gttcgccccg cccagcac
//
LOCUS       DASPJI010000731         3332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_46813, whole genome shotgun sequence.
ACCESSION   DASPJI010000731 DASPJI010000000
VERSION     DASPJI010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46813"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="VEL74_20485"
     CDS             <1..1782
                     /locus_tag="VEL74_20485"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYG64972.1"
                     /translation="AGIQEQDVVIAVDGEAVGSSEELAVAVDAHAPGDTVTVEVVRGG
                     GSDLWGSSALGGVIQLLTATPGEPAASAVLEGGTHGQLHAGAGVSRRFGRFGASLDAS
                     RVEAGYPVVRRGQRGAIDIDATSEATALYGKLDAAVSPATTLSLRGNLFDEERGNGTP
                     LTSNGTFGGYLSAGVAHSAGERGEWAASLTAQRQSFDSTFSSQAPDRASEVPSLDQFD
                     VTAESLAAAAQWTGSVTAGLDARWTGAENAEDFLFSAETGRFQRRRRAGGEQLQGGIY
                     LQKDVGLGERWRFTAGGRVDAWSHRDGFRVERTLDGGTVLLAEEFPDRDDVAVSPRLS
                     LVYAPRQALRVRGALYQAFRAPTINELYRPFRVRNDITAANPALDPETLTGAELGLDL
                     GNPRSSARLTAFWNRLEDPVANVTLGLGPGTVGPCGFVPAGGSCRQRQNLGSVRVTGL
                     EAEAEHRPAPAWSVSANYLLSHPEIRDSPREPALEGNRLAQVPEHQVVVRLSHSDPEV
                     LAGSLQVRYTGEQFEDDLNTLELDDAVVADLLLRRRLRPGLDLFVAVENLLDETVEAG
                     LTADGLVSIGAPRLIRGGLRLLLGGTR"
     gene            2050..>3332
                     /locus_tag="VEL74_20490"
     CDS             2050..>3332
                     /locus_tag="VEL74_20490"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYG64973.1"
                     /translation="MTRPPLPVEEVLPDLLAAVQDRGSAVLVAPTGAGKTTRVPPALL
                     EAGIGPIGDGRRIVMLEPRRIAARAAARRMAEEGGWKLGEEVGYQVRFERRAGPGTRI
                     LVVTEGILVQMLQGDPFLEDVGAVVFDEIHERSLHTDLSLAMARRVQREVRPDLVLIA
                     MSATVDPGPIAAFLGDCPVLESRGRLFPVEISYLKDGAPGADTRPLPGLVAAGVRQML
                     EVTPGDLLVFLPGVGEIQRSAEALEPLAAERGLAVLSLYGDLPAERQDEVLRPLSRRK
                     VVLATNVAETSLTIDGVSAVVDSGLVRRLRFDPATGLDRLELGKVSRASADQRAGRAG
                     RQGPGHCLRLWGAWEHGALPEREAPEIGRVDLAAPALQLLSWGETDLAAFGWFEPPDP
                     AALANALRLLRHLGATDDHGVTEMGRTLARLPVHPR"
BASE COUNT          396 a         1290 c         1215 g          431 t
ORIGIN      
        1 gccggcatcc aggagcagga cgtggtgatc gccgtggacg gcgaggccgt cggcagctcc
       61 gaggagctgg ccgtggccgt cgacgcgcac gccccgggcg acaccgtcac cgtcgaggtc
      121 gtccgcggcg gcggctcgga cctgtggggg agctcggcgc tcggcggcgt gatccagctc
      181 ctgaccgcca ccccgggcga gccggcggcc agcgccgtcc tggagggggg cactcacggc
      241 cagctccacg ccggcgccgg tgtcagtcgc cgcttcggcc gcttcggggc ctccctcgac
      301 gccagccgcg tcgaggccgg ctatccggtg gtccgccgcg gccagcgcgg ggcgatcgac
      361 atcgacgcca cctcggaggc gacggcgctc tacggcaagc tcgacgccgc ggtctcgccg
      421 gcgacgaccc tgtccttgcg cggcaacctg ttcgacgagg agcgcggcaa cggcacgccg
      481 ctgacgtcga acggcacctt cggcggctac ctgagcgccg gggtcgccca ctcggcgggg
      541 gagcgcggcg agtgggcggc ctcgctgacc gcccagcggc agagcttcga cagcaccttc
      601 tccagccagg cgccggaccg cgcctcggag gtcccctccc tcgaccagtt cgacgtcacc
      661 gccgagagcc tcgcggcggc ggcgcagtgg acggggtccg tgaccgccgg gctcgacgcc
      721 cgctggaccg gcgccgagaa cgccgaggac ttcctcttct ccgccgagac gggccgcttc
      781 cagcgccgcc gccgggccgg gggcgagcag ctccaggggg ggatctatct tcagaaggac
      841 gtcggcctcg gcgagaggtg gcgcttcacg gccggcgggc gggtcgacgc ctggagccac
      901 cgggacggct tccgggtgga gcggaccctc gacggcggca ccgtgctgct cgccgaggag
      961 ttccccgacc gcgacgacgt cgccgtcagc ccgcggctga gcctggtgta cgccccgcgg
     1021 caggccctgc gggtgcgcgg cgccctctac caggccttcc gagccccgac catcaacgag
     1081 ctgtaccggc cgttccgggt ccgcaacgac atcacggccg ccaacccggc cctggacccg
     1141 gagacgctga ccggcgccga gctcggcctc gacctgggga atccccggtc gagcgcccgc
     1201 ctgaccgcgt tttggaaccg gctggaggac ccggtcgcca acgtcaccct ggggctcggc
     1261 ccggggaccg tcggcccctg cggcttcgtg ccggcggggg ggagctgccg ccagcgccag
     1321 aacctgggat cggtccgcgt caccggcctg gaggcggagg ccgagcaccg tccggcgccc
     1381 gcctggtcgg tctccgccaa ctatctcctg agccacccgg aaatccggga ctccccgcgc
     1441 gagcccgccc tcgagggcaa ccgcctggcc caggtgccgg agcaccaagt ggtggtccgg
     1501 ctcagccact cggaccccga ggtgctggcc ggctcgctcc aggtccgcta caccggcgag
     1561 cagttcgagg acgacctcaa caccctggag ctggacgacg ccgtcgtcgc cgacctcctc
     1621 ctccgccgcc gcctccggcc gggcctggac ctcttcgtcg ccgtggagaa cctcctcgac
     1681 gagacggtcg aagccggcct caccgccgac ggcctggtct ccatcggcgc cccgcggctg
     1741 atccgcggtg gcttgcggct cctgctcggc ggcacccgct aggccggacc tccgcatgtc
     1801 cgatcgcacc cccgtaggcg ccaactccag catcgcgccg ttggggcggg ggcttcctcc
     1861 ccccctggga gggggtgtgg tagggagtgg gggaggaccg aggtgggggg ttgctttcgg
     1921 aagggcgggg ggaggtccgc gtcgagcgcc gcggaaaggc tgcccccctc cttcacctcc
     1981 cccactccct accgcccacc acccaggggg ggaggcacca ccaccccctg cggcctggag
     2041 ctggtgccca tgacccgccc ccctctcccg gtcgaggagg tcctgccgga cctcctcgcc
     2101 gccgtgcagg accgcgggtc ggcggtgctc gtcgcgccca ccggagcggg caagaccacg
     2161 cgggtgcctc ctgccctcct cgaagccggg atcggcccca tcggcgacgg ccggcggatc
     2221 gtcatgctgg agccgcggcg gatcgcggcg cgggcggcgg cgcggcgcat ggcggaggag
     2281 gggggctgga agctgggcga ggaggtgggc taccaggtcc gcttcgagcg ccgggcggga
     2341 cccgggaccc gcatcctcgt ggtcaccgag gggatcctgg tccagatgct ccagggggac
     2401 cccttcctgg aggacgtggg cgccgtggtc ttcgacgaga tccacgagcg cagcctgcac
     2461 accgacctct ccctcgccat ggcgcggcgg gtgcagcggg aggtgcggcc cgatctcgtc
     2521 ctgatcgcca tgtccgccac cgtggacccc ggccccatcg ccgccttcct gggagactgt
     2581 ccggtcctgg agagccgggg ccgcctcttc cccgtggaga tctcctatct gaaggatggc
     2641 gcgcctggcg ccgacacccg gccgctcccc ggactggtgg cggccggcgt ccggcagatg
     2701 ctggaggtga cgcccggcga cctcctggtc ttcctgcccg gcgtggggga gatccagcgc
     2761 tcggccgagg ccctggagcc gctggccgcc gagcgcggcc tcgccgtcct ctccctctac
     2821 ggggacctcc cggcggagcg gcaggacgaa gtgctccggc cgctctcccg gcgcaaggtg
     2881 gtgctggcga ccaacgtcgc cgagacctcg ctcaccatcg acggcgtctc ggccgtggtg
     2941 gactccgggc tggtgcggcg gctgcgcttc gacccggcga ccggcctcga ccgtctggag
     3001 ctgggcaagg tgagccgcgc ctccgccgac cagcgggccg gacgcgccgg gcgccagggg
     3061 ccggggcact gcctgcgcct ctggggggcc tgggagcacg gggcccttcc cgagcgcgag
     3121 gctcccgaga tcggccgcgt cgatctggcg gccccggccc tccagctcct ctcctggggg
     3181 gagaccgacc tcgccgcctt cggctggttc gagccgccgg accccgccgc ccttgccaac
     3241 gccctccgtc tcctgcgcca cctcggcgcc acggacgatc acggcgtcac cgagatgggc
     3301 cggaccctcg cgcgcctgcc ggtccatccc cg
//
LOCUS       DASPJI010000732         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_560027, whole genome shotgun sequence.
ACCESSION   DASPJI010000732 DASPJI010000000
VERSION     DASPJI010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560027"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2150)
                     /locus_tag="VEL74_20495"
     CDS             complement(<1..2150)
                     /locus_tag="VEL74_20495"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG64974.1"
                     /translation="MPDPLGGEAGARLYRTGDRARWRRGGELEFLGRLDHQVKVRGFR
                     IELGEIESALSSAAGVREAVVLARTEGTGGPRLVAYVVPAAGETPAATELRELLQQRL
                     PDYMVPAVFVTLESLPRTPNGKVDRRALPAAAEARQGLAGYVAPRTPVEELLADIWSE
                     VLSVERLGVNDNFFELGGHSLLATRVMSRLRGAFQIELPLRELFEEPTVAGLAGRVER
                     AMRAGAGLLAPPLVPVARAGELPLSFAQERLWFLDQLAPGSSAYNIPVALRVVGDLDV
                     GVLRHSLEQIVRRHEALRTVFVAQDGTPVQVIEPPSRLALPVVDLSGLEEDRRERLGL
                     ELVAREAGRAFDLAQGPLLRALVVRLAEASHLALVTMHHIVSDGWSMGVLVREVSALY
                     QSYTGRRPSPLPELLVQYADYAVWQRSWLQGEVLAREIDYWRRQLAGAPALLEIPTDR
                     PRPSVQSSRGAVRGFALPAPLSSGLQDLSRRQGATLFITLLAAFQTLLARYTGQPDVV
                     VGTPIAGRNHLELENLIGFFVNTLVLRTDLSGEPGFVDLLGRVRETVLSAHAHQEVPF
                     EKLVEEVAPERSLAYSPLFQVLFALQNVPQEELSIRDLHLRPLDSPATTAKFDLTIAL
                     GEVGGELSGSVEYNTDLFDEVTIERLAGHFERLLSAVVEAPSSPAGDRALLSTAELSQ
                     LTEWSRGTGDSQRVGTCLGELFEAQVDRTPEAVAL"
BASE COUNT          373 a          876 c          650 g          278 t
ORIGIN      
        1 agcgccaccg cctcaggcgt ccggtccacc tgcgcctcga acagctctcc caggcaggtc
       61 ccgaccctct gagaatcgcc ggtcccgcgg ctccactcgg tgagttgtga caactcggct
      121 gtgctcagca gcgcccggtc ccccgccggc gacgacggcg cctccacaac cgccgacagc
      181 aaccgttcga aatgcccggc cagacgttcg atcgtcacct cgtcgaatag atccgtgttg
      241 tactccaccg acccggacag ctccccgccc acctctccca acgcaatcgt caagtcgaac
      301 ttcgccgtcg tcgccggcga gtccagcggg cgcaggtgca gatcccggat cgatagctct
      361 tcctgcggca cgttctgcag cgcgaacaac acctggaaca gcggcgagta cgccaggctc
      421 ctctccggtg cgacctcttc gaccagtttt tcgaacggca cctcctggtg cgcgtgcgcc
      481 gacagcaccg tctcgcgcac ccggcccagc agatccacga agcccggctc tcccgacagg
      541 tccgtgcgca agaccagagt gttgacgaag aaaccgatca gattttcgag ctccagatga
      601 ttgcggccgg cgatcggcgt gcccaccacc acatccggct gcccggtgta gcgcgccaga
      661 agcgtctgga acgcggccag cagcgttatg aacagcgtcg cgccctgccg ccgcgacaga
      721 tcctgcaagc ccgatgagag cggcgccgga agagcgaagc cccgcaccgc gccgcgcgag
      781 ctctgcacgg acggacgcgg acgatctgtg ggaatctcca ggagtgccgg agcacccgcc
      841 agttgccggc gccagtagtc gatctcccga gccaggacct cgccctgcaa ccagctccgc
      901 tgccacaccg cataatcggc gtactgcacc agcagctccg gcaggggcga cggacggcgt
      961 ccggtatagg actgatacag cgccgagacc tcgcgcacca gaacacccat cgaccagccg
     1021 tcgctgacga tgtgatgcat cgtcaccagc gcgaggtggc tcgcctcggc cagacgcacc
     1081 accagggctc gcagcagcgg accctgcgcc agatcgaacg cacgtcccgc ctcccgggcc
     1141 accagctcca ggcccaaccg ctcgcgacgg tcctcctcca ggcccgacag gtccactacc
     1201 ggcagcgcca gcctggaggg gggctcgatc acctgcaccg gcgtgccgtc ctgcgcgacg
     1261 aataccgtgc gcaacgcttc gtgacgtcgc acaatctgct ccagactgtg ccgcagcacg
     1321 ccgacgtcca gatcgcccac cacccgcaag gccacgggga tgttgtaggc cgagctcccc
     1381 ggcgcgagct ggtcgaggaa ccagaggcgc tcctgcgcga acgacagcgg cagctcgcct
     1441 gcccgtgcca ccggcacgag gggcggcgcc agcaacccgg cgcccgcccg catcgccctc
     1501 tccacccggc cagccagacc cgccaccgtc ggctcctcga acagctcgcg caaggggagc
     1561 tcgatctgga acgcgccgcg cagccgcgac atcacccgcg tcgccagcag cgagtgaccg
     1621 cccagctcga agaagttgtc gttcactccc agccgctcaa cactcagcac ctcggaccag
     1681 atgtcggcca gaagctcctc gaccggcgtg cgcggcgcca cgtagcccgc caagccctga
     1741 cgcgcctccg ccgccgccgg cagcgcccgg cggtccacct tgccgttcgg cgtccgcggc
     1801 aggctctcga gggtcacgaa gaccgccggc accatgtagt ccggcagccg ctgctgcagg
     1861 agctcccgca gctcggtggc cgccggcgtc tcgcccgccg ccggcacgac ataggccacc
     1921 agacgcggtc cgccggtccc ctccgtccgc gccagcacca ccgcctcgcg caccccggcg
     1981 gccgacgaca gagccgactc gatctcaccc agctcgatgc ggaagcctcg caccttgacc
     2041 tgatggtcga ggcggcccag gaactccagc tcgccgccgc gccgccagcg cgcccggtct
     2101 ccggtccggt acagccgcgc tccggcctcg ccccccagcg gatccggcac gaaacgctcc
     2161 gccgtcaggt ccggccg
//
LOCUS       DASPJI010000733         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_614452, whole genome shotgun sequence.
ACCESSION   DASPJI010000733 DASPJI010000000
VERSION     DASPJI010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614452"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VEL74_20500"
     CDS             <1..398
                     /locus_tag="VEL74_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64975.1"
                     /translation="APAADLPTTSATAVSVQASVTWLDDGTANGAWIVNPDKITVSAR
                     IELLVYTMSDKSTPGVRFLSSTPLVWISPPQPDNFDAQVSEDGRQLFIADRNQTTGLP
                     EAFSFRLLAVYDDITRISPDPMIINLDPS"
     gene            complement(697..>2163)
                     /locus_tag="VEL74_20505"
     CDS             complement(697..>2163)
                     /locus_tag="VEL74_20505"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG64976.1"
                     /translation="APLPPEQVDPVPGTPLELARATAAGEVVTSRLDCAGGTCQVALS
                     RLSGRDGSVLWTQGFAAPVDRPYLLAEAVEGYLRRGYPDHRVRHGASTLQVRPEDYAE
                     YLGLRQAFEKEQQEELPAAALESLAGIRRRSPRFLEAFVFEAEVLQHRFQVRRDPEDL
                     ERATRLLAAARELAPADPRPLFTQFEVDLRRQRLDRAEEVLRDLDRLQPGDPGILVLR
                     ARLLEARGRGEEALAAIREAVRRSPSWRHLHWQARLEQRHGDTAAARRSLEELLRRFP
                     DSALGQTTLANLELLTGSPERAAELYERLVRDASQTHLLSNLGLAYMLLGRYAEAEES
                     FRQALALSPRSPESALNLADVHFLQGKWSEAVKLYKEVIRLVDEDPAADQAQLQATRA
                     QALAHLGRGSEAVAAVQQALRQSPDDPQIAYAVSLVYAVVGDDTSALLNAKRALEDGV
                     EPRWFTLPWFAPLRAAPELQAVGPWPSSPGNSSPSLRR"
BASE COUNT          303 a          801 c          776 g          283 t
ORIGIN      
        1 ccgcccccgc cgccgacctc cccacgacct ccgccaccgc cgtgtccgtc caggcgagcg
       61 tcacctggct tgacgacgga acggcgaacg gcgcgtggat cgtcaatccc gacaagatca
      121 cggtcagcgc caggatcgag ctcctcgtct atacgatgag cgacaagagc acgccgggcg
      181 tccggttcct gtcctccacg ccgctggtgt ggatctcgcc gccgcagccc gacaattttg
      241 acgcccaggt atccgaagac ggccggcagc tcttcatcgc cgaccgcaac cagaccaccg
      301 gcctccccga ggccttctcc ttccggctgc tggcggtcta tgacgacatc acccggatct
      361 cgcccgaccc gatgatcatc aacctggacc cgagctaggg ccctcacccc cggccccctc
      421 tcccgcctcc ccccctcccc ggaagagggg gaggcggaca tcctaggcgg gatcaccgcg
      481 aaaacggcag ccttccagcc gaagaagctg ccctccccca tggatggaca gccgtccttc
      541 cggggacggc cttcctaccc ggggcaggga cctcatcccc ggcgccacgg acctcatcgc
      601 tccgggtacg gccttcctac ccgggggatg gcctttccac ccacgcgcac ggccctcatg
      661 cccgcgggga tggcccccgc ggcgcgggga cggagctcat cgccggaggg atggcgatga
      721 attgccgggg gatgacggcc acggccccac cgcctgcagc tccggcgcgg cgcgtagcgg
      781 ggcgaaccag gggagggtga accagcgcgg ctcgacgccg tcttccaggg ctcgcttggc
      841 gttgagcagg gccgaggtgt cgtcccccac caccgcgtag accagggaga cggcgtaggc
      901 gatctggggg tcgtccggcg actggcgcag cgcctgctgg acggccgcca cggcctcgga
      961 gccgcgcccg agatgggcca gcgcctgggc gcgagtggcc tggagctgcg cctggtccgc
     1021 agcagggtct tcgtccacca ggcggattac ctccttatag agttttacgg cctcgctcca
     1081 cttgccttgg aggaaatgga cgtcggcgag gttgagggcg ctctccgggc tgcgggggct
     1141 cagggcgagg gcctgccgga agctctcctc cgcctccgcg tagcgcccca ggagcatgta
     1201 ggcgagcccc aggttggaca gcaggtgcgt ctgcgacgcg tcgcgcacca gccgctcgta
     1261 gagctcggcg gcgcgttccg ggctgccggt gagcagctcc aggttcgcca gggtggtctg
     1321 gccgagagcg gagtcgggga agcgccgcag cagctcttcc agggagcggc gggccgccgc
     1381 ggtgtcgccg tgccgctgct cgaggcgcgc ctgccaatgg aggtggcgcc aggaggggct
     1441 ccgccgcacc gcctcgcgga tggcggcgag cgcctcctcc ccccggccgc gggcctccag
     1501 cagacgggcg cgcaggacga ggatccccgg gtcccccggc tgcagccggt cgaggtcgcg
     1561 cagcacctcc tccgctcggt cgagccgctg gcgcctgaga tcgacctcga actgggtgaa
     1621 gagaggacgc ggatcggcgg gcgccaactc acgggcggcg gccagcagcc gggtggcccg
     1681 ctcgaggtcc tccgggtcgc gccgcacctg gaaacggtgc tggaggacct cggcctcgaa
     1741 gacgaacgct tcgaggaagc gcggggagcg gcggcggatc cccgccaggc tctccagggc
     1801 cgccgccggc agctcctcct gctgttcctt ctcgaacgcc tggcgcaggc ccaggtactc
     1861 ggcgtagtcc tccggccgca cctggagcgt cgaggcgccg tggcggaccc ggtgatcggg
     1921 gtagccgcgc cgcaggtagc cctcgacggc ctcggccagc aggtaggggc ggtccaccgg
     1981 cgcggcgaag ccctgggtcc agaggacgga gccgtcgcgg cccgacagcc gggagagggc
     2041 gacctggcag gtgccgccgg cgcagtcgag ccgcgacgtg accacctcgc cggccgccgt
     2101 cgcccgcgcc agctccagcg gggtgccggg cacggggtcc acctgctcgg gggggagcgg
     2161 cgc
//
LOCUS       DASPJI010000734        14761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_217155, whole genome shotgun sequence.
ACCESSION   DASPJI010000734 DASPJI010000000
VERSION     DASPJI010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14761)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14761
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217155"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="VEL74_20510"
     CDS             <1..1119
                     /locus_tag="VEL74_20510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64977.1"
                     /translation="TLAKLVRDGRLPAPRRTIRFVWPPEIEGTIALLNARPDLAGRAR
                     AVIHMDMVGGDAEITKAVFRVTRSPKSLPTFVNDVAEAFGRFANAQSDLYASTRSAPY
                     PLVDPEGGKRALQADFVDFTEGSDHQVWTEGSWRVPAIYLNDWPDRYIHTHHDSIANI
                     DATKLLRAAFLGAAGAYYLADLGADDVPALWQVVRRHALERGADLLARQERLRAAGQA
                     GEIANLSRFHLAYEQAVLDSIAPFAEVPAAVRQDAGRLLASLRTLAGDPPAPGRASGT
                     VYRRNAELKGPMEGFGYEYLEDKMTGLGITGPARPALLGYEGLWGASYPYEALNLVDG
                     RRTVGEIRDALAAIYGPVPLEQVSGFLEVLERAGVLRR"
     gene            1308..2027
                     /locus_tag="VEL74_20515"
     CDS             1308..2027
                     /locus_tag="VEL74_20515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64978.1"
                     /translation="MSNVFRPKSVFVAASAMLLAGAVAFAGVQTDKQLARVKKDRNGN
                     VSFKQVAGYTGYETDNEYHAKIKELTDPQAIYELKMKHHQLNIEHELNGGVLHGSIER
                     GGSLITIDIVGTGTAAGFHHSLTFPAKFEVHSEPHDPSVEIDTFRTNMYRIEGAAGGD
                     SLFSSLRLVGGTANGFASPGQMTLIDKGDSVLVDSFFNIGYRIEFTGADGGPLAGVQD
                     SFEGTVTMRAHGTEQAVTGGK"
     gene            2165..2821
                     /locus_tag="VEL74_20520"
     CDS             2165..2821
                     /locus_tag="VEL74_20520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64979.1"
                     /translation="MPSYTLQLMISAQDLTTLRQAGESIVIARAFSGSSPNVAWVSFA
                     PFSSNTVTWDDAYGLFASPAQSSGTIQILSQTSGTAASGMYYNFADASFSGPFSGASA
                     PGAGSFRVFNQMPSSQYTALGFGMLQSATVNGSALPARPANLDIVPAMQFSTFTPLNT
                     VYVWLQSGVSSGDIVTLPPLSMGAALAVSSRATKVQFSGTSSLSYVYESSVGAFVPAN
                     "
     gene            2999..3646
                     /locus_tag="VEL74_20525"
     CDS             2999..3646
                     /locus_tag="VEL74_20525"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HYG64980.1"
                     /translation="MSGDDLKEIFLGSGRAGRVQVERHQDIYICGDTERLIYFVESGS
                     VRVSRPSAAGRDCLLRIELAGGIFGELALLEAHERLETATAREKGAVWRMRGSDFWRE
                     LRKRDLLEPFVSHLILRMADQDRRIAHLITEPSLRRLGKMLLMLAHRLGTRTREGFRI
                     GTRISHEELADLVGTTRPRVSTFLCDLRRRNLIEVSDNRHLVIHEESLRRFLGDF"
     gene            3633..4172
                     /locus_tag="VEL74_20530"
     CDS             3633..4172
                     /locus_tag="VEL74_20530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HYG64981.1"
                     /translation="MATSRGAAPEEGSVERLLPQVYDELLRLARRRLARYGPGESITP
                     AELVHEAYLRLAGGTRRRFEGRPHFFFAMSRTMRDLIVESARRRATSRRGGGRPAVDL
                     YEADARGVERKDFVDLHRALLRLAEHDRECARTVVLSYFVGLTHPEIAEATGVSRATV
                     ERRWACARAWLRRELADRA"
     gene            4267..4479
                     /locus_tag="VEL74_20535"
     CDS             4267..4479
                     /locus_tag="VEL74_20535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64982.1"
                     /translation="MATIVVTKGDLNWGKGEGSVDSKAGTASITSWVDQAAMTVGTTY
                     GLKTGGKSYTGKCQSSTLPINFTDVE"
     gene            4514..5257
                     /locus_tag="VEL74_20540"
     CDS             4514..5257
                     /locus_tag="VEL74_20540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64983.1"
                     /translation="MPTFTLNTTFDQSALSMLYATGSNVVVAKPSSSGGTPNVAWVVY
                     RPMPDNVMTWEEQYGIYASTSSIQNGAKLKQMSATPFPAGAGKVYTLQSDSNFSSPQS
                     GGTANSYTAINQYDNLAPNGPGWLTFGLFQNATVNQSALSGNAVSAAGVPFQSTAVMT
                     PYTTVYIWTQSQVVSNTVVTLVTSTMTSVKFGGSVTEISLQYDQTTGGFVNVGGALKM
                     DARSNLLVASGGEELPSEASLAYHIPLLG"
     gene            complement(5336..6313)
                     /gene="cysK"
                     /locus_tag="VEL74_20545"
     CDS             complement(5336..6313)
                     /gene="cysK"
                     /locus_tag="VEL74_20545"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HYG64984.1"
                     /translation="MGRIFDSIIDTIGRTPLVRAPNVSKSLGLEADVVFKLESFNPMS
                     SVKDRIGYSMVLDAIEKGVIKEGTTVIEPTSGNTGIGLAFVCAAKRIPLVLTMPDTMS
                     MERRQILKALGAKLELTEGAKGMKGAMAKAEEIQKETPGSIIIGQFSNPANPEIHRRT
                     TAVEIWDDTDGKIDILVVGVGTGGTVTGCSDVLKKKKPALQSIVVEPTDSPVLTQKRA
                     GQELVPGPHKIQGIGAGFIPEIMDLDLVDDVFRVTNDQALEMGRRLCREDGMMVGISS
                     GAVAHAAFEIARRPENKGKLIVAVLASHGERYLSTLLYQFPEDEQPTSP"
     gene            complement(6342..7322)
                     /gene="epsC"
                     /locus_tag="VEL74_20550"
     CDS             complement(6342..7322)
                     /gene="epsC"
                     /locus_tag="VEL74_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HYG64985.1"
                     /translation="MKRHPFSNRLQGITREVVATYGAAGDPASHLHPDRRLPSRTAVA
                     RILDNLCAVLYPGYYGVQHLTPENVEYHVGALLDDIAADLYDQVLLAYPFAVEEVPPE
                     GQAAPGELEPAQTARLADHAVERFLGRIPELRRLLILDVKASMEGDPAARSFAEIIFA
                     YPGFEAITVQRIAHALWDIGVPLLPRIMTELAHSKTGIDIHPGAQIGESFFIDHGTGV
                     VIGETAVLGRNVKVYQGVTLGALSFPKDAGGRLLRGAKRHPTLEDNVVVYAGATILGG
                     DTIVGEGAVIGGNTWVIESVPPGERVLYTRTSRAKQKQILERAYGFGEGI"
     gene            7446..7805
                     /locus_tag="VEL74_20555"
     CDS             7446..7805
                     /locus_tag="VEL74_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYG64986.1"
                     /translation="MDKVNLAEKLSRFNDHWNPRIVGELNGQHVKLAKLAGPFVWHHH
                     EHEDELFLVVKGRFRMEFRDRSVDLSEGEFLIVPRGVEHRPVAEEEVHVLLFEPAATL
                     NTGNVRSEVTRDELERI"
     gene            7786..8412
                     /locus_tag="VEL74_20560"
     CDS             7786..8412
                     /locus_tag="VEL74_20560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64987.1"
                     /translation="MSWSGSEQGESPIGARGRILPAQLAWYVTGRFYVDGDGAILDAG
                     YFLHLQDVTGTLFLGAPGEGTACFTFLAEPFNADRLTNGDLSLAVDAVGEFSLFLNRD
                     PGGPTFDDPRSFGAGERIATFRRTSIVVGSTVGGVDGAPAVVSVNLFSAALVESKEFE
                     HGGRTLDLGRLLPNGITQWGTASTKSISPLPAGYTAAVPFVGSAVAIG"
     gene            complement(8418..9044)
                     /locus_tag="VEL74_20565"
     CDS             complement(8418..9044)
                     /locus_tag="VEL74_20565"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HYG64988.1"
                     /translation="MKELPQPYPPMLLSEVKQPFHKTGWFYEEKYDGYRAMAYKDGSR
                     VQLISRNMKDLTKQFEEVAEAVARLKAPTLILDGEIAVFDENLVSHLGYLRSSKEEKL
                     LTPPVFVAFDCTYARGKDLLREPLRRRRTVLEKELRDAEGPILVARRLAENGLAAWQE
                     VKARGWEGLIAKDPASVYEPGVRTRSWIKVKHKVRVGWPGEGTEYTRG"
     gene            complement(9511..10032)
                     /locus_tag="VEL74_20570"
     CDS             complement(9511..10032)
                     /locus_tag="VEL74_20570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64989.1"
                     /translation="MHDPLQGSTPLPNAFSVEFLRQDLERSEPAEVDDPVGEGPWVVL
                     PSPAGDWPEWEAVHGLWPQGGQPDRGDPSPFVASGRSLALCSALARPIAGRDPLYVLG
                     QERWRDGFPLLRQGRPEGWMATFFPEWALITSALVSLGQRPYDVAVFLEALGPSCRER
                     AGYFLHERLAGSR"
     gene            11033..12328
                     /locus_tag="VEL74_20575"
     CDS             11033..12328
                     /locus_tag="VEL74_20575"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HYG64990.1"
                     /translation="MTKAEVAELKELACLRDEPEALFSDRRGRERKGLSMFLRLRPQP
                     FGAVINTLRRGDSLLINGGPEPRRAAIDVCAQPRPDMGPIPCATDCVDDFFPAIRTGR
                     ELARYFESETPGLAHRLALSYLLRDANYSPPYQAFIWAALDVAIYSALGAAWYYKWIA
                     DSNCELREPCRPMSRYRRERTSRRPRPYEVDYEVDVLFNRAVNCTNSGDGERRLLPDP
                     SPGTPRHPSYPSGHSTYAGAASEILSYFFPDYSGELTKLADNTGMGRLWAGIHYRSDH
                     IEGMKLGRCVARLIIRQIEGSCIVRPDPCDSAAASVADCKKGPPSREHLCRCAQELCE
                     CCARRGARHAAAGLQEESVAPGRQTQEGEGGEEGEGGGAGSSAAVREQARGPQEGAGV
                     AGSSGEAREQAQGPQEGAGTAGSSEGAREQAKGPQEGAQ"
     gene            12406..13029
                     /locus_tag="VEL74_20580"
     CDS             12406..13029
                     /locus_tag="VEL74_20580"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYG64991.1"
                     /translation="MTTAYIDHVLVGVPDLEAGIGAMEGLMGVRPVLGGEHPHWGTHN
                     ALLSLGDGIYFELIAVRPGAEAPKFEHLRSLSAPTPIGWAVRVDDPREVSSLLRGAGL
                     APTDTKPGSRQTPAGAVLRWETFDLDPAPAGSPFFIHWLPETAHPSKTSPSGCSLSRF
                     EIASPDAAELVRLRDALGLRLDIREAETPRMTLTLRCPKGTVTLPAT"
     gene            13175..>14761
                     /locus_tag="VEL74_20585"
     CDS             13175..>14761
                     /locus_tag="VEL74_20585"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG64992.1"
                     /translation="MPHASHRAPDDPTLPIRLAVYLLSGLIPAAERDEVAGDLRDEYA
                     HRLGAQGRAAAAVWLWRQVLGSVPALLRRSWWRGWTGFEPAANRLLPGGTWMESWIMD
                     LRYAVRRLRSRPTYVLLAVLTLALGVGGTAAILSIARGLLVDPLPYEKEEEVGVFWNP
                     HDWSEAEFLYLRPEFPGFSQVAAYRPEDVTLEPREAPARVLTGISATAELFQVLGTRP
                     ALGRVFQPGDDRQGGDPVVVISHGLWQEMGGDASVIGRRVQLDGIPRTVVGVMPRGFW
                     FPDPTVRVWLAEQLEPTNGSGMYALVGRVAPGQRIEGITPTLARITMALGERFQYPEQ
                     WDKTKNAAVTPIREYLVGSLRPALLATLAAMAVILLIACANVAALMLDQVNRRAGELA
                     VRSSLGADRGRITQQLVIEALVIGLLAGGAGALLAAGGFRVLAGALPLGAWAESAELD
                     WTLFGAAIGLALLAALGVALIPAMSLWRGDLRDALTRVRTATAGGQGGRVESGLVVAE
                     VALAVLLAAGAALMIRSVGNL"
BASE COUNT         2347 a         4950 c         5040 g         2424 t
ORIGIN      
        1 acgctcgcca agctggtgcg ggacggcagg ctccccgcgc cgcggcgcac catccgcttc
       61 gtctggccgc cggagatcga ggggacgatc gcgctgctca acgcccgccc ggacctcgcc
      121 ggccgcgccc gcgccgtcat ccacatggac atggtcggcg gcgacgccga gatcaccaag
      181 gcggtcttcc gggtcacccg ctcgccgaag agcctgccca ccttcgtcaa cgacgtcgcc
      241 gaggccttcg ggcgcttcgc caacgcgcag tcggacctct acgcctccac ccgctccgca
      301 ccctatccgc tggtcgatcc ggaaggaggc aagcgcgccc tccaggccga tttcgtcgac
      361 ttcaccgaag ggagcgatca ccaggtctgg accgagggct cgtggcgggt gccggccatt
      421 tatctgaacg attggcccga ccgctacatc cacacgcatc acgattcgat cgccaacatc
      481 gacgccacca agctgctgcg cgccgccttc ctcggcgccg ccggcgccta ttacctggcg
      541 gatctgggcg cggacgacgt gccggccctc tggcaggtgg tccgccgcca cgccctggag
      601 cgcggcgcgg acctgctggc gcggcaggag cggctgcgcg ccgcggggca ggccggggag
      661 atcgccaacc tgtcacgttt tcatctcgcc tacgagcagg cggtgctcga ctccatcgcg
      721 ccgttcgccg aggtgccggc cgcggtgcgc caggacgccg gacggctcct cgcctccttg
      781 cgcaccctgg ccggagatcc gcccgcgccg ggccgggcgt ccgggaccgt ctaccggcgc
      841 aacgcggagc tcaaggggcc gatggaaggc ttcggctacg aatatctgga agacaagatg
      901 accgggctcg ggatcaccgg gccggcccgc ccggcgctgc tcggctacga aggcctgtgg
      961 ggcgccagct acccctatga agccctgaac ctggtggacg gacggcggac cgtcggcgag
     1021 atccgcgacg ccctggcggc gatctacggc ccggttcctc tggagcaggt gtccggtttc
     1081 ctcgaggtgt tggagagggc cggcgtgctg cgtcgttgag cgaccccgga gtttctgaca
     1141 ttcacctctc ctgtcccgcg ccggctgcct gacgcaccgc ttcaggcagg ggaaggaaga
     1201 gcgggcccct ggctccctcc tgggagagcc ttgccgtggc gcgtgcccac ggctcacgtc
     1261 agacgtgtct ccggcccgcg gagacataga ggttcgggag gtttttcatg tcgaatgtgt
     1321 ttcgtcccaa gagcgtcttt gttgcggcct cggccatgct ccttgccgga gccgtggcgt
     1381 tcgccggtgt gcagaccgac aagcaactcg cgagggtcaa gaaggaccgc aacggcaacg
     1441 tgagcttcaa gcaggtcgcc ggctacaccg gctacgagac cgacaacgag tatcacgcca
     1501 agatcaagga gctcaccgat ccgcaggcga tctatgagct caagatgaag catcaccagc
     1561 tgaatatcga gcacgagctc aacggcgggg tcctccatgg ttcgatcgag cggggcgggt
     1621 ccctgatcac gatcgacatc gtcgggaccg ggaccgcggc cggcttccat cactcgctca
     1681 ccttcccggc gaagttcgag gtccattccg agccccacga tccgagcgtc gagatcgaca
     1741 ccttccgtac gaacatgtac cggatcgagg gcgcggccgg cggtgacagc ctgttctcgt
     1801 cgctgcgcct ggtgggcggc accgccaacg gctttgcgag cccgggccag atgaccctga
     1861 tcgacaaggg cgactccgtc ctcgtcgaca gcttcttcaa catcggctac cgcatcgagt
     1921 tcacgggcgc tgacggcggc ccgctggccg gagtccagga ttccttcgag ggcacggtga
     1981 cgatgcgggc tcacggcacc gagcaggccg tcaccggcgg caagtgatca ctcccgcgag
     2041 gagctgattc atgaaacaaa agggcggagg ccgcgcggct ctccgccctt tcgtctccag
     2101 gcccacggtt gacaggagag aggggcggct cccagaatac ggcccactca tctcaggagg
     2161 tcctgtgccg tcctacaccc tgcaactcat gatcagtgcc caggatctga ccaccctccg
     2221 ccaggcgggc gagagcatcg tcatcgccag agcgttcagc ggctcctctc ccaacgtcgc
     2281 ctgggtgagc ttcgccccct tctcgtcgaa caccgtcacg tgggacgacg cttacgggct
     2341 cttcgcctcg ccggcgcaat cctcgggcac catccagatc ctgagccaga cctcgggtac
     2401 ggccgcctcc ggcatgtact acaacttcgc ggacgccagc ttctcgggcc cgttctcggg
     2461 agccagcgca cccggggctg gatctttccg ggtcttcaac cagatgccgt cctcgcaata
     2521 caccgctctc ggcttcggaa tgctccagag cgccacggtg aacggctccg cccttcccgc
     2581 caggcccgcc aacctggata tcgtcccggc catgcagttc tccaccttca cgccactgaa
     2641 taccgtgtat gtctggctcc agtcgggggt gagcagcggc gacatcgtca cgctcccccc
     2701 attgtctatg ggggccgccc tggccgtctc cagccgtgcc accaaggtcc agttctcggg
     2761 cacctcctcg ctttcctacg tgtacgaatc gtccgtcggc gcgttcgtcc ccgctaactg
     2821 accggtctcg agaccgcatc tccgctccct ccgcccgtcc ggtaccgcgg tgctccgccg
     2881 cgtgcgccgt tgttccattt tgaactgaaa cacgccgcgg actcgcggat actacgctct
     2941 cccgaagacg atcccgcgaa agtgatctct catgaccagt tctctgctcc ggagcgtgat
     3001 gtcgggtgac gacctgaaag agatctttct cgggagcggg agggctggaa gggtgcaagt
     3061 cgaaagacat caagatatct acatctgtgg tgatacggaa cggttgatct atttcgtcga
     3121 gagcggctcg gtcagggtca gcaggccttc ggcggctgga agggattgcc tgctgaggat
     3181 cgagctggca ggggggatct tcggcgagct cgcgctcctg gaggcgcacg agcggctcga
     3241 gacagccacc gcgcgagaga agggggccgt ctggaggatg cgcggcagtg atttctggcg
     3301 cgagctgcgg aaacgtgacc tcctcgagcc gttcgtcagc catctgatcc ttcgcatggc
     3361 ggatcaggac cggcgcattg ctcatctcat cacggaaccg agcctgcggc ggctcggcaa
     3421 gatgctgctc atgctggcgc acaggctcgg tacccgcacg cgagagggct tcaggatcgg
     3481 tacgcggatc tcgcacgagg agctggcgga tctcgtcggc accacgcggc cccgggtgag
     3541 cacgttcctc tgtgatctca ggcgccgaaa cctgatcgaa gtgagcgaca accgccacct
     3601 cgtgatccat gaggagagcc tcaggagatt ccttggcgac ttctagggga gcggcgcccg
     3661 aggagggctc ggtggagcgc ctcctgccgc aggtctatga cgagctgctc cgtctggcca
     3721 ggcgcaggtt ggcgcggtat gggccgggtg aatcgatcac tccggccgag ctggttcacg
     3781 aggcctacct gcggctggca ggaggcacgc gccgccgctt cgaggggcgg cctcacttct
     3841 tcttcgccat gagccggacg atgcgggatc tgatagtgga gagcgcccgg cgccgagcga
     3901 cctcgaggcg cggcgggggc cgtccggccg tggacctgta cgaggcggat gcccgcggtg
     3961 tggagcgcaa ggacttcgtg gacctccacc gcgcgctgct gaggctggcc gagcacgacc
     4021 gcgagtgcgc gcggaccgtc gtattgagct acttcgtggg cctcacccat cccgagatcg
     4081 cggaggccac gggtgtatcg cgcgccaccg tcgagaggcg ctgggcgtgc gctcgcgcct
     4141 ggctgcgccg cgagctcgcg gatcgagcat gaggggattt tggaatggat atcggcttga
     4201 caaagcagaa ggtgtccgga caccctcacg aaccccgagg ctgactttca cgaaaaggag
     4261 aagaagatgg caaccatcgt cgtgaccaag ggtgacctga actggggcaa gggggagggg
     4321 agcgtggatt ccaaggccgg gaccgcgagc atcaccagct gggtcgacca ggccgccatg
     4381 acggtcggca cgacctacgg tctcaagacc ggaggaaagt cgtataccgg gaagtgtcag
     4441 agcagcacgc ttcccatcaa cttcacggac gtcgagtgaa ccttccagag cacaggttga
     4501 gaggagaatc gagatgccaa cgttcacgct gaacaccacc ttcgatcaga gcgcgttgtc
     4561 gatgctctac gcgacgggat ccaacgtcgt cgtggccaag ccgagcagta gcgggggcac
     4621 ccccaacgtc gcctgggtgg tctatcgtcc gatgccggac aacgtcatga cctgggaaga
     4681 gcagtatggg atctatgcgt cgacttcgag cattcagaac ggggcaaagc tcaagcagat
     4741 gtccgcgacg ccgttcccgg ccggcgcggg gaaggtctac acgctgcagt cggacagcaa
     4801 cttcagcagc ccgcagtcgg ggggcacggc caacagctac accgcgatca atcagtacga
     4861 caacctggcg ccgaatgggc cgggttggct cacgttcggc ctgttccaga acgcgaccgt
     4921 caatcagtcc gctctgagcg ggaacgcggt ctccgcggcc ggcgtgccct tccagagcac
     4981 ggccgtgatg accccctata cgaccgtgta catctggacg cagtcccagg tggtgagcaa
     5041 caccgtggtg accctcgtga cctccacgat gacgtcggtg aagttcggcg ggtcggtgac
     5101 ggagatctcg ctgcagtacg accagaccac aggtggcttc gtcaatgttg gtggggcctt
     5161 gaagatggat gcgcgctcga acctgctggt cgccagcggc ggtgaggagc tgccgtcgga
     5221 ggcctccctg gcctatcaca tcccccttct gggctagcct cggcgggcgc cggagcgaaa
     5281 agggcggagg gcgccttgcg gccctccgcc cttcttgttt cgggagaaac ccttcctacg
     5341 ggctggtggg ctgctcgtcc tcggggaact gatagagcag cgtcgagagg tagcgctcgc
     5401 cgtggctggc gaggaccgcg acgatcagct tccccttgtt ctcggggcgc ctggcgatct
     5461 cgaaggcggc gtgggccacg gcgccggagg agatccccac catcatgccg tcctcgcggc
     5521 agaggcgccg ccccatctcc agcgcctgat cattggtgac gcggaacacg tcgtccacca
     5581 ggtcgaggtc catgatctcc ggaatgaagc cggcgccgat cccctggatc ttgtgggggc
     5641 cgggcaccag ctcctggccg gcgcgcttct gtgtcagcac cggcgagtcg gtgggctcga
     5701 cgacgatcga ctgcagcgcc ggcttcttct tcttgagcac gtcgctgcag ccggtgaccg
     5761 tgccgccggt gccgacgccg acgacgagga tgtcgatctt gccgtcggtg tcgtcccaga
     5821 tctcgaccgc cgtggtgcgg cggtggatct ccgggttcgc ggggttggag aactgcccga
     5881 tgatgatcga acccggggtc tccttctgga tctcctcggc cttggccatc gcccccttca
     5941 tccccttggc gccctcggtg agctccagct tcgcgcccag ggccttgagg atctgccggc
     6001 gctccatcga catggtgtcc ggcatggtca gcaccagagg gatccgcttc gccgcgcaga
     6061 cgaaggcgag gccgatgccg gtgttgcccg aggtgggctc gatgaccgtc gtcccctcct
     6121 tgatcacgcc cttctcgatg gcgtcgagga ccatcgagta gccgatccgg tccttgacgc
     6181 tggacatcgg gttgaacgat tcgagcttga acaccacgtc cgcctcgagg cccagagact
     6241 tgctgacgtt gggtgcccgc accagcggtg tgcggccgat ggtatcgatg atgctgtcga
     6301 aaatccgccc catggcgtcc tcccctgaga taggtcgact gctaaattcc ttccccgaac
     6361 ccgtaggcgc gctcgaggat ctgcttctgc ttggcacggc tcgtgcgggt gtagagcacc
     6421 cgctcgcccg gtggaaccga ttcgatcacc catgtattgc cgccgatgac cgccccttca
     6481 cccacaatgg tgtcgccacc gaggatcgtc gcgccggcgt agaccacgac gttgtcctcg
     6541 agggtggggt ggcgcttggc gccgcgcagc aggcggccgc ccgcgtcctt ggggaacgag
     6601 agggcgccga gggtgacgcc ttgatagacc ttgacgtttc tgccgagcac cgcggtctcg
     6661 ccgatgacga cgccggtgcc gtggtcgatg aagaaagact cgccgatctg cgccccgggg
     6721 tggatgtcga tcccggtctt cgagtgggcg agctcggtca tgatgcgggg cagcaggggg
     6781 acgccgatgt cccacagcgc gtgcgcgatg cgctggaccg tgatggcctc gaagccgggg
     6841 taggcgaaga tgatctcggc gaagctgcgg gccgccggat ccccctccat cgaggccttg
     6901 acgtcgagga tcaggagccg ccggagctcc gggatgcggc cgaggaagcg ctccaccgcg
     6961 tggtccgcga gccgcgcggt ctgcgcgggc tcgagctccc cgggcgccgc ctgtccctcg
     7021 ggcggcacct cctcgacggc gaaggggtag gccaggagga cctggtcgta caggtccgcg
     7081 gcgatatcgt cgagcagcgc cccgacatgg tactcgacgt tctccggcgt caggtgctgc
     7141 accccgtagt acccgggata gaggacggcg cagagattgt cgaggatgcg cgccacggcg
     7201 gtgcgcgagg ggagccggcg gtccggatgc agatgagagg cggggtcgcc ggcggcgcca
     7261 taggtcgcca cgacctcccg cgtgatgccc tgcagccggt tcgagaaggg atggcgcttc
     7321 atgacggaat cctaactcaa ctcgggcgcc gggcagtccc gccggtccgc cccacccccg
     7381 ccccgggctc gaccgggtgc cgctccggcc tctagaatgc gccgagcggc aggagaggag
     7441 gaaggatgga caaggtcaac ctggcggaga agctctcccg tttcaacgat cactggaacc
     7501 cgcggatcgt cggcgagctg aatggtcagc acgtgaagct ggcgaagctc gccggcccct
     7561 tcgtctggca ccatcacgag cacgaggacg agctgttcct ggtggtgaag gggaggttcc
     7621 ggatggaatt cagggaccgg tccgtggatc tctccgaggg ggaattcctg atcgttccac
     7681 gcggcgtcga gcaccgcccc gtggccgaag aagaggtcca cgtcctcctg ttcgagcccg
     7741 cggcaacgct caacaccggc aacgtccgga gcgaggtgac gagagatgag ctggagcgga
     7801 tctgaacagg gcgagtctcc gataggagcc agggggcgga tcctgcccgc gcagctcgcg
     7861 tggtacgtga cgggccgctt ctacgtcgac ggcgacggcg cgatcctcga cgccgggtac
     7921 ttccttcacc tccaggacgt gacggggacc ctcttcctcg gcgcgcccgg cgagggcacc
     7981 gcctgcttca ccttcctcgc cgagccgttc aacgcggacc gcctgacgaa cggcgacctg
     8041 agcctggccg tcgacgcagt cggcgagttc agcctgttcc tgaaccgcga tccgggcggc
     8101 ccgacgttcg acgatccacg gagcttcggc gcgggggagc ggatcgcgac gttccggcgg
     8161 acgagcatcg tggtcggcag cacggtggga ggcgtggacg gagcgccggc ggtcgtctcg
     8221 gtgaacctct tctccgcggc gctcgtcgag agcaaggagt tcgagcacgg cggccgcacc
     8281 ctggatctcg gccggctcct gccgaacggc atcacgcagt ggggcacggc gagcacgaaa
     8341 tcgatctcgc cgctcccggc cggctacacg gccgcagtcc ccttcgtcgg ctccgcggtc
     8401 gcaataggat agaaatttca cccccgggtg tattcggtcc cctcgcccgg ccagccgacg
     8461 cggaccttgt gcttgacctt gatccagctc cgggtccgca cccccggctc gtagacggac
     8521 gccgggtcct tcgcgatgag cccctcccag ccgcgggcct tcacctcctg ccaggccgcg
     8581 agcccgttct cggccagccg gcgggcgacg aggatcggtc cctcggcgtc ccggagctcc
     8641 ttctcgagca cggtacgccg ccggcggagc ggctcgcgca ggaggtcctt gccgcgggca
     8701 taggtgcaat cgaaggcgac gaagaccgga ggggtcagga gcttctcttc cttggacgac
     8761 cggaggtagc cgaggtgtga cacgaggttc tcgtcgaaca ccgcgatctc gccgtcgagg
     8821 atgagcgtgg gggccttgag ccgcgccacg gcttccgcga cctcctcgaa ctgcttcgtc
     8881 aaatccttca tattgcgtga gatgagctgg acacgcgacc cgtccttgta ggccatcgcg
     8941 cggtagccgt catatttctc ttcatagaac cagcccgtct tgtgaaatgg ctgtttgacc
     9001 tcggaaagca gcatcggcgg gtagggctgg ggcagctcct tcatgccgag cggcagtttg
     9061 caagaagcaa accgtaggca tttccctcac gccacggcaa atgaagttgg ctcgacttca
     9121 tttgccgtga gccgacttga tttcaagtgg gccgacttca aacgccgtgg gccgctgtca
     9181 aacgcggcgg cacgcctgaa gacgcggtgg ggcgcctcga gacgccgccg gccaacttca
     9241 aacgcggtgg gccgacttca tttgccgcga gccgacttga aaccccgccg ggcaactcca
     9301 aacgccgtga gccagcttca aatgccgtga gccgcttcaa atgccgtgag ccgacttgaa
     9361 acgcggcggg ccgacttcat ttgcggcgag ccgacttcga acgccgtgag gcgccagcaa
     9421 acgccgtggc atgactttcc cggcggacag gaaggcggag ggccgggagg gccctccgcc
     9481 tgatctcacc tagataagga agggacgcct tcagcgggag ccggcgaggc gttcgtgaag
     9541 gaagtacccg gcccgctcgc ggcaggaggg gccgagggct tcgaggaaga cggcaacgtc
     9601 gtagggccgc tggccgaggc tgacgagggc gctggtgatg agggcccact ccgggaagaa
     9661 ggtggccatc cacccctccg gacgtccctg gcgcagcagg gggaacccgt cgcgccaccg
     9721 ctcctgtccg aggacgtaga gcggatcacg gccggcgatg ggccgggcga gggccgaaca
     9781 gagggcaagc gaccggccgg acgcgacgaa cggcgacgga tcaccccggt ccggctggcc
     9841 accctggggc cagaggccgt ggaccgcctc ccactccggc cagtctccgg cgggggaggg
     9901 gaggacgacc cacgggccct cgcccacggg atcatccacc tcggcgggct cgctacgctc
     9961 gaggtcctgg cggaggaatt cgacggaaaa cgcgttgggc agcggagtgc taccctgaag
    10021 ggggtcatgc ataggagtac aagctccttt gtgtgatcaa ggctcggggt gggccggcca
    10081 gctcactccg tgccgttaag gctctctcgg cggtcggcca cgcgccggcc gcctcgtcca
    10141 gctagctgct gcgtctcttc cttgcctccg cgaccacctc ctctctcccg gtaggacgac
    10201 atcgttccgt ttgcgacacc cgtactctag cgcggggtct cgggggacgt caaggggtgt
    10261 ggcggagatt tgtgacagcc ttggggagag gtcgaagctg cgactccccc tcgcctcggg
    10321 cttgcgggca ggggacttcg gtgctcaacg atctctactg ctacttcgac tacatcggtg
    10381 actggaagtt caaggaccct ttcgcggcta tcgcgctcaa gcgagacgtg agcgattgac
    10441 catccgcctc ctcgggccgg cccgcgcctc gatcccggag cccccgatct ctgggtgaac
    10501 gaggccggag ccgcgccgtc tcgccagggt caggtcatcg gcaagcttcc cgcgaaatct
    10561 tggacgaggt ccgtctctcc tgtcggggga gtcgatcacg gtcgggcgcg gtggagggcg
    10621 cgtgaccttt ccggatcgat caatagaaaa gaataaatac tagacgagga cgtgtaacac
    10681 ctcgcccgcg atcttcgcca gtctctaggc tcgtctccgc tctggagacg ataacgacgg
    10741 ggaagctccc caggaggttc tcgctatggc cccttgtcga tatgatgaag attattcctg
    10801 ccggcctctc gttgatccct atggtccatg gcctcctcct tcgttggaca acgaccctct
    10861 gcaccaacct ccgcctccgg acgaatattg cctgcgggga ccgcaggtcg atcctggcgc
    10921 gagctactgg ctgctctgcc ctgcgccctg tgtggtgccc atccagcaga tcaccgaaat
    10981 caccgacaat ccggataacc cttgctattt cccgccgtac ccccccgagg acgtgacgaa
    11041 ggcggaggtc gccgagctca aggagctggc ctgtctgcgg gacgagcccg aggcgctctt
    11101 cagcgaccgg cgagggcgcg agcggaaggg gctcagcatg ttcctccggt tgcgtccgca
    11161 gccgttcgga gcggtgatca atacgctgcg gcggggcgac tcgcttctga tcaacggcgg
    11221 gccggagccg cggcgcgcgg cgatcgacgt ctgcgcccag ccacggcccg acatggggcc
    11281 gatcccctgc gccacggatt gcgtcgacga cttcttcccg gcgatccgga ccggccgtga
    11341 gctggcccgg tacttcgaga gcgaaacgcc gggattggcg catcgtctgg ccttgagcta
    11401 cctgctccgg gatgccaact attcgccgcc ctaccaggcc ttcatctggg cggcgctgga
    11461 cgtggcgatc tacagcgccc tcggggcggc ctggtattac aagtggatcg ccgactccaa
    11521 ctgcgagctt cgtgagccct gcaggccgat gagccggtac aggcgggaga ggacctcgcg
    11581 ccggccgcgc ccctacgagg tggactacga ggtcgacgtg ctgttcaatc gcgccgtcaa
    11641 ctgcaccaac tccggcgatg gggagcgccg cctgttgccg gacccctcgc cgggcacgcc
    11701 gcgccacccc tcctatccgt ccggccacag cacctacgcc ggcgccgcga gcgaaatcct
    11761 ttcctatttc ttccccgact actcggggga gctcacgaag ctggccgaca acacgggcat
    11821 gggccggctg tgggcgggca tccactaccg gtcggaccac atcgagggga tgaagctcgg
    11881 ccggtgcgtg gctcgtctga tcatccgtca gatcgagggc tcgtgtatcg tccggcccga
    11941 tccttgtgac agcgcggcgg cttccgtggc cgactgcaag aaggggccgc cttccagaga
    12001 gcacctgtgc cggtgcgccc aggagctctg cgagtgctgc gcgaggaggg gagcccgcca
    12061 cgcggcggcc ggtctccagg aggaatccgt cgccccgggt cggcagacgc aggaggggga
    12121 gggcggcgag gaaggggagg ggggcggtgc cggctcctcc gcggccgtgc gcgagcaggc
    12181 gcggggtccc caggagggcg cgggcgtcgc cggctccagc ggcgaggcgc gggagcaggc
    12241 ccaggggccc caggagggcg caggcaccgc cggctcctcg gagggagcgc gggagcaggc
    12301 caagggaccc caggaagggg ctcagtagaa gacgaggggc gcggcggccc ccggccgccg
    12361 cgccccatcc ggtgataccc tcccgggagg gaggcgcgcg agaggatgac caccgcgtac
    12421 atcgaccatg tcctcgtcgg tgttcccgac ctcgaggccg gaatcggcgc catggagggc
    12481 ctcatgggcg tgcggccggt gctcggaggg gagcatcccc actggggcac ccacaacgcc
    12541 ctgctctcgc tgggggacgg gatctacttc gagctgatcg cggtgcggcc gggcgcggag
    12601 gcgccgaagt tcgagcacct gaggtcgctg tccgctccca cgccgatcgg ttgggcggtg
    12661 cgggtcgacg atccccggga ggtctcgtcc ctcctccggg gtgccggctt ggctccgacc
    12721 gataccaagc cgggctcacg ccagacgccg gccggtgccg ttctgcgatg ggagaccttc
    12781 gatctcgatc cggcgccggc ggggtccccc ttcttcattc attggctgcc cgagacggcc
    12841 catccatcca agacctctcc aagcggctgc tccctcagtc gcttcgagat cgcctctcct
    12901 gacgccgcgg agctggtccg cctgcgtgac gcgctggggc tgcggctcga tatccgggag
    12961 gcggagactc cccgcatgac cttgaccctc cggtgcccca aggggacggt cacgctgccg
    13021 gcgacctgac cgacgaggca cccggccgga tctcagcatg tgaagcagac atcgatccac
    13081 tgaagtacga tctctcctga aaccttccag gctcggcgtg cgtaggattc tacattgtag
    13141 aatcacggct ccgccgaagg gaccctgacc gaccatgccg cacgccagcc accgcgcacc
    13201 cgacgacccg acgttgccga tccgcctcgc cgtctatctg ctgagcggac tgatccccgc
    13261 cgccgagcgg gacgaggtgg ccggcgacct gcgggacgag tacgcgcacc ggcttggcgc
    13321 gcaggggagg gcggctgccg ccgtctggct ctggcggcag gtgctgggct cggtgccggc
    13381 gctcctgcgg cggagctggt ggcgcggctg gaccggcttc gagccggcgg cgaaccgact
    13441 gctccctgga ggaacctgga tggaaagctg gatcatggat ctgcggtacg cggtgcggcg
    13501 cttgcgctcg cggccgacct atgtgctcct cgcggtgctg accctcgccc tcggcgtggg
    13561 ggggaccgcc gcgatcctca gtatcgcgcg cggactgctg gtcgatccgc tcccctatga
    13621 aaaggaggag gaggtcggcg tcttctggaa tccccatgac tggtccgagg cggagttcct
    13681 ctacctgcgg ccggagttcc ccggcttcag tcaggtggcc gcgtaccggc ccgaggacgt
    13741 gacgctggag cccagggagg cgccggcacg ggtgctgacg gggatctccg cgacggcgga
    13801 gctgttccag gtgctcggga cgcgccccgc gctcggccgt gtcttccagc cgggcgacga
    13861 ccggcagggg ggcgatccgg tggtcgtgat cagccatggg ttgtggcagg agatgggggg
    13921 cgacgcctcc gtcatcggcc gtcgcgtcca gctcgacggt atcccgcgga ccgtggtcgg
    13981 cgtgatgccg cgcggcttct ggttccccga tcccacagtg cgcgtctggc tcgccgagca
    14041 gctcgagccg acgaacggga gcggcatgta cgcgctggtc ggccgcgtcg cgccggggca
    14101 acggatcgag gggatcaccc ccacactcgc ccgcatcacc atggcgctcg gtgagcggtt
    14161 ccagtatccc gagcagtggg acaagacgaa gaacgccgcg gtcaccccca tccgcgagta
    14221 cctggtcggc tccctgcggc cggcgctcct ggcgacgctc gccgccatgg cggtcatcct
    14281 cctcatcgcc tgcgccaacg tcgcggcgct gatgctcgac caggtgaacc gccgcgccgg
    14341 cgagctggcg gtgcgctcct cgctgggcgc cgaccggggc cggatcacgc agcagctcgt
    14401 gatcgaagcg ctggtgatcg gcctccttgc cggcggtgcc ggcgcgctcc tcgctgcggg
    14461 cggcttccgg gtgctggccg gcgcgctgcc gctcggggcc tgggccgaga gcgcggagct
    14521 cgactggacg ctgttcggcg ccgccatcgg cctcgccctg ctcgcggcgc tcggcgtcgc
    14581 gctgattccg gcgatgtcac tctggcgcgg cgacctgcgc gacgccctga cgcgcgtgcg
    14641 gacggcgacg gcgggcggcc agggaggccg ggtcgagagt ggcctcgtgg tcgccgaggt
    14701 cgcgctcgcg gtgctcctgg ccgccggtgc tgcgctgatg atccgcagcg tcggcaacct
    14761 g
//
LOCUS       DASPJI010000735         9151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_678774, whole genome shotgun sequence.
ACCESSION   DASPJI010000735 DASPJI010000000
VERSION     DASPJI010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9151)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9151
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678774"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VEL74_20590"
     CDS             <1..662
                     /locus_tag="VEL74_20590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG64993.1"
                     /translation="SDTGTGMDKATMEKVFEPFFTTKEAGKGTGLGLATVHGIVHQSG
                     GDIRVESALGTGTRFTILLPRARAEAEPEPVRAVRPREEATGSETILLVEDEDNIRGP
                     AMEILESRGYTVLAASHAAEALAKAELHPGAIHLLVTDVIMPGMSGGQLAEHLARSRP
                     GVRVLYMSGYPEDAISHHGVLSPEQRFLQKPFAVGLLLRTVREVLDAERGAGGTGTGP
                     A"
     gene            673..1782
                     /locus_tag="VEL74_20595"
     CDS             673..1782
                     /locus_tag="VEL74_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017719995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HYG64994.1"
                     /translation="MSEPRPGHDILVIGASAGGVQALCELVAGLPEDLPAAVLVVLHV
                     SPHPSSALPAILTRCGRLPALHPEDCTPLQPGTVYVAPPDRHLTVERGQVCINRGPRE
                     NGHRPAVDVLFRTAARAYGNRVVGVVLTGNLDDGTAGLAAIKRLGGVAIVQDPEEADY
                     PGMPASAVENVAVDHIASLAEIPRLLAELAHQEVEPWEEEEEPVAEHEPSEDRAHLGV
                     DGGNPGEGYGPPSGLTCPDCGGALYDKQEETVIGFRCRVGHAYSPEALAAAQSEEIDA
                     ALWAAVRALEENAALAHRLAKRMAGAGKESLESRYSSRARGSERYAQTLRKILMQDEK
                     ELAGGGGNGDLAGGNSGSARAILTPRRSPRARNLE"
     gene            complement(1937..2887)
                     /locus_tag="VEL74_20600"
     CDS             complement(1937..2887)
                     /locus_tag="VEL74_20600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG64995.1"
                     /translation="MRKMCSGIGIALLAAVAPMAASAQELIIEHVPAPAKAVQMMERV
                     NAKLEAGGSDVRLSEASFFTVGQGTSSARVLRIGTRWPYEQLTYVLDASGFTADVPAA
                     DTEGALVSSFESWNNVNNTTVNVSRIADDGGNFDILDAVVLDAAGNCVDIYDVTSPNL
                     IGFSPTGGVFIEPEADIVVGGWLPASYFANCLGSANIIGITWTFSVPDQDGDNYRDTV
                     YVEQFYNEGFQFVTNGAAYLNFNLMDIESIAVHENGHALGLDHFGGPNTNQPFRLQPN
                     GKVYDPEAVMNPAYLGGTAKRTPLPTDEAGLRTLFGRKGN"
     gene            3044..3127
                     /locus_tag="VEL74_20605"
     tRNA            3044..3127
                     /locus_tag="VEL74_20605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3077..3079,aa:Leu,seq:taa)
     gene            3154..5091
                     /locus_tag="VEL74_20610"
     CDS             3154..5091
                     /locus_tag="VEL74_20610"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HYG64996.1"
                     /translation="MKSAGILIACLVLAAGVPASAWDQLDQEEEVLASAEELKGLSIE
                     ELMEIDVTSVTRRSASLSSAAAAISVITREDIRRSGVTSLPEALRLVATLHVARFDGR
                     TWGISARGFNITTANKMLVLIDGRSVYTPLFSGVFWDVQDTLLEDVDRIEVIRGPGAT
                     LWGANAVNGVINIITRPAGDTQGGLVTAGAGNEERAFGAVRYGGALGKRGHYRAYAKH
                     FDRDSLAFADGRDAEDPMKMGQGGFRADWQGAGEADWFTVQGDVYRGRVGEPTRADTD
                     VDGGNLLGRWSRKTSESSGMELQVYWDNTYRRVPTVFEEHLDTWDVDFQHQLRAAERH
                     NVIWGLGYRYSRDRVSDSPALAIPPPRRTLELASAFLQDEISLAGDRLRVTVGSKVEH
                     NQSTGFEIQPSVRFAWTPADDRTLWGAVSRAVRTPTRFDEDLVFFTTGGVPFIRGSRD
                     FESEELLAWELGYRWLPLRDLSLDLSAFYHVYDELRSQEPTPGTVLPIVLANKLNAET
                     WGLEGRANWQASQRWRVYFGWALFENDLSFDPDSRDASGGEAEGNDPDYRFTLRSQLD
                     LPGRFELDGWLRTVGELPSPEVPDYTELDLRLGWRATDVLELSLVGQNLLHDQHPEFG
                     PPGPARREVERSVYGKVLWRF"
     gene            5079..5639
                     /locus_tag="VEL74_20615"
     CDS             5079..5639
                     /locus_tag="VEL74_20615"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HYG64997.1"
                     /translation="MALLRRTVLLALLALVPLVPAALLGQRAASPEYQVKAAFLYNFT
                     KFVEWLDARPGERSPLRLCVLGNDPFGETLDGIVFGEMVGSRPLLPERVDAGEPLNSL
                     RSCQVLFVSRSERARVREVLESLAETPVLTVSDLQGFLDAGGHIQFVMQGNKVRFAID
                     QAAAERSRLRISSKLLRLALSPSEVR"
     gene            5644..7161
                     /locus_tag="VEL74_20620"
     CDS             5644..7161
                     /locus_tag="VEL74_20620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG64998.1"
                     /translation="MGFLRDRPIRQKLVLIIMLVTGEALLLASAGMIVSDVLRSRNDL
                     ARDLEIIAAIIADNSTAALQFDQAPDVAATLEALEAQRSIVTAAVYDEKRALFAEYRR
                     PGTSRPIPRQPDRGARRARFGDDSLALFRPIMLDGEEIGTVYLESDLSEIEESVRAQS
                     LTVLSVLVISGLLALALSSGLQRLISRPILALAETARAVSERRDYSIRARKTSSDELG
                     QLVEAFNDMLSQIQRRDSDLQSAKEELEHRVEERTLELRQELLERHRAERELAQTNVE
                     LEQSNKELDDFAYIASHDLKEPLRGIHNYSVFLMEDYADKLDADGRAKLETLTRLTRR
                     MEALIDSLLHFSRVGRVDLAVDQVDLNDIVAEVVDSIGINLKESDIQVRVPRPLPTVR
                     ADRARVGEIFYNLMTNAMKYNDKPEKWIEVGCKEGTRPPVFYVRDNGIGIPQKHIDSV
                     FRIFKRLHAREKFGGGTGAGLTIAKKIVERHNGRIWVESAQGQGTTFYFTLDQGA"
     gene            7162..7608
                     /locus_tag="VEL74_20625"
     CDS             7162..7608
                     /locus_tag="VEL74_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG64999.1"
                     /translation="MTTPNQPILLVEDSPEDFEATERALRKAGLKNPIYRCSDGEEAL
                     DFLHRRGSYGEGADAPRPGIVLLDLNLPGTDGREVLAEIKGDPALKQIPVVVLTTSND
                     QRDIDACYKSGANSYIQKPVDMDGFIKAIERLHGYWLEVVILPKGE"
     gene            7612..>9151
                     /locus_tag="VEL74_20630"
     CDS             7612..>9151
                     /locus_tag="VEL74_20630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015183826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG65000.1"
                     /translation="MAEPLLILIVDDSLEDREVYRRLLLQDRDHEYRFQEAESGEEGL
                     EAAAMEAPDCILLDYRLPDVDGLEFLERVGGEAVPVIVLTGQGSEAVAVEAMKNGAQD
                     YLLKGSVTREGLQRAIHNAVEKVALRREVEARTAELARANHDLTREIQERRRAEEALQ
                     VMNDELEVLVQQRTAELSRAHSREKEARRQAEEANRLKDEFLATLSHELRTPLNAILG
                     WAQMLRSGKLDAATTGRAVETIERNAKAQAQLIADILDVSRIITGKLRLELGPVNLPR
                     VIETVLDSVRPAAEAKEIRLSVVLDPLATPVLGDSDRLQQVIWNLLSNAIKFTPKGGL
                     VGVVLRQVDDSGLEIEVRDSGIGIRPDFLAYVFDRFRQAESSITRSHGGLGLGLSIVR
                     HLVELHGGTVHVDSEGEGKGAAFTVRLPVRAQGAGAGEALAGRRSMDDEAAGVASAGP
                     PPLAGVHVLVMDDEEDTRELVAMALSHGGATVTAAASVPEALEALDRSVPDVLVSDIG
                     VPGED"
BASE COUNT         1422 a         3105 c         3303 g         1321 t
ORIGIN      
        1 tgtcggacac cggaaccgga atggacaagg ccacgatgga gaaggtgttc gagccgttct
       61 tcaccaccaa ggaggccggc aaggggacgg ggctcggcct cgccacggtg cacggcatcg
      121 tccaccagag cggcggcgac atccgggtgg agagcgcttt gggaaccggc acccgtttca
      181 ccatcctcct tccccgggcc cgcgccgagg cggagccgga gccggtgcgg gccgtccggc
      241 cgcgcgagga ggccaccggc tccgagacca tcctgctggt ggaggacgag gacaacatcc
      301 gcggcccggc gatggagatc ctggagagcc ggggctatac ggtgctcgcc gcctcccatg
      361 ccgcggaggc cctggccaag gccgagctgc atcccggagc gatccacctg ctggtgactg
      421 acgtcatcat gcccgggatg agcggcggcc agctcgccga gcacctggcg cgcagccgcc
      481 cgggcgtcag ggtgctctac atgtccggct accccgagga cgccatctcc catcacggcg
      541 tgctcagccc cgagcagcgc ttcctgcaga agcccttcgc cgtcggcctg ctgctgcgga
      601 cggtgcggga ggtcctggac gcggagcggg gagccggggg caccggcacg ggccctgcat
      661 gacgggttct gaatgagcga gcctcgtccg ggccatgaca ttctcgtgat cggcgcttcg
      721 gccggcggcg tccaggcgct gtgcgaattg gtcgccggcc tgcccgagga tctgcctgcc
      781 gccgtcctcg tcgtcctcca cgtgtcgccg catcccagca gcgcgctccc cgccattctg
      841 acccgttgcg gccggctgcc ggccctgcat cccgaggact gtacccccct gcagcccggg
      901 acggtctacg tggccccccc ggaccgccac ctcacggtgg agcggggaca ggtctgcatc
      961 aaccgcgggc cgcgcgagaa cggccaccgg cccgcggtcg acgtgctgtt ccgcaccgcc
     1021 gcgcgggcct acgggaaccg ggtggtgggg gtggtcctca ccggcaacct cgacgacggc
     1081 accgccggcc tggccgcgat caagcggctc ggcggcgtgg cgatcgtcca ggacccggag
     1141 gaggccgact accccggcat gccggcgagc gccgtcgaga acgtcgccgt ggatcacatc
     1201 gcgtcgctgg ccgagatccc gcgcctcctg gcagagctgg cacatcagga ggtggagccg
     1261 tgggaagaag aggaggagcc tgtggcggag catgagccga gcgaggaccg cgcccatctg
     1321 ggcgtcgacg gcggcaatcc gggcgagggc tatggaccgc cgtcggggct cacctgtccc
     1381 gactgcggcg gcgccctgta tgacaagcag gaggagacgg tgatcggctt ccgctgccgg
     1441 gtcggtcacg cctactcccc cgaggccctg gccgccgccc agtccgagga gatcgacgcc
     1501 gccctctggg ccgccgtgcg cgccctggaa gagaacgccg ccctcgccca ccgcctggcg
     1561 aagcgtatgg ccggagcggg caaggagtcc ctggagagcc gctacagcag ccgcgcccgc
     1621 ggctcggaga ggtacgccca gaccttgcgc aagatcctga tgcaggacga gaaggagctg
     1681 gcgggcggcg gcggcaacgg cgacctcgcg ggaggcaatt ccggatccgc ccgggccatc
     1741 ctcacaccgc ggcggtcgcc gcgggcgcgg aacctggagt aggagacccc cgacaccccc
     1801 tacgcgctga cttcgggccg cagggggcgg tggtgcctcc ccccctccct tccgccccgt
     1861 gaaaccaaca acttcttggc tgaagcagaa aagggggccc gaaggccccc tctgccgggt
     1921 gctcctgggt ggagcctcag ttgcccttgc ggccgaacag ggtgcgcagg ccggcctcgt
     1981 cggtgggcag cggggtccgc ttggcggtgc cgccgaggta ggcggggttc atcaccgcct
     2041 cggggtcgta gaccttgccg ttgggctgca gccggaacgg ctggttggtg ttcgggccgc
     2101 cgaagtggtc gaggcccagc gcgtggccgt tctcgtgcac cgcgatcgac tcgatgtcca
     2161 tcaggttgaa gttcaggtag gcggcgccgt tggtcacgaa ctggaagcct tcgttgtaga
     2221 actgctcgac atagacggtg tcacgataat tgtcaccatc ctggtcgggc accgagaagg
     2281 tccaggtgat gccgatgatg ttggcgctgc ccaggcagtt ggcgaagtag gacgccggca
     2341 gccagccgcc gaccacgatg tccgcctcgg gctcgatgaa cacgccaccc gtcgggctga
     2401 acccgatcag gttgggcgag gtgacgtcgt agatgtcgac gcagttgccc gccgcgtcga
     2461 gcacgacggc gtcgaggatg tcgaagttgc cgccgtcgtc ggcgatgcgg ctgacgttaa
     2521 ccgtggtgtt gttgacgttg ttccagctct cgaaggagct caccagcgcc ccctcggtgt
     2581 ccgccgccgg gacgtcggcc gtgaagccgg aggcgtccag cacgtaggtg agctgctcat
     2641 agggccagcg ggtgccgatg cgcagcacgc gggcgctgct cgtgccctgg ccgacggtga
     2701 agaaggaggc ctcggagagg cggacgtcgc tgccgccggc ctcgagcttg gcgttgaccc
     2761 gctccatcat ctggacggcc ttggccgggg cggggacgtg ctcgatgatc agctcctggg
     2821 cggaggcggc catcggcgcg acggcggcaa ggagggcgat tccgattcct gaacacattt
     2881 tgcgcattaa cagttctcct tacagaggtg tacagtcaag aattacagcc taccgaattg
     2941 ccggggggca tacgcccgac attttagctc aagagccttg agctgcaaca gggggaggcg
     3001 ggccggcatt ccctgcccgg gccggctgct acaatgcggc gcggccggag tggcgaaatg
     3061 gcagacgcag gagacttaaa atctctcgcc gcaaggcgtg cgggttcgag tcccgcctcc
     3121 ggcaccagcg ccgaccgtag aaaaggagtg tgcttgaaga gcgcagggat cctcatcgcg
     3181 tgcctcgtcc tcgccgccgg cgtgccggcc tcggcctggg atcagctcga ccaggaggag
     3241 gaggtcctcg cgtcggcgga ggagctcaag ggcctgagca tcgaggagct gatggagatc
     3301 gacgtgacgt cggtcacgcg gcgcagcgcc agcctgtcca gcgcggcggc ggcgatctcg
     3361 gtcatcaccc gggaggacat ccggcgctcc ggcgtgacca gcctgcctga ggccctgcgt
     3421 ctggtggcga ccctgcacgt ggcgcgcttc gacggccgca cctgggggat cagcgcccgc
     3481 ggcttcaaca tcaccacggc caacaagatg ctggtgctga tcgacggccg cagcgtctac
     3541 acgccgctct tctcgggcgt cttctgggac gtccaggaca cgctgctcga ggacgtggac
     3601 cgcattgagg tgatccgcgg ccccggggcc accctctggg gggcgaacgc ggtcaacggc
     3661 gtgatcaaca tcatcacccg cccggccggt gacacccagg gggggctcgt gaccgccggc
     3721 gccggcaacg aggagcgggc cttcggcgcg gtgcgctacg gcggcgccct ggggaagcgg
     3781 ggccactacc gggcctatgc caagcacttc gaccgcgact ccctcgcctt cgccgatggc
     3841 cgtgacgccg aggacccgat gaagatgggg cagggcggct tccgcgccga ctggcagggg
     3901 gcgggggagg ccgactggtt caccgtgcag ggggacgtct accgcggccg ggtcggcgag
     3961 ccgacccgcg ccgacaccga cgtcgacggc ggcaacctcc tggggcgctg gagccgcaag
     4021 acctcggaat cctccggcat ggagctgcag gtgtactggg acaacaccta ccgccgggtg
     4081 cccaccgtct tcgaggagca tctcgacacc tgggatgtgg acttccagca ccagttgcgg
     4141 gcggcggagc gccacaacgt gatctggggc ctgggctacc gttacagccg cgaccgcgtg
     4201 agcgacagcc ccgccctcgc catcccgccg ccccggcgca ccctggagct ggcgagcgcc
     4261 ttcctgcagg acgagatctc gctggccggc gaccgcctgc gcgtcaccgt cggctccaag
     4321 gtggagcaca accagtccac cgggttcgag atccagccga gcgtgcgctt cgcctggacc
     4381 ccggcggacg accgcaccct ctggggggcg gtgtcgcggg ccgtgcgcac ccccacccgc
     4441 ttcgacgaag acctggtctt cttcaccacc gggggcgtgc cgttcatccg cggcagccgc
     4501 gacttcgagt ccgaggagct gctggcctgg gagctgggct accgctggct gccgttgcgt
     4561 gacctgtcgc tggacctgtc ggccttctat cacgtctatg acgagctgcg ctcgcaggag
     4621 ccgacgcccg gcaccgttct tcccatcgta ctggccaaca agctgaacgc cgagacctgg
     4681 ggcctcgagg ggcgggcgaa ctggcaggcg agccagcgct ggcgcgtgta tttcggctgg
     4741 gccctgttcg agaacgacct gtctttcgac cccgacagcc gcgatgccag cggcggcgag
     4801 gcggagggga acgaccccga ctatcgattc accctgcgct cgcagctcga cctgcccggc
     4861 cgcttcgagc tggacggctg gctgcgcacc gtcggcgagc tcccctcgcc ggaggtgccg
     4921 gactacacgg agctcgacct tcgcctgggc tggcgcgcca ccgacgtcct ggagctgtcg
     4981 ctcgtcggac agaacctgct gcacgaccag cacccggagt tcggaccgcc gggccccgcc
     5041 cgccgcgagg tggagcggag tgtctacggg aaggtgttgt ggcgcttctg aggcgaaccg
     5101 tcctcctggc gctcctcgcg ctcgtgcctc tggtccccgc cgccctgctg gggcagcgcg
     5161 ccgcgtcgcc cgagtaccag gtcaaggcgg cgttcctcta caacttcacc aagttcgtgg
     5221 agtggctcga tgcccgcccg ggggagcgct cgccgctccg cctctgcgtc ctcggcaacg
     5281 acccgttcgg cgagaccctc gacggcatcg ttttcggcga gatggtgggt agccggccgc
     5341 tgctgcccga gcgcgtcgac gccggcgagc cgctgaacag cctgcgctcc tgccaggtgc
     5401 tgttcgtcag ccggtcggag cgggcgcggg tacgggaggt cctggagtcc ctcgccgaga
     5461 ccccggtgct gacggtcagc gacctccagg gcttcctcga cgccggcggg cacatccagt
     5521 tcgtcatgca ggggaacaag gtgcgcttcg ccatcgacca ggccgcggcc gagcggtcgc
     5581 ggctgaggat cagctcgaag ctgctgcgcc tcgccctgtc gccgagtgag gtccgctgac
     5641 gggatggggt tcctgcgcga ccgcccgatc cgccagaagc tggtgctgat catcatgctg
     5701 gtcaccggcg aggcgctgct cctggcgagc gccggcatga tcgtctcgga cgtgctgcgc
     5761 tcgcgcaacg atctggcccg cgacctggag atcatcgcgg cgatcatcgc cgacaacagc
     5821 accgccgccc tgcagttcga ccaggccccg gacgtggcgg cgaccctgga ggcgctcgag
     5881 gcgcaacgct cgatcgtcac cgcggcggtc tatgacgaga agcgcgccct gttcgccgag
     5941 taccggcggc cggggacgtc gcgcccgatc ccccgccagc cggacagggg ggccaggaga
     6001 gcccgcttcg gcgacgattc cctggcgctg ttccgtccca tcatgctgga cggggaggag
     6061 atcggcaccg tctatctgga gagcgacctg tcggagatcg aggagagcgt gcgggcgcag
     6121 agcctgacgg tgctctccgt gctcgtgatc tcgggcctcc tcgccctggc cctctcctcg
     6181 gggttgcagc ggctgatctc gcggccgatc ctggcgctgg cggagacggc gcgggcggtc
     6241 tcggagcggc gcgactactc gatccgcgcc cgcaagacca gctccgacga gctcggccaa
     6301 ctggtggagg ccttcaacga tatgctctcc cagatccagc ggcgggattc ggatctgcag
     6361 agcgccaagg aggagctgga gcaccgggtg gaggagcgga cgctggagct gcggcaggag
     6421 ctgctggagc gccaccgggc cgagcgcgag ctggcgcaga ccaacgtcga gctggagcag
     6481 agcaacaagg agctggacga cttcgcctac atcgcctcgc acgacctcaa ggagccgttg
     6541 cgcggcatcc acaactactc ggtcttcctg atggaggact acgccgacaa gctggacgcc
     6601 gacggccgcg ccaagctcga gacgctgacc cgcctgaccc gccggatgga ggcgctcatc
     6661 gactcgctgc tgcacttctc gcgcgtcggc cgggtggacc tggcggtcga tcaggtggac
     6721 ctgaacgaca tcgtggccga ggtggtggac tcgatcggca tcaacctgaa ggagtcggac
     6781 atccaggtcc gcgtcccccg gcccttgccg acggtgcgcg ccgaccgggc ccgggtgggg
     6841 gagatcttct acaacctgat gaccaacgcg atgaagtaca acgacaagcc ggagaagtgg
     6901 atcgaggtgg gctgcaagga ggggacccgt cccccggtct tctacgtgcg ggacaacggc
     6961 atcggcatcc cgcagaagca catcgactcc gtcttccgga tcttcaagcg cctgcacgcc
     7021 cgcgagaagt tcggcggcgg caccggcgcg ggcctgacga tcgccaagaa gatcgtcgag
     7081 cggcacaatg gccggatctg ggtcgagtcg gcccagggcc aggggacgac cttctatttc
     7141 acgctggacc aaggagcctg agtgaccaca ccgaaccagc ccatcctcct ggtggaggac
     7201 agccccgaag acttcgaggc caccgagcgc gcgctgcgca aggccggcct caagaacccg
     7261 atctatcgct gctctgacgg tgaggaggcg ctcgatttcc tgcaccggcg ggggagctac
     7321 ggcgagggcg ccgacgcgcc gcgccccggc atcgtcctgc tggacctgaa cctccccggc
     7381 accgacgggc gcgaggtgct ggccgagatc aagggcgacc cggccctcaa gcagatcccg
     7441 gtcgtggtcc tcaccacctc caacgaccag cgggacatcg acgcctgcta caagtcgggg
     7501 gcgaacagct acatccagaa gccggtggac atggacggct tcatcaaggc gatcgagcgc
     7561 cttcacggct actggctgga ggtcgtgatc ctgcccaagg gggaatgacg catggccgag
     7621 ccgctcctca tcctcatcgt cgacgacagc ctggaggacc gcgaggtcta ccggcgtctc
     7681 ctgttgcagg accgcgacca cgagtaccgg ttccaggagg cggagtcggg tgaggagggg
     7741 ctggaagcgg cggccatgga ggcgccggac tgcatcctcc tcgattaccg gctgccggac
     7801 gtggacggcc tggagttcct cgaacgggtg ggaggggagg cggtgccggt gatcgtgctc
     7861 accggccagg ggagcgaggc ggtggcggtc gaggcgatga agaacggcgc ccaggattac
     7921 ctgctcaagg gctcggtgac gcgcgagggg ttgcagcgcg ccatccacaa cgccgtcgag
     7981 aaggtggccc tgcgccgtga ggtggaggcg cgcaccgccg agctggcgcg ggccaaccac
     8041 gacctgacgc gggagatcca ggagcgccgg cgcgccgagg aggcgctgca ggtgatgaac
     8101 gacgagctgg aggtgctggt ccagcagcgg acggcggagc tctcccgcgc ccactcccgg
     8161 gagaaggagg cgcggcgcca ggcggaggag gccaaccggc tcaaggacga gttcctggcc
     8221 accctctccc acgagctgcg gacgccgctc aacgccatcc tgggctgggc gcagatgctg
     8281 cgctccggca agctcgacgc cgcgaccacc ggccgcgccg tcgagaccat cgagcgcaac
     8341 gccaaggcgc aggcgcagct catcgccgac atcctcgacg tgtcgcgcat catcaccggc
     8401 aagctccgcc tggagctggg gccggtgaac ctgccgcggg tgatcgagac ggtgctggac
     8461 agcgtgcgcc cggcggccga agcgaaggag attcgcctgt cggtggtcct cgatccgctg
     8521 gcgacgccgg tcctgggcga ctcggaccgc ctgcagcagg tgatctggaa cctgctgtcg
     8581 aacgccatca agttcacgcc gaaggggggg ctggtggggg tggtgctgcg gcaggtggac
     8641 gactcgggcc tggagatcga ggtgagggac agcggcatcg gcatccggcc ggacttcctg
     8701 gcctacgtct tcgaccgctt ccggcaggcg gagagctcga tcacccgctc gcacggcggg
     8761 ctgggcctgg ggctgtccat cgtccgtcac ctggtggagc tgcacggcgg taccgtgcac
     8821 gtcgacagcg agggggaggg gaagggggcg gccttcacgg tgcgcctgcc ggtgcgcgct
     8881 cagggggcgg gagcggggga ggcactggcc ggccgccgct cgatggacga cgaggcggcc
     8941 ggcgtggcgt ccgccgggcc gccgccgctc gccggcgtgc acgtgctggt gatggacgac
     9001 gaggaggaca cccgggagct ggtggccatg gcgctgtcgc acggcggcgc cacggtgacc
     9061 gcggccgcct cggtgcccga ggcgctcgag gcgctggacc gttcggtgcc ggacgtgctg
     9121 gtgtccgaca tcggagtccc cggcgaggac g
//
LOCUS       DASPJI010000736        10172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_614548, whole genome shotgun sequence.
ACCESSION   DASPJI010000736 DASPJI010000000
VERSION     DASPJI010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10172)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10172
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614548"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VEL74_20635"
     CDS             complement(<1..323)
                     /locus_tag="VEL74_20635"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG65001.1"
                     /translation="MVSVERLLPVSLRPAAPRDAELLRRWRSEPSVRRFQPLNELPTS
                     QLRADVASQRMADLYRGRGEKFQWIVEVEGDPAGWITLVVSNWEHGLAEVGYALSTPY
                     QSRGV"
     gene            complement(398..1921)
                     /locus_tag="VEL74_20640"
     CDS             complement(398..1921)
                     /locus_tag="VEL74_20640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013942184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG65002.1"
                     /translation="MSGGPAVTIPGGPVLLALYYSVLAILAFYGVHRLVLVALYLKTR
                     KAGPPRPADPAEWPVVTVQLPLYNEMYVAARLIEAVARLDYPRDRLEIQVLDDSTDET
                     RDIAARAVEEQRRRGVDIHHIHRGDRRGFKAGALAAGLARARGELLAVFDADFVPQPG
                     FLKQAVPYFADPGLGLVQGSWSHINRNYSLLTRIEAILLDGHFLIEHTARNRSGCFFN
                     FNGTAGIWRRQAILDGGGWQHDTLTEDLDLSYRAQLAGWKFLYLPELAVPSELPVDVN
                     GFKSQQYRWTKGSIQTGRKLLGRILRSPLPFRVKLEAFVHLTNNACYALMVLLALLIF
                     PAMVMRRGTAAHMLLLVDLPLFLAATASVLAFYLVSQDAAGMRWRQQLRYLPAVMGMG
                     IGLSVNNARAVLSGLVRRGGTFHRTPKYRIERAGEDWRGKRYRAGASASLAVEVLLAV
                     YLAGCTVYAFWVGMWMSIPFLYLFVQGYGYITLLALWPRLSGPRLRPAPELPEAPGL"
     gene            1870..3297
                     /locus_tag="VEL74_20645"
     CDS             1870..3297
                     /locus_tag="VEL74_20645"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HYG65003.1"
                     /translation="MPAEPARRGWSRPDLRSFRVLLPLLAAHLGLLLVFDLARHLVLS
                     LSLLAAAFLWLPVAARRLAAVPRPGGLILLGALLLRLLLLPLPPVLSDDVLRYLWDGK
                     VAAAGLNPYALEPGAPELSALRDEVTWRVPHPEIATVYPPLALAAFSIASRFPFPVLI
                     WKLLATAADLGACALLLHLARRRGTPEGRVAWYAWNPLPVLEVAGMGHVDALGVAAVV
                     GVVLALTPRSREEEPEAGTRLPWGPGAWAAAAVLAKLVPVLAFPLWARRSGRPFRFLL
                     VALALTAVALLPVAVGTGGVPPGLVKYGVSWEFNGPLYEPLWRLLDEAGLAPWIAGRL
                     EANEAKTQDWYKWDPVYPYLYPQLLAKLLLGVGVLVTVALSLRERDLAAGTGRLFGRL
                     MLCAATVYPWYLLWVLPWAALEGWTAWRLLSGLILLSYLAGLGGLPIWPWGWLLIWGP
                     FAVVGAVTASTSSRTGRYHRASRHR"
     gene            complement(3239..3535)
                     /locus_tag="VEL74_20650"
     CDS             complement(3239..3535)
                     /locus_tag="VEL74_20650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65004.1"
                     /translation="MPRRQIFLTAVWSEEWKASYQALSPDRQTACDEAAIALIKQVDS
                     PGLRIKPIHPEKHYLEARINSGDRIVFRVEGGTIFFIDVVKHDDIGRYGKRSKR"
     gene            complement(3543..3893)
                     /locus_tag="VEL74_20655"
     CDS             complement(3543..3893)
                     /locus_tag="VEL74_20655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HYG65005.1"
                     /translation="MGKWYNPTLEVARLGDTVSFLTVDSKGRATLPEEVRSALGLKAG
                     DLVLLERTDRGTFELVPAALIPRDQLWFHHPEMQARLNRAEADVAEGRSTRTETPEQA
                     QAFLDSLKKSTAAR"
     gene            complement(4003..4221)
                     /locus_tag="VEL74_20660"
     CDS             complement(4003..4221)
                     /locus_tag="VEL74_20660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65006.1"
                     /translation="MPKKILKLTLALGMLVGGFTVGPSVRDVSAAGYCSDICCNASCT
                     SIRRCFSAGGGCLCRAYCEPNFGGGIER"
     gene            4363..5142
                     /gene="truA"
                     /locus_tag="VEL74_20665"
     CDS             4363..5142
                     /gene="truA"
                     /locus_tag="VEL74_20665"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HYG65007.1"
                     /translation="MTYRLTVSYRGAAYAGWQRQDNARTVQEVLEEALSALLGRTVRV
                     VGASRTDAGVHARGQVAHLELDAPFPERGLIHGTNHHLPEDVRVLAAARVPDGFHARK
                     HALGKTYSYRLSRAAVLSPLDSLFVAQVPDRLDLAAMREAAAHLPGRHDFSAFALAGG
                     AHGQPFRTILAARWEEMGDELRFRILGEGFLRGMVRALVGTLIEVGLGRRTPGDFAGL
                     LDGRPRAAAGPTAPAHALVLERVFYPPEWQTPGDPPDVMVL"
     gene            5156..5974
                     /locus_tag="VEL74_20670"
     CDS             5156..5974
                     /locus_tag="VEL74_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HYG65008.1"
                     /translation="MADLSKLADPGSPEELLVRRIDMARLPRHVAIIMDGNGRWAKAK
                     SLPRVEGHRAGVAAVRDTVETAARLELEGLTLYAFSVENWKRPRFEIWTLMNLLKEYV
                     RKEREALVDNDIRFNVLGRWEELDPSVVRELRTTLEATRHCQGMQFNIALNYSGRCEI
                     VDACRRIVADWAAGKHADIDEETIGRYLYTSGQADPDLLIRTSGEMRISNFLLWQIAY
                     TEIWVTRTLWPDFRRRHLYEAILDFQGRERRYGGVDQTGEAEQPDLVASAHRQA"
     gene            5991..6845
                     /locus_tag="VEL74_20675"
     CDS             5991..6845
                     /locus_tag="VEL74_20675"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HYG65009.1"
                     /translation="MQRLLTAAVATPLVLAAVFLLPGLWFFLFVALAIELAVYEYITI
                     VRHRAPNAPLRLLLVLVPLASLGIWWWLSGEASPVQPGLPLLVGAVLMSVGVGTLVLF
                     GKTPLEETLTTLGIFGFGIPYFAVAVASTVGIQQTADPGGVPNHGPWLIVLLFAIVWL
                     GDTAAYYIGSRWGRHKMAPVISPKKSWEGAAAGFVTGLLAAVAWSYLRLGRLEPRLLV
                     VAAVTAAAAQIGDLVESMIKRGAGVKDSGSILPGHGGFLDRLDALLFAAPVLLLALWV
                     LGLHGMTR"
     gene            6942..8258
                     /gene="rseP"
                     /locus_tag="VEL74_20680"
     CDS             6942..8258
                     /gene="rseP"
                     /locus_tag="VEL74_20680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HYG65010.1"
                     /translation="MNLLTNALAFIFALGIIIFVHEGGHLMMAKAFKVRVMTFSLGFG
                     KRLWGFTRNGTDYRVSAIPFGGYVRMGGENPEESTGDPNEFLSKPRWQRILVYLAGPA
                     MNFILAIGLFAILFMVGIEVPNLPDTPPLIGGVQEGSSAAQAGLRRGDLIVSIKGEEV
                     DSWQDALTELLTSAGRPVPLGIKRGEQTFTAVVTPTKDPRSELGDLAGLYPSFRPQIT
                     HVAPGGPAEKAGLKPGDEIRSIDGNPVADSRDFVSYIEKRPGQPIRLQIMRDGRPLEI
                     PVTPQLNGTVGRVGIQLGIFQRYAPLRAVQEGARYSLELVRQTFQIIGKIFNREVSAK
                     GALAGPIEIAAQSGAAAREGFKTLVHLMGFLSIGIAIMNLMPIPILDGGQTLILMVEG
                     VIRRDLSLRLKEAISHVGFILILLLTVMVLWFDLLKHMPEGLIPGS"
     gene            8294..8824
                     /locus_tag="VEL74_20685"
     CDS             8294..8824
                     /locus_tag="VEL74_20685"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HYG65011.1"
                     /translation="MPRPSSSVSIGDEAPNFDLSSTENAVLMLKDELARTSVLLYFFA
                     SAESDRVRRDLEALERRREALKGLRGRILGVSPLTVDALRKVQADSRLRFPLLCDDRN
                     FSASYGVGPIEEGRPADPALVLVSRKQKILFVANPVAAVEEALPQVEKLLKDLPSPTR
                     DYPKSVINRWIDRWVN"
     gene            8863..9063
                     /locus_tag="VEL74_20690"
     CDS             8863..9063
                     /locus_tag="VEL74_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65012.1"
                     /translation="MSNVEMLEAQIQKLSPTELAEFRDWFAAYDAEAWDRQFEKDVEA
                     GKLDALAEQALRQRAAGLCTDL"
     gene            complement(9954..>10172)
                     /locus_tag="VEL74_20695"
     CDS             complement(9954..>10172)
                     /locus_tag="VEL74_20695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65013.1"
                     /translation="DRHAHIGDGEIGVDLFAWLVTEPRLAAIPMVLETEPGDDMKGHR
                     RDLNTLRSLAGAPALKRRRRKKAFSKRS"
BASE COUNT         1502 a         3550 c         3493 g         1627 t
ORIGIN      
        1 atcacgcccc gggactgata gggggtggag agggcatagc cgacctcggc gaggccgtgc
       61 tcccagttgc tgaccaccag ggtgatccag ccggcggggt ccccctcgac ctcgacgatc
      121 cactggaact tctcgccgcg gccgcggtag aggtcggcca tgcgctggct ggcgacgtcg
      181 gcccgcagct gggaggtggg cagctcgttg agcggctgga agcgccgcac gctgggctcg
      241 gagcgccaac gccggaggag ctcggcgtcc cggggagccg cgggacgcag gctcaccggg
      301 aggaggcgtt ccactgaaac catggagcca ttttagcccc tggacccgag atccagaaga
      361 ttgcacttcc ggaaatagaa gattgcactt ccaggcgtta aaggcccggc gcctcgggca
      421 gctcgggggc cgggcggagc cgagggccgg agaggcgcgg ccagagggcg agaagggtga
      481 tgtagccgta cccctgcacg aagaggtaga ggaaggggat ggacatccac atcccgaccc
      541 agaaggcgta gacggtgcag ccggcgaggt agacggcgag cagcacctcc accgccagcg
      601 aggcgctggc gccggcccgg tagcgcttgc cccgccagtc ctcccccgcc cgctcgatcc
      661 ggtacttggg cgtgcggtgg aacgtgccgc cgcgccgtac cagcccggag agcacggcgc
      721 gggcgttgtt gaccgacagg ccgatgccca tccccatcac cgccggcagg tagcggagct
      781 gctgccgcca gcgcatgccg gcggcgtcct ggctcaccag gtagaaggcg agcaccgaag
      841 cggtcgccgc caggaacagc ggcaggtcca ccagcagcag catgtgggcc gcggtccccc
      901 ggcgcatcac catcgccggg aagatgagga gggccaggag gaccatcagc gcgtagcaag
      961 cgttgttggt caggtggacg aaggcctcca gcttgacccg gaacggcagg ggggagcgca
     1021 agatccgccc cagcagcttg cggccggtct ggatggagcc cttggtccac cggtactgct
     1081 ggctcttgaa gccgttgacg tcgaccggca gctcggaggg cacggcgagc tcgggcaggt
     1141 agaggaactt ccagccggcg agctgcgcgc ggtaggagag gtcgaggtcc tcggtcaggg
     1201 tgtcgtgctg ccagccgccg ccgtcgagga tcgcctggcg ccgccagatg ccggcggtgc
     1261 cgttgaagtt gaagaagcag ccgctgcggt tccgggcggt gtgctcgatg aggaaatggc
     1321 cgtcgagcag gatcgcctcg atgcgggtga ggagcgaata gttgcggttg atgtgggacc
     1381 agctcccctg gaccagaccg agccccgggt cggcgaagta cggcaccgcc tgcttgagga
     1441 agccgggctg ggggacgaag tcggcgtcga agacggcgag cagctcgccc cgcgcccgcg
     1501 ccaggccggc ggcgagggcg ccggccttga agccgcggcg gtcgccgcgg tggatgtggt
     1561 ggatatcgac gccgcggcgc cgctgctcct cgacggcgcg ggcggcgatg tcgcgggtct
     1621 cgtcggtcga gtcgtcgagg acctggatct ccagccggtc gcgcgggtaa tcgaggcggg
     1681 cgacggcctc gatgaggcgc gccgccacgt acatctcgtt gtagagtggg agctgcaccg
     1741 tgacaacggg ccattccgcc gggtcggcgg gccggggagg cccggccttc cgggtcttca
     1801 gatagagcgc caccagcacg aggcgatgga cgccgtagaa ggcgaggatc gccaggacgg
     1861 agtaatacag tgccagcaga accggcccgc cggggatggt cacggccgga cctccgctca
     1921 tttcgggttc tcctgccgct gctggcggcc cacctcggcc tgctgctggt cttcgacctc
     1981 gcgcgtcatc tggtcctgtc cctgtctctc ctggcggcgg ccttcctgtg gctgccggtg
     2041 gcggcgcggc ggctcgcggc ggtgccgcgg cccggcgggc tgatcctgct gggggccctg
     2101 ctgctgcggc tgctcctcct gccgctcccg cccgtgttgt cggacgacgt gctgcgctac
     2161 ctctgggatg gcaaggtggc cgccgccggg ctgaacccct acgccctgga gccaggggct
     2221 ccggagctgt ccgccttgcg ggacgaggtc acgtggcgcg tcccccaccc ggagatcgcc
     2281 accgtctatc cgccgctcgc gctcgcggca ttcagtatag cgagtcgctt tccctttccg
     2341 gtcctgatct ggaagctgct ggcgaccgcc gccgacctgg gggcctgcgc cctgctgctg
     2401 catctcgccc gccgccgcgg cacgcccgag gggagggtcg cctggtacgc ctggaacccg
     2461 ttgcccgtcc tggaggtggc ggggatgggc cacgtcgacg ccctcggggt ggcggcggtg
     2521 gtgggggtgg tgctggcgct caccccgcgc tcccgggaag aggagccgga ggccgggacg
     2581 cgcctcccct ggggccccgg cgcctgggcc gcggcggcgg tcctcgccaa gctggtgccg
     2641 gtcctggcct tccccctctg ggcgcggcgg agcggccgcc ccttccgctt ccttctggtg
     2701 gcgctggccc ttaccgccgt ggcgctcctg ccagtggcgg tggggaccgg cggggtcccc
     2761 cccggcctgg tcaagtacgg cgtctcctgg gagttcaacg gccccctcta cgagccgctc
     2821 tggcgcctgc tggacgaggc cggcctggcc ccctggatcg ctggccggct ggaggccaac
     2881 gaggcgaaga cccaggactg gtacaagtgg gaccccgtct atccttatct ctacccgcag
     2941 ctcctggcga agctgctcct cggcgtcggg gtcctggtga cggtggcgct gtccctgcgc
     3001 gagcgcgacc tcgccgccgg caccgggcgc ctctttggcc ggctgatgct gtgcgcggcc
     3061 accgtctacc cctggtatct cctctgggtc ctcccctggg ccgccctgga aggctggacc
     3121 gcctggcggc tgctctccgg gctgatcctc ctctcctacc tggccgggct ggggggcctg
     3181 ccgatctggc cctgggggtg gctgctgatc tgggggccgt tcgcggtggt gggggcggtt
     3241 accgcttcga cctcttcccg taccggccga tatcatcgtg cttcacgaca tcgatgaaga
     3301 agatcgtgcc gccctcgacc cggaaaacga tccggtctcc gctgttgata cgggcttcca
     3361 ggtagtgctt ctcgggatgg atcggcttga ttcggagccc gggtgaatca acctgcttga
     3421 tgagagcgat cgccgcttcg tcacaggcgg tctggcggtc ggggctgagc gcctgatagc
     3481 tcgccttcca ctcctcggac cagacggccg tcaggaagat ctgtcggcgg ggcatcttcg
     3541 gttcagcgag ccgccgtgct cttcttcagg gaatcgagaa acgcctgggc ctgctccggc
     3601 gtctcggtac gggtcgaccg gccctcggcg acgtccgcct cggcccggtt gagccgtgcc
     3661 tgcatctcgg ggtggtggaa ccagagttgg tcccgaggta tcaaagcggc ggggaccagc
     3721 tcgaacgtcc ctctgtccgt gcgctcgagg agaaccagat caccggcctt caagcccagg
     3781 gctgagcgga cctcctccgg tagagtcgct cgccctttgc tatccacggt gagaaatgac
     3841 actgtgtcac ccaagcgagc tacctccaga gtgggattgt accactttcc catttgcgga
     3901 tggctgctga tctgggggcc gttcgcggtg gcaacggctt ggcggcgagc tccgggtttg
     3961 tcgccccgga gagctcgccg ccacggcaaa gctcagtgaa atctaccgtt cgatgccgcc
     4021 gccgaagttc ggctcgcagt acgcccggca gagacacccg ccgccggccg aaaagcacct
     4081 gcggatggag gtgcagctcg cgttgcagca gatgtcggag cagtatccag ccgcgctcac
     4141 atctctcacc gatggcccca cggtgaaacc tccgaccagc attccaagcg ccagagtcaa
     4201 cttcaggatc ttcttgggca tgatgacctc ctgagaaagc ttgaaccgcc caatgcggca
     4261 aggcgccgca gtctatagac gaaagctgag gtggtcaaac ccctcgatcc cgtagaatct
     4321 gcccccgtgc gcccgctcta tctataaggt aggccgttcc ccatgaccta ccgtctgacc
     4381 gtctcctacc gcggcgcggc ctacgccggc tggcagcgcc aggacaatgc ccggaccgtg
     4441 caggaggtcc tggaggaggc gctgtcggct cttctcgggc ggaccgtccg cgtcgtcggg
     4501 gcgagccgga cggacgcggg ggtgcatgcg cgcgggcagg tggcccatct ggagctcgac
     4561 gcgccgtttc ccgagcgcgg gctcatccat ggcaccaacc accacctgcc ggaggatgtc
     4621 cgggtgctgg ccgccgcgcg ggtgcccgac ggcttccacg ccaggaagca cgccctcggc
     4681 aagacgtaca gctaccggct gagccgcgcc gccgtcctct cccccctgga cagcctgttc
     4741 gtggcccagg tgccggaccg gctcgacctc gcggcgatgc gcgaggcggc cgcccacctc
     4801 cccggccggc acgacttctc cgccttcgcc ctcgccggcg gcgcccacgg ccagccgttc
     4861 cgcaccatcc tcgccgcccg ctgggaggag atgggcgacg agctgcgctt ccggatcctc
     4921 ggcgagggct tcctgcgcgg catggttcgt gctttggtgg ggacgctgat cgaggtgggg
     4981 ttgggacgac ggacccccgg cgacttcgcc gggctcctcg acggccgtcc gcgcgccgcg
     5041 gccggcccga ccgcccccgc gcacgccctg gtgctggagc gggttttcta tccgccagaa
     5101 tggcagacgc caggcgatcc tcctgacgtt atggtacttt aagggctcca gagcgatggc
     5161 cgacctctcc aaactcgccg acccgggctc gcccgaagag ctgctcgtcc gccggatcga
     5221 catggcgcgg ctgcctcgcc atgtcgccat catcatggac ggcaacggcc gctgggccaa
     5281 ggccaagagc ctcccccgcg tcgaaggcca ccgcgccggc gtggcggcgg tgcgcgacac
     5341 cgtcgagacc gcggcgcgcc tggagctcga ggggctcacc ctctacgcct tctcggtcga
     5401 gaactggaag cgcccgcgct tcgagatctg gaccctgatg aacctcctca aggagtacgt
     5461 ccgcaaggag cgcgaggcgc tggtcgacaa cgacatccgc ttcaacgtcc tcggccgctg
     5521 ggaggagctc gacccgtccg tcgtgcgcga gctgcgcacc accctggagg cgacccgcca
     5581 ctgccagggg atgcagttca acatcgcgct caactactcg ggccgctgcg agatcgtcga
     5641 cgcctgccgc cgcatcgtgg ccgactgggc cgcgggcaag cacgccgaca tcgacgagga
     5701 gacgatcggc cgctacctct acacctcggg ccaggccgac ccggacctgc tgatccgcac
     5761 ctccggcgag atgcggatct ccaacttcct cctctggcag atcgcctaca ccgagatctg
     5821 ggtgacccgc accctctggc ccgacttccg gcgccgccac ctctatgagg ccatcctcga
     5881 tttccagggg agggagcgcc ggtacggcgg cgtcgaccag acgggcgagg cggagcagcc
     5941 ggacctcgtc gcctcggccc accgccaggc ctgatccgca cgaccccgcg atgcagcgcc
     6001 tcctcaccgc ggccgttgcc acccccctgg tcttggcggc cgtcttcctg ctgcccggcc
     6061 tctggttctt ccttttcgtc gccctcgcca tcgagctggc ggtctacgaa tacatcacca
     6121 tcgtccgcca tcgcgcgccc aacgcgccgc tccggctgct gctggtgctg gtgccgctcg
     6181 cctccctcgg catctggtgg tggctgtccg gcgaggcgtc gccggtgcag ccgggcctgc
     6241 cgctgctggt gggggcggtg ctgatgtcgg tcggcgtcgg cacgctcgtc ctcttcggca
     6301 agacgccgct cgaggagacg ctcacgacgc tcggcatctt cggcttcggc atcccctact
     6361 tcgcggtggc cgtcgccagc acggtgggga tccagcagac cgccgacccg ggcggcgtgc
     6421 cgaaccacgg cccctggctg atcgtcctgc tcttcgccat cgtctggctg ggggacaccg
     6481 ccgcctacta catcggctcg cgctgggggc gccacaagat ggcgccggtg atcagcccca
     6541 agaagagctg ggagggggcg gcggcggggt tcgtcaccgg cctgctggcg gccgtggcct
     6601 ggagctacct gcggctggga cggctggagc cacgcctcct ggtggtcgcc gcggtgaccg
     6661 cggcggcggc ccagatcggc gacctggtgg agtcgatgat caagcgcggc gccggggtca
     6721 aggactccgg gagcatcctg cccggccacg gcggcttcct cgaccggctc gacgccctgc
     6781 tcttcgccgc ccccgtcctc ctcctcgccc tctgggtcct tggcctccat ggcatgaccc
     6841 ggtgaccccg ctgactccct gacggggccg ggaatgccgg gacggcgacg gccgttcggt
     6901 ataatcggcc tcttctttaa cgaaccacgg cagccaaacg aatgaatctc ctgaccaacg
     6961 cactcgcctt catcttcgcc ctcggcatca tcattttcgt ccacgagggg ggccatctga
     7021 tgatggccaa ggccttcaag gtgagggtga tgaccttctc cctcggcttc ggcaagcgcc
     7081 tctggggatt cacccgcaac ggcaccgact accgggtgtc ggcgatcccc ttcggcggct
     7141 acgtgcggat ggggggcgag aacccggagg agtccaccgg cgaccccaac gagttcctga
     7201 gcaagccgcg ttggcagcgg atcctggtct acctggcggg cccggcgatg aacttcatcc
     7261 tcgccatcgg cctgttcgcc atcctcttca tggtgggcat cgaggtcccc aacctccccg
     7321 acacgccgcc cctgatcggc ggcgtgcagg agggctcgtc cgccgcgcag gcgggcctgc
     7381 ggcgcggcga cctcatcgtc agcatcaagg gggaggaggt cgacagctgg caggacgcgc
     7441 tgaccgagct gctgacctcg gccggccggc cggtccccct cggcatcaag cgcggcgagc
     7501 agaccttcac cgcggtcgtc accccgacca aggacccgcg ctccgagctg ggcgacctgg
     7561 cgggcctcta ccccagcttc cggccgcaga tcacccacgt ggcgcccggc ggcccggcgg
     7621 agaaggcggg gctcaagccc ggcgacgaga tccgctcgat cgacggcaac cccgtggccg
     7681 acagccgtga cttcgtgagc tacatcgaga agcgcccggg ccagcccatc cgcctgcaga
     7741 tcatgcgcga cggccggccg ctggagatcc cggtgacgcc gcagctcaac ggcaccgtgg
     7801 gccgcgtcgg catccagctc gggatcttcc agcgctacgc gccgctgcgc gcggtgcagg
     7861 agggggcgcg ctacagcctg gagctggtgc gccagacctt ccagatcatc ggcaagatct
     7921 tcaaccgcga ggtctcggcc aagggggcgc tcgccggccc gatcgagatc gcggcgcaga
     7981 gcggcgccgc ggcgcgggag gggttcaaga ccctggtcca cctgatgggc ttcctctcga
     8041 tcggcatcgc gatcatgaac ctgatgccga tccccatcct cgacggcggc cagacgctga
     8101 tcctgatggt cgagggggtg atccggcgcg acctgtcgct gcgcctcaag gaggcgatca
     8161 gccacgtcgg cttcatcctg atcctgctgc tgacggtgat ggtcctctgg ttcgacctgc
     8221 tcaagcacat gcccgagggg ttgatccccg gctcgtaggc tgcccgtggg aggggtgagc
     8281 tatcatccct cccatgcccc gtccgagctc atcggtctcg atcggcgacg aagccccgaa
     8341 cttcgacctc agctccaccg agaacgccgt cctgatgctc aaggacgagc tcgcccgcac
     8401 gtccgtcctc ctctacttct tcgccagcgc cgagagcgac cgggtacgcc gagacctgga
     8461 ggccctggag cggcggcgcg aggcgctgaa ggggttacgc ggccgcatcc tcggcgtctc
     8521 ccccctgacg gtggacgccc tccggaaggt gcaggccgat tccaggctgc gcttccccct
     8581 gctctgcgac gaccgcaact tctccgcctc ctatggagtg ggccccatcg aagagggccg
     8641 gccggccgat ccggcgctgg tcctggtgag ccgcaagcag aagatcctct tcgtcgccaa
     8701 ccccgtggcc gccgtggaag aggccctgcc ccaggtcgag aagctcctca aggatctccc
     8761 gtccccgacg agggattacc cgaagtcggt catcaaccgc tggatcgacc gctgggtgaa
     8821 ctgaggggta tagggggacg ttcccctcac tgaggtgaat cgatgagcaa cgtagagatg
     8881 ctcgaggcgc agatccagaa gctttccccg acagagctcg ccgagtttcg cgactggttc
     8941 gcggcttacg acgccgaggc ctgggaccga cagttcgaaa aggacgtcga ggccgggaag
     9001 ctggatgcat tggccgagca agctctccgg cagcgtgccg ccgggctgtg cacggacctt
     9061 tgagacatca ggcctcgccg tcccccgcga aaagaccgcc atacccgagg ggatgagccc
     9121 ctacccaggg aggtgaaggc tatccctccg gcgatgaagg ccgtacccgg tcgatgaggt
     9181 ccgtacccgg ggagatggag tgcatacccc gggtaggaag gccgtgccgg tggggatggt
     9241 ggccgtaccc cgggtatgga gcccgtaccc gcggggatgc cggtcgttac cagaccagag
     9301 ccgtcaccag agcttccgga gccgttccct tcagccataa gccgctcccg ttcagccaga
     9361 aacctgggtg tcctgactca atgtgggtgg tggaggaggt ctcccccctt tcggggggag
     9421 gggacgaagc gttctctagc aggaccgggt gggacaggtg taagtgcccc agcattcata
     9481 ccatcgggtc ctacccaggc agtcgtcgaa gttggtgatc acccccacgc ccacggcggg
     9541 ctcttctagc gtcgctccag cgagcgcacc aagctgggcg ggctcgagtc ggcgaagcgt
     9601 ctccttcgcc agcaccagct tcttcgcagt tttcttcata gtcactccgt agttagggta
     9661 cgcgccaccg ggcgcgaggg cattgtaccg ggtccgcgcc tccagccagg cgagacaccc
     9721 caagaggcga gccgctcaac gccgtggcct gctcccggaa cggcagagca ggccacggat
     9781 cggtagctag gactacaggg actgctcagc agcggcggtc cgctcgtcga ggatcgcccc
     9841 gctcctctcc agggtgaccg cgccccccgc ctcgaggaag taggccaggg aatccggcga
     9901 ctggagaaaa gccccctcct tgagcccagg gggggaggca ccaccgccgc ggatcagctg
     9961 cgcttggaga aggccttctt cctccgtcgc cgcttcagcg ccggtgctcc ggccagactc
    10021 cgcaaggtat tcaggtcgcg acggtggcct ttcatgtcgt cgccgggctc ggtctccagg
    10081 accatgggga tggcggcgag gcgcggctcg gtcaccagcc aggcgaagag gtcgacgccg
    10141 atctccccgt cgccgatgtg ggcgtgacgg tc
//
LOCUS       DASPJI010000737         8464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_217298, whole genome shotgun sequence.
ACCESSION   DASPJI010000737 DASPJI010000000
VERSION     DASPJI010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8464)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217298"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4012)
                     /locus_tag="VEL74_20700"
     CDS             complement(<1..4012)
                     /locus_tag="VEL74_20700"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0034081 - polyketide synthase complex
                     [Evidence IEA];
                     GO_process: GO:0030639 - polyketide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HYG65014.1"
                     /translation="MTDIQPQRHGAEVAVIGMACRFPGAPDADAFWDNLRNGRESIVA
                     FSEAEAAAAGVPPVLLARPDFVRAGALLDGVDLFDPALFGLTPREAEVMDPQHRLLLQ
                     CAWEALESAGYDSDRHPGLIGIWAGASAAAYLLNLLAHREVAAAVGGFQLEIGNEKDH
                     LAPQVAYRLNLRGPAVTIQTGCSTSLVAVHLAVQSLLGFECDLALAGGVSIDLPQRPG
                     YLHQEGGVASPDGHCRAFDARAAGTVFGSGVGLVVLKRLEEALADGDTVLAVIKGSAV
                     NNDGSRKVGYTAPSVAGQAEVITQALAMARLAPREISYVEAHGTGTVLGDPVEAAALV
                     RAFGPAGGPGTCALGAVKTNVGHLNVAAGLAGFIKAVQMLRHRQIPPTLHFERLNPEI
                     DFGRSPFYVETRLRDWDPPGGIRRAGVSSFSIGGTNAHVVLEEAPPVPEAPADRRSSH
                     LLVLSARTPAALESATEKLATFLESGRGADLAGVAHTLQVGRRELAHRRAVVARSVED
                     AVEALRRLPAERSATGAAPGGHRPVVFLFPGQGTRLPGAGRSLYAAEPLFRREIDRST
                     EVLASRFDLRRLLFAERYDEEAARQLSETALAQPVLFTLEHALARLWIERLGRPQIML
                     GHSLGEYVAACVAGTFAFEEALDLVAERGQLIQETPPGAMLAVPLAEEETLALLDGDA
                     ELSLAAVNEPAGCVVSGPAAAVERCEERLAARGLAGRRLPVTRAFHSALVEPAVAVLV
                     ERVARMRLSLPEIPWISNLTGRPVLPEEAVDPAYWGRHLRRPVRFADGIGEILADPDR
                     VLLEVGPGDALGRLVRRHPACRPETQVVSSLGRAEAGLQEDEALATALGRLWAAGVEV
                     EWPSGHRARRMLLPTYPFERHRYWIDGSNGADPPDLGLPEPPRDDLADPAGWFSMPTW
                     KRTPLPDPLAAQPAEDEGDWLVAGEHSGLASALAGKLRQSGREVFIAEPGPRFGRLGE
                     RAWAIQFAERADWAALLAAIAKEGGRPGRVLHLGCFAAGEGASPDGRGEVEGEGEWLE
                     RGFYSLLAFAQAIGDRGLGHPMLVGVISSGMQAVAGETALFPVKATALGPCRVIPKEY
                     PNLACRSIDVTPPPPRSWQETRLAERVLAEMGSGPADFAVAYRDQARWAMTLEPVRLE
                     SPPAGALPAKLRERGVYLITGGLEGIGLAVARDLARRVRARLVLAGRAPLPAEGVLVE
                     LEAAGAEVLAAPGIDVANRGAMAGVVERARQRFGPLHGVIHAEEPAGSGLIQFRDRAA
                     SAAVLATKVGGMRVLDELLSGLGLDFFVLFSSAFALTGGLGLVDHCAANAFLDAFAQC
                     ESPRRETLLLALDWSVW"
     gene            complement(4019..>8464)
                     /locus_tag="VEL74_20705"
     CDS             complement(4019..>8464)
                     /locus_tag="VEL74_20705"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65015.1"
                     /translation="VRSPDALLELLGREQVTVFSQTPSAFYTLVQADAAMTPTVPLVL
                     RWVIFGGEALDFQALTPWIERRGDREPRLINLYGITEITVHATWRPVTRADLAVDGSR
                     LGHPLSDLRLYLVDPYGSLVPAGVPGEICVAGEGVARGYLGHPALTAERFVPDPWSGV
                     PGARMYRSGDLARHRPGGDLEYLGRIDFQVKVRGYRIEPGEIEAALVRYPSLAAAVVV
                     ARPEGGSRRLIAYCVPASRADRPTAGELRDFAAARLPEHMVPAAFVLLDRLPLTVNGK
                     LDREALPAPGPERPNLGIAYVAPRNEVEEGLARVWREVLGLERVGVHDSFFALGGDSI
                     LSLRAHSLAAEQGLRFELPQLFTYPTIAGLARETKAGAGASSAGHVSPETAPFSLLTP
                     EDRRRLPGGLDDAFPLSTVQAGMLYHMRYSPGAIVYHNVYSHRLRCQLDPEAFLRAAQ
                     RVVDRHPILRTSFDLTSYDEPLQLVHSEATIRVGVVDLSLLSSAGQERALDDLMEAEK
                     RRRFDPARPPLVRLYLHRRDDESFQLTYTEFHPIFDGWSLHAFLAEVFAGYFALLDGR
                     SLPDEPPPRLGMRDFVSLERAAAEAPDCRAYWTRVLTGARPSILPRWSGGESPSGPAI
                     RTVVGALAAPVQEGLRRLARAAAVPLKTVLLSAHLEVLSRIGGGPGLLTGVLVNGRPE
                     GPDGDRALGLFFSVVPFRTPDVDGTWRDLVQATFAAEREMLPFRRYPLARMQQNQGGK
                     PLFDVLFNFIHFYVLRDLLESSRVALAEGSRRWEETHMPLSVTFTQDPVGETLWLELR
                     YDCRQIAPWQAEALSRTYRRTLAAMALAPEERRGLRLLLNPAEKHQILVEWSGAGSAL
                     RPQEPRGTVFGLFRAQAERAPDAIAAVCEEQILSYAGLAARAGRVARWLRRAGVGPET
                     RVGISMQRSPDLLAAMLGVLAAGGAYVPLDPAFPPERTGLLLEEAGISLVITHRRLAR
                     SVPLPQGLAVLELDTDWETQRQELAPVHDPGEGAAAYVLFTSGSTGVPKGVVVENRQL
                     VSYVRGILERLAPPAGAHWAMASTFAADLGHTTLFPSLLTGGCLHVLPEDRAANPEAW
                     EEHGLSRPVDILKIVPSHLSALLASPGAAAILPRARLVLGGEALDPGLVARVRALAPD
                     LQVFNHYGPTEATVGVLCGRVDVSHPAGAATVPLGRPLAGSRIHLLDPWLLPVPAGAP
                     GELCIAGTGLARGYLGQPGATAARFVPDPFGGERGGRLYRTGDQARWLPDGRIEFLGR
                     QDGQVKLRGFRVELREVELELARHPEVREAAAAVRESRAGDWRLVAFAVPAGAGLPTD
                     AELRSFLRRRLPDYMLPAGFVRLAELPRTANGKLDRAALPEVPDLADAWQAGRVAPRN
                     ELERAIAAIWQEVLGLDAVGIDDNLFDLGGHSLTLLRIHSKLRSSLERDLPMVALFEH
                     PTIGGLASHLGAGDEMPAAPAERVAARAEARHAALAERRRARSTR"
BASE COUNT         1299 a         3107 c         3002 g         1056 t
ORIGIN      
        1 gccagacgct ccagtcgaga gcaaggagca gggtctcgcg ccgcggcgac tcgcactggg
       61 cgaatgcgtc gagaaaggcg ttggccgcgc agtgatccac caaccccagg ccgccggtca
      121 gcgcgaaggc ggaggagaac aggacgaaga agtccagccc caagccgctc agcagctcgt
      181 cgagcacgcg catcccaccc accttggtgg ccagcacggc cgccgacgcg gcccgatcgc
      241 ggaactggat caggccgctt cccgcgggct cctcggcatg gatgacgccg tgcagcggac
      301 cgaagcgctg gcgcgcacgc tccacgactc cggccatagc gccgcggttc gccacgtcga
      361 tcccgggagc ggccaggacc tcggcccctg ccgcttcgag ctccaccagc accccctccg
      421 ccggcagcgg cgcgcgcccg gccagcacca gacgggcacg cacgcgccgg gcgaggtcgc
      481 gagccacggc taacccgatc ccctccagtc caccggtgat caggtagacg ccccgctccc
      541 gcagcttcgc cggcagtgcc ccggccggcg gcgactccaa tcgcaccggc tccagggtca
      601 tcgcccaacg cgcctggtcg cggtaggcca cggcgaaatc ggcgggcccg ctccccatct
      661 cggcgagaac ccgctccgcg agccgggtct cctgccagct ccgcggcggc ggcggcgtca
      721 cgtcgatact gcggcaggca aggttcgggt actccttcgg gatcacccgg cagggcccca
      781 gcgccgtcgc cttgacgggg aagagggccg tctcccccgc caccgcctgc atgccgctgg
      841 agatcacgcc cacgagcatc ggatggccga ggccgcggtc cccaatggcc tgggcgaaag
      901 ccaacaggct gtagaaaccc ctctccagcc actcgccttc tccctccacc tccccccgac
      961 cgtccggtga tgccccctcg cccgcggcga agcagcccag atgtaggacc ctgccgggtc
     1021 tccccccctc ctttgcgatg gccgcgagca gagccgccca gtccgcccgc tcggcgaatt
     1081 ggatggccca ggcccgctct ccgagacggc cgaaacgggg cccgggctcg gcgatgaaga
     1141 cctcgcggcc gctctgtcgg agcttgccag ccagcgccga tgccagaccc gagtgctccc
     1201 ccgccaccag ccaatctcct tcgtcctccg cgggctgcgc ggcgagcgga tcagggagcg
     1261 gagtgcgctt ccaggtgggc atggagaacc agcccgccgg atcggcgagg tcgtcccgcg
     1321 gcggctccgg caatccgagg tccgggggat cggcgccgtt gctgccatcg atccagtagc
     1381 gatggcgctc gaagggataa gtggggagca gcatgcggcg tgcccggtgg ccggacggcc
     1441 actccacctc gacgccggcg gcccagaggc gcccgagggc cgtggccagg gcctcgtcct
     1501 cctgcaggcc ggcctccgct cgccccagcg aggagaccac ctgggtctcg ggtcggcatg
     1561 ccggatgccg ccggaccagc cgccccaggg catcgccggg cccgacctcg aggaggaccc
     1621 ggtcgggatc ggccaggatc tcgccgattc cgtcggcgaa gcgcaccggc cggcgcaggt
     1681 gccggcccca gtaggcggga tccaccgcct cctccggcag gaccggccgg ccagtcaggt
     1741 tggagatcca cgggatctcc ggcaaagaca gtcgcatcct cgcaacccgc tccactagga
     1801 ccgcaacggc gggctccacc agagccgaat ggaaggcccg cgtcaccggc agacgccggc
     1861 cggcgagccc ccgggccgcc agccgctcct cgcagcgctc caccgccgcg gcagggccgg
     1921 ataccacaca gccggcgggt tcgttgaccg cggcaagaga gagctccgca tcgccgtcca
     1981 ggagagccag ggtctcctcc tccgccagcg gcacggccag catcgccccc ggcggcgtct
     2041 cctggatcag ctgtccccgc tcggccacca ggtccagcgc ttcctcgaag gcgaaggtgc
     2101 cggcgacgca cgccgccaca tactcgccca ggctgtgtcc gagcatgatc tgtggccgtc
     2161 ccagccgctc tatccagaga cgcgccaagg cgtgctccag ggtgaagagg acgggctggg
     2221 ccaacgccgt ctcggaaagc tgccgggccg cctcttcgtc atacctctcg gcaaagagca
     2281 gccgccgcag gtcgaagcgg gacgccagca cctcggtgct gcggtcgatc tcgcgccgga
     2341 agagcggctc ggcggcgtag agcgaacgcc cggctccggg gagccgcgtc ccctggccgg
     2401 gaaagaggaa gacgacgggc cggtggccgc cgggcgcggc gccggtggcg ctccgctcgg
     2461 cggggagccg ccgcagggct tcgacggcgt cctccacgct gcgggccacc acggcgcgcc
     2521 ggtgggcgag ctcccgccgc cccacctgga gcgtgtgagc gacgccggcc aggtccgcgc
     2581 cgcgcccaga ctccagaaag gttgcgagct tttcggtggc gctctccagg gcggcagggg
     2641 tgcgcgccga cagcaccagc agatggctgg accggcgatc tgccggcgcc tccggcacgg
     2701 gcggcgcctc ttcgaggacg acgtgggcgt tggtgccgcc gatgctgaac gagctcacgc
     2761 cggcgcggcg gatgcccccc ggcgggtccc agtcccgcag ccgcgtctcg acatagaacg
     2821 gactgcggcc gaagtcgatc tccgggttga gccgctcgaa gtgaagggtg ggagggatct
     2881 ggcgatgccg cagcatctgg accgctttga tgaagccggc caggccggcg gcgacgttga
     2941 ggtgcccgac attggtcttc accgccccca gcgcgcaggt ccccggcccg ccggcggggc
     3001 cgaaggcgcg caccagggca gcggcctcga cggggtcgcc cagcaccgtc cccgtaccat
     3061 gggcctcgac atacgagatc tcccgcggcg cgaggcgggc catggcgagc gcctgagtga
     3121 tcacctcggc ctgaccggcg acgctggggg ccgtatagcc gaccttgcgg gagccgtcgt
     3181 tgttcacggc cgagcccttg atcaccgcta gcaccgtatc gccgtccgcc agtgcctcct
     3241 ccagacgctt gagcaccacc agccccacgc cgctgccgaa gaccgtgccc gccgcccgtg
     3301 cgtcgaaggc acggcagtgg ccgtcgggcg acgcgactcc cccctcctgg tggagatagc
     3361 ccggtcgctg cgggagatcg atggagaccc cgccggcgag ggccagatcg cactcgaagc
     3421 cgagcaggct ctggactgcg agatggaccg ccaccagtga cgtggagcag ccggtctgga
     3481 tggtgaccgc ggggccgcgc aggttcagcc ggtaggcgac ctgcggcgcc aggtggtcct
     3541 tctcgttgcc gatctcgagc tggaaaccgc ccactgcggc cgcgacttcg cggtgggcca
     3601 ggaggttgag gaggtaggcg gcggcgctcg cgccggccca gatgccgatc aggccgggat
     3661 ggcggtcgga atcgtagccg gcgctctcca gcgcctccca ggcgcactgc agcagcagcc
     3721 ggtgctgcgg gtccatcacc tcggcctcgc gcggcgtcag gccgaagagc gccggatcga
     3781 aaaggtcgac tccgtcgagc agggcgccgg cgcgcacgaa gtcgggcctg gccaggagca
     3841 ccggcggcac cccggccgcc gcggcctcgg cttcggagaa ggcgacgatc gactcgcggc
     3901 cgttccgcag gttgtcccag aacgcgtcgg cgtccggcgc gccggggaaa cggcaggcca
     3961 ttccgatgac ggccacctcg gcgccgtggc gttgcggctg gatgtcggtc attgctttct
     4021 acctcgttga tcttgcccgg cgcctctccg ccagagccgc gtgccgggcc tccgcccggg
     4081 cggccacccg ctcagcgggt gccgcgggca tctcgtcgcc ggcgcccaga tgggaggcca
     4141 ggccaccgat ggtcgggtgt tcgaacagcg ccaccatcgg cagatcccgc tccagggagc
     4201 tccgcagttt gctgtggatc ctcaacaggg tcagggagtg ccccccgagg tcgaagaggt
     4261 tgtcgtcgat gcccaccgcg tcgagcccca gcacctcctg ccagatcgcg gcgatggcgc
     4321 gctccagctc gttgcgcggc gcgacgcggc cggcctgcca agcgtcggcg aggtccggca
     4381 cctcggggag ggcggcgcgg tccagcttgc cattggcggt ccgcggcagt tccgcgagcc
     4441 gcacgaaacc ggccgggagc atgtaatcgg gcagccgccg gcggaggaaa gaccgcagct
     4501 cggcgtccgt gggcaggccg gcaccggccg gtaccgcgaa cgccaccagc cgccagtccc
     4561 ccgcccggct ctcgcgcacc gccgccgcag cctcccgcac ctcgggatgg cgggcgagct
     4621 ccagctccac ctcgcgcagc tcgacgcgga agccgcggag cttgacctgg ccgtcctgac
     4681 gccccaggaa ctcgatccgg ccgtccggaa gccagcgggc ctggtcccct gtgcggtaga
     4741 ggcgtcctcc ccgctcgccg ccgaacgggt cggggacgaa ccgcgcggca gtggcgccgg
     4801 gctggcccag gtagccgcgc gccaggccgg tgccggcgat gcacaactcg ccgggggcac
     4861 cggccggcac cggtaaaagc cacgggtcca ggagatggat gcgggagccg gcgagcggcc
     4921 gtcccagggg gacggtggca gcgccggctg ggtgcgagac gtccacccgg ccgcacagga
     4981 cgcccacggt ggcctccgta gggccgtagt gattgaacac ctggaggtcg ggggcgaggg
     5041 cacgcacccg tgcgacgagg cccgggtcca gcgcctcgcc gcccaggacc aggcgggcgc
     5101 gcggcaggat cgccgccgca ccgggggaag cgaggagcgc cgagaggtgg gaagggacga
     5161 tcttgaggat gtccaccggc cggctcagcc cgtgctcctc ccaggcctcc gggttcgcgg
     5221 cgcgatcttc cggcaggacg tgcaggcagc cgccggtcag cagcgaaggg aacaacgtgg
     5281 tgtggcccag gtccgccgcg aaggtcgagg ccatggccca atgcgccccg gccggcggcg
     5341 ccagccgctc caggatgccg cgcacgtagc tcacgagttg ccggttctcc accaccaccc
     5401 ccttggggac gccggtcgag cccgaggtga agaggacgta agcggcagcg ccctcgcccg
     5461 gatcgtgcac cggggccaac tcctgccgct gcgtctccca gtccgtgtcg agctccaaga
     5521 ccgccagtcc ctgagggaga ggtacggacc gcgctagccg ccggtgggtg atcaccagcg
     5581 atatccccgc ctcctccagg agcaggccgg tcctctccgg ggggaaggcg gggtccaggg
     5641 gtacgtaggc tccacccgcc gcgagtactc ccagcatggc ggccagcagg tccggggagc
     5701 gttgcatgga aataccgacc cgcgtttccg gccccactcc ggcccggcgc agccagcggg
     5761 cgacacggcc cgcccgggcg gcgagcccgg catagctcag gatctgctct tcgcagaccg
     5821 cggcgatggc gtcaggagct ctctccgcct gagcccggaa cagcccgaag acggtacccc
     5881 gcggctcctg cggccggagc gcgcttcccg cgccgctcca ctcgaccagg atctgatgct
     5941 tctcggccgg attgaggagg agccggagcc ctcgccgttc ctcgggagcc agagccatgg
     6001 cggcaagggt ccgccggtag gttcggctca gagcctcggc ttgccagggc gcgatctgcc
     6061 ggcagtcgta gcgcagctcg agccagagcg tctcccccac cgggtcctgg gtaaaggtga
     6121 cgctcagcgg catatgggtc tcttcccagc gcctggagcc ttccgcgagc gctacccggc
     6181 tcgactccag gaggtcgcgc aggacgtaga agtggatgaa gttgaacagc acgtcgaaca
     6241 gcggcttgcc gccctggttc tgttgcatgc gggccagagg gtagcggcgg aacggcaaca
     6301 tctcgcgctc agcggcgaag gtggcctgca ccaagtctct ccacgtcccg tccacgtccg
     6361 gagtgcggaa cgggacgacg ctgaagaaga ggcccagggc gcgatcaccg tctggcccct
     6421 cgggccggcc gttcaccagc accccggtga gcaggcctgg cccaccgccg atccggctca
     6481 ggacctccag gtgggcactg agcagcacgg tcttgagcgg caccgccgcg gcgcgggcga
     6541 ggcgccgcaa tccctcctgg accggcgccg ccagggcacc gaccaccgtg cggatcgcag
     6601 gccccgatgg gctctccccg ccgctccagc gcggcagtat cgagggccgc gctcccgtga
     6661 gcacccgcgt ccagtaggcg cggcagtccg gtgcctcggc cgcagcccgc tccaggctga
     6721 cgaagtcgcg catgccgagg cgcggcggcg gctcgtccgg cagcgaccgg ccgtcgagca
     6781 gggcgaagta gccggcgaag acctcggcca ggaaggcgtg caggctccaa ccatcgaaga
     6841 tggggtggaa ctcggtgtag gtgagctgga agctctcgtc atcgcgccgg tggaggtaga
     6901 gacgcaccag gggcggtcgc gccgggtcga agcggcggcg cttctcggcc tccatgaggt
     6961 cgtccagggc ccgctcctgc ccggccgacg agaggaggct caggtccacc acccccactc
     7021 ggatcgtggc ctcggaatgg acgagttgca ggggctcgtc ataggaggtc aggtcgaagg
     7081 atgtccgcag gatagggtgg cggtccacga cacgctgcgc cgcccgcagg aacgcctccg
     7141 gatcgagctg gcaacgcaac cggtggctgt agacgttgtg gtagacgatg gcgcccggcg
     7201 agtagcgcat gtgatagagc attcccgcct ggaccgtgct cagcgggaag gcgtcgtcca
     7261 gcccgcccgg cagacgccgg cggtcctccg gagtcaacaa ggagaaaggc gccgtctccg
     7321 gcgagacgtg accggcagag cttgcccccg cgccagcctt cgtctcccgt gccagcccgg
     7381 cgatggtcgg ataggtgaag agctggggca gctcgaaacg cagcccctgc tccgcggcga
     7441 gggagtgggc ccgcaggctc aggatcgagt ccccgcccag ggcgaagaag ctgtcgtgca
     7501 cgccgacccg ctcaagcccc agcacctctc tccagaccct ggccagcccc tcctccacct
     7561 cgttgcgcgg ggcgacatag gcgatcccca ggttcggacg ctcgggacca ggggccggga
     7621 gagcctcgcg atcgagcttg ccgttgacgg tgagcggcag cctgtcaagg aggacgaagg
     7681 cggccggcac catgtgctcc gggagccgcg cggcggcgaa gtcgcgcagc tcaccagccg
     7741 tcggcctgtc cgctctggac gccgggacac agtaggcgat cagccgccgg ctcccgcctt
     7801 ctggccgtgc cacgaccacc gccgcagcca gggatgggta gcggaccaag gccgcctcga
     7861 tctcccccgg ctcgatgcgg taaccccgca ccttcacctg gaagtcgatc cgcccgaggt
     7921 actcgaggtc gccgcccggg cgatggcgcg ccaggtcgcc actgcggtac atccgcgccc
     7981 ccggaacgcc gctccatgga tcgggcacga agcgctcggc ggtcagggcc ggatggccta
     8041 gatagccgcg cgccaccccc tcccccgcca cgcagatctc gcccggcact cccgcgggaa
     8101 ccaggctacc gtaagggtcc accaggtaca gccgcagatc ggagagcgga tgtccgaggc
     8161 ggctgccgtc caccgccaga tccgcccggg tcacgggccg ccacgtggcg tgcaccgtga
     8221 tctcggtgat gccgtaaaga ttgatgagcc gcggctcgcg gtcgcctcgg cgctcgatcc
     8281 agggagtgag agcctggaaa tccagggcct cgccgccgaa gatcacccag cgcaacacca
     8341 gggggacggt gggcgtcatg gcagcgtccg cctggacgag ggtatagaaa gcggagggag
     8401 tctggctgaa aaccgtcacc tgctcgcggc cgagcagctc cagcagcgcg tcgggcgagc
     8461 ggac
//
LOCUS       DASPJI010000738         8746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_104375, whole genome shotgun sequence.
ACCESSION   DASPJI010000738 DASPJI010000000
VERSION     DASPJI010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8746)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8746
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104375"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VEL74_20710"
     CDS             <1..512
                     /locus_tag="VEL74_20710"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG65016.1"
                     /translation="QAEPRLWNIAHLNLATDYCFLGRYEEASRLMNEVRPLVTAQGDA
                     LDLIRILGLEGRIAAGLGQYREARRFFQQARREFTARKMWYDVALALLEEAIVLLDEG
                     RTADVKEIATDLQVVFDANGVHREALAALRLFAEAAKREAATAEMGRRVLRYLFRARH
                     DEELKLTEA"
     gene            complement(987..2192)
                     /locus_tag="VEL74_20715"
     CDS             complement(987..2192)
                     /locus_tag="VEL74_20715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65017.1"
                     /translation="MGRSALLVFLLACLSWADPATAGLQPEPFASGELSHWDGPQFVE
                     ADRAGHVYFLRADTLQVYPVTKEGTLGEPIRLKPGAKPIGEILNAAMSPDGEKWLLQS
                     PFSIWLFADGKEKPVPPLTWKPESVGFHRDDPLVAVAPMPLGKRIDPDKIATPPWLLE
                     LGSDRWSVLRDLEGVSVAELLASGEKWNDAIAESSVLVAPGRLGHLWMAHQYAYRLQR
                     LSPTGRSRLEITVGDGKVQHEGEKGPSQWSHGSGALKQKGSYQTWTAATAILDLAEGP
                     DGRLYLLVPTESGGLALDRYDPVRLALERVSVKLSTEGRFTIAAGKDALYLAAWNGTK
                     GRWRLPWEALEEAEWAPVENAAMDGIPMDAEGDSSKQTSKPADRKRPAAKPAVRASGS
                     AARKVPTQE"
     gene            3866..4687
                     /locus_tag="VEL74_20720"
     CDS             3866..4687
                     /locus_tag="VEL74_20720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65018.1"
                     /translation="MLRLPDIPPQPLLLRLVLLGASNLQSSFPRVVHQARRVAGGPVE
                     VVGAFGHGRSYGAWSRFLFVRQLPGILQCGLWEELERRPPLPTVALVTDVGNDLAYGE
                     RPETIAEWVETCLDRLARLGAETVLTLLPIESLAFLSPLRYYLARTILFPGRGVPRSA
                     LLERANELNSRLQRLGLERGVRLVEPEVSWYGIDPVHIRRGERDRAWGHIVSHWGLES
                     LAGRTVRSMGEEHLPLQGRRALWGVRAAELRLFGRTIHTPQPAGRFEEGTTIALY"
     gene            4674..5822
                     /locus_tag="VEL74_20725"
     CDS             4674..5822
                     /locus_tag="VEL74_20725"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HYG65019.1"
                     /translation="MRCIRRHPFRSAGALLALSLLLTLGISAEARFLLQAGWTEARIL
                     LGRQPIHELIADPATSPERQAQLELVLAARSFAARQLGLRAGNTYTTFSDVGEGPLLH
                     VLSASRQDRLEAYRWTYPVVGSIPYKGFFDEQAALAEKRRLERAGYDTYLRQASAFST
                     LGWFSDPLLSTALGDPADLASTVIHEISHNTLWVPSAARFNESYANFVGLRGAEAFFL
                     SRGDRQAADRCAALWRDEKRLGAFYTELAERLESLYAEDLPARERERRRADLFARARA
                     VMMGPLDRSLEVYSGKAMSRRGLNNAVVVAQRIYRTGLEDFDQIHAASGEDLRSSVSE
                     IRRTVHLARLSRVDDPFRALAAKVQLVSRSGKGGGRLPAGGAVPARKG"
     gene            complement(5834..>8746)
                     /locus_tag="VEL74_20730"
     CDS             complement(5834..>8746)
                     /locus_tag="VEL74_20730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65020.1"
                     /translation="EDWVLLTSTHHIATDGWSGTIFFEEWIRCYAAILAGEAPRLPEL
                     PVQYADFAVWQRRWLEGEVLERELAYWRERLAGAPQALELPLDRPRPAVRSGAGGTLP
                     AVIPAALTAALSELSRREGATLFMTLTSAFQALLGRWAGQDDVLIGTPVAGRNRREIE
                     GLIGFFVNTLVLRAELAGSPPFHELLGRARTTTLDAFAHQALPFERLVEAVAPERDLT
                     RSPLFQVMFAFQNVPQGGTGIPGLKLSPLPVDLEVAKFELMLTLAESAGGLAGTLEYD
                     ADLFDRSTAARLLSHLGRFLEAVASDPACRIGEVPLLDPAERHQLLVEWNDSGEDHAG
                     GMCLHELVGAQVERSPDAVAVSFEGEELSYAELERRANRLAWRLRELGVGPEVPVAMC
                     VERSPAMVVGLLAVLKAGGAYVPLDPDYPAERLAYMIEDSGASLLLAQSHLVLRLPAG
                     DTRVLLLDGEEADSAFAPGAPEERPLGGAVPDSLLYIIYTSGSTGRPKGVMVRHRGVV
                     NRLLWAERAYPVTAADRVLQKAPFSFDFSVWECFGPLLAGARLVLARPGGQREGRYLA
                     RTVLEQGITLLHFVPAMLQVFLEEEGLESCHSLRFVFSGGDALSSEVRDRFRARLPEG
                     VLLRNQYGPTEISIDMTEWVCDAGDETGEGRRQPPIGRPIGNGEAYVVDAELAPVPVG
                     VAGELLLGGEGVARGYRGRPDLTAERFIPSARHGAAGARLYRTGDRARWRPDGNLEFL
                     GRVDQQVKIRGYRIEPGEIESVLLAHPAVREVAVAVRERGDRHLVACVVPAGEADLAP
                     DELRSFARERLPEYMVPARFLVLEALPLTPSGKVDRRALAALELSAEHQARTLVLPRT
                     PVEETLAAIWKELLGVEEIGVEDSFFDLGGHSLLATRLFSRVQHAFGVDLSLRQIFHA
                     PTIAALAQAVEEQLPPPEPTAVDLDRLLDQLEMLSDEEVAARLAELGGLDGGTPGG"
BASE COUNT         1389 a         3117 c         2866 g         1374 t
ORIGIN      
        1 gccaagccga gccgcgcctc tggaacatcg cccatctgaa tctcgccacg gactattgct
       61 ttctcggcag gtatgaagag gcgagccgat tgatgaacga agtgcggccc ctggtgaccg
      121 cgcagggaga tgccctcgat ctgattcgca tcctggggct ggagggacgc atcgccgccg
      181 gcctcggtca gtaccgtgag gcccggcggt ttttccagca agcccgccgg gaattcaccg
      241 cccgcaagat gtggtacgac gtggctctgg cgctgctgga ggaggcgatc gtgcttctcg
      301 acgagggccg gacggccgac gtgaaggaga tcgctacgga cctccaggtg gtgttcgacg
      361 ccaacggcgt ccaccgggag gccctggccg ccctgcggct cttcgctgag gcggcgaagc
      421 gtgaagcggc cacggccgag atgggacgga gggtgctgcg ctacctcttc cgggcgcggc
      481 acgatgagga gctgaagcta acggaggcgt gagagggcgg acgggaccga gacaacggaa
      541 cgccatgaat caccaaagag tgagagcggc cccacatcca ccctaagccc tttggtccgc
      601 gtcaggcagg gatgcgcttc ttcgggggaa cgtgggaccg ggcccggtcc tggcgccata
      661 tggcctttct catggggcat tggcagctcg gcggagcggg gatgtgagtg gtcgagcgca
      721 aggagaccgg cgccttcgtg ggagtgcggt cagttcgccc cgccgcttct tcagccggcc
      781 cggccaagcg gtcattgcgc gccacttcgc cgtcaatcgg cgccgcggag tggacagttc
      841 gccccgtgga gtggtcggtt ggcccggctt cgccgccggt gcgccacgcg gggccagcgc
      901 gccattgact ctctgtggcg ggcgcggccg gcagggcggg tgaacagttg aggtgaagga
      961 cgctctccga caacgcgccc gggcgcttac tcctgcgtag gaactttccg cgcagccgag
     1021 ccagaggcgc gaacggcggg cttcgcggcg ggcctcttcc ggtcagcagg tttcgaggtc
     1081 tgcttcgagc tgtcgccttc cgcatccatc ggaatgccgt ccatcgcggc attctcaaca
     1141 ggagcccatt cggcctcctc gagggcttcc cagggcaacc gccaacgacc tttggtccca
     1201 ttccacgctg ccaggtagag cgcatccttg cctgccgcaa tagtgaagcg cccctccgtc
     1261 gagagcttca cggaaactct ttccagagcc agccggacag ggtcgtaccg atcgagggcg
     1321 aggccaccgc tttccgtcgg aaccagaagg tacaaccgtc cgtcgggccc ttcggcgaga
     1381 tccaggatcg cggttgcagc cgtccaggtc tgataggagc ccttctgctt cagggctccc
     1441 gagccatgac tccattgcga gggccccttt tcaccttcat gctggacctt gccatcaccg
     1501 accgtgatct cgagccggga acggccggtc gggctgagcc tctggagacg atacgcgtac
     1561 tgatgcgcca tccagaggtg gcccagccgg ccgggggcca ccaacaccga gctctcggcg
     1621 atggcatcgt tccatttttc tccggaggcc agcaactcgg cgacggacac gccttcgagg
     1681 tcgcggagta cgctccagcg gtcactcccc aattcgagaa gccatggcgg tgtagcgatc
     1741 ttgtcaggat cgattctctt tcctaagggc atgggggcca cggccaccag aggatcgtcc
     1801 cgatggaagc ctacggattc aggtttccag gttagcggtg ggacgggctt ctcttttccg
     1861 tctgcgaaga gccagatgga aaatgggctc tgcaacagcc atttctctcc gtcagggctc
     1921 atggcagcat tgaggatctc tccgatgggc ttcgctccag gcttcagacg gattggctct
     1981 cccagagtgc cttctttcgt caccgggtag acctgaaggg tgtcggcccg caggaagtag
     2041 acatgtccag ctcgatcggc ttcaacgaac tgtgggccgt cccaatggga gagctcgccg
     2101 ctggcgaacg gttcaggctg aaggccggcg gtggcagggt ccgcccagga gagacaagca
     2161 agcaagaaga cgagcagagc tgagcggccc atgagacacc tcactgagga catttcctga
     2221 tatagccagt gggcttaccg tcggaagagt agaagaagca atacgtacaa ccagcaatgt
     2281 cgctgcattc ataggctgct tccgccggca gcgagctcca aatgaggccg gccaaggtta
     2341 aagtaaccag aagcaacctt ctgacagcag atgagatgcg catacgccct cccattcaag
     2401 tatataggat cgggttcttg taacagaatc caactccact tgcaagcctt gccccaaaca
     2461 gcggcggagc acctcgtcta cgggatcgac cgggaaagct acacggcctt gcaggctccg
     2521 gtggtgtccg ctggctgcga gaccccgtag gtcccagccc catttcgaaa cccgctcccc
     2581 ccttccagct tcgcatcccg gaccgcagct tacacatctt ccctttccta tctagatgca
     2641 gcttgccata ttatactaca cttccacctc gacttccgca tccgaccgga ttcggatcag
     2701 gtgtctctcc actcggtgat ctctgcctgg gtaggacaac ccggcatgcc gtcagccatg
     2761 gaacatccag ccagcagagg cggagggaac ggccctccgc ctcccggctg acctcctgtc
     2821 ggacggagga atcctcaggg gcgcggcctt ggacacggcg gcgccccgtc acaattcagt
     2881 tgtcaatgaa ccgggagccg ccgctccagg cccggtgagg gcccgaaacg aggcgctcct
     2941 tcttcagccc ggctcgccgg gaaggcgttc cgggccgttt cctcagtgct acggcgccgt
     3001 ccctcactac tacggcgccg ctcctcaatc cttcgccgcg actcctcaac actacggagt
     3061 cagtctggag cgttgagggc cgactcctca gtactacggg ggcgtccctc aatgcttcgc
     3121 cgccgctcct cagtgctacg gagccacttc ggagtgttcc gagccggctc ctcactaccg
     3181 agggatgact ccagagtact tcgggacggc tcctcaaggc tccgccgccg cgcctcaatg
     3241 cttcggagtc actccggagt gctccaggcc ggctcctcag tgctacgggg ccgtccctca
     3301 gtgcttcgct gccgctcctc agtgctacgg aatcccttgg gagtgctccg gaacggcggc
     3361 gaagcgttga gggacgacgg cagccatggc cgcggcgagg cagaatctac aaagaggatc
     3421 acctgaatgt caaggaagtc gtcataccag cattttgtgc tagtctaccc gccatgcagg
     3481 tgagcccctg gctctgacca agccgaagtg ctctcgctct ttgacaattg atgtgcacgg
     3541 ctcgatcgtg gcgcccggga gggtggggct gcgatcggac gtgcttttca ggaggtcagc
     3601 ctgggagagc cctactccct gtccgttgtg ggtgtggctc cggagccctg agtcgaaccg
     3661 cgggagatac gactcgtggc tccggagggc tggaatcccc tcccgagatt caactcgtcc
     3721 cccggtggtt tcgactcctg accggcatcg gccgaagcaa aagaggcggg cgccgaatcg
     3781 aacctggcgg agttcgattc ggctacctct cgatacgaat cagaggggtg ggagcgccga
     3841 gtcgcggtac gatgcgaacg acccgatgct ccggcttccc gatatccccc ctcagcccct
     3901 gctgctccgc ctcgtcctcc tgggcgcgag caacctccag tcctcgttcc cccgcgtcgt
     3961 ccaccaggcg cgccgcgtcg ccggaggacc cgtggaggtc gtgggcgcct tcgggcatgg
     4021 ccgctcctac ggcgcctgga gccgcttcct gttcgtgcgc cagttgccgg ggatcctcca
     4081 gtgcgggctc tgggaggagt tggagcgccg gccgccgctg cccacggtgg ccctggtgac
     4141 cgacgtgggc aacgacctgg cctatggtga acggcccgag accatcgcgg agtgggtgga
     4201 gacctgcctg gaccggctgg cgcgcctcgg cgccgagacc gtcctcaccc tgctgcctat
     4261 agaaagtctc gcattcctct ctcctctgcg ttactatctg gcacgtacga tcctttttcc
     4321 tggccggggg gttccaagaa gcgcgctcct ggagcgtgcc aacgagttga acagccgtct
     4381 ccagcgcctc ggcctggaac gcggcgtccg gctcgtggag cccgaggtgt cctggtatgg
     4441 gatcgacccc gtgcacatcc ggcggggcga gcgggaccgg gcgtgggggc acatcgtctc
     4501 ccactggggc ctcgaatccc ttgccggccg gacggttcgg agcatggggg aggagcatct
     4561 cccgcttcag gggcggcgcg ctctctgggg ggtccgtgcc gcggagcttc gcctgttcgg
     4621 gaggacgatt cataccccgc agcccgcagg gcggttcgaa gaaggtacga ccattgcgct
     4681 gtattagaag acaccccttc cgctccgcag gcgccctcct cgccctctcc ctcctcctga
     4741 cgcttgggat cagcgccgag gcccgcttcc tcctgcaagc cggctggacc gaagcgcgca
     4801 tcctgctcgg acggcagccg atccacgagc tcatcgccga tcccgcgacg tcgccggagc
     4861 ggcaggcgca gctcgagctg gtcctggcgg cccgctcctt cgccgcccgt caactgggcc
     4921 tgcgggccgg taacacctac accaccttct ccgacgtcgg cgaggggccg ctgctccacg
     4981 tgctgtcggc gagccggcag gaccggctgg aggcctaccg gtggacctat ccggtggtgg
     5041 gctccatccc gtacaagggg ttcttcgacg agcaggcggc gctggcggag aagcgccgtc
     5101 tcgagcgcgc ggggtacgac acctacctgc ggcaggcctc ggccttctcc accctgggct
     5161 ggttcagcga tcccctgctc tccaccgccc tcggcgaccc ggcggacctg gcctcgacgg
     5221 tgatccacga gatctcccac aacaccctat gggtgccgag cgccgcccgc ttcaacgaga
     5281 gctacgccaa cttcgtcggc ctgcgcggcg ccgaggcctt cttcctcagc cgcggcgacc
     5341 ggcaggcggc cgaccgctgc gccgccctct ggcgcgacga gaagcgcctg ggcgccttct
     5401 ataccgagct cgccgagcgc ctggagtcgc tgtacgccga ggacctgccg gcgcgggagc
     5461 gggagcgccg ccgggccgac ctcttcgccc gtgcccgcgc cgtcatgatg gggccgctgg
     5521 accgcagcct cgaggtctac tccggcaagg cgatgagccg ccgcggcctc aacaacgccg
     5581 tggtggtggc ccagcgcatc taccgcacgg ggctcgagga cttcgaccag atccatgccg
     5641 cctcggggga ggacctgcgg tcgagcgtgt cggagatccg ccgcaccgtc cacctcgccc
     5701 ggctgtcccg ggtggacgac ccgttccgcg ccctggccgc gaaggtccaa ctggtcagcc
     5761 ggtcgggcaa ggggggcgga cgcctccccg ccggcggcgc cgtcccggcc cgcaagggct
     5821 gaccggcccc tcctcagccg cccggggtcc ccccgtcgag gccgcccagc tcggcgagcc
     5881 gggcggcgac ctcctcgtcc gacagcatct cgagctggtc gagcagccgg tcgaggtcga
     5941 ccgccgtcgg ctcgggaggc gggagctgct cctcgaccgc ctgggcgagg gccgcgatgg
     6001 tcggcgcgtg gaagatctgc cgcagcgaca ggtcgacgcc gaacgcgtgc tgcacccggg
     6061 agaagaggcg ggtggccagc agcgagtggc cgcccaggtc gaagaagctg tcctcgacac
     6121 cgatctcctc caccccgagc agctccttcc agatcgcggc cagggtctcc tccaccggcg
     6181 tccgcgggag gacgagagtc cgcgcctggt gctcggccga gagctccagc gccgccaggg
     6241 cgcggcggtc caccttgccg ctgggcgtca gcggcagcgc ctccagcacc aggaacctcg
     6301 ccgggaccat gtactcgggc aggcgctccc gcgcgaacga ccgcagctcg tcgggcgcca
     6361 ggtccgcctc gccggccggt acgacgcagg cgacgaggtg acggtcgccg cgttcgcgga
     6421 cggccaccgc cacctcccgc accgccggat gggccaggag gaccgattcg atctcgcccg
     6481 gctcgatgcg gtagccgcgg atcttgacct gctggtcgac gcgccccagg aactccaggt
     6541 tgccgtccgg ccgccaccgc gcgcggtccc ccgtccggta gagccgcgcg ccggcggctc
     6601 catgccgcgc ggaggggatg aaccgctccg ccgtcaggtc gggacggccg cggtagcccc
     6661 gggccacccc ttcgccgccc agcagcagct ctccggcaac tccaactggt accggcgcca
     6721 gctccgcgtc cacgacatag gcctcgccgt tgccgatcgg ccggccgatg ggcggctggc
     6781 gccgtccctc acccgtctcg tcgccggcgt cgcagaccca ttccgtcatg tcgatggaga
     6841 tctccgtcgg gccgtactgg ttgcgcagca ggaccccctc cggcaggcgg gcgcggaacc
     6901 ggtcccgcac ctcggacgac agggcgtcgc cgcccgagaa gacgaagcgc aggctgtggc
     6961 agctttcgag cccttcctcc tccaggaaga cctggagcat cgccggcacg aagtgcagca
     7021 gcgtgatccc ctgctccagt accgtcctgg ccagataccg gccctcccgc tggccacccg
     7081 gccgtgccag caccagacgg gcaccggcga gcagcggccc gaagcactcc cagaccgaga
     7141 agtcgaagct gaacggcgcc ttctgcagca cccggtccgc ggcggtcacc ggataggcgc
     7201 gctccgccca cagcagacgg ttcaccacgc cccggtggcg caccatcacc cccttggggc
     7261 gtcccgtcga gcccgaggtg tagatgatgt acagcaggct gtcaggcacc gcgccgccca
     7321 gcggccgctc ctccggagct ccgggcgcga aggccgagtc cgcctcctcg ccatccagca
     7381 gcaggacccg cgtatcgccg gccggcagcc gcagcaccag gtggctctgg gcgagcagga
     7441 gggaggctcc ggagtcctcg atcatgtagg ccaggcgctc ggccggatag tccggatcga
     7501 ggggcacgta ggccccaccc gccttgagca ccgccagcag gccgaccacc atcgccggcg
     7561 agcgctcgac gcacatcgcc accggcacct cggggccgac ccccagctcc cgcaggcgcc
     7621 aggccaggcg gttggcccgc cgctccagct cggcgtagct gagctcctcc ccttcgaacg
     7681 agaccgccac cgcgtccggc gagcgctcca cctgcgctcc caccagctcg tgcaggcaca
     7741 tccccccggc atggtcttcg ccgctgtcgt tccactcgac cagcagctgg tgccgctccg
     7801 ccggatcgag cagcggcacc tcaccgatcc ggcacgcggg atcgctggcg actgcttcga
     7861 ggaagcggcc gagatgggag agcagccgcg ccgccgtgga acggtcgaac aggtccgcgt
     7921 cgtactccag cgtgcccgcc aggccgccgg ctgactcggc gagcgtcagc atcagctcga
     7981 acttcgccac ctccagatcc acggggagcg gtgacagctt cagccccgga atcccggttc
     8041 caccctgcgg cacgttctgg aacgcgaaca tcacctggaa cagcggcgag cgggtcaggt
     8101 cccgctccgg cgccaccgcc tcgaccagcc gctcgaacgg cagcgcctgg tgcgcgaagg
     8161 cgtccagcgt cgtggtacgg gcccgcccca gcagctcgtg gaacggcggc gaccccgcca
     8221 actcggcccg cagcaccagg gtgttgacga agaagccgat cagcccctcg atctcccggc
     8281 ggttgcgtcc cgccaccggc gtcccgatca gcacgtcgtc ctgacccgcc cagcgcccga
     8341 gcagggcctg gaaggcggac gtcagggtca tgaacagcgt cgccccctcg cgccggctca
     8401 gctcggacag agcggcggtc agcgccgcgg ggatcaccgc cgggagcgtc ccgccggcgc
     8461 cgctgcggac agccggacgc ggccggtcga gcggcagctc cagggcctgc ggcgctccgg
     8521 ccagccgctc gcgccagtac gccagctccc gctccagcac ctccccctcg agccagcggc
     8581 gctgccagac ggcgaagtcg gcgtactgca ccggaagctc cgggagacgg ggcgcctcgc
     8641 cggccaggat cgcggcgtag cagcggatcc actcctcgaa gaagatcgtc cccgaccacc
     8701 cgtcggtggc gatgtggtgg gtgctggtga gaagcaccca gtcctc
//
LOCUS       DASPJI010000739         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_725417, whole genome shotgun sequence.
ACCESSION   DASPJI010000739 DASPJI010000000
VERSION     DASPJI010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725417"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="VEL74_20735"
     CDS             complement(<1..1545)
                     /locus_tag="VEL74_20735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG65021.1"
                     /translation="MRRLPLSRLSLRLLAFNLLLVFLPAAGFLYLDVYESQLLVAQER
                     AMVQQGRILAAALAEQGELDAAGAEALLARMEQRTEARLRVIGRGGAVLADTSRLGPR
                     AEDQPVPGPSTSRTDPYESSSPSPVRRETRESLLYRGGVVLYRLWRTVLPEPGPDTDS
                     RASRTSRPAGALDPEVAAALAGRYGAEARRNPGDPRSMTLHSVIPVRSGGEVVGAVRV
                     SQSTFRILGALVEVRLDIFKVFLASVAAAAVLSLLVSTTIARPLKRLRDEATALVDRR
                     GRLKGSFRGSRRRDEIGDLARALEQLTRQLEGHLRFTETFAADVSHEFKNPLAAIRGA
                     TELLAEVEDPAERARLLGMVERDVARMERLLSGVREITEIDARLEGEGAPALDLTGLL
                     GEVVESYRLRAGRGVRIDLQAPAGPLVVRASPERLAQVFENLLDNAMGFSPPGGTVTL
                     EVAPDDHFARVLVADQGPGIPEAHLERIFDRFFSYRPGEPHARNGHTGLGLAIARAIA
                     EGYGGTI"
     gene            complement(1542..>2021)
                     /locus_tag="VEL74_20740"
     CDS             complement(1542..>2021)
                     /locus_tag="VEL74_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011840309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYG65022.1"
                     /translation="RDEELDRVLGLELGADDYLCKPFSMRELVARVKVLLRRAAWREA
                     PAGQGEADERVLEAGRLRLDLRRYQAHWAGAPVALTVTEFTLLQALARHPGHVKSRAQ
                     LLQEAYPNDAYVSDRTIDSHVKRLRKKLAEADPRADSSSDPIETIYGLGYRWREEGG"
BASE COUNT          276 a          716 c          775 g          254 t
ORIGIN      
        1 gatcgtgccg ccgtatccct ccgcgatggc gcgggcgatg gcgaggccca ggccggtgtg
       61 gccgttgcgg gcgtggggct ctcccggccg atagctgaag aagcggtcga agatgcgctc
      121 caggtgggcc tccgggatgc ccggcccctg gtcggcgacg agcacgcggg cgaagtggtc
      181 gtccggcgcc acctccaggg tcaccgtgcc gcccggaggg gaaaagccca tcgcgttgtc
      241 gagcaggttc tcgaagacct gggccaggcg ctccggtgag gcgcgcacga cgagcggccc
      301 ggctggcgcc tggagatcga tccgcacccc gcggccggcg cgcagacggt agctctccac
      361 cacctcgccc agcaggccgg tgaggtccag ggccggcgcc ccctcgccct ccagccgggc
      421 gtcgatctcg gtgatctcgc gcacgccgga cagcaggcgc tccatgcgag ccacgtcccg
      481 ctcgaccatg ccgaggagcc gcgcccgctc ggcggggtcc tccacctcgg cgaggagctc
      541 ggtggcgccg cggatggcgg ccagcgggtt cttgaactcg tgcgagacgt cggcggcgaa
      601 ggtctcggtg aagcgcaggt gcccttcgag ctggcgggtg agctgctcca gggcgcgggc
      661 gaggtcgccg atctcgtcgc ggcgccgcga gccgcggaag ctccccttga gccggccgcg
      721 ccggtccacc agggcggtgg cctcgtcgcg caggcgcttg agcggcctgg caatggtggt
      781 ggagaccagc aggctgagga ccgccgccgc cgccaccgag gcgaggaaga ccttgaagat
      841 atccagccgg acctcgacca gggctcccag gatgcggaac gtggactgcg acacccgcac
      901 cgcccccacc acctcgccgc cgctgcgcac cgggatgacg ctgtggaggg tcatggaccg
      961 cgggtctccc ggattgcgcc gggcctccgc gccgtagcgt ccggccaggg ccgcggccac
     1021 ctcgggatcg agggcgccgg ccggccggga cgtccgggag gcccggctat cggtgtccgg
     1081 cccgggctcg ggcagaaccg tgcgccagag ccggtagagg acaacgccgc cccggtagag
     1141 aaggctctcc cgcgtctccc tccggacggg ggacggggag gaggattcgt aaggatcggt
     1201 gcgggacgtg gaggggccgg gcaccggctg atcctcggcc cgcggcccca gccggctggt
     1261 gtcggcgagc acggcgccgc cgcggccgat cacccgcagc cgcgcttcgg tccgctgctc
     1321 catgcgcgcc aggagggcct cggcgccggc ggcgtccagc tccccctgct cggcgagggc
     1381 ggccgccagg atgcgcccct gctgcaccat ggcccgctcc tgcgccacca ggagctgcga
     1441 ctcgtagaca tccaggtaga ggaagccggc ggccggcagg aagaccagca gcaggttgaa
     1501 ggccaggagc cggagcgaca gccgggagag ggggaggcgt ctcacccgcc ctcctcgcgc
     1561 cagcgatagc ccagcccgta gatcgtctcg atgggatcgg atgacgaatc ggcccgggga
     1621 tcggcctcgg cgagcttctt gcgcagacgc ttgacgtggc tgtcgatggt gcggtcgctg
     1681 acgtaggcgt cgttggggta ggcctcctgg aggagctggg cgcgggattt gacgtgtccc
     1741 gggtggcggg cgagggcctg gagcagggtg aactcggtga cggtgagggc gaccggcgcg
     1801 cccgcccagt gcgcctgata gcgccgcagg tcgagacgca ggcggccggc ctcgaggacc
     1861 cgctcgtcgg cctcaccttg gcccgcgggg gcctcgcgcc aggcggcccg acgcagcagc
     1921 accttgaccc tcgcgaccag ctcgcgcatc gagaacggct tgcacaggta gtcgtcggcc
     1981 cccagctcca ggccgagcac gcggtcgagc tcctcgtcgc g
//
LOCUS       DASPJI010000740         3854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_446812, whole genome shotgun sequence.
ACCESSION   DASPJI010000740 DASPJI010000000
VERSION     DASPJI010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446812"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VEL74_20745"
     CDS             <1..673
                     /locus_tag="VEL74_20745"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG65023.1"
                     /translation="SIAASGSGPAEMPAAEPPRFMTWADPAPFAELAEPAMPLPETEL
                     TMPEPEPVAPEPEPFALEPFPAAEPAPPVFPEAAPFDMETPEAPAWEPEPVPAPALFE
                     PEPVPVEDLEPPVYAAPEPGYAEPAATMTLGELYLRQGHLQEARRIFGEILEREPDHA
                     AAHDGLERVGQALTARERRPVEASELLAGFDPDAAESGANARKVFLLNSYLARVRRGR
                     QRDVS"
     gene            663..1019
                     /locus_tag="VEL74_20750"
     CDS             663..1019
                     /locus_tag="VEL74_20750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65024.1"
                     /translation="MFLERLNTISSRIDGALALSLVARDGMRVESVSSDPDLDLETLA
                     AELVAQARSISDNHRELEVGEVQQLAVTTDRLTLMVSSVAKDYYLLLVLGPDGSYGRA
                     RFELRRARLLLEEDLS"
     gene            1098..1601
                     /gene="accB"
                     /locus_tag="VEL74_20755"
     CDS             1098..1601
                     /gene="accB"
                     /locus_tag="VEL74_20755"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HYG65025.1"
                     /translation="MTFEQIKELIELVAQRQLQGLEIERSGFRLKVDGRTPVTAPAAA
                     PAPPADTTTYPAAPAQPAAAPAPAPQASAPAAAPASGPAAQPPGTHILTSPIVGTFYR
                     APSPDAAPFAEVGARVKKGQVLCIIEAMKLMNEIESDADGVIAEIYPQNAQAVEYGEP
                     LFAIRPS"
     gene            1612..2991
                     /gene="accC"
                     /locus_tag="VEL74_20760"
     CDS             1612..2991
                     /gene="accC"
                     /locus_tag="VEL74_20760"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HYG65026.1"
                     /translation="MFKKILIANRGEIALRIIQACKELGIQTVAVYSTADRDSLHVTY
                     ADEDVCIGPPPSRSSYLNISSIISAAEITGADAIHPGYGFLAENAHFAEVLEECKIAW
                     IGPRPEVIRLMGDKAKARQTAMAAGVPVLPGSPEPLADADEAMRLAAEVGYPVILKAA
                     AGGGGRGMRIVWEEKDLAGQFSTAREEAEKAFGDGSVYFEKYLVEPRHIEFQVFGDLH
                     GNVIHLGERECSIQRRHQKLIEEAPSPALTPELREKMGEAAVALCRSVRYENAGTIEF
                     LLDSDGRFYFMEMNTRIQVEHPVTEMVTGLDLVKMQIQVAAGDKLSVPSGIKPRGHAI
                     ECRVNAENPDNFAPSPGPLRTFHLPGGPGVRVDTHGYEDYVIPPHYDSLVAKLIVHDR
                     NREAAIARMSRALDFFVVEGIQTSIPLHRRILRDADFAAGKFSTRFMERFLQRAKEAK
                     ADEGGGEGK"
     gene            2988..3635
                     /locus_tag="VEL74_20765"
     CDS             2988..3635
                     /locus_tag="VEL74_20765"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HYG65027.1"
                     /translation="MTSSPEALRVPGRVYAIGDAGALGEVPLPEAVAAMAGEGIGWIQ
                     VRAKKMPGLELFRAVEACCRAVEGTGAALWIDDRADLAAIFPVAGLHLGQADLPPGAA
                     RRVVGGDLWIGFSTHGEEQLAAADADRAVDVVAVGPVFPTTSKERPDPVVGLELVRHA
                     RQRTAKPLVAIGGIDAGNLAEVLAAGADAAVVLGAVCRGDIRANCRRLLSAAAAA"
     gene            3654..>3854
                     /locus_tag="VEL74_20770"
     CDS             3654..>3854
                     /locus_tag="VEL74_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HYG65028.1"
                     /translation="MRIFLTGFMGSGKTTVGRLLAERLGLPFVDLDEEIELRVGLRVR
                     EIFELHGEPAFRQMEHEALRAVL"
BASE COUNT          555 a         1374 c         1403 g          522 t
ORIGIN      
        1 gtccatcgcc gcttcgggca gcgggcctgc cgagatgccg gccgccgagc cgccgcggtt
       61 catgacctgg gccgatccgg cgccgttcgc ggagctggcc gagccggcga tgccgctgcc
      121 ggagaccgag ctgaccatgc cggagcccga gccggtcgcc ccggagccgg agccgttcgc
      181 gctggagcct ttcccggccg cggagcccgc gccgccggtc ttccccgagg cggcgccgtt
      241 cgacatggag actccggaag ccccggcctg ggagcccgag cccgtcccgg ccccggcgct
      301 cttcgagccg gagccggtcc cggtagagga cctggagccg ccggtctatg ccgcgccgga
      361 gccgggctat gccgagccgg cggccaccat gaccctgggc gagctctacc tgcgccaggg
      421 gcacctgcag gaagcccggc gcatcttcgg cgagatcctg gagcgcgagc cggaccacgc
      481 cgccgcccac gacgggctgg agcgggtcgg ccaggccctg acggcgcgcg agcgccgtcc
      541 ggtcgaggcc tccgagctgc tcgccggctt cgaccccgat gccgccgaga gcggggcgaa
      601 cgcccgcaag gtcttcctcc tcaacagcta tctcgcacgc gtgcgtcggg ggaggcagcg
      661 cgatgtttct tgagcgtctg aacacgatca gcagccggat cgacggggcc ctggcgctct
      721 cgctggtcgc ccgcgacggc atgcgggtgg agtcggtgag ctccgatccc gacctcgacc
      781 tggagaccct cgccgccgag ctggtggcgc aggcgcggtc catctccgac aaccatcgcg
      841 agctcgaggt gggcgaggtg cagcagctcg ccgtcaccac cgaccggctg acgctgatgg
      901 tcagctcggt cgcgaaggac tattatttgc tcctcgtgct gggaccggac ggcagctacg
      961 gccgggcccg gttcgagctc cggagggcgc gcctgctcct ggaagaagac ctctcctgag
     1021 gtcggtccgg gccctggagt cccgggacgg gggcagcgcg gtttgcctgt gtcacttcgc
     1081 agagggactt cgccttgctg acatttgaac agatcaagga gctgatcgag ctggtcgccc
     1141 agcgccaact ccaggggctg gagatcgagc gctcgggctt ccgcctcaag gtcgacggcc
     1201 gcaccccggt gacggcgccg gccgccgcgc cggcgccgcc ggccgatacc accacttatc
     1261 ccgccgcgcc ggcgcagccg gccgcggctc ccgcgccggc cccgcaggcg tccgccccgg
     1321 ctgccgctcc ggcgtccggc cccgcggcac agccgccggg tacgcacatc ctcacctcgc
     1381 ccatcgtcgg caccttctac cgcgcgccct ccccggacgc cgccccgttc gccgaggtcg
     1441 gggcgcgggt caagaagggg caggtgctct gcatcatcga ggcgatgaag ctgatgaacg
     1501 agatcgagtc cgacgccgac ggcgtgatcg ccgagatcta tccgcagaac gcgcaggcgg
     1561 tcgagtacgg cgagccgctc ttcgcgatcc ggcccagcta gaggcccccg gatgttcaag
     1621 aagatcctga tcgccaaccg gggcgagatc gcgctccgca tcatccaggc gtgcaaggag
     1681 ctgggcatcc agacggtcgc ggtgtacagc accgccgacc gggacagcct gcacgttacc
     1741 tatgccgacg aggacgtctg catcggccct ccgccgtcgc ggtcgagcta cctcaacatc
     1801 tcgagcatca tctccgccgc cgagatcacc ggcgccgacg ccatccaccc gggctacggg
     1861 ttcctggccg agaacgccca cttcgccgaa gtgctggagg agtgcaagat cgcctggatc
     1921 ggcccgcgcc ccgaggtcat ccgcctgatg ggggacaagg ccaaggcgcg gcagacggcc
     1981 atggccgccg gcgtgccggt gctccccggc agccccgagc cgctggccga cgccgacgag
     2041 gccatgcgcc tggcggccga ggtcggctac cccgtcatcc tcaaggcggc ggcggggggc
     2101 ggcggccgcg gcatgcgcat cgtctgggaa gagaaggacc tggccggcca gttctccacc
     2161 gcccgcgagg aggcggagaa ggcgttcggc gacggctcgg tctacttcga gaagtacctg
     2221 gtcgagccgc ggcacatcga gttccaggtc ttcggcgacc tgcacggcaa cgtcatccac
     2281 ctgggcgagc gcgagtgctc catccagcgg cgccaccaga agctgatcga ggaggcgccg
     2341 tcgccggccc tcacccccga gctgcgggag aagatgggcg aggcggcggt ggcgctctgc
     2401 cggtcggtgc gctacgagaa cgccggcacc atcgagttcc tgctcgacag cgacgggcgc
     2461 ttctacttca tggagatgaa cacccgcatc caggtggagc acccggtgac cgagatggtg
     2521 accggcctcg acctggtgaa gatgcagatc caggtggcgg cgggcgacaa gctctccgta
     2581 ccgagcggca tcaagccgcg cggccacgcc atcgagtgcc gggtcaacgc cgagaacccc
     2641 gacaacttcg cccccagccc cgggccgctg cgcaccttcc atctccccgg cggccccggc
     2701 gtgcgggtgg acactcacgg ttacgaggac tacgtcatcc cgccgcacta cgactcgctg
     2761 gtggcgaagc tgatcgtcca cgaccggaac cgcgaggcgg ccatcgcccg catgagccgc
     2821 gccctggact tcttcgtcgt cgaggggatc cagacctcga tcccgctcca ccggcggatc
     2881 ctgcgcgacg cggacttcgc ggcgggcaag ttctccaccc ggttcatgga gcgcttcctc
     2941 cagcgggcca aggaagcgaa ggcggacgaa gggggcggcg aggggaaatg acgtccagcc
     3001 ccgaggctct ccgggtgccc ggccgggtct acgccatcgg cgacgccgga gccttggggg
     3061 aggtcccctt gcccgaagcg gtggccgcca tggcggggga ggggatcggc tggatccagg
     3121 tgcgggccaa gaagatgccg ggcctggagc tgttccgcgc cgtcgaggcc tgctgccgtg
     3181 cggtcgaagg gaccggcgcg gcgctgtgga tcgacgaccg ggcggacctg gcggcgatct
     3241 tccccgtcgc cgggctgcac ctggggcagg cggacctgcc gccgggagcg gcgcggcggg
     3301 tggtgggcgg cgacctctgg atcggcttct ccacccacgg cgaggagcag ctcgcggcgg
     3361 cggacgccga tcgggcggtc gacgtggtgg cggtggggcc ggtgtttccc accacgagca
     3421 aggagcggcc ggacccggtg gtggggctcg agctcgtgcg ccacgcccgg cagcggaccg
     3481 cgaagccgct ggtggcgatc ggcggcatcg acgcgggcaa cctcgcggag gtgctggcgg
     3541 ccggagcgga cgcggcggtc gtcctcggcg ctgtctgccg gggggacatc cgggccaact
     3601 gccggcggct cctgtcggcc gcggccgctg cctgagacgg gtgctgaaac gaagtgcgga
     3661 tctttctcac cggcttcatg gggagcggca agacgaccgt cggccggctg ctcgccgagc
     3721 gtctcggcct gccgttcgtc gatctggacg aggagatcga gctccgggtg gggctgcggg
     3781 tgcgggagat cttcgagctc cacggcgagc cggcgttccg gcagatggag cacgaggccc
     3841 tgcgcgccgt gctc
//
LOCUS       DASPJI010000741        10764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_560950, whole genome shotgun sequence.
ACCESSION   DASPJI010000741 DASPJI010000000
VERSION     DASPJI010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10764)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10764
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560950"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VEL74_20775"
     CDS             <1..531
                     /locus_tag="VEL74_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYG65029.1"
                     /translation="TVFANAGINGVWAPIDELEPEEWEKTISINLSGTFYTIKAAVPH
                     LKRQGGSVVVTSSVNGTRMFSNSGASAYATTKAGQVALTQMLSLELAKHRIRVNVICP
                     GAITTEIEESTEHRDQEKAKEPVEYPEGEIPLTDGKPGSSEQVARLVLFLASDDADHI
                     SGTPVWIDGAQSLLQG"
     gene            complement(538..1197)
                     /locus_tag="VEL74_20780"
     CDS             complement(538..1197)
                     /locus_tag="VEL74_20780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65030.1"
                     /translation="MSTDEDLAALHRRTSDPAHRAGCPAPGALLPLVSGSAAPAERER
                     LVDHVAGCTACAEEVRLMRSLRPWSEGLARRLEGGEPRRMGRRLLPLSLAAALLAAVV
                     GSAFVLRWDLLVPPDAGRLRSAPAERVSPAPDAVLQEVPAELAWEPQTGATGYRLRIF
                     DEEANRLWEGAILSETKGAIPPEARSRLRPGASYFWTVEVEGPARRRELGPYWFRLAD
                     P"
     gene            complement(1194..1763)
                     /locus_tag="VEL74_20785"
     CDS             complement(1194..1763)
                     /locus_tag="VEL74_20785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYG65031.1"
                     /translation="MPDPAPVRAVPEADHRLQVILAEYGGWLRRTLRKLCPTRRGIQV
                     DDLEQEVAIRIWRSLRDGRGIENPASFLYRVAATATVDAVRRVEARREEPLAAGGEEE
                     EGAAPPQTPGPDPEAAARSAELGRRLREALAALPDNRRRAVALHLQGFNVQEAAELLG
                     WTEAKTRNLIYRGLEDLRRDLGPTQGTLP"
     gene            complement(1838..2581)
                     /locus_tag="VEL74_20790"
     CDS             complement(1838..2581)
                     /locus_tag="VEL74_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65032.1"
                     /translation="MIRSNKMLALLALGLAAAVPGEAADPQVVHKVELQSGAKAVTIE
                     QRFVPVANGTDVQLIARGPVTGAKTVAVMYANGELAVKVDKAWWKSFFSANSLTGEAA
                     LLPVEDGGRTLAALTVSGGGSSHLIGVSMVDPDNPWGPCPGPDDVPTCRPEAVVIPFW
                     CKLDVDQCSGGPLPGPWAIRATTDPDGDPMLERGNATDLNGDPARGLAILRFSLDAAE
                     LSVNGTLDLGTTALSIDGSQGFDYRGPGF"
     gene            complement(2701..3594)
                     /locus_tag="VEL74_20795"
     CDS             complement(2701..3594)
                     /locus_tag="VEL74_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HYG65033.1"
                     /translation="MRLVTRGDLDGLTCAVLLSLNEEIDSIALIHPQDISDGRAEIRS
                     DDIIANLPYHPDCAMWFDHHLHTATPNLPPEGFKGVFAEAPSAARLVYDYYGGEEAMP
                     QLADLVYETDRLDSAQLTPDDVMAPQGFIKLGFTIDGRTGLGTFERYFLHLVDLLKSG
                     TPIEQVLEDPQVKRRCQIIESENEHFCQALRNHSRVEGNVVITDFRDLDHTPVGNRFL
                     VYALFPEVNVSARVHWGPNRSFPMLLLGHSIFRRTCKTNVGELASRYGGGGHRGAGSI
                     PLLDEPDQQIQMVVRELQANG"
     gene            3667..4989
                     /locus_tag="VEL74_20800"
     CDS             3667..4989
                     /locus_tag="VEL74_20800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYG65034.1"
                     /translation="MSTPATAFDLASLARESGSASQRVSGMASRLIGSEILRISGEIK
                     ALAATGRSLCDLTVGDFNPRYFPIPERLGAAIHTALDRGETNYPPSSGIAELRQAVAR
                     FYERALGLAYPVESVLIAGGARPVIYCIFQTVCDPGDRVVYPVPSWNNNHYSHLAGAV
                     GVPVVCAPEDRFMPTREALLEALPGARLLCLNSPLNPAGTVIGREALLGICEMVLEEN
                     EARGRRGERPLYLMYDQIYWLLTFGGAEHLTPPGLLPEVARYTLFVDGISKAFAATGL
                     RVGWGVGPVDVISRMSAVLGHIGAWAPRPEQVATVALLDDPAAIRDFEAAFQPEVEKR
                     LDRLHAGFQAMKERGLPADSVPPMGAIYLSARLHPFGRRTPDGAELRTNDDIRRYVLE
                     AAGVAIVPFQAFGSVAEDGWFRLSVGAVSMAEIEAALPRLEEALRRLS"
     gene            complement(5379..5537)
                     /locus_tag="VEL74_20805"
     CDS             complement(5379..5537)
                     /locus_tag="VEL74_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65035.1"
                     /translation="MAPHYFAAALSYFATAPKCSRTAPHHFAAAPKYYRAAPKRYETA
                     PYYFAAVP"
     gene            5858..7384
                     /locus_tag="VEL74_20810"
     CDS             5858..7384
                     /locus_tag="VEL74_20810"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HYG65036.1"
                     /translation="METLPGLFGIQAAEAPDAPALIFEDHVVTYGELAARAERLAVCL
                     QDFGVGPEVVVGILSGHCIERVVGIIGVLMAGGAWLPLSPSDPWERVTSLLTDAAAPV
                     LLAPAGSLSTPLPEGIRVLLFDGEPSMVPSSNPFLTGEGARSKADPDNLAVVLYSSVP
                     HGALEGAAVPHCGLSNRLLRICEAYDIRPRDVTLHQAASGSEALVWELFGPLVSGARV
                     VLACPGTGQDLRSLARLMAEQGVTLASLEALLDQPDVAACATALRQVFAGSEAVPAGL
                     PAFPAAAGFQAPPGRADGPGIRIDVLDAEQAPVAAGIPGELCLGGVGLARGYLGRPEL
                     TAERFLPDPFPPPAVAGGGRLYRTGNLVRSLANGKLQFLGRISEPVKSRVFLDRPAMA
                     EPFVTPRGDLERTIAAAWREVLRLPRVGVREQFFEVGGSSLLLPRLQGLLEKALGREL
                     AMMDLFRYPTIEELARHLADQKARETQPSRASQIRIRTQTRQEALLQLSNTRARHRRV
                     "
     gene            complement(7385..8116)
                     /locus_tag="VEL74_20815"
     CDS             complement(7385..8116)
                     /locus_tag="VEL74_20815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HYG65037.1"
                     /translation="MKLWKVGELARQTGLTVRTLHHYDEIGLLRPSHRSAAGHRLYGA
                     EDLARLLQILSLRQLGLSLEEIRECVTRPEFSLQRILDTHVARLRERIALQQKLCDRL
                     DAVAGHLRSAETVSVEELLQTMEAITMFEKYYTEEQLRTLEERHRQIGEDRIREVEAE
                     WPVLIAEVRVEMDRGTDPANETVQRLAARWQSLVAEFTGGDPGIAKSVGNLYREEPQA
                     RERVGLDPALFDYIGRAMAAMKKAE"
     gene            8297..8821
                     /locus_tag="VEL74_20820"
     CDS             8297..8821
                     /locus_tag="VEL74_20820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYG65038.1"
                     /translation="MDFDLTSSIAVLERTPHTLRAMLAGLPAAWTDANEGPETWSPYD
                     ILGHLIHGERVDWIERAQIILAQGPQRRFSPFDRFAQFRESQGRSLADLLDEFARLRA
                     ENLATLAGWSLTEAQLVLEGEHPDFGPVTLRQLLATWVAHDLGHVAQVARVMAKQYRE
                     AVGPWRAYLPIMDR"
     gene            complement(8917..9825)
                     /locus_tag="VEL74_20825"
     CDS             complement(8917..9825)
                     /locus_tag="VEL74_20825"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HYG65039.1"
                     /translation="MKTTVSRIGPLLAGILLALSSAGSAHAGGCGEASCNSAQGAYIS
                     HFSSPNCTGTESYYLPYDSFGYDCRSYDGGGECGTIQHTVTNYSYRDSSGSCHNAWPN
                     GNPLSNFVTVYRPRFCGEASCNAAQSAYISHFTGSNCTGTESYYLPYSSFGYQCRPHD
                     GDGECGTIQRTVTNRSYRDSSGNCYNAWPNGNPLSNFVTVYRTAWSPPPPPPPLPMPP
                     VACGYPLGMTSGTAPFGTSFSAGCSFDPDGSITGFLWSFGDGGTSSSPYPYHFYFWPG
                     FYSVYLSVVDNDGKSDTTYVGTIYAY"
     gene            complement(10071..>10764)
                     /locus_tag="VEL74_20830"
     CDS             complement(10071..>10764)
                     /locus_tag="VEL74_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015136941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYG65040.1"
                     /translation="YVRVQSQGDYDVLIVGGEDHKTGQADDTKERHVRLEAWARVRFP
                     RMGPVDFTWAGQVMETIDGLAFIGRNPMDKDNVYIVTGDSGMGMTHGTIAGILLTDLI
                     LGRDNRWATLYDPARKTLRAAGEYAKEALNMAAQYSDWVTGGDVGSTDEIAPDSGAVV
                     RRGLAKVAVYRDANGVLHERLAVCTHLGCIVSWNTSEKTWDCPCHGSRFDRFGKVLNG
                     PANRDLAEVEKA"
BASE COUNT         1624 a         3579 c         3817 g         1744 t
ORIGIN      
        1 acggtctttg ccaacgccgg gatcaacggc gtctgggcgc cgatcgacga gctcgagccg
       61 gaggagtggg agaagacgat ctccatcaac ctctccggaa cgttctacac gatcaaggcg
      121 gcggtgcccc atctcaagcg ccagggcggc tcggtggtgg tcacctcgtc ggtcaacggc
      181 acgcggatgt tcagcaacag cggcgcctcg gcctacgcca ccaccaaggc ggggcaggtc
      241 gctctcaccc agatgttgtc cctggagctc gcgaagcacc gcatccgcgt caacgtcatc
      301 tgccccgggg cgatcacgac ggagatcgag gagagcaccg agcaccgcga ccaggagaag
      361 gcgaaggagc cggtggagta tcccgagggg gagatcccgc tgaccgacgg caagccgggc
      421 agctccgaac aggtggcgcg gctggtcctc ttcctggcct ccgacgacgc tgatcacatc
      481 tccggcaccc cggtctggat cgacggcgcg cagtcgctgc tccaggggtg aggcccttca
      541 cgggtccgcc agccggaacc agtaggggcc gagctcgcgc cgccgggccg gcccttccac
      601 ttccacggtc cagaagtagg aggcgccggg gcgcagccgg gagcgcgcct cgggggggat
      661 ggcccccttc gtctccgaga ggatcgcccc ttcccacagc cggttcgcct cctcgtcgaa
      721 gatccgcagc cggtagccgg tggcgcccgt ctgcggctcc caggccagct ctgcgggcac
      781 ttcctggagg accgcgtcgg gagcggggct cacccgctcc gccggagcgc tccggagccg
      841 cccggcgtcc gggggcacga gcaggtccca ccgcaggacg aaagcggacc ccaccaccgc
      901 ggccagcagc gccgccgcca aggagagggg gaggaggcgt ctgcccatcc ggcgcggctc
      961 gccaccctcc agccgccggg ccaggccctc cgaccagggg cgcagcgagc gcatcagccg
     1021 cacctcctcg gcgcaggcgg tgcagccggc gacgtggtcc accaggcgct cccgctccgc
     1081 cggcgcggcc gatcccgaca ccagagggag gagagcgccg ggcgccgggc agccggcgcg
     1141 gtgggccggg tccgaggtcc ggcggtgcag cgccgcgagg tcttcgtccg tgctcatggg
     1201 agagtcccct gagtggggcc gaggtcccgg cggagatcct ccaggccccg gtagatgaga
     1261 ttgcgcgtct tggcctcggt ccagccgagg agctccgccg cctcctgaac gttgaagccc
     1321 tgcaggtgga gggccacggc ccgccgccgg ttgtccggca gggcggccag ggcctcgcgc
     1381 agccggcggc cgagctcggc ggagcgcgcc gcggcttccg gatcggggcc cggggtctgc
     1441 ggcggcgcgg ctccctcttc ctcctcaccg cccgcggcca gcggctcctc gcggcgcgcc
     1501 tccacccggc ggaccgcgtc gaccgttgcg gtcgccgcga cgcgatagag gaaggaggcg
     1561 gggttctcga tcccccggcc gtcccgcagg ctgcgccaga tgcggatcgc cacctcctgc
     1621 tccaggtcgt ccacctggat cccccggcgc gtcgggcaga gcttgcgcag ggtgcggcgc
     1681 agccagccgc cgtactcggc gaggatcacc tgcagccggt ggtcggcttc ggggaccgcg
     1741 cggacgggag cagggtcggg caaggctcgg gggcgagggt aggagagcgc cctcgccccc
     1801 gtcaaggccg gtgccggccc ggcgggcttc cggctcgtca gaagccgggc cctcggtagt
     1861 cgaacccttg gctgccgtcg atggaaaggg cggtggtgcc caggtccagc gtgccgttga
     1921 cggacagctc cgcggcgtcg aggctgaagc ggaggatcgc gagcccacgg gcaggatctc
     1981 cgttgaggtc tgtcgcgttg ccccgctcga gcatcggatc gccatcgggg tcggtggtgg
     2041 ccctgatggc ccaggggccc ggcagcgggc cgccggagca ctggtcgaca tcgagcttgc
     2101 accagaaggg gatcaccacc gcttccggcc ggcaggtcgg cacgtcgtcc gggccgggac
     2161 aggggcccca cgggttgtcc gggtcgacca tgctgacgcc gatgaggtgg gacgagccgc
     2221 cgccgctcac ggtgagggcg gcgagtgtgc ggccgccatc ctcgacgggg agcagcgcgg
     2281 cctcgccggt caggctgttg gcgctgaaga agctcttcca ccaagcctta tcgaccttca
     2341 ctgccaactc gccgttggcg tacatgaccg ccaccgtctt ggcgccggtc accgggccgc
     2401 gggcgatgag ctggacgtcc gtgccgttcg ccacggggac gaaccgctgc tcgatggtca
     2461 ccgccttggc gccgctctgg agctccacct tgtggaccac ctgggggtcc gcggcctcgc
     2521 cgggcaccgc cgccgccagg cccagggcca gcagggccag catcttgttg cttcggatca
     2581 cgatctacct ccttcgtctt ggttcctctg ggaccgggcc actcgcccgg cttcgagggg
     2641 acagacgcag gaggggggat ttttttctca cggcgcggag agggctctcc gcgccgtctc
     2701 tcagccgttg gcctggagct cgcgcacgac catctggatc tgctggtcgg gctcgtcgag
     2761 gagcgggatg ctgccggcgc cgcggtggcc gccgccgcca tagcgcgagg cgagctcgcc
     2821 gacgttggtc ttgcaggtcc gccggaagat gctgtggccg aggagcagca tggggaagct
     2881 gcggttcggg ccccagtgca cccgcgccga gacattgacc tccgggaaca gcgcgtagac
     2941 caggaagcgg ttgcccaccg gcgtgtggtc caggtcccgg aagtcggtga tgaccacgtt
     3001 gccctccacc cggctgtggt tgcgcagcgc ctggcagaag tgctcgttct cgctctcgat
     3061 gatctggcag cgccgcttca cctgcgggtc ctccagcacc tgctcgatcg gcgtgccgga
     3121 cttgagcagg tcgaccagat ggaggaagta gcgctcgaag gtgccgaggc cggtgcggcc
     3181 gtcgatggtg aagccgagct tgatgaagcc ctgcggggcc atgacgtcgt cgggcgtgag
     3241 ctgtgccgag tccagccggt cggtctcgta gaccaggtcg gcgagctgcg gcatggcctc
     3301 ctcgccgccg tagtagtcat agaccagccg cgccgccgag ggggcctcgg cgaagacccc
     3361 cttgaagccc tcggggggga ggttgggcgt ggccgtatgc agatggtggt cgaaccacat
     3421 ggcgcagtcc gggtggtacg ggaggttggc gatgatgtcg tcggaccgga tctcggcccg
     3481 cccatcgctg atgtcctggg ggtggatgag cgcgatgctg tcgatctcct cgttgaggga
     3541 gagcagcacg gcgcacgtga gaccgtcgag gtcaccgcgt gtcactaggc gcatgattgc
     3601 ccgtgagtat atgacggaat cggcggagct ggaaccccgg cgcgaatcct ggcatagtcc
     3661 aggtccatga gcactccagc gaccgctttc gacctcgcgt ccctcgcgcg cgaatccggc
     3721 tccgcctcgc aacgggtctc ggggatggcg tcccgtctga tcggctcgga gatcctccgc
     3781 atctcgggcg agatcaaggc cctggcggcc accggccgct ccctctgcga tctcacggtg
     3841 ggggacttca acccgcgcta cttcccgatc ccggagcgcc tcggcgcggc catccatacc
     3901 gccctggacc gcggggagac gaactacccg ccgtcgagcg gcatcgccga gctgcggcag
     3961 gcggtggcgc ggttctacga gcgggcgctg gggctggcct atcccgtgga gtcggtgctc
     4021 atcgcgggcg gcgcccggcc ggtgatctac tgcatcttcc agacggtgtg cgatcccggc
     4081 gaccgggtgg tctatccggt gcccagctgg aacaacaacc actattcgca cctggcgggg
     4141 gcggtggggg tgccggtggt ctgcgcgccg gaggaccggt tcatgccgac ccgcgaggcg
     4201 ttgctcgaag cgctccccgg cgcgcgcctc ctctgcctca actcgccgct caacccggcg
     4261 ggcacggtga tcggccgcga agcgctcctc ggcatctgcg agatggtgct ggaggagaac
     4321 gaggcgcggg ggcggcgcgg cgagcgcccg ctctacctca tgtacgacca gatctactgg
     4381 ctgctgacct tcggcggcgc cgagcacctg acgccgcccg gcctgctgcc ggaggtggcg
     4441 cgctacaccc tcttcgtcga cggcatcagc aaggccttcg ccgccaccgg cctgcgggtc
     4501 ggctggggcg tcggcccggt ggacgtcatc tcccgcatgt cggccgtgct gggccacatc
     4561 ggcgcctggg cgccgcgccc cgagcaggtg gcgacggtgg cgctgctcga cgacccggcg
     4621 gccatccgcg atttcgaggc cgccttccag cccgaggtgg agaagcggct cgaccgtctc
     4681 cacgccggct tccaggcgat gaaggagcgc ggcctgccgg cggacagcgt gccgccgatg
     4741 ggcgccatct acctgagcgc ccgcctccat cccttcggcc ggaggacccc cgacggcgcc
     4801 gagctgcgca ccaacgacga catccgccgc tacgtcctgg aagccgcggg agtcgccatc
     4861 gtccccttcc aggccttcgg ctccgtcgcc gaggacggct ggttccggct gagcgtcggc
     4921 gcggtctcca tggcggagat cgaagcggcc ctgccgcgcc tggaagaggc gctgcgccgc
     4981 ctctcgtagc ccctcctccc agcaggcaaa gccatatccg aacgcggcac cccccggcgg
     5041 ggtgccgcgc tccggacggg tacgtcgatt gtcaaagaac ccgaggagcc ggagccgtga
     5101 aacggctggg ccgctcctcg cgacgtgggg agcataccac ggaagctcaa gtgctgactg
     5161 gattgacgcc ggtgaggtcg aatgtgtaca ttggcccccc tcgagggcga ctccagagca
     5221 gtagcgaagt acttcggagt catcctggag cacttcggag cggctccgca gtacttccgg
     5281 gccgcagcga agtaccacgg gagggcagcg aagtagtacg gcgtcatccc gcagcgctgc
     5341 ggattggatt cgtagtacgt cgagggcgcg gcgaagtact acgggacggc ggcgaagtag
     5401 tacggagccg tctcgtaacg cttcggagcg gccctgtagt acttcggggc ggcggcgaag
     5461 tggtgcgggg ccgttcttga gcacttcgga gccgtggcga agtagctcag ggcggcggcg
     5521 aagtagtgcg gagccattcc tgaacacttc ggagcggctt cgtagtgctc cggagccgcg
     5581 gcgaagcagt acgggacggc ggcgaagtac tacggcgcgg cgccggatcg agctcattga
     5641 caactgaatc gtgaggggcg cgcgccgcct gatccaaggc cgcccccctg aggatccctc
     5701 catccgacgg gggggatcgt gtgggcggtg ctgggaggat ctatggttaa attgctacaa
     5761 tgctaggcag gaggaacggc gacctcaagg atcactgcat atgcctcacc ggctacttgg
     5821 caatcggtcc cgcgcggaga cgcgttgcgc agcagagatg gagacgctcc ctgggctgtt
     5881 cgggattcag gcggcggagg cccctgatgc ccctgccctg attttcgaag accacgtggt
     5941 gacctacggc gagctagctg cgcgggcgga gcggcttgcc gtctgcctgc aagactttgg
     6001 cgtcgggccc gaagtagtgg tgggcatcct ctccgggcac tgcatcgaga gggtcgtcgg
     6061 gatcatcggc gtgctcatgg cgggcggtgc ctggcttccg ttgagccctt cggatccatg
     6121 ggaaagggtg acgtcgctgc tgacagacgc cgcggcgccg gtgctgctgg ccccggcggg
     6181 ctctctgtcc actccactcc cggaaggcat ccgcgttctt cttttcgatg gcgagccctc
     6241 gatggttccc tcttccaacc cttttctgac aggagagggg gcacggtcga aggccgaccc
     6301 cgacaacttg gcggttgtgc tctactcctc ggtaccccac ggcgctctcg agggtgccgc
     6361 ggtgccgcac tgcgggctga gcaaccgcct gctgcggata tgcgaggcct acgacatcag
     6421 gccaagggac gtcactctgc accaggcagc gagtgggtct gaggccttgg tctgggagct
     6481 gttcggtccg ctcgtctccg gagcccgggt agtcttggca tgtcctggaa cgggtcagga
     6541 tctccggtcg ctggctcgcc tgatggcaga gcagggagtg acgctcgcgt ctctggaagc
     6601 gctcctcgat cagccggatg ttgccgcgtg cgccaccgct ctgcggcaag tcttcgccgg
     6661 tagcgaggcc gtgccggccg gactgcccgc gtttcccgcc gctgccggct ttcaggcgcc
     6721 accaggcaga gcggacggtc cggggatccg gatcgacgtc ctggacgccg agcaggcacc
     6781 ggtggccgca ggcatcccgg gcgagctctg tctgggaggg gttggcctgg cccgtggcta
     6841 cctcgggcgg cccgagctta ccgccgagcg cttcctgccg gacccgttcc cgccgccggc
     6901 ggtagcgggc ggcgggcgtc tctatcggac aggcaatctg gtgcgttccc tggccaacgg
     6961 caagttgcag ttcctgggac gaatcagcga accggtgaag agccgcgttt tcctggaccg
     7021 ccccgccatg gccgagcctt tcgtgacgcc gcgcggggac ctcgaacgga cgatcgcggc
     7081 tgcctggcgg gaggtgttgc gtctcccacg cgtgggcgtc cgcgagcagt tcttcgaggt
     7141 cggggggagc tcactcctcc ttcccagact gcaagggctg ctggagaagg cattgggcag
     7201 ggagttggcg atgatggatc tgttccgcta ccccacgatc gaggagctgg ctcgtcacct
     7261 ggcagaccag aaggcgcgcg aaacccagcc aagccgggcc agccagatcc gcatccgtac
     7321 gcagacacgc caggaggcgc tgctccaact cagcaatacc cgggcccggc accgccgggt
     7381 gtgatcactc cgccttcttc atagccgcca tcgcccggcc gatgtaatcg aagagggccg
     7441 ggtcgagccc cactcgctcg cgggcctggg gctcttcccg gtagaggttg cccaccgact
     7501 tcgcgatgcc gggatcgccg ccggtgaact ccgcgaccag ggactgccag cgggcggcga
     7561 ggcgttgcac ggtctcgttc gccgggtcgg tgccgcggtc catctccacc cgcacctcgg
     7621 cgatgaggac gggccactcg gcctcgacct cgcggatgcg gtcctcgccg atctggcggt
     7681 ggcgctcctc gagggtgcgg agttgctctt cggtgtagta cttctcgaac atggtgatcg
     7741 cctccatggt ttgcaggagc tcctcgacgg agaccgtctc cgccgagcgc aggtgcccgg
     7801 cgaccgcgtc cagccggtcg cagagcttct gttgcagcgc gatccgctcc cggagccggg
     7861 cgacatgggt gtcgaggatc cgctgcaggg agaattccgg gcgcgtgacg cactcccgga
     7921 tctcctccaa cgagaggccg agctggcgca gcgacaggat ctggagcagg cgggcgaggt
     7981 cctcggcacc gtacagccgg tgccccgcgg cgctacgatg cgacggccgg agcaggccga
     8041 tctcgtcgta gtggtgcagg gtgcggacgg tcagccccgt ctgcctggcg agctcgccca
     8101 ccttccagag cttcatcacg gtttcctttc tcggctcctc tctccggagg agcggccatg
     8161 gacacgacgc tacagcctca cgttacgtga ggtgcaagag gggaagtcgc cctcggctga
     8221 gcccaggggg cgctctcctt gtgcctcctc attctccggc tataagctcg ctgcccacgt
     8281 catagaaagg aagcccatgg atttcgacct gacctccagc atcgccgtcc tcgaacgcac
     8341 gccgcacacc ttgcgtgcga tgctagccgg gctcccggct gcgtggaccg acgcgaatga
     8401 aggtccggag acgtggagcc cctatgacat cctgggtcac ctaatccatg gcgagcgagt
     8461 cgactggata gagcgggcgc agatcatcct cgcccagggc ccgcagcgcc gcttcagccc
     8521 cttcgaccgc ttcgctcagt tccgtgaaag ccaggggagg tctctagccg atctgctcga
     8581 cgaattcgcg cgcctgcgag ccgagaacct tgccacgctt gccgggtgga gtctgacgga
     8641 ggcccagctc gtcctggagg gggagcatcc cgacttcggg ccggtgacgc tacgccaact
     8701 gctcgccacg tgggtcgcgc acgacctggg gcacgtggcg caggtggccc gcgtcatggc
     8761 gaagcagtac cgtgaggcgg tcggaccgtg gcgcgcgtac ctcccgatca tggaccggtg
     8821 aggagagccg cgggagctgg gagcgggtgc gccccgaggt gtgggatcac ggcgcccccg
     8881 ctctctcgcg agaggcgggg ggcgccgtgc gcggaatcaa taggcgtaga tcgttccgac
     8941 gtaagtggtg tcggatttgc cgtcgttgtc aacgaccgac agatacaccg aatagaatcc
     9001 gggccagaag tagaagtggt agggataggg ggaagagctg gttccgccat cgccgaagct
     9061 ccacaggaag ccggtgatcg aaccgtctgg atcgaagctg caaccggcgc tgaaggaggt
     9121 gccgaacgga gccgtccccg aggtcatgcc caacgggtag ccgcaagcca ccgggggcat
     9181 gggcagaggg gggggagggg gcggaggaga ccaggcagtc cggtagaccg tgacgaagtt
     9241 gctgagggga ttgccattgg gccatgcgtt gtagcaatta ccgctgctgt ctcgatagga
     9301 acgattggtg acggtacgct gaatcgttcc gcactcgcca tcaccatcgt ggggccggca
     9361 ttgatagccg aagctgctgt acggcaggta gtaggattcg gtgccggtgc agttggagcc
     9421 ggtaaaatgg ctgatgtacg ctgattgcgc cgcgttgcaa ctcgcttcgc cacaaaacct
     9481 tggccggtag accgtgacga agttgctgag gggattgccg ttgggccatg cgttatggca
     9541 actaccgctg ctgtccctat aggagtagtt ggtgacggtg tgctggatgg ttccgcattc
     9601 gccgccgccg tcgtaggacc ggcagtcgta gccgaagctg tcatacggca ggtagtagga
     9661 ctcggtgccg gtgcaattag ggctggaaaa gtggctgatg tatgccccct gtgccgaatt
     9721 gcagctcgct tcgccgcacc ctcccgcgtg tgcggagccg gcggagctga gggcaagaag
     9781 gatccccgcc agaagcgggc cgattctact gacagtcgtt ttcatggcga cctccaaagt
     9841 tggcgctgac gacctgatag ctggatcgca ccggaagcac gtggtggggg caggcatctc
     9901 cctcaccacg gcgaagactg accgtctctg cggcgcgaca gcggtggctc tactggaatg
     9961 agtgcctggt cagggggaaa gcggtactac tactcccggg gggcgaggcg caggtatcgc
    10021 ctcctccagg cggagggcgc tacggccttg cgtcgcctag ccggctctgc ctaggccttt
    10081 tccacctcgg ccaggtcccg gttggcggga ccgttcagaa ccttgccgaa tctgtcgaag
    10141 cgcgagccgt ggcaggggca gtcccaggtc ttctcgctcg tgttccaact gacgatacag
    10201 ccgagatggg tgcagaccgc cagacgctcg tgcaggaccc cgttcgcgtc acggtagacc
    10261 gccaccttcg ccagcccgcg gcgcaccacg gcgccgctgt cgggggcgat ctcgtcggtg
    10321 gagcccacat cgccgccagt cacccagtct gaatactggg cggccatgtt gagcgcctcc
    10381 ttggcgtact cgcccgcggc gcggagcgtc ttgcgcgcgg ggtcgtagag ggtcgcccag
    10441 cggttgtccc ggcccaggat caggtccgtc aggaggatgc cggcgatcgt gccgtgcgtc
    10501 atccccatgc cggaatcgcc cgtgacgatg tagacgttgt ccttgtccat gggattgcgg
    10561 ccgatgaacg cgaggccgtc gatcgtctcc atgacctggc cggcccaggt gaagtcgacc
    10621 ggccccatcc ttggaaagcg cacgcgggcc caggcttcca gccggacgtg ccgttctttc
    10681 gtatcgtccg cctggcccgt cttgtggtcc tccccgccga cgatcagcac gtcgtagtcc
    10741 ccctgcgact ggacccgcac gtag
//
LOCUS       DASPJI010000742         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_47848, whole genome shotgun sequence.
ACCESSION   DASPJI010000742 DASPJI010000000
VERSION     DASPJI010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47848"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VEL74_20835"
     CDS             <1..265
                     /locus_tag="VEL74_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65041.1"
                     /translation="GDRAHFWPFDGFEVPRNRSVIAEVRPARLRHRYQQDELNRWELE
                     AYAICAWLQERDRLDLLAPYFKPPLSDHEQERARLEGWILGVA"
     gene            270..1979
                     /locus_tag="VEL74_20840"
     CDS             270..1979
                     /locus_tag="VEL74_20840"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HYG65042.1"
                     /translation="MAVSEKPAASTSTPAAPDPHATHAVFNQAPPYEEVNLFTSDRVL
                     GEAVVREGAGWAAERLRGFGAVCGRRETLELGVRANERTPVLRTHDRRGVRVDEVELD
                     PSWHALMRLGVENGLHALPWREPRSGAHVARAALFMLLTEVEAGVGCPLSMTYSAVPA
                     LRQQPDLAAEWEPRFTSLSYEPRLIPAADKPGALCGMAMTEKQGGSDVRANTTAARPL
                     PEGGDGEYLLTGHKWFCSAPMCDAFLVLAQAISEGRGGLSCFLVPRFTPDGRRNRFHL
                     QRLKDKLGNRSNASCEVEFRDTWARRVGEEGRGVPTILEMVNHTRLDCVIGSAAGMRA
                     AVARALHHAAHRQAFGKLLADQPLMRNVLADLALESEAATVSMMRLARAYDEATAGDE
                     TAASFRRLATAVVKYWVCKRTAVHAAEALECLGGNGYVEEWGMARLYREAPLNSIWEG
                     SGNVQCLDVLRALQKSPEALDALLAEVEAARGADPRLDAFAARLRDGLASHDDVEVRA
                     RRLVEELALALQASLLIRCSPAAVAGAFCASRLAGDSGRAFGTLPAGTAFGAILERHG
                     PAT"
BASE COUNT          285 a          831 c          789 g          319 t
ORIGIN      
        1 cggggatcgg gcccacttct ggcccttcga cggcttcgag gtgccgcgga accggtcggt
       61 catcgccgag gtgcgtccgg cgcgtcttcg ccaccgttac cagcaggacg agctgaaccg
      121 ctgggagctg gaggcctacg ccatctgcgc ctggctccag gagcgcgacc gcctcgacct
      181 cctcgccccc tacttcaagc cccccctctc ggaccacgag caggagcggg cccggctgga
      241 gggatggatc ctgggtgtag cctagaggca tggccgtctc cgagaagccc gccgcctcca
      301 cctccacccc cgctgcgccg gacccccacg cgacccatgc cgtcttcaac caggcgcctc
      361 cttatgagga ggtgaacctg ttcacgtcgg accgggtgct gggggaggcg gtggtccggg
      421 agggggcagg gtgggcggcc gagcggctgc ggggcttcgg cgcggtgtgc gggcggcgcg
      481 agacgctgga gctgggggtg cgggccaacg agcggacgcc ggtgctgcgc acgcacgaca
      541 ggcggggcgt ccgcgtcgac gaggtggagc tggacccgtc ctggcacgct ctgatgcggc
      601 tgggcgtcga aaacggtctc cacgcgctgc cctggcgcga gccccgctcg ggcgcccacg
      661 tggcccgggc ggcgctgttc atgctcctga ccgaggtgga ggcgggggtg ggatgccccc
      721 tctccatgac ctactcggcg gtgccggccc tgcgccagca gccggacctc gccgccgaat
      781 gggagccccg cttcacctcc ctctcctatg agccccggct gatccccgcg gcggacaagc
      841 ccggcgctct ctgcggcatg gcgatgaccg agaagcaggg gggctcggac gtgcgggcca
      901 acaccacggc cgcgcggccg ctgccggagg ggggcgacgg ggagtacctg ctcaccggcc
      961 acaaatggtt ctgctcggcc ccgatgtgcg acgccttcct ggtgctcgcc caggcgatat
     1021 ctgaagggag gggagggctg tcctgcttcc tggtgccgcg cttcaccccg gacggccggc
     1081 gcaaccgctt ccacctccag cggctcaaag acaagctggg caaccgctcc aacgcctcct
     1141 gcgaggtgga attccgcgac acctgggccc gccgcgtggg ggaggagggg cggggcgtgc
     1201 ccacgatcct cgagatggtg aaccacacgc ggctcgactg cgtcatcggc tcggccgccg
     1261 ggatgcgtgc cgccgtggcc cgggccctcc atcacgccgc ccaccgccag gccttcggga
     1321 agctcctcgc cgatcagccc ttgatgcgca acgtcctcgc cgacctggcc ctggagtccg
     1381 aggccgccac ggtctccatg atgcgcctcg cccgcgccta cgacgaggcg acggcagggg
     1441 acgagaccgc cgcctccttc cggcgcctgg ccacggccgt cgtcaagtac tgggtctgca
     1501 agcggaccgc cgtccacgcc gccgaggccc tggagtgcct ggggggcaac ggctatgtag
     1561 aggaatgggg gatggcccgc ctctaccgcg aagcccccct caattccatc tgggagggct
     1621 cgggcaacgt ccagtgtctg gacgtcctgc gcgccctcca gaaatccccc gaagcgctcg
     1681 acgccctgct cgccgaggtc gaggcggccc ggggcgccga cccccggctc gacgccttcg
     1741 ccgcccggct gcgcgacggc ctcgccagcc acgacgacgt cgaggtccgc gcccgccgcc
     1801 tggtggagga gctggcgctg gccctccagg cctccctcct gatccgctgc tccccggccg
     1861 ccgtggccgg cgccttctgc gcctcccgcc tggccggcga ctcggggcgc gctttcggca
     1921 ccctgccggc ggggaccgcc ttcggggcga tcctggaacg gcacggcccg gcgacataag
     1981 agagatcccc gggcttgccg ctgggcggca gggcgtgtag agttgagtca ggagtcccct
     2041 aacagatgat tcagccgctg tctgtcgttt ctgtgccctc gtccgcgcac gaggaggatt
     2101 tcgacctctc gcccccggcc tggacggagc tcctgtccgg cggaggcttc ttccgcacca
     2161 gcgcccgggt gcgctgggct cgcgggctcg ggccggcggc cttccgcgag gccaccaccc
     2221 gcgc
//
LOCUS       DASPJI010000743         4775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_218073, whole genome shotgun sequence.
ACCESSION   DASPJI010000743 DASPJI010000000
VERSION     DASPJI010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218073"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VEL74_20845"
     CDS             complement(<1..254)
                     /locus_tag="VEL74_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYG65043.1"
                     /translation="MRFRDRVALVTGGGSGIGAAAARAFAAEGARVAVLGRTREKLDE
                     VVEAIQKDGGEAMTVTADVSKYAEMEEAVRQVVDRWGRLDT"
     gene            complement(376..954)
                     /locus_tag="VEL74_20850"
     CDS             complement(376..954)
                     /locus_tag="VEL74_20850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65044.1"
                     /translation="MRNSKLLILLMILVLPLAIACGREGAETENANDVTTGATEQEQA
                     GVEGTSDVSPMTAEMAIDDIVVGSGLAPDGTIASESREDDFTPGEDVYVAMEVGDVPA
                     GSAVKVVWYQNAGGQTQDDNAAAGDATTDKGGTQVGEETKTVTAGQSYLNFQAPSTGS
                     WTLGDYRVEVWYGDEKVAEEQFQIVEQSDSAT"
     gene            complement(1032..2348)
                     /locus_tag="VEL74_20855"
     CDS             complement(1032..2348)
                     /locus_tag="VEL74_20855"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HYG65045.1"
                     /translation="MRAPSIDDLVLLPEPLALQRHYDAKNYRTFRWLWALTALVSVIG
                     IIAAAKGGMPLRLVLFVLGLVGSVALFGMRQSPLFERHFRQILLSYLAFQLVLIKLAP
                     SMDDGFALFAMLMLFFRFRVTEFLILFGASWITAILPPGWVGLAEAGAPRSEDFAELS
                     VMHGIFLGLAVLLTHFERRRFLGGWRREHGRSRERLRMREEIEYARKIQLSMLPQAPP
                     EAGWIELAGASLPATEVGGDYYDYFALSPSRLALVVGDVSGHGLACGLLLSGVRSCLY
                     LMEEDLATPALALQRLNRMVRRTTDRRTYVTLLCALVDREAGTLTVASAGHPPVVCFD
                     PRSRTLQEMGQGAPPLGTFLKAQYQEESRPIARGDLLVFYTDGLTEARNSQGVEYGVE
                     RLQKVISHTWDAPVREIRDAVLSDLSRFKGDGEQADDITVVIARLK"
     gene            2391..3221
                     /locus_tag="VEL74_20860"
     CDS             2391..3221
                     /locus_tag="VEL74_20860"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HYG65046.1"
                     /translation="MEEDVRTLAEALRRAGNGLVVAVTGAGVSAASGLPTFRGKDLGA
                     IWSRDVTEVGTFRYFQRDPVAWWQWFADRFAGIAEARPNAGHAALVALERWQSARRGD
                     FLLITQNIDSLHEDAGSRRLIKVHGSANRVRCSRHGCLNGAPSGSLPVADSGLDAFLA
                     DPRRESLPRCPACGSLLRPHALLFDEYYEEHTDYGYSEVRRSLERMALVLFVGTSFSV
                     GVTELVLREALGWRLPAFSIDPAAAPPAPGITGLRAAAEEILPAVCRELGIEDAAPTP
                     "
     gene            complement(3211..4134)
                     /locus_tag="VEL74_20865"
     CDS             complement(3211..4134)
                     /locus_tag="VEL74_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HYG65047.1"
                     /translation="MRFLLACPGCSRQFDVTGHAVGSRLSCSCGGVLTVPQPQGHDAA
                     VVRCSSCGAPREIGAATCRFCSSEFTLHEKDLDTLCPGCMARVSRRARFCHGCGTPVL
                     GAQASRPGTDLRCPSCPPRPEPRALASRRLGRANVAVFDCGVCGGLWVDREIFEVMVE
                     RARAGSLPELGTSGPVSLPPPEKPANPDGQAAYRPCVVCGALMHRRNYGKKSGIILDV
                     CSRHGVWFDLHEMDRLLRWIREGGEERTRALEGEAAREAARRLERAKGVEPLRLTRED
                     RRQELADGSLFGAVLYFLTGLTTGFGDLFRE"
     gene            complement(4155..>4775)
                     /locus_tag="VEL74_20870"
     CDS             complement(4155..>4775)
                     /locus_tag="VEL74_20870"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYG65048.1"
                     /translation="GDVRNRDLGEEPDMQTYWPQLQTPVVSAALVVRAGSGREPEDLA
                     GVLRQVVTAADKEIPVDQVRSLEEVVSQALAQSRFKTVLLGLFAGLALVLAAVGVYGV
                     ISYSVAQRTHEIGIRMALGAHRGQVLRMVVRQGMGLVLAGVIAGLVLAWFAVRLLTDQ
                     VYGVSPSDPATFALVPLVLLAVALLANYLPARRATRVDPLEALRYE"
BASE COUNT          707 a         1709 c         1671 g          688 t
ORIGIN      
        1 gtgtcgagcc tcccccagcg gtccaccacc tggcggaccg cctcctccat ctcggcatac
       61 ttggagacgt ccgcggtgac ggtcatggcc tcgccgccat ccttctggat ggcttcgacg
      121 acctcgtcca gcttctcgcg cgtccgtccc agcaccgcca cccgcgctcc ttcagcggcg
      181 aaagccctgg ccgccgccgc gccgatgccg gagccgccac cggtcaccag ggccactctg
      241 tctcgaaacc tcatactgcc tcaccttctg ctgcataaac gcaaaaagac ggcggctctg
      301 gctcaagccg ccgttgggag aatagattgc cgctcctgcg ttggcttctg tttcctgtgt
      361 tcccagcccg agctcctagg tggccgagtc ggactgctcg acgatctgga actgctcctc
      421 ggcgaccttc tcgtcgccgt accagacctc gacccggtag tcgccgaggg tccacgagcc
      481 ggtgctcggg gcctggaagt tcaggtagga ctgaccggcg gtcacggtct tggtctcctc
      541 gccgacctgg gtgccgccct tgtcggtggt ggcgtcgccc gcggcggcgt tgtcgtcctg
      601 ggtctgcccg ccggcgttct ggtaccagac gaccttgacg gccgacccgg cgggcacgtc
      661 gcccacttcc atcgccacgt agacgtcctc acccggggtg aagtcgtcct cgcggctctc
      721 ggaggcgatg gtgccgtccg gggcgaggcc gctgcccacg acgatgtcgt cgatcgccat
      781 ctcggcggtc atcggcgaga cgtcgctggt cccctcgacg ccggcctgct cctgctcggt
      841 ggcgccggtg gtcacgtcgt tcgcgttctc cgtctcggcg ccctcgcggc cgcaggcgat
      901 cgccagcggc aggacgagga tcatcaacag aatcaacagc ttgctgttac gcatgcccta
      961 tctccttttt tggcttggct agtactgcac caactgccag gcccaaacag caagggccgt
     1021 gccagggagg gctacttgag ccgggcgatg accacggtga tgtcgtcggc ctgctcgccg
     1081 tcgcccttga agcgggagag gtcggagagg acggcgtcac ggatctcacg gacgggggcg
     1141 tcccaggtat gggagatcac cttctggagg cgctcgacgc cgtactccac cccctggctg
     1201 ttgcgggcct cggtgagtcc gtcggtgtag aagaccagga ggtcgccgcg ggcgatggga
     1261 cgcgactcct cctgatactg cgccttgagg aaggtgccga gggggggcgc gccctgcccc
     1321 atctcctgga gggtacggct ccgcggatcg aagcagacca ccggcggatg gccggcgctc
     1381 gccacggtga gggtgccggc ctcgcggtcg accagggcgc agaggagggt gacgtaggtg
     1441 cgccggtcgg tggtccgccg gaccatgcgg ttgagccgct gcagcgccag cgccggtgtc
     1501 gccaggtcct cctccatcag atagaggcag ctccgcacgc cggagagcag gaggccgcag
     1561 gcgagaccgt gccccgaaac gtcgccgacg accagcgcca gccgcgacgg cgagagggcg
     1621 aagtagtcgt agtagtcgcc ccccacctcg gtggccggca gcgacgcgcc ggccagctcg
     1681 atccagccgg cctcgggcgg cgcctgcggc agcatgctga gctggatctt gcgggcgtat
     1741 tcgatctcct cgcgcatccg cagccgctcg cgggagcggc cgtgctcccg ccgccagccc
     1801 cccaggaagc gccgccgctc gaagtgcgtc agcaggacgg ccagccccag gaagatgccg
     1861 tgcatgacac tgagctcggc gaagtcttcg ctgcgcggcg cccccgcctc cgccagcccg
     1921 acccagcccg gagggaggat cgcggtgatc cacgaggcgc cgaacaggat caggaactcc
     1981 gtgaccctga aacggaagaa cagcatcagc atcgcgaaga gggcgaaccc gtcgtccatg
     2041 gagggggcga gcttgatgag gacgagctgg aaggctagat aggacagcag gatctgacgg
     2101 aagtgccgct cgaacagggg tgactggcgc atgccgaaca acgcgaccga tcccaccagg
     2161 ccgaggacga agaggacgag ccgcaggggc atcccgcctt tggccgcggc gatgatgccg
     2221 atgaccgaga cgagggcggt cagcgcccag agccaccgga aggtgcggta gttcttggcg
     2281 tcgtagtgcc gctgcagcgc caggggctcc ggcagcagca cgagatcgtc gatcgagggg
     2341 gctctcatcc agggcggttc cgttccgggg taatgctatc ctgccgcggc atggaagagg
     2401 acgtgcggac actggccgaa gctctgcggc gagcggggaa cggcttggtg gtcgccgtca
     2461 ccggcgccgg cgtcagcgcg gccagcggcc tcccgacctt ccgcggcaag gacctggggg
     2521 cgatctggag ccgagacgtc accgaggtcg gcaccttccg ctacttccag cgcgatccgg
     2581 tggcctggtg gcagtggttc gccgaccgct tcgccggcat cgcggaggcg cggccgaacg
     2641 ccgggcacgc cgccctggtc gccctcgagc gctggcagtc ggcccgccgc ggggacttcc
     2701 tgctgatcac ccagaacatc gacagcctgc acgaggacgc cggctcgcgc cggctgatca
     2761 aggtccacgg cagcgccaac cgggtgcgct gctcgcgcca cggctgcctg aacggcgccc
     2821 cctccggctc gctgccggtg gccgactccg gcctcgacgc cttcctggcc gacccgcggc
     2881 gcgagtcgct cccccgctgc cccgcctgcg gctcgctgct ccgccctcat gccctgctgt
     2941 tcgacgagta ctatgaagaa catacggact atggctactc cgaggtgcgg cggtcgctcg
     3001 agcgcatggc cctcgtcctg ttcgtcggga catcgttctc cgtcggcgtg acggagctgg
     3061 tgctgcgcga agccctgggc tggcggctgc ccgcgttctc gatcgacccg gccgccgcgc
     3121 cgccggcgcc cgggatcacg ggtctccgcg ccgccgccga ggagatcctc cccgccgtct
     3181 gccgggagct gggaatcgag gacgccgcac ctactccctg aacagatcac cgaaaccggt
     3241 ggtcagcccg gtcaggaagt acaggacggc gccgaagagc gagccgtcgg ccagctcctg
     3301 gcggcggtcc tcgcgggtga gccgtagcgg ctccaccccc ttcgcccgct ccagccggcg
     3361 ggccgcttcg cgcgcggcct cgccttccag cgcccgggtg cgctcctcgc ccccctcgcg
     3421 gatccagcgc agcagccggt ccatctcgtg gaggtcgaac cagacgccgt ggcgggagca
     3481 gacgtcgagg atgatgccgc tcttcttgcc atagttgcgg cggtgcatga gcgccccgca
     3541 gacgacgcat ggacggtagg ccgcctggcc gtccgggttg gccggcttct ccggcggcgg
     3601 caggctgacc ggcccgctgg tgccgagctc gggcaggctg ccggcgcgcg cccgctccac
     3661 catcacctcg aagatctcgc ggtccaccca caggccgccg cagacgccgc agtcgaagac
     3721 cgccacgttg gccctgccca gccgccggct ggcgagtgcc ctcggttccg ggcggggtgg
     3781 gcaggagggg cagcggagat ctgtcccggg gcgggaggcc tgagccccca ggaccggggt
     3841 gccgcagccg tggcagaagc gggcgcggcg gctcacccgc gccatgcagc ccgggcagag
     3901 ggtgtccaga tccttctcgt ggagggtgaa ctcggagctg cagaagcggc aggtagccgc
     3961 accgatctcg cgcggcgcgc cgcaagacga gcagcgtacc accgccgcgt cgtgcccctg
     4021 cggctggggg accgtgagca cgccgccgca cgagcaggac agccgcgagc ccacggcgtg
     4081 gccggtcacg tcgaactggc ggctgcagcc cgggcaggcg aggaggaagc gcactcgaga
     4141 gtctccagag tcttctattc gtatcgcaac gcttccagcg ggtctacccg ggtagcgcgg
     4201 cgggccggca ggtagttggc gagcagggcc acggcgagca gcaccagcgg caccagcgcg
     4261 aaggtcgccg ggtccgaggg gctgacgccg tagacctgat cggtgagcag ccgcaccgcg
     4321 aaccaggcca gcaccagccc cgcgatcacc cccgccagca ccaggcccat cccctgccgc
     4381 accaccatgc gcagcacctg cccgcggtgg gcgcccaggg ccatccggat gccgatctcg
     4441 tgggtgcgct gcgccaccga gtagctgatg acgccgtaga cccccaccgc ggccagcacc
     4501 agggcgaggc cggcgaagag gccgagcagc acggtcttga agcggctctg ggcaagggcc
     4561 tgggagacca cctcctccag gctccgcacc tggtcgacgg ggatctcctt gtcggcggcg
     4621 gtgaccacct gacgcaggac gccggcgagg tcctcgggct cccggccgct gcccgcgcgc
     4681 accaccaggg cggcggagac gacgggcgtc tggagctgcg gccagtaggt ctgcatgtcc
     4741 ggctcttcgc cgagatcgcg gttgcgcacg tcccc
//
LOCUS       DASPJI010000744         3796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_446905, whole genome shotgun sequence.
ACCESSION   DASPJI010000744 DASPJI010000000
VERSION     DASPJI010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446905"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /locus_tag="VEL74_20875"
     CDS             <1..1323
                     /locus_tag="VEL74_20875"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HYG65049.1"
                     /translation="LAGKGRELVLYTLTAAKNEAAPLGKLHGQYLQRLPSAVARLMAD
                     GGTTPPPTPTLPTAPGNLQASALSATEVQLSWSDNSSNEDGFRVERSVAGGAFLEVQT
                     AGTNATGARVTGLAAATAYTFRVRAANSAGFSAYSNSAAATTLTAPVTPAAPAAPTTL
                     QAQALSGTEVQLTWTDSSSNEDGFRVERSTAGGAFLEIQTVAANATGSRVTGLTAGTA
                     YTFRVRAANAAGLSAYSNSASATTTAPVATTVPAAPSSLAAKATSATQIQLTWRDNSG
                     NEASFRIERIINGRWTEIASVAANSTSASVSGLIASTSYSFRVRAANSAGYSPYSNTA
                     KSSTLRSSVTPPAAPSNFSVTYLGSGTVRFTWTDNSTNETNFRIERMIGGVYKEVLVT
                     GANLNTVNLGGLTDGASYNFRMRASNGAGYSGYSNGDNVNMWKGRRRR"
     gene            1489..2421
                     /locus_tag="VEL74_20880"
     CDS             1489..2421
                     /locus_tag="VEL74_20880"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HYG65050.1"
                     /translation="MTDSINLLYIMGHGYSGSTLLTFLLGAHPEIATVGEMGIAPKAK
                     GKGKPEEFLCSCKTPIRECDFWHQVTRGMEKQGYKFDVWETAHEFRANGGGVTDAVLR
                     AVQRGPLFETARRVALGTLPGARRELGRILRYNEALVRVVTGIKSCCTFLDSSKRPER
                     AVYLRRVPSFDVKVIHLVRDGRAVSYSSMKNLGLGPEAAAESWIADNRSSEHARRHFP
                     SDRWMSLRYEDLCANPPEVLGRIYQFAGVQPKNGMQDFRAVEQHIIGNRMRLSNTSEI
                     RLDEKWKTALTPEQMKAIESRVGAMNRRYGYDGL"
     gene            2408..>3796
                     /locus_tag="VEL74_20885"
     CDS             2408..>3796
                     /locus_tag="VEL74_20885"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYG65051.1"
                     /translation="MTVSETVSGVARTTLQERPVHKERSHDAERPAERTTRRPLRPGE
                     PARVAVVGAGYVANFHLEILAEMPDVQVVAVCDPALDRAREAAAKFHIPNAVASVDEL
                     PALGVEVAHVLVPPDLHVTVTRRLLELGIGALVEKPLALSSADGRELQRLAGERGLPL
                     GVNHNNVHHPAFSRLLKHVRMGRIGRLEHVQICFNMPLAQLEAGDFSHWMFRAPRNIV
                     FEQAPHPLSQLHALIGRVLSARTTLLGTRELLPGQVFHDRWLVAARGERGTAEIYLAF
                     GQPFQRWTIQVLGTDGSAEADLGNNFFSYEEKTPWLEFWNTFLAGTRRARSLAGDATA
                     NLGRYAGYTLGMTRRQDYYFVGMRRSVRAFYAALRAGRDIPGDAGQGAEVLEWCEAVA
                     EVAATPAASSQPALLPEPGPARPGEVVVLGGNGFIGRRVVSKLLERGLPVSVVVRRAH
                     SLPPEISEPARAG"
BASE COUNT          576 a         1442 c         1234 g          544 t
ORIGIN      
        1 ctcgccggca aggggcgcga gctggtgctc tacaccctga ctgcggcaaa gaacgaggcc
       61 gcccccctgg gcaagctcca cggccagtac ctgcagcgcc tcccctcggc ggtggcgcgc
      121 ctgatggcgg acggcggcac cacgccgcca ccgaccccga ccctcccgac ggcccccggc
      181 aatctccagg cttcggcgct ctccgccacc gaggtccagc tctcctggag cgacaactcc
      241 tccaacgagg acggcttccg ggtcgagcgc tcggtcgccg gcggcgcctt cctggaggtc
      301 cagaccgccg gcaccaacgc caccggtgcc cgcgtgaccg gcctcgcggc cgccacggcc
      361 tacaccttcc gtgtgcgcgc cgccaactcc gccggcttct cggcctacag caacagcgcc
      421 gcggccacga cgctgacggc gccggtcacc cccgcggccc cggcggctcc cacgaccctc
      481 caggcccagg cactctccgg cacggaggtc cagctcacct ggaccgacag ctcctccaat
      541 gaggatggct tccgggtcga gcgctcgacc gccggcggcg ccttcctgga gatccagacc
      601 gtcgccgcca acgccaccgg cagccgcgtg accggcctca cggcgggcac cgcctacacc
      661 ttccgcgtcc gcgccgccaa cgccgccggt ctctcggcct acagcaacag tgcctcggcc
      721 acgaccacag cgccggtagc caccacggtc ccggccgcgc ccagcagcct ggcggccaag
      781 gcgacctcgg ccacccagat ccagctcacc tggcgggaca actccggcaa cgaggcgagc
      841 ttccgcatcg aacgcatcat caacggccgg tggaccgaga tcgccagcgt cgccgccaac
      901 tccaccagcg ccagcgtctc cggcctcatc gcgtcgacct cctactcgtt ccgcgtgcgg
      961 gcggccaact ccgccggcta ttcgccctac agcaacaccg ccaaaagctc gacgctgcgg
     1021 agctcggtca caccgcccgc cgcgccgagc aacttctcgg tcacctacct ggggagcggc
     1081 acggtccggt tcacctggac cgacaactcc accaacgaga ccaacttccg gatcgaacgg
     1141 atgatcggcg gggtctacaa ggaggtcctg gtgaccggtg ccaacctcaa caccgtgaac
     1201 ctcggcgggc tcaccgatgg cgcctcctac aacttccgca tgcgggcgag caacggcgcc
     1261 ggctactcgg gctatagcaa cggcgacaac gtcaatatgt ggaaggggcg ccggcgccgc
     1321 tgatccctca gtcccttcgc gtcccttccc ctcggggtga gcggccgggt ctcccggccg
     1381 ctttttttat ggcatgggtc ctgcacaagt tggccgtcgc agaagttgta tccgtggaat
     1441 cgctcccccg ggggtgcacg ccgtagtgaa gcaaggagag ccttgtagat gaccgactcg
     1501 atcaatctcc tctacatcat gggccacggc tattcgggct cgacgctcct gacgttcctg
     1561 ctcggggcgc atcccgaaat cgccacggtg ggtgagatgg gcattgcccc caaggccaag
     1621 ggcaagggca aaccggagga gttcctttgc tcttgcaaga ccccgatccg ggagtgtgat
     1681 ttctggcacc aggtcacccg ggggatggaa aagcagggct acaagttcga cgtctgggag
     1741 accgcccacg agttccgggc caacggcggc ggcgtgaccg acgccgtcct gcgtgcggtg
     1801 cagcgcgggc cgctgttcga gaccgcgcgc cgggtcgccc tcggcaccct tccgggggcc
     1861 cgccgcgagc tcggccgcat cctccgctac aacgaggccc tggtgcgggt ggtcaccggc
     1921 atcaagagct gctgcacgtt cctcgacagc tcgaagcgcc cggaacgcgc cgtctacctc
     1981 cggcgggtcc ccagcttcga cgtcaaggtg atccacctcg tgcgcgacgg ccgggccgtc
     2041 tcctactcct cgatgaagaa cctgggcctc ggccccgagg cagcggcgga gtcgtggatc
     2101 gccgacaacc ggtcctcgga gcatgcccgg cgccacttcc cctccgatcg ctggatgtcc
     2161 ctgcgctacg aggacctctg cgccaatccg cccgaggtcc tcggccgcat ctaccagttc
     2221 gccggcgtgc agccgaagaa cgggatgcag gatttccgcg ccgtggagca gcacatcatc
     2281 ggcaaccgca tgcggctctc caacacctcg gagatccgtc tcgacgagaa atggaagacc
     2341 gccctcaccc ccgagcagat gaaggccatc gagagccggg tgggggcgat gaaccggagg
     2401 tatggctatg acggtctctg agacggtctc cggggtggcg cggaccaccc tccaggagcg
     2461 tcctgttcat aaggaaaggt cccacgacgc ggagcgcccc gccgagagga cgacgcgccg
     2521 gccgctccgc ccgggtgagc cggcgcgggt ggccgtggtg ggcgccggct acgtcgccaa
     2581 cttccatctg gagatcctgg ccgagatgcc ggacgtccag gtggtcgcgg tctgcgaccc
     2641 cgccctcgac cgggcgcggg aagcggcggc gaagttccac atcccgaacg cggtcgcctc
     2701 ggtggacgag ttgcctgccc tgggcgtcga ggtcgcgcac gtcctcgtgc cgccggacct
     2761 gcacgttacg gtgacccgcc ggctgctgga gctggggatc ggcgcgctgg tcgagaagcc
     2821 tctggccctc tccagcgccg acggccgcga gctgcagcgg ctcgccggcg agcgcgggct
     2881 ccccctgggg gtcaaccaca acaacgtcca tcacccggcg ttctcccgcc tcctcaagca
     2941 cgtgcgcatg gggcgcatcg gccggctgga gcatgtccag atctgcttca acatgcctct
     3001 cgcccagctc gaggcgggcg acttctcgca ctggatgttc cgcgcgccgc gcaacatcgt
     3061 cttcgagcag gcgccccacc cgctgagcca gctccacgcc ctgatcggcc gggtgctgtc
     3121 ggcgcgaacg accctcctcg gcacgcgcga gctgctgccg gggcaggtct tccacgaccg
     3181 ctggctggtg gccgcccggg gtgagcgcgg cacggcggag atctacctcg ccttcggcca
     3241 gccgttccag cgctggacga tccaggtcct tggcaccgac ggctcggccg aggccgacct
     3301 gggcaacaac ttcttctcct acgaggagaa gaccccctgg ctcgagttct ggaacacctt
     3361 cctggcgggc acccgccgag cccgctcgct ggccggcgac gccaccgcga acctcggccg
     3421 ctacgcgggc tacacgctgg gcatgacccg gcgtcaggac tactacttcg tcggcatgcg
     3481 ccgctcggtg cgggccttct acgcggcgct ccgcgccggc cgggacatcc ccggcgacgc
     3541 cggccaggga gccgaggtgc tggagtggtg cgaagccgtg gctgaagtgg ccgcgacgcc
     3601 ggccgcgtcc tcccagcccg cgctgctgcc ggagcccggg cccgcgcggc cgggcgaggt
     3661 ggtggtgctc ggcggcaacg gcttcatcgg ccggcgggtg gtgagcaagc tcctggagcg
     3721 cggcctcccc gtctcggtgg tggtccgccg cgcccacagc ctgccccccg agatctccga
     3781 gccggcgcgc gccggc
//
LOCUS       DASPJI010000745         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_561119, whole genome shotgun sequence.
ACCESSION   DASPJI010000745 DASPJI010000000
VERSION     DASPJI010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561119"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VEL74_20890"
     CDS             <1..564
                     /locus_tag="VEL74_20890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HYG65052.1"
                     /translation="ESVLGPDYKIVEIGFDVPGPGAVDQPWHRDFPAPDETLVGRRLN
                     SLAFNLTCVDVFEDMGPFEIAPGTQWDDPTEFEHGMFPPKSLYGRYEKRAQKKMPQMG
                     DISARSALTIHRGTPNRSNTSRPALVLGADAPGGINAERHDLQFTRGYYDRLPDELRR
                     HLTCRVVDELEPIVQAHTIEGLVMGAA"
     gene            complement(569..1786)
                     /locus_tag="VEL74_20895"
     CDS             complement(569..1786)
                     /locus_tag="VEL74_20895"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HYG65053.1"
                     /translation="MDWETRYFVSRQLRLRVQTPGLLVATTTRSPSEIKPEAIPVLLG
                     FSAAATPREVLERLRQEWEIDEESFVLVVDSMLAQGILVPAGGIADPLLSPALRGNGF
                     GSAVPHLRMLRDAVRVMSYRSAIERQAPGRTVVEIGCGSGILSIFAAKAGARKVIAIE
                     ETRIAEVAAEMFAASGCADRIELRVANSRDVELDEPADLIIHEILGVDPFEESILPVL
                     ADARRRLLRPGGRLLPYRLEVCGLGVEAVATVTDADRLRSETEELRHLYGLDFQPLLR
                     ALEESARNPASRRTVIAGKPHFEPKILSEECRFLDIDLRDDDIDLPGKSFRMPLKIVH
                     EGVLGGIAVFFRAHLDEQVQLTTSPHAPPTHWGWTIQNLNRRVPVSPGDQVEIALELK
                     DETGTQLVSLDLA"
     gene            1872..>2789
                     /locus_tag="VEL74_20900"
     CDS             1872..>2789
                     /locus_tag="VEL74_20900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65054.1"
                     /translation="MRRPVRRPVRRSRALVALPLAVLLAVPAAAQDDQPPLLPESVIR
                     VLAGELSGTEAKRTVQDLTLFHRMRGSRGFQAAAGRIRDRAREYELQQVEIVDLPADG
                     KVFYGTQRSRPAWDAEFAELWEQEKEEGGGWRDARLVTSWETRPVSLAQDSASGEAAA
                     ELVDAGAGTRESDYQGKDVKGKLVLVSSQPSSAAPLAVERFGAAGLVSYAQNQVSAWW
                     KEDENLVRWGHMGTFPAPRTFGFMVSLQQARAWQERLARGETVRLRASVRAGQHPGSY
                     GIPTAVLPGADPAVAGEEIVLSCHLDHQRP"
BASE COUNT          422 a         1022 c          954 g          391 t
ORIGIN      
        1 gagtccgtgc tcggcccgga ctacaagatc gtcgagatcg gcttcgacgt gcccgggccg
       61 ggcgccgtgg accagccctg gcaccgtgac ttccccgcgc ccgacgagac gctggtcggc
      121 cggcggctca actcgctggc cttcaacctc acctgcgtgg acgtgttcga ggacatgggg
      181 ccgttcgaga tcgcccccgg cacgcagtgg gacgacccca cggagttcga gcacggcatg
      241 ttcccgccga agtcgctcta cggccgttac gagaagcgcg cccagaagaa gatgccgcag
      301 atgggggaca tctcggcacg gtcggccctc accatccacc gcggcacgcc caaccggtcg
      361 aacacttcac gcccggcgct cgtcctcggc gccgacgcgc cggggggcat caacgccgaa
      421 cggcacgact tgcagtttac ccgggggtac tacgaccggc tgcccgacga gctccgccgg
      481 cacctgacgt gccgggtcgt cgacgagctc gagccgatcg tccaggctca caccatcgag
      541 ggcctggtga tgggcgcggc ctgacccgct acgccaagtc gaggctgacg agctgcgtcc
      601 cggtctcgtc cttgagctcc agcgcgatct ccacctggtc gccgggggag acggggacgc
      661 gccggttcag gttctggatg gtccaccccc agtgggtcgg cggcgcgtgc ggggaggtgg
      721 tgagctgcac ctgctcgtcg agatgggcgc ggaagaagac cgcgatcccc cccagcacgc
      781 cctcatggac gatcttcagg ggcatccgga agctcttgcc ggggaggtcg atgtcatcgt
      841 cccgcaggtc gatgtccaga aaccggcact cctcggacag gatcttcggc tcgaaatggg
      901 gcttgcccgc gatcacggtc cgccgggacg cgggattgcg cgccgactcc tccagggccc
      961 gcaacagagg ctggaagtcc agcccataga gatgacggag ctcctcggtc tcggagcgca
     1021 gccggtcggc atcggtcacc gtagccaccg cctcgacccc caggccgcag acctccaacc
     1081 ggtaggggag cagccgcccg ccgggccgga gcagacggcg ccgcgcgtcc gcgaggaccg
     1141 gcaggatgct ctcctcgaac ggatcgacgc ccaggatctc gtggatgatg aggtcggccg
     1201 gctcgtcgag ctccacgtcg cggctgttcg ccactcgcag ctcgatccgg tcggcacagc
     1261 cgctggccgc gaacatctcg gccgccacct cggcgatccg cgtctcctcg atggcgatca
     1321 ccttgcgggc gcccgccttg gcggcgaaga tcgagaggat gcccgagccg cagccgatct
     1381 cgaccaccgt cctgcccggc gcctgccgct cgatggccga ccggtagctc atcacgcgca
     1441 cggcgtcccg caacatcctg agatgcggca ccgcggagcc gaacccgttg ccacgcaggg
     1501 ccgggctcag gagaggatcg gcgatgccgc cggccggcac caggatcccc tgcgccagca
     1561 tcgaatcgac cacgagcacg aagctctcct cgtcgatctc ccactcctgc cggagccgct
     1621 ccagcacctc ccggggagtc gccgcggccg agaagccgag caggaccggg atggcttcgg
     1681 gcttgatctc gctcggcgag cgcgtcgtcg tcgccaccag caggcctggg gtctggaccc
     1741 tgagccgaag ctggcgcgaa acaaagtacc gggtctccca atccatccct gatccccttt
     1801 ccagagctcg cgagagtgta acccggtcca gggagtgata gcgtttcgcg cattctcagg
     1861 aggagcgtcc catgcgccga cccgtgcgcc ggcccgtccg ccggtcccga gccctcgtgg
     1921 ctctccctct cgccgtcctc ttggccgttc ctgccgcggc ccaggacgac cagccgccgc
     1981 tgctgcccga gtcggtgatc cgggtgctgg ccggcgagct gtcgggcacg gaggccaagc
     2041 ggaccgtcca ggacctcacc ctgttccacc ggatgcgcgg ctcccgcggc tttcaggccg
     2101 ccgcggggag gatccgcgac cgcgcccggg agtacgagct gcagcaggtg gagatcgtcg
     2161 acctcccggc cgacggcaag gtcttctacg gcacccagcg ctcccgtcca gcctgggacg
     2221 ccgagttcgc cgagctgtgg gagcaggaga aggaggaagg gggcggctgg cgcgacgccc
     2281 gcctcgtgac ctcctgggag acccggccgg tgagcttggc gcaggacagc gcctccggcg
     2341 aggccgcggc cgagctcgtg gacgccggcg cgggcacccg ggaatccgac taccagggca
     2401 aagacgtgaa ggggaagctc gtccttgtct cctcccagcc gagctcggcg gcaccgctcg
     2461 ccgtggagcg cttcggcgcg gccggcctgg tctcctatgc ccagaaccag gtgagcgcct
     2521 ggtggaagga ggacgagaac ctggtgcgct gggggcacat ggggaccttc ccggcgcccc
     2581 gcaccttcgg cttcatggtc tccctccagc aggcccgcgc ctggcaggag cgactcgccc
     2641 gcggcgagac ggtgcgcctg cgggcgagcg tccgcgccgg ccagcacccg ggctcgtacg
     2701 gcatacccac cgcggtcctc cccggcgcgg acccggcggt ggcgggggag gagatcgtcc
     2761 tctcctgcca tctcgaccac cagcggccc
//
LOCUS       DASPJI010000746         5145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_851857, whole genome shotgun sequence.
ACCESSION   DASPJI010000746 DASPJI010000000
VERSION     DASPJI010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851857"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>5145
                     /locus_tag="VEL74_20905"
     CDS             <1..>5145
                     /locus_tag="VEL74_20905"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65055.1"
                     /translation="TQAMSRVRQAFGVEVPLRALFEGPTVAELARVVEAGLADLATAG
                     PEAPPLVPMPAAMRAGELPLSFAQQRLWFLDRLVPDNPFYNMFGAVRLAGPVDVPALR
                     EACREIARRHEVLRTTFPSVAGRPVQAIAAEPPPGFPLIELGGLPEAPRREELERLAN
                     AEGRRPFDLTRGPVFRAALLRLGEAEHSLFFNLHHIVSDGWSLGVLHRDLAVLYGAFH
                     RRQLSPLPELPVQYADFAVWQRGWLQGEALRARLDYWRGQLEGMPEVLELPTDRPRPA
                     IESFRGAGAPFPLAAPLVRRLSLLARRRGATLSMVVLAGFKALLARLSGRGDVPVGVA
                     IAGRNRRETEELIGFFVNTLVLRTQVSGSAGSVGAGGFGDLVARVRETSLGAYAHQDL
                     PFEKLVEELAPDRDLGRNPLVQVTFGFHNFPRREVETGGLVLIPPSEEAVGTGTSKFD
                     LSLHLFEADGALQGTLEYNLDLFDEPTARRFTGHLATLLAAAVEDPEVEPALLPLLSE
                     AESHQLLTAWNDTASPYERDLTVHEVFDLQVEARPDAVAVIAGDEHLTYLGLARRARR
                     LAADLRALGVGPEEVVGLRAERSAGLPAAMLGILYAGAAYLPLDPEAPEERLALMREE
                     AGVRFVVSGGAVMPPAAPGQEGGSPSSLAYVIYTSGSTGRPKGVAVPHRGIVRLVRQA
                     GYARLDADETLLQVAPVSFDASTFEIWGALLNGGRLVMMPPEKPTLDGIGDALARHGV
                     TTLHLTAGLFHAMVEERLGALAGLRQLLTGGDVLSAPHVRRAAASLPHCQVIACYGPT
                     EGTTFTSVHLIDPNGAGHLDTSVPLGRPIGNTRVLVLDRSLLPVPVGVAGDLAVGGDG
                     LARGYLGRPDLTAERFVPDPYPGDSPGGRLYLTGDRVRHLADGTLEFLGRFDTQVKVR
                     GFRVEPAEVEAHLAEHPAVRASAVLAHEEERGDRRLVAYVVPDPEYAAAGGDSGGEAA
                     ARQVEAWTRIFDDTYRADVPDATFNTVGWVSTATGLPLSREEMGEWAEDAVDRILALA
                     PRRVLEIGCGTGLLLFPIAPHCEQYTGTDISPRVLASLAEHLGDLGSRVRLLERPADR
                     FDGIEDESLDLVILNSVVQYFPDADYLATVLERAVAAVRPGGAVFLGDVRSLPLLDAF
                     YASVELAQAEPGCPASRIRRRAETRRLQENELVVAPAFFAELARRLPGICRVEVHPKR
                     GRAHNELTAFRYQVVLRVGEKPPSVEIPWRDWREGMTLESLRRLLAGPDTLETAPLGI
                     RNIPNARSASHGVAAALLLQEETLDAAGLLARAGEAVRGAVDPQDLWDLGRGLGWQVD
                     LGWADPGAEGRFEAVFRRPSPGEEPVSLLLPSPPAPPESRPWSAYTNDPLRGQLARRL
                     APELRAFLGTRLPDYMLPAAFVPLEAMPLTPNGKLDHRALARLGLAADLSQGADRYAA
                     PRTPTEERLAAIWADLLKVPRVGAEDDFFTLGGHSLLATQAVSRVRDAFGVELPLRAL
                     FEAPTVAGLAAVIDSAREADPGLALPPIERVPRDRPLPLSFAQQRLWFLDRLVPDNPF
                     YNMFSAVRLAGALDVAALRGACREITRRHEVLRTTFEGASGQPFQVIHPEPAAALTGI
                     LPLIDLGGLPEAARPAELERLAAAEGRRPFDLQGGPVFRNALVRLAEAEHSLFLNIHH
                     IAADGWSMGILHRELAALYEAFARSRSSPLPELPIQYADFAVWQRG"
BASE COUNT          590 a         1987 c         1800 g          768 t
ORIGIN      
        1 ccacccaggc gatgtcgcgg gtgcgccagg cgttcggcgt cgaggtgccc ctgcgtgccc
       61 tgttcgaggg gcccaccgtc gccgagctgg cgcgcgtcgt cgaagcgggc ctggctgacc
      121 tggcgacggc cgggccggag gcgccccccc tggtccccat gccggcggcg atgcgggcgg
      181 gcgagctgcc gctctccttc gcccagcagc gcctctggtt cctcgaccgc ctggtgccgg
      241 acaacccctt ctacaacatg ttcggcgcgg tgcggctcgc cggcccggtg gacgtgccgg
      301 ccctgcgcga ggcttgccgc gagatcgcgc gccgtcacga ggtgctacgc accactttcc
      361 cctcggtggc gggccggccg gtccaggcca tcgccgccga gccgcccccg gggttcccgc
      421 tgatcgaact gggcgggctg ccggaagcgc cgcggcggga ggagctggag cgcctcgcca
      481 acgccgaagg ccggcggccg ttcgacctga cgcgcgggcc ggtcttccgc gccgccctgc
      541 tgcggctggg cgaggccgag cactcgctct ttttcaacct ccaccacatc gtttcggatg
      601 gctggtcgct gggcgtcctc caccgcgatc tcgccgtgct ctacggcgcc ttccaccgtc
      661 ggcagctctc ccccctcccc gagctgccgg tccagtacgc cgacttcgcg gtctggcagc
      721 gcggctggct ccagggggag gcgctgcgcg cgcgtctgga ctactggcgg ggccagctcg
      781 agggcatgcc cgaggtgctg gagctgccca ccgaccggcc acggccggcc atcgaatcgt
      841 tccgcggcgc cggcgccccc ttcccgctgg cggcgccgct ggtgcgccgg ctgtcgctgc
      901 tcgcccgccg ccgtggcgcg acgctctcga tggtggtcct cgccggcttc aaggcgctcc
      961 tcgcccggct ctccggccgc ggcgacgtcc ccgtgggcgt ggccatcgcc ggccgcaacc
     1021 gccgcgagac cgaggagctg atcggcttct tcgtcaacac cctggtcctg cgcacccaag
     1081 tctccggcag cgccggcagc gtcggcgcgg ggggcttcgg cgacctggtc gcccgcgtgc
     1141 gggagacctc cctcggtgcc tacgcccacc aggacctccc cttcgagaag ctggtggagg
     1201 aactggcgcc ggaccgcgac ctcggccgca acccgctcgt ccaggtgacg ttcggcttcc
     1261 acaacttccc ccgccgggag gtcgagaccg gcgggctcgt gctgataccg ccgtccgagg
     1321 aggcggtcgg caccggcacc tccaagttcg acctgtcgct ccacctcttc gaggcggacg
     1381 gggcgctcca ggggacgctc gaatacaacc tcgacctgtt cgacgagccc acggcgcggc
     1441 gctttaccgg ccatctcgcg acgctgctcg cggcagccgt ggaggatccc gaggtggagc
     1501 cggccctgct gccgcttctc tccgaggccg agagccacca gctcctgacc gcctggaacg
     1561 acacggcctc cccgtacgag cgtgacctga cggtgcacga ggtcttcgat ctccaggtgg
     1621 aggcgaggcc ggacgccgtg gcggtgattg cgggagacga gcacctgacc tacctcggtc
     1681 tcgcccgccg cgcccgccgg ctggccgcgg acctccgcgc gctgggcgtc ggcccggagg
     1741 aggtcgtcgg gcttcgcgcc gagcgctcgg ccggtcttcc cgcggccatg ctgggcatcc
     1801 tctacgccgg cgccgcctat ctccccctcg atcccgaagc tcccgaagag cggctcgccc
     1861 tgatgcgcga ggaggcgggg gtccggttcg tcgtgtcagg gggggcggtg atgcctcccg
     1921 ctgctccagg tcaggagggg ggctctcctt cctccctcgc ctacgtcatc tacacctcgg
     1981 gctccaccgg acgtccgaaa ggcgtcgcgg tcccgcaccg cggcatcgtg cgcctcgtcc
     2041 ggcaggccgg ctacgcccgt ctcgacgcgg acgagaccct gctccaggtc gcgcccgtct
     2101 ccttcgacgc ctccaccttc gagatctggg gggcgctgct caacggcggc cggctggtga
     2161 tgatgccgcc ggagaagccg acgctggacg gcatcggcga tgccctggcc cggcatgggg
     2221 tgacgacgct ccacctcacg gccggcctgt tccacgccat ggtggaggag cgcctcggag
     2281 cgctcgcggg gttgcgccag ctcctcaccg gcggcgacgt gctctcggcg ccccacgtgc
     2341 ggcgggcggc cgcgagcctc ccgcattgcc aggtcatcgc ctgctacgga cccaccgagg
     2401 ggacgacctt cacctccgtc catctcatcg atccgaacgg agccgggcac ctcgatacct
     2461 ccgtccctct cggccggccg atcggcaaca cccgagtcct ggtgctggac cgctcgctcc
     2521 tgccggtccc cgtgggcgtg gcgggggacc tggcggtcgg cggtgacggc ctcgcccggg
     2581 gctacctcgg ccggccggac ctcaccgccg agcgcttcgt gccggatcct tacccaggcg
     2641 acagccctgg cgggcggctc tacctcaccg gcgaccgggt gcggcacctc gcggacggca
     2701 cgctggagtt cctggggagg ttcgacaccc aggtcaaggt gcgcggcttc cgggtcgagc
     2761 ccgccgaggt cgaagcccat ctggccgagc acccggcggt gcgggcgagc gcggtgctgg
     2821 cgcacgaaga ggagcggggg gaccgccgcc tcgtcgccta tgtcgtgccg gatccggagt
     2881 acgcggcggc cggcggcgat tccggaggtg aggcggcggc ccggcaggtc gaggcgtgga
     2941 cgcggatctt cgacgacacc taccgcgccg acgtccccga tgcgacgttc aacaccgtcg
     3001 gctgggtcag caccgcgacc gggttgccgc tctcccggga ggagatgggg gagtgggcgg
     3061 aggacgcggt ggaccgcatc ctcgctctcg cgccccgccg cgtcctcgag atcggctgcg
     3121 gcaccgggct cctgctgttc cccatcgcgc cgcactgcga gcagtacacc ggcaccgaca
     3181 tctccccccg cgtcctcgct tccctcgcgg agcatctggg ggacctgggc tcccgggtgc
     3241 ggctcctgga gcggccggcc gaccgcttcg acggcatcga ggatgagtca ctcgacctgg
     3301 tgatcctcaa ctccgtcgtc cagtacttcc ccgacgccga ctacctggcc accgtgctgg
     3361 agcgggcggt tgcggccgtg cgtcccggcg gcgcggtctt cctgggcgac gtgcgcagcc
     3421 tgccgctgct cgacgccttc tatgcctcgg tggagctggc gcaggcggag cccggctgcc
     3481 cagcgtcgcg gatacgccgg cgtgccgaga cgcggcgcct gcaggaaaac gagttggtcg
     3541 tcgctcccgc tttcttcgcc gagttggcgc ggcggctgcc cgggatctgc cgggtggagg
     3601 tccatccgaa gcgcggccgt gcgcacaacg agctgaccgc cttccgctat caggtcgtcc
     3661 tgcgggtagg ggagaagcct ccctccgtgg agatcccgtg gcgggattgg cgggagggga
     3721 tgaccctcga gtccctgcgc cgtctcctcg caggcccgga caccctagaa accgccccgc
     3781 tgggaatccg gaacatcccc aacgcccggt ccgcgtccca cggcgtcgcc gccgcgctcc
     3841 tcctccagga ggagaccctg gacgccgccg ggctgctggc gcgggccggc gaggcggtgc
     3901 gcggcgcggt cgatccccag gacctgtggg atctgggccg ggggctcggc tggcaggtag
     3961 acctgggctg ggccgatccc ggcgccgagg ggcgcttcga agcggtgttc cgccggccgt
     4021 ctcccgggga ggagccggtc tcgctgctgt tgccttcgcc cccggcccct ccggagagcc
     4081 gcccctggag cgcctacacc aacgacccgc tccggggcca gctcgcccgc cgcctcgccc
     4141 ccgagctgcg cgcctttctc ggcacccgcc tgcccgacta catgctgccg gcggccttcg
     4201 tgccgctcga ggcgatgccg ctcacgccga acggcaagct ggaccacagg gcgctcgccc
     4261 ggctcggcct cgccgcggac ctctcgcagg gtgccgaccg ctacgccgcg ccgcgcacgc
     4321 ccaccgagga gcggctggcc gcgatctggg cggacctcct taaggtgccg cgggtcggag
     4381 ccgaggacga cttcttcacg ctgggcggtc actcgctcct cgccacccag gcggtgtcgc
     4441 gggtacggga cgccttcggc gtcgagctgc cgctccgcgc cctgttcgag gcgccgaccg
     4501 tggccgggct cgcggcggtc atcgacagcg cccgcgaggc ggaccccggc ttggcgctgc
     4561 cgcccatcga aagggtgccg cgcgaccgcc cgctgccgct ctccttcgcc cagcagcggc
     4621 tctggttcct cgaccgcctg gtgccggaca accccttcta caacatgttc agcgccgtgc
     4681 gcctcgccgg cgccctggac gtggcggccc tgcgcggcgc ctgccgcgag atcacccgcc
     4741 gtcacgaagt gctgcgcacc accttcgagg gagcgtcggg ccagccgttc caggtcatcc
     4801 acccggagcc ggctgccgct ctgaccggta tcctgcccct gatcgatctc ggcgggctgc
     4861 cggaagcggc ccggccggcc gagctggagc ggcttgcggc cgccgagggg cggcgcccgt
     4921 tcgacctcca gggcggcccg gtcttccgca acgccctggt gcgcctggcc gaagccgagc
     4981 actcgctctt cctgaacatc catcacatcg ccgccgacgg ctggtccatg ggcatcctcc
     5041 atcgcgagct ggcggcactc tatgaggcgt tcgcccggag ccgctcttcg cctctccccg
     5101 agctgcccat ccagtacgcc gacttcgccg tctggcagcg cggct
//
LOCUS       DASPJI010000747         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_447353, whole genome shotgun sequence.
ACCESSION   DASPJI010000747 DASPJI010000000
VERSION     DASPJI010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447353"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VEL74_20910"
     CDS             <1..729
                     /locus_tag="VEL74_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HYG65056.1"
                     /translation="AGAPTRDKIRSTLGGPLDGMLAERVVLSEEGVVRTPEHLSDEEA
                     ATLPCAAVTAWNALVTEGRVAAGETVLVQGTGGVSLFALQLAKLLGARVIATSSSDDK
                     LARARELGADEGINYKDVPDWGARAKELTGGEGVDLVVEVGGAGTLQQSLRAVRIGGR
                     VALIGNLAGTSAEVPLTLIFMQRVNVYGILVGDRESFEAMNRAIAAHRLRPVVDRVFP
                     LDDTRAAFEHMAAGRHFGKIVIGL"
     gene            complement(961..>2724)
                     /locus_tag="VEL74_20915"
     CDS             complement(961..>2724)
                     /locus_tag="VEL74_20915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65057.1"
                     /translation="PDLTTNKKEWQQQAASGGLTPDVTGAENFTTIVAVAVSPVERGV
                     IWVGTDDGRVHVTRDGGTSWTSVEKALPGVPANTWVPHIRPSKFEGGGAFIVMEDHRR
                     SNWTPYVFRTDDYGKSWKSLATDEIRGWALSIEQDAIDPDLLFLGTEMGLYASFDGGK
                     SWLPWRHGVPTTPVADLVVHPRDHDLVVATHGRSIYIVDDVAPLRGMSAETLAKPVHL
                     YPSSGAVLHHRMPAGGASRPGAGEFRGENRPFGAILTYSLNAPGLPLPDDEKERARKE
                     AERTASRERALPRTSEPAAAAPLREDPPSRQEPGPTTGETADEAGKGKEPKVEIRITD
                     ASGKLVRKLEGPAKLGINRMVWDLSRDPFRQPPRGERGGWGEDGGPAVPPGTYNVTVK
                     HGQQEARGTVRVLPPPGSRMQEADWQAREAAIRRAGAARDALVEAIERIAGTRKDVDA
                     VLARLEQQDKERTRRGHTEGGAEADPHKELKQAARQLRRKLSDMEKRLFVPPDTKGIV
                     DEDAAVLSQVELAEFALTSAWSGPNPTQTAYLEQAEATVRAVLGDFNRLFAEDVAAFR
                     KQVQAAGIGWLPELAPVAVGR"
BASE COUNT          313 a         1019 c          976 g          416 t
ORIGIN      
        1 gccggcgcgc cgacccgcga caagatccgc tcgaccctcg gcggcccgct cgacggcatg
       61 ctcgccgagc gggtggtgct ctcggaggag ggggtggtgc ggacgcccga gcacctcagc
      121 gacgaggagg ccgccaccct gccgtgtgcc gcggtcaccg cctggaacgc gctggtgacc
      181 gaggggcggg tggcggcggg cgagaccgtc ctcgtccagg ggaccggcgg cgtctccctc
      241 ttcgccctgc agctcgccaa gctcctcggc gcgcgggtga tcgccacctc gagcagcgac
      301 gacaagctcg cccgggcgcg ggagctcggt gcggatgaag gcatcaatta caaggacgtg
      361 ccggactggg gtgcccgggc gaaggaactg acgggaggcg agggggtgga tctggtggtc
      421 gaggtcggcg gcgccgggac gctccagcag tcgctccggg ccgtgcggat cggcggccgg
      481 gtcgccctga tcggcaacct cgccggcacc tcggccgagg tccccctgac cctgatcttc
      541 atgcagcggg tgaacgtcta cggcatcctc gtcggggacc gcgagagctt cgaggcgatg
      601 aaccgcgcca tcgcggccca tcgcctccgc cccgtcgtcg accgcgtctt ccccctggat
      661 gacacccgcg ccgccttcga gcacatggcc gcggggcgtc acttcgggaa gatcgtgatc
      721 gggttgtagg ggggcctgcc tccaagggga cgtctacgcg cgagtcctca ccccccgacc
      781 ccctctccca tctcccgccc acccatgccc cgggagaggg ggagaccagc aattcatctc
      841 ttgtttttct ccccctctcc cggcgggggg cggttgggag atgggagagg gggccggggg
      901 gtgaggactc cacgggcagg acgccccgcc tgcctcaacg ctatccagcc cctgtgcccc
      961 ctacctcccc accgccaccg gcgccagctc cggcagccat ccgatgccgg ccgcctgtac
     1021 ctgcttgcgg aaggcggcca cgtcctcggc gaagaggcgg ttgaagtcgc cgaggacggc
     1081 gcggaccgtg gcctcggcct gctccaggta ggcggtctgc gtcgggttcg gcccgctcca
     1141 cgcggaggtg agggcgaact cggcgagctc cacctggctc aggacggccg cgtcctcgtc
     1201 gacgatcccc ttggtgtccg gcggcacgaa cagccgcttc tccatgtcgg agagcttgcg
     1261 gcggagctgc cgggcggcct gcttcagctc cttgtgggga tcggcctcgg cgcccccctc
     1321 ggtgtgcccg cgccgcgtcc gctccttgtc ctgctgctcg agccgggcga ggaccgcgtc
     1381 gacgtccttg cgcgtgccgg cgatccgctc gatggcctcc accagggcgt cccgggcggc
     1441 gccggcccgc cggatggcgg cctcgcgcgc ctgccagtcc gcctcctgca tccggctgcc
     1501 cggagggggg aggacccgca cggtgccccg ggcctcctgc tggccgtgct tcaccgtgac
     1561 gttgtaggtg ccgggcggca ccgccgggcc cccgtcctca ccccagccgc cgcgctcccc
     1621 ccgcggcggc tggcggaacg ggtcgcgcga gaggtcccag accatgcggt tgatccccag
     1681 cttcgccggc ccctcgagct tgcggaccag cttgcccgag gcgtccgtga tgcggatctc
     1741 gaccttgggc tccttcccct ttccggcctc gtccgccgtt tcccccgtgg tggggccggg
     1801 ctcctgccgg ctcggcgggt cctcccgtag cggcgccgcc gcggccggct cgctggtgcg
     1861 gggcagggcc cgctcccggc ttgccgtcct ctcggcctcc ttgcgcgccc gctccttctc
     1921 gtcgtcgggg agcggcagcc cgggggcgtt cagcgagtag gtgaggatgg cgccgaacgg
     1981 ccggttctcc ccccggaact cgccggcgcc gggccgcgag gcgccgcccg ccggcatccg
     2041 gtggtgcagc accgcgccgc tcgacggata gaggtggacc ggcttcgcca gcgtctcggc
     2101 cgacatcccg cgcagcggcg ccacgtcgtc gacgatgtag atggagcgcc cgtgcgtcgc
     2161 caccaccagg tcgtggtcgc gcgggtggac caccaggtcg gccaccggcg tcgtcggcac
     2221 gccgtgccgc cacggcagcc agctcttgcc gccgtcgaag gaggcgtaca ggcccatctc
     2281 ggtgcccagg aagaggaggt ccgggtcgat cgcgtcctgc tcgatcgaca gcgcccagcc
     2341 gcggatctca tcggttgcca aactcttcca gctcttgccg tagtcgtccg tgcggaacac
     2401 gtacggcgtc cagttggagc gccggtggtc ctccatgacg atgaaggcgc cgcccccctc
     2461 gaacttcgac ggccggatgt gcggcaccca ggtgttggcc ggcacgccgg ggagcgcctt
     2521 ctcgacgctc gtccagctcg tgccgccgtc gcgggtgacg tggacccgcc cgtcgtcggt
     2581 gcccacccag atcacgccgc gctcgaccgg gctcacggcg acggcgacga tggtggtgaa
     2641 gttctcggcg ccggtgacgt ccggggtgag gccgccgctc gccgcctgct gctgccactc
     2701 cttcttgttg gtggtgaggt cggg
//
LOCUS       DASPJI010000748        23492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_447433, whole genome shotgun sequence.
ACCESSION   DASPJI010000748 DASPJI010000000
VERSION     DASPJI010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 23492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23492)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23492
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447433"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VEL74_20920"
     CDS             complement(<1..958)
                     /locus_tag="VEL74_20920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HYG65058.1"
                     /translation="MAAQNDLPIAVLGAGAAGLVAAIFAAGRGGRVLLLERTRDGGRK
                     ILVSGGGRCNVLPSKIEPARFVTDSSPNTLRKILLSWPLAEQRRFFEEEAGLPLALEE
                     ETGKLFPVSNSARDVRDGLMALAVRRGVEVRFGAMVSGLVPPSSPGGTWTVRLDGGGG
                     EIAAASVIVATGGLSLPGTGSDGTGLELMSRLGHTIHPTYPALTPLTAAPPRHAPLAG
                     ISLSVTLRAPGPGGDFATRGGFLFTHRGYSGPSVLDISHLAVRSRLAGGSPQPLLVQW
                     TDLDEAGWDALLRESPGTVLGALRRNLPDRLAEVLITECGVDG"
     gene            complement(986..4525)
                     /locus_tag="VEL74_20925"
     CDS             complement(986..4525)
                     /locus_tag="VEL74_20925"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HYG65059.1"
                     /translation="MGRGPLHEMASKKREDPPTPPAREVGKWLDLEKLRGGSGEDEPA
                     PPAPDEEALPGPPSRDFGYNLEKLSASPGARKEKVKERSYEAPRRSSRRAGPAVQYAE
                     LHAASAFSFLNGASLPEDLVEQAASLGLPAVALVDANGVYGAPRFYQAAKQAGIKALV
                     GAEVVLDEEPPPHRRGGPGGHRGPLGLLPEPPPPSTGEKAEELGPADRTRVTVLVESR
                     TGYKNLCKLLSAAARGRPKGEARASWDLIAEHAAGLRCLTGGDEGPVAQALARGGADA
                     AKRQLDRLAGLFGGRVHVELQRHRLRDEEHRNRTLADLARSLRLPLLATNGVRYARQE
                     DKSLHDVLTAIRHHTPLDAAGALLAAHRERHFKSAAEMARLFDDLPEAVAGSMELAQR
                     LDFTLADLGYRFPDYPLPPGETPASFLRHITWNGAKARFRPLTAKAQAQIQKELDLIE
                     KLDLAGYFLIVWDIVRFCLEEGILAQGRGSAANSAVCYALSITAVDPVKMDLLFERFL
                     SEERGEWPDIDLDLPSGDQREKVIQHVYAKYGPHGAAMTANVITYRDRSATREVAKAF
                     GYSAEQIDKLAGQLGSWTYDVARGDEKSFEKELTEAGFDPAEPRIRRFMDLWTRIHNL
                     PRHLGQHSGGMVIAAGRLDEVVPLEPASMPGRVVVQWDKDDCADLGIIKVDLLGLGML
                     SALEEMVPTIRDREGVHVDLAHLPPDDPAVYKMLQQADTVGVFQVESRAQMASLPRNH
                     PERFYDLVIQVGIIRPGPIVGNLASPYFERRNKRQPVVYPHPSLEPILERTLGVPIFQ
                     EQLLRVAMVAAGFTGGEAEELRRAMGSKRSVERMKAIVDKLRRGMTERGITGKAQEEI
                     VQGITSFALYGFPESHAASFALIAYASAYLKAHHPTAFYLGLLNAWPMGFYHPATLVK
                     DAQRHGVEVRPVDVQTSGLRCRWEDREPGAVGATGALRLGFKFVRGLRAKAGRVIEHE
                     QARAPFADADDLVRRCGLHTDELQMLAGVGALSSLGLTRRAALWQVARLGRPAGPLLD
                     RLPDPEPSPLPEMTDAEETQADYAGTQLTLGPHPVAYLREALAKRGVVPAADLDSRRD
                     GERVRTAGAVIVRQRPGTAKGLLFLTLEDETGMSQAIVPPDLLQQHRKLIVGSPGLVV
                     EGILQKRDGTISVKGEKFWSLKEISAVPSHDFR"
     gene            complement(4565..4945)
                     /locus_tag="VEL74_20930"
     CDS             complement(4565..4945)
                     /locus_tag="VEL74_20930"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HYG65060.1"
                     /translation="MQANVGTTERWGSLLGGGALLAYAMRRGWRSPLSAALGLGAATL
                     MFRGATGHCPLYSALGRNTAESGPEAGETAGETIRSGGRTWPLPEGARRVRPEENLED
                     VVDEASRESFPASDPPGYTPSKAG"
     gene            5032..7482
                     /locus_tag="VEL74_20935"
     CDS             5032..7482
                     /locus_tag="VEL74_20935"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HYG65061.1"
                     /translation="MSLDWKLLLMLLGILGLMQAACWLLARGLGRRLERHVMALGVAL
                     PLVLLAPWLDRARLLLPGDVFLPIIPGAADVPGLPAGPAAHRHDLLNDTIYQLLPWEV
                     EVRRALGEARLPLWSDLLEGGSSPWINPQAGVMSPVFLLARALPIQHHLLAALALKLL
                     VAFEGAWLLCRLAGASRASSLLAAAGAALGGALLPWALFPLSAAAAWVPWLACGTIRL
                     FRGAGGREVAVTALLTAALLLSGHPEVAAVGGLFVALCGLALARRASFRRGLGAAALA
                     AVLGFGLAAPHLVPFALHLPQSQRAQEAAAWQMPPYRFLPSRPLSWFLPGFGQFMLAP
                     ISPRAFGIPFQGEFRGPFHWADADSGYTGLVAFAGSLAVLAARRRRAWPFLGFSLVSL
                     LLAAQFLPLAHLIQAVPHLRTMAYVRFLPVGCLGLCIAGALGIDALLRSHRRRRDLLA
                     AWVGLAVAAALSLARHADLYVAALWVLLAAGAALACWRPRLRAYGAALLGTVLLLDLG
                     PWARNHLPTCHPALFYPRTGFIAMMEREVAAGGPFRAAGEQQTLFPSLLPVYGVADVR
                     PHNPLAPMPYLHTLDAAFGFMPSMEDYFPAFGGVDHPFLDFLGVRVLASTIGHAPPRT
                     LERIDDGSFGVFRLYRNPDALPRWFLPSGVKVIAEHEVTRWIARLRNPRRVAVFDARA
                     KAWGGAAGDIGDVRLLASKPGQASLSVPGAGDRLLATSLPSPEGWRARAVGEPLETLV
                     VNGAFLGVRVPAGASRVELRYRPSGFVPGVLAGILSLFVTAAILARGLKLPRRSETRK
                     GEGDEDSTRPPVPAGRHL"
     gene            7436..9571
                     /gene="glgX"
                     /locus_tag="VEL74_20940"
     CDS             7436..9571
                     /gene="glgX"
                     /locus_tag="VEL74_20940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HYG65062.1"
                     /translation="MKIQPGRPYPLGATYDGAGTNFSIFSEVAERVELCLFDRDGVET
                     RVELPEMTAFCWHGYLPNVVPGQRYGFRVHGPWIPGEGHRCHPSKLLLDPYAKAIDGE
                     VKWDEAVFPYRFGDPDGSPNDADSAPFMPKSVVINPFFDWGGDHLLGTPMHNTVIYEV
                     HVKGFTMRHPDIPEELRGTYAGLAHPAAIRHLKKLGVTAVELLPVHQFIHDSLLAERG
                     LRNYWGYNSIGYLAPHNEYAAAGGSLGQQVQEFKQMVKLLHREGIEVILDVVYNHTAE
                     GNHMGPVLSFKGIDNGAYYRLMPDDRRFYMDYTGTGNSLNMRHPHVLQLIMDSLRYWV
                     LEMHVDGFRFDLAATLARELHDVDRLSAFFDLIQQDPVVSQVKLIAEPWDVGEGGYQV
                     GNFPPLWSEWNGKYRDTVRDYWRGEDQTLGEFAERFTGSSDLYALNGRRPYASINFVT
                     AHDGFTLRDLVSYNDKHNEANGEENRDGESHNRSWNGGAEGPTEDPEVNALRARQQRN
                     FLTTLILSQGIPMLLGGDEIGRTQGGNNNAYCQDNEISWLDWESVDQDLLGFTSALIA
                     FRREHPVFRRRRWFVGRSIRGSAARDIGWFRPDGREMGEEDWQRGFAKSVGVYLNGQA
                     IPSLDERGDRVAGASFYLVFNAHHEALEFQLPPADPWGKRWICVLDTGDPQHRPGERR
                     GGQRQENAYAAGEPVSVAGRAVVMLQRVE"
     gene            complement(9600..10229)
                     /locus_tag="VEL74_20945"
     CDS             complement(9600..10229)
                     /locus_tag="VEL74_20945"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HYG65063.1"
                     /translation="MQRPSGHAVPARSLRSSSLAGPDVRGWTVVAADGEPVGRVHDLL
                     VDEGSQKALFLDVEVAPALTAGHRRRDMGLPLNEANLPDPARAEPVHRLHDVNPGSGE
                     VYPTGEEPHPRVHSFESPEESLHHGDPGNHVLIPVGLALLKEDEDHVVLDSLRGTDVQ
                     DLPSYADGPVLPEMEMELRRHFQRGAAGELDLYDEERFFSARRRVESRR"
     gene            complement(10241..10483)
                     /locus_tag="VEL74_20950"
     CDS             complement(10241..10483)
                     /locus_tag="VEL74_20950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65064.1"
                     /translation="MAEIRVERKQRSVWPWLLGLVLLVLLAMGLMAMLGDDREPVGNN
                     TTEVGAMRQEQPVSPVPAYPTADLGEPLTPVVEAAA"
     gene            10699..12012
                     /locus_tag="VEL74_20955"
     CDS             10699..12012
                     /locus_tag="VEL74_20955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HYG65065.1"
                     /translation="MRSLFRMLFCALVLLLVPGIATAAETGAETGGPAPASAAAGPAF
                     DPVAATEAYLARMSPQQRARSDAYFEGGYWLQLWGLLYGLGVFALLLGTRLSARLRDA
                     VQRRIRSWPLQILLYAVGFILLVTLLSFPLSIYTDYVREHQYGMSNQTFGAWFGEELT
                     GLAVGMVLGGLSLLGLYTVLRKAPRTWWIWGTVVSVAFLIIGALIAPVFIAPLFNTYT
                     ELREEPLRTSILSLARANGIPAEHVYVVDASRQTKRISANVSGLGGTLRISLNDNLLR
                     RTSPAAVEAVMGHEMGHYVLNHIYELILEMGVVLFLGFAFLRWSFGKALARWGERWGV
                     RGVDDPAGLPLFAALFSLYFFIMTPVTNTIIRSNEAEADIYGINSSRQPDGFAEAALL
                     LGEYRKMAPGPLEEWIFFDHPSGRNRILMAMRWKAENAGKDGEPR"
     gene            12009..13862
                     /locus_tag="VEL74_20960"
     CDS             12009..13862
                     /locus_tag="VEL74_20960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65066.1"
                     /translation="MSDAPPGTGGGEGGLRNCDIVMKGGITSGVVYPSAVCELAKAFR
                     FRNIGGTSAGAIAAAATAAAEYGRREGKGTAFEGLAALPGRLAEKLPDGRSRLLSFFQ
                     PSTETKPLFEVFVTAVEGGGKARLIARVVRRVLAGFPVGGWIGALVVGLVPLLALVAA
                     WLAVDSLPLRLGLGLALLWQLVLIVAAAVAGSAAALIGRAARAIPENGYGLCRGYDAA
                     REGAHPPADPPPLTEWLADFLNELAGKPRRDEPLTFGDLESGAPGTSICLRMMTSSVT
                     LGRPFQMPFEKVFYFRESQLRDYFPAWVVKWMVDHPNPNAKTSPMLGGETLCPLPGPA
                     DLPVVVAARMSLSFPILISAVPLYAVDWRTPKESGPPEPETCWFSDGGLGSNLPIHFF
                     DAPLPRWPTVAINLAEKDHIQAREKDNVWLAKDNRGDILESWTRFDGLTGFVLSLVNV
                     MQNWRDETLAKMPGFRDRIAHIDLGPGEGGLNLVMGEEALKRLTERGTWAGIKLRRRF
                     TEPPGEEDKLSWENQRWVRYRSFMAAVEEMLGEFKAVYRQQDPGEPSYKDLIELRKPP
                     SYPLKSKKLQKRFLEMTERLMELADRLEAEPTFGEADPPSPSGDLRVVPRL"
     gene            13889..14302
                     /locus_tag="VEL74_20965"
     CDS             13889..14302
                     /locus_tag="VEL74_20965"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HYG65067.1"
                     /translation="MHEYRTRRRVEFVDTDLSGIAHFSRYLVFMENCEHEFLNALGLS
                     VFMEMDGQRIVWPRVAASCEYLSPARFEDVLEVHLKVLRKGGKSLTYGFEITREGTLV
                     ARGRTTAVCCVLDGSEVRSIPIPETIAGRIEEAPE"
     gene            14351..14683
                     /locus_tag="VEL74_20970"
     CDS             14351..14683
                     /locus_tag="VEL74_20970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011747863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HYG65068.1"
                     /translation="MAKNGIDLSKLSIPELQALAQEVQEEIVNRRETEKQRVLDQMRE
                     LAGSIGMSLEELILTQRGGRAAKTGAAVKYRHPENPELTWSGRGKRPQWLNDALAEGK
                     SLEDFAAE"
     gene            complement(14720..15400)
                     /locus_tag="VEL74_20975"
     CDS             complement(14720..15400)
                     /locus_tag="VEL74_20975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HYG65069.1"
                     /translation="MIASMKPSLVRRSLAEAVGTFALVVCGCGAIMVDAATGSLGHAA
                     VSASFGLVIMVMIAALGHVSGAHFNPAVTMAFAWIRHFPWRDVPLYIAGQLMGAVMGA
                     LTLRALFGTAGRLGATLPSGTSFQAFGVEILLTAVLMFVITSVATDTRAVGQLAALAI
                     GGTVALDALWGGPVTGASMNPARSFGPALVSGLWQAHWVYWLGPLLGATLGAALYQGL
                     RSETAPKD"
     gene            complement(15397..16161)
                     /locus_tag="VEL74_20980"
     CDS             complement(15397..16161)
                     /locus_tag="VEL74_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HYG65070.1"
                     /translation="MEAAAGFPPFLKLLADEQRWKLLEALAYSDRRVQELVRVLQQPQ
                     NLVSYHLRKLREHALVRERRSAADRRDVYYSLDLDRLKGMLMSTGEALHPGLGDEPDA
                     HRDVPLTREGKRVRVLFLCTHNSARSQMAEAILRQMAEDRVEVASAGTEVTRVHPLAA
                     KTMAARGIDSSGLRSKHLNEFLDQQFDYVITVCDRASETCPIFPGAPERIHWSIPDPS
                     SVEGDDATREAAFQRAADDLLTRIRYLLSLLQRRQG"
     gene            16350..16655
                     /locus_tag="VEL74_20985"
     CDS             16350..16655
                     /locus_tag="VEL74_20985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65071.1"
                     /translation="MSRRTLRRGAGALILAAGVLLPQASWGLDLQVPPGGRGALSALE
                     WTGWSDLWSFFTGALPKNRGSIDPDGEPVPSGSTQAAPAPTAPDPASENRGGIDPDG"
     gene            16783..17040
                     /locus_tag="VEL74_20990"
     CDS             16783..17040
                     /locus_tag="VEL74_20990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65072.1"
                     /translation="MCPASLPSSCDLRQILAEAEPEIGRILRRWRISEDEAEDLITCA
                     LIGVLLRWERIHNHREWVLRAIEKEARRRRTAHREEPDHGR"
     gene            17030..18226
                     /locus_tag="VEL74_20995"
     CDS             17030..18226
                     /locus_tag="VEL74_20995"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG65073.1"
                     /translation="MEDEKEAPAGYEQAIERAAARVGLHLDGLLREIDEAAARFSEIL
                     AAGEPERWLLVKTEPRFHGLKLCDLLLEKSREAWFTDPAGAVELAELAIAIAGRLDVE
                     RYGAAMVEEVRALAWAHLGNASRIASDLRRAEEALQTAESHLERSGGEALVEAQILSF
                     KASLRNSQGRFDEAAGLLDQALAIYRAAKDRHLEGKTLIKKGMTLGYSGRFMEAARLL
                     RRGLARIDPIEDPRLLVAARHNLIWYLDDSGQHREALSALEATRGLYLELGERMHLVR
                     LRWLEGKIARGLGRLDEAETALREARDAFAERGIGFDAALVSLDLAMVYVQRGETDEL
                     RRLATEMVPIFESRDVHQEALAALLLVHQAAETEQLSLGLLQRVASFLHRNRHDPGMR
                     FEGGEG"
     gene            complement(18247..18813)
                     /locus_tag="VEL74_21000"
     CDS             complement(18247..18813)
                     /locus_tag="VEL74_21000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65074.1"
                     /translation="MLEWADVWKILLGFASSLILIWIKELFVHYRQVVGLQKSLWSMM
                     EFESDLEEFLAALERMKASISEGNMRLVAFDVPKSITSAAEELAHLEPKNAYIYNDLQ
                     THAEIVRSGLTLLRELLKSLATMKPNPDDEIRLRKAIIGQVDILRSDRLSLGRAEVRV
                     MEHIKGCHPGLNKATVDKMNIALAKFNA"
     gene            complement(19170..20291)
                     /locus_tag="VEL74_21005"
     CDS             complement(19170..20291)
                     /locus_tag="VEL74_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYG65075.1"
                     /translation="MEKHPRGIKPESWLVSAGRASGPGSPLNVPLIPASNFIIGKGRE
                     YSRDDGTPTWEALEEVVGGLESGKAVAFASGMAAIASVFDQLAAGSVVVLPDDCYQGV
                     AGLVAAGAERQRWSVQRLAVDDTAGWIRSCGAADLIWLESPSNPLLTVADLEAVCAAP
                     RKPGAIVAVDNTFATPLNQQPLELGATVSLQSATKFIGGHSDLLAGIATTKDDALWHA
                     LRKSRELTGATPGTLEAFLAVRGARTLALRLQRAQQTALMLAERLERHPLITRVRYPG
                     LPSHPTHAIAKRVLKGFGTIISFELRGGAEFADGVCRNVSLIRHATSLGAVESTMERR
                     AAIPGQEHLPASLLRLSVGIEDADDLWADLESAISSAAR"
     gene            complement(20319..20882)
                     /locus_tag="VEL74_21010"
     CDS             complement(20319..20882)
                     /locus_tag="VEL74_21010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HYG65076.1"
                     /translation="MRSERDKMLAGELYDPFDPELVTARERARDLCLTLNGTRESQEA
                     ERRRILDDLFGAGGDTVWMQPPFFCDYGANIYLGTRVFFNFNCVVLDVCEVRIGDYTL
                     FGPGVQILTPLHPLDPELRRKQEFGKPIEIGSDVWVGGGALILAGVRIGSRAVIGAGS
                     IVTRDIPEGAFAAGNPCRVIRESASAV"
     gene            complement(21643..22545)
                     /locus_tag="VEL74_21015"
     CDS             complement(21643..22545)
                     /locus_tag="VEL74_21015"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG65077.1"
                     /translation="MSRLHRALPMMILAVLMAASAASAADPTPRDQAVAQAKKLMKQG
                     RHRDAAEAFRKAGKLGEGPCFDCLVGLAGALSGLGRHSEAVAAAREALQIPGIHPTRA
                     ALAYNELGAAQANESERNFDEAESNLRHAIDLGQDSVPVARYNLAELLRRRGRAAESA
                     KMARSYLAVDPMGQKAREARIVLCGVAAERGVPRPDTGQAIRMTNAKAADAPRRLYAP
                     SDVNLRSHATGLAGSKVRSHMIFELLVDQDGCVRRVEVLQEANKALAQSFAEAMRQWI
                     YLPKMVDGQPVAVLWQEKIKLDIG"
     gene            complement(22542..>23492)
                     /locus_tag="VEL74_21020"
     CDS             complement(22542..>23492)
                     /locus_tag="VEL74_21020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG65078.1"
                     /translation="VRLATMLVRVIGRDKKPIPDLRPEDFQVFVSGQEVPVYAVDWSP
                     AGSPSGTEPGKAETDTAQPEAPQAVIFVDAAIGHKTYVWIRDHLSLRPRLRELLASLP
                     PRTRIAVVALRSRLEIWQDFTQDREAVYQAITRALYLNGKPGPLTERSTDPASLTAHF
                     DFEAADRAIRHVPALTETVEALVPFSGVKSIFYLGANFDWHPWLYWKKKEASSIAEAL
                     RQARASVFVLDTRGSSYSRGVNNLPTLGRVGGGGIFYAGELGDLGLVAEVSSGYYILT
                     FDVDTLPRSGHFIVKLRGKKGEVLVPPVKIDLAFQKEESR"
BASE COUNT         3597 a         8016 c         8027 g         3852 t
ORIGIN      
        1 cgccgtccac cccgcattca gtgatcagca cctcggccag acggtccggc agattccggc
       61 gcagggctcc caggacggtg ccggggctct cccgcagcag cgcgtcccag ccggcctcgt
      121 ccagatccgt ccactggacc aggagaggct gaggcgatcc ccccgcgagg cgcgagcgta
      181 cggcgaggtg cgagatgtcc agcaccgacg ggccgctgta gccgcgatgg gtgaacagga
      241 agccgccgcg ggtggcgaag tctccaccgg gtccgggggc gcgcagcgtc acggagagag
      301 agatgccggc gagcggcgcg tgacgggggg gcgcggcggt caggggggtg agcgccggat
      361 aggtgggatg gatcgtatgc cccaggcggc tcatcagctc aaggccggtg ccgtcgctgc
      421 cggtgcccgg gagcgacagg ccgccggtgg cgacgatcac cgaggcggcg gcgatctccc
      481 cgccgccgcc gtcgagccgc acggtccagg tgccgccggg agaggagggg gggacgaggc
      541 ccgacaccat ggccccgaag cggacctcga cgccgcgccg gacggcgagg gccatgagcc
      601 cgtcccgcac gtcccgggcg ctgttggaga cggggaagag cttgccggtc tcctcttcca
      661 gggcgagggg cagccccgcc tcctcctcga agaagcggcg ctgctcggcg agcggccagg
      721 agaggaggat cttgcgcagg gtgttgggcg aggagtcggt gacgaagcgc gccggctcga
      781 tcttcgacgg caggacgttg caccgcccgc cgccgctcac caggatcttg cggccaccat
      841 cccgggtccg ctcgaggagc agcacccggc cgccccggcc cgcggcgaag atcgccgcca
      901 ccagccccgc cgccccggcg ccgagcacgg cgatggggag gtcgttctgg gccgccatat
      961 tcacgttcaa attagcacag cgacctcacc tgaagtcatg gctgggcacc gccgagatct
     1021 ccttgagcga ccagaacttc tctcccttga ccgagatcgt gccgtccctc ttctgaagga
     1081 tgccttcgac caccaggccg ggggagccga cgatgagctt gcggtgctgc tgcaggaggt
     1141 ccggcgggac gatcgcctgt gacatgccgg tctcgtcctc cagggtcagg aagaggagcc
     1201 ccttggccgt gccgggacgc tggcgcacga tcaccgcgcc tgccgtgcgg accctctcgc
     1261 cgtcccggcg gctgtccagg tcggcggcgg ggactacgcc gcgcttcgcc agcgcctccc
     1321 gcaggtaggc caccgggtgc ggccccaggg tgagctgggt gccggcgtag tccgcctggg
     1381 tctcctcggc gtcggtcatc tcggggagcg gcgacggctc cgggtcaggg agccggtcga
     1441 ggagcgggcc cgcgggccgg cccaggcgcg ccacctgcca gagggcggcg cggcgggtga
     1501 ggcccaggga cgagagggcg cccaccccgg cgagcatctg cagctcgtcg gtgtgcaggc
     1561 cgcagcggcg gaccaggtcg tcggcgtcgg cgaagggggc ccgcgcctgt tcgtgctcga
     1621 tcacccggcc cgccttggcg cgcagcccgc ggacgaactt gaagccgaga cgcaacgcgc
     1681 cggtggctcc aacggccccc ggctcccggt cctcccagcg gcaacgcaag cccgaggtct
     1741 ggacgtccac cggccgcacc tccacgccgt gccgctgcgc gtccttgacc agcgtcgccg
     1801 ggtggtagaa gcccatgggc caggcgttga ggagcccgag gtagaaggcg gtggggtggt
     1861 gggccttcag gtaggcgctg gcgtaggcga tcagggcgaa gctcgcggcg tgggactcgg
     1921 ggaagccgta gagggcgaac gaggtgatcc cctgtacgat ctcctcctgc gccttgccgg
     1981 tgatcccccg ctcggtcatg ccgcgacgca gcttgtccac gatcgccttc atccgctcca
     2041 ccgaccgctt ggagcccatg gcccgccgca gctcctcggc ctcgccgccg gtgaagcccg
     2101 ccgccaccat tgccacccgc aggagctgct cctggaagat cggcaccccc agcgtccgct
     2161 ccaggatcgg ctccaggctc ggatgcggat agaccaccgg ctgccgcttg ttccggcgtt
     2221 cgaagtaggg gctcgcgagg ttgcccacga tggggccggg acggatgatc cccacctgga
     2281 tcaccaggtc gtagaagcgc tccgggtggt tgcgggggag cgatgccatc tgcgcccggc
     2341 tctccacctg gaagacgccg acggtatccg cctgctgcag catcttgtag accgccgggt
     2401 cgtccggcgg cagatgcgcg aggtccacgt gcaccccctc gcggtcgcgg atggtcggca
     2461 ccatctcctc cagcgccgag agcatcccga gccccaggag gtccaccttg atgatcccca
     2521 agtcggcaca gtcgtccttg tcccactgca ccaccacgcg gccaggcatg gacgccggct
     2581 ccagcgggac cacctcgtcc aggcggccgg cggcgatgac catgcctccc gagtgctgcc
     2641 ccaggtggcg gggcaggttg tggatgcgtg tccagagatc catgaagcgg cggatgcgcg
     2701 gctccgcggg gtcgaagccg gcctcggtca gctctttctc gaacgacttc tcgtcccccc
     2761 gggcgacgtc gtaggtccac gagccgagct ggccggcgag cttgtcgatc tgctccgccg
     2821 agtagccgaa ggccttggcc acctcgcgcg tcgccgagcg gtcgcggtag gtgatgacgt
     2881 tggcggtcat cgccgcgccg tgggggccgt acttggcgta gacgtgctgg atcaccttct
     2941 cccgctggtc gcccgagggg aggtcgaggt cgatgtccgg ccactcgccg cgctcttcgg
     3001 agaggaagcg ctcgaagagg aggtccatct tcaccgggtc gaccgcggtg atcgagaggg
     3061 cgtagcagac cgcgctgttg gccgccgagc cgcgcccctg ggcgaggatc ccctcctcca
     3121 ggcagaagcg gacgatgtcc cagacgatga ggaagtagcc ggcgaggtcc agcttctcga
     3181 tgaggtcgag ctccttctgg atctgcgcct gggccttggc cgtgagcggc cggaagcgcg
     3241 ccttggcgcc gttccaggtg atgtggcgga ggaaggaggc cggcgtctcg ccgggcggca
     3301 gcgggtagtc cgggaagcgg taccccaggt cggcgagggt gaagtccagg cgctgggcca
     3361 gctccatgga gccggccacg gcctcgggca ggtcgtcgaa gagccgcgcc atctcggcgg
     3421 cgctcttgaa gtgccgctcc cgatgggcgg cgaggagggc cccggccgcg tcgagcggcg
     3481 tatggtggcg gatcgccgtc agcacgtcgt ggaggctctt gtcctcctgg cgcgcgtagc
     3541 gcacgccgtt ggtggcgagg agcggcaggc gcagggagcg ggcgaggtcg gccagggtgc
     3601 ggttgcggtg ctcctcgtcg cgcagccggt ggcgctggag ctccacgtgc acccggccgc
     3661 cgaagaggcc ggcgagccgg tcgagctgcc gcttggcggc gtcggccccg ccgcgggcca
     3721 gggcctgggc gaccggaccc tcgtctcccc cggtgaggca gcggaggccg gcggcgtgct
     3781 cggcgatcag atcccaggac gcccgggcct cccccttggg ccggccgcgg gccgccgcgc
     3841 tcagcagctt gcagaggttc ttgtagccgg tgcggctctc caccaggacc gtcacccgcg
     3901 tccggtcggc ggggcccagc tcctcggctt tctcaccggt ggacggaggc ggcggctcgg
     3961 ggaggagccc caggggaccg cggtgacctc cggggccgcc gcgccggtgc ggcggcggct
     4021 cctcgtcgag caccacctcg gcccccacca gcgccttgat ccccgcctgc ttcgccgcct
     4081 gatagaagcg cggcgccccg tagacgccgt tggcgtccac cagcgccacc gccggcaatc
     4141 ccagggacgc cgcctgctcc accaggtcct cgggcaggga ggcgccgttc aggaacgaga
     4201 aggccgaggc cgcgtggagc tcggcgtact ggaccgcggg gcctgcccgg cgggaggagc
     4261 ggcggggggc ttcataggag cgctccttga ccttctcctt gcgcgcgccc ggggaggccg
     4321 agagcttctc caggttgtac ccgaagtccc gggacggagg gccgggcagg gcctcttcgt
     4381 ccggcgccgg cggtgcgggc tcatcctccc cggagccgcc gcgcagcttc tcgaggtcca
     4441 gccacttgcc cacctcccgc gccgggggag tgggcggatc ctctctcttc ttgctcgcca
     4501 tctcgtgcag gggcccccgc cccatgcacc gagcctaggc atcagaagag cgtaagtcaa
     4561 tccttcagcc ggccttcgaa ggggtgtagc ccggcggatc gctcgccggg aacgactccc
     4621 gggaggcctc gtccacgacg tcttcgaggt tctcctcggg ccggacgcgc cgggcgcctt
     4681 ccggcagcgg ccaggtgcgc cccccgctcc ggatcgtctc tccggctgtc tccccggcct
     4741 cggggccact ctcggcggtg tttcttccca gggcgctgta gagggggcag tgccccgtgg
     4801 cgccccggaa catcagggtc gccgctccga gccccagggc ggccgagaga ggtgagcgcc
     4861 agccgcggcg catcgcgtag gccagcaggg ctccgccacc cagcagcgag ccccagcgtt
     4921 cggtcgttcc aacgtttgcc tgcataatct ccctcctgtc ctctggtcaa ggcaaggctt
     4981 gtgccagaca ccgtcgtgac gggagcggcg cgagatccgt atacctgatt gatgagcctc
     5041 gactggaagc tccttctgat gctcctgggc atcctgggac tgatgcaggc cgcctgctgg
     5101 ctgctggccc gggggctcgg tcggcggctc gagcgccacg tcatggccct gggagtggcg
     5161 ctgcccctcg tgctcctggc cccctggctc gacagggccc gcctcctgct cccaggtgac
     5221 gtcttcctgc cgatcatccc gggagccgcg gacgtcccgg ggctgccggc gggccccgcc
     5281 gcgcaccgcc atgacctcct gaacgacacg atctatcagc tcctcccctg ggaagtggag
     5341 gtgcggcgag cgctcggcga ggcacggctg ccgctctggt ccgacctgct ggaaggcggc
     5401 agctcgccct ggatcaatcc ccaggccggg gtgatgtctc ccgttttcct cctcgcccgc
     5461 gccctgccga tccagcacca cctgctggcg gcgctggcgt tgaagctcct cgtcgcgttc
     5521 gagggcgcct ggctcctttg ccggctggcc ggagcgagcc gcgcgtcgag cctcctcgcc
     5581 gcggccggcg ccgcgctcgg cggggcgctc ctcccctggg ccctctttcc cctcagcgcc
     5641 gcggcggcct gggtaccgtg gctggcctgc ggcacgatcc ggctgttccg cggagccggt
     5701 ggccgggagg tggcggtcac cgctctcctc accgcggcgc tccttctttc cggacatccg
     5761 gaggtcgccg cggtgggagg tctcttcgtg gcgctctgcg gcctcgcgct ggcgcggcgg
     5821 gcctccttcc gccgcggcct cggcgccgcc gccctggcag ccgtcctcgg attcggcctg
     5881 gcggcgccac acctcgtccc cttcgccctg cacctgcccc agtcccagcg ggcgcaggag
     5941 gccgcggcct ggcagatgcc tccctatcgc ttcctcccgt cgcgtcccct ctcctggttc
     6001 ctgcccgggt tcggccagtt catgctggca ccgatcagcc cgcgcgcctt cggcatccct
     6061 ttccagggtg agttccgggg acctttccac tgggcggacg cggacagcgg ctacaccggc
     6121 ctggtggcgt ttgccggctc tctggcggtc ctcgccgcgc gtcgccgccg cgcctggccc
     6181 ttcctcggct tctccctggt ctccctgctg ctggccgcac agttcctgcc gctggcgcac
     6241 ctcatccagg ccgtgccgca tctacggacg atggcctacg tccgcttcct cccggtcggc
     6301 tgtctcgggc tctgtatcgc cggagccctg gggatcgacg ccctgctccg ctcccatcga
     6361 aggcgccggg atctcctcgc ggcctgggtg gggctcgccg tcgccgcggc cctgagcctg
     6421 gcgcggcacg cggacctcta cgtcgccgcc ctgtgggtgc tgctggcagc cggcgccgct
     6481 ctggcctgct ggcggccacg gctccgggcc tatggggcag cgctcttggg gaccgtgctc
     6541 cttctggacc tcgggccgtg ggcgaggaat catcttccca cctgccaccc cgcgcttttc
     6601 tatccccgga ccggcttcat cgcgatgatg gagcgggagg tggcagccgg cggacccttc
     6661 cgggcggcgg gcgaacagca gacgctcttc ccgtcgctcc ttccggtcta tggcgtcgcc
     6721 gacgtccggc cgcacaaccc gctggcgccc atgccgtacc tgcacaccct cgacgcggcc
     6781 ttcgggttca tgccctcgat ggaggattac ttccctgcgt tcggcggcgt tgaccatccg
     6841 ttcctcgact tcctcggcgt gcgggtcctg gcttccacca tcggccacgc gccgccgcgg
     6901 acgctggagc ggatcgacga cggcagcttc ggcgtgttcc ggctctaccg gaaccccgac
     6961 gccctgccgc gctggttcct gccgtcggga gtgaaggtga tcgcggagca cgaggtgaca
     7021 cgttggatcg ccaggctccg gaacccccgg cgggtggcgg tcttcgacgc gcgggccaag
     7081 gcctgggggg gcgctgctgg agacatcggg gacgtccggc tgctcgcctc gaagccggga
     7141 caggcctccc tttcggtccc cggcgcggga gaccggctcc tcgccacctc gctcccctca
     7201 ccggagggct ggcgggccag agccgttggg gagccgctgg agacgctggt ggtcaacggc
     7261 gctttcctgg gggtgcgcgt ccccgccgga gcctcccggg tcgagctgcg ctaccggcca
     7321 tcggggttcg tccccggtgt cctcgccgga atcctctccc tgttcgtgac cgccgccatc
     7381 ctggcacgcg gcttgaagct tccccggcgg tccgaaacga ggaagggaga gggagatgaa
     7441 gattcaacca ggccgcccgt acccgctggg cgccacctat gacggtgcgg gaaccaactt
     7501 ttccattttc tccgaggtag ccgagcgcgt cgagctctgt ctgttcgacc gcgacggggt
     7561 ggagacccgc gtcgagctgc cggagatgac cgcgttctgc tggcacggct acctgccgaa
     7621 cgtggtgccg ggccagcgct acggcttccg cgtccacggg ccctggatcc cgggggaggg
     7681 gcaccgctgc cacccctcca agctcctgct cgatccctac gccaaggcca tcgacggaga
     7741 ggtgaagtgg gacgaggcgg tcttcccgta ccgcttcggc gatcccgacg gatcgcccaa
     7801 cgacgccgac agcgccccct tcatgccgaa gtcggtggtc atcaatccct ttttcgactg
     7861 gggcggcgat cacctgctgg gcacccccat gcacaacacg gtgatctacg aggtgcacgt
     7921 caaggggttc accatgcggc acccggacat cccggaggag ctgcggggca cctacgccgg
     7981 cctggcccac ccggcggcga tccgccatct caagaaactg ggggtcacgg cggtcgagct
     8041 gctgcccgtc caccagttca tccacgacag cctgctggcc gagcgcgggc tgcgcaacta
     8101 ctggggctac aactccatcg gctacctcgc gccgcacaac gagtacgccg ccgccggcgg
     8161 ctccctgggc cagcaggtgc aggagttcaa gcagatggtc aagctcctcc atcgggaggg
     8221 gatcgaggtg atcctcgacg tggtctacaa ccacacggcc gaggggaacc acatggggcc
     8281 ggtactctcc ttcaagggga tcgacaacgg cgcctactac cgtctgatgc cggacgaccg
     8341 ccgtttttac atggattaca cgggcaccgg caacagcctc aacatgcggc acccgcacgt
     8401 gctgcagctc atcatggact cgctgcgcta ctgggtgctg gagatgcacg tggacggctt
     8461 ccgtttcgac ctggcggcga ccctggcccg ggagctccac gacgtcgacc ggctgtcagc
     8521 tttcttcgac ctcatccagc aggacccggt ggtaagccag gtcaagctca tcgccgagcc
     8581 ctgggacgtg ggggaggggg gctatcaggt gggcaacttc ccgcccctct ggtccgagtg
     8641 gaacggcaag taccgggaca ccgtccgcga ctactggcgg ggggaggacc agaccctggg
     8701 tgagttcgcc gagcgcttca ccggcagctc cgacctctac gccctcaatg ggcggcgacc
     8761 gtacgccagc atcaacttcg tcaccgccca cgacggcttc acgctgcgcg acctggtctc
     8821 ctacaacgac aagcacaatg aggcgaacgg cgaggagaac cgcgacggcg agagccacaa
     8881 ccgctcctgg aacggtggcg ccgaggggcc gacggaggac ccggaggtca atgccctgcg
     8941 cgcccggcag cagcgcaatt tcctcaccac cctgatcctc tcccagggca tccccatgct
     9001 cctgggcggc gacgagatcg ggcgcaccca ggggggcaac aacaacgcct actgtcagga
     9061 caacgagatc tcctggctcg attgggagag cgtcgaccag gacctgctcg gcttcacctc
     9121 ggccctgatc gcgtttcgcc gcgagcaccc ggtcttccgt cgccgccgct ggttcgtggg
     9181 ccgctcgatc cgcggctcgg cggcgcggga catcggctgg ttccggcccg acggccggga
     9241 gatgggtgag gaggactggc agcgcggctt cgccaagtcc gtcggggtct atctgaatgg
     9301 ccaggccatc cccagcctgg acgagcgggg cgaccgggtg gccggcgcca gcttctatct
     9361 ggtcttcaac gcccaccacg aggcgctcga gttccagctc ccgccggccg atccctgggg
     9421 aaagcgctgg atctgcgtgc tggatacggg agatccgcaa caccggccgg gcgagcggcg
     9481 ggggggccag cgccaggaga acgcctacgc ggcaggcgag ccggtgagtg tcgccggccg
     9541 tgcggtggtg atgttgcagc gggtggagta gccgccaagc cgagagccgc cgccagtcgc
     9601 tagcggcggc tctccacccg ccgccgggcg ctgaagaagc gctcttcgtc gtagaggtcg
     9661 agctcgccgg cggcgccgcg ctggaagtgg cgccgcagct ccatctccat ctcgggcagc
     9721 accgggccat cggcgtacga cggcaggtcc tggacgtcgg tgccgcggag gctgtcgagg
     9781 acgacgtggt cctcgtcctc cttgagcagg gcgaggccca cggggatcag gacatggttg
     9841 cccggatctc catggtggag cgactcctca gggctctcaa aggagtgcac ccgcggatgc
     9901 ggctcttcgc ccgtggggta gacctcaccg cttccagggt tgacgtcgtg caggcggtgc
     9961 accggctcgg cgcgggccgg gtccggcaga ttggcttcgt tcagggggag ccccatgtcc
    10021 cggcggcggt ggcccgccgt cagggccgga gcgacctcga catccaggaa cagtgccttt
    10081 tgcgagcctt cgtccaccag caggtcgtgc acccggccga cgggctcgcc gtcggcggcc
    10141 acgacagtcc agccgcggac gtcagggcct gcgagagagg agctccgcag gctgcgcgcg
    10201 ggtaccgcgt ggccgctcgg tctctgcatc actccctccc tcaagccgcg gcttccacca
    10261 ccggagtgag gggctcgccg agatccgccg tgggatacgc cggcaccggc gaaaccggct
    10321 gctcctggcg catggcgccc acctcggtcg tattgttccc caccggctcc cggtcgtcgc
    10381 cgagcatcgc catgagaccc atggccaaca gcaccaacag gacgagcccg aggagccagg
    10441 gccaaacact acgctgtttc cgctcaactc tgatttcagc catgttgtgc ctccatcgct
    10501 gtgcgtttca ccggctccga gcgagctcaa ccaccgtttg gctgagtggg gcggggcggc
    10561 tacgccgccc ggtggctcgg gctccgatgt ctcgtcgtcg ccccacgcgg aaggggttct
    10621 gcaaagaccg ggcccactct ctccaaccca ccggccgtta tagactgagc accgctccat
    10681 gaaataggag gacacagggt gagatcactt ttccggatgc tcttctgcgc gctcgtcctg
    10741 ctgctcgtgc cggggatcgc cactgccgcc gagacgggcg ccgagaccgg ggggcctgct
    10801 ccggcgtctg ccgccgccgg ccccgctttc gatcctgtcg cggccaccga ggcctacctg
    10861 gcgcggatga gcccgcagca gcgggcccgc tccgacgcct acttcgaagg gggctactgg
    10921 ctgcagctct ggggtttgct ctacgggctc ggggtgttcg ccctgctgct ggggacccgc
    10981 ctctccgccc gcctgcggga cgcggtgcag cgccggatcc gctcctggcc gctccagatc
    11041 ctgctctacg cggtggggtt catcctgctc gtcaccctcc tctcgttccc gttgtcgatc
    11101 tatacggact acgtccgcga gcaccagtac gggatgtcca accagacgtt cggcgcctgg
    11161 ttcggcgagg agctgaccgg cctcgcggtg gggatggtcc tgggcgggct gtcgctcctc
    11221 ggcctctaca ccgtgctccg caaggccccc cgcacctggt ggatctgggg cacggtggtc
    11281 tcggtcgcgt tcctgatcat cggcgcgctc atcgccccgg tcttcatcgc gccgctgttc
    11341 aacacctaca ccgagctgcg ggaggagccg ctgcggacct ccatcctctc cctggcgcgg
    11401 gccaacggca tcccggccga gcacgtctac gtggtggacg cctcgcgcca gaccaaacgg
    11461 atcagcgcca acgtcagcgg cctcggcggc accctgcgta tctccctgaa tgacaacctg
    11521 ttgcggcgca cgtcgccggc ggcggtggaa gcggtcatgg ggcacgagat ggggcactac
    11581 gtcttgaatc acatctatga gctgatcctg gagatggggg tggtgctctt cctcggcttc
    11641 gccttcctgc gctggagctt cggcaaggcg ctggcgcgct ggggggagcg ctggggcgtg
    11701 cgcggcgtcg acgacccggc ggggctgccg ctcttcgcgg cgctgttctc cctctacttc
    11761 ttcatcatga ctccggtcac caacaccatc atccgctcca acgaagcgga ggcggacatc
    11821 tacggcatca actcgtcgcg ccagcccgac ggcttcgccg aggcggcgct gctgctgggc
    11881 gagtaccgca agatggcgcc gggccccctg gaggagtgga tcttcttcga tcatcccagc
    11941 ggccggaacc gtattctcat ggcgatgcgg tggaaggccg agaacgccgg gaaggatggc
    12001 gagccgcgat gagcgatgcc ccgccgggca ccggaggggg ggagggaggc ctgcgcaact
    12061 gcgacatcgt catgaagggg gggatcacca gcggcgtggt ctacccctcg gcggtgtgcg
    12121 aactggcgaa ggcgttccgg ttccgcaaca tcggcggcac ctcggccggc gccatcgccg
    12181 ccgcggccac ggcggcggcc gagtacggcc ggcgggaggg gaaggggacg gccttcgagg
    12241 gcttggccgc gctaccgggg cggctcgccg aaaagctgcc ggatggcaga tcgcggttgc
    12301 tgtcgttttt ccagccgagc accgaaacga agccgctgtt cgaagtcttc gtcaccgccg
    12361 tcgaaggagg aggcaaggcc cggctgatcg cccgcgtcgt ccggcgggtc ctcgcggggt
    12421 tccccgtagg tggctggatc ggagccctcg tcgtcggcct cgtgccgctg ttggcgctcg
    12481 tcgctgcgtg gcttgccgtg gattcactgc ccctgcgcct gggattggga ctggccctcc
    12541 tctggcaact ggtccttatc gtggcggcgg cggtggccgg gagcgccgcc gcgctgattg
    12601 gtcgcgcggc gcgggcgatc cccgaaaatg gttatggttt gtgccgcggc tatgacgccg
    12661 cgcgggaggg ggcgcaccct cccgccgatc cgccgccgct gaccgaatgg ctggccgatt
    12721 tcctcaacga gctggcgggc aaaccccgga gagacgagcc gctgaccttc ggcgacctcg
    12781 aaagcggagc gccgggcacc tccatctgcc tgcgcatgat gacctccagc gtcaccctcg
    12841 gccgcccctt ccagatgccc ttcgaaaagg ttttctactt ccgggagtca cagctccgcg
    12901 actattttcc ggcgtgggtg gtgaaatgga tggtggatca tcccaacccc aacgccaaga
    12961 cctcgcccat gctcggcggg gagacgctat gccccctgcc ggggcccgcg gacctgccgg
    13021 tggtcgtggc ggcgcggatg agcctcagct ttcccatcct gatcagcgcc gtgccgctct
    13081 acgcggtgga ctggcggacg ccgaaggagt ccgggcctcc cgagccggag acctgctggt
    13141 tctccgacgg tggcctgggc agcaacctgc ccatccactt cttcgacgcg ccgctcccgc
    13201 gctggccgac ggtggcgatc aacctcgccg agaaagacca cattcaggcc agggagaagg
    13261 acaacgtctg gctggccaag gacaaccggg gcgacatcct cgagtcgtgg acccgcttcg
    13321 acggcctgac cggcttcgtc ctctcgctgg tcaacgtgat gcagaactgg cgggacgaga
    13381 cgctcgccaa gatgccgggc ttccgggacc gcatcgctca tatcgatctc ggaccggggg
    13441 agggggggct caatctggtg atgggggagg aggccctgaa gcggctgacg gagcgcggca
    13501 cctgggccgg gatcaagctg cgccggcgtt tcacggaacc gccgggcgaa gaggacaagc
    13561 tctcctggga gaaccagcgg tgggtacgct atcgctcctt catggcagcc gtggaagaga
    13621 tgctcggcga attcaaggcc gtctaccggc agcaggaccc cggagagccg tcctataagg
    13681 atctgatcga gctgaggaag ccgccctcgt accccctcaa gagcaagaag ctgcagaagc
    13741 ggttcctgga gatgacggag cgcctgatgg aactggccga ccgattggaa gccgagccga
    13801 ccttcggcga ggccgatccg ccaagccctt ccggcgacct gcgcgtcgtc ccccggctct
    13861 gaagctcctt ttcatacgga ggaaggacat gcacgagtac cgcacccgcc gccgggtgga
    13921 gttcgtcgac accgacctga gcgggatcgc tcacttttcc cgctatctgg tcttcatgga
    13981 gaactgcgag cacgagttcc tgaacgcgct ggggctgagt gtcttcatgg agatggacgg
    14041 ccaacggatc gtctggccgc gggtggcggc ctcctgcgag tacctctcgc cggcgcgctt
    14101 cgaggacgtg ctggaggtcc atctcaaagt ccttcgcaag gggggcaagt cgctcaccta
    14161 tggcttcgag atcacccgcg aggggaccct cgtcgcccgc ggccggacca cggcggtctg
    14221 ctgcgtcctc gacggctccg aggtgaggtc catccccatc ccggagacga tcgccggccg
    14281 gatcgaggag gcgccggagt agggcctcgg tatacttccc gactcgactt ctctttgagg
    14341 gagatcaccc atggcgaaga acggcatcga cctgtccaag ctgtccatcc cggaattgca
    14401 ggccctcgcc caggaggttc aggaggagat cgtgaaccgc cgggagacgg agaagcagcg
    14461 ggtgctcgac cagatgcgcg agctggcggg gagcatcggc atgagcctgg aggagctgat
    14521 cctcacccag cgcggcgggc gcgccgccaa gaccggagcc gcggtcaagt accgtcaccc
    14581 ggagaacccg gagctgacct ggtccgggcg cggcaagcgc ccacagtggt tgaacgacgc
    14641 cctggccgag ggtaagagcc tggaagactt cgccgccgag taggccctcg ccccccgggc
    14701 cccctgcgcg ccggcttctt caatccttcg gagcggtctc cgacctcagc ccctgataga
    14761 gggcagcgcc aagcgttgcg cccagcaggg ggcccagcca gtacacccaa tgggcctgcc
    14821 agaggccgga aaccagagcc ggaccaaagg agcgggcggg gttcatggaa gcacccgtca
    14881 caggtccgcc ccagagcgca tccagggcta ccgtcccgcc gatcgccagc gcggcgagct
    14941 ggcctacggc ccgcgtgtcc gtagcgaccg aggtgatcac gaacatgagc accgcggtca
    15001 gcaggatctc gactccgaac gcctggaacg aggtaccgga cggaagggtg gccccgagac
    15061 gtccagccgt cccgaagagc gcccggaggg tcaaggctcc catgaccgct cccatgagct
    15121 ggcccgcgat gtagagaggg acatcacgcc atgggaaatg ccggatccag gcgaaggcca
    15181 tggttacagc cgggttgaag tgggcccccg agacatgccc gagcgcggcg atcatgacca
    15241 tgatgaccag gccgaagctt gccgataccg cggcgtggcc cagagatccc gtcgccgcgt
    15301 cgaccatgat ggccccacag ccgcacacca cgagagcgaa cgtaccgacc gcttccgcca
    15361 acgatcggcg cacaagagaa ggtttcatgg aggcgatcac ccctgacggc gttgcagcag
    15421 ggagagcagg tagcggatac gagtcagcag gtcgtcggca gctctctgaa aggctgcttc
    15481 ccgcgtcgcg tcatccccct ccacgctgga aggatcggga atgctccaat ggatgcgctc
    15541 gggagcaccg gggaagatgg ggcaggtctc gctggcccgg tcacaaaccg tgatcacgta
    15601 gtcgaactgc tggtccagaa attcgttcag atgcttggat cgcaggccgc tgctatcgat
    15661 cccccgggct gccatggtct tggctgccag tggatgaacg cgggtcacct ctgtgccggc
    15721 gctggccact tcgaccctgt cctctgccat ctgccgcaag atcgcctctg ccatctggga
    15781 ccgagcgctg ttgtgggtac acaggaagag cacccggacc cgcttcccct cccttgtgag
    15841 cggtacgtcc cgatgggcgt cgggctcgtc tccgagcccc ggatggaggg cttcacctgt
    15901 gctcatgagc atccctttca aacggtccag atccaggctg tagtagacat cccgccgatc
    15961 ggcggcgctc cgccgctccc ggacgagggc atgctcccgg agcttccgga gatggtagga
    16021 gacgagattc tgcggttgct gcaggaccct gacgagctcc tggacccgcc tgtcgctgta
    16081 tgccagggct tccagcagct tccagcgctg ctcatcggca aggagcttga ggaacggggg
    16141 gaatcccgcg gccgcttcca tggtccatca atatagattg atgcaacctg ggttgcaagt
    16201 cctccggtat cgacgcgacg ttgcggcacg cggcccgccg ggagtttccc ggcgggccgt
    16261 ttctcgtcca tcgcactatt gtctccagag gcaggcacgc ccggccgctg gaggccgcgc
    16321 gctgacctgc tggaagaggg gtgaagtcca tgagccgcag aacccttcgt cgcggcgccg
    16381 gcgcgctgat cctcgccgcc ggcgtcctcc tccctcaggc ctcttggggg ctggatctcc
    16441 aggtgccgcc gggcggccgg ggagccctgt ccgccctcga atggaccggc tggtccgacc
    16501 tctggagctt cttcaccggc gctcttccca agaaccgggg cagcatcgac cctgacgggg
    16561 agccggtacc gtccgggagc acgcaggccg cgcccgcccc gaccgcaccc gatcccgcca
    16621 gcgagaaccg gggcggcatc gatccggacg gctagcggga ccacaaaggg gggctgcccg
    16681 gagccggagc ccccctcctt ctttgtctcg ggtgtcacgt tttagcctca ggcgggaaca
    16741 tatcttgttg gaagatgtct cagcgagcca gcggagaacg agatgtgccc tgcgtcccta
    16801 ccatcgtcct gcgacctccg gcagatcctc gccgaagccg agcccgagat cggccggatc
    16861 ctgcgccggt ggcggatctc cgaggacgag gcggaggatc tgatcacctg cgcgctcatc
    16921 ggcgtcttgt tgcgctggga acgtatccac aaccaccggg agtgggtgct gcgggccatc
    16981 gagaaggagg ctcgcaggcg tcgcaccgcg caccgggagg agccggacca tggaagatga
    17041 gaaggaagcg cccgcgggat acgaacaggc gatcgagcgg gccgccgcac gggtgggcct
    17101 tcacctggat ggcctgctgc gggagatcga cgaggcggcg gcccggttct ccgagatcct
    17161 cgctgccggg gaaccggagc gatggcttct cgtcaagacc gagccgcgct tccatggctt
    17221 gaagctgtgc gacctcctcc tggagaagag ccgcgaggcg tggttcaccg atcccgccgg
    17281 ggcggtggag ctcgctgagt tggcgatcgc catcgccggt cggctcgacg tggagcgcta
    17341 cggagccgcc atggtggagg aggtccgggc tctagcctgg gcccacctcg gcaacgcttc
    17401 ccggatcgct tccgacctgc gccgggccga ggaggccctg cagaccgccg aatcccacct
    17461 ggagcggagc gggggcgagg cgctcgtcga ggcacagatc ctcagcttca aggcatcgct
    17521 tcgcaattcc caggggaggt tcgacgaagc cgcgggactg ctcgaccaag cccttgccat
    17581 ctatcgcgcg gccaaggacc ggcatctgga gggaaagacc ctcatcaaga agggcatgac
    17641 gctcggctac agcggccggt tcatggaagc tgcgcggctg ctgcggcgcg gcctagcgcg
    17701 gatcgatccg attgaagatc cccgtctgct cgtggcggcc cggcacaacc ttatctggta
    17761 tctggacgat agcggtcaac atcgggaggc gctctccgca ctggaggcga cgcgggggct
    17821 gtatctcgag ctgggcgagc ggatgcatct ggtgcgtctg cgctggctgg aagggaagat
    17881 cgcccgcggg ctcggccgtc tggacgaagc cgagaccgcc ctgcgggagg cgcgggatgc
    17941 cttcgcggag cgcggcatcg gattcgacgc cgcgctggtc tcgctcgacc tcgccatggt
    18001 ctacgtccag cgaggcgaga ccgacgagct ccggcgcttg gcgaccgaga tggtcccgat
    18061 cttcgaatcc cgcgacgtcc accaggaggc cctcgccgcc ctcctcctcg tccaccaagc
    18121 cgccgagacc gaacagctca gcctcggcct cctccagcgc gtcgcctcct tcctccaccg
    18181 caaccgccac gaccccggaa tgcgtttcga gggcggggaa gggtgagagc agacagccgg
    18241 gaacaatcat gcattgaatt tggctagggc gatattcatc ttatcaacgg tggctttatt
    18301 gagtcccggg tggcacccct ttatatgttc catgactcgt acctcggccc tgcctaggct
    18361 tagtctgtcg ctcctcagaa tgtcgacctg tccaatgatg gcttttcgca agcggatctc
    18421 atcgtctgga ttcggcttca ttgttgcgag actctttagc agttctctca ggagtgtcag
    18481 gccgctcctg acgatctctg catgagtttg aaggtcattg tagatgtagg cattcttggg
    18541 ttccaggtgg gccagttcct ctgccgcgga ggttatgctc ttgggcacgt caaacgcgac
    18601 caacctcatg ttgccctcag aaatagacgc tttcattcgt tccagggcgg ccaggaactc
    18661 ctcaagatcc gattcgaatt ccatcatgct ccacaagctc ttctgcagtc ccactacctg
    18721 ccgatagtgg acaaagagct cctttatcca aatcaagatg agcgatgagg caaagccaag
    18781 tagaatcttc cagacgtctg cccattcaag catttagata cctcgccctg tcgcctagtg
    18841 ttgacggctt cgccgacggc acttaccgtc cgagagcagc ttattacctg tcgcctattt
    18901 gtacggatcc cgccatcggc agattgtcga gtcacgacct tcgagacctc gcaaggcttc
    18961 attgactcta tctccgtgag tcggggctgt caatgcgaca ggagtgacgt gtgggtgcct
    19021 gaatatggaa atgattccat ctgtttattg gtgtctgagg aacgggaggt ggctctcctg
    19081 cctctcgatc ccggagactg gcttgacctt gcggatcgcg ccaccccttc attgggcatg
    19141 gcaagatggt tgcgctgctg ccgacacagt caccgtgccg cagagcttat tgctgactca
    19201 agatcggccc agagatcgtc ggcgtcctcg atgccaacgc tgagccgtag gagcgaagca
    19261 gggaggtgtt cctgtcctgg gatcgcggct cttcgctcca tggtcgattc cacggctccg
    19321 aggctggtgg catggcggat gagactgacg ttccggcaga caccatcagc aaactcggcc
    19381 cctccgcgca gctcgaacga aatgatggtt ccgaagccct tgagcacgcg cttggcaatg
    19441 gcatgcgtcg ggtgtgaggg caagcctgga taccggacac gagtaatcag cgggtgcctc
    19501 tcgagccgct ccgcaagcat caaggccgtt tgttgggccc gttgcaagcg gagcgcgaga
    19561 gtcctcgcgc cccggacggc gagaaaggct tcgagcgttc ctggtgtcgc gccggtcaac
    19621 tcgcgagact tcctgagggc gtgccacagt gcatcgtctt tcgtcgtggc gatccctgcc
    19681 aacaggtcgg agtggccgcc gatgaacttg gttgccgatt gcagggacac cgtggcaccg
    19741 agctcgagcg gctgctgatt caaaggcgtg gcgaaggtgt tgtccacggc tacgatcgct
    19801 ccgggcttgc gtggtgccgc gcagacagcc tccaggtccg ctacggtcaa cagtgggttc
    19861 gagggtgatt cgagccatat caggtcggcg gcgccgcagg agcgaatcca gccggcagta
    19921 tcgtccactg ccaggcgctg caccgaccag cgttgtcttt cagcgcccgc tgcgaccaag
    19981 cccgccacgc cctgatagca atcatcggga agcaccacga cggaaccggc cgcaagctga
    20041 tcgaacaccg aggcaatggc ggccatgccg gaggcgaacg ccacggcctt gccagactcg
    20101 agcccgccga cgacctcttc gagtgcctcc caggtcggag taccatcatc gcgcgagtac
    20161 tcgcgcccct tgccgatgat gaagttcgat gccgggatca gcggtacgtt cagcggagac
    20221 ccgggcccgg aagcgcggcc tgccgagacg agccacgatt ccggtttgat tccacgcggg
    20281 tgcttttcca ttcttccgaa tacctccggc aaagagagct acaccgcaga cgccgattcc
    20341 ctgatgaccc ggcatgggtt gccggccgcg aaagcgccct cggggatgtc gcgcgtcacg
    20401 atgctcccgg cgccgatcac ggcgcgcgat ccgatgcgaa ccccggccag gatcagggcg
    20461 ccacccccca cccagacgtc tgagccgatc tctatgggtt ttccgaattc ctgcttcctg
    20521 cgcagctcgg gatcgagtgg atgcagcggt gtaaggatct gcacgccggg gccgaagagc
    20581 gtgtagtctc cgattcgcac ttcgcataca tcgagcacga cgcaattgaa gttgaagaag
    20641 acgcgcgtac cgagatagat attggcgccg tagtcgcaga agaacggcgg ctgcatccag
    20701 acggtgtcgc cgcccgcgcc aaagagatcg tcgaggattc gccgtcgctc cgcctcttgt
    20761 gattcacgcg tgccgttcag tgtgaggcac aggtcgcgtg cccgttcccg tgctgtcacc
    20821 agctccggat cgaacggatc gtagagctcg cctgccaaca tcttgtcgcg ttcgcttcgc
    20881 atcggatccc cccaacgccg ccgtggctcc tatcgttgac agctcgatct tgcaggagcg
    20941 taagccgacg gtcacgtgta gcgcgctcat ggcagcatcc gaggtgcgaa aggcgtgccg
    21001 gagctgccgg ttcggcgtcg gaggccggaa agctccgccg gatgctccgc cggcgatccc
    21061 cctcgagagg aggaccatcc tcgcggcgaa gagcaccata ccccgggtag gagcgccgta
    21121 cccggaggga gagcgagcgt acctccggta tggcggccat acgcggaggg atgacggtcg
    21181 tgcccactgg aagcgtgccg tccccacggg taggtcccct tccccgcggg tacggcgccc
    21241 atccctcgag ggatgacggc cgtcccgggt cgggcaatcg tcgagcggcc cattccaccg
    21301 gggtttcggg aggcggagac gttcctccgc ctcccactac gggggcgcgg cgcttcaggg
    21361 gcgtggcctt ggacccggcg gcgcccctgc acgattcagt tgtcaaagaa cttggccgcc
    21421 aaatcccagg ttgaagttgg gaaacgactg cacggctaca ttgtacgcgg atagcgactg
    21481 catcgccaga ggagagttga catcgtgggc tggggcgctg acaatagagg cagcaggggg
    21541 gagtgacgag accccgcggg aaggttcttt gacaatcgat ggaccagatc acgggcacgg
    21601 cgagcgcggc gccgtgcccg tggactgggg tgtcgctcgg ccctacccga tgtcgagctt
    21661 gatcttctcc tgccagagga cggccacggg ctgcccgtcg accatcttcg gcagatagat
    21721 ccattgacgc atcgcctcgg cgaagctctg cgcgagggcc ttgttcgcct cctggagcac
    21781 ttcgacccgg cggacgcatc cgtcctggtc gacgaggagc tcgaagatca tgtgcgaccg
    21841 gaccttcgag ccggccaagc cagtagcgtg ggacctcagg ttcacatcgc tgggcgcgta
    21901 gagccgccga ggggcgtccg cagccttggc gttcgtcatg cgaatcgcct gtcccgtgtc
    21961 cggccgcggg acgccgcgct ccgcggcgac gccgcagagg acaatccggg cctcgcgggc
    22021 cttctgcccc atgggatcga ccgcgagata agaccgcgcc atcttcgccg attcggccgc
    22081 acggccacgg cggcggagca gctccgccag gttgtagcgg gccacgggca ccgagtcctg
    22141 tcccaggtcg atcgcatggc ggaggttgga ctcggcctcg tcgaagttgc gctcggactc
    22201 gttcgcctgc gcggcgccga gctcgttgta ggccagggcg gcccgggtgg gatggatccc
    22261 tgggatctgc aaggcctcgc gagcggcggc gaccgcttcg ctgtggcggc ccagcccgga
    22321 gagcgcgccg gcgagcccga cgaggcagtc gaagcagggg ccctcgccca gcttgccggc
    22381 cttgcggaac gcctcggccg cgtcgcggtg gcggccctgc ttcatcagct ttttggcctg
    22441 ggcgacggcc tggtcgcgag gcgtcgggtc cgcggcgctc gcggcggacg ccgccatcag
    22501 gacggcaagg atcatcatcg gcagagcacg gtgcaatcga ctcatctcga ctcctccttc
    22561 tgaaaggcta gatcgatctt gaccggcggg acgagcacct cgcctttctt gccacgcagc
    22621 ttgacgatga agtgtccgga ccggggcagg gtatccacgt cgaacgtcag gatgtagtaa
    22681 ccggaggaca cctcggccac gagaccgaga tcgcccaact ccccagcgta gaagatgccg
    22741 ccgccgccga cgcggccgag ggtcgggagg ttgttgaccc ctctggagta gctgctccct
    22801 cgggtgtcga ggacgaatac cgaggcacgc gcctgccgga gagcctcggc gatcgaggag
    22861 gcctccttct ttttccagta cagccacgga tgccaatcga agtttgcccc caggtagaaa
    22921 atcgatttga cgccggagaa aggcacgagg gcttcgacgg tttcggtcag ggccggtaca
    22981 tggcggatcg cccggtccgc tgcctcgaag tcgaagtgcg ccgtgagcga tgcggggtcg
    23041 gtgctcctct ccgtcaaggg cccgggtttg ccgttgaggt acagcgcacg ggtgatcgcc
    23101 tggtagacgg cctcgcggtc ctgagtgaag tcttgccaga tctcgagacg ggagcggagc
    23161 gcaaccacgg cgatccgtgt gcggggaggg agcgaggcga ggagctcgcg cagccgtgga
    23221 cggagcgaga ggtggtcacg gatccagacg taggttttgt ggccgatggc ggcatccacg
    23281 aagatgacgg cctgaggagc ctccggctgg gctgtatccg tctccgcctt cccgggctcc
    23341 gtgccggagg gtgaaccggc gggggaccag tccaccgcgt agaccgggac ctcctgtccg
    23401 ctcacgaaaa cctggaagtc ttcgggcctg aggtccggga tcggcttctt gtcccgcccg
    23461 atcacccgga cgagcatggt ggcgagcctc ac
//
LOCUS       DASPJI010000749         5610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_504489, whole genome shotgun sequence.
ACCESSION   DASPJI010000749 DASPJI010000000
VERSION     DASPJI010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504489"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VEL74_21025"
     CDS             <1..535
                     /locus_tag="VEL74_21025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65079.1"
                     /translation="GDDPVYPRLSDLHMLLPAITGKVEMVYEGEQQGAEVVARRLIGQ
                     AVKKAFDATFPEVGKETGSGGEDDRGPYARIIRWFAEGNSVTLSDEQSFADYEAELFK
                     VASLSELVARQGKTREEKALAAEMILEGLHQHLKLARQDLDSQISYKEMVKFQLLKPR
                     RGSGRGDRSDRDRGDVN"
     gene            540..1628
                     /locus_tag="VEL74_21030"
     CDS             540..1628
                     /locus_tag="VEL74_21030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG65080.1"
                     /translation="MRHQYLYLDPALIQAILAGLDLLRLFNQLVLATGGDVDEAMEWM
                     RYLQDQGHLPSDLDLEAFFASLEDQQLVTRDSEGGVQLTAGGERRIRRSAFEEIFSTL
                     GRSGPGYHPIRASGEGVERQPETRPYKFGDEIHLLDSARSLQNAMKRTEGDLVLAEED
                     LEVYETEHLTSCATVVAIDISHSMILYGEDRITPAKTVALALTELITTKYPKDHLDVI
                     LFGDRAEQVFLSEIPYIQAGPYHTNTREALQLARGLLSRQKQPNKQIFLITDGKPSAI
                     TEGGHVYKNPFGLDMKIVNLTLEEADMCRRQKVVVTTFMLATDPMLTQFVEKLTQINR
                     GRAYFASPYNLGEFILADYIKNRRRRVR"
     gene            1641..2207
                     /locus_tag="VEL74_21035"
     CDS             1641..2207
                     /locus_tag="VEL74_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65081.1"
                     /translation="MPISRQSWMVLLILLAGVVALAGCGSGDEPEAAAGPDDSALQAC
                     ALFTQKDAEAAAGLTVSGTLSSTLNDVQGPNPLQCTFATGSLLEPGILSVEIRPARNA
                     KRAARLHEAGRGTIERLVQGEVEDVSGVGEGAYWAGGQLKQLHVVHGRYQLIITVQPG
                     KNQLAAAKQIASTVIARMRAQAPPAQTS"
     gene            complement(2219..3172)
                     /locus_tag="VEL74_21040"
     CDS             complement(2219..3172)
                     /locus_tag="VEL74_21040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65082.1"
                     /translation="MPGLQIPYQPLRTDLYSPDELLGGFDPSDPQSFARTLDFRIYQS
                     FHLEGGATPSDFFASMMRSLHDNSISQALAVLLAGKRCVAVMGGHRLRRDEPLYEEVA
                     VLSRGFTRRGFLVASGGGPGAMEATHLGAALANVPDGALADALARLATRPVLPAGLKD
                     LVGERGTVDPIVVAAAHAWYSPALEIWRGLADPGPSLAIPTWHYGHEPSSPLATHIAK
                     YFQNSIREDGLLAIATQGIVFAPGKAGTIQEIFQDATQNYYRTFQYFSPMVLLGREYW
                     TVTHPVDALLRSLFTPEDYARYVLVTDSIADALRFIEQFTP"
     gene            complement(3174..3890)
                     /locus_tag="VEL74_21045"
     CDS             complement(3174..3890)
                     /locus_tag="VEL74_21045"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HYG65083.1"
                     /translation="MIYLGVLILLSLLILIHEAGHLVAARLAGIPVSGFSVGFGPRVW
                     SRRWGATEYSLRALPLGGYVQPALDADGGDEGFRAIALRRRLVFFLGGPLANLAAALP
                     LFAAYNGMAGGRSFYALAVAPFAQVAGSCWHVLNLLPDLFRKPEAMSGVIGVVVDGGQ
                     LAASGGLVELALSLTISLAVLNLFPIPILDGGQIVMSCLEELFPRLARLRVPLTLAGL
                     VLLVGLMVYVNVQDVVRHWG"
     gene            4085..4978
                     /locus_tag="VEL74_21050"
     CDS             4085..4978
                     /locus_tag="VEL74_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG65084.1"
                     /translation="MLSISNLSKTYSNGVQALRDVSLDIPRGMFGLLGPNGAGKSTLM
                     RTIATLQDPDGGSITLDGLDVLRQKDEVRKVLGYLPQEFGIYPRISAEVMLDHFALLK
                     GITNDRERKEVVQALLHQTNLWDVRRRNLGTYSGGMRQRFGIAQALLGNPKLVIVDEP
                     TAGLDPQERNRFLNLLSEIGENVVIILSTHIVEDVSDLCSRMAIISQGRVLLTGEPQK
                     VIDTLRGRIWQKAIDKSELPDYQARYEVISTRLFAGRTVLHVHSLENPGTGFEPVDSD
                     LEDVYFHTLRQADPKVAAKAA"
     gene            5003..>5610
                     /locus_tag="VEL74_21055"
     CDS             5003..>5610
                     /locus_tag="VEL74_21055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYG65085.1"
                     /translation="MFKAISTFEVRYQLRQPLFYAVTLLLALMTFGAVTSDTVQIGGS
                     IGNVHRNAPLVIMRLLGAMTLIGMFVTTAFVASSAQRDFEHGTHSLFFSKPLNKRDYL
                     LGRFFGSVGISALVFLGPTLGILIGTFMPWLEPERLGPFTLAPYLYTFLIFVIPNLVF
                     TGAVFFTLASVTRSLLYTYLGVVVLFIGYIITQSMLRDVENV"
BASE COUNT          963 a         1990 c         1822 g          835 t
ORIGIN      
        1 cggcgacgac ccggtctacc ctcgcctctc cgaccttcac atgctcctgc cggcgatcac
       61 cggcaaggtg gagatggtct acgaggggga gcagcagggg gccgaggtgg tcgcccggcg
      121 gctcatcggc caggcggtca agaaggcgtt cgacgccacc ttccccgagg tgggcaagga
      181 gaccggcagc ggcggcgagg acgaccgggg cccctacgcg cggatcatcc gctggttcgc
      241 cgagggcaac agcgtcaccc tgtccgacga gcagtcgttc gccgactacg aggccgagct
      301 cttcaaggtg gcgagcctgt cggagctggt ggcgcggcag ggcaagaccc gcgaggagaa
      361 ggcgctggcc gccgagatga tcctggaagg gctccatcag cacctgaagc tcgcccgcca
      421 ggacctggac agccagatca gctacaagga gatggtcaag ttccagctct tgaaaccccg
      481 ccgcggctca ggccgtggcg accgcagtga ccgcgacagg ggagacgtga actaacccca
      541 tgcgccacca gtacctgtac ctcgaccccg ccctgatcca ggcgatcctg gcggggctcg
      601 acctgctccg cctcttcaac cagctcgtgc tcgccacggg cggtgacgtc gacgaggcga
      661 tggagtggat gcgctacctc caggatcagg ggcacctgcc gtccgacctc gacctcgaag
      721 ccttcttcgc cagcctcgag gaccagcagc tcgtgacgcg cgacagcgaa gggggcgtcc
      781 agctcacggc gggcggcgag cggcgcatcc gccgcagcgc cttcgaggag atcttctcca
      841 ccctcgggcg ctcgggcccg ggctatcacc cgatccgcgc ctccggcgaa ggggtcgagc
      901 gccagcccga gacccggccg tacaagttcg gcgacgagat ccacctgctc gactccgccc
      961 ggtcgctcca gaacgccatg aagcggaccg agggggacct ggtgctggcc gaggaggacc
     1021 tggaggtcta cgagaccgag cacctgacct cctgcgcgac ggtggtggcg atcgacatca
     1081 gccactccat gatcctctac ggcgaggacc ggatcacgcc ggcgaagact gtggcgctgg
     1141 cgctgaccga gctgatcacc accaagtacc ccaaggacca cctggacgtg atcctcttcg
     1201 gcgaccgcgc cgagcaggtc ttcctgtccg agatcccgta catccaggcg gggccgtacc
     1261 acaccaacac ccgcgaggcc ctgcagctcg cgcgcggcct gctgtcgcgg cagaagcagc
     1321 cgaacaagca gatcttcctg atcaccgacg gcaagccctc ggcgatcacg gaagggggcc
     1381 acgtctacaa gaaccccttc ggcctggaca tgaagatcgt caacctcacc ctggaggagg
     1441 cggacatgtg ccgccgccag aaggtggtgg tgacgacctt catgctggcc accgacccga
     1501 tgctcaccca gttcgtcgag aagctcaccc agatcaaccg cggccgggcc tacttcgcct
     1561 cgccgtacaa cctgggggag ttcatcctcg cggactacat caagaaccgc cgccggcggg
     1621 ttcgctgagc gagaaaaacc atgcccatct cccgacagtc gtggatggtc ctcctgatcc
     1681 tcctggccgg tgtcgtggcc ctcgcgggct gcggctccgg tgacgagccc gaggcggccg
     1741 caggcccgga cgactccgct ctccaggcct gcgcgctgtt cacgcagaag gacgcggagg
     1801 ccgccgcggg cctcaccgtc agcggcaccc tgagcagcac gctcaacgac gtccagggcc
     1861 cgaaccccct ccagtgcacc ttcgccaccg gctcgctgct ggagccgggc atcctcagtg
     1921 tcgagatccg gcccgcccgg aatgcgaagc gggcagcccg cctccacgag gcgggccgcg
     1981 gcaccatcga gcgcctcgtc cagggcgagg tggaggacgt ctccggggtc ggtgaagggg
     2041 cctactgggc gggtgggcag ctcaagcagc tccacgtcgt ccacggccgg taccagctca
     2101 tcatcacggt tcagccgggg aagaaccagc tcgcggcggc gaagcagatc gcctcgacgg
     2161 tgatcgcgcg catgcgggcg caggccccgc cggcccagac ctcctgagga ggctcgcctc
     2221 agggcgtgaa ctgctcgatg aagcgcaggg cgtcggcgat ggagtcggtc accagcacgt
     2281 agcgggcgta gtcctcgggc gtgaacagcg agcgcagcag ggcgtccacc ggatgggtga
     2341 cggtccagta ctcccggccc aggaggacca tggggctgaa gtactggaag gtgcggtagt
     2401 agttctgcgt cgcgtcctgg aagatctcct ggatggtgcc ggccttgccc ggcgcgaaga
     2461 cgatgccctg ggtggcgatg gcgagcaggc cgtcctcccg gatgctgttc tggaagtact
     2521 tggcgatgtg ggtggcgagc gggctggacg gctcgtgccc atagtgccag gtggggatgg
     2581 cgaggctcgg cccggggtcc gccaggccgc gccagatctc cagcgccggc gagtaccagg
     2641 cgtgcgccgc ggccaccacg atgggatcga ccgtccccct ctcccccacc aggtccttca
     2701 gccccgccgg caggaccggc cgggtggcga gccgggcgag ggcgtccgcc agggccccgt
     2761 cgggcacgtt ggccagggcc gctcccaggt gcgtggcctc catcgccccc ggcccgccgc
     2821 cgctcgccac caggaaaccc cggcgcgtga agccgcggga gaggaccgcg acctcctcgt
     2881 agagcggctc gtcgcggcgc agccggtggc cgcccatgac cgccacgcac cgcttgcccg
     2941 ccagcaggac ggccagcgcc tggctgatcg agttgtcgtg gaggctccgc atcatgctgg
     3001 cgaagaaatc gcttggcgtc gcccctcctt ccagatggaa cgactggtaa atccggaagt
     3061 ccagcgtccg cgcgaagctc tgggggtccg agggatcgaa cccgccgagc agctcgtcgg
     3121 ggctatagag gtcggtgcga agcggctgat aagggatctg gagtccgggc atcctagccc
     3181 caatggcgca ctacgtcctg cacgttgacg tagaccatca gccccaccag cagcaccagg
     3241 ccggccaggg ttagcggcac ccgcagccgc gccaggcggg ggaacagctc ctccaggcag
     3301 gacatgacga tctggccgcc gtcgaggatc gggatgggga acaggttgag caccgccagg
     3361 gagatcgtca gcgagagggc cagttccacc agcccgccgc tcgcggccag ttggccaccg
     3421 tcgacgacga ccccgatcac tcccgacatg gcctcgggct tccggaagag gtcgggcagg
     3481 aggttgagga cgtgccagca cgaccccgcc acctgggcga agggggctac ggccagtgcg
     3541 tagaacgatc gcccacccgc catcccgttg taggccgcga aaagggggag cgcggccgcc
     3601 aggttcgcca ggggcccgcc caggaagaag accagccgcc ggcggagggc gatggcgcgg
     3661 aacccctcgt ctccgccgtc cgcgtcgagc gccggctgca catagccgcc cagcggcagg
     3721 gcgcgcaacg agtactccgt cgctccccag cgccggctcc agacccgtgg cccgaagccg
     3781 accgagaagc cggacaccgg aatgcccgcc agcctggcgg ccaccaggtg ccccgcctcg
     3841 tggatcagga tcagcaggct cagcaggatc agcactccca ggtagatcat ggggtcaacc
     3901 tcctccttac atcatggcac tttcgtcatc gcgggaggag ccccccgtcc caggaatccc
     3961 tgccggacca cggctgttcc ctcgtagggt cctgtccatg tcccctgcaa gggacgcccc
     4021 tgaaacttac cagccctgcc ggcgtactag ccgggtgaac gacgaacgct cccggaggaa
     4081 ttcgatgctc agcatttcca atctctcgaa aacctattcc aacggcgtcc aggcgctgcg
     4141 tgacgtgtcg ctggacatcc ccaggggcat gttcggtctc ctcggcccga acggcgccgg
     4201 caagtcgacc ctgatgcgga ccatcgccac cctgcaggac ccggacggcg gcagcatcac
     4261 cctggacggg ctcgacgtgc tccgccagaa ggacgaggtg cggaaggtcc tcggctactt
     4321 gccccaggag ttcggcatct atcccaggat ctcggccgag gtcatgctcg accacttcgc
     4381 gctgctcaag gggatcacca acgaccgcga gcgcaaggag gtcgtgcagg ccctgctcca
     4441 tcagaccaac ctgtgggacg tgcggcggcg caacctgggc acctactcgg gaggcatgcg
     4501 gcagcgcttc ggcatcgccc aggcgctcct cggcaacccc aagctggtca tcgtggacga
     4561 gcccacggcc ggcctcgacc cccaggagcg caaccgtttc ctcaacctgc tctccgagat
     4621 cggcgagaac gtcgtgatca tcctctcgac ccacatcgtc gaggacgtga gcgacctgtg
     4681 cagccgcatg gccatcatct cccaggggcg ggtgctgctc accggcgagc cgcagaaggt
     4741 gatcgatacc ctgcgcggcc ggatctggca gaaggcgatc gacaagagcg agctgccgga
     4801 ctaccaggcg cgctacgagg tgatctccac ccggctgttc gccggccgca cggtcctcca
     4861 cgtccacagc ctcgagaacc cgggcaccgg cttcgagccc gtcgattccg acctcgagga
     4921 cgtctatttc catacgctcc gccaggcgga ccccaaggtc gcggccaagg cggcctgaca
     4981 cctacgcggc gaggtcggat cgatgttcaa agccatctcc actttcgagg tccgctatca
     5041 gttgcggcag cctcttttct atgccgtcac gttgctcctg gcgttgatga ccttcggcgc
     5101 cgtcacctcc gacacggtgc agatcggcgg cagcatcggc aacgtgcacc gcaacgcgcc
     5161 gctcgtcatc atgcgcctcc tcggggccat gaccctgatc ggcatgttcg tcaccaccgc
     5221 gttcgtcgcc agctcggcgc agcgggattt cgagcacggc acccactcgc tgttcttctc
     5281 gaagccgctc aacaagcgcg actatctgct ggggcgcttc ttcggctcgg tggggatctc
     5341 ggccctggtc tttctcggac ccacgctcgg catcctgatc ggcaccttca tgccatggct
     5401 cgagcccgag cggctcggac cgttcaccct cgcgccctac ctctacacgt tcctgatctt
     5461 cgtgatcccc aacctggtct tcaccggcgc ggtgttcttc acgctggcga gcgtcacccg
     5521 cagcctgctc tacacctacc tgggagtcgt cgtcctcttc atcggctaca tcattacgca
     5581 gtcgatgctc cgggacgtcg aaaacgtttt
//
LOCUS       DASPJI010000750         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_447490, whole genome shotgun sequence.
ACCESSION   DASPJI010000750 DASPJI010000000
VERSION     DASPJI010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447490"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2048
                     /locus_tag="VEL74_21060"
     CDS             <1..2048
                     /locus_tag="VEL74_21060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65086.1"
                     /translation="TVEYSTDLFDATTIRRFVGSFQTLLTAAAANPAARLADLPLMNE
                     LERRQVLGEWNDTAVAFPREMPVTRLVEARAAAAPESVAIVDGARVLTYGELNRRANR
                     LARYLRCLDLGADRVVPILLGRSAEMVVAALAVLKAGAAYLPIDPCYPADRVAFMVED
                     AGASVALTRTTLAASLPSQCELALLVDLVGGAGTVWEILAEESGADLGVDIQPESLAY
                     VIYTSGSTGRPKGVAVQHASLTNLVHWHIRSYELTAEDRGCLTAGPAFDASVWELWPC
                     LAAGARLLVPEESVRLDATRIVEWLRREQVSVCFLPTPVTEAILGLETPELPDLRALL
                     TGGDRLHRPACSALPFSLINHYGPTESTVVATCGRVEAGIAHDPTIGRPIDNIRVCLL
                     DTTGQPVPMGVPGELCIGGGGLARGYLGRPELTAEKFVPDPFSGEAGARLYRTGDLAR
                     LLPTGELAFLGRLDHQVKVRGLRIELGEIESALTRHPLVEQAVVLVQTDRKGDQRLVA
                     YVKVAEEPSAAELRKLLRTTLPDYMVPAAFVPLEELPLTPNGKVDRRALPELGAAADP
                     PRGEVEFVAPRTPLEEALAEIWREVLGIERVGVRDRFWDLGGHSLLASKVLARVQDTF
                     GVDLPIQILFEHPVIEDLTGAIGHALLADSGEDAAHVLAELDGLSEEELELLLANPA"
BASE COUNT          330 a          890 c          873 g          373 t
ORIGIN      
        1 gcaccgtcga gtacagcacc gatctcttcg acgccaccac catccggcgc ttcgtgggct
       61 ccttccagac gctcctgacc gcggccgccg ccaaccccgc cgcccgcctc gccgacctgc
      121 cgctgatgaa cgagctggag cgccggcagg tgctggggga gtggaacgac accgcggtgg
      181 ccttcccccg cgagatgccg gtaacgcgcc tggtggaggc gcgggccgcg gcggcgccgg
      241 agtccgtggc gatcgtcgac ggcgcgcggg tcctcaccta tggcgagctg aaccgccgcg
      301 cgaaccgcct ggcccgctac ctccgctgcc tggacctggg cgccgaccgg gtggtcccca
      361 ttctcctcgg gcgctcggcc gagatggtgg tggccgcgct ggcggtgctc aaggccggcg
      421 ccgcctacct gccgatcgat ccttgctatc ccgcggaccg ggtggccttc atggtggaag
      481 acgccggcgc ctccgtggcg ctgacccgga ccactctcgc ggcgtcgctc cccagccagt
      541 gcgagctggc gctgctcgtc gacctcgtgg gcggcgcggg gacggtctgg gagatcctgg
      601 cggaggagag cggcgcggac ctcggtgtcg acatccagcc cgagagcctg gcctacgtca
      661 tctatacgtc gggctccacc ggccggccca agggggtggc ggtgcagcac gcctcgctca
      721 ccaacctggt gcactggcac atccgctcct acgagttgac ggcggaggac cgtggctgcc
      781 tcacggcggg cccggccttc gacgcctcgg tctgggagct ctggccctgc ctggccgccg
      841 gggcgcggct gctggtgccg gaggagtcgg tgcggctgga cgcgacccgc atcgtcgagt
      901 ggctgcggcg cgagcaggtg agcgtctgct tcctgccgac gccggtcacc gaggcgatcc
      961 tcggcctcga gaccccggag ctgccggacc tgcgcgccct gctcaccggc ggcgaccgcc
     1021 tgcaccgccc ggcgtgctcc gcgctcccct tcagcctgat caaccactac ggcccgaccg
     1081 agagcaccgt cgtcgccacc tgcgggcggg tcgaggccgg catcgcgcac gaccccacca
     1141 tcggccggcc aatcgacaac atccgggtct gcctgctgga taccacgggc cagccggtgc
     1201 cgatgggcgt cccgggcgag ctgtgcatcg gcggcggcgg cctggcccgc ggctacctgg
     1261 gccggccgga gctgacggcg gagaagttcg tccccgatcc gttctccggc gaggcgggcg
     1321 cgcggctcta ccgcacgggc gacctggcgc ggctgctccc cacgggcgag ctggccttcc
     1381 tgggccgcct cgaccaccag gtcaaggtgc gcggcctgcg catcgagctg ggggagatcg
     1441 agtcggcgct cacccggcat ccgctggtgg agcaggcggt ggtgctggtg cagaccgacc
     1501 gcaagggcga tcagcggctg gtggcctatg tgaaggtggc ggaggagccg tcggccgccg
     1561 agctgcggaa gctcctgcgc accaccctgc cggactacat ggtgcccgcg gccttcgtgc
     1621 cgctggagga gctcccgctg accccgaacg gcaaggtcga ccgccgggcg ctgccggagc
     1681 tgggcgccgc cgccgacccg ccacgcggcg aggtggagtt cgtggcgccg cgcacgccgc
     1741 tcgaggaggc gctggccgag atctggcgcg aggtgctggg catcgagcgc gtcggcgtcc
     1801 gcgaccgctt ctgggacctc ggcggccact cgctgctggc gagcaaggtg ttggcccgcg
     1861 tccaggacac cttcggcgtc gacctcccca tccagatcct cttcgagcac cccgtcatcg
     1921 aagacctgac cggcgccatc ggccacgccc tcctcgcgga cagcggcgaa gacgccgccc
     1981 acgtcctggc ggagctggac ggcttgtcgg aggaggagct ggagctcctg ctggcgaacc
     2041 cggcctgagc cgcccgaggc ggctcgaagc accttccaca tccacgcgcg gcgcccctcg
     2101 gccagggcgc cgcgtgtgtc ctgtgtccat cgattgtcaa agagcgccaa gaacgcccct
     2161 acgctgccgc agggtcatcc ttccgtgggg cgatggtacc atgaaatcgt ctatgttgat
     2221 ccagttgacg tagatagatt ctggttgtag ctttgccatg cgggacgtct caccgttgag
     2281 gcatcatccc tcagtgttga gggttgttcc cgtactgctg aggacccgcg gcgaagcgtt
     2341 gagggatggc cgcggagcac tgagggacga gccctcaata ctgaggcgtc gaagcggcat
     2401 gctccggagt catccctcag tcttgaggag ccgctccgaa gcactgaggg ctctttcctc
     2461 aacgcc
//
LOCUS       DASPJI010000751        13601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_105208, whole genome shotgun sequence.
ACCESSION   DASPJI010000751 DASPJI010000000
VERSION     DASPJI010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13601)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13601
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105208"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3530)
                     /locus_tag="VEL74_21065"
     CDS             complement(<1..3530)
                     /locus_tag="VEL74_21065"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65087.1"
                     /translation="MTDPSSLSHLSREERALLFERLREKKKRTGVPDGRIPRRAPDLD
                     PVPLSFAQERLWLLDRLMPGTVAYNVVLALSIEGTLVPAALGGALRELMRRHETLRTT
                     YGERQGRGVQIIAPPASVASRRELRMVDLGALPAAAREAEAWRLAAGEAARPFDLQHG
                     PLLRATLLRLGETSHMLLLEVHHIATDGWSMGVMTREIPALYTAASAGRPSPLPEPAL
                     QYADFGVWQRQWLSGEVLERQLAYWRSRLAGLPALQLPTDRPRPAEPSFRGATVRSSF
                     GTGRTRAAVRALAREHEASLFMILLAAFQALLERHTGQRDFAVGTPIANRNRAETEGV
                     VGCFVNSLVLRGNLGGDPRFSDLAGQARKTALEAFAHQDLPFDRLVEALRPERRLAHN
                     PLYQVSIGVQNAPVERVKLPGLVLETLESHAPASRFDLELSFWEDIDDDRLMIQVNYS
                     TDLFDASTVRRMTGHLEVLLAGVLEDPSRRLSSLPLLTGHETHQVLREWNDTAAALPE
                     ATLVDLFREQAARRPGAAAVSSAAEGELSYADLDARSERLARRLAALGVGPDLPVALV
                     ADRTHAMVVGVLAILKAGGAYMPLDPTYPAERLSWMLADSGALILLGEPALLETLPAG
                     VWAPVVVELTADPEETDPDTIPVGPLADALAYVMYTSGSTGQPKGVGVTHRNVVRLVR
                     ESGFADLGPEQAFLQLAPISFDAATLEIWAPLANGGRLALPPSGPLSLDEIGHAIESR
                     GVTSLWLTAGLFHQMVEHRLEALRPLSQLLAGGDVLSPAHVERTLDGLPGLTLINGYG
                     PTEGTTFTTCQPMTAASAPAAGVSVPIGRPIGNTRVLLLDEALRPVPMGVYGELYAAG
                     GGVARGYTGRPDLTAERFVPNPLGQPGERMYRTGDLARFLPAGVVEFLGRRDGQVKLR
                     GYRLELGEVEAVLARHPEVSEAAVALREDVERPGDRRLVAYVVPRIAAPPEDGGDGAV
                     ELDTALSATLRRFLGEELPEYMVPSAFVAMAALPLSPNGKVDRAALPALPDTLAAGHG
                     GDGAKRYVAPRTPVEVRIAGLYAEVLRLERVGLHDDFFEVGGHSLLATQVVSRLRDTL
                     GVELPLYRMFESPTVAGLARLVEDELSLLAAGAAAEVAPPIAPVPRDAGNSDLPLSFA
                     QERLWVIDRLIPGSAAYNIALALSIEGALEP"
     gene            complement(3740..4387)
                     /locus_tag="VEL74_21070"
     CDS             complement(3740..4387)
                     /locus_tag="VEL74_21070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HYG65088.1"
                     /translation="MEKAEAVPDPHAITTVEQLAQLFGEVGEASLKKEVSFIHPHYRA
                     LIEASPFAILATCGPGGLDVSPRGDPPGFVVVEDEKTLLVPERRGNNRIDSLRNLVAD
                     PRVALLFLIPGVGESLRVQGRARITTEPALLARFTMDGKPPKCVLVVQVESVFFQCAR
                     ALLRSRLWEPESWAAERTVPSPGTILAAVSALGESRIDGEKYDRELPERQRSTLY"
     gene            complement(4387..4752)
                     /locus_tag="VEL74_21075"
     CDS             complement(4387..4752)
                     /locus_tag="VEL74_21075"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HYG65089.1"
                     /translation="MEAKEALLDASNLILRAICSHDAAALSEHLAPDFWLLGSGARQD
                     RDGFLEAVRTGDFLTLGASFETIDVEVLGDTAVVAGVQRVEVQVGSERAVSRSVFTDV
                     FVLQGGLWLLRVVRSLELA"
     gene            complement(4776..5345)
                     /locus_tag="VEL74_21080"
     CDS             complement(4776..5345)
                     /locus_tag="VEL74_21080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65090.1"
                     /translation="MIYETFNYEEWTGARLGEPLLIDVFAAMVNIGPADALVASIEAG
                     ATTFCTSAPALTVSGHGGPSGRICLKDLNADGRFEQWRSPDGPPARRNWADLKTPAAY
                     TTGPFMAPTGGGFKYQLLYQGLSSGVVRLLYREYVDNLIRPAFQQDLSYTLAGSGKTE
                     ISFRTTRIEIHSADNNGIRYTVLRGLDAK"
     gene            complement(5517..5741)
                     /locus_tag="VEL74_21085"
     CDS             complement(5517..5741)
                     /locus_tag="VEL74_21085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012323887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HYG65091.1"
                     /translation="MTSRQDRDIEKDYPKADFVAKLRRLADAIESGERFEIQIAGERI
                     YVPVRATFNIEHERSEGEEEIEFQIKWERE"
     gene            complement(5778..6245)
                     /locus_tag="VEL74_21090"
     CDS             complement(5778..6245)
                     /locus_tag="VEL74_21090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65092.1"
                     /translation="MMRIMVVVALFLACLGTASAGEKVVHQLKGEHDYSLICPCEFGR
                     SVGGPDEGSYGALDSSGGMDIIFGIAPNEDAPFARINLGKEDIRLTPVGRSTHECKPG
                     QVWSPRWKAGDVSVSAKLTVDGPGEESCWFTGTLSVKTKEKSETVGIKGACGC"
     gene            complement(6715..7278)
                     /locus_tag="VEL74_21095"
     CDS             complement(6715..7278)
                     /locus_tag="VEL74_21095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65093.1"
                     /translation="MNTFDYLSVLLSIVVGLGLSRMLGNFGELVLQRAKVRFYWVPLV
                     TAAIVFLAHVEFWWSAFGFREIPEWNFFSFILLLFVTIALYMMSVFVLPDPQGEASVD
                     LYAHYYSVRPWFFSTACLYLVLDICFDILVDGEPFLDGVRLFQAAYVGLLTCAAATNR
                     PRLHQSVAVLTLLLFLIQITMLGLEIG"
     gene            complement(7293..7487)
                     /locus_tag="VEL74_21100"
                     /pseudo
     CDS             complement(7293..7487)
                     /locus_tag="VEL74_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003789471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7526..7750)
                     /locus_tag="VEL74_21105"
     CDS             complement(7526..7750)
                     /locus_tag="VEL74_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015124737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65094.1"
                     /translation="MKEDRLVEQRVTYTLEHEGKLYVVENVPARVDEETGEQFFAPST
                     VERLQQVILGEQRPDRVVETPVYKYREKVA"
     gene            complement(7747..8055)
                     /locus_tag="VEL74_21110"
     CDS             complement(7747..8055)
                     /locus_tag="VEL74_21110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002779213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HYG65095.1"
                     /translation="MIEEIRKKVEADEFEFSKHAVDQSIIRRISVQELREAISCAEVI
                     EDYPEDKYGPSCLLYGRTKGGRPIHVQCSYPFQALMKVITVYEPSPERWIDFKVRRSV
                     "
     gene            complement(8226..8720)
                     /locus_tag="VEL74_21115"
     CDS             complement(8226..8720)
                     /locus_tag="VEL74_21115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65096.1"
                     /translation="MSGVPHLPNAFTVGFLARLGERDEPPTAGEAAVAGPWRIEPLSG
                     KGFGLFRAGRSLARGHRPAAVCPSRWVALAIAAMLPGTGRDPMLRLGKDADGEGLYPV
                     TLDTGEVVARLEVFDDTLVEHVNALIHLLQSRCPWRMPWKPRDLKLSKAAGASSTRGC
                     PRPA"
     gene            complement(9516..10016)
                     /locus_tag="VEL74_21120"
     CDS             complement(9516..10016)
                     /locus_tag="VEL74_21120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65097.1"
                     /translation="MRLTRTLLPLVAGLIFTLFVSAPAVADLVQVPGTETSLQPPQGF
                     LLAAKFPGFERADLNASIMVTEMRAPAPEVMKAMTQEGLASRGMKLISSKTETIGGRE
                     ALLLNVAQTAVGTEYLKWMLVTGDPKNTLIVVGSFPKTAEGELSEVVRKAVLTTSWSP
                     GGGVTH"
     gene            complement(10052..10558)
                     /locus_tag="VEL74_21125"
     CDS             complement(10052..10558)
                     /locus_tag="VEL74_21125"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYG65098.1"
                     /translation="MLVLLVGPKGSGKSHISRILEARLGVHFFHAEPLWMAFHAECKA
                     AGRQPTIPEGIARVHPLVARALREHEHVCIETTGASPEILMDLLSLAPEPERLIVRVK
                     VPLDLCLQRVMTRDPTHQIPTELEMVRQVHALSEALELEPDLVLENADLTESEIVALF
                     REALEDLL"
     gene            complement(10572..10754)
                     /locus_tag="VEL74_21130"
     CDS             complement(10572..10754)
                     /locus_tag="VEL74_21130"
                     /inference="COORDINATES: protein motif:HMM:NF038153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I lanthipeptide"
                     /protein_id="HYG65099.1"
                     /translation="MKKTALKKLALNKETLRSLHETQLPEVAGEAPGGTSLQYSRCET
                     CGIIPCSNTCVPSNCG"
     gene            complement(10838..12541)
                     /locus_tag="VEL74_21135"
     CDS             complement(10838..12541)
                     /locus_tag="VEL74_21135"
                     /inference="COORDINATES: protein motif:HMM:NF025627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HYG65100.1"
                     /translation="MRPVLLALIPLLAVCLAFALGCDRGPEAAPVESPRPAASATVSL
                     GKAGPISPRWVAVYDPERAWNGYTLTLHEARVPILLDMNGRPVHAWPEARLKSRVRLL
                     PDGSILGIGLGRLVVEYDWEGRQVWEFRTPGAIPHHDVLRLANGNTLVLVLKEGDVAD
                     TLYEVDRAGKVVWTWQAAEHLKDRFPEQPSDPDDLTHINSLQELPENPWFAAGDARFR
                     PGNLLVSARNLNAAFIIDRSNGEVVWWFSEGLDAQHEALMNGPGLPEAGKIQIFNNRL
                     HSFSDDRQSEVLEIDPRNGSVDWRYRSAGFFSPTSATQQALPNGNVLITSTRGGRVFE
                     VTRAGETVWEWVPPYQTVRALRIAADACPQLARLTVPAPEAVAPAAGYRHVDRDVYRF
                     SRQGARTKAVVDGQERTVLKRETDCRDLLLPASAKLQVGYGVDRERLRAAGRESRPPE
                     FAVRLRPEGAGEDVELLRETVGLDGTSWRQRTLPLESYGLQTVQLCVEIDGGAAGASQ
                     RGRFAFWEQPLILTPRDLARAGEAEDGDDDGAADQAPADLTPEELEVRRKHLKSLGYV
                     G"
     gene            complement(12582..12911)
                     /locus_tag="VEL74_21140"
     CDS             complement(12582..12911)
                     /locus_tag="VEL74_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG65101.1"
                     /translation="MNDNSPLGTFEEQVMLAVLRTGEEAYGMNVRRELERVTGREVTI
                     GAVYATLDRLEAKGLVSSSRSGAGGASRRLFAVTAPGARGLAESRMMRERLWEGVDLR
                     PLLAPGG"
     gene            complement(12931..13584)
                     /locus_tag="VEL74_21145"
     CDS             complement(12931..13584)
                     /locus_tag="VEL74_21145"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYG65102.1"
                     /translation="MGARIIGVVENVAEARLTDPQQPARYVLTEQVPYTPELQTLVLR
                     TERPEDAAAILSVARSAVQKAAPGVAVQEATTMEQVFAQAVGPVRQIMTLLTLLTGLA
                     LVLGAIGVYGVIAHHVSRRKRDYGIRVALGLPPSRVMQQVVGRGAALVGLGAVIGVVA
                     ALLLARLLASLLYGVGAADPMALTAATVALLVVGVLAAFVPAYRASRVDPARVLYEP"
BASE COUNT         2299 a         4766 c         4456 g         2080 t
ORIGIN      
        1 ggctccagcg ccccctcgat ggagagcgcc agggcgatgt tgtacgccgc cgagccgggg
       61 atcagccggt cgatgaccca gaggcgctcc tgggcgaacg agaggggcag atcgctgttg
      121 ccggcatccc gcggcaccgg cgcgatcggc ggcgccactt ccgccgcggc gccggccgcc
      181 agcagggaca gctcgtcctc caccagccgc gccagcccgg ccaccgtggg cgactcgaac
      241 atccggtaca gcggcagctc cacccccagg gtatcgcgca gccgtgacac cacctgggtg
      301 gcgaggaggg agtgccctcc cacctcgaag aagtcgtcgt ggaggccgac ccgctccagc
      361 cgcagaacct cggcatagag gccggcgata cgcacctcca ccggcgtgcg cggcgccaca
      421 taccgcttcg cgccgtcgcc gccgtgcccg gccgccagtg tgtccggcag ggccggcagg
      481 gccgcccggt ccaccttgcc gttgggcgaa agcggcagcg ccgccatcgc cacgaacgcc
      541 gaggggacca tgtactccgg cagctcctcg cccaggaagc gacgcagcgt ggcgctcagg
      601 gcggtgtcga gctccacggc cccgtctcct ccatcctctg gcggagcggc gatccggggg
      661 accacatacg ccacgagccg ccggtccccc ggcctctcca cgtcctcgcg caacgccacc
      721 gccgcctcgc tcacctccgg atgccgtgcc agcacggcct cgacctcccc cagctccaac
      781 cggtagccgc ggagcttcac ctgcccgtcg cggcggccga ggaactcgac cacgccggcc
      841 ggcaggaagc gcgccaggtc ccccgtccgg tacattcgct cgcccggctg cccgagggga
      901 ttgggcacga accgctccgc cgtcaggtcc ggccgccccg tgtagccccg cgccacccct
      961 cccccggccg cgtacagctc accgtagacc cccatgggca ccggccgcag cgcctcgtcc
     1021 agcagcagca cccgggtgtt gccgatcggc cggccgatgg ggaccgacac ccccgctgcc
     1081 ggcgccgacg ccgcggtcat gggctggcag gtggtgaacg tcgtcccctc ggtggggccg
     1141 tagccgttga tcagcgtcag cccgggcagg ccgtccagag tccgctcgac gtgggcgggt
     1201 gacagcacgt cgccgccggc caggagctgg gagaggggcc gtagcgcctc cagccggtgc
     1261 tccaccatct gatggaagag ccccgccgtc agccacaggg aggtgacccc ccggctctcg
     1321 atggcgtggc cgatctcgtc gagcgacagc gggccggacg gtggcagggc caggcggccg
     1381 ccgttggcga gcggcgccca gatctccagc gtcgcggcgt cgaaggagat cggggcgagt
     1441 tgcaggaagg cctgctccgg ccccagatcg gcgaagccgc tctcccgcac cagccgcacg
     1501 acattgcggt gggtgacgcc tacccccttc ggctggcccg tcgatcccga ggtgtacatc
     1561 acataggcga gggcgtcagc cagcggcccc accgggatgg tatccggatc ggtctcctcc
     1621 gggtcggcgg tcagctccac caccaccggc gcccagaccc cggcgggcag agtctccagc
     1681 agcgccggct cccccagcag gatgagagcc ccggagtcgg cgagcatcca cgacagccgc
     1741 tccgccggat aggtgggatc gagcggcatg taggcgccgc cggccttgag gatcgcgagc
     1801 acgcccacca ccatggcgtg ggtacggtcg gcgaccaggg ccaccggcag gtcggggccc
     1861 acccccaggg cggccagacg ccgcgccagc cgctccgagc gcgcgtccag gtcggcataa
     1921 gaaagctccc cctcggcggc ggatgagacc gccgccgccc cgggccgccg cgcggcctgc
     1981 tcacggaaga ggtccactag cgtcgcttcg gggagtgcag ccgcggtgtc gttccactcg
     2041 cggagcacct gatgggtctc gtgcccggtg agcaggggca ggctggatag ccgccgcgac
     2101 gggtcctcca gcactccggc cagcagcacc tccaggtgtc cggtcatccg ccgcacggtg
     2161 gaggcgtcga agaggtcggt gctgtaattc acctgaatca tcagccggtc gtcatcgatg
     2221 tcctcccaga acgacagctc gaggtcgaag cggctggccg gggcgtggct ctccagggtc
     2281 tccagcacca ggccgggcag cttgacgcgc tccaccggcg cgttctgcac tccgatcgag
     2341 acctgataga gcgggttgtg cgccagccgc cgctcggggc gcagcgcctc caccagccgg
     2401 tcgaagggga ggtcctggtg ggcgaaggcc tccagcgcgg tcttgcgcgc ctgccccgcc
     2461 agatccgaga accgtggatc accgccgagg ttgccgcgca gcaccaggct gttgacgaag
     2521 cagccgacca cgccctccgt ctcggcgcgg ttccggttgg cgattggggt gccgaccgcg
     2581 aagtcgcgct ggccggtgtg gcgctccagc aaagcctgga aggcggccag gaggatcatg
     2641 aacaggctgg cctcgtgctc gcgcgccaga gcccgtacgg ccgcccgcgt tctccccgtc
     2701 ccgaaggagg accggaccgt ggcgccgcgg aagctcggct ccgccggccg cggccggtcg
     2761 gtggggagct gaagcgccgg cagcccggcg agccggctcc gccagtaggc gagctgccgc
     2821 tccagcacct cgcccgagag ccactgccgc tgccagacgc cgaagtcggc gtattggagg
     2881 gccggctcgg gcaggggcga aggccggccg gcgctcgccg cggtgtagag ggcggggatc
     2941 tcccgcgtca tcacccccat cgaccagccg tcggtcgcga tgtgatggac ctcgagcagc
     3001 agcatgtggc tggtctcgcc cagccgcagc agggtggcgc gcaacagcgg gccgtgctgg
     3061 aggtcgaacg gccgcgcggc ctcgccggcg gcgagccgcc atgcctcggc ctcgcgcgcc
     3121 gcagccggca gggcgccgag gtcgaccatc cgcagctccc gccgggaggc tacggaagcg
     3181 ggcggggcga tgatctgcac cccccggccc tgcctctccc cataggtcgt gcgcagcgtc
     3241 tcgtggcgcc gcatcagctc gcgcaacgcg cctcccagag cggccgggac cagcgtcccc
     3301 tcgatcgaca gggcgaggac gacgttgtac gccaccgtcc cgggcatcag ccggtcgagg
     3361 agccagaggc gctcctgggc gaacgacagc ggcacggggt cgaggtccgg cgcacggcgg
     3421 gggatccgcc cgtcgggaac gccagtccgc ttcttcttct cgcgcaagcg ctcgaagaga
     3481 agggccctct cctcccgcga gagatggctg agcgagctgg ggtccgtcac gcggcctcct
     3541 cttccggagt ggcgcttcgt cgttgaatgg aaagggaaaa gacctggaac ggaccgacca
     3601 tggacatgcc tcgggtacaa ccttatcagc gccggccgcg ggagcacacc tccaatcccc
     3661 atccggcccc ggatgtgcgg cttctacctc caaggtgtcc gatggatcac gagcgagaca
     3721 accggagaga atttaatgat cagtaaagtg ttgaccgctg ccgctcggga agctcccggt
     3781 cgtacttctc gccgtcgatc cgggactcgc cgagcgcgct gaccgcggcg aggatcgtgc
     3841 ccgggctcgg cacggtccgc tccgccgccc acgactccgg ctcccacagc cgggaccgga
     3901 ggagcgcccg ggcgcactgg aagaagacgc tctccacctg gaccaccagg acgcacttgg
     3961 ggggcttgcc gtccatcgtg aagcgcgcga gcagggccgg ctccgtggtg atgcgcgccc
     4021 ggccctggac gcggagcgac tcgccgacgc cgggaatcag gaacaggagc gccacccggg
     4081 gatcggccac gaggttgcgc aggctgtcga tccggttgtt cccccgccgc tccggaacca
     4141 gcagcgtctt ctcgtcttcg accacgacga atcccggtgg atcgccgcgc ggcgagacgt
     4201 ccaggcctcc gggcccgcac gtcgcgagga tcgcgaacgg cgacgcctcg atgagcgccc
     4261 ggtagtgggg atggatgaac gaaacctcct tcttgaggga ggcttcgccg acctcgccga
     4321 agagctgcgc aagctgctcg acggtggtga tggcgtgggg gtccggcacc gcctcggcct
     4381 tctccatcac gccagctcca gcgaacgtac gacgcggagg agccagagcc ctccttgaag
     4441 aacgaacacg tccgtgaaca ccgaccgtga aaccgcgcgc tcgctgccga cctgtacctc
     4501 cacccgttgc acccccgcga cgaccgcagt atcccccagg acttcgacgt cgatcgtctc
     4561 gaaagaggcc ccgagagtaa ggaaatctcc ggtccgcacg gcttccagga acccgtctcg
     4621 gtcttgccgt gcacccgagc ccagcagcca gaaatcggga gccagatgct cggacagcgc
     4681 cgccgcgtca tgagagcaga tggcgcggag aatgagattg ctcgcgtcca ggagcgcttc
     4741 cttggcttcc atggttcccc ctccgagcgg cgctctcact tcgcgtcgag tccacggagg
     4801 accgtgtagc ggattccgtt gttgtccgcc gaatggatct cgatacgggt ggttcgaaac
     4861 gagatctctg tcttgccgct gccggcgagg gtataggaga gatcctgctg aaacgccggc
     4921 cggatcaggt tgtcgacgta ttcccggtag agcagcctga cgacgcccga ggaaagcccc
     4981 tgatacagaa gctggtactt gaaccctcct ccagtcggag ccatgaaggg gccggtggta
     5041 tacgcggcag gcgtcttgag atcggcccaa ttccggcgcg ccggcgggcc gtccggcgag
     5101 cgccactgtt cgaaccgccc gtcggcattc aagtccttca ggcagatccg gcccgacggt
     5161 cccccgtgtc cgctcaccgt cagagccggc gcggacgtgc agaaggtggt cgcaccagcc
     5221 tcgatgctgg cgaccagagc gtcggccggc ccgatattca ccatggccgc gaagacatcg
     5281 atcagcaacg gctctcccag ccgggcgccg gtccactctt catagttgaa cgtctcgtag
     5341 atcacgtctc ccacggatcg ctccgtagcc tcgccgatcg ccggtgcctc tcccaccttt
     5401 cgcgaaacct gaggcgccgg cagcggcacg gaagcacacc ccacgagcga gagaagccat
     5461 gcggcgatga agatcctctt cgtcacgctc accatagacg tgcctgggtc cagcccctac
     5521 tcccgctccc acttgatctg gaactcgatc tcctcctcgc cctcggaccg ctcgtgctcg
     5581 atgttgaacg tcgcccgtac gggcacatag atccgctcgc ccgcgatctg gatctcgaac
     5641 cgctctccgc tctcgatggc atctgcgaga cgccgcagct ttgccacgaa atcagccttc
     5701 ggatagtcct tctcgatgtc ccgatcctgt ctcgatgtca tatcgccttt ctccgcttac
     5761 tggtagaagg tgtgccttca acagccacac gcacccttga tgcctaccgt ctccgacttc
     5821 tccttggtct tgacgctcag ggttccggta aaccagcagg attcctcacc tggcccgtcg
     5881 accgtgagct tcgccgaaac cgacacgtcg cccgccttcc aacgaggaga ccacacctgt
     5941 cccggcttgc actcgtgcgt tgagcggcca accggagtca gccgaatatc ttcctttccc
     6001 agattgattc gcgcgaaagg cgcatcctca ttgggagcga tcccgaagat gatgtccatc
     6061 cctccggaac tgtcgagagc gccgtaactg ccctcatcag gcccgcccac ggagcgcccg
     6121 aactcgcagg gacagatcag cgagtaatca tgctctccct tcaattgatg gacaaccttc
     6181 tcgcccgcgc tcgcggttcc caggcaggcc aggaacaagg ctacgacaac catgatccgc
     6241 atcacctgat ctcctttccg aagacctcat gtctccggta agttttcgat ttaccatgcg
     6301 tggcatcggc tctcacccgc cactcggcgc cggagaaccc ggagtcgagc agggcgtgga
     6361 gcgccacgtt gtcccgagga ggcgtggtgg acgcgacgtc cgcgggcccg aacgggtagg
     6421 ccagagtatg cccccggagc cgcccgaaga acaagcggct cccgggcagg aggatcggac
     6481 cttcccaacc ctcttccccg ctgcgggaag gctgacgatc tgacagaatt tgagcggcaa
     6541 atacagcctc atcgttcgaa gtgatcggat cgaagcaccg gcggaaaggg acacctgaca
     6601 ctccgcgtgg gaatagggcg tcgcaaagac ctgccgctcg aagaagcggt gccgagcagc
     6661 gaaacctcac ggacatgagt cggctccgcg acgtatcgaa tcggcttgtc cacctcaccc
     6721 gatctccaac cccagcatgg tgatctgaat caggaagagc aacagtgtga gcacggcgac
     6781 cgactgatga aggcgcggac ggttcgtcgc cgcggcgcac gtgaggagcc cgacgtatgc
     6841 cgcctggaag agccgcaccc cgtcgagaaa cggctcgcca tcgacgagaa tgtcgaagca
     6901 gatatccagc accagataga ggcaggccgt tgagaagaac caggggcgaa ccgaatagta
     6961 gtgcgcgtat agatcgacgc tcgcttcgcc ttgaggatcg ggaagcacga agacgctcat
     7021 catgtagagc gcgatggtca cgaagagcag caggatgaag gagaagaagt tccactcggg
     7081 tatttcacga aagccgaagg ccgaccacca gaactcgacg tgcgccagga agacgatcgc
     7141 cgccgtcacc agcggcaccc aatagaagcg caccttcgcc cgttgcagga cgagctcccc
     7201 gaaattcccg agcatccgcg agaggcccag ccccaccacg atcgagagca ggacggacag
     7261 gtaatcgaac gtgttcatcc cgctcagaac cgcttgcccg gatactcctt ggtcatgcgg
     7321 atgaagtact cgatgaaagc ttccttgccg tcagccacca tggggttgtg ctggacgtag
     7381 acctccccaa cgtagcgctc gaccgcctcg gccggctggc actgattgaa catgaggtcg
     7441 taggaggcgg cgacggtccg cttgttccgc tcgagtcggt cctccatctg gatctcctgg
     7501 cttggcagcc actgtagccg cgctcttatg ccaccttctc ccggtacttg taaaccggtg
     7561 tctcaacgac acgatccggc ctctgctcac cgaggatcac ctgttgcaga cgctcgactg
     7621 tagagggagc gaaaaattgc tctcctgtct cttcatcgac acgcgcagga acgttctcca
     7681 cgacgtaaag tttcccttca tgctccaggg tataagtcac tctttgttcc accaggcgat
     7741 cttccttcat actgacctcc ttaccttgaa gtcgatccac cgttcagggc taggctcata
     7801 gactgtgatc accttcatca gtgcctgaaa aggatagctg cactggacat gtatcggccg
     7861 cccgcctttg gttctgccgt agagcaagca actcggtccg tacttatctt cgggatagtc
     7921 ttcaatgacc tcagcgcagg agatggcctc tcgcagctcc tgcacactga tacgtcgaat
     7981 gatgctttgg tcgacagcat gcttcgagaa ctcaaactca tcggcttcga ccttcttccg
     8041 tatctcttca atcagggaca tgatgattaa tgatcgcgca ctgtattttt ctttatccaa
     8101 agcacataga gagtctagcc cggtggactg aggggagtca aacggacgaa gacaaagcgc
     8161 cgtggcatgc tcccggcgca gcggagcacg ccacggcctc gctacctcga cttaccgggc
     8221 cggcgtcatg ccggacgggg acatcctctc gtcgaggatg ctcccgcagc cttcgagagc
     8281 ttcagatccc gcggcttcca gggcatccgc cagggacaac gggactgcaa gagatggatg
     8341 agggcgttga cgtgctcgac cagagtgtcg tcgaagactt cgagccgcgc caccacctcg
     8401 cccgtgtcca gtgtgaccgg gtacaggccc tccccgtccg cgtccttgcc gagccggagc
     8461 atcggatcgc ggccggtgcc ggggagcatt gcggcgatcg ccagggccac ccagcgcgac
     8521 ggacagaccg ccgccggccg atgacctcgg gcgaggctcc ggccggcccg gaagaggccg
     8581 aagcccttcc cggagagcgg ctcgatccgc cacggccccg ccaccgcggc ctcgccggcc
     8641 gtcggcggct cgtcccgctc gccgagacga gcgaggaacc caacggtaaa tgcgttgggg
     8701 agatggggga cgccggacac actggtacta tcgtgggaac cacacatcag gacctccagt
     8761 cctcgtgttc gtggccaggc ccggtcgcga gttgctgctc acttccgggc cgactcggca
     8821 ccctgccggg ccacgtcctg ccttgtacga cgggttcgac tcccacaccg gagagctttc
     8881 gcggggccgc gcgccctccg gcgtgtcctg ctacgacttg atggactgat cctagaagac
     8941 ctcaaagcac aagtcaagcg tctactcgcg ccgggatccc ttgccgatca ggggaggacc
     9001 gatccctcag tgctgaggca cggttcctca acactacacc gccactcctc aacgcttcgc
     9061 cgccctccct caacgcttcg gggcgggccc tcagtgttga ggaacgaggc tgaagcactg
     9121 aggaggctcc cctcaacgtt gaggcgcgac ggcgaagcat tgagggagga cggcggagcg
     9181 ctgaggggac atccctcagt cttgaggaac gacggcgaag cactgaggga cggctcctca
     9241 acgttgagga accgtccctc agcgctccgg agcgcctccg taccactgag gggcttcccc
     9301 tcaacgttga gggatcatcc ctcaacactg aggaacggct cctcagtgct ccggagctct
     9361 ccctcaacgg tgagggatgc gccctcagta ttgagggacc gcggcgaagt gttgaggaac
     9421 gggcccgtac tctcggggag ccggacgtca gccccgaggg gagccccgct gcggtcttgc
     9481 ggcctctctc cctggggcca cgaggccctg gagatttaat gggtcacacc gccgcccggg
     9541 ctccacgagg tggtgagcac ggccttccgg accacctcgc tgagctcccc ctcggcggtc
     9601 ttgggaaaag agccgacgac gatcagggtg ttcttgggat cccccgtcac cagcatccac
     9661 ttcaagtact ccgttccgac cgccgtctgg gcgacgttca gcagcagcgc ttcacggccg
     9721 ccgatcgtct cggtcttcga ggagatcagc ttcatccccc ggctggcgag cccctcctgg
     9781 gtcatcgcct tcatcacctc cggcgccggg gcgcgcatct cggtgaccat gatcgacgcg
     9841 ttcagatcgg cgcgctcgaa gcccgggaac ttggccgcga gcagaaagcc ctgcggcggc
     9901 tggagactgg tctcggtgcc ggggacctgc acgaggtccg cgaccgccgg agcggatacg
     9961 aaaagggtga agatcaaccc cgcgacgagc gggaggaggg tgcgcgtgag tctcatgggc
    10021 gaagagtcat cctttccttc atgtccgccg ctcatagtag gtcctcgagg gcctccctga
    10081 acagggcgac gatctcggat tcggtgaggt cggcgttctc gagcaccaga tccggctcca
    10141 gctccagggc ctcgctgagc gcgtggacct gacgcaccat ctccagctcc gtcgggatct
    10201 ggtgagtcgg gtcgcgtgtc atcacccttt ggaggcacag atccagaggc accttcacac
    10261 gaacgatcaa tctctcgggc tccggcgcca gggagagcag atccatcagg atctccggag
    10321 acgcgccggt ggtctcgatg cagacgtgct cgtgctcacg aagggctcgg gcgacgaggg
    10381 gatggacccg ggcgatgccc tcggggatgg tgggctgccg gccggcggcc ttgcactcgg
    10441 cgtggaaggc catccagagc ggctcggcgt ggaagaaatg tacgccgagc cgcgcttcga
    10501 gaatacggct gatgtggctc ttgccgctgc ccttcgggcc gacgagcagg acgagcacga
    10561 tcagccggga gctagccgca gttgctcggc acgcaggtgt tggagcaggg aatgatgccg
    10621 caggtctcac acctggagta ctggagcgag gtcccgcccg gagcttcgcc cgcaacctcg
    10681 ggcagttggg tctcgtgcag gctccgcaac gtctctttgt taagagcaag ctttttgagc
    10741 gcggtcttct tcatctcgat ctccttcggg cccggtgaga ggtggcccta caactcaagg
    10801 taccaccgac aggaaaagcg ggcccggggc cttcgcctca gccgacgtag ccgagcgact
    10861 tgaggtgctt gcggcggacc tccagctctt cgggggtcag gtcggccggc gcttggtccg
    10921 ccgccccgtc gtcgtcgcca tcctccgcct cgccggcgcg cgcgaggtcg cgcggggtga
    10981 ggatcagcgg ctgctcccaa aaagcgaagc gcccgcgctg gctggcgccg gcggcgccgc
    11041 cgtcgatctc gacgcagagc tggacggtct gcaggccgta gctctccagc ggcagggtcc
    11101 tctggcgcca ggaggtgcca tcgagcccca ccgtctcgcg cagcagctcg acgtcctccc
    11161 ccgccccctc gggacgcagc cgcaccgcga actccggggg ccggctctcc cggccggcgg
    11221 cgcgcaggcg ctcgcggtcg acgccgtagc ccacctggag cttggcggat gccggcagca
    11281 gcaggtcgcg gcaatcggtc tccctcttga gcaccgtccg ctcctggccg tcgacgacgg
    11341 ccttcgtgcg ggcgccctga cgggaaaagc gatagacgtc ccggtcgacg tgccggtagc
    11401 cggcggccgg cgcgacggcc tcgggagccg gaaccgtgag gcgggcgagt tgggggcagg
    11461 cgtcggccgc gatccggagg gcgcgcacgg tctggtatgg cggcacccat tcccagacgg
    11521 tctcccccgc ccgcgtcacc tcgaacaccc ggccgccccg ggtcgaggtg atcaggacgt
    11581 tgccgttggg cagcgcctgc tgcgtcgcgg atgtgggtga gaagaagccc gccgagcggt
    11641 agcgccaatc caccgagcca ttccggggat cgatctcgag cacctcgctc tggcggtcgt
    11701 ccgaaaagct gtgcagccgg ttgttgaaga tctggatctt gcccgcctcg ggcaggccgg
    11761 gcccgttcat cagcgcctcg tgctgcgcgt cgaggccttc cgagaaccac cagaccacct
    11821 ccccgttcga gcggtcgatg atgaaggcgg cgttcaggtt gcgcgccgag accaggaggt
    11881 tgcccggccg gaaacgcgcg tcgccggcgg cgaaccaggg attctcgggg agctcctgga
    11941 gcgagttgat gtgggtgagg tcgtccgggt ccgacggctg ttccggaaac cgatctttca
    12001 ggtgctcggc tgcctgccag gtccagacca ccttgccggc gcggtcgacc tcgtagaggg
    12061 tgtcggccac gtccccttcc ttcaggacga gcaccagcgt gttgccgttg gcgaggcgca
    12121 ggacgtcatg atgcgggatc gcgccggggg tccggaactc ccagacctgc cggccctccc
    12181 agtcgtactc gacgaccagc cggccgagcc cgatgccgag gatggagccg tccgggagca
    12241 ggcgcacccg cgacttgagc cgtgcctcgg gccaggcgtg caccggccgc ccgttcatgt
    12301 cgagcaggat cgggacgcgg gcctcgtgga gcgtcagggt gtagccgttc caggcgcgct
    12361 cgggatcgta gacggccacc caccgcgggg agatcgggcc cgccttgccg agggataccg
    12421 tcgcggaagc cgccggccgg ggcgactcca ccggggccgc ctcgggccct cggtcgcagc
    12481 ccagagcgaa ggcgagacag acggcaagga gaggaatcaa cgcgagcagg acgggacgca
    12541 tgggccccat agagtagcgt atggggcccg ggcgctcatc ctcacccccc cggcgccagc
    12601 agcgggcgca ggtccacccc ttcccacagc cgctcgcgca tcatccgcga ttcggccagc
    12661 ccgcgggcgc cgggcgcggt cacggcgaag aggcgccggg aggcgcctcc ggcccccgag
    12721 cggctggagg agacgagccc cttcgcctcc agccggtcga gcgtcgcgta gacggcgccg
    12781 atggtgacct cgcggccggt gacccgctcc agctcgcgcc gcacgttcat gccatacgcc
    12841 tcctcgccgg tacggagcac cgcgagcatc acctgctcct cgaacgtgcc gagcgggcta
    12901 ttgtcgttca tgaccactct cccggagccc tcacggctcg taaaggaccc tcgccggatc
    12961 cacgcggctc gcgcggtagg cggggacgaa ggcggcgagc acgccgacca ccagcagcgc
    13021 cacggtcgcg gcggtgagcg ccatgggatc ggccgcaccg acgccgtaca gcagcgaggc
    13081 cagcagccgc gcgagaagca gcgcggcgac gacgccgatc accgcgccga ggcccaccag
    13141 cgcggcgccg cggcccacca cctgctgcat cacgcgcgac ggcggcagcc ccagcgccac
    13201 gcggatgccg tagtcgcgct tgcggcggct cacgtggtgc gcgatcacgc cgtagactcc
    13261 gatcgcgccg agcaccaggg cgagcccggt cagcagcgtg agcagcgtca tgatctgccg
    13321 caccggcccg accgcctggg caaagacctg ctccattgta gtcgcctcct gcacggcgac
    13381 gccgggcgcg gccttttgca ccgcggagcg ggccaccgag aggatcgccg ccgcgtcctc
    13441 cggacgctcc gtgcgcagca ccagggtctg gagctccggc gtgtagggca cctgctcggt
    13501 gagcacatac cgcgccggct gctgcggatc ggtgaggcgc gcctcggcca cgttctccac
    13561 cacgccgata atgcgcgctc ccacgccgaa ctcctccagg c
//
LOCUS       DASPJI010000752         4795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_447708, whole genome shotgun sequence.
ACCESSION   DASPJI010000752 DASPJI010000000
VERSION     DASPJI010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447708"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VEL74_21150"
     CDS             <1..235
                     /locus_tag="VEL74_21150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65103.1"
                     /translation="DKTPDADGAYPLILPGPDGGEVVGRFQLFDEAAVDAMNVAVHLL
                     RAPESLAYLLEAAGAVALERCGAILDERTQSTE"
     gene            complement(572..997)
                     /locus_tag="VEL74_21155"
     CDS             complement(572..997)
                     /locus_tag="VEL74_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HYG65104.1"
                     /translation="MRFMVLVKANEDSEAGVMPSEKMLTEMGKYNEELVKAGVMVAGE
                     GLHPSPKGVRIRFDGKDRTVIDGPFPETKELLAGFWILQAKSREEVIEWVKRAPMDGG
                     TELEIRQIFELEDFGAEMTPELREQEERLRQQVEDQQKP"
     gene            1166..2566
                     /locus_tag="VEL74_21160"
     CDS             1166..2566
                     /locus_tag="VEL74_21160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65105.1"
                     /translation="MPTKPILVVTLALALSVPAQAQHTHQHPPPQEPAPPAAEPPPAH
                     EHGAQTTGHERMDHGAEGHEMPGMLGPYGISREASGTAWQPESAPHMGLHTTRGDWML
                     MTHGFLFLVYDDQGGPRGDEDVFSANMLMGMASREAGPGRLGLRAMISAEPWTIGRRG
                     YPLLLQTGETADGVHPLVDRQHPHDLFMELAASYNVPVGEDGSVFVYAGWPGEPALGP
                     PAFMHRFSGMDLPEAPLGHHWLDSTHVTFGVLTLGWIQGDVKLEGSVFTGREPDEDRL
                     DIEEPKMDSWSVRASWQPTPDWSLQASYGFLKSPEQLEPEVDLDRVTASAIYNRPLPD
                     GNWQTTFAWGRNAKDPGETTDTYLLESTLAFAPRHTVFGRAERQENDELFGHGDHGHE
                     GEASDVVDSLEGRVFNVGKLTLGYIHDLFAGDGYQAGIGASWSLALVPEELEEVYGDQ
                     PSSWMAFVRVVLGSEM"
     gene            complement(2576..>4795)
                     /locus_tag="VEL74_21165"
     CDS             complement(2576..>4795)
                     /locus_tag="VEL74_21165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65106.1"
                     /translation="HGQSPETHFLEREPTERLFSQVGELLESAGDDEVLPALFVLGAP
                     GAGKTTLVRRVAARAVQEGLAVVAAPKLNLERIDDGDVSTLLRSLARLEEGTLPVLLV
                     LDDPLFAESGWDDLLRRLAPRSRRLAVLATSPDYLFHEHRHQLGRQVDVRIFPLDRPR
                     RPEREALALLHGRDPGLFQDREDDFLVLAMEASAGASFKSLVDRLWMTLNHGRPIDPR
                     TRPDELAWLVRAYLVVCYWHRFGMTCPEVVLGAILDHSHRDPLAPSCRYELQRLVSEQ
                     GWEVFTVTEPARHLAFLGAGIGASHPRIAAEAWHRRPVPAFDVGEWVARASLAAQPAG
                     VDLGILACRVDEPGLRDRLVELWNRAALDGTLETRNLCLLHAALSLHGAAAAARRLEV
                     GLAACAARLDGQSWLAVLALLHYGKDGAVPPGVDLLSVIRVADLHLAPGRATMLMSAL
                     KDSPDALGAVLDRIGATADYAGTGHLLARLVAARPGDAAVLGRAAAWLRSHEGATGTA
                     ELLVQLVEASPSDPDLVRDALDWLRAHANHPNVRRLLVPLVDANPTHPELRRWALAWI
                     GENEGWPKAQRVLARLVERSGGDEEAMDLALDWLARPQRKKQVRNLMSALGRALAATP
                     AATLRFLSGCNDENRKKLLLEALVRAVQENPEWTGPLLEALEPLPSRDLSLLLLHLLR
                     KRSEPVESFLAAWLADPRFLEDRGSLLRSIRSKPPLRERLLAREDLAPEVRRQLQGT"
BASE COUNT          707 a         1696 c         1678 g          714 t
ORIGIN      
        1 cgacaagacg cccgatgcgg acggcgccta tccgctgatc cttcccggcc cggacggcgg
       61 cgaggtcgtc ggacgcttcc aactcttcga tgaggctgcc gtggacgcca tgaacgtggc
      121 ggtccacctc ctgcgggcgc cggagtccct ggcctacctg ctggaggcgg cgggggccgt
      181 cgccctcgaa cgttgcgggg ccattctcga cgagcggacg caatccaccg agtagtgcct
      241 gctgttcggc ggcggcccgt cagctccgga gggccgccgt ctctctcgga gcctctcccc
      301 gtcccatggc gaaccttcgc cgtccgatgg ccgagctctc ccgacaatgg gcgaagcttg
      361 gccggtgctc ggcttggcgc ggcctgtggt cggcctggcg cggcctgtcg tcggcctggc
      421 gcggcctgtg gtcggcaagg cggggccgat cgacggcgaa gcgcggcctg tggtcggccg
      481 cgcggtgcct gtggtcggcc gagccgtgcc gaaggtcggc ccggcagtgc cggtgttcgg
      541 ccaaggtctg ccaatgaagg gcaaagcctt actacggctt ctgctgatcc tccacctgct
      601 ggcgcaggcg ctcctcctgc tcccgcagct ccggggtcat ctcggcgccg aagtcctcca
      661 gctcgaagat ctgccggatc tccagctcgg tgccgccgtc catgggggcg cgcttgaccc
      721 actcgatgac ttcctcccgg gacttcgcct ggaggatcca gaagccggcg agcagctcct
      781 tggtctcggg gaagggtccg tcgatcaccg tccggtcctt gccgtcgaag cggatacgga
      841 cccccttcgg gctgggatgg agcccctcgc ccgcgaccat cacgccggcc ttcaccaact
      901 cctcattgta tttccccatc tcggtcagca tcttttcgct cggcatcaca cccgcttcgg
      961 aatcctcgtt ggccttgacc agtaccatga agcgcattgt cgtatctcct tgaattggtt
     1021 tcagttgagg agccgggccg ccggtcttgc cggcctcatc cctggctcta ctcattggtc
     1081 gaacgaggga aggcgagatc gacatcggca ggccgattgc aactctgccg ggtaacccgg
     1141 tctccgggtg gggaggagtg tctacatgcc taccaagccg attctcgtcg ttaccttggc
     1201 gctcgcgctc tcggtcccgg cccaggccca gcatacccac cagcacccgc cgccgcagga
     1261 gccggccccg cctgccgccg agccgccgcc ggcccatgag cacggcgcgc agacgaccgg
     1321 tcacgagagg atggaccacg gagcggaggg gcacgagatg ccgggcatgc tgggtcctta
     1381 tggcatctcg cgggaagcct caggcacggc ctggcagccc gagtcggcgc cgcacatggg
     1441 gttgcacacg actcgcggcg actggatgct gatgacccac ggcttcctct tcctcgtcta
     1501 tgacgaccag ggcgggccgc ggggggacga ggacgtcttc tccgccaaca tgctgatggg
     1561 gatggcgagc cgcgaggccg ggccggggcg tctcggcttg cgggcgatga tctccgccga
     1621 gccttggacg atcggccgcc gcggctatcc gctcctgctc cagaccggcg agaccgccga
     1681 tggggtccat ccgctggtgg accggcaaca tccccacgac cttttcatgg agctggccgc
     1741 cagctacaac gtcccggtgg gggaggacgg ctcggtcttc gtctatgccg gctggccggg
     1801 ggagccggcg ctgggtccgc cggccttcat gcaccgcttc tcgggcatgg atctacccga
     1861 ggcacccctc ggccatcact ggctcgactc cacccacgtc accttcggcg tcctgaccct
     1921 gggctggatt cagggcgatg tgaagctgga gggctccgtc ttcaccggcc gggagccgga
     1981 cgaggaccgg ctcgacatcg aggagccgaa gatggactcc tggtccgtgc gggcgagctg
     2041 gcagccgaca ccggactggt cgctccaggc gagctacggt ttcctgaaga gccccgagca
     2101 actcgagccc gaggtggacc tggaccgcgt cacggcgtcc gccatctaca accggccgct
     2161 cccggacggc aactggcaga ccaccttcgc ctgggggagg aacgccaagg accccgggga
     2221 gaccacggac acctacctgc tggagtccac gctggccttc gcgccgcggc acaccgtctt
     2281 cggccgcgcc gagcgccagg agaacgacga gctgttcggc cacggcgacc atggacacga
     2341 gggcgaggcg tccgacgtcg tcgattctct cgagggccga gtcttcaacg tcggcaagct
     2401 cacgctgggc tacatccatg acctcttcgc gggcgatggc taccaggcgg gcatcggcgc
     2461 ctcgtggagc ctcgcgctgg tccccgagga gctggaggag gtctacggcg atcagccgtc
     2521 ctcctggatg gcgttcgtgc gggtggttct agggtcggaa atgtgatcgc tgtgatcagg
     2581 tgccctgcaa ctgcctccgc acctccggag cgagatcctc ccgcgcgagg agcctctcgc
     2641 gcagtggagg cttgctgcgg atggagcgga ggagcgagcc ccggtcctcc aggaagcggg
     2701 gatcggcgag ccaggcggcg aggaagctct cgacgggctc ggaacgtttc cgcagcaggt
     2761 ggaggaggag caggctgagg tcacgggagg gcagaggctc aagcgcctcc agcagcggcc
     2821 ccgtccactc gggattctcc tgaacggcgc ggaccaaggc ctccaaaagg agcttcttgc
     2881 ggttctcatc gttgcagccc gagaggaacc gcagggtggc cgcgggggtg gcagccagtg
     2941 ccctgccgag agcggacatc aggttccgga cctgcttctt cctctgggga cgggccagcc
     3001 aatcgagagc caggtccatc gcctcctcgt cgcccccgct ccgctccacc agccgcgcca
     3061 gcacccgctg tgccttcggc cacccttcgt tctctccgat ccaggccagg gcccagcggc
     3121 ggagctccgg gtgggtgggg ttggcgtcca ccagggggac gagcagccgg cggacattgg
     3181 gatggttggc atgggcccgc agccagtcca gcgcatcccg gacgagatcc gggtcggagg
     3241 gagacgcttc gaccagttgc accagcagct ccgccgtgcc ggtcgccccc tcgtggctcc
     3301 gcagccacgc cgccgcccgg ccgagaaccg ccgcgtcgcc cggccgcgcc gccaccagtc
     3361 gtgccagcag atgacccgtc ccggcgtagt ccgcggtggc accgatccgg tcgagcacgg
     3421 cgccgagggc gtcgggggaa tctttcaggg cgctcatcag catcgtcgcc cggccggggg
     3481 ccagatgcag gtccgccacc cggatcaccg agaggaggtc caccccggga ggcacggcgc
     3541 cgtcttttcc ataatggagc agggccagga cggcgagcca gctctggccg tccagtcgcg
     3601 cggcgcaggc ggcgagcccc acctccagac ggcgcgccgc cgcggcagcc ccgtggaggc
     3661 tgagcgccgc gtggaggagg cagaggttgc gggtctcgag cgtcccatcc agggcagccc
     3721 ggttccacag ctccaccaga cggtcccgga gaccgggctc gtccacccgg caggcgagga
     3781 tgcccaggtc gaccccggcc ggctgggccg cgagcgacgc ccgggcgacc cactccccga
     3841 cgtcgaaggc cgggacgggc cggcggtgcc aggcctcggc cgcgatgcgc ggatgggagg
     3901 cgccgatccc cgcgcccagg aaggcgaggt ggcgcgccgg ctcggtgacg gtgaagacct
     3961 cccagccctg ttcgctgacc agccgctgca gctcatagcg gcagctcggg gccagcgggt
     4021 cccggtggga gtggtccagg atcgccccga gcaccacctc cgggcaggtc atcccgaagc
     4081 gatgccagta gcagaccacc agataggcgc ggaccagcca cgccagctcg tcgggacgcg
     4141 tccgcgggtc gatgggccgg ccgtggttca gggtcatcca caggcggtcc accaggctct
     4201 tgaaggaagc gccggcggat gcctccatgg cgaggacgag gaagtcgtcc tcgcggtcct
     4261 ggaagagccc ggggtcacgg ccatggagga gagccagggc ctcccgctcc ggacgccgcg
     4321 gccggtcgag agggaagatc cggacgtcca cctgccggcc cagctggtga cggtgctcgt
     4381 ggaagaggta gtcggggctg gtggcgagga ccgccaaccg gcggctgcgc ggggccaggc
     4441 ggcgcagcag gtcgtcccag cccgactcgg cgaagagcgg gtcgtcgagc accagcagca
     4501 cggggagcgt cccctcttcc agccgcgcca gggagcgcag gagcgtgctg acatccccgt
     4561 cgtcgatccg ctccaggttg agcttggggg ccgccacgac cgccagcccc tcctggaccg
     4621 cccgcgccgc cacccgccgc accagggtgg tcttgcccgc tcccggcgct cccagcacga
     4681 agagcgccgg cagcacctcg tcatcccccg cgctctccag cagctcgccg acctgggaga
     4741 agaggcgctc cgtcggctcc cgttccagaa agtgggtctc ggggctctgg ccgtg
//
LOCUS       DASPJI010000753         4714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_504597, whole genome shotgun sequence.
ACCESSION   DASPJI010000753 DASPJI010000000
VERSION     DASPJI010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504597"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4704)
                     /locus_tag="VEL74_21170"
     CDS             complement(<1..4704)
                     /locus_tag="VEL74_21170"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65107.1"
                     /translation="MRTVFPAPEGEPVQRVLAEIPSIVSTLSSPLEAEAWRPFDLERG
                     PLLRVVIEGPVVLFVVHHLVADFWSLALAARELSALYLEESGGVAAALPALQLSYLDF
                     THWQEEALAGARGEALLEWWRERLAGLPDLDLPTDRPRPPIQTWRGAGQAAVLPPALA
                     DGVRVLASRHGATLFTALLAAFQAQLGRYTGQEDLAVGAPTAGRSAPELAGLVGYFVN
                     PVVLRADLAGEPPFTDVLARARETVLTALARADLPFALLAERLRPVRDPARPPLFQAM
                     LVLQAGRPQDDPGLAAFALGEAGARIGLGGLVLESLPLAERRAQFELILRLAEETSGG
                     GLLVSLEHNADLFDTATAARMVEHFRTLLAGAVAGPDRCWADLPLLTEDERRQILGEW
                     SGAGPRPEDTDATLSGLFEAWVARTPQAPALVFEGESVSYGDLDARAERLAGRLRRLG
                     VGPEVVVGLCAERSIEMVVGILGVLKAGGAWLPLDPALPRERLAQLLADAEVPVLLAK
                     AAVLPGLPPMEGESTRLLLLDSADEPEEIPASRTSSATADNLAYVLYTSGSTGAPKAV
                     AVPHRGVANRLLRAREAYGIRPGDAMLQRAAGGFDVSVWEIFGPLSSGARVVLARPGG
                     QRDPRYLARLLDEQEVTLVNFTPSALDALLDEEGIAERAASLRQVFVGAEALPRDLPV
                     RFFAAGLRAPLDNMYGPTEASIDLLWYSCRPDARLPEGSTVPIGRPIPGTRVHVLDAR
                     LAPVPAGIPGELWLGGVGLARGYRGRPDLTAERFLPDPLPTKPGERLYRTGDLARWLP
                     GGDLEFLGRVDLQVKVRGVRVEPGEVEAVLRGRPGVREAAVAPRADATGRQGLVAYVV
                     WDAPSVLDARELRSFLAARLPEAMVPAAFLPVPELPRTPSGKLDRRALASAISGLFTT
                     PPAGDGGLHTPPRTPVEELLAGLWSELLGAERIGVRDDFFELGGHSLLATRMLARVSR
                     LFGVELPVSAAFQFPTLAALAEQIEAALGAASAPPLRAVARDRFPGGAPLSFAQRRLW
                     FLDRLQPGSPVYNLPGAVRLTGPLDLAALASALSGVARRHESLRTVFRLEGDEPVQVI
                     GSPSTVASAPPPLVDLAGLTEGLRNAEAEWLAGREAIRPFELAAGPLCRVSLLRLASG
                     DHLLLVNLHHSVADGWSLGIFLRELAALYEGVPLPELPVQYADYAVWQREWMQGEALD
                     SQLAYWRRQLADLPVVELPADRPRPAIRRLRGGVRSALLPAGLADAVEGIARGRGATL
                     FMELLAAFQILLRRYTGEVSVPVGSPVANRQRPEVEGLIGFFANTLVLRASMADDPPF
                     DTLLARVREACLEAYAHQDLPFERLVEELRPGRDLSQTPLFQVMLVLEEPLPRPRAGA
                     LELRPERIETETAKFDLTLTAAPVEDGRLVSALEYDSALFDPVTVDRMLGHWRTLLAG
                     IVADPAAPVSALPLLQEAETDQVLRQWNATGAEERPDLCLHELFAEQARRTPTAEALV
                     ASDEEGDLRLTYQELSARAGRLAQRLRRLGVEPEVRVGVCLERSADLVVSLLAVLEAG
                     GAYVPLDPAYPRERLE"
BASE COUNT          741 a         1750 c         1719 g          504 t
ORIGIN      
        1 ttccagccgc tcgcgagggt aggcggggtc cagcggcacg taggcgccgc cggcctcgag
       61 caccgcgagc agggagacca ccagatccgc cgagcgctcc aggcagacgc ccacccgcac
      121 ctcgggttcc accccaagcc gccgcagccg ctgagccagg cggccggcgc gcgcgctcag
      181 ctcctggtag gtcaaccgca ggtccccctc ctcgtccgac gcgacgagcg cctcggccgt
      241 gggggtacgg cgcgcctgct ccgcgaacag ctcatggagg cagaggtccg ggcgctcctc
      301 cgcgccagtg gcgttccact gccgcagcac ctggtccgtc tcggcctcct gcagcagcgg
      361 cagggccgag accggcgccg cgggatcggc gacgatccca gcgagcagcg tccgccagtg
      421 ccccagcatg cgatccaccg tcaccggatc gaagagggcg gagtcgtact ccagcgccga
      481 gacgagccgg ccgtcctcca ccggggccgc cgtcagggtc agatcgaatt tggcggtctc
      541 cgtctcgatc cgttcgggcc tgagctccag ggctccggca cgcggccgcg gcagcggctc
      601 ctccaggacc agcatcacct ggaagagcgg cgtctgcgac aggtcccgcc caggccgcag
      661 ctcctcgacc agccgctcga aggggaggtc ctggtgcgcg taggcctcga ggcaggcctc
      721 ccgcacccgc gccagcagcg tgtcgaaagg gggatcgtcg gccatcgagg cgcgcagcac
      781 cagggtgttg gcgaagaagc cgatcagccc ctccacttca ggacgctggc ggttggccac
      841 cggcgagccc accggaaccg aaacctcgcc cgtgtagcgc cggaggagga tctggaaggc
      901 ggcaaggagc tccatgaaca gggtcgcgcc gcgaccgcgg gcgatcccct ccaccgcgtc
      961 cgccagcccg gccggcagca gcgccgaccg cactccaccg cgcagccggc ggatggcggg
     1021 gcgcggccgg tcggccggca gctccaccac cgggaggtcg gcgagttggc ggcgccagta
     1081 ggcgagctgg ctgtcgaggg cctccccctg catccactcg cgctgccaga ccgcgtagtc
     1141 ggcgtactga accggcagct cagggagcgg caccccctcg tagagcgccg ccagctcccg
     1201 caggaagatg cccagcgacc agccgtcagc caccgagtga tggaggttga ccagcagcag
     1261 gtgatccccg gatgcgagcc gcagcaggga gacccggcag agcggccccg ccgccagctc
     1321 gaaggggcgg atcgcctcgc gcccggcgag ccactccgcc tccgcgtttc ggagtccttc
     1381 ggtcaggccc gcaaggtcga cgagcggcgg tggcgccgat gccacggtgg agggagagcc
     1441 gatcacctgg accggttcgt cgccttcgag ccggaacacg gtacggagcg actcgtgccg
     1501 ccgcgccacc ccggagaggg cggatgccag cgccgcgagg tccagcggac cggttagccg
     1561 gaccgcgccc ggcaggttgt agaccgggct cccgggctgc aacctgtcga ggaaccagag
     1621 gcggcgttga gcgaaggaga ggggggcacc gccggggaaa cggtcccggg cgacggcgcg
     1681 cagcggcggc gccgacgccg cccccagagc ggcttcgatc tgctccgcca gggccgcgag
     1741 ggtgggaaac tggaaggcgg cgctcaccgg cagctccacg ccgaaaaggc gggagacccg
     1801 cgccagcatc cgcgtggcga gcagggagtg cccacccagc tcgaagaagt cgtcccggac
     1861 gccgatccgc tcggccccga gcagctcgct ccagagtccc gccagcagct cctcgaccgg
     1921 agtgcgcggt ggagtgtgga gcccgccgtc gccggcaggg ggggtggtga agagacccga
     1981 tatcgcactg gcgagggccc ggcggtccag cttgccgctc ggcgtgcgcg gcagctccgg
     2041 caccggcagg aaagcggccg ggaccatcgc ctccggcagc cgcgccgcga ggaagctccg
     2101 cagctcgcgc gcgtcgagga ccgacggcgc gtcccacacc acataggcca cgagaccctg
     2161 ccggccggtg gcgtcggccc gcggcgccac cgcggcctcg cgtactccgg ggcggccgcg
     2221 cagcaccgcc tccacctcgc cgggctcgac gcgaacgccg cgcaccttga cctgcaggtc
     2281 gactcggccg aggaattcca ggtcgccgcc gggcagccag cgggcgagat cccccgtacg
     2341 gtacaggcgc tcgcccggtt tcgtcggcaa ggggtccggc aggaacctct cggcggtcag
     2401 gtccggacgg ccgcggtagc cgcgggccag gccgacccct cccagccaca gctccccggg
     2461 gatgccggca ggaaccggtg ccaagcgggc gtccagcacg tggacgcggg tgccggggat
     2521 ggggcgcccg atggggaccg tgctcccctc gggaagacgg gcatccggac ggcaggagta
     2581 ccagagcagg tcgatggagg cctcggtagg cccgtacatg ttgtccagcg gcgcccgcag
     2641 gccggccgcg aaaaagcgga ccggcaggtc gcgcggcagg gcctcggcgc caacgaacac
     2701 ctggcgcagg gaggcggcgc gctcggcgat gccctcctcg tcgagaaggg catccagcgc
     2761 cgacggggtg aagttgacga gcgtcacctc ctgctcgtcc aggaggcgcg ccaggtagcg
     2821 cgggtcccgc tgcccgccgg gacgggccag caccacccgc gcaccggacg agagagggcc
     2881 gaagatctcc cacacggaaa catcgaagcc gccagccgcc cgctgcagca tggcgtctcc
     2941 tggccggatg ccgtaagcct cccgcgcccg gagaagccgg ttggccaccc cgcggtgcgg
     3001 caccgccacc gccttcggtg cgccggtaga gcccgaggtg tagagcacgt aggccaggtt
     3061 gtccgcggtg gccgacgagg tccgcgaggc ggggatctcc tccggctcgt ccgcgctatc
     3121 caggaggagc aggcgggtgc tctcaccctc catagggggc agaccgggga gcaccgccgc
     3181 cttggcgagg agcaccggca cctccgcgtc ggccagcagt tgcgccaggc gctcccgcgg
     3241 gagcgcggga tcgagcggca gccaggcgcc gccggccttg agcacgccga ggatccccac
     3301 caccatctcg atggaacgct cggcacagag gcccaccacc acttccggtc ctacgccgag
     3361 gcgacgcagc cggcccgcca gacgctccgc ccgcgcgtcg aggtcgccgt agctcaccga
     3421 ctccccctcg aacaccaggg ccggagcctg cggcgtcctc gccacccagg cttcgaacag
     3481 cccgctcagc gtggcgtcgg tgtcctccgg ccgcgggccg gcccctgacc actcgcccag
     3541 gatctggcgg cgctcgtctt cagtcaggag gggcaggtcc gcccagcaac ggtcagggcc
     3601 ggctaccgcg cccgcgagga gggtgcggaa gtgctccacc atgcgggccg cggtggcggt
     3661 gtcgaagagg tcggcgttgt gctccagaga gacgaggaga ccgccgccgg aggtctcttc
     3721 cgcgaggcgc aggatcagct cgaactgtgc ccgccgctcg gccaggggga gcgattcgag
     3781 aaccagacct cccagcccga tgcgggcgcc ggcctctcca agagcgaaag cggcgagccc
     3841 gggatcgtcc tgcggccggc cggcctgcag cacgagcatg gcctggaaga gcggcgggcg
     3901 tgccggatcg cgcaccggcc ggagccgctc ggcgagcagg gcaaagggga gatcggcgcg
     3961 cgccagggcg gtcagcaccg tctcgcgggc ccgcgccagg acatcggtga acggcggctc
     4021 gcccgcgagg tcggcgcgca gcaccaccgg attgacgaaa tagccgacga ggcccgccag
     4081 ctcgggagcg ctccggcccg cggtgggagc ccccacggcc agatcctcct gcccggtgta
     4141 gcgcccgagc tgggcctgga acgcggcgag cagcgcggtg aagagcgtgg ccccatgccg
     4201 gctcgcgagg acgcggacgc catcggccag ggccggcggc agcaccgcgg cctggccggc
     4261 gccccgccat gtctggatgg gcggtcgcgg ccggtcggtg ggcaagtcca ggtccggcag
     4321 gcccgccaga cgctcccgcc accactcaag gagcgcctcg ccccgcgcgc cggcgagggc
     4381 ctcctcctgc cagtgggtga agtccaggta ggacagctgc agagctggga gagccgctgc
     4441 cactccgccg ctctcctcca ggtagagcgc cgacagctcc cgcgccgcca acgccagcga
     4501 ccagaaatcg gcaacgaggt ggtgcaccac gaagagcacc accggaccct cgatcaccac
     4561 ccgcaggagc ggcccgcgct cgaggtcgaa cggccgccaa gcctcggctt ccaagggaga
     4621 cgacaatgta gaaacgatgg acgggatctc ggcgagcacc cgctgcaccg gctcgccctc
     4681 gggagccggg aacacggtcc gcaacgcctc gtgg
//
LOCUS       DASPJI010000754         7359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_105386, whole genome shotgun sequence.
ACCESSION   DASPJI010000754 DASPJI010000000
VERSION     DASPJI010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7359)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7359
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105386"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="VEL74_21175"
     CDS             complement(<1..1515)
                     /locus_tag="VEL74_21175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65108.1"
                     /translation="MSATLIPASGAWAAGGWTGGQDFYHVPLSWCIVQGTPAHVSPNV
                     AGDFATDDLIWRRHERPSDHIYINQAGITFRSAINDIWGQLNFPIIPDPDLNFGVQGD
                     MRGEDVNTFGAEFNAMLSSCETAWDNLGRSGIGITAVNAGVFHDGNGQYVGTIGWGGC
                     VESQFTGLCIPPYEGVITVVDNHYLHPSVPNRTLPNSTLQFILTDPMDQLVGHELGHA
                     LSLPHRTNNTALMNPGPVDNNSDGMSDNITLNATEVNAVRASAVLVPGLEIDPPRLIL
                     PGNVRAQRRPDKVKEHAGRPAHLDLASVKVTGDVAQREIAVTQQLFGLLPATAKQFHW
                     FLIDTDDAVTGAGPAAIRKAGGPVTQFTGADIILRADVAGQAVTGGQAWSFQAGNLVP
                     LVGVGFDLRRLVMHPLRTKIVNRDGAVVWAPPSEGHGEFPVHHIIAATLPGEKLGALL
                     EGKPFRVHAVIVTDPEKGEAVDEIEAKDPERGTVFDLKLPSFPHCFAQGETTPGG"
     gene            1743..2153
                     /locus_tag="VEL74_21180"
     CDS             1743..2153
                     /locus_tag="VEL74_21180"
                     /inference="COORDINATES: protein motif:HMM:NF021119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HYG65109.1"
                     /translation="MTRFDVHQGGGTGGSKDKDAGPITLFERLADHLSYTGRATTVFG
                     EAIERDGVTVIPVAKAKWALGGGGGHRRPGNQEGMGGGGAVNVAPVGYIEIRNGESRF
                     RPIWDPVTTFAASVAAGAALVFLLSRLRGKDEEE"
     gene            2150..2659
                     /locus_tag="VEL74_21185"
     CDS             2150..2659
                     /locus_tag="VEL74_21185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65110.1"
                     /translation="MRAAAAVLVAVAAVSVLAGACAAPAPPPPVPLRGEAADLSRLAG
                     EWNGEYTSASSGRSGSIRMTLEAATPEMAFGDVLMFPARPEERPEAVNPERGDAGPQA
                     LAIRFVSVTGDSVSGTLEPYRDPACACVVSTTFSGRVRGDVIEGTFVSHGGAGYLPNE
                     GRWRVDRKK"
     gene            2752..3360
                     /locus_tag="VEL74_21190"
     CDS             2752..3360
                     /locus_tag="VEL74_21190"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HYG65111.1"
                     /translation="MDDGAPSSRRDEILEQAFELVREAGLTGLTMKKVAKRVGVTEPA
                     LYRHFPTKQAILVGIAKRLAEKLLGPIRAIAAEEETPPEQRVERILTHHFKLVMETNG
                     LPFLLMAEASVSGDQDVADTLGGAMLSYQQIMVGILETMPRTPGMPEPRHMLPMLVGV
                     IAAVAIQRRALPCLPVDPIAVLELIPYLVRRLTSPEPGEEPA"
     gene            3357..4721
                     /locus_tag="VEL74_21195"
     CDS             3357..4721
                     /locus_tag="VEL74_21195"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HYG65112.1"
                     /translation="MRSLVSLTLVVLTLVLPAGATAQETAQEPARLTLAEAVERALAR
                     YPGVGAARARQEQAREAITEAKASRRPAGRLNATGTQYEEPMVVTPIHGFGPGLFPEF
                     DTTVFQGTLTVSYTLWDGGATEARIRSAGEQAGVAGAALGAAEQALARRVAASYLTAQ
                     GQSQVLAAHDRRLAALQGELSRARQRFDAGKAAQVEVIRAEAAVAAAEAQRVALAAAV
                     GNAERDLARLLDAPLDEVRAGRLAPVTLAGPALPPREALVADGLAVSPAVEQARRQVE
                     ASRAAEALARSAYRPTLRAVGNYNEWASSEGDFTGEWNAGFQFAVPLFDFGVTRSRVA
                     QSAAATRAAAEQLRLAELQVREEVDRAAADTEQALAQVASLEKAVERFAEVVRVQKLL
                     LDTGAGTQIDYLNAEAELLAARASLATAENAAALARMDLARATGRLGPEWLRQNLRQT
                     ETTP"
     gene            4718..5725
                     /locus_tag="VEL74_21200"
     CDS             4718..5725
                     /locus_tag="VEL74_21200"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HYG65113.1"
                     /translation="MSPKKVVPPILLLGAIGAGIWWYTTSRTAEAEDGLAASGTVEAT
                     EAQLGFQVPGQISALDVREGDRVRAGQVLARLDPVEAEARRAQVAAQVAGARAQLAEL
                     EAGSRREEVAQARAALDVAQDREADARRDLERNRMLFDGGAIPREALDKSRLAVEVAG
                     SQRTQAAEQLRLVQAGPRRERVDAARAQLAQAEAALRASEASLENLTLTAPFDGVVTV
                     RHREPGEIVVAGAPVLTLMDPGSRWVRIYIKEDRLGEVRIGAPAAIRSDTFPDKRYQG
                     QVSFIASEAEFTPKSVQTTEERVRLVYEVKVRVTGDPANELKPGLPADVTLETAKGRE
                     G"
     gene            5725..6669
                     /locus_tag="VEL74_21205"
     CDS             5725..6669
                     /locus_tag="VEL74_21205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG65114.1"
                     /translation="MPDPMIRAEGLTRRFGELTAVDGLSFEVRPGELFGLVGPDGAGK
                     TTTLRMLAGVLRPTSGDALVAGVSMARHPESAKPRIAYMSQRFGLYTDLTVQENLAFY
                     ADLHRVPRAERAARFERLYRFSNLGPFKDRLAGKLSGGMKQKLGLSCALIHQPEILLL
                     DEPTFGVDPISRRDLWRIVHERVGQGTTAVVSTAYMDEAERFDRLVLLHRGRVLAVDT
                     PESLQRGYAGEVLEIPVDRPREALETVRHHPAARRAALFGEHLHLTVDDAATAGPALS
                     GALSQAGFRPGEARRILPSLEDVFIERIASADEQEAAA"
     gene            6666..>7359
                     /locus_tag="VEL74_21210"
     CDS             6666..>7359
                     /locus_tag="VEL74_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYG65115.1"
                     /translation="MSAPGDDRAVEVEDLTRVYGQFVAVDRVTFSVRRQEVFGFLGPN
                     GAGKTTTIRMLATLIKPDAGTARVLGHDIVTESDAVRAGVSLTGQLASVDEDLTGREN
                     LVLIGRLLGLGRAAAKDRATELLDAFGLAEAAGKLVKHYSGGMRRRLDIAASIVVTPR
                     VMFLDEPTTGLDPRSRGQVWEIIRALVAEGTPAQLKASVGSGSLRIRLINPEQRGEAE
                     QILAREFGTVHLE"
BASE COUNT         1013 a         2641 c         2682 g         1023 t
ORIGIN      
        1 gccgcccggg gtcgtctcgc cctgcgcgaa gcagtgcggg aacgagggga gcttgaggtc
       61 gaagacggtg ccgcgctcgg gatccttggc ttcgatctcg tccacggctt cacccttctc
      121 cgggtccgtg acgatcacgg cgtggacacg gaacggcttg ccttcgagca gggcgccgag
      181 cttctcgccc ggcagcgtgg cggcgatgat gtggtgcacg gggaactcgc cgtgcccctc
      241 gctcggcggc gcccagacca ccgcaccgtc ccggttgacg atcttcgtgc ggagcgggtg
      301 catgaccagg cggcgcaggt cgaagccgac gcccaccagg gggaccaggt tcccggcctg
      361 gaagctccag gcctggccgc cggtgaccgc ctggccggcg acgtccgccc gcaggatgat
      421 gtcggcgccg gtgaattggg tgacgggccc gcccgccttg cggatggcgg cgggtccggc
      481 gccggtcacc gcgtcgtcgg tgtcgatcag gaaccagtgg aactgcttgg cggtcgccgg
      541 cagcaggccg aagagctgct gggtgacggc gatctcacgc tgcgccacgt cgccggtcac
      601 cttgaccgaa gccaggtcga ggtgggccgg ccggcccgcg tgctccttca ccttgtccgg
      661 ccggcgctgg gcgcggacgt tgccaggcag gatgagccgg ggcgggtcga tctcgaggcc
      721 gggaacgagc accgcggagg cgcggacggc gttgacctcg gtggcgttga gggtgatgtt
      781 gtcggacatg ccgtcgctgt tgttgtccac cgggccgggg ttcatgagcg cggtgttgtt
      841 cgtccggtgc ggcagcgaca gcgcgtggcc gagctcgtgg ccgacgagct ggtccatcgg
      901 gtcggtcagg atgaactgca gggtgctgtt ggggagggtc cggttgggca cgctcgggtg
      961 gagatagtgg ttgtcgacga ccgtgatcac accttcgtac gggggaatgc acaggccggt
     1021 gaactggctc tcgacacagc cgccccagcc gatggtgccg acgtactgcc cgttgccgtc
     1081 atggaagacg cccgcgttca ccgcggtgat gccgatcccg gagcggccga ggttgtccca
     1141 ggcggtctcg caggagctga gcatcgcgtt gaactcggcg ccgaaggtgt tgacatcctc
     1201 accccgcatg tcgccctgca ccccaaagtt gaggtccggg tcggggatga tggggaagtt
     1261 gagctgcccc cagatgtcgt tgatggcgga ccggaaggtg atcccggcct ggttgatgta
     1321 gatgtggtcg gacggccgct cgtgacgccg ccagatcagg tcgtcggtgg cgaagtctcc
     1381 ggcgacgttc gggctgacgt gggccggcgt cccctgcacg atgcaccagg acagcggcac
     1441 gtggtagaag tcctggcctc cggtccagcc gccggcggcc cacgcccccg aggcggggat
     1501 cagggtggcc gacatgatac ctagggcgac actccatgct ctcactcgca tccactctcc
     1561 tcctaattgg tagggcccag caatgaggag gagttctaca ggaatttcat cggttcggct
     1621 tcttccatat tgctattttt ggctccggct cccttctgtg acatctccgc ctccccactg
     1681 atccgggtcg ttccggccgg tccggctggt gctacattcc agggaacgga aggaggagtg
     1741 ccatgacgag gttcgatgtg catcaggggg gcgggaccgg ggggagcaaa gacaaggacg
     1801 cggggccgat caccctgttc gagcggctgg cggatcacct gagctacacc ggccgggcca
     1861 ccacggtctt cggcgaggcg atcgagcgcg acggggtcac cgtgatcccg gtggccaagg
     1921 ccaagtgggc gctcggcggc ggtggcggac accggcggcc tggcaaccag gaggggatgg
     1981 ggggcggcgg tgctgtcaac gtcgcgccgg tgggctatat cgagatcagg aacggggagt
     2041 cgcgcttccg gcccatctgg gacccggtga cgacgttcgc ggcgagcgtg gccgccggcg
     2101 ccgccctggt cttcctgctg agccgcctgc gcggcaagga cgaggaggag tgagagccgc
     2161 ggctgcggtc ctcgtcgcag tcgcggccgt atcagtcctc gcgggggcgt gcgcggcgcc
     2221 cgcgcccccg cctcccgtgc cgctccgcgg cgaggccgcc gacctctccc ggctggccgg
     2281 ggagtggaac ggcgagtaca ccagcgcctc cagcggccgg agcggctcga tccggatgac
     2341 cctcgaagcg gccactcccg agatggcctt cggcgacgtc ctcatgttcc ccgctcgccc
     2401 cgaggagcgg cccgaggcgg tcaaccccga gcggggcgac gccgggcccc aggcgctcgc
     2461 gatccgcttc gtctccgtga ccggcgactc ggtgagcggc acccttgagc cctaccgtga
     2521 ccccgcctgc gcctgcgtcg tctccaccac cttcagcggc cgcgttcgcg gagacgtcat
     2581 cgagggcacc ttcgtctctc atggtggcgc cgggtatctg ccgaacgaag gacggtggcg
     2641 ggtggaccgg aagaagtagg cctcccggtg aggggttgac ggcccgccct ccgtttccta
     2701 tcctggagac gttagtgagt cctcactaac gttgaatcgg gagggtagga gatggacgat
     2761 ggcgcgccga gctcacggcg ggacgagatc ctggagcagg cgttcgagct ggtccgggag
     2821 gcggggctca ccgggctcac catgaagaag gtggccaagc gggtgggcgt caccgagccc
     2881 gccctctacc ggcacttccc gaccaagcag gcgatccttg tcggcatcgc caagcggctc
     2941 gccgagaagc tcctcggacc gatccgcgcc atcgccgccg aggaggagac tccccccgag
     3001 cagcgggtcg agcgcatcct cacccatcac ttcaagctgg tgatggagac caacggcctg
     3061 cccttcctcc tgatggccga ggcttcggtc tccggcgacc aggacgtggc cgacaccctg
     3121 ggcggggcaa tgctgagcta ccagcagatc atggtcggga ttctggagac gatgccccgc
     3181 acgcccggca tgccggagcc gcggcacatg cttcccatgc tggtgggcgt catcgccgcg
     3241 gtggccatcc agcgccgcgc cctgccgtgc ctgcccgtcg acccgatcgc ggtgctcgag
     3301 ctgatcccct acctggtgcg gcggctcacc agtcccgaac cgggggagga acccgcatga
     3361 gatccctggt atccctgacg ctggtggtcc tgacgctggt tctcccggct ggcgccacgg
     3421 cccaagagac ggcgcaggag ccggcacgcc tcaccctggc cgaagccgtg gagcgtgccc
     3481 tcgcccgcta tcccggggtg ggcgccgccc gcgcccgcca ggagcaggcg cgcgaggcga
     3541 tcaccgaggc caaggcgtcc cgccgtcccg ccgggcggct caacgcgacc ggtacgcaat
     3601 acgaggagcc gatggtggtc acccccatcc acggcttcgg gcccggcctc ttccccgagt
     3661 tcgacaccac ggtcttccag gggactctca cggtgagcta caccctctgg gacggcggcg
     3721 ccaccgaggc gcggatccgc tcggcgggcg agcaggccgg ggtggcgggg gcggcgctgg
     3781 gcgcggccga gcaggccctg gcgcgtcgcg tcgccgcctc ctatctcacg gcccaggggc
     3841 agagccaggt gctcgccgcc cacgaccggc ggctcgcggc gctccagggg gagctctccc
     3901 gcgcccggca gcgcttcgac gccggcaagg ccgcgcaggt ggaggtgatc cgcgccgagg
     3961 ccgccgtcgc cgccgccgag gcgcagcggg tggcgctcgc cgccgcggtg ggcaacgccg
     4021 agcgcgacct cgcccgcctg ctcgacgcgc cgctcgacga ggtgcgcgcc ggccgcctgg
     4081 ccccggtgac cctggccggt cccgcgctgc cgccgcgcga ggcgctggtg gcggacgggc
     4141 tcgcggtcag cccggcggtg gagcaggcgc gccgccaggt ggaggcctcg cgggcggccg
     4201 aggcgctggc ccggagcgcc taccggccga cgctgcgggc ggtgggcaat tacaacgagt
     4261 gggcctcgtc cgagggggac ttcaccggcg agtggaacgc cggcttccag ttcgcggtcc
     4321 ccctcttcga cttcggcgtc acccgcagcc gggtggcgca gtccgcggcc gccacccgcg
     4381 ccgccgccga gcagctccgc ctcgccgagc tgcaggtgcg cgaggaggtg gaccgcgccg
     4441 ccgccgacac cgagcaggcg ctggcccagg tggcgagcct ggagaaggcg gtggagcgtt
     4501 tcgccgaggt ggtgcgggtc cagaagctcc tcctggacac cggcgccggc acccagatcg
     4561 actacctcaa cgccgaggcc gagctgctgg ccgcccgggc cagcctcgcc acggcggaga
     4621 acgccgcggc gttggcgcgc atggacctgg cgcgcgccac cggccggctc ggccccgagt
     4681 ggctgcgcca aaacctgcgg cagacggaga ccaccccatg agccccaaga aggtcgtccc
     4741 cccgattctc ctgctgggtg ccatcggcgc cggcatctgg tggtacacca cgtcccggac
     4801 cgccgaggcc gaggacggcc tcgccgcctc cggcaccgtc gaagccaccg aggcccagct
     4861 cggcttccag gtgcccgggc agatctcggc cctcgacgtg cgcgaggggg accgggtgcg
     4921 cgccgggcag gtgctggcgc ggctcgatcc ggtggaggcc gaggcccggc gggcccaggt
     4981 ggccgcccag gtggccggcg cccgcgccca gctcgccgag ctcgaggccg gctcccgccg
     5041 cgaggaggtc gcccaggccc gcgccgccct cgacgtcgcc caggaccggg aggccgacgc
     5101 ccgccgcgac ctggagcgca accgcatgct gttcgacggc ggcgccatcc cccgggaggc
     5161 gctggacaag tcgcgcctgg cggtggaggt cgccggcagc cagcgcaccc aggcggccga
     5221 gcagctccgc ctggtccagg ccggcccgcg ccgcgagcgc gtcgacgccg cccgcgccca
     5281 gctcgcccag gccgaggccg ccctgcgcgc ctccgaggcg tcgctagaaa acctcaccct
     5341 gaccgccccg ttcgacggcg tggtcaccgt ccgccaccgc gagccgggcg agatcgtggt
     5401 cgccggcgcg ccggtgctca ccctgatgga cccgggcagc cgctgggtgc gcatctatat
     5461 caaggaagac cgcctcggcg aggtccgcat cggcgccccg gccgccatcc gctcggacac
     5521 cttcccggac aagcgctatc aaggccaggt ctccttcatc gcctccgaag cggagttcac
     5581 cccgaagagc gtccagacca ccgaggagcg ggtgcgcctc gtctatgagg tcaaggtccg
     5641 cgtcaccggc gatcccgcga acgagctcaa gcccgggctg ccggccgatg tgacgctgga
     5701 gacggcgaag ggcagggagg ggtgatgccc gatcccatga tccgcgccga ggggctgacc
     5761 cgccgcttcg gcgagctgac ggcggtggac ggcctgtcgt tcgaggtccg gccgggcgag
     5821 ctgttcggcc tggtggggcc cgacggcgcg ggcaagacga cgacgctccg catgctcgcc
     5881 ggcgtcctcc gtcccacctc cggcgacgcc ctggtggccg gggtcagcat ggcccgccac
     5941 ccggagagcg ccaagccgcg catcgcctac atgtcgcagc gcttcgggct ctacacggac
     6001 ctcaccgtgc aggagaacct ggccttctat gccgacctcc accgggtgcc gcgcgccgag
     6061 cgggcggccc gcttcgagcg cctctatcgc ttctcgaacc tgggcccgtt caaggaccgc
     6121 ctggcgggga agctctccgg cgggatgaag cagaagctcg gcctctcgtg cgccctgatc
     6181 caccagccgg agatcctcct gctcgacgag cccaccttcg gcgtcgatcc catctcgcgg
     6241 cgcgacctct ggcgcatcgt ccacgagcgg gtggggcagg ggaccacggc ggtcgtcagc
     6301 accgcctaca tggacgaggc cgagcgcttc gaccgcctcg tgctgctcca ccgcgggcgc
     6361 gtcctggcgg tggacacacc cgagtccctc cagcgcggct acgccgggga ggtgctggag
     6421 atcccggtgg accgcccgcg ggaggcgctg gagacggtgc ggcaccatcc ggcggcgcgc
     6481 cgggcggcgc tcttcggcga gcacctccat ctcacggtgg acgatgccgc gacggccggg
     6541 ccggcgctct cgggcgccct gagccaggcc ggcttccggc ccggcgaggc gcggcgcatc
     6601 cttccctccc tcgaagacgt cttcatcgag cggatcgcca gcgcggacga gcaggaggcc
     6661 gcggcgtgag cgctcccgga gacgaccggg cggtcgaggt ggaggacctg acccgcgtct
     6721 atggccaatt cgtggccgtg gaccgggtca ccttctccgt gcgccgccag gaggtcttcg
     6781 gcttcctcgg ccccaacggc gcgggcaaga cgacgaccat ccgcatgctg gccaccctga
     6841 tcaagcccga cgccggcacc gctcgggtcc tggggcacga catcgtgacc gagagcgacg
     6901 cggtccgcgc cggcgtgagc ctcaccggcc agctcgcctc ggtcgacgag gacctcaccg
     6961 gccgggagaa cctcgtcctc atcggccggc tgctgggcct gggccgagcg gccgcgaagg
     7021 accgtgcgac cgagctgctc gacgccttcg ggctggccga ggccgccggc aagctcgtga
     7081 agcactactc cggcgggatg cgccgccggc tcgacatcgc cgcgagcatc gtcgtgaccc
     7141 cgcgcgtgat gttcctcgac gagcccacta ccgggctcga cccccgctcg cgcggccagg
     7201 tctgggagat catccgcgcg ctcgtggccg agggcacccc cgcccagctc aaggcgtcgg
     7261 tcgggagcgg cagcctgcgc atccggctga tcaacccgga gcagcgcggc gaggccgagc
     7321 agatcctcgc gcgcgagttc ggcacggtgc atctcgagg
//
LOCUS       DASPJI010000755         6377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_48670, whole genome shotgun sequence.
ACCESSION   DASPJI010000755 DASPJI010000000
VERSION     DASPJI010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48670"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="VEL74_21215"
     CDS             <1..1473
                     /locus_tag="VEL74_21215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HYG65116.1"
                     /translation="DRTVIAIPLLDEFRAERDAAEAARKKRRRAPEARLYDVIIDLNL
                     DYPGGREEAKKTVRDWIQKAIDGVGTRPGQGVHQVKSEITNQYLFARLERRVVEELVR
                     INHGEGQPEKAPRRALFRVWPDFKVGPLLTRSLATVKADAARVSFSAQGKDILWAVLD
                     SGIQKEHPHFQKHGNLDLPAPLKHRDFTALDESSEQPLVDRFGHGTHVAGIVAGELTG
                     QVRTVTRHRDEQGEVSYQTSELDAISGVAPLCKLLSLKVLDDDGNGLASNLIAAIALI
                     QEANGHGRRIVVHGVNMSVGYDFEPEWFACGQSPLCVEVDRLVRSGVAVVVAAGNTGY
                     GFAQSAFRGTVAAGMNLTINDPGNAELAITVGSTHRDSPHIYGVSYFSSKGPTGDGRA
                     KPDLVAPGEKIVSCAAGKPREELREKAADCDYREESGTSMAAPHVSGAIAAFLSIRRE
                     FIGQPEKVKAIFMASATDLNRERYFQGRGLVDLMRAIQSV"
     gene            1490..2827
                     /locus_tag="VEL74_21220"
     CDS             1490..2827
                     /locus_tag="VEL74_21220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65117.1"
                     /translation="MAAISGFPYFEIEFDKKGAAHDPAQRQALLDFVAKGKDQVTDLL
                     VLSHGWNNDMADARDLYRRLLARLRDGIDGGKVAGVKERKFAICAVLWPSKKFTDKEL
                     IPSGAASAGGVVSTQALQEEIDRVKDAFDSPTAAADLDRARALVSKLEDSKAARDEFA
                     QILRSVLSAGTGNSNDEETPSAFFTLPGNEIIQRLSKPLPLAPGEPGGGAAGGVSGGT
                     GGPAGGAAALGIFSGIKGGAMKLLNLTTYYQMKERAGLVGSKGVNPILREVETKRPDL
                     KLHLIGHSFGGRLVTAAVLGPDGATPIKPETLTLLQAAFSHYGFSQGYEPGKDGFFRK
                     VVSEKRVAGPVLATCTHNDKAVGKAYPIASQIAGQVAAALGDKNSRWGGIGANGAQKT
                     PESVDGKLLPAGGAYSFTAGKVHNLNADSFIKDHSDIARDEVAHAVLTAVAAT"
     gene            complement(2833..4365)
                     /locus_tag="VEL74_21225"
     CDS             complement(2833..4365)
                     /locus_tag="VEL74_21225"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HYG65118.1"
                     /translation="MSRPRRGLLAFGLILAALAVLVPGLAVARPGGGEGYSGGGDSGG
                     GGGGDGDSGFVWLLLRLWIEFCIAYPHIGIPITIAVLLYLHFKRKKKAKAGPQNWDAA
                     PSHAHPPAGPAARPRSLDLEAIRKLDPDFSVVLFEDFAYALYARAYQAHASRRDLDAL
                     APYLSEAARQRIEQRRPVGAPVQGVVVGALRVVDLSLPQAGIPDARVAVVLEIESNMT
                     LGMAGAEQTQYARERWRLVRSAAARTKPPQDVRTFHCPNCGAPFEASDRPGGDRCSYC
                     GEVVSGGRFDWSVESIDLLSLEERPPALTTTVQEVGTNWPTVFHADLPARRAELLRDD
                     PAATDEALVARLQLIYNELNAAWTRLDLGGARPYVSDNLFDYLLYWTSAYQKQGLRNV
                     LENMRLMESTPVKVVRDRYYDALTFRIWGAGRDYTVRQGTGDVVSGNPRADRFYSEYW
                     TLIRGAGVKGAPRTERSCPNCGAPLDINMAGECEHCGAKITTGRFDWVLSKIEQDDSY
                     TG"
     gene            complement(4362..4985)
                     /locus_tag="VEL74_21230"
     CDS             complement(4362..4985)
                     /locus_tag="VEL74_21230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65119.1"
                     /translation="MARPFLNDEAKRAFTGAVEAVEAGSSAELVVAVRDHSGSYLHAA
                     LLGGVLVAVATLVVLLFSPWPFDLVWFVVDPLLLGLLAGFLTSRSPVLCRLLTRRAER
                     RRRVEIAARSLFVEKRVHGTTGRTGLLLYISILEREAELVVDLGVETLAGTEAWRRAV
                     AEIQEAVRRGEDGIAVAGKVQGLAALLSPVLARQAGDVDELANEVCE"
     gene            complement(5052..5804)
                     /locus_tag="VEL74_21235"
     CDS             complement(5052..5804)
                     /locus_tag="VEL74_21235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010373505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OBAP family protein"
                     /protein_id="HYG65120.1"
                     /translation="MTAPALFLLLACGGENREPGAEPAGAPTSARTDTLKAGSKVLQD
                     TAPVQKLDVYIAGFHPMKDDPSHQMEAHHYCRQVNEDFAQCALFDGNTDAANLNGIEY
                     IISERVFESLPADERKYWHPHNYEILSGQLVAPGLPDVAEKELMRSKMNSYGKTWHVW
                     MSGTPEMPGDKLPLGEAHLAWSFNRDGEARPDMVQNRDRRMGIDPVEERRQRADLATL
                     AKPQEGVGAIAGQLPGKGAPPGVVAKGSPGAQ"
     gene            5941..6339
                     /locus_tag="VEL74_21240"
     CDS             5941..6339
                     /locus_tag="VEL74_21240"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HYG65121.1"
                     /translation="MTSRRNKSTSMAIPLSAREVRRIVLSFPGIEEGTTYGTPSFRVK
                     GRFLARIDEKEEALILKVDFDRRDALLEEDPETFYITDHYVGYPMVLIRLATVQPRTL
                     RGLIEDEWRRLSSHRTVAAYDKHMSEIRRR"
BASE COUNT         1030 a         2235 c         2208 g          904 t
ORIGIN      
        1 gaccggaccg tcatcgccat ccccctcctc gacgagttcc gggcggagag ggacgcggcg
       61 gaggccgccc gcaagaagcg gcgccgggct cccgaggcca ggctctacga cgtcatcatc
      121 gacctcaacc tggactaccc cggcggccgc gaggaggcca agaagaccgt ccgggactgg
      181 atccagaagg cgatcgacgg cgtgggcacc cgcccaggcc agggcgtcca ccaggtgaag
      241 agcgagatca ccaatcaata tctgttcgcc cggctcgagc ggcgggtcgt cgaggagctg
      301 gtccggatca accatggcga aggccagccg gagaaggccc ccaggcgcgc cctgttccgc
      361 gtctggccgg acttcaaggt cggcccgctg ctcaccaggt cgctggctac ggtcaaggcc
      421 gacgcggcgc gggtctcctt ctcggcccag ggcaaggaca tcctctgggc ggtcctcgac
      481 tcggggatcc agaaggagca cccgcacttc cagaagcacg gcaacctcga cctgcccgcc
      541 cccttgaagc accgcgactt caccgcgctc gacgagagct ccgagcagcc gctggtcgac
      601 cgcttcggtc acggcacgca cgtcgccggc atcgtggcgg gcgagctcac cggccaggtg
      661 cgcacggtca cccgccaccg ggacgagcag ggggaggtct cctaccagac ctccgagctc
      721 gacgccatct ccggcgtcgc cccgctgtgc aagctgctca gcctcaaggt actggacgac
      781 gacggcaacg gcctggcgag caacctcatc gcggccatcg ccctgatcca ggaggcgaac
      841 ggccacggcc ggcggatcgt cgtccacggg gtcaacatga gcgtcggcta cgatttcgag
      901 ccggagtggt tcgcctgcgg ccagagcccg ctgtgcgtcg aggtggaccg cctggtccgc
      961 tccggcgtgg cagtggtggt ggcggcgggc aacaccggct acggcttcgc ccagagcgcg
     1021 ttccgcggca ccgtggccgc cggcatgaac ctgacgatca atgatccggg caacgccgag
     1081 ctggcgatca ccgtgggctc gacccaccgc gattcgcctc acatttatgg cgtctcgtat
     1141 ttctcttcga aaggccccac cggcgacggg cgcgccaagc ccgacctggt ggctcccggc
     1201 gagaagatcg tctcctgcgc cgccggcaag ccgcgcgagg agctgcggga gaaggccgcc
     1261 gactgcgatt atcgggagga gagcggcacc agcatggcgg ccccccacgt ctcgggcgcc
     1321 atcgccgcct tcctttccat ccgccgcgag ttcatcggcc agccggagaa ggtcaaggcg
     1381 atcttcatgg cgtcggccac cgacttgaac cgcgaaaggt atttccaggg gcgcggcctc
     1441 gtcgacctga tgcgcgcgat ccaatccgtc tgacacgccg gaggaaacca tggcagccat
     1501 ctcgggcttt ccctacttcg aaatcgagtt cgacaagaag ggcgcggcgc acgatccagc
     1561 ccagcggcag gcgctgctgg acttcgtcgc caagggcaag gatcaggtca ccgacctgct
     1621 ggtcctctcc cacggctgga acaacgacat ggcggacgcg cgggacctct accggaggct
     1681 cctcgcccgg ctgcgcgacg gaatcgacgg cggcaaggtg gccggggtga aggaaaggaa
     1741 attcgccatc tgcgcggtcc tctggccgtc gaagaaattc accgacaagg agctgatccc
     1801 gagcggcgcc gcgagcgccg gcggcgtcgt ctccacccag gcgctccagg aggagatcga
     1861 tcgcgtcaag gacgccttcg acagccccac ggccgccgcc gatctcgaca gggcgcgggc
     1921 gctggtgtcg aagctggagg acagcaaggc ggcccgcgac gagttcgcgc agatcctgcg
     1981 ctccgtcctg tccgcgggga ccggcaactc gaacgacgag gagaccccct ccgctttctt
     2041 cacattgccc ggcaacgaga tcatccagcg gctgagcaaa ccgctccccc tggccccggg
     2101 ggaacctgga ggcggagcgg ccggcggtgt gagcggtggc acgggcggcc ccgcaggtgg
     2161 tgcggcggcc ctcggaatct tttccggcat caagggcggg gcgatgaagc tgctcaacct
     2221 gaccacctat tatcagatga aggagcgggc cgggctggtg ggtagcaaag gggtgaatcc
     2281 gatcctgcgc gaggtggaga cgaagcggcc ggacttgaag ctgcatctga tcggtcacag
     2341 cttcggcggc cggctggtga ccgcggccgt gctcggcccg gatggcgcca cgccgatcaa
     2401 gccggaaacg ctgaccctgc tccaggccgc cttctcccac tacggctttt cgcaagggta
     2461 cgagccgggc aaggacgggt tcttccgcaa ggtggtgagc gagaagcggg tcgccgggcc
     2521 ggtgctcgcc acctgcaccc acaacgacaa ggcggtgggc aaggcctatc cgatcgcgtc
     2581 gcagatcgcc ggccaggtcg ccgccgcgct gggcgacaag aacagccgct ggggcggcat
     2641 cggcgccaac ggcgcgcaga agacgcccga gtccgtggat ggcaagctcc tgccggcggg
     2701 cggcgcctac tcgttcaccg ccggcaaggt tcacaacctc aacgccgaca gcttcatcaa
     2761 ggatcactcg gacatcgcca gggacgaggt ggcccacgcc gtgctgacgg cggtcgcggc
     2821 gacgtagccg ggctaccccg tgtaggagtc gtcctgctcg atcttggaga gcacccagtc
     2881 gaagcggccg gtggtgatct tggcgccgca gtgctcgcat tcgccggcca tgttgatgtc
     2941 gagcggagcg ccgcagttgg ggcaggagcg ctcggtgcgg ggcgcgccct tgaccccggc
     3001 gccgcggatc agcgtccagt actcggagta gaagcggtcc gcccgcgggt tgccggacac
     3061 cacgtccccc gtcccctggc gcacggtgta gtcgcggccc gccccccaga tccggaaggt
     3121 cagagcgtcg tagtagcggt cgcggaccac cttgaccggc gtcgactcca tcagccgcat
     3181 gttctccagg acgttgcgga ggccctgctt ctggtaggcg ctggtccagt agaggaggta
     3241 atcgaacagg ttgtccgaca cataggggcg tgcgccgccg aggtcgagac gggtccaggc
     3301 ggcgttgagc tcgttgtaga tgagctgcag ccgcgccacc agcgcttcgt cggtggccgc
     3361 ggggtcgtcg cgcagcagct ccgcccggcg cgccggcagg tcggcatgga agaccgtcgg
     3421 ccagttggtg cccacctcct gcaccgtcgt ggtcagcgcc ggcggccgct cctcgaggct
     3481 cagcaggtcg atcgactcga cgctccagtc gaaacggccg ccggagacca cctcgccgca
     3541 gtaggagcag cggtcgcccc ccggccggtc cgacgcctcg aagggggcgc cgcagttggg
     3601 gcagtggaag gtgcgcacgt cctgcggcgg cttggtgcgc gccgcggcgg accgcaccag
     3661 ccgccagcgc tcccgggcgt actgggtctg ctccgccccg gccatgccca gggtcatgtt
     3721 ggactcgatc tccagcacca cggcgacccg cgcgtccgga atcccagcct ggggcaacga
     3781 caggtccacc acccgcaggg cgccgacgac cacgccctgg accggcgcgc cgaccgggcg
     3841 gcgctgctcg atccgctgcc gggccgcctc gctcaggtag ggggccaagg cgtcgaggtc
     3901 ccgccggctg gcgtgggcct ggtaggcgcg ggcatagagc gcgtaggcga agtcctcgaa
     3961 gaggacgacg gagaagtccg ggtccagctt gcggatcgcc tccaggtcca gcgaccgcgg
     4021 acgggccgcc ggccccgccg gcgggtgggc gtgcgacggc gcggcatccc aattctgggg
     4081 ccccgccttg gccttcttct tccgcttgaa gtgcaggtag agaaggacgg cgatggtgat
     4141 cgggatgccg atgtgcgggt aggcgataca gaactcgatc cacaaccgca gcaacagcca
     4201 gacgaacccc gagtcgccgt cccccccgcc gcctccgccg ctgtcgccac cgccggaata
     4261 gccctcgccg ccacccggac gggcgacggc gagccccggc accaggaccg ccagcgccgc
     4321 gaggatcagc ccgaacgcca gcagcccgcg gcgcggccgg ctcactcgca cacctcgttg
     4381 gccagctcgt cgacgtcccc cgcctgccgc gccaggacgg gcgacaggag cgccgcgaga
     4441 ccctggacct tgcccgccac ggcgatgccg tcctcgccgc gccgcaccgc ctcctggatc
     4501 tcggccaccg cgcgccgcca ggcttcggtg ccggcgagcg tctcgacgcc gaggtccacc
     4561 accagctccg cctcccgctc gaggatcgag atgtagagga ggagcccggt ccgccccgtg
     4621 gtgccgtgga cccgcttctc gacgaacagc gaccgcgccg cgatctcgac ccgccgccgc
     4681 cgctcggcac ggcgggtgag cagccggcag aggaccggcg agcgcgaggt caggaagccg
     4741 gcgaggagcc ccaggaggag ggggtccacc acgaaccaga ccaggtcgaa cggccagggg
     4801 gagaagagga ggaccaccaa cgtggccacg gccacgagga cgccgccgag gagggcggcg
     4861 tgcaggtacg acccggaatg gtcgcggacc gccactacca gctcggcgct gctcccggcc
     4921 tccaccgcct cgacggcacc ggtgaaggcc cgcttcgcct cgtcattgag aaagggtctg
     4981 gccatggtcg cgagggggtg gcaaaggcgc cgacagtgta tcccaagcga gcaggccagc
     5041 cctccccgat cctactgagc ccccggcgag cccttggcca ccactcccgg cggtgccccc
     5101 ttccccggga gctggccggc gatggcgccg acgccctcct gcggcttggc gagggtggcg
     5161 aggtccgccc gctggcggcg ctcctccacg gggtcgatac ccatgcggcg gtcccggttc
     5221 tgcaccatgt cgggccgcgc ctcgccgtcg cggttgaacg accaggccag gtgcgcctcg
     5281 cccaggggca gcttgtcccc cggcatctcc ggcgtgccgc tcatccagac gtgccaggtc
     5341 ttgccgtagc tgttcatctt ggagcgcatc agctccttct cggcgacgtc cggcagcccg
     5401 ggggccacga gctggccgga gaggatctcg tagttgtggg ggtgccagta cttcctctcg
     5461 tccgccggca gcgactcgaa gacccgctcc gagatgatgt attcgatgcc gttcaggttg
     5521 gcggcgtcgg tgttgccgtc gaacagggcg cactgggcga agtcctcgtt gacctgtcgg
     5581 cagtagtggt gggcctccat ctggtgggac ggatcgtcct tcatcggatg gaaaccggcg
     5641 atgtagacgt ccagcttctg gaccggcgcg gtgtcctgga gcaccttgga gccggccttg
     5701 agggtatccg tcctggcgct ggtgggggcg ccggcgggct cggccccggg ctcccggttt
     5761 tccccgccgc aagcgaggag gaggaagaga gccggagcgg tcaggagggt aagcaggggg
     5821 tgtttcggca cgaagggcct cctttccaga actcctctcc atctgcaagg cgtattccgg
     5881 agtgtctcgg catacaattg cgcgcgccgg caccctggca gccgtgccga ggagatccga
     5941 atgacgagtc gacggaacaa aagcacgtcc atggcgatcc cgctgtctgc acgtgaggtg
     6001 cgccggatcg tgctctcttt tcccggaatc gaggaaggaa cgacctacgg tacgccgtcg
     6061 ttccgggtga aggggaggtt cctggcccgg atcgacgaga aggaggaggc gctcatcctc
     6121 aaggtggact tcgaccgccg cgacgccctg ctggaggagg atccggagac tttctacatc
     6181 acggaccact acgtcggcta tccgatggtc ctcatccggc tcgccaccgt ccagccccgg
     6241 accctgcgcg ggctgatcga ggacgagtgg cggcggctct cctcccaccg cacggtcgcc
     6301 gcgtacgaca agcacatgag cgagatccgc cgccgctgac gtccttccgg tctccgcgca
     6361 tacaatcccc ccgcgct
//
LOCUS       DASPJI010000756        12098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_48689, whole genome shotgun sequence.
ACCESSION   DASPJI010000756 DASPJI010000000
VERSION     DASPJI010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12098)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12098
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48689"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VEL74_21245"
     CDS             <1..440
                     /locus_tag="VEL74_21245"
                     /inference="COORDINATES: protein motif:HMM:NF013156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HYG65122.1"
                     /translation="GITFTAESGGGSNSISLASGSGTDLNNLVLEVRANSVQDLYGVA
                     FDLQYPTTVLRYDSATAGFLNEGAAQTSLNLVEASPGTLVVGYTRLGPVGGATGSGVL
                     LTLRFSAVAAGSGNFAFAQSSAFNPRGDSSAVTWSAGSVQVVR"
     gene            460..1875
                     /gene="cysS"
                     /locus_tag="VEL74_21250"
     CDS             460..1875
                     /gene="cysS"
                     /locus_tag="VEL74_21250"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HYG65123.1"
                     /translation="MPELALYNTLGRKLSPFAPLVPREARMYVCGPTVYNEVHIGNLR
                     TFLVGDLLRRTLAYLGYRVTQVMNLTDVDDKTIRGAAAAGVSLSEYTAPFVQTFLRDL
                     DTLHLERPEHFPKATEHVPEMIAMIQRLVDEGYAYESEGSVFFAIAKDPDYGRLSGFD
                     LSQARQGERVASDEYGKEDVRDFVLWKAVKPGEPAWDSPWGPGRPGWHIECSAMSLKY
                     LGETFDLHCGGVDLIFPHHENEIAQSESATGHPFVRYWVHSEHLIVDGQKMSKSLGNQ
                     YTLPELISRGYAPRAIRYLFLSVHYRQKLNFTFDSLEGAAGALRRVDEMRFRLSHSVE
                     KGDPDPGTAESAERLRRDFAAAVADDLNFAGALAAVFNFVREINVAIEAGRIGTGDRE
                     RILAALADVDRVLGVLDPAEWPAQADGENDDAEIDRLVQERNDARKRRDFKESDRLRD
                     KLAARGIVLEDTPQGTRWKRR"
     gene            1872..2270
                     /locus_tag="VEL74_21255"
     CDS             1872..2270
                     /locus_tag="VEL74_21255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HYG65124.1"
                     /translation="MSGAPGGGFRQQPHNRARGRVGEDDAVRWLESQGYRVVERNVTN
                     HGGEIDLVAREGETLCFVEVKARASRSYGPAIAAVDGRKQRRISRAASLYIAIRGLHR
                     LPARFDVLGLDWTGSGWRYTLVRNAFPYTP"
     gene            2372..2446
                     /locus_tag="VEL74_21260"
     tRNA            2372..2446
                     /locus_tag="VEL74_21260"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2404..2406,aa:Gly,seq:gcc)
     gene            2511..2585
                     /locus_tag="VEL74_21265"
     tRNA            2511..2585
                     /locus_tag="VEL74_21265"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2543..2545,aa:Cys,seq:gca)
     gene            complement(2601..5921)
                     /locus_tag="VEL74_21270"
     CDS             complement(2601..5921)
                     /locus_tag="VEL74_21270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HYG65125.1"
                     /translation="MRSIKRTLLPTLLTLTLLALPAAGADKAAGKKDKEAGKSPRQTG
                     EVWSGLELRPIGPALTSGRISDIVVDPTRPATWYVAVASGGVWKTTNAGTTWTPIFDN
                     EGSYSIGCLALDPKNPLVVWVGTGESNGQRSVSYGDGVYRSEDGGKSWTRMGLEKSEH
                     VGRILVDPRDSSTVFVAAQGPLWSPGGDRGLYKTTDGGKTWKAVLTVSENTGVTDVVF
                     DPADPDTLYAAAWQRRRHVWTMINGGPESAIYRSTDGGATWAKVTTGLPREDIGRIGL
                     AVAPTRPGTVYAVVEAANGASGFFRSADRGLTWEKRGSYTPGGPMYYQQITVDPADPE
                     RIYSMDVFLMVSDDGGKSFRRLGETSKHVDNHVIWVDPRNTEHYLVGGDGGLYESFDA
                     GASWDWSANLPVTQFYRVAVDDSSPIYRVYGGTQDSFSLGGPSRTLTTHGVTNREWEV
                     FGTGDGFQPRVDPTDPNIVYSMAQHGYLRRFDRRTGEDVTIQPQTGKGEPPLRWNWDS
                     PLLVSPHSPTRLYFGANRVFRTDDRGQSWRAVSEDLTRQIDRNKLPVMGKIWSVNAVA
                     RHASTSFYGNIVALDESPRVEGLLYVGTDDGLIQVSEDAGRSWRRVERFPGVPENAYV
                     SRLNASFHDDGTVYAAFDHHKMGDFKPYVLRSTDRGRTWTSIAGDLPERGTVYALVED
                     PDAPGLLFAGTEFGLFFTRDGGKRWVRLRGGLPTIQVRDLAIQRREKDLVVGTFGRGI
                     YILDDYTPLRHGTPEALEREAALFPVRTAWLYSPSSPLGYRGKSFQGDSLYTAPNPPF
                     GAVFTYHLKEEIQTRRKARQAKEKETGKEGGTVAIPTLDEMRAEAAEVAPALVFTITD
                     ESGQVVRRLTAPTTAGVQRVAWDLRWPPADPADLTPPTVDPADPYIPIPAGPLVAPGT
                     YRVALAKSVDGRETPLGEPQAFEVRPPVTGPLSTGDRAALQEFVRKVASLQRAAQGAE
                     RAVDEMEDRIEHLRVALRDTPAPVAGLAEQLLGLSGRLRAAKLELSGDEVLRRLNEPV
                     PPSILDRIGAVVDSHWSTSSAATATSQEAYRIAAEDFESVLADLGKVRTDLESLEARV
                     EAAGAPWTPGRLPEWKQE"
     gene            6136..6999
                     /locus_tag="VEL74_21275"
     CDS             6136..6999
                     /locus_tag="VEL74_21275"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG65126.1"
                     /translation="MRISRWHCLHDGEVFAEERCHTTHVLTVIRAGTFALRHGRRSRL
                     LDPSCAVLHAPGIGYSATHPLGCGDHGWHVGLEKGALRPALPDGEEELAMPAETRVIP
                     LPSRAFAALRLALAPERGKGSPEPMAVEEAVLRLAGAMAAAVPARRQRPRRVSTEELH
                     ERCFERAREVLFTRRREAIHLDELARAVHSSPYHLSRLFKRAAGVPIHRYLSRLRLLD
                     GLEEIATREVALTELAYELGFSSHSHFTAAFHREFGMTPSRFRDLATGRSVSRASHAL
                     GEACRVSHLVG"
     gene            7049..8230
                     /locus_tag="VEL74_21280"
     CDS             7049..8230
                     /locus_tag="VEL74_21280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG65127.1"
                     /translation="MNLLVLATKAPWPPIDGGRLLLLHTLEGLAAAGCRPVLVAPVNP
                     AQFDLETVAGELSTWCEPRLVPASPVPAAAALARSWRAPLAITRHSLAPVRREVEELL
                     RTSRFDLVHAEQLQALPQAEPAFRRGIPVVLRAQNVESDLWAALARARGGFVGRALAF
                     EARRLARWEGEAVRRAAATFALTSEDAARLHALAGNGGSSRVRVVRAPFPGLPPGKGH
                     LPGEPSLVLLGSRGWLPNEDAVAWFLEEIWPAVRAELPGAVLHLFGAGAPPQASVVSH
                     PSPADSAEAFAPGSILVVPLRIASGVRMKVLEAWARGVPVVGTPTALAGLEVEDSREA
                     LVAADAPGFTRALARLHREGGLAAALVDEGKWALSGRHEPAGVATELLAAYAETTRQP
                     S"
     gene            complement(8312..9085)
                     /locus_tag="VEL74_21285"
     CDS             complement(8312..9085)
                     /locus_tag="VEL74_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002601632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG65128.1"
                     /translation="MSEPRIDLEGVSLCYRLAKQRLPSIKEYMIHFMKGALSYEKLWA
                     LRDITFKVEPGEVLGIVGRNGAGKSTLLKVISRVLKPTGKVVVRGTVSPILELGTGFD
                     HELTGFENIYLNALLLGRSRKEIDVKLEEIIQFSGLGDFIRAPIRNYSSGMMARLGFS
                     IATAWVPDILILDEVLAVGDAAFTEKCEHRMQQFHDAGTTVLMVSHSAESVRKNCTRC
                     IWLDAGNLRADGPAKEVLEMYSHAGTDPDQRAIGQLHLG"
     gene            complement(9099..9869)
                     /locus_tag="VEL74_21290"
     CDS             complement(9099..9869)
                     /locus_tag="VEL74_21290"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG65129.1"
                     /translation="MKEILSYRDLILTLVARDLKVRYRRSALGFLWTMLQPLLMMLVF
                     YAVFSALFRFSIPNYEVYALAGVLFWNFFSQSIVSSMNSLRGNAQLLKKLPVPKIVFP
                     LATVLSGVVNLLLALVPLLLIMLITGGLASLKPALLFLPVSILLAALFTLGAGLLLAP
                     LAVFFSDVLELIGVLLSVVMYLTPIFYPKEILPESLRWVVRFNPVRSILEVFRDPIYQ
                     GEVPPLTHLSVCIVIAILALVIGAVAFRRSSDRIPFYV"
     gene            10142..11389
                     /locus_tag="VEL74_21295"
     CDS             10142..11389
                     /locus_tag="VEL74_21295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65130.1"
                     /translation="MRSLWKRAALVAGVLVVAVAPWSEAQTMDGAAQEASVKARAEAV
                     TTVRDMLLKRSVRKVDPKIAEADARRIVSKLSEEQVGAVLDGQDLVSIFAADKARMSA
                     AVTAPSDASVTNVSAKALGDPESELLFVPVEPCRIIDTRVAGGPVAANTNRDFRVTGT
                     TGFEAQGGNAGGCGIPIGSVDPLAPAVVINFIAVGPSGPGNLRAWEFGQPIPRASVIN
                     YANVTGLNIANGVIVPVAGVSTAASDMHIRADAAGTHVVADVTGYFTRFPTEEFQGGL
                     QTKLVTTANSVLVDLGDGGCKELTTCTVTTTAPGQVMIEAWAQVVAGHTSGTEDRFVM
                     QLETAASVSCPEDDSVNASDYEIPAALGTNADVDFTLSHARVFAQAAGQTRTYRLSGR
                     MVNGANVQDQVENSRVLCTFIPD"
     gene            11410..>12098
                     /locus_tag="VEL74_21300"
     CDS             11410..>12098
                     /locus_tag="VEL74_21300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65131.1"
                     /translation="MKTLWQTSMTAACAALFAALLAGPAAAQLQPAAEDGPREKARAL
                     IAETLEKRVVRPVAPERIEAEAERIVASLPEEQVQTLVETRDAAPVLEERTRAAKASQ
                     PLAVISGKALGDSQSDLVFVPVAPCRIIDTRQVSAGPLAANETRHFLVAGTNAFLAQG
                     GTGGGCGVPDGVSEPAAPAVVINFIAVGPSGPGNLRAWEYGQPVPRASVINYANVPGL
                     NIANGVVVPIEG"
BASE COUNT         1772 a         4205 c         4227 g         1894 t
ORIGIN      
        1 cggggatcac cttcaccgca gagagcggcg gcggctcgaa cagcatctcc ctggccagcg
       61 gctcgggtac cgacctcaac aacctggtgc tcgaggtgcg ggccaacagc gtgcaggacc
      121 tctacggcgt ggcgttcgac ctgcagtacc cgacgacggt gctgcgttac gacagcgcca
      181 ccgccggttt cctcaatgaa ggcgcggcgc agacctcgct gaacctggtg gaggcgtccc
      241 cggggaccct ggtggtgggc tacacccgcc tgggaccggt gggcggcgcc acaggctccg
      301 gcgtgctgct gaccctgcgt ttctccgccg tcgcagccgg cagcggcaac ttcgccttcg
      361 cccagagctc ggccttcaac ccgcgcggcg attccagcgc cgtcacctgg tcggccggca
      421 gcgtccaggt cgtccgctga gccccagcct cgatccgaca tgcccgaact ggccctctac
      481 aacacccttg ggcgcaagct ctccccattc gcccccctgg tcccacgcga ggccaggatg
      541 tacgtctgcg gccccacggt ctacaacgag gtgcacatcg gcaacctccg cacctttctg
      601 gtcggggacc tgctgcgccg gaccctcgcc tacctgggct accgcgtgac gcaggtgatg
      661 aacctgacgg acgtggacga caagacgatc cggggggccg ccgcggccgg cgtctcgctc
      721 tccgagtaca ccgcgccgtt cgtccagacc ttcctgcgcg acctggacac cctccacctg
      781 gagcgccccg agcacttccc caaggccacc gagcacgtgc cggagatgat cgccatgatc
      841 cagcgcctgg tggacgaggg ctacgcctac gagagcgagg gctcggtctt cttcgccatc
      901 gccaaggacc ccgactacgg gcggctctcg ggcttcgacc tgagccaggc gcgccagggg
      961 gagcgggtgg cgtcggacga gtacggcaag gaggacgtgc gggacttcgt gctgtggaag
     1021 gcggtcaagc ccggcgagcc ggcgtgggac tccccctggg ggcccggccg gcccggctgg
     1081 cacatcgagt gctccgccat gagcctcaag tacctgggcg agaccttcga cctccactgt
     1141 ggcggcgtcg acctgatctt cccgcaccac gagaacgaga tcgcgcaaag cgagtcggcc
     1201 accggccacc ccttcgtgcg ctactgggtc cactcggagc acctgatcgt cgacggccag
     1261 aagatgtcga agtcgctcgg caatcagtac acgctcccgg agctgatctc ccggggctac
     1321 gcgccgcggg cgatccgcta ccttttcctg tcggtccact accggcagaa gctgaacttc
     1381 accttcgact ccctcgaggg ggcggcgggg gcgctgcggc gggtcgacga gatgcgcttc
     1441 cggctttccc actcggtgga gaagggggat ccggacccgg ggaccgccga gtccgccgag
     1501 cgcctgcgcc gcgacttcgc cgccgcggtc gccgacgacc tcaacttcgc cggcgcgctg
     1561 gccgcggtct tcaacttcgt ccgcgagatc aacgtcgcca tcgaagccgg ccggatcggc
     1621 acgggcgacc gagagcgaat cctcgcggcg ctggcggacg tggaccgggt gctgggggtg
     1681 ctcgatccgg cggagtggcc ggcgcaggct gacggcgaga acgacgacgc ggagatcgac
     1741 cgcttggtgc aggagcgcaa cgacgcccgc aagcgccgcg acttcaagga gtccgaccgc
     1801 ctccgcgaca agctggccgc ccgcggcatc gtgctggagg acaccccgca gggcacgcgc
     1861 tggaagcggc ggtgagcgga gcgccggggg gaggcttccg gcagcagccc cacaaccgcg
     1921 cccgcggccg ggtgggggag gacgacgcgg tccgctggct tgagtcccag ggctaccggg
     1981 tggtcgagcg caacgtcacc aaccacggcg gcgagatcga cttggtggcg cgggagggtg
     2041 agaccctctg cttcgtcgag gtcaaggccc gggccagccg gtcctacggc ccggccatcg
     2101 ccgccgtcga cggccgcaag cagcgccgga tctcccgcgc cgcctccctc tacatcgcca
     2161 tccggggcct ccaccgcctc cccgcccgct tcgacgtcct cggcctggac tggaccggct
     2221 cggggtggcg ctacaccctg gtccgcaacg ccttccccta caccccctga ggccctcgtt
     2281 gccgctccgc ggcaacgggg gaatacgaga tcccagaaag ggggttgaca ggccacagga
     2341 gcatggctat tctttgccgg ctcgtgagcg agcgggaata gctcagtggt agagcacaac
     2401 cttgccaagg ttggggtcgc gggttcaaat cccgtttccc gctccaaaat tcaagctaca
     2461 atcgaaaggg ccgcttccgc ggcccttccg ttcaagagcg gttcattcaa ggcgacgtag
     2521 ccaagtggta aggcgagggt ctgcaaaacc cttattcatc ggttcgaatc cgatcgtcgc
     2581 ctccaacatc ttcccccctc tcactcctgt ttccactccg gcagccggcc cggcgtccag
     2641 ggcgctcccg cggcttccac ccgcgcctcg aggctctcca ggtctgtcct gaccttcccc
     2701 agatccgcca gcaccgactc gaaatcctcc gcggcgatgc ggtaggcctc ctggctggtc
     2761 gccgtcgcgg ccgagctggt cgaccagtgg ctgtccacga cggcgccgat ccggtcgagg
     2821 atcgacggag ggacgggctc gttgaggcgg cgcagcacct cgtcacccga cagctccagc
     2881 ttggcggccc gcaggcggcc ggagaggccc agcagctgct cggcgagccc cgctaccggg
     2941 gccggcgtgt cgcgtagcgc cacccgcagg tgctcgatcc ggtcctccat ctcatcgaca
     3001 gcccgctccg ccccctgggc ggcgcgctgc aggctggcga ccttgcgcac gaactcctgg
     3061 agcgcggcgc ggtctccggt ggacagggga ccagtcaccg gtggccggac ctcgaaggcc
     3121 tggggctcgc ccaggggcgt ctcccggccg tccacgctct tcgccagagc cacgcggtag
     3181 gtccccggcg ccaccagcgg gccggcgggg atggggatat aggggtccgc cgggtccacg
     3241 gtgggcggcg tcaggtcggc ggggtcggcg ggcggccagc gcaggtccca ggcgacgcgt
     3301 tggacgcccg cggtggtggg cgcggtcagg cggcggacca cctggccgga ctcgtcggtg
     3361 atggtgaaca ccagcgccgg cgccacctcg gcggcctcgg cccgcatctc gtcgagcgtc
     3421 gggatggcca ccgtgccccc ctccttcccg gtctccttct ccttcgcctg ccgggccttg
     3481 cggcgggtct ggatctcctc cttcaggtga taggtgaaga cggcgccgaa gggcgggttc
     3541 ggcgcggtgt agagggagtc cccctggaag gacttgccgc ggtagcccag gggggacgac
     3601 gggctgtaga gccaggccgt ccgcaccggg aagagcgccg cctcccgctc cagcgcctcg
     3661 ggcgtgccgt ggcgcagggg cgtgtagtcg tccaggatgt agatgccgcg tccgaaggtc
     3721 cccaccacca ggtccttctc ccgtcgctgg atcgccaggt cccgcacctg gatggtgggc
     3781 aggccgccgc gcaggcgcac ccaccgcttc cctccatccc gggtgaagaa gaggccgaac
     3841 tccgtgccgg cgaagagaag acccggagcg tcggggtcct cgaccagggc gtagaccgtc
     3901 ccccgctcgg gcaggtcgcc ggcgatggag gtccaggtgc gcccgcggtc ggtgctgcgc
     3961 agcacgtagg gcttgaagtc gcccatcttg tgatggtcga aggcggcgta gaccgtgccg
     4021 tcgtcgtgga aggaggcgtt gaggcggctg acataggcgt tctcgggcac gcccgggaag
     4081 cgctcgacgc ggcgccagct ccggccggcg tcctccgaca cctggatcag gccgtcgtcg
     4141 gtgcccacgt agaggagccc ctccacgcgg ggggactcgt ccagcgccac gatgttgccg
     4201 tagaacgagg tcgacgcgtg gcgtgccacg gcgttgaccg accagatctt gcccatgacc
     4261 ggcagcttgt tgcggtcgat ctggcgggtg aggtcctcgg agaccgcccg ccagctctgc
     4321 ccccggtcat cggtgcggaa cacgcggttg gcgccgaagt agaggcgtgt cgggctgtgc
     4381 gggctcacca gcagcggcga gtcccagttc cagcgcagcg gcggctcccc cttgccggtc
     4441 tgcggctgga tggtcacgtc ctcgccggtg cgccggtcga agcggcggag gtagccgtgc
     4501 tgcgccatcg agtagacgat gttcgggtcg gtggggtcga cccgcggctg gaagccgtcg
     4561 ccggtgccga agacctccca ctcgcggttg gtgacgccgt gggtggtaag ggtgcgcgag
     4621 gggccgccga gggagaagct gtcctgggta ccgccgtaga cccggtagat gggggaggag
     4681 tcgtcgacgg cgaccctgta gaactgggtg accggcaggt tggccgacca gtcccagctc
     4741 gcgccggcgt cgaagctctc gtagaggccg ccgtcgcccc ccaccaggta gtgctcggtg
     4801 ttgcgcggat cgacccagat cacgtggttg tcgacgtgct tcgaggtctc gcccagacgc
     4861 ctgaagctct tgccgccgtc gtcgctcacc atgaggaaga cgtccatgga gtagatccgc
     4921 tcgggatcgg ccgggtcgac ggtgatctgc tggtagtaca tggggccgcc cggggtgtag
     4981 ctgccgcgct tctcccaggt gaggccgcgg tcggccgacc ggaagaagcc gctggcgccg
     5041 ttggccgcct cgacgacggc gtagacggtg cccggccggg tcggggccac ggcgaggccg
     5101 atccgcccga tgtcctcgcg cggcaggccg gtggtcacct tggcccaggt ggccccgccg
     5161 tcggtggaac ggtagatggc cgactccggg ccgccgttga tcatggtcca gacgtggcgc
     5221 cggcgctgcc aggcggcggc atagagggtg tcggggtcgg ccgggtcgaa gacgacgtcg
     5281 gtgacgccgg tgttctcgct gaccgtcagc acggccttcc aggtcttgcc gccgtcggtc
     5341 gtcttgtaga ggccgcggtc gccgccaggg gaccagaggg ggccctgggc cgccacgaag
     5401 acggtggagg agtcgcgggg atcgacgagg atgcggccga cgtgctcgga cttctcgagc
     5461 cccatccggg tccacgactt cccgccgtcc tcggacctgt agacgccgtc accgtaggag
     5521 acgctgcgct ggccgttgct ctcgccggtc cccacccata ccaccagggg gttcttcggg
     5581 tccagggcga ggcagccgat ggagtaggac ccctcgttgt cgaagatggg cgtccaggtg
     5641 gtgcccgcgt tggtggtctt ccagacgccg ccggacgcca cggccacgta ccaggtggca
     5701 gggcgggtgg ggtccacgac gatgtcggag atccggccgg acgtgagcgc cggtccgatg
     5761 ggccgcaact ccaggccgct ccagacctcg ccggtctggc gggggctctt cccggcctcc
     5821 ttatccttct tgcctgcggc cttgtcggcc cccgccgccg ggagggcgag cagggtcaga
     5881 gtcagcaggg tcggtagcag cgttcgcttg atcgatcgca cgtctttctt cctcctcgtg
     5941 ggctgtcggc tttatagacc atctgaggaa cggccgcgct gtcgaaaagt tgctcgtttt
     6001 cagccgcagg gccgatcggg ccgaaccttg ggagtgccca gccgtagaag ggccatggac
     6061 aatccgcatc gaggccccgg gtgggagagg atcgtggaat atgccaacct ctctccgatc
     6121 ttcgatagcc gcttggtgag gatctctcgc tggcactgcc tccacgacgg ggaggtcttc
     6181 gccgaggagc ggtgtcacac cacgcatgtg ctgacggtga tccgcgcagg gacgttcgcc
     6241 ctgcgtcacg gccggcggtc gagactgctg gacccgtcct gcgcggtcct gcacgccccg
     6301 ggtatcgggt attcggcgac gcacccgctc ggctgcggag atcacggttg gcacgtcggg
     6361 ctcgaaaagg gggccttgcg cccggccctc ccggatgggg aagaggagct tgccatgccg
     6421 gccgagaccc gggtcattcc cctgccgtcc cgcgctttcg cggcgttgcg tctggccctc
     6481 gcgccggagc gcgggaaggg ctcccccgag cccatggctg tcgaggaggc cgtcctccgg
     6541 ctggcagggg ccatggccgc ggcagtcccc gcgaggcggc agcgcccccg ccgggtttcg
     6601 accgaggagc tccacgagcg ctgcttcgag cgcgcccggg aggtgctctt cacccgccgc
     6661 cgcgaggcga tccacctcga cgagttggcg cgggcggtcc attcctcgcc gtaccacctc
     6721 agccgtctgt ttaagcgggc ggcgggggtg ccgatccacc gttatctgag ccgtctgcgc
     6781 ctgctcgacg gcctcgagga gatcgccacg cgggaggtgg ccctgaccga gctggcctac
     6841 gagctgggtt tttcgagcca cagccacttc accgccgcct tccaccgcga gttcggcatg
     6901 accccctcgc gcttccgcga cctcgccacc gggcggagcg tgtcccgcgc cagccacgct
     6961 ctcggtgagg cgtgccgggt gtcgcatctg gtggggtagg gggagggata cccagagatc
     7021 cggcatcggg accgatatac ttcggccctt gaacctcctc gtcctcgcca ccaaggcccc
     7081 gtggcctccc atcgacggtg gccggctcct gctgctccat accctggaag ggctcgcggc
     7141 ggccgggtgc cggccggtgc tggtggcgcc ggtcaacccg gcgcagttcg atcttgaaac
     7201 ggtggcgggg gagctttcga cgtggtgcga gcctcggctg gtgccggcct ccccagttcc
     7261 ggcggctgcc gcgctggctc gctcctggcg cgccccgctg gcgatcaccc gccattcgct
     7321 ggctccggtg cggcgggagg tggaggagct gttgcggacg tcgcgcttcg atctcgtcca
     7381 tgccgagcag ctccaggcgc ttccccaggc cgagccggcc ttccggcggg gcatccccgt
     7441 ggtgctgcgg gcccagaacg tggagagcga cctctgggcc gccctggccc gcgcccgtgg
     7501 cggattcgtg ggacgggccc tcgcgttcga agcccggcgg ctcgcccgat gggaagggga
     7561 ggcggtccgc cgtgccgcgg ccacgttcgc ccttacctcc gaggacgcgg cgcgcctgca
     7621 tgcgctggcc gggaatggag gctcctcccg cgtccgggtg gtacgggccc cctttcccgg
     7681 gctccctccc ggcaaggggc accttcccgg tgagccatcc ttggtccttc tgggcagccg
     7741 gggctggctt cccaatgagg acgcggtcgc ctggttcctg gaagagatct ggcccgccgt
     7801 ccgcgccgag ctgccgggcg ccgtcctcca tctcttcggc gccggggccc ctccacaggc
     7861 cagcgtcgtc tctcatccgt caccggcgga cagcgccgag gcgttcgctc ccggttccat
     7921 cctggtggtc cccctgcgga tcgcctccgg ggtccggatg aaggttctgg aggcctgggc
     7981 gcggggagtg ccggtcgtgg gaacccccac cgctctcgcc ggcctggagg tggaggatag
     8041 ccgcgaagcg ctcgtcgccg ccgacgcccc ggggttcact cgcgccctgg cccgtcttca
     8101 tcgcgagggt ggcctcgcgg cggcgctggt ggatgagggg aagtgggcgc tgagcggacg
     8161 gcacgagccg gcgggggtgg cgacggagct gctcgcggct tatgcggaga ccacgcgcca
     8221 gccatcgtga tcaaggatcg tgggcgacgg ctgccccggg agggatgaac ccgtctccgt
     8281 gggccgcggc gtcaggtagc tccggagggc cctaccccag gtgcaactgc ccgatcgccc
     8341 gctggtcggg gtcggtgccg gcgtgggagt acatctccag cacttccttg gcggggccgt
     8401 cggcgcgcag gttgccggcg tccagccaga tgcagcgggt gcagttcttt cgcaccgact
     8461 cggcgctgtg ggagaccatc agcaccgtcg ttccggcgtc gtggaactgc tgcatccggt
     8521 gctcgcactt ctcggtgaag gcggcgtcac ccaccgccag gacctcgtcg aggatcagga
     8581 tgtccggcac ccaggcggtg gcgatggaga agccgaggcg ggccatcatg ccgctcgagt
     8641 agttgcggat cggcgcccgg atgaagtcgc ccaggccgct gaactggatg atctcctcca
     8701 gcttcacgtc gatctccttg cgcgagcggc cgaggagcag ggcattcaga tagatgttct
     8761 cgaatcccgt cagctcgtgg tcgaagcccg tcccgagctc caggatgggg gagaccgtcc
     8821 cccgcaccac caccttgccg gtgggcttga gcacccgcga gatcaccttg agcagcgtgc
     8881 tcttgcccgc gccgttgcgg cccacgatcc ccagcacctc cccgggctcc accttgaacg
     8941 tgatgtcacg caaggcccag agcttctcgt aggagagcgc ccccttcatg aagtggatca
     9001 tgtactcctt aatggagggg aggcgctgct tggccagccg gtagcagagg gagacccctt
     9061 ccaggtcgat gcgcggctcg ctcatccggc tccgggcctc agacatagaa ggggatccgg
     9121 tcgctgctgc ggcggaaggc cacggcgccg atcaccaggg ccaggatcgc gatgacgatg
     9181 cagaccgaga ggtgggtgag gggcggcacc tccccctggt agatggggtc gcggaagacc
     9241 tccaggatcg aacgcaccgg gttgaaccgc accacccagc ggaggctctc gggcaggatc
     9301 tccttcggat agaagatcgg cgtcaggtac atcaccaccg acagcagcac cccgatcagc
     9361 tcgagcacgt cgctgaagaa gacggccagc ggcgccagca gcagccccgc ccccagggtg
     9421 aagagggcgg cgaggaggat cgacaccggc aggaagagca gcgccggttt cagcgatgcc
     9481 aaccccccgg tgatcagcat gatcagcagc agcggcacca gggcgagcag caggttcacc
     9541 acgccggaga gcaccgtcgc cagcgggaag acgatcttgg gcaccggcag cttcttgagg
     9601 agctgcgcgt tgccgcgcag gctgttcatc gacgacacga tgctctggct gaagaagttc
     9661 cagaacagga ccccggccag cgcgtagacc tcgtagttgg ggatgctgaa ccggaaaagc
     9721 gcgctgaaca cggcgtagaa gaccagcatc atcagcagcg gctgcagcat ggtccagagg
     9781 aagccgagcg ccgagcgccg gtagcgcacc ttgaggtccc tcgccaccag ggtgaggatg
     9841 aggtcgcggt agctgaggat ctctttcatg gtctgaagtt ccggcctgcg gaccggtttc
     9901 tacaccgagc cgggcccgag agcaagtttt acccgcattc tcccctgctg cccccctcgc
     9961 cagagtgccc tccaccccag ggaaaccggg ccgttcccgc gtggcctggt ctttgcacaa
    10021 agggcgggca gaggattctg tgcgagtttg atcctccttt tgtcttcctc tgtgatggtt
    10081 gccgcggaag tgtagtaggc cctcatcgac aacttggttt ttgtatgcga aaggagtatc
    10141 catgaggagt ttgtggaagc gagcagccct ggttgcgggt gtactcgtgg tcgccgttgc
    10201 cccctggagc gaggcgcaga caatggatgg cgcggctcag gaggcgagcg tcaaggcgcg
    10261 ggcggaagcc gttacgactg tccgtgacat gctgctcaag cgttcggtgc gcaaggtgga
    10321 tcccaagatc gccgaggcgg acgcgcggcg catcgtctcc aagctctccg aggagcaggt
    10381 cggggccgtg ttggacggcc aggacctggt ctcgatcttc gcggcggaca aggcacggat
    10441 gtcggcggcg gtgaccgccc cctcggacgc ctccgtgacc aatgtttcgg ccaaggcttt
    10501 gggcgatccc gagtcggagc ttctcttcgt cccggtggaa ccctgccgca tcatcgacac
    10561 ccgggtggcc ggcgggcctg tcgccgcgaa cacgaaccgt gacttccggg tcaccggtac
    10621 cacgggcttc gaggcccaag gcggcaacgc gggcggctgc ggcatcccga tcggctcggt
    10681 ggatcccttg gcgccggcgg tggtgatcaa cttcatcgcg gtcggcccgt ccggacccgg
    10741 caacctgcgc gcctgggagt tcggccagcc catcccccgg gccagcgtca tcaactacgc
    10801 caacgtcacc gggctcaaca tcgccaacgg cgtcatcgtc cccgtggccg gcgtctccac
    10861 ggcggccagt gacatgcaca tccgcgccga cgccgccggc acccacgtgg tggccgacgt
    10921 caccggttac ttcacccgct tcccgaccga ggagttccag ggcggcctgc agaccaagct
    10981 ggtcaccacc gccaactccg tcctcgtcga cctcggcgac ggcggctgca aggagctgac
    11041 gacctgcacc gtcaccacga cggcgccggg gcaggtgatg atcgaggcct gggcccaggt
    11101 cgtcgccggc cataccagcg gcactgagga ccggttcgtc atgcagctcg agacggcggc
    11161 ctccgtgtcg tgccccgagg acgactcggt caacgcctcc gactatgaga tccccgccgc
    11221 gctggggacc aacgccgacg tcgacttcac actgtcccat gcccgggtct tcgcccaggc
    11281 ggcggggcag acgcggacct accggctgag cggccggatg gtgaacggcg cgaatgttca
    11341 agatcaggtc gagaactcgc gggtgctctg caccttcatc ccggactgag gaggccggaa
    11401 agagacgcca tgaagactct ctggcagact tcaatgacgg cggcctgtgc cgccctcttc
    11461 gccgccctcc tcgccggacc tgccgccgcc cagctgcagc cggccgcgga ggacggaccc
    11521 cgggagaagg cgcgggccct gatcgccgag actctcgaga agcgcgtcgt ccgcccagtg
    11581 gcaccggagc gcatcgaggc cgaggcggag cgcatcgtcg cctccctccc ggaggagcag
    11641 gttcagaccc tcgtcgagac ccgggacgcg gctcccgtgc tggaggagcg gacccgggcg
    11701 gccaaagcct cccagccgct cgccgtcatc tcgggcaagg cgctgggtga ctcccaatcc
    11761 gacctggtgt ttgtccccgt ggcgccgtgc cggatcatcg acacccgcca ggtcagcgcc
    11821 ggccccctcg ccgccaacga gacgcgccat ttcctggtcg ccggcaccaa cgccttcctc
    11881 gcccagggag gcaccggcgg cggctgcggg gtcccggacg gcgtgtcgga gcccgcggcc
    11941 ccggcggtgg tgatcaactt tatcgccgtc ggcccgtccg gtcccggcaa cctgcgcgcc
    12001 tgggagtatg gccagccggt cccccgggcc agcgtcatca actacgccaa cgtccccggg
    12061 ctcaacatcg ccaacggcgt cgtggtgccc atcgaagg
//
LOCUS       DASPJI010000757         4702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_792173, whole genome shotgun sequence.
ACCESSION   DASPJI010000757 DASPJI010000000
VERSION     DASPJI010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792173"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VEL74_21305"
     CDS             complement(<1..1356)
                     /locus_tag="VEL74_21305"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HYG65132.1"
                     /translation="MADRRHHLEGIVIPGPADRRPEAVQLELFTHRFAAIARQMGERL
                     RRTAVSTNVKERLDFSCALLDAAGELVVNAPHIPVHLGALGLCVRRLRETLPMEPGDV
                     VVTNHPAFGGSHLPDVTVVTPVFADEGDLLGYVASRAHHAEIGGIRPGSMPPGARRLV
                     EEGVVIPPAYLARGGTPRWEALRRLLLEAPFPSRAVEENLADLRAAAAANHGGAEALA
                     ALAREHGREAVAHYMEALKARAEQRLREALARLPAGRYEAAERLDDGSPLAVRIDISP
                     NLSTSAAAARIDFTGSAGVHAGNLNAPPAVLRSAVIYVLRLLIAEPLPLNEGLLRAVE
                     IHVPEGMLNPPFPDDPAEAPAVVGGNVETSQRLVDTLLKALRLAACSQGTMNNLLFGN
                     ERFGFYETVCGGAGGGPGFGGASAVHTHMTNTRITDPEVLEHRYPVRLERSAIRRGSG
                     GE"
     gene            1406..3304
                     /locus_tag="VEL74_21310"
     CDS             1406..3304
                     /locus_tag="VEL74_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015178624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HYG65133.1"
                     /translation="MPSSKTTSTTRSRTPAGPQRPRHGYPRPQLERDSWISLDGPWDF
                     ALDPAARWRVAREVKWNTKIEVPFSPETPLSGIGDTGFYRRCWYRRTFTAPKLRRGDR
                     LLLHFGAVDWLATVWVNGSLAACHEGGYTPFSADITDLLDPAGEQTIVVRVDDDPADL
                     AKPRGKQDWQLEPHSIWYPRTTGIWQTVWMEKVSSTWIESLRWTPSLDRWELSFEAWV
                     AGEPRDRLRLGVKLFARGQLLADDTYTVVAGEVHRRIALSDPGIDDFRNELLWSPATP
                     TLIDVQLQLWGDRGELLDEARGYTALRSVAIQGDRIVLNGRPYYLRMVLDQGYWPESG
                     LTAPDDDALRRDVELARAMGFNGVRKHQKIEDPRYLYWADRLGLLVWEEMPSAYRFTK
                     RSVHRLTREWMEAVRRDYSHPSVIAWVPFNESWGVPNLPDSQPERNWVRSLYHLTKTL
                     DSSRPVIGNDGWESIATDIIGIHDYDHDPERVSKRYHAEDLLPQLLKRERPGGRLLVL
                     EEHGHRDHPVILTEFGGIAASPNTETTWGYSRAESGQDLAARYRDLLDTVNSLNLFAG
                     FCYTQFADTYQEANGLLYADRTPKFPLEEMAAATRGENADRQRPASVSDTGAGTRHGP
                     EAPPFIEE"
     gene            3326..4486
                     /gene="galK"
                     /locus_tag="VEL74_21315"
     CDS             3326..4486
                     /gene="galK"
                     /locus_tag="VEL74_21315"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HYG65134.1"
                     /translation="MTEQSNPRFEELFGRPAEVRSDAPGRVNLIGEHTDYNGGFVLPI
                     AIPQHTRMELAVRDDARVRAWSANVRKYPLCEYVLGEETKQRGWIDYIQGITWVLRQG
                     GHGFPGFDLRIESDVPLGSGLSSSAAIEVALLRALRRAFALDLDDIQIALLGQKAEND
                     FVGAPVGVMDQMASSLAGEGEALFLDTRSLRYERVLMPAGAELVVINSGISHSHAAGE
                     YRIRRQECERAAEILGVELLRQLEVQDLWRLPALPEPLDRRARHVITENGRVRTAVAA
                     MRERNLPRLGQLLNASHASLRDDYEVSTPEVDRLVEIAQAEPEVFGARITGGGFGGSV
                     VIVARSGTGREVGERVAAAYRESTGRPGTLVVPPPAGLQGALASSRTAESQA"
     gene            complement(4493..>4702)
                     /locus_tag="VEL74_21320"
     CDS             complement(4493..>4702)
                     /locus_tag="VEL74_21320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65135.1"
                     /translation="ENVLAAYRKGTLVSFALLDGAAVLGLVVALLSGNRFYGTVLCAA
                     ALLGMLTRWPRASEVDRLLRGRTSL"
BASE COUNT          720 a         1702 c         1624 g          656 t
ORIGIN      
        1 ctcgcctccc gagccgcggc ggatggcgga gcgctccagg cgcaccggat agcggtgctc
       61 cagaacctcg ggatcggtga tccgcgtatt cgtcatgtgg gtgtgcacgg cgctcgcgcc
      121 gccgaacccg ggtccgcctc cggccccgcc gcagaccgtc tcgtagaagc cgaagcgctc
      181 gttgccgaag agcaagttgt tcatcgtccc ctggctgcag gcggcgagac gcagagcctt
      241 gaggagagtg tccaccagcc gctggctggt ctcgacattg cctccgacca ccgcgggcgc
      301 ctcggcgggg tcatccggaa agggcgggtt cagcatcccc tcggggacgt ggatctccac
      361 cgcccggagc agcccctcgt tcaacggcag gggctcggcg atgagcagcc ggagcacgta
      421 gatgaccgcg cttcgcagca ccgccggcgg agcgttcagg ttgccggcgt ggacgccggc
      481 cgagccggtg aagtcgatgc gggccgccgc cgcggatgtc gacaagttgg gagagatgtc
      541 gatccgcacc gccaggggcg agccgtcgtc cagccgctcg gcggcctcgt agcgcccggc
      601 ggggagccgc gccagcgcct cccgcagccg ctgctcggcc cgcgccttca gcgcctccat
      661 gtagtgggcg acggcctcgc ggccgtgctc ccgcgccagc gccgccagcg cctcggcgcc
      721 gccgtggttg gccgccgccg cggcccgcag gtcggccagg ttctcctcca ccgcccggga
      781 ggggaacggc gcctccagga gcagccggcg gagcgcctcc cagcgcggcg tgccgccccg
      841 ggccagatac gccggcggga tcaccacccc ctcctccacc agccggcgcg ctccgggcgg
      901 catcgagccc ggccggatgc cgccgatctc cgcgtggtgg gcacggctgg ccacgtaccc
      961 caggaggtcc ccctcgtccg cgaagaccgg ggtgaccacc gtcacgtcgg gcaggtggga
     1021 gccgccgaaa gccggatgat tcgtgaccac cacgtcccct ggctccatag ggagggtctc
     1081 gcgcaggcgc cgtacgcaga gccccagggc ccccagatgc accgggatgt ggggggcgtt
     1141 gaccaccagc tcaccggccg cgtccagcag cgcgcaggag aaatcgagcc gttccttgac
     1201 gttggtggac accgcggtgc gccgcagccg ctcccccatc tgacgggcga tggcggcgaa
     1261 gcgatgggtg aagagctcga gctggacggc ttcggggcgg cggtctgcgg ggccggggat
     1321 gacgattccc tccagatgat ggcgacgatc agccattata gttttgaccc ttctggcttg
     1381 gcgattgcaa cgagcgccgg ttgacatgcc gagctccaag accacgtcca cgacccggtc
     1441 caggaccccc gcgggtcccc aacgtcctcg ccacggttat cccagacccc aactcgaacg
     1501 ggactcctgg atctccctcg acggcccctg ggacttcgcg ctcgacccgg ctgcccgctg
     1561 gcgggtggcg cgcgaggtga agtggaacac caagatcgaa gtccccttct cccccgagac
     1621 gccgctgagc ggcatcggcg acaccggctt ctaccggcgg tgctggtacc ggcgcacgtt
     1681 cacggcgccg aagctccgcc ggggagaccg gctgctcctc cacttcggcg cggtggactg
     1741 gctggccacc gtgtgggtga acggctcgct ggccgcctgc cacgaggggg gctatacgcc
     1801 gttctcggcg gacatcaccg atcttctgga ccccgcgggc gagcagacca tcgtggtacg
     1861 ggtcgatgac gaccccgccg acctggcgaa gccgcgcggc aagcaggact ggcagctcga
     1921 gccgcactcc atctggtatc ccaggacgac cggcatctgg cagaccgtct ggatggagaa
     1981 ggtctcctcc acctggatcg agagcctccg ctggaccccc agcctggacc gctgggagct
     2041 gagcttcgag gcctgggtgg ccggcgagcc gcgcgaccgc ctgcgtctcg gagtcaagct
     2101 cttcgcccgc ggccagctcc tggcggacga cacctatacg gtggtggcgg gcgaggtcca
     2161 ccggcgcatc gccctgtccg acccgggcat cgacgacttc cgcaacgagc tgttgtggag
     2221 cccggcgacg ccgacgttga tcgacgtcca gctccagctc tggggcgacc ggggcgagtt
     2281 gctggacgag gcgcggggct acactgcctt gcgctcggtg gcgatccagg gggaccgcat
     2341 cgtcctcaac ggccgtccct actacctgcg gatggtgctc gaccagggtt actggccgga
     2401 gagcggcctc accgcccccg acgacgacgc cctccggcgc gatgtcgagc tggccagggc
     2461 catgggcttc aacggcgtcc gcaagcacca gaagatcgag gacccgcgtt acctctactg
     2521 ggccgaccgc ctcggattgc tggtctggga ggagatgccc agcgcctacc gcttcaccaa
     2581 gcgctcggtg caccgcctga ctcgcgagtg gatggaggcc gtccgccgcg actacagcca
     2641 tccgtcggtc atcgcctggg tgccgttcaa cgagtcctgg ggggtgccga accttcccga
     2701 cagccagccg gagcgcaact gggtccgctc gctctaccac ctgaccaaga ccctggactc
     2761 gtcgcggccg gtgatcggca acgacggctg ggagagcatc gccacggaca tcatcggcat
     2821 ccacgactac gaccacgacc ccgagcgcgt ctccaagcgc taccacgccg aggacctcct
     2881 gccacagttg ctcaagcgcg agcggcccgg cggccggctc ctcgtgctgg aggagcacgg
     2941 gcatcgggac catccagtga tcctcaccga gttcggcggc atcgccgcgt cccccaatac
     3001 ggagacgacc tggggatact ccagggccga aagcggccag gatctggccg cgcgttaccg
     3061 cgatctgctc gataccgtca actcgctcaa cctgttcgcg ggcttctgct acacccagtt
     3121 cgccgacacc tatcaggagg ccaacggcct cctctacgcc gaccgtaccc cgaagttccc
     3181 gctcgaagag atggccgcgg cgacccgggg ggagaacgcg gaccgccagc ggccggcgtc
     3241 ggtgagcgat accggggccg gcacgcggca cggcccggaa gcgccgccgt tcatcgagga
     3301 gtaaggcccg ggatggaggt attccgtgac tgagcagtct aacccccgat tcgaggagct
     3361 gttcggccgt cccgccgagg tccggtccga cgcgccaggg cgggtcaatc tgatcggcga
     3421 gcataccgac tacaacggcg gcttcgtgct gcccatcgcc atcccccagc acaccaggat
     3481 ggagctggcc gtgcgggacg acgcccgggt ccgcgcctgg agcgccaatg tgcgcaagta
     3541 ccccctgtgc gagtacgtcc tcggcgaaga gaccaagcag agggggtgga tcgactacat
     3601 ccaggggatc acctgggtgc tgcggcaagg ggggcacggc tttccggggt tcgacctgcg
     3661 catcgagtcg gacgtgccac tggggagcgg cctctcctcc agcgcggcca tcgaggtcgc
     3721 ccttctgagg gcgctccggc gggccttcgc cctggacctc gacgacatcc agatcgccct
     3781 cctgggccag aaagcggaga acgacttcgt cggcgcgccg gtgggggtca tggaccagat
     3841 ggcgtcgagc ctggccggcg agggcgaggc gctcttcctc gacacccgca gcctccgcta
     3901 cgagcgcgtg ctcatgccgg ccggcgcgga gctggtggtc atcaattccg ggatctctca
     3961 cagccacgcc gcgggcgagt accggatccg gcgccaggag tgcgagcggg ccgccgagat
     4021 cctcggcgtg gagctgctcc gccagctcga ggtccaggat ctctggcgcc tgccggcgct
     4081 ccccgagccc ctagaccggc gcgcccgcca tgtgatcacc gagaacggcc gcgtccgcac
     4141 cgccgtcgcg gccatgcggg agaggaacct gccgcgcctc ggccagctcc tcaacgcctc
     4201 ccacgcgtcg ctgcgcgacg attacgaggt ctccaccccc gaggtcgacc ggctggtcga
     4261 gatcgcccag gcggagcccg aggtcttcgg cgcccgcatc accggcggcg gcttcggcgg
     4321 ctcggtggtg atcgtcgccc ggagcggcac cggccgtgag gtgggcgagc gggtcgcggc
     4381 ggcctaccgg gagagcacag gccggcccgg gacgctggtg gtccctccac ccgccgggct
     4441 ccagggagcc ctcgcctcct cccgcaccgc cgagagccag gcctgaggct gcttacagcg
     4501 aggtccgccc gcgcagcagg cggtcgacct cgctggcacg aggccagcgg gtgagcatgc
     4561 ccaggagggc ggcggcacag agcacggtgc cgtagaaccg gttcccggag agcagggcca
     4621 ccacgaggcc gagcacggca gccccatcca gcaacgcgaa gctcaccagc gtcccctttc
     4681 gataggcggc gaggacgttt tc
//
LOCUS       DASPJI010000758         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_272058, whole genome shotgun sequence.
ACCESSION   DASPJI010000758 DASPJI010000000
VERSION     DASPJI010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272058"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VEL74_21325"
     CDS             <1..302
                     /locus_tag="VEL74_21325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HYG65136.1"
                     /translation="FEWACFTAHQAAVKAVFRARGGSVRGHALLHLLRQLPGDLSVSE
                     DLLHSARVLDRYYIEARYPDGFPSGSPMEYFDQTLATEAIDAADTIFRFCSGHLD"
     gene            256..630
                     /locus_tag="VEL74_21330"
     CDS             256..630
                     /locus_tag="VEL74_21330"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYG65137.1"
                     /translation="MLRTQSSGSVRVTWIDRPALLRELEERAARLGRDHPEIAEVVLF
                     GSVARGSATPESDIDILLIVDETGVPFLLRADPYRDVFADLPFDVFPLAYTQSERERM
                     LREGNLFLCSAFEDAVYLYRRA"
     gene            complement(635..1822)
                     /locus_tag="VEL74_21335"
     CDS             complement(635..1822)
                     /locus_tag="VEL74_21335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65138.1"
                     /translation="MRADAARADRNAAIRGAARSWRRAGAIDGATLAAIEAEVPDDRV
                     RVGPVFRVLLFVFTLIAITAAFGFVAMFFGADEEQGVGWLALFWGIALAVATEVQTGP
                     LRRAQGGTEAGTSFAMLCFLQGGTAWLLFDFGNLTPDGGTLAFFFGLGAVLCAAAAWR
                     WGFPLYAAAAMAWLLLALAVAPQGRLLWIAVPLALAPVLLRLSDSPRLPPAHRASCAA
                     ALVVGLAGLYLAVHYGSVESRWVEAMGGRVPLLEIPVGAVTEFLSRVGTALVPPAFLA
                     LGLWRRRRLLLLLGLAAGAASLATWYLQAAPAPLWVFLGVCGLVTLAAALAVRRLLDA
                     APDKERWGFTAEPLFDDPEAGERLEAAASVVTLTPEARAAQEPGFKGGGGEFGGGGAS
                     GSW"
     gene            complement(1819..>2497)
                     /locus_tag="VEL74_21340"
     CDS             complement(1819..>2497)
                     /locus_tag="VEL74_21340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65139.1"
                     /translation="FSWERVPSPGLAFDYVLLLGVLLAAADLAYVEARFTPLGPNWPW
                     HLLLVSLFAAAMAVRFDSRIVFSLALATFAAWRGVSVAHFGAGLLDTRDDLVRWNAIG
                     CGVLFVVLGLVLARAGRKAHFEPAAVHLGCLLVLGGLASGLGDRGWAGWAAALLLAGA
                     GLAAWAYGTRRFPLFAYGVLGGYLGLTRFVFEGVEALGCFWIAFTSILMILGLVAVQR
                     RMREPHR"
BASE COUNT          412 a          927 c          894 g          264 t
ORIGIN      
        1 atttcgagtg ggcgtgcttc actgcgcacc aggcggcggt gaaggcggtg ttccgggcgc
       61 gaggcggcag cgtccgcggg cacgcgctgc tccatctgct ccggcagctc ccgggcgatc
      121 tctccgtctc cgaggatctc cttcactcgg ccagggtgct ggaccgttac tacatcgaag
      181 cccgttaccc cgacggtttt cccagcggat caccgatgga gtacttcgac cagaccctcg
      241 ctaccgaggc catcgatgct gcggacacaa tcttccggtt ctgttcgggt cacctggatt
      301 gaccgccccg cgctcctgcg ggagctggag gagcgggcgg cacggctcgg ccgggaccat
      361 ccggagatcg ccgaagtcgt cctcttcggg tccgtcgccc gcggcagcgc cacccccgag
      421 agcgatatcg acatcctcct gatcgtcgac gagaccggcg tgcccttcct cctgcgcgcc
      481 gatccctacc gggacgtgtt cgccgacctg ccgttcgacg tcttccctct cgcctatacc
      541 cagagcgagc gggagcggat gctgcgggag ggcaatctct tcctgtgctc ggccttcgag
      601 gacgcagtct acctctatcg ccgggcctga cggatcacca cgacccgctc gccccgccgc
      661 cgccgaactc cccgcctccc cccttgaagc ccggctcctg agcggcccgc gcctcgggcg
      721 tcagggtcac caccgaagcg gcggcttcga ggcgctcgcc cgcttccggg tcgtcgaaga
      781 gcggctcggc ggtgaagccc catctctcct tgtccggcgc ggcgtcgagg aggcggcgga
      841 ccgccagggc ggcggcgagg gtgacgagcc cgcagacgcc gaggaacacc cagagcgggg
      901 cgggagcggc ctggagatac caggtggcga gggaggccgc accggccgcc aggcccagca
      961 gcagaaggag acgccggcgc cgccagaggc ccagcgccag gaacgccggc ggcaccaggg
     1021 ccgtgcccac cctggacaga aactccgtga ccgcgccgac ggggatctcc agcaggggga
     1081 cccggccgcc catggcttcg acccacctcg attccacgga gccgtagtgg acggcgaggt
     1141 agagcccggc gagccccacc accagcgccg ccgcgcagga ggcgcgatgc gcgggcggga
     1201 gccgcggcga gtccgacagc cgcaggagca ccggggcgag cgccaggggg acggcgatcc
     1261 agaggaggcg cccctgggga gcgacggcca gggccagcag gagccaggcc atggccgccg
     1321 ccgcgtagag cgggaagccc cacctccagg ccgcggcggc gcagaggacc gcgccgagcc
     1381 cgaagaagaa cgccagggtc cccccgtccg gcgtcaggtt cccgaagtcg aacagcagcc
     1441 aggcggtgcc cccctgcagg aaacagagca tcgcgaacga ggtgccggcc tccgtccctc
     1501 cctgggcgcg ccgcagcggc ccggtctgca cctcggtggc cacggcgagc gcgatccccc
     1561 agaagagggc cagccatccc accccctgct cctcgtcggc gccgaagaac atcgcgacga
     1621 agccgaaggc cgccgtgatg gcgatcaggg tgaagacgaa gaggaggacc cggaagaccg
     1681 gcccgacccg cacccggtcg tccggcacct ccgcctcgat ggccgccagg gtggcgccgt
     1741 cgatggcccc ggcccgccgc caggagcggg cggcgccccg gatagccgcg ttgcggtcgg
     1801 cgcgggcggc gtcggccctc atcgatgcgg ctcccgcatc cggcgctgga cggcgaccag
     1861 ccccaggatc atcaggatgg aggtgaaggc gatccagaag cagcccagcg cctcgacccc
     1921 ctcaaagacg aaccgcgtca gtcccaggta cccgcccagg accccatagg cgaagagggg
     1981 aaagcggcgg gtgccgtagg cccaggcggc gagcccggcg cccgccagca gcagcgccgc
     2041 cgcccacccc gcccagccgc ggtcgccgag gcccgaggcg agcccgccga ggaccagcag
     2101 gcaccccagg tggaccgccg cgggctcgaa gtgggccttg cggccggcgc gtgccaggac
     2161 caggcccagg accacgaaca ggacgccgca gccgatggcg ttccagcgca ccaggtcgtc
     2221 ccgggtgtcg aggagcccgg cgccgaagtg ggcgaccgac actccgcgcc aggcggcgaa
     2281 ggtggcgagg gccagcgaga agacgatgcg cgagtcgaag cgcaccgcca tcgccgcggc
     2341 gaagagggag accaggagga gatgccaggg ccagttcggg ccgagcgggg tgaaccgcgc
     2401 ctccacgtag gccaggtcgg ccgccgccag gagcaccccc aggaggagga cgtagtcgaa
     2461 ggccaggccg ggcgacggca cccgctccca ggagaac
//
LOCUS       DASPJI010000759         2910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_272095, whole genome shotgun sequence.
ACCESSION   DASPJI010000759 DASPJI010000000
VERSION     DASPJI010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272095"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VEL74_21345"
     CDS             <1..682
                     /locus_tag="VEL74_21345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65140.1"
                     /translation="FLVAENAGGTVEILDAAGEPLVALPARTRPAAVVAALEHLARYR
                     NVQELENKDPKSDLYDKLAVSLERPPGPERFPKLPPAVRPGDWVRLSIQNNSVQELNI
                     AVLNLQADWSIRQAYPNAAFLPLDSGAVERIPFEVRWPFRLPRGRDLFKIFAVSGYLD
                     CRWLELPALGEPPLPPPGDRGSLAPGSLTDLFAALTGEPPPVRALVPERQPEHSWTVA
                     DLVIEVSR"
     gene            682..>2910
                     /locus_tag="VEL74_21350"
     CDS             682..>2910
                     /locus_tag="VEL74_21350"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HYG65141.1"
                     /translation="MPDFSIDLAVVIGIDDYSDTVGRLRTPVEDAKVIADLLASRFGY
                     SVILFCDSAAGLKGLRQLLHADLRVPEGSRLLFYFAGHGQASDGPELEGPRGSLVPHG
                     GTADVETHLSMDELYKALTGLPCRHLLVILDCCFAGSFRWAGRRPLYAPGDLLYEERY
                     RHWVRHPAHWFLSSAAHDQYALDTLDRRRGRSDLHSPFAAAVLDGLKGKADLLPPGGD
                     GVVTLAELYTYVQDRLSPHQTPLLSQLPGQTRGEYVFRAPGRPLPSAQSVVLTRETNP
                     YRGLKPYRAEHAELFFGRGTAARRLLRHVRTHGLTVVLGPSGSGKSSLVQAGLLPRLR
                     RWTGRGTRPAAGWKVLLPLRVTADPFFDLNKTLAAAGGPPPPSVEELRRDARTLTAWA
                     AAWRAAHPCERLLLFLDQAEELVTRVPRSGAEASGEIPVAAAGLLAGLAALLRGGEGR
                     LRVLLTLRSDFEPDLDEGPLTGLWKTGRFLVPALAREELREAVEGPAARKALYFDDPR
                     MVERLVDEVVGMPGGLPLLSYALSQLYLAYIVESRGDRLLTAADFAKIGGDAELDGPG
                     AAGGIVRVLRKAADDAVRSLPDDAHRETLWLVLLRMVNLVGVRPTRRQVPASELDYPD
                     PAENRRRDEVLARLVDARLLVKDAGVVEPAHDELLVAWPQLAGQIEAAKVRLPVHRRL
                     SETAVEWSTAGRRRRDLDLRLLGLVETAGLPTAWLNRAESEYVTEARQERRRRIARRW
                     TLA"
BASE COUNT          384 a         1035 c         1012 g          479 t
ORIGIN      
        1 cttcctggtg gcggagaacg ctggaggcac cgtcgagatc ctcgacgctg ccggcgagcc
       61 gctggtggcg ctgcccgccc gcacccgacc ggcggcggtg gtggccgcgc tcgagcacct
      121 ggcccggtat cgcaacgtcc aggagttgga gaacaaggat cccaagtctg atctttacga
      181 caagctcgcc gtctctctcg agcggccgcc ggggcccgag cggttcccga agctgccgcc
      241 cgcggtgcgg cccggcgact gggtccgtct gagcatccag aacaactcgg tgcaagagct
      301 caacatcgcc gtcctgaacc tccaggcgga ctggagcatc cgccaggcct acccgaacgc
      361 cgccttcctt cccctggaca gcggcgccgt cgagcgcatc ccttttgagg tgcgctggcc
      421 cttccgtctg ccccggggcc gggacctgtt caagatcttc gctgtctccg gctacctgga
      481 ctgccgctgg ctcgagctcc ctgctctcgg agagccgccg ctgccgccgc ccggcgacag
      541 ggggagtctc gctcccggct cgctcacgga cctgttcgcc gccctgaccg gcgagccgcc
      601 cccggtgcgc gccctcgtcc ccgaacggca gccggagcac tcctggaccg tcgccgacct
      661 ggtgatcgag gtgagccgct gatgcccgac ttctccatcg acctggcggt ggtgatcggt
      721 atcgatgact acagtgacac cgtggggcgc ctgcggacac cggtagagga tgccaaggtc
      781 atcgctgatc tactcgccag ccgtttcggc tacagcgtaa tcctgttctg cgattccgcg
      841 gcaggcttga agggactgcg gcagctgctg cacgcggatc tgcgggtgcc ggagggaagc
      901 cggcttctct tctatttcgc gggccatggg caggcatcgg acggccccga gctggagggg
      961 ccgcgaggct ccctggtccc ccacggcggc accgcggacg tcgagaccca tctgtcgatg
     1021 gatgagctgt acaaggccct gaccggcttg ccctgtcggc acctgctggt gatactagac
     1081 tgctgcttcg ccggctcctt ccgctgggcc ggacggcgtc ccctctatgc gccgggagac
     1141 ctgctgtacg aggagcgcta ccggcactgg gtacgccatc ctgcccattg gttcctgtcg
     1201 tcggcggccc acgaccagta cgccctcgat accctcgacc ggcgccgcgg ccgatcagat
     1261 ctccattcac ccttcgccgc cgccgtgctc gacggactca aggggaaggc cgatctcctg
     1321 ccgcccggcg gcgacggcgt ggtgacgctg gccgagcttt acacctacgt ccaggaccgg
     1381 ctcagccctc accagacgcc gctgctgtca cagctgccag ggcagacgcg gggggagtac
     1441 gtcttccgcg cgccgggccg gccgctgccg tcggcacagt ccgtggtcct cactcgggag
     1501 accaaccctt accggggctt gaagccttac cgcgctgagc acgcggagct gttctttgga
     1561 cgcggcactg ccgctcgccg cctgctgcgc cacgtccgga cgcacggact gacggtggtg
     1621 ctgggacctt cgggcagtgg caagtcgagc ctggtccagg ccggactgct gccccgcctg
     1681 cgtcgctgga ccggccgcgg gacccgcccc gctgcgggct ggaaagtcct gctgccacta
     1741 cgcgtcacgg cagacccgtt cttcgatctt aataagacct tggctgcggc tggcggtccg
     1801 ccccccccct ccgtcgagga gctgcgccgt gacgcccgga cgttgaccgc gtgggcggcc
     1861 gcctggcgcg cggcacaccc ttgcgagcgg ctgctccttt tcctggacca agccgaggaa
     1921 ttggtgactc gcgtgccccg gagcggcgcg gaggcttccg gcgagatccc cgtggcggcg
     1981 gccggtcttc tggccggcct cgcggcgctg ctgaggggcg gtgaagggcg gctgcgggtc
     2041 ttgctgaccc tgcggtccga cttcgagccc gacctcgatg aaggaccgct caccggcctc
     2101 tggaaaacgg ggcggttcct tgtccccgcg ctggcccggg aggagctgcg cgaggcggtg
     2161 gaagggccgg ctgcccgcaa agccttgtac tttgacgacc cgcggatggt cgagcggctg
     2221 gtggacgagg tggtgggtat gccggggggg ctgccgctgc tctcctatgc tctcagccag
     2281 ctttacctcg cctacatcgt cgagtcccgt ggcgatcgtc tcctcaccgc cgccgatttc
     2341 gccaagatcg gcggggatgc cgagctcgac ggtcccggtg ccgcgggcgg gatcgtccgc
     2401 gtcctgcgca aggcagcgga tgacgccgtc cggagcctgc cggatgacgc gcaccgggaa
     2461 acgctctggc tggtgctcct gcgcatggtc aacctggtgg gggtccggcc gacccggcgt
     2521 caggtgccgg ccagcgagct ggactatccg gacccggccg agaaccggcg gcgggacgag
     2581 gtgctggccc ggctcgtcga cgcgcggctg ctggtcaagg atgcgggcgt cgtcgagccg
     2641 gcccatgacg agctgctggt ggcttggccg cagctggcgg ggcagatcga ggccgcgaag
     2701 gtccgcttgc cggtccaccg ccgtctgagt gaaaccgccg tggagtggtc gaccgccggc
     2761 cggcgccgcc gggatctcga cctgcgtctc ctgggtctcg tcgaaaccgc cggactgcct
     2821 actgcctggc ttaaccgagc cgagagcgag tacgtcaccg aggcccgcca ggagcggcgc
     2881 cggcggatcg cgcggcgctg gaccctggcg
//
LOCUS       DASPJI010000760         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_726044, whole genome shotgun sequence.
ACCESSION   DASPJI010000760 DASPJI010000000
VERSION     DASPJI010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726044"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VEL74_21355"
     CDS             <1..387
                     /locus_tag="VEL74_21355"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG65142.1"
                     /translation="DGAVIETADLRDADFPTARAVLLLDVLHYLPAAAQEELLARAVR
                     ALEPGGVLFIRDADAAAGWRFTATRIQERLATMARRQFRQRFHYRSTAEWRALLAAYG
                     LEVSSEPMSMGTPYANVLIQARRPSL"
     gene            complement(599..919)
                     /locus_tag="VEL74_21360"
     CDS             complement(599..919)
                     /locus_tag="VEL74_21360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65143.1"
                     /translation="MYAGAPSYEDTGIVRTVFDAGWREHDEELTFSTAFVQPERFRFE
                     YREPADGTRYVIGSEGGEVKSWWTLRPEVEIEEHSVQGEEAAEVTTTISYHPRIGHAV
                     AGEK"
     gene            1277..>2536
                     /locus_tag="VEL74_21365"
     CDS             1277..>2536
                     /locus_tag="VEL74_21365"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HYG65144.1"
                     /translation="MRPHLKVSVIPLVLFFIFTLLTAVATSAASPIRYGKISWCFAPY
                     YPNYEPVYIAFEVELAYAYTGQSVGDTVTETLHYDGGATTPVVLTVTEVQPAQGWVRA
                     VGNVRPLVSMPHTYAWLDTCCRDGDLNNRSGENFTVSSLVNIWAEWSCSPETGVPPIV
                     WLAGGLGDTFQIPIPYRRGTDSAEPLCSLTGDGLNPDGMTIDPDRCVITWTPTTGDPS
                     KLWTAQVEVGDSFGSVPYTSTGLDFLLGLDDAKPTCLLQSVDPGPPTHLEVFIQDSRS
                     GVASVQVLESANTSVNIPSFLPGTTGELTVVGTKIDQSRSARLRLRVTDLAGNATECD
                     PILTEETRETGAPQTSTHTGIPPEEHVVTVYNGDPGLQSLDIDVNGRKFKMNGLRTGE
                     ERTLDVAAAMPAAGMSTITLTTRSKPGG"
BASE COUNT          429 a          944 c          769 g          394 t
ORIGIN      
        1 gacggcgcgg tcatcgagac cgccgacctg cgggacgcgg acttccccac cgcccgcgcc
       61 gtcctgctgc tggacgtcct ccactacctc cccgccgccg cccaggagga gctcctggcc
      121 cgcgccgtcc gcgccctcga gccgggaggc gtcctcttca tccgcgacgc cgacgccgcc
      181 gccggctggc gcttcaccgc gacccgtatc caggagcgcc tcgccacaat ggcacgccgc
      241 caattccggc aacgcttcca ctaccggagc accgccgagt ggcgcgccct cctggccgcc
      301 tacggcctgg aggtctcctc cgagccgatg agcatgggca ccccatacgc caacgtgctc
      361 atccaggcgc ggcgcccgag cttatagcca gacatcgatt cagcatgtct gaaggcggcc
      421 tcctatccag gaggctgtgc agtagaaagg acgtgcaaca tgaagaagaa gaacacacgg
      481 accgagcgca tcctgggccg ccgcctcgcc gttcaccttc ccgccggatc gcgacaagat
      541 ctgctgatcc tcgccggccc cggtctctcc ggccggggcc ctttccagga ttacccggtc
      601 acttctcccc ggcaacggcg tggccgatcc tcggatgata cgagatcgtg gtcgtgacct
      661 cggcggcctc ctcgccttgc acggagtgct cctcgatctc cacctcggga cggagcgtcc
      721 accaactctt cacctccccg ccttccgacc cgatgacata gcgggtcccg tccgcgggtt
      781 cccggtactc gaaccggaac cgctccggct ggacgaacgc cgtagaaaag gtcagctcct
      841 cgtcgtgctc gcgccagccg gcgtcgaaga cggtcctgac gatcccagtg tcctcgtagg
      901 agggggcgcc ggcgtacacc gcctgcgccc aggccaggat ctcccgggcc gtgatcccat
      961 cggccggggc ggaccgggag acctcgacgc cctcttcttc cctcgcagcg gccgcggatg
     1021 gccgggccgg gaggaggtcg gccacaggtc gaggaagagg aggcagggga tcaggacgaa
     1081 gagccggctg cgcatggtgt caccactcct tggaaagcac tgctccggga acgacggctt
     1141 cgaagagaag tgtatcgggg ggccgatccg tttcagcgca ccgggctcct acaaccgtat
     1201 gctgaagtgc gccttttgct acggtacgag gcgtgcaccc acggccgagt gatgcccaca
     1261 caggaggaga tcgtctatgc ggccacatct caaggtcagt gtcattcctc tggtcctctt
     1321 ctttatcttc actctcctga ccgcggtcgc cacatcggcg gcaagcccga tccggtacgg
     1381 caagatcagt tggtgctttg ccccctacta tccgaactac gagcccgtct acatcgcctt
     1441 cgaggtcgag ctggcctacg cctacaccgg ccagtcggtg ggtgacaccg tcaccgaaac
     1501 tctccattac gatggcgggg ctaccacccc cgtcgttctg accgtcaccg aggtccagcc
     1561 cgctcaggga tgggtgcggg ccgtgggaaa cgtccggccc ctcgtctcga tgccccacac
     1621 gtacgcctgg ctggacacct gctgccgcga cggcgacctg aacaaccgca gcggcgaaaa
     1681 cttcaccgtc tcctcgctgg tcaacatctg ggcggagtgg tcctgctcgc ccgagacggg
     1741 cgttcccccg atcgtctggc tggccggcgg cctcggcgac accttccaga tccccatccc
     1801 ctaccggcgg gggacggaca gcgcggagcc tctctgcagc ctcactggtg acggcctcaa
     1861 tccggacggc atgaccatcg accccgatcg gtgcgtcatc acctggaccc ccacgaccgg
     1921 cgatccgtcg aagctctgga cggcgcaggt cgaagtgggc gactcattcg gcagcgttcc
     1981 ctatacctcg accggcctgg acttcctgct cggcctcgac gatgccaagc cgacctgcct
     2041 gctccagagc gtcgatcccg gtccgcccac ccatctcgag gtcttcatcc aggacagccg
     2101 gagcggcgtc gccagcgtcc aggtgctcga gtcggccaat acttcagtga acatcccctc
     2161 gttcctgccg ggcacgaccg gcgagctcac cgtcgtgggc accaagatcg accagagccg
     2221 cagcgcgcgc ctccgcctgc gggtgacgga cctggcaggc aacgccaccg agtgcgaccc
     2281 gatcctcacc gaggagacgc gggagaccgg cgcgcctcag accagcaccc acacgggcat
     2341 cccgccggag gagcacgtgg tcacggtcta caacggcgac cccggcctcc agtcgctcga
     2401 catcgacgtc aacggcagga agttcaagat gaacggcctg cggaccggag aggagcgcac
     2461 cctcgacgtg gccgcggcca tgcccgcggc aggcatgtcg accatcaccc tgaccacccg
     2521 cagcaagccg ggcggg
//
LOCUS       DASPJI010000761         5688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_852231, whole genome shotgun sequence.
ACCESSION   DASPJI010000761 DASPJI010000000
VERSION     DASPJI010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852231"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1709)
                     /locus_tag="VEL74_21370"
     CDS             complement(<1..1709)
                     /locus_tag="VEL74_21370"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HYG65145.1"
                     /translation="MRRGVSPLVLLLLVLAGAAVPALPAGAAAVPPSCRTVDPYLAAG
                     DRAAKDEKVEGPAAFAAFRKAIGTAARQAGVKTLAGQLAALEPGKPVAAESADLAACL
                     LRHWVRVRYGQRMVDDLREMVGFRTFAETGRENWQAPEFLRQRDWLRGRAEAMGFAFK
                     SYDGRMEEITFAGPEPILALLTHGDVQAVEGQAWSSPPWEGRLLPDGKIVGRGTEDDK
                     GPIVAILYSMAAVRDAGWPLSSTLRLLIANAEESSWQEIPYYLERAPMPDMTFGVDAT
                     YPVTFAQKGWGLLSFRAPLEQGTEPPAARWRIERVTGGVAPSIIPGEAEALVAPSGNG
                     QGGTDAEAELRRLAEAWAAAHPPARLQVGREGDLLKVTSRGRSGHSAWPENAQNALTA
                     LTTFLSTLQPRLDPWGALISFLGSTIGLETDGSSMGIAHFDPAMGRLTATLNVMGVND
                     DGEPFARVNIRPPQGVTVEGVRERVAERVDAFERRTNYLLSGEVQIPNLPHVAPVEGK
                     LVSTLLGVWKEVTGQKGEPVAVSGGTQARLFKGGVDFGPALSMDVYRGHGPDEYLTVA
                     ELHR"
     gene            1832..3043
                     /locus_tag="VEL74_21375"
     CDS             1832..3043
                     /locus_tag="VEL74_21375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65146.1"
                     /translation="MRKAPLAAAPLVMAACLALQAWSSPAAAQPVRITGRVSPAEARI
                     ELHPAHRTFEERLRGLTGGPEPASLATAAPRPDGTFEIAAPGPGFYRIVLNAEGLVPL
                     ERFLSPLLEPAALAPARLTRGAPLEVQVLGPAGTPEKGIEVRASGSAAPVVGRSEKVQ
                     AWTPAEVRGVTGEDGRVRFPRRRGDRAFVSVTTLCFVEWLEGVDGTSATLRLRQRPAR
                     VVKVLRPDGRPAPGTLVSLDASHPLGVTGADGRLALPVLDQGSPELLLEGPGGLSTTL
                     RWPAPGSGPVTATLAPPRLVTGGVLDVLTSAPLAGALVWSQDMPPVRTGARGEFELRL
                     PLAQQPAVAAAVAGYLTAWQPVPPSVGSEKTALLLKLAPSATLAGVAVDDGGRGPDRS
                     RAPRLVPPHSA"
     gene            3236..5362
                     /locus_tag="VEL74_21380"
     CDS             3236..5362
                     /locus_tag="VEL74_21380"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HYG65147.1"
                     /translation="MDGEGRPVAGATLSLLEVERTFMIEYGPGRATTDAAGRFRLPHL
                     PPRPFDLLASASGFGPLRVPGLVPPASRNPGGAVLDLGDVVLPPCVAIEGRVTDRRGS
                     PLAEANVGLAETIRNAEPSPGSQVTTGPDGRFRLADLRQGERYDLWVQHQGHVSAHVP
                     GVEAPTDEPLRVELRPARVLAGRVVGPAGEPVPYASLLDFEENGAGQGDGLSSYGSQA
                     STLTRTDAQGRFRADGVEPGTLNLEVKAPEYKTRRLRGLVIPEESDVENLEIVLERGA
                     TVTGRVLDARGEPVAGADVSATPMEAGRGIGREDHSASSETDGEGRYRLGGLDPGSHR
                     ITANRYAEGQHTETEILVRPGENVVNLAFRRITEVSGRVVDESGEPISNASVSLGGAH
                     DAKGVRSGPGGLFHFPAVEDGDYQLSGGGSGFIGPVEPVAVEVDGRPVRDLELRLRRG
                     GTLLTGRLLGLAPGELERVTVAAQAEGTPWPRPGTVSRDGTYRLPDLEPGTWEVTAAL
                     SSGRRARASIRLEPGQERAVLDLEITSGLALAGRVLADGAPLPGVRILLSRQRGRGAE
                     TTTVHDGRFRLEGLEPGAYVLLATHSATGLGTAQAVDLSQDRDLLVEIASGAVRGQVE
                     LPPGEPAAGLDIFLEGTDPALGVSFLGPSVQTDAQGSFELPRVAAGRYRLTVRRRGSP
                     LATAEVRVAPGEAATVRVEPGAGGVH"
BASE COUNT          727 a         2153 c         2031 g          777 t
ORIGIN      
        1 cggtgcagct cggccacggt caggtactcg tccggtccgt gcccccggta gacgtccatg
       61 gagagggccg gcccgaagtc cacgcccccc ttgaacagcc gggcctgagt ccccccggac
      121 actgccacgg gctccccctt ctggcccgtg acctccttcc agacgcccag cagcgtcgac
      181 accagcttcc cctccaccgg cgcgacgtgc ggaaggttgg ggatctggac ctcccccgac
      241 aggagatagt tggtgcgccg ttcgaaggcg tcgacccgct cggccacccg ctcccgcacc
      301 ccctccaccg tgaccccctg cggcggccgg atgttgaccc gggcgaacgg ctcaccgtca
      361 tcgttgacgc ccatcacgtt cagcgtcgcg gtgagccgtc ccatggccgg gtcgaagtgg
      421 gcgatcccca tggaggagcc gtccgtctcc aggccgatgg tgctccccag gaacgagatc
      481 aatgcccccc aagggtcgag ccgcggctgc agggtgctga ggaaggtggt cagggcagtg
      541 agggcgttct gcgcgttctc cggccaggcg gagtggccgc tgcgcccccg cgacgtcacc
      601 ttcaggaggt cgccctcccg gcccacctgc aggcgcgccg gcggatgggc ggcggcccag
      661 gcctccgcca gccgccgcag ctcggcttcg gcatcggtcc ccccttggcc gttccccgag
      721 ggcgccacca gcgcctcggc ctcgccgggg atgatggacg gcgccacgcc gccggtgaca
      781 cgctcgatcc gccagcgggc cgccggcggt tcggtcccct gctccagcgg cgcccggaac
      841 gacagcagcc cccacccctt ctgcgcgaag gtcaccggat aggtggcgtc gacgccgaag
      901 gtcatgtcgg gcatcggcgc ccgctccagg taatagggga tctcctgcca gctcgactcc
      961 tcggcgttgg cgatcaggag gcgcagcgtc gacgagagcg gccagccggc gtcgcggacc
     1021 gccgccatgg aataaaggat ggccacgatc ggccccttgt cgtcttcggt gccgcggccg
     1081 acgatcttcc cgtccggcag cagccgcccc tcccacggcg gcgatgacca ggcctgcccc
     1141 tcgaccgcct gcacgtcgcc atgggtgagc agcgccagga tcggctccgg gcccgcgaag
     1201 gtgatctcct ccatccggcc gtcgtagctc ttgaaagcga agcccatcgc ctcggcccgg
     1261 ccgcgcagcc aatcccgctg gcggaggaac tcgggggctt gccagttctc cctccccgtc
     1321 tcggcgaacg tgcggaaacc caccatttcc cgcaggtcgt ccaccatgcg ctggccgtag
     1381 cggacccgca cccagtgccg gagcaggcag gcggccaggt ccgccgactc cgccgccacc
     1441 ggtttgcccg gctccagcgc cgcgagctgc ccggccagcg tcttgacccc ggcttggcgc
     1501 gccgccgtgc cgatggcctt gcggaaggcg gcgaaggcgg cggggccctc taccttttca
     1561 tccttggcgg cgcggtcgcc ggctgcgagg tacgggtcca ccgtccggca ggatggcggc
     1621 acggccgcgg cacctgcggg gagggcaggc acggcggcgc cggccaacac gagaagcagc
     1681 aacaccaggg gcgagacacc tctgcgcatg agacctccgg aatccgagaa tcgaaagggc
     1741 gcggcaggtt tctatcacaa ggcggccggt ccgcagagac ctgaaatact cttccggatt
     1801 cgtccatact gaatggcgag gaggaccgag catgaggaag gctcccctgg ccgctgcccc
     1861 cctggtaatg gcagcctgtc tggcgctcca ggcctggtcc tcgccggccg cggcgcagcc
     1921 ggtccggatc accggcaggg taagccctgc ggaggcgagg atcgagctgc atccggccca
     1981 tcggacgttc gaggagcgtc tccgcggtct caccggagga ccggagccgg cgtccctggc
     2041 cacggcggcg ccgcgccccg acggcacctt cgagatcgcc gcgcccggtc ccggcttcta
     2101 ccggatcgtc ctcaacgccg agggcctcgt gcccctggag aggtttctct ctccgctctt
     2161 ggagccggcg gcgctggcgc cggcgcggct cacccggggc gcgccgctgg aggtccaggt
     2221 gctgggaccc gccggcacac ccgagaaggg gatcgaggtt cgggcgagcg gctcggccgc
     2281 acccgtcgtg ggccgtagcg agaaggtcca ggcctggacg cccgccgagg ttcgcggcgt
     2341 caccggcgag gatggccggg tgaggttccc ccggcggcgg ggcgaccgcg cgttcgtctc
     2401 cgtcaccacc ctctgcttcg tcgagtggct cgaaggcgtc gacggcacct cggccaccct
     2461 ccggctgcgg cagcggccgg cccgggtggt aaaggtcctg cgccccgatg gccgaccggc
     2521 gcccggcacc ctggtgtcgc tggatgcctc tcacccgctg ggggtgaccg gcgccgacgg
     2581 tcgtctggcg ctgcccgtcc tggatcaagg gtcacccgag ctgctgctgg aaggcccggg
     2641 cgggctctcg accaccctgc gctggccggc acccggcagc gggccggtga ccgccaccct
     2701 ggcgccgccg cgcctcgtca ccggaggcgt cctcgacgtg ctgacgtcgg cccccctggc
     2761 cggcgccctg gtctggagcc aggacatgcc gccggtccgc accggagccc ggggtgagtt
     2821 cgagctgcgc ctccctctcg cgcagcagcc cgcggtcgcc gccgcggtgg cgggctacct
     2881 cacggcttgg cagcctgtgc cgccctccgt cggctcggag aagaccgcgc tgctgctgaa
     2941 gctggccccc tcggccaccc tcgccggcgt ggcggtggac gacggcggcc ggggcccgga
     3001 tcgaagccga gccccacggc tcgtcccgcc tcattccgcg tgacacgccg gccaagagtg
     3061 gtcccgacgg ccgtttcgtc ctccaggggc tcgccgccgg cgggagctac gagctccggg
     3121 ccactcatca gagcttcgcc ccgacgacgg caccggccac ggctcctgcc ccaggccggc
     3181 cggcgcccga ggcgcgctcg tcttctccgc cggctggacc gtcacgggcc ggctggtgga
     3241 cggggagggg cgcccggtag ccggggccac cctgtccctg ctcgaggtgg agaggacctt
     3301 catgatcgag tacggccccg gccgcgccac caccgacgcc gccggccggt tccggctccc
     3361 ccacctgccg ccgcgcccgt tcgacctgct cgccagcgcc tccggcttcg gaccgctgcg
     3421 ggtccccggc ctcgtgccgc ccgcttccag aaacccggga ggcgcggtgc tggacctcgg
     3481 agacgtggtc cttcccccgt gcgtcgccat cgagggacgg gtgaccgacc gccgggggag
     3541 ccctctggcg gaggcgaatg tagggctcgc ggagaccatc cggaatgccg agccgagccc
     3601 cggctcccag gtgaccactg gaccggacgg ccggttccgg ctcgccgatc tgcggcaggg
     3661 cgagcgctac gatctctggg tacagcacca ggggcacgtg tccgcgcacg tccccggcgt
     3721 cgaggcaccg acggacgagc cgttgcgcgt cgagctccgg ccggcccgcg tcctcgccgg
     3781 ccgggtggtc ggccctgccg gcgagccggt cccctacgcg tcgctgctgg atttcgagga
     3841 gaacggcgcc ggccaggggg acgggctctc ctcctacggc tcccaggcga gcaccctgac
     3901 ccggaccgac gcccagggcc gcttccgcgc cgacggtgtg gagcccggga ccctgaacct
     3961 ggaggtcaag gctccggaat acaagacccg ccggctgcgg ggcttggtca ttcccgagga
     4021 gtcggacgtc gagaacctgg agatcgtcct ggaacgggga gccacggtca ccgggcgggt
     4081 cctcgacgcc cgcggcgagc ccgtggcggg tgccgatgtg agcgccacac cgatggaggc
     4141 cggcaggggt atcggccggg aggaccactc cgcctcgtcg gagaccgacg gcgaagggcg
     4201 ctaccgtttg ggcgggctcg acccgggcag ccaccggatc acggcgaacc ggtacgccga
     4261 gggccagcac acagagacgg agatcctcgt ccggcccgga gaaaacgtcg tgaatctcgc
     4321 cttcaggcgt atcacggagg tctccggccg ggtggtggac gagagcggcg agcccatctc
     4381 caacgcctcc gtatccttgg gcggtgccca cgacgccaag ggggtgcgta gcggcccggg
     4441 cggtctgttc cacttcccgg cggtcgagga tggagactac cagttgagcg gcggtggctc
     4501 gggcttcatt ggccccgtcg agccggtggc ggtggaggtc gacggccggc cggtgcgcga
     4561 cctcgagctc cgtctgcgcc ggggtgggac cctgctgacc ggccggctcc tcggcttggc
     4621 gccgggcgag ttggagcgag tgaccgtcgc cgcccaggcc gaaggcaccc cctggccgcg
     4681 cccgggcacg gtgagccgcg acggcaccta tcgccttccc gatcttgagc ccggcacctg
     4741 ggaggtcacc gccgcgctga gctcggggcg ccgggcccgc gcctcgatcc gactggagcc
     4801 gggtcaggag cgggcggtgc tcgacctgga gatcacctcc ggcctggccc tcgccggccg
     4861 cgtgctggcg gacggcgcgc cccttcccgg tgtccggatt ctgctgagcc gccaacgcgg
     4921 ccgcggcgcc gagaccacga ccgtccacga cggccggttc cggctcgaag ggctggagcc
     4981 gggcgcctat gtcctgctgg cgacccactc cgccaccggc ctgggcaccg ctcaggcggt
     5041 cgatctctcc caggaccgtg acctgctggt ggagattgcc tccggcgcgg tccggggcca
     5101 ggtggagctg cccccgggag agccggcagc cggtctcgac atcttcctgg aagggaccga
     5161 ccctgccctc ggcgtctcct tcctgggccc ctccgtccag accgacgctc aggggagctt
     5221 cgagcttccc cgggtcgccg ccggacgcta ccgtctgacc gtccgccgcc gggggagccc
     5281 gctggcgacg gccgaggtcc gggtcgcgcc cggggaggcg gcgacggtcc gggtggagcc
     5341 gggcgcgggc ggcgtccact agatcggggg cgatttctcc gggcctcgac ccgactcatc
     5401 gggcgttcga cccgactcat cggacgttgg acccgagggt cgaacggttc gaccctcgtc
     5461 cgacctctcg cgacccgagg gtcgcgcctg tggggagtcg ggtcgcgcct atggtgggtc
     5521 gggtcgagcc tcctcgcagc cgggtcgagc ctccctcaac gggagcagca cgcacgcggc
     5581 ccggctagac taccccgcgt gaccgtcacc gccggccagc tcctcgccga ggcccgaccg
     5641 cgcctcgcag ccaccccgtt cggcgccccg ccgcgcgagg cggcgttc
//
LOCUS       DASPJI010000762         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_562479, whole genome shotgun sequence.
ACCESSION   DASPJI010000762 DASPJI010000000
VERSION     DASPJI010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562479"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1443
                     /locus_tag="VEL74_21385"
     CDS             190..1443
                     /locus_tag="VEL74_21385"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HYG65148.1"
                     /translation="MQAMLDVQSPSASASDPGPAPAQRAERVGFWLFASHLFGIFGIA
                     LSNILLAVSVLAAPRTVRRPLELRELAPLLVPLGLYVLLLAAAIAASYQPRDSLRGLT
                     EVFNLSTLLLAPLLVRGEKDVRRLVDGLLMIAALSAAYGLGQYLFGYGDLDRRIRGTF
                     SHYMTFAGFLLICDLLLLASMTFSDRWRQPWRWVALAAVNVALVGSYTRGAWVALGFA
                     VTALLIVRAPRLLLAYVPAAVLFILLAPVPLLQRVGSIADLRDSSNYDRLCMASAGLT
                     MIQERPLFGIGPEGVKHRYAIYRPATAPRYQVPHLHNSFLNLAAERGLPALASYLALT
                     LVSAGLAWRRYVREGGRHGPRADLWMGVLLALFAFNVAGLFENNWGDTEVQRPLLFVL
                     ALPFCLLAGGREREEAEAGEAPVIH"
     gene            1517..2248
                     /locus_tag="VEL74_21390"
     CDS             1517..2248
                     /locus_tag="VEL74_21390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HYG65149.1"
                     /translation="MRLDQLLVQRGLFGSREQARRAVMAGTVEVDGRRVDKPGTPVAE
                     DARLELQAPKEPFVSRAGRKLAHALDHFAIDPAGRVCLDVGASTGGFTDCLLQRGAAR
                     VYALDVGYGQLDHRLRQDPRVVVMERVNARHLEPDALPEPVDLITVDVSFISLTKVVP
                     ALVPHLASGGLLLPMIKPQFEAGRGMVGKGGILRDETVRARVVEQCAGEMAAQGLALL
                     GVADSAVPGVGGNREAFALLRKEAP"
     gene            2245..3192
                     /locus_tag="VEL74_21395"
     CDS             2245..3192
                     /locus_tag="VEL74_21395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HYG65150.1"
                     /translation="MTVPTVPDTTPPKKPAKARAGRGRRRLERVGIVAKAASQEAVHT
                     AHELAEWLRRRGLEAALDEATLRARGTSSEAPFRPEEPHDLVVALGGDGTLLAAARTL
                     APDVPILGVNLGNLGFLTEVGRAELYPAMVQVLSGKFETEERSLLDVELLRNGSGSLS
                     FRVFNDAVITKSALARIIELTFSFDGHVIARCRSDGLIISTPTGSTAYNLSAGGPILY
                     PLLPVIILTPICPHTLSLRPVVVPDAGRVEITLETQREEVYLTLDGQEGTSLGYRDTV
                     RVTRSAASVRLVKVSGRSFYDSLRGKLRWGGLERGGGPE"
     gene            3189..>4027
                     /locus_tag="VEL74_21400"
     CDS             3189..>4027
                     /locus_tag="VEL74_21400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65151.1"
                     /translation="MSEPEEPSSPPDAPAGGERRRGDRRRRGSGAWEGPERRAAERRR
                     PGRVRRWVVRPFVWGLLIVALVLLGALLFIESRYAHQRAADLVVARLGDVLNRKIEVG
                     GVDYGFAPLSFELRDLVIAGPRPEDPPFAVVPFVRFQFSWRDLRQRVLRLEQIEAIGP
                     RIYVRFNPDGTSNLPQLRRQPQRTRRFEVQVGRILVEGGSVQLDEKRLPLDIEARGVW
                     GRLIGRAERQGEGGNRFDAWLTVPEIRTTLPNARPYPYEILAAKGSFEPGRIRIASAL
                     MSGP"
BASE COUNT          484 a         1529 c         1409 g          605 t
ORIGIN      
        1 ccggcggaga tccgcggcac gctgctcgag aagtaccgcg agcacttccg ggacgaggag
       61 ccggtggtcg agcaggggtt gtactgcgcc gcctccgggc tggtctatcc catcgtctcg
      121 gacatcccga tcatgctgat cgaggaggcc ctccccgcct cggccgtcgg ccgctgagcc
      181 tgccggctca tgcaggcgat gctggacgtg cagtcaccat ccgcatcggc atccgacccg
      241 ggcccggccc cggcgcagcg ggccgaacgg gtaggctttt ggctcttcgc ctcgcatctg
      301 ttcggtatct tcggcatcgc cctgtcgaac atcctgctcg ccgtctcggt cctggcggcg
      361 ccgcggacgg tgcgccgccc cctcgagctc cgggagctgg cgccgctctt ggttcccctc
      421 ggcctctacg tcctgttgct cgccgccgcc atcgccgcct cctaccagcc gcgggacagc
      481 ctgcgcggcc tgaccgaggt gttcaacctc tcgacgctgc tcctcgcccc cctcctggtg
      541 cgcggcgaga aggacgtccg gcggctggtc gacggcctgc tgatgatcgc cgcgctctcc
      601 gccgcctacg ggctcggcca gtacctgttc ggctacggcg acctcgaccg ccggatccgc
      661 ggcacgttct cccactacat gacgttcgcg ggcttcctgc tgatctgcga cctgctgctc
      721 ctcgcctcga tgaccttttc ggaccgctgg cgccagccct ggcgttgggt ggccctggcc
      781 gcggtcaacg tcgccctggt ggggagctac acccgcggcg cctgggtggc gctgggcttc
      841 gcagtgaccg ccctcctgat cgtccgcgcg ccgcggctcc tcctggccta cgtgccggcg
      901 gccgtgctgt tcatcctgct ggcgccggtg ccgctgctgc agcgcgtcgg ctccatcgcc
      961 gacctgcggg actcgtccaa ctacgaccgc ctgtgcatgg cgagcgccgg cctgaccatg
     1021 atccaggagc ggccgctgtt cggcatcggg cccgaggggg tcaagcaccg ctacgccatc
     1081 taccggccgg cgacggcgcc gcgctaccag gtgccgcatc tgcacaacag cttcctgaac
     1141 ctcgcggcgg agcgggggct gccggccctc gcctcctatc tggcgctgac cctggtgagc
     1201 gccggcctcg cctggcgccg ctatgtccgc gagggaggac gccacggccc ccgtgccgac
     1261 ctctggatgg gagtgctgct ggcgctcttc gccttcaatg tcgccggcct gttcgagaac
     1321 aactgggggg acacggaggt gcagcgcccc ctgctgttcg ttctcgccct ccccttctgt
     1381 ctgctcgccg gcggccggga gcgggaggag gcggaggccg gcgaggcccc ggtgatacac
     1441 tgacgagccc cgggtacgcc cgttgcatgg ctcgcgggcg cccaatgacc gagacagggc
     1501 acggggatcc accgcgttgc gccttgacca gctactcgtc cagcgaggcc tcttcggcag
     1561 ccgggagcag gcgcgccgcg ccgtcatggc cgggaccgtc gaggtcgacg gccggcgggt
     1621 cgacaagccg ggcacgccgg tggccgagga cgcccgcctc gagctccagg cgccgaagga
     1681 gcccttcgtc tcccgcgccg gccgcaagct ggcccatgcc ctcgaccact tcgccatcga
     1741 tccggccggc cgcgtctgcc tcgacgtcgg cgcctcgacc ggcggcttca ccgactgtct
     1801 gctgcaacgg ggggcggcac gggtctacgc cctggacgtg ggctacggcc agctcgacca
     1861 ccggctgcgc caggacccgc gggtggtggt gatggagcgc gtcaacgccc gccacctgga
     1921 gccggacgcc ctgccggagc cggtcgacct gatcaccgtc gacgtctcct tcatctccct
     1981 caccaaagtg gtgccggcgc tcgtgccgca cctcgcctcc ggcggcctgc tgctgccgat
     2041 gatcaagccg cagttcgagg ccggccgcgg catggtgggc aaggggggca tcctgcgcga
     2101 cgagacggtg cgcgcccggg tggtcgagca gtgcgccggc gagatggcgg cgcaggggct
     2161 ggccctcctg ggggtcgccg actccgccgt gcccggcgtc ggcggcaacc gcgaggcgtt
     2221 cgcgctgctg cgcaaggagg cgccttgacc gttccgaccg ttccggacac gacgcccccg
     2281 aagaagccgg ccaaggcgag ggcgggccgc ggccggcgcc gcctggagcg cgtcggcatc
     2341 gtcgccaagg cggcgagcca ggaggcggtc cacaccgccc acgagctggc cgagtggctg
     2401 cgccgccgcg gcctcgaagc cgccctcgac gaggccaccc tgcgcgcccg cggcacctcc
     2461 agcgaagccc ccttccggcc cgaggagccc cacgacctgg tggtggcgct gggcggcgac
     2521 ggcaccctgc tcgccgccgc ccgcaccctg gcgccggatg tccccatcct gggggtgaac
     2581 ctggggaacc tcggcttcct caccgaggtc ggccgtgccg agctctaccc ggcgatggtc
     2641 caggtgctgt ccggcaagtt cgagaccgag gagcggtcgc tgctcgacgt cgagctgctg
     2701 cgcaacggca gcggctcgct ctccttcagg gtgttcaacg acgcggtcat caccaagagc
     2761 gccctcgccc gcatcatcga gctgaccttc tctttcgacg gccacgtcat cgcccgctgc
     2821 cgctccgacg gcctgatcat ctccaccccc accggctcga ccgcctacaa cctgtcggcc
     2881 ggcgggccga tcctctaccc gctgctgccg gtgatcatcc tcaccccgat ctgcccgcac
     2941 accctgtcgt tacggccggt ggtggtgccg gacgccgggc gggtggagat caccctggag
     3001 acgcagcgcg aggaggtcta cctcaccctc gacggccagg aggggacctc cctcggctac
     3061 cgggacaccg tgcgcgtcac ccgcagcgcg gcgtcggtgc ggctggtcaa ggtgagcggc
     3121 cgcagcttct atgacagcct gcgcgggaag ctccgctggg gcggcctgga gcggggaggg
     3181 ggaccggagt gagcgagccg gaggagccct catctcctcc tgacgcgccg gcgggtggcg
     3241 agcggcggcg tggcgaccgg cggcggcgcg gctccggagc ctgggagggt cccgagcggc
     3301 gcgccgccga gcggcggcgt ccgggccggg tgcggcgctg ggtggtccgg ccgttcgtct
     3361 ggggcctgct catcgtcgcc ctggtgctcc tcggcgcgct gctgttcatc gagagccgct
     3421 atgcccacca gcgcgccgcc gacctggtgg tggcgcgcct cggcgacgtg ctcaaccgca
     3481 agatcgaggt gggcggggtg gactacggct tcgcccccct ctccttcgag ctgcgcgacc
     3541 tggtcatcgc cggcccgcgg cccgaggatc cccccttcgc cgtggtgccg ttcgtccgct
     3601 tccagttctc ctggcgcgac ctgcgccagc gggtgctgcg cttggagcag atcgaggcca
     3661 tcggcccgcg catctacgtg cgcttcaacc cggacggcac cagcaacctg ccgcagctcc
     3721 gccgccagcc gcagcggacc cgccgcttcg aggtgcaggt cgggcgcatc ctggtggagg
     3781 gggggagcgt gcagctcgac gagaagcggc tgccgctcga catcgaggcc cgcggcgtct
     3841 gggggcgcct catcggccgc gcggagcgcc agggcgaggg ggggaaccgc ttcgacgcct
     3901 ggctcaccgt gccggagatc cgcaccaccc tgcccaacgc ccgcccctac ccctacgaga
     3961 tcctggcggc caaggggagc ttcgagcccg gccgcatccg catcgccagc gccctgatga
     4021 gcggccc
//
LOCUS       DASPJI010000763         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_726286, whole genome shotgun sequence.
ACCESSION   DASPJI010000763 DASPJI010000000
VERSION     DASPJI010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726286"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2399
                     /locus_tag="VEL74_21405"
     CDS             <1..2399
                     /locus_tag="VEL74_21405"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HYG65152.1"
                     /translation="KVIYHLLGGLHSTETGSPEMLMELAYRLAVSERPEIRAIRENAI
                     VLITPVVEPDGRDRMVDWYERNLRGRDLPYEVLTQIDSPPYWGRYVFHDNNRDGMQLT
                     QALTRAVHETFYAYHPQVMHDLHESVPLLYISTGTGPYNPAIDPVTINEWTQIAHHEA
                     GALAGMGLPGVWVWGFWDGWWPGYLNSVANNHHGISRFYETFGNTLPGTYERDLSESR
                     YAGKKVTEVQWYRPWPPDKKVRWSLRNNTNYMQAGVLAALHYAALHRRELLEGVWLKA
                     SRSLEKGRSQAPYAWVFPAEQRDPARLAHLVNQLRAHRIEVHRLTAELKLGSKSWPAG
                     SYVVRMDQPYRNLAVTLLEEQKFPADEPNPPYDDVAWTWPLLYGVAGDRVDDKKVFDA
                     TPERMEAVTADVSAAGGIDGAATDGEVFLLRDTGQTSLLEARVLLGSHQVDAAETSFK
                     AGGVSYPAGSWIVQAPRGEVEEIAARLGLRFAAAGAMPDVRRHLVDLPRLALLHTWTA
                     TQDAGWARYTLDRAGVPYTLISDGDLRRGGLVERFDVILFPNSRGDFASMVHGIDPAW
                     GPLAYTSTPETPSLGTPNAAEDITGGMGFEGLVELQRFLRGGGTLITLANSGTLAVQG
                     GLVRRIEGPERPFTPGSELTAKVVRPEHPLAYGYEEITSVFRGNGPLWDVDKRDRGRV
                     VLQFGTKDVDKEEEKDKDASGTIEVEDIDDTEPGTKKDEKKEKEAPKKKLVLSGFVRN
                     ESEVDGKPAILDLPVDGAGGESGGRVILFAFNPLHRHLNLSDFRLVYNAILHWNDLPR
                     "
     gene            2484..>2741
                     /locus_tag="VEL74_21410"
     CDS             2484..>2741
                     /locus_tag="VEL74_21410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65153.1"
                     /translation="MKPSLHKTPERQPAGNHAGLPSEAAAPGAGPRRVSPSNPASVVE
                     RWALRLAAAAIARPRLAVILALVVVLAAAPGLLRLELRTDGR"
BASE COUNT          444 a         1013 c          900 g          384 t
ORIGIN      
        1 gaaaagtgat ctaccacctc ctcggcggcc tccactcgac cgagaccggc agccccgaga
       61 tgctgatgga gctagcctac cgactcgccg tctccgagcg gcccgagatc cgcgccatcc
      121 gcgagaacgc catcgtcctc atcacccccg tggtggagcc cgacggccgc gaccgcatgg
      181 tggactggta cgagcgcaac ctgcggggcc gggaccttcc ctacgaggtg ctgacccaga
      241 tcgacagccc gccgtactgg ggacgctacg tcttccacga caacaaccgc gacggcatgc
      301 agctcaccca ggccctcacc cgcgccgtcc acgagacctt ctacgcctat cacccgcagg
      361 tcatgcacga cctgcacgag tcggtgccgc tcctctacat ctccaccggg accgggccct
      421 acaacccggc catcgacccg gtgacgatca acgagtggac ccagatcgcc caccacgagg
      481 ccggagcgct ggccggcatg gggctgcccg gcgtctgggt ctggggcttc tgggacggct
      541 ggtggcccgg ctatctcaac tccgtggcca acaaccacca cgggatttcc cgcttctacg
      601 agaccttcgg caacaccctg cccgggacct acgagcgcga cctctccgaa tcccgctacg
      661 ccggcaagaa ggtcaccgag gtccagtggt accgcccctg gccgccggac aagaaggtcc
      721 gctggtcgct gcgcaacaac accaactaca tgcaggccgg agtgctcgcc gccctccact
      781 acgccgccct gcaccgccgg gagctcctgg agggggtctg gctcaaggcg agccggtcgc
      841 tggagaaggg gaggagccag gccccgtacg cctgggtctt cccggccgag cagcgcgacc
      901 ccgcccgcct cgcccatctg gtcaaccagc tccgcgctca ccggatcgag gtccaccgcc
      961 tgaccgccga gctgaagctg gggagcaaga gctggcccgc cggcagctac gtggtccgca
     1021 tggaccagcc gtaccgtaac ctcgccgtca ccctgctcga ggaacagaag ttccccgccg
     1081 acgagcccaa cccaccctat gacgacgtgg cctggacctg gcccctcctc tacggcgtgg
     1141 ccggcgaccg ggtggatgac aagaaggtct tcgatgctac cccggagcga atggaggcgg
     1201 tgaccgccga cgtctccgcc gccggtggca tcgacggggc ggcgaccgat ggggaagtct
     1261 tcctgctccg cgacacgggc cagacgtcgc tcctcgaggc gcgcgtcctc ctggggagcc
     1321 atcaggtgga cgcggccgag acctccttca aggccggcgg cgtctcgtat ccggcgggga
     1381 gctggatcgt ccaggcgccg cgcggcgagg tggaggagat cgcggcccgc ctcggcctgc
     1441 gtttcgccgc ggccggggcg atgccggatg tccgccgcca cctggtggac ctgccgcgcc
     1501 tggcgctgct ccatacctgg accgccaccc aggacgccgg ctgggcgcgc tacaccctgg
     1561 accgcgccgg cgtcccctac accctgatca gcgacggcga cctgcggcgc ggcggcctcg
     1621 tcgagcgctt cgacgtcatc ctcttcccga acagccgggg ggacttcgcc agcatggtgc
     1681 acggcatcga ccccgcctgg ggtcccctgg cctacaccag cacgcccgag acccccagcc
     1741 tcggcacgcc caacgccgcc gaggacatca ccggcggcat ggggttcgag gggctggtgg
     1801 agctgcagcg cttcctgcgc ggcggcggca ccctgatcac cctggccaac tccggcaccc
     1861 tggcggtgca ggggggcctg gtgcgccgga tcgaggggcc cgagcggccg ttcacgccgg
     1921 gctcggagct gaccgccaag gtggtgcggc cggagcaccc cctggcctac ggctacgagg
     1981 agatcaccag cgtcttccgc ggcaacggcc cgctctggga cgttgacaag cgcgaccgcg
     2041 gccgggtggt cctgcagttc ggcaccaagg atgtcgacaa ggaagaggag aaggacaagg
     2101 acgcgagcgg gacgatcgag gtggaggaca tcgacgacac ggagccgggc acgaagaagg
     2161 acgagaaaaa ggagaaggag gcgccgaaga agaagctcgt cctctccggc ttcgtccgca
     2221 acgagtccga ggtggacggc aagccggcaa tcctcgacct gccggtggac ggggccggcg
     2281 gcgagagcgg gggccgggtg atcctcttcg ccttcaaccc cctgcaccgc catctgaacc
     2341 tgtccgactt ccggctggtc tacaacgcca tccttcactg gaacgacctg ccccggtagg
     2401 gagagtgacg attttgtgag cttcgtgccg gcagggtgct cgctttcaag cccatggtgc
     2461 accatgtctc ttgaggacga cttgtgaagc catctctcca caagacgccc gagcgccagc
     2521 cggcgggcaa tcacgccgga ctcccttccg aagccgcggc cccgggcgcg gggccgcggc
     2581 gggtttctcc ctccaatccc gcgagcgtcg tcgagcgctg ggctttgcgg ctcgccgcag
     2641 ctgccatcgc ccgtccccgg ctggccgtca tcctggccct ggtcgtcgtc ctggctgccg
     2701 ctcccgggct cctccgtctg gagctgcgca ccgacggccg c
//
LOCUS       DASPJI010000764         5355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_49439, whole genome shotgun sequence.
ACCESSION   DASPJI010000764 DASPJI010000000
VERSION     DASPJI010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49439"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2457
                     /locus_tag="VEL74_21415"
     CDS             <1..2457
                     /locus_tag="VEL74_21415"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HYG65154.1"
                     /translation="AFLLMELVWYRMLAPLLGGSSYTFGLILAVALLGIGAGGLLYGA
                     GPLKRRPTLLAFAVTCALEALFLALPFALGDRVAVLAALLRPLSGAGFGPLVLAWTAI
                     TSLVVLPAALVAGYQFPLLIALLGSGRKEVGREVGLTYAANTVGAILGSIAGGFGLIP
                     LLSAPGTWRLVVLLLLALAGVAAAAALRSGSPLRAAAAPVAVGAVALVCCFMPGPTAF
                     WRHSPIGAGRVRAADWQGPNDLRSTMHSRRRGLIWERDGVESSVALEGKQELAFLVNG
                     KADGSARTDGPTQVMSGLIGAILHPDPRRSLVIGLGTGSTAGWLAAVPGMERVDVVEL
                     EPAIEDVARACAPVNHQALDDPKLHLVIGDGREVLLTTGERYDVIFSEPSNPYRAGIA
                     SLFTADFYEAARQRLRPGGLFIQWLQGYELDAQVVRTVYATLAAVFPSVETWEVNAGD
                     LLLVASLEPMAHDVGRVRARAAQEPYRSALSRTWGVGGAEGLYTGYLASPAFARAVAQ
                     GEGGGVNTDDRPVLEFGFARNLGRSGLFRTEDLVRLARDRREDRLTVGRGSLDVALLE
                     ELRTARSAFWERTPDDPPADAGVDFGSRVRARQAYLTPSMTQSCAHWLAQPEPPRADI
                     DVLLLAECLASRGDARALEQVPRLRQRSPTEAELVLALYHGSAGNRDLARHHLTTAIR
                     AFRTDPWPFLPMAERILFYEVRRGQGDPETARILFSELEQPFAVNLLNEGRQQLRVDL
                     SRVLGRSEDCIGALAPFEPDVPWEEGFLRQRYRCYRLANHRLEGRAGRDLEAFLDAAP
                     PSLEAGLPAALPSPAPSPAP"
     gene            2529..3389
                     /locus_tag="VEL74_21420"
     CDS             2529..3389
                     /locus_tag="VEL74_21420"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013877678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HYG65155.1"
                     /translation="MESIRTIGVVGAGTMGHGIAQVAAHSGFDVILVDVAPQALERGV
                     AQIGKGLERLVAKGKLAAEERDQALARVRPESRVAALAAADLVVEAVVERLEVKQAVL
                     AELDRACRPAAILATNTSSISITKLAAATSRPEKVIGMHFMNPVPVMQLVEIIRGLAT
                     SQETYGLVEAASRAMGKTPVEVHDAPGFVSNRVLMPMINEAIFCLYEGVGKPEAIDSV
                     MKMGMNHPMGPLALADLIGLDVCLDILRVLHEGFGDPKYRPCPLLVKMVDAGYLGRKS
                     GRGFYDYAGS"
     gene            3433..5280
                     /locus_tag="VEL74_21425"
     CDS             3433..5280
                     /locus_tag="VEL74_21425"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG65156.1"
                     /translation="MYCQVCGALNRDDHEFCVRCHQKLLVLSGVVREEEEPYEEGLEE
                     SFSFDEHLLERISILEEAVKRTAETVRQVLSALHKQEKHILINQTGLSTLRELLEQKK
                     VVGWEEWSDLWESKMDYQLLALEKRERFLAIKERIAALHHGDKRKVFLQHLEDAEYAL
                     FAFDIERAVTALESAFKLDRDNYELAYFLGETYFNEGAAEQALSYFVRVLEVKPDHYE
                     GLVYSGVIHYERGDHNRAEEYLKRAVSIYPESFLPHFSLGAVYAGQGNLAKAVLFLER
                     AVQVDAVPQALYLLGSCLYEMGRLSAAIENLQEAVRHDPAFEEAYHLLGLAYLDRHWT
                     RKALEAFRQAQRLNPKRLRYQDLIRYLSGHDGTPLPGVDGEAGVWFTQGEDSLARGNL
                     KQALFSYRRALALDPENPTLLMSYALLCLHLNRSQEIEAVTRKVLDLNPGETLKATAY
                     AALMEALRSQGKFREGNRIGLLLLDEGSSNFTKSIAYYEMAYNLAEMEEDLDKALDYA
                     RQSVELSPDEIKQFPLAALGWVHYKRKEFDKAIDFLSKSSDLGPSSNTLTHLGMALLA
                     SGEEEEARSVLARARTLGARGEGLQEKMMECMKDSTRLLQRVRKSAPKK"
BASE COUNT          746 a         1980 c         1838 g          791 t
ORIGIN      
        1 gccttcctcc tcatggagct ggtctggtac cggatgctcg caccgctcct cggcggctcg
       61 tcctacacgt tcggcctgat cctcgccgtc gccctcctgg gtatcggcgc cggcggcctc
      121 ctctacggcg ccgggccgct caagcgccgg ccgacgctcc tcgccttcgc cgtcacctgc
      181 gcgctggagg cgctcttcct cgccctcccc ttcgccctgg gggaccgcgt cgccgtgctc
      241 gccgccctgc tgcgccccct ctccggcgcc gggttcggcc cgctggtcct ggcctggacg
      301 gcgatcacct ccctggtggt gctgccggcg gcgctcgtcg ccggctatca gttccccctg
      361 ctcatcgccc tcctggggag cggacggaag gaggtggggc gcgaggtggg gctcacctac
      421 gccgccaaca ccgtgggcgc gatcctcggc tccatcgctg gcggcttcgg cctgatcccc
      481 ctgctctcgg cgccggggac ctggcggctg gtggtgctcc tcctcctcgc cctcgccggc
      541 gtggcggccg cggccgccct gcgatccggc tcgccgctcc gagccgccgc ggccccggtg
      601 gcggtgggcg ccgtcgccct ggtctgctgc ttcatgccgg gccccaccgc cttctggcgc
      661 cacagcccca tcggcgccgg acgcgtccgc gccgccgact ggcaggggcc caacgacctg
      721 cggagcacca tgcactcccg ccgccgcggc ctgatctggg agcgggacgg ggtcgagagc
      781 agcgtcgccc tggaggggaa acaggagctc gccttcctgg tcaacggcaa ggccgacggc
      841 agcgcccgca ccgacggacc cacccaggtg atgagcggcc tgatcggggc catcctccat
      901 ccggacccgc ggcgctccct ggtcatcggc ctggggaccg gcagcaccgc cggctggctg
      961 gccgccgtcc cgggtatgga gcgggtcgac gtggtggagc tggagcccgc catcgaggac
     1021 gtggcccgcg cctgcgcgcc ggtgaaccac caggccctgg acgaccccaa gctccacctg
     1081 gttatcggcg acggccggga ggtcctcctc accaccggcg agcgctacga cgtcatcttc
     1141 tccgagccct ccaaccccta ccgcgccggc atcgccagcc tgttcaccgc cgacttctac
     1201 gaggcggccc gccagcggct gcgccccggc ggcctgttca tccagtggct gcagggatac
     1261 gagctggacg cccaggtggt gcgcaccgtc tacgccaccc tggccgcggt cttcccgtcg
     1321 gtcgagactt gggaggtcaa cgccggcgac ctgctgctgg tggccagcct ggagccgatg
     1381 gcccacgacg tcggccgggt gcgcgcccgc gccgcccagg agccctatcg cagcgccctc
     1441 tcccggacct ggggtgtcgg cggcgccgag gggctctaca ccggctacct cgcctccccg
     1501 gcgttcgcgc gggcggtggc gcagggcgag gggggagggg tcaacaccga cgaccggccg
     1561 gtgctggagt tcggcttcgc ccgcaacctc gggcgctccg gcctgttccg cacggaggac
     1621 ctggtgcggc tggcgcggga ccggcgcgag gaccggctga cggtaggccg cgggtccctg
     1681 gacgtggccc tgctggagga gctgcgcacc gcccggtccg ccttctggga gcggaccccg
     1741 gacgatccgc ccgcggacgc cggcgtggac ttcgggtcgc gggtccgggc gcggcaggcc
     1801 tatctcaccc cctccatgac ccagtcctgc gcccactggc tcgcccaacc cgagccgccg
     1861 cgcgccgaca tcgacgtcct gctgctcgcc gagtgcctcg cctcccgggg agacgcccgg
     1921 gcgctggagc aggtgccccg gctgcggcag cgcagcccca ccgaggcgga gctggtgctg
     1981 gcgctgtacc atggctcggc ggggaaccgg gacctggccc ggcatcacct gaccacggcc
     2041 atccgcgcct tccgcaccga tccctggccc ttcctcccca tggccgagcg gatcctcttc
     2101 tacgaggtcc ggcggggtca gggggatccg gagaccgccc gcatcctctt cagcgagctg
     2161 gagcagccgt tcgccgtcaa cctcctgaac gaggggcggc agcagctccg cgtcgacctc
     2221 tcccgggtcc ttggccggtc ggaggactgc atcggcgccc tcgccccctt cgagcccgac
     2281 gtgccgtggg aggaggggtt cctgcgccag cgctaccggt gctaccggct ggcgaaccac
     2341 cggctggagg ggcgggcggg ccgtgacctg gaagctttcc tggatgccgc gccccccagc
     2401 ctggaggcgg ggctgcccgc cgcccttccc tcccccgccc cctcccccgc gccttgaccc
     2461 ccggcgcgcc atctctatag gctcacgggg ccacccgctt ccgggtggat acgggagggg
     2521 tttatagaat ggagtcgatc cgaaccatcg gagtcgtggg cgccgggacc atggggcacg
     2581 gcatcgccca ggtggcggcc cacagcggct ttgacgtcat cctggtggac gtcgcgccgc
     2641 aagcgctgga gcgcggcgtc gcgcagatcg gcaaggggct ggagcgcctg gtcgccaagg
     2701 ggaagctcgc cgccgaggag cgcgaccagg ccctcgcccg cgtccgcccc gagtcccggg
     2761 tcgccgccct cgcggccgcc gacctggtgg tcgaggccgt ggtcgagcgc ctcgaggtca
     2821 agcaggcggt gctcgccgag ctcgaccgcg cctgccggcc ggcggccatc ctcgccacca
     2881 acaccagctc catctcgatc accaagctgg ccgcggccac gtcgcggccg gagaaggtca
     2941 tcggcatgca cttcatgaac ccggtgccgg tgatgcagct cgtcgagatc atccgcggcc
     3001 tcgccacctc ccaggagacc tacggcctgg tcgaggcggc gtcccgcgcc atgggcaaga
     3061 ccccggtcga ggtgcacgac gcccccgggt tcgtctccaa ccgcgtgctc atgccgatga
     3121 tcaacgaggc catcttctgt ctctacgagg gggtggggaa gcccgaagcc atcgacagcg
     3181 ttatgaagat gggtatgaac cacccgatgg gccctctggc gctggccgac ctcatcggcc
     3241 tggatgtctg tctcgacatc ctgcgggtgc tccacgaggg tttcggcgac ccgaagtacc
     3301 gtccctgccc gctcctggtg aagatggtcg acgccggtta cctcggccgc aagtccggcc
     3361 ggggtttcta cgactatgcc gggagctgac gcagcggccg gcgcgggcag ggcccggccg
     3421 gcggaggatc ggatgtactg tcaagtctgt ggcgctctga accgggacga ccacgagttc
     3481 tgcgtccgct gccaccagaa gctgctggtg ctgtcgggcg tggtgcgcga ggaggaggag
     3541 ccgtacgagg aggggctgga ggagagcttc tccttcgacg agcacctcct cgagcggatc
     3601 tccatcctgg aggaggcggt caagcggacc gccgagacgg tgcgccaggt gctctccgcc
     3661 ctgcacaagc aggagaagca catcctcatc aaccagaccg ggctgtcgac gctgcgcgag
     3721 ctgctcgagc agaagaaggt cgtcggctgg gaggagtgga gcgacctctg ggagtccaag
     3781 atggactacc agctcctcgc cctggagaag cgggaacgct tcctcgccat caaggagcgg
     3841 atcgccgccc tgcatcacgg cgacaagcgc aaggtcttcc tccagcacct ggaggacgcc
     3901 gagtacgccc tcttcgcctt cgacatcgag cgcgcggtga ccgccctcga gtccgccttc
     3961 aagctcgacc gcgacaacta cgagctggcc tacttcctgg gcgagaccta tttcaacgag
     4021 ggggccgccg agcaggcgct gtcgtacttc gtccgcgtcc tcgaggtgaa gccagaccat
     4081 tacgaggggc tggtctacag cggcgtcatc cactacgagc ggggggacca caaccgcgcc
     4141 gaggagtacc tgaagcgcgc ggtgtcgatc tacccggaga gcttcctccc ccacttcagc
     4201 ctgggcgcgg tctacgccgg ccaggggaac ctggccaagg ccgtgctgtt cctcgagcgg
     4261 gcggtgcagg tggacgcggt gccgcaggcc ctgtacctcc tgggaagctg cctttacgag
     4321 atgggccgcc tgtcggcggc gatcgagaac ctgcaggagg cggtgcggca cgacccggcg
     4381 ttcgaggagg cctatcatct gctggggctc gcctatctcg accgccactg gacccgcaag
     4441 gcgctggagg cgttccgcca ggcgcagcgg ctgaacccca agcgcctgcg ctaccaggac
     4501 ctgatccgct acctctccgg ccacgacggc acgccgctgc ccggcgtcga cggcgaggcg
     4561 ggcgtctggt tcacccaggg cgaggactcg ctggcccgcg gcaacctcaa gcaggcgctg
     4621 ttctcctatc gccgcgccct ggcgctcgat ccggagaacc cgacactcct catgtcgtac
     4681 gccctcctct gcctgcacct gaaccgcagc caggagatcg aggcggtcac ccgcaaggtg
     4741 ctggacctga acccgggcga gacgctcaag gccacggcct acgcggcgct gatggaggcg
     4801 ctgcgcagcc agggcaagtt ccgcgagggc aaccgcatcg gcctcctgct cctcgacgag
     4861 gggagctcca acttcaccaa gtcgatcgcc tactacgaga tggcgtacaa cctggcggag
     4921 atggaagagg atctggacaa ggcgctggac tacgcccgcc agtcggtgga gctgtcgccc
     4981 gacgagatca agcagttccc gctggcggcg ctcggctggg tgcactacaa gcgcaaggag
     5041 ttcgacaagg ccatcgactt cctgtccaag tcgtccgacc tgggcccctc ctcgaacacg
     5101 ctgacccacc tgggtatggc gctcctcgcc tcgggcgagg aagaggaggc gcgctcggtc
     5161 ctcgcccgcg cccgcacgct gggcgcccgc ggcgaggggc tgcaggagaa gatgatggag
     5221 tgcatgaagg actcgacgcg cctcctccag cgggtccgca agagcgcgcc gaagaagtag
     5281 gggggcctcc gcgcccatgc cgcccctcct gcgcatcgac cctggcgatc cccgcccgat
     5341 ctggcggcag atcga
//
LOCUS       DASPJI010000765         3601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_220167, whole genome shotgun sequence.
ACCESSION   DASPJI010000765 DASPJI010000000
VERSION     DASPJI010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220167"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1511
                     /locus_tag="VEL74_21430"
     CDS             240..1511
                     /locus_tag="VEL74_21430"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HYG65157.1"
                     /translation="MTPTSARINAKLVRESARVRLQVSPALRIPGLEEPLRTPFQTAT
                     PARANVVSFEVTGLRPDTEYRYAVEVDGEIEEDRQGRFRTFPDRPASFRVVLASCANT
                     GSDHPVFDTIRGLDPLFFLHMGDFHYEDISRPERRLYRRAFDAVFASPRQSVLYRDVP
                     VAYMWDDHDFGGNNSDRTSRGREAARLTYQEYVPHYPLPAGRGDVPIYQAFTAGRVRF
                     IVTDLRSARSPAGSADDAGKTMMGAAQKAWFLRELLDAHRAGMLVAWVSTDPWIEQKT
                     QGSDRWGGYSTERREIADFIARHRIDGRLVVLSGDAHMLAMDDGTHSGYSSAGGRGFP
                     VLHAAALDRRGSVKGGPYTEGPFPNRTRITRHDGQFAVMEVRDEGGPEICIEWSGRRQ
                     QLRTPIVTELFHWERCFPAPPAPAPASPGPL"
     gene            1577..>3601
                     /locus_tag="VEL74_21435"
     CDS             1577..>3601
                     /locus_tag="VEL74_21435"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG65158.1"
                     /translation="MRSPKSLLVGCLAAALAGGAGPAPEVRDRRPGEAVERELAGGEA
                     HAWRLETAAGRPLLVTVEQRGTDVTIEAFSPQGGSVAVVDTPTGRDGPESLLVEPAAA
                     GSWRLEVRSPARAAAPGRYAIRVEELDPVSPTGQRRIKAERLMTDAARLYGAGTADAR
                     RQAAERYTAALALWSELGHREKEAAALFCLGALHRSLGDARKALDAYEPALPLWRGLG
                     NREREIDTLNRLGLSHSALGRQNEALALFRQALDIARDAVDPYREAEALANVGFIQHS
                     RGSLREALESYGRALERFRAAGDPWQEATVLKNLGGAWDRLGEPQQSLDHYRQALDLA
                     RALGDRAGEAGILANLAVTYRETGEMGEALARYDEALTLFRELGDRRWQGRVLNNIGF
                     AYLLLGEAGRAVPYYEQALELRRATGDRLGEAITLNNLGRARGVLGETRQALDLHRQA
                     LDLARDLGDRQEEANALGLLGQARAAAGDLAGALQDLGRAVELQRTVEDRRPLARFLQ
                     RTGEVQARQGDLPAALASFEQALALRRAMADRSGAAETLDSLARAERAAGRLDTARAR
                     AEEALGVIESLRGAVENPDLRASFLASQRRAYELYVDLLMELDGREPGRGWARAALEA
                     SERSRARSLLELLHEAGADVREGIDPGLRERERSLRQRLNAKALRRLELLS"
BASE COUNT          474 a         1358 c         1309 g          460 t
ORIGIN      
        1 ggtcaacggc tcgctgcgcg acgacgacgg cgcctcctcc tcggccgagc ggccgaagga
       61 ggtgcaggtg gtgctgcact gagccgcggc accacggcgg gggccgcggg caggtgatac
      121 cgttggcccc catgcggcgg ctcgtccttc ttctcgtcct tctcctcccc gccgccgggt
      181 gtcacagggc cgactcggcg cctccatccg gcccggtcca gttcgcctgg tccggagcgg
      241 tgacaccgac atcggcacgg atcaacgcca agctcgtgcg cgagagcgcc cgggtgcgac
      301 tgcaggtgag ccccgctctc cggattcccg gcctggaaga gcccctccgc accccgttcc
      361 agaccgccac cccggcccgc gccaacgtgg tctcgttcga ggtcacgggc ctgcggccgg
      421 acaccgagta ccgctacgcc gtcgaggtgg acggcgagat cgaggaggac cggcaggggc
      481 gcttccgcac ctttccggac cggccggcct ccttccgggt ggtgctcgcc tcttgtgcga
      541 acaccggctc cgatcatccg gtgttcgaca ccatccgcgg gctcgatccc ctgttcttcc
      601 tccacatggg ggacttccac tacgaggaca tctcgcggcc ggagcggcgg ctttatcgcc
      661 gcgccttcga cgcggtcttc gcttcgccgc gccagtcggt cctctaccgc gacgtgcccg
      721 tggcctacat gtgggacgac cacgatttcg gcggcaacaa ctccgaccgc accagccggg
      781 ggcgggaggc ggcgcggctc acgtatcaag agtacgtccc ccactacccg ctgccggcgg
      841 gccgcggcga cgtccccatc taccaggcct tcactgccgg ccgcgtccgc ttcatcgtga
      901 ccgacctgcg ctcggcgcgg agtcccgcgg ggtcggccga tgacgccggc aagaccatga
      961 tgggggcggc ccagaaggcc tggttcctgc gcgagctgct ggacgcccac cgcgccggca
     1021 tgctggtggc ctgggtgagc accgacccct ggatcgagca gaagacgcag gggagcgacc
     1081 gctggggggg ctatagcacc gagcggcgcg agatcgccga cttcatcgcc cggcaccgca
     1141 tcgacggccg cctggtggtc ctctcgggcg acgcccacat gctggccatg gacgacggca
     1201 cccactcggg ctactcctcg gccggggggc gcggcttccc cgtgctgcac gccgccgctt
     1261 tggaccgccg gggctcggtc aagggcggcc cctacaccga ggggcccttc ccgaaccgca
     1321 cgcgcatcac ccgccacgac ggccagttcg cggtcatgga ggtgcgcgac gagggcggcc
     1381 ccgagatctg tatcgaatgg agcggccgcc ggcagcagct ccgcaccccc atcgtgaccg
     1441 agctgttcca ctgggagagg tgctttcccg cgccgccggc gccggccccc gcctcccccg
     1501 gccccctcta gcggcgaatc ccgtctccgt atccacccga gcggtggtaa cctccactcg
     1561 tctcggcaaa aagacgatga ggagccctaa atcgctgctc gtggggtgcc tggccgcggc
     1621 tctggcaggc ggagccggtc cggcgcccga ggttcgggat cggcggccag gagaggcggt
     1681 cgagagggag ctggccggcg gcgaagccca tgcctggagg ctggaaacgg cggccggacg
     1741 ccccctgctg gtgaccgtcg agcagcgggg caccgacgtg accatcgagg ccttctctcc
     1801 ccaaggggga agcgtggccg tggtggacac cccgaccggg cgtgacggcc ccgagtccct
     1861 gctcgtcgag cccgcggcgg cggggagctg gcggctggag gtccgctcgc cggcgcgggc
     1921 ggcggcgccc ggccgctacg cgatccgcgt cgaggagctg gaccccgtgt ccccgaccgg
     1981 ccagcggcgc atcaaggccg agcggctgat gacggacgcg gcgcggctct atggcgcggg
     2041 gaccgcggac gcccgccggc aggcggcgga gcgctacacc gccgcgctcg ccctctggag
     2101 cgagctgggc cacagggaga aggaggccgc cgccctcttc tgcctgggcg ccctgcaccg
     2161 ctcgctgggc gacgcccgca aggcgttgga cgcgtatgag cccgccctcc ccctctggcg
     2221 cgggctgggg aaccgggaac gggagatcga caccctcaac cgcctcgggc tctcccactc
     2281 ggccctcggc cggcagaacg aggctctggc cctcttccgg caggccctgg atatcgcccg
     2341 cgatgccgtc gatccctatc gcgaggcgga ggccctggcc aatgtcggct tcatccagca
     2401 ctcccggggc agcctgcggg aggcgctgga gagctacggc cgggccctgg aacggttccg
     2461 ggccgcgggc gatccgtggc aggaggcgac ggtcctcaag aacctgggcg gcgcctggga
     2521 ccggctgggc gagccccagc agagcctgga ccactaccgg caggccctgg acctggcgcg
     2581 cgccctcggc gaccgggccg gcgaggccgg catcctggcc aacctggccg tgacctaccg
     2641 ggagaccggg gagatggggg aggccctggc ccgctatgac gaggccctga ccctcttccg
     2701 ggagctgggg gaccgccgct ggcaggggag ggtcctgaac aacatcggct tcgcctatct
     2761 cctgctggga gaggcgggcc gggccgtccc ctactacgag caggccctgg agctgcggcg
     2821 ggcgacgggg gaccggctgg gggaggccat caccctcaac aacctggggc gcgcccgcgg
     2881 agtgctgggg gagacgcggc aggccctcga cctccaccgc caggcgctgg acctcgcccg
     2941 cgacctcggg gaccggcagg aggaggcgaa cgcgctgggt ctcctggggc aggcccgcgc
     3001 tgcggccggc gacctcgccg gcgcgctcca agacctggga cgcgccgtcg agctgcagcg
     3061 gaccgtcgag gaccgccggc cgctcgcccg cttcctccag cggacgggcg aggtccaggc
     3121 ccgccagggg gacctgccgg cggcgctcgc cagcttcgag caggccctgg ccctgcgccg
     3181 cgccatggcc gaccgctccg gcgcggccga gaccctggac tcgctggccc gcgccgagcg
     3241 cgccgccggc cgcctcgaca ccgcccgggc gcgcgccgaa gaggcgctcg gcgtcatcga
     3301 gtccctgcgc ggagcggtcg agaaccccga cctgcgcgcc tcgttcctcg cctcgcagcg
     3361 ccgggcctac gagctctacg tcgaccttct catggagctg gacggccgcg agccggggcg
     3421 gggctgggcc cgggcggccc tggaggccag cgagcggtcc cgcgcccgca gcctcctcga
     3481 gctgctccac gaggcgggcg ccgacgtgcg cgagggtatc gatcccggcc tccgcgagcg
     3541 ggagcgctca ctgcgccaac ggctgaacgc caaggccctg cggcggctcg agctgctgag
     3601 c
//
LOCUS       DASPJI010000766         9509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_616938, whole genome shotgun sequence.
ACCESSION   DASPJI010000766 DASPJI010000000
VERSION     DASPJI010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9509
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616938"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2120)
                     /locus_tag="VEL74_21440"
     CDS             complement(<1..2120)
                     /locus_tag="VEL74_21440"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG65159.1"
                     /translation="MNPIEDIYRLSPLQEGMLFHSLSDPDAGQYVEQALCTLVGKLDP
                     AALRRGWEAAVARHPVLRASFHWEEVERPVQVVYRQVEVPWREEDWRGLPEEAQAARF
                     EALIDSDRRLGFDLGRPPLLRITLVRLGERTWRFLWSHHHLLLDGWSLARVLREVFAG
                     YAGIAGLPPSRPYVDYIAWLETQDLAEAEAFWRQMLDGFTTPTPLPLSSTSPVRGTPS
                     YEERTLDLPLATQATMEEAARRAGVTLGTMTQAAWALLLARASGEEDVVFGTIVSGRP
                     PHLRGSDAMVGLFINTLPVRVRIEESKELAAWLRGLQDRFLELRGFEHSPLSEVQKWS
                     GVRPGEPLFQALVAFENYPRDVSLQQGTDELKVRDLEVFERTNYPLNLSVVPLDGLQL
                     RLSWDRRRYDLETAERLLHGVRTLLEAMASSLDRPVEDLSLLDDAGRRRVLADAAGPA
                     TPLPDLSMDVLLERAVAERGDAPAIVGPDGTTMSYRELWEKAGGFARRLRELGVRPEV
                     RVGLCMERSPDLIAAMIGVWRAGGAWVPLDPEYPAERLRFLIEDSATPVLVTRQALLE
                     DLPVPDGCRVLSVDDLPSAREEDGEGRERGPAGEGAAYVIYTSGSTGQPKGIVTRHRG
                     LAGFVLEARRLLGLGPESRVPQTTSPSFDASVLEVFPTLLAGGTLFQLSRDTLLSGPG
                     LVEELRRLRITEMAVVPSFLATIP"
     gene            complement(2121..6971)
                     /locus_tag="VEL74_21445"
     CDS             complement(2121..6971)
                     /locus_tag="VEL74_21445"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65160.1"
                     /translation="MSVLLEERLASLPPEKRRLLELRLRKEGIGAARPAGPPAIPRRD
                     PAESGPFPLTFGQQRLWFIEQLNPGTAVYNIPFAGRLRGELDPGLLERSLREVVRRHE
                     VLRTRFRLRDGQPVQVIDAEPFVRLPVIDLTALPADPREAEAARLIVAEPQASFDLER
                     GPVLRVSLVKVSGAQRDEGALRPHVGTLRSQLPGLMGGDTGEPGGPATEHVLLVTMPH
                     LVTDAWSMGIFFRELPAIYEAFRSSRKPDIPELPIQVADYALWQRGWLRGEVLEAQLR
                     FWREYLDGAPPVLTLPTDRPRPPVQTFHGNRLPMALPPEVSRGLRPFNDRRGVTSFMT
                     LLAILNVLLQRWSGLDDVLIGSPVVTRSQSETHPLIGFFINMAVVRTRMQGDLTFEEL
                     LLRVRDSTLASLAHQDLPFERLTDVMEVPRDSPYPPLVQVSYVLQNVHIPQPAFEGIR
                     LVDSWQTDTGMARFDLSVGVFEEEGEETVPQGGFDYNTDLFDETTILRMRGHFLALAA
                     GALAEPERRISELPMHSTAERHQLLVEWNDTAAPSPGRLFHEMVADLARLRPEAPAVV
                     GPDGAIFYAELERRVEVLAARLRRVLRAGKRGSEVGVAVCLERSPERIIAFLAVLTAG
                     GFYLPLDPDWPAERLRYQLRDAGAAVVVTTRELAERLGLGEVELVLLDEDEEGVSGTE
                     DGSEGSPGAGEDSLAYVFYTSGSTGRPKGVMIPHRGLVNLAVAVRRAFGEAPGERVLQ
                     VTAPGFDASVLEMAMALPAGRALHVASPGTLLAGPELAGFAAERAATLGALTPTVLAS
                     VPEESFASLRGVVMGGEACPPELASRWAARVQLFNNYGPPETTVCCTADRVEPGLGGL
                     PRPSIGRTLANLRIDVLDACFQPVPLGAHGEICVGGPSLARGYLGRPELTAASFVPDP
                     FSGEPGSRLYRTGDRARHLPDGRLDFLGRLDGQVKVRGIRIEPGEIEAILQEHPAVRE
                     AAVVLREGEGRLMACVALYEEVSQVELRSWLRERLPEPMMPAAFVLLEALPLTSNHKL
                     DRRALSRLQPEAPDTGKPPNPQDTADLPATDKERLLVAIWASALKRDRVGLHDDFFAL
                     GGDSILAIQVVSRAAEAGLFVEPRQLFAHPTVAGLAAVAAGAPRTDAEQGPVTGPVPL
                     TPVQRWILEDVGLPVPWHWNLSLLLEADGAAIHPAALARAVARLVEHHDQLRARFVRT
                     GAGWRQEVAPLPETPEAGAAPFTLFDLSALPAGVREAAFAAAVGAVQASFDLQRGPLL
                     RAALFTRGADPVRLLLVVHHLVVDGVSWPVLLADLRAAAAGLPLPPKTTSFRRWAERL
                     REYARTAELRQELDVWREAGDRAAPLPADLPAGRSVGPPLEGAAGRVEVRLDAADTWR
                     LVSEVPEAWPVRTEEVLLTAVAEALAEWRGERTAVLAIEGHGRERLFDDVDLSRTVGW
                     LTSTWPLRLELPDGGGPGASLKEVKERVRRVPGRGIGYGLLRHLADDEAAAALRRQPR
                     PDVSFNYLGRTDDPVASSGPFRLAPEPGGGASDPRNPRPHPLAIDAVVLGGELRLVWI
                     YDRGRWLPETIERVAAAALDHLRGLIAHCLGGEASGHTPVDFPHAGLGAEQLDRLMGK
                     IGRQRR"
     gene            complement(6968..>9509)
                     /locus_tag="VEL74_21450"
     CDS             complement(6968..>9509)
                     /locus_tag="VEL74_21450"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG65161.1"
                     /translation="LEQTALAQPALFAVEWSLARLWRDWGVEPEALLGHSVGELVAAC
                     LAGVFSLEDGLALAAARGRLIQETPPGAMLSVPLAEEELAPWLEGGLSLAAVNGPALC
                     AVSGPAEDVERLAARLREAGHESRRLHTSHAFHSALVEPAVAPFRDLVAGIERRPPAI
                     PFLSNVTGDWIRPEDATDPDYWALHLRQPVRFATAAAALLGEPRRLLLEVGPGTTLTT
                     LLRRSGEARERAIPSLPHPHDSETDVEALLGAAGRLWLAGVPLAKALDTAGTRRKVAL
                     PAYPFESERYWIERIEMGGGRQESAAEPLRKRPDVADWFYLPTWKRTLPPGALPAGAA
                     ERESWWIVDPGQGLGERLAERLRDRGRSVTVYAGGEGGEAAPHRVVFLGDAPGLVSLA
                     REIGRRFHSPVHLAVVTRGLYEVTGGEALPGSQSALGAVAGLAKVIPQEYPNLRCRLI
                     DAGPDLPDDILAAELEAEEGPPVVALRGGHRWEQVFEPLRLERREGGPGFDPDAHYVI
                     TGGHGRFGHTVAAYLRDRGARVTLLDLQDGVDVADRAAVARALRDAVARHGRIHGVLH
                     AAGAPADDYRPLSELTAEDLARHLRPKVDGAVAVREALRELGEEPAFCVATSSLAAVL
                     GGVGLAAFAAADAGLDALAGAWGAPWASVNWEVWQAIGGGEAAFLGQQQNELMLTPEE
                     VVETLERVLALRGQPRLAVASGDLEARLAQWTDVLHAGGLTAHARPESGIPYRAPTDP
                     LEARIAEIWQELLGIDRVGVDDDFFLLGGNSLAGLQILSRMRADFDVELPLKAFFEAR
                     TVVGMAGEVRREREKADYEKRRMEEILAEIEGLSLDDVQAQLAVEEGA"
BASE COUNT         1399 a         3525 c         3418 g         1167 t
ORIGIN      
        1 tcggggatcg tcgccaggaa cgacggcacc acggccattt cggtgatccg cagccggcgc
       61 agctcctcca ccagccccgg cccggacagc agggtgtcgc gggagagttg gaacagcgtg
      121 ccgccggcga gcagggtcgg aaagacctcc agcaccgagg catcgaacga tggcgaggtc
      181 gtctgcggca cccggctctc cggccccagg cccaggaggc ggcgcgcctc gagcacgaag
      241 ccggccaagc cgcggtgccg ggtcacgatg cccttgggct ggccggtgga gcccgaggtg
      301 tagatgacat aggcggcgcc ctcccccgcc ggccccctct cccgcccctc cccatcctcc
      361 tctctggcag agggaaggtc atccacgctc aacaccctgc acccatccgg caccggcagg
      421 tcctccagca acgcctgccg ggtcaccagc accggcgtcg cggagtcctc gatcaggaaa
      481 cgtaggcgct cggccggata ctcgggatcg agcggcaccc aggcgccgcc ggccctccag
      541 acgccgatca tcgcggcgat caggtcgggc gagcgctcca tacacaggcc gacccggacc
      601 tcgggccgca cgccgagctc gcgcaggcgg cgggcaaagc cgccggcctt ctcccagagc
      661 tcgcggtagg acatggtggt gccgtccggc cctacgatcg cgggtgcatc cccgcgctcc
      721 gcgacggccc tttcgagaag aacgtccatc gagaggtctg gcagtggcgt ggccgggccg
      781 gccgcgtccg ccaggacacg gcgccggccg gcgtcatcga ggagcgagag atcctccact
      841 gggcggtcga ggctggacgc catcgcctcg agcagcgtgc ggacgccgtg caggaggcgc
      901 tccgccgtct ccaggtcata gcgccggcgg tcccaactga ggcggagctg cagcccgtcg
      961 agagggacga cggagaggtt gagcgggtag ttggtgcgct cgaagacctc caggtcgcgc
     1021 accttcaact cgtccgtgcc ctgctgcagg gagacgtccc gcggatagtt ctcgaaggcg
     1081 acgagggcct ggaagagcgg ctcgccgggg cgcacgccgc tccacttctg gacctcggag
     1141 agcgggctgt gctcgaaacc gcgcagctcc agaaaccggt cctgtagccc ccgcagccag
     1201 gccgccagct ctttgctctc ctcgatccgc acccgcacag gcagggtgtt gatgaacagt
     1261 ccgaccatgg cgtcagagcc acgcaggtgg ggtggccggc ccgagacgat ggtgccgaag
     1321 accacatcct cctcaccgct cgcgcgcgcc agaaggagcg cccaggcggc ctgggtcatg
     1381 gtgccgagcg tcaccccggc gcgccgtgcc gcctcctcca ttgtcgcctg cgtcgcgagc
     1441 ggcaggtcca gggtccgctc ctcgtaggag ggcgtgcctc gcacgggaga ggtggaggac
     1501 aggggcagcg gcgtgggggt agtgaagccg tcgagcatct gccgccagaa cgcctcggcc
     1561 tcggcgaggt cctgcgtctc cagccaggcg atgtagtcga cgtagggccg cgacggcggc
     1621 aggccggcga tgccggcgta cccggcgaag acctcgcgca gcacccgcgc caacgaccag
     1681 ccgtcgagca gcaggtggtg gtgactccac aggaagcgcc aggtgcgctc gccgaggcga
     1741 accagggtga tgcgcagcag cggcggacgg ccgaggtcga acccgagacg gcggtcgctg
     1801 tcgatcagcg cttcgaaacg cgccgcctgc gcctcttccg gcagcccgcg ccagtcctcc
     1861 tcgcgccagg ggacctccac ctgccggtag accacctgca ccgggcgttc cacctcttcc
     1921 cagtggaacg acgcgcgcag cacgggatgg cgcgccacgg ccgcctccca cccccggcgc
     1981 agcgccgcgg ggtccagctt tcccaccagc gtgcagaggg cctgctcgac gtactggccc
     2041 gcgtccggat cgctcaggct gtggaacagc atcccctcct gcagagggga gagacggtag
     2101 atgtcttcga tgggattcat ctatctccgt tgcctcccga tcttccccat cagccggtcg
     2161 agctgctccg ctccgagccc ggcatgcggg aagtccacgg gcgtatgacc ggacgcctcg
     2221 ccgcccagac agtgggcgat cagtccgcgc aaatggtcga gcgccgccgc cgccacccgc
     2281 tcgatggtct cggggagcca gcggccgcgg tcgtagatcc agaccaggcg cagctcgcca
     2341 cccagcacca cggcgtcgat cgccagtgga tgcgggcgcg ggttgcgcgg atcgctcgct
     2401 ccgccccctg gctcgggcgc caaccggaac ggaccggagc tggccacagg gtcgtccgtc
     2461 cggcccaggt agttgaacga gacgtcgggc cgcggctggc ggcgcagggc cgcggcggct
     2521 tcgtcgtcgg cgaggtgacg gaggaggccg tagccgatgc cgcgcccagg tacccgccgc
     2581 acccgctcct tgacctcctt gagcgacgcg ccggggccgc cgccgtccgg cagctccagc
     2641 cgcagcggcc aggtggaggt gagccagccc accgtgcgcg acaggtcgac gtcgtcgaac
     2701 agccgctcgc ggccgtgccc ctcgatggcg aggacggccg tgcgctcgcc gcgccactcc
     2761 gccagggcct cggccaccgc ggtgagcagc acctcctcgg tacgtaccgg ccaggcctcc
     2821 ggcacctcgg agacgagccg ccaggtgtcc gcggcgtcga gccgcacctc cacccggccg
     2881 gccgcgcctt ccagcggcgg gcccacgctg cggccagcgg gcaggtcggc cggcagcggc
     2941 gcggcgcggt cgccggcctc gcgccagacg tccagctcct ggcgcagctc cgcggtgcgg
     3001 gcgtactcgc ggagccgctc ggcccagcgc cggaacgagg tggtcttcgg cggcaagggg
     3061 agccccgccg cggcggcgcg caggtcggcg agcaggaccg gccaggagac gccgtccacc
     3121 accaggtgat ggaccacgag gaggagccgg accggatcgg cgccccgagt gaacagcgcg
     3181 gcgcgtagga gaggaccgcg ctggaggtcg aagctcgcct ggaccgcgcc gaccgccgcc
     3241 gcgaaggcgg cctcccgcac gcctgcgggc agggcggaga ggtcgaacag cgtgaacggc
     3301 gccgcgccgg cctccggcgt ttccggcagc ggcgcgacct cctggcgcca gccggcgccg
     3361 gtgcggacga agcgggcgcg gagctggtcg tggtgctcga ccaggcgcgc caccgcccgg
     3421 gcgagcgccg ccggatggat cgcggccccg tccgcctcca gcagcaggga gaggttccaa
     3481 tgccagggta ccggcaggcc cacgtcctcc aagatccagc gctggaccgg cgtcagcggg
     3541 acggggccgg tgaccggccc ctgctcggca tcggtgcgtg gcgcgcccgc ggccaccgcc
     3601 gccaggccgg ccaccgtcgg gtgcgcgaag agctgccgcg gctcgacgaa cagcccggcc
     3661 tcggcggcgc gcgagaccac ctggatcgcg aggatcgagt cgccccccag ggcgaagaag
     3721 tcgtcgtgga ggccgacgcg gtcgcgcttc agggcgctgg cccagatggc cacgagcagc
     3781 cgctccttgt ccgtcgccgg caggtccgcc gtgtcctgcg ggttcggcgg cttcccggtg
     3841 tccggcgcct cgggctggag ccgggagagg gcgcgccggt ccagcttgtg gttcgaagtg
     3901 agcggcagcg cctcgagaag gacgaacgcg gccggcatca tgggctcggg gaggcgctcc
     3961 cgcagccagc tcctcagctc gacttggctc acctcctcgt acagcgccac gcaggccatc
     4021 agccggccct ccccctcacg caggacgacc gccgcctcgc gcaccgcggg gtgctcctgt
     4081 aggatagcct cgatctctcc cggctcgatg cggatcccgc gcaccttgac ctggccgtcc
     4141 aagcggccga ggaagtccag ccgcccgtcc gggaggtggc gggcgcggtc gccggtgcga
     4201 tagagccgtg agccgggctc gccggagaag gggtcgggca cgaagctggc ggccgtcagc
     4261 tccggtcggc cgaggtagcc gcgcgccagg ctggggccgc cgacgcagat ctcgccgtgc
     4321 gcgccgagcg ggaccggctg gaagcaggcg tccagcacgt cgatgcggag gttggcgaga
     4381 gtccggccga tcgatggacg cggcaggcct cccagcccgg gctcgacccg atccgccgta
     4441 cagcagaccg tcgtctcggg ggggccgtag ttgttgaaca gctggactct cgcggcccag
     4501 cgcgacgcca gctcgggcgg gcaggcctca ccgcccatga cgacaccgcg caggctggcg
     4561 aacgactcct ccggcacgct ggccaggacg gtgggggtca aggcgccgag ggtggcggcg
     4621 cgctccgccg cgaagccggc gagctccggg ccggcgagca gcgtgcccgg gctagcgacg
     4681 tgcagcgccc ggccggccgg aagagccatc gccatctcga ggaccgaggc gtcgaagccg
     4741 ggcgcggtca cctgcagaac ccgctcgccc ggagcctccc cgaaagcacg ccgcacggcc
     4801 acggccaggt tgaccagccc gcggtgaggg atcatcaccc ccttgggccg gccggtggag
     4861 ccggaggtgt agaagacgta ggcgagggaa tcctccccgg cccctgggct cccctcactc
     4921 ccatcctccg tcccactgac tccttcctcg tcctcatcca gcagcaccag ctccacttcc
     4981 ccgagaccga gccgctccgc cagctcccgc gtcgtgacga cgaccgccgc tcccgcgtca
     5041 cggagctggt accgcaggcg ctcggcaggc cagtccggat cgagcggcag gtagaaaccg
     5101 cccgcggtca ggaccgccag gaaggcgatg atccgctcgg gcgagcgctc caggcagacc
     5161 gccaccccca cctcggatcc acgctttcca gcacgcagaa cacggcgcag gcgcgcggcg
     5221 aggacctcca cccgcctctc cagctcggcg tagaagatcg cgccgtcggg tcccaccacg
     5281 gccggggcct ccgggcgcag acgggccagg tcggccacca tttcgtgaaa gaggcgcccc
     5341 ggcgacggag cagcggtgtc gttccactcc accaggagct ggtggcgctc cgccgtggaa
     5401 tgcatcggca gctcggagat ccggcgctcc ggctcggcca gggcccccgc cgccaacgcc
     5461 aggaagtggc cacgcatgcg caggatcgtg gtctcgtcga acaggtcggt gttgtagtcg
     5521 aagccgccct ggggaacggt ctcctccccc tcctcttcga acacgccgac gctgaggtcg
     5581 aaacgggcca tgccggtgtc ggtctgccag gagtcgacga gccggatgcc ctcgaaagcc
     5641 ggctgcggga tgtggacgtt ctgcagcacg tagctgacct gcaccagcgg cgggtacggg
     5701 gagtcgcgcg gcacctccat cacgtcggtg agccgctcga aagggaggtc ctggtgcgcc
     5761 aggctcgcca gcgtgctgtc gcggacgcgc agcagcagct cctcgaacgt cagatcacct
     5821 tgcatccgcg tgcgcaccac ggccatgttg atgaagaagc cgatcagcgg atgggtctcg
     5881 gactgtgatc gggtcaccac cggtgagccg atcaagacat cgtcgaggcc gctccagcgc
     5941 tggagcagca cattgaggat cgcgaggaga gtcatgaagc tggtaacgcc gcggcggtcg
     6001 ttgaacgggc ggagaccgcg gctcacctcc ggcggcaggg ccatgggcag gcgattgccg
     6061 tggaaggtct ggaccggcgg cctcggccgg tcggtgggca gcgtcagcac cggcggcgcg
     6121 ccgtcgaggt actcgcgcca gaagcggagc tgcgcctcca gcacctcgcc ccgcagccag
     6181 ccacgctgcc agagggcata gtcggccacc tggatcggca gctcggggat atccggcttc
     6241 cggctacttc ggaaagcttc gtagatcgcc ggcagctcgc ggaagaagat gcccatcgac
     6301 caggcgtccg tcaccaggtg gggcatggtg accaggagga cgtgctccgt ggccggcccg
     6361 ccgggctcgc cggtgtcgcc gcccatcaga ccggggagct gggagcggag cgtgccgacg
     6421 tggggccgga gagccccctc atcccgctgg gcgccgctca ccttgaccag gctgacgcgc
     6481 agcaccgggc cgcgctccaa gtcgaaggag gcctggggct cggccacgat gagccgcgcc
     6541 gcctcggcct cgcgcggatc ggccggcagc gccgtcaggt cgatgaccgg cagccgcacg
     6601 aacggctcgg cgtcgatcac ctgcacaggc tgaccgtcgc ggagccggaa gcgggtgcgg
     6661 agcacttcgt gccggcggac cacctcgcgc aggctacgct ccagcaaccc cggatcgagc
     6721 tcgccgcgca accggcccgc gaaggggatg ttgtagaccg ccgtgcccgg gttgagctgc
     6781 tcgatgaacc acagccgctg ctggccgaag gtgagcggga acggcccgga ctcggcaggg
     6841 tcgcgccgcg ggatcgccgg cggccccgcg ggccgtgcgg cgccgatccc ctccttgcgc
     6901 aggcgcagct cgagaagccg ccgcttctcg ggcggcagcg acgccaggcg ctcctccaac
     6961 aggacgctca agccccctcc tcgacggcga gctgcgcctg gacgtcgtcc agcgacaggc
     7021 cctcgatctc ggccaggatc tcctccatcc gccgcttctc gtagtcggcc ttctcccgct
     7081 cgcgccggac ctcgccggcc atacccacca cggtgcgggc ctcgaagaag gccttgagcg
     7141 gcagctcaac gtcgaagtcg gcgcgcatcc tggaaaggat ctgcagtccg gccaacgagt
     7201 tgccgccgag caggaagaag tcgtcgtcga cgccgacgcg gtcgatgccc aggagctcct
     7261 gccagatctc ggcgattcgc gcctcgagcg ggtcggtcgg cgcgcggtag gggatgcccg
     7321 actcggggcg cgcgtgggcg gtcagaccgc cggcgtgcag gacgtccgtc cactgcgcga
     7381 ggcgcgcctc caggtcgccg ctcgcgaccg cgagccgcgg ctgaccgcgc agggccagca
     7441 cccgctccag ggtctcgacc acctcctccg gcgtcagcat caactcgttc tgctgctgcc
     7501 cgaggaaggc cgcctcaccc ccgccgatcg cctgccagac ctcccagttg acgctggccc
     7561 agggagcgcc ccaggcgccg gccagggcat cgaggccggc atcggcggcc gcgaaggcgg
     7621 ccaggccgac gccgccgagc accgcggcga gcgacgaggt ggcgacgcag aacgcgggct
     7681 cctcgcccag ctctcgcaac gcctcgcgca cggcgacggc cccgtccacc ttgggccgca
     7741 ggtgcctggc caggtcctcg gcggtcagct cggagagtgg acggtagtcg tccgccggcg
     7801 cgcccgccgc gtgcagcacg ccgtggatcc ggccgtgccg ggccacggcg tcgcgcaggg
     7861 cgcgcgccac ggccgcgcgg tcggccacgt cgactccgtc ctggagatcg aggagtgtga
     7921 cccgggcgcc gcggtcccgc aggtacgccg cgaccgtgtg cccgaagcgc ccgtggccgc
     7981 cggtgataac gtagtgcgcg tccgggtcga agcccggccc gccttcgcgc cgctccagcc
     8041 gcagcggctc gaacacctgt tcccagcggt ggccgccgcg cagtgcgacg accggcgggc
     8101 cctcctcggc ttccagttcg gcggccagga tatcatccgg caggtcgggg ccggcgtcga
     8161 tcagccggca gcgcaggttg gggtactcct gcgggatgac cttggccaga ccggcgacgg
     8221 cccccagggc cgactgactg ccggggagcg cttcgcctcc ggtgacctcg tagaggccgc
     8281 gggtgaccac cgcgaggtgg accggcgagt ggaagcgccg gccgatctcg cgggccaggg
     8341 agacgaggcc tggcgcgtca ccgaggaaaa ccacacggtg cggcgccgcc tcccctccct
     8401 cccctccggc gtacacagtg acggacctgc cccggtcgcg gaggcgctct gccagccgct
     8461 cgcccagacc ctgtcccgga tcgacgatcc accagctctc gcgctccgcc gctccggcag
     8521 ggagagcgcc cggcggcagc gtccgcttcc aggtggggag atagaaccag tccgcgacgt
     8581 ctggccgctt gcggagaggc tccgccgcgg attcctgacg cccgccaccc atctcgatcc
     8641 tctcgatcca ataccgctcg ctctcgaagg ggtaggccgg cagcgccacc ttccgccgcg
     8701 tcccagcggt gtcgagggcc ttcgccaggg gcacccctgc cagccagaga cggccggcgg
     8761 ctccgagcag cgcttccacg tcggtttcgg agtcgtgcgg gtgcggcagc gaggggatcg
     8821 cccgttcccg ggcttctccg ctccggcgca gcagagtggt caaggtggtt ccagggccga
     8881 cctccaggag cagccgccgc ggctcgccga gcagggccgc ggccgccgtc gcgaagcgga
     8941 ccggctggcg caggtggagc gcccagtagt ccggatcggt cgcgtcctcg gggcggatcc
     9001 agtcgccggt gacgttggac aggaagggga tggctggcgg ccggcgctcg atccccgcaa
     9061 ccagatcacg gaagggggca accgcgggct cgaccagcgc cgagtggaac gcatgcgagg
     9121 tgtgcagacg gcggctctcg tgccccgctt cgcgcagccg cgccgccaac cgctcgacat
     9181 cctcggccgg gccggagacg gcgcacaggg ccgggccgtt gaccgcggcc agcgacagac
     9241 cgccttcgag ccagggcgcc agctcctcct ccgcgagcgg caccgagagc atggcgccgg
     9301 gcggcgtctc ctggatcaac cggccgcggg ccgccgccaa cgccagcccg tcctccagcg
     9361 agaagacgcc ggcgaggcag gcggcgacga gctcgccgac cgagtggccg agcagcgcct
     9421 cgggctcgac gccccagtcc ctccacaggc gggccagcga ccattcgacg gcgaacaggg
     9481 ccggctgcgc cagcgcggtc tgctccagc
//
LOCUS       DASPJI010000767         4561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_49608, whole genome shotgun sequence.
ACCESSION   DASPJI010000767 DASPJI010000000
VERSION     DASPJI010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49608"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..490
                     /locus_tag="VEL74_21455"
     CDS             89..490
                     /locus_tag="VEL74_21455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG65162.1"
                     /translation="MHPLPHPAHRLLIVDDEEAILFAMTDYFSRSGYLVDCARSRGEA
                     EDLLASEAYSLVIADLRLDPADPRGGLALLLRARQSRGGTRGILLTAYGSTEVESELL
                     GMGGALLLSKPRPLAQVGEAVSRLLDGLPPT"
     gene            700..1776
                     /locus_tag="VEL74_21460"
     CDS             700..1776
                     /locus_tag="VEL74_21460"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYG65163.1"
                     /translation="MLSYRDKSLWSLSVLLSAEDSAAARRSQELLARAKRTRFEVMPT
                     ERLDQAVEKLRCRAYDLMVLDLDLTREPGHEALLRAQTLAHRLPIVVMSAQENEDLAL
                     RAVEAGIQDFIIREPWQPEALERIMRHAVVRHRLVAAMRRAHITSTARPLFDAQTGLA
                     TEAAFTRRLQETLELSHRFGQRPALLVLELDRFRKVAGRLGPLAASRILEEVGRRLTW
                     CVRRSDLTGRLGEERFAVMLPNTPGSWAVRPVAERLRLAVGAPFDLDATQSRLSGSVG
                     TACFPHDGETAADLLASADAAILEAKNLGGNRCCFFRSLDVPPWPGAEPGDSPAGFPS
                     LLGAASQAVASFKAGGSLAAGERG"
     gene            1773..>4561
                     /locus_tag="VEL74_21465"
     CDS             1773..>4561
                     /locus_tag="VEL74_21465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYG65164.1"
                     /translation="MKNRRSQAAERAALERENRELRSFRRVSEIALTHPPLASLYEQF
                     LEEVSVSSGFPFVVLEAYEPVRELLRCEAVWGVPIPELRGVETPVEGSLAGSAVSSAG
                     SRVERLSLDDAAARPLALAGVELRTCVYVPMRVEGGIFGVLSLGSGEALAPDDRFLRW
                     LEDLGGQIAMLLERKRYEEERRCLLRDLNERVKELRTLHETSRLLQSHQGSTPELLQR
                     LAWLLPFAWQYPETTEVRIRYGEIEGATTGFLPSPWCQQAAFHTADGTAGRIEVVYLE
                     ERPPSAEGPFLAEERYLLESLAEMLRNHLDRLEAEHKVQASESRLRLVVEQMPAVLWT
                     TDAELRFTSCRGVGLSGLGLPPGEIEGRGVIDFYSDGDNNAVPAHLRALGGESVSYSQ
                     ELRERLFECHVEPLRDAEGRIDGCIGAAMDVTVRKQAEEELRVLSETLEALVEASPLA
                     ILALDEESRVTLWSPAAERTFGWRRDEVLGLRLPTVPGDRWDEHLSVRGSVLAGERFT
                     GIETRRCDRAGAVIDVRISVAPLRDAGGRVRGMAEFLEDITDRRRAERAIRRLASLPE
                     QSPDPLVELDFAGNALYVNQAARLRFPDLQALGTWHPVLSQVSPILARFRQGDRKSFS
                     FEVGYEGSVYHQMIYYVPDGHVVRVFLHDVTEQRRVQALLELQASRDGLTGLANRESF
                     LRRVEERLRKEGAPPCAVLAIDLDRFKVINDSLGHAAGDELLTAVGQRIAACLGTGDS
                     AARLGGDEFAVLLRETLGTSEAFQVAERLCEAVRLPLELCGQEVFPSAAVGIAPGEGY
                     RDSELLLRDAHVAMYQAKALGGGRCEVFDPVRGRRSFDRLCLENELRRAVERRELELV
                     YQPIVSLLDGSITAFEALMRWPRQERGAVSPAEFIPIAEESGLILPLSYWALGEVCQR
                     LREWSGPAGDGDPVS"
BASE COUNT          615 a         1592 c         1660 g          694 t
ORIGIN      
        1 gcgccgtctc ggaatccccc gcagcacgct gtaccagaag atcaaggact acgccgtgga
       61 cctcgccagg ttccagggtt gatctcccat gcatcccttg ccgcaccccg cccaccggct
      121 gctgatcgtc gacgacgagg aggcgatcct cttcgcgatg accgattact tcagccgctc
      181 cggctacctg gtggattgcg cgaggagccg cggcgaggcc gaggatctcc tggcctccga
      241 ggcgtactcg ctggtcatcg ccgacctgcg gctcgacccg gcggaccccc ggggcggcct
      301 cgccctgctg ctccgggcga ggcagagccg tggcggcacg cgcgggatcc tcctcacggc
      361 ctacggctcc acggaggtgg agtccgagct gctcgggatg ggcggcgccc tgctgctctc
      421 caagccccgg cctctcgcgc aggtcggcga ggccgtcagc cgcctcctcg acgggctccc
      481 gccgacgtga tggtgatgcg gtccggtccc gcgtccagcg cttggacacg cctgtccaga
      541 agctggagcg gacggacgga ccgccgccgg ccctcgccgt taccggcagg gccaacccct
      601 ttgcttccaa ggtttccccg cgcctccgga gaggccgccg agctggaaca cggattgcac
      661 gatcaggctc tacagaaacc actggatgtc ggaggttgca tgttgtcgta cagagacaag
      721 agcctgtgga gtctctccgt cctgctgtcg gccgaggact ccgccgcggc gcggcggtcg
      781 caagagctgc tggcgagagc caagcgcaca cgattcgaag tgatgccgac ggagcgcctc
      841 gaccaggcgg tcgagaagct gaggtgccgc gcctacgacc tgatggtcct ggacctcgac
      901 ctgacgcggg agcccggaca cgaggcgctc ctgcgggcgc agacgctcgc tcatcgcctg
      961 cccatcgtgg tgatgagcgc gcaagagaac gaggatctgg cgctgcgcgc ggtggaagcc
     1021 gggatccagg atttcatcat ccgggagccc tggcagccgg aggccctcga aaggatcatg
     1081 cggcacgcgg tggtccgcca ccggctcgtg gccgcgatgc ggcgggccca tatcacctcc
     1141 accgcccggc cgctgttcga cgcgcagacc gggctggcca ccgaggccgc cttcacgcgc
     1201 cgcctgcagg agacgctgga gctgtctcat cgtttcggcc agcggcccgc cctgctggtc
     1261 ctcgagctgg accgcttccg caaggtcgcg ggccgtctcg gtccgctggc cgcctcgcgg
     1321 atcctggagg aggtcggccg ccgcctcacc tggtgcgtgc gccgcagcga cctcaccgga
     1381 cggctgggcg aggagaggtt cgcggtcatg ctccccaaca cgccgggcag ctgggcggtg
     1441 cgccccgtgg cggagcgcct ccgcttggcg gtgggggcgc cgttcgacct cgacgcgacg
     1501 cagtcccgtc tctcgggcag cgtgggcacc gcctgtttcc cccatgacgg agagaccgcg
     1561 gcggatctcc tggcgagcgc cgatgccgcg atcctggaag ccaagaacct gggaggcaac
     1621 cgctgctgtt tcttccgcag cctggacgtc cctccatggc ctggggcgga gccgggggac
     1681 tctccggcgg gtttcccgtc gctgctgggg gctgcctccc aggcggtcgc gtcgttcaag
     1741 gccggcggct cgctcgccgc tggggagcgg ggatgaagaa ccgccggtcc caggcggccg
     1801 aacgggccgc cctggagagg gagaaccgtg agctgcggag cttccggcgc gtctccgaga
     1861 tcgccctcac gcatccgccg ctggcctcgc tctacgagca gttcctggag gaggtcagcg
     1921 tctccagcgg ttttccgttc gtcgtgctcg aggcctatga gccggttcgc gaactgctgc
     1981 gctgcgaagc ggtctggggc gtgccgatcc cggagctgcg gggggtggag acgccggtgg
     2041 aggggagcct ggcgggcagc gccgtgagct ctgcggggtc acgagtggag cgcctgtctc
     2101 tggacgatgc cgcggcgcgc cccctggcgc tggccggagt cgagctgcgg acctgcgtct
     2161 atgtgcccat gcgggtggag ggggggatct tcggcgtgct cagcctgggc agcggcgagg
     2221 cgctggcccc ggacgaccgc ttcctgcgct ggctcgagga cctgggcggg cagatcgcca
     2281 tgctcctgga gcgcaagcgc tacgaggaag agcggcggtg cctgctgcgc gacctcaacg
     2341 agcgggtcaa ggagctcagg actctgcacg agacctcacg gctcctccag agccatcagg
     2401 ggtccacgcc ggagctgcta cagcggctgg cctggctcct gcccttcgcc tggcagtacc
     2461 ccgagaccac ggaggtgcgt atccgctacg gcgagatcga aggcgcgacc accggcttcc
     2521 tgccgtcccc ctggtgccag caggcggcct tccataccgc cgacggcacc gccggccgca
     2581 tcgaggtggt ctacctggag gaacggccgc ccagcgccga aggccctttc ctggccgagg
     2641 agagatacct cctggagtcg ctggcggaga tgctccgcaa tcacctggac cgcctggagg
     2701 ccgagcacaa ggtgcaggcg agcgagtccc ggctccgcct ggtggtggag cagatgcccg
     2761 ccgtgctgtg gacgacggac gccgagctcc gcttcacctc ctgccgcggg gtgggcctgt
     2821 ccggcctggg cctccctccc ggcgagatcg agggccgtgg cgtcatcgac ttctactccg
     2881 atggggacaa caacgcggtg ccggctcacc tgcgggcgct cggcggcgag tccgtgagct
     2941 actcgcagga gctgcgggag cggctcttcg agtgtcacgt ggagcccctg cgggacgcgg
     3001 aggggcggat cgacggatgc atcggcgcgg ccatggacgt gaccgtccgc aagcaggccg
     3061 aggaggagct ccgggtgctc tcagagactc tcgaagcgct ggtcgaggcc tcgccgctgg
     3121 ccatcctcgc cctggacgag gagagccggg tcacgctgtg gagcccggcg gccgagcgca
     3181 ccttcggctg gcgccgggac gaggtcctgg ggctgcggct ccccacggtg cccggcgacc
     3241 gctgggacga gcacctgagc gtgcgcggca gcgtcctcgc gggcgagcgc ttcaccggca
     3301 tcgagacccg ccgctgtgac cgggccggag ccgtgatcga cgtccggatc tcggtagcgc
     3361 cgctacgcga tgccggcggc cgggtgcgcg gcatggccga gttcctggag gacatcaccg
     3421 accgccggcg ggccgagcgc gcgatccgcc gcctcgcctc gctccccgag cagagcccgg
     3481 atccgctggt ggagctcgac ttcgccggca acgccctcta cgtcaaccag gccgcgcggc
     3541 tccgcttccc ggacctccag gcgctcggca cgtggcaccc ggtgctgtcc caggtctccc
     3601 cgatcctcgc gcgcttccgt cagggggacc gcaagtcctt ctccttcgag gtgggctacg
     3661 aagggtcggt ctaccaccag atgatctatt acgtgccgga cgggcacgtg gtgcgggtgt
     3721 tcctgcacga cgtcaccgag cagcgccggg tccaggcgct gctcgagctc caggcctccc
     3781 gggacggtct gaccgggctg gccaaccggg agtctttcct gcgccgggtg gaggagcggc
     3841 tacgcaagga aggcgcgccg ccctgcgccg tcctggcgat cgatctggac cgcttcaagg
     3901 tgatcaatga cagcctgggg cacgccgcgg gggacgagct gctgaccgcc gtggggcaac
     3961 gcatcgccgc ctgtctgggg accggcgact cggcggcacg cctggggggg gacgagttcg
     4021 ccgtgctgct gcgggagacc ctcggcacct ccgaagcctt ccaggtcgcc gagcggctct
     4081 gcgaggcggt gaggctccct ctggagctgt gcggccagga ggtcttcccc agcgcggcgg
     4141 tcggcatcgc gccgggcgag ggctaccggg attccgagct cctgctgcgg gacgcccacg
     4201 tggccatgta tcaggcgaag gccctgggtg gtgggcgttg cgaggtcttc gacccggtgc
     4261 gggggcgccg ctccttcgac cggctctgcc tcgagaacga gctgcgccgg gccgtcgaga
     4321 ggcgcgagct cgagctcgtc tatcagccga tcgtcagcct gctcgacggc tccatcacgg
     4381 ccttcgaggc gctgatgcgc tggccgcggc aggagcgggg ggcggtttcc ccggcggagt
     4441 tcattcccat cgccgaggag tccgggctga tcctcccgct ctcctactgg gccctgggag
     4501 aggtctgcca gcgcctgcgg gagtggagtg gcccggccgg agacggcgac ccggtctcca
     4561 t
//
LOCUS       DASPJI010000768         8782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_220575, whole genome shotgun sequence.
ACCESSION   DASPJI010000768 DASPJI010000000
VERSION     DASPJI010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8782)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8782
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220575"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VEL74_21470"
     CDS             complement(<1..858)
                     /locus_tag="VEL74_21470"
                     /inference="COORDINATES: protein motif:HMM:NF024566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HYG65165.1"
                     /translation="MVSPELARHLTETSQRLGRQVGVLLSRAGEVRNVIVGDAHSLTL
                     PDVGRLRGGVGRFRGLRLVHTHLKGEPLTNDDLNDLALLRLDMVAALEVLPTGLPGRV
                     DIAHLAPFDAAATDAAAANGDGLGVVSEPFERIQAPDVHQLDLDFEATMRALEEEFAR
                     VTSEAGEVEQERALVVGIVPDDEHFAETLELVRSADVTIAGTVRQKRSQVHPRTVVGR
                     GKLQDIVLEGMRRRASVAIFDIDLKPAQARAFEDATGLKAIDRTQLILDVFAQRARSR
                     DGKLQVELAQ"
     gene            complement(964..1638)
                     /locus_tag="VEL74_21475"
     CDS             complement(964..1638)
                     /locus_tag="VEL74_21475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HYG65166.1"
                     /translation="MADFTVPAVIPVFPLTGTLLLPGTLLPLNIFEPRYRNMVADALE
                     GERAIGMIQPFVPRQDNWPALELPPENPELYPVGCLGRMERCEPQPDGRYEIVLRGIC
                     RFRIREELPLHRGYRRVRADTAEFAADLNEPASALDPSRLLAAVRTFGASNGLDFDLD
                     LLSAIPGVSLLNGLSVALPFRPAEKQALLEAGGPEERERLLLALMGMGLEPMAPEEYY
                     APPTVH"
     gene            complement(1689..2624)
                     /locus_tag="VEL74_21480"
     CDS             complement(1689..2624)
                     /locus_tag="VEL74_21480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017609329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HYG65167.1"
                     /translation="MAVRHVISIADLDTDTLSRLVDDAVSIARGEWAGKRPLQDKVVG
                     IYFKKTSTRTRTSFNVGAMKLGADVIAYGPNDLQLNTGETIEDTARVLANYMEILVLR
                     TNEPMEEMRQLAAASDRLSIVNAMSDNEHPTQAICDLAALKEALGRLEGVHMLFMGEG
                     NNTASALALAFGMLPKMKLTLVTPEGYGLPKEDMDRALRYAAKTGSEIVEHHDLDKLP
                     KDVDAVYTTRWLTMGVQKKDEDWLDKFRPYTVTPQVMERVSKQGTIFLHDLPAMRNYE
                     VLDAVLDGPQSIAFRQAYHKMTSAMAVLNWCAEGR"
     gene            complement(2744..4543)
                     /gene="lepA"
                     /locus_tag="VEL74_21485"
     CDS             complement(2744..4543)
                     /gene="lepA"
                     /locus_tag="VEL74_21485"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007334775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HYG65168.1"
                     /translation="MDNIRNFCIIAHIDHGKSTLADRLIQRCGAVELRDFRDQILDSM
                     DIERERGITIKSNTVSLVYTAKDGRDYHLNLIDTPGHVDFSHEVRRSLMSCEGALLIV
                     DASQGVEAQTVANLYLALEYDLEIVPVINKIDLPSAEVDRVREEIDADLGLDPFEAVL
                     CSAKQGIGIDDVLEAVVHKLPAPQGDPADPLRALIFDAQYDPYRGVVLLCRVKEGTVR
                     PKQTIRFIHTGKDYQVEEVGLLRLKRVKTDEIVAGDVGYVIAGVKAVSEITVGDTITE
                     ADRPAAEPLPGYKEAKPVVFSSIYPIATDDYEDLAKALDKLKLNDAALTFQKDSSSAL
                     GFGFRCGFLGLLHLEVVQERLEREYGLSLLLTAPSVQYEITLADDKIVMIDNPALFPD
                     PGTIEKAREPYIRAAIMIPERYLGVVMELCRERRGTNNEVQFLAVGRQQVVSELPLAE
                     ILFDFYDRLKTITQGYGSFDYEMLDLRETDLVKVDILVNGERVDALSQLVHREKARQR
                     ALHYCERLEATIPRQQFKIAIQGAIGGTIIARTTINAFRKDVTAKCYGGDITRKRKLL
                     EKQKEGKKRMKMVGAVEIPQEAFLAVLKSDTDS"
     gene            complement(5314..>8782)
                     /gene="pulA"
                     /locus_tag="VEL74_21490"
     CDS             complement(5314..>8782)
                     /gene="pulA"
                     /locus_tag="VEL74_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA];
                     GO_process: GO:0005983 - starch catabolic process
                     [Evidence IEA];
                     GO_process: GO:0051678 - pullulan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pullulanase-type alpha-1,6-glucosidase"
                     /protein_id="HYG65169.1"
                     /translation="GCSGDWQPDCTETGLTYDAADGVWQGTFDLPAGDWEYKAALDGS
                     WDVNYGANATLNGPNIGLSLSDPAAVKFYYSHDTHWVTSNRNAVIATAAGSFQSEIGC
                     PGDWQPDCLRSWLQDPDGDGLHTFSTRLIPPGNYEVKVAIDESWSENYGAGGARDGAN
                     IAFSVPAACVEVFFTYDATSHVLTVGTSSQGPRGNITRAQAHWVTQDTIAWKVPAGSE
                     VTLHHAPEGGMTLTSDGVQGGTGIQLIHHPAGLPAAVLEKFPHLAGSAAFKIPAEHLD
                     EVAEILKGQIAVSASTGDGTLVDATALQIPGVLDDLYSYDGPLGATLAGTVPTLRVWA
                     PTAQSVKLHVFANSNPATPSTVVPMTLDPATGVWSVTGEASWYGRYYLYEVEVFVRAT
                     GEVETNLVTDPYSVSLSINSRRSQIISLADPALEPAGWDALVKPPLAAPEDITLYELH
                     LRDFSANDATVSPVFRGTYKAFTLPGTNGMRHLASLAQAGLTHVHLLPVFDIATIDED
                     KSTWLSPAGDLASYPPDSEQQQAAVMAVADRDAFNWGYDPWHYTVPEGSYSTDPDGPA
                     RVREFREMVAGLSGIGLRVVMDVVYNHTNAAGQNDKSVLDRIVPGYYHRLNADGNVET
                     STCCQNTATEHDMMEKLMVDSVVTWAKAYKVDGFRFDIMGHHMKRNMLAVRAALDALT
                     LAADGVDGRKIYVYGEGWNFGEVANNARGEQATQINMAGTGIGTFNDRLRDGARGGGP
                     FSGLQEQGFLTGLYYDPNSTDQGSASDQLAALLHRTDWIRSGMAGGLAEYELVDSQGN
                     LVEAREIDYNGQPTGYTADPQEIINYVEAHDNETLFDIIQLKAPVTATMADRVRMQNL
                     GMDLLAFGQGIPFFHAGVELLRSKSLDRNSYNSGDWFNRLDFTYQDNNFGVGLPPAAD
                     NQNNWPVMAPLLANPALQPAPEDIQRAYHHFREVLMIRRSSPLFRLRTAEQIEDRLAF
                     ANTGPNQIPGVIAFTLTDTDGTVDRRHKMIVTVINANDQAQSVQASVFACPNLTLHPV
                     LANSADAVVRLATYNPAICTFQVPARTSVVFWQVRPAAEQIVLLQQDVTNLVNTGVLN
                     NGQGNALLAKLRAAAQQVGRGNTTPAINQVNAFINQVEDFAAEGLLTADQAAALTDAA
                     EGILEALGA"
BASE COUNT         1243 a         2866 c         3171 g         1502 t
ORIGIN      
        1 ctgcgcgagc tccacctgca gcttgccgtc gcggctgcgg gcacgctggg cgaagacgtc
       61 gaggatgagc tgcgtgcggt cgatcgcctt caaccccgtg gcgtcctcga aggcgcgggc
      121 ctgggcgggc ttgaggtcga tgtcgaagat ggcgacgctc gcccgccggc gcatcccttc
      181 gagcacgatg tcctgcagct tgccgcggcc gaccaccgtc cgggggtgga cctgggagcg
      241 cttctggcgc accgtgccgg cgatcgtcac gtccgccgag cgcaccagct ccagcgtctc
      301 ggcgaagtgc tcgtcgtccg ggacgatgcc caccaccagg gcgcgctcct gctccacctc
      361 gccggcctcg ctggtgacgc gggcgaactc ctcctccagg gcccgcatgg tggcctcgaa
      421 gtcgaggtcg agctggtgca cgtccggcgc ctggatgcgc tcgaacggct cgctgaccac
      481 gccgaggccg tcgccgttgg ccgccgccgc atccgtcgcc gccgcgtcga atggcgcgag
      541 gtgggcgatg tcgacccggc cgggcagccc ggtggggagg acctcgagcg cggcgaccat
      601 gtccaggcgc agcagcgcga ggtcgttcag gtcgtcgttg gtcagcggct cccccttgag
      661 gtgcgtgtgc accaggcgca ggccgcggaa gcggccgacg ccgccgcgca gacggccgac
      721 gtcggggagc gtcaggctgt gggcatcgcc gacgatgacg ttgcgcacct cgcccgcccg
      781 cgagagcagg acgccgactt ggcgccccag ccgctgcgag gtctcggtga ggtgccgcgc
      841 gagctcggga gagaccacct gccgcggatc gacgcggcgg cgataggtgc gctccagcgc
      901 ggagatgtcg gacgctttga tgcctcgggt atcaccggtg atctgagaga tgggaatgcc
      961 tccttagtgg accgtggggg gcgcgtagta ctcctcggga gccatcggct cgagccccat
     1021 acccattaac gcaagaagca gccgttctct ttcctccggc cccccggcct ccagcagcgc
     1081 ctgcttctcc gccggccgga acggcagcgc caccgacagg ccgttgagca gcgacacgcc
     1141 ggggatggcg gagaggaggt cgaggtcgaa gtccaggccg ttcgaggcgc cgaaggtgcg
     1201 cacggccgcc agcagccgcg agggatcgag ggcgctcgcc ggctcgttca ggtcggcggc
     1261 gaactcggcg gtgtcggcgc gcacccgccg gtagccgcgg tggagcggca gctcctcgcg
     1321 gatgcggaag cggcagatcc cccgcagcac gatctcgtag cggccgtccg gctgcggctc
     1381 gcagcgctcc atgcggccga ggcagcccac cgggtacagc tcggggttct ccggcggcag
     1441 ctccagcgcc ggccagttgt cctgccgcgg cacgaacggc tggatcatgc cgatggcgcg
     1501 ctccccctcc agcgcgtcgg ccaccatgtt gcggtagcgc ggctcgaaga tgttgagcgg
     1561 caggagcgtc cccggcagca gcagcgtgcc ggtgaggggg aagacgggaa tgacggcggg
     1621 caccgtgaaa tcagccatcg ctcgggctcc gtgcaatcgc cattcccgtc agcgtgccgc
     1681 cggaggggct atcgcccttc ggcgcaccag ttgagcacgg ccatggcgct cgtcatctta
     1741 tggtacgcct gccggaaggc gatgctctgc ggcccgtcga gcaccgcgtc gaggacctcg
     1801 tagttgcgca tggccggcag gtcgtggagg aagatggtgc cctgcttcga gacccgctcc
     1861 atcacctgcg gcgtcaccgt gtagggccgg aacttgtcga gccagtcctc gtccttcttc
     1921 tgcacgccca tggtcagcca gcgggtggtg tagaccgcgt cgacgtcctt ggggagcttg
     1981 tccaggtcgt ggtgctccac gatctcggag ccggtcttgg cggcgtagcg gagcgcccgg
     2041 tccatgtcct ccttgggcag gccgtagccc tcgggcgtca ccagcgtgag cttcatcttc
     2101 ggcagcatgc cgaacgcgag ggcgagcgcc gacgcggtgt tgttcccctc gcccatgaac
     2161 agcatgtgca cgccttcgag acgtcccagg gcctccttga gggcggccag gtcgcagatc
     2221 gcctgcgtcg ggtgctcgtt gtcgctcatc gcgttgacga tggacagccg gtcggaggcg
     2281 gcggcgagct gccgcatctc ttccatcggc tcgttggtgc ggaggaccag gatctccatg
     2341 tagttggcga gcacccgggc ggtgtcctcg atggtctcgc cggtgttgag ctggagatcg
     2401 ttcgggccgt aggcgatgac gtcggcgcct agcttcatcg ccccgacgtt gaacgaggtc
     2461 cgggtgcgcg tcgaggtctt cttgaagtag ataccgacga ccttgtcctg cagcggacgc
     2521 ttgccggccc actccccccg ggcaatggag acggcgtcat ccaccagccg ggacagggtg
     2581 tccgtatcca ggtccgcgat cgaaatcacg tgtcgaacag ccattccctc tccttgttca
     2641 tgaaagccta ccggtcgagt cctcgacccg tggggattcg ttcctctccc tagagtgccg
     2701 catgggggtc ggaacgggtc cgttccgccg ggcggagcgc gattcaggag tccgtgtcgg
     2761 acttcagcac cgcgaggaag gcctcctgcg ggatctccac cgcgcccacc atcttcatcc
     2821 gcttcttgcc ttccttctgt ttctccagga gcttgcgctt gcgggtgatg tcgccgccgt
     2881 agcacttggc ggtgacgtcc ttgcggaagg cgttgatcgt ggtccgggcg atgatggtgc
     2941 cgccgatcgc cccctggatc gcgatcttga actgttggcg cgggatggtg gcctccagcc
     3001 gctcgcagta gtggagggcc cgctgccgcg ccttctcgcg atggacgagc tgcgacaggg
     3061 catcgacccg ctcgccgttg accaggatgt cgaccttgac caggtcggtc tcgcgcaggt
     3121 cgagcatctc gtagtcgaat gagccgtacc cctgggtgat ggtcttcagg cggtcgtaga
     3181 agtcgaacag gatctcggcc agcggcagct cggagaccac ctgctggcgg ccgacggcga
     3241 ggaactgcac ctcgttgttg gtgccgcggc gctcgcggca gagctccatg accaccccca
     3301 ggtagcgctc ggggatcatg atcgccgccc ggatgtacgg ttcgcgcgcc ttttcgatgg
     3361 tccccgggtc ggggaacagc gccgggttgt cgatcatcac gatcttgtcg tcggcgaggg
     3421 tgatctcgta ctggaccgac ggcgcggtca ggaggagcga caggccatac tcgcgctcca
     3481 gccgctcctg cacgacctcc aggtggagga ggccgaggaa gccgcagcgg aagccgaagc
     3541 ccagggcgct cgaggagtcc ttctggaagg tgagcgccgc gtcgttgagc ttgagcttgt
     3601 cgagcgcctt ggcgaggtcc tcgtagtcgt cggtggcgat cggatagatc gatgagaaga
     3661 cgaccggctt ggcctccttg tagccgggga ggggctcggc ggccgggcgg tcggcttcgg
     3721 tgatggtgtc gccgacggtg atctcggaca ccgccttgac gccggcgatg acatagccga
     3781 cgtcgccggc cacgatctca tccgtcttga cccgcttgag gcgcagcagg ccgacctcct
     3841 ccacctggta gtccttgccg gtatggatga agcggatcgt ctgcttcggc cgcaccgtcc
     3901 cctccttcac ccggcagagg aggacgacgc cgcggtaggg gtcgtactgg gcgtcgaaga
     3961 tcagggcgcg cagcgggtcg gcggggtcgc cctggggggc cggcagcttg tggaccaccg
     4021 cttcgaggac gtcgtcgatg ccgatcccct gcttggcgga gcagagcacc gcttcgaacg
     4081 ggtcgagccc gaggtcggcg tcgatctcct cgcgcacccg gtcgacctcg gccgacggca
     4141 ggtcgatctt gttgatgacc ggcacgatct cgaggtcgta ctccagggcg agatagaggt
     4201 tggcgacggt ctgcgcctcg accccctggg aggcgtcgac gatcagcagc gccccctcgc
     4261 aggacatcag ggaccgccgc acctcgtggg agaagtcgac gtgccccggc gtgtcgatga
     4321 ggttcaggtg atagtcgcgg ccgtccttgg cggtgtagac gaggctcacc gtgttgcttt
     4381 tgatggtgat gccgcgctcc cgctcgatgt ccatcgaatc gaggatctgg tcgcggaaat
     4441 cgcgcagctc caccgcgcca cagcgctgga tgaggcggtc ggccagggtc gacttgccgt
     4501 ggtcgatatg ggcgatgatg cagaagttgc gaatgttgtc caaacctgaa tccctcggag
     4561 ttcgcgaagg gagatcctat accaaagggc cctccccccc ggtcccctct cccgcactgt
     4621 tacccatcct cccggtcgaa ccctctccgg ccgccatccc cgggagaggg ggaggtggac
     4681 ggaccctaac cagccgccct gccttggtcc ggagcgcctt gacgtctgcg aaccgcagac
     4741 tgaaggtggt ccaatgagat cgacgatcaa ccctccaaac gatctgtccg gccaggcaac
     4801 ccggctcgtc cctgaggaag ggggttctca ccgcctttga ggggagcggc cttggaacgg
     4861 cggcgccccg tcacgattca gttgtcaatg agcccggcgg ccggcggccc ccaggggcga
     4921 ccggcctgcc ctggcgagcg ctccactcgc ccttctgggc cggcctccgg ccccagctcc
     4981 cgccctgcag gacccggagg gccaaccggc cgccctcgag ggcatccctt ccgccccctg
     5041 gggcctggct gtcgccccat gggtcagcgg gtgggcccca cgggcatcca gcaggccccg
     5101 gagggctgac tccgcgcccc ttggggcatt ctgtgcgccc cctgctcttt gacaatcgaa
     5161 ggacacgagc cgggcgcggc gcccggatgg gaggcgccgc gcccgttcgt ggaggctcag
     5221 atgaagcttc ccgggaaggc tccattgatc ccgaaagaaa aaagccgccg ccctcgcggg
     5281 cggcggctcc ttgatgcacg gtacggctag ggcttacgcg ccgagggctt cgagaatccc
     5341 ctcggccgcg tcggtcagcg ccgcggcctg gtcggccgtg aggaggcctt ccgcggcgaa
     5401 gtcctccacc tgattgatga aggcgttgac ctggttgatg gccggtgtgg tgttgccgcg
     5461 gccgacctgc tgcgcggcgg ccctgagctt ggcgaggagc gcgttgccct ggccgttgtt
     5521 caggactccc gtgtttacca ggttcgtcac gtcctgctgg agcagaacga tctgctcggc
     5581 cgccggccgc acctgccaga agaccaccga ggtccgggcc ggcacctgga aggtgcaaat
     5641 cgcggggtta taggtggcga ggcgcaccac cgcgtccgcc gagttggcga gcaccggatg
     5701 gagcgtcagg ttcgggcagg cgaagaccga ggcctgcacc gactgcgcct gatcgttggc
     5761 gttgatcacc gtcacgatca tcttgtggcg gcggtccacc gtgccgtcgg tgtccgtcag
     5821 cgtgaacgcg atgacgccgg ggatctggtt cgggccggtg ttggcgaacg ccagacggtc
     5881 ctcgatctgc tccgccgtgc gcagccggaa cagcggcgag ctccggcgga tcatcagcac
     5941 ctcgcggaag tggtggtagg cccgctggat gtcctccggg gcgggctgga gcgccggatt
     6001 ggccagcagc ggcgccatga ccggccagtt gttctggttg tccgcggccg gcggcaggcc
     6061 gacgccgaaa ttgttgtcct gataggtgaa gtccagccgg ttgaaccagt cgccggagtt
     6121 gtagctgttg cggtccagcg acttcgagcg cagcagctcg acgccggcgt ggaagaacgg
     6181 tatcccctgc ccgaaggcca ggaggtccat tcccaggttc tgcatccgca cccggtccgc
     6241 catcgtcgcg gtcaccggcg ccttgagctg gatgatgtcg aacagcgtct cgttgtcgtg
     6301 cgcctcgacg tagttgatga tctcctgcgg gtcggccgtg tagccggtgg gctggccgtt
     6361 gtagtcgatc tcgcgcgcct cgaccaggtt gccctggctg tcgaccagct cgtactcggc
     6421 gaggccgccg gccatgccgg agcggatcca gtcggtgcgg tggagcagcg ccgcgagctg
     6481 atcggacgcc gatccctggt cggtgctgtt cgggtcgtaa tagaggccgg tcaggaagcc
     6541 ctgctcctgc aggccgctga acgggccgcc gccgcgggcg ccgtcgcgca gccggtcgtt
     6601 gaaggtgccg atgccggtgc cggccatgtt gatctgcgtc gcctgctcgc cgcgggcgtt
     6661 gttggccacc tcgccgaagt tccagccctc gccgtagacg tagatcttcc tgccgtccac
     6721 gccgtcggcg gccagggtga gggcgtccag ggctgcgcgc accgccagca tgttgcgctt
     6781 catgtggtgg cccatgatgt cgaaacggaa gccgtcgacc ttgtaggcct tcgcccaggt
     6841 caccaccgag tcgaccatga gcttctccat catgtcgtgc tcggtcgcgg tgttctggca
     6901 gcaggtgctg gtctcgacgt tgccgtcggc gttcaggcgg tggtagtagc cgggcacgat
     6961 gcggtcgagc accgacttgt cgttctggcc ggcggcgttg gtgtggttgt agaccacgtc
     7021 catgaccacc cgcaggccga tgccggagag gccggccacc atctcgcgga actcgcggac
     7081 gcgcgctggg ccgtcggggt cggtggagta gctcccctcg ggcacggtgt agtgccaggg
     7141 gtcgtagccc cagttgaagg cgtcgcggtc ggccaccgcc atcaccgccg cctgctgctg
     7201 ctcggagtcg gggggatagc tcgccaggtc accggccggg gagagccagg tggacttgtc
     7261 ctcgtcgatg gtggcgatgt cgaagaccgg cagcaggtgg acgtgggtga ggccggcctg
     7321 cgccagggag gccaggtgcc gcatgccgtt ggtgccgggc agggtgaagg ccttgtaggt
     7381 gccccggaag accggcgaca cggtggcgtc gttggcgctg aagtcgcgca ggtgcagctc
     7441 gtagagggtg atgtcctcgg gcgcggcgag cggcggcttg accagcgcgt cccagcccgc
     7501 gggctcgagc gccgggtcgg cgaggctgat gatttggctc cgccggctgt tgatcgacag
     7561 gctcaccgag taggggtcgg tcaccaggtt ggtctcgacc tcgccggtgg cgcggacgaa
     7621 gacctccacc tcgtagaggt agtagcggcc gtaccaggag gcctcgccgg tgacgctcca
     7681 gacgcccgtc gccggatcga gggtcatcgg gacgacggtg gagggggtcg ccgggttgga
     7741 gttggcgaag acgtggagct tcaccgactg cgccgtcggt gcccagacgc gcagggtcgg
     7801 gacggtaccg gcgagggtgg cgccgagcgg gccgtcgtag gagtagaggt cgtcgagcac
     7861 gccggggatc tggagcgcgg tggcgtccac cagcgtcccg tcaccagtcg atgcggagac
     7921 ggcgatctgc cccttgagga tctccgccac ctcgtcgagg tgctccgccg ggatcttgaa
     7981 ggcggccgag ccggccaggt gcgggaactt ctcgagcacg gcggcgggga ggccggccgg
     8041 atggtggatc agttggatgc cggtgccgcc ctggactccg tccgaggtca gggtcatccc
     8101 cccctcgggc gcgtgatgga gggtcacctc cgagccggcg gggaccttcc aggcgatggt
     8161 gtcctgagtg acccaatggg cctgggcgcg ggtgatgttg cccctggggc cctgggagct
     8221 ggtgccgacg gtgagcacgt gcgaggtcgc atcataggtg aagaagacct cgacgcaggc
     8281 cgccggcacg ctgaacgcga tgttggcgcc gtcgcgggca ccgccggcgc cgtagttctc
     8341 gctccagctc tcgtcgatcg ccaccttgac ctcgtagttg ccgggcggga tcaggcgggt
     8401 cgagaaggtg tggaggccgt cgccgtccgg gtcctgcagc caggagcgca ggcagtccgg
     8461 ctgccagtcg ccggggcagc cgatctccga ctggaagctg cccgccgcgg tggcgatgac
     8521 cgcgttgcgg ttgctggtca cccagtgggt gtcgtgggaa tagtagaact tgacggccgc
     8581 cgggtcggag aggctcaggc cgatgttcgg gccgttgagc gtggcgttgg cgccgtagtt
     8641 cacgtcccag gagccgtcga gcgccgcctt gtactcccag tcgccggccg gcaggtcgaa
     8701 cgtcccctgc cagacgccgt cggcggcgtc gtaggtcagg ccggtctcgg tgcagtcggg
     8761 ctgccagtcg ccggagcagc cg
//
LOCUS       DASPJI010000769         2435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_396724, whole genome shotgun sequence.
ACCESSION   DASPJI010000769 DASPJI010000000
VERSION     DASPJI010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396724"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VEL74_21495"
     CDS             <1..252
                     /locus_tag="VEL74_21495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65170.1"
                     /translation="MMVIPSAVNAGKAILGSGAHSVTAQWLAFLPGYLVLSAVTAWAL
                     YAAVERPVLLWRDRLLLRRRAAALPSASTAPAPEAAPSL"
     gene            complement(679..1089)
                     /locus_tag="VEL74_21500"
     CDS             complement(679..1089)
                     /locus_tag="VEL74_21500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019591846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG65171.1"
                     /translation="MKPEILQASRTCVLDASALLAVLHDEPGGDLVESLLETAVVSSV
                     NWSEVLQKALERQTDLRGLREDLESLGLEILAFTAAQAERAAQLRSTTRHLGLSLADR
                     ACLALAAELELPAITTDRVWQDLALDIEIRLARP"
     gene            complement(1086..1352)
                     /locus_tag="VEL74_21505"
     CDS             complement(1086..1352)
                     /locus_tag="VEL74_21505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HYG65172.1"
                     /translation="MSNSSALVKVSVGPQGRLVVPAEIRREMGIAPGDVLLARVEDER
                     LVLEKREVVLRRIRQRFAHIPKEVRLSDELIAERHSEAGHEDRG"
     gene            1532..>2435
                     /locus_tag="VEL74_21510"
     CDS             1532..>2435
                     /locus_tag="VEL74_21510"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HYG65173.1"
                     /translation="MSLVERLSERQLLVVTGKGGVGKTAVAATLGRLLSGVGRRALVV
                     EVDPRENLHQMFGVPPSGGDIVPVEPRLWVQNFKPRQVLDELVREQLKLELLSRRVLD
                     SPVYEHFAAGAPGLKEMAVLGHAYRMLHGQGGPGLPQVDLVVLDAPATGHGVTLLSAP
                     QLVSEVVKGGPFARMAADLARFVADPGRCGIVVVTQAEDMPVEESLELRRALRERLGR
                     EPEALVVNGLYPPLTGLAPEKDNELTSLWRRRRQLNERELARLAAEWEGPRVELPQLA
                     LDRGPALIATLGRCFGESTRQGGPW"
BASE COUNT          362 a          814 c          861 g          398 t
ORIGIN      
        1 atgatggtga tcccctccgc cgtgaacgcc ggcaaggcga ttctcgggag cggcgcgcac
       61 tccgtgactg cgcaatggct ggcgtttctt ccggggtacc tggtgctgtc cgccgtgacg
      121 gcgtgggcgc tctacgccgc cgtcgagcgg ccggtgctgc tctggcgcga ccgcctcctg
      181 ctgcgccgcc gtgccgcggc cctgccgagc gcctccacgg cgcccgctcc cgaagcagcc
      241 ccgtccttat agagatagag aggagccggc ggggtccctc ttccgccggg gaagactgcg
      301 gctgctgaaa acgtcgcggt gccgcgtttc atcttgcggc gaaggcttcg aagtcgcggt
      361 gcagcgttcc agcttgcggt gagccctccg aagttgcggt gaaccatctg agcttgcggc
      421 gaaccgtttg atcttgcggt gaaccggccc acatcgcggc gaacgaaacg aagtcgcggt
      481 gaatcttttc aagttgcggc gaatctctcg agcttgcggt gaaccgattc gagtcgcggc
      541 gtaccgaacg gagtcgcggt gaaggagttg aagtcgcggt ggagggatcg aagtcgcggc
      601 agacggatgg acgtcgcggt gaacggattg gggtcgcggc gccgattcct ggtagaccca
      661 gcgccgatcc ccggcggcct aggggcgggc aaggcggatc tcaatgtcca aggcgagatc
      721 ctgccacact cggtcagtgg tgatggcggg cagctccagc tctgcggcca acgcgagaca
      781 cgcccggtcg gctagcgaca gccccaagtg gcgggtcgtc gagcggagct gggctgcccg
      841 ctccgcttga gccgctgtaa aggcgaggat ctccaggccc agggactcga gatcctcgcg
      901 gaggcctcga agatctgttt gccgttccag cgccttctgc aggacctccg accagttgac
      961 gctggatacg acggcagttt cgagaaggct ctcgaccaga tcccccccgg gctcgtcatg
     1021 gagcacggcg agtagcgcgg aggcgtcgag cacacaagtc cggctggcct gcaggatctc
     1081 cggcttcatc ctcgatcctc gtgtccagct tcggagtgcc gctcggcgat cagctcgtcg
     1141 gagagccgca cctccttcgg gatgtgggcg aaccgctgcc ggatccttcg taggaccacc
     1201 tcccgctttt cgagaacgag acgttcgtct tcgacgcgag ccaacagcac atcgcctggg
     1261 gcgatgccca tctcacggcg aatttcagcc ggaacaacca gccgtccctg aggaccgacg
     1321 gagaccttga ccaatgcgct ggagttgctc atgtgcggat tgtgccacag ctctcggagg
     1381 tgtgccacgc ctcaccaggc gtggcagatc ccctggccta cgtgcgatag acggtcaccg
     1441 tccggcctct cggatatagt gttgccgcag gccggcaacc atcttgcaga ccggcgtgtg
     1501 gcgcgcgagc gtggaagcgg accctgatct catgagcctc gtcgaacgac tgtcagaacg
     1561 ccagctcctc gtggtcaccg gcaagggggg cgtgggcaag accgcggtgg cggccactct
     1621 cggccggctg ctgtccggcg tgggccggcg cgccctggtg gtggaggtgg acccgcggga
     1681 gaacctccat cagatgttcg gggtgccgcc gtcggggggc gacatcgtgc ccgtcgagcc
     1741 gcggctctgg gtgcagaact tcaagccccg gcaggtgctg gacgagttgg tccgcgagca
     1801 gctcaagctg gagctgctct cccggcgcgt cctcgacagc cccgtctacg agcacttcgc
     1861 cgccggcgcg cccggcctca aggagatggc ggtcctcggc catgcctacc gcatgctgca
     1921 cggccagggc ggaccgggcc tcccccaggt ggacctggtg gttctcgacg ccccggccac
     1981 cggccacggg gtgacgctgc tctccgcgcc gcaactggtg tcggaggtgg tcaagggggg
     2041 gccgttcgcc cgcatggcgg cggacctcgc ccgcttcgtc gccgatcccg ggcgctgcgg
     2101 catcgtggtg gtgacccagg ccgaggacat gccggtggag gagtcgctgg agctccgccg
     2161 cgcgctgcgg gagcggctgg ggcgcgagcc cgaggcgctg gtggtcaacg gcctctatcc
     2221 gccgctgacc ggcctggcgc cggagaagga caacgagctc acctccctct ggcgccgccg
     2281 ccgccagctc aacgagcggg agctggcgcg cctcgccgcc gagtgggagg ggccgcgcgt
     2341 cgagctgccg cagctcgctc tcgaccgcgg cccggcgctg atcgccacgc tcgggcgctg
     2401 cttcggcgaa tccacccggc aggggggccc atgga
//
LOCUS       DASPJI010000770         5816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_852704, whole genome shotgun sequence.
ACCESSION   DASPJI010000770 DASPJI010000000
VERSION     DASPJI010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5816)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852704"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VEL74_21515"
     CDS             complement(<1..561)
                     /locus_tag="VEL74_21515"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HYG65174.1"
                     /translation="MKPRTLLILLAVVLGLGAFIWFYERDLPSSEEREEQSRKVLAVE
                     KDDVRAVTLETAAGTVHLERVDPPTPPAEKTEKEEEEDGDEAAGAGTEALVESEWRIT
                     KPYTARAELMAVDRLLDELINLEKSRTLEDAVPADVGLDKPQATVRLATTEGEKVLKI
                     GAAVPTGGALIVGLEGSASAYVVGDSF"
     gene            complement(566..2023)
                     /locus_tag="VEL74_21520"
     CDS             complement(566..2023)
                     /locus_tag="VEL74_21520"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HYG65175.1"
                     /translation="MAETTAMNRRRLIHRGTLSAAALLAAALLFLVNYLGWKYHERFD
                     WTESSLYSLSEKSTNVLRDLDRDVRFVVFIPPNQQQELYDPVRELLARYEAASPRIKV
                     EFVDPEKNPVKAQQLAQQYGVTSPGVAVSSGDDRRFIDSADLVEVDFAAMQSGQQPGV
                     SGFKGEQLFTSTLVQLREGRKPRILFTTGHGERSLDDRGPQGFAGAQEILGRDNFEIE
                     EWASLGKPAVPEGTDLVVIAGPTGTFVQPELAALSAYLDRGGRVLVLLDPTLGQAAGS
                     GLLQTGLEAWLAGRGVQVGQNIVVDPSNPLPFFGPETIFARNYGSHPITEALAEGDLP
                     VLVSLVRSVGAAEGAPGATVLVSTSDEGWGENNLAQLEDVRRDGTDAAGPVPLGVAVG
                     GGEGASRLVVFGDSDFANNQLLEANLGNSVLLSNTLNWMVERQSLLGIPPKKTEQVRL
                     SLEQGQLWRLYALALLALPGLGVVLGSFIYFRRRR"
     gene            complement(2029..2769)
                     /locus_tag="VEL74_21525"
     CDS             complement(2029..2769)
                     /locus_tag="VEL74_21525"
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HYG65176.1"
                     /translation="MTAVWATFTRELRAYFFSPLAYVVLFFFLVVNGFIFSTIVTALA
                     DPRFGGGRPMDFFFGGSIFFWLILLFLTPVLTMRLLSEELRSGSIEVLMTAPVTEGQV
                     VVGKYLASLVFYLFLWLPTVAYAGILAYYSEVDWGPIASGYLGIVGIGALFLAIGLFA
                     SAMTRSQLLAAIISFALVMFLFIFGLLEGIVNNETARQVLGYVNLINHMDDFTKGIVD
                     TRRLVYYLSGILFFLFLTSRALEDRKWR"
     gene            complement(2766..3779)
                     /locus_tag="VEL74_21530"
     CDS             complement(2766..3779)
                     /locus_tag="VEL74_21530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYG65177.1"
                     /translation="MIEAQNLVRRYGDFTAVDGVSFAVQEGEIIGILGPNGAGKTTTI
                     RMITGFLPPTSGRVTVAGKDLLESPRQARREVGYLPENVALYTEMRVSEYLAYRARLE
                     GLRRAEAREAIGSALERCLLDDVRDQIIGTLSKGYRQRVGLATAILHRPRVLVLDEPT
                     VGLDPKQIIAIRELIRQLGRERTLLLSTHILPEVELLCDRVMIIDRGRIVAEGTPESL
                     RQSWIGNPALRLTLKEDPAGAGDVLASVPGVVAVRPGAAGPGNWTLECDRDADPREEV
                     FRAVVQSGWVLLELAAERTTLEDIFVRLTTHDTTHDRAGHQVNEPGEPPTSAPMSSEE
                     VVS"
     gene            4008..4286
                     /locus_tag="VEL74_21535"
     CDS             4008..4286
                     /locus_tag="VEL74_21535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65178.1"
                     /translation="MKSAYELALERLEKQGIERPREEGLTEEKREQIAEIRRQAEAKL
                     AELEILHRNRLKTVHDPVKRQEDEEEYVRERRRIEDDRERKVARLREG"
     gene            complement(4571..4930)
                     /locus_tag="VEL74_21540"
     CDS             complement(4571..4930)
                     /locus_tag="VEL74_21540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HYG65179.1"
                     /translation="MRVPPEEFEALVEQALDGLPEDFADLLENVVVMVEEEPDPEDLD
                     ALGLDPGEELFGLYQGVHQDERSTYYMGLPDRVVIYRGPILRACDNRRQVIREVRDTV
                     VHELGHHFGLDEEDMPY"
     gene            4979..>5816
                     /locus_tag="VEL74_21545"
     CDS             4979..>5816
                     /locus_tag="VEL74_21545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65180.1"
                     /translation="MSRLRALRSPPAALLGAVLVLAALHMLVFDRGLGGDGWAPFATL
                     ESLVDDGDLWLEDDHRGVMNGLVGSPGGHLVSQYPPGILVLDAVPFFAGRALDALLPE
                     GLLAGGVELPPAGRVPRGVFFSAAAIVLARNAATLLGLLWTGLALRRIGVPGGRAAAA
                     VALTFFGGPLLFYSLVGMTHAPTFALAALLLLLLVRWRAAPSLRLALAAGVVVGGAVL
                     VRYSAIALLPAAALALLPCSRERRSWSGLPAFAAGFAVPLLPLPFWWHASFGSWTPPP
                     YGG"
BASE COUNT          827 a         2001 c         2104 g          884 t
ORIGIN      
        1 gaaagagtcc cccacgacgt aggcgctcgc gctcccctcg agccccacga tcagcgcgcc
       61 gccggtcggg acggcggcgc cgatcttgag caccttctcc ccctcggtgg tggccaggcg
      121 caccgtcgcc tgcggcttgt cgagccccac gtcggccggt acggcgtcct ccagggtgcg
      181 gctcttctcg aggttgatga gctcgtcgag cagccggtcg acggccatga gctcggcacg
      241 ggcggtgtac ggcttggtta tccgccactc gctctcgacg agggcctcgg tgccggcccc
      301 cgccgcctcg tctccgtcct cctcctcttc cttctccgtc ttctcggccg gaggggtggg
      361 agggtccacc cgttccagat ggacggtgcc cgcggcggtc tccagggtca ccgcccggac
      421 gtcgtccttc tcgaccgcca ggaccttgcg ggactgctcc tcgcgctcct cgctcgacgg
      481 caggtcgcgc tcgtagaacc agatgaaggc gccgagcccg aggaccacgg ccagcaggat
      541 gagcagcgtg cgcggcttca cggcctcacc tccgccgccg gaagtagatg aaggagccga
      601 ggaccacccc gagccccggg agggccagga gcgccagcgc gtagaggcgc cagagctggc
      661 cctgctccag ggagagccgc acctgctccg tcttcttcgg cgggatgccc aggagcgact
      721 gccgctccac catccagttg agggtgttgg acaggaggac ggagttgccc aggttggcct
      781 ccaggagctg gttgttggcg aagtccgaat cgccgaagac caccaggcgc gacgcgcctt
      841 cccctccccc gacggccacc cccaggggca ccgggccggc ggcgtcggtg ccgtcgcgcc
      901 ggacgtcctc gagctgcgcc aggttgttct ccccccaccc ctcgtcgctg gtgctcacca
      961 ggaccgtggc ccccggggcc ccctcggccg cgccgacgga gcgaacgagg ctgaccagca
     1021 ccggcaggtc gccctcggcc agcgcctcgg tgatcgggtg gctgccgtag ttgcgggcga
     1081 agatggtctc cgggccgaag aacggcagcg ggttcgacgg gtcgacgacg atgttctggc
     1141 ctacctgaac gccgcggccg gcgagccagg cctcgaggcc ggtctggagg aggcccgagc
     1201 cggcggcctg ccccagggtg gggtcgagga gcacgagcac gcgcccgccg cggtcgaggt
     1261 aggccgacag cgcggcgagc tcgggctgga cgaaggtgcc cgtggggccg gcgatgacca
     1321 ccaggtcggt gccctcgggc accgccggct tgcccaaaga ggcccactcc tcgatctcga
     1381 agttgtcgcg gccgaggatc tcctgggcgc cggcgaaccc ctgcggcccg cggtcgtcga
     1441 gcgagcgctc gccgtggccg gtggtgaaaa ggatcctcgg cttcctcccc tcgcgcagct
     1501 ggacgagggt actggtgaag agctgctccc ccttgaagcc gctcaccccg ggctgctgcc
     1561 cgctctgcat ggcggcgaag tccacctcca ccaggtcggc gctgtcgatg aaccggcggt
     1621 cgtcgccgct cgacaccgcc acgcccgggc tggtgacgcc gtactgctgg gcgagctgct
     1681 gcgccttgac cgggttcttc tcggggtcga cgaactccac cttgatccgc ggcgaggcgg
     1741 cctcgtagcg ggcgagcagc tcgcgcaccg gatcgtagag ctcctgctgc tggttcggcg
     1801 ggatgaagac gacgaagcgg acgtcgcggt cgaggtcgcg caggacgttg gtcgacttct
     1861 cggacaggct gtagaggctg ctctccgtcc agtcgaagcg ctcgtggtac ttccagccca
     1921 ggtagttgac caggaagagc agggcggcgg ccaggagggc cgctgccgac agggtcccgc
     1981 ggtggatgag ccggcggcgg ttcattgcgg tcgtttcggc catgggcgtc acctccactt
     2041 ccggtcttcg agcgcgcggc tcgtcaagaa gaggaagaac aggatgccgg acaggtagta
     2101 gacgagccgg cgggtgtcga cgatcccctt ggtgaagtcg tccatgtggt tgatcaggtt
     2161 gacgtacccc agcacctgcc gggcggtctc gttgttcacg atcccttcga gcaggccgaa
     2221 gatgaagagg aacatcacca gcgcgaagga gatgatggcg gcgaggagct ggctgcgggt
     2281 catcgccgag gcgaagaggc cgatggccag gaacagcgcg ccgatcccga cgatgcccag
     2341 gtacccggag gcgatcggcc cccagtccac ctcgctgtag taggcgagga tgccggcgta
     2401 ggccacggtc gggagccaga ggaacaggta gaagacgagg gaggccaggt acttgcccac
     2461 caccacctgc ccctcggtga ccggggcggt catcagcacc tcgatcgagc cgctgcgcag
     2521 ctcctcggac agcaggcgca tggtgagcac cggcgtcagg aagagcagga tcagccagaa
     2581 gaagatcgag ccgccgaaga agaagtccat cggccggccg ccgccgaagc gcgggtcggc
     2641 gagggcggtg acgatggtgg agaagatgaa gccgttgacc accaggaaga agaacagcac
     2701 cacgtaggcg agcggcgaga agaagtaggc gcgcagctcg cgggtgaagg tcgcccagac
     2761 ggccgtcatg acaccacctc ctcggaggac atgggagcgg atgtgggggg ttcgccgggc
     2821 tcgttcacct ggtggccggc ccggtcgtgg gtggtgtcgt gggtggtgag gcggacgaag
     2881 atgtcttcga gggtggtgcg ctcggccgcc agctccagca ggacccagcc cgactggacc
     2941 acggcccgga agacctcctc gcgggggtcg gcgtcgcggt cgcactccag cgtccagttg
     3001 ccggggccgg cggcgcccgg ccgcaccgcg acgacccccg gcacgctcgc caagacgtcg
     3061 cccgcgccgg cggggtcctc cttcagggtg agccgcagcg cggggttgcc gatccagctc
     3121 tggcgcagcg actcgggcgt cccctcggcg acgatcctgc cgcggtcgat gatcatcacc
     3181 cggtcgcaga gcagctcgac ctcggggagg atatgggtgg acagcagcag cgtccgctcg
     3241 cggccgagct ggcggatcag ctcgcggatg gcgatgatct gcttggggtc gaggccgacc
     3301 gtcggctcgt cgaggacgag gacgcgcggg cggtggagga tggcggtggc gagccccacc
     3361 cgctggcgat agcccttgga gagggtgccg atgatctggt cgcgcacgtc gtcgagcagg
     3421 caccgctcca gggcgctgcc gatggcctcg cgggcctcgg cgcggcggag cccttcgagg
     3481 cgggcgcggt aggcgaggta ctcgctcacc cgcatctcgg tgtagagcgc cacgttctcg
     3541 gggaggtacc ccacctcgcg gcgcgcctgg cggggggact ccaacaggtc cttgccggcg
     3601 acggtcaccc ggccgctggt cggggggaga aagccggtga tcatgcggat cgtggtggtc
     3661 ttgcccgcgc cgttggggcc gagaatgccg atgatctcgc cctcctggac ggcgaaggag
     3721 acgccgtcca cggcggtgaa gtcgccgtag cggcggacca ggttctgcgc ctcgatcatg
     3781 gagcgtctgc ctcctcgtga gcttgcttcc gcgcttggga tggcccgggg gctcccggga
     3841 ccctcggttt tagcaaaacg gatgccctgc tgggaagagc cgtaggtcgc gatggatcaa
     3901 cgagttccat ctccggctca agaacgggcc acgtatggtc acgccattat ggcacgccag
     3961 ggcctcttct ctgctatctt caccgcatgg cagacaagac atccggcatg aagtccgcgt
     4021 acgagctggc gctcgagcgc ctggagaagc aggggatcga gcggccgcgc gaggaggggc
     4081 tgaccgagga gaagcgcgag cagatcgccg agatccgccg ccaggccgag gcgaagctcg
     4141 ccgagctcga gatcctccac cgcaaccgcc tgaagacggt ccacgacccc gtcaagcgcc
     4201 aggaggacga ggaggagtac gtccgcgagc gccgccgcat cgaggacgac cgcgaacgca
     4261 aggtagcccg gctgcgcgag gggtaggggg aagccgtccc agcctcgcga ccctcgtccg
     4321 atccttcatg cccctcgggt cgcacctggt tcgaacaggg tcgcgcctac ttcgaacagg
     4381 gtcgagccta ctttgaagag ggtcgagagc cttgacccgc gggtcgagac gactttgaag
     4441 cgggtcgaga gcctcgaccc tggggtcgcg cctactttga aacgggtcga aaggctcgac
     4501 cctcgggtcg gcacggctgc ggacggggtc gagacggctt cgcggcggcg cggccgtgcc
     4561 cgcgccggtc ctagtacggc atgtcctctt cgtccagccc gaagtggtgg ccgagctcgt
     4621 ggaccacggt gtcgcggacc tcgcggatga cctggcggcg gttgtcgcag gcgcggagga
     4681 tggggccgcg gtagatcacc acgcggtcgg gcaggcccat gtaataggtg ctcctctcgt
     4741 cctggtggac gccctggtag aggccgaaca gctcctcgcc gggatcgagc cccagggcgt
     4801 cgaggtcctc ggggtcgggc tcctcctcga ccatgacgac cacgttctcg agcaggtcgg
     4861 cgaagtcctc gggcaggccg tccagggcct gctcgaccag ggcctcgaac tcttcggggg
     4921 ggacgcgcat ggggggagag tttagcctcc ctccttataa taatgccgca tcccctccat
     4981 gtcccgactc cgcgccctcc gctcaccccc cgccgccctg ctcggcgccg tgctcgtcct
     5041 ggcggccctg cacatgctgg tgttcgaccg cgggctcggc ggcgacggct gggccccctt
     5101 cgccaccctg gagtcgctgg tggacgacgg cgacctctgg ctcgaggacg accaccgggg
     5161 ggtgatgaac ggcctcgttg gatcccccgg gggccacctc gtctcccagt acccgcccgg
     5221 gatcctggtc ctcgacgccg tgccgttctt cgccggccgg gcgttggacg cgctcctccc
     5281 cgaggggctc ctggccggcg gagtggagct gccgccggcc ggcagggtcc cccgcggcgt
     5341 gttcttctcg gccgccgcca tcgtgctggc gcgcaacgcc gcgacgctcc tcgggctcct
     5401 ctggaccggc ctcgccctgc gccggatcgg cgtgcccggg ggccgggccg cggcggccgt
     5461 ggcgctcacc ttcttcggcg gcccgctgct gttctactcg ctggtgggga tgacccacgc
     5521 cccgaccttc gcgctggcgg ccctgctcct cctgctgctc gtgcgctggc gggcggcgcc
     5581 gagcctgcgg ctcgccctgg cggcgggcgt ggtggtcggc ggcgcggtgc tggtgcgcta
     5641 cagcgccatc gccctgctcc cggcggcggc gctggccctg ctcccctgct ctcgggagag
     5701 gcggtcctgg tccggcctgc ccgccttcgc ggcggggttt gccgtgccgc tcctccccct
     5761 ccccttctgg tggcacgcga gcttcggctc ctggacgcct cccccctatg gcggcc
//
LOCUS       DASPJI010000771        11574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_272812, whole genome shotgun sequence.
ACCESSION   DASPJI010000771 DASPJI010000000
VERSION     DASPJI010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11574)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11574
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272812"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1010
                     /locus_tag="VEL74_21550"
     CDS             90..1010
                     /locus_tag="VEL74_21550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HYG65181.1"
                     /translation="MHTYSIVARDPATGELGVAVQSHWFSVGSIVTWAEAGVGAVATQ
                     SFVRVDYGPLGLNLMKEGKAAQEALDQLLAADPQKDVRQVAFVDAQGRVASWTGPKCI
                     QAAGHQTGKAFSVQANLMDKPAVWPAMARAFESAKGDLAERLLAALEAAEAEGGDIRG
                     KQSAALIVVRAKSTGEPWRDRLVDLRVDDNPAPLVELRRLLKLHRAYEQMNLGDEAAA
                     LEKWDAALGHYTAASKMAPEIAELPFWQAVTLFMAGQEDKALPVFREVFAREERWARL
                     VPRLPAAGLLPDDPKKIEKILSVAPKSNRQ"
     gene            complement(1011..1976)
                     /locus_tag="VEL74_21555"
     CDS             complement(1011..1976)
                     /locus_tag="VEL74_21555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated
                     beta-propeller protein"
                     /protein_id="HYG65182.1"
                     /translation="MTIWKAFLVLLLASYPAAAAPLAYVSNERAGTVTVIDTATQKGL
                     YTLRVGHRPRGIQVSPDGSRVYVALSDTARNDQGDGDAIVAFDPKRPRIVARYDGGTD
                     PEQFAVSPDSKRLYSSNEDAGTASVTDATTKKVIASLIVGIEPEGVAISPDGRWVYVT
                     AETSNTVSVIDTKKNEVVASFLVDPRPRAAAFSPDGRRAYVTAEIGGSVSVVDVARHE
                     VIKTIDLPGARPVGVVVSPDSKLVYVANGHGNSVAVIDAAASRLVATIPVGRRPWGIA
                     ITPDGRKVYTANGVSNDVSVIDTATRKVVATIKAGAGAWGVAIGR"
     gene            complement(1973..2728)
                     /locus_tag="VEL74_21560"
     CDS             complement(1973..2728)
                     /locus_tag="VEL74_21560"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HYG65183.1"
                     /translation="MITRRTFLRSILGAGALAAGAGRLHAAPEPRLIRVGLILPKDGA
                     FAESLRRGAELGAQEAGRTAELLRARFELLTATAGDPQEASREATRLIEQSNVMALVG
                     GVEAAIRAALGEVADRHQVLLITTGSPSEAAEAPGRFRFHLAPRRAAGSRGMEWHHSL
                     SRFGAEQLNQRFESSFGKPMDSMAWMGWMAVRIPVELALRHPSATKAELPERLKAMRF
                     DGHKGARLEFDPSHHLRQPLYRVEGDKAVEEVP"
     gene            complement(2725..3108)
                     /locus_tag="VEL74_21565"
     CDS             complement(2725..3108)
                     /locus_tag="VEL74_21565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HYG65184.1"
                     /translation="MRLSTTRAALGTALLLIVFSGCGGDAAQEAAQRTGGDSQRGRQH
                     MLKYGCASCHVIPGVEGARGMVGPSLEGIASRTYLGGKLPNTPENMTLWVRDPQSVSP
                     GTAMPNLGVTEEDGRDMVAYLYTLK"
     gene            complement(3098..4027)
                     /locus_tag="VEL74_21570"
     CDS             complement(3098..4027)
                     /locus_tag="VEL74_21570"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HYG65185.1"
                     /translation="MIALLHGAEKHLTASEVLRWWTWDPLVGLCLGLSGVLYILGLQR
                     LWASAGADRGVRRWEAAAFAAGWLSLFVALVSPLDALGGVLFSAHMAQHEVLMLIAAP
                     LFVLGRPLVVFLWALPPRGREAVGRWTHRPWVSSGWRALSGPFVVWAIHGVALWVWHV
                     PRLYQATLTSDAVHAVQHASFFGSAALFWWALIHGRYGRFGYGVAVAYVFLTGMHSGV
                     LGALLTFAPSLWYPIYEARTGEWGLSALEDQQLAGLIMWVPAGAVFMLLGLALFAAWL
                     GEAERRVAFTESERILSVLETAALETERGIHEA"
     gene            complement(4024..4350)
                     /locus_tag="VEL74_21575"
     CDS             complement(4024..4350)
                     /locus_tag="VEL74_21575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65186.1"
                     /translation="MLWAGVMAGPLAWALHQQVSYMLVPTACAKGTEGMLHLVTLAAL
                     LMAAAGALISWRSWKRLPEGSTEMGDAQDTRRRFMALWGLVLSLAFSLVIVAGEIPNW
                     FLGACD"
     gene            complement(4398..5009)
                     /locus_tag="VEL74_21580"
     CDS             complement(4398..5009)
                     /locus_tag="VEL74_21580"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HYG65187.1"
                     /translation="MKQRPVIDVSELPSVVFGSRAPIWWGVVGLVTIESMGFALLAAA
                     YFYLRGGASEWPPPGVINPPLALTTTALLVLLASSFCMHEAAKAAKRAELGRILRWLI
                     ATAGLGYVFLVLRGFEFASMSYEWRSHAYGSIVWVIVGIHTLHILTSAVEKTIFIVLF
                     IRGPVEDKHLTDVATNEVYWHFVSLSWLVFYVILYLEPGFFRP"
     gene            complement(5006..6943)
                     /gene="ctaD"
                     /locus_tag="VEL74_21585"
     CDS             complement(5006..6943)
                     /gene="ctaD"
                     /locus_tag="VEL74_21585"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HYG65188.1"
                     /translation="MSPKSVVIQAEPETPEADLLDEVHHRLEGAWRPEKGFIGWLSEV
                     RHTAIAKRFIVTAFIFFALGGIEALLMRIQLSRPENGLLSPDLYNQLFTMHGTTMMFL
                     FAVPIMEAVGLYLVPLMIGTRELAFPRLINYSYWIYLIGGLLLYSGFFLSMAPDAGWF
                     SYVPLSGPEYSPGKRVDVWAQMITFTEVSGLATSVTLIVTIFKQRAPGMSLNRMPLFV
                     WTQLITSLMVVFAMPAVMVSSSLLAMDRLIDTHFFNPGEGGDAILWQHLFWFFGHPEV
                     YIIFLPATGFVSAIIVPFTRRPIFGYNAIVLSLVATSFIGFGLWVHHMFATGLPQLGQ
                     SFFTAASIMIAIPSGVQIFCWIATLWGGRIRFRTPMLFVLGFIAIFVAGGLTGVMLAA
                     VPLDLQVHDTFFVVAHFHYVLIGGAVFPLIGAVYFWFPKMTGRMMSERAGKWSFWLLF
                     AGFNLTFFPMYQLGLQGMPRRVYTYLGESGWTDLNRLATLGGLVLTAGVVVFILNALW
                     SLRHGAIAGDNPWDADTLEWETSSPPPSYGFLYLPTVHGRHPLWMPRENQPVVVGLQD
                     NRREVLVTHTLDAEPDHRDIVAGPSIWPFVLALVTGFTFITVIFTPWALPWGIVLAII
                     PLVCWFWPRSDMGRVILKEKA"
     gene            complement(6940..7947)
                     /gene="coxB"
                     /locus_tag="VEL74_21590"
     CDS             complement(6940..7947)
                     /gene="coxB"
                     /locus_tag="VEL74_21590"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HYG65189.1"
                     /translation="MNALAFLAGSGFAGRQSALNPAGPQAGRIEDLWWLMFWVCTAVF
                     VLVMLFFIAGMVRGRRARKERGVEAPMSVEPGVERRMMRTIVAAVAATVITLFGLLVA
                     SVATGRSISPLRVPEDALTVKVIGHQWWWDFEYQHPEPYRIIRSPNELHIPVGQPVRI
                     LTTSADVIHSFWVPNLHGKLDLIPGRTADTWIQADRPGVYRGQCAEFCGHQHAHMGFL
                     VIAEPPERFAAWWERQRQPAPEPATPLQQRGRQVFLSQACPLCHTIQGTDAGSRVGPD
                     LTHLASRRTLAAGTLPNTRGHLGGWIADPQSTKPGNLMPPNPMKSEDLQALLAYLESL
                     R"
     gene            complement(7944..8468)
                     /locus_tag="VEL74_21595"
     CDS             complement(7944..8468)
                     /locus_tag="VEL74_21595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HYG65190.1"
                     /translation="MTILAVAVLGGCEREMRPPEVLPSSAARPRPIVRQTTLQPGRPL
                     AQAEGRGPYDENAYSISQGQRLFSQYNCVGCHAHGGGGMGPALIDDTWIYGSEPQNIY
                     ATILEGRPNGMPSFGGHIPEQQAWQIVAYVRAMGRLVPKAAAPGRNDHLYSGESPQAS
                     GPGEELRQPAEHPR"
     gene            complement(8483..9286)
                     /locus_tag="VEL74_21600"
     CDS             complement(8483..9286)
                     /locus_tag="VEL74_21600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HYG65191.1"
                     /translation="MLLFFLIAALFISCAPAPPARTLRVCADPNNLPFSNRQLEGFEN
                     EIAKVIAREMGARVEYTWWAQRRGFFRNTLRAGLCDVVMGVPSSFELAMATRPYYRSS
                     YVFVYPKDSGITVRSFDDPALRRLRVGVQMIGDDLHNAPPAHALASRGVIDNVRGYSV
                     YGDYREPNPPARIVEAVAAGEIDVAVVWGPLAGYFARRQAVPPLEIVPVSPQIDLPFL
                     PFVYDISMGVRREDTALRDELDAILERRRPEIEAILDRYGVPRPERSRG"
     gene            complement(9492..11234)
                     /locus_tag="VEL74_21605"
     CDS             complement(9492..11234)
                     /locus_tag="VEL74_21605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019020678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HYG65192.1"
                     /translation="MGQGAGRISGKLSPADLQADDGQWVMAAKNYASTRFSGLGQINV
                     QSAKNLKVAWTFSTGSLRGHEAAPLVVGGTMYVVTPYPNQLYALDLTQPGAPAKWVYE
                     PQPDPAAQGVACCDVVNRGAAYANGRIFYNTLDNHTVAVDAETGKEVWKTKLGDINKG
                     ETMTMAPLVVKDKVLVGNSGGELGVRGWLTALDAGSGKIAWRAYSTGPDKDVLIGPEF
                     KPFYPMDQGTDLGVKSWPPDAWKMGGGTVWGWISYDPELDLIFYGTGNPGPWNPDQRP
                     GDNKWTCGVFARDPDTGQARWFYHVGPHDHFDYDGINESILLDLEVGGRKRKVLVRPE
                     RNGYVYVMDRATGQVLSANPFAPITVSTGVDLKTGRIQEVEAKKTGVGKVVREICPAS
                     PGAKDWQPSAFSPRTGLLYMPHNNLCMDYEGVEANYIAGTPFVGANVKMYAGPGGNRG
                     FFTAWDPVAGKPRWQIKEKFPAWSGALVTAGDVVFYGTMDGWFKALDARNGQELWKFK
                     TGSGIIGQPVTYRGPDGKQYVAILSGVGGWAGAVVAGDLDPRDGTAALGFANAMADLK
                     EATTRGGMLYVFSL"
BASE COUNT         2013 a         4030 c         3802 g         1729 t
ORIGIN      
        1 gatactgtat gtttactgtc atgctcaaga cgctcaccgc ggccgcgatg ctcctcgcgt
       61 gcgttcacgc ggcacaggcg gatccgctcg tgcacacgta ttccatcgtg gcgcgggatc
      121 ccgccaccgg cgagcttggc gtcgccgtgc agtcgcactg gttctcggtc ggatcgatcg
      181 tgacgtgggc cgaggcaggc gtcggcgccg tggccacgca gtcgttcgtg cgggtggact
      241 acggcccgct ggggctgaac ctgatgaagg agggcaaggc ggcgcaggag gccctggacc
      301 agctcctggc cgccgacccg cagaaggacg tgcgccaggt ggcgttcgtc gacgcccagg
      361 ggcgggtcgc gtcctggacc ggccccaagt gcatccaggc ggcgggccac cagaccggca
      421 aggcgttctc ggtacaggcc aacctcatgg acaagccggc ggtctggccg gccatggcgc
      481 gggcgttcga gtccgccaag ggggacctcg ccgagcggct cctcgccgcc ctcgaggccg
      541 ccgaggccga gggaggagac atccgcggca agcagagcgc cgcgctgatc gtcgtccggg
      601 ccaagtccac cggcgagccc tggcgcgacc ggctggtgga cctgcgggtg gacgacaacc
      661 ccgcgcccct ggtggagctg cggcgcctgc tcaagctcca tcgggcctac gagcagatga
      721 acctcggcga cgaggcggcg gctttggaga agtgggacgc ggctctcggc cactacaccg
      781 cagcgtcgaa gatggcgccc gagatcgccg agctcccctt ctggcaggcg gtcaccctgt
      841 tcatggccgg ccaggaggac aaggccctcc ccgtgttccg ggaggtcttt gcccgcgagg
      901 agcgctgggc gcggctggtg ccccggctgc ccgccgcggg cctgctgcct gacgatccca
      961 agaagatcga gaagattttg agcgtggccc cgaaatccaa ccggcagtga tcaccgtccg
     1021 atcgccactc cccaggcgcc ggcgccagcc ttgatcgtcg cgaccacctt gcgggttgcc
     1081 gtatcgatca ccgacacgtc gttgctcacg ccattggccg tatagacctt acggccatcc
     1141 ggcgtgatgg cgatccccca cgggcgtcgc cccacgggga tggtggcgac gagccggctc
     1201 gccgccgcgt cgattacggc cacggaattg ccgtggccgt tcgccacgta cacgagcttg
     1261 ctgtcgggcg agaccacgac gcccacgggc cgggcgccgg ggaggtcgat ggtcttgatc
     1321 acttcgtgcc gcgccacgtc caccaccgag accgagccgc cgatctcggc cgtcacatag
     1381 gcgcgccggc catcgggcga gaaggccgcg gcccgcggcc gggggtccac caggaagctc
     1441 gccaccacct cgttcttctt cgtatcgatc accgagaccg tgttgctcgt ctccgccgtg
     1501 acatagaccc accgtccgtc cgggctgatc gccactccct cgggctcgat ccccacgatg
     1561 agcgaagcga tcactttctt cgttgtcgcg tcggtgaccg aggccgtgcc cgcgtcctcg
     1621 ttcgagctgt agagccgctt gctgtccgga gagacggcga actgctcggg atcggtgccg
     1681 ccgtcgtagc gggcaacgat gcgagggcgc ttgggatcga aagcgacaat ggcgtcgccg
     1741 tccccctgat cgttgcgggc cgtgtcgctc agggccacgt agacgcgcga gccgtccggg
     1801 ctgacctgga tcccccgcgg ccggtgcccg acccgcaagg tgtagagccc cttctgggtg
     1861 gccgtatcga tcaccgtcac cgtgcctgcc ctctcgttgc tgacgtaggc gaggggggcc
     1921 gcggcggccg ggtaggacgc caagaggaga acgagaaaag ctttccaaat cgtcatggca
     1981 cctcctccac agccttgtcc ccctccactc tgtagagcgg ctggcgcaga tggtggctgg
     2041 gatcgaattc aagacgggct cccttgtggc cgtcgaagcg catcgccttc agccgctccg
     2101 gcagctcggc cttggtcgcg gaggggtggc gcagcgccag ctccaccggg atgcggaccg
     2161 ccatccagcc catccacgcc atggaatcca tgggcttccc aaagctggat tcgaaacgct
     2221 ggttgagctg ctccgccccg aatctcgaaa gagagtgatg ccactccatg ccacgcgagc
     2281 cggcggcgcg gcgcggcgcc aggtggaacc ggaagcgccc cggcgcctcc gccgcctcgg
     2341 agggcgatcc ggtcgtgatc agcagcacct gatgccggtc ggcaacctct cccaaggcgg
     2401 cgcggatggc ggcctcgacg cctcccacca gggccatcac attggattgc tcgatgagcc
     2461 gcgtggcctc ccgagaggcc tcctggggat cgccggccgt ggcggtcagg agctcgaagc
     2521 gggcgcggag cagctcggcg gtacggccag cctcctgagc gccgagctcg gcccctcgcc
     2581 gcagggactc cgcgaaggct ccatccttgg gcaggatcag ccccacgcgg atcagccgcg
     2641 gctccggggc ggcgtggagc cggcccgcgc cggcggcgag cgcgccggct ccgaggatcg
     2701 atcgcaggaa agttcggcgc gtgatcattt caaggtgtag agataggcga ccatgtcccg
     2761 cccgtcctcc tccgtcacgc cgaggttcgg catggcggtc cccggggaga cgctctgggg
     2821 gtcgcgaacc cacagcgtca tgttctccgg cgtgttcggc agcttgccgc ccaggtaggt
     2881 ccggctggcg atcccctcga gggaagggcc caccatgccg cgggccccct cgacgccggg
     2941 gatgacgtgg caggacgcgc agccatactt gagcatgtgc tgccggcccc gctgcgaatc
     3001 gccgccggtc ctctgcgcgg cctcctgcgc ggcgtccccg ccgcagccgg agaaaacgat
     3061 caggagcagt gccgttccca gcgccgctct cgtggtgcta agcctcatga atacccctct
     3121 ctgtttccag ggccgctgtc tccaagacag acaggatcct ctcgctctcg gtgaaggcga
     3181 cccgccgctc ggcctcgccg agccatgccg cgaacagggc gagccccagg agcatgaaga
     3241 ccgcccccgc cggcacccac atgatgagtc cggcgagttg ctggtcctcc agggccgaga
     3301 gcccccactc gcccgtccgc gcctcataga ttggatacca cagagagggg gcaaaggtca
     3361 ggagggcgcc caggacaccg ctgtgcatgc cggtaaggaa gacgtacgcc accgccaccc
     3421 catacccgaa ccggccgtag cgtccatgga tcagagccca ccagaagaga gccgcgctgc
     3481 cgaagaagct ggcgtgctgg accgcgtgga ccgcgtcgct ggtgagcgtc gcctggtaga
     3541 gccgcggcac gtgccacacc cagagcgcca cgccgtggat cgcccacacc acgaacgggc
     3601 cgctgagcgc ccgccatccc gagctcaccc agggccggtg cgtccatcgc cccaccgcct
     3661 cgcgtcctcg tggaggcaac gcccacagga acaccacgag aggccgcccc agcacgaaga
     3721 gaggcgcggc gatgagcatc agcacctcgt gctgggccat atgggccgag aacaggacgc
     3781 cccccagagc atcgagcggc gagaccagcg ccacgaacag cgacagccac cccgccgcga
     3841 acgccgccgc ctcccagcgg cgcacgccgc ggtccgcccc ggcgctcgcc cacagccgct
     3901 gcagccccag gatatagagg acacccgaga gccccaggca gagccccacg agcgggtccc
     3961 acgtccacca gcggagcact tcggaagcgg tgaggtgctt ctccgctcca tgaagcaatg
     4021 cgatcaatcg cacgccccca ggaaccagtt cgggatctcc cccgccacga tcaccaggct
     4081 gaaggccagg ctcagcacca ggccccagag cgccatgaaa cggcggcggg tgtcctgcgc
     4141 atcccccatc tccgtcgagc cctccggcag ccgcttccac gaccgccagg aaatcaacgc
     4201 gccggccgcg gccatgagga gcgccgccag cgtcacgaga tggagcatcc cctcggtgcc
     4261 tttcgcgcac gccgtcggca ccagcatgta gctcacctgc tgatggagcg cccaggcgag
     4321 cggaccggcc atcacgccgg cccaaagcac cagatttccg gactgccgat ggatcgcttt
     4381 cgtggtctcg gacattgcta cggccggaag aatccgggct ccagatagag gatcacgtag
     4441 aacaccagcc aggagaggga cacgaaatgc cagtagacct cgttggtcgc cacgtccgtc
     4501 aggtgcttgt cctccaccgg cccgcggatg aacaggacga tgaaaatcgt cttctccacc
     4561 gccgaggtca ggatgtggag cgtatgaatc cccacgatca cccagacgat ggagccgtac
     4621 gcgtggctcc tccattcata ggacatggag gcaaactcga aaccgcggag gaccaggaag
     4681 acgtagccga ggccggccgt cgcgatcagc cagcgcagga tgcgcccgag ctcggcccgc
     4741 ttcgccgcct tggccgcctc gtgcatgcag aacgagctcg ccaggaggac cagcagcgcc
     4801 gtggtggtca aagccagcgg tgggttgatc acgcccggcg gcggccactc gctcgctccg
     4861 ccccgcaggt agaaataggc cgcggcgagc aacgcgaacc ccatcgactc gatggtgacc
     4921 aggcccacca cgccccacca gatgggcgcg cggctgccga agaccacgga cggcagctcg
     4981 gagacatcga tcaccggccg ctgcttcatg ccttctcctt gaggatcacc cgtcccatgt
     5041 cgctccgcgg ccagaaccag cagaccaggg ggatgatcgc gagcacgatc ccccagggta
     5101 gcgcccacgg ggtgaagatg acggtgatga atgtaaagcc cgtcaccagc gcgagcacga
     5161 acggccagat cgaaggcccc gcgacgatat cccggtggtc cggctccgca tccaacgtgt
     5221 gggtcaccag cacctcgcgg cggttgtctt gcagccccac cacgacgggc tggttctcgc
     5281 gcggcatcca caggggatgg cgcccgtgca ccgtcggcag atagaggaat ccgtaggatg
     5341 gcggaggcga gctggtctcc cactcgagcg tgtcggcgtc ccacgggttg tcaccggcaa
     5401 tggcgccgtg ccgcaggctc cacagcgcgt tgagaatgaa caccacgacc cccgcggtga
     5461 gaacaaggcc accgagcgtg gccagccggt tcaggtcggt ccagccgctc tcgccgagat
     5521 aggtgtagac ccgccgcggc atgccctgga gcccgagctg gtacatggga aaaaaggtga
     5581 gattgaaccc cgcgaacagc agccagaagc tccacttccc cgcccgctcg gacatcatcc
     5641 gcccggtcat cttcgggaac cagaaataga ccgcgccgat caaagggaag accgcgccgc
     5701 cgatcagcac atagtgaaaa tgggccacca cgaagaacgt gtcgtggacc tggaggtcca
     5761 gcggcaccgc ggcgagcatc acgccggtca atccgccggc tacgaagatg gcgatgaagc
     5821 cgaggacgaa gagcatcggc gttcggaatc gtattcgccc gccccacaag gtggcgatcc
     5881 aacagaagat ctggaccccg ctcggaatcg cgatcatgat gctcgccgcc gtgaagaagc
     5941 tctgcccgag ctgcggcagg ccggtggcga acatgtgatg cacccacagg ccgaagccga
     6001 tgaagctcgt cgccaccagc gacaggacga tcgcgttgta gccgaagatc ggccggcggg
     6061 tgaacgggac gatgatcgcc gacacgaagc cggtggccgg caggaagatg atgtagacct
     6121 cggggtggcc gaagaaccag aacaggtgct gccagaggat ggcatcgccc ccctcgcccg
     6181 gattgaagaa gtgggtgtcg atcagccggt ccatggcgag cagcgagctg ctgaccatga
     6241 ccgccggcat ggcgaacacc accatgagcg aggtgatcaa ctgcgtccag acgaagagcg
     6301 gcatgcggtt gagcgacatg ccgggtgcgc gctgtttgaa gatggtaacg atcagggtga
     6361 ccgaggtggc gaggcccgag acctcggtga acgtgatcat ctgcgcccac acgtccaccc
     6421 gcttgccggg cgagtactcg gggccggaga gagggacata gctgaaccag cccgcatccg
     6481 gcgccatgct caggaagaaa ccgctgtaga gcagcagccc gccgatcaga tagatccagt
     6541 agctgtaatt gatcagccgc gggaacgcca gctcgcgcgt gccgatcatc agcggcacga
     6601 gatagagccc gacggcttcc atgatcggca ccgcgaacag gaacatcatg gtggtgccgt
     6661 gcatggtgaa gagctggttg taaaggtcgg ggctgaggag gccgttctcc ggccgggaga
     6721 gctggatgcg catgaggagc gcctcaatcc cccccagggc gaagaagatg aacgcggtga
     6781 cgatgaaccg tttggcgatc gcggtgtggc gcacctccga caaccagccg atgaaccctt
     6841 tctccgggcg ccaggcgcct tccaggcggt gatggacctc gtcgaggagg tcggcctcgg
     6901 gggtttcggg ctccgcctgg atgaccaccg acttcgggct catctcaggc tctccagata
     6961 agcgagcaag gcctgcagat cctccgattt catgggattg gggggcatca ggttccccgg
     7021 tttggtgctc tggggatcgg cgatccagcc gccgagatgg ccgcgggtgt tgggcagggt
     7081 cccggccgcg agggtgcggc ggctcgcgag gtgcgtcagg tcgggcccca cgcggctgcc
     7141 ggcgtcggtc ccctggatgg tgtggcagag cgggcaggcc tgggaaagga agacctgccg
     7201 tccgcgctgc tggagcggcg tggccggctc cggcgcgggc tggcgctgcc gctcccacca
     7261 ggcggcgaac cgctccggcg gctcggcgat caccaggaag cccatgtgcg cgtgctggtg
     7321 gccgcagaac tcggcgcact ggccgcggta gacgcccggc cggtcggcct ggatccaggt
     7381 gtccgccgtg cggcccggga tcaggtcgag cttgccgtgg aggttgggca cccagaagct
     7441 gtggatcacg tccgccgacg tggtcaggat ccgcaccggc tgcccgaccg ggatgtgcag
     7501 ctcgttgggt gagcggatga tccggtaggg ctccgggtgc tgatactcga agtcccacca
     7561 ccactggtga ccgatcactt tgacagttaa ggcatcctcg ggaacccgga gcggcgagat
     7621 cgaccggccg gtggcgaccg aggccacgag aagcccgaag agtgtgatca ccgtcgccgc
     7681 caccgccgcg acgatcgtcc gcatcatccg ccgctccacc ccgggctcga cggacatggg
     7741 cgcttcgacg ccccgctcct tgcgcgcccg cctcccgcgc accatgccgg cgatgaaaaa
     7801 gagcatgacg agcacgaaca cggccgtgca cacccagaac atcagccacc agaggtcttc
     7861 gatccgtccc gcctggggcc cggccgggtt gagcgccgac tggcggccgg cgaagccgga
     7921 gccggctaga aaggcgaggg cattcatcgt ggatgctccg ccggctgccg cagctcctcg
     7981 cccggcccgg acgcctgggg ggactcgcca gaatatagat gatcattccg ccccggcgcc
     8041 gccgccttgg gcaccagccg gcccatcgcg cgaacgtagg ccacgatctg ccacgcctgc
     8101 tgctccggga tgtgcccgcc gaacgacggc atcccgttgg gccgtccctc gaggatcgtc
     8161 gcgtagatgt tctggggctc gctgccgtag atccacgtgt catcgatcag cgccgggccc
     8221 atccccccgc cgccgtgcgc gtggcagccg acgcagttgt actggctgaa cagccgctgc
     8281 ccctgactga tcgaataggc gttctcgtcg tacggccccc tgccttcggc ctgcgccagc
     8341 ggcctgccgg gctggagcgt cgtctgccgc acgatcggtc ggggcctggc ggccgaggac
     8401 ggcagcacct ccggcggcct catctcgcgc tcgcaccctc cgaggacggc cacagcgagg
     8461 attgtcaaga tcccgtttct ggtcatccgc ggctcctttc tggacggggc acgccatagc
     8521 ggtcgaggat cgcctcgatc tccggccgac gccgctccag gatggcgtcg agctcgtcac
     8581 ggagcgccgt gtcctcgcgc cgcacaccca tggagatgtc atagacgaac ggcaggaacg
     8641 gcagatcgat ctgcggcgac accggcacga tttcgagcgg cggcaccgcc tggcgccgtg
     8701 cgaaatagcc ggcgagcggc ccccacacca ccgccacgtc gatctccccc gccgccaccg
     8761 cctcgacgat ccgcgccgga gggttcggct cccggtagtc gccatacacc gaatatcccc
     8821 ggacgttgtc gatcacaccg cggctcgcca gcgcgtgagc gggaggagca ttgtgtaaat
     8881 cgtcgccgat catctgcacc ccgacccgca gccgccgcag cgccggatca tcgaacgacc
     8941 gcaccgtgat cccgctgtcc ttcggataga cgaacacata gctcgaccgg tagtaaggac
     9001 gggtagccat cgccagctcg aagctgctgg ggacgcccat caccacgtcg cagagtcccg
     9061 cccgcagcgt gttcctgaag aacccccgcc gctgcgccca ccacgtgtac tccacccgcg
     9121 cccccatctc gcgagcgatc accttcgcga tctcgttctc gaatccttcg agctgccggt
     9181 tcgaaaacgg cagattgttg ggatcagcgc agacgcggag cgtgcgggcg gggggggcgg
     9241 gggcgcagga gatgaagaga gctgcgatca agaagaagag gagcaccggg gcggcctgcc
     9301 cgcgagtcct caccccccgg ccccctctcc catctccctt ccccccaatc ccgggagagg
     9361 gggagaaaaa cacagccttc tcttgggctg ttctccccct ctcccggcgg ggggcagggt
     9421 gggagatggg agagggggcc ggggggtgag aactccgcgc gtacgaatcc cccgcctcca
     9481 cgttcgtccc cttacagcga gaacacataa agcatccccc cgcgagtcgt cgcctccttc
     9541 agatccgcca tcgcattggc aaaccccagc gccgccgtgc cgtcccgcgg atcgagatcc
     9601 cccgcgacca cggcccccgc ccagcccccc acgcccgaga ggatggcaac gtattgcttg
     9661 ccgtccggtc cgcgataggt cacgggctgg ccgatgatcc cggagccggt cttgaacttc
     9721 cacaactcct gtccattgcg ggcgtccagc gccttgaacc agccatccat ggtgccgtag
     9781 aagaccacgt cgcccgcggt gaccagtgcg ccgctccagg ccgggaactt ctccttgatc
     9841 tgccaacggg gcttgccggc caccgggtcc caggccgtga agaacccccg gttgccaccg
     9901 gggccggcgt acatcttcac gttggcgccg acgaacggcg tgcctgcgat gtagttcgcc
     9961 tcgaccccct cgtaatccat gcagagattg ttgtggggca tgtaaaggag acccgtgcgc
    10021 ggcgagaaag cggacggctg ccagtccttg gcgcctggcg acgcggggca gatctcgcgc
    10081 accaccttgc ccacaccggt cttcttggcc tccacctcct ggatccggcc ggttttcaga
    10141 tcgacgccgg tcgagacggt gatcggagcg aatgggttgg ccgacagcac ctgaccggtg
    10201 gcgcgatcca ttacatagac gtagccgttg cgctccggcc gcacgagcac cttgcgtttg
    10261 cggccaccaa cctcgagatc cagcagaatg ctctcgttga tgccgtcgta atcgaaatga
    10321 tcatgagggc cgacgtgata gaaccagcgg gcctgtccgg tgtcgggatc gcgggcgaag
    10381 acgccgcagg tccacttgtt gtcgccgggc cgctggtccg ggttccacgg gccgggattg
    10441 ccggtgccat agaagatgag gtcgagctcg ggatcgtagg agatccaccc ccaaaccgtg
    10501 ccgccgccca tcttccaggc gtcggggggc cagctcttga cgccgaggtc cgtgccctgg
    10561 tccatgggat agaacggctt gaactcgggg ccgatcagga cgtctttgtc cgggccggtg
    10621 ctgtaggcgc gccaagcgat cttgccgctg ccggcgtcga gggcggtcag ccatccgcgc
    10681 acgcccagct cgccgccgct gttgcccacg agcaccttgt ccttgaccac caggggcgcc
    10741 atggtcatgg tctcgccctt gttgatgtcc cccagcttgg tcttccagac ctctttcccg
    10801 gtctccgcgt ccacggcgac cgtgtggttg tcgagggtgt tgtaaaagat tcgcccgttg
    10861 gcataggcgg caccccgatt gaccacgtcg cagcacgcga ccccctgggc ggcgggatcg
    10921 ggctgcggct catagaccca cttcgccgga gcgcccggct gggtgagatc gagcgcgtag
    10981 agctggttgg ggtacggggt gacgacgtac atcgtgccgc cgaccacgag cggcgcggcc
    11041 tcgtggcccc gcaggctgcc ggtggagaac gtccacgcca ctttgagatt cttggcgctc
    11101 tggacgttga tctggccaag tccgctgaac cgggtgctgg cgtagttctt ggccgccatc
    11161 acccactggc cgtcgtcggc ctggaggtct gccggcgaca gcttccccga gatccgcccc
    11221 gccccctggc ccacgtgggg ccccgaggcg cgctcttccc caccgcatcc gaaggccaag
    11281 acactgagac tgcaaaggaa agcgaccgtg aaaccgacac cccacccgcc cggaccggtt
    11341 ctctccatca gcgttcaaac actcctctcg agaatcctga acagccctcg ccggcatcca
    11401 ggcccgcacc tttcccctcg gcgctctgag ctcccagacc gttcctgtct tccccacaca
    11461 gaacaaaggc tcacggtgga taaaagcaca gcaaagtaga tgccaaggag gagcttcggg
    11521 gattcccaac ggggtcgtgc cagccgcccc gtcatcctga gaggagccct ggcg
//
LOCUS       DASPJI010000772         4621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_449846, whole genome shotgun sequence.
ACCESSION   DASPJI010000772 DASPJI010000000
VERSION     DASPJI010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449846"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VEL74_21610"
     CDS             <1..487
                     /locus_tag="VEL74_21610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65193.1"
                     /translation="PAPPAEGGPREVFGVSATAALFRRSALPAGDVFDPRLGSYYEDV
                     DLAVRLRADGWRALLVPAARARHAGSATGKTLGSRRWRLIHGNRHLVAARLLGRSYWT
                     RLPRLVLRDLVDLGRAALTGEGALAAGIAGGWGRAARLLPGFARRGAPAVPLRDVARF
                     R"
     gene            498..1436
                     /locus_tag="VEL74_21615"
     CDS             498..1436
                     /locus_tag="VEL74_21615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG65194.1"
                     /translation="MEGRDPAAPLLSGVVVHWHNEELLAGLAEAWPRDPRFELVVVDN
                     GSQSELAAGPARVLHPGRNLGFAGGANAGAEAARAPILLILNPDAVPEPGALDRLLEG
                     FAACPDAAGLAPCLSGPGGEPQWAWQLRPLPTPWSLLRHAFPLPGGEGVSREPAAGEP
                     VEQPAAAALALRREALAAVGGFDTGFHPAWFEDVDLARRLRDAGLVLRYWPAARFRHG
                     LGSTVPRLGYGPFLWIYHRNLDRYLAKHHGRGWALASRLLLAPGLLLRLALLPLRRPR
                     RATSRGDAARGLALTLAGALTGWRRPRALAFGSARA"
     gene            1433..2485
                     /locus_tag="VEL74_21620"
     CDS             1433..2485
                     /locus_tag="VEL74_21620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG65195.1"
                     /translation="MTGVPERPAVAVAVAVAVCIVTHNDAADLPGCFAALAGVTHRPL
                     EVIVADCASRDGSPEVARREAAARIPDIPCQVVELGENRGFAGGMNAAFARTGAPFVM
                     TLNADAHLGPDYITRLLERARAHPGLRVGGLTGRILRFPRDAEGNEGEPRLLDACGMY
                     LTANWRHLDRGSGETDHGQWGRPERVFGATGAAALFSREALLDVAVDGEIFDSRFHTY
                     REDAELCFRLRERGWEILYEPAALAEHRRFNLPQRRSAMPEAANYNSLKNRYLLRLYH
                     QTGANFLRTFFPTLGRDLVALAYVLLKEHSSLPAYSWLWRHRREILRWRRIIQGRRTV
                     PAGEVDRWFHVQGEPL"
     gene            2482..3630
                     /locus_tag="VEL74_21625"
     CDS             2482..3630
                     /locus_tag="VEL74_21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HYG65196.1"
                     /translation="MTRPLSVALIGSRGIPGRYGGYETLLEELATRLVERGFRVTVYC
                     RSHSTPRSLTSFRGVDLVVLPTVRTKYLDTPVHTLLSCFHAMGGGYDAALVVNSANAL
                     FVPLLRLGGVPVALHVDGIEKRRAKWGPVGRGVYALSERLACVLPDELVTDAEVIRRH
                     YLDRYGAESVPIAYGVDPRPIPDGGDTGVLARLGLESRRFFLYVSRFEPENNPHRVAE
                     AYRSVGGDLPLVMVGGAPYAGEFIAGFTRGADPRVLFPGAIYGEGYRELLSHALAYIH
                     ATEVGGTHPALVEAMGYRNCSVVNDTPENREVTGDAGLYFRAAEPVTLAERLDWVRQH
                     PDEARRLGIRAGERAAGLYSWERVTGLYARLFERLAGHLAGRRGSPSG"
     gene            3763..>4621
                     /locus_tag="VEL74_21630"
     CDS             3763..>4621
                     /locus_tag="VEL74_21630"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65197.1"
                     /translation="MTAAFLLAFAVRSWLLPAIAPGAFPSHLYPLPEYLPLLPLALAI
                     WGVLLLSSGRYRSHRTVPLVDEALAVIRVCISGSVIFTLVLYAARLDGRLLGNDQISR
                     SWILLFGIFSVLLLLTEKLGVRLTSRYVRMHGFNYRTVLIVGTGPTALRIADSIQSHR
                     FWGFRILGFVVNGDAPGQPWGSRYPVLGEIADIPRIVESNVVDDVIFAVHRRDLDRLE
                     DLFLSLQEQGIRTRFAMDLFPHARARVELEELDGMPLLSFATTPTSQLQLMAKRLLDV
                     AIAALLLVLA"
BASE COUNT          526 a         1799 c         1604 g          692 t
ORIGIN      
        1 gccggcgcct cccgccgagg ggggaccgcg cgaggtcttc ggcgtctcgg cgaccgccgc
       61 gctcttccgg cggagcgccc tgccggccgg cgacgtcttc gacccccggc tcggctccta
      121 ctatgaggat gtcgacctcg ccgtccgcct gcgggcggac ggctggcggg ccctcctggt
      181 gccggcggcg cgggcgcggc acgccggctc ggcgacgggg aagaccctgg ggagccgccg
      241 ctggcgcctg atccatggca accgccacct ggtggcggcc cggctcctgg gccggagcta
      301 ctggacccgc ctgccgcgcc tcgtcctgcg cgacctggtg gacctgggcc gcgccgcgct
      361 gaccggcgag ggggccctgg cggccggcat cgccggcggc tggggacggg cggcccgcct
      421 cctccccggc ttcgcccgcc gcggcgcccc cgccgtgccg ctacgggatg tggcgcgctt
      481 ccggtaacct tcgcgccatg gaagggagag accccgccgc cccgctcctc tccggagtgg
      541 tggtgcactg gcacaacgag gagctgctcg ccgggctcgc cgaggcctgg ccgcgcgacc
      601 cccgtttcga gctggtggtg gtggacaacg gttcccagtc agagctggcg gccgggccgg
      661 cccgggtcct ccatccgggc cgcaacctgg gcttcgccgg cggcgccaac gccggggccg
      721 aggcggcccg tgccccgatc ctcctgatcc tcaacccgga cgcggtgccc gagcccggcg
      781 ccctcgaccg tctcctggag gggttcgccg cctgcccgga cgccgcgggc ctcgctccct
      841 gcctctccgg gcccggcggc gagccgcagt gggcctggca gctccggccg ctccccaccc
      901 cctggagcct gctgcgccac gcgttcccgc tgccgggcgg cgagggggtc tcccgcgagc
      961 ccgccgccgg tgagccggtg gagcagccgg ccgcggcggc gctggccctg cggcgcgagg
     1021 cgctggcggc ggtgggcggc ttcgacaccg gcttccatcc cgcctggttc gaggacgtgg
     1081 acctggcgcg ccggctgcgc gacgccgggc tggtgctgcg ctactggccg gcggcccgct
     1141 tccgtcacgg gctggggagc acggtgccgc ggctcggcta cggccccttc ctctggatct
     1201 atcaccgcaa cctggaccgc tacctggcca agcaccacgg ccgcggctgg gcgctggcgt
     1261 cgcggctcct gctggcgccc ggactgctgc tgcggctggc gctcctgccg ctacgccggc
     1321 cacggcgggc cacctcgcgc ggcgacgccg cccgcgggct cgccctcacg ctggccggcg
     1381 ccctcaccgg ctggcgccgc ccccgcgccc tggccttcgg gagcgcccgg gcgtgaccgg
     1441 cgtccccgaa cggccggcgg tggcggtggc cgtggccgtg gccgtctgca tcgtcaccca
     1501 caacgacgcg gcggacctgc ccggctgctt cgcggccctc gccggcgtca cccaccggcc
     1561 gctggaggtg atcgtcgccg actgcgccag ccgcgacggc agcccggaag tggcccgccg
     1621 cgaggccgcg gcccgcatcc cggacatccc ctgccaggtg gtggagctgg gggagaaccg
     1681 cggcttcgcc ggggggatga acgcggcctt cgcccgcacc ggggcgccgt tcgtcatgac
     1741 cctgaacgcc gatgcgcacc tcgggcccga ctacatcacg cggctcctgg agcgggcgcg
     1801 ggcccacccc ggtctgcggg tggggggcct caccggccgc atcctgcgct tcccccgcga
     1861 cgccgagggg aatgaagggg agccgcgcct cctggacgcc tgcggcatgt acctgaccgc
     1921 caactggcgg cacctcgacc gcggctcggg agagaccgac cacggccagt gggggagacc
     1981 cgagcgggtc ttcggcgcca ccggtgccgc cgccctgttc agccgcgagg cgctcctcga
     2041 tgtcgcggtc gacggcgaga tcttcgactc ccggttccac acctaccggg aggacgccga
     2101 gctctgcttc cggctgcggg agcggggctg ggagatcctc tacgagcccg ccgccctggc
     2161 tgagcaccgc cgcttcaacc tgccgcagcg ccgctccgcc atgccggagg ccgccaacta
     2221 caactcgctc aagaaccgct acctgctgcg gctctatcac cagaccgggg ccaacttcct
     2281 gcgcaccttc ttcccgaccc tgggccgcga cctcgtggcg ctcgcctacg tcctgctcaa
     2341 ggagcactcg tccctgcccg cctactcctg gctctggcgc caccgccggg agatcctccg
     2401 ctggcggcgg atcatccagg ggcggcgcac cgtgccggcg ggcgaagtcg accgctggtt
     2461 ccacgtccag ggggagcctc tttgaccaga cctctttccg tcgccctgat cggcagccgg
     2521 ggcattcccg gccgctacgg cggctacgag acgctgctgg aggagctggc cacgcggctg
     2581 gtggagcgcg gcttccgggt gaccgtctac tgccggtccc actcgacccc ccgctcgctc
     2641 acctcgttcc gcggcgtgga cctggtggtg ctgccgaccg tgcgcaccaa gtacctggac
     2701 acgccggtcc acaccctcct ctcctgtttc catgccatgg gcggcggcta cgacgcggcg
     2761 ctggtggtca actcggccaa cgcgctgttc gtcccgctgc tgcggctggg cggcgtgcct
     2821 gtcgcgctgc acgtcgacgg catcgagaag cgccgtgcca agtgggggcc ggtgggccgg
     2881 ggcgtctatg ccctctccga gcggctggcc tgcgtgctgc ccgacgagct ggtgaccgac
     2941 gccgaggtca tccgccgcca ctacctggac cgctacgggg cggagtcggt gcccatcgcc
     3001 tacggcgtcg atccccggcc gattccggac gggggcgaca cgggggtcct ggcgcggctg
     3061 gggctcgaga gccgccgctt cttcctctat gtcagccgct tcgagcccga gaacaacccc
     3121 caccgggtgg ccgaggccta ccgctcggtg ggcggcgacc tgccgctcgt gatggtcggg
     3181 ggggcgccct acgccggcga gttcatcgcc ggcttcaccc gcggcgccga tccgcgcgtc
     3241 ctcttccccg gcgccatcta cggcgagggg taccgggagc tcctctccca cgccctggcc
     3301 tacatccacg ccaccgaggt ggggggcacc caccccgccc tggtggaggc gatgggctac
     3361 cgcaactgct ccgtagtcaa cgacacgccg gagaaccggg aggtgaccgg cgacgccggc
     3421 ctctacttcc gggcggcgga gccggtgacc ctggcggagc ggctggactg ggtgcggcag
     3481 cacccagacg aggcgcggcg gctcggaatc cgcgccggtg agcgggccgc cggtctctac
     3541 tcctgggaac gagtcaccgg cctgtatgcc cgccttttcg aacgtctggc cggccatctg
     3601 gccgggcgcc ggggctctcc gtctggatga aatgcccggc acatacgtac aatagacagt
     3661 gggctacgtg aagctcgcgc caaaagacac ctcggaccat caatgctgaa agaacgagcc
     3721 cggattctca cggtcggaat ttttctgctg gatctggccc tggtgacggc ggccttcctg
     3781 ctggccttcg ccgtccgcag ctggctgctg ccggcgatcg cgcccggggc cttccccagc
     3841 catctctacc ccctgccgga gtatctgccg ctgctgccgc tggccctggc catatggggc
     3901 gtcctcctcc tctcctccgg ccggtaccgt tctcaccgca cggtgccgct ggtggacgag
     3961 gcactggccg tcatccgcgt ctgcatcagc ggctcggtga tcttcaccct ggtgctctat
     4021 gccgcccggc tggacggccg gctcctggga aacgaccaga tcagccgctc ctggatcctg
     4081 ctcttcggca tcttctcggt cctgctgctc ctcaccgaga agctgggcgt ccgcctgacc
     4141 tcccgctacg tgcggatgca cgggttcaac taccggaccg tgctcatcgt cggcaccggc
     4201 ccgacggcgt tgcgcatcgc cgactcgatc cagagccacc gcttctgggg cttccgcatc
     4261 ctcggcttcg tcgtcaacgg cgacgccccc ggccagccgt gggggagccg ctatcccgtc
     4321 ctgggcgaga tcgccgacat cccgcggatc gtcgagagca acgtggtgga cgacgtcatc
     4381 ttcgccgtcc accgccgcga cctcgaccgg ctggaggacc tcttcctgag cctccaggag
     4441 caggggatcc gcacccggtt cgccatggac ctcttccccc acgcccgggc gcgcgtcgag
     4501 ctggaggagc tcgacggcat gccgctgctg tcgttcgcca ccacccccac ctcgcagctc
     4561 cagctcatgg ccaagcgcct gctggacgtg gcgatcgccg ccctgctgct ggtgctggcg
     4621 c
//
LOCUS       DASPJI010000773         2353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_618130, whole genome shotgun sequence.
ACCESSION   DASPJI010000773 DASPJI010000000
VERSION     DASPJI010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618130"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VEL74_21635"
     CDS             <1..880
                     /locus_tag="VEL74_21635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HYG65198.1"
                     /translation="VVVETRGRIRDSFETYRAEGRFPQIDVPVVVLVNEGSASASEIV
                     AGAVQDHDRGLVVGQPSWGKGLVQTVYNLSYGAAIALTTAKYYTPSGRLIQRDYTSYF
                     DYYTHADAGTPEMLGKTVREDEIFRTDLGRKVYGGGGITPDVIVENDPLNEFAQFLLA
                     RNAYFTFAVDYNRRNPVKSTDWKPAPQVLDEFKKWVISEGISPAAEVEKEFADAKLQE
                     QALTQIRAEVLNAVSGQEARHRAFAQIDKQIQTALAQFDKAAELYAQRKLLDQKDEER
                     ADTAAAAEATSRAPRG"
     gene            complement(956..>2353)
                     /locus_tag="VEL74_21640"
     CDS             complement(956..>2353)
                     /locus_tag="VEL74_21640"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HYG65199.1"
                     /translation="EFGVEGTIEGISPGPVITVFELQPAPGVKVSQVVNLQDDLALAL
                     RAESVRIDRMAGRSTLGIEVPNRERGVIRLGTLLADDRFKKAPSVLTMAIGTDIHGTP
                     YYADLATMPHLLVAGATGAGKSVGLQSMITSILYKATRDDVQFIFIDPKRIELGVYAD
                     IPHLKAEVVVDPKKAANALRWAVAEMERRYRLLAEVHVRSIAYYNRAIQDPEVQQRLA
                     LNEGEAPVTPADLKPLPYYVIIIDELADLMMVASSDVETSIARLAQMARAVGIHLIVA
                     TQRPSVDVLTGTIKANFPCRIAFATASRHDSRTILDQVGSEKLLGKGDMLMMPQGSSR
                     VIRLHGAYTSEQETAALVRWLKKQGKPELDPDVLREPAGDSGSGTDGGDNDDELYDEA
                     ARLVVAERQASASFLQRRMRIGFSRAARLIDLMARDGLLGPSQGSKPREVLVPPDYFE
                     EVSDARSAVEGDYEE"
BASE COUNT          365 a          829 c          809 g          350 t
ORIGIN      
        1 cgtcgtcgtc gagacccgcg gccgcatccg cgactccttc gagacctacc gggccgaggg
       61 gcggttcccg cagatcgacg tgccggtggt ggtgctcgtc aacgagggca gcgcctccgc
      121 ctccgagatc gtggccggcg ccgtccagga ccacgaccgc ggcctggtgg tcggccagcc
      181 gagctggggc aaggggctgg tccagaccgt ctataacctc tcctatggag cggccatcgc
      241 gctgaccacg gccaagtact ataccccgtc ggggcggctg atccagcgcg actacacctc
      301 ctacttcgac tactacaccc acgccgacgc cggcaccccc gagatgctcg gcaagacggt
      361 gcgcgaggac gagatcttcc gcaccgacct cggacgcaag gtctacggcg gcggcggcat
      421 cacccccgac gtcatcgtcg agaacgatcc gctgaacgag ttcgcccagt tcctcctggc
      481 gcgcaacgcc tacttcacct tcgcggtcga ctacaaccgg cgcaacccgg tcaagtcgac
      541 ggactggaag ccggcgccgc aggtgctgga cgagttcaag aagtgggtga tcagcgaggg
      601 catcagcccg gccgccgagg tcgagaagga gttcgccgac gccaagctgc aggagcaggc
      661 gctgacgcag atccgcgccg aggtcctgaa cgccgtgtcc ggccaggagg cccgtcaccg
      721 cgccttcgcc cagatcgaca agcagatcca gaccgccctc gcccagttcg acaaggccgc
      781 agaactctac gcccagcgca agctcctcga tcagaaggac gaggagcggg ccgacaccgc
      841 ggccgccgcc gaagccacga gcagggcccc ccgcggctga gcttcacccc ttccgccaga
      901 aacacgaacg gccggcgcga gccggccgtt ctgcttcagc ccctcaacct accccctact
      961 cctcgtagtc cccctcgacc gcgctgcggg cgtccgacac ctcctcgaag tagtcgggcg
     1021 ggaccaggac ctcgcgcggc ttgctcccct gggaggggcc gaggaggccg tcgcgcgcca
     1081 tcaggtcgat gaggcgggcg gcgcgggaga agccgatgcg catgcggcgc tggaggaagc
     1141 tggcggaggc ctggcgctcg gccaccacca gccgcgccgc ctcgtcgtac agctcgtcgt
     1201 cgttgtcgcc gccgtcggtg ccggagccgg agtccccggc gggctcgcgg agcacgtccg
     1261 gatcgagctc gggcttgccc tgcttcttga gccagcgcac cagcgccgcc gtctcctgct
     1321 ccgaggtgta ggcgccgtgc aggcggatca cccgcgagct gccctgcggc atcatcagca
     1381 tgtcgccctt gcccaggagc ttttccgagc ccacctggtc gaggatcgtc cgcgagtcgt
     1441 ggcggctcgc ggtggcgaag gcgatgcggc aggggaagtt ggccttgatg gtgccggtca
     1501 ggacgtctac cgacgggcgc tgcgtcgcga cgatcaggtg gatacccacg gcgcgcgcca
     1561 tctgggcgag ccgggcgatg gaggtctcga cgtccgagga ggcgaccatc atgagatccg
     1621 ccagctcgtc gatgatgatc acgtagtagg gcagcggctt caggtccgcc ggggtgaccg
     1681 gggcctcgcc ttcgttgagc gccagccgct gctgcacctc gggatcctgg atcgcccggt
     1741 tgtagtaggc gatcgagcgc acgtgcacct cggcgaggag acgatagcgg cgctccatct
     1801 cggccaccgc ccagcgcagc gcgttcgccg ccttcttcgg gtccaccacc acctcggcct
     1861 tgaggtgcgg gatgtcggcg tagaccccca gctcgatccg cttcgggtcg atgaagatga
     1921 actgcacgtc gtcgcgcgtc gccttgtaga ggatcgaggt gatcatgctc tgcaggccca
     1981 cgctcttgcc cgcgccggtg gcgccggcga ccagcaggtg cggcatggtg gcgaggtcgg
     2041 cgtagtaggg ggtgccgtgg atgtcggtgc cgatcgccat ggtgagcacc gacggcgcct
     2101 tcttgaaccg gtcgtcggcg agcagcgtcc ccaggcggat gacgccgcgc tccctgttcg
     2161 gcacctcgat gcccagggtg gagcggccgg ccatgcggtc gatgcgcacc gactcggcgc
     2221 ggagcgccag cgccaggtcg tcctggaggt tgacgacctg gctgaccttg acgccggggg
     2281 cgggctggag ctcgaagacg gtgatcaccg ggccggggct gatcccctcg atcgtcccct
     2341 cgacgccgaa ctc
//
LOCUS       DASPJI010000774         5845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_449999, whole genome shotgun sequence.
ACCESSION   DASPJI010000774 DASPJI010000000
VERSION     DASPJI010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449999"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VEL74_21645"
     CDS             complement(<1..251)
                     /locus_tag="VEL74_21645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65200.1"
                     /translation="MNSFSQGSTVSTQSPLPNAFTPAFLGLLAERDEPPTAGDADVAG
                     PWRIEPIPDHGFGLFRVGESLARGFAPTAVFPSRWLALL"
     gene            535..741
                     /locus_tag="VEL74_21650"
     CDS             535..741
                     /locus_tag="VEL74_21650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65201.1"
                     /translation="MTLKGFEVVDEEMARVLREKTGAERLQIAWRMFASARAMLESHL
                     RSEHPDWSEQQIRAEAVRRLSLGG"
     gene            761..1309
                     /locus_tag="VEL74_21655"
     CDS             761..1309
                     /locus_tag="VEL74_21655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65202.1"
                     /translation="MEVLERLGLRYLVTGSVATIFFGEPRFTVDIDIVVDLPAERIRD
                     FCAAFPSGEFYLSEETVRRAVRNRSQFNIIHPSSGLKVDVMIPSGTPFDHSRFARMRR
                     IRPGQDFEASFASPEDVIVKKLEYYREGGSQKHLRDIGGVLRISGEKIDFSYIQEWAE
                     RLGLDEIWQAILDELDHPSRNS"
     gene            1542..2108
                     /locus_tag="VEL74_21660"
     CDS             1542..2108
                     /locus_tag="VEL74_21660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65203.1"
                     /translation="MKKLYVGTVLTLALLFAPQMASAQTPCTAVCQAHYPCDYSCDLC
                     VGDPGLWEDGSGCWGEIVSGTCGDIGQCGWVQPPTCTPNWIYDEISHQGAIPELTWDP
                     VCEWQWNHPSGPQYVCDYDKPFKCTVYGVNKYVRHQDNCSPSSSQTFCGYAWQSYVGH
                     YSNSWSTWECCLDSYEWNCTTSFPQCGW"
     gene            complement(2237..4036)
                     /locus_tag="VEL74_21665"
     CDS             complement(2237..4036)
                     /locus_tag="VEL74_21665"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYG65204.1"
                     /translation="MVNDDTPTRSFGDLGLSEPLLKALTDAGYEAPTPIQEKTIPALL
                     SGRDLIGQAQTGTGKTAAFSLPILQRLDLTGGGAGVQALVLVPTRELALQVAEALHIY
                     SRYLGRVSVLPIYGGQPIQVQLQRLQRGVHVVVGTPGRVMDHLRRGTLDLGTVRTVVL
                     DEADEMLRMGFIDDVEWILSQATSTGAGRQTALFSATLPAEVRRIANRHLQDPAETQI
                     EHRTLTVPAIEQRYLNVSHQQKLEALTRLLETEPGGAVLVFVRTKTGAAELTEKLQAR
                     GYAAESLHGDMGQVQRESVVRRLRSGQVEIVVATDVAARGLDVERIGHVINYDIPNDV
                     EAYVHRIGRTGRAGRTGVAVLFVTPRERRMMQEIERFTGQRLSAMRVPTQADVAARRI
                     ALFKESIKKTIAEGDLDLYLTLVEELAQEEGLDMAEVAAAAARLARGDKPLEVELEPE
                     PTAMPAAEDGMVRLFLAAGSRAGVRPSDIVGAIANEAGIPGKSIGSIDIYDRFTFVEL
                     PLQYKDQVMERMANATIRGRPVDIRIARPGTPAPERREGGEERGQRPWTARPRGPGGP
                     GGGRPGGRPGGPPTGRRSGPGGGPGKPPRRGQG"
     gene            4095..4439
                     /locus_tag="VEL74_21670"
     CDS             4095..4439
                     /locus_tag="VEL74_21670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65205.1"
                     /translation="MPERMLYQKLNAEMRTMVDRYAERLRVYDWGRRSELLSGTALPF
                     GEDLPPEQARKAAVGFVTAVLEKLGEDEVTDFRQARLYLLSLNPGHRELAEQYLEANP
                     EAREAIEREMGD"
     gene            4447..5271
                     /locus_tag="VEL74_21675"
     CDS             4447..5271
                     /locus_tag="VEL74_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HYG65206.1"
                     /translation="MSRPWETLDAAETREGLLELRRRGDEFVILIGGRVLMNSALHLT
                     EDAVAERACREVAGRPRPRVLRVLIGGLGMGYTLRAALDALPGSARVLVAEIDPVIVR
                     WCQGPLAHLTGRALEDKRVQVEVADVGAVIATAAKEGAADPRRLFDAIVLDLYEGPRE
                     AHGGQGDPLYGRRALERARAALAPGGVLSVWSEDADAAFAERLRTAGFEVQRLRPGKG
                     GPRHVVYLARASSSAAASRATPSRMAGGSTGAKPRSKASRRGASRPSRPPSRSPRQ"
     gene            complement(5340..5795)
                     /locus_tag="VEL74_21680"
                     /pseudo
     CDS             complement(5340..5795)
                     /locus_tag="VEL74_21680"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
BASE COUNT          876 a         1947 c         2117 g          905 t
ORIGIN      
        1 atgaggagcg ccagccagcg ggaagggaag accgccgtcg gggcgaagcc gcgggccagg
       61 ctctcgccca ctcggaacag gccgaaccca tgatccggaa tgggctcgat gcgccaaggc
      121 ccggccacgt cggcgtcccc ggccgtgggc ggctcgtccc gctccgcgag caggccgagg
      181 aaggctgggg tgaaggcgtt gggaaggggg gattgagtgg agacagtgct accctgcgag
      241 aagctgttca taagtcctcc tacggactgt gagcggtcag gctcggaggt gaggaccagt
      301 cacctccggg ccgtcagcag gattgccgac cgccctttgc agcgcttcta catctggatg
      361 ccacttggca cccctggggc gaccgaggtc gtctcatggg gtgcaaggga agcatatggt
      421 aggcgtgggg gtggttgcaa gggtgtgggg aagatttctt gacccctctc ccaagttgcg
      481 caccggggcc tccttgccgg tggtgcagta cttggctatc gtggctccat gggaatgacg
      541 ctcaagggtt tcgaggtcgt cgacgaggag atggctcgcg tgctgcggga gaagaccggg
      601 gccgagcggc tgcagatcgc ttggcgcatg ttcgcctcgg caagagcgat gctcgagagc
      661 catctccggt ccgagcaccc ggattggagt gagcagcaga tccgcgctga ggcggtccgg
      721 aggctctcgc ttggaggata aggatctgct ccggtacgcc gtggaggtcc tggagcggct
      781 tgggctccgc tacctcgtca ccggctccgt ggccactatc ttctttggcg agccccggtt
      841 taccgtcgat atcgacatcg tggtcgatct gcccgccgag cgcattcgcg atttctgcgc
      901 cgccttccct tctggagagt tctacctcag cgaggagacc gtcaggcgcg cggtccgaaa
      961 ccggagccag ttcaacatca ttcatccctc gtccggcctc aaggtcgacg tcatgatccc
     1021 ctccggcaca ccgttcgacc acagccggtt tgccaggatg cggcgcatca ggccggggca
     1081 ggatttcgag gcctcgttcg cctcccccga ggatgtcatc gtcaagaagc tcgagtatta
     1141 ccgggaaggg ggctcgcaga agcacctccg ggacatcgga ggcgtgctga ggatcagcgg
     1201 cgagaaaatc gacttttcct atatccagga atgggccgaa cgccttggcc tggacgagat
     1261 ctggcaggcg atcctggacg agctggacca tccctctcga aacagctgaa agcacctacc
     1321 atcaccccag acctgacacc gcgacggcag aggtcccatc gctggggcga ctccttcgag
     1381 cggggctcgc gcacccaaga aggtggaggc tcagatgtcc gaggcgcgag cccttccccc
     1441 ctccccgctg cccgggggag ggtagagaag gtcctttgcg catcggctcg accgcagtag
     1501 ctttgagctg cctaatggga ggcagcgaaa aggagggtgc catgaagaag ttgtacgtag
     1561 gtacagttct gacgttggct ctgctgttcg ctcctcagat ggcttccgcg cagaccccat
     1621 gtactgcggt ctgccaggcg cactacccct gcgactatag ctgtgacctc tgtgtcggcg
     1681 acccgggatt gtgggaggac ggcagcggct gctggggcga gatcgtctcc gggacctgcg
     1741 gcgatatcgg gcaatgtggc tgggtgcagc cgccaacctg tacccccaat tggatttatg
     1801 acgagatttc acatcaaggg gccataccgg aactcacctg ggaccccgtc tgtgaatggc
     1861 agtggaatca tccctcaggg ccacagtacg tctgcgacta cgacaagcca ttcaagtgca
     1921 cggtctacgg cgtcaataaa tatgttcggc accaggacaa ctgctctcca tcgtcgtcgc
     1981 agaccttctg tggctatgcc tggcaaagct acgtcggcca ctactcaaat tcctggtcaa
     2041 cctgggagtg ttgcctcgac tcctacgagt ggaattgcac cacgtcgttc cctcagtgtg
     2101 gttggtagcc ccgaggcgag gctgaccgcc cttaaaggca gtggcaggcg caggacctca
     2161 gcatctggag atcctgggcg ggccacgagc gaccggcgcg tcgtagtccc ttctacgacg
     2221 aacgaaaggg agacacctac ccctgaccgc gccggggggg cttgcccggt ccgccgccgg
     2281 gtccgctccg ccggccggtc ggggggccgc cgggccttcc tcccggccgg ccgccgccgg
     2341 ggccaccggg accgcgaggc cgggcggtcc atggccgctg cccgcgctcc tctcccccct
     2401 cccgccgctc gggggccggg gtgcccgggc gggcgatgcg gatgtccacc ggccggccgc
     2461 ggatggtggc gttggccatc cgctccatca cctgatcctt gtactgcagg ggcagctcga
     2521 cgaaggtgaa gcggtcgtag atgtcgatcg agccgatgga cttgccgggg atgcccgctt
     2581 cgttggcgat ggcgccgacg atgtccgacg gacggacgcc ggcgcggctg ccggcggcga
     2641 ggaagagccg caccatgccg tcctcggcgg cgggcatcgc cgtcggctcg ggctccagct
     2701 ccacctccag cggcttgtcg ccgcgcgcca ggcgggcggc ggcggcggcg acctcggcca
     2761 tgtcgaggcc ctcctcctgc gccagctcct cgaccagcgt caggtagagg tcgaggtccc
     2821 cctcggcgat cgtcttcttg atgctctcct tgaacagggc gatgcgccgg gcggcgacgt
     2881 ccgcctgggt gggcacccgc atggcggaga ggcgctggcc ggtgaagcgc tcgatctcct
     2941 gcatcatccg ccgctcgcgc ggggtgacga agaggacggc gacgccggtg cggccggccc
     3001 ggccggtgcg gccgatgcgg tggacgtagg cctccacgtc gttcgggatg tcgtagttga
     3061 tgacgtgacc gatacgctcg acgtcgaggc cgcgggccgc cacgtcggtc gccacgacga
     3121 tctccacctg gccgctgcgc aggcgccgga cgacgctctc gcgctgcacc tggcccatgt
     3181 cgccgtgcag ggactcggcg gcgtacccgc gggcctggag cttctcggtc agctcggcgg
     3241 cgcccgtctt ggtgcggacg aacaccagga ccgcgccacc gggctcggtc tcgagcagcc
     3301 gggtgagcgc ctcgagcttc tgctggtgcg agacgttcag gtagcgctgc tcgatggccg
     3361 ggacggtgag ggtccggtgc tcgatctggg tctccgccgg gtcctggagg tggcggttgg
     3421 cgatgcgccg cacctcggcc ggcagggtcg ccgagaagag cgcggtctgc cgcccggcgc
     3481 cggtcgacgt ggcctgggag aggatccact cgacgtcgtc gatgaacccc atgcgcagca
     3541 tctcgtcggc ctcgtcgagc acgacggtgc gcacggtgcc gaggtcgagg gtgccgcgcc
     3601 gcaggtggtc catgacccgc cccggcgtgc ccaccaccac gtggacgccg cgctgcagcc
     3661 gctggagctg cacctggatc ggctggccgc catagatcgg cagcacgctg acccggccca
     3721 gatagcggga gtagatgtgg agcgcctcgg ccacctgcag ggccagctcg cgcgtgggga
     3781 cgaggaccag cgcctgcact ccggcccctc cccctgtgag gtcgagccgc tggaggatgg
     3841 ggagggagaa ggccgcggtc ttgcccgtgc cggtctgcgc ctggccgatc aggtcgcggc
     3901 cggagaggag cgccgggatg gtcttctcct ggatgggggt gggggcttcg tagccggcgt
     3961 cggtcagggc cttgaggaga ggttccgaaa ggccgagatc gccgaaggag cgggtggggg
     4021 tgtcgtcgtt caccatccta ttgtacagat tgcacccttc gtgccgtgta tcctggggaa
     4081 gggaaggagg gccgatgccc gagaggatgc tctaccagaa gctgaacgcc gagatgagga
     4141 cgatggtgga ccgctacgcc gaacggctcc gggtctacga ctgggggcgg cgcagcgagc
     4201 tcctgtccgg gaccgccctg ccgttcggcg aggacctgcc gccggagcag gcccggaagg
     4261 cggcggtggg gttcgtcacc gccgtcctcg agaagctggg cgaggacgag gtcaccgatt
     4321 tccggcaggc ccgcctctat ctcctctcct tgaaccccgg gcaccgcgag ctggccgagc
     4381 agtacctgga ggcgaacccg gaggcgcgcg aggcgatcga acgggagatg ggggattgag
     4441 cgcgtcatga gccgcccgtg ggagaccctg gacgccgccg agacccggga ggggctgctg
     4501 gagctgcgcc ggcggggaga cgaattcgtc atcctgatcg gcgggcgggt gctgatgaac
     4561 agcgcgctgc acctcaccga ggacgcggtg gccgagcggg cctgccgcga ggtcgccggc
     4621 cggccccgcc cgcgcgtcct ccgcgtcctc atcggtggcc tgggcatggg ctacacgctg
     4681 cgcgccgccc tcgacgctct ccctggctcc gcccgggtgc tggtggccga gatcgatccg
     4741 gtgatcgtcc gctggtgtca ggggccgctc gcccatctca ccggccgggc gctcgaagac
     4801 aagcgcgtgc aggtcgaggt cgccgacgtc ggtgccgtga tcgccacggc ggccaaggag
     4861 ggggcggcgg acccgcggcg cctgttcgac gccatcgtcc tcgacctcta tgaaggaccg
     4921 cgggaggccc atggcgggca gggggacccg ctctacggcc ggcgggcact ggagcgggcg
     4981 cgggcggcgc tcgccccggg cggcgtcctc tccgtctggt ccgaggacgc cgacgccgcc
     5041 ttcgccgagc ggctgcggac ggcgggcttc gaggtgcagc ggctgcggcc gggcaagggg
     5101 gggccgcgcc acgtggtcta cctggcgcgc gcctcctcca gcgccgccgc cagccgcgcc
     5161 acgccgtcgc ggatggccgg cgggtcgacc ggcgcgaagc cgaggagcaa ggcgtcgcgg
     5221 cggggcgcct cccggccgtc cagaccgccg tccagatccc ccagacagta gagggagagc
     5281 ggcgagacct cgaccccgcg ggccgctgcc gcggcggcgg ccttctggtc gtccagcccc
     5341 ggagccaggt cccccagggc gtggaggccg gtgccgccgg aggtcacccg catcaacccg
     5401 ccgcagcgcc gctccgcctc ctcgatcagg cactcgagcc gctcctggta gaggccgcgc
     5461 atgcgccgca cgtggcgggc gaagtgcccc tcggcgagga agtccgccag caccgcctgc
     5521 tccagggagg gcgggtactg ctccgccgcc ccgcgggcgg cgacgaacgg ctccaccagc
     5581 tcctcgggga tcaccaggaa gcccagccgc agcgccggcg ccagcgtctt gctgaaggtg
     5641 ccgatgtaga tcacccggcc ggcggtgtcc agcccctgga gcgcggagat cggccggccg
     5701 gcgtaccggt actcgctgtc gtagtcgtcc tccaggatcc aggcaccccg ctcgcgcgcc
     5761 cagtcgagca gggccagccg gcgggtcagg ctcatcgtga cgccgagcgg aaactggtga
     5821 gagggcgaca cggcggcgag gcgcg
//
LOCUS       DASPJI010000775         5067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_505788, whole genome shotgun sequence.
ACCESSION   DASPJI010000775 DASPJI010000000
VERSION     DASPJI010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505788"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VEL74_21685"
     CDS             <1..405
                     /locus_tag="VEL74_21685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65207.1"
                     /translation="PAALGVSVALGAALSLPSLLPGGEVRDPAFVAASLVAVLCREAS
                     FGLLFTRGGGLLVAGLYRGALYFAETFVINDWYSVYFPAFNYVAGGSALYVARAVAAV
                     LALGVIAAVTGLLRRRSGHAGAMDSIEEEPAA"
     gene            402..854
                     /locus_tag="VEL74_21690"
     CDS             402..854
                     /locus_tag="VEL74_21690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65208.1"
                     /translation="MSEDRPEARGWITFCVLGLVVMATWTLVVKFLVPLLWYIAERSA
                     GRFVGELPVMWDFWWVAHLVLAWLLWRRHRWAWAFGLLVAVIEIAIVITKFAFYLRQP
                     DLGFWRLLWFTNKVYVLAFFVLFVALLARRDVRNALGAGHVPKEEGSA"
     gene            826..2049
                     /locus_tag="VEL74_21695"
     CDS             826..2049
                     /locus_tag="VEL74_21695"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HYG65209.1"
                     /translation="MSRKKKEAPEAGLSRRDLLRTTAIGTAGTVLAAGGSEAASAPRP
                     AMRYRPLGKTGLRVSEVGFGGYPVDDPDVVRYAVDQGITYFDTSHCYRGGRSEEVIGE
                     GLQGRRDRVVLATKWCPHHVGKPAVKKSFLDILDRSLKRLRTDYVDVLLNHEVGERSD
                     GEGVRRLQNLEMWEAWEAARKAGKARFLGVSGHDPDLMEVLGHAVGTGKLSVLLGRYS
                     FLDYPKQNELIVRAHQKGVAFVAMKTLAGAKGADLDRFRGRQTSFKQAALKWVLSNAR
                     LSNLIISMNSRRQVDEYAGASGQKLAAADHEVLEEYASRFSQEVCRFDGACHDACPGG
                     VRIADILRFSMYCHEYEQEGRAMESYAKLVASERAAHCAHCAGFCEQACSYDLPVRTL
                     LIKAHGALGEPPREG"
     gene            2117..2857
                     /locus_tag="VEL74_21700"
     CDS             2117..2857
                     /locus_tag="VEL74_21700"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HYG65210.1"
                     /translation="MSETLFRTMADPIRLRVLSYNVQGHGRLFLRRYLERIARAISEA
                     EPDVVGLQEVHRHTWHSRFEDQAEILARLTGMDVHFGRSFAPEKGGEFGNAVLTRRPV
                     RSSEVHLLPGTGEPRTLLHTRLDLAGFELDFYVTHLAAWGRWGRAARSMQIGGLVERL
                     RQRSEPFVLVGDFNAVPGAPEVKALMAERIFRVCGEEMACTHRIMRQRIDYVLADPGW
                     TTTDARVLQAGPSDHWPVLVELHRETSS"
     gene            2903..4261
                     /locus_tag="VEL74_21705"
     CDS             2903..4261
                     /locus_tag="VEL74_21705"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HYG65211.1"
                     /translation="MAVPAKRPRSPDIPDEKPPRGGKLLPWYRRVLRVSYLGVALYIF
                     VLVGLALILWSTRRERESHVRVPDIADFEQALPSIANLTGSPVIAGNRVEVLQDGEGF
                     FPVLLADIAAARESVHLETYVWWEGEICRQVARALADKARQGVEVRLLLDAVGSKKGD
                     DELFEEIRKAGGKVEKFHPFSLADIGLINNRTHRKVAILDGRVGHVFGHGIAQEWTGR
                     AQDEKHWRDTGVRLEGPVVNAVQAVFAENWVEQSEEVLVGEKYFPALPAAGSVRAHVT
                     ASSPQGGVSRLEMLLKLAIVTAQEELLIQNPYFIPDGEMVGLMEKAVERGVDVRVMVP
                     GVVTDSSVVRHAGHRRFEELLDHGVKICEYQRTLNHQKVMVVDGIWSLVGSTNLDDRS
                     LDINDEASVGLIDRGVAGELRAAFAADARHCRPITPETWGRRSAWHKLVDRVSYLLNE
                     QL"
     gene            4282..>5067
                     /locus_tag="VEL74_21710"
     CDS             4282..>5067
                     /locus_tag="VEL74_21710"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HYG65212.1"
                     /translation="MVEVPIWVLVTMSLALVLVGTMLWTLLRERRFRVRIPDLDCFEQ
                     ALPSIAGMTQATILQGNSVDLLQNGDEFFPAFLSAMKEARETIHFETYVWWDGDICGE
                     VAETLAERARAGLEVRVVIDAVGSLKMDKRLIKVMRGAGVKVTRYHKLRLKDIGQLNK
                     RTHRKLAIFDGKMAFVMGHGMAKEWLGHGQDKDHWRDTGVRLHGPIVNAVQSVFAQHW
                     VEETGEVLVGEKYFPHLEEAGKIPIHMLAGAPLGGVSGLELMFK"
BASE COUNT          773 a         1661 c         1809 g          824 t
ORIGIN      
        1 ccggccgcgc tcggcgtctc ggtggcgttg ggggcggccc tctcgctgcc gtccctcctg
       61 ccgggcggcg aggtacggga cccggcgttc gtcgccgcgt cgctggtggc ggtcctctgc
      121 cgcgaggcct ccttcgggct gctgttcacc cgcggcggcg gcctgctggt agcggggctc
      181 taccgcggcg ccctctactt cgccgagacc ttcgtcatca atgactggta cagcgtctac
      241 ttcccggcct tcaactatgt ggcggggggc tcggcgctct acgtcgcgcg ggcggtggcg
      301 gcggtcctcg ccctcggcgt gatcgccgcc gtcaccgggc tcctccggcg ccgtagcggc
      361 cacgcaggag ccatggactc tattgaagag gagcccgcgg catgagcgaa gaccggccgg
      421 aggcgcgggg ctggatcacc ttctgcgtcc tcgggctggt cgtgatggcc acgtggacgc
      481 tggtggtcaa gttcctcgtg cccctccttt ggtacatcgc cgagcgatcg gctggccgct
      541 tcgtgggcga gctgcccgtc atgtgggact tctggtgggt ggcccacctg gtgctggcct
      601 ggctcctctg gcgccgccac cgctgggcct gggccttcgg cctgctggtg gcggtcatcg
      661 agatcgccat cgtgatcacc aagttcgcct tctatctgcg gcagccggac ctcgggttct
      721 ggcgtctgct ctggttcacc aacaaggtct acgtgctggc gttcttcgtc ctcttcgtgg
      781 ccctgctagc caggcgggac gtgcggaacg ccctgggagc cggtcatgtc ccgaaagaag
      841 aaggaagcgc ctgaggcggg cctttcccgc cgcgacctgc tgcgcaccac cgcgatcggc
      901 acggcgggga ccgtccttgc cgccggcggg tcagaggcgg cgtccgcgcc ccggccggcg
      961 atgcgctacc ggcccctggg caagaccggc ctgcgggtct ccgaggtcgg cttcggcggc
     1021 tatccggtgg acgacccgga cgtggtgcgc tacgccgtgg atcagggcat cacctacttc
     1081 gacacctccc actgctaccg cggcgggcgg agcgaggagg tcatcggcga agggctccag
     1141 ggacggcgcg accgggtggt gctggccacc aagtggtgcc cgcaccacgt gggaaagccc
     1201 gcggtcaaga agagctttct cgacatcctg gaccgtagcc tcaagcgcct gcggaccgac
     1261 tacgtcgacg tgctgctcaa ccatgaagtg ggagagcggt cggacggcga gggagtgcgg
     1321 cgtcttcaga acctcgagat gtgggaggcc tgggaggcgg cgcgcaaggc cggcaaggcc
     1381 cgcttcctgg gggtgagcgg tcacgatccg gacctcatgg aggtcctggg gcatgccgtg
     1441 ggcaccggca agctctccgt cctcctgggc cgctacagct tcctggacta cccgaagcag
     1501 aacgagctca tcgtccgcgc ccatcagaaa ggggtggcct tcgtggccat gaagaccctg
     1561 gccggcgcca agggggccga cctcgatcgc ttccgcggcc gccagacgag cttcaagcag
     1621 gcggcgctca agtgggtgct gtcgaacgcc cggctgtcga acctgatcat ctcgatgaac
     1681 agccgccggc aggtggacga gtacgccggc gcgtccggcc agaagctcgc cgccgccgat
     1741 cacgaggttc tggaggagta cgcgtcccgc ttctcccagg aggtctgccg cttcgacggc
     1801 gcctgccacg acgcctgccc ggggggcgtg cggatcgccg acatcctgcg cttctccatg
     1861 tactgccacg agtacgagca ggaggggcgg gcaatggagt cctacgcgaa gctcgtcgcc
     1921 tccgagcgcg ccgcccactg cgcccactgc gccggcttct gcgagcaggc ctgcagctac
     1981 gacctgccgg ttcgaaccct gctgatcaag gcgcatgggg cgttggggga gccgccgcgg
     2041 gaaggctgac gaagaggcat gctgttcgca atgggagaag ggacggagcc atgggaccgg
     2101 ctccggagtg atgaagatgt ccgagaccct cttccggacc atggcggacc ctatccgcct
     2161 gcgcgtcctg tcctacaacg tccaggggca cggcaggctg ttcctgcggc gctacctgga
     2221 gcgcatcgcc cgcgccatca gcgaagcgga gcccgacgtg gtgggcctgc aggaggtcca
     2281 ccgccacacc tggcactccc ggttcgagga ccaggccgag atcctcgccc gcctcaccgg
     2341 catggacgtc cacttcggcc gcagcttcgc gccggagaag gggggcgagt tcggcaacgc
     2401 ggtgctcacc cgccgtccgg tgcgttcgtc cgaggtccat ctgctgcccg gcaccggcga
     2461 gccgcgcacc cttctccaca cccggctcga cctcgccggc ttcgagctcg acttctacgt
     2521 gacccacctc gccgcctggg gacgctgggg ccgcgcggca cggagtatgc aaatcggcgg
     2581 attggtagag cggctcaggc agcgcagcga gcccttcgtg ctggtgggag atttcaacgc
     2641 cgttccgggc gcccccgaag tgaaggccct gatggcggaa cggatcttcc gggtgtgcgg
     2701 agaggagatg gcctgcaccc atcggatcat gcgccagcgg atcgactacg tgttggccga
     2761 tccaggctgg accacgaccg atgcccgggt gctccaggcc ggcccgtcgg accactggcc
     2821 ggtgctcgtt gagctgcaca gagaaacctc ctcgtaagac ccgccggccc tgagcgctct
     2881 catcttccca aggaggagtg ccatggctgt gcctgcaaaa cgtccgcgct cgccggacat
     2941 cccggacgag aaaccacccc gcggggggaa gctgctccca tggtaccggc gcgtcctccg
     3001 ggtgtcgtac ctgggggtgg cgctctacat cttcgtgctg gtggggcttg ccctgatcct
     3061 ctggtccacc cggcgggagc gcgagtccca cgtgcgcgtg ccggacatcg ccgatttcga
     3121 gcaggcgctg ccctcgatcg ccaacctgac gggatcgccg gtgatcgcgg gcaaccgcgt
     3181 cgaggtgctg caggatggcg agggcttctt ccccgtcctg ctcgccgaca ttgcggcggc
     3241 gcgcgagtcg gtccacctgg agacctacgt ctggtgggag ggggagatct gccggcaggt
     3301 ggcgcgggcg ctcgccgaca aggcccgcca gggggtggag gtgcgtctgc tcctcgacgc
     3361 cgtgggctcc aagaagggag acgacgagct cttcgaagag atccgcaagg cggggggcaa
     3421 agtggagaag ttccacccct tcagtctggc cgacatcgga ctgatcaaca accggaccca
     3481 ccggaaggtg gcgatcctcg acggccgcgt ggggcacgtc ttcggccatg gtatcgccca
     3541 ggagtggacc ggccgggccc aggacgagaa gcactggcgc gataccgggg tgcgcctgga
     3601 agggccggtg gtcaacgccg tccaggccgt gttcgcggag aactgggtgg agcagagcga
     3661 ggaggtgctg gtgggggaga agtacttccc cgcgttgccg gcggcgggga gcgtccgcgc
     3721 ccacgtcacg gccagctcgc cgcaaggtgg ggtctcacgg ttggagatgc tcctcaagct
     3781 cgccatcgtc accgcccagg aggagctgct catccagaac ccctacttca tcccggacgg
     3841 cgaaatggtg ggcctcatgg aaaaggccgt ggaacgcggc gtcgacgtcc gcgtcatggt
     3901 gccgggggtg gtgaccgaca gctcggtggt ccgccatgcc gggcaccggc gcttcgagga
     3961 gctgctggac cacggggtga agatctgtga gtaccagcgg acgctcaacc atcagaaggt
     4021 gatggtggtg gacggcatct ggtcgctggt gggctcgacc aatctcgatg accgctcgct
     4081 ggacatcaat gacgaggcca gcgtcggcct catcgaccgt ggagtggcgg gcgagctgcg
     4141 ggcggcgttc gccgcggacg cgcggcactg ccggccgatc actccggaga cctggggccg
     4201 gcgctcggcc tggcacaagc tggtggaccg ggtctcctat ctgctcaacg agcagctctg
     4261 atctgagagg aaggtgaacg gatggtcgaa gtcccgatct gggtcctggt cacgatgtcc
     4321 ctggctctcg tgctcgttgg gaccatgttg tggacactcc tgcgggagcg gcggttccgg
     4381 gtgcgcatcc cggacctgga ctgcttcgag caggccctcc cctccatcgc cgggatgacc
     4441 caggcgacga tcctccaggg caacagcgtc gacctgctgc agaacggcga cgaatttttc
     4501 ccggcgttcc tgtcggcgat gaaggaggcg cgggagacca tccatttcga gacctacgtc
     4561 tggtgggacg gcgacatctg cggcgaggtg gccgagaccc tggccgagcg ggcgcgggcc
     4621 gggctcgagg tgcgggtggt gatcgacgcc gtcggctcgt tgaagatgga caagcggctc
     4681 atcaaggtga tgcggggcgc cggcgtcaag gtgacgcgtt accacaagct ccgcctcaag
     4741 gacatcggcc agctcaacaa gcgcacccat cgcaagctcg ccatcttcga cggcaagatg
     4801 gccttcgtca tggggcacgg catggcaaag gagtggctcg gccatggcca ggacaaggac
     4861 cactggcgcg acaccggagt gcgcctgcat gggccgatcg tcaacgccgt gcagtcggtc
     4921 ttcgcccagc actgggtgga ggagaccggc gaggtgctgg tgggagagaa gtatttcccg
     4981 cacctggagg aggcggggaa gatcccgatc cacatgctgg ccggcgcccc cctgggcggc
     5041 gtctcgggcc tggagctgat gttcaag
//
LOCUS       DASPJI010000776        14435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_108206, whole genome shotgun sequence.
ACCESSION   DASPJI010000776 DASPJI010000000
VERSION     DASPJI010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14435)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14435
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108206"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..2161)
                     /locus_tag="VEL74_21715"
     CDS             complement(218..2161)
                     /locus_tag="VEL74_21715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HYG65213.1"
                     /translation="MTMNETHPLSATDVLRQTFGFDAFRPGQEEVVGHLLAGRSAAAV
                     FPTGAGKSLCFQLPALLLPGLTLVVSPLIALMKDQIDQLKARGIDAERLDSTLSAEEH
                     GAIMSAVRAGSLRLLYVAPERFNNERFREAMRRVRISLFAVDEAHCVSEWGHNFRPDY
                     LKLAAFGRLCGAERVLALTATATPQVLDDICRGFEIDPASAVRTGFYRPNLTLLTTPM
                     EPERRDDELCQRLAHRGEGTGGPAIVYVTQQRTAEEVADILARAGLDARAYHAGLEDD
                     ERSRVQDWFLRSDRAVVVATIAFGMGIDKPDIRTVIHYNLPKSLENLSQEIGRAGRDG
                     LPSRCEIFVSPGDLRVLRNFAYGDTPEPAAVRGLLDEIAAGPDTLELNLQDLSAAHDV
                     RLLVVRTLLTYLEIDGYLEAGTPTWSQYQFQALESSEQILARFEGERRDFLHSLFAQA
                     KKAKTWYHIDLDEAAAALGSPRDRLVRALDWLGEQKLLEVRSAGLRYRYRWLRRPQDL
                     DGLAESLHRRTLEREEREIARLDQVLELAAHDGCQTAFLCAHFGETLPHPCGHCSWCL
                     RGGPMAIPPEPPWRIDEAFWRQAEGVRRERAADLASPRAFARFLTGLPSPRLTRAKLG
                     RHPFFGALSDVPFPEVLRRAEGE"
     gene            2758..3033
                     /locus_tag="VEL74_21720"
     CDS             2758..3033
                     /locus_tag="VEL74_21720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65214.1"
                     /translation="MVHVYFHPTPEEVEQARQDPGNHWVPDYGPDAARLHVFYAYGRW
                     FAVWEALEEKKEAAADPSLPEWRRWSVVRIVEDADPSSATGVTFLEV"
     gene            complement(3804..6152)
                     /locus_tag="VEL74_21725"
     CDS             complement(3804..6152)
                     /locus_tag="VEL74_21725"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HYG65215.1"
                     /translation="MKGLRRTLAWLALACLPGMGSTAYAQSCQTHTGLTYATYVDGGG
                     QTQPLQLELLVPTGAAPPRPVVIWIHGGGWSTGSRLPIPAGVSALCSQGYAVASVDYR
                     LSGPGARWPLQLHDVKGAVRWLRANAATYGLDPDRFAAWGSSAGAQLAIMLGTAANVG
                     PFTFGNVTVDLEGSVGGNPGQSSRVQAVVNWFGQTDFLQMRFFPTPTSDHDAANSPES
                     RLLGGPIQDLPELAATANAMTFASADDPPFLVMHGALDELVPFHQSELLVSALRAHGV
                     RVTFVPVPTAGHGGGGFTTPANHQTVYDFLAAVLAEPPATKVSVQALAPTAAETGAVG
                     SFRVSRTGGTGAPLTVRWDLQGTATLGADYSAPFSATIPAGAASVTIPVVPANDPLVE
                     GNETVVIALAHDPAYGVEAARAQAVVTIADNESAAGLPAVQVAATDAAADEDGDPGAF
                     TVSLDTPAPAAGLTVRYTVSGTALQGVDAAPLAGALTIPAGETSAVLPVLPVDDAVLE
                     GSEALVVTLEAGAGYVREAPASVSVRIADTDFDPAVPVVSIAATDFTASEPGNNTGTF
                     TVTRTGSTAASLVAFIQPGGQAREGSDYLTLPNGVIFDAGVNRVTVTVTPAGDSLAEG
                     AETVTLATDADPDYLPGPQSSAVILADDEITGTAGFYPVRPCRLVDTRGVAGPRGAPA
                     LTCDGAIRVFEVTGACGIPPEATAISANVVAVAPTTTGHLSLFQIGAAVQTSTVNFAA
                     GQTRANNVVLPLSGTPGTLAALCGGAAPGQADLVIDVNGYFR"
     gene            complement(6321..7094)
                     /gene="cobA"
                     /locus_tag="VEL74_21730"
     CDS             complement(6321..7094)
                     /gene="cobA"
                     /locus_tag="VEL74_21730"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015847454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HYG65216.1"
                     /translation="MSAGRVFIVGAGPGDPDLLTVKALKALRRADVVVYDRLVNPEIL
                     AEAPAGAERIFAGKAPGAHSCAQEEINALLVRHAREGRVVVRLKGGDPFVFGRGGEEA
                     LACAEAGVAWEVIPGLTSAVSVPAAAGIPVTHRGIAGAFAVVTGHCMDRDRLDWEALA
                     RIDTLVVLMGVGRLPTLTAQLLRHGRPADTPAAVIEKGTRPGERVLTGTLATLAEDAA
                     RAEIGAPAILVIGEVVRVRESLGLAMDAAALGDVLSAVA"
     gene            complement(7091..7741)
                     /locus_tag="VEL74_21735"
     CDS             complement(7091..7741)
                     /locus_tag="VEL74_21735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HYG65217.1"
                     /translation="MPYYPVYLDLAGRACAVIGGGHLAEEKVRGLLAAGAMVRVVAPE
                     LTPGLAELAAAGGIDHLPRAFEPGDLDGVFLAFTAGEAPATVEAVWDEARRAKVLLNT
                     VDDVPHCDFIMPSILRRGDLTIAISTAGRAPALAVRLRECLEGVVGPEHARFLELAGA
                     VRAPLARRWPDFATRRELWYRLVDSDVLALLRQGDEEAAVARFHEIMDVTPAEAVA"
     gene            complement(7745..8227)
                     /locus_tag="VEL74_21740"
     CDS             complement(7745..8227)
                     /locus_tag="VEL74_21740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65218.1"
                     /translation="MSGAAPTTSASALAVDAAQREVLLEEIAALAAAVRDPESRERYG
                     ALHAAVEAGEVGDDLAGALEGLLEMLLQTGRARRIHGADGEQSLLRLFHKTPRGAAAR
                     RSTEAVNEALRGLRGQTLENLLFTVQGPGVYRLGLATDRCRLTVEIDRHGVSVESLEV
                     "
     gene            complement(8224..9048)
                     /locus_tag="VEL74_21745"
     CDS             complement(8224..9048)
                     /locus_tag="VEL74_21745"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HYG65219.1"
                     /translation="MIDSIAYDQRDLLDEFEAGQLAVELDFATPQEVLQWALERWGSN
                     VALCTSFQAEGMAILDMAWRIDPAIRVFTVDTGRLPQETYDIMEEVREKYGIEIEVCF
                     PDAAKVEGMVRRLGPNLFQRSVEARLLCCSVRKVEPITGMLRGLDAWITGLRREQWAS
                     RANIRKIEVDHDHGGLAKISPLADWTIEEVQEYNREHGVPVHALYAKGYTSIGCAPCT
                     RATKAGEDPRAGRWWWEKNAPKECGIHCPLETGGFEHEVEAILHRGATGPRRVETA"
     gene            complement(9067..10725)
                     /locus_tag="VEL74_21750"
     CDS             complement(9067..10725)
                     /locus_tag="VEL74_21750"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HYG65220.1"
                     /translation="MPEAEPTWELVHKRNFVERLKHEKGGLDVIRDLPAMIETGYEAV
                     PEEDFVRMQWYGLYHDKPKLGHFMLRVKVPGGVLTPAELRIIGGLSERFGRGFGELTT
                     RQNIQLHWLELGHLPEVLSILDQGGLSTVGACGDNVRNITGCPVAGIDGAELFDTREI
                     LTGASGFFASHREYSDLPRKHKITVSACPHQCNAPEIHCIALIGDLRDGRPGFAVRVG
                     GGLSTWPRLADDLGVWVPAAESLDVLRAIVDLWREDLRYRLSRNKSRLKFMAHDLGPE
                     TFRARVEERLGRRLADGRVPLPPEAEHDHAGVHPQRQEGLFYAGFPVHLGLMNGATML
                     AIADLAEPYGGQIRLTRRQNFILSHIPEDRVEDVVFRVKELGFPLEGTGLRGASIACT
                     GDPFCNYTVAETKGKLEEIVRHLEEVFGQEASGLRLNLDGCPHACAHHWIGDIGFQGT
                     TLRERGPQGEKVHGYDLFLRGGLARNAAIGRPVLKRVPGTEVHLVVERLFRAYLEERA
                     DGEPIQQFFTRKTDEELLALGTGSPTIDSADPTDSVGAAVGGLS"
     gene            complement(11078..12817)
                     /locus_tag="VEL74_21755"
     CDS             complement(11078..12817)
                     /locus_tag="VEL74_21755"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HYG65221.1"
                     /translation="MRDRTRRSVAFALALALLASPWRPAAAQTIANPDLFAKSLTVAQ
                     EAVEQYGLYDNSEELARVNRIGYELAQHSEFQKFPFTFGLVDVPVPNAFALPGGQIFV
                     TRGMLDLGLDDDMLANVLGHEIAHVTLEHYLRMKRKATLMSVLGNLLVAGILVGEANS
                     NNRTRDEAPYDPRVGRDPGGDLIQGAAAASLILSELLLRSHSREHEDESDKEGQRLAA
                     SAGYDPDGARRLWEKMESRAPQFKQYGYWQTHPFAVERSKAAGARKGTWKVQTGKSAD
                     DYRRRTQAVLTNYLASHRPTEGETAFLKSAALTAWPQGKTAEEYRLEKLHRQRDQEMA
                     KPLLSRDYGAVVRAYRDEMKELKALDPGSDLLRTLQADIDDLESKRKELFRRAVEVLD
                     GGVYETSFLEAFLSNFPDASQVPEVALALGDAYSRLGNQTDGVTQYLAAFQSGPDTAE
                     GKRARTGLRNLTPNLKELSALQQLIDQNDDPELKRLAADRLAQVVKSYEDVANGAEYL
                     RRFPEGPHVPAVMDRLNSLADNLYGEVVLYQSVGDDVKAIERINKILTHAPLSPAAEK
                     LRDRAQIPTEKAG"
     gene            complement(12887..13633)
                     /locus_tag="VEL74_21760"
     CDS             complement(12887..13633)
                     /locus_tag="VEL74_21760"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HYG65222.1"
                     /translation="MSAVPGLEPPAHPHSPSWRHRFRTVLLAGLLFVAPVFLTIYVLR
                     VLFLFMDGIFAPLIDRTLRQVFPESAIHIPGLGILLTLAVVLFLGWLSTNLVGRRLLD
                     SVEAFFLRVPVVKSVYGATKGVLEAVSHDQAEAFKRVVLVEYPRLGVFSIGFVTGAPS
                     RWPMADPRADEELVPVLIPRTPNPLSGYIVLLPPRQLIECAITVEEGVRMVVSGGILP
                     PESIQSQLRSPRSPLPEAAEKELEDAALPR"
     gene            13697..13975
                     /locus_tag="VEL74_21765"
     CDS             13697..13975
                     /locus_tag="VEL74_21765"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HYG65223.1"
                     /translation="MSDLTIEVETAKSSMEEIRPTLDAGLKEEFPGGMLKWRWEGDVM
                     HLSGPGASATIVYEPGKLVGRAHLKPPASLMKPVIQQKISSVMKKAAV"
     gene            13993..>14435
                     /locus_tag="VEL74_21770"
     CDS             13993..>14435
                     /locus_tag="VEL74_21770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65224.1"
                     /translation="MAILPGALLRHAAERPEVPWLFWAEGWDWRWLSWGEAARRVMAE
                     ADTFAGGPPLVPSPDPLTPAALITDLASQAAGAVVRVRGSGDEWQEWGAEDLGAAADA
                     VAREVAPAGPPAGGREILVAGRPLAEPAERAVVAWAILQGAALLL"
BASE COUNT         2066 a         4933 c         5310 g         2126 t
ORIGIN      
        1 acttcaaggc gaccgggcgc tccagcacca ggtcctcggc cttgtacacc acccccatcc
       61 cgccctcgcc cagcttctcc agcagccggt agtgcgagac ggtcttaccg atccaggagt
      121 cctggagaac tttagcgggc atgactgtcg ccgggaacct cggagagcat cctatcttag
      181 aagacgtgaa cctgcgacat gtctcccgtt catcctttca ttcaccctcc gcgcgccgca
      241 gcacctccgg gaacggcacg tcggagagcg cgccgaagaa ggggtgcctc cccagcttcg
      301 cgcgggtgag gcgcggggag gggaggccgg tgaggaagcg ggcgaaggcg cggggggagg
      361 cgaggtccgc ggctcgctcg cgccgcaccc cctcggcctg gcgccagaag gcctcgtcga
      421 tcctccacgg cggctccggc gggatcgcca tcgggccgcc gcgcaggcac caggagcagt
      481 ggccgcacgg gtgggggagg gtctcgccga aatgggcgca gaggaaggcg gtctggcagc
      541 cgtcgtgggc ggcgagctcc agcacctggt cgaggcgggc gatctcccgc tcctcgcgct
      601 ccagggtgcg gcgatggagc gactccgcca gcccgtccag gtcctgcggc cggcgcagcc
      661 agcgataacg gtagcgcagg ccggcgctcc tcacctccag gagcttctgc tcgcccagcc
      721 agtcgagcgc ccgcaccagc cggtcgcgcg gggagcccag ggccgcggcg gcctcgtcga
      781 ggtcgatgtg gtaccaggtc ttggccttct tcgcctgggc aaagagggag tgcaggaagt
      841 cccgccgctc cccctcgaag cgggcgagga tctgctcgga gctctccagc gcctggaact
      901 ggtattgcga ccaggtcggg gtgccggcct cgagatagcc gtcgatctcc agataggtga
      961 ggagcgtccg caccaccagc aggcggacgt cgtgcgcggc cgagaggtcc tggaggttga
     1021 gctccagggt gtcgggcccg gcggcgatct cgtcgaggag gccgcgcacc gccgccggct
     1081 ccggcgtgtc gccgtaggcg aagttgcgga gcacccgcag gtcgccgggg gagacgaaga
     1141 tctcgcaccg ggaggggagg ccgtcgcgac cggcgcgacc gatctcctgc gagaggttct
     1201 ccaggctctt cggcaggttg tagtggatca ccgtgcggat gtccggcttg tcgatcccca
     1261 tgccgaaggc gatggtcgcc accaccaccg cgcggtcgga gcgcaggaac cagtcctgga
     1321 cccgggagcg ctcgtcgtcc tcgaggcccg cgtgataggc gcgggcgtcc aggccggccc
     1381 gggcgaggat gtccgccacc tcttcggcgg tccgctgctg ggtgacatag acgatggccg
     1441 gcccccccgt gccttcgcca cgatgcgcca ggcgctggca gagctcgtcg tcccgccgct
     1501 cgggttccat gggggtggtg agcagggtga ggttgggccg gtagaagccg gtgcggacgg
     1561 cggaggccgg gtcgatctcg aagccgcggc agatgtcgtc caggacctgg ggcgtggcgg
     1621 tggcggtgag ggccaggacg cgctcggcgc cgcagaggcg gccgaaggcg gcgagcttca
     1681 ggtagtccgg ccggaagttg tggccccact ccgagacgca gtgcgcctcg tccaccgcga
     1741 acagggagat gcggacccgc cgcatcgcct cgcggaagcg ctcgttgttg aagcgctccg
     1801 gcgccacgta caggagccgg agggagccgg cgcggaccgc cgacatgatg gcgccgtgct
     1861 cctcggccga cagcgtcgag tcgagccgct cggcgtcgat cccccgcgcc ttgagctggt
     1921 cgatctggtc cttcatcagg gcgatgagcg gcgagaccac cagggtgagg cccggcagga
     1981 gcagggccgg gagctggaag cagagcgact tgccggcgcc ggtggggaag acggcggcgg
     2041 ccgagcgccc ggccagcagg tggccgacga cctcctcctg accgggacgg aaggcgtcga
     2101 agccgaaggt ctggcggagc acgtcggtgg cagacagggg atgagtctcg ttcatggtca
     2161 tgggtatctt cctcctctgt caggaggaaa gacgtaaaac gccggaaaaa tcttgcagaa
     2221 atctttggat ggggtcaggg gagcaggaat tgggacgtct acccagagag tcctcacccc
     2281 cccaaccccc tctcccatct cccacccacc ccccggccgg gagaggggga gcaagaatcc
     2341 agtggccgag ggggcggtgg tgcctcccgg ccgcttcggg tcaggagggg ggtttttcac
     2401 acccctccgc actccgcaac tttcccgcaa gaatcccgca tttccctctt gacgcatccc
     2461 cggtcgcatt atagtcttgg gcggtcgtaa gccgtacaac agaagggagg tggtagggga
     2521 gagaacgaag ccacggtttc acagcacagc cgtacaggac cgttgtgtct ccggggtgag
     2581 cgtgtggctc atccggggct ccagggtcca agcggccaag ggagggagcg ggaactcgcc
     2641 tcccgaggcc ttgccgagca gaggagataa ctcacgcccg accacggtga tcgtacgcct
     2701 tcgcgcgtcg atctgtcccg tcccaccgaa tccacccgcc gtcatctcta cgagctggtg
     2761 gtccacgtct acttccatcc cactcctgaa gaggtcgagc aagcccgcca ggatcccggc
     2821 aaccactggg tgccggacta cggtcccgac gcggcgcgcc tccacgtctt ctacgcctac
     2881 ggccgctggt tcgcggtctg ggaggcgctg gaggagaaga aggaggcggc ggccgatccg
     2941 tcgctccccg agtggcggcg ctggtcggtg gtgcggatcg tcgaggacgc agacccgtcg
     3001 agcgcgacgg gcgtgacctt tctcgaggtc tgacgccgtt caggcggcgg gcctcgcgtc
     3061 gcggggcccg ccccctctcc ccctgacgac aaggaagaga tccccgccgc cgcggaagcg
     3121 gcggcgggga tcattcccca tggggcagcc gctgccccgg gaggaatggc catcattcct
     3181 gtcggggcaa tcgttgcccc acgaggaatg gtgtgcgttc ctgtcggggc gatcgctgcc
     3241 ccatgaggaa tggaggtcat tcctgtcggg gcagccgctg ccccatcagg aatggagatc
     3301 attcctatcg gggcaatcgc tgccccatca ggaacggcgg ctgtttctgc tggggcgatg
     3361 gttgccccac gaggaaggac cgccatttct catcgggcaa cggttgcccc acgagggatg
     3421 ggcctcattt ttcggcggcg ggtggcgtcc ttgctcggtg tggggggtgg gaggggagcg
     3481 gcggagccgc tccccgaaag cgacctcaac agccctggca gcttaccttc tgcgcggcgg
     3541 tctggcagtt agttggcccc gtggccccca ctactacctc tagattctcc cgctccaggt
     3601 cccgcagcgt ctccctggac agcaccagtt tcttcgccgt cttcttcatc gccactcctt
     3661 agtaggttcg cgccacctgg cgcgagggca ttgtagccct tggggacctg ttactgcttt
     3721 gggcagctcg gcggttcggg aagattgcct gcgccctttc agggctggga gggtggggtg
     3781 ggggtgtact tcctccatac tcctcaacgg aagtagccgt tgacgtcgat caccaggtcg
     3841 gcctggcccg gcgcggcgcc gccgcagagt gcggcgaggg tgccgggggt gccggagagg
     3901 gggaggacga cgttgttggc ccgcgtctgg ccggcggcga agttcaccgt cgaggtctgc
     3961 accgcggcgc cgatctggaa caggctcagg tggcccgtcg tcgtgggcgc cacggcgacc
     4021 acgttcgccg agatcgccgt cgcctcgggg gggatgccgc aggcgcccgt cacctcgaag
     4081 acccggatcg cgccgtcgca ggtcagggcg ggcgcacccc gcggccccgc cacgccgcgg
     4141 gtgtccacca gccggcaggg gcggaccggg tagaagccgg cagtccccgt gatctcgtcg
     4201 tcggcgagga tcacggcgct cgactgcggg cccggcaggt agtcggggtc ggcgtccgtc
     4261 gccagcgtca ccgtctccgc cccctcggcc agcgagtcgc cggccggggt cacggtcacc
     4321 gtcacccggt tgacgccggc atcgaagatc acgccgttcg gcagggtgag atagtcggac
     4381 ccctcccgcg cctggccgcc gggctggatg aaggcgacga gactcgccgc ggtgctcccc
     4441 gtgcgcgtca ccgtgaaggt cccggtgttg ttgcccggct cggatgccgt gaagtccgtt
     4501 gccgcgatgg agaccaccgg cacggcgggg tcgaaatcgg tgtcggcgat ccgcacgctc
     4561 accgaagccg gcgcttcccg gacatagccg gccccggcct ccagggtcac gaccagggcc
     4621 tcggaccctt cgaggaccgc gtcgtccacc ggcaggaccg ggaggacggc ggaggtctca
     4681 cccgccggga tggtcagggc gccggcgaga ggggcggcgt ccaccccctg gagggccgtg
     4741 ccggaaacgg tgtagcgcac cgtcaggccg gccgccggcg cgggcgtgtc gagggagacc
     4801 gtgaaggcgc cgggatctcc atcctcatcg gcggccgcgt ccgtggcggc cacctggacg
     4861 gcggggaggc cggctgcgga ttcgttgtcg gcgatcgtca ccaccgcctg cgcccgcgcc
     4921 gcctccaccc cgtaggccgg gtcgtgcgcc agggcgatca ccaccgtttc gttcccctcc
     4981 accagcggat cgttcgccgg cacgaccgga atcgtcaccg aggccgcccc cgccgggatc
     5041 gtggcggaga agggggcgct gtaatcggcg cccagggtgg cggtcccctg gaggtcccag
     5101 cgcacggtga gcggcgcccc ggtgccgccg gtccgcgaca cccggaagct ccccacggct
     5161 cccgtctcgg ccgccgtggg ggcgagcgcc tgcacgctca ccttggtggc ggggggctcc
     5221 gccagcaccg cggcgaggaa gtcgtagacg gtctggtgat tggccggcgt cgtgaagccg
     5281 ccgccgccgt gcccggcagt cgggaccggc acgaaggtga cccgcacccc gtgcgcccgc
     5341 agggcggaga ccagcagctc gctctgatgg aaggggacca gctcgtccag ggcgccgtgc
     5401 atcacgagaa aaggcggatc gtcggccgac gcgaacgtca tggcgttggc cgtggccgcc
     5461 agctccggga ggtcctggat gggcccgccg aggagccggc tctccgggga gttggcggcg
     5521 tcgtggtcgc tggtcggggt ggggaagaag cgcatctgga ggaaatcggt ctggccgaac
     5581 cagttcacca ccgcctgcac ccggctcgac tgcccggggt tcccccctac gctcccttcg
     5641 aggtccaccg tgacgttgcc gaaggtgaac gggccgacat tggcggcggt ccccagcatg
     5701 atggcgagct gggcgccggc cgaggagccc caggcggcga agcggtccgg gtcgaggccg
     5761 taggtggcgg cgttggcccg cagccagcgc accgccccct tgacgtcatg gagctgcagc
     5821 ggccagcgcg cccccggccc ggagaggcga tagtcgacgc tcgccacggc atagccctgg
     5881 gagcagagcg ccgagacccc ggccgggatc ggcaggcggc tgccggtgga ccatccgccg
     5941 ccgtggatcc agatcaccac cggccggggc ggcgcggccc cggtggggac cagcagctcc
     6001 aactggagcg gctgggtctg tccgccacca tctacatagg tggcataggt caggcccgtg
     6061 tgagtctggc agctctgggc ataggcggtg gatcccatgc ccggcaggca ggccagggcc
     6121 agccaggcca gggtacgccg cagtcctttc attctggatt cgctccggtc catcgcactg
     6181 ccacaacgga gcgcgggaat ctgcagaaat cgtgccgtcc atggggtggt gttgaaggaa
     6241 agcgagagca acgcccgatc ggcgggcggt ggggccgggc gctctgggag gaggacggct
     6301 gagggaaggc ggagagcggc tcaggccacg gcgctgagga catcgccgag agccgcggcg
     6361 tccatcgcca ggcccagact ctcccgcacc cgtaccacct cgccgatgac gaggatggcc
     6421 ggggcgccga tctcggcgcg ggcggcgtcc tcggcgagag tggcgagggt gccggtgagg
     6481 acccgctcgc ccgggcgcgt ccccttctcg ataacggcgg ccggggtgtc cgcgggccgg
     6541 ccgtggcgga ggagctgggc ggtgagggtg gggaggcggc ccacgcccat caggacgacc
     6601 agggtatcga tgcgggcgag ggcctcccag tcgaggcggt cccggtccat gcagtggccg
     6661 gtgacgacgg cgaaggcgcc ggcgatgccg cggtgggtca ccgggatgcc cgcggcggcc
     6721 gggacgctga cggcgctggt gaggccgggg atcacctccc acgccacccc ggcctcggcg
     6781 caggccaggg cctcctcgcc gccgcggccg aagacgaacg ggtcaccccc cttgaggcgc
     6841 accaccaccc gcccctcgcg ggcgtggcgc accagcagcg cgttgatctc ctcctgcgcg
     6901 caggagtggg cgccgggggc cttgccggcg aagatccgct ccgccccggc cggcgcctcg
     6961 gccaggatct ccggattcac cagccggtca tagaccacta cgtcggcgcg gcgcagggcc
     7021 ttgagcgcct tgacggtcag caagtcggga tcgcccgggc cggctccgac gatgaagacc
     7081 cgcccggcgc tcacgcgaca gcctccgccg gcgtcacgtc catgatctcg tggaagcggg
     7141 cgacggcggc ctcttcatcc ccctggcgca ggagggccag gacgtcggag tccaccagcc
     7201 ggtaccagag ctcgcgccgg gtggcgaagt cgggccagcg gcgggcgagc ggggcgcgga
     7261 ccgctcccgc cagctccagg aagcgggcgt gctccggacc gaccaccccc tccaggcact
     7321 cgcgcaggcg gacggcgaga gcgggggccc ggccggccgt cgagatggcg atcgtcaggt
     7381 cgccgcgccg caggatggac ggcatgatga agtcgcagtg cgggacgtcg tccaccgtgt
     7441 tgagcaggac cttggcgcgc cgcgcctcgt cccagaccgc ctccaccgtg gccggggcct
     7501 cgccggccgt gaatgcgagg aagacgccgt cgaggtcgcc gggctcgaag gcgcggggga
     7561 ggtggtcgat gccgccggcg gcggccagct cggcgaggcc cggggtgagc tcgggcgcca
     7621 ccacgcggac catcgccccg gccgccagca gcccccggac cttctcctcg gccaggtgcc
     7681 cgccgccgat cacggcgcag gcgcggcccg cgagatccag gtagacgggg tagtagggca
     7741 cggctcagac ctcgaggctc tcgacggaga cgccgtggcg gtcgatctcg accgtgagcc
     7801 ggcagcggtc ggtggcgagg cccagccggt agacgccggg cccctggacg gtgaagagga
     7861 ggttttcgag ggtctggccg cgcaagccgc ggagcgcctc gttgaccgcc tccgtggagc
     7921 ggcgggccgc cgcgccgcgg ggcgtcttgt ggaagaggcg gaggagcgac tgctcgccgt
     7981 cggcgccgtg gatgcgccgc gcccggccgg tctggaggag catctccagc agcccctcga
     8041 gagcgccggc gagatcgtcg ccgacctcgc cggcctcgac cgccgcgtgc agcgcgccgt
     8101 agcgctcccg cgactccgga tcgcggaccg cggcggcgag ggcggcgatc tcctccagga
     8161 ggacctcccg ctgcgccgcg tcgacggcga gagcggaggc cgaagtggtg ggagcggcgc
     8221 cgctcacgcc gtctccactc tccgggggcc ggtggcgccg cggtggagga tggcctccac
     8281 ctcgtgctcg aagccgccgg tctccagcgg gcagtggatg ccgcactcct tgggggcgtt
     8341 cttctcccac caccagcggc cggcgcgggg gtcctcaccc gccttggtgg cgcgggtgca
     8401 cggggcgcag ccgatgctgg tgtagccctt ggcatacagc gcatggacgg ggacgccgtg
     8461 ctcccggttg tactcctgga cctcctcgat ggtccagtcg gccagcgggc tgatcttggc
     8521 gaggccgccg tggtcgtggt cgacctctat cttgcggatg ttggcccggc tcgcccactg
     8581 ctcgcggcgc aggccggtga tccaggcgtc cagcccccgc agcatgccgg tgatgggctc
     8641 gaccttgcgc acgctgcagc agagcaggcg cgcctcgacc gagcgctgga agaggttggg
     8701 gcccaggcgg cgcaccatgc cctccacctt ggcggcgtcg gggaagcaga cctcgatctc
     8761 gatgccgtac ttctcccgga cctcctccat gatgtcgtag gtctcctggg gcaggcggcc
     8821 ggtgtccacc gtgaagaccc ggatcgccgg atcgatccgc caggccatgt ccaggatggc
     8881 catgccctcg gcctggaagc tggtacagag ggcgacgttg gagccccagc gctcgagggc
     8941 ccactggagg acctcctgcg gggtcgcgaa atcgagctcc accgcgagct gccccgcctc
     9001 gaactcgtcg agcaggtccc tttggtcgta ggcgatgctg tcgatcatct tcgtctcctc
     9061 gtgttctcag ctcaggccgc cgactgcggc tccgacggaa tcagtgggat cagccgaatc
     9121 gatggttggg gagccggtgc ccagggccag cagctcctcg tcggtcttgc gggtgaagaa
     9181 ctgctggatc ggctccccgt cggcgcgctc ttccaggtag gcccggaaca ggcgctcgac
     9241 caccaggtgc acctccgtcc ccggcacccg cttgagcacc ggccggccga tggccgcgtt
     9301 ccgggccagg ccgccgcgca ggaagaggtc gtagccgtgg accttctcgc cctgggggcc
     9361 gcgctcccgc agcgtcgtcc cctggaagcc gatgtcgccg atccagtggt gcgcgcaggc
     9421 gtgcgggcag ccgtccaggt tgaggcgcag ccccgacgcc tcctggccga agacctcctc
     9481 caggtgccgg acgatctcct ccagcttccc cttggtctcg gccaccgtgt agttgcagaa
     9541 cggatcgccg gtgcaggcga tgctggcgcc gcgcaggccg gtcccctcga gcgggaagcc
     9601 cagctccttc acccggaaca ccacgtcctc cacccggtcc tcgggaatgt ggctgaggat
     9661 gaagttctgc cgccgtgtca gccggatctg gccgccgtag ggctccgcca ggtcggcgat
     9721 ggccagcatc gtggcgccgt tcatcagccc caggtggacc gggaagccgg catagaacag
     9781 cccctcctgg cgctgcggat ggacgccggc gtggtcgtgc tcggcttccg gcggcagcgg
     9841 cacccggccg tccgccaggc gccggcccag ccgctcctcg acgcgggccc ggaacgtctc
     9901 cggccccagg tcgtgggcca tgaacttgag ccgcgacttg ttgcgcgaca gccggtagcg
     9961 caggtcctcg cgccagaggt cgacgatggc gcgcaggacg tcgagcgact ccgcggccgg
    10021 cacccagacc cccaggtcgt cggccaggcg cggccaggtg gagaggccgc cccccacccg
    10081 caccgcgaag ccgggacggc cgtcgcgcag gtcgccgatc agggcgatgc agtggatctc
    10141 cggcgcgttg cactggtggg ggcaggccga gacggtgatc ttgtgtttgc gcggcaggtc
    10201 cgagtactcg cgatggctgg cgaagaagcc ggacgcgccg gtgaggatct cccgcgtgtc
    10261 gaacagctcg gcgccgtcga tccccgccac cgggcagccg gtgatgttcc ggacgttgtc
    10321 gccgcaggca ccgacggtgc tcaagccccc ctggtccagg atggagagca cctcgggcag
    10381 atggcccagc tccagccagt ggagctggat gttctggcgc gtggtcagct cgccgaagcc
    10441 gcggccgaac cgctccgaca ggccaccgat gatccgcagc tccgccggcg tcagcacgcc
    10501 cccggggacc ttgacccgca gcatgaagtg gccgagcttg ggcttgtcgt ggtagagccc
    10561 gtaccactgc atgcgcacga agtcctcctc ggggactgcc tcgtagccgg tctcgatcat
    10621 cgcggggagg tcgcggatca cgtcgagccc acccttctcg tgcttcagcc gttccacgaa
    10681 gttcctcttg tggaccagct cccaggtggg ttcggcttcg ggcattcggc tcaagacacc
    10741 atctcctttc cttccgcgga ggaaggccgt gccgccggat accggcagcc atccagtttg
    10801 tcggcctgag atcggcgagc gggccctgag caccggcagc cggatccgcc cactaaaggg
    10861 cggatcgctg ccgcgcgctc actcccgctg gctacagagg atggaggagg tgttggtcgc
    10921 cgggctcatc agagacatac ctcataattg tgaccaactt actacagatc agctcgcgtg
    10981 tccagagctt ctcatgcata tcctggaacg aagagggcat gccgttctcc tcacccccgg
    11041 tgtgggggga ggagaacggc gccggaggca gggccggtca gcccgccttt tcggtcggga
    11101 tctgggcgcg gtcgcgcagc ttctcggcgg cgggggagag gggggcgtgg gtcaggatct
    11161 tgttgatccg ctcgatggct ttgacgtcgt cgccgacgct ctggtagagg accacctcgc
    11221 catagaggtt gtcggcgagg gagttcaggc ggtccatgac cgcggggacg tgcggcccct
    11281 cggggaagcg gcgcaggtac tcggcgccgt tggcgacgtc ctcgtagctc ttgaccacct
    11341 gggccaggcg gtcggcggcg aggcgcttga gctccgggtc gtcgttctgg tcgatgagct
    11401 gctgcagggc ggacagctcc ttgaggttcg gcgtcaggtt gcgcaggccg gtgcgtgccc
    11461 gcttgccctc ggcggtgtcg ggcccggact ggaaggcggc caggtactgg gtgacgccgt
    11521 cggtctggtt accgagccgg ctgtaggcgt cgcccagggc gagggcgacc tcggggacct
    11581 gggaggcgtc ggggaagttg ctgaggaagg cctcgaggaa agaggtctcg tagacgccgc
    11641 cgtccaggac ctcgacggcg cgccggaaga gctccttgcg cttgctctcc agatcgtcga
    11701 tgtcggcctg gagggtacga aggaggtcgc tgccgggatc gagagccttc aactccttca
    11761 tctcgtcgcg gtaggcgcgg accacggcgc cgtagtcgcg ggagaggagg ggcttggcca
    11821 tctcctggtc gcgctgccgg tgcagcttct ccagccggta ctcttcggcg gtcttgccct
    11881 gcggccaggc ggtgagggcg gcgctcttca agaacgccgt ctccccctcg gtcgggcggt
    11941 ggctcgccag gtagttggtg agcaccgcct gggtccgtcg ccggtagtcg tcggcgctct
    12001 tgcccgtctg taccttccag gtgcccttgc gggcccccgc cgccttggag cgctccacgg
    12061 cgaaggggtg ggtctgccag tagccgtact gcttgaactg cggggcgcgg ctctccatct
    12121 tctcccacag gcggcgggcg ccgtccgggt cgtagccggc cgaggcggcg aggcgctgcc
    12181 cctccttgtc cgactcgtcc tcgtgctcgc ggctgtggct gcgcaggagc agctcgctga
    12241 ggatcaggct ggccgccgcc gccccctgta tcaggtcgcc tccagggtcg cgccccaccc
    12301 gcgggtcgta gggggcctcg tcacgcgtcc ggttgttgct gttcgcctcg cccaccagga
    12361 tgccggcgac caggaggttg ccgagcacgc tcatcagcgt cgccttgcgc ttcatgcgca
    12421 ggtagtgctc cagggtgacg tgggcgatct cgtgccccag gacgttggcc agcatgtcgt
    12481 cgtccagccc caggtcgagc atgccgcggg tgacgaagat ctggccgccc ggcagcgcga
    12541 aggcgttggg caccggcacg tccaccagcc cgaaggtgaa ggggaacttc tggaactcgg
    12601 aatgctgcgc cagctcgtag ccgatgcggt tgacccgcgc cagctcctcg gagttgtcgt
    12661 agagcccgta ctgctccacc gcctcctggg ccacggtcag gctcttggcg aagaggtccg
    12721 ggttggcgat ggtctgcgcc gccgccggcc gccaggggga ggccaggagc gccagcgcca
    12781 gggcgaaggc tacggagcgt cgggtcctgt cgcgcatggt tccagtgtac tccaggggta
    12841 gagttccaac cggtgacaac acggcgccgc gggggaaaac tcccggtcag cggggcagcg
    12901 cggcgtcctc cagctccttc tcggcggcct ccggcagcgg cgagcggggc gagcggagct
    12961 ggctctggat cgattcgggc ggcaggatgc ccccggaaac caccatccgc accccctcct
    13021 ccacggtgat ggcgcactcg atgagctgcc ggggcggcaa cagcacgatg tagccgctga
    13081 gcgggttggg cgtccggggg atcagcaccg gcaccagctc ctcgtcggcg cgcgggtccg
    13141 ccatcggcca gcggctgggc gcgccggtga cgaagccgat gctgaacacg ccgagccgcg
    13201 ggtactccac cagcaccacc cgcttgaagg cctcggcctg gtcgtgggag accgcctcca
    13261 gcaccccttt ggtggcgccg tagaccgact tgaccaccgg cacgcgcagg aagaacgcct
    13321 cgaccgagtc gaggaggcgc cggccgacca ggttggtgga gagccagccg aggaacagga
    13381 ccacggccag ggtgaggagg atgcccagcc ccgggatgtg gatggcgctt tcggggaaga
    13441 cctggcggag cgtccggtcg atgagcggcg cgaagatgcc gtccatgaag aggaacagca
    13501 cccgcagcac atagatggtg aggaagaccg gcgcgacgaa aaggagcccg gccaggagga
    13561 cggtgcggaa ccggtgccgc cagctcgggg aatgcgggtg ggccgggggc tcgagccctg
    13621 gaactgcgct caccccgata gcttagcatc gatggattct tcgggccggg gccgccctgt
    13681 ggtagcgttc cggcgcatga gcgatctgac catcgaggtg gagacggcca agtcgtccat
    13741 ggaagagatc cgcccgacgc tggacgccgg gctcaaggag gagttccccg gcggcatgct
    13801 gaagtggcgc tgggaggggg acgtgatgca tctctccggc cccggcgcct ccgccaccat
    13861 cgtctacgag cccggcaagc tcgtcggccg cgcccatctc aagccgccgg cgagcctgat
    13921 gaagcccgtc atccagcaga agatctcctc cgtgatgaag aaggccgccg tctagcggcg
    13981 aggccgtcgc cggtggcgat cctccccggc gccctgctgc gccacgccgc cgaacgcccc
    14041 gaggtcccgt ggctcttctg ggccgagggg tgggactggc gctggctctc ctggggcgag
    14101 gccgcgcggc gtgtcatggc cgaagcggac accttcgccg gcggcccacc tctcgtcccg
    14161 tccccggacc ccctcacccc tgccgccctg atcaccgacc tggccagcca ggccgccgga
    14221 gccgtggtga gagtgcgggg gagtggggac gagtggcagg agtggggggc cgaggatctg
    14281 ggcgccgcgg cggacgcggt cgcccgcgag gtggcgccgg cgggtccccc ggccggcggg
    14341 cgcgagatcc tggtcgccgg ccgtcccctc gccgagccgg cggagcgcgc cgtcgtcgcc
    14401 tgggcgatcc tccagggcgc cgccctgctc ctgga
//
LOCUS       DASPJI010000777         6191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_397863, whole genome shotgun sequence.
ACCESSION   DASPJI010000777 DASPJI010000000
VERSION     DASPJI010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397863"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VEL74_21775"
     CDS             complement(<1..280)
                     /locus_tag="VEL74_21775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65225.1"
                     /translation="MNRRSGAAPVGALALLLLTGAAPTPPPPPPAVGVTVTPISGSAG
                     ARRTDAVLPGRLLDTALPRGADGRRRLLALTAPDDPKASSAPEGPRALY"
     gene            complement(277..1989)
                     /locus_tag="VEL74_21780"
     CDS             complement(277..1989)
                     /locus_tag="VEL74_21780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HYG65226.1"
                     /translation="MTRPYLRPLLASAALLAVLLSPTHPASAAREKLALRYLPLPLPG
                     APSAVVAADIDGDGHRDLAVVVAYTGWGQIEIQESTTMDDIEGFVEVLTVIPSLVERR
                     EIRVFPGRPDGGFGPGAVALPMDTSVLTVEAGPPGLPVVAMTDDGLSVLRMKPGATVP
                     DVPELSWEKVITERSVLAGTGTFLSNLGLVRDVDGDQRADVLFPTLEGVSVYLSGAEG
                     LRTSQPARVRFPLDDLQRAGTSALVWRYPLPELRDVDGDRRPDMILFHPSGGLRGFRV
                     LRNLGDGRFGEPLAPLGDYQERASGRRREGRDGGAGTPVPVWFGDLDGDHKAEYVTQE
                     EKEIGEDAGMRAEMRAAKRPKFTYRLHRSRADLGMEPTPYQQFEVEGYALDGGSGEGE
                     LRFPGGLQDLNGDGKMDLTAITLDFSMLQAVKVMTVQRIGIGLDFHVWCQAAGGFRPV
                     RGLDLSGKFNLNLNNLKIGHLSLFEGDFDGDGRPEFVQMGRGRAVTIHRGRPDCGYNE
                     RPDLTLQLEEEPRDLSLAQIRELDGDGLSDLMVIQPQPPRRTDDKGVTQPVRLDLYLS
                     GGGR"
     gene            complement(2046..4442)
                     /locus_tag="VEL74_21785"
     CDS             complement(2046..4442)
                     /locus_tag="VEL74_21785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65227.1"
                     /translation="MTTWTPADLLPGLWVAALAALLALGLRRWFDPAPRWCWMVWGGV
                     LLVLFGPALFGGRVLLPLGYLTLLPPYRGAIWEGAPPGNLLASDVVLQITPWLVRVRE
                     AYAAGEWPLWNHLAGAGEPLLGNPQSQAFQPLAWLALPFPVPEAAGVIAALRVLVPLV
                     FTFLLLRRQGISEAPALWGSLAFGLSGFLHLWLGWPLASSATFLPVLLYGLAIVARRG
                     LGRDQALLAAATACVVLVGHPETGLHVVVLGTAFALSLLRETPAERRGALLRSWVIAG
                     WVGLGLAAPALMTAADALPRSLRASLLAARHAAPAAAAADPAPAVGRLLPVAAPNAYG
                     NNRFGHYWGNSNMNEDAAGFTGTAALLAFLLAFLPYRKEERLPQERVLLGAALVCLVV
                     LARPPGLVQLLEALPVLRESYSYHSRLTMLLNFVVAYLAACTWERWRRNGLRPRPVLL
                     AAAALAALILWGYLAHPHPEDPDRLALLRYGTLALQLAVLAVFTVALARPSRARWAPG
                     LLALAVAGELLAIYAPVNPSLPARLYYPETEPLTFVRQRLDPWYRMAGVGGALRPNLP
                     SVYGLADPRSSNPAKPAAYAEVTRRINRFPDRASDGFLHPEDPLYGLLGVRFVMTAPR
                     LQLPPPLKPVLRRPAGWVYRRPGALPRLFLPAAAAECPEPRPWPDCTAAVRDFGETAL
                     VRRLRGGTWKAAAPRDSILDLGDLRPAHLTVRARLAEPRLLASSLYQDDGWKLLAEGR
                     PLPTATANGPFLAAWLPAGDTRLDLLYRPSGFLLGMAMAALALAGAAILWLPPPRRVP
                     "
     gene            4726..>6191
                     /locus_tag="VEL74_21790"
     CDS             4726..>6191
                     /locus_tag="VEL74_21790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HYG65228.1"
                     /translation="MQRISDPRPSPRGDRVAYVLRTNDLEENRARTDLWMVNIAGGAG
                     GDAVRLTSDPAADNTPRWSPDGKSLYFLSTRSGSSQVWRLDMSAAKPGDPVQVTDLPL
                     EVANLLVSPDGSRLAFSLEVFPDCQTLACTRERLDHKEKGKVKAQLFEGGFVRHWDTW
                     SDGRRNHLFTASLDKDGKVGEPRDLVDLTRGMQGDAPSRPFGGTEEVAFSPDGRTVIF
                     TLRMAGEDNREEPWSTNFDLWEVPADGSRAPRNLTAANPAWDTQPAFSPDGKTLAYLA
                     MSRPGFEADRFRIVLRNLADGKERVLTEAWDRSPGEIVFSADGRTIYSTSLDIGQLPL
                     FAIDVASGQVRKVVAEGSAHSPALAGDRIVFGLDTHHSPVELYTVRPDGSGLTPITHV
                     NRERLADVLMGQAEQFSFQGAKGDRVYAWVVKPAGFQEGKKYPLAFLIHGGPQGSFHN
                     EFHYRWNPQVYAGAGYASLLIDFHGSLGYGQAFTDAIRG"
BASE COUNT          942 a         2160 c         2307 g          782 t
ORIGIN      
        1 gataaagagc gcgggggccc tccggggcgc tcgaggcttt cgggtcgtca ggagccgtca
       61 gggctaggag ccggcggcgg ccatcggcgc cccgcggcag ggccgtgtcc agcaggcggc
      121 ccggcaggac cgcgtccgtt cgccgggctc cggcgctccc ggagatcggg gtgaccgtca
      181 ctccgaccgc cggaggtggg ggcggcggcg tgggagcggc gcccgtcagc aggaggaggg
      241 cgagggcgcc cacaggggcc gcaccgctgc ggcgattcat cggccacctc cgctcagata
      301 gagatccaga cgcaccggct gggtgacgcc cttgtcatcg gttcttcgtg gcggctgggg
      361 ttggatcacc atcagatccg agagcccatc gccgtccagc tcgcggatct gcgccaggct
      421 cagatcgcgc ggctcctcct cgagctgcag ggtgaggtcg ggtctctcgt tgtagccgca
      481 gtccggccgg ccgcggtgga tggtcacggc gcggccccgg cccatctgca cgaactcggg
      541 gcgcccgtcg ccgtcgaaat ccccttcgaa cagcgagaga tggccgattt tcaggttgtt
      601 caggttcagg ttgaacttgc ccgacaggtc gagcccgcgc accggccgga agccgccggc
      661 cgcctggcac cagacgtgga agtcgagccc gatgccgatc cgctggacgg tcatgacctt
      721 gaccgcctgc agcatggaga agtcgagggt gatggcggtc aggtccatct tgccgtcgcc
      781 gttcaggtcc tggaggccgc cggggaaccg aagctccccc tcgccgctgc cgccgtccag
      841 ggcatatccc tccacctcga actgctgata gggcgtgggc tccatgccca ggtcggcgcg
      901 tgaccgatgg agacggtagg tgaatttcgg gcgtttggcg gcccgcatct ccgcccgcat
      961 ccccgcgtct tcgccgatct ccttctcctc ctgggtgacg tactcggcct tgtgatcgcc
     1021 gtcgaggtcg ccgaaccaga ccggcacggg ggtaccggcg cctccgtctc gtccttcgcg
     1081 ccgccgaccg gacgcgcgct cctggtaatc ccccaggggg gcgagcggct cgccgaaacg
     1141 gccgtcaccc aggttgcgga gcacgcggaa gccgcggagc ccgccgctgg gatggaagag
     1201 gatcatgtcg ggccggcggt cgccgtcgac gtcgcgcagt tcggggagcg gatagcgcca
     1261 gaccagggcc gaggtgccgg cgcgctggag gtcgtccagc gggaaacgga cccgcgcggg
     1321 ctgcgaggtc cgcagcccct cggcgccgct cagatagacc gacacgccct cgagggtcgg
     1381 aaagaggacg tccgcccgct ggtcgccgtc gacgtcgcgc accaggccca ggttggagag
     1441 gaacgtgccg gtgccggcga ggacggagcg ctccgtgatc accttctccc acgacagctc
     1501 ggggacgtcg gggacggtgg cgccgggctt catgcgcagc acggacagcc cgtcgtcggt
     1561 catggccacg accggcagcc cgggcgggcc ggcctcaacg gtgaggaccg acgtatccat
     1621 ggggagcgcc accgcgccgg gaccgaaacc gccgtccgga cgcccgggga agacgcggat
     1681 ctcgcggcgc tccaccaggg aggggatgac ggtgaggacc tccacgaacc cctcgatgtc
     1741 gtccatcgtg gtcgattcct ggatctcgat ctgaccccac ccggtgtagg ccaccaccac
     1801 ggccaggtcc cggtgcccgt cgccgtcgat gtccgcggcg acgacggccg acggcgcgcc
     1861 cggcagcggc aggggcagat agcgaagcgc cagcttctcc cgtgccgccg aggcggggtg
     1921 ggtgggggag aggaggacgg cgaggagagc ggcggaggcg aggagaggac gaaggtacgg
     1981 tcgggtcata gacgtgcgat tcctctcagg agtacgtcgg cggcggcgaa atgttacccg
     2041 acctctcagg gaacgcgccg cggcggcggc agccagagga tggccgcgcc ggccagggcc
     2101 agcgcggcca tcgccatccc caggaggaac cccgacggtc tgtagaggag gtccagccgg
     2161 gtatcccccg ccggcagcca ggcggccagg aaggggccgt tggcggtggc cgtcggcagt
     2221 ggtcttccct cggcgagcag cttccagcca tcatcctgat agaggctgga ggccaggagg
     2281 cggggctcgg ctaggcgtgc ccggacggtg aggtgggccg gccgcagatc cccgaggtcg
     2341 aggatggaat cccggggggc ggcggccttc caggttcctc cacgtagccg gcggaccaag
     2401 gccgtctcgc cgaagtcgcg gacggcggcg gtgcagtccg gccatgggcg cggctccggg
     2461 cactccgcgg ccgccgcggg gaggaagagc cgggggagcg ccccaggtct ccggtagacc
     2521 cagccggccg gacgccggag cacgggtttc aggggcggcg gcaactggag gcgcggcgcg
     2581 gtcatcacga agcggacgcc caggaggccg tagagcgggt cctccgggtg gaggaagccg
     2641 tccgaggcgc ggtccgggaa gcggttgatc cgccgggtga cctctgcata ggcggcgggt
     2701 ttggccggat tcgagctgcg cgggtccgcc aggccgtaga ccgaggggag gttcgggcgg
     2761 agggcgccac ccacgccggc catccggtac caggggtcca gccgctgccg tacgaaggtg
     2821 aggggctcgg tctccggata gtagaggcgg gccggcagcg acggattgac cggcgcgtag
     2881 atggccagca gctcgccggc gacggccagc gccaggagcc cgggggccca gcgggcgcgg
     2941 gagggccggg cgagggccac cgtgaacacc gcgagcaccg cgagctggag ggcgagcgtc
     3001 ccgtaacgga ggagcgcgag ccggtccgga tcctcggggt gcggatgcgc caggtagccc
     3061 cagaggatca gggccgccag cgccgctgcg gcgaggagca ccggccgggg acggagcccg
     3121 ttcctcctcc agcgctccca ggtgcaggcg gccaggtagg cgacgacgaa gttcagcagc
     3181 atcgtgaggc ggctgtggta gctataggac tcccgcagca ccggcagcgc ctcgaggagc
     3241 tggaccagcc ccggcggccg ggccaggacc accaggcaga ccagggccgc acccaggagg
     3301 accctctcct gcggcagccg ctcctccttt ctataaggaa ggaaggcgag caggaaggcg
     3361 aggagggccg ccgtgccggt gaagccggcg gcgtcctcgt tcatgttgct gttcccccag
     3421 tagtggccga accggttgtt gccgtaggcg ttgggggcgg ccaccggaag gaggcggccc
     3481 accgccgggg ccgggtcggc cgcggcagcg gccggggcgg cgtgccgggc ggcgaggagc
     3541 gaggcgcgga ggctgcgggg gagggcgtcc gcggccgtca tcagggctgg agcggcgagc
     3601 cccagcccca cccagccggc gatcacccag gagcggagca gggcaccccg tcgctcggcc
     3661 ggcgtctctc ggaggaggga gagggcgaag gccgttccga ggaccaccac gtgcagcccg
     3721 gtctccgggt gcccgacgag gacgacgcag gccgtggccg ccgccaggag cgcctggtcc
     3781 cgtcccaggc cgcgccgggc cacgatcgcc agaccataga gaaggacggg gagaaaggtg
     3841 gcggagctgg ccagcggcca gccgagccag aggtggagga accccgagag gccgaaggcg
     3901 aggctccccc agagcgccgg cgcctccgag atcccctgcc ggcgcagcaa gagaaaggtg
     3961 aacaccaggg ggaccagcac ccgcagcgcg gcgatcaccc cggcggcttc gggaaccggg
     4021 aaggggaggg cgagccaggc caggggctgg aacgcctgac tttgggggtt gcccagcagc
     4081 ggctcgccgg ccccggcgag gtggttccac agcggccact cgcccgccgc gtacgcctcc
     4141 cggacccgca ccagccaggg ggtgatctgg aggacgacgt cgctcgccag caggttgccc
     4201 ggcggagccc cctcccagat ggctccccgg taggggggga gcagggtgag gtagcccagc
     4261 ggcagcagca cccggccccc gaagagcgcc ggcccgaaca ggacgaggag cacgccgccc
     4321 cagaccatcc agcaccagcg gggggccggg tcgaaccagc gccgcaggcc gagggccagg
     4381 agcgccgcga gggcggcgac ccagagtccg ggcaggaggt cggcgggcgt ccaggtggtc
     4441 atgcgatgga gtccggatga gaacccgaaa cctcgaccga tgcaaccgcc gccccgccgg
     4501 cggctcgatc ttcctccatc cccggctcgg tgccctaaat ccggcgcccc ggccacctcg
     4561 gcccgcgctt cggaccgcga aatgggtcaa aagctgcccc atgggctatg atctctgccc
     4621 atgcgccccg ttcagagcct gttttcgcct cttattctct tctgtcttgc agcggctcct
     4681 gccgccgcgg ctccgcaccc gttcacggcc cgtgacctcc agtccatgca gcgcatctcc
     4741 gatccgcggc cgtcgccgcg cggggaccgg gtggcctacg tcctgcgcac caacgacctc
     4801 gaggagaacc gggcgcggac cgacctctgg atggtgaata tcgccggggg tgccggcggc
     4861 gacgccgtgc gcctgacctc cgatccggcg gcggacaaca ccccgcgctg gtcgccggac
     4921 ggcaagagcc tctacttcct gtccacccgc tcgggctcaa gccaggtctg gcggctcgac
     4981 atgtccgcgg cgaagccggg cgaccccgtg caggtgaccg acctgccgct cgaggtcgcc
     5041 aacctcctgg tctcgcccga cggctcgcgc ctcgccttca gcctggaggt cttccccgac
     5101 tgccagaccc tggcctgcac ccgcgagcgc ctcgaccaca aggagaaggg caaggtcaag
     5161 gcgcagctct tcgagggcgg cttcgtccgg cactgggaca cctggagcga cggccggcgc
     5221 aatcacctgt tcacggcctc cctggacaag gacggcaagg tgggcgagcc gcgcgatctc
     5281 gttgatctca ccaggggcat gcagggggac gcgccgtcgc ggccgttcgg cggcaccgag
     5341 gaggtcgcct tctcgcccga cggccggacc gtgatcttca ccctgcgcat ggcgggggag
     5401 gacaaccggg aggagccctg gtccaccaac ttcgacctct gggaggtgcc ggcggacggc
     5461 tcccgggcgc cgcgcaacct gaccgcggcc aacccggcgt gggacaccca gccggccttc
     5521 tccccggacg gcaagaccct ggcctacctc gccatgtcgc gccccggctt cgaggcggac
     5581 cgcttccgca tcgtgctgcg caacctggcg gatgggaaag agcgggtgct caccgaggcc
     5641 tgggaccgct cgcccggcga gatcgtgttc tcggcggacg gccgcacgat ctactccacc
     5701 agcctggaca tcggccagct tcctcttttc gccatcgacg tcgcctccgg acaggtgcgc
     5761 aaggtggtgg cggaggggag cgcccactcc ccggcgctgg cgggcgaccg catcgtcttc
     5821 ggcctcgaca cccaccactc gccggtcgag ctgtacaccg tgcggccgga cggctcgggc
     5881 ctgactccga tcacccacgt caaccgcgag cggctcgcgg acgtcctcat ggggcaggcc
     5941 gagcagttct ccttccaggg ggccaagggg gaccgggtgt atgcctgggt ggtcaagccc
     6001 gccgggttcc aggagggcaa gaagtacccg ctggccttcc tgatccacgg cggaccccag
     6061 gggtcgttcc acaacgagtt ccactaccgg tggaaccccc aggtctacgc cggcgccggc
     6121 tatgcctcgc tgctcatcga cttccacggc tccctgggct acggccaggc gttcaccgac
     6181 gccatccgcg g
//
LOCUS       DASPJI010000778        12019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_853270, whole genome shotgun sequence.
ACCESSION   DASPJI010000778 DASPJI010000000
VERSION     DASPJI010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12019)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12019
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853270"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="VEL74_21795"
     CDS             complement(<1..1358)
                     /locus_tag="VEL74_21795"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYG65229.1"
                     /translation="MHPKETATLPTRACRPTVLVLLALFVLPFLAASLSAQPPAADRK
                     MIVLGFDGADAKLAQQWMDEGKLPNLAKLRQQGTFAPLRSTIPSQTPVSWSTFSTGLS
                     PGRHGIFDFLKRDPATYRPSFAAADVSRAPFLFGERNPLVLGAIATVVLALLLLLLLK
                     LFRMRTKVAAAVAVALGLVAGVGTGYAASTLLPTSRPVAINNQQGDTFWALLGKAGKR
                     VEVMRVPVTFPPKAYEHGHLLTGLGTPDLSARIGKPFYFTSELFFQPKGGGDFSVEVT
                     ELVDNRGVIDTEIKGPPNELFPPAEGEEMQYISIPMRLTVAEDRSSLGIEVSGTQLSL
                     KPGEWSDWVAFSFPFNRLIKVGGIGKFRLVSLDPEVRLYLSPIQFDPKGMPPGLEITT
                     PADFIDQLASRFGRFKTIGWMIDTWSLPNGTIDEQVFLEDVRQTVDKEKEILRGLLAD
                     DD"
     gene            1483..3804
                     /locus_tag="VEL74_21800"
     CDS             1483..3804
                     /locus_tag="VEL74_21800"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HYG65230.1"
                     /translation="MESAAENRRPYGMLLLGALVLAAGVLLARQWLPEWQTWRLPHEA
                     AFEERHRETAGRLGVRLSPDAPRTRLVVSDDTVGDVRWTAGDASVAELEALGAGLKIE
                     VVREGTLAGDAPARQLGIDFSPSGRPLQLAWMKRVPSLVFEGAKEPASPEVVERVAAG
                     VLAPGETLADPSEKRGASGLEMRYDLPGTRPPQHLLAQPLPGGNIVVTRGAGSVEEAA
                     RRGEGQSVAGELLKALPRFLAFLVVVVLFFALLFKRRIDFVNGLFLGAISFVASAATL
                     FSHPDPLALLEIAVPALFVALWVVLIWSTGESLLRSTDSSFTTSLDTLRAGRLGPRGG
                     RALLYGLSLGAALGGLRLAVLAGAAALPGLAPGASSVPLPAFAADHSPFSTGILIAAE
                     VMLIVACARRLLAPRWAVAAAIIVGALLVEPVPLQPFAAAFVANLVTVGLLVWLGRRF
                     GLTALLTASIAVYLLPTVVLAASALHWLPVTFAVTAGTTAGVLVLGFVGLRRPGRVEL
                     ERLAPPRFMRRLEEERRIKYEMDLLARMQLGLLPADVPAVPGWDIAARSLLATEAGGD
                     LYDFLLDDAGHLWIAAGDVAGHGYSCSIVQAMTTAALTSLIGPGKSPAEVLLGVDRVI
                     RRGGSHRNFTSLTLLRLDPGTGEAVLANAGHPYPLALAEGTVEELEIPGLPLGQGPRR
                     QYRDVDLQIPPGGALVLCSDGLFEAVNWESAPYGFDRPREVLRTLEERPAAEILETLL
                     ADWKRHVSAEAPPDDTTVVVIKRAAFDRSVTVT"
     gene            3896..4828
                     /locus_tag="VEL74_21805"
     CDS             3896..4828
                     /locus_tag="VEL74_21805"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HYG65231.1"
                     /translation="MKLTPLGKALVFLIGLGLFITALYKFVPPEQQIWRKWLGGGGDT
                     AATVENQGTEPAAEGERGPDTLANQPIDRNGESRGEEPRQERRAASEWITVPGGLFRS
                     GEGLEEVDVPAFQIQSHEVTNGQYEAFLEECPEGSSCGPRDLPSYWDDQSYLETRRDA
                     PVVFVSWADASAYCRWAGGRLPTISEWEKAARSDDGRNFPAGPSIDPADVNILGQDRR
                     DEKNRAPKQIPTWGTDDPRYNRDASPYGVLAMAGNVSEWTATASPDEPDLRLAAGGSW
                     DSWSLDDARTYASLPKQPADRSSSLGFRCVKSAR"
     gene            4829..5815
                     /locus_tag="VEL74_21810"
     CDS             4829..5815
                     /locus_tag="VEL74_21810"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HYG65232.1"
                     /translation="MNNDYPEVTTMMIRRISITAILLVLGISTFATAQGDESLQRVVD
                     SGTLRVAAEPGSPPMLVKEGNRYDGFDYAIAKALAKRIGVDSVVIVAGKYSELPGRLI
                     AGKADVIISGYTADPSIAGVDWSEPYLNYGLALVVRKGSLIRSIDDLRGKVVGIFNDP
                     AAEADVKRLVKGYKDLRKFEDGYFDLLADGQLDAFIYDFPFAQEEIKEYDGKLEIVQF
                     NLTDSTYNVGVRKGATSLLRMINSEIRSLKGSEEYRQIIRKYLGGTGPAPVTKVAADQ
                     TVYKVQRGDTLSGIAQKRLGRMSRWEEIWDLNKGRIADPNLIEIGWELVLPK"
     gene            complement(5871..6284)
                     /locus_tag="VEL74_21815"
     CDS             complement(5871..6284)
                     /locus_tag="VEL74_21815"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYG65233.1"
                     /translation="MILVDTGPFVALFDPRDGRHQRCRAVLRDLREPLVTTVPVLTEA
                     FHLLEPSSIGSSRLRDFVLRGGVSVWFMDAPALTRSFELMQQYTDHPMDLADASLIAA
                     AEALPTRRVFTIDRGDFSAYRVRRGKRFEPVEIVG"
     gene            complement(6281..6541)
                     /locus_tag="VEL74_21820"
     CDS             complement(6281..6541)
                     /locus_tag="VEL74_21820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65234.1"
                     /translation="MALRTVRLDDDTEEVLEDLVESTGLSISAVFKQGLMALRDRLSA
                     QEGRSAYEIYEALDLGPGGYAVAPSTETRRGVREAIRRKHDR"
     gene            complement(6578..7093)
                     /locus_tag="VEL74_21825"
     CDS             complement(6578..7093)
                     /locus_tag="VEL74_21825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HYG65235.1"
                     /translation="MPTIAELLLPELEREAAGTRRALERVPEGRDEWKPHEKSMPLGY
                     LAGLVAIMPSWVDSIVRQDELELSAPGEYGAGAPPTNRELLEMFDKAVAKAKDALASA
                     TDEHLTGSSWRLLLQGKVVYEETRYVAIRDSVFNHLAHHRGQLTVYLRLNEAPVPSLY
                     GPSADEKWDGG"
     gene            complement(7131..8888)
                     /locus_tag="VEL74_21830"
     CDS             complement(7131..8888)
                     /locus_tag="VEL74_21830"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYG65236.1"
                     /translation="MSTVATPPAAFVESLPPWGAELVRAVWAKQANTFVLHGVPADLV
                     PVRGSSGLRFLSLDEFVTKELFAGWQSIVTYNRAEGLGFSTPAARGHFQERLRNYDSV
                     HGTNWAEALPRDAANCFALLDSYFRHCSSLQPSRPVVLLLPFAETVIPAGDASYRTPE
                     DRAVLVYLRKWSQDPVLLAKNMVIVLITESLAELDPKLLRAHATREVEIVRPDPRERL
                     EYLEAVRPADWYKRRSELPPVRLAELTAGMTRVQLGQMLDGADAHDETLTREQVRRVK
                     KEVIETEGLGLLEYVEPKYDLSMVAGMPAVKDRLRRAARAIARGNPAAVPMGYLVCGP
                     VGSAKTFLVECFANEIGFPCVKLKNFRSMWVGATESNLERILKILSSLTPVGVVVDEA
                     DAALGNREQSGDSGVQTRVFAQIASFMADTRHRGKILWFLITARPDLLPIDLKRQGRA
                     EEHIPLFYPETVEEYDEIYQVMVKKLSLKTPVTELSKVVEEEQLLGLSGSDLEAVLVR
                     AVLEAEAAEASEVAEEHIKKAFGDFIPPANSLEREMQILFAVLESTSRELLPEKFRDL
                     DRAAIQARAQEIKRALRLG"
     gene            complement(8898..9761)
                     /locus_tag="VEL74_21835"
     CDS             complement(8898..9761)
                     /locus_tag="VEL74_21835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65237.1"
                     /translation="MGFLDRHPVLKKAVANQYQAILVTGAVAFSALTASPLPLLALAG
                     LELMAAPFMIERIKRRLDIEKKYADRQFQTLSQEQRFEALTPSAKARFVRLRQLCERI
                     QNNYKGLSAASQGMLADQDNKFDSILATCLRHLWLIQKYDEMVGSFNEPQARADIGKL
                     KEQLAAPDVDERVREALEKHLEIKEQLLRTVRQNVANRQALLAEVDSLESLLQLLLQK
                     SIAATDATAFSLEIDDVLARAEADAASVEEMERLFGAMPETGEPILSGKIKQMTAPPP
                     PPPPARGKQRR"
     gene            complement(9783..10550)
                     /locus_tag="VEL74_21840"
     CDS             complement(9783..10550)
                     /locus_tag="VEL74_21840"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HYG65238.1"
                     /translation="MFKRLARVFKSWVGYFISFAEDPEVMLQESIEEMRNTLPRLNQI
                     LVTTRATVIRLEQERDELVRKEKQLTSSIQAALKDGSPEARRLAEEDAVHLQTVRQDL
                     QATSEQLMAAQNAFQNAQISVEDIKGKLRARMDQARKAIQESKKAEVMRSAANALAEL
                     DTYGTASTTDKYLEEIKQRVAESKAAVEVATGGLDVDRIKMERKARSIQAQSILSEFE
                     VEMGLKQPDATPEAAPFGTATATPTAEATPQRAREGG"
     gene            complement(10571..11374)
                     /locus_tag="VEL74_21845"
     CDS             complement(10571..11374)
                     /locus_tag="VEL74_21845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65239.1"
                     /translation="MKIVELDSPAPAVTAASPAAGASHPAAGSGAMPAARPGKPTDVR
                     EILGSIPEARIDEQALPRSGATAGAPAAAGTAGLEIPDFEAIYKAAGISEPPHGYTAF
                     KVLEILESDHFAGLDHKARAAALAGFLKMNPTGPVPIADVIQDAVRRDQALDKFEELL
                     RGKLAARAQSVEQENARLQAEIDELVRRHQETMEANRKAVEAEQARLGQWQVRKRIEE
                     RRLYESVGPFVEENPVSAEGVPGSTVRTPAPASPPAGTPASEGATGSDR"
     gene            complement(11585..11815)
                     /locus_tag="VEL74_21850"
     CDS             complement(11585..11815)
                     /locus_tag="VEL74_21850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65240.1"
                     /translation="MSAARRTIGRWILAALLERGCDPTTARVPRRVFSIRPLTSREMA
                     ELNPLSSSLVPLSNLRRMRLPRNPNARPTYEV"
BASE COUNT         1773 a         4232 c         4126 g         1888 t
ORIGIN      
        1 cagtcgtcgt cggccaggag gccgcggagg atctccttct ccttgtcgac ggtctgccgg
       61 acgtcctcca ggaacacctg ctcgtcgatg gtgccgttgg gcagcgacca ggtgtcgatc
      121 atccagccga tggtcttgaa acggccgaag cggctggcga gctggtcgat gaagtccgcc
      181 ggcgtggtga tctccaggcc cggcggcatc cccttggggt cgaactggat cggcgacagg
      241 tacagccgca cctcggggtc gagggagacc agccggaact tgccgatgcc tccgaccttg
      301 atcagccggt tgaacgggaa gctgaacgcc acccagtcgc tccactcgcc cggcttcagg
      361 ctcagctggg tgcccgacac ctcgatgccc aggctggaac ggtcctcggc cacggtcagc
      421 cgcatgggga tggagatgta ctgcatctcc tccccctcgg ccggagggaa caactcgttg
      481 ggcggcccct tgatctccgt atcgatgaca ccgcggttgt ccaccagctc cgtcacctcg
      541 accgagaagt cgccgccccc cttgggctgg aaaaacagct cggaggtgaa gtagaacggc
      601 ttgccgatcc gcgccgagag gtccggcgtg cccaggccgg tgagcaggtg gccatgctca
      661 taggccttgg gcgggaaggt caccggcacc cgcatgacct ccacccgctt gcccgccttg
      721 ccgagcaacg cccagaaggt gtccccctgc tggttgttga tggcgacagg ccggctggtg
      781 ggcagtaggg tgctcgccgc gtagccggtc ccaacacctg ccacgagccc gagggccacc
      841 gccaccgccg ccgccacctt ggtgcgcatc cggaagagct tcagcagcag gagcagcagc
      901 agggcgagga ccacggtggc aatggcgccc aggaccagcg gattcctctc gccgaagagg
      961 aagggcgcgc gcgagacgtc cgccgccgcg aagctcggcc ggtaggtggc cgggtcgcgc
     1021 ttcagaaagt cgaagatgcc gtgccggccc gggctcagac ccgtggagaa cgtcgaccag
     1081 gagaccggcg tctgcgacgg gatggtggag cgcagagggg cgaacgtccc ctgctgccgg
     1141 agcttggcca ggttcggcag cttcccctcg tccatccact gctgggcgag cttggcgtcg
     1201 gcgccgtcga agcccaggac gatcatcttg cggtcggccg ccggcggctg ggccgagagg
     1261 ctggcggcca ggaacggcag gacgaaaagc gcgagcagga ccagaacggt gggccggcac
     1321 gcgcgcgtgg gcagagttgc agtttctttt ggatgcatag aggtcgcggc ttccgaaggg
     1381 tcgatcggtg tgagccgcgg attctatcaa accgaaaccc tcggagcggc gccgcgtatg
     1441 agaaggagca cccggggtct gagagaatga ccccaccagc ccatggaatc cgccgccgaa
     1501 aaccgccgcc cgtacggcat gctcctcctg ggtgccctgg tgctcgccgc cggggtcctg
     1561 ctcgcccggc agtggctgcc ggagtggcag acctggcggc tgccgcacga ggccgcgttc
     1621 gaagagcgcc accgcgagac cgccggccgt ctcggggtcc ggctctcccc ggacgccccg
     1681 cgcacccggc tggtggtgag cgacgatacg gttggcgacg tccgctggac cgcgggcgac
     1741 gccagcgtgg cggagctcga ggccctcggc gccgggctca agatcgaggt ggtccgcgaa
     1801 gggaccctcg cgggcgacgc gccggcacgc cagttaggga tcgacttctc tccctcgggc
     1861 cggccgctcc agttggcatg gatgaagcgc gtcccctccc tcgtcttcga gggggccaag
     1921 gagcccgcct cccccgaggt ggtggaacgc gtcgccgccg gggtgctggc acccggcgag
     1981 acgctggccg acccgtccga gaagcgcggc gcctccggcc tggagatgcg ctacgacctg
     2041 ccggggacac ggccgcccca gcacctgctc gcccagcccc tgcccggcgg caacatcgtc
     2101 gtgacgcgcg gcgccggcag cgtcgaggag gccgcgcggc gtggcgaggg gcaatcggtg
     2161 gccggggagc tcctcaaggc cctcccgcgc ttcctcgcct tcctcgttgt ggtggtgctc
     2221 ttttttgccc tgctcttcaa gcggcgcatc gacttcgtca acggcctctt cctgggggcg
     2281 atctccttcg tcgcctcggc ggcaaccctg ttcagccatc cggacccgct ggccctgctg
     2341 gagatcgcgg tcccggccct cttcgtggcg ctctgggtgg tgctcatctg gtcgaccggc
     2401 gagtcgctcc tgcgttccac cgattcgagc ttcaccacca gcctcgacac cctgcgcgcc
     2461 ggccggctgg gtccccgcgg cggacgcgca ctgctctacg gcctgtccct cggcgcggcg
     2521 ctcggcggcc tgcgcctggc cgtgctggcc ggagccgcgg cccttcccgg cctcgctccc
     2581 ggcgcctcga gcgtgccgct gcctgccttc gccgccgacc atagtccgtt cagcaccggc
     2641 atcctcatcg ccgccgaggt gatgctcatc gtcgcctgcg cccggcggct gctggcgccc
     2701 cgctgggcgg tggcggcggc catcattgtg ggtgccctgc tggtggagcc ggtgccgctg
     2761 cagccgttcg ccgccgcctt cgtggcgaac ctggtgaccg tcgggctcct ggtgtggctc
     2821 ggccgccgtt tcggcctgac cgccctgctg accgcctcca tcgccgtcta cctgctgccg
     2881 acggtggtgc tcgcggcctc ggccctccac tggctgccgg tcaccttcgc cgtcaccgcc
     2941 ggcaccacgg ccggcgtcct cgtcctcggc ttcgtgggcc tgcgccggcc cggccgggtg
     3001 gagctggaac ggctggcgcc gccccgcttc atgcgccgcc tggaagagga gcggcggatc
     3061 aagtacgaga tggacctcct cgcccgcatg cagctcggcc tgctgccggc ggacgtcccg
     3121 gcggtgccgg gctgggacat cgccgcccgg tcgctcctcg ccaccgaggc cggaggggac
     3181 ctctatgact tcctgctgga cgacgccggc cacctctgga tcgccgccgg cgacgtcgcg
     3241 ggccacggct actcctgctc gatcgtccag gcgatgacca cggcggcctt gaccagcctc
     3301 atcggccccg gcaagagccc ggccgaggtg ctgctggggg tagaccgggt gatccgccgc
     3361 ggcggctcgc accgcaactt caccagcctc accctgctcc gtctcgaccc ggggaccggc
     3421 gaggcggtgc tggccaacgc cggccatccc tacccgttgg ccctggccga gggcaccgtg
     3481 gaggagctcg agatccccgg cctccccctg ggacagggac cccgccgcca gtaccgggac
     3541 gtcgatctcc agatcccccc cggaggggcg ctggtcctct gctccgacgg cctgttcgag
     3601 gcggtcaact gggagtcggc cccctacggc ttcgaccgcc cgcgggaggt gctgcgcacc
     3661 ctggaggagc ggccggccgc cgagatcctg gagacgctgc tggccgactg gaagcgtcac
     3721 gtgagtgccg aggcgccgcc ggacgacacc accgtggtgg tcatcaagag ggcggcgttt
     3781 gaccgctccg tcacggtcac atagcatcat cgattcgcgg catggccgcc aaggacccga
     3841 atccaatgtt cgagaacagg cgtccgctcc ccgggagcgg acaggaggca tcgcgatgaa
     3901 actgaccccg ttgggcaagg ccctcgtctt cctcatcggc ctcggccttt tcataacggc
     3961 tctctacaag ttcgtgccgc cggagcaaca gatctggcgc aaatggctgg gtggcggagg
     4021 ggatacggcc gctaccgtcg agaaccaggg caccgagccc gccgccgaag gggagcgggg
     4081 cccggacacc ctcgccaatc agccgatcga ccgcaacggc gagtcgcggg gcgaggagcc
     4141 ccgtcaggag cggcgggcgg cgagcgagtg gatcaccgtc cccggcgggc tcttccgttc
     4201 gggcgagggt ctcgaggagg tggacgtgcc cgccttccag atccagagcc acgaggtgac
     4261 caacggccag tacgaggcgt tcctggagga gtgccccgag ggatcgtcgt gcggcccgcg
     4321 cgacctgccg tcctactggg acgaccagtc ctaccttgag acccggcgcg acgcgccggt
     4381 ggtcttcgtc tcctgggccg acgcctcggc ctactgccgc tgggcgggcg ggcggctgcc
     4441 gaccatttcc gagtgggaga aggcggcgcg cagcgacgac ggccggaact tccccgcggg
     4501 cccgtccatc gacccggccg acgtcaacat cctgggccag gaccgccgcg acgagaagaa
     4561 ccgggcgccg aagcagatcc ccacctgggg aaccgacgac ccgcgctaca accgggacgc
     4621 cagcccctac ggcgtgctcg ccatggcggg caacgtcagc gagtggacgg cgaccgcgag
     4681 ccccgacgag cccgacctgc ggctcgccgc cggcggctcc tgggactcct ggagcctcga
     4741 cgatgcccgc acctatgcct ccttacccaa gcagccggcc gaccgcagct cgagcctcgg
     4801 cttccgctgc gtcaagagcg cccggtaaat gaacaacgac tatccggagg tcacgaccat
     4861 gatgatccga cgcatctcca tcacggcgat cctcctcgtt ctggggatct ccaccttcgc
     4921 caccgcccaa ggagacgaat cgctgcagcg ggtcgtcgac agcggcaccc tccgcgtcgc
     4981 ggccgagccc ggctcgcccc cgatgctggt caaggagggc aaccgctacg acggcttcga
     5041 ctacgccatc gccaaggcgc tcgccaagcg catcggcgtc gactcggtgg tcatcgtcgc
     5101 cggcaagtac agcgagctgc ccggccgcct gatcgccggc aaggccgacg tcatcatcag
     5161 cggctacacc gccgacccgt ccatcgccgg cgtcgactgg tccgagccct atctcaacta
     5221 cggcctcgcg ctggtggtgc gcaaggggag cctgatccgc agcatcgacg acctgcgcgg
     5281 caaggtggtg ggcatcttca acgaccccgc ggccgaggcg gacgtcaagc ggctggtcaa
     5341 gggctacaag gacctccgga agttcgagga cggctacttc gacctcctcg ccgacggtca
     5401 gctggacgcc ttcatctacg acttcccctt cgcccaggag gagatcaagg agtacgacgg
     5461 caagctggag atcgtgcagt tcaacctgac cgactccacc tacaacgtcg gcgtgcgcaa
     5521 gggggcaacc agcctcctgc ggatgatcaa ctccgagatc cggtcgctca aggggtcgga
     5581 ggagtaccgt cagatcatcc ggaagtacct gggcggcacc ggcccggcgc cggtgaccaa
     5641 ggtggccgcc gatcagacgg tctacaaggt ccagcggggg gacaccctct cggggatcgc
     5701 ccagaagcgc ctcggcagga tgtcgcgctg ggaagagatc tgggacctca acaagggccg
     5761 catcgccgac cccaacctga tcgagatcgg ttgggagctg gtactgccga agtagcttcg
     5821 ccggccgcgg gcgccggctc accggcgccc gcgtgccatc ccaccggccc tcagccgacg
     5881 atctctacag gttcgaacct cttccctctc cgcacccgat acgccgagaa gtctcctcga
     5941 tcgatggtga agacccggcg ggtcgggaga gcctccgccg ccgcgatgag cgaagcatcg
     6001 gccaagtcca tgggatggtc ggtgtactgc tgcatcagct cgaacgacct cgtgagcgcc
     6061 ggcgcgtcca tgaaccagac cgatacgccg ccccggagga cgaagtcgcg gagccggctg
     6121 gagccgatgc tggagggttc cagcagatgg aaagcctcgg tcagcaccgg cacggtcgtc
     6181 accagaggct cgcggagatc gcggaggacg gctcgacagc gctgatgtcg gccgtcacga
     6241 gggtcgaaga gagccacgaa cgggccggtg tcgaccagga tcaccgatcg tgctttctcc
     6301 ggatcgcctc ccggacgccc cgccgtgtct cggtggacgg cgcgaccgca tatccacccg
     6361 gcccgaggtc caacgcctcg tagatctcat aggccgaccg gccctcctgg gcggagagcc
     6421 ggtcccgcag cgccatgagc ccttgcttga acacggccga gatggacaag cccgtggact
     6481 cgaccaggtc ctccagaact tcttcggtat cgtcgtccag ccgcacggta cggagagcca
     6541 tgggtccccc tccttttgta atacgattgt attacagcta cccgccgtcc cacttctcat
     6601 ccgccgacgg tccatagagc gacgggaccg gagcctcgtt cagccgcagg tagaccgtca
     6661 actgcccccg gtggtgcgcc aggtgattga agacgctgtc gcggatcgcg acgtagcgcg
     6721 tctcctcgta gaccaccttc ccctggagga gcaggcgcca ggaggagccc gtcaggtgct
     6781 cgtccgtcgc cgaggcgagg gcatccttcg ctttggccac cgccttgtcg aacatctcca
     6841 ggagctcccg gttcgtgggc ggggctccgg ctccgtactc gcccggggcg ctgagctcca
     6901 gctcatcctg ccgcacgatc gagtccaccc aggagggcat gatcgcgacc aggccggcca
     6961 gatagccgag gggcatcgac ttctcgtgcg gcttccactc gtcgcgcccc tcggggacgc
     7021 gctccagagc gcggcgcgtc ccggcggcct ctcgttccag ctccggcaac aacagctcgg
     7081 cgatcgtcgg catcgtctct ctcctttccc tctcccggcg ccgctccccc ctaccccagc
     7141 cgcaacgccc gcttgatctc ctgagcccga gcctggatgg cggcccggtc gaggtcccgg
     7201 aacttctcgg ggaggagctc ccggctggtc gactccagga ccgcgaagag gatctgcatc
     7261 tcgcgttcca gcgagttggc cggagggatg aagtcgccga aggccttctt gatgtgctcc
     7321 tcggcaacct ccgacgcctc ggcggcctcg gcctccagca ccgcgcgcac cagcaccgcc
     7381 tcgaggtccg agccggagag gccgagtagc tgctcctcct ccaccacctt cgacagctcg
     7441 gtcaccggcg tcttcaggct cagcttcttg accatcacct ggtagatctc gtcgtactcc
     7501 tccaccgtct cggggtagaa gagcgggatg tgctcctcgg cgcggccctg gcgcttgagg
     7561 tcgatcggca gcaggtccgg gcgcgccgtg atcaggaacc agaggatctt gccgcggtgc
     7621 cgggtgtccg ccatgaagga ggcgatctgg gcgaagacgc gcgtctggac gccggagtcc
     7681 cccgactgct cgcggttgcc gagcgcggcg tcggcctcgt ccaccaccac gcccaccggc
     7741 gtcagcgacg aaaggatctt gaggatgcgc tccaggttcg attccgtggc gcccacccac
     7801 atcgaccgga agttcttgag cttgacgcag gggaagccga tctcgttggc gaagcactcc
     7861 acgaggaaag tcttggcgct cccaaccggg ccgcagacca gatagcccat cggcaccgcc
     7921 gccgggttcc cccgcgcgat cgcccgtgcc gcccggcgca gccggtcctt gaccgcgggc
     7981 atcccggcca ccatcgacag gtcgtacttg ggctcgacgt actcgagcag ccccagcccc
     8041 tcggtctcga tcacctcctt cttcacccgc cgcacctgct cgcgcgtcag cgtctcgtcg
     8101 tgggcgtcgg caccgtcgag catctggccg agctgcacgc gggtcatgcc ggcggtcagt
     8161 tcggcgaggc ggaccggcgg cagctccgag cgccgcttgt accagtcggc cgggcgcacc
     8221 gcctccaggt actccaggcg ctcccgcgga tcggggcgga cgatctccac ctcgcgcgtg
     8281 gcgtgggcgc gcagcagctt ggggtcgagc tcggccaggc tctcggtgat cagcacgatc
     8341 accatgttct tggcgagcag caccgggtcc tgcgaccact tgcgcaggta gaccaggacg
     8401 gcgcggtcct cgggtgtccg gtaggaggcg tcgccggcgg ggatcaccgt ctcggcgaac
     8461 ggcagcagca gcaccaccgg ccgcgacggc tggagcgagg agcagtgcct gaaataggag
     8521 tcgagcagcg cgaagcagtt ggcggcgtcg cgcggcagcg cctcggccca gttggtgccg
     8581 tgcaccgagt cgtagttgcg gagccgctcc tggaagtggc cgcgcgccgc cggggtcgag
     8641 aagcccagcc cctcggcgcg gttgtaggtg acgatcgact gccagccggc gaacagctcc
     8701 ttggtcacga actcgtcgag agagaggaaa cgcaggccgg aggagccgcg gaccggcacc
     8761 aggtcggccg gcacgccatg gaggacgaag gtattggcct gcttggccca gacggcgcgc
     8821 accagctccg ccccccaggg ggggagggac tcgacgaagg cggccggagg ggtggcgacg
     8881 gtggacatga gcggctccta gcgccgctgc ttcccccggg ccggaggtgg cggggggggc
     8941 ggcgcggtca tctgcttgat cttgccggag aggatcggct cgccggtctc cggcatggcg
     9001 ccgaacagcc gctccatctc ctcgacgctg gcggcgtcgg cctcggcgcg ggcgaggacg
     9061 tcgtcgatct ccagcgagaa ggcggtggca tcggtggcgg cgatcgattt ctggaggagg
     9121 agctggagca ggctctccag cgaatccacc tcggcgagca gcgcctggcg gttggcgacg
     9181 ttctggcgca ccgtgcgcag gagctgctcc ttgatctcca ggtgcttctc cagcgcctcg
     9241 cgcacccgct cgtcgacgtc gggcgcggcg agttgctcct tgagcttgcc gatgtcggcc
     9301 ctggcctgcg gctcgttgaa gctgcccacc atctcgtcgt acttctggat cagccagagg
     9361 tggcgcagac aggtggcgag gatcgagtcg aacttgttgt cctgatcggc gagcatcccc
     9421 tggctcgccg ccgacagccc cttgtagttg ttctggatcc gctcgcagag ctggcgcagg
     9481 cggacgaagc gcgccttggc cgacggggtc agcgcctcga agcgctgctc ctgcgacagg
     9541 gtctggaact ggcggtcggc gtacttcttc tcgatgtcca ggcggcgctt gatccgttcg
     9601 atcatgaacg gcgccgccat cagctcgagg ccggcgaggg ccaggagcgg caggggcgac
     9661 gccgtcaacg ccgagaaggc gaccgcgccg gtgaccagga tcgcctggta ctggttcgcg
     9721 accgccttct tgaggaccgg atgccgatcg aggaatccca cgtcggtacg gcgagcctcc
     9781 ggttagccgc cctctcgggc ccgctgcggc gtggcctcag cggtgggagt ggcggtggcg
     9841 gtgccgaacg gcgccgcctc gggggtggcg tcgggctgct tgagccccat ttcgacctcg
     9901 aactccgaga ggatgctctg ggcctggatc gaccgcgcct tgcgctccat cttgatgcgg
     9961 tcgacgtcga gcccgccggt cgccacctcg acggcggcct tggactcggc cacccgctgc
    10021 ttgatctcct cgaggtactt gtcggtcgtg gaggcggtac cgtaggtgtc gagctcggcc
    10081 agcgcgttgg cggcgctacg catgacctcg gccttctttg actcctggat cgccttgcgt
    10141 gcctggtcca tccgggcccg cagcttgccc ttgatgtcct cgacggagat ctgggcgttc
    10201 tggaaggcgt tctgcgccgc catgagctgc tcgctggtgg cctgcagatc ctggcggacc
    10261 gtctgcaggt ggacggcgtc ctcctcggcg aggcggcggg cctcggggct gccgtccttg
    10321 agcgcggcct ggatcgacga ggtgagctgc ttctccttgc gcaccagctc gtcgcgctcc
    10381 tgctccaggc ggatcaccgt ggcgcgggtg gtgacgagga tctggttgag gcgcggcagg
    10441 gtgttgcgca tctcctcgat ggactcctgg agcatcacct ccgggtcctc ggcgaaggag
    10501 atgaagtagc cgacccaaga cttgaagaca cgcgcgaggc gcttgaacat ggctatctcc
    10561 tagagactct ctaacgatcg cttccggtcg ccccttcgct cgctggcgtg ccggccggcg
    10621 ggctcgccgg ggcgggggtc ctgacggtgc tccccggcac gccttcggcg gagacggggt
    10681 tctcttcgac gaacggtccg acggattcgt acagccggcg ctcctcgatg cgtttgcgga
    10741 cctgccactg cccgaggcgg gcctgctcgg cctcgaccgc cttgcggttc gcctccatgg
    10801 tctcctgatg gcgccgcacc aactcgtcga tctcggcctg gaggcgggcg ttctcctgct
    10861 ccacggactg ggcgcgcgcg gcgagcttgc cgcgcagcag ctcctcgaac ttgtccaggg
    10921 cctggtcgcg ccgcaccgcg tcctggatga cgtcggcgat cggcaccggc ccggtggggt
    10981 tcatcttgag gaagccggcg agcgccgcgg cgcgcgcctt gtggtcgaga ccggcgaagt
    11041 ggtccgactc gaggatctcc agcaccttga aggcggtgta gccgtgcggc ggctcgctga
    11101 tcccggcggc cttgtagatc gcctcgaagt cggggatctc caggccagcc gtgccggcag
    11161 cggccggagc cccggcggtg gcaccgcttc ggggcagcgc ctgctcgtcg atccgcgcct
    11221 cagggatgga acccaggatc tcgcgcacgt cggtgggctt gcccggacgg gctgccggca
    11281 tggcgccgct accggcagcc ggatgcgaag cgcccgccgc cggactggcc gcggtgaccg
    11341 cgggggccgg ggagtcgagc tccacaatct tcatcctgct cagcacttcc ttgaggccca
    11401 tggctgctcc cgagaaatcc ttgaagtcaa agacggcgca ggcgcaagcc tatggccggg
    11461 ccgggggagt gtcaaggaca cgaatccctc ccccgcgtac cgcacgggcc agggcgcccc
    11521 gccttcctga aggagtgacg aaagccgggg aaacgtttcg cgggcccccg ccctcccctc
    11581 ccgcctacac ctcgtacgtc ggccgggcgt tcgggttgcg cggcaggcgc atgcgccgga
    11641 ggttggatag cggcacgagg gaggaggaga gcgggttgag ctccgccatc tcccgcgagg
    11701 tcagcggccg gatgctgaac acccggcggg gcacgcgggc ggtggtcggg tcacatcccc
    11761 gttccagcag ggccgcgagg atccagcggc cgatggtccg gcgtgcggcg ctcacgggag
    11821 ggattgtaca tgggggcttc acccttcccc ctcaccgttg gacaaggtat aagctcggac
    11881 gtgccctcgc agcaagattc gacgccgccc ccgcagtttc agtaccagga cttcatcatc
    11941 cagcttcgac tgctccggcc gggggagtac gaggtcaacg tacagtcacc cgccggcgag
    12001 gcccgcggcc tgttcgttc
//
LOCUS       DASPJI010000779         3053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_108374, whole genome shotgun sequence.
ACCESSION   DASPJI010000779 DASPJI010000000
VERSION     DASPJI010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108374"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3053)
                     /locus_tag="VEL74_21855"
     CDS             complement(<1..>3053)
                     /locus_tag="VEL74_21855"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYG65241.1"
                     /translation="DVTLAFAGAGFAEADPDSWSVDPARPQDYDALLAAVVRRSVPLT
                     RVVHLWSAPPFPVEEPYEAIQDAGFYSLIHLAQALGRRSGAPVHIDVVSSQVQSVHGG
                     DPLVPEKATLLGPCRVIPQEIARLTCRSIDVAWPGSDPSALVENLLAELALRGDEPVV
                     AWRGGERFVQDFQRERMEEVPGRPARLRDGGVYLLTGGLGGLGLAVAEHLARTVQAKL
                     VLTGRSPFPPREEWEGWLQTHGEEGDATSRRIRQLLDLEELGAEVLALSADVADAARM
                     AEVVAAARERFGALHGVIHLAGTPGGGILQLKTREAAARIVSPKVEGARVLDNLLAGQ
                     PLDFFVCFSSIASILGEFGQADYCGANAFLDAFAQRNARRGGPPFLTIDWDIWSEVGL
                     AVHTEVPAHLRPWRQEMLQQAIRPAEGTMAFQRLLERALGRVPQVVVSAQDLHGRIEL
                     GKSFTGESFLAELEKLEHHSHHPGEERPVRRVLGTVVAAAADGSLERRVAEVWQRVLG
                     VEQVGLHDNFFDLGGNSLLGLQLVSEIGRDLGVQVAPVTLFESPTVAALTRQIQPEPE
                     ADAALAAANAVKDELAERRRRLREGGGRAPVAIIGMAGRFPGAGNVEELWENLRDGVE
                     SVTFFSDDELRAAGVPEELLADPRYVRAGSVLKGIDQFDAGLFGYSPREAEVMDPQHR
                     IFLESAWETLERAGYDPSTYAGSIGVFAGSNLSTYLLQLYGDPDVKGSVNMLQAILGN
                     DKDSLTTTVSYKLNLRGPSVAVQTFCSTSLVAVHMACRSLRTGECDMALAGGIRVVTP
                     HHQGYLYEQGGIAPSDGHSRSFDAKANGSILGHGVGLVCLKRLDDALADGDPIHAVIL
                     GSAINNDGSLKAGYTAPSVAGQADAVMAAYEDAGVSPEDLSYVEAHGSATELGDPIEV
                     TALTKAFRTWTDRRQYIPIGSVKANFGHLDRAAGVTGLIKTVLAMQHEEIPPNILFDE
                     PNPKIDFAGSPFFVNTERRPWPSSPTAPRRAGVNSLGMGGTNVHV"
BASE COUNT          437 a         1054 c         1141 g          421 t
ORIGIN      
        1 tgacatggac gttggtgccc cccatgccca gcgagttgac gccggcacgg cgtggtgccg
       61 tcgggctgga cggccagggg cgccgctccg tattgacgaa gaacgggctg ccggcgaagt
      121 cgatcttcgg gttcggctcg tcgaacagga tgttgggcgg gatctcctcg tgctgcattg
      181 ccagcacggt cttgatcagg ccggtgacgc ccgccgcccg gtcgagatgc ccgaagttgg
      241 ccttgaccga gccgatgggg atgtactggc ggcggtcggt ccaggtccgg aacgctttgg
      301 tgagcgccgt gacctcgatc gggtcgccca gctccgtggc gctgccgtgg gcctcgacat
      361 aggagaggtc ctcggggctc accccggcgt cctcgtaggc ggccatgacc gcgtccgctt
      421 ggccggcgac gctgggagcg gtgtagccgg ccttgagcga gccgtcgttg ttgatcgcgg
      481 agcccaggat caccgcgtgg atgggatcgc cgtcggccag cgcgtcgtcc agccgcttca
      541 ggcagacgag gccgacgccg tggccgagga tgctgccgtt cgccttggcg tcgaaggacc
      601 gcgaatggcc gtcggacggg gcgatccccc cctgctcgta gaggtagccc tggtggtgcg
      661 gcgtcaccac ccggatgccc ccggcgaggg ccatgtcgca ctcccccgtc cgcaggctgc
      721 ggcaggccat gtggaccgcc accagcgatg tggagcagaa ggtctggacg gctacgctcg
      781 ggccgcgcag gttcagcttg taggagacgg tcgtggtcag cgagtccttg tcgttgccga
      841 ggatcgcctg gagcatgttg accgacccct tgacgtccgg gtcgccatag agctggagca
      901 ggtaggtcga gaggttcgag ccggcgaaga cgccgatcga gccggcatag gtcgacgggt
      961 cgtagcccgc ccgctccagg gtctcccagg cggactcgag gaagatgcgg tgctgcgggt
     1021 ccatgacctc ggcctcgcgc ggcgagtagc cgaagaggcc ggcgtcgaac tggtctatcc
     1081 ccttgaggac cgagccggcg cggacgtagc gcggatcggc cagcagctcc tcggggacgc
     1141 cggcggcgcg cagctcgtcg tcggagaaga aggtgaccga ctcgacgccg tcacgcaggt
     1201 tctcccacag ctcctcgacg ttgccggcgc cggggaagcg gccggccatg ccgatgatgg
     1261 cgaccggagc ccggccgccc ccctcgcgca ggcggcgccg gcgctcggcc agctcgtcct
     1321 tgacggcgtt ggcggcggcg agggcggcgt cggcctcggg ctcgggctgg atctggcggg
     1381 tgagggccgc caccgtcggc gactcgaaca gggtcaccgg cgccacctgg acgccgaggt
     1441 cgcggccgat ctccgagacg agctggaggc cgaggaggga gttccccccg aggtcgaaga
     1501 agttgtcgtg cagccccacc tgctcgacgc cgagcacccg ctgccagacc tcggccaccc
     1561 ggcgctcgag cgagccgtcg gccgccgcgg cgacgacggt gcccagcacg cgccggaccg
     1621 gccgctcctc tcccggatgg tgggagtggt gctccagctt ctccaactcc gccaggaagc
     1681 tctcgccggt gaacgacttg cccagctcga tgcggccatg gaggtcctgg gccgacacca
     1741 ccacctgggg gacgcgcccg agggcccgct ccaggagccg ctggaacgcc atcgtcccct
     1801 cggcggggcg gatcgcctgc tgcagcatct cctgccgcca gggccgcaga tgggccggca
     1861 cctcggtgtg gaccgccagg cctacctcgc tccagatgtc ccagtcgatg gtgaggaacg
     1921 gcggtcctcc ccgacgggcg ttccgctggg caaaggcatc gaggaaggcg ttggcgccgc
     1981 agtagtccgc ctggccgaac tcgccgagga tcgaggcgat cgacgagaag cagacgaaga
     2041 agtccagggg ctggcccgcg agcaggttgt ccagcacccg cgccccctcc accttggggg
     2101 agacgatgcg cgccgcggcc tcgcgcgtct tgagctgcag gatgccaccg cccggcgtgc
     2161 ccgccaggtg gatgacgcca tgcagggcgc cgaagcgctc ccttgccgcg gcgaccacct
     2221 cggccatgcg agccgcgtcg gcgacgtcgg cggagagggc gaggacctct gcccccagct
     2281 cttcgaggtc gaggagctgg cggatgcgcc gggaggtggc gtccccctcc tccccgtggg
     2341 tctgcagcca gccctcccac tcctcgcggg gcgggaaggg ggagcggccg gtgaggacga
     2401 gcttggcctg gaccgtccgc gccaggtgct cggcgacggc gaggcccaat ccgcccaggc
     2461 cgccggtgag caggtagacg ccgccgtcgc ggaggcgtgc gggccggccc ggcacctctt
     2521 ccatccgctc gcgctgaaag tcctgcacga agcgctcgcc gccacgccag gccaccaccg
     2581 gctcgtctcc ccgcagggcc agctcggcga gcaggttctc caccagtgcg gaggggtccg
     2641 aaccgggcca ggccacgtcg atgctgcggc aggtgaggcg ggcgatctcc tgcgggatga
     2701 cccggcacgg ccccagcagc gtcgccttct cgggcaccag gggatcgccg ccgtggacgc
     2761 tctgcacctg gctcgacacc acgtcgatgt ggaccggcgc gccgctgcgg cggccgagag
     2821 cctgggcgag gtggatcagg ctgtagaaac ccgcgtcctg aatggcctca tagggctcct
     2881 cgacggggaa cggcggcgcg gaccagaggt gaacgacgcg ggtgagcggc acgctccgcc
     2941 ggacgaccgc ggcgagaagg gcgtcgtagt cctgaggacg ggccgggtcc acggaccagg
     3001 agtcggggtc cgcctcggcg aagccggcgc cggcgaaggc cagagtgacg tcg
//
LOCUS       DASPJI010000780        10825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_505963, whole genome shotgun sequence.
ACCESSION   DASPJI010000780 DASPJI010000000
VERSION     DASPJI010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10825)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10825
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505963"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /gene="argS"
                     /locus_tag="VEL74_21860"
     CDS             complement(<1..1010)
                     /gene="argS"
                     /locus_tag="VEL74_21860"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HYG65242.1"
                     /translation="MWPELKQQLSREIARAIQERFGVEHTPVLEVPPRRELGDLASPA
                     AMQLARVLKRNPRAIAEELVQELTGELTGAGKLPPPVLGVRVEGAGYLNFSLDRAAFT
                     RAMLDAPLLSGGTGGSRHLQGGKVVIEHTNINPNKAAHIGHLRNAVLGDVLARALKGL
                     GYPVEVQNYIDDTGVQLADVVVGFMDLRGATIADIEAMPEPFDYLCWDLYSEVGRWYE
                     EDAERKALRRDTLHQLEIGQGERAEMGRLVARRIVRRHLATMARLDISYDLLTRESQI
                     LSLNFFAHAFERLKESGAVRLEKEGKNAGCWMMPLSQSDEFSGLEDPDKVIIRSDGTV
                     TYV"
     gene            1075..1620
                     /locus_tag="VEL74_21865"
     CDS             1075..1620
                     /locus_tag="VEL74_21865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HYG65243.1"
                     /translation="MTADNRMTVTLERTQLELIEGDITALDVDAIVNAANEHLQLGGG
                     VAGAIRQKGGPSIQEECDRIGGTPVGTAVMTGAGNLKARQVIHAVGPRWGEGDEDRKL
                     ASAVRAALALADRRGMRSIALPALSTGIFGFPLERAARVMLTEIHRYLQGGTKLERVV
                     ICLFGAEAFDVFRQELRRALR"
     gene            complement(1654..2079)
                     /locus_tag="VEL74_21870"
     CDS             complement(1654..2079)
                     /locus_tag="VEL74_21870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65244.1"
                     /translation="MSQGGATPPRQVVFDPTPEIFATDTAGSFTYPLLVAGKAFLGTS
                     SYDEVRFVFSIWHPSGQRTIDLDRAYVELRACFDPEEEHWTKIAEIEPVVPPYAGGDS
                     FDGWIVLPILGSSSSFAVVGSGFEPRARLQIRASAYFVA"
     gene            complement(2076..2777)
                     /locus_tag="VEL74_21875"
     CDS             complement(2076..2777)
                     /locus_tag="VEL74_21875"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HYG65245.1"
                     /translation="METTTVQTHPQIAASLRRAPVIGVVRTHSPEEAARQARLFAVSG
                     LELIEITFTVPGASTLVRQMLAKRDSSGPPWFGMGTVTTAARAREAVQAGAEFIVTPN
                     VSPEVAREARQAGLYLVMGALTPTEIVAARELGADLVKVYPLPPVGGPQYLSVVRQPL
                     GDIPMLAGGGFGIEEIPAYAQAGASAFGIGGPLLGTDEEETRRRIARALELARSAGGG
                     GRRHERHEKDEEETR"
     gene            2928..3482
                     /locus_tag="VEL74_21880"
     CDS             2928..3482
                     /locus_tag="VEL74_21880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG65246.1"
                     /translation="MAVPDKPLKGESLDQLLRRLRPRLKQVLGRYRIPAHDAEDLVQE
                     ALVSTIQKWDEIQDPEAWLMVTLRNRCVVYWRKRRNALYNSVDTAILELLSEPVAAPQ
                     EKAELLWDLNNLLEELPPRCRKLLRLRYGLGFDSAEVAEQMGYHPSSVRKVARRCMAA
                     LTFQMVTRGFTTAPSKPEDDDTEG"
     gene            complement(3562..4317)
                     /locus_tag="VEL74_21885"
     CDS             complement(3562..4317)
                     /locus_tag="VEL74_21885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HYG65247.1"
                     /translation="MESRIPPAVVLVRPREEGNVGAAARAMANMGLVRLILVEPAAEI
                     GKVATAFAHGAKHVLDGATRVASLDEALAPFRRVVGTTSMRDRRPDVPLLTPRELPAR
                     LAQDPPDTPTVLLFGPEVGGLSNDELARASLLVTIPCSPVQPTLNLSQAVLILAYELF
                     QDRLERGEAPAMPVSAATIEPPASAAEVDGLIGHAAEILRRVGFDRDDSFPGVLRDLR
                     ALAARASPGSREVAILRGICRRAQHALDRRPEE"
     gene            4399..4626
                     /locus_tag="VEL74_21890"
     CDS             4399..4626
                     /locus_tag="VEL74_21890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009566302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HYG65248.1"
                     /translation="MRETTFTELRNHAKTFFDAVEEGETVRVYRNGRPVADIVPVKTA
                     TPAWKNPPRARLSLGNLSLADEVLADRDENR"
     gene            4623..5057
                     /locus_tag="VEL74_21895"
     CDS             4623..5057
                     /locus_tag="VEL74_21895"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG65249.1"
                     /translation="MRVFLDTSALTKRYVEEDRSEQVRSLCAQADALGVSVLVVPELI
                     STLCRLVREGRLSPGDYRSLKTAVEVDLTDADLCDLSQEAFDQTLRCLERHPLRALDA
                     LHVGSALVYQPDLFVTADRRQAEAADGEGLAVMDLSRALAGR"
     gene            complement(5619..5909)
                     /locus_tag="VEL74_21900"
     CDS             complement(5619..5909)
                     /locus_tag="VEL74_21900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65250.1"
                     /translation="MPDLPQSAPLDLDRFSEALRRWLYDRVVRIHFDRSDTTVPYDPD
                     AAGLQVICLGGRWFAVWRDLEEPPSAPERLRLQVVGIRASPDDPGDIELYAV"
     gene            6139..6606
                     /locus_tag="VEL74_21905"
     CDS             6139..6606
                     /locus_tag="VEL74_21905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG65251.1"
                     /translation="MEMLRIFVRTLGLGNVEIARRTKIAHANVGRYFRGEARVPLDFV
                     IAVIRALGLEYREFFELAYADQPAQPTEARKKIERILEVLPARRPAASVVEPEPEPEP
                     PKTDVERLLEAFRREVGNLLDARQSSQVIAPPPAPVPEPEPAKPASRRRKKEA"
     gene            complement(6654..7853)
                     /locus_tag="VEL74_21910"
     CDS             complement(6654..7853)
                     /locus_tag="VEL74_21910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65252.1"
                     /translation="MGERDHLAQGEGTAESRHAAGFDVPLHTRLLRQLSKSERACYQT
                     ALALLSSGRGLLALAREGDLPLAGLGVYEALLTRSWEIRFQDKEEMISLANAAVEVAA
                     RFDSEALGAQQVADLRARAWGELANSYRAADQLRSADRAFGQAFHFQTLGTDDPYLKA
                     RLFDLSASLLGTWRDFALAEARLRAAADLYLELGEPHLAGRAFISQALYTFYSCRPEE
                     ALRLNQQGKKLINPRRERSLFLMAVHNDLLFLVDLGRYTEAKRVLFDSRQHFIYQDRF
                     NALRLRGIEGRIEYGLGKLLSAEAAFRETRDGFDEAGAPFLAAIARLDLAMVLVSQSH
                     LDDAEQEVLAARNLFKSVEAYREFLGTMVYLEEVFRRRTVTPALIQNSIAEIWRKNLQ
                     VGPRHFR"
     gene            complement(7900..8205)
                     /locus_tag="VEL74_21915"
     CDS             complement(7900..8205)
                     /locus_tag="VEL74_21915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65253.1"
                     /translation="MSRRRVSRLVLVVLLVLPFAGAVRAEASELSGPRLTWDWLAGLW
                     EDGISAWWPWSDTTQEKAAGALDPNGGTATATGTTPPPTNGPGQGEQGYGIDPNGTP"
     gene            8536..8781
                     /locus_tag="VEL74_21920"
     CDS             8536..8781
                     /locus_tag="VEL74_21920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65254.1"
                     /translation="MNTNQSVVVTPRTDVQPLDTSGLHVSLKAERVQEPRAAADDAVY
                     LYEFAVNRLQPVTIEVPLVVVTFHGQPSWDVPGGKTR"
     gene            8848..9222
                     /locus_tag="VEL74_21925"
     CDS             8848..9222
                     /locus_tag="VEL74_21925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65255.1"
                     /translation="MEIMQNAVPATNAAPAKKLIRPRGEKSLARKLETRLKAERVQEK
                     LRAAPGWRLLAGGKAVDRVREFHDPRVAAAWAQFVHLYAERAGFPAEVTVSGARVMVI
                     LRDRVTRGELSAAALEAVKVLG"
     gene            9274..9567
                     /locus_tag="VEL74_21930"
     CDS             9274..9567
                     /locus_tag="VEL74_21930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65256.1"
                     /translation="MPIISSYADIIRDWEALLVAIIDKLAHMPEKLTEGRATAIRLRG
                     AIKAALGHTHEGLVQYGMVPRRHRVRRPKLVEAPTAEDANRTAPPSVEGEPIH"
     gene            complement(9614..10642)
                     /locus_tag="VEL74_21935"
     CDS             complement(9614..10642)
                     /locus_tag="VEL74_21935"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HYG65257.1"
                     /translation="MSFFMLLFLAGVAAPPPPIPPTTQPVSTPVRLATPAFGGTVDIE
                     VRDLPGDAARGAIEAAFAEVAEVERLLSLSASVPGMPGGVAALNAAAGRGPQGVDPRL
                     FSLLTKAADFCTWSEGAHGPLGRDLYTLWGARTPVEAPPDIERLGDAVGLAGCNRLAL
                     DPKAGTAALAEGAAVEPRGFAEGFAVDRAVEVLRQRGVKNGFVQIGAIRRGFGAGPQD
                     KGWPVMLPPFPDLGEPMGRVYLRDQALVVATASDSPLRVGSGTVFPYISHRSGQPAQG
                     VVAVLAATDLALDAQALTAALAILGPREGRLRLGGLRPQPSALWLLGSGEGAPLLVGH
                     RWSEVPKR"
BASE COUNT         1765 a         3644 c         3822 g         1594 t
ORIGIN      
        1 acgtaggtca ccgtgccgtc cgagcggatg atgaccttgt ccgggtcctc gaggccggag
       61 aactcgtcgc tctgcgacag gggcatcatc cagcagccgg cgttcttccc ctccttctcc
      121 aggcggaccg cgccgctctc cttgagccgc tcgaaggcgt gggcgaagaa gttcagcgac
      181 aggatctggc tctcccgcgt cagcaggtcg taggagatgt cgagccgcgc catggtggcg
      241 aggtggcggc ggacgatgcg ccgggccacc aggcggccca tctccgcccg ctccccctgg
      301 ccgatctcga gctggtgcag ggtgtcgcgg cgcagcgcct tgcgctccgc gtcctcctcg
      361 taccagcggc cgacctcgct gtagaggtcc cagcagaggt agtcgaacgg ctccggcatc
      421 gcttcaatgt cggcgatcgt ggcgccgcgg aggtccatga accccaccac cacgtcggcg
      481 agctggacgc cggtgtcgtc gatgtagttc tgcacctcga ccgggtagcc gagccccttg
      541 agggcgcggg ccagcacgtc gccgagcacg gcgttgcgca ggtggccgat gtgcgccgcc
      601 ttgttggggt tgatgttggt gtgctcgatg accaccttgc caccctggag atggcgggac
      661 ccgccggtcc cccccgatag caggggggcg tcgagcatcg cccgggtgaa ggcggcgcgg
      721 tcgagcgaga agttgaggta gcccgccccc tcgacccgca cgcccagaac cggggggggg
      781 agcttgccgg cgccggtcag ctcgccggtc agctcctgga ccagctcctc ggcgatcgcc
      841 cgcgggttgc gcttgaggac ccgggcgagc tgcatggcgg cgggggaggc gaggtcgccc
      901 agctcgcgcc gcggcggcac ctccaggaca ggggtgtgct cgacgccgaa gcgctcctgg
      961 atggcgcgag cgatctctcg tgacagctgc tgcttgagtt ccggccacat ggcggcaggg
     1021 agtctacacc atgagagaag gatgcccttg gtatcctttt ccgggagagt caggatgacc
     1081 gccgacaaca ggatgacggt gaccctggag cggacgcagc tcgagctgat cgagggggac
     1141 atcaccgctc tcgacgtgga cgccatcgtc aacgccgcca acgagcacct gcagctgggg
     1201 ggcggcgtgg ccggagcgat ccggcagaaa ggggggccgt cgatccagga ggagtgcgac
     1261 cggatcggcg gcacgccggt ggggacggcg gtgatgaccg gcgcggggaa cctcaaggcc
     1321 cggcaggtca tccacgccgt gggcccgcgc tggggggagg gggacgagga caggaagctc
     1381 gcctcggcgg tgcgcgccgc cctcgccctc gccgaccgcc gggggatgcg gtcgatcgcc
     1441 ctgccggcgc tctccaccgg catcttcggc ttccccctgg agcgggccgc ccgcgtcatg
     1501 ctgacggaga tccaccgcta cctgcagggg gggacgaagc tggagcgggt ggtgatctgc
     1561 ctcttcggcg ccgaggcctt cgacgtcttc aggcaggagc tgcggcgggc gctccggtaa
     1621 cctccggtcc ccggaaaggc cgccgcagcc ctctcaggcc acgaagtagg cgctggcgcg
     1681 gatctgcagc cgggcgcgtg gctcgaagcc gctgcccacc acggcgaagg agctggagct
     1741 gcccaggatg gggaggacga tccagccgtc gaagctgtcg ccgccggcgt agggggggac
     1801 gaccggctcg atctcggcga tcttggtcca gtgctcctcc tcggggtcga agcaggcgcg
     1861 cagctcgacg taggcgcggt cgaggtcgat ggtgcgctgg cccgaggggt gccagatgga
     1921 gaagacgaag cgcacctcgt cataggagct ggtgccgagg aacgccttgc cggcgaccag
     1981 cagcggatag gtgaaggagc ccgcggtgtc cgtggcgaag atctccggcg tggggtcgaa
     2041 caccacttgg cggggcgggg tcgctcctcc ctgactcatc tcgtctcctc ctcgtccttc
     2101 tcatggcgct catggcgccg tccgccgccg cccgctgaac gggccagctc gagcgcgcgg
     2161 gcgatgcgcc gccgggtctc ctcctcgtcc gtccccagga gcggtccgcc gatgccgaag
     2221 gcgctggcgc cggcctgggc gtaggcgggg atctcctcga tgccgaagcc gccgccggcc
     2281 agcatgggga tgtcccccag cggctggcgc acgaccgaga ggtactgcgg cccgccgacc
     2341 ggcggcaggg ggtagacctt gaccagatcg gcgcccagct cgcgggcggc cacgatctcg
     2401 gtgggggtga gcgcccccat caccagatag aggccggcct gccgcgcctc ccgcgccacc
     2461 tcggggctga cgttgggggt cacgatgaac tcggccccgg cctggaccgc ctcccgcgcc
     2521 cgggcggcgg tggtcaccgt ccccatcccg aaccaggggg gaccggaaga gtcccgcttt
     2581 gccagcatct ggcgcactag agtagaagct cccggtaccg tgaaggtgat ctcgatcagc
     2641 tccaggcctg atacggcgaa gagcctggcc tggcgtgccg cctcctcggg ggagtgcgtc
     2701 cgcacgacgc cgatgaccgg cgcccggcgg aggctcgcgg cgatctgggg atgagtctgg
     2761 acagtcgtcg tctccatgag gaggtggagt ctatcatcgt cctcgcggaa gggacgccgg
     2821 gtctgtaacc agggtgtcag aggccccggg tgggcaagaa ggcccttccg ggttattatt
     2881 gctgctcgac tatttggagc cgacagctct tcgggcaagt gggatccatg gcagttcctg
     2941 acaaacctct caagggagag tcgctggacc agctcctgcg acgtctccgt ccccggctga
     3001 agcaagtcct cggtcgctac aggatccccg cgcacgatgc cgaggacctg gtgcaggagg
     3061 ccctcgtctc gacgatccag aagtgggacg agatccagga ccccgaggcc tggctcatgg
     3121 tcaccctgcg caatcgctgc gtcgtgtact ggcgcaagcg ccgcaacgct ctctacaact
     3181 cggtggacac ggccatcctg gagctcctgt cggagccggt ggccgcgccg caggaaaaag
     3241 ccgagctcct gtgggacctc aacaacctcc tggaggagct gccgccccgc tgccgcaagc
     3301 tcctgcgcct gcgctacggt ctgggcttcg actccgccga agtcgccgag cagatgggct
     3361 atcacccctc cagcgtccgc aaggtggccc gccgctgcat ggcggccctg accttccaga
     3421 tggtcacccg cggcttcacg accgccccca gcaaacccga ggacgacgac accgagggct
     3481 gattcgcccc cgccggtctt cgtccgcctc ctctcttctg ccagtctccg ggtaggtggg
     3541 gtgtcgtgcg cccgcagggc gtcactcctc cggccgccga tcgagggcgt gctgcgcccg
     3601 ccggcagatg ccgcgcagga tcgccacctc gcggctgccg ggggaggcgc gcgccgccag
     3661 cgcccgcagg tcgcgcagca cccccgggaa ggagtcgtcg cggtcgaagc cgacgcgccg
     3721 caggatctcc gccgcgtggc cgatgaggcc gtcgacctcg gcggccgagg ccggcggctc
     3781 gatcgtcgcc gccgacaccg gcatggccgg cgcctccccc cgctccaggc ggtcctggaa
     3841 cagctcgtag gcgaggatca gcaccgcctg gctcaagttg agggtcggct gcaccgggga
     3901 gcaggggatg gtgaccagca ggctggcgcg ggcgagctcg tcgttgctga gccccccgac
     3961 ctcggggccg aagagcagga cggtcggggt gtccggcggg tcctgggcca gccgggcggg
     4021 gagctcccgc ggagtgagga ggggcacgtc cggccgccgg tcgcgcatcg aggtggtgcc
     4081 caccacccgc cggaacggcg ccagcgcctc gtcgaggctc gccacccgcg tcgccccgtc
     4141 gagcacgtgc ttcgccccgt gggcgaacgc ggtcgccacc ttgccgatct cggccgccgg
     4201 ctcgaccagg atcagccgca cgagccccat gttcgccatc gcccgcgccg ccgccccgac
     4261 gttgccttcc tcgcgcgggc ggaccagcac gacggcgggg gggattcggg attccatggg
     4321 gggactctat caatccgggg aaggcagggt atgtacagat caatggacag agacctgccg
     4381 gtctgagaga atggcggcat gcgcgaaacg acgttcaccg agttgaggaa ccacgccaag
     4441 acctttttcg acgccgtcga ggagggggag accgtccgtg tgtaccgcaa cggccggccg
     4501 gtcgcggaca tcgttccggt caagacggcg acgccggcgt ggaagaatcc acctcgggcg
     4561 cggctctctc tgggcaatct cagcctcgcg gacgaggtgc tggccgaccg cgacgagaac
     4621 agatgagggt ctttctcgat acctccgcgc tgaccaagcg ttacgtggag gaggacaggt
     4681 cagagcaggt gaggtccctc tgcgctcagg cggacgccct gggggtcagc gtcctcgtcg
     4741 tccccgagct gatctcgact ctttgccgtc tggtgcgcga gggacggctc tcgccggggg
     4801 attaccggag cctcaagacc gccgtggagg tggacctcac ggatgccgac ctctgtgacc
     4861 tctcccaaga ggctttcgac cagaccctcc gctgtctcga acgccatccc ctgagagcgc
     4921 tcgatgcgct tcacgtcggg agcgccctcg tctaccaacc ggacctcttc gtcaccgcgg
     4981 accgccgtca agccgaggcc gccgacggtg aagggctcgc cgtaatggat ctctcccgcg
     5041 ccttggcggg ccgctagcac ctcgcgccgc ccatctatcc atctatataa ggaaggagcg
     5101 ccttctccgc cgccgtgggg aaggcatcgt cgtctcgccg gagagtccgg cggcgcgttg
     5161 tgagctccga cggcggattg gacacttccg gtggctcgcc gaagcctccg acggcgatcc
     5221 ggagcctacg gcggcccgcc ggaaccttcg gcggctcgcc ggagactacg gcagcttgtc
     5281 ggagcttccg gtggctcatc gaagactgcg acggcccggc ggaagcttcg gcggcctctc
     5341 ggaagcttcg gcggcctctc ggaagctctg acggcccatc ggagatttcg gcggcagggt
     5401 ggaaccttcg gcagcgcgcc ggaagcttcc gatgcccgtc gaagactccg acagcctgcc
     5461 gaagcgggag gcggcggggt cggtggccct cgagcgcagc ggcgccattc tggatgagcg
     5521 gaccggaacc accgggtagg ccgctgcatc cttccttata gagcgggatc ggggggcgcc
     5581 ctcggaaacg ggggaacccc ccgatcagtc tccctccctt acaccgcgta cagctcgata
     5641 tcccccggat cgtcggggct cgcccggata cccacgacct ggaggcgcag gcgttcaggg
     5701 gcgctcgggg gctcttccag gtctctccag acggcgaacc atctccctcc gagacagatc
     5761 acctggagcc cggcggcgtc gggatcatag gggacggtcg tgtccgagcg gtcgaagtgg
     5821 atccggacca cccggtcata cagccagcgg cggagcgcct cgctgaaccg gtcaaggtcg
     5881 agcggcgcgg actgaggcag gtcgggcata ggtctcctca tggattcgcg ggctcctctt
     5941 tctggaaagc ccgccccggt cgtttcgcaa gggcgggatg agttctcgcc ccaggacgcg
     6001 ctttgtagca gcggcgaccg atttgagtca atagatcgga aacggaaccg ttgaccctga
     6061 agggggagag cggctatact ccgggccatg ccaaaacgag atacgaagga ccctatccgg
     6121 caggaaaccg aacggctgat ggagatgctc cggatcttcg tccggaccct cggcctaggc
     6181 aacgtggaga tcgcgcggcg gacgaagatc gctcacgcca acgtcggcag gtacttccgg
     6241 ggggaggccc gggtgccgct cgacttcgtg atcgccgtca tccgcgcgct cgggttggag
     6301 taccgcgagt tcttcgagct ggcctatgcc gaccagccgg cgcagccgac ggaggcgcgg
     6361 aagaagatcg agcggattct ggaggtcctc cccgcgcggc ggccggcggc ctcggtggtg
     6421 gagccggagc ccgagccgga gccgccgaag acggatgtcg agaggttgct cgaggcgttt
     6481 cggcgcgagg tggggaacct gctcgacgcc cggcaatcca gccaggtcat cgcgcccccg
     6541 ccggcaccgg ttcccgagcc cgagccggcg aaaccggcct ccaggaggcg aaagaaggaa
     6601 gcctgaagcc gggagtgtgg cccacaagtg agcccgggtc tttctgaccg ggctcaccgg
     6661 aagtgtcgcg ggcccacctg gaggtttttg cgccagatct cggcgataga gttctggatg
     6721 agcgcaggcg tcaccgtgcg gcgccgaaag acctcttcca ggtagaccat cgtgcccagg
     6781 aactcgcggt aggcctcaac actcttgaag aggttccggg ctgccaggac ttcctgctcg
     6841 gcatcgtcaa ggtgactctg gctgacgaga accattgcga ggtccaggcg agcgatggca
     6901 gcaaggaacg gggcgccggc ctcgtcgaac ccatctctgg tttcacggaa cgctgcttca
     6961 gcgctaagca gcttccccaa gccatactca atgcggcctt caattccacg gagtctgaga
     7021 gcgttgaagc ggtcctggta gatgaagtgc tgtcggctgt cgaacaggac cctcttcgcc
     7081 tcggtgtatc gcccaagatc gaccagaaat agcaggtcgt tgtgcaccgc cattaagaag
     7141 agtgagcgct cccggcgtgg gttgatgagt ttctttccct gctgattgag ccggagcgcc
     7201 tcttcgggac gacagctata gaaggtgtag agcgcttggc tgatgaaggc gcgacccgct
     7261 aagtggggtt ctccgagttc caggtagagg tcggcggcgg ctcgaagccg ggcttcggcc
     7321 agcgcaaaat ctcgccaagt ccccaagagg gaggcgctga ggtcaaagag ccgcgccttc
     7381 aggtatgggt catcggtgcc cagggtctgg aagtgaaagg cttggccaaa ggcgcggtct
     7441 gcggagcgca gctgatcggc cgcgcggtag gaattggcta gctcgcccca ggcccgtgcc
     7501 cgcaggtcgg cgacctgttg tgctccaagg gcctcggaat cgaacctcgc tgctacctcg
     7561 accgctgcat tggccagaga gatcatttcc tctttatctt ggaatctgat ctcccagctt
     7621 cgagtgagga gagcctcata aacaccgagt ccggccaagg gtaggtcgcc ttcgcgcgcc
     7681 agggccaata agcccctacc cgaggagagt agggccaatg cagtctgata gcaggcgcgc
     7741 tcgctcttag agagctgtcg cagcaagcga gtgtgcagag gcacatcaaa tccggccgca
     7801 tggcgggact cggcggttcc ttcgccctgt gcgaggtgat ctcgttcgcc caagccgtct
     7861 ctccaagttg ggcgcacatc atctcacagg gcgggcgagt tatggagtcc cgttgggatc
     7921 gatgccatag ccctgctcac cctgcccggg accattggtc ggaggagggg tggtgccggt
     7981 ggcggtcgcc gtgcctccgt tgggatcgag ggcgccggct gccttctcct gggtggtgtc
     8041 gctccagggc caccaggcgg agatcccgtc ttcccagagg ccggccagcc aatcccaggt
     8101 gaggcgggga cccgagagct cggacgcctc ggcgcggacg gctccggcga acggcaggac
     8161 gagcaggacc acgagcacca ggcgagagac cctacgacga gacatacctc tcctccttcc
     8221 ccgcggcacg agccggaccc gcatggccag gtgtccgcgg cgattccctg aagggacctt
     8281 cgcagatacg gtgctcccgg acaacgggga ggggacaggg ccaggacata tccccatgat
     8341 tgcacagggc tcaacctgtc tgacagaccg aattaggtac gcagagccgt aaaatctcga
     8401 aaaagttcgc caaaacccct tgacaggccg cggaccggtg cctaccatca ctccgcgacc
     8461 aaaaacggtc ctaaacatcg atccaagatg gccggtgcca atgcaggcgt ccggattcca
     8521 gaggagagaa gcagcatgaa cacgaaccag tctgttgtcg tcaccccccg gaccgacgtc
     8581 cagccgctcg acacgagcgg gctgcacgtg agcctcaagg cggagagggt gcaggagccg
     8641 cgggcggcgg cggacgacgc cgtctacctg tacgagttcg cggtgaaccg gctccagccg
     8701 gtgacgatcg aggtcccctt ggtggtcgtc accttccacg ggcagccgtc gtgggacgtt
     8761 cccggcggga agacccggta agccagaagc aaacctgttg accttccgga gccatggagg
     8821 ctccggatct ttgaaaggag aagccgtatg gaaatcatgc agaacgccgt ccccgccacc
     8881 aacgccgcgc cggcgaagaa gctcatccgg ccgcggggag agaagtcgct cgcccggaag
     8941 ctggagaccc gcctcaaggc cgagcgggtg caggagaagc tgcgcgcggc gccgggctgg
     9001 cgcctgctcg ccggcggcaa ggcggtcgac cgggtgcggg agttccacga cccgcgggtg
     9061 gccgccgcct gggcccagtt cgtccacctc tacgccgagc gggccggctt cccggcggag
     9121 gtgaccgtct ccggagcgcg ggtgatggtc atcctgcgcg accgggtcac ccgcggcgag
     9181 ctgtccgccg ccgcgctcga agcggtcaag gtcctcggct gaccgtcaac cgggggaaca
     9241 ctcaaacgca agggaacaga acggaaccga atcatgccca tcatctcgtc gtacgccgac
     9301 atcatccgcg actgggaggc cctcctggtg gcgatcatcg acaagctcgc gcacatgccc
     9361 gagaagctga ccgagggccg cgccaccgcg atccgcctgc gcggcgccat caaggcggcc
     9421 ctgggccaca cccacgaggg gctcgtgcag tacggcatgg tgccccgccg ccaccgggtc
     9481 cgccgcccca agctggtcga ggcgccgacc gccgaggacg ccaacaggac ggccccgccc
     9541 tccgtggagg gtgagcccat ccactagccg ctgcaccggg gggtgtgccg gacggcacgc
     9601 ccccctttct tctttaccgt ttgggaactt cggaccagcg atgaccgacc aggagaggcg
     9661 ccccctcgcc gctgccgagc agccagagag ccgacggctg gggccgcagg ccgccgagac
     9721 gcaggcgccc ctcgcggggg ccgaggatgg cgagggccgc ggtgagcgcc tgggcgtcca
     9781 gcgccaggtc cgtggcggcg aggaccgcca ccaccccctg cgccggctgg cccgagcggt
     9841 ggctgatgta ggggaagacg gtgccgctcc ccacccgcag aggggagtcc gaggcggtgg
     9901 cgaccaccag cgcctggtcg cgcaggtaga cgcggcccat gggctcgccc aggtccggga
     9961 acggcggcag catcaccggc caccccttgt cctgcgggcc ggcgccgaag ccgcggcgga
    10021 tggcgccgat ctgcacgaag ccgttcttga ccccgcgctg ccgcagcacc tccaccgccc
    10081 ggtcgacggc gaaaccctcc gcgaagccgc gcggctcgac cgcggcgccc tccgccaggg
    10141 cggcggtgcc ggccttgggg tccagggcca gccggttgca gccggcgagc cccaccgcgt
    10201 cgccgaggcg ctcgatgtcc ggcggcgcct ccaccggcgt gcgcgctccc cacaaggtgt
    10261 agaggtcgcg gccgagcggg ccgtgggccc cctccgacca ggtacagaaa tccgcggctt
    10321 tggtgagcag cgagaagagg cgcgggtcca ccccctgggg gccgcggccg gcggcggcgt
    10381 tgagggcggc gaccccgccg ggcatgccgg gaacggacgc ggagagggag agcaggcgct
    10441 cgacctccgc cacctcggcg aaggctgcct cgatggcgcc ccgggcggcg tcacccggga
    10501 ggtcccggac ctcgatgtcc accgtgccgc cgaacgcggg agtcgcgaga cggacgggtg
    10561 tggagacggg ctgggttgtc ggagggatcg gcggaggcgg agccgccacc ccggccagga
    10621 agagcaacat gaaaaaagac atggagggat actacccgcc ggaggcgggc tgcgccagga
    10681 agcactcgag cacggcgctg taggtggcgc gcaggtcttc cgggctgtag tccgccggga
    10741 agtcgatccg ctcggcgagg agctggtcgc gcagcagccc ctcgaaggcg ccgaccaggg
    10801 ccgaccgcat ggcctgcacc ttgaa
//
LOCUS       DASPJI010000781         7765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_794784, whole genome shotgun sequence.
ACCESSION   DASPJI010000781 DASPJI010000000
VERSION     DASPJI010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7765)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794784"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="VEL74_21940"
     CDS             complement(<1..1661)
                     /locus_tag="VEL74_21940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYG65258.1"
                     /translation="MTAGRLLSSRVHLVWSAVSLLVLSLCLYYGYVATVPKISFEANS
                     RWWVIVADPCPAGRDCVQIGDQILELGGVTLQEFKNDWAIDMLDLFGRDGTAQVLVRR
                     GGEVRMVEAYAFSGIDLLWRIETITSLLPLVFWLMGTVAVLLLRPRDERWVALVLFSY
                     VTALWFSSGLASGRHTGGAAYVFRTVIWFFLPLTVHLHTLLPNYLVRRRVRLLAPLYG
                     LSAALMVLDHLNFLPRLERAYLASFLVGAIVSPLLLLARMFLRVDPAVRLATRIMLYG
                     LALGFGPIILFFIALPKILGDPAVAMDARSLYPWMVGIALIAIPMMPMSYVYAIYKHH
                     LGSFEFRANRLLGAYSFFLTYVTLYLLAMSVVSSRWESDIRRFSTATLLVSLLFVAVA
                     SMLRNRFQSLVDRHVLGIRHTAEEVIGIVSERIATAFDRAILTEVIVEEILPTLLVRR
                     SALYLFAGQGGELERVYERGLPAGAAPPDADQTRELLARSGRYLPADQETGSPLPWVR
                     VVLPLTLQGETLGVWLIGRRDPDDFFPASDIHLMSTIANQVAAVL"
     gene            complement(1658..2614)
                     /locus_tag="VEL74_21945"
     CDS             complement(1658..2614)
                     /locus_tag="VEL74_21945"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYG65259.1"
                     /translation="MRIERIKTGVRSLPEVAAWPQMTDLVDRAVHREWRSVWEYPVAA
                     CLAVGGEEEAAAPAAAAVFCSMTSIHLVDDMLDEDPQGDYRRLGSGIAANLALAFQAA
                     AHRLLDDPGVAPAIRAALQAKLGGMALATAFGQNLDARHLSSEEEYWRVLEAKTPPLI
                     GAALHMGALLGGATAETAEGLEEIGRIMGRFIQVSDDLSDALKAPAGADWQRRSHNLA
                     LLYAMTAEHAERSEFLHLSTRAAEPAALAAAQTILVRCGAVSYCTLRMLEFSHAARER
                     LSRLQLADRKPIERLLEIQAKPLAHLLESVGMAEPALTFEGG"
     gene            complement(2770..2919)
                     /locus_tag="VEL74_21950"
     CDS             complement(2770..2919)
                     /locus_tag="VEL74_21950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65260.1"
                     /translation="MSIRDFHSKFASATPENALFAALRRLSESTDLKSLVTTQDFAWG
                     DWTVE"
     gene            complement(3170..4123)
                     /locus_tag="VEL74_21955"
     CDS             complement(3170..4123)
                     /locus_tag="VEL74_21955"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYG65261.1"
                     /translation="MRLDELKQRILALLEVSAWPQMVRLVERAVRDEPQSIWEHPAVA
                     CRAVGGSAEAALPGAAAIVCSLLSIHLVDDILDEDPRGDYREVGEGPAANLALAFQAA
                     AHRLLENASPNPETRAALHSRLARMALATAYGQSLDVQGASNEEEYWRVVETKAPPLF
                     AAALGIGALLGGASDVTAEQLERLGGHLGLFSQISDDLVDALQVPARPDWRRPFNNLP
                     LLYALTVEHPERDRFLQLFERLDQPGALSEAQAILLRCGAVSYCAFRLAEISRQARAV
                     FAGIPLDDPEPLERLLDHFLTPLDELLATMGFAGSPSGEPN"
     gene            complement(4225..4422)
                     /locus_tag="VEL74_21960"
     CDS             complement(4225..4422)
                     /locus_tag="VEL74_21960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65262.1"
                     /translation="MSIHEFYSKFVSADEKTPLFTALRRLSSLGDTGGLIRQMVATGW
                     PSWDSPPQGGSTAVGQVIGHA"
     gene            complement(4640..5878)
                     /locus_tag="VEL74_21965"
     CDS             complement(4640..5878)
                     /locus_tag="VEL74_21965"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HYG65263.1"
                     /translation="MLRVLVLDDESMVVDLLAVCLRAPGVHLTTCLEIEAAEALLRCF
                     RFDVVVTDLSVSGLGGLEGMRLIRFITTHFPDITVYVLSGFVDDRVRALCAALGVTAV
                     LEKPGGLSELRDLLLERREALAPAESGGGEARTAVPPGEVQKVELLEEFLATSTIASV
                     LQPLVRLRQGAEPFEVFGVEGLARGPSASVLGSPAVLLAYAAHKELLFKVDMICIQAA
                     LAEVRHLGIPVATFLNVQPRSMTNPVFAAKLSEAVRGAGLAEKDIVLELTEQQTILNP
                     RAFATTLQKLRERGFRIALDDFGEGSSNLNLFQDLRPDFLKISGTFCRNLARDPFKQI
                     VVQSTAEMAARAGTATVMEAVETAEDAEVLRHLGIDYGQGHYFSEPLPGRELAGRLHG
                     GQAYPRPGGADLSRTAAPPS"
     gene            complement(5939..7573)
                     /locus_tag="VEL74_21970"
     CDS             complement(5939..7573)
                     /locus_tag="VEL74_21970"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYG65264.1"
                     /translation="MSDATKNLYESILLQISDSAARGGALSPLPPARASAAVVPWRRR
                     DAGGIEVFWIQRAEALPFMGGWHAFPGGALSRADAGVPVAGEVAGAGGTGDAPAAGLP
                     ESMQGDAEGPDLVPGLVACAIRELFEETGLLLATPSGNAPSSEDLAEARRALLAGERP
                     FAELMADLGLTPDASRLVWAGRWMTPPFAPLRFDNRFFLLEWPGSEAVQPSVLPGECA
                     AGEWTEPGAAWRRWRSGDVLAAPPILHILEVLAENGPAAHGLERLRDPSETNLGPLRR
                     VEMRPGVVMFPLLTHTLPPAATTNAYLLGTGEAVLVDPGSTLEVENGRLERALAAARD
                     RLGRRVTAIWLTHHHPDHVGGVERLRDTLGVPVLAHPATAERLAGRVRVDGELVDGQR
                     VVLAGDPPCAVRVVHTPGHARGHLCFFEEDQRSLLAGDMVAGIGMIVVDPPEGDMDDY
                     LESLEKMIALAPRTLFPAHGPVVRDAVGKLREYVQHRLWREEKILAAWNEGRREPREM
                     LPTVYEDVPPEAHGLAQRQILAHLERLRRAGRLAGA"
BASE COUNT         1232 a         2619 c         2868 g         1046 t
ORIGIN      
        1 tcgagcaccg ccgccacctg gttggcgatg gtcgacatca gatggatgtc gctcgccggg
       61 aagaagtcgt cggggtcgcg gcggccgatg agccagaccc ccagcgtctc cccctgcagg
      121 gtcaggggga ggacgacgcg cacccagggg aggggggagc cggtctcctg gtccgcgggg
      181 aggtagcggc cgctgcgcgc caggagctcc cgcgtctggt ccgcgtcggg cggcgcggcg
      241 ccggccggca ggccgcgctc gtagaccctc tccagctccc ccccctgccc ggcgaagagg
      301 tagagggccg agcggcggac cagcagcgtc ggcaggatct cctcgacgat cacctcggtc
      361 aggatcgccc ggtcgaaggc ggtggcgatg cgctcggaga cgatgccgat cacctcttcg
      421 gccgtgtggc ggatgccgag gacgtggcgg tccaccaggc tctggaagcg gttgcgcagc
      481 atcgaggcca cggcgacgaa cagcagcgac accagcaggg tggcggtgga gaagcgccgg
      541 atgtccgatt cccacctgct ggagaccacc gacatggcca gcaggtagag ggtgacgtag
      601 gtgaggaaga agctgtaggc ccccagcagc cggttggcgc ggaactcgaa cgaccccagg
      661 tggtgcttgt agatggcgta gacgtagctc atcggcatca tggggatggc gatgagcgcg
      721 atgccgacca tccaggggta gaggctgcgg gcgtccatgg cgaccgccgg gtcgccgagg
      781 attttgggga gggcgatgaa gaacaggatg atgggcccga accccagggc caggccgtag
      841 agcatgatgc gggtggccag ccggacggcg gggtccacgc gcaggaacat gcgcgccagc
      901 agcagcagcg gcgacacgat cgcgccgacc aggaaagagg cgaggtaggc gcgctccagg
      961 cgcggcagga agttgaggtg gtcgaggacc atcagcgccg ccgacaggcc gtacagaggc
     1021 gccagcagcc gcacgcggcg gcggacgaga tagttcggca ggagggtgtg gagatggacg
     1081 gtcagcggca ggaagaacca gatgacggta cggaagacgt atgccgcccc gccggtgtgg
     1141 cggccggagg cgaggccgga ggagaaccag agggcggtga cgtagctgaa gaggaccagg
     1201 gccacccagc gctcgtcccg cggccgcagg aggagcacgg cgacggtgcc catcagccag
     1261 aagacgaggg ggagcaggct ggtgatggtc tcgatgcgcc acagcaggtc gatgccgctg
     1321 aaggcgtagg cctccaccat ccgcacctcg ccgccgcgcc gcacgaggac ctgcgccgtc
     1381 ccgtcccggc cgaacaggtc gagcatgtcg atcgcccagt cgttcttgaa ctcctggagg
     1441 gtcacgccgc ccagctcgag gatctggtcg ccgatctgga cacagtcccg gccggccggg
     1501 cagggatcgg ccacgatcac ccaccagcgg ctgttggctt cgaagctgat cttggggacg
     1561 gtggcgacgt agccgtagta gagacagagg gagaggacca gcagcgagac cgcgctccag
     1621 acgaggtgga ccctcgacga caggaggcgg ccggcggtca tccgccctcg aaggtgagcg
     1681 ccggctccgc catccccacc gactccagga gatgagcgag cggcttcgcc tggatctcca
     1741 gcaggcgctc gatcggcttg cggtcggcga gttggaggcg ggagaggcgc tcgcgcgcgg
     1801 cgtgcgagaa ctcgagcatc cgcagggtgc agtagctcac ggcgccgcag cgcaccagga
     1861 tggtctgcgc cgccgccagg gccgccggct cggcggcgcg ggtcgacagg tggaggaact
     1921 cactgcgctc ggcgtgctcc gccgtcatgg cgtagagcag ggcaaggttg tgggaacggc
     1981 gctgccagtc ggcgccggcc ggcgccttga gcgcgtccga caggtcgtcg ctcacctgga
     2041 tgaaccggcc catgatccgt ccgatctcct cgagcccttc ggcggtctcc gcggtggcgc
     2101 cgccgagcag cgcccccatg tggagcgccg ctccgatgag cggcggcgtc ttggcctcca
     2161 ggacccgcca gtactcctct tcactgctca ggtggcgcgc gtcgagattc tgcccgaaag
     2221 cggtggccag cgccatcccg ccgagcttcg cctgcaacgc cgcccggatc gcgggcgcca
     2281 cgccggggtc gtccagcagc cggtgcgccg cggcctggaa ggccagggcg aggttggccg
     2341 cgatgccgga acccaggcgc cggtagtctc cctgcgggtc ctcgtccagc atgtcgtcca
     2401 ccagatggat gctggtcatc gagcagaaga ccgccgccgc ggccggcgcc gccgcctcct
     2461 cctctcctcc caccgccagg caggcggcca ccgggtactc ccagaccgac cgccactcgc
     2521 ggtggaccgc ccggtccacc aggtcggtca tctggggcca cgcggccact tcggggaggg
     2581 aacgaacccc cgtcttgata cgctcgatcc gcatgggaat tgtctccttt atagcaggtt
     2641 ctgacgccgt ggtccaactt ttgggcgacg gccgcccacg acgcggacga cacggacgac
     2701 gcggtgccgc cgggtggctc ccagcggcac cgtggggaat cagcggtcag cgggacgggt
     2761 cccgcgggct tactcgacgg tccagtcgcc ccaggcgaag tcctgggtgg tgacgaggct
     2821 cttgaggtcg gtggactcgc tcaggcggcg cagggccgcg aagagggcgt tctcgggggt
     2881 ggcggaggca aacttcgagt ggaagtcacg gatcgacatg gtgaaaatct cccgtccagg
     2941 ggactggccc ctgcaaaaaa aggttgaacg acgagctaca ggggaagact ggctgcagga
     3001 ctacagtggg agcgtcagga taggacgcca ggcatgttgt tgttcctcca gaaataaaat
     3061 aaaaggatga cttcaaggtg cggcctgccg gcacacacct cggctcacgc cggtcctata
     3121 gtgtagggac aggccgcagg cgggacaccc cgttttcggt tcgccgcgct cagttgggct
     3181 cgccggacgg ggagccggcg aaccccatgg tggcgaggag ctcgtcgagc ggggtgagga
     3241 agtggtccag cagccgctcc aggggttccg ggtcgtccag ggggatgccg gcgaagaccg
     3301 cccgcgcctg ccgcgagatc tcggccagcc ggaaggcgca gtagctgacc gcgccgcagc
     3361 gcagcaaaat ggcctgcgcc tcggacagcg cccccggctg gtccaggcgc tcgaagagct
     3421 gcagaaagcg gtcgcgctcg gggtgctcca cggtcagggc gtaaagcagc ggcaggttgt
     3481 tgaacggccg gcgccagtcg gggcgggccg gcacctgcag cgcgtccacc aggtcgtcgc
     3541 tgatctgcga gaacagcccc aggtggccgc cgagccgctc gagctgctcg gcggtgacgt
     3601 ccgaggcgcc gcccagcagg gcgccgatcc ccagcgccgc ggcgaacagc ggcggcgcct
     3661 tggtctccac cacccgccag tactcctcct cgttgctcgc cccctgcacg tcgaggctct
     3721 ggccgtaggc ggtggccagg gccatgcgcg cgagccgcga gtggagcgcc gcccgcgtct
     3781 cggggttggg cgacgcgttc tccagcagcc ggtgggccgc cgcctggaag gccagcgcca
     3841 ggttggcggc ggggccttcg ccgacctccc ggtagtcccc ccgcgggtcc tcgtcgagga
     3901 tgtcgtccac cagatggatg ctcagcagcg agcagacgat cgccgccgcc cccggcagcg
     3961 ccgcctcggc gctgcccccc accgcccggc aggccacggc ggggtgctcc cagatcgatt
     4021 gcggctcgtc ccgcaccgca cgctccacca gccgcaccat ctgcggccag gccgacacct
     4081 ccagcaaggc gagaatccgc tgcttcagct catcgagtct catcgacggg ctccggagta
     4141 cgatgggccc tccgggaggg gcagacccct cccggaggcc atccgatgac aggatcgttc
     4201 cggcggcggg cgccggggct tcgctcaggc gtgaccgatg acctggccga cggcggtaga
     4261 gccaccctgc ggcggcgagt cccagctcgg ccagccggtg gcgaccatct ggcggatcaa
     4321 acccccagtg tccccgagcg agctcaggcg gcggagggcg gtgaacagcg gcgtcttctc
     4381 atcggcggag acgaacttgg aatagaactc atgaatggac atatctcatt ccctcgcgtt
     4441 gaaaaaaagc attcaaaggt cgcaacgagc cccggtcctc agtggattcg ccgacgaggt
     4501 ggatatctgg ccatgcaggc ttcccctcct ctctgagccg ccgaactgca ggaggccatt
     4561 tgtaaacaat agtgctttga cggagaggtt ccgtggacaa ccggcgctca atttgagcga
     4621 aaagtgtctt ggccgccctt caggacgggg gtgcggcggt gcgggagagg tcggcgccgc
     4681 cggggcgcgg gtaggcttga ccgccgtgca ggcggccggc gagctcgcgt ccgggcaggg
     4741 gctcggagaa gtagtggccc tggccgtaat cgatgcccag gtggcgcagc acctcggcgt
     4801 cctcggcggt ctcgaccgcc tccatgacgg tggcggtgcc ggcccgggcc gccatctcgg
     4861 cggtgctctg gacgacgatc tgcttgaagg ggtcgcgggc gaggttgcgg cagaaagtgc
     4921 cggagatctt gaggaagtcc ggccgcaggt cctggaacag gttgaggttc gacgagccct
     4981 cgccgaagtc gtccagggcg atgcggaagc cgcgctcgcg cagcttctgg agggtggtgg
     5041 cgaaggcgcg ggggttcagg atagtctgct gctcggtgag ctcgaggacg atgtccttct
     5101 cggccaggcc ggccccgcgc accgcctcgc tcagcttggc ggcgaagacg gggttggtca
     5161 tcgaccgggg ctggacgttc aggaaggtgg cgaccgggat gcccaggtgg cgtacttcgg
     5221 cgagcgccgc ctggatgcag atcatgtcca ccttgaacag cagctccttg tgggcggcgt
     5281 aggccagcaa caccgcgggg ctgccgagga ccgaggcgga ggggccgcgg gccagaccct
     5341 cgacgccgaa cacctcgaac ggctccgccc cctggcgcag gcgcaccagc ggctggagga
     5401 ccgaggcgat ggtgctggtg gcgaggaact cctccagcag ctcgactttc tgcacctcgc
     5461 ccggcggcac cgcggtccgg gcctcgccgc cgccgctctc cgcgggggcc agcgcctcgc
     5521 gccgctccag cagcaggtcg cgcagctccg acagcccgcc gggcttctcc agcacggcgg
     5581 tgacgccgag ggcggcgcag agggcgcgca cccggtcgtc gacgaagccg gagaggacgt
     5641 agacggtgat gtccgggaag tgggtggtga tgaaccggat cagccgcatc ccctcgaggc
     5701 cgccgagccc cgacacggac aggtcggtga ccaccacgtc gaagcggaag cagcggagga
     5761 gcgcctctgc ggcttcgatc tcgaggcagg tggtgaggtg gacccctggc gcgcgcaggc
     5821 acaccgccag caagtcgact accatcgact cgtcgtcgag gactaaaact cggagcacgg
     5881 catgtagtat acgcctgtcc ggggtgatag accccctgaa gatcgatttt cggcccgcct
     5941 acgctccggc gagccgtccc gcccgccgca accgctccag atgggcgagg atctggcgct
     6001 gggcgagccc gtgcgcctcg ggcggtacgt cctcgtacac cgtggggagc atctcccgcg
     6061 gctcgcgccg cccctcgttc caggcggcga ggatcttctc ttcccgccac aggcggtgct
     6121 ggacgtactc gcgcagcttg cccacggcgt cgcggaccac cgggccgtgg gccgggaaca
     6181 gggtgcgcgg cgccagcgcg atcatctttt ccagcgactc caggtagtcg tccatgtccc
     6241 cctcgggcgg gtcgaccacg atcatgccga tgccggccac catgtcgccg gcgagcagcg
     6301 accgctggtc ctcctcgaag aagcagaggt gcccccgcgc gtgccccggc gtatgcacca
     6361 cccgcacggc gcagggggga tcgccggcca gcacgacccg ctggccgtcc acgagctcgc
     6421 cgtcgactcg cacgcggccg gccagccgct cggcggtggc ggggtgggcg aggaccggga
     6481 cgcccagggt gtcgcgcagc cgctccactc cgcccacgtg gtcggggtgg tggtgggtga
     6541 gccagatggc ggtcacccgc cggccgagcc ggtcgcgggc ggcggccagc gcccgctcga
     6601 gccggccgtt ctccacctcc agagtggagc cggggtccac cagcaccgcc tcgccggtgc
     6661 cgaggaggta ggcgttggtg gtggcggccg gcggcagggt gtgggtgagc agcgggaaca
     6721 tcaccacccc ggggcgcatc tccacccggc gcaggggccc gaggttggtc tccgacgggt
     6781 cgcgcagccg ctccaggccg tgcgccgccg ggccgttctc cgccagcacc tcgaggatgt
     6841 ggaggatggg cggggctgcc agcacgtcgc cgctccgcca gcgccgccag gccgcgccgg
     6901 gctcggtcca ctcgccggcc gcgcactcgc cgggcagcac cgagggctgg accgcctcgc
     6961 tgccgggcca ctccagcagg aagaagcggt tgtcgaagcg cagcggggcg aaggggggcg
     7021 tcatccagcg gccggcccac accaggcgcg aggcgtccgg cgtcaggccg aggtccgcca
     7081 tcagctcggc gaacggccgc tcgccggcga gcagggcgcg ccgcgcctcc gccaggtctt
     7141 cagaggatgg ggcgttgccg gagggggtgg cgaggagcag accggtctcc tcgaacagct
     7201 cgcggatggc gcaggccacc aggccgggga cgaggtccgg cccctcggca tccccctgca
     7261 tggactcggg caggccggcg gccggcgcgt ctccggtgcc gcccgcgccc gccacctcgc
     7321 cggccaccgg cactccggcg tcggccctgg agagggcgcc gcccgggaag gcgtgccagc
     7381 cgcccatgaa cggcagggcc tcggcgcgct gaatccagaa cacctcgatc ccgcccgcgt
     7441 cgcggcgccg ccagggcacc accgccgccg aggcgcgggc cggcggcagg ggagagaggg
     7501 ccccgcctct tgccgcggag tcggatattt gaagtaagat ggattcatac agattcttgg
     7561 tggcgtcgct catggccggc ttatatcagg gagcctctcc ctggccaagc cggcgggggc
     7621 ggatccccat aggcgctaac ttaaccctcc aagctgaaaa gcagcctgga cagcttgggg
     7681 ggcctgtttg ctgcgggctc ggggagagtc caagacgtga cgggcgatct cagaggcctt
     7741 gcggatgagc tcggtgaagc tcagc
//
LOCUS       DASPJI010000782         4723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_222270, whole genome shotgun sequence.
ACCESSION   DASPJI010000782 DASPJI010000000
VERSION     DASPJI010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222270"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VEL74_21975"
     CDS             <1..785
                     /locus_tag="VEL74_21975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65265.1"
                     /translation="LLGWSRGGQIGYAYLNNETQIPGGFRNVAGFIPVDIYLKTDDAG
                     LRQLACDRLPLEQAQLAGGTFSSQNGVLISTLGYLALTAPDDLVPPPLNGLFPAGYTN
                     RNAALTVGAATFALANPVPLYHFTGGVFDAEFKPVDLLYTDDLAFLSFETTASPFQPF
                     KELVDADITTCDNPALGDVTWDDNLADIEVPVLYVGAGGGFGEYGIYTTTLLGSTDVT
                     THVVSLVPPAARLFDFGHADLFLAGDAPALVWQPILDWLQAH"
     gene            875..1735
                     /locus_tag="VEL74_21980"
     CDS             875..1735
                     /locus_tag="VEL74_21980"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HYG65266.1"
                     /translation="MPSLSEPNRPLAALLWTGLLLAAGEAVAAPAFQMFPASNLYPGY
                     VADPRRPQFSVALLNFPDPQVEDSGDRRVGLKLGGRFGLLRLHSSERPERGVQLDIEA
                     GFTGQFDIEHSLDNLGWDGTYGLLLSSTFGNGLSVQLGTKHISSHVGDEYAERTGRRR
                     IDYTREELAAGGAWSLGPRWRTYAEYGWGYQPKEEIGQEPGRLQLGLEHEAAGSLGGR
                     LGWYAALDLGAFEERDWQVDPTLQTGLLIPSGDRQWRIGIAYHDGIVPIGELFREDEA
                     YVALGVWLDP"
     gene            2015..3544
                     /locus_tag="VEL74_21985"
     CDS             2015..3544
                     /locus_tag="VEL74_21985"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYG65267.1"
                     /translation="MKNFTDFGLHSDLLRGVADLGFTEPTPIQADAIPPAMTGRDLLA
                     CAMTGSGKTAAFLLPILQQLIDRPARKKTRVLILSPTRELAAQTAEHLEALARHTPIK
                     GAAIFGGVAMGPQERAFRQGVDVLVATPGRLLDHLQNPYASLGDIEILVLDEADRMLD
                     MGFLPDIKRVLAQLPAERQNLLFSATMPPPIAALAREILKNPASLHVERKAAPASGIT
                     QAVYPVPEDLKPTLLVELLRRGDIKNVLVFTRTKHRANRLAEWLERHGVSCDRIHGNR
                     SQSQRTDALARFKNGDLQVLVATDIAARGIDVEALSHVINFDVPNVPEDYIHRVGRTA
                     RAGAVGDAFTFCAPQEQADLNAIERAVGRRLDRRTVPGFDYQAKPAERFEVPIGERIA
                     AIRARKAEERARAKAKAERKAQRESEEKGRTERRSATPAPRQPAAPGHRPANAASAGH
                     HRPGGARKKGQHQPVGVMQQATQRKPAGSPHTPEARAEVTRAFTPSALRNRGGDRQGG
                     R"
     gene            3667..3987
                     /locus_tag="VEL74_21990"
     CDS             3667..3987
                     /locus_tag="VEL74_21990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HYG65268.1"
                     /translation="MGRFDFETERDEGVATEERQEPKKAKRFKVVLHNDDYTTMEFVV
                     HILMTHFHKAPAEAFHIMLQVHHEGVGVAGVYPREVAETKVAEVTDEARANGMPLLVT
                     AEAE"
     gene            4004..>4723
                     /locus_tag="VEL74_21995"
     CDS             4004..>4723
                     /locus_tag="VEL74_21995"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HYG65269.1"
                     /translation="MKLSQDLQISLSVAMTEAQRLGHEYTGLDHLLYALTFDAKTATV
                     MRHAGANVERLQKRLASYLADEVESVRFDDDEEEDFEPRLTLGLQRALSRAAAQVESA
                     GKPEVQGPDLLVAMFAEADSYGMELLAADGVTRLDVVSFIAHGISKLQSSVRALPSGR
                     PDSSTSEDRAPAGDDEDERGFGAVPGGDALASFTQELTEMARKGAIDPLIGRDNEVRR
                     TLHILQRRRKNNPIYVGDPGVG"
BASE COUNT          734 a         1807 c         1517 g          665 t
ORIGIN      
        1 acctcctcgg ctggagccgg ggcggccaga tcggctatgc ctacctgaac aacgagacgc
       61 agatccccgg cgggttccgc aacgtggcgg gcttcatccc ggtggacatc tacctcaaga
      121 cggacgacgc ggggctgcgc cagctcgcct gcgaccggct gcccctggag caggcccaac
      181 tcgccggcgg caccttctcc agccagaacg gcgtgctgat ctcgaccctc ggttacctcg
      241 cgctgacggc gcccgacgac ctggtgccgc cgccgctgaa cggtctcttc ccggccggct
      301 atacgaaccg gaacgccgcc ctgacggtcg gcgccgccac ctttgccctg gcgaacccgg
      361 tgccgctcta ccacttcacg ggtggcgtct tcgacgccga gttcaagccg gtcgacctgc
      421 tctacacgga cgacctggcc ttcctgagct tcgagaccac ggcgtcgccc ttccagccct
      481 tcaaggagct ggtcgacgcg gacatcacca cctgcgacaa cccggccctg ggtgacgtta
      541 cctgggacga caacctggcc gacatcgagg tgccggtcct ctacgtgggc gccggcggcg
      601 gcttcgggga gtacgggatc tacaccacga ccctcctcgg cagcaccgac gtcaccaccc
      661 acgtggtgag cctggtgccg cccgcggcgc gcctcttcga cttcggccac gccgacctct
      721 tcctggccgg cgacgccccg gccctcgtct ggcagccgat cctcgactgg ctccaggccc
      781 actgagcagc aacctccatg caggggcggg ggaaatcccc gccccgcctt ttttccccat
      841 cccgctccga gggctcctcc gttaggatct gcccatgccc tccctctccg agccgaaccg
      901 ccccctcgcc gccctcctct ggaccggcct cctcctggcc gccggcgagg cggtcgccgc
      961 ccccgcgttc cagatgttcc ccgcttcgaa cctctacccc ggatacgtgg cggacccccg
     1021 gcggccgcag ttcagcgtcg cgctgctcaa ctttcccgat ccacaggtgg aggactcggg
     1081 ggaccggcgc gtcggcctca agctcggggg acggttcggg ctgctgcgcc tccactcctc
     1141 cgaacggccg gagcgcggcg tgcaactgga catcgaggcc ggcttcacgg gccagttcga
     1201 tattgagcac tcgctcgaca acctggggtg ggacggcacc tacggattac tcctcagctc
     1261 caccttcggc aacggcctca gcgtgcagct cggcaccaag cacatctcgt cccacgtggg
     1321 ggacgagtac gccgagcgca ccggacggcg gcgcatcgac tatacgcggg aggagctggc
     1381 ggccggcggc gcgtggtccc tcggcccccg ctggcgcacc tacgccgagt acggttgggg
     1441 gtaccagccc aaggaggaga tcggccagga gcccggacgg ctgcagctcg gcctcgagca
     1501 cgaggcggcc ggcagcctcg gggggcgcct ggggtggtac gccgccctcg acctcggcgc
     1561 cttcgaggag cgggactggc aggtggaccc gacgctccag accgggctcc tgatcccctc
     1621 cggcgaccgc cagtggcgga tcgggatcgc ctatcacgac ggcatcgttc ccatcggcga
     1681 gctcttcaga gaggacgagg cctacgtcgc cctcggggtc tggctggatc cttgacctct
     1741 ggaggcagcc tctggaatgc ctgcgctcca aggggtgttc tagggtaggt atccgcagcc
     1801 cgggttggca aaaccggtct tggtgtgaga tcgtctcgac tccaccgcgg tctggcccct
     1861 aagtccgggt cagccttcgc ggaccatcga acaggggctc cgagccctga gcggcgacct
     1921 accggcaagt tcccgttcct cctttaccgc cggcttgact cgaagggtgc agtccctctc
     1981 ctggaccggt gcgccctcag gaagactcta gaacttgaag aatttcactg atttcggcct
     2041 ccactccgac ctcctgcgcg gcgtcgcgga cctcggcttc actgaaccga cccccatcca
     2101 ggcggacgcc atcccgccgg ccatgaccgg acgcgacctg ctcgcctgcg ccatgacggg
     2161 cagcggcaag accgcggcgt tcctgctgcc gatcctgcag cagctcatcg atcggcccgc
     2221 ccgcaagaag acccgggtgc tgatcctgag ccccacccgg gagctggcgg cgcagaccgc
     2281 cgagcacctg gaggcgctcg cccgccacac cccgatcaag ggcgcggcga tcttcggcgg
     2341 cgtcgccatg gggccgcagg agcgcgcctt caggcagggc gtcgacgtcc tcgtcgccac
     2401 ccccggcagg ctcctcgacc acctgcagaa cccctacgcc agcctcggcg acatcgagat
     2461 cctcgtcctc gacgaggccg accgcatgct ggacatgggc ttcctgccgg acatcaagcg
     2521 ggtcctggcg cagctcccgg ccgagcggca gaacctcctg ttctccgcca ccatgcctcc
     2581 gcccatcgcg gccctggcgc gggagatcct gaagaacccc gccagcctgc acgtggagcg
     2641 caaggcggct ccggcgtcgg gcatcaccca ggccgtctac ccggtgccgg aggacctgaa
     2701 gcccaccctg ctggtggagc tgctgcggcg cggcgacatc aagaacgttt tggtgttcac
     2761 ccgcaccaag caccgggcca accgtctggc ggagtggctg gagcgccacg gcgtctcctg
     2821 cgaccgcatc cacggcaacc gcagccagtc gcagcgcacc gacgccctgg cgcggttcaa
     2881 gaacggcgac ctccaggtgc tggtggccac cgacatcgcg gcccgcggca tcgacgtcga
     2941 ggcgctgtcg cacgtcatca acttcgacgt gcccaacgtg cccgaggact acatccaccg
     3001 ggtgggccgg acggcgcgcg ctggcgcggt gggcgacgcc ttcaccttct gcgcgccgca
     3061 ggagcaggcc gacctgaacg ccatcgagcg ggcggtgggc cgccgtctgg atcgccgcac
     3121 agtgccgggc ttcgactacc aggccaagcc cgcggagcgc ttcgaggtcc cgatcgggga
     3181 gcgcatcgcc gccatccgcg cccgcaaggc cgaggagcgc gcccgcgcca aggccaaggc
     3241 ggagcgcaag gcgcagcgcg agtccgaaga gaaggggcgc acggagcgcc ggtcagccac
     3301 cccggccccg cgccagccgg ccgcccccgg tcaccggccc gccaacgcgg cctccgccgg
     3361 gcaccaccgg cccggcggtg cccgcaagaa gggccagcac cagccggtgg gcgtcatgca
     3421 gcaggcgacg caacgcaagc cggcggggag cccgcacacc cccgaggccc gggcggaggt
     3481 gacccgcgcg ttcaccccct cggccctccg caaccgcggc ggcgaccgcc agggcggccg
     3541 ctaggcctcg cctgctcccg tcccctcccg ccagggcgag gggacgggaa tcctcccctc
     3601 caatccccgc gttgatcccc acgggtatac tcctctccgg gagtccttcc ccccgcgtga
     3661 ggagtcatgg ggcgcttcga tttcgagaca gaacgcgacg aaggggtcgc caccgaggag
     3721 cggcaggagc cgaagaaggc caagcgcttc aaggtggtgc tccacaacga cgactacacg
     3781 acgatggagt tcgtcgtgca cattctcatg acgcacttcc acaaggctcc cgctgaggcc
     3841 ttccacatca tgctccaggt ccaccacgag ggcgtgggcg tggccggcgt ctatccgcgg
     3901 gaggtggccg agaccaaggt ggccgaggtc accgacgagg cgcgtgccaa tggcatgcct
     3961 ctcctggtga ccgccgaggc cgaatgacag gattcgcaga cccatgaagc tctctcagga
     4021 tctccagatc tccctgagcg tggcgatgac cgaggcgcag cgcctgggcc acgagtacac
     4081 cgggctcgat catctcctct acgctctcac cttcgacgcc aagaccgcca ccgtcatgcg
     4141 ccacgccggc gccaacgtcg agcggttgca gaaacggctc gcctcctacc tcgccgacga
     4201 ggtcgagagc gtgcgcttcg acgatgacga ggaagaggac ttcgagcccc gcctcaccct
     4261 cggcctgcag cgcgctctct cccgcgccgc cgcgcaggtg gagagcgccg gcaaaccgga
     4321 ggtccagggg cccgacctcc tggtcgccat gttcgccgag gccgactcct acggcatgga
     4381 gctcctcgcc gccgacggcg tcacccggct cgacgtcgtg agcttcatcg cccacggcat
     4441 ctccaagctc cagtcgtccg tgcgcgcttt gcccagcggc cggcccgact cctccacctc
     4501 cgaggaccgc gcccccgccg gcgacgacga ggacgagcgc ggcttcggcg cggtgcccgg
     4561 cggcgacgcc ctggcctcgt tcacccagga gctgaccgag atggcgcgca agggggcgat
     4621 cgaccccctg atcggccgcg acaacgaggt ccggcgcacg ctccacatcc tgcagcgccg
     4681 gcgcaagaac aaccccatct acgtgggcga ccccggcgtc ggc
//
LOCUS       DASPJI010000783        11069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_728382, whole genome shotgun sequence.
ACCESSION   DASPJI010000783 DASPJI010000000
VERSION     DASPJI010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11069)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11069
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728382"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="VEL74_22000"
     CDS             <1..1353
                     /locus_tag="VEL74_22000"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HYG65270.1"
                     /translation="VGPMQRESLWHLSEWFTGTGENFRAIREYNELSDDDVQRGKSVV
                     IPAELLRPAFRSVLPVPETPFDLDYGKDKDGEYAIYRLRPGEALYSSVVVRFTGRLHG
                     PDVNALAADIARRSGVADVTDIPIGYRIKIPFDVLQPEFMPEGHPQRLEYEAELRASA
                     RYSNQVKARGLAGITVILDAGHGGQDSGAAMGGVWESLYVYDIAMRTKALLESTTAAR
                     VHVTTRDGKDHRVADSDVLPFSRGHSVLTTPPYPITDATVGVNLRWYLANSVYRQAVD
                     KGGDPQKVVFISIHADSLHPSVRGAMVYIPAANMRGGSFGKSGAVYTARKEVQEAQRV
                     TFPWEARVRSEGLSRELAKQLIASFHAGKLPIHPFKPVRDKIIRNKSEWVPAVLRYNT
                     VPAKVLLEVCNLANDQDRKLLQTRAYRQNVAKAIHQGLVAYYGEDDALPTTQIAAGAK
                     "
     gene            1500..2093
                     /locus_tag="VEL74_22005"
     CDS             1500..2093
                     /locus_tag="VEL74_22005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG65271.1"
                     /translation="MGVATISTAAVHEIALDREVIERHRCGDAQAFDEIYQRFAEMVY
                     NLAYRLAGSSEEAADLTQEVFLRVFRHLGSFRGGSTLKTWIFRITINHCRDRLSRWRP
                     LTQPIEDEMVGEVGWSLADPGRGPEELAVAADEGRRVADGLTRLPQPFREAVVLRDIE
                     GLAYDEIAEILQVRVGTVRSRIARGREQLRILLERRV"
     gene            2090..2809
                     /locus_tag="VEL74_22010"
     CDS             2090..2809
                     /locus_tag="VEL74_22010"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYG65272.1"
                     /translation="MSFHIRDEMLSAYLDGEIEPARERTIDHHLNSCEECRARLNGMR
                     GLVLELRRAPRLQPPPPLAHQVRQQVMEQAPAGVWESLRALLAGRPLHPAMHSVMAMG
                     LALVLSVSLVRVEREQRLARTVPPATPTSTEEPVTDVQVLLGRVIYRQTTIQLGDIEF
                     VLYDAQGRFVQEGLEGSEPETRVDSRSPEGRAVLTDRTYLGLVLDEGYSVVLSSKDGR
                     TVEVAPPPLRGASSGWAGVHV"
     gene            complement(2892..4979)
                     /locus_tag="VEL74_22015"
     CDS             complement(2892..4979)
                     /locus_tag="VEL74_22015"
                     /inference="COORDINATES: protein motif:HMM:NF015414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HYG65273.1"
                     /translation="MAISRTTQQHLEKGDFEALESDWLAHLGQDPTDLDYFVGVARAL
                     TGNNEDDRARFLLEMLDEQLRDGRWAVRLKLLQRAGHLLLPPEELHGAIVQSLEKLYG
                     GQSTFKGLAESVGLYRATHDIPKTWEKVERLEGLLAFDIGTVVAMEGKGVGRIVEANL
                     GLESFKVDFEKIRGMTVGFKAAAKLLRPLGPDHVLRRKLENPASLKALVTGNPSELLR
                     LVLQSYDRPLTAGEIRDVVAGVVSESQWTSWWGAARKNPQVVAGGTGRQTYTWAETAG
                     DAHEAVWKTFTKSEPRKQMDLLRREGARNPELRARMAEILSDAAEKAVDGGDPGLAFE
                     IWFALERSGGAPADVDWAPDELLAARAGRDLARLFTGIEERLLRERAYAMVRERRDDW
                     PAIYRDALSRETDPRALDVLAEGLAQGDARELDRFLDGLLAQPHRNPAAFTWLAERAA
                     DDEQIRTRNALRLLQQILTSMARDDFAPYRLRLVALAESGGTLPRLLGHLTEDQAAQA
                     EDAVHRAPNLEGYQRDQLINAIQLRFQGLRKEQGAAALYALAESIDAKHAELHQIMTV
                     EVPANRKAIEEARAMGDLRENFEYKSARQRHEYLNARAAALNAELSRARPIDVTGMDT
                     SEVRIGTRVRLTAPGAPERVMTILGPWESKPEENVISYESDLAKQLLGKKVGSEVEMS
                     GETWTVAGIEAYK"
     gene            5018..5827
                     /locus_tag="VEL74_22020"
     CDS             5018..5827
                     /locus_tag="VEL74_22020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65274.1"
                     /translation="MSEPTPAPEGPPDGGQPASPAAGPPGNQEEGELEVRLREAGADE
                     LLALLEEHAPHLEPPQVRQALRNPFFDARGVEILAEQPRLLSFYEVRRGLALHPRTPE
                     ALALRLVAGLFWRDLLELGADSRLRPVVRRAADLQLAARLPELAIGEKINLARRAGTG
                     LLSHLRHDPSPRVMAALLDNPRMTEGVLAPVVHSDRTSPPVLDLIAGDRRWGVRYGLR
                     QGLCRNPRTPAETVLRLLPSLRKEDLRTVAGDAKLSETVRRRARLLLGEVN"
     gene            5930..6835
                     /locus_tag="VEL74_22025"
     CDS             5930..6835
                     /locus_tag="VEL74_22025"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYG65275.1"
                     /translation="MPPRRNEGNRPADAEPNLAADDAAAGGARARQPPWEKEAEPQAG
                     ASFGTWLRRQREARDVSLRDIADRTKISLRYLEAMEEDRFDLLPAPLFAKGFLREYAR
                     YVGLSPDEVVNHYLSVQGEDKEEEGEQTLIRRRTKASAQKARSLAFYLFFVLAGLALL
                     GLVWFFAYYNDKRQEERPAAAAPPPIAAPPVSAARQAPTPSPPPAEPAAPIEVTLDFT
                     GDCWVEATIDGKRRIAEQRVQGESVELEAEESIALLLGNIRSVTVQVNGQPWDLQPNS
                     PDSLVGNLVVDLDLVRSLREQKEDR"
     gene            6838..7878
                     /locus_tag="VEL74_22030"
     CDS             6838..7878
                     /locus_tag="VEL74_22030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65276.1"
                     /translation="MAGGMAGGGSPLLDQIRSGANRQLQVMAASGLLPLPPEEIIPLQ
                     VELAGGTDFELALRAKESLRSVDPRVAVPYLAKTAGEEVLFFFALEATQHPVIEALLR
                     RRDVPRQALEVLAKRVPPDLQEILILRQDAIVESPAILDALEENPELSAYTQRRISEY
                     RQHLLPRERTPAGPAPVHAPGEIEECDDATLAREIAAVRSKPVDGEVEERTGLSEGQI
                     RMLPVPARLKLARGAPRLLRSMLMRDSNVQVALAVLRHNTLSDQEVEMVASSRAVVED
                     VLIEVSKRREWVGKYNIAKALVQNPRCPLPTALRLLSRMSVRDLREIARDRNVPDAVR
                     STALRLYTIKQK"
     gene            7887..8522
                     /locus_tag="VEL74_22035"
     CDS             7887..8522
                     /locus_tag="VEL74_22035"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HYG65277.1"
                     /translation="MARDFYVTLALPRNATREQIRQRFRELARERHPDRFPGAAKARA
                     EAEFQAITEAFNVLHDPDRRRQHDLELARPTDANDPRQVARLYMQRGVKSYKEKSFLD
                     AADNFDRATKADPNNAQAWHHLAQASSQQESWLPRAVAAIERACELEPMNPSYLKQAG
                     RILALAGQADKARDFYRRALQWGEDDPAVHRALEELNGPAPRRGGLFGRAG"
     gene            8522..9334
                     /locus_tag="VEL74_22040"
     CDS             8522..9334
                     /locus_tag="VEL74_22040"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HYG65278.1"
                     /translation="MLRVKACGLSDIGLTRLHNEDYFEIDPVHKLYVVADGMGGHNHG
                     EVAAQIAVNSIREFVAKSSDQDTTWPFGIDNRLARHSNLLKMAVRMAHDHVLRAIRRD
                     GSLYGMGTTVVGMIFDGARAAVAHVGDSRAYRLRGNRLDQLTQDHTWVNEQVVAGFLS
                     KEQARSHPLKNVVTRALGGESDVLVDVREVEVQPGDIYLLCSDGLTGMLSDADIRDRL
                     LSGRSLHEICRSLINDSNARGGIDNTTVVLLAVDDAEDADEIETDELGAMTG"
     gene            9335..>11069
                     /locus_tag="VEL74_22045"
     CDS             9335..>11069
                     /locus_tag="VEL74_22045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65279.1"
                     /translation="MLVYGDAKRVEAVAAKVERLLGLLSRTVFLDPGIERHAAFAALL
                     VEAGELEQGLLDEQLQRSGEERPTPLSEAAGRLTRAAADLFESSYRSCGSRPAEIVEG
                     TAPVVLALEEILELPLSGEVSVTVPEGYAFYGLYPETYLEAARRAVELEEGPVQVIGI
                     RSIGTSLAAAVAAGVGDGATTVTVRPGGHPFSRTLAMGEEMERRLLSRPGEVRFAVAD
                     EGPGLSGSSFGSVADWLEERGVLPDSISFFPSHRGGPGPYASERHRERWERARRHTVD
                     FEELFVAPGASWPLASWVADLTGSAEGPLEDVSAGRWRQRLFPGPGSRPPAHLQQERR
                     KYLLTAGGRKWLLKFVGLGRYGREALDLARTLESKGLVPPVAGFRHGFLVGPWLADED
                     GARPLPLVPDLDRAALLEQVATYLAFRAARLPAGPERRGAAPGKLLEMARFNTGKALG
                     PEAAERLDAWEERLPDLAARERPILSDNRLHAWEWLVLPGGRILKADALDHHRSNDLV
                     GEQDVAWDQAGAAIELNLSEEEGERLREEVARRSGVVSTTDQVRFYTLTYLAFQLGAC
                     RLAAEALEGMDG"
BASE COUNT         1602 a         3906 c         4011 g         1550 t
ORIGIN      
        1 gtcgggccga tgcagcgcga gagcctctgg cacctctccg agtggttcac cggcacgggg
       61 gagaacttcc gggcgatccg cgagtacaac gagctgtccg acgacgacgt gcagcgcggg
      121 aagtccgtgg tcatcccggc ggagctgctg cgtccggcct tccggtcggt cctgccggtg
      181 ccggagaccc ctttcgacct cgactacggc aaggacaagg acggcgagta cgccatctat
      241 cggctgcgtc ccggcgaggc gctctactcg agcgtcgtcg tccgcttcac cggccgcctg
      301 cacggcccgg acgtcaacgc gctcgccgcc gacatcgccc ggcgcagcgg cgtcgcggac
      361 gtcaccgaca tcccgatcgg ctaccggatc aagatcccgt tcgacgtgct gcagcccgag
      421 ttcatgcccg aggggcatcc gcagcgcctc gagtacgagg ccgagctgcg ggccagcgcg
      481 cgctacagca accaggtcaa ggcgcggggg ctcgccggca tcaccgtgat cctcgacgcc
      541 ggccacggcg gccaggactc gggcgccgcc atggggggcg tctgggagag cctgtacgtc
      601 tacgacatcg cgatgcgcac caaggcgctg ctcgagtcga cgaccgccgc ccgggtgcac
      661 gtcaccaccc gtgacggcaa ggaccaccgg gtggccgact ccgacgtgct gccgttctcg
      721 cgcggccact cggtgctgac cacgccgccg tacccgatca ccgacgcgac ggtgggggtc
      781 aacctgcgct ggtatctggc gaacagcgtc taccggcagg cggtggacaa ggggggggac
      841 ccgcagaagg tggtgttcat ctccatccac gccgactccc tccacccgtc ggtgcgcggc
      901 gccatggtct acatcccggc ggccaacatg cgcggcggca gcttcggcaa gagcggcgcg
      961 gtctacacgg cgcgcaagga ggtgcaggag gcgcagcggg tgaccttccc ctgggaggcg
     1021 cgggtgcgca gcgagggcct gtcgcgcgag ctcgccaagc agctcatcgc ctccttccac
     1081 gccggcaagc tgccgatcca cccgttcaag ccggtgcgcg acaagatcat ccgcaacaag
     1141 agcgagtggg tgccggcggt gctgcgctac aacaccgtgc cggccaaggt gctgctggag
     1201 gtctgcaacc tggcgaacga ccaggaccgc aagctcctcc agacccgcgc ctatcgccag
     1261 aacgtggcca aggccatcca ccaggggctc gtcgcctact acggcgagga cgacgccctg
     1321 cccaccaccc agatcgcggc cggggcgaag tgaccccgct ccggaaggcc ccctggggag
     1381 tacaatccgg cgaaaccgtt cccgaagcag gccgccgcca ggtccgcggg aacttttttc
     1441 agccagggcc cgtctcttat aggtgaaggg tgcgatgctc gcacccgagg gagggtccgg
     1501 tgggagtagc cacgatttcc actgctgccg tacacgagat cgctctcgac cgcgaagtca
     1561 tcgagcgtca ccgctgtggc gacgcgcaag ccttcgacga gatctaccag cgcttcgcgg
     1621 agatggtcta caacctggcc taccgtctgg ccgggagcag cgaggaggcg gcggacctca
     1681 cccaggaggt gttcctccgg gtgttccgcc atctcggcag cttccggggt ggctccaccc
     1741 tcaagacctg gatcttccgg atcaccatca atcattgccg cgaccggctt tcgcgctggc
     1801 gcccgctgac ccagcccatc gaagacgaga tggtgggaga ggtggggtgg agcctcgccg
     1861 atcccggccg cggtcccgag gagctggcgg tcgccgccga cgaggggcgc cgggtggccg
     1921 atgggctcac ccgcctgccg caaccgttcc gggaagcagt ggtcctgcgc gacatcgagg
     1981 ggttggctta cgacgagatc gcagagatcc tgcaggtccg ggtcggcacg gtgcgctcgc
     2041 ggatcgcgcg cggccgtgag cagctccgta tcctgttgga gaggagagta tgagcttcca
     2101 cattcgcgac gagatgctct cggcgtatct cgatggggag atcgagcccg cccgagagcg
     2161 aaccatcgac caccatctca actcctgcga ggagtgccgc gcccggctca acggcatgcg
     2221 cggcctggtc ctggagctgc ggcgcgcgcc gcgcctccag ccgccgcccc ccctcgccca
     2281 ccaggtccgc cagcaggtca tggagcaggc gccggccgga gtctgggaga gcctgcgcgc
     2341 cctgctcgcc ggccggccgc tccatccggc catgcacagc gtgatggcca tggggctcgc
     2401 cctggtgctg agcgtctcgc tggtccgcgt cgagcgtgag cagcggctgg cgcggaccgt
     2461 gccgccggcg acgccgacga gcaccgagga gccggtcacc gacgtccagg tgctcctggg
     2521 cagggtgata tacaggcaga ccaccatcca gttgggggac atcgagttcg tcttgtacga
     2581 cgctcagggg cgttttgtgc aggaggggct ggaagggagc gagcccgaga cccgggtcga
     2641 cagccgcagc ccggagggcc gggcggtgct caccgaccgg acctacctcg gtctggtgct
     2701 cgatgagggc tacagcgtgg tgctgagctc caaggacggc cggacggtgg aggtcgcgcc
     2761 gccgccgctg cggggggcca gctccggctg ggccggcgtc cacgtctgag ccacggcaac
     2821 gagccgtacc ctgcaggccc ggcggcgcca gccgccgggc ttcgctgttt tcagggggct
     2881 actaccaacg actacttgta ggcctcgatc cccgccaccg tccaggtctc gccgctcatc
     2941 tccacctcgg agcccacctt cttgccgagg agctgcttcg cgaggtccga ctcgtaggag
     3001 atgacgttct cctcgggctt gctctcccag gggccgagga tggtcatcac ccgctcgggc
     3061 gcgccgggag cggtgaggcg gacgcgagtg ccgatgcgca cctcggaggt gtccatgccg
     3121 gtgacgtcga tggggcgggc gcgcgacagc tcggcgttca gggcggcggc gcgggcgttc
     3181 aggtactcgt ggcgctggcg ggccgacttg tactcgaagt tctcgcgcag gtcgcccatg
     3241 gcccgggcct cttcgatcgc cttgcggttg gccggcacct ccaccgtcat gatctggtgc
     3301 agctcggcgt gcttggcgtc gatcgactcc gccagggcat agagcgcggc tgccccctgc
     3361 tccttgcgca gcccctggaa gcgcaactgg atggcgttga tgagctggtc ccgctgatag
     3421 ccctcgaggt tcggggcgcg gtgcacggcg tcctcggcct gggcggcctg atcctcggtg
     3481 aggtggccga gcaggcgcgg cagcgtgccg ccggactccg ccagcgccac gaggcgcagg
     3541 cggtaggggg cgaaatcgtc gcgcgccatg ctggtgagga tctgctgcag caggcgcagc
     3601 gcgttgcggg tgcggatctg ctcgtcgtcg gcggcgcgct cggcgagcca ggtgaaggcc
     3661 gccgggttgc ggtgcggctg ggcgaggagg ccgtcgagga agcggtccag ctccctggcg
     3721 tccccctggg ccagcccctc ggcgagcaca tccagggcgc gcgggtcggt ctcccggctg
     3781 agggcgtcgc ggtagatggc aggccagtcg tcccggcgct cgcgcaccat ggcataggcc
     3841 cgctcgcgca gcagccgctc ctcgatgccc gtgaagaggc gggcgaggtc gcggccggcg
     3901 cgggcggcga gcagctcgtc gggcgcccag tcgacgtccg cgggggcgcc gccgctgcgt
     3961 tccagggcga accagatctc gaaggcgagg ccggggtcgc cgccgtcgac cgccttctcg
     4021 gcggcgtcgg agaggatctc cgccatgcgc gcgcgcagct cggggttgcg cgccccttcg
     4081 cggcgcagca ggtccatctg cttgcgcggc tcggacttgg tgaaggtctt ccacaccgcc
     4141 tcgtgggcgt cgccggcggt ctcggcccag gtgtaggtct ggcggccggt gccgcccgcc
     4201 accacctgcg ggttcttgcg cgccgcaccc caccaggagg tccactgcga ctcgctgacg
     4261 acgccggcca ccacgtcgcg gatctcgccc gcggtgagcg gccggtcgta gctctggagg
     4321 accaggcgca gcagctccga cgggttgccg gtgaccagcg ccttgagcga cgcggggttc
     4381 tccagcttgc ggcgcagcac gtgatcgggg ccgagggggc gcagcagctt ggccgccgcc
     4441 ttgaagccga cggtcatgcc gcggatcttc tcgaagtcca ccttgaagct ctcgagcccc
     4501 aggttggcct cgacgatccg gccgacaccc ttgccctcca tggcgacgac ggtaccgatg
     4561 tcgaaggcga ggagcccttc gagccgctcc accttctccc aggtcttggg gatgtcgtgc
     4621 gtcgcccggt acaggccgac ggactcggcg agccccttga aggtgctctg gccgccgtag
     4681 agcttctcca ggctctggac gatggcgccg tgcagctcct cgggggggag gagcaggtgg
     4741 ccggcgcgct gcagcagctt gaggcggacg gcccagcggc cgtcgcggag ctgctcgtcg
     4801 agcatctcga gcaggaagcg ggcgcggtcg tcctcgttgt tgccggtcag ggcgcgggcg
     4861 acgcccacga aatagtcgag gtctgtgggg tcctggccga ggtgggccag ccaatcgctc
     4921 tcgagcgcct cgaagtcccc cttctcgagg tgctgctggg tggtacggga aattgccatc
     4981 gggatcagga agataccaga ttctgtaagc taccctcatg tcggagccga ccccagcccc
     5041 cgaaggcccc cccgatgggg gccagccggc gagcccggcg gcgggcccgc cggggaacca
     5101 ggaggagggc gagctcgagg tccgcctgcg cgaggccggg gccgacgagc tgctcgccct
     5161 gctggaggag cacgcgccgc acctcgagcc gccgcaggtg cggcaggcgt tgcgcaaccc
     5221 gttcttcgac gcccgcggcg tggagatcct ggcggagcag ccgcggctcc tctccttcta
     5281 tgaggtgcgg cggggcctcg cccttcaccc gcgcacgccc gaggcgctgg ccctgcggct
     5341 ggtcgccggg ctcttctggc gcgacctgct ggagctcggc gccgacagcc ggctgcgccc
     5401 agtggtgcgg cgggccgccg acctgcaact ggccgcgcgg ttgcctgagc tggcgatcgg
     5461 agagaagatc aacctggcgc ggcgtgccgg cacgggcctt ttgagccatc tgcggcacga
     5521 tccgagccca cgggtgatgg cggcgctgct cgacaacccg cggatgacgg agggggtcct
     5581 ggcgccggtg gtgcacagcg accggacctc gccgccggtc ctcgacctga tcgcgggtga
     5641 ccggcgctgg ggagtgcgct atggtctgcg gcagggcctc tgccgcaatc cgcggacgcc
     5701 ggcggagacc gtgctccggc tcctgccgtc gttgcggaaa gaggacctgc ggaccgtggc
     5761 cggcgacgcg aagctgtccg agacggtgcg ccgccgggcg cggctcttgc tgggagaagt
     5821 gaattgaaga ggtatttcgg ggcgcggcgg atcgacctca ttgaccagcg tgcagcagcc
     5881 tcgtaaagtt atgactcgcg taccgaacga catgtcgaaa cgacaacgga tgccaccccg
     5941 cagaaacgag ggcaacaggc ccgccgacgc cgagccgaac ctcgccgccg acgacgccgc
     6001 tgccgggggc gcgcgcgcgc gccagccgcc ctgggagaag gaggccgagc cgcaggccgg
     6061 cgcctccttc gggacctggc tccggcgcca gcgggaggcg cgggacgtca gcctgcgtga
     6121 catcgccgac cgtaccaaga tcagcctgcg ctatctggag gcgatggagg aggaccgctt
     6181 cgacctcctg ccggcgccgc tgttcgccaa gggcttcctg cgcgaatatg cccgctacgt
     6241 cgggctgtcg ccggacgagg tggtgaacca ctacctgtcg gtccagggcg aggacaagga
     6301 ggaggagggg gagcagacgc tgatccgccg gcgcaccaag gcgtcggccc agaaggcgcg
     6361 gagcctggcg ttctatctgt tcttcgtcct cgccggcctg gcgctgctgg gcctggtctg
     6421 gttcttcgcc tactacaacg acaagcggca ggaggagcgg cccgccgccg cggcgccgcc
     6481 gcccatcgcc gcgccgccgg tttcggcggc ccggcaggcc ccgaccccct cgccgccgcc
     6541 cgccgagccg gcggcgccga tcgaggtcac cctcgacttc accggtgact gctgggtgga
     6601 ggcgaccatc gacggcaagc gccggatcgc cgagcagcgg gtccagggcg agtcggtgga
     6661 gctcgaggcc gaggagagca tcgccctgct cctcggcaac atccggtcgg tcaccgtcca
     6721 ggtcaacggc cagccctggg acctccagcc caactcgccc gacagcctcg tcggcaatct
     6781 ggtggtcgac ctcgatctgg tgcgcagcct tcgcgagcag aaagaggacc gctgaacgtg
     6841 gccgggggta tggccggcgg gggctcgccc ctcctcgacc agatccggtc gggagccaac
     6901 cggcagctcc aggtgatggc ggccagcggc ctcctccccc tgccgccgga ggagatcatc
     6961 cccctgcagg tcgagctggc cggcggcacc gacttcgagc tggcgttgcg ggccaaggag
     7021 agcctccgct cggtggaccc gcgggtggcg gtgccctacc tcgccaagac cgccggcgag
     7081 gaggtgctct tcttcttcgc cctggaggcc acccagcacc cggtcatcga agcgctgctg
     7141 cgccgccgcg acgtgccgcg ccaggcgctg gaggtgctgg cgaagcgggt gccgccggac
     7201 ctccaggaga tcctcatcct gcgccaggac gcgatcgtcg agtcgccggc gatcctcgac
     7261 gcgctggagg agaatccgga gctcagcgcc tacacccagc ggcggatcag cgagtaccgg
     7321 cagcatctgc tgccgcggga gcggacgccg gcgggcccgg ccccggtgca cgcgcccggc
     7381 gagatcgagg agtgtgatga cgcgaccctc gcccgggaga tcgccgcggt gcgctcaaag
     7441 ccggtggacg gcgaggtgga ggagcggacc ggcctctccg aggggcagat ccgcatgctc
     7501 cccgtccccg cccgcctgaa gctcgcccgc ggcgcgccgc gcctgctgcg cagcatgctg
     7561 atgcgggact ccaacgtcca ggtcgccctc gccgtgctgc gccacaacac cctgtccgac
     7621 caggaggtcg agatggtggc gagcagccgg gcggtggtgg aggacgtgct gatcgaggtc
     7681 tcgaagcggc gcgagtgggt gggcaagtac aacatcgcca aggcgctggt gcagaacccc
     7741 cgctgccctc tgcccacggc cctgcgcctg ctctcccgga tgtccgtccg ggacctgcgg
     7801 gagatcgccc gcgaccgcaa cgttcccgac gccgtgcgct cgacagccct ccgtttgtat
     7861 acaatcaagc agaaataggc gtcgacatgg ccagggattt ctacgtcacc ctcgcgttgc
     7921 cgcgcaacgc gacgcgcgag cagatccgcc agcgcttccg ggagctcgcg cgcgagcgcc
     7981 atccggaccg cttcccgggg gcggccaagg cgcgcgccga ggcggagttc caggcgatca
     8041 cggaagcctt caacgtcctt cacgacccgg accggcggcg ccagcacgac ctggagctgg
     8101 cgcgcccgac ggacgcgaac gacccccggc aggtcgcccg cctgtacatg cagcgcggcg
     8161 tcaagtcgta caaggagaag agcttcctcg acgcggcgga caacttcgac cgcgccacca
     8221 aggcggaccc gaacaacgcc caggcgtggc accatctggc ccaggcctcc agccagcagg
     8281 agagctggct gccgcgggcg gtggcggcca tcgagcgggc ctgcgagctg gagccgatga
     8341 acccctccta tctcaagcag gcgggccgca tcctggcgct ggccggccag gccgacaagg
     8401 cccgcgactt ctaccgccgg gccctccagt ggggcgagga cgaccccgcg gtccaccggg
     8461 ccctggagga gctgaacggc cccgctcccc gccgcggcgg cctgttcgga agggccggct
     8521 gatgttgcgc gtcaaggcct gcggcctctc ggacatcggg ctgacccggc tccacaacga
     8581 ggactacttc gagatcgatc cggtccacaa gctgtacgtg gtggccgacg gcatgggggg
     8641 ccacaaccac ggcgaggtgg cggcgcagat cgcggtcaac tcgatccgcg agttcgtcgc
     8701 caagtcctcg gaccaggaca ccacctggcc gttcggcatc gacaaccgcc tggcgcgcca
     8761 ctcgaacctg ctcaagatgg cggtccgcat ggcccacgac cacgtgctgc gcgccatccg
     8821 ccgcgacggc tcgctctacg gcatggggac cacggtggtg gggatgatct tcgacggcgc
     8881 gcgcgcggcg gtggcgcacg tgggggacag ccgcgcctac cgtctgcgcg gcaaccggct
     8941 ggaccagctc acccaggatc acacctgggt caacgagcag gtggtggccg gtttcctgtc
     9001 caaggagcag gcgcgctcgc acccgttgaa gaacgtggtc acccgcgccc tgggcggcga
     9061 gagcgacgtg ctggtggacg tgcgcgaggt cgaggtccag ccgggggaca tctatctcct
     9121 ctgctcggac ggcctgaccg gcatgctgag cgacgcggac atccgcgacc gcctgctctc
     9181 gggccgcagc ctgcacgaga tctgccgctc cctgatcaac gactccaacg cccgcggcgg
     9241 catcgacaac accaccgtgg tgctgctggc ggtcgatgac gctgaagacg ccgacgagat
     9301 cgagaccgac gagctcgggg cgatgaccgg ctaggtgctc gtctacggcg acgccaagcg
     9361 ggtggaggcc gtggcggcca aggtggagcg cctgctcggg ctcctgtcgc gcaccgtctt
     9421 cctggacccc gggatcgagc ggcacgccgc cttcgccgcg ctgctcgtcg aagcggggga
     9481 gctggagcag ggcctcctcg acgagcagct ccagcgttcc ggtgaagagc ggcccacgcc
     9541 gctgagcgag gcggcggggc ggctgacgcg ggcggcggcc gacctgttcg agagctcgta
     9601 ccggagctgc ggctcccggc ccgcggagat cgtggagggg accgctccag tcgtcctggc
     9661 cctggaggag atcctggagc tgcccctctc cggcgaggtc tccgtcaccg tccccgaggg
     9721 gtacgccttc tacgggctct accccgaaac ctatctcgaa gcggcccggc gggcggtgga
     9781 gctggaggag gggccggtcc aggtcatcgg catccgcagc atcggcacga gcctcgcggc
     9841 ggccgtggcg gccggagtgg gggacggcgc cacgaccgtc accgtccgcc cgggcgggca
     9901 tccgttctcg cggacgctgg cgatggggga ggagatggag cgccggctcc tgtccagacc
     9961 cggcgaggtc cgcttcgccg tggccgacga ggggccgggc ctctccggca gctctttcgg
    10021 gtcggtggcc gactggctgg aggagcgcgg cgtcctcccg gactccatct cgttcttccc
    10081 cagccatcgc ggcgggccgg ggccctacgc cagcgagcgc caccgggagc gctgggagcg
    10141 ggcgcgccgc cacaccgtgg acttcgagga gctgttcgtc gctcccggag ccagctggcc
    10201 cctggcgagc tgggtggcgg acctcaccgg ttccgccgag gggccgctcg aagacgtgag
    10261 cgccgggcgc tggcggcagc ggctcttccc aggcccgggc agccggcccc ctgcccatct
    10321 ccagcaggag cgccgcaaat acctgctgac cgccggcggc cggaaatggc tcctcaagtt
    10381 cgtggggctg gggcgctatg gccgcgaagc gctcgatctg gcccggacgc tggagagcaa
    10441 agggctcgtg ccgccggtgg ccgggttccg gcacggcttc ctggtggggc cctggctggc
    10501 cgatgaggac ggcgcccgcc ccctcccgct ggttccggac ctcgaccgcg ccgccctcct
    10561 cgagcaggtg gccacctacc tggcgttccg ggcggcgcgc ctccccgccg ggcccgagcg
    10621 gcggggcgcc gcgcccggga agctcctgga gatggcccgc ttcaacaccg ggaaggctct
    10681 ggggcccgag gccgcggagc ggctggacgc ctgggaggag cggctgccgg acctcgccgc
    10741 ccgggagcgc cccatcctct cggacaaccg cctgcacgcc tgggagtggc tggtcctccc
    10801 gggcgggcgg atcctcaagg ccgacgccct ggaccaccac cggagcaacg acctggtggg
    10861 ggagcaggac gtcgcctggg accaggccgg cgccgccatc gagctaaacc tgtccgagga
    10921 ggaaggggag cgcctgcgcg aggaggtggc gcggcggagc ggcgtcgtct ccaccacgga
    10981 ccaggtccgc ttctacaccc tgacctacct cgccttccag ctcggcgcct gccggctcgc
    11041 cgccgaggcc ctggagggga tggacggcg
//
LOCUS       DASPJI010000784         5884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_565073, whole genome shotgun sequence.
ACCESSION   DASPJI010000784 DASPJI010000000
VERSION     DASPJI010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565073"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VEL74_22050"
     CDS             <1..613
                     /locus_tag="VEL74_22050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65280.1"
                     /translation="DPPPATTESPESPDTAGQTLYARVGGQVFEARTRLAETVALPPA
                     EWRSPALTSFDIYQVGAVTVRDAQGEAVLQRAGTDWKRGNETISYVPVSDLLFTVTGA
                     RADRLLSVQEARGMGAALDRPALTLVLKPESPEGGGAAETLMLYPPVAQGVPARVSGR
                     DVVLLLPAGKLDEIRKNLDEVRKAKPVQAAKAEEGTEEGKAEP"
     gene            complement(687..2279)
                     /locus_tag="VEL74_22055"
     CDS             complement(687..2279)
                     /locus_tag="VEL74_22055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HYG65281.1"
                     /translation="MASRLFARKPLELLLEELKGENRLRRVLGPVQLTSLGVGAIIGA
                     GIFVATGAAAHNIAGPALMLSYVVAGITCIFAALCYAEFAAMVPVAGSAYTYAYATLG
                     ELFAWIIGWDLVLEYAVGSATVATGWSGYFQSVLAKIGIVLPATLQSSPWRYDVTTDS
                     FVGTGSFINLPAVIIVLLVTIVLVKGIQESASFNAAMVIIKVAAVLFVIAAGAFFINP
                     ENWKPFAPYGWTGLSFFGHPVAGQTAANGTPLGMLAGAAIIFFAYIGFDSVSTHTEEA
                     KNPQRDVPIGIVVSLVVCTLLYILVVAVLTGMVKYNELNVDAPVSDAFRQQGLGWAEG
                     LIAMAGVAGITSVLLVMMLSGPRVFMAMARDGLVPQKFFGDVHPKFRTPWKATILIGA
                     GVIAMAGFMPLDALLHLTNIGTLLAFVIVCAAVLIMRRKYPDAHRPFRCPWVPFVPIM
                     GILCCLMLMFSLPAENWLRLFAWLALGFVIYFPYGRRHSVMHNARSLQSEIAHHGATP
                     TATLPHHGVQTPPDDGPGAPKR"
     gene            complement(2334..3911)
                     /locus_tag="VEL74_22060"
     CDS             complement(2334..3911)
                     /locus_tag="VEL74_22060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HYG65282.1"
                     /translation="MSEAVASRPGQPPEAKTGFVRELGLLDSTMIVAGSMIGSGIFIV
                     PADIARQVGSPGWLLMVWVFSGLLTIAAALSYGELAAMMPKAGGQYVYLREAYSPLWG
                     FLYGWTLFLVIQTGTIAAVAVAFARFLGVLVPAISPTAWIIPPIDLSDSYAVSLSTQQ
                     GVAILLLAFLTWLNTRGIKLGKWIQNTFTSAKTLSLIVLILLGILVGRNAEAIAANFG
                     DLWTPRDAATLRPDFGWVPPVSAAAGAFGLFIAFCVAQVGSLFSSDAWNNVTFTAGEV
                     KDPKRTIPLSLAAGTILVISLYILANLAYLCTLPLAEIQNAPDDRVGTAALALIFGPA
                     GATIMAVAIMISTFGCNNGLILAGARVYYAMARDGLFFRRTGALNEHHVPAWGLILQC
                     AWASLLVLPRTRPRDASGAQLLDAAGNPQYGNLYSNLLDYVVFAVLIFYVLTIAGLFV
                     LRRKRPDAERPYRAVGYPVVPALYILAASVISVVLLVYKTETTWPGLAIVLTGIPVYF
                     LWHRRSRPAGATEETNP"
     gene            complement(4146..5795)
                     /locus_tag="VEL74_22065"
     CDS             complement(4146..5795)
                     /locus_tag="VEL74_22065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HYG65283.1"
                     /translation="MAKGESWGTRVGLVLAMAGNAVGLGNFLRFPAQAAQNGGGAFLI
                     PYLISFVLMGIPLLWVEWAIGRHGGRFGHHSAPGMFDRLGRGKWLKYVGVFGLFTNIT
                     VAAYYCYIESWTLAYVWHSLIGTFKEVAPTEFFPRYLGQHEDSFFALPPEAFFWFVLT
                     LALNTFILSRGLAKGIELAAKIGMPLLLIFAAILAIRGLGLDPGSDPGVTQSPLEGLN
                     FVWNPNLSGLSNPNTWLAAAGQVFFTLSMGMGSIHCYASYVKHRQDIALNAASAGWMN
                     EFVEVVLGGSLLIPIATAYLGLAAVQEATAGGSGFGLGFLTLPTLFNNWGAFAPIAGA
                     MWFGLLFFAGITSSLAMGQPIMAFLEDEFGTKRNRAAVLFGLGVFTLGFWCVWLYPGG
                     AFDEFDFWTGTFSLVVFALAEAFIFAYVFGIDRGWEEITRGADIKVPPIFRYVIKYVT
                     PIFILVVFVGALLNPAGGDWGAAFGSLFSGDGWPFDPGSVIGRILHTGTGYAWFDDTG
                     RGTRVLVQDLTRILLMLVFAFWSFMVWKAWKIKRDKQRSPA"
BASE COUNT         1071 a         1865 c         2160 g          788 t
ORIGIN      
        1 cgatccgcca ccggccacca ccgagtcccc cgaatctccg gacaccgccg ggcagaccct
       61 ctatgcccgc gtcggcggcc aggtcttcga ggcccgcacc cgcctggccg agaccgtggc
      121 gttgccgccg gccgagtggc gctcgcccgc gctcacctcg ttcgacatct atcaggtcgg
      181 ggcggtcacc gtgcgcgacg cccaggggga ggccgtcctg cagcgcgccg gtaccgactg
      241 gaagcggggg aacgagacga tctcctacgt gccggtgagc gacctgctct tcaccgtgac
      301 cggcgcccgg gccgaccgtc tcctctccgt ccaggaggcg cgggggatgg gcgcggcgct
      361 ggaccggccg gcgctcaccc tggtcttgaa gcccgaatct cccgagggag ggggcgccgc
      421 cgagacgctg atgctctatc cgccggtcgc ccagggggtg ccggcccggg tgagcggccg
      481 cgacgtggtg ctgctcctcc ccgccggcaa gctcgacgag atccgcaaga acctggacga
      541 ggtccgcaag gcgaagccgg tgcaggcggc gaaggcggag gaggggacgg aggaggggaa
      601 ggcggagccg taggcttctc cgggtggaaa agaaaaaggc cggcgcctcc ggggaggcgg
      661 ccggcctttt tgcttcggcc tcgtgctcag cgcttcggcg cgcccggtcc gtcgtcaggg
      721 ggagtctgca ccccgtggtg gggcagggtg gcggtggggg tggcgccgtg atgggcgatc
      781 tcgctctgca gcgagcgggc gttgtgcatc acgctgtggc gccggccgta ggggaagtag
      841 atgacgaagc cgagggccag ccaggcgaag aggcgcagcc agttctcggc gggcagcgag
      901 aacatgagca tcaggcagca gaggatgccc atgatcggaa cgaagggtac ccaggggcag
      961 cggaacggcc ggtgggcgtc cgggtacttg cggcgcatga tgagcaccgc cgcgcagacg
     1021 atgacgaaag ccagcagcgt gccgatgttc gtcaggtgga gcagcgcgtc gagcggcatg
     1081 aagccggcca tggcgatgac gccggcgccg atcaggatcg tcgccttcca gggggtgcgg
     1141 aacttcgggt ggacgtcgcc gaagaacttc tgcggcacca agccgtcgcg cgccatggcc
     1201 atgaacaccc gggggccgga gagcatcatg accaggagaa ccgaggtgat gccggcgacg
     1261 ccggccatgg cgatgagccc ttccgcccag ccgagcccct gctgcctgaa ggcgtcgctc
     1321 accggggcgt cgacgttcag ctcgttgtac ttcaccatgc cggtcagcac ggcgacgacc
     1381 aggatgtaga ggagggtgca gaccaccagc gacacgacga tgccgatcgg cacgtcgcgc
     1441 tgcgggttct tcgcctcctc ggtgtgcgtc gacaccgagt cgaagccgat ataggcaaag
     1501 aagatgatgg ccgcgccggc gagcatcccc agcggggtgc cgttggctgc cgtctggccg
     1561 gccacgggat ggccgaagaa gctcaggccg gtccagccgt agggcgcgaa cggcttccag
     1621 ttctcggggt tgatgaagaa ggcgccggcg gcgatgacga agagcaccgc ggccaccttg
     1681 atgatcacca tggcggcgtt gaagctggcg ctctcctgga tgcccttgac caggacgatg
     1741 gtgaccagga ggacgatgat caccgccggc aggttgatga acgagccggt gccgacgaac
     1801 gagtcggtgg tcacgtcgta gcgccagggc gaggattgga gcgtcgctgg cagcacgatc
     1861 cctatcttcg cgaggacgct ctggaagtag cctgaccacc ctgtcgcgac ggtggcgctg
     1921 ccgaccgcgt actccagcac caggtcccaa ccgatgatcc aggcgaacag ctcgcccagg
     1981 gtggcgtagg cataggtgta ggccgacccg gccaccggca ccatggcggc gaactccgcg
     2041 tagcagagcg ccgcgaagat gcaggtgatg ccggcgacga cgtaggagag catcagggcc
     2101 gggccggcga tgttgtgggc cgccgcgccg gtcgcgacga agatgccggc gccgatgatt
     2161 gcgccgacac ccaggctggt gagctgcacg ggtccgagga cccggcgcag ccggttctcc
     2221 cccttgagct cctcgaggag gagctcgagc ggttttctgg caaaaaggcg actcgccatg
     2281 gggtgtctag acctccagaa caggaaagag ggtcaggaac aggactcctt gaatcaaggg
     2341 ttcgtctcct ccgtcgcgcc ggccggccgg gaccgccggt gccagaggaa gtagacgggg
     2401 atgccggtca gcacgatcgc caggccgggc caggtggtct ccgtcttgta gaccagcaga
     2461 accaccgaga tgacggacgc ggccaggatg tacagcgccg gcaccaccgg atagccgacg
     2521 gcgcggtacg ggcgctcggc gtccggccgc ttgcggcgca gcacgaacag cccggcgatg
     2581 gtcagcacgt agaagatcag cacggcgaag accacgtagt ccagcaggtt gctgtacagg
     2641 ttgccgtact gcgggttgcc cgccgcgtcc aggagctgcg ccccggaggc atcgcgcggg
     2701 cgggtgcggg ggagcaccag cagcgacgcc caggcgcact gcaggatcag gccccacgcc
     2761 ggcacgtgat gctcgttgag cgccccggtg cgccggaaga acaggccgtc ccgggccatg
     2821 gcgtagtaga cccgggcacc ggccaggatc aggccgttgt tgcagccgaa ggtggagatc
     2881 atgatcgcca ccgccatgat cgtcgccccg gccgggccga agatcagcgc cagggcggcg
     2941 gtgcccaccc ggtcgtccgg cgcgttctgg atctccgcca gggggagggt acacaggtag
     3001 gccaggttcg ccaggatata gagcgagatc accaggatgg tgccggcggc gagcgacagc
     3061 gggatcgtgc gcttcgggtc cttgacctcg ccggcggtga aggtgacgtt gttccaggcg
     3121 tccgacgaga agagggagcc cacctgcgcg acgcagaagg cgatgaacag gccgaacgcc
     3181 ccggccgctg cggacaccgg cggcacccac ccgaagtccg gccggagggt ggccgcgtcg
     3241 cgcggcgtcc agaggtcgcc gaagttggcg gcgatggcct cggcgttgcg gcccaccagg
     3301 attcccagca ggatcagcac gatgagcgac agggtcttgg ccgaggtgaa ggtgttctgg
     3361 atccacttgc ccagcttgat cccgcgggtg ttcagccagg tgaggaacgc caggagcagg
     3421 atggcgaccc cctgctgcgt cgacagcgag acggcatagc tgtccgacag gtcgatgggc
     3481 gggatgatcc acgctgtcgg cgagatggcg gggacgagga cgccgaggaa gcgggcgaag
     3541 gccacggcca ccgcggcgat ggtgccggtc tggatcacca ggaacagcgt ccagccgtag
     3601 aggaaccccc agagcggcga gtaggcctcc cgcaggtaga catactggcc gcccgccttg
     3661 ggcatcatcg ccgccagctc cccataggag agcgcggcag caatggtgag caggccggag
     3721 aacacccaga ccatgagcag ccagcccggc gaccccacct gccgggcgat gtcggccggc
     3781 acgatgaaga taccggagcc gatcatcgac ccggccacga tcatggtgga gtcgagcagg
     3841 cccagctccc ggacgaaccc cgtcttcgcc tcaggcggct gaccggggcg cgaggcaacg
     3901 gcttcggaca tggtgcctcc agaaagatgc acaatcctac aggtgccgct aggccttgtc
     3961 acgtggacgt tacagggagg gctgaaagag aagaccccgg cggggcgccg gggtcttcgg
     4021 ggggaacggg gcggcctatt cggcgtcgtc ccggggcggc gtggtcagca ccttccagaa
     4081 gaatttgaag gtgaagaaga agatcacggc ccaggtgatc accagcatgg tccaggcggc
     4141 gctcgtcatg cggggctcct ttgcttgtcc ctcttgatct tccaggcctt ccagaccatg
     4201 aaggaccaga aggcgaagac cagcatcagc aggatacggg tgaggtcctg caccagcacg
     4261 cgggtgccgc ggccggtgtc gtcgaaccag gcgtagccgg tgccggtgtg gaggatccgg
     4321 ccgatgacgc tgccggggtc gaacggccag ccgtcgcccg agaacagcga gccgaaggcc
     4381 gccccccagt ccccgcccgc cgggttgagc agcgcgccca cgaacaccac caggatgaag
     4441 atgggcgtca cgtacttgat gacgtacctg aagatgggag gcaccttgat gtcggcgccg
     4501 cgggtgatct cctcccagcc gcggtcgatg ccgaagacgt aggcgaagat gaaggcctcg
     4561 gccagggcga agacgaccag cgagaaggtg ccggtccaga agtcgaattc gtcgaaggcg
     4621 ccgcccggat agagccagac gcaccagaag ccgagggtga agacgcccag gccgaagagc
     4681 accgcggccc ggttgcgctt cgtcccgaac tcgtcctcca ggaaggccat gatcggctgc
     4741 cccatggcga gcgacgaggt gatgccggcg aagaacagca ggccgaacca catggcgccg
     4801 gcgatggggg cgaaggcgcc ccagttgttg aacagcgtcg gcagggtgag gaagccgagg
     4861 ccgaagccgc tcccccccgc cgtcgcctcc tgcaccgcgg cgaggcccag ataggcggtg
     4921 gcgatgggga tgagcagcga gccgcccagc accacctcga cgaactcgtt catccagccc
     4981 gccgacgcgg cgttcagggc gatgtcctgc cggtgcttga cgtaggaggc gtagcagtgg
     5041 atggagccca tgcccatcga cagggtgaag aagacctgcc ccgccgccgc cagccaggtg
     5101 ttcggattgc tcaggcccga gaggttgggg ttccagacga agttgagccc ttcgagcggg
     5161 ctctgggtga cgcccgggtc gctgccggga tcgaggccca ggccgcggat cgccaggatg
     5221 gcggcgaaga tcagcagcag cggcatgccg atcttggccg cgagctcgat ccccttggcc
     5281 aggccgcggg agaggatgaa ggtgttgagc gccagcgtga ggacgaacca gaagaacgcc
     5341 tccggcggca gggcgaagaa ggagtcctcg tgctgcccca ggtagcgggg gaagaactcg
     5401 gtgggggcga cctccttgaa ggtgccgatc agcgagtgcc agacgtaggc cagggtccag
     5461 gactcgatgt agcagtagta ggcggccacc gtgatgttgg tgaacaggcc gaagacgccg
     5521 acgtacttga gccacttgcc gcggcccagc cggtcgaaca tcccgggggc cgagtggtgg
     5581 ccgaaccgcc cgccgtgccg gccgatggcc cactccaccc agaggagcgg gatgcccatg
     5641 aggacgaacg agatcagata ggggatcagg aacgctccgc cgccgttctg ggccgcctgc
     5701 gccgggaaac gcaggaagtt gcccaggccg acggcgttgc cagccatggc caggaccagg
     5761 ccgacgcgcg taccccagct ttcgcctttg gccactatgc gccctcctcc gtagttgggc
     5821 gcctatccta gcctgggagc ggggtggctg tcaccgttgc ggccggggga gcggggcgcc
     5881 gaac
//
LOCUS       DASPJI010000785         7245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_450943, whole genome shotgun sequence.
ACCESSION   DASPJI010000785 DASPJI010000000
VERSION     DASPJI010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7245)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7245
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450943"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VEL74_22070"
     CDS             complement(<1..476)
                     /locus_tag="VEL74_22070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HYG65284.1"
                     /translation="MPIDLYQVDAFTDRAFAGNPAAVCLLPEPREADWMQRVAREMNL
                     SETAFLVARGGGFDLRWFTPATEVELCGHATLASAHVLWQAGRLGLAELAHFHTLSGL
                     LTARRRGDGWIELDFPARAAEPLDASSAPPGLVEALGADVPVFLGQSRYDYLLELPS"
     gene            689..1852
                     /locus_tag="VEL74_22075"
     CDS             689..1852
                     /locus_tag="VEL74_22075"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HYG65285.1"
                     /translation="MGLSSAASSTAGSWREAEGFPGLLEGFRQGLERDLEAWLAAKRD
                     EAADQGSDGMLELIDGVAQLVTRGGKRLRPALVYYTYRACGGRSDGEVLPLALATELL
                     HTYLLIHDDIMDHADVRRGQPAAHVRFREAHRQRRLRGDSEDFGRSVAILLGDLAHTW
                     AVELATLVPLDGGRSPQIAREISRCFSAMSQEVIGGQYLEILLAQRRPDPGAEPAPEQ
                     AEELMRILRLKSGRYTAERPIQLGGLLAGAAPDVLAALSDYGTAMGEAFQLQDDLLGM
                     FGDAGTVGKPVGADLKEGKFTFLIYHALAAASPAERRLLEASLGDPGLDARAVDDARR
                     VIEETGARETVDAMVSKRLAAARAALGRIAPGLEPRGRLFLEGLIDYLRERKQ"
     gene            1849..2874
                     /gene="fni"
                     /locus_tag="VEL74_22080"
     CDS             1849..2874
                     /gene="fni"
                     /locus_tag="VEL74_22080"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015150569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HYG65286.1"
                     /translation="MSVEVIVDEERDRKAEHITLALDRRMQLDANYFDEYRFGHEALP
                     EIDIADVDTSVEFLGRRLAAPLLISSMTGGTAAAGVINRNLAAGAERTGVALGIGSQR
                     KALEDPAKADTFRVRDAAPSIPLLANLGAVQLNYGMGIAECQAAVDMIGADALILHLN
                     PLQEAIQPEGQCDFAGLLPRIGEVVRALPVPVVAKEIGCGLSTRAARALAGQGVRIID
                     TAGVGGTSWARIEAARAGDLDLGETFAGWGIPTPVSIQQVRQVPGITVIASGGVRNGI
                     DAAKALALGADLVGLAYPFLQAATESAERVAERIRRIVRELQICMFCLGVKTIAELKT
                     VELHKGS"
     gene            2876..4159
                     /gene="hmgA"
                     /locus_tag="VEL74_22085"
     CDS             2876..4159
                     /gene="hmgA"
                     /locus_tag="VEL74_22085"
                     /EC_number="1.1.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase (NADPH)"
                     /protein_id="HYG65287.1"
                     /translation="MSKSQSAAASAPLTREDLVRSLVGGERRFHELPKDLSPEEAAEI
                     RRQALEQLTQTPLDNIGHHSLDVVRASHRNCENFIGVAQIPMGVVGPLKVRGTAADGE
                     FYVPMATTEAALIASTNRGCAAIREAGGAVVRVEDVGMTRAPVFRTSGIVQTQQFLQW
                     IKDHEDDLRRLTESTSKYLKLLEVRPFAFGTTVFLRFRFDSGDAMGMNMVTIACDRAV
                     NELIEPATGVACVALSGNYCVDKKPAAINWQEGRGKRIYAEVLVEAPTLHRYLKTTAR
                     DLAEVQYRKNLLGSIAAGSMGFNAHYANVLAAFFIATGQDPAHVVEGSLGITCIEPRG
                     PDSVFASIFMPDVPLGAVGGGTALDTQREALAVLGVSPDPERRGEAVTRLAEILGAVV
                     LAGELSLMAAFTSQDLARAHEKLGRGELPPRPIPS"
     gene            4217..5302
                     /locus_tag="VEL74_22090"
     CDS             4217..5302
                     /locus_tag="VEL74_22090"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65288.1"
                     /translation="MLPVSISTPGKLILMGEHAAVYGRPALVAAIDRRLTARFSAAPR
                     DVRLELPGLGHAEETSWPAILGYARDARRRWDEYAREPGSEAFGRVRGEDPAHVVKVA
                     LGEAAAALGGKGGRPALTLRVDSELPIGSGFGSSAALAVAVIGGALLALGPAADAADP
                     ATVERLALEVERRQHGQPSGVDGATVVHGGVVWARRLGADRLEREPVAVRSPLLSRLH
                     VFDTGTPPESTGAVVAAVRDRRDRDPGRHERLLDRMEAATRAFREELARELDESDQIV
                     RLLQEQEACLEELGVVPLPIREVVRRVEAEGGAAKISGAGSLSGPGAGSLLVYHSDRE
                     RVAGWKFLRPFRFHSVQLGAPGFRREG"
     gene            5305..6327
                     /gene="mvaD"
                     /locus_tag="VEL74_22095"
     CDS             5305..6327
                     /gene="mvaD"
                     /locus_tag="VEL74_22095"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HYG65289.1"
                     /translation="MSKTTVVAPANIAFVKYWGARDTERAIPFNRSISMTLERCHSVC
                     TAEVISEERDQVWLAEPDGGFRAPEPEFDRRVREHLDRLRQWSGRNDRLWVATRNSFP
                     SAAGLASSASGFAALTMAAAGALGKGNAPIEQLSRLARLSGSGSAARSILGGFVEWPA
                     HNVDLNAPDADEKAFAYQIADADHWDLRDVIAVVELGPKVVSSLEGHRRAPTSPYFEK
                     RQEILPERTRLVREAIEEQDLDTLGPLIEEESIDLHMIAMTSKPSIFYWSPGTVAVLK
                     AVSELRQEGLSAWATLDAGANVHVICDPDSEQDVVDRLENLPAVGFLIRDGVGPGPDH
                     DEEHLF"
     gene            6340..7134
                     /locus_tag="VEL74_22100"
     CDS             6340..7134
                     /locus_tag="VEL74_22100"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HYG65290.1"
                     /translation="MTAGILIKLGGSLLTEKSRPETPRTEVIARLAAEIARAAARGPE
                     RLILGHGSGSFGHVAASRSRLAGGLTSADQLPGISATQQSAAALHRLVVEALAAAGAT
                     PYSIAPSSCVVTAGGRPAAFADEPLLLALERGLLPVLYGDVVLDRQQGVAICSTETIL
                     ELLARTLPARGVPVSRAIWLGETDGLYDEAGRTIPRVSAATFQEAARGIGAPAGTDVT
                     GGMRHRVEAALALAGLGIPSLLANGLTPGLLERALRGEAVPGTEVV"
BASE COUNT         1002 a         2677 c         2566 g         1000 t
ORIGIN      
        1 gaggggagct ccagcaggta gtcgtagcgg ctttggccga ggaagaccgg cacgtccgcc
       61 cccagcgcct ccaccaggcc cggcggagcg ctgctcgcgt cgagcggctc ggcggcacgg
      121 gccgggaagt cgagctcgat ccagccgtcg ccccggcgcc gggcggtgag cagcccggag
      181 agggtgtgga agtgggccag ctccgccagc cccagccgcc ctgcctgcca gagcacgtgg
      241 gcgctcgcca gggtggcgtg gccgcacagc tccacctccg tcgcgggggt gaaccagcgc
      301 aggtcgaagc cgccgccccg ggcgaccagg aacgccgtct cggagaggtt catctcgcgg
      361 gccacccgct gcatccagtc cgcctcccgc ggctcgggca gcaggcagac cgccgccggg
      421 ttgcccgcga aggcgcggtc ggtgaaggca tctacctgat agaggtcgat cggcatggag
      481 ctagggtatc gcaacgcgtc ttcgcgccgc accgctttgc atcgcaaagt gctctcaggt
      541 acaatgcctg caccgggagc tcaggaggag gcccggcggg aacggagccg ggaaatttcg
      601 ggttacactc tgcggcggtg aggtgtgggt gcgcggtgcc ctgtgttttc ccgaagcaga
      661 tcgagtgcga ggagacgagt taaacggcat gggactatcc agcgcggcat cttcgacagc
      721 cggctcctgg cgggaggccg agggcttccc cgggctgctc gagggtttcc ggcaggggct
      781 cgagcgcgac ctcgaggctt ggctcgccgc caagcgtgac gaggccgccg accagggctc
      841 ggacgggatg ctcgagttga tcgacggcgt cgcccaactg gtcacccgcg gcggcaagcg
      901 gctgcgtccg gcgctggtct actacaccta ccgcgcctgc ggcggccgct ccgacgggga
      961 ggtcctcccc ctcgccctcg ccaccgagct cctccacacc tatctattga tccacgacga
     1021 catcatggat cacgccgacg tccggcgcgg ccagccggcg gcgcacgtgc gcttccgcga
     1081 ggcgcaccgg cagcggcgcc tgcgcggcga cagcgaggac ttcggccgct ccgtggcgat
     1141 cctgctcggc gacctcgccc acacctgggc ggtggagctg gccaccctgg tccccctgga
     1201 cggcggccgc tctccccaga tcgcgcgcga gatctcgcgc tgcttctcgg ccatgagcca
     1261 ggaggtgatc ggcggccagt acctggagat cctcctcgcc cagcgccgcc ccgaccccgg
     1321 cgcggagccc gctcccgagc aggccgagga gctgatgcgg atactgcgct tgaagtccgg
     1381 gcgctacacc gccgagcggc cgatccagct cggcggcctg ctggcggggg cggcgccgga
     1441 cgtcctggcg gcgctgtccg actacggcac ggccatgggc gaggccttcc agctccagga
     1501 cgacctgctg ggaatgttcg gcgacgcggg caccgtcggc aagcccgtgg gggccgacct
     1561 caaggagggc aagttcacct tcctcatcta tcacgccctg gccgcggcct cgccggcgga
     1621 gcggcggctc ctggaggcgt cgctcgggga tcccgggctc gacgcccgcg ccgtcgacga
     1681 cgcccgccgg gtcattgagg agaccggcgc gcgcgagacg gtcgacgcca tggtgagcaa
     1741 gcgcctcgcc gccgcccgcg cggccctcgg caggatcgcg cccggcctcg agccgcgggg
     1801 acgcctcttc ctggaggggc tgatcgacta cctgcgggag cgcaagcagt gagcgtagag
     1861 gtgatagtgg acgaggagcg cgaccgcaag gcggagcaca tcacgctggc gctcgaccgc
     1921 cgcatgcagc tcgacgccaa ctacttcgac gagtaccggt tcggtcacga ggcgctgccc
     1981 gagatcgaca tcgccgacgt cgacacctcg gtcgagttcc tcggccgccg cctggcggcg
     2041 ccgctcctca tctcgtcgat gacgggaggc acggccgccg ccggggtgat caaccgcaac
     2101 ctcgccgccg gcgccgagcg caccggcgtg gccctgggca tcggctcgca acggaaagct
     2161 ttggaagacc cggcgaaagc tgataccttt cgcgtccgcg atgccgctcc atcgatcccc
     2221 ctgctggcga acctcggtgc cgtgcagctc aactacggca tggggatcgc ggagtgccag
     2281 gcggcggtgg acatgatcgg ggccgacgcc ctgatcctcc acctgaaccc gctgcaggag
     2341 gcgatccagc ccgaggggca gtgcgacttc gccggcctcc tgccgcggat cggcgaggtg
     2401 gtgcgcgccc tgccggtgcc ggtcgtggcg aaggagatcg gctgcggcct ctccacccgc
     2461 gccgcgcggg cgctggccgg ccagggggtg cgcatcatcg acaccgccgg ggtcggcggc
     2521 acgagctggg cgcgcatcga ggccgcccgc gccggcgacc tcgatctggg tgagacgttc
     2581 gccggctggg ggatccccac gccggtttcg attcagcagg tccggcaggt gccggggatc
     2641 accgtgatcg ccagcggcgg ggtgcgcaac ggcatcgacg ccgccaaggc cctggcgctg
     2701 ggcgccgacc tggtgggatt ggcttacccc ttcctccagg ccgccaccga atcggcggag
     2761 cgggtggccg agcggatccg ccgcatcgtc cgggagctgc agatctgcat gttctgcctg
     2821 ggagtgaaaa ccattgcgga gctcaagacc gtcgagctcc ataaagggtc gtgagatgag
     2881 caaaagccag tccgccgccg cctccgcccc cctgacccgg gaggacctcg tccgctcgct
     2941 ggtgggggga gagcgccgct ttcacgagct gcccaaggac ctctcccccg aggaggcggc
     3001 ggagatccgc cgccaagccc tggagcagct cacgcagacg cccctcgaca acatcggcca
     3061 tcactcgctc gacgtggtcc gcgcctcgca ccgcaattgc gagaacttca tcggcgtcgc
     3121 ccagatcccc atgggggtgg tcggcccgct gaaggtgcgc ggcaccgccg ccgacggcga
     3181 gttctacgtc cccatggcca ccaccgaggc ggcgctgatc gccagcacca accggggctg
     3241 cgccgccatc cgcgaggccg gcggcgccgt cgtgcgcgtc gaggacgtgg gcatgacccg
     3301 cgcccccgtc ttccgcacct cgggcatcgt ccagacgcag cagttcctgc agtggatcaa
     3361 ggaccacgag gacgacctgc ggcgcctgac cgagtccacc agcaagtatc tgaagctgct
     3421 ggaggtccgc cccttcgcct tcggcaccac ggtgttcctg cgcttccgct tcgactcggg
     3481 cgacgccatg ggcatgaaca tggtgaccat cgcctgcgac cgggcggtca acgagctcat
     3541 cgagccggcg accggcgtcg cttgcgtcgc cctgtcgggc aactactgcg tcgacaagaa
     3601 gccggcggcg atcaactggc aggaggggcg gggcaagcgg atctatgccg aggtgctggt
     3661 cgaggcgccg acgctccacc gctacctcaa gaccacggcc cgcgacctcg ccgaggtgca
     3721 gtaccggaag aacctcctgg gttccatcgc ggccggctcc atgggcttca atgcccacta
     3781 cgccaacgtg ctcgccgcct tcttcatcgc caccgggcag gacccggcgc acgtggtcga
     3841 gggctcgctc ggcatcacct gcatcgagcc gcgggggccg gactcggtct tcgcctccat
     3901 cttcatgccc gacgtgccgc tcggcgcggt gggcggcggc accgcgctgg acacccagcg
     3961 cgaagccctg gccgtgctcg gcgtctcccc cgatccggag cgccgcggcg aggccgtgac
     4021 gcgcctcgcc gagatcctcg gcgccgtggt cctcgccggc gagctgtcgc tgatggccgc
     4081 cttcacctcc caggacctgg cgcgtgccca cgagaagctg gggcgcggcg agctgccccc
     4141 ccgtccgatt ccgagctgag gcccacgcgg ccggccctgc cgccctcatg gggcgcgggc
     4201 cggcctccct ctccccgtgc tccccgtctc catctcgacc cccggcaagc tgatcctcat
     4261 gggcgagcac gccgccgtct acggccggcc cgccctggtc gcggcgatcg accgccggct
     4321 gacggcccgc ttctcggcag ccccgcggga cgtccgcctc gagttaccag gtctcgggca
     4381 tgccgaggag acctcctggc ccgccatcct gggatatgct cgggatgccc ggcggcgctg
     4441 ggacgagtac gcccgcgagc ccggatccga agccttcggg cgggtgcgcg gcgaggaccc
     4501 ggcgcacgtg gtcaaggtcg ccctcgggga ggccgcggcg gccctggggg ggaaaggcgg
     4561 ccggcccgct ctcaccctcc gcgtcgactc ggagctgccc atcggctccg gcttcggcag
     4621 ctccgcggcg ttggccgtcg cggtgatcgg cggcgcgctg ctcgcgctcg ggcctgctgc
     4681 cgacgcggcc gatccggcga ccgtcgagcg cctggcgctg gaggtggagc gccgccagca
     4741 cggccagccc tccggggtgg acggcgccac ggtcgtccac ggcggcgtgg tgtgggcgcg
     4801 gcggctgggg gcagaccggt tggagcggga gccggtggcg gtgcgctctc ccctgctctc
     4861 gcgcctgcac gtcttcgaca ccggcacgcc gccggagtcc acgggcgcgg tggtggcggc
     4921 ggtgcgcgac cgccgggacc gcgatcccgg ccggcacgag cgcctgctcg accgcatgga
     4981 ggcggcgacc cgggccttcc gcgaggaact ggcacgagag ttggatgagt ccgaccagat
     5041 agtccgtctg ctgcaggagc aggaggcgtg cctcgaggag ttgggggtcg tgccactgcc
     5101 gattcgtgaa gtcgtgagac gcgtggaagc cgaaggcgga gccgccaaga tctccggcgc
     5161 cggctcactc tcgggaccgg gcgcggggtc gctcctggtc tatcattccg atcgcgaacg
     5221 cgtcgcgggc tggaagttcc tgcggccgtt ccgtttccat tcagtccaac tcggagcccc
     5281 ggggttccga agggagggtt gacgatgagc aagaccaccg tcgtcgcgcc ggccaacatc
     5341 gccttcgtca agtactgggg cgccagggat accgagcggg ccattccgtt caaccgttcg
     5401 atctccatga ccctcgaacg ttgccactcg gtgtgcaccg ccgaggtgat ctccgaggag
     5461 cgggaccagg tctggctggc ggagccggac ggcggcttcc gcgcgccgga gcccgagttc
     5521 gaccgccgcg tccgcgagca cctggaccgc ctccggcagt ggtccggccg caacgaccgg
     5581 ctctgggtgg cgacccgcaa cagcttcccc agcgccgccg gcctcgcctc ctccgcgtcc
     5641 ggcttcgcgg ccctgaccat ggccgcggcc ggcgccctgg gcaaggggaa cgcccccatc
     5701 gagcagctct cccggctcgc ccgcctgagc ggctccggct cggcggcccg ctcaatcctc
     5761 ggcggcttcg tcgagtggcc ggcccacaac gtcgacctca acgccccgga tgccgacgag
     5821 aaggccttcg cctaccagat cgccgacgcc gaccattggg acctgcgcga cgtcatcgcc
     5881 gtggtggagc tgggccccaa ggtggtctcc tcgctcgaag ggcaccgccg cgcccccacc
     5941 agcccctact tcgagaagcg ccaggagatc ctccccgagc gcacccgcct ggtgcgcgag
     6001 gcgatcgagg agcaggacct cgacaccctc gggccgctca tcgaggagga gtcgatcgac
     6061 ctccacatga tcgcgatgac ctcgaagccg tccatcttct actggagccc gggcacggtg
     6121 gcggtgctca aggcggtctc cgagctgcgc caggaggggc tctccgcctg ggcgacgctg
     6181 gacgccggag ccaacgtcca cgtgatctgc gaccccgact cggagcagga cgtagtggac
     6241 cgcctggaga acctgccggc ggtaggtttc ctcatccgcg acggcgtcgg ccccggcccg
     6301 gaccacgacg aagagcacct cttctgagat gaccgcccga tgaccgccgg catcctgatc
     6361 aagctcggcg gcagcctgct gacggagaaa tcccggcccg agacgccgcg caccgaggtc
     6421 atcgcccggc tcgcggcgga gatcgcccgg gccgcggcgc gcggccccga gcggctgatc
     6481 ctcggccacg gcagcggctc cttcggccac gtcgccgcca gccgctcgcg cctcgccggc
     6541 ggcctgacgt cggcggacca gctccccggc atctccgcca cccagcagag cgccgccgcc
     6601 ctccatcgcc tggtggtgga ggcgctcgcc gcagcgggcg cgacccccta ctccatcgcc
     6661 ccgtcgagct gtgtggtgac ggcaggcggc cggcccgccg ccttcgccga cgagccgctc
     6721 ctgctggcgc tcgaacgggg gctgctgccg gtgctctacg gcgacgtggt gctggaccgg
     6781 cagcaggggg tcgccatctg ctcgacggag acgatcctcg agctcctcgc ccggacgctg
     6841 ccggcgcgcg gcgtgccggt gagccgggcg atctggctgg gcgagaccga cggcctgtac
     6901 gacgaggccg gccgcaccat cccccgggtc tccgcggcca ccttccagga ggcggcgcgg
     6961 ggcatcggcg ccccggcggg caccgacgtg accggcggca tgcgccaccg cgtcgaggcc
     7021 gccctcgcgc tcgccggcct gggcatcccg tcgctcctcg ccaacggcct cacccccggc
     7081 ctgctggagc gggctctgcg gggagaggcg gtgccgggga ccgaagtggt ctgatatcct
     7141 ctgccgcgtg tcgacagcgg acgaggcgga cgagatgagc ggggaggact tggagcggct
     7201 cctgatcgcc accagccgca cgttcgcgct cgccatcccg ctgct
//
LOCUS       DASPJI010000786         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_728629, whole genome shotgun sequence.
ACCESSION   DASPJI010000786 DASPJI010000000
VERSION     DASPJI010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728629"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2257)
                     /locus_tag="VEL74_22105"
     CDS             complement(<1..>2257)
                     /locus_tag="VEL74_22105"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG65291.1"
                     /translation="RGLRIELGEIEAVLSAHPQVREAAVLVREERRGEPYLVAFLASR
                     SEVGPDELRGWLRGRLPESMVPPVFVRLDSLPLTPNGKLDRKALAAIAPARHERDRLE
                     TYVAPSSLTEEVLAGIFAEVLRHERVGVRNGFFDLGGHSLLATQVTSRVREVFGVELP
                     LRELFEEPTVAGLAGRLERLRESTVPAPPIVPIPRSLGEDGGLPLSFAQQRLWFLERL
                     APGSPAYNVPLAVRISGLLSPALLGEALEEVARRHEALRTAFAEREGRPIQVIAPSVR
                     LPLAMLDLSGLPESRRETELVCRATAEALRPFDLGTRPLVRAALLRLASDDHALLVTM
                     HHIVTDGWSMGIFLRELTALYGGLEVPEPPVQYADFAVWQRAWLQGNVLEAQLGYWRQ
                     RLAGAPARLILPTDRPRPVVETHRGDSRPLDLTPALARSVSALARREGATPFMVLLAA
                     FAALLGRYSGQDDVVVGSPIAGRNRRETESLIGLFVNTLALRVELTGAPDFLGLLRQA
                     RRTALDAYAHQDVPFERLVEELVTERELGHSPLFQVMLMVQNTPALAAGGAPMRELEL
                     RPLEVESEIAKFELTLTLTPLGEELTGALEHNTDLFDGATAERLARHFAALLEGAVAA
                     PESPLPEIPLLAAAERHQLLGEWNDTEIGFPEGGRCVHELIQEQARRTPEALALSFEG
                     EDLTYADLEARANRLAHHLRRLGAGPGTLVGVSLERSAAMVAALLGVLKSGSAYVPLD
                     PTYPKDRIAYVV"
BASE COUNT          354 a          853 c          756 g          294 t
ORIGIN      
        1 caaccacgta ggcgatccgg tccttgggat aggtgggatc cagtggcacg tatgccgagc
       61 cggacttgag gactcccaga agtgccgcca ccatggcggc cgagcgctcc agcgacacgc
      121 ctaccagagt cccgggcccc gctcccaacc gccgcaggtg gtgggccaag cgattcgccc
      181 gagcttcgag atcggcatag gtcaggtctt cgccctcgaa gctcagggcg agggcctccg
      241 gggtgcggcg cgcctgctcc tggatcagct cgtgcacgca ccggccaccc tccgggaagc
      301 cgatttccgt gtcgttccac tcccccagga gctggtgccg ttcggccgct gccagcaggg
      361 ggatctccgg cagcgggctc tccggcgccg ccaccgctcc ctccagcagt gccgcgaagt
      421 gcctcgccag ccgctccgcc gtggcaccgt cgaaaagatc cgtgttgtgc tccagggcac
      481 cggtcagctc ctcgcccaga ggagtgaggg tcagggtcag ctcgaacttc gcgatctccg
      541 actccacctc caacggtctg agctcgagct cccgcatcgg ggctccccct gccgcgagcg
      601 ccggcgtatt ctgcaccatc aacatcacct ggaacagggg ggagtgcccg agctcgcgtt
      661 cggtcaccag ctcctcgacc agccgctcga acggaacgtc ctggtgggcg taggcgtcca
      721 gcgccgtacg ccgcgcctgg cgcagcaggc ccaggaagtc cggggcgccc gtcagctcca
      781 cccgcagcgc cagcgtgttc acgaacagtc cgatgagact ctcggtctcc cgccggttcc
      841 gcccggcgat gggcgagccc acgaccacgt cgtcctggcc ggagtagcgg ccgagcagag
      901 cggcgaacgc cgccaggagg accatgaacg gtgtcgcccc ctcgcgccgt gccagcgccg
      961 acaccgagcg cgccagtgcc ggggtcaggt ccagcggccg ggagtctccc cggtgggtct
     1021 cgaccacggg gcgcggccgg tccgtaggca ggatgaggcg ggccggcgca ccggccagcc
     1081 gctgccgcca atagccgagc tgggcctcca gcacgttccc ctggagccag gctcgctgcc
     1141 acaccgcaaa gtcggcgtac tggaccggcg gctccggcac ctcgagaccg ccatagagtg
     1201 ccgtcaactc gcgcaggaag atgcccatgg accagccgtc ggtgacgatg tggtgcatgg
     1261 tcaccagcag cgcgtggtcg tcgctcgcca gacgcagcag ggccgcgcga accagtggcc
     1321 tcgttcccag gtcgaacggc cgtagcgcct cggccgtggc ccggcacacc agctccgtct
     1381 cgcgtcgaga ctccggcagc cccgacagat ccaacatcgc cagggggagc cgcacagagg
     1441 gggcgatcac ctggatgggc cggccctcgc gctcggcgaa ggcggtgcgg agcgcctcgt
     1501 ggcggcgcgc cacctcctcc agagcctccc cgagcagagc ggggctcaga aggccggaga
     1561 tgcgcaccgc caggggcacg ttgtaggccg ggctccccgg agcgagccgc tccaggaacc
     1621 agagccgctg ctgggcgaag gacaacggca agccgccgtc ttctccgagg gagcgaggga
     1681 tcggtacgat gggcggcgcc ggcaccgtgg actcgcgcag ccgctcgagc cgtccggcca
     1741 gccccgccac cgtcggctcc tcgaacagct cccgcagcgg cagctctact ccgaagacct
     1801 cgcgcacccg cgaggtcacc tgggtggcca gcagcgagtg cccgcccaga tcgaagaagc
     1861 cgttccggac gccgacccgc tcatgccgca gcacctccgc gaagattccc gccagcacct
     1921 cctcggtcag gctgctcggc gctacgtagg tctccagccg gtcgcgctca tgccgcgccg
     1981 gtgcgatcgc cgccagagcc ttgcggtcca gcttgccgtt gggggtcagc ggcagggagt
     2041 cgagccgtac gaagaccggc ggcaccatcg attcgggcag gcggccgcgc agccagcccc
     2101 gcagctcatc cgggcccact tcggagcgcg atgccaggaa cgccaccaga tacggctcgc
     2161 cgcgacgctc ttcccgcacc agcaccgccg cctctcggac ctgcgggtga gccgacagca
     2221 ccgcctcgat ctcccccagc tcgatacgca ggccgcg
//
LOCUS       DASPJI010000787        11116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_450997, whole genome shotgun sequence.
ACCESSION   DASPJI010000787 DASPJI010000000
VERSION     DASPJI010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11116)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11116
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450997"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2268
                     /locus_tag="VEL74_22110"
     CDS             <1..2268
                     /locus_tag="VEL74_22110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65292.1"
                     /translation="AGAGTVDAECRDKHYGGCSPEECLDLLRQLEGASGEEYRKLRED
                     YRQECQPVPVDELIDRSIAERDRYRELRRELEQGELTPEKVVIDPPDLTGVPPRGLSP
                     RQAARLEQVRREAEADYIDLVTAREALLQGGEAVAAAPPPPCDAPAALAGRLTQRRRR
                     ACHDWEADSLRVFACEPDPDGTPAAALCPPRPSEAGRCKQLFSVPAESDLRVMGWVPL
                     WHWETGGGPAEELAVPVAGALLPPLTVWEAAGGEGAWPLDDSAQADLRVVVATSSGQV
                     RVLTPAGGSFPVAESAERLGLPCECRTTGDPLAPLRLTLLARHPETRLLGEPVPGPDG
                     WIGECAVRDVPGGIPDVLCYQARRREAASGGPLPSTVRAWRPLRAAWAGVEAEDALAR
                     RLLAEPAGAGAQALVRHLLAPNPPDSADTPLAPLVVATAAGGEGLPAALLWTGAGLRV
                     LDAAGEARDLEGETGRRLRQLLETPGGEGSPVRRVQLLATGVALAEPGAKLLALRPER
                     GLCYRPAGAAARVLCLRPSSAEAGEAAEPENWQPFAAENPLGEAAAVNRVADAAPDLA
                     RALLEAVLDHSEGRLAVIDVLGEGERRSALLAATGPAGSGAVRLDKVWLAAASGRLSD
                     AVPVVAYGRPEDLPEVYRRHRTGLTELLTLSGAASVRRLLVGPVTRELRRLAVETDGP
                     GGLKVSLWAETGGPFRNVDLDCLRGLVKPYLKPALLEKLGREELLETILSTLPGEDRQ
                     GWEINPFQLLASSGC"
     gene            2296..3621
                     /locus_tag="VEL74_22115"
     CDS             2296..3621
                     /locus_tag="VEL74_22115"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HYG65293.1"
                     /translation="MTRKRDAGALRFFEVPGAYGVAAFLLAGLLTVLTFHGLNVALDR
                     WVDRELDQGIQIKVAQGQSIEEARFEVLHAMAPAIFEWRQPLTAINHAKFLEERFEAS
                     LSGERFSRSSLERVIVSVAQHACWAGNQETCRSLGETGDASPAVPSHLRYLRFLSFRP
                     QAERISDLQRRLEGYELHPGLSLLGLRKAVEPAPFAGRIFRVKRDEPMQERARLETLE
                     SFLDGLTARLAHEAWLPRRLILMLNGEIQWLTLFTGLWCLFLLVWRWRRARKGRWQVA
                     AFRRDGDMTRSSLPALWIDEGRALVGSGLPEPEARAELASRYERALEVRHYSAIDWLI
                     LIVPMLGFIGTIVGMTLAMGNAGQVVAAESQTDLQRAMSNLTGYLGTAFDTTLVALVL
                     SILLEGVRRSVARREFQLVEHLRPIRFDSSEGASDEAAQPRPVAQAGGG"
     gene            3623..4237
                     /locus_tag="VEL74_22120"
     CDS             3623..4237
                     /locus_tag="VEL74_22120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65294.1"
                     /translation="MSGNRGGDHNTFLQDLLSNALAAVIVLTLVVLAISSAAGEQAQT
                     RQRERERSRVQPVRGFRMDQARVDRRDDTLSALLLQLEVQGAGKTPDLQLAGGIAPYG
                     PAGEVMHWRGGEDARHHLFVIPAAPESGGPREWQVRMDGASGPVSLRWRVVADEVVLL
                     AGEQEIEPHGGGPEAVLAVCLGVADEVAVVPAGWNAPTACQGGV"
     gene            4260..4913
                     /locus_tag="VEL74_22125"
     CDS             4260..4913
                     /locus_tag="VEL74_22125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65295.1"
                     /translation="MLDVISNTLGAMLLIFLLVAPKLLDRPLERREETLLVQARAADG
                     QAVVRLWVQPPDSAERFFCDPADPARRDAFELTTVPQVFARAEEGVDRTGAGGNENEE
                     AGAGPPAALWLRPAGPAGNRDRHACAILVRDPAPGCWSYGAVYSDHPQLEQATEIEVG
                     VWSSGAGLIAEGDSSFRPATAEEGAISWQRRSFELHPGTEVTGRIVAAFPGGQAPDC"
     gene            4957..6120
                     /locus_tag="VEL74_22130"
     CDS             4957..6120
                     /locus_tag="VEL74_22130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65296.1"
                     /translation="MRNSVGRSLPWRCMALAVALWSAGAQAAQAQGADKVLLQVQAFH
                     EEMRGSVAALARKVNDLKVELQSTRTAMEGRLDGLRSPVTSIQSSLTSLGAALGELAR
                     ETAAVKALLDQPDEGALALLAGLRTGVAGVEGRVGAVATAVESNRSALADVGSRVAAV
                     EESVQLPAGSDGPLGVFLAGWKEESTTSLDGLRSSSASVGALVLDETQGWQDLRRRVT
                     ALETTSAGIQRELEEQARRQVEHLDRILSGLERIEGGVASGEARIVEFSADAGERGGA
                     PGLEMMVEADAVAAAGERATIEVLVFHGDGRRFVYDGQEGYGDEAGNLMVRESPGAPP
                     DGRVRADLFLPFRVFGDTRPERLELRARLRRADGTVLAGRALLWREMPAASGR"
     gene            6185..6751
                     /locus_tag="VEL74_22135"
     CDS             6185..6751
                     /locus_tag="VEL74_22135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65297.1"
                     /translation="MRWGWVAGTGLMLLVGLSGPAVSQENLLHEFLQCTKEAPVADRS
                     PCNWFTAKAIQRLWGVKDFQRAGKPGEYLTANEIADFVAGSRCWERLGTAADDETLEL
                     AAREANAGLPVVAVYKAQPHGHVALVTRGPVQHSGTWKSNVPMSASFLLDDPQASYVS
                     GPLSKAFGPAKRPAVALYARTRFAPECG"
     gene            6995..7258
                     /locus_tag="VEL74_22140"
     CDS             6995..7258
                     /locus_tag="VEL74_22140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65298.1"
                     /translation="MLTTRHLARTFLRLTLPVAVLAVALLGAPAQADPQTSCVYGFHD
                     QYFDEFGNLCASGDTCSGSKPRGCYLDFSYVSWQQDVILCYCD"
     gene            7371..7832
                     /locus_tag="VEL74_22145"
     CDS             7371..7832
                     /locus_tag="VEL74_22145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65299.1"
                     /translation="MRTFGSIALALALGASPVLAQAPTTRAFTVTSADGKPVNAQADL
                     PAFSPSVVVILVGGTGLRDRDESIGKSGTARDLVFADLARRMNKRGVAAVRYDKRGIR
                     HGALPMDRFDRAAAATATVETMRWNFAHQSECDARASARSGDGDARHRALS"
     gene            7807..8133
                     /locus_tag="VEL74_22150"
     CDS             7807..8133
                     /locus_tag="VEL74_22150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65300.1"
                     /translation="MPGTAPYPDAKTAMAAYSWWKSWFTDDISIATRLAAWQTPMSFH
                     YGTLDSQANADRQRAAAGPIASRARFTIHPDRGHTLGPHVLLGPIDEAIADKIADEVA
                     AVRCGQ"
     gene            8259..8804
                     /locus_tag="VEL74_22155"
     CDS             8259..8804
                     /locus_tag="VEL74_22155"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HYG65301.1"
                     /translation="MPRASWRLLFVLLAGLFAFPSLAQEAPAPPGDPPVSTEAANKAL
                     VRRYVDEVLSAGKLELLDEMVAPEYVDRTPGVPDRGRGPAALRAAQQQMRRLFSQIRY
                     TLESLVAEGDEVAARYTVRAIRPGEGADAGRTVTVTGITLFRIVDGRIRETWIVNDQL
                     GMFQQLGYRLQEPEAKNEPTP"
     gene            8801..9307
                     /locus_tag="VEL74_22160"
     CDS             8801..9307
                     /locus_tag="VEL74_22160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65302.1"
                     /translation="MNARQLLLALLAVPAVLTVLATGCRTAGVPAPEGAPPGMETTAP
                     ALLATREPEVAWRTKSLVKADLDGDGKEDYALGGVKGDRYVLGIVQGPPGSGSRHWTL
                     DFGVAEEDQESLCSLQAQLAVEDLDRKGSEGEMAANAKGLRLHDDRCDAFHIFWNTRE
                     KRYEWWRL"
     gene            9295..>11116
                     /locus_tag="VEL74_22165"
     CDS             9295..>11116
                     /locus_tag="VEL74_22165"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG65303.1"
                     /translation="MAALTEGRGGAGMRELLGGIIDCSRDLIAALDLELRLTALNRAY
                     RQESQRLFGCDLAVGDSLLEALSHRPEDQENARRLWGRALSGESFSEELRFAEPGGGH
                     RFFEAVYTPLRDTAGRVVGACHVVRDVTADRQAEAVRRKLELLDRLEQGTRELSDPVE
                     IMAVVTREVARHLGASRCAYADVESDSDGFTIPQDYSEGVPTSAGDYRLSLFGPRAVA
                     DLRGGRTLVLRDVEGELAPGEGPEMFGELEIRAIICCPLVKEGRLVAMMAVHQTAPRD
                     WTADEVALVETMVERSWAYIERARAQRVLQQAKDAAEEASRAKDQFLAVLSHELRTPL
                     TPVLAAAQLLEMDRDLPARHRDSLAMIRRNVQLEARLIDDLLDLTRIARGKLELQLAS
                     VDVHEKIRLVVRMLEAEAAAKPVSVAVHLEAARPLVTGDAARIQQVLWNLIKNSVKFT
                     PPEGGVQIRTWDSPEGRLRVEVRDTGVGIDPEVLPRIFDAFEQGGSSVTRQFGGLGLG
                     LAISKSLVDLHGGTLTAASEGPGRGATFTLELPALIPDATTAAGAAAAAPAPPENRGA
                     RLLLVEDHLDTADAMARLLGSFGYDVRVAGTVASALEAAES"
BASE COUNT         1622 a         3773 c         4015 g         1706 t
ORIGIN      
        1 gccggcgccg ggacggtgga cgccgaatgc cgtgacaagc actatggcgg ctgcagcccc
       61 gaggagtgcc tcgacctgct gcgccagctc gaaggcgcca gtggggagga gtacagaaaa
      121 ctgcgggagg actaccgcca agagtgccag ccggtaccgg tggacgagtt gatcgaccgg
      181 tctatcgccg agcgcgaccg gtaccgcgag ctgcggcggg agctggagca gggtgagctc
      241 accccggaga aggtggtgat cgatcccccg gatctgaccg gcgtgccgcc gcgcgggctg
      301 tccccccggc aggcggcgcg cctggagcag gtgcgccgcg aggccgaagc ggactacatc
      361 gacctggtga cggcgcggga ggcgttgctc cagggaggag aggcggtggc cgcggccccg
      421 ccgccgccct gcgacgcgcc ggcggcccta gcgggccgcc tcacccagcg ccggcggcgg
      481 gcctgccacg actgggaggc ggactctctc cgcgtgttcg cctgtgagcc cgatccggac
      541 ggcactccgg ccgcggctct ctgcccgccc cggccctccg aggccggtcg ctgcaagcag
      601 ctcttctcgg tgcccgcgga gtcggacctg cgcgtcatgg ggtgggtgcc cctctggcac
      661 tgggagacgg gcggcggccc ggcggaggag ctggcggtac cggtcgcagg agccctgctg
      721 ccgccgttga ctgtctggga ggcggcgggc ggcgaggggg cgtggccgct ggacgattcc
      781 gcccaagccg acctgcgggt cgtcgtcgcg acttcctcgg gacaggtgcg cgtcctgact
      841 ccggcgggtg gaagcttccc cgtcgccgaa tcggcggagc ggctgggtct gccgtgcgaa
      901 tgccggacga caggtgaccc tctcgccccg ctacgcctca cgcttctcgc tcgtcatccc
      961 gagacgcggc tcctcggcga gcccgttccg ggccccgatg gctggatcgg agagtgtgcc
     1021 gtccgcgacg ttccgggcgg cattccggac gtcctctgct atcaggcgcg gcggcgcgag
     1081 gcggcatccg gagggcctct cccctccacg gtccgcgctt ggcggcccct gcgtgccgcc
     1141 tgggctgggg tggaggccga agacgcgctg gcacggcggc tcctcgccga gccggccggc
     1201 gccggcgcac aggccctggt ccgccatctc ctggcgccga accctccaga ctccgcggac
     1261 acccccctgg cgccgctcgt ggtggcgacg gcggccggtg gggaggggct gcctgccgct
     1321 ctgctctgga ccggcgccgg cctgcgcgtc ctcgacgccg cgggcgaggc ccgcgacctc
     1381 gagggcgaga ccggccggcg cttgcggcag ctcctcgaaa ccccgggggg cgagggctca
     1441 ccagtgcgcc gggtccaact cctcgccacc ggcgtcgccc tggccgagcc aggggcgaag
     1501 ctcctcgctt tgcggcccga gcgcgggctc tgttaccgcc cggccggcgc cgccgcccgg
     1561 gtcctctgcc tgcgcccgtc cagtgccgag gccggtgagg ccgccgagcc ggaaaattgg
     1621 caaccgttcg ccgcggagaa cccgctcggt gaagcggcgg ccgtgaatcg ggttgctgac
     1681 gccgcgccgg atctcgcccg cgccctgctg gaggcggtcc tcgatcactc cgaggggcgc
     1741 ctcgccgtga tcgacgtcct cggcgagggg gagcgccggt ccgcgctcct ggccgccact
     1801 ggaccggccg ggagtggcgc cgtccgcctg gacaaggtct ggcttgccgc tgcctccggc
     1861 cggctttctg atgccgtgcc ggtggtcgcg tacggccgcc cggaagacct gccggaggtc
     1921 tatcgccgtc atcgcaccgg gctgaccgag ctcctgaccc tttctggagc cgcttccgta
     1981 cggcgccttc tggtgggtcc ggtgacgcgc gagctccggc ggctcgccgt ggagaccgac
     2041 gggccgggag gtctgaaggt ctcgctctgg gcagagaccg gcgggccctt ccgcaatgtc
     2101 gacctggact gcctccgggg gcttgtcaag ccctacctca agccggccct gctggagaaa
     2161 ctcggcagag aagagctcct ggagacgatc ctctcgacgc tgcccggaga ggaccggcag
     2221 ggctgggaaa tcaatccatt ccaactgctc gcatcctccg gctgctgagg actgcgcacc
     2281 gaaagggaaa gtgtcatgac ccgcaaacga gacgccggcg cgctacgatt cttcgaggtc
     2341 ccgggggcct acggggttgc cgcgttcctc ctggccggcc tgctgaccgt cctgaccttc
     2401 cacggcctca atgtggcgct ggaccgatgg gtggacaggg agttggacca ggggatccag
     2461 atcaaagtgg cacagggtca gtcgatagaa gaggcgcgct tcgaggttct ccatgcgatg
     2521 gctccggcga tcttcgaatg gcggcagcct ctcaccgcca tcaaccacgc gaagttcctg
     2581 gaggaacgct ttgaggcctc cttgtcggga gagcgcttct cccgctcctc tctggagagg
     2641 gtgatcgtct ccgtggccca acatgcctgc tgggctggca atcaggaaac ttgcaggagc
     2701 ctcggggaga caggggatgc ctcccctgcc gtaccctcac atctgcggta tctgcgcttc
     2761 ctcagcttcc gacctcaagc ggagcggatc tccgatctgc agcggcggct cgaaggctat
     2821 gagctccatc caggtctctc actcctcggc ttgcggaagg ccgtggagcc ggctccgttc
     2881 gcgggccgca tcttccgggt caaacgggac gagccgatgc aggaaagggc acggctggag
     2941 acgctggagt cattcctgga cggcctcacc gcgcggctcg cgcacgaggc gtggctcccc
     3001 cggcgcctga tcctgatgct caacggcgag atccaatggc tgacgctgtt caccgggctc
     3061 tggtgccttt tcctgctcgt ctggcgctgg aggcgggcgc ggaagggccg gtggcaggtc
     3121 gcggcgttcc gtagggacgg cgacatgacc cggagcagtc tgccggcgct ctggatcgac
     3181 gaggggcgcg ccctcgtcgg ctctggtctg ccggagccgg aggcccgggc ggagctcgcc
     3241 tcccgctacg agcgggcgtt ggaggtgcgc cattactccg ccatcgactg gctgatcctc
     3301 atcgtgccca tgctggggtt catcggcacg atcgtcggca tgacgctggc catgggcaac
     3361 gccgggcagg tggtggccgc cgagagtcag acggacctgc agcgcgccat gtccaatctc
     3421 accggctatc tcggcaccgc cttcgacacc accctcgtcg ccctggtgct ctccatcctg
     3481 ctggagggcg tgcggcgctc tgtcgctcgg agggagttcc aactggtcga gcacctgcgg
     3541 ccgatccggt tcgactcgtc cgagggggcg agcgacgagg ctgcgcagcc cagaccggtg
     3601 gcgcaggcgg gaggtggcta gcatgtccgg aaaccgaggc ggcgaccaca acacgttcct
     3661 ccaggatctg ctgagcaacg ccctcgccgc cgtgatcgtc ctgaccctgg tggtgctggc
     3721 gatctccagc gcggcagggg aacaagccca gacgcgacag cgcgaacggg agcggtcgag
     3781 ggtgcagccg gtgcggggct tccggatgga ccaggctcgg gtcgaccggc gagacgacac
     3841 tctctcggcc ctgctcctcc agttggaggt ccagggcgca gggaaaacgc cggacctcca
     3901 actcgccggc ggaatcgctc cctacggtcc tgccggtgag gtcatgcact ggcgcggtgg
     3961 ggaggacgct cgccatcatc tcttcgtgat tccggcggcg ccggagagcg gcggtccccg
     4021 ggagtggcag gtgcgcatgg acggcgcttc cggtccggtg tcgctccgct ggcgggtggt
     4081 ggccgacgag gtggtgctgc tcgcgggcga gcaggagatc gagccacacg gcggcggtcc
     4141 cgaggccgtg ctcgccgtct gtctcggagt ggcggacgag gtcgcggtgg tgccggcagg
     4201 ctggaacgcg cccacggcat gccagggagg tgtctgacgt gaaaggcgcc ggcgtcttcg
     4261 tgctcgacgt gatctccaac accctgggag ccatgctcct tatcttcctc ctcgtcgccc
     4321 ccaagctgct cgaccgtccg ctggaacggc gtgaagaaac cctcctcgtg caggcccgtg
     4381 ccgcggacgg ccaggcggtc gtgcggctct gggtacagcc tccggactcg gcggaacgtt
     4441 ttttctgcga tccggccgat ccggcgagaa gggatgcctt cgagctgacc accgtccccc
     4501 aggtcttcgc gcgcgccgag gaaggcgttg acagaacagg ggccggaggc aacgagaacg
     4561 aagaggccgg tgccggccct cccgccgccc tgtggctccg gccggccggc cctgccggca
     4621 accgcgaccg gcacgcatgc gccattctgg tgcgcgaccc ggcaccgggc tgctggagct
     4681 acggcgcggt gtactcggac cacccccaac tcgaacaggc tacggagatc gaagtgggcg
     4741 tctggtcgag cggtgccggc ctgatcgccg agggcgactc ctcgttccga ccggcaacgg
     4801 ccgaggaggg cgccatttcc tggcagagac ggagcttcga gttgcatccc ggcaccgagg
     4861 tgacgggccg gattgttgct gcgttcccgg gggggcaggc gccggattgc tgaggcgcaa
     4921 gccaatgaac cggtgcgttc ggaaaggaga aggaacatga gaaattcagt cggacggagc
     4981 ctgccctggc gctgcatggc gctggccgtg gcgctctgga gcgctggggc ccaggccgcg
     5041 caggcccagg gcgccgacaa ggtgctgcta caggtccagg ccttccacga ggagatgcgc
     5101 gggtcggtgg ccgccctcgc tcgtaaggtc aatgacctca aggtcgaatt gcagagcacg
     5161 cgtaccgcta tggagggtcg gctcgacggg ctcagatctc ccgtgaccag tatccagtca
     5221 tccctcacca gcctcggcgc cgcgcttgga gagctggccc gcgagacggc ggcggtcaag
     5281 gccctgttgg accagccgga cgaaggggca ctggcgctgc tggccggcct tcgcaccggg
     5341 gtggccgggg tcgaaggccg ggtcggtgcc gtcgcgacgg ctgtcgagtc gaaccggtcc
     5401 gccctcgccg atgtcggcag tcgggtagcc gccgtcgagg agagcgtcca gttgccggcg
     5461 gggagcgacg ggcctctagg ggtgtttctc gccggatgga aggaggagag cacgacttcc
     5521 ctggacgggc tgcgatcgtc ctctgcttcc gtaggcgcac tggttctgga cgagacccag
     5581 ggctggcaag acctcaggcg ccgggtgaca gccctggaga cgacgtccgc cgggattcag
     5641 cgagagttgg aagagcaggc gcggcgccag gtcgagcacc tggaccgtat cctgagcggc
     5701 cttgaaagga tcgagggagg agtcgccagc ggggaggcga ggatcgtgga gttctccgct
     5761 gacgctggag agcgtggcgg cgcgccggga ttggagatga tggtggaggc cgatgccgtg
     5821 gcggccgccg gtgaacgagc gaccatcgag gttctggttt tccacggcga cggccggcgc
     5881 ttcgtttatg acgggcaaga gggctacggc gatgaagccg gcaatctgat ggtgcgggag
     5941 agccctggcg cgccgccgga tgggcgggta cgggccgatc tgttcctgcc cttccgcgtt
     6001 ttcggggaca cccgccccga acgtctcgag ttgcgggcgc gcctgcgccg ggccgatggg
     6061 accgtgctgg ccggccgcgc gctcctgtgg cgggagatgc cggccgccag cggcagatga
     6121 ggtgatgaag tcctttccct ttggaatcga ggcagtcatg cggagacgtg caacaggagg
     6181 tgtaatgcgg tggggatggg ttgcaggcac tggcctgatg ttgctggttg ggctgtccgg
     6241 gccggccgtg tcgcaggaaa atctgctcca tgaattcctg cagtgcacaa aagaggcgcc
     6301 ggtggccgat cgatcgccgt gcaattggtt caccgccaag gcgatccaac gtctctgggg
     6361 cgtgaaggac ttccagaggg ctggcaaacc cggcgagtac ctcacggcca atgagatcgc
     6421 cgacttcgtc gctggctccc ggtgctggga acggctggga acggcagccg atgacgagac
     6481 cctggagttg gcagcgcgcg aagccaatgc cggcctacct gttgtcgccg tctacaaggc
     6541 acaaccccat ggccatgtcg cgctcgtcac tcgtgggcca gtgcaacact ccggcacctg
     6601 gaaatccaac gtgccgatgt cggcttcgtt cctgttggac gatccccaag cttcctatgt
     6661 cagcggaccg ctttccaagg cattcggccc ggcgaagcgt ccggcggtgg cgctctatgc
     6721 gcggacgcgg ttcgcgcccg agtgcggcta gatcacggcg gaggcggaca gcggcgcatc
     6781 gagcctttgc ccgtcagggg cgggctgttc caggtgagtc agagcgcggc ccagggaggg
     6841 ggccggcgcc gtcagcctgg agcgcgccgg cgccatgctc gacgagcgcg tctccgaagt
     6901 caccgagtag agtccggcgc caccggcggg cctcccgccg gtggcgctcc atttctctgc
     6961 tacctttctc tccacttttc aaaggagaga gagcatgctc acgacccgcc acctcgcccg
     7021 gacgttcctt cgtctgacgc tgcccgtcgc cgtcctcgcc gtcgccttac tgggcgctcc
     7081 ggcgcaggcc gatccccaga cctcttgcgt ctatggcttc cacgaccagt acttcgacga
     7141 attcggcaat ctctgcgcca gcggcgacac ctgtagcggc tctaaacccc gtggctgcta
     7201 cctagacttc tcgtacgtct cctggcagca ggacgtgatc ctctgctact gcgactgagg
     7261 aggaggcctg ccggggctgt ggaggtttag cgctgaaatg aaatccatgc ctctgacata
     7321 ctgggtgggg gctgctgagc ctcagaacca gaataggaag gaacggattc atgaggacct
     7381 tcggctcgat cgcgttggct ttggcgctgg gcgccagccc ggtgttggcc caggctccca
     7441 ccacgcgcgc cttcaccgtc acatccgcgg atggcaagcc agtcaacgcc caggccgacc
     7501 ttcccgcctt ctccccgtcc gtcgtcgtca tcctggtcgg cgggacgggc ctgcgcgacc
     7561 gtgatgagag catcggcaag tcgggtacgg cgcgcgacct ggtgtttgcc gatctcgcgc
     7621 gacgcatgaa caagcgcggc gtcgccgcgg tgcggtacga caagcgcggc attcgccacg
     7681 gcgcgctgcc gatggacagg ttcgaccgcg ccgccgcagc caccgcgacg gtcgagacca
     7741 tgcgctggaa ctttgcgcac caatcagagt gcgatgctcg agcaagtgcg cgctcaggtg
     7801 atggcgatgc ccggcaccgc gccctatcct gacgccaaaa cggcgatggc ggcctactcc
     7861 tggtggaaga gctggttcac tgatgacata tccattgcca cgcgcctggc ggcttggcag
     7921 acgccgatga gcttccacta cggcacgctc gattcgcagg ccaatgcgga ccgccaacgc
     7981 gccgcggcag gacccattgc ctcacgcgcc cgcttcacga tccaccccga ccgcggccac
     8041 acgctaggcc cccatgtgct gctcggcccg atcgatgagg cgatcgccga caagatcgcg
     8101 gatgaagtcg cggcggtccg ctgcggccag tgacgggcgc cgctgcttcg aagggctgta
     8161 gaatcgagcc catgaaggag gtgggctccc gggagcagcc cgggcaagcg gagcgccttc
     8221 gccggcagct tctcccgctg aagaggccgg atcctccgat gccgagagcc tcgtggcggc
     8281 tgttgttcgt cctgctcgcg gggctcttcg cgtttccgag tctcgcccag gaggctcccg
     8341 ctcctcctgg tgatccaccc gtttccacgg aggcggccaa caaggccctg gtccgccgct
     8401 acgtcgacga ggtgctctcg gccgggaagc tcgagcttct cgacgagatg gtggcgccgg
     8461 agtacgtcga ccgcaccccc ggcgtcccgg accgggggcg cggcccggcg gcgctgcgcg
     8521 cggcgcagca gcagatgcgc cgccttttct cccaaatccg gtacactctc gaatccctcg
     8581 ttgccgaagg ggacgaggtg gcggcccgct acaccgtccg cgcgatccgc cccggagagg
     8641 gtgccgacgc tggacggacg gtgaccgtga ccggcatcac gctcttccgg atcgtggacg
     8701 gtaggatccg ggagacctgg atcgtgaacg accagctcgg catgttccag cagctcggct
     8761 accggctgca ggagcccgaa gcgaagaacg agccaacgcc atgaacgcac gccagctcct
     8821 cctcgccctc cttgccgtcc ctgccgtcct gacggtcctg gccacgggct gccgcaccgc
     8881 cggcgtcccc gctccggagg gggcgccgcc cggcatggag accaccgctc cggcgctcct
     8941 cgccacccgc gagccggagg tggcgtggcg gacgaagagc ctggtcaagg ccgacctcga
     9001 cggcgacggc aaggaggact acgcgctcgg cggggtgaag ggggaccgtt acgtcctcgg
     9061 catcgtccag gggcctccgg gctcgggctc ccgtcactgg actctggact tcggcgtcgc
     9121 cgaggaggac caggagtccc tctgctcgct ccaggcgcag ctcgccgtcg aggatctgga
     9181 ccgtaaaggc agcgaggggg agatggcggc caacgccaag ggcttgaggc tccacgacga
     9241 ccgctgcgac gcctttcata tcttctggaa cactcgcgag aagcgctacg agtggtggcg
     9301 gctctgactg aggggagagg tggcgcgggc atgcgggagc tgctcggcgg gatcatcgac
     9361 tgcagccgcg acctgatcgc cgcgctggac ctcgagctcc gcctcaccgc tctcaaccgt
     9421 gcctaccggc aggagtccca acggctcttc ggctgcgacc tcgcggtcgg cgacagcctg
     9481 ctggaggcgc tgagccaccg gccggaggac caggagaacg cccgccggct ctggggccgc
     9541 gcactgagcg gcgagagctt ctccgaggag ctgcgcttcg ccgagcccgg aggcggccac
     9601 cggttcttcg aggcggttta cacccccctc cgcgacaccg ccggccgggt ggtgggcgcc
     9661 tgccacgtcg tccgcgacgt gaccgccgac cgccaggccg aggccgtgcg ccgcaagctg
     9721 gagctgctcg accggctgga gcaggggacg cgcgagctct ccgatccggt ggagatcatg
     9781 gccgtggtca cgcgcgaggt ggcgcgccat ctcggcgcct cgcggtgcgc ctacgccgac
     9841 gtggagagcg actccgacgg tttcaccatc ccccaggact acagcgaagg ggttccgacg
     9901 agcgccggcg actaccggct ctccctcttc ggaccgcggg cggtggccga cctgcgcggc
     9961 ggccggaccc tggtcctgcg cgacgtggag ggtgagctgg cgccgggcga ggggccggag
    10021 atgttcgggg agctggagat ccgggcgatc atctgctgcc cgctggtcaa ggaggggcgg
    10081 ctggtggcga tgatggccgt gcaccagacg gcgccgcggg actggacggc ggacgaggtg
    10141 gcgctggtcg agaccatggt cgagcgctcg tgggcctaca tcgagcgcgc ccgcgcccag
    10201 cgggtgctgc agcaggccaa ggacgccgcg gaggaggcga gccgcgccaa ggaccagttc
    10261 ctggcggtcc tgagccacga gctgcgcacg ccgctgacgc cggtcctggc cgcggcccag
    10321 ctcctggaga tggaccgcga cctgcccgcc cggcaccgcg actccctggc catgatccgc
    10381 cgcaacgtcc agctcgaggc gcggctgatc gatgacctgc tcgacctgac ccgcatcgcc
    10441 cgaggcaagc tggagctgca gctcgcctcc gtcgacgtcc acgagaagat ccgcttggtg
    10501 gtgcggatgc tggaggccga ggccgcggcc aagccggtga gcgtggcggt ccacctcgag
    10561 gcggcccggc cgctggtcac cggggacgcg gcgcggatcc agcaggtgct ctggaacctg
    10621 atcaagaact cggtcaagtt caccccgccg gagggcggcg tccagatccg cacctgggat
    10681 tcaccggagg ggcggctgcg ggtggaggtc cgggacaccg gcgtcggcat cgacccggag
    10741 gtgctccccc gcatcttcga cgccttcgag caggggggca gctcggtgac ccgccagttc
    10801 ggcggcctcg gtctggggct ggcgatcagc aaatcgctgg tggacctgca cggcggcacc
    10861 ctgacggcgg cgagcgaggg tcccggccgg ggtgcgacgt tcaccctgga gctgccggcg
    10921 ctgatcccgg acgcgacgac cgccgccggc gccgcggccg ccgcgccggc gccgccggag
    10981 aaccggggcg cgcgcctcct gctggtggag gaccacctcg acaccgccga cgccatggcc
    11041 cggctgctgg ggagcttcgg ctacgacgtg cgcgtcgcgg ggaccgtcgc ctcggcattg
    11101 gaggcggcgg agtccg
//
LOCUS       DASPJI010000788         3893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_450998, whole genome shotgun sequence.
ACCESSION   DASPJI010000788 DASPJI010000000
VERSION     DASPJI010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450998"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2625)
                     /locus_tag="VEL74_22170"
     CDS             complement(<1..2625)
                     /locus_tag="VEL74_22170"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYG65304.1"
                     /translation="MEQQQPAPRTIVEVLAERAHRQPDRQVYTFLQDGETETAGLTFG
                     ALDRRTRAIAAALQAHAGSGDRVLLLLPSGPDFITAFLGCLQAGLIAVPAFPPPRRQP
                     DPRLRVIAADAALRVVLTTAALLPKVRPALTGVPGLEGAVWLSVEELAGHDAGRLRET
                     APTGDSLAFLQYTSGSTSSPKGVMVSHGNLLHNERIIQQAFGQTEDSVVVGWLPLHHD
                     MGLIGTVLQPLYTGAHCILMSPVAFLQQPVRWLRAISRYRATTSGGPNFAYDLCTRKV
                     SREQAAELDLSSWSVAFNGAEPVRRETLERFAEAFAPSGFRRQAFYPCYGLAEATLFV
                     AGSASASSDGETRRPVAYGTAWDGQRLAIVDPESGAERAPGEEGEIWIAGPSVAAGYW
                     NRPEETADTFRAMLAGAAGDNGPFLRTGDLGFVDGGELFVTGRLKDLIILRGRNHYPQ
                     DIELTVERSHPALRPGCGASFAVEMDGEERLVVLQEVDRHATGFDAIAEAVRDAVARE
                     HEARVHEVVLLRAGGVLKTSSGKVQRRACRTAYLEGRLPVVVRNVPAGAAAEAEEPGE
                     PWFGLDGLTALPPEQRAAAFAELLRAAAARALSLPLASVDPSRPLTALGLDSLSAAAL
                     EQEVEARTGLRVALPDLLQGISLEGLAADLLAGRGRRPDGGAEDEALAAAPGEESPLS
                     WGQRALWFLQRREPESAAYNLAAAVVIRSQLDVAALRRALEALVTRHASLRVAFGELR
                     GEPFQRLRRGAGLDFRIERAPGAMVEELRPRLRAEAFRPFDLAGEPVLRVRVWETAPD
                     RHVLLWVIHHIAADFTSMTVLLRDLDRLYLAETGGEPASLPPLSWGYPAFVRWQERHL
                     ASAAGERSWRYWRERLDGE"
     gene            complement(2636..>3893)
                     /locus_tag="VEL74_22175"
     CDS             complement(2636..>3893)
                     /locus_tag="VEL74_22175"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYG65305.1"
                     /translation="VQSSKLSLTEALKDEGEKSVGGRGRKRVRRLLVVFETALAFVLV
                     IGAGLLARTFDHLRNVDPGFTPESTLTLQVALPVPKYGDPEVIAFFQRLAEEAAGLPG
                     VRHVGFVNNLPLSGVDVTSSYFVEGQIPAVGEVVPEADLGLISPDYFRALGIPLLQGR
                     HFEPQDGIEGRGAAGGVVIVDEELARRTWPGESPIGKRIKFNRSPDTPWRTVVGMVGH
                     VKYSSLDSSSREQLYVPFARIPSQAAFLVIRTDGDPNTLVGPVRDLVRKLDAELPIFD
                     VQTMTDRLAGSLAKRRLSMSLLLSLAVLALFLAAVGMYSVIAHSVTQRSREIGVRMAL
                     GARRWDIFNLVVGEGLVLALVGIAAGLALAYWATKLLGNLLFGISATDLVTYSMTSLL
                     LIATAAAASYFPARRATRVSPMSARD"
BASE COUNT          586 a         1382 c         1422 g          503 t
ORIGIN      
        1 ctcgccatcg aggcgctccc gccagtaccg ccaggaccgc tcacccgccg cgctggcgag
       61 atgccgctcc tgccagcgga cgaacgcggg gtagccccag gagaggggag gcagcgacgc
      121 gggctcgcct ccggtctcag ccaggtagag ccggtccagg tcgcggagca ggacggtcat
      181 ggaggtgaag tcggcggcga tgtggtggat cacccatagc aggacgtggc ggtccggcgc
      241 cgtctcccag acccgcaccc gcaacaccgg ctcgcccgcg aggtcgaacg gccggaaggc
      301 ctcggcgcgg agccgcggcc ggagctcctc caccatggct ccgggagcgc gctcgatccg
      361 gaagtcgagc cccgcccccc tccgcaggcg ctggaaaggc tccccccgaa gctcgccgaa
      421 cgccacgcgc agcgacgcgt ggcgggtcac cagcgcctcc agcgcgcggc ggagggcggc
      481 gacgtccagc tgcgagcgga tcaccaccgc cgcggcgagg ttgtaggccg cgctctccgg
      541 ctcgcggcgc tggaggaacc agagggcccg ctgcccccag gagagcgggg attcttcgcc
      601 gggagccgcc gccagggcct cgtcttcggc gccgccgtca ggtctccggc cgcggcccgc
      661 caggaggtcc gcggcgagcc cctccaggct gattccctgg agcaggtccg gcagcgccac
      721 gcgcaggccg gtccgcgcct cgacctcctg ctcgagcgcc gcggccgaga gggaatcgag
      781 gccgagagcg gtgagcggcc gggagggatc cacgctcgcc agaggcagcg agagcgcgcg
      841 ggcggcggcg gcgcgcagca gctcggcgaa ggcggcggcc cgctgctccg gcggcagcgc
      901 cgtcaggccg tcgaggccga accagggctc gccgggctcc tccgcttccg cggcggcacc
      961 tgccggaacg ttgcgcacga cgaccggcag acggccctca agataagcgg tgcggcaggc
     1021 gcggcgctgg accttgccgc tcgaggtctt gagcacgccg ccggcacgga gcaggacgac
     1081 ctcgtggacc cgcgcctcgt gctcgcgggc gaccgcgtca cggaccgcct cggcgatggc
     1141 atcgaatccc gtggcatggc ggtccacctc ctggagcacc accagccgct cctcgccatc
     1201 catctccacc gcgaagctgg cgccacagcc gggccgtagc gccgggtggc tgcgttccac
     1261 cgtgagctcg atgtcctggg ggtaatggtt gcggccccgc aggatgatga ggtccttgag
     1321 ccggccggtg acgaacagct cgccgccgtc gacgaacccc aggtcgccgg tgcgcaggaa
     1381 gggcccgttg tctccggcgg cgccggcgag catggcgcgg aaggtgtccg cggtctcctc
     1441 tggccggttc caatagccgg cggcgacgct ggggcccgcg atccagatct ccccctcctc
     1501 gcccggcgcg cgctcggccc cgctctccgg gtcgacgatc gccaggcgct ggccgtccca
     1561 ggcggtgcca taggccaccg ggcgccgggt ttccccgtcg ctggaagcgc tggcagatcc
     1621 ggcgacgaac agggtcgctt cggcgagccc gtaacaggga tagaacgcct ggcgccggaa
     1681 gccactgggg gcgaacgcct cggcgaagcg ttcgagcgtc tcacggcgga ccggctcggc
     1741 gccgttgaaa gcgacgctcc aggagctcag gtcgagctcc gccgcctgct cacggctcac
     1801 cttgcgggta cagaggtcgt aggcgaagtt cgggccgccg ctggtcgtcg cccggtagcg
     1861 cgagatggcg cgcagccagc ggaccggttg ctgcaggaac gccaccggtg acatgaggat
     1921 gcagtgcgcc cccgtgtaga gcggctgcag cacggtgccg atgagcccca tgtcgtggtg
     1981 gagcggcagc cagcccacga cgaccgagtc ctccgtctgg ccgaaggcct gctggatgat
     2041 ccgctcgttg tgaagcaggt tgccgtggct caccatcacg cccttgggcg agctggtcga
     2101 gccggaggtg tattgcagga aggcgagcga gtcgccggtg ggggcggtct cccgcagccg
     2161 gccggcatcg tgcccggcca gctcctccac cgacagccag acggcgccct cgaggcccgg
     2221 caccccggtc agggccggac ggaccttcgg cagcagcgcg gctgtggtca gcacgacccg
     2281 cagcgcggcg tcggcggcga tgacccgcag gcgggggtcg ggctggcgcc gcggcggcgg
     2341 aaaagcgggc acggcgatca ggccagcctg gagacagccg aggaaggcgg tgatgaagtc
     2401 gggcccggag ggcagcagca ggagcacccg gtcgccgctc ccggcgtggg cctgcagggc
     2461 ggcggcgatg gcgcgcgtcc ggcggtcgag cgcgccgaag gtcaggccgg ccgtttcggt
     2521 ctcgccgtcc tgcaggaacg tgtagacctg ccggtcgggc tgacggtgcg cgcgctccgc
     2581 gagcacttcg acgatcgtgc gcggggccgg ctgctgctgt tccatccgtt ggctcctaat
     2641 cccgggctga catcgggctc acccgggtgg cccttctggc ggggaaatag ctcgccgccg
     2701 ccgccgtcgc gatcaacaag agagacgtca tcgaataggt cacgaggtcg gtggcgctga
     2761 tcccgaagag cagattgccg aggagcttcg tcgcccagta ggccagggcc aggccggccg
     2821 cgatcccaac cagcgccagg acgagccctt cgccgaccac caggttgaag atgtcccatc
     2881 gccgggcacc gagggccatg cgcacgccga tctcgcggct gcgctgggtc accgaatggg
     2941 cgatgacgct gtacataccg accgccgcca ggaagagggc caggaccgcc aggctcagca
     3001 gcagggacat ggagagccgg cgcttggcca gcgagccggc gagccggtcg gtcatcgtct
     3061 ggacgtcgaa gatcggcagc tccgcgtcga gcttcctgac caggtcgcgg accggaccga
     3121 ccagagtgtt gggatccccg tcggttcgga tgaccaggaa ggccgcctgg gacgggatcc
     3181 gcgcgaaggg tacgtagagc tgctcccgcg aggacgagtc caggctggag tacttgacgt
     3241 ggccgaccat accgaccacg gtccgccagg gggtatccgg gctccggttg aacttgatcc
     3301 gcttgccgat cgggctctcg ccgggccagg tgcggcgggc gagctcctcg tcgacgatca
     3361 ccacgccacc cgccgcgccg cgcccttcga taccgtcctg gggctcgaaa tgcctcccct
     3421 ggagcagggg gatcccgagg gctcggaagt agtccgggct gatgagcccc agatcagctt
     3481 ccgggaccac ttcgcccacg gccggtatct gcccctcgac gaaataggag ctggtcacgt
     3541 cgacgccgct cagcggcagg ttgttgacga agccgacatg gcgaaccccg ggcaggccgg
     3601 ccgcctcctc cgcgagccgc tgaaagaagg cgatcacctc cgggtccccg tacttgggca
     3661 ccggcagggc gacctgcaag gtcagcgtgc tctccggagt gaagccggga tcgacgttcc
     3721 ggaggtggtc gaacgtgcgg gccagcaggc cggcgccgat gaccagcacg aaggcgagcg
     3781 ccgtctcgaa caccaccagc aggcggcgga cccgcttccg accgcgtccg cccaccgatt
     3841 tctccccctc gtccttgagc gcttcggtca ggctcagctt cgagctctgc acc
//
LOCUS       DASPJI010000789         4343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_222570, whole genome shotgun sequence.
ACCESSION   DASPJI010000789 DASPJI010000000
VERSION     DASPJI010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222570"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VEL74_22180"
     CDS             complement(<1..543)
                     /locus_tag="VEL74_22180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HYG65306.1"
                     /translation="MSESADPRAADLLYRGFPDFAEWQGLTPEDVALWDRFAARLEER
                     RREATPESLRRALDVVIRAAALDTGAIEGLYSVDRGFTMTVAVQGLAWEHAIEERGAG
                     VRAFFEAQLEAYELVLDAVTRSLPVTEVWIRALHEKLCAPQERYRVLTEVGWQEQALP
                     KGEYKNRPNHVRLTDGSFHAY"
     gene            647..850
                     /locus_tag="VEL74_22185"
     CDS             647..850
                     /locus_tag="VEL74_22185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65307.1"
                     /translation="MPGQGSAGNVIAALCSFFIPGLGQLLQGRVLMAIVHFVLAAVLW
                     IFLLGWAIHLWSIIDAAIWKPKS"
     gene            861..1940
                     /locus_tag="VEL74_22190"
     CDS             861..1940
                     /locus_tag="VEL74_22190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65308.1"
                     /translation="MTDTNETETEQQHPDAETLARYTRGELSRDESRELERHLMTCID
                     CQSQVDQTASAGVQWKAHRFGKNLKKTRKAQEEQARRDQAEEERRDQLRGVIRSLSSI
                     IGNLGEQEARELLAESQVGRRKLLQRQDRFHTAHLCELLLERCRRAWLEQPDAAVELA
                     KLAALIAGRLSFEHYGARQVRNLKSMAWMHVGNSFRVARAADIAVAEDGEEWETGEME
                     EEEYPEIETPEYAVAEPSGDTPEVSPKATAEAEAAFFEVRDLFLERGMGFDAALVTME
                     LAAVYQRQGHGDFIPSLAAEAIPLFEQAEERGVPSYTTDALRFLRDTAAAGKLTPEGL
                     TKMHTLLQRRRNDPEFRFKDKEKAP"
     gene            complement(1915..2544)
                     /locus_tag="VEL74_22195"
     CDS             complement(1915..2544)
                     /locus_tag="VEL74_22195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65309.1"
                     /translation="MTKGMKTTGMKRVLTVPVLIAACLGLSCSAHRQAGTGDVAFRLL
                     WNGMSDLDLFVEDPAGGCIYFAQPESGSGGFLDVDCNAGTDQICDQPIENVFWPPGTA
                     PAGRYVFWVYTNSLLAAETPLAYELQVLRGPRVAWRHQGSIQRFGQSSGPLAYDFSRE
                     RDQPPVASDRPLPDCGGWVRQRGNWQPGEVPAPVPPTVPGPSTAPSPCP"
     gene            2701..3270
                     /locus_tag="VEL74_22200"
     CDS             2701..3270
                     /locus_tag="VEL74_22200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65310.1"
                     /translation="MSKRLLLPLLLITVGTGCQTTGSSGPASRVAYFPQLRPTPAEAE
                     LSQSQSGGGVEGTLTLAGGCLRLANGEGDYLVIWPSRVRLDHVGGGIRVIDETSGRMA
                     RLGGTVRLAGSPIETPSPILSELRQPLPACSGPMLLATGITQGDDLPRNSQRLAADGR
                     RRGAPDTGDAGDAGDIAGIAKTIRTPGLR"
     gene            complement(3361..>4343)
                     /locus_tag="VEL74_22205"
     CDS             complement(3361..>4343)
                     /locus_tag="VEL74_22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017219505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HYG65311.1"
                     /translation="LLGNMWAQSWDNIYDLVAPPQADPGFDLTERLKAKNLDHLEMVR
                     YGERFFTSLGFEPLPETFWERSLFVQPEDRNVVCHASAWNIDYKDDLRIKMCIQVNGE
                     DFRTIHHELGHNFYQRAYNQLPPLYQDSANDGFHEAVGDTIALSITPDYLKQVGLIDT
                     VPPASADLGLLMRQALDKVAFLPFGLLIDQWRWKVFSGEIKPTEYNKGWWDLRERYQG
                     VTAPVARTEEHFDPGAKYHVPGNTPYTRYFLAHILQFQLHRGLCEAAGNKAAGERLAN
                     MLEMGASRPWPEALKAATGQEQMDATAILDYFAPLKTWLDQQNKGRKCGW"
BASE COUNT          665 a         1560 c         1454 g          664 t
ORIGIN      
        1 gtaggcgtgg aacgagccgt ccgtgagccg cacgtggttc gggcgattct tgtactcccc
       61 tttgggcagc gcctgctcct gccaccccac ctcggtcagc acccggtatc tctcctgcgg
      121 cgcgcacagc ttctcgtgga gggcccggat ccagacctcg gtgaccggca ggctgcgggt
      181 cacggcgtcc aggaccagct cgtaggcttc gagctgcgcc tcgaagaagg cgcgcacgcc
      241 cgccccccgc tcctcgatgg cgtgctccca ggcgagaccc tggacggcca cggtcatcgt
      301 gaagccccgg tcgacgctgt agagcccctc gatggccccg gtgtcgaggg cggcggcgcg
      361 gatgaccacg tcgagcgctc gccttaacga ttccggcgtc gcctcccgcc gccgctcctc
      421 cagccgggcg gcgaaccggt cccagagcgc cacgtcctcg ggggtgagcc cctgccactc
      481 cgcgaagtcc gggaaccccc ggtagagaag gtccgccgcc cgcggatccg ctgattcgct
      541 caaggtgtct cctccgtcca ccgctgagta tacgagttct cccccttcac cgcccccttg
      601 ggttctgata gacttctccc ccgccgcaac ttcctctgga ggagagatgc ctggccaagg
      661 gtccgcaggg aacgtaatcg ccgcgctgtg cagcttcttc atccccggtc tgggacaact
      721 gctccagggc cgggtgctca tggccatcgt gcacttcgtg ctggccgccg tgctctggat
      781 cttcctgctg ggctgggcca tccacctctg gtccatcatc gacgcggcca tctggaagcc
      841 caaatcctga cgacgtcacc atgaccgata cgaacgagac cgagaccgag cagcagcacc
      901 cggacgccga gaccctcgcc cgctataccc gcggcgagct gtcccgcgac gagagccgtg
      961 agctggagcg gcacctgatg acctgcatcg actgccagag ccaggtcgac cagactgcca
     1021 gcgccggcgt gcaatggaag gcccaccgct tcggcaagaa cctgaagaag acccgcaagg
     1081 cccaggagga gcaggcgcgg cgcgaccagg cggaggagga gcgccgggac caactccgcg
     1141 gcgtcatccg ctcgctgagc agcatcatcg gcaacctggg cgagcaggag gcgcgcgagc
     1201 tgctcgccga gtcccaggtc ggacgccgga agctgctcca gcgccaggac cgcttccaca
     1261 ccgcccacct gtgcgagctg ctgctcgaac gctgccgccg cgcctggctc gagcagccgg
     1321 acgccgccgt ggagctggcc aagctcgccg ccctcatcgc cggccggctc tccttcgagc
     1381 actacggcgc gcggcaggtg cgcaacctca agtccatggc ctggatgcac gtcggcaact
     1441 ccttccgcgt cgcccgcgct gccgacatcg cggttgcgga ggacggagag gagtgggaga
     1501 ccggggagat ggaggaggag gagtacccgg agatcgagac cccggagtac gcggtggccg
     1561 agccgtccgg cgacacgccg gaggtctccc cgaaggccac cgccgaagcc gaggccgcct
     1621 tcttcgaggt ccgcgacctc ttcctcgagc gcggcatggg cttcgacgcg gctctggtga
     1681 cgatggagct ggcggcggtc tatcagcggc aggggcacgg cgacttcatc cccagcctcg
     1741 ccgccgaggc gatccccctc ttcgagcagg ccgaggagcg cggcgtcccc tcctacacca
     1801 ccgacgccct ccgcttcctg cgcgacaccg ccgcagccgg caagctcacc cccgaggggc
     1861 tcaccaagat gcacaccctg ctgcagcggc ggcgcaacga ccccgagttc cggttcaagg
     1921 acaaggagaa ggcgccgtag agggacccgg caccgtcggc ggcacgggcg ccggcacctc
     1981 tcccggctgc cagttgccgc gctggcgcac ccacccgccg cagtcgggga gggggcggtc
     2041 gctcgccacc ggcggctgat cccgctcgcg cgagaagtcg taggcgagcg gtccggagct
     2101 ctgcccgaac ctctggatgg agccctggtg ccgccaggcc acccgcgggc cgcgcagcac
     2161 ctgcagctcg taggcaagcg gcgtctccgc cgcgagcagc gagttggtgt agacccagaa
     2221 gacgtagcgg cccgccggcg cggtgcccgg cggccagaag acgttctcga tcggctggtc
     2281 gcagatctgg tccgtgccgg cgttgcagtc gacgtccagg aagccgccgc tccccgactc
     2341 cggctgcgcg aagtagatgc agccgcctgc cgggtcctcc acgaagaggt cgaggtcgga
     2401 catgccgttc cacagcagac ggaacgccac gtcccccgtg cccgcctgcc ggtgcgccga
     2461 gcaggagagc cccaggcagg cggcgatcag caccggaacc gtcagcaccc tcttcatccc
     2521 cgtcgtcttc atccccttcg tcatggctcc ctccctctct cccccttgag acggcccgaa
     2581 cgcgccgaag gttcccggac gggattctct gctacactct ggcacacctc gtgcagtaca
     2641 gggtctgtgt cacgacttct tccgcgatgt tgacggagga aaagcacagg agattccaaa
     2701 gtgtcaaagc gactgctgct tccgctgctg ctgatcactg taggaacagg gtgccagacg
     2761 acgggctcct cgggtccggc gtcgcgggtc gcctacttcc cgcagctccg gcccaccccg
     2821 gcggaggccg agctgtcgca gtcccagagc ggagggggcg tcgaagggac cctgacgctg
     2881 gccggcggct gcctgcggtt ggccaacggc gagggtgact acctggtgat ctggccgtcc
     2941 cgggtgcgcc tcgaccatgt cggcggcggc atccgggtga tcgacgagac gagcggccgg
     3001 atggcccgtc tcggcggcac ggtgcggctg gccggcagcc cgatcgagac gccgtcgccg
     3061 atcctgagcg agctccgcca gcccctcccc gcctgctccg gcccgatgct gctcgccacc
     3121 ggcatcaccc agggcgacga cctgccgcgc aacagccagc ggctggccgc ggacggccgg
     3181 cgccgcggcg cccccgacac cggcgatgcc ggcgatgccg gcgacattgc cggcatcgcc
     3241 aagacgatcc gtactcccgg gctccgctag tccgccagca ggaggttccg actcacctag
     3301 gaaaagggcc ggccccctcg ggggggaccg gcccttttct cttcagccag gtgccgcgtc
     3361 ctaccagccg cacttccttc ccttgttctg ctggtcgagc caggtcttca agggggcgaa
     3421 gtagtcgagg atggcggtgg cgtccatctg ctcctggccg gtggcggcct tcagcgcctc
     3481 gggccaggga cggctcgcgc ccatctccag catgttggcc agacgctcgc ccgcggcctt
     3541 gttgccggcc gcctcgcaca gcccgcggtg gagctggaac tgcaggatgt gggcgaggaa
     3601 gtagcgagtg tagggcgtgt tgcccggcac gtgatacttc gcgcccggat cgaaatgctc
     3661 ctccgtgcgc gccaccggcg ccgtcacgcc ctggtacctc tcgcgcaggt cccaccaccc
     3721 cttgttgtac tcggtcggct tgatctcccc cgagaacacc ttccagcgcc actggtcgat
     3781 gagcaggccg aagggcagga aggccacctt gtccagcgcc tgccgcatca gcaggcccag
     3841 gtccgcggac gccgggggga ccgtgtcgat caggccgacc tgcttcaggt agtcgggcgt
     3901 gatcgacagc gcgatggtgt cgcccaccgc ctcgtggaag ccgtcgttgg cgctgtcctg
     3961 gtagagcggg gggagctggt tgtaggcgcg ctggtagaag ttgtggccca gctcgtggtg
     4021 gatggtgcgg aaatcctcac cgttgacctg gatgcacatc ttgatccgca ggtcgtcctt
     4081 gtagtcgatg ttccacgcgc tggcgtggca gaccacgttg cggtcctccg gctggacgaa
     4141 gagggagcgc tcccagaagg tctcgggcag cggctcgaag ccgagggagg tgaagaaccg
     4201 ctcgccgtag cgcaccatct ccaggtgatc caggttcttc gccttgagcc gctcggtcag
     4261 gtcgaagccg gggtcggcct ggggcggggc caccaggtcg tagatgttgt cccagctctg
     4321 cgcccacatg ttgccgagga ggt
//
LOCUS       DASPJI010000790         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_222804, whole genome shotgun sequence.
ACCESSION   DASPJI010000790 DASPJI010000000
VERSION     DASPJI010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222804"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2478)
                     /locus_tag="VEL74_22210"
     CDS             complement(<1..>2478)
                     /locus_tag="VEL74_22210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65312.1"
                     /translation="LLAGFAALLRRVTGQGDLVVGTATANRGHAAAEGLIGFFVNSLP
                     LRVDAAGDPAWKGLLGRVRETTLAAYAHQEMPFARLVEELSPERSLAQTPLFQVALVL
                     QNLPVRAAGLPGLSLVPVPVDTGTAKHDWMLTFGEESDGLAGTFEYATELFDAPTIER
                     CLGAFERLLGEVAADPGRRISGLDLLSPAERAQVVREWNDTAPVGVDEAVQMAFARQA
                     RNDPAAPALQYRGLSWSYGELEARADRLAGHLWNLGVGSSSLVAVCLRRSPAAMAAIL
                     GVLRAGGAFLPMDPDDPAERLGRVLADSGASLLLTEAGLAGALPPHGAKTVLVEEMLE
                     TPASPVPPEIPDPLHLAYVIYTSGSTGQPKGVAVTHGGLSNMVRAQVGLFGVEASDRV
                     LQFAAASFDASVSEIFMALTSGACLVLEDREALRPGPGLAAWLEAEAISVLTLTPPAL
                     AVLPPAELPALRTLIVAGEACPPELADRWGMGRRLWNAYGPTEGTVCATAVLHLPGRD
                     RLPIGRPIDGVHVHLVDEGGEPVPVGVTGELALGGAGVARGYLGRPDLTAERFVPDSF
                     GEQPGGRLYRTGDLGRLLSTGEIEFLGRRDTQVKIRGHRVEPGEVEAVLAAHPAVEES
                     AVVVRDDGPAGRRLVACVTAATQALGLRQELRAYLEARLPSHMVPAEIAVLPGLPRTS
                     AGKVDRRALTDLLTGGAGAPGDAGDGGGWPPANPLEELVAGIFAEVLGRDRVGADGQF
                     FDLGGHSLMATQVMSRVEAVLGVELPLQALFEAPTVAGLAERVAAAGPGRRRPPAIER
                     LPRPRKGEMPLSFGQQRLWLIDQLEPG"
BASE COUNT          378 a          942 c          871 g          287 t
ORIGIN      
        1 atccgggctc gagctgatcg atgagccaga gccgctgctg tccgaaggag aggggcatct
       61 cacccttgcg gggccgtggc agccgctcga tcgccggagg ccgccgccgg cccggaccgg
      121 ccgcggcgac ccgctccgcc agacccgcca cggtgggcgc ctcgaaaagg gcctggagag
      181 gcaactccac ccccagcacg gcctcgaccc gcgacatcac ctgggtcgcc atgagggagt
      241 gcccgccgag gtcgaaaaat tggccgtccg cccccacgcg gtcgcggccc agcacctcgg
      301 cgaagatgcc cgcgaccagc tcctccaggg gattggccgg gggccagccc cctccgtctc
      361 cggcatcccc gggggcgcct gcccctcccg tcaggaggtc cgtcagcgcc cggcgatcga
      421 ccttgccggc agaggtcctg ggcagtccgg gcaggaccgc gatctcggca ggcaccatat
      481 gagaaggtag gcgcgcctcc aggtacgccc gcagctcctg ccgcagaccg agcgcctgcg
      541 tcgcggccgt gacgcaggcg acgagccgcc ggcccgccgg cccgtcgtcg cggaccacca
      601 ccgcggactc ctccacggcc ggatgcgccg ccagcaccgc ctcgacctcc ccgggctcga
      661 cgcgatgacc gcggatcttc acctgggtat cgcgccgccc gaggaattcg atctcgcccg
      721 tggacagaag gcgtcccagg tcgccggtgc ggtagagccg gccgcccggc tgctcgccga
      781 aagaatcggg cacgaagcgc tccgccgtga ggtccggccg ccccaggtac ccgcgagcca
      841 ccccggcgcc gcccagggcc agctccccgg tcaccccgac gggcaccggc tctccccctt
      901 catctacaag gtggacatga acaccgtcga tcggccgtcc gatgggcagc cggtcccgtc
      961 cgggtaggtg caaaaccgcg gtggcgcaga ccgtgccctc ggtcgggccg taggcgttcc
     1021 atagccgccg tcccattccc cagcggtcgg ccagctcggg cggacaggcc tcgccggcga
     1081 cgatcagagt ccgcagcgct ggcagctcgg ccggcggcag caccgccagg gccggcggcg
     1141 tcagggtcag gacggagatc gcctccgctt ccagccaggc ggcgaggccg gggccgggcc
     1201 tcagcgcctc gcggtcctcc agcaccaggc aggcgcccga cgtcagcgcc atgaagatct
     1261 cggagacgga ggcgtcgaag ctcgcggcgg cgaattgaag gacccggtcg ctggcctcca
     1321 cgccgaagag ccccacctgc gcccggacca tgttggacag gccgccgtgg gtcaccgcca
     1381 cgcccttggg ttggccggtg gagccggagg tatagatgac gtaggcaaga tggagagggt
     1441 ccggaatctc cgggggtacc ggactcgccg gggtctccag catctcctcg acgaggaccg
     1501 tcttcgcacc gtgcggagga agggcaccgg ccagcccggc ctcggtgagg agcagggacg
     1561 cgcccgagtc cgccagcacc cgcccgaggc gctcggccgg gtcgtccggg tccatgggaa
     1621 ggaaggcgcc gccggcccgg agcactccga ggatcgcggc catcgccgcc ggtgagcgcc
     1681 ggaggcagac cgccaccagc gagctcgagc cgactcccag attccacagg tggcccgcca
     1741 ggcgatccgc ccgggcctcc agctcgccat agctccaaga gagaccacgg tactggaggg
     1801 ccggagccgc cggatcgttg cgggcctgcc gcgcgaaagc catctgcacg gcttcatcaa
     1861 caccgaccgg cgccgtatcg ttccactcgc ggaccacctg agcccgctcc gccgggctca
     1921 gcaggtccag ccccgagatc cggcggccgg gatcggcggc cacctccccg aggagccgct
     1981 cgaaggcccc gaggcagcgc tcgatcgtcg gggcgtcgaa cagctcggtg gcgtactcga
     2041 aggtgccggc gagtccgtcc gactcctcgc cgaaggtgag catccagtcg tgcttggccg
     2101 tgccggtgtc cacgggcacc ggcaccagcg acaggccggg cagcccggcg gcgcggaccg
     2161 gcaggttctg gagcaccagc gccacctgga agagcggcgt ctgagccagg ctgcgctcgg
     2221 gggacagctc ctccaccaga cgggcgaacg gcatctcctg gtgggcatag gccgcgaggg
     2281 tggtctccct cacccggccg aggagccctt tccaggcggg gtccccggcg gcatccaccc
     2341 gcaacggcaa gctgttgacg aagaagccga tgagcccctc ggccgccgcg tgcccccggt
     2401 tggccgtggc cgtgcccacc accaggtcgc cctgcccggt gacccgccgc agcagggcgg
     2461 cgaagccggc gaggagga
//
LOCUS       DASPJI010000791         3189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_619256, whole genome shotgun sequence.
ACCESSION   DASPJI010000791 DASPJI010000000
VERSION     DASPJI010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619256"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VEL74_22215"
     CDS             complement(<1..201)
                     /locus_tag="VEL74_22215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HYG65313.1"
                     /translation="MMDDDTPLAREAIAIVGMSGRFPGADDLDAFWRNLRDGVESIST
                     LSEAELAGAGVEPALAARPDYVR"
     gene            complement(215..>3189)
                     /locus_tag="VEL74_22220"
     CDS             complement(215..>3189)
                     /locus_tag="VEL74_22220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65314.1"
                     /translation="GDAGAVDFAVLAPAPGRLEAEAWRPFALERGPVMRVRLFSSPGE
                     TPVLLLAVHHIAADFWSLGLLLEDLARLVTPREPAEDVPPAPAVSYADWTRWQRELLA
                     GPEGERLWAEWQDALAGAPAVLDLPADRPRPPVPTHRGVSHAFSLGPELSDNVRALAA
                     ASGSTLYTVLLAALAALLHRHSGREDLLVGTGAAGRTRPGLEEVAGHFVSLLPVRIGL
                     AGGPAFAELLARARRSVVGALERQDFPFPLLVERLAPVRDPARHPLVQAAFVLEQPHR
                     LQGGSAAPFVLGRAGAPLTVGDQTGGLVLEPVELPRPATPFDLTLVAVETGGTVEAAF
                     ELGADLFDAPTAARLAGRFLTLLAAAVADPSRRLDELPALTGAERSQLLVEWNDTAGP
                     VPERCAHELFEDWADRTPEAPALIGVDGESETYAELEARANRLANHLRSMGIGPEAPV
                     ALRLERSPDLWMAALATLKAGGYYVPVDPSWPRERIDLVLGDCGARAVVTAGTLDGLS
                     SCSATRPAAGTLPDSTAYGLYTSGSTGRPKGVLVPHRPLVNLVHHGARDLRVGPGSRV
                     LQAGAPVFDASVLEFFLALTSGAALCLVSEEARLSGPRLAAEMRERGVTFSLLTPAAV
                     RFLPEDEVPALATLMVGGEAMPASLATRWAGRLRLFNAYGPAEAAVYAATHRCRPEEE
                     TPPIGRPIPNARLYVLAPWSREPLPPGVAGELHIGGVPPARGYLGRPGLTAERWIPDP
                     FGAPGDRLYRTGDRSRWRPDGRLDFLGRIDSQIKLRGVRIEPGEIEAALALHPEVKDA
                     VVAVRPGAAGEPVLAAYVVPRGESGELAGDLRPFLAERLPAAMVPAAFVTLQAMPVTA
                     VGKVDRGALPPLPSLSSSTSGAADSFTALPRTEMERTIATVWREVLGVEEVGVDRNFF
                     ELGGHSLLLARVHARLCEALGKSIPLGEMFSHTTVRSLAAHLAGEREEEREGAREIEG
                     RAASRRAAMDRRRARSRI"
BASE COUNT          474 a         1205 c         1159 g          351 t
ORIGIN      
        1 ccgcacgtag tccggacgcg ccgccagggc gggctcgacc ccggcaccgg ccagctcggc
       61 ctccgagagg gtggagatcg actcgacgcc gtcgcgcaga tttcgccaga aagcgtccag
      121 gtcgtccgcg cccgggaacc ggccgctcat gccgacgatg gcgatagcct cgcgggccaa
      181 gggggtgtcg tcatccatca tgagaaaacc tcgatcagat cctcgaacgc gcccgccggc
      241 ggtccatcgc ggcgcggcgg ctggcggccc ggccctcgat ttcgcgcgcg ccctcccgct
      301 cctcctcccg ctcccccgcc aggtgcgcgg ccagggacct cacggtcgta tggctgaaca
      361 tctcacccaa cggaatcgat ttcccgagcg cctcgcacag ccgagcatgg acccgcgcca
      421 gcagcagcga atggccaccg agctcgaaga agttgcggtc cacgccgacc tcctcgacgc
      481 ccaacacctc ccgccagacc gtcgcgatcg tcctctccat ctcagtgcgc ggaagagccg
      541 tgaatgagtc ggcggccccg gatgtcgatg aagagaggga ggggagaggg ggcagcgcgc
      601 cgcggtcgac cttgccgacc gcggtcaccg gcatcgcctg tagcgtcacg aaagcggcgg
      661 ggaccatggc ggccggcagg cgctcagcca ggaacgggcg cagatcgccg gcgagctctc
      721 ccgactcgcc gcgcggcacc acgtacgccg ccagcaccgg ctcccccgcc gcgcctggac
      781 ggaccgcgac caccgcgtcc ttgacctccg gatggagggc cagggctgcc tcgatctcgc
      841 cgggctcgat ccgtaccccg cgcagcttga tttgggaatc gatccggccc aggaagtcca
      901 gccggccatc cggccgccag cggctccggt cgccggtgcg gtagaggcgg tccccgggtg
      961 cgccgaacgg gtccgggatc cagcgctcgg ccgtcaggcc ggggcggccg agatagccgc
     1021 gggccggcgg cacacctccg atgtgcagct ctcccgcgac tcccgggggc agaggctccc
     1081 gggaccaggg ggcgaggacg tacaggcgcg cgttggggat cggccggccg atgggcggcg
     1141 tctcctcttc cggccggcag cggtgcgtgg cggcgtagac cgcggcctcg gccgggccgt
     1201 aggcgttgaa cagccggagg cgcccggccc agcgggtcgc cagagaggcc ggcatcgcct
     1261 cgccgccgac catcagcgtc gcgagcgccg ggacctcgtc ctccggcagg aagcggacgg
     1321 ccgcgggggt gaggagggag aaggtgacgc ccctctcacg catctcggcg gccagacgcg
     1381 gtccggacag gcgtgcctcc tcgctcacca gacagagcgc ggcccccgag gtgagcgcca
     1441 ggaagaattc cagcaccgag gcgtcgaaga ccggcgcgcc cgcctgcagc acccgcgagc
     1501 cggggccgac ccggaggtcg cgggccccat ggtggaccag gttgaccagc ggccggtgcg
     1561 gtaccagcac ccccttgggc cggccggtcg agcccgaggt atagaggccg taggccgtac
     1621 tgtccggcag ggtgcccgcc gcgggccggg tggcactgca ggacgagagc ccgtccagcg
     1681 taccggccgt caccaccgcc cgtgcgccgc agtcgccgag caccagatcg atccgctccc
     1741 gcggccacga agggtcgacc ggcacgtagt agccaccggc cttgagggtg gcgagcgccg
     1801 ccatccacag atccggcgag cgctccagcc gcaacgcgac cggcgcctcc gggccgatcc
     1861 ccatggaccg caggtgattg gcgagccggt tggcccgcgc ctcgagctct gcgtacgtct
     1921 cggactcgcc gtccactccg atcagggccg gagcctcggg ggtgcggtcg gcccagtcct
     1981 cgaacagctc gtgcgcgcac cgttccggaa ccggcccggc ggtatcgttc cactccacca
     2041 ggagctggct gcgctcggcg ccggtcagtg ccggcagctc gtcgagccgg cgcgacggat
     2101 ccgccaccgc ggcggcgagc agggtgagga agcggccggc caggcgcgcg gcggtcggag
     2161 cgtcgaagag gtcggcgccg agctcgaagg ccgcctccac ggtgccgccg gtctcgaccg
     2221 ccacgagggt gaggtcgaac ggcgtcgccg gccgcggcag ctcgaccggc tccaggacga
     2281 ggccgcccgt ctggtctccg acagtcagcg gcgcgccggc gcggccgagc acgaacggcg
     2341 ccgcgctccc cccttgcaac cggtgtggct gctccagcac gaacgcggcc tggacgagtg
     2401 gatggcgcgc cgggtcccgc accggagcga ggcgctcgac cagcaacggg aacgggaagt
     2461 cctgccgctc cagggccccc accaccgagc gccgtgcccg tgccagcagc tccgcgaagg
     2521 ccggaccgcc cgcgaggccg atgcgcaccg gcagcaggct cacgaaatga ccggccacct
     2581 cctccaaccc cggccgggta cggcccgccg ccccggtgcc gacgagcagg tcctcccggc
     2641 cgctgtgccg gtggagcaag gcggccaggg ccgcgagcag cacggtatag agcgttgacc
     2701 ccgacgcggc ggcgagggcc cgcacgttgt ccgacagctc ggggccgagc gagaacgcat
     2761 gcgagacgcc gcgatgggtc ggcaccggcg gcctcggacg gtcggcgggc aggtcgagca
     2821 ccgcgggcgc gccggccagc gcgtcctgcc actccgccca caggcgctcg ccttcggggc
     2881 cggcgaggag ctcgcgctgc cagcgggtcc aatctgcata ggagaccgcg ggcgccggcg
     2941 ggacgtcctc cgctggttcc cttggcgtga cgagacgggc cagatcctcg agcagcagcc
     3001 ccaacgacca gaagtcggcg gcgatgtgat ggaccgcgag cagcagcacc ggagtctcgc
     3061 cgggcgagct gaacagccgc acgcgcatca ccggcccgcg ctccagggcg aacgggcgcc
     3121 aggcctccgc ttccaaccgt ccgggcgccg gcgcgagcac cgcgaagtcg accgcgccgg
     3181 cgtccccga
//
LOCUS       DASPJI010000792        14924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_160259, whole genome shotgun sequence.
ACCESSION   DASPJI010000792 DASPJI010000000
VERSION     DASPJI010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14924)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14924
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160259"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VEL74_22225"
     CDS             <1..186
                     /locus_tag="VEL74_22225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65315.1"
                     /translation="FTIYIFLLDEEGLHIYDRHILRRAFSVTSPTYAFGLVHADHVWN
                     LDARGRSSVKRVGTTPR"
     gene            183..1322
                     /locus_tag="VEL74_22230"
     CDS             183..1322
                     /locus_tag="VEL74_22230"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HYG65316.1"
                     /translation="MIDTPSLPTLLEESRSVETSEAAPRLNPFLHVGEDRVYDPLTDR
                     TLTSGEPGYGLVRGLLAGSTTVEAAAESERALLSSQGLLLPADEDPSRRFLLKYVSLE
                     AHTVCNQSCYFCPVSIAPREDYFMPTELYERIVGELSAWRDTIEAVFMINYNEPTADK
                     RFVDQVRTLRAAGLPPAVLTNGTGLTPARIDALVELGGLRFLSINLSTLDRERYTKDR
                     GGDHLGLVLKHLDYAKDKALAEQMDMVVLGTGDENHKRDFAEISQRFAGSRFTVKYFE
                     VMDRAGYLQIGLKPAVPNQRLCGCENVGSRPLQHLHITPRGQCVLCCEDYNEAYVVGD
                     LTRESVEEVLTGPTMSLMRRWAYGLEEAPRDFICRNCTFALTRPA"
     gene            1343..2410
                     /locus_tag="VEL74_22235"
     CDS             1343..2410
                     /locus_tag="VEL74_22235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYG65317.1"
                     /translation="MRLAVILVHYHTPELAAEALAALRADVARVEGGIEWLLVDNGST
                     AEERALLERLPVRRIDPGRNLGYAGGVNLGVAESSAESILLMNPDVLVLPGCVPALLA
                     CLDGGAAVAGPRFYWDRTHRLLLPPAERRTRRDELLALLAAHCEPWAARARRRWRRHA
                     RRHWEAVRPLRSPWLSGSLLAVRRRAWERAGPFDEGFRLFYEETDWLLRVWRLGLPIW
                     YVPGAEAIHLYNQSAGQEPRAGQWFEESAQRFRRLHYGAWFARLLTAVDSRLGRAGLR
                     PPPAAPSMEIELPPGAAWVELSPNATGFPAAAERVSRSAGSRWAPPAEIAGALPPAGL
                     RVQVADAAGREAARWSLSSGA"
     gene            2412..3437
                     /locus_tag="VEL74_22240"
     CDS             2412..3437
                     /locus_tag="VEL74_22240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG65318.1"
                     /translation="MRITYVLPYPELGGGVKVALQHAALLRRQGFEVNVLGEGPAPDW
                     AGLEVPYHDYSGGLPSLPEQDLVIATFYTTIAVARDLRLGPIAHFCQGYEGGLVHLAP
                     AVPEIEAAYALDLPTLVVTPALGELLRTRFGRESAVVPPPLDPLFRPLPVWSRSWRPR
                     RAPQVAVPGIFEAEVKGVPTALAALASLKARGRGCRVLRFSLLALSAGERRLLEPDRY
                     LRAAPPAEIAAALRECDLLLLPSRAEEGFGLPLLEAMASGVPAVASRIPSTEYIGGAA
                     ALVAPGDAEGFATAAAELLDDARAWRRARRRGLAAALRFRPEEIAPRLAEAVRWAAGR
                     KAPALSS"
     gene            3448..4176
                     /locus_tag="VEL74_22245"
     CDS             3448..4176
                     /locus_tag="VEL74_22245"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HYG65319.1"
                     /translation="MHYDLSVVIPAYNEAERLGPGLRRAVDYLKRRGLSYEVLVVDDG
                     SRDATVQVAEGFAGEGVRVLRHERNRGKGGAVQTGLLASLGERVLLSDADFSTPIEEL
                     EKLEPRLRDAALVVGSRALASSDIRQHQPRHRELMGKTFNLIIRALGVRGLRDTQCGF
                     KLVRGDVGRALGAELTVEGFAYDVELVWLARRHGHHVVEVGVVWSDSPDSRVDPLRSS
                     LAMLRDVVRIRLRHRNLERSRGRR"
     gene            4173..6401
                     /locus_tag="VEL74_22250"
     CDS             4173..6401
                     /locus_tag="VEL74_22250"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HYG65320.1"
                     /translation="MSLNPALPVLALLGGAGLLWGLTGGAARPPRRGLGIAWGVLAVA
                     GIALLWPALSLPTGIPSPAADLAGLPPWQAEEGAAPSGNPLLRDVTFQIQPWLLFLRQ
                     ELRAGRLPFWNPWQFSGSPFWANGQSAPLFPLHLLFVVLPLQLGFVLLPWLRLVIGGC
                     GVWALGRELGLSPPAALLAAITFPLSGMLVSFLLFPMGNALALVPWVLWAVERIAADT
                     GSGGSGGTWKPLAIAAGFQLLAGHPETAAHTALLAALYLLVRGSAAPLRAWLRFVAGW
                     GIAGAIAAVQLLPLALTLLETSRWHHLTEAVHPPFRLLLQQPLRLVLPQLYGHPALGT
                     WWGPFNYSATAVYAGALALPLAAAGLVRCRGDRRWLALAVLLAFSFAAAYHWPGVREL
                     LGALPVLGRALHHRLIFGVELGLALLVGAGCDRWLEGKGRGLLAGAVATGLLLATAWL
                     LYAGEWVSRGLTAGQVAWTVGVAGASLLLVLSLRLSRERRWAVWPLLPALALCDLWLA
                     HGAINPGLPRERLYPETGAVRFLRDEPGRPGRVAGLGQALRPNAAMVYGLRDVRGDDP
                     VKLERYEAVYRTFAPSDPVYYQPVERWGELWLDRLGVRWVLAGPSEPPPAADWGVSYV
                     DKDGRVYERPGALPLVRWSGGAGEVEVARQDPGLWEIDWRAPRSGLLVVAETWDRGWS
                     AEVDGRPVAVLRVDGALLGARLGPGSGRLTLRYRPPGIAAGAALSLGGLALLLLRRRR
                     SR"
     gene            6398..7318
                     /locus_tag="VEL74_22255"
     CDS             6398..7318
                     /locus_tag="VEL74_22255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65321.1"
                     /translation="MIEMRPTTDNDLPALSALFADRFGHPLGAEEWLWKYRQIPGEGR
                     SLVAVEEGEVVAHAGALRLPARWRGGDAGIWQLVDFAGTTRRGGLRPALVELGRRLLA
                     DLPGRVDAPWIFGFPSERHFRLGQRVFGYRPLTVLTPLGGEIPEGVPGPLETGDSCGE
                     WAGAVWERCGLLGVRRSAGFLNWRYHARPHRYYRFYRLYEGDLEGLAVFAFVGEEAWA
                     AEVWLPAPGRWYPAMSAVAADLRASGLRTWRFWPSPGGRLASLYQALGLRELEERRFI
                     GCRGRADGGGEAPEAAAAGFHYAMGDYDLV"
     gene            7305..9614
                     /locus_tag="VEL74_22260"
     CDS             7305..9614
                     /locus_tag="VEL74_22260"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYG65322.1"
                     /translation="MTWSDVARGIALAGLLLLGGCSREPAGFLLLPLVQAKSYSLDAS
                     DSSGDGLSFPKAPEVRAVEVGHERRPAVLTTPGRWRWRGRVPEGARLHVGLQALPQAW
                     AAARALEAEVVVRDGGRREILEVARATAKEDRAWIDFDADLRRYAGRDVTLELSASLV
                     GLPADRRSANLIAWGPVNIANGPEEDRTNREEERRPNILFILVDTLRHDHLTPYGYRR
                     DTSPEIARRLAARGTVVEDAYSQAPWTLPSVVSFMTGRYPGELLGADLATYGVPEGVP
                     TLAEMLSALGYETGGFIANPTLHAGAGFGRGFRTFHAPPAEVSWMLDKHADDLNARVL
                     PWLSAYQNQERPFFLYVHYIDPHDPYMNPDMVDGYKSPFMPEYKGPIAGDWVHGIYGG
                     KLQLGDPEADVAHIRALYDNEIRYVDRHIGQLLAAIDPKVLANTLIVLTSDHGEELYD
                     HGGWKHGQTLYEEQIHVPLIVRWDGHVPAGKRLGGAVSLLDLVPTLMMAAAGGKDVRP
                     DPAWQGIDLLPALTGGKPLPRRSVFSQHLSGGPLRAAAIMDRQKLLLFNSREPFDAED
                     SLQSYLWQKDLQRLRRVELYDLSRDPGEKENLADARPDGVAGLAPAIHSRLGRELPGL
                     WMVASASGSGRGSGTAGSITFEKPPARWVPYFLGPDDRVELNGNRLRFELSPDRIEKG
                     FRVEGDFGAVLAVEAPAGALRVGDSGRIYPGGVVRRLSREALRVQGWPAAERRGALWM
                     GMPDIGAVKRRAEADPETEKRLRALGYIE"
     gene            9611..11578
                     /locus_tag="VEL74_22265"
     CDS             9611..11578
                     /locus_tag="VEL74_22265"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HYG65323.1"
                     /translation="MSHPLSPPGWTERVQAASALVIAPHYDDEVLGCGGLVKQLTAAD
                     AAVRVLFLTDGGGGTEEIADREAYRRLRREEAGRAAGVLGLAGCDHLGLPDGSLELYL
                     DEAEQGIRRALLTQRPELVLVPSPLEVTPDHRAAFAAFHRLIAPLRDQDPEGGPAAEE
                     LRRLRILLYEVNHPGYPDLLVDVSAQAGTLAAAMSCYGSQEERHPYHSAGLGLRRFRT
                     LSLPRASGVELAEGYRLLLPEDLTTRSPAQLLRFLGGAPERLEVREGPKISVVVRTRD
                     RPELLAEALGSLAEGHYRRAEVVLVNDGGTPPRVPDGFPLPVVRVDLAAEGNTEGNRG
                     SGRAAAAQAGVAAATGDFVAFLDDDDLAAPEHLATLADMVEAAGVRVAYTDAAVAIYE
                     LASGEAAEGSGWVCRERRLPYSRDFDPDVLLVDNYIPFNTLLIERDLFARVGPFDPTL
                     PFFEDWDFLIRLSAVTPFHHLARVTCEYRHFRGGVHHIFGERPRERSDFLEVKARVLA
                     KHAGLLTPEAMARAVDTLRAELVNEREARSATRRELAARTGELGTLRHAHEELARDRA
                     RLEERFHALNGELESVRAELSHWQEDHGRLGAEARRLYEEEGKLRQAVDEQAAHLGRT
                     YAEIERLNGLIRDMQSTRAWRAHQWWQRRSS"
     gene            11554..12840
                     /locus_tag="VEL74_22270"
     CDS             11554..12840
                     /locus_tag="VEL74_22270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HYG65324.1"
                     /translation="MVAAPQLMTRIALLSSEPVRPLMAGIGIRYLELARRLPAYGIDV
                     VLVSPAAPEDTRELSVGTADVRQFERGRLAAILGDCDGAVAQGQLANDLLLEMPALPV
                     AIDLYDPWLVENFAYLDTLGLGPFRNDHATWVLQMSRGDFFLCSSEEQRTFYLGFLAA
                     LGRVHPERVVRDPDLADLVQPVPFGVPGELPPHRPVLPSRPAGETRLLFGGLYDWYDP
                     WTLLDALESLDRPGWALYLIRNPNPESTPQRLFREVEARCRRLGWWGSRVRVLDWTPA
                     DRRYDLLRDVDLLVAPHRPSLETRLSLRTRFLDALAADCPVVTSDGGAMSRLLREHRA
                     GWVVPPGDAAALARALVEAVEDSAAREERRRGARELLADFQWDRALAPLVRFCRNPWR
                     DESKERFAHRPGVEVPADSLAWKVRRRLKRLVGAGA"
     gene            12837..14180
                     /locus_tag="VEL74_22275"
     CDS             12837..14180
                     /locus_tag="VEL74_22275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYG65325.1"
                     /translation="MSAPSRVSVAVLSWNGRHHLETCLAALEEQADPGVPWEVLVLDN
                     GSTDGTVPWLEKHYGRHPRVRWIESPVNLGFCAGNNRLAAEARGDAIALLNNDTRPEP
                     GWLAALVDALAAAPDDVAAVSGKIVDWEGEHLDFGRGVMTFDGHAFQLDFRRPLSKAR
                     VPRGGEELLFACGGNMLVRRESFLAAGGFDESYFAYLEDVDLGWRLWSGGERVVFAPD
                     AVVRHRSSATSDLLGNYNRGFLFERNAYLTVYKNYEAGLWERLMPAVMLTLLTRTQTL
                     LLENNPGTGTLRLDPYSGWIANTAPAAAPPAVPVTLPPPRPLTPAGLAARWKEYGTRD
                     LAARGWRKLKQRVLPPPSPAAPEPPRLTDERTIAQFRVLTNLIGHLDRAARSRESIQR
                     RRRRSDREIFDRFPLYLVPTYPGDDPLFANPAFTTWLPDDLPIVRAKLEEVMEAG"
     gene            14261..14632
                     /locus_tag="VEL74_22280"
     CDS             14261..14632
                     /locus_tag="VEL74_22280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65326.1"
                     /translation="MSEIEQLEQQVENLSAEDFARFRAWFIEFDSRHWDQQIKQHLKA
                     GRLDNLIAEALEDLKAGSARAALEGEEDSEGRLARLERQGLLRRASAPLSGDDLMTPL
                     PQPMEGASVLNALLAERREGR"
     gene            complement(14686..>14924)
                     /locus_tag="VEL74_22285"
     CDS             complement(14686..>14924)
                     /locus_tag="VEL74_22285"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HYG65327.1"
                     /translation="PGTLEHFLTERYCLYARKRDGSILRSEVHHGPWPLQGAAADIEV
                     NEVAGPQGIEIQGPPALLHFSRRIDVAVWSVQRV"
BASE COUNT         1842 a         5512 c         5361 g         2209 t
ORIGIN      
        1 ttcacgatat atatttttct cctggacgaa gagggtctcc atatctacga tcgacacatc
       61 ctgcggcggg ccttcagcgt caccagcccc acctacgcgt tcgggctcgt gcacgcggac
      121 cacgtctgga atctggacgc gcggggccgc tcatccgtaa aaagagtagg gacaacaccg
      181 agatgatcga tacacccagc ctgcccaccc tgctcgaaga gagccgctcc gtggagacca
      241 gcgaggccgc tccgcggttg aacccgttcc tccacgtcgg cgaggaccgc gtctacgatc
      301 cgctgaccga ccgcacgctg acctccggcg agccgggcta cggcctggtg cgggggctgc
      361 tcgccggctc caccaccgtc gaggcggccg ccgagagcga gcgggccctc ctctccagcc
      421 aggggctgct gctgccggcg gacgaggatc cgtcccgccg gttcctcctc aagtacgtgt
      481 cgctcgaggc gcacacggtc tgcaaccagt cctgctactt ctgtccggtc tcgatcgcgc
      541 cgcgcgagga ctacttcatg cccaccgagc tgtacgagcg gatcgtgggt gagctgtccg
      601 cctggcgcga caccatcgag gcggtcttca tgatcaacta caacgagccg acggccgaca
      661 agcggttcgt cgaccaggtg cgcaccctcc gcgcggcggg gctgccgccg gcggtgctca
      721 ccaacggcac cggcctgacg ccggcccgga tcgacgccct ggtggagctc ggcggtttgc
      781 gcttcctctc gatcaacctg tcgaccctcg accgcgagcg ctacaccaag gaccggggcg
      841 gcgatcacct cggcctagtc ctgaagcatc tcgactacgc caaggacaag gcgctcgccg
      901 agcagatgga catggtggtc ctggggaccg gcgacgagaa ccacaagcgg gacttcgccg
      961 agatctcgca acgcttcgcc ggcagccgct tcacggtcaa gtacttcgag gtcatggacc
     1021 gcgccgggta cctgcagatc ggcctcaagc cggcggtgcc caaccagcgc ctctgcggct
     1081 gcgagaacgt cggctcccgg ccgctccagc acctccacat cacgccgcgc gggcagtgcg
     1141 tcctctgttg cgaggactat aacgaggcct acgtggtggg cgacctcacc cgcgagagcg
     1201 tcgaggaggt gctcaccggg ccgacgatgt cgctgatgcg gcggtgggcc tacggcctcg
     1261 aagaggcgcc ccgcgatttc atctgccgga actgcacctt cgcgctgacg cggcccgcgt
     1321 gatcctcccg cccggaggac ccatgcgcct cgcggtgatc ctggtccact accacacgcc
     1381 cgagctggcg gcggaggcgc tggccgccct gcgcgccgat gtcgccaggg tggagggagg
     1441 gatcgagtgg ctgctggtgg acaacggcag caccgccgag gagcgcgcgt tgctcgagcg
     1501 gctgccggtg cggcggatcg atcccggacg caacctcggc tacgcgggcg gcgtcaacct
     1561 cggcgtggcg gagtcgagcg ccgagtcgat cctgctcatg aaccccgacg tcctcgtgct
     1621 gccggggtgt gtccccgccc tcctcgcctg cctcgacggc ggcgccgccg tcgccggccc
     1681 gcgcttttat tgggaccgca cccaccgcct gctcctgccg ccggccgagc gccggacccg
     1741 ccgcgacgag ctgctcgccc tcctcgccgc acactgcgag ccgtgggccg cccgtgcccg
     1801 ccgccgctgg cgccggcacg cccgccggca ctgggaggcg gtccgtcccc tgcgcagccc
     1861 atggctgagc ggcagcctgc tcgccgtgcg gcgccgcgcg tgggagaggg ccggtccgtt
     1921 cgacgaaggg ttccggctgt tctatgagga gaccgactgg ctgctgcgcg tctggcgcct
     1981 gggcctcccc atctggtacg tgccgggcgc cgaggcgatc cacctctaca accagagcgc
     2041 cggccaggag ccgcgggccg ggcagtggtt cgaggagtcg gcccagcgct tccggcgcct
     2101 gcactacggc gcctggttcg ccaggctcct gacggcggtc gattcgcggc tcggccgggc
     2161 cgggctccgt ccacctcccg cggccccctc catggagatc gagctgccgc ccggcgccgc
     2221 ctgggtggag ctatccccca acgccaccgg cttccccgcc gccgcggagc gggtctcccg
     2281 gtccgcgggc tcccgctggg cgccgccggc ggagatcgcg ggggctctgc cgccggccgg
     2341 gctccgggtg caggtcgccg acgccgccgg ccgggaggcc gcccgctggt cgctgtcttc
     2401 cggagcctga ggtgcggatc acctacgtcc tcccctatcc cgagctgggc ggcggcgtca
     2461 aagtggcgct gcagcacgcg gcgctgctgc ggcgccaggg cttcgaggtg aacgtgctgg
     2521 gggaggggcc ggcgccggac tgggcggggc tcgaggttcc gtaccacgac tacagcggcg
     2581 gactgccctc cctgccggag caggatctgg tgatcgccac cttctacacg acgatcgccg
     2641 tggcccgcga cctccggctc gggcccatcg cccacttctg tcaggggtat gaggggggac
     2701 tcgtccacct cgcgccggcc gtccccgaga tcgaggcggc ctacgcgctc gatctcccca
     2761 ccttggtagt cacgccggct ctgggggagc tccttcgcac gcgattcggc cgggagagcg
     2821 ccgtggtccc gccgcccctc gaccctctgt tccggcccct gccggtctgg agccgctcct
     2881 ggagaccgcg ccgggctccc caggtggcgg tgccggggat cttcgaggcc gaggtgaagg
     2941 gggtaccgac cgccctcgcc gctctggcga gcctcaaggc gcgcgggcgc ggctgccggg
     3001 tgctccgctt ctcgctcctg gcgctgtcag ccggggagcg gcgcctcctg gagcccgacc
     3061 gctacctccg cgccgcgccg cccgccgaga tcgccgccgc cctccgcgag tgcgacctcc
     3121 tgctgctgcc gtcgcgggcc gaggagggct tcgggctgcc gctcctggag gccatggcgt
     3181 cgggggtgcc ggcggtggcc tcccgcatcc cctcgaccga gtacatcggc ggcgcggcgg
     3241 ccctggtcgc ccccggcgac gccgaggggt tcgccaccgc ggcggcggag ctgctggacg
     3301 atgcccgggc ttggcggcgg gcgcggcgcc gagggctcgc ggcggccctg cggttccggc
     3361 ccgaagagat cgccccccgc ctcgccgagg ccgtgcgctg ggcagccgga cggaaggcgc
     3421 ccgctctctc gtcatagaat ccgcgccatg cactacgacc tctccgtggt catccccgcc
     3481 tacaacgagg cggagcggct gggacccggc cttcgccggg cggtcgatta cctgaagcgc
     3541 cgcgggctgt cctacgaggt actggtggtc gacgacggca gccgcgacgc caccgtccag
     3601 gtggcggagg ggttcgcggg ggagggggtg cgggtgctgc ggcacgaacg caaccggggg
     3661 aagggggggg ccgtccagac cggcctcttg gccagcctgg gggagcgggt gctgctgtcg
     3721 gatgccgatt tctctacccc catcgaggag ctggagaagc tggagccccg gctgcgcgac
     3781 gccgccctgg tcgtcggcag ccgcgccctc gccagctcgg acatccggca gcaccagccg
     3841 cggcatcggg agctcatggg caagacgttc aacctgatca tccgcgccct gggcgtccgc
     3901 ggcctgcgcg acacccagtg cggcttcaag ctggtccgcg gggacgtggg ccgcgccctc
     3961 ggcgccgagc tgacggtgga ggggttcgcc tacgatgtcg agctggtctg gctggcgcgc
     4021 cgccacggcc accatgtcgt cgaggtgggg gtggtctggt ccgactcgcc cgactcgcgg
     4081 gtggaccccc tgcgctcctc gctcgccatg ctgcgggacg tggtccggat ccggctgcgt
     4141 caccggaacc tggaacggag ccggggccgg cggtgagcct gaacccggcc ctgccggtgc
     4201 tggcgctgct gggaggggct gggctcctct ggggcctgac cggcggggcg gcgaggccgc
     4261 cgcggcgggg gctcggcatc gcctgggggg tgctggctgt cgcggggatc gccctgttgt
     4321 ggccagcgct ctccctgccc accggcatcc cgagcccggc cgcggatctc gccgggctcc
     4381 ctccctggca ggcggaggag ggggccgctc cttccgggaa ccccctgctg cgggacgtga
     4441 cgttccagat ccagccctgg ctcctcttcc tccggcagga gctgcgggcg ggacggctgc
     4501 cgttctggaa cccctggcag ttctccggct cgccgttctg ggccaacggc cagtcggccc
     4561 ctctcttccc cctgcacctg ctgttcgtgg tcctccctct ccagctcggc ttcgtcctcc
     4621 tcccctggct gcggctggtg attggcggct gcggcgtctg ggccctcggc cgcgagttgg
     4681 ggctctcgcc gccggcggcg ctgctggccg ccatcacctt ccctctctcg gggatgctgg
     4741 tgagcttcct tctctttccc atgggcaacg ccctggcgct ggtgccctgg gtgctctggg
     4801 cggtggagcg gatcgcggcc gatactggga gcggggggag cgggggaacc tggaagccgc
     4861 tggcgatcgc ggccgggttc cagctcctgg ccgggcaccc ggagacggcg gcgcacacgg
     4921 cgctcctcgc ggcgctctat ctgctggtgc gtggatcggc ggcccccctc cgggcctggc
     4981 tccggttcgt cgccggctgg gggatcgcgg gagccatcgc cgcggtccaa ctcctccctc
     5041 tcgcgctgac gctgctggag acgagccgct ggcaccatct caccgaagcc gtccatcccc
     5101 ccttccggct gctgctgcaa cagcccctcc gcctggtcct cccccagctc tacggccatc
     5161 ccgcactcgg cacctggtgg gggcctttca actactcggc gaccgcggtc tatgccggag
     5221 ccctggcgct gcccctcgcc gccgccggcc tggtgcgctg ccggggggac cggcgatggc
     5281 tggcgctggc ggtgctgctc gccttttcat tcgccgcggc ctatcactgg cctggggtgc
     5341 gcgagctgct gggggcgctg ccggtgctgg gacgcgccct ccaccaccgg ctgatcttcg
     5401 gcgtcgagct cgggctggcc ctgctcgtcg gcgccggctg cgaccgctgg ctggagggga
     5461 aggggcgcgg cctgctggcc ggcgcggtgg cgacggggct gctcctcgcc acggcctggc
     5521 tgctgtacgc cggagagtgg gtgtcccggg gcctgaccgc cgggcaggtg gcgtggactg
     5581 tcggcgtcgc cggggcctcg ctcctcctcg ttctctccct gcggctgagc cgcgagcggc
     5641 gctgggctgt ctggccgctg ctgcccgccc tggcgctgtg cgatctctgg ctcgcgcacg
     5701 gcgccatcaa ccccgggctg cctcgggagc ggctgtaccc cgagaccggc gcggtgcgct
     5761 tcctgcgtga cgagccgggg aggccaggac gggtcgccgg cctggggcag gcgctgcggc
     5821 ccaacgccgc catggtctat ggtctacggg acgtgcgcgg tgacgatccg gtgaagctcg
     5881 agagatacga ggcggtctac cggaccttcg cgccgtccga ccccgtctac taccagccgg
     5941 tggagcgctg gggggagctc tggctcgacc ggctgggggt ccgttgggtc ctggccggcc
     6001 cgtccgagcc gccgcccgca gcggattggg gggtctccta cgtagataag gacgggaggg
     6061 tctacgagcg ccccggcgcc ctgccgctcg tccgctggtc cgggggagcc ggcgaggtgg
     6121 aggtagcgcg gcaggacccc ggcctctggg agatcgactg gcgggcgccc cggtccggcc
     6181 tgctggtggt ggccgagacc tgggacaggg gctggagcgc cgaggtggac ggccggccgg
     6241 ttgccgtact gcgggtggac ggcgcgctcc ttggcgcgcg gctcgggccg ggctccggac
     6301 ggctgaccct ccgctatcgt cccccgggga tcgcggccgg cgcggccctg agcctgggcg
     6361 ggctggccct gctgctcctg cggcggagaa gaagtcgatg atcgagatga gaccgacgac
     6421 cgacaacgac ctgccggcgc tgagcgcgct gttcgccgac cgcttcgggc accccctggg
     6481 cgccgaggag tggctctgga agtaccggca gatccccggc gaggggcgga gcctggtggc
     6541 cgtcgaggaa ggggaggtgg tggcccatgc cggggcgctg cggctgccgg cccggtggcg
     6601 cgggggcgac gccggcatct ggcagctcgt cgacttcgcc ggcacgaccc ggcggggcgg
     6661 gctgcgaccg gccctggtcg agctcggccg ccggctgctc gcggaccttc ccggccgggt
     6721 ggacgcaccc tggatcttcg gcttccccag cgagcgccac ttcaggctgg ggcagcgggt
     6781 cttcggctat cggccgctga cggtcctgac ccctctgggt ggggagatcc ccgagggcgt
     6841 tcccggtccg ctcgagaccg gcgactcgtg cggggagtgg gccggggcgg tctgggagcg
     6901 ctgcggtctc ctgggggtcc gccgctcggc cggcttcctc aactggcgct accatgcccg
     6961 gccccaccgc tactatcgct tctatcgcct gtacgagggg gacctcgaag gactggccgt
     7021 gttcgcgttc gtaggggagg aggcctgggc ggccgaggtc tggcttcccg ctccgggccg
     7081 gtggtaccct gcgatgtccg ccgtggccgc cgacctccgc gcgtccgggc tccgcacctg
     7141 gcgcttctgg ccttcgcccg gcggccgcct cgcctctctc taccaggcgc tcggcctgcg
     7201 agagctggag gagcggcggt tcatcggctg ccgcggccgg gcggatgggg gaggggaggc
     7261 gccggaggcc gccgcggccg gattccacta tgccatgggg gactatgacc tggtctgacg
     7321 tcgcgcgggg gatcgccctc gccgggctcc tgctgctggg cgggtgtagc cgcgagcccg
     7381 cggggttcct gctgctgccg ctggtgcagg cgaagagcta ttcgctcgat gccagcgaca
     7441 gcagcggaga cgggctctcc ttcccgaagg cgcccgaggt gcgcgccgtc gaggtcggtc
     7501 acgagcggcg gccggcggtg ctcacgaccc ctggccgttg gcgttggcgt ggccgggtgc
     7561 cggagggggc gcggctgcac gtcggtctcc aggcgctgcc gcaggcctgg gccgcggcgc
     7621 gggctctcga ggccgaggtg gtcgtacgcg acggtggccg ccgcgagatc ctggaggtgg
     7681 cccgcgccac cgccaaggag gaccgggcct ggatcgactt cgacgccgac ctgagacgct
     7741 acgccggccg cgacgtcacc ctggagctct ccgcctccct cgtcggcctc cccgcggacc
     7801 ggcggagcgc caacctgatc gcctggggac cggtcaacat cgccaacggc ccggaggagg
     7861 accggacgaa ccgggaggag gagcggcgcc cgaacatcct cttcatcctg gtggacaccc
     7921 tccgccacga tcacctgacc ccctacggct accggcgcga cacctcgccc gagatcgcgc
     7981 gccgcctcgc cgcccgcggc accgtggtgg aggacgccta cagccaggcg ccgtggacgc
     8041 tgccgtccgt ggtctcgttc atgaccggcc gctaccccgg cgagctgctg ggggcggacc
     8101 tcgccaccta cggcgttccc gagggggtgc ccaccctggc ggagatgctg tcggcgctgg
     8161 gctacgagac cggcggcttc atcgccaacc cgaccctcca cgccggcgcc ggcttcggcc
     8221 gcggcttccg caccttccac gcgccgccgg ccgaggtgtc gtggatgctc gacaagcacg
     8281 ccgacgacct gaacgcccgc gtcctcccct ggctctccgc gtaccagaac caggagcggc
     8341 cgttcttcct gtatgtccac tacatcgacc cgcacgaccc ctacatgaac ccggacatgg
     8401 tggatggcta caagtcgccg ttcatgcccg agtacaaggg gcccatcgcc ggcgactggg
     8461 tgcacggcat ctacggcggc aagctccagc tcggcgatcc cgaggccgac gtggcccaca
     8521 tccgggccct ctatgacaac gagatccgct acgtcgaccg tcacatcggc cagctcctgg
     8581 cggcgatcga ccccaaggtg cttgccaaca cgctgatcgt cctcacctcg gatcacggcg
     8641 aggagctgta cgaccacggc ggctggaagc atgggcagac gctctacgag gagcagatcc
     8701 acgtgccgct gatcgtccgt tgggacggcc acgtccccgc cggcaagagg ctcggcggcg
     8761 cggtctcgct cctcgacctc gtgccgaccc tgatgatggc cgcggcgggc ggcaaggacg
     8821 tccggccgga cccggcgtgg caggggatcg acctgctgcc cgccctcacc ggcggcaagc
     8881 cgctgcctcg gcgctcggtc ttctcccagc acctgagcgg cgggccgctg cgcgccgcgg
     8941 cgatcatgga ccgccagaag ctcctcctgt tcaactcgcg cgagccgttc gacgccgaag
     9001 acagcctcca gagctacctc tggcagaagg acctccagcg gctgcggcgg gtggagctgt
     9061 acgacctctc ccgcgacccc ggcgagaagg agaacctcgc cgacgcccgg ccggatggag
     9121 tggcggggct ggccccggcc atccacagcc ggctcggccg cgagctgccc ggcctctgga
     9181 tggtcgcgag cgcttccggc tcgggccgag gatcgggaac agccggcagc atcaccttcg
     9241 agaagccgcc cgcgcgctgg gtgccctact tcctggggcc ggacgaccgc gtcgagctca
     9301 acggcaaccg cctccgcttc gagctctctc ccgaccgcat cgagaagggg ttccgggtgg
     9361 agggggactt cggcgcggtg ctcgctgtgg aagcgccggc cggcgcgttg cgggtcggcg
     9421 actccggacg gatctatccc ggtggcgttg tcaggcgatt gagccgcgag gccctgcggg
     9481 tccaggggtg gccggcggcg gagcgccgcg gcgccctctg gatgggcatg cccgacatcg
     9541 gagcggtcaa gcggcgcgcc gaggccgacc cggagaccga gaaacgcctc cgcgccctgg
     9601 ggtacatcga atgagccatc ccctgtctcc ccccggctgg accgagcgcg tgcaggccgc
     9661 gagcgcgctg gtgatcgccc cgcactacga cgacgaggtg ctgggctgcg gcgggctcgt
     9721 gaagcagctc accgccgccg acgccgccgt gcgcgtcctc ttcctcaccg acggcggcgg
     9781 aggtaccgag gagatcgccg accgcgaggc ctatcgccgg ctgcgccgtg aggaggcggg
     9841 gcgggcggcc ggggtgctcg ggctcgccgg ctgcgatcac ctggggctgc cggacggctc
     9901 cctcgagctc tacctggacg aggcggagca ggggatccgc cgggctctcc tcacccagcg
     9961 cccggagctg gtgctggtcc cctcgccgct ggaggtgacc ccggaccacc gggccgcctt
    10021 cgccgccttc caccggctca tcgcgccgtt gcgcgaccag gatccggaag gggggccggc
    10081 ggccgaggag ctgcggcggc tgcgcatcct cctctatgag gtgaaccacc ccggctatcc
    10141 cgacctgctg gtggacgtct ccgcccaggc ggggaccctc gccgccgcca tgtcctgcta
    10201 cggcagccag gaggagcggc acccctacca cagcgccggc ctggggctgc gccggttccg
    10261 caccctgagc ctgccgcggg cctccggcgt cgagctggcc gagggctacc ggctgctgct
    10321 gccagaggac ctcactaccc gcagcccggc gcagctcctg cgcttcctgg gcggcgctcc
    10381 cgagcgcctc gaggtgcggg aagggccgaa gatctccgtc gtcgtccgca cccgcgaccg
    10441 gccggagctg ctcgccgagg cgctggggag cctggccgag gggcactacc ggcgcgccga
    10501 ggtggtgctg gtcaacgacg gcggcacgcc gccccgggtg cccgacggct tcccgctgcc
    10561 cgtggtgcgg gtggacctgg ccgccgaggg caataccgag gggaacaggg ggagcggccg
    10621 cgccgccgcg gcgcaggccg gagtcgcggc ggccaccggc gacttcgtgg ccttcctgga
    10681 cgacgacgac ctcgccgccc ccgagcacct ggcgacgctc gccgacatgg tcgaggccgc
    10741 cggcgtgcgg gtggcctaca ccgacgccgc cgtcgccatc tacgagctgg cgtccggcga
    10801 ggccgcggaa ggcagcggct gggtctgccg cgagcggcgc ctcccctaca gccgcgactt
    10861 cgatcccgac gtcctgctgg tggacaacta catcccgttc aacacgctgc tcatcgagcg
    10921 cgacctcttc gcccgggtgg ggccgttcga ccccacgctg ccgttcttcg aggattggga
    10981 cttcctgatc cgcctgtccg ccgtcacccc gttccaccat ctggcgcgcg tcacctgcga
    11041 gtaccggcac ttccggggcg gcgtccatca catcttcggc gagcggccgc gggagcgctc
    11101 cgacttcctg gaggtcaagg ccagggtgct cgccaagcac gccggcctcc tgaccccgga
    11161 ggcgatggcg cgcgccgtgg acaccctgcg cgccgagctg gtgaacgagc gcgaggcccg
    11221 gtccgccacc cggcgcgagt tggcggcgcg gacgggggag ctcggcaccc tgcgccacgc
    11281 ccacgaggag ctggcccgcg accgggcgcg gctggaggag cgcttccacg cgctcaacgg
    11341 cgagctggag tcggtccggg ccgagctgtc ccactggcag gaggaccacg gccgcctcgg
    11401 cgccgaggcc cggcgcctct acgaggagga ggggaagctc cgccaggccg tggacgagca
    11461 ggccgcccac ctgggccgga cgtacgcgga gatcgagcgg ctcaacggcc tgatccgcga
    11521 catgcagtcg acccgcgcct ggcgcgccca ccagtggtgg cagcgccgca gctcatgacc
    11581 cgcatcgccc tcctctccag cgagccggtc cgccccctca tggccgggat cggcatccgc
    11641 tacctggagc tggcgcgccg cctcccggcc tacggcatcg acgtcgtcct ggtctccccg
    11701 gcggcgccgg aggatacccg cgagctgtcg gtggggaccg ccgacgtgcg gcaattcgag
    11761 cgcggccgtc tcgccgcgat cctcggcgac tgcgacggcg ccgtggccca ggggcagctc
    11821 gccaacgacc tgctgctgga gatgccggcg ctccccgtgg ccatcgacct ctacgatccc
    11881 tggctggtgg agaacttcgc ctacctcgac accctgggcc tgggcccctt ccgcaacgac
    11941 cacgccacct gggtgctgca gatgtcgcgc ggggatttct tcctctgctc gtccgaggag
    12001 cagcggacgt tctacctcgg cttcctcgcc gcgctggggc gggtccatcc cgagcgagtg
    12061 gtgcgggacc ccgacctggc ggacctcgtc cagccggtgc cgttcggggt gcccggcgag
    12121 ctgccgccgc accgtcccgt gctgccgtcg cgcccggccg gtgagacgcg cctcctcttc
    12181 ggcggcctct acgactggta cgacccctgg acgctgctcg acgccctgga atcgctggac
    12241 cgtcccggct gggcgctcta cctgatccgc aaccccaacc cggagagcac gccgcagcgc
    12301 ctgttccgcg aggtggaggc gcgctgccgg cgactcggct ggtgggggag ccgcgtccgc
    12361 gtcctcgact ggaccccggc cgaccggcgc tacgacctgc tgcgcgacgt cgacctcctg
    12421 gtggcgccgc accggccgag cctggagacc cggctctccc tgcgtacccg cttcctcgac
    12481 gccctcgccg ccgactgccc ggtggtgacc agcgacggcg gcgccatgag ccgtctcctc
    12541 cgcgagcatc gtgccggctg ggtggtgccg cccggcgacg cggcggcgct cgcccgggcg
    12601 ctggtggagg cagtcgagga ttcggcggcg cgggaggagc ggcgccgggg ggcgcgggag
    12661 ctgctggccg atttccagtg ggaccgggcg ctcgcgccgc tggtccgctt ctgccgcaat
    12721 ccctggcggg acgagtccaa ggagcgcttc gcccaccgtc ctggagtgga ggtgccggcg
    12781 gactccctcg cctggaaggt ccgccgccgt ctcaagcggc tggtgggggc gggagcgtga
    12841 gcgccccctc gcgcgtctcg gtggccgtcc tctcctggaa cggccgccac cacctggaga
    12901 cctgcctcgc cgccctggag gagcaggccg atcccggcgt cccctgggag gtgctggtcc
    12961 tcgacaacgg ctcgacggac ggcacggtcc cctggctcga gaagcactac ggccggcatc
    13021 cgcgggtgcg gtggatcgag agcccggtga acctcggctt ctgcgccggc aacaaccgtc
    13081 tggcggcgga ggcccggggg gacgccatcg ccctgctcaa caacgacacc cgtccggagc
    13141 cgggctggct cgccgccctg gtcgacgcct tagccgccgc gccggacgac gtcgccgcgg
    13201 tctcgggcaa gatcgtcgac tgggaaggcg agcacctcga cttcggccgc ggcgtcatga
    13261 ctttcgacgg ccacgccttc caactcgact tccggcgccc actgtccaaa gcgagggtcc
    13321 cgaggggcgg ggaggagctc ctcttcgcct gcggcggcaa catgctggtg cgccgcgagt
    13381 ccttcctggc cgccggcggc ttcgacgaga gctacttcgc ctatctggag gacgtggacc
    13441 tgggctggcg cctctggtcc ggaggggagc gggtggtctt cgccccggac gccgtggtgc
    13501 gccaccggtc gagcgccacg agcgacctgc tgggcaacta caaccgcggc ttcctcttcg
    13561 agcgcaacgc ctacctgacg gtctacaaga actacgaggc tggcctctgg gagcggctga
    13621 tgcccgcggt gatgctgacg ctcctcaccc gcacgcagac gctgctcctc gagaacaacc
    13681 ccggcaccgg caccctgcgt ctcgacccct actcgggctg gatcgccaac accgccccgg
    13741 ccgcggcgcc tcccgccgtg cccgtgacgc tgccaccccc ccgtccgctg acgccggccg
    13801 gcctggccgc gcggtggaag gagtacggca cgcgggacct cgccgcccgc ggctggagga
    13861 agctcaagca gcgggtgctg ccgccgccgt cccctgccgc gccggagccg ccgcgcctga
    13921 ccgacgagcg caccatcgcc cagttccggg tgctgaccaa cctgatcggc cacctggacc
    13981 gcgccgcccg cagccgcgag tccatccagc gccgccgccg ccggagcgac cgcgagatct
    14041 tcgaccgctt ccccctctac ctcgtcccca cctaccctgg cgacgacccc ctcttcgcca
    14101 atcccgcctt caccacctgg ctgccggacg acttgccgat cgtgcgggcg aagctggaag
    14161 aggtgatgga ggcggggtga gcttcgccct cccagctctt ccggtaagcc tctctcccgc
    14221 tgctagaatg agggtgcaac tttcttctgt ggagaatgcc atgagcgaaa tcgagcaact
    14281 ggagcagcaa gtcgagaacc tttctgccga ggatttcgcg agatttcgcg cctggtttat
    14341 tgagttcgac tcccgccact gggaccagca gatcaaacaa cacctcaagg cgggcaggct
    14401 ggacaacctc atcgccgaag ctctcgagga tctcaaggct ggtagcgcgc gcgctgcgct
    14461 ggagggggag gaagattccg aggggcggct tgcccggctg gagcggcagg ggcttctgcg
    14521 ccgagcctcg gctccgcttt ccggagacga tctgatgact cccttgcctc agcccatgga
    14581 gggggcgagc gtccttaatg ctctcctcgc cgagcgaaga gaaggccgtt gaggcgctgc
    14641 gggactttgt gggtagcggg gatttggtgg ttgcacgggt gcaacctata cccgctgcac
    14701 tgaccacacc gccacgtcga tcctccgact gaagtggagc agggccggcg gtccctggat
    14761 ctcgattccc tgcggtcccg ccacctcgtt gacctcgatg tcggccgcgg cgccctggag
    14821 cggccagggg ccgtggtgca cctcgctgcg caggatcgat ccgtcccgct tccgggcata
    14881 gaggcaatag cgttcggtca ggaagtgctc cagcgtcccg ggct
//
LOCUS       DASPJI010000793         4718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_398606, whole genome shotgun sequence.
ACCESSION   DASPJI010000793 DASPJI010000000
VERSION     DASPJI010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398606"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4718
                     /locus_tag="VEL74_22290"
     CDS             <1..>4718
                     /locus_tag="VEL74_22290"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65328.1"
                     /translation="RALVACVALQPGSDADAASLRAALAARLPDFMVPAHLLFLDVLP
                     LNPNGKVDRRALASLPLAEANAPSARGRVTRGLVEELVAGLFAEVLGLDEAGADDDFF
                     ASGGHSLLATQLVSRAGRMFGVELPLRTLFDAPTVAGFAAAVEAALFSMGSGDAGAPP
                     IVPLPRRAEPPHLPVEDGLPLSFAQQRLWFLDRLVPDNPFYNIYGAVGLPGAVDLSAL
                     RRAFQEIGCRHESLRTTFAAVGGRPVQVIAPDLPLSIPLIDLGALPLEWARAEDARLS
                     GEESRRPFDLARGPLVRAVVVRLRNGAHTLQVNVHHIVADGWSMGILQRELGVLYGAF
                     ASGAAGTEPVLPALPIQYADFAVWQRGWLQGEVLEDQLAYWRRRLAGAPETLDLPLDR
                     PRPAHESFRGSAEPFVLSEALSQRLAGLTRRLGATFPMVLLAGFEALLSRYTGQDDLA
                     LGLAIANRNRREIEDLIGFFVNTLVLRGDLAAASGFSELVTQVRENALEAYAHQDLPF
                     EKLVEELQPERSLGRNPLVQVMCSFENFARGGDAGRAAALAELGREAVDTGTAKFDLT
                     LYVVGLGEPVRGFLQYNSDLFDATTMRRLLDHFAGLLTVAAREPDAPLATLPLLEAAG
                     RHQLVHEWNDTAADYPAEAGLHELFEEEASRAPDSVAVDSDTGSLTYGELARRARALA
                     RRLQEAGVGPESRVGVCLGRSPEMVVALLAILRSGGAYVPLDPGYPRERLDFMLRDAG
                     VRVLVARAPLPPALPDVSQARITLIDPNARVEMAGVAGISGGGDRLAYVMYTSGSTGT
                     PKAVGVPHRAVARLVRRQNGYARFGPDEVWLQLAPVSFDASTLEIWGALLHGGRLAVA
                     PAGVLSLEELGHTLSRHGVTSLWLTAGLFHQLVDHRLEALRPVRQLLAGGEALSVPHV
                     RRVLEALPGTRLVNGYGPTENTTFTCCHPLAGPADLGAPLQTVPLGRPIGHTRVHILD
                     RQDLAVPLGVAGELCTGGDGLARGYLGRPELTAAVFVPDPFGKPGERLYRTGDRARLL
                     PDGRVEFLGRLDQQVKLRGFRIEPGEVEARLSQHPAILQSAVVVREDEPGDRRLVAYV
                     VQDPGFRPESGDAGAEQVSQWSALFDDLYEQESAEADPAFNIIGWESSYTGRPLGREE
                     MREWVEDTVGRILSLEPRRVLELGCGTGLLLFRVAPRADLYVGTDISRRALDYVESRL
                     APTGLDSSRVRLLHRAADCFDGLEPGSFDAVVLNSVAQYFPDAEYLSRVLEQAVAAVR
                     PGGAVFVGDLRSLPLLDAFHTSVELFQAEDNLPLDHLRARVQARHGQEGELVVAPAFF
                     ADLARRLPGIQRVEVHPERGRIHNELTRFRYQAVLRVGEEAAPVSAAPAAPVEIRWLD
                     WRRDGLDLEGLRRLLRERRPEGLGLLGIPNARTATDSAAVRLLAREDGLATAGELRRA
                     AELEAGEGVDPQALWDLAGELPYEVELGWASPGGDGRFDAVLRRRGTTLPPAATLLPQ
                     PSPAPERNDLIGLANDPLRGRSARRIAPMLREFLAASLPEHMVPSAFVLLNALPISPN
                     GKVDRRRLPVPELARPDAGR"
BASE COUNT          554 a         1677 c         1733 g          754 t
ORIGIN      
        1 ccgtgccctg gtggcctgcg tagcgctgca gccggggagc gacgccgacg ccgcgtccct
       61 gcgcgcggcg cttgccgctc gtctgcccga tttcatggtg ccggcccacc tcctgttcct
      121 ggacgtcctg cccctgaacc ccaacggcaa ggtggaccgg cgggcgcttg catccctgcc
      181 gctggcagag gccaacgcac cgagcgcccg gggccgcgtt acccgcggct tggtggaaga
      241 gctggtcgcg ggtctcttcg ccgaggtctt ggggctcgat gaggcgggtg ccgatgacga
      301 cttctttgcc tccggcgggc attcgctcct ggcgacccag ctcgtctcac gggcgggacg
      361 gatgttcggt gtcgagctgc cgctgcggac gctgttcgac gctccgacgg tggcgggctt
      421 tgcggcggcg gtggaggcgg cgctgttttc catggggtcg ggagacgccg gggcgccgcc
      481 catcgttccc ctgccgcgcc gggccgagcc gccccacctg cccgtcgagg acggcctgcc
      541 gctctccttc gcccagcagc gcctctggtt cctggaccgg ctggtgccgg acaacccgtt
      601 ctacaacatc tatggggctg tagggttacc cggggctgtc gacctgtccg ccctgcgccg
      661 ggccttccag gagattggat gccggcacga gtccctgcgc accaccttcg cggcggtggg
      721 agggcggccg gtccaggtga tcgcgccgga tctgccgctc tcgatccctc tgatcgacct
      781 cggtgccctc cctctggagt gggcgcgagc cgaggacgcg cgactctcgg gcgaggagtc
      841 gcgccgaccc ttcgatctgg cgcgcgggcc cctggtccgt gccgtggtgg tgcgcctgag
      901 aaacggggcg cataccctgc aggtcaacgt ccaccacatc gtggccgatg gctggtccat
      961 gggcatcctg caacgtgagc tgggggtgct ctacggtgcg ttcgcctccg gggccgccgg
     1021 gaccgagcct gtgctcccgg cgctgcccat ccagtacgcc gacttcgcgg tctggcagcg
     1081 gggctggctc cagggcgagg tgctggagga ccagctcgcc tactggcggc gccggctggc
     1141 cggtgcgccc gagaccctgg atctccccct cgaccggccg cgtcccgccc acgagagttt
     1201 ccgcggcagc gccgagccct tcgtcctgtc cgaagcactg tctcagaggc tggctggtct
     1261 cacccggcgc ctcggggcga ccttccccat ggtgcttctc gccggcttcg aggccctcct
     1321 ctcccgctac accggtcagg acgacctggc gctgggcctc gccatcgcca accggaaccg
     1381 ccgggagatc gaggacctca tcgggttctt cgtcaacacc ctggtgctgc gcggcgatct
     1441 cgccgccgcc tccggcttca gcgagctggt gacccaggta cgggagaacg ccctcgaggc
     1501 ctacgcccac caggacctgc cgttcgagaa attggtggaa gaactgcaac cggagcggag
     1561 cctgggacga aacccgctgg ttcaggtcat gtgctcgttc gagaacttcg cccgtggcgg
     1621 cgacgctggg cgggccgcgg ccttggccga gctggggcga gaggcggtgg acaccgggac
     1681 cgcgaagttc gacctgaccc tctacgtcgt cggcctcggc gagccggtgc gggggttcct
     1741 ccagtacaac agcgacctgt tcgacgccac caccatgcgg cgcctcctgg accatttcgc
     1801 gggtctgctg acggtggcgg cgcgggagcc ggacgcgcct ctggccacgt tgccgctgct
     1861 ggaagccgcg ggccggcatc agctcgtcca cgagtggaac gacaccgcgg cggactaccc
     1921 ggccgaggcg gggctccacg agctgttcga ggaggaggcg tcgcgtgctc cggacagcgt
     1981 ggcggtggac tcggatacgg gctcgctcac ctatggtgag ctcgcccggc gcgcccgggc
     2041 gttggcccgg cggctccagg aggccggtgt cggacccgag agccgggtgg gggtctgcct
     2101 cgggcgctcg cccgagatgg tggtcgctct cctcgcgatc ctgcggtcgg gcggagccta
     2161 tgtgccgctg gacccgggct atccccggga gcggctggac ttcatgctgc gggatgcggg
     2221 ggtgcgcgtg ctggtggcgc gggcgccgct acccccggca ctgccggatg tgtcccaggc
     2281 ccggatcacc ctcatcgatc cgaacgccag ggtggagatg gcaggcgtcg cggggatctc
     2341 cgggggcggc gaccggctgg cctacgtcat gtacacctcg ggctctacgg gcacgcccaa
     2401 ggcggtcggc gtgccgcacc gggccgtcgc gcggctggtg cggcggcaga acggttatgc
     2461 ccggttcggg ccggacgagg tctggctgca gctcgcgccc gtctccttcg acgcttcgac
     2521 gttggagatc tggggcgcgc tgctgcatgg tgggcggctg gccgtggcgc ctgccggcgt
     2581 cctctcgctg gaggagctgg ggcatacgct ctcgcggcac ggcgtcacct ccctgtggct
     2641 caccgcgggc ctcttccatc agctcgtgga ccaccgcctg gaggcgttgc ggccggtgcg
     2701 ccagctcctg gccggcggcg aagccctctc cgtcccccac gtccggcggg tgctggaggc
     2761 gcttcccggt acacggctcg tcaacggcta cgggccgacg gagaacacca ccttcacctg
     2821 ttgccatccg ctggccggtc cggctgatct gggagccccg ctgcagacag tgcctctcgg
     2881 ccggcccatc ggacacaccc gagtccacat cctggaccgc caggatctgg cggtgcccct
     2941 gggggtggcg ggcgagctct gcacaggggg cgacggcttg gcgcggggct atctggggcg
     3001 gccggagctg acagcggcgg tgttcgtccc cgatccattc ggcaagcctg gcgagcgtct
     3061 gtaccggacc ggcgaccggg cgcggcttct gcccgacggg cgggtcgagt tcctgggccg
     3121 tctcgaccag caggtcaagc tccggggctt tcggatcgag ccgggcgagg tggaggcgcg
     3181 gctttctcag catcctgcga tcctccagag cgccgtcgtc gtgcgcgagg atgagccggg
     3241 cgaccggcgt ctcgtcgcct acgtggtcca ggaccccggc ttccgacccg agagcggcga
     3301 cgccggcgcg gagcaggtgt ctcagtggag cgccctcttc gatgacctgt acgagcagga
     3361 gtccgccgag gcggacccgg cgttcaacat cattggttgg gagagcagct acaccggccg
     3421 gccgctcggc cgcgaggaga tgcgcgagtg ggtggaggac acggtggggc ggatcctctc
     3481 cctggagccg cgccgcgtcc tggagctggg ctgcggcacc ggcctcctcc tcttccgggt
     3541 ggcgccccga gccgacctct atgtcgggac cgacatctcc cggcgggccc tggactacgt
     3601 ggaaagccgg cttgcgccga cgggcctgga cagctctcgg gtgcggctcc tgcaccgcgc
     3661 cgcggactgc ttcgacggcc tggagccggg ctccttcgat gccgtggtcc tcaactcggt
     3721 agcccagtat ttcccggacg ccgagtacct gtcgcgggtg ctggagcagg cggtggccgc
     3781 cgtacggccg ggaggcgcgg tcttcgtcgg cgacctgcgg agcctgccgc tgctggacgc
     3841 cttccatacc tctgtggagc tgttccaggc agaggacaac ctgcccctgg atcatcttcg
     3901 ggcgagggtg caggcgcggc acgggcagga gggcgagctg gtggtggccc cagccttctt
     3961 cgccgacctg gcgcggcggc tccccgggat tcagcgggtg gaggtccacc ccgagcgcgg
     4021 gcggatccat aacgagctca cccgcttccg ctatcaggcc gtgctgcggg tgggggagga
     4081 ggccgcgccg gtctcggccg ccccggcggc cccggtggag atccgctggc tcgactggcg
     4141 ccgcgacggc ctcgacctcg aaggcctgcg gcggctcctc cgcgagcggc gccccgaggg
     4201 cctgggcctc ctcgggattc ccaacgcccg caccgccact gactccgcgg cggtccgcct
     4261 gctggcacgg gaggacggcc tggcgaccgc cggcgagctg cgtcgtgccg ccgagctgga
     4321 agccggcgag ggagtggacc cccaggccct ctgggacctg gccggtgagc tcccctacga
     4381 ggtggagctg ggttgggcct caccgggcgg cgacgggcgc ttcgacgcgg tgctgcggcg
     4441 gcgcggcacc accctgccgc cagccgccac gctgctgccg cagccgtctc cggcgcccga
     4501 gcgcaacgac ctcatcggcc tcgcgaatga tcctctccgg gggcggtccg cgcggcgtat
     4561 cgcgcccatg ttgcgggagt ttctggccgc gagcctcccg gagcacatgg tgccgtccgc
     4621 cttcgtgctc ctcaatgctc tgcccatctc gcccaatggc aaggtggacc ggcggcggct
     4681 gccggtgccc gagctggcgc ggcccgacgc gggccggt
//
LOCUS       DASPJI010000794         3893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_398651, whole genome shotgun sequence.
ACCESSION   DASPJI010000794 DASPJI010000000
VERSION     DASPJI010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398651"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..677
                     /locus_tag="VEL74_22295"
     CDS             24..677
                     /locus_tag="VEL74_22295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYG65329.1"
                     /translation="MPIRLILADDHPIILDGLQQLFRLEPDFEVVACCRDGEETLKAL
                     RLHAPDILVLDIRMPRGDGLAVLRALAGEKLAEEKPATRVVLLTAAVEENQLVEALEL
                     GVGGVVLKEMAPRLLVEAVREVHGGGRWLDQGSTHRALDKLLQREANSRETARLLTPR
                     ELEIVRMVARGLRNRAIAEQLFITEGTVKIHLHNIYQKVEVDGRLELTLYAQSRGLV"
     gene            706..1755
                     /locus_tag="VEL74_22300"
     CDS             706..1755
                     /locus_tag="VEL74_22300"
                     /inference="COORDINATES: protein motif:HMM:NF016338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65330.1"
                     /translation="MILDRISRYLTDPRSAAFDELALAAFAFQYERIEPYRRLCQGRG
                     VTPETVAADWRRVPPVPTSAFKTLTLAAEAPREVFRSSGTTATEGERSVHHHPYPDLY
                     RQVIDASFPRFCLPLGAPVPMLSLVPTREQLPDSSLSFMIDHVLARYGAPQSATAFGA
                     RGVEVPKARSWAGGRQREGRPVLVLATALALAQWLDALERQDLRFRLPAGSAVFETGG
                     FKGRSTELTREDLLARLGERLHVTPRAVVREYGMTELTSQFYTRALLGGDPDLFVAPH
                     WTRVRILDPATLEEAPAGTPGLVAVFDLANLGSAVHLLTEDLGVAEGEGFRLLGRAAG
                     AELRGCSLVAEELAR"
     gene            complement(2147..3355)
                     /locus_tag="VEL74_22305"
     CDS             complement(2147..3355)
                     /locus_tag="VEL74_22305"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HYG65331.1"
                     /translation="MIRDVPTPEALASLMDRLRDGEAALRAIPAERLLDCWAETVEAF
                     LDPASPERRALDPGLARTCRLSVEGLAAAMEAVLGGVRREPAAALFAEAAAVTPAAGP
                     AAGPVLVVLAANLPALAVQPLLPALALRRPVLLKSPSAEPLFAPAFLAALARREPRLA
                     GGVAAAVWPGGDRELEAPVLERAGTVIAYGEAEAVASLEGRAPGRVVGYGPKTSLAVI
                     GSVAPDAVTEGLARDIALFDQRGCLSIAAVYTAGDAAALAESLARALADLARRWPPGP
                     AGSAALAAVQQARLEGEMRGLHHPNPRPDLPLAAGTVLVDPAPAFRPSPGLRTVRVHP
                     LEDLGSLPEILSPWSGRLQGAALAGEEARALVPRLSALGFSRFAAPGELQSPDATWHN
                     GGIHPLQALS"
     gene            3378..3878
                     /locus_tag="VEL74_22310"
     CDS             3378..3878
                     /locus_tag="VEL74_22310"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYG65332.1"
                     /translation="MEATVSHFEIPARDLERAARFYREVFGWTVEPVAWEGHPYYKVR
                     GSAKPAEGGRAGIDGGLLPADPGTPHPLLVVHVSGAPLETWLDRITAAGGTLDLPAAS
                     VGTMGVFARFRDPEGNLLGLWGPGGIGANLTLQAEKQPGQLGGPVCCGLGESPRRDGR
                     SQRPCG"
BASE COUNT          532 a         1461 c         1384 g          516 t
ORIGIN      
        1 ccgtgccctg cctgggagtc tgaatgccga tccgcctgat cctcgccgac gaccatccca
       61 tcatcctcga cgggctgcag cagctcttcc ggctggagcc cgatttcgag gtggtggcct
      121 gctgccggga cggcgaggag acgctcaagg cgctgcgcct gcacgctccc gacatcctgg
      181 tcctggacat ccggatgccg cgcggggacg gcctggcggt gctgcgggcc ctggctggtg
      241 agaagctggc cgaggagaag cccgccaccc gtgtcgtcct gctcactgcc gcggtggagg
      301 agaaccagct cgtcgaggcc ctggagctcg gggtcggcgg ggtcgtcctc aaggagatgg
      361 cgccgcgcct gctggtggag gcggtgcgcg aggtgcacgg cggcggccgg tggctggacc
      421 agggctccac ccaccgcgcg ctcgacaaac tgctgcagcg ggaggccaac agccgcgaga
      481 cggcgcgcct cctgaccccc cgggagctgg agatcgtgcg catggtggcg cgcggcctgc
      541 gcaaccgcgc catcgccgag cagctcttca tcaccgaggg gacggtcaag atccacctcc
      601 acaacatcta ccagaaggtc gaggtcgacg gacggctgga gctgaccctc tatgcccaga
      661 gccgcgggct ggtgtagctc tcgcccggtc tgacagaatc ggcgggtgat tctcgaccgg
      721 atttcccgct acctgaccga cccccggagc gccgccttcg acgagctggc gctcgccgcc
      781 ttcgccttcc agtacgagcg tatcgagccc taccgccggc tctgccaggg ccgcggcgtg
      841 acgcccgaga ccgtcgccgc cgactggcgg cgggtaccgc cggtgcccac ctcggccttc
      901 aagaccctga ccctcgccgc cgaggccccc cgggaggtgt tccgcagcag cggcacgacc
      961 gcaacggagg gggagaggag cgtccaccat cacccctacc cggacctcta ccggcaggtg
     1021 atcgacgcct cgttcccccg cttctgtctg ccgctcggcg cgccggtccc catgctctcc
     1081 ctggtcccca cccgcgagca gctccccgac tcgagcctgt cgttcatgat cgaccacgtg
     1141 ctggcccgct acggcgctcc gcagagcgcc accgccttcg gggcgcgcgg cgtcgaggtg
     1201 ccgaaggcgc gctcctgggc cggcggccgg cagcgggagg ggcggccggt cctggtgctc
     1261 gccaccgcgc tcgccctcgc ccagtggctc gacgccctgg agaggcagga cctgcgcttc
     1321 cggctgcccg cgggcagcgc cgtcttcgag accggcggct tcaaggggcg ctccacggag
     1381 ctgacccggg aggatctcct ggcccggctc ggcgagcgcc tgcacgtgac gccgcgggcc
     1441 gtggtccgcg agtacggcat gaccgagctg accagccagt tctacacccg cgccctcctc
     1501 ggcggcgacc ccgacctctt cgtcgccccg cactggacac gcgtccgcat cctcgatccc
     1561 gcgacgctgg aggaggcgcc cgccggcacc cccggcctgg tcgccgtctt cgacctggcg
     1621 aacctgggct ccgcggtgca cctgctgacc gaggatctgg gcgtcgccga aggcgaaggc
     1681 ttccggctcc tgggccgcgc cgccggagcg gagctgcgtg gatgctcgct ggtggccgaa
     1741 gagctggcgc ggtagccctc acccccctgc cccttctccc gccctccctc tccgctcctc
     1801 gggagagggg gaggcggaca ctgcgttgtg ttcccggagc gttgcgggaa cacaaacagt
     1861 gttcccggtc cactcccgga acacaaacga gccttcccgg atccttgggg aaggttcccg
     1921 gatcacaatc tcgccgggcg cggtgactct ccccccttcc ccggccgcca ttcctgttcc
     1981 ccgagtgatc cccggagcag gaataatgtt ccgggaacac tcccgagtga ttctcccagc
     2041 ctcccgaatg atcccgcgcc gggggattgt gatccgggga acacgaatag cgttccggcg
     2101 gaggcggatt gtgatccggg agagggggat tgtgttccgg ggagggctac gacagcgcct
     2161 gcagcgggtg aatccccccg ttgtgccagg tggcgtccgg ggattgcagc tcgccggggg
     2221 ccgcgaagcg ggagaagccc agggccgaga gccgggggac gagcgcccgc gcctcctcgc
     2281 cggccagcgc cgctccctgg aggcgcccgc tccagggaga gaggatctcc ggcaggctcc
     2341 cgagatcctc cagggggtgg acccgcaccg tccgcagccc cggggaggga cggaacgccg
     2401 gggccggatc gacgaggacg gtccccgccg ccagcggcag gtctggccgc gggttcggat
     2461 gatggagccc gcgcatctcc ccttcgagcc gcgcctgctg cacggcggcc agagccgcgg
     2521 agccggcggg gccggggggc cagcggcggg cgaggtccgc cagcgcccgc gccaggctct
     2581 ccgccagggc cgccgcgtcc cccgccgtgt agaccgcggc gatggacagg cacccccgct
     2641 ggtcgaacag ggcgatgtcc cgcgcgaggc cctcggtgac ggcgtccggc gcgaccgatc
     2701 cgatcaccgc caggctcgtc ttggggccat accccaccac ccggccgggc gcccgcccct
     2761 ccagcgaggc gaccgcctcg gcctcgccgt aggcgatcac cgtccccgcc cgttccagca
     2821 ccggggcttc gagctcccgg tcgccaccgg gccagaccgc cgcggcgacc cccccggcca
     2881 ggcgcggctc ccgccgggcg agggcggcga ggaaggccgg agcgaacagc ggctcggcgg
     2941 agggggactt gaggagcacc ggccggcgta gcgccagcgc cggcagcagc ggctgcaccg
     3001 ccagagccgg caggttcgcc gccagcacca ccagcaccgg gccggccgcg gggccggccg
     3061 ctggagtcac cgcagccgcc tcggcgaaca gcgccgccgc cggctcccgg cgcacgccgc
     3121 ccagcaccgc ctccatcgcc gccgccagcc cttcaacgga gagccggcag gtccgggcga
     3181 ggccggggtc cagcgcccgg cgctcgggag aggccggatc gaggaacgcc tccaccgtct
     3241 ccgcccagca gtcgagcagc cgctccgccg gaatcgcccg cagcgccgcc tcgccgtcgc
     3301 gcagccggtc catcagggag gcgagggctt ccggggtggg gacgtcacgg atcatcagag
     3361 gattatgatg cccggtcatg gaggcgacgg tcagccattt cgagatcccg gcccgcgacc
     3421 tggagcgggc ggcccgcttc taccgggagg tcttcggctg gacggtggag cccgtcgcct
     3481 gggaaggaca tccttattac aaggtgcgcg gctcggcgaa gccggcggag ggggggaggg
     3541 cggggatcga cggcggcctc ctgcccgcgg acccgggcac tccgcaccct ctcctcgtcg
     3601 tccacgtctc cggcgccccg ctggagacct ggctcgaccg gatcaccgcc gccggcggca
     3661 ccctcgacct ccccgccgcc tccgtcggca ccatgggcgt gttcgcccgc ttccgtgatc
     3721 cggaggggaa cctcctgggg ctgtgggggc cagggggcat aggcgctaac ttaaccctcc
     3781 aagctgaaaa gcagcctgga cagcttgggg ggcctgtttg ctgcgggctc ggggagagtc
     3841 caagacgtga cgggcgatct cagaggcctt gcggatgagc tcggtgaagc tca
//
LOCUS       DASPJI010000795         3234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_109127, whole genome shotgun sequence.
ACCESSION   DASPJI010000795 DASPJI010000000
VERSION     DASPJI010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109127"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3234)
                     /locus_tag="VEL74_22315"
     CDS             complement(<1..>3234)
                     /locus_tag="VEL74_22315"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65333.1"
                     /translation="EDGFFDMGGHSLLATRVLARIRQVLEVDLPLRAFFEHPTLAGLA
                     RAVERGRSAANGLSIPPLARREDVAGPAPLSYAQQRLWFLDRLMPGNPFYNLSGGMRL
                     IGPLNLAALRRSLREVGRRHETLRTRFAEVEGQPVQRIEPGLETGLPLVDLTGLPAGR
                     RDEAVLAAGVVSGSRPYDLTRPGLLRAALLRLDGESTDHVLLFGMHHIVSDAWSMGIL
                     VREVAALYAAFSQERPSPLPELPIQYADFAVWQRQWLTGDVLERQLRYWRERLTGTPG
                     TELPSDRPRPAVQTFRGGRWRTSFPQELAASLSSLARRNDASLFMVLLGAFKALLGRY
                     SGEEDIVIGSPIANRTHSGLESLIGFFVNTLVLRTDLSDDPGFLGLLERVKETSLGAQ
                     AHQDLPFEQLVEELQPQRDLSRNPLFQVMFNLLNVPASRLELSELAMEPLPQPTETSL
                     FDLQAYVMETGAGLEISWEYSSDLFERSTIERFSAHYRTLLGGIAAAPEQRLSELPVL
                     PEAERQQVLAVWNDTALDYPGICAHQLIEAQVRRSPQAVALRVEGREVLTYAELNARA
                     NRLARRLRELGLIPGALAAVALERSTELVVALLAVWKAGGVYVPLDPTHPAERLAYVL
                     EDSGASVLLRRPGADLPVREGLVVVEVGASEEGDGGDLTPLAVPEALAYVIYTSGSTG
                     RPKGVEVRHGGLVSFLTSMRREPGLGSSDVLLAVTTVSFDIAGLELYLPLAVGAQVLL
                     ASHETAMDGVRLAAALAGATVLQATPATWRLLLESGWQGTPGLKALCGGEALPRDLAR
                     EISARVGSLWNLYGPTETTIWSMLRRVDDELPETAGTGVSIGRPIANTSVHLLGRHLE
                     LVPMGAVGEICIGGAGLARGYLGRPELTAERFEPDPFASSGGARLYRTGDLGRRLLGG
                     EIEFRGRVDHQVKVRGYRVEPGEIEAALVEQPSVRQAVVVARQDELADARLVAYLVCE
                     PGSAVSQNAFREALASRLPSYMVPSVLMELDSLPLTPSGKVDRKALPRPDALPQGERS
                     APRTPTEIALAELWSHVLGVARVSVRDDFFELGGHSLLAVQAVTRVRE"
BASE COUNT          539 a         1226 c         1044 g          425 t
ORIGIN      
        1 ctcgcgcacg cgcgtcacgg cctgcaccgc gagcagcgag tgaccaccga gctcgaagaa
       61 gtcatcacgc acgctcaccc gcgccacccc gagcacgtgc gaccacagct cggccagcgc
      121 gatctccgtc ggtgtccgcg gcgccgaccg ctcaccctgc ggcaaggcgt ccggacgggg
      181 cagcgccttg cggtccacct tgccactggg agtcagcggc aacgagtcca gctccatcag
      241 aaccgacggc accatgtagg acggcagccg cgaggcaagg gcctcccgaa acgcattctg
      301 cgagaccgcg gagcccggct cgcataccag gtaggccacc agccgggcat ccgccagctc
      361 gtcctggcgt gccaccacca cggcctgccg caccgagggc tgctccacca gtgccgcctc
      421 gatctccccg ggctccaccc ggtatccgcg gaccttgacc tgatggtcga cccggccccg
      481 gaactcgatc tcccctccga gcaggcgccg ccccagatcg cccgtccggt acagtctcgc
      541 tccaccggac gacgcgaagg gatcgggctc gaaccgctcc gccgtcagct ccggccggcc
      601 caggtagccc cgggccaggc ccgcgccacc gatgcagatc tctccgaccg cacccatcgg
      661 caccagctcc aggtggcgac ccagcaggtg gaccgaggtg ttggcgatcg gccggccgat
      721 cgaaaccccg gtgccggccg tctccggcag ctcatcatcc acccgccgca gcatcgacca
      781 gatcgtcgtc tccgtgggtc cgtagaggtt ccacagcgag cccacccggg ccgagatctc
      841 ccgcgccaga tcgcgtggca acgcttcacc gccgcagagg gccttcagcc ccggagtgcc
      901 ctgccacccg ctttccagca gcagccgcca agtggccgga gtggcctgga gcaccgtggc
      961 ccccgccagc gccgccgcca gacgcacgcc gtccatcgcc gtctcatggc tcgccaggag
     1021 cacctgggct ccgacggcca gcggcaggta cagctccagt ccggcgatat cgaaggagac
     1081 cgtggtcacc gccagcagca cgtccgagga gcccagacca ggctcgcgcc tcatggaggt
     1141 caggaagctc accagccctc catggcgcac ctccactccc ttaggacggc ccgtcgagcc
     1201 cgaggtgtag atgacgtagg ccagagcctc cggcaccgcc aacggcgtca gatcgccgcc
     1261 gtccccttcc tcgctggccc cgacctccac caccaccagg ccctcgcgga ccggcagatc
     1321 cgcccccgga cgcctcagca gcaccgaagc accgctgtcc tccaacacat aggccaggcg
     1381 ctccgccgga tgggtcgggt ccagcgggac atagacgcct ccagctttcc agaccgccag
     1441 gagcgccacc accagctccg tcgagcgctc caacgccacc gccgccaaag ctcccgggat
     1501 gagtcccagc tcacggagac gccgcgccag acggttggcc cgggcgttca gctccgcata
     1561 ggtcaggacc tctctcccct cgacccgcag cgccaccgcc tggggcgagc gccgcacctg
     1621 ggcttcgatg agctggtgcg cgcagatgcc gggatagtcc agggccgtgt cgttccacac
     1681 ggccagcacc tgctgccgct cggcctccgg cagcactggc agctcggaca ggcgctgctc
     1741 cggcgccgcg gcgatccctc cgagaagagt ccggtagtgg gccgagaagc gctcaatggt
     1801 ggagcgctcg aacaggtccg agctgtactc ccaggagatc tccagacccg cccccgtctc
     1861 catcacgtag gcctggagat cgaacagtga cgtctcggtc ggctgcggca gcggctccat
     1921 cgccaattcc gagagctcga ggcgggaagc cggcacattg agcaggttga acatcacttg
     1981 gaagagcggg ttgcgcgaca ggtcgcgctg cggctgcagc tcctccacca actgctcgaa
     2041 cggcaggtcc tggtgcgcct gggcgccaag gctggtctcc ttgacccgct ccagaagccc
     2101 caggaaacct ggatcatctg acaggtcggt ccgtagcacc agggtgttga caaagaagcc
     2161 gatcaggctc tccagaccgc tgtgggtccg gttcgcgatg ggcgagccga tcacaatgtc
     2221 ttcctcgccg ctgtagcggc ccagcagggc cttgaaggct cccaagagga ccatgaacaa
     2281 ggaggcgtcg ttccgccgcg ccaggctcga caaggacgcg gccagctcct gggggaaaga
     2341 ggtccgccag cggccaccgc ggaaggtctg gacggccgga cgcggccggt cggacggcag
     2401 ctccgtgcct ggcgttccag tcagccgctc gcgccagtag cgcagttggc gctccagcac
     2461 gtcgccggtc agccactgcc gctgccagac cgcgaagtcg gcgtactgga taggcagctc
     2521 gggcagcggc gaggggcgtt cctgcgagaa cgccgcatac agggccgcca cctcccgcac
     2581 caggatcccc atggaccagg cgtcggagac gatgtggtgc atgccgaaga gcaggacgtg
     2641 atcggtgctc tccccgtcga gacgcagcag ggcggcgcgc aggaggccgg gccgggtgag
     2701 gtcgtacggc cgcgatccgc tcaccacccc ggcggcgagc accgcctcgt cccgccgccc
     2761 ggccggcagc ccggtcaggt cgaccagcgg caggccggtt tcgaggccgg gctcgatccg
     2821 ctgtactggc tggccttcca cctcggcgaa gcgggtgcgt agcgtctcgt ggcgccgccc
     2881 cacctcgcgg aggctccggc gtagcgcggc cagattcaga ggcccgatca ggcgcatccc
     2941 gcctgacagg ttgtagaagg ggttccccgg catgagccgg tccagaaacc agagtcgctg
     3001 ctgcgcgtag gagagcggcg ccggaccggc tacgtcttcc cgtcgcgcga gcggggggat
     3061 cgacaatccg ttggccgcgc tcctcccccg ctccaccgcc cgtgccaggc ccgcgagcgt
     3121 gggatgctcg aagaacgcgc gtagcggcag gtccacctcc agcacctggc ggatgcgcgc
     3181 caggacccgt gtggccagca acgagtgccc gcccatgtcg aagaagccgt cctc
//
LOCUS       DASPJI010000796        15359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_565607, whole genome shotgun sequence.
ACCESSION   DASPJI010000796 DASPJI010000000
VERSION     DASPJI010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15359)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15359
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565607"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="VEL74_22320"
     CDS             complement(<1..1596)
                     /locus_tag="VEL74_22320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG65334.1"
                     /translation="MFQPIKVVDLELSRPLPDLTGLERYRAVEALVRLRGRPVASLRV
                     PVEAGGCTAAALERALFQQAAGPLLTALLAAEMENPPPDLPHSLPHALSGGEAPPSVS
                     SLPLVTIAVCTRDRTEDLAACLAALERMEYPALELLVVDNAPTSDATERLVRERHPRV
                     RYCREPRPGLDWARNRAIREAQGEIVAYTDDDVRVDPGWAGAIARVFADHPEVAAVTG
                     LVAPFELETEAQVLFESYGGFGRGCRRLWFHVPPVRGGGPHISHHGAGRFGTGANMAY
                     RRSLFDRIGPFDPALDVGTVTNGGGDLEMFFRVLQSGHTLVYEPEALVRHRHRPGYDK
                     LRQQIANNGVGFYSFLVRTALAYPGERAKCLRFGVWWFGWWSLRRLAKSFLRPGGFPR
                     DLILAELWGSLAGLPRHRRARRRAAEIARETGEGLPPLKAPQAPPLALRPMAVRMVDC
                     DRLAPLDDVGGYQAVRVFFTRRGRLLGYVDIHHYGEPVSAARLRDEIAPRATDALLGE
                     AWERGLEEVCREMLAPFREGASPS"
     gene            complement(1602..2417)
                     /locus_tag="VEL74_22325"
     CDS             complement(1602..2417)
                     /locus_tag="VEL74_22325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG65335.1"
                     /translation="MPYSAAIDRSSPRSSEPTAAPVPQEPALVCRGVGKTFYYYEHRT
                     TSLRELFRRHLTRQPVHVRRPSFALADLSFSIGRGESVALIGLNGSGKSTVLRLLAGI
                     YEPTAGEIETRGRVGTVIELGAGFHPELTGSENVQLYAAMLGLGRREVAARFPGIVEF
                     SGVGDFIDTPVKYYSSGMQARLAFAVTMAVEPDILLLDEVMAVGDQEFRGRCLERLER
                     FRAGGGTLVLVSHDLDGLSGLCTRGLWLEGGRVRMDGGIDEVVAAYLGGEEAT"
     gene            complement(2371..3189)
                     /locus_tag="VEL74_22330"
     CDS             complement(2371..3189)
                     /locus_tag="VEL74_22330"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG65336.1"
                     /translation="MISLAPSRVSSRRRPSPLVEVWRARELLRNLVLRNLRVKYQRSF
                     LGFVWTLINPLLTIAVLVGVFGFVVRLDVPDYWAFLIAGYFLWSFAAQTLSAATAVLP
                     EHAHLARSVPFPAEVLVLAAALSRLVEFLTEMVLTVILLAVFLHHGVPLSFLWLPVLV
                     LVALVLVVGLSLPIATFSALFHDVQHTVPIAMMILFYASPVFYPAAMVPESVRPLYLL
                     NPFAGLLTLARTVLYEGRAPDLGLLASTVLVTALIAWFGHAVFRRYRPVLAEIV"
     gene            complement(3186..4178)
                     /locus_tag="VEL74_22335"
     CDS             complement(3186..4178)
                     /locus_tag="VEL74_22335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG65337.1"
                     /translation="MSGIGLSAAICTRNRPDLLRRALASLQSQSQPPEEILVVDNGPE
                     DAGEHGGRTRSLVMDEFPAVRYLAEPVAGLDFARNRALHEARGQVVAFLDDDAVAGPE
                     WAAALLRPFQEDPRVGAATGRVEALALETEAQRLFEANGGFSRGTGRVRLPHDARRPL
                     HGRRAPLIAWAVSVGSGCSLAVRRETLLALGGFDEALDLGPALPGGGDHDALWRLLEA
                     GFEVVYEPTAAAFHEHRRELPAAFDQIVGHQRALVAFLVKSLRRTGGRRRLDLLAFLA
                     WRLGKPAVRLARRAAGRDPLPAPVLLRMLAGCWRGLWAYPEAQRVAAGRREGRA"
     gene            complement(4199..5185)
                     /locus_tag="VEL74_22340"
     CDS             complement(4199..5185)
                     /locus_tag="VEL74_22340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG65338.1"
                     /translation="MSQPPEASLPFSTAFSRVSVVIPARNEEPRIGQTVGAVFDQAPE
                     GVELEVVVADDGSTDDTAAVASGAGARVVPVRSPGEPGSPGAARNRGAAAATGDPIIF
                     LDADCIPGPGWLAALLAAHAAGETVVGGALDAPDDLPFTGRCDHYCGSYHVHPGRPAG
                     YVPNHTPANLSVRRDAFLATAGFTEQGPVADGHEELRWQGQLRRTGTRIRFEPAALVH
                     HHNRTGVGNLLRRHFRWGYSSVEGKAETGTSRWNLLYRHPRLLIAAGLPLAFAHTFHT
                     LGCWLRAGKVEPVAMLPLLLTAHLSYAAGLTLGGWRWLRRRGTGTAPVPRWR"
     gene            complement(5169..6278)
                     /locus_tag="VEL74_22345"
     CDS             complement(5169..6278)
                     /locus_tag="VEL74_22345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG65339.1"
                     /translation="MCQLVRALSQLAYDVDLLTYPVGRTIEIPRVRYFRSANPFGLRR
                     VPVGLSVAKILLDVSLIAELARRLSSGTYACIHAVEEAAFPAVLLGKRFRVPVIYDMQ
                     SSLPEQLAQYLPFRGPVQVALRRVERWLLRRADSVVSSAGLAARVRAAVPGVRLREWQ
                     FAASLPAASQEEGATLRAKLGIAPERRVVLYTGTFEGYQGLPELLAAIPLVRAAVPEV
                     TFVLVGAEESGGTTIERGHVELVRSGVLRVVRRQPRERIPHYLALADVVVSPRSHGDN
                     LPLKIFDYLAAGKPIVATDIPAHRSALDESRALLTGAWSPDIARAIVRLLREPELAAR
                     LGAAGRAHAEERFGWFTFVRSVGELYKEVNGVAAA"
     gene            complement(6572..9934)
                     /locus_tag="VEL74_22350"
     CDS             complement(6572..9934)
                     /locus_tag="VEL74_22350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HYG65340.1"
                     /translation="MAKAGIDRPFVPSGFFAFRTPLLPFEELEALSEGLAAASALDDP
                     ARLEEAVAADKERLRARIRELLDRPEVQEAVFLASPSLFDGLDLWRSQPDSKKGQRAE
                     RALVRYLFRMAARATPFGLFSGCSLGPVDESAPTHLNVVPRSAYERHTRLDMDYLFAL
                     CEDLGQNPEIREELLYRPNSSLYRAAGRLRYAEARLDRKVRTHHLVAVDGTEYLEDTL
                     RRAANGARARDLAQALVDSDPDGEITFEEAREYIDELIDSQLLVSDLWPTVTGPEAIH
                     DLIAQLQSLSAPIAQEAAARLIRARDTLEKLDASGAGSSPEPYRELAKDLEALPTPVE
                     MARLFQLDMVKAAENPALGGAVLEEIMRGVEVIQRLTSRGRQEAIERFRSDFTERYGD
                     GREVPLVEVLDEEIGIGFERATGAGAEASPLLQGLPLAPPAADPTVPWGQRQGTLFRL
                     YVDALSRGRQEVQLTEADLDRLAPPPRPGSGEDQQSPLPRAFQVMATIAAESGEALNR
                     GEFQVQVEGVGGPSGARLLGRFCHADPNLHRLVEEHLRAEEALRADAVFAEVVHLPQG
                     RIGNILSRPVLREYEIPYLGRSGAPPEKQIPITDLLVSVSGGRIVLRSRRLGKEVIPA
                     LTSAHNFWTGSLGMYRFLCILQSQGVSAGGGWTWGPLDGAPFLPRVTFGRILLSRATW
                     YVSQEELKELAKTQGAARFAAVRSWAAERRLPRLIALADGDNELLVDLDNFLSVDTFV
                     DVIEGRDRVKILEFFPTPEQLWARGPEGRFVHELVIPFVRVPQQAPSDAQVSAAITAP
                     GRAPVSLEGYPRTFAPGSEWLFAKLYTGTSTADRVLVEAVAPVVRQALGSGAADGWFF
                     IRYSDPGWHVRVRFHGDPAALQSQVVPALQEAVAPMLADGRLWQVLYDTYQREVERYG
                     GPEGMPIAERLFQVDSEAVLAILEMIEGDEGTDARWRLALRGADMLLSDLGLQDEAKL
                     AVLGQMRESFGREFGMGKGLRVQLDQKFRAERKSLDALLDPANDETSPLAPGLMVLHR
                     RSQANAPLVAELERLEAEGRLTVSRAQLAPSFVHMHVNRMIRSAARAHELAIYDLLYQ
                     LHQSRAARARGKSAGKPAKGAPKAESVAAETA"
     gene            complement(10005..11549)
                     /locus_tag="VEL74_22355"
     CDS             complement(10005..11549)
                     /locus_tag="VEL74_22355"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HYG65341.1"
                     /translation="MAKPLLTGQEREAVEAISKLVYCNPFTPERITLERQVLGEAFRP
                     FDVVWHSQVDLAGSNPNVPGIQKAAEELAAELRARLAMAKKAVAAEELDLYRDLVFYH
                     LFSRCQLPFYDAIHGRDGEGAQASFGFYRDFVAGLEHFLDLPAFRDSPQDPAHLFACF
                     FQIRRAFNFTFRSIVGGSPPAARLREQVWQSIFTHDLRRYQQSLYRALGDVSTLVTGP
                     SGTGKELVARAIGYSRYIPFDPANLRFRESWAKSFYPLNLSALSPTLIESELFGHVRG
                     SFTGAVAERTGWLEVCPPLGTVFLDEIGEIEPAIQVKLLRVLETRSFQRLGDTKPRRF
                     EGKVVAATNRDLAREMQAGRFRADLYYRLCSDILVTPSLRERIAAAPEELSNLVLFLA
                     RRTVGDELAAVVAREVEGWIRENLGPGYPWPGNVRELAQCVNNVLIRREYRPASPGTA
                     GVRERLAEEILAADLSSEEVLRRYCTLVYARTGSYEEAGRRLGLDRRTVKAKVDAALL
                     AELRGF"
     gene            11726..13771
                     /locus_tag="VEL74_22360"
     CDS             11726..13771
                     /locus_tag="VEL74_22360"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HYG65342.1"
                     /translation="MKSPAIRLLVVLALLVPFHAHAAGLLIADGGFGGVLEIQEHTVQ
                     VTINNGIAVTEVTQVFRNTEDRQVEALYTFPVPKGASVANFSMWINGKEMVGEVVEKE
                     RARQIYNSYKEQRKDPGLLEQADYKTFEMRIFPIGPRAEQRVQITYYQELDFDHDWAT
                     YVYPLATVTETAVNSRVSGTFGLTLEVKSEIPITAMESPSHADDVAFARHGNDHWQAS
                     LEAEGGDLSRDVVLAYHTARPRTGVDLIASREPGEDGYFLLTLTAGEELSKAVEGMDY
                     VFVLDVSGSMNEEGKLALSRGSVSAFIEALGEGDRFDVIAFNVDARELFRELRPAETK
                     TRSEAVTFLESQKARGGTFLEPALRSAYRYKASDRPLNVVILSDGMTEQAERRTLLRL
                     IGERPEGVRVFAIGVGNEVNRPLLEQLAEDAGGLAAFVSREDNFERQAQAFRRKLLRP
                     LASNLEITLDGADAYDLEPGRLPNLYHGAPVRLYGRYREAGPVKVRIRADIGGEILDR
                     TVDIQIPEDADNPEIERMWAWHRVQRLLKEADREGNRSAVADEIVRLGEAFSIVSEYT
                     SFLVLENDAEYRRWQIERRNANRVERDRRKQGRVRAELERLRVKAVEGIGPVERKKDT
                     TASAPVLPQRLASADPPTETGPSTSGDVDFGSGAVDPLTAGLAAALAGLALARRRRQ"
     gene            13780..14391
                     /gene="rrtA"
                     /locus_tag="VEL74_22365"
     CDS             13780..14391
                     /gene="rrtA"
                     /locus_tag="VEL74_22365"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HYG65343.1"
                     /translation="MAGGRLPGVSLLLALPALVIYCLPALAARLELDWRAVSGGEVWR
                     IVTGHWTHVSLDHLLWDVAAFAILATACELRESCGRRRTLMTVGLAAVAIPITLWLAL
                     PGMIRYRGLSGIDSALFVLLAVTILKEELAAGRRGRVWVGALALAAFGAKLGFEAITG
                     TALFTEASGGAVPVPLAHLVGGLCGLAIAAMPYTPRRSPGPSR"
     gene            complement(14348..15136)
                     /locus_tag="VEL74_22370"
     CDS             complement(14348..15136)
                     /locus_tag="VEL74_22370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYG65344.1"
                     /translation="MRDRRVDGKVALVTGAASGIGRAAVLTLARHGASVLCADLAVEG
                     AERAAAEIRDAGGAASACRLDVTVERDWDEALERVRSSLGPLDILVNSAGISHAQTVT
                     EMSLEEWRQVMAVNLDGAFLGARAAVRAMRNDRRSGSIINISSASGLKASPGASAYCA
                     SKAAVCMFSKAIAKECIGDGIRVNTVCPAGVRTPLWTAMPFFRDLIEKTGSEDAAFRA
                     MAEGSLHGRFAEPEEIADAILYLASDESLYVTGTDLVIDGGYTA"
     gene            15179..>15359
                     /locus_tag="VEL74_22375"
     CDS             15179..>15359
                     /locus_tag="VEL74_22375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65345.1"
                     /translation="MTIPDKIHRSKRDAWLVAVLAVAGTLSLGISVVALFEEPATGLM
                     TLAVLLVAGGFVVWTF"
BASE COUNT         2446 a         5359 c         5456 g         2098 t
ORIGIN      
        1 ggaggggctc gccccctccc ggaagggggc gagcatctcc cggcagacct cctccagccc
       61 ccgctcccag gcctcgccga gcagcgcgtc cgtggcccgg ggtgcgatct cgtcccgcag
      121 acgagcggcg ctcaccggct ccccatagtg atggatgtcc acgtacccga gcagccggcc
      181 gcggcgggtg aagaagaccc ggaccgcctg gtagccgccg acgtcatcga ggggggcgag
      241 ccggtcgcag tccaccatcc gcaccgccat gggacggagg gcgagcggcg gcgcctgcgg
      301 ggctttgagg gggggcagcc cttccccggt ctcgcgggcg atctccgcgg cccgccgccg
      361 cgcccgccgg tggcgaggga gaccggcgag cgatccccac agctcggcca ggatcaggtc
      421 gcgcgggaag ccgcccggac ggaggaagga cttggccagc cgccgcaggc tccaccaccc
      481 gaaccaccag acgccgaagc gcaggcactt ggcgcgctcc ccgggatagg cgagggcggt
      541 gcggaccagg aaggagtaga agcccacccc gttgttggcg atctgctggc gcagcttgtc
      601 atagcccgga cggtgccggt ggcgcaccag ggcctcgggc tcgtagacca gcgtgtgccc
      661 cgactggagc acccggaaga acatctcgag gtcaccgccg ccgttggtca ccgtgcccac
      721 gtcgagcgcc ggatcgaacg gcccgatgcg gtcgaacagg gaacggcgat aggccatgtt
      781 ggccccggtc ccgaagcgcc ccgcgccatg atggctgatg tggggtccgc cgccacggac
      841 cggcgggaca tggaaccaga ggcgccggca gccgcgcccg aaaccgccgt agctctcgaa
      901 cagcacctga gcctcggtct ccagctcgaa cggggctacc aggccggtca ccgccgccac
      961 ctccgggtgg tccgcgaaga cgcgggcgat ggcgccggcc cagccggggt ccacccgcac
     1021 gtcgtcgtcg gtgtaggcta cgatctcacc ctgagcctcc cggatcgccc ggttgcgcgc
     1081 ccagtccagg cccggccgcg gctcccggca atagcgcacc cgggggtggc gctcgcgcac
     1141 cagccgctcg gtggcatcgc tggtgggggc gttgtccacc accagcagct ccagcgccgg
     1201 gtactccatc cgctccagag ccgccaggca cgccgccaga tcctccgtcc ggtccctcgt
     1261 acatactgcg atggtcacta gaggtagaga cgacacggaa ggtggtgcct ccccccctga
     1321 gagggcgtgc ggtagggagt gggggaggtc aggagggggg ttttccatct ccgccgccag
     1381 cagcgcggtc agtagcggtc ccgccgcctg ttggaagagc gcccgctcca gcgcggccgc
     1441 cgtgcaccct cccgcctcca ccgggacgcg gagggaggcc acgggccggc cacgcagccg
     1501 caccagggcc tccaccgccc ggtaccgctc gagccccgtg agatcgggga ggggccgcga
     1561 cagctccagg tcgacgacct tgatcggctg gaacaccggg ctcaagtcgc ctcctccccg
     1621 cccaggtagg cggcgacgac ctcgtcgatc ccgccgtcca tccgtaccct ccccccctcc
     1681 agccacaggc cgcgggtgca gaggccggaa agcccgtcga ggtcgtggga gaccagcacc
     1741 agcgtcccgc cgccggcgcg gaaccgctcc agccgctcca ggcaccggcc gcggaattcc
     1801 tgatcgccca cggccatgac ctcgtccagg agcaggatgt ccggctccac ggccatggtg
     1861 acggcgaagg cgagccgcgc ctgcatgccc gacgagtagt acttgaccgg tgtgtcgatg
     1921 aagtcgccca cgcccgagaa ctccacgata ccggggaagc gagcggccac ctcgcgccgg
     1981 ccgagcccca gcatggcggc gtagagctgg acgttctcgc tgccggtcag ctcggggtgg
     2041 aagcctgccc ccagctcgat gaccgtgccg acgcggccgc gggtctcgat ctcgcccgcc
     2101 gtcggttcgt agatgccggc aagaagacgc aggaccgtgc tcttgcccga gccgttgagg
     2161 ccgatgagcg ccaccgactc gccgcgtcca atgctgaagc tgaggtccgc cagggcgaag
     2221 ctgggccggc gcacgtgcac gggctggcgt gtcaggtggc gacggaacag ctcgcgcagc
     2281 gaggtcgtgc ggtgctcgta gtaatagaag gtcttgccca cgccgcggca gaccagcgcc
     2341 ggctcctggg ggaccggagc ggccgtcggc tcagacgatc tcggcgagga ccggtcgata
     2401 gcggcggaat acggcatggc cgaaccaggc gatgagggcg gtcacgagca cggtcgaagc
     2461 gaggaggccg aggtccggcg ccctcccttc gtacagcacg gtgcgcgcca gggttaggag
     2521 gccggcgaac ggattgagca ggtagagcgg ccgcaccgat tccggcacca tggccgccgg
     2581 gtagaagacc ggagaggcat agaacaggat catcatcgcg atcggcaccg tgtgctggac
     2641 atcgtggaac agcgccgaga aggtggcgat gggcagcgac aggcccacca ccaggaccag
     2701 cgccaccagc accagcaccg gcagccagag gaacgagagc ggcacgccgt gatggaggaa
     2761 gacggcgagc aggatcacgg tgagcaccat ctcggtcagg aactcgacca ggcgtgagag
     2821 ggccgccgcc agcaccagca cctcagccgg gaaggggacg ctacgggcca ggtgggcatg
     2881 ctcgggcagc accgccgtgg ccgcggacag cgtctgggcg gcgaagctcc agaggaaata
     2941 accggcgatt aggaacgccc agtagtcagg cacgtcgagg cgcaccacga agccgaacac
     3001 ccccaccagc acggcgatgg tgagcagcgg gttgatcagg gtccagacga agccgaggaa
     3061 cgagcgctgg tacttgacgc gcagattgcg cagcaccagg ttgcggagca attcccgggc
     3121 acgccagacc tccaccagtg gtgaggggcg gcgccgtgag gagacgcgtg acggggccag
     3181 ggagatcaag cccgtccctc ccggcgcccc gccgccaccc gctgcgcctc gggataggcc
     3241 cagaggcccc gccagcagcc ggcgagcatg cgcagcagga ccggcgccgg cagcggatcg
     3301 cgtccggcgg cgcggcgggc gaggcgcacc gccggctttc ccaggcgcca ggcgaggaac
     3361 gccagcaggt ccagacggcg ccggccgccg gtccgccgca gcgacttcac gaggaacgcc
     3421 accagcgccc gttgatggcc gacgatctga tcgaaggcgg ccggcagctc ccgccggtgc
     3481 tcgtgaaagg cggcggccgt gggctcgtag accacctcga acccagcttc gagcaggcgc
     3541 cagagggcgt catggtcgcc gcctccgggc agcgccggtc ccaggtcgag cgcctcgtcg
     3601 aagcctccga gggcgagcag ggtctcgcgc cggaccgcca ggctgcagcc gctgcccacg
     3661 ctgaccgccc aggcaatgag cggcgcgcgc cgtccatgca gcggccggcg ggcgtcatgg
     3721 gggagccgca cccggccggt gccgcggctg aacccgccat tggcctcgaa caggcgctgc
     3781 gcttcggtct cgagggccaa ggcctcgacg cggccggtgg cggcgcccac tcggggatcc
     3841 tcctggaagg ggcggagcag cgctgccgcc cactccgggc cggccaccgc gtcatcgtcg
     3901 aggaaggcca ccacctggcc acgggcctcg tggagcgccc ggttgcgggc gaagtcgagg
     3961 cccgccaccg gctcggccaa gtagcggacc gccggaaact cgtccatcac cagtgatcgg
     4021 gtgcgcccgc cgtgctcacc agcatcctcc ggcccgttgt ccaccaccag gatttcctcc
     4081 gggggctggc tctgggactg gagcgacgcg agagcacggc ggagcaggtc cggccggttc
     4141 cgggtgcaga tggccgccga tagaccgatc ccgctcatgc gtccgcgccc acccttctct
     4201 agcgccagcg gggtaccggc gccgtccccg tgccccgccg ccgcagccag cgccagccgc
     4261 cgagggtcag gcccgcggcg taggacagat gggccgtgag cagcagcggc agcatggcga
     4321 ccggctcgac cttcccggcc cgcagccagc agcctagggt gtgaaaggtg tgggcgaagg
     4381 ccagggggag gccggcggcg atgagcagcc tggggtgccg gtagaggagg ttccagcggc
     4441 tggtgccggt ctccgccttg ccttcgacgg agctgtagcc ccaccggaag tgccggcgaa
     4501 ggaggttccc gaccccggtc cggttgtggt ggtggaccag cgccgccggc tcgaaccgga
     4561 tgcgcgtccc cgtcctccgg agctgtccct gccagcgcag ctcctcgtgg ccatccgcca
     4621 ccgggccctg ctcggtgaag ccggcggtgg cgaggaacgc gtcgcggcgg acgctcagat
     4681 tggccggcgt gtggttgggc acgtagcccg cggggcggcc cgggtggacg tggtagctgc
     4741 cgcaatagtg gtcacagcgg ccggtgaagg ggaggtcgtc cggcgcgtcc agcgctccgc
     4801 cgaccacagt ctcaccggcg gcgtgggcag cgaggagcgc ggcaagccag cccgggcccg
     4861 ggatgcagtc ggcgtcgagg aagatgatgg gatctcccgt cgcggcggcg gctccgcggt
     4921 tgcgcgccgc gccgggcgag ccgggctccc cgggagagcg caccggcacg acccgggcgc
     4981 cagcgccgga ggcgaccgcc gcggtgtcat ccgtcgaccc atcatcggcg acgacgacct
     5041 cgagctccac accctcgggc gcctgatcga agacggcgcc caccgtctgc ccgatccgcg
     5101 gctcctcgtt ccgggcgggg atcaccacag agaccctgga aaaagcggtg gagaagggga
     5161 gggaggcttc aggcggctgc gacaccgttg acctccttgt acagctcgcc taccgagcgg
     5221 acgaaggtga accagccgaa cctctcctcg gcgtgggcgc gccccgccgc tcccagccgc
     5281 gccgccagct ccggctcgcg cagcaggcgc acgatggcgc gggcgatgtc cggcgaccag
     5341 gcgccggtga ggagcgcccg gctctcgtcc agcgccgagc ggtgggcggg gatgtcggtg
     5401 gcgacgatcg gcttgccggc ggcgagatag tcgaagatct tgagcggcag gttgtcgcca
     5461 tgggagcggg gggagaccac tacgtcggcc agggcaagat agtgggggat gcgctcccgc
     5521 ggttggcgcc tgaccacccg caggacgccg ctgcgcacca gctccacatg cccccgctcg
     5581 atggtggtcc cgcccgactc ctcggctccc accaggacga acgtcacctc cggcaccgcg
     5641 gcccgcacga gcgggatagc ggccagcagt tcgggcagcc cctggtagcc ctcgaaggtg
     5701 ccggtgtaga gcaccacccg ccgttccggc gcgatgccca gcttggcccg cagagtcgcc
     5761 ccctcctcct gcgaggccgc cggcagggag gcagcgaact gccactcccg cagccgcacc
     5821 cccggtaccg ccgcccgcac ccgggcggcc aagccggcgc tgctcaccac cgagtcggcc
     5881 cggcggagga gccagcgctc cacccgccgg agcgcgacct ggaccggccc gcggaacggc
     5941 aggtactgag cgagctgctc gggaaggctc gactgcatgt cgtagatcac cggcacccgg
     6001 aaccgcttcc ccaggagcac cgcagggaac gctgcctcct cgaccgcgtg gatgcaggcg
     6061 taggtcccac tcgacaggcg gcgggccagc tccgcgatca aggagacgtc gagcaggatc
     6121 ttcgcgaccg acaaccccac gggcacccgc cgcaagccga aaggattggc cgaacggaag
     6181 taacggactc gggggatttc gatcgtgcgt ccgaccggat aggtcagcag gtccacgtca
     6241 taggcgagct gcgacagcgc ccgcacgagc tggcacacgg cgatcggcgt tccgcgatcc
     6301 tcgtagaacg gctggggggc gacgaccagg actcgagatc cggacagatg gattcctttc
     6361 ggcaaatgcc gagacggcgg agacgaggga ctgaatgaag caggatgtgt gccacagccc
     6421 ggagagatcc ggcacgcggg ttctctcgtg aaatcaaggg gaccgcgaga gagacttatt
     6481 ggagtcaatc cataggcgga aatgtagttg taaagcggga cgaggggaga gaggaaagtg
     6541 catggagcaa ggggggcggc gccccccttg ctcatgccgt ctctgcggcg accgattcgg
     6601 ccttgggcgc tcccttggcg ggcttgcctg ccgacttgcc ccgggcccgg gcggcgcggg
     6661 actggtggag ctggtacagc aggtcgtaga tcgccagctc gtgcgcccgg gcggcggagc
     6721 ggatcatccg gttgacgtgc atgtggacga agctcggcgc gagctgcgcc cgcgaaaccg
     6781 tcaggcgtcc ctccgcttcc agccgttcca gctccgcgac gagcggtgcg ttggcctgcg
     6841 agcggcggtg caggaccatg agtcccggcg ccaaggggct cgtctcgtcg ttggccggat
     6901 cgagcagggc gtccaggctc ttgcgctcgg cgcggaactt ctggtcgagc tgcacgcgca
     6961 gccccttgcc catgccgaac tcccggccga aggactcccg catctgtccc aggaccgcga
     7021 gcttggcctc gtcctggagc ccgaggtcgg agagcagcat gtcggcgccg cggagggcga
     7081 gccgccagcg ggcgtccgtc ccttcgtccc cctcgatcat ctcgaggatg gcgagcaccg
     7141 cctcgctgtc gacctggaag aggcgctcgg cgatcggcat cccctcgggt ccgccgtagc
     7201 gctccacctc ccgctgataa gtgtcgtaga gcacctgcca gaggcggccg tcggccagca
     7261 tgggggccac cgcctcctgc agggcgggta ccacttggga ctgcagggct gccgggtcgc
     7321 catggaagcg cacgcgcacg tgccagcccg gatcggagta gcggatgaag aaccaaccgt
     7381 ccgccgcgcc cgacccgagc gcctgccgga ccaccggcgc cacggcttcg accagcaccc
     7441 ggtcggcggt cgaggtgccg gtgtagagct tggcgaagag ccactcggag cccggagcga
     7501 aggtgcgcgg atagccctcc agggagaccg gcgctcggcc cggcgcggtg atcgccgccg
     7561 agacctgcgc gtccgacggg gcctgctgcg gcacgcgcac gaacgggatc accaactcgt
     7621 gcacgaaccg cccctcgggg ccgcgggccc agagctgctc cggagtggga aagaattcga
     7681 ggatcttcac ccggtcgcgg ccctcgatca cgtcgacgaa cgtgtcgacc gagaggaagt
     7741 tgtcgaggtc gaccagcaac tcgttgtcgc cgtcggccag ggcgatgagg cgggggagcc
     7801 ggcgctcggc cgcccaggag cggacggcgg cgaagcgcgc cgccccctgg gtcttggcca
     7861 gctccttgag ctcctcctgg ctgacatacc aggtagcgcg ggagagcagg atgcggccga
     7921 aggtgacgcg ggggaggaac ggcgccccgt cgagcgggcc ccaggtccag ccgccgccgg
     7981 ccgacacgcc ctggctctgc aggatgcaga ggaagcggta catgcccagg ctgccggtcc
     8041 agaagttgtg ggcgctggtc agcgccggga tcacctcttt gcccagccgc cgcgagcgca
     8101 gcacgatgcg gccgcccgat accgacacca ggagatcggt gatcgggatc tgcttctcgg
     8161 gaggggcgcc cgaccgtccg aggtaaggga tctcgtactc ccgcaacacc ggccgggaga
     8221 ggatgttgcc gatgcgcccc tggggcaggt ggaccacctc ggcgaagacg gcgtcggccc
     8281 gcagcgcctc ctcggcccgc aggtgctcct cgacgagccg gtggagattc gggtcggcgt
     8341 ggcagaagcg gccgagcagc cgggcgccgg acgggccgcc cacgccctcc acctggacct
     8401 ggaactcgcc ccggttcagc gcctccccgg actccgcggc gatggtggcc atcacctgga
     8461 aggcgcgggg cagcggcgac tgctggtcct cgccagaccc gggacggggt ggtggcgcca
     8521 gccggtcgag gtcggcctcg gtgagctgga cctcctgccg gccgcggctg agcgcgtcga
     8581 cgtagagacg gaagagggtt ccctggcgct gcccccaggg gaccgtggga tcggcggccg
     8641 gcggcgcgag tggcaggccc tggagcagcg gcgaagcctc ggcgccggcg ccggtggcgc
     8701 gctcgaagcc gatgccgatc tcttcgtcca gcacctcgac gagcggcacc tcgcgcccgt
     8761 cgccgtaccg ctcggtgaag tcgctgcgga agcgctcgat ggcctcctgc cggccgcggc
     8821 tggtgaggcg ctggatgacc tccactccgc gcatgatctc ctcgagcacc gcgccgccga
     8881 gagcggggtt ctcggcggcc ttgaccatgt cgagctggaa gaggcgggcc atctccaccg
     8941 gcgtgggcag cgcctcgaga tccttggcga gctcgcggta gggctcgggc gaggagccgg
     9001 cgccactggc gtccagcttc tccagggtgt cccgcgcccg gatcagccgc gcggcggcct
     9061 cctgggcgat gggggcggag agggactgga gctgggcgat gaggtcgtgg atcgcctccg
     9121 ggccggttac cgtcggccag aggtcggaga ccagaagctg gctgtcgatc agctcgtcga
     9181 tgtactcccg cgcctcctcg aaggtgatct cgccgtcggg gtcggagtcc accagcgcct
     9241 gggccaagtc acgggcgcgg gcgccgttgg cggcgcggcg cagggtgtcc tccaggtact
     9301 cggtgccgtc gaccgccacc aggtggtggg tgcggacctt gcggtcgagg cgcgcctcgg
     9361 cgtagcgcag gcgcccggcg gcccggtaga ggctggagtt cggccgatag agcagctcct
     9421 cgcggatctc ggggttctgg cccaggtcct cgcagagcgc gaagagatag tccatgtcga
     9481 gccgcgtgtg ccgctcgtag gcggagcggg gaaccacatt caggtgcgtg ggcgcggact
     9541 cgtccacggg gccgagcgag cagccggaga acaggccgaa cggcgtggcc cgggcggcca
     9601 tcctgaacag atagcgcacc agggcgcgct cggcgcgctg ccccttcttg ctgtccggct
     9661 ggctgcgcca gagatcgagg ccgtcgaaca gcgaggggga ggcgaggaac accgcctcct
     9721 ggacctcggg cctgtcgagc aactcgcgga tacgggcgcg cagccgctcc ttgtcggcgg
     9781 ccaccgcctc ctcgagccgg gcagggtcgt ccagggcaga ggccgccgcg agtccctcgc
     9841 tcagggcctc cagctcctcg aagggcagaa ggggggtacg gaaggcgaag aagcctgacg
     9901 gaacgaacgg acggtcgatt cctgctttag ccacagtcgg aggaagatac cacggctccg
     9961 gaagggtttg atccgcctcc cgcccgccca catcctgccc ggtctcagaa accccgcagc
    10021 tccgctagca gcgccgcgtc caccttcgcc ttgaccgtcc gccggtcgag gccgaggcgg
    10081 cggccggcct cctcgtagct gccggtgcgg gcatagacca gggtgcagta gcggcggagc
    10141 acctcctcgg acgagaggtc ggcggccagg atctcctccg ccagccgctc cctcaccccg
    10201 gccgttcccg ggctcgccgg ccggtactcg cggcggatca gcacgttgtt gacgcactgg
    10261 gcgagctccc ggacgttgcc cggccagggg tagcccggcc ccaggttctc ccggatccag
    10321 ccctccacct cccgcgccac cacggccgcg agctcgtccc ccacggtccg ccgggccagg
    10381 aagagcacca ggttcgacag ctcctccggc gccgcggcga tgcgctcccg cagcgacggc
    10441 gtcaccagga tgtccgagca gagccgatag tagaggtcgg cacggaaccg ccccgcctgc
    10501 atctcgcggg cgaggtcgcg gttcgtcgcc gccaccacct tgccctcgaa gcggcgcggc
    10561 ttcgtgtcgc ccagccgttg gaaagaccgg gtctccagca cccgcaggag cttcacctgg
    10621 atcgccggct cgatctcgcc gatctcgtcc aggaacacgg tgccaagggg tgggcagacc
    10681 tccagccagc ccgtgcgctc cgctaccgcg ccggtgaagg agccccgcac gtgaccgaac
    10741 agctcggact cgatgagcgt cggcgacaac gccgagagat tcagcggata gaaggatttc
    10801 gcccaactct cgcggaacct gaggttcgcc ggatcgaaag gaatgtagcg cgagtagccg
    10861 atcgcccgcg ccaccagctc cttgcccgta cccgagggac cggtgaccag ggtcgagacg
    10921 tcccccaggg cacggtaaag ggactgctga taacgccgca ggtcgtgggt gaagatggac
    10981 tgccagacct gctcccgcag ccgcgccgca ggcggcgagc cccccacgat ggaacggaag
    11041 gtgaagttga atgcgcgccg gatctggaaa aagcaggcga agaggtgcgc cgggtcctgc
    11101 gggctgtcgc ggaacgccgg caggtccagg aagtgctcca ggccggcgac gaaatcacga
    11161 tagaagccga acgacgcctg ggccccttcg ccgtcccggc cgtggatggc gtcgtaaaag
    11221 gggagctggc accggctgaa gaggtgatag aagaccaggt cgcgatagag gtccagctct
    11281 tccgcggcga ccgccttctt cgccatagcc agccgggctc gcagctccgc cgccagctcc
    11341 tcggccgcct tctggatgcc cggcacgttg gggttggacc cggcgaggtc gacctgtgag
    11401 tgccagacga cgtcgaacgg ccggaaagcc tctcccagca cctgccgctc cagagtgatc
    11461 cgctccggag tgaacgggtt gcaatagacg agcttcgaaa tcgcctccac cgcctcgcgt
    11521 tcctgcccgg tgagcagcgg cttcgccata cggcccccct ttccagcatc cagcgtacag
    11581 gcactataca tcgaatgccg ggctcccgcg gctggacatt cctcgcttga tccggctctc
    11641 cctttgatct cagcggtttg cgtcggtggc ccccggatgg cacggcgctg gctctctcct
    11701 ctggcgtccc aaggagggaa cgaccatgaa atcacccgca atccggctgc tcgtcgtcct
    11761 ggcgctgctc gtcccgttcc atgcccacgc cgccggcctc ctcatcgccg acggcggatt
    11821 cggcggggtg ctcgagatcc aggagcacac cgtccaggtc acgatcaaca acggcatcgc
    11881 cgtcaccgag gtcacccagg tcttccgcaa caccgaggac cggcaggtcg aggcgctcta
    11941 taccttcccc gtgccgaagg gggcgtcggt cgccaatttc agcatgtgga tcaacggcaa
    12001 ggagatggtc ggcgaggtcg tggagaagga gcgcgcccgc cagatctaca acagctacaa
    12061 ggagcagcgg aaggaccccg gccttctcga gcaggctgat tacaagactt tcgagatgcg
    12121 catcttcccg atcggcccac gcgccgagca gcgggtacag atcacgtatt accaggagct
    12181 cgatttcgat cacgattggg ccacctacgt ctatcccctg gccacggtca ccgagacggc
    12241 cgtgaattcc agagtcagcg gtaccttcgg gctcaccctg gaggtcaagt ccgagatccc
    12301 catcaccgcc atggagagcc cgagccatgc tgacgacgtg gccttcgctc ggcacgggaa
    12361 cgatcactgg caggcgagcc tcgaggccga ggggggcgac ctgagccgcg acgtggtcct
    12421 ggcctatcac accgcccggc cgcgtacggg tgtggacctc atcgcctcgc gagagccggg
    12481 ggaggacggc tatttcctgc tgacgctgac cgccggcgag gagctgtcga aggccgtcga
    12541 agggatggac tacgtgttcg tgctcgacgt gtcggggagc atgaacgaag aggggaagct
    12601 cgccctctcc cgcggctcgg tgagcgcttt tatcgaggcg ctcggcgagg gggaccgctt
    12661 cgacgtgatc gccttcaacg tcgacgcccg ggagctgttc cgcgagctgc ggccggcgga
    12721 gaccaagacg cgctccgaag ccgtgacctt cctggaatcc cagaaggccc gtggcggcac
    12781 cttcctggag ccggctctgc gcagcgccta tcgctacaag gcatcagacc gccctctgaa
    12841 cgtggtgatc ctttcggacg gcatgaccga gcaggcggag cggcgtacgc tcctgcggct
    12901 gatcggtgag cgccccgagg gggtgcgggt cttcgccatc ggcgtgggta acgaggtgaa
    12961 ccgtccgctc ctcgagcaac tcgccgagga cgccggcggc ctcgccgcct tcgtctcccg
    13021 cgaggacaac ttcgagcgcc aggcccaggc cttccggcgc aagctcctgc gcccgctggc
    13081 aagcaacctg gagatcacgc tggacggcgc cgatgcctac gacctcgagc cgggcaggct
    13141 gccgaacctc tatcacggcg caccggtccg cctctatggc cgctatcgtg aggccggacc
    13201 ggtgaaggtg cgcatccggg ccgatatcgg cggcgagatc ctcgaccgca cggtggacat
    13261 ccagatcccg gaggacgcgg acaaccccga gatcgagcgc atgtgggcct ggcaccgggt
    13321 acagcggctt ctcaaggagg cggaccgcga gggcaaccgt agcgccgtgg ccgatgagat
    13381 cgtacgcctg ggagaagcct tttcgatcgt cagcgagtac acctccttcc tggtgctgga
    13441 gaacgacgcc gagtaccggc gctggcagat cgagcgccgc aacgccaacc gcgtggagcg
    13501 cgaccgccgc aagcagggga gggtgcgggc cgagttggag cggctgcgcg tcaaggcggt
    13561 cgaggggatc gggccggtgg aacgcaagaa ggacacgacc gcgtctgccc cggtgctgcc
    13621 gcagcgcctc gccagcgctg atcctccaac agagaccggc ccgtcgacga gcggcgacgt
    13681 ggatttcggc tcgggcgccg tggacccgtt gaccgccgga ctggccgccg ccctcgccgg
    13741 tctggcgctc gcccgccggc ggcggcaatg aaaggagaga tggcaggggg gcgcctcccc
    13801 ggcgtctccc tgctcctcgc gctgccggcg ctggtgatct attgcctgcc ggctctcgcc
    13861 gcccgtctgg agctcgactg gagggcggtc agcggcggcg aggtctggcg gatcgtcacc
    13921 ggtcattgga cccacgtctc gttggatcat ctgctctggg atgtggccgc cttcgccatt
    13981 ctggccaccg cctgcgagct gcgcgagagc tgcggccgta ggcggactct catgaccgtg
    14041 gggctcgcgg cggtggcgat tccaatcact ttgtggctgg ctctccccgg aatgatccgc
    14101 tatcgcggcc tctccggcat cgactcggcc ctcttcgtgc tgctcgccgt cacgattctg
    14161 aaagaagagc tggcggcggg ccgccgcggg cgggtctggg taggagcgct cgccctcgcg
    14221 gcgttcggag cgaagctcgg ctttgaagcc atcaccggca cggcgctctt caccgaggcg
    14281 agcgggggag ccgtgccggt gccgctggcc catcttgtgg gcggtctgtg cggtctggcg
    14341 attgctgcta tgccgtatac cccccgtcga tcaccaggtc cgtcccggtg acataaagtg
    14401 actcgtccga ggcgagatag aggatggcat cggcgatctc ctcgggctcg gcgaagcggc
    14461 cgtgcaggga gccctcggcc atggcccgga aggccgcgtc ttccgagccg gtcttttcga
    14521 tcagatcacg gaagaacggc atagccgtcc acagcggcgt ccgcacgccc gccgggcaga
    14581 ccgtgttgac gcggatgccg tcgccgatac actccttggc gatcgctttc gagaacatgc
    14641 agaccgcggc cttgctggcg cagtaggcgc tggcgcccgg cgaggctttc aggcccgagg
    14701 ccgatgagat attgatgatg ctgccgctcc ggcggtcatt cctcatggcg cggacggcag
    14761 ccctggcgcc gaggaaggcc ccatcgaggt tgaccgccat gacctggcgc cactcctcga
    14821 gactcatctc cgtgaccgtt tgggcgtggg agatgccggc cgaattcacc aggatgtcca
    14881 gcggcccgag ggaggagcgg acccgctcga gcgcctcgtc ccaatctctt tcgacagtca
    14941 cgtccagccg gcacgccgag gccgccccgc ccgcgtcccg gatctccgcc gcggcccgct
    15001 ccgcgccctc caccgcgagg tcggcgcaga gcaccgaggc gccgtgccgc gccaaagtga
    15061 gcaccgcggc gcggccgatg cccgaggccg cgccggtgac cagcgccacc ttgccatcca
    15121 cccttctgtc tcgcatttct cagctctctc tctcgcccga gcaaagtaga atgatcgcat
    15181 gacgatcccc gataagatcc atcgttccaa gcgtgatgcc tggctggtgg cggtcctcgc
    15241 ggtcgccgga acgctgtccc tcgggatctc ggtcgtggcg ctcttcgagg aaccggccac
    15301 agggctcatg accctggcgg tcttgctggt ggcaggcgga ttcgtcgtct ggacgttca
//
LOCUS       DASPJI010000797        12708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_728998, whole genome shotgun sequence.
ACCESSION   DASPJI010000797 DASPJI010000000
VERSION     DASPJI010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12708)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12708
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728998"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VEL74_22380"
     CDS             <1..679
                     /locus_tag="VEL74_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HYG65346.1"
                     /translation="VEFLHKHKLAMINQREVGSDVNSFEEGLRSALREDPDVILVGEM
                     RDYETIETALLAAETGHLVFSTLHTLDATETINRIISVFPPHHQKQIRIQLSQVLKAV
                     ISLRLVPRADGIGRVPAAEVLISTAYIRECIENKEKTKYIREQIALGTSQYGMQTFDQ
                     SLFQLYKGGLITLDEALKRATNPDEFRLKIQGVQFTADISRDQMEESLQIPGDGDLLI
                     GGSSFEI"
     gene            695..1177
                     /locus_tag="VEL74_22385"
     CDS             695..1177
                     /locus_tag="VEL74_22385"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HYG65347.1"
                     /translation="MADTTCYGKAVQLLAARSHFRAELAAKLSRRGYPQEEVEAALDR
                     LAREGFLDDTRTAREFVAHRQESRGEGRRRLAAELVRRGAPAEAVTAALSDLTPEDDL
                     EAAKEAASRWARGKSRPDAAALARHLERKGFSRRAIVAVLSERPGGGELEAWEEDPGG
                     "
     gene            1213..3879
                     /gene="alaS"
                     /locus_tag="VEL74_22390"
     CDS             1213..3879
                     /gene="alaS"
                     /locus_tag="VEL74_22390"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HYG65348.1"
                     /translation="MKSNDIRTSFSDFFRARGHRLAPSAPLVPQDDPTLLFVNAGMVP
                     FKNYFLGVEAPPAKRVVSVQKCLRVSGKHNDFENVGPSPRHQTFFEMMGNFSFGDYFK
                     EEAISAAWDLVTRVWGLPVDRLFATVYEEDDEAWDLWHRLSTLPEGRILRCGKEDNFW
                     AMGETGPCGPCSEIFVDLEPDRPQVSWAEGTESNRYLEIWNLVFMQYERDAGGGLTPL
                     PNPSIDTGAGLERTTSVLQGVRSNYDTDLFRPILDGAADLAGLRYGQDAEKDVSLRVI
                     ADHVRSVSFLLADGVIPGNTGRGYVLRRILRRAVRHGMRLGFEEPFFHKLVPVLGEVM
                     GSAYLELAATRQATVSTIKAEEESFLNTVASGTRKVQEEIERLRRAGESVLPGNMVFR
                     LYDTYGLPLELIREIGEEERFTLDEEGFKQALDEQKAQAQQATKENQNRMKALGEVIR
                     GHADLAETLFEGYDRTELPQAEVVRLATWKDGTPLRVSSLGEKQSGVVILDQTVFYAE
                     SGGQVGDTGEILWEGGKARVVDTQKDSAGVIFHFVEVEQGQLSRHTRVRLRVDHEIRL
                     ATQRNHTATHLLHAALRLVLGKSVRQAGSLVEPTRLRFDFTYHRGMFRQEQEQTEDMV
                     NEWIRRAVATETVQRSYQEAVAAGAMALFGEKYGERVRTVNIPGFSLELCGGCHVRNT
                     GEIGLFLVESERGVASGVRRIEAVTGSYALEEVRRQRAAYLELVNRLGIRATDYQQQI
                     QRAEQLRQKQNELEHELKKLRMQVVSGAPSPGDEEVLVEGIKVLAREVPPAPANEMRD
                     IADQLRSKLGSGVVVIGSRGEGTVSLVAAVSKDLTPRVKAGELVKRLSAIVGGGGGGR
                     PDFAQAGGKDPDKLPAALNAARDAVREQLAGR"
     gene            4044..4754
                     /locus_tag="VEL74_22395"
     CDS             4044..4754
                     /locus_tag="VEL74_22395"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HYG65349.1"
                     /translation="MRIGVCVSLSLSLLLGGVELRAQSAAKSTSSVKIGISPEGRKVI
                     YNEGLAHRARRTSAKLVAADPEIEPLIIRHSDAQQLDPKLVKALIQVESGYNQRARSN
                     KGAMGLMQLMPGTASDLRVQNPYDPDENLRGGTAYLRQMIDRFNGKLEWAVAAYNAGP
                     GAVERYGGIPPYNETRNYVRRVLSLYQGAAPAIGVTGVAGSLVPVVNGAGGIRRKPHL
                     IRNSQNRLVLTSSLDGVR"
     gene            4848..5399
                     /gene="pgsA"
                     /locus_tag="VEL74_22400"
     CDS             4848..5399
                     /gene="pgsA"
                     /locus_tag="VEL74_22400"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007128679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HYG65350.1"
                     /translation="MLNLPNLLSIFRILLVPPLVVVLLTKFDGKEWWGLGLFLLAAVM
                     DFLDGYLARRRQEVTRLGTLLDPAADKILVSAAFISLVELDPGVVPAWMVVVIIAREF
                     AVTSLRSFAAAESLVIPAGFSGKVKTIVQIVAIALLIIYNQLGEFRHLAPISLWVAVV
                     VTAYSGIEYFVRFARLILGSPKT"
     gene            5424..6632
                     /locus_tag="VEL74_22405"
     CDS             5424..6632
                     /locus_tag="VEL74_22405"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYG65351.1"
                     /translation="MPRSPIRFRPPALAISSEARWMLLRAFGPPSAPPPRPVDAAACL
                     ALARRFELSARVAARNGRERLAAELGAETADGFRRDQSMTAALGLRLLAVARDVAEAA
                     VPLDLPLVVFLKYVALDAVGLPVVGARSACDVDVLVPAGREGDLQRALLARGYHDSGL
                     REGEHQLPSLTHPGGGKVEVHRRMPGVRPASSRSATLEALAGAGLLSAVPGLPGRCAV
                     PVPGVLAAHALVHGLGQHGFWPDSYPLFKMVGDLADLGFADPGKLEEIAPWVARDVSR
                     EEAEAVRRLCGALVAGSDPAGWPGEEGEEVGGEMVLLRHILAGRLDPDYAAAMRLGFF
                     HSQPSDHPPLVRLLRSVVDALVLSRAQIDAIYGPPRHPLGYLGRRLARPLDLLRRLGS
                     YGIKALRVRP"
     gene            6661..8544
                     /locus_tag="VEL74_22410"
     CDS             6661..8544
                     /locus_tag="VEL74_22410"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HYG65352.1"
                     /translation="MKGARGPSRQPSRHTSRGPAAAPAAPRQSPALKLATFALLLLNL
                     VPPFLLSPTAKESFRLPKLLVSEWLGLLSLLFLAWQVRGMRTVSLAGLWRRPAIRALL
                     PMLLVATAGIWTTAHPVHLREGLIDLWIGAACLAGWSLGLSPSRLERLLTWLLWPASA
                     LALIAIVQFHDIFQPLGLVGLAYDARLALTSTAGNPGDLGAYLVLPCLVAQRLLLRRD
                     PAPHPRVVRWGAAAALGICLYALLLTQTLAALAALAAGTLVLWLHLLPRRRAAVLLAG
                     GAAVALALVLAVPPLRGRVAEKIDQVLRGDLNAALTGRLDGWYAAGWMVRQHPLTGVG
                     HGAYRPEFVPAKLALLDEGVRFYPGQVLVVFGNAHNEYLEAMAEWGWPGVAALLWALW
                     VLVAALRRPARQAAGPGETEMEEETEEAKRDRGLAWAGTISLAVLCLAYFPFRVALVA
                     FPALLFLAWVLRRGTPATPPNEAGTGGRVTGRLLVWPLLVVLALALAGQTVRWRDRML
                     GGRLLRTAELLSMRALATGGAPPQLIAVNLNALRRAADLDPVEVGIPIARGTQFLLAA
                     NPEAALDAYRQALALEPRPEIYFNLGRALHGAGRTEEARRQFEIALRLDPRLTKLLPA
                     GVR"
     gene            8562..8855
                     /locus_tag="VEL74_22415"
     CDS             8562..8855
                     /locus_tag="VEL74_22415"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HYG65353.1"
                     /translation="MSEFRPGPETRVRPRGDVLFRDLGGEAVLLDPEAGTYFGLNEVG
                     TEIWSLLAEGTPLGEAHVRLVRTYDAPADEVWEDLVALVRDLVGSRLVEVLDD"
     regulatory      9006..9083
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            9300..10775
                     /locus_tag="VEL74_22420"
     CDS             9300..10775
                     /locus_tag="VEL74_22420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65354.1"
                     /translation="MRFHRTIGKSITLLTFAVLAVLALPAYATPLAPFIDLQEKGLTL
                     VDDGDGLVGWGGGPRTLTVDVQGPVRFALLYWAGRERPCAETAPGDCSGFVQPFKDQQ
                     MVFNGTPITGTVIGTETQPVSGGGPILNVGYFADVTSLVSAAGPGLHDFTFEDGNDAS
                     NLWRLNGVGLLVAYTNSSDPKTYRVLVWDGLDFAFGPDPTPGDTRMTAPVTFNHGANS
                     ADRMARLRLFMGDGTIDRPDHVTISNNPTQFNELNGDDGAQWETDDYSILIPSGVGTT
                     TIQVFSAPSNQNPDSLLWEVAALRVEQLDAAAPTCPTQLVAGPPAQAITTFEDNGSGL
                     ASLLVTKSENADTVVPPFAVGTTDPVVVTATKIDQTKRARIEIIATDLAGNSRICDPI
                     HTLVVRNVGNNNETADLKSSDVPFAENKVTIINGSSGLATLEVKVNGQKFKASGLKDG
                     ETRTLDISSAMQPGDNNVVGLKGTGKPGSWADVFIWDGVSQ"
     gene            10788..10922
                     /locus_tag="VEL74_22425"
     CDS             10788..10922
                     /locus_tag="VEL74_22425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65355.1"
                     /translation="MNNSLGVPWAPSQSPKEEHFMKKAYRKPTLRRLGLLRQVTRFSF
                     "
     gene            11017..11295
                     /gene="pqqD"
                     /locus_tag="VEL74_22430"
     CDS             11017..11295
                     /gene="pqqD"
                     /locus_tag="VEL74_22430"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HYG65356.1"
                     /translation="MIGPDSRLRRRDRVLVQSAADQRILLDLDGGQYYALDEVSGKVW
                     ELCDGTRDVAAVVAAVCQDYDAPPEEIEEDVRAFLEELVDEKLVVAEA"
     gene            11267..11794
                     /locus_tag="VEL74_22435"
     CDS             11267..11794
                     /locus_tag="VEL74_22435"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HYG65357.1"
                     /translation="MRSWSLPKHEARPEAVPRARPEAGLLFAVRVLLFAVFAVPLLLR
                     LVKLPRLRDWLEPHLDRPAPALSDRAAVESLVGRIDALLAAGRPFVRSGCLTRGVTLY
                     YFLRRAGAGVCLVFGMGKMGGAIAGHCWLVFAGEPLVEKEDPRPLFVETWRIAPSAAP
                     AAEGERRSIAAAEAR"
     gene            11791..>12708
                     /locus_tag="VEL74_22440"
     CDS             11791..>12708
                     /locus_tag="VEL74_22440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65358.1"
                     /translation="MTRSSYDLHGLRLAINPAQASDGETMETMGALQARLASLPPADG
                     ADGGGGPPDLLFEIHTVSGGREHGLARPAAPRPVYDPPLGEVVYDDGGDILYIEHGPR
                     LRVLAEPGLGRVRVSAHVSGDTGDAGDADGSDVWLLSHPLFTVPLAELLKRRGLYSLH
                     AAGLCLDGRALVLPGTSGSGKSTLALALVRAGFGFLGDDTLFLSRKAEGLRLLAFPDE
                     LDLTDQTVGFFPELAPLLAEGARPGWRKRQLRAEEAYGAQVVWECAPGALVFPRVAGR
                     QESVLTPIDPGEALLELVPNVLLTEPASSQ"
BASE COUNT         1785 a         4636 c         4375 g         1912 t
ORIGIN      
        1 ggtcgagttc ctgcacaagc acaagctcgc gatgatcaac cagcgcgagg tcggcagcga
       61 cgtgaactcg ttcgaggagg gactgcgcag cgcactgcga gaggaccccg acgtcatcct
      121 cgtcggtgag atgcgcgact acgagacgat cgagacggcg ctgctcgccg ccgagaccgg
      181 ccacctcgtg ttctcgacgc tgcacaccct ggacgcgacg gagacgatca accgcatcat
      241 ctcggtcttc ccgccgcacc accagaagca gatccgcatc cagctctcgc aggtgctcaa
      301 ggcggtcatc tcgctgcgcc tggtgccgcg cgccgacggc atcggccgcg tgcccgccgc
      361 cgaggtcctg atctcgaccg cgtacatccg cgagtgcatc gagaacaagg agaagaccaa
      421 gtacatccgc gagcagatcg cgctcggcac cagccagtac ggcatgcaga ccttcgatca
      481 gtcgctcttc cagctttata aaggcggcct gatcacgctc gacgaggcgt tgaagcgcgc
      541 caccaacccg gacgagttcc ggctcaagat ccagggcgtg cagttcaccg ccgacatctc
      601 gcgcgaccag atggaggagt cgctgcagat ccccggcgac ggcgacctgc tcatcggcgg
      661 gtcgtcgttc gagatctagg tgccgggaat ctagttggcg gacaccacct gttacggcaa
      721 ggccgtgcag ctcctggcgg cgcggtccca cttccgggcg gagctggcgg ccaagctctc
      781 ccggcgcggc tatccgcagg aggaggtcga ggccgccctc gaccgcctcg cccgcgaggg
      841 gttccttgac gacacccgca ccgcccgtga gttcgtcgcg caccgccagg agtcgcgcgg
      901 cgaggggcgg cggcggctgg cggcggagct ggtgcgccgc ggcgcaccag ccgaagcggt
      961 gaccgcggcg ctctccgatc tcacccccga ggacgacctg gaggcggcga aggaggccgc
     1021 gtcgcggtgg gccaggggca agagccggcc cgatgccgcg gccctcgccc gccatctgga
     1081 gcggaagggg ttctcccgcc gtgctatcgt agccgttttg agcgagaggc ccggaggcgg
     1141 cgagctggaa gcctgggaag aagaccccgg cggctgagcg ttcctccgat gttcctttcc
     1201 ggcgggacac ccatgaaaag caacgacatc cgtaccagct tcagcgactt cttccgtgcc
     1261 cgcgggcacc gcctcgcccc cagcgcgccg ctggtgccgc aggacgatcc cacgctcctg
     1321 ttcgtcaacg cgggcatggt gccgttcaag aactacttcc tgggtgtcga ggcgccgccg
     1381 gccaagcgcg tggtctccgt ccagaagtgc ctgcgcgtct cgggcaagca caacgacttc
     1441 gagaacgtcg gccccagccc gcgccaccaa accttcttcg agatgatggg caacttctcg
     1501 ttcggcgact acttcaagga ggaggcgatc agcgccgcct gggacctggt cacccgcgtc
     1561 tgggggctgc cggtcgaccg gctgttcgcc accgtctacg aagaggacga cgaggcctgg
     1621 gacctctggc accggctctc caccctgccc gaggggcgga tcctgcgctg cggcaaggag
     1681 gacaacttct gggccatggg cgagaccggc ccctgcggcc cgtgcagcga gatcttcgtc
     1741 gacctcgagc cggaccggcc gcaggtgagc tgggccgagg gcaccgagtc caaccgctat
     1801 ctcgagatct ggaacctggt gttcatgcag tacgagcgcg acgccggcgg cggcctgacg
     1861 ccgctgccca acccgtcgat cgacaccggc gccggcctcg agcggaccac gtcggtgctc
     1921 cagggggtgc gctcgaacta cgacaccgac ctgttccggc cgatcctcga cggcgccgcc
     1981 gacctggcgg ggctgcgcta cggccaggac gccgagaagg acgtgtcgct gcgcgtcatc
     2041 gccgaccacg tgcggtcggt gtcgttcctg ctcgccgacg gcgtgatccc gggcaacacc
     2101 gggcgcggct acgtgctgcg ccgcatcctg cgccgggcgg tgcgccacgg catgcggctg
     2161 ggcttcgagg agccgttctt ccacaagctg gtgccggtgc tcggcgaggt gatgggctcg
     2221 gcctacctgg agctggcggc gacgcgccag gccacggtgt cgaccatcaa ggccgaggag
     2281 gagagcttcc tcaacacggt cgccagcggc acccgcaagg tgcaggagga gatcgagcgc
     2341 ctgcgccgcg ccggcgagtc ggtgctgccc ggcaacatgg tcttccggct gtacgacacc
     2401 tatggcctcc cgctggagct gatccgcgag atcggcgagg aggagcgctt caccctcgac
     2461 gaggaggggt tcaagcaggc gctcgacgag cagaaggcgc aggcgcagca ggcgaccaag
     2521 gagaaccaga accggatgaa agccctcggc gaggtgatcc gcggccacgc cgacctcgcc
     2581 gagacgctgt tcgaggggta cgaccgcacc gagctgccgc aggccgaggt ggtccgcctg
     2641 gctacctgga aggacggcac gccgctgcgc gtctcctctc tcggggagaa gcagtcgggc
     2701 gtggtgatcc tcgaccagac cgtcttctat gccgagtcgg gcggccaggt gggcgacacc
     2761 ggcgagatcc tctgggaggg aggcaaggcg cgggtggtcg acacccagaa ggactccgcg
     2821 ggggtgatct tccatttcgt cgaggtggag caggggcagc tctcgcgcca cacgcgggtc
     2881 cggctgcggg tggatcacga gatccgcctg gccacccagc gcaaccacac ggccacccat
     2941 ctgctgcacg cggcgctgcg cctggtgctg ggcaagagcg tgcgccaggc cggctcgctg
     3001 gtggagccga cgcggctgcg cttcgacttc acctaccacc gcggcatgtt ccggcaggag
     3061 caggagcaga ccgaggacat ggtcaacgag tggatccgcc gcgcggtggc caccgagacc
     3121 gtgcagcgca gctatcagga agcggtggcc gcgggcgcca tggcgctgtt cggcgagaag
     3181 tacggcgagc gggtgcgcac cgtcaacatc ccgggcttca gcctagagct ctgcggcggc
     3241 tgccatgtcc gcaacaccgg cgagatcggc ctgttcctgg tggagtccga gcgcggcgtg
     3301 gcctcgggcg tgcgccgcat cgaggcggtg accgggtcct acgcgctgga ggaggtgcgc
     3361 cgccagcgcg ccgcctacct ggagctggtc aaccgcctgg gcatccgcgc caccgattac
     3421 cagcagcaga tccagcgcgc cgagcagctc cggcagaagc agaacgagct ggagcatgaa
     3481 ttgaagaagc tgcgcatgca ggtggtctcc ggcgctccgt ccccgggtga cgaggaggtc
     3541 ctggtggagg gcatcaaggt gctcgcccgc gaggtgccgc cggcgccggc caacgagatg
     3601 cgggacatcg cggaccagct ccgctcgaag ctcggctcgg gcgtcgtggt catcggctcg
     3661 cgcggcgagg gcacggtgag cctggtggcg gcggtctcca aggacctgac gccgcgggtc
     3721 aaggccggcg agctggtcaa gcgcctctcc gccatcgtcg gcggcggcgg cggcggccgt
     3781 cccgacttcg cgcaggccgg cggcaaggac ccggacaagc tccccgccgc cttgaacgcc
     3841 gcccgcgacg cggtgcgcga gcagctcgcg gggcggtagg cctcacccac ctggggggct
     3901 ccccgccccc cagtttctca ccctattgcc aaaactctct tcatttgagc taagatacag
     3961 cccgccgctc aacgcgctca gtttgttgta tcgggatccg cggcaccgtc gttacggagt
     4021 cctgtcgtat gagatctgta tccatgagga tcggggtctg tgtctctcta tctctgagcc
     4081 tgctgctcgg cggagtggag ctgcgggccc aatcagctgc caagtcaacc tccagcgtca
     4141 agatcggcat cagccccgag ggacgcaagg tcatctacaa cgaggggctg gcgcaccgcg
     4201 cccgccgcac ctcggccaag ctggtggccg ccgatcccga gatcgagccg ctgatcatcc
     4261 gccacagcga cgcccagcag ctcgacccca agctggtcaa ggcgctcatc caggtggagt
     4321 cgggttataa ccagcgcgcc cgctccaaca agggggccat gggcctgatg cagctcatgc
     4381 cgggcacggc gagcgatctg cgggtgcaga acccctatga tcccgacgag aacctgcgcg
     4441 gcggcaccgc ctacctgcgg cagatgatcg accgcttcaa cggcaagctc gagtgggcgg
     4501 tggccgccta caacgccggc cccggcgccg tcgagcggta cggcggcatc cccccctaca
     4561 acgagacccg gaactacgtg cgccgggtgc tgtcgctcta tcagggggcc gcgcccgcta
     4621 ttggggtcac gggggtcgcg gggagcctgg tgccggtggt gaacggtgcc ggcggcatcc
     4681 gccgcaagcc gcacctgatc cgcaacagcc agaaccgtct cgtcctcacc agctcgctcg
     4741 acggggtccg gtagcgggtg caccccccga gacccggcag acggcgcgcc gggacccggc
     4801 ggtggcatac aatgccccgg tagcggcccg tccgccctct cagaccgatg ctcaatctgc
     4861 cgaatctcct gtcgatcttc aggatcctgc tcgtgccgcc gctcgtggtg gtgctgctca
     4921 ccaagttcga cggcaaggag tggtgggggc tcggcctctt cctgctcgcc gcggtgatgg
     4981 acttcctgga cggctacctg gcgcgccgcc gccaggaggt gacccgtctc ggcaccctgc
     5041 tcgacccggc ggcggacaag atcctggtgt cggcggcgtt catctccctg gtggagctgg
     5101 acccgggcgt ggtgcccgcc tggatggtgg tggtcatcat cgcccgcgag ttcgccgtga
     5161 cctcgctgcg cagcttcgcc gcggcggaga gcctggtgat ccccgcgggc ttctcgggca
     5221 aggtcaagac gatcgtccag atcgtcgcca tcgcgctcct catcatctac aaccagttgg
     5281 gggagttccg gcacctggct ccgatctcgc tgtgggtggc cgtcgtggtg accgcctact
     5341 ccggcatcga gtacttcgtc cgcttcgccc ggctcatcct gggctccccg aagacctgag
     5401 cccgaggggt atccttgggc cggatgcccc gctcgccgat ccgcttccgt ccgccggccc
     5461 tggcgatctc ttcggaggcg cgctggatgc tattgcgcgc cttcggacca ccgtctgcac
     5521 cgcccccccg acccgtagac gccgccgcct gcctggccct ggcccggcgc ttcgagctgt
     5581 ccgcccgcgt cgccgcgcgc aacgggcggg agcggctggc ggccgagctg ggggccgaga
     5641 cggcggacgg cttccggcgc gaccagtcga tgacggcggc cctggggctg cgcctcctgg
     5701 ccgtggcccg ggacgtggcc gaggcggccg tgcccctgga tctgccgctg gtggtcttcc
     5761 tcaagtacgt ggcgctcgac gccgtggggc tgcccgtggt cggggcgcgc tccgcctgcg
     5821 acgtggacgt gctggtgccg gcgggacggg agggcgatct ccagcgggcg ctcctggcgc
     5881 ggggctacca cgactcgggc ctgcgcgagg gggagcacca gctcccgtcg ctgacccatc
     5941 cggggggagg caaggtggag gtccaccggc ggatgcccgg ggtgcgaccc gcttcgtcac
     6001 ggtccgcgac cctggaggcg ctggccggcg ccgggctcct ctcggccgtc cccggcctgc
     6061 cggggcgctg cgccgttccg gtcccggggg tgctggcggc gcacgcgctg gtccacggcc
     6121 tggggcagca cggcttctgg cccgactcct atcctctctt caagatggtg ggcgacctcg
     6181 ccgacctggg ctttgccgat ccggggaagc tggaggagat cgccccctgg gtcgcgcggg
     6241 acgtctcccg ggaggaggcc gaggccgtcc ggcggctgtg cggcgccctg gtggcgggca
     6301 gcgatcccgc cggatggccg ggggaggagg gagaggaggt gggtggagaa atggtgctgc
     6361 tccgccacat cctggccggg cggctcgatc cggactacgc cgccgccatg cggctcggct
     6421 tcttccactc ccaaccgagc gaccacccgc cgctcgtccg gctcctgcgc tccgtcgtcg
     6481 acgcgctcgt cctctcccgc gcccagatcg acgccatcta cggcccgccg cgccatccgc
     6541 tcggctacct gggccgccgc ctcgcccgcc ccctcgacct cctccgccgc ctggggagct
     6601 acggcatcaa ggcgttgcgg gtccggccct gatccgggac gcctgtatac tcctcctccg
     6661 gtgaaaggag cccgcggacc ctcccgtcag ccctctcggc atacctcgcg cggccccgcg
     6721 gccgctccgg cggccccgcg ccagtccccg gccctgaagc tggcgacctt cgctctcctt
     6781 ctcctcaacc tggtgccgcc gttcctcctc tcgcccacgg cgaaggagtc gttccggctg
     6841 cccaagctcc tggtgagcga gtggctcggc ctgctgtcgc tcctgttcct cgcctggcag
     6901 gtccgcggga tgaggacggt gagcctcgcc gggctctggc gccggcccgc catccgcgcg
     6961 ctcctcccca tgctcctggt agcgaccgcc gggatctgga cgacggcgca tccggtccat
     7021 ctgcgcgagg ggctcatcga cctctggatc ggcgccgcct gcctggccgg ctggagcctg
     7081 gggctctcgc cgtcgcggct ggagcgcctg ctcacctggc tgctgtggcc cgcgtcggcc
     7141 ctggccctga tcgccatcgt ccagttccac gacatcttcc agccgctcgg cctcgtcggg
     7201 ctggcctacg acgcccgcct ggccctgacc tccacggccg gcaaccccgg ggacctcggc
     7261 gcctatctcg tgctcccgtg cctggtggcc cagcggctcc tcctgcggcg ggacccggcg
     7321 ccgcatcccc gcgttgtccg ttggggagcc gcggcggccc tcgggatctg cctgtacgcg
     7381 ctgctgctca cccagaccct cgccgccctc gccgccctgg ccgccgggac cctggtcctc
     7441 tggctccacc tgctgccccg ccgccgggcg gccgtgctgc tcgcgggcgg agcggcggtc
     7501 gccctcgcac tggtgctggc ggtgccgccg ctgcgcgggc gcgtggcgga gaagatcgat
     7561 caggtgctcc gcggcgacct gaacgcggcg ctcaccggcc gcctcgacgg ctggtacgcc
     7621 gccgggtgga tggtccgcca gcacccgctg accggcgtgg ggcacggcgc ctaccggccc
     7681 gagttcgtgc ccgccaagct ggcgctcctc gacgaggggg tccgcttcta ccccgggcag
     7741 gtcctggtgg tcttcggcaa cgcccacaac gagtacctgg aggcgatggc cgagtggggg
     7801 tggcccggag tcgccgccct gctgtgggcc ctctgggtgc tggtcgccgc cctgcggcgg
     7861 ccggcgcggc aggcggccgg tcccggggag acggagatgg aggaggagac ggaggaggcg
     7921 aagcgggacc gcgggctcgc ctgggccggc acgatctccc tggccgtgct ctgcctcgcc
     7981 tatttcccct tccgtgtcgc cctggtcgcc ttcccggccc tcctcttcct cgcctgggtc
     8041 ctccgccgcg ggacgccggc cacgcctccg aacgaggcgg ggaccggagg ccgggtgacg
     8101 ggccggctcc tcgtctggcc cctgctcgtc gttctggcgt tggcgctggc cgggcagacc
     8161 gtccgctggc gggaccgcat gctcggcggc cggctgctcc gcacggccga gctcctctcc
     8221 atgcgcgccc tcgccaccgg cggggcgccg cctcaactga tcgcggtcaa cctcaacgcc
     8281 ctgcggcggg ccgcggacct cgatccggtg gaggtgggga tccccatcgc ccgcggcacc
     8341 cagttcctcc tcgcggccaa cccggaagcc gccctcgacg cctaccggca ggcgctggca
     8401 ctggagccgc ggccggagat ctacttcaac ctcggccggg ccctgcacgg cgccggccgg
     8461 acggaggagg cgcgccgcca gttcgagatc gccctgcgcc tcgatccgcg cctcaccaag
     8521 ctgctgccgg ccggcgtccg gtgagctcca cgggacaccc cttgtccgag ttccgtccag
     8581 gcccagagac gcgggtgcgg ccccgggggg atgtcctctt ccgtgacctc ggcggcgagg
     8641 cggtcctgct ggacccggaa gcggggacct atttcggcct caacgaggtg gggaccgaga
     8701 tctggagcct cctcgccgag ggcaccccgc tgggcgaggc tcacgtccgg ctggtccgga
     8761 cgtacgacgc gccggccgac gaggtctggg aggatctcgt cgccctggtc cgcgacctgg
     8821 tgggcagccg tctcgtcgag gtcctcgacg attgaaggca tgcgcgcttt gaaaagggga
     8881 gttgtaacgt tgtaaaggga gggagggcgc tcctctcctc accggccggt ccccgccatc
     8941 cggtcctccc tccggaaccg atgaccgaag cgctggcccg ccccttgcat ctacatacaa
     9001 ctggcttgga ttcaaggcac acccgccgtg aggcggggcc gcaaagccaa gggatctcgt
     9061 cctgagatgg cccggctgcc gaaaagcccc cactgcgagg agagcaccat gaggggtttc
     9121 gggggcatca ttcgagcgtc gtcacttctc cgtccacatt ttcacccgcc acccccagtg
     9181 tctgatcagt aggcgcttct gcgccgtatt tcgaattggt tgtatcagcg cctcggtgct
     9241 gaaatgaata attgtgtcca aaccgatcgg aaacgattcg actgaggaga aaaactacca
     9301 tgagattcca tcggactatc ggcaagagca tcacgttgtt gacgttcgcc gtcctcgccg
     9361 tcctggcgct gccggcctac gccacccccc tggccccgtt catcgacctg caggagaagg
     9421 ggctgaccct ggtggacgac ggcgacggcc tcgtcggctg gggcggcggc ccgcgcaccc
     9481 tgaccgtcga cgtccagggg ccggtccgct tcgccctgct ctactgggcg ggccgcgagc
     9541 gcccctgcgc cgagaccgcc ccgggcgact gctccggctt cgtccagccg ttcaaggacc
     9601 agcagatggt cttcaacggc accccgatca ccggcaccgt gatcggtacc gagacccagc
     9661 ccgtgtccgg cggcgggccg atcctcaacg tcggctactt cgcggacgtc acctcgctcg
     9721 tctcggcggc gggccccggc ctgcacgact tcaccttcga ggacggcaat gacgcgagca
     9781 acctctggcg cctgaacggc gtcgggctcc tcgtcgccta caccaattcc tcggacccca
     9841 agacctaccg ggtgctcgtc tgggatggcc tcgacttcgc cttcggcccc gacccgaccc
     9901 cgggcgacac ccgcatgacc gcgccggtga ccttcaacca tggcgccaac tcggccgacc
     9961 ggatggcgag gctgcggctg ttcatgggtg acggcaccat cgaccggccc gaccacgtca
    10021 ccatcagcaa caacccgacc cagttcaacg agctgaacgg cgacgacggc gcgcagtggg
    10081 agaccgacga ctacagcatc ctcattccct ccggcgtggg caccacgacg atccaggtct
    10141 tctcggcccc gagcaaccag aacccggaca gcctcctctg ggaggtcgcg gccctgcgcg
    10201 tcgagcagct cgacgccgcc gcacccacct gcccgaccca actcgtcgcc ggcccgccgg
    10261 cccaggccat caccaccttc gaggacaacg gctccggcct cgcctcgctg ctggtgacca
    10321 agtccgagaa cgcggacacc gtggtgccgc cgttcgccgt gggcaccacc gacccggtgg
    10381 tcgtgaccgc caccaagatc gaccagacca agcgggcgcg gatcgagatc atcgccaccg
    10441 acctcgccgg caactcgcgg atctgtgacc cgatccacac cctggtggtc cgcaacgtcg
    10501 gcaacaacaa cgagaccgcg gacctcaagt cgagcgacgt gccgttcgcc gagaacaagg
    10561 tgaccatcat caacggcagc tccggcctcg ccaccctgga ggtcaaggtc aacggccaga
    10621 agttcaaggc cagcggcctc aaggacggcg agacccgcac cctcgacatc tcgtcggcca
    10681 tgcagcccgg cgacaacaac gtcgtgggcc tcaagggcac cggcaagccg ggctcctggg
    10741 ccgacgtctt catctgggac ggcgtgagcc agtagccggc acgcgttttg aataattcgc
    10801 tcggcgtccc atgggcgccg agccaatctc caaaggagga gcacttcatg aagaaggcgt
    10861 acaggaagcc gaccctgcgg cggctcggcc tgctgcggca ggtcacccgg ttcagcttct
    10921 gagatcctga gatgttttct gatggcggga cccgctcgtg gcgggtcccg ccagttccat
    10981 tttacgggcg gcggagcccg ctcggggagg attaccatga tcggaccgga cagccggctc
    11041 cggcggagag accgcgtgct cgtccagagc gcggcggacc agcgcatcct gctcgacctc
    11101 gacggcgggc agtactatgc gctcgacgag gtgagcggca aggtgtggga gctctgtgac
    11161 ggcacccgcg acgtcgccgc ggtggtcgcg gcggtctgtc aggactacga cgccccgccc
    11221 gaggagatcg aggaagacgt ccgggccttc ctcgaggagc tggtggatga gaagctggtc
    11281 gttgccgaag catgaggcga ggcccgaggc ggtgccccgg gcgaggccgg aagccgggct
    11341 cctcttcgcc gtccgggtgc tgctgttcgc cgtcttcgcg gtgccgctgc tgctccggct
    11401 ggtgaagctg ccgcgcctgc gcgactggct ggagccccac ctggaccggc cggcgccggc
    11461 cctctccgac cgcgccgcgg tcgagagcct ggtggggcgc atcgacgccc tgctcgccgc
    11521 cgggcgcccc ttcgtgcgct cggggtgcct cacccgcggc gtcaccctct actacttcct
    11581 gcgccgcgcc ggggccggcg tctgcctggt cttcggcatg ggaaagatgg ggggggcgat
    11641 cgccggccac tgctggctcg tcttcgcggg cgagcccctg gtggagaagg aggacccccg
    11701 gccgctcttc gtcgagacct ggcgcatcgc gccctccgcc gctccggcgg ccgaagggga
    11761 gcggcggtcg atcgccgcgg cggaggcccg atgacccgca gctcctacga cctccacggc
    11821 ctccggctgg cgatcaatcc ggcgcaggcg agcgatggcg aaacgatgga gacgatgggg
    11881 gccctccagg cccggctcgc ctcgctcccc cccgcggacg gcgcggacgg cgggggcggg
    11941 ccgcccgacc tcctcttcga gatccacacc gtcagcggcg gccgcgagca cggcctcgcg
    12001 cggcccgccg cgccgcggcc ggtctacgac ccgccgctgg gcgaggtggt ctacgacgac
    12061 ggcggcgaca tcctttatat cgagcacggg ccgcgcctgc gggtgctcgc cgagcccggc
    12121 ctcggccggg tgcgcgtgtc ggcccacgtc tccggcgata ccggcgatgc gggcgacgcc
    12181 gacggctccg acgtctggct cctctcccat ccgctgttca ccgtccccct ggccgagctg
    12241 ctcaagcgcc gcggcctcta cagcctgcac gccgcggggc tctgcctgga cggcagggcc
    12301 ctggtcctcc ccggcaccag cggctcgggc aagtcgaccc tggccctggc cctggtgcgc
    12361 gccggcttcg gcttcctcgg cgacgacacc ctgttcctct cccgcaaggc ggaggggctg
    12421 cgcctcctcg ccttcccgga cgagctcgac ctcaccgacc agacggtcgg cttcttcccc
    12481 gagctggcgc cgctcctcgc cgagggggcg cggcccggct ggcgcaagcg gcagctccgc
    12541 gccgaggagg cctacggcgc ccaggtggtc tgggagtgcg cgccgggggc gctggtcttc
    12601 ccgcgcgtcg ccggccggca ggagagcgtc ctgacgccga tcgatcccgg cgaggcgctc
    12661 ctggagctgg tgcccaatgt cctcctcacc gagccggcgt cgtcccag
//
LOCUS       DASPJI010000798         7230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_729004, whole genome shotgun sequence.
ACCESSION   DASPJI010000798 DASPJI010000000
VERSION     DASPJI010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7230)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729004"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /gene="ybgF"
                     /locus_tag="VEL74_22445"
     CDS             <1..384
                     /gene="ybgF"
                     /locus_tag="VEL74_22445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HYG65359.1"
                     /translation="EGASAPQAYYDRGYTLYHQGHYVDAEASFQRFLQASPQSELADN
                     AQYWIGECRYARGDNRGALAAFRETVERYPEGNKVPDALLKAGQSLEAMGDTDGARDS
                     YAEVARRFAGTAAAAVAEERRAKLP"
     gene            555..1739
                     /locus_tag="VEL74_22450"
     CDS             555..1739
                     /locus_tag="VEL74_22450"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HYG65360.1"
                     /translation="MRVRLSGIVLAATLLGGVTAPALADRPPQNLHMVDGHWTAWEPP
                     TPAPGTQVHVIQRGDTLWDLAARFYGNPYLWPQLWEHNRYILDAHWIYPGDPLVLGPE
                     VQPVDTLAGGAGTGELEPGEEPGEESAPVENVLTAGAAAGAPIPLGSESDLLCSGFIG
                     DAEEEFPYTIAGSEYDVLQAGLEGNAATTLQGRWGVANTVKYGLNTSDIVYIDGGRAQ
                     GLSAGTVFTAVAPGRQVIHPTGGVFGRFYNYLGRVRVLSVQETTAIAEIIHSCDPITV
                     GSLLRPYEPEPVPLARVTAMRPVNFPSPEEALENTPVIVFSEDEIISLGEDHVVYIDR
                     GAEQDVTPGDIFTIYRRNRPGLPPIVLGELAVLSVQSRSAVAKIIESRYPVYIGDRLE
                     LK"
     gene            1828..2100
                     /locus_tag="VEL74_22455"
     CDS             1828..2100
                     /locus_tag="VEL74_22455"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYG65361.1"
                     /translation="MAEQNLNGRLYQIVDELVRRGVTLEQARREFEKQFIVASLKSNR
                     GNFCRSARSLGVHRNTLRNKVSDLGIGAEEYDLPSRRPSRRKTSVT"
     gene            2336..3139
                     /locus_tag="VEL74_22460"
     CDS             2336..3139
                     /locus_tag="VEL74_22460"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HYG65362.1"
                     /translation="MKRMRLQKPFTVTAATLALVVALDTTPSDTAPSDPTRFASGFGQ
                     PQQMQELASLVLEAPTRPFHTSGMHAVTSGDLAAALDADPKPQEFELFDSERDEEARR
                     EILRDLPYGSAILRAAERHEVDGLLLAAIVEAESRFAPGAVSPRGAQGLMQIMPSTGA
                     IYGAKDLLDPYVNLDVGSRYLRSLMKDYEGDLDLVLAAYNAGPGVVQRYGGIPPYRET
                     RAYVAKVRARYTAHREAVRSSLLPEEPAAAPTHAVATAAVTMPRAIRAR"
     gene            complement(3297..3485)
                     /gene="tatA"
                     /locus_tag="VEL74_22465"
     CDS             complement(3297..3485)
                     /gene="tatA"
                     /locus_tag="VEL74_22465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HYG65363.1"
                     /translation="MGPIGIPELLIILVIVVLLFGATRLPNIGKGLGEGIRNFKKGIK
                     SDDTPERERLSERAEDRR"
     gene            complement(3532..4509)
                     /locus_tag="VEL74_22470"
     CDS             complement(3532..4509)
                     /locus_tag="VEL74_22470"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HYG65364.1"
                     /translation="MMEPLNAVAEDTDLEAWLARIGRPLRDLDRLPGDVSPRRYARLS
                     FEDGTTAILATYPPEVRAICPRFLRTTELLAGAGVRVPRVLGSACDSGWMLLEDLGPE
                     TLGDYRDRPWSELAPWFESALEAAAAIARLPVAALDGLNPTLGRELMEKELAQTWNLF
                     LEPRGLTGGPELSRALRATLNEVCANLAADPPVPCHRDFMARNLMPLPGGGVAVLDHQ
                     DLRLGPPAYDLASLLNDTVFPPPEAEEALLAAVAPGAEDRVCYHRAAAQRTIKAVGTY
                     ASFSLRGADRHLPLIPPTLARGLAHLARTPEGAPLADELARAWSGVLGG"
     gene            4717..5019
                     /locus_tag="VEL74_22475"
     CDS             4717..5019
                     /locus_tag="VEL74_22475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65365.1"
                     /translation="MLRELRRALSEAISESTEVSRTLRKIREEGYSLYLLVDCKREAD
                     EEPEPVSRSSRSRSATRTRGAAEPVFQIDGRDLSFLRSIGIDPTRRLRRRRSSPVE"
     gene            5052..6149
                     /gene="rlmN"
                     /locus_tag="VEL74_22480"
     CDS             5052..6149
                     /gene="rlmN"
                     /locus_tag="VEL74_22480"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HYG65366.1"
                     /translation="MKQTTDPKALAALPPDLLGLSRERLREELAPLIDRPFRVEQIYQ
                     ALHTRGVPDFAGMTELSKELRESLAGSFRIGLPEITHRQQSSDGTCKYLLRLPDGATV
                     EAVDIPDGDRRTFCISSQAGCALACAFCVTGYWGAGRNLTAGEIVAQVLAIRADRGLA
                     AEGLNLVFMGMGEPLLNLENLEAALGILTESISPRRITVSTVGILPGIEAIAKWERRP
                     NLAISLHAPDEERRDQVMPINRTYPLADLMPALRRYPLEKGRKITFEYILIRDFNDSV
                     RDADQLVRLLSEVRGKVNLIPINPDPVLGEKMMPPSDERIDAFQARLKQRGLVATVRR
                     RRGDDVSAACGQLRAFGRDPRGFRGRPGLAR"
     gene            complement(6150..6839)
                     /locus_tag="VEL74_22485"
     CDS             complement(6150..6839)
                     /locus_tag="VEL74_22485"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HYG65367.1"
                     /translation="MMEGMEGPVPVGGHWIWRDRHHDTQILFAGRGPDRSDERDAVLQ
                     RIAPGAPPVAWAKQIHSARVLPALPGACGEGDALVGEMGLALSVVTADCVPVVLAGPG
                     GLAAVHAGWRGIAARIVAATLDQLDGPLTQWTAWIGPAIGPCCYEVGEDVAEQVAAVS
                     GPEVILPAVHPGQRPHLDVTAAVRRQLEDAGVRDVRVISHCTLCDGERLWSYRRDGKG
                     AGRNLAFIWRG"
     gene            complement(6836..>7230)
                     /locus_tag="VEL74_22490"
     CDS             complement(6836..>7230)
                     /locus_tag="VEL74_22490"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HYG65368.1"
                     /translation="EDGLARLDEVLEHDPWLVIVELGGNDRLRQVPPERTEAALREII
                     QRLVDARVVPLVVEFDAPFSGSYNAIFGRLEEELSIPMVEDVLGDILVDSSLKSDPIH
                     PNARGHELLAEAVAEEVEELIEARKEAR"
BASE COUNT         1107 a         2629 c         2451 g         1043 t
ORIGIN      
        1 gagggggcgt cggcgcccca ggcctactac gaccgcggct acaccctcta tcaccagggg
       61 cactacgtcg acgcggaggc gagcttccag cgcttcctcc aggccagccc gcagagcgag
      121 ttggcggaca acgcccagta ctggatcggc gagtgccgct acgcccgcgg cgacaaccgc
      181 ggcgccctcg ccgccttccg ggagaccgtc gagcgctacc ccgagggcaa caaggtcccc
      241 gacgccctcc tcaaggccgg ccagtccctg gaggccatgg gcgacaccga cggcgcccgc
      301 gactcctacg ccgaggtggc ccgccgcttc gctggcaccg ccgccgccgc cgtcgccgaa
      361 gagcgccgcg ccaagctccc ctgagcccct catcctcccc gaaaaccggc tagaatcccc
      421 ggatctctca agcgctgctt gagcgatccg ggtttaaact tgacaggaca aggccggacg
      481 ctgtagctta aatcatagcg atttcactat cttttccagc ccaggcgctc aggacaggcg
      541 cccaggaggc cccgatgaga gtgcggcttt ccggtatcgt cctcgcggct acgctgctcg
      601 gtggtgtcac ggcaccggcg ttggcagatc gtccgccgca gaacctgcac atggtggatg
      661 gccattggac ggcgtgggag ccgcccacgc cagccccggg gacgcaggtc catgtgatcc
      721 agaggggaga caccctctgg gacctcgcgg cccggttcta cggcaacccc tatctctggc
      781 cccagctctg ggagcacaac cgctacatcc tggacgccca ctggatctat cccggcgatc
      841 cgctggtcct gggccccgag gtgcagccgg tcgacacgct cgccggcggc gccgggacgg
      901 gcgagctcga gcccggagag gagcccggag aggagagcgc gccggtggag aacgtgctga
      961 ccgccggcgc cgcggccggc gcgccgatac cgctggggtc ggagagcgac ctgctgtgca
     1021 gcggcttcat cggcgacgcc gaagaggaat tcccgtacac catcgccggc tccgagtacg
     1081 acgtcctgca ggcggggctc gagggcaacg cggccactac cctccagggg cggtgggggg
     1141 tcgccaacac ggtcaagtac ggcctgaaca ccagcgacat cgtctacatc gacggcggcc
     1201 gcgcgcaggg gctgtctgct ggcacggtgt tcaccgccgt cgcccccggt cgccaagtga
     1261 tccaccccac gggcggggtc ttcggccgct tctacaacta cctggggcgg gtgcgggtgc
     1321 tgtcggtgca ggagaccacg gcgatcgccg agatcatcca ttcctgcgac ccgatcaccg
     1381 tgggctcgct gctgcgcccc tacgagccgg agccggtgcc gctggcccgg gtcaccgcca
     1441 tgcggccggt gaacttcccc agcccggagg aggcgctgga gaacaccccg gtcatcgtct
     1501 tctccgagga cgagatcatc tcgctgggtg aggaccacgt ggtctacatc gaccgcggcg
     1561 ccgagcagga cgtcaccccc ggcgacatct tcaccatcta ccgccgcaac cggcccggcc
     1621 tgccgcccat cgtgctgggt gagctggcgg tcctctcggt ccagtcccgc tccgcggtcg
     1681 ccaagatcat cgagtcgcgc tacccggtct acatcggcga ccgcctcgag ctcaagtagc
     1741 accacgatga gctacccgcc cgggggtccc gcacccccgg gctgtggtat ccttagggcc
     1801 tccgaaccgc ttcttgcgag gactcccttg gccgaacaga acctcaacgg acgcttgtat
     1861 cagatcgtcg acgagttggt gcgacggggt gtgacgctgg agcaggccag gagggagttc
     1921 gagaagcagt tcatcgtggc ttcgctcaag tccaaccggg gaaatttctg ccgctccgct
     1981 cgttccctgg gcgtccaccg caacaccctc cgcaacaagg tgtccgacct gggcatcggc
     2041 gcggaggagt acgatctccc gtcccgccgg ccgtcccgcc gcaagacttc ggtcacctag
     2101 cggcgccgcc tcgagccgta gccccaaagc cgggggctcg gctggcaccg atctcgcagt
     2161 gaaaaacggc taagtctacg tgtagccacc cgaagccata gaggcttcgc ttgggtgggt
     2221 gcgctgtctt ctgaccagaa ctgcgtcctc gacccagccc ccggtcagcc aatccggcag
     2281 accggcatgc caggtccgcc gtggggaggc gaacgcagag gaggttttgg cccgaatgaa
     2341 gcgaatgaga ttgcagaaac cgttcaccgt gaccgccgcc accctggccc tggtggtcgc
     2401 cctggacacc accccttccg acaccgcccc ttccgatcct acccggttcg ccagcgggtt
     2461 cggccagccc cagcagatgc aggagctggc gtcgctcgtc ctcgaggcgc ccacccgccc
     2521 cttccacact tccggaatgc acgcggtgac ctcgggtgac ctcgccgcgg ccctcgacgc
     2581 cgaccccaag ccccaggagt tcgagctgtt cgacagcgag cgggacgagg aagcccgccg
     2641 cgagatcctg cgcgacctcc cctacggcag cgccatcctg cgcgccgccg agcgccacga
     2701 ggtggacggc ctgctgctgg ccgccatcgt ggaggccgaa tcccgcttcg cgcccggcgc
     2761 cgtctcgccg cgcggagccc aggggctgat gcagatcatg ccatccaccg gcgccatcta
     2821 cggcgccaag gacctgctcg acccgtacgt caacctggac gtggggagcc ggtatctgcg
     2881 cagcctgatg aaggactacg aaggtgacct ggacctggtc ctcgccgcct acaatgccgg
     2941 cccaggtgtg gtgcagcgct acgggggcat ccccccctac cgcgagaccc gtgcctatgt
     3001 ggccaaggtc cgcgcccgct acacggcgca ccgcgaagcc gtccgcagct cgctgctgcc
     3061 ggaggagccc gctgccgccc ccacccatgc cgtggccacc gccgcggtca ccatgccgcg
     3121 cgccattcgg gcacgctgag acgccgtacc ggcaccgacc accccccggg aaccccacgg
     3181 cccgggggga ggacccgcct caccttcgaa tctgttccgg aatgatgatg atgtagggga
     3241 ggggcttgcc ccctccccgg agggggcaag ccctccccta ccagaaaacc cggctcctag
     3301 cggcggtctt cggcgcgctc cgagagccgc tcccgctcgg gggtgtcgtc gctcttgatg
     3361 cccttcttga agttgcggat gccctcgccg aggcccttgc cgatgttggg cagacgggtc
     3421 gccccgaaaa gcaggaccac gatgacgagg atgatgagga gctcggggat gccgatgggg
     3481 cccatggggt gatctcctaa aaactctacc taccgtggcc agtctagcag atcacccgcc
     3541 gaggacgccg ctccaggccc gtgccagctc gtccgccagc ggcgccccct cgggggtgcg
     3601 agccaggtgg gcgaggccgc gggcaagcgt cggcgggatc agcggcaggt ggcggtccgc
     3661 gccgcgcaga gagaaggagg cgtacgtccc caccgccttg atcgtccgct gggccgcggc
     3721 gcggtgatag cacacacggt cctcggcgcc cggggcgacc gccgccagca gcgcctcctc
     3781 ggcctccggc ggcgggaaga cggtgtcgtt gagcagcgag gccaggtcgt aggccggggg
     3841 gccgagccgc aggtcctggt ggtcgagcac cgccacgccg ccgccgggca gcggcatcag
     3901 gttgcgcgcc atgaagtccc ggtggcaggg cacgggcggg tccgccgcca ggttggcgca
     3961 gacctcgttc agggtggcgc ggagcgcgcg ggagagctcc gggccgccgg tgagcccgcg
     4021 gggctccagg aacaggttcc aggtctgggc gagctccttc tccatcagct cgcggccgag
     4081 ggtcgggttg agcccgtcca gcgccgccac cggcagccgg gcgatggcgg ccgccgcctc
     4141 cagggcgctc tcgaaccagg gcgccagctc gctccagggg cggtcccggt agtcgccgag
     4201 cgtctcgggg ccgaggtcct cgaggagcat ccagccggag tcgcaggccg atcccagcac
     4261 tctcgggacg cgcacgccgg cgccggccaa cagctcggtg gtgcgcagga accgtggaca
     4321 gatggcccgg acctcgggcg ggtaggtggc gaggatcgca gtggtgccgt cctcgaagct
     4381 cagccgcgcg tagcggcggg gcgacacgtc gcccggcagg cggtccagat cgcgcagcgg
     4441 ccggccgatc cgagcgagcc atgcttccag atcggtatcc tcggccacgg cgttcaacgg
     4501 ctccatcatc gatacgtccc ttcgttgttc tttgctggca gacccattag cctaccacgg
     4561 cgagagccgc cgaaaaccct tgggaatggg gtccgtgccc tgtttgttga cggactcggc
     4621 gcgtgtttga cactccgcag gacgcagagg ataatccggc tcggttccgc aacgggcatc
     4681 gccggggagg ttccactgga cgagagactc aatcagatgc tgcgcgaatt gcgtcgcgcg
     4741 ctctccgagg cgatttcgga atcgaccgag gtgagccgga cgctccgcaa gatccgcgag
     4801 gagggctact ccctctacct gctcgtggac tgcaagcgcg aagcggatga agagcccgag
     4861 cccgtctccc gctcgtcccg gtcccggtcg gcgacccgga cgcgcggcgc cgccgagccg
     4921 gtcttccaga ttgacggccg cgacctctcc ttcctgcgct ccatcggcat cgaccccacg
     4981 cgccgcctgc gccgccgccg cagcagcccc gtcgaataag ctcgtcgcgg ccgatggcgc
     5041 cgctttcagc catgaagcag acgaccgatc ccaaggccct ggccgccctg ccgcccgacc
     5101 tgctcggcct ctcccgtgag cgcctccgcg aggagctcgc gccgttgatc gaccgcccgt
     5161 tccgggtgga gcagatctac caggccctcc atacgcgcgg cgtccccgac ttcgccggca
     5221 tgaccgagct gtcgaaggag ctgcgcgaga gcctcgccgg gagcttccgc attggcctgc
     5281 ccgagatcac ccaccgccag cagtcgtccg acggcacctg caagtacctg ctgcggctgc
     5341 ccgacggcgc cacggtggag gcggtcgaca tcccggacgg agaccgccgc actttctgta
     5401 tctccagcca ggcgggctgc gccctggcct gcgccttctg cgtcaccggc tactggggtg
     5461 cggggcgcaa cctcacggcc ggcgagatcg tggcccaggt gctggcgatc cgcgccgacc
     5521 gcggcctcgc cgccgagggg ctgaacctgg tcttcatggg gatgggcgag ccgctgctca
     5581 acctggagaa cctggaggcg gcgctgggca tcctcaccga gtcgatctcg ccgcggcgga
     5641 tcaccgtctc aacggtcggc atcctgccgg ggatcgaggc gatcgcgaag tgggagcggc
     5701 gccccaacct ggcgatctcc ctgcacgctc cggacgagga gcgccgcgac caggtgatgc
     5761 cgatcaaccg cacctatccg ctggccgacc tgatgccggc gctgcgccgc tatccgctgg
     5821 agaagggccg caagatcacc ttcgagtaca tcctgatccg cgacttcaac gactcggtgc
     5881 gcgacgccga ccagctcgtg cggctgctct ccgaggtgcg cggcaaggtg aacctgatcc
     5941 cgatcaaccc ggacccggtg ctgggcgaga agatgatgcc gccgtccgac gagcggatcg
     6001 acgcgttcca ggcccggctc aagcagcgcg ggctggtggc cacggtgcgc cggcgccggg
     6061 gcgacgacgt cagcgccgcc tgcggccagc tccgcgcctt cggccgcgac ccgcgggggt
     6121 tccgcggccg gccggggctc gccaggtaac tatcctctcc agatgaaggc caggttgcgg
     6181 ccggcgccct tgccgtcccg gcgatagctc cacaggcgct ccccgtcgca gagggtgcag
     6241 tgggagatca cgcggacgtc tcggacgccg gcatcctcga gctgccgccg gaccgcggcc
     6301 gtgacgtcca ggtgcgggcg ctgtccgggg tggacggcgg gaaggatcac ctcggggccg
     6361 ctgaccgcgg ccacctgctc ggcgacgtcc tcccccactt cgtagcagca gggcccgatg
     6421 gcggggccga tccaggcggt ccattgggtg aggggaccgt cgagctggtc cagggtcgcg
     6481 gcgacgatgc gggcggcgat gccgcgccag ccggcatgga ccgcggcgag ccctcccggc
     6541 ccggccagga cgacgggcac gcagtccgcg gtgacaaccg acagggcgag ccccatctct
     6601 cccaccaggg cgtccccttc tccgcaggct ccgggtaggg cgggaaggac gcgggcggag
     6661 tggatctgct tggcccaggc gaccgggggt gcgccggggg cgatgcgctg gaggacagcg
     6721 tcccgttcgt cgctacggtc gggaccccgg ccggcgaaga ggatttgggt gtcgtggtgg
     6781 cggtcgcgcc agatccagtg gcctcccacg gggacggggc cctccattcc ctccatcatc
     6841 gcgcctcctt ccgggcctcg atcagctctt ccacctcttc ggccacggcc tcggcgagca
     6901 gctcgtgccc acgggcgttg ggatggatcg gatcgctctt aagagaggag tccaccagga
     6961 tgtcgccgag cacgtcctcc accatgggga tcgagagctc ctcttcgaga cggccgaaga
     7021 tggcgttgta gctgccggag aacggcgcgt cgaactccac cacaagaggc accacccggg
     7081 cgtccacgag gcgctggatg atctcgcgca gcgccgcctc ggtccgctcc ggcggcacct
     7141 gccgcagccg gtcgttgccc cccagctcga cgatgaccag ccaggggtcg tgctccagca
     7201 cctcgtccag gcgcgccagg ccgtcctccg
//
LOCUS       DASPJI010000799        16001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_223012, whole genome shotgun sequence.
ACCESSION   DASPJI010000799 DASPJI010000000
VERSION     DASPJI010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16001)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16001
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223012"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VEL74_22495"
     CDS             complement(<1..630)
                     /locus_tag="VEL74_22495"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HYG65369.1"
                     /translation="MDLLPAIDLRHGRAVRLRQGDDARASVYGDDPVAALESYARAGV
                     ARVHVVDLDAALGEAPQRDLIAYMAASKAVAIQLGGGLRGREAVEWALGAGCDRVVIG
                     SLVARDPEAFGALAHELPGRLVPALDIEKGEVRVAGWTESARRTLADLCAGLRGLPCP
                     AVLVTDVERDGMLSGPNVELARQVARDSGLPALLSGGVRALADLEVARGV"
     gene            complement(608..1246)
                     /gene="hisH"
                     /locus_tag="VEL74_22500"
     CDS             complement(608..1246)
                     /gene="hisH"
                     /locus_tag="VEL74_22500"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HYG65370.1"
                     /translation="MSTRALIIDAGVGNLGNLVRALEHVGAATEITADPARVAAARCL
                     LLPGVGAFAPPREAVRGGVEGALREALDHGAWLLGICVGYQILFESGEEFGVTDGLGL
                     LTGRVTRLPDSVPVPHIGWNRLHDLTDHPLVAGLGEGSQGRYLYFVHSYAPEGVPDGL
                     CLARCTHGRAFAAVGGRGRVFGTQFHPERSGEAGLRLLANFLEMAHGPAAGH"
     gene            complement(1243..1854)
                     /gene="hisB"
                     /locus_tag="VEL74_22505"
     CDS             complement(1243..1854)
                     /gene="hisB"
                     /locus_tag="VEL74_22505"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HYG65371.1"
                     /translation="MTPRIGRVERTTRETRIRLALSLDGGPRRIQVPNGFFAHMLEAL
                     ATHGGLGLELEAEGDTHVDLHHTVEDVGIALGEALVEALGDRRGIVRFAHAYAPLDEA
                     LARAVVDLSGRGFFVYSAPSNVAAGWVTAEFPLTLVADFFQALADRGRLTLHLDILSA
                     RNGHHAAEAAFKAAALALRQAVSLREGTPETSEVPSTKGTLSA"
     gene            complement(1851..2945)
                     /locus_tag="VEL74_22510"
     CDS             complement(1851..2945)
                     /locus_tag="VEL74_22510"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HYG65372.1"
                     /translation="MSVKTEIDPVGFVRSELRRLGAYHLDLTPCRHKLDQNEVPWDLP
                     RRLKEEMTAGLLATDWARYPDFHADALRRDLGELHGWPWEGVLVGNGSNEWLGVTLTA
                     LVGPGLEVLGTEPSFSLYRSFVLKTGGVPRFLPPRPDLALPADDLEAEVERDPRRPLL
                     LCSPNNPTGSALTPDRIESLLEKLQAPLLLDNAYGEFSRHDYRPLLARHRHLILFRTF
                     SKAWSLAGMRLGYLVADPGLVTELIKVKLPYNLSHAAVHAGRAVLAAREEARRRVAIL
                     VARRPQWESMLAEEGLEVFPSEANFLLARCGSPEDARRIREGLETRSIRVREVGSYPG
                     LAGCLRVSVGNGASLRATRKALREIREEAR"
     gene            complement(2942..4240)
                     /gene="hisD"
                     /locus_tag="VEL74_22515"
     CDS             complement(2942..4240)
                     /gene="hisD"
                     /locus_tag="VEL74_22515"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HYG65373.1"
                     /translation="MTALQVLRSGTPEATEALDRLTRRAEEVLDPATLAGAADILARV
                     RREGDDGLLELARRLDGADAASVADLALRPFEGDLDGFVLPNGFAEALGRAIDAVGEF
                     HRPQVRSGYVMERDGVELEERATPLRRVGVYVPGGRASYPSTVVMTVVPAQVAGVREI
                     AVATPAGSYLSNAALRYTLARLGVQEVWGIGGAQAVAAFAYGTATIRRVDKIVGPGNA
                     WVTAAKKLVVGDVAIDGIAGPSEVVIVADSGSDPALVAADLLAQAEHDPLAAAVLITP
                     DEALARAVAGEIEEQLGELSTAEIARESLRRFGLALVVQDLEEALEAVERIAPEHLQL
                     VGPGAERLAWRVVAAGAIFMGADTPEAFGDYIAGPSHVLPTCGSARFASALGVEDFLR
                     RSHRVRFTAEAARRDAWAAAVLAEAEGLPAHQASAERRRG"
     gene            complement(4237..4851)
                     /gene="hisG"
                     /locus_tag="VEL74_22520"
     CDS             complement(4237..4851)
                     /gene="hisG"
                     /locus_tag="VEL74_22520"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HYG65374.1"
                     /translation="MALPKGRNLEVTLTAFRAAGIPLGLDPASRSLRTELPDAGIEVL
                     SLKDWDLPLYVGHGIADCGIVGSDVLEELDGDLLVPVRLRDGRSRLSLIGRTPEPPAT
                     GGQVRLATKYPNIARRVVATRPWGAEILKLWGSVELAPVLRLADVALDIVQSGRTLVE
                     NNLVEIETVAEVAACLVVNRASFQRHRARLNDWIDCLEAAGVVS"
     gene            complement(4857..5879)
                     /locus_tag="VEL74_22525"
     CDS             complement(4857..5879)
                     /locus_tag="VEL74_22525"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HYG65375.1"
                     /translation="MRVSATLPNGVAALLFDSARRRREAEGHMVGLLQGAGFSEVILP
                     ILDYLEPYDALLTTTSRNELYRFIDRDGEQLALRADFTPMLARLLAPRVASLPMPLRI
                     YYRGDVVRYEEERAGGQREFYQVGAELLGIPGAQAEDEVLRLFLEMLMVGGEGEMQIV
                     LGFAGALDRLLTQGAMEPSVLAVAVFRRERSTVRQACPKLLPVLESGLPDRPEDLGPE
                     AAHRLEGVLALRDELARRFPSIRLIVDLAEFARHSREPHLSTGSGERSYYDGIVFRAY
                     ASSAARPIGGGGRYDSLFRRLGAETPAVGFSLNLERLLEARSGSGLSVHPAARLSTQL
                     LGEVRP"
     gene            complement(6178..6780)
                     /locus_tag="VEL74_22530"
     CDS             complement(6178..6780)
                     /locus_tag="VEL74_22530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65376.1"
                     /translation="MMMRDSGGEVHGLLRQRWNQLLDAGETNPDELLSDLAEEPHSFI
                     FGHVALACLDLRRYDKEIQDIACTDVEGRVRAMAVRLQGVSDRLGQLSRLSQFYLWPL
                     QLLLDSEGLDPAAVKNSAENSARSAAEYAAWLCIKCVQSPVWIKGGGDAGSWGFNVKN
                     TQAIVTSLVEFWRYALASEEREARFEEVFSRLETPPSLRP"
     gene            complement(7780..9357)
                     /locus_tag="VEL74_22535"
     CDS             complement(7780..9357)
                     /locus_tag="VEL74_22535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65377.1"
                     /translation="MSQADFSQFEYVLTFLSSRMAPPGWLEEVDPQVKRRHGVAESRP
                     VQQEGQTKDEGVKRQLVVLLGAGCSRQYGLPSFLELLRYLWEDCLREAPEDDMGLEVL
                     RDKLDKFWQGQGPNYRTHMLKRYLRHGRVDGATCPGYVHLARLAKEGYVKAIINMNFD
                     TLLEDAIYHCGVPYRVATSFLVQDDKRIVIYKPHGTVGRVDSEDSIEGLLRAAQEVLD
                     AEKSLETEKDALLRIFNRALEHFQCDGEHLVQAANGKMEGSKTLARLCSLFLGLEEAA
                     PGEIRPELPVLREALESLIRAFRSPVKAKNDLILDIANSDLLSDSEEQRCAQELLTGH
                     DVVSIGYSGVDAKIAAALRAFTSNTDPKDKKLFVVNIAPPDPRLLSVMALRASQDLLI
                     AGRGAAFENFMGYLAKAVERLGMGQATQGERAEEDKKQSKKEKHSPAHMSAMTDAEQK
                     ALGHSLRLAQDIRLSMNVAERSRTSIEEHGYKIFNDCLELAQRAGICLNSAEKYLSIA
                     PLFCTTWFITEVIHGQR"
     gene            complement(9491..11314)
                     /locus_tag="VEL74_22540"
     CDS             complement(9491..11314)
                     /locus_tag="VEL74_22540"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HYG65378.1"
                     /translation="MSDPDHHRPILLDGNSLTLEGLARVARDPRVQVEITPEALARVG
                     ESWSQIERLADRYKQDFARLQHEDGVHPVLEYGVTTGFGEFKNIAVAPEDLERLQCKI
                     LLSHSVGLGETADELHPANYFDPEVVRAALVIRLNAFLKGHSGVRPEMVRIVQAMINR
                     GVVPLVPIRGSVGSSGDLCPLSHLFVVLLGESRYYLVQEPGDLSYQPRELRSGKQLGE
                     DLGLPVLPPSYKEGLALTNGATFSAALLALAVHDGERLAQVADVACALSLEAVCGCAR
                     AFDPRVHEARGHAGQIACAANIRALLRGSRLIEKAGAVQDVYSLRCAPAVHGSARDTL
                     AYARRVVEVEMNSATDNPLFFPSSEGSPFDYEFRDNWPEGYSGEDRISYSAGNFHGEP
                     VGVAADCLAIGLAELADISERRIQMLLDSDHSRNLPSNLVPHRGMNSGYMIAQYAAAG
                     VVSENKVLSHPASTDSIPTSANAEDHNAMATIACRKLRTILRNSQAVLAIELLVAAQA
                     VEWRVGMEIDPRAPKVKKPTGASNGKFLAEWDMAEAEAQAFATCVARERRPGIAAHLG
                     EGTRQAYLRVRDLAPPLLDDDRTLDVEVRAVRGAIEANTLL"
     gene            complement(11311..12753)
                     /gene="hisS"
                     /locus_tag="VEL74_22545"
     CDS             complement(11311..12753)
                     /gene="hisS"
                     /locus_tag="VEL74_22545"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008204238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HYG65379.1"
                     /translation="MSDKVEPRLSRGLRDLLSDQMLARQWMIDTIRGVYELYGFVPLG
                     TPAVEYLDVLLGSAAGAEAQESIFTVQNPEKEKLGLRFDLTVPLARVIAQYRDLPRPF
                     RRYQVSPVWRGDKPGPGRFREFTQFDLDSVGVESEVADTEILAGMCDTLGALEVGPYL
                     VRFSSRGILNVLLSYAGIPAERGADVFRVLDKLDKVGAQKVRLELTVGYVDESGDPIR
                     GLGLENVQVDKIERFLQIRADDRRAVVAELRDLLGGVAESDREIGIVERISNHLYSLG
                     YGDDRVSLDLSIARGLAYYTGPVFEAILLDAKQFGSVFGGGRYDGLVMRFLGERVPAT
                     GASIGVDRLLAALMHLGRAGQQKCTAQVLVTSMDSALTDDYIAMTWELRRAGIPAELY
                     LGTAKGVGKQLKYGDAWGVPIALLYGSDEKARGVVTLKDMDAGRQRAQALADRREWLE
                     ERPGQREVPRGELVATVRQMLADVARGQGR"
     gene            complement(12794..12868)
                     /locus_tag="VEL74_22550"
     tRNA            complement(12794..12868)
                     /locus_tag="VEL74_22550"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12834..12836),aa:His,seq:gtg)
     gene            complement(12944..13951)
                     /gene="egtD"
                     /locus_tag="VEL74_22555"
     CDS             complement(12944..13951)
                     /gene="egtD"
                     /locus_tag="VEL74_22555"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HYG65380.1"
                     /translation="MTRALEKDRLSIHEVAAGAAGSTFADDVHRGLTASPKTLSPKHL
                     YDAMGSKLFEAICELPEYYLMRAEAEILHAHAGDVAAAFDGRVRLVELGSGDGQKTRL
                     LISALLARQGGLEYLPIDISPSAIEQGPGRLLSGYPGLRVSAFVGEFLPGLQALGRER
                     PREPGTRTLALLLGSTLGNLEPDERLVLLRGIRDLLLPGDGFLLGLDQRKPDEVLLPA
                     YDDGLGVTAAFNLNLLVRINRELGGRFDLRSFRHVARYDRGLGRIEMHLESLRSQSVP
                     IQALGAVIGFEAGETIHTESSYKFELGQVARLAAETGFEVRHTWLDGGQRFGSFLLMA
                     A"
     gene            complement(13948..15462)
                     /locus_tag="VEL74_22560"
     CDS             complement(13948..15462)
                     /locus_tag="VEL74_22560"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HYG65381.1"
                     /translation="MERTGSIGNAGNASLFATLTAPPDEDLEAQLGGFDGVEWLEVRA
                     DLAGDLDPAPLRARFPGRLLYTLRSRAEGGAHEGSAERRKRRLIDAAARYDRVDLEAS
                     RDLSPDLLKAIPPDQRLLSWHGPAPGLPALKTLCERMTAVEAALYKLVPTASQPGEDL
                     QPLLLLHALERPDVAAFASGEAGVWTRMVAPRLGAPVVYGAAGEVPGAPGQPSIRRLR
                     EDFGLPGLPSIRVLFGLVGNPVSHSLSPRIHNAAYRELGIPAAYLPFHTDSFGDLWLE
                     LVEGGALESLGLPIRGLSVTAPYKEAALAVAGAESPLAEKIGAANTLVWNQGVWEAES
                     TDPDGVVLPLHERGVRVEGLRAAVVGTGGGGRSAAVGLVRAGADVTLFNRGAARGEKA
                     ASDLGLPFLPLGELDPAAFGLLVNATALGRSVSDPLPFLVDDLRLGTVVIDLVYLKDG
                     PTPLLVAAAERGAVVVDGREVLLDQARGQFRMMTGQEMPVGLARRMLGLEGEGR"
BASE COUNT         2625 a         5598 c         5349 g         2429 t
ORIGIN      
        1 caccccgcgc gccacctcca gatccgccag cgcccgcacg ccaccggaga gcagggcggg
       61 cagccccgaa tcccgggcca cctgacgggc cagctcgacg ttggggccgg agagcatgcc
      121 gtcccgctct acgtcggtca ccaggacggc cgggcagggg agaccgcgca gcccggcgca
      181 gaggtccgcg agggtccgcc gcgccgactc ggtccagccc gccacccgga cttccccctt
      241 ctcgatgtcc agcgccggca cgagcctccc gggcaactcg tgggccagcg cgccgaaggc
      301 ttcggggtcc cgcgccacca gggaaccgat gaccacgcgg tcgcagccag cccctagcgc
      361 ccactcgacc gcctccctcc cccgcaggcc gccgccgagt tggatcgcca ccgcttttga
      421 ggccgccatg taggcaataa ggtcccgctg cggcgcctcg ccgagggccg cgtccaggtc
      481 caccacgtgg acccgcgcca cgccggcccg ggcgtaggac tccagggccg cgaccgggtc
      541 gtcgccgtag acggaggcgc gggcgtcgtc cccctggcgg agacggacgg cgcggccgtg
      601 ccgcaggtca atggccggca gcaggtccat gggccatctc caggaaattg gcgagcaggc
      661 gcaggccggc ctcgccgctg cgctccgggt ggaactgggt gccgaagacg cgtccccgcc
      721 cccccaccgc ggcgaaggct cgaccgtggg tgcagcgggc caggcagagg ccgtccggca
      781 ccccttccgg cgcgtagctg tgaacgaagt agaggtagcg gccctgactc ccctctccca
      841 ggccggcgac cagagggtga tcggtgaggt cgtgcaggcg gttccagccg atgtggggca
      901 ccggcacgct gtccggcagc cgggtcaccc ggccggtgag cagccccagg ccgtcggtga
      961 cgccgaactc ctcgcccgac tcgaagagga tctgatagcc gacgcagatg cccagcagcc
     1021 aggcaccgtg gtcgagcgcc tcccgcagcg ccccttcgac gccgccgcgc acggcctccc
     1081 tgggtggcgc gaaggccccg accccaggga gcagcaggca gcgcgccgcc gccacccgcg
     1141 ccgggtcggc cgtgatctcc gtcgccgccc cgacgtgctc cagggcgcgc accaggttgc
     1201 ccaggttgcc gacgccggcg tcgatgatca gcgcccgcgt gctcatgcgg agagggtccc
     1261 cttggtgctg ggcacttcgc tggtctccgg ggtcccttcc cggagcgaga ccgcctgccg
     1321 gagcgccagg gccgcggcct tgaaggcggc ctcggcggcg tggtggccgt tgcgggcgga
     1381 caggatgtcc aggtgcaggg tgagccggcc gcggtcggcc agcgcctgaa agaagtcggc
     1441 caccaaggtg agcgggaact cggccgtcac ccagccggcg gccacgttcg acggtgcgct
     1501 gtagacgaag aagccgcggc cggagaggtc caccaccgcg cgggcgagcg cctcgtcgag
     1561 cggcgcgtag gcatgggcaa agcggacgat gccccgccga tcccccagcg cctcgaccag
     1621 cgcctcgcct agggcgatcc ccacgtcctc gacggtgtgg tggaggtcca cgtgggtatc
     1681 tccttcggct tccagctcca gcccgaggcc gccatgggtg gccagcgctt ccagcatgtg
     1741 ggcgaaaaag ccgttcggca cctggatgcg ccggggcccg ccgtccagag acagggcgag
     1801 gcggatgcgc gtctcccgtg tcgtcctctc gactctaccg atccgtgggg tcatcgcgcc
     1861 tcctcccgaa tctcgcgcaa ggccttgcgg gtcgctcgca acgatgcgcc gttccccacc
     1921 gacacccgca ggcagccggc gagccccgga taggagccca cctcccgcac tcggatgctc
     1981 cgtgtctcca gcccctcgcg gatccgccgc gcatcctccg gagatccgca gcgcgccagc
     2041 aggaagttgg cctccgaagg gaagacctcc agcccctcct cggcgagcat gctctcccac
     2101 tgtggccgcc gggcaacgag gatggcgacg cggcggcgcg cctcctcgcg ggcggcgagg
     2161 acggcccggc cggcgtggac cgcggcatgg ctcaggttat aaggaagctt gaccttgatc
     2221 agctccgtca ccagccccgg gtcggccacc aggtacccga ggcgcatgcc ggccagtgac
     2281 caagccttgg agaaggttct gaagaggatt agatgacggt ggcgcgccag cagcggccgg
     2341 tagtcgtggc ggctgaactc gccgtaggcg ttgtcgagga ggagcggcgc ctggagcttt
     2401 tcgagtagcg actcgatccg gtccggcgtc agggcggagc cggtggggtt gttgggcgag
     2461 cagaggagca gggggcggcg cgggtcgcgc tccacctcgg cctccagatc gtccgcgggg
     2521 agcgccaagt ccggccgcgg cggcaggaag cgcgggacgc cgccggtctt gaggacgaac
     2581 gagcggtaga ggctgaagct gggttcggtg cccagcacct ccaggcctgg ccccaccagc
     2641 gcggtgaggg tcacccccag ccactcgttg gagccattgc ccaccagcac cccttcccac
     2701 ggccagccgt gcagctcccc gaggtcgcgg cgcagggcgt cggcgtgaaa gtccgggtag
     2761 cgggcccagt ccgtagcgag gagccccgcc gtcatctctt ccttgagccg gcgcggcagg
     2821 tcccagggga cctcgttctg gtccagcttg tggcggcacg gagtcaggtc caggtgatag
     2881 gcgccgagcc gccgcagctc ggaccggacg aatcccaccg gatcgatctc cgtcttcacg
     2941 ctcatccccg cctcctctcc gctgacgcct gatgggccgg caacccttcg gcctcggcga
     3001 ggaccgccgc cgcccatgcg tcgcgccgtg ccgcctccgc cgtgaagcgc acccggtggc
     3061 tccgccgcag gaagtcctcg acgcccaggg ccgaggcgaa gcgggcgctg ccgcaggtgg
     3121 gcagcacgtg gctcgggccg gcgatgtagt cgccgaaggc ctcgggcgtg tccgccccca
     3181 tgaagatcgc ccccgccgcc accacccgcc aagccagcct ctcagccccc ggcccgacga
     3241 gttgcaggtg ctccggcgcg atccgctcca cggcctccag cgcctcctcg aggtcctgca
     3301 ccaccaaggc caggccgaac cgccgcagcg actctcgagc gatctccgcg gtcgacagct
     3361 ctccgagctg ctcctcgatc tccccggcca cggcccgcgc cagcgcctcg tccggggtga
     3421 tgagcaccgc cgccgccagc gggtcgtgct cggcctgggc cagcaggtcg gccgccacca
     3481 gggccgggtc ggacccggag tcggcgacga tcaccacctc gctggggccg gcgatgccgt
     3541 cgatggccac gtcccccacc accagcttct tggccgcggt gacccaggcg ttgcccgggc
     3601 cgacgatctt gtccacccgg cggatggtgg cggtgccata ggcgaaggcc gccacggcct
     3661 gagcgccgcc gatcccccag acctcctgca cgcccagccg cgccagggtg tagcgtagcg
     3721 ccgcgttgga taggtacgag ccggccggcg tggcgacggc gatctcgcgg acgccggcca
     3781 cctgggcggg caccacggtc atcaccacgg tcgaggggta gctcgcccgg ccgcccggca
     3841 catagacgcc gacccggcgc agcggcgtcg ctcgctcctc caactccacc ccatcgcgct
     3901 ccattacgta gccgctccgc acctgcggcc ggtggaactc gccgaccgcg tcgatggccc
     3961 ggcccagcgc ctcggcgaag ccgttcggca ggacgaaccc gtccagatcc ccttcgaagg
     4021 ggcggagggc gagatccgct accgaagcgg cgtccgcccc gtccagccgg cgcgccagct
     4081 cgagcagccc gtcgtccccc tcacggcgga cgcgggcgag aatgtccgcg gcgccggcga
     4141 gcgtcgccgg atcgagcact tcctcagcgc ggcgggtgag gcggtccagg gcctcggtgg
     4201 cctcgggcgt gccggagcgc agcacctgaa gggcggtcac gacaccactc ccgccgcctc
     4261 gaggcagtcg atccagtcgt tgaggcgggc gcgatggcgc tggaaggacg cgcggttgac
     4321 caccaggcag gcggccacct cggccacggt ctcgatctcc accagattgt tctccaccag
     4381 agtgcggccg ctctggacga tgtcgagcgc cacgtcggcc aggcgcagca ccggcgccag
     4441 ctcgaccgat ccccagagct tgaggatctc ggcgccccaa gggcgggtcg ccaccacccg
     4501 gcgcgcgatg ttcggatact tggtcgccag gcgcacctgc ccacccgtgg cgggcggctc
     4561 cggcgtacgg ccgataagcg acagccgcga gcgcccgtcg cgcaaccgca ccggtaccag
     4621 caggtcgccg tccaactcct ccagcacgtc gctgcccacg atgccgcagt cggcgatccc
     4681 gtgcccgacg tagagcggca ggtcccaatc tttcaaggac agcacctcga tcccggcgtc
     4741 cggcagctcg gtccgcaggc tgcggctcgc cggatccagg ccgagaggga tgccggcggc
     4801 gcggaaggca gtcaaggtca cctccaggtt cctccccttg ggcagggcca tgcggatcac
     4861 ggccggacct cccccagaag ctgggtggag agacgcgcgg cggggtggac ggagagaccg
     4921 cttcccgatc gggcctccag caagcgctcc agattcaggg agaacccgac agcgggcgtc
     4981 tcggccccga gccgccggaa gaggctgtcg taccgtccgc ccccaccgat cgggcgagcc
     5041 gcgctgctgg cataggcgcg gaagacgatc ccatcataat aagaacgctc gccagacccg
     5101 gtggacaggt ggggctccct gctgtgacgc gcgaactcgg ccagatcgac gatcagccgg
     5161 atcgagggaa agcggcgcgc cagctcgtct cggagggcga gcaccccctc caggcgatgg
     5221 gccgcttccg gccccaggtc ctcaggccgg tcgggcagcc ccgattccag gacgggcagc
     5281 agcttggggc aggcctgccg taccgtgctc cgctcccgcc ggaagactgc cacggccagc
     5341 accgatggct ccatcgcacc ctgggtgagg agccggtcga gggcaccggc gaacccgagc
     5401 acgatctgca tctcgccctc tcctccgacc atcagcatct ccaggaagag gcggagcacc
     5461 tcatcctccg cctgcgcacc aggaattccc agcagctcgg cgccgacctg atagaactct
     5521 ctctggccgc cggcccgctc ttcttcataa cgcacgacgt cgccacgata gtagatgcgc
     5581 aacggcatag ggagcgacgc aacgcgtgga gccagcagtc gggccagcat cggggtgaag
     5641 tcggctcgca gggcgagctg ctctccatcg cggtcgatga agcggtagag ctcgttgcgg
     5701 ctcgtggtcg tcagcagcgc gtcgtagggc tccaggtagt cgaggatggg caggatgacc
     5761 tcggaaaagc ccgccccttg gaggagaccg accatgtgtc cctccgcttc gcggcggcgc
     5821 cgcgcgctgt caaagagcag ggccgcgaca ccgttgggga gcgtggcgga gactctcatg
     5881 ccaacctcct accccggcgg gtcgtcgcgg agagctctct cccgttcgct atctccaaaa
     5941 cgaaaaacct ccttccgggc ccggaaggag gtggcatggt cagcggcagc agcagctctc
     6001 gtcccgggtc cgtgtgccgg gatctctgcc ccggctagct cacgacgtgg tgatggacat
     6061 ggaccgacca ccagccctgg aggggctcgg cggaagctgc ggaaaggcgg gcggtctcgt
     6121 agggcatcat cacagaccca ttacgaagag cagcttacgc agccggctgg tggattgtca
     6181 aggccggagc gaaggcggcg tctcaaggcg gctgaagacc tcttcaaatc ttgcctcccg
     6241 ctcttcactc gccagggcat agcgccagaa ctctactagg gaggtcacga tcgcctgcgt
     6301 gttcttcaca ttgaaccccc acgaccctgc gtcgcctccc cccttgatcc agaccggtga
     6361 ttggacacac ttgatacaga gccacgctgc gtactcggca gcactcctgg cggagttctc
     6421 ggcagagttc ttgacggcgg caggatcgag gccctcactg tccaggagca gttgcagcgg
     6481 ccagagatag aactgactga gccgggagag ttgcccgagc cggtctgaca caccttgcag
     6541 gcggacggcc atggcacgta cccgtccttc cacatcggta caggcgatgt cttggatctc
     6601 tttgtcgtag cgcctcaggt ccagacatgc caaggcgacg tgaccgaaga taaaggagtg
     6661 gggttcttcg gccaggtcgc tgagcaactc gtccgggttc gtttcgccag catccagaag
     6721 ttgattccag cgctggcgca ggaggccgtg aacttctcct ccggaatccc gcatcatcat
     6781 gccagttacg agcgaagaag cgccaggccg ggtatattat atcttcaaag ctcttacaga
     6841 agaggttccg catagtgctc aagtcgagcc tgccgcgatg cttccgccaa aggtagtgcc
     6901 acaggagccc gtgctgtgac acgaactgct tctggttcct gacctccaac gtccggtgca
     6961 gagcccggtc gagcacggaa ggaaaagtag acccgctctc cggcgatctc tcgtcgagct
     7021 tcactcggac ctcttgctgg ctggcgctca tcgtatagat cgaaatcagg tcaagcaatg
     7081 ccgaagcctc tcctgggtaa ccaggccgga cctccaagag gcttctctcg agcatttcgg
     7141 tgagagcgcg acacaggaga ggctcgaact ggcgcttcaa ggagtcgcct tccgtggaca
     7201 gcagactcga ctcccagcga gggtctagcc tgatccccca accaccttcc ttgcgcgctt
     7261 cctcgtcgaa ccggcggatg tgataggcgg ccatggtcaa cagccagagt atggttgctg
     7321 gccgcttact atccttctcg tcgactacga tagaccgcat cctgtagttg aaatcgatcg
     7381 gcctctcggt ctgacgcaag tagaggtcga gcactggatc tgcaatagac cacgtgctct
     7441 tcctgggcga gggccccatg aagtccggtg aaggaacgaa tgggtgcccc tccgagaggt
     7501 cctccgcaag agcgaacaaa gagagcagca gatcaaagcg taccttgacc ttaacgccgt
     7561 cgatcgacag ctcggttttc tttggtgcct cgacctccac caggccggag tgtccttcac
     7621 acaaggcaca gagcacttct tcaaaggcgt ccagctcttt gtagatcgac ggcttgatga
     7681 gcttcagact cgactcgggg tcgtccccga accgttcccg cagaagctcc cgagtcagca
     7741 ttccatgctt ctccaggagt gtccatcctg ggtagtcgct cacctttgcc cgtgtataac
     7801 ctcggtaata aaccaggtcg tgcagaaaag cggtgcaatg gagaggtact tctccgccga
     7861 gttcagacag attcccgcac gctgagcaag ctccaggcag tcgttgaata ttttatagcc
     7921 gtgctcctct atgctggtcc ggctacgctc ggctacgttc atggataggc gaatgtcctg
     7981 ggctagtcgg agactgtgcc ccagggcctt ctgctcggcg tctgtcatgg cgctcatgtg
     8041 tgcaggagaa tgcttctcct tcttagactg cttcttatcc tcctctgctc tctctccttg
     8101 cgtcgcctga cccatcccga gccgctcgac agccttggcg agatatccca tgaaattttc
     8161 gaacgctgca ccgcgtcccg caatcaggag atcttgggag gcccgcagag ccatgacgct
     8221 gagcaaccgg ggatcgggag gcgcgatgtt cacaacgaag agtttcttgt ctttcggatc
     8281 ggtgttcgat gtaaaggccc gcagagcggc agcgatcttc gcgtccacgc ccgaatagcc
     8341 gatcgacacg acgtcgtggc cagtcagaag ctcctgcgca caccgctgct cttccgagtc
     8401 cgagagcaga tcactgttgg cgatatcgag aatgagatca ttctttgcct tcaccgggga
     8461 tcgaaaggct cgaatcaaag actccagcgc ttcacgcaat acagggagct ccggcctaat
     8521 ctctcctggc gccgcctcct ccagccccaa gaagagagag caaagacgag ccagcgtttt
     8581 ggaaccttcc atttttccgt tggcggcctg cactaggtgc tcaccgtcgc actgaaaatg
     8641 ctccagggcc cggttgaaaa ttcggagcag agcgtccttc tcggtctcca gcgatttctc
     8701 ggcatcgagg acctcctggg cggctcgcag gagaccctcg atggagtctt cggaatccac
     8761 tcggccgact gtgccgtgcg gtttatagat gactatcctc ttgtcgtcct gcacgagaaa
     8821 gctggtggct accctgtagg gcacgccgca gtggtatatg gcgtcttcga gcagggtgtc
     8881 gaaattcatg ttgatgattg ccttaacata cccctccttc gccaagcggg cgagatggac
     8941 gtatcctggg caggtagctc cgtccacccg gccgtgcctg agatagcgct tcaacatatg
     9001 cgtgcggtag ttggggccct gcccttgcca gaatttgtcg agtttgtccc gcagcacttc
     9061 gaggcccata tcgtcctcgg gcgcctcgcg cagacagtcc tcccagagat accggagcaa
     9121 ctccagaaag ctgggtaggc cgtactgccg cgagcacccg gcccccagga gtacgacgag
     9181 ttgccgcttg actccctcgt ccttggtttg gccttcctgc tggacaggtc ggctctcggc
     9241 aacgccatgt ctccgcttca cctgcgggtc cacttcctca agccagccgg gcggggccat
     9301 ccggctggac aggaacgtca ggacgtattc gaactgggaa aaatccgcct ggctcatctc
     9361 ctccccctct cttggacaga gcatcacgat ggcggctccg gcagcagcgg agcacacctc
     9421 ggcctttagg atttagacac tgacttactg tagatgttga gcggtgtcca ggcgaggccc
     9481 ggaggagggc tcagaggaga gtgttggctt cgatggcccc gcggaccgcc cgcacctcca
     9541 catccagggt gcggtcgtcg tccaacaggg gtggggcgag gtcgcgtacg cggaggtagg
     9601 cttggcgggt cccctcgccg agatgggcgg cgatcccggg ccgccgctca cgggccacgc
     9661 aagtagcaaa ggcctgcgcc tcggcttcgg ccatgtccca ctcggccagg aatttgccgt
     9721 tcgaggcgcc ggtcggcttc ttgaccttgg gcgcgcgcgg atcgatctcc atgccgaccc
     9781 gccactcgac ggcctgcgcc gccaccagca gctcgatcgc cagcaccgcc tggctgttgc
     9841 gcaggatcgt gcgcagcttg cggcaggcga tggtcgccat ggcgttgtgg tcctccgcgt
     9901 tcgccgaggt ggggatggaa tcggtcgagg cgggatggct gaggaccttg ttctccgaga
     9961 ccacgcccgc agcggcgtac tgggcgatca tgtatcccga gttcatcccc cggtgcggca
    10021 ccaggttcga gggcaggttg cggctgtggt cggaatcgag cagcatctgg atgcgccgct
    10081 cggagatgtc cgccagctcg gccaagccga tggccaggca gtccgccgcc acccctaccg
    10141 gctcgccgtg gaagttgccg gccgaatagg agatccggtc ctcgccggag tagccctcgg
    10201 gccagttgtc ccggaactca tagtcgaagg gggagccctc gctgctcgga aagaacagcg
    10261 ggttgtcggt cgccgagttc atctcgacct cgaccacccg gcgcgcgtag gcgagggtgt
    10321 cgcgcgccga gccatggacg gccggggcac agcgcaggga gtagacgtcc tggaccgcgc
    10381 cggccttctc gatcagccgg ctcccccgga gcagggcgcg gatgttggcc gcgcaggcga
    10441 tctgcccggc gtggccgcgc gcctcgtgga cgcggggatc gaaggcccgg gcacagccgc
    10501 acaccgcttc gagggagagg gcacaggcga cgtcggccac ctgcgccagg cgctctccgt
    10561 cgtggacggc gagcgccagc agcgccgccg agaaggtggc gccgttggtc agcgccagcc
    10621 cctccttgta ggacggcggc aaaaccggca gccccaggtc ctcccccagt tgcttcccgc
    10681 tccgcagctc gcgcggctgg taggagaggt cgcccggctc ctggacgagg tagtagcggc
    10741 tctcgccgag cagcaccacg aacaggtggg acagcgggca gagatcgcca ctcgacccca
    10801 ccgagccccg gatcgggacc agcggcacca ctccgcggtt gatcatcgcc tggacgatcc
    10861 gcaccatctc cgggcgtact cccgagtgcc ctttcaggaa ggcgttcagg cggatcacca
    10921 gcgccgcgcg caccacctcg ggatcgaagt agttggccgg gtgcagctcg tcggcggtct
    10981 cacccagccc gaccgagtgg ctgagcagga tcttgcactg gagccgctcg agatcctccg
    11041 gcgccacggc gatgttcttg aactcaccaa agccggtcgt gacgccgtac tcgaggaccg
    11101 gatgcacccc atcctcgtgc tggagcctcg cgaagtcctg cttatacctg tcagcgagcc
    11161 gttcgatctg gctccagctc tcgccgaccc gggcgagggc ctccggcgtg atctccacct
    11221 ggacccgcgg gtcacgggcg acccgtgcca gcccttcgag ggtcagggaa ttgccgtcga
    11281 ggaggatcgg gcgatgatgg tcggggtcgc tcaccggccc tgccctctcg cgacatcggc
    11341 cagcatctgg cggacggtgg cgaccagctc gccgcgcggc acctcgcgct ggcccggccg
    11401 ctcctcgagc cactcgcggc ggtccgcgag cgcctgggca cgctgccgcc ccgcgtccat
    11461 gtccttgagg gtgacgacgc cacgggcttt ctcgtcggag ccgtagagga gggcgatggg
    11521 cacgccccag gcgtccccat acttgagctg cttgccgacc cccttggccg tgccgaggta
    11581 gagctcggcc gggatgcccg cccgccgcag ctcccaggtc atggcgatgt agtcgtccgt
    11641 gagcgccgag tccatcgagg tcaccaggac ctgcgccgtg cacttctgtt ggccggcgcg
    11701 gccgaggtgc atcagcgcgg cgaggaggcg gtcgacgccg atcgaggccc cggtagccgg
    11761 caccctctcg ccgaggaaac gcatcaccag gccgtcgtag cggccgccgc cgaacaccga
    11821 gccgaactgc ttcgcgtcga gcaggatggc ctcgaaaacc gggccggtgt agtacgcgag
    11881 gccgcgggcg atcgagaggt ccagggagac ccggtcgtcg ccgtagccca gggaatagag
    11941 gtgattcgag atccgctcca cgatgccgat ctcgcggtcg ctctccgcca ccccgcccag
    12001 gaggtcgcgc agctccgcga ccacggcgcg ccggtcgtcc gcgcggatct ggaggaagcg
    12061 ctcgatcttg tcgacctgga cattctccag gccgaggccg cggatgggat ccccggactc
    12121 gtccacatag cccacggtca gctccagcct gaccttctgc gctcccacct tgtccagctt
    12181 gtcgaggaca cggaacacgt cggccccgcg ctcggccggg atcccggcat agctgagcag
    12241 gacgttcagg atgccgcggc tggagaagcg gaccaggtac ggaccgacct ccagggcgcc
    12301 cagcgtgtcg cacatgccgg cgaggatctc ggtgtcggcc acctcggact cgaccccgac
    12361 cgagtcgagg tcgaactggg tgaactcgcg gaagcgtccc ggccccggct tgtccccccg
    12421 ccagaccggc gacacctggt agcggcggaa cgggcggggc aggtcgcgat actgggcgat
    12481 gacccgggcg agagggacgg tcaggtcgaa gcggagcccc agcttctcct tctcggggtt
    12541 ctggacggtg aagatcgact cctgcgcctc ggccccggcg gcggagccga gcagcacgtc
    12601 caggtactcg accgccggcg tcccgagcgg gacgaagcca tacagctcgt acactccgcg
    12661 gatcgtgtcg atcatccact gccgggccag catctggtcg gacagcaggt cacgcaggcc
    12721 acgggagaga cggggctcca ccttgtcaga catgagacac gatcctcgaa aagggctggt
    12781 tcagagaaca aactggcacc ggcgatggga tttgaaccca tgacctcctg atccacagtc
    12841 aggcgctcta cccctgagct acaccggcgc atcggggaaa cggaatccta tgcgggcggg
    12901 ccggggcttg tcaaaacccc ccctcacaga gcgtgggcac ggctcaggcc gccatgagaa
    12961 ggaaggagcc gaagcgctgc ccgccgtcca gccaggtgtg ccgcacctcg aacccggttt
    13021 cagccgccag gcgcgccacc tgcccgagct cgaacttgta cgagctctcg gtatgaatgg
    13081 tctcccccgc ctcaaagcct atcaccgcgc cgagagcctg gatcggaacg ctctgcgacc
    13141 gcaggctctc cagatgcatc tcgatccgcc ccaggccgcg gtcgtagcgc gccacgtgac
    13201 ggaaggagcg gaggtcgaac cggcccccca gctcgcggtt gatccggacc aggaggttga
    13261 ggttgaacgc cgcggtgact cccaatccgt catcgtaggc gggcaggagg acctcgtccg
    13321 gcttccgctg gtcgagaccg agaaggaagc cgtctccggg aaggaggagg tcacggatcc
    13381 cgcgcagcag caccaggcgc tcgtccggct cgaggttgcc cagggtggag ccgaggagga
    13441 gggccagagt ccgggtgccc ggctccctcg gccgctcccg gcccagcgcc tgcaatccgg
    13501 gcaggaactc accgacgaag gcggagaccc gcagcccggg atagccggag aggaggcggc
    13561 cggggccttg ctcgatcgcc gagggcgaga tgtcgatcgg caggtattcg agcccgccct
    13621 ggcgcgcgag cagggcggag atcagcaggc gggtcttctg gccgtcgccg ctccccagct
    13681 ccacgagacg gacgcgcccg tcgaaggccg ccgccacatc cccggcgtgg gcgtggagga
    13741 tctcggcctc cgcacgcatc aggtagtact ccggcagctc gcagatcgcc tcgaagagct
    13801 tcgagcccat agcgtcgtac agatgcttcg gcgacagggt cttgggcgac gccgtcaacc
    13861 cacggtgcac gtcgtccgcg aaggtcgagc cggcggcgcc ggcggcgacc tcgtggatgg
    13921 aaagcctgtc cttctcgagc gctcttgtca tcgcccctcc ccctccagcc cgagcatccg
    13981 ccgggcgagg ccgaccggca tctcctggcc ggtcatcatc cggaactgcc ccctggcctg
    14041 atcgaggagg acctcgcggc cgtcaaccac caccgcgccg cgctccgccg ccgccacgag
    14101 cagcggcgtc gggccgtcct tcaaatagac gaggtcgatc accaccgtcc ccaggcgcag
    14161 gtcgtccacc aggaacggca gcgggtcgga gacggagcgg ccgagggcgg tggcgttgac
    14221 cagcaggccg aaggcggcgg ggtcgagctc gcccaacggg aggaacggca gccccaggtc
    14281 actggccgcc ttctcgccgc gcgccgcgcc gcggttgaac agggtgacgt cggcgccggc
    14341 gcgcaccagc cccaccgcag ccgagcgccc gccgccgccc gtgcccacca cggcggcgcg
    14401 cagcccctcg acgcggacgc cgcgctcgtg cagcggcagc accacgccgt ccgggtcggt
    14461 ggactcggcc tcccagaccc cctggttcca caccagcgtg ttggcggcgc cgatcttctc
    14521 ggccagcggg ctctcggccc cggccaccgc cagcgccgct tccttataag gagcggtcac
    14581 cgacagcccg cggatcggca gccccagcga ctccagcgcg cccccctcca ccagctcgag
    14641 ccagaggtcg ccgaaggagt cggtatggaa cggcaggtag gccgcgggga tccccagctc
    14701 ccggtaggcg gcgttgtgga tgcgcggcga cagcgagtgg ctcacgggat tgcctacgag
    14761 gccgaagagg actcggatgg atggcagccc ggggaggccg aagtcctcgc gcagccggcg
    14821 gatagacggc tgccccggcg cccccggcac ctcgccggcc gcgccgtaga ccaccggtgc
    14881 tccgagccgc ggcgccacca tgcgggtcca caccccggcc tcgccggagg cgaaagcggc
    14941 gacgtcgggc cgctccaggg cgtggagcag caggaggggc tggaggtcct cgccgggctg
    15001 ggaggcggtg ggcaccagct tgtagagcgc cgcctcgacc gcggtcatgc gctcgcagag
    15061 ggtcttgagc gccggcagcc ccggcgccgg tccgtgccaa gagaggagcc gctggtccgg
    15121 cgggatggcc ttgaggaggt cgggagagag gtcgcgggac gcttcgaggt cgaccctgtc
    15181 atagcgggcc gcggcgtcga tgagccgccg cttgcggcgc tcggccgaac cctcgtgggc
    15241 gccgccctcg gcgcggctgc gcagggtata gaggaggcgt cccggaaacc gcgcccgcag
    15301 cggcgcgggg tcgagatcgc cggcgaggtc ggcgcggacc tccagccact cgacgccgtc
    15361 gaagccgccg agctgggcct ccagatcctc gtcgggcgga gccgtgaggg tggcgaacag
    15421 cgaagcgtta cccgcgttgc cgatgctgcc ggttctctcc aaaatcgaaa acctccttcg
    15481 agacccggaa ggaggcgacg gtggggcggt ctctcgctcg tcccgggctc gcgatgccgg
    15541 gacgtacccg gcctcaggtc acgccgtgat gcgcatggcc ccaccatcca gccgctccga
    15601 cgaggagggg gttgctgacc gtggggcaag gcgtctgcag gcgcatcatc gaatcccgtt
    15661 ttacacggat gggcagctta tgcagggggg aacggggatg tcaagcgagg cccgcgagct
    15721 ccgcttcggg gcgaagaaga agggttcctc tccgaattca tcgacgttgt cgcggttgtc
    15781 gcgctcgtgt gagcgccgca ccgcaccgta ttatttaata cctgctacta ttggaaatgg
    15841 cagcgcctga ggggcccccg gatgaaagca aactcgtgac gacttgcccg agaccgtctt
    15901 cggcggccgc tactacgacg agggcctctc catcgacgtc gacgcccagg gccggatcta
    15961 tgtcgtcggc ttcacccact cgtgggattt cgcggacccg g
//
LOCUS       DASPJI010000800         5155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_729086, whole genome shotgun sequence.
ACCESSION   DASPJI010000800 DASPJI010000000
VERSION     DASPJI010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729086"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /locus_tag="VEL74_22565"
     CDS             <1..1581
                     /locus_tag="VEL74_22565"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYG65382.1"
                     /translation="PAPEAAEPEPGVSSSSYALLDCPEGVVAEVEFPLTVGISPLPMV
                     PGDEPLDLPPTPGYVLVVHVIAEGFRLRAGEPERHELAVTHENPYPTVVLHLTPDPQD
                     TRVRLRRIRANYSVGGQAIGFADRPLVVVESAELLDSQIPRPVPRPGTIHVPTAGTAP
                     DLTLTIRLGDSVGDLLWTLESPHAIELPDRRLQSDIGKEPDRFARQIVDLVNGREGTT
                     GLYTFLVGIGREVTQEVPVKVWKALRAAAAKRGGPPDLLILSQEPYIPWELAVMPEPL
                     LDPQAPPFLAAQANVGRWIIRKDLDEEDGGSLDRPRLPPPTELAIKGLAVVSGVYSSG
                     KWQRLKEAEQEAAELAQLYPAASVNADFETVTGCLKGIPEADMLHFAVHGVYNPESVE
                     HGLMLVDKRVLHPFDVKGIDLTRAPFVFLNACQVGSGEQVLGDYAGLAEAFLFAGASG
                     VIAPLWSVKDTVARQVALDFYREIAAGRTPADIVRAARARFKDAAGPLSATYLAYQFF
                     GHPQLKLTGLRPREEGQP"
     gene            1578..3074
                     /locus_tag="VEL74_22570"
     CDS             1578..3074
                     /locus_tag="VEL74_22570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65383.1"
                     /translation="MSERLDPSGSPVRVGGITLRTPGLEGVVSVMPPTSAETRAAAFD
                     SAALEEALREEGVATDETLEITDTNEVEAGGGEGTRTAQGEPAIELEVWEPGEEWGQI
                     VLYTDEAGVTTWAFSLPGEAAAPGERVRGGAGGRTYVLRRHVPPAPQGEATRGVIGAA
                     GKKIIRVLSFKLLDPVFGRVGEHFVAKWEQEKRPYRVRTFPPEGFRNASAKLVDLPDW
                     PALATSDGKRALLFVHGTFSRAHAAFGGLPPEFMEALHRRYEGRVFAFDHPTASEDPR
                     QNVEWLLQEIPDRTRLDLDIICHSRGGLVSRVLAEKQADLALGSRQVRVHNVVFVAAP
                     NAGTILVNEEHMGDFVDSYTNLLNFFPDNGVTEGLEWIITVAKHLACSTLKGLEGLQS
                     MRPGGGFLTGINGATSPDKRYYAVASDFEPADLGLSSFKDGVMDRIFGHPNDLVVPTA
                     GVWDHNGGSLFPIAEKLVLPDKGIHHSGYFSCPEVQRQIARWLEVETG"
     gene            3234..4856
                     /locus_tag="VEL74_22575"
     CDS             3234..4856
                     /locus_tag="VEL74_22575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG65384.1"
                     /translation="MRAVCWAVCLSVTLAGAVSAQAPAAEPAREGFSETLEVQGVNVE
                     AVVTDKQGRRVPGLTARDFRLLVDGKPVHVDFFLEVREGRAAQTARLPGAAGEPEVAT
                     LEAGEAVPTNYLVFIDDYFSPLTLRNEVLKSLSRELRSLGARDRVAVVAFDGRGSRVL
                     SSWAPASEGLEHLLGQAGRQKRFLTRADLDLNTGPLPVGAAQPLGKGLMSEPVADAAG
                     DRSLRELEGIENVVAASAASLRAFADQPGRKVALVASGGWRVDQRAMMGLELPAYVKD
                     GYSLLDPLIDTANLLGYTLYPIHLAEKARLVPSAEPQLASAASGASTMELAAAQNSLV
                     LSARQTGGRLLMPGNNNYLQRVTEDTRSYYWLGFTHTGGDMRRVRLEVQALRPGVEVR
                     SRSSFLPLSRSAKVAMQVESAVLTGDLVGMEPLDVSLGRPVRVSRKALRVPVSLRIPM
                     KDITLLPNGSYQVARLELRTASTDKDGGRSLVAKVPLEIRIQGEAPPADGHLQHDLQL
                     GLRNVPQYVLVSIHDVASGKTLAARVPFEPEK"
     gene            4953..>5155
                     /locus_tag="VEL74_22580"
     CDS             4953..>5155
                     /locus_tag="VEL74_22580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65385.1"
                     /translation="MKRAAPPPSRRRLNAWGFEGEGLPVPRPMRKWLEERLGKSERRE
                     AVDPEGIAVPEPRPLPDLPGAVSV"
BASE COUNT          742 a         1860 c         1806 g          747 t
ORIGIN      
        1 cccgcccccg aggcggcgga gcccgagccc ggggtctcga gctcgtccta cgcccttctg
       61 gactgcccgg aaggcgtcgt ggcggaggtc gagttccccc tcaccgtcgg catctcgccg
      121 ctccccatgg tcccgggcga cgagccgctc gacctgccgc ccacccccgg gtacgtcctg
      181 gtcgtgcacg tcatcgccga gggcttccgg ctgcgggccg gggagccgga gcggcacgag
      241 ctcgcggtca cgcacgagaa tccctacccg accgtggtcc tccacctcac ccccgacccg
      301 caggacaccc gggtccggct gcggcgcatc cgggcgaact actcggtagg cggccaggcg
      361 atcggcttcg ccgaccgccc cctggtggtg gtggagagtg ccgagctgct ggactcgcag
      421 atccccaggc cggtcccgcg ccccggcacg atccacgtcc cgaccgccgg cacggcgccc
      481 gacctgaccc tcaccatccg cctcggcgat tcggtcggcg acctgctctg gacgctcgag
      541 agcccccacg ccatcgagct cccggaccgg cggctccaga gcgacatcgg caaggagccc
      601 gaccgcttcg cccgccagat cgtcgatctg gtcaacgggc gcgaagggac gaccggcctg
      661 tacacgttcc tggtcggcat cggccgggag gtcacgcagg aggtcccggt caaggtctgg
      721 aaggcgctcc gcgcggcggc cgcgaagcgg ggcggccccc ccgacctcct catcctctcg
      781 caggaaccct acatcccctg ggagctggcg gtgatgccgg agccgctcct cgatccccag
      841 gctccgccct tcctcgccgc ccaggccaac gtcgggcgct ggatcatccg caaggatctg
      901 gacgaagagg acggcggcag cctggaccgc cccaggctgc cgccgccgac cgagctggcg
      961 atcaaggggc tggccgtggt ctcgggcgtc tacagcagcg gcaagtggca gcggctgaag
     1021 gaggccgagc aggaggctgc cgagctggcg cagctctatc ccgcggcgtc cgtcaacgcg
     1081 gacttcgaaa ccgtgaccgg ctgcctcaag ggcatccccg aggcggacat gctccacttc
     1141 gcggtccacg gcgtctacaa cccggagagc gtggagcacg ggctgatgct ggtcgacaag
     1201 cgcgtcctcc accccttcga cgtcaagggg atcgacctga cgcgcgcccc cttcgtcttc
     1261 ctgaacgcgt gccaggtcgg cagcggcgag caggtcctcg gcgactatgc cgggctcgcc
     1321 gaggccttcc tcttcgccgg cgcttccggc gtgatcgccc cgctctggtc ggtcaaggac
     1381 acggtcgccc ggcaggtggc gctcgatttc taccgcgaga tcgccgcggg ccggacgccc
     1441 gccgacatcg tgcgcgccgc gcgggcccgg ttcaaggacg cggccgggcc gctgtcggcg
     1501 acctacctcg cctatcagtt cttcggccac ccgcagctca agctgaccgg gctccggcca
     1561 cgagaggagg gacagccgtg agcgagcgtc tcgatccgtc aggctcgccc gtcagggtgg
     1621 gcggcatcac cctgcgcacg ccgggattgg aaggggtcgt gagcgtcatg ccccccacga
     1681 gcgcggagac gcgggccgcc gcgttcgact ccgccgccct ggaggaggcg ctacgcgaag
     1741 aaggggtcgc caccgacgag acgctcgaga tcaccgacac caacgaggtc gaggcgggcg
     1801 gcggcgaggg gacgcggacg gcgcaggggg agccggcgat cgagctcgag gtctgggagc
     1861 cgggcgagga gtgggggcag atagtgctgt acaccgacga ggccggcgtg accacctggg
     1921 ccttctcctt gcccggagag gcggcagcgc cgggagagcg ggtgcgggga ggtgccggcg
     1981 gtcgcaccta tgtgctccgc cgccacgtcc ctcccgcgcc gcaaggggag gccacccggg
     2041 gcgtgatcgg cgccgccggc aagaagatca tccgggtgct ctccttcaag ctcctcgatc
     2101 cggtcttcgg cagggtgggg gagcacttcg tggccaagtg ggagcaggag aagcgcccct
     2161 accgcgtgcg cacgttcccg cccgagggct tccgcaacgc ctcggcgaag ctcgtcgacc
     2221 tccccgactg gccggcgctg gcgacgagcg acgggaagcg cgcgctgctc ttcgtgcacg
     2281 gcacgttcag ccgggcgcac gccgccttcg gcggcctccc cccggagttc atggaggccc
     2341 tgcaccgcag gtacgagggg cgggtcttcg ccttcgatca tccgacggct tcggaggacc
     2401 cccgccagaa cgtcgagtgg ctgctgcagg agatcccgga ccgcacccgg ctggacctgg
     2461 acatcatctg ccactcccgc ggcggcctcg tctcgcgcgt gctggccgag aagcaggcgg
     2521 atctcgcgct cgggtcgcgc caggtccggg tccacaatgt cgtcttcgtg gcggcgccga
     2581 acgccggcac gattctggtc aacgaagagc acatgggcga cttcgtggac tcctacacca
     2641 atctcctcaa ctttttcccc gacaacggcg tcaccgaggg gctggagtgg atcatcacgg
     2701 tcgccaagca tctcgcctgc tcgacgctca aaggcctcga ggggctccag tcgatgcggc
     2761 ccgggggcgg gtttctcacc gggatcaatg gcgcgaccag cccggacaag cgctactacg
     2821 ccgtcgcctc cgacttcgag cccgcggacc tcgggctgtc gtcgttcaag gacggggtga
     2881 tggaccggat cttcggccat ccgaacgatc tggtcgtccc cacggccggc gtatgggacc
     2941 acaacggcgg gtcgctcttc ccgatcgcgg agaagctcgt gctgcccgac aaggggatcc
     3001 atcacagcgg ctacttctcc tgtcccgagg tgcagcggca gatcgcgcgc tggctggagg
     3061 tcgagacggg ctaggcactc ctggcccgcg tcgtctccag aaggggactt cagcgtcacc
     3121 caccggcggc aatgacgttt tggatcgagg cgccggctga ggcgccgccc tctggcacat
     3181 cgcctgctag gtctggctac agacttcatc ttttccctcc gggaggacga aggatgcgag
     3241 cagtctgctg ggcagtttgc ctctccgtca ccctggccgg cgcggtctcg gcccaggcgc
     3301 ccgccgcgga gccggcgcgg gagggctttt ccgagaccct ggaggtccag ggtgtcaacg
     3361 tcgaagccgt ggtgaccgac aagcagggcc ggcgagtgcc ggggctcacc gcccgggatt
     3421 tccggctgct cgtcgacggc aagccggtgc acgtcgactt cttcctcgag gtgcgggagg
     3481 gccgggcggc gcagacggcg cggctgcccg gcgccgccgg cgagcccgag gtggcgacgc
     3541 tcgaagccgg tgaggcggtc cccaccaact atctggtctt catcgacgac tacttctcac
     3601 cgctgaccct gcgcaacgag gtcctgaagt cgctgtcgcg ggagctgcgc tcgctgggcg
     3661 cgcgggaccg cgtcgccgtc gtcgccttcg acggccgcgg cagccgcgtc ctctcctctt
     3721 gggccccggc ttccgagggg ctcgagcacc tgctcggaca ggcggggcgg cagaagcggt
     3781 ttctcacgcg ggccgatctt gacctcaaca ccggacccct gccggtggga gccgcccagc
     3841 ccctcggcaa gggtttgatg agcgagccgg tcgccgacgc cgccggagac cggtcgctgc
     3901 gggagctcga gggcatcgag aacgtggtgg cggcctccgc ggccagtctg cgcgccttcg
     3961 ccgatcaacc cggtcgcaag gtggcgctgg tggcctccgg tggctggcgg gtcgaccagc
     4021 gcgcgatgat ggggctggag ctgcccgcgt acgtcaagga cggctacagc ctgctggacc
     4081 cgctgatcga caccgccaac cttctgggat acaccctcta tcccatccat ctagccgaga
     4141 aggcccggct ggtgccgagc gccgagcctc agctcgcttc cgcggcctcc ggcgcctcga
     4201 cgatggagct ggccgcggcg cagaattccc tggtcctcag cgcccgccag accggcggcc
     4261 ggctgctcat gccaggaaac aacaactacc tgcagcgcgt caccgaggac acccgctcct
     4321 actactggct cggcttcacc cacacgggcg gtgacatgcg tcgcgtccgc ctcgaggtcc
     4381 aggcgctccg cccgggcgtc gaggtgcggt cgcggagcag cttcctgccg ttgtcgcggt
     4441 cggcgaaggt ggcgatgcag gtggagagcg cggtgctcac cggtgacctc gtgggcatgg
     4501 agcccctgga cgtctccctc ggccggcccg tgcgggtctc caggaaagcg ctacgggtgc
     4561 ccgtctccct gcgcatcccc atgaaggaca tcaccctgct ccccaatggc agctatcagg
     4621 tcgcccggct ggagctgcgc accgcctcca cggacaagga cggcgggcgc tcgctggtcg
     4681 ccaaggtgcc gctggagatc cggatccagg gcgaggcgcc gccggccgac ggccatctgc
     4741 agcacgatct ccagctcggg ctgcgcaacg tcccgcagta cgtgctggtc tccatccacg
     4801 acgtggcgag cggcaagacc ctggcggcgc gggtgccgtt cgagccggag aagtagccct
     4861 cacagatccg gagcctcccg ggagcgccgt ccggcgcttc cggggaggtt tcctgcgcaa
     4921 atccggacgc gatccgggct agactgcggg ccatgaaacg tgccgccccg ccaccgtccc
     4981 gccgccgcct gaacgcctgg ggtttcgaag gggagggcct gccggtgccg cgccccatgc
     5041 gcaaatggct ggaggagcgg ctggggaagt cggagcgccg ggaggcggtg gaccccgagg
     5101 ggatcgcggt gcccgagccg cgcccgctgc ccgatctgcc gggcgccgtc tcggt
//
LOCUS       DASPJI010000801         6275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_565718, whole genome shotgun sequence.
ACCESSION   DASPJI010000801 DASPJI010000000
VERSION     DASPJI010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565718"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="VEL74_22585"
     CDS             complement(<1..134)
                     /locus_tag="VEL74_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYG65386.1"
                     /translation="METIDLAIIGAGVAGLAAADELCRKGLTLAVFEARDRIGGRILT
                     R"
     gene            165..563
                     /locus_tag="VEL74_22590"
     CDS             165..563
                     /locus_tag="VEL74_22590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HYG65387.1"
                     /translation="MSHPIVLFDGVCNLCSGSVQFILKRDREGRLRFASLQSGAGRRL
                     LTEHGLDPDALSSVVVIEEGRAWQESSAALRIARHLPGAWQLLRALAVIPRPVRDTLY
                     RWIARNRYRWFGKTETCWLPTPELRARFLE"
     gene            590..1192
                     /locus_tag="VEL74_22595"
     CDS             590..1192
                     /locus_tag="VEL74_22595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65388.1"
                     /translation="MRTVIHNAGDYEITGLRLERVRRQDCLDLCLVRKFEHHVLRFWS
                     IRNLEIRRDDEAPRLTVLIDDGIDGELRVESPEGARSILRFDARSVERFARWPTVWDM
                     HIDEVAGLCLWAWRIPFVHYGVMNEEAGVPPALAKRLDRWIKSKRNGRPAEELDAEGR
                     ELAALVLPCLGPAAILYLYLEAEERSERIGAPGDWDVRVP"
     gene            1202..1696
                     /locus_tag="VEL74_22600"
     CDS             1202..1696
                     /locus_tag="VEL74_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019487410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthrone oxygenase family protein"
                     /protein_id="HYG65389.1"
                     /translation="MTDAGPWLFALTLVSALGCGLIAGAFFAFSSFVMSALGRLPPAS
                     GIAAMQSINIVVINPVFLGVFLGTAAACLGVLISAFLRWPAPGTSYAIAGSALYIAGT
                     FLVTMAFNVPRNNMLAAVDPASHGGARVWAGYLVTWTRWNHVRTAAALAAAALLIMAL
                     VQAG"
     gene            complement(1703..4129)
                     /locus_tag="VEL74_22605"
     CDS             complement(1703..4129)
                     /locus_tag="VEL74_22605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG65390.1"
                     /translation="MSLLRDAHFGTRVLLRRPGFAAAVVLTLALGTGATTAIFSIVNA
                     ALLRPLPYREPDRLMMVWERNVSLDAEANVVSPFNFMAWKAGTRSFSGLDAFVARSGT
                     LTGQGKPQWLRLGLVSPGFFSTVGVEPILGRTFSPEEAVPGGPLTAVLGHGLWRSTFG
                     GDPAVVGRTIRLNDIEVTVLGVLPQGFELLEPLDLWIPLAINPAQPGRGRFLTVVGRL
                     GSGVSEAQAQAEMDALARTRVQQEVDYLTGWDVNVMPLREHLTGDSRDTLLMLLGAVV
                     FVLLIACANVANLFLSRATTRQREVAIRAALGADRSRLIRQMLTESTLTALAGGVAGV
                     AVAWLGTRLLARLAPESLPFLASVGMDWRVLLFAAGVILAVAILSGLAPAMVLMRPDL
                     REDLKEGGKATEGQGRLRLRHLLVVTDVAMAVVLLVGSGLMLRSLQLLQSVDLGYEPD
                     HLLTFRIGMPEQRYPGADPKAAFTERVLETIEAMPGVQSVSAGSILPMGGAGIGHAME
                     VDGRPKPAPGQEIPVDLRFVTRGYFRTLGIPLVAGRDFTSRDRRGSPHVIVIGQHTAR
                     TVWPGEPALGKRIHMDWLEVVDGEVQEVRITATVVGIVGDIREKGFESEPETMLYWPL
                     RQMSTNPIHVAVRSAADPAALATTIRNAVESLDPDLPIYEVATMEEQLARSIAQRRFS
                     MALLTLFSALALVLAAIGIYGVLSYAVNLRTREIGLRIALGSARSSILRLIVGQGLRL
                     ALLGLAVGLPLAYALAKLLASLLYKVGLTDPMVYGAAPVLLLGVVLLACYLPARRAAR
                     LDPMVALRSE"
     gene            complement(4751..5599)
                     /locus_tag="VEL74_22610"
     CDS             complement(4751..5599)
                     /locus_tag="VEL74_22610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65391.1"
                     /translation="MSDGRRLRVEVPEASPFFDGHFPGHPILPGIAHLLLAAWGLGPI
                     SGPSPLGGGGLDQGGGQEGGAPVTTAPFTAPLAEVRNLRLRRPVGPGDLLDLHLDAPS
                     EDGTARFELRRGAESLSQGTVRLATEPEEALLPGAPAFPAGAGFPAPGALVPHAPPAL
                     LVRTVLEAAAEGAACVVEIPAASPFAAGGRAPAFLGLEAAAQTAAVLESLARTDASGP
                     RLGYLVGVRDARFHTPWLPVDHPLRATVRLTGSAPPLAIYETRIDTGDGRELVTGTVS
                     TYVTAK"
     gene            complement(5596..>6275)
                     /locus_tag="VEL74_22615"
     CDS             complement(5596..>6275)
                     /locus_tag="VEL74_22615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65392.1"
                     /translation="PIDERRTSIGLVMDADAARSVGVPAQRMLAWGIARCPAMRQRTA
                     EAVFPAGNNVIADFSYRCSPYAGPGYFLVGDAATFIDPIFSTGVCLGMMSAIQAAGGI
                     GALLRGADPGRIRRDYIRFIEKSSEPFFGLVDAYYQSPFRDLFMAGQGPFQVHRAILS
                     VLAGNVFPRPPFPLRWRLGLMHLLARLQRFVPLAPRRPRFSLLADPAETPETSEAVSL
                     PRKMSAG"
BASE COUNT          949 a         2130 c         2274 g          922 t
ORIGIN      
        1 cgcgtcagga tgcgcccgcc gatgcggtcg cgcgcttcga aaaccgcgag ggtgagcccc
       61 ttccggcaga gctcgtccgc cgcggcgagg ccggccacgc cggcgccgat gatggcgagg
      121 tcgatcgtct ccattcgggt atgatctcat ctggacccat ggagatgagc cacccgatcg
      181 tgctcttcga cggcgtctgc aatctctgca gcggctcggt ccagttcatc ctgaaacggg
      241 accgggaggg gcggctccgc ttcgcctcgc tccagtccgg ggccgggagg cgcctgctga
      301 ccgaacacgg gctcgatccg gacgccctga gcagcgtggt cgtgatcgag gaggggcggg
      361 cctggcagga gagctcggcg gcgctacgga ttgcccgcca cctgccgggg gcgtggcagc
      421 tgctgcgggc cctcgcggtg atcccccggc ccgtccgtga caccctctat cgttggatcg
      481 cccgcaaccg ctatcgctgg ttcggcaaga cggagacctg ctggctgccg acgcccgagc
      541 tgcgggcgcg atttctcgaa tgactcaaaa acctgaagac tcgagcgtat tgcgcaccgt
      601 gatccacaac gcgggcgact acgagatcac gggcctgcgg ctggagcgcg ttcgccggca
      661 ggattgcctc gatctctgcc tcgtccgcaa attcgagcat cacgttctcc gcttctggtc
      721 gatccggaac ctcgagatcc gccgcgatga cgaggccccc cgtttgaccg tgctcatcga
      781 cgacggaatc gatggcgagc tgcgggtgga gagccccgag ggtgcgcgat cgatcctccg
      841 tttcgacgcg cggtcggtcg agcgcttcgc tcgctggccg actgtctggg acatgcatat
      901 cgatgaggtc gcgggcctgt gtctgtgggc ctggaggatc ccgttcgtcc actacggcgt
      961 catgaacgag gaggccggag tgccacccgc gctggcgaag cggctcgatc gatggatcaa
     1021 gagcaaacgc aacgggcgtc cggccgagga gctcgacgcc gaaggccgcg agcttgccgc
     1081 tctcgtgctt ccttgcctcg gccccgctgc catcctctac ctttatctgg aagccgagga
     1141 gcggtctgag cggatcggcg ctccgggaga ctgggacgtc agggtcccat agggttattt
     1201 catgacggat gcgggaccct ggctcttcgc gttgacgctc gtctcggctc tcggctgcgg
     1261 cctgatcgcc ggtgctttct tcgctttttc ttccttcgtc atgtcggccc tgggccgtct
     1321 gcccccggct tccggcatcg ccgccatgca atcgatcaac atcgtggtga tcaatccggt
     1381 tttcctgggg gtcttcctgg ggaccgccgc cgcctgcctg ggtgtgctga tctccgcgtt
     1441 cttgcgctgg cccgcgccgg ggacctcgta cgcgatcgcg gggagcgcgc tttatattgc
     1501 cggcacgttc ctggtgacga tggccttcaa cgtgccgcgc aacaatatgt tggccgccgt
     1561 cgatcccgcg agccacggcg gcgccagggt gtgggccggc tatctcgtca cctggacgcg
     1621 gtggaaccat gtgcggaccg ccgcggcgct cgccgcggcg gccctgctca ttatggccct
     1681 ggttcaggct ggatagccgc cgtcactccg accgcagcgc caccatcggg tccagcctcg
     1741 ccgcccggcg cgcgggcaga tagcaggcga ggaggacgac gccgaggagg agcaccggcg
     1801 cggcgccgta gaccatcgga tcggtgaggc ccactttgta caggaggctc gcgaggagct
     1861 tcgccagcgc ataggcgagg gggagcccca cggcgagccc gagcagggcg agccgcagcc
     1921 cttgtccgac gatcaaccgc aaaatgctgc tccgggcgct gcccagggcg atgcggagcc
     1981 cgatctcccg cgtccgcagg ttgaccgcgt aggagaggac gccgtagatc ccgatcgcgg
     2041 ccagcaccag ggccagcgcc gagaacagag tgagcagcgc catggagaag cggcgctggg
     2101 cgatggagcg ggcgagctgc tcctccatgg tcgccacctc atagatgggc aggtccgggt
     2161 cgagcgactc gacggcgttc cgaatggtgg tggcgagcgc cgccggatcg gcagcgcttc
     2221 gcacggccac gtggatcgga ttcgtggaca tctgccgcaa cggccagtac agcatcgtct
     2281 caggctcgct ctcgaacccc ttctcccgga tgtcaccgac gatccccacc accgtggccg
     2341 tgatccggac ctcctggacc tcgccgtcga cgacctccag ccaatccatg tggatccgct
     2401 tgccgagggc cggctcgccc ggccagaccg tgcgggcggt gtgctgtccg atgacgatca
     2461 cgtgcggaga gccgcgccgg tcccgggagg tgaagtcccg gccggcgacg agggggatgc
     2521 ccagggtccg gaagtagccg cgggtcacga agcgcaggtc gaccgggatc tcctggccgg
     2581 gcgcgggctt cggacgtcca tcgacctcca tggcgtggcc gatcccggct ccgcccatgg
     2641 gcaggatgct cccggcgctc accgactgga cgccgggcat ggcctcgatc gtctcgagga
     2701 cccgctcggt gaaggccgct ttcgggtcgg ctccggggta gcgctgctcc ggcatgccga
     2761 tccggaaggt gaggaggtga tccggctcat agcccaggtc gacgctctgc aggagctgga
     2821 ggctgcgcag catcagcccc gacccgacca gcaggacgac cgccatggcc acgtcggtga
     2881 cgaccagcag gtggcgcaga cgcagccgcc cctgtccttc cgtagccttg ccgccctcct
     2941 tgagatcctc ccgcaggtcc ggacgcatca ggaccatcgc cggcgccagc ccggacagga
     3001 tggcgacggc gaggatcacc cccgcggcga agagaaggac gcgccagtcc atccctaccg
     3061 aggccaggaa gggcaggctc tcgggcgcca ggcgggccag caggcgcgtc cccagccagg
     3121 cgaccgcgac gccggcgact ccgccggcca gcgcggtgag ggtgctctcg gtcagcatct
     3181 gccggatgag acgggaccgg tccgcgccga gcgcggcgcg gatcgccacc tcccgctggc
     3241 gcgtggtggc ccggctgagg aagaggttcg cgacgttggc gcaggcgatg agcaggacga
     3301 agacgaccgc cccgagcagc atcagcagcg tgtcccggct gtcgcctgtc aggtgctcgc
     3361 ggagcggcat cacgttcacg tcccatcccg tgaggtagtc cacctcctgt tgcacccgcg
     3421 ttcgggcgag ggcatccatc tccgcctggg cctgcgcttc gctcacaccc gacccgagcc
     3481 ggccgaccac ggtgaggaag cggccccggc cgggctgggc cgggttgatg gcgagaggta
     3541 tccacaggtc caggggctcc aggagctcga agccctgcgg caggactccg agcaccgtca
     3601 cctcgatgtc gttgagccgg atcgtccgcc cgaccacggc cgggtccccg ccgaaggtgc
     3661 tccgccacag gccgtgcccg aggaccgccg tgagaggacc gccggggacg gcctcctcgg
     3721 gcgagaaggt cctgcccagg atcggctcga cgcccacggt cgagaagaac ccgggggaga
     3781 ccaggcccag ccggagccac tggggcttgc cctgcccggt cagcgtaccg ctccgggcga
     3841 cgaacgcgtc caggccggag aacgaccggg tccccgcctt ccaggccatg aagttgaagg
     3901 gggagaccac gttcgcctcc gcgtcgaggc tgacgttgcg ctcccagacc atcatcagcc
     3961 ggtccggctc gcgatagggc aggggccgca ggagcgcggc gttgacgatc gagaagatgg
     4021 cggtcgtcgc ccccgtgccg agggcgaggg tgaggacgac ggcggcggcg aatcccggcc
     4081 gccgcagcag cacgcgcgtt ccgaagtggg catctcgaag caggctcatc cacaccctcc
     4141 ttgtagagcg acccgaggtt gacgagtata cgtactaccc gggtagtttg ttgcggccgt
     4201 tccttcgcct tcgtcctcgc gtgcggtctc ccggccggcg gagtgaccgg gcggacgctc
     4261 gtccggctga ggccggtctg acgacggccg catcgcccgg tccgtctatc tataaaagga
     4321 agcagcgcct tctccgccgt cgcgggggag gcgcttttct cttggcggag actccgacgg
     4381 cgcgtcggag tctccggcgg cggattggaa gcttccgttg gcgcgtcgga gactccgata
     4441 gcccgccgga accttcgaca gcccaccgaa gactccgata gtctgttgga agcttcgaca
     4501 gggcgccgga agcttcgaca gcgcgttgga agcttccgac agcccgtcgg agattccgac
     4561 ggcgcgtcga aggctacgac ggcgtatcgg agagcccgat agcgtgccgg aaccttcggc
     4621 agcccgccga agactccgat ggcccgttgg aagcttcgac agctcatcgg aaactccgct
     4681 ggcccgtcga gctctccgac agcgcgccga agactccgac ggcgcgtcgg agactctttc
     4741 gagacaccct ctacttcgcg gtgacgtagg tgctgacggt gccggtgacg agctcgcggc
     4801 cgtcgccggt gtcgatccgc gtctcgtaga tggccagcgg cggggcgctg ccggtgaggc
     4861 ggacggtggc gcggaggggg tggtccaccg gcagccaggg ggtgtggaag cgggcgtcgc
     4921 ggactccgac gaggtagccc aggcgcgggc cggaggcgtc ggtgcgggcc agggattcca
     4981 ggaccgcggc ggtctgggcc gcggcctcga ggcccaggaa ggcaggggcc cggcctcccg
     5041 ccgcgaaggg gctggcggcg gggatctcga ccacgcaggc cgcgccctcg gccgcggctt
     5101 cgaggacggt gcgcaccagg agggcgggcg gcgcgtgggg caccagggct ccgggggccg
     5161 ggaagccggc gccggccggg aaggctggcg cgccggggag gagggcctcc tccggctccg
     5221 ttgcgaggcg gaccgtcccc tggctcaggc tctccgctcc ccgtctcagc tcgaagcggg
     5281 cggtcccatc ctctgaaggc gcatcgaggt ggaggtcgag gaggtcgccg ggacccaccg
     5341 ggcggcgcag ccggaggttg cggacctcgg cgagcggggc ggtgaagggg gcagtggtaa
     5401 cgggggcgcc cccctcctgt cctcccccct gatccaaccc tccccccccc aggggggagg
     5461 gacccgagat cggacccagt ccccaggcgg cgaggaggag gtgggcgatg ccggggagga
     5521 tggggtggcc ggggaaatgg ccgtcgaaga aggggcttgc ctccgggacc tcgacacgga
     5581 ggcggcggcc gtcgctcatc cggcgctcat cttcctcggc aatgagaccg cctcgctggt
     5641 ctccggggtc tccgcgggat ccgcgaggag cgagaagcgc ggccggcgcg gcgccagcgg
     5701 cacgaagcgc tggagacggg ccaagaggtg catgagcccg aggcgccagc gcagcgggaa
     5761 ggggggccgg gggaagacgt tccccgccag caccgagagg atcgcccggt gtacctggaa
     5821 cggtccctgg ccggccatga acaggtcgcg gaacggcgac tgatagtagg cgtccaccag
     5881 gccgaagaaa ggctccgagc tcttctcgat gaagcggatg tagtcccgcc ggatcctccc
     5941 cgggtcggcg ccgcggagca gggccccgat gccgccggcg gcctggatgg cgctcatcat
     6001 ccccaggcag acgccggtgg agaagatggg gtcgatgaaa gtggccgcgt cccccaccag
     6061 gaaatagccg gggccggcat agggggagca gcgatagctg aagtcggcga tgacgttgtt
     6121 gcccgcgggg aagaccgcct ccgccgtgcg ctgccgcatc gccgggcagc gcgcgatccc
     6181 ccaggccagc atgcgctgcg ccggcacccc gacgctgcgg gcggcgtcgg cgtccatcac
     6241 caggccgatg ctggtgcgcc gctcgtcgat cggga
//
LOCUS       DASPJI010000802         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_795660, whole genome shotgun sequence.
ACCESSION   DASPJI010000802 DASPJI010000000
VERSION     DASPJI010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795660"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1948)
                     /locus_tag="VEL74_22620"
     CDS             complement(<1..1948)
                     /locus_tag="VEL74_22620"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG65393.1"
                     /translation="MSVPDLSSGLASRLEALTPAQRALFELALRKQAAASVPAVQRTI
                     PRRACFSPCVLSFDQERLWFVQQLDTSSPIYNVYQATRYRFVMEPLTLARALNEVVRQ
                     QESLRTTFQTIDGQPMQVIAPAYEINLPWIDLRSLPAERREEEVLRASTQVVSPAFDL
                     GRLPLVRIVLIQVEEEDYVNPVCMHHLVTDWISTYTLLTGIMETYNALRAGLPSPLTE
                     LPVQYGDFAEWQRARLSDPVLVEQMVAYWRKRLADAPELLPLPYDRPRPVMQTARGRR
                     TLLTLSAAHSTGLRGLAQRHEATLFIVLLAALKALLVRVTGEEKLIVGTPSAHRNLPE
                     LERVVGFFLNQLALYTDLTGNPSLLEVVRRVRETAMGAYAHEEMPFAKLVETLRPQRD
                     ISRTPFTQVVLLLLAPHVAMERRNLTGGDGVENYWVDAHRTQFDFNLSFWEATDGSFL
                     SGWWEHSTDLFDSTTIDRLKDQLRRVVAALVEDPETRLWDVPLLSAAQSHQLLLEWGG
                     EGVAATGPAVHERFAAQAARSPAATALVAGERLLTYDELNRWANRLAHQLRRLGVGPE
                     RVVGLCLERSPELVAGILGVLKAGGAYLVLDPALPDARLSFQMSDAQPAVVLAGPGLT
                     GRQDEIATAGKRVEPLVELAATAQP"
     gene            complement(1945..>2232)
                     /locus_tag="VEL74_22625"
     CDS             complement(1945..>2232)
                     /locus_tag="VEL74_22625"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYG65394.1"
                     /translation="LAGNSLLAIQIVTRISGALGVNLPMASLLESPTVAELAARAEVL
                     RPAAPTIPLAAAGAMPESELDRLLREIESLSPEEAEVRLAGELAVGTEVDR"
BASE COUNT          354 a          757 c          806 g          315 t
ORIGIN      
        1 cgggttgggc cgtggcggcg agctccacca gcggctcgac ccgtttgccg gcggtggcga
       61 tctcgtcctg gcgtccggtg aggcccggac cggcgagcac cacggccggc tgggcatccg
      121 acatctggaa ggacaaacgc gcgtctggca gggcaggatc gagcacgaga taggccccgc
      181 cggccttgag cacgcccagg atgcccgcca ccaattccgg cgagcgctcg aggcagagtc
      241 ccaccaccct ttccggcccc actcccagcc ggcggagctg gtgggccaga cggttcgccc
      301 accggttcag ctcgtcgtag gtgagcaggc gctcgccggc gacgagggcc gtggccgcgg
      361 gcgaacgcgc cgcctgggcc gcgaaacgct cgtggacagc ggggccggtg gcggccaccc
      421 cctctccccc ccactcaagg aggagctgat ggctctgcgc cgccgacagc agcggcacgt
      481 cccacaaccg ggtctccggg tcctccacca gggcggccac gacccgccgg agctggtcct
      541 tgagacggtc gatggtggtg ctgtcgaaca ggtcggtgct gtgctcccac cagccggaaa
      601 ggaaggagcc gtcggtcgcc tcccagaacg acaggttaaa gtcgaactgc gtccggtggg
      661 cgtcgaccca gtagttctcg accccgtcgc cgccggtgag gttgcggcgc tccatcgcga
      721 cgtggggggc gaggagcagt agcaccacct gggtgaaggg ggtgcgggag atgtcgcgtt
      781 gcggtcgcag ggtctccacg agcttggcga acggcatctc ctcgtgggcg tacgccccca
      841 ttgccgtctc ccgcacccgc cgcaccacct ctaggagaga cggattgccc gtgagatcgg
      901 tgtagagggc gagctggttg aggaagaagc cgacgacccg ctccagctcg gggaggttgc
      961 ggtgggcgct gggggtgccc acgatgagct tctcctcgcc ggtgacccgg accagcaggg
     1021 ccttgagcgc ggccaggagc acgatgaaga gcgtcgcctc gtgacgctgc gccaggccgc
     1081 gcaggccggt gctgtgcgcc gccgatagcg tcagcagggt gcgccgtccg cgcgcggtct
     1141 gcatcaccgg ccgcggccgg tcgtagggca gcggcaggag ctccggcgcg tccgccaggc
     1201 gcttccgcca gtaagccacc atctgctcca ccagcaccgg gtcggagaga cgggcgcgct
     1261 gccactcggc gaaatcgccg tactgcaccg gcaattcggt caggggcgac ggcaggccgg
     1321 cgcgcaaggc gttgtacgtc tccatgatcc cggtgagcag ggtgtaggtg gagatccagt
     1381 ccgtcaccaa gtgatgcatg cagaccggat tgacgtagtc ctcctcctcc acctggataa
     1441 ggacgatccg caccagcggc agcctgccga ggtcgaaggc gggactgacc acctgggtcg
     1501 aggcgcggag gacttcctct tcccgccgct ccgctggcag agaccgcagg tcgatccacg
     1561 gcaggttgat ctcgtacgcc ggcgcgatga cctgcatcgg ctgaccgtcg atggtctgga
     1621 acgtggtgcg caggctctcc tgctggcgca ccacctcgtt gagcgcccgc gccagggtca
     1681 agggttccat gacgaagcga tagcgcgtcg cctggtagac gttgtagatc gggctcgagg
     1741 tgtcgagctg ctgcacgaac cagagccgct cctggtcgaa ggagaggacg caggggctga
     1801 agcacgcccg ccgcgggatg gtgcgctgga cggctgggac cgacgccgcg gcctgcttcc
     1861 ggagcgccag ctcgaacagg gcgcgctggg ccggcgtcag agcctcgagg cgggaggcga
     1921 ggcctgagga gaggtccggg acgctcaccg gtccacctcc gtgccgacag cgagctcgcc
     1981 ggcgagccgc acctccgcct cctcggggga gagcgactcg atctcgcgca gcaggcggtc
     2041 cagctcactc tccggcatcg cgccggccgc agccagcggt atcgtcggag ccgccggccg
     2101 aagtacttcg gcacgcgctg ccaactcggc aaccgtcgga gactccagca gactggccat
     2161 tgggagattg acccccagcg cgccagagat ccgggtgacg atctggatcg ccagcagcga
     2221 gttgccggcg ag
//
LOCUS       DASPJI010000803        13067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_795721, whole genome shotgun sequence.
ACCESSION   DASPJI010000803 DASPJI010000000
VERSION     DASPJI010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13067)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13067
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795721"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VEL74_22630"
     CDS             <1..310
                     /locus_tag="VEL74_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HYG65395.1"
                     /translation="IDVLEQFAADPGTEGVIMIGEIGGSAEEAAAEWVQANMDKPVVG
                     FIAGRTAPPGRRMGHAGAIVAGGKGTAAEKMEAMRAAGIHVVESPAELGSTMAKALGK
                     "
     gene            329..1054
                     /locus_tag="VEL74_22635"
     CDS             329..1054
                     /locus_tag="VEL74_22635"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HYG65396.1"
                     /translation="MNGNGFGRRGWLALIGGGEFSFEETLDADEAWVEKLPPGPVGFI
                     PAASGSNDYAHHFAEYMGEAFQRQVEVIPIYRARDARRGLNSERIRDVAAVYIGGGVT
                     DHLLDALRDTPATEALATKIRDGGVVVAIAAAAQAAGRLARSIFRGDAIPGFGWLPDA
                     VVEPNFDPAKDRRLRKLLAAAGAPGVNWGLGIPAGNAVLLGPEGVMEVVGTAFRLGAP
                     DAGLEVLGDPDFEIASDEDLEEE"
     gene            1147..1569
                     /gene="ndk"
                     /locus_tag="VEL74_22640"
     CDS             1147..1569
                     /gene="ndk"
                     /locus_tag="VEL74_22640"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007546394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HYG65397.1"
                     /translation="MDTTLAILKPDSVEAGSAGKILAHLQNEGFTIRGIKLMRLTQAQ
                     AQAFYEVHKERPFYGSLVEFMTSGPVMPVALERDNAVAHLRQVMGATDVAKAEEGTVR
                     KLFGTSIERNAIHGSDSPENAAIELSFFFSRAELQAAR"
     gene            1588..1881
                     /locus_tag="VEL74_22645"
     CDS             1588..1881
                     /locus_tag="VEL74_22645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65398.1"
                     /translation="MYREPQAAPESQLPEERRGRTIGRFCLTCSSIYPLHRLRHDGKP
                     MYGKDHVAAPCAHEGDNFAPGEGWWEPAVLVLPQAAPAPEGQFTNAPTKGTQP"
     gene            1909..3579
                     /locus_tag="VEL74_22650"
     CDS             1909..3579
                     /locus_tag="VEL74_22650"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HYG65399.1"
                     /translation="MSVFSPRPEAAPVYGAAIAQGRLALWVTWLRQGRGNALGIALAA
                     GLLGELVSGWGAPPLVPTLPFTPFVSCAAGLLLGWQGVAATSAGQFLAIWMTRRNPLE
                     AAILAVCLALLGVAAHLVFRSIPKLGRGLPNLRSYLWLLGSALFGGLVAAGIGNLLLP
                     TQPIDTTWIWNRAAGSLVSVLLLVPPLLLVADRHLRPWIMPIPGEMPARRVRRFGEDV
                     ASEAVSAEATVMLERSRPPEVPKGVLVGAGLVLGVSVLVVPVISLIPEVGGWLRLFYL
                     IPILWAALAFGLRGGVLAASASGFAYLLGIAWMAAAVEPAESARALWTAYADLFALGL
                     TGAFVGRSREREFHLRQELADTNRLLRRDILRVAQALTQAVQAKDEYTEGHLHRVSEY
                     AVTVGERLGLRGQDLEMLHYASLLHDIGKIGIPEQVLKKPGRLEEAEAEAMRRHPEIG
                     ARMIENLDVLSGAAPLVLHHQERYDGRRNGEYPGYPGGLAGDRIPLGARIIAVVDAFD
                     AMTTNRPYRRALSPQHAIAVLQKERGQQFDPRVVDAFVSLLAERPWEE"
     gene            3567..4226
                     /locus_tag="VEL74_22655"
     CDS             3567..4226
                     /locus_tag="VEL74_22655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HYG65400.1"
                     /translation="MGRVDGGLDWEAVDRHLVEVRGCRKCPGVVPPPVAARPSQWPRV
                     MLVGQAPGPKEQEVGRPFAFTAGTRLFSWFAALGVSEEEFREKVWMTAVIRCFPGRAP
                     QGGDRVPAPAEIANCWPYVERELRLIRPATVLAVGQLAIRHLLPDPAPLNERVGRVFT
                     SSREDLEFDVIPLPHPSGRSTWLVRPENRDLLDRALRHLAESRGWRETFGERGTSILE
                     V"
     gene            4322..4492
                     /locus_tag="VEL74_22660"
     CDS             4322..4492
                     /locus_tag="VEL74_22660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65401.1"
                     /translation="MSANTTAVERLEMIWPLTVDAWALAGRIIPDYPRHEAPIRVIHG
                     HIRKQSREGEPA"
     gene            4513..4947
                     /locus_tag="VEL74_22665"
     CDS             4513..4947
                     /locus_tag="VEL74_22665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008655045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65402.1"
                     /translation="MAALVAENAVFLVVGAHAVGVHGVPRATGDLDILIRPDAENGKR
                     VWRALVQFGAPVEVTGLTPTDLEKPDMVYQIGLPPRRIDILTEISGVDFEEAWSSRVT
                     SRVGDLEIPFIGKDALLRNKRASGRPKDLADIDILERREPSE"
     gene            4954..5229
                     /locus_tag="VEL74_22670"
     CDS             4954..5229
                     /locus_tag="VEL74_22670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65403.1"
                     /translation="MDTTPAEQAVLARFRERCHIAGVVQVGYGMRAAAIRLARNSRPE
                     LDVDQALTAMVEKGWLKRNEAGTWYYLTADGAEQVKAPAPLSALQVT"
     gene            complement(5236..6789)
                     /locus_tag="VEL74_22675"
     CDS             complement(5236..6789)
                     /locus_tag="VEL74_22675"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HYG65404.1"
                     /translation="MRGLLIFISFSLLAIPTLAAETGTVVVGSKNFEESRLLGEMFAQ
                     LLESRTGLRVERRLGLAGTQVCFEALRTGAIDVYPEYTGTGLVTLLGEEPEGGPAETL
                     NRVRREFLARWDLWWIAPLGFENAFEVALPRELARREGIETISDLARAAPRLRAGFGH
                     EFVGREDGLLGLQRVYGLRFASVQPMQHALKYQAAGSRAIDALDVYTTDGRLVLYNLQ
                     VLEDDKGFFPPYDAAALVRGATLREHPEVSAVLGLLAGALDEETMRRLNVRLQEGKES
                     EAAVARDALRALGLLEGAAAGEEAAPRRPGFLSYAWSERGGLARRTLEHLGLCAAALL
                     LGILVAVPLGLWLERHRRGAEPAIRALGLTQTIPSLALLAFMVPFLGVGFVPALAALW
                     LYSLFPMVRNTYTGVRESDPRAVEAATALGMTPGQVLRQIRLPLAAPVIMAGVRTAAV
                     ITVGTATLAAFIGAGGLGEPIVTGLQLADTAMILSGAVPAAVLALLVDGGLALLERAF
                     HPGGGPEGR"
     gene            complement(6786..7541)
                     /locus_tag="VEL74_22680"
     CDS             complement(6786..7541)
                     /locus_tag="VEL74_22680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HYG65405.1"
                     /translation="MSSLPAGPALEATGVAKTWPDGTPALAGVDLAVQPGETVALVGE
                     SGSGKTTLLRLFNRMVEPTAGEVRVEGEPVARLDPITLRRRIGYVPQDGGLLPHWRVE
                     RNVELVPRLLGWDPERRRQRTREMLDLVGLDPAQYGRRYPAELSGGQRQRVAFARALA
                     ANPDVILLDEPFGALDALTRLDLHRQFLDLEARLRKTALLVTHDLGEAFRLADRIGVM
                     RQGRLLQLGPPEELSRSPADPYVRALLEMRGSG"
     gene            7596..8816
                     /locus_tag="VEL74_22685"
     CDS             7596..8816
                     /locus_tag="VEL74_22685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYG65406.1"
                     /translation="MRRETLRFPGAQGADLAARLESPDVDPTAYALFAHCFTCSKDLK
                     AAGWISRALVERGIAVLRFDFTGIGESEGDFAGTDFSGNVADLVAAADFLRTRGEAPR
                     LLVGHSLGGAAVLAAAVRIPETAAVATIAAPSDTEHLRDKLVRLAPELEERGEAEVDL
                     GGERPFPIRRQLLDDLAEDHLQEALHQLHKPLLLFHSPVDNVVGVEHAHRLFEAVKHP
                     KSFISLGLADHLLTDERDARHVGETLAAWAGRYLEGSQPEIETLPEAGRTGEVVVTER
                     GRFAQDIVARRHRLTADEPESVGGGDTGPTPYDLLLAAVGACTSMTLRIYADHKKLPL
                     EGVRVRLRHSKVHATDCAVCETKEGKLDQIERVIELLGPLTDEQRQRMLEIANRCPVH
                     RTLESEIHVPTRLE"
     gene            9223..10518
                     /gene="pcnB"
                     /locus_tag="VEL74_22690"
     CDS             9223..10518
                     /gene="pcnB"
                     /locus_tag="VEL74_22690"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HYG65407.1"
                     /translation="MSDGDELEPRVVPRSEHPISRRQISSNALKVMYRLHNARYRAYL
                     VGGSVRDLMLGREPKDFDVGTDARPNDVRRLFSNSRIIGRRFRLVHVFFHDGETVEVS
                     TFRRNPEPEEQTSAPGELLITSDNTFGTPREDAFRRDFTINALFYDISSYSVIDYCGG
                     IEDLERRLIRAIGDPDVRFQEDPVRMLRACEFAGRLGFGIETRTQEAIHRHRKELDKA
                     SPARVTEEIAQLLRCGRAGAAMQWMLELGLLEVLLPEAYAMLTAGRRGLGDFGQLLPV
                     IDRMVAEGRQLTDGALLAAILLPKILLRRDDIEAEQRRPMSRAAIGTMIEEEIQPFLS
                     RLTVSNLKSTQILQALIGFQRLCEPSWTPGERVRFSRKSFFDNSLLLFEMLVEATGEG
                     GEALAEWQSAARRRSAPPSAEGARRSRPRRRRRRQGQAL"
     gene            10589..11284
                     /locus_tag="VEL74_22695"
     CDS             10589..11284
                     /locus_tag="VEL74_22695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HYG65408.1"
                     /translation="MSWFTDLTRSAVGKKAVMAVTGIILFGFVLIHMIGNLKLYGGKD
                     AEGVYHLDSYGGWLREMGSPAVPHSGLLWIARLVLLASVVLHIWSSWAVTRMSRDARP
                     HAYAKRKPVRMTYASRTMRWGGVIILLFVIYHLLHFTTGNVHPDFREGAVFHNVVTGF
                     QNPLVSLFYIVANVALGFHLYHGLWSLFQSLGWNHPRFNHWRRGFAHAFAWIVTLGNI
                     SFPLAVLAGIVRL"
     gene            11297..>13067
                     /locus_tag="VEL74_22700"
     CDS             11297..>13067
                     /locus_tag="VEL74_22700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HYG65409.1"
                     /translation="MKLESKAPTGPLETRWQEHKDHLRLVNPNNKRRFTILVLGSGLA
                     GASAAASLAELGYNVKCFCFQDSPRRAHSIAAQGGINAAKNYHNDGDSVFRLFYDTVK
                     GGDFRSRESNVHRLAELSVSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVARTFYARG
                     QTGQQLLLGAYQALSKEIASGRVKMYPRTEMLDLVVDNGRALGIVARDMVTGKIESHA
                     GHAVVLATGGYSNVYYLSTNAKGSNVTASWRAHRRGALFANPCYTQIHPTCIPASGDY
                     QSKLTLMSESLRNDGRIWVPRDPNDQRSPDQIPDADRDYYLERKYPSFGNLVPRDVAA
                     RNAKIMVDEGRGVGPQKNGVYLDFSASIQRLGKAVIAERYGNLFEMYEKITGEDPYVV
                     PMRIYPAPHYTMGGLWVDYNLMSNIEGLYVLGEANFSDHGANRLGASALMQGLADGYF
                     VIPYTIGDYLAPHLGEPISTDGAAFRATEAEVLDRIKRLTSAAGRRSPDSYHRELGRI
                     VWDYCGIARNAGGLRKALQMIPALREDFWANVRVMGKVDDLNQDLERALRVADYFELA
                     ELMCLDALDRDESCGGHLREEHQT"
BASE COUNT         2021 a         4599 c         4562 g         1885 t
ORIGIN      
        1 catcgacgtg ctggagcagt tcgccgccga cccaggcacc gagggggtca tcatgatcgg
       61 cgagatcggc ggcagcgccg aagaggccgc ggccgagtgg gtccaggcca acatggacaa
      121 gcccgtggtc ggcttcatcg ccggccgcac cgccccgccg ggacgccgca tggggcacgc
      181 cggcgccatc gtggccggcg gcaagggcac ggcggccgag aagatggagg ccatgcgcgc
      241 cgccggcatc cacgtcgtcg agtccccggc cgagctcggc tcgaccatgg cgaaggcgct
      301 ggggaagtag agacgtcagg gcgcgaggat gaacggcaac ggattcggcc ggcgtggctg
      361 gctcgccctc atcggaggcg gcgagttcag cttcgaggag acgctggacg ccgacgaggc
      421 gtgggtggag aagctgccgc ccgggcccgt cggcttcatc ccggcggcct cggggtcgaa
      481 cgactacgcg caccacttcg ccgagtacat gggcgaggcg ttccagcggc aggtggaggt
      541 catccccatc tatcgcgccc gggacgcccg gcgcggcctg aactccgagc gtatccggga
      601 cgtcgccgcc gtctacatcg gcggcggcgt caccgatcac ctgctggacg ccctgcgcga
      661 caccccggcc accgaggcgc tggcgaccaa gatccgcgac ggcggcgtgg tcgtcgccat
      721 cgccgccgcc gcccaggcgg ccggccggct ggcgcgcagc atcttccgcg gcgacgccat
      781 cccgggcttc ggctggctgc ccgacgccgt ggtcgagccc aacttcgacc cggccaagga
      841 ccggcgcctg cgcaagctgt tggccgcagc cggcgctccg ggcgtcaact gggggctggg
      901 cattccggcc ggcaacgccg tgctcctcgg cccggaaggg gtcatggagg tggtgggcac
      961 cgccttccgc ctcggcgccc cggacgccgg gctggaagtg ttgggagacc ccgacttcga
     1021 gatcgccagc gacgaggatc tcgaagaaga gtagaatccg ctggattgaa aaagggaaaa
     1081 accttcaacc aaattttacg ccgaggcggg ttatgatccc cctggtgtga tgggagagat
     1141 acgagcatgg acacgactct tgcgatcctg aagcccgaca gtgtggaagc cggcagtgcc
     1201 ggcaagattc ttgcacatct tcaaaatgaa ggcttcacta ttcggggaat caagctgatg
     1261 cggctcacac aggcccaggc tcaggcgttc tatgaagtgc acaaggagcg tccgttctat
     1321 ggaagcctgg tggagttcat gaccagcggc ccggtgatgc cggtggccct cgagcgcgac
     1381 aacgcggtcg cccacctgcg gcaggtgatg ggggcgacgg acgtggccaa ggccgaggag
     1441 ggcaccgtcc gcaagctctt cggtacgtcc atcgagcgca acgcgatcca cggcagcgac
     1501 tcgccggaga acgcggcgat cgagctgtcc ttcttcttct cccgcgccga gctgcaggcg
     1561 gcgcgctagg accggacggg acgagccatg taccgcgaac cgcaagccgc tcccgagagc
     1621 cagctccccg aggagcggcg cggccgcacc atcggccgct tctgcctgac ctgctcgtcg
     1681 atctacccgc ttcaccgtct gcggcacgac ggcaagccga tgtacggcaa ggaccacgtc
     1741 gccgccccct gtgcccacga aggggacaac ttcgcgccgg gggaggggtg gtgggagccg
     1801 gcggtgctgg tgttgccgca ggcggctccg gcgccggagg gacagttcac gaacgcgccg
     1861 accaagggga cgcagccgtg aggggtggcg gggtttgcgg atgatcggat gagcgtcttc
     1921 tcccctcggc cggaggccgc cccggtgtac ggggcggcga tcgcccaggg ccgcctggcc
     1981 ctgtgggtga cgtggctccg gcaggggaga ggcaacgccc tggggatcgc gctcgccgcg
     2041 ggcctcctcg gggagctggt gagcggctgg ggggcgccgc ccctcgtccc cactctcccc
     2101 ttcacaccct tcgtctcctg cgccgccggc ctgctcctgg gctggcaggg cgtcgccgct
     2161 acctccgccg gacagttcct cgccatctgg atgacccgcc gcaacccgct ggaagcggcg
     2221 atcctggcgg tctgcctggc gctcctcggc gtcgccgccc acctggtctt ccgttccatc
     2281 ccgaagctgg gccgggggct gcccaacctc cggtcatatc tctggctgct cggcagtgcc
     2341 ctcttcggcg ggctggtcgc ggccggcatc ggcaacctcc tcctgccgac gcagcctata
     2401 gataccacct ggatctggaa ccgggcagcc ggcagcctgg tcagcgtgct cctcctggtg
     2461 ccgccgctgc tgctggtagc ggatcgccac ctccgcccct ggatcatgcc gatccccggc
     2521 gagatgccgg cgcggcgcgt ccgccgcttc ggcgaagacg tggcgagcga ggcggtgagc
     2581 gccgaggcga cggtgatgct ggagcggagc cgtccgcccg aggtcccgaa gggcgtgctg
     2641 gtcggggcgg ggctggtgct gggggtctcg gtcctggtgg tgccggtgat ctcgttgatc
     2701 ccggaagtag gcggctggct gcgcctcttc tatctgatcc cgatcctctg ggccgccctc
     2761 gccttcggct tgcgcggcgg cgtgctggcg gcgtcggcca gcggcttcgc gtacctcctg
     2821 gggatcgcgt ggatggcggc ggccgtggaa ccggcggagt cggcgcgggc tctgtggacc
     2881 gcctacgccg atctcttcgc cctgggtctc accggcgcct tcgtgggacg ctcgcgggag
     2941 cgcgagttcc atctgcggca ggagctggcc gacaccaacc gcctgctgcg ccgcgacatc
     3001 ctgcgggtgg cccaggcgct gacccaggcg gtgcaggcca aggacgagta caccgagggg
     3061 catctccacc gggtgagcga gtacgcggtg acggtgggtg agcggctggg gctgcgcggg
     3121 caggatctgg agatgctcca ctacgcgagc ctgctccacg acatcggcaa gatcggcatc
     3181 ccggagcagg tgctcaagaa gcccggccgg ctcgaggaag ccgaggccga ggcgatgcgg
     3241 cggcacccgg agatcggcgc ccggatgatc gagaacctgg acgtcctgag cggcgccgcg
     3301 ccgctcgtcc tccatcatca ggagcggtac gacggccgcc gcaacggcga gtacccgggc
     3361 tatcccggcg gcctcgccgg cgaccgaatc cccctgggcg cacgcatcat agccgtcgtg
     3421 gacgctttcg acgccatgac caccaaccgc ccctaccgcc gagcgctctc gccgcagcac
     3481 gccatcgccg tcctccagaa ggagaggggc cagcagttcg acccccgggt ggtggacgcc
     3541 ttcgtcagcc tcctcgccga acgtccgtgg gaagagtaga cggcggcctc gactgggagg
     3601 cggtggaccg ccatctggtg gaggtgcggg ggtgccgcaa gtgccctggc gtggtgccgc
     3661 cgccggtggc cgcccggccg tcgcagtggc cacgggtgat gctggtcggg caggcgcccg
     3721 ggcccaagga gcaggaggtg gggcgtccct tcgccttcac cgccggcacg cgcctcttct
     3781 cctggttcgc cgcgctgggg gtctccgagg aggagttccg ggagaaggtc tggatgacgg
     3841 cggtgatccg ctgcttcccc ggccgcgctc cccagggcgg cgaccgggtc cccgcgccgg
     3901 ccgagatcgc caactgctgg ccctacgtgg agcgggagct gcggctgatc cgtcccgcca
     3961 ctgtccttgc cgtgggacag ctcgccatcc gccacctcct ccccgacccc gcgccgctga
     4021 acgagagggt cggccgggtc ttcacctcct cccgggagga tctggagttc gacgtcatcc
     4081 cgctgccgca cccgtcaggc cgcagcactt ggctcgtccg tcccgagaac cgcgacctcc
     4141 tcgaccgcgc cctccggcac ctcgccgaga gccgggggtg gcgggagacg ttcggggagc
     4201 ggggaacctc tatacttgaa gtatgagtag aggtcaggat cccgcagcgt tggagcgcgc
     4261 ctctgcccgt agagcttggc cggtttgtgc cttccgactg ggtagcgagc cgagtgagga
     4321 cttgagcgcc aatacgaccg ccgtcgaaag gctggagatg atctggcccc tcaccgtgga
     4381 tgcctgggcg ctcgcgggcc ggatcatccc cgactatcct cgtcacgagg cccccatccg
     4441 ggtgatccac ggccacatcc gtaagcagtc ccgcgagggg gagcccgcct gaacagcgac
     4501 ttccaggaca tcttggccgc cttggtcgcg gagaacgcgg tctttctggt tgtgggggcc
     4561 catgccgtcg gtgtccatgg cgtacctcgg gcaaccggcg acctcgacat cctgattcgc
     4621 cctgacgccg agaacggcaa gcgtgtctgg cgtgcgctcg tacagtttgg tgctccggtc
     4681 gaggtgacgg gtctcacgcc gacagacctc gagaaaccgg acatggtcta ccagatcggc
     4741 ctgccaccgc ggcgaatcga tattctcacc gagatcagcg gcgtcgactt cgaagaggcg
     4801 tggagctccc gggttacttc gcgcgtgggg gatttggaga ttccgtttat cggaaaggat
     4861 gcgctgctgc ggaacaagag ggcgagcggc aggcctaagg acttggccga tatcgacatt
     4921 ctggagcggc gggagccttc ggagtagggg cccatggaca cgaccccagc ggagcaggcg
     4981 gttctcgccc ggttcaggga gcggtgccat atcgcgggcg tggtccaggt ggggtacggc
     5041 atgcgcgccg ccgccatccg cctggcccgg aacagccgcc cagagctcga cgtcgaccag
     5101 gcgctcaccg cgatggtgga gaagggctgg ctcaagcgca acgaggctgg gacctggtac
     5161 tacctgacgg cggacggggc cgaacaggtc aaggcgccgg cgccactctc agctctccag
     5221 gtcacctagt cctcatcacc ggccttctgg cccacctcca ggatggaagg cccgctccaa
     5281 cagggccaga ccgccgtcca ccagcagcgc caggacggcg gccggcacgg cgcccgacag
     5341 gatcatggcc gtgtcggcaa gctggagccc ggtgacgatg ggctccccca ggccgccggc
     5401 gccgatgaag gcggcgagcg tcgccgtgcc gaccgtgatc accgcggcgg tgcgcacccc
     5461 ggccatgatc accggcgccg ccagcggcag gcggatctgg cgcagcacct gcccgggcgt
     5521 catccccaac gcggtggccg cctccaccgc ccgcggatcg gactccctca cacctgtata
     5581 ggtgttccgg accatgggga agagcgaata gagccacagc gccgcgagag caggcacgaa
     5641 gcccacgccc aggaagggga ccatgaacgc cagcagggcg agggacggga tggtctgggt
     5701 gaggcccagg gcgcggatgg cgggctcggc gccgcggcgg tggcgctcca gccagagtcc
     5761 cagggggacg gccaccagga tccccaggag gagcgccgcg gcgcacaggc ccaggtgctc
     5821 cagagtccgg cgggcgaggc cgccccgctc gctccatgca taggaaagga agccgggccg
     5881 gcgcggcgcc gcctcctctc cggcggcggc gccctccagc aggccgagcg cgcgcagggc
     5941 gtcccgggcc acggcggcct cgctctcctt cccttcttga agacggacgt tgagccggcg
     6001 cattgtctcc tcgtccaggg cgccggcgag gagccccagg acggcgctca cctcggggtg
     6061 ctcgcgcaga gtggcgccgc ggaccagcgc ggcggcgtcg tagggtggga agaacccctt
     6121 gtcgtcctcc agtacctgga ggttatagag gacgagccgg ccgtccgtgg tgtagacgtc
     6181 gagggcatcg atcgcccggg agccggcggc ctggtacttc aaggcgtgct gcatcggctg
     6241 gaccgaggcg aagcgcagcc cgtagaccct ctgcagcccg agcaggccgt cctcgcggcc
     6301 gacgaactcg tggccgaagc cggcgcgcag acggggggcg gcgcgggcga ggtcggagat
     6361 cgtctcgatc ccctcgcgcc gggcgagctc ccgggggagc gccacctcga aggcgttctc
     6421 gaagcccaac ggggcgatcc accagaggtc ccagcgggcg aggaactccc ggcgcacccg
     6481 gttcagggtc tccgcgggtc ccccctcggg ctcctcgcca aggagggtca ccaggcccgt
     6541 gcccgtgtac tccgggtaga cgtcgatggc gccggtgcgg agggcctcga agcagacctg
     6601 ggtgccggcg aggccgagac ggcgttccac cctcaggcca gtgcggctct ccaggagctg
     6661 ggcgaacatc tcccccagga gacggctctc ctcgaagttc ttggagccga ccacgacggt
     6721 gccggtctcg gcggcaaggg tggggatggc gaggagcgag aagctgatga aaatcaggag
     6781 gcccctcatc ccgaccccct catctccagc agcgcccgca cgtaggggtc cgctgggctg
     6841 cggctcagct cctccggagg gccgagctgg aggagccggc cctggcgcat gacgccgata
     6901 cggtcggcca gacggaaggc ctctcccagg tcgtgggtca ccaggagggc ggtcttgcgc
     6961 aggcgcgcct cgaggtcgag gaactggcgg tggaggtcga gacgggtgag ggcgtcgagg
     7021 gcgccgaagg gctcatccag gaggatgacg tccgggttgg cggccagggc gcgggcgaag
     7081 gccacccgct ggcgctgccc tccggagagc tcggcggggt agcggcggcc gtactgcgcg
     7141 gggtcgagcc cgacgaggtc cagcatctcc cgtgtccgct gccggcggcg ctccgggtcc
     7201 cagccgagga ggcggggcac cagctccacg ttcctctcca cccgccagtg agggaggagg
     7261 ccgccgtcct gggggacgta gccgatgcgg cggcgcagcg tgatggggtc gagccgggcg
     7321 acgggctccc cctccacccg cacctcgccg gcggtgggct ccaccatccg gttgaagagg
     7381 cggaggagcg tcgtcttgcc cgagccgctc tcgcccacca gggccacggt ctcgcccggc
     7441 tggacggcca ggtcgacccc ggccagcgcc ggcgtgccgt ccggccaggt cttggcgacc
     7501 ccggtggctt ccagcgcggg tcccgcgggt agggaggaca cgcgcgtagt ctacaagggt
     7561 gggggagggg cgtggcggtg tagcatgacc aggccatgag acgagaaacc ctccgcttcc
     7621 caggcgcaca aggcgcggac ctcgccgccc ggctcgagag ccccgacgtc gatccgacgg
     7681 cgtacgcgct cttcgcccat tgcttcacct gctccaagga cctcaaggcg gcgggctgga
     7741 tcagccgtgc cctggtggag cgggggatcg ccgtgctgcg cttcgacttc accggcatcg
     7801 gagagagcga gggggacttc gccggcaccg acttctccgg caatgtcgcg gacctggtag
     7861 cggcggcgga cttcctgcgc acccgcgggg aggcgccgcg gctcctggtc ggccacagcc
     7921 tgggcggggc ggcggttctg gcggcggcgg tgcgcattcc ggagaccgcg gccgtggcca
     7981 ccatcgccgc gcccagcgac accgagcacc tgcgcgacaa gctcgtccgc ctcgccccgg
     8041 agctggagga gcgcggggag gccgaggtcg acctcggcgg cgagcgcccg ttccccatcc
     8101 gccggcagct cctggacgac ctggcggagg accatctgca ggaggccctc caccagctcc
     8161 acaagccgct cctcctcttc cactcgccgg tcgacaacgt ggtgggggtc gagcatgccc
     8221 accgcctgtt cgaggcggtc aagcacccga agagcttcat ctccctcggg ctcgccgacc
     8281 acctgctcac cgacgagcgg gacgcccggc acgtggggga gaccctggcc gcctgggccg
     8341 ggcgctacct ggaggggagc cagccggaga tcgaaaccct gcccgaggcg gggcggaccg
     8401 gggaggtggt ggtcaccgaa agaggacgct tcgcccagga catcgtcgcc cgccgccatc
     8461 gcctcaccgc cgacgagccc gagtccgtgg gcggcggcga caccggcccc accccctacg
     8521 acctgctcct cgccgccgtc ggggcgtgca cttccatgac cctcaggatc tacgccgacc
     8581 acaagaagct ccccctggaa ggggtgcggg tccggctccg gcactccaag gtccacgcca
     8641 ccgactgcgc cgtctgtgag accaaggagg gcaagctcga ccagatcgag cgggtcatcg
     8701 agttgctcgg gccgctcacc gacgagcagc gccagcggat gctcgagatc gccaaccggt
     8761 gcccggtcca ccgcaccctg gagtccgaga tccacgtgcc cacccggctg gagtgaccgg
     8821 cccggccggc ccgacctccc caggctccac ccccgtccaa ggcaggggac atccatccgg
     8881 ccccgccgcc gtcccggatg gaggattctt ccgtaagcca ctgaaaacaa agaggcgcag
     8941 tcctggactg ccagctggca cggatcgtgg actaggagta tgccggaggc ttcaaccgaa
     9001 tggacacgac gctcaccgcc actacgaccg agacgatcca gagggtccca gggaccgcgg
     9061 atctcccggg gcgggggcgc tagtcgatac gaacgaacag acagtctcct ctccctccgg
     9121 agccggggcg ggtcacacgc tctccgcccg tcccggccct cttttttccc ttttcagtcc
     9181 gttttcctgg cgtatggcgg gcaccccact ataatcagcc tcgtgagcga cggcgacgag
     9241 ttggagccgc gggtcgtacc gcggtccgag cacccgatat cccgcagaca gatcagcagc
     9301 aacgccttga aggtgatgta ccggctgcac aacgcgcggt acagggccta cctggtcggg
     9361 gggagcgtac gcgacctcat gctggggcgc gagccgaagg acttcgacgt gggcaccgac
     9421 gcccgcccca acgacgtccg ccggctgttc agcaactccc gcatcatcgg gcggcgtttc
     9481 cggctggtcc acgtcttctt ccatgacggc gagaccgtgg aggtctcgac gttccggcgc
     9541 aacccggagc cggaggagca gacctcggcg cccggggagc tgctgatcac cagcgacaac
     9601 accttcggca ccccgcgcga ggacgccttc cgccgggact tcacgatcaa cgccctgttc
     9661 tacgacatct ccagctattc ggtgatcgac tactgcggcg gcatcgagga cctggagcgg
     9721 cggctgatcc gggcgatcgg cgatccggat gtgcgcttcc aggaggaccc ggtgcgcatg
     9781 ctgcgcgcct gcgagttcgc cggacggctc ggcttcggca tcgagacgcg tacgcaggag
     9841 gccattcacc ggcaccgcaa ggagctggac aaggcctcgc cggcgcgggt caccgaggag
     9901 atcgcccaac tcctgcgttg cgggcgggcc ggcgccgcca tgcagtggat gctggagctc
     9961 ggcctcctcg aggtcctgct gcccgaggcc tacgccatgc tgacggcggg gcggcggggg
    10021 ttgggcgact tcggccagct gctgccggtc atcgaccgga tggtggccga agggcggcaa
    10081 ctgaccgacg gcgccctgct ggcggccatc ctcctgccca agatcctgct gcgccgggac
    10141 gacatcgaag ccgagcagag gcggccgatg agccgcgccg ccatcggcac gatgatcgag
    10201 gaggagatcc agcccttcct ctcccggctg accgtctcca acctgaaatc gacgcagatc
    10261 ctccaggcgc tcatcggctt ccaacgcctg tgcgagccga gctggacccc cggcgagcgc
    10321 gtccgcttct cccgcaagtc gttcttcgac aactcgttgc tgctcttcga gatgctggtg
    10381 gaggcgaccg gcgagggggg cgaggccctg gccgagtggc agtccgcggc gcgccggcgc
    10441 tccgctccgc cgtccgccga aggggcccgc cgcagccgtc cccggcgccg ccgccggcgc
    10501 caggggcagg ctttgtgaca tctttcacaa cctgaccgct ctggcgtagg ataggcgcgt
    10561 cccgtgcacc cgagaggaaa accgacccat gtcctggttc actgatctca cccgatccgc
    10621 cgtgggcaag aaggcggtga tggccgtcac cggcatcatc ctgttcggct tcgtgctcat
    10681 ccacatgatc ggcaacctga agctgtacgg cgggaaggac gccgagggcg tctaccacct
    10741 cgacagctac ggcggctggc tgcgggagat gggatccccc gcggtccccc atagcggcct
    10801 gctctggatc gcccggctcg tcctgctggc gtccgtcgtg ctccacatct ggtcctcgtg
    10861 ggcggtcacc cggatgagcc gcgacgcccg gccgcacgcc tacgccaagc gcaagccggt
    10921 gcgcatgacc tatgcctccc gcaccatgcg ctgggggggc gtgatcatcc tgctcttcgt
    10981 catctaccat ctgctccact tcacgaccgg caacgtccat ccggatttcc gggaaggggc
    11041 ggtcttccac aacgtggtga ccggcttcca gaacccactg gtgtcgctgt tctacatcgt
    11101 ggccaacgtc gccctgggct tccacctcta ccatgggctg tggagcctgt tccagtcgct
    11161 cggctggaac cacccgcggt tcaaccactg gcggcggggc ttcgcccacg ccttcgcctg
    11221 gatcgtcacc ctcggcaaca tatcgttccc gctggcggtg ctggccggca tcgtccgcct
    11281 ctaggagcca cccgtcatga agctcgagag caaggccccg acggggccac tggagacccg
    11341 gtggcaggag cacaaggacc acctgcggct ggtcaacccc aacaacaagc ggcggttcac
    11401 gatcctggtg ctgggctccg gcctggccgg tgcctcggcc gcggcgagcc tggccgagct
    11461 cggctacaac gtgaagtgct tctgcttcca ggacagcccg cgccgcgccc actcgatcgc
    11521 cgcccagggg gggatcaacg ccgccaagaa ctaccacaac gacggcgaca gcgtcttcag
    11581 gctgttctac gacacggtca agggcggtga cttccggtcg cgcgagagca acgtccatcg
    11641 tctggccgag ctgtcggtca gcatcatcga ccagtgcgtg gcgcaggggg tccccttcgc
    11701 ccgcgagtac ggcggcctgc tcgacaaccg ttccttcggc ggcgcgcagg tggcccgcac
    11761 cttctatgcc cgcggccaga ccggccagca gctcctgctc ggcgcctacc aggccctgag
    11821 caaggagatc gcctccggcc gggtcaagat gtacccacgc accgagatgc tggacctggt
    11881 ggtcgacaac ggccgcgccc tcggcatcgt cgcccgcgac atggtgacgg ggaagatcga
    11941 gagccacgcc ggccacgccg tggtcctggc gaccggcggc tacagcaacg tctactacct
    12001 gtcgaccaac gccaaggggt cgaacgtcac cgcctcctgg cgggcgcacc gccgcggcgc
    12061 gctgttcgcc aacccctgct atacccagat ccaccccacc tgcatcccgg cctcgggcga
    12121 ctaccagtcg aagctcaccc tgatgagcga gtcgctgcgc aacgacggcc gcatctgggt
    12181 gcccagggac ccgaacgacc agcgctcgcc ggaccagatc ccggacgccg accgcgacta
    12241 ctacctggaa cgcaagtacc cgagcttcgg caacctggtg ccgcgcgacg tcgccgcccg
    12301 caacgccaag atcatggtgg atgaggggcg cggcgtgggt ccgcagaaga acggcgtcta
    12361 cctcgacttc tcggcgtcca tccagcgcct gggcaaggcg gtcatcgccg agcgctacgg
    12421 caacctgttc gagatgtacg agaagatcac cggcgaagac ccctacgtgg tgccgatgcg
    12481 gatctatccg gctcctcact acaccatggg cgggctgtgg gtggactaca acctgatgag
    12541 caacatcgag gggctgtacg tcctcggcga ggcgaacttc tcggaccacg gcgccaaccg
    12601 cctgggggcg agcgcgctga tgcagggcct cgccgacggc tacttcgtca tcccctacac
    12661 catcggcgac tacctggcac cgcacctcgg ggagccgatc tcgaccgacg gcgccgcctt
    12721 ccgggcgacc gaggccgagg tgctcgaccg tatcaagcgc ctgacttcgg ccgccggtcg
    12781 ccgcagcccc gactcctacc accgcgagct cggccggatc gtctgggact actgcggcat
    12841 cgccaggaac gccggcggcc tgcgcaaggc gctgcagatg atcccggcgc tacgcgagga
    12901 cttctgggcc aacgtccgcg tcatgggcaa ggtggacgac ctcaaccagg acctcgagcg
    12961 ggcgctacgg gtggcggact acttcgagct cgccgagctg atgtgcctcg acgccctgga
    13021 ccgcgacgag tcgtgcggcg gccacctgcg ggaggagcac cagaccc
//
LOCUS       DASPJI010000804         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_853790, whole genome shotgun sequence.
ACCESSION   DASPJI010000804 DASPJI010000000
VERSION     DASPJI010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853790"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VEL74_22705"
     CDS             <1..221
                     /locus_tag="VEL74_22705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HYG65410.1"
                     /translation="VGARDLVREGRNGWTVPVEDVDALAGRMLWCARHPEEVRAMGLQ
                     CRRSAETATWPAYHRRLADLIVRLVAKP"
     gene            218..1507
                     /locus_tag="VEL74_22710"
     CDS             218..1507
                     /locus_tag="VEL74_22710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG65411.1"
                     /translation="MKILHVVPTYAPAWRHGGPIRAVHGLCKALAARGHEVTVFTTNV
                     DTGGAVPTSQPVSLDGVEVWYFPVRPPRRLYYSPDMGRALAGRVSGFDLVHLHSVFLW
                     PTAAAARAASRAGVPYLLAPRGMLVPDLLRRRGRLRKALWIRLVERRTLAGAAGLHVT
                     SGLEAGEAARLDLPLPRPFVIPNGVDPEPYLKDVPLSVPVRDALARAPFLLFLGRLSW
                     KKGLDRLIPALAHVPNTTLVLAGNDDEGYRPKLEQLAQSAGVAGRVLFTGLVDGADKA
                     ALLHRCAALVLPSYSENFGNAVLEAMAAGRPVAVTPEVGLAPIISSSGAGLVVDGDPV
                     HLGPALRDLLADPAGLDAMGQRAALEATTRFGWSAVAKSMESAYQAILTPHREGGRGP
                     VRPAQATGRWGGGPGGQHPKEPSTVEPGHDEAGRSRA"
     gene            1504..>2334
                     /locus_tag="VEL74_22715"
     CDS             1504..>2334
                     /locus_tag="VEL74_22715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65412.1"
                     /translation="MNLAFVVERPTQFEAPFYRYASQDPEHHLRVLFTDPRLSEPLFD
                     PELDRTVSWGFDLLAGYDHAVCPPSGRAAWLTRELRSRDLVIINGYTRAPYLQATAIA
                     HRLGIPTALRLDSVLWNGAPPRPHLKRLLFALALKPGYRLFLGTGTLTLDYLRFFGVS
                     EERTGLFPYAIDVDAFREGSTLSPEERAATRRRLGLPADARVLLSLAKLNPRESPWDL
                     LRAFTRNDGPDVATRWLVLAGDGPERPALEAFAQNQGSTQGSARVLFPGYIPYPELPA
                     L"
BASE COUNT          283 a          976 c          733 g          342 t
ORIGIN      
        1 tggtgggggc ccgtgacctg gtgcgggagg gccgcaacgg ctggaccgtg ccggtggagg
       61 acgtggacgc cctcgccggg cggatgctct ggtgcgcccg gcatcccgag gaggtccggg
      121 ccatgggcct ccagtgccgg cgctcggccg agacggcgac ctggcccgcc tatcaccggc
      181 ggctcgccga cctgatcgtc cgactggtgg cgaagccgtg aagatcctcc acgtcgtccc
      241 cacctacgcg ccggcctggc ggcacggcgg ccccatccgg gcggtccatg ggctctgcaa
      301 ggccctggcg gcccgcggcc acgaggtgac cgtcttcacc accaacgtgg acaccggcgg
      361 cgccgtgccc accagccagc cggtctcgct ggatggcgtc gaggtctggt acttcccggt
      421 ccggccgccc cgccgcctct actactcgcc ggacatgggg agagcgctgg ccgggcgcgt
      481 ctccggcttc gacctggtcc acctgcactc ggtcttcctc tggcccacgg ccgccgcggc
      541 ccgagccgcc agccgggcag gcgttcccta cctgctcgcc ccgcggggca tgctggtccc
      601 cgacctcctg cgccggcggg gccgattgcg caaagccctc tggatccgcc tggtcgagcg
      661 ccgcaccctt gccggcgccg cgggtctcca cgtcaccagc ggtctcgaag ccggagaggc
      721 ggcccgtctc gacctgccgc tcccccggcc cttcgtcatc ccgaacggcg tcgaccccga
      781 gccctatcta aaggatgtcc ctctctccgt tccggtccgg gacgccctcg cccgcgcgcc
      841 gttcctcctc ttcctgggac ggctgagctg gaagaaggga ctggaccgcc tgatcccagc
      901 cctcgcgcac gtcccgaaca cgaccctggt gctggcgggc aacgacgacg agggctaccg
      961 tcccaagctc gagcaactgg cccaatccgc cggcgtcgcc ggccgcgtgc tcttcaccgg
     1021 cctggtggac ggtgccgaca aggcggccct gctccatcgc tgcgcggccc tggtcctccc
     1081 ctcctactcg gagaacttcg gcaacgccgt cctggaggcg atggccgccg gccgcccggt
     1141 cgcggtgacc ccggaggtcg gcctcgctcc catcatctcc tcttccggag ccggcctggt
     1201 cgtcgacggc gaccccgtcc acctgggccc cgccctccgc gacctcctcg ccgaccccgc
     1261 cggcctggat gccatgggcc agcgcgccgc cctggaagcc accacccgtt tcggctggtc
     1321 cgccgtcgcc aagtccatgg aatccgccta tcaagccatc ctcaccccac accgggaggg
     1381 gggtcgtggc ccggttcgac cggcgcaggc gactggacgc tggggagggg ggcccggggg
     1441 ccagcacccg aaggagccga gcacggtcga accgggccac gacgaggcgg ggaggtcccg
     1501 cgcatgaacc tcgccttcgt ggtcgagcgc ccgacccagt tcgaggcgcc gttctatcgc
     1561 tacgcctccc aggacccgga gcaccacctc cgggtcctct tcaccgaccc gcgcctgtcc
     1621 gagcccctct tcgacccgga gctcgaccgc accgtctcgt ggggcttcga cctcctggcg
     1681 ggctacgacc acgccgtctg cccgccctcc ggccgcgccg cctggctcac ccgcgagctg
     1741 cggtcccgcg acctggtgat catcaacggc tacacccgtg ccccgtacct ccaggccacc
     1801 gccatcgcgc accgcctcgg catcccgacg gcgctgcgcc tcgactcggt cctctggaac
     1861 ggagccccgc cgcgcccgca cctcaagagg ctccttttcg ccctggccct caagccgggc
     1921 tatcgcctct tcctgggcac cggcacgctg accctggact atctccgctt cttcggcgtc
     1981 tccgaggagc ggaccggcct ctttccctac gcgatcgacg tcgacgcctt ccgcgagggc
     2041 tccaccctct cgcccgaaga gcgggccgcc acccgccggc gcctcggcct ccccgcggac
     2101 gcccgggtcc tcttgagcct cgccaagctc aacccgcggg agtccccctg ggacctgctc
     2161 cgcgccttca cccggaacga tggtcccgac gttgccaccc gctggctcgt cctcgccggc
     2221 gacggccccg agcgccccgc cctcgaagcc ttcgcccaaa accagggatc gacccaggga
     2281 tccgcccggg tcctcttccc gggctacatc ccctaccccg agctgccggc cctg
//
LOCUS       DASPJI010000805         5874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_109772, whole genome shotgun sequence.
ACCESSION   DASPJI010000805 DASPJI010000000
VERSION     DASPJI010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109772"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VEL74_22720"
     CDS             <1..476
                     /locus_tag="VEL74_22720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HYG65413.1"
                     /translation="QDFVKRLFKNVPVLDTGARGSTVTFVERGIGDVLLAWENEALLS
                     VRELGDKGEFQIVIPSLSILAEPPVTVVDKVANKRRTAALAKAYLEYLYTPQGQEIAA
                     RHYYRPRNAEVAAKYAQQFPQVQLITIDQVFGGWTQAQKAHFADGGILDKIYEGR"
     gene            477..1406
                     /gene="cysT"
                     /locus_tag="VEL74_22725"
     CDS             477..1406
                     /gene="cysT"
                     /locus_tag="VEL74_22725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HYG65414.1"
                     /translation="MVETADQKVPKRRSAELPAPLPVSLRSGGRQGRRGGSVLPGFGL
                     SLGFAVLYLSLIILIPLSTVFLKTATLTWEAFWQTVTSPRTLAAYRLSLWASFAAAAV
                     NLVFGVLLAWVLERYRFPGKRLIDAMVDLPFALPTAVAGIALATVYAQNGWLGEWLVP
                     MGINVAYTPLGVVVALTFIGLPFVVRTVQPVLADLDPEVEEAAASLGASRLQIVRKVI
                     LPELVPAAVTGFVLAFARALGEYGSVIFIAGNMPMKSEITPLLIMIKLEQFDYAGATA
                     VAMVFLMASFSLLLVINFLAWRRNRNLRAAETE"
     gene            1418..2281
                     /gene="cysW"
                     /locus_tag="VEL74_22730"
     CDS             1418..2281
                     /gene="cysW"
                     /locus_tag="VEL74_22730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HYG65415.1"
                     /translation="MTAGNRLVRGGGGHAERTGALTEPRLVRWLLLGITFAFLGFFLF
                     VPLLLVFVKALENGVDAYWAAVNEPIALAAVRRTLLTAVIAVPLNLLFGLAAAWAIAK
                     FRFPGKQLLITLIDLPFSVSPVISGMVFVLLFGAQGYLGHWLQANEVKIIFAVPGIVL
                     ATIFVTSPLVARELIPLMQSQGNDEEEAALTLGASGWQMFRRVSLPKIRWGLLYGVVL
                     TNARAMGEFGAVSVVSGHIRGVTTTLPLHVEILYNEYQFSAAFAAASLLTLLALITLG
                     IKSAIEWRTAR"
     gene            2357..2917
                     /locus_tag="VEL74_22735"
     CDS             2357..2917
                     /locus_tag="VEL74_22735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HYG65416.1"
                     /translation="MNRSSTPVEKYFEKFLWSSRYLIIVAVLASLAGAVAMLYVATVD
                     AVELISHLPDYHRAADPDQRMALRSGVVTHIVEMVDGYLLASIMLIFSLGLYELFISG
                     IDPAVGSENKSRVLVIENLDDLKDRLAKVVLLILIVKFFEQVLSLTFETPLELLYLAV
                     GIALVAGALFLSHGKSHGSHEKAARG"
     gene            2935..4986
                     /locus_tag="VEL74_22740"
     CDS             2935..4986
                     /locus_tag="VEL74_22740"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG65417.1"
                     /translation="MRRPGFRTLLVGLNAGLVLLAILALVLTGSELLERLADEQALAR
                     VDLAGVAARQAVERSAGDVRTSARLLAERPTVDRLLRERDAAQLAAFLERFRRTSGLS
                     GCALLIDGRPLVAAGPPLPWEALAGSRGQPVTVGGGLALAAASPLPALPGAVAVTAAA
                     LDEAFVRRAAGQVGLPVDVIAAPEALARTDDPRWVLRSDAVETGRPARGRLEEAGLYL
                     AVRPVQVAGEVVALVEASLSTAGVQASVAALERQLLLLSAVVAGVAILSGLVLGRRLA
                     RPVRSLTVAASRIGGGDLSTPVPRAAGPELGTLATTMEEMRRHLLRLTSELRRQQAEG
                     EAILTGIVEGVLSVDRDRRIRYLNPQAAAILKIDPQETLGRFCGDVLNPAGPGGVRPC
                     HEHCPILHARFRGGTSSVEHLQLADGTRRSVVITSAPPTEDRQVLVLRDETDLEAARR
                     LRDAVLANISHEFKTPLSAQLASIELLRERLEETEDKESGALVQSLERGSLRLTQLID
                     NLLESVRIEAGEASVRRRPVALDEVVEEAVTLTAPLLAQRGQTPEVDLPYPLPEVRGD
                     APRLIQVFVNLLANANKYAPEGSVIRVGGAVEQGAVALWVEDEGPGLPKGEEGGRSLF
                     ARFVRSAGDDEPEQSGMGLGLWIVQSIVERHGGTVDAEPRPVGTRMVVRLPRQISKEV
                     A"
     gene            4983..5669
                     /locus_tag="VEL74_22745"
     CDS             4983..5669
                     /locus_tag="VEL74_22745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017578235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYG65418.1"
                     /translation="MKILVADDDLELRGLIAYALRQAGYLVVEAGDGAAALELFDREE
                     PVLVILDVNMPRLSGLQVCRRLREISKAPVMMLTVRSGEEDQVQALDLGADDYLTKPF
                     SPRSLLARVRALLRRAGAEKPAPLAAGDFSLDVETQAVSVAGAAPVRLTRLEFRLLQL
                     LLANAGHTLPAERLLSHVWGSRGGGDRQLLKQLVHRLRQKIEADAAEPRYLVTVSGVG
                     YALRPAGDGE"
BASE COUNT          764 a         2116 c         2062 g          932 t
ORIGIN      
        1 tccaggactt cgtcaagcgt ctgttcaaga acgtgcccgt gctcgacaca ggcgcgcgcg
       61 gctccaccgt cacctttgtc gagcgcggca tcggcgacgt gctcctcgcc tgggagaacg
      121 aggcgctgct gtcggtgcgc gagctcgggg acaagggcga gttccagatc gtcatcccgt
      181 cgctcagcat cctggccgag ccgccggtga ccgtggtcga caaggtggcc aacaagcgca
      241 ggacggcggc gctggccaag gcgtatctcg agtacctgta caccccccag ggccaggaga
      301 tcgccgcccg ccactactac cggccgcgca acgccgaggt ggcggccaag tacgcccagc
      361 agttcccgca ggtgcagctc atcaccatcg atcaggtctt cggcggctgg acgcaggcgc
      421 agaaggcgca cttcgccgac ggcgggatct tggacaagat ctacgagggc cggtaaatgg
      481 tcgagacagc ggatcagaag gtccccaaga ggcggagcgc ggagctcccc gcgcccctgc
      541 cggtctccct gcggtcgggt ggccgccagg gccggcgcgg cggcagcgtc ctgcccggtt
      601 tcgggctgtc gctgggcttc gcggtgctct atctctcgct gatcatcctg atcccgctgt
      661 ccacggtctt cctcaagacc gccaccctca cctgggaggc tttctggcag accgtgacgt
      721 cgccgcggac gctggcggcc taccggctga gcctgtgggc ctccttcgcc gcggcggcgg
      781 tcaacctcgt cttcggcgtg ctcctcgcct gggtgctgga gcgctaccgg ttccccggca
      841 agcgcctgat cgacgccatg gtggacctcc ccttcgccct gcccacggcc gtcgccggca
      901 tcgccctggc cacggtctac gcccagaacg gctggctcgg tgaatggctg gtgccgatgg
      961 gcatcaacgt cgcctatacg ccgctcggcg tggtggtggc cctcaccttc atcggcctcc
     1021 ccttcgtcgt gcggacggtc cagccggtgc tggccgacct cgacccggag gtggaggagg
     1081 cggcggccag cctcggcgcc agccgccttc agatcgtccg caaggtcatc ctgcccgagc
     1141 tcgtgccggc ggcggtcacc ggcttcgtcc tggccttcgc ccgcgccttg ggcgagtacg
     1201 gctccgtgat cttcatcgcc ggcaacatgc cgatgaagtc cgagatcacc ccgctcctca
     1261 tcatgatcaa gctcgagcag ttcgactacg ccggggccac ggctgtcgcc atggtctttc
     1321 tgatggcatc gttctcgctg ctcctcgtca tcaacttcct ggcctggcgc cggaaccgca
     1381 acctgcgcgc cgctgaaacg gagtagacgt catggccatg acagcaggca atcgattggt
     1441 ccgtggaggc ggcgggcacg ccgagaggac cggcgcgctc accgagccgc ggctcgtccg
     1501 ctggctgctg ctggggatca ccttcgcctt cctcggcttc ttcctcttcg tgccgctgct
     1561 cctggtcttc gtcaaggcgc tggagaatgg cgtggacgcc tactgggcgg cggtgaacga
     1621 gcccatcgcc ctggcggccg tgcgtcggac gctgctgacc gccgtgatcg cggtgccgct
     1681 caacctgctc ttcggcctcg cggcggcttg ggcgatcgcc aaattccgtt ttcccggcaa
     1741 gcagctcctg atcaccctga tcgacctgcc gttctccgtc tcgccggtga tctcgggcat
     1801 ggtcttcgtg ctgctcttcg gcgcccaggg ctacctcggc cactggctcc aggcgaacga
     1861 agtcaagatc atcttcgccg tgcccggcat cgtgctggcg accatcttcg tcacctcgcc
     1921 gctggtggcg cgggagctga tcccgctgat gcagtcccag ggcaacgacg aggaggaggc
     1981 ggcgctcacc ttgggggcgt cgggatggca gatgttccgg cgggtaagcc tgccgaagat
     2041 ccgctggggc ctgctctacg gcgtggtgct caccaacgcc cgggcgatgg gcgagttcgg
     2101 cgccgtctcg gtggtctcgg gacacatccg gggcgtcacc acgacgctgc cgctgcacgt
     2161 cgagatcctc tacaacgagt accagttctc ggccgccttc gccgcggcct cgctgctgac
     2221 cttgctcgcc ctgatcaccc tcggcatcaa gagcgccatc gagtggagga ccgcgcgctg
     2281 accggagcgg atacaatccc ctcttcgaat cccggaacgg agtgcacggt cacggggacg
     2341 caggggggga ttgcgcatga acaggtcgtc gacgccggtc gagaaatact tcgagaagtt
     2401 cctctggagc agccgctatc tgatcatcgt ggcggtgctg gcgagcctcg ccggagcggt
     2461 cgccatgctg tacgtcgcca cggtggacgc ggtggagctc atctcgcatc tgcccgacta
     2521 ccatcgggcc gccgacccgg accagcggat ggcgctgcgc tcgggggtcg tcacccacat
     2581 cgtggagatg gtggacggtt acctgctggc gtccatcatg ctgatcttct ccctggggct
     2641 ctacgagctg ttcatcagcg gcatcgaccc ggcggtgggc tcggagaaca agtcccgggt
     2701 gctggtgatc gagaacctgg acgacctcaa ggaccggctg gccaaggtgg tgctgctgat
     2761 cctgatcgtc aagttcttcg aacaggtgct cagcctcacc ttcgagaccc ctctcgagct
     2821 cctctacctg gcggtgggga tcgccctggt ggccggagcg ctcttcctgt cgcacggcaa
     2881 gagccacggg agccacgaga aggcagcgcg cgggtaggcg tacacttccc ccgcatgcgg
     2941 cggcccggtt tccgcaccct cctcgtcggc ctgaacgccg gcctcgtcct gctggcgatc
     3001 ctggcgctgg tcctgaccgg ctcggagctc ctggagcgct tggcggacga gcaggcgctg
     3061 gcgcgggtcg atctcgccgg cgtggcggcc cgccaagcgg tggagcgctc cgccggggac
     3121 gtgcgcacct cggcccgtct cctcgccgag cgcccgacgg tggaccgcct cctccgcgag
     3181 cgggacgccg cgcagctcgc cgccttcctg gagcgcttcc ggcgcaccag cggcctgtcg
     3241 ggctgcgccc tcctgatcga tggccggccg ctcgtggccg cggggccgcc gctcccctgg
     3301 gaagccctgg ccggcagccg cggccagccg gtcaccgtcg gcggcggcct cgccctcgcc
     3361 gcggcgtccc ccctccccgc gcttccgggc gccgtggccg tgacggcggc ggctctcgac
     3421 gaggcgttcg tccgtcgcgc cgccggacag gtcggcctgc ccgtggacgt gatcgccgcg
     3481 ccggaggccc tggcgcggac ggacgatccg cgttgggtcc tgcgcagcga cgcggtggag
     3541 accggacggc cggccagggg ccggctggag gaggccggcc tctacctggc ggtccggccc
     3601 gtccaggtgg ccggcgaggt ggtggctctg gtcgaggcct ccctctccac ggccggggtg
     3661 caggcgtcgg tggccgccct ggagcgccag ctgctgctcc tctccgccgt ggtggccgga
     3721 gtggcgatcc tctccggcct cgtgctcggc cgccgtctcg cgcggccggt ccgttcgctc
     3781 accgtggccg cctcgcgcat cggcggcggc gacctgtcga cgccggtgcc acgggctgcg
     3841 gggcccgagt tgggcaccct cgccacgacc atggaggaga tgcgccggca cctgctgcgc
     3901 ctcacctccg agctgcgccg ccagcaggcc gagggggagg cgatcctcac cggcatcgtc
     3961 gagggtgtcc tgagcgtcga ccgcgaccgc cggatccgct acctcaaccc gcaggccgcg
     4021 gcgatcctga agatcgatcc gcaggagacg ctgggccggt tctgcggcga cgtcctgaac
     4081 ccggccggac cgggcggcgt gcggccctgc cacgagcact gcccgatcct ccacgcccgc
     4141 ttccgcggcg gcaccagcag cgtcgagcac ctgcaactcg cggacggcac gcggcgctcc
     4201 gtggtgatca ccagcgcgcc acccaccgag gaccgccagg tcctggtgct gcgcgacgag
     4261 accgacctgg aggcggcgcg gcggctgcgc gacgcggtgc tggccaacat ctcccacgag
     4321 ttcaagacgc cgctctcggc gcagctcgcc tccatcgagc tcctgcgcga gcgcctggag
     4381 gagacggagg acaaggagtc cggcgccctg gtacagtcgc tggaacgggg gagcctgcgc
     4441 ctcacccagc tcatcgacaa cctcctggag agcgtccgca tcgaggccgg cgaggcgtcg
     4501 gtccgccgcc ggcccgtggc cctggacgag gtggtggagg aggcggtcac cctgaccgcg
     4561 ccgctcctcg cccagcgcgg ccagaccccg gaggtcgatc tgccctatcc ccttcccgag
     4621 gtgcgggggg acgcgccgcg gctgatccag gtcttcgtca acctcctcgc caacgccaac
     4681 aagtacgcgc ccgagggctc ggtgatccgg gtcggcggtg cggtggagca gggggcggtg
     4741 gccctgtggg tggaggacga ggggccgggc ctgccgaagg gggaggaggg ggggcggtcg
     4801 ctcttcgccc gcttcgtccg ctcggccggc gacgacgagc cggagcagag cggcatgggg
     4861 ctggggctgt ggatcgtgca atcgatcgtc gagcggcacg gcgggacggt cgacgcggag
     4921 ccccgccccg tggggacccg gatggtcgtg cgtctgcccc ggcagatatc caaggaggtg
     4981 gcgtgaagat cctggtggcg gacgacgacc tggagctgcg tgggctgatc gcctatgcgc
     5041 tacggcaggc cggctatctg gtagtggagg ccggagacgg cgccgcggcc ctggagctct
     5101 tcgaccgtga ggagccggtc ctggtgatcc tggacgtcaa catgccgcgc ctctcgggcc
     5161 tccaggtctg ccgccgcctg cgcgagatct cgaaggcgcc ggtgatgatg ctcaccgtgc
     5221 gctccggcga ggaggaccag gtccaggcgc tcgacctggg ggccgacgac tacctcacca
     5281 aacccttcag cccgcgctcg ctgctcgccc gggtacgggc cctgctgcgc cgcgccggcg
     5341 cggagaagcc cgcacccctg gccgccggcg acttctcgct cgacgtggag acccaggcgg
     5401 tgagcgtcgc gggcgcggcg ccggtccgcc tcacccgcct ggagttccgg ctcctgcagc
     5461 tcctcctcgc caacgccggc cacaccctgc cggccgagcg cctcctctcc cacgtctggg
     5521 gatcgcgggg aggcggagac cgccagctcc tcaagcagct cgtccaccgt ctccgccaga
     5581 agatcgaggc cgacgccgca gagccccgct atctggtgac cgtgagcggc gtcggctatg
     5641 ccttgcggcc agcgggggac ggggagtaag gcatccgccc ggcgggcggg ctcatccccg
     5701 cggaccggcg cttcatcctt ccggccgagc gtgatgcccg gccggcggga tggttcatcg
     5761 gcccggcggg cgggctcatc cctgcggacc ggcgcttcat cctgccggcc cggcgtgatg
     5821 cccggcggcg ggacggttca tcggtccggc gggcgggctc atccctgcgg accg
//
LOCUS       DASPJI010000806         4870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_109887, whole genome shotgun sequence.
ACCESSION   DASPJI010000806 DASPJI010000000
VERSION     DASPJI010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109887"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VEL74_22750"
     CDS             <1..1149
                     /locus_tag="VEL74_22750"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HYG65419.1"
                     /translation="HRRGSFFIAGTGEPDPAGPWPEPAGRPIELPEPMAPKPWNVPSD
                     FDTPMIGESEGLKLLESLGRELASELPGARLLRAVLEDGSSEGEVISSRGLRARLRHR
                     VASLYLEAAGPAPSGRPAATATLYLYAREARRFHPPALARRLADRLAVAAAGAPPPGP
                     DAAECLLAPPVMARLLAGLLPLLVGPQSTAYLSGRRDRRGKIGSDLLTLLDNGRLQGG
                     AFESPVDGEGVPCSEVVLVEEGSYRQPLLAWWQESAAPGTPSGCSRRASWRDIPAPGP
                     THLYLKPDARTPVAALLGAVERGYYLLDATGGARFDFGADRFALPVCGFAVAGGRAQA
                     PVAGAWLCGGIGTLLHGIRAVGRDLAFHPLDGMIGTPTVLVTGLELRG"
     gene            1234..1884
                     /locus_tag="VEL74_22755"
     CDS             1234..1884
                     /locus_tag="VEL74_22755"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HYG65420.1"
                     /translation="MKLDALIRWFLPKEERFHELLARDTANLLRASRLFSEFVYATDI
                     ETRRIKAAELKDREHEGDRITRQVFEALYSSFLTPLDREDIRSIATDLDDILDYLEGV
                     AQHLVLFELSDAPEGLKQFADILVEMAVEIDRLTQLIWDLGNGQKIHAAIVRISDLEN
                     RADAVYNTLITELFRGKGRDPIEILKWKEVYEGLENACDQCKDFTHIIGNVVLKNS"
     gene            1895..2890
                     /locus_tag="VEL74_22760"
     CDS             1895..2890
                     /locus_tag="VEL74_22760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HYG65421.1"
                     /translation="MTTGFALLVFIILVALTFDFLNGFHDAANSIATVVTTRVLSPSQ
                     AVIWAAFFNFIAAFVMGTHVAKTIGKGMIDLSVVTQEVILAGLLGAIAWNLITWYYGI
                     PVSSSHALIGGYAGAAVAKAGFTALLLGGWVKTLMFIVLAPMIGMLLGFFLMIAVIWS
                     VRRWRPAKVDRLFRKLQLVSAGLYSLGHGGNDAQKTMGIITGLLVASGYLAEFEVPLW
                     VVLISHAAIALGTMFGGWRIVRTMGSRITKLQPVGGFCAETAGAITLVGATLGGIPVS
                     TTHTITGAIVGVGATRRISAVKWDVAGRIVWAWVLTIPIAAAISAVSYYLIAPLI"
     gene            complement(2894..4594)
                     /locus_tag="VEL74_22765"
     CDS             complement(2894..4594)
                     /locus_tag="VEL74_22765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG65422.1"
                     /translation="MRRFALTLGFALLAGLPAAAQSPQPAQAPQPPFGEEIDVRVVNV
                     EAVVTDRQGNRVPGLQPGDFRLLVDGKEVPIEYFTEVRGGQAVTPEAPGAAGNEGRTP
                     VVPGLASLAPGSPVGTSYLLFIDDYFSLAPRRNEVLRALQDDLGRLGPEDRMAVVAYD
                     GGELKMLTTWSNSSREINRALQEAIARPARGIDRLTDLRTFESSRRFSAGSVPGAGMP
                     RAATSGILSFEERNYAQELARQVADVSGAAASALRGFAAPPGRKVMLLLSGGWPLRPV
                     DYVVNSFERTILTREAPEGEALLRPLIRTANLLGYTIYPVDVPGVEAVSADATESGGP
                     SLGTSNVREQEIEDSLELVAARTGGRPLVNGLRAAALEQAAADTRSYYWLGFTPTWQR
                     NDQPHAVKVEVRRDDLRGLAVRSRDSFFDVSRRAEVTMMVESAMLFGSAPGFATMPVQ
                     VGTPKRAGRGQVEVPVTVSIPADAFTPLPAEGKFAAQLELRIASVDERGGRSAIPVIP
                     LSLAGDKPPAAGSSLTYQTSLRLRDALQHLVVALYDPVSGKITTGEVDFIPEKARRKR
                     "
     gene            complement(4674..>4870)
                     /locus_tag="VEL74_22770"
     CDS             complement(4674..>4870)
                     /locus_tag="VEL74_22770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65423.1"
                     /translation="TLRNDHVVRRLDRAFTLPGAAGSKGSGELVLGLDKRWKRPNLRI
                     VAFLQDPRTLEIHGAAVREL"
BASE COUNT          663 a         1803 c         1697 g          707 t
ORIGIN      
        1 caccgccgcg gctccttctt catcgccggc accggcgagc ccgacccggc cggcccctgg
       61 cccgagccgg cggggcggcc catcgagctg cccgagccga tggcgcccaa gccctggaac
      121 gtgccgtccg acttcgacac gccgatgatc ggcgagagcg aggggctgaa gctgctcgag
      181 agcctggggc gcgagctggc ctccgagctc cccggcgccc ggctgctgcg cgccgtcctc
      241 gaggacggtt cgagcgaagg ggaggtgatc agcagccgcg gcctgcgcgc ccgcctgcgc
      301 caccgggtgg cgagcctgta cctggaggcc gccggccccg ccccctcggg acggcccgcc
      361 gccaccgcca ccctctatct ctatgcccgg gaggcgcggc gcttccaccc cccggccctc
      421 gcccgccgcc tcgccgaccg cctggcggtg gcggccgccg gcgcgccgcc ccccgggccg
      481 gacgcggccg agtgcctcct cgctcccccg gtgatggccc gcctcctcgc cggcctgctg
      541 ccgctgctcg tcggccctca gtccaccgcc tacctctccg gccggcggga ccgccggggg
      601 aagatcggca gcgacctgct gacgctgctc gacaacggcc ggctccaggg gggcgccttc
      661 gagtcgccgg tggacggcga aggggtgcct tgcagcgagg tcgtgctggt cgaggagggg
      721 agctatcgcc agcccctgct cgcctggtgg caggagagcg ccgcccccgg caccccgtcc
      781 ggctgcagcc gccgggcgag ctggcgcgac atcccggcac cggggccgac ccacctctat
      841 ttgaagccgg acgcccgcac gccggtggcc gccctgctcg gcgcggtgga acgcggctac
      901 tacctgctgg acgccaccgg cggcgcccgc ttcgacttcg gcgccgaccg cttcgccctg
      961 ccggtctgcg gcttcgccgt cgccggcggc cgcgcccagg cgccggtggc cggcgcctgg
     1021 ctctgcggcg gcatcggcac cctcctgcac ggcatccgcg cggtagggcg cgacctcgcc
     1081 ttccacccac tggacggcat gatcggcacc cccaccgtcc tggtcaccgg tctcgagctc
     1141 cgcggttgac cttccggggg gccatggtac gatgcgcccg ccgccgtgac acggaggtca
     1201 cctccgcgtc acatcccggc ggggagcaag cccatgaaac tcgatgcctt gatccggtgg
     1261 ttcctgccga aggaggagcg cttccacgag ctgctggcgc gtgacaccgc caacctgctc
     1321 cgcgcctccc gtctgttctc cgagttcgtc tacgccaccg acatcgagac gcggcggatc
     1381 aaggcggccg agctcaagga ccgcgagcac gagggggacc ggatcacccg gcaggtgttc
     1441 gaggccctct actccagctt cctgacgccg ctcgaccgcg aggacatccg ctcgatcgcc
     1501 accgacctgg acgacatcct cgactatctg gagggggtgg cccagcacct ggtgctcttc
     1561 gagctgtccg acgcgccgga ggggctcaag cagttcgccg acatcctggt ggagatggcg
     1621 gtggagatcg accgcctgac ccagctcatc tgggacctcg gcaacggcca gaagatccac
     1681 gcggcgatcg tccgcatctc ggacctcgag aaccgcgccg acgcggtcta caacacgctg
     1741 atcaccgagc tgttccgcgg caaggggcgg gacccgatcg agatcctcaa gtggaaagag
     1801 gtgtacgagg gtctcgagaa cgcctgcgat cagtgcaagg acttcaccca catcatcggc
     1861 aacgtcgtcc tcaagaactc ctgagacacc gcccttgacc accggcttcg cgctcctcgt
     1921 cttcatcatc ctcgtcgcgc tgacgttcga tttcctgaac ggcttccacg acgccgcgaa
     1981 ctccatcgcc acggtcgtca cgacgcgggt gctgtcgccc agccaggcgg tcatctgggc
     2041 ggcgttcttc aacttcatcg ccgccttcgt catgggcacg cacgtggcga agaccatcgg
     2101 caaggggatg atcgacctgt cggtggtgac gcaggaggtg atcctcgccg gcctgctcgg
     2161 ggcgatcgcc tggaacctga tcacctggta ctacggcatc ccggtctcgt cgtcgcacgc
     2221 gctgatcggc ggctacgccg gggcggcggt ggcgaaggcc gggttcaccg cgctgctgct
     2281 cggcggctgg gtcaagacgc tgatgttcat cgtcctggcg ccgatgatcg gcatgctgct
     2341 cggcttcttc ctgatgatcg cggtgatctg gagcgtgcgc cgctggcgcc cggccaaggt
     2401 ggaccgcctg ttccgcaagc tgcagctcgt ctcggccggc ctctacagcc tcggccacgg
     2461 cggcaacgac gcccagaaga cgatggggat catcaccggc ctgctggtcg cctcgggcta
     2521 cctcgccgag ttcgaggtgc cgctctgggt ggtgctgatc agccacgcgg ccatcgccct
     2581 gggcaccatg ttcggcggct ggcggatcgt gcgcacgatg gggagccgca tcaccaagct
     2641 ccagccggtg ggcggcttct gcgccgagac cgccggtgcc atcaccctgg tgggggcgac
     2701 gctggggggc atcccggtgt cgacgacgca caccatcacc ggcgccatcg tcggcgtcgg
     2761 cgccacccgg cgcatctcgg cggtcaagtg ggacgtcgcc ggccgcatcg tctgggcctg
     2821 ggtcctcacc atccccatcg ccgccgccat ctccgccgtc agctactatc tgatcgcgcc
     2881 gctgatctga cccctaccgt ttccggcggg ccttctccgg aatgaagtcc acctcgccgg
     2941 tggtgatctt gccgctcacc gggtcgtaga gggcgacgac caggtgctgc agggcgtcgc
     3001 gcaggcgcag gctggtctgg taggtgaggg aggagccggc cgccggcggc ttgtcgccgg
     3061 cgaggctcag ggggatcacc gggatggccg accggccgcc gcgctcgtcc acggaggcga
     3121 tccgcagctc gagctgcgcg gcgaacttgc cctccgccgg caagggggtg aaggcgtccg
     3181 ccgggatcga gacggtgacc ggcacctcca cctgcccccg cccggcgcgc ttcggcgtcc
     3241 cgacctggac cggcatggtg gcgaagcccg gggcgctgcc aaagagcatc gcgctctcca
     3301 ccatcatcgt gacctcggcc cgccgcgaga cgtcgaagaa gctgtcccgc gagcgcaccg
     3361 cgagccccct gagatcgtct cgccgcacct ccaccttgac ggcgtggggc tggtcgttgc
     3421 gctgccaggt cggggtgaag cccagccaat agtaggagcg ggtgtccgcc gcggcctgct
     3481 ccagcgccgc ggcgcgcagc ccgttgacca gcggccggcc gccggtccgc gcggcgacca
     3541 gctccaggga gtcctcgatc tcctgctcgc gcacgttgga ggtccccagg gagggcccgc
     3601 cggactccgt ggcgtccgcc gacaccgcct cgacgcccgg cacgtccacc ggatagatgg
     3661 tgtagcccag gaggttggcg gtgcggatca gcggccggag cagcgcctcc ccctcggggg
     3721 cctcgcgggt gaggatggtg cgctcgaagc tgttcaccac gtagtccacc gggcgcaggg
     3781 gccagccgcc ggagagcagg agcatgacct tccggcccgg cggcgccgcg aacccgcgca
     3841 gcgcgctggc ggcggccccc gagacgtccg ccacctggcg ggcgagctcc tgggcatagt
     3901 tgcgctcctc gaaggagagg atgcccgagg tcgccgcccg cggcatgccc gcccccggga
     3961 ccgaccccgc cgagaagcgg cgcgagctct cgaaggtgcg caggtcggtg agccggtcga
     4021 tgccgcgggc gggacgggcg atcgcctcct ggagcgcccg gttgatctcg cgcgaggaat
     4081 tcgaccaggt ggtcagcatc ttcagctcgc cgccgtcata ggcgacgacg gccatccggt
     4141 cctccggccc gagccgcccg aggtcgtcct gcagggcgcg cagcacctcg ttgcggcgcg
     4201 gcgcgaggga gaagtagtcg tcgatgaaga gcaggtagct ggtcccgacc gggctcccgg
     4261 gggcgaggct cgccaggccg ggcaccaccg gcgtccgccc ctcgtttccc gcagctccag
     4321 gagcttccgg ggtgaccgcc tgcccgccgc gcacctcggt gaagtactcg atggggacct
     4381 ccttgccgtc caccagcagc cggaaatcgc cgggctggag gcccggcacc cgattgccct
     4441 gacggtcggt gaccaccgcc tcgacgttga ccacccggac gtcgatctcc tcgccgaagg
     4501 ggggctgagg ggcctgggcg ggctgggggc tctgggcggc ggcgggaagc ccggcgagca
     4561 gggcgaatcc gagggttagg gcaaagcggc gcatggcgat ctctcctggg agcgcgaagg
     4621 gcaggctctc ccgagagtgt aagaccaggc agccgcctct ggaattcctc ctcctacagc
     4681 tcccgcaccg cggcgccgtg gatctccagc gtgcgcgggt cctggaggaa ggcgacgatc
     4741 cgcaggttcg gcctcttcca gcgcttgtcg aggccgagga ccagctcacc cgagcccttc
     4801 gagccggcgg cgcccggcag ggtgaaggcc ctgtcgagcc gccgcaccac gtgatcgttg
     4861 cgcagcgtct
//
LOCUS       DASPJI010000807         4778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_452301, whole genome shotgun sequence.
ACCESSION   DASPJI010000807 DASPJI010000000
VERSION     DASPJI010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452301"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VEL74_22775"
     CDS             complement(<1..973)
                     /locus_tag="VEL74_22775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG65424.1"
                     /translation="MNWLSQVMAVTGLNLRTIGQRLGASAAAVFGVAGVVAVFVAVLS
                     IAEGFRQTVTASGSEDTALVLRSGSDSEMMSGLERETTRLIADAPGVARSAGGPMASA
                     ELFVIVDLPKRTTGTDANVPLRGVQSAAFDVRDEVEIVEGRTFRPGSNEIIAGRAAAA
                     EFAGLEVGARHRWGESEWQVVGIFEAGGGLAESEIWCDAGVLQPAYRRGSTFQAVYAK
                     LDSPASFDRFKDTLTTDPRLDVKVIRETEYYADQSRILYSIITGLGTIIALLMGVGAV
                     FGALNTMYTAVSSRTREIATLRALGFGGGPVVLSVLAESLFLSLLGGG"
     gene            complement(978..2132)
                     /locus_tag="VEL74_22780"
     CDS             complement(978..2132)
                     /locus_tag="VEL74_22780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYG65425.1"
                     /translation="MKYLPLLWSNLKRKKVRTLFTLLSILVAFLLFGYLAAIRMAFSM
                     GVDVVGVDRLLVVHKVSIIQPIPISYKDRIAAIEGVEAVSHANWFGGIYQDPKNFFGQ
                     MAVDGESYLALYPELVLPEEQKQAWMADRTGAIVGRVTAERFGWKVGDKIPIQGQIYP
                     RKDGSRTWEFNLAGIYEGKEKGTDTSNFFFHYDYLKEATRGDIGFVGWYIIRIADPAR
                     AQEIGERVDALFANSPAETKTQTEKAFAQSFANQVGDIGQIITAVLSAVFFTILLVAG
                     NTMAQSVRERTSELAVLKTLGFTHRQVLAMVLAESCLIALLGGLAGLGLAWLIIQQGD
                     PTKGALPVFFFPGRDIALGIALALILGLATGLFPAIQAMRLRIVEALRRV"
     gene            complement(2129..2809)
                     /locus_tag="VEL74_22785"
     CDS             complement(2129..2809)
                     /locus_tag="VEL74_22785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG65426.1"
                     /translation="MLVRVNDVNKIYQRGDERLEILRGLTLEVPQGDFLALMGPSGSG
                     KSTLLNLIGGLDRPTSGSVQVAGEPIDRLSDGQLAKWRARHIGFVFQFYNLLPVLTAE
                     RNVELPLLLTHLSKAQRMKHVATALSVVGLADRARHYPRQLSGGQEQRVGIARAIVTD
                     PTLLLCDEPTGDLDRKSGDEILDLLQALNTEHGKTIIMVTHDPHAAARARRTLHLDKG
                     SLTLETAA"
     gene            complement(2912..4159)
                     /locus_tag="VEL74_22790"
     CDS             complement(2912..4159)
                     /locus_tag="VEL74_22790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HYG65427.1"
                     /translation="MATEKSALDGLRIDRDSPAAGGSRRGGWILLGILLIGFIVAGWW
                     WFARSGAISVRAVTAREVGGGGAAGGAGEVLNASGYVVARRQATVSSKVTGKVTEILV
                     EEGMAVREGQVLARLDDSTLRKQLALTEAQAASSRRALAETAVRLREAEVNQRRMRRL
                     LAEGVTTQSQLDTADAEVDSLKARLELGRQDVEVADRQVAVQRQGLDDTVIRAPFSGV
                     AISKDAQPGEMISPVSAGGGFTRTGICTLVDMSSLEIEVDVNESYIKRVRPEQEARAV
                     LDAYPDWSIPSRVITIVPAADRQKATVRVRLAFQELDPRILPDMGVKVAFLGEEEPGQ
                     PAARQVVVPKAAVRKDGDRSIVFVVKDEKVERRAVQTRTGVGSGDDVTVISGLTGGER
                     VVVESPAELKDGDPVRIIEDAAK"
     gene            complement(4288..4659)
                     /gene="queD"
                     /locus_tag="VEL74_22795"
     CDS             complement(4288..4659)
                     /gene="queD"
                     /locus_tag="VEL74_22795"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HYG65428.1"
                     /translation="MHTIFKDFTFSAAHAIRGHTRGCQNLHGHNYRVRVHLRAERLDP
                     LGMVLDFADLKAIMQEVLGPFDHRVINDIPPFDERNTTAELFSEYVFEEVARRLPEPD
                     RVRISRVEVWENDTACAIFEP"
BASE COUNT          709 a         1635 c         1706 g          728 t
ORIGIN      
        1 tgcctccccc cagcagcgag aggaacagcg actcggcgag gaccgacagc accaccgggc
       61 cgccgccgaa gcccagggcg cgcagcgtcg cgatctcccg ggtgcgcgag gagaccgcgg
      121 tgtacatggt gttgagcgct ccgaacacag cacccacccc catcaggagg gcgatgatcg
      181 tccccaggcc ggtgatgatg ctgtagagga tccgcgactg gtcggcgtag tactcggtct
      241 cgcggatcac cttgacgtcg aggcgcggat cggtggtcag ggtgtccttg aagcggtcga
      301 aggacgcggg cgagtcgagc ttggcataga ccgcctggaa ggtgctgccg cgccggtagg
      361 ccggctgcag cactccggcg tcgcaccaga tctccgactc ggcgaggccg ccgccggctt
      421 cgaagatgcc caccacctgc cactcgctct ccccccagcg gtgccgcgcg ccgacttcga
      481 ggccggcgaa ctccgccgcc gccgcccggc cggcgatgat ctcgttgctg cccggccgga
      541 aggtgcgccc ctcgacgatc tcgacctcgt cgcgcacgtc gaaggcggcg gactggaccc
      601 cgcgcagggg gacgttggcg tcggtgccgg tggtgcgctt ggggaggtcg acgatgacga
      661 acagctcggc cgaggccatg ggcccgccgg cggagcgcgc cacgcccgga gcgtcggcga
      721 tcagccgcgt cgtctcccgc tccaggccgc tcatcatctc gctgtcgctg ccgctgcgca
      781 ggacgagggc ggtgtcctcg gagccggagg cggtcacggt ctggcggaac ccctcggcga
      841 tcgagagcac cgccacgaac acggcgacca cgccggccac gccgaacacc gccgcggccg
      901 aggcaccgag gcgctggccg atggtgcgca ggttgagccc ggtaaccgcc atcacttgcg
      961 agagccagtt catatcgtca taccctccgc agcgcttcga cgatgcgcag gcgcatggcc
     1021 tggatcgccg ggaagaggcc ggtggcgagg cccaggatca gggcgagggc gatgcccagc
     1081 gcgatgtccc ggccggggaa gaagaagacc ggcagcgcgc ccttggtcgg gtccccctgc
     1141 tggatgatca gccaggccag gccgaggccg gcgaggccgc cgagcagcgc gatcaggcag
     1201 gactcggcca gcaccatcgc cagcacctgg cggtgggtga agccgagggt cttgagcacc
     1261 gccagctcgc tggtccgctc gcgcaccgac tgggccatgg tgttgcccgc caccagcagg
     1321 atggtgaaga agaccgccga cagcaccgcg gtgatgatct ggccgatgtc ccccacctgg
     1381 ttggcgaacg actgggcgaa ggccttctcg gtctgcgtct tggtctccgc cggcgagttg
     1441 gcgaacaggg cgtcgacccg ctcgccgatc tcctgcgccc gggccgggtc ggcgatgcgg
     1501 atgatgtacc agccgacgaa gccgatgtcc cccctcgtcg cctccttgag gtagtcatag
     1561 tggaaaaaga agttggaggt gtcggtcccc ttctccttcc cctcgtagat gccggcgagg
     1621 ttgaactccc aggtgcgcga gccgtccttg cgcggataga tctgcccctg gatggggatc
     1681 ttgtccccca ccttccagcc gaagcgctcg gcggtcaccc ggccgacgat ggcgccggtg
     1741 cggtccgcca tccaggcctg cttctgctcc tcgggcagca cgagctcggg gtagagggcg
     1801 aggtagctct ccccgtccac cgccatctgg ccgaagaagt tcttcgggtc ctggtagatg
     1861 ccgccgaacc agttggcgtg gctcaccgcc tccaccccct cgatggccgc gatgcggtcc
     1921 ttgtaggaga tggggatggg ctggatgatg gagaccttgt ggaccaccag caggcggtcc
     1981 acgccgacca cgtccacccc catgctgaag gccatgcgga tggccgccag gtagccgaac
     2041 agcaggaagg cgaccaggat cgacagcagg gtgaagaggg tgcggacctt cttcctcttc
     2101 aggttgctcc agaggagggg gaggtacttc atgccgccgt ctccagggtg aggctcccct
     2161 tgtcgaggtg gagggtgcgg cgggcccgcg ccgcggcgtg cgggtcgtgg gtgaccatga
     2221 tgatggtttt gccgtgctcg gtgttgagcg cctggagcag gtcgaggatc tcgtccccgg
     2281 acttgcggtc gaggtcgccc gtcggctcgt cgcagaggag gagcgtcggg tcggtgacga
     2341 tggcgcgggc gatgcccacg cgctgctcct ggcctcccga gagctgccgc gggtagtgcc
     2401 gggcgcggtc ggcgagcccg accaccgaca gggcggtggc cacgtgcttc atccgctgcg
     2461 ccttggaaag gtgggtgagg agcagcggca gctcgacgtt gcgttcggcg gtcagcaccg
     2521 gcagcaggtt gtagaactgg aagacgaagc cgatgtggcg cgcccgccac ttggcgagct
     2581 ggccgtcgga cagtcggtcg atgggctccc ccgccacctg caccgacccg gaggtcggcc
     2641 ggtcgaggcc gccgatgagg ttgagcaggg tcgacttgcc cgagccagag ggacccatca
     2701 gggccaggaa gtccccctgc ggcacctcca gcgtgagccc cctgaggatc tccagccgct
     2761 cgtccccccg ctgatagatc ttgttgacgt cgttgacgcg gaccagcatg ccgttatcgg
     2821 ccacggttga cctcgctcgg aaagggttca gggaggagag gccggcccac gccgtccccc
     2881 tcctccattc tgagtgtcga ttctgttccg cctatttcgc ggcgtcttcg atgattctga
     2941 cgggatcgcc gtccttcagt tccgcgggac tctcgacgac cacccgctcg ccgccggtca
     3001 ggcccgagat cacggtcacg tcgtccccgg agccgacgcc ggtccgggtc tggaccgccc
     3061 gccgctccac cttctcgtcc ttgaccacga agacgatgga gcggtcgccg tccttgcgca
     3121 ccgccgcctt ggggaccacg acttggcgcg cggccggctg cccgggctcc tcctccccca
     3181 ggaaggccac cttgaccccc atgtcgggca ggatccgggg gtccagctcc tggaaggcga
     3241 ggcgcacccg caccgtcgcc ttctggcggt cggcggcggg gacgatggtg atcacccgcg
     3301 acgggatcga ccagtccgga taggcgtcga gcaccgcccg cgcctcctgc tccggccgca
     3361 cgcgcttgat gtagctctcg ttgacgtcga cctcgatctc cagcgagctc atgtcgacca
     3421 gggtacagat gccggtgcgg gtgaagccgc cgccggccga caccggcgag atcatctccc
     3481 ccggctgcgc gtccttggag atggcgacgc ccgagaaggg ggcacggatg acggtgtcgt
     3541 ccagcccctg gcgctgcacc gccacctgcc ggtcggcgac ctcgacgtcc tggcggccga
     3601 gctcgagccg cgccttgagc gagtcgacct cggcgtcggc ggtgtcgagc tgggactgag
     3661 tggtcacccc ctcggcgagc aggcggcgca tgcggcgctg gttgacctcg gcctcgcgca
     3721 gccgcacggc ggtctcggcg agggcgcggc ggctggacgc cgcctgggcc tcggtcaggg
     3781 cgagctgctt gcgcagcgtc gagtcgtcga ggcgggcgag gacctgcccc tcgcgcaccg
     3841 ccatgccctc ctcgaccagg atctcggtca ccttgcccgt caccttggag gagacggtgg
     3901 cctgccgccg ggccaccacg tagcccgagg cgttgagcac ctcgccggcg ccgccggccg
     3961 cgccgccgcc ccccacctcg cgcgccgtca cggcgcggac ggagatcgcc ccggaccggg
     4021 cgaaccacca ccagccggcc acgatgaatc cgatgagaag gatgccgagg aggatccagc
     4081 ccccccgccg cgatcccccc gccgccggac tgtcacgatc gatccgcagc ccgtcgaggg
     4141 cgctcttttc cgtagccatg tcgggtatta cctcgggagc cgcgcgcgcc cccccggcgc
     4201 tcggcggcct tcagtgactc cttacgtctc ggtgcggcaa aaagtttcgc gaggcgtagg
     4261 gagattctat ccgatccgga gggcctctca gggctcgaag atggcgcagg cggtgtcgtt
     4321 ctcccagacc tcgacgcggg agatgcggac gcggtccggc tcgggcaggc ggcgggccac
     4381 ctcctcgaag acgtactccg agaagagctc ggcggtggtg ttgcgctcgt cgaagggggg
     4441 gatgtcgttg atcacccggt ggtcgaaggg gccgaggacc tcctgcatga tcgccttgag
     4501 gtcggcgaag tcgaggacca tgcccagggg gtccaggcgc tcggcccgca ggtggacccg
     4561 cacgcgatag ttgtgcccgt ggaggttctg gcagccgcgg gtgtggccac ggatggcatg
     4621 ggcggcggag aaggtgaagt ccttgaagat ggtgtgcatg gccggtcctc atcctgtccc
     4681 cccagggctg agggagtcaa gagcggagcg agtcgagagg ggcggaaagg tccggcggcc
     4741 gggcggaacc ggtcaggtcg cctggaggaa ggcgacgg
//
LOCUS       DASPJI010000808         6411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_452333, whole genome shotgun sequence.
ACCESSION   DASPJI010000808 DASPJI010000000
VERSION     DASPJI010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452333"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VEL74_22800"
     CDS             <1..922
                     /locus_tag="VEL74_22800"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HYG65429.1"
                     /translation="VQNALTLLKVGSILAFGALGLLVPARAEARLFESATGVAGGIGG
                     GLLTAFGVAMIAALWTYDGWYGLTCSAGEVRDPARSLPRGLILGTVAVTALYLLLNLA
                     YVRALPMEAMAAAPRIGEAAAAALFGPGAARLVSLAVLVSTFGCLSATILYSSRIYLP
                     MAQDGLFFRRLGEVHPRFRTPVPSLWAQTAWSLVLVFSGTYQQLYTYVIFASILFHAG
                     TAAAVFVLRRRRPDLPRPYRVWGYPWVPALFILACLLLIGNTLAESPVESLAGLGLVA
                     LGLPAYGWMWRGGKTLQPEAAPSVSPGREV"
     gene            1036..4359
                     /locus_tag="VEL74_22805"
     CDS             1036..4359
                     /locus_tag="VEL74_22805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HYG65430.1"
                     /translation="MKRTLLAVALLPLLLAAPVLAAEKPAKDQKAGAGKDSKKSEKKE
                     LWSSGTFGGLRLREIGPALTSGRIGDIAVDPEDKNTWYIAVASGGLWKTTNAGVTFKP
                     IFDSQASYSIGCVTVDPNDPLTVWVGSGENNSQRSVGYGDGVYKSTDGGQSWTNVGLK
                     NSEHIGKILVDPRNSDVVYVAAQGPLWNPGGDRGLYKTTDGGKTWKPVLTISENTGVS
                     DIAFDPRDPDVLYATAYQRRRHVWTLINGGPESAIHKTRDGGVTWEKLTKGLPKVDMG
                     RIGLAVSPVDPDVVYAIVESVGTAGGFFRSQDGGGNWSKMSGYVSGSPQYYQELFADP
                     EDVDRVYSMDVFMKVTDDGGKTFRNAGERNKHVDNHALWIDPDDVDHLLVGSDGGLYE
                     SFDRGETWRFFPNLPVTQFYRVSADTTTPFYHVYGGTQDNFSLGAPARTLSRSGITNY
                     DWYITQGGDGFETQVDPKDPNIVYAQAQHAGIVRFDKKNGETVDIRPQTGKGEPGLRF
                     NWDSPLLISPHSHTRLYLAANRVFRSDDRGNSWRAISPDLTRQLDRNKLEVMGKVWSV
                     DTVAKNASTSFYGNIVSLTESPRVENLIYIGTDDGLVQVSADAGQTWRKIERFPGVPD
                     ETYVSRLEASLHDADTVYAAFNNHKNGDFKPYLLKSTDRGATWRSIAGDLPERGSVWA
                     LVEDHVNPDLLFAGTEFGAYFTTDGGRKWLRLTGGLPTIAVRDLEIQRREDDLIVGTF
                     GRGIWILDDYTPLRTANAQSVEKEATLLPVKKTWMYIENTPLGLAGKAFQGESFYVAS
                     NPPVGAVLTYYLKDEIKTRKATRQELERSREGEGQVLSYPSWEDLRAEDREKEPAILL
                     TVKDEDGNVVRRLTGPVSAGFHRLTWDLRYPAATPVRLTPPAESLFSQPDVGPLVAPG
                     RYTVTMAKEVDGVVTPLGEPQTFEATPLGMATLPVADRPALQAFQQKTARLQRAVEGA
                     KRLLTDTQERIDHLQQAVLETPGADPKLAEQALALERRLEDLNVALTGDDTIGRRNEP
                     TPASISERVDNIVSGWYTTAAPTGTHQEAYAVAAAEFAPVLAGLRQLVEQDLRQLETQ
                     LEAAGAPWTPGRVPNWQPE"
     gene            complement(4418..4993)
                     /locus_tag="VEL74_22810"
     CDS             complement(4418..4993)
                     /locus_tag="VEL74_22810"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013665648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HYG65431.1"
                     /translation="MNVQQGWIDRSAGHIEAITGGMFSGKSEELVRRLRRAAIARQKV
                     QVFKPEADTRHHPERLVTRDKRELEAVTVGCIEELRRKLQPGVQVVGIDEAQFFDTGL
                     IELVGELADSGIRVIVAGLDLDYLRRPFGPMAQILAIAEYVDKMHAVCMRCGAPAHYS
                     QRVAGGTEQLQVGDGEAYEARCRACFEVFHP"
     gene            5054..5407
                     /locus_tag="VEL74_22815"
     CDS             5054..5407
                     /locus_tag="VEL74_22815"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 family protein"
                     /protein_id="HYG65432.1"
                     /translation="MNPNRSHRRIFSYDEALATFPLIRDLTAAAVRQVEALVNRVGSR
                     DEMDTRREELEDACEQIVRAWAEEVGAVGCEVKGLWLVDWDSGDGYYCWKFPEESISY
                     FHTYEEGFAGRIPIN"
     gene            complement(5433..6140)
                     /locus_tag="VEL74_22820"
     CDS             complement(5433..6140)
                     /locus_tag="VEL74_22820"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HYG65433.1"
                     /translation="MLRAILFDFNGIIVDDEPIHLEMFQKVLGEAGVALSTEDYYERY
                     LGMDDRGCFAAVLDAAGETLTVPRLMRLIARKASYYQERIRERGYPIFPGAVELVRAA
                     ADGGRMLGVVSGALREEVEGALRQIGLRDRFKVLVTAEDVAEGKPDPEGYRRALEALN
                     GLPPLPERLIHPHEVLAIEDSPAGLEAAAEVGLVTLGIAHTYPTAQLGMADAVTERLA
                     GLTLDRLERLFAEASRS"
BASE COUNT         1022 a         2291 c         2156 g          942 t
ORIGIN      
        1 ggtgcagaac gcccttaccc tgctaaaagt cggctcgatc ctcgccttcg gcgcgctggg
       61 cctgctggtg ccggcgcggg cggaggcccg cctcttcgag agcgcgaccg gggttgccgg
      121 tgggatcggc ggcggtctgc tgacggcgtt cggagtcgcc atgatcgccg cgctgtggac
      181 ctatgacggc tggtacggcc tgacctgctc ggcgggagag gtgcgggacc cggcgcgcag
      241 cctgccgcgg gggctgatcc tcgggacggt ggcggtgacc gccctctatc tgctgctcaa
      301 cctggcctat gtgcgggcgc tgccgatgga ggccatggcg gcggcgccgc gcatcggcga
      361 ggcggcggcc gcggcgctgt tcggaccggg ggcggcgcgg ctggtgtcgc tggcggtgct
      421 ggtctccact ttcggctgcc tctcggcgac catcctctac agctcgcgga tctacctgcc
      481 catggcccag gacggcctct tcttccggcg cctgggggag gtccacccgc ggttccggac
      541 gccggtcccc agcctctggg cgcagacggc ctggtcgctg gtgctggtct tctcggggac
      601 ctaccagcag ctctacacct acgtgatctt cgccagcatc ctcttccacg ccggcacggc
      661 cgcggcggtc ttcgtcctgc gccgccgccg gccggacctg ccgcgcccgt accgtgtctg
      721 gggctatccc tgggtgccgg cgctcttcat cctggcctgc ctgctgctca tcggcaacac
      781 cctggccgag agcccggtcg agtccctcgc cggcctgggg ctcgtcgccc tcggcctgcc
      841 ggcgtacggg tggatgtggc ggggaggaaa gaccctgcaa cctgaggccg cgccatccgt
      901 atctccgggg agagaggtgt gacgcggtcg gacccagggg cccgcgatag cggttccctg
      961 tcgtttgttt ttgtggcaca tcccgcccgg acgctccgtg ccggacggtg gattgaacga
     1021 atcgggagga actcagtgaa acgcactctc ttggcagtcg ccctgctgcc gctcctgctg
     1081 gcggcccctg tgctcgccgc ggaaaagccg gcgaaggacc agaaggccgg tgccggcaag
     1141 gactcgaaga agagcgagaa gaaggagctg tggagctccg ggacgttcgg cggcctgcgg
     1201 ctgcgcgaga tcggccccgc cctcacctcc ggccggatcg gcgacatcgc ggtcgatccc
     1261 gaggacaaga acacctggta cattgcggtc gcctcgggcg gcctctggaa aaccaccaac
     1321 gccggcgtca ccttcaagcc gatcttcgac tcccaggcct cctactccat cggctgcgtc
     1381 accgtcgacc ccaacgaccc tctgaccgtc tgggtgggca gcggcgagaa caacagccag
     1441 cgtagcgtcg gctacggcga cggggtctac aagtccaccg acggcggcca gagctggacc
     1501 aacgtcgggc tcaagaattc cgagcacatc ggcaagatcc tggtcgaccc ccggaattcg
     1561 gacgtcgtct acgtggccgc ccagggcccg ctctggaacc ccggcggcga ccgcggcctc
     1621 tacaagacga ccgacggcgg caagacctgg aagcccgtgc tcaccatcag cgagaacacc
     1681 ggcgtctcgg acatcgcctt cgatccccgc gaccccgacg tcctctatgc caccgcctac
     1741 cagcggcgcc ggcacgtctg gacgctgatc aacggcggcc ccgagtcggc gatccacaag
     1801 acccgggacg gcggcgtcac ctgggagaag ctgaccaagg ggctccccaa ggtggacatg
     1861 ggccgcatcg gcctcgccgt ctcgccggtg gacccggacg tggtctacgc catcgtcgag
     1921 tcggtgggca ccgccggcgg cttcttccgt tcccaggacg gcggcggcaa ctggagcaag
     1981 atgagcggct atgtctccgg cagcccgcag tactaccagg agctcttcgc cgatcccgag
     2041 gacgtggacc gcgtctactc gatggacgtc ttcatgaagg tgaccgacga cggcggcaag
     2101 accttccgca acgccgggga gcgcaacaag cacgtggaca accacgcgct ctggatcgac
     2161 cccgacgacg tcgaccacct gctggtgggg agcgacggcg gcctctacga gagcttcgac
     2221 cgcggcgaga cctggcgctt cttccccaac ctgccggtca cccagttcta tcgcgtcagc
     2281 gccgacacca cgaccccctt ctaccacgtg tacggcggca cccaggacaa cttcagcctc
     2341 ggcgccccgg cccgcaccct gtcgcgcagc ggcattacca actacgactg gtacatcacc
     2401 cagggcggtg acggtttcga gacccaggtc gaccccaagg accccaacat cgtctatgcc
     2461 caggcgcagc acgccggcat cgtccgcttc gacaagaaga acggtgagac ggtggacatc
     2521 cggcctcaga ccggcaaggg ggagccgggc ctgcgcttca actgggactc gccgctgctc
     2581 atcagcccgc actcccacac ccggctctac ctggccgcca accgcgtctt ccggagtgac
     2641 gaccgcggca actcctggcg cgccatcagc ccggacctca cccgccagct cgaccgcaac
     2701 aagctcgagg tcatgggcaa ggtgtggagc gtcgacaccg tggccaagaa cgcctccacc
     2761 tctttctacg gcaacatcgt ctcgctgacc gagtcgccgc gggtggagaa cctgatctac
     2821 atcggcaccg acgacggcct ggtccaggtc tccgctgacg cgggccagac ctggcgcaag
     2881 atcgagcgct tccccggcgt gcctgacgag acctatgtct cccgtctcga ggcctcgctc
     2941 cacgacgccg acacggtcta tgccgccttc aacaatcaca agaacggcga cttcaagccc
     3001 tatctcctca agagcaccga ccgcggagcc acctggaggt ccatcgccgg cgacctgccg
     3061 gagcgtggct cggtgtgggc cctggtcgag gatcacgtca acccggacct gctcttcgcg
     3121 ggcaccgagt tcggcgccta cttcaccacc gacggcggcc gcaagtggct ccggctgacc
     3181 ggcggcctcc ccaccatcgc cgtccgcgac ctggagatcc agcgccgcga ggacgacctg
     3241 atcgtcggca ccttcggccg cggcatctgg atcctcgacg actacacgcc gctgcgcacc
     3301 gccaacgccc agtcggtaga gaaggaggct accctcctcc cggtcaagaa gacctggatg
     3361 tacatcgaga acacccccct gggcttggcc ggcaaggcct tccagggaga gtcgttctat
     3421 gtggcgtcca acccgcccgt aggcgcggtg ctcacctact acctcaagga cgagatcaag
     3481 acccgtaagg cgacgcgcca ggagctggag cgcagccgcg agggggaggg gcaggtgctc
     3541 tcgtacccga gctgggagga cctgcgggcc gaggaccggg agaaggagcc ggccatcctg
     3601 ctcaccgtca aggacgagga cggcaacgtg gtccggcgtc tcaccgggcc ggtcagcgcc
     3661 ggcttccacc ggctcacctg ggacctgcgc tatccggcgg ccaccccggt ccggctcacg
     3721 ccgcccgcgg agagcctctt ctcccagccg gacgtaggcc ccctggtggc gcccggccgc
     3781 tacaccgtca ccatggcgaa ggaggtggac ggcgtcgtca ctccgctggg cgagccccag
     3841 accttcgagg ccacgccgct ggggatggcc acgctgccgg tggcggaccg gcccgccctc
     3901 caggccttcc agcagaagac cgcgcgcctc cagcgggccg tcgaaggggc gaagcggctc
     3961 ctgaccgaca cccaggagcg catcgatcac ctgcagcagg cggtgctgga gactcccggg
     4021 gccgatccga agctggccga gcaggcgctg gccctggagc ggcggctgga ggacctgaac
     4081 gtcgcgctca ccggcgacga caccatcggc cgccgcaacg agccgacgcc ggcctcgatc
     4141 agcgagcggg tcgacaacat cgtgagcggc tggtacacca ccgcggcgcc caccggcacc
     4201 caccaggagg cctacgcggt ggccgcggcg gagttcgctc ccgtgctggc gggcctccgt
     4261 cagctggtgg agcaggacct gcgccagctc gaaacccagc tcgaggccgc cggcgccccg
     4321 tggacgccgg gccgggtccc gaactggcag cccgagtagg gcgcgccgca tccgccacac
     4381 cccgggcggg ggcctccccc gtccggggtt tttctattca ggggtggaag acttcgaagc
     4441 aggcgcggca gcgggcctcg taggcttcgc cgtccccgac ctggagttgc tcggtgccgc
     4501 cggccacgcg ctggctgtag tgggcgggcg cgccgcagcg catgcagacc gcgtgcatct
     4561 tgtcgacgta ctcggcgatg gcgaggatct gcgccatcgg cccgaacggc cgccgcaggt
     4621 agtcgaggtc gaggccggcg acgatcaccc ggatgccgga atcggccagc tcgccgacca
     4681 gctcgatcag gccggtgtcg aagaactggg cctcatcgat ccccaccacc tggacgcccg
     4741 gctggagctt gcgccgcagc tcctcgatgc agccgacggt gaccgcctcc agctcccgct
     4801 tgtcgcgggt gaccagccgc tccgggtggt gccgggtgtc ggcctcgggc ttgaacacct
     4861 ggaccttctg gcgggcgatg gccgcccggc gcaggcgccg caccagctcc tcgctcttgc
     4921 ccgagaacat gccgccggtg atggcctcga tgtggccggc ggagcgatcg atccagccct
     4981 gttgtacgtt cacgtccgaa gaaatctacc gtaaccgggc gctcacgcca aggtctgcta
     5041 gcatctgcct gtgatgaatc cgaaccgtag ccaccgccgg atcttctcct acgacgaggc
     5101 gctggcgacg ttccccctga tccgggacct caccgcggcg gcggtccgcc aggtcgaggc
     5161 gctggtcaac cgggtcggca gccgcgacga gatggacacc cgccgcgagg agctggaaga
     5221 cgcctgcgag cagatcgtcc gcgcctgggc cgaggaggtg ggggccgtcg gctgcgaggt
     5281 caaggggctc tggctggtgg actgggacag cggcgacggc tactactgct ggaagttccc
     5341 cgaggagtcg atctcctact tccacaccta cgaagaaggc ttcgccggcc gcatccccat
     5401 caattagata gagaaaaggg tggaaggccg ggctagctcc ggctggcctc ggcgaacagc
     5461 cgctcgagcc ggtccagggt gagcccggcc aggcgctccg tcacggcgtc ggccatgccg
     5521 agctgggcgg tggggtaggt gtgggcgata cccagggtga cgagccccac ctcggcggcg
     5581 gcttcgaggc ccgccgggct gtcctcgatg gccagcacct cgtgcggatg gatgagccgc
     5641 tccggcagcg gcggcaggcc gttcaaggcc tccagcgccc gccggtagcc ttcggggtcc
     5701 ggcttgccct cggccacgtc ctcggcggtg acgagcacct tgaagcggtc gcgcaggccg
     5761 atctgccgca gggccccctc cacctcctcg cgcagcgcgc cgctcaccac gccgagcatc
     5821 cgaccgccgt ccgccgccgc ccgcacgagc tcgacggcgc cggggaagat ggggtatccc
     5881 cgctcgcgga tgcgctcctg gtagtagctc gccttgcggg cgatgaggcg catcaggcgc
     5941 ggcaccgtca gcgtctcgcc ggcggcgtcg aggaccgccg cgaagcagcc gcggtcgtcc
     6001 atgcccaggt agcgctcgta gtagtcctcg gtgctgagcg cgacgcccgc ctcgcccagg
     6061 accttctgga acatctccag gtggatgggc tcgtcgtcga cgatgatgcc gttgaagtcg
     6121 aagaggatgg cgcggagcac ggggagatca tatcaacccc gggagccctc ccgggagaga
     6181 aaagggaaca aaagggccgt tcaggccgtt ggagagctgg gagaggcgcg ctccgcgcgg
     6241 cttcttgcac tactatcttg tacgagatct agaccctctg ccttggaggg aggagcaccg
     6301 atggagaata tcgagcaggt tcagcccact gcgactccgg ctcagccggc cgaggaggag
     6361 actcccgtca cgctgacgcc caaggcggtg gagatggtca ggatcacccg c
//
LOCUS       DASPJI010000809         5371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_729797, whole genome shotgun sequence.
ACCESSION   DASPJI010000809 DASPJI010000000
VERSION     DASPJI010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729797"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>5371
                     /locus_tag="VEL74_22825"
     CDS             <1..>5371
                     /locus_tag="VEL74_22825"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65434.1"
                     /translation="ELTAGKFLPDPWSATPGARLYRTGDRARYRPDGNLEFLGRRDHQ
                     VKIRGFRIELGEIEAALATLPGVREAVVVVREDTPGDRRLVAYVTGDPLVDDLRPWLR
                     ERLPEPMMPSAFVSLPALPLTPNGKVDRKALPAPDRQRAEEAYVPPRTPIEEIVAGIW
                     AEVLGLERVRAADHFFELGGHSLLATRVVSRLREAFGVELPLRDVFELPNLQDLAGRV
                     ETVRGGGRAAPLPALVPVPREGDPPLSFAQERLWFLDQLEPGSPAYDIPGAVELTGRL
                     DLAALSAALDCLVARHEILRTVFRVIDGQPRQHVLPSLEIGLPVLDLAGLPPDRRQAE
                     AERLAAEQALVRFDLGRGPLLESLLLRLDGTGDRHRLLVVVHHVICDGWSLPLLVREV
                     GELYAACVAGRPSPLAELPVQYADFAIWQHAVVAASEQTELAWWLERLGGEIAPLELP
                     TDRLRPPVQTYRGRQSTRVLPPALAARLTAFGRAHGATPFMTLLAAIQALLHRHSGQD
                     DILVGAPVAGRRAVETEPLIGCFLNTLVLRTGLGGEPGFRDLVARVREVTLGAFSHQD
                     VPFEAILARLPQPRDLSRTPLFQVMVNLLNLPSTEMRLPGLALAGSTTVTPLSKLDMT
                     FYVTEGSGGARIELVYNADLFDAARMEDLLAQLEALLGRALEWPETPVGGLSLVTAAA
                     RAVLPDPAAALSAAWEGAVHEILARHARQAPGALAVADPAESWTYGELEERSSRLAAL
                     LRAGGVGPGDVVALWAHRSAPLVWGLLGVLKAGAAFLVLDPRHPAPRQVQMLSLARPA
                     AWLQAAAAGPVPAEIAGELDAVGCACRLPLPARAEESSGFLARVPAVAPDVEVGPDDA
                     AYVAFTSGSTGVPKGVVGRHGSLSHFIPWLSRRFELSAGDRFSLLSGLAHDPLHRDVF
                     TPLQIGAAVVIPDPETMEEPGELAAWMRREKVTVAHLTPALGQMLTTEATDGSGIEVP
                     SLRYAFLVGDVLTRRDVARLRRLAPAVTCVNYYGSTETQRAVGCHVAEEGPDGARVKE
                     VLPLGRGIPDVQLLVLNRAGALAGIGELGEISMRSPHIALAYLGDPRLTAQRFAPNPL
                     GGREDGRLYRTGDLGRYLPNGEAVFAGRADTQVKIRGFRIELGDVEAALGAFPGVREA
                     VAVAREDGGGERSLAAYVVPAAGAILDERELRAFLRRRLPDFMVPATFTLLDRLPLTP
                     NRKVDRKALPAPEQRRSDHWTAPRTPVEEALAGIWAELLGRERVGADEDFFALGGHSL
                     LATRLVSRLRGAFDIEMPLRALFEAPVLADLAARVEAARHEGASRHAPPLVSVPRRGP
                     LPLSFAQQRLWFIDQLEPGSPLYNVPMALRVEGALATEVLARCLGEIVRRHEALRTVF
                     GTRDGSPVQVIQPAAPFTLAVVDLSALSAARREAAALALAREEADRPFDLARGPLLRA
                     LLLRLDSQDHLAAVTQHHIVSDGWSLGILVDEVAALYAAFAAGRPSPLTELRVQYADF
                     AVWQSSWLRGETLEREISYWRRQLADLPPRLELPTDRPRPAAQSFRGTTRPARLPAGL
                     TGKARDLGRREGATLFMVLLAGFQTLLARVSGQQDFAVGTPVAGRNRTEIEGLIGFFV
                     NALVLRAGLRGEPTFRELLDRVRETTLAAQSHQDVPFERLIQELAPERNLAQTPLFQV
                     VLALQNAAAESRRIEGLRVRQMSTTGTTAKFDLTLNLVEHAGGLGGTVEHSIDLFDAT
                     TIERLLRQYERLLAAAVETPDLQVRDLPLLPDAERHQVLVEWNDTRVGRGESTLIHEL
                     FEAWAA"
BASE COUNT          630 a         1996 c         1942 g          803 t
ORIGIN      
        1 cggagctgac cgccggcaag ttcctccccg acccgtggag cgccacgccc ggcgcccggc
       61 tctaccgcac gggcgaccgc gcccgctacc gtccggacgg caacctggag ttcctggggc
      121 ggcgggatca tcaggtcaag atccgcggct tccgcatcga gctgggggag atcgaggcgg
      181 cgctcgccac ccttcccggc gtgcgcgagg cggtcgtggt ggtgcgggag gatacccccg
      241 gcgaccggcg gctggtcgcg tacgtgaccg gcgatcccct cgtggacgat ctccgcccct
      301 ggctgcggga gcggctgccg gagcccatga tgccgtcggc cttcgtgtcg ctgcccgccc
      361 tacccctgac cccgaacggc aaggtggatc gcaaggcact gccggcgccg gaccggcagc
      421 gcgccgaaga ggcctacgtg ccgccgcgca cgccgatcga ggagatcgtg gccgggatct
      481 gggcggaggt gctcggcctc gagcgggtga gggcggccga ccacttcttc gagctgggcg
      541 gccattcgct gctggccacc cgggtcgtct cgcggctacg ggaggccttc ggtgtcgagc
      601 tgccgctgcg cgacgtcttc gagctcccca acctccagga tctcgccggg cgggtggaga
      661 cggtgcgcgg cgggggccgt gcggctccgc tccccgccct cgttccggtg ccgcgggagg
      721 gagatccacc cctctccttc gcccaggagc gcctctggtt cctcgaccaa ttggagccgg
      781 ggagcccggc ctacgacatc ccgggcgcgg tggagctcac cggccgcctc gacttggcgg
      841 ccctctccgc cgcgctcgac tgtctggtcg cccgccacga gatcctgcgc acggtcttcc
      901 gggtgatcga cgggcagccc cgccagcacg tcctgccgtc gctggagatc gggctgccgg
      961 tgctcgatct cgccgggctg ccgccggacc gccggcaggc cgaagccgaa cggctggcgg
     1021 cggagcaggc cctggtccgc ttcgatctgg gccggggccc gctgctggag agcctcctcc
     1081 tgcggctaga cgggacgggg gaccgccacc gcctcctggt cgtcgtgcat cacgtgatct
     1141 gcgacggctg gtcgctcccc ctcctggtgc gcgaggtcgg ggagctctac gcggcctgcg
     1201 tggccggcag gccctccccg ctggcggagc tgccggtcca gtatgccgat ttcgccatct
     1261 ggcagcacgc cgtcgtcgcc gcctcggagc agaccgagct cgcctggtgg ctggagcggc
     1321 tgggcggtga gatcgctccc ctcgagctgc ccaccgaccg gctccgcccg cctgtgcaga
     1381 cctaccgcgg ccggcagagc acgcgcgtcc tgcctcccgc tctcgccgcc cggctgaccg
     1441 ccttcggccg ggcacacggg gccacgccgt tcatgaccct gctcgccgcc atccaggcgc
     1501 ttctccaccg ccacagcggc caggacgaca tcctggtggg ggcgccggtc gccggccgcc
     1561 gcgcggtgga gaccgagccg ctgatcggct gctttctcaa caccctggtc ctgcgcaccg
     1621 gcctcggcgg cgagcccggg tttcgcgacc tcgtggcgcg ggtgcgggag gtcacgctcg
     1681 gcgccttctc gcaccaggac gtacccttcg aggcgatcct cgcccgcctg ccccagccgc
     1741 gcgacctctc ccggacaccg ctcttccagg tgatggtcaa cctgctcaac cttccgtcca
     1801 cggagatgcg gctgccggga ttggcgctcg cgggctcgac gacggtgaca cccctctcca
     1861 agctcgatat gaccttttac gtcaccgagg ggagcggcgg agcgcggatc gagttggtct
     1921 acaacgccga tctgttcgat gccgcgcgca tggaggacct cctcgcccag ctcgaagcgc
     1981 tgctgggccg agcgttggag tggcccgaga cgccggtcgg cgggctgtcg ctggtcacgg
     2041 ccgccgcccg cgcggtcctg cccgatccgg ccgccgcgct gtcagccgcc tgggaggggg
     2101 cggtgcacga gattctcgcg aggcatgccc gccaggcgcc cggagccctc gccgtagccg
     2161 atccggcgga gtcctggacg tacggcgagc tcgaggagcg gtccagccgg ctcgcggctc
     2221 tgctgcgtgc gggcggtgtc gggcccggcg atgtggtggc cctctgggcg catcgcagcg
     2281 cgcctctcgt ctggggcctc ttgggtgtcc tgaaggcggg cgccgccttc ctggtgctcg
     2341 acccgcgcca tccggcgccg cgccaggtcc agatgctgtc gttggcccgg ccggccgcct
     2401 ggctccaggc tgcggcagcg ggacccgtgc cggcggagat cgcgggtgag ctcgacgccg
     2461 tcggctgcgc ctgccgcctg ccgctgccgg cccgggcgga ggaatcctcc ggcttcctcg
     2521 cccgcgtccc cgccgtcgct ccggatgtgg aggtggggcc ggatgacgcc gcctatgtcg
     2581 ccttcacctc cggctcgacg ggggtcccca agggggtggt cggccgtcac ggctcgttgt
     2641 cgcacttcat cccctggctg agccgccgct tcgagctgtc ggccggcgac cgcttcagcc
     2701 tcctgtcggg gctggcacac gacccgctcc accgcgacgt cttcacgccg ctgcagatcg
     2761 gggccgcggt ggtgatcccc gaccccgaga ccatggagga gcccggggag ctcgcggcct
     2821 ggatgcgccg cgagaaggtg accgtcgcgc acctcacgcc cgccctcggc cagatgctca
     2881 ccaccgaagc cacggacggc tccgggatcg aggtcccgtc gctgcgctat gccttcctgg
     2941 tgggggacgt cctcacgcga cgcgacgtgg cgcggctgcg ccgcctcgcc cccgcggtca
     3001 cctgcgtcaa ctattacggc tccaccgaga cccaacgcgc cgttggctgt cacgtggcag
     3061 aggagggacc ggacggagcc cgggtcaagg aggtcctgcc gctcgggcgc ggcattccgg
     3121 acgtccagct cctggtcctc aaccgggcgg gagccctggc gggcatcggc gagctcggcg
     3181 agatctcgat gcgcagcccg cacatcgccc tcgcctacct gggggacccg cggctcaccg
     3241 cgcaacgctt cgcgcccaac ccgctcggcg gccgggagga cggccgcctc taccgcaccg
     3301 gagacctcgg gcgctacctg cccaacggcg aggcggtctt cgcagggcgc gccgacacgc
     3361 aggtcaagat ccgcggcttc cgcatcgagc tcggcgacgt cgaggcggcg ctcggcgctt
     3421 tcccgggtgt ccgcgaagcg gtggccgtgg cgcgggagga tgggggaggg gagcggtccc
     3481 tggccgccta tgtggttccc gccgccggcg ccatcctcga cgagcgggag ctgcgcgcct
     3541 tcctgcgccg ccggctgccg gacttcatgg tgccggccac tttcacgctc ctcgaccggc
     3601 tgccgctgac gccgaaccgg aaggtggaca ggaaggcgct gccggctccg gagcagcgcc
     3661 gctccgacca ttggacggct ccccggacgc cggtggagga ggccctcgcc ggaatctggg
     3721 cggagctgct cgggcgcgaa cgggtggggg cggacgagga cttcttcgcc ctcggtggcc
     3781 attcgctgct ggctacgcgg ctggtgtcgc ggctgcgcgg cgccttcgat atcgagatgc
     3841 ccctgcgcgc gctgttcgag gcccctgtgc tggcggacct cgccgcccgg gtggaggcgg
     3901 cgcggcacga gggcgcgagc cggcatgccc cgcccctggt gtcggtgccg cggagaggtc
     3961 cgctccccct ttcgttcgcg cagcagcgcc tctggttcat cgatcagctc gaaccgggga
     4021 gcccgctcta caacgtgccg atggcgctgc gcgtcgaagg ggcgttggct accgaggtgc
     4081 tggcgcgctg cctcggcgag atcgtgcgcc ggcacgaggc gctgcgcacg gtcttcggca
     4141 cgcgggacgg ctcgccggtg caggtgatcc agccggcggc gccctttacc ctggctgtag
     4201 tggatctgtc ggcgctgtcc gcggcccggc gtgaggcggc ggccctcgcc ctcgcccggg
     4261 aagaggccga ccgcccgttc gatctcgcgc gcgggccgct gctgcgcgcc ctcctgctgc
     4321 ggctcgacag ccaagaccac ctcgccgcgg tcacccagca tcacatcgtc agcgacggct
     4381 ggtccctggg gatcctcgtc gacgaagtgg cggcgctcta tgccgcgttc gccgcgggcc
     4441 gtccctctcc cctgaccgag ctgcgggtgc agtatgcgga cttcgcggtc tggcagtcct
     4501 cctggctgcg cggcgagacc ctggagcgcg agatctccta ctggcggcgg caactcgcgg
     4561 acctgccgcc gcggctggag ctccctaccg atcgcccccg gcccgcggcc cagagcttcc
     4621 gcggcaccac gcggcccgcg cggctgccgg ccggtcttac cggcaaggcg cgggacctgg
     4681 gccggcgcga gggggcgacc ctgttcatgg tgctcctcgc cggcttccaa acgctgctcg
     4741 cgcgggtcag cggccagcag gacttcgcgg tgggcactcc ggtcgccggt cgcaaccgga
     4801 cggaaatcga agggctgatc ggcttcttcg tcaacgccct ggtgctgcgc gctggcctgc
     4861 gcggcgagcc gacgttccgc gagctgctcg accgggtccg tgagaccacg ttggcggccc
     4921 agtcgcacca ggacgtgccg ttcgagcggc tcatccagga gctcgcgccg gagcggaacc
     4981 tcgcgcagac ccctctgttc caggtggtgc tggcgctgca gaacgccgcc gcggagagcc
     5041 ggcgcatcga gggcctgcgg gtgcggcaga tgagcacgac ggggaccacg gcgaagttcg
     5101 atctcacgct gaacctcgtc gagcacgccg gagggctcgg cgggaccgtc gagcacagca
     5161 tcgatctgtt cgacgccacg accatcgagc gcctgctccg ccagtacgag cggctgctcg
     5221 cggcggcggt ggagacgccg gatctccagg tccgggacct gccgctgctg ccggatgccg
     5281 agcgccatca ggtgctggtg gagtggaacg acacccgggt gggccgtggt gaatccaccc
     5341 tgatccacga gctgttcgaa gcctgggcgg c
//
LOCUS       DASPJI010000810        17321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_161148, whole genome shotgun sequence.
ACCESSION   DASPJI010000810 DASPJI010000000
VERSION     DASPJI010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17321)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17321
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161148"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VEL74_22830"
     CDS             <1..1111
                     /locus_tag="VEL74_22830"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HYG65435.1"
                     /translation="FVIDSRWPRGRTSHWRLTVDDDWLRRPIGRDLPRSLTPTIRGGE
                     FLEYVSRHAMTSKHWHAFISVPVILGEYFLGALSLETRLEPIQHLVATKLSARYDAMA
                     PRLNFGVLHSAAAAAAYMWLRERDKLNPRHAAKLIESFASDSPAPPSPTYRRSPESDW
                     IKRRTVLNPEQLYEAGCHFGIFRKRASRIEIVNITPRLLDLLRESPEHLSQLSPGQFE
                     ELVAERLDRMGFDVALTGAASRPDGGIDLVATPKERNVASFLLAAQIKHHSAGRPTTR
                     ESIDRLLAWKDSVFRLGLVVTNTRFTEHARWVAMQNLDFLRLRDFDDLRRWLYDDFSS
                     DEDWGELPASVQLAPGIVVEIPRPSLLKMRPSRRS"
     gene            complement(1533..3215)
                     /locus_tag="VEL74_22835"
     CDS             complement(1533..3215)
                     /locus_tag="VEL74_22835"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HYG65436.1"
                     /translation="MRYSMGMGRRTERGNLGFGGKLRVLRGARGFSQTELAERSRVKR
                     PAISEYESDKALPDVATIERLLRGMRFKWSALDHAAAFLRSLESSEIEQEAPWGEETA
                     SLGAVVRVLRHARGFSQIGLAKESGVSRSAIAESEEDRGVPDASTIHRILGALGLAWS
                     ALDHAVSFLSRLRTAETEAANRVREEPVPPAAVLALAAARERAPYLWVRLTRHPSSNW
                     QEVVARGDEFKTWAFVEFLSIRSEELCSSEPREAIALAEFAGFVAEHVAGPESLQARS
                     QGFAAAHLGNAHRVSGKLKAAEEAFARSDELWASGEGAPDLFEESRLLDLKASLRRAQ
                     RRLSEALDLLDRALAIARNPAARARVLLKKSKTQEESGDWQGAITSLYEAAPDIGPAE
                     ERLLLILHHNLVWLLTNAGRHEEARQYFPKALDLSRCRGGTLDRIRLRWVEARMAAER
                     GETENAVTLFSKVRGEFVRRGMGFDTALISMELAVLYLQEGRTEDVKTLARQMIPIFK
                     AQDIHREALAALALFRQAVEREGATLEFTRRMLEYLRKARHNPELHFQPFAS"
     gene            complement(3212..4522)
                     /locus_tag="VEL74_22840"
     CDS             complement(3212..4522)
                     /locus_tag="VEL74_22840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65437.1"
                     /translation="MTANHLDPDALNHLLENHLDKEAWSRALLHLIAVCPECRKAGGA
                     ILDLYEAGAVDLDLCPIAIGLARSRAEAPALFAELEALPLEARRKTIESDPQWRSWGL
                     CEHLSDASERAAAEDPKKAVHFAELAVVLSLALEEWEPAEQLWLDELRANAYAYLGNA
                     QRVSGDLRAAEQSFLQSDRWWSRGGTQAGDVLGYEARILDLKASLRRAQRRLPESLEL
                     LEEALAAPEITPEMRGRVLIKKAKTLEEVGEVDRAIGVLRDAVPLVEEANDPRLLLCA
                     THNLMWLLAIAGRHLEAHLLVRSVSGLTASAGGRLDLLRLRWVEAQIAAGFGHLDEAV
                     KILEEVRQEFASQNLGYDAALVGLELATLRLREGRLEEVKILAREALPLFASQGVTRE
                     VLGAATLFLQAAEAETLTDEVARQVLEFVRRERSSPARSTTKEE"
     gene            5726..6634
                     /locus_tag="VEL74_22845"
     CDS             5726..6634
                     /locus_tag="VEL74_22845"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG65438.1"
                     /translation="MRYTRDGVPDLGQVRGEEMAKRALEVALAGGHHFLLKGLPGCGK
                     SLLLRCIPGLLPPLSPGEVRELADLGRGGARPFRPVRSPAPGITPHRMAGWSRRSPGE
                     AISAHGGVLVLDGLHAFRRDTLLSLLRPMDEGTVEARGERLPARFQVVGALAPAGRKR
                     LPPDLLDRMDLEVVMGAQAPPDLWAPREEDGTAEAAKRIAGARAIQAERFEPHPARIN
                     ARMSEDDILWYCVLDRASKSLLEEARARFHLSARSLLSIHKVARTIADLAGSDVIRAP
                     HLAEALQYRKLRTRKAMGEETQEGRE"
     gene            6631..7122
                     /locus_tag="VEL74_22850"
     CDS             6631..7122
                     /locus_tag="VEL74_22850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65439.1"
                     /translation="MSPVKLGEIRRAIEKETELLSEIAERLRNFAARVEAPTAEELRA
                     IEAGEAPMTAEAHLISVLTEQALSVGEAAVALRDTRHATTDLFRKAWAPGSRPFARAI
                     AHLRAGLRGRTLPADFRPEEPLGTHPWPPPAGSALVADLLAKGYRWEFDLPPLPQRER
                     KKS"
     gene            7171..8142
                     /locus_tag="VEL74_22855"
     CDS             7171..8142
                     /locus_tag="VEL74_22855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65440.1"
                     /translation="MREGLLRLRQRAALEVEALKARRMRGEVDPWDGDFLRDLTLAYR
                     GAGDEVGARITYPQACTSARRRKDDGLLLSLVRMTRLFLNPDSAESHLRWARELAREK
                     GFCGEEARCLNDLGVVALELRNFPLAHERLLASLGVFGALGENEETAVPLMNLACLGF
                     ARGDLAEARERLAVARVCAAGNLSRVLAIRLLQAILRAVEEKDFLGCLDELQEIAKEA
                     PASDDLETLLALGFALGRAYLLRGNPRSALDVALPPPRWGNDDALAYGALAALSLEAW
                     ETMDVAPLPETLEEAEALSRTTSLQAWRYRQTWVPRLVLSIDIPVRP"
     gene            8640..9128
                     /locus_tag="VEL74_22860"
     CDS             8640..9128
                     /locus_tag="VEL74_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG65441.1"
                     /translation="MNSCPCGHLGDPRHDCRCPLPLVERYRSRVSGPLLDRIDLHVEV
                     PAVSLKELRSDPGESTADVAARVAQARAVQLERFGTGSSTPVNAVMGPEALRRHCRVD
                     PAGRTLLDSAFEKLGLSARALDRVLRVARTIADLAGSDTIRSAHLAEAIQYRSLDRRV
                     TW"
     gene            9428..10360
                     /locus_tag="VEL74_22865"
     CDS             9428..10360
                     /locus_tag="VEL74_22865"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HYG65442.1"
                     /translation="MSKRHHTVIFVPHAHTKLRKWRISNLQIGVAVGALVLLTVAAAL
                     ALWSFFSTPSNPAEVARLRQENEQLRLANHRFETSIQSLQNQLGSYEERTRQLAIVAG
                     LENLGEGVEAGIGGGEPMPPIADLGAGGPAPGEEARGEIDATDLPEMASRAEHLAGTL
                     DQVQAKLDERMRWLSATPAITPVRGILTSGFGYRSDPVTHGRGVHQGVDIAAATGQPV
                     KASADGIVIRAGVEGGLGQAVFLSHGYGLTTRYGHLSRIDVRPGQRVKRGEVVGRVGN
                     TGRSTGPHLHYEVRVDGEPVNPLAYLYDHGAGGS"
     gene            complement(10463..14569)
                     /locus_tag="VEL74_22870"
     CDS             complement(10463..14569)
                     /locus_tag="VEL74_22870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG65443.1"
                     /translation="MRSRILNLALLLGTVSAPFLPLPWPALALALLTVLWLWTRPPRR
                     EVVVVTIALAVSATALVAGWLEERQARSSSRDWVGEARREYAELWDGLRDEAQRAVAA
                     VGRPPETREERLAAFSRLAALQPTRGRERRALLLLDPDGTAIAWAGEGLLHEPRPDSL
                     PRDGLHSAVGWSAVTFLAVAPLDEARRPWRIVAGASFPTHVLPFPGLDRPQRWSVVDD
                     PAQALPGAIQVRAEGAPTLVIDPALEGAPGAPAERPWARRVAWAAIAFALLSQAATRA
                     LGLILAPGVFPTGKIRERRFWIAANVLGGAFAAGMAAALPLFALIALEAGLLIAALGL
                     TGRHFAGRAGPPWGARWIPVWGVVTVLLLAAAACGLQWLRGPLDLSSSILVPPVAHAL
                     RLAWTAAAFGLLCFRGLTLPAPTRPRDRWAWLAVLLLLAGGMLSNHPWAALPLLAAGG
                     AVTAFYARHRRLGQGIGLGVLTLFSVLVAMGSWEVGYRLRLRAEAGPGLLDRLAPPSE
                     QAISELQKGLRAHFQSYDLRDLVPRADAGLERQDLAFALWRDSPLARPHALSAVVVEP
                     LEGVPSSFSFGIPLAAEGGIDTNSPSWQDLGLPLWEETLIPGQAELRFAGRPWGTVRY
                     WLMPRPGFQLRGDRLEDVEAGLLKGGPAASQVEDITGPALFALYWADGRVALSPWEES
                     PSLDPRLLREPRKVVRIGPVSTPSGPVYAFSRATRQGWEVIYLPLLTPLRSLERIANA
                     GIGTLFLLATVLPLLLLPSLPRSAFRDGVQRVMRSYSKRLLVVYTALLLLPLLLLSAF
                     LVQAMEQRLQEEQRAAGEAAMWSAQKVLGEYILLLPPGFILETELDDDLLSWISEVIH
                     HEVNLYWGSGVYASTKHELFTAGLLPRRIPGEIYSHLVLGGFALSSRTNVAGDTTYLE
                     LYAPVRVGNEAPGEGRLFLSIPLLAQQEEAARDLAQMRRQGVLVATGLFILLAAVGMR
                     LARNFTRPLMQLIEGTRRIAAGAPSLDLAPSDLELASLVAAVDEMARRIAEGRERLMR
                     EKQVVERMVDNITSGVVSVDRDRRVLMRNRVAAELLGVEVGENLEEAVARGERLGGVS
                     AFLGTVASEMARETVRLPADAGGEREWSLVWVPVPGSGEPSALLVVEDATEVLRGQRL
                     LAWAEMARMIAHEIKNPLTPIRLSAEHMREVYRSDPSHFDRVFDRCTSNILTQVEELR
                     SISAEFSAYSAIPRIAPRPGDLTAAVAELVEGYQAAPPEGVRVELETNDAIPARFDAK
                     LLGRAVRNLMENALRAAGASTGRSGEVVVRVERNDGIARIAVLDSGPGVRPENLQRIF
                     DPYFSTHDTGTGLGLPIARRIVEEHGGGIIARNRPEGGLEVTITLPVV"
     gene            complement(14626..16344)
                     /gene="hutU"
                     /locus_tag="VEL74_22875"
     CDS             complement(14626..16344)
                     /gene="hutU"
                     /locus_tag="VEL74_22875"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HYG65444.1"
                     /translation="MTTTIATEPRPEAASPRTVRAPRGTEISCRGWQQEAALRMLMNN
                     LDPEVAERPEELVVYGGSGKAARNWECFEAIVATLKRLGDDETLLVQSGKPVAVFRTH
                     TEAPRVLLANSMLVPRWATADEFRRLEGLGLTMYGQMTAGSWIYIGTQGILQGTYETL
                     AECARQHFGGSLAGRLTVTAGLGGMGGAQPLAVTMNEGVCLVAEVDRSRIERRLATRY
                     LDEVAPDIRTGVERALAARDRRQAVSIAVEANAVELLEHLVAAGLVPDALTDQTSAHD
                     ALQGYVPHGLRYDEALRLRQEDPETYIARSMESMAAHVRAMLVLQERGAVTFDYGNNL
                     RGQALQAGVDRAFKIGGFVPLFIRPLFCEGKGPFRWAVLSGDPEDLAVTDEAILATFP
                     EDQALARWIRLARERVAFQGLPARICWLGYGERARAGRVFNELVASGRVKAPIVIGRD
                     HLDCGSVASPNRETEGMLDGSDAIGDWPVLNAMLNAVNGATWVSVHHGGGVGIGYSLH
                     AGMVIVADGTEAAAARLERVLTGDPGMGVVRHADAGYPEAIEFGRRHGVDIPMLGRPG
                     ATGSGE"
     gene            16383..>17321
                     /gene="pncB"
                     /locus_tag="VEL74_22880"
     CDS             16383..>17321
                     /gene="pncB"
                     /locus_tag="VEL74_22880"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019357 - nicotinate nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HYG65445.1"
                     /translation="MGTLTRLYGERLALLTDLYQITMAYAYWKSGTFRKEAAFHLHYR
                     KNPFGGGYAVACGLEPVLDFLEGFRFGDEELGYLAGLRGNDGAPLFERSFLDYLREMT
                     FQCDVDAIPEGTVVFPQEPLLRVAGPIVQCQILETALLNLINFQTLVATKASRVCLAA
                     DGDPVIEFGLRRAQGIDGGLAAARAAYVGGCVATSNLLAGHLFDIPVKGTHAHSWVMS
                     YPSELEAFQAYAEALPNNCVFLVDTYDTLEGVRNATRAGRWLREHGHEMVGIRLDSGD
                     LAYLSIEARKILDEAGFPEAGITASNDLDEEVITSLK"
BASE COUNT         2866 a         5967 c         5686 g         2802 t
ORIGIN      
        1 ctttgtcatc gattcacgct ggccgagagg ccgaactagt cactggcgac tgactgttga
       61 tgacgattgg ctgcgccgac cgatagggcg ggatctaccc cggtctctga cgccaactat
      121 cagaggaggc gagttcctag aatatgtctc taggcacgcg atgacttcaa aacattggca
      181 tgccttcatc tcagttcccg tcattctagg ggagtatttc ctcggagccc tctcacttga
      241 gacgagacta gagcccatcc aacatcttgt cgcaactaag ctgtctgcaa ggtatgatgc
      301 tatggcgcca aggctcaatt tcggagtcct tcactcggca gcagcagcgg cggcgtacat
      361 gtggctccga gaacgcgata aactcaaccc ccgccatgcc gcaaaactca ttgagtcctt
      421 tgccagcgat tctccagccc cgccaagtcc tacctaccgg cgatcacctg aatccgattg
      481 gatcaagagg cgaacagtgt tgaacccaga gcagctttac gaggccggat gccattttgg
      541 aattttccga aagcgcgcct cgagaataga aatcgtcaac atcacgcccc gcctgctcga
      601 cctcctccgc gagtctccag aacacctcag ccaactgagc cctggacagt tcgaagaact
      661 ggttgctgaa cgattggaca ggatgggttt cgacgttgca ttgactggcg cagctagtag
      721 gcctgacgga ggaattgatt tagttgccac cccaaaggag agaaatgttg caagcttcct
      781 tttggcagca cagataaagc atcactccgc aggaagaccg acaacgcgcg aaagcatcga
      841 tcgtcttctg gcttggaagg attcagtatt ccgactgggc ctggttgtaa ccaacacgag
      901 gtttactgag cacgcaaggt gggtggccat gcaaaatctg gactttctca ggctgcgaga
      961 ctttgatgat ctccgacgat ggttgtacga tgacttttct agcgatgagg actggggtga
     1021 actgccggcg tcagttcagc tagcccccgg tattgtggtg gaaattcctc gtccaagtct
     1081 attaaaaatg cggccgagca ggaggtccta actatctctt gagagtgatt atagctaaag
     1141 tatcctctct ccccagcaag tcggatagga tatcggcgag ttcgcgccgc gacagcacct
     1201 tacctccctg gccgttccgc gaaatctctg tccaattcga ggcacctcaa ctgccgtccg
     1261 ccatgggcgc ggaagcagct acctccttac tcatagtccg cgctgcacgt aaacgatatc
     1321 ggatacaaaa gttcgcttgt tttgtcgtcg agcagcaggg cctcaagagc gatccaggca
     1381 tcattgaggc aatccacgaa atttccccta gctcgaattt gtgtgaatta ctccactggg
     1441 gaggcctctg aaaacttgcg tctcggccct gacaggcagg caagcgtgga actgaccggg
     1501 acgccagctt ctctcgtatt atccattcac cctcacgaag caaaaggctg aaagtgcagc
     1561 tccgggttat gcctcgcctt gcgaagatac tcgagcatcc ttcgggtgaa ctccagtgtc
     1621 gccccctctc tttccaccgc ttgccggaag agggcgaggg cggcgagcgc ttcccggtga
     1681 atgtcttgcg ccttgaagat agggatcatc tgacgggcga gcgtcttcac atcctccgtg
     1741 cgtccctcct gaagatagag gaccgcaagc tccattgaga tcagtgcggt atcaaagccc
     1801 atgccgcgcc tcacgaactc cccacgaacc ttgctaaaga gagtgaccgc attctccgtt
     1861 tcgcccctct cggcagccat acgcgcttcc acccaccgga gtcggattcg gtcgagcgtc
     1921 cctccgcgac accgtgagag gtcgagggcc tttggaaaat attgccttgc ttcctcatgc
     1981 cgccccgcat tcgtgaggag ccagacgagg ttgtggtgca ggatgaggag aagccgctcc
     2041 tcggcagggc cgatgtccgg cgcggcctcg taaaggctcg tgatcgctcc ctgccagtct
     2101 cccgactcct cctgggtctt tgatttcttg aggagcacgc gcgcacgtgc ggcaggattc
     2161 ctcgctatcg cgagagcccg gtcgaggaga tcgagtgcct ctgaaagccg tcgctgcgcc
     2221 cggcgaaggg aagccttgag gtcgagaagt cgcgactcct cgaagagatc cggggcgcct
     2281 tctcctgagg cccatagttc atccgagcgc gcgaatgctt cctcagccgc cttgagcttg
     2341 cccgatacgc ggtgcgcgtt cccgaggtga gccgcagcga acccctggga tctcgcttgg
     2401 agactttccg ggcctgcgac atgctctgcg acaaacccgg caaactcggc caaggcgatc
     2461 gcctccctgg gttcggatga acaaagctct tcgctcctta tcgagagaaa ttcgacgaac
     2521 gcccatgtct tgaactcatc gccgcgcgcc acgacctcct gccagttaga ggaaggatgg
     2581 cgggtaagcc gcacccatag gtacggcgcc ctttcgcggg cagcggcgag ggcaagcact
     2641 gcggctggcg gtaccggttc ctcccggacc cgattcgcgg cctccgtctc ggctgtacgc
     2701 agccgcgaaa ggaacgatac cgcatggtca agtgccgacc atgcgagccc tagagcacca
     2761 agaatccggt ggatcgtcga cgcatccgga accccgcggt cctcctcgct ctcggcgatc
     2821 gccgaccgac tcacccccga ttccttcgcg aggccgatct gggagaagcc gcgtgcatgg
     2881 cgcagcactc gaacgaccgc gccgaggctt gcggtctcct cgccccaggg agcctcctgc
     2941 tcgatttcgc tggactcaag gcttcggagg aacgctgccg catggtcaag cgccgaccac
     3001 ttgaagcgca ttccccgaag aagccgctcg atcgtcgcga catccgggag ggccttgtcg
     3061 ctctcgtact cggagatcgc ggggcgcttc accctggagc gctcggcgag ctcggtctgc
     3121 gagaatcctc gggctccccg cagaacgcgg agcttgccgc cgaatcccag gttcccgcgt
     3181 tccgtccgcc ttcccatccc catcgagtat ctcactcctc cttggtggtg gagcgggcgg
     3241 ggcttgaccg ctcccgccgc acgaactcga gcacctggcg cgccacttcg tcggtgagcg
     3301 tctcggcctc ggccgcctgg aggaaaagcg tggcggcgcc aaggacctcc cgcgtgacgc
     3361 cttggctcgc gaaaagagga agcgcctccc gggccaggat cttcacctct tcgagacgcc
     3421 cctcacggag ccgtagggtg gcgagttcta gtcccacaag cgccgcgtcg tagccgagat
     3481 tttggcttgc gaactcctgt cgcacttcct ccaggatctt caccgcctca tcaaggtgcc
     3541 cgaaaccagc agcgatctgg gcctcgaccc agcgcaatcg aaggagatcg agccggccgc
     3601 ctgccgacgc ggtaagaccg gaaacggaac gaacaagaag gtgggcttca agatggcgtc
     3661 ccgctatggc gaggagccac atgaggttgt gcgtggcgca gagaaggagc cgcggatcgt
     3721 tcgcctcctc gacgagcggg actgcgtctc tcaagacacc gatggcccga tcgacctcgc
     3781 ccacttcctc cagggtcttc gccttcttta tgaggactcg gccccgcatc tctggcgtga
     3841 tctcgggagc agcaagcgct tcctccaaaa gctccaagga ctcgggcagc cggcgttgcg
     3901 cccggcggag ggaggccttg agatcgagga tgcgggcctc ataccccagc acgtcgccgg
     3961 cctgagttcc cccgcgtgac caccagcgat ccgactgaag aaaggactgc tccgccgccc
     4021 tcaggtcacc cgaaacccgc tgcgcgtttc cgaggtacgc gtaggcgttg gcccggagct
     4081 catcgagcca taactgttcc gccggctccc actcctcgag cgccaagctc aagacaaccg
     4141 cgagttccgc aaaatgcaca gccttcttcg ggtcctcggc cgctgctctc tcgctcgcgt
     4201 cggagaggtg ctcgcagagc ccccagctcc tccattgggg atccgactcg atcgtcttcc
     4261 ttctcgcctc gagcggtagt gcttcgagtt ccgcgaagag cgccggagcc tccgcgcgag
     4321 agcgggcaag cccaatggct atcgggcaga gatccaaatc gacggcgccc gcctcgtaga
     4381 ggtcaaggat cgcgccccct gccttgcggc attcaggaca gacggcaatc agatggagca
     4441 gcgctcggct ccatgcctcc ttgtcgaggt ggttctccaa gagatgattc agggcgtcgg
     4501 ggtccagatg gttcgccgtc attgcggata cctccttctc tcgtagtgtc gtgtttcttg
     4561 aagttgtgcc gctcaagggg gtaggtgctg cttcctccgg gacaggggac cggggccgtg
     4621 gtgcagggca aacggtcccg tccgggggac gatctgtgcg gtctgcggga cactcttctt
     4681 ttttgagggc ggcacggcga gaccaatcgt gggcttgcat cggaagctgc ccgaagaaag
     4741 gatcggcccg catgaacacc tcgcgcatcc tttgcgccct tctgctcgcc agtactctcc
     4801 tcctctctgt ggcacctaac gcctccgcct caggaggtct ctctcccgtt ctgtctccgg
     4861 cctcttcccg aatcgcgttc tcctggacag aactgcccgg ccgtatcctc aggctcatcg
     4921 gcagcgcctg ggagaaagag ggggcctccc tcgatccaag cggattacct aagccgggag
     4981 gctccacgcc ccctcctccg gttgcgaccg cctcgggaga ggatgagacc ttgccgctca
     5041 gcctgcgcgc gtcccgctaa tgccgagagg ggcaggagag cgcccgtctc ctgcccctcc
     5101 ttgcctcttt agacgctcgt ccggcacaca ctgtatctga aaggcgtcac gccatggtag
     5161 gctgtgccct cttgcagcta ctatacgtgc gcaggaggcg ccccatgcag ggaggaatgt
     5221 ttcgaaccct tggcacgact ctcgtactct tgcgtcgcct tcggcggcgg agccaggcat
     5281 ggctcgctcg gcgtgcgggt atgcggcccg agcggctctc ggcctacgag agcggacaga
     5341 gcctgccgca gcttgagacc ctggagcgga tcttcgaggc tctcgaggtg gagccccacg
     5401 aatttttcac gctcgtgcgt ttcaccgact cgctcgagga gcgagagagg cccgcctcta
     5461 ccgggatctc agacgaaccg cgttcacccc gctctcgccc cgatgcgggc tacgcattcg
     5521 atgtcttcct cctcttcctc caaggcacgc acgtcacacc ctctccatca gcttcttcaa
     5581 agaccccgca gagaggggcc cggcggatca gccgataggc ccttgcaggc aggagagccg
     5641 agcgccggtc tcccgctaac ggcattccgt accggagagg ggacatcctt ctctcgcgga
     5701 cgcacggcgg ctaggctcac gtgccgtgcg ctatacgaga gacggtgtcc cagatctcgg
     5761 gcaggtcagg ggcgaagaga tggcgaagcg tgcccttgag gtcgccttgg ccggcggaca
     5821 ccacttcctc ctcaagggtc ttcccggatg cggcaagagc ctgcttctgc ggtgcattcc
     5881 gggacttcta cctcctctta gccccggcga ggtgcgggaa ctggcggacc tcggaagagg
     5941 aggcgctcgg cccttccgcc ccgtccgctc gccagcccca ggtatcaccc cgcaccgcat
     6001 ggcagggtgg agccgccgga gccccggcga ggcgatctcc gcgcatggcg gggtcctcgt
     6061 cctcgatggc ctgcacgcct tccgccgcga cacccttctc tcgcttcttc ggcccatgga
     6121 cgaggggacg gtcgaagcga ggggagagcg gctacccgcc cgcttccagg tcgtaggcgc
     6181 tctcgctccc gcggggcgga agaggctgcc gcctgacctc ctcgaccgga tggatcttga
     6241 agtcgtgatg ggagcgcagg ccccacccga cctctgggct cctcgggagg aggacgggac
     6301 ggcggaagcg gcgaagcgaa tcgcgggcgc gagggcgatc caggccgagc gcttcgagcc
     6361 tcaccctgcc aggataaacg cccggatgag cgaggacgac atcctctggt actgcgttct
     6421 tgaccgggcc tcgaagagcc ttcttgaaga ggcccgagca cgatttcacc tctcggcccg
     6481 gagcctcctc agtattcaca aggtggcccg cacgatcgct gatcttgccg gctccgatgt
     6541 gattcgcgcc ccgcatctcg ccgaggcact ccagtaccgg aagctccgga cgaggaaggc
     6601 tatgggcgaa gagacacaag aagggagaga gtgagccccg tgaagcttgg agagatccgg
     6661 cgggctatag agaaagagac tgaactcctc agcgagatcg ccgagcgcct ccggaacttc
     6721 gctgcgcggg tcgaagcccc gactgcggag gaactccggg cgatcgaggc cggggaggcc
     6781 ccgatgaccg cggaggcgca tctcataagc gtgcttacgg agcaggccct ctcggtcggc
     6841 gaggcggccg tcgccttacg agacacgagg catgcgacca ccgacctttt ccggaaagcg
     6901 tgggctccgg gctctcgtcc ctttgcaagg gcgatagccc atctccgagc gggtctccgg
     6961 ggccgaacgc tccctgcgga ctttaggccg gaggagcctc tcggcacgca cccctggccc
     7021 ccgccggcgg gcagcgcgct cgtcgccgac ctcctcgcca agggttaccg ctgggagttc
     7081 gaccttccgc ccttgcccca acgggagcgg aagaagtcgt aacgagtcac gccaactctg
     7141 ggaaggcaca tggcggcaga gggctccatc ttgcgtgagg ggctcctccg gctccggcag
     7201 cgcgcggccc ttgaggtcga ggcgctcaaa gcgaggcgga tgcggggcga ggtggacccg
     7261 tgggacgggg acttcctacg cgacctcacg ctcgcatatc ggggagcggg ggacgaggtt
     7321 ggcgcccgca tcacatatcc ccaggcatgc acctccgccc gcaggcggaa ggacgatggc
     7381 ctcctcctat cgcttgtccg gatgactcgc ctcttcctga atccggattc cgccgagtcg
     7441 catcttcgtt gggcgaggga acttgcccgg gagaagggct tctgcggaga ggaggcgcgc
     7501 tgcctgaacg acttgggagt cgtcgccctg gagcttcgga acttccccct agcccatgag
     7561 cggctcctcg cctctcttgg cgtctttgga gccctgggag aaaacgagga gaccgccgtg
     7621 cccctcatga acctcgcctg tctcgggttc gcgcggggag atcttgcaga ggcccgggag
     7681 cgcttggcgg tagccagggt gtgcgcggca gggaacctct cgcgcgtgct cgcgatccgt
     7741 ctcctccagg caatcctccg ggcggtggaa gagaaggact tccttggctg tctcgacgag
     7801 cttcaagaga tcgccaaaga ggctccggcc tctgacgacc tggaaacgct cttggccctt
     7861 ggctttgcct taggccgggc gtacctcctc cgaggaaacc cgcgctccgc cttggacgtg
     7921 gcgcttcctc cccctcgctg ggggaatgac gatgcgcttg cttatggcgc ccttgctgcc
     7981 ctttcactcg aggcgtggga aaccatggac gtggccccac tcccggagac actggaggag
     8041 gctgaggctc ttagtcggac gacctctctc caggcatggc ggtaccggca gacgtgggta
     8101 ccccgtctcg ttctctcaat tgatatacct gtgaggccgt aaggctaaag gtcagctccg
     8161 catccgctga cagcttatcc tttcttcaag agtcgtcgcg ctctggctgg ccgtctcccc
     8221 aacgtactcc tcgaagagac gaacgagacg cgggtaacgc ttcttgacct gctcggtctc
     8281 ccaagccacc aaggttgagg ggtcgagccc aagacggcgg gcaagctccc gttgggaaag
     8341 accttgccga cgccgggctg ccttgatcct ctcgccaagc cctgaagttg gatcgggagc
     8401 cacagggtcg tatcccagga agccgatgac cgccggcaga aagcggatct ccggttcgtg
     8461 gaggccctgc tcccacttct ccagtgttgc cttcgagacg ccgagctgtc ccgctacctc
     8521 cttctgcccg agtccgaggt caagccgcct cttcttgagg tgctccctca gggtccgggg
     8581 gctttcaggg taaccaggtc ggagtttctg tccccggagg gtgacatggc aacttatgaa
     8641 tgaattcctg tccctgcggc catctgggag atccgcggca cgactgcaga tgtccgctgc
     8701 ctctcgtcga acgctaccgc agccgcgtct cgggtccgct gctggaccgg atcgacctgc
     8761 acgtggaggt gccggcggtg agcctgaaag agctgcggtc cgacccgggc gagagcacgg
     8821 ccgacgtggc cgcccgcgtc gcccaggccc gggccgtcca gctcgagcgc ttcgggacgg
     8881 gctcttcgac tccggtcaac gcggtgatgg ggcccgaggc cctccgccgg cactgccggg
     8941 tcgatcccgc gggccggacc ctgctcgact ccgccttcga gaagctcggc ctctccgctc
     9001 gcgccctgga ccgggtcctc cgcgtcgccc gcaccatcgc cgacctcgcc ggcagcgata
     9061 ccatccgctc tgcgcacctg gcggaggcga tccagtaccg gagcctggac aggcgggtga
     9121 cctggtgagg cccgggagag acgtccgcca cagcctcata ccttattggc atacgcgtat
     9181 aagcaggcaa ctcctcccag gtagcggagg gccccggctt ggcaagggct gcaaaacgcc
     9241 tctcgcggga gagggcggac catgtagaaa agttaagctc catgctggac cgccatattt
     9301 tattggcctc ttgacaagct cctttcggcc gctacaatac gcaggcacca gaggtttcgc
     9361 cctttcgagt caaggtttgt cagggagtcg gcaggtgctc taccgctccc ctgctggtgg
     9421 tacaagcatg tccaaacggc accatacggt catcttcgtc cctcacgcgc acaccaagct
     9481 acggaagtgg cgcatcagca atctccagat cggggtggcg gtcggggctc tggtcctgct
     9541 gacggtcgcc gccgctctag ctctctggtc gttcttctcg accccctcca atcccgccga
     9601 ggtcgcccgc ctgcgccagg aaaacgagca gctccggctg gcgaaccacc gcttcgagac
     9661 gagcatccag tcgcttcaga accagctcgg ctcctatgag gagcgcacgc ggcagctcgc
     9721 catcgtggcc ggcctcgaga atctcggcga gggcgtggag gccggcatcg gcggcggtga
     9781 gcccatgcct ccgatcgcgg atttgggggc cggcggaccg gctcccggcg aagaagcccg
     9841 cggcgagatc gacgccaccg atctccctga gatggcgagc cgcgccgagc atctggcggg
     9901 cacgctcgac caggtgcagg ccaagctcga cgagcggatg cgctggctct ccgccacgcc
     9961 cgcgatcact ccggtcaggg ggatcctcac cagcggcttc gggtatcgct ccgacccggt
    10021 gacccacggc cgcggcgtcc accagggcgt cgacatcgcc gccgccaccg gccagccggt
    10081 caaggcctcg gcggacggca tcgtcatccg cgccggcgtc gagggcggtc tcggccaggc
    10141 cgtcttcctc tcccacggct acgggctcac cacccgctac ggccacctgt cgcggatcga
    10201 cgtccgcccc ggtcagcggg tcaagcgcgg cgaggtcgtc ggccgcgtcg gcaacaccgg
    10261 ccgctccacc ggcccccatc tccactacga ggtgcgcgtc gatggcgagc cggtcaatcc
    10321 tctggcctac ctctacgacc acggcgccgg cggctcctag accgctccgc cttctcgacc
    10381 tttctggtgg ctcttcctct ctatagggga tgggcttgcc ccccctcccg ggaggggggg
    10441 caagcccatc ccctacatca cttcatacca ccggcagggt gattgtcact tcgaggccgc
    10501 cttcggggcg gttgcgggcg ataatgccgc cgccgtgctc ctcgacgata cggcgggcga
    10561 tgggcaggcc gaggccggtg cccgtgtcgt gggtcgagaa gtagggatcg aagatccgct
    10621 gcagattctc ggggcggacg cctggaccgg agtccaggac ggcgatgcgg gcgatgccgt
    10681 cgtttcgctc cacccgcacc accacctcgc cgctcctgcc ggtgctcgcg ccggcggccc
    10741 gcagggcgtt ctccatcagg ttgcgcaccg cccgccccag gagcttggcg tcgaagcggg
    10801 cggggatggc gtcgttggtc tcgagctcca ccctcactcc ttcgggtgga gccgcctggt
    10861 agccctccac cagctcggcc accgccgccg tcaggtcgcc cggacggggg gcgatgcgcg
    10921 ggatggcgct gtaggccgag aactccgccg agatcgagcg cagctcctcc acctgggtga
    10981 ggatgttgga cgtgcagcgg tcgaacaccc ggtcgaagtg ggaggggtcg ctgcggtaga
    11041 cctcccgcat gtgctcggcc gagaggcgga tgggggtcag cgggttcttg atctcgtgcg
    11101 cgatcatgcg ggccatttcg gcccaggcga ggagccgctg gccgcgcagc acctcggtcg
    11161 cgtcctccac caccagcagg gcgctcggct cgcccgagcc ggggaccggc acccagacga
    11221 ggctccactc ccgctcgccg ccggcgtcgg cgggcaggcg cacggtctcg cgcgccatct
    11281 cggaagcgac cgtgcccagg aaggcggaga ccccgccgag acgctcgccg cgcgccaccg
    11341 cctcttccag gttctccccc acctccaccc ccagcagctc ggccgccacc cggttgcgca
    11401 tcagcacccg gcggtcgcgg tcgacggaga ccacccccga ggtgatgttg tcgaccatgc
    11461 gctccaccac ctgcttctcg cgcatcagcc gctcccgccc ttcggcgatg cggcgggcca
    11521 tctcgtccac cgccgcgacc agcgaggcca gctccaggtc ggagggggcg aggtcgaggg
    11581 acggggcacc cgcggcgata cggcgggtcc cctcgatgag ctgcatcagc ggccgggtga
    11641 agttgcgggc caggcgcata ccgaccgcgg cgagcaggat gaacaggccg gtcgccacca
    11701 gcacgccctg gcgccgcatc tgggcgaggt cgcgcgccgc ctcctcctgc tgggccagca
    11761 gcgggatgga caggaacagc cgaccctcgc ccggggcctc gttgcccacc cgcaccggcg
    11821 cgtagagctc caggtaggtg gtgtcccccg cgacgttggt gcgcgaggag agggcgaagc
    11881 ctcccagcac gaggtgggag tagatctcgc cggggatgcg ccggggcagc aagccggccg
    11941 tgaacagctc gtgcttggtc gaggcgtaga ccccactccc ccagtagagg ttcacctcgt
    12001 gatggatcac ttcggagatc cagctcagca ggtcgtcgtc cagctcggtc tccaggatga
    12061 accccggcgg cagaagcagg atgtactcgc ccagcacctt ctgcgccgac cacatggccg
    12121 cttcgcccgc cgcccgctgc tcttcctgca gccgctgctc catggcctgc accaggaagg
    12181 cggagaggag gagcagcggc agcagcagga gagccgtgta gaccaccagc aggcgcttgg
    12241 agtacgagcg catcacccgc tgcaccccgt cgcggaacgc ggaccggggc aggctcggca
    12301 gcagcaggag ggggaggacc gtggcgagca ggaacagggt gccgatcccc gcgttggcga
    12361 tccgctccag tgatcgcagc ggggtgagca gcggcaggta gatcacctcc caaccctgcc
    12421 gcgtggcacg gctgaaggcg tagaccgggc cggacggggt gctcaccggc ccgatacgga
    12481 cgaccttccg cggctcccgc agcaggcgcg gatcgaggga aggagactcc tcccagggcg
    12541 agagtgccac ccggccgtca gcccagtaga gggcgaagag ggcggggccg gtgatgtcct
    12601 ccacctggga ggcggccggc ccccccttga ggagacccgc ttcgacgtcc tccagccggt
    12661 cgccgcgcag ttggaaccct ggtcgcggca tcagccagta gcgcaccgtc ccccaagggc
    12721 ggccggcgaa acgcagctcc gcctgtcccg ggatcagagt ctcctcccag agcggcagcc
    12781 ccagatcctg ccaggagggc gagttggtgt cgatgccccc ctctgcggcc agcgggatgc
    12841 cgaacgagaa ggaggacggg acgccttcca gcggctccac caccaccgcc gacagcgcgt
    12901 gcgggcgcgc caggggggag tcgcgccaga gggcgaaggc caggtcctgc cgctccaagc
    12961 cggcgtcggc gcgcggcacc agatccctca ggtcgtagga ctggaagtgg gcccggaggc
    13021 ctttctggag ctccgagatc gcctgctcgg acggtggcgc gagccggtcc agcagcccgg
    13081 gcccggcctc ggcgcgcaga cggagccggt agccgacctc ccagctcccc atcgccacca
    13141 gcacggaaaa cagggtgagg acccccagcc cgatgccctg gcccagccgg cggtgccgcg
    13201 cgtagaacgc ggtgaccgcg ccgccggcgg cgagcagcgg caaggcggcc caagggtggt
    13261 tggagagcat gcctccggcc agcagcagca gcacggcgag ccaggcccag cggtcccgcg
    13321 gccgggtcgg cgccggcagg gtgaggcccc ggaagcagag caggccgaag gccgcggcgg
    13381 tccacgcgag cctcagggcg tgggccacgg gcggcaccag gatgctcgag gacaggtcca
    13441 gcggaccccg cagccactgg agcccacagg ccgcggcggc gagcagcagc acggtgacca
    13501 ctccccatac cgggatccac cgggcccccc agggagggcc cgccctcccc gcgaagtggc
    13561 ggccggtcag ccccagggcc gcgatcagga gcccggcttc gagagcgatc agcgcgaaga
    13621 ggggcagcgc cgccgccatc cccgcggcga aggcaccgcc gaggacgttg gccgcgatcc
    13681 agaaccggcg ctcgcggatt tttccggtcg ggaacacgcc cggcgcgagg atcagcccga
    13741 gcgcccgcgt cgccgcttgc gacagcaggg cgaaggcgat ggccgcccac gccacccggc
    13801 gtgcccacgg ccgttccgcc ggcgccccgg gagccccttc caaggcggga tcgatgacca
    13861 gggtcggggc tccttcggca cgcacctgga tcgcgccggg cagcgcctgg gcgggatcgt
    13921 ccaccaccga ccagcgctgc ggccggtcga gacccgggaa cggcaggacg tgggtgggga
    13981 agctggcgcc ggcgacgatc cgccagggcc gccgcgcctc gtccaggggg gcgacggcaa
    14041 gaaacgtcac ggcgctccag ccgaccgcgc tgtgcaggcc gtcgcggggc aggctgtccg
    14101 gccggggctc gtggagcaac ccctcccccg cccaggcgat ggcggtgccg tccgggtcga
    14161 gcagcagcag ggcccggcgc tcccgcccgc gggtgggctg gagcgcggcc agccggctga
    14221 acgcggccag gcgctcctcc cgcgtctcgg gcggccggcc gacggccgcc accgccctct
    14281 gcgcctcgtc ccgcagtcca tcccacagct cggcgtactc ccgccgcgcc tcccccaccc
    14341 aatcgcgcga tgacgagcgg gcctgccgct cctccagcca tcccgccacc agcgccgtgg
    14401 ccgacaccgc cagcgcgatg gtgaccacca ccacctcgcg gcggggcggg cgcgtccaga
    14461 gccagaggac ggtgaggagc gccagggcca gcgccggcca cggcagcggc aggaagggcg
    14521 ccgagacggt acccaggagc agggcgaggt tgaggattcg ggatctcaag ggttgttgga
    14581 gccccgcaat gggacggggc tcacccctgg actaaaccgc acggatcact ccccgcttcc
    14641 cgttgctccc ggccggccga gcatggggat gtcgacgccg tgccggcggc cgaactcgat
    14701 cgcctcggga tagccggcgt cggcgtggcg gacgaccccc atgccgggat cgccggtgag
    14761 cacccgctcg agccgcgccg ccgccgcctc ggtgccgtcg gcgacgatca ccatgccggc
    14821 gtgcagcgag tagccgatgc cgacgccgcc gccgtggtgg accgacaccc aggtggcgcc
    14881 gttgaccgcg ttgagcatcg cgttgagcac cggccagtcg ccgatggcgt cggagccgtc
    14941 gagcatcccc tcggtctcgc ggttgggcga ggcgaccgag ccgcagtcca gatggtcgcg
    15001 gccgatgacg atgggcgcct tgacccggcc cgacgccacc agctcgttga acacccggcc
    15061 ggcccgcgcc cgctcgccgt agccgagcca gcagatgcgc gccggcagcc cctggaaggc
    15121 cacccgctcc cgggccaacc ggatccagcg cgccagcgcc tggtcctcgg ggaaggtggc
    15181 gaggatcgcc tcgtcggtga ccgccaggtc ttcgggatcg ccggagagga ccgcccagcg
    15241 gaacgggccc ttgccctcgc agaacagcgg ccggatgaac aggggcacga agccgccgat
    15301 cttgaaggcg cggtccacgc cagcctggag cgcctggccg cgcaggttgt tgccgtagtc
    15361 gaaggtgacc gccccccgct cctggagcac cagcatggcg cgcacgtggg cggccatcga
    15421 ctccatggag cgggcgatgt aggtctccgg gtcctcctgg cgcaggcgca gcgcctcgtc
    15481 gtagcgcagg ccgtggggga cgtagccctg gagcgcgtca tgagcggagg tctggtcggt
    15541 cagcgcgtcg gggacgaggc cggcggccac caggtgctcg aggagctcga cggcattggc
    15601 ctcgacggcg atggacacgg cctggcgccg gtcgcgggcg gcgagggcac gctcgacgcc
    15661 ggtgcggatg tccggggcga cctcatccag ataacgggtg gcgagccgcc gctcgatgcg
    15721 gctgcggtcc acctcggcga ccaggcagac cccctcgttc atggtgacgg ccagcggctg
    15781 ggcgccgccc atgccgccga ggccggcggt gacggtgagg cgtccggcga ggctcccccc
    15841 gaagtgctgg cgggcgcact cggcgagggt ctcgtaggtc ccctggagga tgccctgcgt
    15901 gccgatgtag atccacgagc cggcggtcat ctggccgtac atggtgaggc cgagcccttc
    15961 caggcgccgg aactcgtcgg ccgttgccca acgggggacg agcatggagt tggccaggag
    16021 cacgcgggga gcttccgtat gggtccggaa gaccgccacc ggcttgccgg actggacgag
    16081 cagggtctcg tcgtcgccca gccgcttcag cgtagcgacg atggcctcga agcactccca
    16141 gttgcgcgcc gccttgcccg agccgccgta gaccaccagc tcctccggcc gctcggcgac
    16201 ctccgggtcg agattgttca tcagcatccg cagcgccgcc tcctgctgcc agccgcggca
    16261 ggagatctcg gtgccgcggg gggcgcggac ggtacgggga gaggcggcct cgggcctcgg
    16321 ctcggtggcg atggtggtgg tcatgggcgt attgtatccg cgccacgcga taggaggatg
    16381 ccatggggac gctgacccgc ctctatgggg agcgcctggc gctcctcacc gacctctacc
    16441 agatcaccat ggcctatgcc tactggaagt cggggacctt ccggaaggaa gcggccttcc
    16501 atctccacta ccggaagaac ccgttcggcg gcggctatgc ggtggcgtgc gggctcgagc
    16561 cggtcctcga cttcctggag gggttccgct tcggcgacga ggagctgggc tacctcgccg
    16621 gcctccgggg caacgacggc gcgccgctct tcgagaggag cttcctcgac tacctgcggg
    16681 agatgacgtt ccagtgcgac gtcgacgcca tcccggaggg gacggtggtc tttccccagg
    16741 agccgctgtt gcgggtggcc ggaccgatcg tccagtgcca gatcctcgag accgcgctcc
    16801 tcaacctgat caacttccag acgctcgtcg ccaccaaggc ctcgcgggtc tgcctggcgg
    16861 ccgacggcga tccggtgatc gagttcgggc tgcgccgtgc gcaggggatc gatggcggac
    16921 tggccgccgc ccgtgccgcc tacgtcggcg gctgcgtcgc cacctcgaac ctgctggccg
    16981 gccatctctt cgacattccg gtcaagggga cgcatgccca tagctgggtg atgtcctacc
    17041 cctccgagct ggaggcattc caggcctacg ccgaggccct gcccaacaac tgcgtcttcc
    17101 tggtggatac ctacgacact ctggaggggg tgcgcaacgc cacccgcgcc ggccgctggc
    17161 tgcgcgagca cggccacgag atggtgggca tccgcctcga ctcgggcgac ctcgcctatc
    17221 tgagcatcga ggcccgcaag atcctcgacg aggccggctt ccccgaggcg ggcatcaccg
    17281 ccagcaacga cctggacgag gaggtcatca ccagcctgaa g
//
LOCUS       DASPJI010000811         7446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_729837, whole genome shotgun sequence.
ACCESSION   DASPJI010000811 DASPJI010000000
VERSION     DASPJI010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7446)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729837"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..>7446
                     /locus_tag="VEL74_22885"
     CDS             61..>7446
                     /locus_tag="VEL74_22885"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MupA/Atu3671 family FMN-dependent
                     luciferase-like monooxygenase"
                     /protein_id="HYG65446.1"
                     /translation="MTPVGSDPVHQLLAQQLQLMTYQVDLLRQRGGAATYPVAEPPRP
                     AQLPQAVPAAAAAEPVRPVASAPPAPVQAVAPAPAQVPVSPAAPAAARPGAEAFGPFQ
                     PLEVGLGDRLTPHQQEALTTFIEEYTSRTRESKRMTQQYRPYLADTRGTVGFRMLWKE
                     MIYPIAGSRSHGSRVWDVDGNEYIDISLGFGLHPFGHSPDFIDHALREQLDLGIAVGP
                     QSALAGEVAELFCEVTGVERAMFCNSGTEAVMGAIRAARVVTRRNRIAVFTGAYHGWA
                     DTTLVRSVTRDGVRESAPLASGVSPLAVQDALVLDYGKPEALETIRAHGRELAAVLVE
                     PVQSRRPEFQPREFLHELRRITEENGIALIFDEMITGLRIHPGGAQSWFGVRADLATY
                     GKLLAGGLPIGVVAGRAPFMDVLDGGMWSFGDSSYPQAEKIFFAGAFFKHPLTMAAMR
                     AILLHMKEKGPRLQEELNRRTTWLVDTLNAYMRENQVPVQVVHFGSLFRFRLANDLKF
                     KELFGHQLLLRGVFYSPESGNCFLSTAHSQEDLDYVIRAVKESVQAMRRAQFWPSQPD
                     APTGGGPGGGEKARGERPAVTVTATASAPAGAVALNATPRAVSSRAVGMQFGMFFFGS
                     YDPGDRDKYELVIEGARFADRHGFSAVSVPERHFHPLGGFSPNPSVVAAALARETERL
                     QLRAGSVVVPLHHPIRIAEEWAMVDNLSGGRVGICFASGWHPDDFVFAPDSYDKRRVL
                     MTEGIETVRSLWRGETVRTRSGTGQDLDVKLYPMPIQKELQIWLTAAHAETYRKAGEI
                     GAGVLSNLLGHSMDELRQRIKLYRDTLAEHGDPRSGHVTMLVHSFLDETVEKARSRAR
                     QGFHGYIRSSFHLVSNQIERSGQGDALARLSDDDRELLLANTADRYLRSCTLIGTPDS
                     CAELVETLSDMGVDEIGCFIDFGVDGGAVLESLDALRELKDRFQERLRPTADELSPVI
                     SLASRVRGGAETRPQEITAPATEGQKGLWILAQMGPDASRAYNESFVLEMRGPFEREV
                     MRRALDRIVARHDALRTTFSEDGSLQRIAAHTDLELPLLDLSALPTDERTAAFTAWKR
                     QQVCDVFDLLNGPTLRLSLVRLGPEHHVLAFTTHHIVTDSRSSGVILRELSSLYAAER
                     EGRDLELPPATQFREHIARRTPEEAWQERERAEAFWQTQFQDGVPALELPTDRPRPAV
                     QTFAGSREHGHLEGRLCDRLRRLASRQGSTFYMTLVAGFQLLLHKLSGQESVVVGADA
                     IDPSVRAGAMVGYTINPLPLRSNLNGDPRFTDFLKGVKRSLLEGFAYQGYSLTGLVQK
                     LQAEHRDHSRPALFAASINLEGGGTLSLPGLEVDIAPNLPPASRFDLGLNHIERGAEI
                     EVVCEYNTDLFDATTIRRWLDDYRALLTAIADHADQPVSVLHALAEGERHQTLVEWND
                     SAREGFAAHTFQRLFEIQVKSTPRARAALCEGEAVTYRELNARANHCAALLAGQGAGP
                     ETRVALVADRGIDFLVAILGVFKAGAAYLPIEPRDPTPRQAQILARSGASLVLVSREL
                     APALAEVAESLPAGARPRLLVMEEACRGEGGQTNPEPRQQGNGLAYVIYTSGSTGMPK
                     GAMIEQRGMLNHLLAKVTDLDLTATDRVAQTASQCFDISVWQFLVPLIVGGSVHIVPR
                     EVVQDPVRLLPALEEAGITVFETVPSLLRLLVAEAAGRGPARPPLPALRWLLSTGEAL
                     PPDLCDEWHRHCPGVPLVNAYGPTECSDDVTHQVLRAAPGRRSDRIPVGRPIVNTQLY
                     VVGRSLEPLPMGVAGELCVGGFGVGRGYLGEPARTAEVFVPDPFAEAPGSRLYRTGDL
                     AACQPDGATDFRGRRDHQIKVRGFRIELGDIETALERHPAVAETVVVNRRLGEGDTRL
                     VAYVVAAQDRRPDAAELRAFTKGELPDYMVPSAFVFLDALPLTPNGKIDRKALPAPEP
                     AEGETGLTATRNPVEDLVAGIWAQGLGTEQVNAHDDFFSLGGHSLLATQILARVRQAF
                     QVDLSLRALFEAPTLSGFAARVEEAMKQARGVAAPPLRRVPGGAGGERMPLSFSQQRL
                     WFLDQLQPGSPAYNLPSALRLQGPLDIAALGAALDEILRRHESLRTRFEAAGGEPFQV
                     IQEAEPLPLPLLDLSALPQVSREHEVRRLASEEAKRPFDLARGPFLRVTLARLAPEEH
                     TVLFTTHHVASDAWSMGVLIREVGALFSAFAEGRPSPLSQLALQYSDFAYWQRNWLRD
                     ETLEAQLAYWRQRLAKVRTPINLTVDKARPAVQTFRGASLVFSLSAELGRKAKALARQ
                     QGVTLFMVLLAAYQTLLSHWSDSKDILVGSPIAYRNHVELEELIGFFSNVLVLRTELS
                     GDPTFNELLARVREVTLGAYSHQDVPLEKLVEELRIERNLAYNPLVQVAFTLDNTPQE
                     NLGISDLALSPLDVAVETTPWDLTLNVVDGGRDLAATLQY"
BASE COUNT         1116 a         2549 c         2548 g         1233 t
ORIGIN      
        1 ccgatggtcc agatggtctc tgccgtgccc cagcctgctg cctggaacgc gaccgccccc
       61 gtgacgcccg tgggaagtga tccggtgcac cagctcctgg cgcagcagct ccagctcatg
      121 acctaccagg tcgacctgct ccgacaaagg ggaggggctg ctacgtaccc cgtcgcggag
      181 ccgccccgcc cggcccagct cccgcaggcc gtccccgcgg ccgccgcggc ggagccggtc
      241 cggcctgtgg cgtccgctcc gcccgcaccg gtgcaggcgg tggcgccggc tcctgcccag
      301 gtgccggtct ccccggcggc tcctgccgcc gcgcggcctg gggccgaagc cttcggcccg
      361 ttccagccgc tggaagtggg gctgggggat cggctgacac ctcaccagca ggaggccctg
      421 accacgttca tcgaggagta cacgagccgg acgcgggagt cgaagcggat gacccagcag
      481 taccgtccgt acctcgcgga tacgcgggga acggtgggtt tccggatgct ctggaaagag
      541 atgatctatc ccatcgcggg cagccgctcc catggctccc gggtctggga cgtcgacggg
      601 aatgagtaca tcgatatctc tttggggttt ggcctgcatc cgttcgggca ctcaccggac
      661 ttcatcgatc acgccctgcg cgagcaactc gacctgggca tcgccgtcgg cccgcaatcg
      721 gctctggccg gagaggtggc ggagctgttc tgcgaggtga cgggggtcga gcgggccatg
      781 ttctgcaact ccggcaccga ggcggtcatg ggcgccatcc gggcggcccg cgtggtcacg
      841 cgccgtaaca ggatcgcagt gttcacgggt gcctaccatg gttgggcgga taccactctg
      901 gtccggtcgg tgacccggga cggcgtgcgc gagtcggcgc cgctggcctc cggcgtctcg
      961 ccgctcgcgg tccaggatgc cctggtgctg gactacggca agcccgaggc tctggagacc
     1021 atccgggcgc acggccgcga gctcgctgcg gtgctggtgg aaccggtcca gagccggcgc
     1081 cccgagttcc agccacggga gttcctgcac gagctgcgcc gcatcacgga ggagaacggc
     1141 atcgccctga tcttcgacga gatgatcaca ggcttgcgca tccatcccgg cggcgcccag
     1201 tcgtggttcg gcgtgcgcgc cgacctggcg acgtacggca agctgctcgc cggcgggctg
     1261 cccatcggcg tcgtcgccgg ccgggccccc ttcatggacg tcttggacgg cgggatgtgg
     1321 agcttcggag acagctccta tccgcaggcg gagaagatct tcttcgccgg agccttcttc
     1381 aagcatccgc tgaccatggc ggcgatgagg gcgatcctgc tccacatgaa ggagaagggg
     1441 ccgcgcctcc aggaggagct caaccgccgg accacctggc ttgtcgacac gttgaacgcc
     1501 tacatgcggg agaaccaagt tccggtccag gtggtccact tcgggtccct gttccgcttc
     1561 cggctggcca acgatctcaa gttcaaggag ctcttcggcc accaactcct gctccgcggc
     1621 gtcttttact cgccagagtc gggcaactgc ttcctgagca cggcccacag ccaagaggat
     1681 ctggactatg tcatccgcgc cgtcaaggaa agtgtccagg ccatgcgacg ggcgcagttc
     1741 tggcccagcc aaccggacgc ccccacgggt ggcgggcccg gcggtggcga aaaggcgcgg
     1801 ggcgagcgcc ccgcggtcac cgtcacggcg accgcgagcg ctccggccgg cgccgtggcg
     1861 ctgaacgcca cgccgcgcgc ggtgtcctcc cgtgcggtgg ggatgcagtt cggcatgttc
     1921 ttcttcggga gctacgatcc gggcgatcgt gacaagtacg agctggtgat cgaaggcgca
     1981 cgtttcgccg accgccatgg tttctccgcg gtcagcgtgc cggagcggca tttccacccg
     2041 ctgggcggct tctcgcccaa tccctcggtc gtcgcggcgg cgctcgcccg tgagaccgag
     2101 cgcctgcagc tccgggccgg cagcgtcgtc gttccgctgc atcatcccat ccgcatcgcc
     2161 gaggaatggg cgatggtgga caacctgtcc ggagggcggg taggcatctg cttcgcctcg
     2221 gggtggcacc ctgacgactt cgtgttcgca ccggattcct atgacaagcg gcgggtgctg
     2281 atgaccgagg ggatcgagac ggtgcgcagc ctctggcgtg gagagacggt gcgaacgcgc
     2341 agcggcacgg gccaggatct ggacgtgaag ctctacccca tgccgatcca gaaggagctc
     2401 cagatctggc tcaccgcggc tcatgccgag acctatcgca aggccgggga gatcggcgcg
     2461 ggggtcctta gcaatctcct gggccactcc atggatgagc tgcgacagag aatcaagctg
     2521 tatcgcgaca cactggcgga gcatggggat ccgcggtcgg ggcacgtcac catgctcgtg
     2581 cacagcttcc tcgacgagac cgttgagaaa gccagaagcc gcgcccggca ggggttccac
     2641 ggctacattc ggtcgtcctt ccacttggtc agcaaccaga tcgagaggtc gggacagggc
     2701 gatgccctgg cccggctctc ggacgacgat cgggagctcc tgctggccaa caccgccgac
     2761 cgctacctgc gttcgtgcac ccttatcggc acgccggact cctgcgccga gctggtggag
     2821 acgctgagcg acatgggggt cgacgagatt ggctgtttca tcgatttcgg cgtcgacggt
     2881 ggagcggtgc tggagagcct ggatgcgttg cgggagctca aggaccgatt tcaggagagg
     2941 ctgcggccca ccgctgacga gctgtccccg gtcatctctc tggccagccg tgttcgtggc
     3001 ggtgcggaga cccggccgca ggagatcacc gctcccgcca cggaagggca gaagggtctc
     3061 tggatcctgg ctcagatggg gcccgatgcc tcgcgagcct acaacgagtc gttcgtgctg
     3121 gagatgcgtg ggcctttcga gcgcgaggtg atgcgacggg ccctcgaccg catcgtcgcc
     3181 cgtcatgatg ccctgcgcac gaccttcagc gaggatggct ccctccagcg gatcgcggcc
     3241 cacaccgatc tggagctgcc tcttctcgac ctatcggccc tgccgacgga cgagcggacc
     3301 gccgcgttca ccgcctggaa acgacagcag gtctgcgacg tcttcgacct gctcaacggc
     3361 cccaccctgc gcctgagcct cgtccggctc gggccggagc accacgtcct ggcgttcacc
     3421 acccatcaca ttgtcaccga cagccggtcg agtggcgtca tcctgcggga gctctcgagc
     3481 ctctatgccg cggagcgtga ggggcgggat ctcgagcttc cgccggccac gcagttccgg
     3541 gagcatatcg cccgccgtac ccccgaagag gcctggcagg agagggagcg ggccgaggct
     3601 ttctggcaga cccagttcca ggacggtgtg ccggctctcg agctgcccac cgaccggccg
     3661 cggccagccg tccagacgtt cgccggctcc cgcgagcacg ggcacctgga gggcaggctc
     3721 tgtgatcgtc tgagacggct cgcctcccgc cagggttcca cgttctacat gaccctggtg
     3781 gcgggcttcc agctcctgct gcacaagctc agcggacagg aatccgtggt ggtgggcgcg
     3841 gacgccatcg atccgtctgt ccgtgccggg gcgatggtgg ggtacaccat caacccgtta
     3901 ccgctgcgca gtaatctcaa cggggacccg cggttcacgg atttcctgaa gggagtcaag
     3961 cgtagcctgc tggagggctt cgcgtatcag ggctactcgc tcaccggcct ggttcagaag
     4021 cttcaggccg agcaccgcga ccacagccgt ccggctctgt tcgcggcgtc catcaacctc
     4081 gaagggggag ggacgctgag cctgccgggg ctggaggtgg acatcgctcc gaacctgccg
     4141 ccggcgtcgc gcttcgacct cggtctcaac cacatcgaga gaggcgccga gatcgaggtc
     4201 gtgtgtgagt acaacaccga cctgttcgat gccacgacga tccgccgctg gttggacgac
     4261 taccgggccc tcctgaccgc catcgcggat catgccgacc agcccgtgtc ggtgctccac
     4321 gcgctggccg aaggggagcg ccaccagacc ctcgtcgaat ggaacgattc tgcccgcgaa
     4381 gggttcgcag cgcacacgtt ccagcgtctg ttcgagatcc aggtgaagtc cacgccgcgg
     4441 gcgcgggcgg ccctgtgcga aggggaggcg gtgacctatc gcgagctcaa tgcccgggca
     4501 aaccactgcg ccgcgctgct ggccgggcag ggggccgggc cggagacgcg ggtcgctctg
     4561 gtggccgacc gcgggatcga ttttctggtg gcgatcctgg gtgtgttcaa ggccggcgca
     4621 gcgtacctgc cgatcgaacc cagggacccg acgccccggc aggcacagat tctggcgcgc
     4681 agtggcgcct cgctggtcct ggtgtcgcgg gagctcgctc ccgccctggc cgaggtcgcc
     4741 gagtcgttgc ctgctggcgc caggccacgg ctgttggtga tggaggaggc ctgccgcggc
     4801 gagggagggc agacgaaccc ggagccgcgc cagcagggga acggcctcgc ctatgtgatc
     4861 tacacctcgg gctccaccgg catgcccaag ggggccatga tcgagcagcg cggcatgctc
     4921 aaccacctcc tggccaaggt gaccgacctg gacctcacgg cgacggaccg ggtggcgcag
     4981 acggcatcgc agtgttttga catctcggtc tggcagttcc tggtgcctct gatcgtgggc
     5041 ggctccgttc acatcgtccc ccgcgaagtg gtccaggatc cggtacgcct gctgcctgcc
     5101 ctggaggagg cggggatcac ggtgttcgag acggttccct cgctgctgcg cctcctcgtc
     5161 gcggaggccg ccggacgggg acccgcgagg ccgccgctgc ccgccctgcg ctggcttctc
     5221 tcgaccggcg aagcgctgcc gccggacctt tgcgacgagt ggcaccggca ctgccccggc
     5281 gtgcccctgg tcaacgccta cggtccgacc gagtgctccg acgatgtcac ccatcaagtg
     5341 ctgcgggccg ctccgggacg ccgttccgac cggatccccg tggggcgtcc catcgtcaat
     5401 acccagctct atgtggtggg ccgctccctg gagcctttgc ccatgggagt ggcgggcgag
     5461 ctctgcgtgg gagggttcgg agtcggacgc ggttatctgg gcgagcctgc gcgcaccgcg
     5521 gaggtcttcg tacccgatcc gttcgcggag gcgccgggca gccgcctcta tcggaccggc
     5581 gaccttgccg cctgccagcc ggacggcgcc actgatttcc gcggccgccg ggatcatcag
     5641 atcaaggtga ggggtttccg tatcgagctg ggcgatatcg aaacggctct ggaacgccac
     5701 cccgccgtcg ccgaaacagt ggtcgtcaac cgccggctcg gggaagggga cacgcgtctg
     5761 gtggcctacg tcgtcgccgc tcaggaccgg cggcccgacg ccgcagagct gcgggcgttc
     5821 accaaggggg agctccccga ctacatggtc ccgtcggcct tcgtcttcct ggatgctctg
     5881 ccgctgacgc ccaacggcaa gatcgaccgt aaggccttgc cggcgccgga gccggcagag
     5941 ggcgagaccg ggctcaccgc gactcgcaat cctgtcgagg atctggtcgc gggcatttgg
     6001 gcacaggggc tcgggacgga gcaggtgaac gcgcacgatg acttcttcag cctgggtggc
     6061 cactcgctcc tggccacgca gatcctggcg cgggtccgtc aggcgttcca agtcgatctc
     6121 tcgctgcggg cccttttcga ggctccgact ctttcgggtt tcgcggcgcg ggtcgaggag
     6181 gccatgaagc aggcgcgggg agtcgcggct ccgcctctgc ggcgggtccc cggaggcgcc
     6241 ggcggcgagc ggatgcctct ctccttcagc cagcagcggc tctggttcct cgaccagctc
     6301 cagccgggca gtcccgcgta caacctgccg agcgccctac gtctgcaggg cccgctggac
     6361 attgcggcgc tgggcgccgc actggacgag atcctccgcc gccacgagtc gctacgcact
     6421 cgctttgaag cggccggcgg cgagcctttc caggtgatcc aggaagcgga gcccctgcct
     6481 ctaccgctgc tcgacctctc cgccctgccg caggtatcgc gggagcacga ggtccgccgc
     6541 cttgcgtccg aggaggcgaa gcgtcctttc gatctcgccc gcggaccgtt cctgcgggtc
     6601 accctggcgc ggctcgcgcc ggaggagcac accgtcctct tcaccaccca tcacgtggcc
     6661 agtgacgcct ggtcgatggg agtgctgatc cgcgaggtcg gcgccttgtt ctcggcgttc
     6721 gccgagggta ggccgtcgcc tctctcgcag ctggccctcc agtactcgga cttcgcctat
     6781 tggcagcgga actggctgcg cgacgagaca ctggaggcgc agctcgctta ctggcgtcag
     6841 cggctggcga aggtgcggac cccgataaac ctgacggtgg acaaggcgcg gccggcggtc
     6901 cagacgttcc ggggcgcctc cctggtcttc tccctctcgg ccgagctcgg ccgcaaggcc
     6961 aaggcgctcg cgcggcagca gggggtgacg ctgttcatgg ttctgctggc cgcctaccag
     7021 accctgctca gccactggtc cgacagcaag gacatcctcg tcggctcgcc catcgcctac
     7081 cgcaaccacg tcgagctcga ggagctgatc ggcttcttct ccaacgtcct ggtgctgcgg
     7141 accgagctgt ccggcgatcc gaccttcaac gagctgctgg cgcgggttcg cgaagtaacc
     7201 ctgggcgctt actcccacca ggacgtgccg ctcgagaagc tggtggagga gttgcggatc
     7261 gagcgcaatc tggcctacaa cccgctggtg caggtcgcgt tcacgctgga caacacaccg
     7321 caggagaacc tgggcatctc agatcttgcc ctcagccctc tggacgtggc ggtggagacc
     7381 accccctggg acctgacctt gaacgtggtg gacggcgggc gcgatctcgc cgcgacgctg
     7441 cagtac
//
LOCUS       DASPJI010000812         5214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_399600, whole genome shotgun sequence.
ACCESSION   DASPJI010000812 DASPJI010000000
VERSION     DASPJI010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399600"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1858)
                     /locus_tag="VEL74_22890"
     CDS             complement(<1..1858)
                     /locus_tag="VEL74_22890"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYG65447.1"
                     /translation="MRKWSLFAVMLLLILPVAAFAAPKWLSVDEQIQKDKVQKGSKLE
                     KLIRENQDFQLLREEESNDKIPVPAWLRVIYRKAHPEVEYSPADPMGGYPHVLKEVHE
                     WMITHQDLVPGNPSHSKRGDRVLTMVEEVTAGTNQRVSGAAANPRSESDVRVNYWDNS
                     KIIGGANNIGGNGQQLQFYSTDGGATWGQTYLPLPTGAAFNSDPTVDWTSDGTAWATT
                     MGINSSGTQLRLHSFKSTNSGATWTLEGTISGSQTNTDKQMMWVDHSATSPFKDTIYV
                     CWHNGLPQFVNRRTPGTSGTWGTPIQISGAETTGTAIGCDVKTNSFGDAFVFWPATSN
                     RRILVSKSTNGGTSWGAPVIVTTAFDGYDIGVPAFNGRRILIYATGGAYRTATKNNVY
                     VAWTDLSGDSGCTSAANEPGSNTGSTCKTRIFVSRSTNGGSTWSTPVKINNQAGLNDQ
                     FNPWLVVDETNGQLAIMYYDTVDDPGRKKTNVYYQASFDDGASWSTPLKVTTAQTDET
                     VAGSDSGNQYGDYNGMSGYAGVFFPVWTDRRNNAREEIWTAAIEENGTACTPPSAPAG
                     VSASLSGSQINLSWGAVAGATEYRILRSTTPGGPYTQIATTTSTSYANTGLAC"
     gene            2393..3139
                     /locus_tag="VEL74_22895"
     CDS             2393..3139
                     /locus_tag="VEL74_22895"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYG65448.1"
                     /translation="MLKDFLRTVSLFRDLAEEQLAHLASIAGEVSHPAKQVIYREGDP
                     VDAFYVVKDGLVTVFREVPGQPLEILARLERGGYFGEMGLLNDKARRYASARTLLPSV
                     LLRIEKSDLIALLTANPALELKFRAEVIRRHGVSVSALLALAGQRDVRIQLGAEATAD
                     MEDGSRHALLLDNLSLGGVGLSRVPAEWYVGQPVCFTLGTQAEPALLRVRGKITWREG
                     EHAGIAFDAETAGDARQVYRALRSFLDTHK"
     gene            3148..3600
                     /locus_tag="VEL74_22900"
     CDS             3148..3600
                     /locus_tag="VEL74_22900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65449.1"
                     /translation="MSGPRPEDAQSIDAVVTALYEVLSGPAGPRDWERQRFLLAPWAL
                     LMPTRPRPDGGHGVEVFDAGGYAASRGPLLLQTAFYETEIARRVERFGNIAHVWSTYE
                     SRRAPGEEPFMRGINSIQLYHDGGRWWVVAVLWDNEREGHPLPAAYLP"
     gene            3610..4779
                     /locus_tag="VEL74_22905"
     CDS             3610..4779
                     /locus_tag="VEL74_22905"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HYG65450.1"
                     /translation="MRRCPVSGLAAAALLLSLSGCSTTTRLRCGAPATLAPVAPVSAA
                     ECGVEQLPAMGVWQFENLVLEGGGVKGIAYVGAVQVLHEEGVLGNLKRVAGTSAGSIM
                     AAFIALRYPIEQLEQLMMTTDFTKFEDDGDRGPVRLAEDFGWYSGQYFRDWVGCQVEK
                     VTGNAGFTFQDLHAATNPDGSRKYLDLYIVTTDLSIKDWKVLSHETVPCMPIAEAVRM
                     SGSLPLVWNALREDLSVYAGPDCSQKRPAGATSVFVDGGVLRNYPLDLFDRAKFIHEH
                     APDPEKEEVNGLTLGLHLNTPAGTGADRPIDSLPDYVKSLFETILGLQVDQFEADPCS
                     LARSAQIDDLGIPTADFNLTEPQKRALICSGRRHTGEYLQRWRPDEVMKRCQSVR"
     gene            4905..>5214
                     /locus_tag="VEL74_22910"
     CDS             4905..>5214
                     /locus_tag="VEL74_22910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG65451.1"
                     /translation="MPIPRSSIRQEMLADLAGPGGVWVLTGPPGIGKSHLARRFQDEL
                     PSGARMVWARGISRQEALRPFWSVAHELQEGARARKIAAVARGVAREVRYLKDSVGAL
                     V"
BASE COUNT          782 a         1679 c         1882 g          871 t
ORIGIN      
        1 cgcaggccag gccggtgttg gcgtagctgg tgctggtggt ggtggcgatc tgggtgtagg
       61 gaccgcccgg cgtggtcgag cgcaggatgc ggtactcggt ggcgccggcg acggcacccc
      121 acgacaggtt gatctgcgag ccggagaggg aggcggagac gccggccggg gcggacggcg
      181 gcgtgcaggc cgtgccgttc tcctcgatcg ccgcggtcca gatctcctcg cgggcgttgt
      241 tgcggcggtc agtccacacc gggaagaaga cgccggcgta gccggacatg ccgttgtagt
      301 cgccgtactg gttgccgctg tcggagccgg cgaccgtctc gtcggtctgg gcggtggtca
      361 ccttgagcgg cgtgctccag ctcgcgccgt cgtcgaagga ggcctgatag tagacgttgg
      421 tcttcttgcg gcccggatcg tccaccgtgt cgtagtacat gatcgcgagc tggccgttgg
      481 tctcgtcgac caccagccag gggttgaact ggtcgttcag cccggcctgg ttgttgatct
      541 tgaccggggt cgaccaggtg gagccgccgt tggtggagcg ggagacgaag atgcgggtct
      601 tgcaggtgga gccggtgttc gagccgggct cgttggcggc gctggtgcag ccggagtcgc
      661 ccgacagatc ggtccaggcc acgtagacgt tgttcttggt ggcggtccgg taggcgccgc
      721 ccgtggcata gatcaggatg cggcggccgt tgaacgcggg cacgccgatg tcatagccgt
      781 cgaaggcggt ggtgacgatg accggggcgc cccagctcgt gccgccgttg gtcgacttcg
      841 agaccaggat gcggcggttg ctggtggccg gccagaagac gaaggcgtca ccaaaggagt
      901 tggtcttgac gtcgcagccg atcgccgtgc cggtggtctc ggcgccgctg atctggatcg
      961 gcgtgcccca ggtgccggag gtgccggggg tgcggcggtt gacgaactgc ggcaggccgt
     1021 tgtgccagca cacgtagatg gtgtccttga acggtgaggt ggcgctgtgg tccacccaca
     1081 tcatctgctt gtcggtgttg gtctggctgc cggagatggt gccctcgagg gtccaggtgg
     1141 cgccgctgtt ggtggacttg aaggagtgga ggcggagctg ggtgcccgag gagttgatgc
     1201 ccatcgtggt cgcccacgcc gtgccgtcgg acgtccagtc caccgtcggg tcggagttga
     1261 aggccgcgcc ggtcgggagc ggcaggtagg tctggcccca ggtggcgccg ccatcggtgg
     1321 agtagaactg gagctgctgg ccgttgccgc cgatgttgtt ggcgccgccg atgatcttgc
     1381 tgttgtccca gtagttcacc cggacgtcgg actcggagcg cgggttggcg gccgcgccgg
     1441 agacccgctg gttggtgccg gcggtgacct cttcgaccat ggtgagcacc cggtcaccgc
     1501 gcttcgaatg cgaggggttg cccggcacga ggtcctggtg ggtgatcatc cactcgtgga
     1561 cctccttgag gacgtgggga tagccgccca tcggatcggc ggggctgtac tcgacctcgg
     1621 ggtgggcctt gcggtagatc acccgcagcc aggccggcac cgggatcttg tcgttggact
     1681 cctcctcgcg gaggagctgg aagtcctggt tctcacggat cagcttctcg agcttgctgc
     1741 ccttctgaac cttgtccttc tggatctgct cgtcgaccga cagccacttg ggggccgcga
     1801 aggcggccac cggcaggatc aacaggagca tgacggcaaa caaagaccat ttacgcattt
     1861 ctaacaatcc tcctgaatag gcactacgtt caagcaccac gtcacggaca cattgggacg
     1921 cctcttcctg cctcaaacga ctcgaggagg tggtggctcc accctgatat ccgagacgaa
     1981 accggccaac gtcagaaatg tagaaaagcc cgaacgacct ctgcacggtc ggtgcgccaa
     2041 cctttgatca cggctatact caaaggcgcg taccgacgcc gatgacaaca tcccaacccc
     2101 ttgctgctgc gtatcgcggc cgtgacgggg cagggtgaga gtcgaggtgt gcggtcactt
     2161 acctagagaa gtgcctgcca gggtcaaaag cgcgccaacc tttttccgga aagtttcggc
     2221 gccgtcaccg ggccgccctc acccgcgggc aggaggggta gggagaccgg agagccgggc
     2281 ttgcctgttg ggcctcttgg acacacaata cagcggctcg tgacaagctc tgaggtcctg
     2341 ccgtcgatcc gccgtgggtc ggtccacggg aggcgcgagg atggaggccc ttttgctgaa
     2401 agacttcttg cgtacggtga gcctcttccg ggacctggcc gaggagcaac tcgcccacct
     2461 ggcgtccatc gccggcgagg tgagccatcc ggccaagcaa gtcatctacc gggagggtga
     2521 ccccgtcgat gccttctatg tagtgaagga cgggctggtg accgtcttcc gggaggtgcc
     2581 cggccagccg ctggagatcc tggcgcggct ggagcgcggc ggctacttcg gcgagatggg
     2641 gctgctgaac gacaaggcgc gccgctacgc ctcggcccgc acgctcctgc cctcggtgct
     2701 gctgcgaatc gagaagagcg acctgatcgc gctgctgacg gcgaacccgg cgctggagct
     2761 caagttccgc gccgaggtca tccgccggca cggggtgagc gtctcggcgc tcctggcgct
     2821 ggccgggcag cgggacgtcc gcatccagct cggggcggag gcgacggcgg acatggagga
     2881 cggctcccgg cacgccctgc tgctggacaa cctgtcgctg ggcggggtgg ggctgtcgcg
     2941 ggtgccggcg gagtggtacg tggggcagcc ggtctgcttc accctcggga cccaggccga
     3001 gccggcgctg ctgcgcgtcc gcggcaagat cacctggcgg gagggggagc acgccggcat
     3061 cgccttcgac gccgagaccg ccggcgacgc ccgccaggtc taccgcgcgc tccggagctt
     3121 cctcgacacc cacaagtgac agggatcatg agcggacccc gacccgagga cgcgcagtcc
     3181 atcgacgccg tggtgacggc gctgtacgag gtcctctccg gtcccgccgg gccgcgggac
     3241 tgggagcggc agcgcttctt gctcgccccc tgggcgctgc tgatgcccac ccggccccgt
     3301 cctgacggcg gccatggggt ggaggtcttc gacgccggag ggtacgccgc ctcgcgcggg
     3361 ccgctgctcc tccagaccgc cttctacgaa acggagatcg cccgccgcgt cgagcgcttc
     3421 ggcaacatcg cccacgtctg gagcacctac gagtcgcgcc gggcgccggg ggaggagccg
     3481 ttcatgcgcg gcatcaacag catccagctc taccacgatg gcggccgctg gtgggtcgtc
     3541 gccgtcctct gggacaacga gcgggagggg cacccgctgc cggccgccta cctgccctga
     3601 ggagatccca tgcgcagatg ccctgtttcc ggcctcgcgg ccgccgccct cctcctctct
     3661 ctgagcggct gcagcaccac gacccggctc cgttgcggcg ctccggccac cctggctccg
     3721 gtggcgccgg tgtcggccgc ggagtgcggg gtggagcagc tgccggcgat gggggtctgg
     3781 cagttcgaga acctcgtcct cgagggggga ggcgtgaagg ggatcgccta tgtcggcgcg
     3841 gtccaggtgc tccacgagga gggggtgctg gggaacctca agcgggtggc cggcacctcg
     3901 gccggctcga tcatggccgc cttcatcgcg ctgcgctacc ccatcgaaca gctcgagcag
     3961 ctgatgatga ccaccgactt caccaaattc gaggacgacg gcgaccgcgg gccggtccgc
     4021 ctggccgagg acttcggctg gtacagcggc cagtacttcc gggactgggt gggctgtcag
     4081 gtcgagaagg tcaccggcaa cgccggcttc accttccagg acctgcacgc ggcgaccaac
     4141 ccggacggct cccgcaagta cctggacctc tacatcgtca ccaccgacct gtcgatcaag
     4201 gactggaagg tcctctccca cgagaccgtg ccctgcatgc cgatcgccga ggcggtgcgc
     4261 atgtcgggat cgctgccgct ggtctggaac gcgttgcgcg aggacctgag cgtctacgcc
     4321 ggcccggact gctcgcagaa gcggccggca ggcgcgacga gcgtcttcgt cgacggcgga
     4381 gtgctgcgca actatccgct ggacctcttc gaccgggcga agttcatcca cgagcacgcc
     4441 cccgacccgg agaaggagga ggtcaacggc ctcaccctgg ggctccacct caacaccccg
     4501 gccggcaccg gcgccgaccg tcccatcgac agcctccccg actatgtgaa gagcctgttc
     4561 gagaccatcc tggggctgca ggtggaccag ttcgaggccg atccctgcag cctggcccgc
     4621 tcggcccaga tcgacgacct gggtatcccc accgccgact tcaacctgac cgagccgcag
     4681 aagcgagccc tgatctgctc gggccggcgc cacaccggcg agtacctgca gcgctggcgg
     4741 ccggacgagg tgatgaagcg ctgccagagc gtgcggtgag gcggggaggg acgagagccg
     4801 agtctggcgt ggagtggaaa aagtgcgtcg tgaagatgtc ggccctaccc ggttttgccc
     4861 acgggatcac gtagcggggg aatttttggt agtcttccct tccggtgccg atcccccgtt
     4921 cctcgatccg tcaagagatg ctcgccgacc tcgccggccc gggaggcgtg tgggtgctga
     4981 ccggtccgcc gggcatcggc aagtcccatc tggcgcgccg gttccaggac gagctgccgt
     5041 ccggcgcgcg gatggtctgg gcgcggggga tctcccggca ggaggcgctc cgccccttct
     5101 ggagcgtggc gcacgagctc caggaggggg cccgggcgag gaagatcgcg gccgtggccc
     5161 ggggtgtcgc ccgggaggtc cgctacctca aggactcggt gggagccctg gtcg
//
LOCUS       DASPJI010000813        10164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_853990, whole genome shotgun sequence.
ACCESSION   DASPJI010000813 DASPJI010000000
VERSION     DASPJI010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10164)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10164
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853990"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VEL74_22915"
     CDS             complement(<1..830)
                     /locus_tag="VEL74_22915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HYG65452.1"
                     /translation="MTPPADGREIVWHAGEERLEVPDRPIIPYIEGDGTGPDIWRATR
                     RVLDGAVEKVYRGAKSIAWFEVLAGEKAFNQTGEWLPEDTLDAIRQYRVAIKGPLTTP
                     VGGGIRSLNVTLRQVLDLYACVRPVRWFEGVPSPVKEPGKVDMVIYRENTEDVYAGIE
                     WPANSDDAKKVIDFLASMGKQVRPDSGIGIKPVSATGSKRLVKKALAYAVECRRESVT
                     LVHKGNIMKFTEGAFRDWGYEAAKESFAGQTVTENEVWEQHNGKAPAGKIVVKDRIAD
                     S"
     gene            complement(1759..2118)
                     /locus_tag="VEL74_22920"
     CDS             complement(1759..2118)
                     /locus_tag="VEL74_22920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65453.1"
                     /translation="MPSSDDRTPSRVDLSRPTESTRRVLHELVVHVYFHPTPEEVEKA
                     REDPGNHWVPDYGPDAARLHVFHAYGRWFAVWEALEEKKEADANPSLPEWRRWSVVRI
                     VEDRDPSSETGVTFLEV"
     gene            complement(2473..2709)
                     /locus_tag="VEL74_22925"
     CDS             complement(2473..2709)
                     /locus_tag="VEL74_22925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65454.1"
                     /translation="MASLNITVPDSLYRQLEDLAKGEKVSLDQLVSTVLTQRLSALNE
                     GDYLERLAREGNREAFERVLAKVPDVEPDPWDRW"
     gene            complement(2712..3131)
                     /locus_tag="VEL74_22930"
     CDS             complement(2712..3131)
                     /locus_tag="VEL74_22930"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HYG65455.1"
                     /translation="MPLARSVLDTSILVSAFRSRRGPAARLIAALGTGLFEVCVSVPL
                     VLEYEEVLCRATGLAREEVLDVIRYMCSIAHRQKIFFLWRPLLRDPDDEMVLELALAS
                     RAKYIVTYNSRDFIEAERFGVEVVTPKEFLGILGVEG"
     gene            complement(3165..3974)
                     /locus_tag="VEL74_22935"
     CDS             complement(3165..3974)
                     /locus_tag="VEL74_22935"
                     /inference="COORDINATES: protein motif:HMM:NF015586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65456.1"
                     /translation="MTGSDERGRVGIDAGASLWKLARAGHPEKEILPAGAVQEVRRRI
                     AAWNPGEVRVTGGGGARLAAALEGIPVRQVSEFDAWARGAAHLAGSAGWALPPRYMLV
                     SLGTGTSMLEIGDSVHRVGGMALGGGTLLGLARLLCGTGSFAEVTALAARGDRRGVDL
                     LVRDVYPEGGIALPPDLTASNFAKLCSREPADLAHALMGLLGENIAIACAAVAHAQDL
                     DTLVFGGSPLEGNPTLEEILRLTVQMMGRESRFLPDGAFCGAVGAALVEPL"
     gene            4425..4811
                     /locus_tag="VEL74_22940"
     CDS             4425..4811
                     /locus_tag="VEL74_22940"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HYG65457.1"
                     /translation="MDWEPLIAAAAAVRSRAYAPYSHFPVGAAILMEDGSIHAAANVE
                     NCIPALSICAERNAMSAAASAGLRKPVAVAVVTDTSPPSRPCGLCRQTLTEFAGDLPI
                     LCCNEQGEREEVRLSEIFPQAFSLAP"
     gene            complement(4889..5203)
                     /locus_tag="VEL74_22945"
     CDS             complement(4889..5203)
                     /locus_tag="VEL74_22945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65458.1"
                     /translation="MNRLAALVLLLAAAVPAAPAFAFDFDVTPTRILCQWVTQRGAGE
                     TEEAAYDNALYQLKLDYFVLRSTRVSADCPEDEDLNTPGLEVPLLCRAEIRACVSPKP
                     RF"
     gene            complement(5263..5676)
                     /locus_tag="VEL74_22950"
     CDS             complement(5263..5676)
                     /locus_tag="VEL74_22950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65459.1"
                     /translation="MLKRTLAALFLSLLATTGAATAQSWSDLQVTSVLHLDKLPPWIE
                     DSRRFAEYQHDMVKMAERWLVEQGHHLEADGPIVKLPIPGHGFVAVFDTRGPDVTVVF
                     GQSIERPDRERVDVTMTVLFERSLRVDVPNIYNGI"
     gene            complement(5736..6161)
                     /locus_tag="VEL74_22955"
     CDS             complement(5736..6161)
                     /locus_tag="VEL74_22955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65460.1"
                     /translation="MLKRTLAVLLFSVLASAAAATAQPPSWSDLQASSVLHLAKLPPG
                     LSDAQRFAQYQHAAIQVGKQWLAEHGYLFDANPAISAQVTNQGVVVTYNAGGHSITVI
                     YGPGPIIERPDRDRVDIRMTVLLPRALEVVDVLQINNGY"
     gene            6314..8962
                     /locus_tag="VEL74_22960"
     CDS             6314..8962
                     /locus_tag="VEL74_22960"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG65461.1"
                     /translation="MKYRFGEFVLDLEAGRLLGPDGEVRLRPQPFRLLQVLVENAPRI
                     LSQEELLDLVWGVEHLSPASVKQAVSEVRQALRDDPSRPAAIETVPRRGYRFIAALAR
                     VEEEAPQLPVVSADAEFGTWPVVLSERARRVVEEAIEEDAAAPPAAAVPASGSRRAAL
                     ALVIGLAALGITVFALLVRATPDGAPPAGKAAEAPAARAAARPATRPAARPAVAVLGF
                     KNLSGDPGDDWISGALAEIIGFELAAPGRVRLIPGENVERMRRELALPHAERHAPATL
                     ARIGRNLGTDLVVAGSYLRTPGEDGEDKLRVQMVVQSVATGDTVAWARHTGAPESLIE
                     LATAAVRGVQVGLGTAVPGIAAGGDAAAALASNAASLQLYSEALARLRVWNAPAALPL
                     LEQAAAADPGNPFVHDALAGALAELGFEGEGKKAAGRAVGLARELPQELRLGIEGRFH
                     ALSGAWEEAAGIYTALWRLHPDDLEPGLRLAHAQRQAGQTGGALATVAALRRLPSPAG
                     DDPRIDLEEADAAWQGADFARARDAASRAVAKSERRGTALLTAAGLFNRAWSALRLGD
                     SRAALADFRQAHDLYIRMGDRGAAMGALLGSASVLQATGRPAEARQAYERAIPVLRES
                     GDRRREAKALNNFAALLADQGDFDAVTRLLERSLEVKREIGDLQGAATTLANLGNLLR
                     QRGDLAGARRRTDEALALSRRLGDAHGTALALRGMARVLDHQGQGAEARAALEEALEL
                     GRGSGDAEGVAQTRIALGELARRAKRPDEAREHFREALAEFERLGRTGNVAHSRIALA
                     EIDLEQGRLDAARAGFAAALALARSLENDLYEAHAHSGLAEVATRKGDVTGARGEHER
                     ALALWLKLEDEEKAARARKALQELKG"
     gene            9014..9682
                     /locus_tag="VEL74_22965"
     CDS             9014..9682
                     /locus_tag="VEL74_22965"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HYG65462.1"
                     /translation="MKAFLAALLLALPLAAAAQPPAPEAKPAPAPAPAPSPAEEEVGV
                     LETTLGTMVIRFHEQDAPQTVANFKKLVRDGFYDGRNFYRIVKGHVIQAGDGGENDKP
                     KIKGEFNSRPFVPGAVGLARGGDPDSGSTEFFICHVARPHLDGKYANFGQLVEGMDVM
                     EKIASVEVEEQWIGDEEPKVAFHGPKTPVVISKARIEKRALPPAAPAAEPAPAPAPVP
                     PPAR"
     gene            complement(9683..>10164)
                     /locus_tag="VEL74_22970"
     CDS             complement(9683..>10164)
                     /locus_tag="VEL74_22970"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG65463.1"
                     /translation="VALCRRHPGLRGVVLDLPAAIEQSAPLLAKEAMGERVVHRAGDA
                     MADKLGAGAWDVVLLFQLAHHFGEAANRGLVERIARSLRPGGVLVILEVIRRERPGEG
                     GQIGGLFDLYFAFLSRAGTWSFAEMADWQRSAGLVPRRPVRLRTAPGLGLQIGTKPR"
BASE COUNT         1547 a         3605 c         3563 g         1449 t
ORIGIN      
        1 gagtcggcga tgcggtcctt gaccacgatc ttgcccgcgg gggccttgcc gttgtgctgc
       61 tcccagacct cgttctcggt gacggtctgg ccggcgaagc tctccttggc cgcctcgtag
      121 ccccagtcgc ggaaggcccc ctcggtgaac ttcatgatgt tgcccttgtg caccagggtg
      181 acgctctcgc gccggcactc gacggcgtag gcgagggcct tcttgaccag ccgcttgctg
      241 ccggtggcgg agaccggctt gatgccgatg ccgctgtccg ggcggacctg cttgcccatg
      301 gaggcgagga agtcgatgac cttcttcgcg tcgtccgagt tggccggcca ctcgatgccg
      361 gcatagacgt cctcggtgtt ctcgcggtag atgaccatgt cgaccttgcc cggctccttg
      421 accggcgagg ggaccccctc gaaccagcgc accgggcgca cgcaggcata gaggtcgagc
      481 acctggcgca gcgtgacgtt caagctgcgg atgccgccgc cgaccggggt ggtcagcggc
      541 cccttgatgg cgacgcggta ctggcggatg gcgtcgaggg tgtcctcggg cagccattcg
      601 ccggtctggt tgaacgcctt ctcgccggcg aggacctcga accaggcgat ggacttggcg
      661 ccgcggtaga ccttttcgac cgcgccgtcg aggacgcggc gggtcgcgcg ccagatgtcg
      721 ggcccggtgc cgtccccctc gatatagggg atgatcggcc ggtcgggcac ctcgagccgc
      781 tcttcacccg cgtgccagac gatctcccgg ccgtccgcgg gcggtgtcaa gttgttgaat
      841 gtcctggaca aacccgtgct cctctcggat ggaaacgtag ggggaaacga gacgacgagc
      901 ccggccctcg ggccgggagc cccgcgtacg gtatcacaag ggtcaagggg agcgtgcccg
      961 attggatagg ccccccactg gccgccggac aagctccccc ttccttatta gataggggag
     1021 cgggccggtc cggggatggc cggcaacccg gtccaaactt tcttgaaagt cgttgtcctc
     1081 gaaacacccc ccagcggcaa catacatggt gaaggatcat ccatcctgcc cggcggactt
     1141 gaaccggatc ccgccgggcc tttttttgcc cgctccccgc ctgccagccc gcctcccggg
     1201 gtcctctcca agaaagtcga aaaaagttcg attcccttgt cccgctttgg gcggctcaag
     1261 cgtccatatc tcttgaagga tcatccatcc tgcccggcgg atcgaaccgg atctcccgcc
     1321 gggccttttt tattctcccc tcttccagaa aagcagagac gctcccgggc tggacgaggg
     1381 ggacggctcc cattggcgcc aactcgagcc gcagcggggt ggtggtgcct cccccctggg
     1441 tggccgggcg ggagggagcg gggaaggtca ggcggggggt tcttcttcgc gccagccatc
     1501 cgaagcgtcc gggaagctcc ccgctccctc ggaatccctc tttttgaatt ggaacgcatc
     1561 cgggacgctc gggaagcatc cgagggcgcc ggacactttc caggcgagtt ggaaccgatc
     1621 cggaacgctc gggaagcatc cggagcgtca ggatgccatc cgcggcgtct ggatagcatc
     1681 cgcggcgacg gggcccttcc tttttcttgg ggaatgaagg gcgggccccg ggacgcggga
     1741 cccgccgtca gggcggcgtc agacctcgag aaaggtgacg cccgtctcac tggacgggtc
     1801 ccggtcctcc acgatccgca ccaccgacca gcgccgccac tcggggagcg acggattggc
     1861 gtccgcctcc ttcttctcct ccagcgcctc ccagaccgcg aaccaacggc cgtaggcatg
     1921 gaagacgtgc aggcgcgccg cgtcgggacc gtagtccggc acccaatggt tgccggggtc
     1981 ctcccgggcc ttctccactt cttcaggcgt gggatggaag tagacgtgga ccaccagctc
     2041 gtgcaaaacg cggcgggtgg attcggtggg acgggacaga tcgacgcgcg aaggcgtacg
     2101 atcatctgag ctgggcatga ggtgatactc ctgtccggct aggcctcggg gagtgagctt
     2161 ccaactcctc cccttggccg tctgggaccc tgggatcccg gatgagctac gcgctcacct
     2221 cggagacaca gcggccctta cggctgtgct gtgaagctgc ggcttgatgt tcctcccggc
     2281 gacctccttt ctggtacggc ttacgaccgc ccaagactac aacgcgaccg gaggtgcgtc
     2341 aagaggaaaa tgggggattc ctgcgggaaa gttgtggagt gcggagcggt gtgaaaaacc
     2401 cccctgtgac ctcctcgctc cctaccgccc gaccgcccag gggggaggca ccaccgccct
     2461 cctagctcag ccctaccagc gatcccaagg atccggctcg acatcgggga ccttcgccag
     2521 gacgcgctcg aaggcctcac ggttgccctc acgggcaagc cgctccaggt agtcaccctc
     2581 attgagggct gaaagccgtt gagtgaggac cgtgctgacc agttgatcca aggacacctt
     2641 ctcccccttc gccagatcct cgagttggcg gtagagagaa tcaggcactg tgatgttgag
     2701 gctcgccatg actagccctc cactcctagg atgccaagga attccttcgg tgtcaccacc
     2761 tcgaccccaa aacgctcagc ctcgatgaag tcccgcgagt tgtaggtcac aatgtacttc
     2821 gctcgggagg caagggccag ctcgagcacc atctcgtcgt ccgggtcgcg caggagcgga
     2881 cgccagagaa agaagatctt ctggcgatgc gcgatcgagc acatatagcg aatcacgtcc
     2941 agaacctcct cgcgtgccaa ccccgttgca cgacagagga cctcttcata ctccagaacc
     3001 agaggcaccg agacgcagac ctcaaagaga cctgtaccca acgctgcgat taggcgtgca
     3061 gcgggccccc tacgagagcg gaaagcagag acaaggatac tcgtgtcaag gactgagcgc
     3121 gccagcggca tggctccatg atatctcctc tgatcgaaga gtccctacaa gggctccacc
     3181 aacgccgccc ccaccgcccc gcagaacgcc ccgtccggca ggaagcgcga ttcccggccc
     3241 atcatctgga cggtcaggcg gaggatctcc tccagggtgg ggttgccttc cagggggctg
     3301 ccgccgaaga cgagggtgtc gagatcctgg gcatgggcca cggcggcgca ggcgatggcg
     3361 atgttctcgc cgaggaggcc catgagggcg tgggcgaggt cggcgggctc ccgggagcat
     3421 agcttggcga agttggaggc ggtcaggtcc ggaggcaggg cgatacctcc ctcggggtag
     3481 acgtcgcgga cgaggaggtc cactccccgg cggtcgccgc gcgcggcgag agcggtgacc
     3541 tcggcaaagg agccggtccc gcagaggagg cgcgccaggc ccaggagggt gccgcctccc
     3601 agcgccatgc ctccgacccg gtggacggaa tcgccgatct ccagcatcga cgtccccgtt
     3661 cccaggctca ccagcatgta gcgcggcggc agggcccagc cggcgctccc ggccagatgc
     3721 gcggcgccgc gggcccaggc gtcgaactcg gatacctgcc ggacggggat cccttcgagg
     3781 gcggccgcca gccgcgcgcc gccgccgccg gtcacccgga cctccccggg attccaggcg
     3841 gcgatccggc gccggacttc ctggaccgcg ccggcaggga ggatctcctt ctccggatgg
     3901 ccggcccgcg cgagcttcca gaggctcgct ccggcgtcga tccccactct gccccgctcg
     3961 tcgctcccgg tcacgcgctg gtgcctccgc tgaatcgtcc ctatctaata aggaaggagc
     4021 tgcttctcca acggcgcgtc gcagcctccg gcggcgtgcc ggaagcttcg gcggcttggc
     4081 gaagagtacg acagcgcgcc ggagccttcg gcagcccgtc gaagcttcca aggagccgtc
     4141 gcaaggttgc gacgcgccgc cagagattgc aacggctcgc cgaagagtac gacgccggtc
     4201 cggagtctcc ggcagcccgt cgaagcttcc aacggcccgt cgaaggatac gacggcgcgc
     4261 cgtatccttc ggcggcgcgt tggcagcttc ggcggcgcgc cggcagcttc caacgcgccg
     4321 ccgaaggttg cggcgccctg ccggagcttg caacgggccg ccggaaagtc cgacggcacg
     4381 ccggaggctt cgccggcgcg tccccatgct agattgcgca tgtcatggat tgggaaccgc
     4441 tgatcgccgc cgccgccgcc gtccggagcc gcgcctatgc cccctacagc cacttccccg
     4501 tgggcgccgc gatcctgatg gaggacggct ccatccacgc cgcggccaac gtcgagaact
     4561 gcatcccggc gctctccatc tgcgccgagc gcaacgccat gtccgcggcg gcctcggccg
     4621 ggctccgcaa gccggtggcc gtcgccgtgg tcaccgacac cagccccccg tcccgcccct
     4681 gcggcctctg ccgccagacc ctcaccgagt tcgccggcga cctccccatc ctctgctgca
     4741 acgagcaggg ggagcgcgag gaggtgcggc tgtccgagat cttcccccag gcgttctcgc
     4801 tggcgcctta gcagccgctg aagaaaaaag ccgggggccg ggcccggagc ggcgctccgg
     4861 acccggcccc cgggacgggg agctcagctc agaacctcgg cttcggcgag acgcaggccc
     4921 ggatctcggc ccggcagagc agcggcacct ccaggccggg ggtattcagg tcctcatcct
     4981 cggggcaatc cgcgctcacc ctcgtggacc tcaagacgaa gtagtccagc ttcaactggt
     5041 acagggcgtt gtcataggcg gcctcctcgg tctcccccgc ccctctctgg gtcacccact
     5101 ggcacaagat ccgggtgggg gtgacatcga agtcgaaagc gaaggccggg gccgccggga
     5161 cggcggcggc cagcagcagg acgagggcgg ctaaacgatt catgcgattc atctccattc
     5221 tcctcacacg ggatggtcag ggctcgaggg ctccaaccca ggtcagatgc cgttgtagat
     5281 gttggggacg tcgactctca gcgagcgctc gaagagcacg gtcatggtga cgtccacccg
     5341 ctcgcggtcc gggcgctcga tgctctgccc gaagacgacg gtgacgtcgg gaccgcgagt
     5401 gtcgaagacc gcgacgaagc cgtggccggg gatggggagc ttcacgatgg gcccgtccgc
     5461 ttccaggtgg tggccctgct cgaccagcca ccgctccgcc atcttcacca tgtcgtgctg
     5521 gtactcggca aaccgcctgg aatcctcgat ccaggggggc agcttgtcca ggtggagcac
     5581 ggaggtcacc tggaggtcgg accacgactg ggcggtcgcg gcgccggtgg tggcgagcag
     5641 ggaaaggaac agggcggcaa gggtgcgctt caacatcggg attctccttt gactcagttg
     5701 ttctatcctt cgggtttccc actcggctcc ggccgtcaat agccgttgtt gatctgcagg
     5761 acgtccacga cctccagcgc gcgcggcagg aggacggtca tgcggatgtc cacccggtcg
     5821 cggtccggcc gctcgatgat cgggccgggg ccgtagatga cggtgatgga gtggccgccc
     5881 gcgttgtagg tcacgacgac gccctggttg gtgacctggg ccgagatcgc ggggttggcg
     5941 tcgaagaggt agccatgctc ggccagccac tgcttgccca cctggatggc ggcgtgctgg
     6001 tactgggcga accgctgggc gtccgagagg ccggggggca gcttggccag gtggagcacg
     6061 gacgacgcct gcaggtccga ccagctcggc ggctgggcgg tcgcggcggc ggcgctggcg
     6121 agcacggaaa agagcaggac ggcaagggtg cgcttcaaca tgggactctc ctttaggggg
     6181 ttgagtgggg aggcggcggc atcatgccgg gtgccccccg agccggcgag aagcatcggc
     6241 cggccagccg gcccgagaag tcccggaacg gtcaaggaac ggtcaaagtg aggtcaagaa
     6301 agaggttgca ggcatgaagt accggtttgg agagttcgtc ctggacctgg aggcggggcg
     6361 cctcctcggg cccgacggcg aagtccggct gcgcccccag cccttccggc tgctgcaggt
     6421 gctggtggag aacgcgcccc ggatcctctc ccaggaggag ctcctcgacc tggtctgggg
     6481 ggtcgagcac ctctcccccg cctcggtcaa gcaggcggtc agcgaagtcc gccaggccct
     6541 gcgggacgac ccctcccgcc cggcggccat cgagaccgtg ccccggcgcg gctaccggtt
     6601 catcgcggcg ctcgcccggg tcgaggagga ggccccccag ctgccggtgg tctccgccga
     6661 cgccgagttc ggcacctggc cggtggtcct ctcggaaagg gcgcggcggg tcgtcgagga
     6721 ggcgatcgag gaggacgccg ccgcgccgcc ggcggccgcc gtcccggcga gcggctcccg
     6781 gcgggccgcg ctggcgctgg tgatcggcct cgccgccctc gggatcacgg tcttcgccct
     6841 gctggtccgc gccaccccgg acggtgcccc ccctgcgggg aaggccgcgg aggcaccggc
     6901 ggcccgggcg gcggcgcgcc cggcaacccg cccggctgct cgcccggcgg tggcggtcct
     6961 cgggttcaag aacctgtcgg gcgatcccgg ggacgactgg atctcgggcg ccctcgccga
     7021 gatcatcggc ttcgagctgg ccgcccccgg gcgggtgcgc ctgatccccg gcgagaacgt
     7081 ggagcggatg cggcgcgaac tcgccctccc ccacgccgag cgccacgccc ccgccaccct
     7141 cgcccgcatc ggccgcaacc tggggaccga cctggtggtc gcgggctcct atctgaggac
     7201 gcccggcgaa gatggggagg acaagctgcg ggtgcagatg gtcgtccaga gcgtcgcgac
     7261 cggcgacacc gtggcctggg cacgccatac cggggctccg gagtcgctca tcgagctcgc
     7321 cacggcggcc gtccgcgggg tgcaggtggg gctcgggacc gccgtccccg gaatcgccgc
     7381 cggcggggac gccgcggcgg ccctggcctc caacgccgcc tcgctccagc tctactcgga
     7441 ggcgctcgcc cggctgcggg tgtggaacgc gccggcagcc ctccccctgc tggagcaggc
     7501 cgccgcggcc gatcccggca acccgttcgt ccacgacgcc ctggccggcg ccctcgccga
     7561 gctgggcttc gagggcgagg gcaagaaggc ggcgggccgg gcggtcgggc tggcgcgcga
     7621 gctgccgcag gagctgcggc tggggatcga ggggcggttc cacgccctct ccggtgcctg
     7681 ggaggaggcg gccggcatct acaccgcgct ctggcggctc cacccggacg acctggagcc
     7741 gggcctgcgc ctggcccacg cccagcggca ggccgggcag accggcggcg ccctggccac
     7801 cgtcgccgcg ctgcggcgcc tcccctcccc cgccggcgac gacccgcgca tcgacctgga
     7861 ggaggcggac gccgcctggc agggggccga cttcgcccgt gcccgcgacg ccgcctcgcg
     7921 ggcggtggcc aagagcgagc gccggggcac cgcgctcctc accgcggccg ggctgttcaa
     7981 ccgcgcctgg tccgccctcc ggctggggga ctccagggcg gcgctcgccg atttccggca
     8041 ggcgcacgac ctctatataa ggatggggga ccgcggggcg gccatggggg cgctccttgg
     8101 atcggcgtcg gtgctccagg ccaccggccg gcccgccgag gcccgccagg cctacgagcg
     8161 ggcgatcccg gtgctgcggg agagcggcga ccggcggcgc gaggccaagg cgctcaacaa
     8221 cttcgccgcg ctgctcgcgg atcaggggga tttcgacgcc gtcacccggc tcctcgagcg
     8281 ttcgctggag gtcaagcgcg agatcggcga cctccagggg gcggcgacca cgctcgccaa
     8341 cctcggcaac ctgctgcgcc agcgggggga cctggcgggc gcgcgccggc ggaccgacga
     8401 ggctctggcg ctgagccggc ggctcgggga cgcccacggc accgccctcg ccctgcgcgg
     8461 catggcccgg gtgctcgacc accaggggca gggcgccgag gcgcgggccg ccctggagga
     8521 ggcgctggag ctcggccggg ggagcggcga cgccgagggg gtcgcccaga cccggatcgc
     8581 cctgggagag ctggcgcggc gggcgaagcg gccggacgag gcgcgggagc acttccggga
     8641 ggccctggcg gagttcgaac ggctgggccg caccggcaac gtcgcccaca gccgcatcgc
     8701 cctggccgag atcgacctcg agcagggccg gctcgacgcc gcccgcgccg gcttcgccgc
     8761 ggccctcgcc ctcgcccggt cgctggagaa cgacctctac gaggcccacg cccactccgg
     8821 cctcgccgag gtcgccaccc gcaaggggga cgtgaccgga gcccgcggcg agcacgagcg
     8881 ggcgctcgcc ctctggctga agctggagga cgaggagaag gcggcgcgcg cccgcaaggc
     8941 gctccaggag ctgaaggggt gaggggccgt gctatcgttc gaatcctgaa acctgcccga
     9001 tcggaggatt gagatgaagg ctttcctggc cgccctgttg ctcgccctgc ctctcgccgc
     9061 cgccgcccag cccccggcgc cggaggcgaa gcccgcgccc gctccggccc ccgcgccctc
     9121 accggccgag gaggaggtcg gcgtcctcga aaccaccctg gggacgatgg tgatccgctt
     9181 ccacgagcag gacgcccccc agaccgtggc caacttcaag aagctggtgc gcgacggctt
     9241 ctacgacggc cgcaacttct atcggatcgt caaggggcac gtcatccagg cgggcgacgg
     9301 cggcgagaac gacaagccca agatcaaggg cgagttcaac agccgcccct tcgtgcccgg
     9361 cgcagtgggc ctcgcccgcg gcggcgatcc ggacagcggc agcaccgagt tcttcatctg
     9421 ccacgtcgcc cgcccccacc tcgacggcaa gtacgccaac ttcggccagc tcgtcgaggg
     9481 catggacgtc atggagaaga tcgccagcgt cgaggtcgag gagcagtgga tcggcgacga
     9541 ggagcccaag gtggccttcc acggccccaa gacgccggtg gtcatctcca aggcccggat
     9601 cgagaagcgg gccctgcccc cggctgcacc ggcagcggaa ccggcgccgg cgccggcccc
     9661 cgtcccgccc ccggcgcggt agctagcgcg gcttcgtccc gatctgcagc ccgagaccgg
     9721 gggcggtccg cagacggacc ggcctccggg ggacgaggcc cgcgctccgc tgccagtcgg
     9781 ccatctcggc gaacgaccag gtgccggccc tgctgaggaa ggcgaagtag aggtcgaaca
     9841 gcccgccgat ctgccccccc tcccccggcc gctcgcggcg gatcacctcc aggatcacca
     9901 ggacgccgcc cggccgcagc gaccgggcga tccgctcgac gaggccgcgg ttcgccgcct
     9961 cgccgaagtg gtgcgcgagc tggaagagga gcaccacgtc ccaggccccc gcccccagtt
    10021 tgtccgccat cgcgtcgccg gcgcggtgga cgacgcgctc ccccatcgcc tccttggcga
    10081 ggagcggcgc cgactgctcg atggcggcgg ggaggtccag caccaccccc cgcaacccgg
    10141 ggtggcggcg gcagagcgcc accg
//
LOCUS       DASPJI010000814         2924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_795998, whole genome shotgun sequence.
ACCESSION   DASPJI010000814 DASPJI010000000
VERSION     DASPJI010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795998"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..467
                     /locus_tag="VEL74_22975"
     tRNA            392..467
                     /locus_tag="VEL74_22975"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:425..427,aa:Ala,seq:ggc)
     gene            694..1071
                     /locus_tag="VEL74_22980"
     CDS             694..1071
                     /locus_tag="VEL74_22980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG65464.1"
                     /translation="MQPRKVLVVDDSKLMHKMYEVMLRQYPLAYASDGRQALDRLQEH
                     SDIDLVILDINMPNMNGLEFLARIRADQAFRDLPVVIISTEGRDEDTAQGLAAGATAY
                     IKKPFQSEQILEVISRLEKGPAA"
     gene            1068..1856
                     /locus_tag="VEL74_22985"
     CDS             1068..1856
                     /locus_tag="VEL74_22985"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HYG65465.1"
                     /translation="MSDRSSHATAESRLASPAIPGLDLAVELRSALDRAGELARTLEL
                     NLREASERGDSPGSSDERAVELERRLATAESDVKELASRLVDSEHQGGRLMNLYVATY
                     QLHATLDPAEVQATIAEIAINLLGAERFVLLLRRDEGEGCEIALIEGMHDEMDPLYAG
                     ETYTGGDPMVDTTLTDGVLRLGPTPESQALAAVPLRVQNEIVGALVLLKLFDHKPILR
                     AEDRDLLDLLSAHAASALFAARLFATKDRKLRTLESLVKLARGE"
     gene            1886..>2924
                     /locus_tag="VEL74_22990"
     CDS             1886..>2924
                     /locus_tag="VEL74_22990"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HYG65466.1"
                     /translation="MSISGSLKDVSVADVMQFIHLGRRTGTLLLARGSERSMIGFHCG
                     KLVSAQAPRTPKLGDLLISSGMIDRGILESAIREQNNGLERRSLGQILVGSGSIDAEG
                     LRQVIARQIEQAVSEVMVWDTGTFEFAIDDLRPIDDIALYPSDVLPDADINTQMVLLE
                     AARIFDERNRDRAEGYEEGDSTHPTMLDSGTEPQFALMERGETALEPREPEPIHAAER
                     LQLHIVTSDTDFADLAAEALAAEVAWAGVKAPGQAGLAGPGEPPPIILVDLRQGEMPL
                     EQAASLRRSRPRASLVALVDSGTPFAKVYEAGVLSAIPAEVEAVVACVENVIESRRDL
                     VRGARPGEPKAG"
BASE COUNT          481 a         1015 c          970 g          458 t
ORIGIN      
        1 ggagcgtccc attgaactca tacatggtct gcagggtgta cggatctcct tccccctgga
       61 cctgctcctg ggcgagggcc gccatcggcg cgcagatcat caacaccgcc ggaagccaga
      121 acccgagttt ccggttcatt ctcctgtcac tcctttccca aaacgacgaa gggcatcacg
      181 gtatcttatt caaagaatgg gccaacaagc tcccgaaccg gggcttccac ggtcgagagg
      241 ctcgcggcct cggtcacgat ctgagtcctt gtcctctgaa gagtgtcacg aaacggcaaa
      301 agcagtcctc ccccgcgaag gtcgccgact ttttcttcac gaggggtttg acatggggga
      361 cggcaggggc tacactccct gcctccagag tggggccgta gctcagttgg gagagcgctt
      421 gaatggcatt caagaggtcg agggttcgac tcccttcggc tccaccatct tccttatccc
      481 ctttcctctt cgcgtaggcc tgcggaatcc ggcggcggag ccaacgatga ccctggcggg
      541 ctttccttga atcgccccct gccattggct acactcacct ctcggactga gtggcaggca
      601 gtgcgacggg tccactgggg acctgagggt cccggggcag atctatgcgt aatttctcgg
      661 ggccgatgac cctttctgac gaggcggagt gccgtgcagc cacgtaaggt cctggtcgtc
      721 gacgactcga agctgatgca caagatgtac gaggtgatgc tgcggcaata cccgctggcc
      781 tacgcgtcgg acgggcgcca ggccctggac cggctccagg agcacagcga catcgacctc
      841 gtcatcctcg acatcaacat gccgaacatg aacggcctgg agttcctcgc gcggatccgc
      901 gccgaccagg ccttccgcga cctgccggtg gtcatcatca gcaccgaagg ccgggacgag
      961 gacaccgccc aagggctggc cgccggagcg accgcctata tcaagaagcc gttccagagc
     1021 gagcagatcc tagaagtgat ctcgcgcctg gagaagggac ccgcagcttg agcgaccgga
     1081 gcagccacgc aaccgccgag agccgtctcg cctctccggc catcccgggc ctcgacctgg
     1141 cggtggagct gcgttctgcc ctcgaccgtg ccggtgagct ggcccgtacg ctggagctaa
     1201 atctgcgcga ggcgtccgag cgcggggact cccccggctc gtcggacgag cgggccgtcg
     1261 agctggagcg gcggctggcg accgcggagt cggacgtcaa ggagctggct tcgcgcctgg
     1321 tcgactcgga gcaccagggc gggcggctga tgaatctgta cgtcgccacc tatcagctcc
     1381 acgccacgct ggacccggcc gaggtgcagg ccaccatcgc cgagatcgcc atcaacctcc
     1441 tcggagcgga gcggttcgtc ctgctgctgc gccgggacga gggcgagggg tgcgagatcg
     1501 cgctgatcga agggatgcac gacgagatgg acccgctcta cgccggcgag acctacaccg
     1561 gcggcgaccc gatggtggac acgaccctga ctgacggcgt cctccggctc gggccgaccc
     1621 cggagtcgca ggcgctggct gcggtgccgc tgcgggtgca gaacgagatc gtcggcgccc
     1681 tggtcctcct gaaactgttc gaccacaagc cgatcctccg ggccgaggac cgcgacctcc
     1741 tggacctgct ctccgcccac gccgcctccg ccctcttcgc ggcgcggctg ttcgccacca
     1801 aggaccgcaa gctccgcacc ctggagagcc tggtcaagct ggcgcgcggc gagtagagcc
     1861 cttcccgcgc gccccgggca aaccgatgag catctccggc agcctcaaag acgtctccgt
     1921 cgccgacgtg atgcagttca ttcacctcgg ccggcgcacc ggcacgctcc tcttggcgcg
     1981 cgggtcggag cggtcgatga tcggcttcca ttgcggcaag ctggtctcgg cccaggcgcc
     2041 gcgcacgccc aagctgggcg acctgctcat ctcctccggc atgatcgacc gcggcatcct
     2101 cgagtcggcg atccgcgagc agaacaacgg gctggagcgc cggtccctgg ggcagatcct
     2161 ggtcggctcc ggctcgatcg acgccgaggg gctgcgacag gtgatcgcgc ggcagatcga
     2221 gcaggcggtc tcggaagtga tggtctggga caccggcacg ttcgagttcg ccatcgacga
     2281 cctgcgtccc atcgacgata tcgccctcta cccgagcgac gtgctccccg acgccgacat
     2341 caacacccag atggtgttgc tcgaggcggc gcgcatcttc gacgagcgga accgcgaccg
     2401 cgccgagggg tacgaggagg gggattcgac gcatccgacg atgctcgatt ccggcaccga
     2461 gccgcaattc gcgctgatgg agcgcggcga gacggccctc gagccgcggg agccggagcc
     2521 catccacgcc gccgagcggc tgcagctcca catcgtcacc tccgacaccg acttcgccga
     2581 tctggcggcc gaggccctgg cggccgaggt cgcctgggcc ggagtgaagg cgccggggca
     2641 ggcgggcctt gccggaccgg gggagccgcc gcccatcatc ctggtggacc tgcggcaggg
     2701 tgagatgccc ctggagcagg cggcgtcgct gcggcgctcg cggccacgcg cctccctcgt
     2761 cgccctggtc gactccggta ccccgttcgc caaggtctac gaggccggcg tgctctccgc
     2821 catccccgcc gaggtcgagg cggtggtggc ctgcgtcgaa aacgtcatcg agagccggcg
     2881 cgacctggtc cgcggcgccc ggccggggga gcccaaggcc ggcg
//
LOCUS       DASPJI010000815         4963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_506866, whole genome shotgun sequence.
ACCESSION   DASPJI010000815 DASPJI010000000
VERSION     DASPJI010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506866"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VEL74_22995"
     CDS             complement(<1..215)
                     /locus_tag="VEL74_22995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65467.1"
                     /translation="MTVRRLRRAVAMGVLAVALAVSSAEAAPAAARFAAPDLWAQAWE
                     WVASWWGAWSGEARPSGEKQGGTSESGG"
     gene            595..1002
                     /locus_tag="VEL74_23000"
     CDS             595..1002
                     /locus_tag="VEL74_23000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65468.1"
                     /translation="MNATQKTAVQTQEAAADKKLIRPRGEKLRQARSEKLKAERVQEK
                     LAADRFTKRLQTVPGWNLTEDHQALYRQRQFPDTASAASFALHAMQLVLGQGQGLSLS
                     ALSGQVVVLLHGVPQGGGYRLAEDTLDLAERLG"
     gene            1056..1445
                     /locus_tag="VEL74_23005"
     CDS             1056..1445
                     /locus_tag="VEL74_23005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65469.1"
                     /translation="MATGSFGESLSGMEQLAANAEPRVTAHPLLQDIHSELRNALTEV
                     RDVKTRQLEAQATAQAVTKEVVAALTRARDLAARLRAGLTTLFGKHDERLVEFGIKPI
                     RRRPRKGPIETPVEDAPTEPDLDTDVN"
     gene            1718..1888
                     /locus_tag="VEL74_23010"
     CDS             1718..1888
                     /locus_tag="VEL74_23010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65470.1"
                     /translation="MKRLLFAGAITLTLFASRPAQALVICRHFDCMSVCTFYDDEGSF
                     MKKEFIPTYCSM"
     gene            2504..3904
                     /locus_tag="VEL74_23015"
     CDS             2504..3904
                     /locus_tag="VEL74_23015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65471.1"
                     /translation="MGSTSDSRLWAAFSYRHQFHEYSSSGRLLTEIIVEGGEVREVED
                     AEARRQQAAQTEAELAKEGIRSRVVEVTAKPVGIAVSEGRDGRMYFLVHGGSGTAGSQ
                     ISLERFDPARVILERVSLPIPAPGRGSMASGSDGLYIAPFQPKNGLWRIPWEDLDAAE
                     WEPVQGVEIGISDPSKLEGVWREKGGRGLVRFAKDKVITWEDGRLTVRGLVSLEGREA
                     LLRRSGFGESWKVALRTGGLRIERDGTGREYTRLAEIPKSVSLDPAPLGKAKELAPER
                     VAAIRADLEQRVRRAAEARKKTDLNDWRLAVADNLRYLGELTAEIGWIDARRFGVQAS
                     SQAATLAQDGSDVLLMLAALPFLEKDLKPVPDGAPVYSGFYDALQVALGGKQRYGTQI
                     VPDRAGEPVVLPIESRAQVDPIRKKIGLEPLADFLALTSRSLFQGKTIRIAEGEVVEA
                     PKAAAAPAGAAAVNSR"
     gene            4095..4577
                     /locus_tag="VEL74_23020"
     CDS             4095..4577
                     /locus_tag="VEL74_23020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65472.1"
                     /translation="MSHSHPSNAFTAGFLARLGERDEPPTAGEAAVAGPWRIEPVPGK
                     GFGLFRAGQTAGRGGRPAALCPSRWTALVTAAVLPGTGRDPLLRLGKEADAEGFYPVT
                     LDTGEVVMRLDVFDDTLIEHVNVLIHLLRSPRSLADALEAGGAEALGACGAILDERLP
                     "
BASE COUNT          873 a         1662 c         1640 g          788 t
ORIGIN      
        1 ccgcccgatt ccgaggtgcc gccctgcttc tcgccggagg gccgggcctc cccggaccag
       61 gcgccccacc aggaagccac ccactcccag gcctgagccc agagatccgg agcggcgaag
      121 cgggccgccg ccggcgccgc ctcggccgaa gacaccgcca acgccaccgc cagaaccccc
      181 atggcgacag cacgccgcaa acgcctcaca gtcatctcca tcctcctctg ccgtggaccg
      241 gcccggggcc tccatggcct cgagcccgcg aatcccggcc atcgtccctt tcctactcca
      301 gtgccgctcg ggacgctttc cgggaaaccg aagctgtcag acagagcata ccctgaacaa
      361 tcttctcttt gaccacgcac gcatacgcat accagaagat tgcagcgaca aagatcgagt
      421 cgaggtattg acacctgaat ctacagcctg tataacttcg ttgacgatgc agtcagcggc
      481 accgaccgta agtgcaccag gggacgcccg gttcgtcatc aagctcagcc gatctccccg
      541 gaccgtcatg aaggcggccg gtttccgggt caaggacccg aggaggacaa cgcgatgaat
      601 gcaactcaga agactgccgt gcagacgcag gaggccgctg cggacaagaa gctgatccgg
      661 ccgcgcggcg agaagctccg ccaggcgcgg agcgagaagc tcaaggccga gcgggtgcag
      721 gagaagctcg cggccgatcg cttcacgaag cggctccaga ccgtgcccgg ctggaacctc
      781 accgaggacc accaggccct ctaccggcag cgccagttcc cggacaccgc gtcggcggcc
      841 agcttcgccc tgcacgccat gcagctcgtg ctgggccagg ggcagggcct gtcgctgtcg
      901 gccctctccg ggcaggtggt ggtgctgctg cacggagtcc cccagggcgg tggctaccgc
      961 ctggccgagg acaccctgga cctggccgag cggctcgggt gagccggcgg ccctgacaac
     1021 ctaacgcaac acaaaagaga aaggagaact ctcacatggc gacaggatca ttcggcgaaa
     1081 gcctcagcgg catggagcaa ctcgccgcga acgcggagcc ccgcgtgacg gcgcacccgc
     1141 tgctccagga catccacagc gagctcagga acgcgctcac cgaggtgcgg gacgtgaaga
     1201 cccgccagct cgaggcgcag gccacggcgc aggcggtcac caaggaggtg gtggcggccc
     1261 tgacccgggc gcgggacctg gcggcccggc tgcgcgccgg cctcaccacc ctcttcggca
     1321 agcacgacga gcggctggtc gagttcggga tcaagcccat ccggcgccgg ccgcggaagg
     1381 gcccgatcga gacgcccgtg gaagacgccc cgaccgagcc cgacctcgac actgacgtga
     1441 actgatgaca ccaaccggtg agggcgcgag gccacgcgcc ctcaccacct ccattctacc
     1501 gcgccgctgt cgatacgtcg ccggcggcgc cgttctttga caactggctt tacgggagcg
     1561 ccgccgtgcc caaggcctcg ctcccgcatg agaaggatag agaaaggcgg gggctggatg
     1621 cctccgcctt ccctcttttc gcgcaggtgg tgaaacaggg ctcggctcgg gcgtatactt
     1681 gtgggtcaac ccaggaggga aacgaatgcg accgagcatg aagcgactgc tgtttgccgg
     1741 agccatcacc ctgacgttgt ttgcctcacg gcctgcccag gctctggtca tttgccggca
     1801 tttcgactgc atgagcgtgt gcacttttta cgacgacgag ggtagcttca tgaagaagga
     1861 attcatcccc acgtactgct cgatgtgagt catgacgatg atgaggcgtg tcgcttgggt
     1921 ctggtcggct tggttgttcg gcgccagcct tgtaggcgcg gccgacttgg atagcgcggg
     1981 cacgcaggtc cgcccccagc atctggcgcc ctccagcacg gcttggagtg gccagcagtt
     2041 cgttcaagcg gatagcgggg ggcggatctt tttgctgaga gccgaggctc tcgaggttta
     2101 tacccttgag gcagacggtc agctcggtgg ggcccagcgg cttcagggat cctccagcgc
     2161 cggaccggct ctcgactcct ttgtgctcga tgcggcgatg tcaggctcgg gcgactgggt
     2221 gctgaatcag ggtcacgtgg tcagactttt ccgccggggc caggaggaat ccctgaatga
     2281 tcctcgttgg gatatcaccg gcctcggggt catcgggggg gaccctgtga tcgcggggac
     2341 ggggggggag gtcggggccc aactcttcct caagaaggag agcgcagacg cgactccgct
     2401 gctgctgacc tggcagggac aacaatggga gacattcggt tcgacttcgg agaagctggc
     2461 ggacccccaa ccgctcgagt tggaaatacg aagggcgttg cggttgggaa gcacctcgga
     2521 cagccgccta tgggccgcat tctcctaccg gcaccagttt catgagtaca gctcctcggg
     2581 ccgcctcctc acggagatca tcgtcgaggg tggagaggtt cgcgaggtcg aagatgccga
     2641 agcacgccgg cagcaagcgg cccagaccga ggcggagttg gccaaggaag gcatacggtc
     2701 acgcgtcgta gaggtgacgg ccaaacccgt gggcatcgcc gtcagcgaag ggcgggatgg
     2761 ccgcatgtac tttctcgttc acgggggctc gggaaccgcg ggctcgcaaa tcagtctcga
     2821 gcgcttcgat cccgcccgcg tcatcctgga gcgggtgtcg ttacccatac cggccccggg
     2881 cagaggctcc atggcctcgg ggtcggatgg cctctacatc gccccctttc agcccaagaa
     2941 cggcctctgg cgcatcccct gggaagacct cgacgcggcg gagtgggagc cggtccaagg
     3001 agtggagatc gggatatccg atccgtcgaa gctcgaaggc gtctggcggg aaaaaggggg
     3061 acgagggctc gtccgcttcg ccaaagacaa agtgatcacc tgggaggatg gccgcctcac
     3121 cgtccgcggg ctggtgagcc tggaggggcg ggaggcgctc ctgcgccgct ccgggttcgg
     3181 cgagagctgg aaagtcgccc tccggacagg ggggctccgc atcgaacggg acggcaccgg
     3241 ccgcgagtac acccggctcg ccgagatccc caagtcggtc agcctcgacc cggccccctt
     3301 gggcaaggcc aaggagctgg cgcccgagcg ggtcgcggcg atccgggcgg atttggagca
     3361 gcgggtacgc cgtgccgcgg aggcccgcaa gaaaaccgac ctcaacgatt ggcgcctcgc
     3421 cgtggccgac aacctccgct atctcgggga gctgaccgcc gagatcggct ggatcgacgc
     3481 ccgccgcttc ggggtgcagg cctcctccca ggcggccacc ctggcccagg acggctcgga
     3541 cgtcctcctg atgctcgccg cccttccttt cctggagaag gacctcaagc cggtgccgga
     3601 cggcgccccc gtctactccg gcttctacga cgccctgcag gtcgccctcg gcggaaagca
     3661 gcgctacggc acgcagatcg tcccggacag ggcgggcgag ccggtggtgc tgcccatcga
     3721 gagccgcgcc caggtcgacc ccatccgtaa gaagatcggc ctcgagccgc tcgccgactt
     3781 cctcgccctc accagccgct ccctgttcca gggaaagacc attcgcatcg ccgagggaga
     3841 ggtggtggag gcgccgaagg cagcggcggc gccggccggc gcggcggccg tcaattcccg
     3901 ctgaaatcct ctctcgaacg cttgcttttc gcttcgcgtt cttatagttt ccaggtgttg
     3961 cacatggcag gacgtggccc ggcatgagcc gagtcggtcc ggaagtgagg tggcactcac
     4021 gaccggacct ggccacgaac acaaggaggc aaatccttat gtgtggttgc acccatggta
     4081 gcagctcgtc cgaagtgtcc cattcccacc cttccaacgc cttcaccgcc ggctttctcg
     4141 cccgtctcgg cgagagggac gagccgccca cggccggcga ggccgcggta gccggtccct
     4201 ggcggatcga gccggttccc ggaaaaggct tcggcctctt ccgggccggc cagactgccg
     4261 gccgcggcgg ccggccggcg gcgctctgcc cgtcgcgctg gacggcgctg gtgacggccg
     4321 cggtcctccc cggcaccggc cgcgatcccc tgctccggct cggcaaggaa gcggacgcgg
     4381 agggcttcta tcccgtcacg ctggacacgg gcgaggtcgt gatgcggctc gacgtcttcg
     4441 acgacaccct gatcgagcac gtcaacgtcc tcatccatct gttgcggtcg ccgcgcagcc
     4501 tggcggacgc cctggaagcc ggaggcgcgg aagccctcgg agcctgcggc gccatcctcg
     4561 acgagcggct cccctgatcg aacccttcga agccgtggct tgctccgccg tcccgggagc
     4621 aggccacggc ttcaccttta gcatcgtggt tctccttttg catcgaagcc ctatcttgaa
     4681 tggtgccgag accgtcgccg tccggtttcg acaccgcgcc attccggaac caatgtaaag
     4741 atcatgatcg aaagcactga ataggaccgc atgcttgctt tttttgaccg caatccggta
     4801 gagtccccgc gcctgtcgga cccaatgagg aattctgaat gagccgtatg tttcgttatc
     4861 gcgcctgtgt gacctttcgg agcgtggagg gggagaagga agaggagttt ccggtggtcg
     4921 ccagaactcc gaacgaggcg agcgacatgg ccttcgccta cgc
//
LOCUS       DASPJI010000816        17810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_399858, whole genome shotgun sequence.
ACCESSION   DASPJI010000816 DASPJI010000000
VERSION     DASPJI010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17810)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17810
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399858"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..605)
                     /locus_tag="VEL74_23025"
     CDS             complement(186..605)
                     /locus_tag="VEL74_23025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65473.1"
                     /translation="MTELEETEYGYRVALADSADTGDAASLLQEIRRRLRGRTGGEFG
                     VLLDLRRTSALPAEAQEVLALSLAHLRRGGMTRQAVVVNSRIILLQARHLAREAGADE
                     ICRYFDSASQEDWERLAVDWLARSLEPGRTAPEPGEE"
     gene            930..2603
                     /locus_tag="VEL74_23030"
     CDS             930..2603
                     /locus_tag="VEL74_23030"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HYG65474.1"
                     /translation="MSRDRRIPTGGRSRPSAAPQRDSDSAMMGLLLVTGSVLAGFLLL
                     SAAVGTGGPRWPSFVFLFFGGAAATAYGFRVYPYFRKLERRLPEESERWLKLENRLIG
                     AASHLGAGDLVRASEQTRGLPERLARMAEGASESLSGLAREIQDRSIEVASAADSVNQ
                     TASDLAAGSSEQAASVVQITAAMEELARTAAQIADNAARQASLAARAETSGDAGSAAV
                     HEAVAGVEEVQKRIQAISSRADALGTRSKEIYRVLDLITEIAQETHILSLNAAIEAFA
                     AGAEGRRFSVVAEEVRRLAQRAQESVASVRHLLDEFAGSIRATIVATEEGSKEANRVF
                     ERSRAAAAAIGDLRRASGDTSRVARQISMATHQQNAASDEVVMTLREVSQVVQRMTGG
                     LKDLSGTADRLNSLGLNIQLLAQSFHLASPRSLKHLVEDWAKQIERAAGAGEMEGVLD
                     SLVQRVAFVEMAYFVDTSGYTVTLCYNRAITGGRQLLGASGAREFDMTQRPWYRAVAR
                     EQRTALTPPYESLQNGETCFTVGAPVLNREGSFIGVLAVDINVNGWTRI"
     gene            2613..3056
                     /locus_tag="VEL74_23035"
     CDS             2613..3056
                     /locus_tag="VEL74_23035"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HYG65475.1"
                     /translation="MEAFTDAKAWKAGNHLLVRAGGYVCALPLGSVRRVVRALPVHAL
                     PGARAELRGLAEFAGEPMPVLDLARLVQAPPGALPAQPVTVVVWAGPPDARSMVGLAA
                     DAALEMADVSPGTMVGGGGGLVRGEVGIGGEVVRVLDLEALGREP"
     gene            3053..3385
                     /locus_tag="VEL74_23040"
     CDS             3053..3385
                     /locus_tag="VEL74_23040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65476.1"
                     /translation="MSGSGFLLMRRGGSLWGIANSAVAGLERQDGGAYRIAAGAEPAL
                     LVADEILGVVPELRVWQSPVTRRFWPEPFRGLSIHGRQPVVVVDPGHPPVALRPQGED
                     VGETEGRD"
     gene            3357..4994
                     /locus_tag="VEL74_23045"
     CDS             3357..4994
                     /locus_tag="VEL74_23045"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HYG65477.1"
                     /translation="MLERQRDETSVANYARIGLLLMAGTVLMGLVMLLVSEAHGARSW
                     PFSLFLLLSVALLWLYGSIVYRLQLRLKGRIDSTVRRHTSVERTLAETLDRLRAGDLL
                     HCAEQAQSLPGRLAAAFANATNSLDLLAQQIQSSSIEVAGAASSVNQIASELASGSSQ
                     QAASVVEITAAMEELARTASEIAENAAIQAELAETAEDSGNSGAAAVEEAVAGVEEVM
                     KRISGIANRAETLGTRSREIYRVLDLITEISQETHILSLNAAIEAAAAGEHGRRFSVV
                     AEEVRRLAQRSQESVESVRNLLDEFAGSIRATVVATEEGGKEAARVLERGRAAAAAIE
                     ELRDASGDTARVAREISQATQQQNAASDEVVLTLKEVSHVVQRMADGLKQFSGTADRL
                     NQLGLAIQMLAQSFHVDSHHSLKHLAETWAAVVRRRLGNWEAIERLLEDLVHQKAFVE
                     CLYFFDGKGNQVALTVNRHVMGDREVPPAVKAGEGFSERIWYKTATREGRTVLTPLFN
                     SLLSGELIFTAATPIYDRGELVGVLGLDVNVDSWAKI"
     gene            5016..7154
                     /locus_tag="VEL74_23050"
     CDS             5016..7154
                     /locus_tag="VEL74_23050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG65478.1"
                     /translation="MDPEFADLVPLFVEEARDRLDRLATSVPRLESDPQATVEAKREL
                     HTLKGAVRMLRLSPLAELCHAAEEVLHAPGPGIAPLMTRVVDELSAMVEAVARGEEPA
                     ADPELLRRLAAGPAPGPGPAAAPPAGLSAASLAPVPEDGHPLEPAAAAPRAAASPTAA
                     APPGRGAAVDIRVDAASLDAVAERATQMRINALSTRPVVERIYDLARLAEEGVREPQP
                     TQVLAVLATMLRRVAVELEGGQRRLLRSAEEQVDKMLALQLQPLRGPLLSLARYARDL
                     ARSLQREVEVELEGEDTRLDRRIARELEEALLHVVRNAVDHGIESPEVREARGKPRAG
                     RLQIRASAEGPRVRLVIADDGGGIDPVRVIQRAVEAGLVDRAAAAGFGKAEALRLLFA
                     PGFSTRKAVSEISGRGIGLDVVASVVNRVGGEVFLDSETRQGTTTILEVPVARRGEQV
                     VLLRVGQIRLAIPSAGLRRATWLPADAVVERDGRTLALLGERLVSFVPLARLYGQPAA
                     ESQLLLEGVVSGQQIALAVDEVEGEQEVLVRPIPPGIGADRLLEGIALLASGEPVGVL
                     SPSVLAQREYLRALPVQPEAPVAHRVRVLLVDDSLVTREMERRLLEDAGFEVSAAGDA
                     EEALTRLTEQTFDCMVTDIEMPGMDGFELAAHLRGMERYAHLPIVVVSTRDRPEDRLR
                     GLKSGADAYLTKQSLDGGELVDLVRRLGGR"
     gene            7184..8239
                     /gene="cheB"
                     /locus_tag="VEL74_23055"
     CDS             7184..8239
                     /gene="cheB"
                     /locus_tag="VEL74_23055"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HYG65479.1"
                     /translation="MSAIRVLLVDDSRSVRAVLKRFFARTPDIEVVGEAPDGERAVEA
                     VLATEPHAVVMDLQMPVMDGYAAIERIMAVRPTPIVVLSSRANRNQMETAFEAMRRGA
                     LEVLPKPEDTESWELLAGSLPDTVREVAAVRARPRRRRRPQPDGAPAPAGKGEPMAPR
                     PLRWVAIGASTGGPAAIREFLEEVPAHPPVSVLVVQHIAAGFEVGFADWLGKELRLDV
                     RLAQDGEEPRPGAVRVAPGGSHLVLEPGGTLHLDARTPARGSHRPAVDELFFSCAAAC
                     PHEVAGVLMTGMGADGAEGLAALRRAGGLTLVQDEASSVVFGMPRAALERGGAELSMP
                     PRTLARTLVHLWQEGER"
     gene            8236..9696
                     /locus_tag="VEL74_23060"
     CDS             8236..9696
                     /locus_tag="VEL74_23060"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HYG65480.1"
                     /translation="MSVHPPRRVLVVDDSEVTRAILSRTLRAAGFEVLEARDGVEGAL
                     TALREQPAVVLTDLEMPVLDGFPLLRLLKTDPVSAHIPVLILTSHAEAASRFWGLRTG
                     ADAYLTKDYRPGDLVATVERLIALAPAAPPPAPPQTPAAARKPELGPLDVLARVARQL
                     EASLLQATLLNTLMEGGMAAPGFHEASCAALQTVGSVVDARLLAVAMAEEQSTMLHVA
                     LEEPLHRLVVEDLRDRVLQRLGGGTAAAVDLQMSGETGTRQADLDGTVWFSLSFRNAR
                     GVMALLPRDPQQFAAGSAPLVEGLAGHLALVLDNARLSQRLRELSTLDGLTGQLNQRA
                     IHDRLTHELERAGRYRQALTVILCDLDHFKQVNDTHGHLAGDTVLREGAAVLRRGLRA
                     CDLLGRYGGEEFLAVLPQVDIDAGRMAAERLRRELEGYHVILPTGERVRITASFGVAS
                     RDELGGAAEVTGDHLVGLADRRLYQAKAAGRNRVRP"
     gene            9693..11075
                     /locus_tag="VEL74_23065"
     CDS             9693..11075
                     /locus_tag="VEL74_23065"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HYG65481.1"
                     /translation="MSLPPAIDKLVELIRERIGNVVPAARHGFLEQVAQRRAQATGYS
                     EVSQYVRALATGQLDREWDRLIPLITIKESYFFRAPQQFEIIRQQVLPRLRRVRSGAR
                     HLRVWSAACARGEEAGTLAMLLAGEKALEEWDWTILATDVDEEALAGARLGLYGERAV
                     AQVPPPLLERWFQRRGKLYELAPELRSRIVYQPLNLARPPYLLPFGELDLILLRNVLI
                     YFPRPLQKEVVSQAARSLAPEGYLFLGASETLWQIQDDLDPVDLGACFAYRHPSRPPV
                     ESRKEERTERKPGSSAAALPRPSSKLAETPATPPGIERAVKPPALAEPGGLHERLLGA
                     ARDLASNRIAEASLALEQVLAAEPSEPAAHALDGFLHDVTGRADEAVTAYRAALYLDP
                     ALYQVRVLLADCLLRLGHRDRAEQQYREVLTSLGSGRERDLLLFEDLPFPDRDRAQRR
                     SRQVLQGEGR"
     gene            11108..11923
                     /gene="amrB"
                     /locus_tag="VEL74_23070"
     CDS             11108..11923
                     /gene="amrB"
                     /locus_tag="VEL74_23070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HYG65482.1"
                     /translation="MSGRVRPAAVAGTFYPGSPGALRSTVEGLLAEAPPRNGAAKPPK
                     APKAIIAPHAGYLYSGPIAASAFQALAVPSRPISRVVILGPAHFVPIRGLALPGSEYF
                     ETPLGRVKVDPEGAGAVLRLPQVRVYPDAHVREHSLEVEIPFLQVLLGEGFSVVPLVV
                     GEADGEEVADVLERLWFEDDTVFVISSDLSHYLPYDEARRMDRMTADEILNLSGPLHP
                     RQACGAMPINGLLEAARRRGLAPELLDLRNSADTAGDPGRVVGYGAFAFHEGS"
     gene            11910..12476
                     /gene="amrA"
                     /locus_tag="VEL74_23075"
     CDS             11910..12476
                     /gene="amrA"
                     /locus_tag="VEL74_23075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HYG65483.1"
                     /translation="MREADVADERGRILLTIARASLGEALGLGPLRSFDEPWLREPGA
                     TFVTLRHRAKLRGCVGSLHAHRPLFDDVRANARAAAFHDTRFAPVSREELPELSFEVS
                     LLSSPAPFEVSCEEEALARLRPGVDGLIFDCEGCRSTFLPQVWDHLREPREFLTQLKL
                     KAGLPAAFWSSDVRLWRYTVSKWAEGEG"
     gene            12495..12917
                     /locus_tag="VEL74_23080"
     CDS             12495..12917
                     /locus_tag="VEL74_23080"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HYG65484.1"
                     /translation="MFRKILIPVDFTEKNEAALAAATQMLQGQDGGEAVLLHVVETIE
                     HLEFDEMSDFYRGLETRAAAKLSVMQEKLVASGVQARDEILFGKRAESVIRHAEDNGM
                     DLIVLSSHNVDRDHPALGGMGISYRIAIVARCPVLLVK"
     gene            12978..13988
                     /gene="gap"
                     /locus_tag="VEL74_23085"
     CDS             12978..13988
                     /gene="gap"
                     /locus_tag="VEL74_23085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HYG65485.1"
                     /translation="MGVRVGINGFGRIGRCTLKQFLDRDGCEVVGINDLADLGDLAYL
                     LKYDSIHGWYPPKVSEDGKSLRVGERQIPFFSQKDPAQLPWGDLGADVVIDASGAFRS
                     REKAAAHLAGGAKRVIISAPSDDADGTFVMGVNSDTYDPGRHVVISMASCTTNCLAPV
                     ARVLFDRFGVEHLMVTTVHAYTSSQALMDTPVRKRRRGRAAALSIIPTTTGAAKATEK
                     VIPELAGRMDGMAMRVPVPDGSICDIVTLLGQEVTAEQVNNALRQAADEPRFRGILRV
                     TGEALVSQDIVGDSHSSIIDAESTMVLRGRVAKVLSWYDNEWGYSARLVDFACFMGER
                     GV"
     gene            complement(13985..14299)
                     /locus_tag="VEL74_23090"
     CDS             complement(13985..14299)
                     /locus_tag="VEL74_23090"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HYG65486.1"
                     /translation="MVTSSSRRLKRHRHGAPGPHLPQAHLRRSAPAPGRRPPSRADRG
                     RTGILNERNASGAPDLVIEILSPGTRRVDETLKLQLYERAGVSEYWIIDPSSETARIR
                     LF"
     gene            complement(14696..15124)
                     /locus_tag="VEL74_23095"
     CDS             complement(14696..15124)
                     /locus_tag="VEL74_23095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HYG65487.1"
                     /translation="MSRAYIPKALRDRVAAQAKHRCGYCLTPSRIVGALLEIEHLVPE
                     SLGGPTEEENLWLACSPCNGHKGNRIAAIDPLTEKNVPLFDPRRQLWVEHFAWTSSGE
                     RIVALTATGRATVTALRLNRPSLVLARREWVSVGWHPPKD"
     gene            complement(15121..15552)
                     /locus_tag="VEL74_23100"
     CDS             complement(15121..15552)
                     /locus_tag="VEL74_23100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65488.1"
                     /translation="MDEEIIPMAAHAVTVHLPSPLYDQLRRQAEDAHRSVEAELLDVV
                     ANVVPMTAGLPDDLEAALSGLEILDDDALWRAARTTLPEEVSERLEELHFVERRRELT
                     EAESGELSALLRQYERTMLVRARAARLLKERGHDIDSLIRE"
     gene            15815..16333
                     /locus_tag="VEL74_23105"
     CDS             15815..16333
                     /locus_tag="VEL74_23105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65489.1"
                     /translation="MRDCNDGTRVSSQPSNVFTRDFLERLDEADEPATASEAEVAGVA
                     GVEPVSGGFGLFLTGESSERGHEPMAVFREQWRAKVAAAVLPGTGRDPLVVLRKEREA
                     SGFAVLAGLDGGGQPDVVGHLRTFDEAWASAMHVAEALLRSPDCLALFLKAAGKVALE
                     RTGTLLDRQVRD"
     gene            16464..17726
                     /locus_tag="VEL74_23110"
     CDS             16464..17726
                     /locus_tag="VEL74_23110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HYG65490.1"
                     /translation="MLRTIARTYRDAFSGLPRPVWLLSIASLVNRSGTMVLPFLALFL
                     TEERGFSTEGAGRALALYGVGAVTGSWLGGWLCDRFAPTRVMKWSLVGTGAGFLAVGQ
                     AEDRALVLALIVVVSIIGEAFRPAAQAAVAAACPPEVRTRGFALNRLAINVGMTLGPA
                     LGGFLALYNYAWLFVADGVTCLVAAFLVHLFFPTPLRTAALKNAAAPAGQAAARAAGS
                     PWRDLPFLGVMFLSFLMAAAVFQVFTTFPLTLRDLYGMREDSIGLALAVNTLVIVLFE
                     MVLVHSLDRRDPLEISGVGSFLTCLGLGLLPLGAVFGWGFGYVALSVMVWSVGEMLTL
                     PFLAGVVANRAGEGNLGRYMGFYVTTFSAAFVIAPLLGTWIYDTWGPHALWYGWGSLG
                     LPLWAGFHWLAVRERRSRGQMAVTVPAA"
BASE COUNT         2385 a         6310 c         6472 g         2643 t
ORIGIN      
        1 gtctacgccg ccatcatcgc ccgggttggc gcccggcttg ggggagcgct gaaataaaaa
       61 gagcccggca cgagaccggg ctgtggagga aggatgcggt cgtccgcgtc ctacggtgtc
      121 cgccggcccc gaaggaccga cagcaccgcg ccgtcaacta tagtgaaaaa acgcacgaaa
      181 atgtttcact cctctccagg ttccggcgcg gtcctccccg gttcgaggct ccgcgccagc
      241 caatcgacgg ccaatcgctc ccaatcctcc tggctcgccg agtcgaaata gcggcagatc
      301 tcgtccgccc ccgcctcgcg cgccaggtgg cgggcctgga gcagaatgat gcggctgttg
      361 accaccaccg cctgccgcgt catccctccc cgccgcaggt gagccaggct cagcgccagc
      421 acctcctgcg cctcggccgg cagcgcgctc gtgcggcgca ggtcgaggag caccccgaac
      481 tcccccccgg tccggccgcg gagccgccgc cggatctcct ggagcagcga cgccgcgtcg
      541 cccgtatcgg cggagtccgc cagcgcgacc cggtatccat actccgtctc ctccaactcc
      601 gtcatccgaa gaaacccctt tcacccccgg aacatgcaca ccaacaggca cgtcagacca
      661 ttttcaagta tagctttggt tataggactc ttccggaggg ggtggcggct tcgcggcccg
      721 agaggacccg tgcaggagtt gcaatatccc cgaagatcgg caaactttag tgcagctctc
      781 ggatttcccg gcttgcggcg tggggttgaa ttccctatcc tacgaccgcg ttcaatctgc
      841 aaagccggat ggagtttgcc ggactcgacg ccttcccggg gggcgcggct cacgagccgg
      901 acgggggctc gccggaagga gaggtcagga tgagccgaga caggaggatc cccaccggcg
      961 ggcggagcag gccgtcggcg gcccctcagc gggatagcga cagcgcgatg atgggcctgc
     1021 tgctggtgac cggctcggtg ctcgcggggt tcctgctgct gagcgcggcg gtcgggacgg
     1081 gcgggccgag gtggccgagc ttcgtcttcc tgttcttcgg cggcgccgcc gccaccgcct
     1141 acggtttccg ggtctacccc tatttccgca agctggagcg ccgcctgcca gaggagtcgg
     1201 agcgctggct gaagctggag aaccggctga tcggggccgc ctcccacctc ggggccggcg
     1261 acctggtgcg ggcgagcgag cagacccgcg gcctgccgga gcggctggcg cggatggccg
     1321 aaggggcgag cgagtcgctg tcggggctcg cccgcgagat ccaggaccgc tccatcgagg
     1381 tggcgtccgc cgccgactcg gtcaaccaga ccgcctccga cctcgcggcc ggctcgtccg
     1441 agcaggccgc gtcggtggtg cagatcaccg ccgccatgga ggagctggcg cgcaccgcgg
     1501 cgcagatcgc cgacaacgcc gcgcgccagg cctccctcgc cgcccgggcg gagaccagcg
     1561 gcgacgccgg ctcggccgcc gtgcacgagg cggtggccgg cgtcgaggag gtgcagaagc
     1621 gcatccaggc gatctccagc cgcgccgacg ccctggggac ccgctccaag gagatctacc
     1681 gcgtcctcga cctgatcacc gagatcgccc aggagaccca catcctgtcg ctcaacgccg
     1741 ccatcgaggc cttcgccgcc ggcgccgagg ggcggcgctt ctcggtggtg gccgaggagg
     1801 tccgccgcct ggcgcagcgt gcccaggagt cggtggcctc ggtgcgccac ctcctcgacg
     1861 agttcgccgg ctcgatccgc gccactatcg tcgccaccga ggaggggagc aaggaggcga
     1921 accgggtgtt cgagcgctcg cgcgccgccg ccgctgcgat cggcgacctg cgccgggcct
     1981 cgggcgacac ctcgcgggtg gcgcgccaga tctccatggc cacccaccag cagaacgccg
     2041 cctccgacga ggtggtgatg acgctgcgcg aggtgagcca ggtggtccag cggatgaccg
     2101 gcgggctcaa ggacctgtcg ggcacggccg accggctgaa cagcctgggg ctcaacatcc
     2161 agctcctggc gcagtcgttc cacctcgcct cgccgcgctc cttgaagcac ctggtggagg
     2221 actgggccaa gcagatcgag cgggccgccg gcgccggcga gatggagggg gtcctcgact
     2281 cgctggtgca gagagtggcg ttcgtcgaga tggcctactt cgtcgatacc agcggctaca
     2341 ccgtcacgct ctgctacaac cgcgcgatca ccggcggccg gcagctcctc ggggcgtcgg
     2401 gggcgcggga gttcgacatg acgcagcgcc cctggtaccg tgccgtggcg cgggagcagc
     2461 gcacggccct gacgcctccc tacgagtcgc tgcagaacgg cgagacctgc ttcaccgtcg
     2521 gcgcgcccgt gctcaaccgc gaggggtcct tcatcggtgt cctggcggtg gacatcaacg
     2581 tcaacggctg gacgcggatc taggggaccg gggtggaggc tttcacggac gcgaaggcct
     2641 ggaaggccgg taaccacctg ctggtgcggg cgggcggcta cgtctgcgcg ctgccgctgg
     2701 gctccgtccg ccgcgtcgtc cgcgccctgc ccgtgcacgc cctgcccggc gcgcgggcgg
     2761 agctgcgcgg cctcgccgag ttcgctggcg agccgatgcc ggtgctcgac ctcgcccgtc
     2821 tggtgcaggc gccgcccggg gcgctgccgg cccagccggt gacggtggtg gtctgggccg
     2881 gtccgccgga cgcccggagc atggtgggac tggcggcgga cgccgccctg gagatggccg
     2941 acgtctcccc cggcacgatg gtgggggggg gcggcggcct ggtgcgcggc gaggtgggga
     3001 tcggcggcga ggtggtgcgc gtgctcgacc tcgaagcgct gggacgggag ccatgagcgg
     3061 cagcggcttc ctcctcatgc gccgcggcgg cagcttatgg gggatcgcca acagcgccgt
     3121 ggccggtctc gagcgccagg acggcggcgc ctaccgcatc gcggccggcg ccgagccggc
     3181 gctcctggtc gccgacgaga tcctgggcgt ggtgccggag ctgcgggtct ggcagtcccc
     3241 ggtgacccgg cgtttctggc ccgagccgtt ccgcggcctg tcgatccatg gccggcagcc
     3301 ggtggtggtg gtggaccccg gacacccccc ggtggccctg cggccccaag gagaggatgt
     3361 tggagagaca gagggacgag actagcgtcg ccaactatgc ccgcatcggg ctcctgctca
     3421 tggcggggac ggtcctgatg gggctcgtga tgctcctggt gtcggaggcg cacggggcgc
     3481 ggagctggcc gttcagcctg ttcctgctgc tgtcggtggc gctcctctgg ctctacggca
     3541 gcatcgtcta ccggctgcag ctgcgcctca aggggcggat cgacagcacg gtgcggcgcc
     3601 acacgtcggt cgagcggacg ctggccgaga cgctcgaccg gctgcgcgcc ggcgacctgc
     3661 tgcactgcgc ggagcaggcg cagtcgctgc ccggccgtct cgccgcggcg ttcgccaacg
     3721 ccaccaactc cctcgacctg ctggcgcagc agatccagtc cagctccatc gaggtggccg
     3781 gggcggccag ctcggtgaac cagatcgcct cggagctcgc ctcgggctcg tcgcagcagg
     3841 cggcctcggt ggtggagatc acggctgcca tggaggagct cgcccgcacc gcctcggaga
     3901 tcgccgagaa cgccgccatc caggccgagt tggcggagac ggccgaggac agcggcaact
     3961 ccggcgccgc cgcggtggag gaggcggtcg ccggcgtcga agaggtgatg aagcggatct
     4021 ccggcatcgc caaccgcgcc gagaccctgg gcacccgctc gcgcgagatc taccgggtcc
     4081 tcgacctgat caccgagatc tcccaggaga cccacatcct gtcgctcaac gccgccatcg
     4141 aggccgccgc cgccggcgag cacggccgcc gcttctcggt ggtggccgag gaggtccggc
     4201 gcctcgccca gcgctcgcag gagtcggtcg agtcggtgcg caacctgctg gacgagttcg
     4261 ccggctcgat ccgcgccacc gtcgtcgcca ccgaggaggg gggcaaggag gcggcccggg
     4321 tgctggagcg cgggcgggcg gcggccgcgg ccatcgagga gctgcgcgac gcctcgggcg
     4381 acaccgcccg cgtggcgcgg gagatctcgc aggccaccca gcagcagaac gccgcctccg
     4441 acgaggtcgt gctcaccctc aaggaggtga gccacgtggt gcagcgcatg gccgacggcc
     4501 tcaagcagtt ctcgggcacc gccgaccgcc tgaaccagct cggcctcgcc atccagatgc
     4561 tggcgcagtc gttccacgtc gactcgcacc actcgctcaa gcacctggcc gagacctggg
     4621 ccgccgtggt ccgccgccgg ctgggcaact gggaggcgat cgagcggctg ctggaggacc
     4681 tggtgcacca gaaggcgttc gtcgagtgcc tctacttctt cgacggcaag gggaaccagg
     4741 tggcgctcac cgtcaaccgc cacgtcatgg gcgaccgcga ggtgccgccg gcggtcaagg
     4801 cgggggaggg gttcagcgag cgcatctggt acaagaccgc cacccgcgag gggcggacgg
     4861 tcctcacccc cctgttcaac tccctgctct cgggcgagct catcttcacc gccgccaccc
     4921 cgatctacga ccgcggggag ctggtggggg tcctggggct cgacgtcaac gtggatagct
     4981 gggccaagat ctaggcccgt cccgctcgga gccccgtgga cccggagttc gccgatctcg
     5041 tcccgctctt cgtagaggag gcgcgcgacc ggctggaccg gctcgccacc agcgtgccac
     5101 gcctggagtc cgacccccag gccacggtgg aggccaagcg cgagctccac accctcaagg
     5161 gggcggtgcg catgctccgc ctctcgcctt tggccgagct ctgccacgcc gccgaggagg
     5221 tgctgcacgc gcccggcccc ggtatcgcgc cgctgatgac ccgcgtcgtc gacgagctgt
     5281 cggcgatggt ggaggcggtc gcccgcggcg aagagccggc ggccgatccc gagctgctgc
     5341 ggcgcctcgc cgccggcccg gcgcccggcc ccggccccgc cgccgcgccg cccgccggtc
     5401 tatccgccgc cagcctggcg ccggtgccgg aggacggcca tcccctggaa ccggcggctg
     5461 ccgcgccccg ggccgcggcc tctcccacgg ccgcggcgcc gcccggccgt ggcgccgcgg
     5521 tggacatccg ggtggacgcc gcctccctgg acgccgtggc cgagcgggcg acgcagatgc
     5581 ggatcaacgc gctctccacc cgtccggtgg tggagcgcat ctacgacttg gctcgcctcg
     5641 ccgaggaggg ggtgcgcgag ccccagccga cgcaggtgct ggccgtcctc gccaccatgc
     5701 tgcggcgggt ggcggtcgag ctcgaagggg ggcagcggcg gctcctgcgg tccgccgagg
     5761 agcaggtgga caaaatgctg gcgctccagc tccagccgct gcgcgggccg ctcctgtcgc
     5821 tcgcccgcta cgcccgggac ctggcgcggt cgctgcagcg cgaggtcgag gtcgagctcg
     5881 agggggagga cacccggctc gaccgccgca tcgcccgcga gctggaggag gcgctgctgc
     5941 acgtcgtccg caacgccgtg gaccacggca tcgagtcgcc ggaggtgcgg gaggcgcgcg
     6001 gcaagccgcg cgccgggcgg ctgcagatcc gcgcttcggc cgaggggccg cgggtgcgcc
     6061 tggtgatcgc cgacgacggc ggcgggatcg acccggtgcg ggtgatccag cgggcggtcg
     6121 aggccgggct ggtcgaccgc gccgccgccg ccggcttcgg caaggccgag gccctgcgcc
     6181 tgctcttcgc gcccggcttc tcgactcgca aggcggtgtc ggagatctcc ggccgcggta
     6241 tcggcctcga cgtcgtcgcc tcggtggtga accgggtggg cggcgaggtc ttcctggact
     6301 ccgagacgcg ccaggggacc accaccatcc tggaggttcc ggtcgcccgc cgcggcgagc
     6361 aggtggtgct gctgcgcgtc gggcagatcc ggctggccat cccgtctgcc gggctgcgcc
     6421 gcgccacctg gctgccggcg gacgcggtgg tggagcgcga cggccgcact ctggcgctcc
     6481 tgggcgagcg gctcgtctcc ttcgtccccc tggcccgcct ctacggccag cctgccgccg
     6541 agtcccagct cctcctcgaa ggggtggtct cgggccagca gatcgccctg gcggtggacg
     6601 aggtcgaggg ggagcaggag gtgctggtgc ggccgatccc gccagggatc ggcgccgacc
     6661 ggctgctcga agggatcgct ctcctcgcct cgggcgagcc ggtgggggtg ctctcgccgt
     6721 cggtgctggc gcagcgcgag tacctgcgcg ccctgccggt ccagcccgag gcgccggtgg
     6781 cgcaccgcgt gcgggtgctg ctggtggacg actccctggt cacccgcgag atggaacgcc
     6841 ggttgctgga ggatgccgga ttcgaggtct cggcggccgg ggatgccgag gaggccctca
     6901 cccgcctgac cgagcagacc ttcgactgca tggtgaccga tatcgagatg ccggggatgg
     6961 acggtttcga gctggccgcg cacctcaggg gtatggagcg ctatgcccac ctgcccatcg
     7021 tcgtggtctc cacccgcgac cgtcccgagg accggctgcg cggcctcaag tccggcgccg
     7081 acgcctatct gaccaagcag agcctggatg gtggcgagct ggtggacctg gtccgccggc
     7141 tgggcgggcg gtgaccggga tggcttcgga tacagtctcg tccatgtccg cgatccgcgt
     7201 gctcctggtg gatgactccc gttccgtgcg cgcggtgctg aagcggttct tcgcccgcac
     7261 cccggacatc gaggtggtgg gggaggcgcc ggacggggag cgggcggtcg aggcggtgct
     7321 cgccaccgag ccccacgccg tggtcatgga cctccagatg ccggtcatgg acggctatgc
     7381 cgccatcgag cggatcatgg cggtgcggcc gacgcccatc gtcgtcctct cctcccgcgc
     7441 caaccgcaac cagatggaga ccgccttcga ggcgatgcgg cgcggcgccc tggaggtgct
     7501 ccccaagccg gaggacaccg agagctggga gctgctcgcc ggcagcctgc ccgacacggt
     7561 gcgggaggtg gcggcggtac gggcccggcc gcggcggcgc cgccggcccc agccggatgg
     7621 cgcgcccgcg ccggccggaa agggcgagcc catggctccc cgtcccctgc gctgggtggc
     7681 gatcggcgcc tcgaccggcg gcccggcggc aatccgcgag ttcctggagg aggtcccggc
     7741 ccacccgccg gtgagcgtcc tggtggtgca gcacatcgcc gccggcttcg aggtgggctt
     7801 cgccgactgg ctgggcaagg agctgcggct ggacgttcgg ctggcgcagg acggcgagga
     7861 gccgcggccg ggggcggtgc gcgtcgcccc gggcggctcc catctggtcc tcgagcccgg
     7921 cggcacgctc cacctggacg cccggacgcc ggcccggggc tcgcaccggc cggcggtgga
     7981 cgagctgttc ttctcctgcg ccgccgcctg cccccacgag gtggccggcg tgctgatgac
     8041 cggcatgggg gccgacggcg ccgaggggct cgcggcgctg cgccgggccg gcgggctcac
     8101 cctggtgcag gacgaggcca gctcggtggt cttcggcatg ccgcgggcgg ccctggagcg
     8161 cggcggcgcc gagctctcca tgcccccccg caccctcgcc cgcaccctgg tccacctctg
     8221 gcaggagggg gagcgatgag cgtccatcct ccgcgccggg tcctggtggt ggacgacagc
     8281 gaggtgacgc gggcgatcct ctcgcggacc ctgcgcgccg ccggcttcga ggtgctggag
     8341 gcgcgcgacg gcgtcgaggg ggcgctcacc gccctccggg agcagccggc cgtggtcctc
     8401 accgacctcg agatgccggt cctcgacggc ttccccctgc tgcggctgct gaagaccgat
     8461 ccggtgagcg ctcatatccc ggtcctgatc ctcaccagcc atgccgaggc ggcctcccgc
     8521 ttctgggggc tgcgcaccgg cgccgacgcc tacctgacca aggactaccg tccgggggac
     8581 ctggtcgcca ccgtcgagcg cctcatcgcc ctcgccccgg ccgctccgcc gccagcaccg
     8641 ccccagacac cggccgcggc acggaagccg gagctgggcc cgctcgacgt gctggcccgg
     8701 gtggcgcgcc agctcgaggc gagcctgctc caggccaccc tgctcaacac cctgatggaa
     8761 ggagggatgg cggcccccgg cttccacgag gcgagctgcg cggccctgca gaccgtcggc
     8821 tcggtggtgg acgcccgcct gctggccgtg gccatggccg aggagcagtc gaccatgctt
     8881 cacgtcgccc tggaggagcc gctgcaccgg ctggtagtgg aggatctgcg cgaccgcgtg
     8941 ctgcagcgcc tgggaggagg gacggccgcg gccgtcgacc tgcagatgag cggcgagacc
     9001 ggcacccgcc aggccgacct cgacgggacc gtctggttct ccctgtcgtt ccgcaacgcc
     9061 cgcggggtga tggcgctgct gccgcgcgac ccgcagcagt tcgccgccgg ctcggcgccg
     9121 ctggtcgagg ggctcgccgg ccatctggcc ctggtgctgg acaacgcccg cctctcccag
     9181 cgcctgcgcg agctgtccac cctggacggg ttgaccgggc agctcaacca gcgcgccatc
     9241 cacgaccgcc tgacccacga gctggagcgc gccggccgct accggcaagc gctgacggtg
     9301 atcctctgcg acctcgatca cttcaagcag gtcaacgaca cccatggcca tctggccggg
     9361 gacacggtgc tgcgggaggg ggcggcggtg ctgcgccggg ggctgcgggc ctgtgacctg
     9421 ctcggccgtt acggcggcga ggagttcctg gcggtactcc cccaggtgga catcgacgcc
     9481 gggcggatgg ccgcggagcg cctgcgccgc gagctcgagg gctatcacgt gatcctgccg
     9541 acgggcgaga gggtacggat caccgcctcg ttcggggtcg cgtcgcgcga cgagttgggc
     9601 ggggcggcgg aggtcaccgg cgaccacctg gtgggcttgg ccgaccgccg gctctatcag
     9661 gccaaggcgg caggccgcaa ccgcgtccgt ccatgagcct gccgccggcc atcgacaagc
     9721 tcgtggagct gatccgggag cggatcggca acgtggtccc cgccgcccgc cacggcttcc
     9781 tcgagcaggt ggcgcagcgg cgggcgcagg ccacgggcta ttccgaagtc tcccagtacg
     9841 tgcgggcgct ggccaccggc cagctcgacc gggagtggga ccgcctgatc ccgctcatca
     9901 ccatcaagga gtcgtacttc ttccgcgccc cccagcagtt cgagatcatc cggcagcagg
     9961 tgctgccgcg gctgcgccgc gtccgctccg gggcccgcca cctgcgcgtc tggtcggcgg
    10021 cctgcgcccg cggcgaggag gcggggacgc tggccatgct gctggcgggg gagaaggcgc
    10081 tcgaggagtg ggactggacg attctcgcca ccgacgtgga cgaggaggcg ctggccggcg
    10141 cgcggctggg gctctacggt gagcgggccg tggcgcaggt gccgccgccc ctgctggagc
    10201 gctggttcca gcgccgcggc aagctctacg agctcgctcc ggagctgcgg tcgcgcatcg
    10261 tctaccagcc gctgaacctc gcccggccgc cctatctgct gccgttcggc gagctcgacc
    10321 tgatcctgct gcgcaatgtc ctcatctact tcccccggcc gctgcagaag gaagtggtga
    10381 gccaggccgc ccgctccctc gcccccgagg ggtacctgtt cctgggcgcc tccgagaccc
    10441 tgtggcagat ccaggacgac ctcgacccag tggacctggg agcctgcttc gcctatcgcc
    10501 accccagccg gccgccggtg gagagccgga aagaggagag aacggagaga aagccgggga
    10561 gctccgccgc cgctctcccc cgtccatcgt ccaagctcgc ggagacgcct gccacccctc
    10621 caggcatcga gagggcggtc aagcccccgg ccctggccga gcccggcggg ctccacgagc
    10681 ggctactggg agccgcgcgg gacctggcgt ccaaccggat cgccgaggcc tcgctcgccc
    10741 tggagcaggt gctggcggcg gagccttccg agcccgccgc ccacgccctg gacggcttcc
    10801 tgcacgacgt caccggccgc gccgacgagg cggtcaccgc gtaccgcgcc gccctctacc
    10861 tcgaccccgc cctctaccag gtgcgggtgc tcctcgccga ctgcctgctg cggctcggcc
    10921 accgcgaccg ggcggagcag cagtaccgtg aggtgctcac cagcctgggc agcggccggg
    10981 agcgcgacct gctgctcttt gaggatctcc ccttccccga ccgggaccgg gcccagcgcc
    11041 gctcgcggca ggtgctgcag ggggagggga ggtagcgccg gccggacgta cgcggtacat
    11101 tgggcccatg tcgggccgag tccgccccgc cgccgtcgcc ggcacgttct acccggggtc
    11161 tcccggggcg ttgaggtcca ccgtcgaggg gctcctcgcc gaggccccgc cacgaaacgg
    11221 agccgcgaag ccgccgaaag cgccgaaggc gatcatcgcg ccgcacgccg gctacctcta
    11281 ctccggtccc atcgccgcct cggcgttcca ggcccttgcc gtcccgtccc gtccgatctc
    11341 ccgggtggtc atcctggggc cggcgcactt cgtgccgatc cggggcctgg cccttcccgg
    11401 gtccgagtac ttcgagacgc cgctggggcg ggtgaaggtc gatcccgagg gggccggcgc
    11461 cgtgctgcgg ctgcctcagg tgcgggtcta tcccgacgcg cacgtgcgcg agcactcgct
    11521 ggaggtggaa atccctttcc tccaggtgct tctcggggag ggcttctccg tcgtccccct
    11581 ggtggtgggg gaggcggacg gcgaggaggt cgccgacgtc ctggagcggc tctggttcga
    11641 ggacgacacc gtcttcgtca tcagctcgga cctctcccac tacctgccct acgacgaggc
    11701 ccggcggatg gaccgcatga cggccgacga gatcctgaac ctgagcggcc ctctgcaccc
    11761 acgccaggcc tgcggcgcca tgccgatcaa cggcctcctg gaggcggccc gccggcgcgg
    11821 gctcgctccc gagctgctcg acctgcggaa ttcggcggac accgccggag accccggccg
    11881 ggtggtcggc tacggcgcct tcgccttcca tgagggaagc tgacgtggcg gacgagcgcg
    11941 gccgaatcct cctgaccatc gcgcgggcga gcctggggga ggcgctgggg ctgggcccgc
    12001 tccgctcctt cgacgagccc tggctacggg agcccggggc cacattcgtg accctccgcc
    12061 accgtgcgaa gctgcggggc tgcgtgggct cgctccacgc tcaccggccg ctcttcgacg
    12121 acgtgcgcgc caacgcccgt gccgcggcct tccacgacac ccgcttcgcc ccggtgtcgc
    12181 gggaggagct cccggagctg tccttcgagg tgtcgctcct gtcgtccccg gcgccgttcg
    12241 aggtgagctg cgaggaggag gccctggccc gcctgcgtcc cggcgtcgac ggcctcatct
    12301 tcgattgcga gggctgccgc agcaccttcc tgcctcaggt ctgggaccac ctgcgggagc
    12361 cgcgcgagtt cctgacccag ctcaagctca aggccggcct ccccgccgcc ttctggtcct
    12421 ccgacgtccg cctctggcgc tacacggtga gcaagtgggc ggagggagag gggtagattt
    12481 ttcgaggagg gctcatgttc cgcaagatcc tgattccagt cgacttcacc gagaagaacg
    12541 aggcggccct ggcggcggcc acacagatgc tccaggggca ggacgggggc gaggccgtgc
    12601 tgctgcacgt cgtcgagacc atcgaacatc tggagttcga cgagatgagc gacttctacc
    12661 gcggcctgga gacgcgggct gcggcgaagc tgtcggtgat gcaggagaag ctggtggcgt
    12721 cgggagtcca ggcccgtgac gagatcctct tcggcaagcg cgccgagtcg gtcatccgtc
    12781 acgccgaaga caacggcatg gacctgatcg tcctctcctc ccacaacgtc gaccgcgacc
    12841 atcccgccct ggggggcatg ggcatcagct accgcatcgc catcgtggcg cggtgcccgg
    12901 tgctgctggt gaagtagggg gcacgggctt cgcaggggag gggggcggac cggacgagcg
    12961 aagaaaggag cggtgtcatg ggggtacgtg tcggcatcaa cggattcggg cggatcgggc
    13021 ggtgcacgct caagcagttc ctggaccgcg acgggtgtga ggtggtgggg atcaacgacc
    13081 tggcggacct gggcgacctg gcctacctgc tgaagtacga ctcgatccac ggctggtatc
    13141 cgccgaaggt ctcggaggac ggcaagtcgt tgcgcgtcgg cgagcggcag atccccttct
    13201 tctcgcagaa ggacccggcg cagctcccct ggggggacct gggggcggac gtggtcatcg
    13261 acgcctcggg tgcgttccgt tcgcgggaga aggcggcggc gcacctggcg ggcggggcga
    13321 agcgggtgat catctcggcc ccctcggacg acgccgacgg caccttcgtc atgggggtga
    13381 actcggacac ctacgatccc ggccggcacg tggtgatctc gatggcgtcg tgcaccacca
    13441 actgcctggc gccggtggcg cgggtgctct tcgaccgctt cggagtggag cacctgatgg
    13501 tcaccacggt gcacgcctac acctcgagcc aggcgctgat ggacacgccg gtgcgcaagc
    13561 ggcggcgggg ccgggcggcg gcgttgtcga tcatccccac gacgacgggg gcggccaagg
    13621 cgacggagaa ggtgatcccc gagctcgccg gccgcatgga cggcatggcg atgcgcgtcc
    13681 cggtgccgga cggctccatc tgcgacatcg tgacgctgct gggccaggag gtgacggcgg
    13741 agcaggtgaa caacgccctg cgccaggcgg cggacgagcc gcgcttccgc ggcatcctgc
    13801 gggtcaccgg cgaggccctg gtgtcgcagg acatcgtcgg cgactcccac tcgtcgatca
    13861 tcgacgccga gagcaccatg gtgctgcgcg gccgggtggc gaaggtcctc tcctggtacg
    13921 acaacgaatg gggctactcc gcccgcctgg tcgacttcgc ctgcttcatg ggggagaggg
    13981 gagtctagaa gaggcggatg cgggcggtct ccgaagaggg atcgatgatc cagtattcgg
    14041 agacgcccgc ccgctcgtag agctgcagct tcaaggtctc atcgacccgt cgggtgcccg
    14101 gggagaggat ctcgatcacc agatccgggg caccactggc gttccgctcg ttgaggatgc
    14161 cggtgcgtcc ccgatcagct cgtgacggag gccgtcgtcc gggagctgga gcagatcttc
    14221 gtaggtgagc ctgcgggaga tggggtccgg gagcgccatg gcgatgcctt ttcagccttc
    14281 ggctggaaga ggttaccact tcccgcgccg ggaagcggcg gggattctgg atctctggcg
    14341 gatgaccatc cctcctgggg cggtcgctgc cccaggagaa acggtggtca ttcctcgtgg
    14401 ggcaaccatt gccccatcag aaaggggcgc cattcctgat ggggcggcga ttgccccggc
    14461 aggaacgatc gccattcctc ctggggcagc gactgccccg tgaggaacgg tctccattcc
    14521 agtagaaacg gacaccattc ctcgtggggc agccattgcc ccgacaggaa tgacggccat
    14581 tcctcccggg gcagcggttg ccccaagagg aacgaggcct gttttctctc ggtttgtggt
    14641 cccagagggg gggtatcccc ctggactcgg ctgaggttgg tgacagcctt aaccttcaat
    14701 ccttcggcgg atgccaaccc accgagaccc actcccgacg ggccagcacc agggaagggc
    14761 gattgagccg caaggcggtg accgtggcgc ggccggtggc ggtgagcgcc acgatgcgct
    14821 ccccggagct cgtccaggcg aagtgctcga cccagagctg acggcgagga tcgaagaggg
    14881 ggacgttctt ttccgtcagg ggatcgatgg ctgcgatcct gttccccttg tggccgttgc
    14941 aaggagagca ggccagccag aggttctcct cctcggtagg gccgccgaga gactccggga
    15001 cgagatgctc gatctcgaga agggcgccca cgatccgcga tggggtcagg caatagccac
    15061 agcgatgctt cgcctgggcg gccacgcggt cgcggagggc cttcgggata taggcccggc
    15121 tcattcgcgg atcagagagt caatgtcgtg cccgcgctct ttcaggagac gggcggcacg
    15181 ggcgcggacc agcatggtcc gctcgtactg ccggaggagc gcggagagct cgccggattc
    15241 ggcctctgtc agctcgcggc gccgttccac gaagtgaagc tcttcgagcc gctcggacac
    15301 ctcttcgggc agggtggtcc gagccgcgcg ccagagggca tcgtcatcga ggatctccag
    15361 gccggagaga gcggcttcga ggtcgtcggg caaacctgcc gtcatcggga cgacgttcgc
    15421 gaccacatcc agcaactcgg cttcaacgct ccggtgggcg tcctcggctt ggcgccgaag
    15481 ctggtcgtag agagggctgg gcaggtgaac ggtgaccgca tgtgccgcca tcggaatgat
    15541 ctcctcatcc aaattttact ccaactgcac cctgccgcgc ccatctctct ctctaccgag
    15601 atgtcctcgg cccgccctcc gccccaaaag tttcccgcgc ccaagctcaa aatccctctt
    15661 gacccgggcg cacaagaggc gctaagatct ccagcgtcgt acccgtgcag tgccccgccg
    15721 gaggctgttc cggtccttac aacctgtgag acggcccggg gtgggtgtat cagacccaga
    15781 ccccgagcct gatcacgcaa ggagctgagt tcctatgcgc gactgtaacg atggtactcg
    15841 tgtctcttcc cagccgtcca acgtcttcac ccgggatttc ctggagcgcc tcgacgaggc
    15901 ggacgagccg gcgaccgcgt ccgaggcgga ggtggccggg gttgccggcg tcgagccggt
    15961 gtccggcggc ttcgggctgt tcctgacggg ggagtcctcc gagcgcggcc acgagccgat
    16021 ggcggtgttc cgcgagcagt ggcgggcgaa ggtggccgcc gccgtcctcc ccggcaccgg
    16081 ccgcgacccg ctggtggtcc tccgcaagga gcgcgaggcg tccggcttcg ccgtgctggc
    16141 cggcctcgac ggcggcggcc aacccgacgt cgtcggccac ctgcggacgt tcgacgaggc
    16201 gtgggcctcg gcgatgcacg tggccgaggc cctcctgcgg tcgccggact gcctggcgct
    16261 gttcctcaag gccgccggca aggtcgccct ggagcgcacc gggacgctgc tggaccggca
    16321 ggttcgggac tgagctctgg cgccccctcc cgccgcctgg atggggcggg agggggggct
    16381 tcggaaggtg agggcctccg cccacccgcg ccgttctaag ctaagcgctc cacatagagc
    16441 gcttacgata gagtcccccc gccatgctcc gcaccatcgc ccgcacctac cgcgatgcgt
    16501 tctccggcct gccccggccg gtgtggctgc tgtcgatcgc gtcgctggtg aaccggagcg
    16561 gcacgatggt gctgccgttc ctggcgctgt tcctcaccga ggagcggggg ttctccacgg
    16621 agggggcggg ccgcgccctg gccctctatg gcgtgggcgc cgtgacggga tcctggcttg
    16681 gcggctggct gtgcgaccgg ttcgccccga cccgggtgat gaagtggagc ctcgtcggca
    16741 ccggcgccgg cttcctcgcc gtgggacagg ccgaggaccg tgcgttggtg ctggcgctga
    16801 tcgtcgtcgt cagcatcatc ggcgaggcgt tccggcccgc cgcccaggcg gcggtggccg
    16861 ccgcctgccc gccggaggtg cggacccggg gcttcgccct caaccgcctg gcgatcaatg
    16921 tcggcatgac gctggggccc gccctcggcg gcttcctggc cctctacaac tatgcctggc
    16981 tgttcgtggc ggacggcgtc acctgcttgg tggccgcctt cctggtccac ctcttcttcc
    17041 cgacacccct ccgcaccgcg gccctgaaga acgccgcggc gccggctggg caggccgccg
    17101 cccgtgccgc gggctcgccg tggcgggacc tccccttcct gggggtgatg ttcctctcct
    17161 tcttgatggc ggcggcggtg ttccaggtct tcaccacctt cccgttgacc ctgcgcgacc
    17221 tctatggcat gcgggaggac tcgatcggac tggcgctggc ggtcaacacc ctggtcatcg
    17281 tcctcttcga gatggtgctg gtgcactcgc tcgaccggcg cgacccgctg gagatctcgg
    17341 gagtggggag cttcctgacc tgcctcgggc tcgggctgct gccgctgggc gccgtcttcg
    17401 gctgggggtt cggctacgtc gccctgtccg tcatggtctg gtcggtgggg gagatgctca
    17461 ccctgccgtt cctcgcgggc gtggtggcga accgggcggg ggaggggaac ctgggccgct
    17521 acatgggctt ctatgtcacc accttctcgg ccgctttcgt gatcgcgccg ctcctcggca
    17581 cctggatcta cgacacctgg ggcccccacg ccctctggta cggctgggga tccctcggcc
    17641 tccccctctg ggccggcttc cattggctgg cggtgcggga gcggcggagc cgcgggcaga
    17701 tggccgtcac ggtcccggcg gcctaaccct ccggcgtgtg ctcctccagc ttccggtagg
    17761 cccggttcca gtagatgagg ggcgggaggt ccggttgggg gacctggctg
//
LOCUS       DASPJI010000817        10313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_223775, whole genome shotgun sequence.
ACCESSION   DASPJI010000817 DASPJI010000000
VERSION     DASPJI010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10313)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10313
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223775"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VEL74_23115"
     CDS             complement(<1..954)
                     /locus_tag="VEL74_23115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65491.1"
                     /translation="MNRQLVVTGMHRSGTSLLANAASRAGFVMGSRLMAASKGNRHGH
                     FEDLDFVHFHERLLESRGWSALRPPAEPLVVSAEEEAEARALAAARAGLPSWGFKDPR
                     TTLVLDLWDRVLPAPVFLLVYRHPVEVALSLLRRGLDVEVQLDPGAAVQAWTVYNRRL
                     LEFLLAHRDRCLLWHVRGATGALTDAFGRLGERLGTSFPAAEVAAAFAPEDLRTGLWA
                     RGIDWPAVLPEAMELYARLEGAADLPGGPEPDPAERSSQPSQPSRPSPAERDLGEAAE
                     HLLAGALASSRPGAPLPAQLRVDYSELRLFAARQEERLAQLV"
     gene            1078..2061
                     /locus_tag="VEL74_23120"
     CDS             1078..2061
                     /locus_tag="VEL74_23120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012597081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65492.1"
                     /translation="MTRRSPRCRVLLAVLLASALGPAAAGCRPAAPFTVVRLGDGPIV
                     HAGTPGLQGELGASINGPSLIRVPSWVPDPLGRYYLYFAHHHGTFIRLAYADRLEGPW
                     TVHPGGVLPLAATAARHHVASPDVHVDAERRRILLYFHGPASETDRARQVTFLATSPD
                     GLHFTASPRELGPFYFRVFGHGGQRYALAKEGDRTGVLLRSADGETPFERGPEILPRM
                     RHAAVLVRGDVAWIVFSRLGDAPESLLFAPLRLTGDWRGWRPDEPVEVLRPERSWEGA
                     GEPVAASRSGRAWTPKQELRDPAFFVEDGRIYLLYAVAGEHGIALAEVRER"
     gene            complement(2069..4177)
                     /locus_tag="VEL74_23125"
     CDS             complement(2069..4177)
                     /locus_tag="VEL74_23125"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYG65493.1"
                     /translation="MKRPGYSLLLFLTGAATAAVAVAVQVAAWRLAGHPVPLALLGSH
                     LTVVLAVHTVAALLLARWRDGRPWALALLLVPALLAVLPAVDRWGGWRGAGLAAAGAA
                     ALWAAGSFRVRAPGRPLIASAVGAALGTSLASALLGLQLEAGPYTPGPVAKTVLVILL
                     AATLALLLGGERRLPGRSGLSPLPTPPAALGTAAAALALALAGSLTSLPAGLPEPARA
                     GGSPPPVVLIVLDTVRADHLRSYGYTRDTMPRLERFAREHAVRIEGALANAPSSLETH
                     ASMFTGLYPFRHGAHKPREGDPRPPLYAYPLESGPPTLATLLRRAGYWAVGVSANFGP
                     LAPAFGLDRGFAVYQARPGLQMGPARLDPYRFALWRRWPLAAADRLPPFARSEFFELG
                     APYRRAADIADQGIAALDASGEAPLFLFLNFMEAHYPYRPPRSFTRIFPGIRRPGLAR
                     LDDEQAAAAQVMAGRRPLTGAEREAWTAAYDSALRYLDGELGRLLDRLERHPRFPEML
                     VVITSDHGEALGEHGLLRHSTSLYQEMLRIPLFVKPGVRWSPGPPGPPGFQPGRAHPG
                     PFQPVDLFPTILQHAGVPIPAGIDGLVWGAGRRASFAECYPHPPARPLGERFRHEMRA
                     VVADGWKLILSTSGRAELYDLARDPGETTDLAAREPGRLARLRDMLASTFAGERRPRR
                     SPGPGGEALERSLRSLGYVQ"
     gene            complement(4174..4941)
                     /locus_tag="VEL74_23130"
     CDS             complement(4174..4941)
                     /locus_tag="VEL74_23130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65494.1"
                     /translation="MSLIDEALKRARQEAALQDAAQREAQFRRVPVYTPAPPSRSRNG
                     LLMAGVGICVGLVLALGLAFAAGWLPAGRSAEPVREELRAAAESPAPAPQIIEEAPSP
                     APAESAAPTAASPVPAPVPTPVPTPVTVEPQPAPLPETAPTPVLPATPEPREAEPAPV
                     EALAAVEAPAPVPAGEAAEPVEPLPSFSYVRQVPVPGGGTLELSGIAFSAERPVALIN
                     GRAWVPGEIVQGYTIVAVEPGRVQLEGHGQKVSVSLK"
     gene            complement(4938..5747)
                     /locus_tag="VEL74_23135"
     CDS             complement(4938..5747)
                     /locus_tag="VEL74_23135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYG65495.1"
                     /translation="MYNHFYGFLEAPFNITPDPRFLFLGDRHREAFHHILFGIRERKG
                     FIQLTGDVGAGKTTVCRAILEDLGPRYRTALILNPCLEPVELLRSVLTELGLQAPRDR
                     VRCLQVLNQFLLDQLAAGNDVVLLIDEAQDMSFELLEQVRLLSNLETDQRKLLQIVLI
                     GQPELREKLNDPALRQLRQRITVRYHLTPLTRPETETYIRHRIHVAGGNSRPTFTPWA
                     VRAIHRYAGGVPRLINAACDKALLCGYVEGTDVLTWRHVRRALRELEGEFS"
     gene            complement(5823..7889)
                     /locus_tag="VEL74_23140"
     CDS             complement(5823..7889)
                     /locus_tag="VEL74_23140"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HYG65496.1"
                     /translation="MHSMKVCLVTALASFLGAVPSAMADAEKKAPAANAETPYSVPVL
                     DPAAIDRGADPCVDFYQYTCGGWMKNNPTPEDRASWSRFDQLQRNNLLVLRGILEKAA
                     AGGAGRDANEQKIGDYYASCMDQKAIDARGIAPLKPELDRIAAIQDKTALARQLAHLH
                     LADVAALFGFGAEADFKNSTRVIAGVDQGGMGLPSRDYYLAEDAKSAELRQKYVDYAR
                     TLFQLLGDKPEQAAANAQTVMRIETALAKGALSPVERRDPSKVYHLMKVEELQQLAPS
                     FDWQTYFKAIEAPRFTELNVIEPEFFRQLDAQLRAVPLADWRTYLRYWLVNDLAPILP
                     AAFEKAHFDFYDRTLQGVQEMPPRWRSCVEYVDNDLGEALGREYVEVAFGEQARQRMD
                     ELITGLTTAMGEDIRGLDWMGAETKKQAQVKLSKFGTKIGHPKSWRDYGKLTVTRGDA
                     LGNWLRGNEFESRRQVAKIGTANDRDEWLMTPPTVNAYYHPLQNNINFPAGILQPPFF
                     DAKADDAVNYGAIGGVIGHEITHGFDDQGSQFDADGNLRNWWTDTDRKEFEERTSCLV
                     NQYSGYTAVDDVKVNGQLTLGENTADLGGTRIAYMALQKSLAGKEPQKIDGFTPDQRF
                     FIAWGQAWCENNRPEFARQMALTNPHAPGRYRANGIVSNTPEFHEAFGCKAGQPMVRE
                     NVCRVW"
     gene            complement(8018..9799)
                     /locus_tag="VEL74_23145"
     CDS             complement(8018..9799)
                     /locus_tag="VEL74_23145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65497.1"
                     /translation="MKSDRIESRCGRAVAAAWMAMALAAAAPAAAQPGIPFPIQPGQV
                     VVTCSAGDAPDGAVLAVFDIRNPAANAPGLNQNWLAPAYHAPNWTSANFNNGEVFGVT
                     LDDDTPPNIYATASSSYGGGRLGSGKVFRVDGTTGAVTVFADLPNCPAYPACPGSSYA
                     GLGNIAYDEERDQFFVTNFYDGKIYRLSASGAILEAFDFGAAFDPARNSAQGFVPLGE
                     RPWGVAVHDDRVYFAIWGRDYREGTANTVWSVGLDAAGAFEDFATQEVTVPTAGTGLS
                     MPVADISFSVEGRMLLAQRTMVNDFNPGARQSQVLEYTGGHLAWTPSTRLFEVGMVPR
                     TNAAGGADYLCEDQQTTGVAAMGDALHFGPGDFIFGLQIFPPGGGRIENSYLIDLDHN
                     TSTPDKFLVGSLDAHDVCGADVPPPTPWPDYDAIVTSGWNEDTRQKIPFGAADDDWQV
                     VSPGPVRPATVVLNPPPVEVWPRASKVAKWISSSASGQSSGPGTTEYRTCFCLSAQSK
                     DAMIMLKLRADDQATMFLNGRQLAGPGGQFRRAEPLEVNINDIVGQGFLQFGENCLTV
                     RVADRGNLTGFSIAGSFWVTDGLCVDE"
     gene            complement(9907..>10313)
                     /locus_tag="VEL74_23150"
     CDS             complement(9907..>10313)
                     /locus_tag="VEL74_23150"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HYG65498.1"
                     /translation="LLRRVLKGEELPAIHPLVDLNNCLSVILGVPSCTAAEGTFDPPV
                     TLRAGRDGESFESLRGPLGLAGKPLLADPAGPFGTPISDGERIRIRDETRRAWLALYL
                     PRGAVSAETARETLEGLLAEAPVAMVELIAVS"
BASE COUNT         1402 a         3555 c         3938 g         1418 t
ORIGIN      
        1 gacgagctgc gcgaggcgct cctcctgccg cgccgcgaac aggcgcagct cggagtagtc
       61 gacccgaagc tgggcgggca gcggggcccc cggccgggag gaggcgaggg cgccggccag
      121 caggtgctcg gccgcctcgc ccagatcgcg ctcggccggc gacggccgcg atggctgcga
      181 tggctgcgac gaccgctcgg cgggatcggg ctccgggccg cccggcaggt cggccgcccc
      241 ctccagccgg gcgtagagct ccatggcctc ggggaggacc gccggccagt cgatgccgcg
      301 ggcccacagg ccggtccgca ggtcttccgg cgcgaaggcc gccgccacct ccgccgccgg
      361 gaaggaggtg ccgagcctct cccccagccg gccgaaggcg tccgtcagcg cgccggtggc
      421 gccgcggacg tgccagagga ggcagcggtc ccggtgggcg aggaggaact ccaggagccg
      481 cctgttgtag accgtccacg cctggaccgc cgcgccgggg tcgagctgca cctccacgtc
      541 cagcccgcgg cgcagcagcg acagcgccac ctcgaccgga tgccggtaga ccaggaggaa
      601 gaccggcgcc ggcagcaccc ggtcccagag gtcgaggacg agggtggtgc gggggtcctt
      661 gaagccccag gaggggaggc cggcccgcgc cgccgcgagg gcgcgcgcct cggcctcctc
      721 ctccgccgac accaccagcg gctccgccgg ggggcgcagc gcgctccacc cgcgagactc
      781 caggagccgc tcgtggaagt ggacgaagtc caggtcctcg aagtggccgt gccggttccc
      841 cttgctggcg gccatcagcc ggctccccat gacgaagccg gcgcgcgaag cggcgttggc
      901 gaggagcgac gtgcccgagc ggtgcatgcc ggtgacgacg agctgacggt tcatgagggg
      961 acggtctccg gtgagccgcc ccttgtacca gaaggcgggg gccgcgtcca tacatcccgg
     1021 tgcatagacg cctcacctgc cttctggtac aaccgctgcc gtatccggag ccccgcgatg
     1081 acccgtcgat ccccccgctg ccgcgttctg ctcgccgtcc tgctggcctc cgcgctcggg
     1141 ccggcggctg ccggctgccg cccggcggcc ccgttcaccg tcgtccgcct cggcgacggg
     1201 ccgatcgtcc acgcgggcac gcccgggctc cagggggagt tgggggcgag catcaacggg
     1261 ccgtcgctga tccgggtgcc gtcgtgggtg cccgacccgc tgggccgcta ctatctctac
     1321 ttcgcccacc atcacgggac gttcatccgg ctggcctatg ccgaccgcct cgaggggccg
     1381 tggacggtgc acccgggcgg cgtcctcccc ctggccgcga cggcggcgcg ccatcacgtc
     1441 gcctcgccgg atgtccacgt ggacgccgag cggcggcgca tcctcctcta cttccacggc
     1501 ccggcctcgg agaccgaccg cgcccgccag gtcaccttcc tcgccacctc gcccgacggg
     1561 ctccacttca ccgcctcccc ccgggagctg ggccccttct acttcagggt cttcggccac
     1621 ggtggccagc gctacgccct ggccaaggag ggggaccgca ccggggtgct cctccgctcc
     1681 gcggacggtg agaccccctt cgagcgcggg ccggagatcc tgccgcgcat gcgccacgcc
     1741 gccgtgctgg tgcgggggga cgtggcctgg atcgtcttct cgcggctcgg ggacgcgccc
     1801 gagagcctgc tcttcgcccc gctgcgtctg accggcgact ggcggggctg gcggccggac
     1861 gagccggtgg aggtcctgcg gccggaacgg agctgggagg gtgcggggga gcccgtcgcc
     1921 gcctcccgca gtggccgcgc ctggacgccg aagcaggagc tccgcgaccc cgccttcttc
     1981 gtcgaggatg gccggatcta tctcctctac gccgtggccg gcgagcacgg catcgccctc
     2041 gcggaggtgc gggagcggtg aggccggcct actggacgta gccgagggag cgcagcgacc
     2101 gttccagcgc ctcgcccccc ggccccgggg agcggcgggg gcgcctctcg ccggcaaagg
     2161 tggaggccag catgtcccgg agacgggcga ggcgtccggg ctcgcgggcg gccaggtcgg
     2221 tggtctcgcc ggggtcgcgg gcgaggtcgt agagctccgc ccggccgctg gtgctcaaga
     2281 tcagcttcca gccgtccgcc accacggccc gcatctcgtg tcggaaacgc tcccccaggg
     2341 ggcgggccgg cgggtgggga tagcactcgg cgaaggaggc gcggcggccg gcgccccaga
     2401 ccaggccgtc gatccctgcg gggatcggca cgcccgcgtg ctgcagaatg gtagggaaga
     2461 ggtccaccgg ctgaaacggc cccgggtggg cccggccggg ctggaacccc ggcggccccg
     2521 gcggccccgg cgaccaacgt acccccggct tcacgaagag ggggatccgc agcatctcct
     2581 gatagagcga cgtcgagtgg cgcagcaggc cgtgctcgcc cagggcctcg ccgtggtcgg
     2641 aggtgatcac caccagcatc tcggggaagc gggggtggcg ctccagccgg tcgagcagcc
     2701 gcccgagctc gccatccagg tagcgcaggg cgctgtcgta ggcggcggtc caggcctccc
     2761 gttcggcgcc ggtgaggggg cgccggcccg ccatgacctg ggcggcggcc gcctgttcgt
     2821 cgtcaagccg cgccaggccc gggcgccgga tgccgggaaa gatccgggtg aaggagcgcg
     2881 gcggccggta ggggtagtgg gcctccatga agttgaggaa caggaagagg ggcgcctcgc
     2941 cgctcgcgtc gagcgccgcg atcccctgat cggcgatgtc ggcggcgcgg cgataagggg
     3001 cgcccagctc gaagaattcg ctcctcgcga acggcggcag gcggtcggcc gcggccaggg
     3061 gccagcgccg ccagagggcg aaccggtagg gatcgaggcg agccgggccc atctgcaacc
     3121 cgggccgcgc ctggtagacg gcgaatcccc ggtccagccc gaacgccggc gccagcggcc
     3181 cgaagttcgc cgacaccccc accgcccaat agcctgcccg gcgcagcagg gtggcgaggg
     3241 tgggggggcc gctctccagc ggatacgcgt agagcggcgg gcgcgggtcc ccctcgcgcg
     3301 gcttgtgggc gccgtgccgg aaagggtaga ggccggtgaa catcgacgcg tgcgtctcga
     3361 gcgagctggg ggcgttggcc agcgcccctt cgatccgcac cgcgtgctcg cgagcgaagc
     3421 gctctaggcg gggcatggtg tcccgggtgt agccgtagga gcgcaggtgg tccgcccgca
     3481 ccgtgtcgag gacgatgagg accaccggtg ggggcgaccc tcccgcgcgc gccggctccg
     3541 gaaggcctgc cggcagactc gtcaggctgc cggcgagcgc cagggccagg gccgcggcgg
     3601 cggtccccag ggcagccggt ggtgtcggga gaggggagag gccggagcgg ccgggcagcc
     3661 ggcgctcgcc gcccaggagc agggcgaggg tggcggcgag gaggatgacc aggaccgtct
     3721 tcgccaccgg gccgggggtg tagggaccgg cctccagctg gagccccaga agggccgatg
     3781 cgagggaggt cccgagcgcc gcccccaccg ccgaggcgat cagcggccgg ccgggagccc
     3841 ggacccggaa ggagccagcc gcccagagcg cggccgcgcc ggccgccgcc agcccggcgc
     3901 ccctccagcc gccccaccgg tccaccgccg gcaggacggc gaggagggcc ggcacgagga
     3961 gcagtgccag ggcccagggg cgtccgtccc gccaccgcgc gaggaggagg gccgccaccg
     4021 tgtgcacggc gagcaccacc gtcaagtggc tgcccaggag ggccaggggg accgggtggc
     4081 ccgccaaccg ccaagccgcc acctgtacgg cgacggccac cgccgcggtg gcggcgccag
     4141 tcaggaagag gaggaggctg tagcccggcc gcttcacttg agcgagacgg acaccttctg
     4201 gccgtgaccc tcgagctgca cgcggccggg ctcgacggcg acgatggtgt agccctggac
     4261 gatctcgccc ggcacccagg cccggccgtt gatcagcgcc accggccgct cggcggagaa
     4321 ggcgatgccg ctgagctcca gggtgccgcc gcccgggacc ggcacctgcc gcacatagga
     4381 gaaggagggg agcggctcca ccggctccgc ggcttcgcca gccggtacgg gcgcgggcgc
     4441 ctccacggcg gcaagagcct ccacgggcgc cggctcggcc tcccggggct ccggcgttgc
     4501 cggcaacacc ggggtggggg ccgtttccgg caggggagcg ggctggggct ccacggtgac
     4561 cggcgtgggc acgggagtgg gcaccggcgc cggcaccggg gaagccgccg tgggcgccgc
     4621 cgactcggcc ggagcggggg acggcgcctc ctcgatgatc tgcggcgcgg gggccggcga
     4681 ctcggcggcg gcgcggagct cctcgcggac cggctcggcg ctccggcccg cgggcagcca
     4741 gccggcggcg aaggcgaggc cgagcgccag gaccagcccg acgcagatcc ccacgccggc
     4801 catcagcagc ccgttccgcg agcgggacgg cggcgccggc gtgtagaccg gcacccgccg
     4861 gaactgggcc tcgcgctgag cggcgtcctg caacgccgcc tcctggcgcg cccgcttgag
     4921 cgcctcgtcg atcaggctca tgaaaactct ccttcgagct cgcgcagagc ccggcgcacg
     4981 tggcgccaag tcaggacgtc ggtcccctcc acgtagccgc acagcagcgc cttgtcgcag
     5041 gcggcgttga tcagccgcgg cacccccccg gcgtagcggt ggatggcccg caccgcccac
     5101 ggggtgaagg tgggccgcga gttgccgccg gccacgtgga tgcggtgccg gatgtaggtc
     5161 tcggtctccg gccgggtgag cggcgtcagg tgatagcgca cggtgatccg ctgccggagc
     5221 tggcgcagcg ccgggtcgtt cagcttctcc cgtagctccg gctggccgat gaggacgatc
     5281 tgcagcagct tgcgctggtc cgtctccagg ttggagagca gccgcacctg ctccagcagc
     5341 tcgaagctca tgtcctgcgc ctcgtcgatg agcagcacca cgtcgttgcc tgcggcgagc
     5401 tggtcgagca ggaactggtt cagcacctgg aggcagcgta cccggtcgcg cggtgcctgg
     5461 aggccgagct cggtgaggac cgagcgcagc agctcgaccg gctccaggca ggggttgagg
     5521 atcagcgccg tccggtaccg cggcccgagg tcttcgagga tcgcccggca gaccgtggtc
     5581 ttgcccgcgc cgacgtcgcc ggtgagctgg atgaacccct tgcgctcccg gatgccgaac
     5641 agaatgtggt ggaacgcctc gcggtggcgg tcgcccagga agaggaagcg cggatcgggc
     5701 gtgatgttga acggcgcttc gaggaagcca tagaagtggt tgtacatcgc aggaccgctt
     5761 cgattgtcgc acggagcggg gccggcggac cggccccgct ccctcggcga gtcagcgagg
     5821 gctcaccaga cccggcagac gttctcgcgc accatcggct gcccggcctt gcagccgaag
     5881 gcctcgtgga actccggggt gttcgacacg atgccgttgg cccggtagcg gccgggtgcg
     5941 tgcgggttcg tcagcgccat ctggcgggcg aactcgggcc ggttgttctc gcaccaggcc
     6001 tgaccccagg cgatgaagaa ccgctggtcc ggggtgaagc cgtcgatctt ctggggctcc
     6061 ttgcccgcga ggctcttctg gagcgccatg taggcgatcc gggtgccccc gaggtcggcg
     6121 gtattctcac ccaaggtgag ctggccgttg accttgacgt cgtcgaccgc ggtgtagccc
     6181 gagtactggt tcaccaggca ggaggtgcgc tcctcgaact ccttgcggtc ggtgtccgtc
     6241 caccagttcc gcaggttgcc gtcggcgtcg aactggctcc cctggtcgtc gaagccgtgg
     6301 gtgatctcgt ggccgatgac gccgccgatg gcgccgtagt tgaccgcgtc gtccgccttg
     6361 gcgtcgaaga acggcggctg caggatgccg gccgggaagt tgatgttgtt ctgcaacggg
     6421 tggtagtagg cgttgaccgt cggcggcgtc atcagccact cgtcgcggtc gttcgcggtg
     6481 ccgatcttgg cgacctggcg gcgggactcg aactcgttgc cgcgcagcca gttgcccagg
     6541 gcgtccccgc gggtcaccgt gagcttgccg tagtcgcgcc agctcttggg gtggccgatc
     6601 ttggtgccga acttcgacag cttgacctgg gcctgcttct tggtctccgc ccccatccag
     6661 tcgaggccgc ggatgtcctc tcccatggcg gtggtgaggc cggtgatcag ctcgtccatg
     6721 cgctggcgcg cctgctcgcc gaacgccacc tcgacgtact cgcggcccag cgcctcgccc
     6781 aggtcgttgt ccacgtactc gacgcaggag cgccagcgcg gcggcatctc ctgcaccccc
     6841 tggagcgtgc ggtcgtagaa gtcgaagtgg gccttctcga aggcggcggg gaggatcggg
     6901 gcgaggtcgt tgaccagcca gtagcgcagg taggtgcgcc agtcggccag cgggacggcg
     6961 cggagctgcg cgtccagctg ccggaagaac tcgggctcga tgacgttgag ctcggtgaag
     7021 cgcggcgcct cgatcgcctt gaagtaggtc tgccagtcga acgaaggggc gagctgctgg
     7081 agctcctcga ccttcatcag gtggtagacc ttcgacgggt cgcgccgttc gacgggggac
     7141 agggcgccct tggccagggc ggtctcgatc cgcatcaccg tctgcgcgtt ggcggcggcc
     7201 tgctcgggct tgtcgcccag gagctggaac agggttctgg cgtagtccac gtacttctgg
     7261 cgcagctcgg cggacttcgc gtcctcggcg aggtagtagt cgcggctggg cagccccatg
     7321 cccccctggt cgacgccggc gatcacgcgc gtggagttct tgaagtcggc ttcggcgccg
     7381 aagccgaaga gcgccgccac gtcggcgagg tggagatggg cgagctgccg cgccagcgcc
     7441 gttttgtcct gaatggccgc gatacggtcg agctccggct tcagcggggc gatgccgcgg
     7501 gcgtcgatgg ccttctggtc catgcacgag gcgtagtagt cgccgatctt ctgttcgttg
     7561 gcgtcccgcc ctgcgccgcc ggccgcggcc ttctccagga tgccgcgcag gaccagcagg
     7621 ttgttgcgct ggagctggtc gaagcgcgac caggaggcgc ggtcctcggg ggtggggttg
     7681 ttcttcatcc acccgccgca ggtgtactga tagaagtcga cgcaggggtc ggcgccgcgg
     7741 tcgatcgccg ccgggtcgag cacggggacg ctatacgggg tctccgcgtt ggctgccggg
     7801 gccttcttct ccgcgtcggc catcgccgac ggtaccgcgc ccaggaatga agcgagcgcg
     7861 gtgacgagac agaccttcat tgaatgcatc ggtatcctca ctgtccattg tccagggggt
     7921 gaccccgcgc ccgcggggcc cgaccaagct gtctatgcta acacccgccc ccgccccgag
     7981 cttccggggc ggggagggtg tcgatggagg ggggtgtcta ctcgtcgacg cagaggccgt
     8041 cggtgaccca gaacgagccg gcgatgctga agccggtgag gttgccgcgg tcggccaccc
     8101 gcaccgtcag gcagttctcg ccgaactgca ggaagccctg gccgacgatg tcgttgatgt
     8161 tgacctcaag cggctcggcg cggcggaact ggccgccggg cccggcgagc tgccggccgt
     8221 tcaggaacat cgtggcctgg tcgtccgccc gcagcttcag catgatcatg gcgtccttgg
     8281 actgggcgct caggcagaag caggtgcggt actcggtggt gccggggccg gacgactggc
     8341 ccgaggcgct ggaggagatc cacttcgcca ccttgctcgc gcgcggccag acctcgaccg
     8401 gcggcgggtt cagcaccacg gtggccggcc ggaccgggcc gggcgacacc acctgccagt
     8461 cgtcgtcggc ggcgccgaag gggatcttct ggcgcgtgtc ctcgttccac cccgaggtga
     8521 cgatggcgtc gtagtcgggc cacggggtcg gcggcggcac gtcggcgccg cagacgtcgt
     8581 gggcgtcgag ggagccgacg aggaacttgt cgggggtgct ggtgttgtgg tcgaggtcga
     8641 tcaggtagct gttctcgatc cggccgccgc cgggggggaa gatctgcagg ccgaagatga
     8701 aatcgcccgg cccgaagtgg agggcgtccc ccatggcggc gacgccggtg gtctgctgat
     8761 cctcgcacag gtagtcggcg ccgccggcgg cgttggtgcg cggcaccatg cccacctcga
     8821 acagccgggt gctgggggtc caggcgaggt ggccgccggt gtactccagc acctgcgact
     8881 gccgggcgcc cggattgaag tcgttgacca tggtgcgctg ggcgagcagc atccgccctt
     8941 cgacggagaa ggagatgtcg gccaccggca tggagaggcc ggtaccggcg gtggggaccg
     9001 tcacctcctg ggtggcgaag tcctcgaagg cgccggccgc gtccaggccc acggaccaga
     9061 ccgtgttggc ggtgccctcc cggtagtccc gcccccagat ggcgaagtag acccggtcgt
     9121 catggacggc gacgccccag gggcgctcgc cgagggggac gaagccctgg gcgctgttgc
     9181 gcgccggatc gaaggcggcg ccgaagtcga acgcctcgag gatcgccccg ctggcgctca
     9241 gccggtagat cttgccatcg tagaagttgg tgacgaagaa ctggtcgcgc tcctcgtcgt
     9301 aggcgatgtt gccgagcccg gcatagctgg agccggggca ggcggggtag gccgggcagt
     9361 tgggcaggtc ggcgaagacg gtgaccgcgc cggtggtgcc gtcgacccgg aagaccttgc
     9421 ccgagcccag ccggccgccg ccgtagctcg acgacgccgt ggcatagatg ttgggcgggg
     9481 tgtcgtcgtc cagggtgacg ccgaagacct cgccgttgtt gaagttggcg ctggtccagt
     9541 tgggtgcgtg ataggcgggg gccagccagt tctggttgag gccgggggcg ttcgccgccg
     9601 ggttgcggat gtcgaacacg gcgagaacgg cgccgtcggg cgcgtcgccg gccgagcagg
     9661 tgaccaccac ctggccgggc tgtatgggga aggggatgcc cggctgggcg gccgccgggg
     9721 cggcggctgc gagcgccatc gccatccatg cggcggccac agcccggccg cagcgggact
     9781 cgatccgatc agacttcatg ggaccctcct tgggtaacaa acggaacgga ctccggcaca
     9841 gcttctcagc aagaaaagtg ccagaagcct tcacccccgt ccgctctcac gggggggtgg
     9901 aggagctcag ctcaccgcga tcagctcgac catcgccacc ggcgcctcgg cgaggaggcc
     9961 ttccagggtc tcccgggcgg tctcggcgct caccgctccc cgggggaggt agagggccag
    10021 ccaggcgcgg cgggtctcgt cccggatccg gatccgctcg ccgtcggaga tcggcgtgcc
    10081 gaacgggccg gcggggtcgg cgagcagcgg cttgccggcc aggccgaggg ggccgcgcag
    10141 cgactcgaag gattcgccgt cgcggccggc gcggagggtc acgggaggat cgaaggtgcc
    10201 ttccgcggcg gtgcagctgg gtacgcctag gatcaccgag aggcagttgt tgaggtccac
    10261 cagcggatgg atggccggca gctcctcccc cttgagcacc cggcggagga ggg
//
LOCUS       DASPJI010000818         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_854154, whole genome shotgun sequence.
ACCESSION   DASPJI010000818 DASPJI010000000
VERSION     DASPJI010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854154"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3011)
                     /locus_tag="VEL74_23155"
     CDS             complement(<1..>3011)
                     /locus_tag="VEL74_23155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65499.1"
                     /translation="VALLILHHIICDGWSMGVLLREIAGLYTAFAPPSQEARPSPLPE
                     LGVQYADYAVWQRRWLTGEVLEAQLAYWRDELAGAPALLELPTDRPRPPVQTSHGAVE
                     PVVLTGELTAALRELGRREGITLFMALLAGFQALLHRLSGQDDVLVGSPLANRNRSET
                     EALIGFFVNTLVLRARFGSPEPEGLDVRTLLARARTAALNAYAHQDLPFERLVEELQI
                     ERSLAHGPLFQVMFALQNAPVGRLELPGLLLAPMDTGGGTAKFDLTLSFSESGGALAG
                     GLEYNTDLFDTATARRLAVQLEVLLAAAVADPGGRVWELPLLTAAQRSQLLVEWNDTA
                     SGYPRDLTLAELFAEQVARRPDAVALVSGGGGLEQVTYGELHRRATALSRRLAGLGAG
                     PEVPVALFAQRSPAAVAGLLAITMAGGFYVPLDPEHPAERLALLLADVGASLLLADRA
                     SAARLPATSAKLVLLDGEEAGGGEKAAPRLPRLPVPDNLAYVMYTSGSTGRPKGVGVT
                     HRNVNRLARGGRFAHMGSGQIFLQLAPLAFDASTLEVWGALANGGRLVLAPAGRLSLG
                     ELAETLALHEVTTLWLTAGLFHQVVEEAPEGLRHVRQLLAGGDVLSPPHVRRALAAMD
                     RGVLINGYGPTENTTFTCCHSMRRDEVGDERWMSSVPIGQPIADTRVYLLDSALQPVP
                     AGVYGELFAGGDGMARGYFGRPDLTAERFVPDPLGGSPGDRLYRTGDLARWRPEGVVE
                     FLGRRDHQVKLRGFRVEPGEVEAVLAGYPEVRQAAVLVGRDALGGKRLEGYVAGSVEA
                     PALRQHLESLLPPYMVPSVLVVLETLPLTPNGKVDRQALGRAGMDAAGVGGEGGEGGE
                     PRDVLELQLARIWREVLGVPRIGIRDDFFAAGGHSLLALSLMARVREQLGRDLPLAAL
                     FQAPTIESMAALLRRSEGEEAPAVSCLVPIQTQGSGRPFFCVHPGGGDVLCYAPLARY
                     LGPDRPFYGLQSRGLSGEAEPLGRIEDMAAL"
BASE COUNT          468 a         1072 c         1122 g          349 t
ORIGIN      
        1 gagggcggcc atgtcctcga tgcggccgag cggctcggcc tctcctgaaa gtccacggga
       61 ctggaggccg tagaaggggc ggtccgggcc caggtagcgc gccagggggg cgtagcagag
      121 gacgtctccg cctcccgggt ggacgcagaa gaacggccgg cccgagccct gggtctggat
      181 cggcacgagg caggagacgg ccggcgcctc ctccccctcg ctacgccgca gcagggccgc
      241 catggactcg atcgtgggcg cctggaagag ggcggccagc ggcaggtcgc ggccgagctg
      301 ctcgcgcacc cgggccatca gcgacagcgc cagcagggag tggccgccgg cggcgaagaa
      361 gtcgtcacgg atgccgatgc gcggcacccc cagcacctca cgccagatgc gggcgagctg
      421 cagctcgagg acgtcccggg gctcgccccc ctccccgccc tcaccgccga caccggcggc
      481 atccatcccg gcgcggccca gcgcctgccg gtccaccttg ccgttcgggg tgagagggag
      541 cgtctccagc acgaccagga ccgagggcac catatagggc ggcaagaggc tctcgaggtg
      601 ctgccgcaac gccggcgcct cgacactgcc ggccacgtag ccctccagcc tcttgcctcc
      661 cagggcgtcc cggcccacca gcaccgccgc ctgccggacc tccggatacc cggcgagcac
      721 cgcctcgacc tcccccggtt ccacccggaa gccgcgcagc ttgacctggt ggtcgcggcg
      781 gcccaggaac tcgacgacac cctccggccg ccagcgcgcg aggtcgccgg tgcgatagag
      841 gcggtcaccg ggggaaccgc cgagaggatc ggggacgaaa cgctccgccg tcaggtccgg
      901 ccggccgaag tagccgcgcg ccattccgtc gccgccggcg aagagctctc cgtagacgcc
      961 ggcgggcacc ggctgcagag ccgagtcgag caggtagacc cgggtgtcgg cgatcggctg
     1021 accgatgggc accgagctca tccatctctc gtcgcccact tcatcccgcc gcatggagtg
     1081 gcagcaggtg aaggtcgtat tctcggtggg gccgtagccg ttgatcagga caccgcgatc
     1141 catcgcggcc agggcgcgcc gcacgtgggg cggcgacagg acgtcgccgc cggccaggag
     1201 ctgccggacg tggcggagcc cttcgggcgc ttcttccacc acctgatgga agaggccggc
     1261 ggtgagccag agggtggtca cctcgtgcag ggcgagcgtc tccgccagct cgccgaggga
     1321 aaggcgaccc gccggcgcca ggaccagccg gccgccattg gcgagcgctc cccagacctc
     1381 cagtgtcgag gcgtcgaagg cgagaggggc tagctgcagg aagatctgtc cggaccccat
     1441 atgggcgaag cgcccgccgc gcgccagccg gttcacgttg cggtgcgtga cgcccacacc
     1501 cttgggccgg ccggtggagc ccgaggtgta catcacatag gccaggttgt cgggcacggg
     1561 gagccggggg agcctcggcg ccgccttctc ccctcccccg gcctcctcac cgtcgaggag
     1621 caccagcttg gcggaggtgg cgggcagccg ggcggccgac gcgcgatcgg ccagcagcag
     1681 ggaggccccg acgtcggcga gcaggagggc gagccgctcc gccggatgct cggggtccag
     1741 aggcacgtag aaaccgcccg ccatggtaat ggccaggagg ccggcgacgg ccgcggggga
     1801 gcgctgggcg aagagcgcca ccgggacctc gggtccggcg ccgagcccgg ccaggcggcg
     1861 ggagagggcg gtcgcccgcc ggtgcagctc gccataggtg acctgctcca gtccgccgcc
     1921 gccggagacg agggccaccg cgtccggccg gcgcgccacc tgttccgcga acagctcggc
     1981 gagggtgaga tcccgcgggt agccgctggc ggtgtcgttc cactccacca ggagctggga
     2041 tcgctgggcc gccgtgagca gcggcagctc ccagacgcgc ccgcctggat cggccacagc
     2101 agcggcgaga agcacctcga gctgcacggc gagccggcgc gcggtggcgg tgtcgaacag
     2161 gtcagtgttg tactccaggc ccccggccag agccccgccg ctctcgctga acgagagagt
     2221 gaggtcgaac ttggcggtgc cgccgccggt gtccatggga gcgaggagga ggcccggcag
     2281 ctccaaccgt cccaccggcg cgttctgcag ggcgaacatg acctggaaca gggggccgtg
     2341 cgcgaggctg cgctcgatct gcagctcctc caccaggcgc tcgaacggca gatcctggtg
     2401 ggcataggcg ttcaaggccg cggtgcgcgc ccgcgccagc agggttcgga cgtccaggcc
     2461 ctccggctcc ggggagccga agcgggcccg cagcaccagg gtgttgacga agaagccgat
     2521 gagcgcctcg gtctcgctgc ggttgcggtt ggccaaaggc gagcccacca gcacgtcgtc
     2581 ctggccagag aggcgatgga gcaaggcctg gaagccggcg agaagggcca tgaacagggt
     2641 gatcccctcg cgccggccca gctcgcggag agcggcggtc agctccccgg tcagcaccac
     2701 gggctcgacg gcgccgtggg acgtctggac cggcggccgc ggccggtcgg tgggcagctc
     2761 caggagcgcc ggggcgcccg ccagctcgtc gcgccaatag gcgagctggg cctccagcac
     2821 ctcgccagtg agccagcggc gctgccagac cgcgtaatcg gcatattgga cacccagctc
     2881 gggcagggga gacggccggg cttcctggga gggcggggcg aaggccgtgt agagccccgc
     2941 gatttcccgc agcaggacac ccatcgacca gccgtcgcag atgatgtggt ggaggatcaa
     3001 cagtgccacc c
//
LOCUS       DASPJI010000819         3161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_161910, whole genome shotgun sequence.
ACCESSION   DASPJI010000819 DASPJI010000000
VERSION     DASPJI010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161910"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..473
                     /locus_tag="VEL74_23160"
     CDS             69..473
                     /locus_tag="VEL74_23160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HYG65500.1"
                     /translation="MSDPILETPSGPTPTLAVCGCGTRHLHPDRGEGDEGEGRKALAV
                     DPQVKDKNLKRLRRIEGQVRGLHKMVEDDRYCADILTQISSVQEALRAVGRELMRNHL
                     KHCATAAIRSEEGQTAEAMYDELIDLIYKNSR"
     gene            complement(475..1134)
                     /gene="deoC"
                     /locus_tag="VEL74_23165"
     CDS             complement(475..1134)
                     /gene="deoC"
                     /locus_tag="VEL74_23165"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HYG65501.1"
                     /translation="MALPLARYVDHTLLRPDAMEEEVRSLITEAAEHGFASVCIPPVY
                     VPLAAAGLAGTGVHPGTVVGFPLGYVHPETRLAESRRAVADGAVELDTVLNISWLKSG
                     EDRRVLDDLAGWVRALRAEREGLVLKVILETCLLTDDEKVRGTRLVVEAGADFVKTST
                     GFAKGGATVEDVELLARTAEGRIGVKASGGIRDAVTARALIAAGATRLGTSSGVAIVR
                     G"
     gene            complement(1140..>3161)
                     /locus_tag="VEL74_23170"
     CDS             complement(1140..>3161)
                     /locus_tag="VEL74_23170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HYG65502.1"
                     /translation="ASDLEAITLPCCEEPLTAAVEGQALAVTGPLRIEPAAAAAQAGY
                     YRVQAAALRDERQAQELARRVARETGQPAEASFDAGIDLYRVRVGQYATREAAEQDLR
                     RLTLLGVTGAFVVSSGASVSDPALRVIQGGVPRTYPGRWLAVSSPSERGIRAQSRRYR
                     GRILVYLNDRGMLNLINELPVEQYLRGVVPSEMGPELYNQLEALKAQAVAARTYTLRN
                     LGEFAREGYDICATPRCQVYKGMDVEHPLSDRAIQETAGQVLLFEGRLVDALYSSTCG
                     GHTEDAPVVFPLKLEPYLEGVPCMEGGIHRLDGTLPAGVHFPEGLTQRLLPPTAGGSA
                     TRALAARLEHLALLAGLPAPRERLASLDRREVQRFVAAAFDLALDARLFLAPQDVEYL
                     LNDPPRDWSEDDLRRAAYLVKSGLLTGPLDQPLSDTEIEGMLLALAEMLRVVRREEVS
                     YLSHQGRRVAVKLDKEKEERTYDFPEQLATFRRQGDAVTSSDLALVTGDHLTLFWVGD
                     RLAAASQEVDLDGVAFDRSSPYSSWTRFRSDSQLAKQVNTRFPGLGFRDMEILSRGES
                     GRVGKIRIQGENGQVVEVEGLAIRWTLDVPDTLFTAKRLAPKDREAGWLFTGRGFGHG
                     VGMCQVGSYGMAQRGHSYREILTHYYTGVELGRARITNSGSAVSAPAGQ"
BASE COUNT          466 a         1131 c         1137 g          427 t
ORIGIN      
        1 tcggtgtccg tcgtcgccaa cagcctccgc ctgcgccgag cgcgggtggc ctgatccggg
       61 agactcccat gagcgatccg attctcgaga ccccctccgg ccccacgccc accctcgccg
      121 tctgcggctg tggcacccgc cacctccacc ccgatcgtgg cgagggcgac gagggtgagg
      181 gccgcaaggc cctggccgtg gacccgcagg tcaaggacaa gaacctgaag cgcctgcggc
      241 gcatcgaggg gcaggtccgc ggcctccata agatggtgga ggacgaccgc tactgtgccg
      301 acatcctcac ccagatctcc tccgtccagg aggctctgcg ggcggtgggc cgcgagctga
      361 tgcgcaacca cctcaagcac tgcgccaccg cggccatccg ctccgaggaa ggtcagacag
      421 ccgaggcgat gtacgacgag ctgatcgacc tgatctacaa gaacagccgg tgagtcatcc
      481 ccgcacgatc gcgacgccgc tggaggtccc cagacgggtg gcgccggcgg cgatcagcgc
      541 gcgggcggtg acggcatcgc ggatcccccc tgaggccttg acaccgatcc gcccctccgc
      601 cgtccgggcg aggagctcga catcctcgac ggtggcgccc cccttggcga agccggtgga
      661 ggtcttgacg aagtccgccc ccgcctcgac gaccaggcgg gtgccacgga ccttctcgtc
      721 atcggtcagc aggcacgttt ccaggatcac cttgaggacg agcccctccc gctcggcgcg
      781 gagcgcccgc acccagccgg ccaggtcatc gaggacgcgc cggtcctcgc cgctcttgag
      841 ccaggagatg ttgaggacgg tgtcgagctc gaccgcgccg tccgccaccg cccgccggga
      901 ctcggcgagg cgcgtctccg gatggacgta gccgagaggg aagccgacca ccgtgccggg
      961 gtgcacgccg gtgccggcca atcccgccgc cgccaggggg acgtagaccg gggggataca
     1021 gaccgaggcg aagccgtgct cggccgcctc ggtgatcaga ctccggacct cctcctccat
     1081 ggcgtcgggc cgcagcaggg tgtggtccac atagcgcgcc agggggagcg ccatgccggt
     1141 cactgccccg cgggggcgga tacggcgctg ccgctgttgg tgatgcgggc ccgccccagc
     1201 tcgacccccg tgtagtagtg ggtgaggatc tcgcggtagc tgtgtccccg ctgcgccata
     1261 ccgtacgagc ccacctggca catgccgacg ccgtgaccga agccgcggcc ggtgaagagc
     1321 cagccggcct cccggtcctt gggagccagg cgcttggccg tgaagagcgt gtccggcacg
     1381 tccagagtcc accggatggc gaggccctcg acctccacga cctggccgtt ctccccctgg
     1441 atacggatct tccccacccg gccggactcg ccgcgggaca ggatctccat atcccggaac
     1501 ccgagacccg ggaaacgggt gttgacctgc ttggcgagct gagaatcgct gcggaagcgg
     1561 gtccaggagc tgtacgggct gctgcggtcg aaggcgacgc cgtccaggtc cacctcctgg
     1621 gaggcggcgg cgagccggtc cccgacccag aacagggtca ggtggtcgcc ggtcaccagc
     1681 gccaggtcgg agctggtgac cgcgtccccc tggcgccgga aggtggcgag ctgctccgga
     1741 aagtcatagg tgcgctcctc cttctccttg tcgagcttga ccgccacacg gcggccctga
     1801 tgagagaggt aggagacctc ctcccggcgc accacccgca gcatctcggc gagcgccagg
     1861 agcatcccct cgatctcggt gtcggagagc ggctggtcca gcggaccggt gagcaggccg
     1921 ctcttgacca ggtacgccgc ccgccgcagg tcatcctcgc tccagtcccg gggcgggtcg
     1981 ttgagcaggt actcgacatc ctgtggcgcc aggaacaggc gggcgtccag cgccaggtcg
     2041 aaggcggcgg cgacgaagcg ctggacctcc cgccggtcga gggaggcgag gcgctcccgc
     2101 ggcgcgggca gcccggcgag gagcgccagg tgctccaggc gggcggcgag agcgcgagtg
     2161 gccgagcccc cggcggtcgg cggcagcagg cgctgcgtca agccctccgg gaagtggacg
     2221 ccggccggca gggtaccgtc gagccggtgg atccccccct ccatgcaggg caccccctcc
     2281 aggtacggct ccagcttgag agggaagacg acgggcgcgt cctcggtgtg gccgccgcag
     2341 gtggagctgt agagggcgtc caccagccgt ccttcgaaga gcagcacctg gcccgccgtc
     2401 tcctgaatgg cgcggtcgga aagcgggtgc tccacgtcca tccccttgta gacctggcaa
     2461 cgcggagtgg cgcagatgtc gtacccctcg cgggcgaact caccgaggtt gcgcagggtg
     2521 taggtgcggg cggccaccgc ctgcgccttg agtgcctcga gctggttgta gagctccggc
     2581 cccatctcgc tgggcaccac gccgcgcagg tattgctcca ccggcagctc gttgatcagg
     2641 ttgagcatgc cgcggtcgtt caggtagacc aggatgcggc cgcggtagcg ccggctctgg
     2701 gcgcggatcc cgcgctcgga gggggacgag acggcgagcc agcggccggg gtaggtgcgg
     2761 gggactcccc cctggatcac ccgcagcgcc ggatcggaca cgctggcgcc ggagctgacc
     2821 acgaaggcgc cggtgacgcc gaggagcgtc aggcgccgca gatcctgctc cgccgcctcg
     2881 cgggtggcgt actggccgac ccgcacgcgg tagaggtcga tgccggcgtc gaagctggcc
     2941 tcggcgggct ggccggtctc ccgggccacg cggcgggcga gctcctgcgc ctggcgctcg
     3001 tcgcggagcg ccgccgcctg cacacggtag tacccggcct gggcggcggc cgcggccggc
     3061 tcgatccgca acggtccggt gaccgccagc gcctgccctt cgacggccgc ggtcagcggc
     3121 tcctcacagc agggcagcgt gatggcctcg aggtcgctgg c
//
LOCUS       DASPJI010000820         3900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_400414, whole genome shotgun sequence.
ACCESSION   DASPJI010000820 DASPJI010000000
VERSION     DASPJI010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400414"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..598
                     /locus_tag="VEL74_23175"
     CDS             71..598
                     /locus_tag="VEL74_23175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65503.1"
                     /translation="MDKLNMERLHLLLKGHEELLEEKRAHPEEFLRHNDEFRESFNRV
                     VRSTIVPVLEEIKDVMVGKVESASIFHRLTAAGLKVKLDRWEDYERSLLFFGDEATRM
                     VRITHEGVGFGLLAQKLPLSQITPALVEEEAMKFLRRLFGQEQLRRPLHLEERRRLPG
                     PASGWRGAEAELVRI"
     gene            complement(704..1315)
                     /locus_tag="VEL74_23180"
     CDS             complement(704..1315)
                     /locus_tag="VEL74_23180"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HYG65504.1"
                     /translation="MKTSRILAGFAMGALVVSTASAYVLLSPNRTWDTAPRIIVDSRG
                     ASSITDATRGVSATVAAITAWNGAGAGTMLTAVSGSVSGWRLGDGQPMLNFTDPQRAC
                     KGSCLAATFTGFYNQRADGSYRITDADIVTNTRYAWTSVAEPDGCSSEFYIEGVMVHE
                     VGHLLGLGHSNVSGSTMYPSVASCNNGPATIEADDRNAINALY"
     gene            complement(1338..1898)
                     /locus_tag="VEL74_23185"
     CDS             complement(1338..1898)
                     /locus_tag="VEL74_23185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65505.1"
                     /translation="MKRFFWVGLFALVLTAALPVGASTFVALTPDQLVEQSDAIVQGE
                     VLKINSFWTSDGSVIVSEALVQVTDTIAGDSPTVLRVKTFGGTVGGYTVEAHGFPGFR
                     AGDRVLLFVQNQADGTVEVTGYRQGQYRVAFERGMEKAIPTLESGVNLLTPDGRQAIR
                     PVAKELESFKQQIRERAERARAFVNN"
     gene            complement(2137..2646)
                     /locus_tag="VEL74_23190"
     CDS             complement(2137..2646)
                     /locus_tag="VEL74_23190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65506.1"
                     /translation="MTEDSMSARPYNAFNARFLEMLRKMEDTGTAEVDLAGPWKVVEV
                     ADGWGLFRVWESPGEGHEPLVVCDGHARALRLLAVLEACGREPLYVLGEERGPQGYEL
                     RSAGEPVGRMTRFDPALALLFHGAECLGRSPVALAALLRGSGPQAMQLVGEIVYRDVA
                     AGAEGEGLS"
     gene            2828..3178
                     /locus_tag="VEL74_23195"
     CDS             2828..3178
                     /locus_tag="VEL74_23195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65507.1"
                     /translation="MSDFSNRDIERKLGFSGGYLSRLFGGAIDIKMSHILDVCEIIGL
                     YPHEFFQMAFPPEQGEPSPLMKKILDMTPGSRPIQIPGAPPPPPPPALTPREIQEKIV
                     DALRMVFADLEKPR"
     gene            complement(3632..>3900)
                     /locus_tag="VEL74_23200"
     CDS             complement(3632..>3900)
                     /locus_tag="VEL74_23200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65508.1"
                     /translation="RAHAAMASLDLAVILMHRNASDEACLLADEAFGVFRGLKINDAQ
                     VEALLVLREVLEQRIVTAGFLQSLVDFLRRAEHDPYARYEPRFG"
BASE COUNT          666 a         1328 c         1308 g          598 t
ORIGIN      
        1 cgattagccc atggccggac cgtggcatgg cgcttgctga aaccccacgg gaggaaagga
       61 agggactcgt atggacaaat tgaacatgga gcggctccac ctcctgctga aggggcacga
      121 ggagctgctg gaggaaaaga gggcgcaccc ggaggagttc ctgcgtcaca acgacgagtt
      181 ccgtgagagc ttcaaccggg tggtccggtc gaccatcgtc cccgtcctgg aggagatcaa
      241 ggacgtgatg gtcggcaaag tggagtcggc ctccatcttc caccggctga cggccgcggg
      301 gctcaaggtc aagctcgacc gctgggagga ctacgagcgc tcgctgctgt tcttcggcga
      361 cgaggccacc cgcatggtgc gcatcaccca cgagggcgtg ggcttcgggc tcctcgccca
      421 gaagctcccc ctgtcgcaga tcacccccgc cctggtggag gaggaggcga tgaagttcct
      481 gcgccgcctc ttcggccagg agcagctccg ccgcccgctg cacctcgagg agcgccgccg
      541 tctccccggc ccggcgagcg gctggcgcgg cgcggaagcc gagctcgtcc ggatctgacc
      601 ccgtccgccc cggacccggt catccggacc cgggtcggtc gccaaataga aagggccccg
      661 aaaatcgggg ccctttcctt attgctgcaa agggagcctt ctactagtag agggcgttga
      721 tggcgttgcg gtcgtccgcc tcgatcgtgg ccgggccgtt gttgcaggac gccaccgacg
      781 ggtacatcgt cgagccggag acgttgctgt ggccgaggcc cagcaggtgg ccgacctcgt
      841 gcaccatgac accctcgatg tagaactcgc tggagcagcc gtcgggctcg gcgaccgagg
      901 tccaggcgta gcgggtgttg gtcacgatgt ccgcgtccgt gatgcggtag ctgccatcgg
      961 cacgctggtt gtagaagccg gtgaaggtgg cggccaggca ggagcccttg caggcccgct
     1021 gcgggtcggt gaagttgagc atcggctggc cgtcgcccag gcgccagccc gagaccgagc
     1081 ccgagacggc ggtcagcatg gtgccggcgc cggcgccgtt ccaggcggtg atggcggcga
     1141 cggtggcgct gacgccacgg gtcgcgtccg tgatggagga ggcgccgcgg ctgtcgacga
     1201 tgatccgggg cgcggtgtcc caggtgcggt tggggctgag gagaacgtag gccgaggcgg
     1261 tgctgaccac cagcgcgccc atggcgaagc cggcgagaat gcgcgaagtc ttcatgatct
     1321 gatctctcct ccagtcctta attgttcaca aaggcgcggg cgcgctcggc acgctcccgg
     1381 atctgctgct tgaacgactc gagctccttg gccaccggac ggatcgcctg acggccatcc
     1441 ggggtgagca ggttgacgcc ggactccagc gtcgggatgg ccttctccat gccacgctcg
     1501 aaagcgaccc ggtactgccc ctggcggtag ccggtcacct cgaccgtgcc gtcggcctgg
     1561 ttctgcacga acagcagaac gcggtcaccg gcgcggaagc cggggaagcc atgagcctcc
     1621 accgtgtagc cgcccaccgt gccgccgaag gtcttgaccc gcagcaccgt cggagagtcg
     1681 ccggcgatcg tgtccgtgac ctgcaccagc gcctcgctca cgatcacgct gccgtcggag
     1741 gtccagaacg aattgatctt gagaacctcg ccctggacga tcgcgtccga ctgctccacc
     1801 agctggtccg gagtcagcgc cacgaacgtc gaggcgccta ccggcagagc ggcggtcaga
     1861 accaacgcaa acagaccgac ccaaaagaaa cgcttcatcg aaacctcctt gtccctcatc
     1921 agggttccac ggtatcccgc gccagatcag cgggggaatg agtcagggga tccatgaagg
     1981 gcaacggccg tgccagcctg gagggtcgcg aagagcccac tgaaaacaat aggcttacac
     2041 gggatcgcgg gcgcctcgca acccggggtg gatcaaaaat cgatccaccc cggtggatca
     2101 aggattgacc gcgggtcagt cattaacccc cgccggtcac gagaggccct ccccctccgc
     2161 tcccgccgcc acgtcccgat agacgatctc gccgacgagc tgcatcgcct gcgggcccga
     2221 gccgcgcagc agcgccgcca gggccacggg cgagcggccc aggcactcgg cgccatgaaa
     2281 gaggagcgcc agagccggat cgaaccgcgt catcctgccg accggctcgc cggcgctgcg
     2341 cagctcgtac ccctgagggc cgcgctcctc ccccagcacg tacagcggct cccggccgca
     2401 ggcctccagc accgccagca ggcgcaaggc gcgggcgtgc ccgtcgcaca ccaccagcgg
     2461 ctcgtgccct tcgcccgggc tctcccagac ccggaacagc ccccagccgt ccgccacctc
     2521 caccaccttc cacggccccg ccaggtccac ctccgccgta ccggtgtcct ccatcttccg
     2581 cagcatctcc aggaatcggg cgttgaaagc attgtacggc ctggcactca ttgagtcttc
     2641 ggtcatgcag tcgcctcctt gggttctcca gtccggggac caagatacag atggactgat
     2701 ttaagcgtca acttcttacg cttaattcac tgaagtggag ctccgagtcg ccatggtgta
     2761 gactatgagc actatgtccg acgaacacgt tgcgagactc aactccgtcc tgcgaagcgc
     2821 catccgcttg tccgatttct cgaaccggga catcgagagg aagctcggat tcagcggcgg
     2881 ctatctcagc cgtctgttcg ggggagccat cgacatcaag atgagccaca tcctggacgt
     2941 ctgcgagatc atcggcctct atcctcacga gttcttccag atggccttcc cgccggagca
     3001 aggcgagccg agcccgctga tgaagaagat cctggatatg acgccggggt cccggcccat
     3061 ccagatcccc ggcgcgccac ccccgccccc tccaccggcc ctgacgccgc gcgagatcca
     3121 ggagaagatc gtggacgcgc tccgcatggt cttcgcggac ctcgagaagc cgcggtagcc
     3181 ctcacccctc cctggggctg gcggcccagg ctgaaagaag ggccaccccc ttcgggggct
     3241 tggaaggatg agtccccgga ggggacggcc ctttccttag cctgggccgt cagccccaga
     3301 tttcgagcac cccccttctg gacgccgggc gggggcctcc tccccccctg ggcggtggcg
     3361 tgggagggag cgggggagga ccgaggtggg gggttgtttt tttcgaggcc ggcccggagg
     3421 ccgtaccccg ggaaacgacc gtcctacccg gggtaggaag gccgtacccg gcggcgggag
     3481 gccgtacccg gtcggatgga gcccgcgccc gcggggatca acttcctacc cgcgggtaga
     3541 ggccgctacc cggggggatc gcggccctac cccgggtacg gagcccgtag cccgggggat
     3601 ggcgcctctt tcgcggggga tgccggaagc ctcagccgaa gcgcggctcg tagcgcgcgt
     3661 aggggtcgtg ctcggcccgg cgcaggaagt ccacgaggct ctggaggaat ccggccgtga
     3721 cgatgcgctg ctccaggacc tcccggagca cgagcagcgc ctccacctgg gcgtcattga
     3781 tcttgaggcc ccggaacacc ccgaaggcct cgtcggccag gaggcaggcc tcgtccgagg
     3841 cgttgcggtg catgaggatg acggcgaggt cgagggaggc catggcggcg tgggcccggt
//
LOCUS       DASPJI010000821         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_621120, whole genome shotgun sequence.
ACCESSION   DASPJI010000821 DASPJI010000000
VERSION     DASPJI010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621120"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2280
                     /locus_tag="VEL74_23205"
     CDS             <1..>2280
                     /locus_tag="VEL74_23205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65509.1"
                     /translation="LTGDEAWAVHPATNPGREGLGAGNLAHVLFTSGSTGRPKGVAVS
                     HRNVVRLVRESRFADLGPDQVFLQFAPLAFDASTLEIWGSLINGGRLALFPPGPASVE
                     ELAGVVAEQGVTTLWLTAGLFHRMVEARPAGLESLRQLLAGGDVLSPAHVRQALEMLP
                     GCTLINGYGPTENTTFTCCHAMTDPGAVEAPVPIGRPISNTRVYVLDGHLLPVPPGTY
                     GELYAAGDGVARGYVRRPELTAERFLPDPFAASPGGRLYRTGDLARFRADGTVEFLGR
                     NDQQVKIRGFRIEPGEVEAVLAEHPAVSAAAVVVRRDGQAGGEARLAAYFIPREPVEA
                     GELVRHLERRLPSYMVPADFVSLAALPLTANGKVDRRALPEPPRPVSSTVTNRRWPRD
                     PQEVLLAALFAEVLRLDRVGIDDDFFALGGHSLLATQLVSRVRQSLGVELPLRALFEE
                     PTVADLAAVIARTAREELPPVEPAQRDRPLPLSFAQERLWFLDRFEPGSAAYNMPAAV
                     RLTGRLGVPAFAAALGEIARRHEALRTTFGTGADGAPVQEIAPWEPLPLPVLDLSGLP
                     SAVREEEARRLAVEEARRPFDLARGPVLRGGLVRLGEVEHLAQLTLHHIAGDGWSMGI
                     LIRELTVLYGALLEGCPSPLRELPLQYADFAVWQRGWLAGERLAAEIAHWRERLAGAP
                     PALDLPSDRPRPAVRTSQGASLPFALPADLSRELRALSRDRGVTLFMTLLAGLQTLFA
                     RYTGQIDVSVGTPIAGRHHL"
BASE COUNT          256 a          862 c          819 g          343 t
ORIGIN      
        1 ctcaccggtg acgaggcgtg ggcagtccat ccggcgacga atcccggccg cgagggactc
       61 ggggcgggga acctggcgca cgtgctcttc acctcgggct ccaccggccg tcccaagggg
      121 gtcgcagtct cccatcgcaa cgtcgtccgc ctggtgcgcg agagccgctt cgccgacctc
      181 ggcccggacc aggtcttcct gcagttcgcg cccctcgcct tcgatgcctc gaccctggag
      241 atctggggat cgctgatcaa cggcggccgg ctggcgctct tcccgccggg gcctgcctcc
      301 gtcgaggagc tggccggggt ggtggccgag cagggggtga ccacgctctg gctgaccgcc
      361 ggcctcttcc accggatggt ggaggcccgg cccgccggcc tggaatcgct ccgccagctc
      421 ctcgccggtg gcgacgtcct ctctccggcg cacgtgcggc aggctctgga gatgctcccc
      481 ggctgcacgt tgatcaacgg ctacggtccg accgagaaca ccacgttcac ctgctgccac
      541 gcgatgacgg accccggcgc ggtggaggcg ccggtaccca tcggccggcc gatttccaac
      601 acccgggtct acgtgctcga cgggcacctg ctgccggtgc cgccggggac ctacggcgag
      661 ctctacgcgg cgggggacgg cgtcgcccgg ggctacgtcc gccggccgga actgacggcg
      721 gagcgctttc tgcccgaccc cttcgccgcc tctcccggcg gccggctcta ccgcacgggg
      781 gacctagccc gcttccgggc ggacgggacg gtggagttcc tgggccgtaa cgaccagcag
      841 gtgaagatcc gcggcttccg catcgagccc ggcgaggtgg aggcggttct cgccgagcac
      901 cccgcggtgt ctgccgccgc cgtggtggtc cggcgcgacg gccaagcggg aggtgaggcc
      961 cgcctggccg cctacttcat cccccgggag ccggtggagg ccggagagct cgtccgccat
     1021 ctcgagcggc ggctcccgtc ctacatggtg ccggcggatt tcgtgtccct ggcggccctc
     1081 ccgctcaccg ccaacggcaa ggtggaccgc cgggcgctgc cggagccgcc gcggccggtg
     1141 tcaagcacgg tcacgaaccg ccggtggccg cgggacccgc aggaggtgct tctggcggcc
     1201 ctcttcgccg aggtgctgcg cctcgaccgg gtgggcatcg acgacgactt tttcgccctg
     1261 ggcggccact ccctcctcgc tacccagctc gtgtcgcggg tgcgccaatc gctcggggtg
     1321 gagctgccgc tccgtgccct gttcgaggag ccgaccgtgg cggatttggc cgcggtcatc
     1381 gcccgcaccg cccgggagga actgccgccc gtcgagccgg cacagcggga ccggccgctc
     1441 ccgctctcgt tcgctcagga gcggctctgg ttcctcgacc gcttcgagcc cgggagcgcc
     1501 gcctacaaca tgccggccgc cgtgcgcctg accggccggc tcggcgtgcc ggcgttcgcc
     1561 gccgccctgg gggagatcgc ccgccgccac gaggcgctac gcaccacctt cggcacgggg
     1621 gccgatggcg cgccagtgca ggagatcgcg ccctgggagc cgctgcctct gccggtgctg
     1681 gacctctcag gccttccgtc tgccgtgcgc gaggaggagg cgcggcggct ggccgtggag
     1741 gaggcgcggc gaccgttcga cttggcccgc ggtccggtgc tgcgcggcgg cctggtgcgc
     1801 ctgggtgaag tggagcacct ggcgcagctc accctgcatc acatcgcggg cgacggctgg
     1861 tccatgggca tcctgatccg cgagctgacc gtgctctatg gcgccctgct ggagggatgc
     1921 ccgtcgccgc tacgcgagct gcctctgcaa tacgcggact tcgccgtctg gcagcgcggg
     1981 tggctggcgg gggagcggct ggcggccgag atcgcgcact ggcgggagcg gctcgccggc
     2041 gcgccgccag cgctcgacct gccctcggac cggccgcggc ccgccgtccg cacgtcgcag
     2101 ggcgcgagcc tgccgttcgc actgccggcg gacctctccc gcgagctgcg ggcgctgtcc
     2161 cgcgaccgcg gcgtcaccct cttcatgact ctgttggccg gtctccagac tctcttcgcc
     2221 cgctacaccg gccagatcga cgtgagcgtc ggcactccca ttgccggccg ccatcacctg
//
LOCUS       DASPJI010000822        12608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_796483, whole genome shotgun sequence.
ACCESSION   DASPJI010000822 DASPJI010000000
VERSION     DASPJI010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 12608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12608)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12608
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796483"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..8095
                     /locus_tag="VEL74_23210"
     CDS             17..8095
                     /locus_tag="VEL74_23210"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65510.1"
                     /translation="MTPNGKVDRKALPAPQSAARERAGAETRLTEMQELLAGIWSEVL
                     GRGRVGAGDNFFDLGGHSLLATRVASRVRTVLGVELPLRAIFEQPTVAGLAERVQAAQ
                     GIPQPPPIVPVSRDTPEGGLPLSFAQQRFWLLDQLKQDSAAYNIPLAVRMTGALQVGL
                     LERVFAEMVRRHETLRTTFASREGSPIQVIAAPRVELPVLDLSHLPDPEERALGLSYE
                     EAWRPFDLERGPLLRLVLLRLGERDHVLLLTMHHIISDAWSMGVLLREIGALLAASPL
                     PKLPVQYADFAVWQRSWLQGEVLEAQLAYWRHQFAGAPQVLELPTDRPRPAAQTFRGA
                     SQKICLPPELSAAVHGLCRREGVTPFMALLAAWAVLLGRHANQEDVLVGTSVAGRNRR
                     EVEDLIGLFVNTLALRVHLTESPSGAPGFGELLGRVRRAALDGYTHQDVPFERLVEEL
                     APERSQAHSPLFQVMLVLQNAPGEGLSLPGLTLTPLALDGEVAKFDLTLALEERRDRV
                     FEGALEYNTDLFDGSTAVRMLERWQLLLDRAVADPARPVAELPLLTEAERFQLLSTWN
                     DTAAAPLAEPWLHVRFQAVAARYLGEVALIQAERRLTFEDVAHRAERLAAGLADLGVG
                     PEVVVGLRFERSPELVIAALGVLMAGGAYLPLDPAHPEERQATLLADAGAALVLSRET
                     WPDAAARALPAVDPEGLAYVLYTSGSTGRPKGVMVQHRALARYLDWAIEAYAVEAGQG
                     APVHSSLAFDLTVTGLWVPLLAGRPVTLLPEEHGVEALAATVRPVADFSLVKLTPAHL
                     DLLAQQVAPESVAGWTRALVVGGEALRQESLAFWRAHAPATRIFNEYGPTETVVGCSV
                     YEVPVSAGSLPIGRPIAGARLYVLDRGLHPSPVGVIGELWIGGEGVTRGYRRDPWQTA
                     ERFFPDAFSTAPGARLYRSGDLARWRANGVLEYLGRTDHQLKVRGFRIEPGEIESVLR
                     RHPAVRDVAVLARPGALGDASLAAFVAADPAALTGAELRDLLARHLPDYMVPAAMVVV
                     DDLPLTSNGKVDREALLRLEPVPQEVAASPAAPRTPEEELMAGLFAQVLGIEQVGLDD
                     NFFDLGGHSLLAMQLIARVREIWQAKVSLVAFFKIPTVASLLARLRSPSGAARELPPP
                     FERGPAGEDAPLSFAQMRLWIVDQIEPDSPAYNIPFALRLRGRLSREALDAAIGEVVR
                     RHEILRTTFPAVLGDPVQRISPPCRHLSPLLDLSALRETREDQVLALAREEVLRPFDL
                     AAGPLFRSTLLRLGDADHVLLFTLHHIIGDGWSTGVLVREVGEFYRALLAGRPSSLPE
                     LPIQYADYSRWQRRWLQGEVLEEQLEYWRRQLAPPLPVLDLPTDRPRPAAPSGHGDFC
                     ELPLTPELSDALIGLGRQQGTTLFMTLLAGFYALLQRYTSQDDLVIGTDIANRGNLET
                     QALIGFFVNQLVLRVGIGPAASFEELLGQVRTMALEAYGHQDLPFERLVKELQPERDL
                     ARQALFQVTFTLQNDSPSKLDLEGLTLEPLQLGSATAKLDLRVEARQIGRRLILGAEY
                     SLDLFDDVRIERLLLHFAALLQEVAARPQGRLSEFDLLSAAERSCLMLEIGDGGEAPV
                     SPGFMPELFFRRAAEHPDVVALAHGGEQWTYGALAAWTSSLARVLRRSGVGCEARVGV
                     FGERGPGMLAAMLAILEAGGAFVPLEPQLPDARLASILATAQPRCIVASRRFDTRAEL
                     LAGPAAVLLWDDDGRAEGSPRREPPAPIDPRQLACVFYTSGSTGMPKGAMVEHQGMLN
                     HLLAKVELLALSPGSVVVQNASHGFDISVWQFLAALLAGGRVVVYDEETALDPIALLR
                     ALARDGATVLETVPTLLEPLLTMSSQLAAEGEEIALPALSCLISNAETLPVPLARRWF
                     EQYGEVALFNTYGATECSDDTTHWRMREAPGEGCVRVAVGSPIRGFRIHVVDRWGRLI
                     PRGCVGEIAMAGVGVGRGYLGDAEKTARTFMPDPFSGLIGERMYRTGDLGRWTEQGEL
                     EFLGRRDHQVKVRGHRVELGEIEAVLGRHPQVREVAVLAREDGHGPARLVAYVIPRTS
                     EPEEGTGEPELRLRDFVAGALPAWMVPSAFVTLPEFPLTPNGKLDRRALPAPDARPEL
                     AEDYTAPQGAVEKTLAEIWAQVIGIDRVGLHDNFFELGGDSILAIQIAALAHRASLGV
                     TPRDLFLHQTVAELAVVARSQEIVRVTQELVSGPVPLTPIQHWFFEQGLAAPWHFNQT
                     VFLQIQRRLDPAALHRVVAQLLRHHDALRLRFWRQGDEWRQLHAAPDESVPFAHIDLT
                     MVPESLRASVIEAAAAAAQTSLDLAAGPLLRYVYFDLGPHLPPRLLLIVHHLVVDGVS
                     WRVLLEDLETGYRQAERGEAIELPAKTSSFKAWAERLDEHVRAGGLEEEAAYWLATER
                     ALAAPLPVSFPEGANTVSSARTVSRTLGSEETTLLLQEVPKAYSTQIDDVLMTALALA
                     CRSWLGGGAIQVVLESHGRIGLFADLNLSRTVGWFTSEAPVLLDVNGAATPGDALKSV
                     KEQLRAVPNEGVGYGALRYLGPQWIRDRLRELPPAQMIFNYLGQLDRVLPADSLFLLA
                     EEASGPTLSPHARREHVLELEAGIAGGRMSLSCTYSENLHARATIERLADSLLAELRS
                     VIAHCADPDVAGYTPSDFPQMQISQGELDDLLSHLEEF"
     gene            8095..>12608
                     /locus_tag="VEL74_23215"
     CDS             8095..>12608
                     /locus_tag="VEL74_23215"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65511.1"
                     /translation="MSRARAVENLYPLSPMQKGLLFHTIGSPDSGLYITQSVYELRGE
                     VERQALARALQSVLDRHSVLRTAFHWERRDEPFQVVYKEARVPLQELDWREVLPAELE
                     RRQQDFLRQDRQHGFDIARPPLLRVALIRLTESSWRLVWSHHHLLLDGWSLPLLFNEF
                     AAFYEAFRQQRELQLPRPRPFANYIAWLQGQDLSQAETFWRHLFQGFSPSADGLEGRV
                     LPSAPEEDGERAGEAAVERGHLFGTEETERLAAFCRQHQLTLNTLILGAWSLLLGRVS
                     GSDDVVFGVTVSGRPPELEGVETMVGVFINTLPLRVVMPGQQQVLPWLTRLQLQQVEL
                     RRFEHTPLVDIQRWSRIPANQPLFDKFVVFENYPGDRRSSAGQVSLQSEQVSTVITTN
                     YPLGLIVGPAGRLLLKLVYHAARYDDVAILRLLEHLRALLEGLVSRPLQLLMDVPWLT
                     PAEAHQLRTEWTEMHPASLPVSSIPAAFEQWAERTPQATALVCAGEERTYGDLNRAAN
                     RLAWHLLRHGAGAGVRVGICVERSIAMIVGLLGILKTGAAYVPLDPANPAERLEYMLA
                     DSGARLLVVRGAWPGGEPAASVTLVDLDGLNLDREDNEDGEDPKIEISSGSLAYVIYT
                     SGSTGRPKGVLMTQANVLRLFASTRERFGYGSADVWTLFHSYAFDFSVWEIWGALLHG
                     GRLVVVPFAVSRSPESFHALLRQQGVTILSQTPSAFYQLIWVESILREREGSAPALPR
                     LRLVVFGGEALDFAQLAPWIEHHGDRQPELNNMYGITETTVHCMQRRIHRGDLPTGRS
                     VIGRPHPDMSIHVVTPGSALVPLPVPIGVPGEIWVGGGGLAMGYLGRPALTAERFIPD
                     PFGTEPGGRLYRSGDLARHLDSGDIQYLGRIDFQVQIRGFRVELGEIETALHLHTAVL
                     QSVVLAHGGGRAAGTGTRLSAYIVLRTGGPLTAADLRAYLASRLPEHMVPSRFFFLES
                     LPLTNSGKVDRQALALLEGEDGDGEEAVHQEPRTPVEELLAALWSEVLETRVGIHDDF
                     FESGGHSLLAMRLISRVRASFQVELPLRALMEHPTVAGLARELEDAKRREMGVSAPPI
                     ERTALDEPPPLSFAQQRLWFIQQMEPESSAYNLATPWRVRGLLEVDVLARSLREIVRR
                     HEVLRTTFESRQGGTVQIVHTVDLRFLALIDLSALPAPRRWAESSRLTRAEARRPFDM
                     ERGPLLRTALLRLEPEEHVVLFTMHHVITDGWSADLLIKELKALYGAFLAGRPSPLDE
                     LPVQYADFAVWQRSWLQGEVLDSQLAYWRRRLGDDPEPLDLPADRPRPLVRSDRGGVF
                     ELVLDEAAALAGLAQRERVTLFMTLLPAFQICLHRLTQQRTLNVGTPVSGRHHRQLED
                     LIGFFANILVLRSDTADGLGFRQHLLQAKETTLEAYAHQDLPFEKLVDELKIERDLRR
                     SPLFQVVFTLQNAGGEELRFPGLELSPWESGSEVSLFDLTLTVMQRPGGLVGSLAFSR
                     DLFDTATIARL"
BASE COUNT         1720 a         4311 c         4475 g         2102 t
ORIGIN      
        1 cctggaagcc ctgccgttga ctcccaacgg caaggtggac cgtaaggcgt tgccggcgcc
       61 gcagtcggcg gcgcgtgagc gggccggcgc ggagacccgg ctgaccgaga tgcaggagct
      121 gttggccggc atctggtccg aggtcctggg ccgcggtcgc gtcggcgccg gggacaattt
      181 cttcgacctc ggtgggcact cgctgttggc cacgcgggtg gcctcgcggg tgcggacggt
      241 cttgggcgtc gagctgccgt tgcgggcgat cttcgaacag ccgacggtgg cggggctggc
      301 ggagcgcgtt caggcggcac aagggatccc gcaacctcct ccgatcgtgc ccgtgtcgcg
      361 cgacactccg gagggcggcc tgccactctc cttcgcccag cagcggttct ggctcctgga
      421 ccagctcaag caggacagcg cggcgtacaa catccccctg gcggtgcgga tgaccggtgc
      481 gcttcaggtc ggtctgctgg aacgggtctt cgccgagatg gtgcgccggc acgagacgct
      541 gcgcaccacc ttcgcctctc gcgaggggag ccccatccag gtgatcgccg caccccgggt
      601 cgagctgccg gtgctggacc tgtcgcatct accggatccg gaggagcggg cgctcggtct
      661 ctcctacgaa gaggcgtggc ggccgttcga cctggagcgc ggaccgctgc tgcgcctggt
      721 gctgttgcgg ctgggcgagc gggaccacgt cctgctgctg acgatgcacc acatcatctc
      781 ggacgcctgg tcgatgggcg tgctgctgcg cgaaatcggg gccctgctcg cagcttcccc
      841 gctccccaag ttgccggtgc agtacgccga cttcgccgtc tggcagcgga gctggctgca
      901 aggcgaggtg ctggaggcgc agctcgccta ttggcggcac cagttcgcag gcgcgccgca
      961 ggtgctggag ctgccgacgg accggccgcg gccggccgca cagaccttcc ggggagcgtc
     1021 acaaaaaatc tgcttgccgc cggagctgtc ggcggcggtg cacgggctct gccggcgcga
     1081 gggcgtgacc cccttcatgg ccctgcttgc cgcctgggcc gtcctgctgg gccgtcacgc
     1141 caaccaggag gatgtgctgg tgggcacgtc ggtcgccggc cgcaaccgcc gcgaggtcga
     1201 agacctgatc ggccttttcg tcaacaccct ggcgctgcgg gtccatctga cggagagccc
     1261 gtccggcgct ccgggcttcg gcgagctgct gggccgggta cggcgtgcag ccctcgacgg
     1321 ctacacccat caggacgttc ctttcgagcg gctggtggag gagctggcgc ccgagcgcag
     1381 ccaggcccac tcgccgctgt tccaggtcat gctcgtgctc cagaacgcgc ccggagaggg
     1441 cctgtcgctg ccggggctta ccctcacgcc gctcgccctg gacggagagg tggcgaagtt
     1501 cgacctcacg ttggccctgg aggagcggcg ggaccgggtc ttcgaaggcg cgctggagta
     1561 caacaccgac ctgttcgacg gcagcacggc ggtccggatg ctggagcggt ggcagctcct
     1621 gctcgacagg gcggtggccg atccggcgcg cccggtcgcg gagctgccgc tactgactga
     1681 ggcggagcgg ttccagttgc tgtccacctg gaacgatacg gcggcagcgc ctctcgccga
     1741 gccctggctc cacgtcaggt tccaggcggt ggccgcccgg tatctgggcg aggtggcgct
     1801 gattcaggca gagcggaggc tcaccttcga ggatgtggcc catcgggcgg agcgtctcgc
     1861 cgccggtctc gcggacctgg gcgtcggtcc ggaggtcgtg gtggggctgc ggttcgagcg
     1921 ctcgccggag ctggtgatcg cggctctggg cgtgctgatg gccggcgggg cgtacctgcc
     1981 gctcgaccct gcgcatcctg aggagcgcca ggctacgctg ctcgccgatg ccggcgccgc
     2041 gctcgtcctc tcccgcgaga cctggcccga tgctgcggct cgagctctgc cggcggtcga
     2101 ccccgaaggc ttggcctacg tgctctacac ctcggggtcg accggccgtc ccaagggggt
     2161 catggttcag caccgggcct tggcgcgtta cctcgactgg gcgatcgagg cctatgcggt
     2221 agaggccggc cagggagcgc cggtccactc ctcgctggcg ttcgacctga cggtgaccgg
     2281 tctgtgggtg ccgttgctgg ccggccgccc ggtgacgctg ctgcccgagg agcacggcgt
     2341 cgaggcgctc gccgcgaccg tccgccccgt agcggacttc agcctggtca agctgacgcc
     2401 ggcccatctg gatctccttg cccaacaggt cgctccggag tcggtggcgg gctggacgcg
     2461 ggcgctggta gtgggcggtg aggcgttgcg gcaggagagc ctcgccttct ggcgcgcgca
     2521 cgctcccgcg acgcggatct tcaacgagta cggccccacc gagacggtgg tgggctgctc
     2581 ggtgtacgag gtcccggtgt ccgccggttc gttgccgatc ggccggccga tcgccggagc
     2641 gcgcctgtac gtgctggacc gtggcctcca tccgtcgccg gtgggggtga tcggcgagtt
     2701 gtggatcggc ggcgaaggcg ttacccgtgg ctaccggcga gacccgtggc agaccgcgga
     2761 gcgcttcttc cccgatgcgt tctccaccgc tccgggggcg cggctctacc ggagcggcga
     2821 cctggcgcgc tggcgcgcga acggcgttct cgagtacctc ggccgtaccg accaccagct
     2881 caaggtccgc ggtttccgga tcgagccggg cgagatcgag agcgtgctgc gccgccaccc
     2941 tgccgtgcgc gacgtggcgg tgctggcgcg ccccggcgct ctcggcgatg cgagcctcgc
     3001 cgccttcgtc gcggccgatc ccgcggctct gacgggggcg gagctgcgcg acctcctcgc
     3061 gcgccacctg ccggactaca tggtgccggc cgcgatggta gtggtggacg atctacccct
     3121 gacgtcgaac ggcaaggtcg accgcgaggc gctcctccgg ctcgagcccg taccgcagga
     3181 ggtggccgcg agcccggcgg caccgcgcac ccccgaagag gagctgatgg ccggcctctt
     3241 cgctcaagtg ctcgggattg agcaggtcgg cctcgacgac aacttcttcg atcttggcgg
     3301 tcactcgctg ctggcgatgc agctgatcgc gcgagtgcgc gagatctggc aggcgaaggt
     3361 gtcgctggtc gctttcttca agatacccac cgtcgccagc ttgctggccc gtctccgctc
     3421 gccctcggga gccgcgaggg agctcccgcc gccgttcgag cgcgggccgg cgggcgagga
     3481 cgccccgctg tcgtttgccc agatgcggct gtggattgtc gatcagatcg agccggacag
     3541 cccggcctac aacatcccat tcgccctccg gctgcgtggg cggctgtcac gggaggcgct
     3601 ggatgctgcg atcggcgagg tggtgcggcg gcacgagatc ctgcgcacca ccttcccggc
     3661 cgtgctgggc gaccccgtgc agcggatatc gccgccttgc cggcatctct cgccactcct
     3721 cgatctctcc gcgctccggg agaccaggga ggatcaggtg ctcgcgctgg ccagggagga
     3781 ggtcctccgg ccgttcgatc tcgcggccgg accgctcttt cgctcgacgc tgctgcggct
     3841 gggcgacgcc gatcacgtgc tgctcttcac gctccaccac atcatcggcg acggctggtc
     3901 gaccggtgtg ctggtccgtg aggtcggcga gttctaccgt gccctgctgg ccggccgccc
     3961 ttcctctctg cccgagctgc cgatccagta cgcggactac tcgcgctggc agcggcgctg
     4021 gctgcaggga gaggtactcg aggagcagct cgagtattgg cgccggcaac tggcgccgcc
     4081 gttgccggtc ctggacctcc cgaccgaccg gccgcgtcca gcggcgccca gcggccacgg
     4141 tgacttttgt gagctcccgc tgacgccgga gctgtccgac gccttgatcg gcctcggccg
     4201 ccagcagggc accacgctct tcatgaccct gctggccggc ttctacgccc tgctccagcg
     4261 ttacaccagc caggacgacc tggtgatcgg taccgacatc gcgaaccgcg gcaacctgga
     4321 gactcaggcg ctgatcggct tcttcgtcaa tcagctcgtg ctgcgggtcg ggatcggccc
     4381 ggcagcgagc ttcgaggagc tgctcgggca ggtgcggacg atggcgctcg aagcctatgg
     4441 ccaccaggat ctccccttcg agaggctggt caaagaactg cagccggagc gcgatctcgc
     4501 ccgccaagca ctcttccagg tcactttcac gctccagaac gattcgccgt cgaagctcga
     4561 tctggagggc ctgactctgg agcccttgca actcggcagc gcgacggcga agctcgatct
     4621 gcgtgtcgag gcaaggcaga tagggaggcg cctcatcctg ggcgccgaat acagcctcga
     4681 cctcttcgat gacgtgagga tcgagcgcct gctgctgcat ttcgcggccc tgctgcagga
     4741 ggtcgccgcc cgcccccagg ggcgactctc cgaattcgat ctgctgtcgg cggcggagcg
     4801 gagctgtctg atgctggaga tcggcgatgg cggcgaggcg cccgtcagcc ccggtttcat
     4861 gcccgagctc ttcttccgcc gtgccgccga gcatcccgac gtggtggcgc tggcgcacgg
     4921 cggcgagcag tggacgtacg gcgccctggc ggcgtggacc tcctcgctgg cgcgggtgct
     4981 gcgccggagc ggcgtcggat gcgaagcgcg agtcggcgtg ttcggcgagc gcgggccggg
     5041 catgctggcg gcgatgctgg ccatcctcga ggcgggaggc gccttcgtac cgctggagcc
     5101 tcagctgccg gacgcgcggc tcgcctcgat tctcgccacg gcgcagccgc gctgcattgt
     5161 cgcgtcccgg cgcttcgaca cccgggctga gcttctggcc ggtccggccg cggtgctgct
     5221 ctgggatgac gacgggcggg cagaaggctc gccgcgccgc gagccgccgg cgccaattga
     5281 tccccgccaa ctggcttgtg tgttctacac ctcgggctcg accgggatgc cgaagggtgc
     5341 catggtcgag caccagggga tgctcaacca cctgctggcc aaggtcgagt tgctggcgtt
     5401 gagccctggc agcgtcgtgg tgcagaacgc ctcgcatggc ttcgacatct cggtctggca
     5461 gttcctggcg gcgctgctcg cgggcggccg cgtcgtggtc tacgacgagg agacggcgct
     5521 cgacccgatc gctttgctgc gggcgctcgc ccgcgacggc gcgacggttc tggagaccgt
     5581 gccgacgctt ctggagccgc tgctgacgat gtcgtcgcag cttgcggccg agggagaaga
     5641 gatcgctctg cccgctctgt cgtgcctgat ctcgaacgcg gagacgttgc cggtgccgct
     5701 ggcccggcgc tggttcgagc agtatggcga ggtggcgctg ttcaacacct acggcgcgac
     5761 ggagtgctcg gacgacacga cgcactggcg gatgcgggag gctcccgggg agggctgtgt
     5821 gcgggtggcg gtggggagtc cgatccgggg gttccggatc cacgtagtgg accgatgggg
     5881 tcgcctgatt ccgcgcggct gcgtggggga gatcgcgatg gcgggcgtgg gggtggggcg
     5941 cggctatctg ggggatgcgg agaagacggc gaggacgttc atgccggacc cgttctccgg
     6001 gttgataggg gagcggatgt accggactgg agacctgggg cgctggacgg agcaggggga
     6061 gctggagttc ctgggccggc gagaccatca ggtcaaggtg cgggggcatc gcgtcgagct
     6121 gggggagatc gaggcggtgc tgggccgcca tccgcaggtg cgggaggtgg cggtgctggc
     6181 gcgcgaggac ggtcacggcc cggcgcgcct ggtcgcctac gtcatcccgc gtaccagcga
     6241 gccggaagag gggaccggag agccggagct gcgcctgcgc gacttcgtgg ccggggccct
     6301 ccccgcctgg atggtgccgt ccgcgttcgt gacgctgccg gagttcccgc tcacgcccaa
     6361 cggcaagctg gatcgccgcg ctctgccggc tcccgacgcg cggcccgagc tcgccgagga
     6421 ctacacggcg ccgcaagggg cggtcgagaa gacgttggcc gagatctggg cgcaggtcat
     6481 cggcatcgat cgcgtcggcc tccacgacaa cttcttcgag ctcggcggcg actccattct
     6541 ggccattcag atcgccgcgc tggcgcatcg cgccagtctc ggcgtgacgc cgcgggatct
     6601 cttcctgcac cagacagtcg ccgagctggc ggtggtggcg cggagccagg agatcgtccg
     6661 ggtgacacag gagctggtct ccggccccgt gccgctcacc cccatccagc actggttctt
     6721 cgagcagggc ctggcggcgc cgtggcactt caaccagacg gtcttcctgc agattcagcg
     6781 gcggctcgat cccgcggcgc tccaccgggt tgtggcgcag ctcctccggc accacgacgc
     6841 cttgcgcctg cgcttctggc ggcaagggga cgagtggcgg cagctccacg ccgcgccgga
     6901 cgagagcgtg cccttcgcgc atatcgacct cacgatggtg ccggagtcgc tccgcgcctc
     6961 ggtgatcgag gccgcggccg ccgctgcgca gaccagcctc gatctggctg ccggcccgct
     7021 cctgcgctat gtctacttcg atctgggccc ccatctgccc ccccggctgc tgctgatcgt
     7081 ccatcacttg gtcgtggacg gtgtctcctg gcgggtgctg ctggaagacc tcgagaccgg
     7141 ctaccggcag gcggagaggg gggaggcgat cgagctcccg gccaagacca gctccttcaa
     7201 ggcctgggcc gagcggctgg acgagcacgt gcgcgcgggg ggcttagagg aggaggccgc
     7261 ctattggctc gccacggagc gggccctggc cgcgccgctg cctgtgagct tccccgaggg
     7321 tgccaatacc gtctcatccg cccggaccgt ctcccgcacg ctcggctccg aggagaccac
     7381 actgctcctt caggaggtcc cgaaggctta ttccacccag attgacgacg tcctgatgac
     7441 ggcgctggcg ctggcctgcc ggagctggct gggtggcggc gcgatccagg tggtcctcga
     7501 gagccacgga cggatcggtc tgttcgcgga tctgaatctc tcccgcaccg tcggctggtt
     7561 caccagcgag gcgccggtgc tcctcgacgt caacggcgcg gcgacgccgg gcgatgcgct
     7621 caagtcggtc aaggagcagc tccgtgcggt ccccaacgag ggtgtgggct acggagcact
     7681 gcgctatctg ggaccgcagt ggatccgtga ccggctacgc gagctgccgc cggcgcagat
     7741 gatcttcaac tatctcggtc agctcgaccg ggtactcccc gcggactcgc tcttcctgct
     7801 ggccgaggag gcgagcgggc cgacgctcag cccgcacgcc cggcgcgagc acgtcctgga
     7861 gctcgaggcg ggtatcgccg gcgggcgcat gagcttgagc tgcacctaca gcgaaaacct
     7921 ccatgctcgc gccacgatcg agcgactcgc cgacagcttg ctggcagagc tgcgatcggt
     7981 gatcgcccac tgcgcggatc ccgacgtggc cggctatacc ccctccgact tcccacaaat
     8041 gcagatttcc cagggggagc tggacgattt gctctcccac ctggaggagt tttgatgtcc
     8101 cgcgcgagag ccgtcgagaa cctctacccc ctctctccca tgcagaaggg gctgttgttc
     8161 cataccatcg gcagcccgga ctcggggctg tacatcacgc aatcggttta tgagttgcga
     8221 ggagaggtcg agaggcaggc gctcgcgcgg gcgttgcaga gcgtgctcga ccggcactcc
     8281 gtcctccgta ccgcctttca ttgggagcgt cgggacgagc cgttccaggt ggtctataaa
     8341 gaggcgaggg tccccctgca ggagctcgac tggcgggaag tcctgcccgc ggaattggag
     8401 agacggcagc aggacttcct gcggcaggac cgccagcacg gcttcgacat cgcccggccg
     8461 ccgctgctgc gggtcgctct gatccggctc accgagagct cctggcggct ggtctggagc
     8521 catcaccacc tgctgctgga cggttggtcc ctgccgctgc tgttcaacga gttcgccgcc
     8581 ttctacgagg cgttccgcca gcagcgcgag ctacagctgc cgcgcccccg cccgttcgcg
     8641 aactacatcg cttggctgca aggccaggac ctgtcgcagg ccgagacctt ctggcggcac
     8701 ctgttccaag gcttctcgcc gtctgccgac ggactggagg gccgtgtcct gccgtccgcc
     8761 cccgaggagg acggcgagcg agccggcgaa gcggccgtgg agcgggggca cctgttcggg
     8821 acagaagaga cggagcgcct ggcggccttc tgccgtcagc accagctcac gctgaacacg
     8881 ctgatcctgg gggcctggtc gctgttgctc ggccgcgtga gcggcagcga cgacgtggtc
     8941 ttcggcgtca ccgtctccgg gcgcccgcca gagctcgaag gggtggagac catggtgggg
     9001 gtcttcatca acactctgcc gctgcgggtg gtgatgcccg gccagcagca ggttctcccc
     9061 tggttgaccc ggcttcagct ccagcaggtg gagctgcgac ggttcgagca cacccccctg
     9121 gtcgacatcc aacgctggag ccgcatcccc gccaaccagc cgctgttcga caagttcgtc
     9181 gtgttcgaga actaccccgg agaccggcgg tcgtccgccg gtcaggtctc tctgcaaagc
     9241 gagcaagtga gcacggtcat cacgaccaac tacccgctcg ggctgatcgt gggaccggcc
     9301 ggcaggctgc tgctgaagct cgtctaccac gccgcccgct acgatgacgt ggcaatcctg
     9361 cggctgctcg agcacctgcg agccctgctc gaaggactgg tctcccgccc cctgcagctc
     9421 ctgatggatg tgccgtggct cacgccggcg gaggcgcatc agctccgcac cgagtggacc
     9481 gaaatgcacc cggcgtcgct gccggtctct tcgatccccg ccgctttcga gcagtgggcc
     9541 gaacggacgc cgcaggcgac cgctctggtc tgcgccggcg aggagaggac ctacggcgac
     9601 ctgaaccggg cggccaaccg actcgcctgg catctgctgc ggcacggcgc cggcgcgggg
     9661 gtgcgggtcg gcatctgcgt cgagcgttcg atcgcgatga tcgtgggctt gctgggaatc
     9721 ctcaagaccg gcgccgccta cgtgccgctc gaccctgcga atccggccga gcggctggag
     9781 tacatgctgg cggacagcgg cgcgcggttg ctcgtggtgc gcggcgcctg gcccggcggg
     9841 gagccggccg ccagcgtcac gctcgtcgac ctggacggat tgaatctcga ccgcgaggac
     9901 aacgaggacg gcgaggatcc gaagatcgag atctcctccg ggtccctggc ctacgtgatc
     9961 tacacgtcgg gttcgaccgg ccggccgaaa ggggtcctca tgacccaggc gaacgtgctc
    10021 cgcctcttcg cctcgacccg ggagcgcttc gggtacggca gcgccgacgt ctggaccctc
    10081 ttccattcgt atgccttcga tttttcggtc tgggagatct ggggcgctct gctccacggc
    10141 ggccggctgg tcgtggtccc cttcgccgtc agccgttcgc cggagagctt ccacgctctc
    10201 ctgcgccagc aaggcgtgac gatcctcagc cagaccccct cggcctttta ccagctcatc
    10261 tgggtcgagt cgatcctgcg ggagcgggag gggagcgcac cggccctgcc gcgcctgcgc
    10321 ctggtggtct tcggcggcga ggccctcgat ttcgcacagc tcgcgccgtg gatcgagcac
    10381 cacggcgacc ggcagcccga gctgaacaac atgtacggga tcacggagac gaccgtccac
    10441 tgcatgcagc gccggattca tcggggggat ctgcccaccg gccgtagcgt catcggccgg
    10501 ccgcacccgg acatgtcgat ccatgtggta acccccggct ccgcgctggt gccgctcccg
    10561 gtgccgatcg gtgtaccggg agagatctgg gtcggcggcg gcggcttggc catgggatat
    10621 ctcggccgtc ccgcgctgac ggccgaacgc ttcattccgg accccttcgg caccgagccc
    10681 ggggggcggc tctaccggtc gggggacctc gcgcgccatc tcgacagcgg tgacatccag
    10741 tatctgggac gcatcgactt ccaggtccag atccgtggct tccgcgtcga gctgggggag
    10801 atcgagacgg cgctgcacct tcacactgcg gttctccaga gcgtggtcct ggcccatggg
    10861 ggcgggagag cagcggggac cgggacgagg ttgtccgcct acatcgtcct gcggaccggc
    10921 gggcctctca ccgccgccga tctgcgggcg tatctcgcga gccggctacc ggagcacatg
    10981 gtgccgtccc gcttcttctt cctggagtcg ctgcccctga cgaacagcgg caaggtggac
    11041 cggcaggccc tcgcgctcct ggagggggaa gacggggacg gcgaggaggc cgtccaccag
    11101 gagccccgca cgccggtcga ggagcttctc gccgcgctct ggagcgaggt gctggaaacc
    11161 cgggtcggga tccacgatga cttcttcgag tccggcgggc attcgctgct cgccatgcga
    11221 ctgatctccc gggtccgcgc cagcttccag gtggaactgc ctctccgcgc gctcatggag
    11281 catccgactg tggcggggct ggcccgggag ctcgaggacg cgaaacgccg ggagatggga
    11341 gtgagcgcgc ctcccatcga gcggacggcg ctggacgagc cgccgccgct gtcgttcgcc
    11401 cagcagcgcc tctggttcat tcagcagatg gagccggaga gcagcgccta caacctcgcg
    11461 acgccctggc gggtccgggg cctcctcgag gtcgacgtgc tagcccgcag cctgcgggag
    11521 atcgtgcgcc gtcacgaggt gctgcgcacc accttcgaga gccgccaggg agggaccgtg
    11581 cagatcgtcc acacggtgga cctcaggttc ctggcgctga tcgacctctc cgccttgccg
    11641 gcgccgcggc ggtgggccga gagctcccgc ctgacccggg ccgaggcgcg ccggccgttc
    11701 gacatggagc ggggaccgct gctgcggacc gctctcctgc ggctcgaacc ggaggagcat
    11761 gtcgtgctgt tcaccatgca ccacgtgatc accgacggtt ggtcggcgga tctgctgatc
    11821 aaggagctga aggccctcta tggcgccttc ctcgccggcc ggccgtcgcc gctcgacgag
    11881 ttgccggtgc agtacgccga cttcgccgtc tggcagcgtt cgtggctgca aggagaagtt
    11941 ctcgactcgc aactcgcgta ctggcgccgg cgcctcggcg acgatcccga gccgctcgat
    12001 ctgccggccg atcgcccgcg gccgctggtg cgcagtgatc gtggaggggt tttcgagctc
    12061 gtcctcgacg aggcggcggc tctcgccgga ttggcgcagc gcgagcgtgt gaccctgttc
    12121 atgaccttgc tcccggcgtt tcagatctgc ctccatcgcc tcacgcagca gcgcaccctc
    12181 aacgtcggga ccccggtatc ggggagacat caccggcagc tggaagatct gatcggattc
    12241 ttcgccaaca tactggtgct gcgctccgac acggcggacg ggctcggctt ccgccagcac
    12301 ctgctgcagg cgaaggagac caccctcgag gcgtatgccc atcaggacct gccgttcgag
    12361 aagctggtcg acgagctgaa aatcgagcgc gatctacgcc gctcgccgct cttccaggtc
    12421 gtcttcacgc tgcagaacgc cggcggggag gagctgcggt tccccggcct cgagctctct
    12481 ccctgggagt cgggctccga agtctctctc ttcgatctga ccttgacggt gatgcagcgt
    12541 ccgggaggcc tggtcggctc gctcgcgttc agccgcgatc tgttcgacac cgcgaccatc
    12601 gccaggct
//
LOCUS       DASPJI010000823         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_730883, whole genome shotgun sequence.
ACCESSION   DASPJI010000823 DASPJI010000000
VERSION     DASPJI010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730883"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2071
                     /locus_tag="VEL74_23220"
     CDS             <1..>2071
                     /locus_tag="VEL74_23220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65512.1"
                     /translation="ELVTERGSLEHSPLFQVMLAVQNTPTVAEGVVAPGLELRPVAAE
                     SATAKFELSLGFALLGEELTGGLEYNTDLFDASTAERLARHFARLLEVAMAAPEMAWP
                     ELPLLDRSEEQQVLLEWNDTEAAYPGSGRCLHELVFEQAERAPEAVAVVAGEEGEEHL
                     THAGLRRRAGWLAHRLRGLGVGREVPVALCAERSLEMVVGLLGILEAGGAYVPLDPDY
                     PADRLAFMVEDAAAPLLLAQRHLVDRLPELRGVRIVFLDELARPEAGAPPAVPDGAGP
                     DHLLYVIYTSGSTGRPKGVMVRHRGVVNRLLWAAHAYPVTAADRVLQKAPFSFDFSVW
                     ECFGTLLSGARLVLARPGGQRDGAYLARTVVEQGITLLHFVPAMLQAFLEEEGLDGCR
                     SLRFVFSGGDALASETVERFQARMPAGVLLRNQYGPTEISIDVTEWICREERTGRTPP
                     IGRPLGNGRVYVTGPNGELVPVGVAGELLLGGVGVARGYLGRPDLTAERFVPFPWQDE
                     AGARLYRTGDRVRLLADGNLEFLGRVDHQVKIRGYRIEPAEIEAVLALHPAVREAAVV
                     VRQDAAGLRSLLGCVVPGAAVNAEELRAAVRGTLPDYMVPGAFALLEALPLTPSGKVD
                     RKALERMAPAREERAGEAEAVAPRTPSEEILASIFAEVLRHERVGIHDGFFDLGGHSL
                     LATQVVSR"
BASE COUNT          250 a          638 c          864 g          319 t
ORIGIN      
        1 aggagctggt cacggagcga ggcagcctgg agcactcgcc gctcttccag gtgatgctgg
       61 cggtgcagaa cacgcccacg gtcgcggaag gggtggtggc gccggggctg gagctgcggc
      121 cggtggcggc ggagtccgcg acggccaagt tcgagctgag cctgggcttc gctctgctcg
      181 gcgaggagct gacgggcggc ctggagtaca acaccgacct cttcgacgcc tccacggcgg
      241 agcggctggc gcggcacttc gcgcggctgc tggaggtggc gatggcggcg ccggagatgg
      301 cctggccgga gctgccgctg ctggaccggt ccgaggagca gcaggtgctg ctggagtgga
      361 acgacaccga agccgcctat ccaggtagcg ggcgctgtct gcacgagctg gtcttcgagc
      421 aggcggagcg ggcgcccgag gcggtggcgg tggtggccgg ggaggagggg gaggagcatc
      481 tgacccacgc tgggctgcgc cgccgggcgg gatggctcgc ccaccggctg cgcggcctcg
      541 gggttggtcg ggaggtgccg gtggcgctct gcgccgagcg gtcgctggag atggtcgtgg
      601 gtctgctggg catcctcgag gcgggtggag cgtacgttcc cctggatccc gactatcccg
      661 ccgatcgcct ggccttcatg gtggaggacg cggcggcgcc gctgctcctg gcgcaacgcc
      721 acctggtgga tcggctccct gagctgcgag gtgtgcggat cgtcttcctg gacgagctgg
      781 cgcgtccgga ggcgggcgcg ccgccggccg tgcccgatgg cgccgggccg gaccacctgc
      841 tgtacgtgat ctatacctcg ggctcgacgg gccgtcccaa gggggtgatg gtgcgccacc
      901 ggggcgtggt caaccgtctg ctctgggcgg cccacgccta tccggtgacg gcggcggacc
      961 gggtgctgca gaaggcgccg ttcagcttcg acttctcggt ctgggagtgt ttcggcacgc
     1021 tgctgtccgg ggcgcggctg gtgctggccc ggccgggtgg ccagcgcgac ggcgcctacc
     1081 tggcgcggac ggtggtggag caggggatca cgctgctgca cttcgtgccg gcgatgctgc
     1141 aggccttcct ggaggaggag gggctggatg ggtgccgcag cctgcgcttc gtcttctcgg
     1201 gcggcgacgc gctggcgtcc gagacggtgg agcggttcca ggcccggatg cctgccgggg
     1261 tgctgctgcg caaccagtac ggccccaccg agatctccat cgacgtcacc gagtggatct
     1321 gccgcgagga gcggaccgga aggacgccgc cgatcggccg tcctctaggc aacggccggg
     1381 tctatgtcac cgggcccaat ggggagctgg tgccggtcgg cgtagcggga gagctgctgc
     1441 tgggcggcgt gggagtggcc cggggctacc tgggtcggcc cgacctgacg gcggagcgat
     1501 tcgtgccctt tccctggcaa gacgaggcgg gggcgcgtct ctaccgcact ggggaccggg
     1561 tgcgtctgct ggcggacggc aatctcgagt tcctgggccg cgtcgatcac caggtcaaga
     1621 tccgtggcta tcgcatcgag ccggcggaga tcgaggcggt gctggcgctg catccggcgg
     1681 tgcgcgaggc ggccgtggtg gtgcggcagg acgcggcggg gctgcggtcg ctgctgggct
     1741 gcgtggtgcc gggggccgcc gtaaacgccg aggagctgcg ggccgcggtg cgcggcacgc
     1801 tgccggacta catggtgccg ggcgcgttcg cgctcctgga ggcgctgccg ctgacgccga
     1861 gcggcaaggt ggaccgcaag gctctagaga ggatggctcc ggcgcgtgag gagcgtgccg
     1921 gagaggcgga ggccgtggcg ccgcgcacgc catcggagga gatcctggcg tcgatcttcg
     1981 cggaggtcct gcggcacgag cgggtgggga tccacgacgg cttcttcgac ctaggggggc
     2041 actcgctgct ggcgacgcag gtggtgtcgc g
//
LOCUS       DASPJI010000824         5019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_51992, whole genome shotgun sequence.
ACCESSION   DASPJI010000824 DASPJI010000000
VERSION     DASPJI010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51992"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /gene="hflX"
                     /locus_tag="VEL74_23225"
     CDS             <1..780
                     /gene="hflX"
                     /locus_tag="VEL74_23225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HYG65513.1"
                     /translation="VELAQLKYDLPRLTEKDAGLSRLAGGIGGRGPGETVMEIGRRRI
                     RDRIRALEKQVESLSRQRDLRRRRRREEGVPVLSIIGYTNAGKSTLLNAMTGSEVEAA
                     DKLFMTLDPTSRRLRFPREGEVVITDTVGFIADLPKDLVAAFRATLEELADADLLLHV
                     VDVSDPQLEQKLEAVVRILEELKLSHVPRLLVLNKADLLPPYEAEAAARRHGGILVSA
                     IKRAGFADLIHAAEERLGREEPLVKTYEPKAEDKAEEVANA"
     gene            824..1966
                     /gene="hemW"
                     /locus_tag="VEL74_23230"
     CDS             824..1966
                     /gene="hemW"
                     /locus_tag="VEL74_23230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HYG65514.1"
                     /translation="MPFCSRICPYCDFAVLMGGRERREGFVRRLIEEIRLWREEWTAA
                     APFDTIYFGGGTPSILEPDQLALILDAARGLLPVADDAWIFLEANPEDVNPESLRAWR
                     ALGVRTLSLGVQSFDAETLRFLGRRHGPEQARRSVEAALAAGFDTVSVDLIYGSPGEE
                     PGSWRRSLEEAVVLGPDHLSCYQLTIHEGTPFGFRLERGKLAETPETAQAELFLFTHS
                     FLGDHGFPGYEVSNFARSPEHRSRHNRKYWDHTPYLGLGPSAHSFAAGPAPRRWWNER
                     KLGPYEMKVDLGEQPLAGAEELGPLDLALEALMLGLRTAGGIDLTRFRERYGVDLLER
                     NRPLLEQLAGENLARLEEGALRPTLAGFAIADSLARSFDLGGSDTG"
     gene            complement(2011..2649)
                     /locus_tag="VEL74_23235"
     CDS             complement(2011..2649)
                     /locus_tag="VEL74_23235"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HYG65515.1"
                     /translation="MKGRFWLAVLSMFVLLVTTGGLAAAQGTQEKAQEKPAGGAPQMS
                     PEEMQAMVAAATPGEAHKRMAQMAGNWTYTMTMWMAPGQPPMETTGTIESSMVLGGRY
                     LQSTYKGNIMGQPFEGRGTDAWDNVSKEYIGTWMDNMGTGIMVTRGKCDDPACKKMTA
                     TGEMNDPMTGGPVKVKMVTTHIDKDNFKFEMFMDHGEHGEMKTMEVNAKRKA"
     gene            2879..4855
                     /gene="parE"
                     /locus_tag="VEL74_23240"
     CDS             2879..4855
                     /gene="parE"
                     /locus_tag="VEL74_23240"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HYG65516.1"
                     /translation="MPTPNVYDSSAIEVLTGLEPVRKRPGMYTFTDRPDHLAQEVIDN
                     AADEAIAGHCKEILVTLHADGSLSVADDGRGMPVDTHPGEKVSGVEVILTRLHSGAKF
                     SDKSYRFSGGLHGVGVSVVNALAEKLEVWVKRDGKEHHIAFAGGEKTSDLQVVGTVGQ
                     RNTGTTVRFWPDAKFFDTPKFTVAKIKHSLRAKAVLSPGLHIRFVHEEQPDESDEWIY
                     EEGLTDYLMDEFAGCELIPAEPFVGKFRGKDEEVDWAVLWLLDDDMEMVTESYVNLIP
                     TVQGGTHVNGFRAGLTEAIREFCEFRDLLPRGVKLTPEDVWARCCYVLSLRMKDPQFT
                     GQTKERLSSREAAAFVAGVAKDAFALWLNQHVADAERIARLAIDSAQARLRAGKKVQR
                     KTVTTGPALPGKLSDCSSQDLSMTELFLVEGDSAGGSAKQARSREFQAILPLRGKILN
                     TWEVDPAEVLASQEVHDIAVALGVDPGSDKLDGLRYGKICILADADSDGSHIATLLCA
                     LFFRHFRQLVKDGRVYVAMPPLYRIDVGKEVYYALDEREKKGILDRIEAEKIKGKVNV
                     QRFKGLGEMNPMQLRETTMAPETRRLVQLVLEEPPHIADGDGTEVTTDTALATETNGA
                     GNAEAVIDRLLAKARSADRREWLMAVGDRAEVEV"
BASE COUNT          785 a         1783 c         1643 g          808 t
ORIGIN      
        1 gtcgagctgg cgcagctcaa gtacgacctg ccgcgcctga ccgagaagga cgccggcctg
       61 tcgcgtctgg ccggcggcat cggcggccgg ggccccggcg agacggtgat ggagatcggg
      121 cgccggcgga tccgcgaccg catccgggcg ctcgagaagc aggtggagag cctctcccgc
      181 cagcgcgacc tgcgccggcg ccggcgccgc gaggaggggg tgcccgtgct ctcgatcatc
      241 ggctacacca acgccggcaa gagcacgctg ctcaacgcca tgaccggcag cgaggtcgag
      301 gcggcggaca agctgttcat gacgctcgac ccgaccagcc ggcgcctgcg cttcccgcgc
      361 gagggcgagg tggtgatcac cgacaccgtc gggttcatcg ccgacctccc caaggatctg
      421 gtcgccgcct tccgggcgac gctcgaagag ctggccgacg ccgacctgct gctccacgtg
      481 gtcgacgtct cggacccgca gctcgagcag aagctcgagg ccgtggtgcg gatcctcgag
      541 gagctgaagc tctcgcacgt gccgcgtctc ctcgtcctca acaaggcgga cctgctgccc
      601 ccctacgaag ccgaggcggc cgcccgccgc cacggcggca tcctggtgag cgccatcaag
      661 cgcgccggct tcgccgacct catccacgcc gccgaggagc ggctgggccg cgaggagccg
      721 ctggtgaaga cgtacgagcc caaggccgag gacaaggccg aagaggtggc gaacgcctga
      781 tccctcgtct gtcgcgagtg gggggcccgg cctctacctc cacatcccct tctgctcccg
      841 gatctgcccc tactgcgact tcgccgtgct catgggcggc cgggagcggc gggaggggtt
      901 cgtccgccgc ctgatcgagg agatccggct ctggcgggag gagtggaccg ccgccgctcc
      961 gttcgacacc atctacttcg gcggcggcac gccctccatc ctggagccgg accagctcgc
     1021 cctcatcctc gacgccgccc ggggcctcct cccggtggcg gacgacgcct ggatcttcct
     1081 cgaagccaat ccggaggacg tgaatccgga aagcctccgc gcctggcggg cattgggggt
     1141 ccgtaccctc tccctcggtg tccagtcgtt cgacgcggag acgctgcgct tcctcggccg
     1201 ccggcatggc cccgagcagg cgcggcgtag cgtcgaggcg gcgctcgcgg ccggcttcga
     1261 caccgtctcg gtcgacctga tctatggctc gcccggggag gagcccggct cctggcggcg
     1321 ctcgctggag gaggcggtcg tcctggggcc ggaccatctc tcctgctacc agctcaccat
     1381 tcacgagggg acgccgttcg gcttccggct ggaacgggga aagctcgccg agacaccgga
     1441 gacggcgcag gcggagctgt tcctcttcac ccactcgttc ctcggggatc atggcttccc
     1501 cgggtacgag gtctccaact tcgcccgctc cccggagcac cgctcgcggc acaaccggaa
     1561 gtactgggac cacactccct accttggtct cggtccctcg gcccactcct tcgccgccgg
     1621 accggccccc cgccgctggt ggaacgagcg caagctcggc ccctatgaga tgaaggtgga
     1681 cctcggggag cagccgctcg ccggcgccga ggagttgggc ccgctggacc tcgccctgga
     1741 ggcgctcatg ctgggcctgc ggaccgccgg cggcatcgac ctgacccggt tccgggagcg
     1801 ctatggcgtg gacctcttgg agcggaaccg cccgctgctg gagcagttgg ccggggagaa
     1861 tctggcgcgc ctggaagagg gggccctccg gccgactctc gcggggttcg ccatcgccga
     1921 ctcgctggcg cgctcgttcg atctgggagg gagcgacacc gggtaacggc ggggtgacgg
     1981 cgagggccgc caccccgaag ctagaaagga tcaggccttc cgcttggcgt tgacctccat
     2041 ggtcttcatc tcgccatgct cgccatggtc catgaacatc tcgaacttga agttgtcctt
     2101 gtcgatgtga gtggtgacca tcttgacctt gacgggcccg ccggtcatcg ggtcgttcat
     2161 ctcgcctgtg gcggtcatct tcttgcacgc cgggtcgtcg cacttccctc tcgtgaccat
     2221 gatgccggta cccatgttgt ccatccaggt gccgatgtac tccttgctga cattgtccca
     2281 tgcgtcggtg ccgcgcccct cgaacggctg gcccatgatg ttgcccttgt aggtagactg
     2341 gagatagcgg ccgccgagga ccatggacga ctcgatggtg ccggtagtct ccatgggggg
     2401 ttgacccggc gccatccaca tggtcatcgt gtaggtccag ttgccggcca tctgcgccat
     2461 ccgcttgtgc gcctcaccgg gagtggccgc ggccaccatc gcctgcatct cctccgggct
     2521 catctgcggg gctccgcccg ccggcttctc ctgcgccttc tcctgcgtcc cctgggcggc
     2581 ggccagcccg ccggtcgtca ccagcaatac gaacatcgag agcactgcaa gccagaatcg
     2641 tcccttcatg gttgcctctc ctttcaagtt ggaactgcga gtttaccgcc ggatggtacg
     2701 gcgtgggagc ccggggcggg gaagatttcc ccattacgct ccgtttgcct ggaaggctat
     2761 caacggaccc ggcatcctgt caacgccccg gttacgccga aaggtgctcc tgaagggccg
     2821 gcctcttgta tgatgggccc gctcgaaccg aaccgctccc gaggtgcagc cagtctccat
     2881 gccgactccg aacgtctacg attcgtccgc cattgaagtc ctgaccgggc tcgagccggt
     2941 gcgcaagcgc cccggcatgt ataccttcac cgaccgcccc gaccacctgg ctcaggaggt
     3001 catcgacaac gccgccgacg aggcgatcgc cggccactgc aaggagatcc tcgtcaccct
     3061 gcacgccgac ggctcgctgt cggtggccga cgacggccgc ggcatgccgg tcgataccca
     3121 ccccggcgag aaggtctcgg gcgtcgaggt gatcctcacc cgcctgcact ccggcgccaa
     3181 gttctcggac aagagctatc gcttctccgg cggcctccac ggtgtcggcg tctcggtggt
     3241 caacgccctc gccgagaagc tggaggtctg ggtcaagcgc gacggcaagg agcaccacat
     3301 cgccttcgcc ggcggcgaga agacctcgga cctccaggtg gtcggcacgg tcgggcagag
     3361 gaacaccggc accacggtgc gcttctggcc cgacgccaag ttcttcgaca cccccaagtt
     3421 caccgtcgcc aagatcaagc actccctgcg cgccaaggcg gtgctctcgc cgggcctcca
     3481 catccgcttc gtccacgagg agcagcctga cgagagcgac gagtggatct atgaggaagg
     3541 gctcaccgat tacctgatgg acgagttcgc cggctgcgag ctgatcccgg ccgagccgtt
     3601 cgtcggcaag ttccgcggca aggacgagga ggtcgactgg gccgtcctct ggctgctgga
     3661 cgacgacatg gagatggtga cggagagcta cgtcaacctg atccccaccg tccagggggg
     3721 cacccacgtc aacggtttcc gcgccggcct caccgaggcg atccgcgagt tctgtgagtt
     3781 ccgcgacctg ctgccgcgcg gcgtcaagct gacccccgag gatgtctggg cccgctgctg
     3841 ctacgtgctg tcgctgcgca tgaaggaccc gcagttcacc ggccagacca aggagcggct
     3901 ctcctcgcgc gaggcggcgg cgttcgtcgc cggggtggcc aaggacgcct tcgccctctg
     3961 gctcaaccag cacgtggccg acgccgagcg catcgcccgt ctggccatcg acagcgccca
     4021 ggcgcggctg cgggccggca agaaggtgca gaggaagacg gtgaccaccg ggccggcgct
     4081 ccccggcaag ctctccgact gcagcagcca ggatctctcg atgaccgagc tcttcctggt
     4141 ggaaggcgac tcggccggcg gctcggccaa gcaggcgcgg agccgcgagt ttcaggccat
     4201 ccttcccctg cgtggcaaga tcctcaacac ctgggaggtg gacccggcgg aggtgctcgc
     4261 ctcgcaggag gtgcacgaca tcgccgtcgc cctgggcgtc gatcccggct cggacaagct
     4321 tgacggcctg cgctacggca agatctgcat cctcgccgac gccgattccg acggctccca
     4381 catcgcgacg ctcctctgcg ccctcttctt ccgtcacttc cggcagttgg tgaaggacgg
     4441 ccgggtctac gtcgccatgc cccccctcta ccgcatcgac gtcggcaagg aggtctacta
     4501 cgccctcgac gaacgggaga agaagggaat cctcgaccgc atcgaggccg agaagatcaa
     4561 gggcaaggtc aacgtccagc gcttcaaagg cttaggcgag atgaacccga tgcagctccg
     4621 cgagaccacc atggctcccg agacccggcg cctggtccaa ctcgtcctcg aggagccccc
     4681 tcatatcgct gatggcgacg gcaccgaggt caccaccgat accgcgcttg ccaccgaaac
     4741 gaacggtgcc ggcaatgccg aggcggtcat cgaccggctg ctcgccaagg cccgctcggc
     4801 cgaccgccgc gaatggctga tggccgtggg cgaccgggcc gaggtggagg tctagaccat
     4861 ggccagggtt tccgcacgca ggaagtccgg tagcggcgcc aatggccggg ggctcgtcgc
     4921 ccgcctgccg ttcaacgagg tcgagaagct gtcgctcgcc acgttcaccg agaaggcgta
     4981 cctcgactac tcgatgtacg tcatcctcga ccgcgccct
//
LOCUS       DASPJI010000825        17284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_52048, whole genome shotgun sequence.
ACCESSION   DASPJI010000825 DASPJI010000000
VERSION     DASPJI010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17284)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17284
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52048"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VEL74_23245"
     CDS             complement(<1..952)
                     /locus_tag="VEL74_23245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HYG65517.1"
                     /translation="MTDLAALPPPALAPEKPSRPSLAARIPAGGTSDPDEILDLFLGW
                     VSDLGLELYPAQEEALLEIMAGRHVILNTPTGSGKSLVALGVHFKGLCEGKTSFYTSP
                     IKALASEKFFSLCEDLGPENVGMLTGDASINATAKVLCCTAEVLSNMALRRGETLDAP
                     YVVMDEFHYYSDAERGVAWQIPLITLPHTRFLLMSATLGDVSAIAERIEEQTSADVAL
                     VSSEQRPVPLDWEYRETPLHETVEYLLTSRKAPVYIVNFTQRECAELAQALTSTQIAN
                     REERDRIRDAIGGYRLDTPYGKEFRRFLSFGIGVHHAGLLP"
     gene            1108..1938
                     /locus_tag="VEL74_23250"
     CDS             1108..1938
                     /locus_tag="VEL74_23250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65518.1"
                     /translation="MRSRRVLALLACSLLLGLTPLAADSVIQRGTDVFTTMGGKTYYD
                     FAEVPIPAGFFCKGSKPFTGRVAFKGLPLATETPGQLQGGDTVIERLDDAVFDAKGVA
                     TTRIRFRALSMVSIEPVKTRCGAFHAYVSLAGRQRVTTMKIRRTEEGGGTFAAPLAVD
                     VRLTFVPVKPPRNGKARKLELTGSFTFPANPVPWSYGTEAMAKRVTAGVIDTNGDQTP
                     DTLVPGTSNFVAGWTPGAQNKACCREWVCHAESSGKEHCYWQPPPPECWNMPCTIDMY
                     "
     gene            complement(2025..3464)
                     /locus_tag="VEL74_23255"
     CDS             complement(2025..3464)
                     /locus_tag="VEL74_23255"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HYG65519.1"
                     /translation="MSKAVWRRFLVPSCLVAALAASSAPPAGAAGPFYDEMDMQVVSE
                     GFTAEKQLERQATLSRRLLRELPQIAEQKAIRIQLSADEINGVDKASRKTSPLRIGLV
                     KPMQPIVVSGLDGDSLEKAASGRLVWAAVVRANNAGAIRLHIEDMSLPRNAELFAYSR
                     AGQAYGPYTGTGPDRTGEFWTTTIFGSEAILQLRLNGPVSDEDLRAISFKVREAGVIT
                     EGFAGTLGGTKPPMPWEKAYCGNASCIVDATCFTNTPAEPQKLATAKMEWISGRYIYT
                     CTGSLIADNNPTRDNFFLTANHCLSASKAAKNVNFYWRFASSSCNGGCPSNNGWPYIT
                     TGSSVVSSNRTADYTLLQLTSNPPAGSVRLGWNSTPIANTNGAALYRVSNPGFGPQVY
                     SAHTVDTTAGTCTGWPRGSWIYSRDITGATDGGSSGSPVLNANSQVVGQLSGGCGFNV
                     NDVCDVQSNATVDGAFAAYYNTIRPYINP"
     gene            complement(3609..4844)
                     /locus_tag="VEL74_23260"
     CDS             complement(3609..4844)
                     /locus_tag="VEL74_23260"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HYG65520.1"
                     /translation="MPGRPRRSRKRGPLAGVRVVDASRVLAGPYLAMLLGDLGADVVK
                     VEKPDGGDQTRGWGPPFVGPPGRQVSAYFISANRNKRSVALDLKSPAGRAAFDRLLAG
                     ADVLVENFLPAEWRKLGFRGRDQTRRHPRLVQCTVSGYGTAGPEADRPAYDVVLQAES
                     GLMSITGFGDGEPVRVGVAVIDFLSALYGLGGLLAALRLRDVPGRGRRVEVSMVDAGA
                     SFLSYAAQSWLADGRQPPALGSRHPNLAPYQAFRAADGWLIVGVGSQDLWRRFCRAIE
                     RPGLADDPRFRTSSDRVRNRDGLDALLAGVFAAHPTAHWDRVLREHRVPAGPTASVGE
                     AVEKARRRGQVVDLPTGAYGPLPTVASPFLIDGERALPGQPPPALGEHTEEVLREAGL
                     SREEVRDLLRTGVAAGALG"
     gene            4909..5247
                     /locus_tag="VEL74_23265"
     CDS             4909..5247
                     /locus_tag="VEL74_23265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYG65521.1"
                     /translation="MIVQKRHLDGFTLLNVEGVIKLGESAQFFAQTLDRALADDRGHV
                     IVDFTKINSIDSTGIGELVGYLGRFRSRNRELILVNPSERIRKLLQVAGLSQLFPIYE
                     TVEAAVAAER"
     gene            5275..6303
                     /locus_tag="VEL74_23270"
     CDS             5275..6303
                     /locus_tag="VEL74_23270"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HYG65522.1"
                     /translation="MRNGLPESWHSGAVAAVTPEGRLVASLGDPEVATFLRSAAKPFQ
                     CLPLLLAGGEARFGLDAADIALTCASHGGTPAHVRRAGDLLARGGFGIGDLLCGAHPP
                     MDAESARRLVLAGAEPTPLHNNCSGKHAGMLLACRLLGLPTKGYIAPDHPLQRRALDH
                     TARFCGVAPESIGLATDGCSVPTYHLSLAAAARGYAALAHPEAAGLDGEVAAAVRRVV
                     AAMTGAPEVVAGPGRFTTRLMEATGGRVLGKEGAEGFYGVAVRGPVALGIAVKIADGG
                     ERCRDGVVLDVLRQLGSLSGAEFETLAPFYRPRLVNWAGLPAGEVRPDLELEEAELEE
                     AELEEEEV"
     gene            6325..6873
                     /locus_tag="VEL74_23275"
     CDS             6325..6873
                     /locus_tag="VEL74_23275"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HYG65523.1"
                     /translation="MTRTTASAPTLFGPTLRRGLCLLLALLAGGAALVTLAGCRSSRG
                     KAQEEGGRRPLYRKVSPPVAFEIIRDTPETLILDLRTPEEYLGETGHLRRALNIPLDR
                     LPYRILEISAWRQETVLVYCRASDPCGEEGMAVLVSSGFEDAMLIDGGIDGWIEEGFK
                     TELPAGLAGQGPVPPPPPPLVP"
     gene            6870..7904
                     /locus_tag="VEL74_23280"
     CDS             6870..7904
                     /locus_tag="VEL74_23280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65524.1"
                     /translation="MKKTAGLLAAALLPLLAFFPAAAQRPAALPYADAIRLAEAFRLA
                     ETVQERVFPGWSRAPFSVLLASGERDLLVRHPEPSAEFSELGYSSILRERVFTRPGRT
                     APDLLATFPVIGASPTVVVGSAERTGLRSTEWVLTVLHEHFHQFQMADPGYFAAVERL
                     GLAGVDRTGMWMLNYPFPYDAPATAARFADLSRRLAALLRQGADAAPATVEAFWRDYR
                     AFLAGLGEKDRLYFSFQIWQEGVPRYIELRSAQVAAEAYEPSLEFRALPDARGFDEVA
                     GGLRTDILAQLDSANLGRDRRVAFYAFGAGLCLLLDRSNPDWKSRYMAERFSLERSAG
                     VPSGRRESRR"
     gene            8005..9444
                     /locus_tag="VEL74_23285"
     CDS             8005..9444
                     /locus_tag="VEL74_23285"
                     /inference="COORDINATES: protein motif:HMM:NF020826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HYG65525.1"
                     /translation="MTVHAPISTAESLRELKEALGDRILLDEEVRAQFRSDFGRYVDR
                     LPGAVARCMSPEEVAQVIRFCAERGIPVVARGQGHTQTGQATTEGGVLLDTSSMQTIH
                     EIDEENETATVDGGVVWRSLVRATVPKGLVPRVLTNNLGVAIAGTLSMAGLGVASFRY
                     GTQADNAVELVVVTGTGDIVTCSREQNSDLFDVARCGLGQFGVIVRAKVRLRRCQSMV
                     RKYYLLYDDLGVLMEESKRIMDPSNATFSSLESWCTPCFQGIKKIGEGMELAEGVQTF
                     AYWMYPLHLTVEFEPGQEPDDQAVLAGLTPYRHLETSDFTQLEFCERMDPVFELWRRG
                     GYWDMAHPWMETTLPWDTAREFIESALAITPPQALGPGGHILLWPAYTRTSEVPLFMH
                     PEGEYVMGWGILPGVPFKFLDRALAQLDMASDLSIAYGGKRYLSGYITFNTAEKWAAH
                     FGDRWPMILAAKKKFDPAGIMAPGFIQYE"
     gene            complement(9520..10521)
                     /locus_tag="VEL74_23290"
     CDS             complement(9520..10521)
                     /locus_tag="VEL74_23290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65526.1"
                     /translation="MNLRHRSLTKIFGPVLLTTTLVLPVELLATPSAAFAAVQMERGE
                     KKNERNKRQNQAQGRGRAQQQRSQAKQQREQAKQNRGQQRRQVQAQAQQRRDAERLQQ
                     QQRKNQVKLQAQQQREARLRQQQRQNQIKLQAQQQRQAQLQRQQYQRQVQLQRQAEIQ
                     RQAELRRQAELRRQAQVRRDNRNYYPRDRQVYNNNRYNDNRYYRDYQRNGGRYRYDGR
                     YYGANERFHIDGFVVDEGSGCSLFKDHQTGEMFALVGNIGDVQQGDHMRLQGRVVDGG
                     VCDWRGTGFEVIQVKTLWDTNRHESASYDFQRDRITFEQYLDRHDYRDDRYGYGDWR"
     gene            10733..11596
                     /locus_tag="VEL74_23295"
     CDS             10733..11596
                     /locus_tag="VEL74_23295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HYG65527.1"
                     /translation="MTEALSTPTTTIRIAHSPDSDDAFMFYALTQGLLDTEGLAIEHV
                     LRDIETLNQAAFQGTYEITALSFHGYAHLADRYQLMPSGASFGDGYGPVVVSRERLGR
                     ADLVGCKVAIPGQLTTAHLALRLWQPEVEVQIVPFDQILDVVQAGDAEAGVVIHEGQL
                     TYQEMGLRSVVDLGAWWKEETGLPLPLGGNGIRRDIDPELKLRLCRLLTQSIEYGLDN
                     RQEALGYAIRYARGLEDDLERSDRFVGMYVNDWTRGYGEAGRRAVQLLLDRGHEAGVL
                     PRRIEAEFIEI"
     gene            11605..12291
                     /locus_tag="VEL74_23300"
     CDS             11605..12291
                     /locus_tag="VEL74_23300"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HYG65528.1"
                     /translation="MLRPAALVFDLDGTLIDSRRDITTAINRMRADLGRPPLTLEQVV
                     TMVGEGAKLLVFRALGGDVPEQILERALSTYLEHYRDVCLETTQPYPGVEALLESVAA
                     RYPMALLSNKGEALSRKILDGLNLSRFFREILGGDSLPTRKPDPGGLLHLARRLQVPP
                     ERLLMVGDTWVDAVTARNAGCPFGLVEWGFPRTPAPEELQADLRTADPDELRVRLLSL
                     SGQAPEPKPS"
     gene            12318..12923
                     /locus_tag="VEL74_23305"
     CDS             12318..12923
                     /locus_tag="VEL74_23305"
                     /inference="COORDINATES: protein motif:HMM:NF016356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HYG65529.1"
                     /translation="MADTLSPSLPLSEPRRVRVGISACLLGEEVRYNGGHKRDFLLLD
                     ALGRFVEWVPVCPEVEIGLGVPRPPIRLARIAEGDDGVRLVMPGRGEDLTDLMVSYAE
                     RRVDGLARLGLAGYVLKKDSPSCGMERVTVWPAGGAPAEEGRGLFAEVLMRQLPDLPV
                     EEEGRLRDPMERDHFLSRIYAYDRRLRANEDRPTARQPSTP"
     gene            13021..13911
                     /locus_tag="VEL74_23310"
     CDS             13021..13911
                     /locus_tag="VEL74_23310"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HYG65530.1"
                     /translation="MKKLIRTSVLAPALAAIIAVLSAGAAPCLAGIHYKAVTRNEGGQ
                     EKTPDFQVEGWVSGDKARVEFIGANPSGAKGAYLITRDGGKVVYLVNPEEKSYAEWDM
                     QAMLGLAGSIMQGMGPLLKFEVSDPKVEKLAEEDGGSILGVPTRHVRYRTSYTMKVKV
                     LGMGNVSDVVSEQDIWTSDRLQDRALSVWLRSEPPRSGNEQLDKLIASQYKVQGFPLK
                     MVTVTTSTPQKKGQPTVTRSTMEVTQLDTSATVPDSRFEIPAGYQEAQMVPAGEGDEA
                     EQGGPLGGLLRRKKSNSGGN"
     gene            13922..14416
                     /locus_tag="VEL74_23315"
     CDS             13922..14416
                     /locus_tag="VEL74_23315"
                     /inference="COORDINATES: protein motif:HMM:NF027592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HYG65531.1"
                     /translation="MRSRALMLAVAAAVLLPVLAWADSPAKGETYGKGVSLTQTTSIQ
                     EILARPDDFVGKTVRIEGPVLDVCPMKGCWMELGAQQADGTASLRVKVEDGVIAFPVT
                     AKGKLAVAEGTVEAIPMTREKYVDWLAHLSEERGEKLDPSTVGEGPFRILQIQGTGAR
                     IEGP"
     gene            14413..14985
                     /locus_tag="VEL74_23320"
     CDS             14413..14985
                     /locus_tag="VEL74_23320"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HYG65532.1"
                     /translation="MSEREDEAGGARHLPGGERLGSREIFRGRTVHLDVDHVRLPNGN
                     EMDLELIHHPGAAAVVPVLEGDVLMVRQYRYATGGWLLEVPAGKLDPGESPKSCAFRE
                     VEEETGYRPTDLQPLGWIWTSPGFTDEKIWLYLATGLTATRQQLGKDEVLSLERLPLR
                     EAIDKAARGEIHDAKSVCALLRAARHLDHQ"
     gene            15048..16010
                     /locus_tag="VEL74_23325"
     CDS             15048..16010
                     /locus_tag="VEL74_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HYG65533.1"
                     /translation="MRSAILTEPRRFDIQSMEPPQPGEGEVRLRVLGCGVCGSDMGPW
                     KGLHGLTYPMPAGAPGHEVFGTVESLGPGVQGLQVGMPVTALTYRAYSEYDIAKADSL
                     VPLPPSLAGRPVLGEPVACAVNVVRRSGVREGDTVVLLGTGFLGALVLQLLRRERPGR
                     VFAVSRRPLSPGLAERFGADEVLTYDDDVRGRVGAATGGQMADVVIEATGYQMPLDLG
                     AELTRIRGRLLIAGYHQEGPRTVNMQLWNWRGLDVINAHERDPEIYRSGMEEGVRRLA
                     AGEIDLAPLITHSFPLADINQAFRTAEERPAGFLKSLVFTGASA"
     gene            16007..16999
                     /locus_tag="VEL74_23330"
     CDS             16007..16999
                     /locus_tag="VEL74_23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017967241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HYG65534.1"
                     /translation="MTPRLGFAGLGWIGAMRLDAVAAVGAGEIAALCEPTEARLEEVA
                     RAHPGAAAFTSLEEMLSRSGDLGLDGVVIATPNSLHAPQTLAALDRGLAVFCQKPLAL
                     SGQEAREMVDAARRADRLLGIDYSYRFTEGMKALRGMVARGELGRVFSVDTVFHNAYG
                     PDKSWCRDPRLAGGGALMDLGVHLVDLALWVLDDVAVEAVHGGVWSGGEAIHGLGIDD
                     FASARLELAGGAAVSLAVSWNAHAGQDCVIRLAAFGTSGGAEFRNVGGSFFDFELVRF
                     HGRSGEVVVRESRDWLGKAILDWTGRLGQGGRFDPEIERSVTVSRAVDALYGRD"
     gene            complement(17057..>17284)
                     /locus_tag="VEL74_23335"
     CDS             complement(17057..>17284)
                     /locus_tag="VEL74_23335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65535.1"
                     /translation="LFALMSNQKQIPAQPIRFKIVDEQTLSYERRSALERPGPRYAPA
                     EVNDRLKGFHVDRSFLLRHGIQEYLYVRDVA"
BASE COUNT         2485 a         5904 c         6147 g         2748 t
ORIGIN      
        1 tggggagcag cccggcgtgg tggacgccga tgccgaacga gaggaagcgc cggaactcct
       61 tgccgtacgg ggtgtcgagc cggtagccgc cgatggcgtc gcggatgcgg tcgcgctctt
      121 cgcggttggc gatctgcgtg ctggtgagcg cctgggcgag ctcggcgcac tcgcgctggg
      181 tgaagttgac gatgtagacc ggcgccttac gggaggtcag gaggtactcg accgtctcgt
      241 ggagcggcgt ctcgcggtac tcccagtcca gtgggacggg gcgctgctcg gagctgacca
      301 gggcgacgtc ggcggacgtc tgctcctcga tccgctcggc gatggcgcta acgtcgccga
      361 gggtggcgga catcagcagg aaccgggtgt gggggagggt gatcaggggg atctgccagg
      421 cgacgccccg ttccgcgtcc gagtaatagt ggaactcgtc catcaccaca tagggggcgt
      481 cgagggtctc cccccggcgc agcgccatgt tggacagcac ctcggcggtg cagcagagca
      541 ccttggcggt ggcgttgatg ctggcgtccc cggtgagcat gccgacgttc tcgggcccga
      601 ggtcttcgca cagggagaag aacttctcgc tggccagcgc cttgatcggg ctggtataga
      661 aggacgtctt cccctcgcac agccccttga agtggacccc cagggcgacc agcgacttgc
      721 ccgagccggt gggcgtgttg aggatcacgt gccgcccggc catgatctcg agcagcgcct
      781 cctcctgcgc cggatacagc tccagcccga ggtcggacac ccaccccaga aacaggtcga
      841 ggatctcgtc cggatcggac gtcccccctg ccgggatgcg cgccgccaga gaagggcgcg
      901 agggcttctc gggcgcgaga gccgggggcg ggagggcggc gaggtcggtc atatggtgct
      961 ggattgtcgc acgaaggcac cgaaccgact gtgcggcgcg ccgggcgggc ctctccgtac
     1021 cttcttagat agggacgcgg ttatcggcgg gaaggagcct gccggtggcg cgtccctcta
     1081 tcgtcctatg gggacaagga ggttctcgtg agaagtcgaa gggttctggc tttgctggcc
     1141 tgttcgctcc tgctcggtct gactccgctg gccgccgact cggtcatcca gcgtggcacc
     1201 gacgtcttca ccaccatggg aggcaagacc tactacgatt ttgccgaggt cccgatccct
     1261 gccggcttct tctgcaaggg ctccaagccg ttcaccggcc gggtggcctt caagggcctg
     1321 cccctcgcga ccgagacccc ggggcagctc cagggtggcg acaccgtcat cgagcggctc
     1381 gatgacgcgg tcttcgatgc caagggcgtc gccacgacgc gcatccgctt tcgggccctg
     1441 tccatggtca gcatcgagcc ggtcaagacc cgctgcgggg ctttccatgc ctatgtctcc
     1501 ctcgccggcc ggcagcgcgt gacgacgatg aagatccggc gcaccgagga ggggggcggg
     1561 accttcgcgg ctcctctggc ggtcgatgtg cggctgacgt tcgtcccggt gaagccgccg
     1621 cggaacggca aggcccgcaa gctggagctc accgggagct tcaccttccc ggccaatccg
     1681 gttccctgga gctacgggac ggaggcgatg gcgaagcgtg tcaccgccgg ggtcatcgac
     1741 accaatggcg accagacgcc cgataccctg gtccccggca cctcgaactt cgtggccggg
     1801 tggacgccgg gagcgcagaa caaggcgtgc tgcagagagt gggtatgcca cgcggagtcc
     1861 agcggcaagg agcactgcta ctggcagcct cctccgcccg agtgctggaa catgccctgc
     1921 acgatcgata tgtactaggc cgcaaacagg actgagcctc tgtagcatcg aggccgttcc
     1981 cggcctgggg ttactccccc aggccgggaa cggccgctgc ggaactacgg gttgatgtag
     2041 ggccggatgg tgttgtagta ggcggcgaag gcgccgtcca cggtggcgtt cgactgaacg
     2101 tcacagacgt cgttcacgtt gaagccgcag ccgccgctca actggccgac gacctgcgag
     2161 ttggcgttca ggaccggcga gccgctgctg ccgccgtcgg tggcgccggt gatgtcgcgg
     2221 ctgtagatcc acgagccgcg cggccagccg gtgcaggtcc cggcggtggt gtccacggtg
     2281 tgcgccgagt agacctgggg cccgaagccg gggttgctca cccggtagag ggcggcaccg
     2341 ttggtgttgg cgatcggcgt gctgttccag cccaggcgga ccgagccggc cggcgggttc
     2401 gacgtcaact ggagcagcgt gtagtccgcc gtccggttcg agctgacgac cgacgacccc
     2461 gtcgtgatgt acggccagcc gttattgctg gggcagccgc cgttgcagga ggaggaggcg
     2521 aaccgccaat agaagttgac gttcttggcg gccttgctgg cgctcaggca gtgattggcg
     2581 gtcaggaaga aattgtcccg ggtggggttg ttgtcggcga tcagggaacc ggtgcaggta
     2641 tagatatacc ggccggagat ccattccatc ttcgcggtgg ccagcttctg gggctccgcg
     2701 ggggtattgg tgaagcaggt ggcgtcgacg atgcaggagg cattaccaca gtaggccttc
     2761 tcccagggca tcggcggctt ggtgccaccc agcgtgccgg cgaacccttc ggtgatcacg
     2821 ccggcctcac gcaccttgaa ggagatggcg cgcagatcct cgtcgctcac cgggccgttg
     2881 agccggagct gcaggatcgc ctcggagccg aagatcgtcg tcgtccagaa ctcgccggtc
     2941 cggtcggggc cggtgccggt ataggggcca taggcctggc cggcgcggct gtaggcgaag
     3001 agctcggcat tgcgtggcag ggacatgtct tcgatatgca ggcggatggc gccggcattg
     3061 ttggcgcgca ccacggcggc ccacactagc cggccgctcg ccgccttctc cagcgagtcg
     3121 ccgtccaggc cggagaccac gatgggctgc atgggcttga ccagaccgat cctgagcggg
     3181 gaggtcttgc gcgaagcctt gtcgactccg ttgatctcgt cggccgagag ctggatccgg
     3241 atcgccttct gctcggcaat ctgcggcagc tcacggagca gccgccggct cagggtggcc
     3301 tgacgctcca gctgcttctc ggcggtgaag ccttcgctca ccacctgcat gtccatctcg
     3361 tcatagaacg gacctgccgc tccagccggg ggcgctgagc tggcggcgag ggctgcgacg
     3421 agacacgacg ggaccaggaa cctcctccaa actgcttttg acatccggtg ctcctttcga
     3481 cagggttcat tgatctgtcg acagcggcgc cgccttcccc ccacgagacg ccccccagcg
     3541 cccgatgagc cgtaccgcca tcctccaact gaagctatag gtgcgggcgg gtaccaaaag
     3601 cgggatcgct agcccagggc gcctgccgcc acccccgtcc tcagcaggtc ccggacctcc
     3661 tcccggctga gccccgcctc ccgcagcacc tcctcggtgt gctcgccgag ggccgggggc
     3721 ggctggccgg ggagcgcgcg ctcgccatcg atcaggaagg gggaggcgac ggtggggagg
     3781 gggccgtagg cgccggtggg gaggtccacc acctggccgc gccgccgggc cttctcgacg
     3841 gcctccccta cggaggccgt cgggccggcc gggacgcggt gctcgcgcag gacccggtcc
     3901 cagtgggcgg tgggatgggc ggcgaagacc ccggcgagga gggcgtcgag cccgtcccgg
     3961 ttgcggaccc ggtcggagct ggtgcggaag cgcgggtcgt ccgccaggcc gggccgttcg
     4021 atggcccggc agaagcggcg ccacagatcc tggctgccca cgccgacgat gagccagccg
     4081 tcggcggcgc ggaaggcctg gtagggggcg aggttggggt ggcgcgagcc cagggccggc
     4141 ggctggcggc cgtcggcgag ccaggactgc gcggcatagg agaggaagga ggcgccggcg
     4201 tccaccatgg agacctcgac ccgccgcccc cgcccgggca cgtcgcggag gcgcagggcg
     4261 gcaaggaggc cgccgaggcc gtagagcgcc gacaggaaat cgatgaccgc cacaccgacc
     4321 cgcaccggct ccccgtctcc gaagccggtg atcgacatga gcccggactc ggcctggagc
     4381 accacgtcgt aggcgggccg gtccgcctcc gggccggcgg tgccgtagcc gctcaccgtg
     4441 cactggacga gcctggggtg gcgccgggtc tggtcccgcc cgcggaagcc gagcttgcgc
     4501 cactccgccg gcaggaagtt ctccaccagg acgtccgccc cggccagcag ccggtcgaag
     4561 gcggcgcggc cggccgggct cttgaggtcc agggcgaccg agcgcttgtt gcggttcgcc
     4621 gagatgaagt aggcgctcac ctgccggccg ggcggcccga cgaagggggg accccagccg
     4681 cgggtctggt cgccgccgtc cggcttctcg accttgacca cgtccgcccc caggtcgccc
     4741 agcagcatgg cgaggtaggg tccggcgagg acgcgcgagg cgtccaccac ccgcacgccg
     4801 gcaagcggtc cccgcttcct ggatctcctg ggtctcccgg gcacgggcgg atggtagcag
     4861 gggcgccggc cagccgtgct agactgagcc cacctcaagc gtgccggcat gatcgtccag
     4921 aagcgccatc tcgacgggtt caccctcctc aacgtggagg gtgtcatcaa gctgggggag
     4981 agcgcccagt tcttcgccca gacgctcgac cgtgccctcg ccgatgaccg cgggcacgtc
     5041 atcgtcgact tcacgaagat caactccatc gattccaccg gcatcggcga gctggtcggc
     5101 tacctgggac ggttccgcag ccgcaaccgc gagctgatcc tggtcaaccc gtcggagcgg
     5161 atccgcaagc tgctgcaggt cgcggggctt tcccaactgt ttcccatcta cgaaaccgtg
     5221 gaagcggcgg tcgccgcgga gcggtaggtc ctgttcgatc gcgtcgcgac ggtcgtgcgc
     5281 aacggtctgc cggagtcctg gcactccggc gccgtggccg ccgtcacgcc ggaggggcgc
     5341 ctggtggcca gcctcggcga tccggaggtg gccaccttcc tgcgctccgc cgccaagccg
     5401 ttccagtgcc tgccgctgct cctggccggc ggcgaggccc gcttcggcct cgacgccgcc
     5461 gacatcgccc tgacctgtgc ctcccacggc ggcaccccgg cccacgtccg gcgcgccggg
     5521 gacctgctgg cccggggcgg cttcggcatc ggggacctcc tctgtggggc ccatcctccc
     5581 atggacgccg agagcgcccg gcggctcgtc ctcgccgggg ccgagcccac ccccctccat
     5641 aacaactgct cgggaaagca cgccggcatg ctcctggcct gccgtttgct cgggctgccc
     5701 acaaaggggt acatcgcgcc ggatcaccct ctgcagcggc gggcgctgga tcacaccgcc
     5761 cgcttctgtg gcgtggcgcc ggagtccatc gggttggcca ccgacggttg cagcgtcccc
     5821 acgtaccacc tctccctggc cgccgccgcc cgcggctacg cggccctggc ccaccccgag
     5881 gcggcgggcc tggacgggga ggtggcggcg gcggtccggc gggtggtggc ggcgatgacc
     5941 ggagcgccgg aggtggtggc gggcccgggg cgcttcacca cccggttgat ggaggccacc
     6001 ggcggacggg ttctgggcaa ggagggggcg gaggggtttt atggtgtggc ggtgcgcggc
     6061 ccggtggccc tggggatcgc ggtcaagatc gccgatggtg gcgagcgctg ccgtgacggc
     6121 gtggtgctgg acgtgctgcg ccagctcggc tcgctctcgg gcgccgagtt cgagaccctg
     6181 gcccccttct accggccgcg cctggtcaac tgggccggcc tgcccgccgg cgaggtacgc
     6241 cccgacctgg agctggaaga agcggagctg gaagaagcgg agctggaaga agaagaggtg
     6301 tagacttcgc cggaccgtac agccatgacg agaaccacgg cctccgcgcc cactctgttc
     6361 gggcccacgc tgcggcgggg tctgtgcctg ctcctggccc tcctggccgg cggcgccgcc
     6421 ctggtgaccc tcgccggctg ccgttcctcg cgcggcaagg cccaggagga gggtgggcgg
     6481 cggccgttgt accgcaaggt gagcccgccg gtggcgttcg agatcatccg cgacaccccc
     6541 gagaccctga tcctcgacct gcgcacgccg gaggagtacc tgggggagac agggcatctg
     6601 cgccgggctc tcaacatccc gctcgaccgt cttccctacc gtatcctcga gatcagcgcc
     6661 tggcggcagg agaccgtcct cgtctattgc cgcgccagcg atccgtgcgg tgaggagggg
     6721 atggcggtcc tggtgtccag cggcttcgag gacgccatgc tcatcgacgg cgggatcgac
     6781 ggctggatcg aggagggctt caagaccgag ctgcccgcgg gcctcgccgg ccagggcccg
     6841 gtgcctccgc cgcccccgcc gctcgtccca tgaagaagac cgccggactg ctcgcggcgg
     6901 cgctgctgcc gcttctggcg ttcttcccgg cggcggcgca acggcccgcg gcgctgccgt
     6961 acgccgacgc gatccgcttg gccgaggcgt tccggctggc cgagacggta caggagcggg
     7021 tcttcccggg gtggagccgg gcgcccttct cggtcctcct ggcgagcggc gagcgcgacc
     7081 tgctggtgcg ccatcccgag ccgtccgcgg agttctcgga gctgggctac tcctccatcc
     7141 tgcgcgagcg ggtcttcacc cgcccgggca ggaccgcccc cgacctcctc gccaccttcc
     7201 cggtgatcgg cgcctccccc accgtggtgg tcggcagcgc cgagcggacc ggcttgcgct
     7261 ccaccgagtg ggtgctgacc gtcctccacg agcacttcca tcagttccag atggcggatc
     7321 cgggctactt cgcggcggtg gagcgactcg gactcgcggg cgtcgaccgg accgggatgt
     7381 ggatgctcaa ctatcccttc ccctatgacg cgcccgccac cgccgcccgt ttcgccgacc
     7441 tgtcacggcg gctcgccgcg ttgcttcgcc agggggcgga cgcggcgccg gccaccgtcg
     7501 aagccttctg gcgggactac cgggccttcc tcgccggcct gggggagaag gaccgcctct
     7561 acttctcctt ccagatctgg caggaggggg tgccgcgtta catcgagctg cggtcggcgc
     7621 aggtggcggc cgaggcctac gagccgtcgc tggagttccg ggcgctgccg gacgcccgcg
     7681 gcttcgacga ggtggccgga gggctgcgga cggacatcct tgcccagctc gacagcgcca
     7741 acctggggcg ggaccgccgg gtggccttct atgccttcgg cgccggcctc tgcctgctgc
     7801 tcgaccgctc caaccccgac tggaagtccc gctacatggc ggagaggttc tctctggagc
     7861 gctccgccgg ggtaccctcg ggccgccgag agagccgccg ctagatcccg caggcttgcc
     7921 ccgatgccgc agatcggcta aaaagggaag tctacggaag cttcacgcac caacaccgcg
     7981 gctccgccgc tgcagggaga tgtcatgacc gtccatgctc cgatttccac cgccgagtcc
     8041 ctccgggagc tcaaggaggc actgggcgac cgcatactgc tggacgagga ggtgcgggcc
     8101 cagttccgct ccgacttcgg ccgctatgtc gaccggctgc cgggcgccgt cgcccgctgc
     8161 atgtcgcctg aagaggtcgc gcaggtgatc cgcttctgtg ccgagagggg catccccgtg
     8221 gtggcgcgcg gccagggtca cactcagacc ggccaggcga ccaccgaggg gggcgtcctc
     8281 ctcgacacct cgtcgatgca gacgatccac gagatcgacg aggagaacga gaccgccacg
     8341 gtggacggcg gcgtggtctg gcgcagcctg gtgcgcgcca ccgtgccgaa ggggctggtg
     8401 ccgcgggtgc tcaccaacaa cctgggggtg gccatcgccg gcaccctgtc catggccggc
     8461 ctgggcgtcg ccagcttccg ctatggcacg caggcggaca acgcggtcga gctggtggtg
     8521 gtcaccggca ccggcgacat cgtcacctgc tcgcgggagc agaacagcga ccttttcgac
     8581 gtggcccgtt gcggcctcgg gcagttcggc gtcatcgtcc gcgccaaggt gcggctgcgg
     8641 cgctgccagt cgatggtccg caagtactac ctgctctatg acgacctcgg ggtgctgatg
     8701 gaggagtcca agaggatcat ggacccgtcc aacgcgacct tctcgtcgct cgagtcgtgg
     8761 tgcaccccct gcttccaggg gatcaagaag atcggcgagg ggatggagct ggccgaaggc
     8821 gtccagacct tcgcctactg gatgtatccg ctccacctga cggtggagtt cgagccgggc
     8881 caggagccgg acgatcaggc ggtgctcgcg ggcctcaccc cctaccgtca cctcgagacc
     8941 tccgacttca cccagctcga gttctgcgag cggatggacc cggtgttcga gctgtggcgc
     9001 cgcggcggct actgggacat ggcccatccc tggatggaga ccaccctgcc gtgggacacg
     9061 gcgcgcgagt tcatcgagag cgccctggcc atcacgccgc cgcaggccct gggaccgggc
     9121 ggccacatcc tgctctggcc ggcctacacc cggacctccg aggtgccact cttcatgcac
     9181 ccggaggggg agtacgtcat gggttggggc atcctgcccg gcgtgccgtt caagttcctc
     9241 gaccgggcgc tggcgcagct cgacatggcc agcgaccttt ccatcgccta cggcgggaag
     9301 cgctacctgt ccggctacat caccttcaac acggcggaga agtgggcggc ccacttcggc
     9361 gaccgctggc cgatgatcct cgcggcgaag aagaagttcg atccggccgg gatcatggcg
     9421 ccgggcttca tccagtacga gtgagcccgg ctccggcagg gtgaaatgga aagggcggcc
     9481 gcgaggccgc cctttcgtcg tctgagggag gggggcggtt taccgccagt caccgtagcc
     9541 gtaccggtcg tcccggtagt cgtggcggtc caagtactgc tcgaacgtga tgcggtcccg
     9601 ctggaagtcg tacgaggcgc tctcgtgccg gttggtgtcc cacagggtct tcacctggat
     9661 cacctcgaag ccggtgccgc gccagtcgca gacgccgccg tccaccaccc ggccctggag
     9721 ccgcatgtgg tcaccctgct ggacgtcgcc gatgttgccc accagggcga acatctcgcc
     9781 ggtctggtga tccttgaaca ggctgcagcc ggagccctcg tcgacgacga agccgtcgat
     9841 gtggaaccgc tcgttcgccc cgtagtagcg gccgtcataa cggtagcggc cgccgttgcg
     9901 ctggtagtct cgatagtagc ggttgtcgtt gtagcggttg ttgttgtaga cctgccggtc
     9961 ccgcgggtag tagttgcggt tgtcccgccg gacctgggcc tgacggcgca gctcggcttg
    10021 gcgcctcaac tcggcctgac gctggatctc ggcctgccgc tggagctgga cctgccgctg
    10081 gtactgctgg cgctggagct gggcctggcg ctgctgctgg gcctgcagct tgatctggtt
    10141 ctggcgctgc tgctggcgca ggcgggcttc gcgctgctgc tgggcctgga gcttcacctg
    10201 gttctttctc tgctgctgct ggagccgctc ggcgtcgcgc cgctgctgcg cctgggcctg
    10261 gacctggcgg cgctgttggc cgcggttctg cttggcctgc tcccgctgct gcttggcttg
    10321 gctacgctgc tgctgagccc gaccccggcc ctgagcctgg ttctggcgct tgtttctctc
    10381 gttcttcttc tcgcccctct ccatctggac ggcggcgaag gcggcggacg gcgttgcgag
    10441 gagctcgacc ggcaggacaa gggtcgtcgt cagcaggacc ggcccgaaga tcttcgtgag
    10501 cgatcggtgt cgtaggttca tggtgtgcct ccttgtggcc ggcgtctcgc ggcagccgct
    10561 cgacgctctt ggcttccata gaaagcaaag gccttgccaa tgacgtaagt gtcctggtat
    10621 cagcgtttaa ctgcttattc ccgttttgcg atggaggttt tgtcctctcc tggtgacggc
    10681 tccggccccg ggtgtctcgg cgaggctccg gctcctctga tacgctcccg ccatgaccga
    10741 agctctctcg actccgacga cgactatccg catcgcccac agcccggact cggacgatgc
    10801 cttcatgttc tacgccctga cccagggtct gctcgacacc gaggggctcg ccatcgagca
    10861 tgtcctgcgg gacatcgaga ccctgaacca ggcggccttt cagggaacgt acgagatcac
    10921 cgccctctcg ttccacggct acgcgcatct ggcggaccgg taccagctca tgccctcggg
    10981 ggcgagcttc ggggacggct atggcccggt ggtggtgtcc cgcgaacggc tggggcgggc
    11041 ggacctggtg ggttgcaagg tcgcgatccc cgggcagctc accacggcgc acctcgccct
    11101 ccgcctctgg cagccggagg tggaggtcca gatcgtcccc ttcgatcaga tcctggacgt
    11161 ggtgcaggcg ggggatgccg aagccggcgt ggtgatccac gaggggcagc tcacctatca
    11221 ggagatgggg ctgcgctccg tcgtcgacct gggagcctgg tggaaggagg agacgggcct
    11281 gccgctgccc ctgggtggca acggcatccg ccgcgacatc gatccggagc tcaagctccg
    11341 gctctgccgg ctcctcaccc agagcatcga gtacggcctc gacaaccggc aggaggcgct
    11401 gggctacgcg atccgctatg cccgcgggct ggaggacgac ctggagcgct cggaccgctt
    11461 cgtcggaatg tacgtcaacg actggacgcg gggttacggc gaggccggcc ggcgcgccgt
    11521 gcagctcctg ctggaccggg gccacgaggc cggggtgctg ccccggcgga tcgaggccga
    11581 gttcatcgag atctgagccg cctgatgctg cgtccggccg ctctcgtctt cgatctcgac
    11641 ggcaccctga tcgactcccg gcgggacatc accaccgcca tcaaccgcat gcgggcggac
    11701 ctggggcgtc cgcccctcac cctggaacag gtggtgacca tggtggggga gggggcgaag
    11761 ctcctggtct tccgggcgct gggcggcgac gtgccggagc agatcctgga gagggcgctg
    11821 tccacctacc tcgagcacta ccgggacgtc tgcctggaga ccacccagcc ctatcctggg
    11881 gtcgaagccc tgttggagtc cgtggccgcc cggtatccta tggcgctgct cagcaacaag
    11941 ggggaggcgc tctcccgtaa gatcctggac ggcctcaatc tttcccgctt cttccgcgag
    12001 atcctgggag gggacagcct gccgacccgg aagcccgacc ccggcgggct gctccacctg
    12061 gcgcggcgcc tgcaggtccc gccggagcgc ctgctgatgg tgggggatac ctgggtggac
    12121 gcggtgaccg cccgaaacgc cggctgcccc ttcggcctgg tggagtgggg cttccctcgg
    12181 acgccggcgc cggaggagct ccaggccgac ctgcgcaccg cggaccctga cgagctcagg
    12241 gtccgtctcc tgagcctgag cggccaggca cccgagccga aaccgtcctg acgtctgcgc
    12301 gggccagttt ggatcgtatg gccgacaccc tctctccctc cttgccccta tccgagcccc
    12361 ggcgggtccg ggtgggcatc tcggcctgtt tgctgggtga ggaggtgcgc tacaacggcg
    12421 gccacaagcg ggactttctc ctcctcgacg ccctcggccg ctttgtcgaa tgggtgccgg
    12481 tctgtcccga agtggagatc gggctggggg tgccgcgccc gcccatccgt ctggcccgga
    12541 tcgccgaggg agacgacggg gtgcggctgg tgatgccagg ccgcggcgag gacctcacgg
    12601 acctcatggt ctcctacgcc gagcggcggg tggatggctt ggcccggctc ggccttgccg
    12661 gctacgtgct caagaaggat tcgccgagct gcggcatgga gagggtcacg gtctggccgg
    12721 ccggcggcgc gccggccgag gaggggcggg gcctcttcgc cgaggttctg atgcggcagc
    12781 tacccgatct gccggtggag gaggaggggc ggctcaggga tcccatggag cgggatcact
    12841 ttctcagccg gatctatgct tacgaccgcc ggctgagggc gaacgaggat cgacccaccg
    12901 cccgccagcc ctccacccca tgagctgatg ctgctcaatc acgcctgagg gaacggggtc
    12961 ccgcgccttg cggcgccgcc gccgatgcga gatcatcgat agactcaagg aggtacttcc
    13021 gtgaagaagc ttatcaggac atccgttctc gctccggccc tggccgcaat catcgccgtt
    13081 ctcagcgcgg gggccgcccc gtgcctcgcc ggcatccact acaaggccgt cacccgcaac
    13141 gagggcggac aggagaagac ccccgacttc caggtcgagg gctgggtctc gggcgacaag
    13201 gcgcgcgtcg aattcatcgg ggccaatccc tccggcgcca agggggccta cctgatcacc
    13261 cgcgacggcg gcaaggtcgt ctacctggtc aatcccgagg aaaagagcta cgccgagtgg
    13321 gacatgcagg ccatgctcgg gctcgccggc agtatcatgc aaggcatggg gcccctgctc
    13381 aagttcgagg tctccgaccc caaggtggag aagctcgcgg aggaggacgg cggctcgatc
    13441 ctgggcgtcc ccacgcggca cgtccgctac cgcaccagct acaccatgaa ggtcaaggtg
    13501 ctcggcatgg gcaacgtctc cgacgtcgtt tccgagcagg acatctggac ctccgaccgg
    13561 ctgcaggatc gggcgctgag cgtctggctg cgctccgagc cgccgcgctc cggcaacgag
    13621 cagctcgaca agctgatcgc gtcgcagtac aaggtccagg gcttcccgct gaagatggtc
    13681 accgtgacca ccagcacccc ccagaagaag gggcagccga ccgtcacccg cagcaccatg
    13741 gaggtgaccc agctcgacac ctccgccacg gtgccggact cccggttcga gatcccggcc
    13801 ggctaccagg aggcgcagat ggtgccggcg ggtgagggcg acgaggcgga gcagggaggc
    13861 cccctcggcg ggctgctccg ccggaagaag agcaacagtg gtgggaactg aggagagtgc
    13921 catgagatcg agagcgctga tgctggccgt cgccgcggcg gtcctcctcc ctgtcctggc
    13981 ctgggctgat agcccggcca agggggagac ctacggcaag ggagtgagcc tgacccagac
    14041 cacctccatc caggagattc tcgcccggcc cgacgatttc gtcggcaaga cggtgcggat
    14101 cgaggggccg gtgctggacg tctgcccgat gaaggggtgc tggatggagc tcggagccca
    14161 gcaggcggac ggtacggcca gtctgcgggt caaggtggag gacggcgtga tcgccttccc
    14221 ggtgaccgcc aaggggaagc tcgccgtggc cgaggggacg gtggaggcca tccccatgac
    14281 gcgcgagaag tatgtggact ggctcgccca tctctcggag gagcggggag agaagctcga
    14341 cccctcgacc gtcggggagg gaccgttccg gatcctccag atccagggga ccggcgccag
    14401 gatcgagggc ccgtgagcga gcgggaggac gaggccggcg gcgcgcgcca tctccccggc
    14461 ggcgagcgtc tggggagccg cgagatcttc cgtggccgta ccgtccatct ggacgtcgac
    14521 catgtgcgcc tgcccaacgg caacgagatg gatctggagc tgatccacca tcccggggcc
    14581 gcagccgtcg tgccggtgct cgagggggac gtgctgatgg tgcgccagta ccggtacgcc
    14641 accggcggct ggctcctcga ggtcccggca ggcaagctcg acccggggga gtcgccgaag
    14701 agctgcgcct tccgcgaggt ggaggaggag accggctacc ggcccacgga cctccagccg
    14761 ctgggctgga tctggacgag ccccggcttc accgacgaga agatctggct ctacctcgcc
    14821 accggcctca ccgccacccg ccagcaactg ggcaaggacg aggtgctctc cctggagcgc
    14881 ctgccgctcc gggaggccat cgacaaggcg gcccgtggcg agatccatga cgccaagtcg
    14941 gtctgcgccc tgctgcgtgc cgcccggcac ctcgaccatc agtagcttcc tggcacgagc
    15001 gctgcatctc cccccgcgct gtttcggcac tttgcgagag gagatgcatg cgatcagcca
    15061 tcctgactga acctcgccgt ttcgacatcc agtccatgga gccgccacag cccggagagg
    15121 gcgaggtgcg gctgcgcgtc ctgggctgcg gcgtctgcgg ctccgacatg gggccctgga
    15181 aggggctcca cggtcttacc tatcccatgc cggcgggcgc gccgggtcac gaggtcttcg
    15241 gcaccgtcga atccttaggt ccgggcgtcc agggcttaca agtgggaatg ccggtgacgg
    15301 ccctcaccta tcgggcctat tccgagtacg acatcgccaa agccgactcc ctggtccccc
    15361 tgccgccctc cctggccggc cggccggtcc tgggcgagcc cgtggcctgc gccgtcaacg
    15421 tcgtccggcg ctccggcgtg cgcgaggggg ataccgtcgt gctcctgggc accggcttcc
    15481 tcggcgccct cgtcctccag ctcctgcgcc gcgagcgtcc gggccgcgtg ttcgccgtgt
    15541 cccgtcgtcc tttgtcgccc ggcctcgccg agcgcttcgg agccgacgag gtcctgacct
    15601 atgacgacga cgtgcgcggg cgggtcggcg cggccacggg cgggcagatg gccgatgtag
    15661 tcatcgaggc cacgggctac cagatgccgc tcgacctcgg cgccgagctg acgcggatac
    15721 gcggccggct actgatcgcc ggttatcacc aggagggccc gcggacggtc aacatgcagc
    15781 tctggaactg gcgcgggctc gacgtcatca acgcccacga gcgcgatccg gagatctacc
    15841 gcagcggcat ggaggagggg gtccgccggc tggccgcggg ggagatcgac ctcgcgcccc
    15901 tcatcaccca ctcgttcccg ctggccgaca tcaaccaggc gttccgcacg gcggaggagc
    15961 ggcccgccgg gttcctgaag tcgctggtct tcacgggagc ttcggcgtga cccctcgtct
    16021 cggcttcgcc ggcctcggct ggatcggggc catgcggctc gacgcggtgg ccgcggtcgg
    16081 ggcgggtgag atcgcggccc tctgcgagcc caccgaggcc cgcctggagg aggtcgcccg
    16141 ggcccacccc ggcgccgccg ccttcacctc gttggaggag atgctgtccc gctccggcga
    16201 cctcggactc gacggcgtgg tgatcgcgac gcccaactcc ctccacgctc cccagaccct
    16261 ggcggccctg gaccgcggcc tcgccgtgtt ctgccagaaa ccgctggcgc tctccggcca
    16321 ggaggcgcgc gagatggtgg acgccgcccg ccgggccgac cgcctgctgg gcatcgacta
    16381 ctcctaccgc ttcaccgagg ggatgaaggc gcttcgcggc atggtggcgc ggggcgagct
    16441 cggccgggtg ttcagcgtcg acaccgtgtt ccacaacgct tacggcccgg acaagtcctg
    16501 gtgcagggac ccgcgcctcg ccggcggcgg cgcgctcatg gatctgggcg tccacctcgt
    16561 cgacctcgcc ctatgggtat tggacgacgt agcggtcgag gcggtccacg gcggggtgtg
    16621 gagcggcggc gaggccatcc acggcctcgg gatcgacgac ttcgccagcg cccggctcga
    16681 gctggcgggg ggcgcggcgg tctccctggc ggtgagctgg aacgcccacg ccggccagga
    16741 ctgcgtcatc cgcctggccg ctttcggcac ctccgggggg gccgagttcc gcaacgtcgg
    16801 ggggagcttc ttcgacttcg agctggtccg cttccacggg cgctcgggcg aggtggtcgt
    16861 ccgcgagtcc cgggactggt tgggcaaggc gatcctcgac tggaccggcc gcctgggcca
    16921 gggaggacgc ttcgaccccg agatcgagcg cagcgtcacg gtcagccgcg ccgtcgacgc
    16981 cctctacggc agggattgaa aggaaaaggc ccggggttgg cggccccggg cctttcgtgg
    17041 tgtccagggg agggggctag gccacgtccc gcacgtacag gtactcctgg atgccgtgcc
    17101 ggaggaggaa ggaccggtcg acgtgaaagc ccttgagccg gtcattgacc tccgccgggg
    17161 cgtagcgcgg ccccggacgc tccagggccg accgccgctc gtacgagagc gtctgctcgt
    17221 cgacgatctt gaagcggatc ggctgcgccg ggatctgctt ctggttcgac atcagggcga
    17281 acag
//
LOCUS       DASPJI010000826         3973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_224480, whole genome shotgun sequence.
ACCESSION   DASPJI010000826 DASPJI010000000
VERSION     DASPJI010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224480"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1028)
                     /locus_tag="VEL74_23340"
     CDS             complement(201..1028)
                     /locus_tag="VEL74_23340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012946988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HYG65536.1"
                     /translation="MNPFHDAVAVVTGGASGLGRAFCEELARRGARVVVTDIGEEGAR
                     AVAAAIVAAGGQATAARLDVTRAEDVQRLLEETSATHGRLDYLFNNAGLAVSGEVRDL
                     SLEHWRRIIDVNLWGVIHGATAAYALMTRQGSGHIVNVASLAGLTGFPTATPYATTKF
                     GVVGLSLSLRAEGEGLGVKVTAVCPGFVKTSIFDAGTYVRSRKEDVLASIPFKMIGAA
                     DAARQTLDGVARNRAVIVYPFYGRVMWWMTRLSYSLAALLHAQTVGRFRAKRGKLPV"
     gene            1121..>3973
                     /locus_tag="VEL74_23345"
     CDS             1121..>3973
                     /locus_tag="VEL74_23345"
                     /inference="COORDINATES: protein motif:HMM:NF037694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HYG65537.1"
                     /translation="MPILVSFRDKVMPRLAAPFRRQWQWKDLVIALLLLLVGSLSAYA
                     FRAPIAAAAARLRADAQGAEMVSVFDVAVDRQGRQYVDFIFDRPLGKDKVGTILDPAP
                     AEIEPALGGSWKWQDTNALRFQPSGGFPVASEFTFTLIPDRILTEGLVFTGETEHVVR
                     TDQFLVENVEITEEPALDGKAKIFFRGQIQFNYPVDPKVLIPKLRLEDPNLPEPVPVV
                     LETDWNSAVIAFRTEPVQKERDERTLRLVVSGDLTPIGGNVPLGEDFVREIPVGSSTK
                     LSVWSITAEPGPRESTLRVRFSSPISAAVAEKYLKIEPAVRVRLSAERNELAVTGELL
                     PGNTYDVTIGRGMPATDDAVLGEDYNQEVAIPDLDPSVGFQSQGMFLAASGRHTVAVE
                     SVNVPKVRMTIDRVYLNNLFFLFQYGGFFDSETGYFGELPHALGDRLKDETLEPGGAR
                     NQRRVTPITLDRWVDVKEPGLYRVSVGQPDDWEASQRWLLLTDLGAVAKRGRGELMVW
                     VSSLTDLGAIDGAQVALYTDQNQLIGEGTTDGSGLWRIEDAKALASGAKGRPYMVTIE
                     KGDDFTFLLLDQMGIDVTGLDTGGAEPIGEGYTAFLYGERDLYRPGETVEGLAVVRDG
                     TLSPSPAMPAVLRHLDPQGREIETQRVQIDDRGLGELELALPAYSLTGHHTLELVVAE
                     KVIGQYRFQVEEFVPDRISVAIVTPKEKVGPGQDLSYQVQSNYLFGPPAGGLPVETRV
                     RLVDADFAPKGFENFTFRNPERKLEEREILSQQGKLDTEGRSPFTVTMPAGAPVPSSL
                     EAVITARVQEQGGRGVAAMERLRVDPYPYYIGLRRLGEGYPEPGKPVEFEFVGVDPSG
                     KPVAAKALRADLFLDRWNTVLRRTSGGGYRYETTRDPRLIDSQAVAGGKERGKLSFTP
                     REYGSYRAVITDPETQASAEVEFFAAGWGYSPWAIKN"
BASE COUNT          594 a         1437 c         1371 g          571 t
ORIGIN      
        1 tgccccacgg ctcgccgggc cggggcgagc cggacgcggc cccccacggt ccccgcaaag
       61 ggcccacggc gtgggtcttc tccagctcgt ccgtgctcaa cggcggtccc tccacccccg
      121 gaacggccgg gggcgcctgc ggcgcgatgt ctcaggtctc gaaggagaag tgtagcggag
      181 gtcgggctgg aaggccccgc tcagaccggg agcttgccgc gcttggcccg gaagcgcccg
      241 acggtctggg cgtggaggag cgcggccagg ctgtagctca ggcgggtcat ccaccacatg
      301 acgcggccgt agaaggggta gacgatgacc gcccggttgc gggcgacgcc gtccagggtc
      361 tggcgggcgg cgtcggcggc gccgatcatc ttgaagggga tgctggcgag cacgtcctcc
      421 ttgcggctgc ggacgtaggt gccggcgtcg aagatggagg tcttcacgaa gccggggcag
      481 acggcggtca ccttgacccc cagcccctcc ccctccgccc gtagcgagag cgacagcccc
      541 acgacgccga acttggtggt ggcgtacggg gtggcggtgg gaaacccggt gaggccggcg
      601 agcgaggcga cgttgacgat gtgccccgat ccctggcggg tcatcagggc gtacgcggcg
      661 gtggcgccgt ggatcacccc ccagaggttg acgtcaatga tcctgcgcca gtgctcgagg
      721 gagaggtcgc ggacttcgcc ggacaccgcc aagccggcgt tgttgaagag gtagtcgagc
      781 cgcccgtggg tggccgaggt ctcctccagc aggcgctgga cgtcctcggc ccgcgtcacg
      841 tccagccgcg ccgccgtcgc ctgtcccccg gccgccacga tggccgccgc caccgcccgc
      901 gccccctcct cgccgatgtc cgtgaccacc acccgcgccc cgcggcgcgc gagctcctcg
      961 cagaaggcgc gccccagccc ggaggcgcca ccggtcacca ccgcgaccgc gtcgtggaag
     1021 ggattcatgg gtagggcgag tgtaccccga aatcttcccg cgacgccggc caccttttgg
     1081 tgtagcgtag agctgctttc cgcggagaca tcggaaccct atgccaatcc tcgtcagctt
     1141 ccgggacaag gtcatgcctc gtctagccgc ccctttccgc cgccagtggc agtggaagga
     1201 cctggtcatc gcccttctcc tcctgctggt gggcagcctg tcggcctatg ccttccgggc
     1261 gcccatcgcc gccgccgcgg cgcgcctgcg ggcggacgcc cagggcgccg agatggtgtc
     1321 ggtgttcgac gtcgccgtcg accgccaggg gcgccagtac gtcgacttca tcttcgaccg
     1381 gccgctgggc aaggacaagg tcgggacgat cctcgacccg gcgccggccg agatcgagcc
     1441 ggccctcggc ggctcctgga agtggcagga cacgaacgcg ctgcgcttcc agccgagcgg
     1501 tggctttccc gtcgccagcg agttcacctt caccctgatc ccggaccgca tcctcacgga
     1561 ggggctcgtc ttcaccggcg agaccgagca cgtggtccgc accgaccagt tcctggtgga
     1621 gaacgtcgag atcaccgagg agccggcgct cgacggcaag gccaagatct tcttccgcgg
     1681 ccagatccag ttcaactacc ccgtcgaccc caaggtcctg atccccaagc tgcggctgga
     1741 ggaccccaac ctgcccgagc cggtgccggt cgttctggag accgactgga acagcgcggt
     1801 catcgccttc cgcaccgagc cggtgcagaa ggagcgcgac gagcgcacgc tgcggctggt
     1861 ggtctctggc gatctcaccc cgatcggcgg caacgtgcca ctgggcgagg acttcgtgcg
     1921 cgagatcccg gttggctcca gcaccaagct gtcggtgtgg agcatcacag ccgagcccgg
     1981 gccgcgcgag tccaccctgc gcgtgcggtt ctcgtcgccg atctccgccg ccgtcgccga
     2041 gaagtacctg aagatcgagc cggcggtgcg cgtccgcctc tccgccgagc gcaacgagct
     2101 cgccgtcacc ggggagctcc tgcccggcaa cacctacgac gtcaccatcg gcagggggat
     2161 gcccgcgacg gacgacgccg tcctggggga ggactacaac caggaggtgg cgatcccgga
     2221 cctcgatccg tccgtgggct tccagagcca ggggatgttc ctcgccgcct ccggccgcca
     2281 caccgtggcc gtcgagagcg tcaacgtccc caaggtgcgg atgaccatcg accgggtcta
     2341 tctcaacaac ctcttcttcc tcttccagta cggcggcttc ttcgactcgg agaccggcta
     2401 cttcggcgag cttccccacg cgctcggcga ccgcttgaag gacgagacgc tggagccggg
     2461 cggcgcccgc aaccagcggc gggtcacccc gatcaccctc gaccgctggg tggacgtcaa
     2521 ggagcccggc ctctaccggg tctcggtcgg ccagcccgac gactgggagg cgagccagcg
     2581 ctggctgctg ctgaccgacc tcggcgccgt ggccaagcgc ggccgcggcg agctcatggt
     2641 ctgggtgtcg tcgctgaccg acctcggcgc catcgacggc gcccaggtcg ccctgtacac
     2701 cgaccagaat cagctcatcg gcgaggggac caccgacggc tccggcctct ggcgcatcga
     2761 ggacgccaag gcgctggcct ccggcgccaa gggccggccc tacatggtga ccatcgagaa
     2821 gggggacgac ttcaccttcc tcctcctcga ccagatgggg atcgacgtca ccggcctcga
     2881 caccggcggc gccgagccga tcggcgaggg ctacaccgcc ttcctctacg gcgagcgcga
     2941 cctctaccgg ccgggcgaga cggtcgaggg tctggccgtg gtgcgcgacg gcactctgtc
     3001 cccgtcgccg gcgatgcccg cggtgctccg ccacctcgac ccccagggcc gggagatcga
     3061 gacgcagcgg gtgcagatcg atgaccgcgg cctcggcgag ctcgagctcg ccctgccggc
     3121 ctattcgctg accggccacc acaccctgga gctggtcgtc gccgagaagg tcatcggcca
     3181 gtaccggttc caggtggagg agttcgtccc cgatcgcatc tcggtcgcca tcgtcacccc
     3241 caaggagaag gtgggcccgg gccaggacct ctcctaccag gtgcagtcca actacctgtt
     3301 cggaccgccg gccggtggcc tgccggtcga gacccgggtg cggctggtgg acgccgactt
     3361 cgcccccaag gggttcgaga atttcacgtt ccgcaacccc gagcgcaagc tggaggagcg
     3421 cgagatcctc tcgcagcagg gcaagctcga caccgaaggg cggagcccct tcaccgtcac
     3481 catgccggcc ggcgctccgg tcccgtcgtc gctcgaggcg gtgatcaccg cgcgggtgca
     3541 ggagcagggg ggccggggcg tggccgccat ggagcggctg cgggtcgatc cctatcctta
     3601 ctacataggg ctgcggcggc tgggcgaggg ctaccccgag cccggcaagc cggtggaatt
     3661 cgagttcgtc ggcgtcgacc cgtccggcaa gccggtggcg gccaaggcgc tgcgcgccga
     3721 cctcttcctc gaccgctgga acacggtgct gcgccggacc tcgggcggcg gctaccggta
     3781 cgagacgacg cgcgacccgc ggctgatcga cagccaggcg gtcgccggcg gcaaggagcg
     3841 gggcaagctc agcttcaccc cccgcgagta cggcagctat cgggcggtga tcaccgaccc
     3901 cgagacccag gcgtcggccg aggtggagtt cttcgccgcc ggctggggct actccccgtg
     3961 ggcgatcaag aac
//
LOCUS       DASPJI010000827         5429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_275016, whole genome shotgun sequence.
ACCESSION   DASPJI010000827 DASPJI010000000
VERSION     DASPJI010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275016"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2643)
                     /locus_tag="VEL74_23350"
     CDS             complement(<1..2643)
                     /locus_tag="VEL74_23350"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65538.1"
                     /translation="MNQEELARRIAELPLEKRALLFQQLQKQKAHEEPEQPRIARVNR
                     SPGMFRLSFAQQRLWFLQQYEPDSPEYNIPQGFRLVGPLDVEALRRTLDAIVARHEAL
                     RTTFQAVDGEPMQIIEERLVVDLPVTDLSGSPDAEAEAMEAAAADSRQPFDLRMGPVF
                     RPLLFKVGADEHLFFFNVHHVVYDGWSRGVFLGELRAIYEAFAAGEPSPLPELEVQYL
                     DFGLWQREWLQGDVLEAQLSYWRDRLAGTPHLDLVTDRPRPALRTYNGDTAPVRLPRE
                     TTGALTRLAQREGGTLFVALLAAFKSLLHHYTGQEDLALGTLIANRTRPEIEKLIGLF
                     ANSLALRTDLSGDPSFRELVRRERETALGAYAHQDLPFEKLVDELHPERDLARTPVFQ
                     VLLILLNVPGKALELPGLQIRQVPVDSRTSKFEMTLYMVEGENGLDGYLEYNTDLFDL
                     STIERFLGHFRAVAEGVAADPDRPLSELPWLAGAERRQLLVDWNATAVDVPRDVCLHE
                     LIASQAARTPEVIAADFEGEPLTYGDLEARANRLAHHLRSLGVGPEVLVGLSMERSLD
                     MLIGVLAVLKAGGAYVPLDPEYPRERVAFMLEQSHAPVLLTQERLLAKLPEHSAVLVA
                     VDRDAEAIASRPATPPVSGVGPDNLAYVIYTSGSTGKPKGVQIPHGAVVNFLLSMSKT
                     PGLSAADTLLAVTTLSFDIAGLELYLPLVNGAKVVLVSRDTAQAGEQLVERLTNSGTT
                     VMQATPATWRLLLSAGWQGNPNLKILCGGEALPRDLADQLLDACGSLWNVYGPTEATI
                     WSMAHRVDAGGGANLPIGRPIDNTQIYLLSPRLQPVPAGVPGELHIGGLGLSRGYRNR
                     PDLTAERFIPDAFGPAGAEPGGRLY"
     gene            complement(2718..>5429)
                     /locus_tag="VEL74_23355"
     CDS             complement(2718..>5429)
                     /locus_tag="VEL74_23355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65539.1"
                     /translation="SRGEASPLPPLALQYADFAAWQRSWLQGGVLERQLGYWRRQLDG
                     APRVLELPTDRPRPAVQSAHGARHYFALPVELAQGVSALGRREGTTLFMTLLAGFDVL
                     LSRWSGQEEVLAGWPIAGRDRAGLEGLIGFFANMLVLRADLGGEPGFRQLLVRLREAA
                     LGAYTHGDLPFEQLVEELQPERHLSHNPIFQVFFVLHHVPDAPPDATRLPGLALSFPS
                     IDNRTSRLDLSLSLTEGRDGLAGFLEYNTELFETATIERMAGHLINLLAGAVADPDAA
                     VADLPMLSAEERLQLLEAWNDTRTALPAESTVHALVEAQVARTPGAVALTFEGEDLTY
                     ADLDARANHLADHLRSLGAGPGTLVGVFLDRSANMVAVLLAVLKSGAAYVPLDPTYPQ
                     DRVAYVVEDSGAAILLTEERLRWELPETTAEIVEIDTLDLADDLAAGELPAAGPDDLA
                     YVIYTSGSTGRPKGVEVRHGGVVNFLASMAHQPGLTAADTLVAVTTISFDIAGLELYL
                     PLSVGARILLASRDEAMDGGSLSTLLEGATVLQATPATWRMLLTSGWQGSPGLKALCG
                     GEALPADLARDISSRVGSLWNVYGPTETTIWSATRSVQVDEAFAAAVPVGRPIANTSI
                     HLLDRRFAPVPAGVVGELYIGGDGLARGYLRRPGLTAERFVPDPFAWESGARLYRTGD
                     LARRLPNGEVEFIGRADHQVKVRGFRIELGEIEAAFSDHPAIAQAVVAVLPDATGSNQ
                     LVAYLVQAEGKVASVGNLRAALRESLPEYMIPSHFVTLQALPLTPNGKVDRKALPVPG
                     GERPELERELVAPRTPIEEGVAAIWSEVLGRPRIGVFDNFFELGGHSLMATQVVSRIR
                     ETYRVDVTLPVLFKSPTVAGLAEAVVQKELERADADMLAQLLASFES"
BASE COUNT          877 a         1785 c         2015 g          752 t
ORIGIN      
        1 gtagagtcgg ccgcccggct cggcgcccgc agggccgaag gcatcgggga tgaacctctc
       61 ggcggtcagg tccggccggt tgcggtagcc gcgggagagt cccaggccgc cgatgtgcag
      121 ctccccgggt acgccggccg gcaccggctg gagccgcggc gacaggagat agatctgggt
      181 gttgtcgatc ggcctgccga tggggagatt ggcgccgccg ccggcatcca cccggtgggc
      241 catggaccag atcgtcgcct cggtgggtcc gtagacattc cacagtgagc cgcaggcatc
      301 caggagctgg tcggccaagt cgcgcggcag agcctcgccg ccgcagagaa tcttgaggtt
      361 cggattcccc tgccagccag cgctcaagag gagccgccaa gtggcgggtg tcgcctgcat
      421 caccgtggtc ccggagttgg tcaggcgctc cacgagctgc tcgccggcct gggcggtgtc
      481 gcgggacacc agcaccacct tggcgccgtt gaccaggggg agatagagct ccagcccggc
      541 gatgtcgaag gagagggtgg tgacggcgag cagggtgtcc gccgccgaca ggcccggcgt
      601 cttgctcatc gagagcagga agttgaccac cgcgccgtgc gggatctgga cccccttggg
      661 cttgcccgtc gatcccgagg tatagatgac gtaggcgagg ttgtccggcc ccacccccga
      721 gaccggcggc gtggccggcc gggaggcgat cgcctcggcg tcgcggtcga cggccaccaa
      781 gacggcgctg tgctcaggga gcttggcgag cagccgctcc tgggtgagga ggaccggcgc
      841 gtgcgactgc tccagcatga aggcgacgcg ctcgcgcgga tactcggggt cgagcggcac
      901 ataggcgccg ccggccttga gcaccgccag cacgccgatc agcatgtcga gcgagcgctc
      961 catggaaagg cccaccagca cctccggccc gacgcccagg ctccgcaggt gatgcgccag
     1021 gcggttggcc cgggcctcca ggtcgccgta ggtcagcggc tcgccttcga agtcggccgc
     1081 gatgacctcc ggggtacgcg ctgcctggga ggcgatcagc tcgtggaggc agacgtcccg
     1141 cggcacatcg acggcggtgg cgttccagtc gaccaggagc tggcgccgct cggcgccggc
     1201 gagccagggc agctccgaga ggggccggtc cggatcagcg gcgacgccct cggccaccgc
     1261 ccggaagtgg cccaggaagc gctcgatggt ggagagatcg aagagatcgg tgttgtactc
     1321 gaggtagccg tcgagcccgt tctccccctc gaccatgtag agggtcatct cgaacttcga
     1381 ggttcggctg tccaccggca cttggcggat ctggagaccc ggcagctcga gcgccttgcc
     1441 gggaacattg agcaggatga gcagcacctg gaagaccggg gtgcgggcga ggtcgcgctc
     1501 cggatgcagc tcgtcgacca gcttctcgaa cggcaggtcc tgatgggcat aggcgccgag
     1561 ggccgtctcg cgttcgcggc gcaccagctc gcggaacgat ggatcgcccg agaggtcggt
     1621 gcgcagggcc agggagttgg cgaataagcc gatgagcttc tcgatctccg gccgggtgcg
     1681 gttggcgatc agtgtgccga gggccagatc ctcctggccg gtatagtggt ggagcagcga
     1741 cttgaaggcg gccaagaggg ccacgaagag agtccccccc tcgcgctggg cgagccgggt
     1801 cagggcgccg gtggtctcac gcggcagccg caccggcgcg gtgtcgccgt tgtacgtccg
     1861 cagcgccggc cgggggcgat cggtcaccag gtccaggtgg ggagtgccgg cgaggcggtc
     1921 tcgccaatag gagagctggg cctccagcac gtctccttga agccactcgc gctgccaaag
     1981 gccgaaatcc aggtactgga cttccagctc cggcagcggc gacggctcgc cggcggcgaa
     2041 cgcctcgtag atcgcccgca actcgccgag gaagacgccg cgggaccagc cgtcataaac
     2101 gacgtggtgg acgttgaaaa agaagaggtg ctcgtccgcg cccaccttga agaggagagg
     2161 acggaagacc gggcccatcc gcaggtcgaa gggctggcgc gaatccgcgg cggccgcctc
     2221 catcgcctcg gcctcggcgt cgggggagcc ggagaggtcg gtgaccggca gatcgacaac
     2281 caggcgctcc tcgatgatct gcatcggctc gccgtccacc gcctggaagg tggtacggag
     2341 cgcctcgtgg cgggcgacga tggcatccag ggtgcggcgg agcgcctcga catccagcgg
     2401 gccgacgaga cggaagccct gggggatgtt gtattcgggg ctgtccggct cgtactgctg
     2461 cagaaaccag agccgctgct gcgcgaagga gagccggaac atgcccggcg agcggttgac
     2521 ccgggcgatg cggggctgct ccggctcctc gtgagccttc tgcttctgga gctgctgaaa
     2581 aagcagagct ctcttctcga gaggcagctc cgcgatccgc ctcgccagtt cttcctggtt
     2641 catacgtcct ccctcaaacc aaagcatcag aaacctgcaa gggggcgaat gggaccaccc
     2701 ctccctgcta caacgtgcta cgactcgaag ctcgccagga gctgggcgag catgtccgcg
     2761 tcggcccgct ccagctcctt ctggaccacg gcctcggcga ggccggcgac cgtcgggctc
     2821 ttgaacagaa ccggcagcgt cacgtccacc cggtaagtct cgcggatacg cgagaccacc
     2881 tgagtagcca tgagcgagtg cccgcccagc tcgaagaagt tgtcgaagac gccgatccgg
     2941 ggccgtccca gcacctcgga ccagatcgcc gccaccccct cttcgatagg cgtgcggggc
     3001 gcgacgagct cccgctcgag ctcgggacgc tctcctcctg gaaccggcaa cgctttgcga
     3061 tcgaccttac cgtttggagt gaggggtaaa gcctgaagcg tgacaaaatg tgaaggaatc
     3121 atgtactccg gcagcgattc gcgcaaggcc gcccggagat tgcccacgct ggccaccttc
     3181 ccctccgcct ggaccagata ggccacgagc tggttgctgc ccgtggcgtc cggcaggacc
     3241 gccaccactg cctgggcgat cgcggggtgg tcggagaacg ccgcctcgat ctcccccagc
     3301 tcgatccgga aaccccggac cttcacctgg tggtcggcgc ggccgatgaa ctccacctcg
     3361 ccgttcggca ggcgccgcgc caagtcgccg gtgcgataga ggcgggcgcc gctctcccag
     3421 gcgaacggat cggggacgaa gcgctcggcc gtgaggccag gccggcggag gtagccccgg
     3481 gcgaggccgt cgccaccgat gtacagctcc cccaccactc cggcagggac cggtgcgaac
     3541 cggcggtcca gcaggtggat ggaggtgttg gcgatcggcc ggccgacagg gacggcggcg
     3601 gcgaaggcct cgtccacttg gacggagcgg gtggctgacc agatggtagt ctcggtcggg
     3661 ccgtagacgt tccacaggga gccgacgcgc gaggagatgt cgcgcgcgag gtccgcgggg
     3721 agggcctcgc cgccgcagag ggccttgagg ccagggctcc cctgccagcc cgaggtgagg
     3781 agcatccgcc aggtggcggg cgtcgcctgg aggacggtcg cgccttcgag gagagtggag
     3841 aggctgccac catccatcgc ctcgtcccgg ctggcgagca ggatccgggc gcctaccgag
     3901 aggggcaggt agagctcgag gccggcgata tcgaaggaga tagtggtcac cgcgacgaga
     3961 gtgtcggcgg cggtgaggcc cggctggtgt gccatggagg cgaggaagtt gacgacgccg
     4021 ccgtggcgta cctcgacgcc cttcgggcgg ccggtggagc ccgaggtata gatgacgtag
     4081 gcgaggtcgt cggggccggc cgccgggagc tcccctgcag cgagatcgtc cgcgaggtcc
     4141 agggtgtcga tttctacgat ctcggccgtg gtctccggca gctcccagcg gaggcgctct
     4201 tcggtgagaa ggatggcggc gccgctgtcc tccaccacgt aggcgacgcg gtcctgggga
     4261 taggtcggat cgagggggac gtaggcggcg ccggacttca agacggccag gagcaccgcc
     4321 accatgttcg ctgagcgatc caggaagacc cccaccaggg tgccggggcc agctcccagg
     4381 ctccggaggt gatccgccag atggttcgcc cgggcatcga ggtccgcgta ggtcaggtcc
     4441 tcgccttcga acgtcagggc gacggcgccg ggagtgcgcg cgacctgggc ctccaccaga
     4501 gcgtggaccg ttgactcggc tgggagcgcg gtccgggtgt cgttccaggc ttccaggagc
     4561 tggagacgct cctcggccga gagcatcggc aggtccgcca ccgcggcgtc ggggtcggcc
     4621 acggcgccgg cgagcaggtt gatcaggtgg ccggccatgc gctcgatggt ggccgtctcg
     4681 aagagctcgg tgttgtactc cagaaagccg gcgaggccgt cccgcccctc ggtcagcgag
     4741 agggagaggt cgagccgcga cgtccggttg tcgatggacg ggaagctcag ggcgaggccc
     4801 ggcaggcgcg tggcgtccgg cggcgcgtcc ggcacgtggt gcaggacgaa aaaaacctgg
     4861 aagatcgggt tgtgcgagag gtgccgctcg ggctggagct cctcgacgag ctgctcgaac
     4921 ggcaggtcgc cgtgggtgta ggcccccagc gccgcctcgc gcagccggac caggagctgc
     4981 cggaacccgg gctcgccgcc gaggtcggcg cgcagcacca gcatgttggc gaagaagccg
     5041 ataagcccct cgaggccggc gcggtcgcgg ccggcgatcg gccagccggc cagcacctcc
     5101 tcctgcccgg accagcggga gagcagaacg tcgaaaccgg cgaggagggt catgaagagc
     5161 gtcgtcccct cgcgccggcc gagcgccgag accccctgcg ccagctccac gggcagggcg
     5221 aagtagtggc gggcgccatg ggccgactgc accgccggcc gcggccggtc ggtgggtagc
     5281 tccaggaccc gcggcgcgcc gtcgagctgc cggcgccagt agccgagctg ccgctccagc
     5341 accccgccct gcagccagga acgctgccag gcggcgaagt cggcgtattg gagcgcgagc
     5401 ggcggcagcg gcgacgcctc gccacgcga
//
LOCUS       DASPJI010000828         6143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_52482, whole genome shotgun sequence.
ACCESSION   DASPJI010000828 DASPJI010000000
VERSION     DASPJI010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52482"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1945
                     /locus_tag="VEL74_23360"
     CDS             <1..1945
                     /locus_tag="VEL74_23360"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYG65540.1"
                     /translation="VDEPPQEVTFLVNGREAGKVTLERGFHDYRLVIPAGALVAGDNR
                     FVARYGFTRVPRELTSGMDPDARRLGVLWDTLRFETGVDPRAEVRATGRQLYIPYGVR
                     VDYYTRFPARAALLFERLQARGDEGAALRVTVRTESEEEGSERVSPSPEAAAVRLPEV
                     EQPVRVSLTAVPAERPKEGKEGAVPAASGVVLTRPALGAVRTAAAGRGTVAAAEAAAV
                     ARRPGQRLPNVIFYMVDTLRADHLGCYGYGKPVSPHVDAFAREATLFETAVAQSPWTR
                     PSVASMFTGVGPKTHGVNGRKDTLSPEALTLAEMLRSRGYRTAGFATNGNVAKAFGVA
                     QGFDTYELLPRARQHSRFVHQRAAEWLAEHGDDPFFLYLHTVDPHSPYKPEEPFRARF
                     APNVPADGIGSLPWLARLQTRKIPATPEVIGHLLALYDAEIAQNDASFGALMDLLRER
                     RLWDDTVVVFVSDHGEEFHDHGGWEHGKTLYAEMVQVPLIIRFPGLGEGKRVASVAQH
                     IDLVPTLLTYLGLPLPPHVEGRDLLPSVLADDEAGDGNTGRERAAFSYMEVDGHRGSS
                     AVTDGWHFIDIQAPTAAEKLYQRREDPAQRQNRTGDSPVARGYLRTLLEVHEGQRRQT
                     LRPGEGVLDEELREQLRALGYIQ"
     gene            2024..3706
                     /locus_tag="VEL74_23365"
     CDS             2024..3706
                     /locus_tag="VEL74_23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HYG65541.1"
                     /translation="MALLLKAEHLKRYKDLTRLLFKYGRGDLAQAATRDPLLAEEDLG
                     ATSTTPGQPEQLAADLEALGPTFIKLGQLLSTRSDLLPAPYIDALARLQDNVEPFSFA
                     EVEDIVSTELGVRLSKAFSDFDSVPLAAASLGQVHRAALRDGRLVAVKVQRPGIRQTI
                     LEDLEAFDEIAGFIDRHTEMGRKFAFKDMLEEFRKTLLRELDYRQEALNLSTLRKNLR
                     EYPRLVIPQPIEDYSTARVLTMEYVRGTKITDLSPLARIDLNGQVLAEDLCRAYLDQI
                     LVDGFFHADPHPGNLLVTDDGRLALLDLGMVARIDPQIQERLLKLLLAVIDGRGHEAA
                     QIAIQIGTPLEGHDENRFRREVADLVGSYQSVPRREVQVGKVILSLARLSADSGVRPA
                     PELTMLGRALLCLDESARALDPDFDPNATMRRYTDAVLSRHMFKRLSPESFVSSTLEM
                     YEFVQQFPSRLNSVLETLAGNRLELKVNAFDETRLMTNLQKIANRIAMGLVLAALIVG
                     AALMMQVRTKFTLFGYPGIAMLLFLMAAACGFVLVLSILFSDDWGISRKRQH"
     gene            complement(3711..>6143)
                     /locus_tag="VEL74_23370"
     CDS             complement(3711..>6143)
                     /locus_tag="VEL74_23370"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYG65542.1"
                     /translation="TFPGLTQLLPSPDRFGEVNLYKLPMWPPEDELRPRQELLERVRE
                     VQKALAPADERFFLIAGVNQKTVTGLVRRPDGGFSYEFSPDGDGVVPVDLALLPGART
                     WYVEDSHGTLPTNPRVAHAIAEILGLGTTNALPGSPPIVVQSEPPQLCLEEDLRKNPY
                     RSQRDGALSQRELRHLLDEVVAPQPRESGLAQTPSQAADGLPLEPSQFPGNRIVINQT
                     RQHRLDLRLALGSITEADTRAIALGIFRDVAPSGPARALDERLGGTITELFRRRMFSG
                     EVGEIFLMPTGKHPLAADHIAFVGLGSFDRFNEEVLQTAAENVIRTFINTRVEEFSTV
                     LFGVGSGERASSVLHSLLTGFLRGLLDADRNHFFHRVVICEKDRERYAAARQEFLRLS
                     TSGLCQGVQISMDEVELPSPPLPPVSRSGPERKDPVYLIVRREGERDPRDGGPGFEVR
                     SALLTAGSQAMVVSGTRPVSEVEIQACVERLKAKEMKMDLTEPGTELADLILAEDVRT
                     VLLRHRDRHLVVVHDAFLSRMPWEILALHADRQSSPGQITFPGGPDIWFPAAEEGMSR
                     RYAADNLSVAKWLEQRIHDGILNILLVLNPTEDLEGAEAEGAALQRLLGRLKGLQMDI
                     IRGSEATRPALLKAFSSGKYDVIHYAGHAEFDPASPEHSGLLCHNEARLTGADLVGVG
                     NLPTLVFFNACESARVRRGSRNTAPPRARGLRPRDLRVRITEAVSFAEAFLRGGIANF
                     IGTYWPVGDREAEIFSLAFYEQILRGRPLGQALQVGREAIRKAQGTKDWANYILYGNP
                     DFVLKEGGTQGM"
BASE COUNT          930 a         2102 c         2148 g          963 t
ORIGIN      
        1 cgtcgacgag ccgccgcagg aggtgacctt cctggtcaac ggccgcgagg cggggaaggt
       61 gacgctcgag cgcggcttcc acgactatcg gctggtgatc ccggccggcg ccctggtggc
      121 cggggacaac cggttcgtcg cgcgctacgg cttcacccgg gtgccgcgcg agctgacctc
      181 cggcatggac ccggacgcgc gccgtctggg cgtgctctgg gacaccctcc gcttcgagac
      241 cggcgtcgat ccccgggccg aggtgcgcgc caccggccgc cagctctaca taccctatgg
      301 ggtacgggtg gattactaca cccgcttccc ggcccgggcg gcgctcctct tcgagcgcct
      361 gcaggcgcgg ggggacgagg gggccgcgct gcgggtgacg gtccggaccg aaagcgagga
      421 ggagggctcc gagcgggtct cgccaagccc ggaggcggcg gcggtccggc tgcccgaggt
      481 ggagcagccg gtgcgcgtct cgctcaccgc cgtcccggcc gagcgcccga aagaggggaa
      541 ggagggagcg gtgcctgccg cgagcggcgt tgtcctcacc cggcctgccc tgggagccgt
      601 gaggacggcg gccgcgggcc gggggaccgt cgcggcggcc gaggctgcgg cggtggcccg
      661 ccgcccgggg cagcgcctgc ccaacgtcat cttctatatg gtggacaccc tgcgggccga
      721 ccacctgggc tgctacggct acggcaagcc ggtgtccccc cacgtggacg ccttcgcccg
      781 cgaggcgacc ctgttcgaga ccgccgtggc ccagtccccc tggacccggc cctcggtggc
      841 ttccatgttc accggcgtcg ggcccaagac ccacggcgtc aacggccgca aggacaccct
      901 gtcgccggag gccctcaccc tggcggagat gctgcgcagt cgcggctacc gcaccgccgg
      961 gttcgccacc aacggcaacg tggccaaggc cttcggcgtc gcccagggct tcgacaccta
     1021 cgagctgctg ccgcgtgccc ggcagcactc ccggttcgtc caccaacggg cagcggagtg
     1081 gctggcggag cacggcgacg acccgttctt cctctatctc cacaccgtgg acccccactc
     1141 gccctacaag cccgaggagc ccttccgggc gcgcttcgct cccaacgtgc cggcggacgg
     1201 catcggctcg ctgccctggc tggcccggct ccagacccgg aagatcccgg cgacccccga
     1261 ggtcatcggc catctcctcg ccctgtacga cgccgagatc gcccagaacg atgccagctt
     1321 tggcgctctg atggatctcc tgcgtgagag gaggctctgg gacgacaccg tcgtggtctt
     1381 cgtctcggat cacggcgagg agttccacga ccacggcggc tgggagcacg gcaagaccct
     1441 ctatgccgag atggtgcagg tgccgctgat catccgcttc ccggggctgg gggaggggaa
     1501 gcgggtggcc tccgtcgccc agcacatcga cctggtgccg accctcctga cctatctcgg
     1561 gctgccactg cctccccatg tcgagggacg cgacctcctg ccgtcggtcc tggccgacga
     1621 tgaggccggg gatggaaaca ccggcagaga gcgggcggct ttttcgtaca tggaagtgga
     1681 cggccaccgg ggctcgtccg ccgtcaccga cgggtggcat ttcatcgaca tacaggctcc
     1741 caccgccgcg gagaagctct accagcggcg cgaggatccg gcgcagcgcc agaaccggac
     1801 cggcgacagc ccggtggcgc gcggttacct ccgtaccctc ctggaggtcc acgaggggca
     1861 gcggcggcag accctgcgcc ccggcgaagg ggtgctggac gaggagctcc gcgagcagct
     1921 cagggctctg ggctacattc aataggcgca gtccggcggt ggcattccct gtgcattagg
     1981 ggaggccgac agcgctcgtt ggtgtagttt cgatggagga agcatggccc tgttgctgaa
     2041 ggcggagcat ctcaagcgat acaaggacct cacccgtctg ctgttcaagt acggccgggg
     2101 cgacctggca caggcggcga cccgggaccc gctgctggcg gaggaggatc tcggcgccac
     2161 ctccaccacg cccggccagc cggagcagct ggccgccgac ctcgaggcgc tgggccccac
     2221 cttcatcaag ctggggcagc tcctctccac ccgctccgac ctgctgccgg ctccctacat
     2281 cgacgccctg gcgcgcctgc aggacaacgt cgagcctttc tcctttgccg aggtggagga
     2341 catcgtctcc accgagctgg gcgtgcggct ctccaaggcc ttctccgact tcgactcggt
     2401 tcccctggcg gcggcctcgc tcggccaggt ccatcgcgcc gccctgcgcg acggccgcct
     2461 ggtggcggtc aaggtgcagc ggccgggtat ccgccagacg atcctggagg atctcgaggc
     2521 attcgacgag atcgccggct tcatcgaccg gcacaccgag atgggccgga agttcgcctt
     2581 caaggatatg ctggaggagt tccgcaagac gttactgcgc gagctcgatt accgtcagga
     2641 ggcgctgaac ctgtcgactc tgcggaagaa cctccgcgag tacccgcgcc tggtgatccc
     2701 gcagccgatc gaggactaca gcaccgcccg cgtgctgacc atggagtacg tgcgggggac
     2761 caagatcacc gatctctccc ccctggcgcg catcgacctg aacggccagg tcctggccga
     2821 ggatctctgc cgggcctacc tggaccagat cctggtggac ggcttcttcc acgccgatcc
     2881 ccaccctggg aacctgctgg tcaccgacga cggccggctg gcgctgctcg acctgggcat
     2941 ggtggcgcgc atcgatccgc agatccagga gcggctgctc aagctgctgc tggcggtcat
     3001 cgacggccgc ggccacgagg cggcccagat cgccatccag atcggcaccc cgctcgaagg
     3061 gcacgatgaa aaccgcttcc ggcgcgaggt ggccgacctg gtggggagct accagagtgt
     3121 gccgcggcgc gaggtccagg tggggaaggt gatcctgagc ctggcgcggc tgtcggcgga
     3181 cagcggcgtg cggccggcgc cggagctgac catgctcggc cgtgccctcc tgtgcctcga
     3241 cgagagcgcc cgcgccctcg accccgactt cgatcccaac gccacgatgc ggcgttacac
     3301 cgacgccgtc ctctcccggc acatgttcaa gcggctgtcg ccggagagct tcgtgtcgtc
     3361 gacgctggag atgtacgagt tcgtccagca gttcccgtcg cggctcaatt ccgtgctgga
     3421 gacgctggcg ggcaaccggc tggagctcaa ggtcaacgcc ttcgacgaga cccgcctgat
     3481 gacgaacctg cagaagatcg ccaaccgcat cgccatgggg ctggtgctgg cggcgctcat
     3541 cgtcggcgcc gcgctgatga tgcaggtgcg gaccaagttc acgctgttcg gctatccggg
     3601 catcgccatg ctgctcttcc tgatggccgc cgcctgcggt ttcgtcctgg tcctgagcat
     3661 cctgttcagc gacgactggg gcatctcgcg caaacgccag cactgagccg ctacatcccc
     3721 tgggtgcccc cctccttcag cacgaagtcg gggttgccgt agaggatgta gttggcccag
     3781 tccttggtcc cctgcgcctt gcggatggcc tcgcggccga cctggagcgc ttggccgagc
     3841 gggcggccac gtaggatctg ctcgtagaaa gccagggaga agatctcggc ttcgcggtcc
     3901 ccgaccggcc agtaggtgcc gatgaagttg gcgattccac cgcgcaggaa cgcttcggcg
     3961 aagcttaccg cctctgtgat ccgaactctc aaatccctgg ggcggagccc cctggcgcgc
     4021 gggggtgccg tgttgcgcga tccgcggcgc acgcgggcgg attcacaggc attgaagaag
     4081 accagcgtcg gcaggttccc cacaccgacg aggtcggcgc cggtcagacg ggcctcgttg
     4141 tggcatagca gaccgctgtg ctccgggctg gcgggatcaa actcggcgtg gccggcatag
     4201 tggatcacgt cgtactttcc tgagctgaag gccttgagca gcgccggccg tgtcgcctcg
     4261 ctccctcgga taatatccat ctggagcccc ttgaggcggc cgaggagcct ctgcagcgcg
     4321 gcaccctcgg cctccgctcc ctccaggtcc tccgtcgggt tgagtaccag gaggatgttc
     4381 agaatcccgt cgtggatcct ctgctcgagc cacttggcaa cggacaggtt gtcggcggca
     4441 tagcggcggc tcatcccttc ctcggcggcg gggaaccaga tgtccggtcc cccggggaaa
     4501 gtgatctgcc ccggcgagct ctggcggtcc gcgtgcaggg cgaggatctc ccagggcatc
     4561 cgggagagga aggcgtcgtg gaccaccacc aggtgccggt cccggtgacg caggaggacc
     4621 gttctcacgt cttcggcgag tatcaggtcg gcgagctccg tcccgggctc ggtcaggtcc
     4681 atcttcattt ccttggcctt gagccgttcg acgcaggcct ggatctccac ctccgacacc
     4741 ggccgggtgc cgctcacgac catcgcctgg ctgccggcgg tgagcagggc cgagcggacc
     4801 tcgaagccgg ggccgccgtc cctggggtcc ctttcccctt cccggcgaac gatcaggtag
     4861 accgggtcct tgcgttccgg gcccgacctg gacacgggag gtagaggggg agacggcagc
     4921 tccacctcgt ccatggagat ctggacgccc tggcagagcc cggaggtcga caggcggagg
     4981 aactcctggc gggcggcggc gtagcgctcc cggtctttct cgcagatcac cacacgatgg
     5041 aagaagtggt tccggtcggc gtcgaggaga ccgcggagga agccggtcag gaggctgtgg
     5101 agcaccgagg aggcacgctc ccccgagccg acgccaaaga gcacggtgga gaactcctcg
     5161 acccgggtgt tgatgaaggt gcggatgacg ttctccgccg ccgtctgcag cacctcctcg
     5221 ttgaaacggt cgaacgagcc gagaccgacg aaggcgatgt ggtccgcggc cagggggtgc
     5281 ttgccggtcg gcatcaagaa gatctcgccg acttcgccgg agaacatccg gcgccggaac
     5341 agctcggtga tcgtaccgcc cagccgctcg tccagggcgc gcgccggacc gctgggcgcc
     5401 acgtcgcgga agatgccgag ggcgatggcc cgcgtgtccg cctcggtgat gctgcccaag
     5461 gcgaggcgca ggtcgaggcg gtgctgccgg gtctggttga tgacgatgcg gttgccgggg
     5521 aactggctgg gctcgagtgg caggccgtca gccgcctgcg acggcgtctg ggccaggccg
     5581 ctctcgcgcg gctggggcgc caccacctcg tcgaggaggt ggcgcagctc ccgctggctc
     5641 agggcgccat cgcgctggga acggtacggg ttcttccgca agtcctcttc gagacagagc
     5701 tgcgggggct cgctctgcac cacgatgggg ggggagcccg gcagggcgtt ggtggtgccg
     5761 aggcccagga tctccgcgat ggcgtgagcg acccggggat tcgtgggcag agtgccgtga
     5821 gaatcctcga cataccaggt cctggcgccg ggcagcaggg cgagatcgac cggcacgaca
     5881 ccgtccccgt cgggcgagaa ctcgtaggag aagccgccgt ccggccggcg gacgagaccg
     5941 gtgaccgtct tctggttcac cccggcgatg aggaagaaac gctcgtcggc cggggcgagc
     6001 gccttttgga cctctcgcac tcgttccagg agctcctgcc gaggccggag ctcgtcttct
     6061 ggcggccaca tggggagctt gtagaggttc acctcgccga aacggtcggg tgatggcagc
     6121 aattgggtga gcccggggaa ggt
//
LOCUS       DASPJI010000829         2398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_454364, whole genome shotgun sequence.
ACCESSION   DASPJI010000829 DASPJI010000000
VERSION     DASPJI010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454364"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="VEL74_23375"
     CDS             <1..1519
                     /locus_tag="VEL74_23375"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYG65543.1"
                     /translation="GAWSVPRLNGEIYTQKPPLLFWSINAAALLTGELDETAARIPLA
                     IAAIGSLLLVYRIGERLFSRRAAWITVAAYGTCLRVLWQGRFGQIDMLLAVFVTLGVW
                     FWVRGYTERKPHLYYLFFLSAGLATLAKGPVGLLPPLLSILAFLALTGQGGEIRRLRL
                     GTGLLVWAAVVLAWLLPAAQSGGREYLEQIVYKQNVTRYANPWHHHRPFYYYLTVVPA
                     EYFPWSFLLPTALVLGWRRIVGGRTGLRQMRSPVLPFDARHGFLFALCWMVITILFFS
                     LSSGKRSVYVLTMYPAMALMIGAVLDRLALDWPRDRRWLTVPFAALTLLAILIVLGLP
                     IAGRGRPEAAPMGGDRLVWMVTAAFLSLLLRAAGAWWQSRSGRAGRAAAALAAGMGTL
                     MLVTALLLLPRFDAVKSARDLARVLRREMGPGEPYGIYPRLDSTFLFYSRRFAVDLNS
                     EAELQEFVRRPGRIWLLIRRDELPKLQQPLPPLVEVARDADVREGYVLLTHPAAH"
     gene            1593..2132
                     /locus_tag="VEL74_23380"
     CDS             1593..2132
                     /locus_tag="VEL74_23380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65544.1"
                     /translation="MTTNRSLSLVLLILPLLIVAALRLSAQPPAAPPVDLLPQAHTVV
                     DLRHVGTAMWAWLADQRSQNPRKVATESQGKAAPATAPISEVPVISHQELTKILVPRY
                     IASVPEKDGWGHRYEYRMNVANPQAEHPIALRSPGQDGRFSTDTYEIGGYFHDEVAQD
                     LAWMDGYFVRWPEPKKPAK"
     gene            complement(2186..>2398)
                     /locus_tag="VEL74_23385"
     CDS             complement(2186..>2398)
                     /locus_tag="VEL74_23385"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HYG65545.1"
                     /translation="QTDAGMTGPYESIIGMRIDKVLKRFILQTPSSFEVAKRDVRLAG
                     AVIDVDEETGKARSIERLLVPATSPA"
BASE COUNT          309 a          941 c          737 g          411 t
ORIGIN      
        1 cggcgcctgg tcggtgccgc gcctcaacgg cgagatctat acccagaagc cgcccctcct
       61 cttctggtcc atcaacgccg ccgccctgct caccggagag ctggacgaga ccgcggcccg
      121 catccctctg gccatcgccg ccatcggctc cctgctgctg gtctaccgga tcggcgagcg
      181 cctgttctcg cgccgcgccg cctggatcac ggtcgccgcc tatggcacct gcctgcgggt
      241 cctctggcag ggccgcttcg ggcagatcga catgctgctc gccgtcttcg tcaccctggg
      301 cgtctggttc tgggtccgcg gctacaccga gaggaagcct cacctctact atctcttctt
      361 cctctccgcc ggcctggcca ccctggccaa ggggccggtg gggctcctgc cccccctgct
      421 ctcgatcctc gccttcctgg ccctgaccgg acaagggggc gagatccggc gcctgcgcct
      481 cggcaccggg ctcctcgtct gggcggccgt ggtcctcgcc tggctgctcc cggccgccca
      541 gtcgggaggc cgggagtacc tggagcagat cgtctacaag cagaacgtca cccgctacgc
      601 caacccctgg caccaccacc ggcctttcta ctactacctg acggtggtcc cggccgagta
      661 tttcccctgg tccttcctcc tgcccacggc gttggtcctc ggctggcgcc gcatcgtggg
      721 aggccgcacc ggcctgcgcc agatgcgctc gcccgttctc cccttcgacg cgcgccacgg
      781 cttcctcttc gccctctgct ggatggtgat aaccatcctc ttcttcagcc tctcctcggg
      841 caagcgctcc gtctacgtcc tcaccatgta cccggcgatg gcgttgatga tcggggccgt
      901 cctcgaccgt ctcgccctgg actggccgcg ggaccgccgc tggctgaccg tccccttcgc
      961 cgccctgacc ctcctcgcta tcctcatcgt cctcggcctg cccatcgccg gacggggccg
     1021 gcccgaggcc gcgcccatgg gcggcgaccg cctcgtctgg atggtcaccg cggccttcct
     1081 ctcactcctc ctgcgggccg ccggggcctg gtggcagtcc cgctccggcc gcgccggccg
     1141 cgccgccgcc gccctggccg ccggcatggg cactctgatg ctcgtcaccg ccctcctcct
     1201 cctcccccgc ttcgacgcag tcaagtccgc gcgtgatctc gcccgcgtgc tccgccggga
     1261 gatgggtccg ggcgagccct acggcatcta cccccggctg gactccacgt tcctcttcta
     1321 cagccggcgc ttcgccgtgg atctgaacag cgaggccgag ctccaggagt ttgtccgccg
     1381 ccccggccgg atctggctcc tcatccggcg cgacgagctt cccaagctcc aacagcccct
     1441 gccgccgctg gtggaggtcg cccgcgacgc cgacgtccgg gagggctacg tgctcctcac
     1501 ccacccagcc gcacactgac gtaccggtgt accactcctg tggtacactt ctctccgcaa
     1561 gggccctgca ctcaacccaa ggagagattc caatgaccac caaccgttcc ttgtcgctcg
     1621 tcctccttat cctgccgctt ctgatcgtcg cggcgctccg cctgtccgcc cagccgccgg
     1681 cggcaccacc cgtagacctc ctgccgcagg cgcacaccgt ggtcgacctc cgtcacgtgg
     1741 gtacggccat gtgggcatgg ctggcggatc agcgcagcca gaatccccgc aaggtggcga
     1801 ccgagtccca ggggaaggcc gcaccggcga ccgcccccat cagtgaggtg ccggtgatct
     1861 cccaccagga gctcaccaag atcctggtgc cccgctacat cgcctccgtc ccggagaagg
     1921 acggctgggg ccaccggtac gagtaccgga tgaacgtcgc caatccccag gcggagcacc
     1981 cgatcgctct ccgcagcccg ggccaggatg gacggttctc gaccgacacc tatgagatcg
     2041 gagggtactt ccacgacgag gtcgcccagg acctcgcctg gatggacggc tacttcgtcc
     2101 gctggccgga gccgaagaag ccggcgaagt aggtaggagg gtgagacggc gccggcccgc
     2161 gggcgggtcc ggcgccgctc gggtcctagg ccggcgaggt ggccggcacc agcaggcgct
     2221 cgatggagcg cgccttgccg gtctcctcgt ccacgtcgat gacggcgccg gcgaggcgga
     2281 cgtcccgctt ggccacctcg aaggaggagg gggtctgcag gatgaaccgc ttgaggacct
     2341 tgtcgatccg catgccgatg atcgactcgt aggggccggt catgcccgcg tcggtctg
//
LOCUS       DASPJI010000830         4185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_682835, whole genome shotgun sequence.
ACCESSION   DASPJI010000830 DASPJI010000000
VERSION     DASPJI010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682835"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="VEL74_23390"
     CDS             <1..1497
                     /locus_tag="VEL74_23390"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HYG65546.1"
                     /translation="GSLAYMALLCVLLWSVGESLVRSRRIDFSTSLDALRSARLGPRG
                     GRALLHGLGYGAGLAGLWLVLFAAAAALPGISPLGARFPLPLFSPAETLFPVQYLLDA
                     AGTLLAIGLASYLPARWAPFAAVLLAPLFSPVSPLLYFDPRPLGLILGFAPSAVLVYA
                     FHRSGLTAMLTAAIVSALFPAALLSGLHVAWAPATLAASMGLLAVVGLLGVWGLSRPA
                     EVDAEGGRPPAFVRRLRAKRDRRYEMDLLAQMQRGLLPCQMLVADGYEIATHSAAHSG
                     SEAAAEGNLYDLLQDDAGRLWVVTADVAGQGYSRAVAQAMIKAVLVSSMDGARERSDR
                     LPSEILAETRRVLYREGPACESAALTLGRIDPRTGRAVVSCAGAPPPLLLAGGEVSPL
                     STPGPPLTPGSTAGYQDAELLLPPGGTLLFCSAGLFGAVDWSGIPFGQDHVRNELRSV
                     GSEPAAAILHALLDGWRRHTNLEEPPAGNTILALRRRTREELTGDAAA"
     gene            1481..3796
                     /locus_tag="VEL74_23395"
     CDS             1481..3796
                     /locus_tag="VEL74_23395"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HYG65547.1"
                     /translation="MTRPPEQDDRRDLYRGAAWGLLALLAGLLLAWWLLPEWRTDFTL
                     TDGAARQRYRDLAVRAGLRPVPGEERVTALGGYYFQAYRALGEMGDEWLAATHTPVLL
                     EVSHEVRQPESENARRFEVRFSPLGVPHGLCWGRWDNDRDESARFEAMARLLLEPGEL
                     LGPPHRKPGEDTVRFAVTGSRAPTYLVASSGPCLVEVVRRVDAPGLVQGHESALGSAL
                     LFLGVVVLFLVFALRARLSVVNGLLLALAAVLSVDFTHLSKLPSSLPVELPPQVTWIF
                     YLGVLMGAGPTLPAILLAWPVGESLLRLVRPETVTDMDELRQGRLRRRTGRALLSGFG
                     WGLALAGFRLGLLALAIPIPGVSPDSLSVILPPSSLTSGALGESLVLAAWMSLVVALA
                     VRLLPARWAVWAAAVAGGGLLELLRFTHLSYYSFIPVTPWPVSLCVNVVLAGVLSYVT
                     LRFGLAGLLASASSYLLVPTALLASRAISWWPVSFTLTAAVPLAILVLGWRGATRAGE
                     PGPRRVWQPAFLARLEEERHMREMDLLARIQAGLLPREMPRVEGWDLSARSVLAQEAG
                     GDLYDFLPDGEGRLWIAAGDVAGHGPSCAIALAMTKAALATLVTASRSPAEVLRRVDQ
                     VLRSGSANRRFTTLALLRLDPRTGEAVLANAAHPYPLLAADGGVSELELPGLPLGQGP
                     RREYREISFQVPSGGVLAFVSDGLLEAMDASGTPYGFDAAKDVLQSSAGRSAGEIVET
                     LMAAWRHHLGSRPTDDDTTVVIVKRLDAERG"
     gene            3825..>4185
                     /locus_tag="VEL74_23400"
     CDS             3825..>4185
                     /locus_tag="VEL74_23400"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYG65548.1"
                     /translation="MTLYDTLIVGGGPAGLTAALHLAWHDRKVLVIDRVSGPLFYTLE
                     LLHNVPGMPAVSGAEIQKRLRKQAQEMGAEVVRGNVVHAEGSEGDFRLSGEKGEEWRG
                     KTVLLATGVARHHPLVDG"
BASE COUNT          464 a         1566 c         1436 g          719 t
ORIGIN      
        1 ggatcgctgg cctacatggc gctgctctgc gtgctcctct ggtcggtcgg agagtcgctg
       61 gtgcgctccc gccggatcga cttctccacc agcctggacg ccctgcggtc ggcgcgcctg
      121 ggcccgcgtg gcggcagggc gctgctccac ggcctcggct acggcgccgg gctcgcgggt
      181 ctgtggctcg tcctgttcgc cgccgccgcc gccctgccgg ggatctcacc gctcggagcg
      241 aggtttcccc tgcctctctt ctcgccggcg gagaccctgt tcccggtgca gtacctgctg
      301 gacgcggccg gtaccctgct cgccatcggc ctcgcctcct atctgccggc ccgctgggcc
      361 cctttcgcgg cggtcctctt ggcgcccctg ttctctcctg tctcccccct gctctacttc
      421 gatccccggc cgctggggct gatcctcggc ttcgctccga gcgccgtgct ggtctacgcc
      481 ttccaccgct cgggcctgac cgccatgctc accgccgcga tcgtctcggc cctgttcccg
      541 gcggccctcc tgtcgggtct ccacgtggcc tgggcaccgg cgacgctcgc cgccagcatg
      601 ggcctgctgg cggtggtcgg gctgctgggc gtctgggggc tctcgcgtcc tgccgaggtg
      661 gatgcagagg ggggccggcc gccggcgttc gtccgccgtc tgcgggcgaa gcgggaccgc
      721 cggtacgaga tggacctcct ggcccagatg caacgcggcc tgttgccgtg ccagatgctg
      781 gtggccgacg gttacgagat cgccacgcat tcggccgccc actccggcag cgaggccgcg
      841 gcggagggca acctctacga cctcctccag gacgatgctg gccgcctgtg ggtcgtcacc
      901 gccgacgtcg ccggccaggg ctactcgcgc gccgtggccc aggccatgat caaggcggtg
      961 ctggtcagct ccatggacgg cgcccgggag cggagcgacc gcctgccctc cgaaatcctc
     1021 gccgagaccc gccgcgtgct ctaccgggag ggacccgcct gcgagtccgc cgccctgacg
     1081 ctcggtcgga tcgacccgcg gaccgggagg gccgtagtga gctgcgccgg cgcgccgcca
     1141 cccctgctcc tggcaggcgg cgaggtctct cccctctcca cccccggccc gccgctcacg
     1201 ccgggatcca ccgccggata ccaggatgcc gagctcctcc tgccgcccgg cggcaccctc
     1261 ctgttctgct ccgcgggcct cttcggagcc gtggactgga gcggcatccc ctttggccag
     1321 gaccatgtcc ggaacgagct tcgctccgta ggctccgagc ccgcggccgc catcctccac
     1381 gccctcctcg acggctggcg gcggcacacc aatctggagg agccgcccgc cgggaacacc
     1441 atcttggcgc tccggcggcg gacgcgagag gaactgaccg gtgacgcggc cgcctgagca
     1501 ggacgaccgg agggacctct accgcggggc cgcctgggga ctgctggcgc tcctcgccgg
     1561 cctgctcctg gcctggtggc tcctgcccga gtggcggacc gacttcaccc tgacggacgg
     1621 cgcggcgcgc cagcgttacc gcgacctggc ggtccgtgcc ggcctgcgcc cggttcccgg
     1681 ggaggagcgg gtgaccgcgc ttggggggta ctacttccag gcgtaccgag ccctgggtga
     1741 gatgggtgac gagtggctcg cggcgacgca cacccccgtc ctgctcgagg tgtcgcacga
     1801 ggtccggcag ccggagagtg aaaacgcccg gcggttcgag gtccgcttct cgccgcttgg
     1861 tgtgccgcac ggcctctgct ggggacgctg ggacaacgac cgcgacgaga gcgcccgttt
     1921 cgaggccatg gcccgcctgc tcctggaacc gggcgagttg ctcggccctc cgcaccggaa
     1981 gcctggagag gatacggtcc gcttcgccgt gaccggaagc cgtgccccta cctaccttgt
     2041 cgcctcgtcc gggccctgcc tggtcgaggt cgtgcgccgg gtggatgcgc ccggcctcgt
     2101 gcaggggcat gagagcgcgc tcggctccgc gctgctgttc ctgggagtcg tggtcctctt
     2161 cctggtcttt gccctgcgag cccggctcag cgtcgtcaac ggcctgctgc tggcgctcgc
     2221 cgcggtgctg tcggtggact tcacccacct ctctaaactc ccctcctcac tccccgtcga
     2281 gttgccgccg caggtcactt ggatcttcta cctgggagtc ctcatgggag cggggccgac
     2341 gctgccagcg atccttctgg cctggcccgt tggtgagtcg ctgctccgcc tggttcgtcc
     2401 cgagaccgta acggacatgg acgagctccg ccagggtcgt ctccggcgaa gaaccgggcg
     2461 ggcgctcctc tccggcttcg gctggggcct cgccctggca ggcttccgcc tcggcctgct
     2521 cgctctcgcc atcccgatcc ccggagtatc gccggatagc ttgagcgtca tcctgccgcc
     2581 gtcctccctc acctcaggcg ctctgggcga gagcctggtg ctcgcggctt ggatgtcgct
     2641 cgtcgtcgct ctcgccgtcc ggctgctgcc ggcccgttgg gcggtctggg cggcggccgt
     2701 ggccggcggc gggctgttgg agctcctccg tttcacccat ttgtcctact actccttcat
     2761 tcctgtgact ccatggccgg tctccctgtg tgtcaatgtc gtcctggccg gtgtcctctc
     2821 ctacgtcact ctgcgcttcg gcctcgccgg tctgctggcg tctgcgtcgt cctacctgct
     2881 ggtccccacc gctctcctgg ccagcagggc gatctcctgg tggccggtca gctttacatt
     2941 gacggccgcc gtgccgctgg cgatcctggt gctgggatgg cggggagcca cgcgggccgg
     3001 cgagccgggt ccccgccggg tgtggcagcc ggcgttcctc gcgcggctcg aagaagagcg
     3061 ccacatgcgc gagatggacc tgctggcgcg catccaggcc gggttgctgc cacgggagat
     3121 gccccgcgtc gaggggtggg acctctccgc ccgctcggtc ctggcccagg aggcgggtgg
     3181 agacctctat gacttcctcc cggatggcga ggggcggctc tggatcgccg cgggcgacgt
     3241 ggcgggccac ggtccgagct gtgccatcgc cctggccatg accaaggcgg ctctcgccac
     3301 cctggtgacg gcctcccgct ccccggccga agtcttacgg cgcgttgacc aagtcttgcg
     3361 gtcggggagc gcgaaccgcc gcttcactac cttggcgctg ctccgcctcg atccacggac
     3421 cggcgaagcc gtgctggcca acgccgccca tccctacccc ctgctggcgg cggacggcgg
     3481 agtgtccgag ctggagctgc ccgggctgcc gctgggccag gggccccgcc gcgagtaccg
     3541 ggagatctcc ttccaggtgc cgtccggcgg cgtgctcgcc ttcgtctccg acggtctgct
     3601 cgaagccatg gatgcctctg gcacgcccta cgggttcgac gccgccaagg acgtcctcca
     3661 gtcgtcggcc ggccggtccg ccggcgagat cgtggaaacg ctgatggctg cctggcgcca
     3721 ccatctgggc tcccggccga ccgacgacga cacgaccgtg gtgatcgtga aacgcctgga
     3781 cgctgagcga ggctagaatg cggcctgagc ttcccgaggc acacatgacc ctctacgaca
     3841 cgctgatcgt cggtggcggc ccggcagggc tgacggccgc tctccatctc gcctggcacg
     3901 accgcaaggt gctggtgatc gaccgcgtct ccgggccgct cttctacacc ctggagctgc
     3961 tccacaacgt gccgggcatg cccgccgtct ccggcgccga gatccagaag cggctcagga
     4021 agcaggcgca ggagatgggg gccgaggtcg tacgcggcaa cgtcgtccac gccgagggct
     4081 cggaggggga cttccggctg tccggtgaga aaggcgagga gtggcgcggc aagaccgtgc
     4141 tcctcgccac cggcgtcgcc cgccaccacc cgctcgtgga cggcg
//
LOCUS       DASPJI010000831         4449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_682872, whole genome shotgun sequence.
ACCESSION   DASPJI010000831 DASPJI010000000
VERSION     DASPJI010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682872"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2184
                     /locus_tag="VEL74_23405"
     CDS             <1..2184
                     /locus_tag="VEL74_23405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HYG65549.1"
                     /translation="LAALAFLGLAWGRRAGLLLACCALGLLVAVVRGPFRESGPDLDR
                     PLDAVVRVAGHWTADDEGWSAPARLLRCRQGLEVSAPPLEVLVHIPGPDEEPPAFGTV
                     LRLRGYLARSVGFANRVPVQPGPWRLRVKSRSLMEVEEPPGRIAQLSSTLRRRVETAY
                     RVAAGEETGPGIALVRALVLGDASALPLEWKRGLRVTGVYHLMSVSGVHVALVAGAVW
                     LLGFWLPRSIRLLLMLAAIGGYLLLVGPLPALVRAALMGLLAVSALLAERPPAAANAL
                     GWAVIVLVLDRPDLVNAPAFQLTVSATAGLLLATPALARRWHRLPRWLAGSLAASVAA
                     QLATLPWSLPRFHLLSPLAPLLNLPAVPWTGLALGVALVWTPLALLAPSLAGALVPVL
                     DRLAAPFSWPAETGPAVWLPVPLLVSPPAAWLAAGLLGLALLWRPRHRLLRWTALPVA
                     ALAGTAILAWPWLPGGRAPARGVEISLLDVGQGDAILLRDGDRAVLVDGGGWRKGDLG
                     GRVLLPALLGEEIRSLDALVMTHPDRDHCGGLLDIAAYIPVREVWMSPGWEGEECADR
                     LRALPGARTRFLWAGERGAVGRWRLRILHPERDEERGGNERSLVILAEALERRALLTG
                     DIESWAEMRLLACCERDLRVDFLKVAHHGSKTSTTTSFLEAARPRLALVSAGPGNIYY
                     HPSPVVVERLEEHGAGVLRTDRDGLIRIALLEDGGTRIELPGAPR"
     gene            complement(2195..>4449)
                     /locus_tag="VEL74_23410"
     CDS             complement(2195..>4449)
                     /locus_tag="VEL74_23410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG65550.1"
                     /translation="GVKEETQRWPTSYPDFVDLRQRARSLEGLAVRTENRHFKLREPE
                     GAALIGGEMVSANYFEVLGVRPAHGRGFLPDEDRTPGGNPVAILSDGLWHRAFGADPG
                     MIGSTVELNELLYTVVGVMPAGFRSSTDGVDVWLPISMATTISPTYLEDRAYRSLTMI
                     GRLRQGITVEDAQKELSGVAEQLAREYPDSNEDIGVEVLPLRDSILGNVRPLLWTLLG
                     GSAFVLLISCTNIANLLLAKAVSRQREISLRVVLGAGRRRLIRQLLTESAFLGILGCI
                     LGLLVASLSVGLIVRMSGSGMSGSELRGFVSNTLDPLAVVVILVVSIFSGLLFGLAPA
                     LFASRTDSKGLMSEGARNATAGTGSRRLQGLLIVGEVALAVALLVGVGLMTKSFWQQS
                     RIDLGFKADHLLTLRMYTQSQRVATPESLRQVISDVLARVEAVPGVSSVAVSGPGMPT
                     DEFYGLYFELEGRRQDDQLLALRHHVSPGYFSTLAVPLLRGRVFTPQDTAETTPVVVV
                     SNSLAKQFWPGQDPIGKRLKAAPTPWLTVVGVVADVRQNGLSEGEWPAPDLYLPALQL
                     APRTPPVLNLLVRTSVPPDTLGVTIQRELKRFIPELPVYEIMTMEARLSDQMANRRLL
                     VLMMGLFSLAALLLAAVGIYGVTSYAVSQRTREFGIRLALGAQPREVIWAALARGFGL
                     ALTGLSLGLIAGLALTRLIKSQLLGTIALDPILLASTVLVLLVVTLVANYIPARRAVR
                     ADPVIALREQ"
BASE COUNT          618 a         1540 c         1594 g          697 t
ORIGIN      
        1 ctggccgccc tcgccttcct gggtcttgcc tggggccgcc gcgccggtct gctcctcgcc
       61 tgctgtgccc tcggcctcct ggtcgcggtg gtccgcggac ctttccggga gagcgggccc
      121 gacctcgacc ggccgcttga cgcggtggtg cgggtggccg gccactggac ggccgacgac
      181 gaggggtggt ccgctccggc ccgcctcctc cgctgccgcc aggggctgga ggtgtcggcg
      241 cctcccctgg aggtgctcgt gcacatcccc ggcccggacg aggagccgcc cgcgttcggg
      301 acggttctcc ggctgagagg atatctcgcc cgctcggtgg gtttcgccaa ccgcgtccct
      361 gtgcagcccg gcccctggcg gctgcgggtc aagagccgca gcctgatgga ggtggaggag
      421 ccgccaggcc ggatcgccca gctctccagc acgctccggc gccgcgtcga gacggcgtac
      481 cgcgttgctg caggcgagga gaccgggccg gggatcgcgc tggtgcgggc cctggtgctg
      541 ggggacgcct cggccctgcc tctggagtgg aagcgggggc tgcgcgtcac cggcgtctac
      601 cacctgatgt cggtctcggg tgtccacgtg gcgctggtcg ccggcgcggt ctggctcctc
      661 ggcttctggc tgccgcggag catccgcctg ctcctgatgc tggcggcgat cggaggctac
      721 ctgctgctgg tcggtccgct cccggccctg gtacgggccg cgctcatggg gctcctggcg
      781 gtctcggcgc tcctcgccga gcgcccgccg gcggccgcca atgctctggg ctgggcggtg
      841 atcgtcctgg tcctggaccg tccggacctg gtgaacgccc cggccttcca gctcacagta
      901 tcggcgacgg cggggctgct gctcgccacc ccggccctgg cccggaggtg gcaccgcctg
      961 ccacgctggc tggcgggctc gctcgcggcg agcgtcgccg cgcagctcgc aaccttgccg
     1021 tggtccttgc cacgcttcca tctcctgtcg ccgctcgcgc cgctgctcaa tctaccggcc
     1081 gtgccatgga ccggcctcgc tctcggggtg gccctggtat ggacacctct ggccctgctg
     1141 gctccgtcgc tggccggtgc cctggtaccg gtgctcgatc ggctggccgc accgttctcc
     1201 tggccggcgg agaccgggcc agccgtctgg ctgccggtgc cgctcctggt ctcgccgcca
     1261 gcggcctggc tcgccgcggg cctcctcggg ctcgccctgc tctggcgtcc gcgccaccgg
     1321 ctgttgcggt ggacggccct gcccgtggcc gccctcgccg ggaccgcgat cctggcctgg
     1381 ccttggctgc ccggcgggcg tgcgcccgcc cgcggcgtcg agatctccct tctggacgta
     1441 ggccaggggg acgccatcct gctgcgcgac ggcgaccggg cggtcctggt cgatggcggt
     1501 ggctggcgaa agggggacct gggcgggcgg gtactccttc ccgccctcct gggggaggag
     1561 atccgaagtc tggatgctct cgtgatgacc caccctgacc gcgaccattg cggcggcctg
     1621 ctggacatcg ccgcctacat tccggtgcgc gaggtctgga tgtcgcctgg atgggagggc
     1681 gaggagtgcg ccgatcggct gcgagccctg ccgggagccc ggacgcgctt cctctgggcg
     1741 ggggagaggg gggcggtcgg gcgctggcgg ctgcggatcc tgcatcccga gcgcgatgag
     1801 gagcgcggag ggaacgagcg gtcgctggtg atcctcgccg aggcgctgga gcgccgggcg
     1861 ctcctcaccg gcgacatcga gagctgggcc gagatgcgcc tcctcgcctg ctgcgagcgc
     1921 gacctgcggg tggacttcct caaggtagcc caccatggga gcaagacctc caccaccacc
     1981 tcgttcctgg aagcggcgag accccgcctc gccctggtct cggccggacc ggggaacatc
     2041 tattaccacc cctccccggt ggtcgtggag cgcctcgaag agcatggcgc cggtgtgctg
     2101 cgcaccgacc gggacgggct gatccggatc gcgctgctgg aggacggcgg gacccggatc
     2161 gagttgccgg gggcaccgcg gtgacggaga gcccctattg ctcgcggagc gcgataaccg
     2221 ggtcggcgcg caccgcccgg cgcgccggga tgtagttggc gaccagagta accacgagca
     2281 gcaccaggac ggtgctcgcc aggaggatcg gatccagagc gatcgtgccg aggagctgac
     2341 tcttgatcag acgagtcagg gccaggccgg cgatgagccc gagggagagg ccggtcagcg
     2401 ccagaccgaa ccccctggcc agcgccgccc agattacctc ccttggctgg gcccccaggg
     2461 ccagccggat accgaactcg cgggtgcgtt gcgacaccgc gtaggacgtg acgccataga
     2521 tccccaccgc cgccaggagc agcgcggcca aggaaaagag gcccatcatc aagacgagca
     2581 gacgccggtt cgccatctga tccgacagtc tcgcctccat agtcatgatc tcgtagaccg
     2641 ggagctcggg gatgaaccgt ttgagctctc tctggatggt tactccgagg gtatctggcg
     2701 gcaccgacgt gcggacgagc aggttgagca ccggtggtgt ccggggcgcc agttgcagcg
     2761 cggggagata caggtcggga gcaggccact ccccttcgct gagtccgttc tggcgcacgt
     2821 cggcgaccac gcccaccacc gtgagccatg gggtcggcgc ggccttgagg cgcttgccga
     2881 tggggtcctg gcctggccag aactgcttgg ccagcgaatt gctgaccacg acgacgggcg
     2941 tggtctctgc cgtgtcctgg ggcgtgaaga cgcgcccgcg caggagaggc accgcgagag
     3001 tggagaagta gccgggactg acgtggtggc ggagggcgag gagctggtcg tcctgccggc
     3061 ggccctcgag ctcgaagtag aggccataga actcgtccgt cggcataccg ggaccggata
     3121 ccgccacgga cgagactccg gggaccgctt cgacacgcgc gagcacgtcg ctgatcactt
     3181 ggcgcaggct ttccggggtg gccactctct ggctttgggt atacatccgc agagtcagca
     3241 ggtggtccgc cttgaagccg agatcgatcc ggctctgctg ccagaacgac ttggtcatca
     3301 ggccgactcc cacgaggagg gcgactgcca gggcgacctc accgacgatc agcaggccct
     3361 gcaaccggcg gcttcccgtt ccggcggtgg cattgcgtgc cccctccgac atcagccctt
     3421 tgctatcggt gcgcgaggcg aagagcgccg gcgccaatcc gaagagaagg cctgagaaaa
     3481 tcgacacgac gaggatgacg accaccgcaa gcgggtcgag ggtgttgctg acgaaccccc
     3541 gcaactcgga gccgctcatc cccgagccgc tcatccggac gatgagcccg acgctcagtg
     3601 aggcgacgag gaggcccagg atgcagccca ggattcccag gaacgcgctc tcggtcagga
     3661 gctggcggat caacctccgg cggcccgccc ccagcactac gcgcagactg atctcgcgct
     3721 ggcggctcac cgccttggcc agcaggaggt tcgcgatgtt ggtgcaactg atgagcagga
     3781 cgaacgccga gccgccgagt agcgtccaga gcagcggccg tacattgccc agaatagagt
     3841 cccgcaacgg caggacctcc accccgatgt cctcgttgct gtcggggtac tcgcgggcga
     3901 gctgctcggc aacgccggag agctccttct gggcatcttc gaccgtgatg ccctgcctga
     3961 gacggccgat cattgtgagg gaccggtacg cccggtcctc gaggtaggtc gggctgatgg
     4021 tggtcgccat ggaaatgggc agccagacgt cgacaccatc tgtcgagctg cggaagcctg
     4081 ccggcatgac cccgacgacc gtatagagga gctcattgag ctcgaccgtg ctaccgatca
     4141 tccccgggtc cgcgccgaag gcgcggtgcc agagcccatc actcaggatc gccaccggat
     4201 tgcctcccgg ggtcctgtcc tcgtccggca ggaagccgcg cccgtgggcc gggcggactc
     4261 ccagcacttc gaagtagttg gcggacacca tctcgccgcc gatgagcgcg gcgccctcgg
     4321 gctcgcggag cttgaagtgc cggttctccg tccgcacggc aagcccttcg agagagcggg
     4381 cgcgctggcg caggtcgacg aagtcgggat aggaggtcgg ccacctctgg gtctcctcct
     4441 tgacgccgc
//
LOCUS       DASPJI010000832         7780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_797425, whole genome shotgun sequence.
ACCESSION   DASPJI010000832 DASPJI010000000
VERSION     DASPJI010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7780)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797425"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VEL74_23415"
     CDS             complement(<1..887)
                     /locus_tag="VEL74_23415"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HYG65551.1"
                     /translation="MPPHRESREPREPRLQETAPAPLPAPPLGVRLAAVEELEVSIEK
                     LVAGGEGLARFEGAPIFVPRSAPGDRLRVRLVERHPDYGRAEIVEILEPGPDRRPDPH
                     PELSATGVADLQHLRDEVQPRLKAQAVWETLIHLGKLQMPEKVDLVTGEPWGYRLRTQ
                     LHTDRDPVTRGARVGYLARGTNDVVPVSGCALLVPELDELLSQIPAHLADGEPPWRID
                     LAAGEDRGVTVAPVIAGLPSGEVTTQVATASGAFTYSYDARCFFQGHRGLLPKLVEAV
                     VGPWSGEEAFDLYGGVGLFA"
     gene            complement(1592..2428)
                     /locus_tag="VEL74_23420"
     CDS             complement(1592..2428)
                     /locus_tag="VEL74_23420"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYG65552.1"
                     /translation="MKPVELRIGDLCITGWSRAGDETWFRVHPPGLALDTGRGAMQLA
                     GAQDVFLTHGHLDHALGVPYVLSQRSLHRLLHTRVFCPAETAGPLADLIAAAERLERA
                     RYRYELVPLRAGDRVEVGRDLAMEAFATDHVVPSLGFHLLRAKRRLAAGFAGLSGAEL
                     ADLRRRGVDTAEVVEEIWLSYPGDTGPAVFDLEPRLFGSRVLMLECTFLGEEKREHGE
                     RYKHMTLGDIVRRKDDFHNQALVLHHLSRRYKVEELRTEIERLLPELASRIHIMVEGE
                     AS"
     gene            complement(2477..3541)
                     /locus_tag="VEL74_23425"
     CDS             complement(2477..3541)
                     /locus_tag="VEL74_23425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65553.1"
                     /translation="MKKIVHVVGTGTIGEPLIGLFTDFRDKMGIDEVTFHKRTPLASD
                     KSKLNHLIARGAKLAVDEDVRAEFEKIGHEVTYDSREALERATVVVDCTPAGNDNKEK
                     IYESLSGPKGFLAQGSEFGFGKPYARGVNDEVLVPGEDRYIQIVSCNTHNITTLIKTV
                     CDDGNNELCLRKGTFVCMRRANDISQTSSYVPSPNVGKHDDPEFGTHHARDAHYVFET
                     LNKNLDLFSSATKLNTQLMHSIWFNLELERDMTLDEVKQRLKENPRVALTDKRFANLI
                     FSFGRDHGYYGRILSQTVVVTPTLMVRRKREVYGFCFTPQDGNSLLSSVSATLWLIDP
                     DWESVQTRLAPLRRWLYREI"
     gene            complement(3738..3995)
                     /locus_tag="VEL74_23430"
     CDS             complement(3738..3995)
                     /locus_tag="VEL74_23430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65554.1"
                     /translation="MSDGINIRNISIKCGNCNTYQTLSAFSRREGWNVYTYECENDTC
                     DPAVTRTLVEVPRDLDTFARRDPEWRGGGKHGEVGDGRPED"
     gene            4105..5121
                     /locus_tag="VEL74_23435"
     CDS             4105..5121
                     /locus_tag="VEL74_23435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG65555.1"
                     /translation="MRELSRTRTLLAVLLLTGLAGAVPPPGISQEEPAAEFGEQVSVG
                     YVLVPVIVRSGTGYARNLDQEDFKLLVDGRPARIESFERRAEAPASVVFLQDLSGSME
                     NAGKIEISREVVRFFLKTAVEGDEFSLATFAGGIRQVEVPFTGNRATLAEAVTGWEAY
                     GTTALHDAVAWVPEISLEGRNPKRFAILITDGVDNASQIPPEQAREIVRSAQLPVYVL
                     GLQPSDLPRAPKPAPAANGAAAPATEAEAQAQAVYRYADLLQELAASTGGRYYSISGP
                     DDLREVLTGIIKDFRHQYVLGFATGEGPSRYRALTVEVRGRNRPVLFRRGYQGPPPPS
                     PTGG"
     gene            5169..5774
                     /locus_tag="VEL74_23440"
     CDS             5169..5774
                     /locus_tag="VEL74_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYG65556.1"
                     /translation="MQNRLWTIAFVLILALATTGCATKKYVRQETGAVGARVDQVEGQ
                     VEETQTTVRQHDTRITEASRTAQEALQRAQEAGKLAQGKLLYETVLTDEKVKFGFDTA
                     DLSPEARGALDEFASQLKGENKGVYVEIQGHTDSVGSDKYNEELGLLRAESVRRYLNQ
                     EHQFPLHRINVISYGESAPIADNNSRQGRSQNRRVALVVLT"
     gene            complement(5849..6373)
                     /locus_tag="VEL74_23445"
     CDS             complement(5849..6373)
                     /locus_tag="VEL74_23445"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYG65557.1"
                     /translation="MKMKTLSRVRRSRPSALFALAAALLLLSCGGAGAGPEASGPRSA
                     DFRLKTLDGRTLGPKDFRGKVVVVDFWATWCVPCHVQTGILAPIHKEHGGKGLQILAA
                     NVGEEAATVRKFLEKKPLPYPVLMDPGEVSAELGVMALPAVMIIDKKGQVSFFQQGLV
                     DDSTLRRAIKQAGS"
     gene            complement(6483..>7780)
                     /locus_tag="VEL74_23450"
     CDS             complement(6483..>7780)
                     /locus_tag="VEL74_23450"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618737.1"
                     /note="catalyzes the reduction of ribonucleotides to
                     deoxyribonucleotides; the rate-limiting step in dNTP
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HYG65558.1"
                     /translation="EIPEQGVPADVLAAARSVYDDTLELGTKHGFRNAQVTVLAPTGT
                     IAFMMDCDTTGVEPDIALVKYKKLVGGGMIKIVNNTVPIALKRLGYDKDEVRAIVEYI
                     DEHDTIEGAPHLKEEHLPVFDCAFKPAQGARSIHYMGHLKMLAAVQPFISGAISKTIN
                     MPEESTPDEIMQAYIEGWQMGLKAVAIYRDNCKRSQPLSTKKEKGEEKVAAQAVQVVQ
                     AVTQAQVPLQERRAVRRKLPDERRAITHKFSINEHEGYLTVGLYDDGMPGEIFLVMAK
                     EGSTISGLMDSLATSISIALQYGVPLQTLVDKFSHTRFEPSGFTKNPEIPMAKSITDY
                     IFRWLASKFLSRESKAAAGVILRDEIPVAPAPAVPESLPLMGGGRLDTVAQAISVETQ
                     KVTFLYQQDAPSCSDCGSIMVRNGSCYKCLNCGTTSGCS"
BASE COUNT         1138 a         2757 c         2698 g         1187 t
ORIGIN      
        1 gcgaacagcc cgacgccgcc gtagaggtcg aacgcctcct ccccgctcca gggtccgacc
       61 accgcttcga cgagcttcgg cagcaggccg cggtgcccct ggaagaagca gcgggcgtcg
      121 taggagtacg tgaacgctcc cgacgccgtc gccacctggg tggtcacctc gcccgagggc
      181 agcccggcga tcaccggcgc cacggtcacg ccccggtcct cacccgccgc caggtcgata
      241 cgccaggggg gctcgccgtc cgcgaggtgc gcggggatct gggagaggag ctcgtcgagc
      301 tccggcacca gcagggcgca gccgctcacc ggcaccacgt cgttggtgcc gcgggcgaga
      361 tagccgacgc gcgcgccgcg ggtcaccggg tcgcggtcgg tgtggagctg cgtgcgcagc
      421 cgatagcccc agggctcgcc ggtgaccagg tcgaccttct cgggcatctg gagcttgccc
      481 aggtgaatca gggtctccca gaccgcctgc gccttgagcc gcggctggac ctcgtcgcgg
      541 aggtgctgca ggtcggcgac accggtggcg gagagctccg ggtggggatc gggccggcgg
      601 tccggccccg gctccaggat ctccacgatc tcggcgcggc cgtagtcggg gtgacgctcc
      661 accagccgga cccgcagccg gtcgccgggc gccgagcggg ggacgaagat cggcgccccc
      721 tcgaagcggg cgagcccttc gccgccggcg accagcttct cgatcgacac ctccagctcc
      781 tcgacggcgg cgagccgcac gcccagcggc ggcgcgggga gaggcgccgg cgcggtttcc
      841 tggagccggg gctcccgggg ttcccgagac tcccggtgcg gcggcacccg ccgccggtcg
      901 agccccggat cgcggtcatc ccgcgcccgc cactcgcgcc cctcggacct ccccggcccg
      961 ggccggcgct cgccgtcacg ggctcgccag tccggccggt ctcctcgtgg cccaccgggg
     1021 ccgcccgggc ctcggggacc gccagggccg cgtgggccgc caggccccct ggactccctg
     1081 gggccacccg ggccccgccg gtcgtcccgc tcccgccgtg gcccaccggg tccccggtcg
     1141 ggccggccct ggcgatccga gcgatcggac cgatccgacg gacctggccg atcaaaccgg
     1201 gccggccgcg ggccgccggg gccgcgccgc tcttcccgcg gccggtcctc tcgcgggcgc
     1261 tcttgccgcg gcccgggtcc ccgccgctcg ggctcgccac cgctcccctc cgccgccggc
     1321 cggcgccacc aggtcttggc ggcgtcgggc cggccctctc cccggcgggg ccccgggccc
     1381 ccctgccctc tcgggccgcc cggttctctc cgttcccccg gcccgccgcc cggccgcccc
     1441 cggccttcgg acggcccgcg gccctccccc ggtccccggc cggctcccgg cccccggcca
     1501 gctcccggtc cccggccgcc tcccggcccc cggcctccac cggcaccgcg gcccgcgccg
     1561 cgccctcccc caccgccgcc gccacttggt ctcatgaagc ctctccttcc accatgatgt
     1621 gaatccggga cgccaactcg ggcaggaggc gctcgatctc cgtgcgtagc tcctccacct
     1681 tgtagcggcg ggacaggtga tgcagcacca gtgcctgatt gtggaaatcg tccttcctcc
     1741 ggacgatgtc ccccagggtc atgtgcttgt agcgctcgcc gtgctccctt ttttcctctc
     1801 cgaggaaagt gcactcgagc atcagcacgc ggctgccgaa caggcgcggc tccaggtcga
     1861 agaccgccgg gccggtgtcg ccagggtagg agagccagat ctcctccacc acctccgcgg
     1921 tgtcgacgcc gcgccgacgc aggtcggcca gctcggcgcc ggacaggccg gcgaacccgg
     1981 cggccagacg gcgcttcgcg cgcagcaggt ggaacccgag gctcggcacc acgtgatcgg
     2041 tggcgaaggc ctccatcgcc aagtcgcgtc ccacctccac ccggtcgcca gcgcgcagcg
     2101 gcacgagctc ataacggtac cgcgcccgct ccaggcgctc ggccgccgcg atcaggtcgg
     2161 cgagcggccc ggccgtctcc gcgggacaga agacccgcgt gtggagcagc cggtggagcg
     2221 agcgttgcga caagacgtac ggaaccccca gcgcgtggtc caggtgcccg tgggtgagga
     2281 agacgtcctg ggcgccggcg agctgcatgg cgccgcgacc ggtgtccagg gcgagccccg
     2341 gcggatgcac gcggaaccag gtctcgtccc cggcgcgcga ccagccggtg atgcagaggt
     2401 ctccgatgcg cagctcgacg ggtttcatct caggggcggg accgtccccc gggtcgcccc
     2461 gggggacgag gccgggtcag atctcccggt acaaccagcg gcgcaacggc gccaggcggg
     2521 tctggacgct ctcccagtcc ggatcgatga gccagagggt ggcggagacc gacgagagca
     2581 gcgagttgcc gtcctgcggc gtgaaacaga agccgtagac ctcgcgcttg cggcgcacca
     2641 tcagcgtcgg cgtcaccacc acggtctgcg acaggatgcg gccgtagtag ccgtggtcgc
     2701 ggccgaagct gaagatgagg ttggcgaagc gcttgtcggt cagggccacc cgcgggttct
     2761 ccttgagccg ctgcttcacc tcgtccagcg tcatgtcgcg ctccagctcc aggttgaacc
     2821 agatcgagtg catgagctgg gtgttgagct tggtggcgct cgagaacagg tcgaggttct
     2881 tgttcagcgt ctcgaagacg taatgcgcgt cgcgggcgtg gtgggtgccg aactccgggt
     2941 cgtcgtgctt gccgacgttg ggcgacggca cgtagctcga ggtctgcgag atgtcgttcg
     3001 cccgccgcat gcagacgaag gtccccttgc gcaggcagag ctcgttgttg ccgtcgtcac
     3061 agaccgtctt gatcagggtg gtgatgttgt gggtgttgca cgagacgatc tggatgtagc
     3121 ggtcctcgcc cggcaccagc acctcgtcgt tgacgccgcg ggcgtacggc ttgccgaagc
     3181 cgaactcgct cccctgggcc aggaagccct tggggccgga gaggctctcg tagatctttt
     3241 ccttgttgtc gttgccggcc ggggtgcagt cgacgacgac ggtggcccgc tccagggcct
     3301 cccgcgagtc gtaggtgacc tcgtggccga tcttctcgaa ctcggcgcgc acgtcctcgt
     3361 cgaccgcaag cttggcgccg cgcgcgatca agtggttcag cttggacttg tccgaggcga
     3421 gcggggtccg cttgtggaac gtgacctcgt cgatccccat cttgtcccga aagtcggtga
     3481 acaggccgat cagcggctca ccgatcgttc ccgtgcccac gacgtggacg atcttcttca
     3541 tgatccctcg ctcaaagtaa tgggtggttc ggtacggcgg gtcgaaaccc ggtggagatt
     3601 agcacagcgc gtgccaggcc cgggggcctc gggagtccgc gggcccggcg caggcgactg
     3661 gcagcgtggt cggggggagg gggagcgcac acgaaggagc cgagcagggc ccgcggactc
     3721 cagaggcccc ccggccctca gtcctccggc cgcccgtccc ccacctcgcc atgcttgccg
     3781 ccaccccgcc actcggggtc gcggcgggcg aaggtgtcga gatcgcgcgg cacctcgacc
     3841 agcgtccggg tcaccgccgg gtcgcaggta tcgttctcgc attcgtaggt gtagacgttc
     3901 cagccctccc gccgggaaaa ggcggagagc gtctggtaag tgttgcagtt gccacacttt
     3961 atggagatgt ttcggatgtt gatcccgtcg ctcatcgagc ctcctcgcag gcccacccgg
     4021 gagtggaccg cgctccatca tcccatcagt ggcctgggga ttgcacatgc acgcttggct
     4081 ataaatgagt aggatatccg tccgatgaga gaactctccc gcactcgcac gctgctggcc
     4141 gtactcttgc ttacagggct cgccggagcc gtaccgccgc ccgggatctc ccaggaggag
     4201 ccagcggcgg agttcggtga gcaggtatcg gtcgggtacg tgctggtgcc cgtgatcgtc
     4261 cggtcgggca cgggctacgc caggaacttg gaccaggagg acttcaagct gctggtcgac
     4321 ggcaggccgg cccggatcga gagcttcgag cggcgggcgg aggcaccggc gagcgtcgtc
     4381 ttcctgcagg acctgtccgg gagcatggag aacgccggca agatcgagat cagccgcgag
     4441 gtggtgcggt tcttcctcaa gaccgccgtc gagggggacg agttctccct cgccaccttc
     4501 gcgggcggta tccgccaagt cgaggtgccg ttcaccggca accgcgccac tctggccgag
     4561 gccgtgacgg gttgggaggc ctacggcacc accgcgctcc acgacgccgt ggcctgggtg
     4621 ccggagatct cgctggaggg gcggaacccg aagcggttcg ccatcctgat caccgacggg
     4681 gtcgacaacg ccagccagat cccgcccgag caggcgcgcg agatcgtccg ctcggcgcag
     4741 ctcccggtct acgtcctggg cctgcaaccg agcgacctgc cccgcgcccc gaagccggcg
     4801 ccggcagcga acggcgcggc ggccccggcg accgaggcgg aggcccaggc gcaggcggtc
     4861 taccggtacg ccgacctact gcaggagctg gcggcgtcca ccggcggccg ctactactcg
     4921 atctccggcc ccgacgacct ccgggaggtg ctgacgggca tcatcaagga ttttcggcat
     4981 caatacgtgc tgggcttcgc gaccggtgaa gggccttcgc ggtaccgggc gctcaccgtc
     5041 gaagtccgcg gcaggaaccg cccggttctg ttccggcgcg gctaccaggg accgccgccc
     5101 ccctccccga cgggcggcta gccgggtccg ccacgaggcg ggccttcgag aaaacaggag
     5161 gaaacaccat gcagaacaga ctttggacca tcgcgttcgt gctgatcctg gcgctggcca
     5221 ccaccggctg cgccaccaag aagtacgtcc gccaggagac gggggcagtc ggcgcccggg
     5281 tggaccaggt ggaagggcag gtggaagaga cgcagaccac ggtccgccag cacgacaccc
     5341 ggatcaccga agcctcccgc accgcccagg aggcgctgca gcgggcccag gaggcgggca
     5401 agctcgccca gggcaagctc ctctacgaga ccgtgctcac cgacgagaag gtgaagttcg
     5461 gcttcgacac ggccgacctc tcgcccgagg cgcgcggggc tctggacgag ttcgccagcc
     5521 agctcaaggg tgagaacaag ggagtctacg tcgagatcca ggggcacacc gacagcgtcg
     5581 gctcggacaa gtacaacgag gagctggggc tgctgcgggc cgagtcggtc cggcgctacc
     5641 tgaaccagga gcaccagttc ccgctccacc gcatcaacgt catttcctac ggtgagtcgg
     5701 cgcccatcgc ggacaacaac agccgccagg gccgctccca gaaccggcgc gtggcgctgg
     5761 tcgtcctgac ctagcccctc ctcccgggtt ccccctctcc cggacgccgg gggaggggga
     5821 ggtccggccc tcccgcctca gaccggcctc aggatcctgc ctgcttgatc gcccggcgca
     5881 gggtggagtc gtccaccagc ccctgctgga agaagctgac ctgccccttt ttgtcgatga
     5941 tcatcacggc ggggagggcc atgaccccca gctcggcgga gacctcgccg ggatccatca
     6001 gcaccgggta gggcagcggc ttcttctcga gaaacttgcg gacggtcgcc gcttcttcgc
     6061 cgacattggc ggcgaggatc tgcagcccct tgccgccgtg ctccttgtgg atgggcgcga
     6121 gaatcccggt ctgcacgtga caggggacgc accaggtggc ccagaagtcg acgaccacca
     6181 ccttgccgcg gaagtccttg ggaccgagag tgcggccgtc cagggtcttc agccggaagt
     6241 cggcggacct gggaccgctc gcctccgggc cggctccggc gccgccgcag gagaggagca
     6301 ggagcgccgc cgccagggcg aacagggcgg acgggcggga acggcggaca cgggagagcg
     6361 tcttcatctt catgacctca caaggatagc aggagcgagt cccaccccga gaaacaaaga
     6421 ggggaggcgc ggtattcgcg cctccccgaa gagactccca cctggcgtgg cggacgtgcg
     6481 tcctagctgc agccgctcgt cgtgccgcag ttgaggcact tgtagcaact gccgttgcgc
     6541 accatgatcg agccgcagtc gctgcagctc ggggcgtcct gctgatagag gaaggtcacc
     6601 ttctgggtct cgacgctgat cgcctgcgcc accgtgtcga gccggccgcc gcccatcagc
     6661 ggcagcgact cggggaccgc cggagccggg gccacgggga tctcgtcgcg caggatcacc
     6721 cctgccgccg ccttgctctc ccgcgacagg aacttcgagg cgagccaccg gaagatgtag
     6781 tcggtgatcg acttcgccat cgggatctcg gggttcttgg tgaagcccga cggctcgaag
     6841 cgggtgtggc tgaacttgtc gaccagcgtc tgcagcggca ccccgtactg gagggcgatc
     6901 gagatcgagg tggcgagcga atccatgagg cccgagatgg tggacccctc cttcgccatc
     6961 acgaggaaga tctcgcccgg catgccgtcg tcgtagaggc cgaccgtcag gtacccctcg
     7021 tgctcgttaa tcgagaactt gtgggtgatc gcccggcgct cgtcggggag cttgcggcgc
     7081 accgcgcggc gctcctggag cggcacctgg gcctgcgtga ccgcttggac gacctgcacc
     7141 gcttgcgccg ccaccttctc ctcccccttc tccttcttgg tggagagcgg ctggctgcgc
     7201 ttgcagttgt cacggtagat ggcgaccgcc ttcaagccca tctgccagcc ttcaatgtag
     7261 gcctgcatga tctcgtccgg cgtcgactcc tcgggcatgt tgatggtctt gctgatggcg
     7321 cccgagatga acggctgcac cgcggccagc atcttgaggt gccccatgta atggatggag
     7381 cgcgccccct gcgccggctt gaaggcgcag tcgaagacag gcaggtgctc ctccttgagg
     7441 tggggagccc cctcgatggt gtcgtgctcg tcgatgtact cgacgatggc gcggacctcg
     7501 tccttgtcgt agcccagccg cttcaaggcg atcggcacgg tgttgttgac gatcttgatc
     7561 atgccgccgc cgaccagctt cttgtacttg acgagggcga tgtcgggctc gacgccggtg
     7621 gtgtcgcagt ccatcatgaa ggcgatcgtc ccggtggggg cgagcacggt cacctgagcg
     7681 ttgcggaagc cgtgcttcgt gccgagctcg agcgtgtcgt cgtagaccga ccttgccgcc
     7741 gcgaggacgt ccgcgggaac gccctgctcg gggatctcgt
//
LOCUS       DASPJI010000833         3922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_225454, whole genome shotgun sequence.
ACCESSION   DASPJI010000833 DASPJI010000000
VERSION     DASPJI010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225454"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VEL74_23455"
     CDS             <1..831
                     /locus_tag="VEL74_23455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HYG65559.1"
                     /translation="GPNGAGKTTTIKMLTGLVPPSGGRGRVAGFDVRTQAKEIKRRIG
                     YMSQLFSLYGDLTVGENIGFFSGLYGVPRPRRAERRDWVLRMAGLEGQRDRLTGDLSL
                     GWKQRLALGCAVLHEPPILFLDEPTSGVDPISRRSFWDLIYTLAEGGTTVFVTTHYME
                     EAEYCNRLALMNRGKLIALDSPEGLRRSRQRPLFDLRTSDGPAAADLLEDAPGVEQVG
                     LFGRNLHVEVADAAAARKDLPALLAARGIRVESMEEITPSLEDVFLSLVEEAGGAPVD
                     "
     gene            847..1971
                     /locus_tag="VEL74_23460"
     CDS             847..1971
                     /locus_tag="VEL74_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG65560.1"
                     /translation="MSLTRLLAMARKESIQLRRDPRSLLLAFALPVLLLILFGYAISW
                     DVGNIRTAVLDGDRSARSRELLDAFRASGYFTFVEILERPAEITPLLERSGAQVVLVI
                     PPGFADAVGAGRTVAVQAVVDGSDANTATIALAYAEAVTRTFSADFQDAVLRGATLQP
                     PVEAESRVWFNEELASRNMIVPGLVAVIMMIIAAMLTSLSIAREWERGTMEQLAATPV
                     ARTEVVLGKLLPYVVIGLLDVAVSSLLGVYLFDVPFRGSAALLMALSFFFLLGALGLG
                     MFISAVAKSQVLATQIAMVATFLPAFLLSGFMFSLETMPKALQAISYLIPARYFLVVT
                     RGIFLKGVGLEVLRFQGLLMIAFAALGLSLAVVKFRKELG"
     gene            1968..3077
                     /locus_tag="VEL74_23465"
     CDS             1968..3077
                     /locus_tag="VEL74_23465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG65561.1"
                     /translation="MSGERILEMVRKEFRQLFRDPRLRRIVFVAPLLQLIAFGYAVST
                     DVRDTSTFVVDGDRTAESRALVDALTASGYFRVVGGSERPADLMRALDHGDATMGVVI
                     PRGFSRDLSNRSARVQILLDGTNSNVATVARGYAERIVQSFALEQAGGASAVPVAVDL
                     RARAWYNPDLASRNYNVPAVIGAIILLVCLILTSLAIVREREIGTLEQLRVSPLTPME
                     LILGKTIPFALIGLVDLAVITAVALLWFGIPFRGSALVLLLGSVLYLLSGLGIGLFIS
                     SISRTQQEAFMAAFLVFMPAMLLSGFMFPVTSMPEVFQWMTLANPMRHYLEIVRGVFL
                     KGSGFEDLGRQFVTLTAMGAGILWLAASRFSRQAG"
     gene            complement(3103..>3922)
                     /locus_tag="VEL74_23470"
     CDS             complement(3103..>3922)
                     /locus_tag="VEL74_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HYG65562.1"
                     /translation="ANAIATTARNPVSGMTMLTIIISSVVLLRFGVSGTTGMFFVMAI
                     AGVVCTALSVSGQTITDLKAGYWLGSTPAVQERVKFLGIIVSAIAAALTIVILATAFQ
                     FGQAAPGDARVVLAAPQASIMQALVQGFMSQQPVPYILFATGALIALVLEMLGAPALI
                     FALGMYLPLELNTPALVGGFLAHYVDKRSNRIGGEEGRSIRERGVIIASGLMAGGALG
                     GVFGASLRLIPGYTEDWVRTPFFDNEPVSQLVSLALFVALCVYLWRASVRGRRA"
BASE COUNT          538 a         1405 c         1354 g          625 t
ORIGIN      
        1 ggccccaacg gcgcgggcaa gaccaccacc atcaagatgc tgaccgggct tgtcccgccg
       61 tcaggagggc gcggccgggt ggcgggcttc gacgtccgca cccaggcgaa ggagatcaag
      121 cgccgcatcg gctacatgtc gcagctcttc tccctctatg gcgacctgac cgtcggggag
      181 aacatcggct tcttctcggg cctctacggc gtgccgcggc cgcgccgcgc cgagcgccgc
      241 gactgggtgc tgcgcatggc cggcctggaa gggcagcgcg accggctcac cggcgatctg
      301 tccctcggct ggaagcagcg tctggccctc ggctgcgcgg tccttcacga gccccccatc
      361 ctcttcctgg acgagccgac ctccggggtg gacccgatct cgcggcgcag cttctgggac
      421 ctcatctaca ccctggccga gggggggacc acggtcttcg tcaccaccca ctacatggag
      481 gaggcggagt actgcaaccg cctcgcgctg atgaaccggg ggaagctgat cgccctggac
      541 agcccggagg ggctgcggcg gagccgccag cgcccgctct tcgacctgcg caccagcgac
      601 ggccccgccg ccgccgacct cctggaggac gcccccggcg tcgagcaggt cggcctcttc
      661 ggccgcaacc tccacgtcga ggtcgccgac gccgccgcgg cccgcaagga tctgccggcc
      721 ctcctcgccg cccgcggcat ccgggtggag tcgatggagg agatcacccc gtcgctcgag
      781 gatgtcttcc tctccctggt ggaggaggcc ggcggcgcgc cggtggattg aggaggagcc
      841 ccgcccatga gcctcacccg cctgctcgcc atggcccgca aggagtcgat ccagctccgg
      901 cgcgatccgc ggagcctgct gctcgccttc gcgctgccgg tcctgctgct gatcctcttc
      961 ggctacgcca tctcgtggga cgtggggaac atccgcacgg cggtgctgga cggcgaccgg
     1021 tcggcgcgca gccgcgagct cctcgacgcc ttccgcgcct cgggctactt caccttcgtc
     1081 gagatcctgg agcggccggc cgagatcacc cccctgctcg agcggagcgg cgcccaggtg
     1141 gtgctcgtca tcccgccggg cttcgcggac gctgtcgggg cggggcggac ggtggcggtc
     1201 caggcggtgg tcgacggcag cgacgccaac acggcgacca tcgccctcgc ctatgccgag
     1261 gcggtgacgc gcacgttctc cgccgatttc caggatgcgg tgctccgcgg cgccaccctc
     1321 cagccgccgg tcgaggccga gtcgcgggtc tggttcaatg aggagctggc gagccgcaac
     1381 atgatcgtgc cggggctggt ggcggtcatc atgatgatca tcgcggccat gctcacctcc
     1441 ctctccatcg cccgcgagtg ggagcgcggc accatggagc aactcgcggc cacgccggtc
     1501 gcccgcaccg aggtggtgct cggcaagctc ctcccctacg tcgtcatcgg cctcctggac
     1561 gtggcggtct cgtcgctcct cggggtctac ctcttcgacg tccccttccg cggcagcgcg
     1621 gcgctcctca tggccctctc cttcttcttc ctgctcggag cgctcggcct ggggatgttc
     1681 atctcggcgg tcgcgaagtc ccaggtgctg gcgacgcaga tcgcgatggt ggcgaccttc
     1741 ctgccggcgt tcctgctgtc ggggttcatg ttcagcctgg agaccatgcc gaaggcgctc
     1801 caggccatct cgtacctgat cccggcgcgc tacttcctgg tggtgacgcg ggggatcttc
     1861 ctcaaggggg tggggctcga ggtgctgcgc ttccaggggc tcctgatgat cgccttcgcc
     1921 gccctcgggc tgagcctcgc ggtggtcaag ttccggaagg agctgggatg agcggcgagc
     1981 ggatcctgga gatggtgcgc aaggagttcc ggcagctctt ccgcgacccc cgcctgcggc
     2041 gcatcgtctt cgtcgccccg ctgctccagc tcatcgcctt cggctacgcc gtctcgaccg
     2101 acgtccgcga caccagcacc ttcgttgtgg acggcgaccg gaccgccgag tcgcgggcgc
     2161 tggtggacgc cctcaccgcc tccggctact tccgggtcgt cggcggatcg gagcgcccgg
     2221 cggacctgat gcgcgccctg gaccacggcg acgccaccat gggcgtggtc atcccccggg
     2281 gcttctcccg cgatctctcg aaccggagcg cgcgggtgca gatcctcctc gacggtacca
     2341 actccaacgt ggcgaccgtc gcccgcggct acgccgagcg catcgtccag agcttcgccc
     2401 tggagcaggc cgggggcgcc tcggccgttc cggtggccgt cgacctgcgg gcgcgggcct
     2461 ggtacaaccc ggacctggcg agccgcaact acaacgtgcc ggcggtgatc ggcgcgatca
     2521 tcctgctggt ctgcctgatc ctgacctcgc tcgccatcgt ccgcgagcgc gagatcggca
     2581 ccctcgagca gctccgggtg agcccgctga cgccgatgga gctgatcctg ggcaagacca
     2641 tccccttcgc cctcatcggc ctggtggacc tggcggtgat caccgccgtg gcgctcctct
     2701 ggttcggcat cccgttccgg gggagcgccc tggtgctgct cctggggagc gtcctctatc
     2761 tattgtcggg gctggggatc ggcctcttca tctccagcat ctcgcgcacc cagcaggagg
     2821 ccttcatggc ggccttcctg gtgttcatgc cggcgatgct gctgtcgggc ttcatgttcc
     2881 cggtgaccag catgcccgag gtcttccagt ggatgaccct ggcgaacccg atgcggcact
     2941 acctggagat cgtgcgcggc gtcttcctga agggctcggg cttcgaggac ctggggaggc
     3001 agttcgtcac cctgaccgcg atgggcgccg gcatcctctg gctggccgcc tcccggttca
     3061 gccgccaggc ggggtgaggc cctcaccccg ccatggaccg ggctacgccc ggcgtccccg
     3121 caccgaggcc cgccacagat agacgcagag ggcgacgaag agggcgagtg agaccagctg
     3181 cgagaccggc tcgttgtcga agaacggagt gcggacccag tcctcggtgt agccggggat
     3241 gagcctcaga gaggcgccga agacgccgcc cagcgcgccg ccggccatga ggcccgaggc
     3301 gatgatgacg ccgcgctcgc ggatgctccg gccctcctcg ccgccgatcc ggttggagcg
     3361 cttgtcgacg tagtgggcga ggaagccgcc caccagggcc ggcgtgttga gctccagcgg
     3421 caggtacatg ccgagggcga agatcagcgc cggcgcgccg agcatctcca gcaccagggc
     3481 gatgagagcg ccggtggcga acaggatgta gggcaccggc tgctggctca tgaagccctg
     3541 gacgagggcc tgcatgatgg acgcctgggg cgccgcaagc accacccggg cgtcgccggg
     3601 cgccgcctgg ccgaactgga aggccgtcgc caggatcacg atggtcaggg ccgccgcgat
     3661 cgccgagacg atgatcccca ggaacttgac ccgctcctgc acggcggggg tcgagcccag
     3721 ccagtagccc gccttaaggt cggtgatcgt ctgccccgac accgacagcg ccgtgcagac
     3781 cacgccggcg atcgccatga cgaagaacat gccggtggtc cccgagacgc cgaagcgcag
     3841 cagtaccacg gacgagatga tgatggtgag catggtcatg cccgagaccg ggttgcgcgc
     3901 cgtggtggcg atggcgttgg cg
//
LOCUS       DASPJI010000834         4707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_225483, whole genome shotgun sequence.
ACCESSION   DASPJI010000834 DASPJI010000000
VERSION     DASPJI010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225483"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /gene="metG"
                     /locus_tag="VEL74_23475"
     CDS             <1..1601
                     /gene="metG"
                     /locus_tag="VEL74_23475"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HYG65563.1"
                     /translation="WYCSQCEAFYTEKELGPDKSCPVHGTPVEWKSEENVFFRLSKYQ
                     QPLLDWYKDPASVRPASRLNEVRSFVEAGLRDLSVSRANLTWGIPFPGKQGQTVYVWL
                     DALTNYISALGFGGQESGLYGSFWQQGDVRLHLVGKDILRFHAVYWPAFLMSAGLPLP
                     TSVWAHGWWLRDGRKMSKSAGNVVRPDELIERFGADSLRYFMLREMVFGQDASFSDEA
                     FIDRYNSDLANDLGNTVSRLVTLSRRAFDGKTPPIPCGVNLLADASGPAVRDYHAAMK
                     DFAFQRALESLWRLLAETNQYIVTREPWALIKNEGDSDKLARILWNGLEATRIVATGL
                     LPFMPEAATRVLAAIGIQEPPRSLDALQWGGLPVSVAIPEPKPVFPRIDKEAYLGSAE
                     AAPALAAEPKPAKKKQEKAPQQKAPEKEKGTGMIAIDRFMETELKVATVQAAERVPKS
                     DKLLKLTVDVGEEGGPRTVVAGIGLAYQPEDLVGKQVVVVANLQPAKLMGIESQGMVL
                     AASLDGKPVLLHPAAEVPNGTRVR"
     gene            1608..2384
                     /locus_tag="VEL74_23480"
     CDS             1608..2384
                     /locus_tag="VEL74_23480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HYG65564.1"
                     /translation="MTALVDSHCHLQSLPPDERERALDNARERGVRGFLVPATRLDEA
                     DDILAFCHRHPDVWSALGVHPHEASSWQEGDRERLAGLLSDPRAVAVGECGLDFFYDH
                     SPRDVQFRVLREQWSLAIELGLPVVVHNRDSNDEMLEVVREPEFAHLQADFHSFAGGL
                     AMARELIGRGFYLGLSGMITFPKADNVREVIPILPPDRCLVETDTPYLAPVPHRGKPN
                     RPAYVVEVAARLAQETGETPKQVGVRTGENFFRLFAKAAA"
     gene            complement(2392..3159)
                     /locus_tag="VEL74_23485"
     CDS             complement(2392..3159)
                     /locus_tag="VEL74_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HYG65565.1"
                     /translation="MAATVDRSALVAYLDQYLDAARGRDYGPNGLQVEGKADIRKVVT
                     GVSACHELFVRAREAGADAVLVHHGLFWDGMPRVLTGVQHRRVAELIRADINLLAYHL
                     PLDRHAEVGNNVIACRDFGLVDLQPFGFHEGLPIGFQGRFPEPVSAAELLDRCRRVYG
                     QEPLAFLEGPDPLSSLGIISGGAQRDFYSAISGGLDAYITGEVSEWVMNVAREARVHY
                     LAAGHYATERLGIRALGEHLRERFGVDVEFIDVPNPV"
     gene            complement(3232..>4707)
                     /gene="pnp"
                     /locus_tag="VEL74_23490"
     CDS             complement(3232..>4707)
                     /gene="pnp"
                     /locus_tag="VEL74_23490"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HYG65566.1"
                     /translation="IAKLCPEGDENAPSATLVGSVFHDLEAKIVRWNILDDGIRIDGR
                     DVKTVRPILSEVGVLPRTHGSALFTRGETQALASATLGTSRDAQVIEEYEGESQQKFL
                     LHYNFPPFSVGEVKFMRAPGRREIGHGVLARRALMPVLPHEDEFPYTVRVVSDILESN
                     GSSSMATVCGGSLCLFDAGVPLLAPVAGVAMGLIKGDDNFAVLSDIAGQEDHYGDMDF
                     KVAGTRSGITALQMDIKITGVTREIMQQALRQAQAGRFHILDIMERVIPQPRTDISRF
                     APRLYTLQISKEKIRDIIGPGGKTIRSIIDETGCNIEVEDDGRVIIASPDEPAARRAI
                     QMIERLTEVPEVGKLYTGQVRRVEPYGAFVEIMPGTDGLVHISELAPYRVREVTDLVK
                     EGDEMTVKVISIDETGRVRLSRKAVIMESPDYDPAQYEGLEMVPVGAGNGGGERSERG
                     GDRGGRGGDRGGRGGDRGGRGGDRGRNGGGGGRGGDRGPRR"
BASE COUNT          724 a         1638 c         1617 g          728 t
ORIGIN      
        1 ggtggtactg ctcgcagtgc gaggcgttct acaccgagaa ggagctgggg ccggacaaga
       61 gctgcccggt gcacggcacg cccgtcgaat ggaagtcgga ggagaacgtc ttcttccggc
      121 tgtccaagta ccagcagccg ctcctcgact ggtacaagga cccggcgagc gtccgcccgg
      181 cgtcgcgcct caacgaggtc cgctcgttcg tcgaggccgg gctccgcgac ctctcggtct
      241 cgcgcgccaa cctgacctgg ggtatcccgt tccccggcaa gcaggggcag acggtctacg
      301 tctggctcga cgccctcacc aactacatca gcgccctcgg gttcggtggc caggaaagtg
      361 gcctctacgg cagcttctgg cagcaggggg atgtccggct ccacctggtg ggcaaggaca
      421 tcctgcgctt ccacgccgtc tactggcccg ccttcctgat gtccgccggc ctgccgctgc
      481 ccaccagtgt ctgggcgcat ggctggtggc ttcgtgacgg ccgcaagatg tccaagtcgg
      541 ccggcaacgt ggtccggccc gacgagctga tcgagcgctt cggcgccgac agcctgcgct
      601 acttcatgct gcgcgagatg gtcttcggcc aggatgccag cttctcggac gaggcgttca
      661 tcgaccgcta caacagcgac ctggcgaacg acctgggcaa cacggtcagc cggctggtga
      721 ccctgtcacg ccgcgccttc gacggcaaga ccccgcccat tccttgcggc gtcaacctgc
      781 tggccgacgc gtcgggtccg gcggtgcggg actaccacgc ggcgatgaag gacttcgcct
      841 tccagcgcgc gctcgagagc ctctggcgcc tgctcgctga gacgaaccag tacatcgtca
      901 cgcgagagcc gtgggccctc atcaagaacg aaggggattc ggacaagctg gcgcgcatcc
      961 tgtggaacgg gctcgaggca acccgcatcg ttgccaccgg tctgctgccg ttcatgcccg
     1021 aggccgcgac ccgggtgttg gcggccatcg gcatccagga gccgccgcgg agcctcgacg
     1081 cgctgcaatg gggcgggctg cctgtctcgg tggcgatccc ggagcccaag ccggtgttcc
     1141 cgcggatcga caaggaagcc tacctgggga gcgccgaggc cgcgccggca ctggccgccg
     1201 agccgaagcc cgcgaagaag aagcaggaga aggctccaca gcagaaagct ccagagaaag
     1261 agaaagggac ggggatgatc gcgatcgatc gcttcatgga gaccgagctc aaagtggcca
     1321 ccgtccaggc ggccgagcgg gtccccaagt cggacaagct gttgaagcta accgtggacg
     1381 tgggggagga ggggggccct cgcaccgtcg tcgccggtat cgggctggcg tatcagcccg
     1441 aggatctggt gggcaagcag gtcgtggtgg tggcgaacct ccagccggcc aagctcatgg
     1501 ggatcgagtc ccagggcatg gtcctcgccg catccctcga tggcaagccc gtgctgctgc
     1561 atccggccgc ggaggtcccg aacgggaccc gtgtgcggtg agctgcggtg accgccctcg
     1621 tcgactccca ctgtcatctg cagagtctgc ccccggacga gcgcgagcgg gccctggaca
     1681 acgcccgcga gcgcggggtc cgcggcttcc tggtgccggc gacgcggctg gacgaggccg
     1741 acgacatcct ggcgttctgc caccgtcacc cggacgtctg gagtgccctc ggggtccatc
     1801 cccacgaggc gtcgtcctgg caggaagggg accgggagcg cctggccggg ctcctttccg
     1861 atccccgggc cgtggcggtg ggggagtgcg gcctcgactt cttctatgac cactcgccgc
     1921 gcgacgtgca gttccgggtg ctccgcgagc agtggagcct ggccatcgag ctcgggctgc
     1981 cggtcgtggt tcacaaccgc gacagcaacg acgagatgct ggaggtggtg cgcgagccgg
     2041 agttcgccca tctccaggcg gatttccact ccttcgccgg cggactggcc atggcccggg
     2101 agctcatcgg ccgcggcttc taccttgggc tttcggggat gatcaccttc ccgaaggccg
     2161 acaacgtccg cgaggtcatc cccatcctgc ccccggaccg ctgcctggtg gagaccgata
     2221 ccccctatct cgcgccggtg ccgcaccggg gcaagcccaa ccgtcccgcc tacgtggtgg
     2281 aggtggccgc ccggctcgcg caggagaccg gagagacgcc taagcaggtg ggggtccgca
     2341 cgggggagaa cttcttccgg ctgttcgcga aggcggcggc ctgaccctcg ctcagacggg
     2401 gttcggcacg tcgatgaact cgacgtcgac gccgaaccgc tcgcgcagat gctcgccgag
     2461 ggcgcggatg cccagacgct ccgtggcgta gtggccggcg gccaggtagt gcacccgggc
     2521 ctcccgcgcc acgttcatta cccactcgct cacctcgccc gtgatgtagg cgtcgaggcc
     2581 gcccgagatg gcggagtaga agtcgcgttg cgcgccgccg ctgatgatcc ccagggacga
     2641 caacgggtcc ggcccttcca ggaaggcgag cggctcctga ccatagaccc gccggcagcg
     2701 gtcgagcagc tcggcggcgg agaccggctc ggggaagcgc ccctggaagc cgatgggcag
     2761 gccctcgtgg aacccgaacg gctgcaggtc gacgaggccg aagtcgcggc aggcgatgac
     2821 attgttgccc acctcggcgt gccggtccag cggcaggtga taggccagca ggttgatgtc
     2881 ggcccggatc agctcggcca cccgccggtg ctgcaccccg gtgagcaccc ggggcattcc
     2941 gtcccagaac agcccgtgat gcaccaggac cgcgtccgcc cccgcctcgc gggcgcggac
     3001 gaacagctcg tgacaggcgg agacgccggt cacgacctta cggatgtccg ccttaccctc
     3061 cacctggagg ccgttggggc cgtagtcgcg cccgcgggcg gcgtcgaggt actggtccag
     3121 gtaggcgacg agagcggagc gatcgacggt ggcagccatg gggaggagtc taacggaaaa
     3181 gaaaaagggg ggcgcgagcc cccctttttc ctttcggtgt caggcgtgag cctagcggcg
     3241 tgggccgcgg tctccgccac ggccaccgcc accgccgttg cggccacggt caccaccgcg
     3301 gccaccccgg tcgccacctc ggccgccgcg gtctccgccc cggccgccgc ggtctccgcc
     3361 gcgctcgctc cgctcgccgc cgccgttgcc ggcgcccacg ggcaccatct ccaacccctc
     3421 gtactgcgcg gggtcgtagt ccggcgactc catgatcacg gccttgcgcg acaggcgcac
     3481 ccggccggtc tcgtcgatgc tgatgacctt cacggtcatc tcgtccccct ccttcacgag
     3541 gtcggtgacc tcgcggacgc ggtagggggc gagctcgctg atgtgcacca gcccgtcggt
     3601 gcccggcatg atctcgacaa aggctccgta gggctcgacc cgccggactt ggccggtgta
     3661 gagcttgccg acctccggca cctcggtcag gcgctcgatc atctggatcg cccgccgcgc
     3721 cgccggctcg tccggcgacg cgatgatgac ccggccgtcg tcctcgacct cgatgttgca
     3781 gccggtctcg tcgatgatcg agcggatcgt cttgccaccg ggtccgatga tgtcgcggat
     3841 cttctccttc gagatctgca gcgtgtacag gcgcggcgcg aacctggaga tgtccgtccg
     3901 cggctgagga atcacccgct ccatgatgtc caggatgtgg aagcgcccgg cctgggcctg
     3961 acgcagggcc tgctgcatga tctcccgggt cacgccggtg atcttgatgt ccatctggag
     4021 cgcggtgatg ccgctgcgcg tgcccgccac cttgaagtcc atgtcgccgt agtggtcttc
     4081 ctggccggcg atgtcggaga gcaccgcgaa gttgtcgtcg cccttgatga ggcccatcgc
     4141 cacgccggcg accggcgcca gcagcggcac gccggcgtcg aacaggcaga gcgagccgcc
     4201 gcacaccgtg gccatggacg aggagccgtt ggactccagg atgtccgaga ccacgcgcac
     4261 ggtgtagggg aactcgtcct cgtgcggcag caccggcatc agcgcccggc gcgccagcac
     4321 gccgtggccg atctcacgcc ggcccggcgc ccgcatgaac ttcacctcgc cgaccgagaa
     4381 cggcgggaag ttgtagtgaa gcaggaactt ctgctgactc tccccctcgt actcctcgat
     4441 cacctgggcg tcgcggctgg tccccagcgt cgccgaggcc agagcctggg tctcgccgcg
     4501 ggtgaacagg gccgagccgt gcgtgcgcgg cagaacgccg acttccgaca ggatagggcg
     4561 caccgtcttc acatcgcggc cgtcgatgcg gatgccgtcg tcgagaatgt tccagcgcac
     4621 gatcttggct tcgagatcgt ggaacaccga gccgaccagc gtcgccgaag gcgcgttctc
     4681 gtcgccctcg gggcagagct tggcgat
//
LOCUS       DASPJI010000835         7137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_567137, whole genome shotgun sequence.
ACCESSION   DASPJI010000835 DASPJI010000000
VERSION     DASPJI010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7137)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567137"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..3664)
                     /gene="dnaE"
                     /locus_tag="VEL74_23495"
     CDS             complement(185..3664)
                     /gene="dnaE"
                     /locus_tag="VEL74_23495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HYG65567.1"
                     /translation="MSDFVHLHLHSQFSLLDGANRLDDVINAAVQAGMPAVALTDHGN
                     MFGAIEFYEKARKAGIKPIIGVEAYVAQGSRLDRTPGKGSSNHLVLLAANETGYRNLI
                     KLTSSAYLEGFYYKPRIDKELLRKHSEGIICLSACLKGEVNEKIVANQEKEAEAAARD
                     FQDIFGEGNFYLEMQDHGIPEQRLAADVTRRIAKRNGIPLVVSNDAHYLRKDDAFAHD
                     VLLCIGTQKALSDPDRMRYASDNFYVKSAAEMHQLFPNDHDAIENTLAIAERCDLKIP
                     MGTFHLPEFPVPEGYTFLSYFQKVAREGLDERLAELRARGARRLAKNPEERYRERLEY
                     EIQVIEKMGFAGYFLVVWDFIRYAREHDVPVGPGRGSAAGSVVSWAMRITNIDPLQYD
                     LLFERFLNPERISMPDIDIDFCMRRRGEVIHYVGEKYGRDRVAQIITFGTLAAKAVIR
                     DVGRVMGIPYVKVDRIAKLVPDMTKSLAEAAKEVDALAAEVQKDPEVKKIVEVGSRLE
                     GLTRHASVHAAGVVITPRPLDDLVPLYKVTKGDEEQIMTQWDMTIIEKLGLLKMDFLG
                     LRTLTVIDDAVKILRLQGIEIDLDNLPLDDPEVFRLFCEGRTSGIFQFESRGMTDLLR
                     RAKPEKFEDLAAFNALYRPGALSVGMVEEYIQRKLGRKKIKYILPETQTILEETYGVI
                     VYQEQVMLIAVAVAGFTMAEADTLRKAMGKKKADVMAQMKEKFVSGGAARGVPREKSS
                     ELWDYIEPFAGYGFNKSHSVAYAMLAYQSAYLKSHYPVAFMAAMLNSELSSSDAIAKY
                     TAECRNMGIGILPPDINESSYFFTVVGDKIRYGLGAVKGVGEGAIESLLDARSRVGRF
                     HSLAQLACEVDLRSANRKVMECLVKGGAFDALGLHRASLWTSLDRILEYAQRRRHERE
                     MGQGSLFGGGGGLSVPEPEPDASTPPWPERERVRYEKEALGFYLTGNPLSEHQEALER
                     LVTHTTSTLKEGAEGSVTVGGLVTSFNRVKIKTGPNAGKFMGRFVLEDLEGGVPVTLF
                     SNQLQQFGHLLSEEALVLIKGQVRERGSEIELSVEEVIPLEAAAAKPLSGVELRLPPS
                     VTTGAMLKLRDLLIEHPGNVPVTLQVPLPDRTIRIATQDSLRIDLRPELAASIEGLLG
                     QGAIRELYPQPQA"
     gene            complement(3740..4300)
                     /locus_tag="VEL74_23500"
     CDS             complement(3740..4300)
                     /locus_tag="VEL74_23500"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HYG65568.1"
                     /translation="MSLTIPNLLSLLRMGLVPVFIIAVLDGEMRKALLVFAVAGVTDA
                     LDGFIARFWHQQSLLGAYLDPIADKLLLTSAYISLSIPTLNHGLLIPVWITVLVIARD
                     VLLVIVALVMYLAVGVRSFPPTILGKITTVSQVAAVVLVLLAGPFPNVETVALGVLYL
                     MTGLTVASGLNYVFRSNRLIESRPRA"
     gene            complement(4297..5646)
                     /locus_tag="VEL74_23505"
     CDS             complement(4297..5646)
                     /locus_tag="VEL74_23505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65569.1"
                     /translation="MLRLAAVPLLWAALLSACATTPPATSPREPVQSRPEPLAIEREE
                     LAYLVSPMEGYPLDVDAGRRERVENAYRDLLDAGATGEARATAAELLDTDPAFHPAQV
                     LAAQVDFAAGEHAAVARRLLAVGDQLPGYTASQLLLGRAAEKLGDIPLAYAAFRIVAP
                     RSSLAFQRTGELHPRALEIVVNRVNDALRRQSLEEAQSQLGLLREWAPSEMQTLEAEV
                     AVAVARSDRPAELSAVKALAARRPDDRKLLERRAELELAVGDPSAGLQIVQDLARRNP
                     NDPALAEKLAAAKFRWRLSLLPAAVREVAAKPDLDKADLALLLYWLVPNVHYGRPSAG
                     RIATDVLDHPHQEAIVRVVNLGLMDVDSLHRFGPSAPVRRGTAIRSLARLLARFAPKA
                     ACLGGAAIPDPSPSASCDVAARCRLVATAEECQPAAPLSGGDAVELIRRTLEHLGGS"
     gene            complement(5684..5899)
                     /locus_tag="VEL74_23510"
     CDS             complement(5684..5899)
                     /locus_tag="VEL74_23510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020756045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HYG65570.1"
                     /translation="MAKHNINIQDGYLFQSLKEGQQMFLELVTGRHLLGRLKRFDRFA
                     VVLESDGKEILIYKHAIATIAAAPPAA"
     gene            complement(5892..6842)
                     /gene="miaA"
                     /locus_tag="VEL74_23515"
     CDS             complement(5892..6842)
                     /gene="miaA"
                     /locus_tag="VEL74_23515"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HYG65571.1"
                     /translation="MTSPVPTVAITGATATGKSALALALAERLGGEIVNADALQAYRG
                     LDIGTAKPSPDERRRVPHHLVDILEPDERYSAGEFARRAAEAIADIRGRGRLPIVVGG
                     SGLYLRALFEGISPVPSGDPETREALRRRLEEEGLPALVAELRRLDPATAARLAPGDT
                     QRVLRALEVAQVSGKPLSTWITQQPFGTQRIAARRVGLTLPRAILYDRIARRVARMVQ
                     GGWVEEVAGLLAQGVSPGVPAFQAIGYRQIVRHVQGEWSLEQAVGETVRETRRFAKRQ
                     ETWFRKEPDIAWFPAQNDLEDYFSDVFEHVKQSGLGRAYG"
     gene            complement(6948..>7137)
                     /locus_tag="VEL74_23520"
     CDS             complement(6948..>7137)
                     /locus_tag="VEL74_23520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018528835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine beta-hydroxylase,
                     Fe(II)/alpha-ketoglutarate-dependent"
                     /protein_id="HYG65572.1"
                     /translation="LKDVVLEPGEICFIDNFKAVHGRRAFRARYDGEDRWLKRVNIVR
                     DLRKSRAVRPSAASRTIF"
BASE COUNT         1073 a         2341 c         2596 g         1127 t
ORIGIN      
        1 acggtcggcg agctgacgcc gcagcgcctg attggcacgc tccagctcgg cgatcctgtc
       61 gccggacgac acctgctcgt aaggctttga cacggctgag cctatctccg tcctgtggat
      121 acaccggttc aggagccagc attgctgcaa acggcacgcc aggcgcggcc cgtccggcgg
      181 acggtcaggc ctggggctgc gggtacagct cgcggatggc cccctgcccg aggagcccct
      241 cgatggaggc ggccagctcc ggccgcaggt cgatccgcag gctgtcctgg gtggcgatgc
      301 ggatggtgcg gtccggcagc gggacctgca gagtgaccgg cacgttgccg gggtgctcga
      361 tcagcaggtc gcggagcttg agcatggcgc cggtcgtgac cgaggggggc agccgcagct
      421 cgacgccgga gagcggcttg gcggccgcgg cctcgagcgg gatgacctcc tcgacggaca
      481 gctcgatctc gctcccccgc tcgcgcacct gccccttgat cagcaccagc gcctcttccg
      541 agagcagatg cccgaactgc tggagctggt ttgagaacag ggtcacgggc acgcccccct
      601 cgaggtcctc gagcacgaag cggcccatga acttgccggc gttggggccg gtcttgatct
      661 tgacgcggtt gaagctggtg accaggccgc cgacggtgac cgagccctcg gccccctcct
      721 tgagcgtcga ggtggtgtgg gtgaccaggc gctcgagcgc ctcctggtgc tcggagagcg
      781 ggttgccggt gagatagaag ccgagcgcct ccttctcata gcgcacccgc tcgcgctccg
      841 gccagggcgg agtcgaggcg tcgggctctg gctcgggcac cgacaggccg ccgccgccgc
      901 cgaacaggct cccctgcccc atctcgcgct cgtgccggcg gcgctgcgcg tactcgagga
      961 tgcggtcgag ggaggtccag agggaggcgc ggtgcaaccc cagagcgtcg aaggcgcccc
     1021 ccttgaccag gcactccatc accttgcggt tggccgagcg gaggtcgacc tcgcaggcga
     1081 gctgcgccag gctgtggaag cgaccgacgc ggctccgggc gtcgagcagc gactcgatgg
     1141 cgccctcgcc cacccccttg accgcgccga gcccgtagcg gatcttgtcc cctaccacgg
     1201 tgaagaagta gctgctctcg ttgatgtcgg ggggcaggat gccgatcccc atgttgcggc
     1261 actcggccgt gtacttggcg atcgcgtcgg aggacgagag ctcggagttg agcatcgccg
     1321 ccatgaacgc caccgggtag tggctcttga ggtaggcgct ctggtaggcg agcatggcgt
     1381 aggccacgct gtggctcttg ttgaagccgt agcccgcgaa gggctcgatg tagtcccaga
     1441 gctcggagga cttctcccgc ggcacgccgc gcgcggcgcc gccggagacg aacttctcct
     1501 tcatctgggc catgacgtcg gccttcttct tgcccatcgc cttgcgcagc gtgtcggcct
     1561 cggccatggt gaagccggcg acggccacgg cgatgagcat gacctgctcc tggtagacga
     1621 tgacgccgta ggtctcctcg aggatcgtct gcgtctcggg caggatgtac ttgatcttct
     1681 tgcggccgag cttcctctgg atgtactcct cgaccatgcc gaccgagagc gcgccgggcc
     1741 ggtagagggc gttgaaggcg gcgaggtcct cgaacttctc gggcttggcg cgccgcagca
     1801 ggtcggtcat gccgcgcgac tcgaactgga agatgccgct ggtgcgcccc tcgcagaaca
     1861 gcctgaagac ctcggggtcg tcgagcggga ggttgtcgag gtcgatctcg atcccctgaa
     1921 ggcgcaggat cttgaccgcg tcgtcgatca ccgtgagggt gcgcaggccc aggaagtcca
     1981 ttttgagaag gccgagcttc tcgatgatgg tcatgtccca ctgggtcatg atctgctctt
     2041 cgtccccctt ggtgaccttg tagagcggta ccaggtcgtc caggggacgg ggggtgatca
     2101 cgacgccggc ggcgtgcacc gaggcgtggc gggtgagccc ttcgaggcgg ctgcccacct
     2161 cgacgatctt cttgacctcg gggtccttct gcacctcggc ggcgagggcg tcgacctcct
     2221 tggccgcctc ggcgagcgac ttggtcatgt cggggaccag cttggcgatg cggtccacct
     2281 tgacgtaggg gatgcccatg acccggccga cgtcgcggat gaccgccttg gccgccaggg
     2341 tcccgaaggt gatgatctgc gccacgcggt cgcggccgta cttctcgccg acgtagtgga
     2401 tgacctcgcc ccggcgccgc atgcagaaat cgatgtcgat gtcgggcatg ctgatgcgct
     2461 cggggttgag gaagcgctcg aagagcaggt cgtactgcag cgggtcgatg ttggtgatgc
     2521 gcatcgccca ggagacgacc gagcccgccg ccgagccgcg gccggggccc accggcacgt
     2581 cgtgctcgcg ggcatagcgg atgaagtccc agaccaccag gaagtagccg gcgaagccca
     2641 tcttctcgat gacctggatc tcgtattcga gccgctcgcg gtagcgctcc tccgggttct
     2701 tggccaggcg cctggcgccg cgggcgcgca gctccgccag ccgctcgtcc agcccctcgc
     2761 gcgccacctt ctggaagtag gacaggaagg tgtacccctc cggcaccggg aactcgggga
     2821 gatggaaggt ccccatgggg atcttcaggt cgcagcgctc ggcgatggcg agggtgttct
     2881 cgatcgcgtc gtggtcgttc gggaagagct ggtgcatctc cgccgccgac ttgacgtaga
     2941 agttgtccga cgcgtagcgc atgcggtcgg ggtcggacag tgccttctgg gtgccgatgc
     3001 agagcaggac gtcatgggcg aacgcgtcgt ccttgcgcag gtagtgggcg tcgttcgaca
     3061 ccacgagcgg gatgccgttg cgtttcgcga tccgccgcgt cacgtcggcg gccagccgct
     3121 gctccgggat gccgtggtcc tgcatctcga gatagaagtt gccctcgccg aagatgtcct
     3181 ggaagtcgcg ggccgcggcc tcggcctcct tctcctggtt ggcgacgatc ttctcgttga
     3241 cctccccctt gaggcaggcc gacaggcaga tgatcccctc gctgtgcttg cgcagcagct
     3301 ccttgtcgat ccgcggcttg tagtagaagc cttcgaggta ggcgctggag gtcagtttga
     3361 tcaggttgcg gtagccggtc tcgttggcgg cgagcagcac gaggtggttg ctgctcccct
     3421 tgccgggggt gcggtcgagc cggctcccct gggcgacgta ggcctcgacg ccgatgatcg
     3481 gcttgatccc cgccttgcgg gccttctcgt agaactcgat ggcgccgaac atgttgccgt
     3541 ggtcggtcag ggccacggcg ggcatgccgg cctggacggc cgcattgatg acgtcgtcga
     3601 ggcggttggc gccgtcgagc aggctgaact ggctgtggag gtgcaggtga acgaagtcgg
     3661 acatcccagg caatggtacc agaccccctc aggcctccgg aggacgcagg acggcggtac
     3721 gccgggtcag gccgcccctt caggcgcggg ggcgcgactc gatcagccgg ttgctgcgga
     3781 agacgtagtt gaggccggag gcgacggtga ggcccgtcat cagatagagc acgcccagtg
     3841 ccacggtctc gacgtttggg aaggggccgg cgagcaggac caggacgacc gcggccacct
     3901 gggagacggt cgtgatcttc cccaagatcg tcggcgggaa gctgcggacg cccaccgcca
     3961 ggtacatcac cagggcgacg atcaccagca ggacatcgcg ggcgatgacc aacacggtga
     4021 tccagaccgg gatcagcagc ccgtggttga gcgtcgggat ggagagggag atgtaggccg
     4081 aggtgagcag cagcttgtcg gcgatggggt cgaggtaggc gccgagcagc gactgctggt
     4141 gccagaagcg agcgatgaag ccgtccaggg cgtcggtgac gccggccacc gcgaaaacca
     4201 gcagggcctt gcgcatctcg ccgtcgagga cggcgatgat gaacaccggc accaggccca
     4261 tgcgcagcag ggacagcagg ttggggatgg tgaggctcat gagccgccga ggtgctcgag
     4321 ggtgcggcgg atgagctcga ccgcgtcccc accggacagc ggcgccgccg gctggcactc
     4381 ctcggccgtg gcgacgaggc ggcagcgcgc ggccacgtcg caggacgccg atggcgaggg
     4441 atcggggatg gcggcgcctc ccaggcaggc ggctttcggg gcgaagcggg cgaggagccg
     4501 ggcgagcgag cgtatcgcgg tcccccgccg caccggcgcc gacggaccga agcggtgcag
     4561 cgagtccacg tccatgaggc ccagattcac cacgcgcacg atcgcctcct ggtgcgggtg
     4621 gtccagcacg tcggtggcga tgcggccggc cgacggacgg ccgtagtgga cgttgggcac
     4681 cagccagtag aggaggaggg cgaggtcggc cttgtccagg tcgggcttgg cggccacctc
     4741 gcggacggcg gccggcagca gcgacagccg ccacctgaac ttggcggcgg cgagcttctc
     4801 ggccagcgcc gggtcgttgg ggttgcggcg cgccaggtcc tggacgatct gcaggccggc
     4861 gctggggtcg ccgacggcga gctcgagctc ggcccggcgc tccagcagct tgcggtcgtc
     4921 ggggcggcgg gcggccagcg ccttgacggc gctgagctcc gccggccggt cgctccgcgc
     4981 cacggccacg gccacctcgg cctccagcgt ctgcatctcc gacggcgccc actcgcgcag
     5041 cagcccgagc tggctctgcg cctcctcgag gctctggcgg cgcaaggcgt cgttcacccg
     5101 gttgacgacg atctccagcg cccgggggtg gagctcgccg gtgcgctgga aagccaggga
     5161 gctacgggga gcgacgatac ggaaggcggc gtaggccagg gggatgtccc ccagcttctc
     5221 ggcggcgcgg ccgagcagga gctgggaagc ggtgtagccg gggagctggt cgccgacggc
     5281 gaggagccgg cgcgccaccg ccgcgtgctc gccggcggcg aagtccacct gcgccgccag
     5341 cacctgggcg gggtggaacg ccgggtcggt gtcgagcagc tcggcggccg tggcacgggc
     5401 ctcgccggtg gcgccagcgt cgaggagatc gcggtaggcg ttctccaccc gctcgcggcg
     5461 gccggcgtcc acgtccaacg gatacccctc catgggggag accagataag ccagctcctc
     5521 gcgttcgatg gccaggggct cggggcggct ctggacgggc tcgcgcggag aggtcgccgg
     5581 cggcgtggtg gcacacgccg ataggagggc cgcccatagc agcggcaccg cggcgaggcg
     5641 gagcacgaac cggactccgg cgctacgcgt ccggatggcg gcctcaggcc gcgggagggg
     5701 ccgctgcgat cgtagcgatg gcatgcttgt agatgaggat ttccttgccg tcgctctcga
     5761 gcaccacggc gaagcgatcg aaccgcttga ggcgccccag caggtgccgg ccggtcacca
     5821 gctccaggaa catctgctgt ccctctttga ggctctggaa caggtacccg tcctgaatgt
     5881 tgatattgtg cttagccata cgccctcccg agaccgcttt gcttgacgtg ctcaaagaca
     5941 tcagaaaaat aatcctccag gtcgttctgg gccggaaacc aagcgatatc gggctctttc
     6001 cggaaccagg tctcctgtcg tttggcgaag cggcgcgttt cccgcaccgt ttcaccgacg
     6061 gcttgctcca gagaccactc cccctgcacg tgccggacga tctggcgata gccgatggcc
     6121 tggaacgccg gaacgccagg ggatactccc tgcgcgagca atccggcaac ttcttccacc
     6181 catccgcctt gcaccatccg ggccacgcgg cgcgcgattc gatcgtatag gatggcgcgc
     6241 gggagcgtca atccgacgcg ccgggccgca atgcgttgag ttccaaaggg ttgctgcgta
     6301 atccaggtgg aaaggggctt cccggagacc tgcgccacct cgagcgcgcg tagcacccgc
     6361 tgggtatcgc cgggtgcgag gcgtgccgcg gtggccgggt cgagacggcg cagctcggcc
     6421 accagggcgg gcagcccctc ctcctcgagc cgccggcgta gcgcctcgcg cgtctccggg
     6481 tcccccgacg gcaccgggct gatcccctcg aacagcgccc gcaggtagag gccgctcccc
     6541 cctaccacga tcggcagccg gccgcgcccg cggatgtcgg cgatcgcctc cgcggcccgg
     6601 cgcgcgaact cccccgccga gtagcgctcg tccggctcga ggatgtcgac caggtgatgc
     6661 ggcacccgcc ggcgctcgtc cgggctcggc ttggcggtgc cgatgtcgag gccgcggtag
     6721 gcctggagcg cgtcggcgtt aacgatctcg ccgccgagcc gctcggccag ggccagggcc
     6781 agggcgcttt tgccggtggc ggtggcgccg gtgatggcga cggtggggac gggagacgtc
     6841 atctgaagag gatggcacaa aggcgcttct gggtgatccg acggatcggt cggaccagtc
     6901 ggatcaccga gcaggagagg gggccaggag aggaagcgcc tacggcctca gaagatcgtc
     6961 cggctcgccg ccgacggccg caccgcgcgc gatttgcgca ggtcccgcac gatgttgacg
     7021 cgcttgagcc agcggtcctc gccgtcgtag cgcgccctga aagcgcggcg gccgtgcacc
     7081 gccttgaagt tgtcgatgaa gcagatctct cccggctcga gcaccacgtc cttcagg
//
LOCUS       DASPJI010000836         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_732072, whole genome shotgun sequence.
ACCESSION   DASPJI010000836 DASPJI010000000
VERSION     DASPJI010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732072"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VEL74_23525"
     CDS             complement(<1..984)
                     /locus_tag="VEL74_23525"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HYG65573.1"
                     /translation="MTQSPRRTSVGGVHWPELDGLRALAILLVVARHSLRPFISEDTY
                     APVAAWGGVDLTPFLMNGWVGVDLFFVLSGFLIGRQAFRRDGWGRFWFKRVTRIVPAY
                     WACLALVAVGLTASGSWAESGRDFAAHLVMLQDYTGSVFVPAFWSLGAEEKFYLLAPL
                     LAVGLARLRSPVLQGAALAGLWLAPVVFRILATRSVVPPVPYETYFPSFRSPFHLTCE
                     TLVLGFVIAWATLHLRPEVLGPRAREAVFWSGAAGLLVALVPSALLGHIDRVTIMAMP
                     AAIGLGFGAMVLACVSGPGSYSRCLSWRGWRPLALTSFSLYLTHMMVIPSAV"
     gene            1072..1578
                     /locus_tag="VEL74_23530"
     CDS             1072..1578
                     /locus_tag="VEL74_23530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HYG65574.1"
                     /translation="MDTALLPVLPSDPSLARDTEELYDALEDLLRVYQFRDRDRICCF
                     DVSVSQCYALEGLVRRGGMTLNDLAAYLYLDKSTASRVVDALERKGYVTRLPHPKDRR
                     AVLLEATPAGRELEGKIRESILAEERQLLADFAPEVRQAMTVLIRRLARAASASVETG
                     GGSCCRIP"
     gene            1628..>2286
                     /gene="arsM"
                     /locus_tag="VEL74_23535"
     CDS             1628..>2286
                     /gene="arsM"
                     /locus_tag="VEL74_23535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HYG65575.1"
                     /translation="MNIDPQTLDPNALREVVQAKYGEAARTARTGKASSCGCGCSSSK
                     DPITSDLYEAQETAGLPAEAVLASLGCGNPTALAELKEGETVLDLGSGGGIDVLLSAR
                     RVGPTGRAYGLDMTDDMLALARENQRKAGVENVEFLKGEIEAIPLPEAAVDVIISNCV
                     INLSPEKDRVFGEAHRVLRPGGRLAISDVVATAELPAAIRGDLALYSGCVAGASSIDA
                     I"
BASE COUNT          406 a          824 c          769 g          287 t
ORIGIN      
        1 cacggcggag gggatcacca tcatgtgagt caggtacagc gagaacgagg tcagcgccag
       61 cggccgccag ccgcgccaac tcagacaccg gctgtagctg ccgggtccgc tgacgcacgc
      121 cagcaccatg gcgccgaagc ccagcccgat ggccgcgggc atcgccatga tcgtgacgcg
      181 gtcgatgtgc ccgagcagcg ccgacgggac cagcgccacg agcagcccgg cggccccgct
      241 ccagaacacg gcctcccgcg cccgcggccc caacacctcc ggccgcaggt gcagggtggc
      301 ccaggcgatg acgaagccga gcaccagcgt ctcgcaggtc aggtggaagg ggctgcggaa
      361 cgatggaaag taggtctcgt agggaaccgg cggcaccacg ctccgggtcg ccaggatccg
      421 gaagacgacc ggcgccagcc acagcccggc cagcgccgcg ccctggagca ccggggaacg
      481 caaccgcgcc agccccaccg cgagcagcgg cgccagcaga tagaacttct cttcggcacc
      541 cagcgaccag aacgccggca cgaagacgct gcccgtgtag tcctgcagca tcacgaggtg
      601 cgcggcgaag tcccggccgg actccgccca ggagcccgaa gccgtcagcc ccacggccac
      661 gagggccagg caggcccagt aggcagggac gatccgggtc acgcgcttga accagaaccg
      721 tccccagccg tcgcgcctga aggcctgccg gccgatcagg aacccgctga gcacgaagaa
      781 caggtccacg ccgacccagc cgttcatcag gaacggggtc aagtcgacgc cgccccacgc
      841 cgcgaccggc gcgtaggtgt cctcggagat gaaggggcgc aggctgtgcc gcgccaccac
      901 aagcaggatg gcgagagccc ggaggccgtc cagctcgggc cagtggacgc ccccgaccga
      961 cgtacgtctc ggcgactggg tcactgcggg tatcttagca gcggagctgg aaagccgggc
     1021 ttgcatccca gatagttgca accgccaact atcccgtgtt atcgtctctc catggacacg
     1081 gcgctcctcc ccgtcctccc ctccgatccg tccctggcgc gggacacgga ggagctgtac
     1141 gacgccctgg aagacctgtt gcgggtctac cagttccggg accgggaccg gatctgctgc
     1201 ttcgacgtct ccgtctcaca gtgctatgcc ctcgaggggc tggtgcggcg gggaggcatg
     1261 acgttgaacg acctggccgc ctatctctat ctcgacaaga gcacggcgag ccgcgtggtg
     1321 gacgccctgg agcgcaaggg gtacgtcacc cgccttcccc atccgaagga ccgccgggcc
     1381 gtcctcctcg aagccacccc cgccggccgg gagctcgaag ggaagatccg cgagtcgatc
     1441 ctggccgagg agcgccagct cctggccgac ttcgcacccg aggtccggca ggccatgacc
     1501 gttctcatcc gccggctcgc ccgcgccgcc tcggcgagcg tcgagacggg cggcggaagc
     1561 tgctgccgta tcccgtgagt ttttttggac agatagttgc aactgacaac cagaaaggag
     1621 cagtcccatg aacatcgacc cgcagactct cgatcccaac gccctccgcg aggtggtcca
     1681 ggccaagtac ggagaggcgg cccgcaccgc ccgcacgggg aaggcctcct cctgtggatg
     1741 cggctgcagc agcagcaagg acccgatcac ctcggacctc tacgaggccc aggagaccgc
     1801 cgggctgccc gccgaggcgg tgctcgcctc gctggggtgc ggcaacccca cggcgctggc
     1861 ggagctgaag gaaggggaga ccgtgctcga cctcggctcc gggggcggca tcgacgtgct
     1921 cctctcggcc cgccgggtcg gccccacggg cagggcctac ggcctggaca tgaccgacga
     1981 catgctcgcg ctcgctcggg agaaccagcg caaggcgggg gtggagaacg tcgagttcct
     2041 caagggggag atcgaggcga tcccgctgcc ggaggccgcc gtcgacgtca tcatctcgaa
     2101 ctgcgtcatc aacctctcgc cggaaaagga tcgcgtcttc ggcgaagccc atcgcgtgct
     2161 ccgtccgggt ggccggctcg cgatcagcga cgtcgtcgcg acggccgagc ttcccgcggc
     2221 gatccgcggc gacctcgcgc tctacagcgg ctgcgtcgcc ggcgccagca gcatcgacgc
     2281 gatcga
//
LOCUS       DASPJI010000837         6367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_732099, whole genome shotgun sequence.
ACCESSION   DASPJI010000837 DASPJI010000000
VERSION     DASPJI010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732099"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VEL74_23540"
     CDS             <1..549
                     /locus_tag="VEL74_23540"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HYG65576.1"
                     /translation="GLLYTTLRWCRAAGVPRPPRLAERLTELADCALARGRGLLWPWR
                     DGPEGGAEVASMPGWCNGSAGFVYLWTLAGRELGGPDWRPLAEGAAWHTWEAPDRSGN
                     LCCGLAGRAYALLHLWRHGGGAEWLERARELAESAAREIDATTDAPDSLFRGLPGVAA
                     LAADLTRPETAAFPLFEEEGWE"
     gene            814..1836
                     /locus_tag="VEL74_23545"
     CDS             814..1836
                     /locus_tag="VEL74_23545"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65577.1"
                     /translation="MLVQSGDDPEALVAELLREPEPRRRERIATDPRFRLLKVCEILA
                     TRSRELWFEDPARSMESARLAVTVAEFLDEAGYGRGLVADARALACAHLGNAHRITSD
                     LWRAEEALREAEAHHVVAGVDPLVRAEILSFQASLRDMQGRFHESAQLLDRTIAIYRA
                     AGERHREGRALIQKATVLASEGKVKDALIITRRGLPKLSFWREPQALLAARHNLTTLL
                     AADGSYLEAFETLRKTRRLTLELGEPQLYLVKLRWLEANLASQLGRLDQALAGLVHVR
                     QAFIERGLSLDVAVVSLDLAVVQARLGRKAEARRLVLEAIPLFESRGMRQDVLAALLL
                     FKDVAF"
     gene            1843..3333
                     /locus_tag="VEL74_23550"
     CDS             1843..3333
                     /locus_tag="VEL74_23550"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HYG65578.1"
                     /translation="MPAAFPLTRWRLAGLAAGLIAYVLLATLDTPLRNHPELGARPAY
                     AAAGAALMAIWWLTEAIPIYLTACVPLVLFPLTGVFGPGLGENVRQSVLPYVDPYIFL
                     FAGGMAIAAAMQQWDLHRRIALGIMQRIGSDPRRLLFGVLAATAFISLWISNTATAAM
                     LMPIGIALIAQIEAQFGVPRLRHYGMAVMLAIAYGSNVGGLGTKIGTAPNAQFAGFME
                     RLDISISFLQFIAVGLPFVVMFLPLVWWMLWRIGRQEGLAGEVGAEVIAGELARLGPV
                     KRAERIVLGVFLATAALWIAGKWLTAALKPRVTAFELTSAHVEGGIAMLAAFVLLLWR
                     TGRRPRQPILGFAALRRVPWETLLLLGGGFAMASAIQKSGLSGWMGEQMLAVRGLAPF
                     LQVLLASLAVVALSAVASNTATIAVMLVVLKDAVAPEVMTTTLFAATLAASCDFALPA
                     GTPPNAIVFGSGYVTIPRMARTGVVLDLAAAVVVAIWCWLVVRWVL"
     gene            complement(3593..4021)
                     /locus_tag="VEL74_23555"
     CDS             complement(3593..4021)
                     /locus_tag="VEL74_23555"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HYG65579.1"
                     /translation="MSETGWRHVEPIAVRWGDMDTMGHVNNAKYFTYCESARMSYFAA
                     VRMPEHREIPTQGPALAAANLNFRRQVRYPADLDVFTRVVEIGRSSFKMEYLLVQRDT
                     GERVADGHGVVVWVDYATGMSNPLPESLRRAIREYEGTPG"
     gene            complement(4018..4992)
                     /locus_tag="VEL74_23560"
     CDS             complement(4018..4992)
                     /locus_tag="VEL74_23560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ran-binding zinc finger domain-containing
                     protein"
                     /protein_id="HYG65580.1"
                     /translation="MAVREGKWRCPYCSTVNRGRDLVCSGCGSTRDEDVKFFLEEEAP
                     EVTDAALLAQARAGADWLCQFCETSNPPSATDCGNCGAEKGTSPSRPVLEIPAVPASP
                     LPAAPPASPTSRKGGCGRWLLLGGLLLLLLLAGVCFLETRRTEEPVTVTGFGWERTVE
                     VQAWRTVRDSAWEGEVPAGARVVSRSREVHHTEREQVGTERVKVGTRDLGNGFFEDVY
                     EDRPVYEERPVHRDRVTYEVERWVPVRTERAQGSDRSPSWPALRLGAGEREAGRSERY
                     YVLLQGEDAYRMELPQSRWSSFAEGQSLTAVVQGGDEVLELHGGGEVP"
     gene            complement(5008..5550)
                     /locus_tag="VEL74_23565"
     CDS             complement(5008..5550)
                     /locus_tag="VEL74_23565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65581.1"
                     /translation="MMEALRKRDIRPLLLVFAVLGLSGLEAAPAAAAEHRLGGGVHYW
                     KTVDELADDDFDIDDEGVAWVASYQYVPVGLFKLEVDVEYFPSGFGGSPETAWSPQLY
                     LVLGGKLYGAVGAGVVFSEGYEDSPSDPFYAARLGLDLTLLPRTHLDVHANYRFNDWN
                     EFEQADTDTITLGALLRFAL"
     gene            5759..>6367
                     /locus_tag="VEL74_23570"
     CDS             5759..>6367
                     /locus_tag="VEL74_23570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65582.1"
                     /translation="MAKRDNLGGGGRTAPNSPGTDFPNPDPLTASGPGLTAAGAPATQ
                     GTDPSLPDQGWGQSGFQSQGQQGQQGVGQQAKDEAKNLARDAKHQTSKVANQAKDQVN
                     QMVSQSKDQAAERLNSFASVLRDTAGKLNEQDENGVGQYADRAAQQVERLSNYLRDRD
                     VSTFLRDTETFARRHPDVFLGGVFLAGLVLARFFKASSDRDDW"
BASE COUNT          904 a         2331 c         2188 g          944 t
ORIGIN      
        1 ggcctcctct acaccacgct gcgctggtgc cgcgccgccg gcgtgccccg gccgcccaga
       61 ctcgcggaga ggctcaccga gctggcggac tgcgcgctgg cgcggggccg cggcctgctc
      121 tggccctggc gcgacggccc ggaggggggc gccgaggtag cctccatgcc cggctggtgc
      181 aacggcagcg ccggcttcgt ctatctctgg accctggccg gccgggagct cggcggcccg
      241 gactggcgcc cccttgccga aggcgccgcc tggcacacct gggaggcccc cgaccgcagc
      301 ggcaacctct gctgcggcct tgccggccgc gcctacgcac tcctccacct gtggcggcac
      361 ggcggcggcg ccgagtggct ggagcgcgcc cgggagctgg ccgagagcgc ggcgcgcgag
      421 atcgacgcca ccaccgacgc gccggacagc ctcttccgag ggctcccagg cgtcgccgcc
      481 ctggccgccg atctcacccg ccccgagacg gccgccttcc ccctgttcga ggaagagggc
      541 tgggaatgag cctccggact gcctgatctc acctggcaca gcgcgccgcc cggtaatccg
      601 taccgtcctc ccggccccga ccttcggcca gggcgccgct tccggtatca ttggaggcct
      661 ggagacgtcg atgcaccgcc gagcccccct ttcttcccag cccgacacgg cgggccttcc
      721 ttttctgcgc gagcgcagag ccctcggaga cgagaggggc cgatgaagaa ccggcggcgc
      781 gggggatccg cccctctctc cggggcggga ctggtgcttg tccagtctgg cgatgatccg
      841 gaggccctgg tcgccgagct gctccgcgag ccggagccgc ggcggcggga gcggatcgcc
      901 accgaccccc ggttccggct cctcaaagtc tgcgagatcc tggccacccg cagccgcgag
      961 ctctggttcg aggatccggc acggtccatg gaatccgccc ggctggcggt gaccgtcgcg
     1021 gagttcctgg acgaggccgg gtacgggcgc ggcctcgtcg ccgacgcccg ggctctggcc
     1081 tgtgcccacc tgggcaacgc ccaccgcatc acctccgatc tctggcgagc cgaggaggcc
     1141 ctgcgcgagg ccgaggcgca ccacgtggtg gccggcgtgg atcccctggt gcgggcggag
     1201 atcctgagct tccaggcctc gctgcgggac atgcaggggc ggtttcacga gtcggcccag
     1261 ctcctcgacc gcaccatcgc catctaccgc gcggccgggg agcgtcaccg ggagggccgc
     1321 gcgctgatcc agaaggcgac cgtcctggcc agcgagggaa aggtgaagga cgccctgatc
     1381 atcacccgca ggggcctgcc caagctctcg ttctggcgcg aaccgcaggc gctcctcgcc
     1441 gcccgccaca acctgacgac cctgctcgcc gccgacggct cctatctgga ggcgttcgag
     1501 accctccgca agacacggcg gctgaccctg gagctgggcg agccccagct ctacctcgtc
     1561 aagctgcgct ggctcgaggc caacctcgcg agccagctcg gccggctcga tcaggcgctc
     1621 gccgggctcg tccatgtccg gcaggccttc atcgagcggg gcctcagcct ggacgtggcc
     1681 gtcgtctcgc tggacctcgc cgtggtccag gccaggctgg ggaggaaggc cgaggcgcgg
     1741 cggctcgtcc tcgaagcgat ccccctcttc gagtcgcgcg ggatgcgcca ggacgtcctc
     1801 gccgccctgc tgctcttcaa ggacgtcgcg ttctaggcga ccgtgcccgc cgcgttcccg
     1861 ttgacccgct ggaggctcgc cggcctggcc gccggcctca tcgcctacgt cctcctcgcc
     1921 accctggaca cgccgctccg gaaccacccg gagctcgggg cgcgcccggc gtacgcggcg
     1981 gcgggcgcgg cgctcatggc catctggtgg ctcaccgagg cgatcccgat ctatctgacc
     2041 gcctgcgtcc cgctcgtgct cttccccctc accggcgtct tcgggcccgg cctgggggag
     2101 aacgtacggc agagcgtcct cccctacgtc gatccctaca tcttcctgtt cgccggcggc
     2161 atggccatcg ccgccgccat gcagcagtgg gacctgcacc ggcggatcgc cctcggcatc
     2221 atgcagcgca tcggcagcga cccgcggcgc ctgctcttcg gcgtgctggc cgccacggcg
     2281 ttcatctcgc tgtggatctc caacaccgcc acggccgcca tgctgatgcc catcggcatc
     2341 gccctgatcg cccagatcga agcccagttc ggcgtgccgc gcctgcggca ctacggcatg
     2401 gcggtgatgc tggccatcgc ctacggctcg aacgtcggcg gcctcggcac caagatcggc
     2461 accgcgccca acgcccagtt cgccggcttc atggagcggc tggacatctc catctccttc
     2521 ctccagttca tcgccgtggg cctgccgttc gtggtcatgt tcctccccct ggtctggtgg
     2581 atgctctggc ggatcggccg ccaggagggg ctcgccggcg aggtgggggc ggaggtgatc
     2641 gcgggcgagc tggcgcggct ggggccggtc aaacgggccg agcggatcgt gctcggcgtc
     2701 ttcctggcga cggcggccct ctggatcgcg ggcaagtggc tgaccgccgc cctcaagccg
     2761 cgggtcaccg cgttcgagct gacgtcggcg cacgtcgagg ggggcattgc catgctcgcc
     2821 gccttcgtcc tcctcctctg gcggacgggc cggcggcccc gccagccgat cctcgggttc
     2881 gcggccctgc gccgggtgcc ctgggagacg ctgctcctcc tgggcggcgg cttcgccatg
     2941 gcctccgcca tccagaagag cggcctgtcg ggctggatgg gggagcagat gctggcggtg
     3001 cgggggctcg ctccgttcct gcaggtgctg ctggcgagcc tcgccgtggt ggcgctctcc
     3061 gccgtcgcct ccaacaccgc caccatcgcg gtgatgctgg tggtcctcaa ggacgcggtg
     3121 gcccccgagg tgatgaccac cacgctcttc gccgccaccc tcgccgcctc ctgcgacttc
     3181 gccctgcccg cgggcacgcc gcccaacgcc atcgtcttcg gcagcggcta cgtcaccatc
     3241 ccgcgcatgg cgcgcaccgg cgtcgtgctc gacctcgccg ccgcggtcgt cgtcgccatc
     3301 tggtgctggc tggtggtgcg ctgggtgctg tagcccgagc gttcttcgtc gcagagcagg
     3361 gctcccgagg gccgcgaccc ggggaaaaca tcgcagagcc gcggtgattc gtcgcggatc
     3421 ccctccgctc tgcgacgaat agaagcccat ctgcgacgaa cgaaggctca tccgcgacga
     3481 acgaaggttc atctgcgacg aatgaagacc ggtttgcgac gcgggagcgt tactctgcga
     3541 cgattcagcg ggaccttgcg acgttgcggg gcagctctgc gttgacggac ccctagcccg
     3601 gcgtgccctc gtactcccgg atggcccggc gcaggctctc gggcagcggg ttcgacatcc
     3661 ccgtggcgta gtccacccag acgacgacgc cgtgaccgtc ggccacccgc tctccggtat
     3721 cccgctggac gaggaggtac tccatcttga agctgctgcg gccgatctcc accacccggg
     3781 tgaagacgtc gaggtcggcc gggtagcgga cctggcgccg gaagttgagg ttggccgcgg
     3841 ccagggcggg cccctgggtc ggaatctccc ggtgctccgg catgcggacg gcggcaaagt
     3901 agctcatgcg cgccgactcg cagtaggtga agtacttggc gttgttgacg tgccccatgg
     3961 tgtccatgtc gccccaccgt acggcgatgg gctccacgtg gcgccagccg gtctcgctca
     4021 tgggacctcc cctcctccgt ggagctccag gacctcgtcg ccgccctgca cgacggcggt
     4081 cagcgactgc ccctcggcga aggacgacca ccggctctgc ggcagctcca tccggtaggc
     4141 gtcctccccc tgaaggagga cgtagtagcg ctcgctgcgg cccgcctccc gctcgccggc
     4201 tcccaaccgc aaggcgggcc aggaggggga gcggtcgctc ccctgggccc gctcggtgcg
     4261 gaccggcacc cagcgctcga cctcgtaggt gacgcggtcg cggtggaccg gccgctcctc
     4321 gtagaccggg cggtcctcgt agacgtcctc gaagaagccg ttgcccaggt cgcgggtccc
     4381 caccttgacc cgctcggtcc ccacctgctc gcgctcggtg tggtggacct cccgggagcg
     4441 ggagacgacg cgggcgccgg ccggcacctc cccctcccag gcgctgtcgc ggaccgtccg
     4501 ccaggcctgg acctccaccg tccgctccca gccgaagccg gtgacggtga ccggctcctc
     4561 ggtccggcgg gtctcgagga agcagacgcc ggcgaggagg agcagcaaca ggaggccgcc
     4621 gaggagcagc caccggccgc agcccccctt ccgagaggtg ggcgacgcgg gaggcgcggc
     4681 aggcaacggc gaggcgggta cggccgggat ctccagcacc gggcgagagg gcgaggtccc
     4741 cttttcggcg ccgcagttgc cgcaatcagt cgccgacgga gggttggacg tctcacagaa
     4801 ctggcagagc cagtcggcgc cggcgcgggc ctgggccaga agcgccgcgt cggtgacctc
     4861 gggcgcctcc tcctcgagga agaacttcac atcctcgtcg cgggtggagc cgcagccgga
     4921 gcagacgagg tcccggcccc ggttgaccgt ggagcagtag gggcagcgcc acttgccttc
     4981 gcggaccgcc atggacctat ggatggatca gagcgcgaac cgcaagaggg cgcccagggt
     5041 gatggtgtcg gtgtcggcct gctcgaactc gttccagtcg ttgaaacggt aattggcgtg
     5101 cacgtccaga tgagtccgcg gcaggagggt caggtcgagc ccgagccggg ctgcatagaa
     5161 gggatcggag gggctgtcct cgtagccctc ggagaagacc accccggccc cgactgcacc
     5221 gtagagcttg ccgcccagca cgaggtagag ctggggcgac caggcggtct cgggagagcc
     5281 gccgaagccg ctggggaagt actccacgtc cacctccagc ttgaacagcc ccacggggac
     5341 atactgatac gaggcgaccc aggcgacccc ctcgtcgtcg atgtcgaagt cgtcgtccgc
     5401 cagctcgtcc acggtcttcc agtaatgcac cccgccgccg agccggtgct cggccgccgc
     5461 ggcgggcgcc gcctccaatc ccgacagccc cagcactgcg aagaccagca gcaacgggcg
     5521 gatatctctc ttgcgaagcg cctccatcat cttctccatc tcctctcggt ggctggaaac
     5581 gaaccatatc ccgtcctggt ccgcgagggc cagaggcggc gtggacgtcg cggcgcggcg
     5641 acattggcac gctcattgcc agtacagact cgtggatgcg gagacccgaa agggtccggt
     5701 gctcgcttca aggtgaagtc ttgcacgccg ctggaactac ttcaacgaag gaggagctat
     5761 ggcgaaacga gacaatctcg ggggaggcgg ccgtactgcg cccaactcac caggaacgga
     5821 ttttcccaat cccgatcctt tgaccgcgag cgggccgggg ctcaccgcgg cgggcgctcc
     5881 ggccacccag ggcaccgacc cgagcctgcc tgatcagggg tgggggcaga gcggcttcca
     5941 gtcccaggga cagcaggggc agcagggtgt aggccagcag gcgaaggacg aagcgaagaa
     6001 cctggcccgc gatgccaagc accagacctc caaggtcgcc aaccaggcca aggaccaggt
     6061 caaccagatg gtgtcgcaga gcaaggatca ggccgccgag cggctgaaca gcttcgccag
     6121 cgtgctgcgt gacaccgccg gcaagctcaa cgagcaggac gagaacgggg ttggccagta
     6181 cgccgaccgc gccgcccagc aggtcgagcg gctctccaac tatctccgcg accgggacgt
     6241 gagcacgttc ctgcgcgata ccgagacctt cgcccggcgt caccccgacg tcttcctggg
     6301 cggggttttc ctggcgggcc tggtgctcgc ccgcttcttc aaggcctcca gcgaccgcga
     6361 cgactgg
//
LOCUS       DASPJI010000838         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_454742, whole genome shotgun sequence.
ACCESSION   DASPJI010000838 DASPJI010000000
VERSION     DASPJI010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454742"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1791
                     /locus_tag="VEL74_23575"
     CDS             <1..1791
                     /locus_tag="VEL74_23575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65583.1"
                     /translation="RRLYGHAAGLAAAALVAVFSPQLVLWSTLASGGYTLVVGWGSLT
                     FAHLAATRRQPTLGRTALLGFLVGFGLYIYELYLVYVAILAGFALTSSFVWRAVLAPS
                     RQARQARQERSAALPLAPGQIGRAALFLAGFLVGWAPKLWTTFFGGVATKLPAYGFAS
                     PEVMRANLELLVTRCVPALLGANPAGEPELLQWVGPMTALTTVLGVLVLLAWAAAWLQ
                     AVPGTGRDLLGALRRPPGEIGMEGLLVLLVPVTALLFVLSPNPTGVLSSRYLLPCLTS
                     LPVLAGGWLVRLWRRGKLARLAAAALGLLLVGYPGAQIVLWHVGSGYLTADLRLPRSR
                     DSLHEVFRELRRQGIRGGYGWYWIAYEANFLSGEEIVIAPSDGWDRYPAYTRFVDSLR
                     RPAYLFEGGPETERVREAQFLERVRASDQPFEVRRVGQYAIYTSPRGERLLPASLVRG
                     PNLLVQPRAEIAARGVDVLAGKVTPGRILEIPARLTNRGEDLWPAFGLDGSDHYRVAL
                     SYRWLDAAGQEVAGPPALRTPLPDDVLPGASVDLVARVQVPEAPGAYRLVLTMIQEDV
                     LWFADAPEGGAAVFPVTVTAEAAAAAGGGG"
     gene            1788..>2425
                     /locus_tag="VEL74_23580"
     CDS             1788..>2425
                     /locus_tag="VEL74_23580"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HYG65584.1"
                     /translation="MRSRLRWPRATAALVLLLVGSCLGAALSSRPAPEPPPGQRANII
                     LLSVDSLRRASFRPDRLPVMNALAAGSVVFDNAVSPSSWTLPSHASLMTGLYPDRHGA
                     VDRRLRMGTEYPTLSLLLWEAGYETVGFTDKGFLDRRFGMGRGYERYDDWTVRRDPGL
                     EAVLPRAGDATGTPGSDRFDRALAFLRQRRSPERPFFLFLHTFGVHDYFYAH"
BASE COUNT          256 a          942 c          842 g          385 t
ORIGIN      
        1 cggcgcctct acggccacgc cgcagggctc gccgccgcgg ccctggtggc ggtgttctcg
       61 ccgcaactgg tgctgtggag caccctcgcc agcggcggct acacgctggt ggtgggctgg
      121 ggctcgctga ccttcgccca cctcgccgcc acccggcggc agccgaccct cgggcggacc
      181 gcgctcctcg gcttcctggt gggcttcggc ctctacatct acgagctcta cctggtctac
      241 gtcgcgatcc tcgccggctt cgccctcacc tcgtccttcg tctggagggc cgtgctggcc
      301 ccgtcgcggc aagcgcggca ggcgcggcag gagcgctccg cggccctgcc gctggcgccg
      361 gggcagatcg ggagggcggc cctgttcctg gcgggcttcc tcgtgggctg ggcgcccaag
      421 ctgtggacca cgttcttcgg cggcgtggcc accaagctcc ccgcctacgg cttcgccagc
      481 ccggaggtca tgcgggcgaa cctggagctc ctggtgaccc gctgcgtgcc cgctctcctc
      541 ggcgccaatc cggccggcga gcccgagctg ctgcagtggg tgggccccat gaccgccctg
      601 acgaccgttc tcggcgtgct cgtgctgctg gcctgggcgg cggcctggct ccaggcggtc
      661 cccgggacgg ggcgggacct cctcggcgcc ctgcggcggc cgccggggga gatcgggatg
      721 gagggcctgc tggtcctgct ggtgccggtc accgccctcc tcttcgtgct cagccccaac
      781 cccacgggcg tcctctccag ccgctacctc ctcccctgcc tgaccagcct ccccgtgctg
      841 gccggcggct ggctggtccg gctctggcgc cgcgggaagc tcgcccggct cgccgccgcg
      901 gccctcgggc tgctcctggt cgggtatcct ggagcccaga tcgtgctctg gcacgtgggc
      961 tcgggctacc tgaccgccga cctgcgcctg ccgcggagcc gggactccct gcacgaggtc
     1021 ttccgggagc tgcgccgcca gggcatccgt ggcggctacg gctggtactg gatcgcctat
     1081 gaggccaact tcctgagcgg cgaagagatc gtgatcgccc cctccgatgg ctgggaccgt
     1141 taccccgcct acacccgctt cgtggactcc ctccgccgcc cggcctatct cttcgaggga
     1201 gggccggaga cggagagggt acgggaggct cagttcctgg agcgggtccg cgccagcgac
     1261 cagccgttcg aggtgcggcg ggtcggccaa tatgccatct acaccagccc ccgcggcgag
     1321 cgcctgctgc cggcgtcgct ggtccgcggc cccaacctcc tggtacagcc ccgggcggag
     1381 atcgcggccc gcggcgtgga tgtcctcgcc ggaaaggtga cgcccggacg gatcctggag
     1441 atcccggcac ggctgaccaa ccgtggcgag gacctctggc ccgccttcgg gctcgacggc
     1501 agcgaccact accgggtcgc gctctcctat cgctggctcg acgccgccgg ccaggaagtg
     1561 gccggccccc cggccctgcg cacccccctg cccgacgacg tcctgcccgg tgcctccgtg
     1621 gatctggtgg cgcgcgtcca ggtccccgag gctccgggcg cctaccggct cgtgctcacg
     1681 atgatccagg aggacgtcct ctggttcgcc gacgcgccgg agggcggcgc cgccgtcttc
     1741 ccggtgacgg tcaccgcgga agccgcggcc gcggccgggg gtggcgggtg aggtcccgtc
     1801 ttcgctggcc tcgggccacc gcggcgctcg tcctcctcct agtcgggagc tgcctgggcg
     1861 ctgccctgtc gtcgcggccg gcaccggagc caccgcccgg gcagcgggcc aacatcatcc
     1921 tcctgtcggt ggacagcctg cgcagggcga gcttccggcc ggaccgcctc cctgtcatga
     1981 acgccctggc cgccggctcg gtggtcttcg acaacgccgt gagcccctcg tcctggacct
     2041 tgccgtccca cgcctcgctg atgaccgggc tgtatccgga ccgccacggc gccgtcgacc
     2101 ggcggctgcg gatggggacg gagtacccga ccctctcgct gctcctctgg gaggcgggct
     2161 acgagaccgt ggggttcacc gacaagggat tcctcgaccg ccgtttcggc atgggccggg
     2221 ggtacgagcg ctacgacgac tggaccgtcc ggcgcgaccc cgggctggag gccgtcctgc
     2281 cgcgcgccgg cgacgccacc ggcacaccgg ggagcgaccg cttcgaccgc gccctcgcct
     2341 tcctccggca gcgccggagc ccggagcgtc ccttcttcct gttcctccat accttcgggg
     2401 tccatgacta cttttatgcc catca
//
LOCUS       DASPJI010000839         3967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_454811, whole genome shotgun sequence.
ACCESSION   DASPJI010000839 DASPJI010000000
VERSION     DASPJI010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454811"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="VEL74_23585"
     CDS             <1..1545
                     /locus_tag="VEL74_23585"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HYG65585.1"
                     /translation="TLALAPSNQDVIYALVASNVAGSSTEPQEHYRGGMHAVFRSTSG
                     GGPGTWTAQVRNSDPTKLSRLLLSSARSAFGRECGFTSSSSNLFYGQGWYDNAIAVDP
                     TDPERVWAGGIDLYRSEDGGRSWGLASYWWAMDIFNAAAPSYSHADQHSIVFHPGYDG
                     VSNKTLFVTSDGGVSRTDDALAATARGTTAACDPRNSQLVWTALNNGYGVTQFYHGTP
                     YPDGSRYLGGTQDNGTIRGQDGAGADGWETLLGGDGAYAAIHPDNPDVVYASYQGLSL
                     QKTVDGGRTWSNSFVGINGGTLFITPYLMDPNDPERLWLGGTAIWRTDNGAGYWRAAS
                     RPLAPLGMVSSLAVARGNSERVLVGTTSGYILRTDSGLSDDQTKEWPSVRPRLGWVSS
                     VAVDPTNENVMYATYSTFGGTHVWKSTNGGATWRGSDGTGNRKIPDIPVHSIVVDPNR
                     PSRLFVGTDLGVFVSTDGGGTWAVENSGFANVITEWLTVLEGPGGPTLFAFTHGRGAW
                     RVPIRN"
     gene            1721..>3967
                     /locus_tag="VEL74_23590"
     CDS             1721..>3967
                     /locus_tag="VEL74_23590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65586.1"
                     /translation="MTLSSRTSIRLLAGTHLFILMLLAALVAPVWAAEANGPRTLSPE
                     GLRRLEAKEQKALRAPDSPDEAQEHFVAKRAPVGAKSVPVERYFTAQEEMRRMPQHST
                     ARGTFLPSRAELAEKGISYVAAAASLGAWSALGPGNIGGRTRTLVIDPRQPEVMYAGG
                     VAGGVWKTTDGGQSWRPLADLMANLAIASLAMDPANSNVLYAGTGEVFTSVGVRGSGI
                     FRTTDGGNRWERLAATSNANFHYVSDIVISSSSSSRLYAATDTGVWRSTNGGASWQRT
                     LSSDAQGGCLDLAIRKDRAEDVVFAACGKREVGGAVYRNVDAQNGDAWEQVLQEPGMG
                     RASLALAPSNQDVIYALTSSTVAGPSSEQGENYRQGMHAVFRSTSGGGPGTWTAQVRN
                     NDGNKLNRMLLSRARSAFARECGDNRPNSFSGQGWYDNAIAVDPVNPDRVWAGGIDLY
                     RSDDGGRNWGIASYWWAIFPYSSTDAPSYVHADQHLLVFHPNYDGTSNKTLYVTNDGG
                     VFRTDDALAPTAKSVTAACNSGNSQLAWTALNNGYGVTQFYHGTPFPDGKRYLGGTQD
                     NGTVQGRDGQGANAWETVLGGDGGYSAVNPVNPDIIYASYQVLSLQKSVDGGFTWRDA
                     VEGIDSFRTLFIAPYVMDPNDPERLWFGGEKIWRTDDGAEFWNTATAPLTGRGMISAL
                     AVARRNPDRVLAGTNEGYILRTGAGRTADASTEWPAVRPRPGYVSSVTFDPTNPNVVY
                     ATYSTFGGK"
BASE COUNT          648 a         1374 c         1339 g          606 t
ORIGIN      
        1 accctcgccc tggcgccttc caaccaggac gtgatctatg ccctggtggc gagcaacgtg
       61 gcggggtcgt cgaccgagcc gcaggagcat tatcgcggtg gcatgcacgc ggtcttccgt
      121 tcgaccagcg gcggcggccc cggtacctgg acggcccagg tccgcaacag cgacccgacc
      181 aagctcagcc gcctgctcct gtccagcgcg cgctccgcct tcggccggga gtgcggcttc
      241 acctcctcga gcagcaacct tttctatggc cagggctggt acgacaatgc gatcgccgtg
      301 gatcccaccg atccggagcg cgtctgggcc ggcgggatcg acctctaccg ttcggaggat
      361 ggcggccgga gctggggact ggcatcctat tggtgggcga tggacatctt caatgccgcc
      421 gcgcccagct attcccacgc tgaccagcac tccatcgtct tccatccggg ctatgacggc
      481 gtctccaaca agacgctgtt cgtgaccagt gacggcggcg tgtcccgcac cgacgacgcc
      541 ctggccgcca cggcccgggg cacgacggcg gcctgcgatc cgcgcaattc gcagctcgtc
      601 tggacggcgc tcaacaacgg ctatggcgtg acccagttct atcacggcac cccctatccg
      661 gacggcagcc gctacctggg cggcacgcag gacaacggca cgatccgggg gcaggacggc
      721 gccggtgccg acggttggga gaccctcctc ggcggcgacg gcgcctacgc ggcgatccac
      781 cccgacaatc cggacgtcgt ctatgcctcg tatcaggggc tcagcctgca gaaaacggtg
      841 gacggcgggc gcacctggag caactcgttc gtcggtatca atggcggcac ccttttcatc
      901 accccctatc tgatggaccc caacgacccg gagcgcctgt ggctcggcgg cacggcgatc
      961 tggcggacgg acaacggcgc gggctactgg cgcgcggcga gcaggcccct ggcgccgctg
     1021 gggatggtga gctccctggc cgtggcccga ggcaactcgg agcgggtgct ggtgggcacg
     1081 accagcggtt acatcctgcg gaccgactcg gggctgtcgg atgaccagac caaggagtgg
     1141 ccgtcggtcc ggccgcgcct cggctgggtc tcctccgtgg ctgtcgatcc gaccaacgag
     1201 aacgtgatgt atgccaccta ttccacgttc ggcggcacgc acgtctggaa gagcacgaac
     1261 ggcggcgcca cctggcgggg gagcgacggc acgggcaacc ggaagatccc ggacatcccg
     1321 gtgcacagca tcgtcgtgga ccccaaccgg ccgtcgcgac tcttcgtcgg caccgatctg
     1381 ggggttttcg tctccaccga tggcggaggc acctgggcgg tggagaacag cggtttcgcg
     1441 aatgtgatca cggagtggct gacggtgctc gaagggcccg gcggtcccac cctcttcgcg
     1501 ttcactcacg gccgcggcgc ctggcgcgtg ccgatccgga actgagagga aggagcgtcc
     1561 ggcaggggag tgaggggatc gcctttctcc cctcgttcct ctatcatgtt ggcatcgctg
     1621 ttctattgat ttgtggcccg agcggcgggg atgtgcccgc ttcgtggctt tccggagagg
     1681 acagatggta gtggctccac ttcccgaaag gaggctttgg atgaccctgt cctcgcgcac
     1741 ctcgatccgg ctgctcgccg gaacccacct cttcatcctg atgctgcttg cggctctcgt
     1801 cgctcccgtc tgggcggcgg aggccaacgg tcccagaacc ctgagtccgg aagggctccg
     1861 caggctcgag gcgaaggagc agaaggcact ccgtgccccc gactcgcccg atgaggccca
     1921 ggagcacttt gtcgccaagc gcgccccggt gggcgcgaag tccgtgccgg tggagaggta
     1981 cttcacggcc caggaggaga tgcggcggat gccgcagcac tcgaccgccc gcggcacctt
     2041 cctgccgtcg cgcgccgagc tggccgagaa ggggatctcc tacgtcgccg cggcggcgag
     2101 cctgggcgcc tggtccgcgc tcgggccggg caacatcggc ggccgcaccc gtaccctggt
     2161 gatcgatccc cggcagccgg aggtgatgta cgccggcggc gtggccggag gggtatggaa
     2221 gaccaccgac ggcggccaga gctggaggcc tctggccgac ctcatggcga acctggcgat
     2281 cgcctcgctg gcgatggacc cggccaactc caacgtcctc tatgccggca ccggcgaggt
     2341 cttcaccagc gtcggagtgc gcggcagcgg catcttcagg accaccgacg gcggcaatcg
     2401 gtgggagcgg ctggcggcga cgagcaacgc gaacttccac tacgtcagcg acatcgtgat
     2461 cagctccagc agcagctccc ggctctacgc ggcgacggac accggcgtct ggcgctcgac
     2521 caacggcggg gcctcgtggc agcggaccct ctcctccgac gctcagggcg gctgcctcga
     2581 cctggcgatc cgcaaggacc gcgcggagga cgtcgtcttc gccgcctgcg gcaagcgcga
     2641 ggtgggagga gcggtctacc ggaacgtcga cgcgcagaac ggcgacgcgt gggagcaggt
     2701 gctccaggag ccggggatgg gccgcgcctc cctggccctc gcgccgtcga accaggacgt
     2761 gatctatgcc ctgacgtcga gcaccgtggc ggggccgtcc agcgagcagg gggagaacta
     2821 ccggcagggc atgcacgcgg tcttccgttc gaccagcggc ggcggcccgg gcacctggac
     2881 cgcccaggtc cgcaacaacg acggcaacaa gctgaaccgc atgctcctgt cgcgcgcgcg
     2941 gtcggccttc gcccgggagt gcggcgacaa cagacccaac tccttctccg gccagggctg
     3001 gtacgacaac gccatcgccg tggacccggt caatccggac cgcgtctggg ccggcggcat
     3061 cgatctctac cgttcggacg acggcggccg caactggggg atcgcatcct actggtgggc
     3121 catcttcccc tacagcagca ccgacgcgcc gagctacgtc cacgccgacc agcacctcct
     3181 cgtcttccac ccgaactatg acggaacgtc caacaaaacc ctttatgtga ccaacgacgg
     3241 cggcgtcttc cgcaccgacg acgccttggc ccccaccgcc aaaagcgtga cggccgcctg
     3301 caattcgggt aactcacaac tcgcctggac ggcgctgaac aacggctatg gcgtcaccca
     3361 gttctatcac ggtaccccct ttcccgacgg caaacgctat ctcgggggca cccaggacaa
     3421 cggcacggtc caggggcggg acggccaggg cgccaacgcc tgggagaccg tgctgggcgg
     3481 cgatggcggc tactcggccg tgaatcccgt aaacccggac atcatctatg cctcgtacca
     3541 ggtgctgagc ctgcagaagt ccgtggatgg cggcttcacc tggcgcgatg cggtcgaggg
     3601 catcgacagc tttcgcacgc tgttcatcgc accctatgtc atggacccca acgatccgga
     3661 gcgtctctgg tttggcggcg agaaaatctg gcggacggac gacggcgcgg aattctggaa
     3721 cacggcgacg gcgcccctca ccggccgcgg gatgatcagc gcgctggcgg tggcacgccg
     3781 caatccggac cgcgtgctgg cggggacgaa cgaaggctac attctgcgga ccggcgccgg
     3841 gcggaccgct gacgcgtcga ccgagtggcc ggcggtccgg ccgcggccgg gctatgtctc
     3901 ttcggtgacg ttcgatccca ccaacccgaa cgtggtctat gccacctact cgacgttcgg
     3961 cggcaag
//
LOCUS       DASPJI010000840        11014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_164026, whole genome shotgun sequence.
ACCESSION   DASPJI010000840 DASPJI010000000
VERSION     DASPJI010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11014)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11014
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164026"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3630
                     /locus_tag="VEL74_23595"
     CDS             <1..3630
                     /locus_tag="VEL74_23595"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65587.1"
                     /translation="ERAGDLARHLVAAGVGPEEVVGLCLDRSPEMVAAILGVLAAGAA
                     YLPLESVHPPERLAWMAADAGARWIVTREPRARLARAIAEARREGAARLLDLDEVSGA
                     GEGPRGEVPLEALAYVLYTSGSTGTPKGVMLPHRALSNYLSWAGKAYGMSDGLGAPVH
                     SPLGFDLTVTSLFLPLLAGGTVTLLPEEQGVEALRDVLREDHRFGLLKLTPAHLAFLG
                     ETLGEADLTGRAGALVVGGEALLAEVVTAWRARETGPGPRARVINEYGPTEAAVGCSV
                     HELPPEAAVHGALPIGRPIANARIHLLGPDLRPVPLGAPGEIAIGGGGLARGYRGRPD
                     LTALRFVPDPVSDRPGERLYRSGDLARRLGDGDLVFLGRRDRQVKIRGFRIELAEVEA
                     ALTLHPEVTEAVAVAFPGDGGPRLAAYVVPREGGGVPAGLPDFLARRLPGHMIPTALM
                     ALPALPLTANGKVDLEALPRPETAGLPDGGYVAPRTSAEERLAVLWEEVLSTAGLRRG
                     PVGVLDSFFGLGGHSLLAARLIGRVREALGIELPLASLFQEPTVAGMARRLEEAGAAA
                     VVPEETIPRRSRQARDRPLPLSFAQERLWFLERLDPGTPLYHVAVALRAQGDLRPEAL
                     AAALTGVVRRHEALRTAFPAAQGVPVQAVAAPEESRVAVPKVDLSALPAARREGEAAA
                     RLRELARRPFDLARGPLLAARLFHLGAGESVVLVVLHHLVSDGWSMGVLIREVKALYG
                     AAVPELPPLSIQYADYAAWQRQRLQGERLAAELSWWRERLAGAPPGLDLPLDRPRPAV
                     PSRAGATRPFRLPEEAVQALALRASATPFMTVLAAFQTLLSRLAGQRDLTVGVPVAGR
                     LRPETEGLIGLFVNTLALRAELPPGLTFLEALARARAAVLEAHAHQEIPFERLVEELQ
                     PERDLSRTPLFQVMLAPRGTPAAGLDLPGVHLRPAAVDTGTAKFDLTLFLSEGDGLGE
                     AFEHSTELFDGTTIERWAGHLITLLTTAAAEPERQVADLPLLSPAERHQLLVERGEAE
                     GVVLDERGYPVPPGVVGELWLAGEPRRRTGHLARTRPDGRLEHLGSSESREEARRQEA
                     PAREPAEPRTPTEREVAAIWREVLGIEQALLGRSFFDLGGHSLLAARALARIEEQFGV
                     ELPLRQLYGAPTIETLAEAIDEALLARADDARLDELLDALEGVDDPALLEQLLASEEG
                     TGAGD"
     gene            complement(4027..4575)
                     /locus_tag="VEL74_23600"
     CDS             complement(4027..4575)
                     /locus_tag="VEL74_23600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65588.1"
                     /translation="MLRKGMLIAVALSVCAGVSAQAQPAEDFQWWRSCEELKAACKEA
                     SAEDLWWAASIDEHPGPPYPFGDGPILGTCYTHDKRSCPNCLCSDYVKLSYLGGQKGY
                     DGDRGCMTDRAAAAAKLKELCEAGACCCPQVEPKPCHVPTPVKARDPLTGSCCTFPNI
                     CSAPSDWEKPIAPHDARCDPSH"
     gene            4825..>11014
                     /locus_tag="VEL74_23605"
     CDS             4825..>11014
                     /locus_tag="VEL74_23605"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65589.1"
                     /translation="MTTLDRTERLRRLPPEKRGLLLEALRRGNAPDEIPRLAAGEPAP
                     ASFAQQRLWLLDRITPGNPAYNVPFAVRLSGRLDLPALAAALDEVTRRHDVLRTIFVP
                     GPDGPLQVVLPPAGLLLPRVDLQGLPAAARDAEARRLALELSRQPFDLARGPLIRAAL
                     LGLEPAEHLLVFNLHHTVTDGWSNGILLAEMGDVYRGARLSAPKVQYADFAAWQRRRL
                     DGEALDKLLSYWRRQLDGAPASLDLPTDRPRPAHRAHLGGVASLGLEAEPVAALEALG
                     KTRDASLFMVLLAGFQALLGRWAGTDDVVVGAPVAGRHRSEVEPLIGFFVNTLPLRGD
                     LAGDPAFAELVDRVRRVTLEAHEHAAVPFEKLVEELRPGREAGVSPLFQVAFAFQNTP
                     RPALGLPGLTAEAIPLYGGIAKFDLTLAVEPGDAGGLTVWLEHSRELFDSTTAARLLN
                     HFGRLLAAVTARPETRLSALPLLSAAERHQVAAEWSEGPAADSGATVHGLFEARAATS
                     PQAPALLADGLELTYDELSRRANRLAHHLRSLGIAPDVPVGVALQRSPAMVVALLAVL
                     KAGGAYVPLDPAYPDERLARMLEDADPPLVITSGSLADRLTFLGEGRRTVDLGREGEA
                     IAALEESDPGVAVSPDSLAYILFTSGSTGRPKGVAVPHRAVARLVRQAAFARLGPDEV
                     LLQLAPVSFDASTLEIWGALANGARLAVPGAGAPSLGDLESALGRFGVTTLWLTAGLF
                     HQMVDERPAALSGVRQLLAGGDVLSPAHVRRLLETVPGCTVINGYGPTENTTFTCCQP
                     FRHPGEVTAPVPIGRPIAGTRVHVLDAAFQPVPIGVAGELCAGGDGLARGYAGQPGLT
                     AERFVPDPTGTMGGRLYRTGDRVRFLADGRIEFLGRLDHQVKVRGFRIEPEEIETVLG
                     AHPGVRRAVVMALPDRHGDRRLAAYVVLRDGEVPAGVLQAWLRERLPSYMVPALWTFL
                     PELPLGATGKVDRRALPDPVQAPAAGRRTPPRTFTEEILAGLWAELLGVPEVGADEDF
                     FALGGHSLLATRVASRVQEAFGVALPLADLFDHPTVEDLAPRIEALRAGLPEDLAEPP
                     LVPLGREQPLPLSFAQERLWILDRFEPGTANYNIHGSVRLQGDLDVQALRRALDWVVA
                     RHEALRTIFEPAEDGATQRVLPAMEVPLELAEPGDVALEDWERRAAATSFDLARGPLL
                     RLVLLRIAADKHVLLLATHHIVSDGWSMGVLVREMTAAYAAYADGREPELPALPVQYA
                     DYAAWQRRRLSGERLERLVVHWRQRLAGAPPGLDLPADRPRPAIRTFRGGRLSLAVLG
                     EVADRLRALTRRQGASLFMTLLAAFEVLLARLSGEEDVVVGTPIAGRGRAEAEGLIGM
                     FLNTLVLRTGLAGDPPFTALLGRMREEALSAYAHQDLPFEKLLDELRPGRDLSRTPLF
                     QVFFNMLNFPSVPLEVPGLTLEVLAPPELPAKFDLTVYAAEERAGLRFDLVYNADLFS
                     GARMAEVARQYEGLLAQVAADPERPIGSLSLVTPGMAAALPDPRSPLGDEWYGPVHER
                     FVAEARRAPERLAVSDRDESWTYGELEARTRSLAAHLVAGGVGRGDVVAIYGHRSARL
                     VWAILGVMRSGAAFTVLDPAHPPSRIADCLRLAEPRGWIELAAAGPVPATAEGAVTAI
                     PCRVTLPLPGEEPLTSGEGAEIDPPLTAGDLAYLAFTSGSTGVPKGILGRHGPLSHFI
                     PWQRQEFDLRASDRYSMLSGLAHDPLQRDLFTPLQTGAAVCVPDPAEIQVPGRLADWM
                     ARERITVAHLTPAMGQLLAELPPGAPAPRLTSLRWAFFVGDILTRRDVARLRELAPEV
                     TCVNFYGSTETQRAVGYRRVEEGGARPAAGAGKENLPLGRGMKDVQLLVLGAAGGLAG
                     LGELGEIHVRSPHLAGGYMGDPAGTAARFVPDAGSPEPGRRVYRTGDLGRYLPTGEVE
                     FAGRTDFQVKIRGFRIEPGEIEAILTRHPAIRECVVLTRDGAGGEKRLVAYLVPHQEP
                     DLRSPAAGDLRGWLKDHLPEAMIPAAFVILDRLPLNPNGKVDRRALP"
BASE COUNT         1298 a         4022 c         4097 g         1597 t
ORIGIN      
        1 gagcgggcgg gcgatctggc gcggcacctg gtggcggccg gcgtggggcc ggaggaggtg
       61 gtggggctct gcctcgaccg ctcgcccgag atggtggcgg cgatcctcgg cgtgctggcg
      121 gccggggcgg cctacctgcc gctggagtcg gtccatccgc cggagcgcct cgcctggatg
      181 gcggccgacg cgggcgcgcg ctggatcgtg acgcgggagc ctcgcgcccg gctggcccgg
      241 gcgatcgccg aggctcgacg ggaaggagcc gcccgtctgc tcgacctcga cgaggtctcc
      301 ggcgccggcg agggcccccg gggggaggtg cctctcgagg ccctggccta cgtgctctac
      361 acctccggct ccaccgggac ccccaaaggg gtgatgctgc cgcatcgggc gctctccaat
      421 tatctgtcct gggcggggaa ggcgtacggg atgtcggacg ggctcggcgc tccggtccat
      481 tcgccgctgg ggttcgatct cacggtgacg agcctcttcc tgccgctcct ggccggcgga
      541 accgtgaccc tgctgcccga ggagcagggg gtggaggcgc tgcgggatgt cttgcgggag
      601 gatcaccgct tcggcctgct caagctcacc ccggcgcacc tcgccttcct cggcgagacg
      661 ctgggggagg cggacctcac cggccgtgcc ggcgccctgg tggtgggtgg ggaggcgctg
      721 ctcgccgagg tggtgacggc ctggcgagcg cgggagaccg ggcccgggcc gcgcgcccgc
      781 gtcatcaacg agtacggccc gaccgaggcg gcggtggggt gctccgtcca cgagctgccg
      841 ccggaggccg cggtccacgg cgcgctgccg atcggcaggc cgatcgccaa tgcccgcatc
      901 cacctcctcg gcccggacct gcggccggtc ccgctggggg cgccggggga gatcgccatc
      961 ggcggcggtg gcctcgcccg ggggtaccgg ggccgcccgg acctcaccgc gctacggttc
     1021 gtgcccgatc ccgtcagcga ccggccggga gagcgtctct accgttcggg cgacctggcc
     1081 cggcggctcg gcgatggtga cctggtcttc ctgggccggc gcgaccggca ggtgaagatc
     1141 cgcggcttcc gcatcgagct ggcggaggtg gaggccgccc tgacccttca tccggaggtc
     1201 accgaggccg tggccgtggc gttccccggt gacggaggcc cccggctcgc cgcctatgta
     1261 gtgccccggg agggtggagg ggtgcccgcc ggcctgccgg acttcctggc ccgccggctc
     1321 cccggccaca tgatccccac ggccctgatg gcgctgccgg ccctgcccct caccgccaac
     1381 ggcaaggtcg acctggaggc gctcccccgt cccgagacgg ccggcctccc ggacggcggg
     1441 tacgtggcgc cccggacctc tgccgaggag aggctcgccg tcctctggga ggaggtgctg
     1501 agcacggcgg gcctccggcg gggtccggtc ggcgtcctcg acagcttctt cggactgggc
     1561 ggccactcgc tcctggcggc gcggctgatc gggcgggtgc gggaggcgct gggaatcgag
     1621 ctgccgctgg cgagcctgtt ccaggagccc accgtggccg gcatggcgcg gcggctcgag
     1681 gaagccggcg ccgcggcggt cgtcccggag gagacgatcc cgcgccgttc ccggcaggcg
     1741 cgcgaccgcc ctctccccct ctccttcgcc caggagcggc tctggttcct ggagcgtctc
     1801 gatccgggca cgcctctcta ccacgtcgcg gtggcgctcc gcgcccaggg tgacctgcgg
     1861 ccggaggcgc tggccgcggc cctgaccggc gtggtccgcc gccacgaggc gctgcgcacc
     1921 gccttcccgg cggcgcaggg ggtgccggtc caggcggtcg cagcaccgga agaatcgcgg
     1981 gtggccgtgc cgaaggtgga cctctcggcg ttgcccgcgg cgcggcggga gggggaggcg
     2041 gcggcgcgtc tccgtgagct ggcgcgccgt cccttcgatc tcgcccgcgg tcccctgctg
     2101 gcggcgcggc tcttccacct gggcgccggg gagagcgtcg tgctggtggt cctgcaccac
     2161 ctcgtcagcg acggctggtc catgggggtg ctgatccggg aggtgaaggc gctctatggg
     2221 gccgccgtcc ccgagctccc gccgctatcc attcagtacg ccgactacgc ggcctggcag
     2281 cggcagcggc tgcaggggga gcggctggcg gccgagctgt cctggtggcg cgagcggctg
     2341 gccggcgcgc cgccgggcct ggacctcccc ctggaccggc cgcgcccggc cgtgccgtcc
     2401 cgcgccgggg ccacccggcc gttccggctg ccggaggagg ccgtgcaggc gctggccctc
     2461 cgcgcctcgg ccacgccgtt catgacggtg ctggcagcct tccagacgct cctctcccgg
     2521 ctcgcgggcc agcgggacct gacggtgggg gtgccggtgg cggggcgtct ccgtcccgag
     2581 acggagggcc tcatcggcct gttcgtcaac accctggcgt tgcgcgccga gctgcctccc
     2641 gggctgacct tcctggaggc gctggccagg gcacgggcgg cggtgctgga ggcgcacgcc
     2701 caccaggaga tccccttcga gcgattggtg gaggagctgc agccggagcg ggacctctcc
     2761 cgcacgccgc tcttccaggt gatgctcgcg ccccggggca ccccggccgc gggcctggac
     2821 ctgcccggcg ttcacctccg cccggcggcg gtcgacaccg ggacggccaa gttcgacctg
     2881 accctcttcc tgtccgaggg ggacggtctg ggagaggcct tcgagcacag cacggagctc
     2941 ttcgacggta ccaccatcga gcgttgggcc gggcatctga tcaccctgct cacgacggcg
     3001 gcggccgagc cggagcggca ggtggccgac ctgcccctcc tctcgccggc ggagcgccat
     3061 cagttgttgg tggagcgggg cgaagcggaa ggcgtggtgc tggacgagag agggtatccg
     3121 gtgcctcccg gagtggtggg ggagctctgg ctggcggggg agcctcgccg ccggacgggc
     3181 cacctggcac ggacccggcc ggacgggcgg ctggagcacc tgggctccag cgagtcccgg
     3241 gaggaggccc ggaggcagga ggcgccggca cgggagccgg cggagccgcg cacgccgacg
     3301 gagagagagg tggcggccat ctggcgcgag gtcctgggga tcgagcaggc gctcctcggc
     3361 cggagcttct tcgaccttgg ggggcactcc ctcctcgccg cccgcgccct ggcccggatc
     3421 gaggagcagt tcggcgtcga gctgccgctg cgccagctct acggcgcgcc gaccatcgag
     3481 acgctggcgg aggccatcga cgaggcactc ctcgcccgtg cggacgacgc ccgcctcgac
     3541 gaattgctgg acgccctgga aggggtggac gatcccgcgc tcctcgagca gctcctggcg
     3601 tccgaggagg gcaccggagc gggggactga ggtggaaggg ctgtgtcccg gaacacaata
     3661 ccctcgtccc ggatcacgat ccggccttcc cggatcactc ttcgtgttcc ccggaacaca
     3721 atccggggcc ggcggatcac ttgcggagca ggaaggaaca ctccgcaacg ttcccggaac
     3781 actattcctg ctccggagat cattgccgga acagggatgg cgtccgggga agcggggaga
     3841 atgaccctat ccggcgccgt tgtgatccgg gaaccttccc gaatgatccg ggaaggttcc
     3901 tttgtgttcc gggactgggg ggagaccgct gtttgtgttc ccggaacact ccgggaacac
     3961 aacgcgatct cgtgggccgg cgcgctcacg ggagcgcccg gggaggcgct cccgcggccg
     4021 gtccgtctag tggctcgggt cgcagcgcgc gtcatgggga gcgatgggct tctcccagtc
     4081 gctcggcgcc gaacagatgt tggggaaggt gcagcaggag ccggtgaggg gatcccgtgc
     4141 cttcaccggc gtcgggacat ggcagggctt gggctccacc tgcgggcagc agcaggcccc
     4201 cgcctcgcag agctccttga gcttggccgc cgcggcggca cggtccgtca tacatccccg
     4261 gtcaccgtca tagcccttct gtccgccgag ataggacagc ttgacgtagt ccgagcagag
     4321 gcaattcgga cagctcctct tgtcgtgggt gtagcaggtt cccaggatgg ggccatcgcc
     4381 aaaaggatac ggcgggcccg ggtgctcgtc gatcgaggcc gcccaccaga gatcctcggc
     4441 cgacgcctcc ttgcaggccg ccttcaactc ctcgcaggag cgccaccact ggaagtcctc
     4501 ggccggctgg gcctgagccg agactccggc gcagaccgac agtgctacgg caatcagcat
     4561 tcccttgcga agcatcgagt cctcctcttt tgattaatca gagattaaca tatgttttct
     4621 gtaactctcc ccagccgcgt cccgtttcgg ccggagcgag caccggttac tagcgagagg
     4681 ccggggtcgc gcggtaccgg tctcgtcgct cctcggtggt ccaggattgc ctcggcaatg
     4741 tgcgacgccg caggccccgt ccgcgtcggg gggcttggca actgcctcgc gtgacggacg
     4801 gctggagact gcgctgaaat acggatgacg acgctcgatc gaacagaacg gctccgccgc
     4861 ctcccgcccg aaaagcgggg gctgctgctg gaggcattga ggcggggcaa cgcccccgat
     4921 gagatcccgc gtctggccgc cggggagccc gccccggcct cgttcgccca gcagcggctc
     4981 tggctgctcg accggatcac ccccggcaac ccggcctaca atgtgccgtt cgcggtgcgc
     5041 ctctccggcc gtctcgacct gccggccctc gccgccgcgc tggacgaggt gacgcggcgc
     5101 catgacgttc tgcgcacgat cttcgtcccg ggacccgacg gcccgctcca ggtggtgctc
     5161 cctccggccg gtctgctcct gccgcgcgtg gacctccagg ggctgcccgc ggccgcccgc
     5221 gacgcggagg cgcggcgcct ggcgctggag ctgtcccggc agccgttcga cctcgcccgg
     5281 ggaccgctga tccgggccgc gctcctcggt ctggagccgg cggagcacct gctggtcttc
     5341 aatctccatc acaccgtcac ggacggctgg tcgaacggca tcctgctcgc ggagatgggg
     5401 gacgtctacc gtggcgcgcg gctctccgcg ccgaaggtcc agtacgccga cttcgcggcc
     5461 tggcagcggc ggcggctcga cggggaggcg ctggacaagc tcctttccta ttggcgccgg
     5521 cagctcgacg gcgccccggc gtcgctggac ctgcccacgg accgcccgcg gccggctcac
     5581 cgcgctcacc tgggcggcgt cgcctcgctc gggctcgagg cggagcccgt ggcggcgctg
     5641 gaggctctcg gcaagacgcg ggacgccagc ctgttcatgg tccttctggc cggattccag
     5701 gcgctgctgg gccgctgggc cggaacggac gacgtggtgg tgggtgcgcc cgtggcgggg
     5761 cgccaccgtt ccgaggtcga gccgctcatc ggcttcttcg tcaacacgct gccgctgcgg
     5821 ggggacctcg ccggcgatcc ggccttcgcc gagctggtgg accgggtacg gcgggtgacg
     5881 ctggaagccc acgagcacgc cgcggtgccg ttcgagaagc tggtggagga gctgcgcccg
     5941 ggccgcgagg ccggggtcag cccgctgttc caggtggcct tcgccttcca gaacaccccc
     6001 cggccggcgc tcggcctgcc cggcctcacc gccgaggcga tcccccttta cgggggcatc
     6061 gccaagttcg acctgacgct cgccgtcgag ccgggtgacg cgggcgggct cacggtctgg
     6121 ctggagcaca gccgcgagct gttcgactcc acgaccgcgg cgcgcctcct aaatcacttc
     6181 ggccgccttc tggcggcggt cacggcaagg ccggagaccc ggctgtccgc gctgccgctg
     6241 ctctctgcgg ccgagcggca ccaggtagcg gccgagtgga gcgagggccc ggcggcggat
     6301 tccggcgcca cggtccacgg cctgttcgag gcccgggcgg cgacctcgcc ccaggctccg
     6361 gctctcctgg cagacggcct cgagctcacc tatgacgagc tgagccgccg ggccaaccgc
     6421 ctcgcccatc acctgcggag cctggggatc gcgccggacg tcccggtggg cgtcgccctg
     6481 cagcggtcgc ccgcgatggt ggtggcgctc ctcgccgtgc tcaaggccgg tggcgcctac
     6541 gtgcccctgg accccgcgta tcccgacgag cggctggccc ggatgctgga ggacgccgat
     6601 ccccccctgg tgatcacctc cgggtcgctg gcggaccgcc tcaccttttt gggagagggg
     6661 cggcggaccg tcgacctggg ccgggagggg gaggccatcg cggcgctgga ggagagcgat
     6721 ccgggggtgg cggtctcgcc cgacagcctc gcgtacatcc tcttcacctc gggctccacc
     6781 ggccggccca agggggtggc ggtgccgcac cgcgccgtgg cccgcctcgt gcggcaggcc
     6841 gccttcgccc ggctcggccc ggacgaggtg ctgctccagc tcgccccggt ctccttcgac
     6901 gcctcgacgc tggaaatctg gggggccctg gccaacggcg cccggctggc cgtcccgggc
     6961 gcgggcgcgc cgtccctggg ggatctcgag agtgccctgg gccggttcgg tgtgacgacc
     7021 ctctggctca ccgccggcct gttccaccag atggtggacg aacggccggc ggccctgtcc
     7081 ggggtgcggc agctcctcgc gggcggcgac gtgctgtcgc cggcgcacgt gcggcgcctg
     7141 ctcgagacgg tccccggctg cactgtgatc aacggctatg gccccaccga gaacaccacc
     7201 ttcacctgct gccagccgtt ccgccaccct ggcgaggtga cggcgccggt gcccatcggc
     7261 cggcccatcg ccggcacccg ggtccatgtg ctggacgcgg cgttccagcc ggtgcccatc
     7321 ggcgtggcgg gggagctgtg cgccggcggc gacgggctcg cccggggcta tgccggccag
     7381 cccggcctca cggcggagcg cttcgtcccc gatccgaccg gaaccatggg cggccgcctc
     7441 taccgcaccg gcgaccgggt gcgcttcctc gccgatggcc ggatcgagtt cctcggccgc
     7501 ctcgaccatc aggtcaaggt gcgcggcttc cggatcgagc cggaggagat cgagaccgtg
     7561 ctcggggccc accccggagt ccgccgggcg gtggtgatgg ctttgcccga ccggcatggc
     7621 gaccggcggc tggccgccta cgtggtcctc cgcgacggcg aggtcccggc gggcgtcctc
     7681 caggcctggc tgcgggagcg cctgccctcc tacatggtgc cggccctctg gaccttcctg
     7741 cccgagctgc ccctgggcgc cacgggcaag gtcgaccgcc gggcgctgcc cgacccggtg
     7801 caggctcccg ccgccggccg gcggacgccg ccgcgcacct tcaccgagga gatcctggcc
     7861 gggctctggg cggagctcct gggggttccc gaggtggggg cggacgagga tttcttcgcc
     7921 ctgggcggcc actccctgct cgcgacccgc gtcgcgtccc gggtgcagga ggccttcggc
     7981 gtggcgctgc cgctggcgga cctcttcgac cacccgacgg tggaggatct ggcgccgcgg
     8041 atcgaagcgc tgcgggccgg cctcccagag gacctggccg agcccccgct cgtgcctctg
     8101 ggtcgggagc agccgctgcc cctctccttc gcccaggagc ggctgtggat ccttgaccgc
     8161 ttcgagcccg gcacggccaa ctacaacatc cacggctcgg tccgcctgca gggagacctc
     8221 gatgtgcagg ccctgcgccg cgccctcgac tgggtggtgg cgcgccacga ggcgctgcgc
     8281 accatcttcg agccggccga ggacggggcg acgcagcgcg tgctgcccgc catggaggtg
     8341 ccgctggagc tggccgagcc gggcgacgtg gccctggagg actgggagcg gcgggcggcc
     8401 gccacttcgt tcgacctggc gcgcggcccc ctgctgcgcc tggtgctgct gcggatcgcg
     8461 gcggacaagc acgtcctcct cctcgccacc caccacatcg tctcggacgg ctggtccatg
     8521 ggcgtgctgg tgcgggagat gaccgccgcc tacgccgcat acgctgatgg cagggagccc
     8581 gagctgcccg cgctgccggt ccagtacgcc gactacgcag cctggcagcg ccggcggctc
     8641 tctggggagc gcctggagcg gctggtcgtc cactggcgcc agcgtctggc gggcgccccc
     8701 ccgggcctgg acctgccggc ggaccggccg cggccggcga tccgcacctt ccgcggcggc
     8761 cgcctgtcgc tggcggtcct aggagaggtg gcggaccggc tgcgggccct cacccggcgc
     8821 cagggggcca gcctcttcat gaccctgctc gccgccttcg aggtgctgct ggcgcgccta
     8881 tccggggagg aggacgtggt ggtgggcact cctatcgccg ggcgcggccg ggccgaggcc
     8941 gaggggctga tcggcatgtt cctcaacact ctggtgctgc gcaccggcct cgccggcgat
     9001 ccccccttca ccgccctcct cggccggatg cgggaggagg cgctgtcggc ctatgcccac
     9061 caggacctgc cgttcgagaa gctcctggac gagctgcggc cagggcgcga cctgtcgcgc
     9121 acgccgctct tccaggtctt cttcaacatg ctgaatttcc cgtcggtccc cctggaggtg
     9181 ccgggcctca cgctggaggt gctggcgccg ccggagctgc cggcgaagtt cgacctcacg
     9241 gtctatgccg ccgaggagcg ggcggggctc cgcttcgacc tcgtctacaa cgccgacctc
     9301 ttctccggcg cgcggatggc cgaggtggcg cggcagtacg aggggctcct ggcgcaggtg
     9361 gcggcggatc cggagcggcc catcggcagc ctctccctgg tcacccccgg catggcggcg
     9421 gcgctgcccg acccgcggtc ccctctggga gacgagtggt acggcccggt gcacgagcgt
     9481 ttcgtggccg aggcgcgacg cgctcccgag cggctggcgg tcagcgaccg ggacgagtcc
     9541 tggacctatg gcgagctgga ggcccgcacc cgcagcctgg cggcgcatct cgtggcgggc
     9601 ggagtggggc ggggcgacgt ggtggccatc tacggtcacc ggagcgcccg cctcgtctgg
     9661 gcgatcctgg gggtgatgcg gtccggggcg gccttcaccg tgctggaccc ggctcatcct
     9721 ccatcgcgga tcgccgactg cctccggctg gcggagccca ggggctggat cgagctggcg
     9781 gccgctgggc cggtgccagc gacggcggag ggggcggtga cggcgatccc gtgccgggtg
     9841 acgttgcccc tcccaggcga ggagcctcta acctctggag aaggggcgga gatcgatccc
     9901 cccctcacgg cgggcgacct ggcctatctc gcctttacct cgggatcgac gggggtgccc
     9961 aaggggatcc tgggccggca cgggccgctg tctcacttca tcccctggca gcggcaggaa
    10021 ttcgacctgc gggcgtcgga ccgctacagc atgctgtcag ggcttgccca cgaccccctg
    10081 cagcgggacc tcttcacgcc gctgcagacc ggcgccgcgg tctgcgtacc cgatccggcg
    10141 gagattcagg tgccgggacg cctcgccgac tggatggcgc gggagcggat caccgtggcg
    10201 cacctgacgc cggccatggg ccagctcctg gccgagctgc cgccgggggc gccggcaccg
    10261 cgcctgacct ccctgcgctg ggccttcttc gtcggcgaca tcctgacccg ccgggacgtg
    10321 gcgcgcctgc gcgagctggc gccggaggtc acctgcgtca acttctatgg ctccaccgag
    10381 acccagcgcg ccgtgggcta ccgccgggtg gaggaggggg gagcccgccc tgccgccggc
    10441 gccggcaagg agaacctccc cctgggccgc ggcatgaagg acgtccagct cctcgtgctc
    10501 ggcgccgcgg gcggtctcgc gggcctcggc gagctgggag agatccacgt acgcagcccc
    10561 cacctcgccg ggggctacat gggagatccc gccgggaccg ccgcgcgctt cgtgcccgac
    10621 gccggctctc ccgagccggg ccgccgtgtc taccgcaccg gcgacctggg gcgttacctg
    10681 cccacggggg aagtggagtt cgcgggccgc accgacttcc aggtgaagat ccgcggcttc
    10741 cgcatcgagc cgggggagat cgaggccatc ctcacgcgcc accccgccat ccgggagtgc
    10801 gtcgtcctca cgcgggacgg agcgggtgga gagaagcgcc tcgtcgccta cctggttccc
    10861 caccaggagc cggacctccg gtccccggcg gccggagacc tccgcgggtg gctcaaggac
    10921 catctccccg aggcgatgat ccccgcggcc ttcgtgattc tggaccgcct gccgctcaac
    10981 ccgaacggca aggtggaccg ccgggccctg cccg
//
LOCUS       DASPJI010000841        16130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_276294, whole genome shotgun sequence.
ACCESSION   DASPJI010000841 DASPJI010000000
VERSION     DASPJI010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16130)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16130
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276294"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3689
                     /gene="rpoB"
                     /locus_tag="VEL74_23610"
     CDS             <1..3689
                     /gene="rpoB"
                     /locus_tag="VEL74_23610"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HYG65590.1"
                     /translation="YFGDIPLMTDNGTFIINGTERVIVSQLHRSPGVFFTKEGPRAYL
                     AKIIPYRGSWVEFEYDQKDVLSVRIDRKRKFHGTVFLRALGLESDEQILRQFYTPVGL
                     SSEGGGSFTLHVTPKVLEPERARDRQTRGRREAYPVFAGIVLGKEHIGKLDTGQSLAF
                     EVGAADLERAFFIGDVVDLDTGEVIFEANELVPEDIEERMDGRNHTPVEVFFPDWELT
                     GATLSNTLAKDNTRNSKEALIEIYRRMRPGDPPTLESARSLFYGMFFDAKRYDFSRVG
                     RFKFNIKLDTEVPVDQKTLSADDFFRVIEYLLRLQKDVGRVDDIDNLGNRRVRAVGEL
                     LENQFRIGLVRMERAIKEKMSVHQDIDSAMPHDLINSKPVIAAIKEFFGSSQLSQFMD
                     QTNPLSEVTHKRRLSALGPGGLSRERAGFEVRDVHATHYGRICPIETPEGPNIGLISS
                     LATYARINDYGFIESPYKKVENGRVLDHFRIMKVGDGTFRLGQIVTGDELEAENERLE
                     KAGKRAVSVEPHAFYLSAWEEEKYIVAQANARLDEKGYFVNDRIISRAGGDFVTVERD
                     RIDYMDISPKQLVSVAAALIPFLENDDANRALMGSNMQRQAVPLLRSEAPVVGTGLEG
                     IVAQDSGAVVLTKRGGVVDSVDAERIIVRVEGEDLETGETKDFGADIYQLIKFRRSNQ
                     NTCITQKPVVYEGMRVKKGDVLADGPCTERGELALGRNVLVAFMPWRGYNFEDAILIS
                     EKLVREDYYTSIHIEEFEIEARDTKLGPEEITRDIPNVSESALKDLDESGIVRLGASV
                     KAGDILVGKVTPKGETQLTPEEKLLRAIFGEKAGDVRDASLKVPPGIEGTIVDVKIFS
                     RKGVEKDQRAKDIELEEIGRLEKNIKDEVRILNEERNKKVSDLLVGKKVSAPVKSRTG
                     QELLKAGDAITLETLERLTRQEILRLPVDERRLIDAAEIVYKKTDSHIEVLHRVNEER
                     IGLLQKGDELPPGVIKLVKVYVAMKRKLQVGDKMAGRHGNKGVISRILPEEDMPYLPD
                     GTPVEIVLNPLGVPSRMNVGQILETHLGWAGMVLGVRFATPVFDGAREPDIKSLLEEA
                     NLPLSGKTELHDGMTGEAFEQKVTVGYIYMLKLSHLVDDKIHARSIGPYSLITQQPLG
                     GKAQFGGQRFGEMEVWALEAYGAAYTLQELLTVKSDDVEGRSKVYEAIVKGDVPEPPG
                     LPESFNVLVRELQSLCLDVELLKV"
     gene            3701..7930
                     /gene="rpoC"
                     /locus_tag="VEL74_23615"
     CDS             3701..7930
                     /gene="rpoC"
                     /locus_tag="VEL74_23615"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HYG65591.1"
                     /translation="MQPFSSFDKPQSLKDFNAIKISIASPDKIRAWSYGEVTKPETIN
                     YRTFKPERDGLFCAKIFGPITDWECLCGKYKRMKHRGVICDKCGVEVTRSRVRRERMG
                     HIELACPVSHVWFFKGLPSRIGHLLDISLRDLERILYFESYVTIDPGETELKERELLT
                     EERFREMRDKYGDETFAAKMGAEAIKELLARIDVDELAEELRIIMRTETSQIRRLKAA
                     KRLKVVDAFRRSGHRPEWMILDVIPVIPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKKLLELRAPDVIVRNEKRMLQEAVDALFDNGRRGRVLKGSNNRPLKSLSDTLKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPELKLSQCGLPKKMALELFKPFIYNRLEEKGLVG
                     TIKAAKELVEAEVDEVWDALEEVIQDHPVLLNRAPTLHRLGIQAFQPTLIEGKAIKIH
                     PLVCAAFNADFDGDQMAVHVPLSPRAQIESKVLMLASNNILSPAHGRPLAVPSQDLVL
                     GGYYLTLAKPGAKGEGRVFPDFDSVLLALEGGEVETLSTIKVRYSGRCIHLETQLDKQ
                     DIVHAEVEELENKLIETTVGRVILNMHLPKEIPYINGLLKKRGLQDLVGYCFITLGNE
                     ATVRMLDEVKEVSFLYATRAGISFGVDDMVVPSRKPELLDLARKEVVEIENQRSAGVI
                     TAGERHNKIIDIWHRTTEKVSEEMFREMREVEKRSGEFNPINLMADSGARGSREQVRQ
                     LAGMRGLMSKPSGEVIETPITANFREGLSVLQYFISTHGARKGLADTALKTADSGYLT
                     RRLVDVAQDVIINEVDCGTIDGIVVSAIMEGGEILEPLRDRIVGRVSQEDIYDPMTGD
                     MLVEVNQEITETLGNSVQAAGIERVRIRSVLTCEARRGVCARCYGRNLATGRMVEIGE
                     AVGVIAAQSIGEPGTQLTLRTFHIGGTAARIAAQTQRRSKVEGIAEFERITTVQTPDG
                     TRIVKFLNVNTVESKKGALVVINRSGKLLITDEKGRERERYALTYGSQLLVHEGDEVQ
                     PGTELVTWDPFTYAILSEIGGRIEFQDIVEGENVREETDKVTGLSQKIIVEASANEKR
                     VPAIIIHGEERDKRYLLPSGSHLVVQNGQDVHPGDTLVKIPRETTKTKDITGGLPRVV
                     ELFEARSPKEPAIITEIDGTVHLGDVHKGMRKVIVESDAGETREYLVPRSIHINVQES
                     ERVRAGDPLIDGAINPHDVLQVLGEKELQRYLVDKIQEVYRSQSVAINDKHIEVIVRQ
                     MMRSVKIEEVGDTEFLIDEQVDRWKFQEENERVIAAGGTPAIGRPLLLGITKASLSTD
                     SFISAASFQETTRVLTEAAISGKVDGLRGLKENVIVGRVIPAGTGMDYYHDFHLAEDF
                     VEPEPEAEEELFFEFGEEEIRSLPQEVLEGD"
     gene            8087..8563
                     /locus_tag="VEL74_23620"
     CDS             8087..8563
                     /locus_tag="VEL74_23620"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG65592.1"
                     /translation="MAAVPDLGRYVAVREYGPADFETVYAIDCAAFEEGRRMSREKLR
                     HHLGTPNTRALVAEERGTVVAYALACVKAQGLGYLKVLAVVPGLQSRGIGSRLMADIE
                     AWLWESGAGAIMLETANDETSARDFYERHGYFVLEVLDRFYEDGTGAFRMMKDPEM"
     gene            8598..9032
                     /locus_tag="VEL74_23625"
     CDS             8598..9032
                     /locus_tag="VEL74_23625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG65593.1"
                     /translation="MEIERAAFPPSIAYSRRDLRRLFAARRCRTLVAEVDGAVAGFVT
                     GCREPRGIGRVRSLDVAPERQGEGIGSRLLAAIEEWLREQGSAALLLETAPGARGFYE
                     RHGYRIDERLIDYYREGLDAFRMVKMVKPVEDRGAPAVLPSW"
     gene            9050..9949
                     /locus_tag="VEL74_23630"
     CDS             9050..9949
                     /locus_tag="VEL74_23630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65594.1"
                     /translation="MPNRLVLALILLVLPALPVDADTVYLLNGRTFEGVIAEVTDSQV
                     RIRMQGGVLSLPKTQVLRVEESDSDYGEYLRRKGSLQRSTAAAGDWLELARWAKARGL
                     QQAAREAGLEAAAIEPGLAGLEPLLRGYGYVLDKDLDRWIPYADSMRRKGFVQANGQW
                     ITREEYASRQSRQRDEEIARLKAAREAADAARIAREAELAVAELELRTEKLRSASAAP
                     QVPYGTYGMPMYVVPGYFAPPLVVVPGGWSHGGGSHHRPRPSAPSGGGGHRPSTGGGH
                     NRSSYLHQPGSLIPGRLVPMSTN"
     gene            10079..10831
                     /locus_tag="VEL74_23635"
     CDS             10079..10831
                     /locus_tag="VEL74_23635"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYG65595.1"
                     /translation="MVEPELSLRELSLFEGVDEARLRQLESALARRSFDSNELVFSKD
                     DRCDGVYVVARGGVTIRSEVAGQPVERVRELRQGELFGEIELLEGSRRHFSARAVEPT
                     TVYQIPADPLWEFLRSHPPVEARLRSLAIHRRTSRLRLLLSPQTRRREPRIWIDRPVL
                     LTLGDGSRCEARLENLSNSGACVSGAPAAWLPGAEVSFALGAGKIPELLRVRCTVRWR
                     DGDAVGLAFDGVGQAHRRRVDQTVRHLVGSPP"
     gene            10841..11728
                     /locus_tag="VEL74_23640"
     CDS             10841..11728
                     /locus_tag="VEL74_23640"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HYG65596.1"
                     /translation="MRVVAVINRGAGTVLRQQGVDPAELRRMFAEAGVEAEIELVAGE
                     EITAAARAAVSGGAEAVLAGGGDGTIRAVAGALAGGTVPLGVLPLGTRNHFARDLGIP
                     IDLPGAVGVVASGQVRRLDLGEVNGEVFVNNSILGFYPPLVRVRDWQRRNLSRGKGLA
                     TAIALLRVLPRLPALRVRVKADGHEAVHETRFVFVGNNEYQMSLFSYGARQRFDSGSL
                     YLYVARPRGRLGLVGLALLGLVRDVIRTPHFDQWMLPEFTIETRQRALPVYLDGEVTL
                     MRPPLHYRTRERALSVILP"
     gene            complement(11743..11976)
                     /locus_tag="VEL74_23645"
     CDS             complement(11743..11976)
                     /locus_tag="VEL74_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016862679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGxxGxxG family protein"
                     /protein_id="HYG65597.1"
                     /translation="MRKTAFILSLMFLLTLGLAPAYAQTADDGTYATQTEDDGPDLGW
                     IGLLGLAGLLGLRKREPVYRDTTRTATNPTTGR"
     gene            12290..12994
                     /locus_tag="VEL74_23650"
     CDS             12290..12994
                     /locus_tag="VEL74_23650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65598.1"
                     /translation="MTTKTIRTAVVLTGLLVAVSAPSWAQRRAYERDGAFRLHAGLFT
                     PDGESEYWDDKAVDFTGDAQDLENVSIGGDYLHSLGPRLSLLFSGNYFQGDTTQSYVD
                     FVDNFGDRIRHDTTLEIASLTAGLVLHLTEPGAAIRPYIGVAGGLYSWSVVEEGDFID
                     EDNLEIFSARLESDGNVLGYYGLAGLEVPIGRTVSLFGEGRWSQVEDELSGDLEGLGD
                     IDLSGRQLAVGISWSL"
     gene            complement(13073..15649)
                     /gene="lon"
                     /locus_tag="VEL74_23655"
     CDS             complement(13073..15649)
                     /gene="lon"
                     /locus_tag="VEL74_23655"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HYG65599.1"
                     /translation="MTEILTLPVLPLREFVLFPGVTAPINAGRPGTLRAIEAALNTPD
                     RLVFAVSHREEAQVPTPEVLHTIGTVARIGQLQRGLAGMQLLLHGEHRGIAMRITEKD
                     GFLQAVVREAEEMKPLNANDPAFVALFREARERAAELGKKSGLPEEVVQQVLEGVQDP
                     SRLADLVAAYTDVTPAQRQMLLETLSVEERLRRVLVHVQRQLEVLEAQEDIKTKVQEE
                     LGDRQREMFLREQLKAIQKELGEGDNSAEIEELREKLAGLDLPPDARKEVDRELGRLS
                     RMGREAMESQVIRTYLETIAELPWSERSQEHLDLPEAAKILEEDHYALGGVKDRVLEF
                     LAVRQLREEQAARRRAEAEADAAAEAAELAEAAEASHVIGDPEDHFGEEAVQAATTSA
                     SGKEAAAQKGKEKDKEEKASKGPILLFVGPPGVGKTSIAKSIARAMGRKYVRVSLGGV
                     RDEADIRGHRRTYVGAMPGRIIQGMKQVGTKNPVFLLDEVDKLGVSFQGDPASALLEV
                     LDPAQNDSFTDHYLGVPFDLSEVLFVCTANFIQNIPGPLLDRMEVVEFSGYTEKEKRE
                     IARRYLIPRQVQENGLTEEQITITDEAVAETVTGYTRESGVRQLEREMGRLARKVARK
                     IAAREVERMDIDPAAVRELLGRPKVHPERAFREDQVGVATGMYYTPTGGDIMFVEAST
                     MKGKGELVLTGQLGDVMKESARAAWTYARSHAEQLGIPPECFERDVHIHVPAGAIPKD
                     GPSAGVTMATALVSTLSGRPARHDISMTGEITLAGRVLPIGGVKEKVLGAVRAGITEV
                     LLPKDNEADLEDLPEEVRDSIEFHFAEELGDVLALTLRGASYREGRLYFGDSQSADAG
                     IH"
     gene            complement(15836..>16130)
                     /locus_tag="VEL74_23660"
     CDS             complement(15836..>16130)
                     /locus_tag="VEL74_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016470092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HYG65600.1"
                     /translation="EDMLARTFYSHETPEGIKAWDRVRAAGYEPDIVGENIAAGHIEV
                     SEVMDAWMYSPGHRRNILDVRLRDIGIGVAVGSHDHRYQILWVQDFATLERAP"
BASE COUNT         2648 a         5426 c         5560 g         2496 t
ORIGIN      
        1 tctacttcgg cgacatcccg ctgatgacgg acaacggcac gttcatcatc aacggcaccg
       61 agcgggtcat cgtctcccag ctccatcgca gccccggcgt cttcttcacc aaggaaggcc
      121 cgcgcgcgta cctggcgaag atcatcccgt accgcggctc gtgggtggag ttcgagtacg
      181 accagaagga cgtgctgtcg gtccgcatcg accgcaagcg caagttccat ggcacggtct
      241 tcctgcgcgc cctcggcctc gagtcggacg agcagatcct gcgccagttc tacaccccgg
      301 tgggcctctc ctccgagggc ggcggcagct tcacgctgca cgtcacgccg aaggtgctgg
      361 agcccgagcg cgcgcgtgac cgccagaccc gcggccgccg cgaggcctac ccggtgttcg
      421 ccggcatcgt cctcggcaag gagcacatcg gcaagctcga caccggccag agcctggcct
      481 tcgaggtcgg cgccgccgac ctggagcgcg ccttcttcat cggcgacgtc gtcgacctcg
      541 acaccggcga ggtgatcttc gaggccaacg agctggtccc cgaggacatc gaggagcgca
      601 tggacggccg caaccacacc ccggtcgagg tcttcttccc cgactgggag ctgaccgggg
      661 cgacgctgtc caacacgctg gccaaggaca acacgcgcaa ctcgaaggaa gcgctgatcg
      721 agatctaccg gcgcatgcgc cccggcgatc ctccgaccct ggagagcgca cgttcgctct
      781 tctacggcat gttcttcgac gccaagcgct acgacttctc gcgggtcggc cgcttcaagt
      841 tcaatatcaa gctggacacc gaggtgccgg tcgaccagaa gacgctgtcc gcggacgact
      901 tcttccgggt catcgagtac ctgctgcgcc tgcagaagga cgtcggccgc gtcgacgaca
      961 tcgacaacct gggcaaccgc cgcgtgcggg ccgtcggcga gctgctggag aaccagttcc
     1021 gcatcggcct cgtgcgcatg gagcgggcga tcaaggagaa gatgtcggtc caccaggaca
     1081 tcgactcggc gatgccgcac gacctgatca actccaagcc ggtcatcgcg gcgatcaagg
     1141 agttcttcgg ctcgtcgcag ctgtcgcagt tcatggacca gacgaacccg ctttcggagg
     1201 tcacccacaa gcggcgcctg tccgccctcg gaccgggcgg cctgtcgcgt gagcgcgcgg
     1261 gcttcgaggt gcgcgacgtc cacgccacgc actacggccg gatctgcccg atcgagacgc
     1321 ctgaaggccc gaacatcggc ctgatctcgt cgctcgccac ctacgcgcgg atcaacgact
     1381 acggcttcat tgagagcccg tacaagaagg tcgagaacgg ccgcgtcctc gaccacttcc
     1441 ggatcatgaa ggtgggcgat ggcacgttcc gtctcggcca gatcgtcacc ggcgacgagc
     1501 tggaggcgga gaacgagcgg ctggagaagg ccggcaaacg ggcggtgtcc gtcgagccgc
     1561 acgccttcta cctctcggcc tgggaggagg agaagtacat cgtcgcgcag gccaacgccc
     1621 gcctcgacga gaagggctac ttcgtcaacg accgcatcat ctcgcgcgcc ggcggcgact
     1681 tcgtgaccgt ggagcgggac cgcatcgact acatggacat cagccccaag cagctggtgt
     1741 cggtggccgc ggcgctcatc ccgttcctcg agaacgacga cgccaaccgc gcgctgatgg
     1801 gctccaacat gcaacgccag gcggtgccgc tgctgcgcag cgaggcgccg gtggtgggca
     1861 ccggcctcga ggggatcgtc gcccaggact cgggcgcggt cgtcctcacc aagcgaggcg
     1921 gcgtggtcga ttcggtggac gccgagcgca tcatcgtccg cgtcgagggc gaggacctgg
     1981 agaccggcga gaccaaggac ttcggcgccg acatctacca gctcatcaag ttccggcgct
     2041 ccaaccagaa cacctgcatc acgcagaagc cggtggtcta cgaggggatg cgggtcaaga
     2101 agggggacgt cctcgccgac ggcccgtgca ccgagcgcgg tgagctggcc ctgggccgca
     2161 acgtgctggt cgccttcatg ccctggcgcg gctacaactt cgaggacgcg atcctgatct
     2221 ccgagaagct ggtgcgggag gactactaca cctccatcca catcgaggag ttcgagatcg
     2281 aggcgcgcga caccaagctc ggacccgagg agatcacgcg cgacatcccg aacgtctcgg
     2341 agtcggcgct caaggacctc gacgagagcg gcatcgtccg cctcggggcg tcggtcaagg
     2401 ccggcgacat cctggtgggc aaggtcacgc cgaagggcga gacccagctc acccccgagg
     2461 agaagctgct gcgcgccatc ttcggcgaga aggcgggcga cgtccgcgac gccagcctca
     2521 aggtgccccc gggcatcgag gggaccatcg tcgacgtcaa gatcttctcc cgcaaggggg
     2581 tcgagaagga ccagcgcgcc aaggacatcg agctggagga gatcggccgg ctcgagaaga
     2641 acatcaagga cgaggtccgc atcctcaacg aggagcgcaa caagaaggtc agcgacctcc
     2701 tcgtcggcaa gaaggtgtcg gcgccggtca agtcgcggac cggccaggag ctcctcaagg
     2761 cgggcgacgc catcaccctg gagaccctgg agcggctcac ccggcaggag atcctgcgcc
     2821 tgccggtgga cgagcggcgg ctcatcgacg cggcggagat cgtctacaag aagacggatt
     2881 cccacatcga ggtgctgcac cgcgtcaacg aggagcgcat cgggctcctg cagaagggcg
     2941 acgagctgcc cccgggcgtc atcaagctgg tcaaggtcta cgtcgccatg aagcgcaagc
     3001 tgcaggtggg cgacaagatg gccggccgtc acggcaacaa gggcgtgatc tcgcgcatcc
     3061 tccccgagga ggacatgccc tacctgcccg acggcacgcc ggtcgagatc gtgctcaacc
     3121 cgctgggcgt gccctcccgt atgaacgtcg gccagatcct cgagacgcac ctggggtggg
     3181 ccggcatggt gctgggggtg cgcttcgcga cgccggtctt cgacggcgcc cgcgagccgg
     3241 acatcaagag cctgctggaa gaggccaacc tgcccctgtc gggcaagacc gagctccatg
     3301 acggcatgac gggtgaggcg ttcgagcaga aggtcaccgt gggttacatc tacatgctca
     3361 agctgtccca tctggtggac gacaagatcc acgcgcggtc catcggaccg tactcgctga
     3421 tcacccagca gccgctgggc ggcaaggcgc agttcggcgg ccagcgcttc ggagagatgg
     3481 aggtctgggc cctggaggcg tacggcgccg cctacaccct ccaggagctg ctgaccgtca
     3541 agagcgacga cgtggaaggg cggagcaagg tgtacgaagc cattgtcaag ggcgacgtcc
     3601 ccgagccgcc gggtctgccg gagtctttca acgtgctcgt ccgcgagctc cagagcctgt
     3661 gtctcgacgt tgaactcctc aaggtttaag gaggaagaca ttgcaaccat tcagcagctt
     3721 tgacaagcca cagtcgctca aggatttcaa cgcgatcaag atctcgatcg cgtcgcccga
     3781 caagatccgg gcgtggagct acggcgaagt cacgaagccg gagaccatca actaccggac
     3841 cttcaagccg gagcgcgatg gcctgttctg cgccaagatc ttcggtccga tcacggactg
     3901 ggagtgcctg tgcggtaaat acaagcggat gaagcaccgt ggcgtcatct gcgacaagtg
     3961 cggcgtcgag gtcacccgtt cgcgggtgcg ccgcgagcgg atgggccaca tcgagctggc
     4021 ctgcccggtc tcccacgtct ggttcttcaa ggggctgccg agccgcatcg gccacctcct
     4081 ggacatcagc ctgcgcgacc tcgagcggat cctctacttc gagagctacg tcaccatcga
     4141 cccgggtgag acggagctca aggagcgcga gctcctgacc gaggagcggt tccgcgagat
     4201 gcgcgacaaa tacggcgacg agaccttcgc cgccaagatg ggcgccgaag cgatcaagga
     4261 gctcttggcg cgcatcgacg tcgacgagct ggccgaggag ctgcgcatca tcatgcgcac
     4321 cgagaccagc cagatccggc gcctcaaggc cgccaagcgg ctcaaggtgg tcgacgcctt
     4381 ccgcaggtcg ggccaccgcc cggagtggat gatcctggac gtgatcccgg tcatcccgcc
     4441 ggagctgcgg cccctcgtgc ccctggacgg cggccgcttc gccaccagcg acctgaacga
     4501 cctctaccgg cgcgtcatca accgcaacaa ccggttgaag aagctgctgg agctgcgcgc
     4561 ccccgacgtc atcgtccgca acgagaagcg gatgctgcag gaggcggtgg acgcgctgtt
     4621 cgacaacggc cgccgcggcc gcgtgctcaa gggctccaac aaccggccgc tcaagtcgct
     4681 gtcggacacc ctcaagggca agcaggggcg gttccgccag aacctgctcg gcaagcgcgt
     4741 cgactactcg ggccggtcgg tcatcgtcgt cggccccgag ctcaagctgt cgcagtgcgg
     4801 cctgccgaag aagatggcgc tcgagctctt caagccgttc atctacaacc ggctggagga
     4861 gaaggggctg gtcggcacga tcaaggccgc caaggagctg gtcgaggccg aggtggacga
     4921 ggtctgggac gcgctggagg aggtcatcca agaccatccg gtgctgctca accgcgcgcc
     4981 cacgctgcac cgtctgggca tccaggcgtt ccagccgacg ctgatcgagg gcaaggcgat
     5041 caagatccac ccgctggtct gcgccgcgtt caacgccgac ttcgacggcg accagatggc
     5101 ggtgcacgtg ccgctgtcgc cgcgcgccca gatcgagagc aaggtgctga tgctggcgtc
     5161 gaacaacatc ctgtcgcccg ctcacggccg gccgctggcg gtgccgtcgc aggacctggt
     5221 cctcggcggt tactacttga cgctggccaa gcccggggcc aagggcgagg gccgggtctt
     5281 cccggacttc gactcggtgc tcctggcgct cgaaggtggc gaggtggaga cgctgtcgac
     5341 catcaaggtg cgctacagcg gccgctgcat ccacctggag acgcagttgg acaagcagga
     5401 catcgtccac gccgaggtcg aggagctgga gaacaagctg atcgagacca ccgtcggccg
     5461 ggtcatcctc aacatgcacc tgccgaagga gatcccgtac atcaacggcc tgctcaagaa
     5521 gcgcggcctg caggacctgg tcggctactg cttcatcacc ctcggcaacg aggcgacggt
     5581 gcgcatgctc gacgaggtca aggaggtctc cttcctctac gccacccggg cgggcatctc
     5641 gttcggcgtc gacgacatgg tggtgccgag ccgcaagccg gagctgctgg acctggcgcg
     5701 gaaagaggtg gtcgagatcg agaaccagcg ctcggccggc gtcatcaccg ccggtgagcg
     5761 tcacaacaag atcatcgaca tctggcaccg caccaccgag aaggtgtccg aggagatgtt
     5821 ccgcgagatg cgggaggtgg agaagcgctc gggcgagttc aacccgatca acctcatggc
     5881 cgactccggc gcccgcggct cgcgcgagca ggtgcgccag ctcgccggta tgcgcggcct
     5941 catgtccaag ccctcgggcg aggtcatcga gaccccgatc acggcgaact tccgtgaggg
     6001 cctctcggtc ctccagtact tcatctccac ccacggcgcc cgcaagggcc tcgcggacac
     6061 ggcgttgaag acggcggact cgggctacct gacacggcgt ctggtcgacg tcgcccagga
     6121 cgtgatcatc aacgaggtgg actgcggcac gatcgacggc atcgtcgtgt cggcgatcat
     6181 ggagggcggc gagatcctcg agccgctgcg cgaccgcatc gtcggccgcg tcagccagga
     6241 ggacatctac gacccgatga ccggcgacat gctggtggag gtgaaccagg agatcaccga
     6301 gaccctgggc aactccgtgc aggcggccgg catcgagcgg gtgaggatcc gttccgtgct
     6361 cacctgcgag gcgcggcggg gcgtctgtgc ccgctgctac ggccgcaacc tggcgaccgg
     6421 ccgcatggtc gagatcggcg aggcggtcgg cgtcatcgcg gcgcagtcga tcggcgagcc
     6481 gggcacgcag ctcacgctgc ggacgttcca catcggtggt acggccgccc gaatcgccgc
     6541 ccagacgcag cgtcgcagca aggtcgaagg gatcgccgag ttcgagcgca tcaccaccgt
     6601 ccagacaccg gacggtaccc gcatcgtcaa gttcttgaac gtcaacacgg tcgagagcaa
     6661 gaagggggcg ctggtcgtca tcaaccgcag cggcaagctc ctcatcaccg acgagaaggg
     6721 ccgcgagcgg gagcgctacg ctctgacgta cggctcgcag ctcctggtgc acgaggggga
     6781 cgaggtgcag ccggggacgg agctggtgac ttgggacccg ttcacctacg ccatcctgtc
     6841 ggagatcggc ggccggatcg agttccagga catcgtcgag ggcgagaacg tccgagagga
     6901 gaccgacaag gtcaccggcc tgtcgcagaa gatcatcgtc gaggcttcgg ccaacgagaa
     6961 gcgggtcccc gccatcatca tccatggtga ggagcgggac aagcggtatc tcctcccctc
     7021 gggctcgcac ctcgtggtgc agaacgggca ggacgtccat cccggcgata cgctggtcaa
     7081 gatcccgcgc gagacgacca agaccaagga catcaccggc ggtctgccgc gggtcgtcga
     7141 gctcttcgag gcgcgcagcc ccaaggagcc ggcgatcatc acggagatcg acggcacggt
     7201 gcacctgggc gatgtccaca agggcatgcg caaggtcatc gtcgagagcg acgccggcga
     7261 gacccgcgag tacctggtgc cgcgctcgat ccacatcaac gtgcaggaga gcgagcgggt
     7321 gcgggccggc gatccgctga tcgacggcgc catcaacccc cacgacgtcc tccaggtgct
     7381 gggcgagaag gagctgcagc gctacctggt cgacaagatc caggaggtct accggtcgca
     7441 gagcgtcgcg atcaacgaca agcacatcga ggtgatcgtg cgccagatga tgcggtcggt
     7501 gaagatcgag gaggtcggcg acacggagtt cctgatcgac gagcaggtgg accgctggaa
     7561 gttccaggag gagaacgagc gcgtcatcgc cgccggcggc acccccgcca tcggccggcc
     7621 gctgctcctc ggcatcacca aggcgagcct gtcgaccgac agcttcatct cggccgccag
     7681 cttccaggag accacccggg tcctcaccga agccgcgatc agcggcaagg tggacggcct
     7741 gcgcggcttg aaggagaacg tgatcgtcgg ccgggtcatc cccgccggta cgggcatgga
     7801 ctactaccac gacttccatc tggccgagga cttcgtcgag ccggagccgg aggccgagga
     7861 ggagctcttc ttcgagttcg gcgaggagga gatccgcagc ctgccgcagg aggtcctcga
     7921 gggcgactag tcctctctcc catccgatcg ttcaaaaggg cggccgcgag gccgcccttt
     7981 ttctttatcc tctgcgctac agcatcctct gtctttcctg gcggacttcg attagacttc
     8041 cggcacaatc tatcgtttct gccatctccc aggagaggag gggaccatgg ccgcagttcc
     8101 ggacctcggc cggtacgtcg ccgtgcgcga gtacgggccc gccgatttcg agacggtgta
     8161 cgccatcgac tgtgccgcct tcgaggaggg gcggcggatg tcgcgcgaga agctgcggca
     8221 tcacctcggc accccgaaca cccgcgcgct ggtggcggag gagcggggga ccgtcgtggc
     8281 ctacgcgctg gcctgtgtga aggcccaggg cttgggatac ctcaaggtgc tggccgtggt
     8341 gccggggctc cagagccgcg gcatcggcag ccggctgatg gccgacatcg aggcctggct
     8401 ctgggagagc ggggccgggg ccatcatgct ggagaccgcc aacgacgaga ccagcgcccg
     8461 cgacttctac gagcggcatg gctacttcgt cctggaggtc ctggaccgct tctatgagga
     8521 cgggaccggc gccttccgca tgatgaaaga ccccgagatg tgatccgcgg ctacgcccgg
     8581 atgacttcga gcgccttctg gagatcgagc gggccgcctt cccgccatcc atcgcctatt
     8641 cgcgccgtga tctgcggcgc ctcttcgccg cccgccgctg ccgcaccctg gtcgccgaag
     8701 tcgacggggc cgtcgccggc ttcgtcaccg gctgccgcga gccgcgcggg atcgggcggg
     8761 tgaggagcct ggacgtggcc ccggagcggc agggggaggg gatcggcagc cgtctcttgg
     8821 cggccatcga ggagtggctc cgggagcagg gatctgcggc gctgctcctc gagaccgcgc
     8881 cgggcgcgcg cggcttctac gagcgccacg gctaccgaat cgacgagcgg ctcatcgatt
     8941 actaccgcga ggggctcgac gccttccgga tggtgaagat ggtgaagccc gtggaagacc
     9001 gcggggcgcc ggcggtgtta ccatcatggt gacacgcgaa aggaacgcca tgccgaaccg
     9061 gctcgtcctc gcccttatcc tcctcgtcct cccggccctg ccggtggatg ccgataccgt
     9121 ctatcttctc aacggacgca cgttcgaggg ggtgatcgcc gaggtcaccg actcgcaggt
     9181 ccggatccgc atgcagggcg gcgtgctgag cctgccgaag acgcaggtgc tgcgggtgga
     9241 ggagtccgac tccgactacg gcgagtacct gcggcgcaag ggaagcctgc agcgctccac
     9301 agctgcggcc ggcgactggc tggagctggc gcgctgggcc aaggcgcggg ggctccagca
     9361 ggcggcgcgc gaggcgggcc tcgaggccgc cgccatcgag cccggcctcg cggggctcga
     9421 gccgctgctg cgcggctacg gctacgtgct ggacaaggac ctcgaccgct ggatcccgta
     9481 cgccgactca atgcggcgca aggggttcgt ccaggcgaac ggccagtgga tcacccgcga
     9541 ggagtacgcc tcccgccagt cgcggcagcg cgacgaggag atcgcgcggc tcaaggcggc
     9601 gcgcgaggcg gccgacgcgg cgcgcatcgc ccgcgaggcc gagttggcgg tggccgagct
     9661 ggagctgcgg accgaaaagc tgcgctccgc ctccgcggct cctcaggtgc cctacggcac
     9721 ctacgggatg cccatgtacg tggtgcccgg ttatttcgcg ccgccgctgg tggtcgtccc
     9781 cggcggctgg tcccatggcg gcggctccca ccaccggccg cgcccctcgg cgccgtcggg
     9841 cggcggcggc caccgcccct cgaccggcgg cggccacaac cggagcagct acctccacca
     9901 gccgggcagc ctgatcccgg gccggctcgt gccgatgtcg acgaattgac cctccccagt
     9961 ctacgatgtc tcctcctcgc ccggtgcgcc ggggaggaga catctatgac acaatacggc
    10021 catcttcccc aggtagtcgg tgtggcgggc ggcggaccta cgatcctcgg aggtgagggt
    10081 ggtcgagcct gaactgtctt tgagagagct gtccctgttc gagggagtgg acgaggcacg
    10141 gttgcgtcag ctcgaatcgg cgctcgccag gaggagcttc gactcgaacg agctggtgtt
    10201 cagcaaggat gaccggtgtg acggcgtcta tgtcgtggca cgggggggcg tgaccatccg
    10261 tagcgaggtg gccggccagc cggtggagcg ggtgcgcgag ctgcggcagg gcgagctgtt
    10321 cggagagatc gagctgctgg aagggtcgcg gcgccacttc tcggcccgcg cggtggagcc
    10381 gacgaccgtc taccagatcc ctgccgaccc gttgtgggag ttcctgcgca gccatccgcc
    10441 ggtggaggcg cggctgcgct ccctggccat ccaccgccgg acgtcgcggc tgcgcctgct
    10501 gctctccccc cagacgcggc gacgggagcc gcggatctgg atcgaccgcc cggtcctgct
    10561 gacgctcggc gacggctcgc gctgcgaggc gcggctcgag aacctgtcga acagcggcgc
    10621 ctgcgtgagc ggcgcgcccg ccgcctggct gccgggagcg gaggtgagct tcgccctcgg
    10681 cgccggcaag atcccggagc tgctgcgcgt gcgctgcacc gtccgctggc gcgacggcga
    10741 cgccgtgggg ctcgccttcg acggtgtcgg ccaggcacac cgccgccgcg tcgaccagac
    10801 cgtccgccac ctggtgggct cccctccctg atcctgccgc atgcgggtcg tcgccgtcat
    10861 caaccgggga gcgggcacgg tgctccgcca gcagggtgtc gaccccgccg agctgcgccg
    10921 gatgttcgcc gaggccgggg ttgaggcgga gatcgagctc gtcgccggag aggagatcac
    10981 cgcggcggcg cgggcggccg tgtccggcgg cgccgaggcc gtgctcgccg gcggcggcga
    11041 cggcaccatc cgcgccgtgg ccggagcgct cgccggcggc acggtgccgc tgggggtgct
    11101 gccgctcggc acccgcaacc acttcgcgcg cgacctcggc atcccgatcg acctccccgg
    11161 ggcggtgggg gtggtggcgt cggggcaggt gcggcggctg gacctggggg aggtcaacgg
    11221 cgaggtcttc gtcaacaact cgatcctggg cttctacccg ccgctggtgc gggtccggga
    11281 ctggcagcgg cggaatctgt cacggggcaa ggggctggct acggcgatcg ccctgctacg
    11341 ggtcctgccg cgcctgccgg cgctccgcgt gcgcgtcaag gcggacggcc atgaggcggt
    11401 gcacgagacc cgcttcgtgt tcgtcggcaa caacgagtac cagatgagcc tgttcagcta
    11461 tggggcgcgc cagcggttcg acagcggcag cctctacctc tacgtcgcgc gcccccgggg
    11521 acggctcgga ctggtggggc tcgccctcct cggcctcgtg cgcgacgtca tccgcactcc
    11581 gcatttcgat cagtggatgc tgccggagtt caccatcgag acacggcagc gggcgctgcc
    11641 tgtctacctc gatggcgagg tcacactgat gcgcccgccc cttcactacc gcacgcggga
    11701 acgggcgctc tcggtgatcc tgccgtaagg agccggcggc gcttagcgtc cggtcgtcgg
    11761 attggtggcg gtgcgggtcg tgtcccggta gacgggctca cgcttccgca ggcccagcag
    11821 gccggccaga cccaggaggc cgatccagcc gagatccggc ccgtcgtcct cggtctgggt
    11881 agcataggtg ccgtcatcgg cggtctgggc ataggcgggc gcgaggccca gagtcaacag
    11941 gaacatgagg ctcaggatga aggcggtctt gcgcatgggg ttctctccta tagctagtca
    12001 ttccgtccag ctcggcatga gactggacca tgcaactgct tggaaccatt gcaagagcct
    12061 gaccaacttc ccgtccaggg cgggtgtgca atgatgggag ccatgaagag aggccgtcct
    12121 caagggcaaa aacggaccgt cctccagagt ggtcggtccg ggcccgcccg gagccggtgc
    12181 atggggccga aagcacgata gatcaaggat ttcggcgcgg tcccggacgc tggcatcgcg
    12241 gttgctgttg gggagcttcg gaccttgtgt cagaggagaa aaaaagaaaa tgacaaccaa
    12301 gacaattcgc acggccgtcg tgctgaccgg gctgctggtc gctgtctcgg caccttcctg
    12361 ggcgcagcgg cgcgcttatg agcgggatgg tgccttccgt ctacacgccg ggctgttcac
    12421 gcccgacggc gagtccgagt actgggacga caaggcggtg gacttcaccg gcgacgccca
    12481 ggacctggag aacgtctcca tcggcggcga ctatctccat agcctcggcc cgcggctcag
    12541 tctgctcttc tcgggcaact acttccaggg cgacaccacc cagtcctacg tcgacttcgt
    12601 cgacaacttc ggcgaccgca tccgtcacga cacgacgctg gagattgcgt cgctgacggc
    12661 cggcctcgtc cttcacctga ccgagccggg cgccgccatc cgtccctata tcggcgtggc
    12721 cggcgggctc tactcctgga gcgtggtgga ggagggcgac ttcatcgacg aggacaacct
    12781 cgagatcttc agcgcccggc tggagtccga cggcaacgtc ctcggctact acggcctcgc
    12841 gggcctcgag gtgccgatcg gccggaccgt ctcgctgttc ggggagggcc ggtggagcca
    12901 ggtcgaggac gagctgtccg gcgacctcga ggggttaggt gacatcgacc tctcgggccg
    12961 ccaactggcg gtcgggatct cctggagcct gtagcccttc tcaaccccag aaaaagagaa
    13021 cggccggtgc gcgagcaccg gccgtttttg tttggtcggg tccgatgggg gatcagtgga
    13081 tcccggcgtc ggcggactgg ctgtcgccga agtagagccg cccttcgcgg taggaggcgc
    13141 cgcggagggt cagggcgagg acgtcgccca gctcctcggc gaagtggaac tcgatggagt
    13201 cccgcacctc ttcgggcagg tcctcgaggt ccgcctcgtt gtccttgggc agcagcacct
    13261 cggtgatccc ggcccgcacg gcgcccagca ccttctcctt gacgccgccg atcggcagca
    13321 cccggccggc gagggtgatc tcaccggtca tggagatgtc gtgccgcgcc ggccgccccg
    13381 agagggtcga gaccagggcc gtcgccatgg tgacgccggc cgaggggccg tccttcggaa
    13441 tggcgcccgc cgggacgtgg atgtgcacat cgcgctcgaa gcactccggc gggatgccga
    13501 gctgctccgc gtgcgagcgg gcgtacgtcc aggcggcgcg ggccgactcc ttcatcacgt
    13561 cgccgagctg gccggtgagc accagctccc ccttgccctt catggtcgag gcctcgacga
    13621 acatgatgtc gccgccggtc ggcgtgtagt acatgccggt cgccacgccc acctggtcct
    13681 cgcggaacgc ccgctcgggg tggaccttgg gccggcccag cagctcgcgc acggcggccg
    13741 ggtcgatgtc catgcgctcc acctcgcgcg ccgcgatctt gcgcgccacc ttgcgggcga
    13801 ggcgccccat ctcgcgctcg agctggcgga cacccgactc ccgggtgtag ccggtgaccg
    13861 tctcggccac cgcctcgtcg gtgatggtga tctgctcctc ggtcaggccg ttctcctgca
    13921 cctgccgggg gatcagatag cgccgggcga tctcccgctt ctccttctcg gtgtagccgg
    13981 aaaactccac cacctccatg cggtcgagca gcggcccggg gatgttctgg atgaagttcg
    14041 ccgtgcagac gaacagcacc tcggacaggt cgaacggcac ccccaggtag tggtcggtga
    14101 agctgtcgtt ctgcgccggg tcgagcacct cgaggagcgc gctcgccggg tcgccctgga
    14161 acgagacgcc gagcttgtcg acctcgtcga ggaggaacac cgggttcttg gtgccgacct
    14221 gcttcatccc ctggatgatg cggccgggca tcgcgccgac gtaggtgcgc cggtggccgc
    14281 ggatgtcggc ctcgtcgcgg acgccgccga gggagacgcg gacgtacttg cggcccatgg
    14341 cgcgggcgat cgacttggcg atcgaggtct tgccgacgcc cggagggccg acgaagagga
    14401 ggatggggcc cttggaggcc ttctcctcct tgtccttctc cttgcccttc tgggcggcgg
    14461 cctccttgcc gctggccgag gtcgtcgccg cttgcacggc ctcctcgccg aagtggtcct
    14521 ccggatcgcc gatcacgtgg ctggcctcgg cggcttcggc gagctcggcg gcctccgcgg
    14581 ccgcgtcggc ctccgcttcg gcgcgccggc gcgccgcctg ctcctcacgg agctgacgca
    14641 cggcgaggaa ctccagcacg cggtccttga cgccgcccag ggcgtagtgg tcctcttcga
    14701 ggatcttcgc cgcctcgggc aggtcgaggt gctcctggct gcgctcgctc cacggcagct
    14761 cggcgatggt ctcgagatag gtgcggatca cctgcgactc catcgcctcc cggcccatgc
    14821 gggacaggcg gccgagctcg cggtcgacct ccttgcgcgc gtcgggcggc aggtcgagcc
    14881 cggcgagctt ctcgcgcagc tcctcgatct cggcgctgtt gtcgccctcg ccgagctcct
    14941 tctggatggc cttgagctgc tcgcgcagga acatctcgcg ctggcggtcc cccagctctt
    15001 cctgcacctt ggtcttgatg tcctcctgcg cctcgagcac ctcgagctgg cgctggacgt
    15061 ggaccagcac gcgccgcagc cgctcctcga ccgacagcgt ctcgagcagc atctggcgct
    15121 gcgccggggt gacgtcggtg taggcggcga cgaggtccgc gagacggctg gggtcctgga
    15181 ccccttcgag gacctgctgc acgacctcct cggggaggcc ggatttcttg cccagctcgg
    15241 ccgccctctc ccgcgcctcg cggaagagcg cgacgaacgc cgggtcgttc gcgttcaggg
    15301 gcttcatctc ctcggcttca cgcacgacgg cctggaggaa tccatccttc tcggtgatcc
    15361 gcatggcgat gccccggtgc tcgccgtgca gcaggagctg cattccggcc aggccgcgct
    15421 ggagctgccc gatgcgggcc acggtcccga tggtgtgcag cacctcgggc gtagggacct
    15481 gcgcctcttc gcggtgcgag acggcgaaga cgagccggtc cggcgtgttg agcgcggcct
    15541 cgatggcgcg cagggtgccg ggcctgccgg cattgatcgg cgccgtcacc ccggggaaaa
    15601 gcacgaactc ccgcagggga aggactggca gagtcaggat ttcagtcatc gtctctcctg
    15661 gatgggagat ccgcggccgc ccctcgcgac cccggccccc atcgaccgga tgccgggcca
    15721 accggccccg cttccgttac aaagcaaaac cggttccatc ccgtccacat tccgggatct
    15781 gcgagggatc ttagcatgaa tcgctcagac tgacggaggt ctgagtgttg tggactcaag
    15841 gagcgcgttc gagggtggcg aagtcctgga cccagaggat ctgataacga tggtcgtggc
    15901 tgcccacggc gacgccgatg ccgatgtccc tgaggcgcac gtccagaatg ttccggcggt
    15961 gtcccgggct gtacatccag gcgtccatga cctcggagac ctcgatgtgg ccggcggcga
    16021 tgttctcgcc gacgatgtcc ggctcgtagc cggcggcgcg gacccggtcc caggccttga
    16081 tcccctccgg ggtctcgtgg gagtagaagg tgcgcgccag catgtcctcg
//
LOCUS       DASPJI010000842        17142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_732393, whole genome shotgun sequence.
ACCESSION   DASPJI010000842 DASPJI010000000
VERSION     DASPJI010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17142)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17142
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732393"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1755)
                     /locus_tag="VEL74_23665"
     CDS             complement(<1..1755)
                     /locus_tag="VEL74_23665"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HYG65601.1"
                     /translation="MWKAGDRLTHRFHAELGPGIVREVRGRSVVVEFPEAAETGQVLS
                     FAAGSDALIPLVLAPGRRARLEGPDGTSEIVTIEDCEGGFCRLTDGREVAAYDLWPLP
                     AEVPPLERLVRGQVDGFEDFANRLDGIRLQRIRRAGGLGSFLGGRIRLFPHQLYAAER
                     ACQTNPVRWLLADEVGLGKTVEACLIMNRLIHTGRAGRTLVVAPETLVIQWLGELWRK
                     YHQVFVLLDEKRLADVARDYGESFNPFDVHRRAIVGLETLAARRRLTEQAVEAGIDLL
                     VVDEAHHLRRPAGHPGNEAYRAIAPIAAVGKNVLLLTATPLEDDAHGFFRLLQLLRPE
                     EFPEEESFDERLARREPLPPCTSATRRADIGGLPPRVPQPVDLDADGWSPMDRLVERL
                     RALPAGNALERRKKSERVARALSSPAALAAILDRQEREDAGLVATMTEAARQDPRLAW
                     LLRQAAGWQQAGEKTLVFVAHRETLETIREAIERQAAARVGIFHEDLAPERRDIEVAQ
                     FRLPDGPALLLSTEAGGEGRNFEFCRRLVLFDLPWNPAVVEQRIGRLDRIGRTRPTEI
                     VYFRPPAGLGRAVTALHEA"
     gene            complement(1847..2716)
                     /locus_tag="VEL74_23670"
     CDS             complement(1847..2716)
                     /locus_tag="VEL74_23670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65602.1"
                     /translation="MKTTQRTILSAALAVLLCPFAGQAAFAQSTQTPRIAELRIQVLE
                     AGSRRDLGYVNPGGSITVPEGSTIRLIMTALPAGNGRPLYPETEFTDPSNRGMRITRA
                     SEENANATIQVPSLNNGRERSETLRFQITENVNLPANQRTGSITLRVVPGQPAQTGSG
                     STGTAVPATGRSRELVTALYRGILLRDPDESGARNFISDVERNGFNGLVNAAQVIARS
                     EESRIQVYERENVCNQQRLLSLYKNLLGLGSNEIDSGQWDAYLRQLNSGDVAGVVQDM
                     MLSDRFRSYQRLS"
     gene            2932..3819
                     /locus_tag="VEL74_23675"
     CDS             2932..3819
                     /locus_tag="VEL74_23675"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HYG65603.1"
                     /translation="MIFSELDVHNRPETVLAYREAVERVIDEMRRRLSEELPLEELAE
                     IACISPFHFLRIFRSLTGLPPCQFLGALRLEKAKRLLLESDRSVTEVCLDVGYGSLGS
                     FTSRFTQLVGLSPSRFRQLSQAGGLPSLEDLPARLFPLFQGPGGVDVTGPLEASEGLI
                     FIGLFEHAIPQGRPLGCGIATGPGTFHLAAPGDGRFHLFALGMGWPREAGEILSDPSP
                     QQVGRAGPVTIRDGQVRGATALRMRAPDPLDPPLLLTLPLLIAEKLAALEAAERAARA
                     AAERRDSAAPTPALAAAAP"
     gene            4045..5121
                     /locus_tag="VEL74_23680"
     CDS             4045..5121
                     /locus_tag="VEL74_23680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65604.1"
                     /translation="MKRLWTLIAGAALALLVAAVPTFAVEPLYTPFDRVPAGFDYWQT
                     LGSGATAYSFANDPLPKGFFCKGSRAFAGTLKFEGVPLRTIPQGILGATDTIIERLDD
                     AVFDDQGVGMTRIQIRELQLVSSTPITTPCGTWRAVAQLAPGEQPITPMRLTRRNQFG
                     GDYQADLWIKVEIDFIRESDGEARTVNRTLHLPTAKTTAYACWSRHATATPATPTNPT
                     GPTGPTGPTGTSGTTTSMGSPSTSASSTTTSVYVQQPGTTTRVRVYDFDSNGCVVGTR
                     CDSNGVCLPVYSWHEAFDDEDHFVFTPCDLGYEQFCDEEDEEAYLAQLKDLRRLGIID
                     ADPEVVLERQRHPRGQRLLPQIRN"
     gene            5295..6086
                     /locus_tag="VEL74_23685"
     CDS             5295..6086
                     /locus_tag="VEL74_23685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65605.1"
                     /translation="MAKMRFAILGAVALLAALALPAAANDVIYSGIDTWRTPADGSTF
                     VDFSHEPLPAGFFCGKSAAFTGKVPFKGVPIVTEGGSLGPTDTIVERLDDVTLDENGV
                     GTTRVQLRALQFESIAPVKTACGKFNVRVHLDGEQPITEMRIVKENETGGRFFAHIGL
                     RARVIFTPVRGQARRPMEVVREVRFQPSSRAPWSLRPSDKAVQRMGFVQVDTDADGTP
                     DAFLPGTSNFAAGASMDKSIVYYVCHDDPNCGVHCQQVVGPTIEY"
     gene            6344..7348
                     /locus_tag="VEL74_23690"
     CDS             6344..7348
                     /locus_tag="VEL74_23690"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HYG65606.1"
                     /translation="MQFPFLQRLRPRFGKVWLWLALLVVPLFVLRACVVTYVPPDQIG
                     VRQVAYGPGKGLQKKPVYPGYQRELTSYERIHTFPRSIQAVEFTDNSQEAGAGNRQLP
                     AVRVPTVDGYPVDVDVTVLYRIAEPYQVASRFGFGKNYEEAVVTRFTDPLVKQHLGQL
                     LAEEFYGEARLAKVHALKAALTARFKENGLHLVDVLVRQYDYPETFQALTEQKKIQDQ
                     SVLANREFTKQAEVQTRLNQVLAEGKNLMNVRTSEFNAQISGINADKDLYERQKRAEA
                     DLLVRSAEAEGSELINRALEGAGSAKLLRLRRGLALLNSIKGPIYISEDPTDLGRIIQ
                     "
     gene            7366..8397
                     /locus_tag="VEL74_23695"
     CDS             7366..8397
                     /locus_tag="VEL74_23695"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HYG65607.1"
                     /translation="MKAILIPILCLPLLAAGLAGCTPHRTEATEVGVKFNKLTAKYEI
                     KEAGATHFFMPVLNDWKTFDISTRNLVMSTAEKASDRHGKDELRFKTKDGNDIETDVT
                     VRWRIDPTRVEDIWRYAAPETDSVEDTLVRPLARTYVRDVLNRLESEDYYNPTDRFQA
                     ASDATVRLANHLKQYGIVVEQVLLGDFSFKPEYQRLINARKEAEKTAEKLEAEILATQ
                     EVNQANLQSKVAELTEQLTRAQGEFEQAKRAADAYLVRRQQEAQAILAEKRAQAEGIR
                     KERDALNGSAGDAYVNLQLIEALQKKEIRQMPKLPGGNMIIDGNKLLEQLGVVRYEQN
                     RPVEQTPRQ"
     gene            8407..9078
                     /locus_tag="VEL74_23700"
     CDS             8407..9078
                     /locus_tag="VEL74_23700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65608.1"
                     /translation="MLNLAGSSYDFGKVLFAVLQECEHRRRALAPAEAEQGLMEIARD
                     KLAEIRESYQEAGGSRPYWEDLEHEVLNTALPQYIPRAVEQTRLEGAGYDVFRKGDPA
                     ARVVFGLLGLILGALLILLPPQLIVERGVAFLLAAVGVLYPEIKKGYHDYRFSKFLNR
                     LIVKAEKYQNDRRLHYVSNARLEEELRSLETPAEKPREGATVHPFPKEPEGSGPRRGG
                     RRVKG"
     gene            complement(9083..9322)
                     /locus_tag="VEL74_23705"
     CDS             complement(9083..9322)
                     /locus_tag="VEL74_23705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HYG65609.1"
                     /translation="MLDLRYSLVIEATDDPSFFGFYSPDLEGFTGIGHSIEDCLYKAR
                     WGMKEHVELLEEQGMPVPPRAPKPTVLIQNEERAA"
     gene            complement(9334..9525)
                     /locus_tag="VEL74_23710"
     CDS             complement(9334..9525)
                     /locus_tag="VEL74_23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HYG65610.1"
                     /translation="MKFSELVRTLEAEGFRLVKEKGSVRYYGKRGLDRLIRVDFHGSR
                     EVPRGTCQAILKAAGISKK"
     gene            10315..10437
                     /locus_tag="VEL74_23715"
     CDS             10315..10437
                     /locus_tag="VEL74_23715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65611.1"
                     /translation="MPAVGLAVPVIGLGRPVIGAARPVIGLAVPVAGEALPTAG"
     gene            10539..11858
                     /locus_tag="VEL74_23720"
     CDS             10539..11858
                     /locus_tag="VEL74_23720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65612.1"
                     /translation="MDFDTRKKYYNLCKPTEPLAPGDPRNLPIDELDGAVRGWHWVDR
                     FAKKIGMVEEPVLELFTGLPGSGKSTELRRLSARLSDKKGAHLLPVLIDAEEVIDLTN
                     PIDVPDILAAILHETEKCVLIHEGRNSDQALQEGYLTRFWHWLTTTDVAIGQAGFSVP
                     GGGPNLVAEMKTRPSLRKRVRETVAAHLSGFLGEVEDEMSLLDRRARDLGYRGLVVIF
                     DSLEKLRGISTNWKDVLDSAEEIFAANAPHLRLPVHVIYTVPAALVARARIANVEFMP
                     MIKLQTREGEPFEPGMEAARELIRRRIPDDVLGELFGPDAEVRLAKLIRWSGGYPREI
                     VRLLREVLLLSEEPVPDEDVERILGEVRSSYRMMVSSDAFEWLARVSVERFLTLENDD
                     HRQVADRMLQNNAVLRYLNQESWFDLHPALYEIPGLQKVIARMKSGG"
     gene            11980..13590
                     /locus_tag="VEL74_23725"
     CDS             11980..13590
                     /locus_tag="VEL74_23725"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG65613.1"
                     /translation="MAQAAFRLIEEETGSAVHRLRPEPFYLREPVPYEHLISAVLEPL
                     VWPQFEGEDVPRVIVIDASDAAKRDEGSWRQLFRRMNERRNVIAQRVPGTLLLCVTPS
                     LHLAFMQEAPDFWSIRGAEVTLEPPPAPAEPLGARPEVEHERREERAPSVETEPERLR
                     AAVAVARKRMEELPEDASALRALMIQLDRLGRYEVEWGSLERAQTAYEEAVDLARWRM
                     ELAPDRPEWLRDLSVSLNDLGDVLVSRGSLERAQESYEESLGVMRRLLEQDPDRPEWL
                     RDLSVSLIKVGDILRSRGFLERAQESYEESLSVMRRLLEQDPDRPEWLRDLSVSQGNV
                     GDVLRSRGSLESAQSSYEESLGVTRRLLEQDPDRPEWLRDLSVSLNKVGNVLLARGSL
                     ERAQSSYEESLGVMRCLLEQDPDRPEWLRDLSVSLERVGDVLRSRGSLERAQSSYEES
                     LGVRRRLLEQDPDRPEWLRDLAVSLLRIGTIHEARGEREQAILLYEQALQIVRDLQAR
                     FPYQPLYGEDVAWLERRLKGLRGEKPPS"
     gene            14049..14432
                     /locus_tag="VEL74_23730"
     CDS             14049..14432
                     /locus_tag="VEL74_23730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65614.1"
                     /translation="MESLPDGSFGFFRRGESPERGYRPAMRFVRRSDALLALAMIEAR
                     REPEYRLLPAPDGHGYLVQSRSAWGEVVGGLVIFDDDLIARVSWAEALMRSPEALALF
                     LEACGKVVLERAGAILEERVRAMAP"
     gene            14496..15170
                     /locus_tag="VEL74_23735"
     CDS             14496..15170
                     /locus_tag="VEL74_23735"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYG65615.1"
                     /translation="MELLYTSHVPAGDGPHPTVILLHGWGANAHDLLGLAPMLHGGRA
                     LVLCPQGQVEFSIGGGMAGYGWFPLVPGAPPDPQAFLRAADSLRGFVDKALERYPIDP
                     KRLVVAGFSQGGLMAYELGLRQPARFAGIAALSSWFPQPLAENLPHLPEHQDFPVLMV
                     HGTQDTMLEVDRAREGRELLRPFGVSITYREFDMGHEIRPDALKVILRWLDEKAFGRT
                     EGAAGG"
     gene            complement(15167..15364)
                     /locus_tag="VEL74_23740"
     CDS             complement(15167..15364)
                     /locus_tag="VEL74_23740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65616.1"
                     /translation="MTRREALHRLIDDLPEQEIPTAARVLEALRATADPVLRTLFSAP
                     LDDEEDDDDFDGGLTEARSEA"
     gene            15462..>17142
                     /locus_tag="VEL74_23745"
     CDS             15462..>17142
                     /locus_tag="VEL74_23745"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HYG65617.1"
                     /translation="MPDQQPSVDAANVFQVHFLDMGSKKYGDCIVCLIGDKRILIDGG
                     HLGDQRRGHPDYPSIPEQLAEILGQPAPWRFDLLVVTHAHVDHIGCLPNLVSTGQIVA
                     DWVLAADEELGWGDVEGVDAPAFEDAPEPVRQAFALLREERPAFASREEMEAFASDAA
                     RMRPTYAAMLDALDRGGAKVVRFGRDDPADLVAALRPETGLEILGPTPEHLLACARQI
                     AALGRDAADVIVERSRRADHPLDLVEAYEALLMGSLYRDSGEFELGNAINDQSLVLAF
                     GRGGRKVLLTGDMQLADPLVDGLEESMAELRRHIARNGPYSLVKLPHHGARNGVDAEL
                     LAETGAPLFVISGGQGSAKHPHPSTLEVLETAQGIAWYRTDRNGWISVDLATAEIEAE
                     WVRGEPNDRGRNPEARDFLDEPGPALPPPAEPPAPPSPEFTRSVVRTPDGEIVEVLAR
                     IPHRRTKVVITIEVEPSEEPSGEIAAAGRRESPAAGAGRPAADRVTPRPAGGQGAALR
                     LGGGRELPPLLFATDPDALANNIGREEARAVCKALEDSGQTVVFDGFRKMSS"
BASE COUNT         2644 a         5867 c         5916 g         2715 t
ORIGIN      
        1 cgcctcgtgc agcgcggtga ccgccctccc cagccccgcg ggcggccgga agtagacgat
       61 ctcggtcggc cgggtccgcc cgatgcggtc gagacggccg atgcgctgct cgaccaccgc
      121 cgggttccag ggcaggtcga agaggacgag ccgccggcag aactcgaagt tcctcccctc
      181 gccgcccgcc tcggtggaca gcaggagcgc cggcccgtcc ggcagccgga actgcgccac
      241 ctcgatgtcc cgccgctcgg gggcgaggtc ctcgtggaag atccccaccc gcgccgcggc
      301 ctgccgctcg atcgcctcgc ggatcgtctc cagcgtctcg cggtgggcga cgaagaccag
      361 ggtcttctcc ccggcctgct gccagcccgc cgcctggcgc agcagccagg cgagccgcgg
      421 gtcctgccgc gccgcctcgg tcatcgtggc gacgagcccg gcgtcctcgc gctcctggcg
      481 gtcgaggatg gcggcgaggg cggcggggga ggagagggcg cgggccaccc gttcggactt
      541 cttgcggcgc tcgagggcgt tgccggcggg cagcgcgcgc agccgctcca ccagccggtc
      601 catgggggac cagccgtccg cgtccaggtc caccggctgg gggacccggg ggggcaggcc
      661 gccgatgtcg gcgcggcggg tggcgctggt gcacggcggc agcggctcgc gccgggcgag
      721 ccgctcgtcg aacgactcct cttccgggaa ttcctcgggc cgcaggagct ggagcagccg
      781 gaagaagccg tgggcgtcgt cctccagcgg cgtggcggtg aggagcagga cgttcttccc
      841 cacggcggcg atgggcgcga tggcgcggta ggcctcgttg ccgggatggc ccgccgggcg
      901 ccgcaggtgg tgcgcctcgt ccaccaccag caggtcgatg ccggcctcga ccgcctgctc
      961 ggtgaggcgc cgccgcgcgg cgagggtctc gagcccgacg atggcgcggc ggtgcacgtc
     1021 gaaggggttg aagctctcgc cgtagtcgcg ggcgacgtcc gccagccgct tctcgtcgag
     1081 caggacgaac acctggtggt atttgcgcca cagctcgccg agccactgga tcaccagggt
     1141 ctcgggggcg acgaccaggg tgcgcccggc gcgcccggtg tggatcagcc ggttcatgat
     1201 gaggcaggcc tcgaccgtct tgcccagccc cacctcgtcg gccagcagcc agcgcacggg
     1261 gttggtctgg caggcgcgct cggcggcgta gagctggtgg gggaagaggc ggatgcggcc
     1321 gccgaggaac gagccgaggc cgccggcgcg gcggatgcgc tgcagccgga tgccgtcgag
     1381 ccggttggcg aagtcctcga agccgtccac ctggccgcgg actaggcgct ccagcggcgg
     1441 cacctcggca ggcagcggcc agaggtcgta ggcggcgacc tcccggccgt ccgtgagacg
     1501 gcagaagccg ccctcgcagt cctcgatggt gacgatctcg ctggtaccgt ccggaccctc
     1561 caggcgcgcg cggcggccgg gggcgaggac gagggggatg agggcatccg agccggcggc
     1621 gaagctcagc acctggccgg tctcggccgc ctcggggaac tccacgacga cgctgcggcc
     1681 acggacctcg cggacgatcc cggggccgag ctcggcatgg aagcgatggg tgagtcgatc
     1741 tcctgccttc cacatagggg gcgacattgt acgggagcgt gggccaatga aaaggccggg
     1801 tccggagacc cggcctctgg agaggacgct gctggtggcc gttcgctcag ctcagccgct
     1861 gataggaacg gaagcggtcc gacagcatca tgtcctggac tacgccggcg acgtcgccgg
     1921 agttgagctg ccgcaggtag gcgtcccact ggccgctgtc gatctcgttg gagccgagcc
     1981 ccagcaggtt cttgtagagc gagagcagcc gctgctggtt gcagacgttc tcccgctcgt
     2041 agacctggat gcggctctcc tcgctgcggg cgatcacctg ggcggcgttg accaggccgt
     2101 tgaagccgtt ccgctccacg tcgctgatga agttgcgggc gccggactcg tccgggtcac
     2161 gcagcaggat gccccggtag agggcggtca ccagctcgcg cgagcggccg gtggccggca
     2221 cggcggtccc ggtcgatccc gatcccgtct gggcgggctg gccgggcacc acgcggaggg
     2281 tgatggagcc ggtccgctgg ttggccggca ggttgacgtt ctcggtgatc tggaagcgca
     2341 gggtctccga ccgctcccgg ccgttgttga ggctcggcac ctggatcgtg gcgttggcgt
     2401 tctcctcgct ggcgcgggtg atgcgcatcc cgcggttcga cgggtcggtg aactccgtct
     2461 ccggatagag cggcctgccg ttgcccgccg gcagggcggt catgatgagg cggatggtcg
     2521 agccttcggg caccgtgatg gagccgcccg gattgacgta tcccaggtca cggcgcgagc
     2581 cggcctcgag gacctggatg cgcagctcgg cgatgcgcgg cgtctgcgtg ctctgcgcga
     2641 aagcggcctg gccggcgaac gggcaaagaa gcactgccag ggcggccgaa agaatcgtgc
     2701 gttgtgtcgt cttcataaga cgctcctcct catcgttgtc tggcgaaggc caaaaggacg
     2761 gctgccctcg ccccggatca cgcgcgtagc gaaccgggcg ccgttaccaa tgccgtcccc
     2821 tctcaggagt agcaagaggg gtgccacgcc ggaggctcgc gtttccttcc cttctgcacc
     2881 ttctcaagta ggaggatgtc tgcgggatct tctcccgcgg gccctggccg cgtgatcttc
     2941 tcggaattgg atgtccacaa ccgtcccgag accgtgctgg cctatcgcga ggcggtcgag
     3001 cgggtgatcg acgagatgcg ccgccgtctc agcgaggagc tcccgctgga ggagctggcg
     3061 gagatcgcct gcatcagccc cttccacttc ctgcgcatct tccgcagcct gaccggcctg
     3121 cccccctgtc agttcctggg cgccctgcgg ctggagaagg ccaagcggct gctcctggag
     3181 agcgaccgga gcgtcaccga ggtctgcctc gacgtcgggt acggcagcct ggggagcttc
     3241 accagccgct tcacccagct cgtggggctc tcgccctccc gcttccgcca gctctcccag
     3301 gccggcggcc tgccgagcct ggaggatctg ccggcgcgtc tcttcccgct gttccagggg
     3361 ccgggaggcg tcgacgtcac cggcccgctg gaggcgtccg aggggctgat cttcatcggc
     3421 ctcttcgagc acgccatccc gcaggggcgt ccgctgggct gtggcatcgc cacgggaccg
     3481 ggcacgttcc acctggcggc gccgggcgac ggccggttcc atctgttcgc cctcggcatg
     3541 ggatggccgc gggaggcggg ggagatcctg tccgacccga gcccccagca ggtcggccgc
     3601 gccggcccgg tgaccatccg cgacggccag gtccggggcg ccaccgccct gcggatgcgc
     3661 gcgcccgatc ccctggatcc gccgctgctc ctgaccctgc cgctgctgat cgccgagaag
     3721 ctggccgccc tggaggcggc cgagcgggcc gccagggccg ccgccgaacg gcgggatagc
     3781 gcggcgccga caccggccct ggccgcggcg gcgccgtagc cggcaccgat ttccggttcc
     3841 tcccgcccgg ctcttccggg cgcctctgcg gagtgcgcgc tttttacgcc ttttggcgtt
     3901 gaatctctcc tgaagtccgc aatctggaag aagtttttct ctgcgcgact ccttaatctt
     3961 ctatacgtga gagcggagac ggcgcgggga tcgcggcagc tccgtgccgg atcgtgaatg
     4021 ccgcacgttc aggaggagat cgagatgaag agattgtgga ctttgatcgc cggagcagcg
     4081 ctggccctgc tggtggccgc ggttcccacg ttcgccgtcg aaccgctgta cacgccgttc
     4141 gaccgcgttc ccgcgggctt cgactactgg cagaccctgg ggtcgggggc gaccgcctac
     4201 agcttcgcca acgatccgct gccgaagggc ttcttctgca agggctcccg ggccttcgcc
     4261 gggaccctca agttcgaggg cgtgccgctg cgcaccatcc cccaggggat cctgggcgcc
     4321 accgacacga tcatcgagcg cctcgacgac gcggtcttcg acgaccaggg cgtcggcatg
     4381 acccgcatcc agatccgcga gctgcaactg gtctccagca cgccgatcac gaccccttgc
     4441 ggcacgtgga gggcggtcgc ccaactcgcc ccgggcgagc agccgatcac ccccatgcgg
     4501 ctgacccgcc gcaaccagtt cggcggcgac tatcaggccg acctctggat caaggtggag
     4561 atcgacttca tccgcgagtc ggacggtgag gcccgcacgg tgaaccgtac cctccatctg
     4621 ccgacggcga agaccaccgc ctacgcctgc tggtcccggc atgccaccgc cactcccgcg
     4681 accccgacca acccgaccgg cccgaccggc ccgaccggcc caaccggcac gtccggtacg
     4741 acgaccagca tgggcagtcc gtcgacctcg gcaagctcca ccacgaccag cgtctacgtc
     4801 cagcagcccg gcaccacgac ccgggtgcgg gtctacgact tcgactccaa cggctgcgtg
     4861 gtcggcaccc ggtgcgacag caacggcgtc tgcctgccgg tctactcctg gcacgaggcg
     4921 ttcgacgacg aggaccactt cgtcttcacc ccctgcgacc tcggctacga gcagttctgt
     4981 gacgaggagg acgaggaggc ctatctggcc cagctcaagg atctccgccg cctcggcatc
     5041 atcgacgccg accccgaggt agtcctcgag cgccagcgcc acccgcgcgg ccagcggctg
     5101 ctcccccaga tccggaacta ggtccctccg gagccttcac tcccggcccg ggcgcccgcc
     5161 cgggccgttt tctgaaagat agcaatttcg aagaagtaat caggctgttc gaggtctagc
     5221 atcgtcctca gatcttccgg atgcaccggt agacccttgt ctcattcctc tatagaggaa
     5281 gaggaggacc gaccatggcc aagatgcgct ttgccatcct cggagccgtc gccctgctgg
     5341 cggcgctcgc cctgcctgcc gccgccaacg atgtcatcta cagtgggatc gacacctggc
     5401 ggacgccggc cgacggctcg accttcgtcg atttctccca cgagccgctc ccggccggct
     5461 tcttctgcgg caagtcggcg gccttcaccg gcaaggtgcc gttcaagggc gtgccgattg
     5521 tgacggaggg aggctcgttg ggacccaccg ataccatcgt cgagcgcctc gatgacgtga
     5581 ccctggacga gaacggcgtg ggcaccacgc gggtacagct ccgggcgctg cagttcgaga
     5641 gcatcgcccc ggtcaagacg gcctgcggca agttcaacgt ccgcgtccac ctcgacggcg
     5701 agcagccgat caccgaaatg cggatcgtca aggagaacga gaccggcggc cggttcttcg
     5761 ctcacatcgg cctccgcgcc cgggtgatct tcacgccggt gcgcggccaa gcccgtcgcc
     5821 ccatggaggt cgtccgcgag gtccgcttcc agcccagctc ccgcgcgccc tggagcctga
     5881 ggccgtcgga caaggccgtc cagaggatgg gcttcgtgca ggtggacacc gacgccgacg
     5941 gcactccgga cgccttcctc cccggcacct cgaacttcgc cgctggcgcc tcgatggaca
     6001 aatcaatagt ttattatgtc tgccacgacg acccgaactg cggcgtccac tgccagcagg
     6061 tagtcggtcc gaccatagaa tattaggacc cgaactgcca atccggcccg ggcccccgcc
     6121 cgggccggcc ctccctctcc cacccccatg ctaagatccc cccaggtctc ccgataggaa
     6181 ccccgtcgcc ctgtgtgggt gaggtgtctc tcccggggcc ttccgcgacg gcaggagcag
     6241 cagcttgcaa ctcccgggtc cttccttcgt agctctgtcc agaaggacac tcttttgaga
     6301 acccaaaacc atgtttgaga acgcgcaatc tcccagattc cgtgtgcagt tccctttcct
     6361 ccagcgcctg cgcccccgct tcggcaaggt gtggctctgg ctggccctcc tcgtggtgcc
     6421 gctgttcgtg ctgcgggcct gcgtcgtgac ctacgtgccg cccgaccaga tcggcgtgcg
     6481 ccaggtggcc tacggcccgg gcaagggcct gcagaagaag ccggtctatc ccgggtatca
     6541 gcgggagctc accagctacg agcggatcca caccttcccg cgctccatcc aggcggtgga
     6601 gttcaccgac aactcccagg aggcgggagc gggcaaccgc cagctcccgg cggtgcgtgt
     6661 gcccacggtc gacggctacc ccgtggacgt ggacgtcacc gtcctctacc gcatcgccga
     6721 gccttatcag gtcgcgtccc ggttcggctt cggcaagaac tacgaggagg cggtggtcac
     6781 ccgcttcacc gatccgctgg tcaagcagca cctggggcag ctcctggccg aggagttcta
     6841 cggcgaggcg cgcctcgcca aggtgcacgc cctcaaggcg gcgctcaccg cgcgtttcaa
     6901 ggagaacggc ctgcatctgg tggacgtgct ggtccgccag tacgactatc cggagacgtt
     6961 ccaggccctg accgagcaga agaagatcca ggaccagtcg gtgctcgcca accgcgagtt
     7021 caccaagcag gccgaggtcc agacgcggct caaccaggtg ctcgccgagg gcaagaacct
     7081 gatgaacgtc cggacctcgg agttcaacgc ccagatctcc ggcatcaacg ccgacaagga
     7141 cctctacgag cgccagaagc gggccgaggc cgacctcctg gtgcggtcgg cggaggccga
     7201 gggcagcgag ctcatcaacc gcgcgctgga aggcgccggc tcggccaagc tcctccggct
     7261 gcgccgcggg ctcgccctgc tcaacagcat caagggcccc atctacatca gcgaagaccc
     7321 caccgacctg gggcggatca tccagtgagg caggagacca cggccatgaa agcaatcctg
     7381 atcccgatcc tctgtctgcc cctcctcgcc gcagggctcg ccggctgcac gccgcaccgc
     7441 accgaggcga ccgaagtggg cgtcaagttc aacaagctga ccgccaagta cgagatcaaa
     7501 gaggccggcg ccacccattt cttcatgccg gtcctcaacg actggaagac gttcgacatc
     7561 tcgacgcgca acctggtgat gagcacggcc gaaaaggcga gcgaccgcca cggcaaggac
     7621 gagctgcgct tcaagaccaa ggacgggaac gacatcgaga ccgacgtcac ggtccgctgg
     7681 cgtatcgacc cgacgcgggt ggaagacatc tggagatacg cggcgccgga gaccgactcg
     7741 gtggaggata cgctggtgcg gccgctggcg cggacctacg tccgggacgt cctcaaccgc
     7801 ctggagtccg aggactacta caacccgacg gatcgcttcc aggcggcgag cgacgccacc
     7861 gtgcgcctgg cgaaccacct gaagcagtac gggatcgtgg tcgagcaggt gctgctgggg
     7921 gacttctcgt tcaagcccga gtaccagcgc ctgatcaacg cccgcaagga ggcggagaag
     7981 acggcggaga agctggaggc cgagatcctg gcgacgcagg aggtcaacca ggcgaacctg
     8041 cagtccaagg tcgccgagct gaccgagcag ctcacccgcg cgcagggcga attcgagcag
     8101 gccaagcggg ccgccgacgc ctacctggtc cgccgccagc aggaggcgca ggcgatcctg
     8161 gccgagaagc gggcgcaggc cgagggcatc cgcaaggagc gcgacgcgct gaacggcagc
     8221 gccggcgacg cctacgtcaa cctgcagctc atcgaggcct tgcagaaaaa ggagatccgg
     8281 cagatgccca agctgccggg cggcaacatg atcatcgatg gcaacaagct cctggagcaa
     8341 ctcggggtgg tccgctacga gcagaaccgg cccgtcgagc agaccccgag gcagtaggat
     8401 gaggccatgc tgaacctggc gggctcatcg tacgacttcg gaaaagtcct cttcgccgtg
     8461 ctccaggagt gcgagcaccg gcggcgggcg ctcgcgcccg cggaggccga gcaggggctg
     8521 atggagatcg cccgcgacaa gctcgcggag atccgggaga gctaccagga ggcgggcggc
     8581 tcccgtccct attgggagga tctggagcac gaggtgctga acaccgccct gccgcagtac
     8641 atcccccggg cggtcgagca gacgcgcctg gaaggggctg gctacgacgt cttccgcaag
     8701 ggggacccgg cggcccgggt ggtgttcggc ctcctcggcc tgatcctggg agcgctgctc
     8761 atcctgctgc ccccgcagct catcgtggag agaggcgtcg ccttcctcct ggcggcggtc
     8821 ggcgtcctgt accccgagat caagaagggg tatcacgact accggttctc caagttcctc
     8881 aaccggctga tcgtcaaggc cgagaagtac cagaacgacc gccgcctcca ctacgtgagc
     8941 aacgcccgcc tggaggagga gctgcggtcg ctggagacgc cggccgaaaa gccccgcgag
     9001 ggggcgacgg tccacccctt cccgaaggag ccggaaggct ccggtccgcg gcggggtggg
     9061 aggcgggtga aggggtaggg cctcaggcgg cccgttcctc gttctggatg agcacggtcg
     9121 gtttcggcgc gcgaggcggc accggcatgc cctgctcttc cagaagctcg acatgctcct
     9181 tcattcccca ccgcgccttg tacaggcagt cctcaatgga gtggccgatg cccgtaaatc
     9241 cttccaggtc cggcgaatag aagccgaaga agctgggatc gtcggtggct tcgatgacca
     9301 gggagtatct gagatcgagc atgggagctc ctttcacttc ttgctgatgc ctgccgcctt
     9361 gaggatagcc tggcacgtgc cgcgaggaac ctcccgcgag ccgtggaagt ccacccggat
     9421 taaacgatcg agaccacgct tgccgtagta ccggacagaa cctttctctt tgacgagcct
     9481 gaacccttcg gcttccaaag tgcggaccag ctcgctgaac ttcatcgagc gctggagcct
     9541 gaggtcgagc ggcgagcgtc catggaacaa atcctaccat cgaggccggc accccccgcc
     9601 gcccggggtt cccgcccacc cccacgccac cccgggctac cttcgtacgt ccctccggga
     9661 ctgaggcgcg aaggacggtg gtgcctccca cacacgtcaa cttggggccc cgacagggcg
     9721 gtggtgcctc cccccctggg cggaggggcg ggagggagcg ggggaggtga aggagggggg
     9781 tttttctccc ggggcagccg ctgccccacg agcatggccg ccctacccgg cgaaatggcc
     9841 gtcatccccc gcgccgcgac cgtcataccc agggtaaacc ctccgtaccc gcggaaatga
     9901 tcgccgtctg ccgggagatg ttctccatac ccggtggaag atcgccgtac ccgcggagat
     9961 ggtgacccta ccctgggtat ggccgccata ccccgggtag ggaggccgta cccacgggga
    10021 tgaacgccat ctccccaggg atgatggacg cggaaacccc tccggtcttc gcctgggcct
    10081 ccttcatctc tatcttgcgg cgaagggtcc ctcccacctg ggagggggcg gttgggaaag
    10141 gggggaggac aggagggggg cggcccccgc ccaggaaacg ccgccgaccg gccgaccgct
    10201 ggcctctcct cgccgtcagc cggccggggt ttcccggtca tcggcatggc ggcgcctgtc
    10261 attggcgcat cggcgcctgt cctcggccgg gtcaggcctg tcatcggcct ggctgtgccc
    10321 gctgtcggcc tggcggtgcc ggtcatcggc ctgggcaggc cggtcatcgg cgcggccagg
    10381 cccgtcatcg gcctggccgt gccggtcgcc ggcgaggctc tgccgacggc aggctgaggg
    10441 ctgcccgagc ctgggaacgt tctccctcct cctggacagt gatacttttc tcctcgcagg
    10501 cacgactctc ttcgagccct ggagggggcg gcgatcccat ggacttcgac actcggaaga
    10561 agtactacaa cctctgcaag ccgactgagc ctctggctcc tggcgatccg cgcaacctgc
    10621 ccatcgacga gctcgacgga gcggtacgag gctggcactg ggtggaccgc ttcgccaaga
    10681 agatcgggat ggtggaggag ccggtgctcg agctgttcac cggcctgccg ggctcgggca
    10741 agtccacgga gctgcggcgc ctctccgcgc ggctgtccga caagaagggc gcccatctcc
    10801 tgcccgtcct catcgacgcc gaggaggtca tcgacctcac caaccccatc gacgtcccgg
    10861 atatcctcgc agcgatcctc cacgagacgg agaagtgtgt ccttatccac gaggggcgca
    10921 attccgacca ggccctccag gagggctacc tcacgcggtt ctggcactgg ctgacgacta
    10981 cggacgtggc gatcgggcag gcgggatttt ccgtaccggg gggcggcccc aacctggtgg
    11041 cggagatgaa gacccgcccc tccctgcgca agagggtgcg ggagacggtg gcagcccacc
    11101 tttccggctt cctgggcgaa gtcgaggacg agatgagcct cctcgatcgc cgggcgcggg
    11161 acctcggcta ccgtggactg gtcgtcatct tcgactccct ggagaaactg cggggcatta
    11221 gcaccaattg gaaggatgtc ctcgacagcg ccgaggagat cttcgccgcc aacgcccccc
    11281 atctccggct gccggtccac gtcatctaca cggttcccgc cgccctggtc gcccgtgccc
    11341 ggatcgccaa cgtcgagttc atgccgatga tcaagctcca gacccgggaa ggcgagccgt
    11401 tcgagcccgg tatggaggcg gcccgcgagc tgatccgccg gcggattccg gacgacgtgc
    11461 tgggggagct gttcgggccg gatgccgagg tgcggctcgc gaagctgatc cgctggtccg
    11521 gcggctatcc tcgggagatc gtccggctgc tccgggaggt gctcctcctg tccgaggagc
    11581 cggttccaga cgaggatgtg gagcggattc tgggagaggt ccgaagctcc taccggatga
    11641 tggtctcctc ggacgccttc gaatggctgg cccgggtgtc ggtggaacgc ttcctgacgc
    11701 tggagaacga cgaccaccgc caggtggcgg accgtatgct gcagaacaac gcggtcctcc
    11761 gctatctcaa ccaggaatcc tggttcgacc tccaccccgc gctgtacgag atccccgggc
    11821 ttcagaaggt catcgcgcgg atgaagtccg gtggctgacc ccggcctgga taccctcacc
    11881 gccgcgctcc gggcagattg gggccttaac cgcctcttcc gcagtctggc cctcgccgag
    11941 ggttttcagt tccacatcct cgtctgcgcc agcccccggg tggcgcaggc ggccttccgg
    12001 ctcattgagg aggagacggg ctcggccgtt caccggctgc ggcccgagcc cttctatttg
    12061 agggagccgg tcccctacga gcatctgatc tcggcggttc tggagccgct ggtctggccg
    12121 caattcgaag gtgaggacgt gccccgggtg atcgtcatcg acgcttcgga tgccgctaag
    12181 agagatgaag ggtcctggcg ccagcttttc aggaggatga acgaacgacg caacgtgatc
    12241 gcgcaacggg tgccggggac gctcctcctc tgcgtgacgc cgagcctgca cctcgccttc
    12301 atgcaggagg ccccggattt ctggtccatc cggggggccg aggtgacgct ggagccgccg
    12361 ccggctccag cggagcctct cggagccagg ccggaggttg agcacgagcg acgcgaagaa
    12421 cgggcacctt cggtcgagac cgagccggag cggctgcggg ccgccgtcgc agtggcgcgc
    12481 aagcggatgg aggaactgcc ggaggacgcg tcggcgttgc gggcattgat gattcaactc
    12541 gaccgccttg gacgctatga ggtggagtgg gggagcctgg agagggctca gaccgcctat
    12601 gaggaggccg tagatctcgc gaggtggcgg atggagttgg cgccagaccg tccggagtgg
    12661 ctgcgggacc tatctgtgag cctcaatgac ctgggtgacg tgctggtgtc tcgtggctct
    12721 ctggagaggg cgcaggagtc gtacgaggag tctctgggtg tgatgcgtcg tcttctggag
    12781 caggacccgg accgtccgga gtggctgcgg gacctgtcgg tgagcctcat caaagtgggt
    12841 gacattctcc ggtctcgcgg ttttctggag agggcgcagg agtcgtacga ggagtctctg
    12901 agtgtgatgc gtcgtcttct ggagcaggat ccggaccgtc cggagtggct gcgggacctg
    12961 tcggtgagcc agggcaacgt gggtgacgtg cttcggtctc gtgggtctct ggagagcgcg
    13021 cagtcgtcgt acgaggagtc tctgggtgtg acgcgtcgtc ttctggagca ggacccggac
    13081 cgaccggagt ggctgcggga cctgtcggtg agcctcaaca aggtgggtaa cgtgcttctg
    13141 gctcgtggct ctctggagag ggcgcagtcg tcgtacgagg agtctctggg ggtgatgcgt
    13201 tgtcttctgg agcaggaccc ggaccgtccg gagtggctac gggacctttc ggtgagcctg
    13261 gagcgagtgg gtgacgtgct tcggtctcgt ggttctttgg agagggcgca gtcgtcgtac
    13321 gaggagtctc tgggtgtaag acgtcgtctt ctggagcagg acccggaccg tccggagtgg
    13381 ctgcgggact tggcggtgag ccttctaaga attgggacga ttcacgaggc caggggcgag
    13441 cgcgaacagg cgatcttgct gtatgagcag gctctacaaa tcgtcaggga tctccaggct
    13501 cgctttccat accagcctct ctatggtgaa gacgtggctt ggctggagag gcggttgaaa
    13561 gggctgcggg gagaaaaacc cccctcctga cctcccccgc tccctaccgc ccaccctccc
    13621 agggggaggc accaccgccc attaacgcct ctgttgcacg cgcggggcgc cgcttctaca
    13681 atcccgaggt actttccgcg acatccttcg gggctccggg aggtcgccca tccccgccgt
    13741 tttcttcttg acttccgttc tgtcttcccg gtaggctttc ggcataaccc gtgcggcagc
    13801 ggcccgaggt gagttcggac ctcaccccga gcctagccgc acacagaccg tgaggaggtc
    13861 ttatgagcag ccatgaagat ggtaacaccc cccgtgtacc caactcgccg gattccttga
    13921 acccgcaacc ggcccccgaa gctccggctt ctccttccaa cgcgttcaca aactcgctcc
    13981 tccagcgtct tcaggacagg gacgagccgc cggccgccga cgaggcggat tgggcggggc
    14041 cctggcggat ggagtccctc ccggatggca gcttcggctt cttccggagg ggggagagcc
    14101 ccgagcgggg gtaccggccc gccatgcggt tcgtccggcg gtccgacgcg ctcctcgcgc
    14161 tggcgatgat cgaggcccgg cgcgagcccg agtatcgcct cctgccggcg ccggacggcc
    14221 acggctatct cgtgcagagc cgctccgcgt ggggcgaggt cgtcgggggg ctcgtgatct
    14281 tcgatgacga cctcatcgcc cgcgtctcct gggcggaggc gctcatgcgg tcgcccgagg
    14341 cgctcgcgct cttcttggag gcgtgcggca aggtggtgct ggagcgcgcc ggcgccattc
    14401 tggaggagcg ggtgcgggcg atggctccct gacgtacatg cgtggccccg ggcctcgtcc
    14461 cggggccacg ccgtttccgc tatccttccc gcgccatgga actgctctac acctcccacg
    14521 tccccgcggg cgacggcccg catcccacgg tcatcctgct ccacggctgg ggcgccaacg
    14581 cccacgacct gctggggctc gcgccgatgc tccacggcgg gcgcgccctg gtgctctgcc
    14641 cccagggcca ggtggagttc tccatcggcg gcggcatggc gggctatgga tggttcccgc
    14701 tggtccccgg agcgccgccc gatccccagg cgttcctgcg ggcggccgac tcgctgcgcg
    14761 gcttcgtcga caaggcgctg gagcgctatc ccatcgatcc gaagcggctg gtggtggcgg
    14821 ggttcagcca ggggggtctc atggcctatg agctggggct gcgccagccg gcccgcttcg
    14881 cgggcatcgc cgcgctctcc tcctggttcc cccagccgct ggccgagaac ctgccccatc
    14941 tgcccgagca ccaggacttc cccgtgctga tggtccacgg cacccaggac accatgctgg
    15001 aggtggaccg cgcccgcgag gggcgggagc tgctgcgccc cttcggcgtc tccatcacct
    15061 accgggagtt cgacatgggc cacgagatcc ggcccgacgc cctgaaggtg atcctgcgct
    15121 ggctggacga gaaggcgttc gggcggacgg agggggctgc gggaggctag gcctcgcttc
    15181 tggcttcggt gagccccccg tcgaagtcgt cgtcgtcttc ctcatcgtcc aggggggctg
    15241 agaagagggt ccggagcacc gggtccgcgg tggcgcgcag ggcttcgagg acgcgcgcag
    15301 cggtggggat ctcctgctcc ggcaggtcgt cgatgagccg atggagtgct tctctgcgag
    15361 tcacattcac ctccgctcca tcaatctacc atcgcgggtc gagcttcggg cggacggagg
    15421 agcccttgcc cgccgctcag ccttctcgtt agtctttaga catgcccgat cagcagccgt
    15481 ccgtcgacgc cgcgaacgtc ttccaggtcc acttcctgga catggggtcg aagaagtacg
    15541 gggactgcat cgtctgcctc atcggggaca agcgaatcct gatcgatggc gggcacctcg
    15601 gcgaccagcg gcgcggccat ccggactatc cgtcgatccc ggagcagctc gcggagatcc
    15661 tggggcagcc cgccccgtgg cgtttcgacc tcctggtggt cacccacgcg cacgtggacc
    15721 acatcggctg cctgcccaac ctggtctcca cgggccagat cgtcgccgac tgggtgctgg
    15781 ccgccgacga ggagctgggc tggggggatg tcgaaggtgt ggacgccccg gccttcgagg
    15841 acgcgcccga gccggtgcgg caggcgttcg ccctgctccg ggaggagcgc cccgccttcg
    15901 ccagccgtga ggagatggag gccttcgcca gcgacgccgc gcgcatgcgg ccgacctatg
    15961 ccgccatgct ggacgccctc gacaggggtg gggccaaggt cgtccgcttc ggccgggatg
    16021 atcccgccga tctggtggcg gcgttgcggc cggagacggg cctggagatc ctggggccga
    16081 ctccggagca cctgctggcc tgtgcccgcc agatcgccgc gctgggccgt gatgccgcgg
    16141 acgtgatcgt cgagcggtcc aggcgggcgg atcacccgct ggacctggtc gaggcctacg
    16201 aggcgctgct gatgggctcg ttgtataggg acagcggcga gttcgagctg gggaacgcca
    16261 tcaacgacca gagcctcgtc ctcgccttcg ggcgcggcgg ccggaaggtg ctgctgacgg
    16321 gcgacatgca gttggccgac ccgctggtcg acggcctcga ggagagcatg gccgagctgc
    16381 gccggcacat cgcccgcaac ggcccgtact cgctggtgaa gctccctcac cacggcgccc
    16441 gcaacggcgt ggacgcggag ctgctggccg agaccggcgc gcctctcttc gtcatctccg
    16501 gcggccaggg gagcgccaag cacccgcatc ccagcactct cgaggtcctg gagacggccc
    16561 aggggatcgc ctggtaccgc acggaccgca acggctggat cagcgtcgac ctggcgacgg
    16621 cggagatcga ggcggagtgg gtccgcggcg agccgaacga ccgcggccgg aacccggagg
    16681 cgcgcgactt tctcgacgag ccgggccccg cgctcccgcc gccggccgaa ccgcccgctc
    16741 caccctctcc agaattcacg cgctccgtgg tgaggacgcc cgacggcgag atcgtggagg
    16801 ttctcgcccg gatcccgcac cgccggacca aggtggtcat caccatcgag gtcgagccgt
    16861 cagaggagcc atcaggagag atcgcggccg ccggccggcg ggagagcccg gcggccggag
    16921 ccgggaggcc ggccgcggac cgtgttaccc cacggccggc cggcggccag ggggcggcgc
    16981 tccgcctcgg cggcggccgg gagctcccgc cgctcctctt cgccaccgac cccgacgcgc
    17041 tggcgaacaa catcggccgc gaggaggccc gcgccgtctg caaggcgctg gaggattcgg
    17101 ggcagaccgt ggtcttcgac ggcttccgca agatgagctc cg
//
LOCUS       DASPJI010000843         8290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_112936, whole genome shotgun sequence.
ACCESSION   DASPJI010000843 DASPJI010000000
VERSION     DASPJI010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112936"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="VEL74_23750"
     CDS             <1..1585
                     /locus_tag="VEL74_23750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65618.1"
                     /translation="PAASQVRRPGDARRARTFDYQVPEEGISSEFDDGFNLTVDAAGN
                     VYAIGWSFVDGTRRRDFLVLKYDTNGVLQWTRTFDYGDDDYATRVVVDATGNVYVSGE
                     SQTPGLPYQSTDNVTIKYSPSGTELWRRVYAEAPGRWDGDTQMVLAPLGGVYVAMMSQ
                     IPEGYNNTVVHYLADGTEAWRYRIPGSGNDWLWDFALDPAGDLIVGGHELGETTATNF
                     LTVKLATSRGTAPGPRLAALSILPTRVTGGESATGTVTLDGPAPAGGAVVTVESGSTA
                     SAWTPASVTVPAGATTASFPVSTSANVNQDNLVDFSASYGGVAKLASLTVAKPPSPPP
                     PSAVLSSLTVTPLTFPGGCKTATGKVTLTAPAPAGGAWVPITNQHPVATMPSGVTVPA
                     GATSATFTISGPAVSSTVTGTVSASVGGVTKSVSVKVRPIAILSLTLSPNRLRGPGSS
                     TATVTLECPAAPGSISVVLSSSNTAVATPAVASLTIPAGARTATFQVNAADVSAVSTA
                     SISARGNGVTKTATITVEP"
     gene            complement(1645..3642)
                     /locus_tag="VEL74_23755"
     CDS             complement(1645..3642)
                     /locus_tag="VEL74_23755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG65619.1"
                     /translation="MATQTSTGQLDWPRATVESLGDAVVTTDSHGAVTFLNPAAVRLL
                     GRSAEEAGGSPLGQIVQLLDERTHRPLELPLNGAAAGQSSTRRALLAAASGADERVLD
                     VDVSPLQGEAGGAVLVLRDATERVRAERALRESEDRYRIFVSHSSEGIWRCDVEEPVP
                     TTLSEDQQIDLFYRHGVLAECNEVMARMYGVASAEEMRGARLDDLVPRSDSHNVEYLR
                     TFIRSGYHLTGAESHEVDKEGNPKYFINNLIGILEGDRLTGVWGTQLDITERKQLEEE
                     LRRRAEELVAADRRKDQFLAMLAHELRNPLAPIRNSVELMRQAENFDPSFQASREMVE
                     RQVKHLARLVDDLLDVSRITHGNIRLRKEGLDLGTILQRAVEANRSLIEARNHRLELR
                     LPAEPVRLEADPTRLEQIFSNLLNNAAKYTMPGGQIWLTAALEEGEAVVRLRDTGIGV
                     PPDVLGRVFEPFVQSDGSLARSEGGLGIGLTLARSLVEMHGGTVEAHSAGLGQGSEFV
                     VRLPARAAIPSEMPGLVPEPQPAAPSRGVRVLVVEDNVDAAESLAALLRLWNHDVQVV
                     HDGLEAVEAAREQRPEVVLLDIGLPGLDGYQVAQRLREDVGLDHALLVAMTGYGQPED
                     RRRSREAGIHHHFVKPVEPEVLRNLLAAQAAPLGTEPRPAF"
     gene            3754..5898
                     /locus_tag="VEL74_23760"
     CDS             3754..5898
                     /locus_tag="VEL74_23760"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HYG65620.1"
                     /translation="MKPAHRAWIPLILIVAAASHAEDNRSVGPLFPVPDAQRQAAPAA
                     PPPRLSETWGLVPLAVLAPAEKAARAELAALQSWNDAGRLPVRNGFTRALPAARAVTI
                     DAGALPRTPIEHAGGILAETAFGRLAWGGEVRVPGAYRLRARLTDVRLPRGTRLWVHG
                     DHGEVVGPFGPERRGPSGDLWTPSVGGESIRLDVELPAGAFEKAAEARFGFRIAEVLE
                     MVPMEDEMAGDKVLPCEVDLACADRSELPFLDAYRHAVARMTFVQDGSGFLCSGALLN
                     DTDSRTEIPYLLTAHHCLSTQEAASSLDAFWDYYRTSCGGPAPSLSQAPRSDGATLLA
                     TSNDTDFTLLRLDRVPPGRVFLGWTADASVIREDGTRLYRLSSPGGRPLAYSVTRVDR
                     FFQACRDRIRPDFIYSTRVTGGTEGGSSGAPTILADGRVVGQLLGGCGDSDDCSIGQA
                     TVDGAFSGTFPRVRQFLAPLQPCTPGPNNLCLLGNRFRVELVWQNQYDGSSGQGKPVP
                     RTDSTGFFYFQDPSNFELIVKILDFGDVVKVFYGQLTDLRFTLTVTDTRTGNTKIYQN
                     GPGDCGAIDQTAFPGSLASTSATSAVFEAARRGRAAKAGTCVADADTLCLVDRRFAVE
                     VNWRNQFNNESGRAQPAPLSQQAGMFSYTDRSNIELVVKALDFGDRILFFWGALSDLE
                     YTITLTDTETGASKTYNNPAGKFCGGLDNNAF"
     gene            complement(5844..7412)
                     /locus_tag="VEL74_23765"
     CDS             complement(5844..7412)
                     /locus_tag="VEL74_23765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65621.1"
                     /translation="MAANLLPALGGVALFLAAGFALTELFPALRRLSLPHRLAYAYLL
                     GVATVAGGLYTLSHLLDIPLRRPAIFGLALAVAAAGLLIRLLRAGGRRPPGVRSSGAG
                     DWQRGRVGGGSAHEGAEQGDRTPGGRRAGLRRLLPLVFPAVTLAVTLGVLADAVSEPV
                     RDWDGRMTWSAQARYLRADGTVNASALQRARWYISHPEYPLLLPVAQVAALEATGAGD
                     DDPTFRALYAAFLPALLLLIHDAARRWAGRAAADLAGLAAAAVPFFAYGGEGGAVSAY
                     SDLPLACFYGGGLILLLRSRLRPFDGIAAGLLLAAAVLTKNEGTPLTLLALMAAGLVI
                     VRAWLAARRRGAVPLSRTAHRRAARLIATALVAALALALLIDWRTGIPNREDENYAEF
                     VDLSDFWPGVVTRIPSVGSVMLRQMTAWEHWGVFWWALPLVLLAGWKGWTGRRRAVSL
                     PLLLALAGPLAVAWGAYSVHWYPEDLARVTWNRLLLQGAVPLFVLLALALRRLLPQKA
                     LLSKPPQNLPAGLL"
     gene            complement(7402..7866)
                     /locus_tag="VEL74_23770"
     CDS             complement(7402..7866)
                     /locus_tag="VEL74_23770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65622.1"
                     /translation="MRFRLSVPQAVALAAFGLFLATSLPVAMGTSANRVLQALRRAGE
                     SPDAARVRVFGAPYVETIDTIRRTIPPDGVYALVDGEPVEEGAALWVRHDLAPRKAVF
                     LGKTGKLPGVKRLRSRVPARARWVVIAYTNGRPPDMIERFRFLEELEARRGR"
     gene            complement(7889..>8290)
                     /locus_tag="VEL74_23775"
     CDS             complement(7889..>8290)
                     /locus_tag="VEL74_23775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65623.1"
                     /translation="AVTARTARASERRAIPAGALWIPADQPDFEVAVQLLEPEAPDSL
                     LAWGLLSTVFERKEYIEPRVLEGLAEEMLKDPKTAAAWQEALRDETFAKDGQARYMWW
                     YRRTPYWDETLGLLPVYRVMKAPALGTRGWK"
BASE COUNT         1209 a         2938 c         2995 g         1148 t
ORIGIN      
        1 acctgctgct tctcaagtac gacgcccagg ggacgctcgc cgggcgcgga ccttcgatta
       61 ccaggttccc gaggagggca tttcatcgga gttcgatgat ggcttcaatc tgacggtcga
      121 cgccgccggc aacgtctacg cgatcggctg gagcttcgtc gacgggaccc gccggcggga
      181 cttcctcgtc ctcaagtacg acacgaacgg tgtgctgcag tggactcgga ccttcgatta
      241 tggcgacgac gactatgcga cgcgcgtcgt ggtcgacgcc accggaaacg tctacgtcag
      301 cggcgaatcg cagacgcccg gtctcccgta ccaatcgacc gacaacgtga cgatcaaata
      361 cagcccgtcc gggaccgagt tgtggcggcg cgtctacgcg gaggcgccgg ggcggtggga
      421 cggcgacacg cagatggtgc tggcgccact gggtggcgtc tatgtcgcga tgatgtccca
      481 gatcccggag ggctacaaca acaccgtcgt gcactatctg gctgacggca ccgaggcctg
      541 gaggtaccgc atccccggca gcggcaacga ttggctctgg gatttcgcgc tcgatccggc
      601 cggggatctc atcgtcggcg gccacgagtt gggcgagacg acggcgacga acttcctcac
      661 cgtcaaactg gcgacctcgc ggggtacggc acccggtccg cggctcgccg cgttgtcgat
      721 cttgcccact cgggttacgg gcggcgagtc ggcgaccgga acggtgaccc tcgacgggcc
      781 ggcgcccgcc ggtggtgccg tggtgacggt agagagcgga tcgaccgcct cggcgtggac
      841 tccggcgagc gtcaccgtgc ccgcgggcgc gacaaccgcg tccttcccgg tcagcaccag
      901 cgccaatgtc aaccaggaca acctcgtcga tttctccgct agctacggcg gggtagcgaa
      961 gctcgcgtcg ctcaccgtcg cgaagccccc gtcgccaccg ccgccctcgg cggtgctgtc
     1021 gtccttgacc gtcactcctc tcactttccc gggcggttgc aagaccgcga ccggcaaggt
     1081 gacgctcacc gcgccggcgc ccgccggcgg cgcctgggtg ccgatcacca accagcaccc
     1141 ggtggcgacg atgccgagcg gggtgacggt gcccgctggc gcgacgagcg cgaccttcac
     1201 catctcgggc ccggccgtca gctcgacggt gaccggcacg gtgagcgcga gcgtgggtgg
     1261 cgtgacgaag tcggtcagcg tcaaggtgcg gccgatcgcg atcctgagcc tgacgctgtc
     1321 gcccaaccgg ttacgcggtc cgggctcgtc gaccgccacc gtgacgctcg agtgcccggc
     1381 ggcgccgggc tcgatctcgg tcgtgctgtc gtcgagcaac accgccgtgg caacgccggc
     1441 ggtcgccagc ctgaccatcc ccgccggcgc caggacggcg accttccagg tgaacgccgc
     1501 cgacgtctcg gcggtgagca ccgcctctat ctcggcccgt ggcaatgggg tgaccaagac
     1561 cgccacgatc accgtcgagc cataggacac cctcgccgcc gccgggaggt ccggcggcgg
     1621 cgtttccttt tgaatggggg aggctcagaa ggccggccgc ggctcggtcc ccagcggggc
     1681 ggcctgggcg gcgaggaggt tgcgcagcac ctcgggctcc acgggcttga cgaagtggtg
     1741 gtggatgccc gcctcgcgcg agcggcggcg gtcctccggt tgtccgtagc cggtcatcgc
     1801 caccagtagg gcgtggtcca ggcccacgtc ctcgcgcagg cgctgggcca cctgatagcc
     1861 gtccagcccc ggcaggccga tgtcgagcag cacgacctcg gggcgctgct cccgggcggc
     1921 ctccaccgct tccaggccgt cgtgcaccac ctgcacgtcg tggttccaga gacggagcag
     1981 cgcggcgagg ctctccgccg cgtcgacgtt gtcttccacc accagcaccc gcaccccccg
     2041 cgaaggggcc gccggctggg gctcggggac cagcccgggc atctccgacg ggatggcggc
     2101 gcgggcgggc aggcgcacca cgaactcgct cccctggccc aggccggcgc tgtgagcctc
     2161 caccgtgccg ccgtgcatct ccacgaggct gcgcgccagc gtcaggccga tgccgaggcc
     2221 cccctccgag cgggcgaggg agccgtccga ctgcacgaag ggctcgaaga cccggccgag
     2281 cacgtccggc ggcacgccga tccctgtgtc gcggaggcgt accacggcct caccctcctc
     2341 gagggcggcg gtgagccaga tctggccgcc cggcatcgtg tacttggccg cgttgttgag
     2401 gaggttcgag aagatctgct ccagacgggt gggatcggcc tccagccgca ccggctcggc
     2461 ggggagccgc agctccagcc gatgattgcg cgcctcgatc agcgaccggt tggcctccac
     2521 cgcccgctgc aggatcgtgc ccaggtccag cccctccttg cgcagccgga tgttgccgtg
     2581 cgtgatgcgt gagacgtcca gcaggtcgtc caccagccgg gcgaggtgct tgacctggcg
     2641 ctccaccatc tcgcgcgagg cctggaagga gggatcgaag ttctccgcct gccgcatcag
     2701 ctccaccgag ttgcggatcg gcgccagcgg attgcgcagc tcgtgcgcca gcatggcgag
     2761 gaactggtcc ttgcggcggt ccgcggctac cagctcctcg gcgcgccggc gcagctcctc
     2821 ctccaactgc ttgcgctccg tgatgtccaa ctgcgtgccc cagacgccag tcaggcggtc
     2881 gccttccagg atcccgatga ggttgttgat gaagtatttc gggttgcctt ccttgtcgac
     2941 ctcgtgggac tccgccccgg tcaggtggta gccggagcgg atgaaggtgc gcaggtactc
     3001 gacgttgtgg gagtccgagc ggggcaccag atcgtcgagg cgcgcgccgc gcatctcctc
     3061 ggcgctcgcc acgccgtaca tgcgagccat gacctcgttg cactcggcga gcacgccgtg
     3121 gcgatagaag aggtcgatct gctggtcctc ggagagggtg gtgggcaccg gttcctcgac
     3181 gtcgcaacgc cagatcccct ccgagctgtg ggagacgaag atccggtacc ggtcctcgct
     3241 ctcccgcagc gcccgctccg cccgcacgcg ctcggtcgcg tcccgcagca ccagcaccgc
     3301 tcctccggcc tcgccttgga ggggagagac gtcgacatcc agcacgcgct cgtccgcgcc
     3361 gctcgcggcc gccagcaagg cgcggcgggt ggaggactgg ccggcggccg ctccattcag
     3421 cggcagctcc agggggcggt gggtccgctc gtccagaagc tggacgatct gtccgagagg
     3481 agagccgccg gcctcctcgg cgctccggcc gaggagccgc actgcggcag ggttcaggaa
     3541 ggtcacggca ccgtggctgt cggtcgtcac aaccgcgtcg ccgaggcttt ccacggtggc
     3601 gcggggccaa tcgagctggc cggtggaggt ctgcgttgcc atagatcctt tctaatagcc
     3661 ggcgacggtc ctccccgtcg ggagggtcgt atcttaaacc ttccggcgtc cacacctata
     3721 cttgggggca ccgatgccga ctggagacgc tgcatgaagc ctgcccaccg agcctggatc
     3781 cccctcatcc tgatagttgc cgccgcctcc catgccgagg acaaccgaag tgtcgggccg
     3841 ctcttcccgg tccccgatgc ccagcgccag gccgcccctg cggctccgcc cccgcggctg
     3901 tccgagacct ggggcctcgt cccgctggcc gtcctggcgc cggccgagaa ggcggcgcgg
     3961 gccgagctcg cggcgctcca gtcctggaac gacgccggcc ggctgccggt gcggaacggc
     4021 ttcacccgcg ccctgcccgc cgcccgtgcc gtgacgatcg acgccggcgc gctgccgcgg
     4081 acgccgatcg agcacgccgg gggcatcctg gccgagaccg ccttcggccg gctggcctgg
     4141 gggggcgagg tgcgggttcc gggcgcctac cggctgcggg cgcggctcac cgacgtgcgg
     4201 ctcccccgcg ggacccgcct ctgggtccat ggggatcacg gcgaggtggt ggggcccttc
     4261 ggccccgagc ggcggggacc ctccggcgat ctctggactc ccagcgtcgg cggcgagtcg
     4321 atccggctgg acgtggagct gcccgccggc gcgttcgaga aggcggcgga ggcacgattc
     4381 ggcttccgga tcgccgaggt cctggagatg gtgccgatgg aggacgagat ggcgggcgac
     4441 aaggtgctgc cctgcgaggt cgacctcgcc tgcgccgacc ggagcgagct gcccttcctc
     4501 gacgcctacc gtcacgcggt ggcgcgaatg acgttcgtcc aggacggcag cggcttcctc
     4561 tgctccggcg cgctgctcaa cgacaccgat tcgcggaccg agatcccgta cctgctgacc
     4621 gcccaccact gcctctcgac ccaggaggcg gcgtcgagcc tggacgcctt ctgggattac
     4681 taccggacct cctgcggcgg cccggccccg tcgctgagtc aggccccgcg cagcgacggc
     4741 gcaaccctcc tcgccacctc caacgacacc gacttcaccc tgctgcgcct ggaccgggtc
     4801 ccgccggggc gcgtcttcct cggctggacc gccgatgcgt cggtcatccg ggaggacggc
     4861 actcgcctgt atcgcctgtc cagtcccggc ggccgtcccc tggcctactc ggtgacccgg
     4921 gtggaccgct tcttccaggc ctgccgcgac cggatacgcc cggacttcat ctattcgacc
     4981 cgggtcaccg gggggaccga gggcggcagc tccggcgcgc cgaccatcct ggccgacggc
     5041 cgcgtcgtcg gccagctcct cggcggctgc ggcgacagcg acgactgcag catcggacag
     5101 gccaccgtcg acggcgcgtt ctccggcacc ttccccaggg tccgccagtt cctggccccc
     5161 ttgcagccct gcacgccggg gccgaacaac ctctgtctcc tggggaaccg tttccgggtg
     5221 gagctggtct ggcagaatca gtacgacggc tcgtcgggcc aggggaagcc ggtgccccgc
     5281 accgactcca cgggcttctt ctacttccag gacccgtcca acttcgagct gatcgtcaag
     5341 atcctggact tcggcgacgt ggtcaaggtg ttctacggcc agctcaccga cctgcgcttc
     5401 accctgaccg tcaccgacac ccggacgggc aacaccaaga tctaccagaa cggccccggc
     5461 gactgcggcg ccatcgacca gacggccttc ccgggctcgc tggcgagcac ttcggcgaca
     5521 tcggcggtgt tcgaggcggc gcggcggggg agagcggcca aggccggcac ctgcgtggcc
     5581 gatgccgaca ccctgtgcct ggtcgaccgc cgcttcgccg tcgaggtcaa ctggcgcaat
     5641 cagttcaaca acgaatccgg cagggcccag ccggcgccgc tcagccagca ggccggcatg
     5701 ttctcctaca ccgaccggag caacatcgag ctggtggtca aggccctgga cttcggcgac
     5761 cgcatcctct tcttctgggg cgcgctgtcg gacctggagt acacgatcac cctgacggac
     5821 acggagaccg gagccagcaa gacctacaac aacccggccg gcaagttctg cggcggcttg
     5881 gacaacaacg ccttctgagg caggaggcgc cgcagggcca gggccaggag gacgaagagc
     5941 ggcacggcgc cctggagcag cagccggttc caggtgaccc gggcgaggtc ctccgggtac
     6001 cagtgcacgg agtaggcccc ccaggcgacg gccagggggc cggccagggc caggagcagg
     6061 ggcagcgaga ccgcccgcct ccggcccgtc catcctttcc atcccgccag caggaccagg
     6121 gggagcgccc accagaagac tccccagtgc tcccaggctg tcatctgccg gagcatcacc
     6181 gatccaacgg aggggatgcg ggtgaccacc ccgggccaga agtccgagag gtcgacgaac
     6241 tcggcgtagt tctcgtcctc gcggttgggg attcccgtcc gccagtcgat gaggagcgcc
     6301 agggccaggg cggccaccag ggccgtggcg atgagccggg ccgcccggcg gtgggcggtc
     6361 cgggagaggg ggaccgcgcc acggcggcgg gcggccagcc aggcgcggac gatgaccagc
     6421 cccgccgcca tgagggcgag gagcgtcaac ggggtgccct cgttcttggt caggacggcg
     6481 gcggcgagga ggaggcccgc ggcgatgccg tcgaagggcc ggagccggga gcgcaggagg
     6541 aggatgaggc cgccgccgta gaagcaggcg agagggaggt cgctgtaagc gctgaccgct
     6601 cccccctcgc cgccgtaggc gaagaacggc acggcggcgg cggccaggcc ggcgaggtcg
     6661 gcggcggcgc ggccggccca gcgccgggcg gcgtcgtgga tgaggagcag gagggcgggt
     6721 aggaaggccg cgtagagcgc ccggaaggtg gggtcgtcgt cacccgcgcc ggtagcctcc
     6781 agcgccgcca cctgggcgac ggggaggagg agcgggtact cggggtggga gatgtaccag
     6841 cgggcccgct ggagagcgga ggcgttcacg gtgccgtccg cgcggaggta gcgggcctgg
     6901 gccgaccagg tcatccggcc gtcccagtcg cggaccggct cgctcacggc gtccgccagg
     6961 acgccgaggg tgacggcgag ggtgacggcg gggaagacga gggggaggag gcggcggaga
     7021 cccgcccgac ggccgccggg ggtgcggtcg ccctgctcag ctccttcgtg tgcgctcccc
     7081 cctcccaccc tcccacgctg ccagtcgcct gcgccggacg accgcacccc cggcggcctc
     7141 ctcccacccg ctcgtaggag gcggatgagg aggcccgcgg cggctacggc gagcgccagg
     7201 ccgaagatgg cggggcggcg gagggggatg tcgaggaggt gggagagggt gtagaggcct
     7261 ccggcgacgg tggcgacgcc tagaagatag gcataggcga gccggtgggg gagcgagagg
     7321 cggcggagag cgggaaagag ctcggtgagg gcgaagccgg cggccaggaa gagggcgacg
     7381 ccgccgaggg cgggcaggag gttagcggcc acggcgggcc tccagctctt cgaggaaacg
     7441 gaagcgctcg atcatgtccg gcggccggcc gttggtgtag gcgatgacga cccagcgggc
     7501 ccgcgccggc acccggctgc gcaggcgctt cactccgggg agcttgcccg tcttgccgag
     7561 gaagacggcc ttgcggggag ccaggtcgtg gcgcacccag agcgccgccc cctcctcgac
     7621 cggctcgccg tcgaccagcg cgtagacccc gtccggcgga atggtgcggc ggatggtgtc
     7681 gatggtctcg acgtaggggg cgccgaagac gcgcacccga gccgcgtccg ggctctcgcc
     7741 ggcacggcgg agcgcctgga ggacacggtt ggccgacgtc cccatggcca cggggagcga
     7801 ggtggccagg aacaggccga acgcggccag ggccacggcc tggggaacgg agagccggaa
     7861 gcgcatcggg cggctattga atcacagcct acttccaccc ccgcgtcccg agcgccggcg
     7921 ccttcatcac ccggtagacg gggaggagcc ccagggtctc gtcccagtag ggggtccggc
     7981 gataccacca catgtagcgc gcctggccgt ccttggcgaa ggtctcgtcg cggagcgcct
     8041 cctgccaggc ggccgcggtc ttcgggtcct tcagcatctc ctcggccagc ccctcgagga
     8101 cgcgcggctc gatgtactcc ttgcgctcga agacggtgga gaggagcccc caggcgagga
     8161 gcgagtccgg ggcctcgggc tccaggagct gcaccgccac ctcgaagtcg ggctggtccg
     8221 ccgggatcca cagggcgccc gcggggatgg cgcgccgctc ggacgcacgc gcggtcctgg
     8281 cggtcaccgc
//
LOCUS       DASPJI010000844         4407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_567415, whole genome shotgun sequence.
ACCESSION   DASPJI010000844 DASPJI010000000
VERSION     DASPJI010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567415"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..724)
                     /gene="folE"
                     /locus_tag="VEL74_23780"
     CDS             complement(89..724)
                     /gene="folE"
                     /locus_tag="VEL74_23780"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HYG65624.1"
                     /translation="MSRDPEVIVPEHVPPVALTELDGSQQQKLDRVAHHVRGLLTALG
                     LDLKDPNLAETDLRVAKMYLEMFHGLEEGAEPKVTTFPNEEKYSHMVMEKQIPFYSMC
                     AHHLVPFYGHAHIAYIPRDRILGLSKFARILEFYAKRPQLQERLTEQVVTFLEEKLQP
                     LGAMVVIEARHLCVEMRGVKKPGALTVTSALRGIFHQKPVREEFLDLLRHS"
     gene            866..1315
                     /locus_tag="VEL74_23785"
     CDS             866..1315
                     /locus_tag="VEL74_23785"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HYG65625.1"
                     /translation="MAKSARDIMSSDPACVTPVATAQEAAGLMETHDVGSLPVVESES
                     SKRLLGIVTDRDLALRVLGRGESGNTPVSEVMSKDLHCARQEDDLDKVERLMAEHQVR
                     RIPVLDEQDRLVGMIAQADLARERRAVGDKDFAKVVEQISEPAGVGR"
     gene            1433..2074
                     /gene="kynB"
                     /locus_tag="VEL74_23790"
     CDS             1433..2074
                     /gene="kynB"
                     /locus_tag="VEL74_23790"
                     /EC_number="3.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylformamidase"
                     /protein_id="HYG65626.1"
                     /translation="MLYDLSPTIRSGFPVWPGDTAFESRLIWSLAEGASVNLSAVTTT
                     PHLGSHADAPFHTEERGDFIADMPLERYIGPCRVVKVPPQPLIEPRHLEGIDLASPSR
                     ILFKSESVRDRRTFPERFTALSPEVAALLADRGILLVGMDTPSVDPFDSKTLDAHHAL
                     FRGGVAILEGLVLDGVPEGIYELIALPLKLAGLDASPVRAVLRTFAREEVASR"
     gene            2165..2824
                     /locus_tag="VEL74_23795"
     CDS             2165..2824
                     /locus_tag="VEL74_23795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HYG65627.1"
                     /translation="MNSKEFVGQLVEEMEGLFARLGETETLEAEADGQVDVVTLLRLA
                     LKSELEASELAAFWMASTPEMDAKMILAMQVGDEMKHYGMISQRLEDLGADLSAFDPL
                     AEGHSPLYHYLRGLRTTVERIAAGPFASEAIAKIRNAQFVHFCRSVGDEQTARLYEKV
                     IQPEEIHHYHAGREFLEKHATTPELQALAAAAARNTLEIADELRSLAAKTTGIHSIPV
                     S"
     gene            2862..3740
                     /locus_tag="VEL74_23800"
     CDS             2862..3740
                     /locus_tag="VEL74_23800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HYG65628.1"
                     /translation="MPPEISTLAVLGSGTMGRGIAHVAAVAGYTTHLFDPDAAALSKA
                     ESTIHRNLAKGVELGKVDAAVAERARGNLRLDTDLGAAVAEADLVIEAAPEDIALKVD
                     LFRQVARSVPEHALFASNTSALSITEMAAASGRPERFAGMHFFNPVHLMRLIELVRGL
                     DTSQETLDAMRRVGERMGKEIVLVNEAPGFVTSRINVLIGNEAFRMLQEGVASAEDID
                     KALKLGLNHPMGPFELADLVGLDVRLSILEFLHRSLGEAFRPNQLLIQHVRAGRLGRK
                     VGRGVYTYNENGERVK"
     gene            3737..>4407
                     /locus_tag="VEL74_23805"
     CDS             3737..>4407
                     /locus_tag="VEL74_23805"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HYG65629.1"
                     /translation="MTDLAGRVALVTGASSGLGLASARALARRGARLAIASRGGDKLE
                     GAREELARAGADVASFAADIREPEELERLVREVEQRLGGVDILIANGGGPPAKPALDL
                     TEEDWQAALPLVFLFIPRLCRLVVPGMRQRRWGRIVAINSVSARQPIPNLALSNALRP
                     AVLGYLKTLSQEVAADGVTVNAVLPGYILTARQEELTQAAAARSGRSAEEIVSARVAD
                     IPMGR"
BASE COUNT          681 a         1578 c         1523 g          625 t
ORIGIN      
        1 gccgccgccg gtgccgccgc tgccgcagcc ggccagcccc agggcggcgc ccagggtgag
       61 gctcagcagc agagcggagg cggcggatct acgaatgacg gagcaggtca aggaactcct
      121 ctcggaccgg cttctgatgg aagatcccgc gcaacgccga ggtgacggtc agcgcccccg
      181 gcttcttgac tccgcgcatc tcgacacaca ggtgccgggc ctcgatcacc accatggctc
      241 ccagcggctg gagcttctcc tccaggaagg tcaccacctg ctcggtcaga cgctcctgga
      301 gctgcggccg cttggcgtag aactccagga tgcgggcgaa cttcgacagc cccaggatgc
      361 ggtcccgggg gatgtaggcg atatgggcgt ggccgtagaa cgggaccagg tgatgagcgc
      421 acatggagta gaaggggatc tgcttctcca tcaccatgtg gctgtacttc tcctcgttcg
      481 ggaaggtggt gaccttcggc tcggccccct cctccagccc gtggaacatc tcgaggtaca
      541 tcttggcgac ccgcaggtcg gtctcggcca ggttggggtc cttgaggtcc aaccccaggg
      601 ccgtgagaag gccgcgcacg tggtgcgcga cccggtccag cttctgctgc tgcgagccgt
      661 cgagctcggt cagggcgacc ggcgggacgt gctcggggac gatcacttcg ggatcgcggc
      721 tcatggtctt gggctcctcg ccgggttcgg ccccgggcga atggcccact atcctacacc
      781 agcgcgcccc ccggcccgcc tcttgcagtc ccccctcttc gagagcgaag catcgctcgc
      841 cgttcgaact acaaggagga ctgtgatggc caagagtgca cgcgacatca tgagcagcga
      901 tcccgcctgc gtgaccccgg tcgcgaccgc ccaggaggcg gccggcctga tggagaccca
      961 tgacgtgggg agcctccccg tcgtcgagag cgagagctcg aagcgcctgc tcggcatcgt
     1021 caccgaccgc gacctggccc tgcgggtgct cggccggggc gagtccggca acaccccggt
     1081 gagcgaggtg atgtccaagg acctccactg cgcccgccag gaggacgacc tcgacaaggt
     1141 cgagcgcctg atggccgagc accaggtgcg ccgcatcccc gtgctcgacg agcaggaccg
     1201 tctggtcggc atgatcgccc aggccgacct cgcccgcgag cgccgcgccg tcggcgacaa
     1261 ggacttcgcc aaggtggtcg agcagatctc cgagccggcc ggcgtcggcc gctgacgggc
     1321 cgccagcttg gtccccggca gagggcgggg gagggaccgc ccgacggagg ccccctcccc
     1381 cggcatcatt cccctctgaa tcggggatat catccggggc caggagggct gcatgctcta
     1441 cgacctctcc cccacgatcc gttccggatt tcccgtctgg cccggcgaca ccgcgttcga
     1501 gagccggctc atctggtcgc tcgccgaggg cgcgtcggtc aacctgtcgg cggtcaccac
     1561 gacgccgcac ctgggatcgc acgccgacgc cccgttccat accgaggagc ggggggactt
     1621 catcgccgac atgccgctcg agcgctacat cggcccctgc cgggtggtga aggtgccgcc
     1681 gcagccgctg atcgagcccc gccacctgga ggggatcgac ctggcgagcc cgtcgcgcat
     1741 cctcttcaag agcgagagcg tgcgcgaccg ccggaccttc ccggaacggt tcaccgccct
     1801 gtcgcccgag gtggcggccc tcctcgccga ccgcggcatc ctcctcgtcg ggatggacac
     1861 gccctcggtg gacccgttcg acagcaagac cctggacgcg caccacgccc tgttccgcgg
     1921 cggcgtcgcc atcctggagg ggctggtcct cgacggcgtg ccggagggga tctacgagct
     1981 gatcgccctg ccgctcaagc tggcggggct cgacgcctcc cccgtccggg cggtgctgcg
     2041 taccttcgcg cgcgaagagg tcgcgtccag ataagatgag aggccggagg aggcgccggg
     2101 cccgttccgg gtccccgcga caacataccg gcaaccaggg ggcaaccccg aggagagatc
     2161 tctcgtgaac agcaaggaat tcgtcggcca gctggtcgag gagatggagg ggctcttcgc
     2221 ccgcctgggc gagaccgaga ccctggaagc cgaggccgac gggcaggtgg acgtcgtcac
     2281 cctgctccgc ctggccttga aaagcgagct cgaggcgagc gagctggccg ccttctggat
     2341 ggcctcgacg cccgagatgg acgccaagat gatcctcgcc atgcaggtcg gcgacgagat
     2401 gaagcactac ggcatgatca gccagcggct ggaggacctc ggcgcggacc tctccgcctt
     2461 cgacccgctg gcggaggggc acagccccct ctaccattac ctgcgcggcc tgcgcacgac
     2521 ggtggagcgc atcgccgccg gcccgttcgc cagcgaggcc atcgccaaga tacgcaacgc
     2581 ccagttcgtc catttctgcc ggtcggtggg ggacgagcag acggcgcggc tctatgagaa
     2641 ggtgatccag ccggaggaga tccaccacta ccacgccggc cgcgagttcc tggagaagca
     2701 cgcgacgacg ccggagctcc aggcgctggc cgccgccgcg gcccgcaaca cgctggagat
     2761 cgccgacgag ctgcgcagcc tggcggcgaa gacgaccggc atccacagca tccccgtttc
     2821 ctgaaccacc gacccgattc atcgactacg agaaaggacc catgcctccc gagatctcga
     2881 ccctcgccgt cctcggctcc ggcaccatgg ggcgcggcat cgcgcacgtg gccgccgtgg
     2941 ccggttacac cacccacctc ttcgacccgg acgcggcagc gctgtccaag gccgagtcca
     3001 ccatccaccg caacctcgcc aagggggtgg agctgggcaa ggtggatgcc gccgtcgccg
     3061 agcgggcgcg cggcaacctg cgtctggaca cggacctggg ggccgcggtg gccgaagccg
     3121 atctggtgat cgaggcggcg cccgaggaca tcgccctcaa ggtcgacctc ttccggcagg
     3181 tggcgcggtc cgtcccggag cacgccctgt tcgcctccaa cacctcggcc ctgtcgatca
     3241 ccgagatggc ggcggcctcg gggcgtccgg agcgcttcgc cggcatgcac ttcttcaacc
     3301 ccgtgcacct gatgcggctg atcgagctgg tgcgcggcct cgacacctcg caggagaccc
     3361 tcgacgccat gcgccgggtc ggcgagcgca tgggcaagga gatcgtgctg gtcaacgagg
     3421 cccccggctt cgtcaccagc cgcatcaacg tcctgatcgg caacgaggcc ttccgcatgc
     3481 tgcaggaggg ggtggcgagc gccgaggaca tcgacaaggc gctgaagctg ggcctgaacc
     3541 accccatggg ccccttcgag ctggcggacc tggtggggct cgacgtccgc ctgtcgatcc
     3601 tcgaattcct ccaccgctcc ctgggggagg ccttccggcc caaccagctc ctgatccagc
     3661 acgtccgcgc cggccgcctc ggccgcaagg tgggccgcgg cgtctacacc tacaacgaga
     3721 acggggaacg ggtgaagtga cggacctggc gggacgggtc gccctggtga ccggagcgag
     3781 cagcggcctg gggctggcct cggcccgggc gctcgcccgg cgcggcgccc gtctcgccat
     3841 cgccagccgc ggcggcgata agctggaggg agcccgggag gagctggcgc gcgcgggggc
     3901 cgacgtggcc tcgttcgccg cggatatccg cgagcccgaa gagctggagc ggctggtgcg
     3961 cgaggtggag cagcggctcg gcggcgtgga catcctgatc gccaacggcg gcggtccccc
     4021 ggccaagccg gcgctggacc tgacggagga ggactggcag gcggcgctgc cgctggtctt
     4081 cctcttcatc ccgcgcctct gccgcctggt cgtccccggc atgcggcagc gccgctgggg
     4141 ccggatcgtg gcgatcaact cggtctcggc gcggcagccg atcccgaacc tggcgctctc
     4201 gaacgctctg cgtccggcgg tgctcggcta tctcaagacc ctctcgcagg aggtggcggc
     4261 ggacggggtg acggtgaacg ccgtcctccc cggttacatc ctcaccgcgc ggcaggagga
     4321 gctgacccag gcggccgcgg cgcgctccgg ccgttccgcg gaggagatcg tcagcgcccg
     4381 cgtcgccgac atccccatgg gccgaat
//
LOCUS       DASPJI010000845         7740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_798142, whole genome shotgun sequence.
ACCESSION   DASPJI010000845 DASPJI010000000
VERSION     DASPJI010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798142"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VEL74_23810"
     CDS             complement(<1..423)
                     /locus_tag="VEL74_23810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG65630.1"
                     /translation="MRAVAFLLAGAVAALPSLPDIPWPSLHLPAAVERWLYNPRERTA
                     EAIEAAAQGEADRAADLADTALRIDEKDPAVRYNAGTAQLSAGHERKAVPLLEQAALE
                     AGPALSPAAHYNLGNAKLAAGDAAGAVEAYKAALRAEPG"
     gene            complement(420..1478)
                     /locus_tag="VEL74_23815"
     CDS             complement(420..1478)
                     /locus_tag="VEL74_23815"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG65631.1"
                     /translation="MSFAEPRLVWLALLAPLAALAVAWLWRRRLAADAAWASRGLWDR
                     LLPAYHPRRITLSVVCLGLAVLGAALALARPRWGSGEQTVERRGVDVVFLVDSSLSMG
                     VMDVSPSRLYVARTLVRRLAQAMPGNRLGLVQTEGAAMVLTPLTLDGAVVDLLLDTIE
                     PGSLPTPGTELSPGIETALRLFGEGSEKHRVLIVLSDGEDHGGRLDSWLPRLKEEGVT
                     VHAFGIGTPEGAPVPIPGSRDFKRDETGDLIVSRLHEETLEGLSRATGGAYLRVTSAA
                     ADPAPLLRQIDQMEKRTMESQSLSTHEERFQWPLGAAVAALLLYLAVGPFAPARETSL
                     SKGAAGEPSAEAARRKAA"
     gene            complement(1489..2502)
                     /locus_tag="VEL74_23820"
     CDS             complement(1489..2502)
                     /locus_tag="VEL74_23820"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG65632.1"
                     /translation="MMGEAFPHLASPWWLLLLLALPVLAWRHHRRQTLGALTYSHLPR
                     GAGRTAGAWRLHLPFYARLLALGLLVAALARPQLGYAWEESLTEGIDIQIVLDISGSM
                     GAEDFQPNNRLTVAKQVVTDFVQGRSGDRIGMTVFAGSALTRAPLTTDRQMLRQLISS
                     IELNTLPDGTAIGVALANAAARLKNSEAKTRVVLLVTDGVNNAGEIDPVSAAAVCDGL
                     GIKVYTIGVGAATGVAPVPFPQRDPVTGRQYVRRVMMRVPVDEELMRQIAKRTGGQFY
                     KATDGEGLRRIFDQIDRLEKTPLQVKRYVRYREAFAPLVWAALSLLLLPIAAAGLKVT
                     AEP"
     gene            complement(2499..3491)
                     /locus_tag="VEL74_23825"
     CDS             complement(2499..3491)
                     /locus_tag="VEL74_23825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65633.1"
                     /translation="MRALYPLLLMLLVGAPLVAQAPAPSTATEPRLTVTFSPPSQLTV
                     GDRVEATLDLTVRTADLTGEPRFPSWQEGWGEAEIVEKGAPEKVREEGGVATYRQRLV
                     LAAFRPGKVALPPMAVALPGARGTAQALTPPDLALEIGSVLPAEEKNPEARPPAALRA
                     LPLGERFWWTLAAASALCLGLLFLLWRQARRSAAAAPAAPPLPPFDELLRELDNLQRE
                     PSPLAVHTRLSLALRHYLGRALSFPAAESTTSEIQRQLVYRRVPGTPARRLVELLRAC
                     DLVKFARQDAGTEPTRERIGTAREIAREVEMYVQPPALQEKPPEQSPAKLEAAG"
     gene            complement(3488..4426)
                     /locus_tag="VEL74_23830"
     CDS             complement(3488..4426)
                     /locus_tag="VEL74_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HYG65634.1"
                     /translation="MFGLFSSRKKAPAERPNAPSSGQLPADLMRKVRRIEIATNRLVD
                     QGVAGDYHAVFKGRGMEFAEVRPYQPGDEIRTIDWNVTARMGSPFIKKYVEERDLTVF
                     LVVDVSGSLSFGSRAILKRELAAEIAALLAFAAVRNQDRVGAALVSDHLELFLPPQRR
                     RTHVLRLVREVLDRPAHGGTDLERGLHGVLAALRRRSVLFVISDFLGALPERALKAAA
                     ARHDLILVEIVDPRDQEIPSVGPVVLRDAETGRLALVNGRRLAKKHAEQRRQERAGLT
                     RLSRRIGVDHLELRTDRPYLGTVLSFFEQRRRRLRR"
     gene            complement(4446..5435)
                     /locus_tag="VEL74_23835"
     CDS             complement(4446..5435)
                     /locus_tag="VEL74_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYG65635.1"
                     /translation="MELDVPTLNRDVERETLTLKRIEAELRKVIVGQDYLLDRLLVGL
                     LARGHVLIEGVPGLAKTLAVKTLAQTLAIDAQRIQFTPDLLPADLIGTLIYDQRSGDF
                     LPRKGPIFTHLLLADEINRAPAKVQSALLESMEERQVTLGDRTWPLPEPFMVLATQNP
                     IEQEGTYPLAEAQVDRFMMKLRVGYPSRAEEAEILDRMLVENDLRPAPVLERDRLLAL
                     RAVVDSVYLDKKLRGYIIDLVTATRDPRSVGLGELSDLISFGASPRATLFLARAAKAL
                     AMVRGRGFVIPEDVKELAPDILRHRIIPTYEAEAEEISADDLVKRLLDRVPVP"
     gene            5609..6427
                     /gene="rsmA"
                     /locus_tag="VEL74_23840"
     CDS             5609..6427
                     /gene="rsmA"
                     /locus_tag="VEL74_23840"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HYG65636.1"
                     /translation="MSRPPLKKSLGQHHLIDGALTRPLVRFLAPLPTEAGRVLEIGPG
                     GGVLTAELLEAGARVVGWELDLQWAAFLGRRFRAAPLSLVVGDALEIAWGRLPVPTLV
                     AGNLPYNVGTAILERLLPHHERVPRAAFLLQKEVVERLTARPGDEAYGSLSVLVAAYA
                     RATVLGKVKPGSFRPPPKVESAFVGLELHPPPLPAEEMPGFTGFVRLAFSKRRKTLRN
                     SLASGWGPSGRQKAEAVLAAAGVGERVRAEELGLGELLEIWRHAACNESAPSDI"
     gene            6529..6702
                     /locus_tag="VEL74_23845"
     CDS             6529..6702
                     /locus_tag="VEL74_23845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65637.1"
                     /translation="MSLSTVIRQYLDDKISLGRGAEELRVSRAKLERVFERLGVPKKP
                     APDSDGGESLARR"
     gene            complement(6710..7468)
                     /locus_tag="VEL74_23850"
     CDS             complement(6710..7468)
                     /locus_tag="VEL74_23850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HYG65638.1"
                     /translation="MIQTIDHGPVRELRLDRPPANALNPDLIKAVRSAVESAPGDGAR
                     ALVLSGAPGFFSGGLDVPELIHLARPAIEETWRDFYAMMRALAASAIPIAAAITGHSP
                     AGGAVMAIFCDYRVMAEGELKIGLNEVQVGIPLPPVIHAALHRLLGAHKAERLAVSGR
                     LVPAAEALRLGLVDELAAPGRVVERAVEWCAQLLALPPTAMATTRRMARADLVRLFDQ
                     SQEEIGTVADAWFSDETQGVLRGIVERLASKKKG"
     gene            7524..>7740
                     /locus_tag="VEL74_23855"
     CDS             7524..>7740
                     /locus_tag="VEL74_23855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HYG65639.1"
                     /translation="MNFEISAKVRRMAGALRRFVDEEVVPLEPLALSRGFGAARPELD
                     RLRAKARALRLFAPHLPKEWGGAGLALS"
BASE COUNT         1038 a         2704 c         2960 g         1038 t
ORIGIN      
        1 gcccggctcg gcccgcagcg ccgccttgta ggcctccacg gcgccggcgg cgtcgcccgc
       61 agcgagcttg gcgttgccca ggttgtagtg ggccgccggc gacagggcgg gcccggcctc
      121 cagggccgcc tgctccagca gggggaccgc cttgcgctcg tggccggcgg acagctgggc
      181 ggtgccggcg ttgtagcgca ccgcggggtc cttctcgtcg atccgtagcg cggtgtccgc
      241 gaggtcggcg gcccggtcgg cctccccctg cgcggcggcc tcgatcgcct cggccgtccg
      301 ctcgcggggg ttgtagagcc agcgctcgac ggccgcggga agatggagcg acggccaggg
      361 gatgtccggc agggacggca gggccgccac cgccccggcg agcaggaagg ccacggccct
      421 cacgccgcct tcctccgcgc cgcctcggcg gagggctcgc cggccgcacc cttggataga
      481 gaggtctcgc gggccggggc gaacgggccg acggcgaggt agaggagcag ggcggccacc
      541 gcggcgccga gaggccactg gaagcgctcc tcgtgggtgc tgaggctctg gctctccatg
      601 gtccgttttt ccatctggtc gatctggcgc agcaacggcg ccggatccgc cgcggcgctg
      661 gtgacccgca ggtaggcgcc gccggtggcc cgcgagaggc cctccagggt ctcctcgtgg
      721 agacggctga cgatcaggtc accggtctcg tcgcgtttga agtcacggct gccggggatg
      781 gggaccggcg ccccctccgg cgtgccgatg ccgaaagcgt ggacggtgac cccctcctcc
      841 ttcaggcggg ggagccacga gtccagccgg ccgccgtggt cttcgccgtc cgacagcacg
      901 atcagcaccc ggtgcttctc gctcccctcg ccgaacaggc ggagggcggt ctcgatcccc
      961 ggggagagct cggtgcccgg cgtcggcagc gaccccggct cgatcgtatc gagcagcagg
     1021 tccaccaccg cgccgtccag ggtgagaggg gtgaggacca tggccgcccc ctcggtctgc
     1081 accagcccca gccggttgcc cggcatcgcc tgggccaggc gccggaccag cgtccgggcg
     1141 acgtagaggc gcgacggcga gacgtccatg acccccatgg agagggagga atccaccagg
     1201 aagaccacgt cgacgccgcg gcgctccacg gtctgctcgc cgctccccca gcgcggccgg
     1261 gccagggcga gcgccgcgcc cagcaccgcc agacccaggc agaccaccga cagggtgatg
     1321 cggcgcgggt gataggccgg gagcagccgg tcccagaggc cgcgcgacgc ccaggccgcg
     1381 tcggccgcaa ggcgccggcg ccagagccag gccaccgcca gcgccgccag cggggcgagg
     1441 agggccagcc agacgaggcg gggctcggcg aagctcatgg cgatcccttc atggctcggc
     1501 ggtcaccttg agacccgcgg cggcgatggg gaggaggagg agcgacagcg ccgcccagac
     1561 cagcggggcg aacgcctcgc ggtagcggac gtagcgcttc acctggagcg gcgtcttctc
     1621 cagccggtcg atctggtcga agatccggcg cagcccctcg ccgtcggtgg ccttgtagaa
     1681 ctgcccgccg gtgcgcttgg cgatctggcg catcagctcc tcgtcgaccg gcacccgcat
     1741 catcacccgc cgcacgtact gccggccggt caccgggtcg cgctgcggga aggggaccgg
     1801 cgccacgccc gtggccgcgc cgacgccgat ggtgtagacc ttgatcccca gcccgtcgca
     1861 gaccgcggcg gcggagaccg ggtcgatctc gccggcgttg ttcaccccgt cggtgaccag
     1921 cagaacgacg cgggtcttgg cctcgctgtt cttgaggcgc gccgcggcgt tggccagggc
     1981 gacgccgatc gcggtgccgt cgggcagggt gttgagctcg atcgagctga tgagctgccg
     2041 gagcatctgc cggtcggtgg tcaggggggc ccgggtgagg gcgctgccgg cgaagacggt
     2101 catgccgatc cggtcgccgg accgcccctg cacgaagtcg gtgacgacct gcttggcgac
     2161 cgtcagccgg ttgttgggct ggaagtcctc ggcccccatg ctgcccgaga tgtcgaggac
     2221 gatctggatg tcgatcccct cggtgaggct ctcctcccag gcgtagccga gctggggccg
     2281 ggcgagggcc gcgaccagga gccccaaggc gagaagccgg gcatagaacg gcaggtggag
     2341 gcgccaggcc cccgccgtcc gtccggcgcc ccgcggcagg tggctgtagg tgagtgcgcc
     2401 cagcgtctgt cgccggtggt gccgccaggc gaggaccggc agggcgagga gcagcagcag
     2461 ccaccagggg ctcgccagat gggggaaggc ctcgcccatc atcccgccgc ctccagcttc
     2521 gccggactct gctcgggcgg cttctcctga agcgcggggg gctggacgta catctccacc
     2581 tcgcgggcga tctcgcgggc ggtgccgatc cgctcccggg tgggctccgt gccggcgtcc
     2641 tggcgcgcga acttgaccag gtcgcacgcc cgcagcagct ccaccaggcg gcgcgccggt
     2701 gtccccggga cccgccggta gacgagctgg cgctggatct cgctggtggt gctctcggcg
     2761 gcggggaagg agagggcacg ccccaggtag tgccggagcg ccaggctgag ccgggtgtgg
     2821 accgccagcg gcgaaggctc cctttggaga ttgtccagct cgcgcagcag ctcgtcaaac
     2881 ggggggagcg gcggcgcggc gggcgccgcg gccgcggagc ggcgcgcctg gcgccagagc
     2941 aggaagagca gccccaggca gagggcggag gcggcggcga gcgtccacca gaagcgctcg
     3001 ccgagaggga gggcgcggag ggcggccggc ggcctggcct cggggttctt ctcctccgcc
     3061 ggcagcaccg agccgatctc cagggcgagg tccgggggcg tcagcgcctg cgccgtcccc
     3121 cgcgcgccgg ggagggccac ggccatcggc ggcagcgcca ccttgcccgg ccggaaggcg
     3181 gcgagcacca gccgctgccg gtaggtggcg acgcccccct cctcgcgcac cttctccggc
     3241 gcccccttct cgacgatctc ggcctcgccc cagccctcct gccaggaggg gaagcggggc
     3301 tcgccggtca ggtccgccgt ccgcaccgtc aggtcgagcg tcgcctcgac ccggtcgccg
     3361 acggtgagct gggacggcgg cgagaaggtc acggtgagcc gcggctcggt ggcggtcgag
     3421 ggcgcggggg cctgggcgac gagcggcgcg ccaaccagga gcatcaggag gagggggtag
     3481 agcgccctca ccgccgcagc ctccgccggc gctgctcgaa gaaggagagc accgtgccca
     3541 gataggggcg gtcggtccgc agctccaggt ggtcgacgcc gatccgccgg ctgagccggg
     3601 tgaggccggc gcgctcctgg cgccgctgct cggcgtgctt cttggccagg cgccggccgt
     3661 tgaccagggc gaggcgcccg gtctcggcgt cgcgcagcac caccggcccc acggacggga
     3721 tctcctggtc ccgcgggtcg acgatctcca ccaggatgag gtcgtggcgc gccgccgcgg
     3781 ctttgagcgc ccgctcgggc agggcgccga ggaagtccga gatcacgaac agcaccgagc
     3841 gccggcgcag cgccgcgagc acgccgtgca ggccgcgctc caggtcggtg ccgccgtgcg
     3901 ccggacggtc gagcacctcg cgcaccaggc gcagcacgtg ggtgcggcgc cgctgcggcg
     3961 gcaggaacag ctccaggtgg tcgctcacca gggcggcgcc cacccggtcc tggttgcgca
     4021 ccgcggcgaa ggcgagcagc gcggcgatct cggccgccag ctcgcgcttg aggatggccc
     4081 gcgagccgaa ggagaggctg ccggagacgt ccaccaccag gaagacggtg aggtcccgct
     4141 cctcgacgta cttcttgatg aagggggagc ccatgcgggc cgtgacgttc cagtcgatgg
     4201 tgcggatctc gtcccctggc tggtaggggc ggacctcggc aaattccata ccgcgcccct
     4261 tgaacaccgc gtggtagtcg ccggccaccc cctggtccac caggcggttg gtggcgatct
     4321 cgatccgccg caccttgcgc atcaggtcgg cggggagctg ccccgaggac ggagcgttgg
     4381 ggcgctccgc cggagccttc ttgcgcgatg agaagaggcc gaacatcggg gaccctgctc
     4441 cagcgctagg gcaccgggac gcggtcgagc aggcgcttga cgaggtcgtc cgcggagatc
     4501 tcctcggcct cggcctcgta cgtcgggatg atccggtgcc gcaggatgtc cggcgccagc
     4561 tccttgacgt cctcggggat gacgaagccg cgcccccgca ccatggccag cgccttggcc
     4621 gcccgggcga ggaacagcgt cgcccgcggc gaggcgccga aggagatgag gtcggacagc
     4681 tcgcccagcc ccaccgaccg cgggtcgcgg gtggcggtca ccaagtcgat gatgtagccg
     4741 cgcagcttct tgtcgagata gacggagtcg acgacggcgc gcagggcgag cagccggtcg
     4801 cgctccagga ccggcgccgg ccgcaaatcg ttctccacca gcatgcggtc gaggatctcg
     4861 gcctcctcgg cgcggctcgg gtagcccacc ctgagcttca tcatgaagcg gtcgacctgg
     4921 gcctcggcca ggggataggt tccctcctgc tcgatcgggt tctgggtggc gagcaccatg
     4981 aacggctcgg gcagcggcca ggtccggtcg ccgagggtga cctgccgctc ctccatggac
     5041 tccaggaggg cggactgcac cttggcgggg gcgcggttga tctcgtcggc gaggagcaga
     5101 tgggtgaaga tcggcccctt gcgcggcagg aagtcgccgc tgcgctggtc atagatcagg
     5161 gtgccgatga ggtcggccgg cagcaggtcc ggggtgaact ggatgcgctg ggcatcgatc
     5221 gccagcgtct gggccagggt tttgaccgcc agggtcttgg ccaggccggg cacgccctcg
     5281 atgagcacgt ggccgcgggc gaggaggccg acgagcaggc ggtcgagcag gtagtcctgc
     5341 ccgacgatga ccttgcgcag ctcggcctcg atccgcttga gggtgagggt ctctcgttcg
     5401 acgtcgcggt tcagtgtagg gacatccagt tccattcgtt ggctccgcca gacggtccag
     5461 cggaggccct ggggtgcaag tgaagtgcca cgcgaggttt acgggggaag aaggggctcg
     5521 ggccggaggt gcgggagccg gcccggcgct ttcccggggc ttctccggcc aatttggcac
     5581 ggctccacct tctgatagcg ttcgccgggt gagccgtccg ccgctcaaga agtcgctggg
     5641 ccagcaccac ctgatcgacg gtgcgctgac ccgccccctg gtacggttcc tggctccgct
     5701 gccgacggaa gcgggacggg tcctggagat cggcccgggg ggcggcgtgc tgaccgccga
     5761 gctgttggag gccggggcgc gggtggtggg ctgggagctg gacctccagt gggccgcctt
     5821 cctggggagg cgcttccggg cggcgcccct ctcgctggtg gtgggggacg ccctggagat
     5881 cgcgtggggc cgcctgcctg tgcccacgct cgtcgccggc aatctcccgt acaacgtggg
     5941 gaccgccatc ctggagcgcc tgctgccgca ccacgagcgg gtgccccgcg ccgctttcct
     6001 gcttcagaag gaggtggtcg agcggctgac cgcccggccc ggcgacgagg cctacggctc
     6061 gctgagcgtg ctggtggcgg cctacgcccg ggcgacggtc ctcgggaagg tcaagcccgg
     6121 cagcttccgg ccgccgccca aggtggagag cgccttcgtc gggctggagc tccacccgcc
     6181 gccgctgccg gcggaggaga tgcccgggtt caccggcttc gtccgcctcg ccttttccaa
     6241 gcgccgcaag accctgcgca actcattggc ctcggggtgg ggcccctcgg gacgacagaa
     6301 ggccgaagcc gtgctcgccg cggccggcgt gggggagcgg gtacgggccg aggagctggg
     6361 cctcggcgag ctcctggaga tctggcggca cgcggcatgt aatgaatcgg ccccctccga
     6421 catatagaga aggagaaggg tgtgttccgg gatggatccg gcaccccgag tctcctcgtc
     6481 cccccgaacc ggtgtagtct tgaggggaac ggaggcagtg gattgagcat gagcctgtcg
     6541 accgtcatcc gccagtatct cgacgacaag atcagcctcg gccggggcgc cgaggagctg
     6601 cgcgtctccc gcgccaagct cgagcgggtc ttcgagcgcc tgggagttcc caagaagccc
     6661 gctccggact cggacggcgg cgagagcctc gcccgccgct aggcccggat caccccttct
     6721 tcttgctggc cagccgctcc acgatgccgc ggagcacgcc ctgggtctcg tcgctgaacc
     6781 aggcgtcggc cacggtgccg atctcttcct gggactggtc gaacaggcgg acgaggtcgg
     6841 cccgggccat ccggcgggtg gtggccatgg cggtgggggg gagggcgagg agctgcgcgc
     6901 accactcgac cgcccgctcc accacccggc cgggggcggc gagctcgtcg accaggccga
     6961 ggcggagggc ctcggccgcc ggcaccagac ggccgctgac ggcgagccgc tccgccttgt
     7021 gcgctcccaa caggcggtgc agggcggcgt ggatcaccgg cggcagcggg atccccacct
     7081 gcacctcgtt gaggccgatc ttgagctccc cctcggccat cacccggtag tcgcagaaga
     7141 tcgccataac ggcgccgccc gccgggctgt ggccggtgat ggcggcggcg atcgggatgg
     7201 cggaggccgc gagggcgcgc atcatggcgt agaagtcgcg ccaggtctcc tcgatggccg
     7261 gccgggcgag gtggatgagc tcggggacgt cgaggccgcc ggagaagaag cccggggcgc
     7321 cggagaggac gagggcgcgg gcgccgtcgc cgggggcgga ctcgacggcg gaccgcaccg
     7381 ccttgatcag gtccgggttc agggcgttgg ccggcgggcg gtcgagccgc agctcgcgca
     7441 ccgggccgtg gtcgatggtc tggatcatga ccgctagcct accaccgggg tatccttgag
     7501 ccctccgacc ctggaggccc gcgatgaact tcgagatctc cgccaaggtc cgccgcatgg
     7561 ccggcgcgct gcggcgcttc gtcgacgagg aggtggtgcc gctcgagccc ctggcgctgt
     7621 cgcggggctt cggcgccgcc cggccggagc tggaccgcct gcgcgccaaa gcccgggcgc
     7681 tgcgcctctt cgccccccac ctgcccaagg agtggggggg cgccggcctc gccctctcgg
//
LOCUS       DASPJI010000846        10471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_113150, whole genome shotgun sequence.
ACCESSION   DASPJI010000846 DASPJI010000000
VERSION     DASPJI010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 10471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10471)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10471
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113150"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="VEL74_23860"
     CDS             complement(<1..1308)
                     /locus_tag="VEL74_23860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYG65640.1"
                     /translation="MKAPSPFPTLAGLALLSLSLTACWSILPSKGGGQTSFTGERHIA
                     PGDIALPAGYRAEMVARGLTFPTGVAFDDRGGVYVLEAGYSYGEVWTTPRLLRVGPDG
                     TASPVATGENPPWNGVAFANGAFYVSEGGEKNGGRILRITPDGQVSAVVEGLPSLGDH
                     HTNGPAVSRDGWLYFGQGTATNSGVVGPDNFEFGWLKRHPEFHDIPCRDIRLAGRNFT
                     SDNPLTPEEGDQAETGSYVPFGTRTSPGQTIPGRVPCSGAVLRVRPEGGTPELVAWGF
                     RNPFGLAFSPDGRLYLTENGADQRGSRPIYGAPDVLWEVRPGSWYGWPDHAMGKPLKD
                     GEFHQGNNPPLESLLAENPGEPPQPAARLGVHTSANGLDFSRNDAFGHVGEAFIAHFG
                     DMAPKVGKVMGPVGFKVSRVDVRTGRSEDFAVNRAGKGPASRVG"
     gene            1454..2398
                     /gene="rnhA"
                     /locus_tag="VEL74_23865"
     CDS             1454..2398
                     /gene="rnhA"
                     /locus_tag="VEL74_23865"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF001236.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HYG65641.1"
                     /translation="MIETPSATPEVTIYTDGGADPNPGPGGWGAVLLGTQGQRKELRG
                     GEPHSTNNRMELTAAIRSLEALKERCRVRLFTDSLYLRKGITQWLPGWIAQGWRRKTG
                     ELQNEDLWRRLAELVREHDIEWSWVKGHAGNRWNERADQLATQAIQEQRAARKGAARP
                     AGTSTEPVLPEVKPDVDVFLRISCARGRGGWAALLREKGDEKVLSGAATGVTPNQLDL
                     LAAAQVLESLPEGIRVALHTGSDYLRNGATAWLEGWKRRGWKTQEGNPVSNRDLWERL
                     ERAMTRRQVTWPDVKGQNLPELDALGQTAKEAAGPPRP"
     gene            complement(2426..3829)
                     /locus_tag="VEL74_23870"
     CDS             complement(2426..3829)
                     /locus_tag="VEL74_23870"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HYG65642.1"
                     /translation="MTRLLVVEDDEKIRAGLLLQLREEGYAPQSVTRAEEALAALSDP
                     HQTLPDLLLLDVRLPGMSGVDLVRELAAAGRLPPTVIVSGEASISETVEALRLGVHDF
                     IEKPFSRERLLRSIENALEHAALRREVASLKGEPEILGTSPAIEALRQRIARAAATDA
                     RILISGESGTGKELVAAALHRQSRRAQRPFIRINCAAIPGPLIEDELFGHARGAFTDA
                     KTAKPGLFEEADGGTLFLDEIGDMELPVQARLLRVLEDGRVKRVGETRDRQVDVRVLA
                     ATHRNLEEEVRAGRFREDLYFRLAHLPLPVPSLRERGADVRLLFFHFLERFCRQHRSR
                     PRRVEPEVLACLERYPWPGNVRELRNLCERLVVFGGDPVTVEDLPSSFFEPSRAPETG
                     LLLRAAPLPVMPLRELKAQCEREYIESVLVRTRWNFTAAARLLDVQRTYLHQKAKALG
                     IHRPGLEDGEEEGEREG"
     gene            complement(3826..5046)
                     /locus_tag="VEL74_23875"
     CDS             complement(3826..5046)
                     /locus_tag="VEL74_23875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HYG65643.1"
                     /translation="MTWRIAAATLGGMLLAAAAVLWIFERQISNAWFRLGHHPGTVAA
                     LERSLADQKELARLDPQRRDHYRSRFDEIQRLLQRLRIVEHNRAEIVRRYEMVLLAAV
                     GGVLLAAGGVQVARQRRQERRLQRLRDALVALAAGGEGLEVGVGGRDTLGRIAGMVED
                     ISRAVARDRRRLASLNHLSAWQEAARRHAHEMKTPLTAAHLELQRLQRDGASAAEVRQ
                     AAASLAEELERLARFTRHFTSFARLPRPRLGEHDLGEAVAEFAAAFAWAWPGLTLNLQ
                     TSLPAAAPVRAAFDREMLRQVLVNLCDNSARALSEAGREGNVEIRIMETADAAILEVA
                     DDGPGIPPAVRPTLFEPYVTTRAVGEGMGLGLAISRKILLDHGGDLELVETATPGAVF
                     RLTLSKRVRQGTAA"
     gene            complement(5043..5708)
                     /locus_tag="VEL74_23880"
     CDS             complement(5043..5708)
                     /locus_tag="VEL74_23880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65644.1"
                     /translation="MRRTVLGCLGMALLLAARLHAAPALEPAAGGGVALAGLPPLFGR
                     PEIREQLGTGLTTTVILEVKAAAPRTGTGSRPRGGARVDVRYELWDEVYLVTRVDAAG
                     KVARATLPSLEKLAEWWRDQRIVVLRAPGLAAAQGPWRVDVRLRVLPFSQAEQRDAQR
                     WLSQTLSQGGDGGSAGAASEASKDRSSSFDQVLQTLLATSIGRPALFESAWKLSYPRE
                     AGR"
     gene            complement(5705..7138)
                     /locus_tag="VEL74_23885"
     CDS             complement(5705..7138)
                     /locus_tag="VEL74_23885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65645.1"
                     /translation="MTVLRCYIFILFAGWLLLAAPPARSQELPAEGLQPEELQAEEPA
                     SFLLETITVEGAQPAAAHIVRAESLLEEGRTYTEEELRQAVYRIQRLPIVLETSFALR
                     KGGTRGAYELVIQVRQARRFFYEHGARVSFFRSDLALNEIRGEETTFGASGLVGLRQF
                     VGRTGVLVGALDTARGAQVGYSQYDLFGRGIVASVAYSWIPNVTEVLSYGLDPGFIAW
                     DVDEQNRLTFNLAVPLDASQSFQLGFSDRRGDTGQRRRILEPDLTESLRPEGSLRADE
                     LAEQRLVARWVYDTSDDPLLPTRGLSLSGGIEAASLTLRGLERFRFVQDASGFRDELI
                     PQPDYEAETVSAVFTAARHWPVTPRQTVSVSGRASAGRSWLENAQIPGDPVAFVQEDL
                     DVNVYGLSAGVRHAVRLWSGRQPRGFGDLSLESAVEAGRETTSPDLSLATTPLTRLEV
                     STGLVFRHQWGVLRFHLAYLDFREDER"
     gene            7456..9096
                     /locus_tag="VEL74_23890"
     CDS             7456..9096
                     /locus_tag="VEL74_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HYG65646.1"
                     /translation="MARTPLFRMIQRSLAMAQLSRRTGEPPRELLDRWHEARLSRRQL
                     LGGAALATAGLALSSCGPRRTGEPSSQGTAEGKVRPATKGEVLIIGAGIAGLTAAYRL
                     HQAGVPVRVIEGQERTGGRMYSSKGRFPEGQVIELGGELIDTNHTHMQELAAELGIPL
                     DDFDKDDPAMARDVWFFDGRRYSDAEVVEAFRPIAAKIDEAWETITGETVDYRQPNNG
                     ETLDNMTIADFLDEAGADGWFRDLLDVAYTTEYGREIAEQSSFNFLMMIDTNPEPFRI
                     FGESDERFHVRGGNDQIPHALAKALGDRVETGTKLEALSRAADGTYRASVRRGQTSQD
                     LTAERVVLAIPFTLLREVKLDLPLPDVKRKAIQELGYGTNAKLMVGFSDRVWRAEAKS
                     NGSVVTDLPFQLTWETTRLQPGKAGILVNFTGGQHGLDIGQGSEAQQAQAFVRDLERI
                     FPGVAERRTGEARFHWPTFPWMKGSYACYLPGQWTTIAGAEGERVENLHFCGEHTSID
                     FQGFMEGGCESGTRVAQEILTDLGLAKPEEAEGEQKVA"
     gene            9577..>10471
                     /locus_tag="VEL74_23895"
     CDS             9577..>10471
                     /locus_tag="VEL74_23895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65647.1"
                     /translation="MTKAKIAGGAFATVLVLLALAAGYRAFQPDAEPGAAASPAATPS
                     TSPSPSPSAAAAEAHQSFLYGRVTAVGGATYEGRLRWGEGGGQEAFWGDYFNGSKEEN
                     PWAAHVPLERLPKERHPIEIFGIELVQRERPIDLGRLFMARFGDITRVEGSVSGVRVT
                     LKSGTVFDLNRNAASDLDDGVRVWDATRGVIDLDSRLIKTIELLPAPRSGAAPSRLHG
                     TVRTRQGDFTGFVQWDREEGVGSDELNGFTGDSELSLRFATIRSITRRSGESSLVTLL
                     DGREVVLSGTSEAGDGNRGIYV"
BASE COUNT         1551 a         3771 c         3766 g         1383 t
ORIGIN      
        1 gcccacccgc gacgccggcc ccttgccggc ccggttcacc gcgaagtcct cgctgcggcc
       61 ggtcctgaca tccacccgcg acaccttgaa gccgaccggg cccatgacct tgcccacctt
      121 gggagccatg tcgccgaagt gagcgatgaa ggcttcaccg acatggccga aggcgtcgtt
      181 gcgcgagaag tcgaggccgt tggccgaggt gtggacgccc agccgcgccg ccggctgggg
      241 cggctcgccg gggttctcgg ccaggaggga ctcgagcggc gggttgttcc cctgatggaa
      301 ctcgccgtcc ttcaacggtt tgcccatggc atggtcgggc cagccgtacc aggagccggg
      361 gcggacctcc cagagcacgt cgggtgcgcc gtagatgggc cggctccccc gctggtcggc
      421 gccgttctcc gtcaggtaga gccggccgtc gggggagaag gcgagcccga aggggttgcg
      481 gaacccccag gccaccagct cgggcgtccc tccttcgggc cggacgcgca gcaccgcgcc
      541 cgagcaaggg acccggccgg ggattgtctg ccccgggctg gtgcgggtgc cgaaaggcac
      601 gtaggagccg gtctctgcct ggtccccctc ctcgggggtg agcgggttgt cgctggtgaa
      661 attcctgccc gccagccgga tgtcccggca agggatgtcg tggaactcgg gatggcgttt
      721 cagccagccg aactcgaagt tgtcggggcc gacgacaccc gagttggtgg cggtcccctg
      781 gccgaagtag agccagccgt cgcggctcac cgccgggccg ttggtgtggt ggtcccccag
      841 gctgggcaga ccctcgacga cggccgagac ctggccgtcc ggggtgatcc gcaggatgcg
      901 tccgccgttc ttttctcccc cctcggacac gtagaaggcg ccgttggcga aggccacgcc
      961 gttccagggc gggttctcgc ccgtggccac cggcgaggcg gtgccgtccg gacccacccg
     1021 cagcaggcgg ggggtggtcc agacctcgcc gtaggagtag cccgcctcca gaacgtagac
     1081 gccgccgcgg tcgtcgaagg cgacgccggt ggggaaggtg agcccccgcg ccaccatttc
     1141 ggcccggtag cccgcgggca gagcgatgtc gccaggggcg atgtggcgct cgccggtgaa
     1201 cgacgtctgg ccgcctccct tggaggggag gatggaccag caggctgtga gcgataggga
     1261 gaggagggcg agaccggcga gggtcggaaa cggcgaaggc gccttcatgc aggatctcct
     1321 cggaggatga cggacacctt gggggccgtc cgcttcagag gggcagcaac cgctgtgcca
     1381 gtcggtagga gagcggtcgc gcttgccccg gggccggagg tgacgcaaga tacgccgcct
     1441 ggaaccgaca accatgatcg agaccccttc agcgactccc gaagtcacca tctacaccga
     1501 cggcggcgcc gaccccaacc cggggccggg cggctggggc gcggtcctcc tcggcaccca
     1561 aggccagagg aaggagctgc gcggcggtga gccccactcg accaacaacc gcatggagct
     1621 cacggcggcc atccgctcgc tcgaagcgct gaaggagcgc tgccgggtcc gtctgttcac
     1681 cgactccctc tatctgagga aggggatcac ccagtggctc cccggctgga tcgcccaggg
     1741 ctggcgccgc aagaccggcg agctccagaa cgaggacctc tggcgccggc tggcggagct
     1801 ggtgcgcgag cacgacatcg agtggagctg ggtcaagggg cacgccggca accgctggaa
     1861 cgagcgcgcc gaccaactcg ccacgcaggc gatccaggag cagcgggcgg ctcggaaggg
     1921 ggcagcgcgg ccggcgggga cctccacaga gcccgtcttg cctgaggtga agcccgacgt
     1981 ggacgtcttc ctgcggatct cctgcgccag gggtcgaggc gggtgggccg ccctgctgcg
     2041 ggagaagggg gacgagaagg tcctctccgg cgcggccacg ggcgtcaccc cgaaccagct
     2101 cgacctcctg gccgccgccc aggtgctgga gagccttccc gagggaatcc gggtggcgct
     2161 ccacaccggc tcggactacc tgcgaaacgg cgccaccgcc tggctcgagg ggtggaagcg
     2221 gcgcggctgg aagacccagg aggggaaccc ggtctccaac cgcgacctct gggagcgcct
     2281 ggaacgggcc atgacccgcc gccaggtgac ctggcccgac gtcaagggcc agaacctgcc
     2341 cgagctcgac gccctgggcc agacggccaa ggaggcggcg gggccgcctc ggccgtgatc
     2401 tcctggagcc acgcgtagag tctcctcacc cctcccgctc cccctcctcc tccccgtcct
     2461 ccagcccggg ccggtggatc cccagggcct tcgccttctg atgcaggtag gtgcgctgga
     2521 cgtccagcag ccgggcggcg gcggtgaagt tccagcgggt gcggaccagg acgctctcga
     2581 tgtactcgcg ctcgcactgg gccttgagct cccgcaacgg catcacgggc aggggcgccg
     2641 cccgcagcag cagcccggtc tcgggggccc gcgacggttc gaaaaaggag gaggggaggt
     2701 cctccacggt gaccgggtcg ccgccgaaga ccaccagccg ctcacagagg ttccgcagct
     2761 cgcggacgtt gcccggccag gggtagcgct cgaggcaggc gagcacctcc ggctccaccc
     2821 ggcgcgggcg cgaccggtgc tgccggcaga agcgctccag gaaatggaag aagaggagcc
     2881 gcacgtccgc gccccgctcg cgcagcgacg ggaccggcag ggggaggtgg gcgagccgga
     2941 aatagaggtc ctcgcggaag cggccggcgc ggacctcctc ctcgaggttc cggtgggtgg
     3001 cggcgagcac ccgcacgtcc acctgccggt cgcgggtctc gcccacccgc ttcacccggc
     3061 cgtcctccag cacccgcagc agccgcgcct ggaccggcag ctccatgtcg ccgatctcgt
     3121 cgaggaacag ggtgccgccg tcggcctcct cgaacaggcc gggcttggcg gtcttggcgt
     3181 cggtgaaggc gccgcgggca tggccgaaca gctcgtcctc gatcagcggc cccgggatgg
     3241 cggcgcagtt gatgcggatg aacggccgct gtgcccggcg gctctggcgg tggagcgccg
     3301 cggccaccag ctccttgccg gtgccgctct cgccggagat caggatgcgg gcgtcggtcg
     3361 ccgccgcccg ggcgatccgc tgccgcagcg cctcgatcgc cggcgaggtg ccgaggatct
     3421 ccggctcccc cttgagcgag gccacctcgc gccgcagcgc cgcgtgctcc agcgcgttct
     3481 cgatggagcg cagcaggcgc tcgcggctga acggcttctc gatgaagtca tgcaccccga
     3541 gccgcagcgc ctccaccgtc tcgctgatcg acgcctcccc cgagacgatc accgtcgggg
     3601 ggagccgccc cgccgccgcc agctcccgca ccaggtcgac gccgctcatg ccggggagcc
     3661 gcacgtcgag gagcagcagg tcggggagcg tctggtgcgg gtcggagagc gcggcgagcg
     3721 cctcctcggc gcgggtcacc gactgcggcg cgtagccctc ctcgcggagc tgcagcagga
     3781 ggccggcgcg gatcttctcg tcgtcctcga cgaccagaag ccgggtcatg ccgccgtccc
     3841 ctgccggacc cgcttcgaca aggtcagccg gaacaccgct cctggagtgg cggtctccac
     3901 cagctccagg tcgccgccgt ggtcgagcag gatcttgcgg gagatggcga gtccgagccc
     3961 catcccctcg cccacggcgc gggtggtgac gtagggctcg aacagggtgg ggcggacggc
     4021 cggggggatt ccgggcccgt catcggccac ctccaggatg gcggcgtcgg cggtctccat
     4081 gatccggatc tccacatttc cctcccggcc cgcctccgac agggcgcggg cgctgttgtc
     4141 gcagaggttg acgagcacct gccgcagcat ctcacggtcg aaggcggcgc gcaccggtgc
     4201 ggcggcgggc agggatgtct gaaggttgag cgtcaggccg ggccaggccc aggcgaaggc
     4261 ggcggcgaac tcggcgaccg cctccccgag gtcgtgctcc cccaggcgcg gccgggggag
     4321 ccgtgcgaag gaggtgaagt ggcgggtgaa gcgggcgagc cgctccagct cctcggcgag
     4381 gctggccgcg gcctgccgca cctcggcggc ggaggcgccg tcccgttgca gccgctgcag
     4441 ctccaggtgg gccgcggtga gcggcgtctt catctcgtgg gcgtggcggc gggccgcctc
     4501 ctgccaggcg gagaggtggt tgagggatgc cagacggcgg cggtcccgcg ccacggcccg
     4561 cgagatgtcc tcgaccatgc cggcgatgcg gcccagggtg tcgcggccgc ccacgccgac
     4621 ttccagcccc tcgccgccgg ccgcgagcgc caccagggcg tcccgcagcc gctggaggcg
     4681 ccgctcctgg cgccgctgcc gcgccacctg gacgccgccg gccgcgagca gcacgccgcc
     4741 gaccgcggcg aggagcacca tctcgtagcg gcggacgatc tccgcccggt tgtgctcgac
     4801 gatgcggaga cgttgcagca accgctggat ctcgtcgaag cggctccggt agtggtcccg
     4861 gcgttgcgga tcgaggcgcg ccagctcctt ctggtcggcg agcgagcgct ccagcgcggc
     4921 caccgtcccg gggtggtggc cgagccggaa ccaggcgttg gagatctgcc gctcgaagat
     4981 ccacaggacc gcggcggcgg ccagcagcat gccgccgagg gtcgccgccg cgatccgcca
     5041 ggtcatcgcc ccgcctcccg cgggtaggac agcttccagg cggactcgaa gagcgccggc
     5101 cggccgatgg aggtggcgag cagcgtctgc aggacctggt cgaacgacga ggagcggtcc
     5161 ttgctggcct cggaggcggc gccggcgctg cccccgtcgc ccccctgcga cagtgtctgc
     5221 gagagccagc gctgggcgtc gcgctgctcc gcctgcgaga aggggaggac gcgcagccgc
     5281 acgtcgaccc gccagggacc ctgcgcggcg gcgagccccg gcgcccggag caccacgatc
     5341 cgctggtcgc gccaccactc cgccagcttc tccagggagg ggagcgtggc gcgggcgacc
     5401 ttgccggcgg cgtcgacgcg ggtcaccaga tagacctcgt cccatagctc gtagcggacg
     5461 tccacccgcg ccccgcctcg cgggcggctc cccgtgccgg tgcgaggagc ggccgccttc
     5521 acctcgagga tcacggtggt ggtgaggccg gtgccgagct gctcgcggat ctccggccgg
     5581 ccgaacagcg gtggcaggcc ggccagcgcc acgccgccgc cggccgccgg ctcgagggcc
     5641 ggcgcggcgt ggaggcgcgc ggcgaggagc agcgccatcc caaggcatcc cagtacggtc
     5701 cgcctcaccg ctcgtcctcc cggaaatcca ggtaggcgag gtggaaccgc agcacccccc
     5761 actggtgccg gaagaccagg ccggtcgaga cctccagccg ggtcagcggc gtggtggcga
     5821 ggctcaggtc cggcgaggtg gtctcgcggc cggcctccac cgcgctctcc aggctcaggt
     5881 cgccgaaccc ccggggctgc cggccgctcc agagccgcac ggcgtgccgg acccccgcgg
     5941 acaggccgta gacgttgacg tccagatcct cctgcacgaa ggcgaccggg tcgccgggga
     6001 tctgcgcgtt ctccagccag gagcggccgg ccgaggcccg gccgctgacc gacaccgtct
     6061 gccgcggggt caccggccaa tgccgggcgg ccgtgaacac cgccgacacc gtttcggctt
     6121 cgtagtcggg ctgggggatg agctcgtccc ggaagcccga tgcgtcctgg acgaagcgga
     6181 accgctccag gccgcggagg gtgagggagg cggcctcgat cccgccggag agggacaggc
     6241 cgcgggtggg cagcagcggg tcgtcggagg tgtcgtacac ccagcgcgcc accagccgct
     6301 gctcggcgag ctcgtcggcg cggaggctgc cctcgggacg caggctctcc gtcaggtcgg
     6361 gctcgaggat ccgccgccgc tggccggtgt cgccgcgccg gtcggagaac ccgagctgga
     6421 acgactggct cgcgtcgagc ggcaccgcca ggttgaaggt gagccggttc tgctcgtcga
     6481 cgtcccaggc gatgaagccg gggtccagcc cgtaggacag gacctcggtg acgttgggga
     6541 tccacgagta ggcgacgctc gccacgatgc cgcggccgaa gaggtcgtac tggctgtagc
     6601 ccacctgcgc gccgcgcgcc gtgtccaggg cgccgacgag gacgccggtg cggccgacga
     6661 actggcgcag gcccaccagc cccgaggccc cgaacgtcgt ctcctcgccg cggatctcgt
     6721 tcaacgccag gtccgaccgg aaaaaggaga cgcgggcccc atgctcgtag aagaagcgcc
     6781 gcgcctgccg cacctggatc accagctcgt aggcgccgcg cgtccccccc ttgcgcaggg
     6841 cgaagctggt ttcgaggacg atcggcagcc gctggatgcg gtagacggcc tgccgcagct
     6901 cctcctcggt ataggtacgc ccctcctcga gcagcgactc cgccctgacg atgtgcgccg
     6961 ccgccggctg ggccccctcg acggtgatcg tctccaggag gaagctcgcc ggctcctccg
     7021 cctggagctc ctccggctgc agcccctccg ccgggagctc ctgggaacgg gccggcggcg
     7081 cggcgagcag cagccacccc gcgaagagaa tgaagatgta gcaacgcaag acggtcatcg
     7141 ggtaaccccc tccaagtcgt gccacgaggt cctccatggc gttatgcagg cccttgaagg
     7201 gcaagggggg tgccgggctg tccggagcgc cggcggcggc gcggcgcgga ggccataact
     7261 tcaagcatct caaatgtttg agcgctccac ggcggcggcg gcgcgcgccg gtccggtacg
     7321 gttcggtccg cgttgaactg tgcggagaat catacacctg ttcgcggatc cggccttgaa
     7381 aggtacggtg gaccgcggat agagttccgg cgtcctcttc tttcacacgc tcaggcgccg
     7441 cctggaggtc catcgatggc cagaaccccc ctgttccgca tgatccagcg ttccctcgcc
     7501 atggcgcagc tctcgcggcg taccggcgag cccccccggg agctcctcga ccgttggcac
     7561 gaggcgcggc tctcccgccg gcagctcctc ggcggcgcgg ctctggcgac cgccggcttg
     7621 gccctgtcga gctgcggccc ccggcgcacc ggcgagccct cctcccaggg aacggccgaa
     7681 ggcaaagtgc ggccggccac caaaggcgag gtgctgatca tcggcgccgg catcgccggg
     7741 ctgaccgcgg cctaccggct ccaccaggcg ggcgtgccgg tgcgggtgat cgaggggcag
     7801 gagcgcaccg gcggccggat gtacagcagc aaggggcgct tccccgaggg tcaggtcatc
     7861 gagctcggcg gcgagctgat cgacaccaac cacacccaca tgcaggagct ggcggcggag
     7921 ctgggcatcc cgctcgacga cttcgacaag gacgacccgg ccatggcgcg cgacgtctgg
     7981 ttcttcgacg gccgccgcta ctccgacgcc gaggtggtgg aggcgttccg gcccatcgcg
     8041 gcgaagatcg acgaggcctg ggagacgatc accggcgaga ccgtcgacta ccggcagccg
     8101 aacaacggcg agacgctgga caacatgacg atcgccgact tcctcgacga ggccggcgcc
     8161 gacggctggt tccgcgacct tttggatgtt gcctacacca ccgagtacgg ccgcgagatc
     8221 gccgagcagt cgtccttcaa cttcctgatg atgatcgaca ccaacccgga gccattccgc
     8281 atcttcggcg agagcgacga gcgcttccac gtgcgcggcg gcaacgacca gatcccgcac
     8341 gcgctcgcca aggcgctggg cgaccgggtg gagaccggca cgaagctcga ggccctgtcc
     8401 cgggcggcgg acggcaccta ccgggcgtcg gtgcggcgcg gccagacgag ccaggacctg
     8461 accgccgagc gggtggtcct cgccatcccc ttcaccctgc tgcgcgaggt gaagctcgac
     8521 ctgccgctcc ccgacgtcaa gaggaaggcg atccaggagc tgggctacgg caccaacgcc
     8581 aagctgatgg tcggcttctc ggaccgcgtc tggcgcgccg aggcgaagtc gaacggcagc
     8641 gtggtgaccg acctgccgtt ccagctcacc tgggagacga cgcgcctcca gcccggcaag
     8701 gcggggatcc tggtgaactt caccggcggc cagcacggcc tggacatcgg ccagggctcg
     8761 gaggcgcagc aggcgcaggc cttcgtccgc gacctggagc gcatcttccc cggcgtcgcc
     8821 gagcggcgga cgggcgaggc ccgcttccac tggcccacct tcccctggat gaaggggagc
     8881 tacgcctgct acctccccgg ccagtggacc accatcgccg gcgccgaggg ggagcgggtg
     8941 gagaacctcc acttctgcgg cgagcacacc tccatcgact tccagggctt catggaaggg
     9001 ggctgcgaga gcggcacccg cgtcgcccag gagatcctca ccgacctcgg gctcgccaag
     9061 cccgaggagg ccgagggcga gcagaaggtg gcgtagccgc tcatcccccg atccccgggc
     9121 gcggcggggc cctcgccgcg cccggacatt cgggtccatc gattgtcaga gaaccttccg
     9181 gagccgtttc gacccggctc atttgccgtt cgacccttct caaaccccgc gcgacccgag
     9241 ggtcgagccg ctcgaccctg ctcgtttggg attggacccg agggtcgagc cgcttgaccc
     9301 tgctcgtcgg agactcgacc ctgctcgtcg gagactggac ccgagggtcg aaacagctcg
     9361 cgacagggtc caaccggatc aaagcgccga aaaaccatga cgcgggactc ggggccagcc
     9421 acaatttcgc cacgatctcc caccgtcttg tcatgatcca gccttcggct cgccgctgcc
     9481 gaagcgtcta ctgctgacca tggccgccgt cgcgtgccta agatagaaag gccgcgacgc
     9541 ggcatgatac tcgtgtcttc tgtgggagag ccgaccatga caaaagccaa gatcgccggc
     9601 ggggcgttcg ccaccgtgct cgtcctcctc gccctcgccg ccggctaccg ggccttccag
     9661 ccggacgccg aacccggcgc ggcggcctcg cccgcggcca ccccctcgac cagcccctcg
     9721 ccttccccgt ccgcggcggc ggcggaggcc caccagagct tcctctatgg ccgcgtcacc
     9781 gccgttggcg gcgccaccta cgaggggcgg ctgcgctggg gggagggggg cggccaggag
     9841 gctttctggg gcgactactt caacggctcc aaggaagaga acccttgggc cgcccacgtg
     9901 ccgctcgagc ggctgcccaa ggagcgccac ccgatcgaga tcttcgggat cgagctcgtc
     9961 caacgggagc gcccgatcga cctcggccgg ctcttcatgg cgcgcttcgg cgacatcacg
    10021 cgcgtcgaag ggagcgtcag cggcgtgcgg gtgactctca agagcgggac cgtgttcgac
    10081 ctcaaccgca acgccgccag cgacctggac gacggcgtgc gggtgtggga cgccacgcgg
    10141 ggagtcatcg acctcgacag ccggctgatc aagaccatcg agctcctccc tgcaccccgg
    10201 tcaggtgccg ccccctcccg gctgcacggt acggtgcgca cgcggcaggg cgacttcacc
    10261 ggcttcgtcc agtgggaccg ggaggagggc gtcggctccg acgagctcaa cggcttcacc
    10321 ggcgactctg agctcagcct gcgcttcgcc accatccgct ccatcacgcg ccgctcgggc
    10381 gagagctccc tggtgacgct gctcgacggc cgcgaggtcg tgctctccgg cacctccgaa
    10441 gccggcgacg gcaaccgcgg gatctacgtc g
//
LOCUS       DASPJI010000847         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_732926, whole genome shotgun sequence.
ACCESSION   DASPJI010000847 DASPJI010000000
VERSION     DASPJI010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732926"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VEL74_23900"
     CDS             <1..623
                     /locus_tag="VEL74_23900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017300646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HYG65648.1"
                     /translation="RQALEAGFDGVEIHGANGYLVDQFLRDGSNHRTDVYGGSLENRA
                     RFLLEVTEAVVGVWGAGRVGVRLSPTASFNDMADSDPRSTFAYAAGELDRFGLAYLHV
                     VAPGSLDPATEESAVLSAIRERFHGPLMVNGGFTRETGDAILQAGLSDLVSFGVPFLA
                     NPDLPERFAEGAPLNEPDRATFYGGGERGYVDYPTRDAVAAVGAGV"
     gene            676..1437
                     /locus_tag="VEL74_23905"
     CDS             676..1437
                     /locus_tag="VEL74_23905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65649.1"
                     /translation="MRPNPLLLGLALAPLAASGPLAPGPLLAEPIRSIQYEFPADTAR
                     ALRWKTGSGAFTFGAWDEPSVLAEVRIHCAGTDEEACRREAETIQLEWLQKGDRIELG
                     VGAPPAASGDGKGGDGKGVEAGPPPAPSALTIEGRLRVPAGLPLEVNLESGSVDVSGL
                     WGDVAVELGSGDASLLLSRRRAGAVDLTSDTGTVELTVNGRRVPGDLEPRDGPRALHW
                     KGEGRSAIKVKVGTGNARVRLEDGGKLEPGGGGKP"
     gene            1452..1886
                     /locus_tag="VEL74_23910"
     CDS             1452..1886
                     /locus_tag="VEL74_23910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HYG65650.1"
                     /translation="MLRDGDPSDIDAILALFADWKEAVRARDLRRLAALVTEDAVFSA
                     PDRPPVRGRDAVEDLYRQVFATFELDQDFELQEVRVLGTWAFVVGQDVARFDSRDRDG
                     GSVSGKGMAISIVRREPDGQWRFARGINNMIRDPAVEVRLGG"
     gene            2037..>3911
                     /locus_tag="VEL74_23915"
     CDS             2037..>3911
                     /locus_tag="VEL74_23915"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HYG65651.1"
                     /translation="MTLSAPQSFVDVVRERSGRLGERRVFTFLADGEEPAEHLTYSGL
                     DLRARAVGAELQRRGAAGDRVLLLFPPGLDFVAAFLGCLYAGAVAVPAYPPSPGRGTG
                     RLRGLLQDARPRLALTVSAALDRVERELAEAAEPPVVHAIDALPPEVAGDWRPPVVDP
                     STLAFLQYTSGSTSTPKGVRITHGNLLANERAIQRAFGQSEESVVVGWLPLYHDMGLI
                     GNVLQPIWCGGSCVLMSPLAFLQKPSRWLAAVSRFRATTSGGPDFAYALCVRKVPPAE
                     RAGLDLSSWRVAFNGAEPVRAATLDAFAEAFAPSGFRRRAFYPCYGLAEATLFVSGGD
                     PDVQPVVRTVQEAGPEGRPLVGCGRVWPGERLAIVEPESGRLAPAGHEGEVWVAGPSV
                     ADGYWNRPEETERTFGARLETGEGPFLRTGDLGLLDTAGELFVTGRLKDLIVVRGRNL
                     YPHDLERTAEESHQALRPGGAAAFSAEMDGEERLVVLVEVERRQEAEAGEAAEAVRSV
                     LAREHEVTPWEVVPIRAGSLPKTSSGKVRRSASREEYLAGGLTRVAPVAVHASAAAAG
                     SRLEAVRGLAARLLRVDPQSLDPEADLPLDSLLALEMKNRLESDFGVQLPLSVLLDGV
                     S"
BASE COUNT          485 a         1384 c         1480 g          562 t
ORIGIN      
        1 cccgccaggc gctggaggcg ggcttcgacg gggtcgagat ccatggcgcc aacggctatc
       61 tggtcgacca gttcctgcgc gacggcagca accatcggac cgacgtctat ggcggctcgc
      121 tggagaaccg tgcccgcttc ctcctggagg tgaccgaggc ggtggtgggc gtctggggcg
      181 ccggccgggt cggggtgcgg ctctctccca ctgcctcgtt caacgacatg gccgactccg
      241 acccccggtc gacgttcgcc tatgccgccg gggagctcga ccgcttcggc cttgcctatc
      301 tgcacgtggt ggcgccgggc agcctggacc cggcgacgga ggagagcgcc gtgctgtcgg
      361 ccatccgcga gcgcttccac ggcccgttga tggtcaacgg cggcttcacc cgtgagaccg
      421 gcgacgcgat cctccaggcc gggctgtccg acctggtctc cttcggcgtg ccgttcctcg
      481 ccaaccccga cctgccggag cgcttcgccg agggggcgcc gctgaacgag cccgaccgcg
      541 ccaccttcta cggcggcggc gagcggggct acgtcgacta cccgacccgc gacgccgtgg
      601 ccgccgtggg cgcaggggtg taagcatgca tcgcggagca tcgtagaatc ctctggacgt
      661 ttggacggga ggacgatgcg accgaatccg cttctcttgg gtctggccct ggcgcccctg
      721 gcggcgtccg ggccgttggc gcccgggccg ttgctggccg agcccatccg cagcatccag
      781 tacgagttcc ccgcggacac cgcccgggcg ctccgctgga agaccggctc cggcgccttc
      841 accttcggcg cctgggacga gccgagcgtc ctcgccgagg tccggatcca ctgcgccggc
      901 acggacgagg aggcctgtcg cagggaggcc gagaccatcc agctcgaatg gctgcagaag
      961 ggggaccgga tcgagctcgg ggtcggcgcc ccgcccgccg cctcgggcga cggcaaaggc
     1021 ggcgacggca aaggtgtcga ggccggcccg ccgccggcgc cgtccgctct caccatcgag
     1081 ggccggctgc gggtgccggc cggcctgcct ctcgaggtga acctggagag cggcagcgtc
     1141 gacgtctccg ggctttgggg cgacgtggcc gtggagctgg ggagcggcga cgcctccctg
     1201 ctgctctccc ggcggcgcgc cggggcggtc gacctgacct cggacaccgg cacggtcgag
     1261 ctgacggtca acggccgccg ggttccgggg gacctcgagc cccgcgacgg cccgcgcgcg
     1321 ctccactgga agggggaggg gaggtccgcc atcaaggtga aggtcggcac cggcaacgcc
     1381 cgggtccggc tggaggatgg cggcaagctc gagcccgggg gcggagggaa gccctgatgg
     1441 aggcggccgg catgctccgg gacggcgacc cgtccgacat cgatgcgatc ctggcgctct
     1501 tcgcggactg gaaggaggcg gtgcgggcgc gggacctccg gcggctggcc gccctggtca
     1561 ccgaggacgc ggtgttctcc gcgcccgacc ggccgccggt gcgcggccgg gacgccgtcg
     1621 aggacctcta ccggcaggtg tttgccacct tcgagttgga ccaggacttc gagctgcagg
     1681 aggtccgggt gctcggcacc tgggccttcg tggtcggcca ggacgtcgcc cgctttgact
     1741 cccgggaccg cgacggcggc agcgtcagcg gcaaggggat ggccatctcc atcgtccgcc
     1801 gcgagccgga cggccagtgg cggttcgccc gcggcatcaa caacatgatc cgggaccccg
     1861 cggtcgaggt ccggctcggc ggctgagccc ggaccccgca ccgagcagtc ccgccagctc
     1921 cgctacacag ttccaggatt tactggatac ctgccgacag tacgttcttc gcacgcctga
     1981 ttcatgcgca tgtcacaata ggggcgaccc cctgccgggg agaggagccg aacgtcttga
     2041 ccctgtccgc gccccagagc ttcgtcgacg tcgtccgtga gcggtccggc cgcctggggg
     2101 agaggcgcgt cttcacgttc ctggccgacg gggaggagcc ggcggagcac ctcacctatt
     2161 cggggctcga cctcagggcg cgcgcggtgg gcgcggagct ccagcgccgg ggggcggcgg
     2221 gcgaccgggt gctgttgctc ttcccgccgg ggctcgattt cgtcgccgcg ttcctgggtt
     2281 gcctctatgc cggcgccgtg gcggttccgg cctacccacc ctccccgggc cgcggcacgg
     2341 gccgcctgcg cggcctgctc caggacgccc ggccccggct ggcgctgacg gtctccgccg
     2401 cgctcgaccg cgtggagcgg gagctcgcgg aggccgccga gcccccggtg gtgcacgcga
     2461 tcgacgcgtt gcctccggag gtggccgggg actggcgccc gccggtcgtc gatccatcga
     2521 ccctggcgtt cctccagtac acctccggct cgacctcgac gcccaagggg gtgcggatca
     2581 cccacggcaa cctcctcgcc aacgagcgcg ccatccagcg cgccttcggc cagtcggagg
     2641 agtcggtcgt ggtgggctgg ctaccgctct accacgacat ggggctgatc gggaacgtgc
     2701 tgcagccgat ctggtgcggc gggagctgtg tgctgatgtc tcccctcgct ttccttcaga
     2761 agccgtcgcg gtggctggcg gccgtcagcc gcttccgcgc gacgacgagc ggcggccccg
     2821 acttcgccta cgcgctctgt gtgcgcaagg tgccgcctgc cgagcgcgcg gggctcgacc
     2881 tgtcgagctg gcgcgtggcg ttcaacggcg ccgagccggt gcgggcggcg acgctcgacg
     2941 ccttcgccga ggccttcgcg ccctccggct tccggcgccg ggccttctat ccctgctacg
     3001 gcctggccga ggccacgctg ttcgtcagcg gcggcgaccc ggacgtgcag cccgtcgtgc
     3061 gaacagtgca ggaggcgggg ccggagggga ggcccctggt cggctgcggc cgggtctggc
     3121 ccggggagcg gctggcgatc gtcgagccgg agtccgggag gttggcgccg gccggccacg
     3181 agggggaggt ctgggtggcc ggtcccagcg tcgccgacgg ctactggaat cgccccgagg
     3241 agacggagcg gacgttcggc gcccggctgg agaccggcga aggccccttc ctccgtaccg
     3301 gcgacctcgg gctgctggac acggcggggg agctgttcgt caccggccgg ctcaaggacc
     3361 tgatcgtggt ccgcgggcgc aacctctacc cgcacgacct ggagcggacc gccgaggaga
     3421 gccaccaggc tttgcgtccc ggtggggcgg cggctttctc cgccgagatg gacggcgagg
     3481 agcggctggt ggtcctggtg gaggtggagc ggcggcagga ggccgaagcc ggggaggcgg
     3541 ccgaggcggt ccgctccgta ctggcgcggg aacacgaggt cacgccctgg gaggtggtgc
     3601 cgatccgcgc cggtagcctg cccaagacct ccagcggcaa ggtgcggcgg tcggcaagtc
     3661 gggaggagta cctggccgga gggctcaccc gcgtcgcccc cgttgccgtc cacgcgagcg
     3721 ctgccgccgc ggggagccgg ttggaggccg tgcgcgggct cgccgcccgc cttttgcgcg
     3781 tagacccaca gagcctcgac ccggaggctg acctgccgct cgactcgctc ctggcgctgg
     3841 agatgaagaa ccggctggag agcgacttcg gagtccagct ccccttgagc gtgctcctgg
     3901 acggggtctc g
//
LOCUS       DASPJI010000848         7752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_567684, whole genome shotgun sequence.
ACCESSION   DASPJI010000848 DASPJI010000000
VERSION     DASPJI010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7752)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7752
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567684"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VEL74_23920"
     CDS             <1..1209
                     /locus_tag="VEL74_23920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FesM"
                     /protein_id="HYG65652.1"
                     /translation="LFCMACPFMLPRELARRVFAPVLHWPRWLRSKWLALALFVLVLF
                     AYELFDLWGSPWWTAWLIVGYFAGALAVDAVFKGAPFCKWVCPIGQFNFIASTVSPLE
                     VAVRDPDVCATCTTMDCIRGTRDPADRTRVVQRGCELALFQPKKEGNMDCTFCLDCVY
                     ACPHENVALKTRVPASELWSDRRRSSVGLFSKRKDLAALAILFTFGALLNAFGMVSPV
                     YAVQSWLAGLLGTTREAPVLGVLFVLLLVVEPAVLLGLTAWLTRKRAGTREPLLPLVV
                     RFSYALVPFGFAVWVAHYCFHLLTGLWTFVPVVQKGLADLGVTFLGAPRWDLSGLPEE
                     LVYPLELGFLGLGLLGSLLVAYRIAEREYPGRAMRASAPWWGLCFLLWISAIWLLSQP
                     MEMRGTFLGS"
     gene            1211..1765
                     /locus_tag="VEL74_23925"
     CDS             1211..1765
                     /locus_tag="VEL74_23925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65653.1"
                     /translation="MTRTVRLLILLVLSLVPIVPTAPAVAHEGPPFPIVVDHRAGPYM
                     VSIWTDPDIGTGVVYVILEPPKGMESLPPGEARVRVGVQPVTKRLPEVFYDAEAQPVR
                     HGARYFTEAKFDRGEMWRIRAVVEGPQGGGEAGAEVEATPDGTLGPGSLVLYLMPFLA
                     IGFIWLKAILRRREAAAPQPSVQR"
     gene            complement(1744..3045)
                     /locus_tag="VEL74_23930"
     CDS             complement(1744..3045)
                     /locus_tag="VEL74_23930"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HYG65654.1"
                     /translation="MSTRALLLAATLLMIARLALPLAGQETATPSSAAPAVRREIRVP
                     LDHAAPDFGTGTIRIEMARPFDPKKPTLLIVADGQQFYLLREGAVARLEQERFGDGFN
                     LVGVIGRGFSEDVVARTRRADGSVDWALAYRLLGSRQWVEDLDLVRRELVGADGKVLL
                     YGVSGGGTLVHEYLAVHGSRVTRAVSESGPVRHLDAWLRLNHDRAFWNEIQASDPRRA
                     TALSAALAKRPDRPEVIQLLQRQHYFVAVDDLAAERRRAIDEIVAGNEPAIAKRKEAY
                     QVHAMRDLLASDTGVGIRVRMYEFVEPLLRHVRIDGQAIHPNIENEAILARPLLDLQR
                     AGTLPAPPFDRRALQRVPAEVLVIGARHDQTSDYRALIANAALYPRGELFLADDNHML
                     MRLTESGARKRLLSTFLLHGLGSPEMEALLREIEPYRWTEG"
     gene            complement(3089..4882)
                     /locus_tag="VEL74_23935"
     CDS             complement(3089..4882)
                     /locus_tag="VEL74_23935"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HYG65655.1"
                     /translation="MRTRAALSLCCALLLAGSGCTSTEQGEGPSSLGAGLRDVAGEEE
                     GRGRPEDRRGVALEEVVEEGPAGLAGLRSGDRVETLDGQPIRGACELEQALAGRRPGE
                     AVRLTVRRGSDTLDKIVPLASAVDLYTAACDRGRAAACRHLGLLYFKGKGLPADPDRG
                     RALVEQACQGGDAGACGDMALLVYESGAQDQKARAVDLASRACDAGDAAGCSHLAYFY
                     STGFGVRQDESRATTYYRLACDGGDANGCFNVGLMYEKGRGVPTNNALAIFGYTRACD
                     GGSSLACTNLGFLYERGIGMTKDPERAARLYRQGCEGEPCRPGDPKSCVNLGIFYREG
                     LGVERDKAEAATFFERGCDLGNADGCADLADLLAFGDGVPKDEKRACELMRLACDAGH
                     LLSCRNLVALARTGEEPGEPGADEAAAARLQESCDGGDTTACQELGVIWDQHGLGGRR
                     DPSKAAALYRKSCDGGEGRGCVDLGVMYATGDGVPEDDARAGELYQKGCDLGQPRGCY
                     NLANFHADGTGVPQDPHRAAGLYERACEAGDMLACVNLGVMYQEGHGVLQDEKRAADL
                     YRQACQGGEKLGCSNLDLLMPDSKWRRRPNR"
     gene            5065..5964
                     /locus_tag="VEL74_23940"
     CDS             5065..5964
                     /locus_tag="VEL74_23940"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HYG65656.1"
                     /translation="MRDRLVLWYLRTALAASGRVSGFAASLHNSLLFHLGFKLHFKVR
                     PDDIFVATYPKSGTTLTQMILYQLTTDGSMDFPHVDAVMPFYELEFMRGLPARFEALP
                     SPRVFKSHLLREHLPDQARYIYVTRNLEDVAVSAYHHYCLVTGVENDFTLYTEAFAQG
                     GVPMFNSWVRHFESWWPRRNDPNVLFLRYEEMVRDLEGAVRRIAGFCGIPVDEAAMPR
                     ILERCGLEFMKRHEDKFDPRFHRISGHQGVFIRKGGTGEGAPKLSAAQQEKLEERVAA
                     LARKLGCSDRELSGTLSRSQPIG"
     gene            5961..>7752
                     /locus_tag="VEL74_23945"
     CDS             5961..>7752
                     /locus_tag="VEL74_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HYG65657.1"
                     /translation="MIQDVPSTVRGAASFVELLRARAADRPVAPLYTFLAGGEEEAGT
                     LGAAGLDLRARGLAATLQQSGAAGERALLLFPPGLDFITAFFGCLYAGAVAVPAYPPR
                     AERIDPRLEEIVRDCRPRFVLTTAAILARLRSRGAGTDLGGLQDARWIAIDEAGTTGD
                     SDAAEWQEPELGPESLAFLQYTSGSISAPKGVMVTHGNLLHNEGLIRRAFRQTESSVV
                     VGWLPLYHDMGLIGNVLQPLYSGGQAVLMSPWAFLQSPARWLRAISRYRATTSGGPDF
                     AYDLCTRRVRPEQRTGLDLSSWEVAFNGAEPVRAETLERFAAAFAPYGFRRDAFQPCY
                     GLAEATLLVSAGATGGGGGPVVLPVNPLALERGVAVESDGPAGRRLVGCGRPGEEVRV
                     VNPETAVPLGDGAVGEIWVAGPSVAAGYWERPGASAETFGARLAGEEGPPFLRTGDLG
                     FLAVGELYVTGRIKDLIIVRGRNLYPQDIERTAEGAHRALRPGGGAAFSVEVDGEERL
                     VVAAEVERRSESEAPRAAAAVRRAVVEEHEVQVHEVVLLAAGTLPRTSSGKVRRGACR
                     AAYLAGGGLEVVGRSAPAGSLGEVEEDLPGL"
BASE COUNT          979 a         2689 c         2850 g         1234 t
ORIGIN      
        1 ctcttctgca tggcctgccc gttcatgctc ccccgggagc tcgcccgccg cgtcttcgcg
       61 ccggtgctcc actggccccg ctggctgcgc tccaagtggc tggccctggc gctcttcgtc
      121 ctggtcctct tcgcctacga gctgttcgac ctctggggct ccccctggtg gaccgcctgg
      181 ctgatcgtcg gctacttcgc cggcgccctg gcggtcgacg ccgtgttcaa gggggcgccg
      241 ttctgcaagt gggtctgtcc catcggccag ttcaacttca tcgcctcgac ggtctcgccg
      301 ctcgaggtgg cggtccgcga tcccgacgtc tgcgccacgt gcaccaccat ggactgcatc
      361 cgggggaccc gcgatccggc ggaccgcacc cgggtcgtcc agcgcggctg cgagctggcg
      421 ctcttccagc cgaagaagga gggcaacatg gactgcacgt tctgtctgga ctgcgtctat
      481 gcctgcccgc acgagaacgt ggcgctcaag acgcgggtgc cggcgagcga gctgtggagc
      541 gaccggcgcc gctcgtcggt cgggctcttc tcgaaacgca aggacctggc cgcccttgcc
      601 atcctcttca ccttcggggc gctgctgaac gccttcggca tggtgagccc ggtctatgcc
      661 gtgcagtcct ggctcgccgg actcctgggg acgacgcggg aggcgccggt gctgggggtc
      721 ctcttcgtgc tgctcctggt ggtggagccg gccgtgctgc tggggctgac ggcttggctc
      781 acccggaagc gtgcgggcac gcgagagccg ctgctcccgc tggtcgtgcg gttctcctat
      841 gccctggtgc cgttcggctt cgcggtatgg gtggcccact actgcttcca cctgctcacc
      901 gggttgtgga cgttcgtgcc cgtggtccag aagggcttgg cggacctggg ggtcaccttc
      961 ctcggcgcgc cgcggtggga cttgagcggc ctgccggagg agctggtcta tccgctggag
     1021 ctgggcttcc tggggctcgg gctcctcggc tcgctgctgg tcgcctaccg gatcgccgag
     1081 cgcgagtacc ccggccgggc gatgcgggcc tcggcgccct ggtgggggct ctgcttcctc
     1141 ctctggatct ccgccatctg gctcctctcc cagcccatgg agatgcgggg caccttcctg
     1201 gggagctgag atgactcgaa ctgtccgcct gctgatcctc ctggtcctct ccctggtgcc
     1261 catcgtgccg accgcgccgg ccgtggccca cgagggcccc ccttttccga tcgtggtcga
     1321 ccaccgcgcc ggcccctaca tggtctcgat ctggaccgat ccggacatcg ggacgggcgt
     1381 cgtctacgtg atcctggagc cacccaaggg gatggagagc ctgccgccgg gggaggcgag
     1441 ggtgagggtg ggcgtgcagc cagtcacaaa gcgtctcccc gaggttttct atgacgccga
     1501 agcccagccg gtgcgccacg gcgcccgcta tttcaccgag gcgaagttcg accgcgggga
     1561 gatgtggcgc atccgcgccg tcgtcgaggg cccgcagggg ggaggggagg ccggcgccga
     1621 ggtggaggcg acgccggacg gaaccctcgg ccccggctcg ctggtgctgt acctgatgcc
     1681 gttcctggcc atcggcttca tctggctcaa agccatcctg cgccgccgcg aggcggcggc
     1741 gcctcaaccc tccgtccagc gatagggctc gatctcccgc agcagcgcct ccatctcggg
     1801 ggagccgagg ccgtggagga ggaaggtgga gaggaggcgc ttgcgcgcgc ccgactcggt
     1861 aagccgcatc agcatgtggt tgtcgtcggc caggaagagc tctccccggg ggtagagcgc
     1921 ggcgttggcg atgagcgccc ggtagtccga agtctgatcg tgacgggccc cgatcaccag
     1981 gacctcggcc gggactcgtt gcagcgcccg acggtcgaac ggcggcgcgg ggagggtgcc
     2041 ggcgcgctgg aggtcgagca gggggcgggc gaggatcgcc tcgttctcga tgttggggtg
     2101 gatcgcctgg ccgtcgatcc ggacatggcg cagcagcggc tccacgaact cgtacatgcg
     2161 gacccggatg ccgacgccgg tgtcgctcgc caggaggtcc cgcatcgcat ggacctgata
     2221 ggcctccttc cgcttggcga tggccggctc gttgccggcg acgatctcgt cgatggcgcg
     2281 gcgccgctcg gcggcgaggt cgtccacggc tacgaagtag tgctggcgct gcaggagctg
     2341 gatcacctct gggcggtccg gtcgcttcgc cagggcggcg ctcagcgcgg tggcgcgccg
     2401 cgggtccgac gcctggatct cgttccagaa ggcgcggtcg tggttgaggc gcagccaggc
     2461 gtcgaggtgg cgcaccggcc ccgactcgga gaccgcccgc gtcacccggc tgccatggac
     2521 ggccaggtac tcgtggacca gcgtgccgcc gcccgagacg ccgtagagca ggaccttgcc
     2581 gtccgcgccc acgagctcgc ggcgcacgag gtcgaggtcc tccacccact ggcggctgcc
     2641 gaggagccgg taggcgaggg cccagtccac cgagccgtcg gcgcggcggg tgcgggcgac
     2701 gacgtcctcg gagaacccgc ggccgatcac ccccaccagg ttgaagccgt cgccgaagcg
     2761 ctcctgctcg agccgggcga cggcgccctc acgcaggaga tagaactgct ggccgtcggc
     2821 cacgatgaga agcgtcggct tcttcgggtc gaacggccgc gccatttcga tgcggatggt
     2881 gccggtcccg aagtccggcg cagcgtggtc cagggggacg cggatctcgc gccggaccgc
     2941 cggcgcggcg gaggaggggg tggcggtctc ctgaccggcc aggggcaggg ccaacctggc
     3001 gatcatcagg agggtggcgg cgaggaggag ggctcgcgtg ctcatggtct ccacctacga
     3061 gaccggccgc tcgaggttgc gctgcccctc agcggttcgg tcgccgccgc catttgctgt
     3121 cgggcatcag gaggtccagg ttggagcagc cgagcttctc tcccccctgg caggcctgac
     3181 ggtagaggtc cgcggcgcgc ttctcgtcct ggaggacgcc gtggccctcc tggtacatca
     3241 ccccgaggtt gacgcaggcc agcatgtccc cggcctcgca ggcgcgctca tagaggccgg
     3301 cggcgcggtg ggggtcctgc gggacgccgg tgccgtcggc atggaagttg gctaggttgt
     3361 agcacccgcg aggctggccc aggtcgcagc ccttctggta gagctcgccg gcgcgggcgt
     3421 cgtcctcggg cactccgtcc cccgtcgcgt acatcacgcc gaggtcgacg cagccccggc
     3481 cctcgccgcc gtcacaggac ttgcggtaga gggctgccgc cttcgaaggg tcgcgccggc
     3541 cgccgaggcc gtgctggtcc cagatcaccc ccagctcctg gcaagccgtc gtgtcgccgc
     3601 cgtcgcagga ctcctggagc cgcgccgcgg cggcctcgtc ggctcccggc tcgcccggct
     3661 cctcgccggt gcgggccagg gccaccaggt tgcggcagga gagcaggtgc cccgcgtcgc
     3721 aggccagccg catcagctcg caggcccgct tctcgtcctt ggggacgccg tccccgaagg
     3781 ccaggaggtc ggcgaggtcg gcgcagccgt cggcgtttcc gagatcgcag ccgcgctcga
     3841 agaaggtggc ggcctctgcc ttgtcgcgct cgacgccgag cccctcgcga tagaagatgc
     3901 cgaggttgac gcacgacttg ggatcgccgg ggcggcaggg ttccccctcg cagccttgcc
     3961 ggtagagacg cgcggcgcgc tccgggtcct tggtcatgcc gatgccccgc tcgtagagga
     4021 agccgaggtt ggtgcaggcg agggagctgc cgccgtcgca ggcgcgggtg tagccgaaga
     4081 tggccagggc gttgttggtg gggacgcccc gccccttctc gtacatcagg ccgacgttga
     4141 agcagccgtt ggcgtccccg ccgtcgcagg ccagccggta gtaggtggtg gcgcggctct
     4201 cgtcctggcg gacgccgaaa ccggtgctgt agaagtacgc caggtgagag cagcccgcgg
     4261 catccccggc gtcgcaggcg cgggacgcca gatcgacggc ccgggccttc tggtcctgcg
     4321 cccccgactc gtagaccagg agcgccatgt cgccgcaggc cccggcgtct ccgccttgac
     4381 aggcctgctc gacgagcgcc ctgccacggt cgggatccgc gggcagtccc ttgcccttga
     4441 agtacagcag ccccaggtgc cggcaggcgg cggcccggcc gcggtcgcag gccgccgtgt
     4501 agaggtccac ggcgctcgcc aggggcacga tcttgtccag ggtgtcggag ccgcggcgga
     4561 cggtgagccg caccgcctct ccggggcggc gtccggccag cgcctgctcg agctcgcagg
     4621 ccccgcggat gggctgcccg tccagggtct cgacgcggtc gccgctccgg aggccggcga
     4681 gcccggcggg cccctcttcg acgacctcct cgagagcgac accgcggcgg tcctcgggac
     4741 ggccgcgccc ctcctcctcg ccggcgacgt cgcgcagtcc ggcgccgagg ctggagggac
     4801 cctccccctg ctccgtggag gtgcagccgg agccggcgag cagcagggcg cagcagagag
     4861 agagggcggc gcgggtccgc atcgttccct ccatcgtacc cccggactcc ggttttcccc
     4921 taccggccca cctccgggca cgccccggcc tcatcgagca ctagtagttt aggagaggcc
     4981 tacggtgccg tggcggtcca tagccagggt gctatgattg ccggccggct ttcggccagt
     5041 gaccagaggg ccggagatag ctctatgcgt gatcgattgg ttctctggta tctgcggacc
     5101 gctttggcag cctcggggag ggtctccggc ttcgccgcca gtctccataa ctcgttgttg
     5161 tttcacttag gtttcaagct ccatttcaag gtgaggccgg acgacatctt cgtcgccacc
     5221 tatccgaagt ccgggaccac gctcactcag atgatcctgt atcagctcac cacggacggc
     5281 agcatggact tcccccacgt ggacgcggtg atgccgttct acgagttgga gttcatgcgt
     5341 ggcctgccgg cccggttcga ggcgttgccg tcgccccggg tcttcaagag ccatctcctg
     5401 cgcgagcacc tgccggatca ggcgcgctac atctacgtga cccgcaatct cgaggacgtg
     5461 gcggtgtcgg cctaccacca ctactgcctg gtgaccggtg tggagaacga tttcaccctc
     5521 tacaccgaag cctttgccca gggaggggtc ccgatgttca actcctgggt gcgccacttc
     5581 gagtcctggt ggccccggcg gaacgatccg aatgtgctgt tccttcgcta cgaggagatg
     5641 gtccgcgacc tcgagggggc ggtccgccgg atcgccggct tctgcggcat tccggtcgac
     5701 gaggcggcca tgccccgcat cctggagcgg tgcgggctgg agttcatgaa gcggcacgag
     5761 gacaagttcg atccacggtt ccaccgcatc tcaggtcacc agggtgtctt catccgcaag
     5821 ggggggacgg gggagggggc cccgaagctg agcgccgccc agcaggagaa gctcgaggag
     5881 cgggtggcgg ctcttgccag gaagctcggc tgcagtgatc gggagctgtc cggaactctc
     5941 tcccgatcgc agcccatcgg atgatccagg acgtaccctc taccgtccgc ggggccgcga
     6001 gcttcgtcga gctgctgcgg gcccgggccg ccgaccggcc cgtggcgccg ctctatacgt
     6061 tcctcgcggg cggcgaggag gaggccggca ccctcggcgc ggccgggctc gacctgcggg
     6121 cgcggggcct cgccgccacg ctgcagcaga gcggagcggc gggcgagcgg gcgctcctcc
     6181 tgttcccccc ggggctcgac ttcatcaccg cctttttcgg ctgcctctac gccggagcgg
     6241 tcgccgtccc cgcctatccc ccccgggcgg agcggatcga tccgcgcctc gaagagatcg
     6301 tccgcgactg ccggccacgg ttcgtgctga ccacggcggc catcctggcc cgcctccggt
     6361 cgcggggggc cggcacggac ctcggcggtc tccaggacgc gcggtggatc gccatcgacg
     6421 aagccggcac taccggcgac tcggacgccg cggagtggca agagccggag ctcggccccg
     6481 agagcctggc cttcctccag tacacctcgg ggtcgatctc cgcgcccaag ggggtgatgg
     6541 tcacccatgg caacctgctg cacaacgagg ggctgatccg gcgggcgttc cggcagacgg
     6601 agtcgtcagt ggtggtgggc tggctgccgc tgtaccacga catggggctg atcgggaacg
     6661 tgctgcagcc gctctactcc ggcggccaag ccgtcctcat gtctccctgg gccttcctgc
     6721 agagcccggc gcggtggctg cgggcgatct cgcgctaccg cgccaccacc agcggcggcc
     6781 ccgacttcgc ttacgacctc tgcacgcggc gggtgcggcc ggagcagcgc accggtctgg
     6841 acctctcgtc atgggaggtg gccttcaacg gcgccgagcc ggtgcgggcc gagacgctgg
     6901 agcggttcgc cgcggcgttc gccccgtacg gcttccggcg cgacgcgttc caaccttgct
     6961 acggcctggc cgaggcgacg ctgctggtct cggccggcgc taccggcgga ggcggcggcc
     7021 cggtagtcct gccggtcaat cccctggcgc tggagcgagg cgtggcggtg gagagcgacg
     7081 ggccggcggg gaggcggctc gtggggtgcg gccggccggg cgaggaggtc cgcgtcgtca
     7141 atcccgagac ggcggtccct ctcggcgacg gcgccgtggg ggagatctgg gtcgccggac
     7201 cttccgtggc ggcggggtat tgggagcggc cgggcgcgag cgcggagacg ttcggagcgc
     7261 gtctggccgg agaggagggg ccgcccttcc tccgcaccgg cgacctcggg ttcctcgcgg
     7321 tcggcgagct gtatgtcacc ggacgcatca aggacctgat catcgtccgg ggccggaacc
     7381 tctatcccca ggacatcgag cggacggccg agggggcgca ccgggcgttg cggccggggg
     7441 gaggcgcggc cttctccgtg gaggtggacg gggaggagcg gctggtggtc gccgccgagg
     7501 tggagcggcg gtcggaatcg gaggcgccac gggcggcggc ggctgtccgc cgcgcggtgg
     7561 tggaggagca cgaggtccag gtccacgagg tggtgctgct cgccgctggc acgctccccc
     7621 ggacctccag cggcaaggtg cgccgtggcg cctgccgcgc cgcctacctg gcgggcggcg
     7681 gcctcgaggt ggtggggcgg agcgctccgg ccggttccct aggagaggtg gaggaggatc
     7741 tccccggcct gc
//
LOCUS       DASPJI010000849         2969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_855563, whole genome shotgun sequence.
ACCESSION   DASPJI010000849 DASPJI010000000
VERSION     DASPJI010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855563"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VEL74_23950"
     CDS             <1..340
                     /locus_tag="VEL74_23950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HYG65658.1"
                     /translation="GDVYDPVPPDERFDLIFWNMPFGFVEPDRQLTPLQRSNLDPGYA
                     SVRRYLTEAHRHLADGGLLTLGFSMDLGRLELIEELAAGAGLKVRIARSADAVPDRPY
                     DLRLLHLLPG"
     gene            complement(329..982)
                     /locus_tag="VEL74_23955"
     CDS             complement(329..982)
                     /locus_tag="VEL74_23955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG65659.1"
                     /translation="MSSGYFRSLDLVRRTFASAPLPTRLHTLGRYLTCPFLPVVGVLP
                     PGARVLDLGAGHGTFARLAVEQGASRVVAVEPDVRKILPTFRHPRIQFVGGYAEAVSG
                     AFDVVTVFDVLCRVPMAAWDGILRVALERLAPGGVILLKEIDPEHRLKGAWNRTQERI
                     ADLLGMTLGDAFSYETRDEMRRRLERLGFEGFEAVELGAGYPHAHILYRARRPALPR"
     gene            1034..2821
                     /locus_tag="VEL74_23960"
     CDS             1034..2821
                     /locus_tag="VEL74_23960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65660.1"
                     /translation="MHPQPARPAWRSPAYLLGLAAVAAARLLTLPASLWEWDEILFVK
                     GIEDYDPVHHQPHPPGYPLLIGLGKAFNLFLNDPFHAMVALSVVSSLIGYVALAAAFQ
                     RMAGEGPAAERIAVAGSVLFHLSPTLLVYGPLALSDSPALMFLSLALAAAARLPEGTT
                     AAALALGAAAAAAVGSRPQLCIAVLPLLAVALAFARGWRPRFLALAAFTLVSLTWFIP
                     LVVAVGGLGELLPFLGKQADLVVRYDKELPRSGNPPGWVATRFLAHPWGTRLTSLPVL
                     GLAAVGFAWLVKGRRWRALPLLVLSGIDLALGLAIMNPHDAVRYALPSLMAVSFAAAV
                     GGDALARLVRLPAAAYAMVALLVAGFVIYTRPLLEARATTPSPPMQAALWAERHARYD
                     AIFLVEDHLAAHASYLFEKFQRVPIGPGLERYAGEQVAPVYLLGDGESGWPGAVTFQW
                     PESDAYGKLSRGLYRVTSISPLLPGRRYQVVRGVYAYEPTAREAGWRWMDDDAAIRLF
                     PGGAARAELTLRLHPEVPWPANRVTVLVAGAPTATLEIPRGEERRVGVPLPAASRGAV
                     EIAFRSDRAFVPTDRRRLAVQLVNVERKP"
BASE COUNT          374 a         1109 c         1025 g          461 t
ORIGIN      
        1 cggtgacgtt tacgatccgg tccccccgga cgagcgtttc gacctgatct tctggaacat
       61 gcccttcggc ttcgtggaac cggaccggca gctcaccccg ctgcagaggt cgaacctcga
      121 tcccgggtac gcctcagtcc gccgctacct caccgaggcg caccgccatc tcgcggacgg
      181 cggcctgctc actctcggtt tcagcatgga cctcggccgc ctcgagctga tcgaggagct
      241 tgccgccgga gccggcctca aggtacggat cgcccggtcg gccgatgcgg tcccggaccg
      301 cccgtacgac ctccggttgc tccatctcct accggggtag ggccggccgc cgcgctctat
      361 agaggatgtg ggcgtgggga tagcccgcgc ccagctccac ggcctcgaag ccctcgaagc
      421 cgaggcgctc gaggcgccgg cgcatctcgt ccctcgtctc gtagctgaag gcgtccccca
      481 gcgtcatccc caggaggtcg gcgatccgct cctgggtgcg gttccaggcc cccttcaggc
      541 ggtgctcggg gtcgatctcc ttgagcagga tgacgccgcc gggggcgagc cgctccaggg
      601 ccacccggag gatgccgtcc caggccgcca tggggacccg gcagaggacg tcgaagacgg
      661 tcaccacgtc gaaggcgccg gagaccgcct cggcatagcc gccgacgaac tggatgcggg
      721 ggtgccggaa ggtgggcagg atcttgcgca cgtccggctc cacggcgacc accctggagg
      781 ccccctgctc caccgccagg cgcgcgaagg tgccgtgccc ggctcccagg tcgagcaccc
      841 gcgcgcccgg cggcagcacc cccaccaccg gcaggaaggg gcaggtgaga tagcgcccca
      901 gggtatggag gcgggtgggc agcggcgccg acgcgaacgt gcgccgcacg aggtcgaggg
      961 atcggaaata gccggagctc accggcagag gatatataat ccgcccgctc caaacgatcc
     1021 ctgacagccg acgatgcacc cgcaacccgc ccgcccggcg tggcgttcgc ccgcctacct
     1081 cctcggcctc gccgccgtgg ccgcggcgcg cctgctgacc ctgccggcca gcctctggga
     1141 gtgggacgag atccttttcg tcaaagggat cgaggactac gacccggtcc accaccagcc
     1201 tcatccccct ggctacccgc tcctcattgg cctggggaag gcgttcaacc tcttcctgaa
     1261 cgaccccttc cacgcgatgg tggcgctcag cgtggtgtcg tcgctcatcg gctacgtggc
     1321 cttggccgcc gccttccaga ggatggcggg cgagggaccg gcggcggagc ggatcgcggt
     1381 ggcgggctcc gtcctcttcc atctctcgcc gacgctgctg gtctacggtc ccctggccct
     1441 gtcggactcg ccggcgctga tgttcctctc cctcgccctc gccgccgcgg cccgcctgcc
     1501 cgaaggcacc acggccgctg cgctggcgct tggagcggcg gcggccgcgg cggtgggctc
     1561 ccggccgcag ctctgcatcg ccgtcctccc cctgctcgcc gtcgccctgg cattcgcccg
     1621 cggctggcgc ccgcggttcc tggcgctcgc cgccttcacc ctggtgtcgc ttacctggtt
     1681 catccccctc gtggtcgccg tgggcggcct cggggagctg ctgccgttcc tgggtaagca
     1741 ggccgacctg gtggtgcgct acgacaagga gctgccgcga tccggcaacc cgcccggctg
     1801 ggtggcgacg cgcttcctcg cccatccctg ggggacgcgg ctgacgtcgc tccccgtgct
     1861 cggcctggcg gcggtgggct tcgcctggct ggtgaagggc cggagatggc gcgccctgcc
     1921 cctcctggtc ctctccggca tcgacctggc gctcgggctc gccatcatga acccccacga
     1981 cgccgtgcgc tacgccctcc cctccctgat ggcggtctct ttcgccgcgg cggtaggcgg
     2041 cgacgcgctg gcgcggctcg tccgcctgcc ggcggcggcc tacgcgatgg tggcgctcct
     2101 cgtcgccggc ttcgtgatct acacccggcc gctcctcgaa gcccgggcca ccaccccatc
     2161 cccgccgatg caggcggcgc tctgggccga acgtcacgcc cgctacgacg ccatcttcct
     2221 ggtggaggac cacttggcgg cccacgcctc ctacctgttc gagaagttcc agcgggtccc
     2281 gatcggaccg ggcctggagc gctacgccgg cgagcaggtg gcgccggtct acctgctggg
     2341 ggacggcgag agcggctggc cgggggcggt gacgttccag tggccggaga gcgacgccta
     2401 tggcaaacta tctcgcggtc tttacagagt cacctccatc agtccgctcc tccctggccg
     2461 gcggtaccag gtcgtgcgcg gcgtctatgc gtacgagccg accgcccggg aggcggggtg
     2521 gcgctggatg gacgacgacg cggccatccg gctcttcccc ggcggcgcgg ccagggcgga
     2581 gctgacgcta cggcttcatc ccgaggtgcc ctggccggcg aaccgggtga cggtgctggt
     2641 ggcgggagcg cccacggcca cgctggagat cccgcggggg gaggagcgcc gggtgggggt
     2701 gcctctgccc gcggcctccc ggggggcggt ggagatcgcg ttccggtcgg accgggcgtt
     2761 cgtgcccacc gaccgtcgcc ggttggcggt gcagctcgtg aatgtcgaga gaaagccatg
     2821 aagcacgccc cagaatcgtc ccgctccgtc tcggtcgtca tccccatgtt caacgaggag
     2881 gagtcgatcg agcacgcggt cgcctgcgcg gcctccgccc tggcccccca cgtcgacgac
     2941 tacgagatcg tggtggtgga cgacgcctc
//
LOCUS       DASPJI010000850         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_733074, whole genome shotgun sequence.
ACCESSION   DASPJI010000850 DASPJI010000000
VERSION     DASPJI010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733074"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VEL74_23965"
     CDS             <1..527
                     /locus_tag="VEL74_23965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65661.1"
                     /translation="FNSPVAMLLGDKRNLALLSERADSPVFSEEERQILRDHLPWTRR
                     VESTETTFQGERVPLLDFISSHREDLVLKKGRSLAGQDVFIGRFTSQDEWDKVLGEVA
                     PQRDWIVQAFAESRPYLFQNDEYGCSPHDAVWGPFVFGKTYAGAILRLQPKRLLGIVN
                     LTRGATEGVLLEVV"
     gene            complement(548..1231)
                     /locus_tag="VEL74_23970"
     CDS             complement(548..1231)
                     /locus_tag="VEL74_23970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65662.1"
                     /translation="MAEPVSNFVTPAPRAATGGSDLARTVLRAAWLSIALGLLIELLI
                     VVSMMLGGAAGSLPRIVADSVQKVSWSTIVCLGIAFGTAAGKARSAVMGILGLISAPI
                     GFTVARTLHKGTLEALGVAGTAAGGASPLLIGGLKGLEYGILGMVIGWVGRQAWGGFG
                     AHAGAGASIGFIFGGAILGVMVRDAAMPLTTTDLIARGINELLFPVGCALVLYAAGRL
                     GQRLGVEPS"
     gene            complement(1252..>2535)
                     /locus_tag="VEL74_23975"
     CDS             complement(1252..>2535)
                     /locus_tag="VEL74_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003945154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HYG65663.1"
                     /translation="GEVNVVVLGINVGAQPYWNAEATMLMHTRGILIMTPEGAMVLTG
                     KQALDYSGGVSAEDNSGIGGYERIMGPNGQAQYFAHDLGEACRILMRHYEHAYVAPGE
                     RFPRPAPTRDPRDRDVCASPHGGGFPTVGEVFSDATNPGRKKPFDIRRIMAAVADEDH
                     PSLERWYGMRDAENSVVWDAHLGGHPVCLLGFESRPLPRLGYVPVYGPDHWTGGTLFP
                     LSSKKTARAINAASGNRPLVILANLSGFDGSPESMRQWQLEYGAEIGRAVVNFEGPIV
                     FCVISRYHGGAFVVFSNALHDNMRVVALEGAHASVIGGAPAAAVVFAREVDRRTRHDP
                     RVAELERAIADPERTDRAVLRARLEETLAAVRSEKLGEVAEEFDQVHSVQRSRDVGSV
                     HDIIPPGRLRPYIIEELERGMERELAALRDKIRPV"
BASE COUNT          377 a          895 c          900 g          363 t
ORIGIN      
        1 tcttcaacag cccggtggcc atgctcttgg gggacaagcg gaacttggcc ctgctgtcgg
       61 agcgggccga ctcgccggtg ttctccgaag aggagcggca gatcctgcgc gaccatctgc
      121 cctggacccg gcgggtcgag agcaccgaaa cgacgttcca gggggagcgg gtgccgctgc
      181 tcgacttcat cagctcccat cgcgaggatc tggtcctcaa gaaggggcgc tccctcgccg
      241 gccaggacgt cttcatcggc cgtttcacga gccaggacga atgggacaag gtcctgggcg
      301 aggtcgcccc gcaacgcgac tggatcgtgc aggcgttcgc ggagtcgcgg ccgtacctct
      361 tccagaacga cgagtacggc tgctcgccgc acgacgcggt ctggggaccc ttcgtgttcg
      421 gcaagaccta cgccggcgcc atcctccggc tccagcccaa gaggctcctg ggcatcgtca
      481 acctgacgcg gggagccacc gagggggtgc tcctggaagt ggtctgacct cctccgcggt
      541 ccgtgcttca ggagggctcg acgccgagcc gctggcccag gcggccggcg gcgtagagca
      601 ccagcgcgca gccgacgggg aagagcagct cgttgatgcc gcgcgcgatg agatcggtgg
      661 tggtcagcgg catggccgcg tcgcggacca tgacgccgag gatggcgccg ccgaagatga
      721 agccgatcga ggcgcccgcg ccggcgtggg cgccgaagcc gccccacgcc tgccggccca
      781 cccagccgat gaccatgccc aggattccgt actccagccc cttgaggccg ccgatcagca
      841 ggggagaggc gccgccggcg gcggtaccgg cgacgccaag ggcctcgaga gttcctttgt
      901 ggagcgtgcg tgccaccgtg aatccgatcg gcgccgagat caggccgagg attcccatga
      961 ccgctgagcg cgccttgccg gccgcggtgc cgaaggcgat ccccaggcag acgatggtcg
     1021 accaggagac cttctggacc gagtcggcga cgatcctggg caggcttccg gccgcgcctc
     1081 ccagcatcat ggagaccacg atcagcagct cgatgagcag cccgagcgcg atggacagcc
     1141 acgccgcgcg cagcaccgtg cgcgccaggt cgctgccgcc ggtggccgct cggggcgccg
     1201 gcgtgacgaa gttcgagacc ggttcggcca tggggcggtt ccctccgtag gtcagaccgg
     1261 gcggatctta tcccggagcg cggccagctc tctctccatg ccgcgctcca gctcctcgat
     1321 gatgtacggc cgcaggcggc cgggcgggat gatgtcgtgc accgagccca cgtcgcgcga
     1381 gcgctggacg ctgtgcacct ggtcgaattc ctcggccacc tcgcccagct tctcggagcg
     1441 gaccgccgcc agcgtctctt ccaggcgcgc gcgcagcacc gccctgtccg tgcgctccgg
     1501 gtcggcgatg gccctctcca gctccgccac ccgcggatcg tgccgcgtcc gccggtccac
     1561 ctcccgggcg aagaccacgg ccgccgccgg cgcgccgccg atcaccgacg cgtgggcgcc
     1621 ctccagggcc accacccgca tgttgtcgtg cagcgcgttc gagaagacca cgaaggcgcc
     1681 gccgtggtac cgcgagatga cacagaagac gatcgggccc tcgaagttga ccaccgcgcg
     1741 gccgatctcg gcgccgtact cgagctgcca ctgccgcatg gactccgggg agccgtcgaa
     1801 ccccgagagg ttggcgagga tcaccagcgg ccggttgccg gaggcggcgt tgatcgcccg
     1861 cgccgtcttc ttcgacgaga gcgggaagag cgtgccgccc gtccagtggt ccgggccgta
     1921 gaccggcacg tagcccaggc gcggcagcgg ccgggactcg aatcccagca ggcagacggg
     1981 gtggccgccc aggtgggcgt cccatacgac gctgttctcg gcgtcccgca tgccgtacca
     2041 gcgctccagc gacgggtggt cctcatcggc caccgccgcc atgatccggc ggatgtcgaa
     2101 cggcttcttc ctccccgggt tggtggcgtc cgagaagacc tcgcccacgg tggggaagcc
     2161 gccgccatgg ggggaggcgc agacgtcgcg gtcgcgcggg tcgcgggtcg gcgccggccg
     2221 ggggaagcgc tcgccgggtg ccacgtaggc gtgctcgtag tggcgcatca ggatgcggca
     2281 ggcctcgccg aggtcgtggg cgaagtactg cgcctggccg ttggggccca tgatccgctc
     2341 gtagccgccg atgccggagt tgtcctcggc cgagacgccg cccgaatagt ccagggcctg
     2401 cttgccggtg agcaccatcg ccccctccgg agtcatgatg aggatgcccc gggtgtgcat
     2461 cagcatggtg gcctcggcgt tccaatacgg ctgggcgccg acgttgatgc cgaggacgac
     2521 gacgttcacc tcgcc
//
LOCUS       DASPJI010000851         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_226834, whole genome shotgun sequence.
ACCESSION   DASPJI010000851 DASPJI010000000
VERSION     DASPJI010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226834"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2169
                     /locus_tag="VEL74_23980"
     CDS             <1..>2169
                     /locus_tag="VEL74_23980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HYG65664.1"
                     /translation="DAETAEAADEGEEDSGGGVTSRRRRRRRRGEVDMELVGGSDDDP
                     PNTVTRVRAPRVAEAATSDEVVAVKGSTRLEAKKQRRRESRQSGRRRQVITEAEFLAR
                     RESVERQMLVRQKENRIQIGVLEDGVLAEHFVSHTQQDSLIGNVYVGKVQNVLPSMEA
                     AFIDIGRGRNAVLYAGEVNWDASRLEGGPRRIENALKSGDSVLVQVTKDPVGHKGARL
                     TSQISLPGRYLVYVPGGSMTGISRKLPDVERARLKKILKDRLPDGAGVIVRTAAEGAS
                     EQELTHDINRLRAQWEQIQERAGSTKTLAPEMLYAEPDLTIKTVRDVFNEDFTTMVVQ
                     GEEAWDNIEAYVTYVAPDLLDRLQKWDEDEDLFVNYRVEEQIQKALERKVYLPSGGSL
                     VIDRTEAMTVVDVNTGKFTGSGGNLEETVTKNNLEAAEEIVRQLRLRDIGGIIVIDFI
                     DMVLESNRDLVLRRLVECLGRDRTKHQVAEVTSLGLVQMTRKRMGTGLLEVFSEPCEH
                     CAGRGVVVHDHPLEGRSGGATGEFRDQRQNRTERKRSRRGDKAPEQPVQAEAPAREPD
                     PAVRAKAEAARTAFATIAAATHHETPAEDGRRPAAETAADAEAESRAEGSGRSRKKRR
                     GKRSRNRGQGAEAEQRQDEQTRPAESSGAPTERPAPAEQGSRLTINGEVIELPHGHAP
                     AAEEPAAAPLSLDSLVEALEQQAPAEPEAPSAPEAPAEPEA"
BASE COUNT          356 a          802 c          792 g          219 t
ORIGIN      
        1 ggacgccgag accgccgagg ccgccgacga gggcgaggag gactccggcg gcggcgtgac
       61 cagccggcgc cggcgccgcc gccgccgcgg cgaggtcgac atggagctcg tgggcggcag
      121 cgacgacgac ccgcccaaca ccgtcacccg cgtgcgcgcg ccccgcgtcg cggaggccgc
      181 cacgagcgac gaggtcgtcg ccgtgaaggg ctccacgcgc ctcgaggcca agaagcagcg
      241 ccgccgcgag tcccgccagt ccggccgccg ccgccaggtg atcacggagg ccgagttcct
      301 cgcccgccgc gagtcggtcg agcggcagat gctggtccgg cagaaggaga accggatcca
      361 gatcggcgtc ctcgaggacg gcgtgctcgc cgagcacttc gtctcgcaca cccagcagga
      421 ctcgctgatc ggcaacgtct acgtgggcaa ggtgcagaac gtgctgccgt ccatggaggc
      481 cgcgttcatc gacatcggcc gcggccgcaa cgccgtgctc tacgccggtg aggtcaactg
      541 ggacgcgtcc cgcctcgagg gcgggccccg ccggatcgag aacgccctga agtccgggga
      601 ctccgtcctc gtgcaggtca ccaaggaccc ggtcgggcac aagggcgccc gcctcaccag
      661 ccagatctcc ctgcccggcc gctacctcgt ctacgtcccc ggcgggtcca tgaccggcat
      721 ctcccggaag ctgcccgacg tcgagcgcgc ccggctgaag aagatcctca aggaccggct
      781 gccggacggc gcgggcgtga tcgtgcgcac ggccgccgag ggcgcctccg agcaggagct
      841 gacccacgac atcaaccggc tgcgcgccca gtgggagcag atccaggagc gcgccgggtc
      901 cacgaagacg ctggccccgg agatgctcta cgccgagccg gacctcacca tcaagaccgt
      961 ccgcgacgtc ttcaacgagg acttcaccac gatggtggtc cagggcgagg aggcgtggga
     1021 caacatcgag gcctacgtga cctacgtggc cccggacctg ctcgaccggc tccagaagtg
     1081 ggacgaggac gaggacctgt tcgtcaacta ccgtgtggag gaacagatcc agaaggcact
     1141 cgagcgcaag gtctacctcc cgtcgggcgg ttcgctggtg atcgaccgga ccgaggccat
     1201 gacggtcgtc gacgtcaaca ccgggaagtt cacgggctcc gggggcaacc tcgaggagac
     1261 cgtcaccaag aacaacctgg aggccgccga ggagatcgtg cggcagctgc gcctgcggga
     1321 catcggcggg atcatcgtca tcgacttcat cgacatggtc ctggagtcca accgcgacct
     1381 cgtgctccgc cggctcgtgg agtgcctggg ccgggaccgc acgaagcacc aggtggcgga
     1441 ggtgacctcg ctgggcctcg tgcagatgac ccgcaagcgc atgggcaccg gcctgctcga
     1501 ggtgttctcc gagccgtgcg agcactgcgc cggccgcggc gtcgtcgtcc acgaccaccc
     1561 gctggagggc cgctccggcg gggccaccgg ggagttccgc gaccagcggc agaaccgcac
     1621 ggagcgcaag cgcagccgcc ggggcgacaa ggccccggag cagccggtcc aggccgaggc
     1681 acccgcccgc gagccggacc ccgcggtgag ggccaaggcc gaggccgcgc ggaccgcgtt
     1741 cgccaccatc gccgcggcca cccaccacga gaccccggcc gaggacggcc ggcggccggc
     1801 cgcggagacc gccgcggacg ccgaggcgga gtcccgggcg gagggctccg ggcggtcccg
     1861 gaagaagcgg cgcggcaagc gcagccgcaa ccggggccag ggcgccgagg ccgagcagcg
     1921 ccaggacgag cagacccggc ccgccgagtc ctccggggcc cccacggagc gcccggcgcc
     1981 cgccgagcag ggctcccgcc tgacgatcaa cggcgaggtg atcgagctgc cgcacggcca
     2041 cgcgccggcg gcggaggagc ccgccgcggc gccgctgagc ctcgactccc tcgtggaggc
     2101 cctggagcag caggcgcccg ccgagccgga ggccccgtcc gcgccggagg cgccggccga
     2161 gccggaggc
//
LOCUS       DASPJI010000852         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_226880, whole genome shotgun sequence.
ACCESSION   DASPJI010000852 DASPJI010000000
VERSION     DASPJI010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226880"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VEL74_23985"
     CDS             complement(<1..391)
                     /locus_tag="VEL74_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65665.1"
                     /translation="MPRRGGAFAAVVVSTLLGSGLGAQVEIDSHTFGGLEARALGPAV
                     MSGRVSAVAAVPGDPLTIYVGAASGGVWKSGDGGVTWKPVFDGHAQSIGAIAVDPANP
                     ANLWVGTGESWVRNSVSVGDGVYRSTDA"
     gene            complement(397..1200)
                     /locus_tag="VEL74_23990"
     CDS             complement(397..1200)
                     /locus_tag="VEL74_23990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65666.1"
                     /translation="MIRKAFPCLLAISLATMAVMHPSVLGAQAAAPTAAAPTVDELVE
                     KNLQAKGGKEKLQALKGLRMTGKMAMGPGMEGSLLIETARPDKMRSDVTIQGMTVSQA
                     YDGKTGWKVMPFTGKTDAEPMTEEELKQMQRQATGMDGLMGYKERGETLEYLGKADLE
                     GTPVHKLKWTRQGGDSAVLYLDAEHFLELRADGKALVQGQQMESVTTFGDYKEVGGIL
                     FAHSMQLSMPGGMGGMTMTIDKIEVNPDLPASRFAMPAKAEAKMPAPKG"
     gene            1493..>2000
                     /locus_tag="VEL74_23995"
     CDS             1493..>2000
                     /locus_tag="VEL74_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65667.1"
                     /translation="MRRRISLLTSAALAVSLAVPVASLAANPGTIDHGLLAGMAARSI
                     GPAAMSGRIAAIEASVEDPTLVYVGTASGGVWKSVNGGLTWEPVFDDQPVTSIGSLAL
                     APGNPDVVWVGSGEGNPRNSVSVGEGVFRSLDGGRTWRRAGLEKTERIHRLLLDPRDP
                     DVAYSAAMG"
BASE COUNT          275 a          743 c          672 g          310 t
ORIGIN      
        1 cggcgtcggt gctgcggtag acgccgtcgc ccaccgagac gctgttgcgc acccacgact
       61 cgccggtccc cacccagagg ttcgcggggt tcgcgggatc gaccgcgatg gcgccgatgg
      121 actgggcgtg gccgtcgaac accggcttcc aggtgacgcc gccgtcgccc gacttccaga
      181 cgccgccgct cgccgcgccg acgtaaatgg tgagcggatc gccggggacc gccgcgacgg
      241 ccgagacccg gccgctcatc accgccggcc ccagcgcccg cgcctccagg ccgccgaagg
      301 tatgggaatc gatctccacc tgagccccca gcccggagcc cagcagagtg ctcacgacga
      361 cggcggcgaa ggctccccct ctacgcggca tagggcctac cctttcggcg ccggcatctt
      421 ggcctccgcc ttggccggca tggcgaagcg cgacgcgggg agatcggggt tgacctcgat
      481 cttgtcgatg gtcatggtca tgccccccat gccgccgggc atggagagct gcatggagtg
      541 ggcgaagagg atgccgccca cctctttata gtcgccgaag gtagtgacgc tctccatctg
      601 ctggccctgg accagggctt tgccgtccgc ccgcagctcc aggaagtgct ccgcgtccag
      661 atagaggacg gccgagtcgc ccccctggcg ggtccacttc agcttgtgga cgggcgtccc
      721 ctccaggtcc gccttgccga ggtactccag cgtctccccg cgctccttgt agcccatcag
      781 gccgtccatg cccgtggcct ggcgctgcat ctgcttgagc tcctcctcgg tcatcggctc
      841 ggcgtccgtc ttgccggtga acggcatcac cttccagccg gtcttgccgt cgtaggcctg
      901 ggagacggtc atcccctgga tggtgacgtc ggaacgcatc ttgtccggac gggcggtctc
      961 gatgaggaga gagccctcca tccccggccc catggccatc ttgccggtca tgcgcaggcc
     1021 cttgagcgcc tggagcttct ccttgccacc cttggcctgg aggttcttct ccaccagctc
     1081 gtcgacggtc ggggcggcgg ccgtcggggc ggcggcctgg gcgccgagga cggacggatg
     1141 catcacggcc atcgtggcca gggagatcgc cagcagacag ggaaacgcct tgcggatcat
     1201 cggttcggtc tcctacgaga tgggacgttc gcgcgggagg atagccggag gcggcccctg
     1261 ttgaccagcc ggtggcagcc ggcccccagg cgcggcgaag ggcctggggg gaaacgtagg
     1321 gagtcgcagc actgccacgt ccccgcccca ggccctccgc ggcgccgagg aggcgcccat
     1381 cccatatacg gctccggcgc ccgaatgttt catgggggcg gcgagttgct ccgccgcgcc
     1441 gccccggcta ctcttgcccg gtttccatcc gattcaactc tcggaggaca gaatgcgcag
     1501 gagaatctct ttgctcacct cggcggccct ggccgtgagc ctggccgtgc cggtggccag
     1561 cctggccgcc aacccgggga ccatcgacca cggcctgctc gccggcatgg cggcccgctc
     1621 catcggcccg gcggcgatga gcggccgcat cgccgccatc gaagcctcgg tggaggaccc
     1681 caccctggtc tacgtgggga ccgccagcgg cggcgtctgg aaatcggtca acggcggcct
     1741 cacctgggag ccggtcttcg acgaccagcc ggtgacctcc atcggctccc tcgccctcgc
     1801 gcccggcaac cccgacgtcg tctgggtggg cagcggcgag ggcaaccccc gcaacagcgt
     1861 ctcggtgggc gaaggggtct tccggtcgct cgacggcggc cgcacctggc gccgcgccgg
     1921 cctcgagaag accgagcgca tccaccgcct gctcctcgac ccgcgcgacc ccgacgtcgc
     1981 ctactccgcc gccatgggcc
//
LOCUS       DASPJI010000853         8806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_799039, whole genome shotgun sequence.
ACCESSION   DASPJI010000853 DASPJI010000000
VERSION     DASPJI010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8806)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8806
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799039"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="VEL74_24000"
     CDS             complement(<1..1365)
                     /locus_tag="VEL74_24000"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HYG65668.1"
                     /translation="MRDPQSPSQDVDDLAELARRLSERGRYEEAAELFMLALRLDPKN
                     LSVKLGLAEARKLQTQQRGGTSRSLKEMMRESFRRNAIDAAHFLGLAHLYAEKGENAR
                     AIECLEIAKAKDLANPSHHKLHGRLLFRRKDYDGAAEEFARALRLNPFDRETAESLGR
                     TEYDRKQFEPALRASVHAFLLIHEADVESVERLRRRIQTLKQILGWGNRELAAAFRER
                     KEHLHTAFDRLEWHRERFLEQGGLTVSAILPSPPSRRTEGGSRIEQAARLRRIRAWTH
                     LTDEQVFRLTQTVEEEVHDTGSLVLGYRSQGRDLYVLERGEIAVQRSTSYGTFGLGRL
                     QAGDVFGESSFITGHERSSDAVATKPCQILRIDAERLDQVVESYPDLGVQLYWSLWHS
                     LAHKLRNTNEQLKTFFSDEALPENFLRLRKQPSAGAAPVKVEANDKIRLFREQGLSRR
                     ELMTL"
     gene            1538..2017
                     /locus_tag="VEL74_24005"
     CDS             1538..2017
                     /locus_tag="VEL74_24005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65669.1"
                     /translation="MNAFREDVAIALHRGIECCRREEWDQGLLYLGQITRSDERNGLP
                     GLFYSYLGYGIARCQRRFEEGRKLCEHAIKVEFYQPENYLNLARTCLLTSDRDAAVRA
                     IRRGLKIDPHNLELLSLQRQVGVRGAPVLPFLSRSNLVNRILGRIRHAMQGRPPGVK"
     gene            complement(2076..2528)
                     /locus_tag="VEL74_24010"
     CDS             complement(2076..2528)
                     /locus_tag="VEL74_24010"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HYG65670.1"
                     /translation="MAMNVSGAGMGPRSDINITPLVDVVLVLLIIFMVITPLLQMGYE
                     VQVPPKVEGQIAPSAMDDQVIVRMDAEGRPFINKQQVPLAEFPSRLQEVLAGRASKVV
                     FFAADGELPYDQVADFMDMCRDNGAENLGIVFDDLRSGGGAAPATAGP"
     gene            complement(2556..2999)
                     /gene="tolR"
                     /locus_tag="VEL74_24015"
     CDS             complement(2556..2999)
                     /gene="tolR"
                     /locus_tag="VEL74_24015"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HYG65671.1"
                     /translation="MLPKKKPSPFADVGVKSEINVTPLVDVCLVMLIIFMVVTPMLQK
                     GVDVALPETSAPEKMPENEKQLTVSIKQDGNVFVGQNWIPNENLEAQLADIYSQTPDK
                     QIVVKGDRRLKYKDVRKVMQLLNEAGFSRVGLVTNKEDRSGGGHA"
     gene            complement(2989..3672)
                     /locus_tag="VEL74_24020"
     CDS             complement(2989..3672)
                     /locus_tag="VEL74_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HYG65672.1"
                     /translation="MDFSPVALWEQMTILNKAVIVILIVMSVWSLYVCVDRILVFRKA
                     KKQSLAFAKLATEHLKNDRPQAAIDAAAKYPGSHLARVVSAGLQSFQHENQHSPLSDI
                     EVVEAASRAVERATLMTTSDFKRGIGGLATIATTTPFIGLFGTVIGIINAFRGMAMTG
                     SGGIGAVSAGIAEALVATALGLGVAIPAAWMFNYFTGKIERLQVEMSNSASELIDFFM
                     KKRKVRNAA"
     gene            complement(3704..4495)
                     /locus_tag="VEL74_24025"
     CDS             complement(3704..4495)
                     /locus_tag="VEL74_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HYG65673.1"
                     /translation="MFEDSLLDISEDKTRAKRRARGILIMQIALIIHLVAGAAVLLAN
                     YWQVNPVPEPPINVVFFSAAAPPPPPPPPPPPPPAAKTQPKVEQKVVEVIRPTEPVQP
                     VEVPEEIPETSEEPVEAVEGGVEGGVEGGVVGGVVGGVVGGVVGGVVGGVVGGVPGGV
                     PGGVVTDEPLRVGGGVSKPEKISGPPPVYTELARKARLQGTVIVEAIIDERGNVTNVR
                     VLKGLPMGLDRAAVEAVQKWKFRPAQFQGKPVKVYYSLTVNFQVQ"
     gene            complement(4500..5342)
                     /locus_tag="VEL74_24030"
     CDS             complement(4500..5342)
                     /locus_tag="VEL74_24030"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG65674.1"
                     /translation="MQRPLVVPALLLLLLPTVGCNKIRARAELKNGNALYANEQYKGA
                     LTQFQKGLELDPEATFAWRSVGLSALALYKPGDTSPQNVQYAKTATDAFEKYLRDHPD
                     DEKIREYLLSTYVNAKMYPEAMAYLDEQAKAHPNDPQYLNAKVRILILSGQFQEAERM
                     AAQYTGPNRHEIFYAIGHGAYEKVYNDPDLNVEQRTALADTGLEALQRSIEAKPDYFQ
                     SLVYYNLMLREKAKMETDANKRMEYLAEADQWRDKAVAQGKKDNESKKAAEAKQSEPK
                     KVEG"
     gene            complement(5568..6851)
                     /locus_tag="VEL74_24035"
     CDS             complement(5568..6851)
                     /locus_tag="VEL74_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HYG65675.1"
                     /translation="MRLPLHHKILIGGLLGAGAGVAAHYLFGADNPSLDGFIRYVTGP
                     IGQIFLRLLFMLVVPLIFSALVLGVAGIGDPRSLGRVGVKTLAYTVLVSAIAVLIGVT
                     LVNVLRPGEGISPELRARLTEGAAERASAVAGGEAPKTGVDLFLDMVPRNPVKAAADG
                     DMLGVMFFSLMIGLGLALTPTLATERFREAVEGLYDITMRLIGIVISLAPIGVAALLF
                     NLTAELGYEILQQLAVYVGVVVLALAIHQFIVYSLAVRFLGGMSPRTFFRGIQAAMIT
                     AFSTASSNATLPTALRVAEENLRLPPSVSRFVLTIGSTMNQNGTALFEGVTVLFLAQF
                     YGVELSLGQQVTVVLICILGGIGTAGVPAGSIPVVALILRQVGVPVEGIGLILGVDRF
                     LDMCRTTLNVTGDLAAAVVVSRGEAEAPAAADSSS"
     gene            6987..>8806
                     /locus_tag="VEL74_24040"
     CDS             6987..>8806
                     /locus_tag="VEL74_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HYG65676.1"
                     /translation="MADFRGFRIVHTRARRGRLFPVWGFPLLRGIFKTKSLNAILASA
                     EAPHTALNRSLGTLQLTLLGIGAIIGAGIFSTVGTAAAGGASHLGAGPGLVLSFVITA
                     ITCGFAALCYAEFASMVPVSGSAYTYSYATLGELVGWIIGWDLIIEYAVGNIAVAISW
                     SGYFQELLRGFGLNWPGWLGTDYRTALQAAGQVAAARAEGTDPAALGLAVERGAQALA
                     EAPRLFGMPLVFNVPAFLIVMLITVVLVVGIRESAWFNSSMVVLKLAIIAFFIGLGAF
                     YVQPENWTPFAPNGFVGIAGAAAIIFFAYIGFDAVSTAAEETKDPGRSMPIAIIASLA
                     VCTLIYIAVALVLTGMLPWTQLGTAEPLATAFSALGMGWPALIISLGAVFATTSVLVV
                     FQLGQPRIFFSMARDGLLPSWAARVHPRYRTPHITTWITGIVVAFFAGVANINEVVEL
                     TNIGTLFAFLLVCVGITVLRYKDPDRPRRFRVPLGPWIIPMLGAASCIFLMVYLPPAS
                     WWRFIGWLVLGMAVYFAYGYSHSTVGREAGRSPRPRLPEKLAAAGFLSAGIGLFTIPH
                     DAGLPRLLQEAATAGDPDHDRALVGLMLIAAGLVLVVIGAL"
BASE COUNT         1289 a         3089 c         2900 g         1528 t
ORIGIN      
        1 gagggtcatc agctcccggc gcgacaggcc ctgctcgcgg aacagccgga tcttgtcgtt
       61 ggcctccacc ttgaccggcg ccgccccagc cgacggctgc ttgcgcaggc gcaggaagtt
      121 ctccggcagc gcctcgtcgg agaagaaggt cttgagctgc tcgttggtgt tgcggagctt
      181 gtgggcgagg ctgtgccaga ggctccagta gagctggacg cccaggtcgg ggtaggactc
      241 caccacctgg tcgaggcgct cggcatcgat gcggaggatc tggcagggct tggtggccac
      301 ggcgtcggac gagcgctcgt ggccggtgat gaagctcgac tcgccgaaga cgtcccccgc
      361 ctgcagccgg ccgaggccga aggtgccgta ggacgtgctg cgctggacgg cgatctcgcc
      421 gcgctccagg acgtagaggt cgcgcccctg gctgcgatac cccaggacga ggctgccggt
      481 gtcgtggacc tcctcctcca ccgtctgggt gagccggaag acctgctcgt cggtgagatg
      541 ggtccaggcc cggatccgcc gcagccgcgc cgcctgctcg atgcggctgc ccccctcggt
      601 ccgccgggag ggcggagagg gaaggatggc gctgacggtg agccccccct gctccaggaa
      661 ccgctcgcgg tgccactcca gccggtcgaa cgccgtgtgc aggtgctcct tgcgctcccg
      721 gaaggccgcg gcgagctcgc ggttgcccca gcccaggatc tgcttgagcg tctggatgcg
      781 ccgccgcagc cgctcgacgc tctcgacgtc ggcttcgtgg atcagcagga aggcgtggac
      841 gctggcacgt agcgccggct cgaactgctt gcggtcgtac tcggtgcggc cgaggctctc
      901 ggccgtctcc cggtcgaagg ggttgaggcg cagggcgcgc gcgaactcct cggcggcgcc
      961 gtcgtagtcc ttgcgccgga agagcagccg gccgtgcagc ttgtggtgcg acgggttcgc
     1021 cagatccttg gccttggcga tctccaggca ctcgatcgcc cgcgcgttct cccccttctc
     1081 ggcgtagaga tgggcgagac ccaggaagtg ggctgcgtcg atggcgttgc ggcggaagct
     1141 ctcccgcatc atctccttca ggctgcggga ggtcccgccg cgctgctggg tctggagctt
     1201 gcgcgcctcc gccagcccga gcttgacgct caggttcttg gggtcgagac gcaacgcgag
     1261 catgaagagc tcggctgcct cctcgtagcg gcctcgctcc gagagacgcc tcgcgagctc
     1321 ggccagatcg tccacgtcct gcgacggact ctgcgggtct ctcatcgtcg gtgctgtccc
     1381 cggagagtgc ccagtttagc acgggcggtg ccctgcggct gccgggcggc agtgggggag
     1441 acttgaaaaa ttcacgcggc ctatctatat ttctctatat tgtagaccct tttccgaatc
     1501 ggcccgagcc ggtcctggac tctgcggagg ccctgtggtg aacgctttcc gtgaagatgt
     1561 ggccatcgcc ctccaccgcg gcatcgagtg ctgccgccgg gaggaatggg atcaggggct
     1621 gctctacctg ggccagatca cccggtcgga cgagcgcaac gggctccccg gcctcttcta
     1681 ctcctatctg gggtatggga tcgcccgctg ccagcgccgg ttcgaggagg gccgcaagct
     1741 ctgcgagcac gcgatcaagg tagagtttta ccagccggag aactacctga acctggcgcg
     1801 cacctgcctg ctcaccagcg accgggacgc cgccgtccgt gccatccgcc gagggctgaa
     1861 gatcgacccc cacaacctgg agctgctctc cctgcagcgc caggtcggcg tgcgcggggc
     1921 gccggtgctc cccttcctgt cgcgttcgaa cctggtgaac cggatcctgg ggcggatccg
     1981 ccacgccatg caggggcggc cgcccggggt gaaatagaaa cggcggccgg ttgcccggcc
     2041 gccgctcgcg gtcaattcca gatgcagtcc ggggctcagg ggcccgcggt cgccggcgcc
     2101 gcaccgccgc cgctccgcag gtcgtcgaag acgatcccga ggttctcggc gccgttgtcc
     2161 cggcacatat ccatgaagtc cgctacctgg tcgtagggca gctcgccgtc ggcggcgaag
     2221 aagaccacct tcgacgcgcg tccggcgagc acctcctgga gcctgctcgg gaactcggcc
     2281 aacgggacct gctgcttgtt gatgaacggg cggccctcgg cgtccatgcg cacgatcacc
     2341 tgatcgtcca tcgccgatgg agcgatctgc ccctccacct tgggcggtac ctgcacttca
     2401 tagcccatct ggaggagcgg cgtgatcacc atgaagatga tcagcagcac cagcaccacg
     2461 tcgacgagcg gcgtgatgtt gatgtcgctc cgcgggccca tcccggcgcc ggatacgttc
     2521 attgccatgg tcgttacctc cgtcggtacc cggcgtcagg cgtgaccgcc gccgctccga
     2581 tcctccttgt tggtcaccag gcctacccgg ctgaacccgg cttcgttgag gagctgcatc
     2641 accttacgaa cgtccttgta cttgagccgg cgatccccct tgaccacgat ctgcttgtcg
     2701 ggcgtctggg agtagatatc ggccagctgc gcctcgaggt tctcgttggg gatccagttc
     2761 tggcccacga agacgttgcc gtcctgcttg atggacacgg tgagctgctt ctcgttctcc
     2821 ggcatcttct cgggtgccga cgtctccggc aaggccacgt ccacgccttt ctggagcatg
     2881 ggcgtgacca ccatgaagat gatcagcatc acgaggcaca cgtcgacgag gggcgttacg
     2941 ttgatctcgc tcttgacccc gacgtcggcg aacgggctgg gcttcttctt aggcagcatt
     3001 acggaccttc cgcttcttca tgaaaaagtc gatgagctcg gaggcggagt tggacatctc
     3061 gacctgcagg cgctcgatct tgccggtgaa gtagttgaac atccaggccg ccgggatggc
     3121 gacgccgagg ccgagcgcgg tggcgacgag cgcttcggcg atacccgcgg agaccgcgcc
     3181 gataccaccc gagccggtca tggccatgcc gcggaacgcg ttgatgatgc cgatgacggt
     3241 tccgaacagg ccgatgaacg gcgtggtggt ggcgatcgtg gccaggccgc cgatgccgcg
     3301 cttgaagtcg gaggtggtca tcagggtggc gcgctcgacg gcgcggctgg cggcttccac
     3361 gacctcgatg tccgacagcg gactgtgctg gttctcgtgc tggaaggact ggaggcccgc
     3421 ggagacgacc cgggcgaggt ggctgccggg gtacttggcc gccgcgtcga tcgccgcctg
     3481 cgggcggtcg ttcttgaggt gctcggtcgc cagcttggcg aacgccagcg actgcttctt
     3541 cgccttgcgg aagacgagga tgcggtcgac gcagacgtag agagaccaga ccgacatgac
     3601 gatcaggatg acgatgaccg ccttgttgag gatcgtcatc tgctcccaga gggctaccgg
     3661 actaaaatcc atgctcttct cctttgctct ttcagcgcag cttctactgc acctggaaat
     3721 tgaccgtcag cgagtaatag accttgaccg gcttgccctg gaactgggca ggcctgaact
     3781 tccacttctg gaccgcttcg acggcggccc ggtcgaggcc catgggcagc cccttgagca
     3841 cccgcacgtt ggtgacgttg ccgcgctcgt cgatgatcgc ctcgacgatg accgtgccct
     3901 ggagacgggc cttgcgcgcc agctcggtat agaccggcgg gggtcccgag atcttctcgg
     3961 gcttgctgac gccgccgccg acccgcagcg gctcgtcggt caccacgccg ccggggacgc
     4021 cgcccgggac gccaccgacc acaccgccga cgacgccgcc gacgacaccg cccaccacac
     4081 caccgacgac accaccgacc acgccgcctt cgacaccgcc ctcgacgccg ccttcgacgg
     4141 cctcgaccgg ctcttcagac gtctcaggga tctcctccgg cacctcgacc ggctgcaccg
     4201 gctcggtggg acggatgacc tccaccacct tctgctccac cttgggctgc gtcttggcgg
     4261 ccggcggggg gggcggcggc ggcggcggcg ggggcggggg cgccgcggcg ctgaagaaca
     4321 cgacgttaat cggcggctcc ggcaccggat tcacctgcca gtagttcgcc agcaggacgg
     4381 cggcgccggc gaccaggtgg atgatcaggg cgatctgcat gatcaggatg ccccgcgccc
     4441 gccgcttggc cctcgtcttg tcctcgctga tatcgagtag agaatcttcg aacatcgctt
     4501 cacccctcaa ccttcttggg ttcggactgc ttggcttcgg ccgctttctt ggactcgttg
     4561 tccttcttcc cctgcgccac cgccttgtcc cgccactggt ccgcctcggc caggtactcc
     4621 atccgcttgt tggcgtcggt ctccatcttg gccttctcgc ggagcatgag gttgtagtag
     4681 accagcgact ggaagtagtc gggcttggcc tcgatggacc gctgcagcgc ttcgaggccg
     4741 gtgtcggcca gcgccgtccg ctgctcgacg ttgaggtccg gatcgttgta gaccttctca
     4801 tacgctccgt ggccgatcgc gtagaagatc tcgtggcggt tggggccggt gtactgggcc
     4861 gccatccgct ccgcctcctg gaactgcccc gagaggatga ggattctcac cttcgcgttc
     4921 aggtactgcg gatcgttggg gtgggccttc gcctgctcgt ccaggtaggc catcgcctcc
     4981 ggatacattt tggcgttgac gtaggtcgac agcaggtact cccggatctt ctcgtcgtcc
     5041 ggatggtcgc ggaggtactt ctcaaaggca tcggtggccg tcttggcata ctgcacgttc
     5101 tgcgggctcg tgtctcccgg cttgtagagg gccagcgccg agagtccgac ggagcgccag
     5161 gcaaaggtcg cctccgggtc cagctccagg cccttctgga actgggtgag agcccccttg
     5221 tactgctcgt tggcgtacag ggcattcccg ttcttgagct ccgcccgcgc cctgatcttg
     5281 ttgcagccca cggtgggcag cagcaagaga agaagggccg gtactaccaa aggtctttgc
     5341 atagtcacct ctcccagcct tagacagggg gagccgccaa ttagttccga gaggggaggc
     5401 gcaccgaatt cgctctgcca cgccccttcc ggggccccct ccctcggtcg gtcacgccct
     5461 agaaatcagg gccgagggat ctggcaggag gagaagaatt tccctccccc ccgaccaggc
     5521 tgaagcacga aacatgccgt ttcccggaag cgattcccga ccggccttca agacgaagaa
     5581 tcggcggcgg ccggcgcttc ggcctcgccc cgcgacacca ccaccgccgc cgccaggtcg
     5641 ccggtgacgt tgagcgtggt gcggcacatg tcgaggaacc ggtcgacccc caggatcagc
     5701 ccgatgccct ccaccggcac ccccacctgg cgcaggatca gcgccaccac cgggatcgag
     5761 ccggcgggaa cgcccgccgt gccgatgccg cccaggatgc agatcagcac caccgtcacc
     5821 tgctggccga gcgacagctc caccccatag aactgcgcca ggaagaggac cgtcaccccc
     5881 tcgaagaggg cggtcccgtt ctggttcatc gtcgagccga tggtcagcac gaaccggctg
     5941 acgctcggcg gcagccgcag gttctcctcc gccacccgta gcgccgtcgg cagggtggcg
     6001 ttgctcgagg cggtggagaa ggcggtgatc atcgccgcct ggatgcctct gaagaaggtg
     6061 cgggggctca tcccccccag gaagcgcacc gccagcgagt agacgatgaa ctggtggatc
     6121 gccagggcga gcaccaccac ccctacgtag accgcgagct gctgcaggat ctcgtagccc
     6181 agctcggcgg tcaggttgaa cagcagcgcc gcgacgccga tgggggcgag cgagatcacg
     6241 atgccgatga ggcgcatggt gatgtcgtag agcccctcca ccgcctcccg gaaccgctcc
     6301 gtcgccaggg tgggggtgag cgcgaggccc aagccgatca tcagggagaa gaacatcacc
     6361 cccagcatgt cgccgtcggc cgccgccttg accgggttcc ggggcaccat gtcgaggaac
     6421 aggtcgaccc cggtcttcgg cgcctccccc ccggccaccg ccgacgcccg ctcggccgcc
     6481 ccctcggtca gccgcgccct gagctcgggg gagatcccct ctcccggccg caggacgttg
     6541 accagggtga cgccgatgag gaccgcgatg gccgacacca gcaccgtgta cgccagcgtc
     6601 ttgacgccga cccggcccag ggaccggggg tccccgatgc ccgccacgcc caggaccagg
     6661 gccgagaaga tcaacggcac caccagcatg aagagcaggc ggaggaagat ctggccgatc
     6721 ggacccgtga cgtagcggat gaagccgtcg agcgacgggt tgtccgcccc gaacaggtag
     6781 tgggccgcga ccccggcgcc ggccccgagg aggccgccga tcaggatctt atggtggaga
     6841 ggaaggcgca tgctcctcct gacgctgtcg ggctgtccga agcgggggaa tgctagagga
     6901 aaaaccgtcc ggcgccaact ccccccggct ccgggccctc ccggagcctc cccgccgccc
     6961 tgcggcgttg cgcgctgcgg gccgccgtgg cagacttccg gggctttcgg atcgtccaca
     7021 cacgagcccg ccgcgggcgg ctctttccag tctgggggtt tcctctgctg cgaggcatct
     7081 tcaagaccaa gtcgctcaac gccatcctgg cgtcggcgga ggcgccgcac acggccctga
     7141 accggtcgct gggaacgctc cagctcactc tgctcggcat cggcgccatc atcggcgcgg
     7201 gcatcttctc gaccgtgggc accgccgccg ccggcggcgc cagccacctg ggggccggcc
     7261 cgggcctggt gctgtccttc gtcatcaccg ccatcacctg cggcttcgcg gcgctctgct
     7321 acgccgagtt cgcctcgatg gtgccggtct cgggctcggc ctatacctat tcctatgcca
     7381 ccctcggcga gctggtcggc tggatcatcg gctgggacct gatcatcgag tacgccgtcg
     7441 gcaacatcgc cgtggccatc tcctggtcgg gctacttcca ggagctgctg cgcggcttcg
     7501 gcctcaactg gcccggctgg ctcggcaccg actaccggac cgccctccag gccgccggcc
     7561 aggtggcggc ggcgcgcgcc gaggggaccg accccgccgc cctgggcctg gcggtggagc
     7621 gcggcgccca ggccctggcc gaggcccccc gcctgttcgg catgcccctg gtcttcaacg
     7681 tgccggcctt cctcatcgtc atgctgatca ccgtcgtgct ggtggtgggc atccgcgaga
     7741 gcgcctggtt caactcctcg atggtggtgt tgaagctcgc catcatcgcc ttcttcatcg
     7801 gcctgggcgc cttctacgtc cagccggaga actggacgcc gttcgccccc aacggcttcg
     7861 tcggcatcgc cggggcggca gccatcatct tctttgccta catcggcttc gacgccgtat
     7921 cgacggcggc ggaggagacc aaggacccgg ggcggtccat gccgatcgcc atcatcgcca
     7981 gcctggcggt ctgcacgctg atctacatcg cggtggcgct ggtcctcacc ggcatgctgc
     8041 cgtggacgca gctcggcacc gccgagccgc tcgccaccgc cttctcggcg ctgggcatgg
     8101 gctggccggc gctgatcatc tcgctcggcg ccgtcttcgc caccacctcg gtgctggtcg
     8161 tcttccagct cggccagccg cgcatcttct tctccatggc gcgcgacggc ctgctcccct
     8221 cctgggccgc ccgggtgcat ccgcgctacc ggacgccgca catcaccacc tggatcaccg
     8281 gcatcgtcgt ggcgttcttc gccggcgtcg ccaacatcaa cgaggtggtg gagctgacca
     8341 acatcggcac gctcttcgcc ttcctcctgg tctgcgtcgg catcaccgtg ctgcgctaca
     8401 aggacccgga ccggccgcgc cgcttccgcg tgccgctcgg cccctggatc atcccgatgc
     8461 tgggcgccgc ctcctgcatc ttcctgatgg tctacctgcc gccggcctcg tggtggcggt
     8521 tcatcggctg gctggtgctc ggcatggcgg tctacttcgc ctacggctac agccacagca
     8581 cggtcgggcg cgaggccggc cgctcgccgc gcccgcggct gcccgagaag ctggcggcgg
     8641 cggggttcct ctccgccggc atcggcctgt tcaccatccc gcacgacgcc ggcctgcccc
     8701 gtctgctcca ggaggcggcg acggccggcg atcccgacca cgaccgcgcc ctcgtcggcc
     8761 tgatgctcat cgccgccggc ctggtgctcg tcgtcatcgg agcgct
//
LOCUS       DASPJI010000854         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_456072, whole genome shotgun sequence.
ACCESSION   DASPJI010000854 DASPJI010000000
VERSION     DASPJI010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456072"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="VEL74_24045"
     CDS             <1..1456
                     /locus_tag="VEL74_24045"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HYG65677.1"
                     /translation="GAVSVTREDRLLHSRGRGFADLVRLRTGTLPALPDAVARPAGAN
                     EVEEVLRTCSRDGIRVIPWGGGTSVTGGVNVVPGPGPAVVLDLERLSGLIRLDERSGL
                     ASFRAGTTGPAVESALAQSGFTLGHFPQSWELSTLGGWVATRSSGQESLGYGRIEDMT
                     AGLELVAPGGRLSLRAVPASAAGPDLRQLVLGSEGRLGVITEVTMRVRPRPSSRAVMA
                     LLVPSWEEGMDAIRELVLAGLPLNLLRLSDADETAVAMAVGLGGHAWGPLAKGWLRLR
                     GAGPGASLLLCGAAGKERLVRRTLDEAAALLKRQHAVSLGDGPGRRWLADRFRHPYLR
                     DGLLDLGFATDTLETAAPWSALPRLYQVVRGALAGALGEGEDEVPVLCHVSHVYRDGA
                     SLYFTFFFRLPEDPQEAIERWATLKRVATGALTGAGGTVSHHHGVGAWHAPWYPREAG
                     EAGVRVLAAAASELDPQGVLNPHVLLDPADRLEV"
     gene            1481..1954
                     /locus_tag="VEL74_24050"
     CDS             1481..1954
                     /locus_tag="VEL74_24050"
                     /inference="COORDINATES: protein motif:HMM:NF021487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HYG65678.1"
                     /translation="MELDEVMRVLGSFESEGVDYIIVGGVALNFHGLIRATEDLDLFI
                     RPEPENIERLRRALRSIYDDPSIEEISADDLLGDYPAVRYYPPSGEVFLDILTRLGEF
                     ASYDDLEAQSIEVEGVHVRVASPRSLYWLKKDTMREIDRADAEALREKFHLDETS"
     gene            2258..>2502
                     /locus_tag="VEL74_24055"
     CDS             2258..>2502
                     /locus_tag="VEL74_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYG65679.1"
                     /translation="MFHPQWRRQALAGLGEPFDLVILGGGITGCGILLDAAQRGLRVL
                     LIEKDDVASGTSSRSSKLIHGGLRYLKQLQFGVTRQA"
BASE COUNT          353 a          870 c          882 g          397 t
ORIGIN      
        1 gggcgccgtc tcggtcaccc gcgaggaccg gctgctgcac tcccgcggcc gcggcttcgc
       61 ggacctggtc cggctgcgca ccgggacgct gccggcgctg ccggacgcgg tggcacggcc
      121 ggccggcgcc aacgaggtgg aggaggtgct ccgcacctgc agccgcgacg gcatccgggt
      181 gatcccctgg gggggaggga cgtcggtgac cggcggcgtc aacgtggtgc ccgggccggg
      241 tccggccgtg gtgctggacc tcgagcggct ctccgggttg atccggctcg acgagcgctc
      301 cggcctcgcc agcttccgcg ccggcaccac cgggccggcc gtggagtcgg cgctggcgca
      361 gtcgggcttc acgctcggcc acttcccgca gtcctgggag ctctcgaccc tcggcggctg
      421 ggtcgccacc cgctcctcgg gccaggagtc gctgggctac ggccgcatcg aggacatgac
      481 cgcggggctc gagctggtgg cccccggcgg ccggctgtcg ttgcgggcgg tgcccgcctc
      541 cgccgccggg ccggacctgc gccagctcgt cctggggagc gaggggcgcc tgggggtgat
      601 caccgaggtg acgatgcgcg tgcgtccccg gccgtcgtcg cgcgccgtga tggccctgct
      661 cgtcccctcc tgggaggagg ggatggatgc catacgggag ctcgtgctcg ccggcctgcc
      721 cctgaacctg ctgcgcctgt cggacgcgga cgagaccgcg gtcgccatgg ccgtcggatt
      781 gggcggccac gcctggggcc ccctcgccaa gggctggctt cgcctgcgcg gcgccgggcc
      841 gggcgccagc ctgctgctct gcggcgccgc gggcaaggag aggttggtcc gccgaacgct
      901 ggacgaggcg gccgccctgc tcaagcgtca acacgcggtg tcgctgggcg acggcccggg
      961 ccgccgctgg ctggcggacc gcttccgcca cccctacctg cgcgacggcc ttctggatct
     1021 cggcttcgcc accgacacct tggagaccgc ggccccctgg tcggcgctgc cgcggctcta
     1081 ccaggtggtg cgcggggccc ttgcgggcgc gctcggtgag ggagaggacg aagtgccggt
     1141 gctctgtcac gtctcccacg tctaccggga cggcgcctcc ctctacttca ccttcttctt
     1201 ccgtctgccg gaggacccgc aggaggccat cgagcgctgg gcgacgctca agcgcgtggc
     1261 caccggcgcc ctgaccggcg ccggcggcac cgtctcgcac caccacggcg tgggcgcctg
     1321 gcacgccccc tggtatccgc gggaggccgg agaggccggc gtccgcgtcc tcgccgccgc
     1381 cgccagcgag ctcgatcccc agggcgtcct caatccccac gtgctcctgg acccggcgga
     1441 ccggctggag gtctgaacct cgtaccaagt ggagcagacc atggaactgg acgaggtgat
     1501 gcgagtgctg gggagcttcg aaagcgaagg ggtcgactac atcatcgtcg ggggcgttgc
     1561 tctgaatttc cacggtctca tcagagcgac ggaagatctc gatctgttca tccgtccgga
     1621 gccggaaaac atcgaacggc ttcgcagggc actgcgatcg atctacgatg accccagcat
     1681 cgaggagatc tcggccgacg atctcctggg agactatccc gcggtccgct actatcctcc
     1741 cagcggcgag gttttcctcg acattctcac gcggcttgga gagttcgcct cctacgatga
     1801 cctggaggcg cagagcatcg aggtagaggg tgttcacgtg agagtggcga gcccgcgaag
     1861 cttgtactgg ctcaagaagg acacgatgcg ggagatcgac cgcgctgacg ccgaggcttt
     1921 acgtgagaag tttcatctcg acgagacctc ctgaaaacga ttccaacccg aggctctgac
     1981 catgcccatt cagaagttcc ggaatctgga cgcgatgcga aaagctctct ggcagagctc
     2041 gtccggtgaa gacctcccgc gccgtatccg gagactctgg gcccgctcag tggccatcag
     2101 cccgagagtc tacccgcgcg gtgtcttcaa gtaccggagc ctcgaggaag cacagcgggc
     2161 acgggagcag atggtcactg acaatgtgca gcgtcttctc caggagcggc tcgccgcgga
     2221 gaagatccgc aacgattcct gaggcctttg gcgtactgtg ttccaccccc aatggcgccg
     2281 gcaggcgctc gccgggctcg gggagccttt cgacctggtg atcctgggcg gagggatcac
     2341 cgggtgcggc atcctcctcg acgcggcgca acgggggttg cgggtgctgc tgatcgaaaa
     2401 ggatgacgtc gcctcgggca cctcgtcgcg gtcctccaag ctcatccacg gcggcctgcg
     2461 ctatctcaag cagctccagt tcggcgtcac ccgccaggcc at
//
LOCUS       DASPJI010000855         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_54472, whole genome shotgun sequence.
ACCESSION   DASPJI010000855 DASPJI010000000
VERSION     DASPJI010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54472"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VEL74_24060"
     CDS             <1..1187
                     /locus_tag="VEL74_24060"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HYG65680.1"
                     /translation="RLRGLLVTGEVALALLLLVGAGLMIRSFLHLQSTDLGFRPERLL
                     TMRINLYGEKRPGPPQWGAFFQELTTRAQALPGVERAAVVLLRPLSGPIGWDYDFMAE
                     GQSPDERQTNPTANHERVSPGYFATMGIPMRRGRDFTWADAAGSPLVAIINESTARRL
                     WPGQDPLGKRLRWGRGEAETPWLTVVGVVGDVRYRDIQALRPDIYVPFLQDPHWSMDL
                     MVRADSDPLAIAQPMQEVIRAIEPDQPVANVTTMEEAIGRSVARPRLRTVILATFAAL
                     ALLLSAVGLYGIIAYSVAQRRREIGIRMALGADRRAVLAMVLRQALALTLAGLAAGLV
                     LAFGVLATGWISELLFGVRSTDFLTLSIVPLVLIAVTLAASLLPALRATRVDPLVALR
                     YE"
     gene            1187..1720
                     /locus_tag="VEL74_24065"
     CDS             1187..1720
                     /locus_tag="VEL74_24065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HYG65681.1"
                     /translation="MTVHSPNSDADYERLRDDLKRSVARVCPRWLADRSEDLVQVALM
                     RVMEIRRKSEGSAEFSSYYLKKAAYSALIDEIRRLRRRQEVSMEGDEETEFDPAAETP
                     DPERASAGKEVGRAIRDCLGLMVRPRKLAVTLNLQGHSVPEIGRLLGWTGKKAENLVY
                     RGMADLRGCLEGKGLGR"
     gene            1893..>2337
                     /locus_tag="VEL74_24070"
     CDS             1893..>2337
                     /locus_tag="VEL74_24070"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HYG65682.1"
                     /translation="MNPNEQELARWRAAFACPAEDVPAPEACPAPERIWAAVRGELPA
                     DEIREIVEHTAVCAACAEDWRLAMDLGRESGAAEEARPLAPVVYGRFQRWRPVAAAVA
                     MAAALAIVVGIRSDIDRPVEEPVYREAQETVVRSLVPAGRPLPRRE"
BASE COUNT          316 a          839 c          829 g          353 t
ORIGIN      
        1 cccgcctccg cggcctgctg gtgacgggcg aggtcgcgct ggccctgttg ctgctggtcg
       61 gcgccggcct gatgatccgc agcttcctgc acctccagtc caccgacctc ggcttccggc
      121 ccgagcggct cctgacgatg cgcatcaatc tctacggcga gaagcgcccg ggaccgcccc
      181 agtggggcgc cttcttccag gagctgacca ccagggccca ggccttgccg ggtgtggagc
      241 gggccgcggt ggtgctgctg cggccgctct cgggccccat cggctgggac tacgacttca
      301 tggccgaggg gcagtcgccg gacgagcggc agaccaaccc caccgccaac cacgagcggg
      361 tgagcccggg ctacttcgcc accatgggca tccccatgcg ccgcggccgg gacttcacct
      421 gggccgacgc cgcggggtcc cctctcgtcg cgatcatcaa cgagtccacg gcgcgccgcc
      481 tctggccggg ccaggatcct ctcggcaaac ggttacgctg ggggcgcggc gaggccgaga
      541 cgccctggct caccgtggtc ggcgtggtcg gcgacgtgcg ctatcgcgac atccaggcgt
      601 tgcggcccga tatctacgtg cccttccttc aggaccccca ctggtcgatg gacctgatgg
      661 tgcgtgccga ctccgacccg ctggccatcg cccagccgat gcaggaagtg atccgcgcca
      721 tcgagccgga ccagccggtg gccaacgtca ccaccatgga ggaggcgatc ggccggtcgg
      781 tggcccggcc gcgcctgcgt accgtgatcc tggcgacctt cgccgccctg gcgctgctgc
      841 tttcggcggt aggcctctac ggcatcatcg cctattcggt ggcccagcgg cgccgcgaga
      901 tcggcatccg catggccctc ggggccgacc ggcgggccgt cctcgccatg gttctgcgcc
      961 aagccctcgc cctcaccctg gccggcctcg ccgccggcct ggtcctcgcc ttcggggtcc
     1021 tggccaccgg ctggatctcg gagctgctct tcggcgtgcg ttcgaccgac ttcctgaccc
     1081 tttccatcgt gccgctggtg ctgatcgccg tgacgctggc cgcgagcctg ctgcccgctc
     1141 tccgggcgac ccgcgtcgat ccgctggtgg cgctacgata cgagtgatga ccgtccactc
     1201 cccgaattcc gatgccgatt acgagcgcct gcgggacgac ctgaagcgct ccgtggcccg
     1261 ggtctgcccg cgctggctgg ccgaccgctc cgaagacctg gtgcaggtag ccctcatgcg
     1321 ggtgatggag atccgccgca agagcgaggg gagcgccgag ttctcctcct actatctgaa
     1381 gaaggcggcc tacagcgccc tgatcgacga gatccggcgc ctgcgccgcc gccaggaggt
     1441 ctccatggag ggggacgagg agacggagtt cgatccggcc gccgagaccc cggacccgga
     1501 gcgcgcctcc gccggcaagg aagtcggccg cgccatccgc gattgcctgg gcctcatggt
     1561 ccgcccccgc aagctggcgg tgacgctcaa cctccagggg cacagcgtgc cggagatcgg
     1621 ccgcctcctc ggctggaccg gcaagaaggc ggagaacctg gtctaccggg gcatggcgga
     1681 cctccgcggc tgcctcgagg gcaaggggct cggccggtag cttcaccggt attttcatgt
     1741 aagtgaggga aacgcagggc tcacggcgtt ctaaccagtg agcccgcccg agggcgccct
     1801 cagagtgggg gcggtacctc cagaggcggc aggaggcttt tcttgatcta cgttcagttc
     1861 gagcacttcc ggagcaggat ggagagaggg cgatgaaccc caacgagcaa gagctggcgc
     1921 gctggcgcgc ggcgttcgcc tgtcccgcgg aggacgtccc ggccccggag gcctgtccgg
     1981 cgcccgagag gatctgggcg gcggtgcgcg gcgagctccc ggcggacgag atccgcgaga
     2041 tcgtggagca cacggcggtc tgcgccgcct gcgccgagga ttggcgcctg gccatggacc
     2101 tcggccggga gagcggcgcg gcggaagaag ccaggccgct ggcgccggtc gtctacgggc
     2161 ggttccagcg ctggcgcccg gtcgcggcgg cggtggccat ggcggcggcg ctggcgatcg
     2221 tcgtcggcat ccggagcgac atcgaccggc cggtggaaga gcccgtctat cgcgaagccc
     2281 aggagaccgt cgtccggtcg ctggtgcccg ccggccggcc tctcccacgc cgcgagg
//
LOCUS       DASPJI010000856         4219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_227177, whole genome shotgun sequence.
ACCESSION   DASPJI010000856 DASPJI010000000
VERSION     DASPJI010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227177"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VEL74_24075"
     CDS             complement(<1..789)
                     /locus_tag="VEL74_24075"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HYG65683.1"
                     /translation="MTPSPALPSGLRRDTLLNLAGQLLPLAVAVAAVPATTAGLGVER
                     FGVLALAWTVLGSFNVFDLALGRAVTRFAAEALAQDEPERLPGLLWSATGLQAALGIV
                     AAAAFAAASPLLAGAVLQAPPPLADEIRRTFLILALSFPVTLVTGCFRSLLEAAGRFD
                     LVNLVRAPAGAATFAIPWLGAAAGWDLPAIIGLLVLTRALVLVVHWRLCRRALPVLVA
                     ARAWPAREPLARLARFGGWITASNVIPPLLTLVERFLLGALVSLA"
     gene            complement(786..1733)
                     /locus_tag="VEL74_24080"
     CDS             complement(786..1733)
                     /locus_tag="VEL74_24080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG65684.1"
                     /translation="MKTPDPESRPALSVVIPAYNSHETIGGCLEALSRQTFRSFEVIV
                     VDSGPDGATQRMMERFPWVRYERSERRLLPHAARNRGVELARGTVLVFTDPDIYAHPD
                     WLERLAAAHLGTGEVIVGALACYGDRWLDHGIHLCKFSKWLPGGPPREVDMSPTANML
                     LPRSEFEAAGGLPGDEFLGDVTLSRTLNGRGRRLRFAPDAVVEHHHVQSFRDFMTERY
                     TRGKMHGDLRLGWLVGRRAAALLYLSVTVLPVRLPRILALVGMHAGRAGQTGIYLKTL
                     PLVVAGHAASLAGEAVAYLRRLAPKLQRAQRQPDPARSR"
     gene            complement(1747..2697)
                     /locus_tag="VEL74_24085"
     CDS             complement(1747..2697)
                     /locus_tag="VEL74_24085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65685.1"
                     /translation="MSKDLTVVLAVGARRDRAVGCLASILDQEPIDRLEVLLFELGPE
                     GVPPVPGSDHPAVRVLRRPPDTLYSAAKTEAVRLASAPVVAFIEEHVRTHPGWAAALI
                     EAHRGPWAGVGCEVHNGNPGVALSRVIQLINYHPWMPPAPRAEFGMLPGHNSSFKRDV
                     LLSYGDGLQDLLRAEVLLHVRLHRDGHRLLLEPAARISHINESTLGSAMRGRFLWNRI
                     YAPMRARAFGWSAARRLFYVAGVPLVPLYSLARLFLFLARRRPALLPRAVAGSPAIFM
                     AQLAAGAGQSLGLLLGTGDAEAEFSLFELNEYRAYEPAEK"
     gene            3569..>4219
                     /locus_tag="VEL74_24090"
     CDS             3569..>4219
                     /locus_tag="VEL74_24090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65686.1"
                     /translation="MTDQPILDPLYREAVAAIDAGNVPALERMLAEQPRLLHERLAAP
                     GAWLIDKVGEAALVDFFSQPYLLWFVAEDPVRNGRLPGNIAEVARAIIQAARREGVTS
                     LQEQLDYCLRLVSWSWIARESGVQNGLIDVLVDAGASLSETITHDALINGNFGAAARL
                     VERGAELTLATALCLERWEDAARLAETASGRDKQVALVLASLHGKAEALTRLIAPGV"
BASE COUNT          650 a         1417 c         1610 g          542 t
ORIGIN      
        1 ggccagcgag accagcgccc ccagaaggaa ccgctccacc agcgtgagca gcggcgggat
       61 gacgttcgag gccgtgatcc agccgccgaa gcgggccagc cgggccaggg gctcgcgggc
      121 gggccacgct ctcgccgcta ccagaaccgg cagggcgcgc cggcagagcc gccagtggac
      181 gaccagcacc agcgcccggg tgaggactag caggccgatg atggcgggga ggtcccaccc
      241 ggccgccgct cccagccagg ggatcgcgaa ggtggccgcc ccggccggcg cccgcaccag
      301 gttcaccagg tcgaagcgcc ccgccgcctc gaggaggctg cggaagcagc cggtgacgag
      361 ggtcaccgga aaggagaggg cgaggatcag gaaggtgcgc cggatctcgt ccgccagcgg
      421 cggcggcgcc tggagcacgg cgccggcgag gaggggcgag gcggcggcga aggccgccgc
      481 ggccacgatc ccgagggcgg cctggaggcc ggtggcgctc cacagcaggc cgggcagccg
      541 ctccggctcg tcctgggcca gcgcctcggc ggcgaaccgg gtgaccgcgc ggccgagggc
      601 gaggtcgaag acgttgaacg agcccaggac cgtccaggcg agggcgagca cgccgaagcg
      661 ctcgacgccg agcccggcgg tggtggcggg cacggcggcc accgccaccg ccagggggag
      721 gagctgcccg gcgaggttga gcaacgtgtc ccggcggagg ccggagggga gggcggggga
      781 gggcgtcacc ggctccgggc cgggtccggc tgccgctgag cgcgctggag cttcggcgcg
      841 agccgccgga ggtaggcgac ggcctcgccg gcgagagagg cggcgtgccc ggcaacgacc
      901 agggggagag tcttcagata gataccggtc tgtccggcgc gcccggcgtg catccccacc
      961 agcgccagga tgcgggggag gcgcacgggc aggacggtga ccgacaggta gaggagcgcc
     1021 gcggcccgcc gccccaccag ccagcccagg cgcaggtcgc cgtgcatctt gccccgggtg
     1081 tagcgctcgg tcatgaagtc ccggaagctc tggacgtggt ggtgctccac cacggcgtcc
     1141 ggcgcgaagc ggagccgccg gccgcgcccg ttcaaggtgc gcgacagggt gacgtcgccc
     1201 aggaactcgt cgcccggcag gccgccggcg gcttcgaact cgctccgcgg cagcagcatg
     1261 ttggcggtgg ggctcatgtc cacctcccgg ggcgggccgc cggggagcca cttgctgaac
     1321 ttgcagaggt ggatcccgtg gtccagccag cggtcgccgt agcaggcgag ggcaccgacg
     1381 atcacctcgc cggtgccgag gtgggcggcg gcgagccgct ccagccagtc gggatgggca
     1441 tagatgtcgg gatcggtgaa gaccaggacc gtgccgcggg ccagctcgac gccccggttg
     1501 cgggcggcgt gggggaggag ccgccgctcg ctacgctcgt agcgcaccca ggggaagcgc
     1561 tccatcatcc gctgggtggc gccgtccggg ccgctgtcca ccacgatcac ctcgaaagac
     1621 cggaaggtct ggcggctcag ggcttccagg cagccgccga tggtctcgtg cgagttgtag
     1681 gccgggatga cgaccgacag ggcaggacgg ctctccgggt ccggggtctt catcgctctc
     1741 accctctcac ttttccgcgg gttcgtaggc ccggtactcg ttcagctcga aaaggctgaa
     1801 ttcggcctcc gcgtcccccg tcccgaggag gagccccagg ctctggccgg cgccggcggc
     1861 gagctgggcc atgaagatcg cgggcgagcc ggcgacggcg cgcggcagca gcgcgggtcg
     1921 ccgccgggcc aggaagagga acagccgcgc cagcgagtag agcggcacca ggggcacgcc
     1981 ggccacgtag aacagccgcc gggctgcgga ccagccgaag gcgcgggcgc gcatcggcgc
     2041 gtagatgcgg ttccagagga agcgtccgcg catggcgctg ccgagcgtgc tctcgttgat
     2101 gtgcgagatc ctggccgccg gctcgaggag cagccggtgg ccgtcccggt ggaggcgcac
     2161 gtggaggagc acctcggccc gcaggaggtc ctggagcccg tcgccgtagg agaggaggac
     2221 gtcccgcttg aacgacgagt tgtggcccgg gagcatgccg aactcggcgc gcggcgccgg
     2281 tggcatccac gggtgatagt tgatgagctg gatgacgcgg ctgagggcga ccccggggtt
     2341 gccgttgtgg acctcgcaac ccacgcccgc ccacggcccg cggtgcgcct cgatgagggc
     2401 cgcggcccag ccgggatggg tacgcacgtg ctcctcgatg aaggcgacga ccggcgccga
     2461 cgccaggcgc accgcctcgg tcttggcggc ggagtagagg gtgtcgggcg gccggcggag
     2521 cacgcgcacc gcggggtggt cgctccccgg cacgggtggc acgccctccg gcccgagctc
     2581 gaagagcagc acctccaggc ggtcgatcgg ctcctggtcg aggatggatg ccaggcagcc
     2641 gacggcgcgg tcccgccgtg cgcccaccgc caggacgact gtgaggtcct tggacatata
     2701 tggatgtcga gaagctggcc ggagactcta gcagaaaagg gggcgggttg gagtatcctg
     2761 ctgacggcgt ctggaagctc ggcgcctccg ggctgacgga atggacgtcg cctcccctta
     2821 tggggcggct cgctccctaa aaggggggac caccgtccag acccacccca atgactaaag
     2881 atcgaatttg ataggcactc caaggatggc ttggcgaagt tggggcaccc cggtctccgc
     2941 gccgcgctcc gtctggccag cggttggccg agcgaggcct atggttggcc gagtgcggcc
     3001 ggtgacaggc cgcgcggtgc ctatgacaga cctggtgcgg ccgatgtcag gccgcgcggg
     3061 gcccatggca ggctgggtga ggcccaccgc aggccgcatg ctgccgatga caggccgaag
     3121 gatgcccatg gctgaccttg tggggccgat gactggcctc gtgctgcccg gccacggcgg
     3181 agcgaagccg gccggcgatc ggccgggtca gcgaggcgca gagggcgagg gagcggcagg
     3241 acgcgaggaa cggcgccgga tcgccccgct ccggcttctc gccctgatgt cgcggtatcc
     3301 tcgagcttcc aacgaacctg cgcaagggca ggagcaagga gacccatgag acgccggatg
     3361 ctgtttctcg ccgttaccct cgcgcttttg agcacgattt tgacccaccc caccgttccc
     3421 gtgagctcct gcgacgagtg cgcggagagc tgcaactcga tcccgatggc gacggaggat
     3481 tgcctgcagc tctattgccc tgaatgctcc ggctcctcct agacaagtgg ggggcacgga
     3541 gtacctggac accgagtaaa ggcgcgtcat gacggatcag ccgattctcg acccgttgta
     3601 ccgcgaagcc gtcgctgcga tcgacgccgg gaacgttccg gctctggaac ggatgctggc
     3661 cgagcagccc aggctgctcc acgagcggct ggccgctccc ggcgcctggc tgatcgacaa
     3721 ggtgggcgag gccgccctcg tcgatttctt ctcgcagcct tatctgctct ggttcgtggc
     3781 cgaagacccg gtccgcaacg gccggttgcc gggcaacatc gcggaggtgg cccgcgcgat
     3841 cattcaggcc gcgcgacgcg agggggtgac gagcctccag gaacagctcg attattgcct
     3901 gcgcctggtg tcctggtcgt ggatcgccag ggaatccggg gtgcaaaacg gcctcatcga
     3961 cgtgctggtc gacgccggcg cctctctctc cgaaaccatc acccacgacg cgctcatcaa
     4021 tggcaacttc ggcgccgccg cgcgcctcgt cgagcggggg gccgagctga cgctggcgac
     4081 cgcgctctgt ctggaacgtt gggaggacgc cgcgcgcctc gcggagacgg ccagcgggcg
     4141 cgacaagcag gtcgcgctgg tcctcgcctc gctccacggc aaggccgagg cgctcacccg
     4201 tctgatcgcc ccgggggtg
//
LOCUS       DASPJI010000857         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_165190, whole genome shotgun sequence.
ACCESSION   DASPJI010000857 DASPJI010000000
VERSION     DASPJI010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165190"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            867..1238
                     /locus_tag="VEL74_24095"
     CDS             867..1238
                     /locus_tag="VEL74_24095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65687.1"
                     /translation="MASINSLADVLLDWEGLLAALLEHPELAELLGAERVVLERDLAE
                     VRALKARQEAQRAGAQEMTQRIKEVVTHGKEVAITIRAVAKGKLGYRNERLVHFKVAP
                     VRKRPRKPAAANPPAGEPAAE"
     gene            1313..1657
                     /locus_tag="VEL74_24100"
     CDS             1313..1657
                     /locus_tag="VEL74_24100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65688.1"
                     /translation="MARTGFESNLNDWENLLNQVRELAHKYPYLESFRIELEEAAGKA
                     RAAKAQQMALQAASQQATHQVATALVQGRDAAIRMRSYLRGCLGFRNEELVRFGVAPI
                     RKRGPRKVRTQV"
     gene            complement(1818..2291)
                     /locus_tag="VEL74_24105"
     CDS             complement(1818..2291)
                     /locus_tag="VEL74_24105"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG65689.1"
                     /translation="MTYRQAALSDIPAMARLWMDGGGGEGGTSQDRMERYLKGEHHPQ
                     HALKPRVIYIALEGDSLVGYIAGHLTRRYGCDGEIQWIYVALEHRAQGVAPELLRLLA
                     EWFAQQDAPRVCVNVDPANTTARRFYMRHGAESLNEHWLVWSGIKVVLASESLPS"
     gene            complement(2288..>2773)
                     /locus_tag="VEL74_24110"
     CDS             complement(2288..>2773)
                     /locus_tag="VEL74_24110"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HYG65690.1"
                     /translation="TSLRGLAALRREEFDAYRRETDRFPADRFETWGMPYHRNRFQGR
                     RARQAQASEDGLLRGIGCCPGVVTGPVKVLRAPSEDARLAGEILVAERTDPGWVPLYP
                     SVSGLLIERGSILSHSAIVAREMGIPTIVGIPGLVSTLKTGQTVTMDGAAGTVRVEEP
                     S"
BASE COUNT          473 a          934 c          924 g          442 t
ORIGIN      
        1 tggaggcggc cgggtcggtg accctcgagc ggtgcggggc tatcctcgac gagcgggcgg
       61 ccactccgga gtaggacatc tttatctata taggggcgcc accttgccgg tggcgtcccg
      121 cttctcctcc gcccccctcc cgaagaagaa aacccccctc ctgacctccc ccgctcccta
      181 ccgcccacct cccagggggg aggcacgacc gcccgctggg catctccccg gaggcttctc
      241 ccctcgaggg ccctcccgcg aggcacccgt accctgtacc ccggaggcgg aggggcttcc
      301 gagcccccct ccgcctccac ttgggcgagg ctccgctaaa agaggcgcgc cattggaccc
      361 ggcggcgccc cttcagattc agttttcaaa gaaccccagc aaccaatggc ccggcggccc
      421 cgaggagcct tcggcctgcc ctggagcggt acccaccagc ccctgcgggc cagcagccgg
      481 cccttgctcc agtcctgctt gccctggagg gcaggtcggc cgcccctgag ggcattcagc
      541 atgcgccaca gggcctgtcg gctgcccctg agggcgtggg tgggcctcca ggggcctcac
      601 ctgagcccgt gggggccggc cctgggcgct ccagggcctc gcacccaccc cgcggggccc
      661 gggaagcata gcgagtggag ccggtacgag tctacagact gaaactggca tggacaagat
      721 ggattctaca agattctttg ccttacccct tgacagccgg ttcagacctg tctagagtcc
      781 tcgatgtcga tcgattccgg tccttcagag gccgggacga ttatccggcg ccaatccagg
      841 cccggaatct tcaaaggaga agcagtatgg caagcatcaa ctcattggcg gacgtcttgt
      901 tggactggga gggcctgctg gccgccctcc tggagcatcc ggagctcgcg gagctcctcg
      961 gagccgaacg cgtggtcctg gagagggacc tggccgaggt gcgggcgctc aaggcgcggc
     1021 aggaggcgca gagggccggt gctcaggaga tgacgcagcg gatcaaggag gtggtgaccc
     1081 acggcaagga ggtggcgatc accatccgcg ccgtggcgaa gggcaagctc ggctaccgca
     1141 acgagcggct ggtccacttc aaggtggccc cggtccgcaa gcgtccgcgc aagccggcgg
     1201 cggccaatcc ccccgctggc gagcccgccg cggagtagca ccctgaggac gtaggacctg
     1261 atccgggggg cgtgctccgg cacgcccccc actttcaaga gaggacacaa tcatggcgag
     1321 aacgggtttc gaatccaacc tcaacgactg ggagaacctg ctcaaccagg tccgggagct
     1381 ggcgcacaag tatccctatc tcgaatcctt ccggatcgag ttggaggagg ccgccgggaa
     1441 ggcacgtgcg gcgaaggcgc agcagatggc gctccaggcc gccagccagc aggcgacgca
     1501 tcaggtggcc accgccctgg tacagggacg ggacgcggcg atccgcatgc gcagctatct
     1561 gagaggctgt ctggggttcc gcaatgaaga gttggtccgc ttcggcgtgg ccccgatccg
     1621 caagcgtgga ccgcgcaaag tccgtacaca ggtgtagaga acgaatcaag acggcatagc
     1681 agatcaacgg gcaggttcgt ctctgttgct ctttgacaac tgatggaaac gtccggagcg
     1741 cggccccctg ggaggaggag accagggccg ccgcgctccg gtacgctcgt ggtctctctt
     1801 caggtgtagg cttcggccta cgacggcaga ctctcacttg ccagcaccac cttgataccg
     1861 ctccacacca gccagtgctc gttgaggctc tcagctccgt gtcgcatgta gaaacgccgg
     1921 gcggttgtat tagccggatc gacgttcacg cagactcgtg gagcatcctg ttgcgcgaac
     1981 cactctgcca ggaggcggag caactccggg gcgacgcctt gcgcccggtg ctccagagcg
     2041 acgtagatcc actggatctc tccatcgcat ccgtaccgcc gcgtgaggtg tccggcgata
     2101 tagcccacga gagagtcgcc ctccagcgcg atgtagatca cgcggggctt cagcgcgtgc
     2161 tgtggatggt gctcgccctt caggtagcgt tccatccggt cttgggaggt ccctccctcc
     2221 ccgcctcccc catccatcca gaggcgggcc atggcaggga tgtccgatag ggcagcctgt
     2281 ctgtaggtca cgagggttcc tccacccgca ctgtccctgc cgcgccgtcc atcgtcacgg
     2341 tctggccggt cttcaacgtg ctgacgagtc cggggatgcc gacgatggtg ggaatcccca
     2401 tctcccgggc aacgatggcc gaatgcgaga ggatgcttcc ccgctcgatc aaaaggccgg
     2461 agaccgatgg atagagcggc acccagccgg ggtcggtgcg ctcggcgacc aggatctcgc
     2521 cggcgaggcg cgcgtcttcg gatggcgcgc gcaggacctt caccggtccg gtgaccacgc
     2581 cggggcagca gccgatgccg cgcaggaggc cgtcctccga agcctgggct tgccgcgcgc
     2641 gccggccctg gaaacgattg cggtggtagg gcatccccca ggtttcgaat cggtcggcgg
     2701 ggaaacggtc ggtctcgcgg cgataggcgt cgaactcctc gcggcggagc gcggcaaggc
     2761 cccggaggct ggt
//
LOCUS       DASPJI010000858         5019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_623978, whole genome shotgun sequence.
ACCESSION   DASPJI010000858 DASPJI010000000
VERSION     DASPJI010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623978"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2408)
                     /locus_tag="VEL74_24115"
     CDS             complement(<1..2408)
                     /locus_tag="VEL74_24115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HYG65691.1"
                     /translation="MPRSWLWKKTVDDEVSEETEFHLEMRTREYIARGLDPAAARDAA
                     LRRFGDLERVRATCREIGRRRDMDMRRREYFGELRQDVTFALRQLAANPGFTLIAVLT
                     LALGIGATTAIFSAVNAVVLRPLPVPEPERLMLITSQWRGERSSSSVPNYLALAERQR
                     SFSHLAAAQYAGFSLSEGESPELVLGARVTASFFDVYGVRPELGRVFGADEDQPGHEQ
                     VAVLSHRLWQRRFGADPALIGRDIRMNRQPYTVIGVMPERFDFTADSEELWVPIAFTP
                     ERKQVYDEHYLTVGGRLRDGVTLQQAEQEMAAVSESLRRLRPQENAERSLVVEPLMER
                     LIGDYGTRLLVLLGAVGLVLLIACGNVANLLLARGAVRAPELAIRASLGAGRGRIVRQ
                     LLTESAVLSLVSAAIGLVLAWWGVRTLVALSPEGVPRLEQTTLDGTVLAFTLAVSIAS
                     SAVFGLAPALRAARATGAALKEGGRGAAGAGRDWLRPTLIAAEVALAVLLLVGAGLLI
                     RSALELQRVQPGFRPEGVISARFTLPAADYQEPERVVATFGRLLEETARTPGVQAAAL
                     SSQVPMSPGGSSNGLLAEGKPVDTKEIVQSQFRVVTPGYFQAMGIPILRGRGFEASDR
                     RGGQKVMVVSESLAEALFPGQDALGRRVGCCEAGPDENTPDMKLIVGIAADTRWMGIT
                     EGVAPEFYLPVEQAPPAVWDWTQRSMYLVARTAGDPEALVPALRRVAGGIDRNVPLYE
                     VRTMEQRLAESLATARFNTLLLTLLGAIGLILSAVGIYGVIAYFVTQRTSEIGVRMAL
                     GATPR"
     gene            complement(2427..2759)
                     /locus_tag="VEL74_24120"
     CDS             complement(2427..2759)
                     /locus_tag="VEL74_24120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HYG65692.1"
                     /translation="MPQQASSLLHGTLDALILKTLVTGPRHGYAIARWIEEATDELLQ
                     IEEGSLYPALYRMERKEWIEAEWGVSEMNRRAKFYRLTPKGRQQLQVETEQWALFTAA
                     ISKVLLPA"
     gene            complement(2813..>5019)
                     /locus_tag="VEL74_24125"
     CDS             complement(2813..>5019)
                     /locus_tag="VEL74_24125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG65693.1"
                     /translation="GLIVLLAIPTIAALAQSQGRFPEHWLPWAALIAAVYLFWVARQQ
                     HHERWDSLAARYRLELRASDLERERQELEGSHAGLTRLVAERNRELERTSQDYRQIFE
                     CAHDAIIVFRPDDERVLAVNRRACEVYGFSREEFLRISLADISEDVERGKRQIGDTLA
                     RGVYYNFESVQFRKDGSRMFLEINASAIEYEGRRAILSINRDVTERRRAEELRLAKEA
                     AERAGRAKTQFLANMSHEIRTPMAGIVGLSDLLLQSGLDERQGDYARLIQSSAVSLLR
                     VIDDILDFSKIEAGKLTLEEVAFDLQATLREVVELLRLAAAARGTSLELATGDALPTW
                     VCGDPARLRQVLMNLVGNAVKFTEEGSVSVHAVTMGAGRVHIRVEDTGIGIPAESQGQ
                     LFEVFSQADDSTSRRFGGTGLGLAISRRIVEAMGGEIGFESAQGQGSTFWITVHLPPA
                     EPPVTAEVPAGRPDRPYRVLTAEDNPINQLVITEQLKAMGYEVTAVSNGLEAVSALES
                     AQGSSGSFDLVLMDCQMPHLDGYAATARIRQLPGAAGRVPVVALTAHVLREDLDKCLA
                     QGMDDYITKPFREEVLRRKLERWLGGRQPQAGRPQVGRVGPPAVPVDLLADEVLDEEQ
                     LESLRGLGRESGPDFLCRLVEQFRSRPFLDELRDAVERDDRAALHCRAHGLKGSSAFL
                     GARRLARLFAGLEGLSRDGTREECLRQIALIEAEHGRVLPRLAAALASPASP"
BASE COUNT          708 a         1742 c         1869 g          700 t
ORIGIN      
        1 cgcggcgtgg ctcccagcgc catccgcacg ccgatctcgg acgtgcgctg ggtgacgaag
       61 taggcgatca caccgtagat gcccaccgcc gacaggatca ggccgatggc tcccaggagc
      121 gtcaggagca gcgtgttgaa gcgcgcggtg gccagcgact ccgccagccg ctgctccatg
      181 gtccgcacct cgtagagcgg cacgttgcgg tcgatgcccc cggcgacccg gcgcagcgcc
      241 ggcaccagcg cctccgggtc gccggcggtg cgggccacga ggtacatgga gcgctgggtc
      301 cagtcccaga ccgccggcgg cgcctgctcc acaggcagat agaactcggg ggccaccccc
      361 tcggtgatgc ccatccagcg ggtgtccgcg gcgatgccca cgatgagctt catgtcggga
      421 gtgttctcgt cgggcccggc ctcgcagcag ccgacccgcc ggccgagggc gtcctggccg
      481 gggaagagcg cctcggcgag ggactcgctg accaccatca ccttctggcc gccgcggcgg
      541 tcgctggctt cgaagccgcg gccccgcaag atggggatgc ccatcgcctg gaagtagccc
      601 ggcgtgacga cgcggaactg cgactggacg atctccttcg tgtcgacggg cttcccttcg
      661 gccagcagcc cgttgctgct gccgccgggg gacatcggta cctgggagga gagggcggcg
      721 gcctggacgc cgggggtccg cgccgtctcc tccaggaggc ggccgaaggt ggcgacgacg
      781 cgctcgggct cctggtaatc ggcggcgggt agggtgaagc gggcgctgat cactccttcg
      841 ggccggaacc cgggctggac ccgctgcagc tccagggcgg agcggatgag caggccggca
      901 ccgaccagca ggaggacggc gagggcgacc tcggcggcga tcagggtcgg gcggagccag
      961 tcgcggccgg cgcctgccgc gccgcgtccc ccctccttga gcgccgcgcc agtggcgcgg
     1021 gcggcgcgta gcgccggggc gaggccgaag acggcgctgc tggcgatcga gacggcgagg
     1081 gtgaaggcga ggacggtgcc gtccagcgtc gtctgctcga gccgcggcac cccctcgggg
     1141 ctcagggcca ccagcgtccg caccccccac caggcgagca ccaggccgat cgcggcggag
     1201 accagggaca gcaccgcgct ctcggtgagg agctggcgga cgatccggcc gcggccggcg
     1261 ccgagcgagg cccggatcgc cagctcgggc gcgcgcaccg ctccccgcgc cagcagcagg
     1321 ttggcgacgt tgccgcaggc aatgagaagg acgagcccca ccgcgcccag cagcaccagc
     1381 agccgcgtgc cgtagtcgcc gatgagccgc tccatgagcg gctccaccac cagggaccgc
     1441 tccgcgttct cctgcgggcg gagccggcgc agactctccg acacggcggc catctcctgc
     1501 tccgcctgct gcaaggtgac gccgtcgcgc agccgccccc ccaccgtcag gtagtgctcg
     1561 tcatagacct gcttgcgctc gggggtgaag gcgatgggca cccagagctc ctcgctgtcc
     1621 gcggtgaagt cgaagcgctc cggcatcact ccgatcaccg tgtagggctg gcggttcatc
     1681 cggatatccc gtccgatgag ggccggatcg gcgccgaacc gccgctgcca caggcggtgg
     1741 ctgagcacgg ccacctgctc gtgtccgggc tggtcctcgt ccgctccgaa gacgcgcccg
     1801 agctccgggc gcacgccgta gacatcgaag aaggaggccg tgacccgggc gccgaggacc
     1861 aactcggggc tctccccttc cgagaggctg aagccggcgt actgggccgc cgccaggtgg
     1921 gagaaggagc gctgccgctc cgccagcgcc aggtagttgg gcaccgagga ggaggagcgc
     1981 tcgccgcgcc actggctggt gatcagcatc agccgctccg gctcggggac cggcagcggc
     2041 cgcagcacca cggcgttcac ggcggagaag atcgccgtgg tggcgccgat gccgagggcc
     2101 agcgtcagca cggcgataag ggtgaagccc gggttggcgg cgagctggcg cagggcgaac
     2161 gtcacgtcct gccgcagctc gccgaagtac tcgcggcgac gcatgtccat gtctctcctc
     2221 cggccgatct cacggcatgt ggcgcggacg cgctcgaggt caccgaagcg gcgcagcgcg
     2281 gcgtcgcgcg ccgcggccgg atcgaggccg cgggcgatgt actcgcgggt ccgcatctcg
     2341 aggtggaact ccgtttcttc ggacacctcg tcgtcgacgg tcttcttcca gagccaggag
     2401 cggggcatgg tcggtccggc gtgccctcag gcggggagca ggaccttgga gatggcggcg
     2461 gtgaagagcg cccactgctc ggtctccacc tggagctgct ggcggccctt cggagtcagc
     2521 cggtagaact tggcgcggcg gttcatctcc gacacccccc actcggcctc gatccactcc
     2581 ttgcgctcca tgcggtagag cgccgggtag agcgacccct cttcgatctg cagcagctcg
     2641 tcggtcgcct cctcgatcca gcgcgcgatg gcgtagccgt ggcgcggacc ggtgaccagc
     2701 gtcttgagaa tcagggcgtc cagggttccg tggagcaggc tgctggcttg ctgaggcatc
     2761 gtcctttccc ctagtgagtc taggtaaagg atagggagcg atacctagcc tgtcaagggg
     2821 aagcggggct ggcgagggcg gcggccaggc gggggaggac ccggccgtgc tcggcttcga
     2881 tgagggcgat ctggcgcagg cactcctccc gcgtgccgtc gcgggagagg ccttcgagcc
     2941 cggcgaagag ccgggcgagg cggcgggcgc cgaggaaggc gctgctcccc ttgaggccgt
     3001 gggcccggca gtggagggcg gcgcggtcgt cgcgctcgac ggcgtcgcgg agctcgtcga
     3061 ggaacgggcg gctgcggaac tgctcgacca gccggcagag gaagtccggc cccgactccc
     3121 ggccgaggcc gcgcaaggat tcgagctgct cctcgtccag cacctcgtcc gccagcaggt
     3181 ccaccggcac ggcgggcggc cccaccctcc ccacctgggg gcgtccggcc tgcggctggc
     3241 gcccgcccag ccagcgctcc agcttgcgcc gcaggacctc ctcgcggaag ggtttggtga
     3301 tgtaatcgtc catgccctgg gcaaggcact tgtcgaggtc ctcccgcagg acgtgggcgg
     3361 tcagcgcgac gacggggacg cgccccgcgg cccccgggag ctggcggatg cgggccgtcg
     3421 ccgcgtagcc gtcgaggtga ggcatctggc agtccatcag caccaggtcg aacgatccgc
     3481 tggatccctg ggccgattcc agggccgaga cggcttccag gccgttgctg accgcggtga
     3541 cctcgtagcc catggccttg agctgctcgg tgatgacgag ctggttgatc gggttgtcct
     3601 ccgcggtcag gacgcggtac gggcggtcgg gccggccggc cgggacctcc gccgtgaccg
     3661 ggggctcagc cggcggcagg tgcacggtga tccagaaggt ggagccttgt ccctgggcgc
     3721 tctcgaagcc gatctcgccc cccatcgcct cgacgatccg ccggctgatg gcgaggccga
     3781 gcccggtgcc gccgaagcgc cgggaggtcg agtcgtcggc ctgcgagaag acctcgaaga
     3841 gctggccctg ggactcggcg gggatgccga tgccggtgtc ctcgacccgg atgtggaccc
     3901 ggccggcgcc catggtcacg gcgtggacgc tcaccgagcc ctcttcggtg aacttgacgg
     3961 cgttgcccac caggttcatc agcacctgcc gcagccgggc gggatcgccg cacacccagg
     4021 tgggcagggc gtcgccggtg gcgagctcga gggaggtccc gcgggccgcg gcggccagcc
     4081 gcagcagctc caccacctcg cgcagcgtcg cctggaggtc gaaggccacc tcttcgagag
     4141 tcagcttgcc ggcctcgatc ttggagaagt cgaggatgtc gtcgatcacc cgcagcagcg
     4201 acaccgccga ggactggatg agccgggcgt agtccccctg gcgctcgtcg aggccgctct
     4261 ggagcagcag gtccgagagg ccgacgatcc cggccatggg ggtgcggatc tcgtgactca
     4321 tgttggccag gaactgggtc ttggcgcggc cggcgcgctc ggcggcctcc ttggcgaggc
     4381 gcagctcctc ggcgcggcgg cgctcggtga cgtcgcggtt gatgctgagg atcgcccgcc
     4441 gcccctcgta ctcgatggcc gaggcgttga tctcgaggaa catgcggctg ccgtccttgc
     4501 ggaactggac gctctcgaag ttgtagtaga ccccccgcgc cagcgtgtcg ccgatctgcc
     4561 gcttgccgcg ttcgacgtcc tcggagatgt cggcgaggga gatgcgcagg aactcctcgc
     4621 ggctgaagcc gtagacctcg caggcgcggc ggttgacggc gagcacgcgc tcgtcgtccg
     4681 gccggaagac gatgatcgcg tcgtgggcgc attcgaagat ctgccggtag tcctggctcg
     4741 tccgctccag ctcccggttg cgctcggcca ccaggcgggt gaggccggcg tgggagccct
     4801 cgagctcctg ccgctcccgt tccaggtcgg aggcgcgcag ctccaggcga taccgcgcgg
     4861 cgaggctgtc ccagcgctcg tggtgctgct gccgggcgac ccagaacagg tagacggcgg
     4921 cgatcagggc ggcccagggg agccagtgct ccgggaagcg gccctgcgac tgcgccagcg
     4981 ccgcgatcgt cgggatcgcg agcaggacga tgagcccgc
//
LOCUS       DASPJI010000859         6859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_456289, whole genome shotgun sequence.
ACCESSION   DASPJI010000859 DASPJI010000000
VERSION     DASPJI010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6859)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456289"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..944)
                     /locus_tag="VEL74_24130"
     CDS             complement(165..944)
                     /locus_tag="VEL74_24130"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG65694.1"
                     /translation="MLALELDVATTLRETPDIETSSEGYASRFQGPVGDYFLAVQAQM
                     VLDLLQPLQTQRPLRVLDVGGGHAQIAGPLAARGHDVTVFGSDPVCRQRLDRLLPAGS
                     FRFESGDLLRLPFPDRSFDVVVALRLLSHMEQWRELLAELARVAKRAVILDYPDLRSF
                     NLLYGALFKVKKALEGNTRTFLCFRPGEVAAELARHGFGQPAERRQFFVPMVVHRKLG
                     KAGFSRPVERLSATLGLTRALGSPVVLRAVRSGEPGGKPRK"
     gene            complement(944..2464)
                     /locus_tag="VEL74_24135"
     CDS             complement(944..2464)
                     /locus_tag="VEL74_24135"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HYG65695.1"
                     /translation="MSGGLTRDPRRAASSSYDLVVVGGGIHGVAATLEAARRGLSVLL
                     LERDDFGGATTWNSLRIVHGGLRYLQSLDLRRFRESVGERRWLLEHFPDLVEPLPCLM
                     PLYSPPRGGRLRRRTPFRVALALNDRLAPDPALAPGRLLDARETAELCPAVDPEGLSG
                     GALWHDAVAPNSQRLLIEMLRWACGRGAVALSYVEAAELRVEEGRVAGVRALDRASGE
                     RLDVTARAVLNCAGPWARRVAHRFHRDLPALAQPVLAFNLFLDIRADAILRGRTALAV
                     AAPGSGAQTWFLLPWKERILAGTAYVTGAGEAGESGPGDAAVEGFLRELNAALPALGA
                     NRGQVLRVHWGWLPAAAEGSAVPGSRPVIHDHGREGGPAGLVSLSGVKLTTARALAER
                     ALSTLFPGLPRLSPLAPAGRPAADPPLPWEGFARLAEDDWGAARSWLAGFAERQAVVE
                     LDDLLLRRTDWGVIPERAALAERLCRSLLLGRPRGGRAAAPSEDTETLMAAGRGRG"
     gene            complement(2461..3375)
                     /locus_tag="VEL74_24140"
     CDS             complement(2461..3375)
                     /locus_tag="VEL74_24140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65696.1"
                     /translation="MSIPMPQSSAERGPETGPSRAEGFVLTDAHVHVHGCFDSGVFLD
                     AALGNFQRGAGALGLGGSLAFCLLLTEMKGVSFFQRFRDGGAAGGWGGWTFEPTAEPE
                     SLTARRRGTDDRLVLIAGRQIATREGLEVLALCTAEEVPDGLSLGETLDRVRAAGALP
                     VLPWGFGKWWLSRGERVARQIRELPADAELYLGDNAGRPAGALPSRLLRAAEERGIPV
                     LPGSDPLPLETHADRAGSYGFLLSGKLDPDRPAQSLARRVRRVRAAGGRLRPFGRRSG
                     LLQFCRDQATLRAIGRPGHRGSSQGAAR"
     gene            complement(3464..3994)
                     /locus_tag="VEL74_24145"
     CDS             complement(3464..3994)
                     /locus_tag="VEL74_24145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HYG65697.1"
                     /translation="MKLGIVIRRFLMPGFVTTLRAYLKYGCMVSPKAEVEITPHLKIG
                     RKSIVSSFTKLKATEGPLEIGSNVEISNGCVISSHHAGTRIGNDCLIGPNVSIIGNNY
                     RYDRLDVPIRLQEKVSPKGIRIGDNVWLGSGCVVLDGADIGSGTIVTPNSVVSHPLPE
                     NSIAQGNPAKVIFKRR"
     gene            complement(3991..4242)
                     /locus_tag="VEL74_24150"
     CDS             complement(3991..4242)
                     /locus_tag="VEL74_24150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HYG65698.1"
                     /translation="MDTATELDGRIRDVMADILEVEPGDIGDGFGRGDAPSWDSMNHL
                     RMVSALEEAFGVQFTMKEVGEMERFDTIRRLVSGRLQGR"
     gene            complement(4351..4776)
                     /locus_tag="VEL74_24155"
     CDS             complement(4351..4776)
                     /locus_tag="VEL74_24155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65699.1"
                     /translation="MSNASSYADFITEMEGLLAAVTEHAAELPSVETHRAALAAFAEE
                     LKALKLRQDTARAVRQRSTQELKAFKAKGRGLIIRLQGAVKADLGHDNEKLVQFGVAP
                     FRKRVRRTGPFAIKPKAPAQPILTAPAPNAPSGSEEPTT"
     gene            complement(4829..5227)
                     /locus_tag="VEL74_24160"
     CDS             complement(4829..5227)
                     /locus_tag="VEL74_24160"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HYG65700.1"
                     /translation="METPKNSDAETEALQADAMGSDLTRKLIRPRGEKLSRRQREEKL
                     KAERVQEKLLSMPGWRISSDGKGLVKVRVLRDNRTAMAYTAYVNTATEGAGHRAHIIL
                     SGPRVFMTLESRARHGGFTESLLDFAKDLG"
     gene            complement(5619..>6859)
                     /locus_tag="VEL74_24165"
     CDS             complement(5619..>6859)
                     /locus_tag="VEL74_24165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65701.1"
                     /translation="GAELLHLLTCPECREAAAAVLRAGDGVPGVPVPDYDPMFRRLEA
                     NLVHLEEERRRYGEAARLATELCSLPPKERERAVKSLRFARADVLNALLREGEARQEA
                     APAEAVVLGRLAIGLAGRLSWPDAGQKAAARARALILTAGFCRLAGQESLAAVLLRRA
                     VPWLVEPRVRGDYCRTLALLRWQQGRTEEAIALFQRGARVLDEAGLPEEAARCKVPLG
                     LLYTEQGAVRRALEMFLSMELLDPHARPGLAARACLALALGLVDIDMAADARAALRGA
                     HRIYGQIRADLEQVRVYWLEGRVLASLGETEQALALMEQIFAKLFAEGLVAEAAVAGV
                     ELAGLLASLGRAGEIVERLGEVRRACAADLSLQRAVETLAWAASAAGSSLAERASCSA
                     FALRVALRLAGNRVEPPPAA"
BASE COUNT          999 a         2448 c         2467 g          945 t
ORIGIN      
        1 gagcaccacc tcggctcccc agtcgcgaag ctcctgggtc ttcaagccct ctttatagtc
       61 cagggcgacc acctgatggc ccatgtccag cagccgccgg accagggcct taccggtgaa
      121 acccgtaccg ccagtgacca ggatcttcat cgagtctcct cggattactt ccgtggcttt
      181 ccgcccggtt ctccactccg gacggcccgc aggacgaccg gggagcccag cgcccgggtg
      241 agccccaggg tcgcgctcag cctctccacc gggcgggaga accccgcctt gcccagcttg
      301 cggtggacca ccatcggaac gaaaaactgg cgccgctcgg ccggctggcc gaagccgtgg
      361 cgggcgagct cggcggccac ctcgcccggc cggaagcaga gaaaggtacg ggtgttgccc
      421 tcgagcgcct tcttcacttt gaacagggcg ccatagagca gattgaagct gcgcaggtcg
      481 gggtagtcga gaatcacggc gcgcttcgcc acccgggcca gctccgcgag cagctcgcgc
      541 cactgttcca tatgggaaag gaggcgcagc gccaccacca catcgaagga gcggtctggg
      601 aacggcagcc gcagcaggtc gccgctctcg aagcggaacg agcccgcggg cagcagccgg
      661 tccagccgct gccggcagac cggatcgctg ccgaagaccg tcacgtcgtg gccgcgcgcg
      721 gcgagcgggc cggcgatctg ggcatggccg ccgcccacat ccagtacacg gagcgggcgc
      781 tgggtctgta gcggctgcag gaggtccagc accatctgcg cctggaccgc gaggaagtag
      841 tcgccaacgg gcccctggaa acggctggcg tagccctcgg acgaggtctc gatgtcgggc
      901 gtctcgcgca gggtcgtggc cacgtcgagc tccagcgcca gcatcagccc cgtccccggc
      961 cggcggccat cagagtctcg gtgtcctcgc tcggagcggc ggcgcggcca ccccgcgggc
     1021 gtcccaggag cagcgagcgg cagaggcgct ccgccagggc cgcccgctcg gggatcaccc
     1081 cccagtcggt gcgccgcagc agcaggtcgt cgagctccac cacggcctgg cgctcggcga
     1141 accccgccag ccaggagcgc gcggcccccc aatcgtcctc ggcaagccgg gcgaagccct
     1201 cccacggcag cggcggatcg gcggcggggc gccccgccgg agccagtggc gagagccgtg
     1261 gcaggcccgg gaagagggtg gacagggcgc gctccgccag ggctcgcgcc gtggtgagct
     1321 tgacgccgga gaggctcacc aggcccgcgg gtcccccttc gcggccgtga tcgtggatca
     1381 cgggccggga gccgggcacc gctgatccct cggccgccgc cggcaaccag ccccaatgga
     1441 cccgcagcac ctggccgcgg ttcgcgccga gcgccggcag ggctgcgttc agctcgcgca
     1501 ggaagccctc gaccgccgcg tcgccgggtc cggactcccc cgcctcgccg gcgcctgtca
     1561 cgtaggcggt cccggccagg atcctctcct tccaggggag gaggaaccag gtctgcgccc
     1621 cggagcccgg agcggccacg gccagggcgg tcctcccccg caagatggcg tccgcgcgga
     1681 tgtccaggaa gaggttgaag gcgaggaccg gctgcgcgag ggcgggcagg tcgcggtgga
     1741 agcggtgggc gacgcggcgg gcccaggggc cggcgcagtt gagcaccgcg cgggctgtga
     1801 cgtcgagccg ctcgcccgag gcgcggtcca gcgcccgcac ccccgcgacg cgcccctcct
     1861 ccacccgcag ctccgcggcc tccacgtagc tcaaggccac ggcgccgcgg ccgcaggccc
     1921 agcgcagcat ctcgatgagc aggcgctggg agttcggggc cacggcgtcg tgccagagag
     1981 ccccgccgct gaggccctcc gggtccaccg ccggacagag ctccgcggtc tcgcgggcgt
     2041 ccagcagccg gcccggcgcg agggcggggt ccggggcgag acggtcgttc agcgccagcg
     2101 ccacccggaa gggtgtgcgg cggcgcaggc gacctccccg cggaggactg tagagaggca
     2161 tcaggcaggg aagcggctcc accaggtcgg ggaagtgctc cagcagccag cggcgctccc
     2221 ccaccgactc ccggaagcgg cggaggtcca ggctctggag gtagcgcagg ccgccgtgga
     2281 cgatgcgcag gctgttccag gtggtggcgc cgccgaagtc gtcgcgctcg aggagcagga
     2341 cggagaggcc ccgccgcgcc gcctccaggg tcgccgccac gccgtggatc ccgcccccca
     2401 cgaccaccag gtcataggag gaggacgccg cccgccgggg atcccgggtc aagccgccgc
     2461 tcaccgcgcc gctccctggg aggagccccg gtgccccggg cgcccgatag cacggagggt
     2521 ggcctggtcg cggcagaact ggaggaggcc cgagcggcgg ccgaacgggc gcagccgccc
     2581 gccggcggcg cgcacccggc gcacccggcg cgccagggat tgcgccggcc ggtccgggtc
     2641 gagcttgccg gagagcagga agccatagga gccggcccgg tccgcgtggg tctccagggg
     2701 gaggggatcg cttccgggga gcaccgggat gccccgctcc tccgccgcgc gcagcagccg
     2761 ggaagggagc gcgccggccg gccggccggc gttgtcgccc aggtagagct cggcgtccgc
     2821 cggcagctcc cggatctgcc gcgccacccg ctcgccgcgg gagagccacc atttgccgaa
     2881 gccccagggg agtacgggga gcgcgccggc ggcgcggacg cggtccaggg tctcgcccag
     2941 cgaaaggccg tccgggacct cctccgcggt gcagagggcc agcacctcca gcccctcgcg
     3001 ggtggcgatc tgcctccccg cgatcaggac cagccggtcg tccgtgccgc gccggcgcgc
     3061 cgtcagggac tccggctcgg cggtcggctc gaaggtccat cctccccacc cgccggcagc
     3121 gccgccgtca cggaagcgct ggaagaacga gacgcccttc atctcggtga ggagcagaca
     3181 gaacgccagg gacccgccga gccccagcgc tccggcgccg cgctggaagt tgcccagcgc
     3241 cgcgtccagg aagacgccgc tgtcgaagca gccatggacg tggacgtgcg cgtcggtcag
     3301 gacgaaaccc tcggcacggg agggacccgt ctcgggacct ctttcggcgg aggactgtgg
     3361 catcggtatg gacaagcgaa cgtttccccg ctcgaccgca aaggtcggag tgctacgcag
     3421 gggggcctat ctgcaagaag ggttccagac gggccgaggg ccgctaccgc cgtttgaaga
     3481 tgaccttggc cgggttgccc tgggcgatgg agttctccgg cagggggtgc gagaccaccg
     3541 agttcggggt gacgatggtg ccgctgccga tgtcggcgcc gtcgaggacg acgcagccgg
     3601 agccgagcca gacgttgtcg ccgatgcgga tccccttggg cgagaccttc tcctgcaggc
     3661 ggatgggcac gtccagccgg tcgtaacggt agttgttgcc gatgatcgac acgttggggc
     3721 cgatcaggca gtcgttgccg atccgcgtcc cggcgtggtg cgaactgatg acgcagccgt
     3781 tcgagatctc cacgttgctg ccgatctcca gaggcccctc ggtggccttg agcttggtga
     3841 aggagctgac gatggatttc ctgccgatct tgaggtgggg cgtgatctcc acctccgcct
     3901 tggggctgac catgcagccg tacttgagat aggcccggag ggtggtcacg aacccgggca
     3961 tcaggaagcg ccggatgacg attccgagct tcatcggccc tggaggcgtc ccgagaccag
     4021 ccggcggatg gtgtcgaagc gctccatctc gccgacctcc ttcatggtga actggacgcc
     4081 gaacgcctcc tcgagggccg agaccatgcg caggtggttc atggagtccc aggaaggtgc
     4141 gtcgccgcgg ccgaagccgt cgccgatgtc cccgggctcc acctccagaa tgtctgccat
     4201 gacgtcgcgg atgcgaccgt cgagctccgt cgctgtatcc atgcgggcct tctcctcctg
     4261 aaatccgcga tccggcagga gtgcaagaag cgggccggca aagaggggcg tgcctggcgg
     4321 cacgcccccc gaattgctac ggtgctgcgg ctaggtagtg ggctcctcgg agcccgaggg
     4381 cgcgttcgga gccggagccg tgaggatggg ctgcgcgggc gccttgggct tgatggcgaa
     4441 aggtcccgta cggcggaccc gcttgcggaa cggcgccacg ccgaactgca cgagcttttc
     4501 gttgtcatgg cccaggtccg ccttcaccgc gccctggaga cggatgatga ggccgcgccc
     4561 cttggccttg aaggccttga gctcctgggt gctgcgctgg cggacggccc gggcggtgtc
     4621 ctggcggagc ttgagcgcct tgagctcctc cgcgaaggcc gccagggcgg cgcggtgggt
     4681 ttcgacggag ggcaactcgg cggcgtgctc ggtgaccgcc gccagcagcc cctccatctc
     4741 ggtgatgaaa tcggcgtacg acgatgcgtt agacatgatt ctgtttcctc tttcttctca
     4801 tgggtcgggt tgatgtgtcc cccggcacct agccgaggtc cttcgcgaag tccaacaggg
     4861 attcggtgaa tccgccgtgg cgggcccggc tctccagggt catgaacacc cgtgggccgg
     4921 agaggatgat gtgcgcccgg tgtcccgcgc cttccgtggc cgtgttgacg taggcggtgt
     4981 aggccatggc ggtcctgttg tcccggagga cgcggacctt gaccaggccc ttgccgtcgg
     5041 acgagatcct ccaccccggc atggagagca gcttctcctg caccctctcg gccttgagct
     5101 tctcctcccg ctgccgccgg gagagcttct ccccccgcgg gcggatcagc ttccgggtga
     5161 ggtcgctccc catggcgtcc gcctggaggg cctcggtttc cgcatcgctg ttcttcggcg
     5221 tttccataca acgtctcctt gtaagctccg ggccttcatg gcgccggaaa gttgattctc
     5281 tggaggagcc agcgtccaga tcggcgatcc gacttcttac ccctctgtct atgtctacgg
     5341 atgagcccgc ctgagcgtca accgatttcg gagacttttt tcgagaaaat tttacgatct
     5401 tctctttggg cagagcagtg gcgcggcggc gcctcgaagg cgccgaaacg gcgaggtcag
     5461 tcctggggtc ccgcggggtc cggtcagacg gcggccggca ggattcggcg gggtgctgcg
     5521 gtactgcgga aagcgtgctt caggtcaagc ccgttccggc cctgcctcca gccggtttgc
     5581 ccaaatacgg gggatttccc gaacatttgt tcagactttc aggccgcggg agggggttcg
     5641 acccggttcc cggccaggcg cagggccact cgcagggcga aagcggagca agaagcccgt
     5701 tccgccagcg acgagccggc tgccgaggcc gcccaggcga gcgtctcgac ggcccgctgg
     5761 aggctcaggt cggcggcgca ggcgcggcgc acttccccca ggcgctcgac gatctcgccg
     5821 gcacgcccca gagaggcgag caagccggcc agctccacgc cggccacggc cgcctcggcc
     5881 accaggcctt cggcgaagag cttggcgaag atctgttcca tcagggccag ggcctgctcg
     5941 gtctccccga ggctcgccag cacccggccc tccagccaat agacgcgcac ctgttccagg
     6001 tcggcgcgga tctggccgta gatccggtgg gctcctcgca gagccgcgcg ggcgtcggcg
     6061 gccatgtcga tgtccaccag ccccagcgcc agggccaggc aggccctcgc cgccaggccg
     6121 ggccgcgcgt gggggtccag cagctccatg gaaaggaaca tctccagcgc tcgccggacg
     6181 gccccctgct ccgtatagag caggccgagc ggcaccttgc accgggcggc ctcctccggc
     6241 agcccggcct cgtccagcac ccgggcgccg cgctggaaca gggcgatggc ctcctcggtc
     6301 cgtccctgct gccagcgcag cagcgccaac gtccggcagt agtccccccg caccctcggc
     6361 tccacgagcc aggggaccgc ccggcggagc aggacggcgg cgagcgactc ctggccggcc
     6421 aggcggcaga agccggcggt caggatcagc gcccgggcgc gggccgccgc cttctggccg
     6481 gcgtccggcc atgacagccg ccccgccaga ccgatggcga ggcggccgag cacgaccgcc
     6541 tccgcggggg cggcctcctg ccgggcctcg ccctcccgca gcagggcgtt gagcacgtcg
     6601 gcgcgggcga agcgcaggct cttgacggcc ctctcgcgct ccttgggtgg gagggagcac
     6661 agctcggtgg ccagccgcgc cgcctcaccg taccgccggc gctcctcctc caggtggacg
     6721 aggttggcct ccagccgccg gaacatcggg tcgtagtccg gcaccggcac gcccggaacg
     6781 ccgtcaccgg cgcgcagcac ggcggcggcg gcctcgcggc attccgggca ggtgaggaga
     6841 tgcagcagct cggcgccgt
//
LOCUS       DASPJI010000860         3525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_683772, whole genome shotgun sequence.
ACCESSION   DASPJI010000860 DASPJI010000000
VERSION     DASPJI010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683772"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VEL74_24170"
     CDS             <1..1504
                     /locus_tag="VEL74_24170"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HYG65702.1"
                     /translation="LWRYRHRPAGEPAATALRRTLEELARPIVLTSLTTAASFLSFLT
                     SPIRPVWSFGLFTGIGVLFCMFWALVATPALLALRPHAIPAVPERTPASPRSVPRLAR
                     PLLALAVRPRLVLPALALVTAVLALGIPRLVVQDGWLDNFAPDSPLRQASERVDRLFA
                     GTHVLLAVVTFDPPADQVPSIPAASGPLLSGRAVDAVGRFEAGLRQRPEVGGVFGLAS
                     HLSTTAFLWGARLPESREIIDNPSWIYLHARRIGNVRGEARRRELVDDGFRRTVVTLL
                     LEGANYRSTASLIEAVRRLETTELAPVHGRVHFAGDVAVSQAMIPAIVRSQVGSLFLA
                     LAGSLAIVALIFGSVRYGLACVAPTAFAVAWTFGLMGWLGMPLGVATSVFCAVTLGIG
                     VDYGIHFFDRLQAARAAGDSWPGRAAAAQAGPAILVDALAVALGFGLLAVSQVPANRR
                     LGLLVAFGLMSASLLTLLGAGAVLELADRRRSRTDPETRAVPVAAEEAAG"
     gene            1615..2349
                     /locus_tag="VEL74_24175"
     CDS             1615..2349
                     /locus_tag="VEL74_24175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65703.1"
                     /translation="MMSKAFVHVLFAGILLVTLAALPASAQPHIPKGDDYWVTPANGY
                     TFFEFPAGEVESLCGTRPSSGWNHRVKLRGVPAPGSDYDTVVTRLDDAYFDPAGWATT
                     RIQVSQLAFASMSPQSTPCGELNWTVGLFGVQPITYMKIQRRTAMGGIFYADISVRVE
                     LQATNARTGAYVGSLFYTRQLPDPANGTPWSLGTKDEFRAGMTESNNCVDVLREKLAV
                     TDPESDHYYFISDMIAAGQCEKRTAS"
     gene            2647..>3525
                     /locus_tag="VEL74_24180"
     CDS             2647..>3525
                     /locus_tag="VEL74_24180"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYG65704.1"
                     /translation="MDHSFDTLALHAGTAPDPVTGAILTPIYQSTTFVQEGVGRHKGF
                     TYSRSGNPTVAALERRLAALEGADHALCFGTGLAATAALALAELRAGDRAVLSDVLYG
                     GTVRLFQQVLARFGVTVELVDTSNLDLLEAALRVPARLLFLETPANPTLKLTDIAGAV
                     RLARAAGALAVVDNTLLTPVLQRPLDLGADLVLHSTTKFIDGHNATVGGALITRDAAL
                     HERLAFLRNATGSIQSPFNAWLTLQGVKTLPLRMARHSENALRVARALAEHPRLTRLL
                     YPGLESFPQHELALRQM"
BASE COUNT          445 a         1331 c         1181 g          568 t
ORIGIN      
        1 gctctggcgc taccggcacc gcccggcggg ggagccggcg gccacggcgc tccggcgcac
       61 gctggaggag ctggcgcggc ccatcgtcct gacgtcgctc accaccgccg ccagcttcct
      121 ctcgttcctc acctcgccga tccgcccggt ctggagcttc ggcctgttca ccggcatcgg
      181 cgtgctgttc tgcatgttct gggcgctggt ggcgacgccg gcgctcctgg cgctgcggcc
      241 ccacgccatc ccggcggtcc cggagcggac cccggcctct ccgcgcagcg tgccgcgcct
      301 cgcccggccc ctgctcgcgc tggcggtccg gccgcgtctc gtgctgccgg ccctcgccct
      361 ggtcaccgcg gtcctcgccc tgggcatccc gcgtctggtg gtccaggacg gctggctcga
      421 caacttcgcg ccggacagcc cgctgcgcca agcctccgag cgggtcgacc ggctgttcgc
      481 cggcacccac gtgctgctcg cggtggtgac gttcgatccg ccggcggacc aggtgccgtc
      541 cattcccgca gcgagtggcc cgctgctgtc gggccgggcg gtggacgccg tcggccgttt
      601 cgaggcgggt ctgcgccagc ggcccgaggt gggcggggtc ttcggcctgg cgagccatct
      661 ctccaccacg gcctttctct ggggcgcgcg cctcccggag agccgggaga tcatcgacaa
      721 cccctcgtgg atctatctgc acgcgcgccg catcggcaac gtgcgcgggg aggcgcgccg
      781 ccgcgagctg gtggacgacg gcttccggcg caccgtcgtc accctgctgc tcgaaggggc
      841 caactaccgg tcgaccgcct cgctgatcga ggcggtgcgg aggctggaga cgacggagct
      901 cgcgccggtg cacggccgcg tccacttcgc cggcgacgtc gccgtgtcgc aggcgatgat
      961 cccggccatc gtccgctcgc aggtggggtc gctcttcctg gccctggccg ggagcctggc
     1021 gatcgtggcg ctgatcttcg gttcggtgcg ctacggcctc gcctgcgtcg cgccgacggc
     1081 cttcgccgtg gcctggacct tcggcctcat gggatggctg gggatgccgc tgggggtggc
     1141 gacctcggtc ttctgtgccg tgacgctggg gatcggcgtc gactacggca tccacttctt
     1201 cgaccgtctg caggcagccc gcgccgccgg ggactcgtgg ccgggacggg cggcggccgc
     1261 ccaggccggg cccgccatcc tggtggacgc cctcgccgtg gcgctgggtt tcggcctgct
     1321 cgccgtctcc caggtgccgg ccaaccgccg gctcggcctg ctcgtcgcct tcgggctgat
     1381 gagcgccagc ctgctcacgc tgctgggggc gggcgcggtg ctcgagctgg cggaccggcg
     1441 gcggtcccgc accgatcccg aaaccagggc cgtgcccgtc gcggcggagg aggccgccgg
     1501 atgagccgca ccgtgtcggg ctgccgcggc ccgcccccat ccctcgggag ggcggcggcg
     1561 tcccgtagat ctgtgaagct cgtgatttga aacgtttttc aggagaaacc agagatgatg
     1621 tccaaagctt tcgttcacgt attgttcgcc ggaatcctgc tcgttaccct ggcagccctt
     1681 ccggcttcgg cccagcccca catccccaag ggcgacgatt actgggtgac gccggccaac
     1741 ggttacacct ttttcgagtt ccccgcggga gaggtcgagt ctctttgcgg tacccggccg
     1801 tcgagtggct ggaaccatcg ggtcaagctc cgcggcgtgc cggctccggg ctcggactac
     1861 gacaccgtgg tgacccggct ggacgacgcc tatttcgatc ccgccgggtg ggcgacgacc
     1921 cggatccagg tcagccaact cgccttcgcc agcatgagcc cgcagagtac cccttgcgga
     1981 gagctgaact ggaccgtcgg cctcttcggc gtacagccga tcacgtacat gaagatccaa
     2041 cgcaggaccg cgatgggggg aatcttctac gccgacatct ctgtccgcgt ggagctccag
     2101 gcgacgaacg ccaggaccgg tgcctacgtc gggagcctgt tctacacgcg ccagcttccc
     2161 gatccggcca atggcacgcc gtggtcgctc ggcactaagg acgagttccg ggcaggcatg
     2221 accgagagca acaactgcgt cgacgtgctg cgcgagaagc tcgcggtgac cgatcccgaa
     2281 tccgatcact actacttcat ctccgacatg atcgccgccg gtcagtgcga gaagcgcacc
     2341 gccagctgag ccctcgcccg cgggccggcc ttcgccggcc cgcgtcttga cagcccgccc
     2401 gccgccaccc taaagtctcc ggccatggaa ccgcgtgtcg cgatccccgt gtcctgcgag
     2461 ctggtgcgaa tgaccatggt catgcgccgg ccgggcgctg ggctttccac cgcgggatcc
     2521 gccgacttct aatccgaaca gtccaggcgc cctccggcga gaaagaccac cgcccggcct
     2581 cctccggcgg tgaagcgtca tctttctttc aaccggtttc gcggccaacg ccgcgggaga
     2641 acgcccatgg accattcgtt cgataccctc gcccttcacg ccggcaccgc gccggacccg
     2701 gtgacgggcg cgatcctgac gccgatctat cagtccacca ccttcgtcca ggagggggtg
     2761 ggccgccaca aggggttcac ctacagccgt agcggcaacc cgaccgtcgc cgccctcgag
     2821 cggcgcctcg ccgcgctcga aggggcggac cacgccctct gcttcgggac gggcctcgcc
     2881 gccaccgccg ccctcgccct ggccgagctg cgggccggcg accgagcggt cctctcggac
     2941 gtcctctacg gcggcaccgt ccgcctcttc cagcaggtgc tcgcccgctt tggggtgacc
     3001 gtcgagctgg tggacacctc gaacctggat ctcctggagg ccgcgctccg ggtcccagcc
     3061 cgtctgctct tcctggagac accggccaat ccgacgctga agctcaccga tatcgcgggc
     3121 gccgtccggc tggcgcgcgc ggcgggggcc ctggcggtgg tggacaacac cctgctcacc
     3181 ccggtgctcc agcgcccgct cgacctcggg gccgacctcg tcctccactc gaccaccaag
     3241 ttcatcgacg gccacaacgc cacggtgggg ggcgccctga tcacccgcga cgccgccctg
     3301 cacgagcgcc tggccttcct gcgcaacgcc accggctcga tccagtcgcc gttcaacgcc
     3361 tggctcaccc tgcagggggt gaagacgctg ccgctccgca tggcgcgcca ctcggagaac
     3421 gcgctgcgcg tcgcccgggc gctggccgag cacccgcggt tgacgcggct cctctatccg
     3481 gggctcgagt cgttcccgca gcacgagctg gcgctgcggc agatg
//
LOCUS       DASPJI010000861         8954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_568232, whole genome shotgun sequence.
ACCESSION   DASPJI010000861 DASPJI010000000
VERSION     DASPJI010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8954)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8954
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568232"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..156)
                     /gene="moaC"
                     /locus_tag="VEL74_24185"
     CDS             complement(<1..156)
                     /gene="moaC"
                     /locus_tag="VEL74_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011574961.1"
                     /note="MoaC; along with MoaA is involved in conversion of
                     a guanosine derivative into molybdopterin precursor Z;
                     involved in molybdenum cofactor biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HYG65705.1"
                     /translation="MSAQGSFSHLDPQGQAHMVDVSEKPVTRRVAEASCRVLLSAQTL
                     IRLANLPK"
     gene            211..903
                     /locus_tag="VEL74_24190"
     CDS             211..903
                     /locus_tag="VEL74_24190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HYG65706.1"
                     /translation="MRLAIVVPTLNEEETLRRTLPAALALLEAGDEVVVSDGGSRDGT
                     VEVARSLGARVVTGPPGRGGQLNRGAAAIADSTAAGVLVFLHADTTLPPAGARAIREA
                     VAAGADGGAFLLRFDVDRPLQRLGSWLINARTRLVRVPLGDQAQFVTRQAFERLGGFQ
                     EWPILEDLDFAVRLRRSGKAVLVEDFVTTGARRFVEQGAVRTVATNWLIWLLFLLGVS
                     PHRLARLYKHIR"
     gene            complement(1102..1401)
                     /locus_tag="VEL74_24195"
     CDS             complement(1102..1401)
                     /locus_tag="VEL74_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HYG65707.1"
                     /translation="MAGKTDLVNSIADSVEGITRRQATAAFEAVFDSIVAALKAGDTA
                     KVPGFGSFSIGERGARKGRNPATGESITIKASKNVRFRAGKELKEAVNKKKRSRG"
     gene            complement(1499..2887)
                     /locus_tag="VEL74_24200"
     CDS             complement(1499..2887)
                     /locus_tag="VEL74_24200"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HYG65708.1"
                     /translation="MSQTGTGRNRRLTTRRTPQDNVRVAAGWVLDRTLQSLAPAESFL
                     ASALTRFDERDQGLLRELVLGSLRWLRRLDHVITTASSRPFEQIEPVLRSPLRIATYQ
                     LLFLDRIPPHAAVHEAVEQANQLSHRGAASFVNGVLRRIARSPRLEDWPVEERDLVRR
                     LGIEKSHPDFLVARWLSRLGEERTEALLDANNRPKPMQLLAFRDRGGRELLAEALIDE
                     GLEVEPASLSSLGLTVRRGNPLASAAFARGDFYVQDEASQAAALIPPPRPGERVLDAA
                     AAPGGKTFSLVAHEPGVVPALADVSLARALVLRGNLGRLRRGLPLLVADAGVPPFAPG
                     PRFDRVILDLPCTGTGTLRRHPELKWRISEDEIGRLSRQALRLMEGAAPLVAPGGNLV
                     AITCSLEPEENEEVVASFLADHPELSLLPLEGLLEPAMAQGLIGRGGWRMPTGGDHDG
                     FTVHVLQKARAS"
     gene            complement(2967..3941)
                     /gene="fmt"
                     /locus_tag="VEL74_24205"
     CDS             complement(2967..3941)
                     /gene="fmt"
                     /locus_tag="VEL74_24205"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HYG65709.1"
                     /translation="MLPSAAMPAIDRVLFFGTPDFAVPTLAALVAAGRRPLRVVTQPS
                     RPVGRGRGLQDPPVARWAREHGLEVVQPERVRHPEFLEQVAALAPDVAIVVAFGQIFP
                     KALLELPRHGCINLHASLLPRWRGAAPIQASIAAGEPRTGVTTMLMEEGLDTGPILLQ
                     EETEIGPDETAGELFPRLAELGGRLVVRTLGELERGGVVPRPQGDEGVTYAPRLTRDS
                     GKVDWRWTARGIADRLRAYTPWPGLTAELRGEPVKLVRARDLQTVDVPEGVGGEGTFL
                     GLRDGALAVVCGGGTVLGIQELQRPGRKPLRAADFANGERLRAGEVFS"
     gene            complement(4346..5566)
                     /locus_tag="VEL74_24210"
     CDS             complement(4346..5566)
                     /locus_tag="VEL74_24210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65710.1"
                     /translation="MKRNVILAVTAVALLALTSVTPAWAAAPFGSFGGKVGGGNSGAG
                     MMPLFGWALDDDGVERVDILVDGVVVGQASYGRSRPQITRRYPGFPDAAAAGWAYELD
                     TTRFLNGMHTVGARIKSRTGEYVTLAGRRFEFTNVQHNLAPFGKIEFPNRGAEMRGTC
                     SLVDTPRRYSVVSGYAMDVGVQDYDTGVAFVELMIDRQIWSNSRSDCRFSPAEGGLSD
                     CYGLRRLDLEPLFPNVKDAPHSGFRFVLDVGLLIATDTFRPGSHVLTIRSSDHAGQVK
                     DISEIHVTFSCDEFGANENSFGEVGMPRIGNLYHGMIQASGWALDWEGIQHIVIMVDS
                     HPVGTATLGAARPQVSQQYPGYPQSPAPGWSLSFDTRQFANGEHLLEALVVDNAGVET
                     YIGGRRFVISNPVP"
     gene            complement(5688..6689)
                     /locus_tag="VEL74_24215"
     CDS             complement(5688..6689)
                     /locus_tag="VEL74_24215"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HYG65711.1"
                     /translation="MIPAMIQVFTDPRCLEHQSPPGYPERPERLAGIVSHLRSRGWPV
                     TDAGTSPEIREAARAAVLAVHEEAYVERFQRAVERGDSLIDSADNPLSDRTWEASWAA
                     VEVALAAADRAAGSAGSGSGDVTFAAVRPPGHHAERSTAMGFCFFNNVAVAAEHLRRR
                     HGASRVAIFDFDVHHGNGTQHLFEEHADIFYVSTHQFPFYPGTGAAGETGRGPGEGAT
                     LNCPLPAGTGDEGYEETIRGTVLPGLRRFAPDVLLISAGFDAWQDDPLGGMRVTEEGF
                     RRWGEWLRELAEEVCGGRLMAILEGGYDLRALPRLVEAHLEGMSGGTGARSNIEEGR"
     gene            complement(6697..7311)
                     /locus_tag="VEL74_24220"
     CDS             complement(6697..7311)
                     /locus_tag="VEL74_24220"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HYG65712.1"
                     /translation="MGQIATCIVLRDGGRAEWEELVRCHGRELRRRIDRELWRMGGGS
                     RPEQVEELVQEAYCRMLDRGPQPVPEGREALNYLDRIAVSVVVDHLRQEAAAKRGRDV
                     RMEMGGHEDFVSEKTLLRSPDPEQILLLRERRRLFLARCCRHVGQRARRRTLWILALA
                     FFADWRSHEIAGALGGSLSASTIDSIVHRVKHHLRPADRELLRG"
     gene            7494..8930
                     /locus_tag="VEL74_24225"
     CDS             7494..8930
                     /locus_tag="VEL74_24225"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HYG65713.1"
                     /translation="MLRRPLFLLLLACAALTAGAACRPGGPEEKPAQPERPRLAGTAK
                     GWNVLFITVDTLRADRLGAYGYKERATSPRIDELLGGGVRFEQASAQRSSTWPSLASL
                     LTGLYPQAHGVVENGYGFPDDLPTLPKILKGSGYRTGAFLSNMCQANHQGWDSFACSG
                     GQDGKTIRRALEWTQAQGGAGGQGESGKPFFLWVHLFGAHGPYYNGGDHAARVLDPGY
                     EGPVGPKKWMLNRIMSEKVALGPRDIRHLDALYDAAVIGTDRLTASLLDGLRAAGKLD
                     RTLIVFTADHGEELYDHNGYLYHSCSVYQTGLHVPLGITAPGVLPAGARVPQTVEIID
                     VLPTVLGLLAVQAPAEMHGRSLVPYLERPGEGGRGKPAFSEYGSTKIRTVVRDNWKLI
                     QNPDGIDPVCIPDAPPGHYPIAEAELYDLAADPMETKNLAAERPEVVAELSRLIQRRF
                     AGIADRAKGQDLPEELRKELEALGYVAH"
BASE COUNT         1302 a         3209 c         3211 g         1232 t
ORIGIN      
        1 cttcggcagg ttggccaggc ggatcagcgt ctgggcggag aggaggaccc ggcaggaggc
       61 ctcggcgaca cggcgggtca ccggcttctc ggagacgtcc accatgtggg cctggccctg
      121 cgggtcgaga tggctgaagg aaccttgcgc gctcatgaga catcctcaga agtggcggta
      181 tgctagcacc cgcggcacct cccatcctcc atgcggctcg cgatcgtcgt ccccaccctg
      241 aatgaagaag agacgctgcg gcggacgctc cccgcggctc tggccctcct ggaggccgga
      301 gacgaggtgg tggtctccga cggcggcagc cgcgacggca cggtcgaggt ggcccgctcg
      361 ctgggggcgc gcgtggtgac cgggccgccc ggccgcggcg gccagctcaa ccgtggcgcc
      421 gccgccatcg ccgacagcac cgccgccggc gtgctcgtct tcctgcacgc cgacaccacc
      481 ctgccgccgg ccggagcgcg ggccatccgc gaagccgtcg cggcgggtgc cgacggcggc
      541 gccttcctgc tccgcttcga cgtcgaccgc ccgctgcaac gcctgggctc atggctgatc
      601 aacgcccgca cccggctggt acgggtcccg ctcggcgacc aggcccagtt cgtgacgcgg
      661 caggctttcg aaagattggg gggattccag gagtggccga tcctggagga cctggacttc
      721 gccgtgcggc tgcgccggag cgggaaggcg gtcctggtgg aggacttcgt gaccaccggc
      781 gcccgccgct tcgtggagca gggggcggtg cggacggtgg ccaccaactg gctgatctgg
      841 ctgctgttcc tgctcggcgt ctcgccgcac cggctcgccc gcctttataa gcacatccgc
      901 tgacctcgaa gagcctctcc ccggccctgc tcggctcctt cgtgtgcgct ccccctccct
      961 ccctgccacg ctgccagtcg cctgcgccgg gccggggaga ggcccccctg aagcagcaga
     1021 gccccgtggc gttgtccccc cctccgccac agggcccgga cctccggctc tgacctgctg
     1081 gacccctcgg gctcgcctcg ctcagccgcg gctgcgcttc ttcttgttga ccgcctcctt
     1141 cagctccttg ccggcgcgga agcggacgtt cttgctcgcc ttgatggtga tcgactcgcc
     1201 ggtcgcgggg ttgcggccct tgcgcgcgcc gcgctcgccg atcgagaagc tgccgaaacc
     1261 gggaaccttg gcggtgtcgc cggccttgag cgccgcgacg atggagtcga acaccgcttc
     1321 gaaggcggcc gtggcctgcc ggcgggtgat gccctccaca gagtccgcaa tgctattgac
     1381 gaggtcagtc ttaccagcca tctcactcct cctagagttg tttttcgggg cgaaagtgtc
     1441 agcccaaaac gatatgccag ccaagggggg ctgtcaataa cgaaaggagc gtccggagct
     1501 aggaagcacg ggctttctgg agcacatgga cggtgaagcc gtcgtggtcc ccgccggtcg
     1561 gcatgcgcca gccgccgcgg ccgatcagac cctgggccat agccggctcc agaagccctt
     1621 ccagagggag gagcgagagc tcggggtggt ccgccaggaa gctcgccacc acctcctcgt
     1681 tctcctccgg ctccagggag caggtaatcg ccaccaggtt gccgccaggg gcaacgagtg
     1741 gcgccgcgcc ttccatcaga cgcaacgcct ggcgcgacag ccgcccgatc tcgtcctcgc
     1801 tgatccgcca cttgagctcg gggtggcggc gcagcgtccc ggtgccggtg cacggcaggt
     1861 cgaggatcac ccggtcgaag cggggccccg gcgcgaacgg cggcacgccg gcgtcggcca
     1921 ccagcagcgg caggccgcgc cggagccggc cgaggttgcc gcgcagcacc agggcgcggg
     1981 cgagcgacac gtcggccagc gccggcacga cgccgggctc gtgggcgacg agcgagaagg
     2041 tcttgccccc cggcgccgcc gcggcgtcca gcacccgctc acccggccgg ggcggcggga
     2101 tcagggccgc ggcctggctc gcctcgtcct gcacgtagaa gtccccgcgg gcgaaggcgg
     2161 cgctggcgag cggattgccg cgccgcacgg tgagccccag cgacgacagc gacgcgggct
     2221 cgacctccag cccctcgtcg atcagcgcct cggccagcag ctcgcgcccg ccgcggtccc
     2281 ggaaagcgag gagctgcatc ggcttgggac ggttgttggc gtcgagcagc gcctcggtgc
     2341 gctcctcgcc gaggcgcgac agccagcgcg ccaccaggaa gtcgggatgg ctcttctcga
     2401 tccccagccg ccgcaccagg tcccgctcct ccaccggcca gtcctccagc cgcggcgagc
     2461 gggcgatgcg gcgcagcacg ccgttgacga agctggcggc gccgcgatgg ctgagctggt
     2521 tggcctgctc gactgcctcg tgcaccgcgg cgtgcggcgg gatgcggtcg aggaacagga
     2581 gctggtaggt ggcgatgcgc agcggcgagc gcagcaccgg ctcgatctgc tcgaaggggc
     2641 ggctgctggc cgtggtgatc acatgatcca gccggcgcag ccagcgcagg ctcccgagga
     2701 ccagctcgcg cagcagcccc tggtcgcgct cgtcgaaacg cgtgagggcg ctggccagga
     2761 acgactcggc gggagcgagc gattgcagcg tgcggtcgag gacccagccg gcggccacgc
     2821 gcacgttgtc ctgcggcgtg cgccgcgtcg tgagccgccg gttgcggccc gtcccggtct
     2881 gggacacgga tgctgctgga ggctgtcgtt tgaccatgtt ccggctgggc cgttccggcc
     2941 cgccccccgc tcgaggaacg gatgctctag gagaagacct cgcccgcccg caggcgctcc
     3001 ccgttggcga agtcggcggc ccgcaacggc ttgcggccgg ggcgctggag ctcctggatg
     3061 ccgagcaccg tgccgccgcc gcagaccacg gcgagcgcgc catcgcgcag gccgaggaac
     3121 gtcccctccc cgcccacccc ttcggggacg tcaacggtct gcaaatccct ggcccgcacc
     3181 agcttgaccg gctcgccgcg cagctccgcg gtgaggcccg gccagggagt ataggcacgc
     3241 aggcggtccg cgatgccgcg cgccgtccac cgccagtcca ccttgccgct gtcgcgggtg
     3301 aggcgcggcg cgtaggtgac cccttcgtcc ccctgcggcc gcggcacgac gccgccgcgc
     3361 tcgagctcgc cgagggtgcg caccacgagc cggccgccca gctcggcgag gcgcgggaag
     3421 agctccccgg cggtctcgtc cggcccgatc tccgtctctt cctgcaggag gatgggaccg
     3481 gtgtccagcc cctcctccat gagcatggtg gtgacacccg tccgcggctc gcccgcggcg
     3541 atggacgcct ggatgggggc ggcgccgcgc cagcgcggca ggagcgacgc gtggaggttg
     3601 atgcagccgt gccgcggcag ctccagcagc gccttgggaa agatctggcc gaacgccacg
     3661 acgatggcca cgtccggcgc gagcgccgcc acctgctcca ggaactcggg gtgccgcacc
     3721 cgttcgggct ggacgacctc gaggccgtgc tcacgcgccc atcgcgccac cggcggatcc
     3781 tgaagccccc gcccgcgacc caccggccgc gacggctggg tcaccacccg caacggccgc
     3841 cgccccgccg ccaccagggc cgcgagggtc ggcaccgcga aatcgggcgt gccgaagaag
     3901 aggaccctgt cgatggccgg catggcggca gatggtagca ccggcggtgg cctctccgac
     3961 cggcggccgg cacggagcgg gctctgatga gctggatgcg atccggggcg ctcggaagcg
     4021 tcccgggccc cgcggaatcc atcccgttga atcggacgcg atccggagcg ttcgggaagc
     4081 ttccggaaca cctggaatcc atccggttca acaggatgcc atccgcggcg tccgggaaga
     4141 ttcggaagct cgcggaggag atccacggcg tctggacggc ttccagggcg tccggatgaa
     4201 acccacagcg tctgggcgag atcccggtcc ccgtccgcct cctcctttcc cgggaaggcg
     4261 gaagagggaa ccgggggagg gcccaaaaca aaacgccccg ccggagaggc ggggcgtcga
     4321 agggggaccg gagcccgacc ggaggtcagg gcaccgggtt gctgatgacg aagcggcggc
     4381 cgccgatgta ggtctcgacg ccggcgttgt cgaccaccag ggcttcgagg aggtgctcgc
     4441 cgttggcgaa ctggcgcgtg tcgaaggaca ggctccagcc cggcgccggg ctctgcggat
     4501 agcccgggta ctgctggctc acctgcgggc gcgcggcccc cagggtcgcc gtacccaccg
     4561 gatggctgtc caccatgatg acgatgtgct gaatcccctc ccagtcgagc gcccagccgg
     4621 aggcctggat catgccgtgg tagaggttgc cgatccgcgg catgcccacc tcgccgaagg
     4681 agttctcgtt ggcgccgaac tcatcacagg agaaggtgac gtggatctcg ctgatgtcct
     4741 tcacctggcc ggcgtggtcg ctcgaccgga tcgtcagcac gtgcgagccc gggcggaacg
     4801 tgtcggtggc gatcagcagg ccgacgtcca gcacgaagcg gaaaccgctg tgcggcgcgt
     4861 ccttgacgtt ggggaacagg ggctccaggt cgagccggcg caggccgtag cagtccgaca
     4921 gcccgccctc ggccggcgag aagcggcagt cgctgcggct gttggaccag atctgccggt
     4981 cgatcatcag ctccacgaag gcgacgccgg tgtcgtagtc ctggacgccg acgtccatgg
     5041 cgtagccgct gaccacgctg tagcgccgcg gggtgtccac gaggctgcag gtgccccgca
     5101 tctcggcgcc gcggttcggg aactcgatct tgccgaaggg cgccaggttg tgctgcacgt
     5161 tggtgaactc gaaccgccgc ccggccagcg tcacgtactc gcccgtccgg ctcttgatgc
     5221 gggcgccgac ggtgtgcatg ccgttcagga agcgggtcgt atccagctca taggcccagc
     5281 cggcggccgc ggcgtccgga aagccggggt agcgccgcgt gatctgcggc cggctgcggc
     5341 cataggaggc ctgccccacc accacgccgt ccaccaggat gtcgaccctc tccactccgt
     5401 cgtcgtccag cgcccagccg aagaggggca tcatgcccgc cccggagttg ccgccgccga
     5461 ccttgccgcc gaacgagccg aacggcgcgg ccgcccatgc cggggtgacc gaggtcaggg
     5521 ccagcagggc taccgcagtc actgccaaga tgacattgcg cttcatcgct cctacctcca
     5581 cctcgcaacc ccccggttgc cagggagcgc aggcttgtac aggcgaatct ctcaagtaaa
     5641 gcgtgaactt ttccgcagat tctagggacg ggagggagaa aagtcaatca tcgaccttct
     5701 tctatattcg atcgggctcc ggtcccgccc gacattccct cgaggtgggc ctccaccagc
     5761 cgcggcaggg cgcgcaggtc gtagccccct tcgaggatgg ccatcagccg gccgccgcag
     5821 acctcctcgg cgagctcgcg cagccactcc ccccaccgcc ggaagccctc ctcggtgacc
     5881 cgcatgccgc ccagggggtc gtcctgccag gcgtcgaagc cggcggagat cagcagcacg
     5941 tcgggggcga agcggcgcag gccggggagg accgtgccgc ggatggtctc ctcgtagccc
     6001 tcgtcgccgg tgcccgcggg gagagggcag ttgagcgtcg ccccctcgcc ggggccgcgg
     6061 ccggtctcgc cggcggcgcc ggtccccgga tagaagggga actggtgggt gctcacataa
     6121 aagatgtcgg cgtgctcctc gaacaggtgc tgggtgccgt tgccgtggtg gacgtcgaaa
     6181 tcgaagatgg cgacgcggga ggcgccatgg cggcgccgca ggtgctcggc ggccaccgcc
     6241 acgttgttga agaagcagaa gcccatggcg gtggagcgct cggcgtggtg ccccggtggg
     6301 cgcacggcgg cgaaggtgac gtcgccgctt ccggagccag cggagccggc cgcccggtcg
     6361 gcggcggcga gggcgacctc caccgccgcc caggacgcct cccaggtgcg gtcggacagc
     6421 gggttgtccg ccgagtcgat gagggagtcg ccgcgctcca ccgcgcgctg gaaccgctcg
     6481 acgtaggcct cctcgtggac ggcgaggacg gcggcgcggg cggcctcccg gatctccggc
     6541 gaggtgccgg cgtccgtcac cggccagccg cggctccgca ggtggctcac gatgccggcc
     6601 aggcgctccg ggcgctcggg atagccgggg ggggactggt gttcgaggca gcgcggatcg
     6661 gtgaagacct ggatcatggc ggggatcata tccggctcac ccccggagca gctcgcggtc
     6721 cgccggccgg aggtggtgct tgacccggtg gacgatggag tcgatggtgc tggcggagag
     6781 gctgccgccg agggcgccgg cgatctcgtg gctgcgccag tcggcgaaga aggcgagggc
     6841 gaggatccag agggtgcgcc ggcgggcccg ctggccgacg tggcggcagc agcgcgccag
     6901 gaagaggcgg cggcgctcgc gcaggagcag gatctgctcc gggtctggcg agcggaggag
     6961 ggtcttctcg ctgacgaagt cctcgtgccc gcccatctcc atccgcacgt cccggccgcg
     7021 cttggccgcc gcctcctgcc gcaggtggtc gacgacgacg ctcaccgcga tccggtccag
     7081 gtagttgagg gcttcgcgcc cctccgggac cggctgcggc ccccggtcca gcatccggca
     7141 ataggcctcc tgcaccagct cctccacctg ctccggccgg gagcccccgc ccatccgcca
     7201 cagctcccgg tcgatgcgcc gcctcagctc ccgtccgtgg caccggacga gctcctccca
     7261 ttccgcgcga cccccgtcgc gcaagacaat gcacgttgcg atctgtccca tggccccgct
     7321 cctcgaaggg aaaaagggga gcgggagcga cgatcgagag ggactggaga gacggcgagc
     7381 agccacgggc cggaagcccg cacacggaag ggtggacctt ttttaccagt tcgcaacagc
     7441 gtccgcaaga gggggcggtc cgggctacaa tccggccgcc cgaggaccgc tctatgctcc
     7501 gacgccccct cttcttgttg ctactcgcct gcgcggcgtt gactgcgggg gcggcctgcc
     7561 gtcccggcgg gcccgaggag aagccggcgc agccggagcg gccgcggctg gccggcacgg
     7621 ccaaggggtg gaacgtcctc ttcatcacgg tcgacaccct gcgggccgac cggctggggg
     7681 cgtacggcta caaggagcgg gcgaccagcc cgcgcatcga cgagctgctg ggcggcggcg
     7741 tccgcttcga gcaggcgtcg gcgcagcgct cgtccacctg gccgtcgctg gcgtcgctcc
     7801 tcaccgggct ctacccgcag gcgcacgggg tggtggagaa cggctacggc ttcccggacg
     7861 acctgccgac cctgccgaag atcctcaagg gctccggcta ccggaccggc gccttcctga
     7921 gcaacatgtg ccaggccaac caccaggggt gggactcctt cgcctgctcg ggcgggcagg
     7981 acggcaagac gatccgccgc gccctcgaat ggacgcaggc tcaaggcggc gccggcggcc
     8041 aaggagaaag cggcaagcct ttcttcctct gggtccacct cttcggcgcc cacggcccct
     8101 actacaacgg cggcgaccac gcggcgcggg tgctcgatcc gggctacgag ggtccggtgg
     8161 gccccaagaa gtggatgctc aaccgcatca tgtccgagaa ggtcgccctg gggccgcggg
     8221 atatccggca cctcgacgcc ctttacgacg ccgcggtgat cgggaccgac cggctgaccg
     8281 cctcgctgct cgacggcctg cgcgccgccg gcaagctcga caggacgctg atcgtcttca
     8341 ccgccgacca cggcgaggag ctgtacgacc acaacggcta cctctaccac tcctgctcgg
     8401 tctaccagac cggcctgcac gtgccgctgg ggatcacggc gccgggggtc ctccccgccg
     8461 gggcgcgggt gccgcagacg gtggagatca tcgacgtgct gccgacggtg ctggggctcc
     8521 tcgccgtgca ggcgcccgcc gagatgcacg gccggtcgct ggtgccctac ctggagcgcc
     8581 cgggcgaggg gggccggggc aagccggcct tctccgagta cggatcgacg aagatccgca
     8641 ccgtggtgcg ggacaactgg aagctgatcc agaacccgga cggcatcgac ccggtctgca
     8701 tccccgacgc gccgccgggc cactatccga tcgccgaggc cgagctctac gacctggcgg
     8761 ccgacccgat ggagacgaag aacctggcgg cggagcgccc cgaggtggtg gccgagctgt
     8821 cgcgcctcat ccagcgccgc ttcgccggca tcgccgaccg ggcgaagggc caggacctcc
     8881 ccgaagagct caggaaggag ctcgaggcgc tggggtacgt ggcgcactga gcctcgcttg
     8941 acagctcacc gggc
//
LOCUS       DASPJI010000862         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_227814, whole genome shotgun sequence.
ACCESSION   DASPJI010000862 DASPJI010000000
VERSION     DASPJI010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227814"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1505
                     /locus_tag="VEL74_24230"
     CDS             <1..1505
                     /locus_tag="VEL74_24230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65714.1"
                     /translation="FGHASAAMAWDLRLAAGESRDIHLAIPLHPGAETNTDFNAQLAA
                     VERGWREKLDRVEIRLPAAFDQWSRTLRSNVAYILINRDGPGIQPGSRSYERSWIRDG
                     ALTSTALLRLGHAEAAREFLVWFAGHQFDDGKVPCCVDARGADPVPEHDSHGELLHLI
                     AEYVRYTGDRATATSLWSNVQGAVSYIDKIRNQRRTAEYRTPEKLPYFGLLPESISHE
                     GYSDRPVHSYWDDFWGLRGLHDAVELARLLGKDDEARRWTAIRDEFRIDLHASLRRTI
                     ALHKIDYIPGSVERGDFDATSTSIGVAPGGELARLPEKELRQTFERYWQEVLARRDGT
                     KAWDAYTPYELRNARTMLRLGWPERARELIALQMEDRRPAAWNHWAEVVARKPREGRF
                     LGDMPHTWVGSEFIHSFLDLFAYTREEDQALILGAGVPAEWLESGDGISVRSLRTPWG
                     TLDYSMRPEEDGVRVRIGGGVQIPPGGVMIRSPGGDVTVRELPAETVLRR"
     gene            1536..>2614
                     /locus_tag="VEL74_24235"
     CDS             1536..>2614
                     /locus_tag="VEL74_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HYG65715.1"
                     /translation="MTDIRFPDGFLWGASTSAFQVEGSPLADGAGQSIWYRFTHTPGL
                     VADGDTGDVASDHYNRWEDDVRLMRELGLQSYRFGIAWHRVLPQGTGRVNDKGLDFYR
                     RLVDGLLANGIRPMITLFHWDLPAALEDRGGWLNPDIASWFADYARVCYQALDDRVEL
                     WTTLNEPWVVTDGGYLHGALAPGHRSLFETPIASHNLLRAHASAVQAYRAEGKHRVGI
                     VVNLEPKYPASDRPEDLAATARADAYMNRQYLDPIFFGRYPEEMREIFGEAWPDHPAA
                     DLGHIRQSVDFLGINYYTRRVTCDDPAAKPVRAGGVRQTGSTHTETQWEVYPAGLTDT
                     LVWVRDRYGDIPLYVTENGAAFYDPP"
BASE COUNT          401 a          959 c          859 g          395 t
ORIGIN      
        1 cattcggcca cgcctcggcg gccatggcct gggacctgcg cctggcagcc ggcgagtcgc
       61 gggacatcca cctggcgatt cctctccacc ccggcgccga aacgaacacc gatttcaacg
      121 cccagctcgc cgccgtggag cgcgggtggc gcgagaagct cgatcgcgtc gagatccggc
      181 tgcccgccgc gttcgatcaa tggtcccgca ccctgcggag caacgtcgcc tatatcctga
      241 tcaatcgcga cgggccgggc atccagcccg gctcgcgctc ctacgagcgc tcctggatcc
      301 gcgacggcgc cctcacctcg acggcgctgc tgcggctggg gcacgccgag gcggcgcggg
      361 agttcctcgt ctggttcgcc ggccaccagt tcgacgacgg caaggttccg tgctgcgtcg
      421 acgcccgcgg cgcggacccg gtgcccgagc acgacagcca tggcgagctt ctccacctga
      481 tcgccgagta cgtccgctac accggcgacc gcgccaccgc gacgtcgctg tggtcgaacg
      541 tgcagggggc ggtctcgtac atcgacaaga tccggaacca gcggcggacg gcggagtacc
      601 ggacgccgga gaagctcccc tatttcgggc tcctccccga atcgatcagc cacgaggggt
      661 actcggaccg cccggtccac tcctattggg acgacttctg gggcctgcgc ggcttgcacg
      721 acgccgtgga gctcgcgcgc ctgctcggca aggacgacga ggcccgccgc tggaccgcga
      781 tccgcgacga attccggatc gacctccacg cctccctgcg ccgtacgatt gcgctgcaca
      841 aaatcgatta catcccgggc tccgtcgaaa ggggggattt cgacgccacc tcgaccagca
      901 tcggcgtggc ccccggcggc gagctcgcgc ggctcccgga gaaggagctt cgtcagacct
      961 tcgagcgcta ctggcaggag gtcctcgccc ggagagacgg cacgaaggca tgggacgcct
     1021 ataccccgta cgagctgcgc aacgcccgca cgatgctccg cctcggttgg ccggagcggg
     1081 cccgcgagct gatcgccctg cagatggagg accggcggcc ggcggcctgg aaccactggg
     1141 ccgaggtggt cgcccgcaag ccgcgggagg ggcgattcct cggtgacatg ccccatacct
     1201 gggtgggctc cgagttcatc cactccttcc tggacctctt cgcctacacg cgcgaggagg
     1261 accaggcgct gatcctcggc gcgggcgtcc ccgccgaatg gctggagagc ggcgacggga
     1321 tctcggtgcg cagcctgcgc accccctggg ggaccctcga ctattcgatg cggcccgagg
     1381 aggatggcgt gcgggtccgc atcggcgggg gggtgcaaat tccaccgggt ggcgtcatga
     1441 tccgctcgcc cggtggtgat gtcacggtac gcgagctgcc ggcggagacc gttctccggc
     1501 gctgacctga ctgtaacgac acgagaggcc caagaatgac cgacatccgt tttcccgacg
     1561 gcttcctgtg gggtgcgtcc acctcggcct tccaggtgga gggctcgccc ctggccgatg
     1621 gcgccggcca gagcatctgg tatcgcttca cccacacgcc cggcctggtg gccgacggcg
     1681 acaccggcga cgtcgcctcc gatcactaca accgctggga ggacgacgtc cggctgatgc
     1741 gcgagctcgg ccttcagtcc tatcgcttcg gcatcgcctg gcaccgcgtc ctgccgcagg
     1801 ggaccggccg ggtcaacgac aaggggctgg acttctatcg ccggctggtg gacggcctgc
     1861 tcgcgaacgg catccggccg atgatcaccc tctttcactg ggacctgccc gcggcgctgg
     1921 aagaccgggg cggctggctc aacccggata tcgcctcctg gttcgccgac tacgcccggg
     1981 tctgctacca ggcgctcgac gaccgggtgg agctgtggac gacgctcaac gagccctggg
     2041 tggtgaccga cggcggctat ctgcacggcg cgctggcacc tggccaccgc agcctcttcg
     2101 agaccccgat cgcctcgcac aacctgttgc gggcgcacgc ctcggcggtc caggcctacc
     2161 gtgccgaggg caagcaccgc gtcggcatcg tggtgaacct cgagcccaag tacccggcgt
     2221 cggaccggcc cgaggacctc gccgccaccg cccgtgccga cgcctacatg aaccgccagt
     2281 atctcgaccc cattttcttc ggccgttatc ccgaagagat gcgggagatc ttcggtgagg
     2341 cctggcccga tcacccggct gccgacctcg gccacatccg ccagtcggtg gacttcctgg
     2401 ggatcaacta ctacacccgg cgggtgacct gcgacgatcc cgccgccaaa ccggtccgtg
     2461 ccggcggcgt gcggcagacc gggtccaccc acaccgaaac ccagtgggag gtctaccccg
     2521 ccggcctgac cgacaccctc gtctgggtgc gcgatcgcta cggcgacatc cccctctacg
     2581 tcaccgagaa cggcgccgcc ttctacgatc cgcc
//
LOCUS       DASPJI010000863         2401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_55362, whole genome shotgun sequence.
ACCESSION   DASPJI010000863 DASPJI010000000
VERSION     DASPJI010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55362"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2401)
                     /locus_tag="VEL74_24240"
     CDS             complement(<1..>2401)
                     /locus_tag="VEL74_24240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65716.1"
                     /translation="FSLEQGPLLRLALVRLADRSHICWLSVHHIVNDWVSFQTFWREL
                     AVLYDAFSSGRAPRLPELPAQYSDFAAWQREWMSGEVLKDYLDWWQQQLEGFPRFLDL
                     PEDRPRPASGTAKGGRLPVRMDSRLTDELRALAQREGATRFMVVMALCATLFHRLSGQ
                     ERIVVGTLNANRNRPEIEPVFGYFLTQLPFPIDLSGDPPFRELLARVRKVALGAYAHQ
                     ELPFGKLVEAMQPERDLSRQPLVQTLVQLLDAQTAFAGEMSELQLQPFEVEDDNARYD
                     VMIALYEFEHAIGGPLEYNADVFDATTAGRMLDLLYCTTEAAVKDPGARLSELPAFSE
                     AARQQVLVEWNDTWVPALTSAGEPETVVELFAAQAARTPAAVAWSGDGWSLDYARLDE
                     RSNRLARRLRRLGVGPGELVGIYLERTAELPVTLLGTLKSGAAYLPLDLAHPAERRAF
                     MLEEARPVVVLTQEHLLSGLPEGTRTLLLDAGSDPLADESGEPIASGADPEDRAYVIY
                     TSGSTGRPKGVEIPHRTLANFVRAMRRLYTVGEGDAMPAITTIAFDLSVPELYLPMLG
                     GGTTPLLTRETAADGVLLARALDAVHATVLQATPATYRQMLEAGWAGRPEMLLLCGAE
                     AMSRDLADRLLPLGRGLWNFYGPTETTVWSTAWRVEAEGPISVGRPIANTRIYLLDSR
                     FSPVPLGSIGEVCIGGEGVARGYFRRPELTAERFVPDPLSGEPGARLYRTGDLARYRP
                     GGLLEYSGRADHQVKLRGFRIELGEIEARLRQVPGVSEAVVLLREDRPGDQRLTAYLA
                     VG"
BASE COUNT          355 a          840 c          889 g          317 t
ORIGIN      
        1 cgccgaccgc cagataggcg gtgagccgct ggtctcccgg acggtcctcg cgcagcagga
       61 ccaccgcctc ggagaccccc ggcacttggc gcaggcgcgc ctcgatctcc cccagctcga
      121 tgcggaagcc gcgcagcttc acctggtggt cggcacggcc cgaatactcg agcaggccgc
      181 cgggccggta gcgggccagg tcgccggtcc tgtagagccg cgcgcccggc tcgccggaga
      241 gcgggtccgg cacgaagcgc tcggcggtca gctccggccg ccggaagtag ccccgggcca
      301 ccccctcacc gccgatgcac acctcgccga tggagcccag ggggaccgga ctgaaccggc
      361 tgtccagcag atagatgcgg gtgttggcga tgggacggcc gacggagatc ggcccctcgg
      421 cctcgacccg ccaggcggtc gaccagaccg tggtctcggt ggggccgtag aagttccaca
      481 ggccgcgccc cagcggcagc agccggtcgg ccaggtcgcg gctcatcgcc tcggcgccgc
      541 agaggagcag catctcgggc cggccggccc agcccgcctc cagcatctgc cggtaggtgg
      601 ccggcgtcgc ctggagcacg gtcgcgtgga cggcgtcgag ggcccgcgcc aggagcacgc
      661 cgtccgccgc ggtctcacgg gtgaggagcg gcgtcgtgcc gccgcccagc atgggcaggt
      721 acagctcggg caccgacagg tcgaaggcga tggtggtgat cgccggcatg gcatccccct
      781 cgcccacggt gtagagccgc cgcatcgcgc gcacgaagtt ggccagcgtc cggtgcggga
      841 tctcgacccc cttgggccgg ccggtggagc ccgaggtgta gatcacgtag gcccggtcct
      901 cgggatcagc gccggaggca atgggctcgc cgctctcgtc cgccagggga tcgctccccg
      961 cgtcgaggag gagcgtccgg gtgccctccg gcaggccgga cagcaggtgc tcctgggtga
     1021 gcaccaccac cgggcgtgcc tcctccagca tgaaggcgcg ccgctcggcg ggatgggcga
     1081 ggtccagggg cagataggcg gcgcccgact tgagggtccc caggagggtc accggcagct
     1141 cggccgtgcg ctccaggtag atgcccacca gctctccagg gcccacgcca agccggcgga
     1201 ggcggcgggc caggcggttc gagcgctcgt cgagacgggc gtagtcgagg ctccacccgt
     1261 cacctgacca ggcgaccgcc gcgggggtcc gcgccgcctg ggccgcgaac agctcgacca
     1321 cggtctccgg ctcccctgcg gaggtcaatg ccgggaccca ggtgtcgttc cactccacca
     1381 atacctgctg gcgcgccgcc tcgctgaacg ctggcagctc ggagagacgg gcgccggggt
     1441 ccttcacggc ggcctcggtg gtgcagtaca ggaggtccag catcctgccg gccgtggtgg
     1501 cgtcgaagac gtcggcgttg tactccagcg ggccgccgat ggcgtgctcg aactcgtaca
     1561 gggcgatcat cacgtcgtag cgcgcgttgt cgtcctcgac ctcgaacggc tggagctgga
     1621 gctcggacat ttcgccggcg aacgcggtct gcgcgtcgag gagctgcacc agggtctgca
     1681 ccagcggctg gcggctgagg tcccgctccg gctgcatggc ctcgaccagc ttgccgaagg
     1741 ggagctcctg gtgggcatag gcgcccaggg cgaccttgcg gacccgggcg agcaactcgc
     1801 ggaagggcgg atcgcccgac agatcgatgg ggaacgggag ctgggtgagg aagtagccga
     1861 agaccggctc gatctcgggc cggttgcggt tggcgttcaa ggtgccgacc acgatccgct
     1921 cctgccccga gaggcggtgg aacagcgtcg cgcagagggc catgacgacc atgaagcggg
     1981 tggcgccctc gcgttgcgcc agggcgcgca gctcgtcggt gaggcggctg tccatcctta
     2041 ccggtagccg gccgcccttc gccgtgccgc tcgccggccg cggccggtcc tccggcaggt
     2101 cgaggaacct ggggaagcct tcgagctgct gctgccacca atcgaggtag tctttgagca
     2161 cctccccgga catccactcg cgctgccagg ccgcgaagtc ggaatactgg gcggggagct
     2221 ccggcagccg cggcgcgcgg ccggaggaga aggcgtcata gagcaccgcc agctcgcgcc
     2281 agaaggtctg gaagctcacc cagtcgttga cgatgtggtg gaccgagagc cagcagatgt
     2341 gggaccggtc cgccagccgt accagcgcca gccgcagcag cggtccctgc tccagggaga
     2401 a
//
LOCUS       DASPJI010000864         5336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_278267, whole genome shotgun sequence.
ACCESSION   DASPJI010000864 DASPJI010000000
VERSION     DASPJI010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278267"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VEL74_24245"
     CDS             complement(<1..594)
                     /locus_tag="VEL74_24245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine beta-hydroxylase,
                     Fe(II)/alpha-ketoglutarate-dependent"
                     /protein_id="HYG65717.1"
                     /translation="MSTKIELEPDDIRAMDILLDELTSRFDSVEDEELLNSATIYAQE
                     LPRRVRGGLSDFKLREPASGICILSGYEVDGARIGRTPAHWKQRSHPSVTLREEILLV
                     LCGQILGDVIAWATQQDGFLVHDILPIPGHEGEQVGTGSEQTLWWHTEDAFHPLRGDY
                     LGMMCLRNPDHVATTYASVEGLDLPERYLDRLFEPHYT"
     gene            complement(622..1758)
                     /gene="vioD"
                     /locus_tag="VEL74_24250"
     CDS             complement(622..1758)
                     /gene="vioD"
                     /locus_tag="VEL74_24250"
                     /EC_number="4.2.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="capreomycidine synthase"
                     /protein_id="HYG65718.1"
                     /translation="MDVPLALLEDWMRRYYFEVDYDLGSSGVEDYSLADLRRMVGLEW
                     EELDRIVFHDSQTLGGERLRQAIADQWGSGDSGRVMATHGSSEANFLVMNALLRPGDE
                     VVVTDPCYPQLFLIAQALGCRLTKVPLRFELGFQPDIDELCDAITPATRMVVLNFPHN
                     PTGASVDAREQTALIEACARVGAYLVWDNAFGAITYDAPPLPDPTGLYERALSMGTLS
                     KTYGLPGLRVGWCVAAPEVLEEMIRYRDYITLHLSPLVEALASRAIEESERFIGPRRE
                     QCRHNLEVLAGWCEEQRDLVAWARPRGGSCIFPCLLEVPDVEEFCHRLAREHRVMLVP
                     GNCFGHPQHVRLGFGGPAEQFAAGLERLGHFLEVGPREAVLSAP"
     gene            complement(1772..>5336)
                     /locus_tag="VEL74_24255"
     CDS             complement(1772..>5336)
                     /locus_tag="VEL74_24255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65719.1"
                     /translation="DEVTALYPAFGAGLPSPLPELPVQYADFAVWQRAWLAGEELERQ
                     LAFWRAQLSGAPTSLDLPGDRPRPAQASHRGARRGRRLPAALEAGLRRFGREQGTTLF
                     MNLLAAAGALLGRWVGQRDLVLGTPIAGRNRLEVERLIGFFVNTLPLRVDLSDGPDFE
                     HLVRRVREMALAAYEHQDLPFEKLVEELRVERNLGHSPLFQAMLSLPSTGRPRGSREE
                     GRSLTVGTGTAKFELTLEVREEAAGLGLSAEFSTDLFDGVTIDRLLAHLEILVEGMVE
                     EPGRRPEDVAQLAGAERHALLVEWNDTSRPELPGLCLHQPFERRAVLVPEETALVTAA
                     ERLSYGELNRRANRLAHHLRRLGVGPEPRVGVLLERSAGMVVTLLAVLKAGGAYVALD
                     PAYPVERLGLMAWDAGLSVLVTEQRLGVSLPVGAGVRIVRLDSDGPEIAAAPAHDPEA
                     RAVPGNLAYLIYTSGSTGRPKAVGIEHRSAMALVRWAGEVFPEEDLDGVLAATSISFD
                     LSVFELFVPLSWGGKVVLAANALELPELAAAGEVRLINTVPSAMAELAAGTLPPSVRT
                     VNLAGEALKQSLVEQVYRHAGVERVLNLYGPSEDTTYSTFARVERGGRVTIGRPLSGT
                     RVHLLERGGVTPSPLGVAGELCLGGDGLARGYLGRPELTAERFVPDPFAGSAGERLYR
                     TGDLARHLSDGRLEFLGRLDHQVKVRGFRVEPGEIEAALSEHPDIWEAAVVPREEPPG
                     ETRLAAYLVAGDEPPSAAELRSFLLETLPEHMVPSQFFTLGFLPLTPSGKVDRGALAR
                     MGTPSERVASGLDETAMPPRDDLELELVRIWEEVLGTPSVGVTDDFFTLGGHSLLAIR
                     LLDRVARQTGVDLAVSSIFQAPTIERLADLLRRQIDAPHTRPALVPIRPDGSLSPFFC
                     VHAAGGNVFAYVELARRLGPDRPFYGLQTPEPLPGGGSVEELAACYVDAVRAAQPAGP
                     YHLGGWSMGGVVAFEMARQLEAAGDEVRLLALIDSPWPDTGRDDAGPESGADGAELLA
                     AFVRDLTGADLPVPLEELGRLSHEERMARALAFAHRAGALPAAGTGGAAIDRLFAVFA
                     HNIRARRAYRPGPYGGHVDLFLAEDSTAFEEERRWGGVAGGGVEVHSLPGDHYSILKA
                     PAVGTLARRLLTCLETAEAGAAGSATGAHTHHPPDPTGGET"
BASE COUNT          818 a         1912 c         1889 g          717 t
ORIGIN      
        1 cgtatagtgg ggctcgaaga ggcggtccag atatctctcg ggcaggtcga gcccttcgac
       61 ggaggcatag gtggtggcca catggtcggg gttcctcagg cacatcattc cgaggtagtc
      121 gccgcgcaag ggatggaagg cgtcctcagt gtgccaccag agagtctgct cgctcccggt
      181 gcccacctgt tccccctcgt ggcccggaat cggaaggatg tcgtgcacga ggaagccgtc
      241 ctgctgggta gcccaggcga tcacgtcgcc cagaatctgg ccgcagagca cgagcagaat
      301 ctcctcgcgc agggtgacgg acgggtggga cctctgcttc cagtgcgccg gcgtcctgcc
      361 gatccgggcg ccatctacct cgtaccccga caggatgcag atcccggagg ccggctcccg
      421 cagcttgaaa tcgctcaggc cgccgcggac ccgccgcggc agctcctggg cgtagatggt
      481 ggccgagttc aggagctcct cgtcttcgac gctgtcgaag cgcgaggtca gctcgtccag
      541 aaggatgtcc atggcccgga tgtcgtcggg ctcgagctcg atcttggtgc tcataacgcg
      601 tttctcccaa atctcaacaa ttcaaggggc actgaggacc gcctcgcgcg gaccgacttc
      661 caggaagtgg cccagccgct cgaggccggc ggcgaactgc tcggcgggcc cgccgaagcc
      721 cagccgcacg tgttggggat gcccgaagca gttgccgggc accagcatca cccggtgctc
      781 gcgggcgagg cgatgacaga actcctcgac gtcaggtacc tcgaggagac atggaaagat
      841 gcacgagccg ccgcgcgggc gggcccaggc gaccaggtcc ctttgctcct cgcaccagcc
      901 cgccagcacc tccaggttgt gtcggcactg ctcccggcgg gggccgatga accgctccga
      961 ctcctcgatg gcacggctgg ccagggcctc gaccagcggc gagaggtgga gggtgatgta
     1021 gtcgcggtag cggatcatct cctcgagcac ctcgggggcg gccacgcacc agccgacccg
     1081 caggcccggc aggccgtagg tcttggacag cgtccccatg gagagggcgc gctcgtagag
     1141 cccagtagga tcgggaagcg gtggcgcgtc ataggtgatg gcgccgaagg cgttgtccca
     1201 gacgaggtaa gcccctaccc gggcacaggc ctcgatcagg gccgtctgct cccgcgcgtc
     1261 gaccgaggcg ccggttgggt tgtgggggaa gttgagcacc accatgcggg tcgccggggt
     1321 gatggcgtcg cagagctcgt cgatgtccgg ctggaagccc agctcgaaac ggagcggcac
     1381 cttggtcagg cggcagccca gggcctgggc gatgagaaag agctggggat agcagggatc
     1441 ggtaaccacc acctcgtcgc ccgggcgcag cagcgcgttc atcaccagga agttggcctc
     1501 gctcgagccg tgagtcgcca tcacccgtcc gctgtcgccg ctcccccact ggtcggctat
     1561 ggcctggcgc aggcgctctc ctcccagcgt ctggctgtcg tggaagacga tccggtccag
     1621 ctcctcccat tcgaggccga ccatgcggcg cagatcggcg agggagtagt cctcgacccc
     1681 actgctgccg aggtcgtagt cgacctcgaa gtagtaccgt cgcatccagt cttccagcag
     1741 ggctagaggt acgtccatga agctcttctc ctcaagtctc accacccgtg gggtccgggg
     1801 ggtggtgcgt gtgggctccg gtggctgaac cggcggcgcc ggcctcggcc gtttccaggc
     1861 aggtcaggag gcgccgggcc agtgtgccga cggccggcgc cttcaggatc gaatagtgat
     1921 cgccgggcag gctgtgcact tcgacgccgc cgccggccac ccctccccac cgccgctcct
     1981 cctcgaaggc ggtggagtcc tcggcgagga agaggtcgac atgcccgccg tagggacccg
     2041 gccggtaggc gcggcgggcc cggatgttgt gggcgaagac ggcgaagagg cggtcgatcg
     2101 ccgcgccacc ggtgccggcg gcgggcaggg cgccggcgcg gtgcgcgaac gccagggcgc
     2161 gcgccattct ctcctcgtgg ctcaggcgcc ccagctcctc caaggggacc ggcaggtcgg
     2221 caccggttag gtcacgaacg aaggccgcca gcagctcggc cccgtcggct ccgctctccg
     2281 gcccggcatc gtcccgcccg gtgtcgggcc aggggctgtc gatcagcgcc aggagccgga
     2341 cctcgtcgcc ggccgcctcg agctggcggg ccatctcgaa ggcgaccacg ccgcccatcg
     2401 accatcctcc caggtgatag gggccggccg gctgcgcggc gcggacggcg tcgacgtagc
     2461 aggccgctag ctcctcgacg gagccgccgc cggggagggg ctcgggcgtc tgcaggccgt
     2521 agaacggacg atcgggaccg agcctgcgcg ccagctcgac ataggcgaag acgttgccgc
     2581 ccgcggcatg gacgcagaag aagggggaca gggatccgtc cgggcggatt ggcaccaggg
     2641 ccggacgcgt gtgcggcgcg tcgatctggc ggcgcagcag gtcggcgagc cgctcgatcg
     2701 tcggcgcctg gaagatcgac gagacggcga gatccacccc cgtctggcgc gccacccggt
     2761 cgaggaggcg gatcgccagc aacgagtggc cgccaagggt gaagaagtcg tcggtgacgc
     2821 cgaccgaggg ggttcccagc acctcttccc agatccgcac cagctcgagc tccaggtcgt
     2881 cccgaggcgg catggcggtc tcgtccagtc cgctggccac ccgctccgac ggcgtgccca
     2941 tgcgagccag agcaccccgg tcgaccttac cgctgggggt cagggggagg aagccgaggg
     3001 tgaagaactg cgacggcacc atgtgctcgg gcagcgtctc gaggaggaac gagcggagct
     3061 cggcggccga agggggctcg tcgccggcca ccaggtaggc ggccaagcgc gtctccccag
     3121 gaggctcttc gcgcggcacc acggcggcct cccagatgtc cggatgctcg ctgagcgccg
     3181 cctcgatctc gccgggctcc accctgaagc cgcgcacctt gacctggtgg tcgaggcggc
     3241 ccaggaactc caggcgaccg tcagacaggt gacgggcgag atcgccggtg cggtagagcc
     3301 gctccccggc gctgccggcg aaggggtcgg gcacgaagcg ctcggccgtc agctccggcc
     3361 gtcccagata gcctcgggcg aggccgtcgc cacccaggca gagctcgccc gccaccccga
     3421 gaggtgacgg cgtcactccg ccgcgctcca gcagatggac ccgggtgccc gagaggggcc
     3481 ggccgatggt caccctcccg ccccgctcca cccgcgcgaa ggtcgagtag gtcgtgtcct
     3541 ccgacgggcc gtataggttc agcaccctct cgactcccgc gtgacggtag acctgctcca
     3601 ccagggactg cttgagcgcc tcccccgcca ggttgaccgt ccgcaccgac ggcggcaggg
     3661 tgccggcagc cagctccgcc atggccgagg ggacggtgtt gatcagccgc acctcgcccg
     3721 ccgcggccag ctccggcagc tccagggcgt tggccgccag caccaccttg ccgccccagg
     3781 agagggggac gaagagctcg aagaccgaca ggtcgaagct gatagaggtg gccgccagga
     3841 cgccgtccag atcctcttcc gggaacacct cgccggccca gcgcaccagg gccatggcgc
     3901 tgcggtgctc gatgcccacc gccttgggcc ggccggtcga gcccgacgtg tagatgaggt
     3961 aggccaggtt acccggcacc gcccgagcct ccgggtcgtg agccggcgcg gcggcgatct
     4021 ccgggccgtc gctgtcgagc cgcacgatcc ggacgccggc ccctaccggc agggagacgc
     4081 cgagccgctg ctcggtcacg aggacggaca ggccggcgtc ccaggccatg agcccgagcc
     4141 gctccacggg gtaggcgggg tccagggcca cataggcgcc gccggccttt aggaccgcca
     4201 gcagagtgac caccatgccg gcggagcgct cgaggagcac gccgacgcgc ggctccggcc
     4261 cgacgccgag ccgccgcagg tggtgggcca gccggttggc ccggcggttc agctcgccat
     4321 agctcagccg ctccgccgcc gtcaccagcg ccgtctcctc cggcacgaga acggcccggc
     4381 gctcgaacgg ctggtgcagg cagagacccg gaagctccgg ccgcgaggtg tcgttccact
     4441 ccaccagcag ggcgtggcgc tcggccccag cgagctgcgc cacgtcctcc ggccgccggc
     4501 cgggctcctc caccatcccc tccaccagga tctccaggtg ggccagcagc cggtcgatgg
     4561 tgacgccgtc gaaaaggtcg gtgctgaatt cggcgctcag ccccaggccg gccgcttcct
     4621 ctcggacctc caacgtcagc tcgaacttgg ccgtgccggt ccccaccgtc agggagcggc
     4681 cctcctctcg cgagccgcgc ggccggccgg tgctcggcag cgacagcatc gcctggaaaa
     4741 gaggcgagtg cccgaggttg cgctccaccc gcagctcctc caccagcttc tcgaacggca
     4801 ggtcctggtg ctcgtacgcg gccagcgcca tctcccgtac ccggcgcacc aggtgctcga
     4861 agtcgggccc gtccgagagg tccacccgca gcggcagggt gttgacgaag aagccgatga
     4921 gccgctccac ctccagccgg ttgcggccgg cgatgggggt acccagtacc agatcgcgct
     4981 gccccaccca gcggcccagc agggccccgg ccgccgccag caggttcatg aacagcgtcg
     5041 ttccctgctc gcgcccgaac cgcctcaggc cggcttcgag agctgccggc agccgccgcc
     5101 cgcgccgcgc gccgcgatgg ctcgcctggg ccgggcgcgg ccggtctccc ggcagatcca
     5161 gcgacgtggg ggcgccggat agctgcgcgc gccagaaggc gagctgccgt tccagctcct
     5221 cgcccgccag ccaggcccgc tgccagacgg cgaaatcggc gtactggacc ggcagctcgg
     5281 gcagcggcga cggcaggcca gcgccgaagg ccggatagag cgccgtcacc tcgtcc
//
LOCUS       DASPJI010000865         6810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_509414, whole genome shotgun sequence.
ACCESSION   DASPJI010000865 DASPJI010000000
VERSION     DASPJI010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509414"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VEL74_24260"
     CDS             <1..993
                     /locus_tag="VEL74_24260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65720.1"
                     /translation="LQNFVYLGDFGAMVRTWMSGDGALLGPQLGTLLALGALFAAALA
                     LLAWVYGGGALRLLRGGGLAVPWAFLLGALAFAIPWNLNEADFYFQITFPTVVMMASA
                     AMTAMVVPVRQRVLEAALVILVAVTVLAGWAIPRKSYPLYRYTTELRAGLGERDLLVH
                     WSHWAGGPSLLFMDLPGVERLYPDKLFYKASDPEAVFPRLAGTLDRRLAAGGRVYLFH
                     LLDRQLWNAPWPRLRRRGLDPERWERFFHERYTVIDRGQVAEMPCWELRPKPGSALAP
                     APAQRIGLPPGDTQSRNSHAHDPNDTQVPQVHPRHGISRTDRRGAGQLPAPALR"
     gene            884..1861
                     /locus_tag="VEL74_24265"
     CDS             884..1861
                     /locus_tag="VEL74_24265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HYG65721.1"
                     /translation="MPTTRTTRKSRKSILVTGSLGQIGAELVSSLRQRYGEEAVIASD
                     IRMPPRGTDGPFEFVDCTNLRSLQEVIHRHHVGTIYHLAALLSAIAESKPQVAWDINM
                     GGLYRVLEVARENRCAVFFPSSIGAFGPGTPQDDTPQDTVQRPTTMYGVTKVAGELLC
                     DYYFHRFGVDARGVRFPGLISYGAPPGGGTTDYAVEIFYEAIRHRHYTCFLGPDTRLD
                     MMYMPDAVKAVIQLMEVDATRLTHRNAFNVTAMNFTPEELAAEIRKHIPDFVIDYHVD
                     PVRQAIADSWPNSLDDSAARNEWGWSPDYDLTAMVRDMLDNLGRKLKRS"
     gene            1882..3114
                     /locus_tag="VEL74_24270"
     CDS             1882..3114
                     /locus_tag="VEL74_24270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HYG65722.1"
                     /translation="MPHERFIAALSTELDTLRERGTLKGDESVITRVLPAEGERGPRY
                     LLDGHGDRPFLKMNSNNYLGMALRREVIEAEEETTAAFGAGPGAVRFISGTYRQHVEL
                     EERLATFHGREASMIFSSAYATTLGVLVPLIHAETVVISDELNHNCIINAMRLSRPKD
                     KQVYRHLDLAELDAKLAAAAGTCRRVIVVTDGVFSMRGDWAPLPEIQAVIDRYDEKFP
                     ENVLLVVDDSHGVGALGRTGRGTEELTGAPPADILIGTLGKAFGVNGGYVTGRRRLIE
                     YLRETAATYIYSNPITPGEAAAAARALAILDSPEGLKLLDHLRAMTSRFETGIVGLGY
                     ETIPGEHPVVPLMVRDTPKTNALVRHLKENGILATGLKYPVVPRGDEEIRFQISADHT
                     PYDIDFTLKALESFPGRG"
     gene            complement(3345..4199)
                     /locus_tag="VEL74_24275"
     CDS             complement(3345..4199)
                     /locus_tag="VEL74_24275"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HYG65723.1"
                     /translation="MPVEGTAAMREEIERLFGAPPAEYGPEHLGLFERFKAALNRGEV
                     RAAEPDPSAPTGWRVNAWVKQGILVGFRMGRIVDMTAGGLPFFDKSTYPPRPLDAAAG
                     VRVVPGGSAIRDGCFLGQGVICMPPMYINVGAWVGDGTLVDSHALVGSCAQVGERVHL
                     SAAAQLGGVLEPVGALPVIVEDDVLVGGNCGVYEGTVVKRRAVLAAGTILTRSTPVYD
                     LVRETVHRAGGDTPLIVPEGAVVVPGSRAVAKGAGKDWGLSLYTPVIVKYRDAGTDER
                     VQLEDWLR"
     gene            complement(4189..5100)
                     /gene="dapA"
                     /locus_tag="VEL74_24280"
     CDS             complement(4189..5100)
                     /gene="dapA"
                     /locus_tag="VEL74_24280"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HYG65724.1"
                     /translation="MFQGCGTALVTPFRQDLSVDEECLRRLVRRQIEAGIHFLVPCGT
                     TGENPTLTREEHLRVVEITREEAAGKVPVLAGCGGNDTRSVVELARSLDGMGVDGLLV
                     VTPYYNKPTPEGLVEHYRAVSRASRLPIIVYNVPGRTAVNVDAATLAQLAEIDTVVGV
                     KEASGNISQMAQIFQVVPEDFAVLSGDDAITLPLIALGGSGVISVASNEVPFAMSRMV
                     SLCLEGDFPAAQKMHRQLHALMEINFVESNPGPVKAALAMMGQLEPVFRLPAVSPRPE
                     SWKKIENVLQELGLIPAGAEERAALAG"
     gene            complement(5120..5824)
                     /locus_tag="VEL74_24285"
     CDS             complement(5120..5824)
                     /locus_tag="VEL74_24285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HYG65725.1"
                     /translation="MTRLALVGHGKMGRLVEQLAPEHGFEVVARLTGSENAAGKSIDA
                     EHLGGAEVAIDFSTAAAVAANAERLAALGIPLVVGTTGWHDDLPRVRAAVERHGSGLL
                     HGANFSVGVQVFYRLAEAAARLLSGEDGYDAWLYEIHHRHKKDAPSGTLKEIQRVMER
                     AGYDRRVDVASNRAGAIPGTHRIGFDSEADTITLEHSARSRAGFARGALRAARWILGR
                     RGFYEFSEVWEEIVRE"
     gene            complement(5821..>6810)
                     /locus_tag="VEL74_24290"
     CDS             complement(5821..>6810)
                     /locus_tag="VEL74_24290"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65726.1"
                     /translation="AALTHHGVAAEWVDCRRVIVTDAEFTRARPLYGPTDARLRGSVP
                     AVVERGAVAVLGGYVGSTADGLTTTLGKEGSDFTAAVVGAALGAEEIQIWTDVEGLLT
                     ADPRVVPGARRLRCLSFGEALELACSGAKKPHPGTLGPASRAAVPIRILSSLHSPHDG
                     EPGEGTIIGPRAPQGPPRVRSITCRGNAHRLRVAANGPWARNGFLSWALDACERFRPA
                     LQVLSVADGEVELALDRNERLNEIRSDLGRTSQVEVSPGTGVVSLVSQDLGTHPELVA
                     RVLEAAREYEPRLITQGAACPVVRCLVEEARLDEVVALLHERLFAGEPPGAIG"
BASE COUNT         1017 a         2522 c         2304 g          967 t
ORIGIN      
        1 ctccagaact tcgtctacct gggggacttc ggcgccatgg tgcggacgtg gatgagcggc
       61 gacggcgccc tgctcgggcc gcagctcggc accctgctcg ccctgggggc gctcttcgcg
      121 gcggcgctgg cgctgctggc ctgggtctac ggcggaggag cgctccgcct gctgcgcggc
      181 ggcggcctgg cggtgccctg ggcgttcctg ctgggcgccc tggcgttcgc catcccctgg
      241 aacctgaacg aggcggactt ctacttccag atcaccttcc ccacggtggt gatgatggcg
      301 tcggcggcaa tgacggccat ggtggtgccg gtccgccagc gggtcctgga ggcggcgctg
      361 gtcatcctgg tcgcggtcac cgtgctggcc ggctgggcca tcccccggaa gagctatccc
      421 ctctatcgct acaccaccga gctgcgcgcc gggctcggcg agcgcgacct gctggtgcac
      481 tggagccact gggcgggggg cccgtccctc ctcttcatgg acctgccggg ggtggagcgg
      541 ctctacccgg acaagctctt ctacaaggcg agcgatcccg aggcggtctt cccccggctc
      601 gccggcaccc tggaccgcag gctcgccgcg ggcggtcgcg tctacctctt ccatctcctg
      661 gaccgtcagc tctggaacgc gccttggccg cgcctgcgcc gccgcgggct cgatcccgag
      721 cggtgggagc gcttcttcca cgagcgctac accgtgatcg accgtggcca ggtcgccgag
      781 atgccctgct gggagctgcg gcccaagccg gggagcgcac ttgccccggc acccgcccag
      841 cgcataggat tgccgcccgg cgacactcaa agccggaact cccatgccca cgacccgaac
      901 gacacgcaag tcccgcaagt ccatcctcgt cacgggatct ctcggacaga tcggcgcgga
      961 gctggtcagc tccctgcgcc agcgctacgg tgaagaggcc gtcatcgcct ccgacatccg
     1021 gatgccgccc cgtggcaccg acgggccctt cgagttcgtc gactgtacca acctccggtc
     1081 cctccaggag gtgatccacc gccatcacgt ggggaccatc taccacctgg cggccctcct
     1141 ctcggcgatc gccgagagca agccacaggt ggcctgggac atcaacatgg gcgggctcta
     1201 ccgcgtcctc gaggtcgcca gggagaaccg ctgcgccgtc tttttcccga gctccatcgg
     1261 cgccttcggc cccggcacgc cccaggacga cacgccgcag gacaccgtcc agcggcccac
     1321 caccatgtac ggcgtcacca aagtcgccgg cgagctgctg tgcgactact acttccatcg
     1381 cttcggcgtc gatgcccgcg gcgtgcgctt cccgggcctc atctcctatg gcgccccgcc
     1441 cggcggcggc accaccgact acgcggtgga gatcttctac gaggcgatcc ggcaccggca
     1501 ctacacctgc ttcctggggc ccgacacgcg cctcgacatg atgtacatgc cggacgcggt
     1561 caaggcggtc atccagctca tggaggtcga cgctacgcgc ctgacgcacc gaaacgcctt
     1621 caacgtcacg gccatgaact tcacgccgga ggagctggcg gccgagatcc gcaagcacat
     1681 cccggatttc gtgatcgatt atcacgtcga cccggtgcgg caggccatcg ccgactcgtg
     1741 gcccaactcg ctggacgaca gcgccgcccg caacgagtgg ggctggtccc ccgattacga
     1801 cctgacggcc atggtccggg acatgctcga caatttgggc cggaagctca agcggagctg
     1861 agcccaggag aacacgcttc catgccacac gagcgcttca tcgcggccct ttccaccgag
     1921 ctcgacaccc tgcgcgagcg ggggacgctc aagggcgacg agtccgtcat cacccgcgtc
     1981 ctgcccgccg aaggcgagcg cggcccccgc tatctcttag acgggcacgg cgaccggccg
     2041 ttcctcaaga tgaactccaa caactacctg ggcatggccc tgcggcgcga ggtcatcgaa
     2101 gccgaggagg agaccaccgc cgccttcggc gcgggcccgg gggccgtgcg tttcatcagc
     2161 ggcacctatc gccagcacgt cgagctcgag gagcgcctcg ccaccttcca cggccgcgag
     2221 gcgtcgatga tcttcagctc cgcctatgcc accaccctcg gcgtgctcgt gcccctgatc
     2281 cacgccgaga cggtggtgat cagcgacgag ctcaaccaca actgcatcat caacgcgatg
     2341 cgcctgtccc ggcccaagga caagcaggtc taccgtcacc tggacctggc ggagctggac
     2401 gccaagctcg ccgctgcggc cggcacctgc cggcgtgtca tcgtcgtcac cgacggcgtg
     2461 ttcagcatgc gcggcgactg ggcgccgctg ccggagatcc aggccgtcat cgaccgctac
     2521 gatgaaaagt tcccggagaa cgtcctgctg gtagtggacg actcgcacgg cgtcggcgcc
     2581 ctggggcgga ccggccgcgg caccgaggag ctcaccggcg ccccgccggc cgacatcctc
     2641 atcggcacgc tgggcaaggc cttcggcgtc aacggcggct acgtcaccgg ccggcggcgg
     2701 ctgatcgaat acctgcggga gaccgcggcc acctatatct actcgaaccc catcaccccc
     2761 ggtgaggccg ccgcggcggc gcgggcgctc gccatcctcg acagcccgga ggggctgaag
     2821 ctgctcgatc acctgcgggc catgacctcc cgcttcgaga cgggcatcgt cggcctgggc
     2881 tacgagacga tccccggcga gcacccggtg gtgccgttga tggtgcgcga cacgccgaag
     2941 accaacgcgc tggtgcggca cctcaaggag aacggcatcc tggcgaccgg cctcaagtat
     3001 ccggtggtgc cgcgcggcga cgaagagatc cgcttccaga tctccgccga ccacaccccg
     3061 tacgacatcg atttcacctt gaaggcactg gagagcttcc cggggcgggg atagaggcgt
     3121 acaatgggat ttgtccgggg gcctcttcgc gggggcgcct ggagcgcggc ccggggcgag
     3181 cgagccctgg acggcgtgtc gagccctcca aaacccggag gagcggcggc tcgctccaag
     3241 gctggcggac cgaggtcgcg ccgtccaacg gagcacctcg ccggtttcga gatcgaaagc
     3301 cagtttcgag tcgaaccgcc gcccccccgg ggcttacagt ttccctatcg caaccagtct
     3361 tcgagctgga cccgctcgtc ggtgccggcg tcccggtatt tcacgatcac cggcgtatag
     3421 agggagaggc cccagtcctt gccggccccc ttggcgacgg ccctggagcc ggggaccacc
     3481 accgccccct cggggacgat cagcggcgtg tcgccgccgg cgcggtgcac ggtctcccgc
     3541 accaggtcgt agaccggcgt cgagcgggtg aggatggtgc ccgcggcgag caccgcccgg
     3601 cgcttcacca ccgtgccctc gtagacgccg cagttgccgc ccaccagcac gtcgtcctcg
     3661 acgatcaccg gcagcgcccc caccggctcg agcacgccgc cgagctgcgc cgccgccgag
     3721 aggtgcaccc gctcgcccac ctgcgcgcag ctccccacca gggcgtgcga gtcgaccagc
     3781 gtcccgtccc ccacccaggc gccgacgttg atgtacatcg gcggcatgca gatcaccccc
     3841 tggccgagga agcagccgtc gcggatcgcc gagccgccgg gcaccacccg cacgccggcc
     3901 gcggcgtcga gcggccgcgg cggataggtc gacttgtcga agaaggggag gccgcccgcc
     3961 gtcatgtcga cgatgcgccc catgcggaag ccgacgagga tcccctgctt gacccaggcg
     4021 ttcacccgcc atcccgtcgg cgcggaggga tcgggctcgg cggcccgcac ctcgccgcgg
     4081 ttgagcgccg ccttgaagcg ctcgaagagg ccgaggtgct ccggcccgta ctcggcgggc
     4141 ggagcaccga agaggcgctc gatctcctcg cgcatcgcgg cggtgccctc aaccggcaag
     4201 agcggccctc tcctcggcgc ccgccgggat caatcccagc tcctgcagca cgttctcgat
     4261 cttcttccag ctctcgggcc gcggcgacac cgccggcagc ctgaagaccg gctcgagctg
     4321 gcccatcatc gccagcgccg ccttgaccgg gccggggttg gactcgacga agttgatctc
     4381 catcagcgcg tggagctggc ggtgcatctt ctgggccgcc gggaagtccc cttccaggca
     4441 gagcgacacc atccgcgaca tggcgaaggg cacctcgttc gaggccaccg agatcacccc
     4501 ggagccgccg agggcgatca gggggagggt gatggcgtcg tcgcctgaca gcacggcgaa
     4561 gtcctcgggc accacctgga agatctgcgc catctgcgaa atgttgcctg acgcctcctt
     4621 gacccccacc accgtatcga tctccgccag ctgcgccaga gtcgccgcgt cgacgttgac
     4681 cgccgtgcgg cccggcacgt tgtagacgat gatcggcagc cggctcgccc gcgacaccgc
     4741 ccggtagtgc tcgaccaggc cctccggcgt cggcttgttg tagtaggggg tgacgaccag
     4801 caggccgtcg acgcccatcc cgtcgagcga ccgggcgagc tccacgacgc tccgcgtatc
     4861 gttgccgccg cagccggcga ggaccgggac cttgccggcc gcctcctccc gggtgatctc
     4921 caccacccgc aggtgctcct cgcgggtgag cgtcgggttc tctccggtcg tcccgcaggg
     4981 gaccaggaaa tggatacccg cctcgatctg gcggcgcacc agacggcgca ggcactcctc
     5041 gtccaccgac aggtcctgcc ggaacggagt gaccagagct gtcccacaac cttgaaacat
     5101 gacggcctcc cgtccggggt cattcccgga cgatctcttc ccagacctcg ctgaattcgt
     5161 agaagccccg gcggccgagg atccagcggg cggcgcgcag ggcaccgcgg gcgaatccgg
     5221 cacggctgcg ggcgctgtgc tcgagggtga tggtatcagc ctcggagtcg aagccgatcc
     5281 ggtgggtgcc ggggatggcg ccggcgcggt tgctggccac gtcgacgcgg cggtcatagc
     5341 ccgcccgctc catcacccgc tggatctcct tgagcgtccc cgagggggcg tccttcttgt
     5401 ggcgatgatg gatctcgtag agccaggcgt catagccgtc ctcgccggag aggaggcgcg
     5461 ccgccgcctc ggccagccgg tagaagacct ggacgccgac cgagaagttg gcgccatgga
     5521 ggaggccgga gccgtggcgc tccacggcgg cgcgcacccg gggcaggtcg tcgtgccagc
     5581 cggtggtgcc caccaccagg gggattccca gcgcggccag gcgctcggcg ttggccgcca
     5641 ccgcggcggc ggtggagaag tcgatcgcca cctcggcgcc gccaaggtgc tcggcgtcga
     5701 tgctcttccc tgcggcattt tcagagccgg tcagccgcgc caccacctcg aagccgtgct
     5761 ccggcgcgag ctgctcgacg aggcggccca tcttgccgtg gccgaccagg gccaagcggg
     5821 tcatccgatc gctcctgggg gctcgccggc gaacagccgt tcatggagca gagccaccac
     5881 ctcgtccagc ctcgcctcct ccaccaggca gcggaccacc ggacaggcgg ccccctgggt
     5941 gatcaggcgc ggctcgtact cccgggccgc ctccagcacc cgcgccacca gctcgggatg
     6001 ggtgcccagg tcctgcgaga ccagcgacac gacgccggtg ccgggcgaga cctccacctg
     6061 cgacgtccgc ccgaggtcgg agcggatctc gttcaggcgc tcgttccggt cgagcgccag
     6121 ctccacctct ccgtcggcga cgctcagcac ctggagcgcc ggccggaagc gctcgcacgc
     6181 gtcgagcgcc caggagagga agccgttgcg cgcccacggg ccgttggcgg cgacgcgcag
     6241 ccggtgcgcg ttgccccggc aggtgatgga gcggacgcgc ggcggcccct gcggcgcccg
     6301 cgggccgatg atggtcccct cgcccggctc gccatcatgc ggcgaatgca gcgagctgag
     6361 gatgcggatg gggacggcgg cgcggctcgc cgggccgagg gtcccggggt ggggcttctt
     6421 ggccccggag caggccagct ccagcgcctc gccgaaggag aggcagcgca ggcggcgcgc
     6481 gcccggcacg acgcgcgggt cggcggtcag cagcccctcg acgtcggtcc agatctggat
     6541 ctcctcggcc cccagggcgg cgccgacgac ggcggcggtg aagtccgacc cctccttgcc
     6601 cagagtggtg gtgaggccgt cggcggtcga tcccacatag ccgccgagga ccgccacggc
     6661 gccgcgctcg accaccgccg gcacggaacc gcgcaggcgg gcgtcggtgg gaccgtagag
     6721 cggccgggcg cgggtgaact cggcgtcggt gacgatcacc cggcggcagt cgacccattc
     6781 ggcggcgacg ccgtggtggg tgagggccgc
//
LOCUS       DASPJI010000866        16429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_278434, whole genome shotgun sequence.
ACCESSION   DASPJI010000866 DASPJI010000000
VERSION     DASPJI010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 16429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16429)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16429
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278434"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VEL74_24295"
     CDS             complement(<1..544)
                     /locus_tag="VEL74_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYG65727.1"
                     /translation="MTEPLIVTAEGPVRVIRLHRPRVRNAVDLATAHALRRAFLELEA
                     DQEARVAILTGGDEVFCAGADLSDVEALAGEVLGEEGPLGFTRLAVGKPTIAAVAGYC
                     VAGGLELACWCDLRIADETAVFGCFERRFGVPLIDGGTQRLPRIVGLGRALDLILTGR
                     PVAAAEALAMGLVNHVVPRGH"
     gene            complement(556..2355)
                     /locus_tag="VEL74_24300"
     CDS             complement(556..2355)
                     /locus_tag="VEL74_24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HYG65728.1"
                     /translation="MKLPSILVCLLLAAASGLAAQTAAQTSPGAESARLHDLFRREWE
                     TQLRENPVYATIVGRHDYDDRLPAMGRADLERRNGLWKGFLEELKRIDRSKLPAEDVV
                     SAEIFESELEDRIADYELGGWQMPFNADSGFHSDFAFLAAQTTFRSVKDYDNYIRRLR
                     QWPRYMNEQIELMRVGLGRGMTVPRAVLDGYDATISAHLVEDPEQSVFYGPFRDLAQN
                     LPALPAAERQRLEREGRQVVTEAMVPGYRTFLDFYQKEYLPKARTTLGASELPEGRAF
                     YAQQIRHFTTLDLTPEEIHRTGLAEVERIDREMKEVIRQTGFQGDFAAFLQHLRTDRR
                     FYPENGEALLKQAAWIAKRMDGKLPALFGRLPRLPYTVEPVPDHIAPKYTSGRYFEPP
                     QGGTQPGTYWVNVYKPETRPLYNLEALTLHEAVPGHHLQISLARELEGLPEFRRFLYI
                     SAFGEGWGLYSEWLGLEAGFYTDPYSNFGRLTYEMWRACRLVVDTGLHAMGWTRQQAL
                     DYMASHTALPLHEVETETDRYISWPGQALAYKMGELKIKELRRRAEKELGPRFDVRAF
                     HDAVLGSGSIPLGVLEMNVDRWITRVKGEGAGR"
     gene            2521..3834
                     /locus_tag="VEL74_24305"
     CDS             2521..3834
                     /locus_tag="VEL74_24305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65729.1"
                     /translation="METEYDDAEVRHRITEFLGGPSLEEAKCVFLVGYGPAGEAHGPA
                     PPSVLPDWLEQGLDVRRSLWDRGSLLAVLDIGYVDFDRPAEAGLDRQRVSALLEPVAA
                     AAGAILAEHGIVALHLRSGRGHQLVWRIRRGSRAFARLASAGQVPPRLRELYARPHPP
                     SDEPVPREMGAAYAALALVMELVAHRIKRKAAPLCTVPVEITNLAVGPGERGREMISI
                     DLSGYADPLSARTLRVPFTGLDGAPEPAAGTCCLIPDAAEGMERLVDYYRASALSQFH
                     TWFYSQEPAPPPELDETSALLELERLPPCARTILSCPGGLLSASSGVERVTRVLLSFG
                     WHPRHIAGLVRSRYERTGPWGEGWNARDPGSLADFYVRLFSGLFFVGTDDLVSFNCQS
                     ARESGFCFHPVCSCNLLDYRGPLLERRAHGRLARRPLDRVVGGEE"
     gene            complement(3847..4428)
                     /locus_tag="VEL74_24310"
     CDS             complement(3847..4428)
                     /locus_tag="VEL74_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HYG65730.1"
                     /translation="MVVASAEIHRWTREEYERMAAKGFFPPDARVELIEGIVYDTTPQ
                     SSFHSTSVYMLHERLRAVFTEGVIVRNQSPLVLGEDSEPEPDLAVVPGSAEDYLFAHP
                     TTAVLVVEVADSSLLRDRRLKIPLYARAGVPEAWLLNLKRTVLEAYRDPAEGVYRSRT
                     VLRPGESVSPLARPDVSLAVGDFFPWERSARKP"
     gene            complement(4590..4964)
                     /locus_tag="VEL74_24315"
     CDS             complement(4590..4964)
                     /locus_tag="VEL74_24315"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HYG65731.1"
                     /translation="MIQGGTPTIYVSDMERAVTFYTRTLGLKLLEREDDDWASIDAGD
                     GLVLGLQPANGNGDRAAGSGSMSIGFNVTQPLEEVVDKLKHRGVSFRGPIQGKEQAPV
                     RFAFFGDPDGNALYLCELAEME"
     gene            complement(5066..5602)
                     /locus_tag="VEL74_24320"
     CDS             complement(5066..5602)
                     /locus_tag="VEL74_24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HYG65732.1"
                     /translation="MAKSEGLLGKKTARKGTAAPEVRTGPRETGDGDGGGITQEEFLE
                     GLNIDLAHEYAAVIQYRTYASQVQGQWRMELRQFFEQEIPDELGHAQILADKIVALGG
                     TPTGTPAPVKPARDSREMLRNSLEDEIETIERYVKRRKQAEQLGHYGLAVEFDDLIRD
                     ESTHRDEINMILKRWDTG"
     gene            5719..7086
                     /locus_tag="VEL74_24325"
     CDS             5719..7086
                     /locus_tag="VEL74_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HYG65733.1"
                     /translation="MRTIRIGNGQGFWGDNVDAPVELLRGGPIDYIGMDYLAEVTLSI
                     MMRQKLKDPRLGYATDFIGFIRRVLPEALERNIRILSNAGGLNPRACRARILDVAREL
                     GLTGLRVGVVEGDDLLPRLKSLIAKGHALKNMDTGEPVGPLVDKVMNANAYLGAWPVV
                     EALKKGAHIVLCGRITDTALALAPMIHEFGWQREDWDRMAAGTIAGHIIECGAQCTGG
                     NFSRWWEVPDLWNVGYPIIEGREDGTFVVTKQEGTGGMVTIDTVAEQLCYEMGDPGGY
                     ITPDVTADFTSIRLRQDGKDRVEVSGIQGQPSTPFLKVSASYLAGFKASGQITLSGPR
                     AVDKARLAAEVVWKRLERAGVTFDEEHRSEEILGTGACLPGIFQTPDELPEVVLRLGV
                     RDPDPIKVERFGKEIAPLVTAGPPGVTGFAGGRPKPQEIVAYWPALLPREEVESRIEV
                     TVEEV"
     gene            7083..7529
                     /locus_tag="VEL74_24330"
     CDS             7083..7529
                     /locus_tag="VEL74_24330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65734.1"
                     /translation="MSAASPEIQPAERELLVRWYAGLLGGLLPILLLGFLYSALADRL
                     EFVGWALGLGVVWVIALRHGLGAGWPRPRLWGTLALLLALGLGGFSAIERRHHEILDL
                     GFRAVFPNAYHPVATRPGTAAALAGLLALTGGAALAIHGIRQRRAR"
     gene            7526..7864
                     /locus_tag="VEL74_24335"
     CDS             7526..7864
                     /locus_tag="VEL74_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65735.1"
                     /translation="MKVKLDRVAHARSGDKGDASNVGLIAANERLYEVIREQATAERV
                     KEHFRDVCRGPVDRYELPNILALNFILHDSLGGGGTESLKNDAQGKTHAQGLLQMEVE
                     VPDELLEGRP"
     gene            7861..8694
                     /locus_tag="VEL74_24340"
     CDS             7861..8694
                     /locus_tag="VEL74_24340"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HYG65736.1"
                     /translation="MSGLADLRFERLGVRWDGDLIWVTLSDPERANALSPAMIVELTE
                     LYRRNLREEGIRAVLLAGAGKNFSAGADLEHLRSLRDAGEEENLRDSMRLKNLFDAVL
                     RQDALTIALVHGACVAGGCGLATAHDFVVAAEDARFLYSEVRIGFVAALVATYLPLRL
                     RGSDIRELLLFPRFIDAPRALEIGLANRVVPAADLTAVGEALAAEILETGSSESIART
                     KKLLLDVLGKPLPEALDHAARVNAASRATADCKHGIATFLETKKPPVWREKRPGTSEP
                     S"
     gene            8691..9392
                     /locus_tag="VEL74_24345"
     CDS             8691..9392
                     /locus_tag="VEL74_24345"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HYG65737.1"
                     /translation="MSRLILFDIDGTLVDCGPQVRPLFASALEEVFGETGDIHGYSFA
                     GRTDPRIVLDLLTGAGMSEKEVRSRLPWMREVYLAKLERALAREGMRLLPGVQEMLES
                     LEARDDVVLALLTGNWEPGARTKLSRFDLNRYFAFGAFGCDAADRDHLPPVALDRAEQ
                     WTGRRFTPEETLIIGDSIHDVSCGRAHGIPVLAVATGHTPAQALHDAGADWVIPDLRV
                     AAESVEWLEAVAGRG"
     gene            complement(9396..10238)
                     /locus_tag="VEL74_24350"
     CDS             complement(9396..10238)
                     /locus_tag="VEL74_24350"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HYG65738.1"
                     /translation="MTTTPFTLDTPSGATLHGFADLPDGPPEPRPAVVICHGFKGFME
                     WGFFPYLGTLLAERGFVAVRFNVSGSGMRQGEDLVGDPEGFRANTHGREVADFLAVLE
                     AAGEAIAPGWVDRSRIGIFGHSRGGGNAVLLASRSPWRERLRAVVTWAAVSSFDRYTL
                     EQKEVWRRDGEIPVVNARTGQQLAMGVALLDEIETRGQELNILAAAAEVRAPWLIVHG
                     EEDESVAADEARRLDAAAQGPHELLLVPRGNHTFGARHPFAGPTRELIQAMNATQRWF
                     RRHL"
     gene            complement(10250..10411)
                     /locus_tag="VEL74_24355"
     CDS             complement(10250..10411)
                     /locus_tag="VEL74_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65739.1"
                     /translation="MKTEKKFDAVKAMREIREEISREIQGMSFEQERDYIENSLKPGR
                     EREKVGAQS"
     gene            complement(10401..10725)
                     /locus_tag="VEL74_24360"
                     /pseudo
     CDS             complement(10401..10725)
                     /locus_tag="VEL74_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain protein"
     gene            11031..11588
                     /locus_tag="VEL74_24365"
     CDS             11031..11588
                     /locus_tag="VEL74_24365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65740.1"
                     /translation="MRSPIEGNTPRVPNSVPEQQSSNAFSKSFLQADLDLNEPAAIDD
                     PEGEGPWLVLPASPDGWPEPWEEVHGMWPLGERPERGDPPAFLSSGRSVALCASIARP
                     VAGRDPLYGLGQEKWRGGFPLLRQGRPEAWMGSFFPEWALITSALLSLGQRPEAVAVY
                     LEALGPSCRRRIGAILYERLLGARR"
     gene            11944..13026
                     /locus_tag="VEL74_24370"
     CDS             11944..13026
                     /locus_tag="VEL74_24370"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HYG65741.1"
                     /translation="MASTPTTSAPSLALASLHERLGATLTPLDGNGSLVPARYGSTEE
                     EYRALREGCGVADLSWRGLLEVTGKDRQRFLNAYVTCDVKAIAPGQGAYGFFTNPQGR
                     ILSDLVLAALEDRFWMLVGPGQEEGLAGHLQKYILADRVEIRPLEGVVPLALIGPRAG
                     EALGIAELPAPWSHVRAAVEGVEVTVQGSERFGQPAWILWAPAAEAGALLEGLAAGGA
                     RPVGFEAQETLRVEAGIARFGRDFGPDNFPQETGAEEAAVSYTKGCYLGQEVVARIHY
                     RGGVQKGLRGVVFETDSGVASGTEVFHDGRPAGTVTTVVHSFALGRPAGLAILHKRAA
                     EPGTRLQMDSIESVGTVEVRELPLAP"
     gene            complement(13023..13307)
                     /locus_tag="VEL74_24375"
     CDS             complement(13023..13307)
                     /locus_tag="VEL74_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002750041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HYG65742.1"
                     /translation="MPRALWNGQVIAETDKHELVEGNVYFPPEAIRREFFQPSTTTTV
                     CGWKGTANYYNVVVNGQENKDAAWYYADPKPAAANIKDHIAFWRGVTVER"
     gene            13380..14654
                     /gene="egtB"
                     /locus_tag="VEL74_24380"
     CDS             13380..14654
                     /gene="egtB"
                     /locus_tag="VEL74_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HYG65743.1"
                     /translation="MDREQILSRYRETRALSVELCRPLRPEDYRVQSMPDVSPPWWNL
                     GHTTWFFAKNVLEPFGLYRDEDARLEYVLNSYYESLGPRIARDHRGLVTRPTTEEVYR
                     FRESVDERMEELIHGAGQGDLERLDFLVHTGIQHEQQHQELLLTEIKHILGSNVPDLR
                     EPYRPAASGLRRGTSSPAWVELEAGLHEFGNVEGGWAWDNEMPVHKGWLDGFALMSRL
                     VTNGELLEFVEAGGYRDPLLWLSNGWARVRDLGWTAPLYWEQGDGGSWQVWTLSGTQG
                     LDLDEPACHVSFYEADAYARWKGARLPTEREWEHAARLSGFPGPGANFLDSGAFHPVP
                     AGPDGDGSLAQMGGDLWEWTTSHYEPYPGYRPFDGALMEYNGKFMDNQRVLRGGSCAT
                     PASHIRASYRNFWPADARFQFTGIRLAKDLRG"
     gene            14656..15105
                     /locus_tag="VEL74_24385"
     CDS             14656..15105
                     /locus_tag="VEL74_24385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006272201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HYG65744.1"
                     /translation="MSRRWLLRLMNVYPPFLGAGVRVRASADLRTFDVRMGLHRWNRN
                     YVGTHFGGSLYTMCDPFFMLILVEGLGRGYIVWDKQASIRFRRPGRGTVRATFHIPAG
                     RIEEIRADADRLGKVEPTFHVDVLDEEGEVVAEVEKLLYVRKKGEVR"
     gene            15156..15890
                     /locus_tag="VEL74_24390"
     CDS             15156..15890
                     /locus_tag="VEL74_24390"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HYG65745.1"
                     /translation="MDRRALTCLALLLTLSIPAAADTLLTVKSHVGAFQLMGENQPAK
                     DTEVKIWVGADRLRRDEGQTSSILRLDRNKLYIVDHAAKTYSEVGLPVDLQKYAPAGT
                     EAAMKQMGDAMRLDAKVTPASETRKIGDWNARRVDVAITSAMGMKIATTMWVSKDLEI
                     YRSLNRMSATLASLQHGAAAWARQLEEIEGFPVLQESSISVVGTQFKTREELVAVAAQ
                     DAPAGIYEPPAGYTRQPFNPMAQPGR"
     gene            complement(15931..>16429)
                     /locus_tag="VEL74_24395"
     CDS             complement(15931..>16429)
                     /locus_tag="VEL74_24395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65746.1"
                     /translation="RRVFGDLRSGLISATVALNLMHIVSESVERAVLFLVKRDHLMAL
                     GAFGVDTRGKPLAEVTRALKLGLESDSALTRAMETGEVQALSFDDANLPEPLRSMVGS
                     PRTGQVVVFPVLGAQRVISVIYTDNGDRDQPIEDIDILELATAQVGMAFENELLRRQI
                     SQHRE"
BASE COUNT         2473 a         5799 c         5600 g         2557 t
ORIGIN      
        1 cgtggcctcg cggcaccacg tggttgacca gccccatggc gagcgcctcc gcggcggcga
       61 ccggccggcc ggtgaggatc aggtcgaggg cgcggccgag accgacgatg cgggggaggc
      121 gctgggtgcc gccgtcgatc agggggactc cgaagcggcg ctcgaagcag ccgaagacgg
      181 cggtctcgtc ggcgatccgc aggtcgcacc agcaggccag ctccaggccg ccggcgacgc
      241 agtaccccgc caccgccgcg atcgtcggtt tccccaccgc cagccgggtg aaccccagcg
      301 gcccctcctc ccccagcacc tcgccagcca gcgcctcgac atccgagagg tccgcgccgg
      361 cgcagaacac ctcgtccccg ccggtgagga tggcgacccg cgcctcctgg tccgcttcga
      421 gctccaggaa cgcccgccgc aacgcgtggg cggtggcgag gtccaccgcg ttccgtaccc
      481 gcggccggtg gagccggatg acgcggaccg gtccctcggc ggtgacgatc aggggctcgg
      541 tcatgagcag cctccctacc tccccgcccc ctcccccttc acccgcgtga tccagcggtc
      601 gacgttcatc tccagcaccc ccagagggat ggagccgctg cccaggacgg cgtcgtggaa
      661 ggcgcggacg tcgaagcggg ggcccagctc cttctcggcg cggcggcgca gctccttgat
      721 cttgagctca cccatcttgt aggccagagc ctggccgggc catgagatgt agcggtcggt
      781 ctcggtctcc acctcgtgca gcgggagcgc ggtgtgcgag gccatgtagt cgagggcctg
      841 ctgccgcgtc cagcccatgg cgtggagccc ggtgtccacc accagacggc aggcgcgcca
      901 catctcgtag gtcaggcgcc cgaagttgct gtagggatcg gtgtagaagc cggcctccag
      961 gcccagccac tccgagtaga gcccccaccc ttccccgaag gccgagatgt agaggaagcg
     1021 ccggaactcc ggcaagccct cgagctcgcg ggcgagggag atctgcaggt gatggcccgg
     1081 caccgcctcg tgcagcgtca aggcctccag gttgtagagc ggccgggtct ccggcttgta
     1141 gacgttgacc caataggtgc ccggctgggt ccccccctgg ggcggctcga agtagcgccc
     1201 cgaggtgtac ttgggcgcga tgtggtcggg caccggctcc accgtgtagg ggaggcgtgg
     1261 cagccggccg aacagcgccg ggagcttgcc gtccatgcgc ttggcgatcc acgccgcctg
     1321 cttgaggagg gcctcgccat tctccgggta gaaccgccgg tccgtccgca gatgctgcag
     1381 gaaggcggcg aagtccccct ggaatccggt ttggcggatc acctccttca tctccctgtc
     1441 gatccgctcc acctcggcga ggccggtgcg gtggatctcc tccggcgtca ggtcgagcgt
     1501 ggtgaagtga cgtatctgct gggcgtagaa ggcgcgccct tcgggcagct ccgaggcgcc
     1561 gagggtggtc cgcgccttgg gcaggtactc cttctgatag aagtcgagga aggtccggta
     1621 tcccggcacc atcgcctccg tcaccacctg ccgcccctcc ctctccagcc gctgccgctc
     1681 ggcagccggg agcgccggaa ggttctgggc gagatcccgg aacggaccgt agaagacgct
     1741 ctgctccggg tcctccacca ggtgggcgga gatggtggcg tcgtaaccgt cgaggaccgc
     1801 ccgggggacc gtcatccccc gtccaaggcc gacgcgcatg agctcgatct gctcgttcat
     1861 gtaccgtggc cactggcgca gccgccggat gtagttgtcg tagtccttga cggaccggaa
     1921 ggtggtctgc gccgccagga acgcgaagtc gctgtggaac ccggagtcgg cgttgaacgg
     1981 catctgccag ccccccagct cgtagtcggc gatccggtcc tccagctcgg actcgaagat
     2041 ctcggcgctc accacgtcct cggccggcag cttcgaccgg tcgatccgct tgagctcctc
     2101 caggaacccc ttccagaggc cgttccggcg ctcgaggtcc gcgcgcccca ttgccggcag
     2161 gcggtcgtcg tagtcatgac ggccgacgat ggtggcgtag accggattct cccggagttg
     2221 ggtctcccac tcgcggcgga acaggtcgtg gaggcgcgcc gactcggccc ctggagaggt
     2281 ctgagcggcg gtctgggcgg ccagcccgga ggccgcggcg agcagcaggc agacgaggat
     2341 ggagggcaat ttcatggacg gggctcccaa aaagacagag atataggaaa atgcctctga
     2401 gtttatcctg gagtcaaggc ctccccgtca cccgggggag ccatgaggtt ctatcacgga
     2461 cgcgaggagc ggtccgggcc gcggtgtccg cggaccgggt ctgatggcgt ggagagcggc
     2521 atggagaccg agtacgacga cgccgaggtc cgccaccgca tcaccgagtt cctcggcggt
     2581 ccctcgctgg aagaggcgaa gtgcgtgttc ctggtggggt acggccccgc cggggaggcg
     2641 cacggcccgg cgccgccgtc ggtcctgccg gactggctgg agcaggggct ggacgtccgc
     2701 cgctcgctct gggaccgcgg ctcgcttctc gccgtcctgg acatcgggta cgtcgacttc
     2761 gaccgtcccg ccgaggccgg cctcgaccgg cagcgcgtct ccgccctcct ggagccggtg
     2821 gcggcggccg ccggtgcgat cctggccgag cacgggatcg tggcactcca cctgcggagc
     2881 ggacggggcc atcagctcgt ctggcggatc cgccggggct cccgggcctt cgcgcgcctg
     2941 gcgtccgccg ggcaggtgcc gccgcggctg cgggagctct atgcccggcc gcatccgccg
     3001 tcggacgagc cggtgccgcg ggagatgggg gccgcctatg ccgccctggc cctggtgatg
     3061 gagctggtgg cccaccgcat caagaggaag gcggcgccgc tctgcaccgt gccggtggag
     3121 atcacgaatc tggcggtggg accgggggag cgtggccggg agatgatctc catcgatctg
     3181 tcagggtacg ccgatccgct gagcgcccgg acgttgcggg tgcccttcac cggcctcgac
     3241 ggtgccccgg agccggcggc gggaacctgc tgcctgatcc cggacgccgc ggagggcatg
     3301 gagcggctgg tggactacta ccgcgcctcg gcgctgtcgc agttccacac ctggttctac
     3361 tcccaggagc cggcgccgcc ccctgagctg gatgagacga gcgccctgct ggagctggag
     3421 cgcctcccgc cgtgcgcccg caccatcctc tcctgcccgg gggggctgct gagcgcctcg
     3481 agcggtgtcg agcgcgtgac ccgggtgctc ctctccttcg gctggcaccc ccggcacatc
     3541 gccggcctgg tccgctcgcg ctacgaaagg accggcccct ggggggaggg ctggaacgcc
     3601 cgcgacccgg gcagcctcgc cgacttctac gtccggctct tctccggcct cttcttcgtg
     3661 ggaaccgacg acttggtgag cttcaactgc caatccgccc gtgaatcggg cttctgcttc
     3721 cacccggtat gctcctgcaa cctcctggac taccggggcc cgctgctgga gaggagggct
     3781 cacgggcgcc tcgcccgcag gccactggac cgggtggtgg gaggggagga atagggccga
     3841 ggcgcactac ggcttccgcg ccgagcgctc ccagggaaag aagtccccca ccgcgagcga
     3901 gacgtccggg cgtgccaggg gagagaccga ctcccccggg cggaggacgg tccgcgaacg
     3961 gtagacgcct tcggcgggat cgcgataggc ctccagcacc gtccgcttca ggttgagcag
     4021 ccaggcctcg gggacgccgg cccgggcata gagggggatc ttcagccgcc ggtcgcggag
     4081 gagcgacgag tcggctactt ccacgaccag caccgccgtc gtcggatggg cgaacaggta
     4141 atcctcggcg ctgccaggca ccaccgccag atcgggctcg ggctcggagt cctccccgag
     4201 aacgagcggc gactggttcc gaacgatgac gccctcggtg aagaccgccc gcaggcgctc
     4261 atgcagcatg tacacactcg tactatggaa gctgctctgc ggcgtcgtgt cgtagacgat
     4321 gccctcgatg agctcgaccc tggcgtccgg cgggaagaac cccttggcgg ccatgcgctc
     4381 gtactcttcc cgcgtccagc gatggatctc ggcggatgcg acgaccatga gacgaatacc
     4441 tcctgaagac tatcacaagc ccgggaaacg cgaccggacc cgcgtgctcg ccgatcttac
     4501 cgcacgagcc aagggctgga gcgcgtcatc tccccggtca tccggaaaga caccgcaccc
     4561 gccccgccgg caaccggcgc cgcccgcctc tactccatct cggcgagctc gcagaggtac
     4621 agggcgttgc cgtcaggatc accgaagaag gcgaagcgga caggcgcctg ttccttgccc
     4681 tggatggggc cgcggaagct gacgccgcga tgcttcagct tgtcgacgac ctcctccagc
     4741 ggttgggtga cgttgaagcc gatcgacatg gagccggacc cggccgcccg gtctccgttg
     4801 ccgttggcgg gctggagccc gaggaccagg ccgtccccgg catcgatgga cgcccagtcg
     4861 tcgtcctccc gttcgaggag cttcagaccg agcgtccggg tatagaaggt gaccgcgcgt
     4921 tccatgtcgc tgacgtagat cgtcggcgtc ccaccctgga tcatggacat ctccttgagc
     4981 tggcccatac accaggggac cggacgtcct tggccggtcc ccctttacaa aacaagtgct
     5041 agttctccaa cgaagcgctc ccggcctagc cggtgtccca ccgcttgagg atcatgttga
     5101 tctcgtcgcg gtgcgtgctc tcgtcgcgga tcagatcatc gaactcgacc gcgaggccgt
     5161 agtggccgag ctgctcggcc tgcttccgcc gtttgacgta gcgctcgatc gtctcgatct
     5221 cgtcctccag ggagttgcgg agcatctcgc gggaatccct ggccggtttg accggcgccg
     5281 gcgtcccggt gggcgtgccg cccagggcga cgatcttgtc cgccaggatc tgggcgtggc
     5341 ccagctcgtc cggaatctcc tgctcgaaga actgccgcaa ctccatgcgc cactggccct
     5401 gcacctggct ggcataggtc cggtactgga tcaccgccgc gtactcgtgg gccagatcga
     5461 tgttgagtcc ttcgaggaac tcctcctggg tgatcccgcc gccgtcgccg tcccccgtct
     5521 cgcgaggtcc ggtcctcacc tcgggcgccg ccgtcccctt cctcgccgtc ttcttaccca
     5581 agagaccttc actcttcgcc atacgaacgt gcctcctttc actcgggatc ttgatatgca
     5641 aaatccgccc ccaccgactt ccgggacgcc ccgggcctcc cggcgccagc ttacacccgt
     5701 gctaggctag ggcgctctat gagaacgatc cgtatcggca acggccaggg cttctgggga
     5761 gacaacgtgg acgcgccggt ggagctgctg cgcggcggtc ccatcgacta catcggcatg
     5821 gactacctcg ccgaggtgac cctgtcgatc atgatgcgcc agaagctcaa ggacccgcgg
     5881 ctgggctatg ccaccgactt catcggcttc atccgccgcg tgctccccga ggcgctggag
     5941 aggaacatcc gcatcctctc gaacgccggc ggcctcaacc cgcgggcctg ccgcgccagg
     6001 atcctcgacg tcgcccgcga gctcggcctc accggcctgc gcgtcggcgt cgtcgaagga
     6061 gacgacctcc tgccgcggct caagagcctc atcgccaaag gccacgcgtt gaaaaacatg
     6121 gacaccggcg agcccgtcgg acccctggtc gacaaagtga tgaacgccaa cgcctacctg
     6181 ggggcctggc cggtggtcga ggccctgaag aaaggcgcgc acatcgtcct ctgcggccgg
     6241 atcaccgaca cggccctggc gctggcgccg atgatccacg agttcgggtg gcagcgcgag
     6301 gactgggacc gcatggccgc cggcaccatc gccggccaca tcatcgagtg cggcgcccag
     6361 tgcaccggcg gcaacttctc gcgctggtgg gaggtgccgg acctctggaa cgtcggctat
     6421 ccgattatcg aggggcgcga ggacggtacc ttcgtcgtca ccaagcagga agggaccggc
     6481 ggcatggtca ccatcgacac cgtggccgag cagctctgct acgagatggg cgacccgggc
     6541 ggctacatca cgccggacgt gaccgccgac ttcacctcca tccgcctgcg ccaggacggc
     6601 aaggaccgcg tggaggtctc cggcatccag ggacaaccca gcacgccgtt cctcaaggtc
     6661 tcggcctcct acctcgccgg cttcaaggcg agcggccaga tcaccctctc cggcccacgg
     6721 gctgtcgaca aggcgcggct cgccgccgag gtcgtctgga agcggctgga gcgcgccggc
     6781 gtcaccttcg acgaggagca ccggtcggag gagatcctcg gcaccggcgc ctgcctgccc
     6841 ggcatcttcc agacgccgga cgagctgccc gaggtggtgc tgcggctggg cgtgcgcgat
     6901 cccgacccca tcaaggtgga gcgcttcggc aaggagatcg cgccgctggt caccgccggc
     6961 ccgccggggg tcaccggctt cgccggcggc cgccccaagc cgcaggagat cgtggcctac
     7021 tggccggccc tccttccccg cgaggaggtg gagagccgca tcgaggtcac cgtggaggag
     7081 gtgtgagcgc cgcctcaccg gagatccagc cggccgagcg cgagctcctg gtccgctggt
     7141 acgccggcct cttgggcggg ctcctgccga tcctcctcct cggctttctc tacagcgcgc
     7201 tggccgaccg gctcgagttc gtcggctggg ccctgggtct cggcgtggtc tgggtgatcg
     7261 ccctgcgcca cggcctgggc gccggctggc cgcgcccccg tctctggggg acgctggcgc
     7321 tgctcctggc gctggggctc ggcggcttct cggccatcga gcgccggcat cacgagatcc
     7381 tggacctggg cttccgcgcc gtcttcccga acgcctatca tcccgtcgcc acccgccccg
     7441 gcacggccgc ggccctcgcg ggcctcctgg ccttgaccgg aggcgcggcg ctggcgatcc
     7501 acggaatccg tcagaggaga gcacgatgaa ggtgaagctg gaccgggtgg cccatgcccg
     7561 ctcgggcgac aagggggacg cgagcaacgt cggcctgatc gctgccaacg agcgcctcta
     7621 cgaggtgatc cgcgagcagg cgacggccga gcgggtcaag gagcacttcc gggacgtctg
     7681 ccgcggcccg gtggaccgct acgagctgcc gaacatcctg gcgctgaact tcatcctcca
     7741 cgattcgctg ggcggcggcg gcaccgagag cctcaagaac gacgcccaag ggaagaccca
     7801 cgcccagggg ctcctgcaga tggaggtcga ggttccggac gagctattgg aggggcggcc
     7861 gtgagcggcc tcgcggacct gcgcttcgag cggctcggcg tgcgctggga cggcgacctg
     7921 atctgggtga cgctcagcga tccggagcgg gccaacgccc tgtcgccggc gatgatcgtc
     7981 gagctgaccg agctctatcg ccgcaatctg cgggaggagg ggatccgggc ggtgctgctg
     8041 gcgggtgccg gcaagaactt ctcggccggg gcggacctcg agcacctgcg ctcgctgcgc
     8101 gacgcgggcg aggaggagaa cctccgcgat tcgatgcgcc tcaagaacct gttcgacgcg
     8161 gtgctgcgcc aggacgcctt gaccatcgcc ctggtgcacg gggcctgcgt cgccggcggc
     8221 tgcggcctcg ccacggcgca cgacttcgtg gtcgccgccg aggacgcccg cttcctctac
     8281 agcgaggtgc ggatcggctt cgtggcggcg ctggtcgcca cctacctgcc cctgcgcctg
     8341 cgcggctcgg acatccgcga gctgctcctc ttcccgcgct tcatcgacgc cccccgggcg
     8401 ctggagatcg gcctcgccaa ccgcgtcgtc cccgccgccg acctgacggc ggtgggcgag
     8461 gcgctggcgg cggagatcct ggagacgggc agctccgagt cgatcgcccg caccaagaag
     8521 ctgctgctgg acgtcctcgg caagccgctg ccggaagcgc tcgaccacgc cgcgcgggtc
     8581 aacgccgcct cccgggccac ggccgactgc aagcacggca tcgccacctt cctcgagacc
     8641 aagaagccgc ccgtctggag agagaagcga ccggggacca gcgagccgtc atgagccgcc
     8701 tgatcctctt cgacatcgac ggcaccctgg tcgactgcgg cccgcaggtc cggccgctct
     8761 tcgcctccgc gctggaggag gtcttcggag agaccgggga catccacggc tacagcttcg
     8821 ccggccggac cgatcctcgc atcgtcctgg accttctgac aggtgccggg atgtccgaga
     8881 aggaagtgcg ctcccggctc ccctggatgc gcgaggtcta tctcgcgaag ctggaaaggg
     8941 ccctggcgcg ggaggggatg cgcctgctcc ccggggtgca ggagatgctg gagagcctgg
     9001 aggcgcggga cgacgtggtc ctggcgctcc tgacgggcaa ttgggagccc ggcgcccgca
     9061 ccaagctgtc gcgcttcgac ctcaaccgct acttcgcctt cggagccttc ggctgcgacg
     9121 ccgccgaccg cgaccacctc cccccggtgg ccctcgaccg cgccgagcaa tggaccggcc
     9181 gccgcttcac ccccgaagag acgctgatca tcggtgacag catccacgac gtctcctgcg
     9241 gccgcgccca cggcatcccc gttctcgccg tcgccaccgg ccacaccccc gcccaggccc
     9301 tccatgacgc gggagccgat tgggtcatcc cggatctgcg ggtggcggcg gagtcggtgg
     9361 agtggttgga ggcggtggcg gggagggggt agggatcaca ggtgcctccg gaaccaccgc
     9421 tgagtagcat tcatcgcctg gatgagctcc cgcgtggggc cggcgaacgg atgccgagcc
     9481 ccaaaagtat ggttccccct aggaacgagt aacagctcat gcggcccttg tgccgccgcg
     9541 tcgagccggc gcgcctcgtc ggcggccacg ctctcgtcct cctctccatg aacgatgagc
     9601 cacggcgccc ggacctcggc cgccgcggcg aggatgttca gttcctgccc ccgcgtctcg
     9661 atctcgtcca gcagggccac ccccatggcg agttgctggc cggtgcgggc gttgactacg
     9721 gggatctcgc cgtcgcgacg ccagacttcc ttctgctcca gggtgtagcg gtcgaagctg
     9781 gagaccgccg cccaggtgac cacggcccgt agccgctccc gccaggggga ccgcgatgcc
     9841 aggaggacgg cgttgccccc gccgcggctg tgcccgaaga tcccgatccg cgagcggtcc
     9901 acccatccgg gggcaatggc ctcccccgcc gcctccagca ccgccaggaa atccgccacc
     9961 tcccggccgt gggtgttcgc ccggaacccc tcgggatcgc ccaccaggtc ctccccctgc
    10021 cgcatccccg atcccgacac gttgaaccgg acggccacga agccgcgctc cgccagcagg
    10081 gtcccgaggt aggggaagaa cccccactcc atgaacccct tgaacccatg gcagatcacc
    10141 accgccggcc gcggctcggg tggtccgtcc gggagatcgg cgaagccgtg gagggtggcg
    10201 ccggagggag tgtcgagggt gaagggggtg gtggtcatgg ggaggcgcct cagctctgcg
    10261 cgccgacctt ctcccgttct cgtcccggct tcaggctatt ctcgatgtag tctctctctt
    10321 gctcgaaact catcccctga atctctcgac tgatctcctc ccgaatctct cgcatcgcct
    10381 tgacggcgtc aaacttcttc tcagtcttca tcaggcgcta cctctcgggg tgtccggatc
    10441 tccagcaggg ggtatccgag acgcagattg acagagttgt acccatggat tctgttgatg
    10501 ttcacgatgt gcttgaaatt ccagctcacc aggacgtcga cacgattgat cgttgctgtg
    10561 gcgatgtgct gcgcatcgag gaggcttcct gcccctatga ccttggcggc agggtacttc
    10621 acggccggtc gcgaaacgtt cgaacagccg cgaagaaaaa agcgagaact cttcgtcaag
    10681 gcagccgccg ataactgagg tatcagtgta gattcttcgc ttcatcgccg tccccgtcgc
    10741 cagccacctc gtcattctcg accctcttcg aacaccgcgc aaccctgcgg accccttccg
    10801 ccctcttgac acccctccca cctcgcacta cagtacatcc gtcgtaacca cagcatagca
    10861 gcctgtcagg agaggaggtg gtagcggagg caggagagac gatagcatcc cccgcgggaa
    10921 gttgaagcga ggcggccggc gcgtggccga ccgccaagac aagacctggt agcggcccgg
    10981 agtgagttgg tgcctcaccc cgagcctgac cacgaaagga gttggtctcc atgcgcagtc
    11041 ccattgaggg taacacccct cgtgtaccca acagcgtgcc cgagcagcag tcctccaacg
    11101 cgttttccaa atccttcctc caggccgacc tcgacctgaa cgagcccgcc gcgatcgacg
    11161 atccggaggg cgagggcccc tggctcgtgc ttccggcgtc tcctgacggt tggcccgagc
    11221 cgtgggagga ggtccacggg atgtggcctc tgggcgagcg gccggagcgg ggcgatccgc
    11281 cggcgttcct gtcgtccggg cggtctgtcg ccctctgcgc ctccattgcc cggccggtcg
    11341 ccgggcgcga tcccctctat gggctcggac aggagaagtg gcgcggcggc ttcccgctgc
    11401 tccggcaggg gcggcccgag gcgtggatgg gcagcttctt ccccgagtgg gcgctgatca
    11461 ccagcgctct cctcagcctc ggacagcggc ccgaggccgt cgccgtctac ctcgaggccc
    11521 tcggcccctc ctgccggcgg cggatcgggg ccatcctcta cgagcgtctc ctcggcgccc
    11581 gccgctgaac cgtccttaat cctgatcggg aggcggagga gcattcctcc gccttcctcc
    11641 tcttcggcca ctcctcgtgg ggcagccact gccccaatag aaatgaacgt cattccttgt
    11701 ggggcaatgg ctgccccggc aggaatcgtc accattcctc ctggggcagc ggctgcccca
    11761 ggaggaacga tggtcattcc tgatggggca gcgattgccc cacaaggaat ggtcgccggt
    11821 tctcgtgggg caacgattgc cccgacagga atggtggtca ttcctcgtgg ggcagcggct
    11881 gccccttcag ggatggtcgt cggggaaggg aggatggaga taggggcctg ctagactccc
    11941 cctatggcca gcacccccac gacctccgcc ccctccctcg ccctcgcctc gctccatgag
    12001 cgcctggggg cgacgctcac gcctctagac ggcaacggta gcctggtgcc ggcgcgctat
    12061 ggctccactg aggaagagta ccgggcgctc cgcgaggggt gcggcgtggc cgacctctcc
    12121 tggcgcgggc tcctcgaggt cacgggcaag gaccgccagc ggttcctcaa cgcctacgtc
    12181 acgtgtgacg tcaaggccat cgctcccgga cagggggcct acggcttctt caccaatccc
    12241 cagggccgca tcctctccga cctcgtgctc gccgcgctgg aggaccgctt ctggatgctg
    12301 gtgggcccgg gacaagaaga aggcctcgcg ggccatctcc agaagtacat cctggccgac
    12361 cgggtggaga tccgtccgct cgaaggggtc gtgcccctgg cgctgatcgg cccccgtgcc
    12421 ggtgaggcgc tggggatcgc cgagctcccc gctccctgga gccatgtccg ggcggcggtt
    12481 gaaggggtgg aagtcacggt gcaggggagc gagcggttcg ggcagccggc gtggatcctc
    12541 tgggccccgg cggcagaggc cggcgcgctg ctggaggggt tggcggcggg cggggcgcgg
    12601 ccggtggggt tcgaggcgca ggagacgctg cgggtcgagg ccgggatcgc ccgcttcggc
    12661 cgcgacttcg ggccggacaa cttcccgcag gagaccggcg ccgaggaggc ggcggtcagc
    12721 tataccaagg gctgctacct gggccaggag gtggtggccc gcatccacta ccggggcggc
    12781 gtgcagaagg gactgcgcgg ggtggtcttc gagacggact cgggcgtggc gtccggcacc
    12841 gaggtcttcc acgacgggcg gccggcgggg accgtcacca ccgtcgtcca ctccttcgcc
    12901 ctcggccggc cggcggggct cgcgatcctc cacaagcgcg ccgccgagcc gggcacccgg
    12961 ctccagatgg actccatcga gagcgtcggg accgtcgagg tgcgggagct gccgctcgct
    13021 ccctagcgct cgaccgtgac gccgcgccag aaggcgatgt ggtccttgat gttggccgcg
    13081 gcgggcttgg ggtcggcgta gtaccaggcg gcgtccttgt tctcctggcc gttcaccacc
    13141 acgttgtagt agttggcggt ccctttccag ccgcagacgg tggtcgtcgt gctgggctgg
    13201 aagaactcgc ggcggatggc ctcgggtggg aagtagacgt tcccctccac cagctcgtgc
    13261 ttgtccgtct cggcgatcac ctgaccgttc cacagcgctc tcggcatctc tctcctcctc
    13321 tctctctccc ggacgggaac cgtcccgcat gcacggcgat tgtatatagg atgtccccca
    13381 tggaccgcga gcagatcctg agccgctacc gcgagacccg cgccctgagc gtggagctgt
    13441 gccgtcccct gcgccccgag gactaccggg tccagtccat gccggacgtg agccccccct
    13501 ggtggaacct ggggcacacc acctggtttt tcgccaagaa cgtcctcgag cccttcggcc
    13561 tctatcgcga cgaggacgcg cggctggagt acgtcctcaa ctcctactac gagtcgctgg
    13621 ggccgcgcat cgcccgcgat caccgggggc tggtgacccg ccccaccacg gaggaggtct
    13681 atcgcttccg cgaatcggtg gacgaacgga tggaggagct gatccacggc gccgggcagg
    13741 gggatctcga acggctcgac ttcctggtgc acaccggcat ccagcacgag cagcagcacc
    13801 aggagctgct gctgaccgag atcaaacaca tcctggggtc gaatgtcccc gacctgcggg
    13861 agccctaccg gcccgccgcg tccggactgc ggcgcggcac ctcctccccc gcctgggtcg
    13921 agctcgaggc ggggctccac gagttcggca acgtcgaggg gggctgggcc tgggacaacg
    13981 agatgcccgt ccacaagggg tggctcgacg gcttcgcgct gatgagccgc ctggtgacca
    14041 acggcgagct cctggagttc gtcgaggccg gcggctaccg cgatcccctg ctctggctct
    14101 cgaacggctg ggcccgggta cgggacctcg gctggaccgc ccccctctat tgggagcaag
    14161 gggacggagg gagctggcag gtctggaccc tctcggggac gcaaggtctg gatctggacg
    14221 agccggcctg ccacgtcagc ttctacgagg ccgacgccta cgcccgctgg aagggggcgc
    14281 gcctgcccac cgagcgcgag tgggagcacg ccgcccgcct ctccggcttc cccggcccgg
    14341 gcgccaactt cctggacagc ggcgccttcc accccgtgcc cgcgggcccg gacggcgacg
    14401 ggtcgctcgc gcagatgggg ggcgacctgt gggagtggac cacgagtcac tacgagccct
    14461 acccgggcta ccggcccttc gacggcgcgc tgatggagta caacggcaag ttcatggaca
    14521 accagcgggt gctgcgcggc ggctcctgcg ccacgccggc gtcgcacatc cgggccagct
    14581 accgcaattt ctggccggcc gatgcccgct tccagttcac cggcatccgg ctggcgaagg
    14641 acctgcgggg ctgagatgtc gcgccgctgg ctgctccgcc tgatgaacgt ctatccgcct
    14701 ttcctcggcg cgggggtgcg cgtccgcgcc tccgccgacc tgcgcacctt cgacgtgcgg
    14761 atgggcctgc accggtggaa ccgcaactac gtgggcaccc acttcggcgg ctcgctctac
    14821 accatgtgcg accccttctt catgctgatc ctggtagagg ggctggggcg cggctacatc
    14881 gtctgggaca agcaggcctc catccgcttc cggcggcccg ggcgcggcac ggtgcgcgcc
    14941 acctttcaca tcccggccgg gcgcatcgag gagatccgcg ccgacgccga ccggctgggc
    15001 aaggtcgagc ccaccttcca tgtcgacgtg ctggacgagg agggggaggt ggtcgccgag
    15061 gtcgagaagc tcctctatgt acgaaagaag ggggaggtgc gctaagctac cggcgccgaa
    15121 ccgccgattc gaacgatttc tcgagggacc gccgtatgga ccgccgtgct ctgacctgtc
    15181 tcgccctcct gctcaccctg tcgattccgg ccgccgccga caccctgctg acggtgaaga
    15241 gccatgtcgg cgccttccag ctcatggggg agaaccagcc ggccaaggac accgaagtca
    15301 agatctgggt gggggccgac cggctccgcc gcgacgaggg gcagacctcc tccatcctgc
    15361 ggctcgaccg caacaagctc tacatcgttg accacgccgc caagacctac agcgaggtcg
    15421 gcctgcccgt ggacctgcag aagtacgccc cggccggcac cgaggccgcg atgaagcaga
    15481 tgggcgacgc catgcggctg gacgccaagg tcacccccgc cagcgagacc cgcaagatcg
    15541 gcgactggaa cgcccgccgg gtggacgtgg ccatcaccag cgccatgggc atgaagatcg
    15601 ccaccaccat gtgggtgagc aaggacctgg agatctaccg gtccctcaac cggatgtccg
    15661 ccaccctggc ctccctccag catggcgcgg cggcctgggc ccggcagctc gaggagatcg
    15721 aaggcttccc ggtgctccag gagtccagca tcagcgtggt cggcacccag ttcaagaccc
    15781 gggaggagct ggtcgcagtg gcggcccagg acgcgccggc cgggatctac gagccgccgg
    15841 ccggctatac ccgccagccg ttcaacccca tggcccagcc gggccggtaa gaccccaacg
    15901 gaagagaggg aggagtaggc gcggacgcgc ctactcgcgg tgctgggaga tctggcgccg
    15961 cagcagctcg ttttcgaacg ccatgcccac ctgggcggtg gcgagctcga ggatgtcgat
    16021 gtcctcgatc ggctgatcgc ggtcgccgtt gtccgtgtag atgacggaga tgacccgctg
    16081 cgcgccgagc acggggaaga ccaccacctg gccggtgcgc gggctgccca ccatggagcg
    16141 caggggctcg ggcaggttgg cgtcgtcgaa ggagagggcc tgcacctcgc ccgtctccat
    16201 ggcgcgcgtc agggcgctgt cgctctccag gccgagcttc agggcgcgcg tcacctcggc
    16261 gagcggcttg ccgcgggtgt ccaccccgaa cgcgccgagc gccatcagat ggtcgcgctt
    16321 caccaggaac agcacggcgc gctctaccga ctcggagacg atgtgcatca ggttgagcgc
    16381 cacggtggcc gagatgaggc cggagcggag gtcgccgaag acccgccgg
//
LOCUS       DASPJI010000867        13935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_228199, whole genome shotgun sequence.
ACCESSION   DASPJI010000867 DASPJI010000000
VERSION     DASPJI010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 13935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13935)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13935
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228199"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>13935
                     /locus_tag="VEL74_24400"
     CDS             <1..>13935
                     /locus_tag="VEL74_24400"
                     /inference="COORDINATES: protein motif:HMM:NF004282.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-ribosomal peptide synthase/polyketide
                     synthase"
                     /protein_id="HYG65747.1"
                     /translation="LGTSLQTLIAAAVADPEAPVADLPLLGEPERHQVVLEWNPLRTL
                     RVTEGDGPCIQDLVRRQAARTPEAVAAVYGDRHLTYGELVWRAGALARRLRRLGVGPE
                     VRVGVCLERSLETVVGLLGVLEAGGAYLPLDPTYPQERLAFMIEDGRIAALLTQPSLR
                     DVLPPHALPVVDLRPEGGGAGRDVPRYPHHGATGENLAYVMYTSGSTGRPKGVCIPHR
                     ALHWFVTTLGYVEPGPADRVAQGSTHTFDAATFELFGALVNGARLVGIPKEVMISPRE
                     LAARLEEEGISVLYLTAALFMQTVSEAPAAFQPLRRLLVGGEAVDTAFFRRALESGPP
                     ERLLYLYGPTETTVFSSWYEVALDTLADRVSVGRPIADAPHYLLDAGLRPVPVGAFGE
                     AYIAGGGLARGYLDRPDLTAERFVPSPFSGLVDEPGARMYWTGDLMRYRPDGNLEFGR
                     RVDDQVKIRGFRIEPREIETVLLQHPQVAKALVIVREDAPGDKRLVAYVVAPDDQTPA
                     AGALREFLRERLPDYMVPAAFVPLAALPLFPHGKVDRRALPAPPTADVAPEAGESFAT
                     PRTMTEELLAGIWAEVLGMEKVGLRDDFFELGGHSLLATQVVSRLRSVFQIELPLREL
                     FEEPTVAGLAIRVDRAIRAGAGLLAPPLVPVARQGALPLSFAQQRLWFLDQLTPNGSA
                     YNIPVALRVQGALDIGVLRRSLAEIVRRHEALRTVFAAHDGTPVQAIEPPSDPALLVV
                     DLSGLEEGHREWLSHELAGQEAGRPFDLSRGPLLRAVVVHLAEASHLALLTMHHIVSD
                     GWSMGILVREVSALYQAYAAQRPSPLSELPVQYADYAVWQRSWLQGEVLENELVYWRR
                     QLAGLPPLLELPTDRPRPQVQSFRGAAREVRLSPALTRQVRALARREGATVFMAILAA
                     FQALLARYSGQHDLAVGTPIAGRNRLETEELIGFFVNTLVLRGNVAPGRSFGELLRRL
                     LETALAAHMHQDVPFEKLVEELTPERNLAYSPLFQVMFVLQNAPQESLEMGELRVRSI
                     GAAIAFTKFDLTLTLAETDDLLDGFLGFSTDLFDAPTIDRLLRHFEQLFTAALDAVSA
                     PVEELPLLSQAESRQLLIEWNALQPPFAVPALGVHEIFEQRTAELADRVAVVHGGEQL
                     SFAALNGLANRLAGRLRRSGVGTESYVALVMERGVEVVIGLLAALKAGAAFVIVDPAQ
                     PASRLAQTLADAAPKAVLTRGAAAASLSLHGAAVFRLDGPLDDSESGEDLGLPVVPGQ
                     LAYGIYTSGSTGAPKCVVLRHDAVVNLLLALEREIYAGAAPDLRVAVNTSLHFDGSMK
                     QIIQLLSGRSLDIVPEEIRTDPEVLRAHLAAHQLDVLDCTPSQLRALLEAGLGRDGEV
                     APGRVLVGGEAIEAPLWERLSALAGTRFFNVYGPSECTVDTTVRRITPDAPRPVLGRP
                     IANVGVQVLGPASMPVPGGVPGELCVSGAGLARGYLGRPELTAERFVPDFWGGVPGAR
                     LYRTGDLVRWTAGGELEYLGRIDHQVKVRGFRIELGEIEAALARLAGVREAVVVTREE
                     VSGDRRLVAYVVGAPEGGAWREQLAKRLPEYMVPSALVMLEAMPLTPNGKVDRRALPA
                     PEWRPAEGHLAPRTTVEELLAGIWSEVLGVERPGVEDNFFELGGHSLLATRVMSRLRS
                     AFQVELPLRELFEEPTLAGLAVRVESALRVGAGLLAPPLVPLAPVERQGALPLSFAQQ
                     RLWFLDQFAPNGSAYNLPVALRVQGALDVGVLRRSLEEIERRHEALRTVFIAHEGELA
                     QVIKPALEFVLPVVDLSGLAEGQRERLGSELVGQDAARPFDLARGPLLRARVVRMAEH
                     DHFVLLTMHHILSDGWSMEILVREVTALYVSLTRGTPVSLPELPVQYADYAVWQRSWL
                     QGEVLAREIHHWRGRLAGAPPLLELPADRPRPAVQSQRGAGHTFRLPDPLSERLRALS
                     RQRGSTLFMTLLAGFQALLARSTGQPIILVSTPVAGRNHLALEGLIGFFVNMLVLRAD
                     VAGELSFADLLAQVRETVLAAHAHQDLPFEKLVEELEPERSLSHAPLAQVTFLLQNRP
                     RAELAIENLRLDAVETSTATAKLDLTLALTETGSGFAGALEYSTDLFDGSTVARLAER
                     YRSLLEDAVAQPGRPVADLAQLAGGERHQVLWEWRGGAEAPPPGLPVHALFEEQAALR
                     PAEPALVWDGGSLSYGELDRRADALADHLRGLGVGPEVAVAFCLERSPELVVTTLAIL
                     KAGGAYVPLDPGAPAEWLAWVLSDVQAPVLVTRRTLRDRVPESSAYVVLLDELPATAA
                     TAAPARRSDQTGRTGGDSLAYVMYTSGSTGRPKGVAVVHRAVVRLIRGEFARFGPGET
                     WLQLAPVWFDAATLEIWGALANGAQLAIPPAGALSLAEIGEALGRFGVTTLWLTAGLF
                     RQMVDERPADLGGLRQLLAGGDVLSPAHVRRWLEMVPGGVLINGYGPTENTTFTACGP
                     LAGGGEVGWTVPLGRPITHTRVRILDGALAPVPVGVAGELFAGGEGLARCYLGRPELT
                     AERFIPDPLGEPGARLYRTGDRARWLADGRLDFLGRVDRQVKIRGFRVEPGEVEAVLG
                     RHPAVREAAVVVREDRTGDRRLAAFVVPATSSRQLTEELLDHLRQVLPPYLVPSSLVV
                     LPELPLNSNGKVDRGALLAMRADLTSHGAEEEQGLLTAAEAVMAEIWAEVLGLEQVSP
                     DASFFDLGGHSLLATQVISRVRKVFEVELPMSAVFEDDNVRALAARVDDIRRAERGVR
                     LPDITPADRSDRTLVIPLSFAQQRLWFLDQLVPNSSTYNVPVAWHLSGRLRLSSLAAS
                     LTEVVRRHETLRTTFHPSSAGPFQEIAPPAPVSVPVVDLRALAPVVRDAEAARLMAAE
                     ARRPFDLRRGPLLRSTLLYLEREQALALLTLHHIISDGWSLGVLGREVSTLEEVFAQG
                     LPSPLPELPIQYADFAIWQRRVFSEGALATEVEYWREQLRGAPPIADLPLDHPRPKDL
                     ADDGAVLAHTLPVGLSAALRAFGRRQGATSFITLLAGFQALLGRYAAEPDVVVGTPVA
                     GRTHLEIEGLIGFFVNTLVLRTRMEDGPCFEELVRRVRHTTLEAHAHQDVPFEELVEK
                     LQPERSLAVSPLFQVMFDVKTYTGWRAGLQGLRATMAGVASQTAKFDLNLNVLDLKDG
                     LHLALEYRADLFDATTAWRLLNHLERLLQGAIADPGGRLPELPLLSEAERAQLLEWND
                     TRRSYILERCLHELVEEQARLAPDQTAIVWRDGAVSYGELERRANRLAHELRDLGIGP
                     EDRVGLMLRRSPAMVVGILGILKAGGAYVPLDPSYPDERLAYMIDDAELSVVVTETGA
                     SSVAQASGATVLDLDRERQRIARRSEEAPAPRAVAGNLAYVIYTSGSTGRPKGVMVPH
                     RGVANRLLWAQEVYRLDEQDAVLQKASFSFDFSVWECFAPLLAGARLVLAEPGRQGDG
                     AYLIRTIQEHGVTFVHFVPSMLAVFLAEEGVEECVSLRQVFSGGEALTPELRDRALMR
                     LPAPLDNQYGPTEISIDTTRWVCAPGPDPYRLPVGHPIANSRLYVVDRELRPVAVGLH
                     GELLVGGTGVTRGYLRRPDLTAERFVPDPFAEEAGARLYRTGDLARRLTDGTVEFRGR
                     VDHQVKLRGFRIELGEIESTLAEYPLVRDAVAIVREDVPGDPRLVAYLVADEGAAPRA
                     PELRGFLQELLPEYMIPSAFVVLEAFPRTPAGKVDRRALPAPERTLAQSSALYVAPRT
                     PLEGVLAQVWAELLQAERVGIHDNFFELGGHSLLATQVVSRVRSLLGIEVPLRALFER
                     PTLSGFAYIVESARRSLGGSQMPPLQRVDRSLDLPLSFAQQRLWFLDQLAPGGGAYNV
                     PVALRVEGDLHVGVLQCSLTEIVRRHEALRTVFAAHDGTPVQIVEPPYELALPVVDLS
                     GLEEGRRERLGSELLGREARRPFHLSRGPLLRALVVRLSAEEHLALVTIHHIVSDGWS
                     IGILVREVSMLYASLSARMDSPLPELPVQYADYAVWQRSWLQGEVLAQDIDYWRRQLA
                     GAPALLELPTDRPRPAVQSSRGAAWVFPLPVELSAGLQSLSRRRGATLFMTLLAGFQM
                     LLARYTGQQDVVVGTPIAGRNHVELEGLIGFFINTLILRTDLRGEPGFEDLLGRVRET
                     VLSAHTHQEVPFEKLVEELEPERSLAYSPLFQVLFALQNVPQEELSIRDLRLRPLDSP
                     ATTAKFDLAMALSEAEGEVRGLVEYNTDLFDEVTIERLARHFERLLSAVVEAPSSPVG
                     GLALLSAAELSQLTEWSSGTGDSQRVGICLGELFEAQVDRTPEAVALVWGEEELRYAE
                     LAERSNRLAHYLRRLGVGPERVVGICLERTAELVVAVLGVLKAGGAYLPLDPAYPAQR
                     LSWMLEESAAPVVVTEQRLLASLPPEARRVVCLDAEQAALSAESASRPRPWAVPQNLA
                     YVIYTSGSTGRPKGVAISHRSASLLVQWAGETFGAEELSGVLASTSLSFDLSVFELFM
                     PLSWGGTVILAPNVLHLPALPAAARVRLINTVPSALAELVRAESLPPGVKTVNLAGEP
                     LARGLAQQILASGTVERLWNLYGPSEDTTYSTAAQVEPGEEEASIGRPIPGGQSWVVD
                     GGLRPVPVGVAGELLLGG"
BASE COUNT         1814 a         4572 c         5255 g         2294 t
ORIGIN      
        1 ggctggggac gagcctgcag acgctgatcg ccgccgccgt cgccgatccc gaagcgccgg
       61 tggccgacct gccgctgctc ggcgagcccg agcggcacca ggtcgtgctg gagtggaacc
      121 ctctccgcac cctccgcgtc acggaggggg acgggccctg catccaggac ctggtgcggc
      181 ggcaggccgc gcgcacgccg gaggcggtgg cggcagtcta cggcgatcgt catctcacct
      241 acggcgagct cgtgtggcgg gccggcgcgt tggcgcgtcg cctgcgccgg ttgggggtcg
      301 gccccgaggt gcgggtcggg gtctgcctcg agagatcgct cgagaccgtg gtcgggctcc
      361 tgggcgtcct cgaagcggga ggcgcctacc tgccgctcga tccgacctat ccgcaggagc
      421 gcctggcgtt catgatcgag gacggcagga tcgcggcgct gctgactcag ccgtcgctcc
      481 gggatgtcct gccgccgcac gccctgccgg tggtcgatct gcggccggag ggcggcggcg
      541 cgggccgcga cgtacctcgg tatccacatc acggcgcgac cggcgagaac ctggcttatg
      601 tgatgtacac ctcgggctcg accgggcgcc cgaagggtgt ctgcatcccg caccgggccc
      661 tccactggtt cgtcaccacg ctgggctatg tcgagccggg tccagcggac cgcgtggcgc
      721 agggatcgac gcacaccttc gacgccgcca ccttcgagct cttcggcgcc ctggtgaacg
      781 gcgcgcggct ggtggggatc ccgaaggagg tcatgatctc gccgcgcgag ctggcggcgc
      841 ggctggagga ggaggggatc tcggtcctct atctcaccgc cgcgctcttc atgcagacgg
      901 tgagcgaggc ccctgcggcc ttccagcccc tgcggcggct gctggtcggc ggcgaggccg
      961 tggacaccgc cttctttcgg cgggcgctgg agagcggccc gccggagcgc ctgctctacc
     1021 tctacggccc gaccgagacc acggtcttct cgagctggta cgaggtcgcg ctggacaccc
     1081 tggcagacag ggtctccgtg ggccggccga tcgcggacgc gccgcactat ctcctcgatg
     1141 ccgggctccg gccggtgccg gtcggcgcct tcggcgaggc ctacatcgcc ggcggtgggc
     1201 tggcgcgagg gtacctcgac cggccggacc tgaccgccga gcggttcgtg ccgagcccgt
     1261 tctccggcct cgtcgacgag ccgggggcac ggatgtattg gaccggcgac ctgatgcgct
     1321 accggccgga cggcaacctg gagtttggcc gccgggtcga cgatcaggtg aagatccgcg
     1381 gtttccgtat cgagccgcgg gagatcgaga cggtgctgct gcagcacccg caagtggcca
     1441 aggccctggt gatcgtgcgc gaggacgcgc cgggggacaa gcgtctggtg gcctatgtcg
     1501 tggcgccgga cgaccagacg ccggcggccg gcgcgctacg cgaatttctg cgcgagcgcc
     1561 tgcccgacta catggtgccc gcggccttcg tgccgctcgc ggcactgccc ctcttcccgc
     1621 acggcaaggt ggaccgccgc gcgctgccgg cgccgccgac ggcggacgtg gcgcccgagg
     1681 ccggcgagag cttcgcaacc ccccgcacca tgacggagga gctgctggcc gggatctggg
     1741 ccgaggtcct ggggatggag aaggtcggac tgcgcgacga cttcttcgag ctgggtgggc
     1801 attcgttgct ggcaacgcag gtggtatcac ggctgcgcag cgtgttccag atcgagctgc
     1861 ccctgcgtga gctgttcgag gagcccacgg tggccggtct ggctatccga gtggacaggg
     1921 cgatacgggc gggagccggg ctgttggcgc cgcccctcgt gccggtagcg cggcaaggag
     1981 cgctaccgct gtcgttcgcc cagcagcggc tctggttcct cgaccaactg acgccgaacg
     2041 gctcggccta caacatcccg gtcgcactgc gcgttcaggg cgctctcgat atcggcgtgc
     2101 tgcggcgcag cctggcggag atcgtgcgcc gtcacgaagc gctacgcacg gtgttcgcgg
     2161 cgcacgacgg tacgccggtg caggcgatcg agccgccttc cgaccctgcg ctgttggttg
     2221 tggacttgtc gggcttggag gaggggcacc gcgagtggtt gagccatgag ctggcgggac
     2281 aggaggcggg acgccccttc gatctgtcgc gcggtccgct gctgcgggcc gtggtggtgc
     2341 atctggccga agcgagccat ctggcgctgc tgacgatgca tcacatcgtc agcgacggct
     2401 ggtcgatggg catcctggtg cgggaggtct cggcgctgta tcaggcctat gcagcgcaac
     2461 gtccgtcgcc gctgtccgag ctgccggtgc agtacgccga ctacgcggtg tggcaacgct
     2521 cctggctgca aggcgaggtg ctggaaaacg agctcgtcta ctggcgccgc caacttgccg
     2581 gcctgccgcc gctgctggag ctgccgaccg atcggccgcg gccgcaggtg cagagcttcc
     2641 gtggcgcggc gcgggaggtg cggttgtcgc cggcgctgac ccggcaggtg cgggcgctcg
     2701 cccggcgcga aggggcgacg gtgttcatgg ctatcctcgc cgccttccag gcgctcctcg
     2761 cgcgctacag tggccagcac gatctggcgg taggcacgcc gatcgccggc cgcaaccgcc
     2821 tggagaccga agagctgatc gggttcttcg tcaacacgct ggtgctgcgc ggcaatgttg
     2881 cgcctggacg gtcgttcggc gagctgttga ggcggctgct cgagacggcg ctcgccgctc
     2941 acatgcatca ggacgtgccg ttcgaaaagc tggtggagga gctgacgccg gagcgcaacc
     3001 tcgcctattc gccgcttttc caggtgatgt tcgtgctgca gaacgcgccg caggagagcc
     3061 tcgagatggg agagctgcgg gtgcggtcga tcggggctgc catcgcgttc acgaaattcg
     3121 atctcacgct gacgctcgcc gaaactgacg atctgttgga cggtttcctc ggcttcagca
     3181 ccgacctgtt cgatgccccg acgatcgatc gcctgctccg tcatttcgag cagctcttta
     3241 cggccgccct ggacgcagtg tctgcgcctg tggaggagct gccgctgctg agccaagccg
     3301 agagccggca gctcctgatc gaatggaacg ctctccagcc cccgttcgcg gtgccggcgc
     3361 tcggcgttca cgagatcttc gagcagcgga ccgcggagct cgccgatcgg gtggccgtgg
     3421 tccatggtgg cgagcagctc tccttcgctg ccctgaacgg gcttgcgaac cgtctggcgg
     3481 gccgactgcg ccggtcgggc gtgggcacgg agtcgtacgt cgccctcgtc atggagcgcg
     3541 gcgtcgaggt ggtgatcggt ctgctcgcgg cgctcaaggc gggcgccgcc ttcgtcattg
     3601 tcgatccggc ccagcctgcc tcgcgcctcg cgcagacgct cgccgatgcc gcgccgaagg
     3661 cggttctgac ccgcggcgct gccgccgcga gcctctccct tcacggtgcg gcggtgttcc
     3721 gtctggacgg tcctctggac gacagcgaga gcggcgagga cctcgggctg ccggtcgtgc
     3781 ccggccaact ggcctatggg atctatacct cggggagcac cggagctccc aagtgcgttg
     3841 tcttgcgtca cgacgcggtg gtgaacctgc tgttggccct ggaaagagag atctatgctg
     3901 gcgcggctcc ggacctgcgg gtcgccgtca acacatcgct ccatttcgac ggctcgatga
     3961 agcagatcat ccaactgctc tccgggcgca gtctagacat cgtgcccgag gagatccgca
     4021 ccgaccccga ggtcttgcgc gcgcacctcg cggcccatca gctcgacgtg ctcgactgta
     4081 cgccgtcaca actgcgcgct ctgctcgagg ccggtctggg tcgcgatggg gaggtggcgc
     4141 ccggccgggt cctggtcggc ggcgaggcga tcgaggcgcc gctgtgggaa cgcctgagcg
     4201 cgctggccgg cacgcggttc ttcaatgtct atgggccgag cgagtgcacg gtggacacca
     4261 cggtgcggcg gatcacgccg gatgcgccga ggccagtgtt gggccggccg attgccaatg
     4321 tgggggtcca ggtgctcggc ccggcgtcga tgccggtgcc cggcggggtg cccggggagc
     4381 tctgcgtgag cggcgcgggt ctggcgcgtg gctatctggg gaggccggag ctgaccgcgg
     4441 agcggttcgt gccggatttc tggggcggcg tgcccggggc gcggctgtac cgtacgggcg
     4501 atctcgtgcg ctggacggcc ggcggcgagc tcgagtatct gggccgcatc gaccatcagg
     4561 tcaaggtgcg cgggttccgc atcgagctgg gagagatcga ggcggcgctg gcgcggctcg
     4621 ccggtgtgcg cgaggcggtg gtcgtgacgc gcgaagaggt ctccggcgac cggcgcctgg
     4681 tggcctatgt ggtgggggcg ccggagggcg gagcgtggcg cgagcagttg gccaagaggc
     4741 tgccggagta catggtgccg tcggcgctgg tgatgctgga agcgatgcct ctcacgccga
     4801 atggcaaggt cgaccggcgg gcgctgccgg cgccggagtg gcggcccgcg gaagggcact
     4861 tggcgccgcg tacgacggtc gaggagctgc tggcgggcat ctggtccgag gtgctgggcg
     4921 tggagcggcc gggagtggag gacaacttct tcgagctggg cggtcactcg ctgctggcga
     4981 cgcgggtgat gtcgcggctg cgcagcgcgt tccaggtcga gctgcccctg cgtgagctgt
     5041 tcgaggagcc cacgctggcc ggtctggccg tccgggtgga gagcgcgctg cgggtgggag
     5101 ccgggctgct ggcgccgccg ctcgtgccgc tcgctccggt cgagcggcaa ggagcgttgc
     5161 cgctgtcgtt tgcccagcag cgtctctggt tcctcgacca gttcgcgccg aacggctcgg
     5221 cctacaacct gccggtcgcg ttgcgcgtgc agggcgcgct cgacgtcggc gtgctgcggc
     5281 gcagcctcga ggagatcgag cgccgccacg aggcgttgcg caccgtgttc atcgcgcacg
     5341 aaggggagct ggcgcaggtg atcaagccgg ctcttgagtt cgtgttgccg gtggtggatc
     5401 tgtcgggctt ggcggagggg cagcgcgagc ggctgggaag cgagctggta ggccaggacg
     5461 cggcgaggcc gttcgatctg gcgcgtggcc ctctgttgcg agcgagagtc gtgcggatgg
     5521 cggagcatga ccacttcgtg ctgctgacca tgcaccacat cctcagcgac ggctggtcga
     5581 tggagatcct ggtgcgggag gtgacggccc tctacgtgtc gctcacgaga gggacgcccg
     5641 tctccctgcc ggagctgccg gtgcagtacg ccgactacgc ggtgtggcag cggagctggc
     5701 tgcaaggaga ggtcctggcg cgcgagatcc accactggcg cggacggctc gcgggagcgc
     5761 cccccttgct ggagctgccg gcggaccgcc cccgtcccgc cgtgcagagc cagcgcggcg
     5821 cgggacacac cttcaggctg ccggatcctc tctccgagcg cctccgcgcc ctgtcgcgac
     5881 agcggggctc gacgctgttc atgacgcttc tggcgggctt ccaggcgctg ctggcgcgct
     5941 ctacggggca gccgatcatc ctggtgagca cgccggtggc gggccgcaac cacctggcgc
     6001 ttgaaggcct gatcggcttc tttgtcaaca tgctggtgct gcgggccgac gttgccgggg
     6061 agctgtcgtt cgcggatctg ctggcacagg tccgggagac ggtgctggcg gcgcatgcgc
     6121 accaggacct gccgttcgag aagctggtcg aggagctgga gccggagcgc agcctctccc
     6181 acgcgccgct cgctcaggtg accttcctgc tgcagaaccg gccgcgggcg gagctggcga
     6241 tcgagaatct gcggctggat gcagtcgaga cctcgaccgc gacggcgaag ctcgacctca
     6301 cccttgccct gacggagacc ggcagcggct ttgcgggcgc cctcgagtac agcacggacc
     6361 tcttcgacgg ctcgacggtg gcgcggcttg cggagcgcta ccggtcgctc ctggaagacg
     6421 ccgtggccca gccggggcgg cccgtcgcgg atctggctca gctggccgga ggggagcggc
     6481 accaagtgct ctgggaatgg aggggtgggg ccgaggcgcc gccgccgggt ctgccggtcc
     6541 atgcgctctt cgaggagcag gcggcgctgc ggccggcaga gccggcgctg gtctgggacg
     6601 gcggctcact ctcgtatggg gagctcgacc gccgcgccga cgctctcgcc gatcacctgc
     6661 gcgggctggg agtgggcccg gaggtggcgg tggccttctg cctcgagcgc tcgccggagt
     6721 tggtggtgac gaccctcgcc atcctcaagg cgggcggcgc ctacgtccct ctggacccgg
     6781 gggcgccggc ggagtggctc gcctgggtgc tctccgacgt ccaggcgccg gtcctcgtca
     6841 cccgacggac gctccgcgac agggtgccgg agagcagcgc ctacgtcgtg ctgctcgatg
     6901 agctgcccgc gacggcggcg acggcggcac cggcgcgccg gagcgaccaa accggccgga
     6961 ccggcggcga cagtctggcc tatgtcatgt acacctcggg ctccaccggc cggcccaagg
     7021 gggtggccgt ggtccaccgc gcggtggtcc gcctgatccg gggagagttc gcacgcttcg
     7081 gccccgggga gacctggctc caactcgcgc cggtgtggtt cgacgcggcg acgctcgaga
     7141 tctggggcgc tctggcgaat ggcgcccagc tggccatccc tcccgccggt gccctctcgt
     7201 tggccgagat cggcgaggcc ctggggcgct tcggtgttac tacgctctgg ctcaccgccg
     7261 gcctcttccg ccagatggtc gatgagcggc cggcggacct cggggggctg cgccagctcc
     7321 tcgccggtgg cgatgtgctg tccccggccc acgtccggcg gtggctcgag atggttccgg
     7381 gaggcgtcct catcaacggc tatggcccga cggagaacac gacgttcacc gcctgtggcc
     7441 cgttggccgg cgggggcgag gtcgggtgga ctgtgccgct gggccggccc atcactcaca
     7501 cccgcgtccg catactggac ggcgcgctcg cgccggtgcc ggtgggcgtc gccggcgagc
     7561 tcttcgcagg cggggaggga ctcgcccgct gctatctcgg acggccggag ctgacggcgg
     7621 agaggttcat cccggatccg ctgggggagc cgggcgcgcg cctctaccgc accggcgacc
     7681 gggcgcgctg gctggccgat ggccggctgg acttcctggg gcgtgtcgac cgtcaggtca
     7741 agatccgcgg cttccgcgtc gagcccgggg aggtggaggc ggtcctcggc cgccatccgg
     7801 cggtgcgcga ggcggcggtg gtggtgcgcg aggaccgcac cggcgaccgc cggctggcag
     7861 cattcgtagt gccggccacg agctcccggc aactcaccga agagctgctc gaccacctgc
     7921 gccaggtgct cccgccgtac ctggtgccgt cgtctcttgt cgtcctgccc gagctcccat
     7981 tgaactccaa cggcaaggtg gaccgggggg cgctgctggc gatgcgggcc gacctgacgt
     8041 cccacggcgc cgaggaggag cagggcctcc tcacggcggc cgaagcggtg atggccgaaa
     8101 tctgggccga ggtcctgggg ctcgaacagg tgagtccgga cgccagcttc ttcgacctcg
     8161 gcggccattc gctgctggcg acgcaggtga tctcgcgggt gcgcaaagta ttcgaggtcg
     8221 agctgccgat gagcgctgtc ttcgaggacg acaatgtcag ggcgctggcg gcgcgcgtcg
     8281 acgacatccg ccgagccgag cgaggcgtgc gcctgcccga catcacgccg gccgaccgga
     8341 gcgaccggac cctggtaatc ccgctctcct tcgcgcagca gcggctctgg ttcctggatc
     8401 agttggtgcc gaacagctcc acctacaacg tgccagtcgc ctggcacctg tccgggcgcc
     8461 tccgtctctc ctcgctcgcc gcgagcctga ccgaggtggt ccgccggcac gagaccctgc
     8521 gcaccacgtt ccatccgtcg tccgcaggcc ccttccagga gatcgcgccg ccggcccccg
     8581 tctctgtccc tgtggtggac ttgcgagcgc tggcgccggt ggtccgggat gccgaggcgg
     8641 cgcggctgat ggcggccgag gcgcgacggc cgttcgatct gcggcggggg ccgctcctgc
     8701 gctcgaccct gttgtacctg gagagagagc aggccctggc gctcctcacc ctgcatcaca
     8761 tcatcagcga cggctggtca ttgggggtgc tgggccgcga ggtctccacc ctggaggagg
     8821 tcttcgctca gggcctgccg tcgccgctgc ccgagctgcc catccagtat gccgatttcg
     8881 ccatctggca gcgccgggtc ttctcggaag gggcgctggc gaccgaggtc gagtactggc
     8941 gggagcagct ccgcggcgcc ccgccgatcg ccgacctgcc gctggaccac ccccggccga
     9001 aggacttggc cgacgacgga gcggtcctcg cccacacctt gcccgtcggg ctttctgccg
     9061 cgctccgggc gttcggcagg agacaggggg ccacctcgtt catcactctc ctggccggct
     9121 tccaggcgct gctcggccgg tatgcggcag agccggacgt cgtcgtcggg actccggtgg
     9181 cggggcggac ccatctggag atcgaagggc tgatcgggtt cttcgtcaat acgctggtgt
     9241 tgcggacccg gatggaagac ggcccatgct tcgaggagct cgtgcggcgg gtgcgccaca
     9301 cgacgctcga ggcccacgcg catcaggacg tacccttcga ggagctcgtc gaaaagctcc
     9361 agccggagcg cagcctggcg gtgtcgccgc tcttccaggt gatgttcgac gtgaagacct
     9421 ataccggatg gcgtgcgggc ctgcagggtc tgcgggcgac gatggcgggt gtcgcgagcc
     9481 agaccgcgaa attcgatctc aacctgaatg tcctggatct gaaggatggt ctgcatctcg
     9541 ccctggagta ccgggcggac ctgttcgatg ccaccacggc gtggcgcctg ctgaatcatc
     9601 tggaaaggct cctccagggc gcgatcgccg atccgggggg acggcttccg gagctgccgc
     9661 tcctgtcaga ggccgagcgg gcgcagttgc tggaatggaa cgatacccgg cggtcgtaca
     9721 tcctggagcg ctgcctgcac gagctggtcg aggagcaggc gcggctcgct ccagaccaga
     9781 cggccatcgt ctggcgcgac ggcgcggtga gctatgggga gctggagcgc cgggccaacc
     9841 ggctggccca tgagctgcgc gatctgggca tcggtccgga agaccgggtg ggcctgatgc
     9901 tgcggcgttc gccagccatg gtcgtgggga tcctgggcat cctcaaggcc ggtggcgctt
     9961 acgtcccgct cgacccgtcc taccccgacg agcgtctcgc gtacatgatc gacgacgcgg
    10021 agctctccgt cgtggtcacc gagacggggg cgtcctcggt ggcgcaggcg agcggagcta
    10081 cggtcctcga cctggatcgc gagcgacagc ggatcgcgcg ccggagcgag gaggcacccg
    10141 ctccgcgggc ggtcgccggc aatctcgcct atgtgatcta cacgtccggc tccacgggcc
    10201 ggccgaaagg ggtcatggtc ccccatcggg gcgtggccaa ccggctgctc tgggcccagg
    10261 aggtctaccg gctcgacgag caggatgccg tcctccagaa ggcgtcgttc agtttcgact
    10321 tctcggtctg ggagtgcttc gcgccgctcc tcgccggtgc gcggctggtg ctggccgagc
    10381 cgggccggca gggggacggc gcgtacctga tccgcaccat tcaggagcac ggcgtcacct
    10441 tcgtccactt tgtcccctcc atgctggcgg tattcctggc cgaggagggg gtggaggagt
    10501 gcgtgtcgtt gcggcaggtc ttctcgggcg gggaggcgct cacccccgag ctgcgggacc
    10561 gggctctgat gcgcctcccg gctcccctgg acaaccagta cggcccgacg gagatctcga
    10621 tcgacaccac gcgctgggtc tgcgcgccgg ggccggaccc ctaccgcctg ccggtcggcc
    10681 atcccatcgc gaacagccgt ctgtatgtcg tggaccggga gctgcggccc gtggcggtcg
    10741 gcctgcacgg cgagctgctg gtgggtggga ccggtgtgac gcgaggctat ttgcgccggc
    10801 cggatctcac cgccgaaagg ttcgtacccg atccgttcgc ggaggaagcc ggcgcgcgtc
    10861 tgtaccggac gggtgatctg gcgcgccgct tgacggatgg cactgtggag ttccgggggc
    10921 gcgtcgatca ccaggtcaag ctgcgcggat tccggatcga gctcggcgag atcgagtcga
    10981 ccctggccga atatcccctg gtgcgggacg ccgtcgcgat cgtgcgggag gacgtcccgg
    11041 gcgacccacg gctggtggcc tatctcgtag cggacgaagg ggccgctcca agggcgccgg
    11101 agctgcgcgg cttcctgcag gagctccttc cggagtacat gatcccgtcc gcattcgtcg
    11161 tgctggaggc gtttccgcgc acgcccgccg gcaaggtgga ccgccgggct ctgccggccc
    11221 cggagaggac gctcgcgcag tcctcggccc tctatgtcgc gccgcgcacc cccctggagg
    11281 gggtgctggc gcaggtctgg gcggagctgc tgcaggccga gcgggtgggg attcacgaca
    11341 acttcttcga gcttggcggt cattcgctcc tcgccaccca ggtggtctcc cgcgtccgga
    11401 gtcttctggg gatcgaggtg ccgttgcgcg cactgttcga gcggccgacc ctgtccggct
    11461 tcgcctatat cgtcgagtcg gcgcgacgga gcctcggcgg ctcgcagatg ccgccgctcc
    11521 agcgcgtcga ccgcagcctc gacctcccgc tctcctttgc ccaacagcgg ctctggttcc
    11581 tcgaccagct cgcgccgggg ggaggggcct acaacgtccc cgtcgccttg cgggtcgagg
    11641 gcgatctgca cgttggcgtg ttgcagtgca gcctgacgga gatcgtgcgg cgtcacgaag
    11701 cgctgcgcac ggtgttcgcg gcgcacgacg gcacgccggt gcagatcgtc gagccgcctt
    11761 acgagctggc gctgccggtg gtggacctgt ccggcctgga ggagggtcgt cgcgagcggc
    11821 tgggaagcga gctgttgggc cgggaggcga ggaggccctt ccatctgtcg cgcggcccgc
    11881 tgctgcgggc tctagtggtg cgtctgagcg cagaagagca cttggcactg gtgacgatcc
    11941 atcacatcgt cagtgacggc tggtcgatag gcatcctggt gcgcgaggtc tcgatgctgt
    12001 acgcgtcgct gtcggcgagg atggactcgc cgctgccgga gctgccggtg caatacgccg
    12061 actacgcggt gtggcagagg agctggttgc agggtgaggt cctggcgcag gatatcgact
    12121 actggcgccg gcagctcgcg ggtgcaccgg cgctcctgga gctgccgacg gaccgcccgc
    12181 gcccggccgt gcagagctcg cgcggcgcgg cgtgggtctt ccctcttccc gtcgagctct
    12241 cagcgggcct gcagagtctg tcgcgccgcc ggggcgcgac gctgttcatg acgctgttgg
    12301 cggggttcca gatgcttctg gcgcgctaca ccgggcagca ggacgtggtg gtgggcacgc
    12361 cgatcgccgg ccgcaaccac gtcgagctcg aagggctgat cggcttcttc atcaacacgc
    12421 tgatcttgcg caccgacttg aggggagagc cggggtttga ggacctcctg ggccgggtac
    12481 gcgagacggt gctgtcggcg catacgcacc aggaggtgcc gttcgaaaag ctggtcgaag
    12541 agctcgagcc agagaggagc ctggcgtact cgccgctgtt ccaggtgctg ttcgcgctgc
    12601 agaacgtgcc tcaggaagag ctgtcgatcc gggatctgcg cctgcgcccg ctggactcgc
    12661 cggcgacgac cgccaagttt gacctggcga tggcgctgag cgaagcggag ggcgaggtga
    12721 gaggtctggt ggagtacaac acggacctct tcgacgaggt gacgatcgag cgtctggcca
    12781 ggcatttcga acggttgctc tcggccgtgg tggaggcgcc gtcgtcgccg gtagggggct
    12841 tggcgctgct gagcgcagcc gagctgtcac agctcaccga gtggagcagc gggaccggcg
    12901 attctcagag agtcgggatc tgcctgggag agctgttcga ggcgcaggtg gaccggacgc
    12961 ctgaggcggt ggcgctggtg tggggcgaag aggagctgcg ctatgcggag ctggcggagc
    13021 gcagcaaccg cctggcacac tacctgcgcc ggctgggcgt cggtccggag cgggtggtcg
    13081 ggatctgtct ggagcggacg gcggagctgg tggtggcggt gctgggcgtc ctgaaagcgg
    13141 gaggagccta cctgccgctg gacccggcct acccggcgca gcggctgtcc tggatgctgg
    13201 aagagtcggc ggccccggtg gtggtgaccg agcaacggct cctggcgagc ctgccgcccg
    13261 aggcgcggcg ggtggtctgc ctggatgccg agcaagcggc gttgtcggcc gagagcgcct
    13321 cccgcccgcg gccttgggcc gtcccgcaga acctggccta tgtgatctat acgtcgggct
    13381 cgacagggcg gccgaagggg gtggcgatct cccatcgcag tgcgtcgctg ctggtgcagt
    13441 gggccggtga gaccttcggc gccgaagagc tgtcaggggt gctggcttcg acttcgttga
    13501 gcttcgacct gtcggtgttc gagctgttca tgccgctgtc gtggggtggc actgtgatcc
    13561 tggcgccaaa cgtgttgcac ctgccggctc tgccggcggc ggcgcgggtg cgcctgatca
    13621 atacggtgcc ttcggcgctg gcggagctgg tgcgagccga gtcgttgccg ccgggtgtga
    13681 agacggtcaa cctcgccggc gagccgttgg cgcgcggtct ggcgcagcag atcctggcga
    13741 gcggcaccgt cgagcggctg tggaacctgt acggtccgtc ggaggacacg acatattcga
    13801 ccgccgcgca ggtcgagccc ggggaggagg aggcgtcgat cggccggccg atccccgggg
    13861 ggcagtcgtg ggtggtggac ggcggtctga ggccggtgcc ggtgggagtg gcgggagagc
    13921 tgcttctcgg gggta
//
LOCUS       DASPJI010000868         8255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_166372, whole genome shotgun sequence.
ACCESSION   DASPJI010000868 DASPJI010000000
VERSION     DASPJI010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8255)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8255
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166372"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VEL74_24405"
     CDS             <1..413
                     /locus_tag="VEL74_24405"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HYG65748.1"
                     /translation="ASGSPGHDRPDLGTVYAEPTNDIERAIAGHMRQLFGIDRIGIHD
                     DFFELGGHSLMAVQLVSRLRSDLQVDLSLSSVFEAPTVSRLAARVAELRLDGMEDAGD
                     IEELLREIEEMSADDLRREIARELGAAGEEAAHG"
     gene            406..1671
                     /locus_tag="VEL74_24410"
     CDS             406..1671
                     /locus_tag="VEL74_24410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MupA/Atu3671 family FMN-dependent
                     luciferase-like monooxygenase"
                     /protein_id="HYG65749.1"
                     /translation="MDKLSRRVAALSPEQRELLALRARRVLPDVVIPGDPGTAVPSAA
                     ASSGPAVSLAPLAGGAGRAAAQEIRFSLLFFSAAGDRDGEGKYQLLLESARFADRHGF
                     EAIWTPERHFQKFGGLYPNPAVLSAALAMVTERLEIRAGSLVLPLQNPIRVVEDWALL
                     DNLSGGRVAVSFASGWNPVDFALAPEAYEERRRLMFEGIETVRRMWAEGRARVPGVGG
                     KEVEVALRPRPLRPELPVWLTSSGTLETWLKAGEIGANVLCALQGEPAEDMAHRIRLY
                     RQARREHGHDPGTGRVTVMLHTYLGDELAAVRETVRGPMTGYLRTFLAQGRSLEGGLS
                     GIDPRQLSDRDLTDLAGAVFERFFEERSLLGTPETCERLLSLLQSIGVDEIACLVDFG
                     LDPEVVLDGLERLAALKRRHEQGASREEA"
     gene            1674..>8255
                     /locus_tag="VEL74_24415"
     CDS             1674..>8255
                     /locus_tag="VEL74_24415"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65750.1"
                     /translation="MDLSSRIATLTPDQRRLFNLRLRHVKPQLLKLQPIPRRDGDGPA
                     LLSYEQERLWFIQQLFPDAAGFNISTIFHFREALDIAAVASSFAEVVRRHEILRTAFV
                     VIDGEPRQVVAPEARLRLAEVDLRGLSESRRVDEMMRWLGPLARRPFNLAAGEPLRIA
                     LFHTGDEAATLLLTVHHIVTDWWSFNLIEHELAALYEDYRHGRPSSLPPLPIQFADFA
                     IWQREWMRGEAFTRQLEFWRRQLAGAPTQLELPADRPRPATPSFRGARQPVRLARGVV
                     ETLKVVDHREESSLFSSVATGLAVLLSRWSGQEEILFGTPVSDRSRPETEALIGYFLN
                     LLILRVDVRGRPAFRQLVERVRGVVQEAYANQDLPFGKLLDDLGGERTLNRNPLFQVA
                     YVYIKNPDPASAPPETSPDGVPVFDPGTTRVDMTLVFEERALGIHSFIEYSREIFDTT
                     TIARLAGQLERLLAGAAADPGRASDDLELLSVAERWCLVGEWNDTVVPRPRDLFAHQL
                     VMDHGRRAPDAVALTFGDQCLTYGELARRSGRLARRLRRVGVGRGSLVGLGSARSPEL
                     IVTLLAVLEAGGAYLPLDLSYPRERLALMLGATGAAFLLIGGGEADAFQGLVSSPLRT
                     VHLDEGDEEDTILHGDGAEPVARLDPDDLAYVLFTSGSTGRPKGVEIPHGALLNFLLA
                     ARRTLGLTDRDVLLAISSLSFDASVLDLFLPLIVGARMDLLAREEVTDGTALARRLEE
                     NGATVLLATAGPWRMLLDAGWQGGHLRALTGGEVLSLPLAAALGERVGELWNIYGPTE
                     TTVASTFWRVELDDQGPVPLGRPLDNTRIHVLDAEMRPVPAGVAGELYIGGTGVARGY
                     RGRPDLTAERFVPDPMGTPGGRLYRTGDRGCRRCDGVLAFHGRLDHQIKVRGYRIEPG
                     EVEAALLLHPAVGEAAVLAAAGDSGLTSLVAWVAPPPGMKSPVAGELRDFLTERLPAQ
                     MVPSLYAFLEALPRTPVGKVDRRELASRKARAEPAAARTAMRTPTEEVVAALWTSLLG
                     IPHVGADDDFFLTGGNSLLGFQLVVRVRQALGVELPLRALFETPSVAQLAARIDCERR
                     RGQSIELPPLAPLSRPDEIPLSFAQQRLWFIEQLEPGSAVYNTPFFLRLTGALDVVAL
                     ASAFAGVVERHEVLRTTFVAAEGNPAVQVIGSRYAPGLPVIDLGDLVTMSSEAEARRL
                     AALEAARPFDLRCGPVLRTTLLRLAEAEHVLVVNAHHIATDAWSLEILWREVAALYDG
                     FRQGRPAALPPLPVQYADFAIWQRGWLGGKVLEAQLGYWRSRLGDLPVVELPVDRPRP
                     AVQSYRGADLPLALPLDASVALSALSRREGATPFMTLLALFVALLGRITGQRDLPVGT
                     PISGRRELEVEGLVGFFLNTLVIRAGLPEGITFRRLVGQLRGLALEAYSHQDLPFEKL
                     VDELSPQRSLSHSPLFQVFFVLLHQTAGTEVTPEGLAVAPFQVEREISRFDLMLSLDA
                     GGEAFSGTLEYATDLFDRPTIARLAAHLGTLAAAVTAAPDGELSALPLFAAAERHQLL
                     MEWNDTARPLPEPSLYQSMERQAERAPDAVALRFGDHWLSYGELALRSARLARCLWRL
                     GVGPESRVGVGLDRSPEMAMGVLGVLAIGAAYVPVDPELPRERRDRMLEDARVEILLT
                     RRGLLAEMSREGVRSVCLDLPIPEGPEFPPGGPPTDPDCLAYILFTSGSTGTPKGVAL
                     PRRALENLIDWHLRHSLGGARTLQIASLSFDVSFLEMFACWGSGGTLLLLSEELRRDV
                     PALADFLVEAEAEKAMFPVVLLQRLAEEYAGRGFRSLPPLAEIISLGEQLQTSRVMGE
                     LLRNLRSCTFYNYYGPSESHLATAYTLDADPAGWVTHPPIGLPIANTRVHLLDDRFAP
                     VPVGVPGELAIGGVCLARGYAGRPDLTAERFIPDPFADVIDEPGSRLYLTGDKVRRLP
                     GGPLDFLGRFDHQVKVRGFRVEPEEIESVLGGCPGVRQAVVLARQVSPGDTRLIAFLV
                     SEPGGVGAPGIWRALLREKLPDYMVPSHFVILDALPLNANGKVDRRALLAHGAALAET
                     ERPDLAATYLGPCTAIEETLAAIWGDVLGIERVGVDDDFFELGGHSLLATQAVSRVRQ
                     ALAVDMPLRTLFKNQTVRAFAKQVEELRREKSGQIVPPLRPAPRGDRIPLSFAQQRLW
                     FIDQ"
BASE COUNT         1161 a         2776 c         2866 g         1452 t
ORIGIN      
        1 ctgcgtctgg ctcgccgggg cacgaccggc ccgatctggg gaccgtctac gccgagccca
       61 ctaacgacat cgagcgcgcc attgccggcc acatgcggca gctcttcggc atcgaccgga
      121 tcggcatcca cgacgacttc ttcgagctgg gcggccactc gctcatggct gtccagctcg
      181 tgagccggtt gcgcagcgat ctgcaggtcg acctctcgct gagcagtgtc ttcgaggcgc
      241 cgacggtgag ccggctcgcc gccagagtgg ccgagctgcg cctggacggc atggaggacg
      301 cgggcgacat cgaggagctg ctgcgggaga tcgaagagat gtcagcggat gacctacggc
      361 gcgagattgc gcgcgagctt ggcgcggccg gtgaggaggc ggcgcatgga taagctgtcg
      421 cggcgcgtcg cggcgctgtc gccggagcag cgggagctcc tggctttgcg ggcgcggcgg
      481 gtgctcccgg acgtggtgat cccgggcgat cctgggacgg ccgtaccgtc tgcggccgcg
      541 tcttccggac cggcggtctc cctggcacca ctggccggtg gggccggcag ggccgccgcc
      601 caggagatcc gcttcagtct cctcttcttt tctgccgcgg gggaccgcga tggcgaaggc
      661 aagtaccagc tgctgctgga gagtgcgcgc ttcgccgacc gccacggctt cgaggcgatc
      721 tggacgccgg agcggcactt ccaaaagttc ggcgggctgt acccgaaccc ggcggtgctc
      781 tcggcggcgc tggccatggt caccgagcgc ctggagatcc gtgccggcag tctggtgctg
      841 ccgctgcaga acccgatccg ggtggtggag gactgggccc ttctcgacaa cctgagcggc
      901 gggcgggtgg cggtctcctt cgcctccggc tggaacccgg tggacttcgc cctggccccc
      961 gaggcctacg aggagcgccg ccggttgatg ttcgagggta tcgagacggt ccgccgcatg
     1021 tgggccgagg ggcgggcccg ggtgccgggc gtgggaggga aggaggtgga ggtcgccctg
     1081 aggccgcgtc cgttgcggcc ggagctgccg gtctggttga cttcgtcggg gacgctcgag
     1141 acttggctca aagccgggga gatcggcgcc aatgtcctct gcgccctcca gggcgagccc
     1201 gcggaggaca tggcgcaccg catccggctg tatcgccagg cgcggcgcga gcacggccac
     1261 gacccgggca ccgggcgggt gacggtaatg ctccatacct atctgggaga cgagctcgcg
     1321 gccgtgcgcg agacggtgcg cggccccatg accggatacc tcaggacctt tctcgctcag
     1381 ggccgcagcc tggagggtgg gctctcgggc atcgacccgc gccagctctc cgaccgcgat
     1441 ctgaccgacc tcgccggcgc cgtgttcgag cgcttcttcg aggagcgctc gctcctcggt
     1501 accccggaga cctgcgagcg tctcctctcc ctcctccaga gcatcggggt cgacgagatc
     1561 gcctgcctgg tggatttcgg cctcgatccc gaggtggttc tggacggcct agagcgcctg
     1621 gcagccctca agcggcgcca tgagcagggt gccagcaggg aggaggcgtg acgatggatc
     1681 tgtcctcccg tatcgccacc ctcacaccgg atcagcggcg cctgttcaac ctgcgattgc
     1741 gccacgtcaa accgcagttg ctgaagctcc agcccattcc acggcgggat ggcgacgggc
     1801 cggctctcct gagttacgag caggagcggc tctggttcat ccaacagctc tttcccgacg
     1861 ccgccggctt caacatcagc accatcttcc acttccggga ggcgctggac atagcggccg
     1921 tagcctcaag ctttgccgag gtggtgcggc gtcacgagat cctgcgcacc gctttcgttg
     1981 taatcgatgg cgagccgcgg caggtggtgg cgccggaggc gcgtctgcgt ctagcggagg
     2041 tggacctgcg ggggctttcg gaatctcggc gcgtcgacga gatgatgcga tggctcgggc
     2101 cgctggcccg tcgccctttc aatctcgcgg cgggggaacc gctacgcatc gcactgttcc
     2161 atacaggcga cgaggcggcc accctgctcc tgaccgtgca ccacatcgtt accgactggt
     2221 ggtccttcaa tctcatcgag cacgagttgg cagcccttta cgaagactac cgccatggcc
     2281 ggccgtcctc gctgccgccg ctgcccattc agtttgccga cttcgcgatc tggcagcggg
     2341 agtggatgcg gggggaggcc ttcactcgcc aacttgagtt ctggcggcgg caactcgctg
     2401 gtgcccccac tcagctcgag ctgccggcgg accgaccgcg ccccgcgacc ccgagcttcc
     2461 gcggcgctcg ccagcctgtc cggttggcgc ggggtgtggt ggagacgctg aaggtcgtgg
     2521 atcaccgtga agagtccagc ctcttctcct cggtggcgac cggcctggca gtgcttctgt
     2581 cccggtggtc cggacaagag gagattctct tcggcacccc cgtctccgac cgcagccggc
     2641 cggagactga ggcgctcatc ggctacttcc tcaacctgct gatcctgcgc gtcgatgttc
     2701 gcggcaggcc ggcgttccgg caacttgtgg agcgggttcg cggagtggtc caggaggcat
     2761 acgccaacca ggaccttcct ttcggcaagc tgctggacga cctcggaggc gagaggacac
     2821 tcaaccgcaa ccccctcttc caagtggcct acgtctatat caagaacccg gatcccgctt
     2881 cagcgccgcc ggagacgtcg cctgacggcg tccctgtctt cgatcccggc accacccggg
     2941 tcgacatgac cctggtgttt gaggaacggg ctctcggcat ccactctttc atcgaataca
     3001 gccgcgagat cttcgacact acgactatcg cccgtctcgc cggtcagctc gagcggctcc
     3061 tggccggcgc ggccgccgac cccggccgcg ccagcgacga cctggagctg ctctcagtgg
     3121 cggaacgctg gtgcctggtg ggggagtgga acgacaccgt cgtgcctcgg ccgcgggatc
     3181 ttttcgccca ccagttggtg atggatcatg ggagacgcgc tccggacgcg gtggcgctca
     3241 ctttcggcga ccagtgcctg acctacggag agctggcccg gcggagcggc cgcttggctc
     3301 ggcggctccg ccgcgtcggc gtcgggcgcg ggagcttggt ggggctgggg agtgcccgct
     3361 cgccggaact gatcgtcacc ctcctggcgg tgctcgaggc ggggggggca tatcttccgc
     3421 tggacctctc ttatccgcgg gagcgcctgg ctctgatgct cggggcgacc ggtgccgcct
     3481 tcctgctgat cggagggggg gaggctgacg ctttccaggg cctcgtctcc agtccgctcc
     3541 gcacggtgca cctcgatgag ggtgacgaag aggacaccat cctccacggt gacggcgcgg
     3601 agcccgtcgc ccggctcgat ccggatgatc tggcctacgt gctttttacc tcgggctcga
     3661 caggccgtcc aaaaggggtg gagattcctc atggagcgct cctcaacttc ctgctagctg
     3721 cgcggcgaac ccttggactg accgaccgcg atgttcttct cgcgatcagc tccctctcgt
     3781 tcgacgcttc ggtgctggac ctcttcctgc cgctgatcgt gggggcacgg atggacctcc
     3841 tcgctcgcga ggaagtgaca gacggcacgg cgttggcccg acggctcgag gaaaacgggg
     3901 ctacggtgct gctggctacc gccggcccct ggcgcatgct cctcgacgcc ggttggcagg
     3961 gtggtcacct gcgagcgctg acaggtggcg aggtcctgtc actgccgctg gctgcggcgc
     4021 tcggggaacg ggtgggtgag ttgtggaaca tctacggccc gacggagacc acggtggctt
     4081 ccaccttttg gcgggtggag ctggacgacc agggcccggt gccgctcggc cgcccattgg
     4141 acaatacccg gatccatgtg cttgacgccg agatgcggcc ggtacccgca ggggtcgcgg
     4201 gtgagctcta tatcggtggc actggcgtcg cgcgaggtta ccgcggccgc cccgacctga
     4261 ccgccgagcg cttcgtgccc gacccgatgg ggaccccggg tggacggctc taccgcaccg
     4321 gcgaccgggg ctgcagaagg tgcgacggcg tgctggcgtt ccacggccgc ctggaccacc
     4381 agatcaaagt acgggggtac cgcatcgagc cgggagaggt ggaggcggcg ctactacttc
     4441 atcccgcggt gggggaagcc gccgtactgg cggcggcggg agacagtggc ctgaccagcc
     4501 tggtggcctg ggtcgcgccg ccgccaggga tgaagtctcc cgtagccggc gaactgcggg
     4561 acttcctgac ggagcggctc ccggcacaga tggtcccgtc gctttacgcc ttccttgagg
     4621 cgctgccccg aacgccggtg ggaaaggtgg atcgccggga gctggcgagt cgcaaggcaa
     4681 gagccgagcc ggcggcggca cggaccgcca tgcgcactcc caccgaagag gtggtggccg
     4741 ccctttggac ctcgcttctc ggcattcccc atgtgggggc ggatgacgac ttcttcctga
     4801 ccggcggcaa ttcgctcctg gggttccaac tcgtggtgcg ggtgcggcag gccttgggag
     4861 tggagttgcc cctgcgcgcc ctgttcgaga cgccctccgt ggcgcagctt gccgcccgga
     4921 ttgactgtga gcggcgacgt ggccagtcga tcgagctgcc gccgctcgcg ccactctccc
     4981 ggccggacga gatccctctg tcgttcgccc agcagcggct ctggttcatc gagcaactcg
     5041 agccgggcag tgccgtctac aacaccccct tcttcctgcg gctcaccggc gcgttggacg
     5101 tggtggcgct ggcctcggcc tttgccggtg tggtcgaaag acacgaggtc ctacgcacga
     5161 ccttcgtcgc cgccgagggg aatcccgccg tgcaggtcat tggttcccgc tacgctccag
     5221 gcctgccggt gatcgacctg ggagacctcg tcacgatgag cagcgaggct gaggcacgcc
     5281 ggctggcggc gctcgaggcc gcgcgcccct tcgacttgcg gtgcggcccg gtcttgcgga
     5341 ctaccctgct gcgtctggcc gaagccgagc atgtcctcgt ggtcaacgcc caccacatcg
     5401 ccactgacgc atggtctttg gagatcctct ggcgggaggt ggcggcgctc tatgatgggt
     5461 tccgccaggg gcgaccggcc gccttgccac ctttgccggt acagtacgcg gatttcgcga
     5521 tttggcagcg cggctggctt ggcggaaagg tgttagaggc ccagctcggc tattggcgtt
     5581 cgcgcctcgg cgacctgccg gtggtggagc tgccggtgga ccggccgcgc ccggcagtgc
     5641 agagctatcg cggcgccgac ctgcctctgg ccttgccgct ggacgcctct gtggcgctga
     5701 gcgcgctgtc ccggcgcgaa ggggctaccc ccttcatgac cctgctggcg cttttcgtcg
     5761 ccctcctcgg ccgcatcacg gggcagcggg acctgccagt gggtacaccg atctcgggcc
     5821 gccgcgagct ggaggtcgag gggctggtcg gcttcttcct gaacaccctg gtgatccggg
     5881 cagggcttcc ggaggggatc accttccgca ggttggtcgg acagttgcga ggtttggcgc
     5941 tcgaggccta ctcccaccag gatctgccgt tcgaaaagct agtggacgag ctaagcccgc
     6001 aacgcagcct tagccactcg ccgctcttcc aggtgttttt cgtccttctc catcaaacgg
     6061 caggtaccga ggtcacgccg gaggggctgg cggtcgcccc cttccaggtg gaacgagaga
     6121 tctccaggtt cgatctcatg ctctccctcg acgccggagg agaggccttc agcggcaccc
     6181 tcgagtacgc caccgacctt ttcgaccggc cgaccatcgc ccgtcttgcg gcacacctgg
     6241 gaaccctggc cgcggcggtt acggcggcac cagatgggga gctctcggct ctccccttgt
     6301 tcgccgcagc cgaacggcac cagctcctga tggagtggaa cgacaccgct cgcccgttgc
     6361 cggagccatc gctttaccaa tccatggagc gtcaagcgga gcgggcaccc gacgcggtgg
     6421 cgctgcgttt cggcgatcac tggctgagct acggggaact ggcgctccgg tcggcacggc
     6481 tcgcccgctg tctgtggcgg ctgggggtcg gcccggagtc gcgggtggga gtgggcctgg
     6541 accgctcacc ggagatggca atgggcgtcc tcggtgttct cgccataggg gccgcctacg
     6601 tgccggtcga ccccgagctc ccccgggaga ggcgggacag gatgctggaa gacgcccgtg
     6661 tggagatcct gctgacgcgg cgggggctgc tcgccgaaat gtcccgagag ggggtccgga
     6721 gcgtctgtct cgacctgccc attccggagg ggccggagtt tccgcctggc ggtccgccga
     6781 cggatcccga ctgcctcgcc tacatcctct ttacctcggg ctctacgggc acgcccaaag
     6841 gggtggcgct gccgcgccgt gctctggaga accttatcga ctggcacctc cggcattcat
     6901 tgggcggtgc gcgaaccctg cagatcgcgt cgctgagctt cgacgtgagc ttcctcgaga
     6961 tgttcgcctg ctggggctcg ggcggcaccc tcctcctcct gtctgaagag ctgcggcggg
     7021 atgtgccggc ccttgcagac ttcctggtcg aagcggaggc cgagaaggca atgttcccgg
     7081 tggtcctcct acagcggttg gccgaggagt acgcggggcg cggcttccgc tcgctgccgc
     7141 cgctcgcgga gatcatcagc ctgggcgaac agctccagac atcccgggtc atgggagagc
     7201 tgctccggaa cctccgaagc tgcaccttct acaactacta cggcccctcc gagagccacc
     7261 tggccaccgc gtacaccttg gacgccgatc ctgctggctg ggtgacccac ccacctatcg
     7321 gtctgcccat cgccaacacg cgcgtccatc tgctcgatga ccggttcgcg cccgtgccgg
     7381 tgggcgttcc cggtgagttg gcgatcggcg gcgtctgcct cgcgcgggga tatgccggac
     7441 ggcccgacct gacggcggag aggttcatcc cagatccctt cgccgacgtt atcgacgagc
     7501 ccgggagccg cctctatctg accggcgaca aggtccgccg ccttcccggc ggccctctcg
     7561 acttcctggg ccgtttcgat caccaggtga aggtgcgcgg cttccgcgtc gagccggagg
     7621 aaatcgagtc ggtgctcggc ggctgtcctg gtgtgcggca ggccgtcgtg ctggcgcgcc
     7681 aagtctcccc tggggatacc cgtctgatcg cctttttggt ctcggaaccg gggggagtgg
     7741 gtgcaccggg gatctggcgc gccttgcttc gagagaagct gccggactat atggtccctt
     7801 cccacttcgt catcttggat gccctgccgc tcaacgccaa cggcaaagtg gaccgccggg
     7861 ccctcctcgc gcacggtgcg gcactggccg agacggagcg gccggacctc gcggcgacct
     7921 atctgggacc gtgtacggcg atcgaggaga ccctggcggc gatctggggc gacgtgctgg
     7981 gcatcgagag ggttggagtc gatgacgact tcttcgagtt gggcgggcac tccttgctcg
     8041 ccacccaggc ggtgtcgcgg gtccggcagg ccctcgccgt cgacatgccg ctacggacac
     8101 tgttcaagaa ccaaaccgtg cgcgcgtttg ccaagcaggt cgaagagctc agacgggaaa
     8161 agagcgggca gatcgtaccg cccctgaggc cggcgccgag aggcgaccgg atccccctct
     8221 ccttcgccca gcagcggctc tggttcatcg accag
//
LOCUS       DASPJI010000869         3247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_166592, whole genome shotgun sequence.
ACCESSION   DASPJI010000869 DASPJI010000000
VERSION     DASPJI010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166592"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VEL74_24420"
     CDS             <1..578
                     /locus_tag="VEL74_24420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65751.1"
                     /translation="SEPAAAPRPVPVPSSAAAVPAVDTVGGPFRKMTVEQSVGVPWSQ
                     RLVPPLLGAGLALLLAATLLSSPVSLLLPFPWQDRERALVTETQRASLYLKVDRAAKT
                     HFLLEGSFPDQLSRLAGLGLLSSQDLRDSQGRLLQYTPGEESYTLQPLANGAPVAGEG
                     ATEAITGNFLLDPEFLKMAPQTKGQPLILLD"
     gene            773..2686
                     /gene="dnaK"
                     /locus_tag="VEL74_24425"
     CDS             773..2686
                     /gene="dnaK"
                     /locus_tag="VEL74_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HYG65752.1"
                     /translation="MSKIIGIDLGTTNSVVAVMEGGEARVIPNAEGNRTTPSVVAFTK
                     SGERLVGQVAKRQSVTNPKNTIFSIKRFMGRKHDEVSEEMKMVPYQIERAENGDVRVT
                     VEGKKYSPPEISAMILGKLKQAAEDYLGEKVERAVITVPAYFNDSQRQATKDAGRIAG
                     LTVDRLVNEPTAAALAYGLDKKKDQTIAVYDFGGGTFDISILEVGEGVVEVKATNGDT
                     HLGGDNIDQRIIDWLLEEFKKDQGIDLGNDPMAIQRLKEAAEKAKIELSSAQETDINL
                     PFITADASGPKHMNLRLTRSKLEALVDDLIQRSIGPCRQAIKDAGIDPKEIDEVVLVG
                     GQTRMPAIQKLVKDFFGKEPHKGVNPDEVVAVGAAIQGGVLSGDVKDMLLLDVTPLSL
                     GIETLGGVFTKLIDRNTTIPTRKSEIFSTAADNQTSVEVHVLQGEREMARDNRTLGKF
                     HLVGIPPAPRGMPQVEVTFDIDANGIAHVSAKDLGTGKEQKITITASSGLSDSDIDRM
                     VREAESHRGEDQERRRQVEARNRLDGLVYQTEKTYDEHKEKLGPDEKGELERALAEAK
                     SSLEGEDTGAMERAAERLTQASHKLAEAMYRQQQQGGGAGAQGGPGAGAGGQQQGSSD
                     EVIDAEYVDVDKS"
     gene            2697..3146
                     /locus_tag="VEL74_24430"
     CDS             2697..3146
                     /locus_tag="VEL74_24430"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HYG65753.1"
                     /translation="MAPLKQIYLEELARIQDRINSLFEQALVTSEYEDREGGLPGTWA
                     PSVDVLETEDSYMVFAELPGVRRDDIQLHIQDRRLEISGRRQSPGENRNFLRMERSYG
                     PFRRIFDLGAPVDADGVTAAFRQGVLRVHIPKRRGGATTVPVREEGA"
BASE COUNT          593 a         1086 c         1124 g          444 t
ORIGIN      
        1 gctccgagcc ggccgccgct ccgcgtccgg tgcccgtgcc gtcatccgcc gcggcggtcc
       61 cggccgtcga caccgtgggc ggcccgttcc gcaagatgac cgtcgagcag tcggtgggcg
      121 tcccctggtc ccagaggctg gtgccgccgc tcctcggcgc cgggctcgcc ctcctgctcg
      181 ccgccacgct gctgtccagc ccggtgtccc tgctgctgcc tttcccctgg caggaccggg
      241 agcgggcgct ggtcaccgag acccagcggg cctctctcta tctgaaggtg gaccgcgccg
      301 ccaagaccca cttcctgctc gagggaagct tccccgacca gctgagccgc ctcgccgggc
      361 tgggtctcct gtcctcgcag gacctccgcg actcccaggg ccggctcctc cagtacaccc
      421 cgggcgagga gagctatacc ctccagccgc tcgcgaacgg cgctccggtg gcgggggagg
      481 gggcgaccga ggccatcacc ggcaacttcc ttctcgatcc cgagttcctg aagatggctc
      541 cccagaccaa ggggcagcct ctgattctcc tcgactgatc cctctcctcg ccgtgcgggc
      601 cccggaccgc cggccctggc tccggccgga ataaatctgc gtccgatctt gtcagacagg
      661 gtggcgagca aagttgactg atcgccacta aggttttaaa ctcagtcccg gcagatacga
      721 caggcgcggc agaccggcct gacgacggaa tacgaggaca aggaggagac gcgtgagcaa
      781 gatcattggc atcgacctgg gcaccaccaa cagcgtggtc gccgtgatgg agggcggtga
      841 ggcgcgggtc atccccaacg ccgaggggaa ccgcaccacg ccttccgtgg tcgccttcac
      901 gaagagcggc gagcgcctcg tcgggcaggt ggccaagcgg cagtccgtga ccaaccccaa
      961 gaacaccatc ttctcgatca agcgcttcat gggccgcaag cacgatgagg tcagcgagga
     1021 gatgaagatg gtcccctacc agatcgagcg cgccgagaac ggcgacgtcc gcgtcacggt
     1081 ggaggggaag aagtactcgc cgcccgagat ctccgcgatg atcctgggca agctcaagca
     1141 ggcggccgag gattatctcg gcgagaaggt ggagcgggcg gtcatcaccg tcccggccta
     1201 cttcaacgac tcgcagcgcc aggcgaccaa ggacgccggc cgcatcgccg gcctgacggt
     1261 ggaccgcctg gtcaacgagc cgacggccgc ggcgctggcc tacggcctcg acaagaagaa
     1321 ggaccagacc atcgccgtct acgacttcgg cggcggcacg ttcgacatct cgatcctcga
     1381 ggtgggcgag ggcgtggtcg aggtgaaggc gaccaacggc gacacccacc tgggcggtga
     1441 caacatcgac cagcggatca tcgactggct gctcgaggag ttcaagaagg accaggggat
     1501 cgacctcggc aacgacccga tggccatcca gcgcctcaag gaggcggccg agaaggcgaa
     1561 gatcgagctc tcctcggcgc aggagacgga catcaacctg ccgttcatca ccgccgacgc
     1621 ctcgggtccg aagcacatga acctgcgcct gacgcggagc aagctggagg ccctggtcga
     1681 cgacctgatc cagcgctcca tcggcccctg ccggcaggcg atcaaggacg ccggcatcga
     1741 ccccaaggag atcgacgagg tggtgctggt cggcggccag acccgcatgc cggcgatcca
     1801 gaagctggtc aaggacttct tcggcaagga gccccacaag ggcgtcaacc cggacgaggt
     1861 ggtggccgtg ggcgcggcga tccagggcgg cgtgctgtcg ggcgacgtca aggacatgct
     1921 gctcctcgac gtgaccccgc tgtcgctggg catcgagacc ctgggcggcg tgttcaccaa
     1981 gctgatcgac cgcaacacca cgatcccgac gcgcaagagc gagatcttct cgacggcggc
     2041 ggacaaccag acctcggtgg aggtccacgt cctccagggc gagcgcgaga tggcgcggga
     2101 caaccggacg ctgggcaagt tccacctggt cggcattccg ccggcgccgc gcggcatgcc
     2161 gcaggtcgag gtgaccttcg acatcgacgc caacggcatc gcccacgtct ccgccaagga
     2221 cctgggcacc ggcaaggagc agaagatcac catcacggcg tcgagcggcc tgtccgactc
     2281 cgacatcgac cggatggtgc gcgaggccga gtcgcatcgc ggcgaggacc aggagcggcg
     2341 ccgtcaggtg gaggcccgca accggctcga cggtctggtg taccagaccg agaagaccta
     2401 cgacgagcac aaggagaagc tcggcccgga cgagaagggg gagctcgaga gggcgctggc
     2461 cgaggccaag tcttcgctcg agggggagga cacgggtgcg atggagcggg cggcggagcg
     2521 gctcacccag gcctcccaca agctggccga ggcgatgtat cgccagcagc agcagggtgg
     2581 cggcgcgggt gctcagggtg ggccgggggc cggcgccggc ggtcagcagc agggctcgtc
     2641 ggacgaggtg atcgacgccg agtacgtgga cgtcgacaag agctgagtct cgagccatgg
     2701 cgccgctcaa gcagatctac ctcgaagagc tggccaggat ccaggaccgg atcaacagcc
     2761 tcttcgagca ggcgctggtg acctcggagt acgaggaccg ggaagggggt ctgccgggca
     2821 cctgggcccc ctcggtcgac gtgctggaga ccgaggactc gtacatggtc ttcgccgagt
     2881 tgcccggagt ccggcgggac gacatccaac tgcacatcca ggatcggcgg ctggagatct
     2941 ccggccgccg gcagtctcct ggagagaacc gtaatttcct ccgcatggag cgcagctacg
     3001 gtcccttccg ccggatcttc gatctggggg cgccggtcga cgccgatggg gtcaccgccg
     3061 ctttccggca gggggtcctg cgggtccaca ttccgaagcg caggggaggc gcgaccacgg
     3121 tgcccgtcag ggaggagggt gcttgatgac cggccggcgg accaagcgcg agcagaagta
     3181 cgtgatgatc agcaccgtgg ccgagcgcta cgacatccac ccgcagaccc tgcgtctgta
     3241 cgagcgg
//
LOCUS       DASPJI010000870         9680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_568838, whole genome shotgun sequence.
ACCESSION   DASPJI010000870 DASPJI010000000
VERSION     DASPJI010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9680)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568838"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1130
                     /gene="lipA"
                     /locus_tag="VEL74_24435"
     CDS             204..1130
                     /gene="lipA"
                     /locus_tag="VEL74_24435"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HYG65754.1"
                     /translation="MSETLVQIGRSTSTSVDPAAPRRQGRPEWLRIRLSTPANYHRVR
                     ALVDRLNLHTVCQEAHCPNIYECWGEHGTATFMILGDVCTRRCGFCAVTSGRPQAGVD
                     PLEPEHVAEAVSVMGLRHAVITSVDRDDLPDGGARHFAQVIDGIHRRNPETAVEVLTP
                     DFRGVPDALDVVLGARPEVFSHNMETVPRLYRQARPGSRYDRSLGLLADAARRRDAGE
                     YGGRVKTGIMVGIGETPDEVYRTLEDIRGAGVEIMTIGQYLQPTPKHLPVDRFVHPDE
                     FAAYREHALSLGFAHCEAGPLVRSSYHAHEHV"
     gene            1312..2637
                     /locus_tag="VEL74_24440"
     CDS             1312..2637
                     /locus_tag="VEL74_24440"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65755.1"
                     /translation="MLGELPPAETLEVLLHLLPGCPRCQEITSVLWAVGTEPPASGTG
                     SEGWWSASGRFAYEGTIEQVFAGVRQAYRALERERAEAPPLLEELLAERLRTREEGSL
                     WAASIPPRLRTWGLCELLLRQSQDHRADDPRRAAALAELAVVVAGGLDPSLYPPPVLE
                     TLRARTWGALGNIRRILSDFRGAEEAFGQAEIHLARGGDVVEKGVLLDLQASLRLTQG
                     RTAEATRLLDRAMLLFRRLGQRHLLGRAMISKGYVRICAGDSGPAVELLRQGLALADP
                     GHEPRLVLAAQHCLAYLLYEGGRPEEARHLLERNRRLYLEHGDRLDLVRLRYLEGKIA
                     AVLGGAGERSAAEEAFREVREVFAEAGLALDAALASLDLAALYVEQGRAAEVHVLAGE
                     MLAIFRSREVSREAMAALILFCRAAEARDVTASLVQEVTAQLRQVRRSE"
     gene            complement(2600..3610)
                     /locus_tag="VEL74_24445"
     CDS             complement(2600..3610)
                     /locus_tag="VEL74_24445"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HYG65756.1"
                     /translation="MRIVSGPEVKPQSAILCAAMSEMSEPAPPPPSPTLRVHAALLLV
                     QVIFGGFHVVAKAVLGELPPLALAGIRVALATPLLVLVAWRHDRVLPARRDIPLLVVL
                     GLLGVTANQLFFITGLEYTTAINASILMPSMPVFTAATAALLGIERIGGRRLLGIALS
                     VAGALVLVNPFRFTGGQGAALGNLLILINCLSYSLFLVFQRPLLERLPWRTLIAASFV
                     FGSLGVLPVSAPALAALDFGEVSTPAWLGVLYIVLLSTAFAYSISTWAVRRSSPALVS
                     AYSTLQPLATSVLAATLLGERFGWAEGLGLALIVAGLWQVSRPQSPARPTRSGGPAGA
                     AP"
     gene            3659..4108
                     /locus_tag="VEL74_24450"
     CDS             3659..4108
                     /locus_tag="VEL74_24450"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG65757.1"
                     /translation="MPETATDLRIRPLDELDISDIVMIDEKILGRYRPEVWERRIGYY
                     LRRDPEASVVAVADGRVVGFMLGEVRSGEFGLEEPTGWIEVLGVDPEYRGRSVGRRMA
                     EAMLEHFRGQGAQSVRTLVDEEMGDIRNFFTSLGFEAATLRPFARSL"
     gene            4142..5503
                     /locus_tag="VEL74_24455"
     CDS             4142..5503
                     /locus_tag="VEL74_24455"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HYG65758.1"
                     /translation="MSVKTTRTTTLPKNFHSAVVKWQQTYLPDYDYLMENWERYFPRE
                     PKFQLCAYREMGMCTEIECGDLKGKPKYTAAADMMAEQSHHLLGAIRAQASTEFGSIQ
                     QHRLTLARAQSDEEQFWILRMMAEELRHGYQMLHLLVEDDWSCVSKESSADMVEDILS
                     RTTGSHLLGAFNIDFDSFVDNVVFCALIDRVGKYQLAMQKVSAYQPMAESMPQMLREE
                     AFHLAAGVVPMRRWVQSAARGEGFVSMDMLQRALNKWLPRGLEMFGDERGGGTNVRYG
                     LKPMKNDEAQRQYYEEVAKLVRDLNMRYVRARLPHMERKEAEGILNRILAEGVAVEGI
                     QPEDLLYMPAPEFFRRRGVPAFTMTGFQGESFDDAEAYLDHLSRHLPDAYRVSSDYKE
                     YVDLLRQVAKGELEAKKAASRAPALRRVGGSCPCSKSVRWVIDEPRPASPHRVGAGAE
                     ANA"
     gene            5552..6454
                     /locus_tag="VEL74_24460"
     CDS             5552..6454
                     /locus_tag="VEL74_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HYG65759.1"
                     /translation="MSVFNRLLVTGLPLVPKLIVGRVASRYVAGETLDDALRVVRALN
                     GEGAMATIDVLGEEVSEREKALAAVEEYLRVFEAIQTHGLDSNVSIKLTLLGLKIDTG
                     FCRDNVERIAAAAKAHGNNFVRIDMEDHTCTDATLQIYHDLQPRYGNLGVVLQAYMRR
                     TLSDIAALPRQGGSVRLCKGIYVEPRRVAWKGYETVRANFVRALEKLIDHGVYPAIAT
                     HDEYLACQAVAIVDKHGLQRHQYEFQMLLGVDEELRRILIAGGHRLRVYVPYGGDWYP
                     YSIRRLRENPEVARHVLRAMLSGR"
     gene            6622..9030
                     /locus_tag="VEL74_24465"
     CDS             6622..9030
                     /locus_tag="VEL74_24465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HYG65760.1"
                     /translation="MSRKLPWLLLLAFAFGSLAAHAAPSALVPTPTASSYDVLDVNQI
                     GIALPAQLDLEKIGEEDMARDRKGLPPRFAVPEQVSFTPDEQGTWEQLPDGKMLWRLR
                     VVGGVGTNSLNLGFTRYKMPKGGRLLLYAADGTQVSRAFTAEDNEVHGELWTPVVLTN
                     DLIVEVTVPRRQARALELELGWINQGYRGFGTVPLAAYQKSGSCNLDVECLGSSDTWR
                     EEMRAVGVMSTGGSTFCTGSLINNTANDRKMYFITANHCGINSSNAASLVVYWNFQNS
                     FCRTPGSAASGSAGDGSLTQFHTGSIFRASYSPSDVTLVELDDPPVSSYDHHWAGWDR
                     STGNQTCTASSPCAGIHHPSTDEKRITYVTTDMVSTSYLGTASPGDGTHLWAKWATDP
                     PGPFTVPGVTEPGSSGSPLYNSAGRFVGQLHGGWSACGATGNNLSDHYGRFSVSWTGG
                     GTNSTRVSNWLDPGSTGATTTNGLNVSTGPCTPPAAPTGVSASAASASQVNLSWGAVS
                     GAVSYTVLRSTTSGGPYSSVGSATGTSFSNTGASCNTTYYYVVTASNGSCSSGNSAQV
                     QATTSACTGNVLANGVPVTGISGATGSEQFWTLSVPSGASNLQFQIAGGSGDADMYVR
                     FGSAPNTTTYDCRPYLGGNSETCTFPAPSAGTWHVMLRGYSAYSGVSLTGSYSTGSTC
                     TATAEVEPNNSTGAPQVISGACNQISGTFLNDTSTGQNDYFRLSLPAGKTVTALLNGL
                     TVDYDVYIHNSAGTQVASSTNGGATADTASWTNTGASAVNVFVRVYRYSSTRTTYQLK
                     VSYP"
     gene            9281..>9680
                     /locus_tag="VEL74_24470"
     CDS             9281..>9680
                     /locus_tag="VEL74_24470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65761.1"
                     /translation="MSRKFVWLLMLAFAGAAHAGPEALIPTPTASSYDVLDVNQVGLA
                     LPAQLDLAKIGEEDMARDRKGLPPRFAVPERVSLTPAEHGTWEELPDGKVLWRLRVVG
                     GAGTTSLNLGFTRYNMPKGGRLLLYATDGTQ"
BASE COUNT         1526 a         3447 c         3253 g         1454 t
ORIGIN      
        1 gccgcttggc caccggcgag agagaagaca gcagaagcga taaaaagtgt cctgccaaag
       61 ttcgacatac atctcccctg tgggctttta gcacgattta ttgccggcct tgacgtcttc
      121 tcgcgccctc tcacccccgg cgtctgaggt accaagcaac ggatgtgcca accccccgac
      181 gctcgacttg ttacactagc gacatgagcg agactctagt ccagatcggc cgatccacga
      241 gcacctccgt cgaccccgcc gctccccggc gtcaggggcg gccggagtgg ctgcgcatcc
      301 ggctgagcac cccggccaac taccaccgcg tccgcgccct cgtggaccgt ttgaacctcc
      361 ataccgtctg tcaggaagcc cactgcccca acatctatga atgctggggc gagcacggca
      421 ccgccacctt catgatcctg ggcgacgtct gcacccgccg ctgcggcttc tgcgccgtca
      481 cctcgggacg gccgcaggcg ggtgtcgatc cgctcgagcc cgagcacgtg gccgaggccg
      541 tgtcggtcat gggcctgcgc cacgccgtca tcacctcggt ggaccgcgac gacctgcctg
      601 acggcggcgc gcggcacttc gcgcaggtga tcgacggcat ccaccggcgc aacccggaga
      661 ccgcggtcga ggtcctgacc cccgacttcc ggggcgtgcc cgacgcgctg gacgtcgtcc
      721 tgggggcccg cccggaggtc ttctcccaca acatggagac ggtgccccgt ctctataggc
      781 aggcccgccc aggcagccgt tacgaccgca gcctcggcct cctcgccgac gccgcccgcc
      841 ggcgcgacgc cggagagtac ggcggccggg tgaagaccgg catcatggtg gggatcggcg
      901 agacgcccga cgaggtctac cggacgctgg aggacatccg cggcgccggg gtggagatca
      961 tgaccatcgg ccagtacctc cagccgacgc cgaagcacct gccggtcgac cgcttcgtcc
     1021 acccggacga gttcgcggct taccgggagc acgccctgtc cctcggcttc gcccattgcg
     1081 aggcggggcc gctggtgcgc agcagctacc acgcccacga gcacgtctga acacgagcat
     1141 gtccgagggt gtctgaaggg atggcgccgg ctggactcgc ctccggtccg gtggtactgt
     1201 gagtcaaagg ttcttcaagt gtcggtctgg cccaacttcc tgcctcgcct gtcgaaggcg
     1261 ctcctctcga aaccgaagga gcatccctcg cgcgaggatc tggagcgctt catgctcggg
     1321 gagctcccgc cggcggagac gctagaggtg ctgcttcacc ttctgcccgg ctgcccccgc
     1381 tgccaggaga tcacctccgt gctctgggcg gtgggcaccg agccccccgc ttcgggcacc
     1441 ggctctgagg gttggtggtc cgcctccggt cgcttcgcct acgaagggac gatcgagcag
     1501 gtgttcgcgg gcgtgcggca ggcctaccgg gcgctggagc gcgagcgggc cgaggcgccg
     1561 ccgctgctcg aagagttgct ggcggagcgg ctccgtaccc gggaggaggg gagcctgtgg
     1621 gccgcctcca tcccgccgcg cctccggacg tggggcctct gcgagctcct cctgcggcag
     1681 agccaggacc accgggccga cgacccgcgg cgggcggcgg ccctggcgga gctcgccgtg
     1741 gtggtggccg gcggcctcga tccgtcgctc tacccgccgc cggtgctcga gaccctgcgg
     1801 gcccggacct ggggggcgct ggggaacatc cgccgcatcc tctccgattt ccgcggcgcc
     1861 gaggaggcct tcggccaggc ggagatccac ctggcgcgcg gcggcgacgt cgtcgagaag
     1921 ggggtcctcc tggacctcca ggcctcgctg cgcctcaccc aggggcgaac cgccgaggcc
     1981 acccgcctgc tggaccgcgc catgctcctc ttccggcgtc tcggccagcg gcacctgctg
     2041 ggccgggcga tgatcagcaa ggggtacgtc cggatctgtg ccggcgattc cgggccggcg
     2101 gtcgagctgc tccgccaggg gttggcgctc gccgaccccg ggcacgagcc gcggctggtg
     2161 ctcgccgccc agcactgcct cgcctacctg ctgtacgagg gggggcggcc ggaggaggcc
     2221 cgccacctgc tcgaacgcaa ccgccgcctc tacctggagc acggcgaccg cctggacctc
     2281 gtccgcctgc gctacctcga gggcaagatc gcggctgttc tcggaggcgc cggggagagg
     2341 agcgccgcgg aggaggcctt ccgggaggtg agagaggtgt tcgccgaggc gggcctggcg
     2401 ctggacgcgg cgctggcgtc gctcgacctg gccgccctct acgtcgagca ggggcgcgcg
     2461 gccgaggtcc acgtcctcgc tggtgagatg cttgccatct tccggtcgcg cgaggtctcc
     2521 cgggaggcca tggccgccct gatcctgttc tgccgcgccg ccgaggcccg ggacgtcacc
     2581 gcctccctgg tccaggaagt cacggcgcag ctccggcagg tccgccgctc cgagtaggac
     2641 gagccgggga ttgcggccgg ctcacctgcc agagccccgc cacgatcagc gccagcccca
     2701 acccctccgc ccagccgaac cgctcgccca ggagcgtcgc cgccagcacc gacgtggcca
     2761 gcggctggag cgtgctgtag gccgacacca gcgccggcga ggagcggcgg accgcccagg
     2821 tgctgatgga gtaggcgaag gcggtggaga gtaggacgat gtagagcacc cccagccagg
     2881 cgggcgtgga gacttccccg aagtcgaggg cagccagcgc cggagcgctc accggcagca
     2941 cgccgaggct gccgaagacg aaggaggcgg cgatcagggt gcgccagggg agccgctcga
     3001 gcagcggccg ctggaagacc aggaacaggg agtagctgag gcagttgatc aggatcagca
     3061 ggttgcccag cgccgccccc tgcccgccgg tgaagcggaa cgggttgacc agcaccagcg
     3121 ccccggccac cgacagggcg atccccagca ggcgccggcc accgatgcgc tcgatgccga
     3181 gcagggccgc ggtcgccgcc gtgaacaccg gcatggacgg catcaggatc gaagcgttga
     3241 tggcggtggt gtactcgagc ccggtgatga agaagagctg gttggccgtc acccccagga
     3301 gccccaggac caccagcagg gggatgtcgc ggcgcgccgg caggacgcgg tcgtgccgcc
     3361 atgccaccag cacgaggagc ggcgtggcca gggcgacgcg gatgccggcc agggcgagcg
     3421 gcgggagctc ccccaggacc gccttggcca ccacgtggaa gccgccgaag atcacctgca
     3481 ccaggaggag agcggcgtgc acccgcaggg tgggggatgg gggagggggg gccggctcgc
     3541 tcatctcgga catggcggcg cagaggatag cggattgcgg cttcacctcc gggccggata
     3601 caatccgcat tgccggggcc ttcgccgtcc agcccggctt tctccctgga agacccgaat
     3661 gcccgagacc gccaccgacc tccgcatccg tccgctcgac gagctggaca tcagcgacat
     3721 cgtcatgatc gacgagaaga tcctcggccg ctatcgcccg gaagtatggg agcgccggat
     3781 cggttattac ctacggcggg acccggaggc ctcggtcgtg gccgtggcgg acgggcgggt
     3841 cgtcggcttc atgctcgggg aggttcgctc cggcgaattc gggctcgaag agcccaccgg
     3901 gtggatcgag gtgctgggcg tggatcccga gtaccgcggc cgttccgtcg gccgcaggat
     3961 ggccgaggcc atgctggagc acttccgcgg ccagggcgcg cagagcgtcc gtaccttggt
     4021 cgacgaggag atgggggaca ttcgcaactt tttcacctct ctgggcttcg aggcggcgac
     4081 cttgcggccg ttcgcccgat cgctgtagat ccggagagga aagcgggagc tggagccgaa
     4141 gatgagcgtc aagactacgc ggactacgac cctgccgaag aacttccact ctgccgtggt
     4201 caagtggcag cagacctatc tcccggatta tgactatctg atggagaatt gggagaggta
     4261 cttcccccgg gagccgaagt tccagctctg cgcctaccgc gagatgggga tgtgcaccga
     4321 gatcgagtgc ggcgacctca agggcaagcc caagtacacc gccgccgccg acatgatggc
     4381 cgagcagtcg catcacctgc tcggcgccat ccgtgcccag gcctccaccg agttcggctc
     4441 catccagcag caccgcctca ccctggcccg cgcccagagc gacgaggagc agttctggat
     4501 cctccgcatg atggccgagg agctgcgcca cggttaccag atgctccacc tcctggtgga
     4561 ggacgactgg agctgcgtct ccaaggagtc gagcgccgac atggtggagg acatcctgtc
     4621 gcgcaccacc ggctcccatc tcctgggcgc cttcaacatc gacttcgact ccttcgtcga
     4681 caacgtcgtc ttctgcgccc tcatcgaccg ggtcggcaag taccagctcg ccatgcagaa
     4741 ggtgagcgcc taccagccga tggccgagag catgccgcag atgctgcgcg aggaggcttt
     4801 ccacctggct gccggcgtgg tgccgatgcg ccgctgggtg cagagcgccg cccgcggcga
     4861 gggcttcgtc tccatggaca tgttgcagcg ggcgctcaac aagtggctcc cccgcggcct
     4921 cgagatgttc ggcgacgagc gcggcggcgg caccaacgtc cgctacggct tgaagccgat
     4981 gaagaacgac gaggcgcagc gccagtacta cgaggaggtc gcgaagctgg tgcgcgatct
     5041 caacatgcgg tacgtgcggg cgcgcctgcc gcacatggag cgcaaggagg cggagggtat
     5101 cctgaaccgc atcctggccg agggcgtggc ggtcgagggg atccagcccg aggacctgct
     5161 gtacatgccg gccccggagt tcttccggcg ccgcggcgtg ccggccttca ccatgaccgg
     5221 cttccagggc gagtccttcg acgacgccga ggcctacctg gaccacctgt cgcgccatct
     5281 gcccgacgcc taccgggtca gcagcgacta caaggagtac gtggatctgc tccgccaggt
     5341 ggccaagggg gagctggagg ccaagaaggc ggcctcgcgg gctcccgcgc tgcggcgcgt
     5401 cggcggctcc tgcccgtgct cgaagtcggt gcgctgggtg atcgacgaac cccgtccggc
     5461 ttccccccat cgggtggggg ccggcgccga agccaacgcc tgatcgagaa accatccctg
     5521 tgaaccagcc tgctctggag cgcggcgccc tatgagtgtg ttcaaccgcc tgctggtcac
     5581 ggggctcccc ctggttccca agctcatcgt cggccgcgtg gcgtcgcgct acgtggccgg
     5641 cgagaccctc gacgatgccc tgcgggtggt gcgcgccttg aatggtgagg gcgccatggc
     5701 caccatcgac gtgctgggcg aggaggtgag cgagcgggag aaggccctcg ccgccgtcga
     5761 ggagtacctg cgcgtcttcg aggcgatcca gacgcacggc ctcgactcca acgtgtccat
     5821 caagctcacc ctcctggggc tcaagatcga caccggcttc tgccgcgaca acgtcgagcg
     5881 catcgcggcc gccgccaagg cgcacggcaa caacttcgtg cgcattgaca tggaggacca
     5941 cacctgcacc gacgcgacgc tgcagatcta ccacgacctg cagccgcgtt acggcaacct
     6001 cggggtggtc ctccaggcct acatgcgccg caccctctcc gacatcgccg ctctgccgcg
     6061 gcaggggggg agcgtccgcc tctgcaaggg gatctacgtc gagccccgcc gggtcgcctg
     6121 gaaggggtac gagacggtac gggccaactt cgtgcgggcg ctcgagaagc tcatcgacca
     6181 cggcgtctat ccggccatcg ccacgcatga cgagtacctg gcctgccagg ccgtggccat
     6241 cgtcgacaag cacggcctcc agcgccatca gtacgagttc cagatgctcc tcggcgtcga
     6301 cgaggagctg cgccgcatcc tgatcgccgg cggtcaccgg ctgcgcgtct acgtccccta
     6361 cggcggcgac tggtatccct actcgatccg ccgcctgcgc gagaaccccg aggtggcccg
     6421 ccacgtcctg cgggccatgc tgtccgggcg ctgaagcctc tctcctcctg gaagctctgg
     6481 tggcgcgaaa atcgcgccac cccgcaccta actctgtgaa cgggtggtgt gtgcctgcga
     6541 cctgctccct gagacccccg gtccggcgtc cggcagctac gtcagtctct gacgttttcg
     6601 cttcgacagg aggagaaccc gatgagccgt aaactcccat ggctgctgct gctggctttc
     6661 gccttcggca gcctggccgc ccacgccgcc ccctctgctc tcgtgccgac ccccaccgcg
     6721 tccagctatg acgtcctcga cgtcaaccag attggcatcg ccctgcctgc acagctggac
     6781 ctggagaaga tcggcgaaga agacatggcg cgcgaccgca aggggttgcc gccgcgtttc
     6841 gctgttccgg agcaggtctc tttcacgcca gacgagcagg gcacctggga acagctccct
     6901 gacgggaaga tgctgtggcg gctgcgcgtc gtcggcggcg tcggcacgaa ctcgctgaac
     6961 ctcggtttca cccgctacaa gatgcccaag ggggggcgcc tgctgctgta tgccgcggac
     7021 ggcacccagg tcagccgcgc cttcaccgcc gaagacaatg aggtgcacgg cgagctgtgg
     7081 acgccggtgg tcctcaccaa cgacctcatc gttgaggtga ccgtgccgag gcggcaggcg
     7141 cgggcgctcg agctggagct cggctggatc aaccagggct accgcggctt cggcacggtg
     7201 cccctcgcgg cgtaccagaa gtccggaagc tgcaacctgg acgtcgagtg cctcgggtcc
     7261 agcgacacct ggcgcgagga gatgcgggcg gtgggtgtca tgtccaccgg cggctccacc
     7321 ttctgcaccg gctcgctgat caacaacacg gccaacgacc gcaagatgta cttcatcacg
     7381 gccaaccact gcggcatcaa ctcgtcgaac gcggcgtcgc tggtggtcta ttggaacttt
     7441 cagaactcct tctgccggac gccgggcagc gccgccagcg gctccgcggg tgacggctcg
     7501 ctgacccagt tccacaccgg ctccatcttc cgcgcctcct actctccctc ggacgtaacc
     7561 ctggtggagc tggacgatcc gccggtgtcc tcctacgacc atcactgggc gggctgggac
     7621 cgctcgaccg gtaatcagac ctgcaccgcc tcgtccccct gcgccgggat ccatcacccg
     7681 tccacggacg agaagcggat cacctacgtc accaccgaca tggtgagcac gagctacctg
     7741 ggcactgcgt cgccgggcga cggtacccat ctgtgggcca agtgggcgac cgatcctccg
     7801 ggtcccttca cggtgccggg cgtgaccgag ccgggctcgt cgggctcgcc gctctacaac
     7861 agcgccggcc gcttcgtcgg ccagctccat ggtggatggt ccgcctgcgg cgccacgggc
     7921 aacaacctgt ccgaccacta cggccgcttc agcgtttcct ggaccggcgg tggcaccaac
     7981 agcacccgcg tctccaactg gctcgacccg ggcagcaccg gcgccacgac caccaacggc
     8041 ctgaatgtga gcaccggccc ctgcacgccg cccgcggcgc ccacgggcgt ctcggcgtcg
     8101 gccgcctcgg cctcccaggt caacctctcc tggggcgcgg tctccggcgc cgtctcgtac
     8161 acggtcctgc gctcgaccac cagcggcggg ccctactcgt cggtgggatc ggcgaccggc
     8221 acctccttct cgaacaccgg cgcctcctgc aacaccacct actactatgt ggtgacggcg
     8281 tcgaacggct cctgcagctc cggcaactcg gcgcaggtgc aggcgacgac ctctgcgtgc
     8341 acgggcaacg tcctggccaa cggcgtgccg gtgaccggta tctcgggggc gaccggcagc
     8401 gagcagttct ggaccctgag cgtgccctcg ggcgcctcca acctgcagtt ccagatcgcg
     8461 ggcggcagcg gtgacgccga tatgtatgtg cgcttcggct cggcacccaa caccaccacc
     8521 tacgactgcc ggccctacct gggcggcaac agtgagacct gcaccttccc ggcgccgtcg
     8581 gcgggcacct ggcacgtgat gctgcggggc tactcggcct actcgggcgt gtcgctcacc
     8641 ggcagctact ccacgggctc gacctgcacc gccaccgccg aggtggagcc gaacaacagc
     8701 accggtgcgc cgcaggtgat ctccggggcc tgcaaccaga tctcgggcac cttcctcaac
     8761 gacacctcca ccggtcagaa cgactacttc cggctcagcc tgccggcggg gaagacggtg
     8821 acggccctgc tcaacggcct gacggtcgac tacgacgtct acatccacaa ctcggccggc
     8881 acgcaggtag cgagctcgac caacggcggc gcgaccgcgg acacggcgtc ctggaccaac
     8941 acgggcgcct cggcggtcaa cgtcttcgtc cgcgtctatc gctactcgtc gacccggacc
     9001 acctatcagt tgaaggtcag ctacccctga cctgacctct ccggcgcccg ccttccggtc
     9061 tctccggggg gcgggcgccc tccgcggctc gcctccggcg cggaatttcc gtcggcttgc
     9121 acagatacaa gtgaacctgg tgagaacctg gatggggaag cccgaggccc cgccttccgg
     9181 acctggcttc agccaagctc gcatcggctc taccgaggcc ctatgcgcgt cggtgatcgg
     9241 cgcgcttttt gtcgttcgaa tgacaggagg aatcaaggag atgagccgta aattcgtctg
     9301 gctgttgatg ttggccttcg ccggcgcggc ccacgcgggc cccgaggccc tgatccccac
     9361 gcccaccgcg tcgagctacg acgtcctcga cgtcaaccag gtcggcctcg ccctgccggc
     9421 gcagctggac ctggcgaaga tcggcgagga agacatggcg cgcgaccgga aggggctgcc
     9481 gccgcgcttc gccgttccgg agcgggtctc cttgactccc gccgagcacg gcacctggga
     9541 agagctcccc gacgggaagg tgctctggcg gctgcgcgtc gtcgggggcg ccgggacgac
     9601 gtccctgaac ctcggcttca cccggtacaa tatgccgaag ggggggcgcc tgctgctgta
     9661 cgcgacggac ggcacccaga
//
LOCUS       DASPJI010000871         5083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_684759, whole genome shotgun sequence.
ACCESSION   DASPJI010000871 DASPJI010000000
VERSION     DASPJI010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684759"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..1487
                     /locus_tag="VEL74_24475"
     CDS             177..1487
                     /locus_tag="VEL74_24475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HYG65762.1"
                     /translation="MSGAVTRERASAAGASGRPSFPRQLAQYIALTLRQAHEDSVLLT
                     ASALAFVTVLSLIPLLAAFSYVGTRVFHQYNQRSMEIFVQVLPYSDETVVDKLHEFLE
                     QAETLHGVGLLAFVATTLLAFATVEETMNRIWKVTRRRPLKVRMLSFAMLLFWAPLLI
                     GATFSSLILLRQSPALRRLFQESFLLNVLPLALIVLGLTLLYWRVPYTTVRFRSALAG
                     GLLAAILLEMLRQGFAAYVEFFRNVNAVYGSFAFALLFMLSIELTWAIVLLGSEAAYT
                     AQSFGVLSRIDREHPPVQAGWVGLAALYLVTERFLRGAPVLTPEALAGRLHLPQHELE
                     RALHPLLNHDLLRRTRHGSYVLAADPRQIPVEKVFEAYDHRARRGVAPLDGSARIKLE
                     ELIGTVAEGRGERLEGLTLAELVLGLAAPRRDEYNTPSPASVGQ"
     gene            1478..1553
                     /locus_tag="VEL74_24480"
     tRNA            1478..1553
                     /locus_tag="VEL74_24480"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1511..1513,aa:Ala,seq:cgc)
     gene            1679..2791
                     /locus_tag="VEL74_24485"
     CDS             1679..2791
                     /locus_tag="VEL74_24485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidinotransferase"
                     /protein_id="HYG65763.1"
                     /translation="MLKQSPAFSEVPSTAQVAVSSVVSSYNEWDPLEEVVVGVIDGAT
                     VPIWDAALRATMPGHQEAFFRGSGGRPFPAERIEAARRDLEEFVHILEAEGVTVRRPE
                     RLDHTKPFATPEWSSPGGLYAAMPRDLLLVVGDEIIEAPMAWRCRYFEVNAYRPLLKE
                     YFQRGARWTAAPRPQLNDELYPSGWEDLPGEFNSVISEFEPTFDAADFMRCGRDLFVQ
                     RSHVTNAFGIEWMRRHLGETYRIHELDIDDTHPMHVDATFVPLSPGKLLINRERIRSL
                     PSMFRTWDVMEAPEPTVPAGCTLYMSSAWLSMNILMLDEERVMVERHEETLARQLRDW
                     GFKPIRCPFSNFYAFGGSFHCATLDVRRRGELRSYF"
     gene            2800..4122
                     /locus_tag="VEL74_24490"
     CDS             2800..4122
                     /locus_tag="VEL74_24490"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HYG65764.1"
                     /translation="MQDSWKPLLEGDMADRARTALEDIARAVPAATLYQPGDFPEPTL
                     VAWRATLASGSAGKALLYAYLAFDQEARGTDGLRLDGDPGEIALDLLDRSSEAVAEVP
                     MTDSLYSGFPGIAWVNDHLAGRLFAAGDDAEAANAEVDEALLSVLERDPWPGEYDLIN
                     GLVGLGIYALEGLPRPTAARCLERVVERLSGLAERSAEGATWFSPVQSIPVYQREDYP
                     EGLYNLGASHGVAGIVGVLGAACGAGVATGSARPLLDETVRWLLAHRQGPESLCSFSH
                     FFYPGMEPGLSRLAWCYGDLGVAATLLVAARGTGEAAWERTALDIAAHAAERPDDLGR
                     VMDAGLCHGAAGVAHLFNRMARMTGDERLVRAARHWYGRALEFHQPGEGVGGFRSWSS
                     GADGQQDWREDPGFLEGAAGIGLALLGAISPVDPEWDRVLMVSARPRP"
     gene            4237..4449
                     /locus_tag="VEL74_24495"
     CDS             4237..4449
                     /locus_tag="VEL74_24495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor"
                     /protein_id="HYG65765.1"
                     /translation="MKKLFVAMALAGATVLGALSGVDTVQAGNCGPRICPELYAPVVC
                     SNGKTYPNQCYANRACATGCVSTGVF"
     gene            4653..>5083
                     /locus_tag="VEL74_24500"
     CDS             4653..>5083
                     /locus_tag="VEL74_24500"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-debranching protein"
                     /protein_id="HYG65766.1"
                     /translation="MNRGSRTLTLTLALALLALLAWTPAPASAQTQLGATLVTGGVKF
                     AVFSQNATRIEVWIFSSPPASTPTARHALTKTDTVNHIWTVTVSGLSAGTLYGYRAWG
                     PNWPYNASWTAGSNVGFSSHADSNGHRFNPNKLLTDPYAKAV"
BASE COUNT          714 a         1886 c         1654 g          829 t
ORIGIN      
        1 gctgccggcg ccgccgcagc gcatcttcct caaccatggc gaggacgcgc cgcgcaaggc
       61 gctgtcggtc gccatcgccg ccctgggctg gccgcgcccg gagctgccgc tgtcgggaca
      121 gacggtgccc tggtaggggg ccgctcccgg tcccgccctt gcatgggacc tcagatatga
      181 gcggagccgt cacacgggaa agggcatcgg ccgccggcgc gtccggccgg ccgtcgttcc
      241 cgcggcagct cgcgcaatac atcgcgctga cgctccgcca ggcgcacgag gattcggtgc
      301 tgctcaccgc ctcggccctg gcgttcgtga ccgtcctctc tctgatcccg ctcctcgccg
      361 ccttctccta cgtcggcacg cgggtcttcc accaatacaa ccagcgcagc atggagatct
      421 tcgtccaggt cctcccctac tcggacgaga ccgtggtgga caagctccac gagttcctgg
      481 agcaggccga gaccctgcac ggcgtcggcc tgctcgcctt cgtcgccacc acgctcctcg
      541 ccttcgccac ggtcgaggag acgatgaacc ggatctggaa ggtcacccgg cggcggccgc
      601 tcaaggtgcg gatgctctcg ttcgccatgc tgctcttctg ggcgccgctg ctcatcggcg
      661 ccaccttctc gagcctgatc ctgctgcgcc agagcccggc gctgcggcgc ctgttccagg
      721 agtcgttcct gctcaacgtg ctccccctgg cgctgatcgt cctggggctc acgctgctct
      781 actggcgggt cccctacacg acggtccgct tccgcagcgc cctggccggc ggcctgctcg
      841 ccgccatcct gctggagatg ctgcgccagg gcttcgccgc atacgtggag ttctttcgca
      901 acgtcaacgc ggtctacggc agcttcgcct tcgccctgct gttcatgctg tcgatcgagc
      961 tgacctgggc catcgtgctt ctggggagcg aggcggccta caccgcccag tcgttcggcg
     1021 tcctgtcgcg gatcgaccgg gagcacccgc cggtgcaggc gggctgggtc gggctcgccg
     1081 ccctctacct ggtgaccgag cgcttcctgc gcggcgcgcc ggtcctcacc ccggaggcgc
     1141 tggcgggtcg ccttcacctc ccccagcacg agctggaacg ggctctccac ccgttgctga
     1201 atcacgacct gctgcgccgc acccggcacg gcagctacgt cctcgccgcc gacccgcgcc
     1261 agatcccggt ggagaaggtc ttcgaggcct acgaccaccg ggcacggagg ggcgtggcgc
     1321 cgctggacgg cagcgcccgg atcaagctgg aagagctgat cggcaccgtt gcggagggcc
     1381 gcggcgagcg tctcgagggg ctcactttgg ccgagttggt cctcggcctt gccgcgccac
     1441 ggcgagatga gtacaatacg ccgtctcccg cctccgtggg gcagtagctc agctgggaga
     1501 gcaccacgtt cgcaacgtgg gggtcgaggg ttcgaatccc ttctgctcca ccaaattcgc
     1561 ttttcggaca acctcgagca cgatctggct caagcgggag gctctcgctg tccccctgaa
     1621 gccctcgcgc ctcaccctct gtttgcgttg gggtcctcag accagagagg aaggcgccat
     1681 gttgaagcaa tctccggcat tctctgaggt gccgtccacc gcgcaggtgg ccgtcagctc
     1741 tgttgtcagc tcctataacg aatgggatcc cctcgaggag gtggtggtcg gggtgatcga
     1801 cggcgccacg gtgccgatct gggacgccgc cctccgggcc accatgcccg gtcaccagga
     1861 ggccttcttc cgtggctccg gcggccgccc cttcccggcg gagcggatcg aggcggcccg
     1921 gcgggacctg gaggagttcg tccatatcct cgaggccgaa ggagtgacgg tccgccggcc
     1981 cgagcggctg gaccatacca agccgttcgc cacccccgag tggtccagcc ccggtggcct
     2041 ctacgccgcc atgccccgcg acctgctgct ggtggtgggc gacgaaatca tcgaggcccc
     2101 catggcctgg cgttgccgct acttcgaggt gaacgcctac cggccgctcc tcaaggagta
     2161 cttccagcgc ggcgcgcgct ggaccgcggc ccccaggccg cagctcaacg acgagctcta
     2221 cccttcgggc tgggaggacc tgcccggcga gttcaactcc gtcatcagcg agttcgagcc
     2281 caccttcgac gccgccgatt tcatgcgctg cggccgtgac ctcttcgtcc agcgcagcca
     2341 tgtgaccaac gccttcggca tcgagtggat gcgccgtcac ctcggggaga cctaccgcat
     2401 ccacgagctg gacatcgacg acacccatcc gatgcacgtc gacgccacct tcgtccccct
     2461 ctcccctggt aagctcctga tcaaccggga gcggatccgc agcctgccct cgatgttccg
     2521 gacctgggac gtcatggagg cgcccgagcc gacggtcccg gcaggttgca cgctctatat
     2581 gagcagcgcc tggctgagca tgaacatcct gatgctggac gaggagcggg tgatggtcga
     2641 acggcacgag gagacgctgg cgcggcagct ccgcgactgg ggcttcaagc cgatccgctg
     2701 ccccttctcg aacttctatg ccttcggcgg ctcgttccac tgtgccaccc tggacgtcag
     2761 gcgccgggga gagctgcggt cctacttcta ggagcgacga tgcaggattc gtggaagcct
     2821 ctactcgagg gagacatggc ggaccgggcg cgcaccgcgc tcgaagacat cgcccgcgcc
     2881 gtgcccgccg cgacccttta tcaacccggc gacttccccg agcccacgct ggtggcctgg
     2941 cgggcgacgc tggccagcgg cagcgccggc aaggcgctcc tctatgccta tctggccttc
     3001 gatcaggagg cgcgcgggac ggacggcctc cgtctcgacg gcgatcccgg cgaaatcgcg
     3061 ctcgacctgc tggaccggtc gtccgaagcg gtggccgagg tgccgatgac cgacagcctg
     3121 tacagcggct tccccggcat cgcctgggtc aacgaccact tggcgggccg gctgttcgcc
     3181 gccggagacg atgccgaagc ggccaacgcc gaggtggacg aggcgctgct ctcggtgctg
     3241 gagcgcgacc cctggcccgg ggagtacgac ctcatcaacg gtctcgtggg gctgggcatc
     3301 tacgccctcg agggattgcc ccggcccacg gcggcccgct gcctggaacg ggtggtggag
     3361 aggctctccg gcctggctga gcggagcgcc gaaggggcca cctggttcag ccccgtccag
     3421 tcgatcccgg tctatcaacg cgaggactac cccgagggtc tctacaacct gggagcctcc
     3481 cacggcgtcg ccggcatcgt cggcgtgctg ggcgccgcct gtggcgccgg ggtggccacc
     3541 ggctcggccc ggccgctcct cgacgagacc gtgcgctggc tcctcgcgca ccgccagggg
     3601 ccggagagcc tgtgcagctt ctcccacttc ttctatcctg gcatggagcc cgggctctcc
     3661 cggctcgcct ggtgctacgg cgacctgggc gtggccgcga ccctgctggt tgccgcccgg
     3721 ggtaccggcg aggcggcctg ggagcggacg gccttggaca tcgccgccca tgccgccgaa
     3781 cgtccggacg acctcggccg cgtcatggat gcgggtctct gccatggcgc cgccggcgtc
     3841 gcccacctct tcaaccggat ggctcggatg accggcgacg agcggctcgt ccgggcggcg
     3901 cggcactggt acgggcgcgc cctggaattc caccagcccg gcgaaggggt gggcggcttc
     3961 cgctcctggt ccagcggcgc cgacggccag caggactggc gcgaggaccc cgggttcctc
     4021 gaaggggcgg cggggatagg cctcgccctg ctgggagcga tctccccggt cgatcccgag
     4081 tgggaccggg tgctcatggt ctccgcccgc ccgcgtccct gagggaccgg ccctgaagga
     4141 ctggcttcag gggctgggct cgcccgcacc tgccagcaac acccttggaa gtacaaccgg
     4201 gccaccgggc cccaggattg attggaggtc tgttccatga agaagctctt cgtcgccatg
     4261 gcgctcgccg gcgccaccgt cctcggcgcc ctctccggcg tcgacaccgt ccaggccggc
     4321 aactgcggcc cgcggatctg cccggagctc tacgctccgg tcgtctgcag caatggcaag
     4381 acgtacccca accagtgcta cgcgaaccgg gcctgcgcga ccggctgcgt ctccaccggc
     4441 gtcttctagc gccaagccgg ggcgccggcc agacggcgcc ctgctcttcg ctcccctgac
     4501 gcctccccac cccgtggggc ggcaggcggg cgcggcccct gcagtacaat cacaatggct
     4561 ggatgtgcat ctctccttcc cggggagaga gggggaggcc tccatctgtt tcactcacgc
     4621 cgtgtcttcc caatcccaat gaggagatcg tcatgaatcg cggttcccgc accctcaccc
     4681 tcaccctggc tctggccctc ctggcgctgc tcgcctggac gccggcgccc gcctccgccc
     4741 agacgcagct cggcgccacc ctcgtcaccg gcggcgtgaa gttcgcggtc ttttcgcaga
     4801 acgccacccg gatagaggtc tggatcttct ccagcccccc cgcctcgacg cccaccgccc
     4861 gccacgccct gaccaagacg gacacggtga atcacatctg gaccgtgacc gtctccgggc
     4921 tcagcgccgg caccctctac ggctaccgtg cctggggccc gaactggccc tacaacgcga
     4981 gctggaccgc cggcagcaac gtgggcttca gctcccacgc cgacagcaac ggccaccgct
     5041 tcaaccccaa caagctgctg accgatccgt acgccaaggc ggt
//
LOCUS       DASPJI010000872         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_279165, whole genome shotgun sequence.
ACCESSION   DASPJI010000872 DASPJI010000000
VERSION     DASPJI010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279165"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(436..873)
                     /gene="dtd"
                     /locus_tag="VEL74_24505"
     CDS             complement(436..873)
                     /gene="dtd"
                     /locus_tag="VEL74_24505"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014464963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HYG65767.1"
                     /translation="MRIVVQRVTSASVTVDGETVGEIGRGLLLLVGIERGDGPEKVRT
                     AAGKLAGLRIFEDEAGKMNLDAAAAGGAFLVVSQFTLLGNIEGGRRPSFINAAPPEEA
                     APLVDALAEDLRQRGFKVETGRFRATMQVALVNDGPVTLVADF"
     gene            986..1426
                     /locus_tag="VEL74_24510"
     CDS             986..1426
                     /locus_tag="VEL74_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HYG65768.1"
                     /translation="MINKVILVGNLGRDPEVRSTPSGQPVATFTLATSRRWKDKNGQR
                     QEQTEWHTVVCWGRQAEVAGQYLTKGKQVYVEGRLQTRSWDDKNSGEKRYKTEVVCEN
                     FQMLGGRGGDFEGGGMGAPSHGGGGPVYDEGGYGGGPEDDDIPF"
     gene            1498..1713
                     /locus_tag="VEL74_24515"
     CDS             1498..1713
                     /locus_tag="VEL74_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HYG65769.1"
                     /translation="MRYTVILEKGREGGYVALCPALPGCVSQGLTRNEAMDNIREAAE
                     VYLEALQEDDLPIPEETGREILDIEVA"
     gene            1710..1934
                     /locus_tag="VEL74_24520"
     CDS             1710..1934
                     /locus_tag="VEL74_24520"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HYG65770.1"
                     /translation="MSRLPRGLSGRRVRRALERAGFYLKRQKGSHMVLRRDHPFAQVI
                     VPAHDQIDTGTLASILDAAGVSVDQFIELL"
     gene            2055..>2495
                     /locus_tag="VEL74_24525"
     CDS             2055..>2495
                     /locus_tag="VEL74_24525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65771.1"
                     /translation="MADREQTPPSPPLWHRLWRSPEPEISAAAASGELQVAAVRLLLL
                     SVLLYLPLREYLPAIAYTLVDGSWSVNPMRDPRLVLAVAGGSLFAALMIYSTVQRNRG
                     SSWIGFVSSALDVTLVSGMLLLVLLLGRPEEALDDAVVFPVYFLA"
BASE COUNT          424 a          828 c          825 g          418 t
ORIGIN      
        1 tgtggcaaac ggacggtgtc gtcctcgagg ccccgtctca agatctcctt ggcgatgcgc
       61 tgcttgtcgg gggatcgagg atcgaagagg tagacgagga cgttcgtatc aatcagagcg
      121 gccacgggta tagagatcct cgcgggtcca gccccgatca ccggtgcgag ggagcggact
      181 acgcctggcc tcccgctgct cctgtcgccg agttgcctca tcgaagagcc ggagccgcgt
      241 ctctcgatcg aggggtgtct gccggacggg tgggaggatg cggatggaat ctccggaagc
      301 cagccactcg acctcatccc ccggacggat gccgaactga tcggcgatct ctctgggcag
      361 ggttaccttg tactcggggc tcaccttggc catggcgtct cccatgatag caacaagagt
      421 acgtcccgga gcgcctcaaa aatccgccac cagcgtcacc ggaccatcgt tcaccagggc
      481 cacctgcatg gtggcccgga agcggccggt ctcgaccttg aagccgcgct gtcggagatc
      541 ctcggccagg gcatccacga ggggggcggc ctcctcgggc ggggcggcgt tgatgaagga
      601 ggggcggcgg cctccttcta tattgccgag gagggtgaac tgggagacca ccaggaaggc
      661 gccccccgcc gccgccgcgt ccaggttcat cttgcccgcc tcgtcctcga agatgcggag
      721 gccggcgagc ttgccggcgg cagtgcggac cttctccggc ccgtcccccc gctcgatgcc
      781 gaccagcagc aggagaccgc ggccgatctc ccccaccgtc tcgccatcca ctgtcaccga
      841 cgccgacgtc acccgctgta cgacgatccg cacctttgcc cacctccaga ggaagaaagt
      901 cgtccgaaaa gatgttccgc gcacctccgg ctccggccgg cggtgtgata ccgtagccga
      961 ttcagaccgg aggagaaagg gacgcatgat caacaaagtc atcctggttg gcaacctcgg
     1021 ccgtgacccc gaggtgcggt cgactccgtc gggccaaccg gtcgcgacct tcacgctggc
     1081 cacgagccgg cgctggaagg acaagaacgg gcagcgccag gagcagaccg agtggcacac
     1141 ggtggtctgc tggggacggc aggcggaggt ggccgggcag tatctgacca agggcaagca
     1201 ggtctacgtc gaggggcgcc tccagacccg ctcctgggac gacaagaaca gcggtgagaa
     1261 gcgctacaag accgaggtcg tctgcgagaa cttccagatg ctcggcgggc gcggcggcga
     1321 cttcgaaggc ggcggcatgg gcgccccgtc ccacggcggc ggcggccccg tctacgacga
     1381 aggcggctac ggcggcggtc ccgaggacga cgacatcccg ttttgaggag aatcggcccc
     1441 gggtctcatg ggatcggtcc cggggcctcc cctgacagat gacaggagag aggaaccatg
     1501 cgttacaccg tgatcctcga aaagggacgt gaggggggct atgtggccct gtgccctgcc
     1561 ctgccgggat gtgtctccca aggtcttacg cgcaacgaag ccatggacaa catccgcgag
     1621 gcggccgagg tctatctaga ggctctgcag gaggatgacc tcccgattcc cgaagagacg
     1681 ggtagggaga ttctcgacat cgaggtcgca tgagccgact tcctcgaggg ctctcgggga
     1741 ggcgggtgag aagagccctc gagcgcgctg gcttctactt gaagcgacag aagggaagcc
     1801 acatggtcct acggagggat catccctttg ctcaggtcat cgtccccgct cacgaccaga
     1861 tcgatacagg aacgctggcg tccattctcg atgccgctgg agtgtctgta gaccagttca
     1921 tcgagcttct ctgatccctg cccactcctg agttagactc cctcctgcga gcttcgcttc
     1981 atcagtgaat ggtctcccgt ctggcgcctc cttggaggcg gagggcgggt tgtacgtgca
     2041 ggagccttcc gggaatggca gaccgcgagc agaccccgcc ctctccgccg ctctggcacc
     2101 gcctatggcg gagcccggag ccggagatct ctgcggcggc ggcgagcggc gagctccagg
     2161 tggccgcggt ccgcctgctg ctcctctccg tcctgctcta cctgccgctc cgcgagtacc
     2221 tgcccgccat cgcctatacg ctggtcgacg gctcctggag cgtgaacccg atgcgcgacc
     2281 cgcgcttggt gctcgcggtg gccggcgggt ccctcttcgc ggcgctgatg atctactcca
     2341 ccgtccagcg caaccgcggc tcgtcatgga tcggattcgt gagcagcgcc ctggacgtca
     2401 ccctggtcag cggcatgctg ctcctcgtcc tgctcctcgg ccgccccgag gaggcgctgg
     2461 acgacgccgt ggtcttcccc gtctacttcc tggcc
//
LOCUS       DASPJI010000873         8125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_569162, whole genome shotgun sequence.
ACCESSION   DASPJI010000873 DASPJI010000000
VERSION     DASPJI010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8125)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8125
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569162"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1576
                     /locus_tag="VEL74_24530"
     CDS             32..1576
                     /locus_tag="VEL74_24530"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HYG65772.1"
                     /translation="MRLFDLVATSQRVSETRARLQKTGALADLLRRLGPEEIDIAVAY
                     LSGHLRQGRIGLGGAVVRNAISGGAGSSAAEPSLTLMEVDATFERIAGVSGAGSTAER
                     GRLLSDLLSRATHEEQDFLVRLIHGELRQGALAGLMAEALAAAAAVPAEEVRRALMLA
                     GELPAVAKAVLTEGRPGLARFHLQLYRPLQPMLAQPAGDVAEALERLGEAALELKLDG
                     ARVQVHKAEEGVRVYSRRGNDVTHAVPELVESVQALPARRLLLDGEVIALRPDGTPHP
                     FQITMRRFGRKLDVPQMRGELPLTPFFFDLLHLDGDDLIDRPAGERFAALEEAVPGLA
                     VPRRVTADPEEADAFFEEALRRGHEGIMAKATDSPYEAGKRGFSWLKVKPAHTLDLVV
                     LAAEWGHGRRKGWLSNLHLGARDPENGAFVMLGKTFKGMTDETLAWQTRKLQELALST
                     DGWTVYVRPELVVEIAFSDVQESPHYPAGMALRFARVKRYRDDKTAAEADTIDTVRKI
                     FASGRG"
     gene            complement(1597..2229)
                     /locus_tag="VEL74_24535"
     CDS             complement(1597..2229)
                     /locus_tag="VEL74_24535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65773.1"
                     /translation="MRHIHPIQPILTIALIIILVVVMRKRKAASQPAPPARGGDSRAA
                     VTRPVKPQDPPEVVFERLRQQALATLPQNLGPAGQVADEEPYGAVMEIGVPNNVVTLA
                     CFADGDAGIYYRAGGGMRGGGAHEAVRKAAKEFIALTKSAMPAMSRTTTYPLPDADMV
                     RFYALTPRGTLTVETDRQSLGNTQDPLSHLYYGGQEVVTQMRQVQEQRAS"
     gene            2440..4101
                     /locus_tag="VEL74_24540"
     CDS             2440..4101
                     /locus_tag="VEL74_24540"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HYG65774.1"
                     /translation="MRFPQTAGAILVLLLLPTFARTQEAPADPAAKVDAIFARFNTRT
                     TPGCAVAVERDGQRVLSRAYGMADLEHDVPITLDTVFEAGSVSKQFTAAAVVALARQG
                     KLSLDDDVRKYIPEVPDYGTPITLRHMMQHTSGLRDWGSVAEMEGWPRGLRAHTHAHV
                     LEIVSRQKALNFPTGSEHSYSNSGYNLMAILVDRVSGERFAEFTRKLLFEPLGMTRTQ
                     WRDDYARVVKGRAIAYGPPQDEVYRSLMPFENVHGNGGLLTTVGDLLLWNRNFEHHRA
                     GGPELVSELQHRGRLTDGREIEYAGGLYVTNYCGVPEVSHGGATGGYRAFLARYPEQR
                     LSVALLCNAAEADSGALAHQVAELYLPAGVCKEAPAGAGSSPIPLPAADLEAKAGLYR
                     NLRTGMPVRLLIQDGKLRSEQGDELLPLSRTVFESRPGRARVEFDLAPDGRPRSMRAT
                     TASGDTLPFERVADFTSTAEQLAAYPGEYRSDEAQATYTVVLEEGELVLRRRPDFSTR
                     LQPAYPDAFTIPAGLVRFIRGADGKVKEMSIGRTRVRDMRFQRVR"
     gene            4116..5057
                     /locus_tag="VEL74_24545"
     CDS             4116..5057
                     /locus_tag="VEL74_24545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65775.1"
                     /translation="MGIEHEYMIHLFDVATALEAGKLSSIKPYGPTPDGFSGGTPNQK
                     LLLAAVSYCDAVAVQAPGLSVDKAKKLVQQLIGFQKQAHCLVGKAWEPLTPSHGQLWW
                     AGWLAILALAARGKDAATEAAGVSWVRAEAALCHLSGAYQGGCYVVFTPGARGGRPDK
                     TDLTTNPARDMGYDMLVTGKMPSGSKWKNQYNVGPYFLSWLCQAQPATFQRLREPGSG
                     TFLAGPKGVEIWTEADLPNLINPLHVTRTGAEFVSWFPVLETLEPQWFAALLGSEPQF
                     GFWNGQAELKNGGPCPYPIPALSNPQYQIVVGSGGVA"
     gene            complement(5070..5768)
                     /locus_tag="VEL74_24550"
     CDS             complement(5070..5768)
                     /locus_tag="VEL74_24550"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG65776.1"
                     /translation="MIEGIRRRWREAWRGKDAGGSPPGEMERVFGEIYRSNAWGSGES
                     VSGPGSTHERAADFRDDLAAVLRDLGTRVLLDAPCGDFNWIGPVADEAERYIGVDVVP
                     ELVERNARRHGSRRRRFLRLDLTRDELPRADVVLCRDCLVHLRTADIWRALESLRRSG
                     ARYLVVTTFIGERGNEEIETGAWRPLNLERPPFHFPPPLRLIDERCLHTGGIYADKRL
                     GVWRMADVPLSPGE"
     gene            complement(5778..6617)
                     /locus_tag="VEL74_24555"
     CDS             complement(5778..6617)
                     /locus_tag="VEL74_24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HYG65777.1"
                     /translation="MKKEGSYPAEEWPTGSRWRERLHEVVFEADTQAGKTFDLALLVC
                     ILASVLAVLLESVRSLRIAYGAELRAAEWFFTGLFTVEYVLRLVAVRRPLRYARSFFG
                     LVDLAAILPTYLSLIMPGTQALLVIRALRLLRIFRILKLAHFLGEAQALKAALRASVR
                     KITVFLATVLILVVIIGALMYLVEGEVHGFTSIPQSIYWAIVTLTTVGYGDLAPVTTL
                     GKILASAVMILGYGIIAVPTGIVTVELSRRPGSVSAQACPHCGAEGHDADARFCKYCG
                     SGL"
     gene            6724..6936
                     /locus_tag="VEL74_24560"
     CDS             6724..6936
                     /locus_tag="VEL74_24560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65778.1"
                     /translation="MKRLVFSALLLLLALGLGMAPDAAAKPGCTIACESDPTITCTST
                     VGDCSYYYGAYDYIVCNGWATRCPLP"
     gene            7756..>8125
                     /locus_tag="VEL74_24565"
     CDS             7756..>8125
                     /locus_tag="VEL74_24565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65779.1"
                     /translation="MSPNPSIQDVLASLEAKVESLSRQLEVHTRQEAFHREQKEQTAA
                     ELEEVSRHLEEFRRTAGAVLQITAAERRPPAAGEDDGPNLGGRPMVSRLVAAVIAEKR
                     GDERFNASRITQEIDRRYSKR"
BASE COUNT         1221 a         2745 c         2935 g         1224 t
ORIGIN      
        1 ggcgggttcc cagccggcga agtaggagcg gatgcgcctc ttcgatctcg tcgccacctc
       61 gcagcgggtc tccgagacgc gggcccggct gcagaagacc ggagccctgg cggacctgct
      121 gcggcgctta gggccggagg agatcgacat cgccgtcgcc tacctctccg gtcacctgcg
      181 gcaggggagg atcggcctcg gcggcgccgt ggtgcgcaat gcgatatctg gaggggccgg
      241 gtcgtcggcg gcggagccct cgctcaccct gatggaggtg gacgccacgt tcgagcgcat
      301 cgccggggtg agcggcgccg gctcgacggc cgagcggggg cgcctgctgt cggacctcct
      361 ctcccgggcg acgcacgagg agcaggactt cctggtgcgg ctgatccatg gcgagctgcg
      421 gcagggggcg ctggccgggt tgatggccga ggcgctggcc gcggcggcgg cggtgccggc
      481 cgaggaggtg cgccgggcgc tgatgctggc cggcgagctg ccggcggtgg ccaaggccgt
      541 gctcaccgag gggcggccgg ggctcgcccg cttccacctc cagctctacc ggccgctcca
      601 gcccatgctc gcccagccgg cgggggacgt cgccgaggcg ctggagcggc tgggggaggc
      661 ggcgctggag ctcaagctcg acggcgcgcg ggtgcaggtg cacaaggcgg aggagggggt
      721 gcgggtctac tcgcgccggg gcaacgacgt cacccacgcg gtgccggagc tggtcgagtc
      781 ggtccaggcc ctgccggcgc ggcggctgct gctcgacggc gaggtcatcg ccctgcggcc
      841 ggacggcacg ccgcatccgt tccagatcac catgcgccgc ttcggccgca agctggacgt
      901 cccccagatg cgcggtgagc tccccctcac ccccttcttc ttcgacctcc tgcacctgga
      961 cggcgacgac ctcatcgacc ggccggcggg ggagcgcttc gcggcgctcg aggaggcggt
     1021 ccccggcctc gccgtgccgc gccgggtgac cgccgacccc gaggaggccg acgccttctt
     1081 cgaggaggcg ctgcggcgcg ggcacgaggg gatcatggcc aaggccacgg actctcccta
     1141 cgaggcgggc aagcggggct tctcgtggct caaggtgaag cccgcgcaca ccctggacct
     1201 ggtcgtcctg gccgcggagt gggggcacgg ccggcgcaag ggatggctgt cgaacctcca
     1261 cctgggcgcc cgcgatccgg agaacggcgc cttcgtcatg ctgggcaaga ccttcaaggg
     1321 gatgaccgac gagaccctcg cctggcagac ccggaagctg caggagctgg ccctctcgac
     1381 cgacggctgg accgtctacg tccgccccga gctggtggtg gagatcgcct tcagcgatgt
     1441 ccaggagagc ccgcactacc ccgcgggcat ggccctgcgc ttcgcccgcg tcaagcgcta
     1501 ccgcgacgac aagaccgccg ccgaggccga caccatcgat accgttcgga agatcttcgc
     1561 gtcggggagg ggctgagccc ctccccgact gggggatcac gacgcccgct gctcctgcac
     1621 ctggcgcatc tgggtgacga cctcctggcc gccgtagtag aggtgcgaca gcgggtcctg
     1681 ggtgttgccc agggactggc ggtcggtctc gacggtgagg gtgccgcgcg gcgtcagggc
     1741 gtagaaccgc accatgtcgg cgtcggggag agggtaggtg gtggtgcggc tcatcgcggg
     1801 catggcgctc ttcgtcaggg cgatgaactc cttggcggcc ttgcgcacgg cctcgtgggc
     1861 gccgccgccc cgcatgccgc cgcccgcccg gtagtagatg cccgcgtcgc cgtcggcgaa
     1921 gcaggccagg gtgacgacgt tgttgggcac cccgatctcc atcaccgcgc cgtagggctc
     1981 ctcgtcggcc acctggccgg ccggaccgag gttctggggc agggtcgcga gcgcctgctg
     2041 gcgcagccgc tcgaagacca cctccggcgg gtcctggggc ttgaccggcc gcgtgacggc
     2101 ggcccgggag tcgccccctc gcgccggggg agccggctgg ctcgcggcct tccgcttccg
     2161 catgacgacg acgaggatga tgatgagggc gatggtgagg atgggctgga tagggtggat
     2221 atgtcgcatc ggctcgttct ggctccgcgt tctgtccgtc tgaggcgcac ccgtacgtcc
     2281 cacccgaggc aagggatgcg gggatggccc tctctgcatt cgctctgttg gtccgatgct
     2341 acaggagaag cggcgccggt gataggggga gggctagccg ggcggctcct ctgctaatct
     2401 cctggccgga tccaaaccga ttctcgtgag gtccccgtca tgaggtttcc gcagaccgcc
     2461 ggtgccatcc tcgtcctcct gctcctcccg acgttcgcgc gtacgcagga ggctccggcc
     2521 gatcccgccg ccaaggtgga tgccatcttc gcgaggttca atacccggac gacgccgggc
     2581 tgcgccgtcg ccgtcgagag ggacggccaa agggtcctct cccgggccta cggcatggcc
     2641 gacctggagc acgacgtgcc gatcaccctc gacaccgtgt tcgaggccgg ctccgtctcg
     2701 aagcagttca ccgcggcggc cgtggtggcc ctggcgcggc agggcaagct gtcgctggac
     2761 gacgacgtcc gcaagtacat ccccgaagtg ccggactacg gcacgcccat caccctgcgc
     2821 cacatgatgc agcacacgag cggcctgcgc gactggggct ccgtggcgga gatggagggc
     2881 tggccgcgcg gactgcgggc ccacacccac gcccacgtgc tggagatcgt ctcccggcag
     2941 aaggcgctca acttcccgac cgggtccgag cactcctaca gcaactcggg ctacaacctg
     3001 atggcgatcc tggtggaccg ggtgagcggc gagcgcttcg ccgaattcac ccgcaagctc
     3061 ctcttcgagc ctctcggcat gacccgcacc cagtggcgcg acgactacgc ccgcgtggtg
     3121 aagggccggg cgatcgccta cggcccccct caggacgagg tctaccgcag cctgatgccg
     3181 ttcgagaacg tccatggcaa cggcggcctg ctgacgacgg tgggggacct gctcctctgg
     3241 aaccggaact tcgagcacca ccgggccggc gggccggagc tggtctccga gctccagcat
     3301 cgcggccggc tgaccgatgg ccgggagatc gagtacgccg gcgggctcta cgtgaccaac
     3361 tactgcggcg tgccggaggt gagccatggc ggcgcgaccg gcggctaccg ggccttcctg
     3421 gcgcgctatc ccgagcagcg gctgtcggtg gcgctcctct gcaacgcggc ggaggcggat
     3481 tcgggagccc tcgcccacca ggtggccgag ctctaccttc ccgcgggggt ctgcaaggag
     3541 gcccccgccg gtgccggcag ttcccccatc ccgctcccgg ccgcggacct cgaggccaag
     3601 gcgggcctct accgcaacct gcgcaccggc atgccggtgc ggctcctgat ccaggacggc
     3661 aagctccggt ccgagcaggg ggacgagctg ctgccgctgt cgcgcaccgt cttcgagtcc
     3721 cggccgggcc gcgcgcgggt ggagttcgat ctggcgccgg acggccgtcc caggtcgatg
     3781 cgggccacga ccgcgagcgg cgacacgctc cccttcgagc gggtggcgga cttcacgtcc
     3841 acggccgagc aactcgccgc ctacccaggg gagtaccgca gcgacgaggc gcaggccacc
     3901 tacacggtgg tgctggagga gggtgagctc gtcctgcggc gccggccgga cttctcgacc
     3961 cgcctccagc cggcctaccc ggacgccttc accatcccgg ccggcctggt gcgcttcatc
     4021 cgcggcgccg acggcaaggt gaaggagatg agcatcggca ggacccgggt gcgggacatg
     4081 cggttccagc gggtgcgtta acgaggagga atgcgatggg aattgagcat gagtacatga
     4141 tccacctgtt cgacgtggcg accgctctgg aggcgggcaa gctctcgtcg atcaagccct
     4201 atggtcccac tcccgacggt ttctcgggcg ggacgcccaa ccagaagctg ctgctggccg
     4261 cggtgtccta ctgcgacgcc gtcgcggtcc aggcgccggg cctcagcgtc gacaaggcaa
     4321 agaagctcgt ccagcaactg atcggttttc agaagcaggc gcactgtctg gtcgggaagg
     4381 cctgggagcc tctgacgccg tctcatgggc agctctggtg ggcgggatgg ctcgccatcc
     4441 tcgcgctggc ggcccggggc aaggacgccg ccaccgaggc cgccggcgtt tcctgggtgc
     4501 gcgccgaggc ggccctctgc cacctctccg gcgcgtacca gggcggttgc tatgtcgtgt
     4561 tcaccccggg cgcccgcggt ggccgacccg acaagacgga cctgaccacc aatccggccc
     4621 gcgacatggg ctacgacatg ctggtcaccg ggaagatgcc gtccggctcc aagtggaaga
     4681 atcagtacaa cgtgggcccc tacttcctct cctggttgtg ccaggcgcaa cctgccacgt
     4741 tccagcggct gcgtgagccc ggctcgggga ctttcctcgc cggccccaag ggtgtggaga
     4801 tctggaccga ggccgatctg ccgaacctga tcaatccgct ccacgtcacc cgcaccggcg
     4861 cggaattcgt ctcgtggttc cccgtgctcg agaccctgga gccgcagtgg ttcgccgcgc
     4921 tgctgggcag cgagccgcag ttcgggttct ggaacgggca ggcggagctc aagaacggcg
     4981 ggccctgccc gtatccgatc cctgcgctct cgaacccgca gtatcagatc gtcgtcggct
     5041 cgggaggcgt ggcgtagggt tctcgaatcc tattctccgg gtgacaacgg gacgtccgcc
     5101 atgcgccaga cgccgagcct cttgtccgcg tagatcccgc cggtgtggag gcagcgctcg
     5161 tcgatgagcc tcaggggcgg ggggaagtgg aaaggggggc gttcgaggtt gagcgggcgc
     5221 caggcgcccg tctcgatctc ctcgttgccg cgctcgccga tgaaggtggt caccacgaga
     5281 tagcgcgcgc cgctccgccg caggctctcc agggcgcgcc agatgtcggc ggtgcggaga
     5341 tggacgaggc agtcgcggca gaggaccacg tcggcccgcg gcagctcgtc gcgggtcaga
     5401 tcgagccgca ggaagcgccg acgccggctg ccgtgccgtc gggcgttgcg ctccaccagc
     5461 tcggggacga cgtcgacgcc gatgtagcgc tcggcctcgt ccgcgaccgg cccgatccag
     5521 ttgaagtcgc cacacggggc gtcgagcagc acgcgggtcc ccaggtcccg caggacggcg
     5581 gcgagatcgt cccggaaatc ggcggcccgc tcgtgggtcg accccggccc cgagacggac
     5641 tcgccgctgc cccaggcgtt cgagcgatag atctctccga agacacgctc catctccccc
     5701 ggagggctgc ctccggcgtc cttgcccctc caggcctcgc gccagcggcg ccggatgccc
     5761 tcgatcatcg gaggtgttca gagcccggag ccgcagtact tgcagaagcg ggcatcggca
     5821 tcgtgcccct cggcgccgca atgcgggcag gcctgggccg agacggagcc ggggcgccgg
     5881 gagagctcga cggtgacgat gcccgtcggc accgcgatga tgccgtagcc gaggatcatc
     5941 acggccgagg caaggatctt gcccagcgtc gtgaccggcg caaggtcccc gtagccgacg
     6001 gtggtcaggg tgacgatcgc ccagtagatg ctctgcggga tgctcgtgaa gccgtgcacc
     6061 tccccctcga cgaggtacat cagggcgccg atgatcacca ccaggatgag gaccgtggcc
     6121 aggaagacgg tgatcttgcg gacgctggcg cgcagggcgg ccttgagggc ctgggcctcg
     6181 ccgaggaagt gggcgagctt gaggatccgg aagatccgca gaaggcgcag cgcgcggatg
     6241 acgagcaggg cctgggtgcc cggcatgatc aggctcagat aggtggggag gatggcggcg
     6301 aggtcgacca gcccgaagaa gctgcgggca tagcggagag gccgccggac ggccaccagg
     6361 cgcaggacgt actcgacggt gaacagtccg gtgaagaacc actcggcggc gcgcagctcc
     6421 gcgccatagg cgatccgcag cgaccgcacg ctctcgagca gcaccgccag gacgctcgcc
     6481 aggatgcaca ccaggagcgc gaggtcgaac gtcttgcccg cctgggtgtc cgcctcgaag
     6541 acgacctcgt ggagccgttc ccgccagcgc gagccggtgg gccactcctc ggcggggtag
     6601 gacccttcct tcttcacccg gggatcatat cccccgcgcg gtccgtccgg gtcgccgtgg
     6661 ctcgcgcagt cggcggggag cacccatcct cttcaggggg acatctcccg gaggaggaca
     6721 tccatgaaga gattggtttt ctcggctctc ctgctgcttc tggccctcgg gctcggcatg
     6781 gcgccggacg ccgcggccaa gccgggctgt acgatcgcct gcgagtccga tcccacgatc
     6841 acctgtacga gcacggtggg agattgctcc tactactacg gcgcctacga ctacatcgtc
     6901 tgcaacggct gggcgactcg ctgcccgctg ccgtagccgc ctcatccccc gcatgacggc
     6961 gggatccgga gccggcggcg actacgccgc cggctccgcc cccgctcctg ccagagattc
     7021 gaagcccaaa ctgtcgcagc gtaccggtga attgtctcgc cgtaactttg gcttgcgaca
     7081 gtttcggacc cggcgcaccg gccggcggct gcaactgtcg cggagagctg gtcggcgaga
     7141 cgccgtaacg ttcaggtctc tcgctggacc ctgtctttgt cgcgcagacc tctaggttgt
     7201 agcagagggg ctccaggctg tcttaccaga atgttgactt gtcgcggatc attgttgaaa
     7261 gcatcgccat gagtcttgag gtgtcgctct ggaggcttgc gatgtcgccg agttgtcgta
     7321 gggtgttgta gatgactcga cgatgtcgcg gagtagctgc gaactgtctc tgtgcatcga
     7381 tgaaatgtcg tagggtgtcg cgatgcggcc gattggaaga gtgcgacgcg acaatgtaac
     7441 ggtacgctgc gacatctgca cgctacggcg ggatgatacc ccctgccgcg cgacagtggt
     7501 ggattgcggg aagccgccgg agatggtaat gtcgcggagt agtcgcaaag tgtcgcggag
     7561 gaaggttcgc gcgcccggtc ggaggggacg gcgcgacatc ggtccgttct ggcgcctgga
     7621 cccaggcttc tgcgcgacag atctgcgctt cggagaggcg ccggaacggt ccggcgtgtc
     7681 gccggcggtg gtgccgcgac aacttgaacg tccggcgcgc cgtcgaaggg gacggcgcga
     7741 cagagagggg atacgatgag cccgaatccg tccatccagg atgtcctggc cagtttggag
     7801 gcgaaggtgg agagcctgtc gcggcagttg gaggtgcata cccggcagga ggccttccat
     7861 cgggagcaga aggagcagac ggctgccgag ctggaggagg tatcgcggca cctggaggag
     7921 ttccggcgga cggcgggcgc ggtgctccag atcacggccg cggagcggcg gccacccgcg
     7981 gccggggagg acgacggccc gaacctcggc ggcaggccca tggtgagccg tctggtcgcc
     8041 gccgtcatcg ccgagaagcg aggcgacgag cgcttcaacg cctcgaggat cacccaggag
     8101 atcgaccgcc gctacagcaa gcggc
//
LOCUS       DASPJI010000874        37098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_56344, whole genome shotgun sequence.
ACCESSION   DASPJI010000874 DASPJI010000000
VERSION     DASPJI010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 37098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37098)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37098
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56344"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VEL74_24570"
     CDS             complement(<1..275)
                     /locus_tag="VEL74_24570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65780.1"
                     /translation="MDEPLYLLPQTERLLRRLESLEAPLVQVWGWPGAGKSALLRALL
                     EKQGTRSQGLSLGELSRDGKIRETLEAAHDTGVRWLIVSGRPGEALK"
     gene            complement(371..937)
                     /locus_tag="VEL74_24575"
     CDS             complement(371..937)
                     /locus_tag="VEL74_24575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG65781.1"
                     /translation="MESPEAGSSFDDFLKKVEPTLKRILVKYRVPSGDAEDLLQQALL
                     ALLYQWDRVRDPEAWLVGTLRRHCLMYWRTRRRRIYAAVDETLLEWLSQPVDPPQEKA
                     DLLSDLAGVIERLPAKCQSLFRLRFRLGYEAPEVAEKLGYRMSSIGKITTRCFAALWR
                     QVLAAGLAERASNDSEVSAGELKALLRR"
     gene            complement(1043..1813)
                     /locus_tag="VEL74_24580"
     CDS             complement(1043..1813)
                     /locus_tag="VEL74_24580"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HYG65782.1"
                     /translation="MSDLLAYLGEIGYGDVYLRPRAPRAPVATVVAPEPAAIPQEAAI
                     QEMASPEVASPEPAAPALPVAADDAPERAAALAALGASTEGCTRCRLAEGRHKVVFGS
                     GNPNADLMFIGEGPGAEEDRQGLPFVGAAGELLTRIIQAMEMTRDEVYIANVVKCRPP
                     GNRDPQPDEVAACRGYLERQIALVRPRVVVALGRIAAQALLGTDSPIGQMRGRWYTIQ
                     GVPAMVTYHPAALLRNPALKRPTWEDLQQVRDHLREGV"
     gene            complement(1806..3020)
                     /gene="coaBC"
                     /locus_tag="VEL74_24585"
     CDS             complement(1806..3020)
                     /gene="coaBC"
                     /locus_tag="VEL74_24585"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HYG65783.1"
                     /translation="MRVLLGVSGGIAAYKAAELVRRLRARGHDVRCALTRSAASFVTP
                     LTLEVLSGHAVHQEEYLEPTGSGEEAHITAAAWAEVLCVAPATAHVLGRLALGLADDF
                     LTTTALAFAGPVVAAPAMHSVMWSQPAVQEHVDTLRRRGVWFAGPVEGPLASGEVGMG
                     RMADPDAIVEAVEAVAGRGPLAGRTVLVTAGPTHEPVDPVRFLGNRSSGKMGFALAAE
                     AARRGARVVLVAGPVALATPPGVRRIDVTTAREMERAVREHAPEADLVIMSAAVADYR
                     PSRPAARKIKKTQGLAAIELEENPDILAGLVAVAPDAVRVGFAAETDDLEANARRKME
                     AKGVDFLVANDVSRPDIAFGSEANEVTVFRREGPPLFVPRSPKSAIAGQLLDLFAVLL
                     PSHERTAVRPVV"
     gene            complement(3028..3267)
                     /gene="rpoZ"
                     /locus_tag="VEL74_24590"
     CDS             complement(3028..3267)
                     /gene="rpoZ"
                     /locus_tag="VEL74_24590"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HYG65784.1"
                     /translation="MEPKTPPTVDSKFRLVLVAASRAEQLMRGARAKVEAGKRKSTTV
                     AMEEIHQKAVDWGYGPPPPKETAEAEGETAAAEVH"
     gene            complement(3324..3986)
                     /gene="gmk"
                     /locus_tag="VEL74_24595"
     CDS             complement(3324..3986)
                     /gene="gmk"
                     /locus_tag="VEL74_24595"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HYG65785.1"
                     /translation="MTSAGELFILSAPSGAGKTTLIQSLIQGLKQGSFAGLGDLAFSV
                     SYTTRAPRGAEIDGRDYHFVDHATFRGMIDAGRFLEWAEVHGNFYGTANDEVFPRLAK
                     GTDVLLDIDVQGAERVLHRHPEAHGIFVMPPSYEDLESRLRRRAQDDEQTIARRLAVS
                     LREVRRFEQYRYVIINDDARRASDVLAAIILEKRHRRDRMLPRIQAILLDFQERAPAS
                     QA"
     gene            complement(4013..4891)
                     /locus_tag="VEL74_24600"
     CDS             complement(4013..4891)
                     /locus_tag="VEL74_24600"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HYG65786.1"
                     /translation="MRSMTGYGEASAENARHIVSVSLKAVNHRFLDLALRIGDEIRSS
                     ESAVRELLGRELSRGRVEVRIDARPRAERHATVEFHLGIARAAHAATHRLVEEGLIER
                     GLGAGDLLRIPEAFRVDVEAEEWDEADHELLLKVVRDAVSQLVSGRLLEGASLAAAME
                     ERLRGLAEIVARLDELRGPVREEMAATFRRRVGELLDGRAVDEARVAQEIALLIDRSD
                     VSEEIDRLRSHLEHFRSVAAEPGPTGKRLDFLTQEVFRELNTLGAKCRNAEMTRAVLD
                     AKVLCEQIREQVQNVE"
     gene            complement(4905..5558)
                     /locus_tag="VEL74_24605"
     CDS             complement(4905..5558)
                     /locus_tag="VEL74_24605"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HYG65787.1"
                     /translation="MSRDIAILSLLGAGFIRGLAASVRRRYHGDELVRQWEQRGEHFI
                     LAFWHRHMLLMPYGYRGRRMTVLSSHSRDGEIMVRTLARLGIDTCRGSTTRGGAAGLR
                     GLLRMAAEGSDLGFTPDGPRGPAGEVQQGVIIAAAMSGFPIQPAALAATRARRLGSWD
                     RMVVPLPFSTVHYVYGEPLYVERRADPAAAAAELKRRLDAAEAEAERLAGRSGGGSE"
     gene            complement(5555..7468)
                     /locus_tag="VEL74_24610"
     CDS             complement(5555..7468)
                     /locus_tag="VEL74_24610"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HYG65788.1"
                     /translation="MSIRAFFWHYIRRYVGWAMLAALAMVVYAGTMAGLSFLIGPIFG
                     EVLQYGDQLPGLGGIVGSPGSGDSAGDAAAAGDAARPPGEPAFLDDLKKRFDVADQID
                     DSYESLKRRLGVGPDEVVFFVPLLFVLVILIRSLADFLSGYAFQHIGLGVTTDIRNDL
                     YWHILGQSSRFHAEHPSGELVARIINDVAVMQNAVSNRLLDLFQQSITLLFLLGLLLS
                     THFRLAVISLVVAPVLVYPIVRFGKGMRRTSHRSQERMADLASLMAEGIRGHRVVKAF
                     GMEEFEYGRFREATRRHLRVNLWAQMLTHASGPVVESLAAFGAAALLIYAGKSIRAGE
                     LTPTQLVQFLFTLYMLYDPIRKLNKVNLILQEASAAGQRVSRLLELPNDIEQRPGART
                     VERVEKGIAFERVSFAYEDRPALWDVDLAVRAGEIVALVGPSGAGKSTMVNLLPRFFD
                     PDTGRIALDGTDIRDLELKSLRSLIGMVTQDTVLFNDTIRNNIAYGRSDLPLERVREA
                     AAAAYADEFIMQLPQGYETVIGESGVRLSGGQRQRLAIARALLKNAPILILDEATSHL
                     DSQSEALVQKALYNLMQGRTTLVIAHRLSTVQRADRIAVMEAGRIVEEGSHEQLMALG
                     GSYKRLHDLQFRV"
     gene            complement(7465..8685)
                     /gene="lpxB"
                     /locus_tag="VEL74_24615"
     CDS             complement(7465..8685)
                     /gene="lpxB"
                     /locus_tag="VEL74_24615"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HYG65789.1"
                     /translation="MTSMVGGERSPDLRVDLLVVAGEASGDLHAARLVSELRRRVPGL
                     STFGLGGDELMAAGLDAVAHSAEISVVGITEVLKILPRARQIFAEILRQVDRRRPRAA
                     VLVDFPDFNLRLAKELRRRGVPVVYYISPQVWAWRRGRVKTIAKVVDRMLVLFPFETS
                     FYQGHGVNVVHVGHPLVDEVPRLPQVWEEGEPEGPFRLALLPGSRRSEIEALLPTMLE
                     AVRRIALELPVQVSLIQAPSVPRELLEEKIVAAGLPVERVEIIARDRFDAIAGSHLAL
                     CASGTATLEVGLLGTPMVVLYRLATGTYVLARMLVRLPHYSLVNLVLGRQVVPELLQG
                     AAQPDRIADEARRLLTETAPREEVRAGLAELRGRLGEGGASARAAREVAAFLGFSGEP
                     VDAGAREAAEGVRA"
     gene            complement(8682..8876)
                     /locus_tag="VEL74_24620"
     CDS             complement(8682..8876)
                     /locus_tag="VEL74_24620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65790.1"
                     /translation="MLSHFFLMVLYALVVSLFFTLLWRRERKEQTRLFLQLFLGMVAG
                     GLLVAWLMYPFPPGPPAPIP"
     gene            complement(8881..9294)
                     /locus_tag="VEL74_24625"
     CDS             complement(8881..9294)
                     /locus_tag="VEL74_24625"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HYG65791.1"
                     /translation="MEPGSTSAPQTTAAAGVGSALVAAVAAWLVPGLGHVILRRWQRG
                     VVFFLLVLVSIVVGCSLQGNLYRVLPNQPLTLLATLGSMGMGIPYFLLRYGMGYAGSI
                     VGPGYEYGTSFLLTAGLMNLLLVLDAWDIGRGKKD"
     gene            complement(9295..9804)
                     /locus_tag="VEL74_24630"
     CDS             complement(9295..9804)
                     /locus_tag="VEL74_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HYG65792.1"
                     /translation="METLFAPWRHAYITSHKPPDETPDAPACFFCAAAKRPDDPEGLV
                     VHTARHHLVMLNLHPYASGHLMVAPLEHLASPLDSGEEARQEFWPLVLETQRVLAAAY
                     QPHGFNLGMNLGAPAGAGVPGHFHFHLVPRWKGDTNFMTVLADVRVVPEDLRQARDRL
                     RALFARKES"
     gene            complement(9854..10135)
                     /locus_tag="VEL74_24635"
     CDS             complement(9854..10135)
                     /locus_tag="VEL74_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HYG65793.1"
                     /translation="MTKAELVEEVARTTQLTKKHAEIIVNTVFDSIVDSLKEGEKIEL
                     RGFGSFRIRRRGARTGRNPKTGEKVSVPPKRIPYFKPGKELRELLNDEC"
     gene            complement(10235..11968)
                     /locus_tag="VEL74_24640"
     CDS             complement(10235..11968)
                     /locus_tag="VEL74_24640"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HYG65794.1"
                     /translation="MTAESDYSEQDYQKLLELYDESMRNLTEGEIVPGRVIGVTSNSV
                     IVDVGYKSEGLVPIEEFTDRDGNLTVEIGEEVDVLLEKTEDLEGHVLLSRTKAQRMRR
                     WTEVERAYKESKVIKGRVTDRIKGGLTVDVGLRAFLPGSLVDIKPVKNLESLRGQELE
                     FKVISLDRRRNNIVLSRKAVLETELQRRKADTLTRLEEGAMLRGVVKNITDYGVFIDL
                     GGIDGLLHITDISWGRVSHPSEHFSVGDEVDVIVLKFDPDTERVSLGYKQRSDDPWTL
                     VDKKYPIGSRVHGRVVSLVDYGAFVEIEEGVEGLIHVSEMSWTKKVVNPAKVLEVGDE
                     VQAIVSELDMDQRRISLSLRQTERNPWEELQDSHPIGTIIEGKVRNLTEFGAFVEITE
                     GIDGLIHVSDMSWTKRVKHPNEVLKKGDTVRARITNIDVENQRVSLSIKEFMPNEWED
                     FAREHKVGDLLDGRVVNVTDFGLFIDIYNGLEGLAHVSEIEVGQGVRLEDFYSVGDWV
                     RTRVLRIEEGDKKVGLSMRGVSQPTDEEIEELNQATAGQVVGGGEEEAKAEPRAKKRA
                     VREEHPEEDEE"
     gene            complement(12187..13344)
                     /gene="queG"
                     /locus_tag="VEL74_24645"
     CDS             complement(12187..13344)
                     /gene="queG"
                     /locus_tag="VEL74_24645"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HYG65795.1"
                     /translation="MDPLDPRATAELLKAWALEAGFDRAGVATLEPLEHGPSLLRWLE
                     RGDQADMSWMERRVEARLDPGQVHPGARSVLFVALQYHPLSKEDGTEEAEPDGDLWPR
                     VARYARGKDYHEVMGARLKALAARVREAFPGCDTRPYVDTGPVLERELAARAGMGAVG
                     KNTLLLHPEGGSWFLLGELFLTLDLAPDVPLADMCGSCTRCLEACPTGALAEPYRLDS
                     RRCISYWTIEHRGSLPPEAREMVGEWVFGCDLCQEACPWNTLPEGSTHPELELPPERG
                     ELDLARLLLLGRDEYVERFRGNPMKRPKLEGLQRNAAVAMGNRGSARYVPALRQALEE
                     GTPLVQEHAAWALERIGGAGNPPPDLPHSLPPTLPGVEAPPPPAPGVRARP"
     gene            13689..13826
                     /locus_tag="VEL74_24650"
                     /pseudo
     CDS             13689..13826
                     /locus_tag="VEL74_24650"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            14091..14930
                     /locus_tag="VEL74_24655"
     CDS             14091..14930
                     /locus_tag="VEL74_24655"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYG65796.1"
                     /translation="MEGSSPVSRMVNLFREDASLVECVTWLREIYLRRLEKKPGAAEL
                     EKAVLCLLNDDLLPEKTRVDGIDSEALWVVQHGARLPLQELSDGYRTTAALVMDIVRH
                     LYLTFGELRIETATDDEGTFQRVLHEGVVLIDEVETHLHVSWQKKIGFWLKRHFPHIQ
                     FIVTTHSPFVCQAADPGGLIRLPAPGENRPVEHVPESLYYTVVNGGADDAVLTELFGL
                     DSPYSDESEKLREEIAQLEARVQQGKATEGDLQSLDQMRSRLPRTMTTDVELALKKLA
                     ADL"
     gene            14927..15649
                     /locus_tag="VEL74_24660"
     CDS             14927..15649
                     /locus_tag="VEL74_24660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HYG65797.1"
                     /translation="MRRLHRTPLSDKTRIFLRKRAGQVADALHPIAEAKRLWDRLDNK
                     AFREIRQTLAAMASGVERCMYCEDSEGTAIEHFWPKALYPKRAFDWNNYLLACSRCNS
                     NYKRNRFPLDPAGRPLLVNPVEEQPLDHLAFSPSTGRYEPRTNKGRASEEVYGLNRTK
                     LTRGRSNAWTVLQKLIVVYAQAKEEGDLAEAAKIETALREHPFAGVLAALLHLAAGPG
                     AALVHPASLQALLQNPEIQGWA"
     gene            complement(16027..16371)
                     /locus_tag="VEL74_24665"
     CDS             complement(16027..16371)
                     /locus_tag="VEL74_24665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65798.1"
                     /translation="MPSHSDRTPNGVDLSRPTESTRRALYDLVVRIHFTPTPEEIQMA
                     KEDPGNHWIPDYGPDAAELTVWYDRGRWYASWLSLEEEPDPRAPWQRWSVVRIVEDAD
                     PSSPAGVTFLEV"
     gene            complement(16699..16935)
                     /locus_tag="VEL74_24670"
     CDS             complement(16699..16935)
                     /locus_tag="VEL74_24670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65799.1"
                     /translation="MKAKLVYVCAMLCVVLLAVVVAPSNTLACSGDDCGCYLATFDCL
                     ANCPPIGHPDRLSCEVACNRAEVRCAIACCCPQC"
     gene            complement(16996..18846)
                     /gene="uvrC"
                     /locus_tag="VEL74_24675"
     CDS             complement(16996..18846)
                     /gene="uvrC"
                     /locus_tag="VEL74_24675"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HYG65800.1"
                     /translation="MAHERVVERIRELPDRPGIYIFKDAAGKVLYVGKAKSLRKRTAS
                     YLNRDLDTRLAAMIAEAADIDTVLTDSEAEALLLENNWIKDRRPRYNVLLRDDKTYPY
                     LKLTSADEYPRLAFTRRISNDKADYFGPFLPGGLARKAIKLVQKLFQVRVCRIEIDGR
                     LPRPCLYYDMHRCLGPCVAGLTTKGAYDEAVQQARLFLAGKNDQLVKRLKREMWDAAE
                     SMDYERAAQLRDALSEVESVSQRRKLSSVEGEDLDVYGVHVAGGHAAVAVLVMRGGQI
                     LDRRELFWEGVGAIAPERLLSEVLPQIYDRTTFIPKEVHLPVAIEGEEALCDWLAEKK
                     GERVYIRMPSRGPKAERVSLAMHNAELAYKRRFRGIQVTPGATALERHLELSEAPRRI
                     EGFDISHFQGGETVASMVVWEEGKMKKSDYRSFNIRGLTLPDDFASMRQAVERRYRRQ
                     LEEIGEMPDLILIDGGRGQLNAALAALAELGVEETPIVALAKREEEIYTPDAPQPLRL
                     GRDDAGLRLLQQIRDEAHRFAVAKHRRRRSARTLHSRLDDLSGIGPRRRKLLIQTFGS
                     VEGVRNASAEDLQGALGPAMGRRVFAQLHPPEEGPDLVPSEAVESVPAPE"
     gene            complement(18863..20851)
                     /gene="uvrB"
                     /locus_tag="VEL74_24680"
     CDS             complement(18863..20851)
                     /gene="uvrB"
                     /locus_tag="VEL74_24680"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HYG65801.1"
                     /translation="MAGFQLVSRFTPQGDQPDAIQQLAEGVMRGDREQVLLGVTGSGK
                     TFTIAKVIEAVNRPTLVLSHNKILAAQLFQEFRSFFPHNAVEYFVSYYDYYQPEAYVP
                     ASDTYIEKETSINEEIDRLRLRATKSLFERRDVIIVASVSCIYGLGSPEAFFGMLQFF
                     SVGSNDGMDVALRRLADMQYQRTQLDLYHGAFRVRGDVLEILPAYEELGIRVEYFGDE
                     IERISRFDVLRGEAVQEVDRIAVYPKTHYVTPKDRMERAIEGIKAELRERLDLLEAEG
                     KYLERQRLEQRTRFDLEMLKELGYCHGIENYSRHLSGRAPGEPPPTLFDYFPDDFLLV
                     VDESHQTIPQVRGMYHGDRSRKQTLVDFGFRLPSALDNRPLNFEEWDARMGQRIYVSA
                     TPADWELQRTGGLISEQVIRPTGLLDPVIEVRPAQGQVDDLIGEIKKSIDLKQRVLVT
                     TLTKRMAEELTQYLEELGIKVRYMHSDVETLERVEIITALRRGDFDVLVGINLLREGL
                     DLPEVALVAILDADKEGFLRSTTALIQTSGRGARNVNGRVIFYADHMTDSMRRAIDET
                     ERRRVKQKEYNERMGIEPTTILKDIHNPLVQMQNLDLYAPGPERLLGGDGEDQPLTER
                     ISKLEKEMRAAAKRLEFEEAAQLRDKIRELREIQIYAG"
     gene            complement(21007..21495)
                     /locus_tag="VEL74_24685"
     CDS             complement(21007..21495)
                     /locus_tag="VEL74_24685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65802.1"
                     /translation="MSEPGSIPPPPPPVSPFQPRPVEPVPSRGGCGKPVVIGCVAALL
                     LGAIVLLGGIWYVANNPDVLNKMMNLSLSQLETGLMQQLPPGVTAEEKARLQAAFAGA
                     KEAIQSGRPSPEELQEFNMQLFPYSRKAGNMTRQDVQDLTELLERLARGEPTGSGGRA
                     TS"
     gene            21586..24177
                     /locus_tag="VEL74_24690"
     CDS             21586..24177
                     /locus_tag="VEL74_24690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HYG65803.1"
                     /translation="MNYDPLPEERRPPSAPPTRRPPRRPRPRLVRWAWPLLVALLFGA
                     VSGVAVAAAIHMPQVDTLVDWQPNLVTHFYDRNGQVFANFSRERRVVLKLEEISPIMR
                     QAVLASEDANFFQHGGVDLQGVARAAVADLRHGRIVEGASTITMQLARMLFLSRERTW
                     RRKVEEAFTAVELEKSYSKEQILTLYLNLVNLGHGNYGVEAASRYYFSKPAKELTLTE
                     AAMLAGILPLPSRYSPYRQPEVVLSQRDRVLRRMYEERYITRQQYEQAVAKRDLGVTT
                     QQGEEEQFAPYFAEDIRKYIETKYGANTLYESGLRVTTTLDPQIQKDTEEGVRAALLR
                     LDHRRGWRGAVAHLEAADLEAERLPTWGRGKPVPGRWYQGIVLEADATAQVKIGDEVY
                     TLDKQGIAWTNRSSPGAILKRGDVAWFRLEVPAPKKDAKKDGQEPAPAGEPKLMLEQE
                     PRMEGAAVVIESNTGAIRAMVGGWDFDRNKFNRVTQAKRQVGSAFKPLVFGAALEAGW
                     TPSDTLLDAPTSFRGGDGRLSYRPENYYKKHYGIVTLRRALEQSINVPAVKLFDLVGG
                     KKVVDFARRCGIKTNIPTWPSVALGSTDLIPLELAAAFATVANQGVHIEPYMREKVVT
                     ADGQILEQHFPASYTAMDPNVAYVLTHMMEGVIDRGTAFELKSLPIDIAGKTGTTDDF
                     SDAWFVAYTPRYTILTWVGHDVKKSLGSGMSGAIAALPMWKRIAEAGLESGWIKEGEK
                     FPVPAGVTFRETEYYSGLLKASAGAPAPSTAAGDRGLQEAFVAGTEPAREHSSKWSTI
                     TSLPWYQQKQFYIPKAGENMPGQKPEDAAAGQPPPAEGDTAPQPGAPQEDPGPQEQPA
                     EPPPPGR"
     gene            complement(24205..25464)
                     /locus_tag="VEL74_24695"
     CDS             complement(24205..25464)
                     /locus_tag="VEL74_24695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG65804.1"
                     /translation="MAADDRGVTERPAPTIDDFLVEPTRNLDDWRWLWKGDHEFPVRS
                     HRGIAGRLLVLWKRLLRPLVKVPQNDLWERQRTFNLILLEYLRKREELHHDEREELAG
                     RRYDARLEYLEELGATGIREIMRHNDAVFARLDQKVDRYRREARDLLGSLSAAVAIAE
                     TTGTPASGEQTSPAPVPSPDTVARAHEEHGYFVLERRYRGTEEEIRERISRYLPYLRA
                     VRERGPVLDLGCGRGESLALLRDEGIAARGVDSSARMVSVCRERGLDAAEGDLFETLA
                     AVPAGSLGGVVSFHVIEHLPGPMLGRLVHLAWRALAPGGVLILETPNPLSLVVAARNF
                     WLDPTHQRPVHPESLKLLYDLAGFDPIERLDLRPFPGAERLPEIDLAHLPEEQRLLAD
                     RVNRLRDRIDELLFGFQDYGMVGTKPE"
     gene            complement(25505..25951)
                     /gene="rplI"
                     /locus_tag="VEL74_24700"
     CDS             complement(25505..25951)
                     /gene="rplI"
                     /locus_tag="VEL74_24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HYG65805.1"
                     /translation="MRVILLSDQRHLGRRGEVVEVKPGYGRNFLLPQGFALEATPGNI
                     KYFEQQRSKIDARHAKERDAAAEIAAKLGGLRLEIPKRVGETDTLYGSVTAGDVAELL
                     ESKGFTVDRRRIDLEGGIKTLGDHSVRVELHPEVVAEVTVSVVRED"
     gene            complement(25962..26195)
                     /gene="rpsR"
                     /locus_tag="VEL74_24705"
     CDS             complement(25962..26195)
                     /gene="rpsR"
                     /locus_tag="VEL74_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HYG65806.1"
                     /translation="MGRPKKVFFRRRKVCKFCADKIDYIDYKDVKLLQQFIPERSKIL
                     PRRISGTCAVHQRPLQAAIKRARHLALIPFTTD"
     gene            complement(26198..26608)
                     /gene="rpsF"
                     /locus_tag="VEL74_24710"
     CDS             complement(26198..26608)
                     /gene="rpsF"
                     /locus_tag="VEL74_24710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HYG65807.1"
                     /translation="MRTYELIFVVDPRVSDEDVVTLTNDYKTMITAGGSEITSEENWG
                     RRKLAYPINKINEGKYVLLNITSQDGKTSLAEVEHRMRQNDKILRYLTVRTDEDLKRA
                     ARRKRPASAGGSSAGSTEPVDGPGNSDVDDEEEV"
     gene            complement(26766..27362)
                     /gene="pth"
                     /locus_tag="VEL74_24715"
     CDS             complement(26766..27362)
                     /gene="pth"
                     /locus_tag="VEL74_24715"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HYG65808.1"
                     /translation="MADIRLILGLGNPGERYSDTRHNVGFRVIEELARRRGVRVDRLE
                     CNSLIAEIAEVPAEEGGAPVLLAMPQTYMNRSGYAARCFVERRELEPTSVLVVYDEVN
                     LPLGKLRLRKSGSPAGHRGLESILENLRTDQVPRLRLGVAGPGGPPPGGDALVEFVLG
                     PFAPEERETAEAMIRRAADACDCWLREGAEAAMGKFNG"
     gene            complement(27410..28048)
                     /locus_tag="VEL74_24720"
     CDS             complement(27410..28048)
                     /locus_tag="VEL74_24720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HYG65809.1"
                     /translation="MSEMTIEVERREGAGKNDSRRSRGTGKIPAVVYGGGKESVSILV
                     NRKKMLELLKGKAGENPIFLLKMAESGQERHAMVRDIQVDPVSRQVIHMDFQRVLMTE
                     KVRVQVPIELTGTAYGVKTQGGMLDFVTREVHIEALPADIPHRIELDVTELRTGQHVE
                     ASALQLPKGVTLLDEPERVIVSLTHGRTEDEAGEGSDRAEPEVIKKGKTDEA"
     gene            complement(28070..29011)
                     /locus_tag="VEL74_24725"
     CDS             complement(28070..29011)
                     /locus_tag="VEL74_24725"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HYG65810.1"
                     /translation="MPGEMKIFGGRSHPALVQEICDSLGVAPGRVSAFNFSDGETFCQ
                     IDENVRGSDVFVVQPTCEPVNENLMELLIMLDSFKRSSASRITAVIPYYGYGRQDKKD
                     RPRVPITAKLVADLISRAGADRVLTMDLHADQIQGFFDVPVDHLFAAPVILDAVRDLE
                     IPDLVIVSPDAGGVERARAIAKRMEVGLAIVDKRRTAPNEAEIMHVIGDVDGCNTLIV
                     DDIIDTAGTLCKTVDALKAKGAQRVLAAGVHGILSGPALQRIEASPLESVLITNTTPV
                     DKKLARCNKVRYRSVAPLLGEAIRRIHENSSVSSLFV"
     gene            complement(29088..29162)
                     /locus_tag="VEL74_24730"
     tRNA            complement(29088..29162)
                     /locus_tag="VEL74_24730"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29128..29130),aa:Gln,seq:ttg)
     gene            complement(29405..29653)
                     /gene="spoVG"
                     /locus_tag="VEL74_24735"
                     /pseudo
     CDS             complement(29405..29653)
                     /gene="spoVG"
                     /locus_tag="VEL74_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
     gene            complement(29982..30722)
                     /locus_tag="VEL74_24740"
     CDS             complement(29982..30722)
                     /locus_tag="VEL74_24740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65811.1"
                     /translation="MRTLLGLCATLALLTGCATGPSPRPLPGVGIPPWEMPDGAYGSQ
                     RLYRVGYSGPEGEGSFRVTLRLAAADRYQVQAVDPVGRALWSLDVTGGKGLSLNHRGR
                     TFCAFEGSFDLSGVALGPFPLLSLPSLLLGRVPAQPAAAPNQQGNELTFNDGGGRRWT
                     ATVRDDGVVESWTLWEQGAPSVWWLRREDWAILSNRERRQVRWREVLQEPLANPPAPL
                     EAPAAFREECGDDSGLRELAEEPEEPPG"
     gene            complement(30739..32721)
                     /locus_tag="VEL74_24745"
     CDS             complement(30739..32721)
                     /locus_tag="VEL74_24745"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG65812.1"
                     /translation="MVRPAARLLAGLLSWSALVPAMAGAAAPSDPAALSKEASYQFAI
                     AKALASEGEMQPALEAYEKAEDLAPDAPYILVDHAQFLARVAQSAQSADEQRRLLRSA
                     TDKVERARRLAPENPDVLRAVGAIYLDLSAQDPGALAKAMEAFEAVYRQGRADPQMAL
                     VLGRLYLDQGQAEKSVEVLSALSHNLPQNRTVYLFLVEALMRAEKMAEAERTLRDLLA
                     FAPESLEARLTLADLQSRRGDNQAAAQTLSAAPESVRADPRIGRQLAWALYLSGDLAG
                     ALRTVEPLLGSEPGDRRMSLLKGLVLAAEGRNGEAADLLIRVRQAQPDDSTLARSLAQ
                     VLQRAGRNEEATAVLADVAGRLAKAGKTEEERTVRLDLAQAWFDMEKWDQGVQALAPL
                     LAAEPAVRTEALLLQADALLRAKRYDEALGLLVRASDTPQVVSKRAEVLMRAGREREA
                     EELLARIAVAPGGAATIDRQDVLSILAAAQAYQRAERYEASIPLLQRLQQLHPDDLGS
                     GFLLGTAYERTGRHQEAITLFRRLLEIQPDFHAALNYLGYIWAERGENLEEALSLARR
                     AVALAPDNGAYVDSLGWAYYRLGRFEQAREALERAARLEPGDATLQEHLGDVYVALGQ
                     TDKALEVYRRALELGDDNAEQVRRKLDRLQASPRSR"
     gene            32885..34225
                     /locus_tag="VEL74_24750"
     CDS             32885..34225
                     /locus_tag="VEL74_24750"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65813.1"
                     /translation="MGKLGQLLVARGWITVQQLTRALKNQSVVGGRLGTCLLEMDALS
                     EETLLKALAEQLGVPSAGPDDLRGVPTEVLELIPEKLARRCRAVPFQVMGSRVDVAMM
                     DPRNISCQDELAFASGKRIKVHIAHELRLFEALERYYREEMPSRLTLLLDRLNRARYM
                     WEKPAAEPDLASAAPVRRLDDLYVTTPSLAPALPGPSLPPSARAARPVTAPLRPATPA
                     AAAAPAAPAAESVPAPAPRVIPRSLTLTAEERAELGFLKEEPPPSLPPPAPPAPEAPP
                     RPKPSSTTLEEVESAFATANEREEVAEILVAFLARSYRRVALFQAGKDKVSGWLAQGE
                     GIERKVFEQFSVGFDQPSLFLNLRQGAGLHMGPMPPMPAHRALARAWGGELPKDCVML
                     PIRMKDRLVAVLYADGGRKGVAGVGLPQLQRLTAATAAAFERCILHKKRGASKS"
     gene            34295..34561
                     /locus_tag="VEL74_24755"
     CDS             34295..34561
                     /locus_tag="VEL74_24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HYG65814.1"
                     /translation="MIKSDIVDRVAEITDVPRVKAAQAVDTIIEAMKNALCDGKRIEL
                     RGFGVFQVRDRKKGVGRNPKTGVEVAISPGKTVRFKPGKDLKNL"
     gene            34674..35687
                     /locus_tag="VEL74_24760"
     CDS             34674..35687
                     /locus_tag="VEL74_24760"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HYG65815.1"
                     /translation="MLPPSGTTPVPGIHVPGEERPYRPRYLRAAILFLLTFFTTTTLG
                     PAVYLWTRTDMTTDLLPWMSPGVIRGVWSDPFLLRLGLSFSVPVLLILLAHELGHYIA
                     CRRYGLSATLPYFLPLPLMLGTLGAFIRIRSPIRGKRELFDVGVAGPLAGFVMLVPFL
                     LYGVARSEPALVEPGIGSLLVPGHCLALDLAVRLFHGPLEEGMVMNFHPFALAAWFGL
                     LATAINLIPLGQLDGGHILYAAAGRWQRRLAVPIWLLLAVAGFSWSGWWVWCVMVLLM
                     GLFHPPVRNERQPLDRKRILLAWVALAVLILSFIPRGVEEYQTPTRPGPGEGSGVFVA
                     LRP"
     gene            36053..36898
                     /locus_tag="VEL74_24765"
     CDS             36053..36898
                     /locus_tag="VEL74_24765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65816.1"
                     /translation="MPGDRRRLSTLRSAVALPLLSALLLAASGCATTRQPRSLEELWK
                     EYEQAVEAAKYPAPGRVSRSLIAITTFTPGLVWDESGQKVLMATWTKAKYYTGQPPYE
                     TTVPAPVWLTAVPFLQGFCQRSGLQGESLRVRLAERLGMPPDSANDVFVQMWVDPQDF
                     FRPCPDPEVNDSQCQVNLTTGPVDRSADCPWSAALAGQVSGNFVSVTQSQIEWMCRNW
                     EKSYPAGEPRKSYPWTALGYTYDWGAKSFYGESEFVLPPDSTVTVQSITPMDAYCARA
                     GSRAR"
BASE COUNT         5548 a        13041 c        12829 g         5680 t
ORIGIN      
        1 tccttcagcg cttctcccgg ccggccgctc actatcagcc aacgcacccc ggtgtcatgg
       61 gccgcctcca gcgtctcgcg gatcttcccg tcgcgcgaaa gctcgccgag ggacagcccc
      121 tgggagcggg tcccctgctt ctccaggagg gcgcggagca gggccgactt gccggccccg
      181 ggccagcccc agacctgcac cagcggcgcc tccagagatt cgagccggcg gaggaggcgc
      241 tcggtttgcg gcaggagata aagcggctcg tccatcctta cagcatccca atgacatccc
      301 aaaaggaaac gggggagagg ccgcgaagct accacagacc ggggtgacag cctacccgga
      361 gaggcctggc ctatctccgc aggagggctt tcagctcgcc ggccgagacc tcggaatcat
      421 tggaggcccg ctcggcgagg cccgccgcca gcacttggcg ccacagggca gcgaagcagc
      481 gcgtagtgat cttgccgatg ctcgacatcc ggtagcccag cttctccgcc acctcgggcg
      541 cctcgtaccc cagacggaag cggagccgga acagcgactg gcacttcgcg gggagccgct
      601 cgatcacccc cgccaggtcc gagagcaagt cggccttctc ctgcggcgga tccaccggct
      661 gcgacaacca ctccagcagc gtctcgtcca ccgccgcgta gatccggcgc cggcgggtcc
      721 gccagtacat caggcagtgg cggcgcaggg tccccaccag ccaggcctcg gggtcgcgga
      781 cccggtccca ttgatagagg agcgccagca gcgcctgctg aagaaggtct tcagcgtcgc
      841 cgctcgggac gcggtacttg acgaggatcc gcttgagcgt cggctcgacc ttcttcagga
      901 agtcgtcgaa ggaggagcct gcctctgggc tttccatccg ttccatgaga gccgaccaca
      961 gggtagtcct ccgtcaaagt agcagttctg ctactgactg tccagcttat agatccattg
     1021 aaaatcaaga ggatacgagg gctcagacgc cttcgcgcag gtggtcccgg acctgctgga
     1081 ggtcttccca ggtgggacgc ttgagcgccg ggttccgcag cagggcggcc gggtggtagg
     1141 tcaccatggc cggcactccc tggatggtgt accaccggcc gcgcatctgc ccgatgggac
     1201 tgtcggtgcc caacagcgcc tgcgccgcga tccggcccag tgccacgacc acccgcgggc
     1261 ggacgagcgc gatctgccgc tccaggtagc cgcggcaggc ggcgacctcg tctggctggg
     1321 ggtcgcggtt gccgggcggc cggcatttga cgacattggc gatgtagacc tcgtcacgcg
     1381 tcatctccat ggcctggatg atgcgggtca gcagctcccc cgccgcgccg acgaacggca
     1441 gcccctggcg gtcctcttcg gccccggggc cctcgccgat gaacatgagg tcggcgttcg
     1501 gattgccgga gccgaagacc accttgtggc gcccctcggc cagccggcag cgggtgcacc
     1561 cctcggtgga cgcccccagg gccgcgaggg cggcggcgcg ttccggggcg tcgtccgccg
     1621 cgacaggaag tgcgggcgcg gcgggctccg gggaggccac ctccggggaa gccatctctt
     1681 ggatggcggc ctcctggggg atggcggcgg gctcgggcgc caccaccgtg gccacgggag
     1741 ccctgggggc ccggggccgc aggtagacgt ccccgtaacc gatctcgccc aggtaggcga
     1801 ggaggtcaga caacggggcg gacggcggtg cgctcatggc tgggaagaag gacggcgaag
     1861 aggtccagca actggccggc gatggccgac ttggggctgc gcggcacgaa gaggggcggg
     1921 ccctcgcgac ggaacactgt cacctcgttg gcctcgctgc cgaaggcgat gtcggggcgc
     1981 gagacgtcgt tggccaccag gaagtccacc cccttcgctt ccatcttgcg gcgggcgttg
     2041 gcctccaggt cgtcggtctc ggcggcgaag ccgacgcgca ccgcgtccgg ggcgaccgcc
     2101 acgagcccgg ccaggatatc ggggttctcc tccagctcga tggcggcgag gccctgggtc
     2161 ttcttgatct tacgggcggc ggggcggctc ggccggtagt cggcgaccgc ggcggacatg
     2221 atcaccaggt cggcctcggg cgcgtgctcg cggaccgcgc gctccatctc ccgcgccgtg
     2281 gtgacgtcga tccgccgcac cccgggaggc gtggcgagcg ccaccggccc cgcgaccagc
     2341 accacgcggg cgccgcgccg cgccgcttcg gcggccaggg cgaagcccat cttgccgctc
     2401 gaccggttgc ccaggaagcg caccgggtcc accggctcat gagtggggcc ggcggtcacc
     2461 agcaccgtcc gcccggcgag cggcccgcgg ccggcgaccg cctcgacggc ctcgacgatg
     2521 gcgtcggggt cggccatccg gcccatcccc acctctcccg aggcgagggg gccctccacc
     2581 ggcccggcga accagacgcc gcggcggcgc agcgtgtcca cgtgctcctg gacggcgggc
     2641 tggctccaca tgacggagtg catggcggga gcggccacca cggggccggc gaaggccagg
     2701 gccgtggtgg tgaggaagtc gtcggcgagg ccgagggcca gacggccgag gacgtgggcc
     2761 gtggccggcg cgacgcacag cacctccgcc caggcggcgg cggtgatgtg cgcctcctcg
     2821 ccggagccgg tcggctccag gtactcctcc tggtggacgg cgtggccgga gaggacctcg
     2881 agggtcagcg gcgtgacgaa ggaggcggcg ctacgcgtca gcgcgcagcg cacgtcgtgg
     2941 ccgcgcgccc gcaggcgccg gaccagctcc gcggccttgt aggccgcaat gccgccgctg
     3001 acgccgagca ggacgcgcac gggcggctca gtggacctcg gcggcggcgg tctcgccctc
     3061 cgcctcggcg gtctccttcg gcggcggcgg gccgtagccc cagtcgaccg ccttctggtg
     3121 gatctcttcc atggcgacgg tggtggattt gcgcttgccg gcctcgacct tggcccgggc
     3181 accgcgcatg agctgctcgg cgcggctcgc ggccacgagg acgagacgga acttgctgtc
     3241 gacggtcggc ggagtcttgg gttccatgtt caaacagtct cctcggggag cggacatcgc
     3301 cgccaccctg gatggcagaa ggtctaggcc tgggaggccg gagcccgttc ttgaaagtct
     3361 aggaggatgg cctggatccg gggaagcatg cgatcacggc gatggcgctt ctccaggatg
     3421 atggccgcca ggacgtcgct cgcacggcgg gcatcgtcat tgatgataac ataccggtac
     3481 tgctcgaaac gacgcacctc ccgcagcgac accgcgagac ggcgggcgat cgtctgctcg
     3541 tcgtcctggg cccgccggcg caggcggctc tccagatcct cgtagctcgg gggcatgacg
     3601 aagatgccgt gcgcctccgg atgccggtgg agcacccgct cggccccctg gacgtcgatg
     3661 tccagaagca cgtcggtccc tttcgccagc cgcgggaaga cctcgtcgtt ggccgtgccg
     3721 tagaagttgc cgtgcacctc ggcccactcg aggaagcggc ctgcgtcgat catgccgcgg
     3781 aaggtggcat ggtcgacgaa gtgatagtcc cggccgtcga tctcggcgcc gcgcggcgcg
     3841 cgggtggtgt agctcaccga gaacgccaga tcgcccaggc cggcgaagct cccctgcttc
     3901 agaccctgga tcaggctctg gatgagcgtc gtcttgcccg cgccggaggg ggcggagagg
     3961 atgaagagct cgccggctga ggtcattgtg ctcaaggagc cccgattcta tctcactcga
     4021 cgttctggac ctgctcgcgg atctgctcgc agagcacttt ggcgtccagc acggcacggg
     4081 tcatctcggc gttgcggcac ttggccccga gggtgttgag ctcccggaac acctcctggg
     4141 tgaggaagtc caggcgcttg cccgtagggc ccggctccgc ggccacggac cggaagtgct
     4201 ccaggtggga gcgcaggcgg tcgatctcct cgctgacgtc gctacggtcg atcagcagag
     4261 cgatctcctg ggccacacgc gcctcgtcga cggcacggcc gtcgagcagc tcgccgacgc
     4321 ggcgccggaa ggtggccgcc atctcctcgc gcaccgggcc gcgcagctcg tcgagccgcg
     4381 ccacgatctc cgccaggccg cgcaggcgct cctccatggc cgccgccagg ctcgccccct
     4441 cgagcaggcg gccggagaca agctgggaga cggcgtccct caccaccttg agcagcagct
     4501 cgtgatccgc ctcgtcccac tcctccgcct cgacgtcgac gcggaacgcc tccgggatgc
     4561 gcagcaggtc gccggcgccc aggccgcgct cgatcagccc ctcctcgacc agccggtggg
     4621 tggcggcgtg ggcggcgcgg gcgatcccga gatggaactc caccgtcgca tggcgctcgg
     4681 cgcgcggccg ggcgtcgatc cgcacctcga cgcggccacg cgagagctcc cgtccgagca
     4741 gctcccgcac cgccgactcg ctcgaccgga tctcgtcgcc gatccggagc gccaggtcga
     4801 ggaaccggtg gttgaccgcc ttgaggctca ccgacacgat gtgccgcgcg ttctcggccg
     4861 aggcctcgcc ataccctgtc atactgcgca tcgaatcccc ctgttcattc agatcctccc
     4921 cccgaccgcc cggccagccg ctcggcctcg gcctcggcgg cgtccaggcg gcgcttgagc
     4981 tccgcggcgg ccgcggccgg atcggcccgg cgctccacgt acagcggctc gccgtagacg
     5041 tagtgcacgg tggaaaacgg cagcggcacc accatgcggt cccaggagcc caggcgccgg
     5101 gcccgggtcg ccgccagcgc cgccggctgg atcgggaacc cggacatcgc cgcggcgatg
     5161 atcaccccct gctgcacctc ccctgccggc ccgcgcggcc cgtccggcgt gaagcccagg
     5221 tccgagccct ccgccgccat gcgcagcagc ccgcgcagcc ccgccgcgcc gccccgggtg
     5281 gtggagccgc ggcaggtgtc gatccccagg cgggcgaggg tgcggaccat gatctcgccg
     5341 tcccggctgt ggctcgacag caccgtcatc cggcgccccc ggtagccgta cggcatcagc
     5401 agcatgtggc ggtgccagaa cgccaggatg aagtgctcgc cccgctgctc ccactggcgc
     5461 accagctcgt cgccgtgata gcgccgccgg acgctcgccg ccaggccgcg gatgaacccc
     5521 gcgcccagca gggagaggat ggcgatatcc ctgctcacac ccggaactgc agatcgtgga
     5581 gccgcttgta ggagccgccc agagccatga gctgttcgtg ggagccctcc tcgacgatgc
     5641 gccccgcctc catcaccgcg atgcggtcgg cgcgctgcac ggtcgagagg cggtgggcga
     5701 tgaccagcgt cgtgcgcccc tgcatcaggt tgtagagcgc cttctggacc agcgcctcgg
     5761 actggctgtc gaggtgggag gtggcctcgt cgaggatcag gatgggggcg ttcttgagca
     5821 gcgcccgggc gatcgccagg cgctgccgct ggccgccgga cagccgcacg cccgactcgc
     5881 cgatcaccgt ctcgtacccc tgcgggagct gcatgatgaa ctcgtccgcg tacgccgccg
     5941 ccgcggcctc gcgcacccgc tccagcggca ggtcggagcg cccgtaggcg atgttgttgc
     6001 ggatcgtgtc gttgaacagc accgtgtcct gggtgaccat gccgatcagc gaccgcaggc
     6061 ttttgagctc caggtcccgg atgtcggtgc cgtcgagcgc gatccgacct gtgtcggggt
     6121 cgaagaagcg cggcagcagg ttgaccatgg tcgacttgcc ggcgcccgag gggcccacca
     6181 gggccacgat ctcgccggcg cggacggcga ggtccacgtc ccacagcgcc ggccggtcct
     6241 cgtaggcgaa ggagacgcgc tcgaaggcga tccccttctc cacccgctcc accgtccgcg
     6301 cgccgggccg ctgctcgatg tcgttgggga gctccaggag gcggctcacc cgctgccccg
     6361 ccgccgaggc ctcctgcagg atcaggttga ccttgttcag cttgcggatc gggtcgtaga
     6421 gcatgtagag ggtgaaaagg aactgcacga gctgcgtcgg cgtcagctcc ccggcgcgga
     6481 tgctcttccc ggcgtagatc agcagcgccg ccgcgccgaa cgccgccagc gactcgacca
     6541 ccggcccgga agcgtgggtg agcatctgcg cccagaggtt gacccgcagg tggcggcgcg
     6601 tcgcctcgcg gaagcggccg tactcgaact cctccatgcc gaacgccttg accacgcggt
     6661 ggccgcggat cccctcggcc atcaggctcg ccaggtcggc catgcgctcc tggctgcggt
     6721 ggctggtgcg gcgcatcccc ttgccgaagc ggacgatggg atagaccagg accggcgcca
     6781 ccaccaggga gatgaccgcg agccggaaat gggtggagag cagcaggccg agcaggaaga
     6841 gaagggtgat cgactgctgg aagaggtcga gcaggcggtt cgacaccgcg ttctgcatca
     6901 ccgccacgtc gttgatgatg cgcgccacca gctccccgga cgggtgctcg gcgtggaagc
     6961 ggctcgactg gccgaggatg tgccagtaga ggtcgttgcg gatgtcggtc gtcaccccca
     7021 ggccgatgtg ctggaaggcg tagccggaca ggaagtcggc caggctgcgg atcaggatga
     7081 cgaggacgaa gagcaggggg acgaagaaga ccacctcgtc cggccccacc cccagccggc
     7141 gcttgagcga ttcgtagctg tcgtcgatct ggtcggcgac gtcgaagcgc ttcttgaggt
     7201 cgtcgaggaa ggccggctcc cccggcggcc tggccgcatc tcctgcggcc gccgcgtctc
     7261 cggcgctgtc ccccgagccc ggcgagccga cgatccctcc cagcccgggc agctggtcgc
     7321 cgtactggag cacctcgccg aagatcgggc cgatgaggaa gctcagcccc gccatggtcc
     7381 cggcatagac caccatcgcc agggcggcga gcatcgccca gcccacgtag cgccggatgt
     7441 agtgccagaa gaaggcccgg atgctcacgc ccgcaccccc tccgccgctt ccctcgctcc
     7501 ggcatccacc ggctccccgg agaagccgag gaaggccgcg acctcccgcg ccgcccgggc
     7561 gctggcgccc ccctcgccca ggcggccgcg cagctcggcc aggccggcgc gcacctcctc
     7621 gcgcggggcg gtctcggtca gcaggcggcg ggcctcgtcg gcgatgcggt ccggctgcgc
     7681 cgcgccctgg agcagctccg gcaccacctg ccggccgagc accaggttca ccaggctgta
     7741 gtgcggcagc cgcaccagca tccgcgccag cacataggtg ccggtggcga gacgatagag
     7801 cacgaccatg ggggtcccga ggagcccgac ctcgagcgtc gcggtgccgg aagcgcagag
     7861 ggccaggtgg gagccggcga tcgcgtcgaa gcggtcgcgc gcgatgatct ccacgcgctc
     7921 caccggcagg ccggcggcca cgatcttctc ctccagcagc tcgcgcggta cggacggtgc
     7981 ctggatcagg ctcacctgca ccggcagctc gagggcgatg cggcgcaccg cctccagcat
     8041 ggtgggcagc agcgcctcga tctcactgcg ccgcgacccg gggaggagcg ccaggcggaa
     8101 cggcccctcc ggctccccct cctcccagac ctgggggagg cgcggcacct cgtcgaccag
     8161 cggatggccg acgtggacca cgttcacccc gtgcccctgg tagaaggagg tctcgaacgg
     8221 gaagagcacc agcatgcggt cgacgacctt ggcgatggtc ttgacccggc cgcggcgcca
     8281 ggcccagacc tgggggctga tgtagtagac caccggcacc ccgcgccggc gcagctcctt
     8341 ggcgagccgc aggttgaagt cggggaagtc gacgagcacc gcagcgcgcg ggcgccgccg
     8401 gtccacctgg cggaggatct cggcgaagat ctgccgcgcc cgggggagga tcttgagcac
     8461 ttcggtgatc cccaccaccg agatctcggc gctgtgcgcc acggcatcga ggccggccgc
     8521 catcagctcg tcgcctccca ggccgaacgt ggagagcccc ggcacccggc ggcgcagctc
     8581 ggaaacgagc cgcgccgcgt ggaggtcccc ggatgcctcg cccgccacga ccaggaggtc
     8641 gacccggagg tctggagacc tctccccgcc caccatcgag gtcatggaat gggcgcgggc
     8701 ggtcccggcg ggaacgggta catcaaccag gccaccagca ggccgccggc caccatcccc
     8761 aggaagagct gcaggaagag ccgggtctgc tccttgcgct cgcgccgcca gagcagggtg
     8821 aagaacaggc tcaccaccag ggcgtacagg accatcagga agaagtggct gagcatgggg
     8881 tcaatccttc ttgccgcgtc cgatgtccca ggcgtcgagc accaggagca ggttcatgag
     8941 gcccgccgtc agcaggaagg aggtgccgta ctcgtagccc ggcccgacga tgctgccggc
     9001 gtagcccatg ccgtagcgca ggaggaagta ggggatcccc atgcccatcg agccgagcgt
     9061 ggcgaggagg gtcagcggct ggttgggcag cacgcggtag aggttcccct gcaggctgca
     9121 gcccaccacg atggagacca gcaccagcag gaaaaagacg acgcctcgct gccagcgccg
     9181 gaggatgacg tgccccaggc cggggaccag ccaggccgcc accgccgcca cgagggcgct
     9241 gcccacgccg gcggccgccg tggtctgcgg ggcggaggtg ctaccgggtt ccactcagga
     9301 ctccttccgg gcgaacagcg cccgcaggcg atcgcgtgcc tggcgcaggt cctccgggac
     9361 gacccggacg tccgccagga cggtcatgaa gttggtatcc cccttccagc gggggacgag
     9421 atggaaatgg aagtgcccgg gcactccggc gccggccggc gcgccgaggt tcatgcccag
     9481 gttgaagccg tgcggctgat aggcggccgc gagcacgcgc tgcgtctcga gcaccagcgg
     9541 ccagaactcc tggcgcgcct cctcgccgct gtcgagcggc gaggcgaggt gctcgagcgg
     9601 cgccaccatc aggtggccgc tggcgtacgg atggaggttc agcatgacca gatggtgccg
     9661 ggcggtatgc accaccagcc cctcggggtc gtccggccgc ttggcggcgg cgcagaagaa
     9721 gcacgcgggg gcatccggcg tctcgtcggg gggcttatgg gaggtgatgt acgcgtgcct
     9781 ccacggtgcg aacagggttt ccatcgcgat cggcagacaa ggagagcggg cggcggctcc
     9841 ggagagccgc cggtcagcac tcgtcgttga gcagttccct cagctccttg ccgggcttga
     9901 agtaggggat gcgcttcggg gggacgctca ccttctcgcc ggtcttgggg ttgcggccgg
     9961 tccgggcgcc gcgacggcgg atccggaagc tgccgaagcc ccgcagctcg atcttctccc
    10021 cttccttgag ggagtcgacg atgctgtcga agaccgtgtt gacgatgatc tccgcgtgct
    10081 tcttggtgag ctgggtcgta cgggcgacct cctccaccag ctcagccttg gtcataccct
    10141 ccttgacggg ttccgacatc gcccctccct ggttcatgtc cgtggggggc gggccacggg
    10201 gaccccgtga cccgccggct cgagcttcgg gagactactc ctcgtcttct tccgggtgct
    10261 cttccctgac cgcgcgcttc ttggcgcgcg gctcggcctt ggcctcctcc tccccaccgc
    10321 ccacgacctg gcccgccgtc gcctggttga gctcctcgat ctcctcgtcg gtgggctgcg
    10381 acacgccgcg catcgacagg ccgaccttct tgtcgccctc ctcgatccgc aggacgcggg
    10441 tccgcaccca gtcgcccacg ctgtagaagt cctcgagacg gacgccctgt ccgacctcga
    10501 tctcgctcac gtgggcgagc ccctcgagcc cgttgtagat gtcgatgaag aggccgaagt
    10561 cggtgacgtt gaccacccgg ccgtccagga ggtcgcccac cttgtgctcg cgggcgaagt
    10621 cctcccactc gttgggcatg aactccttga tcgacagcga gacgcgctgg ttctcgacgt
    10681 cgatgttggt gatgcgggcg cggaccgtgt cccccttctt gaggacctcg ttcgggtgct
    10741 tgacccgctt ggtccagctc atgtcggaga cgtggatcag gccgtcgatc ccctcggtga
    10801 tctcgacgaa ggcgccgaac tcggtgaggt tcctgacctt gccctcgatg atcgtgccga
    10861 tgggatggct gtcctgcagc tcctcccagg ggttgcgctc ggtctggcgc agcgacaggc
    10921 tgatgcgccg ctggtccatg tcgagctcgg agacgatcgc ctggacctcg tcgcccacct
    10981 ccagcacctt ggccggattg acgaccttct tggtccacga catctcgctg acgtggatga
    11041 ggccctcgac cccctcctcg atctcgacga aggcgccgta gtcgaccagg gagacgacgc
    11101 ggccatggac gcgcgagccg atggggtact tcttgtccac cagcgtccac gggtcgtcgc
    11161 tgcgctgctt gtagcccagc gagacgcgct cggtgtcggg gtcgaacttg aggacgatca
    11221 cgtcgacctc gtcaccgacc gagaagtgct cggacgggtg gctgacacgg ccccaggaga
    11281 tgtcggtgat gtggagcagg ccgtcgatgc cgccgaggtc gatgaacacg ccgtagtcgg
    11341 tgatgttctt gaccacgccg cgcagcatgg cgccctcttc caggcgggtg agggtatcgg
    11401 ccttgcgccg ctgcagctcg gtctcgagga ccgccttgcg cgacagcacg atgttgttgc
    11461 gccggcggtc gaggctgatc accttgaact cgagctcctg tccgcgcagg gactcgaggt
    11521 tcttgaccgg cttgatgtcg acgagggatc ccgggaggaa ggcacggaga ccgacgtcca
    11581 ccgtcaagcc gcccttgatc cggtcggtaa cccgcccctt gatgaccttg gactccttgt
    11641 aggcgcgctc cacctcggtc cagcggcgca tgcgctgcgc cttggtccgg gagaggagga
    11701 cgtgcccctc gaggtcttcg gtcttctcga gcaggacgtc gacctcttcg ccgatctcga
    11761 cggtcaggtt gccgtcgcga tcggtaaact cctcgatggg caccaagcct tcggacttgt
    11821 accccacgtc gacgatgacg gagttggacg ttacgccaat gacgcgtccg ggcacgatct
    11881 caccctcggt gagattgcgc atgctctcgt catacagctc gaggagcttc tggtagtcct
    11941 gctccgagta gtcggactcg gccgtcatgg tcgggtcctg gctttgttca ctgaccggca
    12001 tagaaaaacg ttcctccttg ggctggattc aggcgcctgg cctgtcttcc ccacgagcgt
    12061 gggggttcga gctctgctta actctttgag aactcgggcc ttcgagtata gggagctgag
    12121 gggcagggtg tcaaatcgcc ggcgggaggg gggagcacac ggacgaacac ggactgacac
    12181 gggcgatcac ggacgggcac ggacgcctgg agcagggggc ggtggtgcct ccacccctgg
    12241 gagggtgggc ggtagggagt gggggaggtc aggagggggg tttccagcgc cgccgatccg
    12301 ctccagcgcc caggcggcgt gctcctgaac gagcggcgtc ccctcttcca gagcctggcg
    12361 cagcgcgggg acataccgcg cgctcccccg gttgcccatg gcgaccgcgg cgttcctctg
    12421 cagcccctcg agcttggggc gcttcatcgg gttcccgcgg aagcgctcga cgtactcgtc
    12481 gcgccccagg aggaggagcc gggcgaggtc gagctcgccg cgctcggggg ggagctccag
    12541 ctccggatgc gtggagccct ccggcagggt gttccacggg caggcctcct ggcagaggtc
    12601 gcagccgaac acccactccc ccaccatctc ccgcgcctcc ggggggaggg agccgcggtg
    12661 ctcgatggtc cagtagctga tgcagcgccg gctgtccagc cggtagggct cggcgagcgc
    12721 ccccgtcgga caggcctcca ggcagcgcgt gcagctcccg cacatgtcgg ccagggggac
    12781 atccggggcg aggtccaggg tgaggaacag ctcgccgagc aggaaccagg acccgccctc
    12841 gggatggagg agaagcgtgt tcttccccac cgcccccatc cccgcccgcg ccgccagctc
    12901 gcgttcgagg accggcccgg tgtccacgta cggccgggtg tcgcaccccg ggaacgcctc
    12961 gcggacgcgg gcggcgagcg ccttgagccg cgcgcccatg acctcgtggt agtccttgcc
    13021 ccgcgcgtac cgggccacgc gaggccagag gtcgccgtcg ggctcggcct cctccgtgcc
    13081 gtcctcctta gagagaggat ggtattggag ggcgacgaag aggacgctcc gcgctcccgg
    13141 atggacctgg cccggatcga gccgcgcctc cacccgccgc tccatccacg acatgtccgc
    13201 ctgatccccc cgctccagcc accggagcag ggacggccca tgctccaggg gctcgagcgt
    13261 cgccaccccg gcccggtcga acccggcctc gagagcccag gccttcaaca gctcggcggt
    13321 ggcgcggggg tcgagggggt ccatgggagg taggataacc ggcggcggga ggggaaagtt
    13381 cccggagcgg cggcgggccg ggaagacgat gcggatgcgc gaggggcggt ggtgcctccc
    13441 cccctgggag ggcgggcggt agggagcggg ggaggtcagg aggggggttc ttttccggac
    13501 cgccgagctg cctgcgtccc ttcgggactt tggaatcaat gatttgccct ctacctggca
    13561 ttgaaatgcc aggctttatg cctgcgccgc ttcgcggctg aaccgacttc acggccggcc
    13621 cgctcggtga gtcggggata ggataggccg atgccctata gggagaacgc gtcttgtaca
    13681 tccagcgagt gaagatcgag aacgtccgag ggttcaagga ggtggacctg gacctcaccc
    13741 gccccacggg gagtccggcg gggtggacgg tgctcgctgg ccggaatggg tcggggaagt
    13801 cgacgctgct caaggcgatg gcattggtgg cagccggaga gtggggtctc agatttcacg
    13861 agcagatgcg gctttgggtt cgccagaacg agccgcgtgc ccgtgtccgc gctttactga
    13921 gtgagaaggg gcaactgttc gaggaagaac tgacatggtc aggttcggca agcccttttg
    13981 caccagaaag cttcgcggct cccccgaacc ccgcccggcc cgacggatgc ctgatcgcct
    14041 acggccccta tcgccgcctc tccggccatg ccactgacgc tcagcgtctc atggagggat
    14101 cgtctcccgt ctcccgcatg gtcaacctct tccgcgagga cgcctccctc gtcgaatgcg
    14161 tcacctggct ccgggagatc tacctccgcc gcctcgagaa gaagcccggc gccgccgagt
    14221 tggagaaggc cgttctctgc ctactcaacg acgacctact cccggagaaa actcgcgtcg
    14281 acggcatcga ctccgaggcc ctctgggtcg tgcaacacgg cgcccgtctc cctctccagg
    14341 agctgagtga cggctatcgc accaccgccg ccctggtgat ggacatcgtc cgtcatctct
    14401 acctcacctt cggagagctc cgcatcgaga ccgccaccga cgatgaaggc accttccagc
    14461 gcgtcctcca cgagggcgtg gtcctcatcg acgaggtgga gacccacctc cacgtttcct
    14521 ggcagaagaa gatcggcttc tggctcaaac gccacttccc ccacatccag ttcatcgtca
    14581 ccacccacag cccgttcgtc tgccaggctg ccgacccggg gggcctgatc cggctgccgg
    14641 ctccagggga gaaccgcccc gtcgagcacg tgccagagtc cctctactac accgtggtca
    14701 acggcggtgc cgacgatgcc gtcctcacgg agctgttcgg gttagatagc ccctactcgg
    14761 acgaatccga gaagctccgg gaggagatcg cccagctcga agcccgggtg cagcagggca
    14821 aggcgactga aggcgacctc cagagcctcg accagatgcg ctcgcgcctc ccgcgcacca
    14881 tgaccacgga tgtggagttg gccctgaaga agctcgccgc cgacctatga ggcgcctcca
    14941 ccggactccg ctcagcgaca aaaccaggat cttcctcagg aagcgcgccg gccaggtcgc
    15001 cgacgcgctc caccccatcg ccgaggcgaa gcgtctctgg gaccggctgg acaacaaggc
    15061 ctttcgggag atccgccaga cgttggccgc catggcgagc ggcgtcgagc ggtgcatgta
    15121 ctgcgaggac agcgaaggga ccgccatcga gcacttctgg cccaaggccc tctacccgaa
    15181 gcgcgccttc gactggaaca actacctgct cgcctgctcg cgctgcaaca gcaactacaa
    15241 gcgcaaccgg ttccccctcg accctgccgg ccggcccctc ctcgtcaatc cggtcgaaga
    15301 gcaacccctc gaccacctgg ccttttcgcc gtccaccggc cggtatgagc ccagaacgaa
    15361 caaaggaagg gctagcgagg aggtctacgg actgaatcgg acgaagttga cccgcggccg
    15421 gtccaacgcc tggaccgtgc tccagaagct gatcgtcgtc tacgcccaag ccaaggaaga
    15481 gggagacctc gccgaggctg ccaagatcga gaccgccctt cgggagcacc cgttcgcggg
    15541 cgtcctcgcg gccctcctcc acctcgccgc aggacccggc gccgccctgg tgcaccccgc
    15601 ctccctccaa gcgctcctgc agaaccccga aatccagggc tgggcctgag cgccagccgt
    15661 cgtacccgga ggaaggacgg ccatccctcg tggggcagcc gttgccccgg caggaatgac
    15721 cttcattcct catggggcag ccatggcccc gacagaaacg gacaccattc ctgaaggggc
    15781 agccactgcc ccaacaggaa tgatctccat tcctcctggg gcagcgactg ccccgatagg
    15841 aacgctctcc attcctcgtg gggcaacgat ggccccgaca ggaatgacca ccattcctca
    15901 tggggcagcg gctgccccag gagggatgaa ccccgccgcc gcttccgcga cgacggggat
    15961 ctcttcctta tctaaatggg aaaggtgggc ggaccccgcg aggcgaggcc cgccgtccat
    16021 gcggcgtcag acttcgagaa aggtgacgcc cgcgggactc gacgggtccg cgtcctcgac
    16081 gatccgcacc accgaccagc gctgccaggg cgcccgcggg tccggctcct cctccagaga
    16141 gagccaggag gcataccacc ggccgcggtc gtaccagacc gtcagctcgg cggcgtcggg
    16201 accgtagtcg gggatccagt gattccccgg gtcctccttc gccatctgga tctcttcagg
    16261 agtgggagtg aagtggattc gcaccaccag gtcatacagc gcccggcggg tggattcggt
    16321 gggacgggac agatcgacgc cgttgggcgt acgatcactg tggctgggca tgagtgttag
    16381 ctcctgtccg gcaaggcctc gggaggtgag cgtcaactcc ctcccttggc cgcttggact
    16441 ctgacgtctc ggatgagaca cgcgctcacc ttggagagac agcggtcctc tacggctgtg
    16501 ctgtgaacct gcggtttcct tctctccccc taccacctcc ttcgcagttc gggacaataa
    16561 cggcggaacg gctgacgtga gcctataacc gccctagcta cgcgtcaaga ggaaaatcgg
    16621 ctttcgggaa aaaggggaag ggaccaggaa gcgggaaggg ggccgggggg tgagggcccc
    16681 ccgcagcccc gctcggtcct agcactgagg gcagcagcag gcgatcgcgc accggacctc
    16741 ggcccgatta caggcgacct cgcaggagag cctgtccgga tgaccgatcg gcggacaatt
    16801 cgccaagcag tcgaaggtcg ccaggtagca gccgcagtca tcccccgagc acgccagggt
    16861 gttcgacggg gccaccacca cggccagcag aacgacgcac agcatggcac acacgtacac
    16921 cagcttcgct ttcatggtct cctccttgga gatggttagc gaagcgaata atagcagaaa
    16981 cttaatttaa gaccgctact ccggagccgg aacgctctcg accgcctccg agggcaccag
    17041 gtccggcccc tcctccggcg gatggagctg ggcgaatacc ctgcggccca tggcggggcc
    17101 gagggcgccc tggagatcct cggcggaggc gttgcggacc ccctcgacgc tgccgaaggt
    17161 ctggatgagg agcttcctcc gccgcgggcc gatgccggag aggtcgtcga ggcggctgtg
    17221 gagggtgcgg gcggagcggc ggcggcggtg cttcgcgacg gcgaagcggt gggcctcgtc
    17281 gcggatctgc tgcaggagcc ggaggccggc gtcgtcgcgg ccgagacgga gcggctgggg
    17341 cgcgtccggc gtgtagatct cctcctcgcg cttggccagc gcgacgatgg gcgtctcctc
    17401 cacccccagc tcggcgagag cggcgagggc ggcgttgagc tgcccccggc cgccgtcgat
    17461 caggatcagg tccggcatct cgccgatctc ctcgagctgg cggcgatagc ggcgctcgac
    17521 cgcctggcgc atggaggcga agtcgtccgg cagggtgagc ccccggatgt tgaagctccg
    17581 gtagtcgctc ttcttcatct tcccctcctc ccagacgacc atcgacgcca ccgtctcgcc
    17641 cccctggaag tgggagatgt cgaagccctc gatccgccgc ggcgcctcgg acagctccag
    17701 gtgccgctcc agtgccgtcg cccccggcgt cacctgaatc ccccggaagc ggcgcttgta
    17761 ggccagctcg gcgttgtgca tcgcgaggga cacccgctcc gccttggggc cgcgcgaggg
    17821 catccggatg tagacccgct cccccttctt ctccgccagc cagtcgcaca gcgcctcctc
    17881 cccctcgatg gccaccggca ggtgcacctc cttggggatg aaggtcgtgc ggtcgtagat
    17941 ctgcggcagc acctcggaca gcagccgctc cggcgcgatg gcgccgaccc cctcccagaa
    18001 cagctcccgg cggtcgagga tctggccgcc gcgcatgacc agcaccgcca cggccgcgtg
    18061 gccgcccgcc acgtggacgc cgtagacgtc gaggtcctcc ccctcgaccg acgacagctt
    18121 gcgccgctgg ctgaccgact ccacctcgga aagggcgtcg cggagctgtg ccgcccgctc
    18181 gtagtccatc gactcggcgg cgtcccacat ctcgcgcttg agccgcttga cgagctggtc
    18241 gttcttgccc gccaggaaga gccgcgcctg ctgcaccgcc tcgtcgtacg cccccttggt
    18301 ggtcaggccg gcgacacagg ggccgaggca gcggtgcatg tcgtagtaga ggcagggccg
    18361 gggcagccgg ccgtcgatct cgatccggca gacccgcacc tggaagagct tctggaccag
    18421 cttgatcgcc ttgcgcgcca ggccgcccgg gaggaagggc ccgaagtagt ccgccttgtc
    18481 gttcgagatg cggcgggtga acgcgaggcg cgggtactcg tcggccgacg tcagcttgag
    18541 gtaggggtag gtcttgtcgt cgcgcagcag gacgttgtag cgcggccggc ggtccttgat
    18601 ccagttgttc tcgaggagga gcgcctcggc ctcggagtcg gtgaggaccg tgtcgatgtc
    18661 cgccgcctcc gcgatcatgg cggcgaggcg ggtgtccagg tcgcggttca ggtaggaggc
    18721 ggtccgcttg cgcagcgact tcgccttgcc gacgtacagg accttccccg cggcatcctt
    18781 gaagatgtag atgcccgggc ggtccggcag ctcccggatg cgctcgacga ctctctcgtg
    18841 ggccatctcg tccgcgaccc gcctagccgg cgtagatctg gatctcccgc agctcgcgga
    18901 tcttgtcgcg gagctgcgcc gcctcctcga actccaggcg cttggcggcg gcgcgcatct
    18961 ccttctcgag cttcgagatg cgctcggtca gcggctggtc ctcgccgtcg ccgcccagca
    19021 gccgctcggg gccgggggcg tagaggtcga ggttctgcat ctggacgagc gggttgtgga
    19081 tgtccttgag gatggtggtg ggctcgatcc ccatccgctc gttgtactcc ttctgcttga
    19141 cccgccggcg ctcggtctca tcgatcgccc ggcgcatgga gtcggtcatg tgatcggcat
    19201 agaagatgac gcggccgttg acgttgcggg cgccgcggcc ggaggtctgg atcagcgccg
    19261 tggtgctgcg caggaacccc tccttgtcgg cgtcgaggat cgccaccagc gccacctcgg
    19321 gcaggtcgag cccctcgcgc agcaggttga tgcccaccag gacgtcgaag tccccgcgcc
    19381 gcagggcggt gatgatctcg acgcgctcca gggtctcgac gtcggagtgc atgtagcgca
    19441 ccttgatccc cagctcctcc aggtactgcg tcagctcctc ggccatgcgc ttggtgagcg
    19501 tggtcaccag cacgcgctgc ttgaggtcga tcgacttctt gatctcgccg atgaggtcgt
    19561 ccacctgccc ctgggcgggg cgcacctcga tcaccgggtc gagcaggccg gtggggcgga
    19621 tcacctgctc ggagatcagg ccgccggtgc gctgcagctc ccagtccgcc ggggtggccg
    19681 agacgtagat ccgctgcccc atgcgcgcgt cccactcctc gaagttgagc ggccggttgt
    19741 ccagcgccga ggggagccgg aagccgaagt cgaccagcgt ctgcttgcgc gaccggtcgc
    19801 cgtggtacat gccgcgcacc tgcgggatgg tctggtggct ctcgtcgacc accagcagga
    19861 agtcgtccgg gaagtagtcg aacagggtcg gcggcggctc tccgggggcg cggccggaga
    19921 ggtggcgcga gtagttctcg atgccgtggc agtagccgag ctccttgagc atctcgaggt
    19981 cgaagcgggt gcgctgctcc aggcgctggc gctccaggta cttgccctcg gcctccagca
    20041 ggtccaggcg ctcccgcagc tcggccttga tcccctcgat cgcccgctcc atccggtcct
    20101 tgggcgtgac gtagtgggtc ttggggtaga cggcgatgcg gtccacctcc tggacggctt
    20161 cgccgcgcag cacgtcgaag cggctgatgc gctcgatctc gtcgccgaag tactcgacgc
    20221 ggatgcccag ctcctcgtag gcggggagga tctcgagcac gtcgccgcgc acccggaagg
    20281 cgccgtgata gaggtcgagc tgggtgcgct ggtactgcat gtcggccagg cggcgcagcg
    20341 ccacgtccat gccgtcgttg ctgccgacgc tgaagaactg gagcatgccg aagaaggcct
    20401 ccggcgagcc gaggccgtag atgcaggaga cggaggcgac gatgatcacg tcccggcgct
    20461 cgaacagcga cttggtggcg cgcaggcgga gccggtcgat ctcctcgttg atgctggtct
    20521 ccttctcgat gtacgtgtcc gaggccggta cgtacgcctc cggctggtag tagtcgtagt
    20581 aggagacgaa gtactcgacg gcgttgtggg ggaagaagct gcggaactcc tggaagagct
    20641 gcgcggcgag gatcttgttg tgggagagga ccagggtggg acggttcacc gcctcgatga
    20701 ccttggcgat tgtgaacgtc ttgccggacc cggtgacgcc gaggaggacc tgctcccggt
    20761 ccccccgcat cacgccttcg gcgagctgct ggatggcgtc gggttgatcc ccctggggcg
    20821 tgaagcgcga gacgagctgg aaacctgcca tggcaggatt gtaaaccgcg cggcaagggg
    20881 cgtctgttcc ccggccggcc gtgggctggc ctgtcctgct cggctccttc gtgtgcgctc
    20941 ccccctcccc tccgcccgct gccagtcgcc tgcgccggac aggccagccc acggcctccg
    21001 ccccgctcag ctcgtagccc ggcctcccga gccggtgggc tcgccgcggg ccagccgttc
    21061 caggagctcg gtcagatcct gcacgtcctg gcgggtcatg ttccccgcct tgcgcgagta
    21121 cgggaagagc tgcatgttga actcctggag ctcctcgggg ctgggccgtc ccgactggat
    21181 cgcctccttg gcgccggcga acgccgcctg gaggcgcgcc ttctcctccg cggtcacccc
    21241 cggggggagc tgctgcatca acccggtctc cagttggctc aacgagaggt tcatcatctt
    21301 gttgaggacg tccggattgt tggcgacata ccagatgccg ccgaggagga cgatggcgcc
    21361 gaggagcagg gcggccacgc agccgatgac caccggcttg ccgcagccgc cgcgcgacgg
    21421 cacgggctcg accggccggg gctggaacgg cgagaccggc ggtggggggg gtgggatgct
    21481 gcccggttcc gacatcaagg ccctccgtga gagttcgcca cctttctatc accggccggc
    21541 agggcgccac aagcacggcc tccgggaggc ctctataatg gcggggtgaa ctacgatccg
    21601 ctgcccgaag aacgccgtcc cccctccgct cctccgaccc gccgtccgcc ccggcggccc
    21661 cggccgcggc tggtgcgctg ggcctggccg ctcctcgtcg ccctgctctt cggggcggtg
    21721 agcggcgtcg ccgtggccgc cgccatccac atgccgcagg tggacaccct cgtcgactgg
    21781 cagccgaacc tggtgaccca cttctacgac cggaacggcc aggtcttcgc caacttctcc
    21841 cgcgagcggc gggtggtcct caagctcgag gagatctccc cgatcatgcg gcaggcggtc
    21901 ctcgcctccg aggacgccaa tttcttccag cacggcggcg tcgacctgca gggcgttgcc
    21961 cgcgccgccg tcgcggacct gcgccacggc cggatcgtgg agggcgcctc caccatcacc
    22021 atgcagctcg cccgcatgct cttcctctcc cgcgaacgca cctggcgccg caaggtcgag
    22081 gaggcgttca ccgcggtcga gctcgagaag agctacagca aggagcagat cctcaccctg
    22141 tacctcaacc tggtgaacct ggggcacggc aactatggcg tcgaggccgc ctcgcgctac
    22201 tacttcagca agccggccaa ggagctgacg ctcaccgagg ccgcgatgct cgcgggcatc
    22261 ctccccctgc ccagccgcta cagcccctac cgccagcccg aggtggtgct cagccagcgc
    22321 gaccgggtcc tgcggcggat gtacgaggag cgctacatca cccggcagca gtacgagcag
    22381 gcggtggcca agcgggacct gggtgtgacc acgcagcagg gcgaggagga gcagttcgcc
    22441 ccctacttcg cggaggacat ccgcaagtac atcgagacca agtacggcgc caacaccctc
    22501 tacgagtcgg ggctccgggt caccaccacg ctcgacccgc agatccagaa ggacaccgag
    22561 gagggggtgc gggcggcgct gctgcgcctc gatcaccgcc gcggctggcg cggcgccgtc
    22621 gcccacctgg aggcggcgga cctcgaagcc gagcggctcc ccacctgggg ccgcggcaag
    22681 ccggttcccg gccgctggta ccaggggatc gtgctggagg ccgacgccac cgcccaggtc
    22741 aagatcggcg acgaggtcta caccctcgac aagcagggga tcgcctggac caaccgctcg
    22801 tcgcccggag cgatcctcaa gcgcggcgac gtcgcctggt tccggctcga ggtgccggcg
    22861 ccgaagaagg acgccaagaa ggacggccag gagccggcgc cggccggcga gccgaagctg
    22921 atgctggagc aggagccgcg gatggagggc gcggcggtcg tcatcgagtc gaacaccggc
    22981 gccatccgcg ccatggtcgg cggctgggac ttcgaccgca acaagttcaa ccgcgtcacc
    23041 caggcgaagc gccaggtcgg ctcggcgttc aagcccctgg tcttcggcgc cgcgctggag
    23101 gccggctgga cgccgtccga caccctgctc gacgccccca cctccttccg ggggggcgac
    23161 ggccggctga gctaccggcc cgagaactat tacaaaaagc attacggcat cgtcaccctg
    23221 cggcgcgccc tcgagcagtc gatcaacgtg ccggcggtca agctgttcga cctggtcggc
    23281 ggcaagaagg tggtggactt cgcgcggcgc tgcggcatca agacgaatat ccccacctgg
    23341 cccagcgtcg ccctggggtc gaccgacctg atcccgctgg agctcgccgc cgccttcgcc
    23401 accgtcgcca accagggggt ccacatcgag ccctacatga gggagaaggt ggtgacggcc
    23461 gacggccaga tcctggagca gcacttcccc gcctcctaca ccgccatgga cccgaacgtg
    23521 gcgtacgtgc tgacccacat gatggagggg gtcatcgacc ggggcaccgc cttcgagctc
    23581 aagagcctcc ccatcgacat cgcgggcaag accggcacca cggacgactt ctcggacgcc
    23641 tggttcgtcg cctatacccc tcgctatacg atcctcacct gggtcggcca cgacgtgaag
    23701 aagtcgctcg gctcgggcat gagcggcgcc atcgccgccc tgccgatgtg gaagaggatc
    23761 gccgaggccg gtctcgagag cgggtggatc aaggaggggg agaagttccc ggtgccggcg
    23821 ggcgtgacct tccgcgagac cgagtactac tccggcctgc tcaaggcgtc ggccggcgcg
    23881 cccgctccga gtaccgctgc cggcgaccgg ggcctgcagg aggccttcgt cgccggtacc
    23941 gagcctgccc gcgagcacag ctccaagtgg tcgaccatca ccagcctccc ctggtaccag
    24001 cagaagcagt tctacatccc caaggcgggc gagaacatgc cgggccagaa accggaggac
    24061 gcggcggccg gccagccgcc ccctgccgag ggagacaccg cgccgcagcc cggcgcgcca
    24121 caggaagacc ccggcccgca ggagcagccg gcggagccgc cgccaccggg gagatgagag
    24181 taggggtggg tgaggagccg gaggctactc cggcttcgtc cccaccatcc cgtagtcctg
    24241 gaagccgaac aacagctcgt cgatccggtc gcgcaggcgg ttgacgcggt cggcgaggag
    24301 gcgctgctct tcggggagat gggcgaggtc gatctccggc agccgctcgg cgccggggaa
    24361 cgggcgcagg tcgaggcgct cgatggggtc gaagccggcc aggtcgtaga ggagcttcaa
    24421 gctctcggga tggaccggcc gctggtgggt ggggtcgagc cagaagttgc gcgccgccac
    24481 caccagggag agcgggttcg gcgtctccag gatcagcacg ccgccgggag ccagggcccg
    24541 ccaggcgagg tgcaccagcc gccccagcat cgggccgggc aggtgctcga tgacgtggaa
    24601 cgagaccacg ccgccgaggc tccccgcggg caccgccgcc agcgtctcga agaggtcccc
    24661 ttcggccgcg tccaggccgc gctcgcggca gacggatacc atgcgggcgc tgctgtccac
    24721 gccgcgggcc gcgatcccct cgtcgcgcaa gagggccagc gactcgccgc ggccacagcc
    24781 caggtccagc accggccctc gctcccgcac cgcccgcaga tacggcaggt agcgcgagat
    24841 ccgctcgcgg atctcctcct ccgtcccgcg gtagcgccgc tcgaggacga agtagccgtg
    24901 ctcctcgtgc gcccgggcca cggtgtcggg cgagggaacc ggagcgggag acgtctgctc
    24961 cccgctggcc ggcgtcccgg tcgtctcagc gatggccacg gcggcgctga ggctgcccag
    25021 gaggtcccgg gcctcgcggc ggtagcggtc caccttctgg tcgaggcggg cgaagacggc
    25081 gtcgttgtgc cgcatgatct cgcggatgcc ggtcgccccc agctcctcca ggtattcgag
    25141 acgggcgtcg tagcgccggc cggccagctc ctccctctcg tcgtggtgga gctcctcgcg
    25201 cttcctgagg tattcgagca ggatcaggtt gaaggtccgc tgccgctccc agaggtcgtt
    25261 ctgggggacc ttgaccagcg ggcggaggag gcgcttccag aggaccagga ggcgcccggc
    25321 gatgccgcga tggctgcgga cggggaactc atggtccccc ttccacagcc accgccagtc
    25381 atcgagattg cgggtgggtt cgacgaggaa atcgtcgatc gtgggtgccg ggcgctcggt
    25441 aactcccctg tcgtcggccg ccatggctct ctccgcaggg gtcgtccccg ggcgccccgg
    25501 gtgtctagtc ttcgcgcacc accgagacgg tcacttcggc gaccacctcg ggatgcagct
    25561 cgacgcggac ggaatgatcc ccgagggtct tgatgccccc ctcgaggtcg atgcgccggc
    25621 ggtcgacggt gaagcccttg ctctcgagga gctccgcgac gtcgcccgcg gtgaccgagc
    25681 catagagggt gtcggtctcg ccgacccgct tggggatctc gaggcgcagc ccgcccagct
    25741 tggcggcgat ctccgccgcg gcgtcacgct ccttggcgtg ccgggcgtcg atcttggagc
    25801 gctgctgctc gaagtacttg atgttgcccg gcgtcgcttc gagggcgaag ccctggggca
    25861 ggaggaagtt ccggccatag cccggcttga cctccaccac ctcgccgcga cggccgagat
    25921 gccgctgatc gctcaacagg atgactctca tcgccgctct cctagtccgt ggtgaacggg
    25981 atcagcgcca gatggcgagc ccgcttgatc gccgcctgga gaggccgctg gtgaaccgcg
    26041 caggtgccgg agatgcgccg cggcaggatc ttcgagcgct ccgggatgaa ctgctgcaga
    26101 agcttgacgt ctttgtaatc gatgtagtcg atcttgtcgg cgcagaactt acagaccttg
    26161 cgccggcgga agaatacctt cttgggccgt cccatggtca aacctcctcc tcgtcgtcaa
    26221 cgtcggagtt accggggccg tccaccggct cggtggagcc ggccgaggaa ccgccggcgg
    26281 acgccggacg cttccggcgg gcggcgcgct tgaggtcctc gtccgtgcgc accgtcaggt
    26341 agcgcaggat cttgtcgttc tgccgcatcc ggtgctcgac ctcggcaagg ctcgtcttgc
    26401 catcctggct cgtgatgttg agcaggacgt acttgccttc gttgatcttg ttgatcgggt
    26461 aggcgagctt gcgccgtccc cagttttcct cgctggtgat ctcggagcca ccggcggtga
    26521 tcatggtctt gtagtcgttg gtcagggtga ccacgtcttc gtccgacaca cgagggtcga
    26581 cgacgaagat cagctcatac gtgcgcatgg gtggttgact tcctccctat ggcctgggtc
    26641 ggtcccgctc tagacggaac agagaggtga atgattcgcg gcgccggctg acccgggccg
    26701 cgggtgcaag gacagagaat tatgcacgga accggggaaa gcgcaagccc ccctcagtcg
    26761 gccagtcagc cgttgaactt ccccatcgcc gcctcggctc cctcgcgcag ccagcagtcg
    26821 caggcgtcgg cggcgcggcg gatcatcgcc tcggccgtct cccgctcttc cggcgcgaag
    26881 gggccaagga caaactccac cagcgcgtcg ccgcccgggg gcgggccgcc gggaccggcg
    26941 acgccgaggc gcaggcgcgg cacctggtcg gtgcgcaggt tctctaggat cgactccagg
    27001 ccgcggtggc cggccgggct ccccgacttg cgcaggcgca gcttgccgag cggcaggttg
    27061 acctcgtcgt agaccaccag gaccgacgtc ggctcgagct cccgccgctc gacgaagcag
    27121 cgggcggcgt agccgctccg gttcatgtag gtctggggca tggccaggag gaccggcgcg
    27181 cccccctcct ccgccggcac ctcggcgatt tcagcgatga gggagttgca ctccagccgg
    27241 tcgacgcgca caccccggcg gcgcgccagc tcctcgatca cccggaagcc gacgttgtgc
    27301 cgggtgtcgg agtagcgctc gccgggattg cccagcccga ggatgagcct gatgtccgcc
    27361 aagggcgcct gaaggggaag cgccgccccg gggtctcccc cggaggcgac taggcctcgt
    27421 cggtcttgcc cttcttgatg acctcgggct cggcgcggtc ggacccttcg cccgcctcgt
    27481 cctcagtccg gccatgcgtc agggagacga tgacccgctc gggctcgtcc agcagggtga
    27541 cgcccttggg gagctgcagg gcgctcgcct ccacgtgctg cccggtgcgc agctcggtga
    27601 cgtcgagctc gatccggtgc gggatgtcgg cgggcagcgc ctcgatgtgc acctcgcggg
    27661 tgacgaagtc cagcatgccg ccctgggtct tgacgccgta ggcggtgccg gtcagctcga
    27721 tgggcacctg cacgcgcacc ttctcggtca tcagcacgcg ctggaagtcc atgtggatca
    27781 cctgccggct gaccgggtcc acctggatgt cgcgcaccat ggcgtgccgc tcctggccgc
    27841 tctccgccat cttgagcagg aagatcgggt tctcgcccgc cttgcccttg agcagctcca
    27901 gcatcttctt gcggttcacc aggatggaga ccgactcctt gccgccgccg tacaccacgg
    27961 cggggatctt gcccgtgccg cgggaccgcc ggctgtcgtt cttgccagcg ccttcgcggc
    28021 gctctacttc gatggtcatc tcgctcatga ttgcctcgct tctttccttc tatacgaaca
    28081 gggagctcac cgagctgttc tcgtggatcc ggcggatggc ctcccccagc agcggcgcga
    28141 ccgaacggta ccgcaccttg ttgcagcggg cgagcttctt gtccaccggc gtcgtgttgg
    28201 tgatcaggac cgactcgagc ggcgacgcct cgatgcgctg caacgccggc ccggagagga
    28261 taccgtgaac gcccgccgcc aggacgcgct gggcgccctt ggccttgagc gcgtccacgg
    28321 tcttgcacag cgtccccgcc gtgtcgatga tgtcgtcgac gatcagggtg ttgcagccgt
    28381 cgacgtcgcc gatgacgtgc atgatctcgg cttcgttggg ggcggtgcgg cgcttatcga
    28441 cgatcgccag ccccacctcc atccgcttgg cgatggcccg cgcccgctcg acgccgccgg
    28501 cgtccggcga gacgatgacc aggtccggga tctccaggtc gcgcaccgcg tcgaggatca
    28561 cgggcgcggc gaacaggtgg tccaccggca cgtcgaagaa cccctggatc tggtcggcgt
    28621 ggaggtccat ggtcagcacc cggtcggcgc cggcgcggga gatgaggtcc gccaccagct
    28681 tggcggtgat cggcacgcgc ggccggtcct tcttgtcctg gcgcccgtag ccgtagtagg
    28741 ggatgacggc ggtgatgcgg gaggccgagg agcgcttgaa gctgtccagc atgatcagca
    28801 gctccatcag gttctcgttc accggctcac aggtgggctg gaccacgaac acgtccgacc
    28861 cgcggacgtt ctcgtcgatc tggcagaagg tctcgccatc gctgaagttg aaggcggaca
    28921 cgcggcccgg ggccacgccg agggagtcgc agatctcctg cacgagggca ggatgggacc
    28981 gtccaccgaa gatcttcatt tcgcctggca tgctctctcg ctccgaacga gaatcgaggc
    29041 ccgcctcgcg aggccgggcc ccgaaaaaag aggaaccgct acgtctctgg ttggggcggg
    29101 aggattcgaa cctccggatg ccagatccaa agtctggtgc cttaccgctt ggcgacgccc
    29161 cagtctcggc cacgattcgg gacggcggca ccggccggcc gtcccttctg caccccgagc
    29221 gggccgccgc ggcagccggc ctggggcgcc gtcaggtcac ggtccagaag gagtcgcgga
    29281 gatgcatctc ctcgatctcc tccagggtct tgtcggaggt cactccgccc atcggcccgc
    29341 tcatcggcat ggagtcggcg cggtcacggg ccagaggctg cgacagggtc tccccccgct
    29401 cgcccaggac ccgctcgtac tcgccgagca ccttgtcctc gatcatccgc cgcgtctcgt
    29461 tgttcagcgg atgggcaacg tccttgaact cgccgttctt cttcttccgg ctcggcatgc
    29521 tgatgaagag gccgtccctg ccctggatga tcttgatgtc gttgaccatg aagcactggt
    29581 cgaagacgat cgagacaaac gccttgagct tgtcctcgtt gatcgggaaa accttgacct
    29641 cggtgatctc catttcgcgt ccctctccgt cagccctggt gtctctacgc cttgtacccg
    29701 catgggagaa cggccacgac ggcaggtgtt cccccggacg tggccccttc ggatgtgcaa
    29761 ccgccaccaa ggcgtcacac cgcccgaggg gccgcatcta gtcacgcgcg ggcttcaatg
    29821 gccgcattat atacctggtt ggcctccggg atacgtctag gacggttctg gagatcgctc
    29881 ggtcctgcgc gacccgacag ttggaccctg accctggggt cggggcctcc gttatgctag
    29941 ggggcggaag gggcggctgt caacccgcgg ctgcggagcg gctatccagg aggctcctcc
    30001 ggctcctccg ccagctcgcg caggccggag tcgtcgccgc actcctcgcg gaaggcggcg
    30061 ggggcctcga ggggggcggg cgggttggcc aggggctcct ggaggacctc ccgccagcgc
    30121 acctgccggc gctcgcggtt ggacaggatc gcccagtcct cgcggcgcag ccaccagacc
    30181 gagggggccc cctgctccca gagggtccag ctctccacca cgccgtcgtc gcggaccgtc
    30241 gcggtccacc gccggccgcc gccgtcgttg aaggtgagct cgttgccctg ctgattcggc
    30301 gccgccgccg gctgggccgg gacccggccg agcagcagcg acggcagcga gagcagcggg
    30361 aaggggccga gggcgacgcc cgacaggtcg aagctcccct cgaaggcgca gaaggtgcgg
    30421 ccgcgatggt tcagggagag ccccttgccg ccggtcacgt cgaggctcca gagcgcccgg
    30481 cccaccgggt ccacggcctg cacctggtag cggtccgccg cggccagccg cagggtcacg
    30541 cggaagctcc cctccccctc cgggccggag tagccgacgc ggtagagacg ttgggagccg
    30601 taggcgccgt cgggcatctc ccaggggggg atgcccacgc ccggcagggg gcggggcgag
    30661 gggccggtgg cgcagccggt cagcagcgcc agggtggcac agaggcccag gagggtgcgc
    30721 aagatagggg aggcccgctc aacgggaccg ggggctcgcc tgcagccggt ccagcttccg
    30781 gcgcacctgc tcggcgttgt cgtcgcccag ctccagcgcc cgccggtaca cctccaacgc
    30841 cttgtcggtc tgccccaacg ccacatacac atcccccagg tgctcctgca gtgtggcatc
    30901 accgggctcg aggcgggcgg cccgttccag ggcctcgcgc gcctgctcga agcggccgag
    30961 ccggtagtag gcccagccca gagaatcgac gtaggcgccg ttgtccggcg cgagggcgac
    31021 cgcccgccgc gccagggaca gcgcctcctc caggttctcc ccccgctccg cccagatata
    31081 gcctagatag ttgagtgcgg cgtggaaatc cggctgaatc tcgagcagcc ggcggaacag
    31141 cgtgatcgcc tcctggtggc ggcccgtgcg ctcgtaggcg gtgcccagca ggaaccccga
    31201 cccgaggtcg tccgggtgga gctgctgcag ccgctggagc agcgggatcg acgcctcgta
    31261 gcgctccgcc cgctggtagg cctgcgccgc cgccaggatg gacagcacgt cctggcggtc
    31321 gatcgtggcg gcgcctccgg gggcgacggc gatccgggcg agcagctcct cggcctcccg
    31381 ctcccggccg gcgcgcatca gcacctcggc ccgcttgctc accacctgcg gcgtgtcgct
    31441 ggcgcggacg aggaggccga gcgcctcgtc atagcgcttc gcccgcagga gggcgtcggc
    31501 ctggagcagc agcgcctcgg tgcgcaccgc cggctcggcg gcgaggagcg gcgccagggc
    31561 ttgcaccccc tggtcccact tctccatgtc gaaccaggcc tgcgcgaggt cgaggcggac
    31621 ggtgcgctcc tcctcggtct tccccgcctt ggcgagccgt ccggcgacat cggccagcac
    31681 ggccgtggcc tcctcgttcc ggccggcgcg ctggagcacc tgggccaggg agcgcgccag
    31741 ggtggagtcg tccggctgcg cctggcgcac gcggatgagc aggtcggcgg cctctccgtt
    31801 gcgcccctcg gcggccagga ccagcccctt gagcagggac atgcggcggt cccccggctc
    31861 ggagccgagg agcggctcga cggtgcgcaa cgcgcccgcg aggtcgccgc tcaggtagag
    31921 cgcccaggcg agctgccggc cgatccgcgg gtcggcccgc accgattccg gcgccgccga
    31981 cagcgtctgc gccgccgcct ggttgtcgcc gcgccgggat tgcaggtcgg cgagcgtcag
    32041 ccgcgcctcc agcgactcgg gggcgaaggc gaggaggtcg cgcagcgtgc gctcggcctc
    32101 cgccatcttc tccgcccgca tcagcgcctc gacgaggaag agatagacgg tgcggttctg
    32161 cggcaggttg tgggagaggg cggagagcac ctcgacgctc ttctccgcct gcccctggtc
    32221 gaggtagagg cggccgagga ccagcgccat ctgcggatcg gcccgcccct gacggtagac
    32281 cgcctcgaag gcctccatcg ccttggccag ggcgccggga tcctgcgccg acaggtcgag
    32341 atagatggcg cccaccgccc gcagcacgtc ggggttctcg ggcgccaggc ggcgcgcccg
    32401 ctccaccttg tcggtggcac tgcgcagcag ccgccgctgc tcgtccgccg actgcgcgga
    32461 ctgggcgacg cgggcgagga actgggcgtg gtcgaccagg atgtacggcg cgtcgggcgc
    32521 caggtcctcg gccttctcat aggcctccag cgccggctgc atctcccctt ccgaggcgag
    32581 ggccttggcg atggcgaact ggtacgacgc ctctttggag agagcggcag gatcggaggg
    32641 agcggccgcg ccggccatgg ccggcaccag ggcgctccac gacagcaggc cagcgagcag
    32701 gcgggcggcc ggacggacca tgagaaacaa ccccttcaca gagagaagga tagcagcggc
    32761 gcccgacgct ttacaaggct gtcgggccgc ctctataatg tccgccttgc tgttaagtgg
    32821 cgaaaaaaga ataagttagc cggggttctc cagagcaggg gcaggtatcg aggcaggcgg
    32881 acgcatgggc aagctgggac agctgttggt cgctcggggc tggatcaccg tccagcagct
    32941 cacccgtgcc ctcaagaacc agagcgtggt cgggggccgg ctgggcacct gcctgctcga
    33001 gatggacgcc ctgagcgagg agacgctgct caaggcgctc gccgagcagc tcggcgtgcc
    33061 gtccgccggc cccgacgacc tgcgcggggt ccccaccgag gtgctggagc tgatcccgga
    33121 gaagctggcg cgccgctgcc gcgccgtgcc cttccaggtg atgggcagcc gggtcgacgt
    33181 ggcgatgatg gacccgcgca acatctcctg ccaggacgag ctggccttcg cctccggcaa
    33241 gcggatcaag gtccacatcg cccacgagct gcggctcttc gaggcgctgg agcgctacta
    33301 ccgggaggag atgccgtcgc ggctgacgct gctcctcgac cgcctgaacc gggcgcgcta
    33361 catgtgggag aagccggccg ccgagccgga cctggcctcg gcggctcccg tccgccggct
    33421 ggacgacctc tacgtcacga cgccgtccct ggcgccggcg ctgcccgggc cgtccctgcc
    33481 gccctcggca cgcgcggcgc ggccggtgac cgctccgctc cgccccgcga cgccggctgc
    33541 cgccgccgcg ccggccgcgc cggccgcgga gagcgtgccc gcgccggcgc cccgggtcat
    33601 cccgcggagc ctgacgctca ccgcggagga gcgggccgag ctcggcttcc tcaaggagga
    33661 gccaccgccg tctctgccgc cgccggcacc gccggctccg gaagcgccgc cgcgcccgaa
    33721 gccctcctcc accaccctcg aggaggtgga gtcggccttc gccacggcca acgagcgcga
    33781 ggaggtggcg gagatcctgg tcgcgttcct ggcgcgcagc taccggcgcg tggcgctctt
    33841 ccaggccggc aaggacaagg tgagcggctg gctggcgcag ggagagggga tcgagcgcaa
    33901 ggttttcgag cagttctccg tgggtttcga ccagccgtcg ctgttcctca acctgcggca
    33961 gggcgccggc ctccacatgg ggccgatgcc gccgatgccg gcgcaccggg cgctggcgcg
    34021 ggcctggggc ggggagctgc ccaaggactg cgtaatgctg cccatccgga tgaaggaccg
    34081 cctcgtggcc gtgctgtacg cggacggcgg gcgcaaaggg gtcgcggggg tgggtctccc
    34141 gcagttgcag agactgacgg ccgccaccgc ggcggctttc gagcggtgta tcctccacaa
    34201 gaaacgcggt gcgtccaagt cttgagatcc cgggttccgg tttgttgacc tcccccgggc
    34261 ccattgctag actcggcgcc gcttcggagc ggacatgatc aagtcggaca tcgtagaccg
    34321 cgttgcggag atcacggacg tgccccgggt gaaggccgcc caggcggtcg acaccatcat
    34381 cgaggcgatg aagaacgccc tgtgcgacgg caagcgcatc gagctgcgcg gcttcggggt
    34441 gttccaggtg cgcgaccgca aaaagggtgt gggccgcaac ccgaagaccg gcgtcgaagt
    34501 cgccatctcc cccggcaaga cggtgcgctt caagccgggc aaggacctga agaatctctg
    34561 agcctcccca tcccccggat ccgccccggg agcaggacgg cgggctccct gccccgccct
    34621 gggctccgcc atctccacca gcggagcccc gtccgttcta cccaccggtc gaggtgctgc
    34681 cgccgtcggg caccacgccg gtgcccggga tccacgtccc cggcgaggag cgcccgtacc
    34741 ggccgcgcta cctgcgcgcc gccatcctct tcctgctgac cttcttcacc accaccaccc
    34801 tggggccggc ggtctatctc tggacccgca ccgacatgac cacggacctg ctgccgtgga
    34861 tgtcgcccgg ggtgatccgg ggggtctgga gcgatccgtt cctcctgcgg ctcggcctgt
    34921 cgttctcggt gccggtgctg ctgatcctcc tcgcccacga gctcgggcac tacatcgcct
    34981 gccggcgcta cggcctgtcg gcgacgctcc cctacttcct gccgctgccg ctgatgctcg
    35041 gcacgctggg cgccttcatc cgcatccgct cgccgatccg cggcaagcgg gagctgttcg
    35101 acgtgggcgt cgccggcccc ctggccggct tcgtgatgct ggtccccttc cttctctatg
    35161 gggtggcgag gtcggaaccg gctctggtcg agcccggcat cggctcgctc ctcgtccccg
    35221 gccactgcct ggcgctggac ctcgcggtcc ggctgttcca cggcccgctg gaggagggga
    35281 tggtgatgaa cttccatccc ttcgccctgg ccgcctggtt cggcctgctg gcgactgcga
    35341 tcaacctgat ccccctgggc cagctcgacg gcggccacat cctctacgcg gcggcggggc
    35401 gctggcagag gcggctggcg gtgcccatct ggctgctgct cgccgtcgcc ggcttctcct
    35461 ggtcgggctg gtgggtctgg tgcgtgatgg tcctgctgat gggcctcttc catccgccgg
    35521 tgcggaacga gcgccagccc ctggaccgca agcggatcct cctcgcctgg gtcgccctgg
    35581 cggtgctgat cctctccttc atccctcgcg gcgtggagga gtaccagacc ccgacgcgcc
    35641 ccgggccggg cgagggctcg ggggtgttcg tggcgctccg gccgtagccc ccaccctcca
    35701 gacgcttcgg atcgcgtccg aatgcttcgg acgcgatccg aagcggccgg gaagcgatcc
    35761 gggtctctcg gacggcatcc agacggcctg gacgagatcc gaagcgctcg ggaagaatcc
    35821 aggccttccg gatcacgtcc aaacgctctg gacgcgatcc gcagcgttcg gatgccgtcc
    35881 acggcgcccg gacgcgctcc ggcgggctcg ggagccggcc ccggctgccg aatcggctcg
    35941 gcgccggctg gatcgaggat cttgacgcag ttgctcaaag cacccaagat aggcggcgcc
    36001 acttgctcca caggtccacc ctgatacccg ggtaccaccg gaaggaggcc tcatgccggg
    36061 agaccgtcgt cgcctgtcca cgctccgctc cgccgtcgcg ctgccgctgc tctccgccct
    36121 gctgctcgcc gcctcgggtt gtgccaccac ccgccagccc cgcagcctgg aggagctctg
    36181 gaaggagtac gagcaggcgg tggaggccgc caagtacccg gcgcccggcc gcgtctcgcg
    36241 cagcctgatc gccatcacga cgttcacgcc gggactggta tgggacgaga gcgggcagaa
    36301 ggtcctcatg gcgacgtgga ccaaggcgaa gtactacacg ggacagccgc cctacgagac
    36361 gacggtcccg gcgccggtgt ggctgaccgc cgtgccgttc ctccaagggt tctgccagcg
    36421 gagcgggctg cagggcgagt ccctccgggt ccggctggcc gagcggctcg ggatgcctcc
    36481 tgacagcgcc aacgacgtct tcgtgcagat gtgggtcgac ccgcaggact tcttccgtcc
    36541 ctgcccggac ccggaggtca acgacagcca gtgccaggtg aacctcacca ccggtccggt
    36601 ggaccggtcg gccgactgtc cctggagcgc cgccctggcc ggccaggtct ccggcaactt
    36661 cgtgtccgtg acccagagcc agatcgagtg gatgtgcagg aactgggaga aatcctaccc
    36721 ggccggcgag ccgcgcaaga gctatccatg gacggccctc ggctacacct acgactgggg
    36781 ggcgaagagc ttctacggcg agagcgagtt cgtcctcccg cccgactcca cggtcaccgt
    36841 ccagtccatc actccgatgg acgcgtactg cgcccgcgcc ggctcgcgcg cgcgctgagc
    36901 ggcacgcgcc tcgcataacg tcctggtccc acccctcggc ccctccgagg ggtggtgcta
    36961 ggatgccgcc ctcccgctcc gccggagcgg tttcccacct tctacgacca aagacggata
    37021 tcgctgtgca acctggaaga gaacgacagc tcgtgaccgc ctcggtgatg ctgggcatgt
    37081 tcctggccgc gctcgacc
//
LOCUS       DASPJI010000875         8397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_569585, whole genome shotgun sequence.
ACCESSION   DASPJI010000875 DASPJI010000000
VERSION     DASPJI010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8397)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8397
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569585"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2707
                     /locus_tag="VEL74_24770"
     CDS             <1..2707
                     /locus_tag="VEL74_24770"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65817.1"
                     /translation="LAYVIYTSGSTGRPKGVMVPHRGICNRLGWAQQLYRLDERDAVL
                     QKASFGFDFSVWECFAPLWAGARLVLAEPGRQADVPYLVRVLREHRVTIAHFVPSMLA
                     AFLAEEGVETCTSLRQVFSGGEALTPELRDRALARLPGAFDNEYGPTETCVDSTRWAC
                     APGQDPRRVPIGRPISNTQVYVVDPELRPVPVGVAGELLVGGAGVTRGYLQRPGLTAE
                     RFIPDPFGGVSGARLYRTGDLVRWLPDGAVDYLGRLDHQVKVRGLRIELGEIEAALVA
                     LPGIRQAVVVVRESRLVAYAVGHAGNIGGIVVEELRRSLRERLPDYMVPAVFVMLPAL
                     PLTASGKVDRKALPAPEQPGAREEGYVAPRTREEEILAAVWAQVLRLPRVGVTDNFFE
                     LGGDSILSIQIVARARQAGLLFTVREIFEHQTVAELALHVTVADAAGAALSVQGPVTG
                     EVPLTPIQSRFFDQGLADPHHFNQALSLEPRERLSPTALDRAMAAIVEHHDALRMRFD
                     GKRQENAPAEPVTPFHQVDLSGLPASRRGEAYESAAAALQAGFELSTGPLTRLCLFDA
                     GAGQPSRLLWVTHHLVVDGVSWRVLLEDFEAAYRQAALPPKTTSFQEWARRLTGHAET
                     LIGELDYWRETARVPVPRLPVDFLMEEENLNGDEATVSFELSSEETSDLLQTLPSVYH
                     SRIDDALLSALVRALKDWTGSSRLRVDLEGHGREPLFDDLDVSRTVGWFTSMYPVVLE
                     AGDADPGEALVSVKERLRAVPGRGIGYGLLRTARLLEEAPPAEVLFNYLGQADGTAEG
                     LSLFRASTTSTGPCRSVRARRSHLLEVTGIVTAGRLQVTLTYGSRTHRRETVERLAAA
                     YAGALRELIQHSRESEEVFTPSDFPKARLDTRSFDKLAALLADDD"
     gene            2716..>8397
                     /locus_tag="VEL74_24775"
     CDS             2716..>8397
                     /locus_tag="VEL74_24775"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65818.1"
                     /translation="MKNVEDIYTLTPLQSGMLFHSLMAPESGIYVNQVTGTLPADLDA
                     RLFQQAWERLVQRHGVLRTAFLWDGLEEPLQVVRKKVALPWQELDWSGLPSEEQQRRF
                     EELRHRERHTPLPLTKAPVMRFSLIRLDGELGFIWTFHHLLLDGWSLPLLVRELVAVY
                     TALREGREPMKLLPPARPFSDYIVWLQKQDAIKAEPFWRRDLAGFTAPNPLGIAPPGA
                     ESAAGYAEHELQLSREVTAALQTLAARHKLTLQTVTLGAWAVLLSRYSNEEDVVFGNV
                     VSGRPATLPGVETMVGMFVNTLPVRVRLDGAEPLATWLRRLQERQLARQEFEHTPLAQ
                     IQRWSEIPAGSPLFETLYVFENYPDADGGGSDALRIGNLRSYESTNYPVTLAIIAADQ
                     LSLLLKTDRARVDEDAAQGLLQHLATLLAGMAEGADRPMTELPLLTAAETLQLSGWNA
                     TASDYPQACVHELFAEQAERSPDSVAVTFGGRELTYGEIGRRSRQLAHRLRRAGVEPG
                     SLVGLCAESLPELVVGMLGILEAGGAYVPLDLSYPQERLAFMLEDTGAAVLVAEEGLA
                     ERLPIHAVLRVERLGHWPAAAASREEPAFSSPGDPAYAIYTSGSTGRPKGVVVSHRAV
                     VRLVRGTDYVQIGPDDRVAQASNSSFDAATFEIWGALLNGGRLVGIERETALSPERLT
                     GALRREGVSVMFLTTALFNQVARELPAGFAHLRAVLFGGEAVDPAAVRAVLRGGPPPR
                     LLHVYGPTEATTFATWQRVEEVPPGETVPIGRPLANGTLYVMAAGIVPQPVGLPGELY
                     LGGDGLAHGYHARPELTAERFVPDPFGALTGSTAGGRLYRTGDLVRQRPDGAVEFLGR
                     LDGQVKIRGFRIEPGEVEAALLALPGVREAAVVVREDMPGERRLVAYAAGELTVESLR
                     AALRERLPDYMAPSAFVRLDALPLNPNGKVDRKALPAPERSAGAEGYLAPRTPVEEIL
                     AGIWAELLGLERVGADGNFFELGGHSLLATRVTSRLRDTLGIELPLSDVFEASSLADL
                     AARVEAARRDGTQASSPPIVPAPREGALPLSFAQERLWFIDQLEPGSPLYNIAAALQA
                     RGPLDSGVLARTLGEIVRRHEALRTAFAELAGEPVQVIQPARPFLLPVVDLSALPESV
                     REDLARTLAAAETGRPFDLSRGPLMRALLLRLGEADHAVVLTMHHIASDGWSMGILVR
                     EVAALYPAFAAHRPSPLPELPVQYADFSVWQRSWLHGEVLEQEIDFWRQRLAGLPPLL
                     ELPTDHPRPAVQSFRGAERTVRVPAGLTRRLEALARQEGATLFMVLLAGFQALLARHG
                     GQDNLAVGSPVAGRNRLETEGLIGLFVNTLVLRGDLTGAPKFSDLLGRVRETALAAYL
                     HQDVPFEKLVEELAPERSLAHTPLFQVMFTLQSFAFQRIDLPGLSFSPLAVETTTVKF
                     DLEVTVVETGDGLSAHFAFASDLFDPTTILRLSGHLLMLLETVAADPERRPGELSLLS
                     EGEHHQLQSEWNAEPALVAPGVVELFAAQVRRAPDAVAVALAGSEVSFTYRELDRRAG
                     LLAEGLRALGVGPEVPVGLCIEHGPELAAAVLGIFRSGGAYVPLDPAQPPARLAFLLE
                     DSAVPVLVTQTHLLAGLPPHGAQVVLVGGEGPVEEGGALPSPAPGDLAYRIYTSGTTG
                     RPKAVQVEHGMLASTLAATRQRFGFTADDRVSCLAQPTFDIFLFELLSPLLNGGTAVL
                     FPMRPRLDAEQVVDHLGELTTLHAVPALMRSIVDALHRRGQGAPDVSKLRRVFIGGEA
                     VPADLLEELRQAFPGAQICVLYGPTEGTILCSAHPVPPPPAPARALLGRPLPGAVLHV
                     RDAAGELLPIGVPGELWIGGPGVARGYLGREELTAEKYVLRDGQRFYRSGDR"
BASE COUNT         1128 a         2929 c         2948 g         1392 t
ORIGIN      
        1 tctggcctac gtcatctaca cctcgggctc gacaggccgg ccgaaaggcg tgatggtgcc
       61 gcaccggggc atctgcaacc ggctgggctg ggcgcaacag ctctaccgtc tcgacgaacg
      121 cgacgcagtg cttcagaaag cgtctttcgg cttcgacttc tcggtctggg agtgcttcgc
      181 ccctctgtgg gccggggcgc ggctggtctt ggccgagccc ggccggcaag ccgacgttcc
      241 atatctggtg cgcgtgctgc gcgagcaccg ggtcacgatc gcccatttcg tcccttcgat
      301 gctggcggcg ttcctcgcgg aggagggcgt ggagacgtgc acctcgttgc gccaagtctt
      361 ctcgggtggt gaggcgctca ccccggagct gcgcgaccgg gcgctggcgc gcctgcccgg
      421 ggcgttcgac aacgagtacg ggccgacaga gacctgtgtc gattccacgc gctgggcctg
      481 cgcgcccgga caggatccgc gccgggtacc gatcgggcgg ccgatctcca atacccaggt
      541 ctacgtcgtg gatccggagc tgcggccggt gccggtcggc gtggcaggcg agctgctggt
      601 gggaggagcg ggtgtgacgc gcggctatct gcagcggccc ggcctgacgg cggagcggtt
      661 catccccgat ccgttcggcg gcgtctccgg tgcccggttg taccgcaccg gcgacctcgt
      721 gcgctggctg cctgacggtg ccgtcgacta cctgggccgc ttggaccatc aggtcaaggt
      781 gcgcggcctc cgcatcgagc tgggagagat cgaggcggcg ctggtcgccc tgcccggaat
      841 tcgccaggcg gtcgtggtgg tgcgcgagtc gcggctggtc gcctatgcgg tcggccatgc
      901 cggcaacatc ggcggcatcg tggtcgagga gctgcgccgg tcgctgcgcg agcggctgcc
      961 ggactacatg gtgccggccg tcttcgtgat gctcccggcg ttgcccctca ccgccagcgg
     1021 caaggtggac cgcaaagccc tgccggcacc ggaacagccc ggcgcgcggg aggagggcta
     1081 cgtagcgccg cgcactcgcg aggaggagat cctggcggcg gtctgggcgc aggtcctgcg
     1141 cctgccgcgg gtgggggtga ccgacaactt cttcgagctc ggcggtgatt cgatcctctc
     1201 gatccagatc gtggcgcggg cgcgtcaggc cggtctgctg ttcaccgtgc gggagatctt
     1261 cgagcaccag accgtggccg agctagccct ccatgtcacg gtcgcggatg ccgcgggcgc
     1321 cgccctctcc gtccaggggc cggtgacggg tgaggtgccg ctcaccccga tccagagccg
     1381 gttcttcgat cagggcttgg cggacccaca ccacttcaac caggccctgt cgctggagcc
     1441 gcgggagcgc ctctctccca ccgctctgga ccgcgccatg gccgccatcg tggagcacca
     1501 cgacgccctg cggatgcggt tcgacggaaa gcggcaggag aacgcgccgg ccgagccggt
     1561 gacacctttc catcaggtgg acctctcggg cctgccggcg tctcgtcggg gcgaggccta
     1621 cgagtcggcg gccgcggctc ttcaggcagg cttcgagctg tccaccggac cactcacccg
     1681 cctctgtctc ttcgacgccg gggccggaca gccgtcacgg ttgctctggg tcacccatca
     1741 cctggtggtg gacggggtct cctggcgcgt gctgctcgaa gatttcgagg cggcgtaccg
     1801 ccaggccgcc ttgccgccca agaccacctc gttccaggag tgggctcgcc gtctgaccgg
     1861 gcatgccgag accctgatag gcgaactgga ttactggcgc gagactgccc gggttcccgt
     1921 gccccgtctg ccggtggact tcctgatgga agaagagaat ctcaacggag acgaggccac
     1981 ggtctccttc gagctcagct ccgaagagac ctctgatctg ctccagaccc tgccgtcggt
     2041 ctaccacagc cgcatcgacg acgcgctgct cagtgctctc gtccgggcgc tgaaggactg
     2101 gaccggctcc tcgcgcctgc gggtggacct ggaagggcac ggccgcgagc cgctcttcga
     2161 cgacctcgac gtctcgcgaa ccgtgggctg gtttacctcc atgtacccgg tcgtcctgga
     2221 ggcgggtgat gccgaccccg gcgaggcgct ggtgagcgtc aaggagcggc tgcgcgccgt
     2281 gcccgggcgc ggcatcggct atggcctgct gcggacggcc cgccttctgg aagaggcgcc
     2341 gccggccgag gttctgttca actatctggg ccaggccgac ggcaccgccg aggggctctc
     2401 cctcttccgg gcttccacga cctccaccgg cccttgccgg agcgtgcgcg cccgtcgtag
     2461 ccacctgttg gaggtcacag gcatcgtcac ggccggccgg ctccaggtca ccttgacgta
     2521 tgggtcccgg acgcatcgcc gggagaccgt ggagcggctg gccgcggcct atgccggcgc
     2581 gcttcgagag ctgatccagc acagccggga gagcgaagag gtcttcacgc cttccgactt
     2641 ccccaaggcc cgtctcgaca cccgcagctt tgacaaattg gcggctctgc tcgccgatga
     2701 cgactgaaga gcatcatgaa gaacgtcgaa gatatctata ccctcacgcc gctgcagagc
     2761 ggcatgttgt tccatagcct gatggcgccc gagtccggga tctacgtcaa ccaggtcacc
     2821 ggcaccctgc cggcggatct cgatgcccgg ctgttccagc aagcctggga gaggctcgtc
     2881 cagcgccatg gggtgctgcg caccgccttt ctctgggacg ggctcgaaga gccgctgcag
     2941 gtggtccgca agaaggtcgc tctcccctgg caggaactgg actggagtgg tctgcccagc
     3001 gaggagcagc agcgccgctt cgaagagctg cggcatcgcg aacggcacac gcctctgcct
     3061 ctgaccaagg cgccggtgat gcgcttctcg ctgatccgcc tcgatggcga gctggggttt
     3121 atctggacgt tccaccacct gctgctggac ggctggtcgc tccctctcct ggtccgggag
     3181 ctggtcgccg tctacacggc cctgcgggag ggccgggagc cgatgaagct actgccgccg
     3241 gcgcggccgt tcagcgacta catcgtctgg ttgcagaagc aggacgcgat caaggcggag
     3301 ccgttctggc gccgggatct cgcggggttt accgccccca atcctctcgg tatcgctcct
     3361 ccgggagccg agagcgctgc cggctacgcc gagcacgagc tccagctctc ccgtgaggtc
     3421 acggcggcgt tgcagactct ggcggcgcgg cacaagctga ccttgcagac tgtcaccctg
     3481 ggcgcctggg ccgtgctcct cagccgctac agcaacgagg aggacgtggt cttcggcaat
     3541 gtggtctccg gccgtccagc caccctgccc ggcgtcgaga ccatggtcgg catgttcgtc
     3601 aacaccctcc cggtgcgggt gcggttggac ggtgccgagc ccttggccac ttggctgcga
     3661 cgcctccagg agcgccagct cgcgcggcag gagttcgagc acaccccttt ggcccagatc
     3721 cagcgctgga gcgagattcc tgccggctcg ccgctgttcg agacgctcta cgtgttcgag
     3781 aactatccag acgccgacgg tggcggctcg gacgctctgc ggatcggcaa tctgcgcagt
     3841 tacgagagca ccaactatcc cgtcaccctg gccattatcg cggcggatca gctctctttg
     3901 cttctgaaga ccgaccgcgc acgcgtggat gaggatgcag cacaaggcct gctccagcac
     3961 ctggccacgc tgctcgccgg aatggcggag ggagcggacc gcccgatgac ggagctgcca
     4021 ctgctcaccg ccgcggagac cctccagctc tcggggtgga acgccacggc cagcgactat
     4081 ccccaggcct gcgttcatga gctgttcgcc gagcaggcgg agcgctctcc ggattccgtg
     4141 gctgtgacct tcggcggccg ggagctcacc tacggcgaga tcggccgccg ctcgcgtcag
     4201 ttggcgcacc ggctgcgccg agcgggcgtc gagcctggct ccctggtggg cctctgcgcc
     4261 gaatccttgc cggagctggt ggtagggatg ctgggcatcc tcgaggcggg aggcgcttac
     4321 gtgcctctcg acctcagcta tccccaggaa cggctcgcct tcatgttaga ggacaccggc
     4381 gccgccgtcc tcgtggcgga ggaggggctc gcggagcggc tccccatcca cgcagtcctg
     4441 cgcgttgagc ggctcggcca ttggccggcc gcggcggcaa gccgggagga gccggctttc
     4501 tcctcgcccg gcgatcccgc ctatgccatc tacacctccg gctcgaccgg ccggccgaaa
     4561 ggggtggtgg tgtcacaccg cgccgtcgtc cgcctcgtgc gcgggaccga ctacgtgcag
     4621 atcggtccgg atgatcgggt ggcgcaggct tcgaacagct cgttcgacgc cgccactttc
     4681 gagatctggg gcgcgctgct caacggtggc cgcttggtcg gcatcgagcg cgagaccgcc
     4741 ctttcgcccg agcgcctgac cggcgcgctt cgccgcgaag gcgtcagcgt gatgttcctg
     4801 accaccgccc tgttcaacca ggtcgcccgc gagctgccag ccgggttcgc gcatctgcgt
     4861 gccgtgctct tcgggggcga agcggtcgat cccgcggcgg tgcgcgcggt gttgcggggc
     4921 ggcccgcccc cgcgtctgct ccacgtctac ggcccgaccg aggcgacgac cttcgccaca
     4981 tggcagcggg tggaggaggt accgccgggt gaaaccgtgc ccatcggacg gccgctggcc
     5041 aacggcacct tgtacgtcat ggccgcggga atcgtgccgc agccagtcgg tctgccgggc
     5101 gagctctacc tgggcggcga cggtctggcg cacggctacc acgcccggcc ggagctgacc
     5161 gctgagcggt tcgtacccga tccgttcggc gcgctgactg gatccaccgc gggaggtcgg
     5221 ctctaccgga cgggcgacct tgtccggcag cggccggacg gagcggttga gttcctcggc
     5281 cgtctggacg gccaggtcaa gatccgtggc ttccgcattg agccgggaga ggtcgaagcg
     5341 gcactcctgg cgctgcccgg cgtccgcgaa gcggctgtag tggtgcggga ggacatgccc
     5401 ggcgagcggc gcctggtcgc ctatgcggct ggcgagctca ccgtcgagtc cctgcgtgcg
     5461 gccctacgcg agcggctgcc ggactacatg gcgccgtccg ccttcgtgcg cctcgacgcg
     5521 ctgcccctca accccaacgg caaggtggat cgaaaggccc tgcctgcgcc ggagcggtcc
     5581 gccggcgcgg agggctatct ggcgccgcgc acgccggtcg aggagatcct cgccggcatc
     5641 tgggccgagc tcctcgggct cgagcgggtg ggggcggacg gcaacttctt cgagttgggc
     5701 ggccattctc tccttgccac ccgggtgacg tcgcgcctgc gcgacaccct gggcatcgag
     5761 ctgccgctga gcgatgtctt cgaggcctcg agcctggcgg acctggccgc ccgcgtcgag
     5821 gcggcgcggc gagacggcac gcaggcatcc tccccgccaa tcgtaccggc gccgcgcgag
     5881 ggggccctgc ccctgtcatt cgcccaggaa cggctctggt tcattgatca gctcgagccg
     5941 ggaagcccgc tctacaacat cgcggcggcg ctacaggccc gcggcccgct ggactcgggc
     6001 gtactggcgc gcaccctcgg agagatcgtg cggcggcatg aagcactgcg cacggcattt
     6061 gccgagctgg ccggtgagcc ggtgcaggtg atccagccgg cccggccctt cctgctgccc
     6121 gtggtggatc tgtcagcgct gccggagagc gtgcgggagg acctggcgcg gaccctcgcc
     6181 gcggcggaga ccggccggcc cttcgatctc tcgcgcgggc cgctcatgcg cgctctgctg
     6241 ctgcgcctcg gcgaggcgga ccacgcggtg gtgctcacca tgcatcacat cgccagtgac
     6301 ggctggtcca tgggcatcct agtgcgcgag gtcgccgcgc tctatccggc cttcgccgcg
     6361 caccgcccgt cgcccctgcc cgagctgccg gtgcagtacg cggatttctc cgtctggcaa
     6421 cgctcctggc tgcacggcga ggtcctggag caggagatcg acttctggcg gcagcggctc
     6481 gccgggctgc cgccgctcct ggagctaccg accgaccacc cgcggcccgc ggtgcagagc
     6541 ttccgcggcg cggagcggac ggtgcgagtg ccggccgggc tcacccggcg gctggaggcc
     6601 ctagcccggc aggaaggggc aaccctcttc atggtgctcc ttgccggctt ccaggctctc
     6661 ctggcgcgcc atggcggaca ggacaatctc gccgtaggct cgcccgtagc cggacggaac
     6721 cggctcgaga ccgaaggact gatcggactc ttcgtcaaca cgctggtcct gcggggggat
     6781 ctcacaggtg cccccaagtt cagcgatctt cttggccggg tgcgcgagac cgccctcgcc
     6841 gcttatctgc atcaggacgt gccgttcgaa aagctggtcg aggagctcgc tccggagagg
     6901 agcctcgcgc acaccccgct gtttcaggtg atgttcacct tgcagagctt cgcgttccag
     6961 aggatcgatc tgcccgggct gtcgttttcg ccgctggcgg tcgagaccac gacggtgaag
     7021 ttcgacctgg aggtgaccgt cgtggagaca ggggatgggc tcagcgccca cttcgccttc
     7081 gcgagcgacc ttttcgatcc caccacgatc ctccgcctgt ccggtcatct gctgatgctc
     7141 ctggaaactg tggccgccga ccccgagcgg cgcccgggag agctgtccct gctgtccgaa
     7201 ggggagcacc atcagctcca gagcgaatgg aacgccgagc ccgccctggt ggcaccgggt
     7261 gtggtcgagc tgttcgcggc gcaggtgcgg cgcgcgccgg acgccgtggc cgtggctctc
     7321 gccgggagcg aggtgagctt cacctaccgc gagctcgatc gccgggcggg cctcctcgcc
     7381 gagggtctgc gcgctctggg ggtgggaccg gaggtgccgg tggggctctg catcgagcac
     7441 ggtccggagc tggcggcggc cgtgctcggg atcttccggt cgggtggcgc ttatgttccg
     7501 ctcgatcccg ctcaaccgcc ggcacggctg gccttcctgc tggaggattc ggcggtgccg
     7561 gttctggtta cccagacaca tttgctggcg ggcctgccgc cgcatggcgc ccaagtcgtc
     7621 ctggtgggag gagaagggcc ggtggaggag ggcggcgcct tgccgagccc cgctcctggc
     7681 gatctcgcct accggatcta cacctcgggg accaccggcc ggccgaaggc cgtgcaggtc
     7741 gagcacggca tgctcgcgtc taccctcgca gccacccgcc agcggttcgg gttcaccgcc
     7801 gacgaccgtg tgtcctgcct ggcgcagccc acgttcgaca tcttcctctt cgagctgctg
     7861 tcaccgctcc tgaacggcgg cacggccgtg ctcttcccca tgcgaccgag gctggacgcc
     7921 gagcaggtgg tcgaccacct gggggagctg accactctcc atgccgtgcc ggccttgatg
     7981 cggagcatcg tcgacgcgct ccaccgccgt ggccaggggg cgccggacgt gagcaagctg
     8041 cgccgggtct tcatcggtgg cgaggcggtg ccggccgacc tgctggagga gctgcggcag
     8101 gccttccctg gcgcccagat ctgtgtcctt tacgggccca ccgaggggac catcctctgc
     8161 tccgcgcacc cggtgccgcc gccccctgcc ccagccaggg cgctgctcgg ccggcccttg
     8221 cccggcgcgg tgctccacgt ccgcgacgcg gcgggagagc tgctgcccat cggtgtgccg
     8281 ggcgagctgt ggatcggcgg cccgggtgtc gcccgcggct acctgggccg ggaggagctg
     8341 acggcggaga agtacgtgct gcgggacggt cagcggttct accgcagcgg cgaccgc
//
LOCUS       DASPJI010000876         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_569611, whole genome shotgun sequence.
ACCESSION   DASPJI010000876 DASPJI010000000
VERSION     DASPJI010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569611"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1748
                     /locus_tag="VEL74_24780"
     CDS             <1..1748
                     /locus_tag="VEL74_24780"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HYG65819.1"
                     /translation="AWGLRNPFRLAFDPDAAGTRFFIGDVGQDAWEEIDEGRIGADYG
                     WNCFEGSRPRSTTGKCNPAPANTVAPLFEYEHRTDVPGTTSTNCNSVVGSAFVPTGVW
                     PGMDGVYLFGDYICGTIFTLTESGGAWSIGEFATGLRGIIHLEFGPHGDSQALYYTVY
                     TNGGQIRRIARQDTGNDPPTAALTASPLFGPVPLLVSFNGSGSSDPDPGDTLTYFWTF
                     GDGTPETSTTSPTAQHTYGSPGAYTARLRVRDSQMAFSSPVTVEIQADNSPPVPVITS
                     PSATAEFAVGQSVTLTGGATDPQDGTLPAGSLSWTVILHHNEHTHPFLGPVTGNGIVF
                     TAPPPEDLAATDSSYLEIQLTATDSHGLSATAIRDFQARKVDVTFETVPAGLTVTANG
                     TTLTGPETVTSWEGYELVVEAGTQVSGGEDWSFVSWSDGGAATHTIVTPASPATYTAT
                     FESAPSGGWSFYTLTPCRAVDTRRPAGTLGGPALTSGGTRTFEVAGTCGIPPTAAALA
                     VNVTVVSPTQSGHFRLYPAGGTVPGTSVINFSAGQVRANNAVLSLGTGSFSVLCGMAG
                     TGSAHLLVDVVGYFE"
     gene            complement(1798..2388)
                     /locus_tag="VEL74_24785"
     CDS             complement(1798..2388)
                     /locus_tag="VEL74_24785"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HYG65820.1"
                     /translation="MEPAQSPWPQPSQQPPSPPYAAAPPPPSTPAHAILSPPAGTGDP
                     LRRKSPKLAALLSVFPGLGNIYNGLYLRGFTFFAIFMGLIGMAEHEEVFVAAIMFFGL
                     FNIIDAYRQATLINYGYAQDLGILDMPEVPKAGQGGIIAGVVLTLIGLFALLERFFSV
                     NLEFLFDLWPAALMLIGLWLVVSSVRERLKDRDQAA"
BASE COUNT          389 a          881 c          819 g          358 t
ORIGIN      
        1 tcgcctgggg gctgcgcaac ccgttccggc tcgccttcga cccggacgct gccggcaccc
       61 ggttcttcat cggcgacgtg ggacaggacg cctgggagga gatcgacgag gggcggatcg
      121 gcgccgacta cggctggaac tgcttcgagg ggagccgccc ccggagcacg accggcaagt
      181 gcaacccggc gccggcgaat acggtcgccc ctctcttcga gtacgagcac cggacggacg
      241 tgccggggac gaccagcacc aattgcaact cggtggtggg cagcgccttc gtccccaccg
      301 gcgtctggcc cggcatggac ggcgtctacc tcttcggcga ctatatctgc ggcaccatct
      361 tcaccctcac cgagtcggga ggcgcctggt ccatcggcga gttcgccacc ggcctgcgcg
      421 gcatcatcca tctggagttc ggaccgcacg gagacagcca ggcgctttat tacacggttt
      481 acaccaacgg tgggcagatc cggcggatcg cccgccagga cacgggcaac gacccgccga
      541 cggcagccct gaccgcctcc cccctcttcg gccccgtccc cctgctggtg agcttcaacg
      601 gctcgggcag cagcgacccg gacccgggcg acacgctcac ctacttctgg accttcggcg
      661 acggcacgcc ggagacctcc accacctccc ctaccgccca gcacacctac ggatcgccgg
      721 gggcctacac cgcccggctg cgggtgcggg acagccagat ggccttctcc tcgccagtga
      781 ccgtggagat ccaggctgac aactcgccgc cggtgccggt gatcacctcg ccctcggcca
      841 cggcggagtt cgccgtcggg cagagcgtca ccttgaccgg cggcgccacc gatccccagg
      901 acggaactct gcccgcgggc tccctgtcct ggaccgtcat ccttcaccac aacgagcaca
      961 cccatccctt cctcgggccg gtgaccggaa acggcatcgt ctttaccgcg ccgcctcccg
     1021 aagacctggc cgccaccgac tccagctacc tggagatcca gctgaccgcc acggattccc
     1081 acgggctctc ggccacggcc atccgcgact tccaggcccg caaggtggac gtcaccttcg
     1141 agacggtgcc cgcgggcctg acggtcacgg ccaacggcac gaccctcacc ggccccgaga
     1201 ccgtgacctc ctgggagggc tatgagctgg tcgtggaggc ggggacccag gtgagcggcg
     1261 gcgaggattg gagcttcgtc tcgtggtccg acggcggcgc ggcgacccat acgatcgtca
     1321 cgccggcctc ccccgccacc tatacggcga cgttcgagtc cgccccatcc gggggatgga
     1381 gcttctacac tctgacgccc tgccgcgccg tggacacccg acggcccgcc gggaccctgg
     1441 gcggccccgc cctgacctcc ggcggcacgc ggaccttcga ggtggccgga acctgcggca
     1501 tcccgccaac cgccgcggcg ctggccgtca acgtcacggt ggtctccccc acccagtccg
     1561 gccatttccg gctgtacccc gcggggggca cggtgccggg gaccagcgtg atcaatttca
     1621 gcgctggcca ggtgcgggcc aacaacgccg tgctgtcgct ggggacggga agcttctcgg
     1681 tgctctgtgg catggccggc accgggtcgg cgcacctgct ggtggacgtg gtgggatact
     1741 tcgagtgagc cctcaccggc ccggtaaccg gtggcggcga gggctcactc gccggcctca
     1801 ggccgcctgg tcccggtcct tcaggcgctc ccgcacgctg ctcacgacga gccagagacc
     1861 gatcagcatg agggccgcgg gccagaggtc gaaaaggaac tcgaggttga cgctgaagaa
     1921 gcgctcgagc agggcgaaca ggccgatgag cgtcaggacg acaccggcga tgatcccgcc
     1981 ctggccggcc ttcggcacct cgggcatgtc gaggatcccc aggtcctggg catagccgta
     2041 gttgatcagc gtcgcctgcc gataggcgtc gatgatgttg aagaggccga agaacatgat
     2101 ggcggccacg aagacctctt catgttcggc catgccgatc aggcccatga agatggcgaa
     2161 gaaggtgaac cccctcagat acaggccgtt gtagatgttc cccagccccg ggaagaccga
     2221 aagcagggcc gccagcttcg ggctcttgcg ccggagcggg tcgccggtgc cggccggcgg
     2281 cgacaggatg gcgtgagccg gagtggacgg cgggggcggc gccgcggcat agggcggcga
     2341 aggcggctgc tgcgacggct ggggccaggg gctctgggcg ggttccattt ttatctcctt
     2401 ggtccggagc ccgaggccca cggaccggtc tcaatgatgt ccaggga
//
LOCUS       DASPJI010000877         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_569852, whole genome shotgun sequence.
ACCESSION   DASPJI010000877 DASPJI010000000
VERSION     DASPJI010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569852"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VEL74_24790"
     CDS             <1..367
                     /locus_tag="VEL74_24790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HYG65821.1"
                     /translation="EEHVEVTRGQLERLDRIFEQLGKSSRGKKCKGMEGLLEEGKEMM
                     EEDMEPEVLDAALLAAAQKVEHYEIAGYGTVRTYAQLLGEDQAAKLLQQTLDEEGETD
                     KRLTMLAEKNINVEAMAEA"
     gene            437..1303
                     /locus_tag="VEL74_24795"
     CDS             437..1303
                     /locus_tag="VEL74_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02587 family membrane protein"
                     /protein_id="HYG65822.1"
                     /translation="MARDGGRTGVSSPAQSLREYARGIAGGLLFSLPLLYTMEVWWAG
                     FIARPLNLLVYFAVGFLLLFGYNRYAGLHEDASWLDVAFEAVEEMGLGLLLATLLLWL
                     LGQIDSTQTLAEIVGKVVVEAVVVAIGISVGASQLGGNGKQGQGMRGDRGDEGPLSLP
                     AQLVLGACGAVLLASNVAPTEEIVMIAIESSRAKILALALFSYLLSCLVLFYSDFHGT
                     EDQVPPEGQGPVTALLRSAVNYSVALAASAFILWFFGRFSGMSLAVCVSQTVVLGVVA
                     SLGAAVGRLLLQ"
     gene            1300..1692
                     /locus_tag="VEL74_24800"
     CDS             1300..1692
                     /locus_tag="VEL74_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65823.1"
                     /translation="MSGLQDLRKNWLEWTVFAVGLVAVLGTFGYLAYEALAGEVSPPR
                     VIVELGRPTPDGSRWTVPVRVRNDGGETAEEVQVEVSLESPGHEPETAKLEVAFVPRK
                     SQREGWVTFSRDPAAGRLTGRAIGYEKP"
     gene            1770..>2697
                     /locus_tag="VEL74_24805"
     CDS             1770..>2697
                     /locus_tag="VEL74_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HYG65824.1"
                     /translation="MSLSHEDGNHNLTRLALGALGVVYGDIGTSPLYAMRECFYGTHS
                     VRPTPENVLGILSLIFWALIIIISIKYLVFVTRANNRGEGGILSLMSLIPAKTVGTGR
                     KRSWLLMTLGLFGAALLYGDGMLTPTVTVLSAVEGLEEATTFFNPYVVPITIVILVGV
                     FLLQRRGTAGIGAVFGPVMMLWFTTLAVLGVSWIWRHPEVLAAVNPAHAFSFFARNQG
                     MGFLALGSVFLVVTGGEALYADMGHFGRRPIQVAWFIMVLPCLLLNYFGQGALLLSGT
                     WTKGNPFYGLVPSWGLYPLVVLSTAAAIIASQA"
BASE COUNT          393 a          907 c          905 g          492 t
ORIGIN      
        1 cgaggagcac gtggaggtga cgcgcggcca gctcgagcgg ctcgaccgga tcttcgagca
       61 gctcggcaag agctcccgcg gcaagaagtg caagggcatg gaagggctgc tcgaagaggg
      121 caaggagatg atggaggagg acatggagcc cgaggtcctc gacgccgccc tcctcgccgc
      181 cgcccagaag gtggagcact acgagatcgc gggctacggc acggtgcgta cctatgccca
      241 gctccttggc gaggatcagg ccgccaagct cctccagcag accctcgacg aggaggggga
      301 gaccgacaag cggctcacca tgctcgccga gaagaacatc aacgtcgagg cgatggccga
      361 ggcctgagca gagtctcatc ccgccggcgg cgcgccgggc tcgcggaatg ggggtggtgg
      421 agggtaagat ggggccatgg cgcgcgacgg cggccggacc ggtgtcagct cgcctgccca
      481 gtcactccgt gagtacgccc gggggatcgc gggcgggctc ctcttcagcc tccccctcct
      541 ctacaccatg gaagtctggt gggccgggtt catcgcccgg cccctgaacc tcctcgtcta
      601 cttcgccgtc ggcttcctgc tgctcttcgg ctacaaccgc tatgccggcc tccacgagga
      661 cgcgagctgg ctcgatgtcg ccttcgaggc ggtcgaggag atggggctcg gcttgctgct
      721 cgccaccctc ctcctctggc tcctggggca gatcgactcc acgcagactc tggccgagat
      781 cgtgggcaag gtcgtggtcg aagccgtggt cgtcgccatc ggcatctcgg tgggcgcctc
      841 ccaactcggc ggcaacggca agcaggggca gggcatgcgg ggggaccgcg gcgacgaggg
      901 accgctcagc ctgccggccc agctcgtgct cggcgcctgc ggcgccgtcc tcctggccag
      961 caacgtcgcc cccaccgagg agatcgtcat gatcgccatc gagtcctcgc gcgccaagat
     1021 cctggcgctc gcgctgttct cctacctcct gagctgtctg gtccttttct atagcgactt
     1081 ccatggcacg gaggaccagg tcccgcccga aggacagggc cccgtgaccg ctctcctgcg
     1141 gagtgccgtc aactactcgg tggcgctggc ggcctcggcc ttcatcctct ggttcttcgg
     1201 acgcttttcc ggcatgtctc ttgcggtctg cgtctcccag acggtggtgc tgggggtggt
     1261 cgcctccctg ggggctgcgg tcgggaggct gctcctccaa tgagcgggct ccaggatctc
     1321 cggaagaact ggctggagtg gaccgtcttc gccgtcggtc tggtggcggt gctcggcacg
     1381 ttcggttatc tggcctacga ggccctggcc ggggaggtct ccccgccccg ggtcatcgtc
     1441 gagctcggcc ggcccacccc ggacggctcc cgctggaccg ttccggtgcg ggtccgcaac
     1501 gacggcggcg agacagccga ggaggttcag gtcgaggtga gcctggagag ccccgggcac
     1561 gagccggaga cggcgaagct cgaggtcgcc ttcgtgcccc ggaagtcgca gcgcgaaggg
     1621 tgggtcactt tctcccgcga ccccgccgcg ggccgcctca ccggcagggc gatcggctac
     1681 gagaagccgt gatagggtgg atagcttgtg aaacttggaa caaggacatc caccaggcct
     1741 gaaggcccgg atcttttcat aaagctccca tgtctctgag tcacgaagac ggaaaccata
     1801 atctcacccg cctcgccctg ggtgctctgg gcgtcgtcta cggagacatc ggcacgagcc
     1861 cgctctacgc gatgcgcgag tgcttctacg ggacgcacag cgtccggccg acgccggaga
     1921 acgtgctcgg cattctgtcg ctcatcttct gggcgctgat catcatcatc tccatcaagt
     1981 acctggtgtt cgtgacgcgg gccaacaacc gtggcgaggg gggcatcctg tccctcatgt
     2041 cgctgatccc ggccaagacg gtcggcacgg gacggaagag gtcctggctc ctcatgacgc
     2101 tcggcctgtt cggcgccgcc cttctctacg gcgatggcat gctcacgccg acggtcacgg
     2161 tgctctccgc cgtcgagggc ctggaggagg cgacgacctt cttcaatccc tacgtggttc
     2221 cgatcaccat cgtcattttg gtgggcgtct tcctcctgca gcgacggggg actgccggta
     2281 tcggcgcggt cttcggcccg gtgatgatgc tctggttcac caccctagcc gtgctcggtg
     2341 tgagctggat ctggcgtcat cctgaggtgc tcgccgccgt caatcccgcg cacgcgttct
     2401 ctttcttcgc ccgtaaccag ggaatgggtt tcctggcgct gggctccgtc tttctggtgg
     2461 tgaccggcgg tgaagcgctg tacgccgaca tggggcactt cgggcggcgt ccgatccagg
     2521 tggcctggtt catcatggtc ctgccctgcc tgctcctcaa ctacttcggc cagggggcgc
     2581 tcctgctgtc cggcacctgg acgaagggga acccgttcta cggcctcgtg ccgtcctggg
     2641 gcctctaccc gctggtggtc ctgtccaccg ccgccgccat catcgcctcc caggcgg
//
LOCUS       DASPJI010000878         4320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_570076, whole genome shotgun sequence.
ACCESSION   DASPJI010000878 DASPJI010000000
VERSION     DASPJI010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570076"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..674)
                     /locus_tag="VEL74_24810"
     CDS             complement(171..674)
                     /locus_tag="VEL74_24810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65825.1"
                     /translation="MTIKRSTATICLFLLLALPLIAQAQEKKTYTCASAESRRGTARM
                     GRGAGARNVQTVETEIEKTSRFLPCDVPDGVSIARFEGNARDGFDAVLAAINGELKKK
                     KQLTLEIDPAANDGLSKRQAKYLAGGYRVEDLLNSLCSGTCEWQIEEQGEARLLVVSP
                     LAAATAS"
     gene            complement(1300..2445)
                     /gene="rffA"
                     /locus_tag="VEL74_24815"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(1300..2445)
                     /gene="rffA"
                     /locus_tag="VEL74_24815"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HYG65826.1"
                     /translation="MPDRMMTDRIPFNRPALAGKEMEYIQDAVARGHLSGDGYYTRRC
                     HDLLEELLGVPRVLLTTSCTHALEMSALLLDLGPGDEVVVPAFTFVSTVNAFVLRGAT
                     PVFADIRPDTLNLDESRFAELVTPRTRAVVPVHYAGIACDMDPILETARAAGAVVVED
                     NAHGLFGAYRGRPLGTLGSLATLSFHETKNVACGEGGALLVNDPSLVARAEILREKGT
                     DRSRFFRGEVDRYTWVDVGSSHLPSDLLAAFLLAQLEQRDAIQERRRRIWERYQEGLA
                     SWAAARGARLPVVPPHCTQPYHLFYLLLPTTRERDALIEHLRELHIQAVFHYLPLHLS
                     PMGRCLGGREGQCPVTEDVSGRLVRLPFFPGITGEEQERVMEAVRGF"
     gene            complement(2445..>4320)
                     /locus_tag="VEL74_24820"
     CDS             complement(2445..>4320)
                     /locus_tag="VEL74_24820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65827.1"
                     /translation="RLAGREPGLRPRPAGAILASLIVRPPAPMSSTAEIQIPPRVRET
                     TRGDTLPHGRALLRDPAVALLLLVPLALLAIDPRWIYSPIHRDSWIYWGYFQNASTFA
                     WKFPDQYFSSRLSVILPGHLLHRLLPLEAANHVLHLTAFWAALLSFYGVCASWLDRRR
                     ALLAALALGCHPFFLNAIGWNYVDGFGITYFLATLFLLTAALRSRHRSLCLVLAGAGA
                     TALVTANAFYGIYLPFLAAYFLLFDRGPGRRPLLSSIALTLAGAAGLFLVFGTVNRIM
                     GGPFFYLAPSLSFIGGRVQTANPFRDPTYSWVPGATRLVLTLLILAGGAAVLALRRRE
                     ADPARRRFAVFTQLQLLGLAGLLVFCQVKADMAVLQHLYYVSLLLPAVFFAFAAQLPP
                     GLAGMKPRTFALLFGAAAGILGLSHALAFAPGSTVSWRPDLPVLAPLVAGGLLVVLPW
                     LNGLATKGAAAWTLGTLMSLAICQLLAWEPSNSGQGFSRFGGDTRGAFRQVAQASALI
                     DSRDPALGIRLWYTITPDDSNLLADMVSSTFLVCPSMVGGNFPALIFGAVMCDGTPLV
                     AGQRIAVLSTDPAAFDQADRSLRGVGLAARLLERQEIAGPVPGFAVTFLEAVPAAGAP
                     "
BASE COUNT          748 a         1399 c         1574 g          599 t
ORIGIN      
        1 gatctcccgc gaccgcggcg ccagggtgcg gacgccggaa ggctaccgcg ccctggccgc
       61 cggcttcgag gtgcaggagt ccggcgtgct caccgacctg atacggatcc cgtacagcca
      121 tttctatctg cgggcaacca ggctggccgc ccggagttga ggcctggggg tcaggacgcc
      181 gtcgcggcgg ccaggggaga aaccacgagc agccgcgcct ctccttgctc ttcgatctgc
      241 cactcacacg tgccactgca gagactgttg agcagatcct ccacccgata gcctccagca
      301 agatacttgg cctgccgttt ggagagcccg tcattcgccg ccgggtcgat ctcgagagtc
      361 aattgcttct tcttcttgag ctctccattg atggcagcga gaacggcgtc gaagccatcg
      421 cgggcattgc cctcgaatct ggcgatgctg acgccgtccg gcacatcaca ggggaggaag
      481 cgggacgttt tctcgatctc cgtctccacc gtctgcacat tcctggcacc agcccccctg
      541 cccatcctcg ccgtacccct acggctctcg gccgaggcac aggtgtatgt cttcttctcc
      601 tgcgcctggg cgatcagagg cagagcgagg agaagaaaga ggcagatcgt cgccgtcgaa
      661 cgcttgatcg tcatcttgga cctccataat cctgaggggg atcatgacga attttagcat
      721 ggtcccaaga tggcaaccgt tgtagcctat gaaaaggaaa gagcggggcg cctcgggaga
      781 ggcgcgcccg ctctacgggt ttggatggac gggcctacct acccgtggac ccgctcttcc
      841 aggatggcag aggacggagg ggcgatccct cagtcttgag ggaccgctcc tcaaggcttc
      901 gccgcggccc ctcaggactg agggacggct cctcagtgtt ccggaacggc tcctcagtac
      961 tgagggacag ttcctcagta ctgaggggga gctcctcagt gcttcgccgc cgttcctcaa
     1021 cgctacggag ccaccttgga gtgttacggc gcggtccctc aacgtcgagg ggctgtccct
     1081 caatggttcg ccgccgttcc tcaacactga gggatggctc ctcagtgctt cgccgccgct
     1141 cctcaacgct ccgccgcggc ccctcagtgc ttggccgcgg ttcctcagcg atgagggatc
     1201 gtccctcagt gttgaggcgc cattcctccc tgttgaggga tcgctcctca gtcttgagga
     1261 acggtggcga agtgttgagg agcggctcct caggccagcc tagaaccccc gcaccgcctc
     1321 catcacccgc tcctgctcct ccccggtgat ccccgggaag aaggggagcc gcaccagccg
     1381 gccgctgacg tcttcggtca ccgggcactg cccctcccgg ccgccgaggc agcggcccat
     1441 cggcgagagg tggaggggca ggtagtggaa cacggcctgg atgtgcagct cccggaggtg
     1501 ctcgatcagg gcgtcgcgct cccgggtggt ggggaggagg agatagaaga ggtggtaggg
     1561 ctgggtgcag tgcggcggca ccaccggcag gcgggctccc ctcgccgccg cccaggaggc
     1621 caggccctcc tgataccgct cccagatccg ccggcgccgc tcctggatgg cgtcccgctg
     1681 ctcgagctgg gcgaggagga aggcggccag gaggtccgag ggtagatggc tcgagcccac
     1741 gtccacccag gtgtagcggt ccacctcgcc acggaagaag cggctgcggt cggtcccttt
     1801 ctcgcgcagg atctccgccc gcgccaccag cgacgggtcg ttcaccagga gcgctccccc
     1861 ctcgccgcag gccacgttct tggtctcgtg gaagctcagc gtggcgagcg atcccagagt
     1921 gcccaggggc cggccgcggt aggcgccgaa gagaccgtgg gcgttgtcct ccaccaccac
     1981 ggctccggcg gcgcgggcgg tctccaggat cgggtccatg tcgcaggcga tgcccgcgta
     2041 gtgcacgggc accacggcgc gggtgcgcgg cgtgacgagc tccgcgaacc ggctctcgtc
     2101 caggttcagg gtgtccggcc ggatgtcggc gaagaccggc gtcgccccgc gcagcacgaa
     2161 ggcgttgacc gtggagacga aggtgaaggc ggggaccacg acctcgtcgc cggggccgag
     2221 gtccagcagc agcgccgaca tctccagagc atgggtgcag gaggtggtga ggaggacccg
     2281 cgggacgccc aggagctcct ccagcaggtc gtggcagcgc ctggtgtagt agccgtcccc
     2341 cgagagatgg ccgcgggcca cggcatcctg gatgtactcc atctccttcc cggccagggc
     2401 ggggcggttg aaggggatgc ggtccgtcat catccggtcg ggcatcaggg cgctccggcc
     2461 gcgggaacgg cttccaggaa ggtcacggca aagcccggga ccggccccgc gatctcctgg
     2521 cgctcgagga gacgggcggc cagccccacg ccgcgcaagg agcggtccgc ctggtcgaag
     2581 gcggcggggt cggtcgacag gacggcgatg cgctggccgg cgacgagggg cgtgccgtca
     2641 cacatcacgg cgccgaagat cagggcagga aagttgcccc ctaccatgct cgggcagacc
     2701 aggaaggtcg aggacaccat atcggcgagg agattggagt cgtccggggt gatggtgtac
     2761 cagaggcgga tgccgagcgc cgggtcccgg gaatcgatca gggcggaggc ctgggctact
     2821 tgccggaagg cgccccgggt atcgccgccg aagcgcgaga agccttgtcc gctgttcgac
     2881 ggctcccacg cgaggagctg gcagatggcg agcgacatca gggtcccgag ggtccacgcg
     2941 gcggccccct tggtagccag accgttgagc caggggagca ccaccagcag accgccggcc
     3001 accaggggag ccagcaccgg gagatccggc cgccatgaca cggtgctgcc gggagcgaac
     3061 gccagggcgt gggagaggcc gaggatgccg gccgcggccc cgaagagcag ggcaaaggtc
     3121 cgcggtttca tcccggccag cccggggggg agctgggccg cgaaggcgaa gaagaccgcg
     3181 ggcagcagca ggctcacgta gtagaggtgc tgaaggaccg ccatgtccgc cttcacctga
     3241 cagaagacca ggagcccggc gagccccagg agctggagct gcgtgaacac cgcgaaccgt
     3301 ctcctggccg gatcggcctc ccggcgccgc agggcgagca ccgcggcgcc cccggccagg
     3361 atcaggagcg tcagcaccag gcgcgtggcg cccggcaccc aggagtacgt ggggtcgcgg
     3421 aacgggttgg cggtctggac ccggccgccg atgaagctca acgacggcgc gagatagaag
     3481 aacggccccc ccatgatccg gttgaccgtg ccgaacacca ggaagagtcc cgccgccccc
     3541 gccagggtca gagcgatcga ggagaggagc ggccggcggc cggggccgcg gtcgaagagc
     3601 aggaagtagg ccgccaggaa cgggagatag atcccataga aggcgttggc tgtcaccagt
     3661 gccgtggccc cggcgccggc caggaccagg cagagcgacc ggtggcgcga gcggagcgcg
     3721 gccgtcagca ggaacagggt ggcgaggaag taggtgatcc cgaacccgtc cacgtagttc
     3781 cagccgatgg cgttcaggaa aaacgggtgg cagcccaagg cgagcgcggc gaggagcgcc
     3841 cggcggcggt cgagccagga ggcgcagacc ccataaaagg acagcagggc ggcccagaag
     3901 gcggtcaggt ggaggacgtg gttcgccgcc tccagcggca gcagccggtg gagcagatgg
     3961 cccggcagga tgaccgacag ccgcgaagag aagtactggt ccgggaactt ccaggcgaag
     4021 gtggaggcgt tctggaagta gccccagtag atccaggaat cccggtggat cgggctgtag
     4081 atccagcggg gatcgatggc caggagcgcc agcgggacga gcaggaggag ggccacggcg
     4141 ggatcgcgga gcagggcacg gccgtggggc agcgtgtccc cgcgcgtggt ctcacgcaca
     4201 cggggaggga tctggatctc ggcggtgctc gacatcggag cgggggggcg gacgatcagc
     4261 gaggcgagta tagcgcctgc cgggcgcggg cgaaggcccg gctctcgtcc cgccaggcgg
//
LOCUS       DASPJI010000879         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_857209, whole genome shotgun sequence.
ACCESSION   DASPJI010000879 DASPJI010000000
VERSION     DASPJI010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857209"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VEL74_24825"
     CDS             <1..872
                     /locus_tag="VEL74_24825"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HYG65828.1"
                     /translation="KLACDCVVVSDTSLYAPGQPSLIYGLKGLAYMEIRVTGPNRDLH
                     SGTFGGAVANPLNVLCQIVSQLRDPKTGKVLIPGFYDDVRPLEAWEREEFAKLPFDEA
                     AYSDELGVAELFGEEGYSTRERTWARPTCDVNGIFGGYEGKGAKTVLPSWGGAKVSMR
                     LVPDQKPEKIAELFTRYVQSIAPKSVKVEVEYLHGAKAVVVEAQGPIVEAAMDAMQEI
                     WGARPVRIREGGSIPIVAHFSEVLKAPVLLLGFGLADDGLHSPNEKFNISHYYKGIRS
                     VARLLDRLGEVRV"
     gene            869..1423
                     /locus_tag="VEL74_24830"
     CDS             869..1423
                     /locus_tag="VEL74_24830"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HYG65829.1"
                     /translation="MSSKDHLEKELKFPGVELEKLRERLAELEAERRGPGAFEDNWIL
                     DRGDELRASGRILRLRTDGSRARLTFKGPMSMEGNMKVREEREVEVENAEEARALFEN
                     LGYAVVRRYQKVREEWQLGGVTIALDHTPIGDFAEFEGERAEVVAKRCGFDPTKAERR
                     SYLRLYEDYLKTNPEAPPEMVFRQ"
     gene            1427..2515
                     /locus_tag="VEL74_24835"
     CDS             1427..2515
                     /locus_tag="VEL74_24835"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HYG65830.1"
                     /translation="MQGRRLRALVLAAGLGTRLRPLTESTPKPLLPVAGVPILGHTLA
                     QLAALGCEATAINLHYQGDKIRARLGDAFAGMPLTYSEEPEILGTLGALHPLRDFFAP
                     ADLVLLVNGDSLCRWPLRRMVRRHLAGQPQGTRATLLLTSRPDPAAFGGGVGIDREGW
                     ILSFRNGGPARQGEVARRHVFAGAHVISPELLARVGPGRADIVRDLYEPLLTPLDGAG
                     GARLAAVVTAGLWHDMGTPQRFLDGTLEWARASWPERLWRRSWISPEAGLAAGALVRQ
                     SSIEPGARVGEKARVERSILLSGARVGAGCVIRESIVGFGASVPPGTWVERRVITAQR
                     EGATPGLDDSVVGGAVYTPFAPAGEAGG"
BASE COUNT          358 a          857 c          950 g          363 t
ORIGIN      
        1 gcaagctcgc ctgcgactgc gtggtggtct cagacacctc cctctacgcg cccggccagc
       61 cgtcgctgat ctacggtttg aaggggctcg cctacatgga gatccgggtc accggtccga
      121 accgggacct gcactccggc accttcggcg gcgcggtcgc caacccgctg aacgtcctct
      181 gtcagatcgt ctcgcagctc cgcgacccga agaccggcaa ggtgctgatc cccggcttct
      241 acgacgacgt ccgcccgctg gaggcctggg agcgcgagga gttcgccaag ctcccgttcg
      301 acgaggccgc ctacagcgac gagctcggtg tggcggagct gttcggtgag gagggctaca
      361 gcacgcgcga gcgcacctgg gcgcggccga cctgtgacgt caacggcatc ttcggcggct
      421 acgaggggaa gggggcgaag accgtgctcc cgtcctgggg cggcgccaag gtgtcgatgc
      481 gcctggtgcc cgaccagaag ccggagaaga tcgccgagct cttcacccgc tacgtccagt
      541 cgatcgcgcc caagtcggtc aaggtggagg tggagtacct ccacggcgcc aaggcggtgg
      601 tggtcgaggc ccaggggccg atcgtcgagg cggcgatgga cgccatgcag gagatctggg
      661 gggcgcgccc ggtgcgtatt cgtgaggggg gctcgatccc catcgtcgct cacttctcgg
      721 aggtgttgaa ggcgccggtg ctgctgctgg gcttcggcct ggccgacgac ggcctgcact
      781 cgccgaacga gaagttcaat atcagtcact actacaaagg gatccgttcc gtggcacggc
      841 tgctggaccg gttgggcgaa gtcagggtat gagcagcaag gaccatctgg agaaagagct
      901 caaatttccg ggcgtcgagc tggagaagct gcgcgagcgg ctggccgagc tggaggcgga
      961 gcggcgggga ccgggcgcgt tcgaggacaa ctggatcctg gaccgcggcg acgagctgcg
     1021 ggcctcgggc cggatcctcc gcctgcgcac cgacggctct cgggcgcgcc tgaccttcaa
     1081 gggccccatg tccatggaag ggaacatgaa ggtgcgggag gagcgggagg tcgaggtcga
     1141 gaacgccgag gaggcccggg cgctgttcga gaacctgggc tacgccgtcg tccgccgcta
     1201 ccagaaggtg cgggaggagt ggcagctcgg cggcgtcacc atcgccctgg accacacccc
     1261 gatcggcgac tttgccgagt tcgaggggga gcgggcggag gtcgtggcca agcgctgcgg
     1321 cttcgatccg accaaggcgg agcgccgctc ctacctgcgg ctgtacgagg actatctcaa
     1381 gaccaacccc gaagcgcctc ccgagatggt gttccggcag taggccatgc agggtaggcg
     1441 actccgggct ctggtgctcg ccgccgggct cggcacgcgg ctgcgcccgc tcaccgagtc
     1501 cacaccgaag cccctgctgc ccgtggcggg ggtgccgatc ctgggtcaca ccctggcgca
     1561 gctcgccgcg ctggggtgtg aggccacggc catcaacctc cactaccagg gcgacaagat
     1621 ccgcgcgcgg ctcggcgacg ccttcgccgg catgcccctc acctattcgg aggagccgga
     1681 gatcctgggc acgctcggcg cgctccatcc gctgcgcgac ttcttcgcgc cggcggacct
     1741 cgtcctgctc gtcaacggcg acagcctctg ccgctggccg ctgcgccgga tggtccgccg
     1801 gcacctggcg ggccagccgc aggggacccg ggcgacgctc ctcctcacct cccggccgga
     1861 cccggccgcc ttcggcggcg gcgtgggcat cgaccgcgag ggctggatcc tgtcgttccg
     1921 gaacggcgga ccggcgcggc agggggaagt ggcgcggcgg cacgtcttcg ccggcgcgca
     1981 cgtgatctcg cccgagctcc tcgcccgggt cggaccgggc cgcgccgaca tcgtgcgcga
     2041 cctctacgag ccgctgctga ccccgctgga cggcgcgggc ggagcccggc tggccgcggt
     2101 ggtcacggcg gggctctggc acgacatggg aacgccgcag cggttcctcg acgggaccct
     2161 ggagtgggcg cgggcaagct ggccggagcg cctctggcgg cggtcgtgga tctcgcccga
     2221 ggctgggctg gcggcgggcg ccctggtgcg ccagtcgtcc atcgagccgg gcgcccgggt
     2281 cggtgagaag gcgcgggtgg agaggtcgat cctcctctcc ggggcccggg tgggcgccgg
     2341 ctgcgtgatc cgcgagtcga tcgtcggctt cggcgcctcg gtgccccccg gcacctgggt
     2401 cgagcgccgg gtgatcaccg cgcagcgcga aggggccacg ccgggcctcg acgactcggt
     2461 ggtgggcggc gccgtctaca cgccgttcgc tccggccggg gaggcgggag gatgaggccg
     2521 gcgcccta
//
LOCUS       DASPJI010000880         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_627367, whole genome shotgun sequence.
ACCESSION   DASPJI010000880 DASPJI010000000
VERSION     DASPJI010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627367"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VEL74_24840"
     CDS             complement(<1..535)
                     /locus_tag="VEL74_24840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65831.1"
                     /translation="MSRSALGPVLRFHLRVGVRIAMLILAPVLVGAVGGGVLLGDDFL
                     DSFSRMLYGSGSSGGSAVLIALACLGVASTAAPRICRGLDGWLRHLPVDGLTHRRAAA
                     VAIAVSQTPLLAFLLFLSLFATRITAPSVIDALALPVTALGAALWAVPAERGVLTKIL
                     GLAGAIAAGSGGWRWLAA"
     gene            complement(532..1620)
                     /locus_tag="VEL74_24845"
     CDS             complement(532..1620)
                     /locus_tag="VEL74_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006971069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HYG65832.1"
                     /translation="MTAALDGARPVRTGEELPPAFLEAWLRGRLPDLPDSPLEVEQFP
                     GGHSNLTYLLRLGDRELVLRRPPAGSKVKTAHDMGREHRVLSRLADLYPKAPRALAHC
                     DDPSVIGAPFYVMERVRGVILRGSRPPKGVDLTPERMRLLSTAAVDGLAELHAVDFRA
                     AGLGDLGRPEGYVERQVRGWTERWVASRTDDVPDIDRAAAWLADHLPPGLTPTALLHN
                     DYKYDNLVLSGDLSEIVAVLDWEMATVGDPLMDLGTSLGYWIDADDEPAMHALALGPT
                     ALPGNLTRAEVVERYAARSGRDVSGILFHYVYGLLKVAVIVQQIYYRYKQGLTRDERF
                     AAFLEVVRVLGRTAALAIGRGRIDRLHG"
     gene            complement(1617..>2597)
                     /locus_tag="VEL74_24850"
     CDS             complement(1617..>2597)
                     /locus_tag="VEL74_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HYG65833.1"
                     /translation="LAEVLGRSPLGHYVFNCQAPDAGNMELLLHHGTPEQRERWLGPL
                     ARGEVRSCFGMTEPDFPGSNPVWMGTTARRDGDDYVLDGRKWFTSSADGAAFCIVMAV
                     TDPEAPPHARASQILVPLETPGFGVVRNLSVMGEPGEGWMSHAEVRLDGVRVPVANRV
                     GAEGAGFTLAQERLGPGRIHHCMRWLGICERAFDLLCGRAATRELAPGDVLGNRQLVQ
                     AWIAESRAEIDAAKLLVLRTAWRIDREGAPAARDDISLIKFHTAGVLQAVLDRAIQAH
                     GALGLIDETPLAFWWRHERAARIYDGADEVHKTTVARRILKGYAPVEDEP"
BASE COUNT          377 a          961 c          950 g          309 t
ORIGIN      
        1 ccgccgccag ccacctccac ccacccgacc ctgcggcgat ggcccccgcc agccccagga
       61 tcttggtcag caccccccgc tccgccggca ccgcccagag cgccgctccg agcgccgtga
      121 ccggcagggc cagcgcgtcg atgaccgagg gcgccgtgat ccgggtcgcg aagagggaga
      181 ggaagagcag gaaggcgagg agcggcgtct gcgacacggc gatggcgacc gccgcggcgc
      241 gccggtgcgt gaggccgtcc accggcagat ggcgcagcca gccgtccagg ccgcggcaga
      301 tccgcggcgc cgcggtcgag gccaccccaa ggcaggcgag ggcgatgagc accgccgagc
      361 cccctgagga gcccgagccg taaagcatcc gggagaacga atcgaggaag tcgtcgccga
      421 ggagcacgcc gccgcccacc gcgccgacca gcaccggcgc caggatcagc atggcgatcc
      481 gcacccccac ccgcaggtgg aaccggagga cggggccgag cgcggaacgg ctcatccgtg
      541 cagccggtcg atccggcccc ggccgatagc gagcgccgcg gtccggccca gcacccggac
      601 gacctcgagg aacgccgcga agcgctcgtc ccgggtcagc ccctgcttgt agcgatagta
      661 gatctgctgg acgatcaccg cgaccttgag cagcccgtag acgtagtgga agaggatgcc
      721 ggagacgtcc cggccgctgc gggcggcgta gcgctccacc acctcggcgc gggtgaggtt
      781 ccccggcagc gccgtgggcc ccagcgccag ggcgtgcatc gccggctcgt cgtccgcgtc
      841 gatccaatag ccgaggctcg tccccaggtc catcagcgga tcgccgaccg tggccatctc
      901 ccagtcgagc accgccacga tttcggagag atcgcccgag agcaccaggt tgtcgtactt
      961 gtagtcgttg tggaggagcg ccgtcggcgt caggccgggc gggaggtggt ccgccagcca
     1021 ggccgcggcg cggtcgatgt ccggcacgtc gtcggtgcgg gaggcgaccc agcgctcggt
     1081 ccagccgcgc acctggcgct cgacgtaccc ctcgggccgc ccgagatcgc ccagcccggc
     1141 ggcccggaag tccaccgcgt gcagctcggc caggccgtcc accgccgccg tggagaggag
     1201 acgcatccgt tccggcgtca ggtccacccc cttcggcggc cgcgatcccc gcaggatgac
     1261 cccgcgcacc cgctccatca catagaacgg cgcgccgatc accgatgggt cgtcgcagtg
     1321 cgccagcgcc cgcggggcct tggggtagag gtcggcgagg cgcgacagca cccggtgctc
     1381 ccggcccatg tcgtgcgccg tcttcacctt cgagccggcg ggcggccggc ggagcaccag
     1441 ctcgcggtcc cccaggcgca gcagataggt gaggttcgag tggccgccgg ggaactgctc
     1501 gacttcgagt ggagagtccg gtagatcggg cagccgcccg cgcagccagg cttcgaggaa
     1561 ggccggcggc agctcctccc cggtccgcac cggccgcgcc ccgtcgagcg cggcggtcaa
     1621 ggctcgtcct ccaccggggc atagcccttc aggatgcgcc gcgccaccgt ggtcttgtgg
     1681 acctcgtcgg cgccgtcgta gatccgcgcc gcccgctcgt ggcgccacca gaaggcgaga
     1741 ggggtctcgt cgatgagccc gagggcgccg tgcgcctgga tcgcccggtc gagcacggcc
     1801 tggaggacgc cggcggtgtg gaacttgatc agcgagatgt cgtcgcgggc ggccggggct
     1861 ccctcgcggt cgatccgcca cgcggtacgg aggacgagca gcttggcggc gtcgatctcg
     1921 gcccggctct cggcgatcca ggcctggacg agctggcgat tccccaggac gtcgccgggg
     1981 gccagctcgc gggtcgccgc ccggccgcag agcaggtcga aggcgcgctc gcagatgccg
     2041 agccagcgca tgcagtggtg gatacggccc gggccgagcc gctcctgggc cagggtgaac
     2101 cccgccccct cggcgccgac gcggttcgcc accggcaccc ggaccccgtc gaggcggacc
     2161 tcggcgtggc tcatccaccc ctcgccgggc tcgcccatca cggagaggtt gcggacaacc
     2221 ccgaagccgg gcgtctccag ggggacgagg atctggctcg cccgggcgtg cggcggggct
     2281 tcggggtcgg tgacggccat gacgatgcag aaggcggcgc cgtcggcgga cgaggtgaac
     2341 cacttgcggc cgtcgaggac gtagtcgtcc ccgtcacggc gcgccgtggt ccccatccag
     2401 accgggttgg agcccggaaa gtccggctcg gtcatgccga agcagctccg gacctccccc
     2461 cgcgccaggg gtccgagcca gcgctcccgc tgctccggcg tgccatggtg caggaggagc
     2521 tccatgttgc cggcgtccgg cgcctggcag ttgaagacgt agtggccgag cgggctccgc
     2581 cccagcacct cggcgag
//
LOCUS       DASPJI010000881         2845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_627870, whole genome shotgun sequence.
ACCESSION   DASPJI010000881 DASPJI010000000
VERSION     DASPJI010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627870"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VEL74_24855"
     CDS             complement(<1..701)
                     /locus_tag="VEL74_24855"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HYG65834.1"
                     /translation="MRQDCTTATLFLTGMQRSGTTLLDKLLAGHPRLSVLSQPFPLLF
                     VEAKRSFLRTLGERDASYPLGDLFLETRYRPRDLAAHLAAQRLDGTALAPLFAVMESF
                     SGQYTRFAPARTAEVLAGLEPGDLAAVAAQIYRALAHRPGAILTGGKETTCEELLPYL
                     LDRGYRCLLVLRDPRDVLASLAHGEGRRHGGRLKPTLFNLRQWRKSVAFALHLEDHPG
                     CLWLRYEDLAARPAET"
     gene            733..1509
                     /locus_tag="VEL74_24860"
     CDS             733..1509
                     /locus_tag="VEL74_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011650551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HYG65835.1"
                     /translation="MKRVAVLQSSYIPWKGYFDLINLVDEFILYDDVQYTRRDWRNRN
                     LVKTSGGTAWMTIPVQVKGRYHQAIRAVEVADPRWASRHWKSLQIHYGRARSYAAVAP
                     ALSRLYELAAGERLLSGVNEVFLRGICGLLGIRTVITHSSDYELREGRTDRLAGLCEQ
                     AGAAEYLSGPSARAYLDEGLFRSRGIAVRWMSYDGYPEYRQLFSPPFVHQVSILDLLF
                     NEGVEGARHHMLSTRSATAPAVVPASSAPPSPELLACSSP"
     gene            1494..2417
                     /locus_tag="VEL74_24865"
     CDS             1494..2417
                     /locus_tag="VEL74_24865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HYG65836.1"
                     /translation="MLVSVVVPVYGSTVLAELAGRVHAVFAARPADDYELIFVDDGSP
                     SPEVWSVLERLATGEGRVTALQLTRNFGQQPATLCGLREARGEVVITMDDDLQHRPED
                     IPALLDAQTAGGWDIVIGQLVERRHPWPRRLASRIKGYFDHLLIGKPRHIQLTSFRLL
                     TRTVVDGVLSIRTPNPFLPAMMFHVTKSVGGVPVAHQARRQGRSGYSLRKLLRLFSNL
                     VINNSSLLLRLVGQVGIVCAILSVLGACLVVYRKLAHGIAIEGWASLFTAMLFIGGLL
                     LFGLGVVGEYLVRIIESSEAKPTYFVRRKAG"
     gene            2414..>2845
                     /locus_tag="VEL74_24870"
     CDS             2414..>2845
                     /locus_tag="VEL74_24870"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG65837.1"
                     /translation="MTAGTPAHRSRGLYRLLEPAAAYELLQRLVGAGPARGRFVQEIL
                     RPRPGDRLLDIGCGAGGLLDALPDHVAYVGYDLNPRYIESARRRHGARGEFHQARAGE
                     PPAEMLAGTFDLVVAKSILHHLDDREAGELMEVARRALKPGG"
BASE COUNT          418 a          965 c         1026 g          436 t
ORIGIN      
        1 gtctcggccg gccgtgccgc caggtcttcg tagcgcagcc agaggcagcc ggggtgatcc
       61 tcgaggtgca gagcgaaggc cacactcttg cgccactgcc gcaggttgaa gagggtgggt
      121 ttgagccggc cgccgtgccg ccgcccttcg ccatgggcga gcgacgccag cacgtcgcgt
      181 gggtcccgca ggacgaggag gcaccggtag ccgcggtcca ggaggtacgg gaggagctcc
      241 tcgcaggtcg tctccttgcc accggtcagg atcgcgccgg ggcggtgggc gagggcgcgg
      301 tagatttgcg ccgcgaccgc ggccaggtca cccggctcga ggcctgccag gacctcggcg
      361 gtccgcgcgg gggcgaaacg ggtgtactgg ccggagaagc tttccatgac cgcgaagaga
      421 ggtgccaggg ccgtgccatc gagccgctgc gccgccaggt gggccgccag atcccgcggc
      481 cggtaccgcg tctccaggaa caggtcgccc aggggatagg aagcgtcccg ttctccgaga
      541 gtccggagga acgaccgctt ggcctcgacg aagaggaggg ggaagggctg cgacagcacc
      601 gacaggcgcg gatggcctgc caggagcttg tcgagcaggg tggtgcccga gcgctgcatc
      661 ccggtgagga agagcgtcgc cgttgtacaa tcctgtcgca caaagccttt ctgtgtcccg
      721 gcgaatccac ccatgaagcg agtcgcggtc cttcagtcct cgtatatccc ctggaaaggg
      781 tatttcgacc tgatcaatct ggtcgacgag tttatcctct acgacgacgt ccagtacacg
      841 cgccgcgact ggcggaaccg gaacctcgtg aagacgagcg gcgggaccgc ctggatgacg
      901 attcctgtgc aggtgaaggg gagataccac caggcgatcc gcgcagtcga ggtcgccgac
      961 ccgcgctggg cctcgcggca ctggaagtcc ctccagatcc actatgggcg ggcgcggagc
     1021 tacgccgcgg tggctccggc gctgagccgg ctctacgagc tggcggccgg cgaacggctc
     1081 ctctccgggg tcaacgaggt gttcctgcgc gggatctgcg gcctgctggg gatccgcacc
     1141 gtcatcaccc actcctcgga ctacgagttg cgggaggggc gcaccgaccg cctggcgggg
     1201 ctctgcgagc aggcgggggc ggccgagtac ctctccgggc cgtcggcccg cgcctatctg
     1261 gacgaagggc tgttccggtc gcgggggatc gccgtccgct ggatgagcta cgacggctac
     1321 cccgagtacc ggcagctctt cagcccgccg ttcgtccacc aggtgagcat cctggacctg
     1381 ctgttcaacg agggggtgga gggggcgcgg catcacatgc tgagcacgcg gagcgccacg
     1441 gcacccgccg tggtccccgc ctcctcggcg ccgccttcgc cggagctcct cgcgtgctcg
     1501 tctccgtagt cgtcccggtc tacggcagca cggtcctggc ggagctggcc gggcgggtcc
     1561 atgccgtctt cgccgcccgt ccggcggacg actacgagct gatcttcgtc gacgacggct
     1621 cgccgagccc ggaggtctgg tcggtcctgg agcgtctcgc caccggggag gggagggtga
     1681 cggccctcca gctcacccgc aacttcggcc agcaacccgc caccctctgc ggtctgcgcg
     1741 aggcgcgggg agaggtcgtg atcaccatgg acgacgacct ccagcaccgg ccggaggaca
     1801 tcccggcgct cctggacgcc cagactgcgg gcggctggga catcgtcatc ggccagctcg
     1861 tggagaggcg tcatccgtgg ccgcgccggc tggcgagccg catcaagggc tacttcgacc
     1921 acctcctcat cggcaagccg cggcacatcc agctcacctc cttccggctg ctgacccgga
     1981 cagtggtcga cggcgtgctg tcgatccgca cccccaaccc gttcctgccg gcgatgatgt
     2041 tccacgtcac caagagcgtg ggaggggtgc cggtggcgca tcaggcgcgg cgtcagggga
     2101 ggagcggcta ctcgctaagg aagctcctgc ggctcttcag caacctggtg atcaacaact
     2161 ccagcctgct gctgcgcctg gtcggacagg tggggatcgt ctgcgccatc ctgagcgtcc
     2221 tgggggcctg tctggtggtc taccgtaagc tggcccacgg gatcgccatc gaaggctggg
     2281 cctcgctgtt caccgcgatg ctcttcatcg gcggtctcct gctcttcggg ctgggagtgg
     2341 tgggggagta cctggtgcgg atcatcgaga gcagcgaggc gaagcctacc tacttcgtcc
     2401 gccggaaggc cggatgaccg ccgggacgcc agcgcaccgt tcgcgcggcc tctaccggct
     2461 gctcgagccc gcggccgcct atgagctgct ccagcgcctg gtgggggccg ggccggcaag
     2521 gggccgcttc gtccaggaga tcctgcggcc acgccccggc gaccggctcc tcgacatcgg
     2581 ctgcggcgcc ggggggctcc tggacgcgct gccggatcac gtcgcctacg tgggctacga
     2641 cctgaatccg cgctacatcg aatccgcccg gcggcgtcac ggcgcccggg gggagttcca
     2701 ccaggcgagg gccggagagc cgccggcgga gatgctggcc ggcaccttcg acctcgtcgt
     2761 cgccaagtcg atcctgcatc acctggacga ccgggaggcg ggggagttga tggaggtcgc
     2821 gcgccgggcg ctcaagccgg gcggc
//
LOCUS       DASPJI010000882         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_280992, whole genome shotgun sequence.
ACCESSION   DASPJI010000882 DASPJI010000000
VERSION     DASPJI010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280992"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /locus_tag="VEL74_24875"
     CDS             complement(<1..1605)
                     /locus_tag="VEL74_24875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65838.1"
                     /translation="MLTIEPPFYQVRGVTVFRDHADPDQFWVLPPPPRLVQDEDGPAF
                     TLYKYRRDLTDNPALDPTRARGAGLALFEVEAALEKQVLLQAEVAGLSGRPDARLSPV
                     LFRSGTVRAILAHAEGDSFVEDLAESRIAPIASPHRAAFSLALSAEGATLFEQAARGG
                     DIPAGVVYELRFLALTPALSARVRMDYDRMYDRFSASVGFTYYVSAKLDLDLAWLVEH
                     DIIKVEILSFTDDADMARQREMVMDLVKVRVQQDFFRSGLPPKGQEAAGGALAEMLAG
                     LVGAGEVTSASALFVLKAKLEIVREHKDFELLYDGRAAVELTHVATGLLPVMVTEETP
                     LDVREIDLDDPFFSALDVQVVSAIDFAEMADLREAVVHLSVPLAGGEHRASFRVAADE
                     PGPFRFQAPLTERGKDEYTFQVEYHFDPALGKGEPRVAAGPFRSRHRVLVIDPLAHFR
                     YRRLRLLAGPVDPARVPRLRVRVRLLGEEGADDLARETFELDARTPEAVWRQHLPMAF
                     STPPLRARTDWQDPRGDWHEGEEMDAE"
     gene            complement(1628..>3060)
                     /locus_tag="VEL74_24880"
     CDS             complement(1628..>3060)
                     /locus_tag="VEL74_24880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65839.1"
                     /translation="YVLEELFEPSLGQAPPAEDPDDDALATIGRAIKDVAGFFSLTYS
                     LRTLDRSELKTFTYQLAASRAERLTLAPQGTLRALLPEGTAADRLIVEVEPGPAAEMR
                     FDIGTAVDLAAEGIDHLEVRLLYGDKEEDVLLDAAAPRKQASLWHRPELGPDVAWRYE
                     AHLLPGAGGLEGRLTSPERTAAGRVIRIDPRELYQRVEVRAVAQGLPFDRWPAVLVDL
                     RATDPVAGWTATETLRLDASRPEAAFQVRAGLGAPVRFERRLRYLDPRGDETTVDWEE
                     TEAGVLLVGDPLPEVVDVQILGSARFGTDVRRLILELRPKSDPSRVATRVLTAEQPSA
                     TWSWAAPEGADRSYEYRVTVHTARNEVREGPWLPGPSGKLVVGEGIARLRQVQLLLVG
                     KSPRDLGLLALKVRFAFEDVESGLFAEDERLVDDARAPIRWDYPVADPARQAYSYQLT
                     LVQGDGTMDVRPPVTTSDLLVVQPLA"
BASE COUNT          434 a         1126 c         1105 g          395 t
ORIGIN      
        1 ctcggcgtcc atctcctccc cctcgtgcca gtccccccgc gggtcctgcc agtcggtgcg
       61 cgctctcagg ggcggagtgg agaaggccat gggaagatgc tggcgccaga ccgcctcggg
      121 cgtccgggcg tccagctcga aggtctccct cgccaggtcg tccgccccct cctcgccgag
      181 cagccgcacc cggacccgca gccgggggac ccgggcgggg tcgaccggac cggcgagcag
      241 gcgcaggcgg cggtaccgga agtgggcgag ggggtcgatc accagcaccc ggtgccgcga
      301 ccggaacggc ccggcggcca cccgcggctc gccctttccc agggccgggt cgaagtggta
      361 ctccacctga aacgtgtact cgtccttgcc gcgctcggtg agcggcgcct ggaagcggaa
      421 cgggccgggc tcgtccgctg ccacccggaa ggaggcgcgg tgctcgccgc ccgccagggg
      481 cacggacaga tggacgaccg cctcccggag gtccgccatc tccgcgaagt cgatcgccga
      541 gaccacctgg acgtccagcg cggagaagaa ggggtcgtcg aggtcgatct cccgcacgtc
      601 gagcggcgtc tcctcggtga ccatgacggg cagcaggccg gtggcgacgt gggtcagctc
      661 gacggcggcg cggccgtcgt agagcagctc gaagtccttg tgctcgcgga cgatctccag
      721 cttcgccttg agcacgaaga gcgccgacgc cgacgtcacc tccccggcgc ccaccagccc
      781 ggccagcatc tcggcaaggg cgccccccgc cgcttcctgc cccttggggg ggagccccga
      841 ccggaagaag tcctgctgca cgcggacctt caccaggtcc atcaccatct cgcgctgccg
      901 cgccatgtcc gcgtcgtcgg tgaaggagag gatctcgacc ttgatgatgt cgtgctcgac
      961 cagccaggcg aggtcgaggt cgagcttggc cgagacgtag taggtgaagc ccaccgaggc
     1021 cgagaagcgg tcgtacatcc ggtcgtagtc catgcgcacc cgggcggaca gggccggggt
     1081 gagggccagg aagcgcagct cgtagaccac gccggccggg atgtcgccgc cgcgggcggc
     1141 ctgctcgaag agcgtcgccc cctcggcgga gagcgccagg gagaaggcgg cgcggtgcgg
     1201 ggaggcgatc ggggcgatgc ggctctcggc caggtcctcg acgaacgaat ccccctccgc
     1261 gtgcgccagg atggcccgca ccgtgccgga cctgaagagg acgggggaga gccgcgcgtc
     1321 cggccgcccc gacagtcccg ccacctcggc ctggagcagc acctgcttct cgagcgccgc
     1381 ctccacctcg aacagcgcga ggccggcgcc gcgcgcccga gtcgggtcga gcgccgggtt
     1441 gtccgtcagg tcgcgccggt atttatagag ggtgaacgcc gggccgtcct catcctggac
     1501 cagccgcggc gggggaggga ggacccagaa ctggtccggg tcggcgtggt cccggaacac
     1561 ggtgacgccg cgcacctggt agaagggggg ctcgatggtc agcatgatgg gtctccgtcg
     1621 atctccttca cgccagcggc tgtaccacca gcaggtccga cgtggtcacc ggcggccgca
     1681 cgtccatcgt gccgtcgccc tgcaccaggg tgagctggta gctgtaggcc tgccgcgccg
     1741 ggtcggcgac ggggtagtcc cagcggatgg gggcgcgggc gtcgtcgacc agccgctcgt
     1801 cctcggcgaa gaggccagac tccacgtctt cgaaggcgaa gcgcaccttg agcgccagga
     1861 gccccaggtc gcgcggggac ttgccgacca gcaggagctg cacctgccgc agccgggcga
     1921 tcccttcgcc gaccaccagc tttccggacg gacctggtag ccacggcccc tcccgcacct
     1981 cgttgcgcgc cgtgtgcacc gtcacccggt actcgtagga gcggtccgcc ccctcgggcg
     2041 ccgcccacga ccaggtggcg gacggctgct cggcggtcag cacccgcgtc gccacccgcg
     2101 acgggtcgga cttcgggcgc agctccagga tcagccgccg cacgtcggtg ccgaagcgcg
     2161 ccgagccgag gatctggacg tccaccacct cgggcagggg atcacccacc agcagcacgc
     2221 cggcctcggt ctcttcccag tccaccgttg tctcgtcccc tcgcgggtcg agatagcgca
     2281 ggcgccgctc gaagcgcacc ggcgcgccga gcccggcgcg cacctggaag gccgcctcgg
     2341 gccgggaggc gtcgagccgt agcgtctccg tcgccgtcca tccggccacg ggatcggtcg
     2401 cccggagatc caccagcacc gccggccagc ggtcgaaggg gaggccctgc gccacggccc
     2461 tcacctccac ccgctggtac agctcgcgcg ggtcgatccg gatcacccgc ccggccgccg
     2521 tccgctccgg ggacgtcaac cgcccctcca agccgccggc gccggggagc agatgggcct
     2581 cgtagcgcca ggcgacgtcc ggccccagct ccggccggtg ccagagggag gcctgcttgc
     2641 ggggcgcggc ggcgtcgaga agaacgtcct cctccttgtc gccgtagagc agccgcacct
     2701 ccaggtgatc gatcccctcg gcggcgaggt ccacggcggt gccgatgtcg aaccgcatct
     2761 ccgccgccgg gccgggctcc acctcgacga tcagacggtc ggccgccgtc ccctcgggga
     2821 ggagcgcgcg cagcgtccct tgcggcgcga gcgtcagccg ctcggcgcgg gaggcggcga
     2881 gctggtaggt gaaggtcttg agctcactgc ggtcgagggt gcgcaggctg taggtcaggg
     2941 agaagaagcc ggccacgtcc ttgatcgccc ggccgatggt ggccagggcg tcgtcgtccg
     3001 ggtcctcggc cggcggcgcc tgcccgagac tgggctcgaa cagctcctcg agcacgtagc
//
LOCUS       DASPJI010000883         8458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_168935, whole genome shotgun sequence.
ACCESSION   DASPJI010000883 DASPJI010000000
VERSION     DASPJI010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8458)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8458
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168935"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VEL74_24885"
     CDS             complement(<1..824)
                     /locus_tag="VEL74_24885"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HYG65840.1"
                     /translation="MPDEIETLLRRAAGDRDVIAMGGGLPSPRQFPRRRLVEALADVV
                     LQPAPSALQYSWPEGDEALRRRIADYLASRDAAVEPERILVTSGAQQALMIATRLHCP
                     EGARIYTDPESYPGALELFRSRGLTPIPLDGAPDPEAACAVYVMPAVSNPRGETMDEE
                     TRRGLLASGLPILEDDAYGDLLFSGPGPRPLLAEAPDRVFYIGTFSKTLAPGLRVGWL
                     VVPERLHEEALKAKRDADLQANGLGQAIVERYLRADGYEERLKRLRTFYRRRAKRLA"
     gene            complement(920..1582)
                     /locus_tag="VEL74_24890"
     CDS             complement(920..1582)
                     /locus_tag="VEL74_24890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65841.1"
                     /translation="MPPEVRAREAFGDLLPEVFSGEVLVKADYNNVVVALDEQDAEGR
                     TDGRVDHLFLFVTLSPLIPPFTLSDAAAEIEYRPTGLRVLSHTKQRELELQINGAPPT
                     PRPKQSVYSFTASRSGIHLYRYSGAAVKQLSLQDVSGEEAQLLTTLRDKSALLGPDSG
                     SELGEFTPDPTAGSGSSSCNPSCSKSCAFGSCSAACIPGHCAKCECLGDPPTLFPSCF
                     CA"
     gene            complement(1762..4953)
                     /locus_tag="VEL74_24895"
     CDS             complement(1762..4953)
                     /locus_tag="VEL74_24895"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HYG65842.1"
                     /translation="MAIETRSLTSPAPPDTTKILQGYLQRVFELERREDVREESFYSS
                     LEELLVRCAGHLNRGDVRVTVIPKRREGHLLDFQVWRGGRPVGYVEAKRPGTDLQRVE
                     ESPQLERYKAAFPNLILTNFHQFRLYREGERVARADIASPFAARLLPRELMAGAARVE
                     GEPALLALLDGFFTFEAPWSGSASSLAVAMAGRTRELAARMEEILEREEGRDGKGTFT
                     GLHQAFAEYLLGRLSRREFADLYAQTVAYGMLAARWRSPEGFDRATLFDRIPRTNGIL
                     RDVFRYISLGDPPPEVRWILDDLAHLLAPAPVRGLLERHYHAGRGKDPILHFYETFLA
                     SYDRDLRRRRGVFYTPQPVVAYVVRSVHRLLADRLGLDGGLAHPGVCLLDPAAGTLTF
                     VAEALREAAAAHRERFGDGAVPALVRDRLLPNFHAFELMMAPYAIGHLHLGLVLAELG
                     CPLEEEDRFGLYLTDALDARELKQAALPGLSSLARESHLAGRLKKEVPEGCSAFVVVG
                     NPPWLGRSSQRSSEVRDGYLAPDGRRDEGYYRLDGQPLGEANPRWLQDDYVQFIRFAQ
                     RRIDEVGQGVLGFVTNHGYLDNPTFRGMRASLLGTFDELYVLDLHGNMKKKERSPDGS
                     RDENVFEGVRQGVAIALFVKKPGLPRRVWRADLHGSHAEKLRWLSANDVASTPWRELR
                     PEAPAYLFAPCDVDLEKEYLRGTPLPKIFPRHSAGVVTARDAFAIDRDPRELEARIGN
                     WCTPTNRGPFTDLRNTGTWSLEKARRRALADEDWRTRFQKILYRPFDERHVFYADYLL
                     ERPRENVMRHMLAGGNLGLVAPRQSKDGAGALVTDRIVAHKAVSAYDINYLFPLWLLP
                     EEEDGLFKGETRKPNLAPDLLRSLDRLYGEEPEPEEVLGYVYAVLYSPTYRERYAPLL
                     RQDFPRIPFPWDRHLFEELAALGTELIGLHLLRTVPACPSATFQGKGNGTTGRAGFPR
                     YDPAERQVVINECGQCFQGIEPEVWDYRIGGYQVLRRWLQARAGLRLLDDEIRSFCRT
                     VAVLEETIRLQRGIDALYVAVGDSQALEACSGP"
     gene            5160..6875
                     /locus_tag="VEL74_24900"
     CDS             5160..6875
                     /locus_tag="VEL74_24900"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYG65843.1"
                     /translation="MRRELSGPGRTRAWSAFALTLILTAAAVAPAVAAADSPDVAAID
                     RYLTAAYAANEPGAAVLVKRGDEVLLRKGYGMANLELGVPIQPDMTFELGSLTKQFTS
                     AAILMLAEQGKLSVEDEVTRYLPDFPTHGRKITLEHLLTHTSGIPSYTGLPDWMAKAR
                     QDMSVDELIGVFKGRPLEFEPGEKWAYNNSGYVLLGAILEKLSGKTYEQFVEEEIFKP
                     LGMTSSRYGSLSEVIPGRVSGYGRTPDGNWSNAPYLSRTLPYAAGALVSTLDDLARWD
                     RALSAGDGEGKLLSRASLERIYTPYRLASGKSSRYGYGWFVSELQGQRSLEHSGGIFG
                     FVTYMLRVPEERLLVVVLSNHPGKSPDPEEVAMRVAARALGRPFEDTPTVKLAAAALD
                     EYTGVYRIDEAAARMVTREGDRLFAHRTGSRKLEVVATARDEFFYPDSYTRLRFVRDA
                     RGKVAGMEVTPRGGETEAAQRTAEPLPAPRQAVRVDPAVFAGYIGKYQLAPGFVLDVT
                     REGEQLFVKPTGQTKVRLLPSSETEFFVEQADARFVFTRGADGKATELTLHQAGRQAP
                     AARVP"
     gene            6925..>8458
                     /locus_tag="VEL74_24905"
     CDS             6925..>8458
                     /locus_tag="VEL74_24905"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HYG65844.1"
                     /translation="MRLRTSLALALWLWAAIVMAAPAVSPETVAGIDRFLTETYPAAE
                     PGAAVLVMKDGEVLLRKGYGMANLELGVPITPDMVFELGSVSKPFTATAILMLAERGK
                     LSIEDDVTRHLPGFPTHGRKVTLAHLLSHTSGVPNFTSLPDWVGKQRLDLTVDELIGL
                     FRGRPLDFEPGERYSYSNSGYVLLGAVIEKISGRTYEEFIEEEIFKPLGMTASRYGRA
                     AEVIPGRVDGYEPDPKSGPHEKRWRNSPYLSMNIPYAAGALLSTLDDMARWDRAVTEG
                     TLLPRAALERMVTPFRLNSGKPARYGLGWTLSELAGHEVVEHNGATLGFMSHMLRVPD
                     ERLLVLVMSNYPAKTPRVETVALRVAARALGRPFEEIPTLKMTAAELEDYVGAYRVDP
                     KEVRMVTRDGDRLFVQRIGVRRAEVLPTAKDEFLFTDNYSRLRFLRDAQGRVTAMEVF
                     PRGGPSEVVPRSGEPPPGARPVVKVDPALFDAYAGTYQLAPGSLLLVSREGDRLFVQL
                     TGQ"
BASE COUNT         1323 a         2877 c         3015 g         1243 t
ORIGIN      
        1 gccagccgct tggcgcggcg gcgatagaag gtgcggaggc gcttcagccg ctcctcgtag
       61 ccgtccgctc tcagatagcg ctccacgatc gcctgcccca ggccgttggc ctggaggtcg
      121 gcgtcgcgct tggccttcaa agcctcctcg tgaagccgct ccggcacgac gagccagccg
      181 acgcgcaggc cgggggccag ggtcttcgag aacgtaccta tatagaagac gcgatcgggg
      241 gcctccgcca ggaggggacg ggggccgggg ccggagaaga ggaggtcgcc gtaggcgtcg
      301 tcctcgagga tcggcaggcc gctggcgagc aggccgcgcc gggtctcctc gtccatggtc
      361 tcgccgcgcg ggttggagac ggcgggcatg acgtagacgg cacaggccgc ctcgggatcg
      421 ggcgcgccgt ccagagggat cggggtgagg ccccgggacc ggaagagctc cagcgcgccg
      481 gggtagctct cggggtccgt atagatccgc gcgccctcgg ggcagtggag gcgcgtggcg
      541 atcatcaggg cctgctgcgc gccgctggtc accaggatgc gttccggctc gacggccgcg
      601 tcgcgggagg cgagatagtc cgcgatccgc cggcggagcg cctcgtcccc ctcgggccag
      661 gagtactgga gcgccgacgg agcgggttga agcacaacgt cggccagcgc ctcgaccagc
      721 cggcggcggg ggaactggcg cggcgacggc aacccgccac ccatggcgat cacgtcgcgg
      781 tcaccggcgg ctcgccgcaa cagggtttcg atctcgtccg gcatgccggt gtcgccgaag
      841 caagtcccgt gccccgggcg ggatgccgga cagacggatc agccgaggcc agccatgtgg
      901 ccctgctcgt tctggttcct caagcgcaga agcagctcgg gaacagagtc gggggatcgc
      961 cgaggcattc gcacttcgcg cagtgcccag gaatgcaagc ggccgagcag gagccgaacg
     1021 cgcaggactt gctgcaggaa gggttacaac tgctgctgcc ggaccccgca gtcggatccg
     1081 gagtgaactc cccgagctcg gagcccgaat ccgggcccaa gagagcgctc ttgtcgcgga
     1141 gggtggtaag aagctgggct tcttctcctg agacgtcctg cagtgagagc tgcttcaccg
     1201 cggcccctga atagcggtaa agatggatcc cgctccggga ggccgtgaag gagtacaccg
     1261 actgcttggg ccgaggagta gggggagccc cgttgatttg gagctcgagc tcccgctgct
     1321 tggtatgaga caggacgcgc aggccggtcg ggcgatattc gatctcggcc gcggcgtcgc
     1381 tgagagtgaa aggcggaatc aatggcgaca gcgtcacgaa gagaaacagg tgatccacgc
     1441 gaccgtcggt ccgtccttcg gcgtcctgct catcgagagc gacgacgaca ttgttgtagt
     1501 ccgccttgac gaggacttcg ccggagaaca cctcgggcag cagatccccg aatgcttccc
     1561 tcgcgcgtac ctccggaggc actccctgct cctgtgagcc cggcgtgctc ccgggaggag
     1621 ctccggctgc atgagcagcg ccggcaagac cgagagacag gagaacgcct acgagaattt
     1681 tacatcccca tcttggccac atcagcagat cctctctctg ttgagaaatc aatcgcatcg
     1741 tagtaggcgc tgctggatag gtcaagggcc ggagcaggcc tccagggctt ggctgtcgcc
     1801 gacggccacg tagagcgcat caatcccccg ctgcagccgg atcgtctcct ccagcaccgc
     1861 caccgtccgg caaaaactgc ggatctcgtc gtcgagtagc ctgaggccgg cgcgggcctg
     1921 gagccagcgg cggaggacct ggtagccgcc gatccggtaa tcccagacct ccggctcgat
     1981 cccctggaag cactgcccgc actcgttgat caccacttgt cgctcggcgg gatcgtagcg
     2041 ggggaagccg gctcttcccg tggtgccgtt tccctttcct tgaaaggtgg ccgacgggca
     2101 ggccgggacg gtgcgcagca ggtggaggcc gatcagctcg gttccgagcg cggccagctc
     2161 ctcgaagagg tggcggtccc aggggaaggg gatgcgcggg aagtcctggc ggagcagcgg
     2221 cgcgtacctc tcccggtagg tggggctgta gagcacggca tacacgtagc ccagcacctc
     2281 ctcgggctcc ggctcctctc catacaggcg gtccagagag cggagaaggt ccggcgccag
     2341 gttgggcttc ctcgtttccc ccttgaacag gccgtcttcc tcttcaggaa ggagccagag
     2401 ggggaacagg tagttgatgt cataggcgct cacggccttg tgggcgacga tccggtcggt
     2461 caccaaggct ccggcgccgt ccttggattg gcgcggcgcc accagtccca gattgcctcc
     2521 ggccagcatg tgacgcatca cattctcgcg gggccgctcc agcaggtagt ccgcgtagaa
     2581 gacgtggcgc tcgtcgaagg gccggtagag gatcttctgg aagcgggtgc gccagtcctc
     2641 gtcggcgagc gcgcgccggc gcgccttctc gaggctccag gtccccgtat tccgcaggtc
     2701 cgtaaacggt cctcggttgg taggtgtaca ccagttgccg atccgtgcct ccagctcccg
     2761 ggggtcgcgg tcgatggcga aggcgtcgcg cgccgtcacc acgccggccg agtgccgcgg
     2821 gaagatcttg gggagcgggg tgcctcgcag gtactccttc tcgaggtcca cgtcgcacgg
     2881 cgcgaacagg taggccgggg cctcgggccg cagctcccgc cagggcgtcg acgcgacgtc
     2941 gttcgcggac agccagcgga gcttctccgc gtgcgagccg tggaggtcgg cccgccagac
     3001 gcggcggggg agacccggtt tcttgacgaa gagcgcgatc gccaccccct gccgtacccc
     3061 ttcgaagacg ttctcgtccc gcgagccgtc cggcgacctt tccttcttct tcatgttgcc
     3121 gtggaggtcc aggacgtaca gctcgtcgaa ggtcccgagg agcgaggccc gcatcccccg
     3181 gaaggtgggg ttgtccaggt agccgtggtt ggtgacgaag ccgagcaccc cctggcccac
     3241 ctcgtcgatc cgccgctggg cgaagcggat gaattggacg tagtcgtcct gcagccagcg
     3301 cgggttggcc tcgccgagcg gctggccgtc caaccggtaa tagccctcgt cccgccggcc
     3361 gtcgggggcg aggtagccgt cccgcacctc ggagctgcgc tgggaggagc gcccgagcca
     3421 gggcgggttg cccaccacga cgaaggccga gcacccctcc ggcacctctt tcttgaggcg
     3481 ccccgccagg tgggactcgc gggcgaggga cgacagaccc ggcagggcgg cctgcttgag
     3541 ctcccgcgcg tccagcgcgt ccgtcaggta gagcccgaag cggtcttcct cttctaaggg
     3601 acagccgagc tcggcgagga cgaggccgag gtggaggtgg ccgatggcgt acggggccat
     3661 catcagctcg aaagcgtgga agttggggag cagccggtcg cggaccaggg cggggacggc
     3721 gccgtcgccg aagcgctccc ggtgagcggc cgccgcctcg cgcagcgcct cggccacgaa
     3781 ggtcagcgtg ccggcggccg gatcgaggag gcagacgccg gggtgggcga gcccgccgtc
     3841 caggccgaga cggtccgcga gcagccggtg gacggagcgg acgacatagg cgaccacggg
     3901 ctgaggggtg tagaagacgc cgcggcgccg gcgcaggtcc cggtcgtagc tggcgaggaa
     3961 cgtctcgtag aagtggagga tgggatcctt gcccctcccg gcgtggtagt ggcgctccag
     4021 gagcccgcgc accggcgcgg gggcgagcag gtgggcgagg tcgtcgagga tccaccgcac
     4081 ctcggggggc ggatcgccga gagagatgta ccggaagaca tcgcgcagga tgccgttggt
     4141 gcgcgggatg cggtcaaaca gcgtcgcccg gtcgaagcct tcgggggagc gccagcgggc
     4201 cgccagcatg ccgtaggcga cggtctgggc atagaggtcg gcgaactcgc ggcgcgacag
     4261 cctccccagg aggtactcgg cgaacgcctg gtggagcccg gtgaaggtcc ccttcccgtc
     4321 ccggccctcc tcccgctcca ggatctcctc catgcgggcg gccagctccc gcgtccgccc
     4381 ggccatcgcc accgcgagcg acgaggcgct gccgctccag ggcgcctcga aggtgaagaa
     4441 gccatcgagc agcgccagca gcgcgggctc cccctcgacg cgggcggcgc cggccatcag
     4501 ctcgcggggg agcagccgcg ccgcgaacgg ggaggcaatg tccgcgcggg cgaccctctc
     4561 cccttcccga tagagccgga actgatggaa gttggtgagg atcaggttgg ggaaggccgc
     4621 cttgtagcgc tcgagctgcg gcgactcctc cacccgctgg aggtccgtcc ccgggcgctt
     4681 ggcctccacg tagccgaccg gacggccgcc gcgccagacc tggaagtcga ggaggtgccc
     4741 ctcccggcgc ttggggatga ccgtcacccg cacgtcgccg cgattcaggt gtccggcgca
     4801 gcgcacgagc agctcctcga gagatgaata gaagctctcc tcccggacgt cctcccgccg
     4861 ctccagctcg aagacccgtt gcagatagcc ctgcaggatc ttcgtcgtgt cgggcggcgc
     4921 cggagaggtg agggagcgtg tctcgattgc catgcgaagt atccgatagc gaatacatca
     4981 tagcgcatcg agttgccgtt atgcgcaccg ccaggcttgg gtgaagcggg ggagatcgcc
     5041 gggaggcgcg acgggcgggc tctggtccac ggagaaatag ttcctccggg ccggcgtact
     5101 aagagcgacg gaggcggacc caccgggcgg ccgccccata caactcagga gcttccttga
     5161 tgagacgaga gctttccggc ccgggccgca cgcgtgcgtg gagcgccttc gccttgactt
     5221 tgatcctgac cgccgcggca gtggcacctg ccgtggcggc cgccgattct cccgatgtcg
     5281 ccgccatcga tcgctacctg accgcggcct atgccgccaa cgagcccggc gccgccgtcc
     5341 tggtgaagcg gggcgacgag gtgctgctgc gcaagggcta cggcatggcc aacctggagc
     5401 tgggggtgcc catccagccg gacatgacgt tcgagctggg ctccctcacc aagcagttca
     5461 ccagcgcggc catcctgatg ctcgcggagc agggcaagct ctccgtcgag gacgaggtca
     5521 cccgctacct ccccgacttc ccgacccacg gcaggaagat caccctcgag cacctgctca
     5581 cccacacctc gggcatcccc agctacaccg gcctgccgga ctggatggcc aaggcgcgcc
     5641 aggacatgag cgtcgacgag ctcatcggcg tgttcaaggg caggccactg gagttcgagc
     5701 cgggagagaa gtgggcctac aacaactcgg ggtacgtcct gctcggcgcc atcctcgaga
     5761 agctctcggg caagacctat gagcagttcg tcgaggagga gatcttcaag cccctgggca
     5821 tgacctcctc gcgctacggc agcctgtcgg aggtgatccc cgggcgcgtc tcgggctacg
     5881 gccggacccc cgacggcaac tggagcaacg ccccctacct cagccggacc ctcccgtacg
     5941 ccgccggcgc cctggtctcg acgctggacg acctggcgcg ctgggaccgc gcgctttcgg
     6001 ctggagacgg ggagggcaag ctgctgtcca gggcctccct ggagcggatc tacacgccct
     6061 accggctggc ttcggggaag tcgagccggt acggctacgg ctggttcgtc agcgagctcc
     6121 agggacagcg gtcgctcgag cacagcggcg gcatcttcgg cttcgtcacc tatatgctgc
     6181 gcgtcccgga ggagcggctg ctggtggtgg tcctgtccaa ccacccgggc aagtcgccgg
     6241 accccgagga ggtggcgatg cgggtcgcgg cgcgggccct cggccgtccc ttcgaggaca
     6301 cccccaccgt caagctggcc gccgcggcgc tggacgagta caccggcgtc taccggatcg
     6361 acgaggccgc ggcccggatg gtgacccgcg agggggaccg cctgttcgcc caccgcaccg
     6421 gcagccgaaa gctcgaggtg gtggccaccg cccgcgacga gttcttctat ccggacagct
     6481 acacccggct gcgcttcgtg cgcgacgccc gggggaaggt cgcgggcatg gaggtgactc
     6541 cgcgcggcgg cgagaccgag gcggcgcagc ggaccgccga gccgctgccg gcgccgcgcc
     6601 aggcggtgcg ggtggacccg gccgtcttcg ccggctacat cggcaagtac cagctcgcgc
     6661 ccggcttcgt gctcgacgtc acccgggagg gggagcagct cttcgtcaag cccaccgggc
     6721 agaccaaggt gcggctcctc ccctcctcgg agaccgagtt cttcgtcgag caggcggacg
     6781 cccggttcgt cttcacccgc ggcgccgacg gcaaggcgac cgagttgacg ctccaccaag
     6841 ccgggcggca ggcgccggcg gcgcgggtgc cctagctgga ggaggtgcga tcgacggcgg
     6901 cgggtcgtca tatagttgac gcccatgcgc ctccggacct ctctcgccct cgccctgtgg
     6961 ctgtgggcgg ccatcgtgat ggccgccccg gccgtgtccc cagagaccgt cgccggcatc
     7021 gaccggttcc tcaccgagac ctatcccgcg gccgagcccg gcgccgccgt cctcgtgatg
     7081 aaagacggcg aggtgctgct gcgcaagggc tacggcatgg ccaacctgga gctcggggtg
     7141 ccgatcacgc cggacatggt gttcgagctg ggctcggtct ccaagccctt caccgccacg
     7201 gccatcctga tgctcgccga acggggcaag ctctcgatcg aggacgacgt cacacgccac
     7261 ctgcccggct tcccgaccca cggcaggaag gtcaccctgg cgcacctgct cagccatacc
     7321 tccggtgtgc ccaacttcac ctccctgccg gactgggtcg gcaagcagcg cctggacctg
     7381 accgtcgacg agctgatcgg cctgttccgc ggcaggcccc tggacttcga gccgggggag
     7441 cgctactcgt acagcaactc gggctacgtg ctgctgggcg cggtcatcga gaagatctcg
     7501 ggccgcacct acgaggagtt catcgaggag gagatcttca agcccctggg gatgaccgcg
     7561 tcgcgctacg gcagggcggc ggaggtgatc cccggccggg tcgacggcta cgagcccgat
     7621 cccaagagcg gcccgcatga gaagaggtgg cgcaattccc cctacctcag catgaacatc
     7681 ccctacgccg ccggcgccct gctctcgacg ctggacgaca tggcgcgctg ggaccgcgcg
     7741 gtcacggaag ggacgctgct gcccagggcc gccctggagc ggatggtcac cccgttccgg
     7801 ctcaattcgg gcaagcccgc gcgctacggc ctcggctgga cgctcagcga gctcgccggc
     7861 cacgaggtgg tggagcacaa cggcgccacc ctcggcttca tgagccacat gctgcgcgtc
     7921 ccggacgagc ggctgctggt gctggtgatg tcgaactacc cggcgaagac gccgcgggtg
     7981 gagacggtgg ccctgcgggt cgcggcgcgg gccctcggcc ggccgttcga ggagatcccc
     8041 accctgaaga tgacggccgc ggagctggag gactacgtcg gcgcctaccg ggtcgacccg
     8101 aaggaggtgc ggatggtcac ccgcgacggc gaccgcctct tcgtccagcg gatcggcgtc
     8161 cggcgcgccg aggtgctgcc gacggccaag gacgagtttc tcttcaccga caactactcg
     8221 cggctgcgct tcctccgcga tgcccagggg cgggtcacgg ccatggaggt gttcccgcgc
     8281 ggcggcccgt ccgaggtggt gccgcggagc ggcgagccgc cgccgggagc gcggccggtg
     8341 gtcaaggtcg acccggcgct cttcgacgcc tacgccggga cctatcagct tgcccccggc
     8401 tcgctgctgc tggtgagccg cgagggggac cgcctgttcg tccagctcac ggggcaga
//
LOCUS       DASPJI010000884        11702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_169562, whole genome shotgun sequence.
ACCESSION   DASPJI010000884 DASPJI010000000
VERSION     DASPJI010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 11702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11702)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11702
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169562"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1162
                     /locus_tag="VEL74_24910"
     CDS             275..1162
                     /locus_tag="VEL74_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYG65845.1"
                     /translation="MIELAKSRLPEILGRHEREILEEWMQQQLAAATNRRDLIQEREL
                     AEQSRDFLAAFRSALTQDGAGDVDGPAWGGVREILAELSRSRARKGFSSSETATFVFS
                     LKQPLFERLRQHLGQDGAALGEAIWSLTTLLDNLGLWTTEVYQKGREEVIHRQQQELL
                     ELSTPVVRLWTNILALPLIGTLDSARTQVVMENLLQEIVNAGAAIAIIDITGVPTVDT
                     LVAQHLLKTVAAARLMGADCIISGIRPQIAQTIVHLGVELGDVTTKASLADAFALALR
                     RTGLKVTTAQPMMQPAAGA"
     gene            1175..1564
                     /locus_tag="VEL74_24915"
     CDS             1175..1564
                     /locus_tag="VEL74_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015205498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HYG65846.1"
                     /translation="MDRIPILKMGEFLLVTIQVDMHDRLVMTLQEDLMEQIRRTEARG
                     VLIDISALDVVDSFIGRMLGHIAAMARVLDAQTVVTGMRPAVAITLVELGLSLKGVRT
                     ALDVERGMDLLRTSVEEQENVRGSLAT"
     gene            1545..1949
                     /locus_tag="VEL74_24920"
     CDS             1545..1949
                     /locus_tag="VEL74_24920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HYG65847.1"
                     /translation="MAVSRPEELPIQREEDVVRVRQKVREWAVDLGFGLVDQTKVVTA
                     ASELARNTMLHGGGGRVLLEMVTEGTRRGLRLTFEDQGPGIPDLDLALKDGFTTGSGM
                     GLGLGGSKRLMNEFSVSSVVGAGTRVTVVKWK"
     gene            1946..3019
                     /locus_tag="VEL74_24925"
     CDS             1946..3019
                     /locus_tag="VEL74_24925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding SpoIIE family protein phosphatase"
                     /protein_id="HYG65848.1"
                     /translation="MSLPRMWDGEVIDFAVPVTEVSQVGEARRLISTVTRRLRFDETA
                     AGKAAIVATEMAGNLVKHGGGGEIIVRPLAHGERQALEILALDRGPGIADVAKSLRDG
                     YSTAGTPGTGLGAVARLSDGFDLYSAPGQGTALLSRLWSRAAPPGGTDGFHEPAEHGA
                     VCLPVTGEEAPGDSWEIQQRPGGWRLLVADGLGHGPFARDPAQRAIGAFRETPAGSPA
                     EVLEACHAALHGTRGAAVAVADVDLEAGTVRFAGVGNVAATILGEGPSLYMVSLNGIV
                     GQGAARFREFSYPWSPGALLVMASDGLSTRWKLDSYPGLAARHPSLIAGVLYRDHGRP
                     RDDITVVAVRAAQLRRGAGGGAR"
     gene            3016..5184
                     /locus_tag="VEL74_24930"
     CDS             3016..5184
                     /locus_tag="VEL74_24930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG65849.1"
                     /translation="MRPALLTVDIRQEADVVVARQRSRQLARLLGFDAQDQVRIATAV
                     SEIARNVYQYAGRGRIEFQLEGRTPPQVFVARLTDQGPGIADVPLILSGRYRSQTGMG
                     LGITGARRLMDQLQIESAPGKGTTVWLKKLLPAGAPYLDGPRLAAVGDALAREGFQNP
                     LAEVQNQNQELLRALGELRNRQDELLRVNRELEDTNRGVVALYAELDEKAEHLRRADE
                     MKSRFLSNMSHEFRTPLNSILALSRLLEERTDGELTGEQAKQVGFIKKAAGDLTELVN
                     DLLDLAKVEAGKTVVRPIEFEARNLFGALRGMLRPLLVAESVNLVFEDQADLAPLYTD
                     EAKVSQILRNFISNALKFTERGEIRVSATLSHDLDGGVEGVCFAVADTGIGIAPEDHE
                     RIFREFDQVEGRLQAKFKGTGLGLALSKRLAELLGGRITLESTLGAGSTFSLTIPRVY
                     ESDEPVEEEPLAEEWHPDPRRLPVLIVDDSPESLHVYERLLRDTPFQPVSTRTLREAA
                     ARLRTVHPAAIVLDIQLVGEQTWSFLAGLKGADETRDIPVLVITSVDDPAKAAALAAD
                     GYCDKPVDRDWLLERLGSLTRKAGKALIIDDEEASRYILKGLVVRLPLEPVESSDGRE
                     GLRRAREERPDVILLDLVMPGLTGFDVLDELRADPSTREIPVVVSTSKILAQEERQRL
                     ERWSVPVLSKETWARPDAVTELRQALSRAGWSSGSAEERA"
     gene            5181..6782
                     /locus_tag="VEL74_24935"
     CDS             5181..6782
                     /locus_tag="VEL74_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HYG65850.1"
                     /translation="MKPQPVILHVEDNEAVRYGIGRVLLREGFVLQEAPTGEEGLRRA
                     RESPDLILLDVKLPDMSGFDVCKLLKSDPETFSIPVLHLTSTYGSSEHLAAGLEGGAD
                     GYLTHPVEPIVLVATIRALLRARVAERQVQELLRLEAAARAAAEQANRLKDEFLATLS
                     HELRTPLNAILGWVQLLRSGSMDTAGVARAVETIERNAKAQAQLIADILEVSRIITGK
                     LRLDVHPLDLRSVVRSAAETVRPGAEAKGVALDLSLDRDVPPVSGDPDRLQQVVWNLL
                     SNAIKFTPRGGRVEAAVEASGSHVQITVRDTGQGIAPAFLPHVFDRFRQADASTARLH
                     GGLGLGLAIVRHLVELHGGAVGAESQGEGRGATFIVRLPVLGVFSPAVPAAVGTAREA
                     LLEPEASLPAPPRSSLLGLRVLVVDDHPDTLELVSTVLRGHGAEVAAASSTAEAKRVF
                     GDVRPHVLLSDIGMPEQDGYELIRYVRGLPEDRGGATPAAALTAYASPADRLKILDAG
                     FQAHIPKPVEPLDLVTLVVRLVGAR"
     gene            6866..8440
                     /locus_tag="VEL74_24940"
     CDS             6866..8440
                     /locus_tag="VEL74_24940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65851.1"
                     /translation="MPRFLILSILAALLALGFGGCSAREPGEGETTAEATPTPTPTPI
                     VPAPEYCAKAPHDQPCSFIPQDVTAKLNPGYDGLTENDQRPFDNFSWQMFLALNWPAD
                     ANGKPLPGSIGSNPTAPRVWMSYPKPVDVFHLDFPGGVDATPEVCQGVGDSPGLPVFQ
                     LMAKNDHGGVGGSILEATGQPLIDRNLNFTLFDVRLNDVEANYIRSNGLDTLGGQVQF
                     KQAGKTVSFPMGYYQDPNRRLGGSVGAIEIKSAWRIMDAAKGDRLDRFYTTPALVYVK
                     AENSETGKPFCFQATLGMVGFHAIQRTTGPGGQEQDWMWGTFEHVDNQPEALDAADPT
                     SLRPAPSNNCTAPPRAPNIYSYYQATCNGPGCTPNTAPQPEPNGNYLWATTPPYARRY
                     GNGDRKNFGTQVVRCWKIYPETADLNAAFAKKLAGTVWANYRLINTQWQAHKDSPEVV
                     GQVPAFLGNPVQETYIQSNATCLGCHGFATTAVGQDANFSFLLSIPRLFATEGQEGAA
                     GTAAPSPPAAGAGTGG"
     gene            8603..9073
                     /locus_tag="VEL74_24945"
     CDS             8603..9073
                     /locus_tag="VEL74_24945"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HYG65852.1"
                     /translation="MSVESNKAACLEFWRRAYQEGHPEFMYQALAGDAVDHELEPFRV
                     PYMSTAQLAVGFTRVYLKAFPDLQVDIEDMLGEEDRVMTRWRMRGTQTGPLMGIEASG
                     RPIDITGLRVDRFVNGKIAETWGNWDLLGLLEQIGALPPLRRGLFPALRSLAAA"
     gene            9161..10060
                     /locus_tag="VEL74_24950"
     CDS             9161..10060
                     /locus_tag="VEL74_24950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HYG65853.1"
                     /translation="MRIAVLGAGGVGGYFGGALARAGHPVALLARGAHLEALRQRGLE
                     VRTPEETYTVAVEAVDDPEGLGPAGLVLVAVKSYSLPEVAPAARLLAERGAVVVPLLN
                     GVEAADRLVESGVPAGQILGGLTEISAVRTGPGVVERRSPFQRVVVGERAGGASERAE
                     RIAAAFREAGAEGRVSTDITLDLWRKFAFISAMSAACGLARAPVGTIRSAPLGHLLIE
                     RALREVVAVGQARGVALTGEDEARILDFVDTMPGGLKPSFLLDLEAGGPTEIDDLSGA
                     VSRLGRLAGVETPLHDAATAALR"
     gene            complement(10522..10794)
                     /locus_tag="VEL74_24955"
     CDS             complement(10522..10794)
                     /locus_tag="VEL74_24955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65854.1"
                     /translation="MILLTKTEEILLVQFHEMKGERVGARPGDKVPASYFKKTEEDTK
                     GLEFEGAMDTLIAHNLVKKEGDDYVLTQEGYDYMYTREGGRRIDEG"
     gene            10954..11322
                     /locus_tag="VEL74_24960"
     CDS             10954..11322
                     /locus_tag="VEL74_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HYG65855.1"
                     /translation="MGDTTVTKIDSRHSPVGEMGQRYLASGVHVAMRLWEEEPGEGKP
                     ASRRDYETVGFVIDGRAELTLEGQTVTLEPGNSWVVPKGAEHRYRVLERFKAVEATSP
                     PAHAHGRDEEMGRGEISGTD"
     gene            complement(11346..>11702)
                     /locus_tag="VEL74_24965"
     CDS             complement(11346..>11702)
                     /locus_tag="VEL74_24965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HYG65856.1"
                     /translation="ELDYDKVLPLIAFDGHRIVADATLHYREGGPLRLVGRVKWLIEP
                     RYRGQGLGTILINNFISTARNNGLRHLTCMLISDLEADAVATLTNLGFQSYPVPGYGT
                     DPDGNQHDMVKMVLKL"
BASE COUNT         1720 a         4168 c         4111 g         1703 t
ORIGIN      
        1 cccaactcgg agacggagtt ctatttcaag gaggccgact cccggttcgt cttcgtccgc
       61 ggccccaacg gcaaggcggt gcgggtcatc ttctcccagg gaagcctgga gctgccggcg
      121 ccgcgggtgc agtagaagga ggggacccgc gccgccgggc gttccggccg gctcggccgt
      181 tgcatcccgg aattctacag gctcccggcg tcaggtgtcg gaaaagggta tactgccatt
      241 tctccatctg ttacgagccc accggagaat caatatgatt gaactagcga agagccgcct
      301 gccagagatc ctcggccggc acgaacgtga gattctggaa gagtggatgc agcaacagct
      361 tgctgctgct acgaaccgcc gggacctcat ccaggagcgt gagctggccg agcaatcccg
      421 cgacttcctg gctgctttcc gtagcgccct cacccaggac ggcgcgggcg acgtggacgg
      481 cccggcgtgg ggcggcgtgc gggaaatcct cgccgagctg tcgcgcagcc gggcccgcaa
      541 ggggttttcg tcctcggaga ccgcgacctt cgtgttctcc ttgaagcagc cgctgttcga
      601 gcgcctccgc cagcacctgg gacaggacgg cgcggccctc ggcgaggcca tctggagcct
      661 gacgaccctg ctcgacaacc tcggcctgtg gacgaccgag gtctaccaga aggggcgcga
      721 ggaggtgatc catcgccagc agcaggagct gctggagctc tccacgccgg tggtgcggtt
      781 gtggaccaac atcctggccc tccccctgat cggcaccctg gacagcgccc gcacccaggt
      841 ggtaatggag aacctgctgc aggagatcgt caacgccggc gccgccatcg ccatcatcga
      901 catcaccggc gtccccaccg tcgacaccct ggtggcacag cacctgctga agaccgtcgc
      961 cgccgcccgc ctgatggggg ccgactgcat catcagcggc atccgcccgc agatcgcgca
     1021 gaccatcgtg cacctggggg tcgagctcgg agacgtcacg accaaggcca gcctggccga
     1081 cgcctttgcc ctggccctcc gccggaccgg cctcaaggtg accaccgcgc agccgatgat
     1141 gcagccggcc gccggcgctt gacggaggag cttcgtggac aggatcccga ttctgaagat
     1201 gggcgagttc ctcctggtca ccatccaggt cgacatgcac gaccgcctgg tgatgacgct
     1261 ccaggaggac ctgatggagc agatccgccg gaccgaggcg cggggcgtgc tgatcgacat
     1321 ctcggcgctc gacgtcgtcg actccttcat cggccgcatg ctcggccaca tcgccgcgat
     1381 ggcccgggtg ctcgacgccc agacggtggt caccgggatg aggccggccg tggcgatcac
     1441 gctggtggag ctcggcctgt cgctcaaggg ggtgcgcacc gccctggacg tggagcgcgg
     1501 tatggatctg ctccggacgt ccgtcgagga gcaggagaac gtccgtggca gtctcgcgac
     1561 ctgaggagct gccgatccag cgcgaggagg acgtcgtgcg cgtgcggcag aaggtccggg
     1621 agtgggcggt cgacctgggc ttcggtctcg tcgaccagac caaagtcgtc accgcggcgt
     1681 ccgagctggc gcgcaatacc atgctgcatg gcggcggcgg ccgcgtcctc ctggaaatgg
     1741 tgacggaggg gacacgccgc ggtctgcgcc tcaccttcga ggaccaggga cccggcatcc
     1801 ccgacctcga cctcgcgctg aaagacggct tcaccaccgg cagcggcatg gggctcgggc
     1861 tgggaggctc caagcggctg atgaacgagt tctcggtctc gtcggtcgtc ggggcaggga
     1921 cgcgggtgac cgtggtgaag tggaagtgag cctcccccgg atgtgggacg gcgaggtcat
     1981 cgacttcgcc gtgccggtga ccgaggtcag ccaggtcgga gaggcgcgcc ggctgatttc
     2041 caccgtcacc cgccggctcc gcttcgacga gacggcggcg ggcaaggccg ctatcgtggc
     2101 gacggagatg gccggcaatc tggtcaagca cggcggcggc ggcgagatca tcgtccgccc
     2161 gctggcgcat ggggaacggc aggcgctgga gatcctggct ctcgaccgcg gccccgggat
     2221 cgccgatgtg gcgaagtccc tgcgcgacgg gtattccacg gcgggcaccc ctggcaccgg
     2281 cctgggcgcg gtggcgcggc tctccgacgg attcgacctc tactcggccc cgggccaggg
     2341 gacggcactg ctctcgcggt tgtggtccag ggccgccccc ccgggtggca cggacggctt
     2401 ccacgagccg gccgagcacg gggccgtctg cctgccggtc acgggcgagg aggcgccggg
     2461 ggacagctgg gagatccagc aacggccggg cggctggcgg ctcctcgtcg ccgacggcct
     2521 ggggcacggc cccttcgccc gcgaccccgc gcagcgagcc atcggcgcgt tccgggagac
     2581 tccggccggc tcgcccgccg aggtgctgga ggcctgtcac gcggcactgc acggcacgcg
     2641 cggcgcggcg gtggccgtgg ccgacgtgga cctcgaggcg gggaccgtcc gctttgccgg
     2701 cgtcggcaac gtggcggcca ccatcctggg ggaggggcct tccctctaca tggtgtcgct
     2761 caacggcatc gtcggccagg gcgcggcacg gttccgcgag ttcagctatc cttggtcccc
     2821 gggggctctg ctggtgatgg cttcggacgg cctgagcacg cgctggaagc tcgattccta
     2881 cccgggcctc gccgcccgcc atccgagcct gatcgcggga gtgctctatc gcgatcacgg
     2941 ccgccctcgc gacgacatca cggtcgtcgc cgtccgcgcc gcccagctcc ggcgcggcgc
     3001 tggaggaggg gcccggtgag gccggcgctg ctgaccgtcg acatccgcca ggaggccgac
     3061 gtggtggtgg cgcggcagcg gtcacgccag ctcgcgcgcc tgctcggctt cgacgcccag
     3121 gaccaggtgc gcatcgccac cgccgtctcc gagatcgcgc gcaacgtcta tcagtatgcc
     3181 gggcgggggc gcatcgagtt ccagctcgag gggcgcaccc ctccccaggt gttcgtcgcc
     3241 cgcttgaccg accaggggcc ggggatcgcg gacgtgccgc tcatcctctc cggccgctat
     3301 cgctcgcaga ccggcatggg gctgggcatc accggcgccc ggcgcctcat ggaccagctc
     3361 cagatcgagt cggctcccgg caagggcacc acggtctggc tgaagaagct cctccccgcg
     3421 ggcgccccct acctcgacgg cccgcggctg gcggcggtcg gcgacgccct ggcgcgggag
     3481 ggcttccaga accccctggc cgaggtccag aaccagaacc aggagctcct ccgcgccctc
     3541 ggcgagctgc gcaaccggca ggacgagctg ctgcgggtca accgcgagct cgaggacacc
     3601 aaccgcggcg tggtggcgct ctatgccgag ctcgacgaga aggccgagca cctgcggcgc
     3661 gccgacgaga tgaagtcgcg ctttctgtcg aacatgagcc acgagttcag gacccccctg
     3721 aactcgatcc tcgccctgtc gcgcctgctc gaggagcgca ccgacggcga gctgacgggc
     3781 gagcaggcga agcaggtggg cttcatcaag aaggcggccg gcgacctcac cgagctggtc
     3841 aacgacctcc tggacctggc gaaggtggag gcgggcaaga cggtcgtgcg gccgatcgag
     3901 ttcgaggccc ggaacctgtt cggcgcgctg cgcggcatgc tgcgcccgct cctcgtcgcc
     3961 gagagcgtga acctggtgtt cgaggaccag gcggacctgg cgcccctcta caccgacgag
     4021 gcgaaggtct cgcagatcct gcgcaacttc atctccaacg cgctcaagtt caccgagcgc
     4081 ggcgagatcc gcgtctcggc gaccctgagc cacgacctgg acggcggcgt cgagggggtc
     4141 tgcttcgcgg tcgccgacac cggcatcggc atcgcgcccg aggaccacga gcggatcttc
     4201 cgcgagttcg accaggtgga gggccggctg caggcgaagt tcaagggcac gggcctgggg
     4261 ctcgccctgt ccaagcggct cgccgagctg ctcggcgggc gcatcaccct ggagagcacc
     4321 ctcggcgccg gctcgacctt ctcgctcacc atcccgcgcg tctacgagtc ggacgagccg
     4381 gtcgaggagg agccgctggc ggaggagtgg catcccgacc cccgccgcct gccggtgctg
     4441 atcgtcgacg acagccccga gagcctgcac gtctacgagc ggctgctgcg cgacaccccg
     4501 ttccagcccg tctccacccg caccctgcgc gaggcggcgg cgcggttgcg caccgtgcac
     4561 ccggccgcca tcgtcctcga catccagctc gtgggcgagc agacctggag cttcctcgcc
     4621 gggctcaagg gggccgacga gacccgggac atcccggtgc tggtgatcac cagcgtcgac
     4681 gatccggcca aggccgccgc gctcgccgcc gacggctact gcgacaagcc ggtcgaccgc
     4741 gactggctgc tcgaaaggct gggcagcctg acgaggaagg ccggcaaggc gctgatcatc
     4801 gacgacgagg aggcctcccg ttatatcctg aaggggctgg tggtcaggct gccgctggag
     4861 ccggtcgaga gctcggacgg cagggagggg ctgcggcggg cccgcgagga gcggcccgac
     4921 gtgatcctcc tcgacttggt gatgcccggc ctcaccggct tcgatgtgct cgacgagctg
     4981 agagctgatc catccacccg cgaaatcccc gtggtcgtct ccacctccaa gatcctggcc
     5041 caggaagagc gccagcggct cgagcggtgg tcggtccccg tcctctccaa ggagacctgg
     5101 gcgcggccgg acgcggtgac ggagctccgg caggcgctgt cgcgggccgg ctggagctcg
     5161 ggctccgccg aggagcgggc gtgaaacccc agccggtcat cctccatgtc gaggacaacg
     5221 aggcggtgcg ctacggcatc ggccgggtgc tgctgcggga ggggttcgtt ctccaggagg
     5281 cgcccaccgg cgaggagggg ctgcggcgcg cccgtgagtc ccccgacctc atcctcctgg
     5341 acgtcaaact gcccgacatg agcggcttcg acgtctgcaa gctgctcaag tcagacccgg
     5401 agaccttctc cattccggtg ctgcacctca cctccacgta cggcagcagc gagcacctgg
     5461 ccgcagggct cgaaggcggc gccgacggct acctcaccca tccggtggag ccgatcgtcc
     5521 tggtggcgac gatccgcgcc ctgctccgtg cccgcgtcgc cgagcggcag gtgcaggagc
     5581 tgctgcggct cgaggcggcc gcgcgcgccg ccgcggagca ggcgaaccgg ctcaaggacg
     5641 agttcctggc caccctgtcc cacgagctgc gcacgccgtt gaacgccatc ctgggctggg
     5701 tgcagctcct gcgttccggc tccatggaca cggccggcgt cgcgcgggcg gtcgagacca
     5761 tcgagcgcaa cgccaaggcc caggcccagc tcatcgccga catcctcgag gtgtcgcgca
     5821 tcatcaccgg caagctgcgc ctcgacgtgc atccgctgga cctgcggtcg gtggtccgca
     5881 gcgccgccga gaccgtgcgg cccggcgccg aggcgaaggg ggtcgctctc gatctgtccc
     5941 tggaccggga cgtgccgccg gtctcggggg atcccgaccg cctgcagcag gtggtctgga
     6001 acctcctgtc caacgccatc aagttcaccc cccgcggcgg ccgggtggag gccgccgtgg
     6061 aagcgtcggg ctcccacgtc cagatcaccg tccgcgacac cggccagggg atcgcgcccg
     6121 ccttcctgcc gcacgtcttc gaccgcttcc ggcaggccga cgcctcgacc gcgcgcctcc
     6181 acggcgggct cggcctgggg ctggcgatcg tccgccatct cgtcgagctg cacggcgggg
     6241 cggtcggcgc cgagagccag ggagaggggc ggggagccac cttcatcgtg aggctgccgg
     6301 tcctcggcgt gttcagcccg gccgttccgg cggccgtcgg caccgcccgg gaggcgctgc
     6361 tggagccgga ggcctccctt ccggcgccgc cgaggtcctc cctcctgggc ctgcgggtgc
     6421 tcgtcgtcga cgaccacccg gacaccctgg agctcgtctc caccgtgctg cgggggcatg
     6481 gcgccgaggt ggccgccgcc tcttcgacgg ccgaggcgaa gagggtcttc ggcgacgtcc
     6541 gcccacacgt cctgctgagc gacatcggca tgcccgagca ggacggctac gagctgatcc
     6601 gctacgtccg cggcctgccg gaggaccgcg ggggcgccac ccccgcggcg gccctcaccg
     6661 cgtacgccag cccggccgac cgcctcaaga tcctcgacgc cggtttccag gcgcacatcc
     6721 cgaagccggt cgagccgctc gacctggtca ccctcgtcgt ccggctggtc ggcgcgcggt
     6781 agccggcttc ctgctagcat tccctccagt tcttttccac tcgccggcgc ctgccggctc
     6841 tgctgtcttc agaaaggaga tgaggatgcc gcgattcttg atcctctcca tcctggcggc
     6901 cctcctggcg ctcggattcg gaggctgctc cgcccgcgag ccgggcgagg gtgagacgac
     6961 cgcggaggcg acgcccaccc cgacccccac tccgatcgtc ccggcccccg agtactgcgc
     7021 caaggccccc cacgaccagc cctgctcgtt catcccccag gacgtcaccg ccaagctgaa
     7081 ccccggctac gacggtctga cggagaacga tcagcggccg ttcgacaact tctcctggca
     7141 gatgttcctg gcgctcaact ggccggcgga cgccaacggc aagccgctgc cgggctcgat
     7201 cggctccaac cccacggcgc cgcgggtctg gatgtcctac ccgaagccgg tggacgtctt
     7261 ccatctcgac ttcccgggcg gcgtcgacgc cactcccgag gtctgccagg gggtggggga
     7321 cagcccgggg ctgccggtct tccaactcat ggccaagaac gaccatggcg gcgtgggcgg
     7381 ctccatcctc gaggccaccg gccagccgct catcgaccgc aacctgaact tcaccctgtt
     7441 cgacgtgcgc ctcaacgacg tcgaggccaa ctacatccgc agcaacggcc tcgacacgct
     7501 gggcggccag gtccagttca agcaggccgg gaagaccgtg agcttcccga tgggctatta
     7561 ccaggacccc aaccggcggc tcggcggctc ggtgggggcg atcgagatca agagcgcctg
     7621 gcgcatcatg gacgccgcca agggggacag gctcgaccgc ttctacacca cgcccgcgct
     7681 ggtctacgtc aaggccgaga acagcgagac cggtaagccg ttctgtttcc aggccaccct
     7741 ggggatggtc gggttccatg ccatccagcg caccaccggc ccgggtggcc aggagcagga
     7801 ctggatgtgg ggcaccttcg agcacgtgga caaccagccg gaggcgctcg acgccgccga
     7861 tcccacgagc ctgcggccgg cgccgtcgaa caactgcacc gcgccgccgc gggcgccgaa
     7921 catctactct tattatcagg cgacgtgcaa cggccccggc tgcacgccca acacggcgcc
     7981 gcagccggag cccaacggca actatctctg ggccaccacg ccaccctacg cgcggcgcta
     8041 cggcaacggc gacagaaaga atttcggcac gcaggtggtg cgctgctgga agatctatcc
     8101 cgagacggcg gacctgaacg ccgccttcgc caagaagctc gccggcacgg tctgggccaa
     8161 ctaccggttg atcaacacgc agtggcaggc gcacaaggac tcgccggagg tggtcgggca
     8221 ggtgccggcg ttcctgggca acccggtcca ggagacctac atccagtcga acgccacctg
     8281 cctcggctgc cacggcttcg ccacgaccgc ggtgggccag gacgccaact tcagcttcct
     8341 gctgagcatc ccgcgcctct tcgccaccga gggtcaggag ggcgctgccg gtaccgcggc
     8401 gccgagcccg ccggcggccg gcgcggggac ggggggatag gcctcgccgg ggccccaccc
     8461 cttcgggtgg ggccctcccc atcctttcgg atttgctgga ctatccgatc ggcgatgtcc
     8521 cgcggcaccg cccgtcccta gaatcctctt catcacagtg accggcatcc tgccagcgac
     8581 tgattcatga aggggcctcc cgatgtccgt ggaaagcaac aaggccgcgt gtctcgaatt
     8641 ctggagaagg gcttaccagg aggggcatcc cgagttcatg tatcaggcgc tggccggcga
     8701 cgccgtcgac catgagctgg agcccttccg cgtgccgtac atgagcacgg cgcagctggc
     8761 ggtcggcttc accagggtct acctcaaggc cttcccggac ctgcaggtgg acatcgagga
     8821 catgctgggc gaggaggacc gggtgatgac gcgctggcgc atgcgcggga cccagacggg
     8881 ccccctgatg ggcatcgaag ccagcggacg gcccatcgac atcaccggcc tccgcgtcga
     8941 ccggttcgtg aacgggaaga tcgccgagac ctggggcaac tgggacctgc tcgggctgct
     9001 cgagcagatc ggcgccctgc cgccgctgcg ccgcggtctc ttcccggccc tgcggtccct
     9061 ggccgcggcg tgaccggacg gctcgaatcg gttttctccc tcgtgtagca tcgggggccg
     9121 ctcggacggt ccgatgcggc gagccgaggg aggtgaggtg atgaggatcg cggtcctggg
     9181 ggcgggtggc gtcggcgggt acttcggcgg cgcgctcgct cgcgccggcc acccggtggc
     9241 tctgctggcc cggggtgccc acctggaagc cctgcggcag cgcgggctgg aggtccgcac
     9301 gccggaggag acgtacaccg tggccgtcga ggcggtggac gacccggagg ggctcggccc
     9361 ggccggcctc gtcctcgtgg cggtcaagag ctactcgctg ccggaggtcg cgccggccgc
     9421 ccggctcctc gcggagcgcg gcgcggtggt cgtgccgctg ctcaacgggg tggaggcggc
     9481 ggaccggctc gtcgagagcg gcgtcccggc cggccagatc ctcggcggcc tcacggagat
     9541 cagcgccgtc cgcaccggtc cgggcgtcgt cgagcgccgc agccccttcc agcgggtggt
     9601 ggtgggggag agggcgggcg gagcctccga gcgtgccgag cggatcgccg ccgcgttccg
     9661 ggaggcgggc gcggaggggc gcgtttccac ggacatcacc ctcgacctct ggcgcaaatt
     9721 tgccttcatc agcgccatgt ccgccgcctg cggcctggcc cgtgcgccgg tcggtaccat
     9781 ccggagcgcg ccgctgggtc acctcctcat cgagcgggcg ctgcgcgagg tggtcgcggt
     9841 gggccaggcc cgcggcgtcg ccctcaccgg cgaggacgaa gcgaggatcc tcgacttcgt
     9901 cgacaccatg cccggcggcc tgaagccgag cttcctgctc gacctcgaag ccggcggccc
     9961 caccgagatc gacgacctca gcggcgccgt ctcccggctc ggccggctcg ccggggtcga
    10021 gacccccctg cacgacgcgg cgacggcggc gctgcgctga ccgcgcgttg cgggccgttc
    10081 caacggctcg ccgaagcctc cggcgagccg tcgacggctt cgccggcgcg ttgtaagcat
    10141 ccgaccggcc gccgaagctt ccagcgagct gccgaagctt ccggcggcct gccggagagc
    10201 acgacagccc gtcgtagtct tcggcggcgc gtcaaaggtt cccacggcgc gccggacggt
    10261 ccggcgggcc gtcgaagctt gcggcgcgct gccggagatt ccgcgggccc gccgaagctt
    10321 gcgacggccc gccggagagt acggcgccgc gccggagctt ccggcgagcc gccggactct
    10381 tcgccagggc gacggatcgc tccaacggcg cgccggaggc tccagcagcg cgccggagaa
    10441 gccgtccctt ccttatatag atggagaacg ccaccggcgg ggccgccggt ggcgcggggt
    10501 ggtcagggga gtgggggggc gctagccctc gtcgatccgc cggccgcctt cgcgggtgta
    10561 catgtagtcg tagccctcct gggtcaggac gtagtcgtcc ccttccttct tcaccaggtt
    10621 gtgggcgatc agggtgtcca tggcgccttc gaactccagg cccttggtgt cctcctcggt
    10681 cttcttgaag tacgaagccg gcaccttgtc gcccggacgg gcccccaccc gctccccctt
    10741 catctcgtgg aactgcacca gcaggatctc ttcggtcttg gtcagcaaga tcatgggctg
    10801 tattcctccg tttcggtgcc gtgggtcccc ccccggctca tcttcccgga attgcatgga
    10861 gcgtgccgcc gtctggcaac ggtcgtgcag aagagcgagc tgacaccgcc tcctgccgcc
    10921 gggcggcggc cgcaacttcg gaaggagaag agaatgggcg ataccacagt caccaagatc
    10981 gattcccggc actccccggt cggcgagatg ggccagaggt atctcgcctc cggcgtccac
    11041 gtcgccatgc gcctatggga ggaggagccg ggcgagggca agccggcgag ccgccgcgac
    11101 tacgagaccg tcggcttcgt catcgacggg cgcgccgagc tcaccctgga ggggcagacg
    11161 gtgacgctcg agcccggcaa ctcctgggtc gtccccaagg gggccgagca ccggtaccgg
    11221 gtcctggagc ggttcaaggc cgtggaggcg acgtcgccgc cggcccatgc ccacgggcgc
    11281 gacgaggaga tgggccgggg agagatcagc ggcaccgatt agccggcgcc agccggcgcc
    11341 aggcctcaga gcttcagcac catcttcacc atgtcgtgct ggttgccgtc ggggtcggtg
    11401 ccgtagccgg ggaccgggta gctctggaag cccaggttgg tcagcgtggc gacggcgtcg
    11461 gcctcgaggt ccgagatcag catgcaggtg aggtggcgca ggccgttgtt ccgcgccgtg
    11521 ctgatgaagt tgttgatcag gatcgtcccc aggccctggc cccggtaccg gggctcgatg
    11581 agccatttca cccggccgac caggcgcagc ggccccccct cgcggtagtg cagggtggcg
    11641 tcggccacga tccggtggcc gtcgaaggcg atcagcggca gcaccttgtc gtagtccagc
    11701 tc
//
LOCUS       DASPJI010000885         8334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_571230, whole genome shotgun sequence.
ACCESSION   DASPJI010000885 DASPJI010000000
VERSION     DASPJI010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571230"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1799
                     /locus_tag="VEL74_24970"
     CDS             <1..1799
                     /locus_tag="VEL74_24970"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HYG65857.1"
                     /translation="AADLYRSLWTFFPDELDHGLNLAETLSTAGRNAEARAVIDELRR
                     LPEPEGQDPRIDLKEAEIAMRMADYAAQLKAAERAIEKGEELAEIQVMAEGLTYKGDA
                     LLLSGNPRESIVHYQRARTMFGQAGNKPAEAKLLLSLGVSLQQMSNLAEARDMIESAL
                     DAAEKQGSSRSISLLQANLAVLHMDLGDVRGARELIEPAYAAFKAEGDRVREEKALYV
                     LSTALLAQGDVAGSRQRLQMVRNLAQQTGNRTDLVVTLDDLGVLLARQNSPAQAMRLH
                     NQALAISRSLGDQGRIARVLISMGDTLTQMGALTRARQCYAEAFEHARAMGHRLLVAR
                     ASGGLSRLSYVQGDLATARRHAESELRIALDAGAQPIAIRARADLAQLYLAGGELDTA
                     RRYLDEALDTANSLGLELEKMTLHLRLARVALAEGDTQAAAALARETADWFGKRQLVA
                     DQARSLAVLAEALLTSKRLAEAQEVASQLRALAGNSDDLELRITAAVQAARIGSTAGE
                     VGESFMKMRQAEEMARNTGLVAAGLEARLVLGQFQLERLDRVAGREALEGLRREAEAK
                     GFWRVAGHAAEALQVNRSPRSPAPPGAPVAGR"
     gene            2006..2533
                     /locus_tag="VEL74_24975"
     CDS             2006..2533
                     /locus_tag="VEL74_24975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65858.1"
                     /translation="MKKALVLALAMVALGAISASATTVLYVPMNKSVDLSEVILLGHV
                     LRMEPAYNAEGEIVTRIDLLVEEGLKGGVERSAIFSFFARGGSLDGVHVETVGEAKYR
                     LGERVLVQLESIDGEYYTLGLAFGKWNVAQERDGRPVMIRNLSDLHMVGAEETPVDKF
                     PLQRMRDIAALRQAF"
     gene            2597..3196
                     /locus_tag="VEL74_24980"
     CDS             2597..3196
                     /locus_tag="VEL74_24980"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HYG65859.1"
                     /translation="MKKIVLMAAVLALSASLASAYSLNGRKWFSSSDATFRFNNSNGA
                     TCCLSASAQSSAIQSGLNAWAILSNGGSTNLSGAKRDGVNVVSWGKLGGTTLGVTNYI
                     ATDSSQSQVCGNNLIYRFQEVDVRFNNAFNWQTSSTCTNGFDLSGVSVHEFGHAVGLG
                     HSSVPGATMYPSVAACDFSVSSLANDDKAGYSAIYSGCR"
     gene            3325..5040
                     /locus_tag="VEL74_24985"
     CDS             3325..5040
                     /locus_tag="VEL74_24985"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HYG65860.1"
                     /translation="MAERIGGPAHVRSALFVDFDNIYLGLQREDPSAAEQFATNPAHW
                     LAWLQESLSPTDGSELGDAGAGGRRTRPNRKILFRRCYLNPSQFGRFRPYFTRSAFEV
                     VDCPPLTSGGKTSADIHMVMDILDTLGHATCFDEFIILSGDADFTPVLLRLSKHDRRS
                     AVLAIGPASVAYKAACDLLIDQDTFLEDAIGVAPSPAGRAGEPPRSSAMEDLLRRIAD
                     KVQERALTVGELVATDLPGIFREFPEFTASTNWLGFNSLRGMATAVTQARPDLIMVEG
                     DPWKVRTGDPSEKEAVVLSGERTPRLRPAVDYGRLTEEILEYVRSVVETSENAVPMAR
                     AAQMVISRFGEEVLDSRWAGFGTFRDLLEGRAEPGFSISSLKPGYIYDPERHQLPSDG
                     HPEELALGDPHLNSLAFRIHQLTDTPYLRGDEYARVFRLIAREVNEHGYFLTRTSKAV
                     RDRCLDEDSPIARASVNFILRGITFSGHRFGQNGPEDPARLGDCFFRNVLSLCESAGL
                     SLDSGERDVLRSWILGQLDEGETPAADASGPEAADEEDDDTGEPLPVEVKAEAAATLL
                     EADEA"
     gene            5037..5459
                     /locus_tag="VEL74_24990"
     CDS             5037..5459
                     /locus_tag="VEL74_24990"
                     /inference="COORDINATES: protein motif:HMM:NF022495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HYG65861.1"
                     /translation="MSGGAGWELGEDEARDALALPAQGRYTLFLQLACDWEEAWGLRN
                     PDGWVVASGPEGDSFPLWPHPHLAQACARGPWEDAAPAAIPLDELLDDLLPLLAEDHV
                     KVAAFPTPDGESWVVAPDELRRGLEAELALGQSGEESP"
     gene            5503..5871
                     /locus_tag="VEL74_24995"
     CDS             5503..5871
                     /locus_tag="VEL74_24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HYG65862.1"
                     /translation="MADPLTAAAPISQEERTWALAAHLSALVGSVIPLGNIVAPLVIW
                     LVQKDKMPFVDDQGKEALNFQISVTIYLLACLPFWLILIGIPMMIAVGLGAVVLMIVA
                     AVKSNEGIRFRYPLTLRFIR"
     gene            5890..6279
                     /locus_tag="VEL74_25000"
     CDS             5890..6279
                     /locus_tag="VEL74_25000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HYG65863.1"
                     /translation="MTDTVSTDDKSWATLCHISSLAGWAVPFGHILAPLVLWLIKRDG
                     RPLVDDQGKEAVNFQISFTLYMALVLAPAALLVFLQGPPRHFSFMPFAILAAVFLFAG
                     LALVVIGAVRAYHGEPYRYPFTLRLIR"
     gene            6295..6714
                     /locus_tag="VEL74_25005"
     CDS             6295..6714
                     /locus_tag="VEL74_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HYG65864.1"
                     /translation="MVKRLSVRSPGQGLHEITREVAAAVAEAVRASGVREGLCTVFLQ
                     HTSASLTIQENADPSARRDLESWLNRLVPERDPLYTHTAEGPDDMPSHIKAALTAASL
                     SIPILDGELGLGTWQGIYLWEHRRHPGARHVLVHVGE"
     gene            complement(7749..>8334)
                     /locus_tag="VEL74_25010"
     CDS             complement(7749..>8334)
                     /locus_tag="VEL74_25010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HYG65865.1"
                     /translation="KAALGFLRRTLRPIDQAAVITFNEAPQVAVGFTNDMAALEEGFT
                     GLVAEDYTSLYDSIAFALHYLTGVKGQRAILLLSDGEDRSSRFGYEQILEYARRSGIT
                     LYAIGLDLPEGKRGEHARKLERLASETGGRSFFLTGTGELDAVYQEIERDLRAQYRIT
                     YQSSNTSTEDTFRAVDVQVAKSGLDARTISGYYP"
BASE COUNT         1252 a         3033 c         2813 g         1236 t
ORIGIN      
        1 aggccgccga cctctatcgc tccctgtgga ccttcttccc cgacgagctg gaccatgggc
       61 tcaacctcgc cgaaaccctg tccacggcgg gccggaacgc cgaagccagg gccgtgatcg
      121 acgagctgcg ccggctgccg gagccggaag gccaggaccc gcgcatcgac ctgaaggagg
      181 cggagatcgc catgcgcatg gccgactacg cggcgcagct caaggcggcc gaacgggcga
      241 tcgagaaggg ggaggagctg gccgagatcc aggtgatggc ggagggcctc acctacaagg
      301 gggacgcgct gctcctcagc ggcaaccccc gggagtcgat cgtccactac cagcgggcgc
      361 ggacgatgtt cggccaggcc ggcaacaagc cggccgaggc gaagctcctc ctcagcctcg
      421 gggtgtccct gcagcagatg agcaatctgg ccgaagcccg ggacatgatc gagtcggcgc
      481 tcgacgccgc cgagaagcag ggcagctccc ggagcatctc cctgctgcag gcgaacctgg
      541 cggtgctcca catggacctc ggcgacgtgc ggggcgcgcg ggagctgatc gagccggcct
      601 acgccgcgtt caaggcggag ggggaccggg tgcgggagga gaaggcgctc tacgtcctct
      661 ccacggctct cctggcccag ggggatgtcg ccggctcgcg gcaacggctg cagatggtgc
      721 ggaacctggc ccagcagacg ggcaaccgta ccgacctggt ggtgaccctc gacgacctcg
      781 gcgtcctgct cgcgcgccag aactcccccg cccaggccat gcgcctccac aaccaggccc
      841 tggccatctc ccgctccctc ggggaccagg gccgcatcgc ccgggtgctg atctccatgg
      901 gggacacgct gacccagatg ggcgcgctga ctcgcgcccg gcaatgctac gccgaggcgt
      961 tcgagcacgc ccgggccatg ggccaccgcc tgctggtcgc ccgggcctcc ggcgggctct
     1021 cccgcctctc ctatgtccag ggcgatctgg ccacggcgcg ccgccacgcg gagagcgagc
     1081 tgcggatcgc gctggacgcc ggagcccagc ccatcgccat ccgcgcccgg gccgacctgg
     1141 cgcagctcta tctggccggc ggggagctcg ataccgcccg gcggtacctg gacgaggcgc
     1201 tcgataccgc gaactctctc ggcctggagc tggaaaagat gacgctccac ctgcgcctgg
     1261 cccgcgtcgc cctggcggag ggcgacaccc aggccgcggc cgctctcgcc cgggagaccg
     1321 cggactggtt cggcaagcgc cagctcgtcg ccgatcaggc ccgctccctc gccgtgctgg
     1381 ccgaagcgct gttgaccagc aagcggctgg ccgaggcgca ggaggtcgcc tcccagctcc
     1441 gcgccctcgc cggcaacagc gatgacctcg agctgcggat caccgcggcc gttcaggcgg
     1501 cccgcatcgg ctccacggcg ggcgaggtgg gagagtcctt catgaagatg cgccaggccg
     1561 aggagatggc gaggaacacc ggcctggtcg ccgccggtct ggaggcccgc ctggtcctcg
     1621 gccagttcca gctcgagcgc ctggaccgcg tcgccggccg ggaggccctc gagggcctgc
     1681 ggcgtgaggc cgaggccaaa ggcttctggc gggtcgccgg acacgccgcg gaggccctgc
     1741 aggtgaaccg ctccccccgt tccccggccc cccccggtgc gccggtcgcc ggtcgttgag
     1801 agctggctca aattttgatc ccccctgtca gagaatgacc cgcccgcggc tcacttcctg
     1861 acccagtcgc cgctcccggc ggcggtaaat cccgtggaat cagtcctctc cggagtggca
     1921 cgcttcttgg gtaagaacgg cccgatacct tttcagccga gcgccgacaa ccggccccgg
     1981 cctctttgag gagagggaga cttccatgaa gaaagccctg gttctcgcct tggcgatggt
     2041 ggcgctcggc gccatctccg cctccgccac gacagtcctg tacgtcccga tgaacaagtc
     2101 cgtcgacctg agcgaggtca tcctcctcgg ccacgtcctc cgcatggagc cggcctacaa
     2161 cgcggagggg gagatcgtca cccgcatcga cctgctggtc gaggagggcc tcaagggcgg
     2221 cgtcgagcgc agcgcgatct tcagcttctt cgcccggggc ggcagcctcg acggcgtcca
     2281 tgtggagacc gtgggcgagg cgaagtaccg gctgggcgag cgggtcctgg tgcagctcga
     2341 gtcgatcgac ggcgagtact acaccctcgg cctcgccttc ggcaagtgga acgtcgctca
     2401 ggagcgggac ggccgcccgg tgatgatccg caacctgtcg gacctgcaca tggtcggcgc
     2461 cgaggagacg ccggtcgaca agttcccgct gcagcggatg cgcgacatcg ccgccctgcg
     2521 tcaggcgttc tgatccgccg ccattagaga cgagaaaccg actttctaga cctgcgccga
     2581 gcgcacgagg aaatccatga agaagatcgt tctgatggcc gccgtcctgg ctctctccgc
     2641 ctccctcgcc tccgcctaca gcctcaacgg ccggaagtgg ttctccagca gcgacgccac
     2701 gttccgcttc aacaacagca acggcgcgac ctgctgcctg agcgccagcg cccagagcag
     2761 cgccatccag tccggcctca acgcctgggc catcctgagc aacggcggct cgaccaacct
     2821 ctccggcgcc aagcgggacg gcgtcaacgt cgtctcctgg ggcaagctgg gcggcaccac
     2881 cctgggcgtg accaactaca tcgcgaccga cagcagccag tcccaggtgt gcggcaacaa
     2941 cctcatctat cgcttccagg aagtggacgt gcgcttcaac aacgcgttca actggcagac
     3001 gtcgtcgacc tgcaccaacg gcttcgacct gtcgggcgtg tcggtgcacg agttcggcca
     3061 cgccgtcggg ctcggccact cgagcgtccc gggggccacg atgtacccct cggtcgccgc
     3121 gtgcgacttc tccgtttcgt cgctcgccaa cgacgacaag gccggctaca gcgccatcta
     3181 cagcggctgc cgctagacgg cggccggttc ggcaaaggcg cgccggagcc ttccggcgcg
     3241 ccgcacctcc ccctgcctgg cccgcctcca atccagtaga cttgtcgcag ccgtgcggct
     3301 ggcgttgctg acttggagga tttgatggcg gaacgaattg ggggcccggc ccacgtgcgc
     3361 tcagcgctct tcgtggactt cgacaacatc tacctcgggc tccagcgcga agatcccagc
     3421 gcggccgagc agttcgcgac caacccggcc cactggctag cctggctgca ggagagcctc
     3481 tcccccaccg acggcagcga actcggagac gccggcgccg gcggccggcg gacacgcccg
     3541 aaccgcaaga tcctcttccg ccgctgctat ctgaacccgt cgcagttcgg ccgcttccgc
     3601 ccctacttca cccgctcggc cttcgaggtg gtggactgcc cgccgctgac cagcggcggc
     3661 aagaccagcg ccgacatcca catggtcatg gacatcctcg acaccctggg ccatgccacc
     3721 tgcttcgacg agttcatcat cctgtccggc gacgccgact tcaccccggt gctgctgcgc
     3781 ctctccaagc acgaccgccg cagcgcggtg ctggcgatcg gccccgcctc ggtggcctac
     3841 aaggccgcct gcgacctgct gatcgaccag gacacgttcc tggaagacgc catcggcgtc
     3901 gcgccgagcc cggcggggcg ggcgggggag ccgccccgga gctcggccat ggaggacctg
     3961 ctccggcgca tcgccgacaa ggtccaggag cgggccctga cggtgggcga gctggtggcc
     4021 accgacctgc cgggcatctt ccgcgagttc ccggagttca ccgccagcac caactggctg
     4081 ggcttcaaca gcctgcgcgg catggccacg gccgtgaccc aggcgcggcc cgacctgatc
     4141 atggtcgagg gggacccctg gaaggtgcgg accggcgatc cgtccgagaa ggaggccgtc
     4201 gtcctgtccg gcgagcgcac gccgcgcctg cgcccggcgg tggactacgg ccggctcacc
     4261 gaggagatcc tggagtacgt ccgctcggtg gtggagacct cggagaacgc ggtgccgatg
     4321 gcgcgcgccg cccagatggt catcagccgc ttcggcgagg aggtcctcga ttcgcgctgg
     4381 gccggcttcg gcaccttccg cgacctgctc gaggggcggg ccgagccggg cttctccatc
     4441 tcctccctga agccgggcta catctacgac cccgagcgcc accagctccc ctccgacggc
     4501 catcccgaag agctggcgct gggcgatccg cacctcaact ccctggcctt ccggatccac
     4561 cagctcaccg acacccccta cctgcgcggc gacgagtacg cccgggtctt ccgtctgatc
     4621 gcccgcgagg tcaacgagca cggctacttc ctcacccgca cctcgaaggc ggtgcgcgac
     4681 cgctgcctgg acgaggactc ccccatcgcc cgggcgagcg tcaacttcat cctccgcggc
     4741 atcaccttca gcggtcaccg cttcggccag aacggccccg aggatcccgc gcgcctcggc
     4801 gactgcttct tccgcaacgt gctcagcctc tgcgagagcg ccggcctgtc cctcgacagc
     4861 ggcgagcgcg acgtgctccg cagctggatc ctcggccagc tcgacgaagg ggagacgccc
     4921 gccgcggacg cctccgggcc ggaggccgct gatgaagagg acgacgacac cggcgagccg
     4981 ctgccggtag aggtaaaagc ggaggccgcc gccaccctcc tggaggcgga cgaggcgtga
     5041 gcggcggggc gggctgggag ctgggcgagg acgaggcgcg ggacgccctc gcgctgccgg
     5101 cccagggccg ctacaccctc ttcctgcagc tcgcctgcga ctgggaggag gcatgggggt
     5161 tgcgcaaccc ggacggctgg gtggtggcca gcggtcccga gggggactcg ttccccctct
     5221 ggccccaccc ccacctcgcc caggcctgcg cccgcggccc ctgggaggac gcggcgcccg
     5281 ccgccatccc cctggacgag ctgctcgacg acctcctgcc gctgctcgcg gaggaccacg
     5341 tgaaggtcgc cgccttcccc acgcccgacg gggagagctg ggtggtcgcg ccggacgagc
     5401 tccgccgcgg cctggaggcg gagctggcgc tcgggcagtc gggggaagag tccccttgaa
     5461 acgaatcggt ggtggatgac atagagagat aggaggaaga cgatggccga cccgctcacc
     5521 gccgccgccc cgatcagcca ggaagagagg acatgggccc tggccgctca cctgagcgcc
     5581 ctcgtgggct ccgtcatccc gctcggcaac atcgtcgcgc cgctggtgat ctggctggtc
     5641 cagaaggaca agatgccgtt cgtggacgac cagggcaagg aggcgctcaa cttccagatc
     5701 agcgtgacga tctacctcct ggcgtgcctg cccttctggt tgatcctcat cggcatcccg
     5761 atgatgatcg ccgtcggcct cggggccgtc gtcctgatga tcgtcgcggc ggtcaagtcc
     5821 aacgagggca tccgcttccg ttacccgttg accctccgtt tcatccgctg aaccggaaag
     5881 gagccggcca tgaccgacac cgtttcgacc gacgacaaga gctgggccac gctctgtcac
     5941 atctcctctc tcgcgggctg ggccgtgccc ttcggccaca tcctcgcccc tctcgtgctc
     6001 tggctgatca agcgcgacgg ccggccgctg gtggacgatc agggcaagga ggccgtgaac
     6061 ttccagatca gcttcacgct ctacatggcg ctggtcctgg cgccggccgc cctcctcgtc
     6121 ttcctccagg ggccgccgag gcacttcagt ttcatgccct tcgcgatcct cgcggccgtg
     6181 ttcctgttcg ccggcctggc cctggtggtg atcggcgcgg tgcgcgccta ccacggcgag
     6241 ccgtaccggt atccgttcac gctgcggctg atccgctgac gctgtccgct ggcgatggtg
     6301 aagaggctct ccgtccgttc cccggggcag gggctgcacg agatcacccg cgaggtggcc
     6361 gcggcggtgg ccgaggcggt ccgggcgagc ggcgtgcgcg agggcctctg caccgtcttc
     6421 ctccagcaca cctcggccag cctcaccatc caggagaacg ccgatccctc ggcccggcgc
     6481 gacctggaga gctggctgaa ccgcctagtg ccggagcgcg acccgctcta cacccacacc
     6541 gccgaggggc cggacgacat gccctcgcac atcaaggcgg cgctcaccgc cgcctccctc
     6601 tccatcccca tcctggacgg cgagctcggc ctcggaacct ggcagggcat ctacctctgg
     6661 gagcaccgcc gccaccccgg cgcccgccac gtgctggtgc acgtcgggga gtaggcccgg
     6721 ggctcgcggc ctcgtccgga tcgcgcgggc tccatccttt ttgaatcggg cgcgatccga
     6781 aggcttcggg aagcttccgc ggcgtccgga atccagccga ttgaattggg ctccctccaa
     6841 gggatctgga tgcgatccga ggcgctcggg aagattccgc ggcgtccggg ctccatcctt
     6901 ttgaagcgga cacgatccat ggcgcccggg aagcatccgg agcgtccggg ctccatccac
     6961 gccgttggga tgagatccac ggcgtgcgga tggcatccgg aggccccggc ccgcctccgc
     7021 ggcgacgggc tcccgagaag gatgcctgcg ccgcttcgcg gctgatggcg gcgcgtcgtt
     7081 gcaagcctcc aacgcgctgt cggagtttcc ggcgatccat tggaagcttc cgacgcgctg
     7141 tcggagtctc cggcgggccg tcgaagctgc cggcgggctg ccggaagctt cggagctctg
     7201 ccggagcttc cggcaggacg tcgcaaactc tgacgggcca atggaagctt ccagcgagct
     7261 gtcggagttt ccggcgggtt gtcgagctct ccggcacgct gtcgaagcct ccggcgggct
     7321 gtcgaagctt ccggcgcgcc gttggaagct tcggcggcgt gccggagcct tcggcgtgcc
     7381 gttggaagct tgcgactcgc tgccggagct tccggccggc cgccggaagc tccggccaga
     7441 agaaagcgcc ttccccgcga cggcggagaa ggcgctgttt cctgcgagcg atcggcgggc
     7501 cgcgcgaggc ggcggccgtc gtggcggcgg cgtcagacct cgagaaaggt gacgcccgtc
     7561 tcgctcgacg ggtccgcgtc ctcgacgacc cgcaccaccg accagcgacg ccgggggcgg
     7621 cgaaaaaccc ccctccttga cctcccccgc tccctcccgc ccaccctccc agggggggag
     7681 gcaccaccgc ccgggagggg gcaagccctg ggccagcccc tcccctacat ctatatagat
     7741 aggaggggct acgggtagta gccgctgatc gtccgggcgt cgaggccgga ctttgccacc
     7801 tggacgtcga cggcgcggaa ggtgtcctcg gtgctggtgt tcgacgactg gtaggtgatg
     7861 cggtactggg cgcgcaggtc gcgctcgatc tcctggtaga cggcgtcgag ctcgccggtg
     7921 ccggtgagga agaagctccg cccgccggtc tcggaagcca ggcgctcgag cttccgcgcg
     7981 tgctcgccgc gcttgccctc cggcaggtcg aggccgatgg cgtagagggt gatgccggag
     8041 cggcgggcgt actccaggat ctgctcgtag ccgaagcggc tggagcggtc ctcgccgtcc
     8101 gacagcagca ggatcgcccg ctgccccttg acgccggtca ggtagtggag ggcgaaggcg
     8161 atgctgtcgt agagcgacgt gtagtcctcg gccaccaggc cggtgaaccc ctcctccagc
     8221 gccgccatgt cgttggtgaa gcccaccgcc acctgcggcg cctcgttgaa ggtgatcacc
     8281 gcggcctggt cgatgggccg cagcgtgcgc cgcaggaacc cgagggccgc cttg
//
LOCUS       DASPJI010000886         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_169751, whole genome shotgun sequence.
ACCESSION   DASPJI010000886 DASPJI010000000
VERSION     DASPJI010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169751"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VEL74_25015"
     CDS             <1..752
                     /locus_tag="VEL74_25015"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HYG65866.1"
                     /translation="PAGLLAHSAVQRFGGQARFVVENYGDSELAKRFGVTRYPAIFVD
                     DILVATPKDFGFYGKGEGAGDGRYTPFGNPASHERFQGDLARMIELVLAGRKEDARAE
                     AAPRSASAGEIAALPAVSFTTLDGKALTRDDLSGKVVLVELWATWCPPCRGTLGWLGE
                     LKKRHGDRLTVVAMAVESDEADVRKLAGELNLPLVWTMGTPEVVRAFGDVSAVPTLLV
                     FDRDGRTAATFYGAPPSLHSEAEAKLASLLE"
     gene            794..1903
                     /locus_tag="VEL74_25020"
     CDS             794..1903
                     /locus_tag="VEL74_25020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65867.1"
                     /translation="MSPEPPIDPGEDSGNEPEASGPEEAAPEETASPPRKKRRRPRRG
                     QELPVRYLLDSRLFRTLCLPGREGALEGFRASLQRHGLAPECGLPPLELTPMAFLEVI
                     DIELPELDPFPLPASVLKSGESVMATSVIVRLAMDRFRESPGISVERLTQRVEELRPA
                     AAPAAHDLFDLCLAGFVSREEFQEDVYRHMAFDYLYRFQFPEVLREEVFEFLCASLFA
                     AGMNVAGLSKMRIIKQIWDRAYERLLKGNVGARGEIQALDREMKLRTRKDYLEWEAVH
                     YAVLGHPDDDDWGHPVTAFTPDPEERCRTRAIAYKTALRAFLDQIERSDLANIRPHLD
                     AWRPGVLVPCREDGTFETPIATGELPLYLAARKGG"
     gene            complement(1900..>2797)
                     /locus_tag="VEL74_25025"
     CDS             complement(1900..>2797)
                     /locus_tag="VEL74_25025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HYG65868.1"
                     /translation="RCLAALARLDYAPFEVLVVDNAPRGGTASAAKGIVAASGRRYVL
                     EERPGLDRARNRGAAEARHDIVAYTDDDCEPDPGWLAGLAAAFADPRVAGVTGRVLPA
                     AVETPAQRLFESYGGGMDKGATARELRWDRLGAFQRIAVQSVGVGANMAFRRAALAVV
                     SGFDPALDLGTPAHGAGDLDIFHRLLRAGCVLRYEPAALVRHHHRREMPQLRRQLRDN
                     GRSFGVYLMKRWREGDARTRGAVAAYALLRWAPWLASRVARGALGLHELPLPLLWEEL
                     AGALSAPRAWAEVRRIPSSSTG"
BASE COUNT          384 a         1029 c         1024 g          360 t
ORIGIN      
        1 atccagcagg tctgctggca cacagtgcgg ttcagaggtt cggcggccaa gcccggttcg
       61 tggtggagaa ctatggcgac tcggagctgg cgaagcgctt cggcgtgacc cgctaccctg
      121 ccatcttcgt ggatgacatc ctggtggcga cgcccaagga cttcggtttc tatggcaagg
      181 gggagggcgc gggcgacggc cgctacaccc cgttcggcaa cccggcgagc cacgagcgct
      241 tccagggcga cctggcgcgg atgatcgagc tggtcctcgc cggccgcaag gaggacgcgc
      301 gcgccgaggc ggcgccgcgg agcgcgtccg ccggcgagat cgccgcgctc cccgcggtca
      361 gcttcaccac cctcgacggc aaggcgctga cccgggacga cctatccggc aaggtcgtgc
      421 tggtggagct ctgggccacc tggtgcccgc cctgccgcgg cacgctgggc tggctcggcg
      481 agctcaagaa gcgccacggg gaccgcctga cggtcgtcgc catggccgtg gagtccgacg
      541 aggccgacgt ccgcaagctg gccggggagc tgaacctgcc cctggtctgg accatgggga
      601 cgccggaggt ggtgcgggcg ttcggcgacg tcagcgcggt gccgacgctg ctcgtcttcg
      661 accgcgacgg gcggaccgcc gccaccttct atggcgcgcc tcccagcctg cactccgagg
      721 ccgaggcgaa gctcgcctcg ctcctcgagt aggctcctgg agtaggatcg ggggcgcgcc
      781 ggcgcccccg atcatgagcc cagagccccc catcgaccca ggcgaggatt ccggcaacga
      841 gccggaagcc tccggtccgg aggaagccgc cccggaggag accgcctcgc ctccccgcaa
      901 gaaacggcgg cggccgcggc gcggccagga gctgccggtc cgctacctgc tcgacagccg
      961 gctgttccgg accctctgcc tccccggtag ggaaggggcg ctggagggat tccgcgcctc
     1021 gctccagcgt cacggcctgg cgccggagtg cggcctgccg cccctggagc tcacgcccat
     1081 ggcgttcctg gaggtgatcg acatcgagct ccccgagctc gatccattcc ccctgcccgc
     1141 gtcggtgctc aagagcggcg agtcggtgat ggcgaccagc gtcatcgtgc gcctcgccat
     1201 ggaccgcttc cgcgagtccc ccgggatctc ggtggagcgg ctcacccagc gggtggagga
     1261 gctgcggccg gcggcggcgc ccgcggccca cgacctgttc gacctctgcc tggccggctt
     1321 cgtgtcgcgc gaggagttcc aggaggacgt ctatcgccac atggccttcg actacctgta
     1381 ccgcttccag ttcccggagg tgctgcgcga ggaggtcttc gagttcctct gcgccagcct
     1441 gttcgccgcc gggatgaacg tggcgggcct gtcgaagatg cggatcatca agcagatctg
     1501 ggaccgcgcc tacgagcgcc tgctcaaggg gaacgtcggc gcccgcgggg agatccaggc
     1561 cctcgatcgc gagatgaagc tgcggacccg caaggactac ctcgaatggg aggcggtcca
     1621 ctacgccgtg ctgggccatc ccgacgacga cgactggggc cacccggtga cggcgttcac
     1681 ccccgacccc gaggagcggt gccggacgcg ggcgatcgcc tacaagacgg ccctccgcgc
     1741 ctttctcgac cagatcgagc gcagcgacct cgccaacatc cgtccccacc tcgacgcctg
     1801 gcggccgggg gtgctcgtgc cgtgccgcga ggacgggacc ttcgagacgc cgatcgcgac
     1861 gggggagctg ccgctctatc tcgcggcgag gaaggggggc tagccggtgc tggacgacgg
     1921 gatgcgccgg acctcggccc aggcccgcgg cgcgctcagg gcgccggcca gctcctccca
     1981 gagaaggggg agcggcagct cgtggaggcc cagcgccccc cgcgccaccc ggctggcgag
     2041 ccagggagcc cagcgcagca gcgcgtaggc ggcgacggcg ccgcgcgtcc gggcgtcccc
     2101 ctcgcgccag cgcttcatca gatagacgcc gaaggagcgg ccgttgtcgc ggagctgccg
     2161 gcggagctgc ggcatctccc ggcggtgatg gtgccgcacc agggccgccg gctcgtagcg
     2221 caggacgcag cccgcccgca gcaggcgatg gaagatgtcg aggtcgccgg cgccgtgggc
     2281 cggcgtgccc aggtccagcg ccgggtcgaa gccgctgacc accgcaagcg cggcgcgccg
     2341 gaacgccatg ttcgccccca cgcccacgga ctggacggcg atccgctgga aggcgccgag
     2401 gcggtcccag cgcagctccc gcgccgtggc ccccttgtcc atgccgccgc catagctctc
     2461 gaacaggcgc tgcgccgggg tctcgaccgc ggccggcagg acgcggccgg tgacgccggc
     2521 gacccgtggg tcggcgaagg cggcggccag gccggcgagc cagccggggt ccggctcgca
     2581 gtcgtcgtcg gtgtaggcca cgatgtcgtg ccgggcctcg gcggcgccgc ggttgcgcgc
     2641 ccggtccagc cccggccgct cctccagcac gtagcggcgc ccgctcgcgg ccacgatccc
     2701 cttcgccgcc gaggccgtgc cgccgcgcgg cgcgttgtcc accaccagca cctcgaacgg
     2761 cgcgtagtcg agccgggcca gggcggccag gcagcgg
//
LOCUS       DASPJI010000887        14284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_169816, whole genome shotgun sequence.
ACCESSION   DASPJI010000887 DASPJI010000000
VERSION     DASPJI010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 14284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14284)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14284
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169816"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1765)
                     /locus_tag="VEL74_25030"
     CDS             complement(<1..1765)
                     /locus_tag="VEL74_25030"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HYG65869.1"
                     /translation="MARLTDPGAAVKTLHWGRNYLYVSRLDTVAGPVDVVVKQFRHER
                     AKDRLRRRLKGSKAAKSWRVARALLEAGILTPEPVMLLESTAESGPAFYVCRHVTGVI
                     EARYFLRALNAGREREEFPQIDARGFLQEVGRMARRLHDAGFWHRDLSVGNVLIEPGP
                     QGIPSALYLLDLNRTRMGRRLTLSERARDLSRLAIHRPEHQELFLASYQGGPPGGGLK
                     TLYLAAHHGFLLKNRSKQRLRGGLRGLTGTVRGLFVPRVAHPHIPDAPAGAGARDQVV
                     WDHLSDQPHQHAGRLAKLRVRLTDAGHHAEEAAAVAGALPRIWSRYRELKADLNKQPV
                     PWDGAGLCVRPWPEAPGEVLRMVDELGVRHILLRLHLWDDDHGAEEELARDLHGRGCE
                     MTFALPQNRELVKDPARWRRAIEEIAPRFVPYGTRFQVGQAINRSKWGIWNLAEYVEL
                     VRSASEVLRRHPGVEILGPSVIDFEYHLTAAALNLRRPGLRFDAVASLLYVDRRGAPE
                     NRQAGFDTVDKVVLLKAIADTGRNSGRNSAGRSWITEVNWPLREGPHSPAGRSVSVDE
                     ESHADYLARYYLLALGTGMVER"
     gene            complement(1851..3347)
                     /locus_tag="VEL74_25035"
     CDS             complement(1851..3347)
                     /locus_tag="VEL74_25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HYG65870.1"
                     /translation="MTLKMLIESDPHQTRIAVLEDDRLTEIFVERHRHRGMVGNVYKG
                     RVTRVLPGMQAAFVDVGLERDAFLYVSDVASDVEAMEELELEEGRPEEPPSHHAAPSI
                     DDLLKQGQEIIVQVVKDPLPNKGARISTHVTLPGRYLVLLPTVRHFGVSRRIEDEAER
                     ERLLGAIGQLPTNSGGLIVRTVGEGRGVEEFESDLTYLTRLWERIRQRAGKVSAPTLL
                     HQDLDLALRVVRDLLHSDFAVLWVDGEETYERIVEFLDQVQPGLVSKVKLFRQRTSLF
                     EQFGLEEQIEAALKSKVWLKSGGYIVINPTEALVAIDVNTGRFVGQSNLEDTVLQTNL
                     EAVQEIVRQIRLRDLGGIIVLDLIDMVEQEHRERVFARLDIELRKDRAKTKMLNISEF
                     GLVEITRKRSRSNLERLLTQPCPYCGGHGRIRSVATICLNLRKELLRLRGRWAASELL
                     LRVHPEISRALQQEERAILDELERSLGLKILLQGDPELHQERFDVVEV"
     gene            complement(3421..4563)
                     /gene="rodA"
                     /locus_tag="VEL74_25040"
     CDS             complement(3421..4563)
                     /gene="rodA"
                     /locus_tag="VEL74_25040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HYG65871.1"
                     /translation="MPSFSKSISSIQPLAEEKPRLRHLSSLDGGLLLSVLLLAAAGLA
                     TVHSASAEMPIDYLPRQAAWVGVGLVLMLVVMSIDYHVLLDLSLVLYLLGIALLILVL
                     FMGEVRGGAANWLQIGPVQFQPSEFAKLATALFLARYLSGLNRRVLDLQQIAIAAAIV
                     GLPIVLVAIEPDMGGAAMYTPLLAGTLLVAGVRLRLIVIASLIVLILGVAVWSFGMQG
                     YQRQRVITFLQPESDPLGAGYQVRQSKIAVGSGELMGKGYMQGTQSQLRFLPARHTDF
                     ILAVLAEEWGFLGVCTVLGLYVLYIGSAARIAMRARDRAGIILVVGLVSIACFHVLYN
                     SAMVVGFLPITGIPLPFLSYGGSFTMINFMAAGIMLGVDMRRYVNR"
     gene            complement(4517..6316)
                     /gene="mrdA"
                     /locus_tag="VEL74_25045"
     CDS             complement(4517..6316)
                     /gene="mrdA"
                     /locus_tag="VEL74_25045"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HYG65872.1"
                     /translation="MLVVREHREDLILRIRVLTRVVTVLLVLIGAGFWFVQIVQGDYY
                     RELAENNRLRKLPIKAPRGLIYDREGRLLVENIPSYNLMFDRSRSPDAAASVRFASQV
                     LGKPVAELLAVLEGYRAIPEFKPVLLAENLTLSEVARFGVQGLEYPEFEVEVQHLRLY
                     RHREQTAHVIGYLGEVTQDEIEDSEGAYQPGDMVGKKGIEQIYDQQLRGRDGERVVVV
                     DSRGELLQEYGTTPAQPGENLTLSLDLELQQEAARWLEGPEKVGAIVAMDPRNGELLA
                     LVSSPSFNPNLFARRLQKGEWKALLEAPHNPLQNRVLQNTHSPGSVFKIVMAVAGLTE
                     GVVNEQTRFHCGGGASFYGRFFRCWRKGGHGSVDVRRAMMHSCDVYFYNVGQRLGIER
                     IAHYSRLFGLGSVTGIELHGEKKGLVPDEAWSQKVRKHKWYPGETISVSIGQGPVLMT
                     PLQMASMMAIVANGGTKVVPHLIKGGETKTEHVALDPAALRAAREGLWQVVNEPGGTA
                     YWSARVPGIDMAGKTGTVQVIAQSARTDAKALPFKYRDHAWFASFAPLEDPRLVVIVF
                     AEHGGSGSRMAAPIAQALHAKFFKVDLQHPAAR"
     gene            complement(6349..6864)
                     /gene="mreD"
                     /locus_tag="VEL74_25050"
     CDS             complement(6349..6864)
                     /gene="mreD"
                     /locus_tag="VEL74_25050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HYG65873.1"
                     /translation="MPVLKTLAAIAVAVLVHLAGVRMFPGFAQVVDVFLVVVVLHALE
                     GNSLSGMLVGLLVGLLHDSLTSAPFGLFGFADTIIGYGTARLAQRLVIQRASGVFAVV
                     SFAAVLQQAVLVTLAFLLLPDSSLPDPVWAAVKAGVCGLIGLAAHIAGRRWRRTAESR
                     RRSRMSRLRLG"
     gene            complement(6851..7699)
                     /gene="mreC"
                     /locus_tag="VEL74_25055"
     CDS             complement(6851..7699)
                     /gene="mreC"
                     /locus_tag="VEL74_25055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HYG65874.1"
                     /translation="MEERRTGWLLVVLLVGQLVFLAIQGARGSSSDTFLEAWGLRLLG
                     PAARTVTTIPESVSGAREGLRLRGSLQEENRRLRREVEELRLQLLQLTDVEQEMRRLG
                     DAVRYSAPPGGRIRAVDIVYADHVSWLRTLLLYTGDEPAREGQPVLAPQGLVGRVVTV
                     AGPYAKVQIITDRAAGVGAMVLRTRRQGVVRGSGQGEGLRLDYVPLQADVRPGDQVLT
                     AGIDGIYPRGIPIGTVVEVTQEGQLFHGIEVAPAVDLGSLDQVYLLDYTPPPPRVREA
                     LPGARP"
     gene            complement(7739..8764)
                     /locus_tag="VEL74_25060"
     CDS             complement(7739..8764)
                     /locus_tag="VEL74_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HYG65875.1"
                     /translation="MPLGSFLRYFSKDLAIDLGTANTLVFASNQGIVVREPSVVVINK
                     LTNRIEAVGSEAKEMLGRTPGNIESIRPMKDGVIADFEVTEKMLEYFIKKAHGRKMYV
                     HPRIVIGVPSEITQVEKRAVRDSAMRAGASEVFLVEQAMMAAIGAGLPITEPSGNMIV
                     DIGGGTTDVAVISLAGTVYSRSVRIAGNEMDVALIQYLKRKYNLLIGERTAEQIKIEL
                     GSAYPLKEEIRMEIKGRDLVEGVPKTLTISDEEIREALAEPVATIVEAVRMALERTPP
                     ELSADIMDKGIVLSGGGALLRNLDQRLRDETGLPVVLAEDPLASVVLGTGRVLSDIDL
                     LRKVSIR"
     gene            complement(8862..10298)
                     /locus_tag="VEL74_25065"
     CDS             complement(8862..10298)
                     /locus_tag="VEL74_25065"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HYG65876.1"
                     /translation="MADRKAARPTKDWYSVSVDTLRGWALLLTIIVLLVASYFGYRLW
                     ERRSLEREAAQVLEETADLFQRLQNEKRAASFGKELNAAHESFDQARNRFLAKEFRQS
                     LSDGLRSRGVLLHILDSLALPSGASQANFLSVQGEVEYRRGEGGEWEEARSRLPLRPG
                     DYVRTSGSGSAEIMFTDGTLYTVRPNTQFIVSAGRSAPGRPAEQTIQMEYGWVDLNTT
                     QNSSQVRTPGAVAEVRKDSEAFVAFDKDSNRGRFGAYRGGMELAAKGGMKQEVNELQQ
                     VVQTGDLLSAPSALPGRPELLEPAHQLSLDMNRQRTLVLAWEPVTGASRYKLEVSRNN
                     LFVDNVVQDPSRAKTRATLSLRGEGTFLWRVAALGGDGMQGPWSPAREFRVSALSAES
                     GERDKTPPKLDLDDVKSYGSIFIVDGRSEPGSRIEVNGEQVKVAADGSFTKTVQLNKE
                     GWSFIEIKAYDTSGNVAVQNRRVFVENP"
     gene            10396..12339
                     /locus_tag="VEL74_25070"
     CDS             10396..12339
                     /locus_tag="VEL74_25070"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HYG65877.1"
                     /translation="MLKVFRDNLKHLSWILWAVIALFVLLVFVDYGSLTQPGGAAGTT
                     AATVGGQTVSMQEFERSYRALEAQYQQSFGEQFTPELARQMRLPLQALDQLINDKILL
                     EEAEQMGLRVSDAEVQQAILEVDAFKDEKGQFIGQELYSQVLQSNGFTVAEFERQVRE
                     DILRDKLRNALLASAYVSEAELQEAYRAQVERAKIRYVLLPGSRFAAETQVTPQEMQS
                     YYQAHKEEFRLPEQREAAYLLVENEKLAGQVKIDDAELRKFYDDNKAEFTQEEQVRAR
                     HILVMVNDQQSDAVAKGKIEQAQKRLQAGEDFAKVAAEVSEDTASKAQGGDLSYFGRG
                     SMVKEFEDAAFGAQPGQLVGPVKSTYGYHLIEVTDRRPGGSTPFEQAREQIRARLAAE
                     RVQTLAQAKATELARQLQEDTPANAKELQAVAEKNPGVSFAETGRFGRQEPVSGLGFS
                     PAFTNAAFELEKGKVSEPVQVQRGWAIAYLTAVHEPRVPELKDVEARVRMAVTADKQK
                     KVAVERLQAARAGGKTLDQVAAELGIQVQETAEFGSQGAIPGLGLNPQLAKAALSMKQ
                     GETGGPIATSQGAVLFQVTERKAFDPAQFATAKEQTRSTLQQEKAARLLASLVEQRRK
                     ELGVEYDGQLLQTFGVEGAGQPS"
     gene            12408..12995
                     /gene="ruvA"
                     /locus_tag="VEL74_25075"
     CDS             12408..12995
                     /gene="ruvA"
                     /locus_tag="VEL74_25075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HYG65878.1"
                     /translation="MIGYLQGHLLKATPEQLLLDVQGVGYEVHIPLSTYYEIERRPGE
                     PIRLFIHTHMREDGIALFGFWTEREKALFERLIGVGGIGPRLARVVLSGMAPDDLLAA
                     IAAGDVARLSTIPGIGKKTAERMVLELKDKMRELAAELPERAMPADQDVVSALVNLGY
                     KAAQAERAVADARRERPDAAFHDLLRASLQRLSRG"
     gene            complement(13010..13897)
                     /locus_tag="VEL74_25080"
     CDS             complement(13010..13897)
                     /locus_tag="VEL74_25080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65879.1"
                     /translation="MHARVRGPVWWRLSCVLACSAALLPAVVHAQGTSTQTHPTHTFS
                     TPGVKEVKLTVCKPSIMLGEQCSTVTKTVTVLDPRPAVTSTSATPAGAEAGQVVRLTG
                     AATGQPPLSLSWRVSLAGIPILELPGSTIWWDTRGLVPGLYSLTFQAQNASGSASSQV
                     PVSLGATQAADFYTVAPCRVYDSRIATPLASGAEARTFPVTDACEIPANARAVVANVT
                     AVSPTGTGHVTVFPGNYPAPATSSINFNAGTTRANRAILQLSTDGTGTLAATASVANG
                     GSVHLLVDVTGYFAAPPAT"
     gene            14022..>14284
                     /locus_tag="VEL74_25085"
     CDS             14022..>14284
                     /locus_tag="VEL74_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65880.1"
                     /translation="MIMFIGHFAVGLGAKRIAPSVSLGTLFLAGQLADLLWPTFVLLG
                     LERVDIAPGITAVTPLDFVRYPYSHSLVALLGWAALLALGYAVA"
BASE COUNT         2186 a         4917 c         5085 g         2096 t
ORIGIN      
        1 ccctctccac cattcccgtc cccagggcca gcaggtaata gcgcgccaga tagtcggcgt
       61 gcgactcctc gtccaccgag acggagcggc cggcggggga gtgcggcccc tcgcgcaagg
      121 gccagttcac ctcggtgatc cacgagcggc cggcgctgtt cctcccgctg ttcctcccgg
      181 tatcggcgat cgccttgaga agcaccacct tgtccaccgt gtcgaagccc gcctgccggt
      241 tctcgggggc gccgcggcgg tcgacgtaga ggaggctcgc gacggcgtcg aagcgcaggc
      301 cggggcggcg gaggttgagc gccgccgcgg tcaggtgata ttcgaagtcg atcaccgacg
      361 ggcccaggat ctccaccccg gggtgccggc gcagcacctc cgaggccgag cgcaccagct
      421 ccacgtactc ggccaggttc cagatccccc acttgctgcg gttgatcgcc tgacccacct
      481 ggaagcgcgt gccgtagggg acgaagcgcg gcgcgatctc ctcgatggcg cggcgccagc
      541 gggcggggtc cttcaccagc tcgcggttct gcggcagggc gaaggtcatc tcgcagcctc
      601 tcccatggag atcccgcgcc agctcctcct cggcgccgtg gtcgtcgtcc cagagatgga
      661 gccggaggag gatgtgacgg acccccagct cgtccaccat gcggaggacc tcaccgggtg
      721 cctcgggcca ggggcggacg cagagcccgg caccgtccca gggcaccggc tgcttgttca
      781 ggtcggcctt cagctcgcgg tagcgcgacc agatcctggg gagggcgccg gccacggccg
      841 ccgcctcctc ggcgtggtgc ccggcgtcgg tcagccgcac ccgcagcttc gcgaggcgcc
      901 ccgcgtgctg gtgcggctgg tccgagaggt ggtcccagac cacctggtcg cgcgctccgg
      961 cccccgccgg cgcgtccggg atgtgcggat gcgccacccg cggcacgaag aggccgcgca
     1021 ccgtgccggt gaggccgcgc aggccgccac gcagccgctg cttgctccgg ttcttgagca
     1081 ggaacccgtg gtgggcggcg aggtagagcg tcttgagccc gccgccgggc ggccccccct
     1141 ggtagctcgc caggaacagc tcctgatgct ccggccggtg gatggccagg cgcgacaggt
     1201 cgcgcgcccg ctccgacagg gtgaggcgcc tccccatgcg ggtgcggttg aggtccagga
     1261 gatagagggc ggacgggatc ccctgggggc ccggctcgat caagacgttg cccaccgaca
     1321 ggtcgcggtg ccagaagccg gcgtcgtgga gccgccgcgc catccggccg acctcctgga
     1381 ggaagccgcg ggcgtcgatt tgcgggaact cctcgcgctc ccggccggcg ttcagggcgc
     1441 gcaggaagta gcgcgcctcg atcaccccgg tcacgtgacg gcagacgtag aaggcggggc
     1501 cggactcggc ggtggactcg aggagcatca ccggctcggg ggtgaggatg ccggcttcga
     1561 ggagcgcccg cgccacccgc cagctcttcg ccgccttgct ccccttgagg cgccgccgca
     1621 gccggtcctt ggcccgctcg tgccggaact gcttgacgac cacgtcgact gggccggcga
     1681 ccgtgtcgag ccgcgacaca tagagatagt tgcggcccca gtgcagcgtc ttgaccgccg
     1741 ctccggggtc ggtgaggcgg gccaccgcgg cggcgaggtc ggcgggtcgg aaggccgggt
     1801 ccagctcccc ggaccagccc gggagggaga aggtctcggc ggggtcgatc tcagacctcc
     1861 acgacgtcga agcgctcttg atggagctcg gggtccccct ggagcaggat cttcagcccc
     1921 aatgaccgct ccagctcgtc gaggatcgcc cgctcctcct gctgcagggc gcgcgagatc
     1981 tcggggtgga cgcgcagcag cagctccgag gcggcccagc ggccgcgcag gcgcagcagc
     2041 tccttgcgca ggttgaggca gatcgtggcc accgagcgga tgcggccgtg cccgccgcag
     2101 taagggcaag gctgggtgag cagccgctcg aggttcgacc ggctgcgctt gcgggtgatc
     2161 tccaccaggc cgaactcgga gatgttgagc atcttggtct tcgcccggtc cttgcgcagc
     2221 tcgatatcga ggcgggcgaa gacccgctcg cggtgctcct gctcgaccat gtcgatgagg
     2281 tcgaggacga tgatgccgcc gaggtcgcgc aggcggatct ggcgcacgat ctcctgcacc
     2341 gcctcgaggt tggtctggag caccgtgtcc tcgaggttgc tctggccgac gaagcgcccg
     2401 gtgttgacgt cgatcgccac cagcgcctcg gtggggttga tgacgatgta gccgccggac
     2461 ttgagccaga ccttgctctt gagcgccgcc tcgatctgct cttcaaggcc gaactgctcg
     2521 aagagcgacg tccgctggcg gaacagcttg accttgctga ctagcccggg ctgcacctgg
     2581 tcgaggaact cgacgatgcg ctcgtaggtc tcctcgccgt ccacccagag gacggcgaag
     2641 tcggagtgca ggaggtcgcg caccacccgg agggcgaggt cgaggtcctg gtgcagcagg
     2701 gtgggggcgg agaccttgcc ggcgcgctgg cggatgcgct cccagaggcg cgtcaggtag
     2761 gtgaggtcgc tctcgaactc ctcgacgccg cgcccctcgc cgacggtgcg cacgatcagc
     2821 ccgccgctgt tggtggggag ctggccgatg gcgccgagca ggcgctcgcg ctcggcctcg
     2881 tcctcgatgc ggcgcgagac gccgaagtgg cgcacggtgg ggagcagcac caggtagcgg
     2941 ccgggcagcg tcacgtgggt gctgatccgc gcccccttgt tgggcagcgg gtccttgacc
     3001 acctggacga tgatctcctg cccctgcttg aggaggtcgt cgatggacgg ggcggcgtgg
     3061 tgggacggcg gctcctccgg ccgcccctcc tcgagctcca gctcctccat cgcctcgacg
     3121 tccgaggcca cgtcgctgac gtagaggaag gcgtcgcgct ccaggccgac gtcgacgaac
     3181 gccgcctgca tgccggggag cacccgcgtc acccgcccct tgtagacgtt gcccaccatc
     3241 ccccggtgac ggtggcgctc gacgaagatc tccgtcaggc ggtcgtcctc gaggacggcg
     3301 atccgcgtct ggtgcggatc gctctcgatg agcatcttca atgtcatggc tcgctccgcc
     3361 cggcgcaggc gactggcagc gtagcgcaca cgaaggagcc gagcagggcg gagcgggcca
     3421 tcatcggttc acgtagcggc gcatgtccac tcccagcatg atccccgcgg ccatgaagtt
     3481 gatcatggtg aaggagccgc cataggagag gaagggcagg gggatgccgg tgatgggcag
     3541 gaagcccacc accatggcgg agttgtagag cacgtggaag caggcgatgg acaccagccc
     3601 caccaccagg atgatgccgg cgcggtcgcg ggcccgcatg gcgatgcggg cggcgcttcc
     3661 tatatagagg acgtagaggc cgagcaccgt gcagacgccg aggaagcccc actcctcggc
     3721 cagcaccgcg aggatgaagt cggtgtggcg cgccggcagg aagcggagct ggctctgggt
     3781 gccctgcatg tagcccttgc ccatcagctc cccggagccc accgcgatct tgctctggcg
     3841 cacctggtag ccggccccca gcggatcgct ctccggctgc aggaaggtga tgacgcgctg
     3901 gcgctggtag ccctgcatgc cgaagctcca caccgccacg cccaggatga ggacgatcag
     3961 cgacgcgatg acgatcagcc gcagccgcac tccggccacc agcagcgtgc cggcgagcag
     4021 cggcgtgtac atggcggcgc cgcccatgtc cggctcgatg gcgaccagca cgatgggcag
     4081 gccgacgatc gcggcggcga tggcgatctg ctggaggtcg agcacccggc ggttcagccc
     4141 cgacaggtag cgggccagga acagcgccgt ggcgagcttg gcgaactccg acggctggaa
     4201 ctggacgggg ccgatctgca gccagttggc cgcgcctcct ctcacctcgc ccatgaagag
     4261 caccaggatc agcagggcga tgcccagcag gtagagcacc agcgagaggt cgaggagcac
     4321 gtggtagtcg atgctcatca ccaccagcat caggacgagc cccaccccca cccacgcggc
     4381 ctggcgcggc aggtagtcga tgggcatctc ggcgctggcg ctgtgcaccg tggcgaggcc
     4441 ggcggcggcg aggagcagga cggacagcag gagcccgccg tcgagcgacg acaggtgccg
     4501 cagccgcggc ttctcctcag cgagcggctg gatgctggag atcgactttg aaaaacttgg
     4561 catgcagggc ctgcgcaatc ggtgccgcca tcctcgaccc cgagccgcca tgctcggcga
     4621 agacgatcac caccaggcgg gggtcttcga gcggggcgaa cgaggcgaac caggcgtggt
     4681 cacggtattt gaaagggagg gccttggcgt cggtgcgggc cgactgggcg atgacctgga
     4741 ccgtgccggt cttgcccgcc atgtcgatgc ccggcacccg cgccgaccag taggcggtgc
     4801 cgcccggctc gttgaccacc tgccagaggc cttcgcgcgc cgcgcgcaac gccgccgggt
     4861 cgagggccac gtgctcggtt ttggtctcgc cgcccttgat cagatggggg acgaccttgg
     4921 tgccgccgtt ggcgacgatg gccatcatcg acgccatctg cagcggcgtc atcagcaccg
     4981 gcccctggcc gatggagacg gagatggtct cgccggggta ccacttgtgc ttgcgcactt
     5041 tctggctcca cgcctcgtcc ggcaccagcc ccttcttctc gccgtgcagc tcgatgccgg
     5101 tgaccgagcc gaggccgaac aggcgcgagt agtgggcgat gcgctcgatc cccagccgct
     5161 ggccgacgtt gtagaagtag acgtcgcagg agtgcatcat cgcccgccgc acgtccaccg
     5221 agccgtgccc ccccttgcgc cagcaccgga agaaacggcc gtagaagctg gcgccgccgc
     5281 cgcagtggaa gcgggtctgc tcgttgacca ccccctcggt caggccggcg accgccatga
     5341 cgatcttgaa caccgagccc ggcgagtggg tgttctggag cacccggttc tgcagcgggt
     5401 tgtgcggcgc ctcgagcagc gccttccact cccccttctg gaggcggcgg gcgaacaggt
     5461 tggggttgaa ggagggcgac gagaccagcg ccagcagctc gccgttgcgc gggtccatgg
     5521 cgacgatggc gccgaccttc tccgggccct cgagccagcg cgccgcctcc tgctggagct
     5581 cgaggtcgag ggagagggtg aggttctcgc ccggctgggc gggggtggtg ccgtactcct
     5641 ggagcagctc gccgcggctg tcgaccacca ccacccgctc gccgtcgcgg ccgcggagct
     5701 gctggtcgta gatctgctcg atccccttct tgcccaccat gtcgccgggc tggtaggcgc
     5761 cctcggagtc ctcgatctcg tcctgcgtca cctcgcccag gtagccgatg acgtgggcgg
     5821 tctgctcgcg gtgccggtag aggcgcaggt gctggacctc gacctcgaac tccgggtact
     5881 cgagcccctg gacgccgaag cgcgccacct cggagagggt caggttctcg gcgagcagca
     5941 ccggcttgaa ctcggggatg gcgcggtagc cctcgagcac cgccagcagc tcggccaccg
     6001 gcttgcccag cacctgggag gcgaagcgga cgctcgccgc cgcgtcgggg ctgcggctgc
     6061 ggtcgaacat caggttgtag ctcgggatgt tctcgaccag cagccggcct tcgcggtcgt
     6121 agatcaggcc gcggggcgcc ttgatgggga gcttgcgcag ccggttgttc tcggccagct
     6181 cgcggtagta gtccccctgg acgatctgga cgaaccagaa gcccgccccg atcagcacca
     6241 gcaggacggt gaccacccgg gtgagcaccc ggatgcgcag gatcagatct tcccgatgtt
     6301 cgcgtacgac cagcatgggg tgtccttccg gtatttcagc cgctgtcctc agccgagacg
     6361 cagacggctc atgcggctgc ggcgccggct ctcggcggtg cggcgccagc gccggccggc
     6421 gatgtgggcg gcgaggccga tcagcccgca gacgcccgcc ttgaccgccg cccagaccgg
     6481 gtccggcagc gacgagtcgg gcagcagcag gaaggccagg gtgaccagga ccgcctgctg
     6541 cagcacggcg gcaaagctca ccaccgcgaa gacgccggag gcccgctgga tgaccaggcg
     6601 ctgggccagc cgcgccgtgc cgtagccgat gatggtatca gcgaagccga agaggccgaa
     6661 gggggcgctg gtcagggagt cgtggagcag cccgaccagc aggccgacga gcatccccga
     6721 cagcgagttg ccctccaggg cgtggagcac cacgacgacc agaaagacgt cgaccacctg
     6781 ggcgaagccg gggaacatcc gcacccccgc caggtggacg aggacggcga cggcgatggc
     6841 ggcgagcgtc ttaaggacgg gcacccggca gggcctcccg cacccgcggc ggcgggggcg
     6901 tgtagtcgag gagataaact tggtcgaggg agccgaggtc gacggccggc gccacctcga
     6961 tgccgtggaa gagctggccc tcctgggtca cctccaccac cgtgccgatc gggatgccgc
     7021 gcggatagat gccgtcgatg ccggcggtca gcacctggtc gccggggcgg acgtcggcct
     7081 ggagcggcac gtagtcgagg cggagcccct cgccctggcc gctgccgcgc accaccccct
     7141 ggcggcgggt gcgcaggacc atcgccccga cgccggcggc gcgatcggtg atgatctgca
     7201 ccttggcata ggggccggcc acggtcacca cccgcccgac caggccctga ggggcgagca
     7261 ccggctgccc ctcgcgggcg ggctcgtcgc cggtgtagag gagcagggtg cgcagccagg
     7321 agacgtggtc ggcatagacg atgtccacgg cgcggatgcg gccgcccggc ggcgcggagt
     7381 agcggacggc gtcgcccagc cgccgcatct cctgctcgac gtcggtgagc tggagcagtt
     7441 gcagccgcag ctcctcgacc tcgcggcgca gccgccggtt ctcctcctgc agggagccgc
     7501 gcagccgcag cccctcgcgg gcgccggaga cgctctcggg gatggtggtc accgtgcgcg
     7561 ccgccgggcc gaggaggcgg agcccccagg cttcgagaaa ggtgtcggag ctggagccgc
     7621 gcgccccctg gatcgccagg aagacgagct ggcccaccag caggaccacc aggagccagc
     7681 ccgtgcggcg ctcttccacg gcttcgctcc gtgccacttc aaccgcgcca gacccgggtt
     7741 accggatgga gaccttgcgc aggaggtcga tgtccgacag cacgcggccg gtgcccagca
     7801 ccaccgaggc gagcgggtcc tcggcgagga cgaccggcag gccggtctcg tcgcgcagcc
     7861 gctggtcgag gttgcgcagc agggcgccgc cgccggagag cacgatcccc ttgtccatga
     7921 tgtcggccga cagctccggc ggggtgcgct cgagggccat gcgcaccgcc tcgacgatgg
     7981 tggcgaccgg ctccgccagc gcctcgcgga tctcctcgtc ggagatggtc agggtcttgg
     8041 ggacgccttc gacgagatcg cgccccttga tctccatccg gatctcttcc ttgagcggat
     8101 aggccgagcc gagctcgatc ttgatctgct cggcggtgcg ctcgccgatg agcaggttgt
     8161 acttgcgttt caggtactgg atgagcgcca cgtccatctc gttgccggcg atccgtaccg
     8221 agcggctgta caccgtgccg gcgagcgaga tgaccgccac gtccgtggtg ccgccgccga
     8281 tgtcgacgat catgttgccc gacggctcgg tgatcggcag cccggcgccg atcgccgcca
     8341 tcatcgcctg ctccaccagg aagacctccg aggcgccggc ccgcatcgcc gagtcgcgca
     8401 cggcccgctt ctccacctgg gtgatctccg acggcacgcc gatgacgatg cgcgggtgca
     8461 cgtacatctt gcgcccgtga gccttcttga tgaagtactc gagcatcttt tcggtgacct
     8521 cgaagtcggc gatgacgccg tccttcatcg gccggatgga ctcgatgttg ccgggggtgc
     8581 gccccagcat ctccttggct tcgctcccca ccgcctcgat ccggttcgtg agcttgttga
     8641 tgacgacgac ggagggctcg cgcaccacga tgccctggtt gctggcgaag accagggtgt
     8701 tggcggtgcc gagatcgatg gcgaggtctt tggagaagta gcgcagaaac gaaccgaggg
     8761 gcatcagctt tcctactttt gacgagggct ccggacaggc gagcgacgcg ggctgcagcg
     8821 gcggccggaa gcggggttca cgggtttatc gggacgacga atcagggatt ttcgacgaac
     8881 acacggcggt tctgcaccgc cacattgccg gacgtatcgt acgccttgat ctcgatgaag
     8941 ctccagcctt ccttgttgag ctggacggtc ttggtgaacg agccgtccgc cgcgaccttg
     9001 acctgctcgc cgttgacttc gatccgggat ccgggctcgc tgcgcccgtc gacgatgaaa
     9061 atactaccgt aggacttcac gtcgtcgagg tcaagcttgg gaggcgtctt gtcccgctcg
     9121 ccgctctcgg cgctcagggc ggagacgcgg aactcgcgcg ccgggctcca cggcccctgc
     9181 atcccgtccc ctcccagagc ggcgacccgc cacaggaagg tcccctcgcc gcgcagggac
     9241 agcgtcgccc gtgtcttcgc ccggctcggg tcctggacga cattatcgac gaaaaggttg
     9301 ttgcgcgaca cctccagctt gtaacggctg gcgccggtga ccggctccca ggcgagcacg
     9361 agggtgcgct gccggttcat gtcgaggctg agctggtgcg cgggctccag gagctcgggg
     9421 cggccgggca gggccgaggg ggcggagagc aggtcgccgg tctgcaccac ctgctgcagc
     9481 tcgttgacct cctgcttcat cccccccttg gcggccagct ccatgccgcc gcggtaggcg
     9541 ccgaagcggc cgcggttgga gtccttgtcg aaggcgacga aggcctccga gtccttgcgc
     9601 acctcggcca ccgcgcccgg cgtccgcacc tggctgctgt tctgggtggt gttgaggtcg
     9661 acccagccgt actccatctg gatggtctgc tcagccggcc ggcccggcgc cgagcgcccg
     9721 gccgagacga tgaactgggt gttggggcgc acggtgtaga gggtgccgtc ggtgaacatg
     9781 atctcggccg agccgctgcc cgacgtccgc acgtagtcgc ccgggcgcag ggggaggcgg
     9841 ctgcgcgcct cctcccattc gcccccctcg ccgcgccggt actcgacctc cccctggacg
     9901 gagaggaagt tggcctggct ggcgcccgac ggcagggcca gggagtcgag gatgtggagc
     9961 agcacgccgc ggctgcggag gccgtcgctc agcgattgcc ggaattcctt cgcgaggaac
    10021 cggttgcggg cttggtcgaa gctctcgtgg gcggcgttga gctccttgcc gaagctggcg
    10081 gcccgcttct cgttctgcaa cctctggaac aggtccgccg tctcctccag cacctgggcg
    10141 gcctcgcgct ccagggagcg ccgttcccag agccgatagc cgaaatagct cgccaccagc
    10201 aacacgatga tcgtcaacag cagggcccag ccccgcagtg tgtccaccga tacgctgtac
    10261 cagtccttgg tcgggcgggc tgccttccgg tcggccacct ctcaactccc tttcatgtag
    10321 gaaaccgaat atacaccaca aaaccccgcc cgtggtaaag ttcgccgttc cccagggagc
    10381 gaaaggagtt cgttcatgct caaggtcttc cgcgacaatt tgaaacacct cagctggatc
    10441 ctctgggcgg tcatcgccct gttcgtgctt ctggtcttcg tcgactacgg ctccctcacg
    10501 cagccgggcg gcgccgccgg caccaccgct gccacggtgg gcggccagac ggtgtcgatg
    10561 caggagttcg agcgctccta ccgggcgctt gaggcgcagt accagcagag cttcggcgag
    10621 cagttcaccc ccgagctggc gcgccagatg cggctgccgc tccaggcgct cgaccagctc
    10681 atcaacgaca agatcctcct cgaagaggcg gagcagatgg gcctgcgggt gagcgacgcc
    10741 gaggtccagc aggcgatcct ggaggtcgac gccttcaagg acgagaaggg gcagttcatc
    10801 ggccaggagc tctacagcca ggtgctgcag tccaacggct tcacggtcgc cgagttcgag
    10861 cgccaggtgc gcgaggacat cctgcgcgac aagctgcgca acgccctgct ggcgagcgcc
    10921 tacgtcagcg aggccgagct gcaggaggcc taccgcgcgc aggtggagcg ggccaagatc
    10981 cgctacgtgc tgctgcccgg cagccgcttc gccgccgaga cccaggtgac gccgcaggag
    11041 atgcagtcct actaccaggc gcacaaggag gagttccggc tccccgagca gcgcgaggcg
    11101 gcctacctgc tggtcgagaa cgagaagctc gccggccagg tgaagatcga cgacgccgag
    11161 ctgcgcaagt tctacgacga caacaaggcc gagttcaccc aggaggagca ggtgcgcgcc
    11221 cgccacatcc tggtgatggt caacgaccag cagagcgacg cggtggccaa gggcaagatc
    11281 gagcaggccc agaagcggct ccaggcgggc gaggacttcg ccaaggtggc cgccgaggtc
    11341 tccgaggaca ccgcctccaa ggcgcagggg ggcgacctca gctacttcgg gcgcggcagc
    11401 atggtcaagg agttcgagga cgccgccttc ggcgcccagc ccgggcagct cgtcggcccc
    11461 gtcaagtcca cctacggcta tcacctgatc gaggtgaccg accgccggcc cggcggctcg
    11521 acgccgttcg agcaggcgcg cgagcagatc cgcgcccgcc tcgccgccga gcgggtgcag
    11581 accctggccc aggccaaggc gaccgagctc gcccgccagc tccaggagga cacgccggcc
    11641 aacgccaagg agctccaggc cgtcgccgag aagaacccgg gcgtgagctt cgccgaaacc
    11701 ggccgcttcg gccgccagga gccggtgagc ggcctgggct tctccccggc cttcaccaac
    11761 gccgccttcg agctggagaa gggcaaggtc tcggagccgg tgcaggtgca gcgcggctgg
    11821 gcgatcgcct acctgaccgc ggtccacgag ccgcgcgtcc ccgagctcaa ggacgtggag
    11881 gcccgggtgc gcatggcggt gaccgccgac aagcagaaga aggtggcggt cgagcggctg
    11941 caggcagccc gcgccggcgg caagaccctg gaccaggtgg ccgcggagct cggcatccag
    12001 gtgcaggaga ccgccgagtt cggcagccag ggggcgatcc ccggcctcgg cctcaacccg
    12061 cagctcgcca aggcggccct gtcgatgaag cagggggaga ccggcggccc gatcgccacc
    12121 tcccagggcg ccgtgctctt ccaggtgacg gagcgcaagg ccttcgaccc cgcccagttc
    12181 gccaccgcca aggagcagac ccgctcgacg ctgcagcagg agaaggcggc gcggctgctc
    12241 gcctccctgg tggagcagcg gcgcaaggag ctgggggtcg agtacgacgg ccaactcctc
    12301 cagaccttcg gcgtcgaagg agccgggcag ccgagctgag cggacggcca gaacggcggc
    12361 gcgagcctcc ccgcgccgcc ccttccttct cctatcctca taccgcgatg atcggctacc
    12421 tgcagggcca cctcctcaag gccaccccgg agcagctcct cctcgacgtg cagggggtgg
    12481 gctacgaggt tcacatcccg ctgtccacct attacgagat cgagcggcgc ccgggcgagc
    12541 ccatccgcct gttcatccac acccacatgc gggaggacgg catcgccctg ttcggcttct
    12601 ggaccgagcg ggagaaggcg ctgttcgagc ggctgatcgg cgtcggcggc atcggcccgc
    12661 gcctcgcccg ggtggtcctc tccggcatgg cgccggacga cctgctcgcc gccatcgccg
    12721 ccggtgacgt ggcgcggctc tcgaccatcc cggggatcgg caagaagacg gccgagcgga
    12781 tggtgctgga gctcaaggac aagatgcggg agctggcggc cgagctgccc gagcgggcca
    12841 tgccggccga ccaggacgtg gtctccgccc tggtcaacct cggctacaag gccgcccagg
    12901 ccgagcgggc cgtggccgac gcccggcgcg agcgtccgga cgccgccttc cacgacctgc
    12961 tgcgggcgag cctgcaacgg ctctcccgcg gctgaccgtt cctgccttcc tacgtcgccg
    13021 gcggcgcggc gaagtacccg gtcacgtcca ccagcagatg gacgctgccg ccgttggcga
    13081 cgctggcggt ggcggcgaga gtccccgtgc cgtccgtgga gagctgcagg atcgcgcggt
    13141 tggcgcgggt ggtgccggcg ttgaagttga tgctgctggt cgccggcgcc ggatagttgc
    13201 ccgggaagac cgtcacgtgg cccgtgccgg tgggagagac cgcggtgacg ttggcgacca
    13261 cggcgcgggc gttggccggg atctcacagg cgtcggtgac cgggaaggtc cgcgcctccg
    13321 cgccgctggc gagaggcgtc gcgatgcggc tgtcgtacac ccggcagggg gcgacggtgt
    13381 agaagtcggc ggcctgggtg gcgccgaggc tcaccggcac ctgggacgag gcggagccgg
    13441 acgcgttctg cgcctggaag gtgagggaat agagcccggg caccagcccc cgggtgtccc
    13501 accagatcgt ggacccgggc agctcgagga tcgggatgcc ggcgagcgac acccgccagg
    13561 acagggagag cggcggctgg ccggtggccg cgccggtgag ccgcaccacc tggccggcct
    13621 cggcgcccgc cggagtggcg gaggtcgagg tcaccgccgg ccgggggtcg agcaccgtca
    13681 ccgtcttggt caccgtggag cattgctccc ccagcatgat gctgggcttg cagacggtga
    13741 gcttgacctc cttcactccg ggggtcgaaa acgtgtgcgt cgggtgggtc tgagtagagg
    13801 taccctgcgc atggacgaca gccggcagca aggccgccga gcaggcgagg acgcaggaga
    13861 gccgccacca gaccggaccg cgcactctcg cgtgcacaga ggctccggcc atagaagagt
    13921 ccttttatat ggcgctacgc tatagatttt ttggcaaatg gtcaagcgcg taggcgccgg
    13981 gagctccggt acactctcgt ccggacgacg gctggaggtt catgatcatg ttcatcgggc
    14041 acttcgcggt ggggctcggg gcgaagcgga tcgctccgtc ggtctccctg ggcacactct
    14101 tcctggccgg gcagctcgcc gacctcctct ggccgacctt cgtgctgctc gggctcgaac
    14161 gggtcgacat cgcgcccggg atcactgccg tgacgccgct ggacttcgtg cgctacccct
    14221 actcccacag cctggtggcg ctgctcggct gggccgcgct cctcgcgctg gggtatgccg
    14281 tggc
//
LOCUS       DASPJI010000888         7550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_736369, whole genome shotgun sequence.
ACCESSION   DASPJI010000888 DASPJI010000000
VERSION     DASPJI010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736369"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VEL74_25090"
     CDS             <1..1218
                     /locus_tag="VEL74_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HYG65881.1"
                     /translation="VGYGCTTCIGNSGPLPPSISKAIEERDLVAVSVLSGNRNFEGRI
                     NSDVRANYLASPPLVVAYALAGRIDIDWQSEPVGTGKDGQPVYLKDIWPSRREVEETV
                     LKALHAQMFREQYQDVFAGDERWNGLDIPEGDTYAWDDASTYVRLPPYFTDMPETPPP
                     VTDVRGARVLGLFADSITTDHISPAGSIKAASPAGRYLQEHGVAPKDFNSYGARRGNH
                     EAMMRGTFANVRLRNRLVPEIEGGFTVHFPDGEPLSIYDAAMKYQKEGVPTVILAGKE
                     YGTGSSRDWAAKGPLLLGVRAVIAESFERIHRSNLVGMGVIPLDFPDGQNADSLGLTG
                     TEVYDIEGLAHGVETGFAGGREVTVRATPQDGSGKVIELKARVRLDTPQEVQYYRHGG
                     ILHYVLRQLLGKS"
     gene            complement(1233..1769)
                     /locus_tag="VEL74_25095"
     CDS             complement(1233..1769)
                     /locus_tag="VEL74_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HYG65882.1"
                     /translation="MSESVANLYLKDVDEQLRKLKELADRAVAQVRDEDLTAVLDPES
                     NSLAVLLQHVGGNLRSRWTDFLTTDGEKPGRDRDSEFEQGEGTTRQALLARWEEGWAC
                     LFASLAELKEEDLPRTVLIRAEPHSVVKAIDRTMTHMAYHVGQIVFLAKHFASESWKT
                     LSVPRGGTKEYNREKMGR"
     gene            1819..3312
                     /gene="gatB"
                     /locus_tag="VEL74_25100"
     CDS             1819..3312
                     /gene="gatB"
                     /locus_tag="VEL74_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HYG65883.1"
                     /translation="MSGEWEAVIGLEVHVQLLTESKMFCRCPNRFGAEPNTLVCPVCL
                     GYPGTLPVVNRHAVDLATKLALALGCEVRGTSVFARKNYFYPDLPKGYQISQFDKPLA
                     ENGALALTQHEKSVRIERLHMEEDAGKLLHEAPGGAKLPGESLVDFNRCGVPLVEIVS
                     RPDMSTPAEAQDYLQTLHQLLLYTGTSDGNMEEGSLRCDANVSVRRRGETRLGTKAEV
                     KNVNSFRNVARAIEHEIQRQIQVIEAGGQVAQETRTFDADAGVTRAMRSKEEAHDYRY
                     FPEPDLPPLVLARERIDAIRGTLPELPWDRRARFADVSGGYGLPAEAARVLTASRELA
                     DYFEAAAAALPANAKGPKGIGNWVMTEVLRVLKERKLEVGPDLESAMPPARLAALVAL
                     QDEGKISSSAAKEVFAAIWDSGEEPAAAVERLGLGQVSDSAQIERWIDEVLEENPAQV
                     AQYRAGKVQVVGFLVGQVMKRSGGRAEPKGVQQLLRQTLERAPVVPE"
     gene            3346..4002
                     /gene="ftsE"
                     /locus_tag="VEL74_25105"
                     /pseudo
     CDS             3346..4002
                     /gene="ftsE"
                     /locus_tag="VEL74_25105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
     gene            4185..5072
                     /locus_tag="VEL74_25110"
     CDS             4185..5072
                     /locus_tag="VEL74_25110"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HYG65884.1"
                     /translation="MSFLQALLYFFREALVNLLRSWKVGLLAVLTITVSLLVGGAFLL
                     MSRNLAASVGQWQKEMRVVIYLRPDTGADELRRLAAEAGRPAWVAAVEPVTAEAARDR
                     FTEIFPTLSDLVEGWEDEPLPASLEIRLGDFEPATLETWLAGWRRRPEVSMVDDDREW
                     LRQVATAVAVVRGVGLALGAGLLAAAIFTIMSVIRLTAYLHHEEISIMRLVGATEFYI
                     RGPFYAEGLLQGLLGGGLASAGLYGAYQLLHARSPGSLFTTVLAGTFLSWREIAFLIL
                     LGGGAGLLGAVASLRRESL"
     gene            complement(5486..6868)
                     /locus_tag="VEL74_25115"
     CDS             complement(5486..6868)
                     /locus_tag="VEL74_25115"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HYG65885.1"
                     /translation="MKLKYLLFIMLLSLVPSMGFAGEEANAPTVTGETGLFTLSSGDT
                     LPDGEWSFSLYYNNWDRVIDFGFGESGVDVNRLSASLGYGITDRLELFVSVPYHDFEF
                     DNDGFGDDDTSVFEDDGALDVSGLGNARAGVKWRLWGSQGDDSTGALGLFADLPTGDD
                     DVASGDAGFGVNLGMRRSNWVFELGYENQGDPEDTDECGLIPPGIPGQPSIRVLCASP
                     FGEQFNAGIGYASSISDRFDWITEVEGTFYGSLDDDDVQIFEDAVDFTTGGRLWLGAD
                     QRWAFNFALRTNLMELGDVDEFCPIGGLVGLTMFPRLAREVAPPPPPPPPPPAPAPPV
                     EAPAPPPPPPPPPPAPAPRPEERVTVNFTPGSARLSNIAKAKLDEVALRMKQDPDARA
                     LVVGHSDASGSQDGNQRMSEQRAQAVKNYLVTRHGIDPGRITTEGRGSAEATGNAEQD
                     RRAVVILTFG"
     gene            complement(7002..>7550)
                     /locus_tag="VEL74_25120"
     CDS             complement(7002..>7550)
                     /locus_tag="VEL74_25120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65886.1"
                     /translation="GAGVGDYSYLKTEFEALKRFDVSQRTFLVGRLRGGSFLYKEDAD
                     FNGLTPTAEVDQYTIPRSEYFRLDGRERLLGLDESRPGTEQILTTWEYYFPWFLNSSR
                     DFMRLEWQNWYWILYAGYGTTGFDREVYSDFNEYVPDVGLGFESSFKLRNKYRFFLSG
                     IMAQALKGSGGVEARISVKSYR"
BASE COUNT         1172 a         2668 c         2600 g         1110 t
ORIGIN      
        1 gtcggctacg gctgcaccac ctgcatcggc aacagcggcc cgctgccgcc gtcgatctcc
       61 aaggcgatcg aggagcgcga cctggtggcg gtgtcggtgc tatccggcaa ccgcaacttc
      121 gaggggcgga tcaactccga cgtgcgggcc aactacctgg cctcgccgcc actggtggtc
      181 gcctacgcgc tcgccgggcg gatcgacatc gactggcaga gcgagccggt cggcaccggc
      241 aaggatggcc agccggtcta tctgaaggac atctggccga gtcgccggga ggtggaggag
      301 acggtgctca aggccctcca cgcgcagatg ttccgcgagc agtaccagga cgtcttcgcc
      361 ggcgacgagc gctggaacgg cctcgacatc cccgaggggg acacctacgc ctgggacgac
      421 gcgtcgacct acgtgcggct ccccccctac ttcaccgaca tgccggagac gccgccgccg
      481 gtcaccgacg tgcgcggcgc ccgggtgctc ggcctgttcg ccgacagcat caccaccgac
      541 cacatctcgc cggccggctc gatcaaggcg gcgagcccgg cgggcaggta cctgcaggag
      601 cacggcgtgg cgccgaagga cttcaactcc tacggcgcgc ggcgcggcaa ccacgaggcg
      661 atgatgcgcg gcacgttcgc caacgtccgg ctgcgcaacc gcctggtgcc cgagatcgag
      721 gggggcttca ccgtccactt ccccgacggc gagccgctgt cgatctacga cgcggcgatg
      781 aagtaccaga aggagggggt gccgacggtg atcctcgccg gcaaggagta cggcaccggc
      841 tcctcccgcg actgggcggc caagggcccg ctcctgctgg gcgtgcgcgc cgtcatcgcc
      901 gagagctttg agcgcatcca ccgcagcaac ctggtcggga tgggggtcat ccccctggac
      961 ttccccgacg gccagaacgc cgacagcctg gggctcacgg ggaccgaggt ctacgacatc
     1021 gaggggctgg cccacggcgt cgagaccggc ttcgccggcg gccgcgaggt cacggtgcgg
     1081 gccactcccc aggacggcag cggcaaggtc atcgagttga aggcccgggt gcgcctcgac
     1141 accccgcagg aggtgcagta ctaccggcac ggcggcatcc tgcactacgt gctgcggcag
     1201 ttgctgggga agagctgagg tagggacctc ccctacctcc ccatcttctc ccggttgtac
     1261 tccttcgtcc cgccgcgcgg gacggagagg gtcttccagc tctccgacgc gaagtgcttg
     1321 gccaggaaga cgatctggcc cacgtggtag gccatgtggg tcatcgtccg gtcgatggcc
     1381 ttgaccacgc tgtggggctc ggcgcggatc agcacggtgc gcgggaggtc ctcctctttc
     1441 agctcggcca ggctggcgaa gaggcaggcc cacccctcct cccagcgggc gaggagcgcc
     1501 tgccgggtcg tcccctcccc ctgctcgaac tccgagtcgc ggtcgcggcc gggcttctcg
     1561 ccgtcggtgg tgaggaagtc ggtccagcgc gagcgcaggt tccccccgac gtgctggagg
     1621 agcaccgcca ggctgttgct ctcggggtcc aggaccgccg tcaggtcctc gtcccgcacc
     1681 tgggccaccg cccggtccgc cagctccttg agcttgcgga gctgctcgtc cacgtccttc
     1741 agatacaggt tcgccacgct ctcgctcatc ggcctctcct cctcccgcgt atcctaggtg
     1801 cggtagacta cgcccgcaat gtcgggtgaa tgggaagccg tgatcgggct ggaggtccac
     1861 gtccagctcc tcaccgagag caagatgttc tgtcgctgcc ccaaccgctt cggggcggag
     1921 ccgaacaccc tcgtctgccc ggtctgcctg gggtatcccg gcaccctgcc ggtggtcaac
     1981 cggcacgcgg tggacctggc cacgaagctc gccctggccc tgggttgcga ggtgcgcggg
     2041 acatcggtct tcgcgcgcaa gaattacttc tacccggacc tccccaaggg ctaccagatc
     2101 agccagttcg acaagccgct ggcggagaac ggcgcgctcg ccctcaccca gcacgagaag
     2161 agcgtgcgga tcgagcgcct gcacatggag gaggacgcgg gcaagctcct gcacgaggcc
     2221 cccggcggcg ccaagctccc cggcgagagc ctggtggact tcaaccgctg cggcgtgccg
     2281 ctggtcgaga tcgtctcgcg gcccgacatg tcgaccccgg ccgaggccca ggactacctc
     2341 cagaccctcc accagctcct gctctacacc ggcaccagcg acggcaacat ggaggagggg
     2401 agcctgcgct gcgacgccaa cgtctcggtg cggcggcgcg gcgagacccg cctgggcacc
     2461 aaggccgagg tcaagaacgt caactcgttc cgcaacgtcg cccgcgccat cgagcacgag
     2521 atccagcgcc agatccaggt gatcgaggcg ggcggccagg tggcccagga gacccgcacc
     2581 ttcgacgccg acgccggcgt cacgcgggcc atgcgctcca aggaggaggc gcacgactat
     2641 cgctacttcc cggagccgga cctgccgccg ctggtcctcg cccgggagcg gatcgacgcc
     2701 atccgcggca ccctccccga gctgccgtgg gaccgccgcg cccgcttcgc cgatgtctcc
     2761 ggggggtacg gcctgccggc cgaggccgcg cgcgtcctca ccgcctcgcg cgagctggcc
     2821 gactacttcg aggccgccgc ggccgccctg cccgccaacg ccaaggggcc caaggggatc
     2881 ggcaactggg tgatgaccga ggtgctccgc gtgctcaagg agcgcaagct ggaggtcggc
     2941 ccggatctgg agagcgccat gccgccggcc cgcctcgccg cgctggtcgc cctgcaggac
     3001 gaggggaaga tctcgagcag cgccgccaag gaggtcttcg ccgccatctg ggactcgggc
     3061 gaggagccgg ccgccgcggt cgagcgcctc ggcctcggcc aggtgagcga cagcgcccag
     3121 atcgagcgct ggatcgacga ggtgctggag gagaacccgg cgcaggtcgc ccagtaccgg
     3181 gccggcaagg tccaggtcgt cggcttcctg gtcgggcagg tgatgaagcg ctcgggcggg
     3241 cgcgccgagc ccaagggggt ccagcagctc ctgcgccaga cgctggagcg ggcgcccgtc
     3301 gtccccgaat agctcctgaa cagccttccc ccgtagcacc cggccttgat ccagttcttc
     3361 cacgtcagca agctgtaccc cgggggccag gcggccctcg ccgacgtcac gttcgacatc
     3421 ccccgcgggc agttcgtctt cctgaccggc gcgagcggcg ccggcaagac gacgctgctg
     3481 cgcatgatct tccgggagga ggtcccgagc gacggccaga tcgtggtcaa cggccgcaac
     3541 gtctcgtcga tcccgcagag caagatcccc tacctgcggc ggaccatcgg cgtcgtcttc
     3601 caggatttcc ggctcatccc ccggaagacg gtgttcgaga acgtcaccta cctcccgcgc
     3661 atcctgggct tcgacctcaa gaaccagaag cgcctcgcct accaggcgct gcgccgcgtc
     3721 ggcctcgccc accgcgccaa cgccttccct cccgagctct ccggcggcga gcagcagcgg
     3781 gtggcgatcg cccgcgcgct gatcaacgag cccgagatcc tgatcgccga cgagcccacg
     3841 ggcaacctcg acccggacct gtcgcgcgag atcctgcggc tgttcctgga ggtcaacctg
     3901 cgcggcacca ccgtcctcct cgccacccac gaccgcgaca tgatccagcg cgtcggccgg
     3961 cgggtgctga ccctggacca cgggcgcctc gccgccgacc agatgctcga ggggaccgat
     4021 ccgccggacc tcaccgagac ccgggaggac gagggggagc cgaggccgga ggccggctcg
     4081 caggcagcgc gggacgagga tccggagcgg gagccgtggc gcgagcccga ggccgcggag
     4141 cccctcgctg gcaggggaga cggcgccgga gaggacgcgc cgccatgagc ttcctccagg
     4201 cgctgctcta cttcttccgc gaggcgctgg tcaacctgct gcggagctgg aaggtggggc
     4261 tcctcgcggt gttgacgatc acggtgagcc tgctggtggg cggcgccttc ctgctgatga
     4321 gccgcaacct ggcggcctcg gtggggcagt ggcagaagga gatgcgggtg gtgatctacc
     4381 tgcggccgga taccggcgcg gacgagctgc gccggctggc cgccgaggcg ggccgcccgg
     4441 cctgggtggc tgcggtcgag ccggtgaccg ccgaagcggc aagggaccgc ttcaccgaga
     4501 tcttccccac cctgtcggac ctggtggagg gttgggagga cgagccgctc cccgcctcgc
     4561 tcgagatccg actcggcgac ttcgagcccg ccacgttgga gacctggctc gccggctggc
     4621 gccggcgccc cgaggtcagc atggtggacg acgaccgcga gtggctgcgg caggtggcga
     4681 cggcggtcgc ggtggtgcgc ggcgtggggc tcgccctggg ggcgggcctc ctggcggcgg
     4741 ccatcttcac catcatgagc gtcatccggc tgaccgccta cctccaccac gaggagatct
     4801 cgatcatgcg cctggtgggg gcgaccgagt tctacatccg cggccccttc tacgccgagg
     4861 ggctgctcca ggggctgctc ggaggggggc tcgccagcgc cggcctctac ggcgcctacc
     4921 aactgctcca cgcccgcagc cccggctccc tcttcacgac ggtcctcgcc ggcaccttcc
     4981 tgagctggag agagatcgcc ttcctcatcc tcctcggcgg cggcgccggt ctgctgggcg
     5041 ccgtggcgtc gctgcgccga gaaagcctgt agatccggga ccccttctcc gggctgccgg
     5101 aagcttccga cgcggcggcg aagctgccaa tggaccggcg gagccttcgg cgagtcgccg
     5161 aaggttccgg cgcgccgacg gaagcttgca attcgctggc gaagcttgca acgcgccggc
     5221 ggagtcttcg gcgggccgcc ggaagctcca acgcgccggc gaagctgcca acggaccggc
     5281 ggagtctccg gcgggctgtc gaaggttccg gcgcgccggc ggagcttgcg acgcgctggc
     5341 gaagcttgca acgcgctggc ggagtctccg gcgcgccggc ggagcttgcg atccgccgcc
     5401 gaagcttccg gcgcgccgtc aggaccaaag aaaaggcccc gccgggtggc ggggcctcgg
     5461 ggtcccttcg agtacgccgg ggggcttacc cgaaggtcag gatgacgacg gcccggcgat
     5521 cctgctccgc gttgccggtg gcctcggccg agccgcggcc ctcggtggtg atccggcccg
     5581 gatcgatgcc gtgccgcgtg accaggtagt tcttcaccgc ctgggcgcgc tgctcgctca
     5641 tccgctggtt gccgtcctgc gacccgctgg cgtcggagtg accgaccacc agggcgcggg
     5701 cgtcaggatc ctgcttcatc cggagggcca cctcgtccag cttcgccttg gcgatgttgg
     5761 agagcctggc gctgcccggc gtgaagttga ccgtgacccg ctcctcggga cgcggcgccg
     5821 gagccggcgg cggcggcggc ggaggcggcg gcggagccgg agcctcgacc ggcggagccg
     5881 gagccggcgg ggggggcggc ggcggcggcg gcggagccac ctcgcgggcc agacggggga
     5941 acatcgtcag gccgaccaga ccgccgatcg ggcagaactc gtcgacgtcg ccgagctcca
     6001 tgaggttggt gcgcagggcg aagttgaaag cccagcgctg gtcggcaccc agccacaggc
     6061 ggccgccggt ggtgaagtcg accgcgtcct cgaagatctg gacgtcgtcg tcgtcgaggc
     6121 tgccgtagaa ggtgccctcc acctcggtga tccagtcgaa gcgatccgag atcgagctgg
     6181 cgtagccgat gccggcgttg aactgctcgc cgaacggcga cgcgcagagc acccggatcg
     6241 acggctggcc ggggatgccc ggcgggatca ggccgcactc gtcggtgtcc tcggggtcgc
     6301 cctggttctc gtagccgagc tcgaagaccc agttcgaccg ccgcatgccc aggttgacgc
     6361 cgaagcccgc gtcacccgac gcgacgtcgt cgtcacccgt tggaagatct gcaaagaggc
     6421 cgagggcacc cgtggaatcg tcgccctggc tcccccagag gcgccacttg acgccggccc
     6481 gggcgttgcc cagaccgctg acgtcgagag cgccgtcatc ctcgaagacg ctggtgtcgt
     6541 cgtccccgaa cccgtcgttg tcgaactcga aatcatggta cggaacggag acgaacagct
     6601 ccaaccggtc cgtgatgccg tatcccaggc tcgcgctgag ccggttgacg tccaccccgg
     6661 actccccgaa cccgaaatcg atgacgcggt cccagttgtt gtaatacagg ctgaaggacc
     6721 actccccgtc tggcaaagtg tcgccactgg acaacgtaaa cagccccgtc tctcccgtca
     6781 cagtgggagc gttcgcttcc tctccggcga accccatgct ggggacgagg ctcagcagca
     6841 tgatgaaaag aaggtatttc aacttcatgc tttctcctcc ggctctgagc caataacgtc
     6901 agggctccgt gcagccctgc atcggtctta ctgcgaagac tatcgtggcg cgtgttcctc
     6961 cccggtggtc aagagtgcac gggccgctgt cggacatccg ctcagcggta ggacttgacc
     7021 gaaatacgcg cctccacacc accactccct ttcaaagcct gtgccatgat cccggagagg
     7081 aagaacctgt atttgttgcg aagcttgaaa gaggactcga aacccagccc cacatcgggg
     7141 acatactcgt tgaaatcact gtatacctcg cggtcgaagc cggtcgtgcc gtagccggcg
     7201 tacaggatcc agtaccagtt ctgccactcc agccgcatga agtcgcggct ggaattcaag
     7261 aaccatggaa aataatactc ccaggtggtc aggatctgct cggtgccggg ccgcgactcg
     7321 tcgagcccca gcaggcgctc gcggccgtcg agccggaagt actcggagcg cgggatcgtg
     7381 tactggtcca cctcggcggt cggcgtcagg ccgttgaagt ccgcgtcttc cttatagagg
     7441 aaggagccgc cccgcaggcg gccgacgagg aaggtgcgct ggctgacgtc gaaccgcttg
     7501 agcgcctcga actcggtctt cagatagctg tagtcgccga cgcccgcgcc
//
LOCUS       DASPJI010000889         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_170398, whole genome shotgun sequence.
ACCESSION   DASPJI010000889 DASPJI010000000
VERSION     DASPJI010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170398"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2859)
                     /locus_tag="VEL74_25125"
     CDS             complement(<1..2859)
                     /locus_tag="VEL74_25125"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65887.1"
                     /translation="MGPEVPVALFLERSVEAVVALVGVLAAGGAYVPLDPTYPSEWVS
                     WVLKDSRAPVVVTTSSLAGRLPADTPTLLLEEVAESPTTFDRTAGPDHPAYLIYTSGS
                     TGRPKGVVVRHGSLMNLTRALRETVYRDEAGPLRVAVNASFAFDGSVKQIVQLAWGHC
                     LHILPQDVRLDPDAMVETVRRQRLDVLDCTPSQLRPLLQAGLGERDAAPAMVLVGGEA
                     VDAELRDLALTRTRTRFWNLYGPTECTVDTVAAPFAVGTPPSCIGLPLSNVRVYVADS
                     PEDLAPLGVPGELQVGGAGLARGYLGRPDLTAERFIPDSFGGGAGERLYRTGDLVRWL
                     PNGSLDFLGRLDDQVKIRGFRIELGEIEAVLAALPEVREALVVTREDTPGLRRLVAYV
                     VGEAATETLRQALRERLPEHMVPAAFVTLPQLPLTPNGKVDRKALPPPEPERAEESWL
                     APRTPVEEVLAAIWAELLGLERVGAADSFFDLGGHSLLATRVMSRLRSAFGVEMPLRD
                     LFEAPLLADLAARVEVALRAGTGHLTPSLLPLSPEPRQGPLPLSFAQQRLWFIDQLEP
                     GSPLYNMPVTLRVEGPLDPAALASCLGEIVRRHEALRTVFTTLDDSPVQTVRPAALFV
                     LPVVDLSGLPEGEREARALALAAEEAALPFDLTRGPLLRGVLLRLAGDDHIAALTMHH
                     IASDGWSMGILVRETSALYAAFSEGRPSPLPELPLQYADFSAWQRSWLHGAILESEIT
                     FWRRQLAGLPPRLELPTDRPRPAMQSFRGALRPVRLPADLTRQVQVVSRREGATLFMG
                     LLAALQSLLARYSGQQDLAVGSPVAGRNRLEIEGLIGFFVNTLVLRGDLTGEPSFHTL
                     LGRVRETALAAHMHQDVPFEKLVEELAPERSLAQTPLFQVLFVLQNVPVQSLEARDLR
                     LRPVRGTGITSKFDLTLNLREFDGELIGMVEHATDLFDT"
BASE COUNT          472 a          974 c         1030 g          384 t
ORIGIN      
        1 ggtgtcgaac aggtcggtgg catgctcgac cataccgata agttcgccgt cgaattcccg
       61 gagattgagc gtgaggtcga acttcgaggt gatcccggtg cctctcaccg gccgcaggcg
      121 caggtcgcgt gcctcgaggc tctggacggg gacgttctgc aacacgaaca gcacctggaa
      181 cagtggggtc tgcgccaggc tcctctccgg cgcgagctcc tccaccagtt tttcgaacgg
      241 cacgtcctgg tgcatgtggg cggccagcgc cgtctcccgc acccggccca gcagcgtgtg
      301 gaacgacggc tctcccgtta gatcgccgcg cagcaccaga gtgttgacga agaacccgat
      361 cagtccttcg atctccagcc ggttgcgtcc ggcgaccggc gagcccactg cgaggtcctg
      421 ctggccgctg tagcgcgcca ggagagattg gagggcggcg aggagtccca tgaagagagt
      481 cgccccctca cgccggctca ctacctgcac ctgccgggtg aggtcggcgg gcaggcggac
      541 gggccgtagc gccccgcgga agctctgcat cgcggggcgg ggacggtcgg tggggagctc
      601 cagccttggc ggcaggccgg cgagttggcg ccgccagaag gtgatttcac tctccagaat
      661 cgcaccgtgc agccaggagc gctgccacgc cgagaaatcg gcgtactgca gcggcagctc
      721 gggcaggggc gacggccgtc cctcggagaa cgcggcgtag agagccgatg tctcgcgcac
      781 caggattccc atggaccagc cgtcgctggc gatgtgatgc atggtgagcg ccgcgatgtg
      841 gtcgtccccg gccagccgca gcagcactcc gcgcagcagc gggccgcgcg tgagatcgaa
      901 ggggagggca gcctcttcgg cggccaaagc cagggctcgt gcctcgcgct ctccctccgg
      961 cagccctgac aggtccacca cgggcagcac gaagagggcc gccggccgga ccgtctgcac
     1021 cggcgagtcg tccagcgtgg tgaagacggt gcgcaacgcc tcgtgccggc gcacgatctc
     1081 ccccaggcag gacgccagcg ccgccgggtc cagaggacct tcgacgcgca acgtcaccgg
     1141 catgttgtag agcgggctgc ccggctcgag ctggtcgatg aaccagagac gctgctgcgc
     1201 gaacgacagg ggcagcggcc cttgtcttgg ctcgggagag agggggagca gggacggagt
     1261 gagatggccc gtgccggccc gcagcgccac ctcgacccgg gccgccagat cggccagcag
     1321 cggagcctca aaaagatcgc gcagcggcat ctcgacaccg aaggcgctgc gcaggcgcga
     1381 catcacccgg gtcgccagca gcgagtgacc gcccagatcg aagaacgagt cggcggcccc
     1441 gacccgttcg agccccagga gctccgccca gatggcggcg agcacctctt cgaccggtgt
     1501 gcgcggcgcc agccagctct cctcggcccg ttcgggctcc gggggcggca gggccttgcg
     1561 gtcgaccttg ccgttggggg tgagggggag ctgcgggaga gtgacgaagg cggccggcac
     1621 catgtgctcc ggcagccgct cgcgcagggc ttggcgcagc gtttcggtcg cggcctcacc
     1681 gaccacatag gccaccagcc gccggaggcc gggggtgtcc tcccgcgtca ccaccagagc
     1741 ctcgcgcacc tcgggcagcg ccgccagcac cgcttcgatc tctcccagct cgatgcggaa
     1801 gccgcggatc ttgacctgat cgtccaggcg gccgaggaag tccagagagc cgttgggcag
     1861 ccagcgaacg aggtcgcccg tgcggtagag acgctcgccg gcaccgccgc cgaaggagtc
     1921 ggggatgaac cgttcggccg tcagatcggg ccgccccaga tagccgcggg ccagccccgc
     1981 cccgcccacc tggagctcgc caggcacacc caggggggcg aggtcctccg ggctgtcggc
     2041 gacgtagacc cgaacgttcg aaagcggcag gccgatgcag gacggcggcg tacccaccgc
     2101 gaagggcgcg gccacggtgt ctaccgtgca ctcggtaggc ccatacagat tccagaagcg
     2161 ggtccgtgtc cgagtgaggg ccaagtcgcg cagctcggcg tccacggctt caccgccgac
     2221 caggaccatg gctggcgcgg cgtctctctc tcccaggccg gcctggagga gggggcggag
     2281 ctgcgagggc gtgcagtcga gcacgtcgag ccgctgccgg cgtacggtct ccaccatcgc
     2341 atcgggatcg aggcgcacgt cctggggcag gatgtggagg caatggcccc aggcgagttg
     2401 cacgatctgc ttgaccgagc cgtcgaaggc gaacgaggcg ttgaccgcca ctcgcaacgg
     2461 gcctgcctcg tctcgataga ccgtctcgcg caatgcccgc gtcagattca tcagcgagcc
     2521 atggcggacc acgaccccct tgggccggcc cgtcgagccg gaggtataga tcaggtaggc
     2581 cggatggtcc ggacccgcgg tgcggtcaaa ggtcgtgggg ctctccgcca cctcttcgag
     2641 gagcaaggtg ggcgtgtccg ccggcaagcg tccggcgagg ctggaggtgg tgacgaccac
     2701 gggggcccgg gagtccttca gcacccagct cacccattct gacgggtagg tggggtcgag
     2761 aggcacatag gcgcctcctg ccgcgagcac gcccacgaga gccacgacgg cctcgaccga
     2821 gcgttcgagg aagagagcca ccgggacctc gggcccgatg
//
LOCUS       DASPJI010000890         2448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_170686, whole genome shotgun sequence.
ACCESSION   DASPJI010000890 DASPJI010000000
VERSION     DASPJI010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170686"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VEL74_25130"
     CDS             complement(<1..626)
                     /locus_tag="VEL74_25130"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HYG65888.1"
                     /translation="MRRNILLSIVAVMLVLAGAAQAQPFGAWVTLSGPTHGYIQIPSH
                     PSLNPTNAFTFEAWVAISNSAASEDCRSIAGKGYVQTWWLGQCTVLGQPTLRSYLKGG
                     SSARNGGVIPRGVWTHIAVTYDGAKRRHYINGELAAEFNETGSLPTNGEPMRIGSDVN
                     WTNTPAGAIDEVRLWNVARSIAQIRANLNERITTAQPGLVAVWTLDGSG"
     gene            803..1372
                     /locus_tag="VEL74_25135"
     CDS             803..1372
                     /locus_tag="VEL74_25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYG65889.1"
                     /translation="MAPGPTFRVGPGVMRIAIAGAHRTGKTTLIEELSRALPDHGVVD
                     EPYYLLEEEGYEFAEMPSLEDFEVQLERSIECVGGSEEDQIFDRCPADMLAYLIAHPD
                     SDGDVKAWLSRVRNAMQRLDLVVFVPVEDPDRIIVSDPEESDLRQRVDEELREIVVED
                     RWDFGVEAIEVAGTPRERARQVLAYLGAR"
     gene            1529..>2448
                     /locus_tag="VEL74_25140"
     CDS             1529..>2448
                     /locus_tag="VEL74_25140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65890.1"
                     /translation="MFRHLTLAFLVLAAVPAAAIAPGADPCAGSSLPYALNIGLPRLP
                     DTPGFRAVDLEQGVQKLIGTFYARHGRCPERKLQVNITVAGDYEILDWLGQGLIDVAI
                     VPDLTIHLLAGDGIRLREMDLTGHRLGGLLLPALKPQPRSARLVRFQWQRRPDPKADL
                     AAFQEQTWRLATNPPEKPEPQEYRVVFASHLSTLGFLAPLEEAGRDLERRLGSIPERE
                     KTGVRERFWQAFFDNARFAVDCDSLTRDSPGTRSCWRLPDAEERAGTGPVEILFPGEG
                     VLRPLAGPSAQVGSVPLGRYREHLVMAKRS"
BASE COUNT          389 a          790 c          854 g          415 t
ORIGIN      
        1 ccgctgccgt cgagcgtcca gacggcgacc aggccgggct gggccgtggt gatgcgctcg
       61 ttgaggttgg cccggatctg cgcgatgctg cgggcgacgt tccacagccg cacctcgtcg
      121 atggcgccgg ccggcgtgtt cgtccagttg acgtcgctgc cgatccgcat cggctcgcca
      181 ttggtgggca gggagcccgt ctcgttgaac tcggcggcca gctcgccatt gatgtagtgg
      241 cgccgcttgg cgccgtcata ggttacggcg atgtgggtcc agacgccgcg cgggatgacg
      301 ccgccgttgc gggccgacga tccgcccttg agatacgatc ggagggtggg ctggccgagg
      361 acggtgcact ggccgagcca ccaggtctgt acatagccct tgcccgcgat gctgcggcag
      421 tcctcgctgg cggcgctgtt ggagatggcg acccaggcct cgaaggtgaa ggcgttggtg
      481 gggttgagcg aggggtgact ggggatctgg atgtacccgt gggtgggacc cgacagcgtc
      541 acccaggccc cgaacggctg cgcctgggca gctcccgcga gaaccagcat gactgctacg
      601 atcgacagca agatgtttcg tcgcatctga gcctccgttt accgaatgtt gaacctatag
      661 ccaactatcg aagtgtatgc cggggcacgg ccaacagagc ggccctggta cccaccttga
      721 caggcggcgg agggggcagg ggcgttacac tccccgtgga ggaaaccaca tgcaccgatc
      781 tggagggacg gctttaccgg cgatggcgcc aggcccgacc ttccgggttg gccctggcgt
      841 gatgagaatc gccattgcag gagcgcaccg gaccgggaag acgacgctga tcgaagagct
      901 gagccgagct ctaccggatc acggcgtggt ggatgagccc tactatctcc tggaggagga
      961 gggttacgaa ttcgcggaga tgccgtccct cgaggatttc gaggtccagc tcgagcgttc
     1021 gatcgaatgc gtgggcggca gcgaggaaga tcagatcttc gatcgatgcc cggcggacat
     1081 gctcgcttat ctgatcgcac atccggactc ggacggcgac gtcaaggctt ggctttctcg
     1141 cgtgcggaac gcgatgcaac ggctcgacct ggtggtcttc gtgccggtcg aggatcccga
     1201 ccgcatcatc gtctccgatc cagaggagag cgatctgagg caacgcgtcg acgaggagct
     1261 ccgggagatc gtggtcgaag atcgatggga tttcggcgtg gaagcgatcg aggtggcagg
     1321 cacgccgcgc gagcgcgccc gccaggtatt ggcgtatctc ggagcgcgat gatccaggcg
     1381 cagtgcggcc gcccccgcta ttcttgatgc aaggctgaaa cgaaacgccg gcctcgtcgc
     1441 ctgttggaga ggagagaggt gtcccgagcg gcttcccggc ggaggaatcg ccgtccgcgg
     1501 tccgtccaac tctctcggga gacgcagcat gttccggcat ctgaccctgg ccttcctcgt
     1561 actcgccgcg gtccccgcgg ccgccatcgc tcccggcgcg gacccgtgcg ccggctccag
     1621 tctcccctat gccctgaaca tcggcctgcc gcgcctgccg gacacgcccg ggttccgagc
     1681 ggtggacttg gagcagggtg tccagaaact gatcgggacc ttctacgccc gccacggccg
     1741 ctgcccggag cgcaagctcc aggtgaacat cacggtggcc ggggactacg agatcctgga
     1801 ctggctgggg caggggctga tcgacgtcgc catcgtgccg gacctcacca tccacctttt
     1861 ggccggagac ggcatccggc tccgggagat ggacttgacc ggtcaccgcc tgggcggcct
     1921 cctgctgccg gccctgaagc cgcagccgcg ctccgcccgt ctcgtgcggt tccagtggca
     1981 acggcggccc gatcccaagg cggacctcgc ggccttccag gagcagacct ggcggctggc
     2041 cacgaatcct cccgagaagc ccgagcccca ggaataccgg gtggtcttcg cctcccatct
     2101 ctcgactctc ggcttcctcg cgcctctgga ggaggcaggc cgggacctcg agaggcgcct
     2161 cggatcgatc ccagagaggg agaaaacggg ggtgcgcgag cgcttctggc aggccttctt
     2221 tgacaacgcc cgttttgccg tcgactgcga ctccctgacg cgggactcgc cgggcactcg
     2281 gagctgctgg cgccttccgg acgccgaaga gcgggccggc accggcccgg tggagatcct
     2341 gttccccggt gaaggtgtgc tgaggccgct cgccgggccg tcggctcagg tcggctccgt
     2401 gcctctcggc cgctaccggg agcacctggt catggccaag agatcgga
//
LOCUS       DASPJI010000891         5332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_170690, whole genome shotgun sequence.
ACCESSION   DASPJI010000891 DASPJI010000000
VERSION     DASPJI010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170690"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VEL74_25145"
     CDS             complement(<1..908)
                     /locus_tag="VEL74_25145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65891.1"
                     /translation="MREIVIDHPTGEASGLLAIRNRSVLEAHGWDLGFWTCSDEDPVE
                     KALGALGRSADSPDDWELCRLPKARPSLSKKTEDCEAMARWGNHIYIFGSQSGSKPGP
                     LRPRRHFVARFDESRIQGRLDEARVDLEISRGTFRLHRLINDALRESGLELIERGPHE
                     VDRTIRKTRKKGKKLGKKWAARLHEEDWPINIEGVAFRTNGALLVGLRYPVTRDGHPI
                     LVEVDGIDRYFFDPPESEPAVTGLWVLADVGSAAEPRGVRAIEEDVEGGCFHVITGSL
                     ESDPEQSVLLRDHPEGAEAASRHHRVT"
     gene            986..4084
                     /locus_tag="VEL74_25150"
     CDS             986..4084
                     /locus_tag="VEL74_25150"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYG65892.1"
                     /translation="MTTSAPRPFAPVPWFSRRPFVCLALLAAGLSGACAAPAPPSPRL
                     EKGVPVERELRGGQRHEYRMELPAGWYARLHIPQPGVDVAAVLYAPEGSMVAGTAGPS
                     GIDEEEVVSLVTSRQGEYRLVVTPRDPQGPPGRYLLVLRELRPAAATDRRRVAAERAM
                     EEGRRYARVIATVPLAIERYGVALGLWQAIGDRRGEVDSLNLLGPAYAADGAKAVDTC
                     RQAIQRARQAGYHEGEARALKNCGDALSNEGRLQEARAMFERSLALWQAIGDRSKQHV
                     LLQNIGQIYLTLRQPQRALDIYRQSLALATAPAERANALAGTGACHWALRDFDAALTH
                     LLRARALIHETGDGENEGRILGLIADIYQRRGQLQEALRACRQGLGVAKAQDARAYLL
                     YLQANLLIDLGDLEQALANYQEALRLYHRIGERHWQNLCRAGIGWVRLHQGEYRKALA
                     EYEQIAGGGFSYPRALVYWALDQPGRAAGLLEKALAQARAEGDLAKEGEIQLALGTTY
                     RDLGRSREAARSLTRSVELGRRLDYPGVLTASLFRLAQLERDGGRLGRARDLAEEALG
                     IVESLRSRVAGRELGTSFFASRRSYYELYVDLLLRLDQLHPGQGYLEAALSASERARA
                     RVLLDLLAESRFAREGRRPGEPRQSPQRSDLRAGASASLGLDEIRALLDEDTALLEYT
                     LSAERSNLFVVTRERLEVFPLIGAEEIDRKVHRLRQLISKPQGRRIRAYAAVAHDLYQ
                     DLVAPAADILAHKRRLVIAPDRSLYLLPFEVLLTAPATGDRLGEMPFLIQRYAISNVP
                     SASVLARLREPAPGVAPRPAAARRRFVAFADPPPQSSGLFPRLPPLPEARREVTQIAR
                     LYGTGGARLYEGTEATEDNVKDNDQVATAGYLHFATHSLLDEKQPERSGLVLARRPGS
                     REDGFLQAFEVFDLDLSADLVVLSACDSGGKEVTGEGLVGLTRAFLYAGAPTVAVSLW
                     KVDDASSADLMVHFYHQLARTSDKAEALRRSKRLMIADPALAHPFYWSSFVLVGDPGR
                     RP"
     gene            4396..4695
                     /locus_tag="VEL74_25155"
     CDS             4396..4695
                     /locus_tag="VEL74_25155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65893.1"
                     /translation="MSILDDEEKSESRSGDGVPKVEATPPPPPPVNHVLSILFAVLAL
                     GAVFLRAWGPGVAFAATSLAFNVVAFVQNRAWSKRVADAWPTGTPPRPAGRGPGG"
     gene            4766..>5332
                     /locus_tag="VEL74_25160"
     CDS             4766..>5332
                     /locus_tag="VEL74_25160"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HYG65894.1"
                     /translation="MNLSRILLSGALALVLAPVFLASLHSAQAAPEDKAAAPVIVELF
                     TSQGCSSCPPADRLLTRLGGDPGLAGRVIPLAFHVDYWNHIGWTDPFSAKRWSERQQA
                     YARAFKANRIYTPQLVVNGRTEGVGSHEPRVRKQIADALAREPAGRVSLGVETPAAGQ
                     VRVKVGARLARQARQETAGDLDAWVAVVE"
BASE COUNT          764 a         1864 c         1875 g          829 t
ORIGIN      
        1 gtgacgcggt ggtggcgcga ggcggcctcc gccccttccg gatggtcccg cagcaggacg
       61 ctctgctccg gatcgctctc caggctgccg gtgatgacgt ggaagcagcc cccctcgacg
      121 tcctcctcga tcgcgcgcac cccccggggc tcggcggcgc tgccgacgtc ggcgagcacc
      181 cagaggccgg tcaccgcggg ctcgctctcg ggcggatcga agaagtagcg gtcgatgccg
      241 tcgacctcca ccaggatcgg gtggccgtcc cgggtcaccg ggtagcgcag gccgacgagg
      301 agcgcgccgt tggtgcggaa agccacccct tctatattga tgggccagtc ctcctcgtgg
      361 aggcgcgccg cccacttctt gcccagcttc ttccccttct tgcgggtctt gcggatggtg
      421 cggtccacct cgtggggccc gcgctcgatc agctcgaggc cgctctcgcg cagggcgtcg
      481 ttgatcagcc ggtgcagccg gaaggtgccg cgggagatct cgaggtccac ccgcgcctcg
      541 tcgagccgcc cctggatgcg gctctcgtcg aagcgggcga cgaagtggcg gcgcggccgc
      601 agggggccgg gcttggagcc ggattgggag ccgaagatat agatatggtt gccccagcgg
      661 gccatcgcct cgcagtcctc ggtcttcttc gagagcgacg gccgcgcctt gggcagccgg
      721 cacagctccc agtcgtccgg cgagtcggcg gaccggccga gcgcccccag cgccttctcg
      781 accggatcct cgtcggagca ggtccagaag cccaggtccc acccgtgggc ctccagcacc
      841 gagcggttgc ggatggcgag gagcccggaa gcttcgccgg tcgggtggtc gatcacgatt
      901 tcacgcacga gaaaggcttc tagcaacgcc catgccgcac ctattggagt aagatggagt
      961 ttccctctgg aggtcgagag tcctcgtgac gacctcagcg ccccggccct tcgcgcccgt
     1021 tccctggttc agccggcgtc cgtttgtctg cctggccctg ctggcggccg ggctgtctgg
     1081 ggcctgcgcc gcgccggcgc ccccttcacc ccggctggag aagggggtgc ctgtggagcg
     1141 ggagctgcgc ggtgggcaga ggcacgagta ccggatggag ctgccggcgg gctggtacgc
     1201 ccggctccac atcccccagc ccggcgtcga tgtggccgcg gtcctctacg ccccggaggg
     1261 aagtatggtt gccggcactg cggggccttc cgggatcgac gaagaggagg tcgtctccct
     1321 ggtgacgagc cggcaggggg agtaccggct ggtcgtcacg ccgcgtgacc cgcaggggcc
     1381 gccggggcgc tacctgttgg tgctccggga gctgcggccc gccgcggcca ccgaccggcg
     1441 gcgcgtcgcc gccgagagag ccatggaaga ggggcgccgg tacgcacggg tgatcgcgac
     1501 agtgccgctg gctatagaaa ggtatggcgt ggcactcggc ctgtggcagg ccatcggtga
     1561 tcgccggggc gaggtggaca gcctgaacct cctgggtccg gcctacgccg ctgatggcgc
     1621 caaggcggtc gacacctgcc ggcaggccat ccaacgagcc cggcaggcgg gctaccacga
     1681 gggcgaggcg cgggctctga aaaactgcgg ggatgctctc tcgaatgagg ggcggttgca
     1741 ggaggcgcgc gcgatgtttg agcgctcgct cgcgctctgg caggcgatcg gcgaccggag
     1801 caagcaacac gtgctgctcc agaacatcgg tcagatatac ctcaccctca ggcagccgca
     1861 acgggcgctc gacatctatc ggcagtcgct ggctctcgcg acagccccgg ccgaacgcgc
     1921 caatgcgctg gcgggtacag gcgcctgtca ctgggcgctt cgtgacttcg atgccgcgct
     1981 cactcacctg ttgcgcgccc gcgccttgat ccacgagacc ggagacgggg agaacgaagg
     2041 gcgcatactg ggtctgatcg cggacatcta tcagcggcgc ggccagctcc aggaggcgct
     2101 ccgcgcctgc cgtcaaggcc tcggcgtggc gaaggcgcag gacgcgcgcg cctatctcct
     2161 ctacctccag gcgaacctgc tcatcgacct gggggacctg gaacaggccc tcgccaacta
     2221 ccaggaggcc ctccgcctct atcatcggat cggcgagcgc cactggcaga acctctgccg
     2281 tgccgggatc ggctgggtcc ggttgcatca aggcgaatac cggaaagcgc tggcggagta
     2341 cgagcagatc gccggcgggg gattctccta cccgcgggct ctggtctatt gggccctcga
     2401 ccagccgggc cgggccgccg gtctgctcga gaaggctctg gcgcaagccc gggcggaagg
     2461 agacctcgcg aaggaggggg agatccaact cgctctcggc accacgtacc gggacttggg
     2521 gcgctccagg gaggcggccc ggtccttgac ccggtcggtg gagctggggc ggcggctcga
     2581 ctaccccggt gtgctgaccg cgagcctctt ccggctcgcc cagctggagc gcgacggggg
     2641 gaggctgggg cgcgcccggg atctcgccga ggaggccctg gggatcgtcg agtcgctgcg
     2701 cagccgagtc gccggacgcg agctggggac ctccttcttc gcctcccgcc gctcgtacta
     2761 cgagctctac gtcgacctgc tgctacgact ggaccagctc cacccggggc agggctacct
     2821 cgaggcggcc ctgtccgcca gcgagcgggc ccgtgcccgc gtcctcctgg acctcctggc
     2881 cgagtcccgg tttgccaggg aaggccggag gccaggggag ccccggcaga gcccgcagag
     2941 gtccgatctg cgcgccgggg cctccgcctc gctcggcctc gacgagatcc gtgccctcct
     3001 ggacgaggat acggcgctgc tcgagtacac actgagtgcc gagcgctcca acctgttcgt
     3061 cgttacccgc gagcgcctgg aggtctttcc tctcatcggg gcggaggaga tcgaccgcaa
     3121 ggtgcaccgc ctgcgtcagc tcatctcgaa gccgcagggg cgccggatac gcgcttatgc
     3181 cgcggtcgcg cacgatctct atcaggacct ggtcgccccc gccgccgaca tcctcgccca
     3241 taagcgccgt ctggtcatcg cgccggaccg ctcgctctat ctgctgccgt tcgaggtgct
     3301 gctgaccgcg cccgcgaccg gcgaccggct gggagagatg ccgttcttga tccagcggta
     3361 cgcgatcagt aacgtcccgt cggccagcgt gctcgcccgg ctgcgcgagc ccgcgccggg
     3421 tgtggcgccc cggccagccg cggcgcgtcg gcgtttcgtc gccttcgccg acccgccgcc
     3481 gcagagcagc gggctctttc cgcggttgcc cccgctgccg gaggcacggc gtgaggtcac
     3541 ccagatcgcc cgcctctatg gcacgggagg agcgcggctc tacgagggaa ccgaggccac
     3601 agaggacaac gtcaaggata atgaccaagt ggcgacagcc ggctacctgc attttgcgac
     3661 tcacagcctg ctcgacgaga agcagcccga gcgctccggt ctggtcctgg cccgcaggcc
     3721 tggtagccgc gaggacggtt tcctgcaggc cttcgaggtc ttcgaccttg acctctcggc
     3781 cgacttggtg gtgctctccg cctgtgactc cggcggcaag gaggtgacgg gcgaggggct
     3841 tgtcggcctc acccgcgcgt ttctctacgc gggagccccc acggttgcgg tgagtctctg
     3901 gaaggtggat gatgcctcgt cggccgacct catggtgcac ttctaccacc agctcgcgcg
     3961 cacgagcgac aaggccgagg ccctgcgccg ctccaagcgc ctgatgatcg ccgatcctgc
     4021 tctggcccac cccttctact ggtcgtcctt cgtcctggtg ggagacccgg gccggcgtcc
     4081 gtaacattcg aggccctgag gcgcctgtga aagcgaagcc cagcgatccg gagagatgat
     4141 tcacaacaag ggaggactca tcatggcgac cctggacaag actacggaat ccagcagcgg
     4201 agacggcaca cccaaggtca gggcgacccc gccgccgccg ccgccttctt cctgagcttc
     4261 gtctgcggac gggacagggg cgagccgcct gcgagcggtt ggaggggaat ccttgcatgg
     4321 cgattcgatg cgaacaggct cctccgccgt ggtgggtggg ggggcgatca tggtcttgga
     4381 ccgggaggat cccagatgtc cattctggac gatgaagaaa agagcgaatc gcgcagcgga
     4441 gacggcgtgc ccaaggtcga ggccacccct cccccccctc ctccagtgaa tcatgtgctg
     4501 agcatcctct tcgcggtgct ggcgctgggg gccgtgttcc tccgcgcgtg gggtcccggt
     4561 gtggccttcg cggcgacgag cctggcgttc aacgtcgtgg cttttgtcca gaatcgcgcc
     4621 tggtcgaagc gcgtggccga cgcttggccg accgggactc ctcccaggcc tgccggcagg
     4681 ggaccgggcg gctgagaacc acccggcccc tctcatccgt acactccttc cgggggcgct
     4741 ctccgctccc cggaaggagc ccatcatgaa tctctcccgc atcctgctct ccggcgcgct
     4801 ggccctcgtc ctggcgccgg tgttcctcgc ctccctccac tcggcccagg cggccccgga
     4861 ggacaaggcg gctgctccgg tgatcgtcga gctgttcacc tcccagggct gctcgagctg
     4921 cccgccagcg gaccggctgc tcaccaggct cggcggcgac cccggcctcg ccgggcgggt
     4981 gatccccctg gccttccacg tcgactactg gaaccacatc ggctggaccg acccgttctc
     5041 ggccaagcgc tggtcggagc gccagcaggc ctacgcccgg gcattcaagg cgaaccggat
     5101 ctacacgccc cagctcgtgg tcaacggccg caccgagggc gtcggctccc acgagccgag
     5161 ggtccgcaag cagatcgccg acgccctggc cagggagccc gccggccggg tgagcctcgg
     5221 ggtggagacg cccgcggccg gccaggtgcg ggtgaaggtc ggggcgcggc tggcgcggca
     5281 ggcacggcag gagacagcgg gggacctgga cgcctgggtc gcggtggtcg ag
//
LOCUS       DASPJI010000892         5909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_282732, whole genome shotgun sequence.
ACCESSION   DASPJI010000892 DASPJI010000000
VERSION     DASPJI010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282732"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="VEL74_25165"
     CDS             <1..1635
                     /locus_tag="VEL74_25165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HYG65895.1"
                     /translation="WLPFWAAFATAVVLRFADLGARPLHHDEGVNAWMVWKFIRGEAY
                     RYDPANFHGPFLFFYAWLPLRLFGLGDTVLRLLVAPVSALMLPLLLPLRRRLGVAGVT
                     GAAWLLAVSPAFVYYGRDLIHETFLAFFTLAMVVCGSLYAETRRERWLMLAAVCLGLL
                     PTIKETFVLTLGTLAVAGLAAWFWTSPGERDPMTPAWIRPRAVALALAVAAVVYVLFF
                     TSFFIHWQGLFDSVRGLILWTRRGAGGDGHEKPWFYFLKLLWEFETATALCALAGAGL
                     AFWRRNGFGVLCALWALGQLALYSAIPYKTPWLVVNILLPAALAAGVFFRELTGQVAE
                     REPSTRESSPPGPLSHLPPTPVPGEGETSLDLPFSSSPGGGRLGDGRGGWGVRTPRAS
                     RPAWLSPAILALVALGLVWMGWRTVETSFLRHDDDALTLVYVPTTRQTRDLIATVEEA
                     VARVPKGRRPEVRVFLPYSWPLPWYFRDLEGVGVWTEVQKRFADGDVVLVEGSQDPKL
                     RPLMKDRYVRRRYELRPRTPVVLYVNERLNPPPSAP"
     gene            complement(1610..1957)
                     /locus_tag="VEL74_25170"
     CDS             complement(1610..1957)
                     /locus_tag="VEL74_25170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65896.1"
                     /translation="MTDERPIVCVDLNGVLDSYTGWRGPEHWDAPRPGAEDFLRALND
                     RGFRVVVFTTRWGDDARGWLEAHGLHRWVAEVTDRKPAAHVFVDDRAVCFRGDFGETL
                     EQIAAFRAHWEEG"
     gene            complement(1954..3633)
                     /locus_tag="VEL74_25175"
     CDS             complement(1954..3633)
                     /locus_tag="VEL74_25175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HYG65897.1"
                     /translation="MESDTAPRPELRPLDLAGLAVVAATARTFNLGTFSLWLDEILLM
                     LRARGGPAEVWAACRANAEHPPLSALVTAAMNAAGATETVHRLLTVALGVATVLLLAA
                     WAGRRFGRAAGLVTGLVTALVPFHVRYSQELRPYVYLLLFAALTLLASDRLERRPGPG
                     SAALACAAILGGFYSHHLFALVLIPAAWPLVEAALRRKNRRALALFAVAAGVAFLAFV
                     PWLLAITGELSGREAAGGARSWTWRLALQRWQFLTVGALEGDGITWAGFLALALAVLG
                     AAAALRSPAGRAAVAGAVLGIGGAEIFMAATDHWSSGRYDSAGWLFLPVLIGLGIARI
                     SEIRPSRRESSPPGPLSHLPPTHTPGEGETSTDLSFSPSPGGGRWGDGRGGRGVRTPR
                     ADAAFRGTLLAALVLAALLVGQGLGLARYARAGRSDWDQVAEAVRRVHRPGEPVLVEN
                     EWTRISLGYYLQGPDFQNRQGEDGAPVLVGAGPRRLRQLWPADRRAVLVVAGIPRQQP
                     LRRWARAFPVAGRFPRSNARVFLLTPEVRQRLFDSGVRAAGPARMLLGSGS"
     gene            3724..5046
                     /locus_tag="VEL74_25180"
     CDS             3724..5046
                     /locus_tag="VEL74_25180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HYG65898.1"
                     /translation="MSAKGVHIEAPEGRLGVLLVGLGAISTTVIAGVEAIRRGLGKPI
                     GSLTQMGTIRLGKRTENRVPRIADFVPLAGLDDLVFGAWDIFPDNGYEAAVEAGVLEK
                     QLLETLRPELEAIRPMKGVFDPRYVKRLHGTHIKEGRTKADLARQLIDDIQRFQEENR
                     VDRLVMVWCGSTEGHMTPGAVHQSLEAFEAGLEANDIEIPSSMVYAYAALSMGIPFAN
                     GAPNLTVDVPALLELAEQRQAPVCGKDFKTGQTLMKTILAPGLKARMLGLSGWFSTNI
                     LGNRDGEVLEDPESFKTKEQSKLSVLEHILQPHLYPDLYEGFHHQVRINYYPPRGDNK
                     EGWDNLDIFGWLGYPMQIKIDFLCRDSILAAPLVLDLALFLDLARRAGMHGIQEWLSF
                     YFKSPMTLPELYPEHDLFIQLMKLKNTLRYLRGEEQITHLGIEYYEAD"
     gene            5612..>5909
                     /locus_tag="VEL74_25185"
     CDS             5612..>5909
                     /locus_tag="VEL74_25185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65899.1"
                     /translation="MQDNLKGTTVPEPQHLNAFSSSFLNRLAERDEPPTAGDADVAGP
                     WRIEPVPGSGFGLFRVGESLSRGFAPAAVFPSRWLALVCAAILPGTGRDPLLILS"
BASE COUNT          871 a         2128 c         2033 g          877 t
ORIGIN      
        1 tggctgccgt tctgggccgc cttcgccacg gccgtcgtcc tgcggttcgc cgacctgggc
       61 gcgcgccccc tccaccacga cgagggggtc aacgcctgga tggtctggaa gttcatccgc
      121 ggcgaggcgt accggtacga ccccgccaac ttccacggcc cgttcctttt cttctatgcc
      181 tggctccccc tgcgcctctt cggcctgggg gacacggtgc tgcggctgct ggtggcccca
      241 gtctcggcgc tcatgctgcc tctcctcctg cctctacggc ggcgcctggg cgtggccgga
      301 gtcaccgggg cggcctggct gctcgcggtc tcccccgcct tcgtctacta cggccgcgac
      361 ctgatccacg agacgttcct cgccttcttc accctggcca tggtggtctg cggctcgctc
      421 tatgccgaga cccgccggga gaggtggctg atgctcgcgg cggtctgcct cgggctcctc
      481 cccacgatca aggagacgtt cgtcctcacc ctcgggaccc tcgcggtggc cgggctggcg
      541 gcctggttct ggacgtcgcc gggggagcgg gacccgatga cccccgcctg gatccgtccc
      601 cgggccgtag ccctcgctct tgccgtagcc gccgtcgtct acgtcctctt cttcacctcg
      661 ttcttcatcc actggcaggg gctgttcgat tctgtccgcg gcctgatcct ctggacccgc
      721 cgcggggccg ggggcgacgg gcacgagaag ccctggttct acttcctgaa gctgctctgg
      781 gagttcgaga ccgcgaccgc cctctgcgcc ctggccggcg ccgggctcgc cttctggagg
      841 aggaacggct tcggcgtcct ctgcgccctc tgggccctgg gccagctcgc cctctactcg
      901 gccattccct acaagacccc ctggctggtg gtgaacatcc tcctgccggc ggcgctcgcg
      961 gcgggggtgt tcttccggga gctcaccgga caggtggctg aaagggagcc gtcgacgcgc
     1021 gagtcctcac cccccggccc cctctcccat ctcccgccca cccctgtccc gggagagggg
     1081 gagaccagcc tggatctgcc tttctcctcc tctcccggcg gggggcggtt gggagatggg
     1141 agagggggtt ggggggtgag gactccgcgg gcatcacgtc ccgcttggct cagcccggcc
     1201 atcctcgccc tggtcgccct cgggctcgtc tggatgggct ggaggaccgt cgagacctcc
     1261 ttcctccgcc acgacgacga cgcgctcacc ctcgtctacg tcccgacgac gcgccagacg
     1321 cgggacctga tcgcgacggt ggaggaggcg gtcgcccggg tgccgaaggg ccgcaggccg
     1381 gaggtgaggg tcttccttcc ctactcgtgg ccgctcccct ggtacttccg ggacctggag
     1441 ggggtggggg tctggacgga ggtccagaag cggttcgcgg acggcgacgt ggtcctcgtc
     1501 gaggggagcc aggatccgaa gctccggccc ctgatgaagg accgttacgt ccggcgccgg
     1561 tacgagctgc ggccgcggac gccggtggtg ctgtacgtca acgagcggct caaccctcct
     1621 cccagtgcgc cctgaacgcg gcgatctgct ccagggtctc gccgaagtcg ccgcggaagc
     1681 agacggcgcg gtcgtcgacg aagacgtggg cggcgggctt gcggtcggtg acctcggcga
     1741 cccagcggtg gaggccgtgc gcctccagcc acccccgggc gtcgtccccc cagcgggtgg
     1801 tgaagaccac gaccctgaac ccccggtcgt tgagcgcccg caggaagtcc tcggccccgg
     1861 ggcgcggcgc gtcccagtgc tccggccccc gccagccggt gtagctgtcg aggacgccgt
     1921 tcaggtccac gcagacgatc gggcgctcgt cggtcatgac ccgctcccga gcagcatccg
     1981 tgccggtccc gccgcccgga cgccggagtc gaagaggcgc tgccggacct cgggggtgag
     2041 caggaagacc cgcgcgttgc tgcgcgggaa gcggccggcc actgggaagg cccgcgccca
     2101 gcggcggagc ggctgctggc gcgggatgcc ggcgacgacc agcacggccc gccggtccgc
     2161 cggccagagc tggcgcaggc ggcgcggccc ggcccccacc agcacgggcg cgccatcctc
     2221 tccctggcgg ttctggaagt ccgggccttg taagtaatac ccgaggctga tgcgggtcca
     2281 ctcgttttcc accagcaccg gctcgccggg gcggtggacc cgccgcacgg cctcggccac
     2341 ctggtcccag tcggagcggc cggcccgcgc gtagcgcgcc aggcccaggc cctgccccac
     2401 gagaagggcg gcgaggacga gggcggctaa gagggtgcct cgaaaggcgg cgtctgcgcg
     2461 tggagtcctc accccccggc cccctctccc atctccccac cgccccccgc cgggagaggg
     2521 ggagaaagac aaatccgtgc tggtctcccc ctctcccggg gtatgggtgg gcgggagatg
     2581 ggagaggggg ccggggggtg aggactcgcg cctcgatggc cggatttctg agatacgggc
     2641 gatccccaag ccgatcagga ccgggaggaa cagccagccc gccgagtcgt agcgtccgct
     2701 gctccagtgg tccgtcgccg ccatgaagat ctcggcgccg ccgatcccca gcaccgcccc
     2761 ggcgaccgcg gcgcgcccgg ccggcgagcg cagggccgcg gcggctccga ggaccgccaa
     2821 agccagcgcc aggaaacccg cccaggtgat gccgtccccc tccagggctc ccacggtgag
     2881 gaactgccag cgctggaggg cgaggcgcca cgtccacgac cgggctcccc ccgcggcctc
     2941 gcggccggag agctcgccgg tgatggcgag cagccagggg acgaaggcca ggaaggcgac
     3001 cccggccgcg acggcgaaga gggcgagcgc ccgccggttc ttcctcctga gggccgcctc
     3061 tacgagcggc caggcggccg ggatcagcac cagcgcgaac aggtggtggg agtagaagcc
     3121 gccgaggatc gccgcgcacg ccagggccgc cgatccgggc cccggccgcc gctccagccg
     3181 gtcggacgcc agcagggtga gcgccgcgaa gaggagcagg tagacgtagg gccgcagctc
     3241 ctgggagtag cggacgtgga aggggacgag cgcggttacc agccccgtga caagcccggc
     3301 ggcgcggccg aaccgccggc ccgcccacgc cgccaggagc agcacggtgg cgacgccgag
     3361 cgcgacggtc agcagccggt ggaccgtctc cgtggccccc gcggcgttca tcgccgcggt
     3421 caccagggcc gacagcggcg ggtgctccgc gttggcccgg cacgcggccc acacctccgc
     3481 cggtcctccc cgggcgcgca gcatgaggag gatctcgtcc agccagagcg aaaacgtccc
     3541 gaggttgaac gtccgggcgg tcgccgccac gacagccagg ccggcgagat ccagcggccg
     3601 gagctccggg cgcggcgcgg tgtcggattc catgctcgca gcatgctaat ccaaaaccca
     3661 tggcgcgtgg gttgcatgtt cacgctgctt tgtagaagcc gtttcttcga cggaggggga
     3721 gccatgagcg ccaagggcgt gcacatcgaa gcaccggaag gcaggctggg agtgctgctg
     3781 gtgggactcg gagccatcag caccacggtg atcgctgggg tggaggcgat ccggcgcggt
     3841 ctgggcaagc ccatcgggtc gctgacccag atgggcacca tccgcctcgg caagcggacc
     3901 gagaaccgcg tgccccgcat cgccgacttc gtccccctgg ccggcctgga cgacctggtc
     3961 ttcggcgcct gggacatctt cccggacaac ggctacgagg ccgccgtcga ggccggggtc
     4021 ctcgagaagc agctcctcga gacgctgcgg ccggagctgg aggcgatccg cccgatgaag
     4081 ggggtcttcg atccccgcta cgtcaagcgc ctccacggca cccacatcaa ggaggggcgc
     4141 accaaggccg acctcgcccg ccagctcata gacgacatcc agcgcttcca ggaggagaac
     4201 cgggtggacc gcctggtcat ggtctggtgc ggcagcaccg agggccacat gaccccgggc
     4261 gcggtccatc agtcgctgga ggccttcgag gcggggctgg aggcgaacga catcgagatc
     4321 ccgtcgagca tggtctacgc gtacgcggcc ctgtccatgg ggatcccctt cgccaacggc
     4381 gcgccgaacc tgacggtcga cgtgccggcg ctgctggagc tggccgagca gcggcaggcg
     4441 ccggtctgcg gcaaggactt caagaccggc cagacgctga tgaagaccat cctggcgccg
     4501 gggctcaagg cgcgcatgct ggggctgtcc ggctggttct ccaccaacat cctgggcaac
     4561 cgggacggcg aagtgctgga ggaccccgag tccttcaaga ccaaggagca gagcaagctg
     4621 tcggtgctgg agcacatcct ccagccccac ctctaccccg acctctacga ggggttccac
     4681 caccaagtgc ggatcaacta ctacccgccg cggggggaca acaaggaggg gtgggacaac
     4741 ctcgacatct tcggttggct cggctacccg atgcagatca agatcgactt cctctgccgc
     4801 gacagcatcc tcgccgcgcc gctggtgctc gacctcgccc tcttcctgga cctcgcccgc
     4861 cgcgccggca tgcacggcat ccaggagtgg ctctccttct acttcaagag cccgatgacg
     4921 ctccccgagc tgtacccgga gcacgacctg ttcatccagc tcatgaagct caaaaacacc
     4981 ctgcgctacc tccgcggcga ggagcagatc acccacctgg ggatcgagta ctacgaggcg
     5041 gactgaaccc tcaccccccg gggagagggg gaggcggata ggggagtcta gctaaataga
     5101 tgtaggggag gggcccctcc cgggtggtgg tgcctccccc cctgggaggg tgggtggtag
     5161 ggagtggggg aggtcaggag gggggcagct tcttcttctt tttcttcttc ttcatttcgc
     5221 cgcagatccg agcggatccc ctccgccctc ccccaaagca acccccctcc tcggtcctcc
     5281 cccactccct accgcccacc ccccaggggg gaggaagccc cctcccaatc tacgaagggt
     5341 tgaatccgtg ggaaggttca gcgtccccgg gcagaaaatt gttctccgcc ctattgacac
     5401 cctctccttc ccttcgatag gcttcgaaat caggacaaag cccggcgcat agttcttccc
     5461 gggaggaagg gggatcgagg agaagaggaa gaagaggcag gaccccacga cttgagcggg
     5521 gcaggccggc gcgtggctgg cttgcggccc ggaagtgagt tggcgctcac ccccaggcct
     5581 ttcccacaca aacctcgtta gcggaggatt tatgcaggac aacctgaagg gtaccactgt
     5641 acccgaaccg cagcatctca acgctttcag cagctccttc ctcaaccgtc tggccgagcg
     5701 ggacgagcct cccacggccg gtgacgccga cgtggccggc ccctggcgga tcgagcccgt
     5761 tccgggatcg ggcttcggcc tgtttcgggt gggcgagagc ctctcccgcg gcttcgctcc
     5821 ggcggcggtc ttcccgtccc ggtggctcgc gctcgtctgc gccgccatcc tccccggcac
     5881 cggccgggac cctctcctga tcctctcca
//
LOCUS       DASPJI010000893         6497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_736836, whole genome shotgun sequence.
ACCESSION   DASPJI010000893 DASPJI010000000
VERSION     DASPJI010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6497)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6497
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736836"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VEL74_25190"
     CDS             complement(<1..485)
                     /locus_tag="VEL74_25190"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYG65900.1"
                     /translation="MPGRERGRAPRLAAPVLFVLFVGFGTGAARSALPPGYTNTLVTT
                     VASPTSVRFTPDGRMLITTQPGDLRVYQGGALLPTPALTFPAAELCTNREQGLSSVAV
                     DPDFPSNGFIYLYRTFKRPDGVCVNRASRFVLPPGNVVDLAGEVVLVDNIPSPFGTHN
                     AG"
     gene            complement(555..3011)
                     /locus_tag="VEL74_25195"
     CDS             complement(555..3011)
                     /locus_tag="VEL74_25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013771431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HYG65901.1"
                     /translation="MSKQSIRAILVTLLILAAGEVRAAVPTGFTDALVTNVGAPTALA
                     FTPDGRLLITTQGGSLRVYQGSALLATPALTIPANRLCNSSEQGLLGIAVDPAFASNG
                     FIYLFYTFERPSPPGGCVNRVSRFTLPANNVINSITELVMVDNMPSPAGNHNAGDVQF
                     GRDGYLYISIGDGGCDYANDSGCAESNDAARDEHVLTGKILRITKDGGIPPSNPFQGA
                     GTARCNVGGSTTPGNKCQETFAWGLRNPFRMAFDPNAAGTRFFINDVGQGAWEEIDLG
                     QAGADYGWNCREGAHPRNTSGPCNPAPPNMVDPIHEYSHNTGCGSITGGAFVPNGTWP
                     AAYDNGYLFSDYVCGRIFKLTPAAGGGYTATDFATGLGSSSAVHLTFGPFGNAQALYY
                     TTYAGGGQVRRISYNVPGTNNAPTAVASASPTSGSAPLTVTFNASGSSDPDTGNSLTY
                     FWSFGDGTPEVSTTSLTINHTYASAGNYTATLRARDNNLAFSSPVTVQIQSGNTPPAP
                     VISSPAAGALFRVGQSVTLSGSASDAQDGTLPASRLSWTVVLHHDTHTHPFFGPAAGN
                     NVTFTTPAPEDLAAAANSYLEVRLTATDSQGLSSTVTRDFQPRKVNVTFATAPAGLQV
                     TVNGTTLTGPQTVISWDGYRLNAVAPTQDAAGQTWVFASWSDGGAASHAIVTPASPAT
                     YTATFEASDASIPLDFHTVTPCRVVDTRDPAGPLGGPALASGSTRTFDVAGACGIPAS
                     AAALAVNVTVIAPTRSGYIQLFPAGLSASGTSTVNFAAGQTRANNAIVSLGSNGFSAT
                     CAMGGAGSAHLLVDVYGYFE"
     gene            3089..3988
                     /locus_tag="VEL74_25200"
     CDS             3089..3988
                     /locus_tag="VEL74_25200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65902.1"
                     /translation="MPRLLRLLLLLLCISLPAAAQPLRWEVEGPPSLAPVAERLRGLE
                     PDRLVGAMRLVGLEDAGPPIRVIVAPEGSEAARAVPPWVSGYALSEQGIVVILPARTP
                     TYPDSSLPDLFRHEIAHVLVARAAGGGEVPRWFHEGVAMVAGLTWGLDDVSRASLAVM
                     VRGEVPLATLDRAFGGGHGEVQRAYAIAGTFVRDLLERHGQDVAGQILAGVRQGLPFE
                     EAFARATGETLAAAEAGFWERQSFWYRWVPLLTSSVTLWLGITLLAVWAIRRRRKRDA
                     ALRARWDEEEKRLAEVSLDEPVN"
     gene            complement(4014..6065)
                     /locus_tag="VEL74_25205"
     CDS             complement(4014..6065)
                     /locus_tag="VEL74_25205"
                     /inference="COORDINATES: protein motif:HMM:NF024972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HYG65903.1"
                     /translation="MVRIGRLLQGICVFAAALAAPAALTAQPDFRIPAIRATGPGEVA
                     VAPDLQSPAEVVTLDQGVAPLLLSIPPDAEVRLGDWPIAPELRADVVLHRREIYAPDA
                     KIYEVRGKRLVEVPRSNLVFYWGAAEVDEDVRVFLSVDPATGEVRGFSQSIDGIQELR
                     PLPADGPQAQHMVASSEAFEAGDKALTGGETDGPRFTCGEEDLPQPLTKASAPSSLGS
                     VSEKVLSSIHTAVIAVDTDNELMSLKFSNNTASATSYIANLIAKMNVFYERDLKIRLL
                     QGHTILRPSTTADPYAQSGTGSADSAKLNEFSNYWNTNFDHIPRALSMILSGKQGSNY
                     SASGIAWLSGLCSQTYGYSFSQVFKFAADTSTNDAGLVGHELGHNFGSNHTHCYNPPI
                     DTCYNGQSGCYSGGTSCPASQTITNNSVSVTNVRGTIMSYCHLLGSCSATNVFHPRVI
                     SERIGPHIDGAVNSCIFPAVTNPSVAAIIPKSGPTSGGTPVVITGTNFLSGASVTIGG
                     SPAAAVIVLSPTTITAVTPARSAGTVSVAVTTPAGAGSLSNSFFYSPPPTSSNFYTLT
                     PCRVVDTRNAAAPRGGPVLGPSTVRTFPVTGVCGIPSSAKAISVNVTIVTPTAAGFLG
                     IFPGNAIPLESSSMNFSAGQVRANNAVLRLATDGAGTIGVQNGSAGNTHFLLDVNGYF
                     Q"
     gene            complement(6293..>6497)
                     /locus_tag="VEL74_25210"
     CDS             complement(6293..>6497)
                     /locus_tag="VEL74_25210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65904.1"
                     /translation="EKGYDPAFGARPLARVLDETVKRPLTDELLFGQLEHGGKVIVDA
                     EGGEMVLRYQPAEQPAEPVPLAH"
BASE COUNT         1010 a         2026 c         2419 g         1042 t
ORIGIN      
        1 cccgcattat gggtgccgaa gggggacggg atgttgtcca ccaggaccac ctcgccggcc
       61 aggtcgacca cgttgcccgg cggcaggacg aagcgggagg cccggttgac gcagacaccg
      121 tccggccgct tgaacgtccg gtagagatag atgaagccgt tgctggggaa gtccgggtcc
      181 acggcgacgc tggagagccc ctgctcccga ttggtgcaga gctcggcggc cgggaacgtc
      241 aaggcggggg tggggagaag cgctcccccc tggtagaccc ggagatcacc aggctgggtg
      301 gtgatcagca tccgcccgtc gggggtaaac cgcaccgacg tcgggctggc gactgtggtg
      361 accagggtgt tggtgtaccc cggagggagg gcggaccgag ccgcccccgt tccgaagccg
      421 acgaagagaa cgaacagaac cggcgccgcg agccgcggcg cgcgcccacg ctcccgtccc
      481 ggcatgtgca cctccaataa gtgttcctca tgctgaggag cacttgctgg aattgcaaaa
      541 atcgcgccgg acagctactc gaagtagccg tagacgtcca ccagcaggtg ggccgatccg
      601 gcgccgccca tggcgcaggt cgccgagaag ccgttgctcc ccagggagac gatggcgttg
      661 ttggcccggg tctgcccggc ggcgaagttg accgtgctgg tgcccgaggc gctcaggccg
      721 gccgggaaga gttggatata gccggatcgg gtgggagcga tcaccgtgac gttgaccgcc
      781 agcgcggcgg ccgacgcggg gatgccgcag gcaccggcca cgtcgaacgt ccgcgtgcta
      841 ccggaggcca gggccgggcc gcccagcggg ccggcagggt cgcgcgtgtc caccacgcgg
      901 cagggcgtca cggtatggaa gtcgaggggg atgctggcgt ccgacgcctc gaaggtggcc
      961 gtgtaggtgg cgggggaggc cggcgtcacg atggcgtggg aggccgcgcc gccgtcggac
     1021 caggaggcga agacccaggt ctggccggcc gcgtcctggg tgggagcgac ggcgttgagc
     1081 cggtagccgt cccacgagat cacagtctgg gggccggtga gggtggtgcc gttgacggtg
     1141 acctgaaggc cggcgggggc ggtggcgaag gtgacgttca ccttccgcgg ctggaagtcc
     1201 cgggtgacgg tggacgagag cccctgggag tcggtggcgg tcaggcgtac ttcgagatag
     1261 ctattggccg ccgcggccaa gtcctcgggg gccggcgtgg tgaacgtgac gttgttgccc
     1321 gctgccggcc cgaagaacgg atgggtgtgg gtatcatggt ggaggacgac cgtccacgat
     1381 agcctgctcg cggggagcgt cccgtcctgc gcgtccgagg cgctacccga gagggtgacg
     1441 ctctggccca cccggaacag ggcgccggcc gccggcgacg agatgaccgg cgcaggcggc
     1501 gtgttgcccg actggatctg cacggtgacc ggggaggaga aagccaagtt gttgtcccgg
     1561 gcgcgcaacg tggcggtgta gttgcccgcc gaggcatagg tgtggttgat ggtcagcgat
     1621 gtggtcgaga cctcgggcgt gccgtcgccg aagctccaga agtaggtcag cgaattgccg
     1681 gtgtcggggt cgctgctgcc ggacgcgttg aaggtcacgg tcagcggcgc cgagccggag
     1741 gtgggggagg ccgacgccac cgcggtgggc gcgttgttgg tcccgggcac gttgtaggag
     1801 atccgccgca cctggccgcc gccggcatag gtggtgtagt agagcgcctg ggcattgccg
     1861 aaggggccga aggtcaggtg gacggcgctg ctgctgccga gaccggtggc gaagtcggtg
     1921 gcggtgtagc cgccccctgc cgccggggtc agcttgaaga tgcggccgca gacgtagtcg
     1981 ctgaacaggt agccgttgtc gtaggccgcc ggccaggtgc cgttggggac gaaggcgccg
     2041 ccggtgatcg agccgcagcc ggtgttgtgg ctgtactcgt ggatcgggtc gaccatgttg
     2101 ggcggcgccg gattgcaggg ccccgaggtg ttccgcggat gcgccccctc acggcagttc
     2161 cagccgtagt cggcccccgc ctgcccgagg tcgatctcct cccaggcccc ctgtccgacg
     2221 tcgttgatga agaaccgggt gccggcggca ttggggtcga acgccatgcg gaacgggttg
     2281 cgcagccccc aggcgaaggt ctcctggcac ttgttgccgg gcgtcgtgct gccgccgacg
     2341 ttgcagcgag ccgtacccgc accctggaac gggttgctcg gggggatacc gccgtccttg
     2401 gtgatgcgca ggatcttgcc ggtcaggacg tgctcgtccc gggcggcgtc gttggactcg
     2461 gcgcagccgc tgtcattcgc gtagtcgcag ccgccgtcgc cgatcgagat gtagaggtag
     2521 ccatcacggc cgaactgcac gtcgccagcg ttgtggttgc cggcgggcga cggcatgtta
     2581 tccaccataa ccagctcggt gatcgagttg atgacgttgt tggcgggcag ggtgaaacgg
     2641 gagacccggt tgacgcagcc gcccggaggg gacggccgct cgaaggtata gaagagatag
     2701 atgaagccgt tcgaggcgaa cgccgggtcc actgcgatac cgagaagccc ctgctcggag
     2761 ctgttgcaca accggttggc cggaatggtc agcgccggcg tcgcgaggag ggcgctcccc
     2821 tggtagacgc gcaggctgcc gccttgggtg gtgatcagca ggcgcccgtc cggggtgaag
     2881 gcgagcgccg tcggggcgcc cacgttggtg accagggcgt cggtgaagcc tgtcggtacc
     2941 gcggcccgga cctcgccggc cgccaggatc aggagagtca ccaggattgc gcggatgctc
     3001 tgcttcgaca tgcctcacct cctcgagtcc tccaggctag cagcttatag agaagagcaa
     3061 aagcgggctc cagcgctaag atggccccat gcctcgccta ctacgtctcc ttctcctgct
     3121 tctctgcatc tccctcccgg ccgccgccca gcctctccgc tgggaggtgg aggggccgcc
     3181 gtctctcgcg ccggtggcgg agcggctccg cggtctggag ccggaccggc tggtgggggc
     3241 gatgcgcctg gtcggcctgg aggacgctgg gccgccgatc cgggtgattg tggcccccga
     3301 gggctcggag gcggcgcggg cggtgccgcc gtgggtctcc ggctacgcgc tgagcgagca
     3361 ggggatcgtc gtcatcctgc cagcgcgcac gccgacctat cccgattctt ctctcccgga
     3421 cctcttccgg cacgagatcg cccacgtgct ggtggcgcga gcggccggag gcggcgaggt
     3481 gccccgctgg ttccacgagg gggtggccat ggtcgccggc ctcacctggg gactggacga
     3541 cgtttcgcgc gccagcctgg ccgtgatggt gcggggcgag gtccccctcg ccaccctgga
     3601 ccgtgccttc gggggcggcc atggcgaggt gcagcgcgcc tatgccatcg cgggcacctt
     3661 tgtgcgcgac ctgctggagc gccacggcca ggacgtcgcc ggccagatcc tggcgggggt
     3721 gcgacagggg ttgccgttcg aggaggcctt cgcccgcgcc accggcgaga ccctggccgc
     3781 ggccgaagcc ggattctggg aacgccagtc gttctggtac cgatgggtgc cgctgctcac
     3841 cagctccgtc accctctggc tcggcatcac cctcctggcc gtctgggcca tccgacgccg
     3901 ccggaagagg gacgcggcgc tacgggcccg ctgggacgaa gaagaaaaac ggctcgccga
     3961 ggtctccctc gacgagccgg tgaattgact gcaggggatc ggacgggatc tgcctactgg
     4021 aaatacccgt tgacgtccag caggaagtga gtgttgccgg cggagccgtt ctggactccg
     4081 atcgtgcccg cgccgtcagt ggcgaggcgc agcacggcgt tgttggcccg cacctgtccc
     4141 gccgagaagt tcatgctgct cgactccagc gggatcgcgt tgcccgggaa gatcccgagg
     4201 aagccggcgg cggtgggggt cacgatggtg acgttcaccg agatcgcctt cgcgctcgac
     4261 gggatgccgc atactcccgt caccgggaag gtccgcaccg tcgaggggcc gaggaccggg
     4321 ccgccacgtg gggcggcggc gttgcgcgtg tcgaccaccc ggcagggcgt cagcgtgtaa
     4381 aagttggacg atgtgggcgg cgggctgtag aagaacgagt tgctcagcga gccggctccc
     4441 gcgggggtgg tcaccgcgac gctgaccgtc cctgcgctcc gggccggcgt gaccgccgtg
     4501 atcgtggtgg ggctcaagac gatgaccgcg gccgccggcg agccgccgat ggtcaccgag
     4561 gccccgctca gaaaattggt gccggtgatc accacggggg tccccccgga agtcgggccg
     4621 ctcttcggga tgatggcggc caccgagggg ttagtcaccg ccgggaagat gcaggagttg
     4681 acggcgccgt cgatgtgggg accgattcgc tcactgatga cccgcggatg gaagacgttc
     4741 gtcgcgctgc aactgcccag cagatgacag tagctcatga tggtgccccg gacattggtg
     4801 accgagacgc tgttgttggt gatcgtctgg gaagccggac agctcgtgcc tcccgagtaa
     4861 cagccgctct gcccgttgta gcaggtgtca atgggcgggt tatagcaatg cgtgtggttg
     4921 gagccgaagt tgtgtccgag ctcgtgcccc accagcccgg cgtcgttggt gctggtgtcg
     4981 gcggcgaact tgaagacctg gctgaagctg tagccatagg tctggctgca caggccgctg
     5041 agccaggcga tgccggaggc gctgtagttg ctaccctgct tacccgagag gatcatcgac
     5101 agggcacggg ggatgtggtc gaaattggtg ttccagtaat tcgagaactc gttgagcttg
     5161 gcactatcgg cgctgccggt gccggactgc gcgtagggat cggccgtggt cgagggacgc
     5221 aggatggtgt gcccctgcaa gagccggatc ttgaggtccc gctcgtagaa cacgttcatt
     5281 ttggcgatca ggttggcgat gtagctcgtg gcgctggccg tgttgttgga gaacttcaag
     5341 gacatgagct cgttgtcggt gtcgacggcg atcacggcgg tgtggatcga gctgagcacc
     5401 ttctccgaga cggagccgag gctggacggc gccgaggcct tagtcagggg ctgcggcaga
     5461 tcctcctcgc cgcaggtgaa acgggggccg tcagtctcgc cgccggttaa tgccttgtcc
     5521 ccggcctcga acgcctccga agaagcaacc atgtgctgcg cctgcggccc atccgccggc
     5581 agcgggcgca gttcctggat gccgtcgatc gactgcgaga agccccggac ctcgccggtc
     5641 gccgggtcca ccgacaggaa gacgcggacg tcctcgtcca cctccgccgc cccccagtag
     5701 aagaccaggt tcgagcgcgg cacctcgacc agccgcttgc ctcggacctc gtagatcttc
     5761 gcgtcgggcg cgtagatctc ccgccggtgc agaacgacat ccgccctcag ctcgggggcg
     5821 atcggccagt ctcccagccg cacctcggcg tcgggaggga tggagaggag gagcggcgcg
     5881 actccttgat ccagggtcac cacctcggcc gggctctgca ggtccggagc gaccgccacc
     5941 tccccgggac cggtggcgcg gatcgccggg atccggaaat caggctgtgc cgttagagct
     6001 gcgggagccg ccaacgcggc ggcgaacacg caaatcccct ggagaagtcg accgatacgc
     6061 accatccgta tgcctccctg tttgggcgat gagaaatagc aaacaatcga tggattgcca
     6121 gcattcccag ctccgtcatc tcagaccacc gatccaacaa ttgggctgag tttgtccaag
     6181 gcacggggcg tgccaggagg cgagaggctg caaacctttg aaaagagaag gggggctccc
     6241 ttccagggcg acaaaagctg tcaccccgga aggggagggc tgtcagggat tgtcagtgag
     6301 ccagcgggac cggctcggcg ggctgctccg ccggctggta gcgcaggacc atctcgccgc
     6361 cctcggcgtc gacgatgacc ttgccgccgt gctcgagctg gccgaagagc aactcgtcgg
     6421 tgagcggccg cttgaccgtc tcgtcgagca cgcgcgccag cggccgggcg ccgaaggcag
     6481 ggtcgtagcc tttctcg
//
LOCUS       DASPJI010000894        20462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_171054, whole genome shotgun sequence.
ACCESSION   DASPJI010000894 DASPJI010000000
VERSION     DASPJI010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 20462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20462)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20462
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171054"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..746
                     /locus_tag="VEL74_25215"
     CDS             99..746
                     /locus_tag="VEL74_25215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HYG65905.1"
                     /translation="MSAPLPSISREDFRRLLGRHSPEPLDGRALDACYVHYQELALWN
                     RRLSLIGPGTADEVVARHFGESLAALPLIPPGARTAVDVGSGAGFPGLVLAAAKPGME
                     TTLVEARERKWSFLQSVARKASLPCRCLNARVQLPLPAGLPDCIDVLTARALKLPPEI
                     LGAVARRMGPEGRVLLWVGEKDPEIPPDLAPADSVKLEGSERRRILCLRPLEPER"
     gene            805..1563
                     /locus_tag="VEL74_25220"
     CDS             805..1563
                     /locus_tag="VEL74_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HYG65906.1"
                     /translation="MTMILAIANQKGGVGKTTTAINFGAGLGALERKVLLVDCDPQGN
                     ASRGLGVKPEPPHLYHALTGEVPLADTIRPSGFPNLDLVPAQRDLVGVEVEFVGEPAW
                     ERRLRSVLEQVEGRYDTILLDCPPSLGHLTVNALVAADGLLVPLQCEYFALEGVSELV
                     STVNRIQAGLNPKLSIAGILLTMYDDRTNLSRDVAEEIRRHFAGKVFDTVVPRNIRLA
                     EAPSHGLPIFQYDIKSRGAEAYLALAREFLRRAA"
     gene            1560..2441
                     /locus_tag="VEL74_25225"
     CDS             1560..2441
                     /locus_tag="VEL74_25225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HYG65907.1"
                     /translation="MTVGSSKKRGLGRGLDALIVKEEPTEAAAAARTLPVDSLRPNRL
                     QPRTHFQEEAIQELAESIRAQGVIQPLVVTPEEDGSFSIIAGERRWRAARRAGLESVP
                     VVVREGLDDRTRLEIALVENLQRSDLNPLEEAEAYQLLQEKFGLSQEEVAVRVGKGRP
                     TVTNSLRLLKLPEEVRDLLREGQLTAGQARPLLGLATREEQIELAERAVRENLSAREI
                     ERLTAAPREEQEPKKKPARTVDVHTAAAQENLTRRLQTRVEIRRKGKGGLLQVHFHSE
                     EELMRLYDVLMEREEAP"
     gene            2438..2845
                     /locus_tag="VEL74_25230"
     CDS             2438..2845
                     /locus_tag="VEL74_25230"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HYG65908.1"
                     /translation="MIFKSEVKQSDLNGFLDEGSHLQGELRFETSFRIDGKFTGQVVS
                     QGDLIIGAGGEVDGDLAVGQVFVSGTVKGTIRAARRIQITPTGKVVADLETPVLIVED
                     GAFFRGNCAMEREVKVAESPKLVAPKVKAAQAG"
     gene            complement(2865..4583)
                     /locus_tag="VEL74_25235"
     CDS             complement(2865..4583)
                     /locus_tag="VEL74_25235"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYG65909.1"
                     /translation="MRLPRPGWLDVVWGGLLPGALAGVHLAGLIFFLNPRIPFTPGPV
                     ARGILFYSGLLGAASLLLHLPFLWGRPRRARRWLPWGLTLVLGGAAALDWTHASHYAY
                     YLPAGINDRLLKTALWLTLGALIAFYTALLHSLHRRRYGWRSRYGFALMALLSVYATV
                     ERREAFRPQPVEPPPPIAVEAGQRPRLWVVGLETATLDAILPLAGQGRLPFLAGVLEQ
                     GAYGRLETFSPSWREAVWTTLATGKYPHKHGVKGSRSYPASWIAPGAEIILVPVGISF
                     RRWGMPWTGPERGGHPRRALALWEILPRLEIPAATLAWPASAPAGPGPVYSLTDRFFS
                     DPRDRAAAKPQDLAARALLFRVPPDEIGPSERARFGPDPRRSLLEALAGDLWRESISF
                     FLLEQHPEVEATFVTLPGLREVSRRDFGGFAAVQFEGAQEEEARQAAERVAGYYAELD
                     EYLAEVWSRLPEPKVLAVVSAHGVEPRPGALADAFGPRDLGGDFNDAPDGVLLLHGSA
                     GTGIRPRALLTGARAVDLVPTLMYALGFPVARDLDGQVLTAAFDERYLARHPLTFLPT
                     YEGLQE"
     gene            complement(4603..4971)
                     /locus_tag="VEL74_25240"
     CDS             complement(4603..4971)
                     /locus_tag="VEL74_25240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65910.1"
                     /translation="MQRRTAERGEGNLGCILWLVLLGVGVLVAFKTIPVKVQSAELYD
                     YMVELTKFAASSTPQQLEKQILNRARELDLPLDKDSVEVRKGGDRIQMEARYMVPIEF
                     PGYTYEWQFDHEIDRPIYIF"
     gene            complement(5002..5700)
                     /gene="lepB"
                     /locus_tag="VEL74_25245"
     CDS             complement(5002..5700)
                     /gene="lepB"
                     /locus_tag="VEL74_25245"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HYG65911.1"
                     /translation="MQTEKSLLREYLEALLIAVVFATFARTYVVQAFKIPSGSMERNL
                     LIGDHILVNKFVYGPTATDLERKLLPVRPLRRGDVVVFKYPEDPERDFIKRCMALPGD
                     TLEIADKAVHINGQRIADDSYVHHSDSVSYPRSLFLSPNRRRDNFGPFTVPPGEYFCM
                     GDNRDNSNDSRVWGTVPEDHVKGRAFMVYWSFESEGEDIEWPGLWGKLKQLSHVALNF
                     FSRTRWERTFRIVR"
     gene            complement(5708..6397)
                     /gene="lepB"
                     /locus_tag="VEL74_25250"
     CDS             complement(5708..6397)
                     /gene="lepB"
                     /locus_tag="VEL74_25250"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HYG65912.1"
                     /translation="MTKERSVLREYLEALLIAVIFLRFTQTFVTQTFYIPSGSMENTL
                     LIGDHLFVNRFIYGPAATELERKLLPLRPVQRGDIVVFRSPENPQLDLVKRCIGLPGD
                     EIRLIDKELYLNGTAVDDASYVIHTDTYSDDFDPHRQRRDNFGPYTVPAGHYFCMGDN
                     RDNSNDSRFWGPLPAHMVKGRSLFIYWSYGGETSDGQWRGIGDKLRDLGETAVGFFTR
                     TRWERTFRLIR"
     gene            6568..8427
                     /locus_tag="VEL74_25255"
     CDS             6568..8427
                     /locus_tag="VEL74_25255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65913.1"
                     /translation="MPTPPRLTWKHRLLLIGFGIFLVVLLELGLRLAGYGGRVPLLNP
                     LPLREGTGRLYEVHPKAAQIFFARSGPGGMSLVGSHRRDLVVLPKPPDVVRVVFVGAS
                     TVEGFPMPRNLTSARFLEAMLQEELAGKRVEVVNLGVAAVASFPIRKLAVEALETMSP
                     DLLLVYEAHNEFFGASGMASFQSMGRSVKAMQAVYFVRQLALAQVVDDLQGALRRPAT
                     PGEEGERPDLIRLMAAADEIPPDGELHAAARRSLEDNFRAIVQEARDRGVPVVLSTVV
                     SNERDLVPLGGSEPADAAYAGARALEREGRRAEAAEQYRRARDLDTLPWRASRDKNRV
                     LRDLAADEEVPLADCEAAFAAASSSTGGATTWELFVDHVHPSLRGQALLAETFFQTIV
                     RHRLLPVDPSGAAAPTDWRQGAARLGAHPLELYLVAHKMAALFRVPPIGRNNEAAARR
                     FAGLVDRLRGGADPVDAEAIRLWELGSAQAGFALPISYFGGVSALRMGEPQRATVYLH
                     AAVENAFPWSDERSAARLLGLIAAVRAGAELSSIRPRLTSALDEARQVATLPGQPTAL
                     LALSLAGLSALAGDPAERSRWESLARDRTAGAPPWERAFLRELPDPGALTPLP"
     gene            8821..9195
                     /locus_tag="VEL74_25260"
     CDS             8821..9195
                     /locus_tag="VEL74_25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HYG65914.1"
                     /translation="MNRTYRFASSVTLVLFFLLLSALPGTAAEGKRAVNINSADASQL
                     ALLPRIGPSVAQRILEFRKQNGPFKSAEDLMLVRGIGEKTFALIKPYVAVSGETTLKE
                     KVKGAKPSSSKEGGKDSKEGSR"
     gene            9253..9849
                     /locus_tag="VEL74_25265"
     CDS             9253..9849
                     /locus_tag="VEL74_25265"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HYG65915.1"
                     /translation="MGSREKGFTVVEASIYGAVLAAAMLVTGPPLLQLSGDLRLRAAA
                     AEAAAVLRSTRFLAVRHSAHVAVRFGAWAGGPITYAIYRDGDGDGVLSRDITRGVDPE
                     LSPPRPLGDGHAIRFGFPPGLRVRDPGDPRVWMKTEDPVRFNQSNMASFGPLGESTPG
                     SLYLTDGDRRLVVVRVFGRTGKIRVLSYDFDTQKWLQR"
     gene            9937..10326
                     /gene="cutA"
                     /locus_tag="VEL74_25270"
     CDS             9937..10326
                     /gene="cutA"
                     /locus_tag="VEL74_25270"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HYG65916.1"
                     /translation="MRGIVVVTTVGTEEQAYLIARELVGRRQAACVNVMPGVRSIYRW
                     KGKICKDGELMLIIKTLEGEFEGVEATIKELHSYELPEILSFEVAQGERNFLEWIRSS
                     VDKDADFSDEEEDEIAYAAADPDDEAF"
     gene            complement(10349..11515)
                     /locus_tag="VEL74_25275"
     CDS             complement(10349..11515)
                     /locus_tag="VEL74_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HYG65917.1"
                     /translation="MKGDPKNLGFSTRAIHAGQSPDPRTGAVAVPIYQTSTYVQEGVG
                     EGAFYEYARVQNPTRAALEENVASLEKGVSGHAFASGMSAIACLMTLVRTGDHVVVSQ
                     NVYGGTYRYFTRLLEPYGVTFSWVDTSDLRAVEAAIRPETKMLYIETPTNPTMEITDL
                     RAAADLGHSRNLLVAVDNTFLSPYLQRPLELGCDFVLHSTTKFLNGHSDSIGGALVAA
                     RPEHSEWFAFVQKSEGAILSPFDSFLVMRGIKTLAVRMERHEQNARRVVDFLVGHPKV
                     KRVLYPGLPDFPGHEIQKRQASGFGAMISVEMGSYESGKAFLDGLRVISLAESLGGVE
                     SLASHPASMTHASVPAERRALIGLSDGLVRFSIGIEDIDDLLADLDQALAGVPA"
     gene            11613..12722
                     /gene="mtnA"
                     /locus_tag="VEL74_25280"
     CDS             11613..12722
                     /gene="mtnA"
                     /locus_tag="VEL74_25280"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HYG65918.1"
                     /translation="MSEPRTDSTTPAGPEQVFSPMRWEDGRLLLLDQTRLPTEEAWLE
                     CETPEQVADAIYRLAVRGAPAIGVAAAFGLVLGLQAVPAPEGREALARRFEEVSRLLG
                     STRPTAVNLRWALERGREVFEANMASGPEAATRALLAWAHELHAEDVRVNRRIGDHGA
                     ALFGSGARVLTHCNTGSLATAGYGTALGIIQSAWRDGKLGMVWVDETRPLLQGARLTA
                     WELKKLGIPFRLVTDSSAGTLMAQGMVDRIVVGADRIAANGDTANKIGTYTVAVLAHR
                     HGVPFYVAAPLSTIDRATATGREIPIEERKSGEVTEVFGTRIAPDETPALNFAFDVTP
                     SELITAIVTEVGVLEPPYTESIAAAFGTAVGRAKS"
     gene            complement(12761..13114)
                     /gene="ssrA"
                     /locus_tag="VEL74_25285"
     tmRNA           complement(12761..13114)
                     /gene="ssrA"
                     /locus_tag="VEL74_25285"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(13191..13652)
                     /locus_tag="VEL74_25290"
     CDS             complement(13191..13652)
                     /locus_tag="VEL74_25290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65919.1"
                     /translation="MAEHVIELPDPPEATLSAVARAAEMWGGELERQGGGGRLHIPVL
                     AGLRRGFVAGPLAVEPTTEGSRVVFRPDDQNLYVHIPSLVILLISVAGAVLTVLWPFY
                     PRLLPLAPFGVLLALGGWFLVLTRLRTSGPDEFLEMAAGLLGEEEAARGKT"
     gene            complement(13665..14648)
                     /gene="dusB"
                     /locus_tag="VEL74_25295"
     CDS             complement(13665..14648)
                     /gene="dusB"
                     /locus_tag="VEL74_25295"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HYG65920.1"
                     /translation="MVQVGNVQVDPPLWLAPMAGVTDRDFRLIVRRIGGVGVVSMEFI
                     SSRAIVSGNRRTRELMYFAEEERPLAIQIYGSDVATMREAALVVEELGADICDINMGC
                     PANKVLKGCAGAALMGDLELAEQIVRAVKGVLTIPLTVKFRLGLDDCRRNYLELGRLC
                     EANGVAAVAMHARTARQMFTGEADWSHLARLKEALSIPVIGNGDVREPEDALRMMAET
                     GCDGVMVGRGATRNPWLFRQIAARMSGSGFSEPTLEDRRDLILSHFRMVAEREHSTFA
                     LHKLRKFTGWYTHGLPNGRRLRQEINQIPDVPSFLAAVESFFDDTGEEKAA"
     gene            complement(14697..14972)
                     /locus_tag="VEL74_25300"
     CDS             complement(14697..14972)
                     /locus_tag="VEL74_25300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65921.1"
                     /translation="MRLRPSPRAALVAMIVGAVLHTAAALWAWQTWGVFGRGNVLTWM
                     DLPVSLLYLHLDGPTLLTWSLIAGGLQWAAIAAGLTLLLGRSARHRA"
     gene            complement(14969..15697)
                     /locus_tag="VEL74_25305"
     CDS             complement(14969..15697)
                     /locus_tag="VEL74_25305"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HYG65922.1"
                     /translation="MLSLMQMDWIGVAEGRFWMGGGPRDNENPRHEVWVPAFRLARAP
                     VTREEYQRFLDATGHPAPPFWSEAAFSHPRMPAVGPSWEDAAAYCAWLAGELEDSGDR
                     PRLPSEAEWELAARAGRDVLYPWGDEPPESLPDYDRRWARGPEPVDAYPSPHPLGFLG
                     LGENVHEWCSDWYDAGYYAVSPAEDPRGPAAGTRRSSRGGSWRHEIKASRCAARSAIP
                     PHLRYSDYGFRLAADAPSGAGRAA"
     gene            15858..16580
                     /locus_tag="VEL74_25310"
     CDS             15858..16580
                     /locus_tag="VEL74_25310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG65923.1"
                     /translation="MAWYKEWFGEDYLDLYSHRDESEAEAHMDFVERHLDTGHRPRAV
                     LDLACGAGRHTAALRRRGYRTLGVDLSLTLLAHMHRLPRVAGDMRCLPFTAGSFDWVL
                     NFFTSFGYFERERENFQVLEEIVRLLTPGGRFLIDLLNPGYVLARLEPRETQEHDGRR
                     VEIERWYDPAKLRINKRIRVLEPGRPARSFVESVRAYRAEEVTIGLRWAGLEVSNLFG
                     GFQGEPYQNDSERLIIVGYKPA"
     gene            16537..18189
                     /gene="bshC"
                     /locus_tag="VEL74_25315"
     CDS             16537..18189
                     /gene="bshC"
                     /locus_tag="VEL74_25315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HYG65924.1"
                     /translation="MTANASSSSATSPPETPRGGEPVATALPPLPSAWRAGRDLDLLA
                     PLRLLTGAEPPSGPAPVLAGAAAVARRELAEALAVSNRAYGHEGADALARRLADPATR
                     VIVTGQQPGLLGGPLYTFAKAVAAARWAAALEVAGQPAVAVFWVATEDHDWAEVSAAT
                     ILASGGPRTFDLGPDPAPLTPVGMRTLGDGVTGVLDEIMAAVPNERYAEWLRTVARWY
                     RPDARFGEAFCRLLAHMLGDRCPLLLDAMHPALKAAQRPWLRRLVERRREAEEALAAR
                     EAEITGRGYPLQVAPQRGVSPLFLLHRGERRRVEWRGEAGYALRGRDDAGGTVTDLLR
                     VIDENPGVVTPGVLARPAIQDAVLGSCLQVMGPGELSYLAQVAAVYPVLEVEAPWTSL
                     RPQTLVLESHQAERLAEAGITLADLLGDRQRLDRALASSEDGEPVAAVRARVEQALDD
                     LKASSLAADPNLERPLEKTREQILRALDLFAEKTTGALARKNEVRSRRVEQLREACLP
                     LGKLQERVVCAAHFQGKHGSRFAESYWEQMGLDPGTLQVISP"
     gene            18214..18954
                     /gene="bshB1"
                     /locus_tag="VEL74_25320"
     CDS             18214..18954
                     /gene="bshB1"
                     /locus_tag="VEL74_25320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HYG65925.1"
                     /translation="MKLDVLAIGSHPDDVELGCGGTLALMARQGRRVGILHLTGGERS
                     TRGTPAERRAEAEAAAQALGAAELAFLDCGDASFRYGEAEEDALIGILRVWRPELVLG
                     PTPRDRHPDHGRAHELVHAACFYSGLASRGERDVKPHRPAAVFSYMQHDLFEPSFIVD
                     VTAVWETKLQALAAYRTQLYQRTAGEREGKDTAPVTKVSTREFALANEGRSRHFGLLI
                     GAEFGEPFWSRLPLAVADPMTILPGGVR"
     gene            18951..20075
                     /gene="bshA"
                     /locus_tag="VEL74_25325"
     CDS             18951..20075
                     /gene="bshA"
                     /locus_tag="VEL74_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HYG65926.1"
                     /translation="MKIGISCYPSYGGSGIVATELAMALADGGDDVHIISYAFPSRLS
                     ILNPRIFFHEVVTPHYPLFEYPPYSLALATKMVEVGRHHQLDLLHVHYAVPNAVSAIL
                     ARQILAPQQLPVVTTLHGTDVTLVGNDPNYLETTRWGIVESDGVTAVSESLRQTTVEQ
                     LGIHKRIDVIPNFIDPARFESARHSPGARRWAKSNERILIHISNFRPVKRVLDVVEIF
                     DRVRREVPCRLLMVGDGPDRAKIEQRCRECRLCDDITFVGSLPLIEEVLVGGDLFILP
                     SETESFGLAALEAMACEVPVITTQAGGLPEVVVEGETGHLRPVGDVDGMAEAAVRLLQ
                     DEPRRRAMGEAGRRRAVEIFSQDAVVARYRQLYDRVMQRS"
     gene            20082..>20462
                     /locus_tag="VEL74_25330"
     CDS             20082..>20462
                     /locus_tag="VEL74_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010122199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HYG65927.1"
                     /translation="MSAYPAPGGEARAEIREKGSRFLAVLGPAAGEEEAKARLADLEK
                     LYPDATHHCWAWRLGWPPRERSSDAGEPGGTAGVPILQVLRGAGLSDVMAVVVRWFGG
                     VKLGKGGLARAYAGAAREALHELPL"
BASE COUNT         3044 a         7224 c         7273 g         2921 t
ORIGIN      
        1 cgacggcccc atgtttcacg tgaaacatgg ggccgtcgag tctctgggcc atgttccacg
       61 tgaaacattc ctctcccaat ctccgcaagg aggtctgaat gtccgctccg ctcccctcga
      121 tctcccgcga agacttccgg cgcctcctgg gccgccactc ccccgagccg ctggacggcc
      181 gagccttgga tgcctgctat gtgcactacc aggagttggc gctgtggaac cggcgcctct
      241 ctctcatcgg gcccggcacc gccgacgagg tcgtcgcccg ccacttcggc gaatcgctgg
      301 cggctctgcc gctgatcccg cctggagcgc ggacggcggt cgacgtaggg tccggcgccg
      361 gcttcccagg cctggtgctg gcggcggcga agcccggcat ggagacgacg ctggtcgaag
      421 cccgcgagcg caagtggtcg ttcctgcagt cggtcgcccg caaggcctca ttgccctgcc
      481 ggtgcctgaa tgctagagtg cagcttccac tgcctgccgg cctgccggac tgtatcgacg
      541 tcctcaccgc ccgggccctg aagctccctc cggagatcct cggcgccgtc gctcggcgta
      601 tggggcccga ggggcgcgtc ctgctctggg tcggggagaa ggaccccgag atcccgccgg
      661 atctcgcgcc ggcggacagc gtgaagctcg aagggagcga gaggcggcgc atcctgtgcc
      721 tgcgccccct tgaaccggaa cggtaggact ctaggattgc ggaggcccgc gcaggcgact
      781 ggcggtggac tcctgggaac ctgaatgacc atgatcctgg cgatcgccaa ccagaagggt
      841 ggagtcggca agaccaccac cgccatcaac ttcggcgccg gcctcggcgc gctggagcgc
      901 aaggtgctgc tggtggactg cgatccccag ggcaacgcct cccgcggcct cggcgtcaag
      961 cccgagcccc cgcacctcta tcacgccctt accggtgagg tccccctggc cgacaccatc
     1021 cgtccctcgg gcttccccaa cctcgacctc gtacccgcgc agcgcgacct cgtgggcgtc
     1081 gaggtggagt tcgtcggcga gccggcctgg gagaggcgcc tgcgctcagt gctcgagcag
     1141 gtcgagggcc gctacgacac catcctcctg gactgcccgc cctccctcgg ccaccttacg
     1201 gtcaacgccc tcgtggccgc ggacgggctc ctcgtccccc tgcagtgcga gtacttcgct
     1261 ctggaagggg tgagcgagct ggtgtccacc gttaatcgca tccaggcagg gctgaacccc
     1321 aagctctcca ttgccggcat cctgctgacg atgtacgacg accgcacgaa tctctcgcgc
     1381 gacgtggccg aggagatccg ccgccacttc gccggcaagg tcttcgacac cgtggtcccc
     1441 cgcaatatcc ggctcgccga ggcgccgagc cacggcctgc ccatcttcca gtacgacatc
     1501 aagtcgcgcg gtgccgaggc ctacctggcg ctggcgcgag agttcctcag gagggcggcg
     1561 tgaccgtcgg cagcagcaaa aagcggggcc tcggccgggg cctcgacgcg ctcatcgtca
     1621 aggaggagcc gacggaggcg gccgcagcgg cccgcaccct gccggtggac agcctcaggc
     1681 ccaaccggct ccagccccgc acccacttcc aggaggaggc gatccaggag ctggccgaat
     1741 ccatccgcgc ccagggggtg atccagccgc tggtggtcac ccccgaagag gacggctcgt
     1801 tcagcatcat cgccggcgag cgccgctggc gcgccgcccg ccgcgcgggg ctcgaatccg
     1861 tgccggtggt ggtgcgcgaa gggctcgacg accgcacccg cctggagatc gccctggtgg
     1921 agaacctcca gcgcagcgac ctcaacccgc tcgaagaggc cgaggcctac cagctgctgc
     1981 aggagaagtt cggcctgtcg caggaagagg tcgcggtgcg cgtcggcaag ggtcgcccca
     2041 ccgtcaccaa ctccctgcgc ctgctgaagc tccccgagga ggtgcgcgac ctgctgcgcg
     2101 aggggcagct caccgccggc caggcccggc cgctcctcgg gctcgccacg cgcgaggagc
     2161 agatcgagct cgccgaaagg gcggtgcgcg agaacctctc cgcccgcgag atcgagcgcc
     2221 tcaccgctgc gccgcgggag gagcaggagc ccaagaagaa gccggcgcgg acggtcgacg
     2281 tccacaccgc cgccgcccag gagaacctga cccgccgcct ccagactcgc gtcgagatcc
     2341 ggcgcaaggg caagggcggg ttgttgcagg tccatttcca ttccgaggag gagctgatgc
     2401 ggctctatga cgtgctgatg gagcgggagg aggcgccgtg atcttcaagt ccgaggtcaa
     2461 gcagagtgat ctcaacggct tcctggacga agggagccat ctccagggcg agctccggtt
     2521 cgagacttcg ttccggatcg acggcaagtt caccggccag gtggtctccc agggggacct
     2581 gatcatcggc gccggcggcg aagtcgacgg cgacctcgcg gtcggccagg tcttcgtctc
     2641 gggcacggtt aaagggacga tccgagccgc gcggcggatt cagatcacgc cgaccggaaa
     2701 ggtggtggcc gatcttgaaa cgcccgtcct gatagtggag gatggcgcct tcttccgggg
     2761 caactgcgcc atggagcggg aggtcaaggt cgccgagagc ccgaagctgg tggcccccaa
     2821 ggtcaaggcc gcccaagccg gataaaccag ggtgagacct cgccctactc ctgaagcccc
     2881 tcgtacgtcg ggaggaaggt cagggggtgg cgggcgaggt atctctcgtc gaaggcggcg
     2941 gtcagcacct gcccgtcgag gtcccgtgcc acggggaagc cgagggcgta catcaacgtc
     3001 ggcaccaggt ccacggcccg cgcgccggtg aggagcgccc gtgggcggat ccccgtgccc
     3061 gcggagccat ggagtagcag cacgccgtcc ggggcgtcgt tgaagtcgcc gccgaggtcg
     3121 cgcggcccga aggcgtccgc cagggcgccc ggccgcggct ccacgccgtg ggccgacacc
     3181 acggccagga ccttgggctc gggcagccgg ctccagacct ccgccaggta ctcgtcgagc
     3241 tcggcgtagt agcccgccac acgctccgcc gcctggcgcg cctcctcctc ctgagcccct
     3301 tcgaactgca ccgccgcgaa gccgccgaaa tcccgccgcg agacctcccg caggccgggg
     3361 agcgtcacga aggtggcctc cacctcgggg tgctgctcca ggaggaagaa ggagatcgac
     3421 tcgcgccaga ggtcgccggc cagcgcctcg aggagggacc gccttgggtc cgggccgaag
     3481 cgcgcccgct cggaagggcc gatctcgtcc ggcggcacgc ggaacagcag cgcccgcgcc
     3541 gcgaggtcct gaggcttggc ggcggcgcga tcccgggggt cggagaagaa gcggtcggtg
     3601 aggctgtaca ccggcccggg tccggcgggc gcggaagccg gccaggcgag ggtggcggcc
     3661 gggatctcca ggcgggggag gatctcccag agcgccagcg ctcgccgggg gtggccgccg
     3721 cgctccggcc ccgtccacgg cataccccat ctacggaacg agatccccac cggcaccagg
     3781 atgatctccg cgcccggcgc gatccaggac gcggggtagc tccgcgatcc cttgaccccg
     3841 tgcttgtgcg ggtacttgcc ggtggccagc gtcgtccaga ccgcctcgcg ccaggagggg
     3901 gagaaggtct ccaggcggcc gtaggcgccc tgctccagga cgccggcgag gaagggcagc
     3961 cgtccctgtc cggcgagagg gaggatggcg tcgagggtcg cggtctccag gcccaccacc
     4021 cagaggcgtg gccgctgtcc ggcctccacg gcgatggggg gcggcggctc cacgggctga
     4081 ggccggaacg cctcgcggcg ctcgaccgtg gcgtagaccg acagcagcgc catcaaggcg
     4141 aagccatagc ggctgcgcca gccataacgg cgccggtgaa gggagtggag gagggcagtg
     4201 tagaaggcga taagcgcccc gagggtcagc cagagggcgg tcttcaggag ccggtcgttg
     4261 atgccggccg gcaggtagta ggcgtagtgg gaggcgtggg tccaatccag ggccgcggcg
     4321 cctccgagga ccagggtgag gccccagggg agccagcgcc gcgcccgccg cggccggccc
     4381 cagaggaacg gcaggtggag gaggaggctg gcggccccga gcaggccgct gtagaacagg
     4441 atcccccgcg ccaccggtcc cggggtgaac gggatccgtg ggttgaggaa gaagatcaac
     4501 cccgccagat ggacgccggc gagggcgccg ggcaggagcc ctccccatac gacgtcgagc
     4561 caaccggggc ggggaagacg caccgccgct cctcctcccc ggtcagaaga tgtagatggg
     4621 ccggtcgatc tcgtgatcga attgccactc gtaggtgtag cccgggaact cgatcggcac
     4681 catgtagcgg gcctccatct ggatccggtc gccccccttc ctcacctcca cgctgtcctt
     4741 gtccagcggc aggtcgagct cccgcgcccg gttcaggatc tgcttctcca gctgctgcgg
     4801 cgtgctggag gcggcgaact tggtgagctc caccatgtag tcgtagagct cggcgctttg
     4861 caccttgacc gggatggtct tgaaggcgac caggacgccc acgcccagga gcaccagcca
     4921 gaggatgcac ccgaggttcc cttcaccgcg ctcggcggtt ctgcgttgca tgactccctc
     4981 cttcgaaact tggcgaagag tctaacgcac gatgcggaag gtacgctccc atcgagtgcg
     5041 ggagaagaag ttgagggcta catgggagag ctgcttgagc ttgccccaga gccccggcca
     5101 ctcgatgtcc tcgccctcgc tctcgaagga ccagtagacc atgaaggcgc gccccttcac
     5161 gtggtcctcg ggcacggtgc cccagacacg ggagtcgttg gagttgtccc ggttgtcccc
     5221 catgcagaag tactcgccgg gggggacggt gaacggcccg aagttgtcgc gccggcggtt
     5281 ggggctgagg aacaggctgc gcggatagct gacggagtcg gagtgatgga cgtaggagtc
     5341 gtcggcgatc cgctggccgt tgatgtggac cgccttgtcg gcgatctcca gggtgtcgcc
     5401 gggcagtgcc atgcagcgct tgatgaagtc gcgttccggg tcctcggggt acttgaagac
     5461 caccacgtcg ccgcggcgca gagggcggac ggggaggagc ttgcgctcga ggtcggtggc
     5521 cgtcgggccg tagacgaact tgttgaccag gatgtgatcc ccgatcagca ggttccgctc
     5581 catcgagccg gaggggatct tgaacgcctg cacgacgtag gtgcgggcga aggtcgcgaa
     5641 aacgaccgcg atgagcaggg cctccaggta ctcccggagc agggacttct cggtctgcat
     5701 ggggaggtta ccggatcagc cggaaggttc tctcccaccg cgtccgggtg aagaagccga
     5761 cggcggtctc cccgaggtcc cggagcttgt cgccgatccc ccgccactgg ccgtcggagg
     5821 tctcaccgcc gtacgaccaa tagatgaaga gcgagcgccc cttgaccatg tgcgcgggca
     5881 gcggccccca gaaccgggag tcgttggagt tgtcgcggtt gtcccccatg cagaagtagt
     5941 ggccggcggg gacggtgtag gggccgaagt tgtcgcgccg ctggcggtgg gggtcgaagt
     6001 cgtcgctgta ggtgtcggtg tggatcacgt agctcgcgtc gtcgaccgcc gtgccgttga
     6061 ggtacagctc cttgtcgatc aggcggatct cgtcgccggg caggccgatg cagcgcttga
     6121 ccaggtcgag ctgcgggttc tccggcgacc ggaagacgac gatgtcgccg cgctgcaccg
     6181 ggcgtagcgg cagcagcttg cgctcgagct cggtggccgc agggccgtag atgaaccggt
     6241 tgacgaagag gtggtcgccg atcagcaggg tgttctccat cgacccgctg gggatgtaga
     6301 acgtctgcgt cacgaaggtc tgggtgaagc gcaggaagat caccgcgatc agcagcgctt
     6361 ccaggtactc ccgcaggacg gaccgctcct tcgtcatagc gggctaatct acccgaaagg
     6421 ctcccttgaa gctaccggag ccctccgccc ggtggtgcgg agggtccgtg ctcggctcct
     6481 tcgggtgcgc cccgccccca cccgccccgc gcgtccagtc gcctgcgccg gacccctccg
     6541 caccaccccc cgccctatac ttcccggatg ccaacccccc cgaggctcac ctggaagcac
     6601 cggctgctgc tgatcggctt cgggatcttc ctggtggtgc tgctggagct ggggctgcgc
     6661 ctcgccggct acggcggccg ggtgccgctg ctcaatccgc tgccgctgcg ggaggggacg
     6721 ggacggctgt acgaggtcca ccccaaggcg gcccagatct tctttgcccg cagcgggccg
     6781 ggggggatgt cgctcgttgg cagccaccgg cgcgacctgg tcgtcctccc caagccgccg
     6841 gacgtggtgc gcgtggtctt cgtcggcgcc tccaccgtcg aggggttccc catgccgcgc
     6901 aacctcacga gcgctcgctt cctcgaggcg atgctccagg aggagctggc gggaaagcgg
     6961 gtggaggtgg tcaacctcgg cgtggcggcg gtggcgagct tccccatccg caagctggcc
     7021 gtcgaggcgc tggagacgat gagcccggac ctcctgctgg tctacgaggc gcacaacgag
     7081 ttcttcggcg cctcggggat ggcctccttc cagtccatgg gccgcagcgt caaggccatg
     7141 caggcggtct acttcgtccg ccagctcgcc cttgcccagg tggtggacga cctgcaaggg
     7201 gcactgcggc gccccgccac cccgggagaa gagggcgagc ggccggacct gatccggctc
     7261 atggcggcgg cggacgagat cccgccggac ggcgagctgc acgccgcggc gcgccggagc
     7321 ctggaggaca acttccgggc gatcgtccag gaggccaggg accgcggcgt gccggtggtc
     7381 ctttccacgg tggtgagcaa cgagcgggac ctcgtccccc tgggaggctc ggagccggcg
     7441 gacgcggcct acgccggcgc ccgggctctg gagagggagg gccggcgcgc cgaggccgcc
     7501 gagcagtatc gccgtgcgcg ggatctcgat accctcccct ggcgggcctc gcgagacaag
     7561 aaccgggtcc tgcgcgatct cgcggcggac gaggaggtgc ccctcgccga ctgcgaggcc
     7621 gccttcgccg cggcgtcgtc ttctacggga ggcgccacca cctgggagct gttcgtcgac
     7681 cacgtccacc ccagcctgcg ggggcaggcg ctcctcgccg agaccttctt ccagaccatc
     7741 gtccgccatc gcctgctgcc ggtggacccc tccggcgccg ccgcgccgac ggactggcgg
     7801 caaggcgccg cgcggctggg cgcccatccg ctcgagctct acctggtggc ccacaagatg
     7861 gccgcgctgt tccgggtgcc ccccatcggc cgcaacaacg aggccgccgc gcggcgcttc
     7921 gccggcctcg tcgatcgcct ccgcggcggt gccgatccgg tggacgccga agccatccgt
     7981 ctctgggagc tgggctcggc ccaggcgggc ttcgcgctgc ccatctccta cttcggcggc
     8041 gtgtccgcgc tacggatggg cgagccccag cgggccacgg tctacctcca cgccgcggtc
     8101 gaaaacgcct tcccctggag tgacgagcgc tccgccgccc gcctcctcgg cctgatcgcg
     8161 gccgtccggg ccggcgccga gctctcctcg atccgcccgc ggctcacctc cgcgctggac
     8221 gaggcccggc aggtggcgac cctgcccggc cagcccaccg ctctcctggc gctctcgctg
     8281 gccggcctct ccgctctggc aggagatccc gcggagagga gccgctggga gtcgctggcg
     8341 cgcgaccgta ccgcgggcgc ccctccctgg gagcgcgcct tcctgcgcga gctgcccgat
     8401 ccgggcgctc tcacccccct cccctgaccc tcgtcaaaac ttcgagcggg ccgtccacat
     8461 cttctgacgc gccgctctcc agggccggac ccccgccggc gctggattcc cctctctccc
     8521 cccgcatcgc tcccgccgga ttccttgacc gcaggcctcc ccctcgcgtc cctcctgttt
     8581 cctgcaactt ctttgctgtc cgtcgtttag gtctccaatc atggcctggc agggaccttg
     8641 atctccaggg ccggcataac cgaggagggg ggtgacggcg agagaggacc gacgacgacg
     8701 agaccacacg aaagggaggg accggggccc gcgcggcccc ggcccgaatt cgcagccgca
     8761 gtccggaatc cgagcagtcc aacccaaacc cagcagtcca gaacccaaag gagcgatgtc
     8821 atgaatcgta cgtaccgttt cgccagcagc gtcaccctcg tcctgttttt ccttctcctg
     8881 tccgcgctcc ccggcaccgc cgccgagggc aagcgggcgg tcaacatcaa cagcgccgac
     8941 gcttcgcagc tcgcgctgct gccgcgcatc gggccgtcgg tggcacagcg gatcctcgag
     9001 ttccgcaagc agaacggccc cttcaagtcc gccgaggacc tgatgctggt gcggggcatc
     9061 ggcgagaaga ccttcgccct gatcaagccc tacgtggcgg tctcgggcga gacaacgctc
     9121 aaggagaagg tcaagggggc gaagccgtcc agctcgaagg agggcggcaa ggactccaag
     9181 gagggctccc ggtagcggct ccggggggcg ggccggccgg tccgcccccc ggccgttccc
     9241 ggagcgttcc tcatggggtc ccgcgagaag ggattcacgg tggtggaggc ctcgatctat
     9301 ggcgcggtcc tggcggcggc gatgctggtg accggtccgc ccctgctgca gctgtccggc
     9361 gacctgcggc tccgcgccgc ggcggcggag gcggcggcgg tgctccgcag cacgcggttc
     9421 ctggcggtcc gccacagcgc tcacgtggcg gtccgcttcg gagcctgggc gggcgggccg
     9481 atcacctatg cgatctaccg ggatggcgac ggcgacggcg tcctctcccg cgacattacc
     9541 cggggtgtcg atccggagct ctcgccgcca cgcccgctgg gggatggcca cgcgatccgc
     9601 ttcggcttcc cgccggggct gcgggtccgc gaccccggcg acccccgggt ctggatgaag
     9661 accgaggacc cggtccgctt caaccaatcc aatatggcct ccttcggtcc gctgggtgag
     9721 tccacgccgg gctcgctcta cctgaccgac ggcgaccgcc gtctggtggt ggtgcgtgtc
     9781 ttcgggcgca ccggcaagat ccgggtgctg agctacgact tcgacaccca gaagtggctg
     9841 cagagatgaa ccctccggcg cggggtggcc gccatccgcg tccgccccgc gccggacgca
     9901 ttgactcatt ccaaaggagt gcagtacctt ggcaccatga gaggcatagt cgttgtcacc
     9961 accgtcggca ccgaagaaca ggcttacctc atcgcgcgcg agctggtggg ccgccgccag
    10021 gcggcatgcg tgaatgtgat gcccggtgtg cgctcgatct atcgctggaa ggggaagatc
    10081 tgcaaggacg gcgagctgat gctgatcatc aagaccctcg agggtgagtt cgaaggcgtc
    10141 gaggcgacga tcaaggagct ccacagctac gagctgccgg agatcctctc cttcgaagtc
    10201 gcccagggcg agcggaactt cctggagtgg atccggagca gcgtcgacaa ggacgccgac
    10261 ttctccgacg aggaggagga cgagatcgcc tacgccgccg ccgatcccga cgacgaggcg
    10321 ttctagcctc ccccccgccg ctctccctct acgccggcac gccggccagc gcctggtcga
    10381 gatcggccag caggtcgtcg atgtcctcga tgccgatcga gaaccgcacc agcccatcag
    10441 agaggccgat cagcgcccgc cgctccgccg gcaccgaggc gtgggtcatc gacgccgggt
    10501 gcgaggccag gctctccacg ccgcccaggc tctcggcgag gctgatcacc cgcaggccgt
    10561 cgaggaacgc cttgcccgac tcatagctcc ccatctcgac gctgatcatg gcgccgaagc
    10621 ccgacgcctg ccgcttctgg atctcgtgcc ccgggaaatc gggcagcccc gggtagagga
    10681 cccgcttcac cttcggatgg ccgaccagga agtccaccac ccggcgggcg ttctgctcgt
    10741 ggcgctccat gcggacggcc agcgtcttga tgccgcgcat caccaggaac gaatcgaagg
    10801 gggagaggat cgccccctcc gacttctgga cgaaggcgaa ccactccgag tgctcggggc
    10861 gcgcggcgac cagcgcgccg ccgatcgagt cgctgtggcc gttgaggaac ttggtcgtgg
    10921 agtggaggac gaagtcgcag cccagctcca gcgggcgctg caggtaggga gagaggaagg
    10981 tgttgtccac cgccacaagg aggttgcgcg agtggccgag gtcggccgcc gcccgcagat
    11041 cggtgatctc catcgtcggg ttggtgggcg tctcgatgta cagcatcttc gtctcgggcc
    11101 ggatcgccgc ctcgacggcg cggaggtcgc tcgtgtccac ccaggagaag gtgacgccgt
    11161 aaggctccag cagccgggtg aagtagcggt aggtgccgcc gtagacgttc tgcgacacca
    11221 ccacgtggtc gcccgtccgc accagggtca tcaggcaggc gatggccgac atgccggagg
    11281 cgaaggcgtg gccggagacc cccttctcca gggaggccac gttctcctcg agcgcggcgc
    11341 gggtggggtt ctggacgcgg gcgtactcgt agaacgcccc ctcgcccacg ccctcctgga
    11401 cgtaggtgga ggtctggtag atggggaccg cgaccgcacc ggtccgtgga tcgggggact
    11461 ggccggcgtg gatggcgcgg gtggaaaagc ccaggttctt gggatcgcct ttcatgggaa
    11521 tcctcgggtc gtgtcggttc gaaggggagc gcccggagta cgggccgccg gatcggggcg
    11581 cattctagca aggcgggcta agatacgcgg ccatgagcga gccacggacc gattcgacga
    11641 cgccggccgg acctgaacag gtgttctccc ccatgcgctg ggaggacggc cggctcctcc
    11701 tgctggacca gacgcgcctc cccaccgagg aggcctggct ggagtgcgag acgccggagc
    11761 aggtggcgga cgccatctat cgcctggcgg tgcggggcgc gccggccatc ggcgtcgccg
    11821 ccgccttcgg gctggtcctg gggctgcagg ccgtgcccgc gccggagggc cgggaggcgc
    11881 tggcccggcg cttcgaggag gtctcccggc tcctcggctc cacccggccc acggcggtca
    11941 acctgcgctg ggcgctggag cgcgggcggg aggtgttcga ggcgaacatg gccagcgggc
    12001 cggaggcggc gacccgggcg ctcctcgcct gggcccacga gctccatgcc gaggatgtgc
    12061 gggtcaaccg ccgcatcggc gaccacgggg cggcgctgtt cggctcgggg gcgcgggtcc
    12121 tcacccactg caacacggga tcgctggcga cggccggcta cggcacggcg ctcggcatca
    12181 tccagtccgc ctggcgcgac ggcaagctcg gcatggtgtg ggtggacgag acccggccgc
    12241 tgctccaggg agcgcgcctg accgcctggg agctcaagaa gctggggatc cccttcaggc
    12301 tggtgaccga ctcgagcgcc ggcacgctca tggcgcaggg catggtggac cggatcgtgg
    12361 tgggggcgga ccggatcgcc gccaacggcg acacggccaa caagatcggc acctacacgg
    12421 tggccgtcct ggcccaccgg cacggcgtcc ccttctacgt ggcggcgccc ctgtcgacca
    12481 tcgaccgcgc gacggccacg gggcgcgaaa tccccatcga ggaacgcaag tcgggggagg
    12541 tgacggaggt cttcggcacc cgcatcgcgc ccgacgagac gccggcgctc aacttcgcct
    12601 tcgacgtcac gcccagcgag ctgatcaccg ccatcgtcac cgaagtggga gtgctggagc
    12661 cgccctacac ggagagcatt gccgcggcat ttggaacggc ggtcggccgc gccaagtcct
    12721 gaggtctgaa gaagctttgt cctttggctg ggtgggaagt tggtggaggc ggtgggagtc
    12781 gaacccacgt ccgagaaacg cgtctcgaga gcttctacgt gcgtatcctt ctatttagtt
    12841 gtcgccaacg gcgttttgga agaaggcacc tggcgccgag gccttcctcg taaaaatcca
    12901 ggtacgggta cgaggacccc gaacccttcg atccgcattg agtgtcgccc caagctggcg
    12961 ccgcggatca ggctccagcc gggacgtcac gaccgttttt taggccgcga gggcgaagtt
    13021 gtcgttcgca gttacaaggt accgttttta cgagcggaaa ctcgacacgc aactctcgct
    13081 ccgctctgtt ccccgtcgaa accggtacgc ccccagattg ccaaagaacc ttgctgctga
    13141 ctacagcacc agtataaacc acccgcgggt gggagagatt cccggcgcgg tcaggtcttg
    13201 ccgcgggcgg cttcttcctc tcccaggagg ccggcggcca tctccaggaa ctcgtcgggg
    13261 ccgctggtgc gcagacgggt cagcaccagg aaccagccgc cgagggcgag gaggacgccg
    13321 aagggggcca ggggcaggag gcgcgggtag aacggccaga gcacggtgag gaccgcgccg
    13381 gcgactgaga tcagcaggat caccagcgag gggatgtgga cgtagaggtt ctggtcgtcg
    13441 ggacggaaga cgacgcgcga gccctctgtc gtgggctcga cggcgagcgg gccggcgacg
    13501 aagccgcggc gcaggccggc gaggaccgga atgtggagcc gcccgccgcc accctggcgc
    13561 tcgagctcgc cgccccacat ctccgccgcc cgcgccaccg ccgagagcgt cgcctccggc
    13621 gggtccggca actcgatcac gtgctcggcc atgaaggggg cgggctaggc ggccttctct
    13681 tccccggtgt cgtcgaagaa gctctcgacg gcggcgagga aggaggggac gtcggggatc
    13741 tggttgatct cctgccggag acggcggccg ttgggcaggc cgtgggtgta ccagccggtg
    13801 aatttgcgca gcttgtggag ggcaaaggtg gagtgctcgc gctccgccac catccggaag
    13861 tggctcagga tcaggtcgcg ccggtcctcc agggtgggct cggagaagcc gctccccgac
    13921 atccgcgccg cgatctgccg gaacagccaa gggttgcgcg tcgcgccgcg ccctaccatc
    13981 acgccgtcgc agccggtctc ggccatcatg cgcagggcgt cctcgggctc gcggacgtcg
    14041 ccgttgccga tcaccgggat ggacagcgcc tccttgagcc gcgccaggtg cgaccagtcg
    14101 gcctcgccgg tgaacatctg gcgcgccgtc cgggcgtgca tggccacggc ggcgacgccg
    14161 ttcgcctcgc agagccggcc cagctccagg tagttgcgcc ggcagtcgtc caggcccagc
    14221 cggaacttga cggtcagcgg gatggtgagg acccccttca ccgcgcggac gatctgctcc
    14281 gcgagctcca ggtcccccat cagcgccgcc ccggcgcacc ccttgagcac cttgttggcc
    14341 gggcagccca tgttgatgtc gcagatgtcc gcgcccagct cctcgaccac cagcgccgcc
    14401 tcgcgcatgg tggcgacgtc cgagccgtag atctggatcg ccagcgggcg ctcctcctcg
    14461 gcgaagtaca tcagctcccg ggtccgcctg ttgcccgaga cgatggcccg cgaggagatg
    14521 aactccatcg acaccacgcc cacgccgccg atccgccgga cgatcagacg gaaatcgcgg
    14581 tcggtcaccc cggccattgg ggcgagccag agaggcggat cgacctggac gttacccact
    14641 tgcaccatgg cgacagattg taacgcgatg gagggcgccg gccttcccgg ccgccctcag
    14701 gcccggtgcc gggccgagcg gccgaggagc agggtgaggc cggcggcgat ggcggcccac
    14761 tgcaggccgc cggcgatcag gctccaggtg aggagcgtgg ggccgtccag gtgcaggtag
    14821 agcagggaga cggggaggtc catccaggtc agcacgttgc cgcggccgaa gacgccccag
    14881 gtctgccagg cccagagggc ggcggcggtg tggagcacgg cgccgacgat catcgccacc
    14941 agcgcggcgc ggggggaggg gcggagcctc acgcggccct cccggctccg gacggcgcat
    15001 ccgccgccag acggaacccg tagtcgctgt agcgcagatg gggagggatg gccgagcggg
    15061 cggcgcagcg gctcgccttg atctcgtgcc gccacgagcc cccacgggag gagcggcggg
    15121 tgccggcggc cggaccgcgc gggtcctccg ccggcgacac ggcgtagtag ccggcgtcgt
    15181 accagtccga gcaccactcg tggacgttct cgcccaggcc gaggaagccc agcgggtgag
    15241 gggaggggta ggcgtccacc ggctccggcc ccctggccca gcggcggtcg tagtcgggga
    15301 gcgactccgg cggctcgtcc ccccagggat agaggacgtc ccgcccggcg cgcgcggcga
    15361 gctcccactc ggcctcgctg ggcaggcgcg gacggtctcc ggagtcctcg agctcgccgg
    15421 ccagccaggc gcagtaggcc gcagcgtcct cccacgacgg gccgaccgcc ggcatccgcg
    15481 ggtgggaaaa ggccgcctcg ctccagaacg gcggcgccgg gtggccggtg gcgtccagga
    15541 agcgctggta ctcctcccgg gtcaccggcg cgcgggccag cctgaaggcc ggcacccaga
    15601 cctcgtgccg cgggttctcg ttgtcccgcg gcccgccccc catccagaag cgcccctcgg
    15661 cgacgccgat ccagtccatc tgcatgagcg atagcatacg agagttccct gccgccccgc
    15721 agtggctcgg cgctgtggct cgaaccggcg caggcgactg gcagcgagcg cggggggagg
    15781 gtggagcgca cacgaaggag ccgagcaggg gtcgagccac tgcgccgagc cactccgacc
    15841 ctatatgatc cccctccatg gcctggtaca aagagtggtt cggcgaggac tacctggatc
    15901 tctactccca ccgggacgag tccgaggccg aggcccacat ggacttcgtc gagcgccatc
    15961 tggacacggg gcaccggccg cgcgccgtgc tggacctcgc ctgcggtgcc gggcgccaca
    16021 ccgcggccct gcgccgccgc ggctaccgga cgctcggggt cgacctctcg ctgaccctgc
    16081 tcgcccacat gcaccggctc ccccgggtgg ccggcgacat gcggtgtctg cccttcaccg
    16141 ccggcagctt cgactgggtc ctcaacttct tcacctcctt cggctacttc gagcgcgagc
    16201 gcgagaactt ccaggtgctg gaggagatcg tgcgcctgct cacgcccggc ggccggtttc
    16261 tcatcgatct gctgaacccc ggctacgtcc tcgcccgcct cgagccccgc gagacccagg
    16321 agcacgacgg ccggcgcgtc gagatcgagc gctggtacga cccggccaag ctccgcatca
    16381 acaagcgcat ccgggtcctc gagccggggc gcccggcccg cagcttcgtc gagagcgtgc
    16441 gcgcctaccg ggcggaggag gtcaccatcg gcctgcgctg ggccgggctc gaggtctcca
    16501 acctcttcgg cggcttccag ggggagcctt atcagaatga cagcgaacgc ctcatcatcg
    16561 tcggctacaa gcccgcctga gacgccgcga gggggggagc cggtcgcgac agcgctgccg
    16621 ccgctgccct cggcctggcg cgccggccgc gacctcgacc tcctggcccc cctgcgcctg
    16681 ctgaccgggg cggagccgcc gtccggtccg gcgccggtgc tcgccggcgc cgcggccgtg
    16741 gctcgccgcg agctggcgga ggccctggcg gtcagcaacc gcgcctatgg ccacgagggg
    16801 gcggacgccc tggcccggcg tctcgccgac cccgccaccc gcgtcatcgt caccggccag
    16861 cagccgggcc tcctcggcgg gccgctctac accttcgcca aggcggtggc cgcggcgcgc
    16921 tgggcggcgg ccctggaggt ggccggccag cccgcggtcg ccgtcttctg ggtagcgacc
    16981 gaggaccacg actgggccga ggtctccgcc gccaccatcc tcgcctccgg cggtccccgc
    17041 accttcgacc tcggccccga tccggcgccg ctgacgccgg tgggtatgcg caccctgggc
    17101 gacggcgtca ccggcgtgct cgacgagatc atggccgccg tgcccaacga acgctacgcc
    17161 gagtggctgc gcaccgtcgc ccgctggtac cggccggacg cccgcttcgg cgaggccttc
    17221 tgccggctgc tcgcccacat gctgggggac cgctgcccgc tgctcctcga cgccatgcat
    17281 ccggcgctca aggcggcgca gcgcccgtgg ctgcggcggc tggtggagcg gcggcgcgag
    17341 gccgaggagg ccctggcggc ccgcgaagcg gagatcaccg gccggggcta ccccctccag
    17401 gtggcgccgc agcgcggggt gagccccctc ttcctccttc atcgcggcga gcggcggcgc
    17461 gtcgagtggc gtggcgaggc cggatatgcc ctgcgcggcc gggacgacgc cggcggcacc
    17521 gtcaccgacc tcctgcgcgt catcgacgag aaccccgggg tggtcacgcc cggcgtcctt
    17581 gcgcggccgg cgatccagga cgccgtcctc ggcagctgcc tccaggtgat gggcccgggc
    17641 gagctctcct acctggccca ggtggcggcg gtctatccgg tgctcgaggt agaggccccc
    17701 tggacgtcgc tgcggccgca gacgctggtc ctcgaatccc accaggcgga gaggctggcc
    17761 gaggcgggga tcaccctggc ggacctgctc ggcgaccgcc agcgtctgga ccgcgcgctc
    17821 gcctcctcag aggatggcga gccggtcgcc gcggtccggg cgcgggtgga gcaggcgctg
    17881 gacgacctca aggcgtcgag cctggcggcc gatcccaacc tggagcggcc gctggagaag
    17941 acccgcgagc agatcctgcg cgccctcgac ctcttcgccg agaagacgac cggggcgctg
    18001 gcgcgcaaga acgaggtgcg gagccgccgc gtcgagcagc tccgcgaagc ctgcctcccc
    18061 ctgggcaagc tccaggagcg ggtggtctgc gccgcccact tccagggcaa gcacggctcc
    18121 cgcttcgccg agagctactg ggagcagatg gggctcgacc ccgggacctt gcaagtgatc
    18181 tctccctagg gaggggaagg ggaaaggacg aggatgaagc tggacgttct cgcgatcggc
    18241 tcccacccgg acgacgtgga gctgggctgc ggcggcacgc tggccctgat ggcccggcag
    18301 gggcgcaggg tcggcatcct ccatctgacc ggcggcgagc ggagcacccg cggcacgccg
    18361 gccgagcggc gggccgaggc cgaggcagcg gcgcaggccc tgggggccgc ggagctggcc
    18421 ttcctcgact gcggcgacgc cagcttccgc tatggcgagg ccgaggaaga cgccctgatc
    18481 ggcatcctgc gcgtctggcg gcccgagctg gtgctcggcc ccacgccgcg cgaccgccac
    18541 ccggaccacg gccgcgccca tgagctggtg cacgccgcct gcttctactc cggtctggcg
    18601 agccggggag agcgggacgt gaaaccccac cggccggcgg ccgtcttctc ctatatgcag
    18661 cacgacctct tcgagcccag cttcatcgtc gacgtcaccg ccgtctggga gaccaagctc
    18721 caggccctgg ccgcctaccg cacccagctc taccagcgga ccgcagggga aagagagggc
    18781 aaggacaccg cgccggtcac caaggtgtcc acgcgcgagt tcgccctcgc caacgagggg
    18841 cggtcgcggc acttcggcct gctcatcggc gccgagttcg gcgagccgtt ctggagccgg
    18901 ctgccgctcg cggtggccga cccgatgacc atcctgccgg gaggtgtgcg gtgaagatcg
    18961 gcatcagctg ctatccgagc tatggcggct cggggatcgt ggccaccgag ctggcgatgg
    19021 cgctcgccga cggcggggac gacgtccaca tcatcagcta cgcgttcccg tcgcggctgt
    19081 cgatcctcaa cccgcggatc ttcttccacg aggtggtgac cccccactac ccgctgttcg
    19141 agtacccccc ctactccctg gctctggcca ccaaaatggt ggaggtgggg cgccaccacc
    19201 agctcgacct gctgcacgtg cactacgccg tgcccaacgc ggtgtcggcg atcctggcgc
    19261 gccagatcct ggcgccgcag cagctgccgg tggtcaccac cctccacggc accgacgtca
    19321 ccctggtggg caacgacccc aactacctgg agaccacgcg ctgggggatc gtcgagagcg
    19381 acggcgtcac cgcggtctcg gagtccttgc gccagaccac cgtcgagcag ctcggcatcc
    19441 acaagcgcat cgacgtcatc cccaacttca tcgacccggc gcgcttcgag tcggcgcggc
    19501 actcgccggg ggcgcggcgc tgggccaagt cgaacgagcg catcctcatc cacatctcga
    19561 atttccggcc ggtgaagcgg gtgctcgatg tggtcgagat cttcgaccgc gtccgccgcg
    19621 aggtcccctg ccgcctcctc atggtgggcg acggcccgga ccgcgccaag atcgagcagc
    19681 gttgccgcga gtgccggctg tgcgacgaca tcaccttcgt cggcagcctg ccgctcatcg
    19741 aggaggtgct ggtcggcggc gacctcttca ttctcccgtc ggagacggag tcgttcggcc
    19801 tggccgccct cgaggcgatg gcctgcgagg tgccggtgat caccacccag gccggcggcc
    19861 tgcccgaggt ggtggtcgaa ggggagaccg gccacctccg ccccgtcggc gacgtggacg
    19921 gcatggccga ggcggcggtg cgcctcctcc aggacgagcc gcggcggcgc gccatgggcg
    19981 aggcgggccg gcggcgggcg gtggagatct tcagccagga cgccgtcgtg gcgcgctacc
    20041 gtcagctcta tgaccgggtg atgcagcggt cctgagaagc gatgtctgcc tatcccgctc
    20101 ccggcggcga ggcccgcgcc gagatccgcg agaagggctc gcgcttcctg gcggtgctgg
    20161 ggccggcggc gggggaggag gaggccaagg cgcggctcgc ggacctggaa aagctctacc
    20221 ccgacgccac ccaccactgc tgggcctggc gtctcggctg gccgccgcgg gagcgcagct
    20281 ccgacgccgg cgagcccggg ggcacggcgg gggtgcccat cctccaggtg ctgcgcggcg
    20341 ccggcctctc cgacgtgatg gcggtggtgg tgcgctggtt cggcggcgtc aagctgggca
    20401 agggggggct cgcccgggcc tacgccggag cggcccggga ggcgctccac gagctgccgc
    20461 tg
//
LOCUS       DASPJI010000895        17404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_284019, whole genome shotgun sequence.
ACCESSION   DASPJI010000895 DASPJI010000000
VERSION     DASPJI010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 17404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17404)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17404
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284019"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..490)
                     /locus_tag="VEL74_25335"
     CDS             complement(128..490)
                     /locus_tag="VEL74_25335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65928.1"
                     /translation="MLQAGLTHEMTCTVTPDMSPPHLAGILSTSRMIGLMEDTCLAAV
                     QPLLAPGQTTVGTRVEVTHVGTAWAGEEVTIRVRLTKVTQRRLLGFEIEIAAPAGIIS
                     TGTHQRLVVDRSKFARPA"
     gene            complement(465..2258)
                     /locus_tag="VEL74_25340"
     CDS             complement(465..2258)
                     /locus_tag="VEL74_25340"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HYG65929.1"
                     /translation="MIFALSGALLLAASIACRSGMPPADTGNAAAASRRVILISLDGA
                     GASAFRELYRQGALDRGGFERFFREGQVADALIPVDPSLTATNHISLATGFPPAVTGI
                     VGNAFYSGGSISAQTVNAFEAPVGTQTLWEAARRQGRQVGVLTWPGADARDERRTASW
                     GLVYTNAADLEAQILTLSGADWKAVPGHSARSSAQVRIGEGEGTRTLDLVAVDTEDDG
                     ETRYDRVAVRAGQRETAPLRAGEWGRLAWDAGEGHTSSWLKLLALAPDLSEVRLYLGG
                     IHRTVAYPDTFAGSLAEHSIYWPGEPDNRALVAGWQGRAGIDLATWTEQAEHLAAFWG
                     ETLRFAAAREDWDLLLSYNPVIDLAGHRLLLLDARQAGFSPERRDELTRARHRVWQAV
                     DAELRKLLAGLDMTRTTVVVVSDHGMVPVHTSIDPDEVLAGLGGTTGRPAVSAIANGG
                     AAFIYVEAGADPAIRASLLSDLASRYAEWRVDGEAPIEKVLTHEEAARIGLGHPNSGD
                     LVLFARPGYTFRALPPGTAAAPAPVYGAHGHLTTHPEMQALYMAIGAGIAPGRGGTVR
                     ATEVAPRVAAWLGIEPPGRNPSGNAASRPDP"
     gene            complement(2624..6259)
                     /locus_tag="VEL74_25345"
     CDS             complement(2624..6259)
                     /locus_tag="VEL74_25345"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HYG65930.1"
                     /translation="MSGFLNESDIERSLLEQLAELGYDTATGAEISPGGPREERRSYG
                     DVVLRERLRTALVRINAHLPAGAVEEALRRVERSEVPGLVASNRRFHLMLADGVKVEV
                     PRPARAGGGFSYETVTLVDFERPEENDYLAVSQLRVVEGKRQRRLDVVVFVNGLPLAL
                     FEIKSPADENATVRDAFKQIQTYKTEFPTLFGFNEILVATDGMEARAGTLTADWDRYM
                     PWRTVEGRDVAPASLPQLDVLVAGMFGKTRLLDLLRGFVVFEDDGRGAVKKMAAYHQY
                     HAVRKAVDCTLRAKMGDRRIGVVWHTQGSGKSLSMVFYAARIAREREMRNPTLVVLTD
                     RNDLDDQLFGTFAACQDLIGQPPEKAGSRDELREILQSKASGGIVFTTIQKFLPDQKG
                     DRHPRLSERDNIVVIADEAHRSQYETLDGYARHMRDALPHASFIGFTGTPIDIGDKST
                     TALFGDYIDTYDIERAIQDGATVPIYYEARLAKIALKDEEMPRIDPDFEEVTEAEEAG
                     DKERLKSKWSQLEKLVGAPERVARVAADLVEHFENREAAQRGKGMIVAMSRRICVELY
                     KEIVRLRPEWHDPDDEKGEIKIVMTGSASDEAEWQPHIRPKAGRERIAKRFRDADDPL
                     KLVIVRDMWLTGFDVPSLHTMYVDKPMHGHTLMQAIARVNRVFSNKPGGLVVDYLGLA
                     DQLRQAVRTYTQSGGQGRATVDIEEAETALRKSYEVVRGIMHGFDVEGAVHGTATQRS
                     RAMAAAIDFVFELGPEGQKRYLDAVTDLSKAFALAGALDYAAQILDEVAFFQSVRAAI
                     VKRSPAEGGRRSREDVEASIRQIVSRSIVSDEVVDIFSAACLEKPELSILSDSFLEEV
                     RQLPYRNLAVELLRRLLTGEIQRREKKNLVQGHSFREKLEEAIRRYQNRSIETVQMIL
                     ELIEMAKQLREADRRGEELGLTEEERAFYDALETNDSAVAVLGDETLRAIARELTEIV
                     RRNATIDWTERESTRANLRRRVRRLLNQKGYPPDKQEKATDTVLAQAEALGDVFAEEA
                     QAAPPPPPERPFRIVPPAEVVPFENAVPVLELAVAAGRFSGEQEVDGFQDAAAHPDRF
                     EWAELPDYYRPRPGMFVARIVGESMNRRIPNGSWGLFRIHPHGSREGKVLLVQHREIE
                     DTDLGGHFTVKRYHSEKVQHPDGTWEHTRILLSPDTHAEGYEPIVIESADAPELRVIA
                     EWLGLVG"
     gene            complement(6583..7770)
                     /locus_tag="VEL74_25350"
     CDS             complement(6583..7770)
                     /locus_tag="VEL74_25350"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HYG65931.1"
                     /translation="MEARLPLDWRILSLEACVEALIDYRGKSPIKSSHGVPLVTAKIV
                     KSGRILPPEEFIPLADYDLWMRRGLPRAGDVVVTTEAPLGEVAQLGPEKVALAQRIIL
                     LRGRKNLLDQDYLLYAMQSSIVQRQLQARATGTTVHGIRQSELRKISLPVPPLVEQKA
                     IAYILGALSQKVHLNEDTNRTLEAMARAIFKSWFSDGESGSQNIEEIYRVANVTYGAP
                     FSSSLFNEKGEGEPLIRIRDLEAQSPSLFTTETPARARLAQPGDILVGMDGEFKAHIW
                     CGPPAWVNQRVCLVEPKPHIPRAFLLYSLEEPLSYFERAKTGTNVIHLGKADIDTFRL
                     RLPPQKILQTFGELTEPMLDKIILNRQESRTLAALRDTLLPKLLSGEIRIKQAEKMVG
                     EAV"
     gene            complement(7760..9358)
                     /locus_tag="VEL74_25355"
                     /pseudo
     CDS             complement(7760..9358)
                     /locus_tag="VEL74_25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
     gene            complement(9464..9577)
                     /locus_tag="VEL74_25360"
                     /pseudo
     CDS             complement(9464..9577)
                     /locus_tag="VEL74_25360"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(9674..9955)
                     /locus_tag="VEL74_25365"
     CDS             complement(9674..9955)
                     /locus_tag="VEL74_25365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65932.1"
                     /translation="MNTILRGMAFIPEETVSISPETSSISGETDPISPETSVLSEGTA
                     SLSLETAFIPEGTFGISPGMANLSRETSIASEGMTMIHEAHEMALPARC"
     gene            complement(10091..10576)
                     /locus_tag="VEL74_25370"
     CDS             complement(10091..10576)
                     /locus_tag="VEL74_25370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65933.1"
                     /translation="MSEVPPTPNAFTAGFLARLGERDEPPTAGEAAVAGPWKIEEIPG
                     RGFGLFRAGQGLARGGRPAVIASARWLALVFAAVLPGTGRDPLLRLGKEADAGGLYPV
                     TLDTGEAVAGLDVFDDTLVEHANALIHLLRSPLSLADALEAGGAESLTCCGAILDERA
                     G"
     gene            11013..11429
                     /locus_tag="VEL74_25375"
     CDS             11013..11429
                     /locus_tag="VEL74_25375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65934.1"
                     /translation="MPNVTRSHAAVRKALEQYNATLEANAAELPQLDGTRTSIQNVLQ
                     TLRESSVEQDFFRAQKQQATKRQEAAVDEGRKLISYVKVVLRQHYGSRSEKLAEFGVQ
                     PFRGRARTAVAEVKPRNPGATVAPEVQEPKAEEREG"
     gene            11532..11756
                     /locus_tag="VEL74_25380"
     CDS             11532..11756
                     /locus_tag="VEL74_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017294222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HYG65935.1"
                     /translation="MSTTALRVELPMTVTEDEARLLLAVKTFEVGKASLGQAARIAGL
                     SKRAFMELLGRYGVAVFNYSPEELEGEVES"
     gene            11753..12202
                     /locus_tag="VEL74_25385"
     CDS             11753..12202
                     /locus_tag="VEL74_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012801509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HYG65936.1"
                     /translation="MREAVVADSACLIALERIGRLDLLQSLFDSVFIPPEVRREFGTD
                     LPWLRIEAPADRKLVTTLQMLVDSGEAEAIALASDMDVPIILDDLKARTVARGLGLSI
                     IGTVAILVRAKRAGFISAVNPLIGELEGAGFRLGAALKREVLRLAGK"
     gene            12312..>17404
                     /locus_tag="VEL74_25390"
     CDS             12312..>17404
                     /locus_tag="VEL74_25390"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG65937.1"
                     /translation="MTTAQLLSRLRDLEVRLWAEEDRLCFHAPKGAITPEIREALGRH
                     KPEVLALLRGGRSLAEGAGPEPGPAPESGPASLSFAQRRIWFLTQLEPESAAYNITRA
                     ARLEGRVRVEVLERSLAEVVRRHAILRTSFTSREGEPSQIAHPPAGLALPVIDLQALP
                     AEARRVEADRLAAEEARRPFDLRRPPLLRVFALRLAAEEHVLPTSLHHIAGDAWSTAI
                     LFRELGALYEAFSRGLPSPLPEPPLQYTDYARWQRGRLAGALLEEQLAYWTEQLADAP
                     PSLELPADRPRSAAASFRGTAHLSLVPPATVARLQAFTRLQSGEEGGTTPFMVLAAAF
                     ATLLARWSGQDDLVVGLPVANRGHTSLEGLIGLFLDTVALRVRLGGDPTFSELLGRTR
                     QATLGAYAHQDLPFERLVEALRPERQLHQTPIFQVVLSLQNVPRSDLAASSFRLSLLP
                     PTNPSAQFDLVCDMEEAADGLRISWRYRTDLFDATTIARLGEQYQLLLESALEDPGRP
                     IAELPLLDPARRHQMLVEWNGAGVPATEPVCLHERFAEQAAARPDSVAVSFEDLSLTY
                     GELALRARRLAGRLRALGVGPDVRVGVRLERPAEMVVSLLGVLEAGGAYVPLDPGYPA
                     ERLDFMARDADLRVVIDQAWIAPDGGDPSKPEATPPDALAYVIYTSGSTGRPKGVGIS
                     HAAADRLFTATVPWFGFGPGDVWTLFHSFAFDFSVWEMWGALLHGGRVVVVPERVRRS
                     PEELLDLLVREGVTVLDQTPSSFRQLMAVEGEGPALPLALRWVIFGGEALDFQSLAPW
                     MARHGDSRPRLVNMYGITETTVHVTYREVTLGDLPEIPRGGGASPPGRGVVGSGGGQE
                     GGFPGSAAGEAAGASVKTAPLLTSPAPTHAPAQGGRHHHPLRPELNPSLLGVAIPDLQ
                     VYLADRGSAPVPIGVPGEVWVGGAGLARGYLGRPDLTADRFRPHPWSETPGARTYRSG
                     DLARRRPDGDLEYLGRIDHQVKIRGYRVELGEVEAALTAFPGVRQAAAVVSEARLLGY
                     VVGEPGVPLDLAALRAFLEGRLPGPFVPSALVPLAALPITPGGKLDRAALAQAGPVPE
                     PAAPISAPAAAGPVAEALAGIWAEVLGVKAVGPGDDFFRLGGHSLLATAVLSRVRRAF
                     RVELPVAALFEAPTLAGFAARIESEMRGGESFEGPPLVARPRYGGAPLSYSQRRLWFL
                     DRLAPGNPFYNVPVVLRLRGGLRPAALEAALAEIVRRHEILRSALPELDGEPVQAAAP
                     ALDVRLPLVDVSGLPEEIREGEADRLADEEGRRPFDLAAGPLFRFSLLRTEPGDHRLL
                     LTLHHVVTDAGSMGVLRRELETFYEGGASLPDLPLQYADFALWQRDWIAAGGMEGQLA
                     WWKERLAGAPEEPGLPFDRPRPARERFRGGAVPLRLPVATAAAVRAAARREGMTSFMV
                     LLGAFQALLARLTGEEDVVVGTPVANRARPEIEGLIGFFSNTLVLRTDLSADPEGVEL
                     LDRARQTALAAYAHQDLPFEVLVEALAPERHLARNPLFQVLLVYQRLEGKGGLSDLAG
                     LLVDGAPPQGAAGTGSAKFDLTLALVEGEETFEGAVEYDADLFDPPTAARIAGQLTTL
                     LAELAGDSPPRLGDLPLLLAPERHQLAVEWNDVRDEMGLQAAGSPVHAGFEAWAGREP
                     AAPAVVWNDSTLTYGELERLSAALASRLR"
BASE COUNT         2529 a         5831 c         6068 g         2976 t
ORIGIN      
        1 ctgccgcgct acgcggtgcc cgacgaggtg gtcctccgcg agaccctgcc ggtgctgccg
       61 agcggcaagg tcgaccgccg cgccatccgc gatctggtcc tggaataggg agccgagcag
      121 gctcgcgcta ggcgggccgc gcgaacttcg accggtcgac gacgaggcgc tgatgagtgc
      181 ccgtgctgat gatgccggcg ggggccgcga tctcgatctc gaagccgagc agccgtcgtt
      241 gcgtgacctt cgtcagccgc acgcggatcg tcacctcttc gcccgcccag gcggtgccga
      301 cgtgggtcac ttcgacgcgc gtgccgacgg tggtctgacc cggtgccagc agcggctgga
      361 ccgccgcgag gcaggtgtct tccatcaagc cgatcatccg ggaggtcgag aggatccccg
      421 ccaggtgcgg ggggctcatg tccggcgtga ccgtgcaggt catctcatgg gtcaggccgg
      481 cttgcagcat ttccggaagg gttccgcccg ggaggctcga tgcccagcca ggctgccacg
      541 cggggcgcga cctccgtcgc gcggaccgtc cctccccggc ccggcgcgat gccggcgccg
      601 atcgccatgt agagggcctg catctcgggg tgggtggtga gatggccatg cgcgccgtag
      661 accggtgccg gggctgcggc cgtgccggga ggcagcgcgc gaaaggtata gcccgggcgt
      721 gcgaagagga cgaggtcgcc gctgttggga tgaccgaggc cgatccgagc cgcctcctcg
      781 tgggtgagga ccttttcgat cggcgcctct ccgtccaccc gccactccgc gtagcggctg
      841 gcgaggtcgg agagcagaga cgcgcggatt gccgggtctg cgccagcctc gacatagatg
      901 aaggccgcgc cgccgttggc gatggccgag accgccggcc tgccggtcgt gccgccgagc
      961 ccggcgagca cctcatcggg atcgatggac gtgtggaccg gcaccatgcc gtgatcgctc
     1021 acgaccacta ccgtggtccg ggtcatgtcg agcccggcca acagcttccg gagctcggcg
     1081 tcgaccgcct gccatacacg gtggcgcgcc cgcgtgagct cgtcgcgccg ctcgggagag
     1141 aaacctgcct ggcgcgcgtc gagcaacagg agccggtgtc cggccaggtc gatgacgggg
     1201 ttgtaggaca ggaggaggtc ccagtcctcg cgggccgccg cgaaccgcag cgtctcgccc
     1261 cagaacgccg ccaggtgctc ggcctgctcg gtccaggtgg cgagatcgat acccgccctg
     1321 ccttgccagc cggcgacgag cgcgcggttg tcgggctccc cgggccagta gatcgagtgt
     1381 tcggcgaggg agccggcgaa ggtgtccgga taggcgaccg tacgatggat gccgccgagg
     1441 taaagacgga cctccgaaag atccggggcg agcgcgagga gcttgagcca cgaggaggtg
     1501 tgcccctctc cggcatccca ggccaggcgt ccccattcgc cggcccgcag cggcgccgtc
     1561 tcgcgctgcc cggccctcac ggccacacgg tcgtagcggg tctcgccatc gtcttcggtg
     1621 tccacggcga cgaggtcgag cgtccgggtg ccctctcctt ccccgatcct cacctgagcc
     1681 gacgaccgag cggaatggcc cggaacggcc ttccagtcgg cgccggagag ggtgaggatc
     1741 tgcgcttcga ggtcggcggc gttggtgtag accagccccc acgaggcggt acggcgctcg
     1801 tcccgcgcgt cggcgccggg ccaagtcaac actcccacct ggcgcccctg gcgccgggca
     1861 gcctcccata gggtctgcgt gccgacaggg gcctcaaagg cgttcaccgt ctgcgcggag
     1921 atcgagcctc cggaatagaa cgcgtttccc acgatccccg tcaccgcagg gggaaagccg
     1981 gtggccagac tgatgtggtt ggtcgcggtc agggacgggt cgaccgggat cagcgcgtcc
     2041 gccacctggc cctcgcggaa gaaacgttcg aacccgcccc ggtcgagcgc gccctggcgg
     2101 tacagctcac ggaaggcgct ggcgccggcc ccgtcgaggc tgatcaggat gacgcgccgg
     2161 gacgcggcgg cggcattccc tgtgtccgcc ggcggcatgc ccgaacggca ggcgatgctc
     2221 gccgcgagca ggagcgcccc cgacagggcg aagatcattc gtctctcgag cgccatgacg
     2281 cggccacgag tctagtcctc gccccgggtg ttgtcgaggt gcccgccccc tgacgccgtc
     2341 tcggagcccc ggggacttcg ttacgaagtc cacagccttt cagtgagcgt ccaaggacgg
     2401 agagccgaag tcccgggacc gcggctcgag gtctcgggac ctggtcacga agtccgcggc
     2461 ctttgagcgc gggtcacggg accgcgcgcc gaggtcccgg gacatcatca tgaggtcccg
     2521 ggacctggtc ctgaggtcca cggtctttta gtgcgggttc aagggcggag agccgaagtc
     2581 ccgggacttg gagaggaagt cccgggactt cgagacggcg tccctagccg acgaggccca
     2641 gccactcggc gatgacccgc agctcggggg cgtcggcgct ctcgatgacg atcggctcgt
     2701 agccttcggc gtgggtgtcg ggcgagagga ggatgcgggt gtgctcccag gtgccgtcgg
     2761 ggtgctggac cttctcgctg tggtagcgct tgaccgtgaa gtggccgccg aggtcggtgt
     2821 cctcgatctc ccggtgctgg acgaggagga ccttgccctc gcggctgccg tgaggatgga
     2881 tccggaagag cccccaggag ccgttgggga tgcgccggtt catggactca ccgacgatcc
     2941 gggccacgaa catcccgggc cgaggccgat agtagtccgg cagctcggcc cactcgaagc
     3001 ggtccgggtg cgccgcggcg tcctggaagc cgtccacctc ctgctcgccg ctgaaccgtc
     3061 ccgccgccac ggccagctcc agcaccggca cggcgttctc gaacggcacc acctccgccg
     3121 gcggcacgat gcggaacggc cgctccggcg ggggaggggg cgccgcctgc gcctcctcgg
     3181 cgaatacatc ccccagagct tcggcctgag cgagcacggt gtcggtggcc ttctcctgct
     3241 tgtccggggg atagcccttc tgattgagca gccgccggac gcggcggcgg aggttggcgc
     3301 gtgtgctctc gcgctcggtc cagtcgatgg tcgcgttcct gcggacgatc tccgtcagct
     3361 cccgcgcgat ggcccgcagg gtctcgtcgc cgagcacggc cacggcgctg tcgttggtct
     3421 ccagggcgtc gtagaaggcc ctctcctcct cggtgagccc cagctcctcg ccccgccggt
     3481 ccgcctcccg gagctgtttg gccatctcga tcagctcgag gatcatctgc accgtctcga
     3541 tggaccggtt ctggtagcgc cggatggcct cctccagctt ctcccggaag gagtggccct
     3601 ggacaaggtt cttcttctct cttcgctgga tctcaccggt gaggagccgc cggaggagct
     3661 cgaccgccag gttgcggtag gggagctgcc gcacctcctc caggaagctg tccgagagga
     3721 tggagagctc cggcttctcc aggcaggcgg cggagaagat gtccaccacc tcgtccgaga
     3781 cgatcgaacg ggagacgatc tgccggatcg aggcctccac gtcctcgcgt gaccgccgtc
     3841 ccccctccgc ggggcttcgc ttgacgatcg cggcgcgcac cgactggaag aaggccacct
     3901 cgtccaggat ctgcgcggca tagtcgaggg cgccggccag cgcgaaggct ttggagagat
     3961 ctgtcactgc gtccaggtag cgcttctggc cctccgggcc cagctcaaag acgaagtcga
     4021 tggctgcggc catggcccgc gagcgctgcg tggccgtgcc gtggaccgct ccttcgacgt
     4081 cgaaaccgtg catgatgccc cgcaccacct cgtacgactt gcggagcgcc gtctccgcct
     4141 cctcgatgtc gaccgtggct cgcccctggc cgccgctctg ggtgtaggtg cgcaccgcct
     4201 gccggagctg gtccgcgagc cccaggtagt ccaccaccag gccgcccggc ttgttgctga
     4261 acacccggtt cacccgcgcg atggcctgca tcagcgtgtg gccgtgcatc ggcttgtcca
     4321 cgtacatggt gtggagggag gggacgtcga agccggtcag ccacatgtcg cggacgatga
     4381 ccagcttcaa gggatcgtcc gcgtcccgga agcgcttggc gatccgctcc cggccggcct
     4441 tggggcggat gtggggctgc cactccgcct cgtcggacgc cgagccggtc atcacgatct
     4501 tgatctcccc cttctcgtcg tccgggtcgt gccactccgg gcggaggcgt acgatctcct
     4561 tgtagagctc gacgcagatc cgccggctca tggcgacgat catccccttg cccctctggg
     4621 cggcctcccg gttctcgaaa tgctctacca gatcggcagc cacgcgggcc acgcgctcgg
     4681 gggcgcccac cagcttctcg agttgggacc acttgctctt gaggcgctcc ttgtcgccgg
     4741 cctcctcggc ctcggtgacc tcttcgaaat cggggtcgat ccgcggcatc tcctcgtcct
     4801 tgagggcgat cttcgccagc cgcgcctcgt agtagatggg cacggtcgcg ccgtcctgga
     4861 tggcgcgctc gatgtcatag gtatcgatgt agtcgccgaa gagggcggtg gtgctcttgt
     4921 cgccgatgtc gatcggggtg ccggtgaagc cgatgaagct ggcgtggggg agcgcgtccc
     4981 gcatgtgccg ggcgtagccg tccagggtct cgtactggct gcggtgcgcc tcgtcggcga
     5041 tgaccacaat attgtcccgc tccgagagcc gggggtggcg gtcccccttc tggtcgggga
     5101 ggaacttctg gatggtggtg aagacgatgc cgccggaggc cttgctctgg aggatctccc
     5161 gcagctcgtc ccggctcccg gccttttccg gaggctggcc gatcaggtcc tggcaggccg
     5221 cgaacgtgcc gaagagctgg tcgtccaggt cgttgcggtc ggtgagcacc accagggtgg
     5281 ggttgcgcat ctcccgttcc cgggcgatcc tggcggcata gaagaccatg gagaggctct
     5341 tgccgctccc ctgggtgtgc cagaccacgc cgatgcgccg gtcccccatc ttcgcccgca
     5401 gggtgcagtc cacggccttc cgcacggcat ggtattgatg ataggcagcc atcttcttga
     5461 ccgcgccgcg gccatcgtcc tcgaagacca cgaagccgcg cagcaggtcg agaagccgcg
     5521 tcttgccgaa catcccggcc accaggacgt cgagctgcgg caggctggcc ggggcgacgt
     5581 cgcggccttc gacggtgcgc cagggcatgt agcggtccca gtcggcggtg agggtgccgg
     5641 cgcgggcctc catgccgtcg gtggccacga ggatttcgtt gaagccgaag agggtgggga
     5701 attcggtctt gtaggtctgg atctgtttga acgcgtcgcg gacggtggcg ttctcgtccg
     5761 cagggctctt gatctcgaac agggccaggg gcaggccgtt gacgaacacc accacgtcga
     5821 gccggcgctg cctctttccc tcgacgacgc ggagctgact caccgccaga tagtcgttct
     5881 cctccggccg ttcgaagtcc accagggtca cggtctcgta ggagaagccg ccgccggcgc
     5941 gggcggggcg gggcacctcc accttcacac cgtcggccag catgaggtgg aagcgccggt
     6001 tgctcgccac gaggcccggc acctccgagc gttctacccg gcgcagcgcc tcctcgaccg
     6061 ctccggcggg gaggtgggcg ttgatccgca ccagcgccgt ccgcagccgt tcgcggagca
     6121 cgacgtcccc gtagctccgc cgctcctccc gtgggccgcc gggggagatc tccgcgcccg
     6181 tcgccgtgtc gtagcccagc tccgcgagct gctccaggag agaccgttcg atgtcactct
     6241 cgttgagaaa tccgctcatg cgccgtccct ggtgattcga gaattcgaag cggatggtag
     6301 cacccgcgcc gtgagatttc agggcaagag cgggaagtga agactactta ccggatatga
     6361 ttacgaggga agcgagtcgg tctcgttcga ggatacctgt cacaaattct attcgccccg
     6421 aaggggcaca ggcataccgc ctggcgtttc aacgccagga agcgaagcgg cgcaggcagt
     6481 ttgtgtggaa ttgcctccgc cccttcaggg cttgggggag gttgggaggc cggtacctgg
     6541 cattgaaatg ccaggcggta tgcctgtgcc ccttcggggc gttcagaccg cttcgcccac
     6601 catcttctcc gcctgtttga tgcggatctc ccccgagagg agtttgggga ggagggtgtc
     6661 gcgtagggcg gcgagggtac gagactcctg cctattcagg atgattttgt caagcattgg
     6721 ctctgttagc tcaccaaacg tctgcaagat tttttgaggt gggagtcgaa gacggaatgt
     6781 atctatatct gctttaccta agtggatgac gttcgtcccg gtcttcgctc tctcgaagta
     6841 agaaagaggt tcttctaggc tgtaaagaag gaaggcgcga gggatgtgcg gcttcggctc
     6901 aaccaggcaa actcgctggt tcacccaggc cggaggtccg caccagatat gcgccttgaa
     6961 ctccccgtcc attcccacca ggatgtcccc tggttgtgca agtctcgccc ttgccggcgt
     7021 ctctgtggtg aacaaggaag gtgactgagc ctcaaggtcg cggatacgaa tgagaggttc
     7081 accttcccct ttttcgttga atagtgaaga ggagaatggg gccccgtacg taacgttggc
     7141 gactctgtag atttcttcga tgttctgaga ccctgactcg ccatcggaaa accacgactt
     7201 gaagatcgcc cgagccatgg cctccaaggt gcgattcgta tcctcgttca gatgtacctt
     7261 ttggctcagg gcgccaagga tgtaagctat agccttctgc tccaccagag gcggcacggg
     7321 aagcgagatc ttcctgagtt cactctggcg aatcccgtgc acggttgtgc ccgtcgccct
     7381 ggcttgcaat tgtctttgca ctatgcttga ctgcattgca tacaggagat agtcctggtc
     7441 taacaagttc ttccttcctc ttagcaatat tatgcgctgt gcgagtgcca ccttctctgg
     7501 gccaagctgt gcaacttctc caaggggggc ctcggttgtt actacgacat cgccggccct
     7561 gggaaggccg cggcgcatcc aaagatcata gtcggccaat gggataaact cttcgggagg
     7621 aaggattctc ccgctcttca ctatcttggc tgtgaccagg ggaactccgt gcgatgattt
     7681 tatgggcgat ttgcctcgat aatcgatcag cgcctcaacg caggcttcta gggatagaat
     7741 cctccagtct aggggtagcc tagcttccat atcccaggcc tctcagactt tcaagtatta
     7801 ttttctcgag tcgatttgac tcagagaaat tggccgtcag ctttagtgtt agccgcctca
     7861 tcgtttcgtc gaaaggttcg tcttcagggc catcttctac tgctccgaca tacctccccg
     7921 cagtcagcgc atgcccgtgc tcccggatct ccggcgtgga acctgccctg cagaaccccg
     7981 cccgatcctc gtacttgcca gcctcccgct cccctcgcca agcgtgatac gtccgcgcaa
     8041 tctccttgat ctcctcatcc ctaagatccc ggtgcgtccg gtcgatcatg tggcccatgt
     8101 tccgggcgtc gatgaacaac gtttctccct gccggttccg gaacctgtgg ttcttcttgt
     8161 cccgggcgag gaaccataga cagaccggaa tccccgtcgt atagaaaagc tgtcccggta
     8221 gagcgacgat gcagtccacc aggtccgcct cgacaaggtt cttccggatc tcaccttctc
     8281 ctgactgatt cgaggagagc gacccattgg acatgacgaa gccggcgatc ccgctgggag
     8341 agaggtggtg aagaaagtgc tggatccagg cgtagttggc attgcctacg ggaggcgtgc
     8401 cgtatttcca gcgcttatcc tcccgcacgc gctcgccgcc ccaatcggag tcgttgaagg
     8461 ggggattggc gagcacgtag tcggccttga ggtccgggtg gaggtcgcgg tggaagctgt
     8521 ccgcgggctc ggggcccaga ttggcgtcga tcccccggat ggcgaggttc atcttggcaa
     8581 ggcgccaagt ggtgtagttg gactcctggc cgtagatgga gaggtcggcg cggcggcctc
     8641 cgtgggcgac cacgaaacgc tcggaggaga cgaacatgcc ccccgagccg cagcagggat
     8701 cgaagacccg ccccttgagc ggctcgatca tctcgaccaa gagcttgacc actcactgcg
     8761 gcgtgtagaa ctcgcctccg ctcttccctt cggcggcggc gaagcggccc aggaagtact
     8821 cgtagacccg gccgaggaca tccttcgaac ggctggcgga gtctcccagg ccgatgccgg
     8881 agacgaggtt cacgacgccg ccgaggcgag ccttgtcgag ctggggacgg ttgtagtcct
     8941 tggggaggac ccccttcagg gaggggttct cccgctcgat ggccgtcatg gcgtcgtcga
     9001 tggccttgcc gatggtcgcg tgaggggcct cttcccggat gcgagaccag cgggcctccg
     9061 cggggaccca gaagatgctc tccgcggtgt actcgtcccg gtcctccggg ttggccccgg
     9121 gctcgaggag gatggcctgg cggcgctcct cgaaagcgtc cgagatgtac ttgaggaaga
     9181 tcaggccgag gacgacatcc ttgtactgag aggcgtccag gtggccacgc agcttgtcgg
     9241 cggcggccca gagcttgtcc tcgaagccga ggttggcgcc ggagccgttg ccggccgcct
     9301 tcgcggcccg gcgctccccg cgggcgccct gcttgcggcc agccggcgcc tcaggcagcg
     9361 gcgcgggagc cggctccgct gctggagcga gcggagtggg ggagcccggc tccccgaaga
     9421 gggcgcggag cttctccagg gcgatggggc tgggctccgc caccccccgc tcccactggg
     9481 agacgatggg ctgggtgacg ccggcctttt cggcgagctg cgcctgggac agcccggccg
     9541 cggcgcggcg gtctttgatc cactgcgagg tcttcatcga aggagtcagg gtttcggggc
     9601 tcaaacggtc ttcacggcag cccggagggg ctccgcggcg tacgtcgggg actgtcggtt
     9661 cacggggcga agtttagcac cgggcaggca gtgccatctc atgggcctca tgaatcatcg
     9721 tcattccctc ggaagcgatc gacgtttccc tcgaaaggtt cgccatcccc ggcgaaatgc
     9781 cgaacgttcc ttcaggaatg aaggccgttt cgagggaaag ggaggctgtt ccttcagaaa
     9841 ggacggacgt ttccggggaa atggggtccg tttcgccgga aatggaggac gtttcgggcg
     9901 aaatggagac cgtttcttca ggaatgaagg ccatcccccg aaggatggtg ttcattcccg
     9961 aaggagtgac ggccgttccc ggcggagcgg cgcgctttcc ggcggaatcg ctgccgaaat
    10021 tgtgcggaaa aggtggacgc ggccgtggcc tgccccatga gagagggaca ggccacggat
    10081 tccagcttgc ctaccccgcc cgctcgtcga ggatggcccc gcagcaggtg agggactccg
    10141 cgccacccgc ctccagggca tccgccagcg acagcggcga ccgcaggaga tgaatcaggg
    10201 cgttggcgtg ctcgaccagg gtgtcgtcga agacgtccag acccgctacg gcctcgccgg
    10261 tgtcgagggt gacggggtag aggccgccgg cgtcggcttc cttgccgagc cggaggaggg
    10321 gatcacgccc ggtgccgggg aggacggcgg cgaagacgag ggcgagccag cgcgcgctgg
    10381 cgatcaccgc cggccggccg ccgcgggcga gaccctggcc ggcccggaag aggccgaagc
    10441 ctctcccggg gatctcctcg atcttccacg gaccggccac cgcagcctcg ccggccgtgg
    10501 gcggctcgtc tctctcgccc agacgggcga gaaagccggc ggtgaaggcg ttgggggtag
    10561 gggggacttc ggacacactg ctaccatcaa gagaaccacg cataaggatc actgctcctt
    10621 gtgttcgtgg ccaggtccgg tcggtgagtg ccagctcccg tccgggccga ttcggcttca
    10681 atgccgggcc acgtgctgcc tggtgcgacg gttgaatccc tcgccgggaa gctttcgcgg
    10741 ggccgcgcgc cctccggcgt gtcctgctac gacctgatgc cccgattcta gaagagcacg
    10801 gcgagaaaat caagcggccc tgctgatttt ccctcttccc cagtcctgaa caaatgttgc
    10861 agaaatcccc tgtgcctaca ggggatcagg aagattttct cctgatcctg aacgaaatga
    10921 gcggctcatc cgtagacata tacaggcggg tcgctcgggc catggaggct ccgggaatgt
    10981 gcccgccggg accatccaga agaggagaag ccatgccgaa tgtcacccga agccacgccg
    11041 cggtccgcaa ggcgctggag cagtacaacg ccacgttgga ggccaacgcg gcggagctgc
    11101 ctcagctcga cgggacccgt acctccatcc agaacgtcct ccagaccctc cgggagtcca
    11161 gcgtcgagca ggacttcttc cgggcccaga agcagcaggc gacgaagcgc caggaggcgg
    11221 cggtggacga gggccggaag ctgatctcgt acgtcaaggt cgtcctccgg cagcactacg
    11281 ggagccggag tgagaagctc gccgagttcg gggtgcagcc gttccgcggc cgggcccgca
    11341 ccgccgtcgc cgaggtcaag ccgcggaacc cgggggccac ggtggctccg gaggtccagg
    11401 agcccaaggc ggaggagagg gaggggtgat acaggggtag tccagtgaag ggccggggcg
    11461 ggcgactgcc cgccccggcg gcgtcacttc ctgtaccatc gagccgtagc actcttcacc
    11521 aggaggcaga ggtgagtaca actgcgctaa gggtcgaatt gcccatgacg gtaaccgagg
    11581 acgaagcgag actcctcttg gcggtcaaga cgttcgaggt cggtaaggcc tccctgggcc
    11641 aggcagcgcg tattgctgga ctgtccaaaa gagctttcat ggagctcctg ggacgttacg
    11701 gtgtagccgt cttcaactac tcgcccgagg agttggaggg agaggtcgag tcttgagaga
    11761 ggcggtggtc gcggacagcg cctgtttgat cgctctggaa cgcatcggcc gtctcgacct
    11821 tcttcagtcc ttgttcgatt ctgttttcat ccctcccgaa gtgcggcgag agttcggaac
    11881 cgacttgccg tggttgagga tcgaagctcc tgccgatcgg aagctggtca cgacccttca
    11941 aatgctggtc gacagtggcg aggctgaagc cattgccttg gcctccgata tggacgtgcc
    12001 gatcattctc gacgatttaa aggcacgcac ggtggcgcgg gggctggggc tttcgatcat
    12061 tggcaccgta gcgatcctgg tgcgggccaa gcgggcaggg ttcatttccg cagtcaaccc
    12121 gctgatcggc gagttggaag gcgccgggtt tcggctcggc gcggcgttga agcgagaggt
    12181 tctgcgcctg gccggaaagt agggaggtac cccgccccgg tttgtccccc cttcctcttt
    12241 cccgcacacc accacacttg aaggaacccc tcgggttcct cctggattct cttctctggg
    12301 tggtcctttc gatgacgaca gctcagctcc tctcccggct ccgtgacctg gaggtccggt
    12361 tgtgggccga ggaggaccgg ctctgcttcc acgctcccaa gggggcgatc accccggaga
    12421 tccgggaggc gctcggccgc cacaagccgg aggtcctggc gctgctgcgc ggggggcgct
    12481 ccctcgcgga gggcgccggg cccgagccgg gaccggcgcc ggagagcggg ccggcttctc
    12541 tctccttcgc ccagcggcgg atctggttcc tcacccagct cgagccggag agcgccgcct
    12601 acaacatcac ccgcgccgcc cgcctggagg ggagggtgcg cgtcgaggtg ctggagcgca
    12661 gcctcgccga ggtcgtccgg cgccatgcca tcctgcggac ctcgttcacc tcccgcgagg
    12721 gggagccgtc ccagatcgcc cacccgccgg cgggcctggc gctgccggtc atcgacctgc
    12781 aggcgctgcc cgcggaggcc cggcgggtgg aggcggaccg cctcgcggcc gaggaggcgc
    12841 ggcggccgtt cgacctgagg cgcccgccgc tgctgcgggt ctttgcgttg cggctcgccg
    12901 cggaggagca tgtgctcccc acgtcgctcc atcacatcgc gggggacgcc tggtccacgg
    12961 cgatcctctt ccgcgagctg ggcgcgctct acgaggcgtt ctcgcgcggg ctgccgtcgc
    13021 cgctgcccga gccgccgctc cagtacaccg actacgcgcg ctggcagcgc ggccggctgg
    13081 cgggggcgct cctcgaggag cagctcgcct attggacgga gcagctcgcg gacgcgccgc
    13141 cgtcgctcga gctgccggcg gaccgcccgc gctccgccgc cgcttcgttc cggggcaccg
    13201 cgcacctgtc gctggtgcct cccgccacgg tcgcgcggct ccaggcgttc acccggttgc
    13261 agagcgggga ggaagggggg acgactccgt tcatggtgct ggccgcggcc ttcgccaccc
    13321 tgctcgcccg ctggtccggg caggacgacc tcgtcgtggg gctgccggtg gccaaccgcg
    13381 gccacacgtc gctggagggg ctcatcggcc tcttcctcga taccgtggcg ctgcgggtgc
    13441 ggctcggcgg ggaccccacg ttttccgagc tgctggggcg gacccggcaa gcgacgctcg
    13501 gtgcgtacgc ccatcaggac ctccccttcg agcgcctcgt cgaggcgttg cggcccgagc
    13561 ggcagctcca ccagacgccg attttccagg tcgtcctcag cctgcagaac gtgccgcggt
    13621 ccgatctggc ggcctcctcg ttccgcttga gcctgctgcc gcccaccaat ccctcggcgc
    13681 agttcgacct cgtctgcgac atggaggagg ccgccgatgg gctgcgcatc tcctggcgct
    13741 accggaccga tctcttcgat gccaccacca tcgcccgcct gggggagcag taccagctcc
    13801 tgctcgaatc cgccctggaa gaccccggcc ggcccatcgc cgagctgccg ctgctcgacc
    13861 ccgcccggcg gcatcagatg ctggtggagt ggaacggcgc cggcgtcccg gccaccgagc
    13921 cggtctgcct gcatgagcgc ttcgcggagc aggcggcggc gcggcccgac tccgtggccg
    13981 tgagcttcga ggacctctcc ctgacctatg gcgagctcgc cctccgcgcc cgccgtctcg
    14041 ccggccgtct tcgagcgttg ggagtaggac cggacgtgcg ggtgggggtg cggctggagc
    14101 ggcccgccga gatggtggtc tcgttgctcg gcgtgctgga ggcggggggc gcgtacgtgc
    14161 cgctggaccc gggatatccg gcggagcggc tggatttcat ggcccgggac gcggatctgc
    14221 gggtggtgat cgatcaggca tggatcgctc cggatggcgg cgatccgtcg aagccggagg
    14281 ccactcctcc cgacgcgctg gcgtacgtca tctatacgtc gggctccacc ggccggccca
    14341 agggggtggg catctcccac gcggcggcgg accgtctgtt cacggcgacg gttccctggt
    14401 tcggcttcgg ccccggagat gtctggaccc tctttcactc cttcgccttc gacttctcgg
    14461 tctgggagat gtggggggcc ctcctccatg gcggacgggt ggtggtggtt cccgaacggg
    14521 tccgccggtc tcccgaggag ctgctggacc tcctcgtgcg cgaaggggtg accgtgctcg
    14581 atcagacgcc gtcgagcttc cggcagctca tggccgtgga gggggagggt cccgcgctcc
    14641 ccctggcgct gcgctgggtg atcttcggcg gcgaggcgct ggacttccag agcctcgccc
    14701 cctggatggc gcgccacggc gacagccggc cgcggctggt caacatgtac ggcatcaccg
    14761 agacgacggt gcatgttacg tacagggagg tgactcttgg ggatctgcca gagattccgc
    14821 ggggtggtgg tgcctccccc cctgggaggg gggtggtagg gagcggggga ggacaggagg
    14881 ggggttttcc tggctcggca gccggcgagg ccgccggagc gtccgtgaaa actgcccccc
    14941 tcctaacctc ccccgctccc acccacgcgc ccgcccaggg ggggaggcac caccacccct
    15001 tgcggccaga gttgaacccc agcctcctcg gcgtcgccat ccccgacctc caggtctatc
    15061 tggcggaccg gggatcggcg ccggtgccga tcggggtgcc gggggaggtc tgggtcggtg
    15121 gcgcggggct ggcccgcggc tatctggggc ggccggacct caccgccgac cgtttccggc
    15181 cccacccctg gagcgagacg cccggcgcgc ggacctatcg ctcgggcgac ctggcgaggc
    15241 gccggccgga tggcgacctc gagtacctgg gacggatcga ccaccaggtg aagatccgtg
    15301 gctaccgggt cgagctgggc gaggtggagg cggccctgac cgcgttcccg ggggtgcggc
    15361 aggcggcagc cgtggtcagc gaggcccggc tgctggggta tgtggtgggg gagcccggtg
    15421 tccccctgga cctcgccgcc ctgcgcgcgt tcctggaagg gcgcctgccc ggaccgttcg
    15481 tcccgtcggc cctggtgccg ctcgcggccc tcccgatcac ccccggcggc aagctggacc
    15541 gcgcggccct ggcccaggcc ggaccggtgc ccgagcccgc cgcgcccatc tccgccccgg
    15601 ccgccgcagg accggtggcc gaggccctcg ccgggatctg ggccgaagtg ctcggcgtca
    15661 aggcagtggg tccgggagac gatttcttcc ggctgggagg gcactcgctg ctggcgaccg
    15721 ccgtcctgtc gcgggtgcgc cgggccttcc gggtcgagct gccggtggcg gcgctgttcg
    15781 aggcgccgac tctggcgggc ttcgcggcgc ggatcgagag cgagatgcgc ggcggcgaga
    15841 gcttcgaggg gccgcccctg gtggccaggc cccgctacgg cggcgccccc ctctcctact
    15901 cccagcggcg gctctggttc ctggaccgcc tggcgccggg caatccgttc tacaacgtgc
    15961 cggtggtgct ccgtctgcgc gggggcctcc gcccggcggc cctggaggcg gccctggcgg
    16021 agatcgtgcg ccgtcacgag atcctgcgct ccgccctgcc ggagctcgac ggcgagcccg
    16081 tgcaggccgc ggccccggct ctcgacgtgc gtctccccct cgtggacgtc tccgggctgc
    16141 ccgaggagat ccgggagggc gaggccgacc ggctggcgga tgaggagggg cggcgtccgt
    16201 tcgacctggc ggccgggccg ctcttccggt tctcgctcct gcggacggag cccggcgacc
    16261 accggctcct cctcacgctg catcacgtgg tgaccgacgc cggatccatg ggggtgctgc
    16321 gccgcgagtt ggagaccttc tatgaaggtg gggcttcact tcccgatcta cccctccagt
    16381 acgcggactt cgccctctgg cagcgcgact ggatcgccgc gggcggtatg gaggggcagc
    16441 tcgcctggtg gaaggaacgg ctggcgggtg ctcccgagga gccggggctg ccgttcgacc
    16501 gcccgcggcc ggcgcgggag cggttccgcg gcggcgccgt ccccctgcgc ctgccggtgg
    16561 cgacggccgc ggcggtccgt gccgcggccc ggcgtgaggg gatgacctcc ttcatggtcc
    16621 ttctcggagc gttccaggcc ctgctcgccc gcctcaccgg cgaggaggac gtggtggtgg
    16681 gcacgccggt cgccaaccgc gcccggcccg agatcgaggg gctcatcggc ttcttttcca
    16741 acaccctggt gctgcgcacc gacctctcgg ccgacccgga gggcgtcgag ctgctcgacc
    16801 gcgcccggca gaccgcgctc gccgcctacg cccaccagga cctgccgttc gaggtactgg
    16861 tggaggcgct ggcgcccgag cgccacctgg cccgcaaccc gctcttccag gtgctgctgg
    16921 tctatcagcg gctggagggg aagggcgggc tctccgatct cgccggcctg ctggtggacg
    16981 gggcccctcc ccagggtgcc gccggcaccg gctcggccaa gttcgacctc accctggcgc
    17041 tggtcgaggg ggaggagacc ttcgaggggg ccgtcgagta cgacgccgac ctgttcgatc
    17101 cccccacggc ggcgcggatc gccggccagc tcaccaccct gctggcggag ctggccgggg
    17161 actcgccgcc gcgcctcggc gatctccccc tgctcttggc acccgaacgg caccagctcg
    17221 ccgtcgagtg gaacgatgtt cgcgacgaga tgggactgca ggcagccgga tcgccggtcc
    17281 acgccggctt cgaggcctgg gccgggcggg agcccgcggc cccggcggtg gtctggaatg
    17341 attcgacgct cacctatggc gagctcgagc gcctctccgc cgcgctcgcc agccggctgc
    17401 ggga
//
LOCUS       DASPJI010000896         8765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_284375, whole genome shotgun sequence.
ACCESSION   DASPJI010000896 DASPJI010000000
VERSION     DASPJI010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8765)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8765
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284375"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VEL74_25395"
     CDS             complement(<1..411)
                     /locus_tag="VEL74_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004387140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HYG65938.1"
                     /translation="MIDTTTTAKILAGKDIAAAIRAEVAEEVARMTAAGHRKPALAAV
                     LVGGDPASHVYVNSKVKGCHEVGMASRAVLLPETTPEDEILRVLEELNEDDAVDGILV
                     QLPLPAGVNERRVVESVVAEKDVDGFHPENVGRLW"
     gene            complement(408..1136)
                     /locus_tag="VEL74_25400"
     CDS             complement(408..1136)
                     /locus_tag="VEL74_25400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HYG65939.1"
                     /translation="MSEAAAGVETKLDKSGRTIRDMFAGVAPRYDLLNHLLSANLDVL
                     WRKKATAVLGLPPGAEVLDLCCGTGDQATSLHRRGARVAAADFCIPMLAIARHKFARL
                     AGHRPGPLAADALALPFPDRRFDGATVSFGLRNVADLDRSLRELARVLKPGGKLVVLD
                     ATSPQGQPLRGLYLFYFRHLLPRIARILSPRSSAYQYLTESVLQFPQRQEFLARMAAA
                     GFTATAWQELSLGIVCVYSGKIPG"
     gene            complement(1220..1648)
                     /locus_tag="VEL74_25405"
     CDS             complement(1220..1648)
                     /locus_tag="VEL74_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014001683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HYG65940.1"
                     /translation="MRALLDINVLLALLDADHVDHQRAQEWISQEIQHGWASCALTQN
                     GFVRIISQPKYPSPISPSEAVERLRRATSTEYHEFWPCSISLLEERRINSSHVHGPRQ
                     VTDVYLLALAVEHGGRFVTFDRSIPLSAAPGATPEHLVVV"
     gene            complement(1648..1893)
                     /locus_tag="VEL74_25410"
     CDS             complement(1648..1893)
                     /locus_tag="VEL74_25410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HYG65941.1"
                     /translation="MRTTIDIADDVLMAVNERARRQKRSVGELLSELAREALTRRSRE
                     EPGAQKPKSLYGFDPLPPRGAAVSNELIDKLREEDFG"
     gene            2148..2594
                     /gene="rplM"
                     /locus_tag="VEL74_25415"
     CDS             2148..2594
                     /gene="rplM"
                     /locus_tag="VEL74_25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HYG65942.1"
                     /translation="MQNRTYSPKLAELQHDWFVLDATGIPLGRLSSLAARLLMGKHKP
                     TYVPHMDVGDFVIVINAEKAVLTGRKEEQKMYHHHTGYPGGLKSETAADRRRRRPTKM
                     VEDAVWGMLPKNRMGRHLLRKLKVYAGGEHPHQAQQPKPFDVAASL"
     gene            2632..3030
                     /gene="rpsI"
                     /locus_tag="VEL74_25420"
     CDS             2632..3030
                     /gene="rpsI"
                     /locus_tag="VEL74_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HYG65943.1"
                     /translation="MAANQNQFYGTGRRKTAAARVFMRQGAGEVKVNGRSLDEYFPNE
                     VLKMVIKQPLHLTETSEQFDILVTVAGGGSAGQAGAIRHGISRALIEYNAELRDRLKS
                     AGLLTRDPRKKERKKYGQKGARARFQFSKR"
     gene            3052..3822
                     /gene="rpsB"
                     /locus_tag="VEL74_25425"
     CDS             3052..3822
                     /gene="rpsB"
                     /locus_tag="VEL74_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HYG65944.1"
                     /translation="MVEVTMKELLEAGVHFGHQTRRWNPKMKPYIFGKRNGIYIIDLQ
                     KTVKLFDEVVKFVQELASQGKRILFVGTKRQAQEAIAEEAARCGEYFVTNRWLGGTLT
                     NFVTLRSSLQRLKEVEARLGDENSPMTKKERLRMERERDKMVKNLDGIRDMEELPDAL
                     FVIDPKKEDIAVAEANKLGIPVIAVVDTNCDPELIDYIIPGNDDAIRAIRLFAGRIAD
                     AYLAGSGKLQEEMMMDAKDGAALPMGTVDKDMAGAAAH"
     gene            3952..4605
                     /gene="tsf"
                     /locus_tag="VEL74_25430"
     CDS             3952..4605
                     /gene="tsf"
                     /locus_tag="VEL74_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HYG65945.1"
                     /translation="MEITAQMVKELRERTGAPMMDCKKCLGEAGGDLDKAADLLRKKG
                     IASADKKSGRVTAEGAVGSYIHGGGKIGVLVEVNCETDFVARTDDFQALVRDIAMHIA
                     AAEPRAVRREEVTQELLDREREIFREQALASGKPANVVDRIVEGKMEKYFSEFVLLEQ
                     PFVKDSDKTVGQMIAEKIGKMGENIQVRRFIRFKLGEGIEKRKEDFAAEVAAQVGRG"
     gene            4602..5357
                     /gene="pyrH"
                     /locus_tag="VEL74_25435"
     CDS             4602..5357
                     /gene="pyrH"
                     /locus_tag="VEL74_25435"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HYG65946.1"
                     /translation="MTEDSSPSDSSRVQGGPAFRRVLLKISGEALMGDKPFGLDEAVV
                     SAIADEIRDVHSLGVQIAIVVGGGNIIRGLAASHRGIDRVTGDYMGMLATVINGLALQ
                     DALEKRGVATRVQTAVDIREVAEPFIRRRALRHLEKGRAVIFAAGTGNPYFTTDSAAA
                     LRANEIHADVLLKATKVDGLYTADPMKDPNAVLLPEVTYQEVLEKDLQVMDTAAISLC
                     RDNGVPIVIFDLMAAGNIRRVICGERVGSIVRA"
     gene            5378..5932
                     /gene="frr"
                     /locus_tag="VEL74_25440"
     CDS             5378..5932
                     /gene="frr"
                     /locus_tag="VEL74_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014258400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HYG65947.1"
                     /translation="MKEVFKEVEQHMKHAVDHLHHELKHMRTGRASLGLLEGITVDYY
                     GSQVPLNQVANLQVADASLILATPFDPKQSGAIERAIMKSDLGLNPSSDGKVIRIPVP
                     QLTEERRKEIVKKAHDVAEHARTGVRQARREGNDKLKKMEKDKAISQDDERRGLDEVQ
                     KLHDRFIAEINTALEKKEQQIMQV"
     gene            5929..6663
                     /locus_tag="VEL74_25445"
     CDS             5929..6663
                     /locus_tag="VEL74_25445"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HYG65948.1"
                     /translation="MRGGPPSPPPMFLYGYHPVREALRHRPHEVARVLVSASRAGKRR
                     DEIEDLCARHRVAYQRVPEKTLSEIAGAAHNGMAAEVREAPGAAAERTGGDLELVVLL
                     EDVQDPRNLGALLRVCEGAGVGRVLIRDRGSAPVSPTVAKTSAGASEWLDVERITNSG
                     NEIERLKEEGYWVYGTDAAGDPPWDVDLSGKVVLCFGGEEKGLRARTRQLCDRMIGLP
                     MRGRVDSLNIATAAAAVLYDAVRQRS"
     gene            6741..7283
                     /locus_tag="VEL74_25450"
     CDS             6741..7283
                     /locus_tag="VEL74_25450"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HYG65949.1"
                     /translation="MPSVERIVQEETARATAESAHKNIETIVRLEEEEMKQRHLGERV
                     SDFFVRIMGSMSFVVAHLILFITWFSINLGFLGIEPFDPFPFGILTLIVSAEGVLVAL
                     FVLVSQNRMSRLANHRAHLNLQISLLAEQETTKLLQKVQSIVDRLGIETARDQELQRL
                     SQDTHLETLARELQEKLPAE"
     gene            complement(7325..7492)
                     /locus_tag="VEL74_25455"
     CDS             complement(7325..7492)
                     /locus_tag="VEL74_25455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65950.1"
                     /translation="MKKQVKKLTLAKETLGSLNRPDLVAGGTITKDTTCPCFEGSWCA
                     CSERNCSGWGC"
     gene            7625..7699
                     /locus_tag="VEL74_25460"
     tRNA            7625..7699
                     /locus_tag="VEL74_25460"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7657..7659,aa:Thr,seq:tgt)
     gene            7748..7834
                     /locus_tag="VEL74_25465"
     tRNA            7748..7834
                     /locus_tag="VEL74_25465"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7782..7784,aa:Tyr,seq:gta)
     gene            7885..7959
                     /locus_tag="VEL74_25470"
     tRNA            7885..7959
                     /locus_tag="VEL74_25470"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7917..7919,aa:Gly,seq:tcc)
     gene            8280..8354
                     /locus_tag="VEL74_25475"
     tRNA            8280..8354
                     /locus_tag="VEL74_25475"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8312..8314,aa:Thr,seq:ggt)
     gene            8415..>8765
                     /locus_tag="VEL74_25480"
     CDS             8415..>8765
                     /locus_tag="VEL74_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HYG65951.1"
                     /translation="MGKQKFERSKPHVNVGTIGHVDHGKTTLTAAITRVLASKGLADF
                     VAFDQIDKAPEERERGITIATAHVEYQSENRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTR"
BASE COUNT         1565 a         2897 c         2992 g         1311 t
ORIGIN      
        1 ccagaggcgg ccgacgttct cgggatggaa gccgtccacg tccttctcgg ccacgacgct
       61 ctccaccacc cggcgctcgt tcacccccgc gggcaagggg agctgcacca ggatgccgtc
      121 caccgcgtcg tcctcgttca gctcctcaag gacgcggagg atctcgtcct ccggcgtggt
      181 ctcgggaagc aggacggcgc ggctcgccat gccgacctcg tggcagccct tgaccttgct
      241 gttgacgtag acgtgcgagg ccgggtcgcc gccgacgaga acggcggcga gggcgggctt
      301 gcggtggccg gcggcggtca tccgcgccac ctcctcggcc acctcggcgc ggatggcggc
      361 ggcgatgtcc ttgccggcca ggatcttggc ggtcgtggtc gtgtcgatca tccggggatc
      421 tttccgctgt agacgcaaac gatgccgagc gagagctcct gccaggcggt ggcggtgaag
      481 ccggccgccg ccatgcgggc gaggaactcc tggcgctggg ggaactggag caccgactcg
      541 gtcaggtact ggtaggccga gctgcgcggc gagaggatgc gcgcgatccg cggcagcagg
      601 tgacggaagt agaagaggta gaggccgcgc agcggctgcc cctgtggcga cgtggcgtcg
      661 agcaccacca acttgccgcc gggcttgagc acccgcgcga gctcgcgcag cgaccggtcg
      721 aggtcggcga cgttgcgcag cccgaacgag accgtggcgc cgtcgaagcg gcggtcgggg
      781 aaggggaggg ccagcgcgtc ggcggcgagc gggccggggc gatgccccgc gaggcgcgcg
      841 aacttgtggc gggcgatggc gagcatgggg atacagaagt ccgccgccgc cacccgcgcg
      901 ccgcgccggt ggagcgacgt cgcctggtcg ccggtgccgc agcagaggtc gagcacctcg
      961 gcacccggag gcaatccgag gacggccgtc gccttcttgc gccagagcac gtccaggttg
     1021 gcggacagca ggtggttgag gaggtcatag cgcggcgcca cccccgcgaa catgtcgcgg
     1081 atggtccggc ccgacttgtc cagcttcgtc tcgactcccg cggcagcctc gctcacgcgg
     1141 gcaagtgtag cggatgcggg gggaagagag ggggggcggg gcggccaggc ggtcccggcg
     1201 ccccgagctg cggggtccct cagacgacga cgagatgctc cggcgtagct cccggagccg
     1261 cagacagggg gatcgaacgg tcgaaggtga caaaccgccc tccatgctcc accgcgagcg
     1321 ccagaaggta gacatcggtc acctggcgtg gcccgtgaac atggctggag ttgatgcggc
     1381 gctcctcgag aaggctgatg gagcagggcc agaactcgtg gtactcggtg ctggtcgctc
     1441 ggcgcagccg ctcgacggcc tcggaaggag agattggact ggggtacttg ggttggctga
     1501 tgatgcggac gaacccgttt tgcgtcaagg cgcaggaagc ccaaccatgc tgaatctcct
     1561 gcgagatcca ctcttgggct cgttggtggt cgacgtggtc tgcatccagt agcgcgagga
     1621 gcacgttgat gtcgaggagc gcccgcatca accgaagtct tcctcgcgga gcttgtcgat
     1681 cagttcattg gaaaccgccg cgccccgggg gggcaggggg tcgaaaccat acaggctctt
     1741 gggcttctgg gctccgggct cctctcggga ccgacgggtc aaagcctctc gggcaagctc
     1801 ggacaggagc tctccgacac tgcgcttctg gcggcgagcc ctttcattga ccgccatcag
     1861 aacgtcatcc gcgatgtcaa tggtggtgcg catgcatcag atgctagcac acgctatgtc
     1921 ccccttcccg ggggaatacc ccgctgttcc caaaggtggg caggtgcacg aggccccgtc
     1981 tcgtgctaga ctcgctgttc gctgcggcgg gagccgcggt ttctcggcgg ggctgccaaa
     2041 aagcccttga caatcccttt ttgtccgcct aatatcaggg gtcctttgcg tcgcgagggc
     2101 gggtttcctg cgccgaggcg gctttcccga gagttttgga gattgagatg cagaacagga
     2161 cctacagccc gaagcttgcc gagctccagc acgactggtt cgtgctcgac gcgacgggga
     2221 tcccgctggg acgcctgtcg tcgctcgccg cccgcctgct gatgggcaag cacaagccca
     2281 cctatgtgcc gcatatggac gtgggcgact tcgtcatcgt catcaacgcc gagaaggcgg
     2341 tgctgaccgg acggaaggaa gagcagaaga tgtatcacca ccacaccggc tatccgggtg
     2401 ggttgaagag cgagacggcc gccgaccggc gccgccgccg ccccaccaag atggtcgagg
     2461 acgcggtctg ggggatgctg cccaagaacc gcatgggccg tcacctgctg cgcaagctca
     2521 aggtctacgc cggcggcgag cacccgcacc aggcccagca gcccaagccc ttcgacgtgg
     2581 ccgccagcct gtaggcggcg ccggataccc ccagaggaac cgaggaagac gatggcagcg
     2641 aaccagaacc agttctacgg caccgggcgg cgcaagacgg cggcggcccg ggtgttcatg
     2701 cgtcagggcg ccggcgaggt caaggtcaac ggccgcagcc tcgacgagta cttccccaat
     2761 gaagtgttga aaatggtcat caagcagccc ctgcacctga cggagacgag cgagcagttc
     2821 gacatcctcg tcacggtcgc cgggggcggc agcgcgggcc aggcgggcgc gatccgccac
     2881 ggcatctccc gcgccctcat cgagtacaac gcagagctgc gcgaccggtt gaagtcggct
     2941 ggcctcctca cgcgcgatcc tcgcaagaag gagcgcaaga agtacggcca gaagggcgcc
     3001 cgcgctcgct tccagttcag caagcgctag tcggtacgaa gggagaatct cttggtcgaa
     3061 gtcacgatga aggagttgct ggaggctggt gtccacttcg gtcaccagac ccggcgctgg
     3121 aacccgaaga tgaagcctta catcttcggc aagcgcaatg ggatctacat catcgacctg
     3181 cagaagacgg tcaagctctt cgacgaggtc gtgaagttcg tccaggagct cgcctcgcag
     3241 ggcaagcgca tcctgttcgt cggcaccaag cggcaggcgc aggaggcgat cgccgaagag
     3301 gcggcgcgtt gcggcgagta cttcgtcacg aaccgctggc tgggcggcac gctcaccaac
     3361 ttcgtgacgc tgcgctcctc gctccagcgg ctcaaggagg tcgaggcccg tctcggcgac
     3421 gagaacagcc cgatgaccaa gaaggagcgc ctgcgcatgg agcgcgagcg cgacaagatg
     3481 gtcaagaacc tcgacggtat ccgcgacatg gaggagctgc ccgacgcgct cttcgtgatc
     3541 gatccgaaga aggaagacat cgcggtcgcc gaggccaaca agctgggcat cccggtcatc
     3601 gccgtcgtcg acaccaactg tgaccccgag ctgatcgact acatcatccc gggcaacgac
     3661 gacgcgattc gcgccattcg cctgttcgcc ggccgcatcg ccgacgccta cctcgccggc
     3721 tccggcaagc tgcaggaaga gatgatgatg gacgccaagg acggggccgc cctgcccatg
     3781 ggcaccgtcg acaaggacat ggccggcgcc gcggcgcact agacgagccc agatcgcacc
     3841 gccccctccc cgggggcggc gcccggaaaa ggtcagtcga acccgcgtcg atctgacctt
     3901 ttcttttgtc tgcacgattc cgagcacagc acgttcaagg agaagggtgt catggaaatc
     3961 accgctcaga tggtcaagga actgcgggag cgcacgggcg ccccgatgat ggattgcaag
     4021 aagtgcctcg gcgaggccgg cggcgacctg gacaaggccg ccgacctgct gcgcaagaag
     4081 ggcatcgcct ccgccgacaa gaagtccggc cgcgtcaccg ccgagggggc cgtcggctcc
     4141 tacatccacg gcggcggcaa gatcggcgtg ctcgtcgagg tcaactgcga gaccgacttc
     4201 gtcgcccgca cggacgactt ccaggcgctg gtgcgcgaca tcgccatgca catcgccgcc
     4261 gccgagcccc gcgccgtgcg ccgcgaggag gtcacccagg agctcctcga ccgcgagcgc
     4321 gagatcttcc gcgagcaggc gctcgcctcc ggcaagccgg ccaacgtcgt cgaccggatc
     4381 gtcgagggga agatggagaa gtacttctcc gagttcgtgc tcctcgagca gccgttcgtc
     4441 aaggattcgg acaagaccgt cggccagatg atcgccgaga agatcggcaa gatgggcgag
     4501 aacatccagg tgcggcgctt catccgcttc aagctcggcg aagggatcga gaagcgcaag
     4561 gaggacttcg ccgccgaagt cgccgcccag gtgggccgcg gatgacggag gactcctccc
     4621 cgagcgactc ctcccgagtc cagggcgggc cggctttccg gcgcgtgctg ctcaagatct
     4681 ccggcgaggc gctgatgggc gacaagccct tcggcctcga cgaggccgtg gtgtcggcca
     4741 tcgccgacga gatccgcgac gtgcactccc tcggggtgca gatcgcgatc gtcgtcggcg
     4801 gtggcaacat catccgcggc ctcgccgcga gccaccgcgg catcgaccgg gtgaccggcg
     4861 actacatggg catgctggcg acggtgatca acggcctcgc cctccaggac gccctggaga
     4921 agcgcggggt cgccacccgt gtccagacgg ccgtcgacat ccgcgaggtg gcggagccgt
     4981 tcatccgccg gcgggccctg cgccatctcg agaaggggcg ggcggtgatc ttcgccgccg
     5041 gcaccggcaa tccctacttc accactgaca gcgcggcggc tctccgggcg aacgagatcc
     5101 acgccgacgt cctgctcaag gccaccaagg tggacgggct ctatacggcc gacccgatga
     5161 aggacccgaa cgcggtgctg ctccccgagg tcacctacca ggaggtgctg gagaaggact
     5221 tgcaggtgat ggacaccgcg gccatcagcc tctgccggga caacggcgtg cccatcgtca
     5281 tcttcgacct catggctgcc ggcaacatcc ggcgggtcat ctgcggcgag cgcgtcggct
     5341 ccatcgtgcg ggcataaaat aggcccgagg agggaccatg aaagaggtgt tcaaggaagt
     5401 cgagcaacac atgaagcacg cggtggacca cctccaccac gagctgaagc acatgcggac
     5461 cgggcgggcc tcgctggggc tgctcgaggg gatcaccgtc gactattacg gcagccaggt
     5521 gccgctcaac caggtcgcga acctccaggt agcggacgcc tcgctgatcc tcgccacccc
     5581 cttcgatccg aagcagagcg gcgccatcga gcgggcgatc atgaagtccg acctcgggct
     5641 caacccgtcg tcggacggca aggtgatccg catcccggtg ccgcagctca ccgaggagcg
     5701 gcgcaaggag atcgtcaaga aggcccacga cgtcgccgag cacgccagga ccggcgtccg
     5761 ccaggcccgc cgcgagggca acgacaagct caagaagatg gagaaggaca aggccatcag
     5821 ccaggacgac gagcgccgcg gcctggacga ggtccagaag ctccacgacc gcttcatcgc
     5881 cgagatcaac accgctctgg agaagaaaga gcagcagatc atgcaggtgt gaggggcgga
     5941 cccccctcgc ctcctcccat gttcctctac ggctaccatc ccgtccgcga agccctgcgc
     6001 caccgtcccc acgaggtggc gcgggtgctg gtgtccgcct cccgggcggg caagcggcgc
     6061 gacgagatcg aggatctgtg cgcgcgccac cgggtcgcct accagcgggt gcccgagaag
     6121 accctgtccg agatcgccgg cgccgcccac aacggcatgg ccgccgaggt gcgcgaggcc
     6181 cccggcgcgg cggccgagcg gaccggcggc gatctcgagc tcgtggtgct cctggaggat
     6241 gtccaggacc cccgcaacct cggggcgctg ctgcgcgtct gcgagggggc cggcgtcggg
     6301 cgggtgctga tccgcgaccg cggctcggcg ccggtgtcgc ccaccgtggc caagacctcc
     6361 gccggcgcca gcgagtggct cgatgtcgag cgcatcacca actccggcaa cgagatcgag
     6421 cgcctcaagg aggagggcta ctgggtctac ggcaccgacg cggcgggcga tcccccgtgg
     6481 gacgtggacc tctccggcaa ggtcgtcctc tgcttcggcg gcgaggagaa ggggctgcgg
     6541 gcccgcaccc gccagctctg cgaccgcatg atcggcctgc ccatgcgcgg ccgcgtcgat
     6601 tccctgaaca tcgccaccgc cgcggccgcc gtcctctacg acgcggtacg ccagcgaagc
     6661 tgaagcctct ccccccccgg cacgccgctc gcaaggagca gcagcaggaa ctctcctcgt
     6721 ttccggagag gcttgtggag atgccgagcg tagaaagaat cgtccaggaa gagaccgcca
     6781 gggccaccgc cgagtcggcc cacaagaaca tcgagaccat cgtccgcctc gaagaggagg
     6841 agatgaagca gcggcatctg ggcgagcggg tgagcgactt cttcgtccgc atcatgggga
     6901 gcatgtcgtt cgtcgtcgcc cacctgatcc tgttcatcac ctggttctcg atcaacctgg
     6961 gcttcctggg catcgagccc ttcgacccgt tccccttcgg catcctgacc ctgatcgtgt
     7021 cggccgaggg ggtgctggtg gcgctcttcg tcctggtcag ccagaaccgg atgagccgcc
     7081 tggccaacca ccgggcgcac ctgaacctcc agatcagcct gctggccgag caggagacca
     7141 ccaagctcct ccagaaggtg cagagcatcg tcgaccggct ggggatcgag accgcgcgcg
     7201 accaggagct gcagcggctg agccaggaca cccacctcga gaccctcgcc cgggagctgc
     7261 aggaaaagct gccggcggag tgaggcccgc cggggccggc gggcctcacc tctcgggccg
     7321 gggctcagca gccccagccg ctgcagttgc gttcgctgca ggcgcaccag ctcccctcga
     7381 agcaggggca ggtggtgtcc ttggtgatcg tgccgccggc gacgaggtcc ggacggttca
     7441 gggagccgag agtctccttg gccagggtga gcttcttgac ctgcttcttc atgcgcgcct
     7501 cctcgaggtg gaatgacgag gtcgcccgcc gcgttgtgcg gagggccctc tagaagttgg
     7561 tgcgcggggg cgttgcatgc gggcacgcta gcggctacac tatcgcccct ggcccggggg
     7621 acccgccggc atagctcagg ggtagagcta ctgtcttgta aacagtaggt ccccggttcg
     7681 aatccgggtg ccggctccag tgatgggtac ggcgccgggc ttgaagcagc actttgacag
     7741 cagcactgga ggtgtggccg agtggacaat ggcagcagac tgtaaatctg ccgggcttcg
     7801 ccctacggag gttcgaatcc tcccgcctcc accattccct cgctgccgtc agcgcccgcc
     7861 agcaccagta tcgcgagctc ccgagcggga gtaactcagg ggtagagtca cagccttcca
     7921 agctgttggt cgcgggttcg aatcccgtct cccgctccat tcgacctccg tccctcatca
     7981 gcccatccct gaccagcgcg ggaatagaga cggcggcggt gcgtgtttct tttcacggcc
     8041 tgccggaagg ggctggacat ccgaaaagat ctctggcaca atcttcgtcc ctctcctccg
     8101 ggcgcctgtt cccgggcgtc cgcaccgggc tccccttcgg cgatggaaag tcgatggcga
     8161 cgggttggtt ccagcggaga tcggggcttt acaggcaccg gggagattgg taggatcatc
     8221 cggtttgacc gtggacgccc tccgaggcac cggccccggg ggagtctagg agtgtctaag
     8281 cccacgtggc tcaggggtag agcacttcct tggtaaggaa ggggtcaccg gttcaaatcc
     8341 ggtcgtgggc tccataaatt tcgacgcaag ttcgcgtgaa caaatcagcg ctttcaggag
     8401 gcatctgaca cgccatgggt aagcagaagt tcgagcggtc caagccgcac gtcaatgtcg
     8461 gtacgatcgg tcacgtcgac catggcaaga cgacgctgac cgcggcgatc acgcgggtgc
     8521 tggcctccaa gggcctcgcc gacttcgtgg cgttcgacca gatcgacaag gcgccggagg
     8581 agcgggagcg cggtatcacc atcgccaccg cccacgtgga gtatcagagc gagaaccggc
     8641 actacgccca cgtcgactgc ccgggccacg ccgactacgt gaagaacatg atcaccggcg
     8701 cggcgcagat ggacggcgcc atcctggtgg tttcggcggc cgacggcccg atgccgcaga
     8761 cgcgg
//
LOCUS       DASPJI010000897         3830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_512557, whole genome shotgun sequence.
ACCESSION   DASPJI010000897 DASPJI010000000
VERSION     DASPJI010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512557"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VEL74_25485"
     CDS             <1..732
                     /locus_tag="VEL74_25485"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HYG65952.1"
                     /translation="RTKLVAVAHVSNALGTINPVRQIADLAHAHGAVVLVDGAQGVPH
                     LEVDVQALGCDFYAFSGHKVYGPSGIGVLWGRGELLAGMPPWQGGGGMISSVTFEETT
                     FAPPPERFEAGTPFIEGAIALGTALDYLTAVSLPAVAAWETELMSLATERLAEIPGLR
                     FVGTARHKAAVLSFAMEGIHPHDVGTILDREGIAVRAGHHCAQPVMQCFGVPATSRVS
                     FSLYNTAAEVDALAAGLHKVREVFG"
     gene            732..1184
                     /locus_tag="VEL74_25490"
     CDS             732..1184
                     /locus_tag="VEL74_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HYG65953.1"
                     /translation="MSSDLRDLYQEVILDHNRRPRNFGTLASANRTAEGHNPLCGDRV
                     RLFVDVEEDRVRDIAFEGSGCAISTASASLMTEAVKGKTVEEARELFRLFHDLMTAEG
                     ADADDPSLGKLAVFAGVREYPIRIKCATLAWHAFQAALANRVQPVVTE"
     gene            1204..1620
                     /locus_tag="VEL74_25495"
     CDS             1204..1620
                     /locus_tag="VEL74_25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HYG65954.1"
                     /translation="MDEHQNDTTATAVADTPEATPERTPEAAPEAIAGPIDTAALENQ
                     IVEQLQTVFDPEIPVNIYELGLIYDVDIQPGGDVHIKMTLTSPACPVAGSLPGEVQRK
                     VEEVPGVASADIELVWDPAWNPSMMSEAARLQLGMF"
     gene            1679..2200
                     /locus_tag="VEL74_25500"
     CDS             1679..2200
                     /locus_tag="VEL74_25500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG65955.1"
                     /translation="MRQPDPADDPGPAIQELVGRLRPKIKRILCLYNIPSVDAEDILQ
                     EALIAAFRKWDSIQHKDAWLLGTVRNRCMLYWKHRWRERSEGMDPEDLEAVSSPVPPP
                     QERAEMLWDLEVIAETLGERHWAMLCLRYGLGLSIPEVARRLGYCPSSIRKLSCRVVN
                     RLQRQMAVTLKEP"
     gene            2312..3574
                     /locus_tag="VEL74_25505"
     CDS             2312..3574
                     /locus_tag="VEL74_25505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HYG65956.1"
                     /translation="MRAHTNNNSVVMRAVEDLPREAEEEKPPARPGEPRLVRSGEDLP
                     GQRQKMEAMGRLAGGIAHVFNNLLTAIACETELALVKLAQDDPARKHLREIEKVGERG
                     ASLARQLLAFSGRQVLHPKMVHLNSLLSDLDEELRRILGDAIELRLELASDLDRINVD
                     PAEFRQAILSLASNARDVMPDGGRLTLETKNLEVRPGNLAHPLRTSPGHYVLLEVRDT
                     GPGMDEDVRRRIFEPFFTTKKGAEIVGLGLSIAYGIITQSGGHVLVDSEPGKGSRFLI
                     FLPSAEEAGSRTADTAREARHRETLLLVEDEENVRKPLRQIMEARGYHVLEASGADEA
                     MAISQEHQGPIHIMVTDILMDGMNGVELAERLAFKRPEMKVLFATGYPAGLAERPSLT
                     NEEAPLLKKPFSGKDLAAKVREVLESSD"
BASE COUNT          634 a         1356 c         1301 g          539 t
ORIGIN      
        1 cgcacgaagc tggtggcggt ggcgcacgtc tcgaacgccc tggggacgat caacccggtc
       61 cgccagatcg ccgacctggc ccatgcccac ggcgcggtgg tgctggtcga cggcgcccag
      121 ggggtcccgc acctcgaagt ggacgtacag gcgctgggct gcgacttcta cgccttctcc
      181 ggccacaagg tctacggtcc gagcggcatc ggcgtcctct gggggcgggg cgagctgctc
      241 gccggcatgc ccccctggca ggggggcggc gggatgatct cctccgtcac cttcgaggag
      301 accaccttcg cgccgccgcc cgagcgcttc gaggccggca cgccgttcat cgagggggcc
      361 atcgccctgg ggacggcgct cgactacctg acggcggtga gcctcccggc cgtcgccgcc
      421 tgggagaccg agctgatgag cctggcgacg gagcggctgg cggagatccc cggcctgcgc
      481 ttcgtcggca ccgcccgcca caaggcggcg gtcctctcct tcgcgatgga agggatccac
      541 ccgcacgacg tcggcaccat cctcgaccgc gaggggatcg cggtgcgcgc cggccatcac
      601 tgcgcgcagc cggtcatgca gtgcttcggc gtccccgcca ccagccgggt ctccttctcc
      661 ctctacaata cggccgcgga ggtcgacgcc ctggcggccg gcctccacaa ggtacgggag
      721 gtcttcggct gatgtcgtcc gacctgcgcg acctctacca ggaggtcatc ctcgaccaca
      781 accgccggcc ccgcaacttc gggacgctgg cgtccgccaa ccgcacggcg gagggccaca
      841 acccgctgtg cggcgaccgc gtgcggctgt tcgtcgacgt cgaggaggac cgcgtccgcg
      901 acatcgcctt cgagggctcg ggctgcgcca tctccaccgc ctcggcgtcg ctgatgacgg
      961 aagcggtcaa gggcaagacc gtggaggagg cgcgggagct gtttcgtctc ttccacgacc
     1021 tcatgacagc cgagggcgcc gacgcagacg atccgtccct cggcaagctc gcggtcttcg
     1081 ccggggtgcg ggagtacccc atccgcatca agtgcgccac cctcgcctgg cacgccttcc
     1141 aggccgccct cgcgaaccgg gtgcagcccg tcgtcaccga ataggcaccg agtaggagct
     1201 tccatggacg agcaccagaa cgacaccacc gccacggccg tcgccgacac cccggaggca
     1261 actccggagc gcaccccgga ggccgctccg gaggccatcg ccggcccgat cgacaccgcc
     1321 gccctggaga accagatcgt cgagcagctc cagacggtgt tcgacccgga gatcccggtc
     1381 aacatctacg agctggggct gatctacgac gtcgacatcc agccgggcgg cgacgtgcac
     1441 atcaagatga cgctcacctc ccccgcctgc ccggtcgccg gctcgctgcc cggtgaggtc
     1501 cagcgcaagg tcgaagaggt gcccggagtt gcgtccgccg atatcgagct ggtctgggac
     1561 cccgcctgga acccctcgat gatgtcggaa gcggcgcgcc tccagctcgg gatgttctag
     1621 agccgcgacc cgcgttctcg tctgaagtga taaagtcgta cgggtgaaca ctgcacccgt
     1681 gcgacagcct gacccggcgg acgaccccgg gccggcgatc caggagctcg tcggccggct
     1741 ccgccccaag atcaagcgca tcctctgcct ctacaacatc cccagcgtgg acgccgagga
     1801 catcctgcag gaggccctca tcgcggcctt ccgcaagtgg gactcgatcc agcacaagga
     1861 cgcctggctc ctcgggacgg tccgcaaccg ctgcatgctg tactggaagc accgctggcg
     1921 cgagcggtcc gaggggatgg accccgagga cctggaggcg gtcagctcgc cggtcccgcc
     1981 gccgcaggag cgggcggaga tgctatggga cctggaggtg atcgccgaga ccctcggcga
     2041 gcgccactgg gcgatgctct gcctgcgcta cggcctgggc ctctccatcc ccgaggtggc
     2101 ccggcgtctc ggctactgcc cgtcgagcat ccgcaagctg tcctgccgtg tcgtcaaccg
     2161 cctccagcgg cagatggcgg tcaccctgaa ggaaccctga aggaacccgc cggggctgga
     2221 ccgaaacctg catatctctc cgccaagggc tttgccgcgg cgtacggcgt ctttcgcccg
     2281 gaacagaaag aaagccgagg gacaagagag tatgcgagca catacgaaca acaatagtgt
     2341 ggtgatgcgg gctgtcgagg acctgccgag agaggccgaa gaagagaagc ctccggcccg
     2401 ccccggggag cctcgcctgg tgcggagcgg cgaggatctg cccgggcaga ggcaaaagat
     2461 ggaggccatg gggcgcttgg cgggtggaat tgcgcatgta ttcaacaacc tgctcaccgc
     2521 catcgcctgt gagaccgagc tggccctggt gaagctggcc caggacgacc ccgcccgcaa
     2581 gcacctgcgg gagatcgaaa aggtcggcga gcgcggagcc tccctcgccc ggcagctcct
     2641 ggcgttcagc ggccggcagg tgctgcaccc caagatggtg cacctcaaca gcctgctgtc
     2701 ggacctcgac gaggagctgc ggcgcatcct gggcgacgcc atcgagctcc ggctggagct
     2761 cgcgtccgac ctggaccgca tcaatgtcga cccggcagag ttccggcagg cgatcctcag
     2821 cctggcgtcg aacgcccggg acgtgatgcc ggacggcggg cggctcaccc tggagaccaa
     2881 gaacctggag gtgcggccgg gcaacctcgc ccatccgctg cgcacctcgc ccggccacta
     2941 cgtgctgctg gaggtacgcg acaccgggcc aggcatggac gaggacgtcc ggcggcgcat
     3001 cttcgagccg ttcttcacca ccaagaaggg ggccgagatc gtcggcctgg ggctctccat
     3061 cgcctacggg atcatcaccc agagcggcgg ccacgtgctg gtggacagcg agccgggcaa
     3121 ggggtcgcgc ttcctgatct tcctgcccag cgccgaggag gcggggagcc gcacggcgga
     3181 cacggcgcgc gaggcgcggc accgcgagac gctcctgctg gtggaggacg aggagaacgt
     3241 gcgcaagccg ctccgccaga tcatggaggc ccggggctat cacgtgctcg aggcctccgg
     3301 cgccgacgag gcgatggcga tcagccagga gcatcaaggg ccgatccaca tcatggtcac
     3361 cgacatcctg atggacggca tgaacggcgt cgagctcgcc gagcggctgg cgttcaagcg
     3421 ccccgagatg aaggtgctgt tcgccaccgg ctatccggcc ggcctggccg agcggccgag
     3481 cctgaccaac gaggaggcgc cgctgctcaa gaagccgttc agcggcaagg acctcgccgc
     3541 caaggtgcgc gaggtcctcg agagctcgga ctagacgttt tcgacgagga gagggagctc
     3601 ttttgagcgg cccgccccca cccgggggcg ggccgcgtct acttccggga agtgcttcag
     3661 ggcagcgtct cctcgtggag gttgcccaga tagagggcgt tccacagcag cttgaaggtg
     3721 cggtgcggct gcgcccggtg ctgcggccgg aagccgatca gcaccaccct ccccttgccg
     3781 accgtgaagt cgaccaccgc cgcctgcttc tccagccgct cgcccccctt
//
LOCUS       DASPJI010000898         8577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_512893, whole genome shotgun sequence.
ACCESSION   DASPJI010000898 DASPJI010000000
VERSION     DASPJI010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8577)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8577
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512893"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..6490
                     /locus_tag="VEL74_25510"
     CDS             14..6490
                     /locus_tag="VEL74_25510"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65957.1"
                     /translation="MVVTEQRLLASLPPEARRVVCLDVEQAALSAESAARPRPLAGPQ
                     NLAYVIYTSGSTGRPKGVAISHVSASLLVQWAGETFGADELSGVLASTSLSFDLSVFE
                     LFVPLSWGGTVILAPNVLHLPALPAAARVRLINTVPSALAELVRAESLPPGVKTVNLA
                     GEPLARGLAQQILASGTVERLWNLYGPSEDTTYSTAARVEPGERAPSIGRPIDGGQSW
                     VVDGGLRPVPVGVAGELLLGGEGLARGYLSRPDLTAERFVPDPLGGEAGARLYRTGDR
                     ARWRRAGELEFLGRLDHQVKVRGFRIELGEIESVLSSAAGVRESVVLARPDGPGGPRL
                     VAYVVPAAGEKPAATELRELLQQRLPDYMVPAVFVTLDSLPLTPNSKVDRRALPAPAG
                     LHPAPGTAYLAPRTPGEEILASIWAEVLGVERVGLRDNFFELGGHSLLATQVISRVRE
                     AFGVELPVRELFEEPTVEGLAGRIERLRESAAPAPPIVPVPRGEGVLPPSFAQQRLWF
                     VDRLKPGSSAYNVPLAARLTGALDAAALGSALREVACRHEVLRTTFAERDGRPVQVIA
                     PEPRLPLDIVDLRGLPEAEREAEVARRAITETLRPFDLAAGPLIRAALLRLAEEEHAL
                     LVTMHHIVTDGWSMGIFLRELTELYGGRELPVLPVQYADFAVWQRSWMTGEVLESQLS
                     YWRGHLAGAPARLTLPTDRPRPAVTTNHGTNQPVVLPPSLSRALSALARREGATPYMV
                     LLAGFAALLGRYSGQRDVVVGSPIAGRNRRETEGLIGLFVNALALRVELAEGLDFLSL
                     LRQARRTALDGFAHQDIPFERLVEELVSERDLGHSPLFQVTLALQNVPSSATAVSGLA
                     IRGVAMESRTAKFEMSLRLVPVGDELAGGLEYNTDLFDAATAERLAGHLARLLELAVA
                     APEVPVPDLGLLAPAERQHLLREWNDTGMDYGAESGLCLHDLIGMQVERTLDAVAAVS
                     GDEHLSYHELAQQAGRLARRLAAVGVGADSVVGICAERSLEMIVGLLGVLEAGGSYLP
                     LDPSYPRERLATILESAGAEAVLIQEPLTDRLPEMSIPRLPLRPARDPVSWTPPAIRP
                     ENLAYVIFTSGSTGAPKGAMVSHQAIVNRLLWMQAAYGLSPADRVLQKTPFGFDVSVW
                     EFFWPLLVGAPLVFARPEGHKDPAYLASLIAEQGITVLHFVPSMLRAFLETDRRESCR
                     SVRAVMASGEALPFDLTERFFASMPGELHNLYGPTEAAVDVSFWPCVPGDDRRLVPIG
                     RPIANLRLHVAERSWQPAPLGVAGELLIGGMGLGRGYLGRPDLTAERFVPDPFGSEAG
                     GRLYRTGDLARHLPEGEIEFLGRLDFQVKIRGFRIELGEIEEALLAHDQIREAVVLVR
                     PVAGSGEGSQERRLVAYVVSETGEEPADLRGFLGQRLPDYMVPPVVVVLPALPLSANG
                     KIDRAALPAPEMANAVAEHVPPRTALERFLASGFRDVLALPPERPVGLHDDFFALGGH
                     SITGAIFINRLQETLSEIVHVVTLFDHPTVASLAAYVTGQHPQAARRLWGEPATVESD
                     VVAQVGPAEIEEMRRLVVTGRPEVPEEVQAPAPPNPPAVFVLSPPRSGSTLLRVMLGG
                     HPGLFAPPELELLSFPTLSQRRQAFQGRDSFWLEGVVRAVMEARGCGAEEAERLIAGY
                     EDGGWSTRRFYGELQSWLGERLLVDKTPSYALDPEVLARAESWFTAPRYIHLMRDPRA
                     TNLSFEEAKLDQIFFRRPHRFTRHQLAELFWTVCHRNILDFLSALPRERWLPVRFEEL
                     VREPERVLSGICDFLGIDYRPEMADPYREGRRKGRRSRMTDGVHDVSRMLGDVKFHTH
                     SRVDSSAAERWRQAEEVPLGTPARELALELGYADAVPELVAQTDILVRLQAGAPEVRP
                     LFCVHPVGGDVACYRELARRLGPDQPVFGLRSPQEPIPDLRQMATLYVEVARQAQPAG
                     PYRLAGWSMGGLVAYEMARQLEEQGEAVELVALIDAASPQWWAEQTPSSDGELVAWFA
                     RDLARLFGAAQVPAVRLDAPDVDGNLARVLEAGHATGLVAAGVGLADLRRLFEVFRAN
                     RIALSSYPPLPYSGALELIRAAETGGEEGEDPYRGWSGLAAGGVTVHTVQGDHYSIVL
                     GEGVERLTGLLRSWLSGPKDGELSHG"
     gene            6593..8551
                     /locus_tag="VEL74_25515"
     CDS             6593..8551
                     /locus_tag="VEL74_25515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HYG65958.1"
                     /translation="MYKISLINMPFANLGLPSIALTQLKSIAEQRFGGDVRVRILYLN
                     LNFSQYLGTDVYGHITGALQANNSGLGDWLFRQIAFPNQPDNTASYFQRYFPRMDAET
                     SVVKGSILAKRAGLKRYFERLVTQYQLDREDLVGFSSMFAQNVASMAMARVIKDRNAA
                     VITVMGGANCESPMGEELVRHVEALDFVFSGPALISFPDLVAAQLAGDRDRCHQIRGV
                     FSRQNVGAPHMQGHGAMGAELPIETPVPLDYESFLDDLSRSFPDGSVQPSLTFETSRG
                     CWWGERSHCTFCGLNGGTMMYRAMPSDQALALFREIFEKYGDRCTNIFSVDNIMPRQY
                     IKEVFAHLVPPEGTSIFYEVKADLKEWEMEVLSRAGVTEIQPGIEALATSTLKLMGKG
                     TTSFQNVAFLKSCLRYGIHPAWNLLIGFPREEETVYEGYLRALPLLMHLPPPAGAFPI
                     RFDRYSPYYTRAAEYGLDLAPYAFYGAIYPFGEEALSRMAYYFEDRHYDADYLVRLLR
                     WQERLNDTVQRWVQRWEGRDGALRAELRLKMDGDGAVVHDTRTGERVEHRLSALGLDV
                     LHAARGRGLGARHLRDQMGIDEPTAVAELARLAELGLVFEEDGRFIGLVMDIEARAPD
                     LALQGITSVAEDERLASAAGGDRREVQP"
BASE COUNT         1176 a         2760 c         3255 g         1386 t
ORIGIN      
        1 gtcggcggcc ctggtggtgg tgaccgagca acggctcctg gcgagcctgc cgcccgaggc
       61 gcggcgggtg gtctgcctcg acgtcgagca ggcggcgttg tcggccgaga gcgccgcccg
      121 cccgcggcct ctggccggcc cgcagaatct agcctacgtg atctatacgt cggggtcgac
      181 agggcggccg aagggggtgg cgatctccca tgtcagcgcg tcgctgctgg tgcaatgggc
      241 cggcgagacc ttcggtgccg acgagctgtc gggagtgctg gcgtcgacct cattgagctt
      301 cgacctgtcg gtgttcgagc tgttcgtgcc gctgtcgtgg ggcggcacgg tgatcctggc
      361 gccgaacgtg ttgcacctgc cggctttgcc ggccgcggcg cgggtgcgcc tgatcaatac
      421 ggtgccttcg gcgctggcgg agctggtgcg ggcggagtcg ttgccgccgg gtgtgaagac
      481 ggtcaacctc gcgggcgagc ctttggcgcg cggtctggcg cagcagatcc tggcgagcgg
      541 cacggtcgag cggctgtgga acctgtacgg tccgtcggag gacacgacgt attcgaccgc
      601 cgcgcgggta gagcccgggg agagggcgcc ctcgatcggc cggccgatcg acggggggca
      661 gtcgtgggtg gtggacggcg gcctgaggcc ggtgccggtg ggagtggcgg gagagctgct
      721 cctcgggggc gagggtctgg cgcgagggta tctgagccgg ccggacctga cagcggagcg
      781 gttcgtgccg gatccgctgg ggggcgaggc cggagcgcgg ctctaccgga ccggagaccg
      841 ggcgcgctgg cggcgcgccg gcgagctgga gttcctgggc cgcctcgatc atcaggtcaa
      901 ggtgcgcggc ttccgcatcg agctcggcga gatcgagtcg gtgctgtcgt cggccgccgg
      961 ggtgcgggag tcggtggtgc tggcacggcc ggacgggccg ggcgggccgc gtctggtggc
     1021 ctatgtggtg ccggcggcgg gcgagaagcc ggcggccacg gaactgcggg agctcctgca
     1081 gcagcggctg ccggactaca tggtgccggc ggtcttcgtg accctcgaca gcctgccgct
     1141 gacgccgaac agcaaggtgg accgccgggc gctgccggcg ccggcaggtc tgcaccccgc
     1201 tccaggtacg gcctacctgg cgccgcggac tccgggggag gagattctgg cgtcgatctg
     1261 ggccgaggtg ctgggagtcg agcgcgtggg cctgcgggac aacttcttcg agctgggcgg
     1321 ccactcgctg ctggcgaccc aggtgatctc ccgtgtgcgc gaggccttcg gcgtggagct
     1381 gccggtgcgc gagctgttcg aggagccgac ggtggagggg ctggccgggc ggatcgagcg
     1441 gctgcgcgag tccgcggcgc cggcgccgcc gatcgtgccg gtgccccgag gcgagggcgt
     1501 cctgccaccg tcgttcgccc agcagcgcct ctggttcgtg gaccggctca agccgggcag
     1561 ctccgcctac aacgtgccgc tggcggcgcg gctcaccggc gcgctcgatg ccgcggcact
     1621 cggcagcgcg ttgcgcgagg tggcgtgccg ccacgaggtg ctgcgcacca cgttcgccga
     1681 gcgggacggc cgtcccgtgc aggtgatcgc gcccgagccg cgcctgccgc tcgacatcgt
     1741 ggacttgagg ggcctgccgg aggcggagcg cgaggccgag gtggcgcgac gggcgatcac
     1801 ggagacgctg cggccgttcg acttggcggc cgggccgctc atccgggcgg cgctgctgcg
     1861 gctggccgag gaggagcacg cgctgctggt gaccatgcac cacatcgtca ccgacggctg
     1921 gtccatgggc atcttcctgc gcgagctgac ggagctgtac ggcggccgcg agctgccggt
     1981 gctgccggtg cagtacgcgg actttgcggt ttggcagcga tcttggatga ccggcgaggt
     2041 actggagtcg caactgtcct attggcgggg gcacctggcc ggggcgccgg cccgtctgac
     2101 cctgcccacc gaccggccgc ggccggcggt gacgaccaat catgggacca accagccggt
     2161 ggtactgcct ccatccctct cgcgggcgct gtcggcgctg gcccggcgtg agggcgcgac
     2221 gccgtacatg gtcctcttgg ccgggttcgc ggcgctgctg ggccgctact ccgggcagcg
     2281 tgacgtggtg gtgggctcgc ccatcgccgg ccgcaacagg cgggagaccg aggggctcat
     2341 cgggcttttt gtcaacgccc tggcgttgcg ggtagagctg gcggaggggc tcgatttcct
     2401 gagtcttttg cggcaggcgc ggcggacggc actggatggc ttcgcccacc aggacatacc
     2461 gttcgagcgt ctggtcgagg aactggtgag cgagcgcgac ctggggcact caccgctgtt
     2521 ccaggtgacg ctggcattgc agaacgtgcc gtcgtcggcg accgcggtct ccggactggc
     2581 gatacggggc gtggcgatgg agtcgcggac ggcgaagttc gagatgagcc tgcgtctcgt
     2641 cccggtaggg gacgagctgg ccggcggcct ggagtacaac accgacttgt tcgacgcggc
     2701 cacggcggag cgcttggccg ggcatctggc ccgattgctg gagctggcgg tcgcggcgcc
     2761 ggaggtgccg gtgccggacc tcggtctgct ggccccggcc gagcggcagc acctgctgcg
     2821 ggagtggaac gacaccggga tggactatgg ggcggagtcc ggactctgtc tgcacgatct
     2881 gatcgggatg caggtggaac gcacgctaga tgctgtggcc gcggtttccg gagatgagca
     2941 cctgagctat cacgagctgg cccaacaggc cggccgcctg gcccggcgcc tggccgcggt
     3001 gggggtcggg gcggattcgg tggtgggaat ctgcgccgag cggtcactgg agatgatcgt
     3061 gggcctgctc ggagtgctag aggccggcgg ctcctatctg ccactagacc cgtcgtaccc
     3121 acgggagcgg ctggcgacca tcctggagag cgccggggcc gaggccgtgc tgattcagga
     3181 gcctctgacg gaccgtctgc ccgagatgtc catcccgcgg ctgccgctga ggccggcccg
     3241 tgatcccgta tcctggacgc ctcccgcgat ccggcctgag aacctggcct acgtcatctt
     3301 cacctcgggc tcgaccggcg cgcccaaggg ggcgatggtg tcgcaccagg caatcgtcaa
     3361 ccgcctgctc tggatgcagg cagcctacgg cctgagcccg gccgaccggg tgctgcagaa
     3421 gacgccgttc ggcttcgacg tctcggtctg ggaattcttc tggccgctgc tggtgggagc
     3481 gccgctggtg ttcgcccgtc ccgaaggcca caaggacccc gcctacctgg cgtcgctgat
     3541 cgcggagcag gggatcacgg tgctgcactt cgtgccgtcg atgctgcggg cgttcctgga
     3601 gacggaccgg cgggagagct gtcggtcggt ccgggcggtg atggccagcg gcgaagcatt
     3661 gccattcgat ctgaccgagc gcttcttcgc gtcgatgccg ggggagctgc acaacctcta
     3721 cggcccgacc gaggcggcgg tggacgtcag cttctggccc tgcgtgccgg gagacgaccg
     3781 gcggctggtg ccgatcggcc gtccgatcgc caacctgcgc ctgcacgtgg cggagcggag
     3841 ctggcagccg gcgccgctgg gagtggccgg cgagctgctg atcggtggta tgggactggg
     3901 ccggggctac ctgggtcggc cggacctgac ggcggagcgg ttcgtgccgg atccattcgg
     3961 gagcgaagcc ggcggccggc tgtaccggac gggtgacctg gcgcggcatc tgcccgaagg
     4021 cgagatcgag ttcctgggcc ggctcgactt ccaggtcaag atccgcggct tccgcatcga
     4081 gctgggagag atcgaggagg cgctgctggc ccacgatcag atccgcgagg cggtggtgct
     4141 ggtcaggccc gttgcaggga gcggcgaggg gagccaggag cgccggctgg tggcctacgt
     4201 ggtgtcggag accggggagg agccggccga cctgcgcggc ttcctgggcc agaggctgcc
     4261 ggactacatg gtgcctccgg tcgtggtcgt gctgccggcg ctgccgctat cggccaacgg
     4321 caagatcgac cgcgcggcgc tgccggctcc ggagatggcc aacgccgtgg cggagcacgt
     4381 gcccccccgc acggccctgg agcgcttcct ggcctccggg ttccgcgacg tgctggcgct
     4441 gccgccggag cgtccggtgg gcctgcacga cgacttcttt gccctgggcg gccactccat
     4501 caccggcgcc atcttcatca accggctgca ggagaccctg tcggaaatcg tgcacgtggt
     4561 gaccctgttc gatcacccga cggtggcgtc gctggccgcc tacgtgacag ggcagcaccc
     4621 acaggccgcg cggcggctct ggggcgagcc ggcgactgtc gagagcgacg tggtggccca
     4681 ggtgggcccc gccgagatcg aggagatgcg ccggctggtg gtcaccggcc ggcccgaggt
     4741 gccggaagag gtgcaggcgc cggcgccgcc caacccgccg gccgtgttcg tgctgtcgcc
     4801 gccgcgctcg gggtcgaccc tgctgcgggt catgctgggc ggccacccgg gcctgttcgc
     4861 gccgccggag ctggagctgc tgtcgttccc caccctgtcg cagcggcgcc aggccttcca
     4921 ggggcgcgac agcttctggc tcgaaggggt ggtgcgggcg gtgatggaag cccggggctg
     4981 cggcgccgag gaggccgagc ggctcatagc cggctacgag gacggcggct ggtccacccg
     5041 gcgcttctac ggcgagctgc agagctggct cggcgagcgc ctgctggtgg acaagacccc
     5101 gtcctatgcg ttggacccgg aggtgctggc ccgggccgag tcctggttca cggccccgcg
     5161 ctacatccat ctgatgcgcg acccgcgggc gaccaacctg tcattcgagg aggccaagct
     5221 ggaccagatt ttcttccgcc ggccgcaccg tttcacgcgg caccaattgg cggaactgtt
     5281 ctggacggtg tgccatcgca acatactgga tttcctgtcg gcgctgccgc gggagcgctg
     5341 gctgccggtg cggttcgagg agctggtgcg cgagccggag cgggtgctgt cgggcatctg
     5401 cgacttcctg gggatcgact accgtccgga gatggcggat ccctaccgcg agggacgccg
     5461 taaggggcgt cgctcacgca tgaccgacgg cgtgcacgac gtatcgcgca tgctggggga
     5521 cgtgaagttc cacacccact cgcgggtgga ctcgtcggcg gcggagcggt ggcggcaggc
     5581 ggaggaggtg ccgctgggca cgccggcacg ggagctagcc ctggagctcg gctacgcgga
     5641 cgcggtgccg gagctggtgg cgcagaccga catcctggtc cggctccagg cgggcgcgcc
     5701 ggaggtccgg ccgctgttct gcgtgcatcc ggtgggcggc gacgtggctt gctaccgcga
     5761 gctggcgcgg cgcctgggcc cggatcagcc cgtctttggt ctccgctcgc cgcaggagcc
     5821 gatccctgat ctgcggcaga tggccacgct ctacgtcgag gtggcgcgcc aggcgcagcc
     5881 ggcggggccc taccggctgg cgggttggtc catgggcggt ctggtggcct acgagatggc
     5941 ccgccagctc gaggagcagg gagaggcggt ggagctggtg gccttgatcg atgccgcttc
     6001 gccgcagtgg tgggcggagc agacgccctc gagcgacggg gagctggtgg cctggttcgc
     6061 gcgggatctg gcgcggctgt tcggggcggc gcaggtgccg gcggtgcgcc tcgacgcccc
     6121 ggacgtcgat ggcaacctgg cgcgggtgct ggaggcgggg cacgcgaccg gcctggtagc
     6181 cgcgggcgtg gggctcgccg acctgcgtcg gctgttcgag gtgttccgcg ccaaccgcat
     6241 cgccctctcc tcctatcctc cgctccccta ctccggcgcg ctggagctga tccgcgccgc
     6301 cgagacaggc ggtgaagagg gagaggaccc ttaccggggc tggagcgggc tggccgccgg
     6361 aggggtcacc gttcacacag tgcagggaga tcactactcg attgtgctgg gagagggggt
     6421 ggagcggctg accgggctcc tgcgctcctg gctctccggc ccgaaggacg gagagctgtc
     6481 gcatggctga gactgtagat cgtctggcag gcctgtcgcg ggagaggcgg gcgctgctgg
     6541 agtccttgca gccgagccgg gaatcgttct gacgagagac gaggagaaat caatgtacaa
     6601 gatcagcctg atcaatatgc cctttgccaa cctgggcttg ccatcaatcg cactgaccca
     6661 gctcaaatcg atagccgagc agcgattcgg gggggatgtc cgggtccgca tcttgtatct
     6721 caacctgaat ttctcccagt acctggggac cgacgtctac ggccacatca ccggggcgct
     6781 ccaggcgaac aactccgggc tgggagactg gctcttccga cagatcgcct ttcccaatca
     6841 gccggacaac accgcctctt attttcagcg ctacttcccc cggatggatg ctgagacgag
     6901 cgtcgtcaag gggtcgatcc tcgccaagcg ggccgggctc aagcgctact tcgagcgcct
     6961 cgtgacccag taccagctcg accgcgagga cctggtgggc ttctcctcca tgttcgccca
     7021 aaacgtcgcc tccatggcca tggctcgagt gatcaaagac cgcaatgcgg cggtcatcac
     7081 ggtgatgggc ggagcgaatt gcgagagccc catgggtgag gagctggtgc gccatgtcga
     7141 ggcgctcgac tttgtcttct cggggccggc gctcatcagc ttccccgatc tcgtcgcggc
     7201 ccagcttgcg ggcgaccgcg accggtgcca ccagatccgt ggcgtgttct cccgccaaaa
     7261 cgtcggcgcg ccgcacatgc aggggcatgg cgccatgggc gccgagttgc cgatcgagac
     7321 cccggtcccc ctggactacg aatcctttct cgacgacctg agccgcagct tccccgacgg
     7381 tagcgtccag ccgtccctga cgttcgagac ctcccgcggc tgctggtggg gggagcgctc
     7441 ccactgcacg ttctgcggtc tcaacggcgg caccatgatg tatcgggcca tgccgtcgga
     7501 tcaggcgttg gcgctgttcc gggagatttt cgagaagtat ggcgaccggt gcacgaacat
     7561 cttctcggta gacaacatca tgccacggca gtacatcaag gaggtgttcg cgcacctcgt
     7621 gccgccagag gggaccagca tcttctacga ggtcaaggcg gacttgaagg agtgggagat
     7681 ggaggtcctg agccgcgcgg gcgtgaccga gattcagccc gggatcgagg ccctggccac
     7741 ctccactctg aagctgatgg ggaaggggac cacgtcgttc cagaacgtcg cttttctcaa
     7801 gagctgcctg cgctacggta tccatcccgc ctggaacctg ctcatcggtt ttccgcgcga
     7861 agaggagacg gtctacgaag gatatctgcg cgccctgccc ctgctgatgc acctgccgcc
     7921 gccggccggc gcgttcccga tccgcttcga caggtacagc ccgtactaca cccgcgccgc
     7981 ggaatacggc cttgacctgg cgccgtacgc gttctatggc gccatctatc cattcggcga
     8041 ggaggcgctg agccggatgg cctactactt cgaagatcgc cactacgacg cggactacct
     8101 cgtccgtctg ctccggtggc aggagcggct caacgacacg gtgcagcgct gggtgcagcg
     8161 ctgggagggg cgcgacggcg ctctccgggc ggagctccgg ctgaagatgg acggcgatgg
     8221 cgccgtggtt cacgacaccc gcaccggcga gcgggtcgag catcggctga gcgcgctcgg
     8281 gctcgacgta ctccatgccg cgcgcggacg cgggctcggc gcgcgtcacc tgcgggacca
     8341 gatgggcatc gacgagccga ccgccgtggc agagctcgcc cgactcgcgg agctcggtct
     8401 ggtcttcgag gaggacgggc gcttcatcgg cctcgtgatg gacatcgagg ccagggcgcc
     8461 ggacctcgcg ctccagggca ttacgagcgt ggcggaggac gaacggttgg cgtcggccgc
     8521 gggaggagat cgacgtgagg tgcaaccgtg aatggccagg tgccgaagaa gtttgtc
//
LOCUS       DASPJI010000899         4999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_512919, whole genome shotgun sequence.
ACCESSION   DASPJI010000899 DASPJI010000000
VERSION     DASPJI010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512919"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4999)
                     /locus_tag="VEL74_25520"
     CDS             complement(<1..>4999)
                     /locus_tag="VEL74_25520"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HYG65959.1"
                     /translation="LAALEPWFRRHGDESPRLVNMYGITETTVHVTYRPLSWADLEAA
                     PRSPIGVAIPDLTLHLLGSGGEPLPVGVAGEIHVGGAGLARGYLGRPELTAERFVPDP
                     FAAWRGQPGARLYRSGDLARRLADGGLEFLGRLDHQVKIRGFRIELGEIEVALVASPG
                     VRDAVVLVRPDRLGEMALTAYVVPAGEGATDAGALRTHLRSHLPEHMVPAGFVLLDAL
                     PLTANGKLDRKALLRLAPEADGTAGSGRAARRFVAPRTPAEEVVATIFAEVLRLDRVG
                     VHDGFFDLGGHSLLATQVMARLRETFAVSLPLRGLFEAPTVAALAERVERARGGGITE
                     GVVEAPPIVPVPRPHGEDGGLPLSFAQQRLWFLDRFEPGGVAYNVPLAMRLTGPLDAG
                     TLARCLAEVVRRHEVLRTTFPERGGSPVQEIAAELPLPLPVIELGRLPEREREAEVLR
                     LAVAQARQPFDLATGPLLRWALLRLAPEEHVMLAAAHHIVCDAWSMGVLAGELGALYA
                     AFSRGETSPLPALPVQYADFAAWQRRWLAGARLEWELAYWRQRLAGAPQALDLPLDRP
                     RGAVRGRRGESVPVLLSPSLNAALNELARSEGATLFMTLLSAFQALLGRWSGQDDLLV
                     GTPVAGRGRRELEGLIGFFVNTLVLRADLAGAPSFRELLQRARTAALDAFAHQDLPFE
                     RLVEAVAPERDLSRSPLFQVMFALQNVPVEGIAMPGLTLSPIELDPGVAKFELTLALR
                     EEEGGLVGSLEYDADLFDGSTAALLLSHLTRFLEAVAADPGQKIDAVPLLVPAERQQL
                     LANLAGGLARRTPRDTVAPAARLFVAPRTPAEEVVATIFAEVLRLERVGVHDRFFDLG
                     GHSLLAMQVTSRLRDTFAVELPLRDLFEAPTVAALAERVERARGGGGTEGVVAAPPIV
                     PVPRPHGEDSALPLSFAQQRLWFLDRFEPGGFAYNVPLAMRLAGPLDAGTLARCLDEV
                     VRRHEVLRTTFAERGGSPVQVIAAELALPLPVIDLGRLPEREREAEVRRLAVAQARQP
                     FDLAAGPLLRWALLRLASEEHVMLATAHHIVSDAWSMGVLAGELGALYAAFSRGEASP
                     LPALPVQYADFAAWQRRWLAGARLEWELAYWRQRLAGAPRALDLPLDRPRGEVRGRHG
                     ESVPVLLSPSLTAALTELARSEGATLFMTLLSAFQALLGRWSGQDDVLVGTPVAGRSR
                     RELEGLIGFFVNTLVLRADLAGAPPFRELLQRARVTALDAFAHQELPFEQLVEAVAPE
                     RDLSRSPLFQVMFALQNVPVERIAIPGLTLSPIPLEPGVAKFELTLALREEEGGLAGS
                     LEYDADLFDGSTAALLLTHLTGFLEAVAADPGQKIDEVPLLVPAERQQLLANLAGGLA
                     RRTPRDTAAPAAARPFVAPRTPAEEIVAAVFAEVLRLERVGADDRFFDLGGHSLLAMQ
                     VMSRLRETLAVELPVRDLFEAPAVSALAERVERARAARRAAPVVPVPRPQGEDSGLPL
                     SFSQQRLWFLERLAPGGVAYNVPLSTRMSGPLDAQALERALAEVVRRHEVLRTTFAER
                     DGSPVQVIASELRVPLPLIDLGALPEPAREAEAVRLATVQARRPFDLATGPLVRWVLL
                     RLETEEHVVLAAAHHIVSDAWSMGILVGELGALYTAFSRGEGSPLPPLPVQYADFA"
BASE COUNT          762 a         1964 c         1743 g          530 t
ORIGIN      
        1 cggcgaagtc ggcgtactgc acggggagcg gcggcagcgg cgagccttcg ccgcgggaga
       61 aggcggtgta gagcgctccc agctcgccga ccaggatgcc catggaccag gcgtcggaga
      121 cgatgtgatg ggcggcggcg agcaccacgt gctcttcggt ctccagccgc agcagcaccc
      181 agcggaccag agggcccgtg gccaggtcga acggccgccg cgcctggacc gtcgccaggc
      241 ggaccgcctc ggcctcgcgt gccggctccg gcagggcccc gaggtcgatc agcggcagcg
      301 gcacccgcag ctcggacgcg atcacctgga ccgggctgcc gtcccgctcc gcgaacgtcg
      361 tccgcagcac ctcgtgccgg cgcaccacct ccgccagcgc ccgctccagc gcctgggcgt
      421 cgagagggcc gctcatccgg gtggagagcg gcacgttgta ggcgacgccg cccggcgcga
      481 gccgttccag gaaccagagg cgttgctgcg agaaggacag cggcaggccg ctgtcttctc
      541 cctgggggcg cggcaccggc acgaccggcg cggcacggcg ggcggcgcgc gcccgctcga
      601 cgcgctccgc cagcgccgac acggccggcg cctcgaacag gtcgcggacc ggcagctcca
      661 ccgcgagcgt ctcgcgcagg cgcgacatca cctgcatcgc cagcagcgag tggcctccca
      721 gatcgaagaa gcggtcgtcg gcccccaccc gctcgagccg caggacctcc gcgaagaccg
      781 ccgccacgat ctcctcggcc ggggtgcgcg gcgcgacgaa cggcctcgcg gcggcggggg
      841 ccgcggtgtc ccgaggggtc cgccgcgcca ggccacccgc cagattcgcc agcagttgct
      901 gccgctcggc aggcaccagc agggggacct cgtcgatctt ctgtccggga tcggcggcca
      961 ccgcttcgag gaagccggtg agatgagtaa ggagaagagc cgccgtggag ccgtcgaaca
     1021 ggtccgcgtc gtactccagg ctccccgcca gcccgccctc ctcctcccgc agggccagcg
     1081 tcagctcgaa cttcgccacc cccggttcga gcggaatcgg cgacagggtc agccccggta
     1141 tcgcgatccg ctcgaccggg acgttctgca gagcgaacat cacctggaac agcggggaac
     1201 gggagaggtc ccgctccggc gccaccgctt cgaccagttg ctcgaacggc agctcctggt
     1261 gcgcgaaggc gtccagcgcc gtgacacggg ccctctgcag gagctcgcgg aacggtggcg
     1321 ccccggcgag gtccgcccgc agcaccaggg tattgacgaa gaacccgatc agcccctcga
     1381 gctcccgccg gctgcggccc gccaccggcg tcccgaccag cacgtcatcc tggccggacc
     1441 agcgcccgag cagcgcctgg aaggccgaca gcagggtcat gaatagcgtc gccccctcgc
     1501 tccgggcgag ctcggtcagg gcggcggtca gggaggggga gagcagcacc gggaccgact
     1561 ctccgtggcg gccgcgcacc tccccccgtg gccggtccag cggcagatcc agcgcccgcg
     1621 gcgcgccggc gaggcgctgg cgccagtacg ccagctccca ctccagcctg gcgccggcga
     1681 gccagcggcg ctgccacgcg gcgaagtcgg cgtactgcac cggcaatgcc ggcagcggcg
     1741 acgcctcgcc ccgggagaac gccgcgtaca gcgcgcccag ctcgccggcc agcacgccca
     1801 tggaccaggc gtcggagacg atgtgatggg cggtggccag catcacgtgc tcctccgaag
     1861 cgagccgcag cagcgcccag cggagcagcg ggcccgcggc cagatcgaac ggctgccgcg
     1921 cctgcgccac ggcgagccgg cgcacctcgg cctcgcgctc gcgctccggc agcctgccca
     1981 ggtcgatcac cggcagcggc aacgccagct cggccgcgat cacctgcacg gggctgcccc
     2041 cccgctccgc gaaggtggtc cgcagcacct cgtgccggcg cacgacttcg tcgaggcacc
     2101 gcgccagtgt tccggcgtcg aggggcccgg ccagccgcat cgccagcggc acgttgtagg
     2161 cgaagccgcc aggctcgaag cggtcgagga accagaggcg ctgctgggcg aaggacagcg
     2221 gcagggcgct gtcttctcca tgggggcgcg gcaccggcac gatcggcggc gccgcgacga
     2281 cgccctccgt gccgccgccg ccgcgggccc gctccacgcg ctccgcaagg gccgccacgg
     2341 tcggcgcctc gaacaggtcc cgcagcggca gctccaccgc gaaggtatcc cgcagccgcg
     2401 acgtcacctg catcgccagc agcgaatggc cgcccaggtc gaagaagcgg tcgtgaaccc
     2461 ccacccgttc gagccgcagc acctcggcga agatcgtcgc cacgacctcc tcggccggcg
     2521 tgcgcggcgc gacgaacagc ctggcggcgg gggccacggt gtcccgaggg gtccgccgcg
     2581 ccaggccacc cgccagattc gccagcagtt gctgccgctc ggcaggcacc agcaggggga
     2641 ccgcgtcgat cttctgtccg ggatcggcgg caaccgcttc gaggaagcgg gtgagatggg
     2701 agaggagaag agccgccgtg gagccgtcga acaggtccgc gtcgtactcc aggctcccca
     2761 ccagcccgcc ctcctcctcc cgcagggcca gcgtcagctc gaacttcgcc actcccggat
     2821 cgagctcgat cggcgacagg gtcagccccg gcatcgcgat cccttcgacc gggacgttct
     2881 gcagcgcgaa catcacctgg aacagcgggg aacgggagag gtcccgctcc ggcgccaccg
     2941 cctcgaccag ccgctcgaac ggcaggtcct ggtgcgcgaa ggcgtccagg gcggcggtcc
     3001 gggccctctg caggagctca cggaacgagg gcgccccggc gaggtccgcc cgcagcacca
     3061 gggtattgac gaagaacccg atcagcccct cgagctcccg ccggccgcgg cccgccaccg
     3121 gcgtccccac cagcaggtcg tcctgaccgg accagcgccc gagcagcgcc tggaaggcgg
     3181 acagcagggt catgaacagc gtcgccccct cgctccgggc gagctcgttc agggcggcgt
     3241 tcagggaggg ggagagcagc accgggaccg actctccgcg gcggccgcgc accgcccccc
     3301 gcggccggtc aagcggcaga tccagcgcct gcggcgctcc ggcgaggcgt tggcgccagt
     3361 acgccagctc ccactccagt ctggcgccgg cgagccagcg gcgctgccac gcggcgaagt
     3421 cggcgtactg caccggtaat gccggcagcg gcgacgtctc gccccgggag aacgccgcgt
     3481 acagcgcccc cagctcgcca gccagcaccc ccatggacca ggcatcgcag acgatatgat
     3541 gggcggcggc cagcatcacg tgctcctccg gagcgagccg cagcagagcc cagcggagca
     3601 ggggacccgt ggccagatcg aagggttgcc gcgcctgcgc gacggccagc cggagcacct
     3661 cggcctcgcg ctcgcgctcc ggcagccttc cgagctcgat caccggcagc ggcagcggca
     3721 gctcggccgc gatctcctgg acggggctgc caccccgctc cgggaacgta gtccgcagca
     3781 cctcgtgccg gcgcaccacc tccgccaggc accgcgccag cgttccggcg tcgagaggcc
     3841 cggtcagccg catcgccagc ggcacgttgt aggcgacgcc acccggctcg aagcgatcga
     3901 ggaaccagag acgctgctgc gcgaacgaca ggggcaggcc gccgtcttct ccatgggggc
     3961 gcggcaccgg cacgatcggc ggcgcctcga cgacgccctc cgtgatgccg ccgccgcgag
     4021 cccgctcgac gcgctccgcc agggccgcaa cggtcggcgc ctcgaacagg ccccgcaacg
     4081 gcagcgacac cgcgaaggtc tcccgtagcc gcgccatcac ctgcgtcgcc agcagcgaat
     4141 ggccgcccag atcgaagaag ccgtcgtgga cccccacccg gtcgagccgc agcacctcgg
     4201 cgaagatcgt ggccacgacc tcctcggccg gcgtgcgcgg cgcgacgaac cgccgggccg
     4261 ccctgccgga accggccgtg ccgtccgcct ccggcgccag ccgcagcaga gccttgcggt
     4321 ccagcttgcc gttggcggtc aacggcagcg cgtccaggag cacgaagccc gccggcacca
     4381 tgtgctccgg caggtggctc cgcaggtgcg tccgcagggc acccgcgtcg gtagcgccct
     4441 ccccggcggg caccacgtag gccgtcaggg ccatttcgcc cagccggtcc ggtcggacca
     4501 gcaccaccgc gtcgcgcacc cccggcgacg ccaccagcgc cacctcgatc tcccccagct
     4561 cgatgcggaa gccgcggatc ttgacctggt ggtcgagacg ccccaggaac tcgaggccgc
     4621 cgtcggccag gcgccgcgcc aggtcccccg accggtacag ccgcgccccc ggctgccccc
     4681 tccaggcagc gaacggatcg ggcacgaacc gctccgccgt cagctccggc cggcccagat
     4741 agccgcgcgc cagaccggcg ccgccgacgt gaatctcccc cgccaccccg accggcagcg
     4801 gctcgccacc ggagcccagc agatgcagcg tcaggtccgg aatggcgacg ccgatgggac
     4861 tgcgcggcgc cgcctccagg tccgcccacg acagcggacg gtaggtgacg tgcaccgtcg
     4921 tctcggtgat gccgtacatg ttgaccagcc gcggcgactc gtccccatga cgccggaacc
     4981 agggctccag cgccgccag
//
LOCUS       DASPJI010000900         5008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_285489, whole genome shotgun sequence.
ACCESSION   DASPJI010000900 DASPJI010000000
VERSION     DASPJI010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285489"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VEL74_25525"
     CDS             <1..1023
                     /locus_tag="VEL74_25525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HYG65960.1"
                     /translation="AVSAPSGASAAEGGLRCEEVLFPVTVAPGATEVYNVFGVLCSRG
                     SINNKTVQITLHGSTYSHLYWDWPFQPETYSYMRRATAAGYAVLNIDRIGIGQSDHPP
                     AEQVTIGADAFVVHQIVQALRGGELVVPSFGRIEAERVALVGHSLGSVIAIQEAATYG
                     DVDGVALTGVSHTVTPVLNDILGGLYPASLDPRFAGRNIPDGYLTSLPGTRGIFYHLP
                     AADPLVLAIDEQTKETVTPAELATAFPALDMSTGVHTPVLAIIGDLDLAFCNAPSCSA
                     SGSFANEASFYPADACAETVIMPGVGHDLNLHYAAQDSYDAILDWLDRRVGSDTRLPV
                     PVPCQP"
     gene            2133..2762
                     /locus_tag="VEL74_25530"
     CDS             2133..2762
                     /locus_tag="VEL74_25530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014349964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HYG65961.1"
                     /translation="MTKPKFGQDFWEQLWSKTLREDADKVARRPPNAHLMAEAASLPP
                     GRALDAGCGHGADTLWLAAQGWQVTAVDFSAAALAHGRSMAEAAGADIAERIDWIESD
                     LARWTPESGHYDLVVCLYVHVAGSVEDMVGRMANGVAPGGTLFLVGHRPIDPSTGAAT
                     AAASQVQVSVEAAVAALDPNVWELIVAEERPRAAAGTGVDAVIRARRRT"
     gene            complement(2938..3231)
                     /locus_tag="VEL74_25535"
     CDS             complement(2938..3231)
                     /locus_tag="VEL74_25535"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYG65962.1"
                     /translation="MMDDDVNELQSLLRDAAALLRASGAEAVYVFGSVATGKNRDTSD
                     VDLAVSGLPPGSFFETMARLGDLLDRPVDLVDLDVSSPFTAYLKEKGLLRRVA"
     gene            complement(3334..>5008)
                     /locus_tag="VEL74_25540"
     CDS             complement(3334..>5008)
                     /locus_tag="VEL74_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010988636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HYG65963.1"
                     /translation="EYRKHIEKDAALERRFQPIIIDQPGVEDTISILRGLRERFEVHH
                     GVRIQDNALVAASVLSHRYITDRFLPDKAIDLIDEACAMIRTEIDSMPQELDAITRRV
                     MQLEIEEAALKKEKDKASQERLEDLRRELADLKAQADSMRAQWEAEKSSIEEVRGLRE
                     QIEQVRQEIERAERAYDLNKSAELRYGKLPQLEGQLKEREARLAEKGGQLVREEVTEE
                     EVAEIVSRWTGIPVTRLVEGEREKLLRMEDILHDRVVGQDEAVKLVADAVIRARAGIK
                     DPRRPIGSFIFLGPTGVGKTELAKTLAQTLFDSEDNIVRIDMSEYMERHNVSRLIGAP
                     PGYVGYEEGGQLTEAVRRKPYSVILFDEIEKAHNDVFNVLLQILDDGRVTDSQGRTVD
                     FKNTVIIMTSNIGSHHLLEGVTREGEITEAARGAVMRELRSHFRPEFLNRVDDIVLFR
                     PLTLSEITRIVDLQTDDLRRRLTDRGVRLELTEAAKEYVARQGFDPVYGARPLKRYLQ
                     HELESRIGRALIAGQVTEGSLVRVDVQGGQLVVAIEAPKEAEAEVPVAV"
BASE COUNT          794 a         1678 c         1667 g          869 t
ORIGIN      
        1 gccgtgtccg ctccgtccgg cgcctcggcg gcggaggggg gcctgcgctg tgaagaggtc
       61 ctgttccccg tgaccgtggc gccgggtgcc accgaggtct acaacgtctt cggtgtcctc
      121 tgctcgcgcg gcagcatcaa caacaagacg gtccagatca cgctccacgg ctcgacctac
      181 agccatctct actgggattg gccgttccag cccgagacct actcctacat gcgccgcgcc
      241 acggcggcgg gctacgccgt cctcaacatc gaccggatcg gcatcggcca gagcgaccat
      301 ccgccggcgg agcaggtgac catcggcgcc gatgcgttcg tggtccatca gatcgtccag
      361 gcgctgcgcg gcggcgagtt ggtggtcccc tcgttcggcc ggatcgaggc ggagcgggtg
      421 gcgctggtcg ggcactcgct cggctcggtg atcgcgatcc aggaggcggc cacctatggc
      481 gacgtggacg gcgtggcgct gacgggcgtc tcccacacgg tcaccccggt gctcaacgac
      541 atcctgggcg gcctctatcc ggcgagcctc gacccgcgct tcgcgggccg gaacatcccg
      601 gacggctatc tcaccagcct gccgggtacg cgcggcatct tctaccacct gccggccgct
      661 gatcccctgg tgctcgccat cgacgagcag accaaggaga ccgtcacccc ggccgagctg
      721 gcgacggcct tcccggcgct cgacatgtcg accggcgtcc acactccggt gctggcgatc
      781 atcggcgacc tcgacctcgc gttctgcaac gcgccgtcct gctcggcctc cggcagcttc
      841 gccaacgagg ccagcttcta cccggcggac gcctgcgccg agacggtgat catgcccggc
      901 gtagggcacg acctgaacct gcactacgcg gcccaggact cctacgacgc cattctcgac
      961 tggctggacc gccgcgtcgg cagcgacacg cggctgccgg tgccggtccc ctgccagccc
     1021 tgacccccac ctatccgcgc acggctcccc tcacccgcac gccgtgcgcg gatcgtgcac
     1081 atcgattgtc aaagaacacg gactcaccgc tacagagtcc gtccttcgag cagggacact
     1141 accacaaagg cctgcttgtt cgactgaatt gacatctctg ccgagagctc tgtagtctcc
     1201 ctcccgctgc gctgccgtgg agacacagag agcgaagtca gggaggaggc attgattcct
     1261 caactcttcg ccgccgttcc gtactactga ggggatctcc ctcagtgtca aggagctatc
     1321 cctcgataga gagggatggc tcctcaatgt tgaggaacgg ccgggacgtg ttgaggaacc
     1381 gcggcgaagc actgaggagc cggcctggaa cacttcgggg tcatctctca gtgttgaggg
     1441 gcggcggcgt agcgttgagg gagagctcct cgaaattgag gagccgtccc tcagctctga
     1501 ggagcgacgc cggaccactg agggccggcg gcgaagcagt gaggggacgg cccggaacac
     1561 tccgggatga tccctcagtg ctgaggagcc gcggcgaagc gttgaggagc ggcggagaag
     1621 cgttgaggaa cgccgcctca atactgagga gtcgtcccga aacggctccg ggttcattga
     1681 caactgaatg gtgacggggc gccgccaggt ccatggccgc gctcctgagc attcctccgt
     1741 ccgacaggag gaccgccaga aaggcgccac cggcaggcgt ggccggtggc gtctctctcg
     1801 cgcggtagct ataagagcct ctaacaagac aggacttctt catggagggg aggggcttgt
     1861 cccctcccgg gaggggaccg aaaagccaga cgcattgagg agcggccccg aagtgttgag
     1921 ggatggtgcc ttcaacaccg aggaaccggc ccggaacgcg tcccctaacg ccggcacttg
     1981 tacgcgggcg gctgacttcg ccatccggcg gtgaagcgca gagtgttata cgcttcgcgg
     2041 aaggggcgcg gctcgtctcg tgggctgcgc ctggtgcttg cttgagtagg actcagttcg
     2101 cgacgatacc tccgactcct cgcaatggcc tcatgaccaa gccaaaattc ggtcaggact
     2161 tctgggagca gctatggtcg aagacgctgc gtgaggacgc ggacaaagtc gcgcgccgtc
     2221 cacccaacgc gcatctgatg gccgaggctg ccagccttcc gccggggcgc gccctcgacg
     2281 ccggctgtgg acatggcgcg gacacgctct ggctcgccgc gcagggctgg caggtcacgg
     2341 cggtcgactt ctctgcagcg gcactggctc acggacggtc gatggctgag gctgccgggg
     2401 ccgatatcgc agagcgcatt gactggatcg agagcgatct cgcgagatgg acgccggagt
     2461 cggggcatta cgatctcgtt gtttgcttgt acgtccacgt ggccggttcc gtggaagaca
     2521 tggtggggcg gatggcgaac ggcgtcgcac cgggagggac cctgttcctg gttggacacc
     2581 gcccgatcga tcccagcacc ggagctgcca cggccgcggc aagtcaggtg caagtctccg
     2641 tcgaggctgc ggtcgctgcg ctcgatccca atgtatggga gttgatcgtc gcggaggagc
     2701 gtccgcgcgc ggctgccggc acgggtgtgg atgcggtgat ccgcgcgcgc cgccggacct
     2761 gaatcaccgc ggcgtggtgg ccgtctagtt ccgttgtaaa aggagtggag aaaagcgccc
     2821 agagctgata gctcgattgc gttgggttcc cggacctggc actgagcgag cagtggccgg
     2881 tgcatctcca ggagcttatg gagttgagcc tcctcgactt cgatctggtc cttgagctca
     2941 ggccacacgc cggaggagcc ccttctcctt gagataagct gtgaaggggc tggatacgtc
     3001 caggtcgacg aggtccaccg gcctgtccag caggtcaccc agacgggcca tcgtctcgaa
     3061 gaaggagcct ggcggcagac ctgataccgc gagatcgacg tccgaagtgt cccggttctt
     3121 gccggtagcg accgaaccaa agacgtagac cgcctcggcc cccgatgccc tgaggagagc
     3181 cgcggcgtcc cgcaagaggc tctgcaactc gttcacgtcg tcgtccatca tgaggatagt
     3241 ctggcctcca ggggtgggtg tctgtcaagg ccagaagaga aacaagcccg gcctcgcggc
     3301 cgggcttgga agattggtag aggaggggag gccttacacc gccaccggca cctcggcctc
     3361 tgcctctttc ggagcctcga tcgccaccac gagctggccg ccctgcacgt ccaccctcac
     3421 cagcgagccc tcggtgacct ggccggcgat cagggcgcgg ccgatgcggc tctccaactc
     3481 gtgctggagg tagcgcttga ggggccgggc gccgtagacc gggtcgaagc cctgcctggc
     3541 cacgtactcc ttggcggcct cggtcagctc caggcgcacg ccacggtcgg tcagacggcg
     3601 gcgcaggtcg tcggtctgga ggtcgacgat gcgcgtgatc tcggagagcg tcagcggtcg
     3661 gaagagcacg atgtcgtcca cccggttcag gaactccggc cggaaatggg agcggagctc
     3721 cctcatcacg gcgcccctcg ccgcctcggt gatctctccc tcccgcgtca caccctccag
     3781 cagatggtgc gagccgatgt tcgaggtcat gatgatcacg gtgttcttga agtccaccgt
     3841 gcgcccctgg ctgtcggtca cccggccgtc gtcgaggatc tggaggagca cgttgaacac
     3901 gtcgttgtgc gccttctcga tctcgtcgaa caggatcacc gagtagggct tccgccgcac
     3961 cgcctccgtg agctggcccc cctcctcgta gccgacgtag cccggaggag cgccgatcag
     4021 gcgcgacacg ttgtgccgct ccatgtactc gctcatgtcg atgcggacga tgttgtcctc
     4081 gctgtcgaag agcgtctggg cgagcgtctt cgccagctcg gtcttgccga cgccggtcgg
     4141 gcccaggaag atgaacgagc cgatggggcg ccgcgggtcc ttgatgccgg cccgggcgcg
     4201 gatgaccgcg tccgccacca gcttcacggc ctcgtcctga ccgaccaccc ggtcgtgcag
     4261 gatgtcctcc atgcgcagga gcttctcccg ctcgccctcg accagacggg tcaccgggat
     4321 gccggtccag cgcgatacga tctcggcgac ctcctcttcg gtcacctcct cgcgcacgag
     4381 ctggccaccc ttctcggcca gccgcgcctc ccgctccttg agttggccct cgagctgcgg
     4441 cagcttgccg tagcgcagct cggcgctctt gttgaggtcg taggcccgct cggcccgctc
     4501 gatctcctgg cgcacctgct cgatctgctc gcgcaggccc cgcacctcct cgatcgagct
     4561 cttctcggcc tcccactgcg cccgcatgga gtcggcctgg gccttgaggt cggccagctc
     4621 gcggcggagg tcctccagcc gctcctggct cgccttgtcc ttctccttct tgagcgccgc
     4681 ctcctcgatc tcgagctgca tgacccggcg ggtgatcgcg tccagctcct gcggcatgga
     4741 gtcgatctcg gtgcggatca tggcgcaggc ctcgtcgatg aggtcgatcg ccttgtccgg
     4801 caggaagcgg tccgtgatgt agcggtgcga gaggaccgac gcggccacca gggcgttgtc
     4861 ctggatgcgc acaccgtggt gcacctcgaa gcgctcgcgc aggccgcgga ggatcgagat
     4921 ggtgtcctcg acgcccggct ggtcgatgat gatgggctgg aagcggcgct cgagcgcggc
     4981 gtccttctcg atgtgcttgc ggtactcg
//
LOCUS       DASPJI010000901         3661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_513452, whole genome shotgun sequence.
ACCESSION   DASPJI010000901 DASPJI010000000
VERSION     DASPJI010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513452"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3661
                     /locus_tag="VEL74_25545"
     CDS             <1..>3661
                     /locus_tag="VEL74_25545"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HYG65964.1"
                     /translation="GEGERRLVGYVVGGAGRDLDLTALRAHLQARLPEHMVPLLMALP
                     ALPLTVNGKVDRRALLEPEAQRPRGDRRRPETATEEVLAAIWSRVLELAEVGTEEAFF
                     ALGGDSILSLQVVALAKERGLALDLQDLFRHQTIRALAGAIEGKTAAAAPVHSAPFSL
                     VAPEDRALLPDGLEDAYPLATLQAGMLYHMELTPEDPLYHNVDSWHMRGRFEPETFAE
                     AMRRVIARHPVLRTSFALSGYSEALQLVHRRVELPLVIEDIRHLDPEAQEAAVDALMA
                     REKRRGFDLAGAPQIRFHVFLRSADTFQFSLTENHALFDGWSLHSTLAEIFELYFVLL
                     KEGAVPPERTAPLSLTYREFVRREREALASAAAAAYWQGVLAELEPLELPRWPEPWRS
                     AGPRRVGMLDVPAGPGVTEGLERLARRATVPLKSVLLAAHCKVLSRLAGRPEVVTGLV
                     SNGRLEESEGDQVRGLFLNTLPLRLRLPGGSWEDLVRAVFQAEQEMLPHRRYPFGALQ
                     RQWGQRPLYEVAFNYIHFHVIDALAASGNLEALGFKRVEPTNLGLQVHFGRSSGSGLM
                     VQLDYDTQRFAAAQMALVGGYYGRTLAAMAAGLDTPLLSPAERQALLAESNDTRVERP
                     RGLLLHQLVAPGAVVSQGSLVLSQDELEARSNRLARHLLSLGVSPETRVGISLERTPE
                     LVVALLAVLKAGGAYVPLDPSHPAERTALILDDAGVALLLTGESLERDREAIAARSGA
                     PLPPLATEDNLAYVIYTSGSTGRPKGVQLSHRAVVNFLLSMAERPGLTAADVVLALTT
                     LTFDIAGLEIYLPLLVGGRVEMVTREEATDGVRLAARLAACGATAVQATPATWRMLLE
                     SGWQGDPRLKALCGGEALPRELAEALRARVGSLWNVYGPTETAVWSAAGEVTEGTGPV
                     PVGPPIANTGLYVVAAETLELVPVGVAGELWIGGAGLARGYLGRPDLTAERFVPDPFA
                     AASGEAGARAYRTGDLVRRLPDPAGGLEFLGRADFQVKVRGYRIELGEIEAALARHPA
                     VAQAVAVARADGGETLLAAYLVAREGEAVPGPAELRAFLLRGLPEYMVPAAFVALPVF
                     PLTPSGKIDRRALSGRDAGPDAVRQGERYVAPEGPVEELLAQIWAEVLRAGRVGAEDG
                     FFELGGHSLLATQVISRIRASLGVELPLRRLFEAGTVRGLARAVEAARQESGEAPLPP
                     IARVPRDGALPLSFAQ"
BASE COUNT          428 a         1293 c         1433 g          507 t
ORIGIN      
        1 gggcgaaggg gagcggcggc tggtgggcta cgtggtggga ggcgccggcc gggacctcga
       61 cctcacggcc ctgcgcgcgc atctccaggc gcggctgccg gagcacatgg tgccgctgct
      121 catggcgctg ccggccctgc cgctcaccgt caacggcaag gtggaccgcc gcgcgctcct
      181 cgagcccgaa gcccagcgcc cgcgggggga tcggcggcgg cccgagaccg ccaccgagga
      241 ggtgctggcg gcgatctggt cgcgggtgct ggagctcgcg gaggtgggga ccgaggaggc
      301 cttcttcgcc ctgggcggcg actccatcct cagcctccag gtggtggcgc tggcaaagga
      361 gcgggggctg gccctggacc tccaggacct gttccggcac cagaccatcc gggctctggc
      421 cggcgccatc gaggggaaga cggccgccgc ggcgccggtc cacagcgccc ccttctccct
      481 ggtcgcgccg gaggacaggg cgctgctgcc ggatggattg gaggacgcct accccctggc
      541 caccctccag gccgggatgc tctaccacat ggagctgacg ccggaggacc cgctgtacca
      601 caacgtagac tcctggcaca tgcgcgggcg cttcgagccg gagacgttcg ccgaggcgat
      661 gcggcgggtg atcgcgcgcc accccgtgct gcgcacctcg ttcgccctgt ccggctacag
      721 cgaagcgttg cagcttgtgc accggcgggt ggagctgccg ctggtgatcg aggacatccg
      781 ccatctcgac ccggaggcgc aggaggcggc ggtggacgcg ctgatggcgc gcgagaagcg
      841 gcggggcttc gacctggccg gggcgccaca gatccgcttc cacgtcttcc tgcgctcggc
      901 cgacaccttc cagttctcgc tcaccgagaa ccatgccctc ttcgacggct ggagcctgca
      961 ctcgacgctg gcggagatct tcgagctcta cttcgtgctg ttgaaagagg gggcggtgcc
     1021 cccggagcgg acggcgccgc tctccctcac ctaccgcgag ttcgtacggc gggagcggga
     1081 ggccctcgcc tcggcggcgg cggcggcgta ctggcagggg gtgctggcag agctggagcc
     1141 gctggagctg ccgcgctggc cggagccgtg gcggagcgcc gggccgcgcc gggtagggat
     1201 gctggacgtg cccgccggcc ccggggtgac ggaggggctg gagcgcctgg cgcggcgcgc
     1261 gacggtaccg ctcaagagcg tgctgctggc ggcgcactgc aaggtgctga gccggctggc
     1321 ggggcggccg gaggtggtca ccggcctggt gtcgaacggc cggctggagg agagcgaggg
     1381 ggaccaggtg cggggcctgt tcctcaacac cctgccgttg cggctgcggc ttcccggcgg
     1441 ctcgtgggag gacctcgtcc gcgccgtgtt tcaggccgag caggagatgc tgccgcaccg
     1501 gcgctacccg ttcggcgcct tgcagcgcca gtgggggcag cgccccctct acgaggtcgc
     1561 gttcaattac atccacttcc atgtgatcga tgccctggcc gcctcgggga acctcgaggc
     1621 cctgggcttc aagcgggtgg agccgaccaa cctcgggctc caggtgcact tcggccgcag
     1681 ctccggctcg ggcctcatgg tgcagctcga ctacgacacc cagcgcttcg ccgcggccca
     1741 gatggccctg gtgggcggct actacgggcg gactttggct gccatggcgg cagggctgga
     1801 caccccgctc ctctcgccgg ccgaacgcca ggcgctgctc gccgagagca acgacacgcg
     1861 ggtggagcgc ccccggggcc tgctcctcca ccagctcgtg gcaccgggag cggtcgtctc
     1921 ccaggggtcc ctggtcctga gtcaggacga gctcgaggcg cgctccaacc ggctggcccg
     1981 ccatctcctg agcctgggcg tgagccccga gacgcgggtg gggatcagcc tggagcggac
     2041 gccggagctg gtggtggcgc tcctcgccgt gctcaaggcc ggcggcgcct acgtgccgct
     2101 cgacccgtcc cacccggcgg agcgcaccgc gctgatcctg gacgacgccg gggtcgccct
     2161 cctgctgacc ggcgagagcc tggagcgcga ccgggaggcc atcgcggcgc ggagcggggc
     2221 gccgctgccc ccgctggcga ccgaggacaa cctggcctac gtcatctaca cctccggctc
     2281 gaccggccgg cccaaggggg tgcagctctc ccaccgcgcg gtggtcaact tcctgctctc
     2341 gatggcggag cggccggggc tcacggcggc ggacgtggtg ctggccctga ccaccctcac
     2401 cttcgacatc gcggggctgg agatctacct gccgctgctg gtgggcggcc gggtggagat
     2461 ggtaacgcgc gaggaggcca ccgacggtgt ccgcctcgcc gcccggctgg cggcttgcgg
     2521 ggcgacggcc gtccaggcga cgccggccac ctggcggatg ctgctggagt cgggctggca
     2581 gggagacccc cgcttgaagg cgctgtgcgg cggcgaggcg ctgccgcgtg agctcgccga
     2641 ggcgctgcgg gcgcgggtgg gatcgctgtg gaacgtctac ggaccgaccg agaccgcggt
     2701 ctggtcggcg gccggcgagg tgacggaggg gaccggcccg gtgccggtcg gcccgccgat
     2761 cgccaacacc ggcctgtacg tcgtggctgc cgagaccctg gagctggtgc cggtgggcgt
     2821 ggccggggag ctgtggatcg gcggtgccgg cctcgcccgc ggctatctcg gacggccgga
     2881 cctgacggcg gagcggttcg tccccgatcc cttcgccgcc gcctcggggg aggcgggggc
     2941 gcgggcctac cgcaccggcg acctggtgcg ccgcctgccg gacccggccg gcgggctgga
     3001 gttcctgggg cgcgccgact tccaggtgaa ggtccggggc taccgcatcg agctggggga
     3061 gatcgaggcg gccctcgccc ggcaccccgc ggtggcgcag gcggtggcgg tggcccgggc
     3121 ggacggcggt gagaccctcc tcgcggccta tctggtggcg cgcgaggggg aggcggtccc
     3181 cggaccggcc gagctccgcg ccttcctgct gcgcggcctg ccggagtaca tggtccccgc
     3241 ggcgttcgtg gccctgccgg tcttcccgct gacccccagc ggcaagatcg accgccgcgc
     3301 actgtctggt cgggacgcag gtccggacgc ggtgcgccaa ggggagcgct acgtggcgcc
     3361 ggaggggccg gtcgaggagc tgctggcgca gatctgggcc gaggtgctgc gcgccgggcg
     3421 ggtcggcgcc gaggacggtt tcttcgagct cggcggccac tcgctgctgg cgacgcaggt
     3481 gatctcgcgc atccgtgcct ctctgggcgt ggagctgccg ctgcggcgcc tgttcgaggc
     3541 cggcaccgtg cgcggcctgg cgcgtgccgt cgaggcggcg cgccaggaga gcggcgaggc
     3601 gccgctgccg cccatcgccc gggtcccccg cgacggcgcc ctgccgctct cgttcgccca
     3661 g
//
LOCUS       DASPJI010000902         3991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_741254, whole genome shotgun sequence.
ACCESSION   DASPJI010000902 DASPJI010000000
VERSION     DASPJI010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741254"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VEL74_25550"
     CDS             <1..634
                     /locus_tag="VEL74_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HYG65965.1"
                     /translation="TARRLGEYAERARDSFNRRFWYEEGGHLYDVVDAEEGGDDPACR
                     PNQIFAVALDHPILERERWERVVQVVRERLLTPVGLRSLAPGHPDYKATYYGDLRSRD
                     AAYHQGTVWSWLIGPFVDAWLKVHPGDPAGARAFLEDFVPHLGEACLGSVSEVFDAEE
                     PYTPRGCVAQAWGVAELLRAWILTEPKRREDPRDAEPSLEDVAEQVVEIR"
     gene            1068..2876
                     /gene="dauA"
                     /locus_tag="VEL74_25555"
     CDS             1068..2876
                     /gene="dauA"
                     /locus_tag="VEL74_25555"
                     /inference="COORDINATES: protein motif:HMM:NF008660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HYG65966.1"
                     /translation="MPRRYQAPDGAPSFPLKLAAALRASLRHGYGRDDLRSDILAGLV
                     VGIVALPLSMALAVASGVAPQHGLYTAIVAGGLIALLGGSYVQVSGPTAAFVVILAPI
                     AARFGVGGLVLATLMAGLLLVAMGAARLGGLIQFVPYPVTTGFTAGIAVVIATLQLKD
                     FLGLTVERMPEHYLERVAALGRALPTARPADFAIGAFTLAVLLLWPRITRKVPAPLVA
                     LSLGGLLAALLTWWLPGLGIPFEVATIRSRFQYELGGVLHAGIPQLPPLPVLPWRLPG
                     PDGAPLGLSFGLVRDLMPSAFAIAMLGAIESLLSAVVADGMTGGSHDPDAELVAQGLG
                     NIAAPFFGGIAATGAIARTATNVRSGARSPLAAAFHALFVLVSVLALAPVLGYLPMAS
                     LAALLLLVAWNMSEAKHFAHALRVAPRSDVMVLVTCFGLTVVFDMVVSVTVGVILAAL
                     LFMRRMAEVSRVKLVDQHPLVSSGLPRDVLVYEVAGPLFFGAAAKAMSALQRVPSGVS
                     VVMLDISSVPVMDATGLVSLESAVDRLQSLGIYVVVAGVQEQPREVLARAGIERRGGG
                     IAVFGSVEEGVAAALAKSRLSRGGDPAVPAAGLAGS"
     gene            complement(2881..3117)
                     /locus_tag="VEL74_25560"
     CDS             complement(2881..3117)
                     /locus_tag="VEL74_25560"
                     /inference="COORDINATES: protein motif:HMM:NF038153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I lanthipeptide"
                     /protein_id="HYG65967.1"
                     /translation="MKRRTKKLSLNRETLRHLQADALREAAGRGPGCTMEDRTTCVCT
                     FDCDSGGAGTQTCGCPTGLNCPTDTLLDRTCCDC"
     gene            3290..3841
                     /locus_tag="VEL74_25565"
     CDS             3290..3841
                     /locus_tag="VEL74_25565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HYG65968.1"
                     /translation="MTTDSQEGRLLAALAAGDREAAERLVERTYRGVYTLLRRLCGDA
                     DLAADLTQETYRKAWDSLSGFNGRAQFSTWLCRIAYNTFLNHLRRPRRLVPLEDRHET
                     TVADPAPAADELVGRAVAGDRLRRAVLDLPEDLRFTVTALFWGDLPVREVARQEGITT
                     VGVRKRLKRALALLAKALEEDVR"
BASE COUNT          481 a         1437 c         1437 g          636 t
ORIGIN      
        1 gacggcccgg cgcctgggcg agtacgccga gcgcgcccgc gactccttca accgccgctt
       61 ctggtacgaa gaggggggcc acctctatga cgtcgtggac gcggaggagg ggggcgatga
      121 cccggcgtgc cggccgaacc agatcttcgc ggtcgcgctg gaccacccga tcctcgagcg
      181 ggagcgttgg gagcgggtgg tgcaggtcgt ccgcgagcgc ctcctgaccc ccgtcggcct
      241 gcgctcgctg gcgcccggcc atcccgacta caaggccacc tactatggcg acctccggtc
      301 ccgcgacgcc gcctaccacc aggggacggt ctggagctgg ctcatcggcc ccttcgtcga
      361 cgcctggctg aaggtccatc cgggggaccc ggccggcgcc cgcgccttcc tggaggactt
      421 cgtgccgcac ctcggcgagg cctgcctcgg ctcggtcagc gaggtcttcg acgccgagga
      481 gccctacacc ccccgcggct gcgtcgccca ggcctggggc gtcgccgagc tgctgcgcgc
      541 ctggatcctc accgagccga agcgccggga ggacccgcgg gacgcggaac cgagcctgga
      601 ggacgtcgcg gagcaggtgg tcgagatccg ctaggccttc accctctgcc ggcggcccgg
      661 gattcttccc gaaccctccg ggagccgtcc atctccaccg gacacgatcc ggagcgtccg
      721 gaaagcttcc cggctcatcc ggactccatc cttttgaatt ggacgccatc cggagcgtct
      781 ggaaatcatc cggttgaatg ggatgcgttc cgaagcgccc gggaagcttc ccgagccgtc
      841 cggaccgcag ccgagagact cggacgcgat ccggagcatc cgggaagctt cccggggctc
      901 gcggattcca tccgccgcgg cgggacggga tccgcggccc gccgcgcgga gccgaggcgt
      961 ccggatgcgc tccgcgccgg ccggccggag tcgaggggac ccgggagccg gggcaagggg
     1021 ccggcgcggt acaatcccga ggccacatct tcgcgagacg tatccccatg ccccgcagat
     1081 accaggctcc ggacggagcc ccttcgttcc ccctgaagct cgccgcggct ctccgggcga
     1141 gcctccgcca cggatatggc cgtgacgacc tgcgctcgga catcctggcg ggcctggtcg
     1201 tcggcatcgt ggcgcttccc ctgtccatgg ccctggccgt ggcctcgggg gtggcgccgc
     1261 agcacggcct ctacacggcc atcgtcgccg gcggtctgat cgcgctcctc ggcggctcct
     1321 atgtccaggt gtccggcccc accgcggcct tcgtggtgat cctcgccccg atcgccgccc
     1381 ggttcggggt cggcggcctg gtcctcgcca ctctcatggc cggcctcctc ctggtcgcca
     1441 tgggggcggc gcgcctgggc gggctcatcc agttcgtccc ctacccggtc accaccggct
     1501 tcacggcggg gatcgcggtg gtcatcgcca ccctccagct caaggacttc ctcggcctca
     1561 ccgtcgagag gatgcccgag cactacctgg agcgggtggc ggcgctcggc cgcgccctgc
     1621 ccacggcgcg gccggcggac ttcgccatcg gcgcgttcac cctcgcggtc ctgctcctct
     1681 ggccgcggat cacccgcaag gtgccggcgc cgctggtcgc cctgtcactg ggagggctcc
     1741 tcgccgcgct cctgacctgg tggcttcccg gcctggggat ccccttcgag gtggcgacga
     1801 tccgcagccg cttccagtac gagctcggcg gggtgctgca cgcgggcatc ccgcagctcc
     1861 cgccgctgcc ggtcctcccc tggcgcctgc cggggccgga cggggcgccc ctcggcctct
     1921 ccttcggcct ggtgcgggac ctgatgccgt cggccttcgc catcgccatg ctgggggcga
     1981 tcgagtcgct gctctcggcg gtggtggcgg acggcatgac cggcggctcc catgaccccg
     2041 acgccgagct ggtggcgcag gggctgggca acatcgccgc ccctttcttc ggcggcatcg
     2101 cggccacggg ggccatcgcc cgcacggcga ccaacgtccg ttccggcgcc cggtcgccgc
     2161 tggccgccgc cttccacgcg ctgttcgtcc tagtgtcggt gctggcgctg gcgccggtcc
     2221 tgggctacct gcccatggcg tcgctcgccg ccctgctcct cctcgtcgcc tggaacatga
     2281 gcgaggccaa gcacttcgcc catgccctgc gggtggcgcc gcgcagcgac gtcatggtgc
     2341 tcgtgacctg cttcggcctg acggtggtct tcgacatggt ggtgtcggtc accgtcgggg
     2401 tgatcctggc ggcgctcctc ttcatgcgcc gcatggccga ggtctcgcgg gtgaagctgg
     2461 tggaccagca cccgctggtc tcctcgggcc tgccacggga cgtgctggtc tacgaggtgg
     2521 cgggcccgct cttcttcggc gccgcggcca aggcgatgag cgctctgcag cgggtcccct
     2581 cgggggtgag cgtggtgatg ctcgacatct cctcggtgcc ggtcatggac gccaccggcc
     2641 tggtgagcct ggaatccgcg gtggaccgcc tccagtcgct ggggatctac gtggtggtgg
     2701 ccggggtcca ggagcagccc agggaggtgc tcgcccgcgc cgggatcgag cgccggggtg
     2761 gggggatcgc cgtcttcggt tcggtggagg agggggtggc ggcggcgctc gcgaagtcgc
     2821 ggctgagtag agggggcgac cccgcggtgc cggctgccgg cctcgcgggg tcttgagcgg
     2881 tcagcagtcg cagcaggtcc ggtcgagcag ggtgtcggtg gggcagttga gccccgtcgg
     2941 gcaaccgcag gtctgggtgc cggcgccgcc gctgtcgcag tcgaacgtgc agacgcaggt
     3001 cgtcctgtct tccatcgtgc agccggggcc gcggcccgcc gcctcgcgga gcgcgtccgc
     3061 ctgcagatga cgcagggtct cgcggttcag gctcagcttc ttggtccgtc gcttcatcaa
     3121 gacctcctct ggatagggtg ggtcatgcca gcctcttcta aatagattgg tgcatgggtc
     3181 ttctgatccg agacgatatt ccggtttccc ttctccttcc tggcgtgtcc aacgggatag
     3241 gatggatacg acacggacag cgactctgac gagctggtgg ggaggcgacg tgacgaccga
     3301 ctcccaggag gggcggctgc tcgccgccct cgcggccggc gaccgggagg cggccgaacg
     3361 cctggtcgag cggacctatc gcggcgtcta cacgctgctg aggcggctgt gtggcgacgc
     3421 ggacctggcg gcggacctca cccaggagac ctaccgcaag gcctgggact ccttgtccgg
     3481 gttcaacggc cgggcccagt tctccacctg gctctgccgc atcgcctaca acaccttcct
     3541 caaccacctg cgccggcccc gccggctcgt gcccctggag gaccggcacg agacgacggt
     3601 cgccgatccc gcgcccgccg ccgacgagct ggtggggcgg gcggtggcgg gggaccggct
     3661 ccggcgggcg gtcctcgacc tgccggaaga cctgcgcttc accgttaccg ccctcttctg
     3721 gggcgacctc ccggtgcggg aggtcgcgcg ccaggagggg atcacgacgg tgggggtccg
     3781 caagcgtctg aagcgtgccc tggcacttct cgccaaggcg ctcgaggagg atgtccgatg
     3841 agcaggatgg acgagcgcgc ggtcgcccgg aagctggccg agcgagacga tatcgagccc
     3901 ccggcggggc tcctcgagaa gatccggagc gagatcccgg ccgagatccg ggtctccccc
     3961 gagctggtga agacggtggg cggcgcggtg g
//
LOCUS       DASPJI010000903         3099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_514393, whole genome shotgun sequence.
ACCESSION   DASPJI010000903 DASPJI010000000
VERSION     DASPJI010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514393"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="VEL74_25570"
     CDS             <1..1457
                     /locus_tag="VEL74_25570"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HYG65969.1"
                     /translation="KDLSPSQVAALEKSRQKTRHVLGEDLKCTIRAANGVVGRVRAQI
                     KAQALLHSVLVSTPRDRGAVPFALDADGHLYVAREEDRARLRNLPLTGGQAGEQDNDL
                     AGEPWVVASKEEPETGLRFGIARPIGQALEEMRRTAARNFGFGLGLVGLCLAGVFPLS
                     GRISRNLESLTAGVEQIAHGDLTARVTVRSRDEFGRLAGAFNRMAFDLSRHQEQLLEQ
                     ERLRKEQELERRLLEAEHARKSRELEQARQFQLSLLPRELPSRPDLEVAVHTRTAAEV
                     GGDYYDFLLGEDGTLTVAIGDATGHGAAAGTMVTAAKGLFTGGAAGTGPARFLGHANS
                     VLKRMQLGRMAMAMSVLQLRGRRLTMSAAGMPPVLLHRAATGAVEEICLEATPLGALA
                     DCVYPERELELEPGDTLLLLTDGLPELLGEGGEPLGYPRLHELFAAAAGREPQEVIRH
                     LAAAADTWRGAEAPVDDMTFVVLQVRKERGVARV"
     gene            1518..1901
                     /locus_tag="VEL74_25575"
     CDS             1518..1901
                     /locus_tag="VEL74_25575"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HYG65970.1"
                     /translation="MRADPKLFNPRLVLLLTFVVLAPSLRLWSGHHLLPTHSQQPDPD
                     QTLTVWVLPDAIYIEETLVAEADLSRVLGEVRGSYPAAAVIVRGDSADRRLSYGRVRR
                     VLQLVNQAGFLRASLSPGTPPRTAG"
     gene            complement(1958..2818)
                     /locus_tag="VEL74_25580"
     CDS             complement(1958..2818)
                     /locus_tag="VEL74_25580"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HYG65971.1"
                     /translation="MKHETTPTRRRAATFLLSVLATVGLVAPLLAQPSDALLRNFMRS
                     GDYLLEVDGKAVPNAEIYQNNSIPAVLILTSSLSAPVLLNPRAGTVETVPIMKVLKKA
                     DGSADLSADAATQPQGEFQVVEGNAAFTLQGRKAVLKVRPSLLGLRRSADLKAYSPDY
                     QRVANTYTPNAQALATLKKETRPVTVRTYFGSWCPHCREHVPLLMKVEDQLKGSPSKI
                     KFEYMGLEKGLREPVVKQLGLKGVPTGIVLYNGKEIGRLNSDADWNAPEAALTRILAA
                     AGKSASKGKK"
     gene            complement(2859..>3099)
                     /locus_tag="VEL74_25585"
     CDS             complement(2859..>3099)
                     /locus_tag="VEL74_25585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65972.1"
                     /translation="NPQNPRLWVLSASEDTVHRAFFWQWGPSFEVVETPAELMRPLYR
                     RLPRSFMVKDGRVTQTFPGMPPLTTPANPQPSAGS"
BASE COUNT          435 a         1078 c         1123 g          463 t
ORIGIN      
        1 ggaaggatct gagcccgtcc caggtggcgg cgctggagaa gagccgccag aagacacgcc
       61 acgtcctggg cgaggacctc aagtgcacca tccgcgcggc gaatggggtg gtcgggcggg
      121 tccgggcgca gatcaaggcc caggccctcc tccacagcgt cctcgtctcg acccccaggg
      181 accgcggcgc cgtgcccttc gctctggacg ccgacggtca tctctacgtc gcccgcgagg
      241 aggaccgcgc gcggctccgg aacctgccgt tgaccggcgg gcaggccggt gagcaggaca
      301 acgacctggc cggcgaaccc tgggtcgtcg ccagcaagga ggagccggag accgggctgc
      361 gcttcggcat cgcgcgcccc atcggtcagg cgctggagga gatgcgccgg accgcggccc
      421 gaaacttcgg cttcggcctg gggctcgtcg gcctctgcct ggccggggtc ttcccgctgt
      481 ccggccgcat cagccgcaac ctcgagagcc tgaccgccgg cgtcgagcag atcgcccacg
      541 gcgacctgac ggcgcgggtg acggtccgct ccagggacga gttcggccgc ctggccgggg
      601 cgttcaaccg catggccttc gacctgtccc ggcaccagga gcagttgctg gagcaggagc
      661 gcctgcgcaa ggagcaggag ctggagcggc ggctgctcga ggccgagcac gcccgcaaga
      721 gccgcgagct ggagcaggcg cggcagttcc agctctccct gctgccccgg gagctgccgt
      781 cgcggcccga cctggaggtc gccgtccaca cccgcaccgc cgccgaggtg gggggcgact
      841 actacgactt cctgctgggg gaggacggga cgctcaccgt ggccatcggc gacgccaccg
      901 gccacggcgc cgcggcgggc accatggtga ccgcggccaa ggggctgttc accggcgggg
      961 cggccggcac cggcccggcc cgcttcctcg gccacgccaa cagcgtcctc aagcggatgc
     1021 agctcgggcg catggccatg gccatgtcgg tcctccagct ccgcggccgg cgcctcacca
     1081 tgtcggcggc cggcatgccg ccggtcctgc tccaccgggc ggccacgggc gcggtggagg
     1141 agatctgcct ggaggccacc cccctgggcg cgctggccga ctgcgtctat cccgagcggg
     1201 agctggagct ggagcccggc gacacgctcc tgctcctcac cgacggcctg cccgagctgc
     1261 tgggcgaggg gggagagccg ttgggatacc cgcgtctcca cgagctgttc gccgccgccg
     1321 ccggccggga gccgcaggag gtcatccgcc acctcgccgc cgccgccgac acctggcggg
     1381 gggccgaggc gccggtggac gacatgacgt tcgtggtgct ccaggtcagg aaggagagag
     1441 gcgtggcgcg cgtttagaga gacgtcgggg aaccggatgc ccggtcgacc cttctaatcc
     1501 tggtggagac gttcggattg cgggccgacc ccaagctgtt caacccccgg ctggtgctcc
     1561 tcctcacctt cgtggtgctg gccccctcgc tacgcctgtg gagcggccac catctgctgc
     1621 cgacccattc gcagcagccc gacccggatc agaccctgac cgtgtgggtc ctgccggacg
     1681 ccatctatat agaagagacg ctggtggcgg aggccgacct gtcgcgggtg ctcggtgagg
     1741 tccggggctc gtaccccgcc gccgccgtga tcgtccgcgg ggacagcgcc gaccgccggc
     1801 tgagctacgg gcgggtccgc cgggtcctcc agctcgtcaa tcaggccggc ttcctccgcg
     1861 cctcgctctc ccccggcacc cctcctcgca ccgccggctg aaaaagaaaa cggcggggcc
     1921 gcccccgccg tttcgagact ccggagctcc ggagagccta cttcttgccc ttggacgccg
     1981 acttgccggc cgcggcgagg atgcgggtga gagccgcctc gggcgcgttc cagtcggcat
     2041 cgctgttgag ccggccgatc tccttgccgt tgtagaggac gatgccggtg ggcactccct
     2101 tgaggccgag ctgcttgacg accggctcgc gcagcccctt ctccagaccc atgtactcga
     2161 acttgatctt ggagggggag cccttgagct ggtcctcgac cttcatcagc agcggcacgt
     2221 gctcgcggca gtgcgggcac cacgatccga agtaggtccg caccgtcacc ggccgggtct
     2281 ccttcttgag ggtggccaat gcctgggcgt tcggggtgta ggtgttggcg acccgctggt
     2341 agtccgggct gtaggccttg aggtcggccg acctgcggag gccgaggagc gacggccgga
     2401 ccttgaggac ggccttcctg ccctgaaggg tgaaggccgc gttgccctcg accacctgga
     2461 actcgccctg gggctgcgtc gcggcgtcgg cgctcaggtc ggccgagccg tccgccttct
     2521 tgagcacctt catgatcggg acggtctcga ccgtgccggc gcgagggttg agcagcaccg
     2581 gcgcggacag gctcgaggtg aggatcagca ccgccgggat gctgttgttc tggtagatct
     2641 cggcgttggg caccgccttg ccgtcgacct ccagaagata gtcgccgctg cgcatgaagt
     2701 tgcgcagcag ggcgtcggac ggctgcgcca ggaggggcgc gaccaagccg acggtcgcga
     2761 ggaccgacag gaggaacgtt gccgctctgc ggcgggtggg ggtcgtctcg tgcttcatat
     2821 ccatggcctc tcgatgggtc tcaaggtttt caggccgact aggagccggc ggaaggctgg
     2881 ggattggctg gtgtggtgag cggaggcatg cccgggaacg tctgggtcac tcgcccgtcc
     2941 ttgaccatga aggagcgggg caggcgccgg tagagggggc gcatcagctc cgccggcgtc
     3001 tccacgacct cgaacgacgg gccccactgc cagaagaacg cccgatgcac cgtgtcctcc
     3061 gacgcggaga gcacccagag cctcgggttc tgggggttg
//
LOCUS       DASPJI010000904        15445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_514756, whole genome shotgun sequence.
ACCESSION   DASPJI010000904 DASPJI010000000
VERSION     DASPJI010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 15445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15445)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15445
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514756"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1017)
                     /locus_tag="VEL74_25590"
     CDS             complement(91..1017)
                     /locus_tag="VEL74_25590"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65973.1"
                     /translation="MILVVGSASDTVASALVRQLRETGHPFQVLDEDHPERYSVQPDR
                     VAGRPGYRVLGGGCDGASPVGSIFLRHAVAGRLDPGRVASLGKLHEQLNRMLAVARCP
                     TVNLPANAGSNYSKPFQVGLMAEAGFDVPRSLVTNIPENARRFYDELGGRVIFKGISN
                     VATFAQVLTPDKLDRLDRLPDCPTLFQEYVAGVDYRVHVVGDEAFVTRLRSRGEDYRR
                     DARAGDPDLIIEPASMDERTVARCIEITRRLGLVVSGIDFKESPEGRLVALEVNPYPQ
                     FTFYEGRSGQPITRAVVSYLTRHPTDSSNVFA"
     gene            complement(1014..1706)
                     /locus_tag="VEL74_25595"
     CDS             complement(1014..1706)
                     /locus_tag="VEL74_25595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65974.1"
                     /translation="MFLILAESGDPLATQVENLFETQGRPARRLSEKELMEETPLAIE
                     RDGGRFGGLLRLPGGDLPLDQLSGVLVRMRRRWWPSSGFEPADQMFVYHETTAAVFCL
                     LAGLRCPVMNRFDLGWWLCDPAYPERLREELADRLGWSLIEADPECEPVRVYAVDGRL
                     IPASGQAVSFEDRAAALAEWQRRSGLRICRLDVSSGAEPALEAIDPCPSLVGESADLI
                     GRVAAAAVEGLA"
     gene            1805..4504
                     /locus_tag="VEL74_25600"
     CDS             1805..4504
                     /locus_tag="VEL74_25600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65975.1"
                     /translation="MRRWAKRLAFVSLLIAGLYAALYLLPANVFLGTSIGQRLLSRNP
                     QRLRVEWASARSLWPGEVEVRGLRIDGDAARIRWAVRVDRGQGRIDLPGLLSRRFQVS
                     GFEGTGVAARVDRHPPVPRTAKRRRSPWTLRMEGARVAGLRALEYGPLRLTGDGRAAG
                     SFSAVIGRQVRVDLERMEMTDGHLLARGRTIARGLSLRAGLRLGPYSPRRHPGAAGFD
                     FLSGALQASGAMEEVPVLRRIAGASTGPPRPGRLAVDVRVKRGAFVPGSRVELAPPVP
                     VGSPVAATAAVVRTPAGPRLRVDADLEGFTLHRRDGPPILTSESLRANALTTELRLSR
                     LIGQARDLRRGQTSAGWTLPAEVSVRGLRLTVPGRRASLEVTAGEARGRIDLPALLRR
                     EVLLDGVTGTEAHLRVAPEAPGPRPARTPAAGRSSWAVQSTATRFDGSCDAELGTLRL
                     QGGLRAAGDLSWKDGLLTLDLGTVTLKEGRLLRAAEPLAREVDLAGALRFGPYAPRRP
                     GFSWIGELSGAFEGRAKTEIPALQRLSGSPGPDARLTLDLRLDRGVLRSGTRMHLLQS
                     PMTVTASVPNGEKALHLQADLPNFTIRRRTGRPLLTAEALQATTEIPHLHLASLLGLG
                     QSLGGGGEGGRPPGAGDWERGREGWGKSAHEGAERGRPAPLAGWGLSPAEIHARALRL
                     TIPGERSSMELTAGAGHGKLDLPALFQREARFAGLSMTGSRLRLLPGTGEPPERPVAR
                     PGDPPLWKAELADSRLTGDCQVDLGGFGFTSSLQAAGDLTWSGEALAVQRAGVELAGG
                     LLSRDGETVAQDMSLMADAGSPPSIPPRWRGSARCGSSPGIGSSAAPSLPWASCAPTS
                     RRRAGCRWTAAAAWTRISTSTAAGCCRGAAFRCVRRSCVPSTC"
     gene            5016..5471
                     /locus_tag="VEL74_25605"
     CDS             5016..5471
                     /locus_tag="VEL74_25605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65976.1"
                     /translation="MREIGPRGRKARETAPGWWARLELSGTEMAWTRPMSLSGSVELA
                     MRDSDVLLTLFSRRKPYLRWFRRALHIEDLAARGDLRLEEGAVVLDRLVATGSGLELR
                     SRLRLSRDNPRGYLFLRRGLFKVGVELRDGRRHYRFRRPAQWFASRNAP"
     gene            5493..5975
                     /locus_tag="VEL74_25610"
     CDS             5493..5975
                     /locus_tag="VEL74_25610"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HYG65977.1"
                     /translation="MPDLDAQILRAVEEIPGISVLVLFGSRAKGTARPDSDLDVAVLP
                     DATVPLDDPTARRRLQTRIAVALADLAPDGRVDVVFLDEATDVLRQRVMEHGRVLICR
                     DREAWIELRVRTMREYGDREWVRDLDQREQRRRLEGGQPSGRSGKALESLRRIGRLSR
                     "
     gene            5956..6336
                     /locus_tag="VEL74_25615"
     CDS             5956..6336
                     /locus_tag="VEL74_25615"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HYG65978.1"
                     /translation="MEGYLAELRPFRAVSREEFVREPAMHHLAERLLHLACECVLDTA
                     QHVIAEGGYRQAATYKDLMQVLTEEGLIDAGLAERLKRWMGFRNALVHLYLELDHGRS
                     YDAIQEDLRDLEEFAAKIARLLPG"
     gene            6409..8127
                     /locus_tag="VEL74_25620"
     CDS             6409..8127
                     /locus_tag="VEL74_25620"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HYG65979.1"
                     /translation="MDYLDFVLRLDIGASGGLVVRLDSPAGQAEASFEPPPIREEVER
                     AAEEFERLLEEGQGQESFRDVSPRGVVAAIGRQKRIGDQLFKALFREPVRDRYQHSLG
                     SLEARPNLGLRIKVQMGLSDPSVAALQGLPWELLYRAEDGTFLGRMRRTSIVRYLDLP
                     LPGGQPPLPLPLRVLVVAARPRGVRPLPHVEREVLALRKLASQHSGLEVEKLMPPTLE
                     RLRQELLSGGFHVLHFLGHGAFREADGEGALYLEDDSGGPVAITGPMLADHLRDITSL
                     RLAVLNACQTARTSAPGPFGGVATALLRAGLPAVVAMQFPIGDRAAAAFSAGFYSRLV
                     AGDPLDAAVSEGRLKIHRDLPKAADWATPVLFLRSSDGRIFEPRQRSRKPWGAAAGLV
                     LLGALGLLVAQDRLMNVLVPESSRDSSSEARPTQAPERSASGQQEAGQSVSTPEPPLV
                     TRGVAFPSPSPSPSGGEPQSIKAPPSEPVARQQRRVPVTYTLSDRQPVYLPEIKAHLT
                     ASFNVYYGNLFVTLTLAPEKASGVKQIIAGTTTVEFQTEQGTCRIQVLALDEKARTVT
                     VLPSPS"
     gene            8193..9203
                     /locus_tag="VEL74_25625"
     CDS             8193..9203
                     /locus_tag="VEL74_25625"
                     /inference="COORDINATES: protein motif:HMM:NF036774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HYG65980.1"
                     /translation="MQARIRVLALVLLGFTLAFLSPDAARGQDFKSAVEQLARDLGNS
                     VPEGRTLTVAVSDFPDLQGGVTDLGRYVSERLVTRLSARPEKFRVIERRRLDQVLSEL
                     RFNLSDLVDPAKAQQLGKMLGVQAIVVGTLSDLGTNIDIDARIIDIGVNSTMPGVSVT
                     LSKDDTVRQLLERGRQMPGYGGGSSAGGSSSMPAPSSVLGFYESPEIRIEVDSLRVLK
                     NDEIIMTAYFSSSRSSGVTVALNDGGHGSWRYYPTYLVDELGQNFRLEKVSALRGNPL
                     FLPPGTRVLTTLTFDADYRGLGESVSLATDVDIFSADERGTAGKHLKTLNIWIRNMKP
                     SK"
     gene            complement(9213..9533)
                     /locus_tag="VEL74_25630"
     CDS             complement(9213..9533)
                     /locus_tag="VEL74_25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015184980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HYG65981.1"
                     /translation="MEERDLPAGLPLAQIEQFCRRWRITELSLFGSMLRGDARLDSDI
                     DLLVRFSPEADWSLFDHARMELELEKLLGRRVDLVSRSAIEKSPNWIRRQEILGSART
                     LYAG"
     gene            10416..10946
                     /locus_tag="VEL74_25635"
     CDS             10416..10946
                     /locus_tag="VEL74_25635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65982.1"
                     /translation="MRSFTDGSSPSAVPNAFTASFLDRLAERDEGPTAPEADVAGPWR
                     IEEVPCKGFGVFRVGESPARGFAPTVAFPSEWLALVAAGILPGTGREPMLILQKDRNP
                     DGTFSVSLENGEVVGRSHLFDEKMVDAMNVAIHLLRAPESLANVLDAAGLSPLERCGA
                     ILDERAGQRAARGEKE"
     gene            complement(11003..11413)
                     /locus_tag="VEL74_25640"
     CDS             complement(11003..11413)
                     /locus_tag="VEL74_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HYG65983.1"
                     /translation="MGPDSYVLDTSAIMALIEEEEGAARVQEVLEQESTILPWLVLME
                     VYYVTQQEKGSSEADRRYALLRQLPAEVPWNMDEATVLTAARFKAEFRLSLADALIAA
                     FARRREATLLHKDPEFEALAGQIELEALPYKSPH"
     gene            complement(11403..11633)
                     /locus_tag="VEL74_25645"
     CDS             complement(11403..11633)
                     /locus_tag="VEL74_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006853849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HYG65984.1"
                     /translation="METAVTKKGQTNIPAAIRKRHHIKEGDRLVWLDDGETIRVVPLP
                     SDPLAALRGSGRGEGLTAKLLRQRREDRERGS"
     gene            complement(11747..14848)
                     /locus_tag="VEL74_25650"
     CDS             complement(11747..14848)
                     /locus_tag="VEL74_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HYG65985.1"
                     /translation="MAAITRVSIRRPVATTMAFLILIVVGIVCLRSLPVDLLPQVEFT
                     QLTVRVSYPNVGPKEIEQIITDPIENAVSGLPNLERVTSESEEGSSRVRLEFSRGTNV
                     DEAANDLRAALDQLRNDLPIEATPPEILKLDLDRIEVVSLAATSTRDLQSLTRILEED
                     LARRFEQIPGVGAIEVRGGVYRQIRVELDRDRLKASGLTALGVQQALQAENVTLPGGN
                     VKSGFSDLYVRGLGEYQSVDQIARTVVATIAGKPVRLRDVAVVRDSYEDVRYLVEVNG
                     VPSVSLGIQKQSGANTVAVAEAIRAEIERVNAERDDLHLTVFADQSEFIRQSMDSVRQ
                     SALYGALLAIAVLYLFLRNRSSTVIIALSIPISVISTFGLLYFGGLTLNQMTFGGLAL
                     GVGMIVDNAIVVLESIVRKREEEGASMIEAARAGTADVAGAIVASTLTTCVVFLPVVF
                     TGTTSGALFQAMALVVVFSLMCSLLVALTLVPMLASRHLRLAETKTKEGRTDLMTRLE
                     GWYTRRLRHALQHRGRVFAVTGGLLLLALVLWPLIPVELAPPTEADEIDVDIEMAQGT
                     NIAVVRHYVGELERKVREVLPPGEVELVSTEVRGGDAEVELKLAPQAERSMSGEELAD
                     LLRDAVDGEIPGAEISVDAQPGLWILRRVFSSGGGEDDVEIELRGWDLERSDEIAADI
                     RRRIERLPGLTDVRVSRREGQREDNLVFDRARIAELGLTVRDVGRAVQANVGGVEAGR
                     FREEGLEYPIVVRLQPEDRLNSQDLRNVSLVTPDGAAVPLSSLVQWRRERAPVSIERV
                     DGQRVTYVTAGLEEEMTLGEAVESIRAALGDLRLPEGYSILYGGEFQEQQRAQRDFTV
                     AILMALVLVYMLMAGQFERYLDPLIVMLSVPMALIGVVPVLLLTGTTLNIQSIMGLVM
                     LIGIVVNNAIVLVDAINLQRREHQMGAADAVIEAGRLRLRPILMTTTTTVLGLLPLAL
                     GLGAGAEIQAPLARVVVGGLLASTLVTLLLIPVAYTSATGLVTRFKSRHWREEKEEER
                     ATA"
     gene            complement(14850..>15445)
                     /locus_tag="VEL74_25655"
     CDS             complement(14850..>15445)
                     /locus_tag="VEL74_25655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HYG65986.1"
                     /translation="EGSFSTIGEIDIADEQGRLSPGMFVTVDILHGQSERATLVPASA
                     LWEDPGSGVRGVYVVDGTGLREPAAAAAAAEAGTPAETGEVPEKPRGVQFRPVEVLAE
                     GRATAGITGLREGEWVVVVGQQLLARDGEAAARVRPTSWERVLQLQGLQREDLLQSFL
                     DKQQEWARTRGAEPPGNDEFIGRAGGAAPARPGAGGN"
BASE COUNT         2298 a         5285 c         5419 g         2443 t
ORIGIN      
        1 cgatctggcc gggacgatca cccttttcga tcatcggttc cttcttcttg tcagccatgc
       61 aatgcgctcc tttcgtgttg agtccgccgg tcaggcgaag acattgctcg aatcggtggg
      121 atggcgggtg aggtaagaga cgacggcgcg tgtgatcggt tggccgctgc gcccttcata
      181 aaaggtgaat tgcggataag ggttgacctc cagcgccacc agccggccct ccgggctctc
      241 cttgaagtcg atgccgctga ccaccaggcc gaggcgccgc gtgatctcga tgcaacgcgc
      301 gaccgtccgc tcgtccatgg aggccggctc gatgatcaga tcgggatcgc ccgcccgggc
      361 gtcgcggcga taatcctcgc cccgtgagcg caggcgggtg acgaaggcct cgtcgccgac
      421 cacgtggacc cggtagtcca cgcccgccac gtactcctgg aagagcgtcg ggcagtcggg
      481 aagccggtcg aggcggtcga gcttgtcggg ggtgagcacc tgggcgaagg tggcgacgtt
      541 gctgatcccc ttgaagatca cccggccgcc gagctcgtcg tagaagcgcc gcgcgttctc
      601 cgggatgttg gtcaccaggc tgcgcggcac gtcaaacccg gcttcggcca tcaatcccac
      661 ctggaagggt ttggagtagt tcgaaccggc gttggccggc aggttcacgg tcgggcagcg
      721 cgctaccgcg agcatccggt tgagctgctc gtgcagcttg cccagggagg cgacgcgccc
      781 gggatcgagt ctcccggcga cggcatggcg caggaagatc gagcccaccg gagaggcgcc
      841 gtcgcatccg ccgccgagga cgcggtagcc gggacgcccg gccacccggt ccggctggac
      901 cgaatagcgc tcggggtggt cctcgtcgag gacctggaac ggatgcccgg tctcgcggag
      961 ctggcggacg agcgccgacg cgacggtgtc gctggcggag ccgacgacga ggatcatgcg
     1021 agcccctcca cggccgccgc ggcgacccgg ccgatgaggt ccgcggactc gccgaccagc
     1081 gaagggcagg gatcgatcgc ctccagcgcg ggctcggcgc cgctcgatac gtcgagccgg
     1141 cagatgcgca gcccggatct ccgttgccat tcggcgagag cggcggcgcg gtcttcgaac
     1201 gagacggcct gtccggacgc ggggatcagc cgcccgtcca cggcatagac gcggaccggc
     1261 tcgcactccg ggtcggcctc tatcaatgac catccgagcc ggtccgccag ctcttcgcgc
     1321 agccgctcgg gataagcggg gtcgcacagc caccagccga gatcgaaacg attcatcacc
     1381 gggcagcgga ggccggccag cagacagaac acggccgccg tggtctcgtg gtagacgaac
     1441 atctgatcgg cgggctcgaa gccggaggac ggccaccagc ggcgccgcat ccgcaccagg
     1501 acgccggaca gttgatccag ggggaggtcg ccgccgggga ggcggaggag accgccgaac
     1561 cggccgccat cgcgctcgat ggccagcggg gtctcttcca tcagctcttt ctcggacagc
     1621 cggcgcgcgg ggcgtccctg ggtttcgaac aggttctcga cctgggtggc gaggggatcg
     1681 ccggactctg caaggatgag gaacatgaaa agacctcctc tgctcagagg ggcgatgcat
     1741 ttgctggaaa gttacagggg aggcacgcgt atgatctgag gagactcctt ggaccgaccg
     1801 acccatgcgc cgttgggcaa agcgactggc tttcgtttct ctcctgatcg cgggactgta
     1861 cgcggcgctc tacctgctgc cggccaacgt cttcctcggc acctccatcg gacagcggct
     1921 cctcagccgg aacccccagc ggctgcgcgt cgaatgggcc tcggcgcgga gcctctggcc
     1981 gggagaggtg gaggtccggg gcctgcgtat cgacggagac gccgcgcgga tccggtgggc
     2041 cgtgagggtc gaccgcggcc aggggcggat cgacctgccg ggcctgctga gccgccgatt
     2101 ccaggtgagc ggcttcgagg ggacgggcgt ggccgcgcgg gtggatcgcc atccgccggt
     2161 gccccggacc gcgaagcggc gccgctctcc ctggaccctg cgtatggagg gcgcccgggt
     2221 cgccggcctg cgcgcgctgg agtacggacc gctccggctg accggcgacg gccgcgccgc
     2281 gggctcgttc tcggccgtga tcgggcggca ggtccgggtg gatctcgaac gcatggagat
     2341 gacggacggc catctcctcg cccggggccg caccatcgcc cgcggcctct ctctccgcgc
     2401 cggcctgcgt ctcggcccct actctccacg ccggcatccg ggagccgccg gcttcgattt
     2461 cctgtccggc gccctccagg cgtcaggcgc catggaggag gtgccggtgc tccgccgaat
     2521 cgcgggtgcc tccacgggcc cgccacgtcc gggccggctc gccgtggacg tccgggtgaa
     2581 gcggggcgcg ttcgtgccgg ggagccgggt ggagctggcg ccgccggttc cggtgggatc
     2641 acccgtggcg gcgacggcgg cggtggtgcg gacaccggcc gggccgcggc tgcgggtgga
     2701 cgccgacctg gaaggcttca ccctccaccg ccgcgacggc ccgccgatcc tcaccagcga
     2761 gtccctgcgc gccaacgccc tcaccaccga gctgcgcctg agccggctga tcggccaggc
     2821 ccgtgacctg cggcgcggcc agacctccgc gggatggaca ctgccggcgg aggtgagtgt
     2881 ccggggattg cggctgaccg tccccggccg ccgcgcctcg ctggaggtca ccgccggcga
     2941 ggcccggggc cgcatcgatc tccccgcgct gctgcgccgc gaggtcctgc tggacggggt
     3001 gaccggcacc gaggcccacc tgcgggtcgc gccggaggca cctgggccgc gaccggcccg
     3061 gacaccggcg gccggccgct cctcctgggc cgtacagagc accgcgacgc gcttcgacgg
     3121 gagctgcgac gccgagctcg gcaccctccg gttgcagggt gggctacgcg ccgcgggcga
     3181 tctctcctgg aaggatgggc tccttaccct agacctcggc accgtcaccc tgaaggaggg
     3241 ccgcctgctc cgggcggcgg agccgctcgc ccgggaggtg gacctcgccg gagccctccg
     3301 cttcggtccc tacgcgccgc ggcgtccggg cttctcgtgg atcggagagc tgtccggcgc
     3361 cttcgagggc cgcgcgaaga ccgagatccc cgctctccag cggctgtccg gcagtcccgg
     3421 gccggacgcg cgcctcaccc tggatctgcg gctggaccgc ggcgtgctcc gctccggcac
     3481 ccggatgcac ctcctccagt cccccatgac ggtgaccgcc agcgtgccga acggcgagaa
     3541 ggctctccat ctccaggccg atctccccaa cttcaccatc cgccgccgca ccggacgtcc
     3601 cctcctgaca gccgaggcgc tccaggccac gaccgagatc ccccatctcc atctcgccag
     3661 cctgctcggt ctcggccagt ccctgggtgg gggaggcgag gggggccggc cgcccggcgc
     3721 aggcgactgg gagcgagggc gggaggggtg ggggaagagc gcacacgaag gagccgagcg
     3781 agggcggccg gctcccctcg ccgggtgggg gctctctccc gccgagatcc acgcccgcgc
     3841 cttacggctg acgatccctg gagagcgctc ctccatggag ctgaccgccg gcgccggcca
     3901 cggcaagctc gacctcccgg ccctcttcca gcgcgaggcc cgtttcgccg gcctctcgat
     3961 gaccgggagc cgtctgcgcc tcctgcccgg cacgggagag ccaccggaga ggcccgtcgc
     4021 tcgccccggt gacccaccgc tctggaaggc cgagctggcg gactcccggc tcaccggcga
     4081 ctgccaggtc gacctcggcg gcttcggctt cacctccagc ctgcaggccg ccggcgacct
     4141 gacctggagc ggcgaggccc tcgccgtcca gcgcgccggc gtggagctcg ccggcggcct
     4201 cctgagccgg gacggcgaga ccgtggccca ggacatgagc ctgatggcgg acgccggctc
     4261 gcccccttcg atccctccaa ggtggagggg ctcggcgcgt tgcggctcgt caccgggcat
     4321 tggcagctcg gcggcgccat cgctaccctg ggcttcctgc gcccctacct cgagaaggcg
     4381 agctggctgt cgctggacgg cagcggcagc ctggacgcgg atctccacgt cgaccgcggc
     4441 cggctgctgc cggggagccg cttttcggtg cgtccggcgc tcctgcgtgc cgagtacctg
     4501 ctgagccggg cgtccggcgt tgccgacctg accgccctgg tggagcaggg gccagaagga
     4561 gagaggctga gggtcggcgt ccatttcggc cgcttcgagg tcggagcccg ggacgagccg
     4621 gagggccggg cccacgtcac cgggcgcgat ctccgcctgg cgctcgtcac ccgcgacctg
     4681 gacctggccc gcgccgccaa agacctgcgg gtgcgtctcg acctgccgga cgccgacgtc
     4741 gccgacctga ccttctacaa cggctacctg ccggaaggat ccggagtgga gatcctctcc
     4801 ggcacgggcc gcctgagctc ctggctggag atggagacgg cgggccagac cgggcgaggc
     4861 gaggtggcgc tgcgctcgga cctcgtgcgg gtgcggctcc acgacatgga gctggcggga
     4921 gcgctctctc tctctcggcg ccgctcacca gcccggacct cagagggctg cgcttcgggc
     4981 tggacggcac gcgcctcgat ctccagcagg tggcggtgcg cgagatcggc ccccggggcc
     5041 ggaaggcgcg ggagaccgcg cccggctggt gggcgcgcct cgagctctcc ggcaccgaaa
     5101 tggcctggac ccgcccgatg tccctctccg gctcggtgga gctggcgatg cgggattcgg
     5161 acgtcctcct caccctcttc tcccggcgca agccctacct gcgctggttc cggcgggcgc
     5221 tccacatcga ggacctcgcc gcgcgggggg atctgcggct cgaagagggc gcggtggtgc
     5281 tcgaccgcct ggtcgccacc ggcagcggcc tcgagctgcg ctcgcgtctc cgcctgtccc
     5341 gagacaaccc gcgcggctac ctcttcctcc gccgcggcct cttcaaggta ggcgtcgagc
     5401 tgcgcgacgg ccgccgccac taccggttcc gccggcccgc ccagtggttc gccagccgga
     5461 acgccccgtg aaccggatgc tacgatgtcg ccatgccgga cctcgacgct cagatcctgc
     5521 gcgcggtgga ggagatcccg gggatctccg tcctggtgct cttcggctcc cgcgccaagg
     5581 ggacggcgcg cccggacagc gacctggacg tcgccgtcct gcccgacgcc accgttccgc
     5641 tggacgaccc caccgcccgg cggaggctcc agacgcggat cgccgtcgcc ctcgccgatc
     5701 tggcaccgga cggtcgggtc gacgtcgtct tcctggacga ggccacggat gtcctgcgcc
     5761 agcgcgtgat ggagcacggc cgggtcctga tctgccgcga ccgcgaggcc tggatcgagt
     5821 tgcgggtccg caccatgcgc gagtacggcg accgggagtg ggtgcgggac ctggaccagc
     5881 gggagcagcg ccggcgtctg gaaggaggtc agccgagtgg tcgatcgggc aaggctctcg
     5941 agtcgcttag acgcattgga aggctatctc gctgagctgc ggcccttccg ggccgtgagc
     6001 cgcgaggagt tcgtccgcga gcccgccatg catcacctgg cggagaggct cctccacctc
     6061 gcctgcgagt gcgtgctcga caccgcccag cacgtcatcg ccgagggcgg ataccggcag
     6121 gccgccacct acaaggacct catgcaggtc ctgaccgagg agggcctgat cgacgccggc
     6181 ctcgccgagc gcctcaagag gtggatgggc ttccgcaacg ccctcgtcca tctctacctg
     6241 gaactggacc acggcaggtc ctacgacgcc atccaagaag atctccggga cctcgaggag
     6301 ttcgccgcga agatagccag gctcctgcca ggctgagccg ggacccggag aaggactaac
     6361 cagcgccagc ctgggcggtg tctgacaagg caggacatcc gctcggcgat ggattacctc
     6421 gacttcgtgc tacgcctcga catcggcgct tcgggtggcc ttgtcgtccg cctcgactcg
     6481 ccagcggggc aggccgaggc ctctttcgag ccgccaccca tcagagagga ggtggagcgg
     6541 gccgcggagg agtttgagcg gctcctcgaa gaaggccagg ggcaggagtc ctttcgggat
     6601 gtgagcccgc ggggagttgt ggcggcgata ggccgccaga agcggatcgg cgatcaactc
     6661 ttcaaggccc tgtttcgtga gccggtgcgc gaccgctacc aacacagtct ggggtctctc
     6721 gaagcgcggc ccaatctggg cttgcggatc aaggtgcaga tggggctcag cgatccgtca
     6781 gtggcggctt tgcaggggct cccctgggag ctcttgtacc gagcggagga cgggacattc
     6841 ctgggaagga tgcgccggac ctctatcgtc cgttatttgg atcttcctct tcctggagga
     6901 cagcctccgc ttcctctgcc actacgcgtc ctcgtggtgg cggcgcgacc tcgcggagtc
     6961 aggcctttgc cgcacgtcga gcgggaagtg ctcgcgcttc ggaagctggc aagccagcac
     7021 agcggattgg aagtggagaa gctcatgccg ccaaccctgg agaggttgcg ccaagagctc
     7081 ctctcgggag ggttccatgt gcttcacttt ctgggacatg gggccttcag ggaagcggac
     7141 ggtgagggag ctctttatct ggaggacgac tcgggagggc cggtcgccat caccgggccg
     7201 atgctggccg atcacctccg agacatcaca tcgctgcggt tggcggtgct caacgcctgc
     7261 cagacggccc ggacctcggc gccgggccct ttcggagggg ttgccacggc cctgcttcgc
     7321 gctgggctcc cggccgtcgt ggcgatgcag tttcccattg gagaccgtgc cgcagccgcc
     7381 ttcagtgccg ggttctatag ccgcctcgtc gcgggcgatc cgctggatgc ggcggtgtcc
     7441 gaaggccgcc tcaagattca ccgtgacctt ccgaaggcgg ccgattgggc gactcccgtc
     7501 ctcttcctcc ggtcatcgga cggccggatc ttcgagcccc ggcagcggtc tcggaaacca
     7561 tggggcgccg cggcgggtct cgtacttctt ggtgcgcttg ggttgctcgt ggcgcaggac
     7621 cgcctcatga acgtgctcgt tccagaatca tctcgcgact cctcgtcgga ggcacgcccg
     7681 actcaggcac ccgaacggtc tgcgtcgggc caacaggaag ctggtcagag cgtttcgacc
     7741 ccagagccac ctctcgtcac gcgtggcgtt gcattcccga gcccctctcc ctcgccatcg
     7801 ggaggggagc cacaatccat caaggcgcca ccctcagaac cggtcgcacg acagcaacgc
     7861 cgggtccccg ttacgtatac cctcagcgac cgtcagccgg tctacttgcc ggagatcaag
     7921 gcgcacctga ctgcgagctt caacgtgtac tatgggaacc tcttcgtaac ccttactctg
     7981 gcccccgaaa aggcttcagg ggtgaagcag atcatcgcgg gcacgacgac ggtggagttc
     8041 cagacagagc agggaacctg ccgaattcag gtgctggcgc tggacgagaa agcgcgaacc
     8101 gttaccgtcc ttccaagtcc gtcctaacca cttccctcag tttcgggtct aactcttcgg
     8161 acctggctgt cgaatgaaga taagggggga gcatgcaagc gagaatccgt gtcttggcgt
     8221 tggtgcttct tggcttcact ttggcattcc tgtcccccga tgctgccagg ggccaggatt
     8281 tcaagagtgc agtcgagcaa ctcgcccgcg acctcgggaa tagtgtgcca gaaggacgga
     8341 ccctgactgt ggcggtctct gattttcccg atctccaggg tggtgtcacg gaccttggcc
     8401 gttatgtctc cgaacggctc gtgacccggc tcagcgcccg ccctgaaaag ttccgcgtca
     8461 tcgagaggcg gcgcctcgac caggttctgt ccgagcttcg cttcaacctg tcggatctcg
     8521 ttgatcctgc caaagcccag cagctcggga agatgctggg cgttcaggcg attgtggtcg
     8581 gcacgctgtc tgatcttggg accaacatcg atatcgacgc gaggatcatt gacatcgggg
     8641 tgaacagcac catgccgggg gtctcggtca cgctgagtaa agatgacacc gtgcggcaac
     8701 tgttggagcg cggccggcag atgcctggct acggaggagg ttcttctgcc ggtggttctt
     8761 cttcaatgcc ggctccgtct tcggttttgg gcttctatga gtctcccgag attcggatcg
     8821 aggtggactc acttcgggtc ctgaagaatg atgagatcat aatgacggcc tacttctcga
     8881 gctctcgatc cagcggtgtt actgtggctc tcaatgatgg ggggcacggc tcttggagat
     8941 attatccaac ctacctggtt gacgagttgg gacagaactt taggcttgag aaggtaagtg
     9001 cgctcagggg taaccctttg tttctgccgc ctggcaccag ggtgcttact acgctcacat
     9061 ttgatgctga ctataggggg cttggggagt cggtgtcact ggctacagac gttgacatct
     9121 tctccgccga tgagaggggg acggcgggca agcacctcaa gactctgaat atttggattc
     9181 ggaatatgaa gccgtccaag tgaggcggag agtcaaccgg catagagggt ccgggcactc
     9241 cccaggattt cctgccggcg aatccagttg gggctcttct cgatcgccga gcggctgacg
     9301 aggtccaccc ggcggccgag gagcttctcg agctccaact ccatccgggc gtgatcgaag
     9361 aggctccaat cagcctcagg ggagaagcgg acgaggaggt cgatgtcgct gtccagccgg
     9421 gcatcgcccc ggagcatgga gccgaagagg gacagctcgg tgatcctcca acgccggcaa
     9481 aactgctcga tctgagccag gggtaggccc gcgggcaggt ctctctcttc catctggata
     9541 gtatagaccc gtgcccgcag acgggctcgc atcccaccag gccccgtcgg tacgccgcgc
     9601 ctgccggcag acggagcgaa cctccgccgg ccggccagcc ggtgtcctcc tgtcggacgg
     9661 aggaaatcct cggggggcgc ggccttgggc acagcggcgc ccccgtcacg attcgattgt
     9721 caatgaacat cttgctgctc cactggttcg ttctgagtcc cggccggctt cgtgtactat
     9781 acgcaggctt cgggcggtcc gcaaggccaa atttgatact gaacggacta gagcgctctg
     9841 atagtccggt cgcgtggacg cgattcgacc cgcttgatgc gcagatcgcc ccttgggtcc
     9901 tcttcgtggc cccacggggc acctcagtga ccccatgggg ccagcctctc gccccacagg
     9961 gcgcggctgt cgccccttgg ggtgcgtgga ggccgaagcg gggcggggcg ggagggtgga
    10021 ggggcgaagc acagaccccg cggggcgaca gcaatccgag atggggcgaa gcgctcccgc
    10081 ggcggggcga gaggtcggcc cctcggggcg cggaggttct ttgacaatcg acgtacacgt
    10141 ccggagcgcg gcgccggtgg ccgggggtgc gccgcgcttg gatgtgcctt gggtcccgcg
    10201 aagggagacc gggagcaggt ggagacggca agaagatccc gcgaacaggg ttgacttctg
    10261 cccggtcttc gtactaggat tccgttatca ggttgtacgc agcacccgtg gcccggcgtc
    10321 gtgccggccc gcggcccaca gacaccgtgt ggcggcccgg gtgggagtta ggagctccct
    10381 cccgggcctc gccacacaaa agggaggaaa cccttatgcg tagcttcact gatggtagca
    10441 gtccgtccgc cgtacccaac gccttcaccg cctcgttcct tgaccggctg gccgagaggg
    10501 acgagggtcc caccgcgccg gaggccgacg ttgcgggtcc gtggcggatc gaggaggtcc
    10561 cctgcaaggg cttcggggtg ttccgggtgg gggagagccc ggcccggggc ttcgcgccca
    10621 cggtggcgtt cccctccgag tggctcgccc tggtcgccgc cggcatcctt cccggcaccg
    10681 gccgggagcc gatgctcatc cttcagaagg accgcaaccc ggacggcacc ttctccgtct
    10741 ctctggagaa cggtgaggtg gtggggcggt cccacctctt cgacgagaag atggtggacg
    10801 ccatgaacgt cgccatccac ctgctgcggg cgccggaatc gctggccaac gtcctggacg
    10861 ccgccggcct ctcgccgttg gagcgctgcg gcgccatcct cgacgagcgg gccggccagc
    10921 gggcggcgag aggtgagaag gagtaggggc ttcctgatgt agatcggcag cgccaccggc
    10981 ggggcgaccc gccggtggct gctcaatgag gggacttgta ggggagcgct tccaactcga
    11041 tctggccggc gagggcttcg aactcagggt ccttgtggag aagggtcgcc tcacggcgcc
    11101 gcgcaaacgc cgcaatcagc gcatcggcca gggaaagacg gaactccgcc ttgaagcgag
    11161 cggccgtgag caccgtcgcc tcgtccatgt tccaggggac ctccgcgggt agttggcgca
    11221 acaaggcata gcgacgatcc gcctcagagc tgcccttctc ctgctgggtt acgtagtaga
    11281 cctccatgag caccagccag gggaggatcg tgctctcctg ctccagcacc tcctgaactc
    11341 gcgcggcgcc ctcctcctcc tcgatcaagg ccatgatcgc cgaggtgtcg aggacgtacg
    11401 aatcaggacc cacgctcgcg gtcctctcgc cgctgtcgaa ggagcttggc ggtcagcccc
    11461 tctccccggc ctgaccctcg gagcgcggcc aggggatcgg aaggcagcgg caccacccgg
    11521 atcgtctccc catcatcgag ccacacgaga cggtcgccct ctttgatgtg atgacgcttg
    11581 cggatcgctg ccggaatgtt ggtctggccc ttcttggtga cagcggtctc cataacctct
    11641 ccttttcccg tcagtctagg ggagaagcgg cagagcggca aggcagacag atgaaactgc
    11701 aaggcactcg cttcggtggc cttgccgcgt ggccggtcca cgccccctac gccgtcgccc
    11761 tttcttcctc cttctcctcc cgccaatgcc gggacttgaa gcgggtcacc aggccggtgg
    11821 cggaggtgta ggcgacgggg atcaggagca gggtcaccag ggtcgaggcg agcagccctc
    11881 ccaccacgac gcgggccagc ggcgcctgga tctcggcgcc ggcgccgagg cccagcgcca
    11941 gcggcaggag gcccagcacc gtcgtcgtcg tggtcatcag gatcggccgc aggcgcagac
    12001 ggccggcttc gatcaccgcg tccgccgcac ccatctgatg ctcccggcgc tgcaggttga
    12061 tggcgtccac cagcacgatg gcgttgttga ccacgatgcc gatgagcatc accaggccca
    12121 tgatgctctg gatgttgagc gtcgtgccgg tcagcagcag caccggcacc acgccgatca
    12181 gcgccatcgg caccgagagc atcacgatca gcggatcgag gtagcgctcg aactgcccgg
    12241 ccatcagcat gtagaccagc accagggcca tgaggatggc gacggtgaag tcgcgctgcg
    12301 cccgctgctg ctcctggaac tcgccgccgt agaggatcga gtagccctcg ggcaggcgca
    12361 ggtcgcccag cgcggcgcgg atcgactcca ccgcctcgcc gagcgtcatc tcctcctcga
    12421 ggccggcggt gacgtaggtg acccgctggc cgtccacgcg ctcgatggag accggcgccc
    12481 gctcacggcg ccactggacg agcgacgaga gcggcaccgc ggccccgtcc ggcgtcacca
    12541 gggagacgtt gcggaggtcc tggctgttca ggcggtcctc gggctggagg cggacgacga
    12601 tggggtattc gagcccctcc tcgcggaagc ggccggcctc gacgccgccg acgttggcct
    12661 ggacggcgcg gcccacgtcg cgcacggtga gcccgagctc ggcgatgcgc gcgcggtcga
    12721 agaccaggtt gtcctcgcgc tgcccctcgc gccggctgac gcggacgtcg gtgaggcccg
    12781 gcaggcgctc gatgcgccgc cggatgtcgg cagcgatctc gtcggagcgc tccaggtccc
    12841 agccgcgcag ctcgatctcc acgtcgtcct cgccgccgcc ggagctgaag acgcggcgca
    12901 ggatccacag gccgggctgg gcgtcgacgc tgatctcggc gccggggatc tcgccgtcca
    12961 cggcgtcgcg caggaggtcg gccagctcct cgccggacat ggagcgctcc gcctgcggcg
    13021 ccagcttgag ctcgacctcg gcgtcgccgc cgcgcacctc ggtcgagacc agctccacct
    13081 cgcccggcgg caggacctcc cggaccttcc tctcgagctc gccgacgtag tggcggacga
    13141 cggcgatgtt ggtcccctgc gccatctcga tgtccacgtc gatctcgtcc gcctcggtgg
    13201 gcggcgcgag ctccaccggg atcagcggcc agagcaccag cgccaggagg aggagcccgc
    13261 cggtgacggc gaagacccgc ccccggtgct ggagggcgtg gcggagccgg cgcgtgtacc
    13321 agccttcgag gcgggtcatc aggtcggtcc tgccctcctt cgtcttcgtc tccgccaggc
    13381 ggaggtggcg cgaggccagc atcggcacca gggtgagcgc caccagcagg gagcacatca
    13441 gcgagaagac caccaccagg gccatcgcct ggaacagcgc gccgctcgtg gtgccggtga
    13501 agacgacggg gaggaagacg acgcaggtgg tgagggtcga ggcgacgatc gcccccgcca
    13561 cgtcggcggt gccggcgcgc gccgcctcga tcatcgaggc cccctcctcc tcgcgcttgc
    13621 ggacgatgct ctccagcacc acgatggcgt tgtcgacgat catccccacc cccagggcga
    13681 ggccgccgaa ggtcatctgg ttgagcgtca ggccgccgaa gtagaggagg ccgaaggtgg
    13741 agatcaccga gatcgggatc gagagcgcga tgatcaccgt gctcgaccgg ttgcgcagga
    13801 acaggtagag cacggcgatc gcgagcagcg ccccatagag cgccgactgg cggacgctgt
    13861 ccatggattg gcggatgaac tcgctctggt cggcgaagac ggtgaggtgg aggtcgtcgc
    13921 gctcggcgtt gacgcgctcg atctcggcgc ggatcgcctc ggccacggcc acggtgttgg
    13981 cgccgctctg cttctggatg cccaggctca ccgacgggac gccgttgacc tccaccaggt
    14041 agcgcacgtc ctcgtagctg tcgcgcacca cggccacgtc gcgcaaccgg accggcttgc
    14101 cggcgatggt ggcgacgacg gtgcgggcga tctggtcgac cgactggtac tcgccgaggc
    14161 cgcgcacgta gaggtcgctg aagccgctct tgacgttgcc gccgggcagg gtgacgttct
    14221 cggcctggag cgcctgctgg acgccaagcg ccgtgaggcc cgaggccttc agccggtcgc
    14281 ggtcgagctc gacgcggatc tgccggtaga cgccgccgcg cacctcgatg gcgccgacgc
    14341 cggggatctg ctcgaagcgg cgggccaggt cctcctccag gatgcgggtg agggattgca
    14401 ggtcgcgcgt cgaggtggcg gcgaggctca ccacctcgat gcggtcgagg tcgagcttca
    14461 ggatctcggg aggcgtcgcc tcgatgggca ggtcgttacg gagctggtcg agcgccgccc
    14521 gcaggtcgtt ggccgcctcg tcgacgttgg tgccgcggga gaactccagc cgcacccggc
    14581 tcgatccctc ctcggactcc gaggtgacgc gctccaggtt cggcaggccg gagaccgcgt
    14641 tctcgatggg gtcggtgatg atctgctcga tctccttcgg ccccacgttg ggatagctga
    14701 cgcggacggt gagctgggtg aactcgacct gggggagcag gtccaccggc aggctgcgca
    14761 ggcagacgat gcccaccacg atgaggatga ggaaggccat ggtggtggcc acgggccggc
    14821 ggatcgagac gcgggtgatg gcggccacgt cagttccctc ccgctccggg cctggcaggg
    14881 gcggcgccgc cggcccggcc gatgaattcg tcgttgccgg ggggctcggc gccgcgggtg
    14941 cgcgcccact cctgctgctt gtcgaggaag ctctggagga ggtcctcgcg ctgcaggccc
    15001 tggagctgca gcacgcgctc ccaggaggtg gggcggacgc gggcggcggc ctcgccgtcc
    15061 cgggccagga gctgctgccc gaccaccacc acccactccc cctcgcggag gccggtgatt
    15121 cccgccgtgg ccctcccctc ggcgagcacc tcgacgggcc ggaattggac gccgcgcggc
    15181 ttctcgggga cctcgccggt ttcggcgggg gtgccggcct cggcggcggc ggccgccgcc
    15241 gccggctcgc gcaggccggt gccgtccacc acgtagacgc cgcggacgcc gctgcccggg
    15301 tcctcccaca gggcgctcgc cggcaccagg gtggcccgtt cgctctgccc atggaggatg
    15361 tcgacggtga cgaacatgcc ggggctgagc cgtccctgct cgtccgcgat gtcgatctcg
    15421 ccgatggtgc tgaagctgcc ctctt
//
LOCUS       DASPJI010000905         2578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_742245, whole genome shotgun sequence.
ACCESSION   DASPJI010000905 DASPJI010000000
VERSION     DASPJI010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742245"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1641)
                     /locus_tag="VEL74_25660"
     CDS             complement(<1..1641)
                     /locus_tag="VEL74_25660"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HYG65987.1"
                     /translation="MPNRLRLCFAFVFLFLLIASARLPAQNVWFPTGPDGGKVTALAV
                     HPNTQRIVYAGTDGGGVFKSADGGRTWFPVNNGLFNLFIRDLVIDPQAPDTLYLAAGY
                     LPTVAGVYKTTDGGGIWRNVSGGLPTPPPFCGCGGLMPVTDLAIDPGTPRILYAVAGG
                     RVYKTERGGGWLSASSGLNDGGVLTVEVAPSSPNVLYAGTVTGVYTSANRGSTWSAAT
                     TGVGAQPVRALAVDPRNARTVYAGTDANGVFKSTNGGVSWSPASQGLLDRQVFALAID
                     PSNPANFWAGTGQTLYKSTDAAGSWRQVPTGVPPSPVGVLAVAPSAPNVIFAGPGEVP
                     WVKTGAGVFKSTNSGETWAAFQQGLKASIVNSLASAGAPGALFAGTSGGGVFRTDNGG
                     LDWQPRNAGLPQTRDVLSLATDPTDPEVVYAGIGGGGGVYKSADGGASWTFASTGLII
                     PATSNALVSPEDLDIDPENPSIVFAAGSGGFYRSTDGAATWERVAGMLRDSLSLAIAP
                     SDPSTIYAGGGPAFLPSTTPYPTLLRSRDGGTAWEALDS"
     gene            complement(1810..2412)
                     /locus_tag="VEL74_25665"
     CDS             complement(1810..2412)
                     /locus_tag="VEL74_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HYG65988.1"
                     /translation="MIRVLVAEDQGMVLGALAALLDLEPDIEVVARALDGREALRLAG
                     ETAPDVVLSDIEMPHVTGLELAAELRRQGSPARVVILTTFARSGYLRRALEAGAVGYL
                     LKDSPSERLAEAVRRAHAGLRTVDPDLAAAAWDDPDPLTDRERQVLRLAGDGLTTVAI
                     AAEVHLSEGTVRNYLSEAISKLGAANRVEAARVARQKGWL"
BASE COUNT          369 a          892 c          955 g          362 t
ORIGIN      
        1 cgagtcgagg gcctcccagg ccgtgccgcc gtcccggctc cgcaacagcg tcgggtaggg
       61 ggtcgtggac ggcaggaacg ccgggccgcc tcccgcgtag atcgtcgagg ggtcggaggg
      121 cgcgatggca agggagaggg aatcccgcaa catcccggcg acccgctccc aggtcgccgc
      181 gccgtcggtg ctccggtaga agccgccgct gccggcggca aagacgatgg aggggttctc
      241 cgggtcgatg tccagatcct cgggcgagac cagggcgttg gaggtcgcgg ggatgatcag
      301 cccggtggag gcgaaggtcc acgacgcgcc gccgtcggcg ctcttgtaga cgcccccgcc
      361 gccgccgatt ccggcgtaga ccacctcggg atcggtggga tcggtggcga gcgagagcac
      421 gtcccgcgtc tgggggaggc ccgcgttgcg cggctgccag tcgaggccgc cgttgtccgt
      481 acggaagacg ccgccgcccg aggtgccggc gaacagggcg cccggcgccc cggccgaggc
      541 caggctgttc acgatcgagg ccttcaggcc ctgctgaaag gccgcccagg tctccccgct
      601 attagtgctc ttgaagaccc ccgcccccgt cttcacccag gggacctcgc cggggccggc
      661 gaagatcacg ttgggtgcgg agggggcgac ggcgaggacc ccgaccgggc tcggcggcac
      721 acccgtgggt acctggcgcc agctcccggc cgcgtcggtg ctcttgtaga gagtctggcc
      781 ggtgcccgcc cagaagttgg ccggattgga gggatcgatc gccagcgcga acacctgccg
      841 gtcgagcagc ccctggctcg ccggggacca gctcacgccg ccgttggtgc tcttgaagac
      901 gccgttggcg tcggtgcccg cgtagaccgt ccgcgcgttg cggggatcga cggccagcgc
      961 cctcaccggc tgcgccccca cgcccgtggt cgcggcgctc caagtgctcc cgcggttggc
     1021 gctcgtgtag accccggtga ccgtgcccgc gtagaggacg ttaggggacg acggggccac
     1081 ctccaccgtc agcacgcctc cgtcattcag cccggagctc gcgctgagcc acccgccgcc
     1141 gcgctcggtc ttgtagaccc ggcctccggc cacggcatag aggatgcgcg gggtccccgg
     1201 atcgatcgcc aggtcggtga cgggcatcag gccgccgcag ccgcagaagg ggggaggcgt
     1261 gggcaacccg ccgctgacgt tgcgccagat gccgccgccg tcggtcgtct tatagacgcc
     1321 ggccacggtg ggcagatacc ccgccgcgag gtagagcgta tccggcgcct ggggatcgat
     1381 caccaggtcg cggatgaaga ggttgaacag gccgttgttg actgggaacc aggtgcgccc
     1441 gccgtcggcg ctcttgaaca cgccgccgcc gtcggtgccg gcgtagacga tgcgctgtgt
     1501 gttgggatgg accgccagcg cggtcacctt gccgccgtcc ggtccggtcg ggaaccagac
     1561 gttctgtgcc gggagacggg ccgaggcgat gagcaggaac agaaaaacaa aagcaaagca
     1621 gagtctgagc cgattcggca tggtgaaccc tccggagtct ctcctcgaga aagcggatcc
     1681 ccttctggag atcccttgat ccgtgcttct actctgtata cgaatgagac aggtggagcg
     1741 ttgagccgat gatcagaagt ggccgttgag gcggacctcg aggctcccgg ccggcagcag
     1801 cgggatggtc tacaaccacc ccttctggcg ggcgacgcgg gcggcttcga cgcggttggc
     1861 ggcgccgagc ttgctgatgg cttcggaaag atagttgcgg acggtccctt cggagaggtg
     1921 gacctcggcg gcgatggcga cggtggtgag cccgtcgccc gccaggcgca gcacctggcg
     1981 ctcgcggtcg gtcagcgggt cgggatcgtc ccaggcggcg gcggcgaggt ccggatcgac
     2041 ggtgcgcagg ccggcgtggg cgcggcgcac ggcctcggcc agccgctccg aaggcgagtc
     2101 cttcaggagg tagcccaccg cgcccgcctc cagcgcccgc cgcaggtagc cgctgcgggc
     2161 gaaggtggtg aggatgacca cccgcgccgg gctcccctgg cgcctgagct cggccgccag
     2221 ctccagtccg gtcacatggg gcatctcgat gtccgagagg acgacgtccg gcgcggtctc
     2281 ccccgccagc cgtagcgcct cgcggccgtc cagcgcccgc gccaccacct cgatgtccgg
     2341 ctcgaggtcg aggagggcgg ccagggcgcc cagcaccatc ccctggtctt cggcgacgag
     2401 gacgcggatc acgcgctctc caccgggact cctcctctcc cggggagggg gccggggagg
     2461 cgggagaggg gaccgggggg tgagggctcc agtttgccgg gcagggtgat caccaccgcc
     2521 gtcccctcct cgccccggcg ctcgagctgt ccgcccagcc cctcgacgcg ctcgcgca
//
LOCUS       DASPJI010000906         5866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermoanaerobaculia bacterium isolate SMAG_U12005
            k141_743476, whole genome shotgun sequence.
ACCESSION   DASPJI010000906 DASPJI010000000
VERSION     DASPJI010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37944929
            Sequence Read Archive: SRR7003764
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermoanaerobaculia bacterium (soil metagenome)
  ORGANISM  Thermoanaerobaculia bacterium
            Bacteria; Acidobacteriota; Thermoanaerobaculia.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 05:01:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,140
            CDSs (total)                      :: 5,096
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 44
            rRNAs                             :: 1 (5S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 2 of 34
            Pseudo Genes (incomplete)         :: 28 of 34
            Pseudo Genes (internal stop)      :: 5 of 34
            Pseudo Genes (multiple problems)  :: 1 of 34
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Thermoanaerobaculia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743476"
                     /isolate="SMAG_U12005"
                     /isolation_source="soil"
                     /db_xref="taxon:2651171"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="33.3 N 116.25 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VEL74_25670"
     CDS             <1..327
                     /locus_tag="VEL74_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYG65989.1"
                     /translation="DGIQRLNQEAAKAMAAGNRMGMSLRPEDAVRWITINPARSLGIG
                     DRTGSLEEGKMADVVIWSGNPFSVYSRAEKVYVDGALVYDRNDPARQPRTDFELGLMG
                     SPEVAQ"
     gene            399..1610
                     /locus_tag="VEL74_25675"
     CDS             399..1610
                     /locus_tag="VEL74_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017505003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HYG65990.1"
                     /translation="MAITGATVHTLGAAGKLEGATVLIEDGRIRAVGRDVAVPSGARR
                     IDAAGKVVTPGLFDSLSRLGLVEVGAVEGSVDTTVEDYDPVTAAFQVADAINPRSSLI
                     AVNRIEGLTRAVVAPEAGDSLIAGQAAIIHLGGPGDFLLRSPAAMFAVLGEAGAERAG
                     GARGAALLRLREALQDAEDYAENRAAFERGDRREYSVSRLDLEALVPVVRGELPLVVT
                     VNRASDIQAALRLAKELELKLILAGANEGWLVAREIAQAKVPVLVTPMENRPERFEIL
                     AATLENTARLHAAGVTVALMSGDAHNARNIRQAAGNAVAYGMPWDAALAAMTVVPARL
                     WGLADRYGTLEPGKDADVVIWDGDPLEITTFADRVFIRGVEIPMRSRQTELRDRYKDL
                     SGDLPPAYRNP"
     gene            complement(1663..2868)
                     /gene="lhgO"
                     /locus_tag="VEL74_25680"
     CDS             complement(1663..2868)
                     /gene="lhgO"
                     /locus_tag="VEL74_25680"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HYG65991.1"
                     /translation="MNARTADVAIVGGGIVGLATAYRFAERYPGRRVVVLEKEPEVAL
                     HQTGRNSGVLHSGIYYKPGSLKAVNCREGKRAMEEFCAREGIAYELCGKVIVALGEPE
                     LPALARIHERGQENGVRCEVIGPERLRELEPHAAGIRAIHVPETGIVDYPEVCRRLVQ
                     RIRESGGAVVTGARVTGIRRIDPTVTVETEAGDYEAGYVVNCAGLYCDRVAKLSGQDP
                     GAKIVPFRGEYFELERDAWPLCRNLIYPVPDPSFPFLGVHFTRMIHGGVECGPNAVLA
                     FAREGYTKRDIHPADLLESLTYAGFLRLASRHWRTGMGEMWRSFSKKAFVRALQRLVP
                     GIREEHLHPAPAGVRAQAVTPDGLPVDDFLIAESERVVNVLNAPSPGATASLNIGRLI
                     VDKLAARMG"
     gene            complement(2903..3853)
                     /locus_tag="VEL74_25685"
     CDS             complement(2903..3853)
                     /locus_tag="VEL74_25685"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HYG65992.1"
                     /translation="MKDALTVEGDRAGLTLAALVREIAGEGTSWSKARDLCRAGRVLV
                     DGAVETDPARRAIAGERVELRLAGPAVLRGQRGPLPPDAVVHLDADVVVVNKPAGLLT
                     VPFERDDRDTLLAFTRVAVRRLGDPKPTLRAVQRLDKETSGLVVFARNVRAQRHLQEQ
                     LGRHDVTRRYLAIAHGRVESATFDTLLLADRGDGLRGSWNPRRHGRGEPPPDAKPAVT
                     RVEALERLAEATLVACELETGRQHQIRIHLSEAGHPLVGEAVYIRDWRGPRLPAPRPM
                     LHATVLGFLHPRSGRAVRFEAPPPADFNAVLGRLRQPAVR"
     gene            3915..5264
                     /locus_tag="VEL74_25690"
     CDS             3915..5264
                     /locus_tag="VEL74_25690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HYG65993.1"
                     /translation="MIRSEPVRQELRATLRLAAPVVLVQLGSMLMGMVDTLMVGHLSA
                     EALAAAALAHIFVITVLMLGAGILQALDPLITQAYGAGDVHGVGDHLLRGIVMAAALS
                     LPISLLMLDTREALALLGQQPEVIDGAAGYIRAVIWGNLPFFLFTVVRLTLQTQSIVR
                     PAVMAMVIGNVVNVIVNYSLIFGKFGMPALGVVGSAYSTALSRWCMCLYLVIASRRAL
                     APYWRGFTGAAASLRHYTRLLRLGVPIGLHNSLELCLFLTVALLIGQMGVAQLAGHQI
                     ALNLSAISFMVPLGIGGAAATRVGNAIGRGDMPGARRAAAVSLGLGAGAMALFGLLFA
                     SAPRPLASLYTDDAAVLAMAVALLPIAAAFQIFDGVQVVSAGILRGSADTTYPAALAL
                     FGFWALGLPGGWYMAVPLGLGPRGLWWGLTLGLGAVSALFVLRIVTRFRSHISREMG"
     gene            5274..5675
                     /locus_tag="VEL74_25695"
     CDS             5274..5675
                     /locus_tag="VEL74_25695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HYG65994.1"
                     /translation="MTPLRLQVLAERFAVCRLPPDSPIPPRPQACGLWSATLTGNELS
                     LVLPESAAPPGVPTERGWRALEVAGPLDFALTGVMASLSSPLAAAGISLFVVSTYDTD
                     YVLVRDHDLGRAKEALEAAGHQVAEGPGGGS"
     gene            5672..>5866
                     /locus_tag="VEL74_25700"
     CDS             5672..>5866
                     /locus_tag="VEL74_25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004506541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HYG65995.1"
                     /translation="MTFKDHFSGHAADYASFRPGYPAVLFERVAALPRHRRLAWDCGT
                     GSGQAAVGLAEHFERVIATDP"
BASE COUNT          740 a         2199 c         2082 g          845 t
ORIGIN      
        1 gacggcatcc agcgcctcaa ccaggaggcg gccaaggcca tggcggccgg caaccgcatg
       61 gggatgagcc tgcgccccga ggacgccgtc cgctggatca ccatcaaccc ggcgcgctcg
      121 ctgggcatcg gcgaccgcac cggctcgctc gaggagggga agatggccga cgtggtgatc
      181 tggagcggca acccgttcag cgtctacagc cgggccgaga aggtctacgt cgacggcgcc
      241 ctggtctacg accgcaacga cccggcgcgc cagccgcgca ccgacttcga gctcggcctg
      301 atgggatctc cggaggtggc ccagtgaccg gcgcgcgcag ggcgctcgcc gccctctcac
      361 tgacgcttgt agcggcctcc cccgtccggg cggagacagt ggccatcacc ggcgcgaccg
      421 tccacaccct gggcgccgcc ggcaagctcg aaggggcgac ggtgctcatc gaggacggcc
      481 gcatccgcgc cgtgggccgg gacgtggcgg tgccatcggg tgcccggcgg atcgacgcgg
      541 ccggcaaggt ggtgacgccc gggctcttcg actccctctc ccgcctcggc ctcgtcgagg
      601 tcggcgccgt cgaggggtcg gtggacacca cggtcgagga ctacgatccc gtcaccgccg
      661 ctttccaggt ggcggacgcc atcaatccgc gctcgtcgct gatcgcggtc aaccggatcg
      721 aggggctcac ccgcgcggtg gtggcgccgg aggcgggcga cagcctgatc gccggccagg
      781 ccgccatcat tcacctcgga ggcccggggg acttcctgct gcgctccccc gccgccatgt
      841 tcgccgtcct cggcgaggcg ggggcggagc gggcgggcgg cgcccgcggg gccgccctgc
      901 tgcggctgcg cgaggcgctc caggacgccg aggactacgc cgagaaccgc gccgccttcg
      961 agcgcggcga ccgccgcgag tactcggtct cgcggctcga ccttgaagcc ctggtgccgg
     1021 tggtccgcgg cgagctcccg ctggtggtga cggtcaaccg ggcgagcgac atccaggcgg
     1081 cgctgcggct ggcgaaggag ctcgagctga agctgatcct cgccggcgcc aacgagggct
     1141 ggctggtcgc ccgggagatc gcgcaggcca aggtgccggt gctggtgacg ccgatggaga
     1201 accgtccgga gcgcttcgag atcctggccg cgacgctgga gaacacggcc cggctgcacg
     1261 cggcgggcgt caccgtcgcc ctgatgagcg gcgatgcgca caacgcccgc aacatccggc
     1321 aggcggcggg caacgccgtc gcgtacggca tgccctggga cgcggcgctc gccgccatga
     1381 ccgtggtccc cgcccgcctc tggggcctcg ccgaccgcta cggcaccctg gagccaggca
     1441 aggacgcgga cgtggtgatc tgggacggcg acccgctgga gatcaccacc ttcgccgacc
     1501 gggtcttcat ccgcggagtc gaaatcccga tgaggagccg ccagaccgag ctccgcgacc
     1561 gctacaagga cctctccggc gacctgccgc cggcctacag aaacccgtag gcgcctaccc
     1621 cgccgcgccg gagcccgtca tccggcgcgg cgcggcggga cctcacccca tccgcgccgc
     1681 cagcttgtcc acgatcagcc ttccgatgtt cagcgaggcg gtcgcccccg gggagggggc
     1741 gttcaagacg ttgaccaccc tctcgctctc ggcgatcagg aagtcgtcca ccggcagccc
     1801 gtcgggggtc acggcctggg cgcggacgcc ggcgggggcg gggtggaggt gctcctcgcg
     1861 gatgccgggg accaggcgct gcagggcgcg gacgaaggcc ttcttgctga aggagcgcca
     1921 catctccccc atgcccgtcc gccagtggcg gctcgccagg cgcaggaagc cggcataggt
     1981 caggctctcc agcaggtccg ccggatggat gtcccgcttg gtgtagccct cgcgggcgaa
     2041 ggccagcaca gcgttgggcc cgcactcgac gccaccgtgg atcatccgcg tgaagtgcac
     2101 ccccaggaag gggaagctgg ggtcgggcac cgggtagatc aggttgcggc agaggggcca
     2161 ggcgtcgcgc tccagctcga agtactcgcc gcggaagggg acgatcttcg cgccggggtc
     2221 ctggccggag agcttcgcca cgcggtcgca gtagaggccg gcgcagttca ccacgtagcc
     2281 ggcctcgtag tcgccggcct cggtctccac ggtgacggtg gggtcgatac ggcggatgcc
     2341 ggtcacccgg gcgccggtca ccaccgcacc gccactctcg cggatccgct gcacgagccg
     2401 gcggcagacc tcgggatagt cgacgatgcc ggtctccggc acgtggatgg cccggatgcc
     2461 ggcggcgtgc ggctccagct cgcgcaggcg ctccgggccg atcacctcgc agcgcacgcc
     2521 gttctcctgg ccgcgctcgt ggatccgcgc gagcgccggc agctccggct cgccgagcgc
     2581 cacgatcacc ttgccgcaca gctcgtaggc gatcccctcg cgggcacaga actcctccat
     2641 ggcccgcttc ccctcgcggc aattgaccgc cttgagggag cccggcttgt agtagatccc
     2701 cgagtggagc acccccgagt tgcggccggt ctggtggagc gccacctccg gctccttctc
     2761 cagcaccacc acccgccgcc cagggtaccg ctcggcgaac cggtacgccg tcgccaggcc
     2821 cacgatgccg ccgcccacga tggcgacgtc cgccgtccgc gcgttcatgg attcgtccgt
     2881 cctctcttcc aggtggcgga ttctagcgga cggcgggctg cctgaggcgc ccgagcaccg
     2941 cgttgaagtc agccggcggc ggcgcctcga agcggacggc gcggccgctg cgagggtgga
     3001 ggaagcccag gaccgtggcg tggagcatcg ggcgtggcgc cggcaggcgg gggccgcgcc
     3061 agtcgcggat gtagaccgcc tcccccacca gcgggtgccc cgcctcggag aggtggatgc
     3121 ggatctggtg ctggcggccg gtctccagct cgcaggccac cagggtcgcc tccgcgagcc
     3181 gctccagcgc ctcgacgcgg gtgaccgccg gcttggcgtc cggcggcggc tccccccggc
     3241 cgtgccgccg ggggttccac gagccccgca ggccgtcccc ccggtcggcg agcagcagcg
     3301 tgtcgaaggt ggcgctctcc acccggccgt gggcgatggc gaggtagcgg cgggtcacat
     3361 cgtgacgccc gagctgctct tgtaaatgac gctgagcgcg cacgttgcgg gcgaagacca
     3421 ccagccccga ggtctccttg tcgaggcgct ggaccgcccg caaggtcggc ttggggtctc
     3481 cgaggcgccg gacggccacg cgggtgaagg cgaggagcgt gtcgcggtcg tcgcgctcga
     3541 aggggacggt gaggaggccc gcgggcttgt tcaccaccac cacgtcggcg tccaggtgga
     3601 ccacggcgtc cggcggcagg gggccgcgct gaccccggag gacagcgggg ccggcgagcc
     3661 gcagctcgac gcgctcgccg gcgatggcgc ggcgtgccgg gtcggtctcg acggcgccgt
     3721 cgaccagcac gcggccggcg cggcagaggt cccgcgcctt gctccaggag gtcccctcgc
     3781 cggcgatctc ccgcaccaga gcggcgaggg tgaggccggc tcggtcccct tcgaccgtga
     3841 gagcgtcctt caagcgcacc tatagtaccc tacgcggtcc ggccgcccct gcggcaccga
     3901 tacccctccg ctccatgatc cgctccgaac ccgtccgcca ggagctccgg gccaccctcc
     3961 gtctggcggc cccggtggtc ctcgtccagc tcgggtccat gttgatgggc atggtggaca
     4021 ccctcatggt cggccacctg tcggccgagg ccctggccgc cgccgccctg gcccacatct
     4081 tcgtcatcac ggtgctgatg ctgggcgccg gcatcctgca ggcgctcgac ccgctgatca
     4141 ctcaggcgta cggcgcgggg gatgtccacg gcgtggggga ccacctcctg cgcggcatcg
     4201 tcatggcggc ggcgctgtcc ctccccatct ccctgctcat gctggacacc cgcgaggccc
     4261 tcgccctgct gggccagcag ccggaggtga tcgacggggc ggcgggctac atccgggccg
     4321 tcatctgggg aaacctcccc ttcttcctgt tcacggtcgt ccgcctcacc ctgcagacgc
     4381 agagcatcgt gcgtcccgcg gtgatggcca tggtgatcgg caacgtggtc aacgtcatcg
     4441 tcaactactc cctgatcttc gggaaattcg gcatgccggc cctcggggta gtgggctcgg
     4501 cgtactcgac cgccctgtcg cgctggtgca tgtgcctgta cctggtgatc gcgtcgcgcc
     4561 gcgccctcgc cccctattgg cggggcttca ccggcgccgc ggcgtcgctc cggcactaca
     4621 cccgtctcct ccggctcggc gtccccatcg gtctccacaa ctcgctggag ctgtgcctgt
     4681 tcctcaccgt cgcactgctc atcggccaga tgggggtcgc ccagctcgcg ggtcatcaga
     4741 tcgccctcaa cctgtccgcc atctccttca tggtgccgct ggggatcggg ggcgccgcgg
     4801 ccacccgggt ggggaacgcg atcggccgcg gggacatgcc gggagcccgc cgtgccgccg
     4861 ccgtctccct cggcctcggc gccggcgcca tggccctgtt cgggctcctc ttcgccagcg
     4921 cgccgcggcc cctcgccagc ctctacacgg acgacgccgc ggtgctcgcc atggccgtgg
     4981 cgctcctccc catcgccgcc gcgttccaga tcttcgacgg cgtgcaggtg gtgtcggccg
     5041 gcatcctgcg aggctcggcg gacaccacgt atccggcggc gctggcgctc ttcgggttct
     5101 gggccctggg cctgcccggc ggctggtaca tggccgtccc cctcggcctc gggccgcgcg
     5161 ggctctggtg gggcctgacc ctcggcctcg gcgccgtatc ggcgctgttc gtcctccgca
     5221 tcgtcacccg cttccgctcg cacatctccc gcgagatggg gtagaaaggg gacatgacac
     5281 cgctccgcct gcaagtcctc gccgagcgct tcgcggtctg ccgcctgccg ccggactccc
     5341 cgatcccccc ccgcccgcag gcctgcggcc tgtggtccgc gactctcacg ggcaacgagc
     5401 tctccctggt gctgcccgag tcagcggcgc cgcctggggt ccccaccgag cgggggtggc
     5461 gggcgctgga ggtcgccggc ccgttggatt tcgcgctgac cggcgtcatg gcctccctct
     5521 cctcccccct ggcggcggcc gggatcagcc tgttcgtcgt ctccacctat gacaccgact
     5581 acgtcctggt gcgggatcac gacctcggcc gcgccaagga ggcgctcgaa gccgcggggc
     5641 atcaggtggc cgaggggccg gggggcggat catgaccttc aaggaccact tctccggcca
     5701 cgcggccgac tatgcctcgt tccggcccgg ctatccggcg gtgctgttcg agcgggtcgc
     5761 cgcgctcccc cgccaccggc gcctagcctg ggactgcggc accggcagcg gccaggcggc
     5821 ggtgggcctc gcggagcatt tcgagcgggt catcgccacc gacccg
//
